bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated transcriptome and microRNA sequencing analyses reveal gene responses in poplar leaves infected by the novel pathogen bean common mosaic virus (BCMV)
Authors: Wang Li , Zhang Weixi , Shen Wanna , Li Min , Fu Yuchen , Li Zheng , Li Jinxin , Liu Huixiang , Su Xiaohua , Zhang Bingyu , Zhao Jiaping
Published: June 2023

Recently, a novel poplar mosaic disease caused by bean common mosaic virus (BCMV) was investigated in Populus alba var. pyramidalis in China. Symptom characteristics, physiological performance of the host, histopathology, genome sequences and vectors, and gene regulation at the transcriptional and posttranscriptional levels were analyzed and RT−qPCR (quantitative reverse transcription PCR) validation of expression was performed in our experiments. In this work, the mechanisms by which the BCMV pathogen impacts physiological performance and the molecular mechanisms of the poplar response to viral infection were reported. The results showed that BCMV infection decreased the chlorophyll content, inhibited the net photosynthesis rate (Pn) and stomatal conductance (Gs), and significantly changed chlorophyll fluorescence parameters in diseased leaves. Transcriptome analysis revealed that the expression of the majority of DEGs (differentially expressed genes) involved in the flavonoid biosynthesis pathway was promoted, but the expression of all or almost all DEGs associated with photosynthesis-antenna proteins and the photosynthesis pathway was inhibited in poplar leaves, suggesting that BCMV infection increased the accumulation of flavonoids but decreased photosynthesis in hosts. Gene set enrichment analysis (GSEA) illustrated that viral infection promoted the expression of genes involved in the defense response or plant-pathogen interaction. MicroRNA-seq analysis illustrated that 10 miRNA families were upregulated while 6 families were downregulated in diseased poplar leaves; moreover, miR156, the largest family with the most miRNA members and target genes, was only differentially upregulated in long-period disease (LD) poplar leaves. Integrated transcriptome and miRNA-seq analyses revealed 29 and 145 candidate miRNA−target gene pairs; however, only 17 and 76 pairs, accounting for 2.2% and 3.2% of all DEGs, were authentically negatively regulated in short-period disease (SD) and LD leaves, respectively. Interestingly, 4 miR156/SPL (squamosa promoter-binding-like protein) miRNA−target gene pairs were identified in LD leaves: the miR156 molecules were upregulated, but SPL genes were downregulated. In conclusion, BCMV infection significantly changed transcriptional and posttranscriptional gene expression in poplar leaves, inhibited photosynthesis, increased the accumulation of flavonoids, induced systematic mosaic symptoms, and decreased physiological performance in diseased poplar leaves. This study elucidated the fine-tuned regulation of poplar gene expression by BCMV; moreover, the results also suggested that miR156/SPL modules played important roles in the virus response and development of viral systematic symptoms in plant virus disease.
Geographical patterns and determinants in plant reproductive phenology duration
Authors: Wang Xinyang , Morin Xavier , Zhang Jian , Chen Guoke , Mao Lingfeng , Chen Yuheng , Song Zhuqiu , Du Yanjun , Ma Keping
Published: June 2023

Biodiversity is and always has been an important issue in ecological research. Biodiversity can reflect niche partitioning among species at several spatial and temporal scales and is generally highest in the tropics. One theory to explain it is that low-latitude tropical ecosystems are dominated by species that are generally only distributed over a narrow area. This principle is known as Rapoport’s rule. One previously unconsidered extension of Rapoport’s rule may be reproductive phenology, where variation in flowering and fruiting length may reflect a temporal range. Herein, we collected reproductive phenology data for more than 20,000 species covering almost all angiosperm species in China. We used a random forest model to quantify the relative role of seven environmental factors on the duration of reproductive phenology. Our results showed that the duration of reproductive phenology decreased with latitude, although there was no obvious change across longitudes. Latitude explained more of the variation in the duration of flowering and fruiting phases in woody plants than in herbaceous plants. Mean annual temperature and the length of the growing season strongly influenced the phenology of herbaceous plants, and average winter temperature and temperature seasonality were important drivers of woody plant phenology. Our result suggests the flowering period of woody plants is sensitive to temperature seasonality, while it does not influence herbaceous plants. By extending Rapoport’s rule to consider the distribution of species in time as well as space, we have provided a novel insight into the mechanisms of maintaining high levels of diversity in low-latitude forests.
Analyzing and supporting mental representations and strategies in solving Bayesian problems
Authors: Sirock Julia , Vogel Markus , Seufert Tina
Published: June 2023

