bluuebunny/crossref_metadata_2025 huggingface dataset:
FilmEU opportunities and challenges: Mapping a Sustainable Support Model for Practice-based Researchers, Supervisors and Examiners
Authors: Rodrigues Érica Faleiro , O´'Toole Deidre , Damásio Manuel José
Published: October 2022

This research statement complements the podcast with the same title and is construed as a stepping stone towards a sustainable support model for practice based researchers, supervisors and examiners within the FilmEU alliance and, most importantly, beyond it, opening up the debate on the opportunities and challenges facing the topic. For this task, four relevant players from the artistic research arena were interviewed: Till Ansgar Baumhauer, Nico Carpentier, Michelle Teran and Florian Cramer. FilmEU includes Lusófona University, Portugal, Baltic Film and Media School, Estonia, LUCA, Belgium, and IADT, Ireland. In order to create a model with long-term impact for practice and artistic-based research, the alliance is pursuing a common and transdisciplinary research culture on artistic research within the field of Film and Media Arts. A major hurdle identified by the alliance is the upskilling of practitioners to ensure they have the skills and knowledge necessary to perform and supervise practice-based research. Many potential candidates and supervisors are already distinguished within the worlds of practice or academia; hence, the next step will be creating a bridge between practitioners and researchers. FilmEU will have to provide the adequate technical resources and expertise to guide researchers through this transition from practitioner to practitioner-researcher and to ensure that the work created achieves a professional standard.
Ku Po Schon - Where are you going? (The way of the photo troubadour)
Authors: Hastings Jeremy
Published: October 2022

Ku Po Schon - Where are you going? (The way of the photo troubadour)
PENGARUH MOTIVASI KERJA, DISIPLIN KERJA DAN GAJI TERHADAP KINERJA GURU PEGAWAI NEGERI SIPIL SMA NEGERI COLOMADU, KARANGANYAR
Authors: Putri Dwi Mustika , Sutarno Sutarno , Sutarno Sutarno , Indriastuti Dorothea Ririn , Indriastuti Dorothea Ririn
Published: October 2022

This research was conducted on SMA Negeri Colomadu Karanganyar which is using qualittiive data and quantitative data. The data resources are secondary data and primary data. The overall population in this study were civil servant teachers at SMA Negeri Colomadu Karanganyar totaling 47 people, the sampel used in this study was taken by 47 respondents. Technical sampling in this research that is used simple census. This research informs that work motivation, work discipline, salary significantly affect performance of PNS teachers.
Aplikasi Pemesanan Tiket Bus BMA Trans Makassar berbasis Web
Authors: Saleh Muh Dasriyanto , Anraeni Siska , Mude Muh. Aliyazid
Published: October 2022

Aplikasi   pemesanan   tiket   Bus  BMA   Trans  Makassar   berbasis  web merupakan  sebuah  sistem  yang  dapat  membantu  para  calon  penumpang memesan tiket tanpa harus datang ke lokasi penjualan dan membantu para perusahaan penjual tiket dalam meningkatkan kualitas pelayanan mereka kepada para pelanggan. Teknologi web mampu memberikan informasi penting yang bermanfaat bagi calon penumpang, jadwal keberangkatan, hingga ketersediaan tempat duduk dari masing-masing perjalanan dan dapat diakses secara real time dengan mudah dalam waktu yang singkat. Hasil penelitian menunjukkan bahwa sistem yang telah berhasil dikembangkan meningkatkan efisiensi waktu bagi pelanggan yang ingin memesan tiket kapan saja.
The Effect of Siwak Wood Extract (Salvadora Persica) Solution on Oral Health: Literature Review
Authors: Arifin Arina Fathiyyah , Marwah Sry , Arfah Arni Isnaini , K Sri Irmandha , Gayatri Sri Wahyuni
Published: October 2022

Background: In terms of beliefs, culture and values or life practices, Islam encourages its adherents to keep themselves and their environment clean, including guidance on maintaining oral hygiene using miswak.. Siwak extract has an antibacterial effect, is effective against bacteria that play a role in the formation of dental plaque thus, with a decrease in plaque bacteria, the salivary pH will also increase One of the contents of miswak (Saldavora persica) hich is useful for preventing dental plaque is trimethylamine (TMA). In addition, it is also potential as an antibacterial.(3) (4) The World Health Organization (1987) recommends the use of miswak as an effective tool for oral health, with the mechanical action of soft-wood fibers and the therapeutics action of its chemical content.

Content: it is necessary to conduct a Literature Review study to determine the effect of a solution of siwak wood extract (Salvadora Persica) on oral health. The feature of this research is we want to know with siwak extract can be maintained acid-base balance in salivary pH which can kill some bacteria in the mouth. The purpose of this study was to determine the effect of a solution of siwak wood extract on oral health. Literature Review with Systematic review design.

