bluuebunny/crossref_metadata_2025 huggingface dataset:
Time Series Surface Temperature Prediction Based on Cyclic Evolutionary Network Model for Complex Sea Area
Authors: Shi Jiahao , Yu Jie , Yang Jinkun , Xu Lingyu , Xu Huan
Published: March 2022

The prediction of marine elements has become increasingly important in the field of marine research. However, time series data in a complex environment vary significantly because they are composed of dynamic changes with multiple mechanisms, causes, and laws. For example, sea surface temperature (SST) can be influenced by ocean currents. Conventional models often focus on capturing the impact of historical data but ignore the spatio–temporal relationships in sea areas, and they cannot predict such widely varying data effectively. In this work, we propose a cyclic evolutionary network model (CENS), an error-driven network group, which is composed of multiple network node units. Different regions of data can be automatically matched to a suitable network node unit for prediction so that the model can cluster the data based on their characteristics and, therefore, be more practical. Experiments were performed on the Bohai Sea and the South China Sea. Firstly, we performed an ablation experiment to verify the effectiveness of the framework of the model. Secondly, we tested the model to predict sea surface temperature, and the results verified the accuracy of CENS. Lastly, there was a meaningful finding that the clustering results of the model in the South China Sea matched the actual characteristics of the continental shelf of the South China Sea, and the cluster had spatial continuity.
Large-Scale Enclosure Fire Experiments Adopting CLT Slabs with Different Types of Polyurethane Adhesives: Genesis and Preliminary Findings
Authors: Hopkin Danny , Węgrzyński Wojciech , Spearpoint Michael , Fu Ian , Krenn Harald , Sleik Tim , Gorska Carmen , Stapf Gordian
Published: March 2022

This paper provides understanding of the fire performance of exposed cross-laminated-timber (CLT) in large enclosures. An office-type configuration has been represented by a 3.75 by 7.6 by 2.4 m high enclosure constructed of non-combustible blockwork walls, with a large opening on one long face. Three experiments are described in which propane-fuelled burners created a line fire that impinged on different ceiling types. The first experiment had a non-combustible ceiling lining in which the burners were set to provide flames that extended approximately halfway along the underside of the ceiling. Two further experiments used exposed 160 mm thick (40-20-40-20-40 mm) loaded CLT panels with a standard polyurethane adhesive between lamella in one experiment and a modified polyurethane adhesive in the other. Measurements included radiative heat flux to the ceiling and the floor, temperatures within the depth of the CLT and the mass loss of the panels. Results show the initial peak rate of heat release with the exposed CLT was up to three times greater when compared with the non-combustible lining. As char formed, this stabilised at approximately one and a half times that of the non-combustible lining. Premature char fall-off (due to bond-line failure) was observed close to the burners in the CLT using standard polyurethane adhesive. However, both exposed CLT ceiling experiments underwent auto-extinction of flaming combustion once the burners were switched off.
Simplified CFD Model for Perforated Tile with Distorted Outflow
Authors: Abdelmaksoud Waleed A.
Published: March 2022

Most of the perforated tile flow CFD models in the literature so far assume the air velocity coming out of the tile openings (pores) is uniform. However, in typical applications, such as data centers and indoor environments, perforated tile or diffuser outflow can be highly non-uniform due to many reasons (e.g., spatial variation of plenum pressure or varying local tile geometrical patterns). For an ideal (uniform) tile flow velocity that has the same flow rate as the non-uniform tile flow velocity, the tile flow momentum of the latter will always be greater because momentum scales with velocity squared. To illustrate the effect of tile flow velocity distortion, two generic CFD cases (one with uniform velocity and the other with non-uniform velocity) with multiple openings model are presented here. Their CFD results are compared to the momentum source model results and validated against previously published data of an isolated tile flow measurement. The momentum source model is one of the simplest/most practical CFD models that uses a body-force value for correcting the momentum deficit between perforated and fully open areas. Initially, the momentum source model results show good agreement with results of the uniform velocity case only. Thus, due to velocity distortion (non-uniform velocity) in the tile flow, the CFD results presented in this paper show a potential reason to modify the body-force value in the momentum source model with an adjustment factor (C). Several values of C factor are numerically investigated for the present distorted tile flow CFD case, and the best match is found to be at C, equaling approximately 1.6.
Current Trends in Fluid Research in the Era of Artificial Intelligence: A Review
Authors: Sofos Filippos , Stavrogiannis Christos , Exarchou-Kouveli Kalliopi K. , Akabua Daniel , Charilas George , Karakasidis Theodoros E.
Published: March 2022

Computational methods in fluid research have been progressing during the past few years, driven by the incorporation of massive amounts of data, either in textual or graphical form, generated from multi-scale simulations, laboratory experiments, and real data from the field. Artificial Intelligence (AI) and its adjacent field, Machine Learning (ML), are about to reach standardization in most fields of computational science and engineering, as they provide multiple ways for extracting information from data that turn into knowledge, with the aid of portable software implementations that are easy to adopt. There is ample information on the historical and mathematical background of all aspects of AI/ML in the literature. Thus, this review article focuses mainly on their impact on fluid research at present, highlighting advances and opportunities, recognizing techniques and methods having been proposed, tabulating, and testing some of the most popular algorithms that have shown significant accuracy and performance on fluid applications. We also investigate algorithmic accuracy on several fluid datasets that correspond to simulation results for the transport properties of fluids and suggest that non-linear, decision tree-based methods have shown remarkable performance on reproducing fluid properties.
Influence of Turbulence Effects on the Runup of Tsunami Waves on the Shore within the Framework of the Navier–Stokes Equations
Authors: Kozelkov Andrey , Tyatyushkina Elena , Kurulin Vadim , Kurkin Andrey
Published: March 2022

