bluuebunny/crossref_metadata_2025 huggingface dataset:
INITIATION OF THE FORMATION OF FIT VILLAGE AS AN EFFORT TO PREVENT METABOLIC DISEASES IN SINGOTRUNAN VILLAGE, BANYUWANGI
Authors: Herawati Lilik , Argarini Raden , Irwadi Irfiansyah , Setiawan Hayuris Kinandita , Wiyasihati Sundari Indah , Azmy Ulul
Published: July 2024

Metabolic disease is an epidemic phenomenon that affects both men and women from various ages, ethnic, and socio-economic groups. The term metabolic disease itself is an umbrella term that includes, among others: hypertension, type 2 diabetes mellitus, hyperlipidemia, obesity, and non-alcoholic fatty liver disease. Lifestyles such as poor nutrition, physical activity, and habits are risk factors that can increase the incidence of metabolic disease. Therefore, prevention of metabolic diseases is necessary, including for ordinary people. Metabolic disease, namely hypertension, is in 3rd place and diabetes is in 10th place, the most common disease suffered by residents of Singotrunan Village, Banyuwangi. In this regard, it is important to make efforts to prevent metabolic disease and even death due to complications from metabolic disease. This community service program is designed to create a fit village as a sustainable healthy lifestyle, initiated through health checks for screening, exercise training, education and discussions as encouragement to do regular exercise. The participants' pre-test and post-test results showed a significant increase in their level of knowledge (p=0.01). The results of the participant satisfaction test showed that 80.9% of participants said they were very satisfied and the rest (19.1%) said they were satisfied. It is hoped that the PKM activity to initiate a fit village in Singotrunan Village, Banyuwangi will provide motivation for residents to exercise regularly and can become a model village for residents of other areas.
The Impact of Confucian Culture on Corporate Environmental Governance
Authors: Wu Xiancong , Hao Meng , Wu Di , Skolnik Richard
Published: July 2024

Using A-share listed companies in Shanghai and Shenzhen from 2010 to 2019 and the density of Confucian temples and schools near sample company headquarters, this paper uses regression analysis to estimate the impact of Confucian culture on corporate environmental governance.  Enterprise environmental capital investment as a percent of total assets is the dependent variable used as a proxy for environmental governance. The log of the number of the number of Confucian temples and Confucian academies within a 200-kilometer radius of the company's registered location plus one is the independent variable. This study finds that the environmental capital expenditures increase by 0.007% for every unit increase in the independent variable. Similar results are found when the radius is expanded to 300 kilometers. The greater the influence of Confucian culture is, the more inclined enterprises are to participate in corporate environmental governance. Additionally, environmental governance information disclosure can be a key influence path for this correlation.  Further analysis finds that the relationship between Confucian culture and environmental governance is stronger with more experienced managers, a more developed institutional environment, and after 2015 following the People's Republic of China’s enactment of the Environmental Protection Law.  This study expands the literature on the factors that influence Confucian culture on corporate environmental governance, enriches the empirical evidence of the influence of the informal system of corporate environmental governance on enterprise decision-making, and confirms the modern commercial value of Confucian culture.
Dalbergia calcarea, a new species of Dalbergia (Fabaceae: Dalbergieae) from Lao PDR
Authors: Mattapha Sawai ,   Department of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand., Lanorsavanh Soulivanh , Lamxay Vichith , Souvannakhoummane Keooudone , Chanthavongsa Khamfa ,   Department of Biology, Faculty of Natural Sciences, National University of Laos, Lao PDR.,   Department of Biology, Faculty of Natural Sciences, National University of Laos, Lao PDR.,   Department of Biology, Faculty of Natural Sciences, National University of Laos, Lao PDR.,   Department of Biology, Faculty of Natural Sciences, National University of Laos, Lao PDR.
Published: July 2024

Dalbergia calcarea, a new species currently only known from the limestone mountains of Khammouan Province, central Laos, is described and illustrated. The key characters such as habit, colour and limb of standard, and number of ovules of the new species are discussed and compared with its closest relatives. A detailed description along with etymology, conservation status, illustrations and colour photographs are provided for a new species. This discovery increases the number of Laotian species in the genus Dalbergia to seventeen taxa.
L'image des Jeux dans le 7e art : esquisse d'une histoire fictionnelle
Authors: Gerville-Réache Hugo
Published: July 2024

Desde los comienzos del cine de Hollywood, las imágenes que documentan los Juegos han sido una fuente de inspiración. Un ejemplo de ello es la película Charlie Chan at the Olympics (Humberstone, 1937), en la que se proyectan imágenes de actualidad de los Juegos de 1936. Desde entonces, han surgido numerosos largometrajes similares, algunos de los cuales han llegado a un amplio público: juntos forman una constelación de imágenes cinematográficas, una proyección del mundo. Por un lado, los cineastas incluyen las imágenes captadas en el momento de los Juegos Olímpicos para apoyar su versión de la historia, y, por otro, el séptimo arte transforma las imágenes en símbolos narrativos que sirven para el avance de relatos. En esta perspectiva, tres grandes tipos de imágenes son retenidas aquí con el fin de esbozar una historia ficticia de los Juegos en el cine: acontecimiento, deportivo y moral.
L’influence de la télévision et de la radio publique sur les attentes sportives du public : l’exemple de Christine « Kiki » Caron lors de Jeux Olympiques de Tokyo en 1964
Authors: Philippe Marion
Published: July 2024

