bluuebunny/crossref_metadata_2025 huggingface dataset:
The Process of Patchy Expansion for Bamboo (Phyllostachys edulis) at the Bamboo–Broadleaf Forest Interface: Spreading and Filling in Order
Authors: Zeng Xiaoxia , Luo Huitan , Lu Jian , Zhu Xianglong , He Yaoli , Gong Chao , Ren Zewen , Huang Dongmei , Song Qingni , Yang Qingpei
Published: February 2024

Bamboo (Phyllostachys edulis) expansion to native adjacent forests has become an increasingly serious problem; however, expansion patterns of bamboo are still lacking research, especially at a community scale. Quantitative research on bamboo expansion patterns plays a significant role in understanding the bamboo expansion process, as well as expansion prevention and control. We analyzed the change in expansion pattern, expansion index, and expansion rate of bamboo in the bamboo-broadleaf transition zone sample plots, specifically from 2017 to 2021 and from the bamboo forest (representing the late stage of bamboo expansion) to the bamboo expansion front (representing the early stage of bamboo expansion). We found that the expansion of bamboo is a patchy expansion, including inner filling patch, boundary expanding patch, transboundary leaping patch, expansion–infill mixed patch, and stationary patch. From the early stage of bamboo expansion (year 2017 and bamboo expansion front) to the late stage of bamboo expansion (year 2021 and bamboo forest), the type of bamboo expansion patches transitioned from boundary expanding patch to inner filling patch and boundary expansion–inner infilling mixed patch. Additionally, the expansion rate of bamboo showed a declining trend. From 2017 to 2021, the bamboo forest (position of 0–20 m) and expansion front (position of 60–80 m) declined by 0.53 m/2a and 0.47 m/2a, respectively. Our research reveals that bamboo expansion exhibits a patchy expanding process, characterized by a sequence of “first spreading outward and then filling inward”, whether viewed from the type of expansion pattern or the expansion rate. This process involves continuous plaque addition, expansion, merger, and filling to complete the expansion of a bamboo population. These findings provide valuable insights into the process of bamboo expansion and have important implications for the management and control of bamboo forests.
Genetic Transformation of Forest Trees and Its Research Advances in Stress Tolerance
Authors: Li Yi , Yuan Yanhui , Hu Zijian , Liu Siying , Zhang Xi
Published: February 2024

Forests represent a vital natural resource and play a crucial role in climate regulation and maintaining biodiversity. However, the growth and development of forest trees are increasingly challenged by rising environmental pressures, particularly detrimental abiotic stressors. To address these challenges, genetic transformation technologies have emerged as effective solutions. Despite various difficulties in genetic transformation for forest trees, including prolonged life cycles, genetic diversity, interspecies variations, and complex regeneration systems, significant research progress has been achieved in tree gene editing, transgenic technology, and methods for delivering exogenous molecules. These technologies have the potential to enhance tree quality, increase productivity, and improve resistance to abiotic stress. This review provides an overview of the main methods and transformation receptors in tree genetic transformation. Additionally, we summarize several novel techniques, such as nanoparticle-mediated gene transformation, advanced gene editing technology, various novel delivery carriers, and non-genetically modified protein function interference through peptide aptamer. Notably, we also place emphasis on several referable genes from forest trees and common crops, together with their potential function for improving abiotic stress responses. Through this research, we aspire to achieve sustainable utilization and conservation of tree resources, thereby providing substantial support for future livelihoods and economic development.
Mixed-Effects Height Prediction Model for Juniperus procera Trees from a Dry Afromontane Forest in Ethiopia
Authors: Teshome Mindaye , Braz Evaldo Muñoz , Torres Carlos Moreira Miquelino Eleto , Raptis Dimitrios Ioannis , de Mattos Patricia Povoa , Temesgen Hailemariam , Rubio-Camacho Ernesto Alonso , Sileshi Gudeta Woldesemayat
Published: February 2024

Tree height is a crucial variable in forestry science. In the current study, an accurate height prediction model for Juniperus procera Hochst. ex Endl. trees were developed, using a nonlinear mixed-effects modeling approach on 1215 observations from 101 randomly established plots in the Chilimo Dry Afromontane Forest in Ethiopia. After comparing 14 nonlinear models, the most appropriate base model was selected and expanded as a mixed-effects model, using the sample plot as a grouping factor, and adding stand-level variables to increase the model’s prediction ability. Using a completely independent dataset of observations, the best sampling alternative for calibration was determined using goodness-of-fit criteria. Our findings revealed that the Michaelis–Menten model outperformed the other models, while the expansion to the mixed-effects model significantly improved the height prediction. On the other hand, incorporating the quadratic mean diameter and the stem density slightly improved the model’s prediction ability. The fixed-effects of the selected model can also be used to predict the mean height of Juniperus procera trees as a marginal solution. The calibration response revealed that a systematic selection of the three largest-diameter trees at the plot level is the most effective for random effect estimation across new plots or stands.
Waterlogging Hardening Effect on Transplant Stress Tolerance in Pinus densiflora
Authors: Byeon Siyeon , Kim Seohyun , Hong Jeonghyun , Kim Tae Kyung , Huh Woojin , Kim Kunhyo , Lee Minsu , Lee Hojin , Kim Sukyung , Park Chanoh , Bhusal Narayan , Han Ah Reum , Chandrasekaran Umashankar , Kim Hyun Seok
Published: February 2024

