bluuebunny/crossref_metadata_2025 huggingface dataset:
Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry
Authors: Waller David , Hew Brian , Holdaway Charlie , Jen Michael , Peckham Gabriel
Published: December 2016

Portable detection and quantitation methods for Bacillus anthracis (anthrax) spores in pure culture or in environmental samples are lacking. Here, an amperometric immunoassay has been developed utilizing immunomagnetic separation to capture the spores and remove potential interferents from test samples followed by amperometric measurement on a field-portable instrument. Antibody-conjugated magnetic beads and antibody-conjugated glucose oxidase were used in a sandwich format for the capture and detection of target spores. Glucose oxidase activity of spore pellets was measured indirectly via amperometry by applying a bias voltage after incubation with glucose, horseradish peroxidase, and the electron mediator 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid). Target capture was mediated by polyclonal antisera, whereas monoclonal antibodies were used for signal generation. This strategy maximized sensitivity (500 target spores, 5000 cfu/mL), while also providing a good specificity for Bacillus anthracis spores. Minimal signal deviation occurs in the presence of environmental interferents including soil and modified pH conditions, demonstrating the strengths of immunomagnetic separation. The simultaneous incubation of capture and detection antibodies and rapid substrate development (5 min) result in short sample-to-signal times (less than an hour). With attributes comparable or exceeding that of ELISA and LFDs, amperometry is a low-cost, low-weight, and practical method for detecting anthrax spores in the field.
Versatile Flexible Graphene Multielectrode Arrays
Authors: Kireev Dmitry , Seyock Silke , Ernst Mathis , Maybeck Vanessa , Wolfrum Bernhard , Offenhäusser Andreas
Published: December 2016

Graphene is a promising material possessing features relevant to bioelectronics applications. Graphene microelectrodes (GMEAs), which are fabricated in a dense array on a flexible polyimide substrate, were investigated in this work for their performance via electrical impedance spectroscopy. Biocompatibility and suitability of the GMEAs for extracellular recordings were tested by measuring electrical activities from acute heart tissue and cardiac muscle cells. The recordings show encouraging signal-to-noise ratios of 65 ± 15 for heart tissue recordings and 20 ± 10 for HL-1 cells. Considering the low noise and excellent robustness of the devices, the sensor arrays are suitable for diverse and biologically relevant applications.
Preconditioning of Spatial and Auditory Cues: Roles of the Hippocampus, Frontal Cortex, and Cue-Directed Attention
Authors: Talk Andrew , Grasby Katrina , Rawson Tim , Ebejer Jane
Published: December 2016

Loss of function of the hippocampus or frontal cortex is associated with reduced performance on memory tasks, in which subjects are incidentally exposed to cues at specific places in the environment and are subsequently asked to recollect the location at which the cue was experienced. Here, we examined the roles of the rodent hippocampus and frontal cortex in cue-directed attention during encoding of memory for the location of a single incidentally experienced cue. During a spatial sensory preconditioning task, rats explored an elevated platform while an auditory cue was incidentally presented at one corner. The opposite corner acted as an unpaired control location. The rats demonstrated recollection of location by avoiding the paired corner after the auditory cue was in turn paired with shock. Damage to either the dorsal hippocampus or the frontal cortex impaired this memory ability. However, we also found that hippocampal lesions enhanced attention directed towards the cue during the encoding phase, while frontal cortical lesions reduced cue-directed attention. These results suggest that the deficit in spatial sensory preconditioning caused by frontal cortical damage may be mediated by inattention to the location of cues during the latent encoding phase, while deficits following hippocampal damage must be related to other mechanisms such as generation of neural plasticity.
Genetic Pathways to Insomnia
Authors: Lind Mackenzie , Gehrman Philip
Published: December 2016

This review summarizes current research on the genetics of insomnia, as genetic contributions are thought to be important for insomnia etiology. We begin by providing an overview of genetic methods (both quantitative and measured gene), followed by a discussion of the insomnia genetics literature with regard to each of the following common methodologies: twin and family studies, candidate gene studies, and genome-wide association studies (GWAS). Next, we summarize the most recent gene identification efforts (primarily GWAS results) and propose several potential mechanisms through which identified genes may contribute to the disorder. Finally, we discuss new genetic approaches and how these may prove useful for insomnia, proposing an agenda for future insomnia genetics research.
Impact of Spinal Manipulation on Cortical Drive to Upper and Lower Limb Muscles
Authors: Haavik Heidi , Niazi Imran , Jochumsen Mads , Sherwin Diane , Flavel Stanley , Türker Kemal
Published: December 2016

