bluuebunny/crossref_metadata_2025 huggingface dataset:
Poetics of reduplicative word formation: evidence from a rating and recall experiment
Authors: Kentner Gerrit , Franz Isabelle , Menninghaus Winfried
Published: May 2022

Reduplicative words like chiffchaff or helter-skelter are part of ordinary language use yet most often found in substandard registers in which attitudinal and expressive meaning components are iconically foregrounded. In a rating experiment using nonwords that either conform to, or deviate from, conventional reduplicative patterns in German, the present study identified affective meaning dimensions, judgments of familiarity and esthetic evaluations of sound qualities associated with such words. In a subsequent recall test, we examined the respective mnemonic potential of the different types of reduplication. Results suggest that, in the absence of semantic content, reduplicative forms are inherently associated with several affective meaning associations that are generally considered positive. Two types of reduplicative patterns, namely full reduplication and [i-a]-vowel-alternating reduplication, boost these positive effects to a particularly pronounced degree, leading to an increase in perceived euphony, funniness, familiarity, appreciation, and positive belittling (cuteness) and, at the same time, a decrease in arousal. These two types also turn out to be particularly memorable when compared both to other types of reduplication and to non-reduplicative structures. This study demonstrates that reduplicative morphology may in and of itself, that is, irrespective of the phonemic and the semantic content, contribute to the affective meaning and esthetic evaluation of words.
Regional Ambassadors or State Agents? Assessing the Role of Catalan Cabinet Ministers in Spain
Authors: Fernández Rivera Cristina , Harguindéguy Jean-Baptiste , Rodríguez Teruel Juan
Published: May 2022

This study focuses on Catalan cabinet ministers in democratic Spain with a view to understanding what function they perform in the central government: regional ambassador or state agent? To this end, this analysis draws on a sub-dataset comprised of 22 Catalan cabinet ministers taken from a general pool of 220 cabinet ministers and 371 ministerial appointments from 1977 to 2021. Our findings demonstrate, first, that no Catalan cabinet minister has ever reached the position of Prime Minister and Catalans constitute a kind of ministerial “middle class” occupying intermediate positions in the cabinet. Second, the examination of career paths and publications of Catalan ministers shows that their role varied according to circumstances. Third, in this article we argue that those variations can be best interpreted as a delegation between principal and agent relying on two main variables, namely the type of party they belong to in Catalonia and the parliamentarian majority sustaining the party controlling the Spanish cabinet.
Defining the Borderlands: Sino-Soviet Border Talks and the Nationalities Issue (1987–1991)
Authors: Tagirova Alsu
Published: May 2022

In the 1980s, the Soviet Union and China resurveyed their border in order to restart their long-stalemated border negotiations. These negotiations resulted in only a partial border settlement: the agreement was signed in 1991. By the end of 1989, nationalities openly expressing their wish to secede from the Soviet Union caused the Soviet government to slow down the negotiation process, and Moscow insisted on setting aside the most contentious sections. China’s nationalities issue had the opposite effect on Zhongnanhai: Chinese leaders wished to settle the entire Sino-Soviet border as quickly as possible. However, once the collapse of the Soviet Union became imminent, the Chinese saw advantages of delaying the negotiations on the disputed sections of the border. They calculated that would allow for China to negotiate with weaker, newly independent countries.
A NEW -ANALOGUE OF VAN HAMME’S (A.2) SUPERCONGRUENCE
Authors: GUO VICTOR J. W.
Published: May 2022

We give a new q-analogue of the (A.2) supercongruence of Van Hamme. Our proof employs Andrews’ multiseries generalisation of Watson’s
$_{8}\phi _{7}$
transformation, Andrews’ terminating q-analogue of Watson’s
$_{3}F_{2}$
summation, a q-Watson-type summation due to Wei–Gong–Li and the creative microscoping method, developed by the author and Zudilin [‘A q-microscope for supercongruences’, Adv. Math.346 (2019), 329–358]. As a conclusion, we confirm a weaker form of Conjecture 4.5 by the author [‘Some generalizations of a supercongruence of van Hamme’, Integral Transforms Spec. Funct.28 (2017), 888–899].
Vitamin E protective effects on genomic and cellular damage caused by paediatric preventive supplementation for anaemia: an experimental model
Authors: Gambaro Rocío Celeste , Seoane Analía , Padula Gisel
Published: May 2022

Iron deficiency is the leading cause of anaemia. In Argentina, the prevalence of anaemia and iron deficiency is very high; for that reason, the Argentine Society of Pediatrics recommends daily ferrous sulphate supplementation as a preventive treatment strategy. Alternatively, weekly ferrous sulphate supplementation has also been shown to be effective for anaemia prevention. Excess iron could be related to oxidative stress, which may in turn cause cytomolecular damage. Both can be prevented with vitamin E supplementation. We evaluated the effect of both daily and weekly ferrous sulphate supplementation combined with two doses of vitamin E on cell viability, oxidative stress and cytomolecular damage in peripheral blood cultured in vitro. The experimental design included the following groups: untreated negative control, two vitamin E controls (8·3 and 16·6 µg/ml), weekly ferrous sulphate supplementation (0·55 mg/ml) with each vitamin E dose, daily ferrous sulphate supplementation (0·14 mg/ml) with each vitamin E dose and a positive control. Daily ferrous sulphate supplementation decreased cell viability and increased the levels of reactive oxygen species, lipid peroxidation and cytomolecular damage (P < 0·5) compared with the weekly supplementation, probably due to the excess iron observed in the former. Vitamin E seemed to reduce ferrous sulphate-induced oxidative stress and genomic damage.
Comparative reproductive biology of pre-, imaginal, and neotenic castes of the Asian termiteCoptotermes gestroi(Blattaria, Isoptera, Rhinotermitidae)
Authors: Costa-Leonardo Ana Maria , Janei Vanelize , da Silva Iago Bueno
Published: May 2022

