bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Autoconversion Parameterizations of Cloud Droplet to Raindrop on Numerical Simulations of a Meiyu Front Heavy Rainfall Event
Authors: Kang Zhaoping , Zhou Zhimin , Sun Yuting , Hu Yang , He Dengxin
Published: June 2023

This study analyzes the different impacts of autoconversion of cloud droplets to raindrops (ACR) in a Meiyu front rainfall event by comparing two simulations using different parameterizations (KK00 and LD04) in the Weather Research and Forecasting (WRF) model. The Meiyu frontal clouds are further classified into stratiform and deep-convective cloud categories, and the precipitation and microphysical characteristics of the two simulations are compared with a budget analysis of raindrops. The simulated precipitation, radar composite reflectivity distribution, and rain rate evolution are overall consistent with observations while precipitation is overestimated, especially in the rainfall centers. The intensity and vertical structure of the ACR process between the two simulations are significantly different. The ACR rate in LD04 is larger than that in KK00 and there are two peak heights in LD04 but only one in KK00. Accretion of droplets by raindrops (CLcr), melting of ice-phase particles (ML), evaporation of raindrops (VDrv), and accretion of raindrops by ice-phase particles (CLri) are the dominant pathways to raindrop production. Limited distributional differences can be found in both the deep-convective and stratiform clouds between the two simulations during the growth stage of the Meiyu event. Stronger ACR in LD04 results in less cloud droplet content (Lc), more raindrop content (Lr), and larger raindrop number concentration (Nr) and the effect of ACR on Nr is greater than that on Lr. The ACR process also impacts other microphysical processes indirectly, and the influences vary in the two cloud categories. Less CLcr (especially), ML, and VDrv content, caused by stronger ACR, lead to less raindrop production in the LD04 deep-convective clouds, which is different from stratiform clouds, and finally correct the overestimated rainfall center to better match the observations.
Lightning Risk Warning Method Using Atmospheric Electric Field Based on EEWT-ASG and Morpho
Authors: Li Xiang , Yang Ling , Yin Qiyuan , Yang Zhipeng , Zhou Fangcong
Published: June 2023

The current methods for lightning risk warnings that are based on atmospheric electric field (AEF) data have a tendency to rely on single features, which results in low robustness and efficiency. Additionally, there is a lack of research on canceling warning signals, contributing to the high false alarm rate (FAR) of these methods. To overcome these limitations, this study proposes a lightning risk warning method that incorporates enhanced empirical Wavelet transform-Adaptive Savitzky–Golay filter (EEWT-ASG) and one-dimensional morphology, using time-frequency domain features obtained through the Wavelet transform (WT). The proposed method achieved a probability of detection (POD) of 77.11%, miss alarm rate (MAR) of 22.89%, FAR of 40.19%, and critical success index (CSI) of 0.51, as evaluated on 83 lightning events. This method can issue a warning signal up to 22 min in advance for lightning processes.
Multi-Task Deep Learning Games: Investigating Nash Equilibria and Convergence Properties
Authors: Lee Minhyeok
Published: June 2023

This paper conducts a rigorous game-theoretic analysis on multi-task deep learning, providing mathematical insights into the dynamics and interactions of tasks within these models. Multi-task deep learning has attracted significant attention in recent years due to its ability to leverage shared representations across multiple correlated tasks, leading to improved generalization and reduced training time. However, understanding and examining the interactions between tasks within a multi-task deep learning system poses a considerable challenge. In this paper, we present a game-theoretic investigation of multi-task deep learning, focusing on the existence and convergence of Nash equilibria. Game theory provides a suitable framework for modeling the interactions among various tasks in a multi-task deep learning system, as it captures the strategic behavior of learning agents sharing a common set of parameters. Our primary contributions include: casting the multi-task deep learning problem as a game where each task acts as a player aiming to minimize its task-specific loss function; introducing the notion of a Nash equilibrium for the multi-task deep learning game; demonstrating the existence of at least one Nash equilibrium under specific convexity and Lipschitz continuity assumptions for the loss functions; examining the convergence characteristics of the Nash equilibrium; and providing a comprehensive analysis of the implications and limitations of our theoretical findings. We also discuss potential extensions and directions for future research in the multi-task deep learning landscape.
Periodic and Almost Periodic Solutions of Stochastic Inertial Bidirectional Associative Memory Neural Networks on Time Scales
Authors: Liu Mingshuo , Dong Huanhe , Zhang Yong , Fang Yong
Published: June 2023

The stochastic inertial bidirectional associative memory neural networks (SIBAMNNs) on time scales are considered in this paper, which can unify and generalize both continuous and discrete systems. It is of primary importance to derive the criteria for the existence and uniqueness of both periodic and almost periodic solutions of SIBAMNNs on time scales. Based on that, the criteria for their exponential stability on time scales are studied. Meanwhile, the effectiveness of all proposed criteria is demonstrated by numerical simulation. The above study proposes a new way to unify and generalize both continuous and discrete SIBAMNNs systems, and is applicable to some other practical neural network systems on time scales.
An Efficient Class of Estimators in Stratified Random Sampling with an Application to Real Data
Authors: Bhushan Shashi , Kumar Anoop , Lone Showkat Ahmad , Anwar Sadia , Gunaime Nevine M.
Published: June 2023

