bluuebunny/crossref_metadata_2025 huggingface dataset:
Fast Tearing Mode Driven by Demagnetized Electrons
Authors: Tsareva Olga O. , Leonenko Makar V. , Grigorenko Elena E. , Malova Helmi V. , Popov Victor Yu. , Zelenyi Lev M.
Published: April 2024

Recent MMS observations have discovered electron‐scale super‐thin current sheets (STCSs) with a partial electron demagnetization, which distinguishes them from the ion‐scale TCSs traditionally observed by the Cluster mission. Our investigation focuses on the dynamics of STCSs and reveals new aspects influencing their stability. We use the earlier proposed 1D collisionless self‐consistent equilibrium STCS model and show that the free parameters of this model, such as the relative part of demagnetized electrons, their flow velocity and the pressure anisotropy of magnetized electron population, can contribute to the development of tearing instability. With the growth of these parameters, the STCS becomes thinner, which leads to the accumulation excess of a free energy. Stabilizing energy decreases due to the increase of a relative part of demagnetized electrons. Thus, demagnetized electrons in STCSs can provide the development of fast and short‐wavelength electron tearing modes.
Direct Evidence of Drift‐Compressional Wave Generation in the Earth's Magnetosphere Detected by Arase
Authors: Yamamoto K. , Rubtsov A. V. , Kostarev D. V. , Mager P. N. , Klimushkin D. Yu. , Nosé M. , Matsuoka A. , Asamura K. , Miyoshi Y. , Yokota S. , Kasahara S. , Hori T. , Keika K. , Kasahara Y. , Kumamoto A. , Tsuchiya F. , Shoji M. , Nakamura S. , Shinohara I.
Published: April 2024

We present the first direct evidence of an in situ excitation of drift‐compressional waves driven by drift resonance with ring current protons in the magnetosphere. Compressional Pc4–5 waves with frequencies of 4–12 mHz were observed by the Arase satellite near the magnetic equator at L ∼ 6 in the evening sector on 19 November 2018. Estimated azimuthal wave numbers (m) ranged from −100 to −130. The observed frequency was consistent with that calculated using the drift‐compressional mode theory, whereas the plasma anisotropy was too small to excite the drift‐mirror mode. We discovered that the energy source of the wave was a drift resonance instability, which was generated by the negative radial gradient in a proton phase space density at 20–25 keV. This proton distribution is attributed to a temporal variation of the electric field, which formed the observed multiple‐nose structures of ring current protons.
Dynamics of Sputtered Neutral Sodium Atoms in the Near‐Mercury Space
Authors: Zhao J.‐T. , Zong Q.‐G. , Sun W.‐J. , Zhou X.‐Z. , Yue C. , Wang S. , Slavin J. A. , Raines J. M. , Wurz P. , Ip W.‐H.
Published: April 2024

The solar wind sputtering in the magnetospheric polar cusp is an important source of heavy atoms in Mercury’s exosphere and magnetosphere. However, the majority of ejected atoms are neutral, undergoing an extended period before photoionization occurs. In this study, we employ an ab initio simulation to investigate the behavior of sodium (Na) atoms prior to their photoionization. Our results reveal that overall only approximately 2.7% of the sputtered atoms contribute to magnetospheric ions, while the vast majority of these ions (∼82.9%) escape into interplanetary space. The remaining fraction (14.4%) eventually returns to the planetary surface. For Na atoms ionized inside the magnetosphere, a larger proportion of Na+ (53.5%) is supplied to the magnetotail compared to the polar cusp (39.4%), which is due to the tailward acceleration caused by solar radiation. Additionally, the remaining Na+ (7.1%) contributes to the dayside ring current region, as demonstrated by the observation of the MErcury Surface, Space ENvironment, GEochemistry, and Ranging (MESSENGER) spacecraft. Our research introduces a perspective on Na+ transport in the magnetosphere that complements and coexists with traditional mechanisms.
CALIPSO Observed Low Clouds Over the Subtropical Northeast Pacific: Summer Climatology and Interannual Variability
Authors: Liu Jing‐Wu , Yang Liu , Xie Shang‐Ping
Published: April 2024

This study examines the spatial distribution and temporal variations of low‐cloud top over the subtropical northeast Pacific (NEP), using observations from the Cloud‐Aerosol Lidar and Infrared Pathfinder Satellite Observations (CALIPSO). A comparison between CALIPSO observations and in‐situ soundings reveals a distinct bimodal distribution of low‐cloud tops, characterized by peaks near the inversion base and transition layer, corresponding to shallow cumulus clouds below the stratocumulus deck and boundary‐layer decoupling phenomena. Climatologically, while traversing along a great circle from Los Angeles to Honolulu, the frequency of low cloud occurrence experiences a gradual reduction from 80% to 30%. Meanwhile, cloud top height increases from 0.6 to 1.6 km, and surface roughness varies from 50 to 300 m. This study marks the first presentation of long‐term characteristics of low clouds over a vast, remote area, expanding beyond discrete field campaign observations. Accurately characterizing the marine atmospheric boundary layer (MABL) including its depth and degree of decoupling, enables further investigation into the interannual variations of low clouds, using 14 years of CALIPSO observations. Significant interannual variability in areal fraction and vertical bimodality of low clouds emerges downstream of the anomalously cold water; increased cloud cover with weakened bimodality is associated with a shallow weakly decoupled MABL. This research emphasizes the need to consider the downstream effects in studying low cloud‐sea surface temperature feedback within climate models.
Amygdalar neurotransmission alterations in the BTBR mice model of idiopathic autism
Authors: Bove Maria , Palmieri Maria Adelaide , Santoro Martina , Agosti Lisa Pia , Gaetani Silvana , Romano Adele , Dimonte Stefania , Costantino Giuseppe , Sikora Vladyslav , Tucci Paolo , Schiavone Stefania , Morgese Maria Grazia , Trabace Luigia
Published: April 2024

