bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on Swarm Control Based on Complementary Collaboration of Unmanned Aerial Vehicle Swarms Under Complex Conditions
Authors: Zhao Longqian , Chen Bing , Hu Feng
Published: February 2025

Under complex conditions, the collaborative control capability of UAV swarms is considered to be the key to ensuring the stability and safety of swarm flights. However, in complex environments such as forest firefighting, traditional swarm control methods struggle to meet the differentiated needs of UAVs with differences in behavior characteristics and mutually coupled constraints, which gives rise to the problem that adjustments and feedback to the control policy during training are prone to erroneous judgments, leading to decision-making dissonance. This study proposed a swarm control method for complementary collaboration of UAVs under complex conditions. The method first generates training data through the interaction between UAV swarms and the environment; then it captures the potential patterns of UAV behaviors, extracts their differentiated behavior characteristics, and explores diversified behavior combination scenarios with complementary advantages; accordingly, dynamic behavior allocations are made according to the differences in perception accuracy and action capability to achieve collaborative cooperation; and finally, it optimizes the neural network parameters through behavior learning to improve the decision-making policy. According to the experimental results, the UAV swarm control method proposed in this study demonstrates high formation stability and integrity when dealing with the collaborative missions of multiple types of UAVs.
Adversarial Attacks on Supervised Energy-Based Anomaly Detection in Clean Water Systems
Authors: Moradpoor Naghmeh , Abah Ezra , Robles-Durazno Andres , Maglaras Leandros
Published: February 2025

Critical National Infrastructure includes large networks such as telecommunications, transportation, health services, police, nuclear power plants, and utilities like clean water, gas, and electricity. The protection of these infrastructures is crucial, as nations depend on their operation and stability. However, cyberattacks on such systems appear to be increasing in both frequency and severity. Various machine learning approaches have been employed for anomaly detection in Critical National Infrastructure, given their success in identifying both known and unknown attacks with high accuracy. Nevertheless, these systems are vulnerable to adversarial attacks. Hackers can manipulate the system and deceive the models, causing them to misclassify malicious events as benign, and vice versa. This paper evaluates the robustness of traditional machine learning techniques, such as Support Vector Machines (SVMs) and Logistic Regression (LR), as well as Artificial Neural Network (ANN) algorithms against adversarial attacks, using a novel dataset captured from a model of a clean water treatment system. Our methodology includes four attack categories: random label flipping, targeted label flipping, the Fast Gradient Sign Method (FGSM), and Jacobian-based Saliency Map Attack (JSMA). Our results show that, while some machine learning algorithms are more robust to adversarial attacks than others, a hacker can manipulate the dataset using these attack categories to disturb the machine learning-based anomaly detection system, allowing the attack to evade detection.
Rheological Properties of Crude Oil and Produced Emulsion from CO2 Flooding
Authors: Qiao Mingzheng , Zhang Fan , Li Weiqi
Published: February 2025

Carbon Capture, Utilization and Storage (CCUS) technology is recognized as a pivotal strategy to mitigate global climate change. The CO2 storage and enhanced oil recovery (CCUS-EOR) technology not only enhances oil recovery rates but also contributes to significant reductions in CO2 emissions, with significant social and economic benefits. This paper examines the application of CO2-EOR technology in both enhancing oil recovery and facilitating geological CO2 storage, and analyzes its implementation status and differences in the United States and China. Through experimental investigations conducted in a specific oilfield, we analyze the effects of dissolved CO2 on the viscosity–temperature characteristics, yield value under pressure, stability, and rheological properties of crude oil and produced fluids. Additionally, we assess the demulsification effectiveness of various demulsifiers. Our findings indicate that both dissolved CO2 in crude oil and emulsions exhibit non-Newtonian fluid behavior characterized by shear thinning, and the viscosity decreases with the increase in temperature and pressure. Furthermore, the presence of dissolved CO2 exacerbates the oil–water separation phenomenon in produced fluids, thereby diminishing emulsion stability. The increase in emulsion concentration and the increase in emulsification temperature are both conducive to improving the emulsification rate. These research results provide critical insights for pipeline design and pump selection in oilfield production processes.
Underground Hydrogen Storage: Transforming Subsurface Science into Sustainable Energy Solutions
Authors: Opoku Duartey Kwamena , Ampomah William , Rahnema Hamid , Mehana Mohamed
Published: February 2025

As the global economy moves toward net-zero carbon emissions, large-scale energy storage becomes essential to tackle the seasonal nature of renewable sources. Underground hydrogen storage (UHS) offers a feasible solution by allowing surplus renewable energy to be transformed into hydrogen and stored in deep geological formations such as aquifers, salt caverns, or depleted reservoirs, making it available for use on demand. This study thoroughly evaluates UHS concepts, procedures, and challenges. This paper analyzes the most recent breakthroughs in UHS technology and identifies special conditions needed for its successful application, including site selection guidelines, technical and geological factors, and the significance of storage characteristics. The integrity of wells and caprock, which is important for safe and efficient storage, can be affected by the operating dynamics of the hydrogen cycle, notably the fluctuations in pressure and stress within storage formations. To evaluate its potential for broader adoption, we also examined economic elements such as cost-effectiveness and the technical practicality of large-scale storage. We also reviewed current UHS efforts and identified key knowledge gaps, primarily in the areas of hydrogen–rock interactions, geochemistry, gas migration control, microbial activities, and geomechanical stability. Resolving these technological challenges, regulatory frameworks, and environmental sustainability are essential to UHS’s long-term and extensive integration into the energy industry. This article provides a roadmap for UHS research and development, emphasizing the need for further research to fully realize the technology’s promise as a pillar of the hydrogen economy.
Advancing Human Health Risk Assessment Through a Stochastic Methodology for Mobile Source Air Toxics
Authors: Munshed Mohammad , Van Griensven Thé Jesse , Fraser Roydon
Published: February 2025