Solving Bayesian problems poses many challenges, such as identifying relevant numerical information, classifying, and translating it into mathematical formula language, and forming a mental representation. This triggers research on how to support the solving of Bayesian problems. The facilitating effect of using numerical data in frequency format instead of probabilities is well documented, as is the facilitating effect of given visualizations of statistical data. The present study not only compares the visualizations of the 2 × 2 table and the unit square, but also focuses on the results obtained from the self-creation of these visualizations by the participants. Since it has not yet been investigated whether the better correspondence between external and internal visualization also has an effect on cognitive load when solving Bayesian tasks, passive and active cognitive load are additionally measured. Due to the analog character and the proportional representation of the numerical information by the unit square, it is assumed that the passive cognitive load is lower when using the unit square as visualization than when using the 2 × 2 table. The opposite is true for active cognitive load.
Combatting negative bias: a mental contrasting and implementation intentions online intervention to increase help-seeking among individuals with elevated depressive symptomatology
Authors: Keeler Amanda R. , Nydegger Liesl A. , Crano William D.
Published: June 2023

Background

There are many reasons why individuals with depression may not seek help. Among those with elevated depressive symptomatology, some previous interventions aimed at increasing help-seeking have unintentionally decreased help-seeking intentions. Beck’s cognitive theory of depression posits that individuals with elevated depressive symptomatology process information differently from those without depression (i.e., increased cognitive errors, negative bias); potentially explaining the iatrogenic results of previous interventions. Mental contrasting and implementation intentions (MCII; a self-regulatory strategy) interventions have successfully influenced physical and mental health behaviors. However, MCII has not been used specifically for initiating help-seeking for depression. The goal of this research was to ascertain whether an online MCII intervention could increase actual help-seeking or the intention to seek help for depression.

Method

Two online randomized pre-post experiments were conducted to measure the primary outcome measures 2 weeks post-intervention (Study 1 collected Summer 2019: information-only control [“C”], help-seeking MCII intervention [“HS”], and comparison MCII intervention [“E”]; Study 2 collected Winter 2020: “C” and “HS”). At Time 1, adults recruited from MTurk had a minimum Beck Depression Inventory (BDI-II) score of 14 (mild depressive symptoms) and were not seeking professional help.

Results

Study 1 (N = 74) indicated that the intervention was feasible, provided preliminary support, and clarified intervention components for Study 2. Study 2 (N = 224) indicated that the HS group reported greater intentions to seek help and actual help-seeking than the C group. Proportionally, actual help-seeking was more likely among individuals who received the HS intervention and either did not perceive themselves as depressed at Time 2 or had BDI-II scores indicating that their depressive symptomatology decreased from Time 1.

Limitations

Participation was limited to US residents who self-reported data.

Discussion

These studies indicate that a brief online MCII intervention to encourage help-seeking is feasible and preliminarily successful. Future studies should consider using ecological momentary assessment measurements to establish the temporal precedence of intervention effects and whether MCII is effective for encouraging help-seeking among individuals prone to experiencing cognitive errors who may not be experiencing negative bias (e.g., bipolar disorder or anxiety). Clinicians may find this method successful in encouraging ongoing treatment engagement.
Effects of verbal tasks with varying difficulty on real-time respiratory airflow during speech generation in healthy young adults
Authors: Gullsvåg Malin , Rodríguez-Aranda Claudia
Published: June 2023

Objective

Respiratory function is linked to sensory, affective, and cognitive processes and it is affected by environmental constraints such as cognitive demands. It is suggested that specific cognitive processes, such as working memory or executive functioning, may impact breathing. In turn, various lines of research have suggested a link between peak expiratory airflow (PEF) and cognitive function. However, there is scarce experimental support to the above assertions, especially regarding spoken language. Therefore, the present investigation aims to evaluate whether breathing varies as a function of performing verbal naming tasks with different difficulty levels.

Methods

Thirty healthy young adults, (age M = 25.37 years), participated in the study. Participants were required to perform aloud five verbal tasks ranged in order of difficulty: Reading single words, reading a text passage, object naming, semantic and phonemic fluency. A pneumotachograph mask was employed to acquire simultaneously the verbal responses, and three airflow parameters: Duration, peak, and volume at both stages of the respiratory cycle (i.e., inspiration/expiration). Data were analyzed with one-way repeated measures MANOVA.

Results

No significant differences were found between reading single words and object naming. In comparison, distinctive airflow requirements were found for reading a text passage, which were proportionally related to number of pronounced words. Though, the main finding of the study concerns the data on verbal fluency tasks, which not only entailed higher inhaled airflow resources but also a significant PEF.

Conclusion

Our data demonstrated that the most difficult tasks, namely semantic and phonemic verbal fluencies, relying on semantic search, executive function, and fast lexical retrieval of words were those requiring important amount of inhaled airflow and displaying a high peak expiratory airflow. The present findings demonstrated for the first time a direct association between complex verbal tasks and PEF. Inconclusive data related to object naming and reading single words are discussed in light of the methodological challenges inherent to the assessment of speech breathing and cognition in this line of investigation.
Does multisensory stimulation with virtual reality (VR) and smell improve learning? An educational experience in recall and creativity
Authors: Andonova Veneta , Reinoso-Carvalho Felipe , Jimenez Ramirez Manuel Arturo , Carrasquilla David
Published: June 2023

Purpose

The purpose of this paper is to derive into practical recommendations from multisensory stimulation with virtual reality (VR) and scent to help educators develop effective teaching strategies geared toward aspects of the learning experience, recall, and creativity in a stereotypical learning context.

