bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential Contribution of Anterior and Posterior Midcingulate Subregions to Distal and Proximal Threat Reactivity in Marmosets
Authors: Rahman Sufia S , Mulvihill Kevin , Wood Christian M , Quah Shaun K L , Horst Nicole K , Clarke Hannah F , Cockcroft Gemma J , Santangelo Andrea M , Roberts Angela C
Published: April 2021

The midcingulate cortex (MCC) is associated with cognition and emotion regulation. Structural and correlational functional evidence suggests that rather than being homogenous, the MCC may have dissociable functions that can be mapped onto distinct subregions. In this study, we use the marmoset monkey to causally investigate the contributions of two proposed subregions of the MCC: the anterior and posterior midcingulate cortices (aMCC and pMCC) to behavioral and cardiovascular correlates of threat processing relevant to anxiety disorders. Transient inactivation of the aMCC decreased anxiety-like responses to a postencounter distal threat, namely an unfamiliar human intruder, while inactivation of the pMCC showed a mild but opposing effect. Furthermore, although inactivation of neither MCC subregions had any effect on basal cardiovascular activity, aMCC inactivation blunted the expression of both cardiovascular and behavioral conditioned responses to a predictable proximal threat (a rubber snake) during the extinction in a Pavlovian conditioning task, with pMCC inactivation having again an opposing effect, but primarily on the behavioral response. These findings suggest that the MCC is indeed functionally heterogeneous with regards to its role in threat processing, with aMCC providing a marked facilitative contribution to the expression of the emotional response to both proximal and distal threat.
LC-PDA-MS/MS-Based Characterization of Key Phytochemicals in Eurycoma Longifolia Roots
Authors: Chua Lee Suan , Segaran Abirame , Wong Hoi Jin
Published: April 2021

The objective of the study was to fractionate the crude extract of Eurycoma longifolia (E. longifolia) roots and identify the intense peaks using HPLC-PDA-MS/MS, UPLC–MS/MS and H-NMR. Column chromatography was used to fractionate the crude extract into individual fractions using six solvent systems ranged from ethyl acetate, methanol and water in increasing polarity. Two fractions with nearly pure and intense peaks were selected for compound identification. Chromenone (coumarin) and chromone derivatives were putatively identified, besides several previously reported quassinoid glycosides (eurycomanone derived glycoside, 2,3-dehydro-4α-hydroxylongilactone glucoside, eurycomanol glycoside and eurycomanol trimer) in the fraction 11 of 100% methanol. A newly reported compound, namely hydroxyl glyyunanprosapogenin D (838 g/mol) was proposed to be the compound detected in the fraction 11 of 50% ethyl acetate and 50% methanol. This is also the first study to report the identification of chromenones and chromones in E. longifolia extract.
Potential Synergy Mechanism of Processing Methods for the Basic Remedies of Qixue Shuangbu Prescription Based on Integrated Metabolomics Strategy and Network Pharmacology Study
Authors: Chen Linwei , Sun Xiaojun , Wang Qin , Wu Wenjun , Tian Hu , Liu Lunyuan , Jiang Yong , Bei Chenqi , Chen Nan , Chen Jing
Published: April 2021

Qixue Shuangbu Prescription (QSP) is a famous traditional Chinese medicine (TCM) formula widely used for the treatment of chronic heart failure (CHF). Previous clinical studies have found that the efficacy of processed QSP has been significantly enhanced in the treatment of CHF. However, the synergistic mechanisms of processed QSP to enhance the treatment of CHF are still unclear. Generally, the changes in clinical effects mainly result from the variations of inside chemical basis caused by the TCM processing procedure. In this study, we developed a network pharmacology-integrated metabolomics strategy to clarify the difference of the effective compounds between crude and processed QSP, and further explain the mechanism of processed QSP to produce a synergistic effects. As a result, 69 different compounds were successfully screened, identified, quantified and verified as the most potential marker compounds. These different chemical components may play an anti-CHF and enhance the therapeutic effect through 52 action pathways such as estrogen signaling pathway, ubiquitin mediated proteolysis, protein processing in endoplasmic reticulum, etc. This study revealed that the proposed network pharmacology-integrated metabolomics strategy was a powerful tool for explaining the mechanism of synergistic action in the processing of QSP, further controlling the quality and understanding the processing mechanism of TCM formulae.
Prevalence of Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies Among Market and City Bus Depot Workers in Lima, Peru
Authors: Tovar Marco , Peinado Jesús , Palomino Santiago , Llanos Fernando , Ramírez Claudio , Valderrama Gisella , Calderón Roger I , Williams Roger B , Velásquez Gustavo E , Mitnick Carole D , Franke Molly F , Lecca Leonid
Published: April 2021

We report severe acute respiratory syndrome coronavirus 2 antibody positivity among market and city bus depot workers in Lima, Peru. Among 1285 vendors from 8 markets, prevalence ranged from 27% to 73%. Among 488 workers from 3 city bus depots, prevalence ranged from 11% to 47%. Self-reported symptoms were infrequent.
Safety and effectiveness of mucosal traction using a snare combined with endoclips to assist the resection of esophageal intraepithelial neoplasia: a propensity score matching analysis
Authors: Zhang Qiang , Lian Zhou-yang , Cai Jian-Qun , Bai Yang , Wang Zhen
Published: April 2021

