bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study of Milk Composition and Coagulation Properties of Holstein-Friesian, Jersey, and Their Cross Milked Once or Twice a Day
Authors: Sanjayaranj Inthujaa , Lopez-Villalobos Nicolas , Blair Hugh T. , Janssen Patrick W. M. , Holroyd Stephen E. , MacGibbon Alastair K. H.
Published: February 2023

The objective of the study was to explore the effect of breed on the composition and coagulation properties (rennet coagulation time (min), curd firming rate (min), and curd firmness (mm)) of milk from cows milked once a day or twice a day in the morning and afternoon, using a Formagraph. Thirty cows (11 Holstein-Friesian, 8 Holstein-Friesian × Jersey cross, and 11 Jersey) from a once-a-day milking herd and thirty cows (16 Holstein-Friesian, 10 Holstein-Friesian × Jersey cross, and 4 Jersey) from a twice-a-day milking herd were sampled in late lactation. The milk composition and coagulation properties were analysed for each milk sample. Jersey cows had better milk coagulation properties at each milking frequency-milking time compared to Holstein-Friesian cows. Curd firmness 30 min after the addition of rennet was positively (p < 0.05) correlated with the protein concentration. However, the correlations were inconsistent between milking frequencies and milking times, resulting in poor prediction of the changes in cheese-making potential. This study indicated that milk composition and coagulation properties were affected by breed and milking frequency. The effect of the breed could be due to the variation in the composition of the milk, but firm recommendations were hampered by a low number of samples. Further research with larger cow numbers is justified.
Rapid Melanoma Death of an Adult Male with Congenital Bathing Trunk Nevus despite Initiation of Combination Immunotherapy
Authors: Gambichler Thilo , Noldes Kathrin , Arafat Yousef , Neid Matthias , Rütten Arno , Boms Stefanie
Published: February 2023

Dear Editors: Giant congenital melanocytic naevus (GCMN)-associated melanoma in adults is very rare [...]
Protein Misfolding and Aggregation in Proteinopathies: Causes, Mechanism and Cellular Response
Authors: Ajmal Mohammad Rehan
Published: February 2023

Proteins are central to life functions. Alterations in the structure of proteins are reflected in their function. Misfolded proteins and their aggregates present a significant risk to the cell. Cells have a diverse but integrated network of protection mechanisms. Streams of misfolded proteins that cells are continuously exposed to must be continually monitored by an elaborated network of molecular chaperones and protein degradation factors to control and contain protein misfolding problems. Aggregation inhibition properties of small molecules such as polyphenols are important as they possess other beneficial properties such as antioxidative, anti-inflammatory, and pro-autophagic properties and help neuroprotection. A candidate with such desired features is important for any possible treatment development for protein aggregation diseases. There is a need to study the protein misfolding phenomenon so that we can treat some of the worst kinds of human ailments related to protein misfolding and aggregation.
Thermodynamics of an Empty Box
Authors: Schmitz Georg J. , te Vrugt Michael , Haug-Warberg Tore , Ellingsen Lodin , Needham Paul , Wittkowski Raphael
Published: February 2023

A gas in a box is perhaps the most important model system studied in thermodynamics and statistical mechanics. Usually, studies focus on the gas, whereas the box merely serves as an idealized confinement. The present article focuses on the box as the central object and develops a thermodynamic theory by treating the geometric degrees of freedom of the box as the degrees of freedom of a thermodynamic system. Applying standard mathematical methods to the thermodynamics of an empty box allows equations with the same structure as those of cosmology and classical and quantum mechanics to be derived. The simple model system of an empty box is shown to have interesting connections to classical mechanics, special relativity, and quantum field theory.
Research on High-Torque-Density Design for Axial Modular Flux-Reversal Permanent Magnet Machine
Authors: Lin Shan , Chang Lanchao , Su Peng , Li Yongjian , Hua Wei , Shen Yi
Published: February 2023

The traditional flux reversal permanent magnet (FRPM) machine has high torque ripple due to the double salient-pole structure, and the effective air-gap length is increased by the permanent magnet structure of the stator tooth surface, which affects the size of the air-gap magnetomotive force (MMF). This paper proposes an axial modular flux-reversal permanent magnet (AM-FRPM) machine with attractive torque capabilities. Based on air-gap magnetic field modulation theory, a method to achieve optimal air-gap harmonic torque contributions was developed. Then, the principle for high-torque-density generation in the AM-FRPM machine under an alternating magnetization topology was investigated using the PM magnetic field modulation and armature reaction magnetic field modulation. In addition, the cogging torque suppression mechanism, which guides the selection of stator-slot and rotor-pole combinations, was investigated. In addition, a comprehensive comparison of the electromagnetic characteristics of two AM-FRPM machines and a traditional FRPM machine was conducted. Then, the advantages and disadvantages of the three machines were analyzed. Finally, prototypes were manufactured and tested to verify the correctness of the theoretical analysis.
Concerns on Full Electric Mobility and Future Electricity Demand in Italy
Authors: Falfari Stefania , Bianchi Gian
Published: February 2023

