bluuebunny/crossref_metadata_2025 huggingface dataset:
Functional Properties and Preservative Effect of P-Hydroxybenzoic Acid Grafted Chitosan Films on Fresh-Cut Jackfruit
Authors: Jiang Zhiguo , Wang Jiaolong , Xiang Dong , Zhang Zhengke
Published: May 2022

In the present study, p-hydroxybenzoic acid-grafted chitosan (PA-g-CS) conjugates with different grafting degrees were synthesized by a free radical-regulated grafting approach. The conjugates were further developed into films by casting, and their characteristics and preservative effects on fresh-cut jackfruit were evaluated. Compared to the CS film, the PA-g-CS film showed comprehensive performance improvements, including enhancements of water solubility, anti-ultraviolet capacity, antioxidation, and antibacterial activity. Moreover, compared with CS film, some appreciable and favorable changes of physical properties were observed in the PA-g-CS films, which included water vapor permeability, oxygen permeability, surface morphology, moisture content, and mechanical intensity. Furthermore, compared to CS alone, the application of PA-g-CS films to fresh-cut jackfruit exerted a beneficial effect on the quality of products, as indicated by the inhibition of weight loss, softening, and membrane damage, the maintenance of soluble solids and ascorbic acids contents, as well as a reduced bacterial count and a higher sensory score. Among these PA-g-CS films, the best preservation effect was achieved with the highest degree of grafting (PA-g-CS III). The results suggested that the PA-g-CS film has the potential to be explored as a new type of packaging material for the preservation of fresh-cut fruits and vegetables.
Comprehensive Evaluation of Probiotic Property, Hypoglycemic Ability and Antioxidant Activity of Lactic Acid Bacteria
Authors: Wang Hongyu , Li Liang
Published: May 2022

Taking lactic acid bacteria is an important strategy to alleviate or prevent diabetes, but the candidate strains with good genetic stability and excellent functions still need to be supplemented. In this study, the hypoglycemic ability (α-amylase, α-glucosidase and dipeptidyl peptidase 4), probiotic property and antioxidant activity of lactic acid bacteria were comprehensively evaluated by a principal component analysis (PCA) and analytic hierarchy process (AHP). The results showed that Lactobacillus paracasei(L. paracasei) had a higher survival rate (82.78%) in gastric juice and good tolerance to bile salt, and can be colonized in HT-29 cells. L. paracasei had a remarkable inhibitive activity of α-amylase (82.21%), α-glucosidase (84.29%) and dipeptidyl peptidase 4 (42.51%). L. paracasei had better scavenging activity of free radicals, total antioxidant activity (FRAP) and superoxide dismutase activity. According to the scores of the PCA, L. paracasei had the best hypoglycemic ability, and Lactococcus lactis (L. lactis) had the highest probiotic property. According to AHP, L. paracasei was the best potential hypoglycemic probiotic; furthermore, L. lactis showed the highest comprehensive performance except Lactobacillus. All lactic acid bacteria in this test had good safety. L. paracasei is expected to become a new potential hypoglycemic strain.
Impact of COVID-19 Vaccination Status and Confidence on Dietary Practices among Chinese Residents
Authors: Li Zhongyu , Ma Yidi , Huo Shanshan , Ke Yalei , Zhao Ai
Published: May 2022

Healthy diets promote immune functions and have been shown to reduce COVID-19 severity. In 2021, COVID-19 vaccines have become available to the general public. However, whether vaccination status could affect individual and populational health behaviors is unknown. This study aimed to investigate the impacts of vaccination status and confidence on dietary practices. An online survey was conducted in August 2021. We collected data on dietary intake, diversity and behaviors, vaccination status and confidence and socio-demographic characteristics. Among the 5107 responses received, a total of 4873 study participants were included in the final analysis. Most of our participants aged between 18 and 45 years and 82% of them were fully vaccinated against COVID-19. Household level dietary diversity was found to be higher among people who were fully vaccinated (β = 0.321, 95%CI: 0.024 to 0.618) or who were more confident in the protectiveness of the vaccine (β for tertile 3 comparing with lowest tertile = 0.544, 95%CI: 0.407, 0.682). Vaccination promoted the intake of seafood, but it was also positively associated with the consumption of sugar, preserved, fried and barbequed foods and reduced vegetable intake. Higher vaccination confidence was associated with increased consumption of seafood, bean, fruits and vegetables and reduced fat intake. Changes in dietary behaviors compared with early 2021 (when vaccination was not common) were observed and differed by vaccination status and confidence level. Conclusion: COVID-19 vaccination status and confidence had varied, and possibly negative, impacts on dietary intake and behaviors. Our results suggest that vaccination status and confidence might be significant influencing factors affecting people’s health behaviors and highlight that healthy eating should be consistently promoted to prevent poor dietary practices during global health crisis.
Synthesis of pH-Sensitive Cross-Linked Basil Seed Gum/Acrylic Acid Hydrogels by Free Radical Copolymerization Technique for Sustained Delivery of Captopril
Authors: Ghumman Shazia Akram , Noreen Sobia , Hameed Huma , Elsherif Mervat A. , Shabbir Ramla , Rana Mavra , Junaid Kashaf , Bukhari Syed Nasir Abbas
Published: May 2022

