bluuebunny/crossref_metadata_2025 huggingface dataset:
Biogas improvement as renewable energy through conversion into methanol: A perspective of new catalysts based on nanomaterials and metal organic frameworks
Authors: Sánchez Antoni
Published: October 2022

In recent years, the high cost and availability of energy sources have boosted the implementation of strategies to obtain different types of renewable energy. Among them, methane contained in biogas from anaerobic digestion has gained special relevance, since it also permits the management of a big amount of organic waste and the capture and long-term storage of carbon. However, methane from biogas presents some problems as energy source: 1) it is a gas, so its storage is costly and complex, 2) it is not pure, being carbon dioxide the main by-product of anaerobic digestion (30%–50%), 3) it is explosive with oxygen under some conditions and 4) it has a high global warming potential (27–30 times that of carbon dioxide). Consequently, the conversion of biogas to methanol is as an attractive way to overcome these problems. This process implies the conversion of both methane and carbon dioxide into methanol in one oxidation and one reduction reaction, respectively. In this dual system, the use of effective and selective catalysts for both reactions is a critical issue. In this regard, nanomaterials embedded in metal organic frameworks have been recently tested for both reactions, with very satisfactory results when compared to traditional materials. In this review paper, the recent configurations of catalysts including nanoparticles as active catalysts and metal organic frameworks as support materials are reviewed and discussed. The main challenges for the future development of this technology are also highlighted, that is, its cost in environmental and economic terms for its development at commercial scale.
Disturbance of the Warburg effect by dichloroacetate and niclosamide suppresses the growth of different sub-types of malignant pleural mesothelioma in vitro and in vivo
Authors: Lam Sze-Kwan , Yan Sheng , Lam Joyce Sze-Man , Feng Yuqian , Khan Mahjabin , Chen Caoyang , Ko Frankie Chi-Fat , Ho James Chung-Man
Published: October 2022

Background: Inhalation of asbestos fibers is the most common cause of malignant pleural mesothelioma (MPM). In 2004, the United States Food and Drug Administration approved a combination of cisplatin with pemetrexed to treat unresectable MPM. Nonetheless novel treatment is urgently needed. The objective of this study is to report the combination effect of dichloroacetate (DCA) or niclosamide (Nic) Nic in MPM.

Materials and methods: The effect of a combination of DCA and Nic was studied using a panel of MPM cell lines (H28, MSTO-211H, H226, H2052, and H2452). Cell viability was monitored by MTT assay. Glycolysis, oxidative phosphorylation, glucose, glycogen, pyruvate, lactate, citrate, succinate and ATP levels were determined by corresponding ELISA. Apoptosis, mitochondrial transmembrane potential, cell cycle analysis, hydrogen peroxide and superoxide were investigated by flow cytometry. Cell migration and colony formation were investigated by transwell migration and colony formation assays respectively. The in vivo effect was confirmed using 211H and H226 nude mice xenograft models.

Results and conclusion: Cell viability was reduced. Disturbance of glycolysis and/or oxidative phosphorylation resulted in downregulation of glycogen, citrate and succinate. DCA and/or Nic increased apoptosis, mitochondrial transmembrane depolarization, G2/M arrest and reactive oxygen species. Moreover, DCA and/or Nic suppressed cell migration and colony formation. Furthermore, a better initial tumor suppressive effect was induced by the DCA/Nic combination compared with either drug alone in both 211H and H226 xenograft models. In H226 xenografts, DCA/Nic increased median survival of mice compared with single treatment. Single drug and/or a combination disturbed the Warburg effect and activated apoptosis, and inhibition of migration and proliferation in vivo. In conclusion, dichloroacetate and/or niclosamide showed a tumor suppressive effect in MPM in vitro and in vivo, partially mediated by disturbance of glycolysis/oxidative phosphorylation, apoptosis, ROS production, G2/M arrest, and suppression of migration and proliferation.
The protective roles of augmenter of liver regeneration in hepatocytes in the non-alcoholic fatty liver disease
Authors: Dong Yuan , Zhang Yuejie , Feng Yingmei , An Wei
Published: October 2022

Non-alcoholic fatty liver disease (NAFLD) occurs in 25% of the global population and manifests as lipid deposition, hepatocyte injury, activation of Kupffer and stellate cells, and steatohepatitis. Predominantly expressed in hepatocytes, the augmenter of liver regeneration (ALR) is a key factor in liver regulation that can alleviate fatty liver disease and protect the liver from abnormal liver lipid metabolism. ALR has three isoforms (15-, 21-, and 23-kDa), amongst which 23-kDa ALR is the most extensively studied. The 23-kDa ALR isoform is a sulfhydryl oxidase that resides primarily in the mitochondrial intermembrane space (IMS), whereby it protects the liver against various types of injury. In this review, we describe the role of ALR in regulating hepatocytes in the context of NAFLD. We also discuss questions about ALR that remain to be explored in the future. In conclusion, ALR appears to be a promising therapeutic target for treating NAFLD.
PpZAT5 suppresses the expression of a B-box gene PpBBX18 to inhibit anthocyanin biosynthesis in the fruit peel of red pear
Authors: Zhang Lu , Tao Ruiyan , Wang Simai , Gao Yuhao , Wang Lu , Yang Shulin , Zhang Xiao , Yu Wenjie , Wu Xinyue , Li Kunfeng , Ni Junbei , Teng Yuanwen , Bai Songling
Published: October 2022

