bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Ticagrerol versus Clopidogrel on Inflammatory Bio-marker in Patients with Chronic Stable Angina Patients after Percutaneous Coronary Intervention
Authors: Sharmin Elora , Dewan Jasmine Fauzia , Ahasan Syed Ali , Ahmed Sheikh Foyez , Haque Pritha Puloma , Zaman SM Mustafa , Quader Rezwaunl , Hoque Harisul
Published: May 2016

Background: Ischemic heart disease remains the leading cause of death in both developed and under developed countries. The use of antiplatelet drugs specifically the thienopyridine has become a standard for the treatment of acute coronary syndrome. These drugs irreversibly inhibit the platelet aggregation by blocking the P2Y12 receptor. But currently this therapeutic choice has become limited due to potential interaction with other drugs, slow hepatic conversion, genetic resistance and narrow therapeutic safety margin. Ticagrerol, a reversible P2Y12 receptor inhibitor may represent a significant advancement over currently available oral antiplatelet drugs.Objectives: The study was intended to compare the effect of Ticagrelor and Clopidogrel on inflammatory and oxidative stress markers in patients of chronic stable angina (CSA) following percutaneous coronary intervention (PCI).Materials & Methods: The present prospective observational study was carried out in the Department of Pharmacology, Cardiology and Microbiology, BSMMU, Dhaka from September 2014 to February 2016. The study included a total of 100 CSA patients. Patients were divided into two groups, Ticagrelor and Clopidogrel treated groups (each having 50 patients). The baseline laboratory parameters- High sensitive C-reactive protein (hs-CRP), bleeding time, clotting time, were measured and then patients of both groups underwent PCI. The same parameters were again assessed at follow up after 4 weeks of intervention. Total 12 patients from Ticagrelor and 14 patients from Clopidogrel groups were dropped out. Comparisons of the laboratory parameters were made between two groups at baseline and at follow up and also within group before and after intervention. Result: In the present study at baseline characteristics of patients treated with Ticagrelor and Clopidogrel were almost identical in terms of age, sex, diabetes and hypertension. The inflammatory marker hs-CRP was also similar in both groups at baseline. At follow up hs-CRP was significantly reduced from baseline 19.7 mg/dl to 1.7 mg/dl (p value- 0.001) in ticagrerol group and 18.4 mg/dl to 2 mg/dl (p value-0.001) in clopidogrel group. There was no significant change in bleeding time and clotting time in both groups of patients.Conclusion: The study concluded that both Ticagrelor and Clopidogrel are similar in improving the status of the inflammatory marker, resulting from inflammatory processes in patients of chronic stable angina.University Heart Journal Vol. 11, No. 1, January 2015; 42-44
DerridaFloruit
Authors: Naas Michael
Published: May 2016

The word floruit is typically used to designate the year around which a thinker or writer is thought to have ‘flourished’. Traditionally, that age is set at forty. In this paper, I ask whether texts too might be assigned a time of flourishing, a floruit – or perhaps more than one – that would no longer be attached to the life of the author but to the unique time of the trace or the archive, a flourishing that might best be thought in terms of what Derrida called survivance or living-on. I develop this notion of a flourishing beyond the life of the author, indeed beyond the human altogether, by returning to Glas, a text that was published forty years ago and that is soon to undergo – or at least this is my hope – a new infusion of critical interest and a new flourishing. I argue that by returning to Glas and its seminal analyses of Hegel and Genet on the themes of life, death, blood, spirit, glory, the death penalty, the proper of man, and so on, we will be better able to understand and take the measure of Derrida's return to these same themes in his final seminars a quarter of a century later.
The Play of the World: The End, the Great Outdoors, the Outside, Alterity and the Real
Authors: Colebrook Claire
Published: May 2016

Both in his earliest debates with thinkers such as Foucault and Levinas, and in later critiques of political immediacy, Derrida invoked the inescapable burden of a necessary but impossible universalism. By raising the stakes so high it would seem that deconstruction generates hyperbolic conceptions of ethics and justice, but also precludes any form of day to day political positivity. In this essay I pursue the seemingly less ‘ethical’ conception of play in Derrida's work to argue for a multiple universalism.
Grammatology: A Vital Science
Authors: Kirby Vicki
Published: May 2016

This essay argues that Jacques Derrida's early work on grammatology and the science of writing continues to have interventionary relevance for how we understand the scientific enterprise today. Catherine Malabou has argued that the importance of Derrida's contribution has waned because the metaphors and tropes that he deploys have been eclipsed with time, becoming less applicable in explanations of how things work. Offering her own replacement term, ‘plastology’, Malabou argues that insights into the operations of brain plasticity, for example, have superseded graphic metaphors. I suggest that Malabou's remedial is misguided because Derrida's ‘graphematic structure’ was never intended to answer the problem of representational accuracy. Indeed, Derrida advises that his non-concepts ‘trace’, ‘gramme’ or ‘writing’ are no more graphic than phonological, no more temporal than spatial. I argue, with Derrida, that a model, or representation, is not a third term in-between the biologist and biology or the writer and the world. In grammatology's onto-epistemological confounding of subject with/in object, we find that ‘writing's’ riddles also appear in/as the entanglements of quantum physics, where the one who knows, the measuring apparatus and the object to be interpreted are strangely involved.
Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation
Authors: Handa Shweta , Sharma Nivedita , Pathania Shruti
Published: May 2016