Currently, the reports on esophageal endoscopic submucosal dissection (ESD) assisted by traction with a snare are rare. Because a snare is a commonly used endoscopic accessory and is easily available, its application in mucosal traction is worth exploring. The present study aims to evaluate the safety and effectiveness of snare-endoclip traction-assisted ESD for esophageal intraepithelial neoplasia. Cases of esophageal intraepithelial neoplasia resected using ESD in the Digestive Endoscopy Center of Guangzhou Nanfang Hospital, China from June 2013 to March 2019 were retrospectively analyzed. The procedure of snare-endoclip traction-assisted ESD was compared with nontraction-assisted ESD by using a propensity score matching analysis. Operation time, en bloc and R0 resection, intra- and postoperative complications, and surgery-related costs were mainly evaluated. Overall, 99 cases of esophageal intraepithelial neoplasia under tissue biopsy were included in the present study. Further, 22 exact matched pairs were obtained. There were no differences in en bloc and R0 resection rates, intra- and postoperative complications, and costs of disposable surgical accessories between the traction group and the nontraction group. However, median operation time showed a significant difference: traction group, 50.0 min (range, 20–100 min); nontraction group, 70.0 min (range, 35–133 min), P=0.012.

In conclusion, snare-endoclip traction-assisted ESD for esophageal intraepithelial neoplasia was safe and shortened operation time in the study, thereby improving the efficiency of ESD. Despite the additional use of a snare and endoclips for traction, the total costs of endoscopic accessories seemed not to be increased.
Laparoscopic transhiatal esophagectomy in esophageal cancer patients with high-risk post-operative complications
Authors: Takeda Flavio Roberto , Sallum Rubens Antonio Aissar , Ribeiro Jr Ulysses , Cecconello Ivan
Published: April 2021

Surgical treatment of esophageal cancer is challenging, due to considerable morbidity, especially in high surgical risk patients. While transhiatal esophagectomy leads to good oncological outcomes and reduced postoperative complications, less invasive techniques might further improve outcomes. Our goal was to compare results of laparoscopic transhiatal esophagectomy (LTE) with open transhiatal esophagectomy (OTE) in esophageal cancer patients at high surgical risk. From 2014 to 2020, 128 patients were identified. Seventy received OTE while 51 received LTE. After propensity score matching (1:1), postoperative complications, analysis of overall and disease-free survival, and survival-related prognostic factors were assessed in two groups of 48 patients. Ninety-one (77%) patients were men with a mean age of 65 ± 10.3 years. Those who underwent OTE experienced more clinical and surgical complications. In LTE patients, the number of mean resected lymph nodes was 25.9, and in patients who had OTE, it was 17.4 (P < 0.001). Overall survival was 56.0% in the LTE group and 33.6% (P = 0.023) in the OTE group. In multivariable analysis of overall survival, open surgery and incomplete pathological response were seen as worse negative factors. In multivariable analysis, metastatic lymph nodes, incomplete pathologic response, surgical complications, and a Charlson’s index > 2 (P = 0.014) were associated with poor prognosis. Both surgical methods are safe with similar morbidity and mortality; however, LTE was associated with fewer complications, a higher number of resected lymph nodes, better overall survival, and more prognostic factors related to global and disease-free overall survival in high-risk patients.
Biocontrol of Wireworms (Coleoptera: Elateridae) Using Entomopathogenic Nematodes: The Impact of Infected Host Cadaver Application and Soil Characteristics
Authors: Sandhi Ramandeep Kaur , Shapiro-Ilan David , Ivie Michael , Reddy Gadi V P
Published: April 2021

Wireworms have become a significant menace to cereals in the Northern Great Plains. Therefore, research toward developing effective control methods such as biological control with entomopathogenic nematodes (EPNs) is warranted. Two strains, each of two EPN species, Steinernema carpocapsae (Weiser) and Steinernema riobrave Cabanillas, Poinar, and Raulston in the form of infected Galleria mellonella (L.) cadavers were evaluated against wireworms in field and greenhouse. In field experiments, none of the four EPN strains were found effective against wireworms. However, in the greenhouse test, three of the strains, S. carpocapsae (All and Cxrd) or S. riobrave (355) applied in cadavers killed 50–68% of the sugarbeet wireworm, Limonius californicus (Mannerheim) was associated with 8–24% plant damage at 35 d after treatment (DAT), when seeds were treated with imidacloprid. The mortality range was 40–56% with 57–75% plant damage observed at 35 DAT, when seeds were planted without imidacloprid treatment. Synergistic effect among imidacloprid and S. carpocapsae (Cxrd) or S. riobrave (355) was observed in regard to L. californicus mortality. Additionally, effects of soil texture, moisture, and temperature on the infection rate of EPNs against L. californicus were examined in the laboratory. Limonius californicus mortality was not significantly affected by either soil moisture or soil types maintained at field capacity moisture levels. However, soil temperature showed a significant effect on L. californicus mortality. Overall, imidacloprid enhanced the infection and killing ability of EPNs against L. californicus and S. carpocapsae (All and Cxrd) strains were the virulent strains in different soil experiments.
Determination of the geopotential value on the permanent GNSS stations in Vietnam based on the Geodetic Boundary Value Problem approach
Authors: Vu Dinh Toan , Bruinsma Sean , Bonvalot Sylvain , Bui Luyen K , Balmino Georges
Published: April 2021

