bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Previous Alkylating Agent Exposure on Follicle Numbers in Cryopreserved Prepubertal and Young Adult Ovarian Tissue after Long-Term Xenografting
Authors: Nurmio Mirja , Asadi-Azarbaijani Babak , Hou Mi , Kiviö Ronja , Toppari Jorma , Tinkanen Helena , Laine Tiina , Oskam Irma C. , Jahnukainen Kirsi
Published: January 2022

Purpose and methods: To elucidate whether previous cancer treatment affects graft recovery and follicle numbers, morphology, and development in grafts, cryopreserved ovarian biopsies obtained from 18 cancer patients aged 1–24 years with and without exposure to chemotherapy were xenografted as 1 mm3 fragments to immunodeficient mice for 22 weeks with exogenous stimulation. Results: Graft recovery showed no association with chemotherapy exposure, pubertal stage, or leukemia contamination. Total follicle number per recovered graft varied between 0 and 1031 in the chemotherapy-exposed and between 0 and 502 in the non-chemotherapy-exposed group. Atretic follicles formed the largest proportion of the follicle pool in chemotherapy-exposed grafts. Increased atresia correlated with exposure to alkylating agents (mean ± SD 8866.2 ± 9316.3 mg/m2) but not with anthracyclines, pubertal stage, or leukemia contamination. Conclusion: The observation confirms the harmful effects of alkylating agents on ovarian tissue. Therapy at the median cumulative dose of 8866 mg/m2 leads to the decreased quality of cryopreserved ovarian follicles in children and young adults.
Intensity-Modulated Radiotherapy with Regional Hyperthermia for High-Risk Localized Prostate Carcinoma
Authors: Nakahara Sota , Ohguri Takayuki , Kakinouchi Sho , Itamura Hirohide , Morisaki Takahiro , Tani Subaru , Yahara Katuya , Fujimoto Naohiro
Published: January 2022

Background: The purpose of this study was to evaluate the efficacy and toxicity of adding regional hyperthermia to intensity-modulated radiotherapy (IMRT) plus neoadjuvant androgen deprivation therapy (ADT) for high-risk localized prostate carcinoma. Methods: Data from 121 consecutive patients with high-risk prostate carcinoma who were treated with IMRT were retrospectively analyzed. The total planned dose of IMRT was 76 Gy in 38 fractions for all patients; hyperthermia was used in 70 of 121 patients. Intra-rectal temperatures at the prostate level were measured to evaluate thermal dose. Results: Median number of heating sessions was five and the median total thermal dose of CEM43T90 was 7.5 min. Median follow-up duration was 64 months. Addition of hyperthermia to IMRT predicted better clinical relapse-free survival. Higher thermal dose with CEM43T90 (>7 min) predicted improved biochemical disease-free survival. The occurrence of acute and delayed toxicity ≥Grade 2 was not significantly different between patients with or without hyperthermia. Conclusions: IMRT plus regional hyperthermia represents a promising approach with acceptable toxicity for high-risk localized prostate carcinoma. Further studies are needed to verify the efficacy of this combined treatment.
Synthesis of Conjugated Dienes in Natural Compounds
Authors: Dumonteil Geoffrey , Berteina-Raboin Sabine
Published: January 2022

This review describes the various synthetic methods commonly used to obtain molecules possessing conjugated dienes. We focus on methods involving cross-coupling reactions using various metals such as nickel, palladium, ruthenium, cobalt, cobalt/zinc, manganese, zirconium, or iron, mainly through examples that aimed to access natural molecules or their analogues. Among the natural molecules covered in this review, we discuss the total synthesis of a phytohormone, Acid Abscisic (ABA), carried out by our team involving the development of a conjugated diene chain.
Heterocycles by Consecutive Multicomponent Syntheses via Catalytically Generated Alkynoyl Intermediates
Authors: Niedballa Jonas , Müller Thomas J. J.
Published: January 2022

Multicomponent processes are beneficial tools for the synthesis of heterocycles. As densely substituted bifunctional electrophiles, ynones are essential intermediates by applying cyclocondensations or cycloadditions in numerous heterocycle syntheses. The respective alkynoyl intermediates are generally accessible by palladium-, copper- and palladium/copper-catalyzed alkynylation. In turn, the mild reaction conditions allow for a fast and versatile entry to functional heterocycles in the sense of consecutive multicomponent processes. This review collates and presents recent advances in accessing thirteen heterocycle classes and their applications by virtue of catalytic alkynoyl generation in diversity-oriented multicomponent syntheses in a one-pot fashion.
Regulation of Leaf Angle Protects Photosystem I under Fluctuating Light in Tobacco Young Leaves
Authors: Zeng Zhi-Lan , Sun Hu , Wang Xiao-Qian , Zhang Shi-Bao , Huang Wei
Published: January 2022