The Asian subterranean termiteCoptotermes gestroiis a worldwide structural pest, although its reproductive biology has been poorly investigated due to a cryptic habit and occurrence of polycalic nests. In this study, we investigated ovarian development and oogenesis in different-aged females ofC. gestroi: fourth-instar nymphs, non-functional neotenics, alates, and functional queens. We show that the ovaries develop gradually according to their age and functionality, as younger individuals possess immature oocytes, whereas alates and functional queens always undergo vitellogenesis. Oocytes were classified into previtellogenic (stages I, II, and III) or vitellogenic (stages IV, V, and VI). Ovary development varied among non-functional neotenics, and a rapid differentiation and/or the presence of primary reproductives are believed to influence such a maturation. Immature oocyte stages were shared between fourth-instar nymphs and neotenics. These characteristics, together with other neotenic features (wing buds, body pigmentation, and eye color), should be evaluated in detail aiming to clarify which nymphal instars differentiate into secondary reproductives. Oogenesis was not uniform among alate females, and cross-sectional area of terminal oocytes was significantly smaller in alates when compared to functional queens, suggesting different degrees of maturation in swarming individuals. Functional queens always had mature terminal oocytes (stage VI). Ovariole number and oocyte maturation inC. gestroirelies on several factors and may therefore differ among individuals of the same caste. Future studies should take into account these reproductive features to evaluate how they impact colony development.
Radical-right populism in Spain and the strategy of chronopolitics
Authors: Divita David
Published: May 2022

Given ongoing debates in Spain over how to reckon with its recent past, time operates as a potent site for doing politics in the Peninsula. In this article, I develop the concept of chronopolitics—that is, the discursive configuration of time or history to advance political projects in the present—by analyzing a speech from the leader of Vox, a radical-right populist party in Spain. Through detailed analysis of the text, I reveal a range of chronopolitical strategies, including blatant acts of historical revisionism and the resurrection of slogans associated with Spain's authoritarian past. I also shed insight on more subtle forms of chronopolitical action: the confusion of temporal modes, the subversion of linear perceptions of time, and metapragmatic talk about historical interpretation itself. My aim is to illuminate Vox's particular tactics of persuasion, while drawing lessons from the case of Spain about the mechanics of populist discourse in general. (Spain, Vox, populism, chronopolitics, time, history)*
Position control of a wheel-based miniature magnetic robot using neuro-fuzzy network
Authors: Salehi Mobin , Nejat Pishkenari Hossein , Zohoor Hassan
Published: May 2022

Untethered small-scale robots can accomplish tasks which are not feasible by conventional macro robots. In the current research, we have designed and fabricated a miniature magnetic robot actuated by an external magnetic field. The proposed robot has two coaxial wheels and one magnetic dipole which is capable of rolling and moving on the surface by variation in the direction of magnetic field. To generate the desired magnetic field, a Helmholtz electromagnetic coil is manufactured. To steer the robot to the desired position, at first the robot dynamics is investigated, and subsequently a controller based on a neuro-fuzzy network has been designed. Finally, the proposed controller is implemented experimentally and the performance of the control system is demonstrated.
On approximation of the analytic fixed finite time large t probability distributions in an extreme renewal process with no-mean inter-renewals
Authors: Brill Percy H. , Huang Mei Ling
Published: May 2022

We consider an extreme renewal process with no-mean heavy-tailed Pareto(II) inter-renewals and shape parameter $\alpha$ where $0\lt\alpha \leq 1$. Two steps are required to derive integral expressions for the analytic probability density functions (pdfs) of the fixed finite time $t$ excess, age, and total life, and require extensive computations. Step 1 creates and solves a Volterra integral equation of the second kind for the limiting pdf of a basic underlying regenerative process defined in the text, which is used for all three fixed finite time $t$ pdfs. Step 2 builds the aforementioned integral expressions based on the limiting pdf in the basic underlying regenerative process. The limiting pdfs of the fixed finite time $t$ pdfs as $t\rightarrow \infty$ do not exist. To reasonably observe the large $t$ pdfs in the extreme renewal process, we approximate them using the limiting pdfs having simple well-known formulas, in a companion renewal process where inter-renewals are right-truncated Pareto(II) variates with finite mean; this does not involve any computations. The distance between the approximating limiting pdfs and the analytic fixed finite time large $t$ pdfs is given by an $L_{1}$ metric taking values in $(0,1)$, where “near $0$” means “close” and “near $1$” means “far”.
Nonlinear and unbalanced urn models with two types of strategies: a stochastic approximation point of view
Authors: Idriss Soumaya
Published: May 2022

