bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Osteoconduction of Biphasic Calcium Phosphate Ceramic in the Calvaria of Rats: Microscopic and Histometric Analysis
Authors: Puttini Igor de Oliveira , Poli Pier Paolo , Maiorana Carlo , Vasconcelos Igor Rodrigues de , Schmidt Luis Eduardo , Colombo Luara Teixeira , Hadad Henrique , Santos Gabriel Mulinari dos , Carvalho Paulo Sergio Perri de , Souza Francisley Ávila
Published: January 2019

(1) Background: Evaluate the osteoconduction capability of a biphasic calcium phosphate (BCP) ceramic composed of hydroxyapatite and β-tricalcium phosphate 60%/40% in a rat model. (2) Methods: In the calvarial bone of 54 adult male rats, 7-mm diameter critical size defects were performed. The animals were randomly allocated to three experimental groups according to the type of material: blood clot (BCG), blood clot covered with a bovine-derived collagen membrane (MBCG), and BCP ceramic covered with a bovine-derived collagen membrane (BCPG). In each group, 6 animals were euthanatized at post-operative days 7, 30, and 60 for histological and histometric analysis. (3) Results: The qualitative analysis revealed the persistence of the collagen membrane at seven days, with no relevant newly bone formation in all groups. At 30 days, centripetal bone formation was observed residual particles of the biomaterial surrounded by fibroblasts noted in the BCPG. At 60 days, while BCG and MBCG showed a partial maturation with the central part of the defect populated by a fibrous connective tissue, in the BCPG the critical area was entirely occupied by newly formed bone. In the intra groups analysis was noted a significant increase in new bone formation during the experimental period (p < 0.05). At 60 days, BCPG showed a higher percentage area of new bone formation (p < 0.05). (4) Conclusion: BCP promoted a new bone formation by osteoconduction and might be considered a valid alternative in bone regeneration procedures.
The “Journal of Functional Morphology and Kinesiology” Journal Club Series: Highlights on Recent Papers in Exercise and Osteoarthritis
Authors: Musumeci Giuseppe , Szychlinska Marta A. , Herzog Walter
Published: January 2019

We are glad to introduce the eleventh Journal Club. This edition is focused on several relevant studies published in the last years in the field of exercise and osteoarthritis, chosen by our Editorial Board members and their colleagues. We hope to stimulate your curiosity in this field and to share with you the passion for sport seen also from the scientific point of view. The Editorial Board members wish you an inspiring lecture.
Efficient FPGA Implementation of Automatic Nuclei Detection in Histopathology Images
Authors: Zhou Haonan , Machupalli Raju , Mandal Mrinal
Published: January 2019

Accurate and efficient detection of cell nuclei is an important step towards the development of a pathology-based Computer Aided Diagnosis. Generally, high-resolution histopathology images are very large, in the order of billion pixels, therefore nuclei detection is a highly compute intensive task, and software implementation requires a significant amount of processing time. To assist the doctors in real time, special hardware accelerators, which can reduce the processing time, are required. In this paper, we propose a Field Programmable Gate Array (FPGA) implementation of automated nuclei detection algorithm using generalized Laplacian of Gaussian filters. The experimental results show that the implemented architecture has the potential to provide a significant improvement in processing time without losing detection accuracy.
Experimental Validation of Smoothed Particle Hydrodynamics on Generation and Propagation of Water Waves
Authors: Trimulyono Andi , Hashimoto Hirotada
Published: January 2019

This paper is aimed to validate smoothed particle hydrodynamics (SPH) on the generation and propagation of water waves. It is a classical problem in marine engineering but a still important problem because there is a strong demand to generate intended nonlinear water waves and to predict complicated interactions between nonlinear water waves and fixed/floating bodies, which is indispensable for further ocean utilization and development. A dedicated experiment was conducted in a large wave basin of Kobe University equipped with a piston-type wavemaker, at three water depths using several amplitudes and periods of piston motion for the validation of SPH mainly on the long-distance propagation of water waves. An SPH-based two-dimensional numerical wave tank (NWT) is used for numerical simulation and is accelerated by a graphics processing units (GPU), assuming future applications to realistic engineering problems. In addition, comparison of large-deformation of shallow water waves, when passing over a fixed box-shape obstacle, is also investigated to discuss the applicability to wave-structure interaction problems. Finally, an SPH-based three-dimensional NWT is also validated by comparing with an experiment and two-dimensional simulation. Through these validation studies, detailed discussion on the accuracy of SPH simulation of water waves is made as well as providing a recommended set of SPH parameters.
Pharmacogenomics of Novel Direct Oral Anticoagulants: Newly Identified Genes and Genetic Variants
Authors: Kanuri Sri H. , Kreutz Rolf P.
Published: January 2019