This paper considers turbulence effects on tsunami runup on the shore in tsunami simulations using the system of three-dimensional Navier–Stokes equations. The turbulence effects in tsunami propagation and runup are studied by solving the problem of a wave propagating in a nonuniform-bottom pool and collapsing with a barrier. To solve this problem, we used the turbulence model, RANS SST (Reynolds-averaged Navier–Stokes shear stress transport). We compared the wave profiles at different times during wave propagation, runup, and collapse. To quantify the turbulence effects, we also compared the forces acting on the basin bottom. We demonstrated that the turbulence had almost no effect on the shape of the wave and the way of its propagation (except collapse). However, turbulence effects during the runup and collapse became noticeable and could boost the flow (increasing the pressure force and the total force) by up to 25 percent.
The Solubility and Structures of Porcine Myofibrillar Proteins under Low-Salt Processing Conditions as Affected by the Presence of L-Lysine
Authors: Li Xiuping , Wang Wenhui , Wang Shouyin , Shen Yuqing , Pan Jinfeng , Dong Xiuping , Li Shengjie
Published: March 2022

This study aimed to investigate the presence of L-lysine (Lys) on the solubility and structures of myofibrillar proteins (MFPs) at different ionic strengths. Porcine MFPs were incubated at 4 °C with various levels of ionic strengths (0.15, 0.3, or 0.6 M NaCl) with or without the presence of 20 or 40 mM Lys. After 24 h of incubation, MFP solubility and turbidity were determined, and the particle size distribution, circular dichroism spectra, and intrinsic tryptophan fluorescence of MFP were analyzed to obtain their secondary and tertiary structure. Results showed that the solubilization effects of Lys on MFPs are dependent on the ionic strength. Particularly, the presence of Lys could improve MFP solubility at 0.3 M, which resembles salt-reducing processing conditions. Concomitantly, the secondary and tertiary structures were observed to change as a result of the varying ionic strengths and the addition of Lys, including myofibril swelling, dissociation of myosin filaments, uncoiling of α-helix, and unfolding of the tertiary structure. The possible mechanisms underlying the solubilization effects of Lys on MFPs at low ionic strengths are discussed from the perspective of protein structural changes.
Using RSM for Optimum of Optimum Production of Peptides from Edible Bird’s Nest By-Product and Characterization of Its Antioxidant’s Properties
Authors: Cao Jie , Xiong Ning , Zhang Yu , Dai Yuwei , Wang Yuye , Lu Lingyu , Jiang Lin
Published: March 2022

In this research, the neutrase hydrolysis conditions of edible bird’s nest (EBN) by-products were optimized by response surface methodology (RSM). Antioxidant peptides were then isolated from the EBN by-products by ultrafiltration and chromatography taking the DPPH radical scavenging ability as an indicator. The antioxidant activity of the purified peptides was estimated by radical scavenging ability and sodium nitroprusside (SNP)-induced damage model in PC12 cells. When the enzyme concentration was10 kU/g-hydrolysis temperature was 45 °C, and hydrolysis time was 10.30 h, the degree of hydrolysis (DH) of EBN by-product hydrolysate (EBNH) was the highest. The purified peptide exerted strong scavenging ability with EC50 values of 0.51, 1.31, and 0.65 mg/mL for DDPH, ABTS, and O2− radicals, respectively. In addition, the purified peptides could significantly reduce the SNP-induced oxidative damage of PC12 cells, and twelve peptides that were rich in leucine (Leu), valine (Val), and lysine (Lys) were identified by LC-MS/MS. These results suggested that EBN by-products have potential as new materials for natural antioxidant peptides.
Ceviche-Natural Preservative: Possibility of Microbiota Survival and Effect on L. monocytogenes
Authors: Zakrzewski Arkadiusz Józef , Chajęcka-Wierzchowska Wioleta , Zadernowska Anna
Published: March 2022

Ceviche is a marinated raw fish dish ready for consumption; it is a part of the cuisine of various countries on the Pacific coast and its preparation may differ among them. Although the process uses the traditional method of food preservation by lowering the pH, the exposure time is very limited, so the aim of the study was to determine the viability of bacteria often isolated from fish after the process of preparing traditional ceviche. For this purpose, the traditional plate method and flow cytometry were used, and for pathogenic L. monocytogenes strains, the influence of stress during the preparation of the dish on the pathogenic potential was determined. The study showed that the highest percentage of viable cells was observed in the case of L. monocytogenes and remained at the level of 98.54%, slightly less for L. innocua, 96.93%. For the remaining species the reduction did not exceed 10%, for E. faecalis it was 92.76%, for S. liqefaciens 91.44%, H. alvei 93.68%. In addition, the study of the antibacterial properties of individual ingredients showed that habanero and coriander did not show any bactericidal effect, while for onions the amount of live cells was 99.11%, and for lime juice 97.26%, Additionally, the study of changes in virulence, antibiotic resistance and gene expression showed that the stress during the preparation of ceviche has different effects depending on the strain and may cause virulence potential increase, levofloxacin and daptomycin minimum inhibiotory concentration increase and some crucial virulence gene expression induction; therefore, it is important to take care of the quality of the products used to prepare the ceviche and accurate pretreatment.
Plant Extract and Essential Oil Application against Food-Borne Pathogens in Raw Pork Meat
Authors: Mantzourani Ioanna , Daoutidou Maria , Dasenaki Marilena , Nikolaou Anastasios , Alexopoulos Athanasios , Terpou Antonia , Thomaidis Nikolaos , Plessas Stavros
Published: March 2022

