bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of rs405509 on APOEε4 Non-carriers in Non-demented Aging
Authors: Wu Dongpeng , Zhao Han , Gu Huali , Han Bin , Wang Qingqing , Man Xu , Zhao Renliang , Liu Xuejun , Sun Jinping
Published: June 2021

Background

There is evidence that the T allele of rs405509 located in the apolipoprotein E (APOE) promotor region is a risk factor for Alzheimer’s disease (AD). However, the effect of the T/T allele on brain function in non-demented aging is still unclear.

Methods

We analyzed the effects of the rs405509 T/T allele on cognitive performances using multiple neuropsychological tests and local brain function using resting-state functional magnetic resonance imaging (rs-fMRI).

Results

Significant differences were found between T/T carriers and G allele carriers on general cognitive status, memory, and attention (p < 0.05). Rs-fMRI analyses demonstrated decreased amplitude of low frequency fluctuation (ALFF) in the right middle frontal gyrus, decreased percent amplitude of fluctuation (PerAF) in the right middle frontal gyrus, increased regional homogeneity (ReHo) in the right cerebellar tonsil and decreased ReHo in the right putamen, and decreased degree centrality (DC) in the left middle frontal gyrus (p < 0.05, corrected). Furthermore, significant correlations were found between cognitive performance and these neuroimaging changes (p < 0.05).

Conclusion

These findings suggest that T/T allele may serve as an independent risk factor that can influence brain function in different regions in non-demented aging.
β-Sitosterol Ameliorates Endometrium Receptivity in PCOS-Like Mice: The Mediation of Gut Microbiota
Authors: Yu Yanyan , Cao Ying , Huang Wenling , Liu Yanxia , Lu Ying , Zhao Jiajing
Published: June 2021

Background: Polycystic ovary syndrome (PCOS), one of the most common endocrine diseases in women of childbearing age, has been found to be accompanied by changes in the gut microbiota. The Bu Shen Yang Xue formula (BSYXF) is a traditional Chinese medicine widely used for the treatment of PCOS. This study aimed to investigate whether the protective effects of β-sitosterol, the main active ingredient of BSYXF, on PCOS was mediated by regulating gut microbiota.

Methods: The presence of β-sitosterol in BSYXF was detected by liquid chromatography-mass spectrometry. The PCOS-like mouse model was induced by dehydroepiandrosterone. The fecal supernatant of β-sitosterol-treated mice was prepared for fecal microbiota transplantation (FMT). Body weight and wet weight of the uterus and ovary of the mice were recorded for organ index calculation. Hematoxylin and eosin stain was used to assess the endometrial morphology and microenvironment changes. Expression of endometrial receptivity markers cyclooxygenase-2 (COX-2), Integrin ανβ3, leukemia inhibitory factor (LIF), and homeobox A10 (HOXA10) in the endometrium were determined by immunohistochemistry and western blot analysis. Enzyme-linked immunosorbent assay was employed to detect the expression of follicle stimulating hormone (FSH), luteinizing hormone (LH), progesterone (P), and testosterone (T) in the serum. The diversity of gut microbiota was examined by 16S rDNA gene sequencing.

Results: With the treatment of β-sitosterol and β-sitosterol-FMT, the uterine index of PCOS-like mice increased, the ovarian index decreased, levels of COX-2, LH and T decreased, and levels of Integrin ανβ3, LIF, HOXA10, FSH, and P increased. Under β-sitosterol treatment, the structure of the gut microbiota in PCOS-like mice was also changed.

Conclusion: β-sitosterol regulates the endometrial receptivity of PCOS and harmonizes the sex hormone balance, which may be related to the changes in the structure and composition of gut microbiota, thus affecting the pathological process of PCOS.
Monitoring Carbon Ion Beams Transverse Position Detecting Charged Secondary Fragments: Results From Patient Treatment Performed at CNAO
Authors: Toppi Marco , Baroni Guido , Battistoni Giuseppe , Bisogni Maria Giuseppina , Cerello Piergiorgio , Ciocca Mario , De Maria Patrizia , De Simoni Micol , Donetti Marco , Dong Yunsheng , Embriaco Alessia , Ferrero Veronica , Fiorina Elisa , Fischetti Marta , Franciosini Gaia , Kraan Aafke Christine , Luongo Carmela , Malekzadeh Etesam , Magi Marco , Mancini-Terracciano Carlo , Marafini Michela , Mattei Ilaria , Mazzoni Enrico , Mirabelli Riccardo , Mirandola Alfredo , Morrocchi Matteo , Muraro Silvia , Patera Vincenzo , Pennazio Francesco , Schiavi Angelo , Sciubba Adalberto , Solfaroli-Camillocci Elena , Sportelli Giancarlo , Tampellini Sara , Traini Giacomo , Valle Serena Marta , Vischioni Barbara , Vitolo Viviana , Sarti Alessio
Published: June 2021

