bluuebunny/crossref_metadata_2025 huggingface dataset:
STL-DCSInformer-ETS: A Hybrid Model for Medium- and Long-Term Sales Forecasting of Fast-Moving Consumer Goods
Authors: Ma Yecheng , He Lili , Zheng Junhong
Published: February 2025

Accurately forecasting sales for fast-moving consumer goods (FMCG) remains a significant challenge due to the volatile and multi-faceted nature of sales data. Existing methods often struggle to capture intricate patterns driven by seasonal trends, external factors, and consumer behavior, hindering effective inventory management and strategic decision-making. To overcome these challenges, we propose STL-DCSInformer-ETS, a hybrid model that integrates three complementary components: STL decomposition, an enhanced DCSInformer model, and the ETS model. The model uses monthly sales data from a FMCG company, with key features including sales volume, product prices, promotional activities, and regulatory factors such as holidays, geographical information, consumer behavior, product factors, etc. STL decomposition partitions time-series data into trend, seasonal, and residual components, reducing data complexity and enabling more targeted forecasting. The enhanced DCSInformer employs dilated causal convolution and a multi-scale feature extraction mechanism to capture long-term dependencies and short-term variations effectively. Meanwhile, the ETS model specializes in modeling seasonal patterns, further refining forecasting precision. To further improve predictive performance, the Random Forest-based Recursive Feature Elimination (RF-RFE) method is applied to optimize feature selection. RF-RFE identifies key predictive factors from multiple dimensions, such as time, geography, and economy, which significantly influence forecasting accuracy. Through numerical experiments, the method demonstrates excellent performance by achieving a 35.9% reduction in Mean Squared Error and a 21.4% decrease in Mean Absolute Percentage Error, significantly outperforming traditional methods. Furthermore, the model effectively captures both medium- and long-term sales trends while addressing short-term fluctuations, leading to more accurate forecasting and improved decision-making for fast-moving consumer goods. This research provides new theoretical insights into hybrid forecasting models and practical solutions for optimizing inventory management and strategic planning in the FMCG industry.
Dual-Modality Transformer with Time Series Imaging for Robust Epileptic Seizure Prediction
Authors: Qin Jiahao , Liu Zijia , Zhuang Jihong , Liu Feng
Published: February 2025

Automated EEG classification algorithms for seizures can facilitate the clinical diagnosis of epilepsy, enabling more expedient and precise classification. However, existing EEG signal preprocessing methods oriented towards artifact removal and signal enhancement have demonstrated suboptimal accuracy and robustness. In response to this challenge, we propose an Adaptive Dual-Modality Learning Model (ADML) for epileptic seizure prediction by combining time series imaging with Transformer-based architecture. Our approach effectively captures both temporal dependencies and spatial relationships in EEG signals through a specialized attention mechanism. Evaluated on the CHB-MIT and Bonn datasets, our method achieves 98.7% and 99.2% accuracy, respectively, significantly outperforming existing approaches. The model demonstrates strong generalization capability across datasets while maintaining computational efficiency. Cross-dataset validation confirms the robustness of our approach, with consistent performance above 96% accuracy. These results suggest that our dual-modality approach provides a reliable and practical solution for clinical epileptic seizure prediction.
Use of Wickerhamomyces anomalus Strains from Biologically Aged Wines to Improve the Sensorial Profile of Young White Wines
Authors: Carbonero-Pacheco Juan , García-Jiménez Álvaro , García-García Juan Carlos , Santos-Dueñas Inés M. , García-Martínez Teresa , Moreno Juan , Moreno-García Jaime , Mauricio Juan Carlos
Published: February 2025

Non-Saccharomyces yeasts play a significant role in winemaking, offering unique benefits and contributing to wine complexity and varied and desirable aromatic profiles. This work focuses on the sensory improvement of Pedro Ximénez white wines using selected strains of Wickerhamomyces anomalus isolated from biologically aged wines. Chemical and microbiological analyses confirmed the implantation of W. anomalus; these yeast strains appear to displace indigenous non-Saccharomyces species in the must and produce large amounts of ethyl acetate and lower ethanol content. Wines made with W. anomalus strains were judged negatively by the tasting panel due to a nail polish/varnish odor and a strong, bitter taste; however, when these wines were blended with wine normally produced by spontaneous fermentation, the judges rated them positively, highlighting fruity aromas not detected under other conditions. These results conclude that W. anomalus strains isolated from biologically aged wines could be useful for modulating the sensory profile of white wines. Moreover, their use in combination with other yeasts or in immobilized form could improve the results obtained and avoid the blending process. The high yield of ethyl acetate produced by these strains could be of interest as an alternative to current methods of producing this compound, including the use of these wines for the production of quality vinegar.
Exploring the Bioactive Potential of Mushroom Aqueous Extracts: Antimicrobial, Antioxidant, and Prebiotic Properties
Authors: Afonso Tiago Barros , Marçal Sara , Vale Pedro , Sousa Ana Sofia , Nunes João , Pintado Manuela
Published: February 2025