Autism Spectrum Disorders (ASD) are principally diagnosed by three core behavioural symptoms, such as stereotyped repertoire, communication impairments and social dysfunctions. This complex pathology has been linked to abnormalities of corticostriatal and limbic circuits. Despite experimental efforts in elucidating the molecular mechanisms behind these abnormalities, a clear etiopathogenic hypothesis is still lacking. To this aim, preclinical studies can be really helpful to longitudinally study behavioural alterations resembling human symptoms and to investigate the underlying neurobiological correlates. In this regard, the BTBR T+ Itpr3tf/J (BTBR) mice are an inbred mouse strain that exhibits a pattern of behaviours well resembling human ASD-like behavioural features. In this study, the BTBR mice model was used to investigate neurochemical and biomolecular alterations, regarding Nerve Growth Factor (NGF) and Brain-Derived Neurotrophic Factor (BDNF), together with GABAergic, glutamatergic, cholinergic, dopaminergic and noradrenergic neurotransmissions and their metabolites in four different brain areas, i.e. prefrontal cortex, hippocampus, amygdala and hypothalamus. In our results, BTBR strain reported decreased noradrenaline, acetylcholine and GABA levels in prefrontal cortex, while hippocampal measurements showed reduced NGF and BDNF expression levels, together with GABA levels. Concerning hypothalamus, no differences were retrieved. As regarding amygdala, we found reduced dopamine levels, accompanied by increased dopamine metabolites in BTBR mice, together with decreased acetylcholine, NGF and GABA levels and enhanced glutamate content. Taken together, our data showed that the BTBR ASD model, beyond its face validity, is a useful tool to untangle neurotransmission alterations that could be underpinned to the heterogeneous ASD-like behaviours, highlighting the crucial role played by amygdala.
Comparative evaluation of SNVs, indels, and structural variations detected with short- and long-read sequencing data
Authors: Kosugi Shunichi , Terao Chikashi
Published: April 2024

Short- and long-read sequencing technologies are routinely used to detect DNA variants, including SNVs, indels, and structural variations (SVs). However, the differences in the quality and quantity of variants detected between short- and long-read data are not fully understood. In this study, we comprehensively evaluated the variant calling performance of short- and long-read-based SNV, indel, and SV detection algorithms (6 for SNVs, 12 for indels, and 13 for SVs) using a novel evaluation framework incorporating manual visual inspection. The results showed that indel-insertion calls greater than 10 bp were poorly detected by short-read-based detection algorithms compared to long-read-based algorithms; however, the recall and precision of SNV and indel-deletion detection were similar between short- and long-read data. The recall of SV detection with short-read-based algorithms was significantly lower in repetitive regions, especially for small- to intermediate-sized SVs, than that detected with long-read-based algorithms. In contrast, the recall and precision of SV detection in nonrepetitive regions were similar between short- and long-read data. These findings suggest the need for refined strategies, such as incorporating multiple variant detection algorithms, to generate a more complete set of variants using short-read data.
Inflammation and cytomegalovirus viremia during pregnancy drive sex-differentiated differences in mortality and immune development in HIV-exposed infants
Authors: Evans Ceri , Mutasa Kuda , Rukobo Sandra , Govha Margaret , Mushayanembwa Patience , Chasekwa Bernard , Majo Florence D. , Tavengwa Naume V. , Broad Jonathan , Noble Christie , Gough Ethan K. , Kelly Paul , Bourke Claire D. , Humphrey Jean H. , Ntozini Robert , Prendergast Andrew J.
Published: April 2024

Children who are HIV-exposed but uninfected have increased infectious mortality compared to HIV-unexposed children, raising the possibility of immune abnormalities following exposure to maternal viraemia, immune dysfunction, and co-infections during pregnancy. In a secondary analysis of the SHINE trial in rural Zimbabwe we explored biological pathways underlying infant mortality, and maternal factors shaping immune development in HIV-exposed uninfected infants. Maternal inflammation and cytomegalovirus viraemia were independently associated with infant deaths: mortality doubled for each log10 rise in maternal C-reactive protein (adjusted hazard ratio (aHR) 2.09; 95% CI 1.33–3.27), and increased 1.6-fold for each log10 rise in maternal cytomegalovirus viral load (aHR 1.62; 95% CI 1.11–2.36). In girls, mortality was more strongly associated with maternal C-reactive protein than cytomegalovirus; in boys, mortality was more strongly associated with cytomegalovirus than C-reactive protein. At age one month, HIV-exposed uninfected infants had a distinct immune milieu, characterised by raised soluble CD14 and an altered CD8 + T-cell compartment. Alterations in immunophenotype and systemic inflammation were generally greater in boys than girls. Collectively, these findings show how the pregnancy immune environment in women with HIV underlies mortality and immune development in their offspring in a sex-differentiated manner, and highlights potential new intervention strategies to transform outcomes of HIV-exposed children. ClinicalTrials.gov/NCT01824940.
Noncovalent interaction with a spirobipyridine ligand enables efficient iridium-catalyzed C–H activation
Authors: Jin Yushu , Ramadoss Boobalan , Asako Sobi , Ilies Laurean
Published: April 2024

