bluuebunny/crossref_metadata_2025 huggingface dataset:
N-Glycosylation in Piroplasmids: Diversity within Simplicity
Authors: Florin-Christensen Monica , Rodriguez Anabel E. , Suárez Carlos E. , Ueti Massaro W. , Delgado Fernando O. , Echaide Ignacio , Schnittger Leonhard
Published: January 2021

N-glycosylation has remained mostly unexplored in Piroplasmida, an order of tick-transmitted pathogens of veterinary and medical relevance. Analysis of 11 piroplasmid genomes revealed three distinct scenarios regarding N-glycosylation: Babesia sensu stricto (s.s.) species add one or two N-acetylglucosamine (NAcGlc) molecules to proteins; Theileria equi and Cytauxzoon felis add (NAcGlc)2-mannose, while B. microti and Theileria s.s. synthesize dolichol-P-P-NAcGlc and dolichol-P-P-(NAcGlc)2 without subsequent transfer to proteins. All piroplasmids possess the gene complement needed for the synthesis of the N-glycosylation substrates, dolichol-P and sugar nucleotides. The oligosaccharyl transferase of Babesia species, T. equi and C. felis, is predicted to be composed of only two subunits, STT3 and Ost1. Occurrence of short N-glycans in B. bovis merozoites was experimentally demonstrated by fluorescence microscopy using a NAcGlc-specific lectin. In vitro growth of B. bovis was significantly impaired by tunicamycin, an inhibitor of N-glycosylation, indicating a relevant role for N-glycosylation in this pathogen. Finally, genes coding for N-glycosylation enzymes and substrate biosynthesis are transcribed in B. bovis blood and tick stages, suggesting that this pathway is biologically relevant throughout the parasite life cycle. Elucidation of the role/s exerted by N-glycans will increase our understanding of these successful parasites, for which improved control measures are needed.
Metformin Improves Biochemical and Pathophysiological Changes in Hepatocellular Carcinoma with Pre-Existed Diabetes Mellitus Rats
Authors: Mobasher Maysa A. , Germoush Mousa O. , Galal El-Tantawi Hala , Samy El-Said Karim
Published: January 2021

Hepatocellular carcinoma (HCC) is one of the world’s most widely recognized malignant tumors that accounts for 90% of all the primary liver cancers and is a major cause of death from cancer, representing half a million deaths per year. Obesity and associated metabolic irregularities, particularly diabetes mellitus (DM) and insulin resistance, are important risk factors for the advancement of HCC. Recently, retrospective studies showed that metformin (MET) could protect the hepatic tissues in pre-existing diabetes mellitus from HCC. The purpose of this study was to assess the role of MET treatment in the pre-existing diabetic rats before and after HCC induction by diethylnitrosamine (DEN). Thirty-five male Sprague Dawley albino rats were partitioned into the following groups: Group 1 (Gp1) was the control. Gp2 was injected intraperitoneally (i.p) with streptozotocin (STZ) (80 mg/kg) and DEN (50 mg/kg/7 weeks). Gp3, Gp4, and Gp5 were injected as in Gp2 and treated with MET (150 mg/kg) before and/or after HCC induction. Biochemical parameters including liver functions, lipid profile, and oxidative stress biomarkers were determined. Furthermore, histological and immunohistochemical changes were assessed in all groups. Our results illustrated that the group of rats that were treated with STZ and DEN had significant changes in both liver functions and were associated with alterations in the liver histopathological architectures. Treatment with MET before or after HCC induction ameliorated the cellular changes in the liver tissues; however, the utmost protection was found in a group of rats, which were treated with MET before and after HCC induction.
Is an Immunosuppressive Microenvironment a Characteristic of Both Intra- and Extraparenchymal Central Nervous Tumors?
Authors: Soltani Amina , Kajtar Bela , Abdelwahab El Husseiny Mohamed Mahmud , Steib Anita , Horvath Zsolt , Mangel Laszlo , Jaromi Luca , Pongracz Judit E.
Published: January 2021

In spite of intensive research, the survival rates of patients diagnosed with tumors of the central nervous system (CNS) have not improved significantly in the last decade. Immunotherapy as novel and efficacious treatment option in several other malignancies has failed in neuro-oncology likely due to the immunosuppressive property of the brain tissues. Glioblastoma (GBM) is the most aggressive malignant CNS neoplasm, while meningioma (MNG) is a mainly low grade or benign brain tumor originating from the non-glial tissues of the CNS. The aim of the current preliminary study is to compare the immune microenvironment of MNG and GBM as potential target in immunotherapy. Interestingly, the immune microenvironment of MNG and GBM have proved to be similar. In both tumors types the immune suppressive elements including regulatory T cells (Treg), tumor-associated macrophages (TAM) were highly elevated. The cytokine environment supporting Treg differentiation and the presence of indoleamine 2,3-dioxygenase 1 (IDO1) have also increased the immunosuppressive microenvironment. The results of the present study show an immune suppressive microenvironment in both brain tumor types. In a follow-up study with a larger patient cohort can provide detailed background information on the immune status of individual patients and aid selection of the best immune checkpoint inhibitor or other immune modulatory therapy. Immune modulatory treatments in combination with IDO1 inhibitors might even become alternative therapy for relapsed, multiple and/or malignant MNG or chemo-resistant GBM.
Co-Clinical Trials: An Innovative Drug Development Platform for Cholangiocarcinoma
Authors: Balasubramanian Brinda , Venkatraman Simran , Myint Kyaw Zwar , Janvilisri Tavan , Wongprasert Kanokpan , Kumkate Supeecha , Bates David O. , Tohtong Rutaiwan
Published: January 2021