Mushrooms provide essential nutrients and bioactive compounds, namely glucans, protein, and phenolic compounds. In this study, two aqueous extractions were performed (M1 and M2) using Pleurotus ostreatus, Pleurotus eryngii, and Agrocybe cylindracea. In M1, a hot extraction (extract M1) (90 °C, 700 rpm, 1 h) was performed. In M2, a room-temperature extraction (extract M2A) followed by a hot extraction (extract M2B) (90 °C, 700 rpm, 1 h) of the extract M2A residue was performed. The M2B extracts showed the lowest extraction yields (12.58–21.78%), while the other yields ranged between 30.91% and 46.03%. All extracts had high protein (12.09–32.97 g/100 g of dry extract), glucan (12.69–48.57 g/100 g of dry extract), and phenolic contents (7.90–16.65 mg GAEs/g of dry extract) and high antioxidant (ABTS and ORAC assays), antimicrobial, antibiofilm, and prebiotic activities. So, they have potential to be used as functional ingredients or natural preservatives. Extracts from A. cylindracea stood out since they had higher protein content, antioxidant activity, and prebiotic activity (extract M1) and inhibited a higher number of foodborne bacteria (only extract M2A). However, unlike extracts from P. ostreatus and P. eryngii, at 40 mg/mL, they had cytotoxic effects.
Glycine Betaine Levels and BADH Activity of Juvenile Shrimp Litopenaeus vannamei in Response to Vibrio Bacterial Infection and Sudden Hyperosmotic Stress
Authors: Gomez-Jimenez Silvia , Valenzuela-Soto Elisa M. , Zamorano-Apodaca Julio C. , Gamez-Alejo Luis A. , Muñoz-Bacasehua Cesar
Published: February 2025

High evaporation rates due to solar intensity and low precipitation could represent a challenging culture environment in northwestern Mexico, generating osmotic stress in shrimp due to high salinity. Bacterial infections by pathogenic Vibrio strains are highly virulent in shrimp culture. This study evaluated betaine aldehyde dehydrogenase (BADH) activity and glycine betaine (GB) levels in Litopenaeus vannamei under high salinity levels plus experimental infection with virulent Vibrio parahaemolyticus. At 35 ppt (control group) and 40 ppt after infection, GB levels increased two-fold in the gills except at 45 ppt and were significantly higher at 50 ppt. The highest GB levels were in the hepatopancreas of the uninfected group at 45 ppt. In the gills, BADH activity decreased after 2 h of exposure at 40 and 45 ppt; at 50 ppt, there was a significant increase in the uninfected groups. However, upon infection, activity increased at all salinities except 50 ppt. In the hepatopancreas of the uninfected groups, the highest activity was at 40 ppt and this was lowest at 50 ppt after 8 h. In the muscles, BADH was detectable at all salinities; infection caused an increase in its activity at 45 and 50 ppt. Despite sudden exposure to high salinity plus experimental infection, our results show that Litopenaeus vannamei does not inhibit BADH activity, allowing GB synthesis, which may play a role in shrimp survival under these conditions.
Analysis of an Abstract Delayed Fractional Integro-Differential System via the α-Resolvent Operator
Authors: Khan Ishfaq , Zada Akbar , Popa Ioan-Lucian , Kallekh Afef
Published: February 2025

This paper explores the mild solutions of partial impulsive fractional integro-differential systems of order 1<α<2 in a Banach space. We derive the solution of the system under the assumption that the homogeneous part of the system admits an α-resolvent operator. Krasnoselskii’s fixed point theorem is used for the existence of solution, while uniqueness is ensured using Banach’s fixed point theorem. The stability of the system is analyzed through the framework of Hyers–Ulam stability using Lipschitz conditions. Finally, examples are presented to illustrate the applicability of the theoretical results.
Biofilm Inhibition: The Role of Fixed Oil from Caryocar coriaceum in Fighting Resistant Bacterial Communities
Authors: Almeida-Bezerra José Weverton , da Costa Silva José Thyálisson , Menezes Saulo Almeida , Alencar Gabriel Gonçalves , Ribeiro Daiany Alves , Melo Bárbara Fernandes , da Silva-Junior João Pereira , de Fátima Silva de Sousa Francisca , de Sousa Severino Denicio Gonçalves , dos Santos Marcos Aurélio Figueirêdo , de Souza Dieferson Leandro , Vitor Luiz Neldecílio Alves , Filho Ademar Maia , Felício Murilo Felipe , Andrade-Pinheiro Jacqueline Cosmo , da Silva Viviane Bezerra , Bezerra José Jailson Lima , Morais-Braga Maria Flaviana Bezerra , Coutinho Henrique Douglas Melo
Published: February 2025