Design/methodology/approach

The paper is based on a randomized experiment in which student participants were subdivided into three treatment groups and one control group. Each group was stimulated by a different combination of visual, auditory, and olfactory stimuli (2D SMELL, VR, and VR SMELL) and the outcomes were compared against those of the control group (2D). Consistent with the Cognitive Theory of Multimedia Learning, hypotheses were constructed to study the effect of different combinations of stimuli on the learning experience and learning outcomes related to recall and creativity in a stereotypical learning context.

Findings

Traditional video content alone and bundled with a coherent olfactory stimulus prompted higher self-reported ratings of perceived quality of the sensory experience. Olfactory stimulus in combination with either VR or a traditional video prompted higher self-reported ratings on perceived immersion. In a stereotypical learning context, the highest recall scores were achieved with traditional video alone. Both VR alone and bundled with an olfactory stimulus resulted in enhanced creativity.

Research limitations/implications

The findings of this study should be interpreted in the context of adopting multisensory stimulations combined with VR technology as part of stereotypical learning contexts. Most professional educators do not have robust knowledge or experience in using build-on-purpose multisensory stimuli but are increasingly engaged in using multisensory tools such as VR, as part of their teaching practice. In relation to recall, the results are consistent with the hypothesis that in a stereotypical learning context, a multisensory experience involving VR and olfactory stimuli can be related to an undesired cognitive load for learners. There exists a possibility that the low-technical version of the VR goggles used, as well as the contents of the instructional video may have influenced the learning outcomes in terms of recall. Hence, future research should consider such aspects and focus on richer learning contexts.

Originality/value

This work offers practical recommendations for instructional design strategies aiming to create multisensory stimulations with VR and olfactory components to foster a richer learning experience and enhanced learning outcomes, under the assumptions of a stereotypical learning context.
Internalizing and externalizing symptoms in children during the COVID-19 pandemic: a systematic mixed studies review
Authors: Levante Annalisa , Martis Chiara , Bianco Federica , Castelli Ilaria , Petrocchi Serena , Lecciso Flavia
Published: June 2023

Introduction

Given the vulnerability of children during the COVID-19 pandemic, paying close attention to their wellbeing at the time is warranted. The present protocol-based systematic mixed-studies review examines papers published during 2020–2022, focusing on the impact of the COVID-19 pandemic on children's internalizing/externalizing symptoms and the determinants thereof.

Method

PROSPERO: CRD42022385284. Five databases were searched and the PRISMA diagram was applied. The inclusion criteria were: papers published in English in peer-reviewed journals; papers published between January 2020 and October 2022 involving children aged 5–13 years; qualitative, quantitative, and mixed studies. The standardized Mixed Method Appraisal Tool protocol was used to appraise the quality of the studies.

Results

Thirty-four studies involving 40,976 participants in total were analyzed. Their principal characteristics were tabulated. The results showed that children's internalizing/externalizing symptoms increased during the pandemic, largely as a result of disengagement from play activities and excessive use of the internet. Girls showed more internalizing symptoms and boys more externalizing symptoms. Distress was the strongest parental factor mediating children's internalizing/externalizing symptoms. The quality of the studies was appraised as low (n= 12), medium (n= 12), and high (n= 10).

Conclusion

Gender-based interventions should be designed for children and parents. The studies reviewed were cross-sectional, so long-term patterns and outcomes could not be predicted. Future researchers might consider a longitudinal approach to determine the long-term effects of the pandemic on children's internalizing and externalizing symptoms.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022385284, identifier: CRD42022385284.
Psychometric properties and invariance of an English self-efficacy scale for university students in Peru
Authors: Mendoza-Torres Mariela Estela , Morales-García Wilter C. , Sairitupa-Sanchez Liset Z. , Morales-García Sandra B. , Rivera-Lozada Oriana , Sucapuca-Sucapuca Francisco E. , Cunza-Aranzábal Denis Frank
Published: June 2023

Background

English is a global language used to communicate with members of the international community. Self-efficacy in learning English is an important factor that is influenced by perceived importance, interest, and beliefs about the ability to successfully perform tasks in English.

Objective

The aim is to develop and validate a measurement instrument to assess English self-efficacy.

Methods

A total of 453 students from different Peruvian universities participated, with ages ranging from 18 to 60 years (M = 23; SD = 6.18). Statistical techniques for latent variables were used and recommendations for educational and psychological tests were followed in its construction. The sample was divided into two groups to perform exploratory factor analysis (EFA) and confirmatory factor analysis (CFA).