Waterlogging induces oxidative damage by accumulation of reactive oxygen species due to stomatal closure. Plants alter their physiological and molecular mechanisms to reduce and adapt to oxidative stress. This mechanism of adaptation to stress, known as hardening, can support future stress tolerance. Pinus densiflora seedlings were grown under waterlogging treatment for three years and then transplanted to another site to identify the waterlogging hardening effect on transplanting. Transcriptome analysis was conducted before and after transplanting, and physiological factors were measured after transplanting. After transplanting, wounding stress is the main cause of transplant stress, and 13 genes related to phenylpropanoid were upregulated for the recovery of wounded roots in waterlogged hardened seedlings. The leaf starch and soluble sugar content of the waterlogged hardened seedlings were 50.3% and 40.5% lower due to the formation of cell walls. However, auxin-related genes were downregulated in waterlogging hardened seedlings, resulting in a lower tendency for height growth in hardened waterlogged seedlings. Waterlogging hardening mitigated transplant stress by wounding more than non-hardening, whereas waterlogging hardening may negatively affect seedling height. Our study provides evidence for the hardening effect of long-term waterlogging on transplanted P. densiflora seedlings.
Wind Effect on External Fire Spread through Openings under the Protection of Horizontal Projections or Vertical Spandrels—A Numerical Study
Authors: Tang Yining , Tian Zhaofeng , Chen Xiao , Suendermann Brigitta , Gamble Grant , Huang Zefeng
Published: February 2024

A numerical investigation has been conducted to analyse the effect of wind on the vertical spread of fire through a front opening in a building’s external walls. The study utilises a building geometry established from previous experimental work conducted by the National Research Council of Canada (NRCC). A horizontal projection or a vertical spandrel is introduced above the opening of the compartment of fire origin. The purpose of the projection or spandrel is to inhibit the vertical spread of the fire, following the relevant requirements in the Australian National Construction Code (NCC). A computational fluid dynamics (CFD) package for fire-driven fluid flow, namely the Fire Dynamics Simulator (FDS), is employed to simulate the fire behaviour. The FDS model is validated against the NRCC’s experimental results, and a good agreement is achieved. Winds from three horizontal directions (front wind is normal to the opening, side wind is parallel to the opening, and back wind is from behind the building) have been investigated, with speeds ranging up to 10 m/s for each wind direction. Front wind speeds below 1 m/s are found to slightly enhance the vertical spread of the fire, while speeds exceeding 1 m/s are inclined to promote horizontal spread. The impact of side wind on the vertical fire spread was also found to vary with wind speed. The increase in the speed of back wind influences flame buoyancy, resulting in an augmented vertical fire spread. Furthermore, the numerical results reveal that a vertical spandrel of 1100 mm height is less effective in preventing vertical fire spread through openings, compared to a 1100 mm deep horizontal projection. The study suggests that the fire safety design in reducing the hazard of vertical fire spread through openings in buildings’ external walls could be further improved if the effect of wind is considered.
Regional-Scale Assessment of Burn Scar Mapping in Southwestern Amazonia Using Burned Area Products and CBERS/WFI Data Cubes
Authors: Ferro Poliana Domingos , Mataveli Guilherme , Arcanjo Jeferson de Souza , Dutra Débora Joana , Medeiros Thaís Pereira de , Shimabukuro Yosio Edemir , Pessôa Ana Carolina Moreira , de Oliveira Gabriel , Anderson Liana Oighenstein
Published: February 2024

Fires are one of the main sources of disturbance in fire-sensitive ecosystems such as the Amazon. Any attempt to characterize their impacts and establish actions aimed at combating these events presupposes the correct identification of the affected areas. However, accurate mapping of burned areas in humid tropical forest regions remains a challenging task. In this paper, we evaluate the performance of four operational BA products (MCD64A1, Fire_cci, GABAM and MapBiomas Fogo) on a regional scale in the southwestern Amazon and propose a new approach to BA mapping using fraction images extracted from data cubes of the Brazilian orbital sensors CBERS-4/WFI and CBERS-4A/WFI. The methodology for detecting burned areas consisted of applying the Linear Spectral Mixture Model to the images from the CBERS-4/WFI and CBERS-4A/WFI data cubes to generate shadow fraction images, which were then segmented and classified using the ISOSEG non-supervised algorithm. Regression and similarity analyses based on regular grid cells were carried out to compare the BA mappings. The results showed large discrepancies between the mappings in terms of total area burned, land use and land cover affected (forest and non-forest) and spatial location of the burned area. The global products MCD64A1, GABAM and Fire_cci tended to underestimate the area burned in the region, with Fire_cci underestimating BA by 88%, while the regional product MapBiomas Fogo was the closest to the reference, underestimating by only 7%. The burned area estimated by the method proposed in this work (337.5 km2) was 12% higher than the reference and showed a small difference in relation to the MapBiomas Fogo product (18% more BA). These differences can be explained by the different datasets and methods used to detect burned areas. The adoption of global products in regional studies can be critical in underestimating the total area burned in sensitive regions. Our study highlights the need to develop approaches aimed at improving the accuracy of current global products, and the development of regional burned area products may be more suitable for this purpose. Our proposed approach based on WFI data cubes has shown high potential for generating more accurate regional burned area maps, which can refine BA estimates in the Amazon.
Modeling Fire Hazards Induced by Volcanic Eruptions: The Case of Stromboli (Italy)
Authors: Guardo Roberto , Bilotta Giuseppe , Ganci Gaetana , Zuccarello Francesco , Andronico Daniele , Cappello Annalisa
Published: February 2024