Biofilms, formed by microbial communities that increase resistance to antibiotics, are responsible for chronic infections, making their combat a therapeutic priority. Taking this into account, the fruit Caryocar coriaceum stands out for its potential in the treatment of infectious diseases. The different parts of this plant can be used, and the fixed oil extracted from its fruit, rich in fatty acids, is indicated as responsible for its biological activities. Thus, the objective of this study was to evaluate the chemical composition of the fixed oil extracted from the fruits of C. coriaceum (FOCC), in addition to analyzing its action in the inhibition and pre-formed biofilm disruption of bacteria. The fixed oil was extracted from the internal mesocarp through exhaustive extraction with n-hexane, resulting in a yield of 38.29%. For antibiofilm evaluation, multidrug-resistant bacterial strains were exposed to the oil, and the antibiofilm activity was verified through biofilm formation and pre-formed biofilm disruption assays. The chemical analysis of the fixed oil of C. coriaceum (FOCC) identified eight fatty acids, representing 98.2% of the total composition, with a predominance of oleic acid (60.1%) and palmitic acid (33.5%). FOCC demonstrated approximately 70% inhibition of Streptococcus mutans biofilm formation at a concentration of 10 mg/mL and approximately 60% inhibition against Staphylococcus aureus and Pseudomonas aeruginosa. In pre-formed biofilm disruption, FOCC showed low efficacy against S. mutans and P. aeruginosa but showed greater activity against Enterococcus faecalis and S. aureus. These results indicate that FOCC has the potential to prevent biofilms, but its pre-formed biofilm disruption capacity is still limited.
Online Cell-by-Cell Calibration Method to Enhance the Kalman-Filter-Based State-of-Charge Estimation
Authors: Pham Ngoc-Thao , La Phuong-Ha , Kwon Sungoh , Choi Sung-Jin
Published: February 2025

Kalman filter (KF) is an effective way to estimate the state-of-charge (SOC), but its performance is heavily dependent on the state-space model parameters. One of the factors that causes the model parameters to change is battery aging, which is individually and non-uniformly experienced by the cells inside the battery pack. To mitigate this issue, this paper proposes an online calibration method considering the impact of cell aging and cell inconsistency. In this method, the state-of-health (SOH) levels of the individual cells are estimated using the deep learning method, and the historical parameter loop-up table is constructed to update the state-space model. The proposed calibration framework provides enhanced accuracy for cell-by-cell SOC estimation by lightweight computing devices. The SOC estimation errors of the calibrated EKF reduce to 1.81% compared to 12.1% of the uncalibrated algorithms.
Characterization of Normal and Degenerative Discovertebral Complexes Using Qualitative and Quantitative Magnetic Resonance Imaging at 4.7T: Longitudinal Evaluation of Immature and Mature Rats
Authors: Dallaudière Benjamin , Ribot Emeline J. , Trotier Aurélien J. , Dallet Laurence , Thibaudeau Olivier , Miraux Sylvain , Hauger Olivier
Published: February 2025

Purpose: We assessed the feasibility of qualitative, semiquantitative, and multiparametric quantitative magnetic resonance imaging (MRI) using a three-dimensional (3D) ultrashort echo time (3D-UTE) sequence together with 2D-T2 and 3D-T1 mapping sequences to evaluate normal and pathological discovertebral complexes (DVCs). We assessed the disc (nucleus pulposus [NP] and annulus fibrosus [AF]), vertebral endplate (cartilage endplate [CEP] and growth plate [GP]), and subchondral bone (SB) using a rat model of degenerative disc disease (DDD). We also assessed whether this complete MRI cartography can improve the monitoring of DDD. Methods: DDD was induced by percutaneous disc trituration and collagenase injection of the tail. Then, the animals were imaged at 4.7T. The adjacent disc served as the control. The MRI protocol was performed at baseline and each week (W) postoperatively for 2 weeks. Visual analysis and signal intensity measurements from the 3D-UTE images, as well as T2 and T1 measurements, were carried out in all DVC portions. Histological analysis with hematoxylin–eosin and Masson trichrome staining was performed following euthanization of the rats at 2 weeks and the results were compared to the MRI findings. Results: Complete qualitative identification of the normal zonal anatomy of the DVC, including the AF, CEP, and GP, was achieved using the 3D-UTE sequence. Quantitative measurements of the signal-to-noise ratio in the AF and NP enabled healthy DVCs to be distinguished from surgery-induced DDD, based on an increase in these values post-surgery. The 2D-T2 mapping results showed a significant increase in the T2 values of the AF and a decrease in the values of the NP between the baseline and W1 and W2 postoperatively (p < 0.001). In the 3D-T1 mapping, there was a significant decrease in the T1 values of the AF and NP between baseline and W1 and W2 postoperatively in immature rats (p < 0.01). This variation in T1 and T2 over time was consistent with the results of the 3D-UTE sequence. Conclusions: Use of the 3D-UTE sequence enabled a complete, robust, and reproducible visualization of DVC anatomy in both immature and mature rats under both normal and pathological conditions. The findings were supported quantitatively by the T2 and T1 mapping sequences and histologically. This sequence is therefore of prime interest in spinal imaging and should be regularly be performed.
Sex-Specific Analysis of Carotid Artery Through Bilateral 3D Modeling via MRI and DICOM Processing
Authors: Martinez Pedro , Torres Jose Roberto , Conde Daniel , Gomez Manuel , Gurovich Alvaro N.
Published: February 2025

The present study explores the anatomical differences between sexes of the carotid artery using non-invasive magnetic resonance imaging (MRI) and a DICOM processing protocol. Bilateral three-dimensional models of the carotid artery were constructed for 20 healthy young adults, 10 males and 10 females, in order to evaluate key anatomical landmarks; these include the bifurcation diameter and angle, as well as the internal and external carotid arteries (ICA and ECA) for both sides (left and right). The results show that males exhibit larger bifurcation and ECA diameters, which could indicate reduced endothelial shear stress (ESS). However, as there is no previously observed sex difference in ESS between sexes, compensatory factors might be in play, such as blood pressure. This underscores the interaction between vascular geometry and stroke risk disparities; future research is encouraged to analyze diverse demographics and employ flow modeling techniques to further asses the connection between anatomical differences within a given population and vascular outcomes.
Machine Learning- and Deep Learning-Based Myoelectric Control System for Upper Limb Rehabilitation Utilizing EEG and EMG Signals: A Systematic Review
Authors: Zaim Tala , Abdel-Hadi Sara , Mahmoud Rana , Khandakar Amith , Rakhtala Seyed Mehdi , Chowdhury Muhammad E. H.
Published: February 2025