This work aims to provide an energy and critical analysis of the new “ecological scenario” that sees the world shift the transport sector from the classic fossil-fueled traction towards a purely electric traction. Is it a transition to a cleaner and more environmentally friendly world? This is the central question we need to answer. In this context of forced electrification of passenger vehicles, the authors estimated the electricity surplus needed in Italy to face this transition with a projection up to 2050, considering the electricity demand of purely electric vehicles and for the production of green hydrogen for the fuel supply of fuel cell vehicles. Throughout this investigation, the authors discovered that, in 2050, the surplus of electricity to be produced compared to the current production in Italy (year 2021) is equal to +27.6% (1). This value increases if two limit scenarios are considered: it becomes +40.0% within a limit scenario in which the entire private car fleet is made up of pure electric traction vehicles only (2). It becomes +100.6% in the hypothesis that the entire fleet of passenger cars is made up of fuel cell vehicles powered exclusively by green hydrogen (3).
Economic Analysis of a Pumped Hydroelectric Storage-Integrated Floating PV System in the Day-Ahead Iberian Electricity Market
Authors: Barbón Arsenio , Gutiérrez Ángel , Bayón Luis , Bayón-Cueli Covadonga , Aparicio-Bermejo Javier
Published: February 2023

This study identifies the optimal operational strategy for floating photovoltaic power plants and pumped hydroelectric power plants in the day-ahead Iberian electricity market. Different operating scenarios were analysed based on forecast accuracy in addition to any deviations occurring in the day-ahead market, taking into account the rules of the electricity market and the technical operational limitations of both plants. These scenarios show the choice between the independent mode of operation and the joint mode of operation of both plants. Five scenarios have been studied, with upward and downward deviations of 5%, 10%, 25% and 50% considered. These scenarios can be classified into two groups. If there are deviation penalties, group 1; or without deviation penalties, group 2. Scenarios 3 and 4 belong to the first group and scenarios 1, 2 and 5 to the second group. In the scenarios of the first group, the price deviations are used, and in the scenarios of the second group, the marginal market price is used. The economic benefit of the scenarios with deviation penalties is obtained in the joint operation mode of both plants. Economic benefits of up to 35% are obtained. In contrast, in the scenarios where there are no deviation penalties, the independent mode of operation is the optimum. The reason for this is the low efficiency of the pumping process. In this case, economic benefits of 1.6% are obtained. This study can be used to guide the decision-making process in the operation of both plants in order to maximise the economic benefit.
Forecasting Electricity Demand by Neural Networks and Definition of Inputs by Multi-Criteria Analysis
Authors: Deina Carolina , dos Santos João Lucas Ferreira , Biuk Lucas Henrique , Lizot Mauro , Converti Attilio , Siqueira Hugo Valadares , Trojan Flavio
Published: February 2023

The planning of efficient policies based on forecasting electricity demand is essential to guarantee the continuity of energy supply for consumers. Some techniques for forecasting electricity demand have used specific procedures to define input variables, which can be particular to each case study. However, the definition of independent and casual variables is still an issue to be explored. There is a lack of models that could help the selection of independent variables, based on correlate criteria and level of importance integrated with artificial networks, which could directly impact the forecasting quality. This work presents a model that integrates a multi-criteria approach which provides the selection of relevant independent variables and artificial neural networks to forecast the electricity demand in countries. It provides to consider the particularities of each application. To demonstrate the applicability of the model a time series of electricity consumption from a southern region of Brazil was used. The dependent inputs used by the neural networks were selected using a traditional method called Wrapper. As a result of this application, with the multi-criteria ELECTRE I method was possible to recognize temperature and average evaporation as explanatory variables. When the variables selected by the multi-criteria approach were included in the predictive models, were observed more consistent results together with artificial neural networks, better than the traditional linear models. The Radial Basis Function Networks and Extreme Learning Machines stood out as potential techniques to be used integrated with a multi-criteria method to better perform the forecasting.
Application of the Temperature Oscillation Method to Laminar Flow in Straight Horizontal and Curved Minichannels
Authors: Solnař Stanislav , Haase Stefan , Jirout Tomáš
Published: February 2023

This work deals with the application of the temperature oscillation method to measure local values of the heat transfer coefficient in laminar flow in a straight horizontal pipe and in a pipe with a bend. This method, applied here for the first time in such experimental conditions, uses a time-varying heat flux as a boundary condition, which is a condition of the third kind. Since an analytical solution for such conditions could not be identified in the existing literature, a numerical study of this problem is applied here. Experimental data for a straight horizontal pipe confirm the numerical results within a reasonable level of error. Experimental measurements on a straight horizontal tube are consistent with predicted results in the literature for both total and local heat transfer coefficient values. The measurements on bent tubes show a very significant influence on the local values compared to the straight tube and in the overall values this change appears as an increase in the heat transfer coefficient.
Influence of Environmental Factors on the Intelligent Management of Photovoltaic and Wind Sections in a Hybrid Power Plant
Authors: Kusznier Jacek
Published: February 2023

