bluuebunny/crossref_metadata_2025 huggingface dataset:
New Automated Method for Lung Functional Volumes Delineation with Lung Perfusion PET/CT Imaging
Authors: Pinot Fanny , Bourhis David , Bourbonne Vincent , Floch Romain , Mauguen Maelle , Blanc-Béguin Frédérique , Schick Ulrike , Hamya Mohamed , Abgral Ronan , Le Gal Grégoire , Salaün Pierre-Yves , Lucia François , Le Roux Pierre-Yves
Published: April 2023

Background: Gallium-68 lung perfusion PET/CT is an emerging imaging modality for the assessment of regional lung function, especially to optimise radiotherapy (RT) planning. A key step of lung functional avoidance RT is the delineation of lung functional volumes (LFVs) to be integrated into radiation plans. However, there is currently no consistent and reproducible delineation method for LFVs. The aim of this study was to develop and evaluate an automated delineation threshold method based on total lung function for LFVs delineation with Gallium-68 MAA lung PET/CT imaging. Material and Method: Patients prospectively enrolled in the PEGASUS trial—a pilot study assessing the feasibility of lung functional avoidance using perfusion PET/CT imaging for lung stereotactic body radiotherapy (SBRT) of primary or secondary lesion—were analysed. Patients underwent lung perfusion MAA-68Ga PET/CT imaging and pulmonary function tests (PFTs) as part of pre-treatment evaluation. LFVs were delineated using two methods: the commonly used relative to the maximal pixel value threshold method (pmax threshold method, X%pmax volumes) and a new approach based on a relative to whole lung function threshold method (WLF threshold method, FVX% volumes) using a dedicated iterative algorithm. For both methods, LFVs were expressed in terms of % of the anatomical lung volume (AV) and of % of the total lung activity. Functional volumes were compared for patients with normal PFTs and pre-existing airway disease. Results: 60 patients were analysed. Among the 48 patients who had PFTs, 31 (65%) had pre-existing lung disease. The pmax and WLF threshold methods clearly provided different functional volumes with a wide range of relative lung function for a given pmax volume, and conversely, a wide range of corresponding pmax values for a given WLF volume. The WLF threshold method provided more reliable and consistent volumes with much lower dispersion of LFVs as compared to the pmax method, especially in patients with normal PFTs. Conclusions: We developed a relative to whole lung function threshold segmentation method to delineate lung functional volumes on perfusion PET/CT imaging. The automated algorithm allows for reproducible contouring. This new approach, relatively unaffected by the presence of hot spots, provides reliable and consistent functional volumes, and is clinically meaningful for clinicians.
Functional Alteration in the Brain Due to Tumour Invasion in Paediatric Patients: A Systematic Review
Authors: Sahrizan Nur Shaheera Aidilla , Manan Hanani Abdul , Abdul Hamid Hamzaini , Abdullah Jafri Malin , Yahya Noorazrul
Published: April 2023

Working memory, language and speech abilities, motor skills, and visual abilities are often impaired in children with brain tumours. This is because tumours can invade the brain’s functional areas and cause alterations to the neuronal networks. However, it is unclear what the mechanism of tumour invasion is and how various treatments can cause cognitive impairment. Therefore, this study aims to systematically evaluate the effects of tumour invasion on the cognitive, language, motor, and visual abilities of paediatric patients, as well as discuss the alterations and modifications in neuronal networks and anatomy. The electronic database, PubMed, was used to find relevant studies. The studies were systematically reviewed based on the type and location of brain tumours, cognitive assessment, and pre- and post-operative deficits experienced by patients. Sixteen studies were selected based on the inclusion and exclusion criteria following the guidelines from PRISMA. Most studies agree that tumour invasion in the brain causes cognitive dysfunction and alteration in patients. The effects of a tumour on cognition, language, motor, and visual abilities depend on the type of tumour and its location in the brain. The alteration to the neuronal networks is also dependent on the type and location of the tumour. However, the default mode network (DMN) is the most affected network, regardless of the tumour type and location.Furthermore, our findings suggest that different treatment types can also contribute to patients’ cognitive function to improve or deteriorate. Deficits that persisted or were acquired after surgery could result from surgical manipulation or the progression of the tumour’s growth. Meanwhile, recovery from the deficits indicated that the brain has the ability to recover and reorganise itself.
The Cell Biology of Metastatic Invasion in Pancreatic Cancer: Updates and Mechanistic Insights
Authors: Joshi Vidhu B. , Gutierrez Ruiz Omar L. , Razidlo Gina L.
Published: April 2023

Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes of cancer-related mortality worldwide. This is largely due to the lack of routine screening protocols, an absence of symptoms in early-stage disease leading to late detection, and a paucity of effective treatment options. Critically, the majority of patients either present with metastatic disease or rapidly develop metastatic disease. Thus, there is an urgent need to deepen our understanding of metastasis in PDAC. During metastasis, tumor cells escape from the primary tumor, enter the circulation, and travel to a distant site to form a secondary tumor. In order to accomplish this relatively rare event, tumor cells develop an enhanced ability to detach from the primary tumor, migrate into the surrounding matrix, and invade across the basement membrane. In addition, cancer cells interact with the various cell types and matrix proteins that comprise the tumor microenvironment, with some of these factors working to promote metastasis and others working to suppress it. In PDAC, many of these processes are not well understood. The purpose of this review is to highlight recent advances in the cell biology of the early steps of the metastatic cascade in pancreatic cancer. Specifically, we will examine the regulation of epithelial-to-mesenchymal transition (EMT) in PDAC and its requirement for metastasis, summarize our understanding of how PDAC cells invade and degrade the surrounding matrix, and discuss how migration and adhesion dynamics are regulated in PDAC to optimize cancer cell motility. In addition, the role of the tumor microenvironment in PDAC will also be discussed for each of these invasive processes.
Upregulation of Cell Surface Glycoproteins in Correlation with KSHV LANA in the Kaposi Sarcoma Tumor Microenvironment
Authors: Privatt Sara R. , Ngalamika Owen , Zhang Jianshui , Li Qinsheng , Wood Charles , West John T.
Published: April 2023