Conclusion: The effect of a solution of siwak wood extract on oral health, concluded that siwak extract can affect salivary pH, production of salivary secretions, reduce plaque scores and inhibit the microorganisms development in  the oral cavity
PERUBAHAN PARADIGMA PENATAAN REGULASI DI INDONESIA
Authors: Wijaya Viona
Published: October 2022

Paradigma penataan regulasi Indonesia selama ini masih berfokus pada penataan peraturan perundang-undangan dengan negara sebagai regulator utama. Paradigma ini sebetulnya memiliki banyak kelemahan yang tidak kompatibel dengan tujuan yang hendak dicapai penataan regulasi yakni menciptakan regulasi yang fleksibel, efektif, dan efisien. Teori-teori regulasi kontemporer seperti Responsive Regulation dan Regulatory State dapat memberikan masukan yang kaya untuk perubahan paradigma penataan regulasi di Indonesia. Penelitian ini menggunakan paradigma konstruktivisme dan metode penelitian sosio-legal dengan pendekatan kualitatif. Hasil penelitian menunjukkan bahwa untuk mewujudkan penataan regulasi yang berkelanjutan dan menyeluruh, diperlukan perubahan paradigma yang mendasar terhadap regulasi dan peran negara sebagai regulator. Penataan regulasi di masa depan tidak hanya meliputi peraturan perundang-undangan tetapi juga terhadap bentuk regulasi lainnya yang eksis di tengah masyarakat. Sementara itu, peran negara sebagai regulator harus berubah dari cara pikir sentralisasi dan pendekatan command-and-control menjadi manajemen jejaring regulasi yang demokratis, menyeluruh, dan kolaboratif.  
Penafsiran Pembentuk Undang-Undang Membentuk Kebijakan Hukum Terbuka Presidential Threshold Dalam Undang-Undang Pemilihan Umum Yang Bersumber Dari Putusan Mahkamah Konstitusi
Authors: Saifulloh Putra Perdana Ahmad
Published: October 2022

Penafsiran Pembentuk Undang-Undang dalam Menafsirkan Kebijakan Hukum Terbuka Presidential Threshold dalam Undang-Undang Pemilihan Umum yang Bersumber dari Putusan Mahkamh Konstitusi, yaitu karena Presidential Threshold adalah 1). Kebijakan Hukum Terbuka yang dimana kewenangan mutlak Pembentuk Undang-Undang untuk mengatur lebih lanjut suatu pengaturan dalam Undang-Undang. Hal-hal yang termasuk dalam kebijakan hukum terbuka dalam Undang-Undang lazimnya tidak boleh dibatalkan Mahkamah Konstitusi kalau tidak secara nyata bertentangan dengan konstitusi. 2). Kebijakan ini memberikan keadilan kepada Partai Politik berdasarkan suara yang diperoleh dari Pemilihan Umum sebelumnya. 3). Memperkuat Sistem Presidensiil. Untuk itu dibutuhkan Formula Konstitusional Agar Pembentuk Undang-Undang Mematuhi Putusan Mahkamah Konstitusi Yang Menyatakan Kebijakan Dalam Undang-Undang Bersifat Kebijakan Hukum Terbuka, yaitu: 1). Membangun Dialog Konstitusional dan Tindakan Kolaboratif antara Mahkamah Konstitusi dengan Pembentuk Undang-Undang; 2). Merevisi Undang-Undang Mahkamah Konstitusi dan Undang-Undang Pembentukan Peraturan Perundang-Undangan. Penelitian ini menggunakan metode penelitian hukum normatif dengan pendekatan perundang-undangan, perbandingan, kasus, dan konseptual.

Kata Kunci: Kebijakan Hukum Terbuka, Presidential Threshold, Putusan Mahkamah Konstitusi
INTERNAL TOURISM DEVELOPMENT STRATEGY INCREASING REGIONAL ORIGINAL INCOME (PAD) IN JAYAPURA CITY PAPUA PROVINCE
Authors: Mebri Fredrick Hendrick , Suradinata Ermaya , Kusworo Kusworo
Published: October 2022

ABSTRAK. Pendapatan Asli Daerah   (PAD)  merupakan gambaran potensi keuangan daerah, dimana terdapat berbagai sektor yang dapat dikembangkan untuk meningkatkan Pendapatan asli daerah salah satunya adalah sektor pariwisata, sehingga keberadaan pariwisata diharapkan mampu menjadi salah satu penyumbang bagi pendapatan asli daerah, namun sektor pariwisata di Kota Jayapura belum dikelola dan dimanfaatkan secara optimal, dalam kenyataannya banyaknya aset wisata di Kota Jayapura yang masih dikuasai oleh pemilik hak ulayat dengan melakukan pungutan liar, kurangnya hubungan kerjasama dengan dinas-dinas terkait dalam membangun dan mengembangkan objek wisata, dan kurangnya partisipasi masyarakat dalam mengembangkan objek wisata. Berdasarkan hal tersebut maka penulis tertarik untuk melakukan penelitian dengan judul; "Strategi Pengembangan Pariwisata Dalam Meningkatkan Pendapatan Asli Daerah (PAD) di Kota Jayapura Provinsi Papua. Penelitian ini menggunakan metode kualitatif dengan pendekatan deskriptif untuk menggambarkan tentang keadaan di lapangan yang kemudian diteliti untuk menemukan pemecahan masalah yang bersifat umum. Data yang dihimpun untuk penelitian ini adalah data yang terkait dengan analisis  ASOCA terhadap strategi pengembangan pariwisata dalam meningkatkan pendapatan asli daerah (PAD) serta implikasinya dalam menentukan strategi yang akan dilakukan oleh Dinas Pariwisata Kota Jayapura Provinsi Papua. Penelitian ini dibuat untuk mengetahui dan memaparkan sejauh mana strategi Dinas Pariwisata dalam pengembangan pariwisata untuk meningkatkan pendapatan asli daerah di Kota Jayapura seiring dengan fenomena yang menggambarkan hambatan yang dihadapi dan upaya dalam mengatasi hambatan.