Upper limb disabilities, often caused by conditions such as stroke or neurological disorders, severely limit an individual’s ability to perform essential daily tasks, leading to a significant reduction in quality of life. The development of effective rehabilitation technologies is crucial to restoring motor function and improving patient outcomes. This systematic review examines the application of machine learning and deep learning techniques in myoelectric-controlled systems for upper limb rehabilitation, focusing on the use of electroencephalography and electromyography signals. By integrating non-invasive signal acquisition methods with advanced computational models, the review highlights how these technologies can enhance the accuracy and efficiency of rehabilitation devices. A comprehensive search of literature published between January 2015 and July 2024 led to the selection of fourteen studies that met the inclusion criteria. These studies showcase various approaches in decoding motor intentions and controlling assistive devices, with models such as Long Short-Term Memory Networks, Support Vector Machines, and Convolutional Neural Networks showing notable improvements in control precision. However, challenges remain in terms of model robustness, computational complexity, and real-time applicability. This systematic review aims to provide researchers with a deeper understanding of the current advancements and challenges in this field, guiding future research efforts to overcome these barriers and facilitate the transition of these technologies from experimental settings to practical, real-world applications.
Comparison of Tissue-Engineered Dermis with Micronized Adipose Tissue and Artificial Dermis for Facial Reconstruction Following Skin Cancer Resection
Authors: Lee Kyu-Il , Song Won-Seok , Han Seung-Kyu , Moon Kyung-Chul , Jeong Seong-Ho , Dhong Eun-Sang
Published: February 2025

Our group has previously demonstrated that tissue-engineered dermis containing cultured fibroblasts or adipose-derived stromal vascular fraction cells is superior to artificial dermis in terms of scar quality for covering facial defects. However, using these cells for clinical applications requires Food and Drug Administration approval and involves complex procedures for cell culture or isolation. This retrospective study aimed to compare effects of tissue-engineered dermis containing micronized adipose tissue (MAT) and artificial dermis for facial reconstruction. Tissue-engineered dermis consisting of MAT seeded on artificial dermis was applied in 30 cases, while artificial dermis without MAT was grafted in 35 cases. Healing time and severities of scar contraction, color mismatch, and landmark distortion at one year after healing were evaluated. Wounds in the tissue-engineered dermis group re-epithelialized in 30.0 ± 4.3 days compared to 34.3 ± 5.4 days in the artificial dermis group (p < 0.05). The average dE2000 score in color mismatch analysis was 4.9 ± 1.7 in the tissue-engineered dermis group and 5.1 ± 1.7 in the artificial dermis group (p = 0.57). The extent of scar contraction was 16.2 ± 12.3% in the tissue-engineered dermis group and 23.2 ± 12.8% in the artificial dermis group (p < 0.05). The average severity grade of landmark distortion was 0.20 ± 0.50 in the tissue-engineered dermis group and 0.50 ± 0.71 in the artificial dermis group (p < 0.05). These findings indicate that tissue-engineered dermis grafts containing MAT are superior to artificial dermis grafts for facial reconstruction in terms of healing time, scar contraction, and landmark distortion severity. However, there was no significant difference in color mismatch between the two groups.
Symbionts of Red King Crab from the Sea of Okhotsk: A Review of Russian Studies
Authors: Dvoretsky Alexander G. , Dvoretsky Vladimir G.
Published: February 2025

The red king crab, Paralithodes camtscaticus, is a commercially significant crustacean that supports lucrative fisheries in Russia, the USA, and Norway. The western Kamchatka shelf, located in the Sea of Okhotsk, is home to one of the most important populations of the red king crab. In this study, we have conducted a review of the symbionts associated with P. camtscaticus in the waters off the Kamchatka Peninsula. A total of 42 symbiotic species belonging to 14 different phyla were identified in association with the red king crab. Out of these, 14 species were found to be parasitic to the red king crab, while the remaining 28 were either commensal or epibiont in nature. The taxa with the highest number of associated species included ciliates (11), crustaceans (8), and acanthocephalans (4). Our study found that red king crabs suffering from shell disease exhibited a more diverse symbiotic fauna and higher infestation indices as compared to healthy crabs, which were found to be free from parasites. Dangerous symbionts, such as dinoflagellates Hematodinium sp. and rhizocephalan barnacles Briarosaccus callosus, had low incidence rates, indicating that the red king crab population in the Sea of Okhotsk is in good condition with respect to population abundance, health, and recruitment and is not being adversely impacted by symbiotic organisms.
A Modular Customizable Ligand-Conjugate (LC) System Targeting Ghrelin O-Acyltransferase
Authors: Ford Amber L. , Taft Caine W. , Sprague-Getsy Andrea M. , Carlson Gracie C. , Mate Nilamber A. , Sieburg Michelle A. , Chisholm John D. , Hougland James L.
Published: February 2025

