bluuebunny/crossref_metadata_2025 huggingface dataset:
26. GAMBARAN HISTOPATOLOGIS GINJAL TIKUS PUTIH (Rattus novergicus) JANTAN YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERI EKSTRAK DAUN SERNAI (Wedelia biflora) (Histopathology of Male Rat (Rattus novergicus) Kidney Infected with Trypanosoma evansi and Treated with Sernai Leaves Extract (Wedelia biflora))
Authors: Fahrimal Yudha , R Rahmiwati , Aliza Dwinna
Published: September 2021

This research was aimed to study histopathology of rat kidney infected with Trypanosoma evansi after administration of sernai leaves extract (Wedelia bifolora). The samples used were kidneys of 25 male rats strain Wistar which were equally divided into 5 treatment groups. Group P1 was uninfected and untreated control, group P2 was infected with 1x103 T. evansi but not sernai leaves extract, rats from groups P3, P4, and P5 were infected with 1x103 T. evansi and given sernai leaves extract with the dose of 30 mg/kg bw, 45 mg/kg bw, and 60 mg/kg bw respectively. The sernai extract was administered orally using a stomach tube for 3 consecutive days. The next day after last treatment, all rats were sacrificed and necropsied. Kidneys were collected for histopathological examination such as degeneration and necrosis of tubules cells, convoluted contortus duct lumen, and adhesion and atrophy of glomerulus. Sernai leaves extract with the dose of 45 mg/kg bw was the most effective dose to prevent kidney damage due to T. evansi infection.
21. KAJIAN HISTOKIMIA SEBARAN KARBOHIDRAT PADA KELENJAR MANDIBULARIS DAN KELENJAR LINGUALIS AYAM PETELUR (Gallus sp.) Histochemical Study of Mandibular and Lingual Glands Carbohydrate Distribution in Layer (Gallus sp.)
Authors: H Hamny
Published: September 2021

The objective of this study was to investigate the distribution of carbohydrate in the mandibular and lingual glands of layer in difference age groups histochemically.The study was observed the mandibular and lingual glands from nine layers which grouped into three groups namely starter aged 40 days old, productive aged seven months old, and post-productive aged 1.5 years old. Each group consisted of three layers. A small piece of mandibular and lingual glands were fixed in Bouin solution for subsequent processed into histological preparation.The distribution of carbohydrate in both of glands detected by alcian blue staining (AB) pH 2.5 and periodic acid schiff (PAS). The result showed that mandibular and lingual glands of the three group layers reacted positively to AB pH 2.5 and PAS which found in the cytoplasm of cell and in the secretion on lumen. In the cytoplasm, the mandibular and lingualis reacted positively to AB pH 2.5 and PAS with the intensity ranging from weak reaction(+) to strong (+++), while in the lumen of glands showed negative reaction (-) to positive with the intensity of weak (+) to strong (+++). In mandibular gland, the acid carbohydrates were found with strong intensity (+++) in the productive and post-productive layer, while neutral carbohydrates were found with the same intensity on starter, productive and post-productive layers. In the lingual gland, the acid carbohydrates found with strong intensity in the three groups of layers while the neutral were found with strong intensity (+++) in the starter and post-productive layer. Positive reaction to AB pH 2.5 and PAS indicated that both of the salivary glands contain the neutral and acid carbohydrate. ____________________________________________________________________________________________________________________ Key words: mandibular gland, lingual gland, acid carbohydrate, neutral carbohydrate, layer
11. EFEK PENAMBAHAN AMPAS KEDELAI YANG DIFERMENTASI DENGAN Aspergillus niger DALAM RANSUM TERHADAP HISTOMORFOMETRI VILI USUS HALUS AYAM KAMPUNG (Gallus domesticus) The Effect of Aspergillus niger-Fermented Soybean Residue Supplementation in Ration the Histomophometry of Local Chicken (Gallus domesticus) Small Intestine
Authors: Sari Masyitah Nafli
Published: September 2021

The aim of this research was to find out the effect of Aspergillus niger-fermented soybean residue in ration on the histomorphometry of the small intestine villi of local chickens (Gallus domesticus).This study used a completely randomized design. Nine female chicken aged 15-16 months were divided into three groups. Each group consisted of three chikens. All chicken were given 324-2 ration and water ad libitum. Group P1 (control group) was given 100 g ration each day, group P2 was given a combination of 85 g ration and 10 g unfermented soybean residue everyday whereas group P3 was given 85 g ration and 10 g Aspergillus niger-fermented soybean residue everyday. Treatment was performed for 30 days. On day 31, chickens were slaughtered and subsequently three parts of the small intestine that consists of duodenum, jejunum, and ileum were collected and fixed in 10% neutral buffered formaline (NBF) solution for histological preparation. To measure the surface area of three regions of intestine, all histological sections were stained with Masson’s trichrome Goldner's modification. Based on the statistical analysis, there was no difference in height of villi among three parts of small intestine (P0.05), but there were statistical different (P0,05) in ileum basal and duodenum apical width of three groups after supplementation with fermented soybean residue and non-fermented. Based on the results of this experiment, it could be conluded that supplementation non-fermentation and the fermentation of soybean residue with A. niger has no impact on the height of vili ileum, but it increase the width of vili ileum and apical vili duedenum intestin of local chickens. ____________________________________________________________________________________________________________________ Key words: chicken, soybean residue, fermentation, small intestine
9. Total colony of cellulolitic bacteria in the rumen of aceh cattle
Authors: Safika Safika , Matondang Siti Wardianah , Darmawi Darmawi , Abral Mahdi , Erina Erina , Jalaluddin M
Published: September 2021