The pH-sensitive polymeric matrix of basil seed gum (BSG), with two different monomers, such as acrylic acid (AA) and N, N-Methylene-bis-acrylamide (MBA), was selected to use in hydrogels preparation through a free radical copolymerization technique using potassium per sulfate (KPS) as a cross linker. BSG, AA and MBA were used in multiple ratios to investigate the polymer, monomer and initiator effects on swelling properties and release pattern of captopril. Characterization of formulated hydrogels was done by FTIR, DSC/TGA, XRD and SEM techniques to confirm the stability. The hydrogels were subjected to a variety of tests, including dynamic swelling investigations, drug loading, in vitro drug release, sol–gel analyses and rheological studies. FTIR analysis confirmed that after the polymeric reaction of BSG with the AA monomer, AA chains grafted onto the backbone of BSG. The SEM micrographs illustrated an irregular, rough, and porous form of surface. Gel content was increased by increasing the contents of polymeric gum (BSG) with monomers (AA and MBA). Acidic and basic pH effects highlighted the difference between the swelling properties with BSG and AA on increasing concentration. Kinetic modelling suggested that Korsmeyer Peppas model release pattern was followed by the drug with the non-Fickian diffusion mechanism.
Joint Effects of Cigarette Smoking and Green Tea Consumption with miR-29b and DNMT3B mRNA Expression in the Development of Lung Cancer
Authors: Huang Chia-Chen , Lai Chung-Yu , Lin I-Hsin , Tsai Chin-Hung , Tsai Shi-Mei , Lam Kit-Lai , Wang Jiun-Yao , Chen Chun-Chieh , Wong Ruey-Hong
Published: May 2022

In tumor development, increased expression of DNA methyltransferase (DNMT) has been observed. In particular, cigarette smoke and tea polyphenols may influence DNMT3B mRNA expression by regulating microRNA (miR)-29b expression. Herein, we designed a case–control study to evaluate the joint effects of smoking and green tea consumption, with miR-29b and DNMT3B mRNA expression, in lung cancer development. A total of 132 lung cancer patients and 132 healthy controls were recruited to measure miR-29b and DNMT3B mRNA expression in whole blood. Results revealed that lung cancer patients had lower miR-29b expression (57.2 vs. 81.6; p = 0.02) and higher DNMT3B mRNA expression (37.2 vs. 25.8; p < 0.001) than healthy controls. Compared to non-smokers with both higher miR-29b and lower DNMT3B mRNA expression, smokers with both low miR-29b and higher DNMT3B mRNA expression had an elevated risk of lung cancer development (OR 5.12, 95% CI 2.64–9.91). Interactions of smoking with miR-29b or DNMT3B mRNA expression in lung cancer were significant. Interaction of green tea consumption with miR-29b expression and DNMT3B mRNA expression in lung cancer was also significant. Our study suggests that smokers and green tea nondrinkers with lower miR-29b expression and higher DNMT3B mRNA expression are more susceptible to lung cancer development.
Rare Variant Analysis and Molecular Dynamics Simulation in Alzheimer’s Disease Identifies Exonic Variants in FLG
Authors: Xiong Weixue , Cai Jiahui , Li Ruijia , Wen Canhong , Tan Haizhu ,   on behalf of the Alzheimer’s Disease Neuroimaging Initiative (ADNI) Database
Published: May 2022

Background: Although an increasing number of common variants contributing to Alzheimer’s disease (AD) are uncovered by genome-wide association studies, they can only explain less than half of the heritability of AD. Rare variant association studies (RVAS) has become an increasingly important area to explain the risk or trait variability of AD. Method: To investigate the potential rare variants that cause AD, we screened 70,209 rare variants from two cohorts of a 175 AD cohort and a 214 cognitively normal cohort from the Alzheimer’s Disease Neuroimaging Initiative database. MIRARE, a novel RVAS method, was performed on 232 non-synonymous variants selected by ANNOVAR annotation. Molecular docking and molecular dynamics (MD) simulation were adopted to verify the interaction between the chosen functional variants and BACE1. Results: MIRAGE analysis revealed significant associations between AD and six potential pathogenic genes, including PREX2, FLG, DHX16, NID2, ZnF585B and ZnF875. Only interactions between FLG (including wild type and rs3120654(SER742TYR)) and BACE1 were verified by molecular docking and MD simulation. The interaction of FLG(SER742TYR) with BACE1 was greater than that of wildtype FLG with BACE1. Conclusions: According to the literature search, bio-informatics analysis, and molecular docking and MD simulation, we find non-synonymous rare variants in six genes, especially FLG(rs3120654), that may play key roles in AD.
Sequence Analysis of the Complete Mitochondrial Genome of a Medicinal Plant, Vitex rotundifolia Linnaeus f. (Lamiales: Lamiaceae)
Authors: Yu Xiaoli , Duan Zhonggang , Wang Yanjun , Zhang Qingxin , Li Wei
Published: May 2022