Cholangiocarcinoma (CCA), a group of malignancies that originate from the biliary tract, is associated with a high mortality rate and a concerning increase in worldwide incidence. In Thailand, where the incidence of CCA is the highest, the socioeconomic burden is severe. Yet, treatment options are limited, with surgical resection being the only form of treatment with curative intent. The current standard-of-care remains adjuvant and palliative chemotherapy which is ineffective in most patients. The overall survival rate is dismal, even after surgical resection and the tumor heterogeneity further complicates treatment. Together, this makes CCA a significant burden in Southeast Asia. For effective management of CCA, treatment must be tailored to each patient, individually, for which an assortment of targeted therapies must be available. Despite the increasing numbers of clinical studies in CCA, targeted therapy drugs rarely get approved for clinical use. In this review, we discuss the shortcomings of the conventional clinical trial process and propose the implementation of a novel concept, co-clinical trials to expedite drug development for CCA patients. In co-clinical trials, the preclinical studies and clinical trials are conducted simultaneously, thus enabling real-time data integration to accurately stratify and customize treatment for patients, individually. Hence, co-clinical trials are expected to improve the outcomes of clinical trials and consequently, encourage the approval of targeted therapy drugs. The increased availability of targeted therapy drugs for treatment is expected to facilitate the application of precision medicine in CCA.
The Role of Polyphenols in Abiotic Stress Response: The Influence of Molecular Structure
Authors: Šamec Dunja , Karalija Erna , Šola Ivana , Vujčić Bok Valerija , Salopek-Sondi Branka
Published: January 2021

Abiotic stressors such as extreme temperatures, drought, flood, light, salt, and heavy metals alter biological diversity and crop production worldwide. Therefore, it is important to know the mechanisms by which plants cope with stress conditions. Polyphenols, which are the largest group of plant-specialized metabolites, are generally recognized as molecules involved in stress protection in plants. This diverse group of metabolites contains various structures, from simple forms consisting of one aromatic ring to more complex ones consisting of large number of polymerized molecules. Consequently, all these molecules, depending on their structure, may show different roles in plant growth, development, and stress protection. In the present review, we aimed to summarize data on how different polyphenol structures influence their biological activity and their roles in abiotic stress responses. We focused our review on phenolic acids, flavonoids, stilbenoids, and lignans.
Short-Term Exposure to High Atmospheric Vapor Pressure Deficit (VPD) Severely Impacts Durum Wheat Carbon and Nitrogen Metabolism in the Absence of Edaphic Water Stress
Authors: Fakhet Dorra , Morales Fermín , Jauregui Iván , Erice Gorka , Aparicio-Tejo Pedro M. , González-Murua Carmen , Aroca Ricardo , Irigoyen Juan J. , Aranjuelo Iker
Published: January 2021

Low atmospheric relative humidity (RH) accompanied by elevated air temperature and decreased precipitation are environmental challenges that wheat production will face in future decades. These changes to the atmosphere are causing increases in air vapor pressure deficit (VPD) and low soil water availability during certain periods of the wheat-growing season. The main objective of this study was to analyze the physiological, metabolic, and transcriptional response of carbon (C) and nitrogen (N) metabolism of wheat (Triticum durum cv. Sula) to increases in VPD and soil water stress conditions, either alone or in combination. Plants were first grown in well-watered conditions and near-ambient temperature and RH in temperature-gradient greenhouses until anthesis, and they were then subjected to two different water regimes well-watered (WW) and water-stressed (WS), i.e., watered at 50% of the control for one week, followed by two VPD levels (low, 1.01/0.36 KPa and high, 2.27/0.62 KPa; day/night) for five additional days. Both VPD and soil water content had an important impact on water status and the plant physiological apparatus. While high VPD and water stress-induced stomatal closure affected photosynthetic rates, in the case of plants watered at 50%, high VPD also caused a direct impairment of the RuBisCO large subunit, RuBisCO activase and the electron transport rate. Regarding N metabolism, the gene expression, nitrite reductase (NIR) and transport levels detected in young leaves, as well as determinations of the δ15N and amino acid profiles (arginine, leucine, tryptophan, aspartic acid, and serine) indicated activation of N metabolism and final transport of nitrate to leaves and photosynthesizing cells. On the other hand, under low VPD conditions, a positive effect was only observed on gene expression related to the final step of nitrate supply to photosynthesizing cells, whereas the amount of 15N supplied to the roots that reached the leaves decreased. Such an effect would suggest an impaired N remobilization from other organs to young leaves under water stress conditions and low VPD.
Influence of Pre-Harvest Gibberellic Acid and Post-Harvest 1-methyl Cyclopropane Treatments on Phenolic Compounds, Vitamin C and Organic Acid Contents during the Shelf Life of Strawberry Fruits
Authors: Taş Akgül , Berk Selma Kuru , Orman Erdal , Gundogdu Muttalip , Ercişli Sezai , Karatas Neva , Jurikova Tunde , Adamkova Anna , Nedomova Sarka , Mlcek Jiri
Published: January 2021

