bluuebunny/crossref_metadata_2025 huggingface dataset:
Water Relation, Gas Exchange Characteristics and Yield Performance of Selected Mungbean Genotypes under Low Soil Moisture Condition
Authors: Tamanna Tahmina , Islam Md. Moshiul , Chaity Arpita Roy , Shams Shahjadi-Nur-Us , Rasel Md. Asadujjaman , Haque M. Moynul , Miah Md. Giashuddin , Alamri Saud , Murata Yoshiyuki
Published: April 2023

Among the environmental constraints, the growth and yield of crops are seriously impaired by moisture stress. With this view, an experiment was conducted to observe genotypic differences in water relation, gas exchange characteristics and yield performance of mungbean under low soil moisture conditions. Experimental variables consisted of five drought tolerant genotypes (G88, G108, G141,varietiesG186), one susceptible genotype (G43) and two standard check variety (BU mug 5, Binnamoog-8) which assigned to two moisture regimes viz., water regime A ((80 to 90% field capacity (FC)) and water regime B (40 to 50% FC). Results showed that water saturation deficit, water uptake capacity and transpiration rate were the lowest in tolerant genotypes G88 followed by genotypes G141, while those were the highest in susceptible genotype G43 under low soil moisture conditions. Contrarily, the highest amount of relative water content and water retention capacity were found in tolerant genotypes G141, G108 and G88 and the lowest was recorded in susceptible genotype G43 under low soil moisture conditions. In the case of the photosynthetic rate and stomatal conductance, the tolerant genotype G141, G88 and G108 showed the higher values at moisture stress condition. The highest total chlorophyll content and proline content were also found in tolerant genotype G88 followed by G141 and G108, and the lowest was found in susceptible genotype G43 under moisture stress conditions. Irrespective of genotypes, moisture stress significantly decreased the yield attributes and yield of mungbean genotypes. However, the highest seed yield per plant (12.11 g) was found in tolerant genotype G88 under low soil moisture conditions because of its lowest reduction rate of yield attributes under moisture stress. Similar responses were also observed in tolerant genotypes G141 and G108. Therefore, the genotypes G88, G108 and G141 showed better performance in the case of water relation and gas exchange characteristics which might be contribute to higher yield of those genotypes.
Effects of Nitrogen Fertilization and Soil Water Content on Seed and Oil Yield in Perennial Castor in a Mediterranean Environment
Authors: Calcagno Silvio , Piccitto Alessandra , Patanè Cristina , Cosentino Salvatore L. , Testa Giorgio
Published: April 2023

Castor (Ricinus communis L.) is an oilseed species that can be grown as a semi-perennial in Mediterranean environments, including the coastal areas of Sicily. The present study investigated the optimization of cultivation techniques for castor, with the crop being maintained over a two-year period, through the evaluation of different agronomical inputs in order to increase seed yield. The effects of irrigation (I) and nitrogen fertilization (N) on the seed and oil yield and their components were assessed in castor cultivated in a typical semi-arid environment. Four levels of irrigation (I0, I30, I60, and I100: 0, 30, 60, and 100% of crop evapotranspiration—ETm restoration, respectively) as the main plot and three levels of nitrogen fertilization (N) (0, 60, and 120 kg N ha−1) as the sub-plot were considered. Irrigation mostly affected the number of racemes per plant, the number of capsules per raceme, and the seed weight. The oil content was, on average, 39.2% and 45.6% for the first and second year, respectively. The highest seed yield was obtained by I100N120 treatment (4154.0 kg ha−1); however, the combination of a high soil nitrogen level (N120) and medium water availability (I60) resulted in satisfactory seed and oil yields. The reduction in the irrigation water to an intermediate level could be also an environmentally friendly strategy not significantly affecting yields.
Lack of Significant Effects of Glyphosate on Glyphosate-Resistant Maize in Different Field Locations
Authors: Bidóia Vitor Simionato , Neto José Cristimiano dos Santos , Maciel Cleber Daniel de Goes , Tropaldi Leandro , Carbonari Caio Antonio , Duke Stephen Oscar , Carvalho Leonardo Bianco de
Published: April 2023

Glyphosate-resistant (GR) maize is dominant in countries where it is grown. Significant, adverse effects of glyphosate application to GR maize have been reported, but few data from robust studies exist to determine if such effects are common. In this study, the effects of recommended application rates (single and sequential applications) were used on GR maize grown at two locations for one season and for two seasons in a third location. No significant effects of glyphosate on mineral content (N, P, K, Ca, Mg, S, Cu, Fe, Mn, and Zn) in leaves or grain, plant height, stem diameter, ear parameters, or yield were found at any location or in any growing season. Likewise, harvested grain quality, as determined by percent starch, protein, and total lipids, was unaffected by glyphosate treatment at any location. Neither glyphosate nor aminomethylphosphonic acid, the primary degradation product of glyphosate, were found in grain from any treatment at any location, except for 20 ng g−1 of glyphosate found in grain from one season at one location. These results support the view that recommended applications of glyphosate have no significant effects on growth, grain composition, mineral content, grain quality, nor yield of GR maize.
Equine Gastric Ulcer Syndrome: An Update on Current Knowledge
Authors: Vokes Jessica , Lovett Amy , Sykes Benjamin
Published: April 2023