We hereby present VolcFire, a new cellular automaton model for fire propagation aimed at the creation of fire hazard maps for fires of volcanic origin. The new model relies on satellite-derived input data for the topography, land-use, fuel, and humidity information, and produces probabilistic maps of fire propagation simulating fire spread. The model contains several simplifications compared to the current state-of-the-art, limiting its usability to plan fire-fighting interventions during an event in favour of a reduced computational load. The accuracy and reliability of the model are also discussed by presenting its ability to reproduce two recent fires on Stromboli island, with good spatial fit (Brier score of 0.146±0.002 for the 3 July 2019 volcanic fire, and of 0.073±0.001 for the 25 May 2022 anthropogenic fire) and less than 1.5% variation across multiple simulations for the same event.
High-Hydrostatic-Pressure-Stabilized White Grape Pomace to Improve the Oxidative Stability of Dry-Cured Sausages (“Salchichón”)
Authors: Carrapiso Ana Isabel , Martín-Mateos María Jesús , D’Arrigo Matilde , Delgado-Adámez Jonathan , Saraiva Jorge Alexandre , Ramírez-Bernabé María Rosario
Published: February 2024

White grape pomace (winery by-product) stabilized by blanching and high hydrostatic pressure has recently been successful at delaying lipid oxidation in burgers. The aim of this study was to investigate whether it can also delay lipid oxidation in dry-cured sausages, and to compare its effect when added at 0.5 and 3% with those of synthetic additives (sodium nitrite and ascorbic acid) and no additives (Control) in lipid and protein oxidation, the instrumental color, the sensory characteristics, and the volatile compounds. The pomace (68.7 ± 7.4 mmol Trolox g−1) was as effective as the additives at preventing lipid oxidation, resulting in values 3.2–3.8 times lower than the Control sausages. However, the pomace was not effective at decreasing the microbial counts, improving the instrumental and sensory color and the volatile compound profile, and decreasing the off-odor and off-flavor developed in the Control sausages. The lack of a detrimental effect of the pomace at 0.5% on the volatile compounds and the sensory characteristics and its benefits to delay lipid oxidation suggest that it might be useful to improve the oxidative stability. Conversely, at 3%, with a detrimental effect on some sensory characteristics and no benefits over the lower dosage, is not advisable.
Assessment of Sourdough Fermentation Impact on the Antioxidant and Anti-Inflammatory Potential of Pearl Millet from Burkina Faso
Authors: Gabriele Morena , Cavallero Andrea , Tomassi Elena , Arouna Nafiou , Árvay Július , Longo Vincenzo , Pucci Laura
Published: February 2024

Millet, a gluten-free cereal, has received attention for its environmental friendliness and higher protein content than other grains. It represents a staple food in many African countries, where fermentation is traditionally used for preserving food products and preparing different cereal-based products. This study aimed to assess the impact of sourdough fermentation on bioactive compounds and antioxidant and anti-inflammatory properties of pearl millet from Burkina Faso. Phenolic compounds were investigated spectrophotometrically and by HPLC-DAD. The antioxidant activity of unfermented (MF) and fermented (FeMF) millet was evaluated in vitro by spectrophotometric and fluorometric assays and ex vivo on oxidized human erythrocytes for hemolysis inhibition. Finally, the potential anti-inflammatory effect of FeMF and MF was evaluated on human adenocarcinoma cell line (HT-29) exposed to TNF-α inflammatory stimulus. Results revealed significantly higher levels of polyphenols, flavonoids, and in vitro antioxidant activity following millet fermentation. Notable differences in phenolic composition between FeMF and MF are observed, with fermentation facilitating the release of bioactive compounds such as gallic acid, quercetin, and rutin. A dose-dependent protection against oxidative hemolysis was observed in both FeMF- and MF-pretreated erythrocytes. Similarly, pretreatment with FeMF significantly reduced the levels of inflammatory markers in TNF-α-treated cells, with effects comparable to those of MF. Fermentation with sourdough represents a simple and low-cost method to improve the bioactive compounds content and in vitro antioxidant activity of millet flour with promising nutraceutical potential.
Investigating the Respiratory and Energy Metabolism Mechanisms behind ε-Poly-L-lysine Chitosan Coating’s Improved Preservation Effectiveness on Tremella fuciformis
Authors: Sun Junzheng , Wei Yingying , Li Longxiang , Tang Baosha , Yang Yanrong , Xiao Zheng , Chen Junchen , Lai Pufu
Published: February 2024

Freshly harvested Tremella fuciformis contains high water content with an unprotected outer surface and exhibits high respiration rates, which renders it prone to moisture and nutrient loss, leading to decay during storage. Our research utilized ε-poly-L-lysine (ε-PL) and chitosan as a composite coating preservative on fresh T. fuciformis. The findings revealed that the ε-PL + chitosan composite coating preservative effectively delayed the development of diseases and reduced weight loss during storage compared to the control group. Furthermore, this treatment significantly decreased the respiration rate of T. fuciformis and the activity of respiratory metabolism-related enzymes, such as alternative oxidase (AOX), cytochrome c oxidase (CCO), succinic dehydrogenase (SDH), 6-phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase (6-PGDH and G-6-PDH). Additionally, the composite coating preservative also delayed the depletion of ATP and ADP and maintained higher levels of the energy charge while preserving low levels of AMP. It also sustained heightened activities of Mg2+-ATPase, Ca2+-ATPase, and H+-ATPase enzymes. These results demonstrate that utilizing the ε-PL + chitosan composite coating preservative can serve as a sufficiently safe and efficient method for prolonging the shelf life of post-harvest fresh T. fuciformis.
Exploring Therapeutic Potential of Pleurotus ostreatus and Agaricus bisporus Mushrooms against Hyperlipidemia and Oxidative Stress Using Animal Model
Authors: Iqbal Touseef , Sohaib Muhammad , Iqbal Sanaullah , Rehman Habib
Published: February 2024