In recent years, significant portions of the fresh fruits and vegetables produced worldwide have been decaying before reaching the consumer because of insufficient preservation after harvest. In this direction, we carried the study out to investigate the effect of gibberellic acid (GA3) and 1-methyl cyclopropane (1-MCP) applications on phenolic compounds and organic acid contents of the strawberry fruits (cv. Albion) during shelf-life. Gibberellic acid treatments, which prepared in two different concentrations (50 and 100 ppm), were performed by spraying the leaves before harvest. 1-methyl cyclopropane applied after harvest. The results of the study showed a greater decrease in organic acids (except oxalic and succinic acid) in Gibberellic acid-applied fruits during shelf-life. Citric acid was recorded as the most abundant organic acid in the control group. In phenolic compounds, gallic acid (15.22 mg 100 g−1) and ellagic acid (9.38 mg 100 g−1) were recorded as the highest phenolic compounds on the third day. 1-MCP and GA3 (50 ppm) + 1-MCP treatment reduced the breakdown of vitamin C during the shelf-life of strawberry fruits compared to the control group. As a result, phenolic compounds, vitamin C, and organic acids decreased during the shelf-life, and 1-MCP applications slowed down the breakdown of these compounds.
Assessment of Photo-Induced Cytotoxic Activity of Cachrys sicula and Cachrys libanotis Enriched-Coumarin Extracts against Human Melanoma Cells
Authors: Marrelli Mariangela , Perri Maria Rosaria , Amodeo Valentina , Giordano Francesca , Statti Giancarlo A. , Panno Maria Luisa , Conforti Filomena
Published: January 2021

Photochemotherapy is one of the most interesting current therapeutic approaches for the treatment of melanoma. Different classes of naturally occurring phytochemicals demonstrated interesting photoactive properties. The aim of this study was to evaluate the photocytotoxic potential of two Cachrys species from Southern Italy: C. sicula and C. libanotis (Apiaceae). The enriched-coumarin extracts were obtained from aerial parts through both traditional maceration and pressurized cyclic solid-liquid (PCSL) extraction using Naviglio extractor®. Qualitative and quantitative analyses of furanocoumarins were performed with GC-MS. The photocytotoxic effects were verified on C32 melanoma cells irradiated at a dose of 1.08 J/cm2. The apoptotic responses were also assessed. Moreover, phenolic content and the in vitro antioxidant potential were estimated. Xanthotoxin, bergapten, and isopimpinellin were identified. All the samples induced concentration-dependent photocytotoxic effects (IC50 ranging from 3.16 to 18.18 μg/mL). The C. libanotis sample obtained with Naviglio extractor® was the most effective one (IC50 = 3.16 ± 0.21 μg/mL), followed by C. sicula sample obtained with the same technique (IC50 = 8.83 ± 0.20 μg/mL). Both Cachrys samples obtained through PCSL induced up-regulation of apoptotic signals such as BAX (Bcl2-associated X protein) and PARP (poly ADP-ribose polymerase) cleavage. Moreover, these samples proved to be more photoactive, giving a greater upregulation of p21 protein in the presence of UVA radiation. Obtained results suggest that investigated species could be promising candidates for further investigations aimed to find new potential drugs for the photochemotherapy of skin cancer.
Effect of Row Spacing and Seeding Rate on Russian Thistle (Salsola tragus) in Spring Barley and Spring Wheat
Authors: Barroso Judit , Genna Nicholas G.
Published: January 2021

Russian thistle (Salsola tragus L.) is a persistent post-harvest issue in the Pacific Northwest (PNW). Farmers need more integrated management strategies to control it. Russian thistle emergence, mortality, plant biomass, seed production, and crop yield were evaluated in spring wheat and spring barley planted in 18- or 36-cm row spacing and seeded at 73 or 140 kg ha−1 in Pendleton and Moro, Oregon, during 2018 and 2019. Russian thistle emergence was lower and mortality was higher in spring barley than in spring wheat. However, little to no effect of row spacing or seeding rate was observed on Russian thistle emergence or mortality. Russian thistle seed production and plant biomass followed crop productivity; higher crop yield produced higher Russian thistle biomass and seed production and lower crop yield produced lower weed biomass and seed production. Crop yield with Russian thistle pressure was improved in 2018 with 18-cm rows or by seeding at 140 kg ha−1 while no effect was observed in 2019. Increasing seeding rates or planting spring crops in narrow rows may be effective at increasing yield in low rainfall years of the PNW, such as in 2018. No effect may be observed in years with higher rainfall than normal, such as in 2019.
Key Genes in the Melatonin Biosynthesis Pathway with Circadian Rhythm Are Associated with Various Abiotic Stresses
Authors: Ahn Hye-Ryun , Kim Yu-Jin , Lim You-Jin , Duan Shucheng , Eom Seok-Hyun , Jung Ki-Hong
Published: January 2021

