bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of the Establishment of the Mount Wuyi National Park on the Livelihood of Farmers
Authors: Yang Zhen , Ren Jinjie , Zhang Dahong
Published: August 2023

The construction of national parks has a profound impact on the production and life of surrounding farmers. Based on the survey data of 354 farmers around the Mount Wuyi National Park, this paper uses the method of constructing a difference-in-difference model to analyze the impact of the Mount Wuyi National Park construction on the livelihood choices of surrounding farmers and the main income of families. In addition, this article analyzes the heterogeneity of surrounding farmers based on differences in tea planting area and farmers’ income levels. The results show the following: First, compared with pre-establishment, farmers around the Mount Wuyi National Park still prefer tea-based agricultural employment or part-time employment. Second, after the establishment of the Mount Wuyi National Park, the main income of surrounding farmers’ families is still agricultural income. Therefore, the authors of this article believe that it is necessary to further strengthen the protection of the livelihoods of surrounding farmers and moderately create more livelihood choices for surrounding farmers in order to achieve both ecological and economic benefits.
Real-Time Localization and Colorful Three-Dimensional Mapping of Orchards Based on Multi-Sensor Fusion Using Extended Kalman Filter
Authors: Zhang Yibo , Sun Hao , Zhang Fanhang , Zhang Baohua , Tao Shutian , Li Haitao , Qi Kaijie , Zhang Shaoling , Ninomiya Seishi , Mu Yue
Published: August 2023

To realize autonomous navigation and intelligent management in orchards, vehicles require real-time positioning and globally consistent mapping of surroundings with sufficient information. However, the unstructured and unstable characteristics of orchards present challenges for accurate and stable localization and mapping. This study proposes a framework fusing LiDAR, visual, and inertial data by using the extended Kalman filter (EKF) to achieve real-time localization and colorful LiDAR point-cloud mapping in orchards. First, the multi-sensor data were integrated into a loosely-coupled framework based on the EKF to improve the pose estimation, with the pose estimation from LiDAR and gyroscope acting as the predictions, while that from visual-inertial odometry acting as the observations. Then, the Loam_Livox algorithm was enhanced by incorporating color from the image into the LiDAR point cloud, enabling the real-time construction of a three-dimensional colorful map of the orchard. The method demonstrates a high accuracy for localization in different motion trajectories (average RMSE: 0.3436) and different scenarios (average RMSE: 0.1230) and clear and efficient construction of three-dimensional colorful mapping, taking only 75.01 ms in localization and mapping for a frame of LiDAR point cloud. This indicates the proposed method has a great potential for the autonomous navigation of agricultural vehicles.
Effects of Diets Containing Different Levels of Copper, Manganese, and Iodine on Rumen Fermentation, Blood Parameters, and Growth Performance of Yaks
Authors: Lu Huizhen , Wu Weibin , Zhao Xinsheng , Abbas Musaddiq Wada , Liu Shujie , Hao Lizhuang , Xue Yanfeng
Published: August 2023

Copper, manganese, and iodine are part of a yak’s required trace elements. However, knowledge about their dietary requirements is scarce. Therefore, an experiment was conducted to evaluate rumen fermentation, blood parameters, and growth performance and screen out the optimum levels of trace elements in yaks’ diet. Here, 18 three-year-old castrated yaks were randomly divided into four groups, which fed with diets containing basal (CON: 4.40, 33.82, and 0 mg/kg) and low-level (LL: 10.00, 40.00, and 0.30 mg/kg), middle-level (ML: 15.00, 50.00, and 0.50 mg/kg), and high-level (HL: 20.00, 60.00, and 0.70 mg/kg) copper, manganese, and iodine for 30 days. With the increase in trace elements, yaks’ daily weight gain (DWG), rumen pH, ammonia nitrogen, microbial protein (MCP), and volatile fatty acids levels and serum triglycerides and urea nitrogen levels showed firstly increasing and then decreasing trends and reached the highest values in ML, and serum ceruloplasmin and total superoxide dismutase (T-SOD) activities showed continuously increasing trends. Yaks’ DWG, rumen MCP, butyrate, and valerate levels and serum triglycerides, urea nitrogen, ceruloplasmin, and T-SOD levels in ML were significantly higher than CON. Therefore, the recommended levels of copper, manganese, and iodine in growing yaks’ diet are 15.00, 50.00, and 0.50 mg/kg (ML), respectively.
Evaluation of Enterococcal Probiotic Usage and Review of Potential Health Benefits, Safety, and Risk of Antibiotic-Resistant Strain Emergence
Authors: Im Eric Jeeho , Lee Harry Hyun-Yup , Kim Minzae , Kim Myo-Kyoung
Published: August 2023

