bluuebunny/crossref_metadata_2025 huggingface dataset:
Criminal History, Race, and Housing Type: An Experimental Audit of Housing Outcomes
Authors: Leasure Peter , Doyle R. Caleb , Boehme Hunter M. , Zhang Gary
Published: March 2022

Although there are a number of experimental studies showing that private housing can be difficult to secure for those with criminal history, many issues in this area remain unexplored or underexplored. The goal of the current study was to address the following unexplored or underexplored issues: (a) the impact of various types of multiple conviction records on private housing outcomes, including one that possessed a certificate of relief; (b) racial differences in private housing outcomes; (c) the impact of housing type on private housing outcomes; and (d) the impact of a criminal record, race, and housing type interaction on private housing outcomes. This goal was achieved with the use of a field experiment (correspondence audit). Results showed several statistically and substantively significant differences among the criminal record, race, and housing type conditions. These results can be used to better inform individuals with criminal history who are seeking private housing options.
Simulation and optimization of mining-separating-backfilling integrated coal mine production logistics system
Authors: Wang Xiang-Qian , Long Shuang-Shuang , Meng Xiang-Rui
Published: March 2022

As a new collaborative mining technology, the integration of mining-separating-backfilling (IMSB) enables the coal industry to realize safe, efficient, and green mining of coal resources at the expense of making the underground coal mine production more complex. In coal mining, the primary problem is to eliminate the production logistics system bottleneck to increase coal output. The key to synergetic mining is to realize that the mining capacity of the fully mechanized face should match the underground raw coal separating and gangue backfilling capacities. Considering a coal mine with IMSB in Henan Province, AnyLogic simulation software was used to simulate and optimize the production logistics system based on a generalized stochastic Petri net (GSPN). The main simulation results show that: (1) the raw coal separating capacity of this deep mine matches its mining capacity, and the gangue backfilling capacity can almost meet the demand for backfilling after raw coal mining; (2) belt conveyors 1 and 6 are the transportation bottlenecks of increasing production in the mine, and the lifting capacity of the main shaft is insufficient; (3) after optimization, the clean coal output of this coal mine increased by 3087 t monthly. This research promotes synergetic mining in the coal industry and can serve as a reference for the optimization of similar coal mine production logistics systems.
Evaluation and Management of Tinnitus: Are There Opportunities for Improvement?
Authors: Grundfast Kenneth M. , Jamil Taylor L.
Published: March 2022

Objective

To review current information about diagnosis and management of tinnitus aiming to identify opportunities for achieving a cost‐effective, efficient, evidence‐based approach that meets the needs of tinnitus sufferers.

Data Sources

PubMed/MEDLINE.

Review Methods

In total, 249 relevant published reports were reviewed. Pertinent keywords and MeSH terms identified reports via PubMed and EMBASE. Acknowledged experts were consulted on ways to improve tinnitus management.

Conclusions

There may be opportunities to improve evaluation and management of patients with tinnitus using modern modes of communication and a multidisciplinary therapeutic approach.

Implications for Practice

Tinnitus can adversely affect quality of life while being time‐consuming and costly to evaluate and manage. Based on both personal experience and the reports of others, patients with tinnitus who choose to see a physician primarily want to know two things: (1) that the tinnitus that is so distressing will not remain at the same level of severity forever and (2) that something can be done to help cope with the tinnitus that is so annoying. Recent advancements in internet communications, social media, information technology, artificial intelligence, machine learning, holistic medical care, mind‐body integrative health care, and multidisciplinary approaches in medical therapeutics may be possibly making new ways of meeting the needs of patients with tinnitus.
Health Technology Assessment in India: Necessity, Progress and Future Plans
Authors: Bhatia Hardik , Dharmagadda Sreedhar
Published: March 2022

The implementation of a universal healthcare coverage by the Indian government has forced a relook into the existing systems of healthcare decision-making. Health technology assessment (HTA) is being looked at to provide efficient decision-making and helping in priority setting for the policymakers. The Ministry of Health and Family Welfare has, therefore established a Medical Technology Assessment Board as a way of institutionalising the HTA framework and methodology in India. Despite some challenges, the future looks bright for HTA in India.
New in town, already settled in: Assessing the behavioural and experiential indicators that lead to acculturative advantages
Authors: Han Qin
Published: March 2022

We supplement extant literature on acculturation by introducing a new construct – individual acculturation action profile (IAAP) – consisting of a configuration of behavioural and experiential indicators that reflect an individual’s previous and current contact with and participation in other cultures. We operationalise each IAAP indicator individually, and the IAAP construct as an aggregated index (IAAPi), by assigning different weights to each construct indicator based on the magnitude of its theorised influence. We distinguish the antecedents of IAAP at multiple levels. Whilst contextual factors are likely to enhance or hinder people’s participation in other cultures, we propose a taxonomy that addresses the dynamism between context and individual initiative. This article thereby expands literature on acculturation, offering notable implications for advantageous acculturative processes and outcomes. The proposed operationalisation of the IAAP construct at the acculturation–organisation nexus can be applied to study many walks of society and outcomes at multiple levels.
Algebraic analysis and reconstruction of the Philips Pavilion’s hyperbolic paraboloid surfaces
Authors: Fischer Thomas , Wortmann Thomas
Published: March 2022

