bluuebunny/crossref_metadata_2025 huggingface dataset:
MODERN ASPECTS OF FERTILIZER USING IN CROP PRODUCTION AND RESEARCH OF THEIR IRRATIONAL USE CONSEQUENCES
Authors: BEREZUK Sergey , ZUBAR Ivan
Published: February 2020

The article discusses key aspects of current trends in fertilizer application. A key feature is the inconsistency of the existing fertilizer system with scientifically sound standards. The consequences of the irrationality of nutrient input in the field of crop production are analyzed. It is determined that these trends have caused a number of problems, among which there is a chronic shortage of basic nutrients, soil erosion, growth of areas of land with high acidity, mineralization of humus reserves. Organic and mineral fertilizers and livestock rates have been systematized, which has made it possible to identify trends in farmland fertilization and to identify causal relationships between these indicators. It was found that the negative rate of increase in the livestock population directly affects the volume of fertilizer application and humus content in the upper fertile soil layer.

It is estimated that the dominant situation in the field of production, supply and use of mineral fertilizers in Ukraine does not meet either the needs of the market or the conditions of agriculture in the country, which is due, first of all, to the shortage of potassium and phosphorus components, high cost and a significant share of imports. The consequences of this situation are the inferiority of providing the necessary nutrients for plants, the criticality of this dilemma is proved by the author's alculations of ecological damage, which was carried out on the example of prevailing agricultural plants in the croprotation: corn and winter wheat. The directions of solving the identified range of problems are consolidated in the form of the concept of economic and ecological balance of modern land use.
STATE REGULATION OF RURAL GREEN TOURISM
Authors: RUDA Oksana
Published: February 2020

The article is aimed at highlighting the theoretical foundations of state regulation of rural green tourism in Ukraine. Tourism is increasingly playing the role of an indicator of political relations between the states, a stabilizer of partner relations in the international political arena. Therefore, considering the great importance of tourism, the state has identified it as one of the priority directions of development of the national economy and culture, the sphere of realization of rights and needs of man and society, one of the defining components of the socio-economic policy of the state and regions. Today in Ukraine there are a number of problems that do not contribute to the development of the tourist industry, leading to a significant destruction of economic and social relations in the tourism sector. This article reveals the concept of "rural green tourism", as well as the significance of its development for the country's economy.

The characteristic of the concept of "state regulation of rural green tourism" is given, and the system of methods of regulation is described. The necessity of marketing research as one of ways to improve the system of management of the rural tourism sector is presented. Formulated conclusions that include ways to develop and improve the state regulation of rural green tourism in Ukraine.
Periodization of the history of Ukrainian culture of the nineteenth century: basic models
Authors: Malanchuk-Rybak Oksana
Published: February 2020

The article explores the
historiographical developments concerning the
periodization of the history of Ukrainian culture of the
19th century. The first model is the periodization of
cultural and national revival in 19th century Ukraine.
Main periods: academic (the time of collecting
cultural and historical heritage); organizational (the
time of creation of national cultural and educational
organizations); political (the time of creation of parties
and other organizations that enable the nation to
participate in political processes). The basic idea
of this periodization is to show the processes of
building of the nation and building of the country
in the nineteenth century through the emergence
and development of new phenomena in culture.
The second model is the Pan-European cultural and
historical periodization, which identifies such major
periods of the nineteenth-century cultural history like
Romanticism and Positivism.
miRNA‐mRNA Profiling Reveals Prognostic Impact of SMC1A Expression in Acute Myeloid Leukemia
Authors: Gadewal Nikhil , Kumar Rohit , Aher Swapnil , Gardane Anagha , Gaur Tarang , Varma Ashok K. , Khattry Navin , Hasan Syed K.
Published: February 2020

Acute myeloid leukemia (AML) with NPM1 mutation is a disease driving genetic alteration with good prognosis. Although it has been suggested that NPM1 mutation induces chemosensitivity in leukemic cells, the underlying cause for the better survival of NPM1 mutated
patients is still not clear. Mutant NPM1 AML has a unique microRNA and their target gene (mRNA) signature compared to wild-type NPM1. Dynamic regulation of miRNA‐mRNA has been reported to influence the prognostic outcome. In the present study, in silico expression data
of miRNA and mRNA in AML patients was retrieved from genome data commons, and differentially expressed miRNA and mRNA among NPM1 mutated (n = 21) and NPM1 wild-type (n = 162) cases were identified to establish a dynamic association at
the molecular level. In vitro experiments using high-throughput RNA sequencing were performed on human AML cells carrying NPM1 mutated and wild-type allele. The comparison of in vitro transcriptomics data with in silico miRNA‐mRNA expression network data revealed downregulation
of SMC1A. On establishing miRNA‐mRNA interactive pairs, it has been observed that hsa-mir-215-5p (logFC: 0.957; p = 0.0189) is involved in the downregulation of SMC1A (logFC: ‐0.481; p = 0.0464) in NPM1 mutated AML.
We demonstrated that transient expression of NPM1 mutation upregulates miR-215-5p, which results in downregulation of SMC1A. We have also shown using a rescue experiment that neutralizing miR-215-5p reverses the effect of NPM1 mutation on SMC1A. Using the leukemic
blasts from AML patients, we observed higher expression of miR-215-5p and lower expression of SMC1A in NPM1 mutated patients compared to wild-type cases. The overall survival of AML patients was significantly inferior in SMC1A high expressers compared to low expressers
(20.3% vs. 58.5%, p = 0.018). The data suggest that dynamic miR-215-SMC1A regulation is potentially modulated by NPM1 mutation, which might serve as an explanation for the better outcome in NPM1 mutated AML.
Simultaneous Removal of Lead and Nickel Ions from Aqueous Synthetic Solutions by Chitosan Coated Cobalt Ferrite
Authors: Patescu Rodica Elena , Simonescu Claudia Maria , Onose Cristian , Busuioc Teodor Laurentiu , Pasarica Daiana Elena , Deleanu Carmen
Published: February 2020

