bluuebunny/crossref_metadata_2025 huggingface dataset:
A Modified ABC-SQP-Based Combined Approach for the Optimization of a Parallel Hybrid Electric Vehicle
Authors: Shivappriya S. N. , Karthikeyan S. , Prabu S. , Pérez de Prado R. Pérez de , Parameshachari B. D.
Published: September 2020

In this paper, an improved fuel consumption and emissions control strategy based on a mathematical and heuristic approach is presented to optimize Parallel Hybrid Electric Vehicles (HEVs). The well-known Sequential Quadratic Programming mathematical method (SQP-Hessian approach) presents some limitations to achieve fuel consumption and emissions control optimization, as it is not able to find the global minimum, and it generally shows efficient results in local exploitation searches. The usage of a combined Modified Artificial Bee Colony algorithm (MABC) with the SQP approach is proposed in this work to obtain better optimal solutions and overcome these limitations. The optimization is performed with boundary conditions, considering that the optimized vehicle performance has to satisfy Partnership for a New Generation of Vehicles (PNGV) constraints. The weighting factor of the vehicle’s performance parameters in the objective function is varied, and optimization is carried out for two different driving cycles, namely Federal Test Procedure (FTP) and Economic commission Europe—Extra Urban Driving Cycle (ECE-EUDC), using the MABC and MABC with SQP approaches. The MABC with SQP approach shows better performance in terms of fuel consumption and emissions than the pure heuristic approach for the considered vehicle with similar boundary conditions. Moreover, it does not present significant penalties for final battery charging and it offers an optimized size of the key vehicle’s components for different driving cycles.
Feasibility Study GaN Transistors Application in the Novel Split-Coils Inductive Power Transfer System with T-Type Inverter
Authors: Shevchenko Viktor , Pakhaliuk Bohdan , Husev Oleksandr , Veligorskyi Oleksandr , Stepins Deniss , Strzelecki Ryszard
Published: September 2020

A promising solution for inductive power transfer and wireless charging is presented on the basis of a single-phase three-level T-type Neutral Point Clamped GaN-based inverter with two coupled transmitting coils. The article focuses on the feasibility study of GaN transistor application in the wireless power transfer system based on the T-type inverter on the primary side. An analysis of power losses in the main components of the system is performed: semiconductors and magnetic elements. System modeling was performed using Power Electronics Simulation Software (PSIM). It is shown that the main losses of the system are static losses in the filter inductor and rectifier diodes on the secondary side, while GaN transistors can be successfully used for the wireless power transfer system. The main features of the Printed Circuit Board (PCB) design of GaN transistors are considered in advance.
Durability of Wood Exposed to Alternating Climate Test and Natural Weathering
Authors: Stadlmann Alexander , Pramreiter Maximilian , Stingl Robert , Kurzböck Christian , Jost Thomas , Müller Ulrich
Published: September 2020

The use of wood-based materials in the automotive industry is currently under discussion and investigation. One of the major material requirements for such applications is sufficient weathering stability. This can be demonstrated by an accelerated aging process in which the samples are exposed to changing climatic conditions and a spray mist of an aqueous NaCl solution. The effects of media salt (NaCl) on the mechanical and physical properties of wood have scarcely been investigated. The presented study investigated the changes in bending strength (MOR), modulus of elasticity (MOE), and impact bending strength (α) of naturally and artificially weathered oak (Quercus spp.) and birch (Betula pendula Roth) wood. The tests provided comparable results. The decrease under natural weathering of oak was 3.73%, 4.69%, and 6.45% for MOR, MOE, and α. Under artificial weathering the decrease observed for oak was 7.33%, 10.87%, and 16.29% and 3.2%, 8.21%, and 4.03% for birch respectively. It is remarkable that α increased for birch wood at the beginning of the artificial weathering cycles. The penetration of the aqueous NaCl solution into the wood substance resulted in an increase in the wood’s equilibrium moisture content (EMC), which can be explained by the stronger hygroscopic properties of NaCl compared to wood. The higher impact strength at the beginning of artificial weathering can be partly explained by this increase in EMC. In order to investigate the penetration behavior of salt into the wood substrate, the artificially weathered samples were examined by means of energy dispersive X-ray analysis (EDX) and it was shown that the salt concentration changes significantly over the weathering cycles and sample cross-section.
An Automatic Method for Stomatal Pore Detection and Measurement in Microscope Images of Plant Leaf Based on a Convolutional Neural Network Model
Authors: Song Wenlong , Li Junyu , Li Kexin , Chen Jingxu , Huang Jianping
Published: September 2020