Direct oral anticoagulants (DOAC) have shown an upward prescribing trend in recent years due to favorable pharmacokinetics and pharmacodynamics without requirement for routine coagulation monitoring. However, recent studies have documented inter-individual variability in plasma drug levels of DOACs. Pharmacogenomics of DOACs is a relatively new area of research. There is a need to understand the role of pharmacogenomics in the interpatient variability of the four most commonly prescribed DOACs, namely dabigatran, rivaroxaban, apixaban, and edoxaban. We performed an extensive search of recently published research articles including clinical trials and in-vitro studies in PubMed, particularly those focusing on genetic loci, single nucleotide polymorphisms (SNPs), and DNA polymorphisms, and their effect on inter-individual variation of DOACs. Additionally, we also focused on commonly associated drug-drug interactions of DOACs. CES1 and ABCB1 SNPs are the most common documented genetic variants that contribute to alteration in peak and trough levels of dabigatran with demonstrated clinical impact. ABCB1 SNPs are implicated in alteration of plasma drug levels of rivaroxaban and apixaban. Studies conducted with factor Xa, ABCB1, SLCOB1, CYP2C9, and VKORC1 genetic variants did not reveal any significant association with plasma drug levels of edoxaban. Pharmacokinetic drug-drug interactions of dabigatran are mainly mediated by p-glycoprotein. Strong inhibitors and inducers of CYP3A4 and p-glycoprotein should be avoided in patients treated with rivaroxaban, apixaban, and edoxaban. We conclude that some of the inter-individual variability of DOACs can be attributed to alteration of genetic variants of gene loci and drug-drug interactions. Future research should be focused on exploring new genetic variants, their effect, and molecular mechanisms that contribute to alteration of plasma levels of DOACs.
Fusing Thermopile Infrared Sensor Data for Single Component Activity Recognition within a Smart Environment
Authors: Burns Matthew , Morrow Philip , Nugent Chris , McClean Sally
Published: January 2019

To provide accurate activity recognition within a smart environment, visible spectrum cameras can be used as data capture devices in solution applications. Privacy, however, is a significant concern with regards to monitoring in a smart environment, particularly with visible spectrum cameras. Their use, therefore, may not be ideal. The need for accurate activity recognition is still required and so an unobtrusive approach is addressed in this research highlighting the use of a thermopile infrared sensor as the sole means of data collection. Image frames of the monitored scene are acquired from a thermopile infrared sensor that only highlights sources of heat, for example, a person. The recorded frames feature no discernable characteristics of people; hence privacy concerns can successfully be alleviated. To demonstrate how thermopile infrared sensors can be used for this task, an experiment was conducted to capture almost 600 thermal frames of a person performing four single component activities. The person’s position within a room, along with the action being performed, is used to appropriately predict the activity. The results demonstrated that high accuracy levels, 91.47%, for activity recognition can be obtained using only thermopile infrared sensors.
Role of Mineral Surfaces in Prebiotic Chemical Evolution. In Silico Quantum Mechanical Studies
Authors: Rimola Albert , Sodupe Mariona , Ugliengo Piero
Published: January 2019

There is a consensus that the interaction of organic molecules with the surfaces of naturally-occurring minerals might have played a crucial role in chemical evolution and complexification in a prebiotic era. The hurdle of an overly diluted primordial soup occurring in the free ocean may have been overcome by the adsorption and concentration of relevant molecules on the surface of abundant minerals at the sea shore. Specific organic–mineral interactions could, at the same time, organize adsorbed molecules in well-defined orientations and activate them toward chemical reactions, bringing to an increase in chemical complexity. As experimental approaches cannot easily provide details at atomic resolution, the role of in silico computer simulations may fill that gap by providing structures and reactive energy profiles at the organic–mineral interface regions. Accordingly, numerous computational studies devoted to prebiotic chemical evolution induced by organic–mineral interactions have been proposed. The present article aims at reviewing recent in silico works, mainly focusing on prebiotic processes occurring on the mineral surfaces of clays, iron sulfides, titanium dioxide, and silica and silicates simulated through quantum mechanical methods based on the density functional theory (DFT). The DFT is the most accurate way in which chemists may address the behavior of the molecular world through large models mimicking chemical complexity. A perspective on possible future scenarios of research using in silico techniques is finally proposed.
A Facile Approach for Preparing Ag Functionalized Nonwoven Polypropylene Membrane to Improve Its Electrical Conductivity and Electromagnetic Shielding Performance
Authors: Shao Dongfeng , Zhang Hongwei , Tao Lizhen , Cao Kan , Wei Qufu
Published: January 2019

The commonly used preparation methods of polypropylene functionalization require special equipment to be put into use or take a long time, which limits its application. Therefore, a simple and economical method for preparing silver functionalized nonwoven polypropylene membrane was studied herein. Triethanolamine was first coated on the surface of the polypropylene, and then Ag was deposited on the surface of polypropylene using a continuous reduction reaction of triethanolamine and silver ions. Surface morphology, crystal structure, and surface chemistry during the preparation of Ag functionalized nonwoven polypropylene were investigated. The electrical conductivity, electromagnetic shielding properties, and washing durability of the treated nonwoven polypropylene were also studied. It was found that Ag was uniformly deposited on the surface of the nonwoven polypropylene, and the coating reaction did not change the chemical structure of the polypropylene. The crystallinity and thermal stability of polypropylene were improved after silver coated polypropylene. The washing experiment results showed that the weight gain rate of the treated nonwoven relative to the untreated sample after the 90 min washing ranged from 6.72% to 9.64%. The resistance test results showed that the maximum surface resistivity of Ag coated nonwoven polypropylene was about 1.95 × 105 Ω, which was 64,615 times lower than the original. In addition, the results showed that the maximum electromagnetic shielding effectiveness of the Ag coated nonwoven polypropylene was about 71.6 dB, showing a very good electromagnetic shielding effect.
Application of Optimization to Select Contractors to Develop Strategies and Policies for the Development of Transport Infrastructure
Authors: Wang Chia-Nan , Le Tien-Muoi , Nguyen Han-Khanh
Published: January 2019