This study investigates whether spinal manipulation leads to changes in motor control by measuring the recruitment pattern of motor units in both an upper and lower limb muscle and to see whether such changes may at least in part occur at the cortical level by recording movement related cortical potential (MRCP) amplitudes. In experiment one, transcranial magnetic stimulation input–output (TMS I/O) curves for an upper limb muscle (abductor pollicus brevis; APB) were recorded, along with F waves before and after either spinal manipulation or a control intervention for the same subjects on two different days. During two separate days, lower limb TMS I/O curves and MRCPs were recorded from tibialis anterior muscle (TA) pre and post spinal manipulation. Dependent measures were compared with repeated measures analysis of variance, with p set at 0.05. Spinal manipulation resulted in a 54.5% ± 93.1% increase in maximum motor evoked potential (MEPmax) for APB and a 44.6% ± 69.6% increase in MEPmax for TA. For the MRCP data following spinal manipulation there were significant difference for amplitude of early bereitschafts-potential (EBP), late bereitschafts potential (LBP) and also for peak negativity (PN). The results of this study show that spinal manipulation leads to changes in cortical excitability, as measured by significantly larger MEPmax for TMS induced input–output curves for both an upper and lower limb muscle, and with larger amplitudes of MRCP component post manipulation. No changes in spinal measures (i.e., F wave amplitudes or persistence) were observed, and no changes were shown following the control condition. These results are consistent with previous findings that have suggested increases in strength following spinal manipulation were due to descending cortical drive and could not be explained by changes at the level of the spinal cord. Spinal manipulation may therefore be indicated for the patients who have lost tonus of their muscle and/or are recovering from muscle degrading dysfunctions such as stroke or orthopaedic operations and/or may also be of interest to sports performers. These findings should be followed up in the relevant populations.
Assessment of the Impact of Cool Roofs in Temperate Climates through a Comparative Experimental Campaign in Outdoor Test Cells
Authors: Barozzi Benedetta , Pollastro M.
Published: December 2016

Over the last few decades many bibliographical studies have been conducted on cool roofs, by analyzing both the energy performance during summer and winter seasons and their contribution in mitigating the urban heat island effect. This paper describes the experimentation carried out in three outdoor test cells to comparatively assess the behavior of a cool roof solution with respect to a ventilated-roof and a warm-roof. The monitored data have confirmed that in summer cool roofs are the best solution, with respect to traditional tile roofing, sensibly reducing surface temperatures as a function of the solar direct radiation level (15–25 °C for I > 600 W/m2 and 5–15 °C for I < 300 W/m2, independently of the season). Furthermore, cool roofs involve an energy saving related to summer cooling by about 20% as to a warm-roof and 15%–20% as to a vented-roof. During winter, cool roofs improve the heating energy performance of a vented-roof by about 12%, against a slightly increased consumption (<5%) if compared to a warm-roof. These data confirm that, in temperate climates, cool roofs are appropriate solutions to sensibly improve summer energy performances with a negligible negative impact during winter, and to positively contribute to the mitigation of the heat island effect.
CO2 Adsorption by para-Nitroaniline Sulfuric Acid-Derived Porous Carbon Foam
Authors: Andreoli Enrico , Barron Andrew
Published: December 2016

The expansion product from the sulfuric acid dehydration of para-nitroaniline has been characterized and studied for CO2 adsorption. The X-ray photoelectron spectroscopy (XPS) characterization of the foam indicates that both N and S contents (15 and 9 wt%, respectively) are comparable to those separately reported for nitrogen- or sulfur-containing porous carbon materials. The analysis of the XPS signals of C1s, O1s, N1s, and S2p reveals the presence of a large number of functional groups and chemical species. The CO2 adsorption capacity of the foam is 7.9 wt% (1.79 mmol/g) at 24.5 °C and 1 atm in 30 min, while the integral molar heat of adsorption is 113.6 kJ/mol, indicative of the fact that chemical reactions characteristic of amine sorbents are observed for this type of carbon foam. The kinetics of adsorption is of pseudo-first-order with an extrapolated activation energy of 18.3 kJ/mol comparable to that of amine-modified nanocarbons. The richness in functionalities of H2SO4-expanded foams represents a valuable and further pursuable approach to porous carbons alternative to KOH-derived activated carbons.
Pain Neuroscience Education: State of the Art and Application in Pediatrics
Authors: Robins Hannah , Perron Victoria , Heathcote Lauren , Simons Laura
Published: December 2016

Chronic pain is a widespread problem in the field of pediatrics. Many interventions to ameliorate pain-related dysfunction have a biobehavioral focus. As treatments for chronic pain (e.g., increased movement) often stand in stark contrast to treatments for an acute injury (e.g., rest), providing a solid rationale for treatment is necessary to gain patient and parent buy-in. Most pain treatment interventions incorporate psychoeducation, or pain neuroscience education (PNE), as an essential component, and in some cases, as a stand-alone approach. The current topical review focuses on the state of pain neuroscience education and its application to pediatric chronic pain. As very little research has examined pain neuroscience education in pediatrics, we aim to describe this emerging area and catalyze further work on this important topic. As the present literature has generally focused on adults with chronic pain, pain neuroscience education merits further attention in the realm of pediatric pain in order to be tailored and implemented in this population.
Acute Diarrhoea in Children: Determination of Duration Using a Combined Bismuth Hydroxide Gel and Oral Rehydration Solution Therapy vs. Oral Rehydration Solution
Authors: Oviedo Adriana , Díaz Mirna , Valenzuela María , Vidal Victoria , Racca Liliana , Bottai Hebe , Priore Graciela , Peluffo Graciela , Di Bartolomeo Susana , Cabral Graciela , Toca María del Carmen
Published: December 2016

