bluuebunny/crossref_metadata_2025 huggingface dataset:
Cationic-Surfactant (CTAB) Assisted Preparation of 2D Graphitic Carbon Nitride (g-C3N4) Sheets Advances Supercapacitive Performance
Authors: Mane Sagar M. , Teli Aviraj M. , Beknalkar Sonali A. , Patil Deepak R. , Shin Jae Cheol , Lee Jaewoong
Published: March 2024

The distinct physicochemical characteristics of metal-free graphitic carbon nitride (g-C3N4) are gaining interest in various fields, including energy storage and conversion. However, the electrochemical performance of this material is constrained, owing to its minimal surface area. Incorporating a surfactant is one of the ways to resolve the issue of surface area and therefore improve the electrochemical performance of g-C3N4. This research delves into a method aimed at improving the supercapacitive capabilities of 2D g-C3N4 sheets through the implementation of a cationic surfactant, cetyltrimethylammonium bromide (CTAB). Electrochemical studies reveal that the CTAB-assisted g-C3N4 sheets exhibit remarkable improvements in specific capacitance, cyclic stability, and comparative rate capability in relation to pristine g-C3N4. The specific capacitance of g-C3N4 with CTAB exceeds about 28%, which gives 162. 8 F g−1. This value is 117.7 F g−1 for electrode material without CTAB at 0.5 mA cm−2. This improved electrochemical performance can be credited to the heightened surface area, improved electronic conductivity, and optimized charge transfer kinetics facilitated by the CTAB surfactant. We aim to emphasize the enhancement of the overall performance of g-C3N4-based supercapacitors for advanced energy storage systems.
Deterioration of Performance Status during Palliative Radiotherapy Suggests a Significant Short Survival Duration: Indicating the Necessities for Considering Radiotherapy Discontinuation
Authors: Maemoto Hitoshi , Kushi Kazuaki , Owan Isoko , Ariga Takuro , Heianna Joichi , Nishie Akihiro
Published: March 2024

Discontinuation of palliative radiotherapy due to a patient’s declining general condition poses a clinical dilemma for palliative care physicians. This study aimed to investigate the survival duration of patients whose performance status (PS) deteriorated during palliative radiotherapy and inform decisions regarding early treatment discontinuation. We retrospectively analyzed data from patients referred from our institute’s palliative care department who underwent ≥10 fractions of palliative radiotherapy between March 2017 and December 2021. PS was assessed using the Eastern Cooperative Oncology Group (ECOG) scale. Survival duration was calculated from the final day of palliative radiotherapy to death using the Kaplan–Meier method. A total of 35 patients underwent palliative radiotherapy. Seven (20%) experienced deterioration in ECOG PS during treatment. Their median survival duration was significantly shorter at 22 days (95% confidence interval: 1–94 days) compared to 125 days (95% confidence interval: 82–150 days) for the 28 patients whose PS remained stable (p = 0.0007). Deterioration in ECOG PS during palliative radiotherapy signifies a markedly shorter survival duration. Careful assessment of a patient’s condition throughout treatment is crucial, and early discontinuation should be considered if their general health worsens rather than strictly adhering to the initial schedule.
Metarhizium dianzhongense sp. nov. and New Record of M. bibionidarum (Clavicipitaceae, Hyocreales) Attacking Insects from China
Authors: Wei Cui-Yuan , Tang Mei , Xie Liu-Yi , Fan Qi , Shen Shi-Kang , Yang Zhu-Liang , Deng Gang , Wang Yuan-Bing
Published: March 2024

