bluuebunny/crossref_metadata_2025 huggingface dataset:
Avian iPSC Derivation to Recover Threatened Wild Species: A Comprehensive Review in Light of Well-Established Protocols
Authors: Nogueira Iara Pastor Martins , Costa Guilherme Mattos Jardim , Lacerda Samyra Maria dos Santos Nassif
Published: January 2024

Induced pluripotent stem cells (iPSCs) were first generated by Yamanaka in 2006, revolutionizing research by overcoming limitations imposed by the use of embryonic stem cells. In terms of the conservation of endangered species, iPSC technology presents itself as a viable alternative for the manipulation of target genetics without compromising specimens. Although iPSCs have been successfully generated for various species, their application in nonmammalian species, particularly avian species, requires further in-depth investigation to cover the diversity of wild species at risk and their different protocol requirements. This study aims to provide an overview of the workflow for iPSC induction, comparing well-established protocols in humans and mice with the limited information available for avian species. Here, we discuss the somatic cell sources to be reprogrammed, genetic factors, delivery methods, enhancers, a brief history of achievements in avian iPSC derivation, the main approaches for iPSC characterization, and the future perspectives and challenges for the field. By examining the current protocols and state-of-the-art techniques employed in iPSC generation, we seek to contribute to the development of efficient and species-specific iPSC methodologies for at-risk avian species. The advancement of iPSC technology holds great promise for achieving in vitro germline competency and, consequently, addressing reproductive challenges in endangered species, providing valuable tools for basic research, bird genetic preservation and rescue, and the establishment of cryobanks for future conservation efforts.
An Influx of Non-Native Bird Species into the Natural Environment Owing to the Accidental Release of Pet Birds in Japan
Authors: Nishida Sumiko , Kitamura Wataru
Published: January 2024

The escape of pet birds into the wild raises concerns about the introduction of invasive avian species. This study investigated the impact of escaped pet birds on the introduction of non-native species in Japan. Data sourced from four lost-and-found pet websites between January 2018 and December 2021 revealed 12,125 recorded escapes exhibiting both daily occurrences and seasonal fluctuations. Statistical modeling identified the monthly average temperature (positively correlated) and maximum electricity demand (negatively correlated) as influential factors. Text analysis revealed “window” and “open” as frequently cited reasons for escapes. Budgerigars (Melopsittacus undulatus) and Cockatiels (Nymphicus hollandicus) accounted for 76% of the total escape, suggesting a low perceived risk of establishment in nonnative environments. Interestingly, two globally established invasive species, the Rose-ringed Parakeet (Psittacula krameri) and Monk Parakeet (Myiopsitta monachus), were among the escaped birds. While the Rose-ringed Parakeet is locally naturalized in Tokyo and its adjacent prefectures, the Monk Parakeet failed to establish itself in Japan. Despite the limited number of escaped Monk Parakeets, ongoing efforts are crucial for preventing the potential re-establishment of species with such capabilities.
Toxic Effects of Bisphenol AF Exposure on the Reproduction and Liver of Female Marine Medaka (Oryzias melastigma)
Authors: Li Huichen , Gao Jiahao , Liu Yue , Ding Yujia , Guo Yusong , Wang Zhongduo , Dong Zhongdian , Zhang Ning
Published: January 2024

In recent years, bisphenol AF (BPAF) in aquatic environments has drawn attention to its ecological risks. This study aims to investigate the toxic effects of BPAF (188.33 μg/L) exposure for 30 days on female marine medaka (Oryzias melastigma). On the 10th and 30th day of exposure, the toxicity was evaluated using histological analysis of the liver and ovaries and the transcription levels of genes related to the antioxidant system, immune system, and hypothalamic-pituitary-gonadal (HPG) axis. Findings revealed that (1) BPAF exposure caused vacuolation, karyopyknosis and karyolysis in the liver of marine medaka, and the toxic impact augmented with duration; (2) exposure to BPAF for 10 days facilitated the growth and maturation of primary ova, and this exposure had a comparatively inhibitory effect after 30 days; (3) exposure to BPAF resulted in a biphasic regulation of the transcriptional abundance of genes involved in antioxidant and inflammatory response (e.g., il-8, cat), with an initial up-regulation followed by down-regulation. Additionally, it disrupted the transcriptional pattern of HPG axis-related genes (e.g., 3βhsd, arα). In conclusion, 188.33 μg/L BPAF can alter the expression levels of functionally related genes, impair the structural integrity of marine organisms, and pose a threat to their overall health.
Optimized Antibiotic Management of Critically Ill Patients with Severe Pneumonia Following Multiplex Polymerase Chain Reaction Testing: A Prospective Clinical Exploratory Trial
Authors: Verroken Alexia , Favresse Julien , Anantharajah Ahalieyah , Rodriguez-Villalobos Hector , Wittebole Xavier , Laterre Pierre-François
Published: January 2024