A novel strain Rhizopus sp. C4 was isolated from compost for the production of pectinase. Cultivation of Rhizopus sp. C4 on orange peel substrate under various solid-state fermentation (SSF) conditions was evaluated for pectinase yield along with the enzyme activity profile as a potential, low-cost alternative to submerged-liquid fermentation. Response surface methodology (RSM) was employed to optimize various environmental parameters for pectinase production. Various parameters, namely temperature, moisture and incubation days, were studied statistically for a total of 20 runs using central composite design. The highest yield of the enzyme, i.e., 11.63 IU/mL, was obtained from 1:3.5 moisture ratios in 7 days at 30°C. The study demonstrated that optimization through RSM could improve the enzymatic characteristics and yield of the enzyme.
Identification and Functional Analysis of Interleukin-1β in the Chinese Soft-Shelled Turtle Pelodiscus sinensis
Authors: Liang Quan , Li Weifen , Guo Ningning , Tong Chao , Zhou Yingshan , Fang Weihuan , Li Xiaoliang
Published: May 2016

Chinese soft-shelled turtle (Pelodiscus sinensis) is commercially cultured in East and Southeast Asia for its nutritional and medicinal values. In this study, we identified interleukin-1β (IL-1β) from Chinese soft-shelled turtle. The full-length cDNA of Pelodiscus sinensis IL-1β (tIL-1β) consists of 1529 base pairs with an 831-base-pair open reading frame, encoding 277 amino acids. The guanine-cytosine (GC) content in the coding sequence and 3’ untranslated region of tIL-1β is considerably higher than that of other vertebrates. Its mRNA expression level increased significantly during Aeromonas hydrophila infection. The tIL-1β lacks the typical IL-1β-converting enzyme (ICE) cut site found in mammalian IL-1β, but still could be cleaved by turtle caspase-1. By mutating the potential cleavage sites, we identified aspartic acid (Asp/D) 130 as the ICE cut site in tIL-1β. The peptide truncated at D130 was expressed using the baculovirus expression system; its bioactivity is confirmed by the ability to induce cyclooxygenase-2 (COX-2) and tIL-1β itself in peripheral blood monocytes. In conclusion, we characterized IL-1β from Chinese soft-shelled turtle and identified its D130 as a non-typical ICE cut size.
Paolo Mantegazza as Didatic Gastronome: Food, Art, Science and the New Italian Nation
Authors: De Feo Daniele
Published: May 2016

It is in Risorgimento Italy that there is an incessant quest for a definition of what it means to be Italian amongst a reality of economic paucity and clear social divisiveness. During this tenuous yet crucial epoch, there is a cohesive attempt to define Italian taste with an ideological terminology previously absent from sensorial and aesthetic discourse. A fundamental purveyor of this novel approach is the self-defined “poligamo delle scienze,” Paolo Mantegazza. To the plurality of roles attributed to the medic (anthropologist, pathologist, senator, writer, etc.), there is one yet to be explored—Mantegazza as didactic gastronome. In the attempt to combat what he considers the anti-hygienic conditions plaguing the nation, the medic inaugurates a pedagogic process that would ideally lead to the formation of the Italian citizen. With the goal of creating a stronger and more capable Italian populace, the author goes to great lengths to provide guidelines for maximizing nourishment through the humblest of foods. Ultimately, Mantegazza’s pedagogic gourmandism is integral in the propagation of a social model of comportment that defines the Positivist framework of biological and nationalistic renewal and to a new vision of taste.
Surface Runoff in Watershed Modeling—Turbulent or Laminar Flows?
Authors: Grismer Mark
Published: May 2016

Determination of overland sheet flow depths, velocities and celerities across the hillslope in watershed modeling is important towards estimation of surface storage, travel times to streams and soil detachment rates. It requires careful characterization of the flow processes. Similarly, determination of the temporal variation of hillslope-riparian-stream hydrologic connectivity requires estimation of the shallow subsurface soil hydraulic conductivity and soil-water retention (i.e., drainable porosities) parameters. Field rainfall and runoff simulation studies provide considerable information and insight into these processes; in particular, that sheet flows are likely laminar and that shallow hydraulic conductivities and storage can be determined from the plot studies. Here, using a 1 m by 2 m long runoff simulation flume, we found that for overland flow rates per unit width of roughly 30–60 mm2/s and bedslopes of 10%–66% with varying sand roughness depths that all flow depths were predicted by laminar flow equations alone and that equivalent Manning’s n values were depth dependent and quite small relative to those used in watershed modeling studies. Even for overland flow rates greater than those typically measured or modeled and using Manning’s n values of 0.30–0.35, often assumed in physical watershed model applications for relatively smooth surface conditions, the laminar flow velocities were 4–5 times greater, while the laminar flow depths were 4–5 times smaller. This observation suggests that travel times, surface storage volumes and surface shear stresses associated with erosion across the landscape would be poorly predicted using turbulent flow assumptions. Filling the flume with fine sand and conducting runoff studies, we were unable to produce sheet flow, but found that subsurface flows were onflow rate, soil depth and slope dependent and drainable porosities were only soil depth and slope dependent. Moreover, both the sand hydraulic conductivity and drainable porosities could be readily determined from measured capillary pressure displacement pressure head and assumption of pore-size distributions (i.e., Brooks-Corey lambda values of 2–3).
Simulation of Groundwater Contaminant Transport at a Decommissioned Landfill Site—A Case Study, Tainan City, Taiwan
Authors: Chen Chao-Shi , Tu Chia-Huei , Chen Shih-Jen , Chen Cheng-Chung
Published: May 2016