Herbal and plant extracts are being applied for a wide range of foods against different types of food-borne pathogens. In the present study, ethanolic and aqueous extracts (2% w/v) from cranberry (Vaccinium macrocarpon) and pomegranate (Punica granatum L.) plants were applied alone or in combination with two essential oils (thyme and oregano in a concentration of 0.150 μg/g) in pork meatballs and their antimicrobial activity was estimated. The extracts exhibited promising results (aqueous and ethanolic extracts of pomegranate and cranberry in a food-compatible concentration of 2% w/v) were applied to raw pork meatball production and their antimicrobial activity was recorded versus Enterobacteriaceae, total mesophilic bacteria, yeasts/molds, Staphylococcus spp., Pseudomonas spp. and lactic acid bacteria (LAB). The outcome demonstrated that meatballs containing aqueous extracts of pomegranate were more resistant to spoilage compared to all the other samples since they were preserved for more days. The chemical profiles of plant extracts were determined through LC-QTOF/MS and the chemical composition of the essential oils applied was determined with the use of GC/MS in order to identify the substances involved in the observed antimicrobial activity. Phenolic acids (quinic acid, chlorogenic acid), monoterpenes (p-cymene, carvacrol, thymol, limonene), organic acids (citric acid) and phenols were the main constituents found in the plant extracts and essential oils applied. These extracts of plant origin could be used as natural preservatives in meat products, even in low concentrations.
Microbiological and Enzymatic Activity Modulates the Bitter Taste Reduction in Decanted Coratina Olive Oil
Authors: Ciafardini Gino , Zullo Biagi Angelo
Published: March 2022

Coratina monocultivar extra virgin olive oil (EVOO) is known for its level of bitterness, which, if too high, can cause consumer acceptance problems. The aim of this study was to modulate the bitter taste of freshly produced olive oil through endogenous enzymatic activity and microbiota during the decantation phase. The opalescent appearance of the newly produced EVOO was substantially reduced during the first three months of decantation due to the deposition of more than 90% of suspended material, consisting of vegetation water and suspended solid particles. The high content of biophenols and the reduction in water concentration in the oil samples negatively affected the survival of yeasts, which were absent in the oil samples at the end of the third month of decantation. The oleuropeinolytic activity was very intense during the first month of decantation, whereas the reduction in the bitter taste associated with the aglycons was consistent only in the second and third months of decantation. At the end of decantation, the sensory notes of bitterness in the Coratina EVOO were reduced by 33%, lowering the position on the value scale without altering the other qualitative parameters whose values fell within the limits of the commercial EVOO class.
Evaluation of Modified Atmosphere Packaging in Combination with Active Packaging to Increase Shelf Life of High-in Beta-Glucan Gluten Free Cake
Authors: Wyrwisz Jarosław , Karp Sabina , Kurek Marcin Andrzej , Moczkowska-Wyrwisz Małgorzata
Published: March 2022

Modified atmosphere packaging and active packaging were combined to prolong the shelf life and quality of the clean label, gluten-free (GF), yeast-leavened cakes enriched in oat fiber preparation. Star anise, cinnamon bark, and clove essential oils were used as emitters of active substances. The following concentrations of gases were chosen: 0% CO2/100%/N2 (MAP1), 60% CO2/40% N2 (MAP2), and approx. 78% N2/21% O2/0.04% CO2 (ATM). Microbiological and physicochemical analyses were conducted. GF cakes were stored for 14 days (analysis in 0, 7, and 14 days). The results showed a decrease in moisture content and lightness of crumb and an increase in hardness. EOs significantly (p ≤ 0.05) slowed down the growth of microorganisms regardless of the type of gas mixture. However, the best bacteriostatic effect was in MAP2. The content of beta-glucan did not change throughout the storage time. Generally, the best results were obtained with the combination of MAP and active packaging—60% of CO2 and 40% of N2—where cinnamon or clove essential oils were used.
Ingredients, Processing, and Fermentation: Addressing the Organoleptic Boundaries of Plant-Based Dairy Analogues
Authors: Pua Aileen , Tang Vivien Chia Yen , Goh Rui Min Vivian , Sun Jingcan , Lassabliere Benjamin , Liu Shao Quan
Published: March 2022