Stomata are microscopic pores on the plant epidermis that regulate the water content and CO2 levels in leaves. Thus, they play an important role in plant growth and development. Currently, most of the common methods for the measurement of pore anatomy parameters involve manual measurement or semi-automatic analysis technology, which makes it difficult to achieve high-throughput and automated processing. This paper presents a method for the automatic segmentation and parameter calculation of stomatal pores in microscope images of plant leaves based on deep convolutional neural networks. The proposed method uses a type of convolutional neural network model (Mask R-CNN (region-based convolutional neural network)) to obtain the contour coordinates of the pore regions in microscope images of leaves. The anatomy parameters of pores are then obtained by ellipse fitting technology, and the quantitative analysis of pore parameters is implemented. Stomatal microscope image datasets for black poplar leaves were obtained using a large depth-of-field microscope observation system, the VHX-2000, from Keyence Corporation. The images used in the training, validation, and test sets were taken randomly from the datasets (562, 188, and 188 images, respectively). After 10-fold cross validation, the 188 test images were found to contain an average of 2278 pores (pore widths smaller than 0.34 μm (1.65 pixels) were considered to be closed stomata), and an average of 2201 pores were detected by our network with a detection accuracy of 96.6%, and the intersection of union (IoU) of the pores was 0.82. The segmentation results of 2201 stomatal pores of black poplar leaves showed that the average measurement accuracies of the (a) pore length, (b) pore width, (c) area, (d) eccentricity, and (e) degree of stomatal opening, with a ratio of width-to-maximum length of a stomatal pore, were (a) 94.66%, (b) 93.54%, (c) 90.73%, (d) 99.09%, and (e) 92.95%, respectively. The proposed stomatal pore detection and measurement method based on the Mask R-CNN can automatically measure the anatomy parameters of pores in plants, thus helping researchers to obtain accurate stomatal pore information for leaves in an efficient and simple way.
Fatty Acids and Stable Isotope Ratios in Shiitake Mushrooms (Lentinula edodes) Indicate the Origin of the Cultivation Substrate Used: A Preliminary Case Study in Korea
Authors: Chung Ill-Min , Kim So-Yeon , Han Jae-Gu , Kong Won-Sik , Jung Mun Yhung , Kim Seung-Hyun
Published: September 2020

Shiitake mushroom (Lentinula edodes) is commonly consumed worldwide and is cultivated in many farms in Korea using Chinese substrates owing to a lack of knowledge on how to prepare sawdust-based substrate blocks (bag cultivation). Consequently, issues related to the origin of the Korean or Chinese substrate used in shiitake mushrooms produced using bag cultivation have been reported. Here, we investigated differences in fatty acids (FAs) and stable isotope ratios (SIRs) in shiitake mushrooms cultivated using Korean and Chinese substrates under similar conditions (strain, temperature, humidity, etc.) and depending on the harvesting cycle. The total FA level decreased significantly by 5.49 mg∙g−1 as the harvesting cycle increased (p < 0.0001); however, no differences were found in FAs between shiitake mushrooms cultivated using Korean and Chinese substrates. Linoleic acid was the most abundant FA, accounting for 77–81% of the total FAs during four harvesting cycles. Moreover, the SIRs differed significantly between the Korean and Chinese substrates, and the harvesting cycles resulted in smaller maximum differences in SIR values compared to those of the cultivation substrate origins. Our findings contribute to the identification of the geographical origin of shiitake mushrooms and may have potential applications in international shiitake-mushroom markets.
Growth Potential of Listeria monocytogenes on Refrigerated Spinach and Rocket Leaves in Modified Atmosphere Packaging
Authors: Culliney Paul , Schmalenberger Achim
Published: September 2020

Minimally processed ready-to-eat (RTE) vegetables are increasingly consumed for their health benefits. However, they also pose a risk of being ingested with food-borne pathogens. The present study investigated the ability of RTE spinach and rocket to support the growth of Listeria monocytogenes as previous studies provided contradicting evidence. Findings were compared to growth on iceberg lettuce that has repeatedly been shown to support growth. Products were inoculated with a three-strain mix of L. monocytogenes at 10 and 100 cfu g−1 and stored in modified atmosphere (4 kPa O2, 8 kPa CO2) at 8 °C over 7–9 days. Spinach demonstrated the highest growth potential rate of 2 to 3 log10 cfu g−1 over a 9-day period with only marginal deterioration in its visual appearance. Growth potential on rocket was around 2 log10 cfu g−1 over 9 days with considerable deterioration in visual appearance. Growth potential of iceberg lettuce was similar to that of rocket over a 7-day period. Growth curves fitted closely to a linear growth model, indicating none to limited restrictions of growth over the duration of storage. The high growth potentials of L. monocytogenes on spinach alongside the limited visual deterioration highlight the potential risks of consuming this raw RTE food product when contaminated.
Eliciting Egg Consumer Preferences for Organic Labels and Omega 3 Claims in Italy and Hungary
Authors: Yeh Ching-Hua , Menozzi Davide , Török Áron
Published: September 2020

This paper investigates consumers’ preferences for egg purchase in two European countries, Hungary and Italy. We utilize random parameter logit models to interpret the results of discrete choice experiments (DCE) for the elicitation of preference of the egg consumers. A sample of 403 in the Hungarian survey and 404 in the Italian survey were recruited in summer 2018. The DCE questionnaire includes the following product and process characteristics: organic labels, nutrition and health claims, and price. Our results show that for Hungarian and Italian consumers, the price is the most important attribute, followed by the nutrition and health claim and the organic production labelling. Three egg consumer segments can be identified via latent class models for each country. In both countries, we found similar consumer groups, the Price Sensitive and Quality Optimizing Opportunist Consumers and Health Conscious Buyers, respectively. Particularly, compared to the other segments the Health Conscious Buyers (46% in Hungary and 49% in Italy) exhibited stronger preference for and are willing to pay a higher price premium for eggs with organic label and nutrition claims. In Italy, we identified a third segment with consumers preferring simpler labelling approach, whilst in Hungary we found a consumer segment distrusting the EU organic logo.
Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence
Authors: Kiranmayee K. N. S. Usha , Hash C. Tom , Sivasubramani S. , Ramu P. , Amindala Bhanu Prakash , Rathore Abhishek , Kishor P. B. Kavi , Gupta Rajeev , Deshpande Santosh P.
Published: September 2020