Nacida en 1948, Christine Caron se erigió en campeona al servicio de los proyectos de la sociedad gaullista. La televisión y la radio desempeñaron un papel importante en la conversión de esta figura en campeona, además de ídolo de la juventud. Plusmarquista en Francia, y luego en Europa, de los 100 y 200 metros espalda, Christine Caron es conocida porque logra hazañas deportivas, pero sobre todo porque representa un ideal de juventud y responde a las normas de la feminidad deportiva. Aun así, el público francés esperaba de ella que fuera una futura campeona olímpica. El 14 de octubre de 1964 quedó segunda. Para la televisión y la radio, fue un fracaso. A partir de un corpus de extractos radiofónicos y televisivos públicos de 1962 a 1964, se trata aquí de examinar el papel de los medios de comunicación en la construcción de las esperanzas francesas de una medalla de oro olímpica para Christine Caron en los Juegos Olímpicos de Tokio en 1964.
Medical science in Podhale – memoirs of Stanisław Snopek from the time of the Polish People’s Republic
Authors: Moskalewicz Marcin
Published: July 2024

This is a review of the book entitled “Medycyna, moja miłość” (“Medicine, my love”), which was written by an internist from the town of Nowy Targ, Stanisław Snopek. The book is not scientific in nature and is a piece of memoir literature.
Multiple myeloma survival in New South Wales, Australia, by treatment era to 2020
Authors: Feletto Eleonora , Luo Qingwei , Kelly Anna , Weber Marianne , Goldsbury David , Barron Katherine , Canfell Karen , Yu Xue Qin
Published: July 2024

Objective: Australia has relatively high multiple myeloma (MM) incidence and mortality rates. Advancements in MM treatment over recent decades have driven improvements in MM survival in high-income countries; however, reporting in Australia is limited. We investigated temporal trends in population-wide MM survival across 3 periods of treatment advancements in New South Wales (NSW), Australia. Methods: Individuals with an MM diagnosis in the NSW Cancer Registry between 1985 and 2015 with vital follow-up to 2020, were categorized into 3 previously defined treatment eras according to their diagnosis date (1985–1995, chemotherapy only; 1996–2007, autologous stem cell transplantation; and 2008–2015, novel agents including proteasome inhibitors and immunomodulatory drugs). Both relative survival and cause-specific survival according to Fine and Gray’s competing risks cumulative incidence function were calculated by treatment era and age at diagnosis. Results: Overall, 11,591 individuals were included in the study, with a median age of 70 years at diagnosis. Five-year relative survival improved over the 36-year (1985–2020) study period (31.0% in 1985–1995; 41.9% in 1996–2007; and 56.1% in 2008–2015). For individuals diagnosed before 70 years of age, the 5-year relative survival nearly doubled, from 36.5% in 1985–1995 to 68.5% in 2008–2015. Improvements for those > 70 years of age were less pronounced between 1985–1995 and 1996–2007; however, significant improvements were observed for those diagnosed in 2008–2015. Similar overall and age-specific patterns were observed for cause-specific survival. After adjustment for gender and age at diagnosis, treatment era was strongly associated with both relative and cause-specific survival (P < 0.0001). Conclusions: Survival of individuals with MM is improving in Australia with treatment advances. However, older age groups continue to experience poor survival outcomes with only modest improvements over time. Given the increasing prevalence of MM in Australia, the effects of MM treatment on quality of life, particularly in older age, warrant further attention.
Assessment of parameters of cytokine secretion and proliferative activity in mononuclear blood cell cultures of patients with tick-borne infections
Authors: Ilyinskikh E.N. ,   Siberian State Medical University, Tomsk, Russian Federation, Voronkova O.V. , Samoylov K.V. , Reshetova A.V. , Hasanova R.R. , Petrova E.I. , Esimova I.E. ,   Siberian State Medical University, Tomsk, Russian Federation,   Siberian State Medical University, Tomsk, Russian Federation,   Siberian State Medical University, Tomsk, Russian Federation,   Siberian State Medical University, Tomsk, Russian Federation,   Siberian State Medical University, Tomsk, Russian Federation,   Siberian State Medical University, Tomsk, Russian Federation
Published: July 2024

Associations between the immune response and the severity of tick-borne infections are poorly understood. Objective. Assessment of the parameters of cytokines secretion, proliferative activity and cytogenetic instability in the mononuclear blood cell cultures of patients with tick-borne encephalitis febrile form and ixodid tick-borne borreliosis nonerythemal form at the onset of the disease. Patients and methods. We assessed 24 parameters in the phytohemagglutinin-stimulated mononuclear blood cell cultures from 25 patients with tick-borne encephalitis, 24 patients with ixodid tick-borne borreliosis, and from 14 healthy donors. Statistical analysis included the Mann–Whitney test, Pearson’s goodness-of-fit test, Spearman’s rank correlation, and ROC analysis. Results. The patients compared with the controls showed a suppression of the stimulated blast transformation, increases in the levels of the micronucleated T-lymphocytes and the secretion of not only pro-inflammatory cytokines – tumor necrosis factor (TNF)-α and interleukin (IL)-6, but IL-10 and IL-4 as well. Pyrexia levels were negatively correlated with the blast transformation and positively correlated with the micronucleated T-cells levels and pro-inflammatory cytokines secretion. The predictors for the diagnosis of tick-borne encephalitis compared with borreliosis were decreases in the blank and/or stimulated levels of blast transformation and the production of TNF-α, IL-6, interferon-γ as well as increases in the micronucleated T-cell levels and the secretion of IL-8 and IL-10. Conclusion. The results represent the features of the immune response reactions and its association with the severity of the clinical picture. Key words: blast transformation, ixodid tick-borne borreliosis, tick-borne encephalitis, micronucleus assay, mononuclear blood cells, phytohemagglutinin, cytokines
Validasi Metode Analisis Fosfor pada Sampel Daging dengan Pereduksi Hidrazin Sulfat menggunakan Spektrofotometri Uv-Vis
Authors: Khanza Adelia Nisrina , Pratiwi Yussi
Published: July 2024