This research study deals with lead and nickel simultaneous removal from aqueous solutions by the use of chitosan coated cobalt ferrite as adsorbent. Batch removal tests were performed in order to establish the main parameters that influence the sorption capacity, removal efficiency and the selectivity of this adsorbent. The values of sorption capacity for lead and nickel experimentally determined are: 56.23 mg/g and respectively 45.11 mg/g. Langmuir and Freundlich adsorption isotherms were used to interpret the sorption experimental data. The kinetic data were explored by pseudo-first order, pseudo-second order and intraparticle diffusion kinetic models. The experimental data were well fitted with the pseudo-second order model for both heavy metals. The main conclusion that can be drawn from this research is that this material can be successfully used for the removal of lead and nickel from binary aqueous solutions and wastewater.
Monitoring of the Inorganic Pollutants in Built Indoor Environment
Authors: Vasile Vasilica , Dima Alina , Ion Mihaela
Published: February 2020

The paper presents the results of the monitoring of the inorganic gaseous pollutants such as nitric oxide (NO), sulfur dioxide (SO2), ozone (O3), carbon oxides (CO, CO2), from indoor air of four office spaces, located in urban area of Bucharest � Romania, by using the equipment that detected and recorded, in real time, the concentrations of the compounds. The study aimed to obtain useful information on air quality from office spaces, and for the awareness of acute necessity for action to improve the quality of the indoor environment in which we live and work.
Load-Bearing Capacity of Direct Inlay-Retained Fibre-reinforced Composite Fixed Partial Dentures with Different Cross-Sectional Pontic Design
Authors: Tanculescu Oana , Doloca Adrian , Vieriu Raluca Maria , Mocanu Florentina , Ifteni Gabriela , Vitalariu Anca , Solomon Sorina , Ioanid Nicoleta , Iovan Gianina
Published: February 2020

The load-bearing capacity and fracture pattern of direct inlay-retained FRC FDPs with two different cross-sectional designs of the ponticwere tested. The aim of the study was to evaluate a new fibre disposition. Two types of composites, Filtek Bulk Fill Posterior Restorative and Filtek Z250 (3M/ESPE, St. Paul, MN, USA), and one braided polyethylene fibre, Construct (Kerr, USA) were used. The results of the study suggested that the new tested disposition of the fibres prevented in some extend the delamination of the composite on buccal and facial sides of the pontic and increased the load-bearing capacity of the bridges.
The Impacts of Industrial Processing of Oil on Soil Quality
Authors: Apostolescu Zoia , Chivu Oana Roxana , Semenescu Augustin , Babis Claudiu , Amza Catalin , Iacobescu Gabriel , Gligor Maria Alina , Nitoi Dan , Adir George Mihail
Published: February 2020

Soil, the one of the main components of the environment, is constantly subjected to the action of pollutants existing both in air and water from rainfall and also in groundwater. Soil pollution recorded in the current stage varying degrees of contamination in relation to the demands manifested, chemical contamination occupying a preferential place being pronounced across the globe, regardless of zone. In view of the above purposes, the paper aims to highlight the impact of industrial processing of oil activity on soil quality, near a chemical plant in Constanta County.
Enamel Apperance after Orthodontic Attachment Removal. In vitro SEM analysis
Authors: Muntean Alexandrina , Mesaros Anca Stefania , Porumb Anca , Cuc Stanca , Moldovan Marioara , Balan Adriana
Published: February 2020

The aim of this in vitro study was to assess the enamel surface appearance and Adhesive Remnant Index (ARI) after braces removal. On teeth vestibular surfaces 90 braces were bonded as follows: metallic, ceramics respectively sapphire using as bonding material Opal Seal� (Ultradent, SUA). Teeth were divided in 3 groups (N=30) and stored for 3 months in artificial saliva, tea and Coca-Cola�. For teeth stored in tea and Coca Cola�, ARI express lower values, indicating a risk for enamel injury as fracture occurs at adhesive-enamel interface. From practical point of view, this in vitro study enhance that braces detachment, finishing and polishing are risk manoeuvres for enamel surface. Materials used for collage must present specific properties in order to reduce enamel fracture.
Research Regarding Chemical Composition and the Digestibility of the Mulberry Leaves from Eforie Variety
Authors: Dolis Marius Gheorghe , Simeanu Cristina , Usturoi Alexandru , Simeanu Daniel
Published: February 2020