The aim of this study was to count the total sum of cellulolitic bacteria colony in  aceh cattle rumen. The sample used was aceh cattle rumen liquid which taken from salughtered house in Banda Aceh. Aceh cattle rumen liquid were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Petri dish was entered to candle jar to be incubated in an incubator with temperature 37oC about 96 hours. Colony of cellulolitic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of colony), counting total of bacteria which was grown, and Gram staining. The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil. The average number bacteria of total bacteria in the cattle rumen liquid was 4,81 x 105 cfu/ml
10. Isolation and Identification of Lactic Acid Bacteria Genus Pediococcus from Sumatran Orangutan (Pongo abelii) faeces at Kandi Zoo and Kinantan Zoo West Sumatera
Authors: Reimena Resti , Erina Erina , Darniati Darniati , Fakhrurrazi Fakhrurrazi , Darmawi Darmawi , Budiman Hamdani
Published: September 2021

This research has purposes to isolating and identifying the lactic acid bacteria genus Pediococcus in sumatran orangutan (Pongo abelii) feaces. Feaces are isolated from one orangutan at Kandi Zoo and two orangutans at Kinantan Zoo. The isolating of the lactic acid bacteria was done by using streak plate method with the MRSA (de Man Rogosa Sharpe Agar) specific media. Identification was done by the morphological observing, Gram stain, catalase test, oxidase test and biochemical test. The result showed that one isolate feaces from Kandi Zoo has cocci shaped with the isolate prediction approaches genus Pediococcus sp. The characteristic features of isolate are cocci shaped, Gram positive, tetrad cell structure, nonmotile, catalase negative, and oxidase negative. Based on these characteristics, it can be concluded that there is a lactic acid bacteria coloni which grouped into genus Pediococcus in male orangutan feaces from Kandi Zoo
2. Glucose levels of Canis familiaris Urine in Lamposi Tigo Nagori Payakumbuh Using Semiquantitative Striptest
Authors: Amalia Fhatania , Roslizawaty Roslizawaty , Rusli Rusli , Sayuti Arman , Hasan M , Amiruddin Amiruddin , Zuraida Zuraida
Published: September 2021

The aim of this study was to detect the glucose in urine of Canis familiaris as a medical record and  a supporting early diagnostic. Urine of thirty local dogs collected from Lamposi Tigo Nagori, Sumatera Barat. Before feeding, urine was collected individually in the morning two times. This study was doing on January 2016. The strip test was soaked in urine (5-10 ml) for 30 second directly. Data of glucose was analyzed using 4 scale discoloration of semi quantitative striptest. The result showed that glucose was not detected on 25 dogs out of 30 of  C. familiaris. Amount of 5 C. familiaris were detected the presence of glucose with small (100 mg/dl) scale. We concluded that the levels of glucose C. familiaris urine performed on the tolerance limits of glucose level.
12. Lipid profile in the Blood of the Sumatran Elephant (Elephas maximus sumatranus) in Elephant Conservation Center (ECC) Saree, Aceh Besar
Authors: Kamaruddin Mahmudi , Azhar Al , Sayuti Arman , Zuhrawati Zuhrawati , Asmilia Nuzul , Wahyuni Sri
Published: September 2021

This study was done to investigate lipid profile in the blood of Sumatran elephants at the ECC of Saree. In this stud blood samples were drawn from the auricular vein of 8 sumatran elephants at the ECC. Reasurement of total cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL), and triglicerida (TG) was carried spectrophotometrically by the commercial service of research laboratory in Banda Aceh. The results showed that the average concentrations of total cholesterol, LDL, HDL, and TG in the blood of Sumatran elephants were 155,86±54,39 mg/dL, 40,13±3,40 mg/dL, 101,13±46,16 mg/dL, dan 84,89±32,86 mg/dL. It can be concluded that the average concentrations of the lipid profile in the blood of the sumatran elephants at the ECC in Aceh were higher than that of reported in other subspecies of Asian elephant
16. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract
Authors: Aulia Rizki , Sugito Sugito , Hasan M. , Karmil T. Fadrial , Gholib Gholib , Rinidar Rinidar
Published: September 2021

The aim of this research was to determine the effect of giving neem leaf extract combined with jaloh towards the total of leukocytes and differential leukocyte in broilers that was infected with Eimeria tenella. This research samples were 20 Cobb stain broilers 14 days old. Design the research was complete randomized design with five treatments and each treatment consists of four repetitions. First treatment (P1) as negative control which was only given mineral water; second treatment (P2) as positive control was is given 5 mg/L anti-stress commercial in drinking water; Third treatment (P3) was given 1000 mg/L Jaloh extract; Fourth treatment (P4)was given 250mg/L neem extract; and fifth treatment (P5)was given 1000mg/L jaloh extract combined with 250mg/L neem extract. Anti-stress commercial and extract treatment dissolved in drinking water. The treatment start from 08.00 until 18.00 for ten days. Next in the 11th day (chicken 25 days old)was inoculated Eimeria tanella sporulatif as much as 1 x 104 ookista/ml orally. Then the blood was taken sampling when the chicken 30 days old (five days after infected). Blood sampling was done in the brachial vein. Furthermore, the number of leukocytes and differential leukocyte.Was calculated data were analyzed using complete randomized design with the help of SPSS for Windows 1.8 program. The result of this research showed that giving jaloh and neem leaf extract were not giving significant effect (P0,05) towards the number of leukocyte as well as differential leukocyte of broilers that was infected with Eimeria tenella. The conclusion of this research were giving jaloh leaf extract and neem for 10 days was not giving significant effect towards number of leukocytes and differential leukocyte broilers that infected with Eimeria tenella
4. The Value of Erythrocytes, Hemoglobin, and Hematocrit of Mice (Mus musculus) Exposed to Cigarette Smoke and Given Red Watermelon Extract (Citrullus vulgaris)
Authors: Heryanita Yola , Rusli Rusli , Rosmaidar Rosmaidar , Zuraidawati Zuraidawati , Rinidar Rinidar , Asmilia Nuzul , Jalaluddin Muhammad
Published: September 2021