The genus Metarhizium is one of the most significant entomopathogenic fungi with diverse morphological characteristics and host species. Species of Metarhizium have been widely used for pest control as an environmentally safe alternative to chemical pesticides. This study reports a new species of Metarhizium and a new record of M. bibionidarum from China. The taxonomic positions of the two species within Metarhizium were assessed by morphological and multi-gene phylogenetic data. This assessment confirmed that the new species M. dianzhongense on white grubs (Coleoptera) is a sister to M. ellipsoideum on adult leafhoppers (Hemiptera) and represents a distinctive fungus according to the morphological and phylogenetic evidence. The two species, M. dianzhongense and M. bibionidarum, were described and illustrated. Pathogenicity tests by M. bibionidarum and M. dianzhongense were performed on early instar larvae of the significant agricultural pest Spodoptera frugipera (Lepidoptera). The results demonstrated that both M. bibionidarum and M. dianzhongense exhibit significant insecticidal activity against larvae of S. frugipera, providing new fungal resources for the development of an eco-friendly biocontrol agent against this pest.
Biodiversity of Gelatinous Organisms in the Western Adriatic Sea and Identification of Their Echo Traces in Acoustic Data
Authors: De Felice Andrea , Biagiotti Ilaria , Canduci Giovanni , Costantini Ilaria , Palermino Antonio , Centurelli Michele , Menicucci Samuele , Leonori Iole
Published: March 2024

The abundance of gelatinous organisms, such as salps and jellyfish, in the Adriatic Sea has significantly increased over the past decade. Environmental factors play a key role in driving this shift in abundance through rising temperatures and a consequent decrease in oxygen levels in the water, for which jellyfish have higher tolerance levels. Additionally, fisheries may contribute to the proliferation of jellyfish by diminishing their natural predators and food competitors. Pelagic trawl catch data from 2015 to 2023 acquired during MEDIAS acoustic surveys in the western Adriatic Sea were reviewed to extract information concerning the abundance and distribution of salps and jellyfish. These data were subsequently analyzed and compared with satellite environmental information to identify potential correlations. When considering environmental information related to the month of the survey, the results show two significant relationships: one between the abundance of Aequorea aequorea and average salinity and another one between the abundance of Rhizostoma pulmo and bottom temperature. Furthermore, when considering environmental data from the month preceding the survey, a relationship between the overall abundance of gelatinous organisms, salps and jellyfish together, and surface temperature was identified. Additionally, an analysis was conducted on specific hauls that almost exclusively yielded jellyfish, with the aim of identifying their echo traces. Although it was not possible to allocate one jellyfish species to a specific echo trace due to the frequent co-occurrence of more than one species, a general indication of typical backscatter for these species, with a higher response at 70 kHz, was consistently observed in all cases examined.
Zilucoplan: A Newly Approved Macrocyclic Peptide for Treatment of Anti-Acetylcholine Receptor Positive Myasthenia Gravis
Authors: Costa Lia , Fernandes Carla
Published: March 2024

Zilucoplan is a synthetic macrocyclic peptide approved by the Food and Drug Administration (FDA), in October 2023, for the treatment of generalized myasthenia gravis. It is considered as an orphan drug that causes the inhibition of terminal complement cascade activation with a dual mechanism of action preventing the formation of the membrane attack complex (MAC) and the destruction of the neuromuscular junction. This drug has been demonstrated to be able to treat the generalized myasthenia gravis without significant adverse effects, with good efficacy, safety, and tolerability profile. Zilucoplan is not only innovative and promising in the therapeutics of generalized myasthenia gravis, but it could also be beneficial for the treatment of other diseases as well as a model for synthesis of analogues to improve pharmacological profile.
Phenotypes and Serum Biomarkers in Sarcoidosis
Authors: Della Zoppa Matteo , Bertuccio Francesco Rocco , Campo Ilaria , Tousa Fady , Crescenzi Mariachiara , Lettieri Sara , Mariani Francesca , Corsico Angelo Guido , Piloni Davide , Stella Giulia Maria
Published: March 2024

Sarcoidosis is a multisystem disease, which is diagnosed on a compatible clinical presentation, non-necrotizing granulomatous inflammation in one or more tissue samples, and exclusion of alternative causes of granulomatous disease. Considering its heterogeneity, numerous aspects of the disease remain to be elucidated. In this context, the identification and integration of biomarkers may hold significance in clinical practice, aiding in appropriate selection of patients for targeted clinical trials. This work aims to discuss and analyze how validated biomarkers are currently integrated in disease category definitions. Future studies are mandatory to unravel the diverse contributions of genetics, socioeconomic status, environmental exposures, and other sociodemographic variables to disease severity and phenotypic presentation. Furthermore, the implementation of transcriptomics, multidisciplinary approaches, and consideration of patients’ perspectives, reporting innovative insights, could be pivotal for a better understanding of disease pathogenesis and the optimization of clinical assistance.
Irruption and Absorption: A ‘Black-Box’ Framework for How Mind and Matter Make a Difference to Each Other
Authors: Froese Tom
Published: March 2024