Melatonin (N-acetyl-5-methoxytryptamine), a well-known animal hormone, is involved in several biological processes including circadian rhythm and the regulation of abiotic stress. A systematic understanding of the circadian regulation of melatonin biosynthesis-related genes has not been achieved in rice. In this study, key genes for all of the enzymes in the melatonin biosynthetic pathway that showed a peak of expression at night were identified by microarray data analysis and confirmed by qRT–PCR analysis. We further examined the expression patterns of the four genes under drought, salt, and cold stresses. The results showed that abiotic stresses, such as drought, salt, and cold, affected the expression patterns of melatonin biosynthetic genes. In addition, the circadian expression patterns of tryptophan decarboxylase (TDC), tryptamine 5-hydroxylase (T5H), and serotonin N-acetyltransferase (SNAT) genes in wild-type (WT) plants was damaged by the drought treatment under light and dark conditions. Conversely, N-acetylserotonin O-methyltransferase (ASMT) retained the circadian rhythm. The expression of ASMT was down-regulated by the rice gigantea (OsGI) mutation, suggesting the involvement of the melatonin biosynthetic pathway in the OsGI-mediated circadian regulation pathway. Taken together, our results provide clues to explain the relationship between circadian rhythms and abiotic stresses in the process of melatonin biosynthesis in rice.
The Genus Lagochilus (Lamiaceae): A Review of Its Diversity, Ethnobotany, Phytochemistry, and Pharmacology
Authors: Mamadalieva Nilufar Z. , Akramov Davlat Kh. , Wessjohann Ludger A. , Hussain Hidayat , Long Chunlin , Tojibaev Komiljon Sh. , Alshammari Elham , Ashour Mohamed L. , Wink Michael
Published: January 2021

The genus Lagochilus (Lamiaceae) is native to Central, South-Central, and Eastern Asia. It comprises 44 species, which have been commonly used as herbal medicines for the treatments of various ailments for thousands of years, especially in Asian countries. This review aims to summarize the chemical constituents and pharmacological activities of species from the genus Lagochilus to unveil opportunities for future research. In addition, we provide some information about their traditional uses, botany, and diversity. More than 150 secondary metabolites have been reported from Lagochilus, including diterpenes, flavonoids, phenolic compounds, triterpenoids, iridoid glycosides, lignans, steroids, alkaloids, polysaccharides, volatile, non-volatile and aromatic compounds, lipids, carbohydrates, minerals, vitamins, and other secondary metabolites. In vitro and in vivo pharmacological studies on the crude extracts, fractions, and isolated compounds from Lagochilus species showed hemostatic, antibacterial, anti-inflammatory, anti-allergic, cytotoxic, enzyme inhibitory, antispasmodic, hypotensive, sedative, psychoactive, and other activities.
Responses of Rice Growth to Day and Night Temperature and Relative Air Humidity—Leaf Elongation and Assimilation
Authors: Stuerz Sabine , Asch Folkard
Published: January 2021

Predictions of future crop growth and yield under a changing climate require a precise knowledge of plant responses to their environment. Since leaf growth increases the photosynthesizing area of the plant, it occupies a central position during the vegetative phase. Rice is cultivated in diverse ecological zones largely differing in temperature and relative air humidity (RH). To investigate the effects of temperature and RH during day and night on leaf growth, one variety (IR64) was grown in a growth chamber using 9 day/night regimes around the same mean temperature and RH, which were combinations of 3 temperature treatments (30/20 °C, 25/25 °C, 20/30 °C day/night temperature) and 3 RH treatments (40/90%, 65/65%, 90/40% day/night RH). Day/night leaf elongation rates (LER) were measured and compared to leaf gas exchange measurements and leaf area expansion on the plant level. While daytime LER was mainly temperature-dependent, nighttime LER was equally affected by temperature and RH and closely correlated with leaf area expansion at the plant level. We hypothesize that the same parameters increasing LER during the night also enhance leaf area expansion via shifts in partitioning to larger and thinner leaves. Further, base temperatures estimated from LERs varied with RH, emphasizing the need to take RH into consideration when modeling crop growth in response to temperature.
Identifying Shared Risk Genes between Nonalcoholic Fatty Liver Disease and Metabolic Traits by Cross-Trait Association Analysis
Authors: Guo Hongping , Yu Zuguo
Published: January 2021

Nonalcoholic fatty liver disease (NAFLD) generally co-occurs with metabolic disorders, but it is unclear which genes have a pleiotripic effect on NAFLD and metabolic traits. We performed a large-scale cross-trait association analysis to identify the overlapping genes between NAFLD and nine metabolic traits. Among all the metabolic traits, we found that obesity and type II diabetes are associated with NAFLD. Then, a multitrait association analysis among NAFLD, obesity and type II diabetes was conducted to improve the overall statistical power. We identified 792 significant variants by a cross-trait meta-analysis involving 100 pleiotripic genes. Moreover, we detected another two common genes by a genome-wide gene test. The results from the pathway enrichment analysis show that the 102 shared risk genes are enriched in cancer, diabetes, insulin secretion, and other related pathways. This study can help us understand the molecular mechanisms underlying comorbid NAFLD and metabolic disorders.
Influence of Surface Waviness of Journal and Bearing Bush on the Static Characteristics of Hydrodynamic Bearing
Authors: Yang Minghui , Lu Hong , Zhang Xinbao , Duan Meng , Bao Liu , Wang Bowen , Wu Wan
Published: January 2021

An investigation on the surface waviness of both the journal and the bearing bush and their impact on the static characteristics of the hydrodynamic journal bearing is presented in this paper. The finite difference method is introduced to solve a Reynolds equation and obtain the unknown pressure field. The static characteristics, including the load carrying capacity, attitude angle, end leakage flow rate and frictional coefficient are studied under different waviness parameters. The numerically simulated results indicate that the waviness of the bearing bush may deteriorate or enhance the bearing system, depending on the phase angle. The waviness of the journal causes periodic changes in bearing behavior, owing to the alteration in the phase angle. The profile of the journal and bearing surfaces near the attitude angle determines the performance of the bearing system.
Corrosion Study on Wellbore Materials for the CO2 Injection Process
Authors: Hoa Le Quynh , Bäßler Ralph , Bettge Dirk , Buggisch Enrico , Schiller Bernadette Nicole , Beck Matthias
Published: January 2021