Particle therapy in which deep seated tumours are treated using 12C ions (Carbon Ions RadioTherapy or CIRT) exploits the high conformity in the dose release, the high relative biological effectiveness and low oxygen enhancement ratio of such projectiles. The advantages of CIRT are driving a rapid increase in the number of centres that are trying to implement such technique. To fully profit from the ballistic precision achievable in delivering the dose to the target volume an online range verification system would be needed, but currently missing. The 12C ions beams range could only be monitored by looking at the secondary radiation emitted by the primary beam interaction with the patient tissues and no technical solution capable of the needed precision has been adopted in the clinical centres yet. The detection of charged secondary fragments, mainly protons, emitted by the patient is a promising approach, and is currently being explored in clinical trials at CNAO. Charged particles are easy to detect and can be back-tracked to the emission point with high efficiency in an almost background-free environment. These fragments are the product of projectiles fragmentation, and are hence mainly produced along the beam path inside the patient. This experimental signature can be used to monitor the beam position in the plane orthogonal to its flight direction, providing an online feedback to the beam transverse position monitor chambers used in the clinical centres. This information could be used to cross-check, validate and calibrate, whenever needed, the information provided by the ion chambers already implemented in most clinical centres as beam control detectors. In this paper we study the feasibility of such strategy in the clinical routine, analysing the data collected during the clinical trial performed at the CNAO facility on patients treated using 12C ions and monitored using the Dose Profiler (DP) detector developed within the INSIDE project. On the basis of the data collected monitoring three patients, the technique potential and limitations will be discussed.
Preoperative Prediction of Metastasis for Ovarian Cancer Based on Computed Tomography Radiomics Features and Clinical Factors
Authors: Ai Yao , Zhang Jindi , Jin Juebin , Zhang Ji , Zhu Haiyan , Jin Xiance
Published: June 2021

Background

There is urgent need for an accurate preoperative prediction of metastatic status to optimize treatment for patients with ovarian cancer (OC). The feasibility of predicting the metastatic status based on radiomics features from preoperative computed tomography (CT) images alone or combined with clinical factors were investigated.

Methods

A total of 101 OC patients who underwent primary debulking surgery were enrolled. Radiomics features were extracted from the tumor volumes contoured on CT images with LIFEx package. Mann-Whitney U tests, least absolute shrinkage selection operator (LASSO), and Ridge Regression were applied to select features and to build prediction models. Univariate and regression analysis were applied to select clinical factors for metastatic prediction. The performance of models generated with radiomics features alone, clinical factors, and combined factors were evaluated and compared.

Results

Nine radiomics features were screened out from 184 extracted features to classify patients with and without metastasis. Age and cancer antigen 125 (CA125) were the two clinical factors that were associated with metastasis. The area under curves (AUCs) for the radiomics signature, clinical factors model, and combined model were 0.82 (95% CI, 0.66-0.98; sensitivity = 0.90, specificity = 0.70), 0.83 (95% CI, 0.67-0.95; sensitivity = 0.71, specificity = 0.8), and 0.86 (95% CI, 0.72-0.99, sensitivity = 0.81, specificity = 0.8), respectively.

Conclusions

Radiomics features alone or radiomics features combined with clinical factors are feasible and accurate enough to predict the metastatic status for OC patients.
Low Expression of ADCY4 Predicts Worse Survival of Lung Squamous Cell Carcinoma Based on Integrated Analysis and Immunohistochemical Verification
Authors: Liu Zhicong , Ru Lixin , Ma Zhenchao
Published: June 2021

Purpose

The molecular mechanism underlying the carcinogenesis and development of lung squamous cell carcinoma (LUSC) has not been sufficiently elucidated. This analysis was performed to find pivotal genes and explore their prognostic roles in LUSC.

Methods

A microarray dataset from GEO (GSE19188) and a TCGA-LUSC dataset were used to identify differentially co-expressed genes through Weighted Gene Co-expression Network Analysis (WGCNA) and differential gene expression analysis. We conducted functional enrichment analyses of differentially co-expressed genes and established a protein-protein interaction (PPI) network. Then, we identified the top 10 hub genes using the Maximal Clique Centrality (MCC) algorithm. We performed overall survival (OS) analysis of these hub genes among LUSC cases. GSEA analyses of survival-related hub genes were conducted. Ultimately, the GEO and The Human Protein Atlas (THPA) databases and immunohistochemistry (IHC) results from the real world were used to verify our findings.

Results

A list of 576 differentially co-expressed genes were selected. Functional enrichment analysis indicated that regulation of vasculature development, cell−cell junctions, actin binding and PPAR signaling pathways were mainly enriched. The top 10 hub genes were selected according to the ranking of MCC scores, and 5 genes were closely correlated with OS of LUSC. Additionally, GSEA analysis showed that spliceosome and cell adhesion molecules were associated with the expression of GNG11 and ADCY4, respectively. The GSE30219 and THPA databases and IHC results from the real world indicated that although GNG11 was not detected, ADCY4 was obviously downregulated in LUSC tissues at the mRNA and protein levels.

Conclusions

This analysis showed that survival-related hub genes are highly correlated to the tumorigenesis and development of LUSC. Additionally, ADCY4 is a candidate therapeutic and prognostic biomarker of LUSC.
Monoprophylaxis With Cephalosporins for Transrectal Prostate Biopsy After the Fluoroquinolone-Era: A Multi-Institutional Comparison of Severe Infectious Complications
Authors: Wenzel Mike , von Hardenberg Jost , Welte Maria N. , Doryumu Samuel , Hoeh Benedikt , Wittler Clarissa , Höfner Thomas , Kriegmair Maximilian C. , Michel Maurice S. , Chun Felix KH. , Herrmann Jonas , Mandel Philipp , Westhoff Niklas
Published: June 2021

Background

To compare severe infectious complication rates after transrectal prostate biopsies between cephalosporins and fluoroquinolones for antibiotic monoprophylaxis.

Material and Methods

In the multi-institutional cohort, between November 2014 and July 2020 patients received either cefotaxime (single dose intravenously), cefpodoxime (multiple doses orally) or fluoroquinolones (multiple-doses orally or single dose intravenously) for transrectal prostate biopsy prophylaxis. Data were prospectively acquired and retrospectively analyzed. Severe infectious complications were evaluated within 30 days after biopsy. Logistic regression models predicted biopsy-related infectious complications according to antibiotic prophylaxis, application type and patient- and procedure-related risk factors.