The mushrooms oyster (Pleurotus ostreatus) and white button (Agaricus bisporus) contain bioactive compounds that have potential beneficial effects on hypercholesterolemia and cardiovascular diseases. In this study, hypolipidemic and antioxidative potential of these mushrooms’ extract were explored using hypercholesterolemic (HC) rats as animal model. For the study, 56 adult rats were divided into seven groups, i.e., G1 (negative control), G2 (positive control group), G3 (HC rats with statin drug orally), G4 and G5 (HC rats @ 100 and 200 mg/kg body weight (BW) dose of oyster mushroom extracts), and G6 and G7 (HC rats @ 100 and 200 mg/kg BW dose of white button mushroom extracts). The hypercholesterolemia was induced experimentally in fasted rats through a high-fat diet along with injection of triton WR-1339. After 48 h, the treatment groups were given extract for 28 days along with standard diet. At the trial termination, we analyzed the blood sugar levels, antioxidant parameters, lipid profile, and renal function, as well as conducting liver function tests of the rats. The results indicated that positive control group rats exhibited increased levels of total cholesterol (TC), triglycerides (TG), low-density level (LDL), and very-low-density level (vLDL) by 19%, 37%, 52%, and 32%, respectively, and 53% decrease in HDL, whereas treatment groups that received 200 mg oyster and white button mushroom extracts reported 15%, 34%, 22% reduction in TC, TG, vLDL, respectively, and 22% improvement in HDL level. The enzyme profiles of different groups showed non-significant differences, although both mushroom extracts provision reduced glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels. Overall, the results indicated that mushroom extracts were helpful in maintaining oxidative stress and have the potential to improve dyslipidemia in the tested rat animal model.
New Horizons in Probiotics: Unraveling the Potential of Edible Microbial Polysaccharides through In Vitro Digestion Models
Authors: Wang Yuying , Zhu Shengyong , Zhang Tiantian , Gao Minjie , Zhan Xiaobei
Published: February 2024

In vitro digestion models, as innovative assessment tools, possess advantages such as speed, high throughput, low cost, and high repeatability. They have been widely applied to the investigation of food digestion behavior and its potential impact on health. In recent years, research on edible polysaccharides in the field of intestinal health has been increasing. However, there is still a lack of systematic reviews on the application of microbial-derived edible polysaccharides in in vitro intestinal models. This review thoroughly discusses the limitations and challenges of static and dynamic in vitro digestion experiments, while providing an in-depth introduction to several typical in vitro digestion models. In light of this, we focus on the degradability of microbial polysaccharides and oligosaccharides, with a particular emphasis on edible microbial polysaccharides typically utilized in the food industry, such as xanthan gum and gellan gum, and their potential impacts on intestinal health. Through this review, a more comprehensive understanding of the latest developments in microbial polysaccharides, regarding probiotic delivery, immobilization, and probiotic potential, is expected, thus providing an expanded and deepened perspective for their application in functional foods.
A Critical Review of Risk Assessment Models for Listeria monocytogenes in Seafood
Authors: Gonzales-Barron Ursula , Cadavez Vasco , De Oliveira Mota Juliana , Guillier Laurent , Sanaa Moez
Published: February 2024

Invasive listeriosis, due to its severe nature in susceptible populations, has been the focus of many quantitative risk assessment (QRA) models aiming to provide a valuable guide in future risk management efforts. A review of the published QRA models of Listeria monocytogenes in seafood was performed, with the objective of appraising the effectiveness of the control strategies at different points along the food chain. It is worth noting, however, that the outcomes of a QRA model are context-specific, and influenced by the country and target population, the assumptions that are employed, and the model architecture itself. Studies containing QRA models were retrieved through a literature search using properly connected keywords on Scopus and PubMed®. All 13 QRA models that were recovered were of short scope, covering, at most, the period from the end of processing to consumption; the majority (85%) focused on smoked or gravad fish. Since the modelled pathways commenced with the packaged product, none of the QRA models addressed cross-contamination events. Many models agreed that keeping the product’s temperature at 4.0–4.5 °C leads to greater reductions in the final risk of listeriosis than reducing the shelf life by one week and that the effectiveness of both measures can be surpassed by reducing the initial occurrence of L. monocytogenes in the product (at the end of processing). It is, therefore, necessary that future QRA models for RTE seafood contain a processing module that can provide insight into intervention strategies that can retard L. monocytogenes’ growth, such as the use of bacteriocins, ad hoc starter cultures and/or organic acids, and other strategies seeking to reduce cross-contamination at the facilities, such as stringent controls for sanitation procedures. Since risk estimates were shown to be moderately driven by growth kinetic parameters, namely, the exponential growth rate, the minimum temperature for growth, and the maximum population density, further work is needed to reduce uncertainties.
Effects of Paraquat, Dextran Sulfate Sodium, and Irradiation on Behavioral and Cognitive Performance and the Gut Microbiome in A53T and A53T-L444P Mice
Authors: Chaklai Ariel , O’Neil Abigail , Goel Shrey , Margolies Nick , Krenik Destine , Perez Ruby , Kessler Kat , Staltontall Elizabeth , Yoon Hong Ki (Eric) , Pantoja Montzerrat , Stagaman Keaton , Kasschau Kristin , Unni Vivek , Duvoisin Robert , Sharpton Thomas , Raber Jacob
Published: February 2024