BBX (B-box) proteins play a vital role in light-induced anthocyanin biosynthesis. PpBBX18 was an indispensable regulator for the induction of anthocyanin biosynthesis in the peel of red pear fruit (Pyrus pyrifolia Nakai.). However, the upstream regulation of BBX genes has not been well characterized. In this study, PpZAT5, a cysteine2/histidine2-type transcription factor, was discovered as the upstream negative regulator of PpBBX18. The results showed that PpZAT5 functions as a transcriptional repressor and directly binds to the CAAT motif of PpBBX18 and inhibits its expression. PpZAT5 expression was inhibited by light, which is converse to the expression pattern of anthocyanin-related structural genes. In addition, less anthocyanin accumulated in the PpZAT5-overexpressing pear calli than in the wild-type pear calli; on the contrary, more anthocyanin accumulated in PpZAT5-RNAi pear calli. Moreover, the crucial genes involved in light-induced anthocyanin biosynthesis were markedly down-regulated in the transcriptome of PpZAT5 overexpression pear calli compared to wild-type. In conclusion, our study indicates that PpBBX18 is negatively regulated by a C2H2-type transcriptional repressor, PpZAT5, which reduces anthocyanin content in pear. The present results demonstrate an upstream molecular mechanism of PpBBX18 and provide insights into light-induced anthocyanin biosynthesis.
Flow characteristics of open channels based on patch distribution of partially discontinuous rigid combined vegetation
Authors: Zhang Jingzhou , Zhang Shengtang , Wang Chuantao , Wang Wenjun , Ma Lijun
Published: October 2022

To clarify the flow characteristics of open channels under the combined distribution of vegetation in a patch, this study used the computational fluid dynamics tool FLUENT and the Reynolds stress model to design four combined and four discrete distribution modes under two different inundation states (submerged and non-submerged). The flow characteristics of longitudinally discontinuous rigid vegetation patches occupying half the width of the channel were numerically simulated. The numerical model is verified by indoor open channel flume experiments, and the obtained model data is in good agreement with the measured data. The results showed that: 1) The diameter of vegetation is an important factor affecting the wake structure. Under the submerged condition. 2)The submerged state, distribution pattern and combination form of vegetation are important factors that affect the distribution of flow velocity and change the structure of water flow. That is, the influence of vegetation distribution pattern on flow velocity and turbulence intensity under submerged condition is significantly weaker than that under non-submerged condition, and the flow velocity in non-vegetation area is significantly higher than that in vegetation area. The increase in the combined vegetation comprehensive stem thickness and the discrete degree resulted in an increase in the difference in flow velocity and turbulence intensity. 3) As the water flowed downstream, the flow velocity along the vegetated area continuously decreased, while it increased continuously along the non-vegetated area, and the difference in flow velocity between the two areas became more apparent. 4) The inundation state and combination characteristics of vegetation were important factors affecting the Reynolds stress of the channel location in the patch area.
In situ detection of fruit spoilage based on volatile compounds using the mid-infrared fiber-optic evanescent wave spectroscopy
Authors: Zhou Yunhai , Gu Yifan , Guo Rui , Jiao Leizi , Wang Ke , Zhu Qingzhen , Dong Daming
Published: October 2022

Volatile compounds such as ethanol released from fruit can be rapidly detected using Fourier Transform Infrared spectroscopy based on a long-path gas cell. However, this method relies on a long optical path length and requires pumping fruit volatiles into the gas cell. This can lead to the volatile compounds being contaminated and not detectable in situ. Fiber optic evanescent wave spectroscopy (FOEW) is not influenced by the path length so can detect materials (solid, liquid and gas phase) rapidly in situ, using only a few millimeters of optical fiber. In the present study, a spiral silver halide FOEW sensor with a length of approximately 21 mm was used to replace a long-path gas cell to explore the feasibility of identifying volatile compounds released from grapes in situ. The absorption peaks of ethanol in the volatile compounds were clearly found in the FOEW spectra and their intensity gradually increased as the storage time of the grapes increased. PCA analysis of these spectra showed clear clustering at different storage times (1-3, 4-5 and 6-7 d), revealing that the concentration of the ethanol released from the grapes changed significantly with time. The qualitative model established by PLS-DA algorithm could accurately classify grape samples as “Fresh,” “Slight spoilage,” or “Severe spoilage”. The accuracy of the calibration and validation sets both were 100.00%. These changes can therefore be used for rapidly identifying fruit deterioration. Compared with the method used in a previous study by the authors, this method avoids using a pumping process and can thus identify volatile compounds and hence monitor deterioration in situ and on-line by placing a very short optical fiber near the fruit.
Unpacking the interaction between foreign language learners’ emotion, cognition, and activity in the flipped classroom in higher education: A perezhivanie perspective
Authors: Qin Lili , Yao Lan , Jin Yinxing
Published: October 2022