Kata kunci: Strategi, Pendapatan Asli Daerah (Pad), Pariwisata.
Modern tectonic movements as a predetermining factor in the formation and preservation of gas accumulations (on the example of the Kovyktinskoye field)
Authors: Lapin P. S.
Published: October 2022

One of the possible methods for evaluating the activation of deep-immersed geological bodies in the latest tectonic movements is described, which is based on the discovery of a relief-forming process as a result of the action of endogenous and exogenous forces. In the process of modeling, the influence of the activation of deep geological bodies on the post-salt and pre-salt complexes within the Kovyktinskoye field was established. For the post-salt complex, an area of slight activation of tectonic movements within the promising part of the Nizhneangarskaya suite was revealed. For the pre-salt complex, the tectonic nature of the boundaries for the most productive part of the deposit, the Parfenov horizon, has been established.
Geochemistry of radon waters in the south of Siberia
Authors: Novikov D. A. , Kopylova Yu. G. , Khvaschevskaya A. A. , Maksimova A. A. , Sukhorukova A. F. , Derkachev A. S. , Dultsev F. F. , Chernykh A. V.
Published: October 2022

The geochemical features of 22 deposits and manifestations of radon waters in the south of Siberia (some of them for the first time) are considered. The studied waters are very diverse in terms of gas and chemical composition, total mineralization, geochemical parameters of the environment, and temperature. Three groups of radon waters have been established according to the Eh value: oxidizing conditions, transitional and reducing. Due to the wide variety of waters in terms of chemical composition, some of the studied objects belong to several groups at once, for example, the Choigan natural complex, the Skalinsky, Novobibeevskoye, and Sedova Zaimka manifestations. The concentrations of natural radionuclides are (mg/dm): U (3.68∙10 - 1.40), Th (4.04∙10 - 2.16∙10). The activity of dissolved radon (Rn) varies from 4 to 2257 Bq/dm. The Th/U ratio varies from 2.63∙10 to 26, and its maximal values were detected in ultrafresh, thermal, alkaline waters with reductive geochemical medium in which thorium possesses increased migration capacity. Quite contrary, uranium migrates in the oxidative geochemical setting.
Regional forecast of the outlooks for implementation of CCUS projects at the territory of the Russian Federation
Authors: Novikov D. A. , Dultsev F. F. , Yurchik I. I. , Sadykova Ya. V. , Derkachev A. S. , Chernykh A. V. , Maksimova A. A.
Published: October 2022

The paper proposes the concept of a regional assessment of the outlooks for implementation of CCUS (carbon capture, utilization and storage) projects at the territory of the Russian Federation. Three groups of criteria are considered: general hydrogeological, tectonic and environmental. For the first time, the categories of lands with high, medium, low prospects and unpromising ones are singled out. A map of the outlooks for the implementation of CCUS projects at the territory of the Russian Federation was compiled according to the criteria of the regional level on a scale of 1: 2500,000 in the form of an ArcGis project, including its interactive analogue with access via the Internet. The performed analysis of the hydrogeological features of the territory of the Russian Federation made it possible to identify 56 structures with high, 6 with medium and 29 with low prospects at the regional stage. The most promising areas are confined to three hydrogeological regions: the East European, East Siberian platforms and the West Siberian Plate.
Geoelectrical structure of the Gusinoozerskaya basin (Western Transbaikalia) based on archival vertical electrical soundings data interpretation
Authors: Sanchaa A. M. , Neverdova N. N.
Published: October 2022

N this article we present the results of the archival VES data, obtained in the 50s of the last century on the territory of the Gusinoozerskaya depression of Western Transbaikalia, which is the reference for the “Transbaikal type” depression system. Interpretation made using a horizontally layered model, also the two-dimensional inversion performed. As a result, quantitative geoelectric characteristics of the Gusinoozerskaya depression deposits were obtained, and faults were identified. The maximum depth up to the reference horizon are in the southern part of the Gusinoozerskaya basin and reach 2300 m, as well as in the Ubukunskaya depression (up to 2200 m).
New lithological and biostratigraphic data from the Middle Ordovician of the Teletskoe Lakeside, Altai
Authors: Sennikov N. V. , Obut O. T. , Timokhin A. V.
Published: October 2022

New data on lithology, stratigraphy and paleontology of the Middle Ordovician strata cropped out in the Teletskoe Lakeside, Altai, are discussed. New section of the Samysh Formation is described. Plates with obtained collection of conodonts and trilobites are presented.
Biostratigraphic characteristic of the Lower Cretaceous NKh beds in northeastern Western Siberia
Authors: Rozbaeva G. L. , Marinov V. A. , Igolnikov A. E.
Published: October 2022

The lithostratigraphic framework of the lower part of the Cretaceous deposits in northeastern Western Siberia is considered. The paleontological characteristics of sandstone beds of the NKh group have been refined, and biostratigraphic dating of the beds has been performed.
Natural reservoir facial-petroleum field analysis for oil and gas field development
Authors: Chernova O. S.
Published: October 2022

The article describes the method of facies analysis, the relevance of which is determined by the difficulties in the study of genetic spatial heterogeneity of productive natural reservoirs by traditional, mainly "remote" methods, such as geophysical well surveys (GIS) and seismic surveys. In these conditions the facies analysis is of paramount importance: both in traditional for sedimentary strata and in its refined or new modifications. Most often, the sedimentological study uses the developments known as the facies-cyclic analysis, which predetermines a clear methodology of research, providing for a sequential separation of facies - establishment of cycles - correlation of sections - paleogeographic reconstruction, with obligatory "reverse" verification of constructions at each successive stage. However, in today's reality, the classical study of oil and gas exploration and production is practically impossible because of incomplete core sampling, fragmentation of its removal, a priori insufficient amount of core material, especially for unproductive horizons. With the mentioned non-compliance of facies-cyclic "canons" it is suggested to realize direct and direct use of facies studies, realized on the borehole cores for sedimentological and petrophysical modeling of geological natural systems. Thus, consciously removing the problems associated with the allocation of cycles, diachrony of layers of migratory nature, thus having a direct transition to the solution of practical oil and gas problems, based on a reliable facies basis. The method is developed for deep productive horizons, inaccessible to direct observation, in conditions of limited actual data (small number of cores).
Methodological aspects of determining the porosity and saturation of the pore space of the core of the Bazhenov formation
Authors: Saitov R. M. , Gorshkov A. M.
Published: October 2022