The high-efficiency operation of photovoltaic and wind systems is affected by many factors and parameters that should be continuously monitored. Since most of the variable factors are related to weather conditions, they are difficult to predict. Therefore, in order to optimize the operating point of a photovoltaic or wind power plant, it is necessary to observe changes in the subject area. The operation of photovoltaic and wind power plants can complement each other. The results recorded at the hybrid power plant of the Faculty of Electrical Engineering of Bialystok University of Technology are useful for a comprehensive analysis of the power plant operation and the ways to optimize it. This paper presents the influence of environmental factors on the operation of a hybrid photo-voltaic–wind power plant located in the city of Bialystok, Poland. The aim of the study was to present the variable factors on the optimal adjustment of the location of the power plant elements at the stage of its design and selection of the energy management system. The presented measurement data from 2015–2021 allow conclusions to be drawn on the significant impact on the power plant’s operation, taking into account both the average conditions corresponding to the analysed location and the full range of changes in the listed factors.
17β-Estradiol Can Induce Sex Reversal in Brown Trout
Authors: Voorhees Jill M. , Mamer Elizabeth R. J. M. , Schill Daniel J. , Adams Mitchel , Martinez Carlos , Barnes Michael E.
Published: February 2023

Hormones have been used to change phenotypic sex in many fish species. However, information specific to changing sex in brown trout Salmo trutta is lacking. This study compared the effectiveness of two different 17β-estradiol (estradiol) concentrations (20 mg/kg and 30 mg/kg) fed to brown trout for 60 days, beginning at initial feeding. At 456 days post-initial feeding, the 20 mg/kg and 30 mg/kg treatment groups were 84% and 86% female, respectively. These values were significantly higher than the 47% females observed in the control group which did not receive dietary estradiol. At the end of the 60-day estradiol treatment period, weight gain, percent weight gain, and feed conversion ratio were all significantly lower in the tanks of fish receiving estradiol than in the control tanks. Individual fish fed estradiol also weighed significantly less and were significantly shorter than the fish not receiving estradiol-coated feed. Mortality ranged from 1.0 to 2.4% among the treatments and was not significantly different. After 105 days post-initial feeding, weight gain, percent weight gain, and feed conversion ratio were not significantly different among the treatments. At 456 days post-initial feeding, individual fish were significantly longer and heavier in the 20 mg/kg estradiol treatment compared to the fish in the control treatment, and the fish in the 30 mg/kg estradiol treatment were similar to the other two treatments. This study is the first to document the successful sex reversal of brown trout using estradiol. While the estradiol treatments used in this study did not lead to complete feminization, the observed 85-to-15% female-to-male phenotypic ratio indicates the successful feminization of genetic males. The levels of feminization observed in this study are suitable for this initial step to potentially develop a YY male broodstock to control invasive brown trout populations.
Averrhoa carambola L., Cyphomandra betacea, Myrciaria dubia as a Source of Bioactive Compounds of Antioxidant Properties
Authors: Nowak Dariusz , Gośliński Michał , Przygoński Krzysztof , Wojtowicz Elżbieta
Published: February 2023

Natural bioactive compounds play an important role in the prevention of various diseases. The exotic fruits Averrhoa carambola L. (star fruit), Cyphomandra betacea (tamarillo) and Myrciaria dubia (camu-camu) can be valuable sources of phytochemicals with antioxidant properties. The aim of this study has been to compare the antioxidant properties of these exotic fruits, the structure of polyphenolic compounds and the content of vitamin C and β-carotene. All the juices were analyzed for their antioxidant capacity (DPPH and ABTS assays) and the composition of phenolic compounds (TP and FBBB assays, total flavonoid content, total anthocyanins). In addition, HPLC assays were performed to analyse the content of phenolic acids, flavonoids, vitamin C and β-carotene. The results demonstrated that juice from the Myrciaria dubia fruit had the highest antioxidant capacity, which was 4.5-fold higher than that of juice from Averrhola carambola L., and nearly 7-fold higher than the antioxidant capacity of Cyphomandra betacea fruit juice. Additionally, juice from the camu-camu fruit had a 3- to 4-fold higher total polyphenol content (8290 ± 254 mg GAE L−1) and a high level of vitamin C (8410.8 ± 16.9 mg AA kg−1). In turn, tamarillo juice had a high content of total anthocyanins (5796 mg CGE L−1) and phenolic acids (mostly chlorogenic acid and caffeic acid). Juice produced from carambola had a high content of total flavonoids (1345 mg CAE L−1), and the composition of these compounds was dominated by flavanols (epicatechin). The research results justify the conclusion that fruits of Myrciaria dubia, Averrhoa carambola L., Cyphomandra betacea are rich sources of bioactive compounds with antioxidant properties, and in the near future may serve as healthful food ingredients.
Effect of Ultrasound Combinated with Sodium Hypochlorite Treatment on Microbial Inhibition and Quality of Fresh-Cut Cucumber
Authors: Zhang Chunhong , Hou Wanfu , Zhao Wenting , Zhao Shuang , Wang Pan , Zhao Xiaoyan , Wang Dan
Published: February 2023