In this article, we present a procedure to derive algebraic descriptions from geometric descriptions of trimmed hyperbolic paraboloid (or ‘hypar’) surfaces. We contextualise this procedure historically, and we illustrate its application using the 1958 Philips Pavilion by Le Corbusier and Iannis Xenakis as a case study. The procedure uses parametric modelling and computational optimisation to converge on close algebraic approximations of hyperbolic paraboloid geometry through a successive breakdown of vast search spaces. It departs from coordinate data of three or four vertices of a geometrically described hyperbolic paraboloid and yields the surface’s two quadratic coefficients, the coordinates of its centroid location and the rotation angles of its spatial orientation. The procedure exemplifies the under-explored analytical (as opposed to generative) use of computational optimisation and parametric modelling in the field of architectural computing.
Factors affecting minimal manifestation status induction in myasthenia gravis
Authors: Li Yi , Yang Shumei , Dong Xiaohua , Li Zhibin , Peng Yuyao , Jin Wanlin , Chen Di , Zhou Ran , Jiang Fei , Yan Chengkai , Yang Huan
Published: March 2022

Background:

Minimal manifestation status (MMS) is an important landmark in the treatment of myasthenia gravis (MG), and predictors of MMS induction have rarely been identified in previous studies.

Objective:

The objective of this study is to evaluate the clinical factors associated with MMS induction among patients with MG.

Design:

This two-step retrospective cohort study with a single center investigated the factors that may be associated with MMS induction and retested these predictors in a test cohort.

Methods:

A total of 388 diagnosed MG patients who visited Xiangya Hospital between 1 July 2015 and 1 July 2019 were involved. We performed detailed chart reviews and recorded all cases achieving MMS. Demographics and clinical characteristics were also collected and their relationships to achieving MMS were investigated.

Results:

MMS was achieved in 124 patients (50.2%), and the median time to achieve MMS was 26 months. Several factors were found to be associated with MMS induction in exploring cohort, including muscle-specific tyrosine-protein kinase receptor (MuSK) antibody positivity (adjusted hazard ratio, HR = 4.333, 95% confidence interval, CI: 1.862–10.082), isolated ocular involvement (adjusted HR = 1.95, 95% CI: 1.284–2.961), and low baseline quantitative myasthenia gravis score (QMG score; adjusted HR = 2.022, 95% CI: 1.086–3.764). These factors were then retested in the test cohort. Isolated ocular involvement or low baseline QMG scores were factors found to be beneficial for MMS induction were confirmed.

Conclusion:

Isolated ocular involvement and low baseline QMG score are predictors of MMS induction in MG patients.
The effects of the COVID-19 pandemic, risk perception, and perceived social support on public trust in physicians in China: A latent transition analysis
Authors: Chen Yidi , Hall Brian J. , Li Wenju , Wu Jian hui , Ma Jinjin , Zhu, Huanya , Gan Yiqun
Published: March 2022

A population-based, longitudinal study was conducted among 29 provinces in mainland China to investigate how public trust in physicians (PTP) changed since the outbreak of COVID-19 and how the resulting lockdown and social support contributed to its restoration. The baseline sample ( n = 3,233) was obtained during the period of the most rapid progression of COVID-19 (February 1 to 9, 2020, T1). Follow-up ( n = 1,380) took place during the recovery period (March 17 to 24, T2). Latent profile models and a latent transition model were estimated. Participants were classified into either a moderate trust (21% at T1; 45% transition into high at T2) or a high trust (79% at T1; 88% remained in the high group) group in the latent profile. A latent transition from moderate to high trust was observed in locked-down regions and among those with higher social support. Social support moderated the transition from low to high trust. The current study showed that the epidemic outbreak and lockdown experience in China were associated with increased PTP; furthermore, public trust can be restored during a public health emergency. Attention should be paid to assure that social support and risk management strategies maintain PTP.
Equitable Identification of Underrepresented Gifted Students: The Relationship Between Students’ Academic Achievement and a Teacher-Rating Scale
Authors: Lee Hyeseong , Seward Kristen , Gentry Marcia
Published: March 2022

Underrepresentation of students from low-income families and/or certain ethnic groups has been a persistent worldwide problem in the field of gifted education. Because teachers frequently serve as gatekeepers for students’ participation in gifted programming, their roles in the identification process are critical. In this study, 55 elementary school teachers in South Korea completed a teacher-rating scale (HOPE Scale; Peters & Gentry, 2012a) for all students ( n = 1,157) in their classrooms, including their evaluation of each students’ reading and mathematics talents. Their ratings and talent evaluations were then compared to students’ reading and mathematics achievement scores to determine whether teachers’ ratings promoted more equitable identification of students from underrepresented groups. In addition, we explored the effects of combining various cut-off scores for each assessment for the identification of students from low-income and/or culturally diverse families.
Comparison of Efficacy and Safety of Hybrid Arch Bar with Erich Arch Bar in the Management of Mandibular Fractures: A Randomized Clinical Trial
Authors: Sankar Hariram , Rai Sachin , Jolly Satnam S. , Rattan Vidya
Published: March 2022

Study Design

A clinical randomized control trial.

Objective

To compare the efficacy and safety of Hybrid arch bar (HAB) with Erich arch bar (EAB) in fracture management of the mandible.