Mobile source air toxics (MSATs) are major contributors to urban air pollution, especially near high-traffic roadways, where populations face elevated pollutant exposures. Traditional human health risk assessments, based on deterministic methods, often overlook variability in exposure and the vulnerabilities of sensitive subpopulations. This study introduces and applies a new stochastic modeling approach, utilizing Monte Carlo simulations to evaluate cumulative cancer risks from MSATs exposure through inhalation and ingestion pathways. This method captures variability in exposure scenarios, providing detailed health risk assessments, particularly for vulnerable groups such as children and the elderly. This approach was demonstrated in a case study conducted in Saint Paul, Minnesota, using 2019 traffic data. Deterministic models estimated cumulative cancer risks for adults at 6.24E-02 (unitless lifetime cancer risk), while stochastic modeling revealed a broader range, with the 95th percentile reaching 4.98E-02. The 95th percentile, used in regulatory evaluations, identifies high-risk scenarios overlooked by deterministic methods. This research advances the understanding of MSATs exposure risks by integrating spatiotemporal dynamics, identifying high-risk zones and vulnerable subpopulations, and supporting resource allocation for targeted pollution control measures. Future applications of this methodology include expanding stochastic modeling to evaluate ecological risks from mobile emissions.
Storage Quality Improvement of Duck Breast Meat: Role of Ultrasound-Assisted Slightly Acidic Electrolyzed Water
Authors: Xu Anqi , Zhang Siyi , Huan Chuanming , Gao Sumin , Wang Hengpeng , Gao Ziwu , Wu Ruiyun , Wang Zhenyu , Meng Xiangren
Published: February 2025

This study evaluated the effects of ultrasound-assisted slightly acidic electrolyzed water (SAEW) treatment on duck breast meat storage quality. The impact of different treatments—ultrasound combined with SAEW, SAEW alone, ultrasound combined with water, and water-alone treatment—on the freshness, texture, protein oxidation, and microbiological diversity of the meat was assessed under vacuum packaging at 4 °C. The results demonstrated that the ultrasound–SAEW combination significantly slowed pH decline, inhibited total volatile basic nitrogen (TVB-N) formation, and preserved the redness (a*) and texture of duck breast meat. Additionally, carbonyl and total sulfhydryl measurements indicated that the combined treatment delayed protein oxidation. 16S rDNA analysis showed that combined treatment reduced the microbial abundance, particularly Pseudomonas and Candida. After 9 days of storage, the total viable count (TVC) of the treated duck breast meat remained within the GB 16869—2005 microbial contamination threshold for fresh meat (5.56 log10 CFU/g). These findings highlight the effectiveness of ultrasound-assisted SAEW in extending the shelf life and maintaining the quality of duck breast meat.
Construction of a ‘Simple, Fast and Accurate’ Evaluation Method for Profile Control and Plugging Effect of Gel Plugging Agent Based on Simulations
Authors: Wang Zengbao , Jiang Junjie , Huang Weian , Gan Yuwei , Bai Yingrui
Published: February 2025

At present, the evaluation perspective of the gel plugging agent assessment method is incomprehensive, due to which the experimental results deviate from the field data. By analyzing the current indoor evaluation methods and the factors controlling the sealing capability of gel plugging agents, an experimental device and method for evaluating the blocking effect of oilfield gel plugging agents has been designed. In contrast to traditional assessment methods, the proposed approach offers advantages such as simple operation, rapid experimentation, and accurate results. The experimental results show that gels selected using conventional methods are inconsistent with the results of plugging displacement tests. This discrepancy can be attributed to the fact that these methods focus solely on cohesive strength while neglecting adhesive strength. Considering that the evaluation perspective of conventional methods is relatively limited, an evaluation method for the sealing effect of the plugging agent was developed. This method comprehensively incorporates factors such as cohesion strength, adhesion capability, shear resistance, and the long-term anti-dehydration performance of the gel. The evaluation results of the method were consistent with the results of the plugging displacement experiments. The newly constructed method defines Γ as the comprehensive evaluation parameter for the gel. A new experimental system with a comprehensive evaluation index (Γ) of 8.97 Pa2 was selected. After the profile control of the system, the effluent ratio of the high and low permeability layers reached 1:9, and its erosion resistance was greater than 20 PV. Meanwhile, the profile control effect was also stable. Through verification based on field data, the injection pressure of the system optimized by the proposed method was found to be 2.5 times higher than that of the original system. Meanwhile, the plugging validity period was >2 times of the original system. The test results were consistent with the plugging capability evaluation index. In summary, the performance evaluation method of the designed gel plugging agent was reasonable in principle and the results were accurate and reliable. Therefore, it is considered to be of guiding significance for the selection of efficient profile control plugging agents in oilfields.
A Protocol for Enhancing Allied Health Care for Older People in Residential Care: The EAHOP Intervention
Authors: Isbel Stephen , D’Cunha Nathan M. , Wiseman Lara , Dawda Paresh , Kosari Sam , Pearce Claire , Fearon Angela , Sabeti Faran , Hewitt Jennifer , Kellett Jane , Naunton Mark , Southwood Helen , Logan Pip , Subramanian Ramanathan , Chadborn Neil H. , Davey Rachel , Bail Kasia , Goss John R. , Ambikairajah Ananthan , Lincoln Michelle , Holloway Helen , Gibson Diane
Published: February 2025