The influence of ultrasound combined with sodium hypochlorite (US-NaClO) treatment on microorganisms and quality of fresh-cut cucumber during storage were investigated. Ultrasound (400 W, 40 kHz, US: 5, 10 and 15 min) and sodium hypochlorite (NaClO: 50, 75, 100 ppm) were used to treat fresh-cut cucumber in a single or combined treatment and stored at 4 °C for 8 days and analyzed for texture, color and flavor. The results showed that US-NaClO treatment had a synergistic effect on the inhibition of microorganisms during storage. It could significantly reduce (p < 0.05) the number of microorganisms by 1.73 to 2.17 log CFU/g. In addition, US-NaClO treatment reduced the accumulation of malondialdehyde (MDA) during storage (4.42 nmol/g) and water mobility, and maintained the integrity of the cell membrane, delayed the increase of weight loss (3.21%), reduced water loss, thus slowing down the decline of firmness (9.20%) of fresh-cut cucumber during storage. The degradation of chlorophyll (6.41%) was reduced to maintain the color of freshly cut cucumbers. At the same time, US-NaClO could maintain the content of aldehydes, the main aromatic substance of cucumber, and reduced the content of alcohols and ketones during storage. Combined with the electronic nose results, it could maintain the cucumber flavor at the end of the storage period and reduce the odor produced by microorganisms. Overall, US-NaClO was helpful to inhibit the growth of microorganisms during storage, improve the quality of fresh-cut cucumber.
Analysis of Dynamics and Diversity of Microbial Community during Production of Germinated Brown Rice
Authors: Yang Gaoji , Xu Juanjuan , Xu Yuanmei , Li Rui , Wang Shaojin
Published: February 2023

Sprouts may be contaminated with different pathogenic and spoilage microorganisms, which lead far too easily to foodborne outbreaks. The elucidations of microbial profiles in germinated brown rice (BR) are important, but the changes in the microbial composition during germination are unknown. This study aimed to investigate the microbiota composition and to monitor the dominant microbial dynamics in BR during germination using both culture-independent and -dependent methods. BR samples (HLJ2 and HN) were collected from each stage of the germination processing. The populations of microbes (total viable counts, yeast/mold counts, Bacillus cereus, and Enterobacteriaceae) of two BR cultivars increased markedly with the prolongation of the germination time. High-throughput sequencing (HTS) showed that the germination process significantly influenced the microbial composition and reduced the microbial diversity. Similar microbial communities were observed between the HLJ2 and the HN samples, but with different microbial richness. The bacterial and fungal alpha diversity achieved the maximum for ungerminated samples and declined significantly after soaking and germination. During germination, Pantoea, Bacillus, and Cronobacter were the dominant bacterial genera, but Aspergillus, Rhizopus, and Coniothyrium dominated for the fungi in the BR samples. The predominance of harmful and spoilage microorganisms in BR during germination is mainly from contaminated seeds, which highlights the potential risk of foodborne illness from sprouted BR products. The results provide new insight into the microbiome dynamics of BR and may help to establish effective decontamination measures against pathogenic microorganisms during sprout production.
Cohort Profile: The Xiamen Pubertal Growth Cohort Longitudinal Study
Authors: Wang Xijie , Li Yanhui , Gao Di , Yang Zhaogeng , Dong Bin , Dong Yanhui , Zou Zhiyong , Ma Jun
Published: February 2023

This cohort was set up to capture pubertal onset among children in China and to analyze how timing of pubertal onset would influence cardiovascular disease risk in later life. Pubertal onset was defined as secondary sexual characteristics (SSC) attained Tanner II stage, which was breast development for girls and testis of 4 mL for boys. Meanwhile, height growth spurt, defined as age of take-off and age of peak height velocity, were also involved to observe the consistency between the three indicators in discriminating pubertal onset. The study was conducted in Xiamen, China from November 2017 to November 2020 with 6-month gaps. One thousand, four hundred and sixteen children from four project schools who had not yet started puberty were involved at baseline. By November 2020, 1272 children were still under follow-up, with 945 (74.3%) of them reaching Tanner II stage. We would continue to follow the pubertal development, as well as change in crucial risk factors for cardiovascular disease in these participants. Evidence from the present cohort study would help to reveal the influence of pubertal growth on long-term cardiovascular health and would be one of the very first studies to provide such evidence from Asian countries.
The Combined Effect of the Trapezius Muscle Strengthening and Pectoralis Minor Muscle Stretching on Correcting the Rounded Shoulder Posture and Shoulder Flexion Range of Motion among Young Saudi Females: A Randomized Comparative Study
Authors: Hasan Shahnaz , Iqbal Amir , Alghadir Ahmad H. , Alonazi Asma , Alyahya Danah
Published: February 2023

Background: The shortening of the pectoralis minor muscle (PMi-M) and weakening of the lower trapezius muscle (LTr-M) affect scapular movement, resulting in the development of a rounded shoulder posture and reduction in the shoulder flexion range of motion (SFROM). Objective: This study evaluated the combined effect of LTr-M strengthening and PMi-M stretching on correcting the rounded shoulder postures and SFROM among young Saudi females. Methods: This study was based on a two-arm parallel-group repeated measures randomized comparative design. A total of sixty female participants with rounded shoulder postures were recruited and randomly allocated into groups 1 and 2 (n = 30/group). Each group performed supervised PMi-M stretching; however, group 2 performed a combination of LTr-M strengthening and PMi-M stretching. The outcomes, including rounded shoulder posture and SFROM, were assessed using the pectoralis minor length test (PMLT) and universal goniometer. A repeated measure ANOVA was used to compare the differences within-group and between-group for the outcomes measures at one-week (baseline) pre-intervention, two weeks, and three -weeks post-intervention. The significance level was set at q > 2.00 and p < 0.05 for all respective statistical analyses. Results: The within-group comparison revealed significant improvements (q > 2.00) in the outcomes of PMLT and SFROM when comparing their post-intervention scores to the baseline scores. The between-group comparison revealed a significant and an insignificant (q < 2.00) difference in the outcomes of PMLT and SFROM, respectively when comparing their scores at the second- and third-week post-intervention. Furthermore, the effect size of the intervention suggests an advantage of group 2 over group 1 in increasing the resting length of the PMi-M only among young Saudi females. Conclusions: The combined effect of LTr-M strengthening and PMi-M stretching was more beneficial than PMi-M stretching alone in correcting the rounded shoulder posture among young Saudi females by increasing PMi-M resting length. However, it could not yield a differential improvement in the SFROM outcome among them.
Factors Contributing to Leaving against Medical Advice (LAMA): A Consideration of the Patients’ Perspective
Authors: Pasay-an Eddieson , Mostoles Romeo , Villareal Sandro , Saguban Reynita
Published: February 2023