In the present study, we depicted the complete mitochondrial genome of a valuable medicinal plant, Vitex rotundifolia. The mitochondrial genome of V. rotundifolia, mapped as a circular molecule, spanned 380,980 bp in length and had a GC content of 45.54%. The complete genome contained 38 protein-coding genes, 19 transfer RNAs (tRNAs), and 3 ribosomal RNAs (rRNAs). We found that there were only 38.73% (147.54 kb), 36.28% (138.23 kb), and 52.22% (198.96 kb) of the homologous sequences in the mitochondrial genome of V. rotundifolia, as compared with the mitochondrial genomes of Scutellaria tsinyunensis, Boea hygrometrica, and Erythranthe lutea, respectively. A multipartite structure mediated by the homologous recombinations of the three direct repeats was found in the V. rotundifolia mitochondrial genome. The phylogenetic tree was built based on 10 species of Lamiales, using the maximum likelihood method. Moreover, this phylogenetic analysis is the first to present the evolutionary relationship of V. rotundifolia with the other species in Lamiales, based on the complete mitochondrial genome.
Bulk RNA-Seq Analysis Reveals Differentially Expressed Genes Associated with Lateral Branch Angle in Peanut
Authors: Ahmad Naveed , Hou Lei , Ma Junjie , Zhou Ximeng , Xia Han , Wang Mingxiao , Leal-Bertioli Soraya , Zhao Shuzhen , Tian Ruizheng , Pan Jiaowen , Li Changsheng , Li Aiqin , Bertioli David , Wang Xingjun , Zhao Chuanzhi
Published: May 2022

Lateral branch angle (LBA), or branch habit, is one of the most important agronomic traits in peanut. To date, the underlying molecular mechanisms of LBA have not been elucidated in peanut. To acquire the differentially expressed genes (DEGs) related to LBA, a TI population was constructed through the hybridization of a bunch-type peanut variety Tifrunner and prostrate-type Ipadur. We report the identification of DEGs related to LBA by sequencing two RNA pools, which were composed of 45 F3 lines showing an extreme opposite bunch and prostrate phenotype. We propose to name this approach Bulk RNA-sequencing (BR-seq) as applied to several plant species. Through BR-seq analysis, a total of 3083 differentially expressed genes (DEGs) were identified, including 13 gravitropism-related DEGs, 22 plant hormone-related DEGs, and 55 transcription factors-encoding DEGs. Furthermore, we also identified commonly expressed alternatively spliced (AS) transcripts, of which skipped exon (SE) and retained intron (RI) were most abundant in the prostrate and bunch-type peanut. AS isoforms between prostrate and bunch peanut highlighted important clues to further understand the post-transcriptional regulatory mechanisms of branch angle regulation. Our findings provide not only important insights into the landscape of the regulatory pathway involved in branch angle formation but also present practical information for peanut molecular breeding in the future.
Evaluation of Adjuvant Systems in Non-Surgical Peri-Implant Treatment: A Literature Review
Authors: Butera Andrea , Maiorani Carolina , Gallo Simone , Pascadopoli Maurizio , Venugopal Adith , Marya Anand , Scribante Andrea
Published: May 2022

Can the use of lasers, ozone, probiotics, glycine and/or erythritol, and chlorhexidine in combination with non-surgical peri-implant treatment have additional beneficial effects on the clinical parameters? Objectives: The non-surgical treatment of peri-implant pathologies is based on mechanical debridement to eliminate bacterial biofilm and reduce tissue inflammation; some additional therapies have been studied to achieve more detailed clinical results. Materials and methods: A literature search for publications until January 2022 was conducted. The research question is formulated following the Problem, Intervention, Comparison/Control, and Outcome. Studies investigating adjunctive therapies were included. Results: In total, 29 articles were included. Most of the studies did not show any additional benefit of these therapies in the evaluation of bleeding on probing, probing pocket depth, or plaque index; among the proposed treatments, the use of laser was the one most studied in the literature, with the achievement of a reduction of bleeding and pocket depth. More studies would be needed to assess the benefit of other therapies. Conclusions: This review showed no significant improvements in the state of health in support of mechanical debridement therapy. However, the few benefits found would deserve to be considered in new clinical studies.
Biochemical Characterization of Six Traditional Olive Cultivars: A Comparative Study
Authors: Comlekcioglu Songul , Elgudayem Farah , Nogay Gozde , Kafkas Nesibe Ebru , Ayed Rayda Ben , Ercisli Sezai , Assouguem Amine , Almeer Rafa , Najda Agnieszka
Published: May 2022

Olives are an essential ingredient in Turkish food culture. Turkey has grown to become one of the top five producers of olives and olive oils in the world. Olive trees in general are found in the coastal parts of the country. The fruits of six traditional cultivars of olive (Gemlik, Domat, Memecik, Ayvalik, Cilli, and Adana Topagi), grown in Adana, were characterized based on their fruit skin color, the amount of chlorophylls, fatty acids, antioxidant activity, and total phenolic compounds, as well as volatile compounds. The international cultivar Manzanilla, grown in the same orchards as the traditional cultivars, was also included in the study to make a comparison. Compared to the Manzanilla cultivar, Memecik showed the highest amount of total phenolic content and antioxidant activity with the highest level of lightness, blueness/yellowness, and color intensity. Ayvalik presented the highest level of greenness/redness. Although the highest fruit lightness and darkness were found in the Cilli cultivar, with the highest amount of chlorophyll a (2.63 mgL−1), there was more chlorophyll b in Adana Topagi fruits (3.34 mgL−1). The highest percentage of fatty acids was found in the Gemlik cultivar of 66.81%, among which oleic acid was the major component. The total aldehydes ranged between 33.43% and 50.60%. Compared to Manzanilla and the traditional cultivars, the Domat cultivar had the highest amount of hexanal of 44.42%. Adana Topagi had the highest amount of alcohols (61.34%) and acids (1.31%). Memecik registered the highest amount of ketones (17.86%) and terpenes (20.34%). Among all cultivars, Manzanilla displayed the highest amount of esters (2.30%). In this study, traditional cultivars were found to be the richest in health-promoting chemicals. Furthermore, a significant variability among the cultivars was revealed, implying that metabolic fingerprinting approaches could be used to differentiate cultivars once more research into the effects of the growing conditions and environmental factors on the chemical profiles of each cultivar is carried out.
Foliar Spraying of Salicylic Acid Enhances Growth, Yield, and Curcuminoid Biosynthesis Gene Expression as Well as Curcuminoid Accumulation in Curcuma longa
Authors: El Sherif Fadia , Alkuwayti Mayyadah Abdullah , Khattab Salah
Published: May 2022