Consumer interest and research in plant-based dairy analogues has been growing in recent years because of increasingly negative implications of animal-derived products on human health, animal wellbeing, and the environment. However, plant-based dairy analogues face many challenges in mimicking the organoleptic properties of dairy products due to their undesirable off-flavours and textures. This article thus reviews fermentation as a viable pathway to developing clean-label plant-based dairy analogues with satisfactory consumer acceptability. Discussions on complementary strategies such as raw material selection and extraction technologies are also included. An overview of plant raw materials with the potential to be applied in dairy analogues is first discussed, followed by a review of the processing steps and innovative techniques required to transform these plant raw materials into functional ingredients such as plant-based aqueous extracts or flours for subsequent fermentation. Finally, the various fermentation (bacterial, yeast, and fungal) methodologies applied for the improvement of texture and other sensory qualities of plant-based dairy analogues are covered. Concerted research efforts would be required in the future to tailor and optimise the presented wide diversity of options to produce plant-based fermented dairy analogues that are both delicious and nutritionally adequate.
Comparison of Flavonoid O-Glycoside, C-Glycoside and Their Aglycones on Antioxidant Capacity and Metabolism during In Vitro Digestion and In Vivo
Authors: Xie Liangqin , Deng Zeyuan , Zhang Jie , Dong Huanhuan , Wang Wei , Xing Banghuai , Liu Xiaoru
Published: March 2022

Flavonoids are well known for their extensive health benefits. However, few studies compared the differences between flavonoid O-glycoside and C-glycoside. In this work, flavonoid O-glycoside (isoquercitrin), C-glycoside (orientin), and their aglycones (quercetin and luteolin) were chosen to compare their differences on antioxidant activities and metabolism during in vitro digestion and in vivo. In vitro digestion, the initial antioxidant activity of the two aglycones was very high; however, they both decreased more sharply than their glycosides in the intestinal phase. The glycosidic bond of flavonoid O-glycoside was broken in the gastric and intestinal stage, while the C-glycoside remained unchanged. In vivo, flavonoid O-glycoside in plasma was more elevated than C-glycoside on the antioxidant activity; however, flavonoid C-glycoside in urine was higher than O-glycoside. These results indicate that differences of flavonoid glycosides and their aglycones on antioxidant activity are closely related to their structural characteristics and metabolism in different samples. Aglycones possessed higher activity but unstable structures. On the contrary, the sugar substituents reduced the activity of flavonoids while improving their stability and helping to maintain antioxidant activities after digestion. Especially the C-glycoside was more stable because the stability of the C–C bond is higher than that of the C–O bond, which contributes to the difference between flavonoid O-glycoside and C-glycoside on the absorption and metabolism in vivo. This study provided a new perspective for comparing flavonoid O-glycoside, flavonoid C-glycoside, and their aglycones on their structure–activity relationship and metabolism.
The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis
Authors: Li Shanshan , Huo Xiaohui , Qi Yuli , Ren Duoduo , Li Zhiman , Qu Di , Sun Yinshi
Published: March 2022

Polysaccharides from Panax ginseng are natural carbohydrates with multiple activities. However, little was known about its functions on colitis. In this study, we aim to investigate the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis. Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography. An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively. Both WGP and WGPA alleviated the colitis symptoms and colon structure changes of colitis rats. They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs. In conclusion, WGP exerted a protective effect against colitis with its acidic fraction (WGPA) as an effective fraction. The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics
Authors: Xie Jing , Huang Jianhua , Ren Guangxi , Jin Jian , Chen Lin , Zhong Can , Cai Yuan , Liu Hao , Zhou Rongrong , Qin Yuhui , Zhang Shuihan
Published: March 2022

Poria cocos (PC) is an important fungus with high medicinal and nutritional values. However, the quality of PC is heavily dependent on multiple factors in the cultivation regions. Traditional methods are not able to perform quality evaluation for this fungus in a short time, and a new method is needed for rapid quality assessment. Here, we used near-infrared (NIR) spectroscopy combined with chemometric method to identify the cultivation regions and determine PC chemical compositions. In our study, 138 batches of samples were collected and their cultivation regions were distinguished by combining NIR spectroscopy and random forest method (RFM) with an accuracy as high as 92.59%. In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT). The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively. Overall, a new approach was developed and validated which is able to distinguish PC production regions, quantify its chemical contents, and effectively evaluate PC quality.
New Explicit Solutions of the Extended Double (2+1)-Dimensional Sine-Gorden Equation and Its Time Fractional Form
Authors: Wang Gangwei , Li Li , Wang Qi , Geng Juan
Published: March 2022

In this paper, the extended double (2+1)-dimensional sine-Gorden equation is studied. First of all, using the symmetry method, the corresponding vector fields, Lie algebra and infinitesimal generators are derived. Then, from infinitesimal generators, the symmetry reductions are presented. In addition, these reduced equations are converted into the corresponding partial differential equations, which including classical double (1+1)-dimensional sine-Gorden equation. Moreover, based on the Lie symmetry method again, these reduced equations are investigated. Meanwhile, based on traveling wave transformation, some explicit solutions of the extended double (2+1)-dimensional sine-Gorden equation are obtained. Consequently, a conservation law is derived via conservation law multiplier method. Finally, especially with the help of the fractional complex transform, some solutions of double time fractional (2+1)-dimensional sine-Gorden equation are also derived. These results might explain complex nonlinear phenomenon.
A Local Fractional Elzaki Transform Decomposition Method for the Nonlinear System of Local Fractional Partial Differential Equations
Authors: Anac Halil
Published: March 2022