This study was conducted to dissect the genetic basis and to explore the candidate genes underlying one of the important genomic regions on an SBI-10 long arm (L), governing the complex stay-green trait contributing to post-flowering drought-tolerance in sorghum. A fine-mapping population was developed from an introgression line cross—RSG04008-6 (stay-green) × J2614-11 (moderately senescent). The fine-mapping population with 1894 F2 was genotyped with eight SSRs and a set of 152 recombinants was identified, advanced to the F4 generation, field evaluated with three replications over 2 seasons, and genotyped with the GBS approach. A high-resolution linkage map was developed for SBI-10L using 260 genotyping by sequencing—Single Nucleotide Polymorphism (GBS–SNPs). Using the best linear unpredicted means (BLUPs) of the percent green leaf area (%GL) traits and the GBS-based SNPs, we identified seven quantitative trait loci (QTL) clusters and single gene, mostly involved in drought-tolerance, for each QTL cluster, viz., AP2/ERF transcription factor family (Sobic.010G202700), NBS-LRR protein (Sobic.010G205600), ankyrin-repeat protein (Sobic.010G205800), senescence-associated protein (Sobic.010G270300), WD40 (Sobic.010G205900), CPK1 adapter protein (Sobic.010G264400), LEA2 protein (Sobic.010G259200) and an expressed protein (Sobic.010G201100). The target genomic region was thus delimited from 15 Mb to 8 genes co-localized with QTL clusters, and validated using quantitative real-time (qRT)–PCR.
A Highly Polymorphic Panel Consisting of Microhaplotypes and Compound Markers with the NGS and Its Forensic Efficiency Evaluations in Chinese Two Groups
Authors: Jin Xiaoye , Zhang Xingru , Shen Chunmei , Liu Yanfang , Cui Wei , Chen Chong , Guo Yuxin , Zhu Bofeng
Published: September 2020

Novel genetic markers like microhaplotypes and compound markers show promising potential in forensic research. Based on previously reported single nucleotide polymorphism (SNP) and insertion/deletion (InDel) polymorphism loci, 29 genetic markers including 22 microhaplotypes and seven compound markers were identified. Genetic distributions of the 29 loci in five continental populations, Kazak and Mongolian groups in China were investigated. We found that the expected heterozygosity values of these 29 loci were >0.4 in these populations, indicating these loci were relatively high polymorphisms. Population genetic analyses of five continental populations showed that five loci displayed relatively high genetic variations among these continental populations and could be useful markers for ancestry analysis. In summary, the 29 loci displayed relatively high genetic diversities in continental populations and Chinese two groups and could be informative loci for forensic research.
COVID-19 Pandemic: Perspective of an Italian Tertiary Care Pediatric Center
Authors: Donà Daniele , Giaquinto Carlo , Baraldi Eugenio , Biffi Alessandra , Gamba Piergiorgio , Saieva Anna Maria , Antoniello Luca , Costenaro Paola , Masiero Susanna , Sainati Laura , Da Dalt Liviana , Perilongo Giorgio
Published: September 2020

Since February 2020, Italy has been faced with the dramatic spread of novel Coronavirus SARS-CoV-2. This impetuous pandemic infection forced many hospitals to reorganize their healthcare systems. Predicting a rapid spread of the SARS-CoV-2 virus within our region, the Department for Women’s and Children’s Health promptly decided (i) to revise the distribution of the clinical areas in order to create both designated COVID-19 and COVID-19-free areas with their own access, (ii) to reinforce infection prevention control (IPC) measures for all healthcare workers and administrative staff and (iii) to adopt the new “double-gate approach”: a phone call pre-triage and nasopharyngeal swab for SARS-CoV-2 detection before the admission of all patients and caregivers. Between 21 February 2020 till 04 May 2020, only seven physicians, two nurses and two of the administrative staff resulted positive, all during the first week of March. No other cases of intra-department infection were documented among the healthcare workers since all the preventive procedures described above were implemented. It is predicted that similar situations can happen again in the future, and thus, it is necessary to be more prepared to deal with them than we were at the beginning of this COVID-19 pandemic.
The Relationship between Poor Pulmonary Function and Irregular Pulse in the Elderly: Findings from a Nationwide Cross-Sectional Survey in Korea
Authors: Kim Sun Hwa , Lee Yonggu , Hwang Seon Young , Shin Jinho , Kim Chun Ki , Park Jin-Kyu
Published: September 2020

Arrhythmia may be caused by reduced pulmonary function, and pulse palpation is a useful screening method for the early detection of cardiac arrhythmia. The aim of this study was to investigate the association between reduced pulmonary function and abnormal findings on pulse palpation in 2347 subjects aged ≥65 years using data from a nationwide survey. Pulse palpation was initially performed for 15 s and, if felt to be abnormal, it was performed again for 60 s. The prevalence of irregular pulse (IP) determined by the 60-second palpation was 61 (2.6%). The mean age of subjects with an IP was 73.0 (95% CI 71.7–74.3) years, and 45.8% were male. After adjustment for covariates, forced vital capacity (FVC)/predicted FVC, forced expiratory volume in one second (FEV1)/predicted FEV1, and the lowest FEV1 remained significant risk factors for IP. A restrictive or obstructive spirometry pattern was also an independent risk factor for IP. In summary, an IP is more prevalent when pulmonary function is reduced in the elderly, in whom careful pulse palpation may be necessary for the early detection of arrhythmia.
Revealing Cryptic Changes of Cyanobacterial Community Structure in Two Eutrophic Lakes Using eDNA Sequencing
Authors: Jiang Yongguang , Xiao Peng , Yu Gongliang , Song Gaofei , Li Renhui
Published: September 2020