Contaminant transport in subsurface water is the major pathway for contamination spread from contaminated sites to groundwater supplies, to remediate a contaminated site. The aim of this paper was to set up the groundwater contaminant transport model for the Wang-Tien landfill site, in southwestern Taiwan, which exhibits high contamination of soil and groundwater and therefore represents a potential threat for the adjacent Hsu-Hsian Creek. Groundwater Modeling System software, which is the most sophisticated groundwater modeling tool available today, was used to numerically model groundwater flow and contaminant transport. In the simulation, the total mass of pollutants in the aquifer increased by an average of 72% (65% for ammonium nitrogen and 79% for chloride) after 10 years. The simulation produced a plume of contaminated groundwater that extends 80 m in length and 20 m in depth northeastward from the landfill site. Although the results show that the concentrations of ammonium nitrogen and chlorides in most parts are low, they are 3.84 and 467 mg/L, respectively, in the adjacent Hsu-Hsian Creek.
Twelfth International Symposium on Recent Advances in Environmental Health Research
Authors: Tchounwou Paul
Published: May 2016

During the past century, environmental hazards have become a major concern, not only to public health professionals, but also to the society at large because of their tremendous health, socio-cultural and economic impacts. Various anthropogenic or natural factors have been implicated in the alteration of ecosystem integrity, as well as in the development of a wide variety of acute and/or chronic diseases in humans. It has also been demonstrated that many environmental agents, acting either independently or in combination with other toxins, may induce a wide range of adverse health outcomes. Understanding the role played by the environment in the etiology of human diseases is critical to designing cost-effective control/prevention measures. This special issue of International Journal of Environmental Research and Public Health includes the proceedings of the Twelfth International Symposium on Recent Advances in Environmental Health Research. The Symposium provided an excellent opportunity to discuss the scientific advances in biomedical, environmental, and public health research that addresses global environmental health issues.
Minimal Functional Sites in Metalloproteins and Their Usage in Structural Bioinformatics
Authors: Rosato Antonio , Valasatava Yana , Andreini Claudia
Published: May 2016

Metal ions play a functional role in numerous biochemical processes and cellular pathways. Indeed, about 40% of all enzymes of known 3D structure require a metal ion to be able to perform catalysis. The interactions of the metals with the macromolecular framework determine their chemical properties and reactivity. The relevant interactions involve both the coordination sphere of the metal ion and the more distant interactions of the so-called second sphere, i.e., the non-bonded interactions between the macromolecule and the residues coordinating the metal (metal ligands). The metal ligands and the residues in their close spatial proximity define what we call a minimal functional site (MFS). MFSs can be automatically extracted from the 3D structures of metal-binding biological macromolecules deposited in the Protein Data Bank (PDB). They are 3D templates that describe the local environment around a metal ion or metal cofactor and do not depend on the overall macromolecular structure. MFSs provide a different view on metal-binding proteins and nucleic acids, completely focused on the metal. Here we present different protocols and tools based upon the concept of MFS to obtain deeper insight into the structural and functional properties of metal-binding macromolecules. We also show that structure conservation of MFSs in metalloproteins relates to local sequence similarity more strongly than to overall protein similarity.
Group Authentication Scheme for Neighbourhood Area Networks (NANs) in Smart Grids
Authors: Alohali Bashar , Kifayat Kashif , Shi Qi , Hurst William
Published: May 2016

A Neighbourhood Area Network is a functional component of the Smart Grid that interconnects the end user domain with the Energy Services Provider (ESP) domain. It forms the “edge” of the provider network, interconnecting homes instrumented with Smart Meters (SM) with the ESP. The SM is a dual interface, wireless communication device through which information is transacted across the user (a home) and ESP domains. The security risk to the ESP increases since the components within the home, interconnected to the ESP via the SM, are not managed by the ESP. Secure operation of the SM is a necessary requirement. The SM should be resilient to attacks, which might be targeted either directly or via the network in the home. This paper presents and discusses a security scheme for groups of SMs in a Neighbourhood Area Network that enable entire groups to authenticate themselves, rather than one at a time. The results show that a significant improvement in terms of resilience against node capture attacks, replay attacks, confidentiality, authentication for groups of SMs in a NAN that enable entire groups to authenticate themselves, rather than one at a time.
Three-Dimensional Fabrication for Microfluidics by Conventional Techniques and Equipment Used in Mass Production
Authors: Naito Toyohiro , Nakamura Makoto , Kaji Noritada , Kubo Takuya , Baba Yoshinobu , Otsuka Koji
Published: May 2016

This paper presents a simple three-dimensional (3D) fabrication method based on soft lithography techniques and laminated object manufacturing. The method can create 3D structures that have undercuts with general machines for mass production and laboratory scale prototyping. The minimum layer thickness of the method is at least 4 µm and bonding strength between layers is over 330 kPa. The performance reaches conventional fabrication techniques used for two-dimensionally (2D)-designed microfluidic devices. We fabricated some 3D structures, i.e., fractal structures, spiral structures, and a channel-in-channel structure, in microfluidic channels and demonstrated 3D microfluidics. The fabrication method can be achieved with a simple black light for bio-molecule detection; thus, it is useful for not only lab-scale rapid prototyping, but also for commercial manufacturing.
Plain Water and Sugar-Sweetened Beverage Consumption in Relation to Energy and Nutrient Intake at Full-Service Restaurants
Authors: An Ruopeng
Published: May 2016