Equine Gastric Ulcer Syndrome (EGUS) is a term that has been used since 1999, initially being used to describe all gastric mucosal disease in horses. Since this time, the identification of two distinct main disease entities of the equine gastric mucosa have been described under the umbrella of EGUS; these are Equine Squamous Gastric Disease (ESGD) and Equine Glandular Gastric Disease (EGGD). In 2015 the European College of Equine Internal Medicine (ECEIM) released a consensus statement defining these disease entities. This document highlighted the lack of evidence surrounding EGGD compared to ESGD, and identified knowledge gaps for further research to be directed. Subsequently, many studies on EGGD have been published, especially on pathophysiology, diagnosis, and treatment. This article updates current knowledge on both ESGD and EGGD as understanding has evolved since the last large-scale review.
Linezolid Pharmacokinetics and Its Association with Adverse Drug Reactions in Patients with Drug-Resistant Pulmonary Tuberculosis
Authors: Padmapriyadarsini Chandrasekaran , Solanki Rajesh , Jeyakumar S. M. , Bhatnagar Anuj , Muthuvijaylaksmi M. , Jeyadeepa Bharathi , Reddy Devarajulu , Shah Prashanth , Sridhar Rathinam , Vohra Vikram , Bhui Namrata Kaur
Published: April 2023

We evaluated the relationship between the pharmacokinetic parameters of linezolid (LZD) and development of adverse drug reactions (ADRs) in patients with pulmonary drug-resistant tuberculosis. A prospective cohort of adults with pulmonary multidrug-resistant tuberculosis with additional resistance to fluoroquinolone (MDR-TBFQ+) received treatment with bedaquiline, delamanid, clofazimine, and LZD. Blood samples were collected during weeks 8 and 16 at eight time points over 24 h. The pharmacokinetic parameters of LZD were measured using high-performance liquid chromatography and associated with ADRs. Of the 165 MDR-TBFQ+ patients on treatment, 78 patients developed LZD-associated anemia and 69 developed peripheral neuropathy. Twenty-three patients underwent intense pharmacokinetic testing. Plasma median trough concentration was 2.08 µg/mL and 3.41 µg/mL, (normal <2 µg/mL) and AUC0-24 was 184.5 µg/h/mL and 240.5 µg/h/mL at weeks 8 and 16, respectively, showing a linear relationship between duration of intake and plasma levels. Nineteen patients showed LZD-associated ADRs-nine at week 8, twelve at week 16, and two at both weeks 8 and 16. Thirteen of the nineteen had high plasma trough and peak concentrations of LZD. A strong association between LZD-associated ADRs and plasma LZD levels was noted. Trough concentration alone or combinations of trough with peak levels are potential targets for therapeutic drug monitoring.
Potential Benefits of Antioxidant Phytochemicals on Endogenous Antioxidants Defences in Chronic Diseases
Authors: Fratta Pasini Anna Maria , Cominacini Luciano
Published: April 2023

Chronic diseases and cancer are worldwide health problems which result in death and disability for millions of people [...]
Antioxidant and Immune Stimulating Effects of Allium cepa Skin in the RAW 264.7 Cells and in the C57BL/6 Mouse Immunosuppressed by Cyclophosphamide
Authors: Kim Ji-Su , Lee Eun-Byeol , Choi Ji-Hye , Jung Jieun , Jeong Un-Yul , Bae Ui-Jin , Jang Hwan-Hee , Park Shin-Young , Cha Youn-Soo , Lee Sung-Hyen
Published: April 2023

Allium cepa L. (onion) has been reported to have various pharmacological effects, such as preventing heart disease, and improving antimicrobial activity and immunological effects. The Republic of Korea produced 1,195,563 tons of onions (2022). The flesh of onion is used as food while the onion skin (OS) is thrown away as an agro-food by-product and is considered to induce environmental pollution. Thus, we hypothesize that increasing usage of OS as functional food material could help protect from the environment pollution. The antioxidant effects and immune-enhancing effects of OS were evaluated as functional activities of OS. In this study, OS showed high 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and xanthine oxidase (XO) inhibitory activity. The antioxidant activities increased in a dose-dependent manner. The IC50 values of DPPH, ABTS radical scavenging activity, and XO inhibitory activity were 954.9 μg/mL, 28.0 μg/mL, and 10.7 μg/mL, respectively. Superoxide dismutase and catalase activities of OS in RAW 264.7 cells were higher than those of the media control. There was no cytotoxicity of OS found in RAW 264.7 cells. Nitric oxide and cytokines (IL-1β, IL-6, IFN-γ, and TNF-α) concentrations in RAW 264.7 cells significantly increased in a dose dependent manner. Immune-stimulating effects of OS were evaluated in immunosuppressed mice induced by cyclophosphamide. White blood cell count and the B cell proliferation of splenocytes were higher in OS100 (OS extract 100 mg/kg body weight) and OS200 (OS extract 200 mg/kg body weight) groups than in the negative control (NC) group. Serum IgG and cytokine (IL-1β and IFN-γ) levels were also higher in OS100 and OS200 groups than in the NC group. OS treatment increased NK cell activity compared with the NC group. The results suggested that OS can improve antioxidant and immune stimulating effects. The use of OS as functional supplement can reduce the agro-food by-product and it may contribute to carbon neutrality.
The Effect of Aromatic Plant Extracts Encapsulated in Alginate on the Bioactivity, Textural Characteristics and Shelf Life of Yogurt
Authors: Popescu Liliana , Cojocari Daniela , Ghendov-Mosanu Aliona , Lung Ildiko , Soran Maria-Loredana , Opriş Ocsana , Kacso Irina , Ciorîţă Alexandra , Balan Greta , Pintea Adela , Sturza Rodica
Published: April 2023