In this paper, we treat a nonlinear and unbalanced $2$-color urn scheme, subjected to two different nonlinear drawing rules, depending on the color withdrawn. We prove a central limit theorem as well as a law of large numbers for the urn composition. We also give an estimate of the mean and variance of both types of balls.
Can Non-Physician Providers Use Ultrasound to Aid in Establishing Peripheral IV Access in Patients Who are Difficult to Cannulate? A Scoping Review
Authors: Burton Samuel O. , Donovan Jake K. , Jones Samuel L. , Meadley Benjamin N.
Published: May 2022

Introduction:

Non-physician performed point-of-care ultrasound (POCUS) is emerging as a diagnostic adjunct with the potential to enhance current practice. The scope of POCUS utility is broad and well-established in-hospital, yet limited research has occurred in the out-of-hospital environment. Many physician-based studies expound the value of POCUS in the acute setting as a therapeutic and diagnostic tool. This study utilized a scoping review methodology to map the literature pertaining to non-physician use of POCUS to improve success of peripheral intravenous access (PIVA), especially in patients predicted to be difficult to cannulate.

Methods:

Ovid MEDLINE, CINAHL Plus, EMBASE, and PubMed were searched from January 1, 1990 through April 15, 2021. A thorough search of the grey literature and reference lists of relevant articles was also performed to identify additional studies. Articles were included if they examined non-physician utilization of ultrasound-guided PIVA (USGPIVA) for patients anticipated to be difficult to cannulate.

Results:

A total of 158 articles were identified. A total of 16 articles met the inclusion criteria. The majority of participants had varied experience with ultrasound, making accurate comparison difficult. Training and education were non-standardized, as was the approach to determining difficult intravenous access (DIVA). Despite this, the majority of the studies demonstrated high first attempt and overall success rates for PIVA performed by non-physicians.

Conclusion:

Non-physician USGPIVA appears to be a superior method for PIVA when difficulty is anticipated. Additional benefits include reduced requirement for central venous catheter (CVC) or intraosseous (IO) needle placement. Paramedics, nurses, and emergency department (ED) technicians are able to achieve competence in this skill with relatively little training. Further research is required to explore the utility of this practice in the out-of-hospital environment.
What about the therapist? – a quantitative exploration of the pathways from a therapist’s professional life to their personal well-being
Authors: Altaf Sidrah
Published: May 2022

Occupational stress has been identified as one of the biggest contributors to therapists’ poor mental well-being; however, these ‘wear and tear’ effects are not universal. This study aimed to distinguish between these experiences, by exploring potential avenues through which a therapist’s professional life may impact their well-being. Previous research has highlighted four avenues: self-doubt, developmental depletion, developmental growth, and years of experience. A sample of 65 cognitive behavioural therapists were recruited from four Improving Access to Psychological Therapies (IAPT) services and a cognitive behavioural therapy training course at Buckinghamshire New University. A quantitative cross-sectional survey design was employed. Each participant completed a questionnaire consisting of subscales of the ‘Development of Psychotherapist Common Core Questionnaire’ to measure potential avenues (Orlinskyet al., 1999a), and the Warwick-Edinburgh Mental Well-being Scale to measure mental well-being (Tennantet al., 2007). A multiple regression andpost-hocmediation analyses were conducted. The multiple regression analysis found three significant direct pathways predicting well-being: self-doubt, developmental depletion, and developmental growth. Thepost-hocmediation analyses found two significant indirect pathways between self-doubt and well-being mediated by developmental experience (growth and depletion). Years of experience had a non-significant direct and indirect pathway predicting well-being. The results suggested that therapists experiencing more growth, less depletion and less self-doubt experienced more positive mental well-being than those experiencing depletion and self-doubt, and less growth. When therapists experience high self-doubt, whether they also experience depletion or growth, they experience poor mental well-being. The implications for practice and future avenues of research are discussed.

Key learning aims

(1)

To understand the potential impact of therapists’ professional lives on their well-being.

(2)

To understand the role of developmental experience and self-doubt.
Exposure to p,p´-DDE during early pregnancy, anthropometry, and gestational age at birth, in a flower-growing region of Mexico
Authors: Torres-Sánchez Luisa , Escamilla-Nuñez Maria Consuelo , Cebrian Mariano E. , Ancira-Moreno Monica , Rivera-Pasquel Marta , Lope Virginia , Ramos-Treviño Juan , Blanco-Muñoz Julia
Published: May 2022

Prenatal exposure to dichlorodiphenyldichloroethylene (p,p´-DDE) may interfere with fetal development; however, studies evaluating anthropometry and gestational age at birth show inconsistent results. Typically, p,p´-DDE exposure has been measured during the third trimester and missed the key early pregnancy period. We evaluated the association between p,p´-DDE exposure before week 18 of pregnancy and anthropometry at birth, as well as gestational length, in 170 mother–child pairs from a cohort study in a flower-growing mexican region. Maternal serum p,p´-DDE concentrations were determined by gas chromatography. The associations between p,p´-DDE and z-scores of birth weight, birth length, and gestational age were evaluated by linear multiple regression models. Logistic regression models were used for low birth weight and small size for gestational age. Effect modification by child’s sex was explored. The average gestational age at the blood sample extraction was 10.6 weeks. p,p'-DDE was detected in 64.7% of mothers, at a geometric mean of 0.24 ng/mL. Prenatal p,p´-DDE exposure was not associated with the birth outcomes in the whole sample. However, a high p,p´-DDE exposure was marginally associated with greater small for gestational age risk in male newborns (OR≥0.076ng/mL vs <0.076 ng/mL = 3.09, 95% CI: 0.61; 15.58), but not in female (p for interaction = 0.08).