Abstrak
Pakan ternak yang biasanya dikonsumsi untuk menjaga nutrisi ternak mengandung fosfor. Fosfor dalam pakan ternak dapat menyebabkan akumulasi fosfor dalam daging ternak. Oleh karena itu, analisis fosfor secara berkala diperlukan untuk memastikan makanan tersebut aman untuk dikonsumsi. Analisis fosfor diukur dengan menggunakan metode spektrofotometri UV-Vis dengan menentukan kondisi optimum untuk analisis fosfor kemudian dilanjutkan validasi metode analisis yang terdiri dari parameter linearitas, sensitivitas, batas deteksi dan kuantitasi, akurasi dan presisi. Reaksi molibdenum biru menghasilkan senyawa kompleks fosfomolibdat dengan panjang gelombang 689 nm. Validasi metode analisis fosfat tereduksi hidrazin sulfat memiliki linearitas dengan nilai R2 = 0,9984 pada rentang 0 - 0,05 mg L-1 . Absorptivitas molar sebesar 7,9259 x 104 L mol-1 cm-1 . Nilai limit deteksi (LOD) sebesar 5,26 x 10-4 mg L-1 dan nilai limit kuantitasi (LOQ) sebesar 1,59 x 10-3 mg L-1 . Nilai presisi dinyatakan dalam persen simpangan baku relatif %RSD pada daging bebek, ayam dan sapi sebesar 1,33%, 1,34% dan 1,08%. Persen perolehan kembali yang diperoleh untuk setiap sampel daging berkisar antara 90% - 117%. Fosfat yang terkandung dalam daging bebek, ayam dan sapi tidak melebihi baku mutu menurut Persatuan Ahli Gizi Indonesia (PERSAGI).
Kata kunci: hidrazin sulfat, metode analisis fosfor, spektrofotomateri UV-Vis
Abstract
Animal feed which is usually consumed to maintain the nutrition of livestock contains phosphor. The phosphor in animal feed may cause an accumulated phosphor in meat. Therefore, a regular phosphor analysis is needed to make sure the food is safe. Phosphor analysis is measured by using spectrophotometry UV-Vis method by determining the optimum conditions for phosphorus analysis and then continued analysis validation which consists of linearity parameter, sensitivity, limit of detection and quantitation, accuracy, and precision. The reaction of blue molybdenum results in complex phosphomolybdate compound with a wavelength of 689 nm. The method validation of analysis of phosphate reduced hydrazine sulfate have a linearity of the method with R2=0.9984 in the range of 0 - 0.05 mg L-1 . The molar absorptivity of 7.9259 x 104 L mol-1 cm-1 . The value of limit of detection (LOD) of 5.26 x 10-4 mg L-1 and quantitation limit (LOQ) value of 1.59 x 10-3 mg L-1 . The precision values were expressed as percent of the relative standard deviations of %RSD in duck, chicken and cow of 1.33%, 1.34% and 1.08%. The percent recovery is obtained for each sample of meat ranged from 90% - 117%. Phosphate contained in duck, chicken and cow did not exceed the quality standard according to the Indonesian Nutritionist Association (PERSAGI).
Keywords: analysis fosfor method, hidrazin sulfat, spektrofotomateri UV-Vis
Validasi Metode Analisis Kandungan Nitrogen Dioksida di Lingkungan Kerja menggunakan Metode Griess-Saltzman
Authors: Al Faruq Fauzan Mujahid , Purnomo Dwi
Published: July 2024

Abstrak
Nitrogen dioksida merupakan adalah gas beracun yang dihasilkan terutama sebagai produk sampingan dari proses pembakaran seperti mesin mobil, pembangkit listrik, dan asap rokok. Gas NO2 dapat menyebabkan berbagai penyakit pernafasan akut. Karne NO2  termasuk polutan berbahaya, maka dibutuhkan pemantauan pada NO2 untuk membuat lingkungan lebih aman dan bersih.  Dalam pemantauan konsentrasi NO2 di udara ambien terdapat suatu metode yang telah ditetapkan dalam SNI:7119-2:2017. Metode tersebut diperlukan diuji kembali agar sesuai dengan ketentuan dalam pengujian kadar NO2 di lingkungan kerja yang memiliki nilai ambang batas yang berbeda dengan udara ambien. Untuk mencapai hal tersebut harus dilakukan validasi metode sebagai pemenuhan syarat bahwa metode dapat diaplikasikan dalam analisa. Validasi metode tersebut dilakukan penentuan linearitas, batas deteksi dan batas kuantifikasi, uji presisi dan akurasi metode, ketahanan dan ketangguhan metode uji, dan estimasi ketidakpastian pengukuran. Hasil menunjukkan bahwa dari seluruh parameter uji telah memenuhi syarat keberterimaan.
Kata kunci: lingkungan kerja, metode griess-saltzman, nitrogen dioksida
Abstract
Nitrogen dioxide is a toxic gas produced mainly as a by-product of combustion processes such as automobile engines, power plants, and cigarette smoke. NO2 gas can cause various acute respiratory diseases. Since NO2 is a hazardous pollutant, it is necessary to monitor NO2 to make the environment safer and cleaner.  In monitoring the concentration of NO2 in ambient air, there is a method that has been established in SNI: 7119-2: 2017. The method needs to be tested again so that it is in accordance with the provisions in testing NO2 levels in the work environment which has a different threshold value from ambient air. To achieve this, method validation must be carried out as a fulfillment of the requirement that the method can be applied in analysis. The validation of the method is carried out by determining linearity, detection limit and limit of quantification, precision and accuracy test of the method, durability and robustness of the test method, and estimation of measurement uncertainty. The results show that all test parameters have met the acceptance requirements.
Keywords: griess-saltzman method, nitrogen dioxide, work environment
Ferâiznâme Literatürüne Zeyl: Hâzık’ın Ferâ’izü’l-Manzûme’si
Authors: Gözitok Mehmet Akif , Çavuşoğlu Halime
Published: July 2024