Results

Of 793 patients, 132 (16.6%) received a single dose of intravenous cefotaxime and were compared to 119 (15%) who received multiple doses of oral cefpodoxime and 542 (68.3%) who received fluoroquinolones as monoprophylaxis. The overall incidence of severe infectious complications was 1.0% (n=8). No significant differences were observed between the three compared groups (0.8% vs. 0.8% vs. 1.1%, p=0.9). The overall rate of urosepsis was 0.3% and did not significantly differ between the three compared groups as well.

Conclusion

Monoprophylaxis with third generation cephalosporins was efficient in preventing severe infectious complications after prostate biopsy. Single intravenous dose of cefotaxime and multiday regimen of oral cefpodoxime showed a low incidence of infectious complications <1%. No differences were observed in comparison to fluoroquinolones.
High LET-Like Radiation Tracks at the Distal Side of Accelerated Proton Bragg Peak
Authors: Horendeck Dakota , Walsh Kade D. , Hirakawa Hirokazu , Fujimori Akira , Kitamura Hisashi , Kato Takamitsu A.
Published: June 2021

Proton therapy is a type of hadron radiotherapy used for treating solid tumors. Unlike heavy charged elements, proton radiation is considered to be low LET (Linear Energy Transfer) radiation, like X-rays. However, the clinical SOBP (Spread Out Bragg Peak) proton radiation is considered to be higher in relative biological effectiveness (RBE) than both X-ray and their own entrance region. The RBE is estimated to be 1.1–1.2, which can be attributed to the higher LET at the SOBP region than at the entrance region. In order to clarify the nature of higher LET near the Bragg peak of proton radiation and its potential cytotoxic effects, we utilized a horizontal irradiation system with CHO cells. Additionally, we examined DNA repair mutants, analyzed cytotoxicity with colony formation, and assessed DNA damage and its repair with γ-H2AX foci assay in a high-resolution microscopic scale analysis along with the Bragg peak. Besides confirming that the most cytotoxic effects occurred at the Bragg peak, extended cytotoxicity was observed a few millimeters after the Bragg peak. γ-H2AX foci numbers reached a maximum at the Bragg peak and reduced dramatically after the Bragg peak. However, in the post-Bragg peak region, particle track-like structures were sporadically observed. This region contains foci that are more difficult to repair. The peak and post-Bragg peak regions contain rare high LET-like radiation tracks and can cause cellular lethality. This may have caused unwanted side effects and complexities of outputs for the proton therapy treatment.
Patterns of Safety Incidents in a Neonatal Intensive Care Unit
Authors: Brado Luise , Tippmann Susanne , Schreiner Daniel , Scherer Jonas , Plaschka Dorothea , Mildenberger Eva , Kidszun André
Published: June 2021

Introduction: Safety incidents preceding manifest adverse events are barely evaluated in neonatal intensive care units (NICUs). This study aimed at identifying frequency and patterns of safety incidents in our NICU.

Methods: A 6-month prospective clinical study was performed from May to October 2019 in a German 10-bed level III NICU. A voluntary, anonymous reporting system was introduced, and all neonatal team members were invited to complete paper-based questionnaires following each particular safety incident. Safety incidents were defined as safety-related events that were considered by the reporting team member as a “threat to the patient's well-being” which “should ideally not occur again.”

Results: In total, 198 safety incidents were analyzed. With 179 patients admitted, the incident/admission ratio was 1.11. Medication errors (n = 94, 47%) and equipment problems (n = 54, 27%) were most commonly reported. Diagnostic errors (n = 19, 10%), communication problems (n = 12, 6%), errors in documentation (n = 9, 5%) and hygiene problems (n = 10, 5%) were less frequent. Most safety incidents were noticed after 4–12 (n = 52, 26%) and 12–24 h (n = 47, 24%), respectively. Actual harm to the patient was reported in 17 cases (9%) but no life-threatening or serious events occurred. Of all safety incidents, 184 (93%) were considered to have been preventable or likely preventable. Suggestions for improvement were made in 132 cases (67%). Most often, implementation of computer-assisted tools and processes were proposed.

Conclusion: This study confirms the occurrence of various safety incidents in the NICU. To improve quality of care, a graduated approach tailored to the specific problems appears to be prudent.
Quality Improvement to Increase Breastfeeding in Preterm Infants: Systematic Review and Meta-Analysis
Authors: Fang Lingyu , Wu Lianqiang , Han Shuping , Chen Xiaohui , Yu Zhangbin
Published: June 2021

Background and Objective:Due to its numerous health benefits, breast milk (BM) is recommended for preterm infants. Despite such recommendations, the rates of breastfeeding in preterm infants are lower than that in term infants. Quality improvement (QI) bundles increase breastfeeding in preterm infants, but their replication in neonatal intensive care units has had inconsistent outcomes.

Methods:We used the Population or Problem, Intervention, Comparison, and Outcomes (PICO) framework to develop our search strategy, and searched MEDLINE, Embase, and the Cochrane Library from inception through January 15, 2021. Studies describing any active QI intervention to increase BM use in preterm infants were included. The primary outcome measure was the rate of any breastfeeding or exclusive mother's own milk (MOM) at discharge or during hospitalization.