Even though, we found no reductions in anthropometric measurements or gestational length associated with early prenatal p,p´-DDE exposure, the potential effect modification by infant’s sex in terms of small for the gestational age risk deserves future studies.
Kognitionspsychologie im Fußball
Authors: Lautenbach Franziska , Musculus Lisa , Knöbel Simon , Reinhard Martin Leo , Holst Theresa , Poimann Dino , Pelka Maximilian
Published: May 2022

Zusammenfassung. Anhand des Themas Kognitionspsychologie im Fußball ist es Ziel dieses Beitrags deutlich zu machen, warum es sinnvoll ist, dass Forschung und Praxis der Sportpsychologie und die entsprechenden Akteure integriert und eng zusammenarbeiten. Wir stellen konkrete Überlegungen vor, wie wir diesem Anspruch gerecht werden könnten und nehmen dabei auf ein aktuelles Projekt im Nachwuchsleistungsfußball Bezug. Dabei gehen wir zunächst auf ausgewählte theoretische Grundlagen der Kognitionspsychologie sowie empirische Befunde zur Rolle von Kognitionen und insbesondere der exekutiven Funktionen im Fußball ein, stellen dann Vorteile der Integration von Forschung und Praxis vor, um abschließend konkrete Anstöße zu einer möglichen Umsetzung einer verstärkten Integration von Forschung und Praxis zu geben.
Attributing and Projecting Heatwaves Is Hard: We Can Do Better
Authors: Van Oldenborgh Geert Jan , Wehner Michael F. , Vautard Robert , Otto Friederike E. L. , Seneviratne Sonia I. , Stott Peter A. , Hegerl Gabriele C. , Philip Sjoukje Y. , Kew Sarah F.
Published: May 2022

It sounds straightforward. As the Earth warms due to the increased concentration of greenhouse gases in the atmosphere, global temperatures rise and so heatwaves become warmer as well. This means that a fixed temperature threshold is passed more often: the probability of extreme heat increases. However, land use changes, vegetation change, irrigation, air pollution, and other changes also drive local and regional trends in heatwaves. Sometimes they enhance heatwave intensity, but they can also counteract the effects of climate change, and in some regions, the mechanisms that impact on trends in heatwaves have not yet been fully identified. Climate models simulate heatwaves and the increased intensity and probability of extreme heat reasonably well on large scales. However, changes in annual daily maximum temperatures do not follow global warming over some regions, including the Eastern United States and parts of Asia, reflecting the influence of local drivers as well as natural variability. Also, temperature variability is unrealistic in many models, and can fail standard quality checks. Therefore, reliable attribution and projection of change in heatwaves remain a major scientific challenge in many regions, particularly where the moisture budget is not well simulated, and where land surface changes, changes in short‐lived forcers, and soil moisture interactions are important.
An Online‐Learned Neural Network Chemical Solver for Stable Long‐Term Global Simulations of Atmospheric Chemistry
Authors: Kelp Makoto M. , Jacob Daniel J. , Lin Haipeng , Sulprizio Melissa P.
Published: May 2022

A major computational barrier in global modeling of atmospheric chemistry is the numerical integration of the coupled kinetic equations describing the chemical mechanism. Machine‐learned (ML) solvers can offer order of magnitude speedup relative to conventional implicit solvers but past implementations have suffered from fast error growth and only run for short simulation times (<1 month). A successful ML solver for global models must avoid error growth over yearlong simulations and allow for reinitialization of the chemical trajectory by transport at every time step. Here, we explore the capability of a neural network solver equipped with an autoencoder to achieve stable full‐year simulations of tropospheric oxidant chemistry in the global 3‐D Goddard Earth Observing System (GEOS)‐Chem model, replacing its standard mechanism (228 species) by the Super‐Fast mechanism (12 species) to avoid the curse of dimensionality. We find that online training of the ML solver within GEOS‐Chem is important for accuracy, whereas offline training from archived GEOS‐Chem inputs/outputs produces large errors. After online training, we achieve stable 1‐year simulations with five‐fold speedup compared to the standard implicit Rosenbrock solver with global tropospheric normalized mean biases of −0.3% for ozone, 1% for hydrogen oxide radicals, and −5% for nitrogen oxides. The ML solver captures the diurnal and synoptic variability of surface ozone at polluted and clean sites. There are however large regional biases for ozone and NOx under remote conditions where chemical aging leads to error accumulation. These regional biases remain a major limitation for practical application, and ML emulation would be more difficult in a more complex mechanism.
What next for eating disorder genetics? Replacing myths with facts to sharpen our understanding
Authors: Huckins Laura M. , Signer Rebecca , Johnson Jessica , Wu Ya-Ke , Mitchell Karen S. , Bulik Cynthia M.
Published: May 2022