During the growth of silkworm larvae study, also was done a research which aimed to determine the chemical composition and the digestibility of mulberry leaves from a Romanian variety, Eforie. The results showed that advancing in the vegetation stage at the same time with different periods of the silkworm larvae�s growth, the mulberry leaves experience an aging process being noticed through its quality decreasing from chemical composition point of view. So, for example, the CP which was in average 20.98�0.670% (from DM), decreased during the study with 3.11%; while the CF had an average of 17.91�0.434%, (from DM) increased with 2.29%. Accordingly, with this, the majority of nutrients from mulberry leaves, excepting cellulose, had an increasing of digestibility with 33.94%. It was noticed a continuous digestibility decreasing during the growth period for the CP with 22.79% and with 33.53% for NFE. The nutrients digestibility from the leaves registered a value of 59.20%.
Synthesis, Characterization and Biological Activity of the Manganese Complexes with alfa-ketoglutaric Acid and 1-0(o-tolyl) Biguanide
Authors: Mihalache Madalina , Oprea Ovidiu , Guran Cornelia , Ardelean Ioana Lavinia
Published: February 2020

This paper presents the synthesis and characterization of three complexes of Mn (II,III), with the a-ketoglutaric acid (H2A) and 1- (o-tolyl) biguanide (TB) as ligands. The complexes corresponding formulas are: [Mn(TB)2(H2A)]Cl2 . 0.5C2H5OH (C1), [Mn2(TB)(A)(H2A)2(H2O)2(NO3)2](NO3)2. C2H5OH (C2) and [Mn2(TB)(A)(H2A)2(H2O)2(CH3COO)2](CH3COO)2 . 3H2O (C3), where A is H2A without 2 protons. C1-C3 compounds were characterized by standard physico-chemical methods: chemical elemental analysis, IR spectroscopy, UV-Vis-NIR spectroscopy, molar conductivity and thermal analysis. For the obtained C1-C3 complexes, but also for the ligands (H2A and TB) was tested the antibacterial activity against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 and antitumor activity on HeLa tumour cells. It has been observed a moderate cytotoxic effect of the complexes C1-C3 on growth and metabolic activity of HeLa cells, while the ligands show a very weak effect on them. The obtained complexes inhibits the adhesion on the substrate of bacterial strains Staphylococcus aureus and Pseudomona aeruginosa, which recommends them for possible therapeutic applications.
Does Active Smoking Influence the Second Trimester Biochemical Markers Concentrations?
Authors: Navolan Dan , Birsasteanu Florin , Carabineanu Adrian , Cretu Octavian , Badiu Diana Liana , Ionescu Cringu Antoniu , Mehedintu Claudia , Vladareanu Simona , Craina Marius , Boia Marioara , Ciohat Ioana , Craciunescu Mihaela , Simu Sebastian , Nemescu Dragos
Published: February 2020

Cigarette smoke contains over 7000 different substances some of them exerting harmful effects on embryo and pregnant woman. Nowadays 15 % of adult people and around 10-15% of pregnant women smoke. Previous studies showed that cigarette smoke compounds could exert pharmacodinamic effects and influence some of the second trimester biochemical markers concentration. Therefore there is a need to adjust the reference values of second trimester markers depending of the smoker status. The aim of our study was to analyse which of the markers are influenced by smoking and whether the software used to calculate the risk for aneuploidies is able to counterbalance this influence. Alpha-fetoprotein (AFP), chorionic gonadotropin hormone (hCG) and free estriol (uE3) values were measured in second trimester sera of 1242 pregnant women: 1089 non-smokers and 153 smokers. Only hCG second trimester values were influenced by smoking whereas AFP and uE3 values were not. The correction of medians according to the smoking status was able to counterbalance this effect.
Effects of Process Factors on Carbon Dioxide Reforming of Methane over Ni/SBA-15 Catalyst
Authors: Orbeci Cristina , Parvulescu Oana Cristina , Acceleanu Elena , Dobre Tanase
Published: February 2020

The process of CO2 reformation of CH4 was conducted over a 5% Ni/SBA-15 catalyst under various experimental conditions. Operating temperature (600-750 �C), gas hourly space velocity (4000-12000 hr-1), and CO2/CH4 feed molar ratio (0.67-1.50) were selected as independent parameters (factors). Process performances were evaluated as conversions of CH4 (21.1-79.6%) and CO2 (42.4-98.7%) as well as H2/CO product molar ratio (0.573-0.992). All process performances were enhanced at higher levels of temperature and low values of gas velocity. An increase in feed molar ratio has determined a significant increase in CH4 conversion and a slighter decrease in CO2 conversion and H2/CO molar ratio. A statistical model based on a 23 factorial plan was used to predict the process performances depending on its factors.
Structural Changes in the Superficial Layers of a Non-conventional Treated Steel Subjected to a Wear Process
Authors: Papadatu Carmen Penelopi , Sandu Andrei Victor , Bordei Marian , Sandu Ioan Gabriel
Published: February 2020

It was considered a non - conventional treatment in magnetic field applied before the thermo-chemical treatment (nitro-carburizing in plasma), for one alloyed steel which is used in Machinery building industry. These researches have been made in order to improve the mechanical properties of Chromium-Molybdenum alloyed steel non-conventionally treated in a magnetic field. Through the thermo-magnetic treatment, applied before the plasma nitro-carburizing, the mechanical properties of this material have been improved, especially in this case of the steel with a considered content of Chromium (1.38%). These researches, through X-Ray diffractometry of the behavior of the superficial layers during the dry wear process, completed this work. The diffractometric characteristics of the superficial layers which have been obtained after a complex system of thermo-magnetic treatments followed by a thermo-chemical treatment, the superficial layers content and the behavior of the steel at the wear through dry friction tests were used as criterion. The paper is a review of the researches from the last few years.
Use of Gentian Violet in the Treatment of Severe Radiodermatitis Secondary to Adjuvant Radiotherapy in Breast Cancer
Authors: Munteanu Anca , Condorovici Daniela , Patrascu Alexandru , Tanase Diana
Published: February 2020