Results:Sixteen studies were eligible for inclusion and showed an acceptable risk of bias, and included 1 interrupted time series, study 3 controlled before-and-after studies, and 12 uncontrolled before-and-after studies; of these, 3 studies were excluded due to insufficient dichotomous data, 13 were included in the meta-analysis. In the meta-analysis, the rate of any breastfeeding was significantly improved at discharge and during hospitalization after QI [risk ratio (RR) = 1.23, 95% confidence interval (CI): 1.14–1.32,P< 0.00001 andRR= 1.89, 95% CI: 1.09–3.29,P= 0.02, respectively]. The rate of exclusive MOM after QI was also significantly increased at discharge (RR= 1.51, 95% CI: 1.04–2.18,P= 0.03), but not during hospitalization (RR= 1.53, 95% CI: 0.78–2.98,P= 0.22). However, after sensitivity analysis, the comprehensive results still suggested that QI could significantly improve the rate of exclusive MOM during hospitalization (RR= 1.21, 95% CI: 1.08–1.35,P= 0.001). Funnel plots and Egger's test indicated publication bias in the rate of any BF at discharge. We corrected publication bias by trim and fill analysis, and correctedRRto 1.272, 95% CI: (1.175, 1.369), which was consistent with the results of the initial model.

Conclusions:A QI bundle appears to be effective for promoting BM use in preterm infants at discharge or during hospitalization.
Relationship Between Histological Features of Non-alcoholic Fatty Liver Disease and Ectopic Fat on Magnetic Resonance Imaging in Children and Adolescents
Authors: Lee Eun Hye , Kim Ji Young , Yang Hye Ran
Published: June 2021

Objectives: To investigate the association between ectopic fat content in the liver and pancreas, obesity-related metabolic components, and histological findings of non-alcoholic fatty liver disease (NAFLD) in children.

Methods: This cross-sectional study investigated 63 children with biopsy-proven NAFLD who underwent magnetic resonance imaging (MRI), anthropometry, laboratory tests, and body composition analysis. Clinical and metabolic parameters, MRI-measured hepatic fat fraction (HFF) and pancreatic fat fraction (PFF), and histological findings were analyzed.

Results: In a total of 63 children (48 boys, median age 12.6 years, median body mass index z-score 2.54), HFF was associated with histological steatosis [10.4, 23.7, and 31.1% in each steatosis grade, P < 0.001; Spearman's rho coefficient (rs) = 0.676; P < 0.001] and NAFLD activity score (rs = 0.470, P < 0.001), but not with lobular inflammation, hepatocyte ballooning, and hepatic fibrosis. PFF was not associated with any histological features of the liver. Waist circumference-to-height ratio and body fat percentage were associated with the steatosis grade (P = 0.006 and P = 0.004, respectively). Alanine aminotransferase was not associated with steatosis but was associated with lobular inflammation (P = 0.008). Lobular inflammation was also associated with high total cholesterol and low-density lipoprotein cholesterol and metabolic syndrome (P = 0.015, P = 0.036, and P = 0.038, respectively).

Conclusions: Hepatic steatosis on MRI was only associated with the histological steatosis grade, while elevated serum levels of liver enzymes and lipids were related to the severity of lobular inflammation. Therefore, MRI should be interpreted in conjunction with the anthropometric and laboratory findings in pediatric patients.
BPC 157 as a Therapy for Retinal Ischemia Induced by Retrobulbar Application of L-NAME in Rats
Authors: Zlatar Mirna , Kokot Antonio , Vuletic Lovorka Batelja , Masnec Sanja , Kralj Tamara , Perisa Marija Milkovic , Barisic Ivan , Radic Bozo , Milanovic Kristina , Drmic Domagoj , Seiwerth Sven , Sikiric Predrag
Published: June 2021

Providing NO-system importance, we suggest that one single application of the NOS-blocker L-NAME may induce retinal ischemia in rats, and that the stable pentadecapeptide BPC 157 may be the therapy, since it may interact with the NO-system and may counteract various adverse effects of L-NAME application. A rat retinal ischemia study was conducted throughout 4 weeks, including fundoscopy, behavior presentation, tonometry, and histology assessment. Retrobulbar L-NAME application (5 mg/kg; 0.5 mg/0.1 ml saline/each eye) in rats immediately produced moderate generalized irregularity in the diameter of blood vessels with moderate atrophy of the optic disc and faint presentation of the choroidal blood vessels, and these lesions rapidly progressed to the severe stage. The specific L-NAME–induced vascular failure points to normal intraocular pressure (except to very transitory increase upon drug retrobulbar administration). When BPC 157 (10 μg; 10 ng/kg, as retrobulbar application, 1 μg; 1 ng/0.1 ml saline/each eye) is given at either 20 min after L-NAME or, lately, at 48 h after L-NAME, the regular retrobulbar L-NAME injection findings disappear. Instead, fundoscopy demonstrated only discrete generalized vessel caliber irregularity with mild atrophy of the optic disc, and then, quite rapidly, normal eye background and choroidal blood vessels, which remain in all of the subsequent periods. Also, histology assessment at 1, 2, and 4 weeks shows that BPC 157 counteracted the damaged inner plexiform layer and inner nuclear layer, and revealed normal retinal thickness. The poor behavioral presentation was also rescued. Thus, while further studies will be done, BPC 157 counteracted L-NAME–induced rat retinal ischemia.
Post-Translational Modifications of Deubiquitinating Enzymes: Expanding the Ubiquitin Code
Authors: Wang Yanfeng , Wang Feng
Published: June 2021