Heterozygous carriers of the glucocerebrosidase 1 (GBA) L444P Gaucher mutation have an increased risk of developing Parkinson’s disease (PD). The GBA mutations result in elevated alpha synuclein (aSyn) levels. Heterozygous mice carrying one allele with the L444P mutation knocked-into the mouse gene show increased aSyn levels and are more sensitive to motor deficits following exposure to the neurotoxin (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) MPTP than wild-type mice. Paraquat (PQ), a herbicide, increases PD risk in most studies. Its effects on the brain involve alterations in the gut microbiome. Exposure to dextran sulfate sodium (DSS), a mouse model of colitis, can be used to determine whether gut microbiome alterations are sufficient to induce PD-relevant phenotypes. We rederived the A53T-L444P and A53T mouse lines to assess whether PQ, PQ in combination with radiation exposure (IR), and DSS have differential effects in A53T and A53T-L444P mice and whether these effects are associated with alterations in the gut microbiome. PQ and PQ + IR have differential effects in A53T and A53T-L444P mice. In contrast, effects of DSS are only seen in A53T-L444P mice. Exposure and genotype modulate the relationship between the gut microbiome and behavioral performance. The gut microbiome may be an important mediator of how environmental exposures or genetic mutations yield behavioral and cognitive impacts.
Whole Genome Sequencing and Comparative Genomics of Six Staphylococcus schleiferi and Staphylococcus coagulans Isolates
Authors: Abouelkhair Mohamed A. , Kania Stephen A.
Published: February 2024

Staphylococcus schleiferi and Staphylococcus coagulans, closely related bacterial species within the Staphylococcus genus, present a challenge in classification and diagnosis due to their close genetic proximity and overlapping phenotypic features. Moreover, our understanding of the virulence mechanisms in staphylococcal species, beyond the extensively studied Staphylococcus aureus, remains limited, underscoring the importance of using comparative data to enhance our insights into virulence within these bacterial species. This study employed a comprehensive approach, utilizing comparative genomics, to identify genomic distinctions between S. schleiferi and S. coagulans, aiming to address the challenges in the accurate classification and diagnosis of these organisms and identify unique features. Whole genome sequencing was performed on six clinical isolates, and their genomes were compared to identify variations in gene content and virulence factors. De novo assembly and annotation revealed two samples as S. coagulans and four samples as S. schleiferi. Analysis of the core genomes revealed conserved regions crucial for defining species identity, while accessory genomic elements contained unique genes, possibly impacting the pathogenicity of the species.
Full-Length Transcriptome Sequencing and RNA-Seq Analysis Offer Insights into Terpenoid Biosynthesis in Blumea balsamifera (L.) DC.
Authors: Ju Zhigang , Liang Lin , Zheng Yaqiang , Shi Hongxi , Zhao Wenxuan , Sun Wei , Pang Yuxin
Published: February 2024

Blumea balsamifera (L.) DC., an important economic and medicinal herb, has a long history of being used as a traditional Chinese medicine. Its leaves have always been used as a raw material for the extraction of essential oils, comprising large amounts of terpenoids, which have good therapeutic effects on many diseases, such as eczema, bacterial infection, and hypertension. However, the genetic basis of terpenoid biosynthesis in this plant is virtually unknown on account of the lack of genomic data. Here, a combination of next-generation sequencing (NGS) and full-length transcriptome sequencing was applied to identify genes involved in terpenoid biosynthesis at five developmental stages. Then, the main components of essential oils in B. balsamifera were identified using GC–MS. Overall, 16 monoterpenoids and 20 sesquiterpenoids were identified and 333,860 CCS reads were generated, yielding 65,045 non-redundant transcripts. Among these highly accurate transcripts, 59,958 (92.18%) transcripts were successfully annotated using NR, eggNOG, Swissprot, KEGG, KOG, COG, Pfam, and GO databases. Finally, a total of 56 differently expressed genes (DEGs) involved in terpenoid biosynthesis were identified, including 38 terpenoid backbone genes and 18 TPSs, which provide a significant amount of genetic information for B. balsamifera. These results build a basis for resource protection, molecular breeding, and the metabolic engineering of this plant.
Prevalence of Endocrinopathies in a Cohort of Patients with Rett Syndrome: A Two-Center Observational Study
Authors: Pepe Giorgia , Coco Roberto , Corica Domenico , Di Rosa Gabriella , Bossowski Filip , Skorupska Magdalena , Aversa Tommaso , Stagi Stefano , Wasniewska Malgorzata
Published: February 2024