Substantial progress has been made in the understanding of anorexia nervosa (AN) and eating disorder (ED) genetics through the efforts of large-scale collaborative consortia, yielding the first genome-wide significant loci, AN-associated genes, and insights into metabo-psychiatric underpinnings of the disorders. However, the translatability, generalizability, and reach of these insights are hampered by an overly narrow focus in our research. In particular, stereotypes, myths, assumptions and misconceptions have resulted in incomplete or incorrect understandings of ED presentations and trajectories, and exclusion of certain patient groups from our studies. In this review, we aim to counteract these historical imbalances. Taking as our starting point the Academy for Eating Disorders (AED) Truth #5 “Eating disorders affect people of all genders, ages, races, ethnicities, body shapes and weights, sexual orientations, and socioeconomic statuses”, we discuss what we do and do not know about the genetic underpinnings of EDs among people in each of these groups, and suggest strategies to design more inclusive studies. In the second half of our review, we outline broad strategic goals whereby ED researchers can expand the diversity, insights, and clinical translatability of their studies.
Integrating single-cell RNA sequencing with spatial transcriptomics reveals immune landscape for interstitial cystitis
Authors: Peng Liao , Jin Xi , Li Bo-ya , Zeng Xiao , Liao Bang-hua , Jin Tao , Chen Jia-wei , Gao Xiao-shuai , Wang Wei , He Qing , Chen Guo , Gong Li-Na , Shen Hong , Wang Kun-jie , Li Hong , Luo De-yi
Published: May 2022

Interstitial cystitis (IC) is a severely debilitating and chronic disorder with unclear etiology and pathophysiology, which makes the diagnosis difficult and treatment challenging. To investigate the role of immunity in IC bladders, we sequenced 135,091 CD45+ immune cells from 15 female patients with IC and 9 controls with stress urinary incontinence using single-cell RNA sequencing (scRNA-seq). 22 immune subpopulations were identified in the constructed landscape. Among them, M2-like macrophages, inflammatory CD14+ macrophages, and conventional dendritic cells had the most communications with other immune cells. Then, a significant increase of central memory CD4+ T cells, regulatory T cells, GZMK+CD8+ T cells, activated B cells, un-switched memory B cells, and neutrophils, and a significant decrease of CD8+ effector T cells, Th17 cells, follicular helper T cells, switched memory B cells, transitional B cells, and macrophages were noted in IC bladders. The enrichment analysis identified a virus-related response during the dynamic change of cell proportion, furthermore, the human polyomavirus-2 was detected with a positive rate of 95% in urine of patients with IC. By integrating the results of scRNA-seq with spatial transcriptomics, we found nearly all immune subpopulations were enriched in the urothelial region or located close to fibroblasts in IC bladders, but they were discovered around urothelium and smooth muscle cells in control bladders. These findings depict the immune landscape for IC and might provide valuable insights into the pathophysiology of IC.
Quantitative evaluation of apically extruded debris using TRUShape, TruNatomy, and WaveOne Gold in curved canals
Authors: Roshdy Nehal Nabil , Hassan Reham
Published: May 2022

Objective

This study compared the quantity of extruded debris after instrumentation with TRUShape 3D Conforming files, TruNatomy files, and the WaveOne Gold reciprocating system.

Materials and methods

Fifty-one mesiobuccal canals with severe curvatures (25–40°) were assigned to three equal groups according to the rotary system used for preparation, either TRUShape, TruNatomy, or WaveOne Gold files. The extruded debris was collected in pre-weighed glass vials. The data were statistically analyzed using a one-way ANOVA test and Tukey’s post hoc test.

Results

The least extruded debris was obtained with the WaveOne Gold instruments compared to the TRUShape and TruNatomy files (p < 0.001).

Conclusions

Debris extrusion occurs independently of the motion or design of the instrument. The WaveOne Gold system outperformed TRUShape and TruNatomy files in this study.
Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression
Authors: Ma Luyao , Tian Yeteng , Qian Tao , Li Wenjun , Liu Chengmin , Chu Bizhu , Kong Qian , Cai Renwei , Bai Panzhu , Ma Lisha , Deng Yi , Tian Ruijun , Wu Chuanyue , Sun Ying
Published: May 2022

Androgen receptor (AR) signaling plays important roles in breast cancer progression. We show here that Kindlin-2, a focal adhesion protein, is critically involved in the promotion of AR signaling and breast cancer progression. Kindlin-2 physically associates with AR and Src through its two neighboring domains, namely F1 and F0 domains, resulting in formation of a Kindlin-2-AR-Src supramolecular complex and consequently facilitating Src-mediated AR Tyr-534 phosphorylation and signaling. Depletion of Kindlin-2 was sufficient to suppress Src-mediated AR Tyr-534 phosphorylation and signaling, resulting in diminished breast cancer cell proliferation and migration. Re-expression of wild-type Kindlin-2, but not AR-binding-defective or Src-binding-defective mutant forms of Kindlin-2, in Kindlin-2-deficient cells restored AR Tyr-534 phosphorylation, signaling, breast cancer cell proliferation and migration. Furthermore, re-introduction of phosphor-mimic mutant AR-Y534D, but not wild-type AR reversed Kindlin-2 deficiency-induced inhibition of AR signaling and breast cancer progression. Finally, using a genetic knockout strategy, we show that ablation of Kindlin-2 from mammary tumors in mouse significantly reduced AR Tyr-534 phosphorylation, breast tumor progression and metastasis in vivo. Our results suggest a critical role of Kindlin-2 in promoting breast cancer progression and shed light on the molecular mechanism through which it functions in this process.
Giant converse magnetoelectric effect in a multiferroic heterostructure with polycrystalline Co2FeSi
Authors: Fujii Shumpei , Usami Takamasa , Shiratsuchi Yu , Kerrigan Adam M. , Yatmeidhy Amran Mahfudh , Yamada Shinya , Kanashima Takeshi , Nakatani Ryoichi , Lazarov Vlado K. , Oguchi Tamio , Gohda Yoshihiro , Hamaya Kohei
Published: May 2022