It is essential to understand why patients choose to leave the hospital in direct opposition to medical advice. This understanding could help to identify individuals who are at risk of adverse outcomes. In realizing this need, this study aimed to explore the factors contributing to the decision of patients to leave the hospital against medical advice. Materials and Methods: This research employed a descriptive-analytical approach. It was conducted in the city of Hail, Kingdom of Saudi Arabia. The participants were 13 patients who had opted to leave against medical advice from the Emergency Department of the government-subsidized hospitals. The researchers employed both purposive and snowball sampling. In snowball sampling, the researchers used referrals from initial participants to generate additional participants. Moreover, purposive sampling was used to find the individual that would best contribute to addressing the research issue. The data gathering was conducted from April to June 2022. Results: Five themes emerged based on the accounts of the 13 participant patients. These included (1) health literacy, (2) self-diagnosing, (3) unclear explanations regarding their condition, (4) prolonged waiting times, and (5) communication issues. Conclusion: The factors contributing to patients leaving against medical advice resulted in the five themes mentioned above. While interactions between patients and healthcare professionals may be challenging, pertinent health information must still be handed down clearly to patients.
Factors Associated with Tuberculosis Outcome in a Hyperendemic City in the North of Brazil
Authors: Costa Gabriel Fazzi , Garcez Juliana Conceição Dias , Marcos Weber , Ferreira Ana Lúcia da Silva , Andrade Jorge Alberto Azevedo , Rodrigues Yan Corrêa , Lima Luana Nepomuceno Gondim Costa , Conceição Emilyn Costa , Lima Karla Valéria Batista
Published: February 2023

Ananindeua city, State of Pará, North of Brazil, is a hyperendemic area for tuberculosis (TB), with a cure rate below the recommendation by the Brazilian Ministry of Health. We aimed to describe: (I) the TB incidence coefficient of Ananindeua municipality comparatively against Brazilian data; (II) TB treatment outcomes; (III) to compare the socioeconomic and epidemiological characteristics of abandonment versus cure outcome; and (IV) to evaluate the risk factors associated with TB treatment abandonment in Ananindeua city, from 2017 to 2021. This is a retrospective, descriptive, and cross-sectional epidemiological study which used secondary TB entries. Data were analyzed by linear regression, descriptive statistics, and associations were made using the Chi-square test and G-test, followed by univariate and multivariate logistic regression analyses. Cure rates ranged from 28.7% to 70.1%, abandonment between 7.3% and 11.8%, deaths from the disease ranged from 0% to 1.6%, and drug-resistant tuberculosis (TB-DR) rates had frequencies from 0% to 0.9%. Patient transfer rates to other municipalities were between 4.9% and 12.5%. The multivariate analysis showed that alcohol is almost 2 times more likely to lead an individual to abandon treatment and use of illicit drugs was almost 3 times more likely. Individuals between 20 and 59 years of age were also more likely to abandon treatment almost twice as often. Finally, data obtained in the present report is of great relevance to strengthen epidemiological surveillance and minimize possible discrepancies between the information systems and the reality of public health in high endemicity areas.
Assessment of Cervical Skeletal Trauma: The Synergistic Contribution of Forensic and Clinical Medicine to a Case of Corpse Concealment
Authors: Tarozzi Ilaria , Franceschetti Lorenzo , Bugelli Valentina
Published: February 2023

Fatal neck injuries represent a major challenge in forensic pathology because the anatomical complexity and high variability of neck structures make it often extremely difficult to differentiate true pathological findings from artifacts at autopsy. This topic becomes even more relevant when the forensic pathologist is required to make a pathophysiological evaluation of bone fractures in the absence of soft tissue to support the diagnosis. We report a case of unidentified, stone-covered, skeletonized human remains found within a pit below an abandoned building with bony lesions of the cervical spine and ribs, including a full-thickness fracture of the right lateral mass of the atlas (C1). After a careful study of the fractures was carried out by screening forensic literature and anthropological studies, clinical neurosurgical expertise was called upon to provide a reliable explanation. A rapid and violent twisting of the neck in the opposite direction from the fracture site by an attacker who pinned the victim’s torso is the scenario that most likely occurred in our case. This case report shows that the diagnosis of cervical spine injuries in skeletal remains should be the result of a multidisciplinary approach that integrates forensic, anthropological, and clinical expertise.
Damage Effect of Amorphous Carbon Black Nanoparticle Aggregates on Model Phospholipid Membranes: Surface Charge, Exposure Concentration and Time Dependence
Authors: Wang Xiao-Feng , Xu Kun , Li Xin-Rui , Liu Ya-Xin , Cheng Jie-Min
Published: February 2023