Few studies have investigated learners’ emotional experiences and the interactions between emotion, cognition, and activity in the flipped foreign language classroom (i.e., a mixed teaching mode that combines in-class teaching and off-class self-directed learning). This study, from the perspective of perezhivanie (a concept from sociocultural theory), addressed these research gaps by exercising a longitudinal narrative study on a total of 32 Chinese-as-the-first-language university students of English who attended a 15-week English course with this teaching design. Among them, eight focal students were randomly selected for further evidence of the characteristics of the interactions between emotion, cognition, and activity. The results showed that the participants experienced more of positive emotions than negative emotions in the flipped classroom (FC) context, which supports the efficacy of the pedagogy. But most importantly, complex interactions between emotion, cognition, and activity were revealed. Generally, (negative) positive emotion, cognition, and activity were interconnected; however, what is also evident is that learners’ emotions either promoted or inhibited their cognitive functions, and positive and negative emotions did not necessarily correspond to positive and negative activities, respectively. This is due to the presence of dynamic, developmental, and historical sociocultural mediators in learners perezhivanija, be it teacher, peers, technology, teaching materials, teaching activities in an FC, or the learners’ previous English learning anecdotes, etc.
How live stream content types impact viewers’ support behaviors? Mediational analysis on psychological and social gratifications
Authors: Mao Eric
Published: October 2022

While previous research into live streaming was predominantly focused on video games, its content creation and provision has tremendously evolved, extending well-beyond game streams. Contents of general interest, such as e-commerce shopping, talent shows, and cute pets, started to prevail in today’s landscape of live streaming. However, limited attention has been given to how distinct types of streaming contents influence viewers’ psychological and behavioral responses. To fill this void, we employed an online survey (n = 583) to empirically examine the associations between popular live stream content types on Douyin (i.e., the TikTok app for China) and their viewers’ psychological and social gratifications and typical support behaviors. The results revealed that gratifications varied drastically across different content types. Game streaming, in particular, generated significant indirect impacts on all the support behaviors under consideration. On the other hand, whereas tension release served as a consistent mediator, the cognitive needs had no significant mediation effects. In sum, our study makes theoretical contributions to the literature by analyzing the thriving live stream phenomenon from a uses and gratifications perspective. We help augment the understanding of new media users’ preferences and choices in an attention economy, wherein human attention is conceptualized as a scarce resource. In practice, a better knowledge of viewer needs can facilitate streamers to customize their content creation and provision so as to accentuate elements of interest and elicit desired support behaviors (i.e., monetization opportunities).
Work–family and family–work conflict and stress in times of COVID-19
Authors: Elahi Natasha Saman , Abid Ghulam , Contreras Francoise , Fernández Ignacio Aldeanueva
Published: October 2022

This study aims to investigate the spillover impact of work-family/family–work conflict and stress on five major industrial sectors (education, textile, hospitals, banks, and retail stores), during the first wave of Covid-19. The purpose of this cross-sectional study is twofold; firstly, to test a hypothesized model where work-family/family-work conflicts are related to stress and where stress could exert a mediating role in such relationships. Secondly, we seek to explore the presence of these conflicts and stress in each of the five major industrial sectors and evaluate if there are significant differences between them, identifying the sociodemographic characteristics associated. Two questionnaires were applied to 748 employees from the selected industries. According to our results, stress predicts both types of conflict and also exerts a mediator role. It was primarily found that the five sectors are significantly different regarding the work-family/family-work conflicts and stress. Findings and implications are discussed.
Epidemiology, pathology, prevention, and control strategies of inclusion body hepatitis and hepatitis-hydropericardium syndrome in poultry: A comprehensive review
Authors: El-Shall Nahed A. , El-Hamid Hatem S. Abd , Elkady Magdy F. , Ellakany Hany F. , Elbestawy Ahmed R. , Gado Ahmed R. , Geneedy Amr M. , Hasan Mohamed E. , Jaremko Mariusz , Selim Samy , El-Tarabily Khaled A. , El-Hack Mohamed E. Abd
Published: October 2022

Infection with fowl adenoviruses (FAdVs) can result in a number of syndromes in the production of chicken, including inclusion body hepatitis (IBH), hepatitis-hydropericardium syndrome (HHS), and others, causing enormous economic losses around the globe. FAdVs are divided into 12 serotypes and five species (A–E; 1–8a and 8b−11). Most avian species are prone to infection due to the widespread distribution of FAdV strains. The genus aviadenovirus, which is a member of the adenoviridae family, is responsible for both IBH and HHS. The most popular types of transmission are mechanical, vertical, and horizontal. Hepatitis with basophilic intranuclear inclusion bodies distinguishes IBH, but the buildup of translucent or straw-colored fluid in the pericardial sac distinguishes HHS. IBH and HHS require a confirmatory diagnosis because their clinical symptoms and postmortem abnormalities are not unique to those conditions. Under a microscope, the presence of particular lesions and inclusion bodies may provide clues. Traditional virus isolation in avian tissue culture is more delicate than in avian embryonated eggs. Additionally, aviadenovirus may now be quickly and precisely detected using molecular diagnostic tools. Preventive techniques should rely on efficient biosecurity controls and immunize breeders prior to production in order to protect progeny. This current review gives a general overview of the current local and global scenario of IBH, and HHS brought on by FAdVs and covers both their issues and preventative vaccination methods.
Numerical Investigation on the Effect of Blockage on the Icing of Airfoils
Authors: Lu Daixiao , Lu Zhiliang , Han Zhirong , Xu Xian , Huang Ying
Published: October 2022