Exploitation of noncovalent interactions for recognition of an organic substrate has received much attention for the design of metal catalysts in organic synthesis. The CH–π interaction is especially of interest for molecular recognition because both the C–H bonds and the π electrons are fundamental properties of organic molecules. However, because of their weak nature, these interactions have been less utilized for the control of organic reactions. We show here that the CH–π interaction can be used to kinetically accelerate catalytic C–H activation of arenes by directly recognizing the π-electrons of the arene substrates with a spirobipyridine ligand. Computation and a ligand kinetic isotope effect study provide evidence for the CH–π interaction between the ligand backbone and the arene substrate. The rational exploitation of weak noncovalent interactions between the ligand and the substrate will open new avenues for ligand design in catalysis.
Reconstruction of macroglia and adult neurogenesis evolution through cross-species single-cell transcriptomic analyses
Authors: Morizet David , Foucher Isabelle , Alunni Alessandro , Bally-Cuif Laure
Published: April 2024

Macroglia fulfill essential functions in the adult vertebrate brain, producing and maintaining neurons and regulating neuronal communication. However, we still know little about their emergence and diversification. We used the zebrafish D. rerio as a distant vertebrate model with moderate glial diversity as anchor to reanalyze datasets covering over 600 million years of evolution. We identify core features of adult neurogenesis and innovations in the mammalian lineage with a potential link to the rarity of radial glia-like cells in adult humans. Our results also suggest that functions associated with astrocytes originated in a multifunctional cell type fulfilling both neural stem cell and astrocytic functions before these diverged. Finally, we identify conserved elements of macroglial cell identity and function and their time of emergence during evolution.
MmcA is an electron conduit that facilitates both intracellular and extracellular electron transport in Methanosarcina acetivorans
Authors: Gupta Dinesh , Chen Keying , Elliott Sean J. , Nayak Dipti D.
Published: April 2024

Methanogens are a diverse group of Archaea that obligately couple energy conservation to the production of methane. Some methanogens encode alternate pathways for energy conservation, like anaerobic respiration, but the biochemical details of this process are unknown. We show that a multiheme c-type cytochrome called MmcA from Methanosarcina acetivorans is important for intracellular electron transport during methanogenesis and can also reduce extracellular electron acceptors like soluble Fe3+ and anthraquinone-2,6-disulfonate. Consistent with these observations, MmcA displays reversible redox features ranging from −100 to −450 mV versus SHE. Additionally, mutants lacking mmcA have significantly slower Fe3+ reduction rates. The mmcA locus is prevalent in members of the Order Methanosarcinales and is a part of a distinct clade of multiheme cytochromes that are closely related to octaheme tetrathionate reductases. Taken together, MmcA might act as an electron conduit that can potentially support a variety of energy conservation strategies that extend beyond methanogenesis.
Ultrastrong exciton-plasmon couplings in WS2 multilayers synthesized with a random multi-singular metasurface at room temperature
Authors: Wu Tingting , Wang Chongwu , Hu Guangwei , Wang Zhixun , Zhao Jiaxin , Wang Zhe , Chaykun Ksenia , Liu Lin , Chen Mengxiao , Li Dong , Zhu Song , Xiong Qihua , Shen Zexiang , Gao Huajian , Garcia-Vidal Francisco J. , Wei Lei , Wang Qi Jie , Luo Yu
Published: April 2024

Van der Waals semiconductors exemplified by two-dimensional transition-metal dichalcogenides have promised next-generation atomically thin optoelectronics. Boosting their interaction with light is vital for practical applications, especially in the quantum regime where ultrastrong coupling is highly demanded but not yet realized. Here we report ultrastrong exciton-plasmon coupling at room temperature in tungsten disulfide (WS2) layers loaded with a random multi-singular plasmonic metasurface deposited on a flexible polymer substrate. Different from seeking perfect metals or high-quality resonators, we create a unique type of metasurface with a dense array of singularities that can support nanometre-sized plasmonic hotspots to which several WS2 excitons coherently interact. The associated normalized coupling strength is 0.12 for monolayer WS2 and can be up to 0.164 for quadrilayers, showcasing the ultrastrong exciton-plasmon coupling that is important for practical optoelectronic devices based on low-dimensional semiconductors.
A pair of atypical NLR-encoding genes confers Asian soybean rust resistance in soybean
Authors: Hao Qingnan , Yang Hongli , Chen Shuilian , Zhang Chanjuan , Chen Limiao , Cao Dong , Yuan Songli , Guo Wei , Yang Zhonglu , Huang Yi , Qu Yanhui , Qin Lucy , Sheng Xiaoyan , Wang Xueyan , Mitra Chandrani , Zhong Heng , Dawson John , Bumann Eric , Wang Wenling , Jiang Yaping , Tang Guozhu , Carlin Ryan , Chen Haifeng , Liu Qingli , Shan Zhihui , Zhou Xinan
Published: April 2024

Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is a devastating disease that is present in all major soybean-producing regions. The limited availability of resistant germplasm has resulted in a scarcity of commercial soybean cultivars that are resistant to the disease. To date, only the Chinese soybean landrace SX6907 has demonstrated an immune response to ASR. In this study, we present the isolation and characterization of Rpp6907-7 and Rpp6907-4, a gene pair that confer broad-spectrum resistance to ASR. Rpp6907-7 and Rpp6907-4 encode atypic nucleotide-binding leucine-rich repeat (NLR) proteins that are found to be required for NLR-mediated immunity. Genetic analysis shows that only Rpp6907-7 confers resistance, while Rpp6907-4 regulates Rpp6907-7 signaling activity by acting as a repressor in the absence of recognized effectors. Our work highlights the potential value of using Rpp6907 in developing resistant soybean cultivars.
Physiological DNA damage promotes functional endoreplication of mammary gland alveolar cells during lactation
Authors: Molinuevo Rut , Menendez Julien , Cadle Kora , Ariqat Nabeela , Choy Marie Klaire , Lagousis Cayla , Thomas Gwen , Strietzel Catherine , Bubolz J. W. , Hinck Lindsay
Published: April 2024

Lactation insufficiency affects many women worldwide. During lactation, a large portion of mammary gland alveolar cells become polyploid, but how these cells balance the hyperproliferation occurring during normal alveologenesis with terminal differentiation required for lactation is unknown. Here, we show that DNA damage accumulates due to replication stress during pregnancy, activating the DNA damage response. Modulation of DNA damage levels in vivo by intraductal injections of nucleosides or DNA damaging agents reveals that the degree of DNA damage accumulated during pregnancy governs endoreplication and milk production. We identify a mechanism involving early mitotic arrest through CDK1 inactivation, resulting in a heterogeneous alveolar population with regards to ploidy and nuclei number. The inactivation of CDK1 is mediated by the DNA damage response kinase WEE1 with homozygous loss of Wee1 resulting in decreased endoreplication, alveologenesis and milk production. Thus, we propose that the DNA damage response to replication stress couples proliferation and endoreplication during mammary gland alveologenesis. Our study sheds light on mechanisms governing lactogenesis and identifies non-hormonal means for increasing milk production.
Goal commitment is supported by vmPFC through selective attention
Authors: Holton Eleanor , Grohn Jan , Ward Harry , Manohar Sanjay G. , O’Reilly Jill X. , Kolling Nils
Published: April 2024

When striking a balance between commitment to a goal and flexibility in the face of better options, people often demonstrate strong goal perseveration. Here, using functional MRI (n = 30) and lesion patient (n = 26) studies, we argue that the ventromedial prefrontal cortex (vmPFC) drives goal commitment linked to changes in goal-directed selective attention. Participants performed an incremental goal pursuit task involving sequential decisions between persisting with a goal versus abandoning progress for better alternative options. Individuals with stronger goal perseveration showed higher goal-directed attention in an interleaved attention task. Increasing goal-directed attention also affected abandonment decisions: while pursuing a goal, people lost their sensitivity to valuable alternative goals while remaining more sensitive to changes in the current goal. In a healthy population, individual differences in both commitment biases and goal-oriented attention were predicted by baseline goal-related activity in the vmPFC. Among lesion patients, vmPFC damage reduced goal commitment, leading to a performance benefit.
Multi-year time series of daily solute and isotope measurements from three Swiss pre-Alpine catchments
Authors: Knapp Julia L. A. , Napitupulu Tracy , von Freyberg Jana , Rücker Andrea , Studer Bjørn , Zappa Massimiliano , Kirchner James W.
Published: April 2024

Time series analyses of solute concentrations in streamwater and precipitation are powerful tools for unraveling the interplay of hydrological and biogeochemical processes at the catchment scale. While such datasets are available for many sites around the world, they often lack the necessary temporal resolution or are limited in the number of solutes they encompass. Here we present a multi-year dataset encompassing daily records of major ions and a range of trace metals in both streamwater and precipitation in three catchments in the northern Swiss Pre-Alps. These time series capture the temporal variability observed in solute concentrations in response to storm events, snow melt, and dry summer conditions. This dataset additionally includes stable water isotope data as an extension of a publicly available isotope dataset collected concurrently at the same locations, and together these data can provide insights into a range of ecohydrological processes and enable a suite of analyses into hydrologic and biogeochemical catchment functioning.
High spatial resolution elevation change dataset derived from ICESat-2 crossover points on the Tibetan Plateau
Authors: Chen Tengfei , Wang Jian , Che Tao , Hao Xiaohua , Li Hongyi
Published: April 2024

Understanding elevation changes on the Tibetan Plateau is crucial to comprehend the changes in topography, landscape, climate, environmental conditions, and water resources. However, some of the current products that track elevation changes only cover specific surface types or limited areas, and others have low spatial resolution. We propose an algorithm to extract ICESat-2 crossover points dataset for the Tibetan Plateau, and form a dataset. The crossover points dataset has a density of 2.015 groups/km², and each group of crossover points indicates the amount of change in elevation before and after a period of time over an area of approximately 17 meters in diameter. Comparing ICESat-2 crossover points data with existing studies on glaciers and lakes, we demonstrated the reliability of the derived elevation changes. The ICESat-2 crossover points provide a refined data source for understanding high-spatial-resolution elevation changes on the Tibetan Plateau. This dataset can provide validation data for various studies that require high-precision or high-resolution elevation change data on the Tibetan Plateau.
Distinct alterations in white matter properties and organization related to maternal treatment initiation in neonates exposed to HIV but uninfected
Authors: Magondo Ndivhuwo , Meintjes Ernesta M. , Warton Fleur L. , Little Francesca , van der Kouwe Andre J. W. , Laughton Barbara , Jankiewicz Marcin , Holmes Martha J.
Published: April 2024