HIV-associated epidemic Kaposi sarcoma (EpKS) remains one of the most prevalent cancers in sub-Saharan Africa despite the widespread uptake of anti-retroviral therapy and HIV-1 suppression. In an effort to define potential therapeutic targets against KS tumors, we analyzed previously published KS bulk tumor transcriptomics to identify cell surface biomarkers. In addition to upregulated gene expression (>6-fold) in the EpKS tumor microenvironment, biomarkers were selected for correlation with KSHV latency-associated nuclear antigen (LANA) expression. The cell surface glycoprotein genes identified were KDR, FLT4, ADAM12, UNC5A, ZP2, and OX40, as well as the endothelial lineage determinants Prox-1 and CD34. Each protein was evaluated for its expression and co-localization with KSHV LANA using multi-color immunofluorescence in KS tissues, KSHV-infected L1T2 cells, uninfected TIVE cells, and murine L1T2 tumor xenografts. Five surface glycoproteins (KDR, FLT4, UNC5A, ADAM12, and CD34) were associated with LANA-positive cells but were also detected in uninfected cells in the KS microenvironment. In vitro L1T2 cultures showed evidence of only FLT4, KDR, and UNC5A, whereas mouse L1T2 xenografts recapitulated human KS cell surface expression profiles, with the exception of CD34 and Prox-1. In KS tumors, most LANA-positive cells co-expressed markers of vascular as well as lymphatic endothelial lineages, suggesting KS-associated dedifferentiation to a more mesenchymal/progenitor phenotype.
Technical Aspects of Motor and Language Mapping in Glioma Patients
Authors: Al-Adli Nadeem N. , Young Jacob S. , Sibih Youssef E. , Berger Mitchel S.
Published: April 2023

Gliomas are infiltrative primary brain tumors that often invade functional cortical and subcortical regions, and they mandate individualized brain mapping strategies to avoid postoperative neurological deficits. It is well known that maximal safe resection significantly improves survival, while postoperative deficits minimize the benefits associated with aggressive resections and diminish patients’ quality of life. Although non-invasive imaging tools serve as useful adjuncts, intraoperative stimulation mapping (ISM) is the gold standard for identifying functional cortical and subcortical regions and minimizing morbidity during these challenging resections. Current mapping methods rely on the use of low-frequency and high-frequency stimulation, delivered with monopolar or bipolar probes either directly to the cortical surface or to the subcortical white matter structures. Stimulation effects can be monitored through patient responses during awake mapping procedures and/or with motor-evoked and somatosensory-evoked potentials in patients who are asleep. Depending on the patient’s preoperative status and tumor location and size, neurosurgeons may choose to employ these mapping methods during awake or asleep craniotomies, both of which have their own benefits and challenges. Regardless of which method is used, the goal of intraoperative stimulation is to identify areas of non-functional tissue that can be safely removed to facilitate an approach trajectory to the equator, or center, of the tumor. Recent technological advances have improved ISM’s utility in identifying subcortical structures and minimized the seizure risk associated with cortical stimulation. In this review, we summarize the salient technical aspects of which neurosurgeons should be aware in order to implement intraoperative stimulation mapping effectively and safely during glioma surgery.
Melanoma Clinical Decision Support System: An Artificial Intelligence-Based Tool to Diagnose and Predict Disease Outcome in Early-Stage Melanoma Patients
Authors: Diaz-Ramón Jose Luis , Gardeazabal Jesus , Izu Rosa Maria , Garrote Estibaliz , Rasero Javier , Apraiz Aintzane , Penas Cristina , Seijo Sandra , Lopez-Saratxaga Cristina , De la Peña Pedro Maria , Sanchez-Diaz Ana , Cancho-Galan Goikoane , Velasco Veronica , Sevilla Arrate , Fernandez David , Cuenca Iciar , Cortes Jesus María , Alonso Santos , Asumendi Aintzane , Boyano María Dolores
Published: April 2023

This study set out to assess the performance of an artificial intelligence (AI) algorithm based on clinical data and dermatoscopic imaging for the early diagnosis of melanoma, and its capacity to define the metastatic progression of melanoma through serological and histopathological biomarkers, enabling dermatologists to make more informed decisions about patient management. Integrated analysis of demographic data, images of the skin lesions, and serum and histopathological markers were analyzed in a group of 196 patients with melanoma. The interleukins (ILs) IL-4, IL-6, IL-10, and IL-17A as well as IFNγ (interferon), GM-CSF (granulocyte and macrophage colony-stimulating factor), TGFβ (transforming growth factor), and the protein DCD (dermcidin) were quantified in the serum of melanoma patients at the time of diagnosis, and the expression of the RKIP, PIRIN, BCL2, BCL3, MITF, and ANXA5 proteins was detected by immunohistochemistry (IHC) in melanoma biopsies. An AI algorithm was used to improve the early diagnosis of melanoma and to predict the risk of metastasis and of disease-free survival. Two models were obtained to predict metastasis (including “all patients” or only patients “at early stages of melanoma”), and a series of attributes were seen to predict the progression of metastasis: Breslow thickness, infiltrating BCL-2 expressing lymphocytes, and IL-4 and IL-6 serum levels. Importantly, a decrease in serum GM-CSF seems to be a marker of poor prognosis in patients with early-stage melanomas.
Disequilibrium between BRCA1 and BRCA2 Circular and Messenger RNAs Plays a Role in Breast Cancer
Authors: Levacher Corentin , Viennot Mathieu , Drouet Aurélie , Beaussire Ludivine , Coutant Sophie , Théry Jean-Christophe , Baert-Desurmont Stéphanie , Laé Marick , Ruminy Philippe , Houdayer Claude
Published: April 2023