This research article addresses an efficient separate and combined class of estimators for the population mean estimation based on stratified random sampling (StRS). The first order approximated expressions of bias and mean square error of the proposed separate and combined class of estimators are obtained. A comparative study is conducted to determine the efficiency conditions in which the suggested class of estimators outperforms the contemporary estimators. These efficiency conditions are examined through an extensive simulation study by employing a hypothetically drawn symmetrical and asymmetrical populations. The simulation results have shown that the suggested class of estimators is more effective than the other available estimators. In addition, an application of the proposed methods is also presented by examining a real data set.
A π–π Stacked High-Performance Organic Anode for Durable Rocking-Chair Zinc-Ion Battery
Authors: Tang Yuyan , Li Shaohui , Lin Meng-Fang , Chen Jingwei , Eh Alice , Xu Qun
Published: June 2023

Sustainable organic materials have gained considerable attention as electrodes for zinc-ion batteries (ZIB) due to their high theoretical capacity, structural versatility, and environmental friendliness. However, issues of inferior capacities and poor rate performance owing to limited inherent electronic conductivity and severe dissolution still persist. Herein, sandwich-structured perylene diimide-ethylene diamine/graphene (PDI-EDA/EG) composites are judiciously designed and synthesized. The two-dimensional graphene host can interact with the PDI-EDA polymer through π–π stacking, endowing accelerated ion/electron transfer, abundant active sites, excellent structural integrity, and mitigated solubility of the hybrid electrodes. When evaluated as an anode in ZIB, the hybrid electrode delivers a high capacity (110.2 mAh g−1 at 0.1 A g−1), superior rate capability (88.9 mAh g−1 at 5 A g−1), and exceptional durability (93.4% capacity retained after 1000 cycles). The structure evolution of the hybrid electrode during the insertion/extraction cycle was investigated by ex-situ Fourier transform infrared spectra (FTIR) and X-ray photoelectron spectroscopy (XPS), revealing the reversible Zn2+ storage at carbonyl sites. In addition, a prototype rocking-chair ZIB cell was constructed with a zinc pre-intercalated MnO2 cathode, displaying an ultrahigh energy density of 54.9 Wh kg−1 at a power density of 42.5 W kg−1 and excellent stability with negligible capacity decay after 1000 cycles.
Dual-Crosslinking of Gelatin-Based Hydrogels: Promising Compositions for a 3D Printed Organotypic Bone Model
Authors: Shehzad Ahmer , Mukasheva Fariza , Moazzam Muhammad , Sultanova Dana , Abdikhan Birzhan , Trifonov Alexander , Akilbekova Dana
Published: June 2023

Gelatin-based hydrogels have emerged as a popular scaffold material for tissue engineering applications. The introduction of variable crosslinking methods has shown promise for fabricating stable cell-laden scaffolds. In this work, we examine promising composite biopolymer-based inks for extrusion-based 3D bioprinting, using a dual crosslinking approach. A combination of carefully selected printable hydrogel ink compositions and the use of photoinduced covalent and ionic crosslinking mechanisms allows for the fabrication of scaffolds of high accuracy and low cytotoxicity, resulting in unimpeded cell proliferation, extracellular matrix deposition, and mineralization. Three selected bioink compositions were characterized and the respective cell-laden scaffolds were bioprinted. Temporal stability, morphology, swelling, and mechanical properties of the scaffolds were thoroughly studied and the biocompatibility of the constructs was assessed using rat mesenchymal stem cells while focusing on osteogenesis. Experimental results showed that the composition of 1% alginate, 4% gelatin, and 5% (w/v) gelatine methacrylate, was found to be optimal among the examined, with shape fidelity of 88%, large cell spreading area and cell viability at around 100% after 14 days. The large pore diameters that exceed 100 µm, and highly interconnected scaffold morphology, make these hydrogels extremely potent in bone tissue engineering and bone organoid fabrication.
Sialylation: A Cloak for Tumors to Trick the Immune System in the Microenvironment
Authors: Zhou Xiaoman , Chi Kaijun , Zhang Chairui , Liu Quan , Yang Ganglong
Published: June 2023

The tumor microenvironment (TME), where the tumor cells incite the surrounding normal cells to create an immune suppressive environment, reduces the effectiveness of immune responses during cancer development. Sialylation, a type of glycosylation that occurs on cell surface proteins, lipids, and glycoRNAs, is known to accumulate in tumors and acts as a “cloak” to help tumor cells evade immunological surveillance. In the last few years, the role of sialylation in tumor proliferation and metastasis has become increasingly evident. With the advent of single-cell and spatial sequencing technologies, more research is being conducted to understand the effects of sialylation on immunity regulation. This review provides updated insights into recent research on the function of sialylation in tumor biology and summarizes the latest developments in sialylation-targeted tumor therapeutics, including antibody-mediated and metabolic-based sialylation inhibition, as well as interference with sialic acid–Siglec interaction.
Bilirubin-Induced Transcriptomic Imprinting in Neonatal Hyperbilirubinemia
Authors: Llido John Paul , Fioriti Emanuela , Pascut Devis , Giuffrè Mauro , Bottin Cristina , Zanconati Fabrizio , Tiribelli Claudio , Gazzin Silvia
Published: June 2023