Adjuvant radiotherapy is indicated for patients with conservatively or radically operated breast cancer, demonstrating the benefit of this type of treatment for local recurrence rate and survival. Radiodermatitis are radiation-induced skin reactions, a significant side effect of cumulative ionizing radiation in significant doses at the skin level during the treatment of various tumor localizations. This study presents aspects regarding prevention and treatment of radiodermatitis in patients with adjuvant irradiated breast cancer treated in the Regional Institute of Oncology Iasi. In the study group, use of gentian violet has helped to alleviate these side effects of irradiation, both in intensity and duration, with an important impact on the quality of life of the patients.
Lipd Metabolism Abnormalities in Children and Adolescents with HIV/AIDS Treated with Protease Inhibitors
Authors: Jugulete Gheorghita , Iacob Simona , Merisescu Madalina , Luminos Monica
Published: February 2020

In this paper we propose to evaluate the lipid metabolism abnormalities in children and adolescents with HIV/AIDS undergoing treatment with protease inhibitors (PIs). There are over 1000 children and adolescents with HIV infection of various stages registered in the Pediatric Department of the National Institute of Infectious Diseases Prof. Dr. Matei Bals. We have carried out a retrospective study on 200 HIV seropositive children without previous lipid metabolism abnormalities in which antiretroviral treatment was initiated. In these patients we have monitored modifications in lipid metabolism secondary to antiretroviral treatment (triglycerides, cholesterol, and lipids). The majorities of abnormalities of the lipid metabolism were not severe and did not require modification or cessation of treatment. 24 months of treatment later, approximately 23.5% of patients treated with 2 NRTI+PI/r had modifications of the lipid metabolism: 34.6% had hypercholesterolemia, 38.4% had hypertriglyceridemia, and 36.9% had an increase of total serum lipids. Lipid metabolism abnormalities have been more significant in patients treated previously with antiretrovirals, especially those who have received PIs, in comparison to na�ve patients.
Numerical Modelling of Pollutant Dispersion in the Lower Danube River
Authors: Radu Violeta Monica , Diacu Elena , Moncea Mihaela Andreea , Dumitru Florina Diana , Panait Ana Maria , Ionescu Petra
Published: February 2020

This paper presents the numerical modeling results of the pollutant dispersion in the Danube riverbed, using experimental data recorded monthly on the Borcea - Bala - Old Danube sector, for a 2 years period. The water samples were collected from the left bank, center and right bank of the river, and the follwing indicators were analyzed: lead, nickel, chromium, cadmium and copper. Heavy metals were determined by atomic absorption spectrophotometry with graphite furnace (GFAAS). Numerical modeling was performed using Delft3D software, taking into account a reference situation (a scenario regarding the pollutant dispersion for known concentrations) and a scenario that simulates an accidental pollution (where the concentrations of pollutants were increased by 50 % compared to the values corresponding to Class V of water quality according to M.O. 161-2006). In order to properly assess the pollutant dispersion, the construction of the numerical model was developed taking into account a number of complex hydraulic works (bottom sill, dredging operations on the Danube in the area of confluence and downstream, on the whole width of the fairway). The results of numerical modeling generated the pollutant dispersion maps and the dispersion coefficients (indicating the transport of substances). Higher levels of the monitored indicators were observed on Borcea, caused by water coming from different sources of pollution (industry, agriculture, etc.) and discharged at a low flow, limiting the dilution.
New Method for Simultaneous Determination of Ascorbic and Acetylsalicylic Acids in Effervescent Tablets
Authors: Bungau Simona , Tit Delia Mirela , Iovan Ciprian , Copolovici Lucian , Boscencu Rica , Cioca Gabriela , Copolovici Dana
Published: February 2020

The determination of the real concentration in different pharmaceutical drugs represents a very important goal of the pharmaceutical industry. The main aim of the study was to set up a simple, cheap and rapid method in order to determine both ascorbic and acetylsalicylic acid content of effervescent tablets that could be found on the pharmaceutical market, without preliminary separation or extraction. For this purpose, a very simple and reliable clock method based on two Landolt-type systems of reactions, based on potentiometric techniques, has been developed. The method has been tested on different effervescent tablets which contain ascorbic acid and acetylsalicylic acid in different mixture ratio.
Red Clover Characterization: Trifolium patulum Tausch
Authors: Casacchia Teresa , D`ippolito Claudia , Toma Claudia Crina , Uzunov Dimitar , Marrelli Mariangela , Conforti Filomena , Statti Giancarlo
Published: February 2020

Although clovers (Trifolium ssp, Fabaceae) are considered one of the leading crops for cattle grazing, they could also be used as a potential source of bioactive compounds. in fact, they have a long history of use in popular medicine. The Trifolium pratense (Red Clover) is certainly the most common in this family, but endemic or sub endemic italian species are interesting for the investigation of their bioactive compounds content and their potential application. The aim of the study was to investigate the tirfolium pratensis versus Trifolium patulum Tausch, an endemic species of southern appennine. The analysis focused on GC / MS determinations of volatile content, and HPLC determinations of polar compounds. In addition, antioxidant activity tests were performed.
Research on the Effect of Applying the Sacrifice Layer Technique on the Chemical Composition of the Welded Deposits
Authors: Cicic Dumitru Titi , Rontescu Corneliu , Bogatu Ana Maria , Chivu Oana Roxana , Ghionea Ionut Gabriel
Published: February 2020