Many factors influence the efficiency and quality of transport works. In particular, consultants and construction contractors of these works play important roles, and critical factors directly affect the quality of traffic works. If the quality of consultancy and construction is good, the project will reduce the total investment; if the contractor is good, the completion time of the new project is guaranteed, thus reducing construction costs. The longer the construction time is, the higher the cost of the project. In this study, the authors used optimal algorithms to evaluate past, present, and future contractors’ technical, technological, and performance effectiveness. Research results show that bidders are divided into three groups: highly effective bidders, stable contractors, and inefficient groups. Research results for this subject will help the government, regulatory agencies, and investors select good contractors as the basis for developing strategies and policies for the development of transport infrastructure.
Evolution of an Exponential Polynomial Family of Discrete Dynamical Systems
Authors: Solis Francisco
Published: January 2019

In this paper, we introduce and analyze a family of exponential polynomial discrete dynamical systems that can be considered as functional perturbations of a linear dynamical system. The stability analysis of equilibria of this family is performed by considering three different parametric scenarios, from which we show the intricate and complex dynamical behavior of their orbits.
Effects of Qigong Exercise on Non-Motor Symptoms and Inflammatory Status in Parkinson’s Disease: A Protocol for a Randomized Controlled Trial
Authors: Moon Sanghee , Sarmento Caio V. M. , Smirnova Irina V. , Colgrove Yvonne , Lyons Kelly E. , Lai Sue M. , Liu Wen
Published: January 2019

Background: Non-motor symptoms such as sleep disturbance, cognitive decline, fatigue, anxiety, and depression in Parkinson’s disease (PD) impact quality of life. Increased levels of pro-inflammatory cytokines in individuals with PD have been reported, which may contribute to non-motor symptoms. A mind-body exercise, Qigong, has demonstrated benefits across different medical conditions. However, a lack of evidence causes clinicians and patients to be uncertain about the effects of Qigong in individuals with PD. This study will examine the effects of Qigong on non-motor symptoms and inflammatory status in individuals with PD. Methods: Sixty individuals with PD will be recruited. Qigong and sham Qigong group (n = 30 for each) will receive a 12-week intervention. Participants will practice their assigned exercise at home (2×/day) and attend routinely group exercise meetings. Results: Clinical questionnaires and neuropsychological tests will measure non-motor symptoms including sleep quality (primary outcome). Biomarker assays will measure inflammatory status. A two-way mixed-design analysis of variance (ANOVA) will be utilized. Conclusions: This study may generate evidence for the benefits of Qigong on non-motor symptoms of PD and the effect on inflammatory status. Findings may lead to the development of a novel, safe, and cost-effective rehabilitation approach for individuals with PD.
Investigation on the High-Temperature Oxidation Resistance of Ni-(3~10) Ta and Ni-(3~10) Y Alloys
Authors: Duan Hang , Liu Yan , Lin Tiesong , Zhang Hui , Huang Zhengren
Published: January 2019

Ni-(3~10) Ta and Ni-(3~10) Y alloys were fabricated by vacuum arc melting. The oxidation resistance of the alloys was studied by cyclic and isothermal oxidation tests at 800 °C in static air. The present work focused on the investigation of the effects of the alloying elements (Ta and Y) on the oxidation behavior of Ni-based alloys. The oxidation behavior of alloys was evaluated by mass gain, composition, as well as the microstructure of oxidized products. The experimental results indicated that Ta at a low content (3 wt %) had a positive role in enhancing oxidation resistance by decreasing the oxygen vacancy concentration of the oxide layer to prevent the inward diffusion of oxygen during oxidation, and the mass gain decreased from 2.9 mg·cm−2 to 1.7 mg·cm−2 (800 °C/200 h), while Y (3~10 wt %) degraded the oxidation resistance. However, it is worth mentioning that the pinning effect of Y2O3 increased the adhesion between the substrate and oxide layer by changing the growing patterns of the oxide layer from a plane growth to fibrous growth. Among the results, the bonding of the substrate and oxide layer was best in the Ni-3Y alloys.
Laser Hybrid Butt Welding of Large Thickness Naval Steel
Authors: Churiaque Cristina , Chludzinski Mariane , Porrua-Lara Manuel , Dominguez-Abecia Antonio , Abad-Fraga Francisco , Sánchez-Amaya Jose Maria
Published: January 2019

Plates joining is one of the first stage at large vessels manufacturing line, process conditioning the whole shipbuilding production. Laser Arc Hybrid Welding (LAHW) process is nowadays providing promising results for large thickness naval steel, being primarily used for welding plates with thicknesses between 6 to 15 mm, reaching up to 51 mm. In addition to this high penetration ability, LAHW allows increasing the production rates. Therefore, this technology is proposed as an alternative to conventional welding processes in shipbuilding, as it integrates the advantages of laser and arc welding, providing high process stability, high welding speed and penetration, narrow weld beads with a low heat input and good metallurgical properties. The present review reports the most representative investigation regarding the use of this technology to join large thickness flat panels of naval steel. It includes a summary of the most influential process variables, equipment characteristics, material properties, naval regulations, as well as microstructural characterisation and mechanical properties of joints. This review is thought to help readers from different backgrounds, covering from non-expert on welding or on naval sector, to industrial LAHW applicators and researchers. The industrial need of performing one single pass procedure to assure high quality welds of high thickness is suggested as one of the key aspects for future investigations.
Experimental Determinations of Mixing Times in the IronArc Pilot Plant Process
Authors: Bölke Kristofer , Ersson Mikael , Andersson Nils Å. I. , Imris Matej , Jönsson Pär G.
Published: January 2019