Recent findings indicated aberrant epigenetic control of the central nervous system (CNS) development in hyperbilirubinemic Gunn rats as an additional cause of cerebellar hypoplasia, the landmark of bilirubin neurotoxicity in rodents. Because the symptoms in severely hyperbilirubinemic human neonates suggest other regions as privileged targets of bilirubin neurotoxicity, we expanded the study of the potential impact of bilirubin on the control of postnatal brain development to regions correlating with human symptoms. Histology, transcriptomic, gene correlation, and behavioral studies were performed. The histology revealed widespread perturbation 9 days after birth, restoring in adulthood. At the genetic level, regional differences were noticed. Bilirubin affected synaptogenesis, repair, differentiation, energy, extracellular matrix development, etc., with transient alterations in the hippocampus (memory, learning, and cognition) and inferior colliculi (auditory functions) but permanent changes in the parietal cortex. Behavioral tests confirmed the presence of a permanent motor disability. The data correlate well both with the clinic description of neonatal bilirubin-induced neurotoxicity, as well as with the neurologic syndromes reported in adults that suffered neonatal hyperbilirubinemia. The results pave the way for better deciphering the neurotoxic features of bilirubin and evaluating deeply the efficacy of new therapeutic approaches against the acute and long-lasting sequels of bilirubin neurotoxicity.
Biodiversity and Ecosystem Functioning in Naturally and Experimentally Assembled Communities
Authors: Puppe Daniel , Dimitrakopoulos Panayiotis G. , Lu Baorong
Published: June 2023

Numerous studies have proved that biodiversity and ecosystem functioning (BEF) are closely linked [...]
The Relationship between Lifespan of Marine Bivalves and Their Fatty Acids of Mitochondria Lipids
Authors: Istomina Aleksandra Anatolyevna , Zhukovskaya Avianna Fayazovna , Mazeika Andrey Nikolaevich , Barsova Ekaterina Andreevna , Chelomin Victor Pavlovich , Mazur Marina Alexandrovna , Elovskaya Olesya Alexandrovna , Mazur Andrey Alexandrovich , Dovzhenko Nadezda Vladimirovna , Fedorets Yuliya Vladimirovna , Karpenko Alexander Alexandrovich
Published: June 2023

Marine bivalves belonging to the Mytilidae and Pectinidae Families were used in this research. The specific objectives of this study were: to determine the Fatty Acids (FAs) of mitochondrial gill membranes in bivalves with different lifespans, belonging to the same family, and to calculate their peroxidation index; to compare the levels of ROS generation, malondialdehyde (MDA), and protein carbonyls in the mitochondria of gills, in vitro, during the initiation of free-radical oxation; to investigate whether the FAs of mitochondria gill membranes affect the degree of their oxidative damage and the maximum lifespan of species (MLS). The qualitative membrane lipid composition was uniform in the studied marine bivalves, regardless of their MLS. In terms of the quantitative content of individual FAs, the mitochondrial lipids differed significantly. It is shown that lipid matrix membranes of the mitochondria of long-lived species are less sensitive to in vitro-initiated peroxidation compared with the medium and short-lived species. The differences in MLS are related to the peculiarities of FAs of mitochondrial membrane lipids.
Myosin Heavy-Chain Messenger Ribonucleic Acid (mRNA) Expression and Fibre Cross-Sectional Area in Masseter, Digastric, Gastrocnemius and Soleus Muscles of Young and Adult Rats
Authors: Lagou Aikaterini , Schaub Leandra , Ait-Lounis Aouatef , Denes Balazs J. , Kiliaridis Stavros , Antonarakis Gregory S.
Published: June 2023

Different demands on the muscles of mastication may influence their functional profile (size and distribution of muscle fibre types), which may change during growth and maturation, potentially influencing craniofacial growth. The aim of this study was to evaluate mRNA expression and cross-sectional area of masticatory muscle fibres compared with limb muscles in young and adult rats. Twenty-four rats were sacrificed at two different ages, namely 12 at 4 weeks (young) and 12 at 26 weeks (adult). The masseter, digastric, gastrocnemius and soleus muscles were dissected. Gene expression of myosin heavy-chain isoforms Myh7 (MyHC-I), Myh2 (MyHC-IIa), Myh4 (MyHC-IIb) and Myh1 (MyHC-IIx) in the muscles was measured using qRT-PCR RNA analysis, and immunofluorescence staining was performed to measure the cross-sectional area of different muscle fibre types. Different muscle types and ages were compared. Significant differences were found in the functional profile between masticatory and limb muscles. For the masticatory muscles, there was an increase in Myh4 expression with age, and this change was more intense for the masseter muscles, which also presented an increase in Myh1 expression, similarly to limb muscles. The fibre cross-sectional area of the masticatory muscles was generally smaller in young rats; however, this difference was less pronounced than in limb muscles.
Assessing B-Z DNA Transitions in Solutions via Infrared Spectroscopy
Authors: Duan Mengmeng , Li Yalin , Zhang Fengqiu , Huang Qing
Published: June 2023