In the realisation of the International Height Reference System, the determination of the geopotential value and its variations in time plays an important role. In this study, the geodetic boundary value problem approach is applied for direct determination of the gravity potential value using a GOCE global gravity field model enhanced with terrestrial gravity data. This determination is carried out on the Global Navigation Satellite System-Continuously Operating Reference Stations (GNSS-CORS) stations towards the realisation of the International Height Reference System in Vietnam. First, the effects of the GOCE global gravity field model omission error, the indirect bias term on the disturbing potential and the systematic cumulative errors in levelling data are estimated. These errors affect the estimated geopotential value. The results calculated on the GNSS/levelling points show that the effect of the GOCE DIR-R5 (up to degree/order 260) omission error on the offset potential value is quite significant. This effect was eliminated using high-resolution terrestrial gravity data using the remove-compute-restore technique. The indirect bias term on the disturbing potential can be safely neglected by using a GOCE global gravity field model for degrees higher than 60 for this study region. The systematic cumulative errors in levelling data can be modelled and removed using a third-order polynomial model. Then, the mean zero-height gravity potential of the Vietnam local vertical datum is estimated equal to ${\rm{W}}_0^{{\rm{LVD}}}$ = 62 636 846.69 m2 s–2 with standard deviation of 0.70 m2 s–2 based on the proposed approach. Finally, the geodetic boundary value problem approach was used to determine the geopotential on the surface of three GNSS-CORS stations in Vietnam. Based on time-series of the vertical component derived from the GNSS observations as well as InSAR data, temporal variations in geopotential are also estimated on these permanent GNSS stations. The purpose is to monitor deformation of the vertical datum. The results indicate that the geopotential value needs to be monitored and determined with the time-dependent component on the three Vietnamese permanent GNSS stations for a vertical datum. These stations may contribute to increase the density of reference points in the International Terrestrial Reference Frame, which is being researched and implemented by the International Association of Geodesy.
A health and wellbeing programme for preadolescents in underserved Australian communities: child and stakeholder perspectives
Authors: Norman Jennifer , van Weerdenburg Katherine , Furber Susan , Stratten Maddison , Okely Anthony D
Published: April 2021

Social inequities in childhood affect children’s health and development. Active In-Betweens is a weekly, healthy lifestyle and outdoor activity after-school programme, tailor-made for preadolescent children (9–12 year olds), incorporating a strengths-based, trauma-informed, co-designed and place-based approach. This study evaluated the extent to which the programme strategies and activities met the anticipated short- to medium-term programme outcomes during its first year of operation in two socio-economically disadvantaged housing communities in New South Wales, Australia. A qualitative case study used data from semi-structured interviews with child participants (n = 11) and key stakeholders (n = 10). A broad range of positive outcomes were described. Children’s feedback indicated they had experienced opportunities which facilitated the development of new physical activity skills; new healthy eating experiences and knowledge; positive relationships with peers and facilitators and new connections with neighbourhood programmes and outdoor environments. Stakeholders valued the programme for the positive outcomes they observed among the children and the strong organizational partnerships which resulted. The importance of skilled facilitators to deliver the programme, the engagement of local stakeholders and a long-term commitment to programme delivery, with secure funding to ensure continuity, were clearly identified as integral for effective, sustainable outcomes.
Racing Pulses: Gender, Professionalism and Health Care in Medical Romance Fiction
Authors: Arnold-Forster Agnes
Published: April 2021

Following the foundation of the NHS in 1948, a new sub-genre of romantic fiction emerged: ‘Doctor–Nurse’ romances, usually involving romance between a male doctor and a female nurse, were set in NHS hospitals. Drawing on the Mills & Boon archive and the novels themselves, this article explores representations of the health service and notions of gendered healthcare professionalism in postwar Britain. I argue that rather than presenting ‘retrograde’ and ‘limited’ views of women’s lives, medical Mills & Boon novels frequently put forward nuanced versions of womanhood, professional identity, clinical labour, and the effective functioning of the welfare state.
Most Dogs Are Not NATIVE Dogs
Authors: Koster Jeremy
Published: April 2021

Studies of dogs have proliferated among canine scientists, aided in part by the logistical convenience of working with owned animals whose care is handled by others. These pet dogs are unlike most dogs that have lived in contemporary or prehistoric settings. In particular, many of the dogs studied by canine scientists are NATIVE dogs: (1) neutered, (2) alimented, (3) trained, (4) isolated, (5) vaccinated, and (6) engineered. The distinct genotypes and unusual environments of NATIVE dogs stand in contrast to the characteristics of dogs who have adapted to lives in other human communities and settings. For a holistic perspective on the evolution of dogs, it is helpful to study dogs in environments that share features of the settings in which dogs evolved.
Intraprocedural arrhythmia termination as an end point for hybrid ablation in patients with long-standing persistent atrial fibrillation: a 2-year follow-up study
Authors: Tan Chen , Zeng Li-Jun , Shi Hai-Feng , Tian Ying , Ma Nan , Liu Hao , Li Sheng-Chao , Hu Xue-Hong , Mei Ju , Liu Xing-Peng
Published: April 2021

OBJECTIVES

Hybrid catheter and surgical ablation has emerged as an effective therapy for patients with persistent atrial fibrillation (AF). The aims of this study were to evaluate the relationship between intraprocedural arrhythmia termination and the long-term outcomes of hybrid ablation in patients with long-standing persistent AF.

METHODS

From May 2015 through April 2019, 50 patients with persistent AF with a mean duration of 73.3 ± 62.1 (median 54) months underwent single-step hybrid ablation. Pulmonary vein isolation, left atrial posterior wall isolation and left atrial appendage excision or closure were performed through a left-sided thoracoscopic approach. Subsequently, all patients underwent high-density endocardial mapping and electrogram-based ablation with the end point of AF termination.