In this paper, the nonlinear system of local fractional partial differential equations is solved via local fractional Elzaki transform decomposition method. The local fractional Elzaki decomposition transform method combines a local fractional Elzaki transform and the Adomian decomposition method. Applications related to the nonlinear system of local fractional partial differential equations are presented.
Inequalities for Fractional Integrals of a Generalized Class of Strongly Convex Functions
Authors: Yan Tao , Farid Ghulam , Yasmeen Hafsa , Shim Soo Hak , Jung Chahn Yong
Published: March 2022

Fractional integral operators are useful tools for generalizing classical integral inequalities. Convex functions play very important role in the theory of mathematical inequalities. This paper aims to investigate the Hadamard type inequalities for a generalized class of functions namely strongly (α,h−m)-p-convex functions by using Riemann–Liouville fractional integrals. The results established in this paper give refinements of various well-known inequalities which have been published in the recent past.
New Fractional Integral Inequalities for Convex Functions Pertaining to Caputo–Fabrizio Operator
Authors: Sahoo Soubhagya Kumar , Mohammed Pshtiwan Othman , Kodamasingh Bibhakar , Tariq Muhammad , Hamed Y. S.
Published: March 2022

In this article, a generalized midpoint-type Hermite–Hadamard inequality and Pachpatte-type inequality via a new fractional integral operator associated with the Caputo–Fabrizio derivative are presented. Furthermore, a new fractional identity for differentiable convex functions of first order is proved. Then, taking this identity into account as an auxiliary result and with the assistance of Hölder, power-mean, Young, and Jensen inequality, some new estimations of the Hermite-Hadamard (H-H) type inequality as refinements are presented. Applications to special means and trapezoidal quadrature formula are presented to verify the accuracy of the results. Finally, a brief conclusion and future scopes are discussed.
On Geometric Properties of a Certain Analytic Function with Negative Coefficients
Authors: Oluwayemi Matthew Olanrewaju , Davids Esther O. , Cătaş Adriana
Published: March 2022

Various function theorists have successfully defined and investigated different kinds of analytic functions. The applications of such functions have played significant roles in geometry function theory as a field of complex analysis. In this work, therefore, a certain subclass of univalent analytic functions of the form f(z)=z−∑m=2t[ω(2+β)+cγ−σ]Cm[mσ−cω(2+β)+cγ]Knzm−∑k=t+1∞akzk is defined using a generalized differential operator. Furthermore, some geometric properties for the class were established.
The Dynamical Analysis of a Biparametric Family of Six-Order Ostrowski-Type Method under the Möbius Conjugacy Map
Authors: Wang Xiaofeng , Chen Xiaohe
Published: March 2022

In this paper, a family of Ostrowski-type iterative schemes with a biparameter was analyzed. We present the dynamic view of the proposed method and study various conjugation properties. The stability of the strange fixed points for special parameter values is studied. The parameter spaces related to the critical points and dynamic planes are used to visualize their dynamic properties. Eventually, we find the most stable member of the biparametric family of six-order Ostrowski-type methods. Some test equations are examined for supporting the theoretical results.
Investigating the Immunomodulatory Potential of Dental Pulp Stem Cell Cultured on Decellularized Bladder Hydrogel towards Macrophage Response In Vitro
Authors: Nguyen Huynh-Quang-Dieu , Kao Chen-Yu , Chiang Chien-Ping , Hung Yu-Han , Lo Chun-Min
Published: March 2022

Mesenchymal stem cells (MSCs) possess immunomodulatory properties and capacity for endogenous regeneration. Therefore, MSC therapy is a promising treatment strategy for COVID-19. However, the cells cannot stay in the lung long enough to exert their function. The extracellular matrix from porcine bladders (B-ECM) has been shown not only to regulate cellular activities but also to possess immunoregulatory characteristics. Therefore, it can be hypothesized that B-ECM hydrogel could be an excellent scaffold for MSCs to grow and could anchor MSCs long enough in the lung so that they can exhibit their immunomodulatory functions. In this study, ECM degradation products and a co-culture system of MSCs and macrophages were developed to study the immunomodulatory properties of ECM and MSCs under septic conditions. The results showed that B-ECM degradation products could decrease pro-inflammatory and increase anti-inflammatory cytokines from macrophages. In an in vivo mimicking co-culture system, MSCs cultured on B-ECM hydrogel exhibited immunomodulatory properties at both gene and protein levels. Both B-ECM degradation products and MSC conditioned medium supported the wound healing of alveolar epithelial cells. The results from the study could offer a basis for investigation of immunomodulation by ECM and MSCs before conducting in vivo experiments, which could later be applied in regenerative medicine.
4,6-O-Phenylethylidene Acetal Protected D-Glucosamine Carbamate-Based Gelators and Their Applications for Multi-Component Gels
Authors: Sharma Pooja , Wang Guijun
Published: March 2022