The aim of this research was to investigate the effect of red watermelon (Citrullus vulgaris) extract to the value of erythrocytes, hemoglobin, and hematocrit of mice (Mus muscullus) exposed to cigarette smoke. This study followed direct complete randomized design by using 12 males’ mice which divided into 4 groups. The treatment group is divided by the negative control group which given 0,5 ml of aquadest, the positive control group which exposed to cigarette smoke and 0,5 ml of aquadest. The treatment group I was exposed to cigarette smoke and given red watermelon extract dose 22 mg/Bw mice. The treatment group II was exposed to cigarette smoke and given red with watermelon extract dose 44 mg/Bw mice. The exposure to cigarette smoke and the given of red watermelon extract were conducted for 30 days. The blood taking was performed on day 31 in the Plexus retroorbital.  Furthermore, the number of erythrocytes, hemoglobin and hematocrit were calculated. The data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. The results of this study showed that the extract of red watermelon for 30 days showed a highly significant effect (P 0.01) to the number of erythrocytes, hemoglobin, and hematocrit of mice. It is concluded that free radicals contained in the cigarette can cause a hemoglobin desaturation, increased blood viscosity, oxidative stress and red watermelon extract can minimize the damage.
3. The Effect of Audio Recording for Playback Experiment to the Alteration of Orangutan’s Territorial Behavior
Authors: Nazif Rahmat , Gholib Gholib , Rahmi Erdiansyah , Sayuti Arman , Lubis Triva Murtina , Balqis Ummu
Published: September 2021

The aim of this study was to find an alternative method to prevent the conflict between the orangutan and human with the utilization of audio recording as biofence. The location of this study was in Leuser National Park on Bukit Lawang, Langkat, North Sumatra using 16 orangutans as the subject of this study. The method used in this study was a simulation and playback experiment which were using long call, spontaneous call, predator voice, and siren sound in the audio recording of non-alpha orangutans. The respond was observed using binocular and recorded using camera recorder. The data obtained were analyzed using Krustal Wallist test. The result showed that 87% of orangutan showed an interest in the attractor, on the other hand, 13% showed disinterest to the attractor. The facial expression shown by orangutan was fear, submission, aggression, and worry. The statistical analyzing result showed that long call and the spontaneous call was very significant (P0.01), while the predator and siren voice were insignificant (P0.05). The result can be concluded that long call and spontaneous calls showing the effective result to the alteration of territorial behavior on orangutan and have a potential as a biofence that is used as an alternative method to prevent the conflict between orangutan and human.
16. The Effect of Indigofera sp. Leaf on the Protein Level of Rabbit Meat (Orictolagus sp)
Authors: Iqwal Yuza Al , Rastina Rastina , Harris Abdul , Ferasyi T. Reza , Azhar Al , Akmal Muslim
Published: September 2021

This study aimed to determine the effect of Indigofera sp. leaf to increase protein content of rabbit meat (Origtolagus sp). This study used a complete randomized design, consisted of 3 treatments and 3 replications. The samples were nine local male rabbits aged two months. P0 was control group which was given commercial feed, P1 was given 80% commercial feed and 20% Indigofera sp. pellet leaf, and P2 was given 70% commercial feed and 30%  Indigofera sp. pellet leaf. The feeding and drinking was conducted at 07:00 to 08:00 am and at 04:00 to 05:00 pm ad libitum. Protein content was measured using Kjeldahl method after eight weeks of treatments. The data was analyzed using Analysis of Variance (ANOVA) with SPSS v16.0 for Windows. The results showed that mean value (±SD) of meat protein for P0, P1 and P2 were 18.79+0.81%, 19.40+0.33% and 20.88+0.74%, respectively. We can conclude that the leaves of Indigofera sp. was highly significant (P0,01) in increasing protein level of rabbit meat at a concentration of 30%.
18. The Effect of Sipatah-Patah (Cissus quadrangularis Salisb) Extract on Mandible Density of Ovariectomized Rats (Rattus norvegicus)
Authors: Maghfirah Indah , Jalaluddin Muhammad , Masyitha Dian , Sabri Mustafa , Gani Fadli A , Herrialfian Herrialfian
Published: September 2021

The aim of this research was to identify the effect of sipatah-patah (Cissus quadrangularis Salisb) leaf extract on mandible density of ovariectomized rats (Rattus norvegicus). The samples were 12 female rats which were divided into 4 treatment groups with 3 replications. K0 consisted of ovariectomized rats without Cissus quadrangularis Salisb exstract, wheares K1, K2 and K3 consisted of ovariectomized rats  and were given Sipatah-patah extract with 500mg/kg body weight, 700/mg/kg body weight and 900mg/kg body weight for 30 days. On 31st day, rats were euthanized with cloroform and mandible bones were collected for histological preparation. Parameters observed were bone structures (osteosit, osteoblast, osteoclast and trabekula). The results showed that in K0 group, bone density was decreased and became thinner, osteoclast cells were found on trabekula, and low density of active and passive osteoblast were also detected. The treatment group (K1 and K2) showed an improvement stucture of trabekula,where the amount of osteoclast were lower than K0, as well as the amount of active osteoblast, although a lot of passive osteoblast were still found. The treatment group (K3) showed an improvement as the most dense trabekula structure with large amount of active osteoblast cell density without the existance of osteoclast compared to other grups. In this research, it would be concluded that the adding sipatah-patah extract with a dose of 900 mg/kb body weight showed a higher number of active osteoblasts and trabekula density compared to the control groups, K1, and K2 on ovariectomized mandible bone rats
33. Efficacy of Jatropha(Jatrophacurcas L.) Sap Cream in Inflammation Phase of Wound Healing on Mice Skin
Authors: Hidayati Hidayati , Salim Muhammad Nur , Balqis Ummu , Masyitha Dian , Iskandar Cut Dahlia , Darmawi Darmawi , Harris Abdul
Published: September 2021