Z-DNA refers to the left-handed double-helix DNA that has attracted much attention because of its association with some specific biological functions. However, because of its low content and unstable conformation, Z-DNA is normally difficult to observe or identify. Up to now, there has been a lack of unified or standard analytical methods among diverse techniques for probing Z-DNA and its transformation conveniently. In this work, NaCl, MgCl2, and ethanol were utilized to induce d(GC)8 from B-DNA to Z-DNA in vitro, and Fourier transform infrared (FTIR) spectroscopy was employed to monitor the transformation of Z-DNA under different induction conditions. The structural changes during the transformation process were carefully examined, and the DNA chirality alterations were validated by the circular dichroism (CD) measurements. The Z-DNA characteristic signals in the 1450 cm−1–900 cm−1 region of the d(GC)8 infrared (IR) spectrum were observed, which include the peaks at 1320 cm−1, 1125 cm−1 and 925 cm−1, respectively. The intensity ratios of A1320/A970, A1125/A970, and A925/A970 increased with Z-DNA content in the transition process. Furthermore, compared with the CD spectra, the IR spectra showed higher sensitivity to Z-DNA, providing more information about the molecular structure change of DNA. Therefore, this study has established a more reliable FTIR analytical approach to assess BZ DNA conformational changes in solutions, which may help the understanding of the Z-DNA transition mechanism and promote the study of Z-DNA functions in biological systems.
Using a Quantitative High-Throughput Screening Platform to Identify Molecular Targets and Compounds as Repurposing Candidates for Endometriosis
Authors: Churchill Molly L. , Holdsworth-Carson Sarah J. , Cowley Karla J. , Luu Jennii , Simpson Kaylene J. , Healey Martin , Rogers Peter A. W. , Donoghue J. F.
Published: June 2023

Endometriosis, defined as the growth of hormonally responsive endometrial-like tissue outside of the uterine cavity, is an estrogen-dependent, chronic, pro-inflammatory disease that affects up to 11.4% of women of reproductive age and gender-diverse people with a uterus. At present, there is no long-term cure, and the identification of new therapies that provide a high level of efficacy and favourable long-term safety profiles with rapid clinical access are a priority. In this study, quantitative high-throughput compound screens of 3517 clinically approved compounds were performed on patient-derived immortalized human endometrial stromal cell lines. Following assay optimization and compound criteria selection, a high-throughput screening protocol was developed to enable the identification of compounds that interfered with estrogen-stimulated cell growth. From these screens, 23 novel compounds were identified, in addition to their molecular targets and in silico cell-signalling pathways, which included the neuroactive ligand–receptor interaction pathway, metabolic pathways, and cancer-associated pathways. This study demonstrates for the first time the feasibility of performing large compound screens for the identification of new translatable therapeutics and the improved characterization of endometriosis molecular pathophysiology. Further investigation of the molecular targets identified herein will help uncover new mechanisms involved in the establishment, symptomology, and progression of endometriosis.
ESAT-6 a Major Virulence Factor of Mycobacterium tuberculosis
Authors: Anes Elsa , Pires David , Mandal Manoj , Azevedo-Pereira José Miguel
Published: June 2023

Mycobacterium tuberculosis (Mtb), the causative agent of human tuberculosis (TB), is one of the most successfully adapted human pathogens. Human-to-human transmission occurs at high rates through aerosols containing bacteria, but the pathogen evolved prior to the establishment of crowded populations. Mtb has developed a particular strategy to ensure persistence in the host until an opportunity for transmission arises. It has refined its lifestyle to obviate the need for virulence factors such as capsules, flagella, pili, or toxins to circumvent mucosal barriers. Instead, the pathogen uses host macrophages, where it establishes intracellular niches for its migration into the lung parenchyma and other tissues and for the induction of long-lived latency in granulomas. Finally, at the end of the infection cycle, Mtb induces necrotic cell death in macrophages to escape to the extracellular milieu and instructs a strong inflammatory response that is required for the progression from latency to disease and transmission. Common to all these events is ESAT-6, one of the major virulence factors secreted by the pathogen. This narrative review highlights the recent advances in understanding the role of ESAT-6 in hijacking macrophage function to establish successful infection and transmission and its use as a target for the development of diagnostic tools and vaccines.
Effect of Methyl Jasmonate on the Growth and Biosynthesis of C13- and C14-Hydroxylated Taxoids in the Cell Culture of Yew (Taxus wallichiana Zucc.) of Different Ages
Authors: Demidova Elena , Globa Elena , Klushin Andrey , Kochkin Dmitry , Nosov Alexander
Published: June 2023

The effects of methyl jasmonate (MeJ) on growth and taxoid formation in the cell culture of Taxus wallichiana were investigated to elucidate the specifics of phytohormone action in dedifferentiated plant cells in vitro. The characteristics of the same suspension cell culture were compared in 2017 (the «young» culture) and in 2022 (the «old» culture)—1.5 or 6 years after culture induction, respectively. MeJ (100 µM) is added to the cell suspension at the end of the exponential growth phase. Cell culture demonstrated good growth (dry weight accumulation 10–18 g/L, specific growth rate µ = 0.15–0.35 day−1) regardless of its «age», cultivation system, and MeJ addition. UPLC-ESI-MS analysis revealed the presence of C14-hydroxylated taxoids (yunnanxane, taxuyunnanine C, sinenxane C, and sinenxane B) in the cell biomass. The content of C14-OH taxoids increased from 0.2–1.6 mg/gDW in «young» culture to 0.6–10.1 mg/gDW in «old» culture. Yunnanxane was the main compound in «young» culture, while sinenxane C predominated in «old» culture. Without elicitation, small amounts of C13-OH taxoids (<0.05 mg/gDW) were found only in «young» cultures. MeJ addition to «young» culture had no effect on the content of C14-OH taxoids but caused a 10-fold increase in C13-OH taxoid production (up to 0.12–0.19 mg/gDW, comparable to the bark of yew trees). By contrast, MeJ added to «old» culture was not beneficial for the production of C13-OH taxoids but notably increased the content of C14-OH taxoids (1.5–2.0 times in flasks and 5–8 times in bioreactors). These findings suggest that hormonal signaling in dedifferentiated yew cells grown in vitro is different from that in plants and can be affected by the culture’s age. This might be a result of the high level of culture heterogeneity and constant auto-selection for intensive proliferation, which leads to the predominant formation of C14-OH taxoids versus C13-OH taxoids and a modified cell response to exogenous MeJ treatment.
Septic Tibial Nonunions on Proximal and Distal Metaphysis—A Systematic Narrative Review
Authors: Sidiropoulos Konstantinos , Panagopoulos Andreas , Tsikopoulos Konstantinos , Saridis Alkis , Assimakopoulos Stelios F. , Kouzelis Antonis , Vrachnis Ioannis N. , Givissis Panagiotis
Published: June 2023