Background: Complications due to frailty and injury after falls are a significant problem for people living in residential aged care. This can lead to a range of negative outcomes including poor physical, social, and psychological well-being. The Australian Royal Commission into Aged Care Quality and Safety highlighted many aspects of care in residential aged care homes requiring improvement, leading to specific recommendations aimed at improving the outcomes in this area. This contributed to four recommendations calling for increased allied health interventions to meet the unmet needs in residential aged care. This intervention aims to implement and evaluate evidence-based allied health interventions for people living in residential aged care specifically relating to frailty, preventing falls, and maintaining engagement in everyday activities. Method: This is a pragmatic, non-randomised, pre–post design study where six groups of up to 10 residents of an aged care home will start the intervention at staggered times. The EAHOP intervention is an integrated application of a suite of allied health services (occupational therapy, physiotherapy, dietetics, speech pathology, pharmacy, and optometry), with general practitioner involvement, using allied health assistants in an integrated transdisciplinary model of care. The baseline period is 6 weeks, and intervention is a maximum of 36 weeks with follow-up at 12 and 24 weeks. Primary outcomes measure changes in falls, frailty, and quality of life. A qualitative program evaluation will be completed as well as an economic analysis. Conclusion: The results of the study will provide information about the clinical, implementation, and effectiveness outcomes of this integrated, transdisciplinary allied health service model for people living in residential aged care. The results will be used to develop evidence-informed guidelines for residential aged care providers on the delivery of allied health services.
Risk and Preventive Measures Among Older Adults in Nursing Homes in Saudi Arabia: An Exploratory Study on Falls
Authors: Aljarbou Hmoud M. , Almoajel Alia M. , Althomali Mohammed M. , Almutairi Khaled M.
Published: February 2025

Background: Falls among older adults are a pervasive and significant concern worldwide. A practice guideline has been developed to address the prevention of falls and their resulting consequences in hospital and long-term care settings. Aim: The study aimed to assess the fall down rate and preventive tools among older adult patients in nursing homes. Methods: A cross-sectional study was conducted on randomly selected older adult patients by using a questionnaire with the Stopping Elderly Accidents, Deaths, and Injuries (STEADI) tool in nursing homes at the Ministry of Human Resource and Social Development. Results: Among 323 older adult patients, most of them (73.1%) were male, 23.8% were from Makkah, and the age ranged from 60 to 90 years and older. Results showed that 64.8% had a psychiatric disorder, 41.8% had hypertension, 38.4% had diabetes, 38.1% had movement disorders, 11.3% had heart diseases, and 1.5% had no chronic conditions. The mean STEADI tool score was 3.5 out of 12, and of the 323 older adult patients, 51.7% had a low risk to fall and 48.3% had a high risk to fall. Of the 13 interventions used to prevent falls, the most used intervention was rehabilitative physical therapy, followed by providing patient facilities and muscle strengthening exercises. Conclusions: The level of falls was markedly low, and a significant correlation was observed between the risk of falling and the participants’ region of residence.
Non-Invasive Investigation of 19th-Century Photographs: Enrico Van Lint’s Historical Collection in Pisa
Authors: Poggialini Francesco , Campanella Beatrice , Giostrella Claudia , Lorenzetti Giulia , Palleschi Vincenzo , Raneri Simona , Legnaioli Stefano
Published: February 2025

Enrico Van Lint (Pisa, 1808–1884) was a very prolific photographer, active in Pisa in the 19th century where he had a prominent photographic atelier. He was a meticulous experimenter, investigating the evolving photographic activity of his historical period. While his early works included calotypes using Fox Talbot’s methods, he rapidly adopted the collodion processes, becoming one of the most important Italian photographers that used this technique. At the present time, a vast number of examples of the works from Van Lint’s ateliers are preserved and archived in Pisa, under the supervision of the Italian Ministry of Culture in the Photographic Archive of the “Soprintendenza Archeologia, Belle Arti e Paesaggio per le provincie di Pisa e Livorno” (SABAP). This collection is composed of positive prints as well as glass plate negatives, from both Van Lint himself and his colleagues. To this day, Van Lint’s collection has not been studied using analytical techniques, and the identification of the photographic processes involved in the preparation of the positive prints has relied exclusively on thorough observation by historians and conservators. This provides a unique occasion for a first study of Van Lint’s collection, using multiple non-invasive and non-destructive techniques (multispectral imaging, XRF, and FTIR) that can identify the photographic process used to make the positives, as well as highlight significant differences or degradation phenomena. In this preliminary work, we investigated a selection of ten positive prints, attributed to both Van Lint himself and later reproductions from the original glass negatives. The selected samples include prints previously classified as albumen prints and gelatin prints, displaying slight differences in conservation status as well as in print finish. This analytical approach allowed for a proper characterization of these Van Lint’s prints, improving the historical and conservation knowledge to implement the best preventive preservation actions in the near future.
Intercropping Between Panax ginseng and Arisaema amurense Improves Ginseng Quality by Improving Soil Properties and Microbial Communities
Authors: Lv Bochen , Sun Hai , Cao Weiyu , Zhu Jiapeng , Liang Hao , Long Hongjie , Cui Yanmei , Shao Cai , Zhang Yayu
Published: February 2025