For reliability and safety issues of injection wells, corrosion resistance of materials used needs to be determined. Herein, representative low-cost materials, including carbon steel X70/1.8977 and low alloyed steel 1.7225, were embedded in mortar to mimic the realistic casing-mortar interface. Two types of cement were investigated: (1) Dyckerhoff Variodur commercial Portland cement, representing a highly acidic resistant cement and (2) Wollastonite, which can react with CO2 and become stable under a CO2 stream due to the carbonation process. Exposure tests were performed under 10 MPa and at 333 K in artificial aquifer fluid for up to 20 weeks, revealing crevice corrosion and uniform corrosion instead of expected pitting corrosion. To clarify the role of cement, simulated pore water was made by dispersing cement powder in aquifer fluid and used as a solution to expose steels. Surface analysis, accompanied by element mapping on exposed specimens and their cross-sections, was carried out to trace the chloride intrusion and corrosion process that followed.
Optimization of Antimony Removal by Coagulation-Flocculation-Sedimentation Process Using Response Surface Methodology
Authors: Inam Muhammad Ali , Khan Rizwan , Yeom Ick Tae , Buller Abdul Salam , Akram Muhammad , Inam Muhammad Waleed
Published: January 2021

Coprecipitation-adsorption plays a significant role during coagulation-flocculation-sedimentation (C/F/S) of antimony (Sb) in water. This work uses a Box–Behnken statistical experiment design (BBD) and response surface methodology (RSM) to investigate the effects of major operating variables such as initial Sb(III, V) concentration (100–1000 µg/L), ferric chloride (FC) dose (5–50 mg/L), and pH (4–10) on redox Sb species. Experimental data of Sb(III, V) removal were used to determine response function coefficients. The model response value (Sb removal) showed good agreement with the experimental results. FC showed promising coagulation behavior of both Sb species under optimum pH (6.5–7.5) due to its high affinity towards Sb species and low residual Fe concentration. However, a high dose of 50 mg/L of FC is required for the maximum (88–93%) removal of Sb(V), but also for the highest (92–98%) removal of low initial concentrations of Sb(III). Furthermore, BBD and RSM were found to be reliable and feasible for determining the optimum conditions for Sb removal from environmental water samples by a C/F/S process. This work may contribute to a better understanding and prediction of the C/F/S behavior of Sb(III, V) species in aqueous environments, to reduce potential risks to humans.
Impact of Altered Trehalose Metabolism on Physiological Response of Penicillium chrysogenum Chemostat Cultures during Industrially Relevant Rapid Feast/Famine Conditions
Authors: Wang Xinxin , Zhao Jiachen , Xia Jianye , Wang Guan , Chu Ju , Zhuang Yingping
Published: January 2021

Due to insufficient mass transfer and mixing issues, cells in the industrial-scale bioreactor are often forced to experience glucose feast/famine cycles, mostly resulting in reduced commercial metrics (titer, yield and productivity). Trehalose cycling has been confirmed as a double-edged sword in the Penicillium chrysogenum strain, which facilitates the maintenance of a metabolically balanced state, but it consumes extra amounts of the ATP responsible for the repeated breakdown and formation of trehalose molecules in response to extracellular glucose perturbations. This loss of ATP would be in competition with the high ATP-demanding penicillin biosynthesis. In this work, the role of trehalose metabolism was further explored under industrially relevant conditions by cultivating a high-yielding Penicillium chrysogenum strain, and the derived trehalose-null strains in the glucose-limited chemostat system where the glucose feast/famine condition was imposed. This dynamic feast/famine regime with a block-wise feed/no feed regime (36 s on, 324 s off) allows one to generate repetitive cycles of moderate changes in glucose availability. The results obtained using quantitative metabolomics and stoichiometric analysis revealed that the intact trehalose metabolism is vitally important for maintaining penicillin production capacity in the Penicillium chrysogenum strain under both steady state and dynamic conditions. Additionally, cells lacking such a key metabolic regulator would become more sensitive to industrially relevant conditions, and are more able to sustain metabolic rearrangements, which manifests in the shrinkage of the central metabolite pool size and the formation of ATP-consuming futile cycles.
Chlorine-Resistant Hollow Nanosphere-Like VOx/CeO2 Catalysts for Highly Selective and Stable Destruction of 1,2-Dichloroethane: Byproduct Inhibition and Reaction Mechanism
Authors: Huang Yu , Fang Shiyue , Tian Mingjiao , Jiang Zeyu , Wu Yani , He Chi
Published: January 2021

Developing economical and robust catalysts for the highly selective and stable destruction of chlorinated volatile organic compounds (CVOCs) is a great challenge. Here, hollow nanosphere-like VOx/CeO2 catalysts with different V/Ce molar ratios were fabricated and adopted for the destruction of1,2–dichloroethane (1,2–DCE). The V0.05Ce catalyst possessed superior catalytic activity, reaction selectivity, and chlorine resistance owing to a large number of oxygen vacancies, excellent low-temperature redox ability, and chemically adsorbed oxygen (O− and O2−) species mobility. Typical chlorinated byproducts (CHCl3, CCl4, C2HCl3, and C2H3Cl3) derived from the cleavage of C–Cl and C–C bonds of 1,2–DCE were detected, which could be effectively inhibited by the abundant acid sites and the strong interactions of VOx species with CeO2. The presence of water vapor benefited the activation and deep destruction of 1,2–DCE over V0.05Ce owing to the efficient removal of Cl species from the catalyst surface.
Stratification Analysis and Behaviour of a Real Industrial Thermocline Thermal Energy Storage Tank for Cogeneration Purposes
Authors: Fernández Francisco , Díaz José , Folgueras María , Suárez Inés
Published: January 2021