RESULTS

We achieved intraprocedural AF termination in 84% (42/50) patients; this end point was reached in 16 patients during surgical ablation and in 26 patients during catheter ablation. Seven patients underwent repeat catheter ablation. After a mean follow-up period of 29 ± 13 months, the freedom from atrial tachyarrhythmia of a single procedure without the use of antiarrhythmic drugs was 70% (35/50). In the Cox regression model, intraprocedural termination of AF (hazard ratio 0.205, 95% confidence interval 0.058–0.730; P = 0.014) was the sole predictor of success.

CONCLUSIONS

The 2-year outcomes of a one-stop hybrid ablation with an end point of AF termination are promising in patients with long-standing persistent AF.
Cohomology Groups for Spaces of Twelve-Fold Tilings
Authors: Bédaride Nicolas , Gähler Franz , Lecuona Ana G
Published: April 2021

We consider tilings of the plane with twelve-fold symmetry obtained by the cut-and-projection method. We compute their cohomology groups using the techniques introduced in [ 9]. To do this, we completely describe the window, the orbits of lines under the group action, and the orbits of 0-singularities. The complete family of generalized twelve-fold tilings can be described using two-parameters and it presents a surprisingly rich cohomological structure. To put this finding into perspective, one should compare our results with the cohomology of the generalized five-fold tilings (more commonly known as generalized Penrose tilings). In this case, the tilings form a one-parameter family, which fits in simply one of the two types of cohomology.
Introduction, Transmission Dynamics, and Fate of Early Severe Acute Respiratory Syndrome Coronavirus 2 Lineages in Santa Clara County, California
Authors: Villarino Elsa , Deng Xianding , Kemper Carol A , Jorden Michelle A , Bonin Brandon , Rudman Sarah L , Han George S , Yu Guixia , Wang Candace , Federman Scot , Bushnell Brian ,   CZB COVIDTracker Consortium, Wadford Debra A , Lin Wen , Tao Ying , Paden Clinton R , Bhatnagar Julu , MacCannell Tara , Tong Suxiang , Batson Joshua , Chiu Charles Y
Published: April 2021

We combined viral genome sequencing with contact tracing to investigate introduction and evolution of severe acute respiratory syndrome coronavirus 2 lineages in Santa Clara County, California, from 27 January to 21 March 2020. From 558 persons with coronavirus disease 2019, 101 genomes from 143 available clinical samples comprised 17 lineages, including SCC1 (n = 41), WA1 (n = 9; including the first 2 reported deaths in the United States, with postmortem diagnosis), D614G (n = 4), ancestral Wuhan Hu-1 (n = 21), and 13 others (n = 26). Public health intervention may have curtailed the persistence of lineages that appeared transiently during February and March. By August, only D614G lineages introduced after 21 March were circulating in Santa Clara County.
Immediate Bacille Calmette-Guérin Vaccination to Neonates Requiring Perinatal Treatment at the Maternity Ward in Guinea-Bissau: A Randomized Controlled Trial
Authors: Schaltz-Buchholzer Frederik , Aaby Peter , Monteiro Ivan , Camala Luis , Faurholt Simonsen Simone , Nørtoft Frankel Hannah , Lindberg Larsen Kristina , Golding Christian N , Kollmann Tobias R , Amenyogbe Nelly , Stabell Benn Christine , Bjerregaard-Andersen Morten
Published: April 2021

Background

Randomized controlled trials (RCTs) indicate that bacille Calmette-Guérin (BCG) vaccination provides broad beneficial “nonspecific” protection against infections. We investigated the effect on in-hospital mortality of providing BCG immediately upon admission to a neonatal intensive care unit (NICU), rather than BCG-at-discharge. The pretrial NICU mortality was 13% and we hypothesized that BCG would reduce mortality by 40%.

Methods

Parallel-group, open-label RCT was initiated in 2013 in Guinea-Bissau. Neonatal intensive care unit-admitted neonates were randomized 1:1 to BCG + oral polio vaccine (OPV) immediately (intervention) versus BCG + OPV at hospital discharge (control; usual practice). The trial was discontinued due to decreasing in-hospital mortality and major NICU restructuring. We assessed overall and disease-specific mortality by randomization allocation in cox proportional hazards models providing mortality rate ratios (MRRs).

Results

We recruited 3353 neonates, and the overall mortality was 3.1% (52 of 1676) for BCG-vaccinated neonates versus 3.3% (55 of 1677) for controls (MRR = 0.94; 0.64–1.36). For noninfectious causes of death, the MRR was 1.20 (0.70–2.07), and there tended to be fewer deaths from infections in the BCG group (N = 14) than among controls (N = 21) (MRR = 0.65; 0.33–1.28).

Conclusions

Providing BCG + OPV to frail neonates was safe and might protect against fatal infection in the immediate newborn period. Deaths due to prematurity and perinatal complications were unaffected by BCG.
In vitro activity of ceftazidime/avibactam against clinical isolates of Enterobacterales and Pseudomonas aeruginosa from Middle Eastern and African countries: ATLAS global surveillance programme 2015–18
Authors: Karlowsky James A , Bouchillon Samuel K , El Mahdy Kotb Ramy , Mohamed Naglaa , Stone Gregory G , Sahm Daniel F
Published: April 2021

Objectives

To assess the in vitro activity of ceftazidime/avibactam against a recent, 2015–18, collection of clinical isolates of Gram-negative bacilli from Middle Eastern and African countries with a focus on isolates from ICUs and with MDR and difficult-to-treat resistance (DTR) phenotypes.