Fıkıh ilminin en önemli konularından kabul edilen ferâiz ilmi, en genel tanımıyla ölen bir kişinin ardında bıraktığı mirasa kimlerin mirasçı olabileceğini ve mirasçıların alacakları payların nasıl hesaplanacağını açıklayan bir ilim dalıdır. Türk-İslam edebiyatının dinî muhtevalı edebî türlerinden kabul edilen ferâiznâmeler, bu ilme dair yazılmış manzum ve mensur eserlerdir. Anadolu sahası Türk edebiyatında ilk örnekleri XV. yüzyılda görülen ferâiznâme türünün, XX. yüzyıla kadar devam ettiği ve irili ufaklı birçok örneğinin kaleme alındığı bilinmektedir. Bu edebî türün kayıp halkalarından biri de XVIII. yüzyılın önde gelen âlim ve fazıl şairlerinden kabul edilen Erzurumlu Muhammed Hâzık Efendi’nin Ferâ’izü’l-Manzûme adlı eseridir. Biyografik ve bibliyografik kaynaklarda hakkında hiçbir bilgi bulunmayan söz konusu bu eserde Hâzık, talebelerinin ferâiz ilmini daha kolay öğrenebilmesi ve elzem kısımlarını ezberleyebilmesi için ferâiz ilminin önemli metinlerini muhtasar olarak nazmen ele almıştır. Bu çalışma, bilinen tek nüshası Atatürk Üniversitesi Merkez Kütüphanesi Seyfettin Özege Bölümü Satın Alınanlar Koleksiyonu 35 numarada kayıtlı olan Ferâ’izü’l-Manzûme’nin ilmî neşri ile dil, üslup, muhteva ve şekil bakımından incelenmesini amaçlamaktadır.
Mixed, hybrid training as a necessary component of modern training of medical professionals.
Authors: Sviridova T.B. ,   Branch of the Kirov Military Medical Academy of the Ministry of Defense of the Russian Federation in Moscow, Makiev R.G. , Lutikov A.S. , Goldina E.A. ,   Deputy Head of the Academy for Academic Affairs, S. M. Kirov Military Medical Academy of the Ministry of Defense of the Russian Federation,   GBUZ «Diagnostic Clinical Center No. 1 DZM»,   Branch of the Kirov Military Medical Academy of the Ministry of Defense of the Russian Federation in Moscow
Published: July 2024

The article presents a review of the literature on the introduction of mixed, hybrid training in educational programs of additional professional education of medical workers. Definitions of mixed and hybrid learning are given. Publications, research and best practices related to mixed, hybrid training of medical professionals are considered. Attention is focused on the importance of this approach and information is provided that can be useful for teachers, educational institutions and medical professionals seeking to update their skills and knowledge.
Osseous Triceps Avulsion in a 12-Year-Old Girl
Authors: Hernandez Sean G. , McQueen Ryan G. , Erickson John B.
Published: July 2024

Case:

A 12-year-old girl presented with significant right elbow pain following a fall during soccer which caused an osseous triceps avulsion injury and nondisplaced type II Salter-Harris radial neck fracture. The patient was treated with successful open repair utilizing suture anchor fixation, resulting in full return of function and return to previous activities.

Conclusion:

Timely and accurate diagnosis and treatment of displaced triceps sleeve avulsion injuries is critical and can result in excellent patient outcomes and return to previous functional level. This unique case contributes to the diagnosis and management of this rare condition in pediatric populations.
Visualization of the Values of the AL Anwar Islamic Boarding School to Improve the Personal Qualities of Santri
Authors: Kurli Anis , Hidayat Hidayat , Hidayat Imam , Zarnuji Zarnuji , Norrahman Rico
Published: July 2024

The Industrial Revolution has changed the values of Islamic Boarding Schools which have begun to fade among students as the nation's next generation. Santri are more busy with their social media than living socially with the community in teaching and exemplifying the characteristic educational values they receive at Islamic boarding schools. In fact, the values of Islamic boarding school education have not been a reflection of improving the quality of students in society. The theory used in this research to instill Islamic boarding school values in students is visualization through documentary videos as a learning medium, so that students can easily access and remember them in everyday life. The results of this research show that there is a change in the behavior of santri in an effort to improve personal quality, namely that the santri are more motivated to learn and are happy to do good as a reflection of the santri as role models for their community. The improvement in personal quality in question is related to increasing the moral quality and knowledge of students. The true philosophy of santri is the continuation of the struggle of teachers and ulama' to guide society in religious knowledge and face increasingly competitive life
Divergent pathogenic dynamics of immature tick-borne and mosquito-borne flaviviruses: a paradigm shift in prM-containing particle infectivity
Authors: Ruzek Daniel , Holoubek Jiri , Salat Jiri , Matkovic Milos , Bednar Petr , Novotny Pavel , Hradilek Martin , Majerova Tatana , Rosendal Ebba , Eyer Ludek , Fortova Andrea , Duskova Michaela , Sakyi Lesley Bell- , Överby Anna , Cavalli Andrea
Published: July 2024