The article investigated the antioxidant and antimicrobial activity of extracts from two aromatic plants—Satureja hortensis L. (SE) and Rosmarinus officinalis L. (RE), encapsulated in alginate, on—yogurt properties. The encapsulation efficiency was controlled by FTIR and SEM analysis. In both extracts, the individual polyphenol content was determined by HPLC–DAD–ESI-MS. The total polyphenol content and the antioxidant activity were spectrophotometrically quantified. The antimicrobial properties of SE and RE against gram-positive bacteria (Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus, Geobacillus stearothermophilus), gram-negative bacteria (Escherichia coli, Acinetobacter baumannii, Salmonella abony) and yeasts (Candida albicans) were analyzed in vitro. The encapsulated extracts were used to prepare the functional concentrated yogurt. It was established that the addition of 0.30–0.45% microencapsulated plant extracts caused the inhibition of the post-fermentation process, the improvement of the textural parameters of the yogurt during storage, thus the shelf life of the yogurt increased by seven days, compared to the yogurt simple. Mutual information analysis was applied to establish the correlation between the concentration of the encapsulated extracts on the sensory, physical-chemical, and textural characteristics of the yogurt.
Denovo-GCN: De Novo Peptide Sequencing by Graph Convolutional Neural Networks
Authors: Wu Ruitao , Zhang Xiang , Wang Runtao , Wang Haipeng
Published: April 2023

The de novo peptide-sequencing method can be used to directly infer the peptide sequence from a tandem mass spectrum. It has the advantage of not relying on protein databases and plays a key role in the determination of the protein sequences of unknown species, monoclonal antibodies, and cancer neoantigens. In this paper, we propose a method based on graph convolutional neural networks and convolutional neural networks, Denovo-GCN, for de novo peptide sequencing. We constructed an undirected graph based on the mass difference between the spectral peaks in a tandem mass spectrum. The features of the nodes on the spectrum graph, which represent the spectral peaks, were the matching information of the peptide sequence and the mass spectrum. Next, the Denovo-GCN used CNN to extract the features of the nodes. The correlation between the nodes was represented by an adjacency matrix, which aggregated the features of neighboring nodes. Denovo-GCN provides a complete end-to-end training and prediction framework to sequence patterns of peptides. Our experiments on various data sets from different species show that Denovo-GCN outperforms DeepNovo with a relative improvement of 13.7–25.5% in terms of the peptide-level recall.
Laurus nobilis Leaves and Fruits: A Review of Metabolite Composition and Interest in Human Health
Authors: Awada Fatme , Hamade Kamar , Kassir Mounir , Hammoud Zeinab , Mesnard François , Rammal Hassan , Fliniaux Ophelie
Published: April 2023

Laurus nobilis belongs to the family Lauraceae and is characterized by its evergreen nature. It is well known because of its traditional use in cooking and folk medicine. Many studies have been performed regarding its chemical composition and the pharmacological potential it exhibits. It has been reported to have antioxidant, antibacterial, antifungal, antiviral, biocidal, antidiabetic, antiulcerogenic, neuroprotective, analgesic, anti-inflammatory, and anticholinergic properties, making this plant of great clinical importance. The main chemical compounds that show bioactive properties in L. nobilis are terpenoids, phenolics, and fatty acids. The purpose of this review is to describe the main use of L. nobilis, notably of the leaves and fruits, and to relate their metabolite content with their pharmacological and toxicological properties.
Investigation of Microseismic Characteristics of Rock Burst Based on Fractal Theory
Authors: Wang Ping , Zhao Ze , Zhang Da , Chen Zeng
Published: April 2023

Microseismic monitoring is a common monitoring tool in the mining production process; for supervising a huge amount of microseismic data, effective analysis tools are necessary. In this study, the monitoring results of microseismic events at the Maoping lead-zinc mine in Yiliang County, Yunnan Province, and the spatiotemporal distribution characteristics of microseismic events are analyzed. We analyze the temporal characteristics of microseismic events using fractal theory, combining the change in fractal dimension with the rock burst incubation process. We also construct an observation area model for event anomalies based on the spatial distribution characteristics of microseismic events. The results show that the growth of the fractal dimension is consistent with the trend of the incubation process before rock burst, and the larger the fractal dimension, the higher the rock burst risk. The observation model, based on the density of microseismic events, can effectively refine the rock burst discrimination range and facilitate subsequent observations. An effective and feasible method of microseismic analysis is provided.
ICT Skills in the Deployment of 21st Century Skills: A (Cognitive) Developmental Perspective through Early Childhood
Authors: Weber Anke Maria , Greiff Samuel
Published: April 2023