Methods

In this randomized clinical trial, 44 patients were divided into 2 groups:- Group 1, N = 23 (EAB group) and Group 2, N = 21 (HAB group). The primary outcome was time taken for the application of arch bar, while the inner and outer glove puncture, operator prick, oral hygiene, arch bar stability, complications of HAB, and cost comparison were secondary outcomes.

Results

The time taken for the application of arch bar in group 2 was significantly shorter than group 1 (55.66 ± 17.869 min vs 82.04 ± 12.197 min) and the frequency of outer glove puncture was also significantly lesser for group 2 (0 punctures vs 9 punctures). Better oral hygiene was found in group 2. EAB was cost-effective than HAB (Rs 700 ± 239.79 vs Rs 1742.50 ± 257.14). The stability of the arch bar was comparable in both groups. Group 2 had associated complications of root injury in 2 out of 252 screws placed and the screw head got covered by soft tissue in 137 out of 252 screws placed.

Conclusions

Thus, HAB was better than EAB with a shorter time of application, less risk of prick injury, and improved oral hygiene. Clinical trial registry name- clinical trials registry- India, URL- http://ctri.nic.in , registration number- CTRI/2020/06/025966.
Safety and Efficacy of Anterior Cervical Discectomy and Fusion with Uncinate Process Resection: A Systematic Review and Meta-Analysis
Authors: Yin Mengchen , Ding Xing , Zhu Yuefeng , Lin Rui , Sun Yueli , Xiao Yu , Wang Tao , Yan Yinjie , Ma Junming , Mo Wen
Published: March 2022

Study design

This is a meta-analysis and systematic review of the available literature.

Objective

In the case of severe foraminal stenosis, conducting uncinate process resection (UPR) during ACDF could achieve complete nerve root decompression and significant relief of neurological symptoms for CR. However, there is some controversy regarding its necessity and safety. This study aims to compare the safety and efficacy of ACDF with UPR and ACDF.

Methods

The following electronic databases were searched: Medline, PubMed, Embase, the Cochrane Central Register of Controlled Trials, Evidence Based Medicine Reviews, VIP, and CNKI. And the following data items were considered: baseline demographics, efficacy evaluation indicators, radiographic outcome, and surgical details.

Results

10 studies were finally identified, including 746 patients who underwent ACDF with UPR compared to 729 patients who underwent ACDF. The group of ACDF with UPR had statistically longer intraoperative time (95% CI: 4.83, 19.77, P = .001) and more intraoperative blood loss (95% CI: 12.23, 17.76, P < .001). ACDF with UPR obtained a significantly better improvement of Arm VAS at postoperative first follow-up (95% CI: −1.85, −.14 P = .02). There was no significant difference found in improvement of Neck VAS at postoperative latest follow-up (95% CI: −.88, .27, P = .30), improvement of Arm VAS at postoperative latest follow-up (95% CI: −.59, −.01, P = .05), improvement of NDI (95% CI: −2.34, .33, P = .14), JOA (95% CI: −.24, .43, P = .56), change of C2-C7 lordosis (95% CI: −.87, 1.33, P = .68), C2-C7 SVA (95% CI: −.73, 5.08, P = .14), T1 slope (95% CI: −2.25, 1.51, P = .70), and fusion rate (95% CI: .83, 1.90 P = .29).

Conclusion

ACDF with UPR is an effective and necessary surgical method for CR patients with severe foraminal stenosis.
Innovation in Resident Selection: Life Without Step 1
Authors: Patel Hares , Yakkanti Ram , Bellam Krishna , Agyeman Kofi , Aiyer Amiethab
Published: March 2022

INTRODUCTION

The announcement of Step 1 shifting to a Pass/Fail metric has prompted resident selection committees (RSCs) to pursue objective methods of evaluating prospective residents. Regardless of the program's specialty or affiliated hospital/school, RSCs universally aim to recognize and choose applicants who are an “optimal fit” to their programs.1 An optimal fit can be defined as a candidate who thrives in the clinical and academic setting, both contributing to and benefiting from their respective training environments.

OBJECTIVE

The objective of this scoping review is to evaluate alternative, innovative methods by which RSCs can evaluate applicants and predict success during residency. Objective methods include: Step 2 scores, Traditionally Used Metrics (core clerkship scores), interview performance, musical talent, sports involvement, AOA membership, research publications, unprofessional behavior, Dean's letters, Rank list, judgement testing, and specialty-specific shelf exams.13–15

METHODS

A scoping review was performed in compliance with the guidelines indicated by the PRISMA Protocol for scoping review.18 9308 results were identified in the original PubMed search for articles with the key words “Resident Success”. Abstract screening and application of inclusion and exclusion criteria yielded 97 articles that were critically appraised via review of full manuscript.

RESULTS

Of the articles that focused on personality traits, situational judgement testing, and specialty specific pre-assessment, all of them demonstrated some level of predictability for resident success. Standardized Letter of Recommendations, Traditionally Used Metrics, and STEP 2 did not show a unanimous consensus in demonstrating predictability of a resident's success, this is because some articles suggested predictability and some articles disputed predictability.