Systematic data on endocrinopathies in Rett syndrome (RTT) patients remain limited and inconclusive. The aim of this retrospective observational two-center study was to assess the prevalence of endocrinopathies in a pediatric population of RTT patients. A total of 51 Caucasian patients (47 girls, 4 boys) with a genetically confirmed diagnosis of RTT were enrolled (mean age 9.65 ± 5.9 years). The patients were referred from the Rett Center of two Italian Hospitals for endocrinological evaluation. All the study population underwent clinical and auxological assessments and hormonal workups. MeCP2 mutations were detected in 38 cases (74.5%), CDKL5 deletions in 11 (21.6%), and FOXG1 mutations in 2 (3.9%). Overall, 40 patients were treated with anti-seizure medications. The most frequent endocrinological finding was short stature (47%), followed by menstrual cycle abnormalities (46.2%), weight disorders (45.1%), low bone mineral density (19.6%), hyperprolactinemia (13.7%) and thyroid disorders (9.8%). In the entire study population, endocrinopathies were significantly more frequent in patients with MeCP2 mutations (p = 0.0005), and epilepsy was more frequent in CDKL5 deletions (p = 0.02). In conclusion, our data highlighted that endocrinopathies are not rare in RTT, especially in patients with MeCP2 deletions. Therefore, in the context of a multidisciplinary approach, endocrinological evaluation should be recommended for RTT patients.
Genome-Wide Identification and Transcriptome Analysis of the Hsp70 Gene Family in Monodonta labio Reveals Its Role in Response to Nanoplastics Stress
Authors: He Jingjing , Han Xiaolu , Sun Shaolei , Jin Shihuai , Liu Mengyuan , Han Zhiqiang
Published: February 2024

For marine invertebrates, the disruption of organismal physiology and behavior by nanoplastics (NPs) has been extensively reported. Heat shock proteins (Hsps) are important for redundant protein breakdown, environmental changes, and intracellular protein transport. An exhaustive identification of Hsp70 genes and an experiment where different concentrations of NPs were stressed were performed to study how Hsp70 genes respond to NPs stress in Monodonta labio. Our results identified 15 members of Hsp70 within the genome of M. labio and provided insights into their responses to different concentrations of acute NP stress. Phylogenetic analyses revealed extensive amplification of the Hsp70 genes from the Hsc70 subfamily, with gene duplication events. As a result of NP stress, five of fifteen genes showed significant upregulation or downregulation. Three Hsp70 genes were highly expressed at an NP concentration of 0.1 mg/L, and no genes were downregulated. At 10 mg/L, they showed significant upregulation of two genes and significant downregulation of two genes. At 1 mg/L treatment, three genes were significantly downregulated, and no genes were significantly upregulated. Moreover, a purifying selection was revealed using a selection test conducted on duplicate gene pairs, indicating functional redundancy. This work is the first thorough examination of the Hsp70s in Archaeogastropoda. The findings improve knowledge of Hsp70s in molluscan adaptation to NP stress and intertidal living and offer essential data for the biological study of M. labio.
Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
Authors: Gorczynski Reginald
Published: February 2024

The field of clinical oncology has been revolutionized over the past decade with the introduction of many new immunotherapies the existence of which have depended to a large extent on experimentation with both in vitro analysis and the use of various animal models, including gene-modified mice. The discussion below will review my own laboratory’s studies, along with those of others in the field, on cancer immunotherapy. Our own studies have predominantly dwelt on two models of malignancy, namely a solid tumor model (breast cancer) and lymphoma. The data from our own laboratory, and that of other scientists, highlights the novel information so obtained, and the evidence that application of such information has already had an impact on immunotherapy of human oncologic diseases
Lignin Biosynthesis and Its Diversified Roles in Disease Resistance
Authors: Ma Qing-Hu
Published: February 2024

Lignin is complex, three-dimensional biopolymer existing in plant cell wall. Lignin biosynthesis is increasingly highlighted because it is closely related to the wide applications in agriculture and industry productions, including in pulping process, forage digestibility, bio-fuel, and carbon sequestration. The functions of lignin in planta have also attracted more attentions recently, particularly in plant defense response against different pathogens. In this brief review, the progress in lignin biosynthesis is discussed, and the lignin’s roles in disease resistance are thoroughly elucidated. This issue will help in developing broad-spectrum resistant crops in agriculture.
The Influence of Genetic Polymorphic Variability of the Catechol-O-methyltransferase Gene in a Group of Patients with a Diagnosis of Behavioural Addiction, including Personality Traits
Authors: Recław Remigiusz , Chmielowiec Krzysztof , Suchanecka Aleksandra , Boroń Agnieszka , Chmielowiec Jolanta , Strońska-Pluta Aleksandra , Kowalski Michał Tomasz , Masiak Jolanta , Trybek Grzegorz , Grzywacz Anna
Published: February 2024

Gambling Disorder (GD) is characterised by a harmful, enduring, and recurrent involvement in betting-related behaviours. Therefore, GD shares similar biological mechanisms and symptoms to substance use disorders (SUD). Therefore, in this study, we chose the behavioural addictions group. During the examination and recruitment to the study, it turned out that all the people undergoing treatment for gambling addiction were also addicted to amphetamines, which is consistent with the biological mechanism related to cerebral neurotransmission. The aim of the study was to investigate the association of the COMT gene polymorphism with behavioral addiction. The study group consisted of 307 participants: 107 men with gambling disorder and amphetamine dependency (mean age = 27.51, SD = 5.25) and 200 non-addicted, nor dependent, free from neuro-psychiatric disorders control group men (mean age = 20.20, SD = 4.51). Both groups were subjected to psychometric evaluation using the State-Trait Anxiety Inventory and the NEO Five-Factor Personality Inventory. Genomic DNA was extracted from venous blood following standard protocols. Determination of the rs4680 polymorphism in the COMT gene was performed using the real-time PCR technique. Statistically significant differences in the frequency of rs4680 genotypes were found in the tested sample of subjects compared with the control group (p = 0.03543). Subjects with gambling disorder and amphetamine use disorder compared to the control group obtained higher scores in the assessment of the STAI trait scale (p = 0.0019), state scale (p < 0.0000), and NEO-FFI Neuroticism scale (p < 0.0000). Significantly lower results were obtained for the NEO-FFI Agreeability scale (p < 0.0000). Additionally, a significant statistical impact of gambling disorder and amphetamine use disorder, and the COMT rs4680 genotype was demonstrated for the score of the STAI trait (p = 0.0351) and state (p = 0.0343) and the NEO-FFI Conscientiousness scale (p = 0.0018). We conclude that COMT and its polymorphic variant influence the development of addiction. Still, considering its multifactorial and polygenic nature, it should be combined with other factors such as personality.
Preliminary Assessment of Geohazards’ Impacts on Geodiversity in the Kratovska Reka Catchment (North Macedonia)
Authors: Aleksova Bojana , Lukić Tin , Milevski Ivica , Puhar Dušan , Marković Slobodan B.
Published: February 2024