Oral rehydration salt (ORS) treatment in young children with acute diarrhoea (AD) has contributed to decrease mortality associated with dehydration although effective strategies to reduce morbidity associated with this disease are required. The aim of this study was to evaluate the diarrhoea duration when using combined colloidal bismuth hydroxide gel (CBHG) and oral rehydration salt treatment compared with ORS therapy in children with AD. We designed a double-blind, randomised prospective study with treatment and control groups. Patients aged one to 12 years, with no prior pathology and with AD of less than 48 h were included. The Chi-squared and Mann-Whitney tests were used, as well as the Cox proportional hazards model and the Kaplan-Meier estimator. Patients were randomised into an ORS and CBHG treatment group and a control group for ORS plus placebo. (Average age: 3.2 years). The result of the post-treatment evaluation with respect to the average duration of AD was 25.5 h for the treated group vs. 41.5 h for the control group (p = 0.015). The average number of stools was 4.8 in the treated group and 8.2 in the control group (p = 0.032). We conclude that the use of CBHG plus ORS significantly reduced the duration of AD, the number of stools and the percentage of children with persistent AD after 24 h of treatment compared to the control group. AD remitted almost twice as fast in patients treated with CBHG and ORS compared to those who received ORS plus placebo.
Flood in a Changing Climate: The Impact on Livelihood and How the Rural Poor Cope in Bangladesh
Authors: Parvin Gulsan , Shimi Annya , Shaw Rajib , Biswas Chaitee
Published: December 2016

It is already documented that climate change will lead to an intensification of the global water cycle with a consequent increase in flood hazards. Bangladesh is also facing an increasing trend of flood disasters. Among the various risks and disasters in Bangladesh, flood is the most common and frequent. Floods make people vulnerable, as they take away their livelihoods at the first instance and leave them with little resources to overcome from the situation. Because of floods, rural poor communities face job loss, and two-thirds of their income is reduced, which limits their capabilities of preparedness, response, and recovery to subsequent floods. People cope with the situation by bearing substantial debts and a loss of productive assets. With an empirical field study in one of the most flood-prone upazilas (sub-districts) of Bangladesh, namely Goalanda Upazilla of the Rajbari district, this study intends to draw a “flood impact tree” of the study area. It also examines the impacts of flood on the livelihood of the rural poor and explores their coping strategies. This paper aims to facilitate an understanding of the impact of floods on their livelihood, especially on the income and occupations of the rural poor. At the same time, it aims to learn from their coping mechanisms.
Local Electrical Response in Alkaline-Doped Electrodeposited CuInSe2/Cu Films
Authors: Barón-Miranda Javier , Calzadilla Octavio , Arvizu-Rodríguez Liliana , Fernández-Muñoz Jose , Guarneros-Aguilar Cesia , Chale-Lara Fabio , Páramo-García Ulises , Caballero-Briones Felipe
Published: December 2016

The local electrical response in alkaline-doped CuInSe2 films prepared by single-step electrodeposition onto Cu substrates was studied by current-sensing atomic force microscopy. The CuInSe2 (CIS) films were prepared from single baths containing the dopant ions (Li, Na, K or Cs) and were studied by X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectroscopy and photocurrent response. Increased crystallinity and surface texturing as the ion size increased were observed, as well as an enhanced photocurrent response in Cs-doped CIS. Li- and Na-doped films had larger conductivity than the undoped film while the K- and Cs-doped samples displayed shorter currents and the current images indicated strong charge accumulation in the K- and Cs-doped films, forming surface capacitors. Corrected current-sensing AFM IV curves were adjusted with the Shockley equation.
Genetics and Conservation of Plant Species of Extremely Narrow Geographic Range
Authors: Solórzano Sofia , Arias Salvador , Dávila Patricia
Published: December 2016

The endemic plant species with extremely narrow geographical range (<100 km2) often have few populations of small size and tend to be more vulnerable to extinction by genetic drift and inbreeding effects. For these species, we tested if intraspecific genetic diversity can be applied to identify conservation priorities. The biological model was Mammillaria albiflora—a Mexican cactus that numbers ~1000 individuals distributed in four nearby patches covering 4.3 km2. A total of 96 individuals were genotyped with 10 microsatellite loci to describe the genetic substructure and diversity. There is significant population substructure: the genetic diversity is distributed in three genetic neighbors and varies among the patches, the genotypes are not randomly distributed and three genetic barriers restrict the gene flow. The current population size is 15 times smaller than in the past. The restricted gene flow and genetic drift are the processes that have shaped population substructure. To conserve the genetic diversity of this cactus we recommend that two patches, which are not private property, be legally protected; to include M. albiflora in the Red List Species of Mexico in the category of extinction risk; and a legal propagation program may help to diminish the illegal harvesting.
Oral Biofluid Biomarker Research: Current Status and Emerging Frontiers
Authors: Wang Austin , Wang Chris , Tu Michael , Wong David
Published: December 2016