ICT technologies are an integral part of today’s digitized society. Therefore, it is important that children acquire ICT skills as part of 21st century skills education to prepare them for later life. Drawing on the literature, seven 21st century skills can profit from the addition of ICT skills, i.e., technical, information, communication, collaboration, critical thinking, creative, and problem-solving skills. While many efforts have been made to integrate ICT skills as part of 21st century skills education into primary and secondary school curricula, the implementation of these skills in early childhood education and care remains a challenge due to developmental concerns. This paper aims to uncover developmental antecedents for ICT 21st century skills in early childhood, mainly addressing children’s cognitive development, and propose ways to implement these skills in child-friendly ways. Drawing on the literature on developmental psychology, seven cognitive developmental antecedents were identified: inductive, deductive, abductive, causal, and scientific reasoning, executive functions, and computational thinking. Moreover, five additional developmental antecedents were identified: fine motor skills, language development, self-regulation, social-emotional development, and creativity. On the backdrop of these antecedents, ways of implementing ICT skills as part of 21st century skills education in early childhood classrooms are proposed that include digital games and learning apps, collaborative play or problem-solving activities with toy robots.
Improving Film Cooling Efficiency with Lobe-Shaped Cooling Holes: An Investigation with Large-Eddy Simulation
Authors: Wang Kefu , Ao Yiqun , Zhao Kai , Zhou Tao , Li Feng
Published: April 2023

Shaped cooling holes have received considerable attention in recent years due to their potential to improve heat transfer while minimizing pressure drop. In this study, the effects of lobe-shaped cooling holes on film cooling performance and turbulent flow characteristics are investigated using large-eddy simulation (LES). The LES approach was validated by comparing the calculated film cooling effectiveness with experimental data. The results show that well-designed lobe-shaped cooling holes can improve the cooling efficiency by up to 37% and reduce the total pressure drop. Additionally, the cooling jets of lobe-shaped cooling holes have a smaller angle to the wall surface, indicating better wall attachment. However, poorly designed lobe-shaped cooling holes can reduce the cooling efficiency. Overall, this study suggests that lobe-shaped cooling holes are a promising technology for improving gas turbine engine performance.
Image-Based Malware Detection Using α-Cuts and Binary Visualisation
Authors: Saridou Betty , Moulas Isidoros , Shiaeles Stavros , Papadopoulos Basil
Published: April 2023

Image conversion of malicious binaries, or binary visualisation, is a relevant approach in the security community. Recently, it has exceeded the role of a single-file malware analysis tool and has become a part of Intrusion Detection Systems (IDSs) thanks to the adoption of Convolutional Neural Networks (CNNs). However, there has been little effort toward image segmentation for the converted images. In this study, we propose a novel method that serves a dual purpose: (a) it enhances colour and pattern segmentation, and (b) it achieves a sparse representation of the images. According to this, we considered the R, G, and B colour values of each pixel as respective fuzzy sets. We then performed α-cuts as a defuzzification method across all pixels of the image, which converted them to sparse matrices of 0s and 1s. Our method was tested on a variety of dataset sizes and evaluated according to the detection rates of hyperparameterised ResNet50 models. Our findings demonstrated that for larger datasets, sparse representations of intelligently coloured binary images can exceed the model performance of unprocessed ones, with 93.60% accuracy, 94.48% precision, 92.60% recall, and 93.53% f-score. This is the first time that α-cuts were used in image processing and according to our results, we believe that they provide an important contribution to image processing for challenging datasets. Overall, it shows that it can become an integrated component of image-based IDS operations and other demanding real-time practices.
Task Complexity and the Skills Dilemma in the Programming and Control of Collaborative Robots for Manufacturing
Authors: George Peter , Cheng Chi-Tsun , Pang Toh Yen , Neville Katrina
Published: April 2023

While traditional industrial robots participate in repetitive manufacturing processes from behind caged safety enclosures, collaborative robots (cobots) offer a highly flexible and human-interactive solution to manufacturing automation. Rather than operating from within cages, safety features such as force and proximity sensors and programmed protection zones allow cobots to work safely, close to human workers. Cobots can be configured to either stop or slow their motion if they come in contact with a human or obstacle or enter a protection zone, which may be a high pedestrian traffic area. In this way, a task can be divided into sub-processes allocated to the cobot or the human based on suitability, capability or human preference. The flexible nature of the cobot makes it ideal for low-volume, ‘just-in-time’ manufacturing; however, this requires frequent reprogramming of the cobot to adapt to the dynamic processes. This paper reviews relevant cobot programming and control methods currently used in the manufacturing industry and alternative solutions proposed in the literature published from 2018 to 2023. The paper aims to (1) study the features and characteristics of existing cobot programming and control methods and those proposed in the literature, (2) compare the complexity of the task that the cobot is to perform with the skills needed to program it, (3) determine who is the ideal person to perform the programming role, and (4) assess whether the cobot programming and control methods are suited to that person’s skillset or if another solution is needed. The study is presented as a guide for potential adopters of cobots for manufacturing and a reference for further research.
Zero-Shot Relation Triple Extraction with Prompts for Low-Resource Languages
Authors: Halike Ayiguli , Wumaier Aishan , Yibulayin Tuergen
Published: April 2023

Although low-resource relation extraction is vital in knowledge construction and characterization, more research is needed on the generalization of unknown relation types. To fill the gap in the study of low-resource (Uyghur) relation extraction methods, we created a zero-shot with a quick relation extraction task setup. Each triplet extracted from an input phrase consists of the subject, relation type, and object. This paper suggests generating structured texts by urging language models to provide related instances. Our model consists of two modules: relation generator and relation and triplet extractor. We use the Uyghur relation prompt in the relation generator stage to generate new synthetic data. In the relation and triple extraction stage, we use the new data to extract the relation triplets in the sentence. We use multi-language model prompts and structured text techniques to offer a structured relation prompt template. This method is the first research that extends relation triplet extraction to a zero-shot setting for Uyghur datasets. Experimental results show that our method achieves a maximum weighted average F1 score of 47.39%.
Prediction of Mandibular Third Molar Impaction Using Linear and Angular Measurements in Young Adult Orthopantomograms
Authors: Mummolo Stefano , Gallusi Gianni , Strappa Enrico M. , Grilli Filippo , Mattei Antronella , Fiasca Fabiana , Bambini Fabrizio , Memè Lucia
Published: April 2023