The application of exogenously applied salicylic acid plays important roles in improving the growth, yield, and bioactive compound compositions of different plant species. Curcuma longa is a medicinal plant that is commonly used as a spice and food additive, and has antioxidant potential. In this study, an innovative strategy for enhancing active compound production was investigated by applying a natural plant growth enhancer—namely, salicylic acid (SA)—to C. longa plants. The experiment was conducted using a complete randomized block design. The effects of SA on the growth, yield, and chemical compound contents of C. longa were recorded. Our findings demonstrated that SA significantly improved C. longa growth, yield, and curcuminoid content when compared to control treatment, with SA at 10−3 M having the greatest effect. The study also indicated that the increase in the curcuminoid content was accompanied by the overexpression of the curcumin synthase 1 (CURS1), 2 (CURS2), and 3 (CURS3) genes, as well as the diketide-CoA synthase (DCS) gene, which have been implicated in the synthesis of curcuminoids.
Early Withering of Enlarged Ovules in Pollinated Fruits of Bananas (Musa spp.) Suggest Abortion after Fertilization
Authors: Waniale Allan , Mukasa Settumba B. , Tugume Arthur K. , Kubiriba Jerome , Tushemereirwe Wilberforce K. , Tumuhimbise Robooni
Published: May 2022

Sterility in edible bananas is as a result of a long history of anthropogenic-driven selection for sterile genotypes, since seed is not desirable in fruit pulp for human consumption. However, this poses a challenge to conventional genetic improvement by slowing breeding pipelines. In this study, we investigated whether pollen tubes reach all parts of the ovary, the position of fertilized ovule development in fruits, and potential seed set in selected banana genotypes. We selected four cultivars of East African Highland Cooking bananas (EAHBs), a Matooke hybrid ‘222K-1’, improved diploid ‘2905’, and wild bananas ‘Zebrina (G.F.)’ and ‘Calcutta 4’. There was evidence of pollen tubes in the distal, mid and proximal sections of the fruit, irrespective of hand position and genotype. Fertilization, as indicated by an increase in ovule size, happened along the entire length of the fruit but complete development was biased at the distal end in some genotypes. There were some differences in ovule fertilization rates between hands, with distal hands having more ovules and higher ovule fertilization rates. Ovule fertilization happens in bananas but the vast majority aborts, especially at the proximal end of the ovary. Ovule fertilization rates are generally much lower than available ovules.
Evaluation of the Soil Type Effect on the Volatile Compounds in the Habanero Pepper (Capsicum chinense Jacq.)
Authors: Oney-Montalvo Julio Enrique , López-Salas Diego , Ramírez-Rivera Emmanuel , Ramírez-Sucre Manuel Octavio , Rodríguez-Buenfil Ingrid Mayanin
Published: May 2022

The aim of this research was to evaluate the effect of soil on the concentration of the main volatile compounds in the Habanero pepper (Capsicum chinense Jacq.). Plants were cultivated in three soils named, corresponding to the Maya classification, as Chich lu’um (brown soil), Box lu’um (black soil), and K’ankab lu’um (red soil). The volatile compounds of the peppers were extracted by steam distillation, analyzed by gas chromatography, and reported on a fresh weight (FW) basis. The results indicated that the soil presented a significative effect on the concentration of the volatile compounds evaluated (1-hexanol, hexyl-3-methyl butanoate, 3,3-dimethyl-1-hexanol, cis-3-hexenyl hexanoate). The peppers cultivated in black soil exhibited the highest concentration of 1-hexanol (360.14 ± 8.57 µg g−1 FW), 3,3-dimethyl-1-hexanol (1020.61 ± 51.27 µg g−1 FW), and cis-3-hexenyl hexanoate (49.49 ± 1.55 µg g−1 FW). In contrast, the highest concentration of hexyl-3-methyl butanoate (499.93 ± 5.78 µg g−1 FW) was quantified in peppers grown in brown soil. This knowledge helps us to understand the role of the soil in the aroma of the Habanero pepper and could be used by farmers in the region (Yucatan Peninsula) to select the soil according to the desired aroma characteristics.
How Do Adolescents Use Social Networks and What Are Their Potential Dangers? A Qualitative Study of Gender Differences
Authors: de Felice Giulio , Burrai Jessica , Mari Emanuela , Paloni Fabrizio , Lausi Giulia , Giannini Anna Maria , Quaglieri Alessandro
Published: May 2022