Salivary diagnostics is a rapidly advancing field that offers clinicians and patients the potential of rapid, noninvasive diagnostics with excellent accuracy. In order for the complete realization of the potential of saliva, however, extensive profiling of constituents must be conducted and diagnostic biomarkers must be thoroughly validated. This article briefly overviews the process of conducting a study of salivary biomarkers in a patient cohort and highlights the studies that have been conducted on different classes of molecules in the saliva. Emerging frontiers in salivary diagnostics research that may significantly advance the field will also be highlighted.
The Cardiovascular Effects of Cocoa Polyphenols—An Overview
Authors: Aprotosoaie Ana , Miron Anca , Trifan Adriana , Luca Vlad , Costache Irina-Iuliana
Published: December 2016

Cocoa is a rich source of high-quality antioxidant polyphenols. They comprise mainly catechins (29%–38% of total polyphenols), anthocyanins (4% of total polyphenols) and proanthocyanidins (58%–65% of total polyphenols). A growing body of experimental and epidemiological evidence highlights that the intake of cocoa polyphenols may reduce the risk of cardiovascular events. Beyond antioxidant properties, cocoa polyphenols exert blood pressure lowering activity, antiplatelet, anti-inflammatory, metabolic and anti-atherosclerotic effects, and also improve endothelial function. This paper reviews the role of cocoa polyphenols in cardiovascular protection, with a special focus on mechanisms of action, clinical relevance and correlation between antioxidant activity and cardiovascular health.
Entropic Citizenship Behavior and Sustainability in Urban Organizations: Towards a Theoretical Model
Authors: Coldwell David
Published: December 2016

Entropy is a concept derived from Physics that has been used to describe natural and social systems’ structure and behavior. Applications of the concept in the social sciences so far have been largely limited to the disciplines of economics and sociology. In the current paper, the concept of entropy is applied to organizational citizenship behavior with implications for urban organizational sustainability. A heuristic is presented for analysing personal and organizational citizenship configurations and distributions within a given workforce that can lead to corporate entropy; and for allowing prescriptive remedial steps to be taken to manage the process, should entropy from this source threaten its sustainability and survival.
A Possible Application of the Contribution of Aromaticity to Entropy: Thermal Switch
Authors: Coustel Romain , Carniato Stéphane , Boureau Gérard
Published: December 2016

It has been known for a long time that the loss of aromaticity of gaseous molecules leads to a large increase of the enthalpy and to a tiny increase of the entropy. Generally, the calculated transition temperature from an aromatic structure towards a non-aromatic structure at which these two contributions cancel is very high. The entropy associated to the loss of aromaticity of adsorbed molecules, such as pyridine on Si(100) and on Ge(100), is roughly the same while the associated enthalpy is much smaller, a consequence of which is a low transition temperature. This allows us to imagine monomolecular devices, such as thermal switches, based on the difference of electrical conductivity between aromatic and non-aromatic species adsorbed on Si(100) or on Ge(100).
Monitoring Test for Stability of Dependence Structure in Multivariate Data Based on Copula
Authors: Lee Jiyeon , Kim Byungsoo
Published: December 2016

In this paper, we consider a sequential monitoring procedure for detecting changes in copula function. We propose a cusum type of monitoring test based on the empirical copula function and apply it to the detection of the distributional changes in copula function. We investigate the asymptotic properties of the stopping time and show that under regularity conditions, its limiting null distribution is the same as the sup of Kiefer process. Moreover, we utilize the bootstrap method in order to obtain the limiting distribution. A simulation study and a real data analysis are conducted to evaluate our test.
Echo State Condition at the Critical Point
Authors: Mayer Norbert
Published: December 2016

Recurrent networks with transfer functions that fulfil the Lipschitz continuity with K = 1 may be echo state networks if certain limitations on the recurrent connectivity are applied. It has been shown that it is sufficient if the largest singular value of the recurrent connectivity is smaller than 1. The main achievement of this paper is a proof under which conditions the network is an echo state network even if the largest singular value is one. It turns out that in this critical case the exact shape of the transfer function plays a decisive role in determining whether the network still fulfills the echo state condition. In addition, several examples with one-neuron networks are outlined to illustrate effects of critical connectivity. Moreover, within the manuscript a mathematical definition for a critical echo state network is suggested.
Quantum Key Distribution in the Presence of the Intercept-Resend with Faked States Attack
Authors: Lizama-Pérez Luis , López José , De Carlos López Eduardo
Published: December 2016

Despite the unconditionally secure theory of the Quantum Key Distribution (Q K D), several attacks have been successfully implemented against commercial Q K D systems. Those systems have exhibited some flaws, as the secret key rate of corresponding protocols remains unaltered, while the eavesdropper obtains the entire secret key. We propose the negative acknowledgment state quantum key distribution protocol as a novel protocol capable of detecting the eavesdropping activity of the Intercept Resend with Faked Sates (I R F S) attack without requiring additional optical components different from the B B 84 protocol because the system can be implemented as a high software module. In this approach, the transmitter interleaves pairs of quantum states, referred to here as parallel and orthogonal states, while the receiver uses active basis selection.
A New Dynamic Injection System of Urea-Water Solution for a Vehicular Select Catalyst Reduction System
Authors: Li Long , Lin Wei , Zhang Youtong
Published: December 2016