Ghrelin is a 28 amino acid peptide hormone that impacts a wide range of biological processes, including appetite regulation, glucose metabolism, growth hormone regulation, and cognitive function. To bind and activate its cognate receptor, ghrelin must be acylated on a serine residue in a post-translational modification performed by ghrelin O-acyltransferase (GOAT). GOAT is a membrane-bound O-acyltransferase (MBOAT) responsible for the catalysis of the addition of an octanoyl fatty acid to the third serine of desacyl ghrelin. Beyond its canonical role for ghrelin maturation in endocrine cells within the stomach, GOAT was recently reported to be overexpressed in prostate cancer (PCa) cells and detected at increased levels in the serum and urine of PCa patients. This suggests GOAT can serve as a potential route for the detection and therapeutic targeting of PCa and other diseases that exhibit GOAT overexpression. Building upon a ghrelin mimetic peptide with nanomolar affinity for GOAT, we developed an antibody-conjugate-inspired system for customizable ligand-conjugate (LC) synthesis allowing for the attachment of a wide range of cargoes. The developed synthetic scheme allows for the easy synthesis of the desired LCs and demonstrates that our ligand system tolerates an extensive palette of cargoes while maintaining nanomolar affinity against GOAT.
Identification of Common Angiogenesis Marker Genes in Chronic Lung Diseases and Their Relationship with Immune Infiltration Based on Bioinformatics Approaches
Authors: Liu Lu , Wang Man , Yu Shihuan
Published: February 2025

Objective: This study aims to explore the role of angiogenesis-related genes in chronic lung diseases (ILD and COPD) using bioinformatics methods, with the goal of identifying novel therapeutic targets to slow disease progression and prevent its deterioration into fibrosis or pulmonary artery hypertension. Methods: The research methods encompassed differential analysis, WGCNA (Weighted Gene Co-expression Network Analysis), and multiple machine learning approaches to screen for key genes. Gene Set Enrichment Analysis (GSEA), Gene Ontology (GO), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were utilized to assess related biological functions and pathways. Additionally, immune cell infiltration was analyzed to evaluate the immune status of the disease and the correlation between genes and immunity. Results: COPD and ILD are closely associated with pathways related to angiogenesis, immune responses, and others, with differential genes in both groups linked to inflammation-related signaling pathways. The study established a chronic lung disease-related gene set comprising 171 genes and further screened out 21 genes related to angiogenesis. Ultimately, four key genes—COL10A1, EDN1, MMP1, and RRAS—were identified through machine learning methods. These four genes are closely related to angiogenesis and immune processes, and clustering analysis based on them can reflect different disease states and variations in immune cell infiltration. Conclusions: COL10A1, EDN1, MMP1, and RRAS represent potential therapeutic targets for slowing the progression of chronic lung diseases and preventing their deterioration. Furthermore, monocytes exhibited consistent infiltration patterns across disease and control groups, as well as among different subgroups, suggesting their potential significant role in the development of chronic lung diseases.
Investigation of UCHL3 and HNMT Gene Polymorphisms in Greek Patients with Type 2 Diabetes Mellitus and Diabetic Retinopathy
Authors: Flindris Konstantinos , Lagkada Vivian , Christodoulou Aikaterini , Gazouli Maria , Moschos Marilita , Markozannes Georgios , Kitsos George
Published: February 2025

Background and Objectives: Recent studies have shed light on the association between genetic factors and diabetic retinopathy (DR) onset and progression. The purpose of our study was to investigate the association between rs4885322 single-nucleotide polymorphism (SNP) of the UCHL3 gene and rs11558538 SNP of the HNMT gene with the risk of DR in Greek patients with type 2 diabetes mellitus (T2DM). Materials and Methods: In our case–control study, we included 85 T2DM patients with DR and 71 T2DM patients without DR (NDR), matched by ethnicity and gender. Demographic and clinical data of all patients were collected, and then patients went through a complete ophthalmological examination and were genotyped for rs4885322 SNP of UCHL3 gene and for the rs11558538 SNP of HNMT gene. Statistical analysis was implemented by STATA v.16.1. Results: No significant differences in demographic and clinical data were observed between the DR and the NDR group (p-value ≥ 0.05), except for the lower mean of age, longer duration of DM, more frequent use of insulin therapy, and higher levels of hemoglobin A1c (HbA1c) in the DR group. The allelic effect of rs488532 increases the risk of DR by 2.04 times, and in the dominant genetic model, the risk of DR is elevated by 123%, while both associations are statistically significant (p-value < 0.05). Moreover, the allelic effect of rs11558538 is associated with a 3.27 times increased DR risk and, in the dominant genetic model, reveals an augmented risk of DR by 231%, while both associations are also statistically significant (p-value < 0.05). Conclusions: The rs4885322 SNP of the UCHL3 gene and the rs11558538 SNP of the HNMT gene are associated with DR risk in Greek patients with T2DM. However, further studies with larger samples and different ethnicities should be implemented to clarify the exact association of these SNPs and DR onset.
Tetrahydrobiopterin and Autism Spectrum Disorder: A Systematic Review of a Promising Therapeutic Pathway
Authors: Filho Clóvis Colpani , Melfior Lucas , Ramos Sthephanie Luiz , Pizi Mateus Santos Oliveira , Taruhn Lilian Freitas , Muller Margrit Ellis , Nunes Thiago Kucera , Schmitt Luísa de Oliveira , Gaspar Joana Margarida , de Oliveira Miguel de Abreu , Tassinari Giovanna , Cruz Luisa , Latini Alexandra
Published: February 2025