The rapid development of software applications and the increasing use of the Internet have raised many questions about the impact of this technology on the lives of adolescents, especially on “digital natives.” The advent of social networks (SNs) restructures their relationships in various ways, affecting both adolescents’ development and mental health. The present study aims to investigate uses and dangers of SNs according to a sample of 296 (166 female and 130 male) Italian middle and high schools adolescents (age range 13–18) and build a model of how SNs can turn out to be dangerous. To achieve this, twenty-four audio-recorded focus groups of Italian male and female adolescents were investigated by a Grounded Theory approach, abstracting from the transcripts the main uses and dangers of SNs and proposing a final model for the interpretation of the whole set of categories. The results highlighted two main dangers of SNs: (a) the desperate search for popularity, and (b) the exhibition of violent or offensive behavior facilitated by the sense of protection and anonymity derived from being hidden behind a virtual account. Finally, a psychological model of how SNs can turn out to be dangerous is presented. This study could be useful in developing prevention procedures against the risks of SNs (e.g., cyberbullying, internet addiction) without demonizing the use of social media as such.
Awareness and Knowledge of HPV, HPV Vaccination, and Cervical Cancer among an Indigenous Caribbean Community
Authors: Warner Zachary Claude , Reid Brandon , Auguste Priscilla , Joseph Winnie , Kepka Deanna , Warner Echo Lyn
Published: May 2022

Caribbean women experience a cervical cancer incidence rate that is three times higher than that among their North American counterparts. In this study, we performed a needs assessment of the knowledge and awareness of HPV, HPV vaccination, and cervical cancer and receipt of cervical cancer screening among an indigenous Caribbean community. We purposively recruited individuals aged ≥18 from a community health care clinic (n = 58) to complete a 57-item structured interview including items on demographics, cancer history, knowledge and awareness of HPV, HPV vaccines, cervical cancer, and cervical cancer screening. Participants’ mean age was 47.1 years (SD: 14.4). Most were female (74.1%), were married/partnered (51.7%), had primary education (63.8%), and identified as Kalinago (72.4%). Whereas 79.5% had heard of cervical cancer, few had heard of HPV (19.6%) or the HPV vaccine (21.8%). Among those who knew someone with cancer, 90.9% had heard of the HPV vaccine, compared with only 9.1% of those who did not know anyone with cancer (p = 0.02). Access to HPV vaccination is an immediate, cost-effective cancer prevention priority for reducing the disproportionate burden of HPV-related cancers, particularly cervical cancer, in the Caribbean. We recommend culturally targeted education interventions to improve knowledge about HPV vaccination and the link between HPV and cervical cancer.
Study on Factors That Influence Human Errors: Focused on Cabin Crew
Authors: Kim Jiyoung , Yu Myoungjin , Hyun Sunghyup Sean
Published: May 2022

This study aims to reduce the possibility of human mistakes and accidents among airline cabin crew by identifying the cause of human errors by focusing on the importance of the causal relationship between human factors and human errors. According to statistical analysis, among the five human factors, physical fatigue, psychological stress, and the complacency of cabin crew had a positive impact on human errors. However, hurrying under time pressure and the distractions caused by external factors do not significantly affect human errors. Human errors have a negative impact on job crafting and mental health. This study analyzed the human factors influencing the cabin crew’s errors and revealed the importance of complacency, which was not covered in previous studies. Finally, the research implications, limitations, and future studies were discussed.
Construction of Ecological Security Pattern Based on the Importance of Ecological Protection—A Case Study of Guangxi, a Karst Region in China
Authors: Yang Yanping , Chen Jianjun , Huang Renjie , Feng Zihao , Zhou Guoqing , You Haotian , Han Xiaowen
Published: May 2022

The ecological security pattern is an important way to coordinate the contradiction between regional economic development and ecological protection and is conducive to promoting regional sustainable development. This study examines Guangxi, a karst region in China. The ecosystem service function and ecological environment sensitivity were both selected to evaluate the ecological conservation importance, and based on the results of the ecological conservation importance evaluation, suitable patches were selected as ecological sources. Meanwhile, resistance factors were selected from both natural factors and human activities to construct a comprehensive resistance surface, circuit theory was used to identify ecological corridors, ecological pinch points, and ecological barrier points, and ecological protection suggestions were then proposed. The results show that there are 50 patches of ecological sources in Guangxi, with a total area of 60,556.99 km2; 115 ecological corridors, with the longest corridor reaching 194.97 km; 301 ecological pinch points, whose spatial distribution is fragmented; and 286 ecological barrier points, most of which are concentrated in the central part of Guangxi. The results of this study provide a reference for the construction of ecological security patterns and ecological conservation in developing countries and karst areas.
Investigation of the Relationship between Lean Muscle Mass and Erythropoietin Resistance in Maintenance Haemodialysis Patients: A Cross-Sectional Study
Authors: Chiang Wen-Fang , Hsiao Po-Jen , Wu Kun-Lin , Chen Hung-Ming , Chu Chi-Ming , Chan Jenq-Shyong
Published: May 2022

Each patient undergoing maintenance haemodialysis (MHD) has a different response to erythropoiesis-stimulating agents (ESAs). Haemodilution due to fluid overload has been shown to contribute to anaemia. Body mass index (BMI) has been shown to influence ESA response in dialysis patients; however, BMI calculation does not distinguish between fat and lean tissue. The association between lean muscle mass and erythropoietin hyporesponsiveness is still not well-known among MHD patients. We designed a cross-sectional study and used bioimpedance spectroscopy (BIS) to analyse the relationship between body composition, haemoglobin level, and erythropoietin resistance index (ERI) in MHD patients. Seventy-seven patients were enrolled in the study group. Compared with patients with haemoglobin ≥ 10 g/dL, those with haemoglobin < 10 g/dL had higher serum ferritin levels, malnutrition–inflammation scores (MIS), relative overhydration, ESA doses, and ERIs. In multivariate logistic regression, higher ferritin levels and MIS were the only predictors of lower haemoglobin levels. The ERI was significantly positively correlated with age, Kt/V, ferritin levels, and MIS and negatively correlated with albumin levels, BMI, and lean tissue index (LTI). Multivariate linear regression analysis revealed that ferritin levels, BMI, and LTI were the most important predictors of ERI. In MHD patients, using BIS to measure body composition can facilitate the development of early interventions that aim to prevent sarcopenia, support ESA responsiveness, and, consequently, improve anaemia management.
Consumer Perspectives on Anxiety Management in Australian General Practice
Authors: Parker Erin , Banfield Michelle
Published: May 2022