Enterococci are often used in probiotics but can also cause nosocomial infections. As such, enterococcal consumption may have beneficial health effects, but a thorough evaluation of virulence absence and risk of antibiotic resistance spread is needed at the strain level. This article reviewed ten online health product shopping websites in the US. On these websites, 23 probiotic products using enterococci were found across 12 companies. In addition, this article reviewed studies that demonstrated the probiotic potential of enterococcal consumption (e.g., gastrointestinal and respiratory disease, hyperlipidemia alleviation, as well as infection prevention). To investigate the safety aspects of enterococci, the present work examined studies evaluating virulence factors and antibiotic resistance. Furthermore, this article assessed research that explored these virulent factors, specifically in probiotics containing enterococci, as well as the potential transfer mechanism of their antibiotic resistance. Based on reviewed data, enterococcal probiotic consumption has been proven beneficial for conditions or symptoms of multiple diseases without any apparent adverse effects. However, due to the plasmid- or transposon-mediated gene transfer ability of enterococci, surveillance monitoring and further studies regarding enterococcal consumption are warranted. Future studies that identify enterococcal strains safe to use in probiotics without virulence factors and antibiotic resistance are imperative for evidence-based decisions by health organizations and government agencies.
Roles of Oxidative Stress in Synaptic Dysfunction and Neuronal Cell Death in Alzheimer’s Disease
Authors: Plascencia-Villa Germán , Perry George
Published: August 2023

Alzheimer’s disease (AD) is a brain disorder that progressively undermines memory and thinking skills by affecting the hippocampus and entorhinal cortex. The main histopathological hallmarks of AD are the presence of abnormal protein aggregates (Aβ and tau), synaptic dysfunction, aberrant proteostasis, cytoskeletal abnormalities, altered energy homeostasis, DNA and RNA defects, inflammation, and neuronal cell death. However, oxidative stress or oxidative damage is also evident and commonly overlooked or considered a consequence of the advancement of dementia symptoms. The control or onset of oxidative stress is linked to the activity of the amyloid-β peptide, which may serve as both antioxidant and pro-oxidant molecules. Furthermore, oxidative stress is correlated with oxidative damage to proteins, nucleic acids, and lipids in vulnerable cell populations, which ultimately lead to neuronal death through different molecular mechanisms. By recognizing oxidative stress as an integral feature of AD, alternative therapeutic or preventive interventions are developed and tested as potential or complementary therapies for this devastating neurodegenerative disease.
Predicting the Surface Soil Texture of Cultivated Land via Hyperspectral Remote Sensing and Machine Learning: A Case Study in Jianghuai Hilly Area
Authors: Pan Banglong , Cai Shutong , Zhao Minle , Cheng Hongwei , Yu Hanming , Du Shuhua , Du Juan , Xie Fazhi
Published: August 2023

Soil reflectance spectra and hyperspectral images have great potential to monitor and evaluate soil texture in large-scale scenarios. In hilly areas, sand, clay, and silt have similar spectral characteristics in visible, near-infrared, and short-wave infrared (VNIR-SWIR) reflection spectra. Soil texture spectra belong to mixed spectra despite some differences in particle size, mineral composition, and water content, making their distinction difficult. The accurate identification of the content within different particle sizes is difficult as it involves capturing spectral reflection features. Therefore, this study aimed to predict soil texture content through machine learning and unmixing the soil texture’s spectra while also comparing their respective modelling performances. Taking typical cultivated land in the Jianghuai hills as an example, the GaoFen-5 Advanced Hyperspectral Imaging (GF-5 AHSI) laboratory spectra of soil samples were used to predict sand, silt, and clay particle contents using partial least squares regression (PLSR) and convolutional neural networks (CNNs). The entire spectra of VNIR-SWIR regions were smoothed, and the dimensions were reduced via principal component analysis (PCA). The prediction models of sand, silt, and clay particle content were constructed, and inversion maps were generated using AHSI. The results showed that the PCA-CNN model achieved a higher prediction precision than the PCA-PLSR in both ASD and GF-5 data. Clay content exhibited the highest predictive performance with a coefficient of determination (R2) of 0.948 and 0.908 and a root mean square error (RMSE) of 26.51 g/kg and 31.24 g/kg, respectively, which represented a 39.0% and 79.8% increase in R2 and a 57% and 57.1% decrease in RMSE compared to that of the PCA-PLSR. This method indicates that the PCA-CNN model can effectively achieve nonlinear interactions between multiple spectral components and better model and fit spectral mixing processes; moreover, it provides an alternative method for investigating the spatial distribution of soil texture.
Application of Machine Vision Technology in Citrus Production
Authors: Peng Kaiqian , Ma Wei , Lu Jinzhu , Tian Zhiwei , Yang Zhiming
Published: August 2023

The construction of standardized citrus orchards is the main trend in the future development of modern agriculture worldwide. As the most widely used and mature technology in the agricultural field, machine vision has greatly promoted the industrial development model of the citrus industry. This paper summarizes the application of machine vision technology including citrus pest and disease detection, harvesting identification and localization, and fruit grading. We compare the advantages and disadvantages of relevant research, and analyze the existing problems and prospects for future research. Due to the complex and changeable in-field environment, robots may experience unpredictable interference in the recognition process, which leads to errors in target fruit localization. The lack of datasets also affects the accuracy and stability of the algorithm. While expanding the dataset, it is necessary to conduct further research on the algorithm. In addition, the existing research focuses on indoor monitoring methods, which are not practical for the changeable outdoors environment. Therefore, realizing the diversity of sample datasets, designing agricultural robots suitable for complex environments, developing high-quality image processing hardware and intelligent parallel algorithms, and increasing dynamic monitoring methods are the future research directions. Although machine vision has certain limitations, it is still a technology with strong potential for development.
Microbial Consortium for Polycyclic Aromatic Hydrocarbons Degradation from Petroleum Hydrocarbon Polluted Soils in Rivers State, Nigeria
Authors: Aruotu Jeremiah Oyovweoghene , Chikere Chioma Blaise , Okafor Chidinma Peace , Edamkue Israel
Published: August 2023