To overcome a bottleneck in spintronic applications such as those of ultralow-power magnetoresistive random-access memory devices, the electric-field control of magnetization vectors in ferromagnetic electrodes has shown much promise. Here, we show the giant converse magnetoelectric (CME) effect in a multiferroic heterostructure consisting of the ferromagnetic Heusler alloy Co2FeSi and ferroelectric-oxide Pb(Mg1/3Nb2/3)O3-PbTiO3 (PMN-PT) for electric-field control of magnetization vectors. Using an in-plane uniaxial magnetic anisotropy of polycrystalline Co2FeSi film grown on PMN-PT(011), the nonvolatile and repeatable magnetization vector switchings in remanent states are demonstrated. The CME coupling coefficient of the polycrystalline Co2FeSi/PMN-PT(011) is over 1.0 × 10−5 s/m at room temperature, comparable to those of single-crystalline Fe1-xGax/PMN-PT systems. The giant CME effect has been demonstrated by the strain-induced variation in the magnetic anisotropy energy of Co2FeSi with an L21-ordered structure. This approach can lead to a new solution to the reduction in the write power in spintronic memory architectures at room temperature.
Modelling armed conflict risk under climate change with machine learning and time-series data
Authors: Ge Quansheng , Hao Mengmeng , Ding Fangyu , Jiang Dong , Scheffran Jürgen , Helman David , Ide Tobias
Published: May 2022

Understanding the risk of armed conflict is essential for promoting peace. Although the relationship between climate variability and armed conflict has been studied by the research community for decades with quantitative and qualitative methods at different spatial and temporal scales, causal linkages at a global scale remain poorly understood. Here we adopt a quantitative modelling framework based on machine learning to infer potential causal linkages from high-frequency time-series data and simulate the risk of armed conflict worldwide from 2000–2015. Our results reveal that the risk of armed conflict is primarily influenced by stable background contexts with complex patterns, followed by climate deviations related covariates. The inferred patterns show that positive temperature deviations or precipitation extremes are associated with increased risk of armed conflict worldwide. Our findings indicate that a better understanding of climate-conflict linkages at the global scale enhances the spatiotemporal modelling capacity for the risk of armed conflict.
Insights into the client protein release mechanism of the ATP-independent chaperone Spy
Authors: He Wei , Li Xinming , Xue Hongjuan , Yang Yuanyuan , Mencius Jun , Bai Ling , Zhang Jiayin , Xu Jianhe , Wu Bin , Xue Yi , Quan Shu
Published: May 2022

Molecular chaperones play a central role in regulating protein homeostasis, and their active forms often contain intrinsically disordered regions (IDRs). However, how IDRs impact chaperone action remains poorly understood. Here, we discover that the disordered N terminus of the prototype chaperone Spy facilitates client release. With NMR spectroscopy and molecular dynamics simulations, we find that the N terminus can bind transiently to the client-binding cavity of Spy primarily through electrostatic interactions mediated by the N-terminal D26 residue. This intramolecular interaction results in a dynamic competition of the N terminus with the client for binding to Spy, which promotes client discharge. Our results reveal the mechanism by which Spy releases clients independent of energy input, thus enriching the current knowledge on how ATP-independent chaperones release their clients and highlighting the importance of synergy between IDRs and structural domains in regulating protein function.
Formation of organic color centers in air-suspended carbon nanotubes using vapor-phase reaction
Authors: Kozawa Daichi , Wu Xiaojian , Ishii Akihiro , Fortner Jacob , Otsuka Keigo , Xiang Rong , Inoue Taiki , Maruyama Shigeo , Wang YuHuang , Kato Yuichiro K.
Published: May 2022

Organic color centers in single-walled carbon nanotubes have demonstrated exceptional ability to generate single photons at room temperature in the telecom range. Combining the color centers with pristine air-suspended nanotubes would be desirable for improved performance, but all current synthetic methods occur in solution which makes them incompatible. Here we demonstrate the formation of color centers in air-suspended nanotubes using a vapor-phase reaction. Functionalization is directly verified by photoluminescence spectroscopy, with unambiguous statistics from more than a few thousand individual nanotubes. The color centers show strong diameter-dependent emission, which can be explained with a model for chemical reactivity considering strain along the tube curvature. We also estimate the defect density by comparing the experiments with simulations based on a one-dimensional exciton diffusion equation. Our results highlight the influence of the nanotube structure on vapor-phase reactivity and emission properties, providing guidelines for the development of high-performance near-infrared quantum light sources.
A pediatric wrist trauma X-ray dataset (GRAZPEDWRI-DX) for machine learning
Authors: Nagy Eszter , Janisch Michael , Hržić Franko , Sorantin Erich , Tschauner Sebastian
Published: May 2022