All vessel-type products operating under extreme conditions of temperature, pressure and corrosive environments, such as those in the power, chemical and petrochemical industries, are subjected to intense chemical / structural degradation processes that may lead to cracks. These may propagate in time, causing the product to fail, generating significant economic and material consequences, leading in some cases even to the loss of human lives. The paper presents some effects of the sacrifice layer repairing technique used to restore the geometrical shape by welding of the area from where the flaw material was removed, on the chemical composition.
The Importance of Immunohistochemistry Techniques in Assessing the Prognosis of Multimodally Treated Breast Cancer
Authors: Munteanu Anca , Patrascu Alexandru , Ferariu Dan
Published: February 2020

The identification of prognostic factors in the invasive breast cancer is particularly important for the therapeutic approach appropriate to the aggression of neoplasia. Determining the impact of multiple prognostic factors and correlating clinical and immunohistochemical factors allow the treatment to be adjusted so as to avoid the over-treatment of less aggressive neoplasms and to select the optimal multimodal neoadjuvant and adjuvant treatment in cancers with increased aggressiveness. Tissue samples obtained by breast tumor biopsy were studied by classical histopathology methods and by immunohistochemistry techniques, followed by the determination of the histological type, degree of tumor differentiation, status of estrogenic and progesterone receptors, Her2 status. Subsequently, the analysis of the particularities of the prognostic factors associated with the cases with aggressive, metastatic evolution was performed.
Determination of pH and Evaluation of the Efficiency of Different Definitive Root Canal Filling Materials in Conservative Treatment of Chronic Apical Periodontitis
Authors: Ivacson Andrea Csinszka Kovacs , Mola Farah Curt , Biris Carmen , Bica Cristina Ioana , Pacurar Mariana , Monea Adriana
Published: February 2020

The phisico-chemical properties of the root canal filling materials play an important role in the outome of the endodontic treatment. Zinc oxide-eugenol, mineral-trioxid-aggregate (MTA), calcim-hydroxide or glass ionomer cement are the most frequently used chemical components of the materials, which assure them antimicrobial, radiopacity and adhesive properties. The high pH value ensures the sealer antibacterial effects which lead to the lowering of the periodontal inflammation and stimulate the healing process. The aim of this study was to determine in vitro the pH value of five of the most used root canal filling materials and to evaluate their efficiency in vivo in conservative treatment of chronic apical periodontitis.According to our results SealApex (Kerr) presents the highest average pH value and the shortest healing period of chronic apical periodontitis.
The Impact of Laparoscopic Sleeve Gastrectomy on Serum Zinc or Copper and Body Composition
Authors: Timofte Daniel , Ochiuz Lacramioara , Ursaru Manuela , Ciuntu Bogdan , Ionescu Lidia , Calu Valentin , Mocanu Veronica , Puia Ion Cosmin
Published: February 2020

The aim of this study was to investigate the impact of bariatric surgery on body composition, serum zinc and serum copper levels in obese patients over a period of 12 months following laparoscopic sleeve gastrectomy. This could be important considering that this procedure has been proven to be an effective tool in achieving absolute weight loss and reducing fat tissue in morbidly obese population. Thus, our present results are confirming some important modifications behind the biochemistry of the bariatric surgery and the aforementioned impact of laparoscopic sleeve gastrectomy on serum zinc or copper and body composition.
Forensic Aspects in Polonium-210 Poisoning
Authors: Diac Madalina , Knieling Anton , Damian Simona Irina , Bulgaru Iliescu Diana , Sandu Ion , Furnica Cristina , Iov Catalin Jan , David Sofia
Published: February 2020

The human body is exposed to radiation resulting from the presence and degradation in the environment of natural radionuclides like uranium and thorium. In laboratories, several radioactive isotopes are being used: uranium-235, iodine-131, cobalt-60, and carbon-14. These radioactive isotopes are useful in scientific (dating human skeletal remains) and medical purposes (radiotherapy/radiodiagnosis), but they are also used in criminal context. Polonium 210 (Po-210) is a natural radioisotope occurring in the natural environment as consequence of uraniu-238 decay chain. The current paper presents a less known subject of lethal poisoning with Po-210 radioisotope, in order to underline the methods used for Po-210 identification in forensic samples. The authors performed a literature review of the scientific researchers published in the last years concerning polonium sources and its use as a poison. Polonium�s effectiveness as a poison relies on its chemical characteristics only to the extent that they determine the isotope�s distribution and retention in organs and tissues; the alpha particles are responsible for the lethal effect. Po-210 poisoning is particular, as it doesn�t leave traces, it can be easily transported, and is not detected by airport scanners. Identifying this compound during a medico-legal autopsy is very difficult, as the post-mortem examination will not yield characteristic aspects, the forensic pathologist being forced to consider this as a potential cause of a rapid death, with significant visceral damage.
The implications of Milrinone Lactate in the Postoperative Clinical Management of Surgical Interventions for Congenital Heart Malformations in Children
Authors: (Ciorba) Puiac Mariana Anisoara , Suciu Horatiu , Ciorba Marius Ilie , Opris Mihaela Maria , Hussein Hussam Al , Hussein Hamida Al , Brinzaniuc Klara
Published: February 2020