A method for determining the porosity of core samples of ultra-low-permeability rocks is presented and tested. The core samples of the Bazhenov formation were the object of research. The proposed method is based on the GRI method (Gas Research Institute), adapted for rocks of the Bazhenov formation. To calculate the coefficient of gas-saturated and open porosity, bulk and mineralogical densities were determined both for samples in a state of natural saturation and after extraction. Determination of bulk density was carried out by the gas-volumetric method and the modified method of liquid saturation. Determination of the mineralogical density was carried out by the gas volumetric method. The measurement of water saturation of the crushed core samples was carried out in the Dean-Stark apparatus. The gas saturation of core samples was calculated based on the results of determining the coefficient of gas-saturated and open porosity. Gas saturation was calculated based on the results of determining the coefficient of gas-saturated and open porosity. The oil saturation was determined by the calculation method based on the material balance.
Substantiation of reservoirs in the Bazhenov Formation in the Malobalykskaya and neighboring areas (KhMAO, Western Siberia)
Authors: Fomin M. A. , Zamirailova A. G. , Saitov R. M. , Sotnich I. S.
Published: October 2022

The article presents the results of a comprehensive interpretation of new results of laboratory studies of the core material of the Bazhenov formation and well logging data for three wells. A set of criteria for identifying reservoir intervals is substantiated, which includes the results of determining the chemical and mineral-component composition of rocks, the pyrolytic characteristics of the organic matter contained in them, as well as the petrophysical properties of these deposits. It has been established that the reservoirs can be silicites occurring in the middle part of the Bazhenov formation section - they have a high open porosity and the presence of mobile oil, confirmed by the inflow in one of the wells. It is shown that at a distance of 15-20 km this siliceous interval may not have reservoir properties, which is probably due to a different filling of the void space of these rocks. Carbonate and phosphorite rocks, which are also reservoirs in some fields in this region, do not contain mobile oil in the studied sections. It is substantiated that well logging data can only be used to identify possible productive intervals, the study of which by laboratory methods will make it possible to establish and forecast the presence or absence of reservoirs in them.
Hsa_circ_0008870 suppresses bone formation of growth plate through inhibition of miR-185-3p/ MAPK1 axis in idiopathic short stature
Authors: Wu Zhiwen , Yuan Jinghong , Li Jiantian , Du Zhi , Yin Ming , Cheng Xigao , Liu Xijuan , Jia Jingyu
Published: October 2022

Idiopathic short stature (ISS) is the most common clinical cause of the short stature with an unclear aetiology and a lack of effective treatment. Circular RNAs have been shown to play a significant regulatory role through various signal transduction pathways in a variety of diseases in recent years. However, the role of circular RNAs on ISS is not yet well-understood and requires a special attention. The differentially expressed circular RNAs were screened by microarray chip analysis, and RT-qPCR was used to verify the expression of hsa_circ_0008870 in ISS patients. Subsequently, in vitro and in vivo experiments were conducted to determine the biological functions of hsa_circ_0008870 in ISS. The authors first confirmed that hsa_ circ_0008870 was downregulated in ISS children. Meanwhile, we also observed that the downregulated hsa_circ _0008870 significantly inhibited chondrocyte proliferation and endochondral ossification in vivo and in vitro. Mechanistically, hsa_circ_0008870 regulates MAPK1 expression by sponge miR-185-3p. This mechanism of action was further verified through rescue experiments. Finally, the authors revealed that the silencing of hsa_circ_0008870 induces low expression of MAPK1 by impairing the sponge action of miR-185-3p, thereby inhibiting chondrocyte proliferation, hypertrophy, and endochondral ossification, which results in a short stature phenotype. In addition to these, we also observed an interesting phenomenon that upregulated of miR-185-3p can in turn inhibit the expression of hsa_circ_0008870 in chondrocytes. This suggests that hsa_circ_0008870 could potentially serve as a therapeutic target for the treatment of ISS.
Modified hollow mesoporous silica nanoparticles as immune adjuvant-nanocarriers for photodynamically enhanced cancer immunotherapy
Authors: Li Qianru , Liu Qianqian , Li Heli , Dong Liyun , Zhou Yajie , Zhu Jintao , Yang Liu , Tao Juan
Published: October 2022

Nanomedicine has demonstrated great potential in enhancing cancer immunotherapy. However, nanoparticle (NP)-based immunotherapy still has limitations in inducing effective antitumor responses and inhibiting tumor metastasis. Herein, polyethylenimine (PEI) hybrid thin shell hollow mesoporous silica NPs (THMSNs) were applied as adjuvant-nanocarriers and encapsulated with very small dose of photosensitizer chlorine e6 (Ce6) to realize the synergy of photodynamic therapy (PDT)/immunotherapy. Through PEI etching, the obtained Ce6@THMSNs exhibited enhanced cellular internalization and endosome/lysosome escape, which further improved the PDT efficacy of Ce6@THMSNs in destroying tumor cells. After PDT treatment, the released tumor-associated antigens with the help of THMSNs as adjuvants promoted dendritic cells maturation, which further boosted CD8+ cytotoxic T lymphocytes activation and triggered antitumor immune responses. The in vivo experiments demonstrated the significant potency of Ce6@THMSNs-based PDT in obliterating primary tumors and inducing persistent tumor-specific immune responses, thus preventing distant metastasis. Therefore, we offer a THMSNs-mediated and PDT-triggered nanotherapeutic system with immunogenic property, which can elicit robust antitumor immunity and is promising for future clinical development of immunotherapy.
Region-dependent mechanical characterization of porcine thoracic aorta with a one-to-many correspondence method to create virtual datasets using uniaxial tensile tests
Authors: Ryu Dongman , Baek Seungik , Kim Jungsil
Published: October 2022