HIV exposed-uninfected (HEU) infants and children are at risk of developmental delays as compared to HIV uninfected unexposed (HUU) populations. The effects of exposure to in utero HIV and ART regimens on the HEU the developing brain are not well understood. In a cohort of 2-week-old newborns, we used diffusion tensor imaging (DTI) tractography and graph theory to examine the influence of HIV and ART exposure in utero on neonate white matter integrity and organisation. The cohort included HEU infants born to mothers who started ART before conception (HEUpre) and after conception (HEUpost), as well as HUU infants from the same community. We investigated HIV exposure and ART duration group differences in DTI metrics (fractional anisotropy (FA) and mean diffusivity (MD)) and graph measures across white matter. We found increased MD in white matter connections involving the thalamus and limbic system in the HEUpre group compared to HUU. We further identified reduced nodal efficiency in the basal ganglia. Within the HEUpost group, we observed reduced FA in cortical-subcortical and cerebellar connections as well as decreased transitivity in the hindbrain area compared to HUU. Overall, our analysis demonstrated distinct alterations in white matter integrity related to the timing of maternal ART initiation that influence regional brain network properties.
Identification of prevalent leachate percolation of municipal solid waste landfill: a case study in India
Authors: Alam Pervez , Khan Afzal Husain , Islam Raisul , Sabi Ehab , Khan Nadeem A. , Zargar Tasneem Imtiyaz
Published: April 2024

Landfill leachate forms when waste-inherent water and percolated rainfall transfer are highly toxic, corrosive, acidic, and full of environmental pollutants. The release of leachate from municipal solid waste (MSW) landfill sites poses a severe hazard to human health and aquatic life. This study examined the impact of leachate from Delhi’s Ghazipur landfill on the nearby groundwater quality. Analysis of leachate samples was done to determine various parameters such as total dissolved solids (TDS), hardness, alkalinity, electrical conductivity, pH, BOD5, COD, nitrate, sulphate, chloride and iron, and presence of coliform bacteria. Significant dissolved elements (22,690–34,525 mg/L) were observed in the samples, indicated by the high conductivity value (1156–1405 mho/cm). However, a stable pH range (6.90–7.80) of leachate samples was observed due to high alkalinity concentrations between 2123 and 3256 mg/L. The inverse distance weighing (IDW) interpolation tool from QGIS 3.22.7 developed spatial interpolated models for each parameter across the Ghazipur area. The IDW interpolated graphs of various parameters over the whole study area confirmed these contaminations. In addition, leachate and groundwater samples were physio-chemically analyzed, and temporal fluctuation in landfill waste has also been studied. The temporal fluctuation results showed that when heat is produced, transmitted, and lost throughout the waste system, the maximum temperature position fluctuates over time. The findings of this study highlight the critical importance of landfill management in reducing groundwater contamination from MSW leachate.
A comparative study on eDNA-based detection of Siamese bat catfish (Oreoglanis siamensis) in wet and dry conditions
Authors: Osathanunkul Maslin , Suwannapoom Chatmongkon
Published: April 2024

The use of environmental DNA (eDNA) analysis has demonstrated notable efficacy in detecting the existence of freshwater species, including those that are endangered or uncommon. This application holds significant potential for enhancing environmental monitoring and management efforts. However, the efficacy of eDNA-based detection relies on several factors. In this study, we assessed the impact of rainfall on the detection of eDNA for the Siamese bat catfish (Oreoglanis siamensis). Quantitative polymerase chain reaction (qPCR) analysis indicated that samples from days with average rainfall exceeding 35 mm (classified as heavy and very heavy rain) yielded negative results. While eDNA detection remains feasible on light or moderate rainy days, a noteworthy reduction in eDNA concentration and qPCR-positive likelihood was observed. Analysis across 12 sampling sites established a statistically significant negative relationship (p < 0.001) between eDNA detection and rainfall. Specifically, for each 1 mm increase in rainfall, there was an observed drop in eDNA concentration of 0.19 copies/mL (±0.14). The findings of this study provide definitive evidence that precipitation has a significant impact on the detection of eDNA in Siamese bat catfish. However, in the case of adverse weather conditions occurring on the day of sampling, our research indicates that it is acceptable to continue with the task, as long as the rainfall is not heavy or very heavy. To enhance the effectiveness of an eDNA survey, it is crucial to consider many factors related to climatic conditions. The aforementioned factor holds significant importance not only for the specific species under scrutiny but also for the broader dynamics of the climate.
The efficacy of green silica nanoparticles synthesized from rice straw in the management of Callosobruchus maculatus (Col., Bruchidae)
Authors: Ibrahim Samar Sayed , Elbehery Huda Hassan , Samy Ahmed
Published: April 2024

