Contribution of mitochondrial therapy to processes of regeneration and selforganization of the cell system of the resected liver in rats
Authors: O.I. Kit, , A.I. Shikhlyarova, , E.M. Frantsiyants, , I.V. Neskubina, , I.V. Kaplieva, , V.A. Bandovkina, , M.A. Engibaryan, , Y.V. Przhdetskiy, , L.K. Trepitaki, , Y.A. Pogorelova, , G.V. Zhukova, , I.A. Popov, , N.D. Cheryarina, , S.V. Shlyk, , I.M. Kotieva, , L.P. Kuchkina, , R.G. Luganskaya, , V.A. Kovalenko, 
Published: December 2022

Topicality. Studies of the processes of the regeneration in the liver are not new, but however the development of the technologies of bio-therapy is capable of shifting the conventional approaches to the regeneration of the lost tissues. Mitochondrial therapy (MCT) occupies one of the highly important places in the list of bio-technologies by offering some unique mechanisms of the restoration of the organs. The liver having its specific tissue property of the regeneration demonstrates upon completion of hepatoectomy mitochondrial insufficiency and inhibition of the regenerative processes. Therefore it is required to evaluate the efficacy of mitochondrial stimulation in an experimental model in vivo with the proper morphological confirmation of the productivity and acceleration of the regenerative function of the liver that has become decisive for our research study work. Materials and methods. We have resected in 30 albino outbred male rats the left lobe of the liver, which is the maximum tolerable volume, amounting to 70% of the total mass of this organ, and upon of expiration of 24 hours we have transplanted to them intraperitoneally mitochondria harvested from a donor rat. Mitochondria have been isolated with the use of the differential centrifugation with high-speed refrigerated centrifuge Avanti J-E, BECMAN COULTER, USA. The morphological ORIGINAL RESEARCH examination of the liver sections has been conducted after their embedding in paraffin wax and staining with hematoxylin-eosin using microscopic testing of the liver sections with Axiovert (Carl 44 Zeiss, Germany) and applying the visualization software Axiovision 4 (Carl Zeiss, Germany). Results and discussion. It has been established that within the same period of time the quantitative data on the mass of the liver and its stump after MCT have exceeded the respective reference data by a factor of 1,6 (р˂0,05). The efficacy of the MCT application with respect to the regenerative function of the liver has been confirmed by the quality of the hepatic self-organization and replenishment of the key structures. A considerable supply of the most important cell elements of the connective tissue to the capsulated zone of the stump of the liver, an activation of neo-angiogenesis, hyperplasia and hypertrophy of hepatocytes with the repetitive order of the arrangement of the lobe structure of full value are manifestations of the acceleration of the regeneration. Conclusion. The high energy potential of fresh mitochondria makes possible to realize the trigger mechanisms of the bio-synthetic processes addressing an immediate restoration of the damaged or disordered liver tissues that can hold the greatest promise in emergency surgery and the activation of slowly progressive processes.