The simulation of the cardiovascular system and in silico clinical trials have garnered attention in the biomedical engineering field. Physics-based modeling is essential to associate with physical and clinical features. In physics-based constitutive modeling, the identification of the parameters and estimation of their ranges based on appropriate experiments are required. Uniaxial tests are commonly used in the field of vascular mechanics, but they have limitations in fully characterizing the regional mechanical behavior of the aorta. Therefore, this study is aimed at identifying a method to integrate constitutive models with experimental data to elucidate regional aortic behavior. To create a virtual two-dimensional dataset, a pair of uniaxial experimental datasets in the longitudinal and circumferential directions was combined using a one-to-many correspondence method such as bootstrap aggregation. The proposed approach is subsequently applied to three constitutive models, i.e., the Fung model, Holzapfel model, and constrained mixture model, to estimate the material parameters based on the four test regions of the porcine thoracic aorta. Finally, the regional difference in the mechanical behavior of the aorta, the correlation between the experimental characteristics and model parameters, and the inter-correlation of the material parameters are confirmed. This integrative approach will enhance the prediction capability of the model with respect to the regions of the aorta.
Potential of gut microbiota for lipopolysaccharide biosynthesis in European women with type 2 diabetes based on metagenome
Authors: Dong Ying , Wang Pan , Yang Xinchuan , Chen Mulei , Li Jing
Published: October 2022

The abnormal accumulation of lipopolysaccharide (LPS) plays a crucial role in promoting type 2 diabetes (T2D). However, the capability of the gut microbiota to produce LPS in patients with T2D is still unclear, and evidence characterizing the patterns of gut microbiota with LPS productivity remains rare. This study aimed to uncover the profiles of LPS-biosynthesis-related enzymes and pathways, and explore the potential of LPS-producing gut microbiota in T2D. The gut metagenomic sequencing data from a European female cohort with normal glucose tolerance or untreated T2D were analyzed in this study. The sequence search revealed that the relative abundance of the critical enzymes responsible for LPS biosynthesis was significantly high in patients with T2D, especially for N-acetylglucosamine deacetylase, 3-deoxy-D-manno-octulosonic-acid transferase, and lauroyl-Kdo2-lipid IVA myristoyltransferase. The functional analysis indicated that a majority of pathways involved in LPS biosynthesis were augmented in patients with T2D. A total of 1,173 species from 335 genera containing the gene sequences of LPS enzymes, including LpxA/B/C/D/H/K/L/M and/or WaaA, coexisted in controls and patients with T2D. Critical taxonomies with discriminative fecal abundance between groups were revealed, which exhibited different associations with enzymes. Moreover, the identified gut microbial markers had correlations with LPS enzymes and were subsequently associated with microbial pathways. The present findings delineated the potential capability of gut microbiota toward LPS biosynthesis in European women and highlighted a gut microbiota−based mechanistic link between the disturbance in LPS biosynthesis and T2D. The restoration of LPS levels through gut microbiota manipulation might offer potential approaches for preventing and treating T2D.
Boosting CO2 hydrogenation to methane over Ni-based ETS-10 zeolite catalyst
Authors: Xiang Mei , Gao Zhangxi , Ji Xiaonan , Li Dantong , Deng Yaoyao , Ding Yalong , Yu Chi , Zhang Wei , Zhang Zhenwei , Wu Zeying , Zhou Jiancheng
Published: October 2022

The activation and conversion of the CO2 molecule have always been the most vexing challenge due to its chemical inertness. Developing highly active catalysts, which could overcome dynamic limitations, has emerged as a provable and effective method to promote CO2 activation–conversion. Herein, ETS-10 zeolite–based catalysts, with active nickel species introduced by in situ doping and impregnation, have been employed for CO2 methanation. Conspicuous CO2 conversion (39.7%) and perfect CH4 selectivity (100%) were achieved over the Ni-doped ETS-10 zeolite catalyst at 280°C. Comprehensive analysis, which include X-ray diffraction, N2 adsorption–desorption, SEM, TEM, H2 chemisorption, CO2 temperature programmed desorption, and X-ray photoelectron spectroscopy, was performed. Also, the results indicated that the resultant hierarchical structure, high metal dispersion, and excellent CO2 adsorption–activation capacity of the Ni-doped ETS-10 zeolite catalyst played a dominant role in promoting CO2 conversion and product selectivity.
Influence of Aikido and Taijiquan-Tuishou on Contact Improvisation
Authors: Gómez-Lozano Sebastián , Vargas-Macías Alfonso , Kelly-Lahon Clare , León Kiko , García-Sottile María Eugenia
Published: October 2022