Commercial nano-scale carbon blacks (CB) are being harnessed widely and may impose potentially hazardous effects because of their unique properties, especially if they have been modified to grow reactive functional groups on their surface. Cytotoxicity of CB has been well studied but the membrane damage mechanisms and role of surface modification are still open to debate. Negatively and positively charged giant unilamellar vesicles (GUVs) were prepared using three lipids as model cell membranes to examine the mechanistic damage of CB and MCB (modified by acidic potassium permanganate) aggregates. Optical images showed that both anionic CB and MCB disrupted the positively charged but not the negatively charged GUVs. This disruption deteriorated with the rise and extension of exposure concentration and time. Lipids extraction caused by CBNs (CB and MCB together are called CBNs) was found. MCB caused more severe disruption than CB. MCB was enveloped into vesicles through an endocytosis-like process at 120 mg/L. MCB mediated the gelation of GUVs, perhaps through C-O-P bonding bridges. The lower hydrodynamic diameter and more negative charges may have been responsible for the distinction effect of MCB over CB. The adhesion and bonding of CBNs to the membrane were favored by electrostatic interaction and the practical application of CBNs warrants more attention.
Adverse Childhood Experiences, Resilience, and Emotional Problems in Young Chinese Children
Authors: Zhu Yantong , Zhang Gengli , Anme Tokie
Published: February 2023

Resilience plays an important role in the relationship between adverse childhood experiences (ACEs) and children’s health. Young children are often neglected in ACEs research and suffer from the negative consequences of ACEs. However, few studies have focused on the relationship between ACEs and emotional problems in young Chinese children and the moderating and mediating effect of resilience on this relationship. This study included young children at the beginning of their kindergarten year (n = 874, 42.80 ± 4.09 months) from Wuhu City, China, to examine the mediation and moderation effects of resilience on early-life ACEs and emotional problems. Our results show a positive direct effect of ACEs on emotional problems. Furthermore, a positive indirect effect of ACEs and emotional problems on resilience was found. A moderating effect of resilience was not observed in this study. Our findings (a) highlight the significance of paying more attention to early ACEs and revealing a better understanding of the effect of resilience on ACEs at an early age and (b) indicate that age-specific interventions should be provided to enhance young children’s resilience when exposed to adversity.
Inhibition of Sphingosine-1-Phosphate Receptor 2 by JTE013 Enhanced Alveolar Bone Regeneration by Promoting Angiogenesis
Authors: Lory William , Wellslager Bridgette , Sun Chao , Yilmaz Özlem , Yu Hong
Published: February 2023

Sphingosine-1-phosphate receptor 2 (S1PR2) is a G protein-coupled receptor that regulates various immune responses. Herein, we report the effects of a S1PR2 antagonist (JTE013) on bone regeneration. Murine bone marrow stromal cells (BMSCs) were treated with dimethylsulfoxide (DMSO) or JTE013 with or without infection by an oral bacterial pathogen Aggregatibacter actinomycetemcomitans. Treatment with JTE013 enhanced vascular endothelial growth factor A (VEGFA), platelet derived growth factor subunit A (PDGFA), and growth differentiation factor 15 (GDF15) gene expression and increased transforming growth factor beta (TGFβ)/Smad and Akt signaling. Eight-week-old male C57BL/6J mice were challenged with ligatures around the left maxillary 2nd molar for 15 days to induce inflammatory bone loss. After ligature removal, mice were treated with diluted DMSO or JTE013 in the periodontal tissues 3 times per week for 3 weeks. Calcein was also injected twice to measure bone regeneration. Micro-CT scanning of maxillary bone tissues and calcein imaging revealed that treatment with JTE013 enhanced alveolar bone regeneration. JTE013 also increased VEGFA, PDGFA, osteocalcin, and osterix gene expressions in the periodontal tissues compared to control. Histological examination of periodontal tissues revealed that JTE013 promoted angiogenesis in the periodontal tissues compared to control. Our findings support that inhibition of S1PR2 by JTE013 increased TGFβ/Smad and Akt signaling; enhanced VEGFA, PDGFA, and GDF15 gene expression; and subsequently promoted angiogenesis and alveolar bone regeneration.
Mechanical, Barrier and Thermal Properties of Amylose-Argan Proteins-Based Bioplastics in the Presence of Transglutaminase
Authors: Famiglietti Michela , Zannini Domenico , Turco Rosa , Mariniello Loredana
Published: February 2023

The bioeconomy aims to discover new sources for producing energy and materials and to valorize byproducts that otherwise would get wasted. In this work, we investigate the possibility of producing novel bioplastics, made up of argan seed proteins (APs), extracted from argan oilcake, and amylose (AM), obtained from barley plants through an RNA interference technique. Argan, Argania spinosa, is a plant widespread in arid regions of Northern Africa, where it plays a fundamental socio-ecological role. Argan seeds are used to obtain a biologically active and edible oil, producing a byproduct, the oilcake, that is rich in proteins, fibers, and fats, and is generally used as animal food. Recently, argan oilcakes have been attracting attention as a waste to be recovered to obtain high-added-value products. Here, APs were chosen to test the performance of blended bioplastics with AM, because they have the potential to improve the properties of the final product. High-AM-starches present attractive features for use as bioplastics, including a higher gel-forming capacity, a higher thermal stability, and reduced swelling compared to normal starch. It has already been demonstrated that pure AM-based films provide more suitable properties than normal starch-based films. Here, we report on the performance of these novel blended bioplastics in terms of their mechanical, barrier, and thermal properties; and the effect of the enzyme microbial transglutaminase (mTGase) as a reticulating agent for AP’s components was also studied. These results contribute to the development of novel sustainable bioplastics with improved properties and confirm the possibility of valorizing the byproduct, APs, using them as a new raw material.
Artificial Intelligence-Assisted Meta-Analysis of the Frequency of ACE I/D Polymorphisms in Centenarians and Other Long-Lived Individuals
Authors: Li Lingxuan , Murakami Shin
Published: February 2023