This study aim to know the efficacy of the jatropha sap cream toward the inflammatory phase of wound healing on the skin of mice. This study used 9 male mice, with 25-40 grams weights and 2-3 months old.  Male mice receiveddivided into 3 groups, and3 deuteronomy.The cut is done in the back as 2 cm long. Wound care is done twice a day at 08.00 am and at 18.00 pm for 3 days. The treatment I (PI) cuts smeared a cream base (as a negatif control); treatment II (PII) smeared 10% jatropha sap cream; and the treatment III (PIII) smeared 0.1% sulfadiazine  (as a positive control).Created a skin sample preparation microtechnic stained with hematoxylin-eosin (HE). The result on the number of infiltration of inflammatory cells and neovascular on PI; PII; and PIII were 402,00 ± 7,21 and 14,00 ± 5,57; 248,67 ± 1,53 and 34,67 ± 7,51; and 343,00 ± 8,89 and 28,00 ± 5,00  (P0,01). Based on the result it be concluded the treatment of 10% jatropha sap cream can speed up the process of the inflammatory phase of wound healing in the skin of mice.
Isolation and Identification of Genus Salmonella and Shigella from Sumatran Orangutan (Pongo abelii) Feces in Orangutan Reintroduction Center, Jantho
Authors: Rahmi Erdiansyah , Agustina Dina , Jamin Faisal
Published: September 2021

This study was done to identified bacteria of genus Salmonella and Shigella from sumatran orangutan (Pongo abelii) feces. Nine semi wild orangutan fecal samples were obtained in Orangutan Reintroduction Center, Cagar Alam Pinus Jantho, Aceh Besar. The samples were processed using Carter methode. The selective media used were Salmonella Shigella Agar (SSA), Eosin Methylen Blue (EMB), and MacConkey Agar. The separated colonies which grawn in SSA media were observed for their morphology, and followed by Gram staining methode then continued with biochemical test such as Indol, Methyl Red (MR), Voges Proskauer (VP), Sulfid Indol Motility (SIM), Simmons Citrate (SC), and sugar test such as glucose, sucrose, lactose, and manitol. From nine samples, one orangutan was infected with Salmonella and another one was infected with Shigella. All of the samples were identified with Escherichia. In Conclusion, orangutan lived at Reintroduction Center, Jantho infected with Salmonella and Shigella bacteria.Key words: feces, orangutan, Salmonella, Shigella
The Effect of Bull Age and Ejaculation Frequency on Quality of Aceh Bull Spermatozoa
Authors: Melita Dini , Dasrul Dasrul , Adam Mulyadi
Published: September 2021

The purpose of this research was find out the effect of age and ejaculation frequency on quality of Aceh bull spermatozoa.. This research was conducted at Frozen Semen Prossesing Laboratorium, Saree, Aceh Besar from Januari to April 2013. Six healthy aceh bulls were divided into two groups of age, those were 3.0-4.0 years old; and 4.0-5.0 years old. Three numbers of bull at each group were assumed as the replication. Semen was collected once a week using artificial vagina and collected until third semen ejaculation. After collecting, volume, pH, color, consistency, concentration, motility, mortality, and abnormality of spermatozoa were immediately measured. This data was analyzed by analysis of variance (ANOVA) two-way pattern, followed by Duncant test. The result showed that the evaluation of bull semen at 3.0-4.0 years old and 4.0-5.0 years old affect significantly (P0.05) on semen volume and spermatozoa concentration and had no contribute significantly on motility, mortality and abnormality of spermatozoa,while ejaculation frequency effect significantly (P0.05) on semen volume, concentration, and abnormality spermatozoa, but had no effect significantly on motility and mortality (P0.05). It is concluded that aged and ejaculation frequency effect significantly on quality of aceh bull fresh spermatozoa.Key words: ejaculation frequency, quality of spermatozoa, aceh bull, age
The Effect of Vitamin C Addition in Andromed® Dilution on Procentage of Motility and Spermatozoa Intact Plasma Membrane of Aceh Bull After Freezing
Authors: Aslam Hasan Al , Dasrul Dasrul , Rosmaidar Rosmaidar
Published: September 2021

This study aimed to determine the effect of vitamin C addition in andromed® dilution on procentage of motility and intact plasma membrane post thawing of aceh bull spermatozoa. This research used fresh semen aceh bull were collected using artificial vagina and divided into four treatments.Control treatment (P0) semen without the addition of vitamin C, first treatment (P1) semen plus vitamin C 0.5 g/100 ml of dilution, second treatment (P2) semen plus vitamin C 1.0 g/100 ml of dilution, third treatment (P3) semen plus vitamin C 2.0 g/100 ml of dilution. The experiment design used was completely randomized design (CRD). Data was analyzed using ANOVA (Analysis of Variant) and followed by Duncan’s multiple range (Duncan’s Multiple Range Test) 5% confidence interval. The result showed that the addition of vitamin C influence the motility and intact plasma membrane percentage of aceh bull spermatozoa after thawing. The addition of vitamin C 0.5 g/100 ml and 1.0 g/100 ml increase motility and intact plasma membrane percentage, while vitamin C 2.0 g/100 ml decrease motility and intact membrane plasma percentage after thawing.Key words: intact plasma membrane, motility, aceh bull, spermatozoa, vitamin C
Toxicity Test of Ethanol Extract Ant Plant Local Aceh (Mymercodia sp) Method of BSLTLarvae Shrimp Artemia salina Leach
Authors: F Frengki , R Roslizawaty , Pertiwi Desi
Published: September 2021