The imbalance in soil microcosm systems caused by the long-term monoculture of ginseng is the main cause of continuous cropping disorder in ginseng, an important factor limiting the development of the ginseng industry. The ecological intercropping pattern of medicinal plants is a planting technology that achieves efficient, high-quality and sustainable production of Chinese medicinal materials by increasing the diversity of farmland ecosystems and improving the stability of soil micro-ecosystems, thereby alleviating the continuous cropping disorder of medicinal plants. However, there remains a lack of research on the ecological intercropping cultivation of ginseng. We constructed a Panax ginseng/Arisaema amurense intercropping model to explore the changes in soil nutrients, enzyme activities, soil microbial communities and ginseng quality. The findings of this study demonstrated that intercropping could decelerate the acidification process of soils and effectively increased 37.02% of soil organic matter, 32.39% of total nitrogen, 5.18% of total potassium and 9.03% of available phosphorus contents in ginseng inter-root soil compared with monocropping. The results revealed that intercropping increased the soil urease and soil acid phosphatase activities while reducing the soil sucrase activity in the inter-root soil. Additionally, intercropping elevated the α-diversity of the inter-root soil bacterial community and diminished the composition and abundance of the fungal community. The intercropping exhibited a pronounced inhibitory impact on two common genera of pathogenic fungi, Fusarium and Cylindrocarpon Furthermore, the total ginsenosides and diverse monomer ginsenosides present in the roots of intercropped ginseng exhibited varying degrees of enhancement. The results of the analyses indicated that the observed increase in ginsenoside content under intercropping was attributable to interactions between soil microorganisms, including the Prevotella_7, Penicillium, Humicola and Deconica, and soil factors such as SOM, NH4+–N, AP and S-UE. Thus, implementing P. ginseng/A. amurense ecological intercropping can effectively mitigate soil acidification, enhance soil nutrient effectiveness, optimize soil microbial community composition and augment ginsenoside content.
Insights into the Role of GhTAT2 Genes in Tyrosine Metabolism and Drought Stress Tolerance in Cotton
Authors: Mehari Teame Gereziher , Tang Jungfeng , Gu Haijing , Fang Hui , Han Jinlei , Zheng Jie , Liu Fang , Wang Kai , Yao Dengbing , Wang Baohua
Published: February 2025

Gossypium hirsutum is a key fiber crop that is sensitive to environmental factors, particularly drought stress, which can reduce boll size, increase flower shedding, and impair photosynthesis. The aminotransferase (AT) gene is essential for abiotic stress tolerance. A total of 3 Gossypium species were analyzed via genome-wide analysis, and the results unveiled 103 genes in G. hirsutum, 47 in G. arboreum, and 53 in G. raimondii. Phylogenetic analysis, gene structure examination, motif analysis, subcellular localization prediction, and promoter analysis revealed that the GhAT genes can be classified into five main categories and play key roles in abiotic stress tolerance. Using RNA-seq expression and KEGG enrichment analysis of GhTAT2, a coexpression network was established, followed by RT-qPCR analysis to identify hub genes. The RT-qPCR results revealed that the genes Gh_A13G1261, Gh_D13G1562, Gh_D10G1155, Gh_A10G1320, and Gh_D06G1003 were significantly upregulated in the leaf and root samples following drought stress treatment, with Gh_A13G1261 identified as the hub gene. The GhTAT2 genes were considerably enriched for tyrosine, cysteine, methionine, and phenylalanine metabolism and isoquinoline alkaloid, tyrosine, tryptophan, tropane, piperidine, and pyridine alkaloid biosynthesis. Under drought stress, KEGG enrichment analysis manifested significant upregulation of amino acids such as L-DOPA, L-alanine, L-serine, L-homoserine, L-methionine, and L-cysteine, whereas metabolites such as maleic acid, p-coumaric acid, quinic acid, vanillin, and hyoscyamine were significantly downregulated. Silencing the GhTAT2 gene significantly affected the shoot and root fresh weights of the plants compared with those of the wild-type plants under drought conditions. RT-qPCR analysis revealed that GhTAT2 expression in VIGS-treated seedlings was lower than that in both wild-type and positive control plants, indicating that silencing GhTAT2 increases sensitivity to drought stress. In summary, this thorough analysis of the gene family lays the groundwork for a detailed study of the GhTAT2 gene members, with a specific focus on their roles and contributions to drought stress tolerance.
Gender Differences in Adenine Diet-Induced Kidney Toxicity: The Impact of 17β-Estradiol on Renal Inflammation and Fibrosis
Authors: Ha Sugyeong , Son Minjung , Kim Jeongwon , Kim Doyeon , Kim Mi-Jeong , Yoo Jian , Kim Byeong Moo , Kim Donghwan , Chung Hae Young , Chung Ki Wung
Published: February 2025