Fluctuating light is a typical light condition in nature and can cause selective photodamage to photosystem I (PSI). The sensitivity of PSI to fluctuating light is influenced by the amplitude of low/high light intensity. Tobacco mature leaves are tended to be horizontal to maximize the light absorption and photosynthesis, but young leaves are usually vertical to diminish the light absorption. Therefore, we tested the hypothesis that such regulation of the leaf angle in young leaves might protect PSI against photoinhibition under fluctuating light. We found that, upon a sudden increase in illumination, PSI was over-reduced in extreme young leaves but was oxidized in mature leaves. After fluctuating light treatment, such PSI over-reduction aggravated PSI photoinhibition in young leaves. Furthermore, the leaf angle was tightly correlated to the extent of PSI photoinhibition induced by fluctuating light. Therefore, vertical young leaves are more susceptible to PSI photoinhibition than horizontal mature leaves when exposed to the same fluctuating light. In young leaves, the vertical leaf angle decreased the light absorption and thus lowered the amplitude of low/high light intensity. Therefore, the regulation of the leaf angle was found for the first time as an important strategy used by young leaves to protect PSI against photoinhibition under fluctuating light. To our knowledge, we show here new insight into the photoprotection for PSI under fluctuating light in nature.
Generation of a NES-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene Editing
Authors: Nouri Parivash , Zimmer Anja , Brüggemann Stefanie , Friedrich Robin , Kühn Ralf , Prakash Nilima
Published: January 2022

Advances in the regenerative stem cell field have propelled the generation of tissue-specific cells in the culture dish for subsequent transplantation, drug screening purposes, or the elucidation of disease mechanisms. One major obstacle is the heterogeneity of these cultures, in which the tissue-specific cells of interest usually represent only a fraction of all generated cells. Direct identification of the cells of interest and the ability to specifically isolate these cells in vitro is, thus, highly desirable for these applications. The type VI intermediate filament protein NESTIN is widely used as a marker for neural stem/progenitor cells (NSCs/NPCs) in the developing and adult central and peripheral nervous systems. Applying CRISPR-Cas9 technology, we have introduced a red fluorescent reporter (mScarlet) into the NESTIN (NES) locus of a human induced pluripotent stem cell (hiPSC) line. We describe the generation and characterization of NES-mScarlet reporter hiPSCs and demonstrate that this line is an accurate reporter of NSCs/NPCs during their directed differentiation into human midbrain dopaminergic (mDA) neurons. Furthermore, NES-mScarlet hiPSCs can be used for direct identification during live cell imaging and for flow cytometric analysis and sorting of red fluorescent NSCs/NPCs in this paradigm.
Advances in Allogeneic Cancer Cell Therapy and Future Perspectives on “Off-the-Shelf” T Cell Therapy Using iPSC Technology and Gene Editing
Authors: Furukawa Yoshiki , Hamano Yasuharu , Shirane Shuichi , Kinoshita Shintaro , Azusawa Yoko , Ando Jun , Nakauchi Hiromitsu , Ando Miki
Published: January 2022

The concept of allogeneic cell therapy was first presented over 60 years ago with hematopoietic stem cell transplantation. However, complications such as graft versus host disease (GVHD) and regimen-related toxicities remained as major obstacles. To maximize the effect of graft versus leukemia, while minimizing the effect of GVHD, donor lymphocyte infusion was utilized. This idea, which was used against viral infections, postulated that adoptive transfer of virus-specific cytotoxic T lymphocytes could reconstitute specific immunity and eliminate virus infected cells and led to the idea of banking third party cytotoxic T cells (CTLs). T cell exhaustion sometimes became a problem and difficulty arose in creating robust CTLs. However, the introduction of induced pluripotent stem cells (iPSCs) lessens such problems, and by using iPSC technology, unlimited numbers of allogeneic rejuvenated CTLs with robust and proliferative cytotoxic activity can be created. Despite this revolutionary concept, several concerns still exist, such as immunorejection by recipient cells and safety issues of gene editing. In this review, we describe approaches to a feasible “off-the-shelf” therapy that can be distributed rapidly worldwide. We also offer perspectives on the future of allogeneic cell cancer immunotherapy.
Photoelectron Circular Dichroism as a Probe of Chiral Hydrocarbons
Authors: Decleva Piero
Published: January 2022

The sensitivity of Photoelectron Circular Dichroism (PECD) in the angular distribution of photoelectrons, a recent chiral technique, to detect chirality in pure hydrocarbons is investigated in a number of benchmark molecules. It is found that a very large chiral signal is expected, surpassing most current examples, giving a sure fingerprint of absolute configuration. On the other hand, the sensitivity to specific isomers or closely related molecules is relatively modest.
Depressive Symptoms, Alcohol Beliefs and Heavy Episodic Drinking in Adolescents
Authors: Wellman Robert J. , Sabiston Catherine M. , Morgenstern Matthis
Published: January 2022

Adolescents who engage in heavy episodic drinking (HED—i.e., 5+ drinks on a single occasion) increase risks for psychopathology, alcohol dependence, and similar negative consequences in adulthood. We explored associations among depressive symptoms, positive alcohol beliefs, and progression of heavy episodic drinking (HED) in 3021 German adolescents (M(SD) age at baseline = 12.4 (1.0)) followed for 30 months in 4 waves, using a conditional parallel process linear growth model, with full information maximum likelihood estimation. By wave 4, 40.3% of participants had engaged in HED more than once; 16.4% had done so ≥5 times. Depressive symptoms were indirectly related to baseline values of HED (through positive beliefs and wave 1 drinking frequency and quantity) and to the rate of growth in HED (through positive beliefs and wave 1 quantity). Adolescents with higher levels of depressive symptoms and positive alcohol beliefs drink more frequently and at greater quantities, which is associated with initiating HED at a higher level and escalating HED more rapidly than peers with similar depressive symptoms who lack those beliefs. This suggests that, to the extent that positive alcohol beliefs can be tempered through public health campaigns, education and/or counseling, HED among depressed adolescents might be reduced.
Development of a Computational Tool for the Estimation of Alveolar Bone Loss in Oral Radiographic Images
Authors: Maithri M. , Ballal Dhanush G. , Kumar Santhosh , Raghavendra U. , Gudigar Anjan , Chan Wai Yee , Macherla Shravya , Vineetha Ravindranath , Gopalkrishna Pratibha , Ciaccio Edward J. , Acharya U. Rajendra
Published: January 2022