The study investigated the distribution of polycyclic aromatic hydrocarbon (PAH) degraders across two different petroleum hydrocarbon-polluted sites in the Niger Delta, Nigeria, and the ability of the reconstituted indigenous consortium to utilize these PAHs. Microorganisms were isolated after sample enrichment in naphthalene and anthracene, and biosurfactant production was measured using the emulsification index technique. PAH concentrations of approximately 6000 mg/kg and 9000 mg/kg in Tombia and Bodo were higher than the Department of Petroleum Resources (DPR) intervention limit of 40 mg/kg. The pH, soil texture and high conductivity affected microbial distribution significantly. A total of 12 bacteria from the genera Bacillus, Pseudomonas, Micrococcus and 3 fungal isolates (Fusarium, Aspergillus and Penicillium) from the 2 sites were able to utilize naphthalene and/or anthracene as sole carbon source. While the Tombia site had more microorganisms capable of PAH degradation with the redox indicator 2, 6-dichlorophenol indophenol (DCPIP) (10 bacterial and 3 fungal species), two bacterial species from Bodo were able to produce biosurfactant. The findings of this study indicate that indigenous microbes in the polluted sites are catabolically active and could be further stimulated for an effective eco-friendly and green removal of PAHs from oil-polluted soils while combined.
Named Entity Recognition in Power Marketing Domain Based on Whole Word Masking and Dual Feature Extraction
Authors: Chen Yan , Liang Zengfu , Tan Zhixiang , Lin Dezhao
Published: August 2023

With the aim of solving the current problems of low utilization of entity features, multiple meanings of a word, and poor recognition of specialized terms in the Chinese power marketing domain named entity recognition (PMDNER), this study proposes a Chinese power marketing named entity recognition method based on whole word masking and joint extraction of dual features. Firstly, word vectorization of the electricity text data is performed using the RoBERTa pre-training model; then, it is fed into the constructed dual feature extraction neural network (DFENN) to acquire the local and global features of text in a parallel manner and fuse them. The output of the RoBERTa layer is used as the auxiliary classification layer, the output of the DFENN layer is used as the master classification layer, and the output of the two layers is dynamically combined through the attention mechanism to weight the outputs of the two layers so as to fuse new features, which are input into the conditional random field (CRF) layer to obtain the most reasonable label sequence. A focal loss function is used in the training process to alleviate the problem of uneven sample distribution. The experimental results show that the method achieved an F1 value of 88.58% on the constructed named entity recognition dataset in the power marketing domain, which is a significant improvement in performance compared with the existing methods.
Mechanical Response Law and Parameter Influence Analysis of Karst Tunnel Dynamic Excavation
Authors: Han Gang , Xue Penghui , Wang Yanyan , Li Xian , Bian Hanbing , Wang Yixian , Guo Panpan
Published: August 2023

To ensure the stability of the tunnel structure, this paper simulates the excavation process of shield tunneling in karst areas, and monitors the top of the arch, the bottom of the arch and the waist of the arch within the influence of the cave. It obtains the displacement and stress change laws under the influence of the upper cave and the lower cave. Finally, the main factors causing karst collapse in tunnels are explored through orthogonal tests. The results indicate that the displacement of the surrounding rock within the influence of the cave decreases and the stress increases. When the cavity and the tunnel reach the safety limit distance, the effect of the lower cavity on the stress around the tunnel is more obvious than that of the upper cavity. The results of the orthogonal test show that when the cavity is above the tunnel, the tunnel burial depth has the greatest influence on the stability of the surrounding rock; when the cavity is below the tunnel, the cavity height has the greatest influence on the stability of the surrounding rock.
An Experimental Study on Estimation of the Lateral Earth Pressure Coefficient (K) from Shaft Friction Resistance of Model Piles under Axial Load
Authors: Aksoy Huseyin Suha , Taher Nichirvan Ramadhan , Ozpolat Aykut , Gör Mesut , Edan Omer Muhammad
Published: August 2023

Estimating a pile shaft’s frictional capacity is challenging and has been a controversial subject among researchers. In this study, the shaft friction resistance of non-displacement (pre-installed) model piles under axial load was investigated. Four different model piles were used, including steel, timber, and two composite piles (FRP and PVC filled with concrete). The angle of interface friction (δ) between test sand, and pile materials was determined using an interface shear test (IST) at four relative densities. Axial pile load experiments were implemented in a soil tank and piles were embedded into loose to very dense sand. Model pile load tests were performed in such a way that there was no end (point) bearing capacity (only friction was generated), and lateral friction resistance between the pile material and the soil along the pile shaft formed the complete bearing capacity of the model pile. According to experimental results, it was observed that, with increasing sand relative density and surface roughness of the pile material, the shaft friction resistance of the model pile increases. A back-calculation analysis was also performed to find the values of lateral earth pressure coefficient (K) using Burland’s (1973) equation with the help of measured shaft friction capacity of the model pile load test. By performing multivariate regression analysis, an equation was obtained between the back-calculated lateral earth pressure coefficient (K) and other parameters. The obtained equation was used to calculate the K values given in other studies in the literature. It was determined that the obtained equation was in good agreement with the data in other studies. This equation can be beneficial in practice and can be advantageous for further study in the future.
Spatiotemporal Analysis of Urban Carbon Metabolism and Its Response to Land Use Change: A Case Study of Beijing, China
Authors: Hu Yingjie , Sun Jin , Zheng Ji
Published: August 2023