Background: Drinking plain water, such as tap or bottled water, provides hydration and satiety without adding calories. We examined plain water and sugar-sweetened beverage (SSB) consumption in relation to energy and nutrient intake at full-service restaurants. Methods: Data came from the 2005–2012 National Health and Nutrition Examination Survey, comprising a nationally-representative sample of 2900 adults who reported full-service restaurant consumption in 24-h dietary recalls. Linear regressions were performed to examine the differences in daily energy and nutrient intake at full-service restaurants by plain water and SSB consumption status, adjusting for individual characteristics and sampling design. Results: Over 18% of U.S. adults had full-service restaurant consumption on any given day. Among full-service restaurant consumers, 16.7% consumed SSBs, 2.6% consumed plain water but no SSBs, and the remaining 80.7% consumed neither beverage at the restaurant. Compared to onsite SSB consumption, plain water but no SSB consumption was associated with reduced daily total energy intake at full-service restaurants by 443.4 kcal, added sugar intake by 58.2 g, saturated fat intake by 4.4 g, and sodium intake by 616.8 mg, respectively. Conclusion: Replacing SSBs with plain water consumption could be an effective strategy to balance energy/nutrient intake and prevent overconsumption at full-service restaurant setting.
Simulation of Jetting in Injection Molding Using a Finite Volume Method
Authors: Hua Shaozhen , Zhang Shixun , Cao Wei , Wang Yaming , Shao Chunguang , Liu Chuntai , Dong Binbin , Shen Changyu
Published: May 2016

In order to predict the jetting and the subsequent buckling flow more accurately, a three dimensional melt flow model was established on a viscous, incompressible, and non-isothermal fluid, and a control volume-based finite volume method was employed to discretize the governing equations. A two-fold iterative method was proposed to decouple the dependence among pressure, velocity, and temperature so as to reduce the computation and improve the numerical stability. Based on the proposed theoretical model and numerical method, a program code was developed to simulate melt front progress and flow fields. The numerical simulations for different injection speeds, melt temperatures, and gate locations were carried out to explore the jetting mechanism. The results indicate the filling pattern depends on the competition between inertial and viscous forces. When inertial force exceeds the viscous force jetting occurs, then it changes to a buckling flow as the viscous force competes over the inertial force. Once the melt contacts with the mold wall, the melt filling switches to conventional sequential filling mode. Numerical results also indicate jetting length increases with injection speed but changes little with melt temperature. The reasonable agreements between simulated and experimental jetting length and buckling frequency imply the proposed method is valid for jetting simulation.
Continent-Wide 2-D Co-Seismic Deformation of the 2015 Mw 8.3 Illapel, Chile Earthquake Derived from Sentinel-1A Data: Correction of Azimuth Co-Registration Error
Authors: Xu Bing , Li Zhiwei , Feng Guangcai , Zhang Zeyu , Wang Qijie , Hu Jun , Chen Xingguo
Published: May 2016

In this study, we mapped the co-seismic deformation of the 2015 Mw 8.3 Illapel, Chile earthquake with multiple Sentinel-1A TOPS data frames from both ascending and descending geometries. To meet the requirement of very high co-registration precision, an improved spectral diversity method was proposed to correct the co-registration slope error in the azimuth direction induced by multiple Sentinel-1A TOPS data frames. All phase jumps that appear in the conventional processing method have been corrected after applying the proposed method. The 2D deformation fields in the east-west and vertical directions are also resolved by combing D-InSAR and Offset Tracking measurements. The results reveal that the east-west component dominated the 2D displacement, where up to 2 m displacement towards the west was measured in the coastal area. Vertical deformations ranging between −0.25 and 0.25 m were found. The 2D displacements imply the collision of the Nazca plate squeezed the coast, which shows good accordance with the geological background of the region.
Satellite Retrievals of Karenia brevis Harmful Algal Blooms in the West Florida Shelf Using Neural Networks and Comparisons with Other Techniques
Authors: El-habashi Ahmed , Ioannou Ioannis , Tomlinson Michelle , Stumpf Richard , Ahmed Sam
Published: May 2016

We describe the application of a Neural Network (NN) previously developed by us, to the detection and tracking, of Karenia brevis Harmful Algal Blooms (KB HABs) that plague the coasts of the West Florida Shelf (WFS) using Visible Infrared Imaging Radiometer Suite (VIIRS) satellite observations. Previous approaches for the detection of KB HABs in the WFS primarily used observations from the Moderate Resolution Imaging Spectroradiometer Aqua (MODIS-A) satellite. They depended on the remote sensing reflectance signal at the 678 nm chlorophyll fluorescence band (Rrs678) needed for both the normalized fluorescence height (nFLH) and Red Band Difference algorithms (RBD) currently used. VIIRS which has replaced MODIS-A, unfortunately does not have a 678 nm fluorescence channel so we customized the NN approach to retrieve phytoplankton absorption at 443 nm (aph443) using only Rrs measurements from existing VIIRS channels at 486, 551 and 671 nm. The aph443 values in these retrieved VIIRS images, can in turn be correlated to chlorophyll-a concentrations [Chla] and KB cell counts. To retrieve KB values, the VIIRS NN retrieved aph443 images are filtered by applying limiting constraints, defined by (i) low backscatter at Rrs 551 nm and (ii) a minimum aph443 value known to be associated with KB HABs in the WFS. The resulting filtered residual images, are then used to delineate and quantify the existing KB HABs. Comparisons with KB HABs satellite retrievals obtained using other techniques, including nFLH, as well as with in situ measurements reported over a four year period, confirm the viability of the NN technique, when combined with the filtering constraints devised, for effective detection of KB HABs.
Nitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination
Authors: Ji Hanxu , Zhou Feng , Gu Jiangjiang , Shu Chen , Xi Kai , Jia Xudong
Published: May 2016