The present study evaluated a newly developed computational tool (CT) to assess the alveolar bone space and the alveolar crest angle and compares it to dentist assessment (GT). The novel tool consisted of a set of processes initiated with image enhancement, points localization, and angle and area calculations. In total, we analyzed 148 sites in 39 radiographic images, and among these, 42 sites were selected and divided into two groups of non-periodontitis and periodontitis. The alveolar space area (ASA) and alveolar crest angle (ACA) were estimated. The agreement between the computer software and the ground truth was analyzed using the Bland–Altman plot. The sensitivity and specificity of the computer tool were measured using the ROC curve. The Bland–Altman plot showed an agreement between the ground truth and the computational tool in all of the parameters assessed. The ROC curve showed 100% sensitivity and 100% specificity for 12.67 mm of the alveolar space area. The maximum percentage of sensitivity and specificity were 80.95% for 13.63 degrees of the alveolar crest angle. Computer tool assessment provides accurate disease severity and treatment monitoring for evaluating the alveolar space area (ASA) and the alveolar crest angle (ACA).
Improved Modular Division Implementation with the Akushsky Core Function
Authors: Babenko Mikhail , Tchernykh Andrei , Kuchukov Viktor
Published: January 2022

The residue number system (RNS) is widely used in different areas due to the efficiency of modular addition and multiplication operations. However, non-modular operations, such as sign and division operations, are computationally complex. A fractional representation based on the Chinese remainder theorem is widely used. In some cases, this method gives an incorrect result associated with round-off calculation errors. In this paper, we optimize the division operation in RNS using the Akushsky core function without critical cores. We show that the proposed method reduces the size of the operands by half and does not require additional restrictions on the divisor as in the division algorithm in RNS based on the approximate method.
Investigation of UV Dye-Sensitized Solar Cells Based on Water Electrolyte: A New Insight for Wavelength-Selective Greenhouse
Authors: Albulescu Daiana , Ursu Daniel , Rusnac Lucian-Mircea , Nitu Sabina , Miclau Marinela , Vajda Melinda
Published: January 2022

The optimization of the photoactive electrode based on TiO2 with a complex architecture for UV dyes along with water-based electrolyte has successfully allowed us (i) to obtain a photovoltaic efficiency of the dye-sensitized solar cell with 1.45 times higher than the best efficiency reported for synthetic dye and 3 times for curcumin dye so far; (ii) transparency on the entire Photosynthetic Active Radiation domain; (iii) preserving high efficiency for lighting 1 sun (summer) and shading, especially for 60 mW/cm2, which represents the maximum illumination in the rest of the seasons. Our water-based dye-sensitized solar cells loaded with synthetic and natural UV dyes have revealed that the implementation of a dye-sensitized solar cell in autonomous greenhouses is a viable and inexpensive concept.
Annual Cycle of the Synechococcus spp. and Picoeukaryotic Growth and Loss Rates in a Subtropical Coastal Ecosystem
Authors: Ho Pei-Chi , Gong Gwo-Ching , Mukhanov Vladimir , Zhu Zhi-Yu , Tsai An-Yi
Published: January 2022

Seasonal variations in the picophytoplankton community structure (Synechococcus spp. and picoeukaryotes) were studied by flow cytometry in the coastal ecosystem of the subtropical western Pacific from October 2019 to September 2020. Synechococcus spp. was dominant in abundance during the study period, with its density ranging from 0.05 to 5.6 × 104 cells mL−1; its maximum occurred in July 2020. Picoeukaryotes were less abundant, with their density ranging from 0.2 to 13.6 × 103 cells mL−1. Their highest abundance was recorded in January 2020. The growth rates of Synechococcus spp. and picoeukaryotes ranged from −0.39 to 1.42 d−1 and 0.38 to 2.46 d−1, respectively, throughout the study period. Overall, the growth rate of the picoeukaryotes was significantly higher than that of Synechococcus spp. It is interesting to note that the grazing mortality of Synechococcus spp. and picoeukaryotes during the warmer period (April to September) was relatively low. Based on this study, we suggest that mixotrophic nanoflagellates lowered their feeding activity that obtained nutrients from prey and instead used additional nutrients during the incubation experiments. Our study demonstrated that a shift in the picophytoplankton community composition and grazing activity of predacious nanoflagellates in cold and warm periods can impact on the seasonal dynamics of the microbial food web.
Underwater Video as a Tool to Quantify Fish Density in Complex Coastal Habitats
Authors: Baker Ronald , Bilbrey Dakota , Bland Aaron , D’Alonzo Frank , Ehrmann Hannah , Havard Sharon , Porter Zoe , Ramsden Sarah , Rodriguez Alexandra R.
Published: January 2022