The blockage is one of the important factors affecting the icing of airfoils in wind tunnel tests. In this paper, numerical simulations are conducted to study the effect of blockage on the icing of different airfoils. By reducing the height of testing wind tunnels, the blockage is increased, and the changes in the height and angle of the ice horn are numerically investigated. The simulation results indicate that as the blockage increases, the flow velocity above the stagnation point of the airfoil increases, leading to larger pressure coefficients distribution and stronger heat transfer capacity. As a result, the position of icing moves forward, and the angle of the upper ice horn becomes smaller. In addition, the increased flow velocity facilitates the collection of water droplets in the area, which improves the icing and increases the height of the upper ice horn. It is also found that the blockage increases the angle of attack of the airfoil, moving the stagnation point backward and decreasing the angle of the upper ice horn. When the blockage is above 15%, the joint influence of the opening angle and height of the upper ice horn significantly reduces the projection height of the upper ice horn in the direction of the incoming flow, leading to unacceptable criticality of the ice shape.
Study on the Ablation Mechanism of the First Pulse Insulation Layer in a Double-Pulse Solid Rocket Motor
Authors: Zhang Kaining , Wang Chunguang , Tian Weiping
Published: October 2022

In this work, numerical simulation and experimental research were carried out on ablation mechanism of the first pulse (I pulse) insulation layer in a double-pulse solid rocket motor (SRM). Firstly, based on the internal thermal environment of the typical double pulse SRM, the internal flow field in combustion chamber of the double-pulse SRM with soft type pulse separation device (PSD) under the second pulse (Ⅱ pulse) working condition was numerically simulated. The results showed that the main reason for the difference of ablation in I pulse insulation layer was the difference of gas phase velocity. Secondly, based on the simulation analysis results, the experimental system for ablation of insulation layer was developed, and the ablation performance experiments under two gas phase velocities were carried out. It was found that a brittle carbonized layer had been formed on the surface of the insulation layer after the completion of I pulse work. In addition, at the beginning of Ⅱ pulse work, the suddenly generated gas flow made a denudation effect on the carbonized layer, which consumed a part of the carbonized layer. After the carbonized layer was peeled off, the gas flow continued to ablate the matrix of the insulation layer. Finally, the simulation analysis of the ablation process of the insulation layer under two gas phase velocities was carried out. The results showed that the velocity of the fuel gas is the main factor affecting the ablation rate of the insulation layer, which was consistent with the experimental results. It is proven that the model can be used to estimate the ablation amount of insulation of solid rocket motor. The conclusion can provide a significant reference for the internal heat protection design of the double-pulse SRM.
ASRS-CMFS vs. RoBERTa: Comparing Two Pre-Trained Language Models to Predict Anomalies in Aviation Occurrence Reports with a Low Volume of In-Domain Data Available
Authors: Kierszbaum Samuel , Klein Thierry , Lapasset Laurent
Published: October 2022

We consider the problem of solving Natural Language Understanding (NLU) tasks characterized by domain-specific data. An effective approach consists of pre-training Transformer-based language models from scratch using domain-specific data before fine-tuning them on the task at hand. A low domain-specific data volume is problematic in this context, given that the performance of language models relies heavily on the abundance of data during pre-training. To study this problem, we create a benchmark replicating realistic field use of language models to classify aviation occurrences extracted from the Aviation Safety Reporting System (ASRS) corpus. We compare two language models on this new benchmark: ASRS-CMFS, a compact model inspired from RoBERTa, pre-trained from scratch using only little domain-specific data, and the regular RoBERTa model, with no domain-specific pre-training. The RoBERTa model benefits from its size advantage, while the ASRS-CMFS benefits from the pre-training from scratch strategy. We find no compelling statistical evidence that RoBERTa outperforms ASRS-CMFS, but we show that ASRS-CMFS is more compute-efficient than RoBERTa. We suggest that pre-training a compact model from scratch is a good strategy for solving domain-specific NLU tasks using Transformer-based language models in the context of domain-specific data scarcity.
The Characteristics of Multilocus Sequence Typing, Virulence Genes and Drug Resistance of Klebsiella pneumoniae Isolated from Cattle in Northern Jiangsu, China
Authors: Xu Tianle , Wu Xinyue , Cao Hainan , Pei Tianxu , Zhou Yu , Yang Yi , Yang Zhangping
Published: October 2022