Toxicity test and phytochemical screening of ant plant ethanol extract were done. The toxicity test was done by employing the Brine Shrimp Lethality Test (BSLT) method. Sample of ant plant taken from the Aceh Besar forest have been extracted with ethanol. The sample was thenconcentrated using a rotary evaporator until a crude extract was obtained. The extract was tested by toxicity to larva Artemia salina L. Thetoxicity test for larvae Artemia salina Leach have been done by employing the Brine Shrimp Lethality Test (BSLT) method at four concentrationlevels, i.e: 250 ppm, 125 ppm, 50 ppm, and 25 ppm. The result of the research showed that ethanol extract of ant plant Aceh had acute toxicityby LC value 61.11 ppm. The extract had identified were fenolik, saponin, steroid and terpenoid.____________________________________________________________________________________________________________________Key words: rine Shrimp Lethality Test, LC5050, Ant Plant, Aceh, Bphytochemical Screening
Effect of Vitamin E Supplementation in the Low Density Lipoprotein Consentration and Histopatologic Changes of the Wistar Rats Aorta Given High Fat Diet
Authors: Daud Razali
Published: September 2021

The aims of this research were: a) to determine the protective effect of vitamin E in the low density lipoprotein concentration and aorta , b) to give information, especially in the relationship between the high fat diet and the incidence of cardiovascular diseases. Twenty five male wistarrats weighing 200–250 grams at the age of 3 months were used in this study. Completely randomized design was implemented for thisexperiment. The rats were acclimated for a week and fed with CP 521 (basal). The rats were then randomly allotted into 5 groups, 5 sampleseach. The rats were fed basal (CP 521) as control (G I), high fat diet (CP 521 + palm oil 10%) + 50 IU vitamin E (G II), high fat diet fat + 100IU vitamin E (G III), high fat diet + 200 IU vitamin E (G IV) and high fat diet + 400 IU vitamin E (G V). Diets were given about 10% of bodyweigh and it’s fed up every day. Water was given ad libitum. Based on statistically analysis showed that very significance (P0,01) between thetreatment. Result indicated that given high fat diet + 400 IU vitamin E could apparently reduce LDL concentration, Lesion in the aortaconsisting of hemorrhagic and hyperemic were found in all rats receiving high fat diet supplemented with less than 400 IU vitamin E. The ratsreceiving high fat diet + 400 IU vitamin E (G V) the aorta were normal. It was concluded that the diet containing high fat and high vitamin Esupplementation (400 IU ration) in the diet could protected the incidence of atherosclerosis.____________________________________________________________________________________________________________________Key words: atherosclerosis, high fat diet, vitamin E,
Identification of Parasites in Snakehead Fish (Channa striata) in Meunasah Manyang Village Lhoknga Subdistrict Aceh Besar
Authors: Umara Adil , Bakri Muttaqien , Hambal Muhammad
Published: September 2021

The study aimed at identifying the types of parasites that infect snakehead (C. striata) fish as well as to determine the prevalence of the parasite species. A total of 51 snakehead fish was captured using nets and traps then examined at Parasitology Laboratory of Veterinary Medicine Faculty Syiah Kuala University Banda Aceh. Ectoparasites inspection was done by observation of the outer body organs as well as by making the mucus smear examined under a microscope, while endoparasites inspection was done by observation on the surface of the digestive tract and heart, and also thin and thick boold smear. The study revealed that two types of parasites that infect snakehead fish in irrigation Cot Balee were Pallisentis nagpurensis and Trichodina spp. It also found larvae obtained from the intestinal villi scrapings that have not been identified. A total of 96% snakehead fish infected by P. nagpurensis, 6% infested with Trichodina spp., and 10% infested by unidentified larvae.Key words: parasite, snakehead fish (Channa striata), Palllisentis nagpurensis, Trichodina spp, prevalence
Early Pregnancy Diagnosis Using Milk Progesterone Kits in Etawah Cross Goat (Capra hircus)
Authors: Nova Milona Elsa , Riady Ginta , Melia Juli
Published: September 2021

This study aims to determine the effectiveness and accuracy of milk progesterone kit as an early pregnancy diagnosis tool for Etawah cross goat. This study used 9 lactating female goats with weight in the range of 35-51 kgs and the ages ranging from 2 to 2.5 years old. Eight goats were subject to estrous synchronisation protocol using progesterone implant (CIDR-G) followed by prostaglandin administration and one goat was left to show natural estrous. Early pregnancy diagnosis using milk progesterone kits was carried out on day 18 until 22 following services. The accuracy of the kits for early pregnancy diagnosis was subsequently confirmed using ultrasonography (USG) examination on day 35 following services. The results of this study showed that estrous response by synchronization was 50%. Whereas one female goat showed estrous sign naturally and was subject to pregnancy diagnosis procedure using the milk progesterone kits after natural mating. Pregnancy diagnosis using milk progesterone kits and subsequent confirmation using USG was carried out in five goats (4 synchronized females and mated and 1 naturally estrous female and mated naturally). All 5 female goats showed negative results following pregnancy diagnosis using the kits throughout the whole 5 days examinations. While diagnosis of pregnancy as confirmed by USG showed one goat (number 6) positive. It can be concluded that milk progesterone kits on dairy cows are less effective for diagnosing pregnancy on Etawah cross goat. The accuracy of the kits for pregnancy diagnosis of not-pregnant status is 80% after confirmation using USG.Key words: CIDR-G, Ettawah goats, milk progesterone kits, ultrasonography
IDENTIFIKASI PARASIT PADA IKAN NILA (Oreochromis niloticus) DI IRIGASI BARABUNG KECAMATAN DARUSSALAM ACEH BESAR (Identification of Parasites on Nile Tilapia (Oreochromis niloticus) Fish Collected from Barabung Irigation Darussalam Aceh Besar)
Authors: Lianda Nova , Fahrimal Yudha , Daud Razali , Rusli Rusli , Aliza Dwinna , Adam Mulyadi
Published: September 2021