Habitat loss is a serious issue threatening biodiversity across the planet, including coastal habitats that support important fish populations. Many coastal areas have been extensively modified by the construction of infrastructure such as ports, seawalls, docks, and armored shorelines. In addition, habitat restoration and enhancement projects often include constructed breakwaters or reefs. Such infrastructure may have incidental or intended habitat values for fish, yet their physical complexity makes quantitatively sampling these habitats with traditional gears challenging. We used a fleet of unbaited underwater video cameras to quantify fish communities across a variety of constructed and natural habitats in Perdido and Pensacola Bays in the central northern Gulf of Mexico. Between 2019 and 2021, we collected almost 350 replicate 10 min point census videos from rock jetty, seawall, commercial, public, and private docks, artificial reef, restored oyster reef, seagrass, and shallow sandy habitats. We extracted standard metrics of Frequency of Occurrence and MaxN, as well as more recently developed MeanCount for each taxon observed. Using a simple method to measure the visibility range at each sampling site, we calculated the area of the field of view to convert MeanCount to density estimates. Our data revealed abundant fish assemblages on constructed habitats, dominated by important fisheries species, including grey snapper Lutjanus griseus and sheepshead Archosargus probatocephalus. Our analyses suggest that density estimates may be obtained for larger fisheries species under suitable conditions. Although video is limited in more turbid estuarine areas, where conditions allow, it offers a tool to quantify fish communities in structurally complex habitats inaccessible to other quantitative gears.
Morphostructural Characterization of the Heterogeneous Rhodolith Bed at the Marine Protected Area “Capo Carbonara” (Italy) and Hydrodynamics
Authors: Bracchi Valentina A. , Caronni Sarah , Meroni Agostino N. , Burguett Esteban Gottfried , Atzori Fabrizio , Cadoni Nicoletta , Marchese Fabio , Basso Daniela
Published: January 2022

Mediterranean rhodolith beds are priority marine benthic habitats for the European Community, because of their relevance as biodiversity hotspots and their role in the carbonate budget. Presently, Mediterranean rhodolith beds typically occur within the range of 30–75 m of water depth, generally located around islands and capes, on flat or gently sloping areas. In the framework of a collaboration between the University of Milano-Bicocca and the Marine Protected Area “Capo Carbonara” (Sardinia, Italy), video explorations and sampling collections in three selected sites revealed the occurrence of a well developed and heterogeneous rhodolith bed. This bed covers an area >41 km2 around the cape, with live coverage ranging between 6.50 and 55.25%. Rhodoliths showed interesting morphostructural differences. They are small compact pralines at the Serpentara Island, associated with gravelly sand, or bigger boxwork at the Santa Caterina shoal associated with sand, whereas branches are reported mostly in the Is Piscadeddus shoal, associated with muddy sand. Both in the Santa Caterina shoal and the Serpentara Island, rhodoliths generally show a spheroidal shape, associated with a mean value of currents of 4.3 and 7.3 cm/s, respectively, up to a maximum of 17.7 cm/s at Serpentara, whereas in the Is Piscadeddus shoal rhodolith shape is variable and current velocity is significantly lower. The different hydrodynamic regime, with a constant current directed SW, which deviates around the cape towards E, is responsible for such morphostructural heterogeneity, with the site of the Serpentara Island being the most exposed to a constant unidirectional and strong current. We can associate current velocity with specific rhodolith morphotypes. The morphostructural definition of the heterogeneity of rhodoliths across large beds must be considered for appropriate management policies.
Single-Use and Reusable Flexible Bronchoscopes in Pulmonary and Critical Care Medicine
Authors: Ho Elliot , Wagh Ajay , Hogarth Kyle , Murgu Septimiu
Published: January 2022

Flexible bronchoscopy plays a critical role in both diagnostic and therapeutic management of a variety of pulmonary disorders in the bronchoscopy suite and the intensive care unit. In the set-ting of the ongoing viral pandemic, single-use flexible bronchoscopes (SUFB) have garnered attention as various professional pulmonary societies have released guidelines regarding uses for SUFB given the concern for risk of viral transmission when using reusable flexible bronchoscopes (RFB). In addition to offering sterility, SUFBs are portable, easily accessible, and may be more cost-effective than RFB when considering the potential costs of treating bronchoscopy-related infections. Furthermore, since SUFBs are one time use, they do not require reprocessing after use, and therefore may translate to reduced cleaning and storage costs. Despite these advantages, RFBs are still routinely used to perform advanced diagnostic and therapeutic bronchoscopic procedures given the need for optimal maneuverability, handling, angle of deflection, image quality, and larger channel size for passing of ancillary instruments. Here, we review the published evidence on the applications of single-use and reusable bronchoscopes in bronchoscopy suites and intensive care units. Specifically, we will discuss the advantages and disadvantages of these devices as pertinent to fundamental, advanced, and therapeutic bronchoscopic interventions.
Association of Trabecular Bone Score-Adjusted Fracture Risk Assessment Tool with Coronary Artery Calcification in Women
Authors: Chuang Tzyy-Ling , Wang Yuh-Feng , Koo Malcolm , Chuang Mei-Hua
Published: January 2022

The trabecular bone score (TBS) was found to be significantly associated with moderate coronary artery calcification (CAC). The aim of this study was to further explore the association between TBS-adjusted Fracture Risk Assessment Tool (FRAX) and CAC score in women. The electronic medical record database of a regional teaching hospital in southern Taiwan yielded women who received both coronary computed tomography and bone mineral density (BMD) measurement during their general health examination. Health history, anthropomorphic measurements, laboratory results, BMD, and T-scores were obtained. TBS values were calculated from database spine dual-energy X-ray absorptiometry files. Linear regression analyses tested the association between CAC score and 10-year probability of major osteoporotic fracture (MOF) and hip fracture (HF) determined by TBS-adjusted FRAX. Of the 116 women (mean age 55.8 years) studied, 24.1% had osteoporosis. Simple linear regression showed a significant association of CAC score with an increase in MOF and HF risk as measured by TBS-adjusted FRAX. In multiple linear regression adjusted for potential confounders, CAC score remained significantly associated with TBS-adjusted FRAX for right MOF (p = 0.002), left MOF (p = 0 006), right HF (p = 0.005), and left HF (p = 0.015). In conclusion, clinicians should be vigilant to the potential increased risk of coronary events among women with increased TBS-adjusted FRAX for MOF and HF.
High Sensitivity C Reactive Protein in Patients with Rheumatoid Arthritis Treated with Antibodies against IL-6 or Jak Inhibitors: A Clinical and Ultrasonographic Study
Authors: Frade-Sosa Beatriz , Ponce Andrés , Ruiz-Esquide Virginia , García-Yébenes Maria Jesús , Morlá Rosa , Sapena Nuria , Ramirez Julio , Azuaga Ana Belen , Sarmiento Juan Camilo , Cañete Juan D. , Gomez-Puerta Jose A. , Sanmarti Raimon
Published: January 2022