CONCLUSION

The authors found personality traits, situational judgement testing, and specialty specific assessments to be predictive in selecting successful residents. Further research should aim to analyze exactly how RSCs utilize these assessment tools to aid in screening their large and competitive applicant pools to find residents that will be successful in their program.
Clapping Along or Clapping Back: Of the Discourse of Hijra Community’s Resilient Happiness in Y-Film’s Hum Hain Happy
Authors: Subramanian Prerna
Published: March 2022

Eva Illouz, whilst discussing her text Manufacturing Happy Citizens in an interview, points out that happiness in a neoliberal economy has become a way to measure our self-worth especially as it is individualised. Being happy, thus, means to be able to strive despite the odds and yet be optimistic: to be happy in a neoliberal setup is to be resilient. Indeed, resilience means bouncing back and putting your best foot forward in the gravest of circumstances, showing strength when things are against you: to be happy despite the odds. Keeping this aspect of resilience in mind, this essay is interested in broaching a more specific question: what happens when a structurally marginalised group is called resilient—when value of the group is solely located in its capacity to bounce back and remain within and not resist status-quo? The structurally marginalised group I will be referring to is the hijra community in India and I will discuss the idea of happiness in a video produced by Y films, the youth division of one of India’s biggest film production houses: Yash Raj films. The music video produced in 2016 is named ‘Hum Hain Happy (We are happy)’, a Hindi rendition of Pharrell William’s famous song of the same name, features six hijra community members from the city of Mumbai. The essay here offers a ‘friendly critique’ of the video’s efforts towards mainstreaming trans inclusion in representational practices which romanticise the struggle of the marginalised by valorising resilience as an inspirational response to systemic inequality. My critique, thus, does not invalidate representational practices in total but calls for looking at the dominant logics through which such representation is rendered possible. In so doing, I look at how the video’s narrative serves to quell collective resistance by translating individual resilience within status quo as happiness. On one hand, the makers of the video see the oppressed as valuable and respectable only in term of their resilient happiness and relatedly, by pedestalising this resilience as inspirational, essentialise/naturalise systemic inequalities and conditions of struggle. Anything that deters from this pedestal then is naturally seen as a negative response and not aspirational/inspirational. The essay first contextualises the video in the contemporary conditions of transgender rights in India and then proceeds to analyse its content regarding the narrative of resilience which obscures the conditions of transgender struggles in India, while elevating their positive attitude as inspirational, effectively obscuring the realities and possibilities of righteous rage and resistance.
Monitoring the safety of influenza A/H1N1 pandemic and seasonal vaccines in Morocco
Authors: Tebaa Amina , Benkirane Raja , Alj Loubna , Cherkaoui Imad , Soulaymani-Bencheikh Rachida
Published: March 2022

Background:

A vaccination campaign against pandemic influenza A/H1N1 was implemented in Morocco between November 2009 and April 2010. Overall, 705,883 subjects were vaccinated by Pandemrix, Arepanrix, and Panenza. The adverse events following immunization (AEFIs) data comparison was made with the 2014/2015 seasonal influenza vaccination campaign that was specifically investigated.

Aim:

To evaluate the safety of the 2009 pandemic influenza A/H1N1 vaccine and to compare it to that of 2014 seasonal influenza vaccine.

Methods:

During the pandemic vaccination campaign, the Morocco Pharmacovigilance Centre reinforced passive AEFI surveillance with an active and prospective monitoring programme of 1000 immunized people over 6 months at 10 randomly selected vaccination centres. For the 2014/2015 seasonal vaccination campaign, AEFI data were collected from spontaneous notifications.

Results:

Active monitoring of 2009 pandemic collected 771 AEFI reports, corresponding to an AEFI incidence rate of 77.1% with vaccination by either Pandemrix or Arepanrix vaccine in 95% of cases. Reported AEFI were most frequently local (37.7%), general (29.5%), and neurological reactions (20.3%). Most of the AEFI (95.5%) were observed during the first 48 hours after vaccination, and the remainder within 2 weeks. None of the reported AEFI were serious case. The highest rate of notification was documented for health professionals, followed by patients with diabetes or chronic respiratory diseases. Concerning passive surveillance, the AEFI notification rate was significantly higher for the 2009/2010 pandemic vaccine (3.1 vs 1.2 per 10,000). However, there was no significant difference between pandemic and seasonal vaccination with regards to the serious adverse events (SAE) notification rate (0.3 vs 0.2 per 10,000).

Conclusion:

Data analysis indicates that the vaccines used against 2009 pandemic influenza in Morocco have a satisfactory safety profile, similar to the seasonal influenza vaccine with the exception of local reactions as observed previously in other countries.
Democratizing Work: Redistributing power in organizations for a democratic and sustainable future
Authors: Battilana Julie , Yen Julie , Ferreras Isabelle , Ramarajan Lakshmi
Published: March 2022

Environmental destruction and social inequalities are increasingly urgent challenges. How can corporations, which have played a key role in creating and reproducing these problems, be part of the solution? In this paper, we advance that a shift to more democratic forms of organizing within corporations may be an important part of this transition. We first review scholarship on the disempowerment of workers. We then make the case for democratizing organizations, arguing that workers need to participate in firm decision-making so they can protect their rights and interests. We further suggest that democratic organizing practices may enable corporations to successfully pursue social and environmental objectives alongside financial ones, which is also important for addressing societal challenges. We then propose a research agenda for studying the democratization of organizations and its implications. In doing so, we highlight how organization scholars can build on prior research on democratic forms of organizing and draw from extant social science research outside of mainstream management scholarship. We conclude by calling for research that will document, and help us better understand, what it takes to develop democratic and sustainable organizations and societies.
Interactive effect of socio-eco-demographic characteristics and perceived physical activity barriers on physical activity level among older adults
Authors: Arazi Hamid , Izadi Mani , Kabirian Hadis
Published: March 2022