Results

The English Self-Efficacy Scale (ESS-P) is representative and relevant in terms of its item content (Aiken’s V > 0.70). Its internal structure is organized into three first order factors and a second order factor that are consistent with the theoretical proposal and was confirmed through CFA with excellent goodness-of-fit indices (χ2 = 1184.9, gl = 626, CFI = 0.92, TLI = 0.92, RMSEA = 0.06 y SRMR = 0.04). It also has adequate internal consistency in its three factors (Reading α/ω = 0.96), Oral Communication (α/ω = 0.95), and Writing (α/ω = 0.97) and the whole scale (α/ω = 0.98), is invariant with respect to sex, and has a conceptual relationship with variables such as academic self-efficacy and exam anxiety.

Conclusion

The ESS-P is a measurement instrument with evidence of validity, factorial invariance, and good reliability of its scores. Therefore, it can be used in future studies in the academic context.
Bridge connection between depression and anxiety symptoms and lifestyles in Chinese residents from a network perspective
Authors: Wang Shi-Bin , Xu Wen-Qi , Gao Li-Juan , Tan Wen-Yan , Zheng Hui-Rong , Hou Cai-Lan , Jia Fu-Jun
Published: June 2023

Background

Lifestyle habits are vital components of the culture of mental health treatment settings. We examined the bridge connection between depressive and anxiety symptoms and lifestyles from a network perspective using a population-based study.

Methods

Face-to-face interviews were conducted with a provincially representative sample of 13,768 inhabitants from the Guangdong Sleep and Psychosomatic Health Survey based on standardized evaluation techniques. We identified the central symptoms by expected influence. The interconnection between depression and anxiety symptoms, as well as the bridge connectivity linking depression–anxiety symptoms and lifestyle factors, were assessed using the bridge centrality index. Network stability and sensibility analyses were performed using a case-dropping bootstrap procedure.

Results

The core symptom that exhibited the highest expected influence was fatigue or little energy, followed by uncontrollable worry, trouble relaxing, and sad mood in the depression-anxiety symptoms network, while guilt was the most interconnected symptom and had the highest bridge strength. Surrounding nodes of each node explained an average variance of 57.63%. Additionally, suicidal thoughts were recognized as collective bridging symptoms connecting lifestyle variables in the network integrating depression-anxiety symptoms with lifestyle factors. Current tobacco and alcohol consumption were positively associated with suicidal thoughts and irritability. Habitual diet rhythm and physical exercise frequency were linked to suicidal thoughts, guilt, and poor appetite or overeating. Suicidal thoughts, irritability, and guilt indicated the greatest connectivity with lifestyle factors. All networks had high stability and accuracy.

Conclusion

These highlighted core and bridge symptoms could serve as latent targets for the prevention and intervention of comorbid depression and anxiety. It might be crucial for clinical practitioners to design effective and targeted treatment and prevention strategies aiming at specific lifestyles and behaviors.
Metagenomic-based pathogen surveillance for children with severe pneumonia in pediatric intensive care unit
Authors: Li Meijin , Wang Jing , Yao Zhongwei , Liao Hailing , Su Shufen , Yang Xuying , Xie Mingzhou , Zheng Yinan
Published: June 2023

Background

Pneumonia is a significant cause of morbidity and mortality in children. Metagenomic next-generation sequencing (mNGS) has the potential to assess the landscape of pathogens responsible for severe pulmonary infection.

Methods

Bronchoalveolar lavage fluid (BALF) samples of 262 children with suspected pulmonary infections were collected from April 2019 to October 2021 in the Pediatric Intensive Care Unit (PICU) of Guangdong Women and Children Hospital. Both mNGS and conventional tests were utilized for pathogen detection.

Results

A total of 80 underlying pathogens were identified using both mNGS and conventional tests. Respiratory syncytial virus (RSV), Staphylococcus aureus and rhinovirus were the most frequently detected pathogens in this cohort. The incidence rate of co-infection was high (58.96%, 148/251), with bacterial-viral agents most co-detected. RSV was the main pathogen in children younger than 6 months of age, and was also commonly found in older pediatric patients. Rhinovirus was prevalent in children older than 6 months. Adenovirus and Mycoplasma pneumoniae were more prevalent in children older than 3 years than in other age groups. Pneumocystis jirovecii was detected in nearly 15% of children younger than 6 months. Besides, influenza virus and adenovirus were rarely found in 2020 and 2021.

Conclusions

Our study highlights the importance of using advanced diagnostic techniques like mNGS to improve our understanding of the microbial epidemiology of severe pneumonia in pediatric patients.
Antimicrobial susceptibility testing and tentative epidemiological cut-off values for Lactobacillaceae family species intended for ingestion
Authors: Nøhr-Meldgaard Katrine , Struve Carsten , Ingmer Hanne , Koza Anna , Al-Nakeeb Kosai , Agersø Yvonne
Published: June 2023

Introduction

In this work, 170 strains covering 13 species from the Lactobacillaceae family were analyzed to determine minimal inhibitory concentration (MIC) distributions to nine antimicrobial agents, and genes potentially conferring resistance. This allows a proposal of tentative Epidemiological Cut-Offs (ECOFFs) that follows the phylogeny for interpretation of resistance in the 13 species.