Molecular diagnostic testing is assumed to enable fast respiratory pathogen identification and contribute to improved pneumonia management. We set up a prospective clinical trial at a tertiary hospital intensive care unit including adult patients suspected of severe pneumonia from whom a lower respiratory tract sample could be obtained. During control periods (CPs), routine testing was performed, and during intervention periods (IPs), this testing was completed with the FilmArray Pneumonia Panel plus test (FA-PNEU) executed 24/7. The main objective was to measure the impact of FA-PNEU results in terms of reduced time to targeted antimicrobial treatment administration. Over a 10-month period, analysis was performed on 35 CP and 50 IP patients. The median time to targeted antimicrobial treatment administration was reduced to 4.3 h in IPs compared to 26.4 h in CPs, with 54% of IP patients having FA-PNEU results that led to a treatment modification, of which all but one were targeted. Modifications included 10 (37%) de-escalations, 7 (25.9%) escalations, 3 (11.1%) regimen switches, and 7 (25.9%) complete antimicrobial discontinuations. FA-PNEU results were available with a 42.3 h gain compared to routine identification. This prospective study confirmed retrospective data demonstrating the benefit of FA-PNEU testing in severe pneumonia management of critically ill patients through improved antimicrobial use.
Antibiotic-Coated Intramedullary Nailing Managing Long Bone Infected Non-Unions: A Meta-Analysis of Comparative Studies
Authors: Ghaseminejad-Raeini Amirhossein , Azarboo Alireza , Pirahesh Kasra , Sharafi Amirmohammad , Hoveidaei Amir Human , Nwankwo Basilia Onyinyechukwu , Annasamudram Abhijith , Conway Janet D.
Published: January 2024

Long bone infected non-unions are such an orthopedic challenge that antibiotic-coated intramedullary nailing (ACIN) has become a viable therapeutic option for their management. This study aims to provide a comprehensive assessment of the available data about the use of antibiotic-coated nailing in the treatment of long bone infected non-unions. Following the PRISMA guideline in this meta-analysis, a systematic literature search was conducted across major databases for studies evaluating ACIN in long bone infected non-unions. The primary outcome measures included union rates, infection control, complications and functional status. Five eligible studies encompassing 183 patients in total met the inclusion criteria. The meta-analysis revealed no difference in the union rate in the antibiotic-coated intramedullary nailing group compared to that of the control group (OR = 1.73 [0.75–4.02]). Antibiotic-coated intramedullary nailing demonstrated no association with higher infection eradication (OR = 2.10 [0.97–4.54]). Also, functional outcome measure was mostly not significantly different between ACIN and control interventions. According to this meta-analysis, compared to the management of controls, ACIN is neither linked to increased union rates nor decreased infection rates. The paucity of research on this topic emphasizes the continuous need for additional well-designed randomized controlled trials for the application of antibiotics-coated intramedullary nailing in long bone non-unions.
Methylglyoxal-Derived Nucleoside Adducts Drive Vascular Dysfunction in a RAGE-Dependent Manner
Authors: Lai Seigmund Wai Tsuen , Bhattacharya Supriyo , Lopez Gonzalez Edwin De Jesus , Shuck Sarah C.
Published: January 2024

Diabetic kidney disease (DKD) is a leading cause of death in patients with diabetes. An early precursor to DKD is endothelial cell dysfunction (ECD), which often precedes and exacerbates vascular disease progression. We previously discovered that covalent adducts formed on DNA, RNA, and proteins by the reactive metabolic by-product methylglyoxal (MG) predict DKD risk in patients with type 1 diabetes up to 16 years pre-diagnosis. However, the mechanisms by which MG adducts contribute to vascular disease onset and progression remain unclear. Here, we report that the most predominant MG-induced nucleoside adducts, N2-(1-carboxyethyl)-deoxyguanosine (CEdG) and N2-(1-carboxyethyl)-guanosine (CEG), drive endothelial dysfunction. Following CEdG or CEG exposure, primary human umbilical vein endothelial cells (HUVECs) undergo endothelial dysfunction, resulting in enhanced monocyte adhesion, increased reactive oxygen species production, endothelial permeability, impaired endothelial homeostasis, and exhibit a dysfunctional transcriptomic signature. These effects were discovered to be mediated through the receptor for advanced glycation end products (RAGE), as an inhibitor for intracellular RAGE signaling diminished these dysfunctional phenotypes. Therefore, we found that not only are MG adducts biomarkers for DKD, but that they may also have a role as potential drivers of vascular disease onset and progression and a new therapeutic modality.
A Novel Computational Imaging Algorithm for Electrical Capacitance Tomography
Authors: Zhao Qing , Liu Shi , Chen Weining
Published: January 2024

High-precision images enable electrical capacitance tomography (ECT) to obtain more reliable measurement results, meaning that the reconstruction algorithm is particularly important. Some excellent numerical algorithms have successfully solved the inverse problem for ECT imaging, but their imaging quality is relatively low. To solve this problem, this paper proposes a new reconstruction algorithm based on regularized extreme learning machines (RELMs). The implementation of the algorithm is mainly divided into two steps: (1) according to a large number of training samples, the RELM model can be obtained by the iterative split Bregman (ISB) algorithm, which can describe the mapping relationship between the capacitance correlation coefficient and the imaging target well, and (2) the capacitance correlation coefficient is calculated, which is then used as input to the RELM model to predict the final imaging. Both simulation and experimental results show that the RELM algorithm achieves greater improvement in imaging quality and robustness, and provides new development ideas for the ECT.
Influence of Right Triangular Prism Rough Beds on Hydraulic Jumps
Authors: Trinh Cong Ty , Zhang Jianmin , Tran Cong Trieu
Published: January 2024