Since the Euro-ІІІ standard was adopted, the main methods to inhibit NOx production in diesel engines are exhaust gas recirculation (EGR) and select catalyst reduction (SCR). On these methods SCR offers great fuel economy, so it has received wide attention. However, there also exists a trade-off law between NOx conversion efficiency and NH3 slip under dynamic conditions. To inhibit NH3 slip with high NOx conversion efficiency, a dynamic control method for a urea water solution (UWS) injection was investigated. The variation phenomena of SCR conversion efficiency with respect to the cross-sensitivity characteristics of the NOx sensor to NH3 have been thoroughly analyzed. The methodology of “uncertain conversion efficiency curve tangent analysis” has been applied to estimate the concentration of the slipped NH3. The correction factor “φ” of UWS injection is obtained by a comparative calculation of the NOx conversion ability and subsequent NH3 slip. It also includes methods of flow compensation and flow reduction. The proposed control method has been authenticated under dynamic conditions. In low frequency dynamic experiments, this control method has accurately justified the NH3 slip process and inhibits the NH3 emission to a lower level thereby improving the conversion efficiency to a value closer to the target value. The results of European transient cycle (ETC) experiments indicate that NH3 emissions are reduced by 90.8% and the emission level of NOx is close to the Euro-Ѵ standard.
Thermo-Economic Comparison and Parametric Optimizations among Two Compressed Air Energy Storage System Based on Kalina Cycle and ORC
Authors: Li Ruixiong , Wang Huanran , Yao Erren , Zhang Shuyu
Published: December 2016

The compressed air energy storage (CAES) system, considered as one method for peaking shaving and load-levelling of the electricity system, has excellent characteristics of energy storage and utilization. However, due to the waste heat existing in compressed air during the charge stage and exhaust gas during the discharge stage, the efficient operation of the conventional CAES system has been greatly restricted. The Kalina cycle (KC) and organic Rankine cycle (ORC) have been proven to be two worthwhile technologies to fulfill the different residual heat recovery for energy systems. To capture and reuse the waste heat from the CAES system, two systems (the CAES system combined with KC and ORC, respectively) are proposed in this paper. The sensitivity analysis shows the effect of the compression ratio and the temperature of the exhaust on the system performance: the KC-CAES system can achieve more efficient operation than the ORC-CAES system under the same temperature of exhaust gas; meanwhile, the larger compression ratio can lead to the higher efficiency for the KC-CAES system than that of ORC-CAES with the constant temperature of the exhaust gas. In addition, the evolutionary multi-objective algorithm is conducted between the thermodynamic and economic performances to find the optimal parameters of the two systems. The optimum results indicate that the solutions with an exergy efficiency of around 59.74% and 53.56% are promising for KC-CAES and ORC-CAES system practical designs, respectively.
The Effect of Two Types of Biochars on the Efficacy, Emission, Degradation, and Adsorption of the Fumigant Methyl Isothiocyanate
Authors: Fang Wensheng , Cao Aocheng , Yan Dongdong , Han Dawei , Huang Bin , Li Jun , Liu Xiaoman , Guo Meixia , Wang Qiuxia
Published: December 2016

Biochar (BC) is increasingly applied in agriculture; however, due to its adsorption and degradation properties, biochar may also affect the efficacy of fumigant in amended soil. Our research is intended to study the effects of two types of biochars (BC-1 and BC-2) on the efficacy and emission of methyl isothiocyanate (MITC) in biochar amendment soil. Both types of biochars can significantly reduce MITC emission losses, but, at the same time, decrease the concentration of MITC in the soil. The efficacy of MITC for controlling soil-borne pests (Meloidogyne spp., Fusarium spp. Phytophthora spp., Abutilon theophrasti and Digitaria sanguinalis) was reduced when the biochar (BC-1 and BC-2) was applied at a rate of higher than 1% and 0.5% (on a weight basis) (on a weight basis), respectively. However, increased doses of dazomet (DZ) were able to offset decreases in the efficacy of MITC in soils amended with biochars. Biochars with strong adsorption capacity (such as BC-1) substantially reduced MITC degradation rate by 6.2 times, and increased by 4.1 times following amendment with biochar with high degradability (e.g., BC-2), compared to soil without biochar amendment. This is due to the adsorption and degradation of biochar that reduces MITC emission losses and pest control.
Modeling of a Field-Modulated Permanent-Magnet Machine
Authors: Li Xianglin , Chau K. , Wang Yubin
Published: December 2016

In this work, an effective field-modulated permanent-magnet (FMPM) machine was investigated, in which the spoke-magnet outer rotor and open-slot stator were employed. The objective of this paper is to provide the mathematical modeling analysis that was performed for the purpose of control research on this type of FMPM machine. The simulation results by means of finite element analysis (FEA) are given to verify the theoretical analysis and the validity of mathematical model. A prototype machine was also fabricated for experimentation. Both the analytical model and the FEA results are validated by experimental tests on the prototype machine.
Design of an Energy Efficient Future Base Station with Large-Scale Antenna System
Authors: Lee Byung , Kim Youngok
Published: December 2016