Background: We examined whether high-sensitivity CRP (hsCRP) reflected the inflammatory disease status evaluated by clinical and ultrasound (US) parameters in RA patients receiving IL-6 receptor antibodies (anti-IL-6R) or JAK inhibitors (JAKi). Methods: We conducted a cross-sectional study of patients with established RA receiving anti-IL-6R (tocilizumab, sarilumab) or JAKi (tofacitinib, baricitinib). Serum hsCRP and US synovitis in both hands were measured. Associations between hsCRP and clinical inflammatory activity were evaluated using composite activity indices. The association between hsCRP and US synovitis was analyzed. Results: 63 (92% female) patients (42 anti- IL-6R and 21 JAKi) were included, and the median disease duration was 14.4 (0.2–37.5) years. Most patients were in remission or had low levels of disease. Overall hsCRP values were very low, and significantly lower in anti-IL-6R patients (median 0.04 mg/dL vs. 0.16 mg/dL). Anti-IL-6R (82.4%) patients and 48% of JAKi patients had very low hsCRP levels (≤0.1 mg/dL) (p = 0.002). In the anti-IL-6R group, hsCRP did not correlate with the composite activity index or US synovitis. In the JAKi group, hsCRP moderately correlated with US parameters (r = 0.5) but not clinical disease activity, and hsCRP levels were higher in patients with US synovitis (0.02 vs. 0.42 mg/dL) (p = 0.001). Conclusion: In anti-IL-6R RA-treated patients, hsCRP does not reflect the inflammatory disease state, but in those treated with JAKi, hsCRP was associated with US synovitis.
Biomarkers in EndoVascular Aneurysm Repair (EVAR) and Abdominal Aortic Aneurysm: Pathophysiology and Clinical Implications
Authors: Stilo Francesco , Catanese Vincenzo , Nenna Antonio , Montelione Nunzio , Codispoti Francesco Alberto , Verghi Emanuele , Gabellini Teresa , Jawabra Mohamad , Chello Massimo , Spinelli Francesco
Published: January 2022

Circulating biomarkers have been recently investigated among patients undergoing endovascular aortic aneurysm repair (EVAR) for abdominal aortic aneurysm (AAA). Considering the plethora of small descriptive studies reporting potential associations between biomarkers and clinical outcomes, this review aims to summarize the current literature considering both the treated disease (post EVAR) and the untreated disease (AAA before EVAR). All studies describing outcomes of tissue biomarkers in patients undergoing EVAR and in patients with AAA were included, and references were checked for additional sources. In the EVAR scenario, circulating interleukin-6 (IL-6) is a marker of inflammatory reaction which might predict postoperative morbidity; cystatin C is a promising early marker of post-procedural acute kidney injury; plasma matrix metalloproteinase-9 (MMP-9) concentration after 3 months from EVAR might help in detecting post-procedural endoleak. This review also summarizes the current gaps in knowledge and future direction of this field of research. Among markers used in patients with AAA, galectin and granzyme appear to be promising and should be carefully investigated even in the EVAR setting. Larger prospective trials are required to establish and evaluate prognostic models with highest values with these markers.
A Machine Learning Ensemble Based on Radiomics to Predict BI-RADS Category and Reduce the Biopsy Rate of Ultrasound-Detected Suspicious Breast Masses
Authors: Interlenghi Matteo , Salvatore Christian , Magni Veronica , Caldara Gabriele , Schiavon Elia , Cozzi Andrea , Schiaffino Simone , Carbonaro Luca Alessandro , Castiglioni Isabella , Sardanelli Francesco
Published: January 2022