Background: Infected nonunion of the tibia represents a challenging complication for orthopedic surgeons and poses a major financial burden to healthcare systems. The situation is even more compounded when the nonunion involves the metaphyseal region of long bones, a rare yet demanding complication due to the poor healing potential of infected cancellous bone; this is in addition to the increased likelihood of contamination of adjacent joints. The purpose of this study was to determine the extent and level of evidence in relation to (1) available treatment options for the management of septic tibial metaphyseal nonunions; (2) success rates and bone healing following treatment application; and (3) functional results after intervention. Methods: We searched the MEDLINE, Embase, and CENTRAL databases for prospective and retrospective studies through to 25 January 2021. Human-only studies exploring the efficacy of various treatment options and their results in the setting of septic, quiescent, and metaphyseal (distal or proximal) tibia nonunions in the adult population were included. For infection diagnosis, we accepted definitions provided by the authors of source studies. Of note, clinical heterogeneity rendered data pooling inappropriate. Results: In terms of the species implicated in septic tibial nonunions, staphylococcus aureus was found to be the most commonly isolated microorganism. Many authors implemented the Ilizarov external fixation device with a mean duration of treatment greater than one year. Exceptional or good bone and functional results were recorded in over 80% of patients, although the literature is scarce and possible losses of the follow-up were not recorded. Conclusion: A demanding orthopedic condition that is scarcely studied is infected metaphyseal tibial nonunion. External fixation seems promising, but further research is needed. Systematic Review Registration: PROSPERO No. CRD42020205781.
Gender Differences in Response to COVID-19 Infection and Vaccination
Authors: Zaher Kawther , Basingab Fatemah , Alrahimi Jehan , Basahel Kholood , Aldahlawi Alia
Published: June 2023

Since COVID-19 first appeared, a number of follow-up events have taken place. In an effort to find a solution to this catastrophe, a great deal of study and analysis has been conducted. Because of the high morbidity and exceptionally large losses, scientists are being pushed to conduct more research and find vaccination and treatments. The virus has a wide range of effects, one of which is how it affects sexual activity in both men and women. The impact of the cardiovascular system and susceptibility to embolism, lung stress, and infection heightens the probability of hospitalization in the intensive care unit for pregnant women who have contracted COVID-19. There is no evidence of infection being passed from mother to child. In the current review, the role of COVID-19 infection and vaccination on male and female sexual activity, hormones, and the menstrual cycle for females, as well as on male sex hormones and sexual activity during infection and after vaccination, are being investigated. There are no reports of the virus being isolated from the semen of an infected patient or recently recovered patients. A recent investigation on the influence of the virus on gender susceptibility to sexual organs and function has been uncovered throughout this study.
Auxological and Metabolic Parameters of Children Undergoing the Gonadotropin-Releasing Hormone Stimulation Test: Correlations with the Final Diagnosis of Central Precocious Puberty in a Single-Center Study
Authors: Cipolla Clelia , Sodero Giorgio , Pane Lucia Celeste , Mariani Francesco , Di Sarno Lorenzo , Rigante Donato , Candelli Marcello
Published: June 2023

Background—Central precocious puberty (CPP) is characterized by clinical, biochemical, and radiological features similar to those of normal puberty, but CPP occurs before the age of eight in girls and before the age of nine in boys, subsequently leading to a reduction in the final body height in adulthood due to premature fusion of growth plates. The diagnosis of CPP is confirmed with a gonadotropin-releasing hormone (GnRH) stimulation test, which can lead to different interpretations because the diagnostic peak levels of luteinizing hormone (LH) can vary. Patients and methods—This was a single-center, retrospective observational study investigating the possible correlation between gonadotropin peaks on the GnRH test and auxological, metabolic, and radiological parameters of patients evaluated for CPP. We collected and analyzed data from the medical records of children with suspected CPP over a period from January 2019 to July 2022 who underwent a GnRH test at the Fondazione Policlinico Universitario Agostino Gemelli in Rome, Italy. Results—Our correlation analysis revealed no statistically significant differences in any auxological and radiological parameters. Among laboratory parameters, baseline levels of LH, follicle-stimulating hormone, sex hormone-binding globulin, and 17-beta estradiol were higher in children with a definitive diagnosis of CPP than in those with a negative GnRH test. In particular, the levels of LH at baseline and after the GnRH test were statistically significant in the group of CPP patients, consistent with the interpretation of the test. In the multivariate analysis, using a cut-off value of 4.1 IU/L, LH peaks showed both very high sensitivity (94%) and very high specificity (95%); all other variables showed high specificity (90%) but unsatisfactory sensitivity. Conclusion—Basal hormone dosages and, especially, basal levels of LH should be considered before performing a GnRH test as they might anticipate the final diagnosis of CPP.
A Novel Polyurethane-Based Polyion Complex Material with Tunable Selectivity against Interferents for Selective Dopamine Determination
Authors: Zhang Zixin , Guo Hongchen , Hirai Yuugo , Takeda Katsunori , Asai Chiho , Takamura Naohiro , Niwa Osamu
Published: June 2023