Chronic kidney disease (CKD) involves ongoing impairment of kidney function and structural changes. Previous studies indicated that males have a substantially higher prevalence of CKD than those observed in females. Here, we compared the gender differences in CKD development by comparing age-matched male and female mice subjected to a 0.25% adenine diet (AD) for two weeks. Male mice showed a significantly greater decrease in kidney function than female mice, as evidenced by the elevated blood urea nitrogen levels (M-AD: 160 ± 5 mg/dL, F-AD: 90 ± 4 mg/dL; p < 0.001). Furthermore, male mice kidneys exhibited pronounced tubule dilation and kidney damage, as detected by histological and biochemical methods. The extent of fibrosis was quantified using multiple biological methods, revealing a greater degree of fibrosis in male kidneys. We next indicated the inflammatory responses in the kidneys. Similar to the extent of fibrosis, AD-fed male mice showed significantly increased levels of pro-inflammatory markers, including cytokine expression and infiltration of immune cell, compared to female mice. Based on in vivo observations, the anti-inflammatory and anti-fibrotic effects of 17β-estradiol (E2) were further evaluated in vitro conditions. E2 pre-treatment significantly reduced lipopolysaccharide-induced inflammatory response through inhibition of the nuclear factor-kappa B (NF-κB) pathway in NRK52E renal epithelial cells. In NRK49F renal fibroblasts, E2 pre-treatment also reduced TGFβ-induced fibrotic responses. We further demonstrated that E2 markedly decreased fibrosis and inflammation in AD-fed mouse kidneys. Our observations revealed that male mice kidneys exhibited a heightened inflammatory and fibrotic response compared to female mice kidneys. Additionally, our findings suggest that the observed sex differences may be partially attributed to the potential anti-inflammatory and anti-fibrotic effects of E2.
The Loop-In Binding Mode of Dihydroorotase: Implications for Ligand Binding and Therapeutic Targeting
Authors: Huang Cheng-Yang
Published: February 2025

Dihydroorotase (DHOase; EC 3.5.2.3) is a zinc-dependent metalloenzyme that plays a key role in the de novo pyrimidine biosynthesis pathway, catalyzing the reversible cyclization of N-carbamoyl aspartate to dihydroorotate. This reaction is essential for the production of uridine monophosphate, the precursor of all pyrimidine nucleotides required for DNA and RNA synthesis. Despite its conserved enzymatic function, DHOase exhibits significant structural diversity across species, particularly in its oligomeric states, gene fusion patterns, and active site architecture. A crucial structural feature of DHOase is its flexible active site loop, which undergoes dynamic conformational changes during catalysis. Previously, the loop-in conformation was associated with substrate binding, whereas the loop-out conformation was linked to product release and non-substrate ligand binding. However, recent crystallographic studies challenge this paradigm, revealing that certain non-substrate ligands and inhibitors, including malate, 5-fluoroorotate, plumbagin, 5-aminouracil, and 5-fluorouracil, interact with DHOase via a loop-in binding mechanism rather than the previously assumed loop-out mode. These findings necessitate a reassessment of the catalytic mechanism of DHOase and underscore the active site loop as a potential target for drug development. This review revisits the structural and biochemical mechanisms of DHOase, with a focus on recent crystallographic insights that redefine the loop-in binding mode for ligand interaction. By leveraging the unique conformational dynamics of the active site loop, novel inhibitors may be developed to selectively target pyrimidine biosynthesis in cancer cells and microbial pathogens. These insights emphasize the crucial role of structural biology in therapeutic design and highlight DHOase as a promising drug target.
The Silencing of the StPAM16-1 Gene Enhanced the Resistance of Potato Plants to the Phytotoxin Thaxtomin A
Authors: Liu Lu , Song Shuangwei , Liu Ning , Wang Zhiqin , Zhao Yonglong , Zhong Naiqin , Zhao Pan , Wang Haiyun
Published: February 2025

Potato common scab (CS) caused by Streptomyces scabiei is a severe disease that threatens tuber quality and its market value. To date, little is known about the mechanism regulating the resistance of potato to CS. In this study, we identified a presequence translocase-associated motor 16 gene from potato (designated StPAM16-1) that is involved in the response to the phytotoxin thaxtomin A (TA) secreted by S. scabiei. The StPAM16-1 protein was localized in the mitochondria, and the expression of the gene was upregulated in potato leaves treated with TA. The suppression of StPAM16-1 in potato led to enhanced resistance to TA and S. scabiei. Protein interaction analyses revealed that StPAM16-1 interacted with the subunit 5b of the COP9 signalosome complex (StCSN5). Similar to that of StPAM16-1, the expression levels of StCSN5 significantly increased in potato leaves treated with TA. These results indicated that StPAM16-1 acted as a negative regulator and was functionally associated with StCSN5 in the immune response of potato plants against CS. Our study sheds light on the molecular mechanism by which PAM16 participates in the plant immune response. Furthermore, both StPAM16-1 and StCSN5 could be potential target genes in the molecular breeding of potato cultivars with increased resistance to CS.
Tadalafil Ameliorates Chronic Ischemia-Associated Bladder Overactivity in Fructose-Fed Rats by Exerting Pelvic Angiogenesis and Enhancing p-eNOS Expression
Authors: Lee Wei-Chia , Lu Steve , Su Chia-Hao , Tain You-Lin , Wu Kay L. H. , Hsu Chien-Ning , Tzeng Hong-Tai
Published: February 2025