Current research on the angiotensin-converting-enzyme (ACE) gene has yielded controversial results on whether different ACE polymorphisms are linked with human longevity. ACE polymorphisms are a risk factor for Alzheimer’s disease and age-onset diseases that may contribute to the mortality of older people. Our goal is to consolidate existing studies, using artificial intelligence-assisted software to come to a more precise understanding of the role of the ACE gene in human longevity. The I (insertion) and D (deletion) polymorphisms in the intron are correlated with the levels of circulating ACE; homozygous D (DD) is high, and homozygous I (II) is low. Here, we performed a detailed meta-analysis of the I and D polymorphisms using centenarians (100+ years old), long-lived subjects (85+ years old), and control groups. ACE genotype distribution was analyzed across a total of 2054 centenarians and 12,074 controls, as well as 1367 long-lived subjects between the ages of 85–99, using the inverse variance and random effects methods. The ACE DD genotype was found to be favored in centenarians (OR: 1.41 (95% CI: 1.19–1.67), p < 0.0001) with a heterogeneity of 32%, and the II genotype slightly favored the control groups (OR: 0.81 (95% CI: 0.66–0.98), p = 0.03) with a heterogeneity of 28%, corroborating results from previous meta-analyses. Novel to our meta-analysis, the ID genotype was found to be favored in control groups (OR: 0.86 (95% CI: 0.76–0.97), p = 0.01) with a heterogeneity of 0%. The long-lived group showed a similar positive association between the DD genotype and longevity (OR: 1.34 (95% CI: 1.21–1.48), p < 0.0001) and a negative association between the II genotype and longevity (OR: 0.79 (95% CI: 0.70–0.88), p < 0.0001). The long-lived ID genotype did not show significant findings (OR: 0.93 (95% CI: 0.84–1.02), p = 0.79). In conclusion, the results suggest a significant positive association of the DD genotype with human longevity. However, despite the previous study, the results do not confirm a positive association of the ID genotype with human longevity. We suggest a few important paradoxical implications: (1) inhibition of ACE can increase longevity in model systems from nematodes to mammals, seemingly opposite to the finding in humans; (2) exceptional longevity associated with homozygous DD is also associated with age-related diseases with higher mortality risks in homozygous DD. We discuss ACE, longevity, and age-related diseases.
Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review
Authors: Troha Kaja , Vozel Domen , Arko Matevž , Bedina Zavec Apolonija , Dolinar Drago , Hočevar Matej , Jan Zala , Kisovec Matic , Kocjančič Boštjan , Pađen Ljubiša , Pajnič Manca , Penič Samo , Romolo Anna , Repar Neža , Spasovski Vesna , Steiner Nejc , Šuštar Vid , Iglič Aleš , Drobne Damjana , Kogej Ksenija , Battelino Saba , Kralj-Iglič Veronika
Published: February 2023

The preparation of autologous platelet and extracellular vesicle-rich plasma (PVRP) has been explored in many medical fields with the aim to benefit from its healing potential. In parallel, efforts are being invested to understand the function and dynamics of PVRP that is complex in its composition and interactions. Some clinical evidence reveals beneficial effects of PVRP, while some report that there were no effects. To optimize the preparation methods, functions and mechanisms of PVRP, its constituents should be better understood. With the intention to promote further studies of autologous therapeutic PVRP, we performed a review on some topics regarding PVRP composition, harvesting, assessment and preservation, and also on clinical experience following PVRP application in humans and animals. Besides the acknowledged actions of platelets, leukocytes and different molecules, we focus on extracellular vesicles that were found abundant in PVRP.
Comparison of Glyphosate-Degradation Ability of Aldo-Keto Reductase (AKR4) Proteins in Maize, Soybean and Rice
Authors: Chen Ronghua , Wang Siwei , Sun Yue , Li Haiqing , Wan Shuqing , Lin Fei , Xu Hanhong
Published: February 2023

Genes that participate in the degradation or isolation of glyphosate in plants are promising, for they endow crops with herbicide tolerance with a low glyphosate residue. Recently, the aldo-keto reductase (AKR4) gene in Echinochloa colona (EcAKR4) was identified as a naturally evolved glyphosate-metabolism enzyme. Here, we compared the glyphosate-degradation ability of theAKR4 proteins from maize, soybean and rice, which belong to a clade containing EcAKR4 in the phylogenetic tree, by incubation of glyphosate with AKR proteins both in vivo and in vitro. The results indicated that, except for OsALR1, the other proteins were characterized as glyphosate-metabolism enzymes, with ZmAKR4 ranked the highest activity, and OsAKR4-1 and OsAKR4-2 exhibiting the highest activity among the AKR4 family in rice. Moreover, OsAKR4-1 was confirmed to endow glyphosate-tolerance at the plant level. Our study provides information on the mechanism underlying the glyphosate-degradation ability of AKR proteins in crops, which enables the development of glyphosate-resistant crops with a low glyphosate residue, mediated by AKRs.
Structure, Substrate Specificity and Role of Lon Protease in Bacterial Pathogenesis and Survival
Authors: Kirthika Perumalraja , Lloren Khristine Kaith Sison , Jawalagatti Vijayakumar , Lee John Hwa
Published: February 2023