Nitrogen-doped carbon dots are introduced as a novel substrate suitable for enzyme immobilization in electrochemical detection metods. Nitrogen-doped carbon dots are easily synthesised from polyacrylamide in just one step. With the help of the amino group on chitosan, glucose oxidase is immobilized on nitrogen-doped carbon dots-modified carbon glassy electrodes by amino-carboxyl reactions. The nitrogen-induced charge delocalization at nitrogen-doped carbon dots can enhance the electrocatalytic activity toward the reduction of O2. The specific amino-carboxyl reaction provides strong and stable immobilization of GOx on electrodes. The developed biosensor responds efficiently to the presence of glucose in serum samples over the concentration range from 1 to 12 mM with a detection limit of 0.25 mM. This novel biosensor has good reproducibility and stability, and is highly selective for glucose determination under physiological conditions. These results indicate that N-doped quantum dots represent a novel candidate material for the construction of electrochemical biosensors.
Rapid Multi-Damage Identification for Health Monitoring of Laminated Composites Using Piezoelectric Wafer Sensor Arrays
Authors: Si Liang , Wang Qian
Published: May 2016

Through the use of the wave reflection from any damage in a structure, a Hilbert spectral analysis-based rapid multi-damage identification (HSA-RMDI) technique with piezoelectric wafer sensor arrays (PWSA) is developed to monitor and identify the presence, location and severity of damage in carbon fiber composite structures. The capability of the rapid multi-damage identification technique to extract and estimate hidden significant information from the collected data and to provide a high-resolution energy-time spectrum can be employed to successfully interpret the Lamb waves interactions with single/multiple damage. Nevertheless, to accomplish the precise positioning and effective quantification of multiple damage in a composite structure, two functional metrics from the RMDI technique are proposed and used in damage identification, which are the energy density metric and the energy time-phase shift metric. In the designed damage experimental tests, invisible damage to the naked eyes, especially delaminations, were detected in the leftward propagating waves as well as in the selected sensor responses, where the time-phase shift spectra could locate the multiple damage whereas the energy density spectra were used to quantify the multiple damage. The increasing damage was shown to follow a linear trend calculated by the RMDI technique. All damage cases considered showed completely the developed RMDI technique potential as an effective online damage inspection and assessment tool.
Suggestions of Policy Direction to Improve the Housing Quality in South Korea
Authors: Park Miyeon , Tae Sungho
Published: May 2016

Following the recent global climate changes, many countries, including developed nations, are announcing greenhouse gas (GHG) reduction targets and are actively participating in reducing GHG. Therefore, the role of the building sector on reducing GHG is being emphasized, and the establishment of policy structures for both making environmentally friendly capacity compulsory and improving the housing quality is being demanded. South Korea is also developing a policy for improving housing quality, but in order to achieve more competitive growth, this must be preceded by an analysis of current policy status within various countries on improving housing quality. This study aims to suggest direction about policies that will improve the housing quality in South Korea. For this, the policies of major countries were able to categorize and compare according to three major categories (performance, function, and aesthetics), and seven factors (safety, durability, cost and maintenance, response to residents’ needs, habitability, energy saving, and building design) regarding housing quality. As a result, from the performance aspect, policy directions were suggested for safe housing, the urban environment, regeneration of quality stock, and the usage of existing stock; from the functional aspect, policies for improving housing quality that responds to the aging population and energy saving housing were suggested; from the aesthetic aspect, housing designs that consider the urban environment were suggested.
Water Management Supporting the Delivery of Ecosystem Services for Grassland, Heath and Moorland
Authors: Ritzema Henk , Kirkpatrick Hilary , Stibinger Jakub , Heinhuis Hans , Belting Heinrich , Schrijver Raymond , Diemont Herbert
Published: May 2016

In the present era, permanent grasslands and other grazed habitats, i.e., moorlands and heath, are appreciated as avant la lettre green infrastructure (GI) resources, providing a wide range of ecosystem services, the delivery of many of which require water management to be in place. This paper discusses the role of water management and, in particular, that of drainage. We contend that controlled drainage and drainage-irrigation systems can contribute to the sustainable use of grasslands and associated habitats in the European Union. We present examples from a range of habitats in several EU Member States and attempt to identify the contemporary (short-term) costs as well as the short-term revenues covering these costs. Options for enhancing the role of the Green Infrastructure in Europe to achieve sustainable land use by including all “permanent grassland” are discussed.
Policy Mixes to Achieve Absolute Decoupling: A Case Study of Municipal Waste Management
Authors: Montevecchi Francesca
Published: May 2016