Metabolic syndrome (MetS) can contribute to a chronic ischemia-relative overactive bladder (OAB). Using fructose-fed rats (FFRs), a rat model of MetS, we investigated the effects of tadalafil (a phosphodiesterase-5 inhibitor) on MetS-associated chronic bladder ischemia and bladder overactivity. Phenotypes of the OAB, including increased micturition frequency and a shortened intercontractile interval in cystometry, were observed in FFRs, together with reduced bladder blood perfusion (in empty bladders) via laser color Doppler imaging and elevated serum nitrite levels, suggesting chronic ischemia-related bladder dysfunction. Treatment with tadalafil (2 mg/kg) promoted pelvic angiogenesis, as shown by magnetic resonance imaging, and increased VEGF and p-eNOS overexpression in the bladder. This treatment restored bladder perfusion and alleviated bladder overactivity without significantly altering most MetS parameters. At the molecular level, FFRs exhibited increased ischemia markers (NGF, HIF-2α, and AMPK-α2) and decreased p-AMPK-α2, along with elevated proinflammatory mediators (ICAM-1, nuclear NF-κB, COX-2, IL-1β, IL-6, and TNF-α), enhanced mitochondria biogenesis (PGC-1α, TFAM, and mitochondria DNA copy number), oxidative stress (decreased nuclear NRF2, increase MnSOD and 8-OHdG staining), and tissue fibrosis (increased TGF-β1, collagen I, and fibronectin). Tadalafil treatment improved these effects. Together, these findings suggest that tadalafil may promote VEGF-associated angiogenesis, enhance p-eNOS staining in the bladder vasculature, normalize bladder perfusion in microcirculation, and reduce serum nitrite levels. Consequently, tadalafil mitigates the adverse effects of chronic ischemia/hypoxia, improving bladder overactivity. We elucidated the mechanisms underlying the tadalafil-mediated amelioration of MetS-associated OAB symptoms.
Phylogeny of Camphora and Cinnamomum (Lauraceae) Based on Plastome and Nuclear Ribosomal DNA Data
Authors: Xu Jian , Zhang Haorong , Yang Fan , Zhu Wen , Li Qishao , Cao Zhengying , Song Yu , Xin Peiyao
Published: February 2025

Camphora Fabr. is a genus in the family Lauraceae, comprising over 20 tropical and subtropical tree species. Since the genera Camphora and Cinnamomum Schaeff. were described, there has been a long-lasting controversy regarding the phylogenetic relationships among taxa in both genera. In particular, phylogenetic inferences derived from plastid data remain debated, with varying hypotheses proposed and occasional disputes concerning the monophyly of Camphora taxa. To further investigate the relationships, We analyzed plastomes and nuclear ribosomal cistron sequences (nrDNA) of 22 Camphora taxa, 15 Cinnamomum taxa, and 13 representative taxa of related genera. The Camphora plastomes range from 152,745 to 154,190 bp, with a GC content of 39.1% to 39.2%. A total of 128 genes were identified in the Camphora plastomes, including 84 protein-coding genes, 8 rRNA genes, and 36 tRNA genes. A total of 1130 SSR loci were detected from plastomes of Camphora, and A/T base repeats looked like the most common. Comparative analyses revealed that the plastomes of Camphora exhibit high similarity in overall structure. The loci ycf1, ycf2, trnK (UUU), psbJ-psbL, and ccsA-ndhD were identified as candidate DNA barcodes for these taxa. Plastome phylogenetic analysis revealed that Camphora is not monophyletic, whereas the nrDNA dataset supported the monophyly of Camphora. We propose that intergeneric hybridization may underlie the observed discordance between plastid and nuclear data in Camphora, and we recommend enhanced taxonomic sampling and precise species identification to improve phylogenetic resolution and accuracy.
The Disruptions of Sphingolipid and Sterol Metabolism in the Short Fiber of Ligon-Lintless-1 Mutant Revealed Obesity Impeded Cotton Fiber Elongation and Secondary Cell Wall Deposition
Authors: Tian Huidan , Wang Qiaoling , Yan Xingying , Zhang Hongju , Chen Zheng , Ma Caixia , Meng Qian , Xu Fan , Luo Ming
Published: February 2025

Boosting evidence indicated lipids play important roles in plants. To explore lipid function in cotton fiber development, the lipid composition and content were detected by untargeted and targeted lipidomics. Compared with rapid elongation fibers, the lipid intensity of 16 sub-classes and 56 molecular species decreased, while only 7 sub-classes and 26 molecular species increased in the fibers at the stage of secondary cell wall deposition. Unexpectedly, at the rapid elongation stage, 20 sub-classes and 60 molecular species increased significantly, while only 5 sub-classes and 8 molecular species decreased in the ligon lintless-1 (li-1) mutant compared with its wild-type Texas Maker-1 (TM-1). Particularly, campesteryl, sitosteryl, and total steryl ester increased by 21.8-, 48.7-, and 45.5-fold in the li-1 fibers, respectively. All the molecular species of sphingosine-1-P, phytoceramide-OHFA, and glucosylceramide increased while all sphingosine, phytosphingosine, and glycosyl inositol phospho ceramides decreased in the li-1 fibers. Similarly, the different expression genes between the mutant and wild type were enriched in many pathways involved in the lipid metabolism. Furthermore, the number of lipid droplets also increased in the li-1 leaf and fiber cells when compared with the wild type. These results illuminated that fiber cell elongation being blocked in the li-1 mutant was not due to a lack of lipids, but rather lipid over-accumulation (obesity), which may result from the disruption of sphingolipid and sterol metabolism. This study provides a new perspective for further studying the regulatory mechanisms of fiber development.
Mitochondrial COX3 and tRNA Gene Variants Associated with Risk and Prognosis of Idiopathic Pulmonary Fibrosis
Authors: Lee Li-Na , Jan I-Shiow , Chou Wen-Ru , Liu Wei-Lun , Kuo Yen-Liang , Chang Chih-Yueh , Chang Hsiu-Ching , Liu Jia-Luen , Hsu Chia-Lin , Lin Chia-Nan , Chao Ke-Yun , Tseng Chi-Wei , Lee I-Hsien , Wang Jann-Tay , Wang Jann-Yuan
Published: February 2025