A hydraulic jump phenomenon is exciting in turbulent flow as it causes large-scale turbulence and high-energy loss. This paper investigates the hydraulic jump characteristics of right triangular prism rough beds. The renormalization group RNG k-ε turbulent model and the volume of fluid (VOF) method in a CFD model are utilized to simulate hydraulic jumps. A total of 210 numerical simulations of four new types of rough beds were performed with an initial Froude number (Fr1) ranging from 4.8 to 9.4, the non-dimensionless wave steepness values of 0.67 ≤ t/s ≤ 1.33, and the distances between roughness elements of 0 ≤ Ls/y1 ≤ 2.67. This study found that arranging the right triangular prism rough elements in a stilling basin increased bed shear stress and energy loss. At the same time, they reduced sequent depth and jump length by about 22% and 50% compared to a smooth bed, respectively. In addition, the entropy production rates are also used to analyze energy dissipation, which clearly shows that the characteristic shape of a rough bed significantly influences the hydraulic jump length. Equations and plots that specify the relationships between the hydraulic jumps and study parameters are helpful guidelines for defining the rough bed dimension when designing or repairing a stilling basin for low-head irrigation works and highway sewers.
Elucidating Super-Compressible States in Inner Structure of Vehicular Flow
Authors: Krbalek Milan , Krbalkova Michaela
Published: January 2024

The article deals with the open questions in the theory of vehicular headway modeling. Specifically, the question of the existence of anomalous constellations in vehicular traffic micro-structure, in which the rate of fluctuations (measured by the stochastic compressibility) exceeds the fluctuation level of systems with non-interacting elements. The solution to this open problem is converted into the mathematical format working with the so-called balance particle systems, where seeking relevant relations is more straightforward and thus significantly easier. Presented research has shown that unit compressibility represents (despite popular opinion) the upper limit only for particle systems, in which there is no attractive interaction between the particles. In the article, the specific system is constructed in which the presence of an attractive force component will cause higher fluctuations than in the Poisson systems of non-interacting elements. This means that traffic constellations with higher compressibility (so-called super-compressible constellations) can be explained either by a discrepancy between the empirical traffic flow and the mathematical model used, or by the presence of attractive forces acting between individual vehicles. Using empirical vehicular data (measured on two parallel freeway lanes under reconstruction), we show that super-compressible states occur even though overtaking is prohibited. This means, therefore, that these super-compressible states arose without a doubt due to the mutual attraction of successive vehicles. In addition, the article shows that the presence of the aforementioned attractive forces appears predominantly in the fast lane, and only in situations where the traffic density is relatively low. At higher densities, the two freeway lanes are markedly synchronized, the opportunity for a sporty style of driving vanishes and the reason for changing lanes disappears. Under these circumstances, the attractive force component vanishes, which finally leads to the transition of the entire traffic system back to standard sub-compressible states.
Results of Third-Order Strong Differential Subordinations
Authors: Soren Madan Mohan , Wanas Abbas Kareem , Cotîrlǎ Luminiţa-Ioana
Published: January 2024

In this paper, we present and investigate the notion of third-order strong differential subordinations, unveiling several intriguing properties within the context of specific classes of admissible functions. Furthermore, we extend certain definitions, presenting novel and fascinating results. We also derive several interesting properties of the results of third-order strong differential subordinations for analytic functions associated with the Srivastava–Attiya operator.
Comparison between Platelet Lysate, Platelet Lysate Serum, and Fetal Bovine Serum as Supplements for Cell Culture, Expansion, and Cryopreservation
Authors: Duarte Rojas Juan Manuel , Restrepo Múnera Luz Marina , Estrada Mira Sergio
Published: January 2024

As cell culture supplements, human platelet lysate (PL) and human platelet lysate serum (PLS) are alternatives to fetal bovine serum (FBS) due to FBS-related issues such as ethical concerns, variability between batches, and the possible introduction of xenogenic contaminants. This study compared the composition and efficacy of PL, PLS, and FBS as supplements in the culture and cryopreservation of human dermal fibroblasts, Wharton’s jelly-derived mesenchymal stem cells (WJ-MCS), and adipose tissue (AdMSC). Biochemical components, some growth factors, and cytokines present in each of them were analyzed; in addition, the cells were cultured in media supplemented with 5% PL, 5% PLS, and 10% FBS and exposed to different freezing and thawing solutions with the supplements under study. Biochemical parameters were found to be similar in PL and PLS compared to FBS, with some differences in fibrinogen and calcium concentration. Growth factors and cytokines were higher in PL and PLS compared to FBS. Cell proliferation and morphology showed no significant differences between the three culture media. Regarding the cryopreservation and thawing of cells, better results were obtained with PLS and FBS. In conclusion, PL and PLS are an excellent choice to replace the standard supplement of animal origin (FBS) in the media used for the culture and cryopreservation of fibroblasts, WJ-MSC, and AdMSC.
Suppression of Bcl3 Disrupts Viability of Breast Cancer Cells through Both p53-Dependent and p53-Independent Mechanisms via Loss of NF-κB Signalling
Authors: Turnham Daniel J. , Smith Hannah , Clarkson Richard W. E.
Published: January 2024