Klebsiella pneumoniae (K. pneumoniae) induced bovine mastitis has been becoming one of the dominantly pathogenic bacteria in cases of bovine mastitis, and is threatening public health through dairy products. In order to explore the characteristics of multilocus sequence typing (MLST), virulence gene carrying, and the relationship between virulence genes and the antibiotic resistance of Klebsiella pneumoniae from dairy cattle in northern Jiangsu, 208 dairy milk samples were collected from four dairy farms in northern Jiangsu. A total of 68 isolates were obtained through bacterial isolation, purification, and 16S rDNA identification. Eleven virulence genes were detected by specific PCR. The susceptibility of the isolates to antimicrobials was analyzed using the Kirby–Bauer method. The Pearson correlation coefficient was used to analyze the correlation between the presence of virulence genes and the phenotype of drug resistance. ST 2661 was the most prevalent type of K. pneumoniae (13/68, 19.1%) among the 23 ST types identified from the 68 isolates. The virulence gene allS was not detected, but the positive detection rates of the virulence genes fimH, ureA, uge and wabG were 100.0%. Notably, the detection rates of genes rmpA and wcaG, related to the capsular polysaccharide, were 4.4% and 11.8%, respectively, which were lower than those of genes related to siderophores (kfuBC, ybtA and iucB at 50.0%, 23.5%, and 52.9%, respectively). The K. pneumoniae isolates were sensitive to ciprofloxacin, nitrofurantoin, and meropenem. However, the resistance rate to penicillin was the highest (58/68, 85.3%), along with resistance to amoxicillin (16/68, 23.5%). The results revealed the distribution of 23 ST types of K. pneumoniae from the milk from bovine-mastitis-infected dairy cows in northern Jiangsu, and the expression or absence of the virulence gene kfuBC was related to the sensitivity to antibiotics. The current study provides important information relating to the distribution and characteristics of K. pneumoniae isolated from dairy cows with clinical bovine mastitis, and is indicative of strategies for improving the treatment of K. pneumoniae-induced bovine mastitis.
Foliage of Tropical Trees and Shrubs and Their Secondary Metabolites Modify In Vitro Ruminal Fermentation, Methane and Gas Production without a Tight Correlation with the Microbiota
Authors: Ángeles-Mayorga Yesenia , Cen-Cen Elmi Roseida , Crosby-Galván María Magdalena , Ramírez-Bribiesca Jacinto Efrén , Candelaria-Martínez Bernardino , Sánchez-Villarreal Alfredo , Ramírez-Mella Mónica
Published: October 2022

Ruminants, mainly cattle, contribute to greenhouse gases (GHG) emissions as methane (CH4) is produced by ruminal fermentation. Hence, various anti-methanogenic feed strategies have been studied, including the use of plants with secondary metabolites. This study evaluated in vitro ruminal fermentation metrics, microbial composition by digital droplet PCR (ddPCR) and the CH4 production of the foliage of several tropical trees and shrubs: Leucaena leucocephala, Moringa oleifera, Albizia lebbeck, Enterolobium cyclocarpum, Piscidia piscipula, Brosimum alicastrum, Lysiloma latisiliquum, Guazuma ulmifolia, Cnidoscolus aconitifolius, Gliricidia sepium and Bursera simaruba, using Cynodon plectostachyus grass as control. The results showed a wide variation in the chemical composition of the foliage, as well as in the ruminal microbiota. The crude protein (CP) content ranged from 11 to 25%, whereas the content of condensed tannins (CT) and saponins (S) was from 0.02 to 7%, and 3.2 to 6.6%, respectively. The greatest dry matter degradability (DMD) after 72 h was 69% and the least 35%, the latter coinciding with the least gas production (GP). A negative correlation was found between the CT and CH4 production, also between protozoa and fungi with the SGMT group of archaea. We concluded that the foliage of some tropical trees and shrubs has a high nutritional value and the potential to decrease CH4 production due to its CT content.
Differential Spleen miRNA Expression Profile of Beagle Dogs Infected with Toxocara canis
Authors: Xu Yue , Li Hao-Yu , Cai Lang , Xie Shi-Chen , Zou Yang , Zhu Xing-Quan , Zheng Wen-Bin
Published: October 2022

Toxocara canis is an unnoticed zoonotic helminth that causes severe disease in animals and humans. The spleen has a wide range of immunological functions in protecting the host against infection by many pathogens, but the function of the spleen in T. canis infection is still to be clarified, especially for the role of spleen microRNAs (miRNAs). In this study, deep sequencing of spleen RNA samples of 18 Beagle puppies was conducted to uncover the miRNAs expression profiling at 24 h post-infection (hpi), 96 hpi, and 36 days post infection (dpi). A total of 20, 34, and 19 differentially expressed miRNAs (DEmiRNAs) were identified at 24 hpi, 96 hpi, and 36 dpi, respectively. These DEmiRNAs (e.g., cfa-miR-206, cfa-miR-331, and cfa-miR-339) could play critical roles in Beagle puppies against T. canis infection, such as influencing inflammatory and immune-related cells and cytokines, by regulating target genes that are tightly associated with host immune function and enriched in immune response and immune pathways based on GO annotation and KEGG enrichment analysis. The current study discovered marked alterations of spleen miRNAs after T. canis infection, with potential effects on the pathogenesis of toxocariasis.
Effects of Stocking Rate and Environmental Enrichment on the Ontogeny of Pecking Behavior of Laying Hen Pullets Confined in Aviary Compartments during the First 4 Weeks of Life
Authors: Schwarzer Angela , Erhard Michael , Schmidt Paul , Zismann Miriam , Louton Helen
Published: October 2022