Analyzing the spatial pattern of urban carbon metabolism could provide insights into spatial adjustments to mitigate the greenhouse effect. Using CASA and empirical coefficients, we quantitatively analyzed and mapped the spatial pattern of the urban carbon metabolism of Beijing and its response to land use change from 2000 to 2020. The results showed that the carbon emission rate of Beijing increased in the first decade and decreased in the next, while the carbon sequestration rate kept rising over the past two decades. The net carbon emission rate of Beijing averaged 1284.52 × 107 kg C yr−1, indicating that the city functioned as a net carbon source throughout the study period. The most harmful carbon transitions were always sourced from the southeastern suburban area, where the natural components were converted to artificial components, while beneficial carbon transitions were in the urban central area, where the artificial component with a higher carbon emission density was converted to the other types of artificial components with relatively a lower carbon emission density, and the northwestern mountainous areas, where land use types transferred out of and into the forest or grass. The spatiotemporal change in urban carbon metabolism was highly correlated with the land use transition, and the land use change from cultivated land to industrial land accounted for 34.87% of the harmful carbon transitions. These results of key carbon flows and hotspots provide insights for policymaking in the effective management of reducing carbon emissions and enhancing carbon sequestration.
Differences in Outdoor Thermal Comfort between Local and Non-Local Tourists in Winter in Tourist Attractions in a City in a Severely Cold Region
Authors: Liu Zheming , Xu Weiqing , Hu Chenxin , Zhao Caiyi , Yang Tong , Xi Tianyu , Wang Qiaochu
Published: August 2023

The unique climate and the landscape of severely cold regions in winter attract many tourists. The outdoor thermal environment affects the space use and the tourist experience, becoming one of the key factors in the design of tourist attractions. The outdoor thermal comfort of tourists from different regions should be considered, but it has been poorly studied in winter in severely cold regions. This paper explores the differences in outdoor thermal comfort in winter between local and non-local tourists through the field measurement of the thermal environment and a questionnaire survey of thermal comfort at tourist attractions in Harbin, China. The results show that the proportion of local tourists who expect the air temperature and solar radiation to rise in winter is higher than that of non-local tourists. The thermal sensation vote of local tourists is generally higher than that of non-local tourists. When the Physiologically Equivalent Temperature (PET) < −6 °C, the thermal satisfaction of non-local tourists is higher than that of local tourists. When the PET value is −10 °C, the thermal comfort of non-local tourists is the highest. The thermal comfort decreases with the rise or fall of the PET value. When −28 °C < PET < −7 °C, the thermal comfort of non-local tourists is generally higher than that of local tourists. This paper provides a reference and evaluation basis for urban tourist attractions’ outdoor thermal environment design in severely cold regions.
Chitosan Sponges for Efficient Accumulation and Controlled Release of C-Phycocyanin
Authors: Sergeeva Yana E. , Zakharevich Anastasia A. , Sukhinov Daniil V. , Koshkalda Alexandra I. , Kryukova Mariya V. , Malakhov Sergey N. , Antipova Christina G. , Klein Olga I. , Gotovtsev Pavel M. , Grigoriev Timofei E.
Published: August 2023

The paper proposed a new porous material for wound healing based on chitosan and C-phycocyanin (C-PC). In this work, C-PC was extracted from the cyanobacteria Arthrospira platensis biomass and purified through ammonium sulfate precipitation. The obtained C-PC with a purity index (PI) of 3.36 ± 0.24 was loaded into a chitosan sponge from aqueous solutions of various concentrations (250, 500, and 1000 mg/L). According to the FTIR study, chitosan did not form new bonds with C-PC, but acted as a carrier. The encapsulation efficiency value exceeded 90%, and the maximum loading capacity was 172.67 ± 0.47 mg/g. The release of C-PC from the polymer matrix into the saline medium was estimated, and it was found 50% of C-PC was released in the first hour and the maximum concentration was reached in 5–7 h after the sponge immersion. The PI of the released C-PC was 3.79 and 4.43 depending on the concentration of the initial solution.
Immunotherapy in Acute Leukemias: Past Success Paves the Way for Future Progress
Authors: Chergui Adel , Reagan John L.
Published: August 2023