The NF-κB co-factor Bcl3 is a proto-oncogene that promotes breast cancer proliferation, metastasis and therapeutic resistance, yet its role in breast cancer cell survival is unclear. Here, we sought to determine the effect of Bcl3 suppression alone on breast cancer cell viability, with a view to informing future studies that aim to target Bcl3 therapeutically. Bcl3 was suppressed by siRNA in breast cancer cell lines before changes in viability, proliferation, apoptosis and senescence were examined. Bcl3 suppression significantly reduced viability and was shown to induce apoptosis in all cell lines tested, while an additional p53-dependent senescence and senescence-associated secretory phenotype was also observed in those cells with functional p53. The role of the Bcl3/NF-κB axis in this senescence response was confirmed via siRNA of the non-canonical NF-κB subunit NFKB2/p52, which resulted in increased cellular senescence and the canonical subunit NFKB1/p50, which induced the senescence-associated secretory phenotype. An analysis of clinical data showed a correlation between reduced relapse-free survival in patients that expressed high levels of Bcl3 and carried a p53 mutation. Together, these data demonstrate a dual role for Bcl3/NF-κB in the maintenance of breast cancer cell viability and suggests that targeting Bcl3 may be more beneficial to patients with tumours that lack functional p53.
Unraveling the Role of miR-200b-3p in Attention-Deficit/Hyperactivity Disorder (ADHD) and Its Therapeutic Potential in Spontaneously Hypertensive Rats (SHR)
Authors: Chang Tung-Ming , Lin Hsiu-Ling , Tzang Chih-Chen , Liang Ju-An , Hsu Tsai-Ching , Tzang Bor-Show
Published: January 2024

Attention-deficit/hyperactivity disorder (ADHD) is a prevalent neurodevelopmental disorder in children with unknown etiology. Impaired learning ability was commonly reported in ADHD patients and has been associated with dopamine uptake in the striatum of an animal model. Another evidence also indicated that micro-RNA (miR)-200b-3p is associated with learning ability in various animal models. However, the association between miR-200b-3p and ADHD–related symptoms remains unclear. Therefore, the current study investigated the role of miR-200b-3p in ADHD-related symptoms such as inattention and striatal inflammatory cytokines. To verify the influence of miR-200b-3p in ADHD-related symptoms, striatal stereotaxic injection of miR-200b-3p antagomir (AT) was performed on spontaneously hypertensive rats (SHR). The antioxidant activity and expressions of miR-200b-3p, slit guidance ligand 2 (Slit2), and inflammatory cytokines in the striatum of SHR were measured using quantitative real-time polymerase chain reaction (RT-qPCR), immunohistochemistry (IHC), immunoblotting, and enzyme-linked immunosorbent assay (ELISA). The spontaneous alternation of SHR was tested using a three-arm Y-shaped maze. The administration of miR-200b-3p AT or taurine significantly decreased striatal tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in SHR, along with increased super-oxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and significantly higher spontaneous alternation. In this paper, we show that miR-200b-3p AT and taurine alleviates ADHD-related symptoms in SHR. These findings provide insights into ADHD’s molecular basis and suggest miR-200b-3p as a potential therapeutic target. Concurrently, this study also suggests broad implications for treating neurodevelopmental disorders affecting learning activity such as ADHD.
Accelerated Brain Atrophy, Microstructural Decline and Connectopathy in Age-Related Macular Degeneration
Authors: Stout Jacques A. , Mahzarnia Ali , Dai Rui , Anderson Robert J. , Cousins Scott , Zhuang Jie , Lad Eleonora M. , Whitaker Diane B. , Madden David J. , Potter Guy G. , Whitson Heather E. , Badea Alexandra
Published: January 2024

Age-related macular degeneration (AMD) has recently been linked to cognitive impairment. We hypothesized that AMD modifies the brain aging trajectory, and we conducted a longitudinal diffusion MRI study on 40 participants (20 with AMD and 20 controls) to reveal the location, extent, and dynamics of AMD-related brain changes. Voxel-based analyses at the first visit identified reduced volume in AMD participants in the cuneate gyrus, associated with vision, and the temporal and bilateral cingulate gyrus, linked to higher cognition and memory. The second visit occurred 2 years after the first and revealed that AMD participants had reduced cingulate and superior frontal gyrus volumes, as well as lower fractional anisotropy (FA) for the bilateral occipital lobe, including the visual and the superior frontal cortex. We detected faster rates of volume and FA reduction in AMD participants in the left temporal cortex. We identified inter-lingual and lingual–cerebellar connections as important differentiators in AMD participants. Bundle analyses revealed that the lingual gyrus had a lower streamline length in the AMD participants at the first visit, indicating a connection between retinal and brain health. FA differences in select inter-lingual and lingual cerebellar bundles at the second visit showed downstream effects of vision loss. Our analyses revealed widespread changes in AMD participants, beyond brain networks directly involved in vision processing.
Detection of Sialic Acid to Differentiate Cervical Cancer Cell Lines Using a Sambucus nigra Lectin Biosensor
Authors: Zamudio Cañas Ricardo , Jaramillo Flores María Eugenia Jaramillo , Vallejo Ruiz Verónica , Delgado Macuil Raúl Jacobo , López Gayou Valentín
Published: January 2024