Autism Spectrum Disorder (ASD) is a neurodevelopmental condition characterized by persistent deficits in social communication and interaction, along with restricted and repetitive patterns of behavior, interests, or activities. ASD encompasses a wide spectrum of clinical presentations and functional impairments, ranging from mild to severe. Despite its prevalence, the underlying physiopathological mechanisms of ASD remain largely unknown, resulting in a lack of effective targeted therapeutic interventions, contributing to significant financial and emotional burdens on affected families and the healthcare system. Emerging evidence suggests that dysfunction in the tetrahydrobiopterin (BH4) pathway may impair the activity of monoaminergic and nitric oxide (NO)-dependent neurons in individuals with ASD. To explore this potential mechanism, we conducted a systematic review to analyze such impairments to gather information on whether the off-label use of BH4 could represent a novel pharmacological approach for managing ASD. Following the PRISMA 2020 guidelines, we systematically reviewed the literature from four databases: PubMed, Virtual Health Library, Cochrane Library, and SciELO, from January 1967 to December 2021. The quality of the included studies was assessed using the Newcastle–Ottawa scale. The inclusion criteria for this systematic review focused on identifying articles published in English that contained the following keywords, used in various combinations: autism, ASD, autism spectrum disorder, BH4, tetrahydrobiopterin, neopterin, NO, nitric oxide. The analysis was performed between December 2020 and December 2021. The collected data demonstrated that BH4 metabolism was altered in individuals with ASD. Lower levels of BH4 were reported in biological samples from ASD-affected individuals compared to age- and sex-matched controls. Additionally, neopterin levels were elevated in plasma and urine, but decreased in cerebrospinal fluid, while nitric oxide levels were consistently reported to be higher across studies. Treatment with BH4 has shown potential in improving ASD-related symptoms. The reported increase in neopterin in biological fluids indicates inflammation, while the reduction in BH4 levels suggests a potential shift in its metabolic role. Specifically, BH4 may be diverted from its primary role in neurotransmitter synthesis to function as an antioxidant or to perpetuate inflammation through NO production. Given that BH4 is a critical cofactor in monoaminergic neurotransmission, its dysfunction highlights the molecule’s therapeutic potential. BH4, already FDA-approved for other conditions, emerges as a promising off-label candidate to alleviate ASD symptomatology.
The Effects of Anger Management on Workers: A Questionnaire Survey of Organizational Dysfunctional Behavior and Withdrawal from Interpersonal Relationships in the Workplace
Authors: Semba Ryoichi
Published: February 2025

The voluntary behaviors of individuals that negatively impact the organization and its members should be addressed. According to the threatened egotism model, it is possible to curb such behaviors by effectively managing negative emotions. One such management strategy is anger management. Recently, some organizations have been implementing anger management programs, but their effect on behavior has not been verified. This study focused on organizational dysfunctional behavior and interpersonal withdrawal and attempted to examine the effects of anger management on these behaviors using a pre–post-test design. An anger management program and questionnaires before and after were administered to 92 workers (28 men and 64 women). The questionnaire included the Sociability Scale, which measures disengagement from interpersonal relationships, and the Organizational Dysfunctional Behavior Scale. A comparison of scale scores before and after the program revealed a significant decrease in the “Criticism of Others” on the Organizational Dysfunctional Behavior Scale. Furthermore, a similar analysis by sex and age revealed a significant decrease in “Criticism of Others” among women and workers under 46 years of age. These results indicate that anger management is not effective for reducing interpersonal withdrawal but may instead be effective for reducing criticism of others among women and young workers.
Academic Possible Selves, Motivational Beliefs, and Self-Regulation Among Adolescents Attending General and Vocational Schools: Does the Type of School Matter?
Authors: Efthymiadou Evropi , Gonida Eleftheria N. , Kiosseoglou Grigoris
Published: February 2025

Academic possible selves (PSs) are defined as future self-representations about education and academic outcomes, recognized for their motivational power, especially in challenging situations. This study aimed to (i) explore the salience of academic PSs among senior high school students, considering sociodemographic factors; (ii) investigate the relationships among perceived contextual factors, PS constructs, difficulty mindsets, and academic outcomes; and (iii) examine differences between general and vocational school students across these variables and their relationships. A sample of 598 10th graders (i) reported their two most important hoped-for and feared PSs as well as their strategies to achieve/avoid them and (ii) completed scales measuring demographics, perceptions about parents’ and teachers’ beliefs, perceived efficacy for attaining PSs, perception of school as a path, difficulty mindsets about academic tasks, academic self-regulation, and achievement. The results indicated that academic PSs were salient among adolescents’ hoped-for PSs, with general school students reporting academic PSs more frequently and showing more positive motivational beliefs. Path analysis indicated the role of the study variables in academic self-regulation and achievement, with school type moderating these effects. The findings emphasize the importance of supporting students’ academic PSs and tailoring context-based interventions to foster academic outcomes in diverse school settings.
Carbon Emission Assessment During the Recycling Phase of Building Meltable Materials from Construction and Demolition Waste: A Case Study in China
Authors: Jiang Boya , Huang Hao , Ge Feng , Huang Baolin , Ullah Habib
Published: February 2025