Methods

The 170 strains originated from different sources, geographical areas, and time periods. MICs for nine antibiotics were determined according to the ISO 10932 standard for lactobacillia and by a modified CLSI-method for Leuconostoc and Pediococcus which ensured sufficient growth. The strains were whole genome sequenced, subtyped by core genome analysis, and assessed for the presence of antibiotic resistance genes using the ResFinder and NCBI AMRFinder databases.

Results and discussion

The data provide evidence that antimicrobial susceptibility follows phylogeny instead of fermentation pattern and accordingly, tentative ECOFFs were defined. For some species the tentative ECOFFs for specific antibiotics are above the cut-off values set by the European Food Safety Authority (EFSA) which are primarily defined according to fermentation pattern or at genus level. The increased tolerance for specific antibiotics observed for some species was evaluated to be innate, as only for one strain phenotypic resistance was found to be related to an acquired resistance gene. In general, more data are needed to define ECOFFs and since the number of isolates available for industrial relevant bacterial species are often limited compared to clinically relevant species, it is important; 1) that strains are unambiguously defined at species level and subtyped through core genome analysis, 2) MIC determination are performed by use of a standardized method to define species-specific MIC distributions and 3) that known antimicrobial resistance genes are determined in whole genome sequences to support the MIC determinations.
Unobtrusive cot side sleep stage classification in preterm infants using ultra-wideband radar
Authors: Arasteh Emad , de Groot Eline R. , van den Ende Demi , Alderliesten Thomas , Long Xi , de Goederen Robbin , Benders Manon , Dudink Jeroen
Published: June 2023

Background

Sleep is an important driver of development in infants born preterm. However, continuous unobtrusive sleep monitoring of infants in the neonatal intensive care unit (NICU) is challenging.

Objective

To assess the feasibility of ultra-wideband (UWB) radar for sleep stage classification in preterm infants admitted to the NICU.

Methods

Active and quiet sleep were visually assessed using video recordings in 10 preterm infants (recorded between 29 and 34 weeks of postmenstrual age) admitted to the NICU. UWB radar recorded all infant's motions during the video recordings. From the baseband data measured with the UWB radar, a total of 48 features were calculated. All features were related to body and breathing movements. Six machine learning classifiers were compared regarding their ability to reliably classify active and quiet sleep using these raw signals.

Results

The adaptive boosting (AdaBoost) classifier achieved the highest balanced accuracy (81%) over a 10-fold cross-validation, with an area under the curve of receiver operating characteristics (AUC-ROC) of 0.82.

Conclusions

The UWB radar data, using the AdaBoost classifier, is a promising method for non-obtrusive sleep stage assessment in very preterm infants admitted to the NICU.
Protest tourism as gendered experience: constraints, feelings and gender roles of female activists
Authors: Tominaga Kyoko
Published: June 2023

This paper investigates protest tourism as a gendered experience. Recently, scholars have shown increasing interest in protest tourism, but they have mainly focused on the participants' motivation, emotions, and identity. Understanding gender differences in protest tourism is important in the investigation of both protests and tourism. This study discusses Japanese female protest tourism, focusing on three aspects: constraints, feelings and gender roles. Previous studies on tourism and social movements provide a research framework for these three aspects. Based on writings described by female activists and on interviews with participants, the paper reports three key findings. First, female participants face constraints when taking part in protest tourism due to domestic responsibilities and resource limitations. Second, women's protest tourists have fears about the risks of harassment and violence and are uncomfortable being in a marginalized position. Third, they are forced into a feminine role in demonstration speeches and accommodations; however, if they ignore social norms, they can liberate themselves from that role. These findings demonstrate the discouragement factors associated with protest tourism, namely its danger, one-time nature and uniqueness, by focusing on the marginalized actor. The discussion of the concept of protest tourism therefore includes a wider range of behaviors.
Enhancement of storage life and maintenance of quality in tomato fruits by preharvest salicylic acid treatment
Authors: Baninaiem Elham , Dastjerdi Abdolmajid Mirzaalian
Published: June 2023

Tomato fruits are usually characterized by a short postharvest life. Their quality decreases during storage due to losses in nutritional quality and decay. Salicylic acid (SA) has an important role in plant development and response to environmental stress. This research aimed at evaluating the effects of preharvest salicylic acid application on extending the storage life, and ripening of tomatoes (Solanum lycopersicum cv. Baraka). As a hybrid seed, the “Baraka” cultivar is appropriate for subtropical regions. Three consecutive weeks before harvest, foliar spraying with SA was applied at different concentrations (1, 2, and 4 mM). After storage for 10, 20, 30, and 40 days at 10°C with 85–95% relative humidity (RH), the tomatoes had decreased levels of a* value (redness), decay, electrolyte leakage, and chilling injury after being treated with salicylic acid. In comparison to the control, these characteristics were linked to increased levels of firmness, titratable acidity, ascorbic acid, and ascorbate peroxidase activity. According to the findings, fruit quality was best maintained at 4 mM SA, whereas it was least effective at 1 mM SA. Overall, the results showed that, SA can be considered a potent treatment that can effectively control postharvest loss.
Winning the race with aging: age-related changes in gait speed and its association with cognitive performance in dogs
Authors: Mondino Alejandra , Khan Michael , Case Beth , Fefer Gilad , Panek Wojciech K. , Gruen Margaret E. , Olby Natasha J.
Published: June 2023

Introduction

In humans, gait speed is a crucial component in geriatric evaluation since decreasing speed can be a harbinger of cognitive decline and dementia. Aging companion dogs can suffer from age-related mobility impairment, cognitive decline and dementia known as canine cognitive dysfunction syndrome. We hypothesized that there would be an association between gait speed and cognition in aging dogs.