The objective of this study was to describe the ontogeny of the severe feather pecking (SFP), gentle feather pecking (GFP), aggressive pecking (AP), and enrichment pecking (EP) of non-beak-trimmed Lohmann Brown (LB)-pullets during the first 4 weeks of life (observation on 1st, 8th, 15th, 22nd, and 29th days of life) while they were kept within the compartments of a commercial rearing aviary (without access to a litter area). All chicks were placed into compartments of the middle tier of the aviary on the 1st day of life. On the 10th day of life, half of the chicks of each compartment were moved into the compartments of the lower tier. The aviary floor was covered with chick paper before the placement of the chicks and fully or partially removed from the 15th day of life onwards. The pecking behavior patterns were observed in three experimental groups (EG): NE (not enriched): group size until/after 10th day of life: 230/115; 120.8/60.4 birds/m2, no enrichment; EL (enriched, low stocking rate): group size until/after 10th day of life: 203/101, 106.6/53.6 birds/m2; and EH (enriched, high stocking rate): group size until/after 10th day of life: 230/115;120.8/60.4 birds/m2, both pecking stones and blocks as enrichment) in two rearing periods. For each pecking behavior pattern, an independent regression model with the parameters EG, chick paper, observation day, and functional area was estimated. GFP, SFP, and EP increased with age during the observation. The AP rate was highest in all EGs on the first day of life and decreased during the observation period. A pairwise comparison of NE (high stocking rate without enrichment) with EH (high stocking rate with enrichment and with EL (low stocking rate with enrichment) showed a significant effect of the EG on pecking behavior, with more SFP, AP, and GFP in NE. There were no differences between EL and EH, indicating that the provision of pecking materials had more influence than the stocking rate. However, we presumed that the difference between the stocking rates were too small to observe an effect. AP, SFP, and GFP were significantly higher on wired slats, as compared to the perches and the vicinity of the enrichment materials. The enrichment materials were suitable and intensely used by the pullets. The provision of pecking blocks and pecking stones was recommended as a preventive measure from the first day of life onwards for pullets housed in commercial rearing aviaries. There was no effect of reduced stocking rate, most likely due to the low variation in stocking rates.
Scale Difference from the Impact of Disease Control on Pig Production Efficiency
Authors: Hu Yaguan , Yu Yanli
Published: October 2022

Epidemic disease prevention plays a critical role in ensuring the healthy development of livestock farming, and the subjective willingness of breeders can be affected by the cost of epidemic disease prevention. To correct the misconception that farmers regard the cost of disease control as an ineffective cost, and to promote the healthy development of the pig breeding industry, our study employed the data envelopment analysis super-efficiency model and panel threshold regression model to evaluate the combination of the cost of epidemic disease prevention and swine productivity using data collected from 1998–2018 across 30 provinces in China. The following results were obtained. (1) The cost of epidemic disease prevention generated a non-linear on swine productivity when the swine farming scale was limited; (2) When the number of animals at the beginning of the year was less than 6.0002, swine productivity was impacted negatively; (3) When the number of animals at the beginning of the year ranged between 6.0002 and 12.9994, the impact was insignificant; (4) A strong correlation was observed between the expenses of epidemic disease prevention and animal productivity when the number of animals at the beginning of the year exceeded 12.9994. These results indicate that publicity should be enhanced to elucidate the combination of epidemic disease prevention and swine productivity among breeders. In addition, the government should introduce relevant policies to encourage the development of large-scale pig farming, such as subsidies for the construction of large-scale farms and insurance.
Spatial Factors Outperform Local Environmental and Geo-Climatic Variables in Structuring Multiple Facets of Stream Macroinvertebrates’ β-Diversity
Authors: Wu Naicheng , Liu Guohao , Zhang Min , Wang Yixia , Peng Wenqi , Qu Xiaodong
Published: October 2022

One of the key targets of community ecology and biogeography concerns revealing the variability and underlying drivers of biodiversity. Most current studies understand biodiversity based on taxonomic information alone, but few studies have shown the relative contributions of multiple abiotic factors in shaping biodiversity based on taxonomic, functional, and phylogenetic information. We collected 179 samples of macroinvertebrates in the Hun-Tai River Basin. We validated the complementarity between the three facets and components of β-diversity using the Mantel test. Distance-based redundancy analysis and variance partitioning were applied to explore the comparative importance of local environmental, geo-climatic, and spatial factors on each facet and component of β-diversity. Our study found that taxonomic and phylogenetic total β-diversity was mainly forced by turnover, while functional total β-diversity was largely contributed by nestedness. There is a strong correlation between taxonomic and phylogenetic β-diversity. However, the correlations of functional with both taxonomic and phylogenetic β-diversity were relatively weak. The findings of variation partitioning suggested that distinct facets and components of macroinvertebrates’ β-diversity were impacted by abiotic factors to varying degrees. The contribution of spatial factors was greater than that of the local environment and geo-climatic factors for taxonomic, functional, and phylogenetic β-diversity. Thus, studying different facets and components of β-diversity allows a clearer comprehension of the influence of abiotic factors on diversity patterns. Therefore, future research should investigate patterns and mechanisms of β-diversity from taxonomic, functional, and phylogenetic perspectives.
Ultrasonography of the Metacarpal/Tarsal-Phalangeal Joints in Healthy Racehorses: Normal Appearance, Breed-Related and Age-Related Features
Authors: Nocera Irene , Puccinelli Caterina , Sgorbini Micaela , Bagnoli Emma , Citi Simonetta
Published: October 2022