The purpose of the study was to prove to efficacy of Milrinone in the management of open heart surgery in children with congenital heart malformations, the link between Milrinone efficacy and the prevention of low cardiac output syndrome and Milrinone side effects. We conducted a retrospective study on a group of 24 patients, admitted to the Tg Mures Emergency Institute for Cardiovascular Diseases and Transplant, between August 2016 and February 2017. Milrinone was administered to children that underwent open heart surgery for different congenital heart malformations, using doses between 0.25 and 0.75 mcg per kg bodyweight, in continuous intravenous drip, before de-clamping of the Aorta, the procedures being conducted in extracorporeal circulation. We recorded demographic data, biological parameters of renal function, myocardial function and hemodynamic parameters, before and after surgery. The administration of Milrinone determined a reduction of incidence of low cardiac output syndrome, registering only 4 deaths, the survival rate being 83.33%, 13 cases presenting complications. Postoperatively we registered a significant improvement of the mean heart rate. Milrinone proved efficient in the re-establishment of hemodynamic parameters in patients with this type of clinical manifestations. Using Milrinone in children that undergo open heart surgery determines a decrease in incidence of low cardiac output syndrome, its� presence in the pharma market being necessary.
Separation and Detection of Caffeine, Theophylline and Theobromine from Coffee Varieties, Carbonated Soft Drinks and Alcoholic Beverages
Authors: Badea Delia Nica , Levai Codrina
Published: February 2020

The paper evaluates the presence of methyl xanthine compounds: caffeine, theophylline, theobromine used as ingredients in carbonated soft drinks or as color and flavor ingredients in alcoholic beverages. The active components extracted from the selected products (coffee, tea, drinks) was separated and identified chromatographically using plates with silica nano -Sil NH2 / UV-254, mobile phase ethanol - water (50: 1, 50: 3, 50: 5; 50: 7; v / v) and 60 F254 plates, mobile phase acetone-toluene-chloroform (40:30:30 v / v). Separated caffeine and identified by TLC was analyzed using a HelWet Packard 5890 Gas Chromatograph equipped with MS 5972 mass detector and spectral library to confirm identification. This simple and rapid TLC, GC / MS instrumental method is useful in controlling traces of methyl xanthine compounds in food as a food safety measure.is useful in controlling traces compound of food products containing methylxanthines as a food safety measure.
NPK Analysis of the Thermal Degradation of Decabromodiphenylether
Authors: Dumitras Mihai , Maftei Dan , Airinei Anton , Tudorachi Nita , Dirtu Alin Constantin
Published: February 2020

This study aims to investigate the thermal degradation behavior of decabromodiphenyl ether (BDE 209) by simultaneous TG/DTA under various conditions. Due to low volatility, high elution temperatures are needed when applying chromatographic techniques for this class of flame retardants (FR), resulting in thermal degradation affecting analysis results, but no systematic study has been reported. Kinetic analysis was performed by the non-parametric (NPK) method, and in correlation with evolved gas analysis (EGA) information and molecular modeling results, provided valuable insight into the kinetics and mechanism of BDE 209 thermal degradation. The conclusions were aimed at designing proper specific GC analytical methods for the selected FRs and serving for a better estimation of the human exposure to such compounds.
New heterocyclic compounds from 1,2,4-trizoles and 1,3,4-oxadiazoles class containing 5H-dibenzo[a,d][7]annulene moiety
Authors: Socea Laura Ileana , Barbuceanu Stefania Felicia , Draghici Constantin , Nitulescu George Mihai , Saramet Gabriel , Socea Bogdan , Apostol Theodora Venera
Published: February 2020

In this paper we present the synthesis of the new heterocyclic compounds with 5H-dibenzo[a,d][7]annulene moiety obtained by cyclization of 2-acylhydrazinecarbothioamides (2a,b). The acylhydrazinecarbothioamides were obtained by treating 2-(5H-dibenzo[a,d][7]annulen-5-yl)acetohydrazide (1) with 2,5-difluorophenyl or 3-bromophenyl isothiocyanates. 2-Amino-1,3,4-oxadiazoles (3a,b) were synthesized by cyclization of 2- acylhydrazinecarbothioamides in the presence of mercury oxide. The new 1,2,4-triazole-3-thioles (4a,b) were synthesized by cyclization, in alkaline media, of the corresponding acylhydrazinecarbothioamide. The structures of the new compounds synthesized were investigated by 1H-NMR, 13C-NMR, IR and elemental analysis.
Municipal Wastewater Treatment, Ecological Component of Strategic Synergy in the Black Sea Basin
Authors: Woinaroschy Alexandru , Singureanu Cristina
Published: February 2020