Harmful cyanobacterial blooms pose a risk to human health worldwide. To enhance understanding on the bloom-forming mechanism, the spatiotemporal changes in cyanobacterial diversity and composition in two eutrophic lakes (Erhai Lake and Lushui Reservoir) of China were investigated from 2010 to 2011 by high-throughput sequencing of environmental DNA. For each sample, 118 to 260 cpcBA-IGS operational taxonomic units (OTUs) were obtained. Fifty-two abundant OTUs were identified, which made up 95.2% of the total sequences and were clustered into nine cyanobacterial groups. Although the cyanobacterial communities of both lakes were mainly dominated by Microcystis, Erhai Lake had a higher cyanobacterial diversity. The abundance of mixed Nostocales species was lower than that of Microcystis, whereas Phormidium and Synechococcus were opportunistically dominant. The correlation between the occurrence frequency and relative abundance of OTUs was poorly fitted by the Sloan neutral model. Deterministic processes such as phosphorus availability were shown to have significant effects on the cyanobacterial community structure in Erhai Lake. In summary, the Microcystis-dominated cyanobacterial community was mainly affected by the deterministic process. Opportunistically dominant species have the potential to replace Microcystis and form blooms in eutrophic lakes, indicating the necessity to monitor these species for drinking water safety.
How Spiritual Leadership Boosts Nurses’ Work Engagement: The Mediating Roles of Calling and Psychological Capital
Authors: Wu Wei-Li , Lee Yi-Chih
Published: September 2020

Work engagement is an important topic in the field of nursing management. Meanwhile, spiritual leadership has been demonstrated to have a positive impact on healthcare workers. However, the relationship between spiritual leadership and work engagement is unclear. The main purpose of this study was to investigate the influence of spiritual leadership on work engagement through increased spiritual well-being and psychological capital. This study used a cross-sectional survey to collect data in Taiwan. The sample included 164 nurses, with empirical testing carried out by PROCESS Macro for SPSS. The results show that spiritual leadership has a positive influence on work engagement and that spiritual well-being (i.e., calling) and psychological capital mediate the effect of spiritual leadership on work engagement. According to the results of this study, nursing leaders must be aware of the role of spiritual leadership in promoting work engagement.
Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s
Authors: Pang Yanhong , Kovachev Petar , Sanyal Suparna
Published: September 2020

The role of the nucleic acids in prion aggregation/disaggregation is becoming more and more evident. Here, using HET-s prion from fungi Podospora anserina (P. anserina) as a model system, we studied the role of RNA, particularly of different domains of the ribosomal RNA (rRNA), in its aggregation process. Our results using Rayleigh light scattering, Thioflavin T (ThT) binding, transmission electron microscopy (TEM) and cross-seeding assay show that rRNA, in particular the domain V of the major rRNA from the large subunit of the ribosome, substantially prevents insoluble amyloid and amorphous aggregation of the HET-s prion in a concentration-dependent manner. Instead, it facilitates the formation of the soluble oligomeric “seeds”, which are capable of promoting de novo HET-s aggregation. The sites of interactions of the HET-s prion protein on domain V rRNA were identified by primer extension analysis followed by UV-crosslinking, which overlap with the sites previously identified for the protein-folding activity of the ribosome (PFAR). This study clarifies a missing link between the rRNA-based PFAR and the mode of propagation of the fungal prions.
Genomics and Functional Genomics of Malignant Pleural Mesothelioma
Authors: Cakiroglu Ece , Senturk Serif
Published: September 2020

Malignant pleural mesothelioma (MPM) is a rare, aggressive cancer of the mesothelial cells lining the pleural surface of the chest wall and lung. The etiology of MPM is strongly associated with prior exposure to asbestos fibers, and the median survival rate of the diagnosed patients is approximately one year. Despite the latest advancements in surgical techniques and systemic therapies, currently available treatment modalities of MPM fail to provide long-term survival. The increasing incidence of MPM highlights the need for finding effective treatments. Targeted therapies offer personalized treatments in many cancers. However, targeted therapy in MPM is not recommended by clinical guidelines mainly because of poor target definition. A better understanding of the molecular and cellular mechanisms and the predictors of poor clinical outcomes of MPM is required to identify novel targets and develop precise and effective treatments. Recent advances in the genomics and functional genomics fields have provided groundbreaking insights into the genomic and molecular profiles of MPM and enabled the functional characterization of the genetic alterations. This review provides a comprehensive overview of the relevant literature and highlights the potential of state-of-the-art genomics and functional genomics research to facilitate the development of novel diagnostics and therapeutic modalities in MPM.
5-(N-Trifluoromethylcarboxy)aminouracil as a Potential DNA Radiosensitizer and Its Radiochemical Conversion into N-Uracil-5-yloxamic Acid
Authors: Spisz Paulina , Kozak Witold , Chomicz-Mańka Lidia , Makurat Samanta , Falkiewicz Karina , Sikorski Artur , Czaja Anna , Rak Janusz , Zdrowowicz Magdalena
Published: September 2020