Polyion complex (PIC) materials have been widely used in biosensors due to their molecular selectivity. However, achieving both widely controllable molecular selectivity and long-term solution stability with traditional PIC materials has been challenging due to the different molecular structures of polycations (poly-C) and polyanions (poly-A). To address this issue, we propose a novel polyurethane (PU)-based PIC material in which the main chains of both poly-A and poly-C are composed of PU structures. In this study, we electrochemically detect dopamine (DA) as the analyte and L-ascorbic acid (AA) and uric acid (UA) as the interferents to evaluate the selective property of our material. The results show that AA and UA are significantly eliminated, while DA can be detected with a high sensitivity and selectivity. Moreover, we successfully tune the sensitivity and selectivity by changing the poly-A and poly-C ratios and adding nonionic polyurethane. These excellent results were employed in the development of a highly selective DA biosensor with a detection range from 500 nM to 100 μM and a 3.4 μM detection limit. Overall, our novel PIC-modified electrode has the potential to advance biosensing technologies for molecular detection.
Modulating Individual Alpha Frequency through Short-Term Neurofeedback for Cognitive Enhancement in Healthy Young Adults
Authors: Li Ben-Zheng , Nan Wenya , Pun Sio Hang , Vai Mang I. , Rosa Agostinho , Wan Feng
Published: June 2023

Human alpha oscillation (7–13 Hz) has been extensively studied over the years for its connection with cognition. The individual alpha frequency (IAF), defined as the frequency that provides the highest power in the alpha band, shows a positive correlation with cognitive processes. The modulation of alpha activities has been accomplished through various approaches aimed at improving cognitive performance. However, very few studies focused on the direct modulation of IAF by shifting the peak frequency, and the understanding of IAF modulation remains highly limited. In this study, IAFs of healthy young adults were up-regulated through short-term neurofeedback training using haptic feedback. The results suggest that IAFs have good trainability and are up-regulated, also that IAFs are correlated with the enhanced cognitive performance in mental rotation and n-back tests compared to sham-neurofeedback control. This study demonstrates the feasibility of self-regulating IAF for cognition enhancement and provides potential therapeutic benefits for cognitive-impaired patients.
The Role of Stimuli-Driven and Goal-Driven Attention in Shopping Decision-Making Behaviors—An EEG and VR Study
Authors: Saffari Farzad , Zarei Sahar , Kakaria Shobhit , Bigné Enrique , Bruni Luis E. , Ramsøy Thomas Z.
Published: June 2023

The human attention system, similar to other networks in the brain, is of a complex nature. At any moment, our attention can shift between external and internal stimuli. In this study, we aimed to assess three EEG-based measures of attention (Power Spectral Density, Connectivity, and Spectral Entropy) in decision-making situations involving goal-directed and stimulus-driven attention using a Virtual Reality supermarket. We collected the EEG data of 29 participants in 2 shopping phases, planned and unplanned purchases. The three mentioned features were extracted and a statistical analysis was conducted. We evaluated the discriminatory power of these features using an SVM classifier. The results showed a significant (p-value < 0.001) increase in theta power over frontal, central, and temporal lobes for the planned purchase phase. There was also a significant decrease in alpha power over frontal and parietal lobes in the unplanned purchase phase. A significant increase in the frontoparietal connectivity during the planned purchase was observed. Additionally, an increase in spectral entropy was observed in the frontoparietal region for the unplanned purchase phase. The classification results showed that spectral entropy has the highest discriminatory power. This study can provide further insights into the attentional behaviors of consumers and how their type of attentional control can affect their decision-making processes.
A Visual Speech Intelligibility Benefit Based on Speech Rhythm
Authors: Kawase Saya , Davis Chris , Kim Jeesun
Published: June 2023

This study examined whether visual speech provides speech-rhythm information that perceivers can use in speech perception. This was tested by using speech that naturally varied in the familiarity of its rhythm. Thirty Australian English L1 listeners performed a speech perception in noise task with English sentences produced by three speakers: an English L1 speaker (familiar rhythm); an experienced English L2 speaker who had a weak foreign accent (familiar rhythm), and an inexperienced English L2 speaker who had a strong foreign accent (unfamiliar speech rhythm). The spoken sentences were presented in three conditions: Audio-Only (AO), Audio-Visual with mouth covered (AVm), and Audio-Visual (AV). Speech was best recognized in the AV condition regardless of the degree of foreign accent. However, speech recognition in AVm was better than AO for the speech with no foreign accent and with a weak accent, but not for the speech with a strong accent. A follow-up experiment was conducted that only used the speech with a strong foreign accent, under more audible conditions. The results also showed no difference between the AVm and AO conditions, indicating the null effect was not due to a floor effect. We propose that speech rhythm is conveyed by the motion of the jaw opening and closing, and perceivers use this information to better perceive speech in noise.
miRNA Expression Analysis of the Hippocampus in a Vervet Monkey Model of Fetal Alcohol Spectrum Disorder Reveals a Potential Role in Global mRNA Downregulation
Authors: Gillis Rob F. , Palmour Roberta M.
Published: June 2023