This study was aimed to identify parasite types that infected Nile tilapia (Oreochromis niloticus) fish collected from Barabung irigation Darussalam, Aceh Besar. A total of 26 Nile tilapia fish were collected from Barabung irigation then examined for the presence of ectoparasite and endoparasite at Parasitology Laboratory, Veterinary Medicine Faculty, Syiah Kuala University Banda Aceh. The ectoparasite examination was conducted by observed the external body part of fish macroscopically and microscopically. While endoparasite examination was conducted by observed the inner surface of digestive track. The results showed that four types of parasites were found namely Dactylogyrus sp., Gyrodactylus sp., Camallanus sp., and P. nagpurensis. Overall,73.1% Nile tilapia fish was infested by Dactylogyrus sp., 11.3% was infested by Gyrodactylus , 10 % was infested by Camallanus, and 5% was infested with P. nagpurensis.Key words: endoparasite, ectoparasite, tilapia fish
Exclusion Criteria of Breast Cancer Clinical Trial Protocols: A Descriptive Analysis
Authors: Wan Clara , Caston Nicole E. , Ingram Stacey A. , Rocque Gabrielle B.
Published: September 2021

Purpose: 3-8% of US adults with cancer are enrolled in a clinical trial due to various barriers to enrollment. The purpose of this study is to evaluate the variability of eligibility criteria, which currently have no standard guidelines.Methods: This descriptive analysis utilized all therapeutic breast protocols offered at the University of Alabama at Birmingham between 2004-2020. Exclusion criteria were abstracted using OnCore and ClinicalTrials.gov. Laboratory values included liver function tests and hematologic labs. Comorbid conditions included congestive heart failure, cardiovascular disease, central nervous system (CNS) metastases, and prior cancer history. Comorbid conditions were further analyzed by amount of time protocols required participants to be from diagnosis or exacerbation-free.Results: 102 protocols were eligible. Among liver laboratory values, bilirubin (78%) was included in most protocols ranging from institutional upper limit of normal (ULN) (9%) to 3xULN (2%), with 1.5xULN (56%) being most common. Similar variability was observed in alanine transaminase and aspartate transaminase. Among hematological labs, 82% of protocols defined a lower limit of acceptable absolute neutrophil count ranging from 500μL (1%) to 1,800μL (1%), with 1,500μL (64%) being most common. Of the comorbid conditions, exclusion criteria varied for congestive heart failure (49%), an acute exacerbation of cardiovascular disease (80%), CNS metastases (59%) and a prior cancer (66%). The allowable timeframe varied between protocols for cardiovascular disease and prior cancer.Conclusion: Substantial heterogeneity was observed across laboratory values and comorbid variables among protocols. Future research should focus on defining standardized eligibility criteria while allowing for deviation based on drug specificity.
Liver Transplantation in Children and Impact of Intra-operative Goal Directed Therapies on Postoperative Outcome
Authors: Kumba Claudine
Published: September 2021

Background: Liver transplantation is a hemorrhagic surgery with high postoperative complication rates in terms of organ dysfunction and infections. We conducted a retrospective observational monocentric study that had the objectives of determining predictors of postoperative outcome in children. In the initial cohort, 19 children underwent liver transplantation. A description of the postoperative outcomes of these patients was undertaken.Objective: To describe intraoperative and postoperative outcomes in children undergoing liver transplantation and to propose intraoperative management implementation measures for postoperative outcome improvement.Methods: Secondary analysis of liver transplanted patients included in the initial study.Results: There were 19 patients who underwent liver transplantation with a median age of 31[2-154] months. Five patients had re-operations (26.3%). Fourteen (73.7%) had intraoperative and/or postoperative complications. Five (26.3%) had intraoperative hemorrhagic shock. One (5.3%) had postoperative respiratory failure, one (5.3%) had postoperative multiple organ failure, two (10.5%) had postoperative neurologic failure and five (26.3%) had cardio-circulatory failure. One (5.3%) had postoperative pulmonary sepsis, five (26.3%) had postoperative abdominal sepsis, and six (31.6%) had postoperative septicemia. Intra-operative transfusion rate was 100%. In-hospital mortality rate was 10.5% (two patients).Conclusion: There were multiple postoperative adverse outcome predictors. Optimal intraoperative patient management with transfusion guided by point-of-care viscoelastic devices and goal-directed fluid and hemodynamic therapy with validated parameters and tools in children is a possible pathway to upgrade postoperative evolution in pediatric liver transplantation.
Neuroinflammatory Cytokine Response, Neuronal Death and Microglial Proliferation in the Hippocampus of Rats During the Early Period After Lateral Fluid-Percussion-Induced Traumatic Injury of the Neocortex
Authors: Komoltsev Ilia G. , Tretyakova Liya V. , Frankevich Stepan O. , Shirobokova Natalia I. , Volkova Aleksandra A. , Butuzov Alexey V. , Novikova Margarita R. , Kvichansky Alexey A. , Moiseeva Yulia V. , Onufriev Mikhail V. , Bolshakov Alexey P. , Gulyaeva Natalia
Published: September 2021