Background

Studies examining associations of socio-eco-demographic characteristics with physical activity (PA) participation of older adults have produced inconsistent results. Perceived PA barriers may be a possible explanation for the mixed findings. Therefore, the purpose of this study was to examine the correlation of socio-eco-demographic (SED) characteristics with PA of older adults and the moderation effects of perceived barriers of PA.

Methods

Three hundred eighty-four older adults (≥ 60 years old) were recruited from public places in six different cities. Questions regarding socio-eco-demographic characteristics, PA, and perceived PA barriers were asked, in-person, by two examiners. Ordinal logistic regression models examined the association of socio-eco-demographic characteristics with subjectively measured PA, and the interactive effects of subscales of perceived PA barriers and socio-eco-demographic variables for PA outcomes.

Results

Significant main effects for PA outcomes were found for education and living status (P < 0.01) and college-educated individuals and those were living in their private houses reported higher PA. Also, 24 significant interactive effects of perceived PA barriers by socio-eco-demographic factors were found (P < 0.05). Significant moderation effects by all subscales of perceived PA barriers were observed for education and living status. The effect of age for the PA outcomes was moderated by “lack of time”, “fear of injury”, and “lack of skill”. Only “fear of injury” and “lack of time” moderated the effect of gender and marriage for outcome variable, respectively. The effect of employment was moderated by “lack of willpower”, “fear of injury”, “lack of skill” and “lack of resources”.

Conclusions

Novel evidence revealed that there are moderations by perceived PA barriers for the effect of almost all socio-eco-demographic characteristics. These findings highlight a need to consider older adults’ perspectives and perceptions, when it comes to establish policies for PA participation.
Excitation-Power-Dependent Upconversion Luminescence Competition in Single β-NaYbF4:Er Microcrystal Pumped at 808 nm
Authors: Yuan Maohui , Yang Zining , Yang Xu , Wang Linxuan , Wang Rui , Lan Sheng , Han Kai , Wang Hongyan , Xu Xiaojun
Published: March 2022

Controlling the upconversion luminescence (UCL) intensity ratio, especially pumped at 808 nm, is of fundamental importance in biological applications due to the water molecules exhibiting low absorption at this excitation wavelength. In this work, a series of β-NaYbF4:Er microrods were synthesized by a simple one-pot hydrothermal method and their intense green (545 nm) and red (650 nm) UCL were experimentally investigated based on the single-particle level under the excitation of 808 nm continuous-wave (CW) laser. Interestingly, the competition between the green and red UCL can be observed in highly Yb3+-doped microcrystals as the excitation intensity gradually increases, which leads to the UCL color changing from green to orange. However, the microcrystals doped with low Yb3+ concentration keep green color which is independent of the excitation power. Further investigations demonstrate that the cross-relaxation (CR) processes between Yb3+ and Er3+ ions result in the UCL competition.

Graphical Abstract
Computational analysis of cortical neuronal excitotoxicity in a large animal model of neonatal brain injury
Authors: Kratimenos Panagiotis , Vij Abhya , Vidva Robinson , Koutroulis Ioannis , Delivoria-Papadopoulos Maria , Gallo Vittorio , Sathyanesan Aaron
Published: March 2022

Background

Neonatal hypoxic brain injury is a major cause of intellectual and developmental disability. Hypoxia causes neuronal dysfunction and death in the developing cerebral cortex due to excitotoxic Ca2+-influx. In the translational piglet model of hypoxic encephalopathy, we have previously shown that hypoxia overactivates Ca2+/Calmodulin (CaM) signaling via Sarcoma (Src) kinase in cortical neurons, resulting in overexpression of proapoptotic genes. However, identifying the exact relationship between alterations in neuronal Ca2+-influx, molecular determinants of cell death, and the degree of hypoxia in a dynamic system represents a significant challenge.

Methods

We used experimental and computational methods to identify molecular events critical to the onset of excitotoxicity-induced apoptosis in the cerebral cortex of newborn piglets. We used 2–3-day-old piglets (normoxic [Nx], hypoxic [Hx], and hypoxic + Src-inhibitor-treatment [Hx+PP2] groups) for biochemical analysis of ATP production, Ca2+-influx, and Ca2+/CaM-dependent protein kinase kinase 2 (CaMKK2) expression. We then used SimBiology to build a computational model of the Ca2+/CaM-Src-kinase signaling cascade, simulating Nx, Hx, and Hx+PP2 conditions. To evaluate our model, we used Sobol variance decomposition, multiparametric global sensitivity analysis, and parameter scanning.