Hypoxia—a hallmark of solid tumors—dramatically impairs radiotherapy, one of the most common anticancer modalities. The adverse effect of the low-oxygen state can be eliminated by the concomitant use of a hypoxic cell radiosensitizer. In the present paper, we show that 5-(N-trifluoromethylcarboxy) aminouracil (CF3CONHU) can be considered as an effective radiosensitizer of DNA damage, working under hypoxia. The title compound was synthesized in the reaction of 5-aminouracil and trifluoroacetic anhydride in trifluoroacetic acid. Then, an aqueous and deoxygenated solution of the HPLC purified compound containing tert-butanol as a hydroxyl radical scavenger was irradiated with X-rays. Radiodegradation in a 26.67 ± 0.31% yield resulted in only one major product—N-uracil-5-yloxamic acid. The mechanism that is possibly responsible for the formation of the observed radioproduct has been elucidated with the use of DFT calculations. The cytotoxic test against the PC3 prostate cancer cell line and HDFa human dermal fibroblasts confirmed the low cytotoxicity of CF3CONHU. Finally, a clonogenic assay and flow cytometric analysis of histone H2A.X phosphorylation proved the radiosensitization in vitro.
Modeling the Paraphrase Detection Task over a Heterogeneous Graph Network with Data Augmentation
Authors: Anchiêta Rafael T. , Sousa Rogério F. de , Pardo Thiago A. S.
Published: September 2020

Paraphrase detection is a Natural-Language Processing (NLP) task that aims at automatically identifying whether two sentences convey the same meaning (even with different words). For the Portuguese language, most of the works model this task as a machine-learning solution, extracting features and training a classifier. In this paper, following a different line, we explore a graph structure representation and model the paraphrase identification task over a heterogeneous network. We also adopt a back-translation strategy for data augmentation to balance the dataset we use. Our approach, although simple, outperforms the best results reported for the paraphrase detection task in Portuguese, showing that graph structures may capture better the semantic relatedness among sentences.
Effects of Three Artificial Diets on Life History Parameters of the Ladybird Beetle Stethorus gilvifrons, a Predator of Tetranychid Mites
Authors: Ebrahimifar Jafar , Shishehbor Parviz , Rasekh Arash , Hemmati Seyed Ali , Riddick Eric W.
Published: September 2020

Background: The ladybird beetle Stethorus gilvifrons (Mulsant) is an important natural enemy of tetranychid mites and functions as a biological control of these plant pests. The development, survival and reproduction of S. gilvifrons were studied when fed on three artificial diets. The components of the artificial diet that S. gilvifrons could be reared successfully on for one generation with no use of tetranychid mites were examined. Methods: Artificial diets consisted of sucrose, honey, royal jelly, agar, yeast, date palm pollen supplemented in different diets with hen’s egg yolk (AD1, as basic diet), Ephestia kuehniella Zeller eggs (AD2), or E. kuehniella eggs and 2,4-dihydroxybenzoic acid (AD3). Results: Adults and larvae of Stethorusgilvifrons fed on AD1 had a shorter immature development and preoviposition periods than those fed on AD2 and AD3. The total number of deposited eggs was significantly higher for the females fed on AD3 than on the other diets. The intrinsic rate of increase (r) of S. gilvifrons was highest on AD3, followed by AD2, and AD1. Conclusion: Stethorus gilvifrons performed best on AD3, indicating the potential of this artificial diet for the mass rearing of this important predatory ladybird beetle.
Proteomics of the Honeydew from the Brown Planthopper and Green Rice Leafhopper Reveal They Are Rich in Proteins from Insects, Rice Plant and Bacteria
Authors: Zhu Jinghua , Zhu Kunmiao , Li Liang , Li Zengxin , Qin Weiwei , Park Yoonseong , He Yueping
Published: September 2020

Honeydew is a watery fluid excreted by plant sap-feeding insects. It is a waste product for the insect hosts. However, it plays important roles for other organisms, such as serving as a nutritional source for beneficial insects and bacteria, as well as elicitors and effectors modulating plant responses. In this study, shotgun LC–MS/MS analyses were used to identify the proteins in the honeydew from two important rice hemipteran pests, the brown planthopper (Nilaparvata lugens, BPH) and green rice leafhopper (Nephotettix cincticeps, GRH). A total of 277 and 210 proteins annotated to insect proteins were identified in the BPH and GRH honeydews, respectively. These included saliva proteins that may have similar functions as the saliva proteins, such as calcium-binding proteins and apolipophorin, involved in rice plant defenses. Additionally, a total of 52 and 32 Oryza proteins were identified in the BPH and GRH honeydews, respectively, some of which are involved in the plant immune system, such as Pathogen-Related Protein 10, ascorbate peroxidase, thioredoxin and glutaredoxin. Coincidently, 570 and 494 bacteria proteins were identified from the BPH and GRH honeydews, respectively, which included several well-known proteins involved in the plant immune system: elongation factor Tu, flagellin, GroEL and cold-shock proteins. The results of our study indicate that the insect honeydew is a complex fluid cocktail that contains abundant proteins from insects, plants and microbes, which may be involved in the multitrophic interactions of plants–insects–microbes.
Strong Gene Flow Undermines Local Adaptations in a Host Parasite System
Authors: Seppä Perttu , Bonelli Mariaelena , Dupont Simon , Hakala Sanja Maria , Bagnères Anne-Geneviève , Lorenzi Maria Cristina
Published: September 2020