Oriental Martial Arts such as Taijiquan-Tuishou and Aikido represent some of the Asian influences on western culture resulting from immigration from China and Japan to the United States of America during the middle of the 20th century. Contact Improvisation, a style of post-modern dance performed in pairs, is one of the manifestations enriched by this oriental influence. The purpose of this manuscript is to document which dynamic, proprioceptive and somatic elements were transferred to the choreographic language of Contact Improvisation from these martial arts. In the case of Contact Improvisation, the most important technical components highlighted include: center of gravity, weight sharing, point of contact, sphericity, rolling, the embryonic relationship of axial axis and limbs, ki and proprioceptive communication. Although an evolution in the interpretation of the meanings and uses of this particular dance form may exist, we can nonetheless establish some kinesthetic communication codes and strategies derived directly from its original sources. These sources belong to martial arts such as Aikido and Tuishou or Taijiquan and are essential to the intercultural communication component of the Contact Improvisation duo since they involve the learning of fundamentals and principles of non-verbal interaction considered as specific for the mastery of intercorporeality. This is discussed in light of the work of Mark Young, a Contact Improvisation choreographer who maintains Paxton's legacy of roll technique documented in “Material for the Spine” by perfecting the execution and technical variations of helix rolls in a constructed system of spirals. This concurrence of strategies adopted from Aikido and Taijiquan and the usefulness of these elements in terms of performing this partner dance would appear to be key in the understanding of Contact Improvisation.
The potential of heparin-induced extracorporeal LDL/fibrinogen precipitation (H.E.L.P.)-apheresis for patients with severe acute or chronic COVID-19
Authors: Jaeger Beate Roxane , Arron Hayley Emma , Kalka-Moll Wiltrud M. , Seidel Dietrich
Published: October 2022

Patients with long COVID and acute COVID should benefit from treatment with H.E.L.P. apheresis, which is in clinical use for 37 years. COVID-19 can cause a severe acute multi-organ illness and, subsequently, in many patients the chronic illness long-COVID/PASC. The alveolar tissue and adjacent capillaries show inflammatory and procoagulatory activation with cell necrosis, thrombi, and massive fibrinoid deposits, namely, unsolvable microthrombi, which results in an obstructed gas exchange. Heparin-induced extracorporeal LDL/fibrinogen precipitation (H.E.L.P.) apheresis solves these problems by helping the entire macro- and microcirculation extracorporeally. It uses unfractionated heparin, which binds the spike protein and thereby should remove the virus (debris). It dissolves the forming microthrombi without bleeding risk. It removes large amounts of fibrinogen (coagulation protein), which immediately improves the oxygen supply in the capillaries. In addition, it removes the precursors of both the procoagulatory and the fibrinolytic cascade, thus de-escalating the entire hemostaseological system. It increases myocardial, cerebral, and pulmonary blood flow rates, and coronary flow reserve, facilitating oxygen exchange in the capillaries, without bleeding risks. Another factor in COVID is the “cytokine storm” harming microcirculation in the lungs and other organs. Intervention by H.E.L.P. apheresis could prevent uncontrollable coagulation and inflammatory activity by removing cytokines such as interleukin (IL)-6, IL-8, and TNF-α, and reduces C-reactive protein, and eliminating endo- and ecto-toxins, without touching protective IgM/IgG antibodies, leukocyte, or platelet function. The therapy can be used safely in combination with antiviral drugs, antibiotics, anticoagulants, or antihypertensive drugs. Long-term clinical experience with H.E.L.P. apheresis shows it cannot inflict harm upon patients with COVID-19.
Case report: Characterization of a rare pathogenic variant associated with loss of COL3A1 expression in vascular Ehlers Danlos syndrome
Authors: Manhas Janvie , Lohani Lov Raj , Seethy Ashikh , Kumar Uma , Gamanagatti Shivanand , Sen Sudip
Published: October 2022

The vascular subtype of Ehlers Danlos Syndrome (vEDS) is a rare connective tissue disorder characterized by spontaneous arterial, bowel or organ rupture. The diagnosis of vEDS is established in a proband by identification of a heterozygous pathogenic variant in the alpha-1 gene of type III collagen (COL3A1) by molecular analysis. In this report, we present a case of vEDS with life threatening, spontaneous arterial dissections in association with an uncharacterized rare variant of COL3A1, exon19:c.1340G > A. Primary culture of patient skin fibroblasts followed by immunofluorescence revealed a complete absence of COL3A1 protein expression as well as altered morphology. Electron microscopy of the cultured fibroblasts showed abnormal vacuoles in the cytoplasm suggestive of a secretory defect. In this study, we have performed functional characterization of the COL3A1 exon19:c.1340G > A variant for the first time and this may now be classified as likely pathogenic in vEDS.
Identification of energy metabolism-related biomarkers for risk prediction of heart failure patients using random forest algorithm
Authors: Chen Hao , Jiang Rui , Huang Wentao , Chen Kequan , Zeng Ruijie , Wu Huihuan , Yang Qi , Guo Kehang , Li Jingwei , Wei Rui , Liao Songyan , Tse Hung-Fat , Sha Weihong , Zhuo Zewei
Published: October 2022

Objective

Energy metabolism plays a crucial role in the improvement of heart dysfunction as well as the development of heart failure (HF). The current study is designed to identify energy metabolism-related diagnostic biomarkers for predicting the risk of HF due to myocardial infarction.

Methods

Transcriptome sequencing data of HF patients and non-heart failure (NF) people (GSE66360 and GSE59867) were obtained from gene expression omnibus (GEO) database. Energy metabolism-related differentially expressed genes (DEGs) were screened between HF and NF samples. The subtyping consistency analysis was performed to enable the samples to be grouped. The immune infiltration level among subtypes was assessed by single sample gene set enrichment analysis (ssGSEA). Random forest algorithm (RF) and support vector machine (SVM) were applied to identify diagnostic biomarkers, and the receiver operating characteristic curves (ROC) was plotted to validate the accuracy. Predictive nomogram was constructed and validated based on the result of the RF. Drug screening and gene-miRNA network were analyzed to predict the energy metabolism-related drugs and potential molecular mechanism.