IronArc is a newly developed technology and an emerging future process for pig iron production. The long-term goal with this technology is to reduce the CO2 emissions and energy consumption compared to existing technologies. The production rate of this process is dependent on the stirring, which was investigated in the pilot plant process by measuring the mixing time in the slag bath. Moreover, slag investigations were done both based on light optical microscope studies as well as by Thermo-Calc calculations in order to determine the phases of the slag during operation. This was done because the viscosity (which is another important parameter) is dependent on the liquid and solid fractions of the slag. The overall results show that it was possible to determine the mixing time by means of the addition of a tracer (MnO2 powder) to the slag. The mixing time for the trials showed that the slag was homogenized after seconds. For two of the trials, homogenization had already been reached in the second sample after tracer addition, which means ≤8 s. The phase analysis from the slag indicated that the slag is in a liquid state during the operation of the process.
Dissimilar Metals Laser Welding between DP1000 Steel and Aluminum Alloy 1050
Authors: Pereira António B. , Cabrinha Ana , Rocha Fábio , Marques Pedro , Fernandes Fábio A. O. , Alves de Sousa Ricardo J.
Published: January 2019

The welding of dissimilar metals was carried out using a pulsed Nd: YAG laser to join DP1000 steel and an aluminum alloy 1050 H111. Two sheets of each metal, with 30 × 14 × 1 mm3, were lap welded, since butt welding proved to be nearly impossible due to the huge thermal conductivity differences and melting temperature differences of these materials. The aim of this research was to find the optimal laser welding parameters based on the mechanical and microstructure investigations. Thus, the welded samples were then subjected to tensile testing to evaluate the quality of the joining operation. The best set of welding parameters was replicated, and the welding joint obtained using these proper parameters was carefully analyzed using optical and scanning electron microscopes. Despite the predicted difficulties of welding two distinct metals, good quality welded joints were achieved. Additionally, some samples performed satisfactorily well in the mechanical tests, reaching tensile strengths close to the original 1050 aluminum alloy.
Empirical Modeling of Zn/ZnO Nanoparticles Decorated/Conjugated with Fotolon (Chlorine e6) Based Photodynamic Therapy towards Liver Cancer Treatment
Authors: Iqbal Seemab , Fakhar-e-Alam Muhammad , Atif M. , Ahmed Nasar , -ul-Ahmad Aqrab , Amin N. , Alghamdi Raed ahmed , Hanif Atif , Farooq W. Aslam
Published: January 2019

The current study is based on Zn/ZnO nanoparticles photodynamic therapy (PDT) mediated effects on healthy liver cells and cancerous cells. The synthesis of Zn/ZnO nanoparticles was accomplished using chemical and hydrothermal methods. The characterization of the synthesized nanoparticles was carried out using manifold techniques (e.g., transmission electron microscopy (TEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDS)). In order to study the biotoxicity of the grown nanoparticles, they were applied individually and in conjunction with the third generation photosensitiser Fotolon (Chlorine e6) in the in vivo model of the normal liver of the Wister rat, and in the in vitro cancerous liver (HepG2) model both in the dark and under a variety of laser exposures (630 nm, Ultraviolet (UV) light). The localization of ZnO nanoparticles was observed by applying fluorescence spectroscopy on a 1 cm2 selected area of normal liver, whereas the in vitro cytotoxicity and reactive oxygen species (ROS) detection were carried out by calculating the loss in the cell viability of the hepatocellular model by applying a neutral red assay (NRA). Furthermore, a statistical analysis is carried out and it is ensured that the p value is less than 0.05. Thus, the current study has highlighted the potential for applying Zn/ZnO nanoparticles in photodynamic therapy that would lead to wider medical applications to improve the efficiency of cancer treatment and its biological aspect study.
Dextran as a Resorbable Coating Material for Flexible Neural Probes
Authors: Kil Dries , Bovet Carmona Marta , Ceyssens Frederik , Deprez Marjolijn , Brancato Luigi , Nuttin Bart , Balschun Detlef , Puers Robert
Published: January 2019

In the quest for chronically reliable and bio-tolerable brain interfaces there has been a steady evolution towards the use of highly flexible, polymer-based electrode arrays. The reduced mechanical mismatch between implant and brain tissue has shown to reduce the evoked immune response, which in turn has a positive effect on signal stability and noise. Unfortunately, the low stiffness of the implants also has practical repercussions, making surgical insertion extremely difficult. In this work we explore the use of dextran as a coating material that temporarily stiffens the implant, preventing buckling during insertion. The mechanical properties of dextran coated neural probes are characterized, as well as the different parameters which influence the dissolution rate. Tuning parameters, such as coating thickness and molecular weight of the used dextran, allows customization of the stiffness and dissolution time to precisely match the user’s needs. Finally, the immunological response to the coated electrodes was analyzed by performing a histological examination after four months of in vivo testing. The results indicated that a very limited amount of glial scar tissue was formed. Neurons have also infiltrated the area that was initially occupied by the dissolving dextran coating. There was no noticeable drop in neuron density around the site of implantation, confirming the suitability of the coating as a temporary aid during implantation of highly flexible polymer-based neural probes.
Growth Process and CQDs-modified Bi4Ti3O12 Square Plates with Enhanced Photocatalytic Performance
Authors: Zhao Xinxin , Yang Hua , Cui Ziming , Wang Xiangxian , Yi Zao
Published: January 2019