Thermal energy storage systems help to couple thermal energy generation and process demand in cogeneration facilities. One single deposit with two design temperatures and one main temperature step in sensible thermal energy storage define the thermocline systems. Performance of one high size real thermocline thermal energy storage system is analysed. Starting from temperature and mass flow rate data registered by the plant control system, one advanced thermodynamic analysis is performed. The quality of heat storage is analysed in terms of evaluation of the stratification in the thermocline zone. The temperature data registered at 21 positions is extended by displacement analysis generating detailed profiles. Fraction of recoverable heat, thermocline width, stratification indices based on energy and exergy analysis, and mean temperature gradients in the thermocline region are calculated. These parameters are monitored under real operation conditions of the plant. The calculated parameters are studied to check their distribution and correlation. First and Second Law indices show parallel behaviour and two values are found that delimit situations of high and low values of mean temperature gradients. It was observed that buoyancy generates uniform forced movement with the right water temperature entering the diffusers, but good control strategies are essential to avoid mixing. The system demonstrated great stability in this use.
Special Issue on “Recent Advances in Population Balance Modeling”
Authors: Mansouri Seyed Soheil , Briesen Heiko , Gernaey Krist V. , Nopens Ingmar
Published: January 2021

Population Balance Modeling (PBM) is a powerful modeling framework that allows the prediction of the dynamics of distributed properties of a population of individuals at the mesoscale [...]
Biodiesel from Crude Tall Oil and Its NOx and Aldehydes Emissions in a Diesel Engine Fueled by Biodiesel-Diesel Blends with Water Emulsions
Authors: Roy Murari Mohon , Islam Md Shariful , Alam Md Nur
Published: January 2021

Using biodiesel in diesel engines is beneficial for reducing emissions of carbon monoxide (CO), hydrocarbons (HC) and particulate matters (PM). Biodiesel is usually produced from vegetable oils or animal fats. When produced from plant oil or woody plant sources, biodiesel can reduce a significant amount of carbon dioxide on a life cycle basis. The objective of this study is to produce biodiesel from a non-conventional woody plant source that is, crude tall oil, which is a dark brown viscous liquid extracted and processed in wood pulping plants. It contains a high percentage of fatty acids. From raw crude tall oil, tall oil fatty acids were separated and were successfully used for the production of biodiesel in this study. Although biodiesel produces lower CO, HC and PM than petroleum diesel fuel, it produces higher oxides of nitrogen (NOx) emissions in diesel engines. Water emulsifications of diesel-biodiesel blends are investigated in a direct injection (DI) diesel engine in this work to understand their potential for NOx reduction. When using 10% water in the emulsions, NOx was reduced by nearly 15%. In aldehyde emissions, B100 showed 35% lower aldehydes and B100 with 10% water emulsion produced nearly 90% lower aldehydes than diesel fuel—a substantial reduction. Therefore, this study accomplished the desired goal of producing biodiesel from a non-conventional source, which satisfies ASTM biodiesel standard and results in lower NOx and aldehydes emissions with water emulsifications of diesel-biodiesel blends in a diesel engine compared to that of diesel fuel.
In-Situ Heavy Oil Aquathermolysis in the Presence of Nanodispersed Catalysts Based on Transition Metals
Authors: Aliev Firdavs A. , Mukhamatdinov Irek I. , Sitnov Sergey A. , Ziganshina Mayya R. , Onishchenko Yaroslav V. , Sharifullin Andrey V. , Vakhin Alexey V.
Published: January 2021

The aquathermolysis process is widely considered to be one of the most promising approaches of in-situ upgrading of heavy oil. It is well known that introduction of metal ions speeds up the aquathermolysis reactions. There are several types of catalysts such as dispersed (heterogeneous), water-soluble and oil soluble catalysts, among which oil-soluble catalysts are attracting considerable interest in terms of efficiency and industrial scale implementation. However, the rock minerals of reservoir rocks behave like catalysts; their influence is small in contrast to the introduced metal ions. It is believed that catalytic aquathermolysis process initiates with the destruction of C-S bonds, which are very heat-sensitive and behave like a trigger for the following reactions such as ring opening, hydrogenation, reforming, water–gas shift and desulfurization reactions. Hence, the asphaltenes are hydrocracked and the viscosity of heavy oil is reduced significantly. Application of different hydrogen donors in combination with catalysts (catalytic complexes) provides a synergetic effect on viscosity reduction. The use of catalytic complexes in pilot and field tests showed the heavy oil viscosity reduction, increase in the content of light hydrocarbons and decrease in heavy fractions, as well as sulfur content. Hence, the catalytic aquathermolysis process as a distinct process can be applied as a successful method to enhance oil recovery. The objective of this study is to review all previously published lab scale and pilot experimental data, various reaction schemes and field observations on the in-situ catalytic aquathermolysis process.
Controlling Nanoparticle Formulation: A Low-Budget Prototype for the Automation of a Microfluidic Platform
Authors: Loy Dominik M. , Krzysztoń Rafał , Lächelt Ulrich , Rädler Joachim O. , Wagner Ernst
Published: January 2021