Results

A total of 22 energy metabolism-related DEGs were identified between HF and NF patients. The clustering analysis showed that HF patients could be classified into two subtypes based on the energy metabolism-related genes, and functional analyses demonstrated that the identified DEGs among two clusters were mainly involved in immune response regulating signaling pathway and lipid and atherosclerosis. ssGSEA analysis revealed that there were significant differences in the infiltration levels of immune cells between two subtypes of HF patients. Random-forest and support vector machine algorithm eventually identified ten diagnostic markers (MEF2D, RXRA, PPARA, FOXO1, PPARD, PPP3CB, MAPK14, CREB1, MEF2A, PRMT1) for risk prediction of HF patients, and the proposed nomogram resulted in good predictive performance (GSE66360, AUC = 0.91; GSE59867, AUC = 0.84) and the clinical usefulness in HF patients. More importantly, 10 drugs and 15 miRNA were predicted as drug target and hub miRNA that associated with energy metabolism-related genes, providing further information on clinical HF treatment.

Conclusion

This study identified ten energy metabolism-related diagnostic markers using random forest algorithm, which may help optimize risk stratification and clinical treatment in HF patients.
Risk of severe COVID-19 infection in persons with diabetes during the first and second waves in Denmark: A nationwide cohort study
Authors: Stidsen Jacob V. , Green Anders , Rosengaard Louise , Højlund Kurt
Published: October 2022

Objective

Coronavirus disease-2019 (COVID-19) increases risk of hospitalization and death in diabetes and diabetes-related conditions. We examined the temporal trends in COVID-19-related hospitalization and mortality in the total Danish population by diabetes and diabetes-related conditions in the two first waves of COVID-19 in Denmark.

Materials and methods

We identified all persons with diabetes in the whole Danish population using national registries. COVID-19-related risks of hospitalization and death were assessed using Cox regression analysis in wave 1 (1 March-31 August 2020) and wave 2 (1 September 2020-28 February 2021) of the pandemic for persons with (n=321,933) and without diabetes (n=5,479,755). Analyses were stratified according to status of hypertension, obesity, cardiovascular and microvascular disease.

Results

The cumulative incidence of COVID-19 hospitalization increased from wave 1 to wave 2 in both persons without (from 4 to 10 in 10,000) and with diabetes (from 16 to 54 per 10,000). The relative risk of hospitalization, however, increased more in patients with diabetes compared to persons without (age-, sex- and co-morbidity-adjusted HR [aHR] 1.40 (95% CI 1.27, 1.55) versus 1.76 (1.65, 1.87), p<0.001 for interaction with wave). The mortality rate, according to the whole population, increased similarly in persons without and with diabetes from wave 1 to wave 2 (from 0.63 to 1.5 versus from 4.3 to 10 in 10,000; aHR 1.65; 1.34, 2.03 and 1.64; 1.43, 1.88). However, when mortality was restricted to the hospitalized population, the crude mortality fell from 26.8% to 19.6% in persons with diabetes, while only a minor decrease was seen in persons without diabetes (from 16.7% to 15.5%).

Conclusion

The risk of COVID-19-related hospitalization increased more in persons with than without diabetes from wave 1 to wave 2 of the COVID-19 pandemic in the Danish population. However, mortality according to the whole population did not change, due to reduced mortality among hospitalized persons with diabetes.
Body mass index, basal insulin and glycemic control in children with type 1 diabetes treated with the advanced hybrid closed loop system remain stable - 1-year prospective, observational, two-center study
Authors: Seget Sebastian , Jarosz-Chobot Przemysława , Ochab Agnieszka , Polanska Joanna , Rusak Ewa , Witoszek Paulina , Chobot Agata
Published: October 2022

Background

Information on the influence of insulin treatment using advanced hybrid closed loop systems (AHCL) on body weight of young patients with type 1 diabetes (T1D) is scarce. The aim of this study was to observe whether there were any changes in body mass index (BMI) of children and adolescents with T1D treated using the Medtronic Minimed 780G AHCL after 1 year of follow up and to analyze potential associations between these changes and the insulin doses.

Materials and methods

For 50 children and adolescents (age 5.4-16.8 years, 24 (48%) boys, T1D for 3.9 ± 2.56 years) using an AHCL system anthropometric and AHCL data were collected prospectively. BMI Z-scores and two-week AHCL records obtained after AHCL enrollment were compared with data after 6 months and also 1 year after starting AHCL.

Results

The BMI Z-score of the patients at 1 year follow-up did not change from time of AHCL initiation (0.51 ± 2.79 vs 0.57 ± 2.85, p>0.05). There was a slight increase in total daily insulin per kg of body weight (0.67 ± 0.21 U/kg vs 0.80 ± 0.21 U/kg, p <0.001), but the percent of basal insulin was unchanged (34.88 ± 6.91% vs 35.08 ± 6.30%, p>0.05). We observed also no change (AHCL start vs after 1 year) in glycemic control parameters: average sensor glucose (131.36± 11.04 mg/dL vs 132.45 ± 13.42 mg/dL, p>0.05), coefficient of variation (34.99± 5.17% vs 34.06 ± 5.38%, p>0.05), glucose management indicator (6.45 ± 0.26% vs 6.48 ± 0.32%, p>0.05), and time spent in the range of 70–180 mg/dL (79.28 ± 8.12% vs 80.40 ± 8.25%, p>0.05).