Breast cancer is a frequent disease for which the discovery of markers that enable early detection or prognostic assessment remains challenging. Circular RNAs (circRNAs) are single-stranded structures in closed loops that are produced by backsplicing. CircRNA and messenger RNA (mRNA) are generated co-transcriptionally, and backsplicing and linear splicing compete against each other. As mRNAs are key players in tumorigenesis, we hypothesize that a disruption of the balance between circRNAs and mRNAs could promote breast cancer. Hence, we developed an assay for a simultaneous study of circRNAs and mRNAs, which we have called splice and expression analyses by exon ligation and high-throughput sequencing (SEALigHTS). Following SEALigHTS validation for BRCA1 and BRCA2, our hypothesis was tested using an independent research set of 95 pairs from tumor and adjacent normal breast tissues. In this research set, ratios of BRCA1 and BRCA2 circRNAs/mRNAs were significantly lower in the tumor breast tissue compared to normal tissue (p = 1.6 × 10−9 and p = 4.4 × 10−5 for BRCA1 and BRCA2, respectively). Overall, we developed an innovative method to study linear splicing and backsplicing, described the repertoire of BRCA1 and BRCA2 circRNAs, including 15 novel ones, and showed for the first time that a disequilibrium between BRCA1 and BRCA2 circRNAs and mRNAs plays a role in breast cancer.
Staged or Simultaneous Surgery for Colon or Rectal Cancer with Synchronous Liver Metastases: Implications for Study Design and Clinical Endpoints
Authors: Yaqub Sheraz , Margonis Georgios Antonios , Søreide Kjetil
Published: April 2023

In patients presenting with colorectal cancer and synchronous liver metastases, the disease burden related to the liver metastasis is the driving cause of limited longevity and, eventually, risk of death. Surgical resection is the potentially curative treatment for colorectal cancer liver metastases. In the synchronous setting where both the liver metastases and the primary tumor are resectable with a relative low risk, the oncological surgeon and the patient may consider three potential treatment strategies. Firstly, a “staged” or a “simultaneous” surgical approach. Secondly, for a staged strategy, a ‘conventional approach’ will suggest removal of the primary tumor first (either colon or rectal cancer) and plan for liver surgery after recovery from the first operation. A “Liver first” strategy is prioritizing the liver resection before resection of the primary tumor. Planning a surgical trial investigating a two-organ oncological resection with highly variable extent and complexity of resection as well as the potential impact of perioperative chemo(radio)therapy makes it difficult to find the optimal primary endpoint. Here, we suggest running investigational trials with carefully chosen composite endpoints as well as embedded risk-stratification strategies to identify subgroups of patients who may benefit from simultaneous surgery.
Tumor-Infiltrating B Lymphocytes: Promising Immunotherapeutic Targets for Primary Liver Cancer Treatment
Authors: Milardi Giulia , Lleo Ana
Published: April 2023

Hepatocellular carcinoma and cholangiocarcinoma are the fourth most lethal primary cancers worldwide. Therefore, there is an urgent need for therapeutic strategies, including immune cell targeting therapies. The heterogeneity of liver cancer is partially explained by the characteristics of the tumor microenvironment (TME), where adaptive and innate immune system cells are the main components. Pioneering studies of primary liver cancers revealed that tumor-infiltrating immune cells and their dynamic interaction with cancer cells significantly impacted carcinogenesis, playing an important role in cancer immune evasion and responses to immunotherapy treatment. In particular, B cells may play a prominent role and have a controversial function in the TME. In this work, we highlight the effect of B lymphocytes as tumor infiltrates in relation to primary liver cancers and their potential prognostic value. We also present the key pathways underlying B-cell interactions within the TME, as well as the way that a comprehensive characterization of B-cell biology can be exploited to develop novel immune-based therapeutic approaches.
The Prognostic Value of Neutrophil-to-Lymphocyte Ratio and Platelet-to-Lymphocyte Ratio for Small Renal Cell Carcinomas after Image-Guided Cryoablation or Radio-Frequency Ablation
Authors: Asif Aqua , Chan Vinson Wai-Shun , Osman Filzah Hanis , Koe Jasmine Sze-Ern , Ng Alexander , Burton Oliver Edward , Cartledge Jon , Kimuli Michael , Vasudev Naveen , Ralph Christy , Jagdev Satinder , Bhattarai Selina , Smith Jonathan , Lenton James , Wah Tze Min
Published: April 2023