Methods

We measured gait speed on and off leash in 46 adult and 49 senior dogs. Cognitive performance in senior dogs was assessed by means of the Canine Dementia Scale and a battery of cognitive tests.

Results

We demonstrated that dogs' food-motivated gait speed off leash is correlated with fractional lifespan and cognitive performance in dogs, particularly in the domains of attention and working memory.

Discussion

Food-motivated gait speed off leash represents a relatively easy variable to measure in clinical settings. Moreover, it proves to be a more effective indicator of age-related deterioration and cognitive decline than gait speed on leash.
Advancing the 3Rs: innovation, implementation, ethics and society
Authors: Grimm Herwig , Biller-Andorno Nikola , Buch Thorsten , Dahlhoff Maik , Davies Gail , Cederroth Christopher R. , Maissen Otto , Lukas Wilma , Passini Elisa , Törnqvist Elin , Olsson I. Anna S. , Sandström Jenny
Published: June 2023

The 3Rs principle of replacing, reducing and refining the use of animals in science has been gaining widespread support in the international research community and appears in transnational legislation such as the European Directive 2010/63/EU, a number of national legislative frameworks like in Switzerland and the UK, and other rules and guidance in place in countries around the world. At the same time, progress in technical and biomedical research, along with the changing status of animals in many societies, challenges the view of the 3Rs principle as a sufficient and effective approach to the moral challenges set by animal use in research. Given this growing awareness of our moral responsibilities to animals, the aim of this paper is to address the question: Can the 3Rs, as a policy instrument for science and research, still guide the morally acceptable use of animals for scientific purposes, and if so, how? The fact that the increased availability of alternatives to animal models has not correlated inversely with a decrease in the number of animals used in research has led to public and political calls for more radical action. However, a focus on the simple measure of total animal numbers distracts from the need for a more nuanced understanding of how the 3Rs principle can have a genuine influence as a guiding instrument in research and testing. Hence, we focus on three core dimensions of the 3Rs in contemporary research: (1) What scientificinnovationsare needed to advance the goals of the 3Rs? (2) What can be done to facilitate theimplementationof existing and new 3R methods? (3) Do the 3Rs still offer an adequate ethical framework given the increasingsocial awarenessof animal needs and human moral responsibilities? By answering these questions, we will identify core perspectives in the debate over the advancement of the 3Rs.
Case report: Laryngospasm following ethanol ablation of a parathyroid nodule in a dog with primary hyperparathyroidism
Authors: Rank Kaitlyn , Lynch Alex M. , Green Randolph , Reed-Jones Leslie , Harrell Karyn , Ueda Yu
Published: June 2023

A 12-year-old female spayed dachshund was presented for emergency assessment of respiratory distress, characterized by inspiratory dyspnea with stridor. Percutaneous ultrasound-guided ethanol ablation of a functional parathyroid tumor was performed 72-h earlier for management of primary hyperparathyroidism. The dog was hypocalcemic (ionized calcium 0.7 mmol/L, reference interval: 0.9–1.3 mmol/L) at the time of presentation and had evidence of laryngospasm on a sedated oral exam. The dog was managed conservatively with supplemental oxygen, anxiolysis, and parenteral calcium administration. These interventions were associated with rapid and sustained improvement in clinical signs. The dog did not demonstrate any recurrence of signs afterwards. To the authors' knowledge, this is the first description of laryngospasm following ethanol ablation of a parathyroid nodule in a dog that developed hypocalcemia.
Management of Digital Dermatitis in Dairy Herds: Optimization and Time Allocation
Authors: Robcis Rodolphe , Ferchiou Ahmed , Berrada Mehdi , Raboisson Didier
Published: June 2023