The co-evolutionary pathways followed by hosts and parasites strongly depend on the adaptive potential of antagonists and its underlying genetic architecture. Geographically structured populations of interacting species often experience local differences in the strength of reciprocal selection pressures, which can result in a geographic mosaic of co-evolution. One example of such a system is the boreo-montane social wasp Polistes biglumis and its social parasite Polistes atrimandibularis, which have evolved local defense and counter-defense mechanisms to match their antagonist. In this work, we study spatial genetic structure of P. biglumis and P. atrimandibularis populations at local and regional scales in the Alps, by using nuclear markers (DNA microsatellites, AFLP) and mitochondrial sequences. Both the host and the parasite populations harbored similar amounts of genetic variation. Host populations were not genetically structured at the local scale, but geographic regions were significantly differentiated from each other in both the host and the parasite in all markers. The net dispersal inferred from genetic differentiation was similar in the host and the parasite, which may be due to the annual migration pattern of the parasites between alpine and lowland populations. Thus, the apparent dispersal barriers (i.e., high mountains) do not restrict gene flow as expected and there are no important gene flow differences between the species, which contradict the hypothesis that restricted gene flow is required for local adaptations to evolve.
Genetic Parameters of Honey Bee Colonies Traits in a Canadian Selection Program
Authors: Maucourt Ségolène , Fortin Frédéric , Robert Claude , Giovenazzo Pierre
Published: September 2020

Genetic selection has led to spectacular advances in animal production in many domestic species. However, it is still little applied to honey bees (Apis mellifera), whose complex genetic and reproductive characteristics are a challenge to model statistically. Advances in informatics now enable creation of a statistical model consistent with honey bee genetics, and, consequently, genetic selection for this species. The aim of this project was to determine the genetic parameters of several traits important for Canadian beekeepers with a view to establishing a breeding program in a northern context. Our results show that the five traits measured (Varroa destructor infestation, spring development, honey production, winter consumption, and hygienic behavior) are heritable. Thus, the rate of V. destructor infestation has a high heritability (h2 = 0.44 ± 0.56), spring development and honey production have a medium heritability (respectively, h2 = 0.30 ± 0.14 and h2 = 0.20 ± 0.13), and winter consumption and hygienic behavior have a low heritability (respectively, h2 = 0.11 ± 0.09 and h2 = 0.18 ± 0.13). Furthermore, the genetic correlations between these traits are all positive or null, except between hygienic behavior and V. destructor infestation level. These genetic parameters will be instrumental to the development of a selection index that will be used to improve the capacity of honey bees to thrive in northern conditions.
Compatibility of the Predatory Beetle, Delphastus catalinae, with an Entomopathogenic Fungus, Cordyceps fumosorosea, for Biocontrol of Invasive Pepper Whitefly, Aleurothrixus trachoides, in Florida
Authors: Avery Pasco B. , Kumar Vivek , Francis Antonio , McKenzie Cindy L. , Osborne Lance S.
Published: September 2020

Aleurothrixus (formerly known as Aleurotrachelus) trachoides Back (Hemiptera: Aleyrodidae), commonly known as pepper or solanum whitefly, is a new emerging whitefly pest of several horticultural crops in the United States. During the preliminary survey for pepper whitefly infestation in Florida, a whitefly-specific predatory beetle Delphastus catalinae Horn (Coleoptera: Coccinellidae) was observed associated with this pest in the natural ecosystem. The current study was undertaken to determine the efficacy of this naturally occurring predator, D. catalinae, and an entomopathogenic fungus, Cordyceps (formerly known as Isaria) fumosorosea (Cfr) (Wize) (Hypocreales: Cordycipitaceae), alone or in combination, under controlled laboratory conditions. Whitefly mortality for Cfr (88%), beetle (100%) and Cfr + beetle (100%) treatments were similar and significantly higher compared to control. In the combination treatment, Cfr did not impact D. catalinae longevity and daily food intake, indicating a neutral interaction between the two treatments. Results showed that both of these natural biological control agents could potentially offer an effective alternative in the battle against invasive whiteflies such as pepper whitefly in U.S. horticulture production, either as a stand-alone strategy or in an integrated approach. Although these findings are promising, compatibility of the two treatments needs to be evaluated further under greenhouse and semi-field conditions before recommending to commercial growers.
A Regional Operational Model for the North East Atlantic: Model Configuration and Validation
Authors: Nagy Hazem , Lyons Kieran , Nolan Glenn , Cure Marcel , Dabrowski Tomasz
Published: September 2020