Studying the effectiveness of environmental policies is of primary importance to address the unsustainable use of resources that threatens the entire society. Thus, the aim of this paper is to investigate on the effectiveness of environmental policy instruments to decouple waste generation and landfilling from economic growth. In order to do so, the paper analyzes the case study of the Slovakian municipality of Palarikovo, which has drastically improved its waste management system between 2000 and 2012, through the utilization of differentiated waste taxes and awareness-raising and education campaigns, as well as targeting increased recycling and municipal composting. We find evidence of absolute decoupling for landfilled waste and waste generation, the latter being more limited in time and magnitude. These policy instruments could therefore play an important role in municipalities that are still lagging behind in waste management. More specifically, this policy mix was effective in moving away from landfilling, initiating recycling systems, and to some extent decreasing waste generation. Yet, a more explicit focus on waste prevention will be needed to address the entirety of the problem effectively.
Reduction in Primary Nonfunction of Syngeneic Islet Transplants with Nordihydroguaiaretic Acid, a Lipoxygenase Inhibitor
Authors: Hsu Brend Ray-Sea , Juang Jyuhn-Huarng , Fu Shin-Huei , Kuo Chien-Hung , Lu Wen-Tsoung
Published: May 2016

To study the effectiveness of a lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA), in the reduction of primary nonfunction, an insufficient number of syngeneic islets were transplanted underneath the renal capsule with NDGA administered daily for 4 weeks. After transplantation of the 150 islets, the decrement of blood glucose levels was significantly faster in the mice that had received NDGA than in the mice that had received no drug at all or dimethyl sulfoxide (DMSO) (p < 0.005, p < 0.05). The mean duration of temporary posttransplant hyperglycemia was 22.3 ± 3.2 (n = 10), 35.9 ± 2.3 (n = 14), and 33.7 ± 4.1 (n = 6) days for the respective groups. The diabetic mice that received 300 islets had their blood glucose levels decrease faster than those that received 150 islets (19.7 ± 1.6 vs. 35.9 ± 2.3 days, n = 14, p < 0.0001). There was no significant difference in the blood glucose reducing effect between the mice that received 150 islets with NDGA and the mice that received 300 islets [22.3 ± 3.2 (n = 10) vs. 19.7 ± 1.6 (n = 14) days, p > 0.05]. The insulin content of the graft from the mice treated with 150 islets and NDGA (3.02 ± 0.24 μg, n = 4) was higher than that from the mice that received 150 islets but no treatment (1.10 ± 0.26 μg, n = 15, p < 0.005) or that had been treated with DMSO (1.21 ± 0.30 μg, n = 4, p < 0.05). The insulin content of the pancreas remnant had no significant differences among the three groups. The net glucose-stimulated insulin secretion was 0.82 ± 0.14 vs. 0.20 ± 0.10 μIU/islet × 60 min (n = 8, p < 0.005) and 0.59 ± 0.08 vs. 0.04 ± 0.02 μIU/islet × 60 min (n = 8, p < 0.0001) for islets cultured without NDGA vs. with NDGA at 1 and 2 weeks, respectively. However, the insulin content of the cultured islets was similar between the two groups for up to 2 weeks of incubation (at 1 week: 0.71 ± 0.01 vs. 0.67 ± 0.04 ng/islet, n = 8, p > 0.05; at 2 weeks: 0.71 ± 0.02 vs. 0.80 ± 0.07 ng/islet, n = 8, p > 0.05). Serum leukotriene B4 (LTB4) concentrations before and between the fifth and seventh days after transplantation were determined. For diabetic mice that received 150 islets, serum LTB4 levels were 25,835 ± 3,335 and 27,631 ± 3,136 pg/ml (n = 4, p > 0.05). For diabetic mice that received 150 islets and NDGA, the corresponding figures were 22,401 ± 2,706 pg/ml and 27,530 ± 2,190 pg/ml (n = 8, p > 0.05). The graft histology revealed viable islet cells and networks of close vascular structures around the islets and did not reveal microscopic differences among the samples of all four groups. In conclusion, our data revealed that daily administration of NDGA for 4 weeks enhanced isoislet engraftment and preserved three times more mass of the islet beta cells in the isografts. This result indicates that NDGA reduces primary nonfunction of islet syngeneic grafts in diabetic mice.
Islet Cryopreservation: Improved Recovery following Taurine Pretreatment
Authors: Hardikar Anandwardhan A. , Risbud Makarand V. , Remacle Claude , Reusens Brigitte , Hoet Joseph J. , Bhonde Ramesh R.
Published: May 2016