Conclusion

During the 1 year of follow-up the BMI of children and adolescents with T1D treated with an AHCL system remained stable. Although there was a slight increase in the total daily insulin dose, the percent of basal insulin was unchanged. The patients maintained recommended glycemic control.
Advanced glycation end-products are associated with diabetic neuropathy in young adults with type 1 diabetes
Authors: Al-Saoudi Elaf , Christensen Marie M. B. , Nawroth Peter , Fleming Thomas , Hommel Eva E. , Jørgensen Marit E. , Fleischer Jesper , Hansen Christian S.
Published: October 2022

Aims/hypothesis

Advanced glycation end-products (AGEs) may contribute to the development of diabetic neuropathy. In young adults with type 1 diabetes, we aimed to investigate the association between AGEs and cardiovascular autonomic neuropathy (CAN) and distal symmetric polyneuropathy (DSPN).

Methods

This cross-sectional study comprised 151 young adults. CAN was assessed by cardiovascular autonomic reflex tests; lying-to-standing test, deep breathing test (E/I), Valsalva manoeuvre, and heart rate variability indices; and the mean square of the sum of the squares of differences between consecutive R-R intervals and standard deviation of normal-to-normal intervals (SDNN), high- (HF) and low-frequency (LF) power, total frequency power, and the LF/HF ratio. DSPN was assessed by light touch, pain and vibration perception threshold (VPT), neuropathy questionnaires, and objective measures. AGEs were analysed in four groups using z-scores adjusted for relevant confounders and multiple testing: i) “glycolytic dysfunction”, ii) “lipid peroxidation”, iii) “oxidative stress”, and iv) “glucotoxicity”.

Results

A higher z-score of “glycolytic dysfunction” was associated with higher VPT (4.14% (95% CI 1.31; 7.04), p = 0.004) and E/I (0.03% (95% CI 0.01; 0.05), p = 0.005), “lipid peroxidation” was associated with higher LF/HF ratio (37.72% (95% CI 1.12; 87.57), p = 0.044), and “glucotoxicity” was associated with lower SDNN (−4.20% (95% CI −8.1416; −0.0896), p = 0.047). No significance remained after adjustment for multiple testing.

Conclusions/interpretations

In young adults with type 1 diabetes, increased levels of AGEs involving different metabolic pathways were associated with several measures of CAN and DSPN, suggesting that AGEs may play a diverse role in the pathogeneses of diabetic neuropathy.
The relationship between thyroid disorders and vitamin A.: A narrative minireview
Authors: Capriello S. , Stramazzo I. , Bagaglini M. F. , Brusca N. , Virili C. , Centanni M.
Published: October 2022

The terms “vitamin A” and “retinoids” encompass a group of fat-soluble compounds essential for human nutrition. Some of them (retinol, retinal, 9-cis-retinoic acid, tretinoin, and 13-cis-retinoic acid) are fully natural, while others are synthetic compounds used mostly for therapeutic purposes. Some evidence indicates that the nutritional status of these retinoids (i.e., the presence or absence of deficiency) is able to modulate thyroid gland metabolism. Vitamin A deficiency is tightly correlated with structural and functional impairment of the thyroid gland and is often associated with iodine deficiency. Furthermore, retinoids are involved in different immune functions, as well as in the process of activation, proliferation, and differentiation of regulatory T cells (Treg). This is particularly significant given the high prevalence of thyroid autoimmune disorders, whose pathogenesis seems to be related to the altered homeostasis of regulatory T cells. Retinoids are also involved in the modulation of gene expression via their interaction with nuclear receptors, and they also act as cofactors in cell growth and differentiation. The ability of retinoic acid to increase iodine uptake and sodium-iodine symporter activity in human thyroid cancer cell lines suggests that some retinoids and their derivatives may be of use in the treatment of different thyroid tumors. This minireview summarizes the current knowledge on the link between nutritional intake of vitamin A and various thyroid disorders.
Novel pituitary actions of GnRH in teleost: The link between reproduction and feeding regulation
Authors: Li Wei , Du Ruixin , Xia Chuanhui , Zhang Huiying , Xie Yunyi , Gao Xiaowen , Ouyang Yu , Yin Zhan , Hu Guangfu
Published: October 2022

Gonadotropin-releasing hormone (GnRH), as a vital hypothalamic neuropeptide, was a key regulator for pituitary luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in the vertebrate. However, little is known about the other pituitary actions of GnRH in teleost. In the present study, two GnRH variants (namely, GnRH2 and GnRH3) and four GnRH receptors (namely, GnRHR1, GnRHR2, GnRHR3, and GnRHR4) had been isolated from grass carp. Tissue distribution displayed that GnRHR4 was more highly detected in the pituitary than the other three GnRHRs. Interestingly, ligand–receptor selectivity showed that GnRHR4 displayed a similar and high binding affinity for grass carp GnRH2 and GnRH3. Using primary culture grass carp pituitary cells as model, we found that both GnRH2 and GnRH3 could not only significantly induce pituitary reproductive hormone gene (GtHα, LHβ, FSHβ, INHBa, secretogranin-2) mRNA expression mediated by AC/PKA, PLC/IP3/PKC, and Ca2+/CaM/CaMK-II pathways but also reduce dopamine receptor 2 (DRD2) mRNA expression via the Ca2+/CaM/CaMK-II pathway. Interestingly, GnRH2 and GnRH3 could also stimulate anorexigenic peptide (POMCb, CART2, UTS1, NMBa, and NMBb) mRNA expression via AC/PKA, PLC/IP3/PKC, and Ca2+/CaM/CaMK-II pathways in grass carp pituitary cells. In addition, food intake could significantly induce brain GnRH2 mRNA expression. These results indicated that GnRH should be the coupling factor to integrate the feeding metabolism and reproduction in teleost.