The aim of the current study was to explore consumer views on the management of anxiety in general practice, which is often the first service from which a consumer seeks professional support. We used a mixed methods survey to explore three broad research questions: (1) what are consumer experiences of anxiety management in general practice, (2) what do consumers prioritise when considering treatment for anxiety and what are their preferences for type of treatment, and (3) how do consumers think care for anxiety could be improved? Consumers reported generally positive views of their GP when seeking help for anxiety, though they had mixed experiences of the approach taken to treatment. Consumers noted that they prioritise effective treatment above other factors and are less concerned with how quickly their treatment works. A preference for psychological intervention or combined treatment with medication was apparent. Consumers noted that key areas for improving care for anxiety were improving access and funding for psychological treatments, increasing community knowledge about anxiety, and reducing stigma.
Somatic Development Disorders in Children and Adolescents Affected by Syndromes and Diseases Associated with Neurodysfunction and Hydrocephalus Treated/Untreated Surgically
Authors: Perenc Lidia , Guzik Agnieszka , Podgórska-Bednarz Justyna , Drużbicki Mariusz
Published: May 2022

Background: This study was conducted to evaluate the co-occurrence of hydrocephalus treated/untreated surgically and congenital nervous system disorders or neurological syndromes with symptoms visible since childhood, and with somatic development disorders, based on significant data obtained during admission to a neurological rehabilitation unit for children and adolescents. Methods: The study applied a retrospective analysis of data collected during hospitalization of 327 children and adolescents, aged 4–18 years, all presenting congenital disorders of the nervous system and/or neurological syndromes associated with at least one neurodysfunction that existed from early childhood. To allow the identification of individuals with somatic development disorders in the group of children and adolescents with hydrocephalus treated/untreated surgically, the adopted criteria considered the z-score values for body height, body weight, head circumference, body mass index, and head circumference index. Results: Treated/untreated hydrocephalus was observed in the study group at the rates of 8% and 0.9%, respectively. Among 239 patients with cerebral palsy, 9 (3.8%) had surgically treated hydrocephalus, 17 (70.8%) of 24 patients with neural tube defects also had hydrocephalus treated with surgery, and 3 (12.5%) of 24 patients with neural tube defects had untreated hydrocephalus. This medical condition was a more frequent comorbidity in subjects with neural tube defects compared with those with cerebral palsy (p < 0.001). Subjects with untreated hydrocephalus most frequently presented macrocephaly (p < 0.001), including absolute macrocephaly (p = 0.001), and with tall stature (p = 0.007). Excessive body mass co-occurred more frequently with surgically untreated hydrocephalus, but the relationship was not statistically significant (p = 0.098). Conclusions: Surgically treated hydrocephalus occurred in patients with cerebral palsy and neural tube defects, and untreated hydrocephalus was present only in patients with neural tube defects. Untreated hydrocephalus negatively changed the course of individual development in the studied group of children, in contrast to surgically treated hydrocephalus.
Concentration, Health Risk, and Hydrological Forcing of Heavy Metals in Surface Water Following Water-Sediment Regulation of the Xiaolangdi Dam in the Yellow River
Authors: Zhao Qinghe , Ding Shengyan , Geng Zihan , Lu Xunling , Hong Zhendong , Liu Yi , Yu Jinhai
Published: May 2022

Water and sediment regulation aimed at aquatic ecosystems and preserving reservoir capacity to minimize the negative consequences of dams can fundamentally change the distribution of heavy metals (HMs) in the reservoir and downstream reaches. However, the effects of water and sediment regulation on variation in HMs are still poorly understood. In this study, the variations in concentration, contamination, human health risk, potential sources, and influencing factors of the metalloid As and HMs (Cr, Hg, Ni, Pb, and Zn) in surface water in the reservoir and the downstream reach of the Xiaolangdi Dam (XLD) following the operation of the water-sediment regulation scheme (WSRS) were determined. These results indicate that HM concentrations in the two post-WSRS seasons were much lower than the water quality standards, but were significantly increased over time due to the trapping effects of the XLD (p < 0.05, except for Zn). However, As concentration in the reservoir was significantly lower than that observed in downstream reaches, likely due to anthropogenic input from agricultural activities. Meanwhile, HM concentrations varied with distance to the dam, which displayed a distinct accumulation closer to the dam in the post-WSRS II season. The contamination of HMs, the carcinogenic risk of exposure to As, and the noncarcinogenic risks associated with exposure to Hg, Ni, Pb, and Zn via the direct ingestion pathway of drinking water were all within acceptable levels following the WSRS, but increased over time. The carcinogenic risk of Cr in the post-WSRS II season was at an unacceptably high level, particularly at sites near the dam. Hydrological characteristics (water level and flow rate) were the dominant factors in determining the distribution of HMs. These results can provide new insight for a better understanding of the variations in HMs following the water and sediment regulation practices, and guide future management in regulating the trapping effects of dams.
Assessment of Acceptability and Initial Effectiveness of a Unified Protocol Prevention Program to Train Emotional Regulation Skills in Female Nursing Professionals during the COVID-19 Pandemic
Authors: Ferreres-Galán Vanesa , Navarro-Haro María Vicenta , Peris-Baquero Óscar , Guillén-Marín Silvia , de Luna-Hermoso Jordi , Osma Jorge
Published: May 2022