An operational model for an area of the northeast Atlantic that encompasses all of Ireland’s territorial waters has been developed. The model is an implementation of the Regional Ocean Modelling System (ROMS) and uses operationally available atmospheric and boundary forcing, and a global tide solution for tidal forcing. River forcing is provided by climatological daily discharge rates for 29 rivers across Ireland, west Britain, and west France. It is run in an operational framework to produce 7-day hindcasts once a week, and daily 3-day forecasts which are published in a number of formats. We evaluated the model skill by comparing with measured data and calculating statistics such as mean error, root mean square error (RMSE), and correlation coefficient. The observations consist of satellite Sea Surface Temperature (SST), total surface velocity fields from satellite, water level time series from around the Irish coast, and temperature and salinity data from Array for Real-Time Geostrophic Oceanography (ARGO) and Conductivity Temperature Depth (CTD) profiles. The validation period is from 1 January 2016 until 31 December 2019. The correlation coefficient between the model and satellite SST is 0.97 and recorded in March and April 2018. The model error is about 5% of the total M2 amplitude in the Celtic Sea recorded at Dunmore East tide gauge station. The maximum RMSE between the model and the CTD temperature profiles is 0.8 °C while it is 0.17 PSU for salinity. The model correctly defines the shelf water masses around Ireland. In 2019 the Irish Coastal Current (ICC) was very strong and well defined along most of the western Irish coast. The model results have well reproduced the ICC front for the whole simulation period.
Sestrin2 Expression Has Regulatory Properties and Prognostic Value in Lung Cancer
Authors: Chae Hee Sung , Gil Minchan , Saha Subbroto Kumar , Kwak Hee Jeung , Park Hwan-Woo , Vellingiri Balachandar , Cho Ssang-Goo
Published: September 2020

Lung cancer remains the most dangerous type of cancer despite recent progress in therapeutic modalities. Development of prognostic markers and therapeutic targets is necessary to enhance lung cancer patient survival. Sestrin family genes (Sestrin1, Sestrin2, and Sestrin3) are involved in protecting cells from stress. In particular, Sestrin2, which mainly protects cells from oxidative stress and acts as a leucine sensor protein in mammalian target of rapamycin (mTOR) signaling, is thought to affect various cancers in different ways. To investigate the role of Sestrin2 expression in lung cancer cells, we knocked down Sestrin2 in A549, a non-small cell lung cancer cell line; this resulted in reduced cell proliferation, migration, sphere formation, and drug resistance, suggesting that Sestrin2 is closely related to lung cancer progression. We analyzed Sestrin2 expression in human tissue using various bioinformatic databases and confirmed higher expression of Sestrin2 in lung cancer cells than in normal lung cells using Oncomine and the Human Protein Atlas. Moreover, analyses using Prognoscan and KMplotter showed that Sestrin2 expression is negatively correlated with the survival of lung cancer patients in multiple datasets. Co-expressed gene analysis revealed Sestrin2-regulated genes and possible associated pathways. Overall, these data suggest that Sestrin2 expression has prognostic value and that it is a possible therapeutic target in lung cancer.
2,4,6-Trimethylbenzyl Chloride (α2-Chloroisodurene)
Authors: Aitken R. Alan , Slawin Alexandra M. Z.
Published: September 2020

The X-ray structure of the title compound has been determined and it is compared with those of other substituted benzyl chlorides reported previously. It has an atypically long CH2–Cl bond.
Effect of Process Parameters on Tensile Mechanical Properties of 3D Printing Continuous Carbon Fiber-Reinforced PLA Composites
Authors: Dou Hao , Cheng Yunyong , Ye Wenguang , Zhang Dinghua , Li Junjie , Miao Zhoujun , Rudykh Stephan
Published: September 2020

Three-dimensional (3D) printing continuous carbon fiber-reinforced polylactic acid (PLA) composites offer excellent tensile mechanical properties. The present study aimed to research the effect of process parameters on the tensile mechanical properties of 3D printing composite specimens through a series of mechanical experiments. The main printing parameters, including layer height, extrusion width, printing temperature, and printing speed are changed to manufacture specimens based on the modified fused filament fabrication 3D printer, and the tensile mechanical properties of 3D printing continuous carbon fiber-reinforced PLA composites are presented. By comparing the outcomes of experiments, the results show that relative fiber content has a significant impact on mechanical properties and the ratio of carbon fibers in composites is influenced by layer height and extrusion width. The tensile mechanical properties of continuous carbon fiber-reinforced composites gradually decrease with an increase of layer height and extrusion width. In addition, printing temperature and speed also affect the fiber matrix interface, i.e., tensile mechanical properties increase as the printing temperature rises, while the tensile mechanical properties decrease when the printing speed increases. Furthermore, the strengthening mechanism on the tensile mechanical properties is that external loads subjected to the components can be transferred to the carbon fibers through the fiber-matrix interface. Additionally, SEM images suggest that the main weakness of continuous carbon fiber-reinforced 3D printing composites exists in the fiber-matrix interface, and the main failure is the pull-out of the fiber caused by the interface destruction.
Poly-Epsilon-Lysine Hydrogels with Dynamic Crosslinking Facilitates Cell Proliferation
Authors: Lopez Mora Nestor , Owens Matthew , Schmidt Sara , Silva Andreia F. , Bradley Mark
Published: September 2020

The extracellular matrix (ECM) is a three-dimensional network within which fundamental cell processes such as cell attachment, proliferation, and differentiation occur driven by its inherent biological and structural cues. Hydrogels have been used as biomaterials as they possess many of the ECM characteristics that control cellular processes. However, the permanent crosslinking often found in hydrogels fails to recapitulate the dynamic nature of the natural ECM. This not only hinders natural cellular migration but must also limit cellular expansion and growth. Moreover, there is an increased interest in the use of new biopolymers to create biomimetic materials that can be used for biomedical applications. Here we report on the natural polymer poly-ε-lysine in forming dynamic hydrogels via reversible imine bond formation, with cell attachment promoted by arginine-glycine-aspartic acid (RGD) incorporation. Together, the mechanical properties and cell behavior of the dynamic hydrogels with low poly-ε-lysine quantities indicated good cell viability and high metabolic activity.
Electrospinning of PCL-Based Blends: Processing Optimization for Their Scalable Production
Authors: Arrieta Marina P. , Leonés Gil Adrián , Yusef Maysa , Kenny José M. , Peponi Laura
Published: September 2020