Active pharmaceutical ingredients (API) with suboptimal pharmacokinetic properties may require formulation into nanoparticles. In addition to the quality of the excipients, production parameters are crucial for producing nanoparticles which reliably deliver APIs to their target. Microfluidic platforms promise increased control over the formulation process due to the decreased degrees of freedom at the micro- and nanoscale. Publications about these platforms usually provide only limited information about the soft- and hardware required to integrate the microfluidic chip seamlessly into an experimental set-up. We describe a modular, low-budget prototype for microfluidic mixing in detail. The prototype consists of four modules. The control module is a raspberry pi executing customizable python scripts to control the syringe pumps and the fraction collector. The feeding module consists of up to three commercially available, programable syringe pumps. The formulation module can be any macro- or microfluidic chip connectable to syringe pumps. The collection module is a custom-built fraction collector. We describe each feature of the working prototype and demonstrate its power with polyplexes formulated from siRNA and two different oligomers that are fed to the chip at two different stages during the assembly of the nanoparticles.
Effect of Grape Seed Flour on the Antioxidant Profile, Textural and Sensory Properties of Waffles
Authors: Antonic Bojan , Dordevic Dani , Jancikova Simona , Holeckova Denisa , Tremlova Bohuslava , Kulawik Piotr
Published: January 2021

The study aimed to enrich waffle products with grape seed flour, but without affecting their sensory properties. The grape seed flour was obtained from grape pomace, and as an ingredient rich in bioactive compounds such as fiber, polyphenols and minerals, it was used to fortify waffles in concentrations of 1, 3, 5 and 10%. The results for the total polyphenolic content, ferric ion reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) inhibition, showed the highest effect with 10% grape seed flour fortification, including 1.25 mg of gallic acid equivalent/g, 5.62 µmol Trolox/g and 26.65% DPPH inhibition, respectively. Texture analysis revealed an increase in hardness of the waffles along with the increase of grape seed flour percentage, while there was no evident change in moisture in any of the tested samples. Sensory evaluation showed no significant differences regarding overall impression or the estimated price value for all samples, even though evident changes were noted by the panelists in their color, consistency and sweetness. It may be concluded that fortification was successfully carried out, and that there is a great potential for utilizing grape seed flour as a by-product in the enrichment of products such as waffles.
After Hajj: Muslim Pilgrims Refashioning Themselves
Authors: Al-Ajarma Kholoud
Published: January 2021

The Muslim pilgrimage to Mecca (Hajj) is one of the five pillars of Islam and a duty which Muslims must perform—once in a lifetime—if they are physically and financially able to do so. In Morocco, from where thousands of pilgrims travel to Mecca every year, the Hajj often represents the culmination of years of preparation and planning, both spiritual and logistical. Pilgrims often describe their journey to Mecca as a transformative experience. Upon successfully completing the pilgrimage and returning home, pilgrims must negotiate their new status—and the expectations that come with it—within the mundane and complex reality of everyday life. There are many ambivalences and tensions to be dealt with, including managing the community expectations of piety and moral behavior. On a personal level, pilgrims struggle between staying on the right path, faithful to their pilgrimage experience, and straying from that path as a result of human imperfection and the inability to sustain the ideals inspired by pilgrimage. By ethnographically studying the everyday lives of Moroccans after their return from Mecca, this article seeks to answer the questions: how do pilgrims encounter a variety of competing expectations and demands following their pilgrimage and how are their efforts received by members of their community? How do they shape their social and religious behavior as returned pilgrims? How do they deal with the tensions between the ideals of Hajj and the realities of daily life? In short, this article scrutinizes the religious, social and personal ramifications for pilgrims after the completion of Hajj and return to their community. My research illustrates that pilgrimage contributes to a process of self-formation among pilgrims, with religious and non-religious dimensions, which continues long after Hajj is over and which operates within, and interacts with, specific social contexts.
The Romanian Orthodox Church, the European Union and the Contention on Human Rights
Authors: Guglielmi Marco
Published: January 2021

Since the 1990s, there has been conflictual interactions between Orthodox Christian churches and human rights in South Eastern Europe, especially during the process of European integration. In this work, I shall concentrate on the case of the Romanian Orthodox Church and explore its current position towards human rights that has developed within the context of EU membership. Focusing on the influence that European integration has had on the Romanian Orthodox Church, I hypothesise a re-orientation of the latter from a position of closure and a general rejection of human rights in the direction of their partial acceptance, with this being related to its attempt to develop a European identity.
Influence of the COVID-19 Crisis on Steel Production in Poland Compared to the Financial Crisis of 2009 and to Boom Periods in the Market
Authors: Gajdzik Bożena , Wolniak Radoslaw
Published: January 2021