This comprehensive study investigates the intricate interplay between geodiversity, geohazards, and anthropogenic influences within the Kratovska Reka catchment—an area distinguished by its remarkable geosites. Kratovska Reka, spanning a length of 17.3 km, serves as the left tributary to Kriva Reka. The watershed of Kratovska Reka, covering an area of 68.5 km2, is situated on the northwestern inclines of the Osogovo Mts in North Macedonia. Despite harboring exceptional geodiversity, the area lacks protective measures for its myriad geosites. Evaluating susceptibility to geohazards, including excessive erosion, landslides, and flash floods, this research identifies heightened risk zones, particularly in the valley of Kratovska Reka and its tributaries. A multi-hazard model reveals that 56.07% of the basin is vulnerable to geohazards. The study correlates lithological composition, relief features, and morphometric characteristics with geohazards, emphasizing the significance of paleovolcanic relief in resisting excessive erosion. Human-induced factors, notably deforestation and inappropriate land use, amplify geohazards. This research underscores the urgent need for geosite protection and sustainable land management to mitigate geohazards’ impacts. Additionally, it explores the correlation between land use practices and geodiversity, emphasizing the importance of responsible land management in safeguarding the geological and geomorphological values of the researched area.
“To Live Is a Matter of Time”: Memory, Survival and Queer Refugeehood in Ocean Vuong’s On Earth We’re Briefly Gorgeous
Authors: Soler i Arjona Sara
Published: February 2024

In his novel On Earth We’re Briefly Gorgeous (2019), Ocean Vuong attempts to reweave the historical threads that have been brutally severed by American imperialism, forced migration and the imperatives of assimilation, as a practice of survival. Drawing on his own experience as a Vietnamese refugee, Vuong situates a Vietnamese American queer protagonist at the centre of his non-linear narrative, which excavates the boy’s family history to trace the multiple histories of displacement informing who he is today. The novel’s temporal disorientation becomes a formulation of queer temporality that activates a critical reorientation of how experiences of refuge are typically represented—a coming into consciousness known as “refugeetude”. Such a critical reorientation serves a dual purpose. Firstly, by foregrounding the protagonist’s—and his family’s—shattered recollections, Vuong challenges dominant accounts of the Vietnam War and recovers the voices of those that are effaced by Western representation, thus assembling a more inclusive “just memory” of the war. Secondly, the novel disrupts the teleological narrative of progressive assimilation that is prevalent in refugee discourse by revealing the enduring forms of violence that displaced subjects must still face in contemporary America. By queering the normative temporality of refugee experience, the novel demonstrates how the characters’ refugeehood is not finite but ongoing, necessitating a continuous search for healing and resilience.
Risk of Social Isolation as a Contributing Factor to Diet Quality in Community-Dwelling Older Persons Living in the Australian Capital Territory—A Pilot Study
Authors: Low Elizabeth , D’Cunha Nathan M. , Georgousopoulou Ekavi , Naumovski Nenad , Bacon Rachel , Isbel Stephen , Brocklehurst Megan , Reynolds Matthew , Ryan Daena , Kellett Jane
Published: February 2024

Objectives: Social isolation is recognised as a risk factor in the inflammatory process. This study explored the association between social isolation and the Dietary Inflammatory Index (DII) in community-dwelling older persons. Methods: This cross-sectional pilot study recruited 107 community-dwelling people aged over 55 years living in the Australian Capital Territory. Participants completed an extensive food frequency questionnaire and provided anthropometric and sociodemographic data. Social isolation was evaluated using the Lubben Social Network Scale (LSNS). Diet quality was assessed using DII. Results: Average age was 70.1 (±8.61) years and 62.8% were female. The average DII score was −1.10 (±1.21), indicating an anti-inflammatory diet. Higher LSNS was associated with lower DII (b (95% CI) = −0.041 (−0.066, −0.17); p < 0.01) and was positively influenced by the number of people in household (b (95% CI) = 5.731 (2.336, 9.127); p = 0.001). Conclusion: Increased risk of social isolation was associated with an increased tendency towards a more inflammatory diet. Reducing social isolation may decrease the inflammatory component of dietary intake for older persons living independently in the community.
Vision-Related Quality of Life in Patients with Systemic Lupus Erythematosus
Authors: Bartol-Puyal Francisco de Asís , Chacón González María , Arias-Peso Borja , García Navarro Damián , Méndez-Martínez Silvia , Ruiz del Tiempo María Pilar , Sáez Comet Luis , Pablo Júlvez Luis
Published: February 2024