Digital radiography is widely available and the standard modality in trauma imaging, often enabling to diagnose pediatric wrist fractures. However, image interpretation requires time-consuming specialized training. Due to astonishing progress in computer vision algorithms, automated fracture detection has become a topic of research interest. This paper presents the GRAZPEDWRI-DX dataset containing annotated pediatric trauma wrist radiographs of 6,091 patients, treated at the Department for Pediatric Surgery of the University Hospital Graz between 2008 and 2018. A total number of 10,643 studies (20,327 images) are made available, typically covering posteroanterior and lateral projections. The dataset is annotated with 74,459 image tags and features 67,771 labeled objects. We de-identified all radiographs and converted the DICOM pixel data to 16-Bit grayscale PNG images. The filenames and the accompanying text files provide basic patient information (age, sex). Several pediatric radiologists annotated dataset images by placing lines, bounding boxes, or polygons to mark pathologies like fractures or periosteal reactions. They also tagged general image characteristics. This dataset is publicly available to encourage computer vision research.
Post-resonance backward whirl analysis in cracked overhung rotors
Authors: Alzarooni Tariq , AL-Shudeifat Mohammad , Shiryayev Oleg , Nataraj C.
Published: May 2022

Overhung rotors usually exhibit recurrent transitions through critical whirl rotational speeds during startup and coast down operations, which significantly differ from their steady-state whirl responses. The presence of angular acceleration results in a linear-time-varying (LTV) system, which, although technically linear, still presents complexities often evinced by a nonlinear system. In general, backward whirl zones can either precede the critical forward whirl speed (termed as pre-resonance backward whirl, Pr-BW), or immediately follow the critical forward whirl speed (termed as post-resonance backward whirl, Po-BW). The Po-BW in the whirl response of a cracked overhung rotor with a breathing crack is studied here as distinct from that of geometrically symmetric configurations of other rotor systems. The equations of motion from the finite element (FE) model of an overhung rotor system with a breathing crack are numerically integrated to obtain the whirl response. The transient whirl responses with different bearing conditions are thoroughly investigated for excitation of Po-BW. The Po-BW zones of rotational speeds are determined via the wavelet transform method and full spectrum analysis (FSA) and applied to signals with added noise. The results of this work confirm the excitation of the Po-BW in cracked overhung rotors and confirm the robustness of the employed methods.
Statistical optimization, kinetic, equilibrium isotherm and thermodynamic studies of copper biosorption onto Rosa damascena leaves as a low-cost biosorbent
Authors: Fawzy Mustafa A. , Al-Yasi Hatim M. , Galal Tarek M. , Hamza Reham Z. , Abdelkader Tharwat G. , Ali Esmat F. , Hassan Sedky H. A.
Published: May 2022

In this study, Rosa damascena leaf powder was evaluated as a biosorbent for the removal of copper from aqueous solutions. Process variables such as the biosorbent dose, pH, and initial copper concentration were optimized using response surface methodology. A quadratic model was established to relate the factors to the response based on the Box–Behnken design. Analysis of variance (ANOVA) was used to assess the experimental data, and multiple regression analysis was used to fit it to a second-order polynomial equation. A biosorbent dose of 4.0 g/L, pH of 5.5, and initial copper concentration of 55 mg/L were determined to be the best conditions for copper removal. The removal of Cu2+ ions was 88.7% under these optimal conditions, indicating that the experimental data and model predictions were in good agreement. The biosorption data were well fitted to the pseudo-second-order and Elovich kinetic models. The combination of film and intra-particle diffusion was found to influence Cu2+ biosorption. The Langmuir and Dubinin–Radushkevich isotherm models best fit the experimental data, showing a monolayer isotherm with a qmax value of 25.13 mg/g obtained under optimal conditions. The thermodynamic parameters showed the spontaneity, feasibility and endothermic nature of adsorption. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy were used to characterize the biosorbent before and after Cu2+ biosorption, revealing its outstanding structural characteristics and high surface functional group availability. In addition, immobilized R. damascena leaves adsorbed 90.7% of the copper from aqueous solution, which is more than the amount adsorbed by the free biosorbent (85.3%). The main mechanism of interaction between R. damascena biomass and Cu2+ ions is controlled by both ion exchange and hydrogen bond formation. It can be concluded that R. damascena can be employed as a low-cost biosorbent to remove heavy metals from aqueous solutions.
All-optical manipulation of the Drosophila olfactory system
Authors: Zanon Mirko , Zanini Damiano , Haase Albrecht
Published: May 2022

Thanks to its well-known neuroanatomy, limited brain size, complex behaviour, and the extensive genetic methods,Drosophilahas become an indispensable model in neuroscience. A vast number of studies have focused on its olfactory system and the processing of odour information. Optogenetics is one of the recently developed genetic tools that significantly advance this field of research, allowing to replace odour stimuli by direct neuronal activation with light. This becomes a universal all-optical toolkit when spatially selective optogenetic activation is combined with calcium imaging to read out neuronal responses. Initial experiments showed a successful implementation to study the olfactory system in fish and mice, but the olfactory system ofDrosophilahas been so far precluded from an application. To fill this gap, we present here optogenetic tools to selectively stimulate functional units in theDrosophilaolfactory system, combined with two-photon calcium imaging to read out the activity patterns elicited by these stimuli at different levels of the brain. This method allows to study the spatial and temporal features of the information flow and reveals the functional connectivity in the olfactory network.
Diffuse fibrosis and repolarization disorders explain ventricular arrhythmias in Brugada syndrome: a computational study
Authors: Biasi Niccoló , Seghetti Paolo , Tognetti Alessandro
Published: May 2022