This paper presents an analysis of the volume of steel production in Poland during the COVID-19 crisis in the first half of 2020 in comparison to the volume of steel production during the financial crisis initiated in the US during the period 2007–2008, whose effects, in the form of a large decrease in steel production, were seen in 2009 in Poland. A comparison is also made to periods of prosperity in 2004, 2007, and 2017 (when there was a good economic situation in the steel market in Poland). The selection of the time period—the first half of 2020—was based on the emergence of a new situation in the economy, which was lockdown. The aim of the analysis is to determine the impact of the COVID-19 situation on the steel market (volume of steel production) in Poland. The analysis performed could help entrepreneurs manage their companies during the COVID-19 crisis. This paper belongs to the category of research work. The statistical analysis was realized regarding steel production in Poland. Three periods were analyzed: The first half of 2020—the period termed the COVID-19 crisis; the year 2019—the year of a large decrease in steel production in Poland caused by the world financial crisis; and periods of prosperity in the steel market—the years 2004, 2007, and 2017 (periods before crises). The analysis shows that, in order to assess the impact of the COVID-19 crisis on the functioning of enterprises or industries, it is necessary to analyze the situation and compare it with other situations in the past. Moreover, crisis management in the COVID-19 situation must be highly rationalized and real, and the various industrial sectors and companies forming them should adapt this process to their own situation. Results: On the basis of the statistical data, it was found that, in the short term (months), the production of steel during the COVID-19 crisis was a little higher than in the financial crisis of 2009 (excluding steel production in June 2020), and lower than during the boom in the steel market (the comparison to the periods when there was a boom in the Polish steel market was made to show the dynamics of decline).
An Expectation-Maximization Algorithm for the Exponential-Generalized Inverse Gaussian Regression Model with Varying Dispersion and Shape for Modelling the Aggregate Claim Amount
Authors: Tzougas George , Jeong Himchan
Published: January 2021

This article presents the Exponential–Generalized Inverse Gaussian regression model with varying dispersion and shape. The EGIG is a general distribution family which, under the adopted modelling framework, can provide the appropriate level of flexibility to fit moderate costs with high frequencies and heavy-tailed claim sizes, as they both represent significant proportions of the total loss in non-life insurance. The model’s implementation is illustrated by a real data application which involves fitting claim size data from a European motor insurer. The maximum likelihood estimation of the model parameters is achieved through a novel Expectation Maximization (EM)-type algorithm that is computationally tractable and is demonstrated to perform satisfactorily.
Adaptive Human-Robot Interactions for Multiple Unmanned Aerial Vehicles
Authors: Lim Yixiang , Pongsakornsathien Nichakorn , Gardi Alessandro , Sabatini Roberto , Kistan Trevor , Ezer Neta , Bursch Daniel J.
Published: January 2021

Advances in unmanned aircraft systems (UAS) have paved the way for progressively higher levels of intelligence and autonomy, supporting new modes of operation, such as the one-to-many (OTM) concept, where a single human operator is responsible for monitoring and coordinating the tasks of multiple unmanned aerial vehicles (UAVs). This paper presents the development and evaluation of cognitive human-machine interfaces and interactions (CHMI2) supporting adaptive automation in OTM applications. A CHMI2 system comprises a network of neurophysiological sensors and machine-learning based models for inferring user cognitive states, as well as the adaptation engine containing a set of transition logics for control/display functions and discrete autonomy levels. Models of the user’s cognitive states are trained on past performance and neurophysiological data during an offline calibration phase, and subsequently used in the online adaptation phase for real-time inference of these cognitive states. To investigate adaptive automation in OTM applications, a scenario involving bushfire detection was developed where a single human operator is responsible for tasking multiple UAV platforms to search for and localize bushfires over a wide area. We present the architecture and design of the UAS simulation environment that was developed, together with various human-machine interface (HMI) formats and functions, to evaluate the CHMI2 system’s feasibility through human-in-the-loop (HITL) experiments. The CHMI2 module was subsequently integrated into the simulation environment, providing the sensing, inference, and adaptation capabilities needed to realise adaptive automation. HITL experiments were performed to verify the CHMI2 module’s functionalities in the offline calibration and online adaptation phases. In particular, results from the online adaptation phase showed that the system was able to support real-time inference and human-machine interface and interaction (HMI2) adaptation. However, the accuracy of the inferred workload was variable across the different participants (with a root mean squared error (RMSE) ranging from 0.2 to 0.6), partly due to the reduced number of neurophysiological features available as real-time inputs and also due to limited training stages in the offline calibration phase. To improve the performance of the system, future work will investigate the use of alternative machine learning techniques, additional neurophysiological input features, and a more extensive training stage.
Exploring Robot Connectivity and Collaborative Sensing in a High-School Enrichment Program
Authors: Verner Igor M. , Cuperman Dan , Reitman Michael
Published: January 2021

Education is facing challenges to keep pace with the widespread introduction of robots and digital technologies in industry and everyday life. These challenges necessitate new approaches to impart students at all levels of education with the knowledge of smart connected robot systems. This paper presents the high-school enrichment program Intelligent Robotics and Smart Transportation, which implements an approach to teaching the concepts and skills of robot connectivity, collaborative sensing, and artificial intelligence, through practice with multi-robot systems. The students used a simple control language to program Bioloid wheeled robots and utilized Phyton and Robot Operating System (ROS) to program Tello drones and TurtleBots in a Linux environment. In their projects, the students implemented multi-robot tasks in which the robots exchanged sensory data via the internet. Our educational study evaluated the contribution of the program to students’ learning of connectivity and collaborative sensing of robot systems and their interest in modern robotics. The students’ responses indicated that the program had a high positive contribution to their knowledge and skills and fostered their interest in the learned subjects. The study revealed the value of learning of internet of things and collaborative sensing for enhancing this contribution.