Bi4Ti3O12 square plates were synthesized via a hydrothermal route, and their growth process was systematically investigated. Carbon quantum dots (CQDs) were prepared using glucose as the carbon source, which were then assembled on the surface of Bi4Ti3O12 square plates via a hydrothermal route with the aim of enhancing the photocatalytic performance. XRD (X-ray powder diffraction), SEM (scanning electron microscopy), TEM (transmission electron microscopy), UV-vis DRS (diffuse reflectance spectroscopy), XPS (X-ray photoelectron spectroscopy), FTIR (Fourier transform infrared spectroscopy), PL (photoluminescence) spectroscopy, EIS (electrochemical impedance spectroscopy) and photocurrent spectroscopy were used to systematically characterize the as-prepared samples. It is demonstrated that the decoration of CQDs on Bi4Ti3O12 plates leads to an increased visible light absorption, slightly increased bandgap, increased photocurrent density, decreased charge-transfer resistance, and decreased PL intensity. Simulated sunlight and visible light were separately used as a light source to evaluate the photocatalytic activity of the samples toward the degradation of RhB in aqueous solution. Under both simulated sunlight and visible light irradiation, CQDs@Bi4Ti3O12 composites with an appropriate amount of CQDs exhibit obviously enhanced photocatalytic performance. However, the decoration of excessive CQDs gives rise to a decrease in the photocatalytic activity. The enhanced photocatalytic activity of CQDs-modified Bi4Ti3O12 can be attributed to the following reasons: (1) The electron transfer between Bi4Ti3O12 and CQDs promotes an efficient separation of photogenerated electron/hole pairs in Bi4Ti3O12; (2) the up-conversion photoluminescence emitted from CQDs could induce the generation of additional electron/hole pairs in Bi4Ti3O12; and (3) the photoexcited electrons in CQDs could participate in the photocatalytic reactions.
Scanning MEMS Mirror for High Definition and High Frame Rate Lissajous Patterns
Authors: Seo Yeong-Hyeon , Hwang Kyungmin , Kim Hyunwoo , Jeong Ki-Hun
Published: January 2019

Scanning MEMS (micro-electro-mechanical system) mirrors are attractive given their potential use in a diverse array of laser scanning display and imaging applications. Here we report on an electrostatic MEMS mirror for high definition and high frame rate (HDHF) Lissajous scanning. The MEMS mirror comprised a low Q-factor inner mirror and frame mirror, which provided two-dimensional scanning at two similar resonant scanning frequencies with high mechanical stability. The low Q inner mirror enabled a broad frequency selection range. The high definition and high frame rate (HDHF) Lissajous scanning of the MEMS mirror was achieved by selecting a set of scanning frequencies near its resonance with a high greatest common divisor (GCD) and a high total lobe number. The MEMS mirror had resonant scanning frequencies at 5402 Hz and 6702 Hz in x and y directions, respectively. The selected pseudo-resonant frequencies of 5450 Hz and 6700 Hz for HDHF scanning provided 50 frames per second with 94% fill factor in 256 × 256 pixels. This Lissajous MEMS mirror could be utilized for assorted HDHF laser scanning imaging and display applications.
Properties and Application of Backfill Materials in Coal Mines in China
Authors: Zhang Jixiong , Li Meng , Taheri Abbas , Zhang Weiqing , Wu Zhongya , Song Weijian
Published: January 2019

Coal is the basic resource underpinning energy generation in China, however, constant, large-scale mining of coal results in many problems such as ecological destruction of mining areas. As a result, backfilling of solid waste underground is proposed to control strata and surface subsidence and to protect the environment. At present, these materials, such as granular material, cemented material and high-water-content materials are mainly used for backfilling. This study summarised the types of backfill materials that are used in coal mines in China along with the backfilling process. Moreover, distribution and characteristics of mines backfilled with these backfill materials were obtained and analysed. Considering the socio-environmental aspects that affect backfilling, this research proposed a guideline for the selection of backfill materials and then analysed specific engineering cases of three backfill materials. In addition, the future development of backfill materials was discussed. With extensive extraction of shallow coal resources in China and, therefore, rapid depletion of coal resources in eastern regions of China, coal mining depth is increasing significantly. As a result, it is required to investigate new backfill materials suited for the deep high-stress environment.
Structural Control of Ore Deposits: The Role of Pre-Existing Structures on the Formation of Mineralised Vein Systems
Authors: CHAUVET Alain
Published: January 2019

The major role played by pre-existing structures in the formation of vein-style mineral deposits is demonstrated with several examples. The control of a pre-existing decollement level on the formation of a crustal extension-related (collapse) gold deposit is first illustrated in the Quadrilátero Ferrífero from Brazil. Shear zone and decollement structures were also examined and shown to control veins formation by three distinct processes: (i) re-aperture and re-using of wrench shear zones in the case of Shila gold mines (south Peru); (ii) remobilisation of metal in volcanic-hosted massive sulphide (VHMS) deposit by subsequent tectonic events and formation of a secondary stockwork controlled by structures created during this event (Iberian Pyrite Belt, Spain); (iii) formation of economic stockwork by contrasting deformation behaviours between ductile black schist versus brittle more competent dolomite (Cu-Ifri deposit, Morocco). Two examples involve changing of rheological competence within zones affected by deformation and/or alteration in order to receive the mineralisation (case studies of Achmmach, Morocco, and Mina Soriana, Spain). The last case underscores the significance of the magmatic–hydrothermal transition in the formation of mesothermal gold deposits (Bruès mine, Spain). All these examples clearly demonstrate the crucial role played by previously formed structures and/or texture in the development and formation of ore deposits.
Long-Lived Mantle Plume and Polyphase Evolution of Palaeoproterozoic PGE Intrusions in the Fennoscandian Shield
Authors: Bayanova Tamara , Korchagin Aleksey , Mitrofanov Alexander , Serov Pavel , Ekimova Nadezhda , Nitkina Elena , Kamensky Igor , Elizarov Dmitry , Huber Milosh
Published: January 2019