MicroRNAs (miRNAs) are short-length non-protein-coding RNA sequences that post-transcriptionally regulate gene expression in a broad range of cellular processes including neuro- development and have previously been implicated in fetal alcohol spectrum disorders (FASD). In this study, we use our vervet monkey model of FASD to follow up on a prior multivariate (developmental age × ethanol exposure) mRNA analysis (GSE173516) to explore the possibility that the global mRNA downregulation we observed in that study could be related to miRNA expression and function. We report here a predominance of upregulated and differentially expressed miRNAs. Further, the 24 most upregulated miRNAs were significantly correlated with their predicted targets (Target Scan 7.2). We then explored the relationship between these 24 miRNAs and the fold changes observed in their paired mRNA targets using two prediction platforms (Target Scan 7.2 and miRwalk 3.0). Compared to a list of non-differentially expressed miRNAs from our dataset, the 24 upregulated and differentially expressed miRNAs had a greater impact on the fold changes of their corresponding mRNA targets across both platforms. Taken together, this evidence raises the possibility that ethanol-induced upregulation of specific miRNAs might contribute functionally to the general downregulation of mRNAs observed by multiple investigators in response to prenatal alcohol exposure.
Association of Motoric Cognitive Risk Syndrome with Sarcopenia and Systemic Inflammation in Pre-Frail Older Adults
Authors: Merchant Reshma Aziz , Chan Yiong Huak , Anbarasan Denishkrshna , Aprahamian Ivan
Published: June 2023

Motoric cognitive risk syndrome (MCR) is defined by the presence of slow gait and subjective cognitive decline. It is well recognized as a prodrome for dementia, but the biological mechanism and trajectory for MCR are still lacking. The objective of this study was to explore the association of MCR with body composition, including sarcopenia and systemic inflammation, in pre-frail older adults in a cross-sectional study of 397 pre-frail community-dwelling older adults. Data on demographics, physical function, frailty, cognition (Montreal Cognitive Assessment (MoCA)), perceived health and depression were collected. Body composition was measured using a bioelectrical impedance analyzer. Systemic inflammatory biomarkers, such as progranulin, growth differentiation factor-15 (GDF-15), interleukin-10 (IL-10), interleukin-6 and tumor necrosis factor-α (TNF-α), were collected. Univariate and multivariate logistic regression were used to analyze the association between MCR, body composition, sarcopenia and systemic inflammatory biomarkers. The prevalence of MCR was 14.9%. They were significantly older and there were more females, depression, functional impairment, lower education, physical activity and MoCA scores. Body fat percentage (BF%), fat mass index, fat to fat free mass ratio (FM/FFM) and sarcopenia prevalence were significantly higher in MCR. Serum GDF-15 and TNF-α levels were highest with progranulin/TNF-α and IL-10/TNF-α ratio lowest in MCR. Compared to healthy patients, MCR was significantly associated with sarcopenia (aOR 2.62; 95% CI 1.46–3.17), BF% (aOR 1.06; 95% CI 1.01–1.12), FMI (aOR 1.16; 95% CI 1.02–1.30) and FM/FFM (aOR 6.38; 95% CI 1.20–33.98). The association of IL-10 to TNF-α ratio (aOR 0.98, 95% CI 0.97–0.99) and IL-10 (aOR 2.22, 95% CI 0.05–0.98) with MCR were independent of sarcopenia and BF%. Longitudinal population studies are needed to understand the role of body fat indices and IL-10 in pre-frail older adults with MCR and trajectory to dementia.
Factors Influencing Participation in Physical Activity, Sports, and Exercise in Children and Adolescents with Spinal Pain or Spinal Conditions: A Systematic Review and Meta-Ethnography
Authors: Tucker Susanna , Heneghan Nicola R. , Gardner Adrian , Rushton Alison , Alamrani Samia , Soundy Andrew
Published: June 2023

Background: Physical activity is an effective treatment for paediatric spinal pain. However, participation rates remain low and review evidence is needed to establish why. This review identifies factors influencing participation in sports, exercise, and physical activity in those aged 18 or under with spinal pain or spinal conditions. Trends or differences between discrete sub-populations are identified. Methods: A meta-ethnographic review was undertaken. Qualitative papers were identified and appraised using the JBI checklist. Thematic trends were mapped onto the biopsychosocial model and subthemes identified. Uniqueness was calculated and the confidence in the evidence was evaluated using the GRADE-CERQual tool. Results: Data were gathered from nine qualitative papers (384 participants). Three themes were identified: (1) biological: physical challenges and bladder and bowel care; (2) psychological: perceptions of differences to peers, struggle, anger, sadness, adjustment, and acceptance; and (3) sociological: influence of friends, social acceptance, negative attitudes from others, and the influence of their disability on family routine. Conclusions: Sociological factors were most influential on exercise participation alongside related psychological and biological factors. Adolescents over 14 years offered greater critical insight compared to the younger children. Results are best applied to neuromuscular conditions with further robust evidence required in paediatric musculoskeletal spinal pain.
Assessing the Validity and Reliability of a French Version of the Child Feeding Questionnaire among Black Mothers Living in Ottawa (Canada)
Authors: Bodnaruc Alexandra M. , Kengneson Cris-Carelle , Blanchet Rosanne , Giroux Isabelle
Published: June 2023