The objective of this study is to determine the most effective time allocation for digital dermatitis management and to quantify the marginal economic gain from extra time devoted to digital dermatitis management. The model simulating foot disease occurrence and the associated management was Dairy Health Simulator©. Then, an econometric model was applied to identify the relationship between the dairy workshop’s gross margin and time for lameness management as well as the hourly marginal gain curve associated with lameness management. The gross margin was optimized under two constraints, i.e., the overall time spent for lameness management and the mean lameness duration (mimicking cow welfare). The results show that allocating less time to lameness detection and more to footbath application can contribute as follows: (i) reduces time spent for lameness management, (ii) maintains the best welfare level, and (iii) obtains the highest gross margin. The optimal time to devote to footbath application ranged from 17.8 to 22.3 h/month. A hiring strategy was investigated, and the break-even point ranged from 16.1 to 19.8 h/month. The recommended time to spend on footbath application is relatively important; therefore, stakeholders should consider the importance of lameness to the dairy industry and should devote enough time for footbath applications.
Pre-Slaughter Stunning of Farmed Atlantic Halibut in CO2-Saturated Seawater: Assessment of Unconsciousness by Electroencephalography (EEG)
Authors: Rucinque Daniel Santiago , van de Vis Hans , Reimert Henny , Roth Bjørn , Foss Atle , Taconeli Cesar Augusto , Gerritzen Marien
Published: June 2023

As fish welfare becomes a growing concern, it is important to ensure humane treatment during slaughter. This study aimed to assess the onset of unconsciousness in Atlantic halibut immersed in CO2-saturated seawater through electroencephalography (EEG). Of the 29 fish studied, 10 exhibited escape attempts, indicating aversion to CO2-saturated water despite its oxygenation. EEG signals showed four distinct phases: transitional, excitation (high amplitude–high frequency), suppressed, and iso-electric phases. The onset of the suppressed phase, indicative of unconsciousness, occurred on average 258.8 ± 46.2 s after immersion. The spectral analysis of the EEG signals showed a progressive decrease in median frequency, spectral edge frequency, and high frequency contribution, which corresponded to the gradual loss of consciousness. The study concludes that CO2-saturated water is not recommended for pre-slaughter handling of halibut due to the extended time required for the onset of unconsciousness and the observed aversive behaviour. Ensuring humane treatment during slaughter is important for addressing public concern and safeguarding fish welfare in all stages of production.
Optimal Sensor Deployment for Parameter Estimation Precision by Integrating Bayesian Networks in Wet-Grinding Systems
Authors: He Kang , Wu Bo , Sun Fei , Yang Quan , Yang Huichao
Published: June 2023

Accurately and efficiently determining a system’s physical variables is crucial for precise product-quality control. This study proposes a novel method for optimal sensor deployment to increase the accuracy of sensing data for physical variables and ensure the timely detection of the product’s particle size in a wet-grinding system. This approach involves three steps. First, a Bayesian network (BN) is designed to model the cause–effect relationship between the physical variables by applying the path model. The detectability is determined to confirm that the mean shifts of all the physical variables are identifiable using sensor sets in the wet-grinding system. Second, the sensing location of accelerometers mounted on the chamber shell is determined according to the coupled computational fluid dynamics–discrete element method simulations. Third, the shuffled frog leaping algorithm is developed by combining the BN to minimize the maximum data output deviation index among all sensor sets and sensory costs; this is achieved under the constraints of the mean shift detectability, achieving optimum sensor allocation. Subsequently, a case study is performed on a zirconia powder production process to demonstrate that the proposed approach minimizes the requirements of the data output deviation index, sensory costs, and detectability. The proposed approach is systematic and universal; it can be integrated into monitor architecture for parameter estimation in other complex production systems.
Readability across Time and Languages: The Case of Matthew’s Gospel Translations
Authors: Matricciani Emilio
Published: June 2023

We have studied how the readability of a text can change in translation by considering Matthew’s Gospel, written in Greek, translated into Latin and 35 modern languages. We have found that the deep-language parameters CP (characters per word), PF (words per sentence), IP (words per interpunctions), MF (interpunctions per sentence) and a universal readability index GU  of each translation are so diverse from language to language, and even within a given language for which there are many versions of Matthew—such as in English and Spanish—that the resulting texts mathematically seem to be diverse. The several tens of versions of Matthew’s Gospel studied appear to address very diverse audiences. If a reader could understand all of them well, he/she would have the impression of reading texts written by diverse authors, although all of them tell the same story.
Research on the Evaluation of Air Quality in Underground Coal Mines Based on a Generalized Contrastive Weighted Comprehensive Scale Index Method
Authors: Li Shihang , Chen Xingyue , Peng Gangcheng , Han Muze , Guo Qiaosong , Hou Jun , Gao Bohan
Published: June 2023

In this study, an optimization model was established based on the generalized contrastive weighted comprehensive scale index method. This model gives the evaluation indicators of SO2, NOx, CO, and TSP. It also innovatively introduces gas, the most harmful substance in underground coal mines, into the evaluation indicators. Moreover, the obvious hazardous concentration limit is used as the third standard concentration of the model. The scale sub-indices and the weights of SO2, NOx, CO, TSP, and gas are calculated, leading to the comprehensive scale index. Finally, the classification standard of the underground air quality is determined. An underground excavation face in Shaanxi Province is used as an example for air quality assessment. The air quality is generally poor at the points close to the working face, while that at the points far away from the working face is generally better. Furthermore, air quality optimization measures are given for areas with poor air quality.
Attenuation Correction of the X-Band Dual-Polarization Phased Array Radar Based on Observed Raindrop Size Distribution Characteristics
Authors: Feng Jiabao , Liu Xiantong , Xia Feng , Zhang Yu , Rao Xiaona
Published: June 2023