The improper disposal of construction and demolition waste (CDW) exacerbates the consumption of raw materials and emissions of greenhouse gasses. In this study, due to the high recycling rate, focusing on the meltable materials of CDW, the recycling phase of CDW is divided into four stages, namely the on-site disposal stage, the transportation stage, the reprocessing stage, and the reproduction stage. Second, based on these four stages, a carbon emission accounting model (CEAM) is established to evaluate the carbon emission benefits of meltable materials during these stages. Third, the CEAM is applied to a typical old residential area to evaluate the carbon emission reduction benefits of the CDW recycling. The results indicate that (1) the full-process carbon emissions of recycled steel, recycled flat glass, and recycled aluminum per unit mass are 677.77 kg/t, 1041.54 kg/t, and 845.39 kg/t, respectively, which are far lower than their corresponding ordinary meltable building materials (OMBMs); (2) the carbon emissions during the reproduction stage represent the primary component of carbon emissions in the MW recycling phase, accounting for 88.52% to 97.45% of the total carbon emissions; and (3) the carbon emissions generated by the recycling of cullet per unit mass are very high, reaching 1768 kg/t, which is 4.3 times that of scrap steel (409.05 kg/t) and 3.6 times that of scrap aluminum (483.76 kg/t). The research findings could provide theoretical methods and experimental data for decision-makers to formulate treatment plans for meltable materials in CDW, thereby empowering urban carbon emission reduction and promoting sustainable development. Construction parties engaged in demolition tasks should enhance on-site sorting and collaborate with recycling companies to ensure its efficient recycling. Recycling companies need to focus on high-carbon-emission stages, such as the reproduction stage, and strengthen technological research to improve carbon reduction benefits.
Strength and Stiffness of Corrugated Plates Subjected to Bending
Authors: Yoshino Yuki , Kimura Yoshihiro
Published: February 2025

When a beam twists, localized flexural deformation in the plate occurs at the connection between the corrugated plate and the beam, resulting in a reduction in the bracing stiffness of the corrugated plate. To accurately assess the bracing stiffness of a beam with a connected corrugated plate, it is essential to independently determine the bending stiffness of the corrugated plate and the rotational stiffness of the plate–beam joint. This study conducts bending experiments on corrugated plates subjected to in-plane bending to elucidate the stress mechanisms within the plates. The influence of the width-to-thickness ratio on the bending load and bending stiffness of the corrugated plates is examined using the width-to-thickness ratio regulations of various countries. The findings revealed that when the width-to-thickness ratio of the corrugated plates used in this study was lower than the threshold specified in the Eurocode, the bending load at the onset of stiffness degradation was approximately 80% of the maximum bending load, and the initial bending stiffness corresponded closely to the theoretical value. Conversely, when the web width-to-thickness ratio of the corrugated plates exceeded the limit prescribed in the Eurocode, it was demonstrated that the maximum bending load decreased to approximately 50% of the yield load, and the initial bending stiffness was reduced to about 95% of the theoretical value.
From Waste to Roads: Improving Pavement Performance and Achieving Sustainability with Recycled Steel Slag and Low-Density Polyethylene
Authors: Mehmood Syed Amir , Khan Muhammad Imran , Ahmed Sarfraz , Al-Nawasir Rania , Choudhry Rafiq M.
Published: February 2025

The use of waste, recycled, and modified materials is increasingly popular in roadway construction for sustainability and pavement longevity. This research examines the combination of steel slag (SS) and low-density polyethylene (LDPE), commonly used in plastic bags and steel manufacturing by-products, to mitigate environmental pollution. LDPE was tested as a binder modifier in two bitumen grades, 60–70 and 80–100, at concentrations of 3%, 5%, and 7% by weight. SS was used as a replacement for coarse aggregate. The physical properties of both modified and unmodified bitumen grades and SS were analyzed before creating and testing hot-mix asphalt (HMA) samples. The dynamic modulus of these samples was measured at temperatures of 4.4 °C, 21.1 °C, 37.8 °C, and 54.4 °C with frequencies of 0.1 Hz, 0.5 Hz, 1 Hz, 5 Hz, 10 Hz, and 25 Hz. Master curves were developed, and the dynamic modulus data underwent design of experiment (DOE) and computational intelligence (CI) analyses. Using KENPAVE, a mechanistic–empirical tool, the analysis assessed the design life and enhancements in damage ratio for each modifier and grade. The results showed that adding LDPE increases the softening point and penetration grade but decreases ductility due to increased bitumen stiffness, leading to premature fatigue failure at higher LDPE levels. Both 3% LDPE and 3% SS-modified LDPE improved Marshall Stability and dynamic modulus across all temperature and frequency ranges. Specifically, 3% LDPE enhanced stability by 13–16% and 3% SS-LDPE by 30–32%. The KENPAVE results for 3% LDPE showed a design life improvement of 19–25% and a damage ratio reduction of 15–18%. In comparison, 3% SS-LDPE demonstrated a design life improvement of 50–60% and a damage ratio reduction of 25–35%. Overall, this study concludes that 3% LDPE- and 3% SS-LDPE-modified HMA in both bitumen grades 60–70 and 80–100 provide optimal results for improving pavement performance.
Using One-Part Geopolymer in Stabilizing High-Water-Content Soft Clay: Towards an Eco-Friendly and Cost-Effective Solution
Authors: Li Shuo , Zhou Zihan , Hu Guo , Yao Chuanqin , Niu Fujun , Wu Jun
Published: February 2025