This retrospective study aimed to evaluate a possible correlation between the characteristics of the mandibular ramus and lower third molar impaction by comparing a group of subjects with an impacted lower third molar and a second group with normal eruption for an early prediction of this pathology. This comparison was made using linear and angular measurements, which were taken on digital panoramic radiographs. Materials and methods: A total of 726 orthopantomographs (OPT) were examined, and 81 were considered suitable for the present study. The results were divided into two groups: a control group and an experimental group. The control group comprised 38 cases in which patients had at least one lower third molar that had erupted, and the experimental group comprised 43 cases in which patients had at least one lower third molar that was impacted or partially impacted. In total, 16 variables (11 linear, 4 angular, and 1 ratio) were determined and measured by an experienced observer. Results: The control group had a larger retromolar space, a larger impaction angle and a higher ratio of retromolar area to the third molar, compared to the experimental group. In contrast, the experimental group showed a deeper sigmoid notch depth than the control group did. In the control group, moderate positive correlations were found between both the length of the coronoid and the width of the third molar, and the retromolar space. Furthermore, in the experimental group, moderate positive correlations were found between both the angular condyle–coronoid process and the inclination of the lower posterior teeth, and the retromolar space. Conclusion: this study showed that the angle of a lower third molar, in relation to mandibular pain, can be an index for predicting tooth inclusion.
Multiple Factors Driving Carbonate System in Subtropical Coral Community Environments along Dapeng Peninsula, South China Sea
Authors: Yang Bo , Zhang Zhuo , Cui Zhouping , Xie Ziqiang , Chen Bogui , Zheng Huina , Liao Baolin , Zhou Jin , Xiao Baohua
Published: April 2023

Coral reef ecosystems have extremely high primary productivity and play an important role in the marine carbon cycle. However, due to the high carbon metabolism efficiency of coral communities, little is known about the carbon sink–source properties of coral reefs. In November 2022, in situ field investigations coupled with incubation experiments were conducted in typical subtropical coral reef waters, i.e., Yangmeikeng Sea Area (Area I) and Dalu Bay (Area Ⅱ), to explore the dynamics of the carbonate system and its controlling factors. The results revealed that the carbonate parameters had high variability, comprehensively forced by various physical and biochemical processes. Overall, Areas I and Ⅱ were net sinks of atmospheric CO2, with net uptake fluxes of 1.66 ± 0.40 and 0.99 ± 0.08 mmol C m−2 day−1, respectively. The aragonite saturation state (ΩA), 3.04–3.87, was within the range adequate for growth of tropical shallow-water scleractinian corals. Inorganic carbon budget results indicated that photosynthesis and microbial respiration were the main factors affecting the dynamics of carbonate systems in the whole study area. However, focusing on the reef areas, coral metabolism was also a key factor affecting the carbonate system in seawater (especially in Area I) and its contribution accounted for 28.9–153.3% of the microbial respiration. This study highlighted that metabolism of coral communities could significantly affect the seawater carbonate system, which is of great significance in the context of the current process of ocean acidification.
Evaluation of Convective Environments in the NARCliM Regional Climate Modeling System for Australia
Authors: Cheung Kevin K. W. , Ji Fei , Nishant Nidhi , Herold Nicholas , Cook Kellie
Published: April 2023

Severe thunderstorms lead to multiple hazards including torrential precipitation, flash flood, hail, lightning, and wind gusts. The meso- to micro-scale nature of thunderstorms impose great challenges from understanding individual storm dynamics, storm climatology as well as projecting their future activities. High-resolution regional climate models can resolve the convective environments better than global models. Australia, especially the east and southeast parts of the continent, is a global hot spot for severe thunderstorms. This study evaluates the simulated convective environments from the New South Wales (NSW) and Australian Regional Climate Modelling (NARCliM) project based on the parameters of CAPE, CIN, 0–6-km vertical wind shear and storm severity. The ensemble regional downscaling is compared against the fifth-generation European Centre for Medium-range Weather Forecast Reanalysis (ERA5). The results show that although there are apparent biases (generally positive for CAPE and negative for CIN, and slightly overestimated vertical wind shear) in the downscaled storm parameters, the climatology of measures of storm severity over land, including their spatial patterns and seasonality, agree well with ERA5. These results have strong implication on the application of the climate projection to assess future convective environments in the region.
GRASP Manual for Users
Authors: Jönsson Per , Gaigalas Gediminas , Fischer Charlotte Froese , Bieroń Jacek , Grant Ian P. , Brage Tomas , Ekman Jörgen , Godefroid Michel , Grumer Jon , Li Jiguang , Li Wenxian
Published: April 2023

grasp is a software package in Fortran 95, adapted to run in parallel under MPI, for research in atomic physics. The basic premise is that, given a wave function, any observed atomic property can be computed. Thus, the first step is always to determine a wave function. Different properties challenge the accuracy of the wave function in different ways. This software is distributed under the MIT Licence.
De Novo Synthesis of Poly(3-hydroxybutyrate-co-3-hydroxypropionate) from Oil by Engineered Cupriavidus necator
Authors: Li Mengdi , Li Wei , Zhang Tongtong , Guo Keyi , Feng Dexin , Liang Fengbing , Xu Chao , Xian Mo , Zou Huibin
Published: April 2023