Methods

Antimicrobial susceptibility testing of 4608 isolates of Enterobacterales (997 isolates from ICU patients) and 1358 isolates of Pseudomonas aeruginosa (374 isolates from ICU patients) was performed by CLSI broth microdilution methodology in a central laboratory. MICs were interpreted using both CLSI (2020) and EUCAST (2020) MIC breakpoints.

Results

Most isolates of Enterobacterales (Middle East: ICU, 99.1% susceptible, non-ICU, 99.1%; Africa: ICU, 96.9% susceptible, non-ICU, 98.3%) and P. aeruginosa (Middle East: ICU, 93.4%, non-ICU, 92.1%; Africa: ICU, 89.8%; non-ICU, 94.1%) were susceptible to ceftazidime/avibactam. Applying CLSI and EUCAST breakpoints, MDR rates were similar for Enterobacterales (27.8%–36.0% of isolates) and P. aeruginosa (25.0%–36.4%) while DTR rates were lower for Enterobacterales (1.6%–1.8%) than for P. aeruginosa (5.2%–7.4%). Percentage susceptible rates for ceftazidime/avibactam for MDR Enterobacterales were 96.8%–97.5% (Middle East) and 92.5%–94.3% (Africa) while rates for P. aeruginosa were 70.1%–80.0% (Middle East) and 69.5%–78.2% (Africa). 60.5%–65.8% (Middle East) and 38.9%–52.2% (Africa) of isolates of Enterobacterales with DTR phenotypes were ceftazidime/avibactam susceptible as were 29.2%–31.1% (Middle East) and 28.2%–35.8% (Africa) of DTR P. aeruginosa.

Conclusions

Overall, the isolates of Enterobacterales and P. aeruginosa tested from Middle Eastern and African countries were highly susceptible to ceftazidime/avibactam. Most MDR and many DTR isolates of Enterobacterales and P. aeruginosa were susceptible to ceftazidime/avibactam.
Management of Pea Leaf Weevil (Coleoptera: Curculionidae) and Development of a Nominal Threshold in Faba Beans
Authors: Wijerathna Asha , Evenden Maya , Reid Patty , Tidemann Breanne , Cárcamo Héctor
Published: April 2021

Pea leaf weevil, Sitona lineatus (L.) (Coleoptera: Curculionidae), can reduce the yield of field pea [Pisum sativum (L.) (Fabales: Fabaceae)] and faba bean [Vicia faba (L.) (Fabales: Fabaceae)]. Adults feed on the foliage and larvae feed on root nodules and nodule-associated Rhizobium Frank (Rhizobiales: Rhizobiacea) bacteria. In this study, we developed a data-based nominal threshold for pea leaf weevil in faba bean. We further tested the efficacy of insecticidal seed treatment and foliar insecticide (thiamethoxam and lambda-cyhalothrin, respectively), and nitrogen amendment for pea leaf weevil control using a multi-year field plot study at two sites in Alberta, Canada. Pea leaf weevil feeding damage significantly reduced faba bean yields. Thiamethoxam reduced adult and larval damage, and protected faba bean yield, while neither lambda-cyhalothrin nor a nitrogen amendment was effective in protecting yield. The percentage of seedlings with feeding on the terminal leaf had a negative relationship with yield and was used to estimate a nominal threshold near 15% of seedlings with terminal leaf damage. Since lambda-cyhalothrin is not effective in managing pea leaf weevil on faba bean, there is a need to research additional integrated pest management strategies to reduce prophylactic insecticidal seed treatments.
Partial Budget Analysis of Exclusion Netting and Organic-certified Insecticides for Management of Spotted-wing Drosophila (Diptera: Drosophilidae) on Small Farms in the Upper Midwest
Authors: DiGiacomo G , Gullickson M G , Rogers M , Peterson H H , Hutchison W D
Published: April 2021

Drosophila suzukii Matsumura (Diptera: Drosophilidae), or spotted-wing drosophila, is an invasive pest first detected in the United States in 2008. Although D. suzukii can use many cultivated fruit as hosts, raspberries are considered ‘most at risk’ for infestation. Conventional broad-spectrum insecticides are proven effective D. suzukii controls and can be economically profitable when combined with integrated pest management (IPM) on large-scale commercial raspberry farms. It remains unclear, however, whether organic controls are cost-effective strategies, particularly for farms operating on a small-scale seasonal basis, as is common in the Upper Midwest. The purpose of this paper is to explore the efficacy of two organic D. suzukii controls—exclusion netting for high tunnels and organic insecticides for open plots using data available from different field trials—and to ascertain whether any economic benefits of the organic controls outweigh treatment costs for small-scale raspberry operations under different risk scenarios. The field trials suggest that the organic treatments are effective controls for D. suzukii infestation and economically profitable. The exclusion netting treatment produced positive net returns compared to the alternative of no treatment and economically outperformed the organic-certified insecticide treatment for several yield, price and infestation scenarios. As D. suzukii infestation rates increased, net returns improved for both organic treatments. The economic results were robust across a range of yields and prices, suggesting that in almost all scenarios small scale organic raspberry growers benefit economically from the application of exclusion netting on high tunnels and insecticides for open plots.
Treatment of liver cancer cells with ethyl acetate extract of Crithmum maritimum permits reducing sorafenib dose and toxicity maintaining its efficacy
Authors: Gnocchi Davide , Castellaneta Francesca , Cesari Gianluigi , Fiore Giorgio , Sabbà Carlo , Mazzocca Antonio
Published: April 2021

Objectives

Hepatocellular carcinoma (HCC) is one of the most frequent tumours worldwide and available drugs are inadequate for therapeutic results and tolerability. Hence, novel effective therapeutic tools with fewer side effects are of paramount importance. We have previously shown that Crithmum maritimum ethyl acetate extract exerts a cytostatic effect in HCC cells. Here, we tested whether C. maritimum ethyl acetate extract in combination with half sorafenib IC50 dose ameliorated efficacy and toxicity of sorafenib in inhibiting liver cancer cell growth. Moreover, we investigated the mechanisms involved.