The NE Fennoscandian Shield comprises the Northern Belt in Finland and the Southern Belt in Karelia. They host mafic-ultramafic layered Cu-Ni-Cr and Pt-Pd-bearing intrusions. Precise U-Pb and Sm-Nd analyses indicate the 130-Ma evolution of these intrusions, with major events at 2.53, 2.50, 2.45, and 2.40 Ga. Barren phases were dated at 2.53 Ga for orthopyroxenites and olivine gabbro in the Fedorovo-Pansky massif. PGE-bearing phases of gabbronorites (Pechenga, Fedorovo-Pansky, Monchetundra massifs) and norites (Monchepluton) are 2.50 Ga old. Anorthosites of Mt. Generalskaya (Pechenga), the Fedorovo-Pansky, and Monchetundra massifs occurred at 2.45 Ga. This event produced layered PGE-bearing intrusions in Finland (Penikat, Kemi, Koitelainen) and mafic intrusions in Karelia. The Imandra lopolith dikes occurred at the final phase (2.40 Ga). Slightly negative εNd and ISr values (0.703–0.704) suggest that intrusions originated from an enriched mantle reservoir. Low 3He/4He ratios in accessory minerals (ilmenite and magnetite) indicate an upper mantle source. Large-scale correlations link the Fennoscandian Shield with the Superior and Wyoming cratons.
Structural and Thermal Analysis of Softwood Lignins from a Pressurized Hot Water Extraction Biorefinery Process and Modified Derivatives
Authors: Lagerquist Lucas , Pranovich Andrey , Sumerskii Ivan , von Schoultz Sebastian , Vähäsalo Lari , Willför Stefan , Eklund Patrik
Published: January 2019

In this work we have analyzed the pine and spruce softwood lignin fraction recovered from a novel pressurized hot water extraction pilot process. The lignin structure was characterized using multiple NMR techniques and the thermal properties were analyzed using thermal gravimetric analysis. Acetylated and selectively methylated derivatives were prepared, and their structure and properties were analyzed and compared to the unmodified lignin. The lignin had relatively high molar weight and low PDI values and even less polydisperse fractions could be obtained by fractionation based on solubility in i-PrOH. Condensation, especially at the 5-position, was detected in this sulphur-free technical lignin, which had been enriched with carbon compared to the milled wood lignin (MWL) sample of the same wood chips. An increase in phenolic and carboxylic groups was also detected, which makes the lignin accessible to chemical modification. The lignin was determined to be thermally stable up to (273–302 °C) based on its Tdst 95% value. Due to the thermal stability, low polydispersity, and possibility to tailor its chemical properties by modification of its hydroxyl groups, possible application areas for the lignin could be in polymeric blends, composites or in resins.
Chemical Composition and Antimicrobial Activity of Essential Oil from Phytolacca dodecandra Collected in Ethiopia
Authors: Matebie Wondwosen Abebe , Zhang Wanchang , Xie Guangbo
Published: January 2019

The essential oil from Phytolacca dodecandra, a traditional herb of Ethiopia, has been studied, including the chemical composition and antimicrobial activity. The difference between four P. dodecandra samples (P-1–P-4), which differed in gender or location, has also been analyzed. The essential oils were obtained by steam distillation, while the aromas were extracted by head space solid-phase microextraction (HS-SPME) and both were analyzed by gas chromatography- mass spectrometry (GC-MS). The oils’ antimicrobial activities were evaluated by the microdilution method against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans. Ninety one components, representing 88.37 to 94.01% of the aromas, were identified. The compositions of the aromas of four samples are mainly dominated by aldehydes and ketones: 2-nonanone (1.80–30.80%), benzaldehyde (4.99–25.99%), and sulcatone (2.34–5.87%). Sixty components representing 64.61 to 69.64% of the oils were identified, and phytone (3.04–21.23%), phytol (4.11–26.29%) and palmitic acid (1.49–23.87%) are the major compounds. No obvious antimicrobial activity was observed for all the four essential oils.
Hwangryunhaedok-Tang Exerts Neuropreventive Effect on Memory Impairment by Reducing Cholinergic System Dysfunction and Inflammatory Response in a Vascular Dementia Rat Model
Authors: Sohn Eunjin , Kim Yu Jin , Lim Hye-Sun , Kim Bu-Yeo , Jeong Soo-Jin
Published: January 2019