Poly(3-hydroxybutyrate-co-3-hydroxypropionate) [P(3HB-co-3HP)] is a biodegradable and biocompatible polyester with improved and expanded material properties compared with poly(3-hydroxybutyrate) (PHB). This study engineered a robust malonyl-CoA pathway in Cupriavidus necator for the efficient supply of a 3HP monomer, and could achieve the production of [P(3HB-co-3HP)] from variable oil substrates. Flask level experiments followed by product purification and characterization found the optimal fermentation condition (soybean oil as carbon source, 0.5 g/L arabinose as induction level) in general consideration of the PHA content, PHA titer and 3HP molar fraction. A 5 L fed-batch fermentation (72 h) further increased the dry cell weight (DCW) to 6.08 g/L, the titer of [P(3HB-co-3HP)] to 3.11 g/L and the 3HP molar fraction to 32.25%. Further improving the 3HP molar fraction by increasing arabinose induction failed as the engineered malonyl-CoA pathway was not properly expressed under the high-level induction condition. With several promising advantages (broader range of economic oil substrates, no need for expensive supplementations such as alanine and VB12), this study indicated a candidate route for the industrial level production of [P(3HB-co-3HP)]. For future prospects, further studies are needed to further improve the strain and the fermentation process and expand the range of relative products.
Tripeptide IRW Improves AMPK/eNOS Signaling Pathway via Activating ACE2 in the Aorta of High-Fat-Diet-Fed C57BL/6 Mice
Authors: Ashkar Fatemeh , Bhullar Khushwant S. , Jiang Xu , Wu Jianping
Published: April 2023

This study aims to investigate the effect of tripeptide IRW on the local renin–angiotensin system (RAS), particularly angiotensin-converting enzyme 2 (ACE2), and their association with signaling pathways in the aorta of a high-fat-diet (HFD)-induced insulin-resistant mouse model. C57BL/6 mice were fed HFD (45% of the total calories) for six weeks, and then IRW was added to the diet (45 mg/kg body weight (BW)) for another eight weeks. ACE2 mRNA expression and protein level(s) were increased (p < 0.05), while angiotensin II receptor (AT1R) and angiotensin-converting enzyme (ACE) protein abundance was significantly reduced (p < 0.05) in the aorta of HFD mice treated by IRW. IRW supplementation also improved glucose transporter 4 (GLUT4) abundance (p < 0.05) alongside AMP-activated protein kinase (AMPK) (p < 0.05), Sirtuin 1 (SIRT1) (p < 0.05), and endothelial nitric oxide synthase (eNOS) (p < 0.05) expression. IRW downregulated the levels of endothelin 1 (ET-1) and p38 mitogen-activated protein kinases (p38 MAPK, p < 0.05). Furthermore, the levels of AMPK and eNOS in vascular smooth muscle cells (VSMCs) were significantly reduced in ACE2 knockdown cells treated with or without IRW (p < 0.01). In conclusion, this study provided new evidence of the regulatory role of IRW on the aortic ACE2 against metabolic syndrome (MetS) in an HFD-induced insulin-resistant model.
Adenosine Improves Mitochondrial Function and Biogenesis in Friedreich’s Ataxia Fibroblasts Following L-Buthionine Sulfoximine-Induced Oxidative Stress
Authors: Lew Sze Yuen , Mohd Hisam Nur Shahirah , Phang Michael Weng Lok , Syed Abdul Rahman Syarifah Nur , Poh Rozaida Yuen Ying , Lim Siew Huah , Kamaruzzaman Mohd Amir , Chau Sze Chun , Tsui Ka Chun , Lim Lee Wei , Wong Kah Hui
Published: April 2023

Adenosine is a nucleoside that is widely distributed in the central nervous system and acts as a central excitatory and inhibitory neurotransmitter in the brain. The protective role of adenosine in different pathological conditions and neurodegenerative diseases is mainly mediated by adenosine receptors. However, its potential role in mitigating the deleterious effects of oxidative stress in Friedreich’s ataxia (FRDA) remains poorly understood. We aimed to investigate the protective effects of adenosine against mitochondrial dysfunction and impaired mitochondrial biogenesis in L-buthionine sulfoximine (BSO)-induced oxidative stress in dermal fibroblasts derived from an FRDA patient. The FRDA fibroblasts were pre-treated with adenosine for 2 h, followed by 12.50 mM BSO to induce oxidative stress. Cells in medium without any treatments or pre-treated with 5 µM idebenone served as the negative and positive controls, respectively. Cell viability, mitochondrial membrane potential (MMP), aconitase activity, adenosine triphosphate (ATP) level, mitochondrial biogenesis, and associated gene expressions were assessed. We observed disruption of mitochondrial function and biogenesis and alteration in gene expression patterns in BSO-treated FRDA fibroblasts. Pre-treatment with adenosine ranging from 0–600 µM restored MMP, promoted ATP production and mitochondrial biogenesis, and modulated the expression of key metabolic genes, namely nuclear respiratory factor 1 (NRF1), transcription factor A, mitochondrial (TFAM), and NFE2-like bZIP transcription factor 2 (NFE2L2). Our study demonstrated that adenosine targeted mitochondrial defects in FRDA, contributing to improved mitochondrial function and biogenesis, leading to cellular iron homeostasis. Therefore, we suggest a possible therapeutic role for adenosine in FRDA.
Potential Geographic Range of the Endangered Reed Parrotbill Paradoxornis heudei under Climate Change
Authors: Chen Wan , Miao Keer , Guo Kun , Qian Weiya , Sun Wan , Wang Hao , Chang Qing , Hu Chaochao
Published: April 2023