Methods

Two HCC cell lines (Huh7 and HepG2) were treated with C. maritimum ethyl acetate extract and half IC50 sorafenib dose usually employed in vitro. Then, cell proliferation, growth kinetics and cell toxicity were analysed together with an investigation of the cellular mechanisms involved, focusing on cell cycle regulation and apoptosis.

Key findings

Results show that combined treatment with C. maritimum ethyl acetate extract and half IC50 sorafenib dose decreased cell proliferation comparably to full-dose sorafenib without increasing cell toxicity as confirmed by the effect on cell cycle regulation and apoptosis.

Conclusions

These results provide scientific support for the possibility of an effective integrative therapeutic approach for HCC with fewer side effects on patients.
Liver Blood Tests in the Management of Suspected Choledocholithiasis
Authors: Mongelli Francesco , Di Giuseppe Matteo , Porcellini Iride , Proietti Francesco , Cristaudi Alessandra , Pini Ramon , La Regina Davide
Published: April 2021

Objective

The likelihood of common bile duct (CBD) stones considers liver blood tests (LBTs) if they are markedly altered only. The aim of our study was to find a reliable tool based on LBTs to predict the presence of CBD stones.

Methods

We retrospectively considered all patients who underwent magnetic resonance cholangiopancreatography (MRCP) because of suspected CBD stones from January 2014 to June 2019. Demographic, clinical data, and LBT values were collected and analyzed.

Results

We selected 191 patients, 64 (33.5%) with positive MRCP and 127 (66.5%) with negative MRCP. The analysis showed that our compound LBT-based score had 83.6%, 90.7%, and 90.6% sensitivity, specificity, and negative predictive values, respectively, in determining MRCP results.

Conclusion

We designed a weighted score with high diagnostic power in determining MRCP results that could help in differentiating between candidates for primary cholecystectomy and patients who benefit from preoperative MRCP.
EDGE: two routes to dark matter core formation in ultra-faint dwarfs
Authors: Orkney Matthew D A , Read Justin I , Rey Martin P , Nasim Imran , Pontzen Andrew , Agertz Oscar , Kim Stacy Y , Delorme Maxime , Dehnen Walter
Published: April 2021

In the standard Lambda cold dark matter paradigm, pure dark matter simulations predict dwarf galaxies should inhabit dark matter haloes with a centrally diverging density ‘cusp’. This is in conflict with observations that typically favour a constant density ‘core’. We investigate this ‘cusp-core problem’ in ‘ultra-faint’ dwarf galaxies simulated as part of the ‘Engineering Dwarfs at Galaxy formation’s Edge’ project. We find, similarly to previous work, that gravitational potential fluctuations within the central region of the simulated dwarfs kinematically heat the dark matter particles, lowering the dwarfs’ central dark matter density. However, these fluctuations are not exclusively caused by gas inflow/outflow, but also by impulsive heating from minor mergers. We use the genetic modification approach on one of our dwarf’s initial conditions to show how a delayed assembly history leads to more late minor mergers and, correspondingly, more dark matter heating. This provides a mechanism by which even ultra-faint dwarfs ($M_* \lt 10^5\, \text{M}_{\odot }$), in which star formation was fully quenched at high redshift, can have their central dark matter density lowered over time. In contrast, we find that late major mergers can regenerate a central dark matter cusp, if the merging galaxy had sufficiently little star formation. The combination of these effects leads us to predict significant stochasticity in the central dark matter density slopes of the smallest dwarfs, driven by their unique star formation and mass assembly histories.
OH in the diffuse interstellar medium: physical modelling and prospects with upcoming SKA precursor/pathfinder surveys
Authors: Balashev S A , Gupta N , Kosenko D N
Published: April 2021

Hydroxyl ($\rm OH$) is known to form efficiently in cold gas (T ∼ 100 K) along with the molecule $\rm H_2$ and can be used as an efficient tracer of the diffuse molecular gas in the interstellar medium (ISM). Using a simple formalism describing the $\rm H\, I/H_2$ transition and a reduced network of major chemical reactions, we present a semi-analytical prescription to estimate the abundances of O-bearing molecules in the diffuse ISM. We show that predictions based on our prescription are in good agreement with the estimates obtained using the meudon pdr code which utilizes the full reaction network. We investigate the dependence of the relative abundances of $\rm OH/H\, I$ and $\rm OH/H_2$ on the variations of physical conditions i.e. the metallicity, number density (n), cosmic ray ionization rate (ζ), and strength of UV field (χ) in the medium. We find that the $\rm OH/H\, I$ abundances observed in the Galactic ISM can be reproduced by models with n ∼ 50 cm−3, χ ∼ 1 (Mathis field), and ζ ∼ 3 × 10−17 s−1, with a variation of about 1 dex allowed around these values. Using the constrained $\rm H_2$ column density distribution function at z ∼ 3, we estimate the $\rm OH$ column density distribution function and discuss future prospects with the upcoming large radio absorption line surveys.
A Case of Gene Fragmentation in Plant Mitochondria Fixed by the Selection of a Compensatory Restorer of Fertility-Like PPR Gene
Authors: Nguyen Tan-Trung , Planchard Noelya , Dahan Jennifer , Arnal Nadège , Balzergue Sandrine , Benamar Abdelilah , Bertin Pierre , Brunaud Véronique , Dargel-Graffin Céline , Macherel David , Martin-Magniette Marie-Laure , Quadrado Martine , Namy Olivier , Mireau Hakim
Published: April 2021