Results

Our model captures important molecular trends caused by hypoxia in the piglet brain. Incorporating the action of Src kinase inhibitor PP2 further validated our model and enabled predictive analysis of the effect of hypoxia on CaMKK2. We determined the impact of a feedback loop related to Src phosphorylation of NMDA receptors and activation kinetics of CaMKII. We also identified distinct modes of signaling wherein Ca2+ level alterations following Src kinase inhibition may not be a linear predictor of changes in Bax expression. Importantly, our model indicates that while pharmacological pre-treatment significantly reduces the onset of abnormal Ca2+-influx, there exists a window of intervention after hypoxia during which targeted modulation of Src-NMDAR interaction kinetics in combination with PP2 administration can reduce Ca2+-influx and Bax expression to similar levels as pre-treatment.

Conclusions

Our model identifies new dynamics of critical components in the Ca2+/CaM-Src signaling pathway leading to neuronal injury and provides a feasible framework for drug efficacy studies in translational models of neonatal brain injury for the prevention of intellectual and developmental disabilities.
E-cadherin expressions on bladder and its association with cancer progressivity: a retrospective cohort study
Authors: Tambunan Made Parulian , Saraswati Meilania , Umbas Rainy , Mochtar Chaidir Arif , Hamid Agus Rizal Ardy Hariandy
Published: March 2022

Background

Bladder cancer is characterized by high recurrence and progressivity. E-cadherin serves as one of the most important molecules involved in the epithelial cells’ cell-to-cell adherence, suggested to inhibit tumor cell progression. This study aims to investigate the association between the E-cadherin expressions with bladder cancer progressiveness in 3 years.

Methods

This study was a retrospective cohort study involving bladder cancer patients in Cipto Mangunkusumo Hospital, Jakarta. Diagnosis of bladder cancers was confirmed by histopathological and immunohistochemistry examination between 2011 and 2018, with both grading and staging determined by uropathologists and uro-oncologists. E-cadherin was examined through immunohistochemistry examination at the time of diagnosis. Data on demography, muscle invasion, clinical staging, grade, metastasis, multifocality, and recurrence were obtained from medical records and pathology reports. The association of E-cadherin expression to muscle invasion and non-muscle invasion bladder cancer was evaluated and statistically analyzed. Patients’ survival data were followed up by phone.

Results

Forty bladder cancer patients with a mean age of 60.05 ± 10.3 years were included. Most subjects had high E-cadherin expression (85%), muscle invasion (65%), high grade (65%), no metastasis (87.5%), multifocality (65%), and no recurrence (62.5%). Lower expression of E-cadherin was associated with the higher clinical stage (p < 0.02) and metastasis (p < 0.001). Patients with low E-cadherin expression showed worse cumulative survival than the high one (mean 32 months vs. 25 months, p = 0.13).

Conclusions

Low level of E-cadherin was associated with the higher risk of muscle invasion, clinical staging, histological grade, and risk of metastasis. Meanwhile, patients with the high level of E-cadherin showed a better three-year survival rate.
Whole exome sequencing of pediatric leukemia reveals a novel InDel within FLT-3 gene in AML patient from Mizo tribal population, Northeast India
Authors: Vanlallawma Andrew , Lallawmzuali Doris , Pautu Jeremy L. , Scaria Vinod , Sivasubbu Sridhar , Kumar Nachimuthu Senthil
Published: March 2022

Background

Leukemia is the most common type of cancer in pediatrics. Genomic mutations contribute towards the molecular mechanism of disease progression and also helps in diagnosis and prognosis. This is the first scientific mutational exploration in whole exome of pediatric leukemia patients from a cancer prone endogamous Mizo tribal population, Northeast India.

Result

Three non-synonymous exonic variants in NOTCH1 (p.V1699E), MUTYH (p.G143E) and PTPN11 (p.S502P) were found to be pathogenic. A novel in-frame insertion-deletion within the juxtamembrane domain of FLT3 (p.Tyr589_Tyr591delinsTrpAlaGlyAsp) was also observed.

Conclusion

These unique variants could have a potential mutational significance and these could be candidate genes in elucidating the possibility of predisposition to cancers within the population. This study merits further investigation for its role in diagnosis and prognosis and also suggests the need for population wide screening to identify unique mutations that might play a key role towards precision medicine.
Plastid retrograde regulation of miRNA expression in response to light stress
Authors: Barczak-Brzyżek Anna , Brzyżek Grzegorz , Koter Marek , Siedlecka Ewa , Gawroński Piotr , Filipecki Marcin
Published: March 2022

Background

MicroRNAs (miRNAs) are a class of endogenous noncoding RNAs that play a pivotal role in the regulation of plant development and responses to the surrounding environment. Despite the efforts made to elucidate their function in the adaptation of plants to many abiotic and biotic stresses, their role in high light (HL) stress is still vague. HL stress often arises upon plant exposure to full sunlight. Subsequent changes in nuclear gene expression are triggered by chloroplast-derived retrograde signals.