Post-translational modifications such as ubiquitination play important regulatory roles in several biological processes in eukaryotes. This process could be reversed by deubiquitinating enzymes (DUBs), which remove conjugated ubiquitin molecules from target substrates. Owing to their role as essential enzymes in regulating all ubiquitin-related processes, the abundance, localization, and catalytic activity of DUBs are tightly regulated. Dysregulation of DUBs can cause dramatic physiological consequences and a variety of disorders such as cancer, and neurodegenerative and inflammatory diseases. Multiple factors, such as transcription and translation of associated genes, and the presence of accessory domains, binding proteins, and inhibitors have been implicated in several aspects of DUB regulation. Beyond this level of regulation, emerging studies show that the function of DUBs can be regulated by a variety of post-translational modifications, which significantly affect the abundance, localization, and catalytic activity of DUBs. The most extensively studied post-translational modification of DUBs is phosphorylation. Besides phosphorylation, ubiquitination, SUMOylation, acetylation, oxidation, and hydroxylation are also reported in DUBs. In this review, we summarize the current knowledge on the regulatory effects of post-translational modifications of DUBs.
Efficacy and Safety of Tripterygium Wilfordii Hook. F for Connective Tissue Disease-Associated Interstitial Lung Disease:A Systematic Review and Meta-Analysis
Authors: Li Yehui , Zhu Wen , He Hailang , Garov Yordan Angelov , Bai Le , Zhang Li , Wang Jing , Wang Jinghai , Zhou Xianmei
Published: June 2021

Background: Tripterygium wilfordii Hook. F (TwHF), a Chinese herbal medicine used to treat CTD-ILD patients in China, has been previously found to have immunoinhibitory, antifibrotic and anti inflammatory effects. It has also shown good results in treating autoimmune and inflammatory diseases.

Objectives: This systematic review and meta-analysis aims to evaluate the efficacy and safety of TwHF for CTD-ILD.

Methods: A systematic search was performed on PubMed, Embase, Cochrane Library, Web of Science, PsycINFO, Scopus, CNKI, Wanfang, VIP, and CBM databases up to May 2021. Randomized controlled trials (RCTs) comparing TwHF plus conventional therapy versus conventional therapy alone were included. We followed the PRISMA checklist, and applied Cochrane handbook 5.1.0 and RevMan 5.3 for data analysis and quality evaluation of the included studies.

Results: Based on Cochrane handbook 5.1.0, nine RCTs consisting 650 patients met the inclusion/exclusion criteria and were selected for further analysis. The obtained data showed significant improvement in lung function with TwHF plus conventional treatment compared with conventional treatment (post-treatment FVC% (MD= 8.68, 95%Cl (5.10, 12.26), p < 0.00001), FEV1% (MD = 11.24, 95%Cl (6.87, 15.61), p < 0.00001), TLC% (MD = 5.28, 95%Cl (0.69, 9.87), p = 0.02)], but no significant difference in the post-treatment DLCO% [(MD = 4.40, 95%Cl (−2.29, 11.09), p = 0.20)]. Moreover, the data showed that TwHF combined with conventional treatment significantly reduced the HRCT integral of patients [MD = -0.65, 95% (-1.01, -0.30), p = 0.0003], the level of erythrocyte sedimentation rate (MD = −9.52, 95%Cl (−11.55, −7.49), p < 0.00001), c-reactive protein (CRP) (MD = −8.42, 95%Cl (−12.47, −4.38), p < 0.0001), and rheumatoid factor (MD = −25.48, 95%Cl (−29.36, −21.60), p < 0.00001). Compared to conventional therapy, TwHF combined with conventional therapy significantly improved clinical effects (RR = 1.33, 95%Cl (1.17, 1.51), p < 0.0001), in five trials with 354 patients. In terms of improvement of symptoms and signs, the TwHF group showed a more significant improvement than the conventional treatment group (Cough (MD = −0.96, 95%Cl (−1.43, −0.50), p < 0.0001), velcro rales (MD = −0.32, 95%Cl (−0.44, −0.20), p < 0.00001), shortness of breath (MD = −1.11, 95%Cl (−1.67, −0.56), p < 0.0001)], but no statistical difference in dyspnea (MD = −0.66, 95%Cl (−1.35, 0.03), p = 0.06). There was no statistical significance in the incidence of adverse reactions.

Conclusion: The performed meta-analysis indicated that TwHF combined with conventional treatment was more beneficial to patients for improving symptoms, lung function and laboratory indicators. As it included studies with relatively small sample size, the findings require confirmation by further rigorously well-designed RCTs.
Leaf Counting: Fusing Network Components for Improved Accuracy
Authors: Farjon Guy , Itzhaky Yotam , Khoroshevsky Faina , Bar-Hillel Aharon
Published: June 2021

Leaf counting in potted plants is an important building block for estimating their health status and growth rate and has obtained increasing attention from the visual phenotyping community in recent years. Two novel deep learning approaches for visual leaf counting tasks are proposed, evaluated, and compared in this study. The first method performs counting via direct regression but using multiple image representation resolutions to attend leaves of multiple scales. The leaf count from multiple resolutions is fused using a novel technique to get the final count. The second method is detection with a regression model that counts the leaves after locating leaf center points and aggregating them. The algorithms are evaluated on the Leaf Counting Challenge (LCC) dataset of the Computer Vision Problems in Plant Phenotyping (CVPPP) conference 2017, and a new larger dataset of banana leaves. Experimental results show that both methods outperform previous CVPPP LCC challenge winners, based on the challenge evaluation metrics, and place this study as the state of the art in leaf counting. The detection with regression method is found to be preferable for larger datasets when the center-dot annotation is available, and it also enables leaf center localization with a 0.94 average precision. When such annotations are not available, the multiple scale regression model is a good option.
Latitudinal and Longitudinal Trends of Seed Traits Indicate Adaptive Strategies of an Invasive Plant
Authors: Zhou Lifeng , Yu Hongwei , Yang Kaiwen , Chen Li , Yin Wandong , Ding Jianqing
Published: June 2021