Pap smear screening is a widespread technique used to detect premalignant lesions of cervical cancer (CC); however, it lacks sensitivity, leading to identifying biomarkers that improve early diagnosis sensitivity. A characteristic of cancer is the aberrant sialylation that involves the abnormal expression of α2,6 sialic acid, a specific carbohydrate linked to glycoproteins and glycolipids on the cell surface, which has been reported in premalignant CC lesions. This work aimed to develop a method to differentiate CC cell lines and primary fibroblasts using a novel lectin-based biosensor to detect α2,6 sialic acid based on attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and chemometric. The biosensor was developed by conjugating gold nanoparticles (AuNPs) with 5 µg of Sambucus nigra (SNA) lectin as the biorecognition element. Sialic acid detection was associated with the signal amplification in the 1500–1350 cm−1 region observed by the surface-enhanced infrared absorption spectroscopy (SEIRA) effect from ATR-FTIR results. This region was further analyzed for the clustering of samples by applying principal component analysis (PCA) and confidence ellipses at a 95% interval. This work demonstrates the feasibility of employing SNA biosensors to discriminate between tumoral and non-tumoral cells, that have the potential for the early detection of premalignant lesions of CC.
Effect of Dancing Interventions on Depression and Anxiety Symptoms in Older Adults: A Systematic Review and Meta-Analysis
Authors: Prudente Tiago Paiva , Mezaiko Eleazar , Silveira Erika Aparecida , Nogueira Túlio Eduardo
Published: January 2024

This systematic review and meta-analysis (PROSPERO CRD42023428105) investigated the effect of dancing on depression and anxiety symptoms in older adults. Conducted up to October 2023, the search across seven databases and gray literature yielded 5020 records. Only randomized trials that analyzed dance interventions for depression and/or anxiety in older adults were included. Nineteen randomized trials, involving 508 participants in dance classes lasting 5 weeks to 18 months, were included and 16 were subjected to meta-analysis. Risk of bias was assessed using the Cochrane tool. The meta-analysis showed a statistically significant reduction in depression among older adults participating in dance interventions (p < 0.01). A decrease in depressive symptoms was significant compared to that in those involved in no other intervention (p = 0.02) but not compared to that achieved with other interventions in control groups (p = 0.96). Subgroup analysis showed no significant differences in depression scores for those with mild cognitive impairment (p = 0.47). These conclusions are associated with moderate bias and very low certainty. Due to heterogeneity and the small number of studies, conclusions for anxiety outcomes could not be drawn. These results underscore the potential clinical relevance of integrating dance into mental health interventions for older adults, thereby highlighting a promising avenue for enhancing the mental well-being of this demographic.
Thermal Performance of Residential Roofs in Malaysia: Experimental Study Using an Indoor Solar Simulator
Authors: Mohd Ashhar Muhamad Zahin , Lim Chin Haw
Published: January 2024

Previous researchers have detailed the problems in measuring the thermal resistance value of a whole roof assembly under hot conditions due to the uncertainty of the outdoor environment. Currently, no established method exists to experimentally investigate an entire thermal roof performance under a steady-state condition. This article details the properties of the indoor solar simulator and the research methods undertaken to measure the thermal resistance value of roof assembly. The indoor solar simulator utilizes 40 halogen bulbs to accurately replicate sun radiation. Thermocouples and heat flux sensors are installed at several locations on the roof assembly to quantify the heat transmission occurring through it. The thermal resistance value is determined by adding up the average difference in temperature across the external and internal roof surfaces and dividing the total amount by the total of all averaged heat fluxes. Subsequently, this study investigates the thermal efficiency of residential roof assemblies that comprise various insulation materials frequently employed in Malaysia, including stone wool, mineral glass wool, reflective bubble foil insulation, and radiant barriers. The analysis showed that the roof configurations with bubble foil reflective insulation produce superior thermal resistance values when coupled with enclosed air space or mass insulation, with thermal resistance values ranging between 2.55 m2K/W and 3.22 m2K/W. It can be concluded that roof configurations with bubble foil reflective insulation resulted in high total thermal resistance and passed the minimum thermal resistance value of 2.5 m2K/W under the Malaysian Uniform Building By-Law 38 (A) requirements. Furthermore, the radiant barrier produced a high thermal resistance value of 2.50 m2K/W when installed parallel to a 50 mm enclosed air space, emphasising the crucial function of an enclosed air space next to a reflective foil to resist the incoming heat radiation. The findings from this research can help building professionals determine the optimum insulation for residential building roofs in Malaysia.
Navigating Post-Operative Outcomes: A Comprehensive Reframing of an Original Graded Prognostic Assessment in Patients with Brain Metastases
Authors: Goldberg Maria , Mondragon-Soto Michel G. , Dieringer Laura , Altawalbeh Ghaith , Pöser Paul , Baumgart Lea , Wiestler Benedikt , Gempt Jens , Meyer Bernhard , Aftahy Amir Kaywan
Published: January 2024