Cognitive science is confronted by several fundamental anomalies deriving from the mind–body problem. Most prominent is the problem of mental causation and the hard problem of consciousness, which can be generalized into the hard problem of agential efficacy and the hard problem of mental content. Here, it is proposed to accept these explanatory gaps at face value and to take them as positive indications of a complex relation: mind and matter are one, but they are not the same. They are related in an efficacious yet non-reducible, non-observable, and even non-intelligible manner. Natural science is well equipped to handle the effects of non-observables, and so the mind is treated as equivalent to a hidden ‘black box’ coupled to the body. Two concepts are introduced given that there are two directions of coupling influence: (1) irruption denotes the unobservable mind hiddenly making a difference to observable matter, and (2) absorption denotes observable matter hiddenly making a difference to the unobservable mind. The concepts of irruption and absorption are methodologically compatible with existing information-theoretic approaches to neuroscience, such as measuring cognitive activity and subjective qualia in terms of entropy and compression, respectively. By offering novel responses to otherwise intractable theoretical problems from first principles, and by doing so in a way that is closely connected with empirical advances, irruption theory is poised to set the agenda for the future of the mind sciences.
A Unifying Generator Loss Function for Generative Adversarial Networks
Authors: Veiner Justin , Alajaji Fady , Gharesifard Bahman
Published: March 2024

A unifying α-parametrized generator loss function is introduced for a dual-objective generative adversarial network (GAN) that uses a canonical (or classical) discriminator loss function such as the one in the original GAN (VanillaGAN) system. The generator loss function is based on a symmetric class probability estimation type function, Lα, and the resulting GAN system is termed Lα-GAN. Under an optimal discriminator, it is shown that the generator’s optimization problem consists of minimizing a Jensen-fα-divergence, a natural generalization of the Jensen-Shannon divergence, where fα is a convex function expressed in terms of the loss function Lα. It is also demonstrated that this Lα-GAN problem recovers as special cases a number of GAN problems in the literature, including VanillaGAN, least squares GAN (LSGAN), least kth-order GAN (LkGAN), and the recently introduced (αD,αG)-GAN with αD=1. Finally, experimental results are provided for three datasets—MNIST, CIFAR-10, and Stacked MNIST—to illustrate the performance of various examples of the Lα-GAN system.
Finite-Time H∞ Controllers Design for Stochastic Time-Delay Markovian Jump Systems with Partly Unknown Transition Probabilities
Authors: Guo Xinye , Li Yan , Liu Xikui
Published: March 2024

This paper concentrates on the finite-time H∞ control problem for a type of stochastic discrete-time Markovian jump systems, characterized by time-delay and partly unknown transition probabilities. Initially, a stochastic finite-time (SFT) H∞ state feedback controller and an SFT H∞ observer-based state feedback controller are constructed to realize the closed-loop control of systems. Then, based on the Lyapunov–Krasovskii functional (LKF) method, some sufficient conditions are established to guarantee that closed-loop systems (CLSs) satisfy SFT boundedness and SFT H∞ boundedness. Furthermore, the controller gains are obtained with the use of the linear matrix inequality (LMI) approach. In the end, numerical examples reveal the reasonableness and effectiveness of the proposed designing schemes.
Software-Defined Time-Sensitive Networking for Cross-Domain Deterministic Transmission
Authors: Guo Mengjie , Shou Guochu , Liu Yaqiong , Hu Yihong
Published: March 2024