In adult horses, specific ultrasound (US) features and reference values have been reported for the appearance of the joint cartilage and thickness according to the type of joint, such as femoropatellar and tarsocrural. The US appearance of the fetlock has been described in several diseases. The present research evaluates the US features of the metacarpal/tarsal-phalangeal joints in healthy racehorses according to age and breed, since no information is available in the literature. Seventy-one fetlocks in 28 healthy horses (15/28 thoroughbreds and 13/28 standardbreds) were assessed. The horses were grouped as follows: group A < 5 years old vs. group B ≥ 5. A portable ultrasound machine and a linear transducer (5–7.5 MHz) were used. Dorsal metacarpal/tarsal-phalangeal joints were scanned. The US images were reviewed offline in terms of articular cartilage appearance, thickness, and subchondral bone appearance by an experienced observer. Data were reported as the median, minimum, and maximum for cartilage thickness values, and differences between groups were evaluated. Cartilage thickness values were statistically lower in group A than B in the standardbreds, except for the lateral thickness in longitudinal view. No differences were detected in the thoroughbreds within age groups. All of the young standardbreds showed a normal cartilage and subchondral appearance. No statistical differences were found between breeds. Our results highlight the characteristics of the US appearance of metacarpal/tarsal-phalangeal joints specifically in racehorses, with some variations according to age. Since the cartilage can change according to joint growth, age and training activity, the present findings suggest the use of specific references for US features, which are key to correctly evaluating the health of the fetlock.
Prediction of Indirect Indicators of a Grass-Based Diet by Milk Fourier Transform Mid-Infrared Spectroscopy to Assess the Feeding Typologies of Dairy Farms
Authors: Soyeurt Hélène , Gerards Cyprien , Nickmilder Charles , Bindelle Jérôme , Franceschini Sébastien , Dehareng Frédéric , Veselko Didier , Bertozzi Carlo , Gengler Nicolas , Marvuglia Antonino , Bayram Alper , Tedde Anthony
Published: October 2022

This research aims to develop a predictive model to discriminate milk produced from a cattle diet either based on grass or not using milk mid-infrared spectrometry and the month of testing (an indirect indicator of the feeding ration). The dataset contained 3,377,715 spectra collected between 2011 and 2021 from 2449 farms and 3 grazing traits defined following the month of testing. Records from 30% of the randomly selected farms were kept in the calibration set, and the remaining records were used to validate the models. Around 90% of the records were correctly discriminated. This accuracy is very good, as some records could be erroneously assigned. The probability of belonging to the GRASS modality allowed confirmation of the model’s ability to detect the transition period even if the model was not trained on this data. Indeed, the probability increased from the spring to the summer and then decreased. The discrimination was mainly explained by the changes in the milk fat, mineral, and protein compositions. A hierarchical clustering from the averaged probability per farm and year highlighted 12 groups illustrating different management practices. The probability of belonging to the GRASS class could be used in a tool counting the number of grazing days.
Effect of Acute Cold Stress on Neuroethology in Mice and Establishment of Its Model
Authors: Hu Yajie , Liu Yang , Li Shize
Published: October 2022

Cold environment is an inevitable stress source for humans and livestock in cold areas, which easily induce a cold stress response and then cause a series of abnormal changes in energy metabolism, neuroendocrine system, behavior and emotion. Homeostasis is maintained by the unified regulation of the autonomic nervous system, endocrine system, metabolism and behavior under cold exposure. Behavior is an indispensable part of the functional regulation of the body to respond to environmental changes. At present, the behavioral changes caused by cold exposure are unclear or even chaotic due to the difficulty of defining cold stress. Therefore, this study aims to systematically observe the changes in spontaneous movement, exploratory behavior and anxiety of mice under different intensity cold exposure and summarize the characteristics and behavior traits combined with relevant blood physiological indexes under corresponding conditions. Mice models of cold stress with different intensities were established (cold exposure gradients were 22 °C, 16 °C, 10 °C and 4 °C, and time gradients of each temperature were 2 h, 4 h, 6 h, 8 h, 10 h and 12 h). After the corresponding cold exposure treatment, mice immediately carried out the open field test(OFT) and elevated plus maze test (PMT) to evaluate their spontaneous movement, exploratory behavior and anxiety. Subsequently, blood samples were collected and used for the determination of corticosterone (Cort), corticotropin-releasing hormone (CRH), epinephrine (E), norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) by enzyme-linked immunosorbent assay (ELISA). Spontaneous movement of mice increased under 22 °C cold exposure, but their exploration behavior did not significantly change, and their anxiety improved at the initial stage. The spontaneous movement and anxiety of mice increased in the initial stage and decreased in the later stage under cold exposure at 16, 10 and 4 °C and the exploratory behavior was inhibited. The hypothalamic–pituitary–adrenal (HPA) axis and locus coeruleus-noradrenergic (LC/NE) system were activated by cold stress and fluctuated with different intensities of cold exposure. Meanwhile, serum DA increased, and 5-HT was the opposite under different intensities of cold exposure. In conclusion, mild acute cold exposure promoted the spontaneous movement, increased exploratory behavior and improved anxiety. As the intensity of cold exposure increases, cold exposure had a negative effect on spontaneous movement, exploratory behavior and emotion. The physiological basis of these behavioral and emotional changes in mice under different intensity cold stimulation is the fluctuation of Cort, CRH, E, NE, DA and 5-HT.
Tetra-Primer Amplification-Refractory Mutation System (ARMS)—PCR for Genotyping Mouse Leptin Gene Mutation
Authors: Chen Jiangang , Xu Xinyun , Dalhaimer Paul , Zhao Ling
Published: October 2022