Background: Graded Prognostic Assessment (GPA) has been proposed for various brain metastases (BMs) tailored to the primary histology and molecular profiles. However, it does not consider whether patients have been operated on or not and does not include surgical outcomes as prognostic factors. The residual tumor burden (RTB) is a strong predictor of overall survival. We validated the GPA score and introduced “volumetric GPA” in the largest cohort of operated patients and further explored the role of RTB as an additional prognostic factor. Methods: A total of 630 patients with BMs between 2007 and 2020 were included. The four GPA components were analyzed. The validity of the original score was assessed using Cox regression, and a modified index incorporating RTB was developed by comparing the accuracy, sensitivity, specificity, F1-score, and AUC parameters. Results: GPA categories showed an association with survival: age (p < 0.001, hazard ratio (HR) 2.9, 95% confidence interval (CI) 2.5–3.3), Karnofsky performance status (KPS) (p < 0.001, HR 1.3, 95% CI 1.2–1.5), number of BMs (p = 0.019, HR 1.4, 95% CI 1.1–1.8), and the presence of extracranial manifestation (p < 0.001, HR 3, 95% CI 1.6–2.5). The median survival for GPA 0–1 was 4 months; for GPA 1.5–2, it was 12 months; for GPA 2.5–3, it was 21 months; and for GPA 3.5–4, it was 38 months (p < 0.001). RTB was identified as an independent prognostic factor. A cut-off of 2 cm3 was used for further analysis, which showed a median survival of 6 months (95% CI 4–8) vs. 13 months (95% CI 11–14, p < 0.001) for patients with RTB > 2 cm3 and <2 cm3, respectively. RTB was added as an additional component for a modified volumetric GPA score. The survival rates with the modified GPA score were: GPA 0–1: 4 months, GPA 1.5–2: 7 months, GPA 2.5–3: 18 months, and GPA 3.5–4: 34 months. Both scores showed good stratification, with the new score showed a trend towards better discrimination in patients with more favorable prognoses. Conclusion: The prognostic value of the original GPA was confirmed in our cohort of patients who underwent surgery for BM. The RTB was identified as a parameter of high prognostic significance and was incorporated into an updated “volumetric GPA”. This score provides a novel tool for prognosis and clinical decision making in patients undergoing surgery. This method may be useful for stratification and patient selection for further treatment and in future clinical trials.
The Tumor Suppressor SOCS1 Diminishes Tolerance to Oxidative Stress in Hepatocellular Carcinoma
Authors: Shukla Akhil , Khan Md Gulam Musawwir , Cayarga Anny Armas , Namvarpour Mozhdeh , Chowdhury Mohammad Mobarak H. , Levesque Dominique , Lucier Jean-François , Boisvert François-Michel , Ramanathan Sheela , Ilangumaran Subburaj
Published: January 2024

SOCS1 is a tumor suppressor in hepatocellular carcinoma (HCC). Recently, we showed that a loss of SOCS1 in hepatocytes promotes NRF2 activation. Here, we investigated how SOCS1 expression in HCC cells affected oxidative stress response and modulated the cellular proteome. Murine Hepa1-6 cells expressing SOCS1 (Hepa-SOCS1) or control vector (Hepa-Vector) were treated with cisplatin or tert-butyl hydroperoxide (t-BHP). The induction of NRF2 and its target genes, oxidative stress, lipid peroxidation, cell survival and cellular proteome profiles were evaluated. NRF2 induction was significantly reduced in Hepa-SOCS1 cells. The gene and protein expression of NRF2 targets were differentially induced in Hepa-Vector cells but markedly suppressed in Hepa-SOCS1 cells. Hepa-SOCS1 cells displayed an increased induction of reactive oxygen species but reduced lipid peroxidation. Nonetheless, Hepa-SOCS1 cells treated with cisplatin or t-BHP showed reduced survival. GCLC, poorly induced in Hepa-SOCS1 cells, showed a strong positive correlation with NFE2L2 and an inverse correlation with SOCS1 in the TCGA-LIHC transcriptomic data. A proteomic analysis of Hepa-Vector and Hepa-SOCS1 cells revealed that SOCS1 differentially modulated many proteins involved in diverse molecular pathways, including mitochondrial ROS generation and ROS detoxification, through peroxiredoxin and thioredoxin systems. Our findings indicate that maintaining sensitivity to oxidative stress is an important tumor suppression mechanism of SOCS1 in HCC.
Gαi2 Protein Inhibition Blocks Chemotherapy- and Anti-Androgen-Induced Prostate Cancer Cell Migration
Authors: Caggia Silvia , Johnston Alexis , Walunj Dipak T. , Moore Aanya R. , Peer Benjamin H. , Everett Ravyn W. , Oyelere Adegboyega K. , Khan Shafiq A.
Published: January 2024

We have previously shown that heterotrimeric G-protein subunit alphai2 (Gαi2) is essential for cell migration and invasion in prostate, ovarian and breast cancer cells, and novel small molecule inhibitors targeting Gαi2 block its effects on migratory and invasive behavior. In this study, we have identified potent, metabolically stable, second generation Gαi2 inhibitors which inhibit cell migration in prostate cancer cells. Recent studies have shown that chemotherapy can induce the cancer cells to migrate to distant sites to form metastases. In the present study, we determined the effects of taxanes (docetaxel), anti-androgens (enzalutamide and bicalutamide) and histone deacetylase (HDAC) inhibitors (SAHA and SBI-I-19) on cell migration in prostate cancer cells. All treatments induced cell migration, and simultaneous treatments with new Gαi2 inhibitors blocked their effects on cell migration. We concluded that a combination treatment of Gαi2 inhibitors and chemotherapy could blunt the capability of cancer cells to migrate and form metastases.
Coronavirus M Protein Trafficking in Epithelial Cells Utilizes a Myosin Vb Splice Variant and Rab10
Authors: Lapierre Lynne A. , Roland Joseph T. , Manning Elizabeth H. , Caldwell Catherine , Glenn Honor L. , Vidalain Pierre-Olivier , Tangy Frederic , Hogue Brenda G. , de Haan C. A. M. , Goldenring James R.
Published: January 2024