Invasive plants may change their seed traits to adapt to the environment and facilitate their performance. Studies on variation in seed traits among populations of an invader along latitudes/longitudes may assist in revealing how invasive plants cope with variable climates. In this study, we collected seeds of 26 populations of the global invasive plant Ambrosia artemisiifolia along ranges spanning 23° latitudes and 20° longitudes that are highly correlated in its invasive range in China. We measured over 20 seed traits, including seed morphology, phytohormone, nutrients, and germination, and investigated how the climate along the latitudes affects those traits. We found that germination time was significantly delayed with increasing latitude and longitude, while the reversed patterns were true for the germination rate. From low to high latitude, seed size, abscisic acid, and fatty acid were increased, likely affecting seed germination. Our analysis further demonstrated that temperature is the dominant driver of the variability in seed traits and germination. Germination rates of larger seeds in cold ranges were lower, while smaller seeds from warm ranges germinated faster, likely indicating adaptive strategies of the invasive plant in seed trait functional ecology. Together, our findings provide new insights into understanding the seed adaptation strategies during the invasion process and the underlying physiological and biochemical mechanisms involved.
Can Team Resilience Boost Team Creativity Among Undergraduate Students? A Sequential Mediation Model of Team Creative Efficacy and Team Trust
Authors: Fan Mudan , Cai Wenjing , Jiang Lin
Published: June 2021

Although recent literature has highlighted the critical role of resilience in creativity literature, existing findings have failed to indicate the processes through which resilience contributes to creativity at the graduate level. The current study fills this gap by hypothesizing the influence of team resilience on team creativity through a sequential mediating mechanism. A time lagged research study was conducted, and a sample of 201 undergraduate students and their teacher filled out questionnaires at three different time points (with 2-week intervals). After aggregating the data at the team level, we employed the PROCESS macro in SPSS to analyze data and test all the hypotheses through performing a sequential mediation analysis. We found that (a) team resilience would predict team creativity; and (b) team efficacy and team trust sequentially mediated the relation between team resilience and team creativity. The results in our study advance the emergent literature on linking resilience and creativity for the practical applications of resilience and creativity in education settings.
What Intervention Techniques Are Effective in Changing Positive Affective Variables and Physical Activity? A Systematic Review and Meta-Analysis
Authors: Chen Cheng , Finne Emily , Kopp Alexandra , Jekauc Darko
Published: June 2021

A recent meta-analysis has demonstrated that positive affective variables (PAVs) partially mediate physical activity (PA) interventions. However, the effectiveness of each intervention technique on PAVs and PA is still unknown. Thus, this meta-analytic review included two primary objectives: (1) to summarize intervention effects on PA and PAVs; (2) to examine each behavior change technique's effectiveness in modifying PAVs and PA. Following PRISMA protocols, we had searched five electronic databases by April 1, 2020. The random-effect model in the Comprehensive Meta-Analysis Version 3 was adopted to perform these meta-analytic analyses. The search identified 1,742 articles, and 37 studies (49 datasets) met our inclusion criteria. Finally, inferential statistics yielded that: the utilization of “teach to use prompts/cues,” “facilitate social comparison,” and “provide information on consequences of behavior in general” had positive effects on PA or PAVs outcomes; the utilization of “barrier identification/problem solving” and “plan social support/social change” negatively affected on PA or PAVs outcomes. However, there was considerable heterogeneity in the findings. Nonetheless, this paper has considerable implications for guiding future comparative intervention studies to achieve more reliable outcomes.
Misunderstandings in ART Triadic Interactions: A Qualitative Comparison of First and Follow-Up Visits
Authors: Rossi Maria Grazia , Vegni Elena , Menichetti Julia
Published: June 2021

Background

Misunderstandings in medical interactions can compromise the quality of communication and affect self-management, especially in complex interactions like those in the assisted reproductive technology (ART) field. This study aimed to detect and describe misunderstandings in ART triadic visits. We compared first and follow-up visits for frequency, type, speakers, and topics leading to misunderstandings.

Methods

We purposively sampled 20 triadic interactions from a corpus of 85 visits. We used a previously developed coding scheme to detect different types of misunderstandings (i.e., with strong, acceptable, and weak evidence). We analyzed also the different topics leading to strong misunderstandings (direct expressions of lack of understanding, pragmatic alternative understandings, semantic alternative understandings) to provide insights about the contents of the consultation that may need particular attention and care.

Findings

We detected an overall number of 1078 misunderstandings in the 20 selected visits. First visits contained almost two-third of the misunderstandings (n = 680, 63%). First visits were particularly rich in misunderstandings with acceptable evidence (e.g., clarifications and checks for understanding), compared to follow-up visits. In first visits, doctors’ turns more frequently than couples’ turns contained misunderstandings, while in follow-up visits it was the other way around. Looking at the couple, the majority of the misunderstandings were expressed by the woman (n = 241, 22%) rather than by the man (n = 194, 18%). However, when weighting for their number of turns, 9% of the men’s turns included an expression of misunderstanding, compared to the 7% of the women’s turns. Finally, more than half of the misunderstandings with strong evidence were about history-taking and treatment-related topics, and while the history-taking ones were particularly frequent in first visits the treatment-related ones were more present in follow-up visits.