Simple and efficient freezing methods with maximal postthawing recovery form the basis of ideal cryopreservation. Taurine (2-amino ethanesulfonic acid), an end-product of sulphur amino acid metabolism, is one of the most abundant free amino acids in the body. The membrane stabilizing, free radical scavenging, and osmoregulatory roles of taurine have been well documented. We studied the effect of physiological and supra-physiological concentrations (0.3 and 3.0 mM) of taurine on islet cryopreservation. Islet viability on cryopreservation was significantly improved in both the taurine-treated groups (91.9 ± 2.3% in 0.3 mM and 94.6 ± 1.58% in 3.0 mM group, p < 0.05) compared with the controls (85.7 ± 3.4%). Loss of peripheral islet cells was highly reduced in the taurine group, as examined under phase contrast and quantified by islet morphometric analysis (p < 0.05) using a digital image analysis system. Taurine-treated islets showed significant reduction in lipid peroxidation (0.905 and 0.848 nM MDA/μg protein for 0.3 and 3.0 mM taurine, respectively, p < 0.05) compared with control (1.307 nM MDA/μg protein) islets. In all, 500 islet equivalents (IE) of treated or control group islets were transplanted to BALB/c mice rendered diabetic with STZ. All animals showed a normal glucose clearance following a glucose load. Graft functionality was confirmed by normoglycemia (fasting plasma glucose: fpg < 150 mg/dl) after transplantation and reappearing hyperglycemia (fpg > 200 mg/dl) following removal of the graft. Suboptimal islet transplantation using 250 IE suggests that the grafted islet mass was inadequate for diabetes reversal. In addition, no significant differences were observed in the islet insulin content between the three groups following cryopreservation of the islets at −196°C. Our studies indicate that taurine pretreatment and its continued presence during islet cryopreservation improves the postthawing viable recovery of islets.
Enhanced Survival of Porcine Neural Xenografts in Mice Lacking CD1d1, But No Effect of NK1.1 Depletion
Authors: Larsson Lena C. , Anderson Per , Widner Håkan , Korsgren Olle
Published: May 2016

Transplantation of embryonic porcine neurons may restore neurological function in patients with Parkinson's disease, if immunological rejection could be prevented. This study was performed to investigate the role of natural killer cells (NK cells) and NK1.1+ T cells (NK T cells) in the rejection of neural xenografts. A cell suspension was prepared from the ventral mesencephalon of 26 – 27-day-old pig embryos, and 2 μl was implanted in the right striata of mutant CD1d1 null (CD1.1-/-) mice, NK1.1-depleted mice, and controls. The CD1.1-/- mice are deficient in NK T cells and the antigen-presenting molecule CD1d1. Graft survival and host responses were determined immunohistochemically using markers for dopamine neurons, CD4-, CD8- cells, microglia, and macrophages. At 2 weeks, the grafts were significantly larger in CD1.1-/- mice, 0.09 ± 0.02 μl (mean ± SEM), compared with controls, 0.05 ± 0.01 μl. There was no significant difference between NK1.1-depleted mice, 0.02 ± 0.01 μl, and controls. At 5 weeks, two grafts were still present in the CD1-/- mice, whereas only scars remained in the controls and in the NK1.1-depleted mice. Immune reactions were strong at 2 weeks and less pronounced at 5 weeks in all groups. Microglial activation was lower in NK-depleted mice than in the controls at 2 weeks. In contrast to organ xenografting, NK1.1+ cells do not seem to be important mediators of the rejection of discordant cellular neural xenografts. However, our results suggest that the antigen-presenting molecule CD1d1 may be involved in the rejection process.
Probing Enhanced Cytochrome P450 2B1/2 Activity in Rat Hepatocyte Spheroids through Confocal Laser Scanning Microscopy
Authors: Tzanakakis Emmanouhl S. , Hsiao Chang-Chun , Matsushita Taku , Remmel Rory P. , Hu Wei-Shou
Published: May 2016

Cytochrome P450 (CYP450) enzymes are essential for xenobiotic metabolism. Although CYP450s are found in many tissues, CYP2B1/2 are primarily expressed in the rat liver. The constitutive expression in vivo of CYP2B1/2 is low but it is induced in the presence of various drugs such as phenobarbital (PB). In this study, CYP2B1/2 activity in cultured hepatocytes was assessed in situ with the introduction of a fluorogenic sub-strate, pentoxyresorufin. The product of 7-pentoxyresorufin-O-dealkylation (PROD), which is catalyzed specifically by CYP2B1/2, was detected using confocal laser scanning microscopy (CLSM). Primary hepatocytes cultured as monolayers on collagen-coated surfaces exhibited background PROD activity and minimal PB inducibility after 4 days in culture. In contrast, rat hepatocytes organized in compacted aggregates, or spheroids, exhibited higher levels of PROD activity and retained their ability for PB induction. The results from the CLSM analysis were verified by RT-PCR and Western immunoblotting analysis. Furthermore, CLSM in conjunction with image processing techniques and three-dimensional reconstruction revealed the localization of enhanced PROD activity in the center of spheroids. The results support the use of CLSM as a powerful tool for investigating CYP2B1/2 activity in cultured rat hepatocytes.
Intracerebral Transplantation and Successful Integration of Astrocytes following Genetic Modification with a High-Capacity Adenoviral Vector
Authors: Arnhold Stefan , Kreppel F. , Kandirali S. , Lenartz D. , Klinz F.-J. , Sturm V. , Kochanek S. , Andressen C. , Addicks K.
Published: May 2016

To investigate the ability of genetically modified astrocytes to integrate into adult rat brain, two spontaneously immortalized cell lines and the allogenic nontumorigenic glioma cell line F98 were transduced with a high-capacity adenoviral vector (HC-Adv) expressing the EGFP gene from the hCMV promoter. In organotypic slice cultures the transduced astrocytes were shown to integrate into the brain tissue. Following transplantation of the transduced astrocytes into the striatum of adult rats, the transplanted cells survived at least for 6 weeks, continuously expressed the EGFP transgene, in close neighborhood with cells of the recipient tissue executing their differentiation capacity along the glial lineage. Thus, HC-Adv transduced astrocytes are promising vehicles to locally deliver therapeutic proteins for the treatment of neurodegenerative diseases.
Monkey Embryonic Stem Cell Lines Expressing Green Fluorescent Protein
Authors: Takada Tatsuyuki , Suzuki Yutaka , Kondo Yasushi , Kadota Nae , Kobayashi Kinji , Nito Shinji , Kimura Hiroshi , Torii Ryuzo
Published: May 2016