Immunotherapy as a cancer treatment modality has undergone recent widespread proliferation across all cancer types, especially amongst patients with solid tumors. However, the longest tenured immunotherapy approach to cancer is allogeneic stem cell transplantation (allo-SCT) for two hematologic malignancies: acute myeloid and acute lymphoid leukemia (AML and ALL, respectively). While allo-SCT remains a standard of care for eligible patients, recent advances/applications of monoclonal antibodies, immune checkpoint inhibitors, bispecific T-cell engagers (BiTEs), and CAR T-cell therapy are changing the treatment landscape for these acute leukemias by either direct to tumor immune targeting or through decreased toxicities that expand patient eligibility. Pre-clinical data and clinical trials have shown promising results for novel immunotherapies in acute leukemia, and multiple ongoing trials are investigating these novel approaches. While there have been promising results with these approaches, particularly in the relapsed/refractory setting, there remain challenges in optimizing the use of these therapies, such as managing cytokine release syndrome and other immune-related toxicities. Immunotherapy is a rapidly evolving field in the treatment of acute leukemia and has the potential to significantly impact the management of both AML and ALL. This review highlights the history of immunotherapy in the treatment of acute leukemias, the evolution of immunotherapy into more targeted approaches, the potential benefits and limitations of different immune targeting approaches, and ongoing research and development in the field.
Proteomic Mapping of the Interactome of KRAS Mutants Identifies New Features of RAS Signalling Networks and the Mechanism of Action of Sotorasib
Authors: Nolan Aoife , Raso Cinzia , Kolch Walter , Kriegsheim Alex von , Wynne Kieran , Matallanas David
Published: August 2023

RAS proteins are key regulators of cell signalling and control different cell functions including cell proliferation, differentiation, and cell death. Point mutations in the genes of this family are common, particularly in KRAS. These mutations were thought to cause the constitutive activation of KRAS, but recent findings showed that some mutants can cycle between active and inactive states. This observation, together with the development of covalent KRASG12C inhibitors, has led to the arrival of KRAS inhibitors in the clinic. However, most patients develop resistance to these targeted therapies, and we lack effective treatments for other KRAS mutants. To accelerate the development of RAS targeting therapies, we need to fully characterise the molecular mechanisms governing KRAS signalling networks and determine what differentiates the signalling downstream of the KRAS mutants. Here we have used affinity purification mass-spectrometry proteomics to characterise the interactome of KRAS wild-type and three KRAS mutants. Bioinformatic analysis associated with experimental validation allows us to map the signalling network mediated by the different KRAS proteins. Using this approach, we characterised how the interactome of KRAS wild-type and mutants is regulated by the clinically approved KRASG12C inhibitor Sotorasib. In addition, we identified novel crosstalks between KRAS and its effector pathways including the AKT and JAK-STAT signalling modules.
Predictors of Testicular Cancer Mortality in Brazil: A 20-Year Ecological Study
Authors: Franco Ana Paula de Souza , Lima Figueiredo Eric Renato , Melo Giovana Salomão , Souza Josiel de Souza e , Gonçalves Nelson Veiga , Gomes Fabiana de Campos , Neto João Simão de Melo
Published: August 2023

Testicular cancer is common in young men, and early detection and multimodality treatment can lead to successful outcomes. This study aims to identify sociodemographic and risk factors associated with higher testicular cancer mortality and poorer survival rates, while examining the impact of diagnostic and treatment procedures on reducing mortality. The retrospective ecological study analyzed mortality data from testicular cancer in Brazil from 2001 to 2020. Sociodemographic variables such as marital status, age, birth period, year of death (cohort), race, and geographic region were assessed. Risk factors included cryptorchidism and pesticide exposure. Data were subjected to statistical analysis, which revealed an increasing trend in mortality after 2011 among persons born after 1976 in the 15–40 age group. Individuals in the South Region, whites, and singles had higher age-standardized mortality rates (ASMRs), while singles had lower survival rates. The Northeast region had a higher survival rate. Fungicides and insecticides increase ASMR in Brazil. Herbicides increase ASMR in the Northeast and Midwest regions and insecticides increase ASMR in the Northeast, Southeast, and Midwest regions. High rates of implementation of diagnostic procedures in the Midwest were not sufficient to reduce ASMR. No treatment procedure was associated with mortality at the national or regional level.
Cardiologist-Performed Baseline Evaluation with an Assessment of Coronary Status for Prostate Cancer Patients Undergoing Androgen Deprivation Therapy: Impact on Newly Diagnosed Coronary Artery Disease
Authors: Rogé Maximilien , Guimas Valentine , Rio Emmanuel , Vaugier Loïg , Perennec Tanguy , Alexandre Joachim , Supiot Stéphane , Martin Mervoyer Elvire
Published: August 2023

Background: Given the potential cardiovascular risks of androgen deprivation therapy (ADT), it is essential to identify patients who may be at an increased risk for coronary artery disease (CAD). Despite the recent ESC recommendations, there is no consensus on when to refer a patient to a cardiologist for further evaluation. Objective: To report on new diagnoses of CAD in patients with prostate cancer (PCa) requiring ADT who underwent a systematic cardio-onco evaluation with an assessment of their coronary status. Design, Setting, and Participants: This is a retrospective, monocentric study that included patients with PCa who had completed a cardio-onco evaluation with an assessment of their coronary status in the cardio-oncology department at the Western Cancer Institute, Nantes, between January 2019 and August 2022. Intervention: The baseline cardio-onco evaluation included a physical exam, transthoracic echography, and electrocardiogram, followed with a systematic evaluation of their coronary status. Outcome Measurements and Statistical Analysis: The primary objective was to determine the incidence of newly diagnosed CAD. The secondary objective was to evaluate the number of changes in cardiovascular treatment. Results and Limitations: Among the 34 patients who underwent cardio-onco evaluation, 7 (20.6%) were diagnosed with CAD, with a median time to diagnosis of 5 months. Most patients were asymptomatic, with one who experienced a myocardial infarction. Of the 27 patients without CAD, 44.4% underwent a therapeutic intervention by the cardiologist, with no cardiac deaths during follow-up. Overall, 55.9% of patients had a therapeutic intervention after the cardio-onco evaluation. Conclusions: The high incidence of newly diagnosed CAD in asymptomatic patients supports the need for screening for CAD in this population. Further research is needed to determine whether routine screening for CAD in patients receiving ADT would result in significant clinical benefits.
Cell Mutagenic Autopolyploidy Enhances Salinity Stress Tolerance in Leguminous Crops
Authors: Mangena Phetole
Published: August 2023