The Black Sea is one of the world�s most polluted seas. Chemicals such as oil, fertilizers, insecticides and herbicides which do not decompose in the ground, enter the Black Sea, which becomes a huge repository for these collections mixed fertilizers and poisons. Domestic wastewater significantly contributes to pollution with organic matter and nutrients, and may be responsible for the spreading of diseases. A major component of the synergistic strategies for the environmental protection of the Black Sea consists the proper wastewater treatment. It is necessary to limit the amounts of nutrients such as ammonia, which may be discharged in treated effluents, to prevent excessive growths of algae and aquatic plants that can cause serious harm or even destroy the aesthetic value of water. In the frame of a case study, on the base of Bardenpho process, the simulation of remediation the increasing of ammonia concentration in municipal wastewater effluents was done using the SuperPro Designer v8.5 simulator. The reducing of ammonia concentrations in effluent was realized by modifications of several operating process parameters and the ammonia concentrations in effluent were decreased below the maximum legal admitted concentration. Also it was investigated the effect of the recirculated flow rate over the effluent ammonia concentration and over the total operating cost, which is a multiobjective optimization problem.
Effect of surfactant - assisted enzymatic treatment on the structure and specific properties of wool fibre
Authors: Chican Irina , Varasteanu Dana , Oproiu Loti Cornelia , Doncea Sanda Maria , Deaconu Marian , Diaconu Ioana
Published: February 2020

The objective of this study is to perform an enzymatic treatment on wool fibers in the presence of a mild detergent containing surfactants obtained from renewable raw materials, in order to obtain a reduction in the felting of wool fibers. This treatment is designed to replace the classic wool fiber process using chlorine compounds, aggressive to people and the environment. FTIR spectroscopy has demonstrated the effect of enzymes by breaking down bonds from the functional groups characteristic of the keratin protein of wool. Determination of relaxation shrinkage and felting shrinkage allowed the selection of the samples that led to a minimal felting phenomenon.
Management of Maceration-Fermentation Technologies Regarding the Antioxidant Profiles of Some Wines from Iasi Vineyard
Authors: Ignat Gabriela , Colibaba Cintia , Costuleanu Carmen Luiza , Timofte Andreea Alexandra , Sandu Ion , Moraru Ioan , Ungureanu George , Bejinariu Costica
Published: February 2020

The vine contains, in addition to sugars and acids, important amounts of phenolic compounds that accumulate in the solid parts of grapes (clusters, seeds) and skin. The quantity and quality of phenol compounds depends on both the plant and the technology of grape processing. Their high chemical reactivity makes them participate in chemical processes, especially oxido-reduction and condensation. At the same time, these substances have antioxidant qualities that delay the oxidation of wine components. Phenolic compounds also have interesting biological properties. Recent medical research attributed to phenolic compounds from grapes, juice and wines, especially red ones, remarkable sanogenic qualities, due in particular to their antioxidant action when it comes to polyphenols, cardiovascular protection, antiviral, antihistaminic, anti-cancer, anti-inflammatory, etc.This study analyses the management of various maceration-fermentation processes and their influence on the antioxidant capacity of some wines from Iasi vineyard, but also their correlation with specific phenol compounds. The management of winemaking technologies can produce wines with high antioxidant potential, a plus for an increasingly demanding consumer.
Reducing the Risk in Controlled Drug Release by Using Tannic Acid in Liposomal Formulations
Authors: Cioca Gabriela , Agop Maricel , Popa Marcel , Bungau Simona , Butuc Irina
Published: February 2020

One of the main challenges in designing a release system is the possibility to control the release rate in order to maintain it at a constant value below a defined limit, to avoid exceeding the toxicity threshold. We propose a method of overcoming this difficulty by introducing the drug into liposomes, prior to its inclusion in the hydrogel. Furthermore, a natural cross linker (as is tannic acid) is used, instead of the toxic cross linkers commonly used, thus reducing the toxicity of the release system as a whole.
Study of Carbon Monoxide Intoxication in Fire Victimis
Authors: David Sofia , Knieling Anton , Scripcaru Calin , Diac Madalina , Sandu Ion , Bulgaru Iliescu Diana
Published: February 2020

Carbon monoxide poisoning is the leading cause of mortality and morbidity of toxic origin in the world. Its insidious and polymorphic symptomatology makes it difficult to diagnose. It occurs accidentally, because of non-supervised domestic fires, or in fire victims. In fire victims, in particular, the differential diagnosis between carbon monoxide gas poisoning, inhalation of other toxic products of combustion like cyanide, oxygen deprivation, thermal burns and shock due to burns as a cause of death is not an easy task. The authors examined 107 fire victims that were autopsied at the Forensic Medicine from Iasi, Romania, in the last 10 years (2007-2016). Most cases were males (69.16%), young (0-9 years) or older than 60 with a burned surface of 91-100% in 68.22% of cases. Blood samples from the cadavers were collected in all cases in order to analyse carboxyhaemoglobin concentration and haemolysis. Toxicological analysis revealed a carboxyhaemoglobin level of maximum 95% but the majority of cases (70.72%) had a concentration inferior to 50%. An inverse correlation was identified between carboxyhaemoglobin concentration and haemolysis, an indicator of heat dissociation. Our study proves that many fire victims may die because of carbon monoxide intoxication prior to the extent of burns at a lethal potential.
Acetylcholinesterase Inhibitors Test Confirmed Myasthenia Gravis in Psychosis Remitted by Aripiprazole
Authors: Teodorescu Andreea , Ifteni Petru , Petric Paula , Toma Sebastian , Baracan Adrian , Gavris Claudia , Balan Gheorghe G. , Poroch Vladimir , Pascu Alina Mihaela
Published: February 2020