Idiopathic pulmonary fibrosis (IPF) has been associated with mitochondrial dysfunction. We investigated whether mitochondrial DNA variants in peripheral blood leukocytes (PBLs), which affect proteins of the respiratory chain and mitochondrial function, could be associated with an increased risk and poor prognosis of IPF. From 2020 to 2022, we recruited 36 patients (age: 75.3 ± 8.5; female: 19%) with IPF, and 80 control subjects (age: 72.3 ± 9.0; female: 27%). The mitochondrial genome of peripheral blood leukocytes was determined using next-generation sequencing. During a 45-month follow-up, 10 (28%) patients with IPF remained stable and the other 26 (72%) progressed, with 12 (33%) mortalities. IPF patients had more non-synonymous (NS) variants (substitution/deletion/insertion) in mitochondrial COX3 gene (coding for subunit 3 of complex IV of the respiratory chain), and more mitochondrial tRNA variants located in the anticodon (AC) stem, AC loop, variable loop, T-arm, and T-loop of the tRNA clover-leaf structure in PBLs than the control group. The succumbed IPF patients were older, had lower initial diffusion capacity, and higher initial fibrosis score on high-resolution computerized tomography (HRCT) than the alive group. NS variants in mitochondrial COX3 gene and tRNA variants in PBLs were associated with shorter survival. Our study shows that (1) leukocyte mitochondrial COX3 NS variants are associated with risk and prognosis of IPF; (2) leukocyte mitochondrial tRNA variants located in the AC stem, AC loop, variable loop, T-arm, and T-loop of the tRNA clover-leaf structure are associated with risk, and the presence of tRNA variants is associated with poor prognosis of IPF.
Differential Regulation of miRNA and Protein Profiles in Human Plasma-Derived Extracellular Vesicles via Continuous Aerobic and High-Intensity Interval Training
Authors: Wang Zhenghao , Ou Yiran , Zhu Xinyue , Zhou Ye , Zheng Xiaowei , Zhang Meixia , Li Sheyu , Yang Shao-Nian , Juntti-Berggren Lisa , Berggren Per-Olof , Zheng Xiaofeng
Published: February 2025

Both continuous aerobic training (CAT) and high-intensity interval training (HIIT) are recommended to promote health and prevent diseases. Exercise-induced circulating extracellular vesicles (EX-EVs) have been suggested to play essential roles in mediating organ crosstalk, but corresponding molecular mechanisms remain unclear. To assess and compare the systemic effects of CAT and HIIT, five healthy male volunteers were assigned to HIIT and CAT, with a 7-day interval between sessions. Plasma EVs were collected at rest or immediately after each training section, prior to proteomics and miRNA profile analysis. We found that the differentially expressed (DE) miRNAs in EX-EVs were largely involved in the regulation of transcriptional factors, while most of the DE proteins in EX-EVs were identified as non-secreted proteins. Both CAT and HIIT play common roles in neuronal signal transduction, autophagy, and cell fate regulation. Specifically, CAT showed distinct roles in cognitive function and substrate metabolism, while HIIT was more associated with organ growth, cardiac muscle function, and insulin signaling pathways. Interestingly, the miR-379 cluster within EX-EVs was specifically regulated by HIIT, involving several biological functions, including neuroactive ligand–receptor interaction. Furthermore, EX-EVs likely originate from various tissues, including metabolic tissues, the immune system, and the nervous system. Our study provides molecular insights into the effects of CAT and HIIT, shedding light on the roles of EX-EVs in mediating organ crosstalk and health promotion.
Genetic Diversity and Environmental Adaptation Signatures of the Great Seahorse (Hippocampus kelloggi) in the Coastal Regions of the Indo-Pacific as Revealed by Whole-Genome Re-Sequencing
Authors: Hao Wen-Xin , Zhang Ying-Yi , Wang Xin , Qu Meng , Wan Shi-Ming , Lin Qiang
Published: February 2025