The high mutational load of mitochondrial genomes combined with their uniparental inheritance and high polyploidy favors the maintenance of deleterious mutations within populations. How cells compose and adapt to the accumulation of disadvantageous mitochondrial alleles remains unclear. Most harmful changes are likely corrected by purifying selection, however, the intimate collaboration between mitochondria- and nuclear-encoded gene products offers theoretical potential for compensatory adaptive changes. In plants, cytoplasmic male sterilities are known examples of nucleo-mitochondrial coadaptation situations in which nuclear-encoded restorer of fertility (Rf) genes evolve to counteract the effect of mitochondria-encoded cytoplasmic male sterility (CMS) genes and restore fertility. Most cloned Rfs belong to a small monophyletic group, comprising 26 pentatricopeptide repeat genes in Arabidopsis, called Rf-like (RFL). In this analysis, we explored the functional diversity of RFL genes in Arabidopsis and found that the RFL8 gene is not related to CMS suppression but essential for plant embryo development. In vitro-rescued rfl8 plantlets are deficient in the production of the mitochondrial heme–lyase complex. A complete ensemble of molecular and genetic analyses allowed us to demonstrate that the RFL8 gene has been selected to permit the translation of the mitochondrial ccmFN2 gene encoding a heme–lyase complex subunit which derives from the split of the ccmFN gene, specifically in Brassicaceae plants. This study represents thus a clear case of nuclear compensation to a lineage-specific mitochondrial genomic rearrangement in plants and demonstrates that RFL genes can be selected in response to other mitochondrial deviancies than CMS suppression.
Excess fibronectin 1 participates in pathogenesis of pre-eclampsia by promoting apoptosis and autophagy in vascular endothelial cells
Authors: Wu Haiying , Liu Kan , Zhang Jingli
Published: April 2021

Plasma fibronectin 1 (FN1) levels are elevated in individuals with pre-eclampsia (PE), which may be applied as a possible b marker for vascular endothelial injury during PE. In the present study, the possible role of FN1 in the pathogenesis of PE and regulation of apoptosis and autophagy in vascular endothelial cells was explored. Plasma FN1 levels in 80 patients with PE and 40 healthy pregnant individuals were measured using ELISA to verify its relationship with the severity of PE. pcDNA3.1-FN1 or FN1-small interfering (si) RNA was used to manipulate the expression of FN1 in human umbilical vein endothelial cells (HUVECs) to assess the effects of FN1 on cell apoptosis, autophagy, and the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mechanistic target of rapamycin (mTOR) signaling pathway. It was found that upregulation of FN1 promoted apoptosis and autophagy, in addition to significantly inhibiting the activation of AKT and mTOR in HUVECs. By contrast, downregulation of FN1 expression inhibited cell apoptosis and autophagy, but increased AKT and mTOR phosphorylation in HUVECs that were cultured in serum samples obtained from patients with PE. Rescue experiments found that the PI3K/AKT inhibitor LY294002 reversed the effects of FN1-siRNA on apoptosis and autophagy in HUVECs cultured in serum from patients with PE. Therefore, data from the present study suggest that FN1 participates in the pathogenesis of PE by promoting apoptosis and autophagy in vascular endothelial cells, which is associated with the PI3K/AKT/mTOR signaling pathway.
Activation-induced deaminase is critical for the establishment of DNA methylation patterns prior to the germinal center reaction
Authors: Català-Moll Francesc , Ferreté-Bonastre Anna G , Li Tianlu , Weichenhan Dieter , Lutsik Pavlo , Ciudad Laura , Álvarez-Prado Ángel F , Rodríguez-Ubreva Javier , Klemann Christian , Speckmann Carsten , Vilas-Zornoza Amaya , Abolhassani Hassan , Martínez-Gallo Mónica , Dieli-Crimi Romina , Rivière Jacques G , Martín-Nalda Andrea , Colobran Roger , Soler-Palacín Pere , Kracker Sven , Hammarström Lennart , Prosper Felipe , Durandy Anne , Grimbacher Bodo , Plass Christoph , Ballestar Esteban
Published: April 2021

Activation-induced deaminase (AID) initiates antibody diversification in germinal center B cells by deaminating cytosines, leading to somatic hypermutation and class-switch recombination. Loss-of-function mutations in AID lead to hyper-IgM syndrome type 2 (HIGM2), a rare human primary antibody deficiency. AID-mediated deamination has been proposed as leading to active demethylation of 5-methycytosines in the DNA, although evidence both supports and casts doubt on such a role. In this study, using whole-genome bisulfite sequencing of HIGM2 B cells, we investigated direct AID involvement in active DNA demethylation. HIGM2 naïve and memory B cells both display widespread DNA methylation alterations, of which ∼25% are attributable to active DNA demethylation. For genes that undergo active demethylation that is impaired in HIGM2 individuals, our analysis indicates that AID is not directly involved. We demonstrate that the widespread alterations in the DNA methylation and expression profiles of HIGM2 naïve B cells result from premature overstimulation of the B-cell receptor prior to the germinal center reaction. Our data support a role for AID in B cell central tolerance in preventing the expansion of autoreactive cell clones, affecting the correct establishment of DNA methylation patterns.
Effectiveness of Mobilization of the Talus and Distal Fibula in the Management of Acute Lateral Ankle Sprain
Authors: Izaola-Azkona Loitzun , Vicenzino Bill , Olabarrieta-Eguia Iratxe , Saez Marc , Lascurain-Aguirrebeña Ion
Published: April 2021

Objective

Distal fibular mobilization with movement (MWM), with and without a posterior gliding fibular tape, and anteroposterior mobilization of the talus (MOB) are widely used to treat acute lateral ankle sprains. The purpose of this study was to investigate the short-term and long-term relative effectiveness of these techniques.