The Child Feeding Questionnaire (CFQ) is a well-established tool used to assess parental beliefs, attitudes, and child feeding practices, with a focus on childhood obesity proneness. To date, there is no French version of the CFQ, nor any Canadian studies assessing its construct validity. The aim of this study was to assess the construct validity and the reliability of a French version of the CFQ among Black mothers (n = 136) of school-aged children living in Ottawa (Canada). The final best fitting model included 7 factors, 20 items, and 1 error covariance. This model was retained as the final model as it (1) excluded two items with very low factor loadings; (2) had the lowest χ2, AIC, BIC, RMSEA, and SRMR values; and (3) had CFI and TLI values ≥ 0.95. Internal consistency ranged from poor to good; the restriction subscale had the lowest internal consistency, followed by the perceived responsibility, pressure to eat, perceive child weight, concern about child weight, and monitoring scales, respectively. Our results showed that a seven-factor model with minor modifications was best fitted to the current data. Future studies are needed to test the validity and reliability of the CFQ in other population groups and among fathers.
Dual-Task Performance, Balance and Aerobic Capacity as Predictors of Falls in Older Adults with Cardiovascular Disease: A Comparative Study
Authors: Silveira Heitor , Lima Juliana , Plácido Jessica , Ferreira José Vinícius , Ferreira Renan , Laks Jerson , Deslandes Andrea
Published: June 2023

Cardiovascular diseases (CVD) are highly prevalent and strongly associated with the risk of falls in the elderly. Falls are associated with impairments in cognition and functional or gait performance; however, little is known about these associations in the elderly population with CVD. In this study, we aimed to clarify the possible associations of physical capacity and functional and cognitive outcomes with the incidence of falls in older adults with CVD. In this comparative study, 72 elderly patients were divided into fallers (n = 24 cases) and non-fallers (n = 48 controls) according to the occurrence of falls within one year. Machine learning techniques were adopted to formulate a classification model and identify the most important variables associated with the risk of falls. Participants with the worst cardiac health classification, older age, the worst cognitive and functional performance, balance and aerobic capacity were prevalent in the case group. The variables of most importance for the machine learning model were VO2max, dual-task in seconds and the Berg Scale. There was a significant association between cognitive-motor performance and the incidence of falls. Dual-task performance, balance, and aerobic capacity levels were associated with an increased risk of falls, in older adults with CVD, during a year of observation.
Research on the Diffusion Model of Cable Corrosion Factors Based on Optimized BP Neural Network Algorithm
Authors: Li Shiya , Yao Guowen , Wang Wei , Yu Xuanrui , He Xuanbo , Ran Chongyang , Long Hong
Published: June 2023

Corrosion factors enter the cable via diffusion and penetration from the defect position of the cable or the connection position between the anchoring system and the cable section, seriously affecting the cable’s durability. Exploring the transmission mechanism of corrosion factors in the cable structure is essential to reveal the durability and the long-term performance of the cable structure and to judge the corrosion damage of steel wires in the cable structure. Based on the machine learning (ML) method and the analytical solution of Fick’s second law, the laws between different temperatures, humidity, cable inclinations, cable defect areas, etc., and the diffusion coefficient of corrosion factors and the concentration of surface corrosion factors are obtained, also a spatial diffusion model of corrosion factors is established. According to the research, the optimum simulation result is achieved by employing the optimized back propagation (BP) neural network algorithm, which has a faster convergence speed and better robustness. Although ambient temperature, humidity, and corrosion time all impact the diffusion rate of corrosion factors, the tilt angle of the cable and the size of cable defects are the main factors influencing the diffusion coefficient of corrosion factors and the concentration of surface corrosion factors. The error between the concentration of corrosion factors calculated by the model in this article and the measured values at each spatial point of the cable is controlled within 15%, allowing for the spatial diffusion of corrosion factors to be effectively predicted and evaluated in practical engineering.
Material Performance of Anti-Differential Disturbance Concrete Based on the Vehicle–Bridge Coupling Vibration Theory
Authors: Zeng Guosheng , Shi Xuefei , Song Jun , Zhu Chaoyu
Published: June 2023

For the purpose of splicing widening bridges without interrupting traffic in expressway reconstruction and extension projects, different types of anti-differential disturbance concrete (ADC) were developed. This paper experimentally investigated the mechanical performance of ADC under the influence of traffic–vehicle coupling vibration. The experimental device, with one end fixed and the other end vertically vibrated, was designed to simulate vehicle–bridge coupling vibration on an existing old bridge. Different disturbance degrees were simulated by adopting specimens made with standard molds and integral plate molds. The compressive strength and other performances of normal-type ADC (NADC) and high-performance ADC (HPADC) were tested. Owing to the differential disturbance, the compressive strength of the NADC increased by 10~20%, and that of the HPADC was almost unaffected. The flexural tensile strengths of the NADC and the HPADC were decreased to some extent. NADC and HPADC can both meet the requirement of widening bridge splicing, and the anti-differential disturbance performance of HPADC is superior to that of NADC.