The self-assembly of carbohydrate-based low molecular weight gelators has led to useful advanced soft materials. The interactions of the gelators with various cations and anions are important in creating novel molecular architectures and expanding the scope of the small molecular gelators. In this study, a series of thirteen new C-2 carbamates of the 4,6-O-phenylethylidene acetal-protected D-glucosamine derivatives has been synthesized and characterized. These compounds are rationally designed from a common sugar template. All carbamates synthesized were found to be efficient gelators and three compounds are also hydrogelators. The resulting gels were characterized using optical microscopy, atomic force microscopy, and rheology. The gelation mechanisms were further elucidated using 1H NMR spectroscopy at different temperatures. The isopropyl carbamate hydrogelator 7 formed hydrogels at 0.2 wt% and also formed gels with several tetra alkyl ammonium salts, and showed effectiveness in the creation of gel electrolytes. The formation of metallogels using earth-abundant metal ions such as copper, nickel, iron, zinc, as well as silver and lead salts was evaluated for a few gelators. Using chemiluminescence spectroscopy, the metal–organic xerogels showed enzyme-like properties and enhanced luminescence for luminol. In addition, we also studied the applications of several gels for drug immobilizations and the gels showed sustained release of naproxen from the gel matrices. This robust sugar carbamate-derived gelator system can be used as the scaffold for the design of other functional materials with various types of applications.
Highly Responsive Chitosan-Co-Poly (MAA) Nanomatrices through Cross-Linking Polymerization for Solubility Improvement
Authors: Saleem Anam , Akhtar Naveed , Minhas Muhammad Usman , Mahmood Arshad , Khan Kifayat Ullah , Abdullah Orva
Published: March 2022

In this study, we report the highly responsive chitosan-based chemically cross-linked nanomatrices, a nano-version of hydrogels developed through modified polymerization reaction for solubility improvement of poorly soluble drug simvastatin. The developed nanomatrices were characterized for solubilization efficiency, swelling studies, sol-gel analysis, in vitro drug release studies, DSC, FTIR, XRD, SEM, particle size analysis, and stability studies. An in vivo acute toxicity study was conducted on female Winstor rats, the result of which endorsed the safety and biocompatibility of the system. A porous and fluffy structure was observed under SEM analysis, which supports the great swelling tendency of the system that further governs the in vitro drug release. Zeta sizer analyzed the particle size in the range of 227.8 ± 17.8 nm. Nano sizing and grafting of hydrophilic excipients to the nanomatrices system explains this shift of trend towards the enhancement of solubilization efficiency, and, furthermore, the XRD results confirmed the amorphous nature of the system. FTIR and DSC analysis confirmed the successful grafting and stability to the system. The developed nanomatrices enhanced the release characteristics and solubility of simvastatin significantly and could be an effective technique for solubility and bioavailability enhancement of other BCS class-II drugs. Due to enhanced solubility, efficient method of preparation, excellent physico-chemical features, and rapid and high dissolution and bio-compatibility, the developed nanomatrices may be a promising approach for oral delivery of hydrophobic drugs.
Identification of Genetic Risk Factors of Severe COVID-19 Using Extensive Phenotypic Data: A Proof-of-Concept Study in a Cohort of Russian Patients
Authors: Shcherbak Sergey G. , Changalidi Anton I. , Barbitoff Yury A. , Anisenkova Anna Yu. , Mosenko Sergei V. , Asaulenko Zakhar P. , Tsay Victoria V. , Polev Dmitrii E. , Kalinin Roman S. , Eismont Yuri A. , Glotov Andrey S. , Garbuzov Evgeny Y. , Chernov Alexander N. , Klitsenko Olga A. , Ushakov Mikhail O. , Shikov Anton E. , Urazov Stanislav P. , Baranov Vladislav S. , Glotov Oleg S.
Published: March 2022

The COVID-19 pandemic has drawn the attention of many researchers to the interaction between pathogen and host genomes. Over the last two years, numerous studies have been conducted to identify the genetic risk factors that predict COVID-19 severity and outcome. However, such an analysis might be complicated in cohorts of limited size and/or in case of limited breadth of genome coverage. In this work, we tried to circumvent these challenges by searching for candidate genes and genetic variants associated with a variety of quantitative and binary traits in a cohort of 840 COVID-19 patients from Russia. While we found no gene- or pathway-level associations with the disease severity and outcome, we discovered eleven independent candidate loci associated with quantitative traits in COVID-19 patients. Out of these, the most significant associations correspond to rs1651553 in MYH14p = 1.4 × 10−7), rs11243705 in SETX (p = 8.2 × 10−6), and rs16885 in ATXN1 (p = 1.3 × 10−5). One of the identified variants, rs33985936 in SCN11A, was successfully replicated in an independent study, and three of the variants were found to be associated with blood-related quantitative traits according to the UK Biobank data (rs33985936 in SCN11A, rs16885 in ATXN1, and rs4747194 in CDH23). Moreover, we show that a risk score based on these variants can predict the severity and outcome of hospitalization in our cohort of patients. Given these findings, we believe that our work may serve as proof-of-concept study demonstrating the utility of quantitative traits and extensive phenotyping for identification of genetic risk factors of severe COVID-19.
Rif1-Dependent Control of Replication Timing
Authors: Richards Logan , Das Souradip , Nordman Jared T.
Published: March 2022