To achieve environmental and economic goals in ground improvement, a one-part geopolymer (OPG), synthesized from binary precursors (fly ash [FA] and granulated blast furnace slag [GGBFS]) and a solid activator (solid sodium silicate [NS]), was used to replace ordinary Portland cement (OPC) for stabilizing high-water-content soft clay. The effects of different initial water content (50%, 80%, 100%, and 120%) and various OPG binder content (10%, 20%, 30%, and 40%) on the strength development of the OPG-stabilized soft clay were investigated through unconfined compressive strength (UCS) and unconsolidated undrained (UU) triaxial tests. Additionally, the microstructure evolution and the distribution of pores in the OPG-stabilized soft clay were examined by the utilization of mercury intrusion porosimetry (MIP) and scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) techniques, respectively. The life cycle assessment (LCA) methodology was then used to analyze the environmental and economic advantages of employing an OPG binder for soil stabilization. It was revealed that the optimal content of OPG binder was contingent upon the water content of soft clay, with variations in requirements for strength development. Specifically, for soft clay not demanding early strength, a maximum binder content of 20% is proposed. Conversely, for soft clay that necessitated rapid strength gain, the OPG binder content escalated with increasing water content of the soft clay, in which soft clays with different water contents had corresponding required amounts of OPG binder. For soil with water content ranging from 50% to 80%, the recommended OPG binder content is 20%. While for soil with 100% and 120% water content, the designed OPG binder content is suggested to be 30% and 40%, respectively. The environmental assessment demonstrated that the utilization of OPG as a binder for the stabilization of soft clay reduces costs and carbon emissions in comparison to OPC. The present study provides substantial theoretical validation for the utilization of OPG as a novel binder to stabilize soft clay with elevated water content, which holds promise as an eco-friendly and cost-effective solution in ground improvement.
Curcumin and Its Derivatives in Hepatology: Therapeutic Potential and Advances in Nanoparticle Formulations
Authors: Karatayli Ersin , Sadiq Shifana C. , Schattenberg Jörn M. , Grabbe Stephan , Biersack Bernhard , Kaps Leonard
Published: February 2025

Curcumin, a plant-derived polyphenol, shows promise in hepatology for treating both malignant and non-malignant liver diseases and a subset of extrahepatic cancers. Curcumin has hepatoprotective, anti-inflammatory, antifibrotic, and antiproliferative properties, as is evident in preclinical and clinical studies. This highlights its potential as an adjunct to established cancer therapies, especially in the context of hepatocellular carcinoma and secondary liver malignancies. Curcumin also demonstrates potential in metabolic dysfunction-associated steatotic liver disease (MASLD), owing to its antifibrotic and lipid-lowering effects. However, its clinical use is limited, relating to its poor bioavailability and rapid metabolism. Nanotechnology, including liposomal and polymeric carriers, alongside synthetic curcumin derivatives, offers strategies to enhance the bioavailability and pharmacokinetic properties. We propose to revisit the use of curcumin in nanoparticle preparations in chronic liver disease and summarize current evidence in this review article.
Analysis of Prognostic Factors for Internal Carotid Artery Invasion by Nasopharyngeal Carcinoma
Authors: Lien Ching-Feng , Yeh Shyh-An , Cheng Chiu-Shih , Chiang Feng-Yu , Hwang Tzer-Zen , Cai Bi-He , Liu Chih-Yi , Weng Hsu-Huei , Chen Chia-Chi , Hsieh Meng-Che
Published: February 2025

Background: The prognostic impact of internal carotid artery (ICA) invasion in nasopharyngeal cancer (NPC) patients is not well established. Thus, we conducted a retrospective study to analyze the prognostic factors for ICA invasion by NPC. Methods: This retrospective study included consecutive biopsy-proven NPC patients who received CCRT from November 2015 to December 2022 at E-Da Hospital. Patients were then classified into two groups according to ICA invasion by tumor or not. Survival was estimated by the Kaplan–Meier method with a five-year overall survival (OS) rate and five-year disease specific survival (DSS) rate. Results: A total of 191 patients with pathologically confirmed NPC were included in this study, with 54 patients in the ICA invasion group and 137 patients in the no ICA invasion group. The ICA invasion group showed a worse prognosis compared to the no ICA invasion group (p < 0.001 in OS and DSS). Patients were stratified into a poor response group and good response group. OS and DSS in the poor response group had a significant difference compared to the good response group (both p < 0.001). In multivariate analysis, NLR was an independently prognostic factor for OS (HR 2.430, 95% CI 1.040–5.678, p = 0.040 and HR 0.412, 95% CI 0.176–0.962, p = 0.040, respectively) and for DSS (HR 2.430, 95% CI 1.040–5.678, p = 0.040 and HR 0.412, 95% CI 0.176–0.962, p = 0.040, respectively). Conclusions: Locally advanced NPC patients with ICA invasion have a miserable outcome and NLR represents a significant prognostic factor that impacts treatment decisions and survival.