Time course of changes in neuroinflammatory processes in the dorsal and ventral hippocampus was studied during the early period after lateral fluid-percussion-induced neocortical traumatic brain injury (TBI) in the ipsilateral and contralateral hemispheres. In the ipsilateral hippocampus, neuroinflammation (increase in expression of pro-inflammatory cytokines) was evident from day 1 after TBI and ceased by day 14, while in the contralateral hippocampus it was mainly limited to the dorsal part on day 1. TBI induced an increase in hippocampal corticosterone level on day 3 bilaterally and an accumulation of Il1b on day 1 in the ipsilateral hippocampus. Activation of microglia was observed from day 7 in different hippocampal areas of both hemispheres. Neuronal cell loss was detected in the ipsilateral dentate gyrus on day 3 and extended to the contralateral hippocampus by day 7 after TBI. The data suggest that TBI results in distant hippocampal damage (delayed neurodegeneration in the dentate gyrus and microglia proliferation in both the ipsilateral and contralateral hippocampus), the time course of this damage being different from that of the neuroinflammatory response.
A Dissection of the Topographic Effects from Eurasia and North America on the Isentropic Meridional Mass Circulation in Northern Winter
Authors: Yu Yueyue , Ren Rongcai , Xia Xin , Liang Ruxue , Rao Jian
Published: September 2021

The topographic dynamical effect from Eurasia (EA_Topo) and North America (NA_Topo) on the winter isentropic meridional mass circulation (IMMC) is investigated using the WACCM. The independent effect of EA_Topo and that of NA_Topo, with the former much stronger, are both to strengthen the IMMC that is composed of the lower equatorward cold air branch (CB) and the upper poleward warm air branch in the extratropical tropopshere (WB_TR) and stratosphere (WB_ST). Further investigation of the individual contributions from changes in stationary vs. transient and zonal-mean flow vs. waves reveals that, due to the topography-forced mass redistribution, changes in the low-level meridional pressure gradient force a zonal-mean counter-clockwise/ clockwise meridional cell in the southern/northern side of topography. This weakens/strengthens the IMMC south/north of 30°N from the troposphere to lower stratosphere, acting as a dominant contributor to the IMMC changes south of 50°N. Meanwhile, the EA/NA_Topo-forced amplification of stationary waves constructively interacts with those determined by land-sea contrast, making the dominant/minor contributions to the strengthening of CB and WB_TR north of 50°N. The related increase in the upward wave propagation further dominates the WB_ST strengthening in the subpolar region. Meanwhile, transient eddy activities are depressed by EA/NA_Topo along with the weakened background westerly, which partly-offset/dominate-over the contribution from stationary flow in midlatitudes and subpolar region. The coexistence of the other topography (NA/EA_Topo) yields destructive mutual interferrence, which can weaken/offset the independent-EA/NA_Topo-forced meridional mass transport mainly via changing the zonal-mean as well as the downstream wave pattern of mass and meridional wind.
Sustainable Valorization of Olive Pomace Waste to Renewable Biofuels, Biomaterials and Biochemicals Via Pyrolysis Process: Experimental and Numerical Investigation
Authors: Aissaoui Mohamed Hechmi , Trabelsi Aïda Ben Hassen , Bensidhom Gmar , Ceylan Selim , Leahy James.J , Kwapinski Witold
Published: September 2021

This work demonstrates, experimentally and numerically, the potential of Olive Pomace Waste (OPW) to produce renewable biofuels (pyrolytic oil and gas), bio-chemicals (tars as source of bioactive molecules) and bio-fertilizers (chars) through slow pyrolysis. Experimental pyrolysis runs were conducted at 500, 600 and 700°C as final pyrolysis temperature, 15, 20 and 25°C/min as heating rate and 1h as residence time, in a fixed bed pyrolyzer. In the optimum pyrolysis conditions (600°C and 15°C/min), 33 wt.% of oil, 30.00 wt.% of char and 37 wt.% of gas were produced. Recovered pyrolytic oil presents good energy value (HHV between 15.96 and 20.94 MJ/kg) with a great bioactive potential. The released permanent gases show an interesting energy content (LHV up to 11 MJ/Kg) which emphasizes their application in a gas engine to provide renewable electricity in rural olive groves area. The recovered OPW biochar presents a high carbon (C 72.54 wt.%) and nutrients contents (up to 8.42 mg/g of Ca, up to 8.69 mg/g of K and up to 2.02 % of total N) which make it suitable for soil amendment and for long-term carbon sequestration. Kinetic study of OPW pyrolysis, performed using the Distributed Activation Energy Model (DAEM), gives an activation energy values ranging from 121.6 to 151.6 kJ/mol. The investigation of the OPW thermal behavior and reactivity under pyrolysis conditions is useful approach to design and operate slow pyrolysis process at commercial scale, which could be useful by farmers for OPW in olive fields.
Follicle-stimulating Hormone and Lipid Profile in Women Older than 55
Authors: Zhang Wen , Chen Yingchao , Zhang Xian , Chen Yi , Han Bing , Li Qin , Xia Fangzhen , Zhai Hualing , Wang Ningjian , Lu Yingli
Published: September 2021