In this work poly(ε-caprolactone) (PCL) based electrospun mats were prepared by blending PCL with microcrystalline cellulose (MCC) and poly(3-hydroxybutyrate) (PHB). The electrospinning processing parameters were firstly optimized with the aim to obtain scalable PCL-based electrospun mats to be used in the industrial sector. Neat PCL as well as PCL-MCC and PCL-PHB based mats in different proportions (99:1; 95:5; 90:10) were prepared. A complete morphological, thermal and mechanical characterization of the developed materials was carried out. Scanning electron microscopy (SEM) observations showed that the addition of PHB to the PCL matrix considerably reduced the formation of beads. Both the addition of MCC and PHB reduced the thermal stability of PCL, but obtained materials with enough thermal stability for the intended use. The electrospun PCL fibers show greatly reduced flexibility with respect to the PCL bulk material, however when PCL is blended with PHB their stretchability is increased, changing their elongation at break from 35% to 70% when 10 wt% of PHB is blended with PCL. However, the mechanical response of the different blends increases with respect to the neat electrospun PCL, offering the possibility to modulate their properties according to the required industrial applications.
The Non-Smooth and Bi-Objective Team Orienteering Problem with Soft Constraints
Authors: Estrada-Moreno Alejandro , Ferrer Albert , Juan Angel A. , Panadero Javier , Bagirov Adil
Published: September 2020

In the classical team orienteering problem (TOP), a fixed fleet of vehicles is employed, each of them with a limited driving range. The manager has to decide about the subset of customers to visit, as well as the visiting order (routes). Each customer offers a different reward, which is gathered the first time that it is visited. The goal is then to maximize the total reward collected without exceeding the driving range constraint. This paper analyzes a more realistic version of the TOP in which the driving range limitation is considered as a soft constraint: every time that this range is exceeded, a penalty cost is triggered. This cost is modeled as a piece-wise function, which depends on factors such as the distance of the vehicle to the destination depot. As a result, the traditional reward-maximization objective becomes a non-smooth function. In addition, a second objective, regarding the design of balanced routing plans, is considered as well. A mathematical model for this non-smooth and bi-objective TOP is provided, and a biased-randomized algorithm is proposed as a solving approach.
Normal Toeplitz Operators on the Bergman Space
Authors: Kim Sumin , Lee Jongrak
Published: September 2020

In this paper, we give a characterization of normality of Toeplitz operator Tφ on the Bergman space A2(D). First, we state basic properties for Toeplitz operator Tφ on A2(D). Next, we consider the normal Toeplitz operator Tφ on A2(D) in terms of harmonic symbols φ. Finally, we characterize the normal Toeplitz operators Tφ with non-harmonic symbols acting on A2(D).
A Review on the Qualitative Behavior of Solutions in Some Chemotaxis–Haptotaxis Models of Cancer Invasion
Authors: Wang Yifu
Published: September 2020

Chemotaxis is an oriented movement of cells and organisms in response to chemical signals, and plays an important role in the life of many cells and microorganisms, such as the transport of embryonic cells to developing tissues and immune cells to infection sites. Since the pioneering works of Keller and Segel, there has been a great deal of literature on the qualitative analysis of chemotaxis systems. As an important extension of the Keller–Segel system, a variety of chemotaxis–haptotaxis models have been proposed in order to gain a comprehensive understanding of the invasion–metastasis cascade. From a mathematical point of view, the rigorous analysis thereof is a nontrivial issue due to the fact that partial differential equations (PDEs) for the quantities on the macroscale are strongly coupled with ordinary differential equations (ODEs) modeling the subcellular events. It is the goal of this paper to describe recent results of some chemotaxis–haptotaxis models, inter alia macro cancer invasion models proposed by Chaplain et al., and multiscale cancer invasion models by Stinner et al., and also to introduce some open problems.
A Didactic Procedure to Solve the Equation of Steady-Static Response in Suspended Cables
Authors: Agüero-Rubio José , López-Martínez Javier , Gómez-Galán Marta , Callejón-Ferre Ángel-Jesús
Published: September 2020

Students in the electrical branch of the short-cycle tertiary education program acquire developmental and design skills for low voltage transmission power lines. Aerial power line design requires mathematical tools not covered well enough in the curricula. Designing suspension cables requires the use of a Taylor series and integral calculation to obtain the parabola’s arc length. Moreover, it requires iterative procedures, such as the Newton–Raphson method, to solve the third-order equation of the steady-static response. The aim of this work is to solve the steady-static response equation for suspended cables using simple calculation tools. For this purpose, the influence of the horizontal component of the cable tension on its curvature was decoupled from the cable’s self-weight, which was responsible for the tension’s vertical component. To this end, we analyzed the laying and operation of the suspended cables by defining three phases (i.e., stressing, lifting, and operation). The phenomena that occurred in each phase were analyzed, as was their manifestation in the cable model. Herein, we developed and validated the solution of the steady-static response equation in suspended cables using simple equations supported with intuitive graphics. The best results of the proposed calculation procedure were obtained in conditions of large temperature variations.