The membrane (M) glycoprotein of coronaviruses (CoVs) serves as the nidus for virion assembly. Using a yeast two-hybrid screen, we identified the interaction of the cytosolic tail of Murine Hepatitis Virus (MHV-CoV) M protein with Myosin Vb (MYO5B), specifically with the alternative splice variant of cellular MYO5B including exon D (MYO5B+D), which mediates interaction with Rab10. When co-expressed in human lung epithelial A549 and canine kidney epithelial MDCK cells, MYO5B+D co-localized with the MHV-CoV M protein, as well as with the M proteins from Porcine Epidemic Diarrhea Virus (PEDV-CoV), Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome 2 (SARS-CoV-2). Co-expressed M proteins and MYO5B+D co-localized with endogenous Rab10 and Rab11a. We identified point mutations in MHV-CoV M that blocked the interaction with MYO5B+D in yeast 2-hybrid assays. One of these point mutations (E121K) was previously shown to block MHV-CoV virion assembly and its interaction with MYO5B+D. The E to K mutation at homologous positions in PEDV-CoV, MERS-CoV and SARS-CoV-2 M proteins also blocked colocalization with MYO5B+D. The knockdown of Rab10 blocked the co-localization of M proteins with MYO5B+D and was rescued by re-expression of CFP-Rab10. Our results suggest that CoV M proteins traffic through Rab10-containing systems, in association with MYO5B+D.
Complementary Feeding: From Tradition to Personalized Nutrition
Authors: Panza Raffaella , Baldassarre Maria Elisabetta
Published: January 2024

The introduction of solid foods into the infant’s diet is a moment of great change in the routine of parents and children [...]
Disease Trends in Children and Adolescents in Japan: A Retrospective Observational Study Using the Nationwide Claims Data for 2012–2016
Authors: Suto Maiko , Takehara Kenji , Morisaki Naho , Moriichi Akinori , Gai Ruoyan , Mori Rintaro
Published: January 2024

This study aimed to clarify diseases that occur more frequently by age and identify the peaks and trends of each disease from infancy to adolescence for early detection and treatment. This retrospective observational study was conducted using Japan’s National Database of Health Insurance Claims Specific Health Checkups from January 2012 to December 2016. Using peak ages and trends in the number of patients, we grouped diseases by the International Classification of Diseases chapters. Although diseases that peaked during infancy were the most common (10 disease chapters), other diseases peaked at school-going age and adolescence. Diseases in four chapters peaked during adolescence and continued to increase toward the age of 18. These four chapters included mental, behavioral, and neurodevelopmental disorders; diseases of the nervous system; the genitourinary system; and pregnancy, childbirth, and the puerperium. Childhood-onset diseases can affect long-term health and healthcare needs, and timely screening and guidance based on disease trends can provide an effective intervention. To establish a child healthcare system that provides preventive support for children and adolescents’ physical, psychological, and social health, further research is needed to comprehensively understand the issues per age and developmental stage.
Neonatal Airway Management
Authors: Pinheiro Joaquim M. B.
Published: January 2024

The neonatal airway is often difficult to secure, whether the practitioner responsible for managing the airway is a neonatologist, pediatrician, anesthesiologist, another specialist or an advanced practice provider [...]
Analysis and Mechanism Study of Residual Stress during the Spontaneous Crystallisation Process of Molten Titanium-Containing Blast Furnace Slag
Authors: Wang Daizheng , Yan Bingji , Dang Ziyu , Li Peng , Guo Hongwei , Song Ziyu
Published: January 2024

Molten titanium-containing blast furnace slag can be used to obtain cast stone materials by controlling a reasonable heat treatment system. The material acquired during this process showcases residual stress, which additionally impacts the macroscopic characteristics of the material. This article simulates the process of manufacturing microcrystalline cast stones based on the self-crystallisation ability of titanium-containing products. This research employs X-ray diffraction to precisely and conveniently assess the residual stress of microcrystalline cast stones and investigates how viscosity and the thermal expansion coefficient influence the residual stress level. The study provides a theoretical foundation for explaining titanium-containing blast furnace slag and combines characterisation methods such as XRD (X-ray diffraction), SEM (Scanning electron microscope), DTA (Differential thermal analysis), and theoretical calculations such as Factpage and Fullprop to study the effect of the TiO2 content on the microstructure of self-crystallised mechanical characteristics of microcrystalline cast stones through residual stress. The results of the experiment indicate that as the TiO2 content in the system increases, the glass phase is reduced, the crystallinity improves, and the main crystal phase changes from a feldspar phase to a diopside phase. Furthermore, its viscosity, thermal expansion coefficient, and residual stress decrease while its corresponding compressive strength and bending strength increase.
Adoption of Total Neoadjuvant Therapy in the Treatment of Locally Advanced Rectal Cancer
Authors: Conces Madison L. , Mahipal Amit
Published: January 2024

Local and metastatic recurrence are primary concerns following the treatment of locally advanced rectal cancer (LARC). Chemoradiation (CRT) can reduce the local recurrence rates and has subsequently moved to the neoadjuvant setting from the adjuvant setting. Pathological complete response (pCR) rates have also been noted to be greater in patients treated with neoadjuvant CRT prior to surgery. The standard approach to treating LARC would often involve CRT followed by surgery and optional adjuvant chemotherapy and remained the treatment paradigm for almost two decades. However, patients were often unable to complete adjuvant chemotherapy due to a decreased tolerance of chemotherapy following surgery, which led to upfront treatment with both CRT and chemotherapy, and total neoadjuvant therapy, or TNT, was created. The efficacy outcomes of local recurrence, disease-free survival, and pCR have improved in patients receiving TNT compared to the standard approach. Additionally, more recent data suggest a possible improvement in overall survival as well. Patients with a complete clinical response following TNT have the opportunity for watch-and-wait surveillance, allowing some patients to undergo organ preservation. Here, we discuss the clinical trials and studies that led to the adoption of TNT as the standard of care for LARC, with the possibility of watch-and-wait surveillance for patients achieving complete responses. We also review the possibility of overtreating some patients with LARC.
A Quantitative Evaluation of the Effectiveness of the Metal Artifact Reduction Algorithm in Cone Beam Computed Tomographic Images with Stainless Steel Orthodontic Brackets and Arch Wires: An Ex Vivo Study
Authors: Shavakhi Mojgan , Soltani Parisa , Aghababaee Golnaz , Patini Romeo , Armogida Niccolò Giuseppe , Spagnuolo Gianrico , Valletta Alessandra
Published: January 2024