There is a lack of cheap and effective biomarkers for the prediction of renal cancer outcomes post-image-guided ablation. This is a retrospective study of patients with localised small renal cell cancer (T1a or T1b) undergoing cryoablation or radiofrequency ablation (RFA) at our institution from 2003 to 2016. A total of 203 patients were included in the analysis. In the multivariable analysis, patients with raised neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) pre-operatively, post-operatively and peri-operatively are associated with significantly worsened cancer-specific survival, overall survival and metastasis-free survival. Furthermore, an increased PLR pre-operatively is also associated with increased odds of a larger than 25% drop in renal function post-operatively. In conclusion, NLR and PLR are effective prognostic factors in predicting oncological outcomes and peri-operative outcomes; however, larger external datasets should be used to validate the findings prior to clinical application.
Asymmetric Synthesis of trans-3-Alkoxyamino-4-Oxygenated-2-Piperidones Mediated by Transition-Metal-Free Dual C-H Oxidation and Its CAL-B-Assisted Enzymatic Resolution
Authors: Romero-Ibañez Julio , Ortega-Rojas Marina A. , Valdéz-Camacho Jonathan R. , Hernández-Vázquez Luis G. , Sartillo-Piscil Fernando , Escalante Jaime
Published: April 2023

A general chemo-enzymatic approach to synthesize both enantioenriched trans-3-alkoxyamino-4-oxy-2-piperidones, which are important scaffold for various naturally occurring alkaloids, is reported. To this end, a selective transition-metal-free dual C−H oxidation of piperidines mediated by the TEMPO oxoammonium cation (TEMPO+) was used, followed by enzymatic resolution of the corresponding alkoxyamino-2-piperidones with Candida antarctica lipase (CAL-B), to yield the title compounds in high enantiomeric excess (ee). The absolute configuration of both enantioenriched compounds was determined using chemical correlation and circular dichroism (CD) spectroscopy. The former method highlights the oxidative ring contraction of the trans-alkoxyamine-2-piperidone ring into its corresponding 2-pyrrolidinone.
In Silico Structural and Functional Analysis of the Mitochondrial Malate Transporters in Oleaginous Fungus Mucor circinelloides WJ11
Authors: Yang Wu , Mohamed Hassan , Shah Aabid Manzoor , Zhang Huaiyuan , Pang Shuxian , Shi Wenyue , Xue Futing , Song Yuanda
Published: April 2023

Malate transporter proteins (MTPs) play a pivotal role in regulating flux in the citrate/malate/pyruvate shuttle to deliver acetyl-CoA from the mitochondria to the cytosol and thus regulate lipid biosynthesis in oleaginous fungi. Despite the recent successful exploration of the mitochondrial malate transporters in Mucor circinelloides, research with in silico analyses that include molecular docking and their dynamics, in addition to homology modelling of malate transporters, have not been reported. In this study, the physico-chemical properties and nucleotide sequence analysis of two mitochondrial MTPs (MT and SoDIT-a with Gene/protein ID scafold00018.48 and scafold00239.15, respectively), in M. circinelloides WJ11 were performed. The three-dimensional (3D) model of the mitochondrial MTPs was determined and the best-docked complex stabilities were demonstrated with molecular dynamic (MD) simulations. The activity domain was revealed to form hydrogen bonds and piling interactions with citrate and malate upon docking. Our study showed better binding affinities for the MTPs—reaching up to −3.44 and −7.27 kcal/mol with the MT and SoDIT-a proteins, respectively (compared to the target of −2.85 and −6.00 kcal/mol for citric acid-binding). MD simulations illustrated that the protein complexes demonstrated conformational stability throughout the simulation. This study was the first to elucidate the structural characteristics of mitochondrial MTPs in M. circinelloides WJ11, providing direct evidence regarding the transport mechanism of specific substrates. Furthermore, the current results support ongoing efforts to combine functional and structural data to better understand the MTPs (at the molecular and atomic levels) of an oleaginous fungus such as M. circinelloides.
Early Alterations of RNA Binding Protein (RBP) Homeostasis and ER Stress-Mediated Autophagy Contributes to Progressive Retinal Degeneration in the rd10 Mouse Model of Retinitis Pigmentosa (RP)
Authors: Yamoah Alfred , Tripathi Priyanka , Guo Haihong , Scheve Leonie , Walter Peter , Johnen Sandra , Müller Frank , Weis Joachim , Goswami Anand
Published: April 2023

The retinal degeneration 10 (rd10) mouse model is widely used to study retinitis pigmentosa (RP) pathomechanisms. It offers a rather unique opportunity to study trans-neuronal degeneration because the cell populations in question are separated anatomically and the mutated Pde6b gene is selectively expressed in rod photoreceptors. We hypothesized that RNA binding protein (RBP) aggregation and abnormal autophagy might serve as early pathogenic events, damaging non-photoreceptor retinal cell types that are not primarily targeted by the Pde6b gene defect. We used a combination of immunohistochemistry (DAB, immunofluorescence), electron microscopy (EM), subcellular fractionation, and Western blot analysis on the retinal preparations obtained from both rd10 and wild-type mice. We found early, robust increases in levels of the protective endoplasmic reticulum (ER) calcium (Ca2+) buffering chaperone Sigma receptor 1 (SigR1) together with other ER-Ca2+ buffering proteins in both photoreceptors and non-photoreceptor neuronal cells before any noticeable photoreceptor degeneration. In line with this, we found markedly altered expression of the autophagy proteins p62 and LC3, together with abnormal ER widening and large autophagic vacuoles as detected by EM. Interestingly, these changes were accompanied by early, prominent cytoplasmic and nuclear aggregation of the key RBPs including pTDP-43 and FET family RBPs and stress granule formation. We conclude that progressive neurodegeneration in the rd10 mouse retina is associated with early disturbances of proteostasis and autophagy, along with abnormal cytoplasmic RBP aggregation.
Correction: Peng et al. Distinct Dominant Lineage from In Vitro Expanded Adipose-Derived Stem Cells (ASCs) Exhibits Enhanced Wound Healing Properties. Cells 2022, 11, 1236
Authors: Peng Qiuyue , Ren Guoqiang , Xuan Zongzhe , Duda Martyna , Pennisi Cristian Pablo , Porsborg Simone Riis , Fink Trine , Zachar Vladimir
Published: April 2023