The phenomenon of global climate change can impact the geographic range and biodiversity, thereby heightening the vulnerability of rare species to extinction. The reed parrotbill (Paradoxornis heudei David, 1872) is endemic to central and eastern China, it is mainly distributed in the middle and lower reaches of the Yangtze River Plain and the Northeast Plain. In this study, eight of ten algorithms of the species distribution model (SDM) were used to evaluate the impact of climate change on the potential distribution of P. heudei under current and future climate scenarios and to analyze the possible related climate factors. After checking the collected data, 97 occurrence records of P. heudei were used. The relative contribution rate shows that among the selected climatic variables, temperature annual range (bio7), annual precipitation (bio12), and isothermality (bio3) were the principal climatic factors to limit the habitat suitability of P. heudei. The suitable habitat for P. heudei is primarily concentrated in the central–eastern and northeast plains of China, particularly in the eastern coastal region, spanning a mere area of 57,841 km2. The habitat suitability of P. heudei under different representative concentration pathway (RCP) scenarios was predicted to be different under future climatic conditions, but all of them had a larger range than the current one. The species distribution range could expand by more than 100% on average compared with the current range under the four scenarios in 2050, while it could contract by approximately 30% on average relative to the 2050 range in 2070 under different climate change scenarios. In the future, northeastern China may serve as a potential suitable habitat for P. heudei. The changes in the spatial and temporal distributions of P. heudei’s range are of utmost importance in identifying high-priority conservation regions and devising effective management strategies for its preservation.
The eGFR Decline as a Risk Factor for Metabolic Syndrome in the Korean General Population: A Longitudinal Study of Individuals with Normal or Mildly Reduced Kidney Function
Authors: Han Seung Hyun , Lee Seung Ku , Shin Chol , Han Sang Youb
Published: April 2023

Metabolic syndrome (MS) is a risk factor for the development and progression of chronic kidney disease (CKD). However, it is unclear whether decreased renal function affects MS. Through a longitudinal study, we investigated the effect of estimated glomerular filtration rate (eGFR) changes on MS in participants with an eGFR above 60 mL/min/1.73 m2. A cross-sectional (n = 7107) and a 14-year longitudinal study (n = 3869) were conducted to evaluate the association between MS and eGFR changes from the Korean Genome and Epidemiology Study data. The participants were categorized by their eGFR levels (60–75, 75–90, and 90–105 versus ≥ 105 mL/min/1.73 m2). In a cross-sectional analysis, the MS prevalence was significantly increased with a decline in the eGFR in a fully adjusted model. The odds ratio of individuals with an eGFR of 60–75 mL/min/1.73 m2 was observed to be the highest (2.894; 95% confidence interval (CI), 1.984–4.223). In the longitudinal analysis, incident MS significantly increased with an eGFR decline in all the models, with the highest hazard ratio in the lowest eGFR group (1.803; 95% CI, 1.286–2.526). In joint interaction analysis, all covariates showed a significant joint effect with an eGFR decline on the incident MS. MS incidents are associated with eGFR changes in the general population without CKD.
The Value of Various Post-Processing Modalities of Diffusion Weighted Imaging in the Detection of Multiple Sclerosis
Authors: Alghamdi Ahmad Joman
Published: April 2023

Diffusion tensor imaging (DTI) showed its adequacy in evaluating the normal-appearing white matter (NAWM) and lesions in the brain that are difficult to evaluate with routine clinical magnetic resonance imaging (MRI) in multiple sclerosis (MS). Recently, MRI systems have been developed with regard to software and hardware, leading to different proposed diffusion analysis methods such as diffusion tensor imaging, q-space imaging, diffusional kurtosis imaging, neurite orientation dispersion and density imaging, and axonal diameter measurement. These methods have the ability to better detect in vivo microstructural changes in the brain than DTI. These different analysis modalities could provide supplementary inputs for MS disease characterization and help in monitoring the disease’s progression as well as treatment efficacy. This paper reviews some of the recent diffusion MRI methods used for the assessment of MS in vivo.
Walking Ability Associated with Executive Dysfunction in Patients with Stroke: A Cross-Sectional Study
Authors: Sakai Katsuya , Hosoi Yuichiro , Harada Yusuke
Published: April 2023