Methods

In this double-blind randomized controlled trial, 45 amateur soccer players with acute (<72 hours) lateral ankle sprain were randomly allocated to 6 sessions (3/wk within the first 2 weeks) of either MWM, MWM with tape (MWMtape), or MOB. All participants also received general advice, transcutaneous electrical nerve stimulation, edema draining massage, and a program of proprioception exercises. Participant ratings of function on the Foot and Ankle Ability Measure and Patient Global Impression of Improvement Scale were the primary outcomes measured over 52 weeks. Secondary outcomes were ankle pain, pressure pain threshold, range of motion, volume, and strength.

Results

MWM and MWMtape were equally effective and participants demonstrated greater function on the Foot and Ankle Ability Measure at 12 and 52 weeks when compared with those receiving MOB; however, the latter demonstrated superior function at 2 weeks. No differences between groups were observed for Patient Global Impression of Improvement Scale or any of the secondary outcomes.

Conclusion

There are limited differences in the short term among techniques, with the exception of better sport function with MOB. Over the longer term, the distal fibular MWM is most effective to achieve activities of daily living and sport function when added to usual physical therapy care. The addition of a posterior gliding fibular tape provides no additional benefit.

Impact

Distal fibular mobilization with movement may be the most appropriate choice of treatment for acute lateral ankle sprain to achieve long-term activities of daily living and sport function. In the short term, anteroposterior mobilization of the talus offers greater improvement in sport function. The use of fibular tape provides no added benefit as an adjunct to a treatment that includes distal fibular mobilization with movement.
Public Procurement, Regional Integration, and the Belt and Road Initiative
Authors: Ghossein Tania , Hoekman Bernard , Shingal Anirudh
Published: April 2021

China's Belt and Road Initiative (BRI) is a mechanism through which countries can upgrade connectivity-related infrastructure, including through cross-border projects, complementing traditional sources of finance. An overarching goal of the BRI is to reduce trade costs between China and partner countries, in part by helping to integrate regional markets. The large-scale borrowing associated with BRI projects has given rise to potential debt servicing and sustainability concerns. The rate of return of BRI regional infrastructure projects depends in part on the integrity of public procurement processes and realizing value-for-money objectives. To date BRI projects financed by Chinese institutions have been largely awarded to Chinese companies. Enhancing transparency of BRI procurement processes and international cooperation among countries participating in the BRI would help achieve value for money goals and support the integration of BRI countries.
Surveillance of Depleted Uranium-exposed Gulf War Veterans: More Evidence for Bone Effects
Authors: McDiarmid Melissa A. , Gaitens Joanna M. , Hines Stella , Cloeren Marianne , Breyer Richard , Condon Marian , Oliver Marc , Roth Tracy , Gucer Patricia , Kaup Bruce , Brown Lawrence , Brown Clayton H. , Dux Moira , Glick Danielle , Lewin-Smith Michael R. , Strathmann Frederick , Xu Hanna , Velez-Quinones Maria A. , Streeten Elizabeth
Published: April 2021

Gulf War I veterans who were victims of depleted uranium (DU) “friendly-fire” incidents have undergone longitudinal health surveillance since 1994. During the spring of 2019, 36 members of the cohort were evaluated with a monitoring protocol including exposure assessment for total and isotopic uranium concentrations in urine and a comprehensive review of health outcomes, including measures of bone metabolism and bone mineral density (BMD) determination. Elevated urine U concentrations were observed in cohort members with retained depleted uranium (DU) shrapnel fragments. In addition, a measure of bone resorption, N-telopeptide, showed a statistically significant increase in those in the high DU subgroup, a finding consistent with a statistically significant decrease in bone mass also observed in this high DU subgroup compared to the low DU subgroup. After more than 25 y since first exposure to DU, an aging cohort of military veterans continues to show few U-related health effects in known target organs of U toxicity. The new finding of impaired BMD in the high DU subgroup has now been detected in two consecutive surveillance visits. While this is a biologically plausible uranium effect, it is not reflected in other measures of bone metabolism in the full cohort, which have largely been within normal limits. However, ongoing accrual of the U burden from fragment absorption over time and the effect of aging further impairing BMD suggest the need for future surveillance assessments of this cohort.
Maximum Possible Doses for a Cohort of Individuals with Intakes Possibly Containing a Component of a Ceramic-type Material
Authors: Klumpp John , Poudel Deepesh
Published: April 2021

Recently, a glovebox breach led to the potential exposure of 15 Los Alamos National Laboratory employees to 238Pu. Given what is known about the material involved in the incident, the possibility of an intake with a ceramic-type component must be considered. Incidents in which intakes of ceramic solubility-type material is suspected represent a challenge for internal dose assessment via urine bioassay because even relatively large doses cannot be detected in urine until many months after the intake. Ideally, in these situations fecal samples should be collected to assess the intake, but in this case fecal sampling was delayed. This paper presents a method to calculate the maximum possible doses for all individuals involved in an incident using only early time-decreasing urine bioassay measurements.