Salinity stress affects plant growth and development by causing osmotic stress and nutrient imbalances through excess Na+, K+, and Cl− ion accumulations that induce toxic effects during germination, seedling development, vegetative growth, flowering, and fruit set. However, the effects of salt stress on growth and development processes, especially in polyploidized leguminous plants, remain unexplored and scantly reported compared to their diploid counterparts. This paper discusses the physiological and molecular response of legumes towards salinity stress-based osmotic and ionic imbalances in plant cells. A multigenic response involving various compatible solutes, osmolytes, ROS, polyamines, and antioxidant activity, together with genes encoding proteins involved in the signal transduction, regulation, and response mechanisms to this stress, were identified and discussed. This discussion reaffirms polyploidization as the driving force in plant evolution and adaptation to environmental stress constraints such as drought, feverish temperatures, and, in particular, salt stress. As a result, thorough physiological and molecular elucidation of the role of gene duplication through induced autopolyploidization and possible mechanisms regulating salinity stress tolerance in grain legumes must be further studied.
The Role of Hedgehog Signaling Pathway in Head and Neck Squamous Cell Carcinoma
Authors: Cierpikowski Piotr , Leszczyszyn Anna , Bar Julia
Published: August 2023

Head and neck squamous cell carcinoma (HNSCC) is the sixth leading malignancy worldwide, with a poor prognosis and limited treatment options. Molecularly targeted therapies for HNSCC are still lacking. However, recent reports provide novel insights about many molecular alterations in HNSCC that may be useful in future therapies. Therefore, it is necessary to identify new biomarkers that may provide a better prediction of the disease and promising targets for personalized therapy. The poor response of HNSCC to therapy is attributed to a small population of tumor cells called cancer stem cells (CSCs). Growing evidence indicates that the Hedgehog (HH) signaling pathway plays a crucial role in the development and maintenance of head and neck tissues. The HH pathway is normally involved in embryogenesis, stem cell renewal, and tissue regeneration. However, abnormal activation of the HH pathway is also associated with carcinogenesis and CSC regulation. Overactivation of the HH pathway was observed in several tumors, including basal cell carcinoma, that are successfully treated with HH inhibitors. However, clinical studies about HH pathways in HNSCC are still rare. In this review, we summarize the current knowledge and recent advances regarding the HH pathway in HNSCC and discuss its possible implications for prognosis and future therapy.
From Youthful Vigor to Aging Decline: Unravelling the Intrinsic and Extrinsic Determinants of Hippocampal Neural Stem Cell Aging
Authors: Jiménez Peinado Patricia , Urbach Anja
Published: August 2023

Since Joseph Altman published his pioneering work demonstrating neurogenesis in the hippocampus of adult rats, the number of publications in this field increased exponentially. Today, we know that the adult hippocampus harbors a pool of adult neural stem cells (NSCs) that are the source of life-long neurogenesis and plasticity. The functions of these NSCs are regulated by extrinsic cues arising from neighboring cells and the systemic environment. However, this tight regulation is subject to imbalance with age, resulting in a decline in adult NSCs and neurogenesis, which contributes to the progressive deterioration of hippocampus-related cognitive functions. Despite extensive investigation, the mechanisms underlying this age-related decline in neurogenesis are only incompletely understood, but appear to include an increase in NSC quiescence, changes in differentiation patterns, and NSC exhaustion. In this review, we summarize recent work that has improved our knowledge of hippocampal NSC aging, focusing on NSC-intrinsic mechanisms as well as cellular and molecular changes in the niche and systemic environment that might be involved in the age-related decline in NSC functions. Additionally, we identify future directions that may advance our understanding of NSC aging and the concomitant loss of hippocampal neurogenesis and plasticity.
DNA Nanotechnology-Empowered Fluorescence Imaging of APE1 Activity
Authors: He Hui , Liu Xiaojun , Wu Yuchen , Qi Lanlin , Huang Jin , Zhou Yan , Zeng Jiahao , Wang Kemin , He Xiaoxiao
Published: August 2023