Discussion

Findings indicate that first visits may deserve particular attention to avoid misunderstandings, as they are the moment where a shared understanding can be harder to reach. In particular, misunderstandings happening in first visits seem mostly related to physicians having to reconstruct the clinical history of patients, while those in the follow-up visits seem to reflect residual and unsolved doubts from the couple, especially concerning treatments.
Association Between Mental Imagery and Change of Direction Performance in Young Elite Soccer Players of Different Maturity Status
Authors: Sariati Dorsaf , Zouhal Hassane , Hammami Raouf , Clark Cain C. T. , Nebigh Ammar , Chtara Mokhtar , Hackney Anthony C. , Souissi Nizar , Granacher Urs , Ben Ounis Omar
Published: June 2021

Previous studies have not considered the potential influence of maturity status on the relationship between mental imagery and change of direction (CoD) speed in youth soccer. Accordingly, this cross-sectional study examined the association between mental imagery and CoD performance in young elite soccer players of different maturity status. Forty young male soccer players, aged 10-17 years, were assigned into two groups according to their predicted age at peak height velocity (PHV) (Pre-PHV; n = 20 and Post-PHV; n = 20). Participants were evaluated on soccer-specific tests of CoD with (CoDBall-15m) and without (CoD-15m) the ball. Participants completed the movement imagery questionnaire (MIQ) with the three- dimensional structure, internal visual imagery (IVI), external visual imagery (EVI), as well as kinesthetic imagery (KI). The Post-PHV players achieved significantly better results than Pre-PHV in EVI (ES = 1.58, large; p < 0.001), CoD-15m (ES = 2.09, very large; p < 0.001) and CoDBall-15m (ES = 1.60, large; p < 0.001). Correlations were significantly different between maturity groups, where, for the pre-PHV group, a negative very large correlation was observed between CoDBall-15m and KI (r = –0.73, p = 0.001). For the post-PHV group, large negative correlations were observed between CoD-15m and IVI (r = –0.55, p = 0.011), EVI (r = –062, p = 0.003), and KI (r = –0.52, p = 0.020). A large negative correlation of CoDBall-15m with EVI (r = –0.55, p = 0.012) and very large correlation with KI (r = –0.79, p = 0.001) were also observed. This study provides evidence of the theoretical and practical use for the CoD tasks stimulus with imagery. We recommend that sport psychology specialists, coaches, and athletes integrated imagery for CoD tasks in pre-pubertal soccer players to further improve CoD related performance.
Psychological Flexibility and Self-Compassion as Predictors of Well-Being: Mediating Role of a Balanced Time Perspective
Authors: Pyszkowska Anna , Rönnlund Michael
Published: June 2021

Measures of psychological flexibility and self-compassion are strongly associated with well-being. The aim of the present study was to test the hypothesis that these relationships are mediated by a balanced time perspective, a proposed ideal way of relating to the past, present, and future that may correspond with an ability to flexibly switch temporal focus. For this purpose, a Polish community sample (N = 421) responded to a web-survey including measures of psychological flexibility (AAQ-II), self-compassion (SCS), two measures of positive aspects of well-being (Satisfaction with Life, Quality of Life), and the Zimbardo Time Perspective Inventory (ZTPI). Structural equation models, involving a measure of deviation from a balanced time perspective (DBTP) as a mediator of relationships between latent-level psychological flexibility, self-compassion and well-being factors, were tested. We examined separate models for psychological flexibility and self-compassion and a model including both constructs. The results for separate models were consistent with partial mediation of relationships with well-being, both for psychological flexibility and self-compassion. Results for the analysis involving both constructs, suggested unique contributions of both to DBTP, which in turn predicted well-being, but the link between psychological flexibility and DBTP appeared to be the strongest. In further analyses, three ZTPI dimensions were identified as most critical, namely Past Positive, Present Fatalistic, and Past Negative, each of which were part of an indirect effect on well-being. Psychological flexibility in particular, showed a strong negative association with a Past Negative orientation. Taken together, the results indicate that time perspective is a factor to understand the links between psychological flexibility/self-compassion and well-being. While the results pertaining to self-compassion were consistent with results of a couple of prior studies, this is, to our knowledge, the first demonstration of a link between psychological flexibility and a balanced time perspective. These findings should be relevant for clinical research and practice.
Did You Enjoy It? The Role of Intensity-Trait Preference/Tolerance in Basic Psychological Needs and Exercise Enjoyment
Authors: Teixeira Diogo S. , Rodrigues Filipe , Machado Sérgio , Cid Luis , Monteiro Diogo
Published: June 2021

Promoting exercise regimens that aim at enhancing the quality of individuals’ subjective exercise experience can be challenging. Given the recent theoretical contributions regarding the possible interaction of exercise intensity-traits and several motivational variables, as well as their potential value for exercise adherence, the objective of this study was to examine the mediation role of basic psychological needs in the relationship between preference for and tolerance of exercise intensity and enjoyment. This cross-sectional study comprised a total of 160 exercisers (Mage = 34.12, SD = 9.23, 73 males) enrolled in several health clubs. All analyses were performed using SPSS v. 23.0/PROCESS v. 3.4. The results indicate that intensity-traits presented positive associations with enjoyment, and negative associations with all of needs frustration variables. A mediation role of needs frustration emerged in the intensity-traits and enjoyment associations that was analyzed according to relatable theoretical considerations.
Integration of the Confucian Culture on Cross-Cultural Conflict Management: The Role of the COVID-19 Pandemic
Authors: Liu Xiaoqin , Zhu Wenzhong , Liang Yanmei
Published: June 2021