The great seahorse (Hippocampus kelloggi) is one of the larger species within the seahorse group and is widely distributed in coastal areas of the Indo-Pacific. However, the natural resources of this species continue to decrease, rendering it a vulnerable species that faces a high risk of extinction. Therefore, there is an urgent need to conduct research on the genetic diversity of this species to protect its genetic resources. In this study, we conducted whole-genome re-sequencing (WGRS) on three H. kelloggi populations from the Red Sea (RS, n = 30), the Andaman Sea (AS, n = 13), and the South China Sea (SCS, n = 13), and a total of 1,398,936 high-quality single-nucleotide polymorphisms (SNPs) were identified. The results indicate that the average observed heterozygosity (Ho) and the average expected heterozygosity (He) for the RS, AS, and SCS populations are 0.2031 and 0.1987, 0.1914 and 0.1822, and 0.2083 and 0.2001, respectively. The three geographic populations exhibit a high degree of genetic differentiation with only a minimal gene flow between them. Consistently, in a population structure analysis, the three groups are also clearly distinguished, which is consistent with the results of the population differentiation coefficient. Demographic analyses revealed that the effective population size (Ne) of the SCS population underwent a dramatic bottleneck during the Last Glacial Maximum (LGM), followed by a substantial recovery, whereas the RS and AS populations maintained stable Ne values throughout this period. To investigate adaptive responses to climate change in the SCS population, we employed selective elimination analysis, which identified 21 candidate genes potentially involved in environmental adaptation. Of particular significance were myo5a, hps4, znf385a, msh3, and pfkfb4, which likely play crucial roles in the adaptive mechanisms of H. kelloggi. This comprehensive study not only illuminates the genetic diversity patterns of H. kelloggi but also provides a valuable foundation for future investigations into the species’ evolutionary adaptations.
NSCLC: Current Evidence on Its Pathogenesis, Integrated Treatment, and Future Perspectives
Authors: Tahayneh Kareem , Idkedek Mayar , Abu Akar Firas
Published: February 2025

Non-small cell lung carcinoma (NSCLC) comprises the majority of lung cancer cases, characterized by a complex interplay of genetic alterations, environmental factors, and molecular pathways contributing to its pathogenesis. This article highlights the multifaceted pathogenesis of NSCLC and discusses screening and integrated strategies for current treatment options. NSCLC is an evolving field with various aspects including the underlying molecular alterations, oncogenic driver mutations, and immune microenvironment interactions that influence tumor progression and response to therapy. Surgical treatment remains the most applicable curative option, especially in the early stages of the disease, adjuvant chemotherapy may add benefits to previously resected patients. Combined Radio-chemotherapy can also be used for palliative purposes. There are various future perspectives and advancing horizons in NSCLC management, encompassing novel therapeutic modalities and their applications, such as CAR-T cell therapy, antibody-drug conjugates, and gene therapies. On the other hand, it’s crucial to highlight the efficacy of innovative modalities of Immunotherapy and immune checkpoint inhibitors that are nowadays widely used in treatment of NSCLC. Moreover, the latest advancements in molecular profiling techniques and the development of targeted therapies designed for specific molecular alterations in NSCLC play a significant role in its treatment. In conclusion, personalized approaches are a cornerstone of successful treatment, and they are based on a patient’s unique molecular profile, tumor characteristics, and host factors. Entitling the concept of individualized treatment strategies requires proper patient selection, taking into consideration mechanisms of resistance, and investigating potential combination therapies, to achieve the optimal impact on long-term survival.
Innovative and Sustainable Advances in Polymer Composites for Additive Manufacturing: Processing, Microstructure, and Mechanical Properties
Authors: Saharudin Mohd Shahneel , Ullah Asif , Younas Muhammad
Published: February 2025

Additive manufacturing (AM) has revolutionised the production of customised components across industries such as the aerospace, automotive, healthcare, electronics, and renewable energy industries. Offering unmatched design freedom, reduced time-to-market, and minimised material waste, AM enables the fabrication of high-quality, customised products with greater sustainability compared to traditional methods like machining and injection moulding. Additionally, AM reduces energy consumption, resource requirements, and CO2 emissions throughout a material’s lifecycle, aligning with global sustainability goals. This paper highlights insights into the sustainability of AM polymers, comparing bio-based and traditional polymers. Bio-based polymers exhibit lower carbon footprints during production but may face challenges in durability and mechanical performance. Conversely, traditional polymers, while more robust, require higher energy inputs and contribute to greater carbon emissions. Polymer composites tailored for AM further enhance material properties and support the development of innovative, eco-friendly solutions. This Special Issue brings together cutting-edge research on polymer composites in AM, focusing on processing techniques, microstructure–property relationships, mechanical performance, and sustainable manufacturing practices. These advancements underscore AM’s transformative potential to deliver versatile, high-performance solutions across diverse industries.
Preliminary Investigation on Biodegradable Ureteral Stents Using 3D Printing
Authors: Chetan Chirag , James Sagil
Published: February 2025

The prevalence of kidney stones, a significant urological health concern, necessitates advancements in the management and treatment methods, particularly in the domain of ureteral stents. This study explores the feasibility and potential benefits of utilizing three biodegradable polymers—Polylactic Acid (PLA), Tough Polylactic Acid (Tough PLA), and Polylactic Acid/Poly-hydroxybutyrate (PLA/PHB)—for the fabrication of 3D-printed ureteral stents tailored to patient-specific needs. Through the integration of CAD and Fused Deposition Modeling (FDM) 3D printing technology, ureteral stents were successfully produced, demonstrating key advantages in terms of biodegradability and mechanical properties. The study involved a rigorous evaluation of the biodegradability, tensile strength, and hardness of the stents. Biodegradability tests performed in a simulated physiological environment revealed that PLA/PHB and Tough PLA stents exhibited higher degradation rates compared to PLA, aligning with the requirements for temporary urinary tract support. Tensile strength testing indicated that while PLA showed the highest strength, PLA/PHB and Tough PLA stents provided beneficial ductility, reducing the risk of blockage due to material breakage. Hardness assessments classified PLA/PHB stents as medium soft, optimizing patient comfort during the stenting period. These findings demonstrate the potential of using biodegradable polymers to produce ureteral stents that could eliminate the need for removal procedures, thereby enhancing patient recovery and comfort.