Rice straw, a byproduct of harvesting rice, must be disposed of by farmers in a variety of ways, including burning, which is hazardous for the environment. To address this issue, the straw needs to be utilized and turned into valuable products. One such product is nano-silica (SNPs), which will be synthesized and investigated in our study as a safe alternative to chemical insecticides. Rice straw-derived SNPs were synthesized using the Sol–Gel method. The contact toxicity of SNPs on Callosobruchus maculatus, a major pest of cowpea seeds, has been assessed. The size of synthesized SNPs was determined by transmission electron microscopy to be ~ 4 nm. The SNPs estimated LC50 on C. maculatus adults was 88.170 ppm after 48h exposure. By raising the tested concentration, SNPs treatment increased the mortality%, which reached 100% at 200 ppm exposures. Additionally, SNPs at LC50 treatment decreased adult longevity and the average number of emerged adults. The findings also verified that SNPs had no phytotoxic effects on the cowpea seeds germination. Rather, their application improved seed germination efficacy. This study proposed that rice straw can be utilized to manufacture highly efficient SNPs which can be efficiently employed to preserve stored grains from C. maculatus infestation.
Clinical decision making when cytology indicates a Warthin tumor
Authors: Sirviö Minna , Aro Katri , Naukkarinen Mira , Mäkitie Antti , Tarkkanen Jussi , Kelppe Jetta , Atula Timo
Published: April 2024

Warthin tumor (WT) is a benign tumor usually affecting the parotid gland. The main diagnostic tool remains ultrasound combined with fine-needle aspiration cytology (FNAC). This study aims to examine how reliably FNAC indicates WT for clinical decision making regarding surgical versus conservative management. We included all patients who underwent FNAC from a parotid gland lesion between 2016 and 2018 at our institution, and whose FNAC revealed WT suspicion. The FNACs were divided into three groups based on the cytology report: certain, likely, and possible WT. The patients were divided into two groups based on having had either surgery or follow-up. We sent a questionnaire to patients who had not undergone surgery in order to obtain follow-up for a minimum of four years. Altogether, 135 FNAC samples, from 133 tumors and 125 patients, showed signs of WT. Of the 125 patients, 44 (35%) underwent surgery, and 81 (65%) were managed conservatively. Preoperative misdiagnosis in FNAC occurred in three (7%) surgically treated tumors. Their FNACs were reported as possible WTs, but histopathology revealed another benign lesion. In the conservatively treated group, two patients underwent surgery later during the follow-up. Cytological statements of WT were seldom false, and none were malignant. The majority of the patients were only followed-up and rarely required further treatment. A certain or likely diagnosis of WT in the FNAC report by an experienced head and neck pathologist is highly reliable in selecting patients for conservative surveillance.
Inaccurate diagnosis of diabetes type in youth: prevalence, characteristics, and implications
Authors: Tosur Mustafa , Huang Xiaofan , Inglis Audrey S. , Aguirre Rebecca Schneider , Redondo Maria J.
Published: April 2024

Classifying diabetes at diagnosis is crucial for disease management but increasingly difficult due to overlaps in characteristics between the commonly encountered diabetes types. We evaluated the prevalence and characteristics of youth with diabetes type that was unknown at diagnosis or was revised over time. We studied 2073 youth with new-onset diabetes (median age [IQR] = 11.4 [6.2] years; 50% male; 75% White, 21% Black, 4% other race; overall, 37% Hispanic) and compared youth with unknown versus known diabetes type, per pediatric endocrinologist diagnosis. In a longitudinal subcohort of patients with data for ≥ 3 years post-diabetes diagnosis (n = 1019), we compared youth with steady versus reclassified diabetes type. In the entire cohort, after adjustment for confounders, diabetes type was unknown in 62 youth (3%), associated with older age, negative IA–2 autoantibody, lower C-peptide, and no diabetic ketoacidosis (all, p < 0.05). In the longitudinal subcohort, diabetes type was reclassified in 35 youth (3.4%); this was not statistically associated with any single characteristic. In sum, among racially/ethnically diverse youth with diabetes, 6.4% had inaccurate diabetes classification at diagnosis. Further research is warranted to improve accurate diagnosis of pediatric diabetes type.
High-performance cementitious composites containing nanostructured carbon additives made from charred coal fines
Authors: Gao Yuan , Pham Viet Hung , Weidman Jennifer , Kim Ki-Joong , Spaulding Richard E. , Wang Congjun , Matranga Christopher S.
Published: April 2024

Carbon-based nanomaterials, such as carbon nanoplatelets, graphene oxide, and carbon quantum dots, have many possible end-use applications due to their ability to impart unique mechanical, electrical, thermal, and optical properties to cement composites. Despite this potential, these materials are rarely used in the construction industry due to high material costs and limited data on performance and durability. In this study, domestic coal is used to fabricate low-cost carbon nanomaterials that can be used economically in cement formulations. A range of chemical and physical processing approaches are employed to control the size, morphology, and chemical functionalization of the carbon nanomaterial, which improves its miscibility with cement formulations and its impact on mechanical properties and durability. At loadings of 0.01 to 0.07 wt.% of coal-derived carbon nanomaterial, the compressive and flexural strength of cement samples are enhanced by 24% and 23%, respectively, in comparison to neat cement. At loadings of 0.02 to 0.06 wt.%, the compressive and flexural strength of concrete composites increases by 28% and 21%, respectively, in comparison to neat samples. Additionally, the carbon nanomaterial additives studied in this work reduce cement porosity by 36%, permeability by 86%, and chloride penetration depth by 60%. These results illustrate that low-loadings of coal-derived carbon nanomaterial additives can improve the mechanical properties, durability, and corrosion resistance of cement composites.
Interventions for anxiety and depression in patients with atopic dermatitis: a systematic review and meta-analysis
Authors: Hartono Stella P. , Chatrath Sheena , Aktas Ozge N , Kubala Stephanie A , Capozza Korey , Myles Ian A. , Silverberg Jonathan I. , Schwartz Alan
Published: April 2024