Previous studies have shown an association between executive dysfunction and walking ability. However, it remains unclear whether the degree of executive dysfunction is associated with differences in walking ability in patients with stroke. The aim of this study was to investigate whether there are differences in walking ability according to executive dysfunction in patients with stroke. A total of 51 patients with stroke were enrolled in this study. Executive function was measured using the Trail Making Test (TMT) Part B, and walking ability was assessed using the 10 m walk test and the Timed Up and Go Test (TUGT). Cluster analysis was performed using the TMT Part B and compared within each cluster. TMT Part B was categorized into three groups (cluster 1: n = 20, cluster 2: n = 24, and cluster 3: n = 7). Cluster 1 was significantly better than clusters 2 and 3, and cluster 2 was significantly better than cluster 3. The 10 m walk time and TUGT of cluster 1 were significantly better than those of cluster 3. However, the 10 m walk time and TUGT of clusters 1 and 2 did not differ significantly. In conclusion, these findings may indicate differences in walking ability according to executive dysfunction.
Emotional Dysfunction and Interoceptive Challenges in Adults with Autism Spectrum Disorders
Authors: Bonete Saray , Molinero Clara , Ruisanchez Daniela
Published: April 2023

People with autism spectrum disorder (ASD) frequently show impaired sensory processing in different senses, including the interoceptive system. Recent findings suggest that interoception is a fundamental component of emotional experience and that impaired interoception is associated with alexithymia. This study aims to explore the association and interrelation between interoceptive confusion, alexithymia, and the capacity for emotional regulation among a sample of 33 adults with ASD compared to a control group of 35 adults with neurotypical development and its mutual impact. The participants answered a series of questionnaires addressing these three variables. The results showed (1) significant differences between the groups in all dimensions, with dysfunctional emotional regulation, impaired interoception, and alexithymia in the ASD group, (2) significant correlations between interoceptive confusion, emotional clarity, and alexithymia in the ASD group but only positive correlations between interoceptive confusion and alexithymia in the CG, and (3) that emotional clarity, alexithymia, and autism explain 61% of the variance in interoceptive confusion. These results are in line with previous studies and suggest that training interoceptive ability may enhance emotional clarity and reduce alexithymia among those diagnosed with ASD, with significant implications in the planning of treatment.
Quantitative and Qualitative Analysis of Psychosocial Factors Affecting Women’s Entrepreneurship
Authors: Peris-Delcampo David , Núñez Antonio , Costa Catia Miriam , Moriconi Marcelo , Cantón Enrique , Garcia-Mas Alexandre
Published: April 2023

This work aims to clarify the psychosocial variables that lead women to undertake and those that prevent them from doing so. Two studies were conducted using a mixed methodology to compensate for the inherent weaknesses of using each approach. The first study was based on the collection of quantitative data using the GloPEW questionnaire with a sample of 296 people. The second study, of a qualitative nature, was carried out through focus groups with a sample of 26 people. The results show that self-efficacy and emotional intelligence are the main factors to develop to promote entrepreneurship among women. Although the data show statistical strength, it seems necessary to expand the sample and incorporate more profiles of female entrepreneurs, for example, with different levels of training, given the complexity and variety of intervening factors.
Changes in Oral Health Behavior According to Dental Calculus Removal Health Insurance in Korea
Authors: Kim Yu-Rin , Kang Hyun-Kyung
Published: April 2023

Background: Periodontal disease is a chronic disease that is increasing year by year. Korea also recognizes the seriousness of periodontal disease and has been applying preventive scaling in the National Health Insurance since 2013 to prevent it. Studies confirming the effectiveness of such insurance coverage are very scarce. Therefore, this study intended to confirm the effect of such policy by comparing and analyzing the oral health characteristics and oral health behavior of the South Koreans before and after the scaling insurance. Methods: For all the analyses, complex sampling analysis with the stratification variable, clustering variable, and weight was applied. For a total of 40,945 people, the demographic characteristics, oral health characteristics, dental clinic use, brushing, and use of oral care supplies were compared through chi-square tests. Results: Scaling insurance showed a positive effect (p < 0.05) on the unemployed and elderly people, who had lost their previous economic status; on smoking and willingness to quit smoking as well as on consultation on drinking problems; on dental-clinic use and oral examination in terms of utilization of dental clinics; and on brushing after lunch, before breakfast, and before sleeping. Conclusions: The study results showed that the scaling rate was universalized, and there was a positive effect on willingness to quit smoking and undergo oral examination. An active reimbursement policy for oral health education is needed, however, if a substantial change in oral health behavior is to be achieved.
The Association between a Minimum Amount of Physical Activity and Subsequent Muscle Strength and Balance in Older Adults: A Prospective Study
Authors: Li Yuan-Yu , Hsueh Ming-Chun , Park Jong-Hwan , Lai Ting-Fu , Hung Yi-Chuan , Liao Yung
Published: April 2023

This study aimed to investigate the association between 15 min of daily moderate-to-vigorous physical activity (MVPA) and subsequent muscle strength and balance in older adults. Data concerning community-dwelling older Taiwanese adults (mean age, 69.5 years) were collected during the baseline period (2018) and at a 12-month follow-up survey (2019). Time spent performing MVPA was objectively assessed using a triaxial accelerometer (ActiGraph wGT3x-BT) at baseline. Upper and lower limb muscle strength were measured using handgrip strength and a five-times sit-to-stand test, respectively. Balance was assessed using a one-leg standing test. The 12-month variations in muscle strength and balance were calculated through subtracting follow-up data from baseline data. A forced entry-adjusted logistic regression analysis was conducted. In total, 65.2% of participants engaged in at least 15 min of daily MVPA in the baseline survey. After adjusting for confounders, older adults who achieved 15 min of daily MVPA during the baseline period were more likely to maintain or improve balance performance (odds ratio, 8.12). Performing 15 min of daily MVPA was found to benefit older adults’ subsequent balance performance but not muscle strength.