X-band dual-polarization phased array radar (XPAR-D) possesses high resolution and plays a significant role in detecting meso- and micro-scale convective systems. However, the precipitation attenuation it endures necessitates an effective correction method. This study selected radar data from XPAR-D at the peak of Maofeng Mountain in Guangzhou during 16–17 May 2020 from three precipitation stages after quality control. Attenuation coefficients were calculated for different precipitation types through scattering simulations of raindrop size distribution (RSD) data. Next, an attenuation correction algorithm (MZH-KDP method) was proposed for the radar reflectivity factor (ZH) according to different raindrop types and compared to the ZH-KDP method currently in use. The results indicate that the attenuation amount of XPAR-D echoes depends on the attenuation path and echo intensity. When the attenuation path is shorter and the echo intensity is weaker, the amount of attenuation and correction is smaller. Difficulties arise when there are noticeable deviations, which are challenging to resolve using attenuation correction methods. Longer attenuation paths and stronger echoes highlight the advantages of the MZH-KDP method, while the ZH-KDP method tends to overcorrect the bias. The MZH-KDP method outperforms the ZH-KDP method for different precipitation types. The superior correction capability of the MZH-KDP method provides a significant advantage in improving the performance of XPAR-D for the detection of extreme weather.
Sea Ice Extent Prediction with Machine Learning Methods and Subregional Analysis in the Arctic
Authors: Chen Siwen , Li Kehan , Fu Hongpeng , Wu Ying Cheng , Huang Yiyi
Published: June 2023

The decline of sea ice in the Arctic region is a critical indicator of rapid global warming and can also influence the feedback processes in the Arctic, so the prediction of sea ice extent and thickness plays an important role in climate modeling and prediction. This paper uses machine learning methods to predict the sea ice extent, and by adjusting the methods and factors, which include the climate variables, the past sea ice extent, and the simple linear-regression-simulated sea ice extent, then we found the best combination to give the result with the highest R2 score. We noticed that with longer periods of past sea ice extent data and shorter periods of climate data, the results appeared to be better. This might be related to the difference in climate and ocean memory. The sub-region sea ice extent prediction shows that the regions with whole-year ice cover are easier to predict and that those regions with sudden weather changes and significant seasonal variability appear to have lower R2 scores in the sea ice extent prediction.
Reduced Resting-State EEG Power Spectra and Functional Connectivity after 24 and 36 Hours of Sleep Deprivation
Authors: Lian Jie , Xu Lin , Song Tao , Peng Ziyi , Zhang Zheyuan , An Xin , Chen Shufang , Zhong Xiao , Shao Yongcong
Published: June 2023

Total sleep deprivation (TSD) leads to cognitive decline; however, the neurophysiological mechanisms underlying resting-state electroencephalogram (EEG) changes after TSD remain unclear. In this study, 42 healthy adult participants were subjected to 36 h of sleep deprivation (36 h TSD), and resting-state EEG data were recorded at baseline, after 24 h of sleep deprivation (24 h TSD), and after 36 h TSD. The analysis of resting-state EEG at baseline, after 24 h TSD, and after 36 h TSD using source localization analysis, power spectrum analysis, and functional connectivity analysis revealed a decrease in alpha-band power and a significant increase in delta-band power after TSD and impaired functional connectivity in the default mode network, precuneus, and inferior parietal lobule. The cortical activities of the precuneus, inferior parietal lobule, and superior parietal lobule were significantly reduced, but no difference was found between the 24 h and 36 h TSD groups. This may indicate that TSD caused some damage to the participants, but this damage temporarily slowed during the 24 h to 36 h TSD period.
Effects of Twins Therapy on Egocentric and Allocentric Neglect in Stroke Patients: A Feasibility Study
Authors: Jang Woo-Hyuk , Hwang Hyeong-Min , Kim Jae-Yeop
Published: June 2023

(1) Background: Existing treatment methods for neglect are concentrated on egocentric neglect and may lead to various problems such as cost/space constraints and portability. Therefore, this study seeks to determine how a new treatment (also known as twins therapy, TT) for stroke patients can improve an existing problem associated with neglect. (2) Method: A pre/post-test control group research design was used and both groups continued to receive existing rehabilitation treatment, whilst TT intervention was only added to the experimental group. TT intervention was conducted for a total of 20 sessions (1 session for 30 min/day, 5 days/week, for 4 weeks). (3) Result: There was no significant difference in the manual function test (MFT) and the Korean version of the Modified Barthel Index (K-MBI) items (p > 0.05) before and after the TT intervention. However, the score and execution time of the apple cancellation test showed a significant reduction only in the experimental group (p < 0.05). (4) Conclusion: TT not only improved egocentric neglect, but also allocentric neglect symptoms in stroke patients.