Purpose. Follicle stimulating hormone (FSH) is associated with higher risks of metabolic syndrome and diabetes in menopausal women. We aimed to investigate whether FSH was associated with lipid profile in women older than 55 who were postmenopausal with a high probability.Methods. Our data were from Survey on Prevalence in East China for Metabolic Diseases and Risk Factors. 1795 women at the age of 55-89 were selected. Sex hormones and lipid profile were measured from morning serum. Linear and logistic regression analyses were used. Results. Lower FSH was associated with lower HDL-C, higher TG, TC/HDL-C ratio and LDL-C/HDL-C ratio (all P for trend<0.05), after adjusting for age and other sex hormones. Further adjustment for body mass index, diabetes and hypertension, the associations of FSH with lipid profile weakened, but the association of FSH quartiles with HDL-C and TC/HDL-C ratio was still significant (both P for trend<0.05). Compared with women in the highest FSH quartile, odds of low HDL-C (HDL-C <1.04mmol/L) in women in the lowest FSH quartile were 5.25 (95% confidence interval 1.60, 17.26) (P for trend <0.05) in fully adjusted model, and odds of TC≥6.22mmol/L, TG≥2.26mmol/L and LDL-C≥4.14mmol/L were not significant. LH did not show significant association with dyslipidemia.Conclusion. Lower FSH was associated with worse lipid profile in women older than 55. Diabetes, adiposity and hypertension mostly explained the association of FSH with TG and LDL-C/HDL-C ratio but only partially explained the associations of FSH with HDL-C and TC/HDL-C ratio.
Prognostic Significance of Carbohydrate Antigen 125 in Acute Heart Failure: A Prospective Comparative Study With N-terminal Pro-B-type Natriuretic Peptide
Authors: Zhang Ji , Li Wenhua , Cai Gaojun , Xiao Jianqiang , Hui Jie , Shi Ganwei , Yu Lei , Xue Sheliang
Published: September 2021

Background: In acute heart failure (AHF), elevated carbohydrate antigen 125 (CA125) and N-terminal pro-B-type natriuretic peptide (NTproBNP) have shown to correlate with adverse events. We sought to quantify their prognostic usefulness in predicting the 6-month combined endpoint of death/heart failure readmission.Methods: The study includes 352 patients admitted for AHF. The primary endpoint was 6-month combined endpoint of death/AHF rehospitalization. CA125 and NTproBNP were dichotomized according to the best cut-offs to predict 6-month primary endpoint. By multivariate Cox regression analysis, the independent association of CA125 and NTproBNP with the primary endpoint was assessed, and their incremental prognostic utility evaluated by net reclassification improvement (NRI) and integrated discrimination improvement (IDI) index. Results: A total of 47 (13.4%) deaths and 113 (32.1%) AHF rehospitalizations were identified at 6-month follow-up. The subjects with CA125≥39.7 U/ml and NTproBNP≥3900 pg/ml had significantly higher cumulative event rates (56.1% vs. 33.3% and 53.3% vs. 33.8%, both P<0.001). Elevated CA125 (HR 1.93; 95%CI [1.32-2.83]; P=0.001) was associated with higher HR than NTproBNP≥3900 pg/ml (HR 1.71; 95%CI [1.19-2.48]; P=0.004) after adjusting for established risk factors. Elevated CA125 still independently predicted adverse events when both CA125 and NTproBNP were entered together in the same multivariate model. Furthermore, risk reclassification analyses demonstrated significant improvements in NRI of 22.3% (P=0.014) and IDI of 2.7% (P=0.012) when adding CA125 to the base model + NTproBNP.Conclusions: Elevated CA125 and NTproBNP predicted adverse outcomes in AHF patients. CA125 added prognostic value to NTproBNP, and thus, their combination conferred greater predictive capacity.
Effects of Bacterial Organic Selenium, Selenium Yeast and Sodium Selenite on Antioxidant Enzymes Activity, Serum Biochemical Parameters, and Selenium Concentration in Lohman Brown-Classic Hens
Authors: Muhammad A. I. , Dalia A. M. , Loh T. C. , Akit H. , Samsudin A. A.
Published: September 2021

This study compares the effects of sodium selenite, selenium yeast, and enriched bacterial organic selenium protein on antioxidant enzyme activity, serum biochemical profiles, and egg yolk, serum, and tissue selenium concentration in laying hens. In a 112-d experiment, 144 Lohman Brown Classic hens, 23-wks old were divided into four equal groups, each has six replicates. They were assigned to 4 treatments: 1) a basal diet (Con), 2) Con plus 0.3 mg/kg feed sodium selenite (SS); 3) Con plus 0.3 mg/kg feed Se-yeast (SY): 4) Con plus 0.3 mg/kg feed bacterial enriched organic Se protein (ADS18) from Stenotrophomonas maltophilia bacteria. On d 116, hens were euthanized (slaughtered) to obtain blood (serum), liver organ, and breast tissue to measure antioxidant enzyme activity, biochemical profiles, and selenium concentration. The results show that antioxidant enzyme activity of hens was increased when fed bacterial organic Se (ADS18), resulting in a significant (P < 0.05) increase in serum GSH-Px, SOD, and CAT activity compared to other treatment groups. However, ADS18 and SY supplementation increase (P < 0.05) hepatic TAC, GSH-Px, and CAT activity, unlike the SS and Con group. Similarly, dietary Se treatment reduced total cholesterol and serum triglycerides concentrations significantly (P < 0.05) compared to the Con group. At 16 and 18 weeks, selenium concentration in hen egg yolks supplemented with dietary Se was higher (P < 0.05) than in Con, with similar patterns in breast tissue and serum. Supplementation with bacterial organic Se (ADS18) improved antioxidant enzyme activity, decreased total serum cholesterol and serum lipids, and increased Se deposition in egg yolk, tissue, and serum. Hence, organic Se may be considered a viable source of Se in laying hens.