The major limitation of nonhuman primate (NHP) embryonic stem (ES) cell research is inefficient genetic modification and limited knowledge of differentiation mechanisms. A genetically modified NHP-ES cell with biomarkers, such as green fluorescent protein (GFP), that allow noninvasive monitoring of transgenic cells, is a useful tool to study cell differentiation control during preimplantation and fetal development, which also plays a crucial role in the development of cell transplantation medicine. Here we report the establishment of transgenic NHP-ES cell lines that express GFP without jeopardizing their pluripotency, which was confirmed by in vitro and in vivo differentiation. These GFP-expressing ES cells reproducibly differentiated into embryoid bodies, neural cells, and cardiac myocytes. They formed teratoma composed of tissues derived from the three embryonic germ layers when transplanted into severe combined immunodeficient disease (SCID) mice. GFP expression was maintained in these differentiated cells, suggesting that these cells were useful for cell transplantation experiments. Furthermore, we showed that these ES cells have the ability to form chimeric blastocysts by introducing into the early preimplantation stage NHP embryo.
Dynamics of the Early Immune Cellular Reactions after Myogenic Cell Transplantation
Authors: Skuk Daniel , Caron Nicolas , Goulet Marlyne , Roy Brigitte , Espinosa Francisco , Tremblay Jacques P.
Published: May 2016

The role of immune cells in the early donor cell death/survival following myoblast transplantation is confusing, one of the reasons being the lack of data about the immune reactions following cell transplantation. We used outbred mice as hosts for transplantation of primary cultured muscle cells and T-antigen-immortalized myoblasts. The host muscles were analyzed 1 h to 7 days after cell injection. No net loss of the donor primary cultured cell population was observed in this period. The immune cellular reaction in this case was: 1) a brief (<48 h) neutrophil invasion; 2) macrophage infiltration from days 1 to 7; 3) a specific response involving CTL and few NK cells (days 6 and 7), preceded by a low CD4+ cell infiltration starting at day 3. In contrast, donor-immortalized myoblasts completely disappeared during the 7-day follow-up. In this case, an intense infiltration of CTL and macrophages, with moderate CD4+ infiltration and lower amounts of NK cells, was observed starting at day 2. The nonspecific immune response at days 0 and 1 was similar for both types of donor cells. The present observations set a basis to interpret the role of immune cells on the early death/survival of donor cells following myoblast transplantation.
The Release of Excitatory Amino Acids, Dopamine, and Potassium following Transplantation of Embryonic Mesencephalic Dopaminergic Grafts to the Rat Striatum, and Their Effects on Dopaminergic Neuronal Survival In Vitro
Authors: Zietlow Rike , Sinclair Simon R. , Schwiening Christof J. , Dunnett Stephen B. , Fawcett James W.
Published: May 2016

A major limitation to the effectiveness of grafts of fetal ventral mesencephalic tissue for parkinsonism is that about 90–95% of grafted dopaminergic neurones die. In rats, many of the cells are dead within 1 day and most cell death is complete within 1 week. Our previous results suggest that a major cause of this cell death is the release of toxins from the injured CNS tissue surrounding the graft, and that many of these toxins have dissipated within 1 h of inserting the grafting cannula. In the present experiments we measured the change over time in the concentration of several potential toxins around an acutely implanted grafting cannula. We also measured the additional effect of injecting suspensions of embryonic mesencephalon, latex microspheres, or vehicle on these concentrations. Measurements of glutamate, aspartate, and dopamine by microdialysis showed elevated levels during the first 20–60 min, which then declined to baseline. In the first 20 min glutamate levels were 10.7 times, aspartate levels 5 times, and dopamine levels 24.3 times baseline. Potassium levels increased to a peak of 33 ± 10.6 mM 4–5 min after cannula insertion, returning to baseline of <5 mM by 30 min. Injection of cell suspension, latex microspheres, or vehicle had no significant effect on these levels. We then assayed the effect of high concentrations of glutamate, aspartate, dopamine, and potassium on dopaminergic neuronal survival in E14 ventral mesencephalic cultures. In monolayer cultures only dopamine at 200 μM showed toxicity. In three-dimensional cultures only the combination of raised potassium, dopamine, glutamate, and aspartate together decreased dopaminergic neuronal survival. We conclude that toxins other than the ones measured are the main cause of dopaminergic cell death after transplantation, or the effects of the toxins measured are enhanced by anoxia and metabolic challenges affecting newly inserted grafts.
Anoikis: Roadblock to Cell Transplantation?
Authors: Zvibel Isabel , Smets Françoise , Soriano Humberto
Published: May 2016

Cell therapy, in particular liver cell transplantation, holds great therapeutic potential and is partially hindered by the high rate of apoptosis during cell isolation, cryopreservation, and engraftment. Apoptosis occurring due to cell detachment from the extracellular matrix is a phenomenon termed “anoikis. ” The purpose of this review is to describe signaling mechanisms pertinent to anoikis in both immortalized cell lines, but particularly in primary normal epithelial cells. The mechanisms described include integrin signaling and survival molecules, caspase activation, and the role of mitochondrial proteins in anoikis. Strategies to prevent anoikis during isolation and cryopreservation of hepatocytes are discussed.