Maturing flaviviruses undergo pH-dependent conformational changes, transitioning from trimeric pre-membrane protein prM and envelope protein E (prM-E) complexes to dimeric E with cleaved prM. However, little is known about the intermediate maturation stages and whether immature virions contribute to infection. Here we demonstrated that immature prM-containing particles of tick-borne flaviviruses—tick-borne encephalitis virus (TBEV), Langat virus, and louping ill virus—exhibited infectivity in mammalian cells under physiological conditions. This finding challenges the long-standing assumption that prM-containing flavivirus particles are non-infectious, as is the case for immature mosquito-borne flaviviruses, including Usutu virus, Zika virus, and West Nile virus (WNV). In a mouse model, prM-TBEV exhibited high pathogenicity, contrasting with the reduced pathogenicity of prM-WNV. Structural modelling, functional validation, and site-directed mutagenesis revealed interactions within the prM-E complex that influence pH sensitivity, furin cleavage site accessibility, and virus infectivity. This study, therefore, elucidates the distinctive dynamics of infectivity of tick- and mosquito-borne flaviviruses.
CD38+ Alveolar macrophages mediate early control of M. tuberculosis proliferation in the lung
Authors: Russell David , Pisu Davide , Mattila Joshua , Johnston Luana
Published: July 2024

Tuberculosis, caused by M.tuberculosis (Mtb), remains an enduring global health challenge, especially given the limited efficacy of current therapeutic interventions. Much of existing research has focused on immune failure as a driver of tuberculosis. However, the crucial role of host macrophage biology in controlling the disease remains underappreciated. While we have gained deeper insights into how alveolar macrophages (AMs) interact with Mtb, the precise AM subsets that mediate protection and potentially prevent tuberculosis progression have yet to be identified. In this study, we employed multi-modal scRNA-seq analyses to evaluate the functional roles of diverse macrophage subpopulations across different infection timepoints, allowing us to delineate the dynamic landscape of controller and permissive AM populations during the course of infection.
Our analyses at specific time-intervals post-Mtb challenge revealed macrophage populations transitioning between distinct anti- and pro-inflammatory states. Notably, early in Mtb infection, CD38- AMs showed a muted response. As infection progressed, we observed a phenotypic shift in AMs, with CD38+ monocyte-derived AMs (moAMs) and a subset of tissue-resident AMs (TR-AMs) emerging as significant controllers of bacterial growth. Furthermore, scATAC-seq analysis of naïve lungs demonstrated that CD38+ TR-AMs possessed a distinct chromatin signature prior to infection, indicative of epigenetic priming and predisposition to a pro-inflammatory response. BCG intranasal immunization increased the numbers of CD38+ macrophages, substantially enhancing their capability to restrict Mtb growth.
Collectively, our findings emphasize the pivotal, dynamic roles of different macrophage subsets in TB infection and reveal rational pathways for the development of improved vaccines and immunotherapeutic strategies.
Hemodynamic Effect of Handgrip During Cyclic Exercise: Study Protocol for a Randomized Crossover Clinical Trial
Authors: Sacramento Marvyn de Santana do , Leite Josias Melo , de Oliveira Alice Miranda , Barbosa Ramon Martins , Souza Pedro Elias Santos , Petto Jefferson
Published: July 2024

Objective: To test the hypothesis that isometric contraction with the Handgrip device modulates the response of heart rate, systolic and diastolic blood pressure, double product, and subjective perception of exertion (Borg) during cyclic exercise.
Method: This randomized crossover clinical trial consists of three intervention arms. Forty male volunteers aged between 20 and 30 will be recruited. After screening, the volunteers will be randomized to treatment protocols (I: without HG; II: with HG at 30% of Handgrip Strength - HGS and III: with HG at 50% of HGS) which involve performing cyclic exercise on a treadmill, with four sprints at moderate intensity. The data will be presented by the variation (∆) of the sprint-rest and post-exercise-rest moments. The one-way Analysis of Variance (ANOVA) test will be performed in the case of symmetrical distribution and Kruskal-Wallys for non-parametric distribution, to analyze the three protocols during the same phases and then the same test to evaluate the individual responses over time. A p≤0.05 will be considered statistically significant.
Impact of the project: This study will provide answers to the hemodynamic effects of isometric activity with handgrip associated with cyclic exercise and, if the hypotheses of this study are confirmed, we will have robust mechanistic evidence to broaden the field of scientific exploration in different clinical contexts.
Clinical trial registration:
Brazilian Registry of Clinical Trials (REBEC): RBR-78fhyrf, registered on November 17, 2023.
Funding: Postgraduate Support Program for Private IES (PROSUP - CAPES) of the Bahiana School of Medicine and Public Health.
Navigating the Future of Learning: A Systematic Review of AI-Driven Intelligent Tutoring Systems (ITS) in K-12 Education
Authors: Charland Patrick , Létourneau Angélique , Martineau Marion Deslandes , Boasen Jared , Léger Pierre-Majorique
Published: July 2024

The use of artificial intelligence in education (AIEd) has grown exponentially in the last decade, particularly using intelligent tutoring systems (ITSs). Despite the increased use of ITSs and their promise to improve learning, their real educational value remains unclear. Therefore, this systematic review aims to identify which experimental designs are currently used to evaluate the effects of ITSs and what are the effects of ITSs on K-12 students' learning and performance. Studies retrieved from ERIC USDE and Scopus databases reporting effects of an ITS in a formal learning setting published after 2009 were screened and the data extracted was categorized by experimental design. The 20 studies analyzed in this systematic review included a total of 2853 students (N = 2853), predominantly in middle and high school Science, Technology, Engineering and Mathematics (STEM) -related classes. These studies used quasi-experimental designs with varying intervention durations, with half of them categorized as very short. Some studies reported positive effects of ITSs on learning and performance in contrast to traditional teaching. However, the effects were found to be mitigated when compared to non-intelligent tutoring systems. The included studies have identified optimal conditions or contexts for the application of specific ITSs. Overall, our findings suggest that the effects of ITSs on learning and performance in K-12 education are generally positive. However, additional research with longer interventions and increased sample sizes with greater diversity is warranted. Additionally, the ethical implications of using AI for teaching should be investigated.
Multigram-scale Synthesis of Volasertib, an Inhibitor of Polo-like Kinases in Clinical Evaluation
Authors: Wang Kang , Zhao Dong , Jin Mingli , Li Yuan , Sun Lei , Zhu Yanli , Wang Chen , Li Shuang , Wang Yu , Miao Qianying , Chen Xiao , Zhao Yanfang , Hou Yunlei
Published: July 2024