Cross-culture conflict management is the major challenge for the Chinese enterprises going global along the Belt and Road Initiative. This study explores the feasibility of integrating the Confucian culture into cross-culture conflict management, and a special role is given to the COVID-19 pandemic. We combine the Confucian culture values and Hofstede’s cultural dimension theory and adopt the questionnaire survey methods on the Chinese multinational enterprises’ employees. The Cronbach’s Alpha method is also deployed to test the reliability and validity of the data. We find the significant integration of the Confucian culture into cross-culture conflict management. Furthermore, 16 sub-values of the Confucian culture are suggested to mitigate the cross-culture conflicts in multinational enterprises effectively. The findings imply that Chinese enterprises should consider new strategies to manage the cross-culture conflicts, especially during the COVID-19 pandemic.
Chinese College Students' Stress and Anxiety Levels Under COVID-19
Authors: Zhan Huali , Zheng Chunmei , Zhang Xianqin , Yang Meng , Zhang Lin , Jia Xu
Published: June 2021

The outbreak of COVID-19 at the end of 2019 has had a significant impact on people. While attention is paid to the immense physical harm it has caused, the psychological impact should not be underestimated. The main purpose of this study was to explore the stress, anxiety, and depression levels of different groups of college students during the COVID-19 pandemic. We conducted an online questionnaire survey of college students by using the Perceived Stress Scale (PSS-10), the Patient Health Questionnaire-9 items (PHQ-9), and the Self-rating Anxiety Scale (SAS). A total of 1,586 questionnaires were collected and analyzed in R language. The results showed that students with moderate to severe stress (PSS-10 ≥ 14) accounted for 67.50%; the detection rate of depression (PHQ-9 ≥ 5) reached 43.77%; and 20.60% of students had anxiety (SAS standard score ≥ 50). There were significant differences in PSS-10/SAS among different genders, majors, whether returning to school or not, and those with different psychological experiences (negative or positive, P < 0.05). It is notable that the median of female, medical student, non-resumption of schooling, and negative experience was higher than that of positive experience (P < 0.05). The results of principal component analysis showed that there were significant differences in PHQ-9, PSS-10, and SAS between the resumption of schooling group and the non-resumption group. Therefore, it is inferred that the stress and anxiety level of college students during the COVID-19 pandemic is generally high, especially for those who have not yet resumed school. Long-term negative emotions can easily lead to serious mental diseases such as cognitive impairment. Education departments should attach great importance to the mental health of college students, and it is necessary to provide precise psychological interventions for groups experiencing greater pressure levels and marked anxiety and depression.
Effects of Tai Chi on the Executive Function and Physical Fitness of Female Methamphetamine Dependents: A Randomized Controlled Trial
Authors: Menglu Shen , Ruiwen Liu , Suyong Yang , Dong Zhu
Published: June 2021

Purpose: Exercise improves the health and mental status of drug dependents. The way by which Tai Chi (TC) as a special exercise treatment affects executive functions (EFs) of methamphetamine (MA) dependents is yet to be established. This study aimed to explore the effects of TC on the EFs and physical fitness of MA dependents.

Methods: A total of 76 female MA dependents were randomly assigned to the exercise and control groups. The exercise group underwent three 60-min sessions of TC training per week for 12 weeks. The control group was trained with conventional exercises including the 9th Guang Bo Ti Cao and square dance. Physical fitness and EF assessments that evaluated inhibitory control (IC, go/no-go task), working memory (3-back task) and cognitive flexibility (switching task) were performed at baseline and at 12 weeks. A repeated-measures ANOVA was applied to analyze the differences of group and time.

Results: The exercise group showed decreased response time (RT) with a significant main effect of time on the go/no-go task [F(1, 68) = 9.6, p < 0.05]. The interaction effect between time and group was significant on accuracy [F(1, 61) = 4.73, p < 0.05], and the main effect of time was significant on RT [F(1, 61) = 4.66, p < 0.05] in the 3-back task of the exercise group. Significant changes in BMI [F(1, 68) = 19.57, p < 0.05], vital capacity [F(1, 68) = 6.00, p < 0.05], and systolic blood pressure [F(1, 68) = 6.11, p < 0.05] were observed in the exercise group.

Conclusion: These findings showed that 3 months of TC training can improve the IC and maintain the working memory and cognitive flexibility of MA dependents. Other data implied that TC may improve the physical fitness of MA dependents.

Clinical Trial Registration:http://www.chictr.org.cn/, ChiCTR1900022091.
Case Report: De Clerambault's Syndrome in Dementia With Lewy Bodies
Authors: Suehiro Takashi , Satake Yuto , Hashimoto Mamoru , Ikeda Manabu
Published: June 2021

Background: Erotomania, also known as de Clerambault's syndrome, is characterized by the delusion that a person has fallen in love with the patient. It occasionally appears secondary to psychiatric disorders and organic brain diseases. However, there have been no reports on cases secondary to dementia with Lewy bodies (DLB).

Case Presentation: The patient was an 83-year-old woman who lived alone. Mild cognitive impairment appeared at the age of 82 years. Soon after, she had the delusional conviction that her family doctor was in love with her. Her symptoms, such as gradually progressive cognitive impairment, cognitive fluctuations, and parkinsonism, indicated DLB. She was treated with a small dose of antipsychotic agents.

Conclusions: This case report suggests the possibility of de Clerambault's syndrome during the early stages of DLB. Further investigations are required to clarify the mechanism and treatment of de Clerambault's syndrome in patients with DLB.