We developed a machine learning model based on radiomics to predict the BI-RADS category of ultrasound-detected suspicious breast lesions and support medical decision-making towards short-interval follow-up versus tissue sampling. From a retrospective 2015–2019 series of ultrasound-guided core needle biopsies performed by four board-certified breast radiologists using six ultrasound systems from three vendors, we collected 821 images of 834 suspicious breast masses from 819 patients, 404 malignant and 430 benign according to histopathology. A balanced image set of biopsy-proven benign (n = 299) and malignant (n = 299) lesions was used for training and cross-validation of ensembles of machine learning algorithms supervised during learning by histopathological diagnosis as a reference standard. Based on a majority vote (over 80% of the votes to have a valid prediction of benign lesion), an ensemble of support vector machines showed an ability to reduce the biopsy rate of benign lesions by 15% to 18%, always keeping a sensitivity over 94%, when externally tested on 236 images from two image sets: (1) 123 lesions (51 malignant and 72 benign) obtained from two ultrasound systems used for training and from a different one, resulting in a positive predictive value (PPV) of 45.9% (95% confidence interval 36.3–55.7%) versus a radiologists’ PPV of 41.5% (p < 0.005), combined with a 98.0% sensitivity (89.6–99.9%); (2) 113 lesions (54 malignant and 59 benign) obtained from two ultrasound systems from vendors different from those used for training, resulting into a 50.5% PPV (40.4–60.6%) versus a radiologists’ PPV of 47.8% (p < 0.005), combined with a 94.4% sensitivity (84.6–98.8%). Errors in BI-RADS 3 category (i.e., assigned by the model as BI-RADS 4) were 0.8% and 2.7% in the Testing set I and II, respectively. The board-certified breast radiologist accepted the BI-RADS classes assigned by the model in 114 masses (92.7%) and modified the BI-RADS classes of 9 breast masses (7.3%). In six of nine cases, the model performed better than the radiologist did, since it assigned a BI-RADS 3 classification to histopathology-confirmed benign masses that were classified as BI-RADS 4 by the radiologist.
Multiplex Lateral Flow Immunoassay for the Detection of Expanded-Spectrum Hydrolysis and CTX-M Enzymes
Authors: Moguet Christian , Gonzalez Camille , Naas Thierry , Simon Stéphanie , Volland Hervé
Published: January 2022

Background: Early detection of expanded-spectrum cephalosporinase (ESC) hydrolyzing ß-lactamases is essential for antibiotic stewardship. Here we have developed a multiplex lateral flow immunoassay (LFIA) that detects cefotaxime-hydrolyzing activity as well as the most prevalent ESC-hydrolyzing ß-lactamases: the CTX-M-like. Methods: The Rapid LFIA ESC test was evaluated retrospectively on 188 (139 Enterobacterales, 30 Pseudomonas spp. and 14 Acinetobacter spp.) agar-grown bacterial isolates with well-characterized ß-lactamase content. One single colony was resuspended in 150 µL extraction buffer containing cefotaxime, incubated at room temperature for 30 min prior to loading on the LFIA for reading within 10 min. Results: Out of the 188 isolates, all 17 that did not express a β-lactamase hydrolyzing cefotaxime gave negative results, and all 171 isolates expressing a β-lactamase known to hydrolyze cefotaxime, gave a positive test result. In addition, all 86 isolates expressing a CTX-M-variant belonging to one of the five CTX-M-subgroups were correctly identified. The sensitivity and specificity was 100% for both tests. Conclusions: The results showed that the multiplex LFIA was efficient, fast, low cost and easy to implement in routine laboratory work for the confirmation of ESC hydrolyzing activity and the presence of CTX-M enzymes.
Performance Enhancement of Optimized Link State Routing Protocol by Parameter Configuration for UANET
Authors: Tuli Esmot Ara , Golam Mohtasin , Kim Dong-Seong , Lee Jae-Min
Published: January 2022

The growing need for wireless communication has resulted in the widespread usage of unmanned aerial vehicles (UAVs) in a variety of applications. Designing a routing protocol for UAVs is paramount as well as challenging due to its dynamic attributes. The difficulty stems from features other than mobile ad hoc networks (MANET), such as aerial mobility in 3D space and frequently changing topology. This paper analyzes the performance of four topology-based routing protocols, dynamic source routing (DSR), ad hoc on-demand distance vector (AODV), geographic routing protocol (GRP), and optimized link state routing (OLSR), by using practical simulation software OPNET 14.5. Performance evaluation carries out various metrics such as throughput, delay, and data drop rate. Moreover, the performance of the OLSR routing protocol is enhanced and named “E-OLSR” by tuning parameters and reducing holding time. The optimized E-OLSR settings provide better performance than the conventional request for comments (RFC 3626) in the experiment, making it suitable for use in UAV ad hoc network (UANET) environments. Simulation results indicate the proposed E-OLSR outperforms the existing OLSR and achieves supremacy over other protocols mentioned in this paper.
A New Visual Inertial Simultaneous Localization and Mapping (SLAM) Algorithm Based on Point and Line Features
Authors: Zhang Tong , Liu Chunjiang , Li Jiaqi , Pang Minghui , Wang Mingang
Published: January 2022

In view of traditional point-line feature visual inertial simultaneous localization and mapping (SLAM) system, which has weak performance in accuracy so that it cannot be processed in real time under the condition of weak indoor texture and light and shade change, this paper proposes an inertial SLAM method based on point-line vision for indoor weak texture and illumination. Firstly, based on Bilateral Filtering, we apply the Speeded Up Robust Features (SURF) point feature extraction and Fast Nearest neighbor (FLANN) algorithms to improve the robustness of point feature extraction result. Secondly, we establish a minimum density threshold and length suppression parameter selection strategy of line feature, and take the geometric constraint line feature matching into consideration to improve the efficiency of processing line feature. And the parameters and biases of visual inertia are initialized based on maximum posterior estimation method. Finally, the simulation experiments are compared with the traditional tightly-coupled monocular visual–inertial odometry using point and line features (PL-VIO) algorithm. The simulation results demonstrate that the proposed an inertial SLAM method based on point-line vision for indoor weak texture and illumination can be effectively operated in real time, and its positioning accuracy is 22% higher on average and 40% higher in the scenario that illumination changes and blurred image.
Understanding the Conceptions of Engineering in Early Elementary Students
Authors: Lampley Sandra A. , Dyess Sarah Roller , Benfield Michael P. J. , Davis Andrew M. , Gholston Sampson E. , Dillihunt Monica L. , Turner Matthew W.
Published: January 2022