Proteases are the group of enzymes that carry out proteolysis in all forms of life and play an essential role in cell survival. By acting on specific functional proteins, proteases affect the transcriptional and post-translational pathways in a cell. Lon, FtsH, HslVU and the Clp family are among the ATP-dependent proteases responsible for intracellular proteolysis in bacteria. In bacteria, Lon protease acts as a global regulator, governs an array of important functions such as DNA replication and repair, virulence factors, stress response and biofilm formation, among others. Moreover, Lon is involved in the regulation of bacterial metabolism and toxin–antitoxin systems. Hence, understanding the contribution and mechanisms of Lon as a global regulator in bacterial pathogenesis is crucial. In this review, we discuss the structure and substrate specificity of the bacterial Lon protease, as well as its ability to regulate bacterial pathogenesis.
Zebrafish as a Potential Model for Neurodegenerative Diseases: A Focus on Toxic Metals Implications
Authors: Paduraru Emanuela , Iacob Diana , Rarinca Viorica , Plavan Gabriel , Ureche Dorel , Jijie Roxana , Nicoara Mircea
Published: February 2023

In the last century, industrial activities increased and caused multiple health problems for humans and animals. At this moment, heavy metals are considered the most harmful substances for their effects on organisms and humans. The impact of these toxic metals, which have no biological role, poses a considerable threat and is associated with several health problems. Heavy metals can interfere with metabolic processes and can sometimes act as pseudo-elements. The zebrafish is an animal model progressively used to expose the toxic effects of diverse compounds and to find treatments for different devastating diseases that human beings are currently facing. This review aims to analyse and discuss the value of zebrafish as animal models used in neurological conditions, such as Alzheimer’s disease (AD), and Parkinson’s disease (PD), particularly in terms of the benefits of animal models and the limitations that exist.
Synergistic Antifungal Interactions between Antibiotic Amphotericin B and Selected 1,3,4-thiadiazole Derivatives, Determined by Microbiological, Cytochemical, and Molecular Spectroscopic Studies
Authors: Dróżdż Agnieszka , Kubera Dominika , Sławińska-Brych Adrianna , Matwijczuk Arkadiusz , Ślusarczyk Lidia , Czernel Grzegorz , Karcz Dariusz , Olender Alina , Bogut Agnieszka , Pietrzak Daniel , Dąbrowski Wojciech , Stepulak Andrzej , Wójcik-Załuska Alicja , Gagoś Mariusz
Published: February 2023

In recent years, drug-resistant and multidrug-resistant fungal strains have been more frequently isolated in clinical practice. This phenomenon is responsible for difficulties in the treatment of infections. Therefore, the development of new antifungal drugs is an extremely important challenge. Combinations of selected 1,3,4-thiadiazole derivatives with amphotericin B showing strong synergic antifungal interactions are promising candidates for such formulas. In the study, microbiological, cytochemical, and molecular spectroscopy methods were used to investigate the antifungal synergy mechanisms associated with the aforementioned combinations. The present results indicate that two derivatives, i.e., C1 and NTBD, demonstrate strong synergistic interactions with AmB against some Candida species. The ATR-FTIR analysis showed that yeasts treated with the C1 + AmB and NTBD + AmB compositions, compared with those treated with single compounds, exhibited more pronounced abnormalities in the biomolecular content, suggesting that the main mechanism of the synergistic antifungal activity of the compounds is related to a disturbance in cell wall integrity. The analysis of the electron absorption and fluorescence spectra revealed that the biophysical mechanism underlying the observed synergy is associated with disaggregation of AmB molecules induced by the 1,3,4-thiadiazole derivatives. Such observations suggest the possibility of the successful application of thiadiazole derivatives combined with AmB in the therapy of fungal infections.
The Potential of Senescence as a Target for Developing Anticancer Therapy
Authors: Jo Hyein , Shim Kyeonghee , Jeoung Dooil
Published: February 2023

Senescence occurs in response to various stimuli. Senescence has attracted attention because of its potential use in anticancer therapy as it plays a tumor-suppressive role. It also promotes tumorigeneses and therapeutic resistance. Since senescence can induce therapeutic resistance, targeting senescence may help to overcome therapeutic resistance. This review provides the mechanisms of senescence induction and the roles of the senescence-associated secretory phenotype (SASP) in various life processes, including therapeutic resistance and tumorigenesis. The SASP exerts pro-tumorigenic or antitumorigenic effects in a context-dependent manner. This review also discusses the roles of autophagy, histone deacetylases (HDACs), and microRNAs in senescence. Many reports have suggested that targeting HDACs or miRNAs could induce senescence, which, in turn, could enhance the effects of current anticancer drugs. This review presents the view that senescence induction is a powerful method of inhibiting cancer cell proliferation.