Results

In this study, we show that HL is involved in miRNA-dependent regulation inArabidopsis thalianarosettes. Microtranscriptomic screening revealed a limited number of miRNAs reacting to HL. To explain the miRNA regulation mechanisms at the different biogenesis stages, chemical and genetic approaches were applied. First, we tested the possible role of plastoquinone (PQ) redox changes using photosynthetic electron transport chain inhibitors. The results suggest that increased primary transcript abundance (pri-miRNAs) of HL-regulated miRNAs is dependent on signals upstream of PQ. This indicates that such signals may originate from photosystem II, which is the main singlet oxygen (1O2) source. Nevertheless, no changes in pri-miRNA expression upon a dark–light shift in the conditionalfluorescent(flu) mutant producing1O2were observed when compared to wild-type plants. Thus, we explored the1O2signaling pathway, which is initiated independently in HL and is related to β-carotene oxidation and production of volatile derivatives, such as β-cyclocitral (β-CC). Pri-miRNA induction by β-CC, which is a component of this1O2pathway, as well as an altered response in themethylene blue sensitivity 1(mbs1) mutant support the role of1O2signaling in miRNA regulation.

Conclusions

We show that light stress triggers changes in miRNA expression. This stress response may be regulated by reactive oxygen species (ROS)-related signaling. In conclusion, our results link ROS action to miRNA biogenesis, suggesting its contribution to inconsistent pri- and mature miRNA dynamics.
Phenotype correlation analysis and excellent germplasm screening of herb Bletilla Rchb. f. based on comprehensive evaluation from thirty-three geographic populations
Authors: Huang Junfeng , Yuan Fang , Zhou Ming , Huang Tianyue , Zhang Yanjun , Liang Qiong
Published: March 2022

Background

The Bletilla genus of Orchidaceae includes plants with great economic value, among which B. striata is the main traditional medicinal plant, and its pseudobulb, known as BaiJi, was first recorded in Shennong’s Classic of Materia Medica. However, there has been little systemic evaluation of the germplasm quality of Bletilla plants in China. In order to comprehensive evaluate the Bletilla resources in China and screen out the candidate phenotypic traits determining yield and/or quality of Bletilla, the variation of phenotypic indicators (pseudobulb, leaf, stem, inflorescence, flower) and active ingredients contents (polysaccharide, total phenolics and militarine) in different populations of B. striata and B. ochracea were investigated through 4 years’ common-garden experiment.

Results

There were abundant phenotypic variations and significant differences among different populations in the morphological phenotypes, pseudobulb weight and main active ingredient contents. AHBZ, HBLT and HBSN populations showed good prospects for industrial development, presenting higher quality in terms of yield and main active ingredient content. Pseudobulb yield, polysaccharide and total phenol content are positively correlated with phenotypic traits. Militarine content is negatively correlated with almost all indexes. Plant height, leaf width and stem diameter may be important indicators of potential excellent germplasms.

Conclusions

Bletilla is not strictly geoauthentic medicinal plants. B. ochracea could be accepted as an alternative resource to B. striata. The best harvest period of Bletilla is the third year after cultivation. Plant height, leaf width and stem diameter may be important indicators of potential excellent germplasms. These results provide important information required for the efficient screening and utilization of Bletilla germplasm resources.
Using group model building to frame the commercial determinants of dietary behaviour in adolescence – proposed methods for online system mapping workshops
Authors: Chavez-Ugalde Yanaina , Toumpakari Zoi , White Martin , De Vocht Frank , Jago Russell
Published: March 2022

Background

Group model building (GMB) is a participatory approach whereby diverse stakeholders can share their views about a problem to create a collective understanding of a complex system. In this article we report our methodological approach to adapt face-to-face GMB processes to an online format to explore the mechanisms by which commercial drivers influence adolescents’ dietary behaviour. We use our experiences to make recommendations on how online GMB could be delivered.

Methods

We planned, adapted, piloted, delivered, and evaluated a series of online GMB workshops with adolescents, policymakers and public health practitioners to create a system map of the commercial determinants of dietary behaviour in adolescence. We adapted face-to-face GMB workshops to a series of 3 online GMB workshops with 11 adolescents (16–18 years) living in the Southwest of England, and one GMB workshop with policymakers and public health practitioners.

Results

In our experience, adapting, and delivering GMB online is feasible, engaging, cost-saving and an enjoyable experience. Participants gave positive feedback in terms of engagement and enjoyment, and it allowed them to recognise different points of view about the same problem. Participants became familiarised with system thinking and system dynamics concepts, developed a shared understanding of a complex issue and portrayed it in a system map that depicted the most important factors in a causal structure as well as their interactions at various levels.

Conclusions

We recommend using process mapping to understand the overall GMB process in an online environment and piloting the workshops to test the timings and flow between online platforms. For facilitation and delivery, facilitators need to ensure they can create an inviting and engaging online environment, even for participants who decide to have their cameras off. Separating GMB activities into different workshops allowed participants to reflect on the problem being discussed and bring new ideas to subsequent workshops. Evaluating the workshops enabled us to build evidence on the trade-offs between the effectiveness, quality and efficiency of online GMB workshops, and how this might be enhanced to identify leverage points and achieve systemic changes in complex issues.

Ethical approval

The research was approved by the University of Bristol’s Faculty of Health Sciences Research Ethics Committee (Ref: 99,003) and written consent was received from all participants.
Efficacy and predictor of anti-TNFα agents in patients with intestinal Behçet's disease
Authors: Miyazaki Haruka , Watanabe Daisuke , Okamoto Norihiro , Tokunaga Eri , Ku Yuna , Takenaka Haruka , Hoshi Namiko , Ooi Makoto , Kodama Yuzo
Published: March 2022

Background

Behçet’s disease (BD) is a recurrent multisystem inflammatory disease. Anti-tumor necrosis factor (TNF) α agents have been used to treat patients with intestinal BD with severe disease activity or those who are resistant to conventional treatments; however, the long-term efficacy of anti-TNFα agents in intestinal BD remains unclear. In the present study, we investigated the clinical outcomes and predictors of discontinuation of anti-TNFα agents in patients with intestinal BD.