Hwangryunhaedok-tang (HRT) is a traditional oriental herbal formula used in Asian countries for treating inflammatory diseases and controlling fever. Our present study aimed to determine whether HRT has therapeutic effects for patients with vascular dementia (VaD) using a bilateral common carotid artery occlusion (BCCAO) rat model and assessing spatial memory impairment and activation of neuroinflammation. BCCAO was performed in male Sprague Dawley rats to induce VaD, and oral HRT was administered daily for 30 d. Our data showed that HRT ameliorated BCCAO-induced memory and cognitive impairment in behavioral tests. In addition, HRT reversed cholinergic dysfunction and neuronal damage in the hippocampus of BCCAO rats. Furthermore, HRT attenuated microglial activation and reduced the phosphorylation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase (JNK) induced by BCCAO. Simultaneous high-performance liquid chromatography analysis of HRT using index compounds from the herbal composition revealed that both HRT ethanol extract and commercial HRT granules primarily comprise geniposide, baicalin, and berberine. Our study showed that HRT administration resulted in the prevention of neuronal injury induced by BCCAO through improvement of cholinergic dysfunction and inhibition of neuroinflammatory responses, suggesting that HRT may have potential as a treatment for VaD.
Application of pH-Responsive Fucoidan/Chitosan Nanoparticles to Improve Oral Quercetin Delivery
Authors: Barbosa Ana Isabel , Costa Lima Sofia A. , Reis Salette
Published: January 2019

Polymeric nanoparticles based on fucoidan and chitosan were developed to deliver quercetin as a novel functional food. Through the polyelectrolyte self-assembly method, fucoidan/chitosan (F/C) nanoparticles were obtained with three different weight ratios (1/1, 3/1, and 5/1). The content of quercetin in the fucoidan/chitosan nanoparticles was in the range 110 ± 3 to 335 ± 4 mg·mL−1, with the increase of weight ratio of fucoidan to chitosan in the nanoparticle. Physicochemically stable nanoparticles were obtained with a particle size within the 300–400 nm range and surface potential higher than +30 mV for the 1F/1C ratio nanoparticle and around −30 mV for the 3F/1C and 5F/1C ratios nanoparticles. The 1F/1C ratio nanoparticle became larger and more unstable as the pH increased from 2.5 to 7.4, while the 3F/1C and 5F/1C nanoparticles retained their initial characteristics. This result indicates that the latter nanoparticles were stable along the gastrointestinal tract. The quercetin-loaded fucoidan/chitosan nanoparticles showed strong antioxidant activity and controlled release under simulated gastrointestinal environments (in particular for the 3F/1C and 5F/1C ratios), preventing quercetin degradation and increasing its oral bioavailability.
Acknowledgement to Reviewers of Methods and Protocols in 2018
Authors:   MPs Editorial Office
Published: January 2019

Rigorous peer-review is the corner-stone of high-quality academic publishing [...]
Quantum Dot Doping-Induced Photoluminescence for Facile, Label-Free, and Sensitive Pyrophosphatase Activity Assay and Inhibitor Screening
Authors: Tian Yishen , Hao Lijie , Wang Chao , Yang Xiaoyan , Liu Shufeng
Published: January 2019

Development of simple, convenient, and sensitive assay methods for pyrophosphatase (PPase) activity is of importance, for disease diagnosis and drug discovery. Herein, a simple, rapid, label-free, and sensitive fluorescence sensor for PPase activity assay is developed, using Cu2+ doping-induced quantum dot (QD) photoluminescence as a signal reporter. The Cu2+ doping of ZnSe QD can induce a dopant-dependent emission response, which will be inhibited after the premixing of Cu2+ with pyrophosphate (PPi), to form a Cu2+-PPi complex. Then, the hydrolysis of PPi into phosphate (Pi), specifically catalyzed by PPase, liberates the free Cu2+ to regain the QD doping for the fluorescence response, which is highly dependent on the PPase activity. The PPase can be sensitively and selectively assayed, with a detection limit of 0.1 mU/mL. The developed sensing strategy can be also employed for the PPase inhibitor screening. Thus, the current QD doping-based sensing strategy offers an efficient and promising avenue for Cu2+, PPi, or PPase-related target analysis, and might hold great potential for the further applications in the clinical disease diagnosis.
Bi-Metal Phosphide NiCoP: An Enhanced Catalyst for the Reduction of 4-Nitrophenol
Authors: Sun Lijie , Xiang Xia , Wu Juwei , Cai Chao , Ao Dongyi , Luo Jinling , Tian Chengxiang , Zu Xiaotao
Published: January 2019

Porous phosphide NixCoyP composite nanomaterials are successfully synthesized at different Ni/Co ratios (=0, 0.5, 1, and 2) to reduce 4-nitrophenol. The X-ray diffraction and X-ray photoelectron spectroscopy results demonstrate that the products are CoP, NiCoP/CoP, NiCoP, and NiCoP/Ni2P when the Ni/Co ratio is 0, 0.5, 1, and 2, respectively. The products exhibit different catalytic performance for reduction of 4-nitrophenol at room temperature. Among them, the pure NiCoP delivers a better catalytic efficiency with k app = 677.4 × 10 − 2   min − 1 and k = 338.7   ( Lg − 1 min − 1 ) , due to the synergy between Ni and Co atoms. The sequence of catalytic efficiency of different samples is CoP < NiCoP/CoP < NiCoP/Ni2P < NiCoP.
Facile Electrochemical Sensor for Nanomolar Rutin Detection Based on Magnetite Nanoparticles and Reduced Graphene Oxide Decorated Electrode
Authors: He Quanguo , Wu Yiyong , Tian Yaling , Li Guangli , Liu Jun , Deng Peihong , Chen Dongchu
Published: January 2019