Healthcare professionals, especially women, have shown increases in anxious-depressive symptoms as a consequence of the COVID-19 pandemic. The aim of this pilot study was to evaluate the acceptability and preliminary effectiveness of a Unified Protocol (UP) prevention program to provide emotional regulation skills to cope with stressful situations. The sample consisted of 27 nursing professionals (100% women; mean age: 45.67; SD = 7.71) working in a Spanish public hospital during COVID-19, who were randomized to an immediate treatment group (ITG, n = 13) or to a delayed treatment group (DTG, n = 14, which served as the waiting list control group and received the program 5 weeks after the ITG had received it). The program consisted of five-weekly, two-hour, UP-based group sessions. Variables related to emotional symptomatology, emotional regulation, personality, burnout, and perceived quality of life were evaluated at the following time points: pre- and post-intervention and at 1-, 3-, and 6-month follow-ups. Statistically significant between-group differences showed lower emotional exhaustion and personal accomplishment in favor of the ITG after the intervention. Regarding the effect over time for all participants who received the UP (n = 27), statistically significant reductions were observed in neuroticism, personal accomplishment, and subjective distress caused by traumatic events (−0.23 ≤ d ≤ −0.73). A statistically significant interaction “Time*Condition” was found in anxiety, with increases in the DTG. Participants showed high satisfaction with the UP. These findings show good acceptability and preliminary effectiveness of the UP to reduce the emotional impact of the pandemic in female nursing workers.
Caregiver Characteristics of Adults with Acute Traumatic Brain Injury in the United States and Latin America
Authors: Juengst Shannon B. , Perrin Paul B. , Klyce Daniel W. , O’Neil-Pirozzi Therese M. , Herrera Susan , Wright Brittany , Lengenfelder Jean , Lercher Kirk , Callender Librada , Arango-Lasprilla Juan Carlos
Published: May 2022

Objectives: To compare characteristics of caregivers of adults with acute traumatic brain injury (TBI) in the U.S. and Latin America (Mexico and Colombia). Design: Secondary data analysis of two cohorts. Cohort 1: English-speaking caregivers of adults with TBI in the U.S. (n = 80). Cohort 2: Spanish-speaking caregivers of adults with TBI in Mexico or Colombia (n = 109). Results: Similarities between the U.S. and Latin American caregiver groups, respectively, were: predominantly women (81.3%, 81.7%, respectively); spouses/domestic partners (45%, 31.2%); and motor vehicle accident (41.5%, 48.6%) followed by fall etiologies (40%, 21.1%). Differences between U.S. and Latin American caregivers were: age (49.5 years, 41.5 years, p < 0.001); employment status ((Χ52 = 59.63, p < 0.001), full-time employment (63.7%, 25.7%), homemaker (2.5%, 31.2%), and retired (17.5%, 1.8%)); violence-related etiology (2.5%, 15.6%); and severity of depressive symptoms (M = 7.9, SD = 5.8; M = 5.8, SD = 5.7; p = 0.014). Conclusions: TBI caregivers in the U.S. were older and employed full-time or retired more often than those in Latin America. Violence-related etiology was nearly five times more common in Latin America, raising concerns for potential implications of post-traumatic stress and family adjustment after injury. Although both groups likely could use mental health support, this was particularly true of the U.S. cohort, maybe due to differential demographics, mechanisms of injury, or family and community support.
Differential Profile of Specialist Aggressor versus Generalist Aggressor in Child-to-Parent Violence
Authors: Navas-Martínez María J. , Cano-Lozano M. Carmen
Published: May 2022

Research on violence in general highlights the need to differentiate between those aggressors who only show specialized violence in the family context and those who also show generalized violence in other contexts outside the family. However, in the phenomenon of child-to-parent violence (CPV), the distinctive characteristics of this profile have not been yet analyzed. The aim of this study was to identify the typology of specialist aggressor versus the typology of generalist aggressor and examine whether they differ in their characteristics. A total of 1559 CPV aggressors participated, with ages between 12 and 18 years, of whom 22.4% exerted violence only towards parents (specialist aggressors) and 77.6% also exerted violence towards peers (generalist aggressors). The results show that specialized violence and generalized violence seem to follow different patterns according to age. The generalists were characterized by a more negative profile than the specialists. Specifically, the former showed more CPV and for more reasons, both reactive and proactive. Regarding individual characteristics, they obtained lower levels of emotional intelligence and resilience. Concerning family characteristics, they presented higher levels of insecure parental attachment and parental violence (direct and observed). The predictive variables retained in the regression model represented approximately 16.4% of the variation in the type of aggressor. This study supports the classification based on the specificity versus generality of violence, as it was found that specialist and generalist CPV aggressors differ significantly in their characteristics. It is considered that the findings could help to identify the differential mechanisms through which both types of aggressors have developed CPV. Further analysis of this profile can be of great use for the design of intervention and prevention programs adapted to the needs of each typology.
Perfluoroalkyl Substances (PFASs) in Rivers and Drinking Waters from Qingdao, China
Authors: Lu Guohui , Shao Pengwei , Zheng Yu , Yang Yongliang , Gai Nan
Published: May 2022