Methods

We reviewed the medical records of patients with intestinal BD who received first-line anti-TNFα agents between January 2009 and June 2020. The primary outcome was the percentage of patients who continued anti-TNFα therapy for 48 weeks. Secondary outcomes included the percentage of patients who achieved marked improvement, complete remission, and mucosal healing, as well as predictors of discontinuation of anti-TNFα agents.

Results

A total of 29 patients were included in the study. Twenty-two (75.9%) patients continued anti-TNFα therapy for 48 weeks. The percentage of patients who achieved marked improvement, complete remission, and mucosal healing at week 48 was 48.3%, 37.9%, and 48.3%, respectively. At week 96, 11 (37.9%) patients achieved marked improvement, complete remission, and mucosal healing. A higher C-reactive protein level (CRP; ≥ 1 mg/dL) at baseline was a predictor of discontinuation of anti-TNFα agents.

Conclusions

The 48-week continuation rate of anti-TNFα agents was 75.9% in bio-naïve patients with intestinal BD. However, a higher baseline CRP level (≥ 1 mg/dL) was associated with discontinuation of anti-TNFα agents.
The prevalence and prognostic value of frailty screening measures in patients undergoing surgery for colorectal cancer: observations from a systematic review
Authors: McGovern Josh , Dolan Ross D. , Horgan Paul G. , Laird Barry J. , McMillan Donald C.
Published: March 2022

Introduction

Frailty is a complex multifactorial syndrome characterised by a significant increase in vulnerability and worsened health outcomes. Despite a range of proposed frailty screening measures, the prevalence and prognostic value of frailty in patients undergoing surgery for colorectal cancer is not clear.

Aim

The aim of this present review was to examine the use of commonly employed frailty screening measures in patients undergoing surgery for colorectal cancer.

Methods

A systematic search of PubMed and Medline was carried out to identify studies reporting the use of frailty screening tools or measures in patients undergoing surgery for colorectal cancer. The screening measure used and prevalence of frailty within the population were recorded. Outcomes of interest were the incidence of post-operative complications, 30-day mortality and overall survival.

Results

Of the 15 studies included (n = 97, 898 patients), 9 studies were retrospective and included patients aged 70 years or older (n = 96, 120 patients). 5 of 12 studies reported that frailty was independently associated with the incidence of post-operative complications. There was also evidence that frailty was independently associated with 30-day mortality (1 of 4 studies, n = 9, 252 patients) and long-term survival (2 of 3 studies, n = 1, 420 patients).

Conclusions

Frailty was common in patients with colorectal cancer and the assessment of frailty may have prognostic value in patients undergoing surgery. However, the basis of the relationship between frailty and post-operative outcomes is not clear and merits further study.
Long-term association of vegetable and fruit intake with risk of dementia in Japanese older adults: the Hisayama study
Authors: Kimura Yasumi , Yoshida Daigo , Ohara Tomoyuki , Hata Jun , Honda Takanori , Hirakawa Yoichiro , Shibata Mao , Oishi Emi , Sakata Satoko , Furuta Yoshihiko , Chen Sanmei , Uchida Kazuhiro , Nakao Tomohiro , Kitazono Takanari , Ninomiya Toshiharu
Published: March 2022

Background

Several prospective Western studies have reported an inverse association of vegetable and fruit intake with dementia risk. However, there is limited epidemiologic evidence in Asians. This study investigated the association of intakes of vegetables, fruits, and their nutrients on the risk of incident dementia and its subtypes in a Japanese community.

Methods

A total of 1071 participants (452 men and 619 women) aged ≥60 years without dementia at baseline were prospectively followed up for 24 years. Intakes of vegetables, fruits, and nutrients were evaluated using a 70-item semiquantitative food frequency questionnaire at baseline and were categorized into quartiles separately by gender. The outcome measure was the development of dementia and its subtypes—namely, Alzheimer’s disease (AD) and vascular dementia (VaD). The risk estimates of incident dementia were computed using a Cox proportional hazards model.

Results

During the long-term follow-up period, 464 subjects developed dementia, of whom 286 had AD and 144 had VaD. Higher vegetable intake was associated gradually with lower risk of developing dementia and AD (both P-trend < 0.05), but not VaD, after adjusting for confounders. Subjects allocated the highest quartile of vegetable intake had 27 and 31% lower risk of dementia and AD, respectively, than those with the lowest quartile. The risk of dementia decreased significantly with higher intakes of vitamin A, riboflavin, vitamin C, magnesium, calcium, and potassium (all P-trend < 0.05). Subjects with higher total dietary fiber intake tended to be at decreased risk for total dementia (P-trend = 0.07). Meanwhile, there were no significant associations between fruit intake and the risk of dementia and its subtypes.

Conclusion

Higher intakes of vegetables and their constituent nutrients were associated with a lower risk of dementia in Japanese older adults. A diet rich in vegetables may be beneficial in reducing the dementia risk in Asians.