The presence of high-density and high-atomic number materials results in the generation of artifacts in cone beam computed tomographic (CBCT) images. To minimize artifacts in CBCT images, the metal artifact reduction (MAR) tool was developed. This study aims to quantitatively evaluate the effectiveness of the MAR algorithm in CBCT images of teeth with stainless steel orthodontic brackets with or without arch wires in buccal and lingual positions obtained using the Galileos Sirona CBCT scanner. In this in vitro study, 20 stainless steel brackets were attached to the maxillary dentition from the right second premolar to the left second premolar teeth of a human skull. In the first group, 10 brackets were bonded to the buccal surface, and in the second group, 10 brackets were bonded to the palatal surface of these teeth. CBCT scans were obtained for each group with or without orthodontic stainless steel wires using a Galileos Sirona CBCT scanner with exposure parameters of 85 kVp and 21 mAs. CBCT images were obtained two times with and two times without MAR activation. The DICOM format of the CBCT images was imported to ImageJ software (version 1.54), and the contrast-to-noise ratio (CNR) was calculated and compared for each bracket in 15 and 20 mm distances and 20, 40, and 90 degrees on each side. Statistical analysis was performed using the t test (α = 0.05). CNR values of different distances and different teeth were not significantly different between the two MAR modes (p > 0.05). MAR activation had a significant impact in increasing CNR and reducing artifacts only when brackets were in palatal (p = 0.03). In the other bracket and wire positions, the effect of the MAR algorithm on CNR was not significant (p > 0.05). In conclusion, MAR activation significantly increased CNR, but only when the brackets were in a palatal position. In the other bracket and wire positions, the effect of the MAR algorithm is not significant.
Wind Tunnel Balance Measurements of Bioinspired Tails for a Fixed Wing MAV
Authors: Bardera Rafael , Rodríguez-Sevillano Ángel. A. , Barroso Estela , Matías Juan Carlos , Sor Mendi Suthyvann
Published: January 2024

Bird tails play a significant role in aerodynamics and stability during flight. This paper investigates the use of bioinspired horizontal stabilizers for Micro Air Vehicles (MAVs) with Zimmerman wing-body geometry. Five configurations of bioinspired horizontal stabilizers are presented. Then, 3-component external balance force measurements of each horizontal stabilizer are performed in the wind tunnel. The Squared-Fan-Shaped Horizontal Stabilizer (HSF-tail) is selected as the optimal horizontal stabilizer that provides the highest aerodynamic efficiency during cruise flight while maintaining high longitudinal stability on the vehicle. The integration of the HSF-tail increases the aerodynamic efficiency by more than 6% up to a maximum of 17% compared to the other alternatives while maintaining the lowest aerodynamic drag value during the cruise phase. Furthermore, balance measurements to analyze the influence of the HSF-tail deflection on the aerodynamic coefficients are conducted, resulting in increased lift force and reduced aerodynamic drag with negative tail deflections. Lastly, the experimental data is validated with CFD-RANS steady simulations for low angles of attack, obtaining a relative difference on the measurement around 5% for the aerodynamic drag coefficient and around 10% for the lift coefficient during the cruise flight that demonstrates a high degree of accuracy in the aerodynamic coefficients obtained by external balance in the wind tunnel. This work represents a novel approach through the implementation of a horizontal stabilizer inspired by the structure of the tails of birds that is expected to yield significant advancements in both stability and aerodynamic efficiency, with the potential to revolutionize MAV technology.
Non-Symmetry in the Shock Refraction at a Closed Interface as a Recovery Mechanism
Authors: Markhotok Anna
Published: January 2024

The possibility of a shock wave recovery at a discrete closed interface with a heated gas has been investigated. A two-dimensional model applied to conditions of optical discharges featuring spherical, elliptical, and drop-like configurations demonstrated that non-symmetry in the shock refraction contributes to the specific mechanism of recovery other than simply its compensation. Even though the full restoration of the hypersonic flow state does not occur in a strict sense of it, clear reverse changes toward the initial shape of the shock front eventually take place, thus creating an appearance of a full recovery seen in experiments. From analysis of different interface symmetries, the factors determining the recovery dynamics are identified. The results are directly applicable to the problem of energy deposition into a hypersonic flow; however, it can be useful anywhere else where the flow modifications following the interaction are important. The dimensionless form of the equations allows applications on any scale other than that demonstrated for the optical discharges.
Complexity and Statistical Physics Approaches to Earthquakes
Authors: Michas Georgios
Published: January 2024

This Special Issue of Entropy, “Complexity and Statistical Physics Approaches to Earthquakes”, sees the successful publication of 11 original scientific articles [...]