This paper described the development of a practical, improved and efficient method for the multigram-scale synthesis of Volasertib, an injectable bioavailable potent and selective inhibitor of PLK1. The key to this optimization was the design and development of a novel synthetic strategy, which involved the preparation of key intermediate 4-amino-N-{4-[4-(cyclopropylmethyl)piperazin-1-yl]cyclohexyl}-3-methoxybenzamide (W-5) through nitro reduction sequence and (7R)-2-Chloro-7-ethyl-7,8-dihydro-8-(1-methylethyl)-6(5H)-pteridinone (W-11) through reductive cyclization and N-methylation reaction. The developed process provided 46.1% overall yield, which enabled us to rapidly synthesize multi-gram quantities of Volasertib in 99.42% purity.
RETRACTED: Seamounts control subducted carbonate recycling in Central America – evidence from stable Sr isotopes
Authors: Hammerstrom Alexander , Parai Rita , Carlson Richard , Turner Stephen
Published: July 2024

The authors have requested the removal of their preprint from Research Square. The author's request does not imply errors or misconduct.
TARGET Based m 6 A Methylation-Related Genes Predict Prognosis Relapsed B-cell Acute Lymphoblastic Leukemia
Authors: Qiu Kun-yin , Liao Xiong-yu , Fang Jian-pei , Zhou Dun-hua
Published: July 2024

Purpose
The current study aim to investigate the significance of N6-methyladenosine (m6A) methylationrelated genes in the clinical prognosis of childhood relapsed B-cell acute lymphoblastic leukemia patient.
Methods
Transcriptome data and corresponding clinical data on m6A methylation-related genes (including 20 genes) were obtained from the Therapeutically Applicable Research To Generate Effective Treatments (TARGET) database.
Results
The bone marrow (BM) samples of 134 newly diagnosed (naive) and 116 relapsed B-ALL from TARGET were enrolled in the current study. Three genes (FTO, HNRNPC, RBM15B) showed significant up-regulation in relapsed B-ALL compared with that in naive B-ALL.The three genes had a significantly worse survival (P < 0.05). The LASSO Cox regression model was used to select the most predictive genes as prognostic indicators, and YTHDC1 and FTO were identified as prognostic factors for relapsed ALL. Finally, the results of multivariate regression analysis showed that the risk score of m6A methylation-related genes was an independent prognostic factor in relapsed B-ALL (P < 0.05).
Conclusion
We found that the expression levels of m6A methylation-related genes were different in naive and relapsed patients with B-ALL and correlated with survival and prognosis.This implies that m6A methylation-related genes may be promising prognostic indicators or therapeutic targets for relapsed B-ALL.
ATF3 is required for the prevention of cardiomyopathy via the regulation of mitochondrial oxidative stress
Authors: Kim Won-Ho , Jeong Myong-Ho , Jeong Yideul , Cho Su-Yeon , Lee Seung Hee , Kim Geun-Young , Kim Min-Ju
Published: July 2024

Activating transcription factor 3 (ATF3) is a critical regulator for cellular stress responses and is implicated in stress-induced cardiac hypertrophy and fibrosis. However, the role of ATF3 in cardiomyopathy remains controversial. Here, we demonstrate that ATF3 plays a cardioprotective role by controlling mitochondrial oxidative stress in angiotensin II (Ang II)-triggered cardiomyopathy. The expression of ATF3 was significantly upregulated in hypertrophic hearts chronically infused with Ang II, which correlated with Ang II-treated cardiomyocytes. In neonatal mouse ventricular myocytes (NMVMs), Ang II-elicited hypertrophic responses were either aggravated or suppressed by ATF3 depletion or overexpression, respectively. Similar results were also obtained in human embryonic stem cell-derived cardiomyocytes (hESC-CMs). To analyze the direct role of ATF3 in cardiomyopathy, we generated mice with a cardiomyocyte-specific ATF3 deletion using a tamoxifen-inducible Cre-recombinase (αMHC-MerCreMer/loxP) system. In response to Ang II infusion, mice with cardiomyocyte-specific ablation of ATF3 (ATF3 cKO) exhibited aggravated cardiac hypertrophy and fibrosis concurrent with decreased fractional shortening and ejection fraction. In addition, the transcriptome analysis of control and cKO hearts revealed alterations in genes related to mitochondrial function and organization. In particular, the expression of Sirt3/Sod2 transcripts, well known as a mechanism for regulating mitochondrial oxidative stress, was increased in Ang II-infused mice, which was downregulated by the depletion of ATF3, suggesting the cardioprotective function of ATF3 through the improvement of mitochondrial function. These results suggest that ATF3 may be a potential therapeutic target for hypertrophic cardiomyopathy.
The relationship between Happiness with Sleep quality and Self-care ability in community-dwelling older adults
Authors: Bakhtiyary Milad , Sharifi Farshad , Karimi Keyvan , Salehpoor-emran Mohammad , Mirzadeh Fatemeh Sadat , Afshar Pouya Farokhnezhad
Published: July 2024