Due to the continuous increase in data demanded by end-users, an energy-efficient base station (BS) is a vital topic of interest that would not only result in a substantial economic impact on service providers, but would also reduce the carbon footprint of operating a network. In this regard, we propose the structure and systematic operation of a BS with a large-scale (LS) antenna system that can increase the energy efficiency (EE) of cellular systems. The proposed BS structure includes various power-related units, such as a central management apparatus, power controller, EE calculator, radio site-dependent parameter space (RSD-PS) and determiner. With the information provided from each unit, the decision unit determines how to adjust each component of the BS in order to maximize the EE. Extensive simulations show that the proposed BS improves the EE performance by about 83.05% relative to the reference BS.
Sensorless Control of Interior Permanent Magnet Synchronous Motor in Low-Speed Region Using Novel Adaptive Filter
Authors: Tian Lisi , Zhao Jin , Sun Jiajiang
Published: December 2016

This paper presents a novel position and speed estimation method for low-speed sensorless control of interior permanent-magnet synchronous machines (IPMSMs). The parameter design of the position and speed estimator is based on the sampled current rather than the motor electrical parameters. The proposed method not only simplifies the parameter design, it enables the estimator to work normally even in the condition that the electrical parameters are uncertain or varied. The adaptive filters are adopted to extract the desired high frequency current. The structure and corresponding transfer function are analyzed. To address the shortage of insufficient stop-band attenuation, the structure of the adaptive filter is modified to provide suitable bandwidth and stop-band attenuation simultaneously. The effectiveness of the proposed sensorless control strategy has been verified by simulations and experiments.
Systematic Assessment of Carbon Emissions from Renewable Energy Access to Improve Rural Livelihoods
Authors: Cherni Judith , Olalde Font Raúl , Serrano Lucía , Henao Felipe , Urbina Antonio
Published: December 2016

One way of increasing access to electricity for impoverished unconnected areas without adding significant amounts of CO2 to the atmosphere is by promoting renewable energy technologies. However, decision-makers rarely, if ever, take into account the level of in-built energy requirements and consequential CO2 emissions found in renewable energy, particularly photovoltaic cells and related equipment, which have been widely disseminated in developing countries. The deployment of solar panels worldwide has mostly relied on silicon crystalline cell modules, despite the fact that less polluting material—in particular, thin film and organic cells—offers comparatively distinct technical, environmental and cost advantages characteristics. A major scientific challenge has thus been the design of a single decision-making approach to assess local and global climate change-related impacts as well as the socio-economic effects of low-carbon technology. The article focuses on the functions of the multi-criteria-based tool SURE-DSS and environmental impact analysis focused on greenhouse gases (GHG) emissions balance to inform the selection of technologies in terms of their impact on livelihoods and CO2eq. emissions. An application in a remote rural community in Cuba is discussed. The results of this study show that while PV silicon (c-Si), thin film (CdTe) and organic solar cells may each equally meet the demands of the community and enhance people’s livelihoods, their effect on the global environment varies.
Analyzing Crude Oil Spot Price Dynamics versus Long Term Future Prices: A Wavelet Analysis Approach
Authors: Polanco-Martínez Josué , Abadie Luis
Published: December 2016

The West Texas Intermediate (WTI) spot price shows high volatility and in 2014 and 2015 when quoted prices declined sharply, long-term prices in future markets were less volatile. These prices are different and diverge depending on how they process fundamental and transitory factors. US tight oil production has been a major innovation with significant macroeconomic effects. In this paper we use WTI spot prices and long-term futures prices, the latter calculated as the expected value with a stochastic model calibrated with the futures quotes of each sample day. These long-term prices are the long-term equilibrium value under risk neutral measurement. In order to analyze potential time-scale relationships between spots and future, we perform a wavelet cross-correlation analysis using a novel wavelet graphical tool recently proposed. To check the direction of the causality, we apply non-linear causality tests to raw data and log returns as well as to the wavelet transform of the spot and futures prices. Our results show that in the spot and futures markets for the period 24 February 2006–2 April 2016 there is a bi-directional causality effect for most time scales (from intra-week to biannual). This suggests that spot and futures prices react simultaneously to new information.
A Comparison of Modulation Techniques for Modular Multilevel Converters
Authors: Moranchel Miguel , Huerta Francisco , Sanz Inés , Bueno Emilio , Rodríguez Francisco
Published: December 2016

This work presents a comparison of three different modulation techniques applied to modular multilevel converters (MMCs). The three modulation strategies studied in this paper are the phase-shifted sinusoidal pulse width modulation (PS-SPWM), the space-vector modulation (SVM) and the nearest level modulation (NLM). This paper focuses on analysing the particularities and implementation of each modulation technique. The modulation technique largely defines the generated harmonic content, making this is a key point that must be studied in depth. The paper briefly describes the three modulation techniques and analyses the harmonics generated by each one of the methods. In addition, the paper presents and compares the digital implementation of the three modulation methods in a Field Programmable Gate Array (FPGA). The proposed approaches are validated using a real processing platform and experimentally evaluated in a real high-power six-level MMC.
Identification and Target Prediction of MicroRNAs in Ulmus pumila L. Seedling Roots under Salt Stress by High-Throughput Sequencing
Authors: Zhu Jianfeng , Yang Xiuyan , Liu Zhengxiang , Zhang Huaxin
Published: December 2016