In this work, we reported a computational study to quantitatively determine the individual contributions of three candidate arrhythmic factors associated with Brugada Syndrome. In particular, we focused our analysis on the role of structural abnormalities, dispersion of repolarization, and size of the diseased region. We developed a human phenomenological model capable of replicating the action potential characteristics both in Brugada Syndrome and in healthy conditions. Inspired by physiological observations, we employed the phenomenological model in a 2D geometry resembling the pathological RVOT coupled with healthy epicardial tissue. We assessed the insurgence of sustained reentry as a function of electrophysiological and structural abnormalities. Our computational study indicates that both structural and repolarization abnormalities are essential to induce sustained reentry. Furthermore, our results suggest that neither dispersion of repolarization nor structural abnormalities are sufficient on their own to induce sustained reentry. It should be noted how our study seems to explain an arrhythmic mechanism that unifies the classic repolarization and depolarization hypotheses of the pathophysiology of the Brugada Syndrome. Finally, we believe that this work may offer a new perspective on the computational and clinical investigation of Brugada Syndrome and its arrhythmic behaviour.
Inflammation and remodeling pathways and risk of cardiovascular events in patients with ischemic heart failure and reduced ejection fraction
Authors: Girerd Nicolas , Cleland John , Anker Stefan D. , Byra William , Lam Carolyn S. P. , Lapolice David , Mehra Mandeep R. , van Veldhuisen Dirk J. , Bresso Emmanuel , Lamiral Zohra , Greenberg Barry , Zannad Faiez
Published: May 2022

Patients with heart failure (HF) and coronary artery disease (CAD) have a high risk for cardiovascular (CV) events including HF hospitalization, stroke, myocardial infarction (MI) and sudden cardiac death (SCD). The present study evaluated associations of proteomic biomarkers with CV outcome in patients with CAD and HF with reduced ejection fraction (HFrEF), shortly after a worsening HF episode. We performed a case–control study within the COMMANDER HF international, double-blind, randomized placebo-controlled trial investigating the effects of the factor-Xa inhibitor rivaroxaban. Patients with the following first clinical events: HF hospitalization, SCD and the composite of MI or stroke were matched with corresponding controls for age, sex and study drug. Plasma concentrations of 276 proteins with known associations with CV and cardiometabolic mechanisms were analyzed. Results were corrected for multiple testing using false discovery rate (FDR). In 485 cases and 455 controls, 49 proteins were significantly associated with clinical events of which seven had an adjusted FDR < 0.001 (NT-proBNP, BNP, T-cell immunoglobulin and mucin domain containing 4 (TIMD4), fibroblast growth factor 23 (FGF-23), growth differentiation factor-15 (GDF-15), pulmonary surfactant-associated protein D (PSP-D) and Spondin-1 (SPON1)). No significant interactions were identified between the type of clinical event (MI/stroke, SCD or HFH) and specific biomarkers (all interaction FDR > 0.20). When adding the biomarkers significantly associated with the above outcome to a clinical model (including NT-proBNP), the C-index increase was 0.057 (0.033–0.082), p < 0.0001 and the net reclassification index was 54.9 (42.5 to 67.3), p < 0.0001. In patients with HFrEF and CAD following HF hospitalization, we found that NT-proBNP, BNP, TIMD4, FGF-23, GDF-15, PSP-D and SPON1, biomarkers broadly associated with inflammation and remodeling mechanistic pathways, were strong but indiscriminate predictors of a variety of individual CV events.
Cancer genomic profiling identified dihydropyrimidine dehydrogenase deficiency in bladder cancer promotes sensitivity to gemcitabine
Authors: Tsukahara Shigehiro , Shiota Masaki , Takamatsu Dai , Nagakawa Shohei , Matsumoto Takashi , Kiyokoba Ryo , Yagi Mikako , Setoyama Daiki , Noda Nozomi , Matsumoto Shinya , Hayashi Tetsutaro , Contreras-Sanz Alberto , Black Peter C. , Inokuchi Junichi , Kohashi Kenichi , Oda Yoshinao , Uchiumi Takeshi , Eto Masatoshi , Kang Dongchon
Published: May 2022

Chemotherapy is a standard therapy for muscle-invasive bladder cancer (MIBC). However, genomic alterations associated with chemotherapy sensitivity in MIBC have not been fully explored. This study aimed to investigate the genomic landscape of MIBC in association with the response to chemotherapy and to explore the biological role of genomic alterations. Genomic alterations in MIBC were sequenced by targeted exome sequencing of 409 genes. Gene expression in MIBC tissues was analyzed by western blotting, immunohistochemistry, and RNA microarray. Cellular sensitivity to gemcitabine and gemcitabine metabolite was examined in bladder cancer cells after modulation of candidate gene. Targeted exome sequencing in 20 cases with MIBC revealed various genomic alterations including pathogenic missense mutation of DPYD gene encoding dihydropyrimidine dehydrogenase (DPD). Conversely, high DPYD and DPD expression were associated with poor response to gemcitabine-containing chemotherapy among patients with MIBC, as well as gemcitabine resistance in bladder cancer cells. DPD suppression rendered cells sensitive to gemcitabine, while DPD overexpression made cells gemcitabine-resistant through reduced activity of the cytotoxic gemcitabine metabolite difluorodeoxycytidine diphosphate. This study revealed the novel role of DPD in gemcitabine metabolism. It has been suggested that DPYD genomic alterations and DPD expression are potential predictive biomarkers in gemcitabine treatment.