Apurinic/apyrimidinic endonuclease 1 (APE1), also known as redox factor-1 (Ref-1), is a multifunctional protein that exists widely in living organisms. It can specifically recognize and cleave the DNA in apurinic/apyrimidinic (AP) sites in the base excision repair (BER) pathway, as well as regulate the expression of genes to activate some transcription factors. The abnormal expression and disruptions in the biological functions of APE1 are linked to a number of diseases, including inflammation, immunodeficiency, and cancer. Hence, it is extremely desired to monitor the activity of APE1, acquiring a thorough understanding of the healing process of damaged DNA and making clinical diagnoses. Thanks to the advent of DNA nanotechnology, some nanodevices are used to image the activity of APE1 with great sensitivity and simplicity. In this review, we will summarize developments in DNA-nanotechnology-empowered fluorescence imaging in recent years for APE1 activity according to different types of DNA probes, which are classified into linear DNA probes, composite DNA nanomaterials, and three-dimensional (3D) DNA nanostructures. We also highlight the future research directions in the field of APE1 activity imaging.
Rapid Thermal Processing of Kesterite Thin Films
Authors: Ganchev Maxim , Spasova Stanka , Raadik Taavi , Mere Arvo , Altosaar Mare , Mellikov Enn
Published: August 2023

Multinary chalcogenides with Kesterite structure Cu2ZnSn(S,Se)4 (CZTSSe) are a prospective material base for the enhancement of the photovoltaics industry with abundant and environmentally friendly constituents and appropriate electro-physical properties for building highly efficient devices at a low cost with a short energy pay-back time. The actual record efficiency of 13.6%, which was reached recently, is far below the current isostructural chalcopyrite’s solar cells efficiency of near 24%. The main problems for future improvements are the defects in and stability of the Kesterite absorber itself and recombination losses at interfaces at the buffer and back contacts. Here, we present an investigation into the rapid thermal annealing (RTA) of as-electrodeposited thin films of Cu2ZnSnS4 (CZTS). The treatment was carried out in a cold wall tubular reactor in dynamic conditions with variations in the temperature, speed and time of the specific elements of the process. The effect of annealing was investigated by X-ray diffractometry, Raman scattering and Scanning Electron Microscopy (SEM). The phase composition of the films depending on treatment conditions was analyzed, showing that, in a slow, prolonged, high-temperature process, the low-temperature binaries react completely and only Kesterite and ZnS are left. In addition, structural investigations by XRD have shown a gradual decrease in crystallite sizes when the temperature level and duration of the high-temperature segment increases, and respectively increase in the strain due to the formation of the phases in non-equilibrium conditions. However, when the speed of dynamic segments in the process decreases, both the crystallite size and strain of the Kesterite non-monotonically decrease. The grain sizes of Kesterite, presented by SEM investigations, have been shown to increase when the temperature and the duration increase, while the speed decreases, except at higher temperatures of near 750 °C. The set of experiments, following a scrupulous analysis of Raman data, were shown to have the potential to elucidate a way to ensure the fine manipulation of the substitutional Cu/Zn defects in the structure of CZTS thin films, considering the dependences of the ratios of Q = I287/I303 and Q′ = I338/(I366 + I374) on the process variables. Qualitatively, it can be concluded that increases in the speed, duration and temperature of RTA lead to increases in the order of the structure, whereas, at higher temperatures of near 750 °C, these factors decrease.
Controlling Bowing and Narrowing in SiO2 Contact-Hole Etch Profiles Using Heptafluoropropyl Methyl Ether as an Etchant with Low Global Warming Potential
Authors: You Sanghyun , Yang Hyun Seok , Jeon Dongjun , Chae Heeyeop , Kim Chang-Koo
Published: August 2023

Heptafluoropropyl methyl ether (HFE-347mcc3), as a lower-GWP (global warming potential) alternative to PFCs (perfluorocarbons), was used to etch SiO2 contact holes. The etch profiles of the SiO2 contact holes in HFE-347mcc3/O2/Ar plasmas showed more bowing at lower flow rate ratios of HFE-347mcc3 to Ar, whereas more narrowing occurred at higher ratios. The measurements of the angular dependences of the deposition rates of fluorocarbon films on the surface of SiO2 and the etch rates of SiO2 showed that the shape evolution of contact-hole etch profiles at different HFE-347mcc3/Ar ratios was attributed to an increase in etch resistance and a decrease in etch ability of the sidewalls of the contact hole with the increasing HFE-347mcc3/Ar ratio. This resulted in determining the optimum ratio of HFE-347mcc3 to Ar to achieve the maximum anisotropy of the contact hole etched in HFE-347mcc3/O2/Ar plasmas. By carefully selecting the specific flow rates of HFE-347mcc3/O2/Ar (9/2/19 sccm), a highly anisotropic and bowing-free SiO2 contact hole, with a 100 nm diameter and an aspect ratio of 24, was successfully achieved.
Formation of Tesseract Time Crystals on a Quantum Computer
Authors: Sims Christopher
Published: August 2023

The engineering of new states of matter through Floquet driving has revolutionized the field of condensed matter physics. This technique enables the creation of hybrid topological states and ordered phases that are absent in normal systems. Crystalline structures, exemplifying spatially ordered systems under periodic driving, have been extensively studied. However, recent focus has shifted towards discrete time crystals (DTCs), periodically driven quantum many-body systems that break time translation symmetry under specific conditions. In this paper, the model of discrete time crystals is extended to allow for the formation of time-varying tesseracts, allowing for the investigation of time translational symmetry in pseudo-higher-dimensional lattice systems.
Zn(II) Metal–Organic Frameworks with a Long Spacer Ligand and a Tricarboxylate Coligand
Authors: Lee Dong Hee , Park In-Hyeok
Published: August 2023