MicroRNAs (miRNAs) are a class of endogenous small RNAs with important roles in plant growth, development, and environmental stress responses. Ulmus pumila L., a deciduous broadleaved tree species of northern temperate regions, is widely distributed in central and northern Asia and has important economic and ecological value. With the spread and aggravation of soil salinization, salt stress has become a major abiotic stress affecting the normal growth and development of U. pumila. However, the influence of salt stress on U. pumila miRNA expression has not been investigated. To identify miRNAs and predict their target mRNA genes under salt stress, three small RNA libraries were generated and sequenced from roots of U. pumila seedlings treated with various concentrations of NaCl corresponding to no salt stress, light short-term salt stress, and medium-heavy long-term salt stress. Integrative analysis identified 254 conserved miRNAs representing 29 families and 49 novel miRNAs; 232 potential targets of the miRNAs were also predicted. Expression profiling of miRNAs between libraries was performed, and the expression of six miRNAs was validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Our findings provide an overview of potential miRNAs and corresponding targets involved in regulating U. pumila salt defense responses. These results lay the foundation for further research into molecular mechanisms involved in salt stress resistance in U. pumila and other Ulmaceae species.
Mineral Nutritional Yield and Nutrient Density of Locally Adapted Wheat Genotypes under Organic Production
Authors: Moreira-Ascarrunz Sergio , Larsson Hans , Prieto-Linde Maria , Johansson Eva
Published: December 2016

The aim of the present investigation was to investigate the nutritional yield, nutrient density, stability, and adaptability of organically produced wheat for sustainable and nutritional high value food production. This study evaluated the nutritional yield of four minerals (Fe, Zn, Cu, and Mg) in 19 wheat genotypes, selected as being locally adapted under organic agriculture conditions. The new metric of nutritional yield was calculated for each genotype and they were evaluated for stability using the Additive Main effects and Multiplicative Interaction (AMMI) stability analysis and for genotypic value, stability, and adaptability using the Best Linear Unbiased Prediction (BLUP procedure). The results indicated that there were genotypes suitable for production under organic agriculture conditions with satisfactory yields (>4000 kg·ha−1). Furthermore, these genotypes showed high nutritional yield and nutrient density for the four minerals studied. Additionally, since these genotypes were stable and adaptable over three environmentally different years, they were designated “balanced genotypes” for the four minerals and for the aforementioned characteristics. Selection and breeding of such “balanced genotypes” may offer an alternative to producing nutritious food under low-input agriculture conditions. Furthermore, the type of evaluation presented here may also be of interest for implementation in research conducted in developing countries, following the objectives of producing enough nutrients for a growing population.
Using the Outskirts of Galaxy Clusters to Determine Their Mass Accretion Rate
Authors: De Boni Cristiano
Published: December 2016

We explore the possibility of using the external regions of galaxy clusters to measure their mass accretion rate (MAR). The main goal is to provide a method to observationally investigate the growth of structures on the nonlinear scales of galaxy clusters. We derive the MAR by using the mass profile beyond the splashback radius, evaluating the mass of a spherical shell and the time it takes to fall in. The infall velocity of the shell is extracted from N-body simulations. The average MAR returned by our prescription in the redshift range z = [ 0 , 2 ] is within 20%–40% of the average MAR derived from the merger trees of dark matter haloes in the reference N-body simulations. Our result suggests that the external regions of galaxy clusters can be used to measure the mean MAR of a sample of clusters.
Hybridization Capture-Based Next-Generation Sequencing to Evaluate Coding Sequence and Deep Intronic Mutations in the NF1 Gene
Authors: Cunha Karin , Oliveira Nathalia , Fausto Anna , De Souza Carolina , Gros Audrey , Bandres Thomas , Idrissi Yamina , Merlio Jean-Philippe , De Moura Neto Rodrigo , Silva Rosane , Geller Mauro , Cappellen David
Published: December 2016

Neurofibromatosis 1 (NF1) is one of the most common genetic disorders and is caused by mutations in the NF1 gene. NF1 gene mutational analysis presents a considerable challenge because of its large size, existence of highly homologous pseudogenes located throughout the human genome, absence of mutational hotspots, and diversity of mutations types, including deep intronic splicing mutations. We aimed to evaluate the use of hybridization capture-based next-generation sequencing to screen coding and noncoding NF1 regions. Hybridization capture-based next-generation sequencing, with genomic DNA as starting material, was used to sequence the whole NF1 gene (exons and introns) from 11 unrelated individuals and 1 relative, who all had NF1. All of them met the NF1 clinical diagnostic criteria. We showed a mutation detection rate of 91% (10 out of 11). We identified eight recurrent and two novel mutations, which were all confirmed by Sanger methodology. In the Sanger sequencing confirmation, we also included another three relatives with NF1. Splicing alterations accounted for 50% of the mutations. One of them was caused by a deep intronic mutation (c.1260 + 1604A > G). Frameshift truncation and missense mutations corresponded to 30% and 20% of the pathogenic variants, respectively. In conclusion, we show the use of a simple and fast approach to screen, at once, the entire NF1 gene (exons and introns) for different types of pathogenic variations, including the deep intronic splicing mutations.
The Causes and Consequences of Topological Stress during DNA Replication
Authors: Keszthelyi Andrea , Minchell Nicola , Baxter Jonathan
Published: December 2016