Atopic dermatitis (AD) is a chronic inflammatory skin disease that is associated with anxiety and depression. Few studies have addressed interventions for symptoms of anxiety and depression in this population. To determine the efficacy of interventions for anxiety and depression in patients with AD. PubMed, MEDLINE, EMBASE, and PsycINFO were searched from inception to November 2023. English-language studies published in peer-reviewed journals evaluating the effect of interventions on anxiety and/or depression using validated assessment tools on patients with AD were included. Titles, abstracts, and articles were screened by at least two independent reviewers. Of 1410 references that resulted in the initial search, 17 studies were included. Fourteen of these studies are randomized controlled trials, while the other 3 studies are prospective controlled trials with pre and post-test designs. Data were extracted using a standardized extraction form, and the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines were followed. To accommodate trials with multiple interventions (each compared to a control group), we conducted a mixed-effects meta-analysis with the trial as a random effect. Prespecified outcomes were changes in symptoms of anxiety and depression in patients with AD as evaluated using standardized assessment tools. Of the 17 studies included in this systematic review, 7 pharmacological intervention studies with 4723 participants examining 5 different medications were included in a meta-analysis. Of these studies, only 1 study evaluated medications prescribed to treat anxiety and/or depression; the rest evaluated medications prescribed to treat AD. Meta-analysis of all the pharmacological interventions resulted in significant improvement in anxiety, depression, and combined anxiety-depression scale scores (standardized mean difference [95% CI]: − 0.29 [− 0.49 to − 0.09], − 0.27 [− 0.45 to − 0.08], − 0.27 [− 0.45 to − 0.08]) respectively. The 10 non-pharmacological studies with 2058 participants showed general improvement in anxiety but not depression. A meta-analysis of the non-pharmacological interventions was not conducted due to variable approaches and limited data. Pharmacological interventions designed to improve AD were found to improve anxiety and depression in patients with moderate-severe disease. More comprehensive studies on non-pharmacological and pharmacological interventions that primarily target anxiety and depression are needed.
Unravelling the effect of droplet size on lipid oxidation in O/W emulsions by using microfluidics
Authors: ten Klooster Sten , Boerkamp Vincent J. P. , Hennebelle Marie , van Duynhoven John P. M. , Schroën Karin , Berton-Carabin Claire C.
Published: April 2024

Lipid oxidation in emulsions is hypothesised to increase with decreasing droplet size, as this increases the specific oil–water interfacial area, where lipid oxidation is expected to be initiated. In literature, however, contradictory results have been reported, which can be caused by confounding factors such as the oil droplet polydispersity and the distribution of components between the available phases. In this work, monodisperse surfactant-stabilised emulsions with highly controlled droplet sizes of 4.7, 9.1, and 26 µm were produced by microfluidic emulsification. We show that lipid oxidation increases with decreasing droplet size, which we ascribe to the increased contact area between lipids and continuous phase prooxidants. Besides, a significant amount of oxygen was consumed by oxidation of the surfactant itself (Tween 20), an effect that also increased with decreasing droplet size. These insights substantiate the importance of controlling droplet size for improving the oxidative stability of emulsions.
Ecosystem services potential is declining across European capital metropolitan areas
Authors: Hysa Artan , Löwe Roland , Geist Juergen
Published: April 2024

Ecosystem services (ES) are essential to sustainable development at multiple spatial scales. Monitoring ES potential (ESP) at the metropolitan level is imperative to sustainable cities. We developed a procedure for long-term monitoring of metropolitan ESP dynamics, utilizing open-source land use land cover (LULC) data and the expert matrix method. We compared the ESP results of 38 European Capital Metropolitan Areas (ECMA) regarding biodiversity integrity, drinking water provision, flood protection, air quality, water purification, and recreation & tourism. Our results show significant declines in ESP across ECMA due to LULC alteration between 2006, 2012, and 2018. We found that ECMA in post-socialist European countries like Poland (Warszawa) have experienced high rates of land use transformation with a remarkable impact on ESP. Surprisingly, we found that Fennoscandinan ECMA, like Helsinki, Stockholm, and Oslo which lead the cumulative ESP ranking, faced the ESP reduction of the highest impact in recent years. The correlation analysis of ESP dynamics to urban expansion and population growth rates suggests that inattentive urbanization processes impact ESP more than population growth. We unveil the implications of our results to the EU and global level agendas like the European Nature Conservation Law and the Sustainable Development Goals.
Effect of resistance circuit training on comprehensive health indicators in older adults: a systematic review and meta-analysis
Authors: Hu Chenxi , Xia Yunpeng , Zeng Dongye , Ye Mingyi , Mei Tao
Published: April 2024