The preparations and structural characteristics of three-dimensional Zn(II) metal-organic frameworks (MOFs) with dipyridyl-olefin and tricarboxylate are reported. The solvothermal reactions of zinc(II) nitrate hexahydrate, 1,4-bis [2-(4-pyridyl)ethenyl]benzene (bpeb), and 4,4′,4″,-benzene-1,3,5-triyl-tris(benzoic acid) (H3btb) furnished three Zn(II) MOFs (1–3) with new topologies. Depending on the temperature or mole-ratio variations, self-interpenetrated [Zn2(bpeb)(btb)(OH)]·DMF·H2O (1), noninterpenetrated [Zn3(btb)2(bpeb)]·xSolvent (2), and fourfold interpenetrated [Zn2(Hbtb)2(bpeb)][Zn2(Hbtb)2(bpeb)][Zn4(Hbtb)4(bpeb)2] (3) structures were generated with different molecular building blocks. It is interesting that although all three MOFs contain the same metal cation, anion, and spacer ligand, they show different emissions due to structure and connectivity.
Raman Spectroscopy of 2D MoS2 Interacting with Metals
Authors: Tumino Francesco , D’Agosta Paolo , Russo Valeria , Li Bassi Andrea , Casari Carlo Spartaco
Published: August 2023

The research on molybdenum disulphide (MoS2) has progressed remarkably in the last decade, prompted by the increasing interest for this material as a potential candidate in future ultrathin optoelectronic devices. MoS2 is a layered semiconductor with a gap in the visible region, which can be exfoliated down to the mono-layer form. Since the discovery of the exceptional optoelectronic properties of 2D MoS2, Raman spectroscopy has been extensively used as a tool to characterize the structure and thickness of MoS2 films. Recent works on MoS2-metal interfaces have shown that Raman spectra are significantly affected by the interaction with metals. However, a complete understanding of how such interaction modifies the MoS2 vibrational properties is still lacking. Studying this subject with both experimental and theoretical methods will provide fundamental insight into the interface physics of MoS2-metal systems, which is crucial for the fabrication of metal contacts and for the development of metal-assisted synthesis methods. This review summarizes the main results concerning Raman spectroscopy studies of heterosystems between MoS2 and transition metals, providing both a basis and directions for future research.
Ultra-High-Frequency Ultrasonography of Labial Glands in Pediatric Sjögren’s Disease: A Preliminary Study
Authors: Marrani Edoardo , Fulvio Giovanni , Virgili Camilla , Izzetti Rossana , Dini Valentina , Oranges Teresa , Baldini Chiara , Simonini Gabriele
Published: August 2023

Sjögren’s disease (SD) is a chronic autoimmune disease primarily affecting lacrimal and salivary glands. The diagnosis of pediatric SD mostly relies on clinical suspect, resulting in a significant diagnostic delay. Recently, ultrahigh-frequency ultrasound (UHFUS) of labial glands has been proposed as a diagnostic method in adults with suspected SD. Until now, there have been no studies about UHFUS in pediatric diagnostic work-up. The aim of the study was to evaluate the potential role of UHFUS of minor salivary glands in pediatric SD. Consecutive pediatric patients with a diagnosis of pediatric SD seen at AOU Meyer IRCSS were evaluated. Intraoral UHFUS scan of the lip mucosa was performed with Vevo MD equipment, using a 70 MHz probe with a standardized protocol and the images were independently reviewed by two operators. Lip salivary glands were assessed by using a four-grade semiquantitative scoring system for parenchymal alteration and vascularization. Twelve patients were included. When applying UHFUS to this cohort of patients, all patients showed a UHFUS grade of ≥1 with 8/12 showing a mild glandular alteration (i.e., grade 1), 2/12 a moderate glandular alteration (i.e., grade 2) and finally 2/12 a severe glandular alteration (i.e., grade 3). Moderate intraglandular vascularization was seen in 9/12, with only 3/12 showing mild intraglandular vascularization. Due to limited size of the sample, the relationship between histological findings, autoantibodies status and UHFUS grade could not be performed. This preliminary study seems to report UHFUS as feasibility technique to identify salivary gland alterations in children with a clinical suspect of SD.
How to Imagine a New Community from Science Fiction: A Pedagogical Dramaturgy of Silence, for a Slow Education
Authors: Novoa Andrés González , Méndez Pedro Perera
Published: August 2023

Europe has just established the first regulation for artificial intelligences. Large technology corporations and private educational institutions are already imagining neural networks educating us. Has anyone stopped to think about who, how and for what purpose we humans are going to educate machines? The Spanish critical pedagogy research team (PEDACRI), after participating in international conferences on digital education, robotics, ethics in the metaverse and cartography of hyperreality and participating in various publications on the challenges of pedagogy and ethics in the technologisation of educational processes, reflects in this essay on the challenges and questions we need to ask ourselves to imagine the post-human or trans-human community to come. Reviewing works coming from philosophy and those plays, series and films that address the future and the relationship between humans and machines, we analyse the opportunities and threats that can humanise machines or programme them as soulless weapons, which can civilise us or return us to a state of barbarism. The word robot, let us not forget, is derived from the Polish word roboca, which means “slave”. Will we be able, as the replicant in Blade Runner wonders, to programme silence? What can philosophy and pedagogy contribute to the ethical programming of algorithms?