A new electrochemical sensor for nanomolar rutin detection based on amine-functionalized Fe3O4 nanoparticles and electrochemically reduced graphene oxide nanocomposite modified glassy carbon electrode (NH2-Fe3O4 NPs-ErGO/GCE) was fabricated through a simple method, and the X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), transmission electron microscope (TEM), vibrating sample magnetometer (VSM) and electrochemical technique were used to characterize the modified electrode. The electrochemical behavior of rutin on the Fe3O4 NPs-ErGO/GCE was studied in detail. The electrochemical response of rutin at this modified electrode was remarkably higher than that of the bare GCE or other modified GCE (GO/GCE, Fe3O4 NPs-GO/GCE, and ErGO/GCE). Under the optimum determination conditions, Fe3O4 NPs-ErGO/GCE provided rutin with a broader detection range of 6.0 nM–0.1 µM; 0.1–8.0 µM and 8.0–80 µM, a minimum detectable concentration of 4.0 nM was obtained after 210 s accumulation. This novel method was applied in determination of rutin in pharmaceutical tablets and urine samples with satisfactory results.
Facile Preparation of Self-Assembled Polydopamine-Modified Electrospun Fibers for Highly Effective Removal of Organic Dyes
Authors: Wang Cuiru , Yin Juanjuan , Wang Ran , Jiao Tifeng , Huang Haiming , Zhou Jingxin , Zhang Lexin , Peng Qiuming
Published: January 2019

Polydopamine (PDA) nanoparticles can be used as an adsorbent with excellent adsorption capacity. However, nanosized adsorbents are prone to aggregation and thus are severely limited in the field of adsorption. In order to solve this problem, we utilized polydopamine in-situ oxidation self-polymerization on the surface of polycaprolactone (PCL)/polyethylene oxide (PEO) electrospun fiber after solvent vapor annealing (SVA) treatment, and successfully designed and prepared a PCL/PEO@PDA composite membrane. The SVA treatment regulated the microscopic morphology of smooth PCL/PEO electrospun fibers that exhibited a pleated microstructure, increasing the specific surface area, and providing abundant active sites for the anchoring of PDA nanoparticles. The PCL/PEO@PDA composite obtained by chemical modification of PDA demonstrated numerous active sites for the adsorption of methylene (MB) and methyl orange (MO). In addition, the PCL/PEO@PDA composites were reusable several times with good reutilization as adsorbents. Therefore, we have developed a highly efficient and non-agglomerated dye adsorbent that exhibits potential large-scale application in dye removal and wastewater purification.
Composite Magnetic Photocatalyst Bi5O7I/MnxZn1−xFe2O4: Hydrothermal-Roasting Preparation and Excellent Photocatalytic Activity
Authors: Wang Hailong , Xu Longjun , Liu Chenglun , Lu Yuan , Feng Qi , Wu Tingzeng , Wang Ruiqi
Published: January 2019

A new composite magnetic photocatalyst, Bi5O7I/MnxZn1−xFe2O4, prepared by a hydrothermal-roasting method was studied. The photocatalytic properties of Bi5O7I/MnxZn1−xFe2O4 were evaluated by degradation of Rhodamine B (RhB) under simulated sunlight irradiation, and the structures and properties were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), ultraviolet-visible light (UV-Vis) diffuse reflectance spectra (DRS), and a vibrating sample magnetometer (VSM). The results indicated that Bi5O7I/MnxZn1−xFe2O4 was an orthorhombic crystal, which was similar to that observed for Bi5O7I. Bi5O7I/MnxZn1−xFe2O4 consisted of irregularly shaped nanosheets that were 40–60 nm thick. The most probable pore size was 24.1 nm and the specific surface area was 7.07 m2/g. Bi5O7I/MnxZn1−xFe2O4 could absorb both ultraviolet and visible light, and the energy gap value was 3.22 eV. The saturation magnetization, coercivity and residual magnetization of Bi5O7I/MnxZn1−xFe2O4 were 3.9 emu/g, 126.6 Oe, and 0.7 emu/g respectively, which could help Bi5O7I/MnxZn1−xFe2O4 be separated and recycled from wastewater under the action of an external magnetic field. The recycling experiments revealed that the average recovery rate of the photocatalyst was 90.1%, and the photocatalytic activity was still more than 81.1% after five cycles.
Wheat Albumin Increases the Ratio of Fat to Carbohydrate Oxidation during the Night in Healthy Participants: A Randomized Controlled Trial
Authors: Saito Shinichiro , Sakuda Toshitaka , Shudo Aiko , Sugiura Yoko , Osaki Noriko
Published: January 2019

Not only are energy expenditure (EE) and the respiratory quotient (RQ) parameters of the energy nutrient utilization and energy balance, they are also related to the development of obesity. In this study, post-meal night-time energy metabolism was investigated following the oral ingestion of wheat albumin (WA) with a late evening meal. A randomly assigned, double-blind, placebo-controlled crossover trial for a single oral ingestion in healthy participants was completed. The participants ingested the placebo (PL) or WA (1.5 g) containing tablets 3 minutes before the late evening meal at 22:00 hour, and energy metabolism was measured using a whole-room indirect calorie meter until wake-up. The participants were in bed from 00:00 hour until 06:30 hour. Twenty healthy participants completed the trial and were included in the analyses. Night-time RQ and carbohydrate oxidation were significantly lower following the WA treatment as compared with the PL treatment. Although the total EE was not significantly different between treatments, postprandial fat oxidation was significantly higher following the WA treatment as compared with the PL treatment. In conclusion, WA has the potential to shift the energy balance to a higher ratio of fat to carbohydrate oxidation during the night.