The aging process leads to the degeneration of body structure and function. The objective of this study is to conduct a systematic review and meta-analysis of the effects of resistance circuit training (RCT) on comprehensive health indicators of older adults. PubMed, Embase, and Web of Science were searched until August 2023. Primary outcomes were body composition, muscle strength, cardiorespiratory endurance, blood pressure, and functional autonomy. Muscle function and exercise intensity subgroups were analyzed. RCT reduces body fat (MD = − 5.39 kg, 95% CI − 10.48 to − 0.29), BMI (MD = − 1.22, 95% CI − 2.17 to − 0.26), and body weight (MD = − 1.28 kg, 95% CI − 1.78 to − 0.78), and increases lean body mass (MD = 1.42 kg, 95% CI 0.83–2.01) in older adults. It improves upper limb strength (SMD = 2.09, 95% CI 1.7–2.48), lower limb strength (SMD = 2.03, 95% CI 1.56–2.51), cardiorespiratory endurance (MD = 94 m, 95% CI 25.69–162.67), and functional autonomy (MD = − 1.35, 95% CI − 1.73 to − 0.96). High-intensity RCT benefits BMI and body weight, while low-intensity exercise reduces blood pressure. RCT improves muscle function in push, pull, hip, and knee movements in older adults. RCT improves body composition, muscle strength, cardiorespiratory endurance, blood pressure, and functional autonomy in older adults. High-intensity training is superior for body composition, while moderate to low intensity training is more effective for lowering blood pressure.
Global longitudinal strain is an informative index of left ventricular performance in neonates receiving intensive care
Authors: Petoello Enrico , Flore Alice Iride , Nogara Silvia , Bonafiglia Elena , Lenzi Maria Beatrice , Arnone Olivia C. , Benfari Giovanni , Ciarcià Martina , Corsini Iuri , De Waal Koert , Gottin Leonardo , Ficial Benjamim
Published: April 2024

Echocardiographic assessment of left ventricular function is crucial in NICU. The study aimed to compare the accuracy and agreement of global longitudinal strain (GLS) with conventional measurements. Real-life echocardiograms of neonates receiving intensive care were retrospectively reviewed. Shortening fraction (SF), ejection fraction (EF) and S’ measurements were retrieved from health records. GLS was calculated offline from stored images. The association with stroke volume indexed for body weight (iSV) was evaluated by regression analysis. The diagnostic ability to identify uncompensated shock was assessed by ROC curve analysis. Cohen's κ was run to assess agreement. 334 echocardiograms of 155 neonates were evaluated. Mean ± SD gestational age and birth weight were 34.5 ± 4.1 weeks and 2264 ± 914 g, respectively. SF, EF, S’ and GLS were associated with iSV with R2 of 0.133, 0.332, 0.252 and 0.633, (all p < .001). Including all variables in a regression model, iSV prediction showed an adjusted R2 of 0.667, (p < .001). GLS explained 73% of the model variance. GLS showed a better ability to diagnose uncompensated shock (AUC 0.956) compared to EF, S’ and SF (AUC 0.757, 0.737 and 0.606, respectively). GLS showed a moderate agreement with EF (κ = .500, p < .001) and a limited agreement with S’ and SF (κ = .260, p < .001, κ = .242, p < .001). GLS was a more informative index of left ventricular performance, providing the rationale for a more extensive use of GLS at the cotside.
Changes in the land-use landscape pattern and ecological network of Xuzhou planning area
Authors: Zhou Xi , Chu Zuoyong , Ji Xiang
Published: April 2024

Ongoing rapid urbanization has triggered significant changes in land use, rendering landscape patterns adversely impacted and certain habitat patches degraded. Ecological networks have consequently contracted overall. As such, an investigation into how land-use landscape patterns and ecological networks change over time and space is of major significance for ecological restoration and regional sustainability. Taking Xuzhou Planning Area as a case study, we examined spatiotemporal changes and features of the landscape pattern by employing the land-use change degree, the land-use transition matrix, and quantified landscape pattern indices. An ecological network analysis, which studies the changes in network connectivity and robustness, as well as their causes and contributors, was undertaken to probe into the features and trends of spatiotemporal changes in the land-use landscape pattern and ecological network amid expeditious urbanization. Analysis results unveiled the following: (1) From 1985 to 2020, there was a decline in the area of farmland, forest, and grassland, accompanied by an increase in land for construction, water bodies, and unused land. The southwestern research area witnessed farmland substantially give way to land for construction for this period, and the most dramatic change in land use occurred between 2000 and 2010. (2) The area of dominant patches in the research area shrank, along with more fragmented, complex landscapes. The land for construction was emerging as the dominant landscape by area, whereas patches of farmland, forest, grassland, and water bodies became less connected. (3) The ecological network was densely linked in the northeast, with sparser connections in the southwest. Spatial shrinkage was observed in the research area’s southwestern and central ecological corridors. Overall, the number of ecological sources and corridors rose and subsequently dropped before a rebound. (4) The ecological network grew more connected and robust from 1985 through 1990, as portions of farmland were converted into water bodies, which led to an increase in ecological sources. Given a reduction in ecological sources and corridors in the southwestern and central regions between 1990 and 2010, network connectivity and robustness declined, which was reversed from 2010 onward with the addition of two ecological sources—Pan’an Lake and Dugong Lake. With an optimal ecological network in 1990, however, it deteriorated significantly by 2010. The research area saw the minimum value of its network connectivity indices of network stability index (α), evenness index (β), and connectivity index (γ), in 2010, when its ecological network was highly fragmented and vulnerable, attributing to a strong contrast between the maximal connected subgraph’s relative size and connectivity robustness. The research findings can lay scientific groundwork for addressing ecological issues, restoring landscape patterns, and developing ecological networks amid urbanization.