Background
Population aging is one of the challenges of the future, which requires large-scale planning. Examining happiness and its relationship with the quality of sleep and self-care is an aspect that has received less attention in old age. Therefore, this study aimed to determine the relationship between happiness with sleep quality and self-care ability in community-dwelling older adults.
Methods
This is a cross-sectional correlation descriptive research. We selected 306 eligible community-dwelling older adults by multistage sampling referring to comprehensive health service centers. The Oxford Happiness Inventory (OHI), Pittsburgh Sleep Quality Questionnaire (PSQI), and The Self-care Ability Scale for the Elderly (SASE) were used to collect data. Data were analyzed using SPSS v.27 software by Spearman test, univariate and multivariate regression.
Results
The findings showed that 58% of older adults were male, 67.6% were married, and 44% were retired. Multivariate logistic regression showed a significant relationship between self-care ability (β = -1.50, p < 0.001) and sleep quality (β = 0.50, p < 0.001) with happiness.
Conclusion
The level of happiness is directly affected by the self-care ability and sleep quality. Therefore, policymakers and planners should pay more attention to the importance of happiness and its relationship to sleep and self-care ability.
Peritoneal Tumor Dna in a Multimodal Treatment Setting of Locally Advanced Gastric Cancer – A Novel Study Design
Authors: Jacob Sven , Heiliger Christian , Balonov Ilja , Jarmusch Stefanie , Albertsmeier Markus , Holdenrieder Stefan , Oberhofer Angela , Mayer Zsuzsanna , Heinrich Kathrin , Westphalen C. Benedikt , Bösch Florian , Wildgruber Moritz , Lauseker Michael , Xu Yu Jun , Neumann Jens , Lorenzen Sylvie , Renz Bernhard , Stein Hubert , Werner Jens , Angele Martin
Published: July 2024

Background
Gastric cancer is a leading cause of death from malignant diseases and has been characterized on a molecular level in recent years. In a context of precision medicine, the proposed study aims to describe the three major compartments harboring tumor cells in patients requiring multimodal therapy: peripheral blood, the abdominal cavity, and the local tumor. Unlike tumor tissues and liquid biopsies, the peritoneal space (in terms of ascites and metastatic tumor seeds) is not regularly considered in molecular diagnostics due to difficult access. This study aims to determine circulating tumor DNA in the peritoneum (ptDNA) and in the plasma (ctDNA). It will then be investigated to what extent the detection of ptDNA before and after neoadjuvant therapy correlates with ctDNA. The main goal is to evaluate the relative changes in ptDNA and ctDNA levels after systemic treatment in patients with locally advanced gastric cancer.
Hypothesis:
We hypothesize that ptDNA in the abdominal cavity reacts differently to chemotherapy than ctDNA in peripheral blood and therefore, peritoneal disease cannot be adequately represented by ctDNA. Patients with elevated initial ptDNA levels, especially those who do not respond to neoadjuvant therapy, might have an increased risk of peritoneal carcinomatosis and consequently require intensified systemic or local (i.e. HIPEC) treatments.
Study design:
ptDNA and ctDNA will be analyzed at the time of cancer diagnosis (during exploratory laparoscopy according to diagnostics guidelines) and after neoadjuvant therapy (during tumor resection). Subsequently, the determination of ptDNA in gastric cancer patients could allow the identification of a subgroup that will benefit from intensified local treatment options for peritoneal tumor seeding. Finally, the study will attempt to establish the peritoneal space as another compartment for future precision oncology diagnostics and multimodality therapy.
Pericardioperitoneal window creation for recurrent pericardial tamponade: A case report and literature review
Authors: EKİM Hasan , EKİM Meral
Published: July 2024

Background: As a result of the increased life expectancy of cancer patients due to developing effective treatments applied, the incidence of secondary patterns such as malignant pericardial effusion has increased. Therefore, pericardial effusions leading to cardiac tamponade have been becoming an increasingly common emergency in medical practice. Although positive results are obtained with echocardiography-guided pericardiocentesis or percutaneous catheter drainage in pericardial effusions causing tamponade, the effusion may recur in some malignant cases. In the management of such patients, it is desirable to provide permanent drainage by opening a pericardial window instead of performing frequent pericardiocentesis.
Case Presentation: A 62-year-old male patient with tamponade was admitted to our hospital. The clinical picture was corrected by pericardiocentesis accompanied by echocardiography. However, due to rapid fluid accumulation that required pericardiocentesis every other day, he was referred to the cardiovascular surgery department for surgical drainage. The patient clinically improved after the subxiphoid pericardial window was created. However, when symptoms of tamponade developed again 24 hours after the chest tube was removed, a pericardioperitoneal window was opened with a subxiphoidal approach. There were no recurrences of effusion for three months until the patient died of malignancy.
Conclusion: In patients who have undergone pleurodesis or who are not suitable for general anesthesia due to their hemodynamic conditions, the best surgical procedure to prevent recurrent effusions causing tamponade is to create a pericardioperitoneal window under local anesthesia.
Experimental study on the fracture morphology of chips in 50 steel internal chip removal deep hole drilling
Authors: He Qingqiang , Sun fazhe , Zhang Shuai , Zou Zhijun , Pang Kun , Che Hang , Zhang Lijun
Published: July 2024