Perfluoroalkyl substances (PFASs) in rivers; drinking water sources (reservoirs and groundwater); and various types of drinking waters (tap waters, barreled pure waters, and bottled mineral waters) in Qingdao, Eastern China were quantified by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The total concentrations of PFASs (ΣPFASs) in the river waters ranged from 28.3 to 292.2 ng/L, averaging 108 ± 70.7 ng/L. PFBS was the most abundant compound, with a maximum concentration of 256.8 ng/L, followed by PFOA (maximum concentration: 72.4 ng/L) and PFBA (maximum concentration: 41.6 ng/L). High levels of PFASs were found in rivers in the suburban and rural areas. The estimated annual mass loading of the total PFASs to Jiaozhou Bay (JZB) was 5.9 tons. The PFASs in the drinking water reservoirs were relatively low. The ΣPFASs in the tap water ranged from 20.5 ng/L to 29.9 ng/L. Differences in the PFAS levels and composition profiles were found among barreled water at different market sites and for different brands of mineral water products. The sequence of the contamination levels of the waters related to drinking water was reservoir water > tap water > barrel water > groundwater > bottled mineral water. The PFASs in drinking water may not pose a serious risk to the drinking water consumers of Qingdao City.
The Effect of Capacitive and Resistive Electric Transfer Intervention on Delayed-Onset Muscle Soreness Induced by Eccentric Exercise
Authors: Nakamura Masatoshi , Sato Shigeru , Kiyono Ryosuke , Yahata Kaoru , Yoshida Riku , Kasahara Kazuki , Konrad Andreas
Published: May 2022

This study aimed to investigate the acute effect of capacitive and resistive electric transfer (CRet) intervention on eccentrically damaged muscle. A total of 28 healthy and sedentary male volunteers were randomly allocated to either CRet intervention or control groups. The participants performed a bout of eccentric exercise of the knee extensors with the dominant leg and received 30 min of CRet intervention of the quadriceps 48 h after the exercise. The dependent variables for the analysis were knee flexion range of motion (ROM), muscle soreness and maximum voluntary isometric (MVC-ISO), and concentric contraction (MVC-CON) torque of the knee extensors. These were measured prior to exercise (baseline) and before and after CRet intervention (48 h after the exercise). The results showed that knee flexion ROM, muscle strength (MVC-ISO and MVC-CON), and muscle soreness significantly improved after CRet intervention. CRet intervention may improve muscle soreness and loss of muscle function in an eccentrically damaged muscle.
Comparing and Contrasting the Impacts of Macro-Level Factors on Crash Duration and Frequency
Authors: Chand Sai , Li Zhuolin , Alsultan Abdulmajeed , Dixit Vinayak V.
Published: May 2022

Road traffic crashes cause social, economic, physical and emotional losses. They also reduce operating speed and road capacity and increase delays, unreliability, and productivity losses. Previous crash duration research has concentrated on individual crashes, with the contributing elements extracted directly from the incident description and records. As a result, the explanatory variables were more regional, and the effects of broader macro-level factors were not investigated. This is in contrast to crash frequency studies, which normally collect explanatory factors at a macro-level. This study explores the impact of various factors and the consistency of their effects on vehicle crash duration and frequency at a macro-level. Along with the demographic, vehicle utilisation, environmental, and responder variables, street network features such as connectedness, density, and hierarchy were added as covariates. The dataset contains over 95,000 vehicle crash records over 4.5 years in Greater Sydney, Australia. Following a dimension reduction of independent variables, a hazard-based model was estimated for crash duration, and a Negative Binomial model was estimated for frequency. Unobserved heterogeneity was accounted for by latent class models for both duration and frequency. Income, driver experience and exposure are considered to have both positive and negative impacts on duration. Crash duration is shorter in regions with a dense road network, but crash frequency is higher. Highly connected networks, on the other hand, are associated with longer length but lower frequency.
Cigarillo Flavor and Motivation to Quit among Co-Users of Cigarillos and Cannabis: A Structural Equation Modeling Approach
Authors: Glasser Allison M. , Nemeth Julianna M. , Quisenberry Amanda J. , Shoben Abigail B. , Trapl Erika S. , Klein Elizabeth G.
Published: May 2022

Flavored cigar restrictions have the potential to benefit public health. Flavor availability facilitates cigarillo use, but it is unknown if flavor impacts patterns of co-use of cigarillos and cannabis, an increasingly prevalent behavior among young adults. Data were collected (2020–2021) in a cross-sectional online survey administered to a convenience sample of young adults who smoked cigarillos from 15 areas with high cigar use prevalence. We assessed the relationship between flavored cigarillo use and motivation to quit cannabis and cigarillo use among past 30-day co-users (N = 218), as well as several covariates (e.g., cigarillo price and flavor/cannabis policy). Flavored cigarillo perceived appeal and harm were hypothesized parallel mediators. Most co-users reported usually using flavored cigarillos (79.5%), which was not significantly associated with motivation to quit cigarillos or cannabis. Perceived cigarillo harm (β = 0.17, 95% CI = 0.00, 0.33), advertising exposure (β = 0.12, 95% CI = 0.00, 0.24), and income (among racial/ethnic minorities; β = −0.13, 95% CI = −0.25, −0.02) were significant predictors of motivation to quit cigarillos. There were no significant predictors of motivation to quit cannabis. Cigarillo flavor was not associated with motivation to quit, so findings could suggest that banning flavors in cigars may have a neutral impact on co-use with cannabis among young adults.