There is a demand for more STEM professionals. Early elementary students’ conceptions about engineering can influence whether or not they explore STEM career paths and ultimately select an engineering career. This study examined the conceptions elementary students have regarding the work that engineers perform. The research questions were the following: (1) what images do early elementary students associate with engineering and engineers, (2) do these associations vary from grade to grade, (3) are there gendered differences in these associations, and (4) how do the associations from this sample compare with the associations from the broader (grades one–five) Cunningham, Lachapelle, and Lindgren-Steider (2005) sample? Survey data from 1811 students in grades one–three were analyzed by comparison analysis and cluster analysis and then compared to the initial Cunningham et al. (2005) study. The results indicate two ways elementary students envision engineering: (a) creating designs or collecting and analyzing data, and (b) utilizing equipment to build and improve things. Comparison with the Cunningham et al. (2005) study suggests that there may be shifts in the way elementary students perceive engineering. Since these shifts could be attributed to a variety of factors, future work that determines what learning experiences might be contributing to students’ conceptions about engineering is recommended.
Drawing as a Space for Social-Cognitive Interaction
Authors: Andrade Vanessa De , Freire Sofia , Baptista Mónica , Shwartz Yael
Published: January 2022

Drawing is recognized as a powerful tool to learn science. Although current research has enriched our understanding of the potential of learning through drawing, scarce attention has been given to the social-cognitive interactions that occur when students jointly create drawings to understand and explain phenomena in science. This article is based on the distributed and embodied cognition theories and it adopted the notion of we-space, defined as a complex social-cognitive space, dynamically established and managed during the ongoing interactions of the individuals, when they manipulate and exploit a shared space. The goal of the study was to explore the role that collaborative drawing plays in shaping the social-cognitive interaction among students. We examine this by a fine-grain multimodal analysis of a pair of middle school students, who jointly attempted to understand and explain a chemical phenomenon by creating drawings and thinking with them. Our findings suggest that collaborative drawing played a key role in (i) establishing a genuine shared-action space, a we-space, and that within this we-space it had two major functions: (ii) enabling collective thinking-in-action and (iii) simplifying communication. We argue that drawing, as a joint activity, has a potential for learning, not restricted to the cognitive process related to the activity of creating external visual representations on paper; instead, the benefits of drawing lie in action in space. Creating these representations is more than a process of externalization of thought: it is part of a process of collective thinking-in-action.
Modeling Bitcoin plus Ethereum as an Open System of Systems of Public Blockchains to Improve Their Resilience against Intentional Risk
Authors: Partida Alberto , Gerassis Saki , Criado Regino , Romance Miguel , Giráldez Eduardo , Taboada Javier
Published: January 2022

In this article, we model the two most market-capitalised public, open and permissionless blockchain implementations, Bitcoin (BTC) and Ethereum (ETH), as a System of Systems (SoS) of public blockchains. We study the concepts of blockchain, BTC, ETH, complex networks, SoS Engineering and intentional risk. We analyse BTC and ETH from an open SoS perspective through the main properties that seminal System of Systems Engineering (SoSE) references propose. This article demonstrates that these public blockchain implementations create networks that grow in complexity and connect with each other. We propose a methodology based on a complexity management lever such as SoSE to better understand public blockchains such as BTC and ETH and manage their evolution. Our ultimate objective is to improve the resilience of public blockchains against intentional risk: a key requirement for their mass adoption. We conclude with specific measures, based on this novel systems engineering approach, to effectively improve the resilience against intentional risk of the open SoS of public blockchains, composed of a non-inflationary money system, “sound money”, such as BTC, and of a world financial computer system, “a financial conduit”, such as ETH. The goal of this paper is to formulate a SoS that transfers digital value and aspires to position itself as a distributed alternative to the fiat currency-based financial system.
Life Stage and Neighborhood-Dependent Survival of Longleaf Pine after Prescribed Fire
Authors: Magee Lukas , Pandit Karun , Flory Stephen Luke , Crandall Raelene M. , Broadbent Eben N. , Prata Gabriel A. , Dillon Whalen , Bohlman Stephanie , Johnson Daniel J.
Published: January 2022

Determining mechanisms of plant establishment in ecological communities can be particularly difficult in disturbance-dominated ecosystems. Longleaf pine (Pinus palustris Mill.) and its associated plant community exemplify systems that evolved with disturbances, where frequent, widespread fires alter the population dynamics of longleaf pine within distinct life stages. We identified the primary biotic and environmental conditions that influence the survival of longleaf pine in this disturbance-dominated ecosystem. We combined data from recruitment surveys, tree censuses, dense lidar point clouds, and a forest-wide prescribed fire to examine the response of longleaf pine individuals to fire and biotic neighborhoods. We found that fire temperatures increased with increasing longleaf pine neighborhood basal area and decreased with higher oak densities. There was considerable variation in longleaf pine survival across life stages, with lowest survival probabilities occurring during the bolt stage and not in the earlier, more fire-resistant grass stage. Survival of grass-stage, bolt-stage, and sapling longleaf pines was negatively associated with basal area of neighboring longleaf pine and positively related to neighboring heterospecific tree density, primarily oaks (Quercus spp.). Our findings highlight the vulnerability of longleaf pine across life stages, which suggests optimal fire management strategies for controlling longleaf pine density, and—more broadly—emphasize the importance of fire in mediating species interactions.
New Malolactic Bacteria Strains Isolated from Wine Microbiota: Characterization and Technological Properties
Authors: Filimon Răzvan Vasile , Bunea Claudiu-Ioan , Nechita Ancuța , Bora Florin Dumitru , Dunca Simona Isabela , Mocan Andrei , Filimon Roxana Mihaela
Published: January 2022