In the original publication [...]
Effective Adsorptive Removal of Coomassie Violet Dye from Aqueous Solutions Using Green Synthesized Zinc Hydroxide Nanoparticles Prepared from Calotropis gigantea Leaf Extract
Authors: Parimelazhagan Vairavel , Natarajan Kannan , Shanbhag Srinath , Madivada Sumanth , Kumar Harish S.
Published: April 2023

The removal of color from dye wastewater is crucial, since dyes are extremely toxic and can cause cancer in a variety of life forms. Studies must be done to use cost-effective adsorbents for the removal of color from dye effluents to protect the environment. To our knowledge, virtually no research has been done to describe the possibility of using Calotropis gigantea leaf extract zinc hydroxide nanoparticles (CG-Zn(OH)2NPs) as an adsorbent for the decolorization of Coomassie violet (CV) from the aqueous emulsion, either in batch mode or continuously. In the present batch investigation, CV dye is removed from the synthetic aqueous phase using CG-Zn(OH)2NPs as an adsorbent. The synthesized nanoparticles were characterized using various instrumental techniques such as Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDS) and Brunauer–Emmett–Teller (BET) surface area and pore volume, a particle size analyser, and zero-point charge. The decolorization efficacy of CV dye from an aqueous phase by the adsorbent was examined in batch mode by varying process parameters. The consequences of various experimental variables were optimized using response surface methodology (RSM) to achieve the maximum decolorization efficiency (90.74%) and equilibrium dye uptake, qe (35.12 mg g−1). The optimum pH, dye concentration, CG-Zn(OH)2NPs adsorbent dosage, and particle size were found to be 1.8, 225 mg L−1, 5 g L−1, and 78 μm, respectively for CV dye adsorption capacity at equilibrium. The adsorbent zero-point charge was found to be at pH 8.5. The Langmuir isotherm model provided a good representation of the equilibrium data in aqueous solutions, with a maximum monolayer adsorption capability (qmax) of 40.25 mg g−1 at 299 K. The dye adsorption rate follows a pseudo-second-order kinetic model at various dye concentrations, which indicated that the reaction is more chemisorption than physisorption. The negative values of ΔG and positive values of ΔH at different temperatures indicate that the adsorption process is spontaneous and endothermic, respectively. Reusability tests revealed that the prepared nanoparticles may be used for up to three runs, indicating that the novel CG-Zn(OH)2NPs seems to be a very promising adsorbent for the removal of Coomassie violet dye from wastewater.
Production of Alkyl Levulinates from Carbohydrate-Derived Chemical Intermediates Using Phosphotungstic Acid Supported on Humin-Derived Activated Carbon (PTA/HAC) as a Recyclable Heterogeneous Acid Catalyst
Authors: Vinod Nivedha , Dutta Saikat
Published: April 2023

This work reports a straightforward and high-yielding synthesis of alkyl levulinates (ALs), a class of promising biofuel, renewable solvent, and chemical feedstock of renewable origin. ALs were prepared by the acid-catalyzed esterification of levulinic acid (LA) and by the alcoholysis of carbohydrate-derived chemical platforms, such as furfuryl alcohol (FAL) and α-angelica lactone (α-AGL). Phosphotungstic acid (PTA) was chosen as the solid acid catalyst for the transformation, which was heterogenized on humin-derived activated carbon (HAC) for superior recyclability. Using HAC as catalyst support expands the scope of valorizing humin, a complex furanic resin produced inevitably as a side product (often considered waste) during the acid-catalyzed hydrolysis/dehydration of sugars and polymeric carbohydrates. Under optimized conditions (150 °C, 7 h, 25 wt.% of 20%PTA/HAC-600 catalyst), ethyl levulinate (EL) was obtained in an 85% isolated yield starting from FAL. Using the general synthetic protocol, EL was isolated in 88% and 84% yields from LA and α-AGL, respectively. The 20%PTA/HAC-600 catalyst was successfully recovered from the reaction mixture and recycled for five cycles. A marginal loss in the yield of ALs was observed in consecutive catalytic cycles due to partial leaching of PTA from the HAC support.
Fluorescent Sensors for Detecting and Imaging Metal Ions in Biological Systems: Recent Advances and Future Perspectives
Authors: Shi Yang , Zhang Wenxian , Xue Yi , Zhang Jingjing
Published: April 2023

Metal ions play a crucial role in many biochemical processes, and when in a state of scarcity or surplus, they can lead to various diseases. Therefore, the development of a selective, sensitive, cost-effective, and fast-responding sensor to detect metal ions is critical for in vitro medical diagnostics. In recent years, fluorescent sensors have been extensively investigated as potent kits for the effective assessment of metal ions in living systems due to their high sensitivity, selectivity, ability to perform real-time, non-invasive monitoring, and versatility. This review is an overview of recent advances in fluorescent sensors for the detection and imaging of metal ions in biosystems from 2018 to date. Specifically, we discuss their application in detecting essential metal ions and non-essential metal ions for in vitro diagnostics, living cell imaging, and in vivo imaging. Finally, we summarize remaining challenges and offer a future outlook on the above topics.
Ureterocystoplasty in Boys with Valve Bladder Syndrome—Is This Method Still up to Date?
Authors: Hofmann Aybike , Ioannou Alexandros , Zöhrer Pirmin Irenaeus , Rösch Wolfgang H.
Published: April 2023