Purpose: To assess vision-related quality of life (VRQoL) in patients with systemic lupus erythematosus (SLE) under treatment with hydroxychloroquine (HCQ), and to find the influencing factors. Methods: Cross-sectional study enrolling SLE patients for less than ten years (Group 1), SLE patients for more than ten years (Group 2), and healthy controls (Group 3). SLE patients should be under treatment with HCQ but without ophthalmological affection. Schirmer test, best-corrected visual acuity (BCVA), axial length (AL) with optical biometry, and swept-source optical coherence tomography–angiography (OCTA) Triton (Topcon) were performed. All participants fulfilled the Impact of Visual Impairment questionnaire, and SLE patients answered the Lupus Impact Tracker (LIT) questionnaire. Additional data were obtained from clinical records. Results: A totals of 41 eyes (41 patients), 31 eyes (31 patients) and 45 eyes (45 volunteers) were enrolled in the study groups. The mean ages were 41.09 ± 9.56, 45.06 ± 8.47 and 40.25 ± 10.83 years, respectively (p = 0.10). The LIT outcomes were 33.49 ± 20.74 and 35.98 ± 22.66 (p = 0.63), respectively. Group 3 referred to a better VRQoL than Group 2 in all categories and than Group 1 in some of them. A linear regression analysis showed that serum ferritin, SLE activity scales, body-mass index (BMI), age, and BCVA influenced VRQoL. The LIT questionnaire was correlated to two categories of the Impact of Visual Impairment questionnaire (IVI). Conclusions: Despite no ophthalmological affection, SLE patients refer to poorer VRQoL because of disease activity and a low health-related quality of life, which has a negative influence on VRQoL. This masks the effect of other ophthalmological conditions such as dry eyes. Other variables influencing VRQoL are age and BMI, and BCVA, to a lesser extent.
Exploring the Link between Social Support and Patient-Reported Outcomes in Chronic Obstructive Pulmonary Disease Patients: A Cross-Sectional Study in Primary Care
Authors: Bouloukaki Izolde , Christodoulakis Antonios , Margetaki Katerina , Aravantinou Karlatou Antonia , Tsiligianni Ioanna
Published: February 2024

We aimed to explore the link between social support and various patient-reported outcome measures (PROMs) in primary care patients with COPD. This was a cross-sectional study with 168 patients with COPD from six primary care centers in Crete, Greece. We collected data on sociodemographic characteristics, medical history, disease-specific quality of life, the COPD Assessment Test (CAT), fatigue, the Fatigue Severity Scale (FSS), phycological parameters, Patient Health Questionnaire-9, General Anxiety Disorder-7, sleep complaints, the Pittsburg Sleep Quality Index, the Athens Insomnia scale (AIS), and the Epworth Sleepiness Scale. Social support was measured using the Multidimensional Scale of Perceived Social Support (MSPSS). Out of 168 patients with COPD, 114 (68.9%) exhibited low levels of social support. Low social support (MSPSS total ≤ 5) was positively associated with COPD symptoms (CAT score ≥ 10) (OR = 3.97, 95%CI:1.86–8.44; p < 0.01), fatigue (FSS ≥ 36) (OR = 2.74, 95%CI:1.31–5.74; p = 0.01), and insomnia symptoms (AIS ≥ 6) (OR = 5.17 95%CI:2.23–12.01; p < 0.01), while the association with depressive symptoms (PHQ-9 ≥ 10) was marginally significant (OR = 3.1, 95%CI:0.93–10.36; p = 0.07). Our results suggest that lower levels of social support are positively associated with PROMs in patients with COPD. Therefore, our findings show an additional way to improve the overall health of patients with COPD in primary care by putting social support at the epicenter of actions.
Effects of Peroneus Brevis versus Peroneus Longus Muscle Training on Muscle Function in Chronic Ankle Instability: A Randomized Controlled Trial
Authors: Ko Dukhan , Choi Yongchul , Lee Kyujin
Published: February 2024

Chronic ankle instability (CAI) is a common injury that can occur in daily life or sporting events. Injuries to the anterior talofibular, posterior talofibular, and calcaneofibular ligaments are common, and the core of rehabilitation training involves strengthening the peroneus muscle. Many studies on rehabilitation training have focused on strengthening the peroneus brevis muscle, and few studies have focused on specific training to strengthen the peroneus longus muscle. Therefore, this study aims to investigate changes in the symptoms and functions of patients by applying training to strengthen the peroneus longus and peroneus brevis muscles. Home-based training and mobile monitoring were utilized for 12 weeks, divided into peroneus brevis training (PBT) and peroneus longus training (PLT), in 52 adult males with CAI. Participation was voluntary, with enrollment done through a bulletin board, and intervention training allocation was randomly assigned and conducted in a double-blind manner. This study was registered as a trial protocol (KCT 0008478). Foot and ankle outcome scores (FAOS), isokinetic ankle strength tests, and Y-balance tests were performed before and after the intervention. Both PLT and PBT significantly improved in FAOS, inversion, and eversion at angular velocities of 30°/s and 120°/s and in the anterior and posterolateral directions of the Y-balance test (p < 0.05). Interaction effects by time and group were not significant for the FAOS (p > 0.05). However, PLT improved eversion muscle strength and muscle power to a greater degree, compared with PBT, in the anterior and posterolateral directions of the Y-balance test (p < 0.05). In conclusion, both PLT and PBT were effective for CAI patients; in addition, PLT had greater potential for improving strength and balance.