Due to spontaneous deficiency in leptin, ob/ob mice are one of the most commonly used experimental animal models in diabetes research. In this study, we reported a quick and easy-to-conduct genotyping method using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) to differentiate mice with a mutated allele from the wild-type genotype. The amplicon patterns of different genotypes are clearly visible and distinguishable on 1.5% agarose gel. This method can serve as a valuable tool to differentiate genotypes for breeding purposes, to maintain animal colonies, control the available space in the animal facility, and identify appropriate individuals for animal experiments.
Whole-Genome Resequencing Highlights the Unique Characteristics of Kecai Yaks
Authors: Kang Yandong , Guo Shaoke , Wang Xingdong , Cao Mengli , Pei Jie , Li Ruiwu , Bao Pengjia , Wang Jiefeng , Lamao Jiebu , Gongbao Dangzhi , Lamao Ji , Liang Chunnian , Yan Ping , Guo Xian
Published: October 2022

Kecai yaks are regarded as an important genetic resource in China owing to their high fecundity and flavorful meat. However, the genetic characteristics of Kecai yaks have not been effectively characterized to date, and the relationship between Kecai yaks and other yak breeds remains to be fully characterized. In this paper, the resequencing of the Kecai yak genome is performed leading to the identification of 11,491,383 high-quality single nucleotide polymorphisms (SNPs). Through principal component, phylogenetic, and population genetic structure analyses based on these SNPs, Kecai yaks were confirmed to represent an independent population of yaks within China. In this study, marker and functional enrichment analysis of genes related to positive selection in Kecai yak was carried out, and the results show that such selection in Kecai yaks is associated with the adaptation to alpine environments and the deposition of muscle fat. Overall, these results offer a theoretical foundation for the future utilization of Kecai yak genetic resources.
Identification of a Small Molecule That Inhibits the Interaction of LPS Transporters LptA and LptC
Authors: Dai Xiaowei , Yuan Min , Lu Yu , Zhu Xiaohong , Liu Chao , Zheng Yifan , Si Shuyi , Yuan Lijie , Zhang Jing , Li Yan
Published: October 2022

The need for novel antibiotics has become imperative with the increasing prevalence of antibiotic resistance in Gram-negative bacteria in clinics. Acting as a permeability barrier, lipopolysaccharide (LPS) protects Gram-negative bacteria against drugs. LPS is synthesized in cells and transported to the outer membrane (OM) via seven lipopolysaccharide transport (Lpt) proteins (LptA–LptG). Of these seven Lpt proteins, LptC interacts with LptA to transfer LPS from the inner membrane (IM) to the OM, and assembly is aided by LptD/LptE. This interaction among the Lpt proteins is important for the biosynthesis of LPS; therefore, the Lpt proteins, which are significant in the assembly process of LPS, can be a potential target for new antibiotics. In this study, a yeast two-hybrid (Y2H) system was used to screen compounds that could block LPS transport by inhibiting LptA/LptC interaction, which finally disrupts the biosynthesis of the OM. We selected the compound IMB-0042 for this study. Our results suggest that IMB-0042 disrupts LptA/LptC interaction by binding to both LptA and LptC. Escherichia coli cells, when treated with IMB-0042, showed filament morphology, impaired OM integrity, and an accumulation of LPS in the periplasm. IMB-0042 inhibited the growth of Gram-negative bacteria and showed synergistic sensitization to other antibiotics, with low cytotoxicity. Thus, we successfully identified a potential antibacterial agent by using a Y2H system, which blocks the transport of LPS by targeting LptA/LptC interaction in Escherichia coli.
Characterization of Resistance and Virulence of Pasteurella multocida Isolated from Pet Cats in South China
Authors: Lin Haoyi , Liu Zhihui , Zhou Yingchun , Lu Weiguo , Xu Qian
Published: October 2022

Pasteurella multocida, one of the major zoonotic pathogens, may cause localized cellulitis, bacteremia, septic shock, and other symptoms in people. Accidental bites or scratches in close contact between humans and cats are one of the causes of P. multocida infection in humans. The prevalence of diseases due to P. multocida in humans may be rising as a result of the popularity of cat cafes in China. However, the epidemiology of P. multocida in cat-themed cafes in South China is yet to be reported. P. multocida in 92 healthy cats from four cafes with pet-cat themes was examined in their tonsils and oral cavities. The antibiotic susceptibility of each isolate was evaluated by using the disk-susceptibility testing method. The 19 P. multocida were selected for whole-genome sequencing (WGS), together with the genome data of eight previously described clinical strains isolated from humans, and the analysis of antimicrobial resistance (AMR) determinants, capsular genotyping, MLST genotyping, and virulence gene profiles was carried out. The results showed that 48.91% of cats (45/90) were positive for P. multocida. All isolated P. multocida stains were highly resistant to erythromycin (15 μg) (100%) and nonsensitive to fluoroquinolones (5 μg) (37.38%), while they were all susceptible to penicillin (10 untis), tetracycline (30 μg), doxycycline (30 μg), and chloramphenicol (30 μg). According to WGS analysis, those with the gyrA resistance gene were all insensitive to fluoroquinolone antibiotics. Virulence gene profiles showed that the genes pfhA, nanH, and plpB were practically all present in cats compared to humans. To conclude, increased antibiotic resistance, along with virulence factors, complicates the treatment of P. multocida infection. Thus, clinical treatment for P. multocida infection should be performed more cautiously.