Boys with valve bladder syndrome (PUV) require adequate treatment of the lower urinary tract to preserve renal function and improve long-term outcomes. In some patients, further surgery may be necessary to improve bladder capacity and function. Ureterocytoplasty (UCP) is usually carried out with a small segment of intestine or, alternatively, with a dilated ureter. Our aim was to evaluate the long-term outcomes after UCP in boys with PUV. UCP had been performed in 10 boys with PUV at our hospital (2004–2019). Pre- and postoperative data were evaluated in relation to kidney and bladder function, the SWRD score, additional surgery, complications, and long-term follow-up. The mean time between primary valve ablation and UCP was 3.5 years (SD ± 2.0). The median follow-up time was 64.5 months (IQR 36.0–97.25). The mean increase in age-adjusted bladder capacity was 25% (from 77% (SD ± 0.28) to 102% (SD ± 0.46)). Eight boys micturated spontaneously. Ultrasounds showed no severe hydronephrosis (grade 3–4). The SWRD score showed a median decrease from 4.5 (range 2–7) to 3.0 (range 1–5). No conversion of augmentation was required. UCP is a safe and effective approach to improve bladder capacity in boys with PUV. In addition, the possibility of micturating naturally is still maintained.
Attitudes toward the Care of Children with Cancer in Saudi: An Exploratory Survey
Authors: Alahmad Ghiath
Published: April 2023

The ethical challenges of pediatric cancer care across Arab countries are not well addressed, despite medical advancements and increased awareness of children’s rights. The ethical challenges related to pediatric cancer in Saudi Arabia were investigated by surveying 400 respondents at King Abdulaziz Medical City in Riyadh, Jeddah, and Dammam, Saudi Arabia, from four groups: pediatricians, medical students, nurses, and parents of children with cancer. Respondents’ characteristics were surveyed across three outcomes: awareness of care, knowledge, and parent consent/child assent, developed from a systematic review and a qualitative analysis. A majority of respondents (89.0%) considered pediatric cancer different from adult cancer. Families considered alternative treatment, according to 64.3% of respondents, while 88.0% emphasized understanding the family’s needs and values. Furthermore, 95.8% of respondents believed physicians should offer time for pedagogy, 92.3% viewed parental consent as essential, and 94.5% thought that sufficient discussion about the plan and type of treatment should precede consent. However, child assent showed lower levels of agreement, with only 41.3% and 52.5% agreeing with getting child assent and having a discussion. Finally, 56% agreed that parents might refuse suggested treatment, while only 24.3% agreed that the child could refuse it. In all these ethical considerations, nurses and physicians showed significantly more positive results compared with other groups.
Identification of MicroRNAs Regulating Clostridium perfringens Type C Infection in the Spleen of Diarrheic Piglets
Authors: Wang Pengfei , Yang Qiaoli , Yan Zunqiang , Huang Xiaoyu , Gao Xiaoli , Gun Shuangbao
Published: April 2023

Clostridium perfringens (C. perfringens) type C is one of the common bacteria in piglet diarrhea, which seriously affects the swine industry’s development. The spleen plays crucial roles in the resistance and elimination of pathogenic microorganisms, and miRNAs play important roles in regulating piglet diarrhea caused by pathogens. However, the mechanism by which miRNAs in the spleen are involved in regulating C. perfringens type C causing diarrhea in piglets remains unclear. The expression profiles of the spleen miRNAs of 7-day-old piglets challenged by C. perfringens type C were studied using small RNA-sequencing in control (SC), susceptible (SS), and resistant (SR) groups. Eight-eight differentially expressed miRNAs were screened. The KEGG pathway analysis of target genes revealed that the miRNAs were involved in the MAPK, p53, and ECM–receptor interaction signaling pathways. NFATC4 was determined to be a direct target of miR-532-3p and miR-133b using a dual-luciferase reporter assay. Thus, miR-133b and miR-532-3p targeted to NFATC4 were likely involved to piglet resistance to C. perfringens type C. This paper provides the valuable resources to deeply understand the genetic basis of C. perfringens type C resistance in piglets and a solid foundation to identify novel markers of C. perfringens type C resistance.
Viticultural Manipulation and New Technologies to Address Environmental Challenges Caused by Climate Change
Authors: Sun Qun , Granco Gabriel , Groves Leah , Voong Jully , Van Zyl Sonet
Published: April 2023

Climate change is a critical challenge for the global grape and wine industry, as it can disrupt grapevine growth, production, and wine quality. Climate change could influence the cost-effectiveness and growth of the wine industry in different wine regions since grapevine development is deeply dependent on weather (short-term) and climate (long-term) conditions. Innovation and new technologies are needed to meet the challenge. This review article addresses the impact of climate change on grapevines, such as vine phenology, pest and disease pressure, crop load, and grape and wine composition. It also reviews recent advances in the areas of viticultural manipulation and relevant technologies to potentially reduce the impact of climate change and help growers improve grape quality. Remote sensing is used for vineyard microclimate monitoring; thermal sensors combined with UAVs, aircraft, or satellites are used for water management; soil electrical conductivity sensors have been developed for soil mapping. Viticultural manipulations, such as regulated deficit irrigation for water use efficiency and berry-ripening delay for growing quality fruit, are also discussed. The review assesses future directions for further technological development, such as soil and vine water monitoring devises, precision viticulture, and artificial intelligence in vineyards.
Temporal Changes in Minimum and Maximum Temperatures at Selected Locations of Southern Africa
Authors: Mupangwa Walter , Chipindu Lovemore , Ncube Bongani , Mkuhlani Siyabusa , Nhantumbo Nascimento , Masvaya Esther , Ngwira Amos , Moeletsi Mokhele , Nyagumbo Isaiah , Liben Feyera
Published: April 2023