The faithful replication of sister chromatids is essential for genomic integrity in every cell division. The replication machinery must overcome numerous difficulties in every round of replication, including DNA topological stress. Topological stress arises due to the double-stranded helical nature of DNA. When the strands are pulled apart for replication to occur, the intertwining of the double helix must also be resolved or topological stress will arise. This intrinsic problem is exacerbated by specific chromosomal contexts encountered during DNA replication. The convergence of two replicons during termination, the presence of stable protein-DNA complexes and active transcription can all lead to topological stresses being imposed upon DNA replication. Here we describe how replication forks respond to topological stress by replication fork rotation and fork reversal. We also discuss the genomic contexts where topological stress is likely to occur in eukaryotes, focusing on the contribution of transcription. Finally, we describe how topological stress, and the ways forks respond to it, may contribute to genomic instability in cells.
miR-203 and miR-320 Regulate Bone Morphogenetic Protein-2-Induced Osteoblast Differentiation by Targeting Distal-Less Homeobox 5 (Dlx5)
Authors: Laxman Navya , Mallmin Hans , Nilsson Olle , Kindmark Andreas
Published: December 2016

MicroRNAs (miRNAs) are a family of small, non-coding RNAs (17–24 nucleotides), which regulate gene expression either by the degradation of the target mRNAs or inhibiting the translation of genes. Recent studies have indicated that miRNA plays an important role in regulating osteoblast differentiation. In this study, we identified miR-203 and miR-320b as important miRNAs modulating osteoblast differentiation. We identified Dlx5 as potential common target by prediction algorithms and confirmed this by knock-down and over expression of the miRNAs and assessing Dlx5 at mRNA and protein levels and specificity was verified by luciferase reporter assays. We examined the effect of miR-203 and miR-320b on osteoblast differentiation by transfecting with pre- and anti-miRs. Over-expression of miR-203 and miR-320b inhibited osteoblast differentiation, whereas inhibition of miR-203 and miR-320b stimulated alkaline phosphatase activity and matrix mineralization. We show that miR-203 and miR-320b negatively regulate BMP-2-induced osteoblast differentiation by suppressing Dlx5, which in turn suppresses the downstream osteogenic master transcription factor Runx2 and Osx and together they suppress osteoblast differentiation. Taken together, we propose a role for miR-203 and miR-320b in modulating bone metabolism.
RNA Editing and Its Molecular Mechanism in Plant Organelles
Authors: Ichinose Mizuho , Sugita Mamoru
Published: December 2016

RNA editing by cytidine (C) to uridine (U) conversions is widespread in plant mitochondria and chloroplasts. In some plant taxa, “reverse” U-to-C editing also occurs. However, to date, no instance of RNA editing has yet been reported in green algae and the complex thalloid liverworts. RNA editing may have evolved in early land plants 450 million years ago. However, in some plant species, including the liverwort, Marchantia polymorpha, editing may have been lost during evolution. Most RNA editing events can restore the evolutionarily conserved amino acid residues in mRNAs or create translation start and stop codons. Therefore, RNA editing is an essential process to maintain genetic information at the RNA level. Individual RNA editing sites are recognized by plant-specific pentatricopeptide repeat (PPR) proteins that are encoded in the nuclear genome. These PPR proteins are characterized by repeat elements that bind specifically to RNA sequences upstream of target editing sites. In flowering plants, non-PPR proteins also participate in multiple RNA editing events as auxiliary factors. C-to-U editing can be explained by cytidine deamination. The proteins discovered to date are important factors for RNA editing but a bona fide RNA editing enzyme has yet to be identified.
Noncoding RNA Profiles in Tobacco- and Alcohol-Associated Diseases
Authors: Soares do Amaral Nayra , Cruz e Melo Natalia , De Melo Maia Beatriz , Malagoli Rocha Rafael
Published: December 2016

Tobacco and alcohol are the leading environmental risk factors in the development of human diseases, such as cancer, cardiovascular disease, and liver injury. Despite the copious amount of research on this topic, by 2030, 8.3 million deaths are projected to occur worldwide due to tobacco use. The expression of noncoding RNAs, primarily microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), is modulated by tobacco and alcohol consumption. Drinking alcohol and smoking cigarettes can modulate the expression of miRNAs and lncRNAs through various signaling pathways, such as apoptosis, angiogenesis, and inflammatory pathways—primarily interleukin 6 (IL-6)/signal transducer and activator of transcription 3 (STAT3), which seems to play a major role in the development of diseases associated with these risk factors. Since they may be predictive and prognostic biomarkers, they can be used both as predictors of the response to therapy and as a targeted therapy. Further, circulating miRNAs might be valuable noninvasive tools that can be used to examine diseases that are related to the use of tobacco and alcohol. This review discusses the function of noncoding RNAs in cancer and other human tobacco- and alcohol-associated diseases.