Successful duplication of the genome requires the accurate replication of billions of base pairs of DNA within a relatively short time frame. Failure to accurately replicate the genome results in genomic instability and a host of diseases. To faithfully and rapidly replicate the genome, DNA replication must be tightly regulated and coordinated with many other nuclear processes. These regulations, however, must also be flexible as replication kinetics can change through development and differentiation. Exactly how DNA replication is regulated and how this regulation changes through development is an active field of research. One aspect of genome duplication where much remains to be discovered is replication timing (RT), which dictates when each segment of the genome is replicated during S phase. All organisms display some level of RT, yet the precise mechanisms that govern RT remain are not fully understood. The study of Rif1, a protein that actively regulates RT from yeast to humans, provides a key to unlock the underlying molecular mechanisms controlling RT. The paradigm for Rif1 function is to delay helicase activation within certain regions of the genome, causing these regions to replicate late in S phase. Many questions, however, remain about the intricacies of Rif1 function. Here, we review the current models for the activity of Rif1 with the goal of trying to understand how Rif1 functions to establish the RT program.
In Vitro Analysis of Biological Activity of Circulating Cell-Free DNA Isolated from Blood Plasma of Schizophrenic Patients and Healthy Controls
Authors: Ershova Elizaveta S. , Shmarina Galina V. , Porokhovnik Lev N. , Zakharova Natalia V. , Kostyuk George P. , Umriukhin Pavel E. , Kutsev Sergey I. , Sergeeva Vasilina A. , Veiko Natalia N. , Kostyuk Svetlana V.
Published: March 2022

Schizophrenia is associated with low-grade systemic inflammation. Circulating cell-free DNA (c-cfDNA) belongs to the DAMP class. The major research question was: can the c-cfDNA of schizophrenic patients (sz-cfDNA) stimulate the DNA sensor genes, which control the innate immunity? We investigated the in vitro response of ten human skin fibroblast (HSF) lines to five DNA probes containing different amounts of a GC-rich marker (the ribosomal repeat) and a DNA oxidation marker (8-oxodG) including sz-cfDNA and healthy control c-cfDNA (hc-cfDNA) probes. After 1 h, 3 h, and 24 h of incubation, the expression of 6 protein genes responsible for cfDNA transport into the cell (EEA1 and HMGB1) and the recognition of cytosolic DNA (TLR9, AIM2, STING and RIG-I) was analyzed at the transcriptional (RT-qPCR) and protein level (flow cytometry and fluorescence microscopy). Additionally, we analyzed changes in the RNA amount of 32 genes (RT-qPCR), which had been previously associated with different cellular responses to cell-free DNA with different characteristics. Adding sz-cfDNA and hc-cfDNA to the HSF medium in equal amounts (50 ng/mL) blocked endocytosis and stimulated TLR9 and STING gene expression while blocking RIG-I and AIM2 expression. Sz-cfDNA and hc-cfDNA, compared to gDNA, demonstrated much stronger stimulated transcription of genes that control cell proliferation, cytokine synthesis, apoptosis, autophagy, and mitochondrial biogenesis. No significant difference was observed in the response of the cells to sz-cfDNA and hc-cfDNA. Sz-cfDNA and hc-cfDNA showed similarly high biological activity towards HSFs, stimulating the gene activity of TLR9 and STING DNA sensor proteins and blocking the activity of the AIM2 protein gene. Since the sz-cfDNA content in the patients’ blood is several times higher than the hc-cfDNA content, sz-cfDNA may upregulate pro-inflammatory cytokines in schizophrenia.
Coronavirus Genomes and Unique Mutations in Structural and Non-Structural Proteins in Pakistani SARS-CoV-2 Delta Variants during the Fourth Wave of the Pandemic
Authors: Anwar Muhammad Zeeshan , Lodhi Madeeha Shahzad , Khan Muhammad Tahir , Khan Malik Ihsanullah , Sharif Sumaira
Published: March 2022

Genomic epidemiology of SARS-CoV-2 is imperative to explore the transmission, evolution, and also pathogenicity of viruses. The emergence of SARS-CoV-2 variants of concern posed a severe threat to the global public health efforts. To assess the potential consequence of these emerging variants on public health, continuous molecular epidemiology is of vital importance. The current study has been designed to investigate the major SARS-CoV-2 variants and emerging mutations in virus structural and non-structural proteins (NSP) during the fourth wave in September 2021 from the Punjab province of Pakistan. Twenty SARS-CoV-2 positive samples have been collected from major cities were subjected to next-generation sequencing. Among the 20 whole genomes (GenBank Accession SRR16294858-SRR16294877), 2 samples failed to be completely sequenced. These genome sequences harbored 207 non-synonymous mutations, among which 19 were unique to GISAID. The genome sequences were detected: Delta 21I, 21J variants (B.1.617.2). Mutation’s spike_F157del, spike_P681R, spike_T478K, spike_T19R, spike_L452R, spike_D614G, spike_G142D, spike_E156G, and spike_R158del have been detected in all samples where K1086Q, E554K, and C1250W were unique in spike protein. These genomic sequences also harbored 129 non-synonymous mutations in NSP. The most common were NSP3_P1469S (N = 17), NSP3_A488S (N = 17), NSP3_P1228L (N = 17), NSP4_V167L (N = 17), NSP4_T492I (N = 17), NSP6_T77A (N = 17), NSP14_A394V (N = 17), NSP12_G671S (N = 18), and NSP13_P77L (N = 18). The mutation, F313Y in NSP12, detected in the current study, was found in a single isolate from Belgium. Numerous other unique mutations have been detected in the virus papain-like protease (NSP3), main protease (NSP5), and RNA-dependent RNA polymerase (NSP12). The most common non-synonymous mutations in the spike protein were subjected to stability analysis, exhibiting a stabilizing effect on structures. The presence of Delta variants may affect therapeutic efforts and vaccine efficacy. Continuous genomic epidemiology of SARS-CoV-2 in Pakistan may be useful for better management of SARS-CoV-2 infections.
NO FUTURE: The Colonial Gaze, Tales of Return in Recent Latin American Film
Authors: Rojas-Sotelo Miguel L.
Published: March 2022