Myasthenia gravis (MG) is a chronic autoimmune neuromuscular disease affecting the neuromuscular junction and causes weakness in the skeletal muscles. The acetylcholine receptor is usually attacked in skeletal muscles, but other components of neuromuscular junction, such as muscle-specific receptor tyrosine kinase, may be affected. MG can be life-threatening when the respiratory muscles are involved. The first symptom in about 2 out of 3 cases is the damage of the extrinsic eye muscles. The condition is treatable, so an early recognition is needed. Although there have been reports of associations between psychosis and myasthenia gravis it is unclear if psychotic symptoms in MG are an integral part of the various manifestations of this disease, or are due to another co-occurring distinct disorder. Sometimes psychotic episodes could disguise the simptoms of myastenia gravis, and delay the diagnosis.
The Benefits of Platelet-Rich Fibrin in Promoting Post-Extraction Healing
Authors: Gioga Cherana , Bechir Edwin Sever , Mola Farah Curt , Defta Carmen Liliana , Bechir Anamaria , Biris Carmen , Bunget Adina Magdalena
Published: February 2020

The aim of this study was to evaluate pain intensity and wound healing of the post-extraction sites after the use of Platelet-Rich Fibrin (PRF). 240 patients which needed difficult or multiple extractions were included in the study. The extractions were realized under local anesthesia, with piezotome and pliers. The patients were divided in two groups, the first group of 120 patients which benefited the application of PRF in the post-extraction alveoli, and the second group of 120 patients represented the control group (without application of PRF). A questionnaire was realized after a VAS analog scale and was used to quantify the post-intervention pain intensity in the postoperative four days. The assessment of the post-extraction sites healing period was performed clinically. According to the obtained results, the healing period of the post-extraction sites was shorter in the patients of first group (PRF), compared to the sites of control group patients. No postextractional healing complications occurred in the patients of first group/PRF. The study demonstrated the benefits of PRF insertion in post-intervention healing.
The Benefits of Botulinum Neurotoxin Treatment in a Multitude of Medical Conditions
Authors: Neagu Tiberiu Paul , Cocolos Ion , Cobilinschi Cristian , Tiglis Mirela , Florescu Ioan Petre , Badila Elisabeta , Sinescu Ruxandra Diana
Published: February 2020

Botulinum neurotoxins (BoNTs) are responsible for botulism in humans and vertebrates, being one of the six most catastrophic potential bioterrorism agents. This are ~150 kDa proteins, assembled as a ~50 kDa light chain (LC) and a ~100 kDa heavy chain (HC). The LC acts like a zinc metalloproteinase that cleaves three proteins in neurons, members of the SNARE (Soluble N-ethylmaleimide sensitive fusion attachment protein receptors) family: VAMP (vesicle-associated membrane protein) / synaptobrevin, SNAP-25 (synaptosomal-associated protein 25) and syntaxin. After cleavage of any of this proteins, neurotransmission is blocked and flaccid paralysis of the muscle is installed. This extraordinary restricted tropism for the cholinergic presynaptic membrane makes this drug unique regarding its toxicity, pharmacological and therapeutic use. Taking into consideration the potential of this substance, this paper aims to summarize the most relevant data regarding the mechanism of actions and its main clinical applications, in order to improve medical practice. Therefore, we presented the mechanism of action in order to understand its usage in different pathologies, such as dystonias, spasticity, nephrologic and urologic conditions, cosmetic use, depression, gastroenterologic and proctologic diseases, dermatologic conditions, pathologies specific to plastic surgery and also the role of BoNT therapy in pain management. It is well documented in the literature that important discoveries have been made through recent experimental and clinical studies. Even so, there is still much to learn about the therapeutic action of this drug in terms of molecular and pathophysiological mechanisms, in order to benefit of the whole healing potential of this amazing toxin.
Immuno-biological Assessments of Temporomandibular Joint Disease in Patients with Immune-mediated Rheumatic Conditions. A cross sectional study of 273 cases
Authors: Iordache Cristina , Vascu Bogdan , Ancuta Eugen , Chirieac Rodica , Pomirleanu Cristina , Ancuta Codrina
Published: February 2020

Temporomandibular joint (TMJ) is commonly involved in various immune-mediated rheumatic disorders accounting for significant disability and impaired quality of life. The aim of our study was to assess inflammatory and immune parameters in patients with TMJ arthritis related to rheumatoid arthritis (RA), juvenile idiopathic arthritis (JIA), ankylosing spondylitis (AS) and psoriatic arthritis (PsA) and to identify potential relation with severity and dysfunction of TMJ pathology. We performed a cross-sectional study in a cohort of 433 consecutive RA, 32 JIA, 258 AS, and 103 PsA. Only patients presenting with clinically significant TMJ involvement (273) related to their rheumatic condition were included in the final analysis. TMJ involvement is traditionally described in chronic inflammatory rheumatic disorders, particularly in patients with higher levels of inflammation as detected in rheumatoid arthritis and psoriatic arthritis. Disease activity and severity, as well as biological and positive serological assessments (rheumatoid factor, anti-cyclic citrullinated peptide, IL-1) remain significant determinants of the severity of TMJ arthritis.
Glycemic Control and Interleukin-1beta in Periodontal Patients with Type 1 and 2 Diabetes in a Group of Subjects in South-West Romania
Authors: Popescu Dora , Gheorghe Dorin , Boldeanu Virgil , Rica Ana Maria
Published: February 2020

Interleukin-1b is an important mediator of the inflammatory response and is involved in the pathophysiology of periodontitis. The purpose of the study was to investigate the influence of periodontal disease and glycemic control on biomarker levels in crevicular fluid. Patients with aggressive periodontitis were characterized by a higher ratio of IL-1b compared to patients with chronic periodontitis.