Agriculture is threatened by ever increasing temperatures and this trend is predicted to continue for the near and distant future. The negative impact of rising temperatures on agri-food systems is also compounded by the erratic and highly variable rainfall in most parts of southern Africa. Minimum and maximum temperatures’ variability and trend analysis were undertaken using daily time series data derived from 23 meteorological stations spread across Malawi, Mozambique, South Africa and Zimbabwe. The modified Mann–Kendall and Theil–Sen slope models were used to assess temperature trends and their magnitudes. Temperature varied with location and minimum temperature was more variable than maximum temperature. Semi-arid regions had higher variation in minimum temperature compared to humid and coastal environments. The results showed an upward trend in minimum (0.01–0.83 °C over a 33–38 year period) and maximum (0.01–0.09 °C over a 38–57 year period) temperatures at 9 and15 locations, respectively. A downward trend in minimum temperature (0.03–0.20 °C over 38–41 years) occurred in South Africa at two locations and Dedza (Malawi), while a non-significant decline in maximum temperature (0.01 °C over 54 years) occurred at one location in coastal dry sub-humid Mozambique. The results confirm the increase in temperature over 33–79 years, and highlight the importance of including temperature when designing climate change adaption and mitigation strategies in southern Africa and similar environments.
Traumatic Brain Injury, Sleep, and Melatonin—Intrinsic Changes with Therapeutic Potential
Authors: Bell Allen , Hewins Bryson , Bishop Courtney , Fortin Amanda , Wang Jonathan , Creamer Jennifer L. , Collen Jacob , Werner J. Kent
Published: April 2023

Traumatic brain injury (TBI) is one of the most prevalent causes of morbidity in the United States and is associated with numerous chronic sequelae long after the point of injury. One of the most common long-term complaints in patients with TBI is sleep dysfunction. It is reported that alterations in melatonin follow TBI and may be linked with various sleep and circadian disorders directly (via cellular signaling) or indirectly (via free radicals and inflammatory signaling). Work over the past two decades has contributed to our understanding of the role of melatonin as a sleep regulator and neuroprotective anti-inflammatory agent. Although there is increasing interest in the treatment of insomnia following TBI, a lack of standardization and rigor in melatonin research has left behind a trail of non-generalizable data and ambiguous treatment recommendations. This narrative review describes the underlying biochemical properties of melatonin as they are relevant to TBI. We also discuss potential benefits and a path forward regarding the therapeutic management of TBI with melatonin treatment, including its role as a neuroprotectant, a somnogen, and a modulator of the circadian rhythm.
Thin Films and Coatings for Energy Storage and Conversion: From Supercapacitors and Batteries to Hydrogen Generators
Authors: Ondrejka Peter , Mikolášek Miroslav
Published: April 2023

The transition to a green economy is becoming an important challenge for sustainable economic growth [...]
Investigations into Power Plant Alloys’ (Inconel 718) Oxidation Resistance by Compound Composite (Cr2O3 + YSZ) Coatings
Authors: Yunus Mohammed , Alfattani Rami A. , Alamro Turki , Asadullah Mohammed
Published: April 2023

As it increases the pressure and temperature of incoming steam and decreases CO2 emissions, oxidation is crucial for materials used in power plants to increase their efficiency. Compound composite (Cr2O3 + YSZ) coatings applied to Inconel 718 (EN8) substrates using the Atmosphere Plasma Spray technique are anticipated to increase structural resistance when subjected to high pressure and temperature oxidation conditions of service. The nickel-based superalloys EN8 and EN8/Yttria (8% Y2O3) Stabilized Zirconia (YSZ)/Cr2O3 were subjected to high-temperature oxidation tests in the open air at 1050 °C for approximately 12, 24, 48, and 100 h. EN8 is not appropriate for prolonged use at 1050 °C as can be seen from scanned electron microscope and energy dispersive X-ray spectroscopy analyses on isothermally oxidized samples. The findings demonstrated that the EN8 alloy exhibited more significant weight variations over 48 h at high temperatures because its chromia oxide scale was continuously smaller. With phase dispersion in the microstructure, coated EN8 exhibits a higher performance under more prolonged exposure than the EN8 alloy. Additionally, the synthesis of outer chromium oxide, YSZ, and Cr2O3 on a substrate at 1050 °C for 100 h improved the outstanding oxidation resistance while maintaining the integrity of the chromium oxide layer.
Chlorogenic Acid, a Component of Oenanthe javanica (Blume) DC., Attenuates Oxidative Damage and Prostaglandin E2 Production Due to Particulate Matter 10 in HaCaT Keratinocytes
Authors: Bae In Ah , Ha Jae Won , Boo Yong Chool
Published: April 2023