Relying solely on experience and trial cutting to determine the range of main process parameters for deep hole machining in order to control the cutting shape is time-consuming, laborious, and costly. This article simplifies chips into three-dimensional curved beams with rectangular cross-sections, and theoretically analyzes the stress states at different stages of their formation process, clarifying the mechanical causes of the three-dimensional shape of chips; A self-developed internal chip removal deep hole drilling device was used to drill 50 steel φ 30 mm deep hole machining experiment determined that the values of chip breakage rate and compression rate for small diameter deep hole drilling were less than 10 by calculating the chip breakage rate and compression rate of each tooth. This is a key quantitative indicator for controlling chip shape to achieve good chip breakage and removal; By using scanning electron microscopy, the microscopic morphology characteristics of the chip fracture surfaces of each tooth under different process parameter combinations were analyzed in detail, and the effects of feed rate and speed on chip morphology were explored, providing data reference for the rational selection of process parameters for small diameter inner chip removal deep hole machining.
Optimizing Heterologous Production of CRISPR-AsCas12a Protein in Escherichia coli
Authors: Goméz-Quintero Orlando S. , Morales-Moreno Melissa D. , Valdés-Galindo Erick G. , Cárdenas-Guerra Rosa Elena , Hernández-García Armando
Published: July 2024

The CRISPR-Cas12a system is a groundbreaking tool that has seen an ample use for genome editing and diagnostics in biotechnology and biomedicine research labs. Despite its increasing use, there is a lack of studies on optimizing Cas12a protein production at lab-scale using straightforward protocols. This study aimed on enhancing the lab-scale recombinant production of Acidaminococcus sp Cas12a protein (AsCas12a) in E. coli. Through careful adjustments of simple parameters, the production of AsCas12a was remarkably increased. Optimized conditions involved using the BL21(DE3) strain, TB medium with 1% glucose, induction with 0.3 mM IPTG for at least 6–9 h and incubation at 30°C. Notably, these conditions deviate from conventional production protocols for Cas12a and related proteins such as Cas9 from Streptococcus pyogenes. Upon combination of all optimized conditions bacterial production of AsCas12a improved ~ 3 times, passing from 0.95 mg / mL of bacterial lysate volume, for non-optimized conditions, to 3.73 mg/mL in the optimal ones. The production yield of AsCas12a protein, after chromatographical purification increased ~ 4.5 times, from 5.2 to 23.4 mg/L (culture volume) without compromising its functionality at all. The purified AsCas12a protein retained full activity for programmable in vitro DNA cis-cleavage and for collateral trans-activity, which was used to detect the N gene from SARS-CoV-2. This optimized method offers an efficient and high-yield AsCas12a protein production using materials and conditions that are accessible to many research labs around the world.
The effect of incubator humidity on morbidity and mortality in preterm infants: A systematic review
Authors: Chen Zhiqin , Lin Ruizi , Wang Huixin , Shi Bijun , Chen Qian
Published: July 2024

Purpose
To assess the association between different incubator humidity levels and clinical outcomes in preterm infants.
Background
Since there is no well-accepted standard for delivery of incubator humidity for preterm infants. A meta-analysis is needed to summarize status of current research.
Methods
Searched 5 databases, including PubMed, the Cochrane Library, Embase, Ovid, and Web of Science, published between January 2000 and December 2023. Randomized control trials, prospective cohort studies and retrospective cohort studies were included if they assessed how different incubator humidity levels affected preterm infants with a gestational age < 34 weeks, published in English. Infection rates, the incidence of bronchopulmonary dysplasia and predischarge mortality were evaluated.
Results
Included in this review were 3 randomized control trials and 3 cohort studies including 801 preterm infants. Findings revealed that a high humidity level increased the incidence of infection in preterm infants (RR = 1.26, 95% CI 1.02, 1.55, P = 0.03). No significant difference was found between a high humidity incubator humidity levels and the incidence of bronchopulmonary dysplasia or infant mortality.
Conclusions
This study found that high humidity levels had a significant impact on the incidence of infection.
Implications for Practice and Research
: Evidence from the two decades showed that high humidity levels significantly increased the incidence of infection in preterm infants. When considering a humidity delivery plan, the impact of high humidity levels on the incidence of infection should be carefully considered. High-quality, large-sample and multicenter trials are needed for further validation.
Insulin-like growth factor binding protein-3 in oral potentially malignant disorders and oral squamous cell carcinoma: A Systematic Review
Authors: Ahire Manisha S. , Nagar Saurabh R. , Ivy Zaneta , Tupkari J. V. , Dalvi Shubhangi M. , Mehta Vini
Published: July 2024

Background: To critically review published literature on change in serum value and immunohistochemical (IHC) expression of Insulin growth factor binding proteins (IGFBPs) in oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma. (OSCC)
Methods: Online electronic databases such as PubMed-Medline, Embase, and Scopus were searched using appropriate keywords from the earliest available date till 30th December 2021without restriction on language. Additional sources like Google Scholar, major journals, unpublished studies, conference proceedings, and cross-references were explored. The methodological quality of the studies was evaluated using the “A Cochrane Risk of Bias Assessment Tool: for Non-Randomized Studies of Interventions” (ACROBAT-NRSI).
Results: Six out of 989 titles met the eligibility criteria. Blood (serum/plasma) and biopsied tissue were the two tissue samples used. Total 614 OSCC patients, 105 OPMD patients and 110 cases were included in the control group. A rise in serum IGFBP-3 in stage I OSCC patients were seen. Reduced serum IGFBP-3, which was associated with stage III and IV OSCC cases, was seen in 4 studies. OPMDs showed dual results.
Conclusions: IGFBP-3 plays a role in tumour suppression and promotion. A raised serum value in the initial stages highlights its role in evading malignant transformation, while its raised IHC expression in advancing grades of OSCC reveals its mitogenic potential.
Registration: CRD42021277176
Trial Registration: Not applicable