The past is certain, the future an illusion. Contemporary films such as Ivy Maraey: land without evil (Juan Carlos Valdivia 2013), Embrace of the Serpent (Ciro Guerra 2015), The Fever (Maya Da Rin 2020), and Bacurau are border films, from the genre of contact films. They announce how coloniality maintains a grip on frontier territories in the Americas. These films also present particular indigenous visions that challenge western epistemes and confront audiences with particular ways of being in the world, where the modern subject finds its limit. The article introduces a critical perspective on cinema as a colonial tool, producing forms of capture that are part of the modern archive and the notion of linear time. These films also build on cinematic traditions such as tercer cine and afro-futurism, and are strong on concepts such as cosmopolitanism, resistance, and subalternity. They present forms of adaptation, reaction, return, and redemption while maintaining the status of cinema as a capturing device, entertainment, and capital investment (the triad of destruction in modernity/coloniality).
A Content Analysis of Osteopaths’ Attitudes for a More Inclusive Clinical Practice towards Transgender People
Authors: Baldin Irene , Esteves Jorge E. , Tramontano Marco , Macdonald Mia , Baroni Francesca , Lunghi Christian
Published: March 2022

Objectives. The aim of this qualitative study was to explore the attitudes, beliefs, and preferences of Italian osteopaths regarding the management of transgender patients through a content analysis of emergent data from semi-structured interviews. Methods. This study was a content analysis based on the Standards for Reporting Qualitative Research guidelines. Purposive sampling of 10 Italian osteopaths was applied. Data were collected through semi-structured interviews, from March to April 2021, and subsequently transcribed verbatim with the content analysis carried out as an iterative process. Results. One participant was excluded during the first interview due to them being unsuitable for this study. Data saturation was reached after two interviews with the remaining nine participants. Data analysis revealed four main themes: microaggressions, acceptance and non-judgement, person-centered treatment, and education implementation. Conclusions. This study presents cisgender Italian osteopaths’ attitudes in the care of transgender people, revealing the desire to embrace and apply osteopathic tenets regardless of the patient’s gender identity.
3D Low-Cost Equipment for Automated Peritoneal Dialysis Therapy
Authors: Rivero-Urzua Samuel , Paredes-Rojas Juan Carlos , Méndez-García Sergio Rodrigo , Ortiz-Hernández Fernando Eli , Oropeza-Osornio Armando , Torres-SanMiguel Christopher René
Published: March 2022

A breakthrough in peritoneal dialysis (PD) therapy occurred in 1977 with the development of continuous ambulatory peritoneal dialysis (CAPD). Its simplicity, low cost, and ease with which CAPD could be performed on patients at home contributed to the popularity of this procedure. However, there is a need for continuous improvement in building optimal systems for incident chronic kidney disease (CKD) patients. This research showed the design and construction of a simplified prototype of low-cost automated peritoneal dialysis (APD) equipment that meets international standards to automatically regulate infusion and fluid drainage in and out of a patient with low margins of error. Experimental tests allowed the adjustment of the RPM values concerning the flow rate provided. In addition, thanks to the pressure sensor, it was possible to observe a fluctuation ranging from 9 to 13 kPa, which is within the permissible average specified in the catalogs of medical instruments and equipment. Furthermore, a turbidity sensor was added to decrease the possibility of presenting peritonitis. The results showed absolute values of flow, angular velocity, and pressure that it could deliver for use in APD therapies. Finally, the construction of the APD equipment is presented generally, showing the electronic and mechanical components that constitute it.
Effect of Pelvic Floor Muscle Training Using Pressure Biofeedback on Pelvic Floor Muscle Contraction and Trunk Muscle Activity in Sitting in Healthy Women
Authors: Ko Min-Joo , Koo Min-Suk , Jung Eun-Joo , Jeong Won-Jeong , Oh Jae-Seop
Published: March 2022

Pelvic floor muscle training (PFMT) has been recommended as the first choice as one of the effective methods for preventing and improving urinary incontinence (UI). We aimed to determine whether pressure biofeedback unit training (PBUT) improves short term and retention performance of pelvic floor muscle contraction. The muscle activities of the external oblique (EO), transversus/internal oblique (TrA/IO), multifidus (MF) and the bladder base displacement were measured in the verbal feedback group (n = 10) and PBU group (n = 10) three times (baseline, post-training, and at the 1-week follow-up). Surface electromyographic activity was recorded from the EO, TrA/IO, and MF muscles. The bladder base displacement was measured using ultrasound. The results were analyzed using two way mixed ANOVA. The bladder base displacement may have elevated more in the PBU group than in the verbal feedback group due to decreased TrA/IO activity. These findings indicate that PBUT is a better method than verbal feedback training.