Oenanthe javanica (OJ) is a perennial herb that grows wildly or is cultivated in Asia, and it is used as food or in traditional medicine. The antioxidant and anti-inflammatory effects of OJ-derived materials have been extensively explored previously, but their effects on the cytotoxicity of air pollution are currently unknown. Therefore, the present study aimed to evaluate the effect of the hot water extract of OJ on atmospheric particulate matter 10 (PM10)-induced cytotoxicity and oxidative damage in human HaCaT keratinocytes, and to identify its active ingredient and mechanism of action. When the hot water extract of OJ was divided into methylene chloride, ethyl acetate (EA), n-butanol (BA), and water fractions, caffeic acid was enriched in the EA fraction and chlorogenic acid was enriched in the BA fraction. PM10 increased reactive oxygen species (ROS) production, lipid peroxidation, protein carbonylation, and inflammatory prostaglandin (PG) E2 production in cells. The BA fraction reduced the PM10-induced ROS production in cells more effectively than the total extract and other solvent fractions. Chlorogenic acid was more effective in reducing ROS levels than caffeic acid and N-acetyl cysteine (NAC). Chlorogenic acid attenuated the increase in lipid peroxidation and the PG E2 production of cells due to PM10 exposure. Of the genes involved in PG E2 production, phospholipase A2 group IVA (PLA2G4A), Prostaglandin-endoperoxide synthase 1 (PTGS1), and 2 (PTGS2) were transcriptionally up-regulated by PM10, whereas phospholipase A2 group IIA (PLA2G2A) was down-regulated and prostaglandin E synthetase 1 (PTGES1) and 2 (PTGES2) were a little altered. The PM10-induced increase in PLA2G4A mRNA was alleviated by chlorogenic acid and NAC. Accordingly, PM10 increased the expression levels of cytosolic phospholipase A2 (cPLA2) protein and its phosphorylated form, which were attenuated by chlorogenic acid and NAC. Thus, chlorogenic acid may attenuate the PM10-induced PG E2 production through the suppression of PLA2G4A mRNA and cPLA2 protein expressions. This study suggests that chlorogenic acid contained in OJ extract may help alleviate the oxidative damage to and inflammatory responses of the skin cells due to exposure to air pollutants.
On the Hot Deformation of a Fe-Al-Ta Iron Aluminide Prepared via Laser Powder Bed Fusion
Authors: Emdadi Aliakbar , Bolz Sebastian , Jensch Felix , Tovar Michael , Weiß Sabine
Published: April 2023

In the present work, a combined process of laser powder bed fusion (LPBF) and hot working in terms of microstructure refinement was investigated for Fe-25Al-1.5Ta alloy samples. Uniaxial compression tests were carried out parallel and perpendicular to the building direction (BD) at 1000 °C, where BCC A2-phase was stable, at a strain rate of 0.0013 s−1. The true stress–true strain curves indicated a broad flow stress peak followed by a slight decrease, which is typical for dynamic recrystallization (DRX) of conventional BCC metals such as ferritic iron. A negligible dependence in the flow stress behavior on the compression direction was observed. DRX initiated at a stress of 18.7 MPa for the sample compressed parallel to the BD, corresponding to a true strain of 0.011, and at 18.1 MPa for the samples compressed normal to the BD, which corresponded to a true strain of 0.010. The microstructural investigations by electron backscatter diffraction (EBSD) showed that the relatively coarse and elongated grains of the as-LPBF builds were significantly refined after hot working. The microstructure of the compressed samples mainly consisted deformed grains. These were fragmented by sub-grains bounded by low-angle boundaries independent of the compression axis, indicating the occurrence of dynamic recovery (DRV) during hot working. In addition, a few equiaxed, small grains were observed in the pre-existing grain boundaries, which formed due to DRX. Most pores in the as-LPBF builds were closed after hot compression, particularly in the central region of the deformed specimens where the compressive stress state is dominant. In summary, hot compression reveals a practical thermomechanical post-processing treatment for Fe-Al-Ta iron aluminides built by LPBF. The hot working refines the epitaxially elongated microstructure of the as-LPBF builds by DRV/DRX and reduces the porosity.
Multicolour Laser Emission Based on the Polystyrene Dust
Authors: Szukalska Alina , Mysliwiec Jaroslaw , Szukalski Adam
Published: April 2023

Multicolour emission, independently of the considered mechanism, constitutes an appealing application for modern technologies and an interesting phenomenon for the various fields of science. Moreover, effective and easy colour tuning seems even more engaging. Through this contribution, we present a totally new approach to the organic-based system, which can provide effective and multicolour emission, including laser action. Polystyrene dust (PSd) particles play the role of the optically passive matrix for highly effective laser dyes (Coumarin 504, Coumarin 540, and DCM), which were successfully introduced into the polymer units by liquid crystals carrier. In such a way, various types of organic hybrid systems were created, starting from a single dye embedded in PSd volume, passing by two dye-component architectures, reaching the final and most complex network containing three chromophores, providing three basic tones (red, green, and blue). Moreover, by playing with an optical pumping magnitude, we were able to easily modulate colour tones/shades in a broad range, which is highly appreciated when considering human eye perception.
Modelling and Optimization of Continual Laser Joining Processes for Silicon Aluminum Alloy in Microwave Devices
Authors: Wang Song , Shi Ge , Zhao Libo , Dai Yanwei , Hou Tianyu , He Ying , Chen Ping , Qin Fei
Published: April 2023

Due to its higher energy and smaller heating area, laser joining technology is widely used in aluminum alloy welding and other industrial fields, which meets the solder sealing requirements for electronic packaging. According to experiments, cracks were prone to occur at the corners and spot-welding positions near the weld. In this paper, the depth and width of the melt pool were measured experimentally, and the results were used to calibrate and validate the heat source model. An empirical relationship between heat source parameters and melt pool morphology is presented. The heat source model of laser deep penetration welding was established under the same experimental conditions. And the results were in agreement with the experimental results. The finite element method was used to numerically simulate the welding process of a 50%SiAl shell and a 27%SiAl cover plate. The effects of different spot-welding sequences and numbers on the residual stress and cracking possibility of laser welded samples were analyzed. The results show that under sequential spot-welding, when the amount of spot-welding is increased, the stress peak value decreases. Compared with sequential spot welding and side-by-side spot welding, the spot-welding sequence of diagonal points first, and then side-by-side spot welding, can effectively reduce the residual stress. This research enables us to provide some guidelines in terms of studying the reliability issues of microwave devices.