Malolactic fermentation (MLF) or biological decrease of wine acidity is defined as the enzymatic bioconversion of malic acid in lactic acid, a process performed by lactic acid bacteria (LAB). The procedures for the isolation of new indigenous LAB strains from the red wines produced in Copou Iasi wine center (NE of Romania) undergoing spontaneous malolactic fermentation, resulted in the obtaining of 67 catalase-negative and Gram-positive LAB strains. After testing in the malolactic fermentative process, application of specific screening procedures and identification (API 50 CH), two bacterial strains belonging to the species Oenococcus oeni (strain 13-7) and Lactobacillus plantarum (strain R1-1) with high yield of malolactic bioconversion, non-producing biogenic amines, and with active extracellular enzymes related to wine aroma, were retained and characterized. Tested in synthetic medium (MRS-TJ) for 10 days, the new isolated LAB strains metabolized over 98% of the malic acid at ethanol concentrations between 10 and 14 % (v/v), low pH (>3.0), total SO2 doses up to 70 mg/L and temperatures between 15 and 35 °C, showing high potential for future use in the winemaking process as bacterial starter cultures, in order to obtain high quality wines with increased typicity.
Characterization of Effective Diffusion within Viscoelastic Fluids with Elastic Instabilities
Authors: Hietsch Valerie , Ligrani Phil , Su Mengying
Published: January 2022

We considered effective diffusion, characterized by magnitudes of effective diffusion coefficients, in order to quantify mass transport due to the onset and development of elastic instabilities. Effective diffusion coefficient magnitudes were determined using different analytic approaches, as they were applied to tracked visualizations of fluorescein dye front variations, as circumferential advection was imposed upon a flow environment produced using a rotating Couette flow arrangement. Effective diffusion coefficient results were provided for a range of flow shear rates, which were produced using different Couette flow rotation speeds and two different flow environment fluid depths. To visualize the flow behavior within the rotating Couette flow environment, minute amounts of fluorescein dye were injected into the center of the flow container using a syringe pump. This dye was then redistributed within the flow by radial diffusion only when no disk rotation was used, and by radial diffusion and by circumferential advection when disk rotation was present. Associated effective diffusion coefficient values, for the latter arrangement, were compared to coefficients values with no disk rotation, which were due to molecular diffusion alone, in order to quantify enhancements due to elastic instabilities. Experiments were conducted using viscoelastic fluids, which were based on a 65% sucrose solution, with different polymer concentrations ranging from 0 ppm to 300 ppm. Associated Reynolds numbers based on the fluid depth and radially averaged maximum flow velocity ranged from 0.00 to 0.5. The resulting effective diffusion coefficient values for different flow shear rates and polymer concentrations quantified the onset of elastic instabilities, as well as significant and dramatic changes to local mass transport magnitudes, which are associated with the further development of elastic instabilities.
Effects of Processing on Starch Structure, Textural, and Digestive Property of “Horisenbada”, a Traditional Mongolian Food
Authors: Li Hongyan , Chen Zhijun , Mu Yifan , Ma Ruolan , Namujila Laxi , Fu Minghai
Published: January 2022

Horisenbada, prepared by the soaking, steaming, and baking of millets, is a traditional Mongolian food and is characterized by its long shelf life, convenience, and nutrition. In this study, the effect of processing on the starch structure, textural, and digestive property of millets was investigated. Compared to the soaking treatment, steaming and baking significantly reduced the molecular size and crystallinity of the millet starch, while baking increased the proportion of long amylose chains, partially destroyed starch granules, and formed a closely packed granular structure. Soaking and steaming significantly reduced the hardness of the millets, while the hardness of baked millets is comparable to that of raw millet grains. By fitting digestive curves with a first-order model and logarithm of the slope (LOS) plot, it showed that the baking treatment significantly reduced the digestibility of millets, the steaming treatment increased the digestibility of millets, while the soaked millets displayed a similar digestive property with raw millets, in terms of both digestion rate and digestion degree. This study could improve the understanding of the effects of processing on the palatability and health benefits of Horisenbada.
The Quality Changes and Proteomic Analysis of Cattle Muscle Postmortem during Rigor Mortis
Authors: Ding Zhenjiang , Wei Qichao , Liu Chunmei , Zhang Hong , Huang Feng
Published: January 2022

Rigor mortis occurs in a relatively early postmortem period and is a complex biochemical process in the conversion of muscle to meat. Understanding the quality changes and biomarkers during rigor mortis can provide a theoretical basis for maintaining and improving meat quality. Herein, a tandem mass tag proteomic method is used to investigate the effects of differentially expressed proteins on the meat quality of cattle Longissimus lumborum muscle postmortem (0, 6, and 24 h). The pH, total sulfhydryl content and sarcomere length decrease significantly during storage. In contrast, meat color values (L*, a*, and b*) and the myofibril fragmentation index increase significantly. Altogether, 147 differentially expressed proteins are identified, most being categorized as metabolic enzymes, mitochondrial proteins, necroptosis and ferroptosis proteins and structural proteins. The results also reveal additional proteins that are potentially involved in rigor mortis, such as cardiac phospholamban, acetyl-coenzyme A acyltransferase, and ankyrin repeat domain 2. The current results provide proteomic insights into the changes in meat quality during rigor mortis.