With the rapid development of Industrial 4.0, massive emerging time-critical applications pose new demands for industrial networks, such as strict latency boundaries, ultra-reliable transmission, and so on. Time-Sensitive Networking (TSN) is well suited for these demanding scenarios due to its support for deterministic transmission based on Ethernet. However, many use cases in real industrial scenarios involve non-TSN domains, and, thus, the network requires providing deterministic transmission across non-TSN domains to support them. To this end, this paper proposes a novel Software-Defined Time-Sensitive Networking (SD-TSN) framework that integrates the determinism of TSN and scalability of Software-Defined Networking (SDN). SD-TSN exploits the coordination between the coordinated controller and different domain controllers to bound non-deterministic queuing delays in a way that guarantees determinism. In particular, we designed a multi-domain time-aware traffic scheduling model, which harmonizes the time-aware shaper (TAS) in TSN and time-slots in non-TSN domains based on a global view, generating transmission schedules for each domain. A prototype testbed was built to conduct the experiments. The evaluation results demonstrate that the proposed method can efficiently achieve bounded delay and low delay variations in the transmission across non-TSN domains.
Retrofitting of an Existing Cultural Hall into a Net-Zero Energy Building
Authors: Constantinides Andreas , Katafygiotou Martha , Dimopoulos Thomas , Kapellakis Iosif
Published: March 2024

The energy efficiency of existing buildings is a crucial factor in addressing energy consumption challenges in European countries, accounting for nearly 40% of the total energy usage. One such country is Cyprus, which faces significant challenges in transforming its existing building stock into energy-efficient and sustainable structures. To face this situation, extensive focus has been made by the government on the energy-efficient retrofit of non-residential public buildings erected before 2010, which lack any energy efficiency measures. This study examines the case of the Pano Polemidia Cultural Hall (PPCH), which represents the building stock of that period. Through the simulation of two scenarios, before and after the adoption of retrofit measures, the existing energy performance is initially evaluated and then the adoption of sustainable solutions, which improve substantially the energy efficiency and can be easily adopted from the relevant authorities, is explored. These retrofit measures include installation of HVAC system, covering of the shell of the building with external thermal insulation, lighting replacement with LED devices, installation of PV system and solar panels, and replacement of the external openings with aluminum windows. The results derived show that the energy consumption of the building was reduced from 468 to 218 kWh/m2·yr, with renewable energy sources (RESs) contributing 177 kWh/m2·yr, the CO2 emissions were reduced from 136.73 to 11.5 kg/m2·yr, while the reduction in energy consumption per sector ranged from 25% in lighting to 83% in hot water. Therefore, it is evident that a comprehensive retrofitting plan can transform the PPCH into a near-zero energy consumption building that also provides value to the local community and can act as a successful example for any other non-residential buildings with similar characteristics.
Divergent Nitrogen, Phosphorus, and Carbon Concentrations among Growth Forms, Plant Organs, and Soils across Three Different Desert Ecosystems
Authors: Khan Alamgir , Liu Xu-Dong , Waseem Muhammad , Qi Shi-Hua , Ghimire Shantwana , Hasan Md. Mahadi , Fang Xiang-Wen
Published: March 2024

Quantifying the dryland patterns of plant carbon (C), nitrogen (N), and phosphorus (P) concentrations and their stoichiometric values along environmental gradients is crucial for understanding ecological strategies. To understand the plant adaptive strategies and ecosystem nutrient concentrations across three desert ecosystems (e.g., desert, steppe desert, and temperate desert), we compiled a dataset consisting of 1295 plant species across three desert ecosystems. We assessed the element concentrations and ratios across plant growth forms, plant organs, and soils and further analysed the leaf vs. root N, P, and N:P scaling relationships. We found that the leaf N, P, and C concentrations were significantly different only from those of certain other growth forms and in certain desert ecosystems, challenging the generality of such differences. In leaves, the C concentrations were always greater than the N and P concentrations and were greater than those in soils depending on the soil chemistry and plant physiology. Thus, the element concentrations and ratios were greater in the organs than in the soils. The values in the leaf versus the root N, P, and N:P scaling relationships differed across the three desert ecosystems; for example, αN (1.16) was greater in the desert, αP (1.10) was greater in the temperate desert ecosystem, and αN:P (2.11) was greater in the desert ecosystem. The mean annual precipitation (MAP) and mean annual temperature (MAT) did not have significant effects on the leaf elemental concentrations or ratios across the desert ecosystems. This study advances our understanding of plant growth forms and organs, which support resource-related adaptive strategies that maintain the stability of desert ecosystems via divergent element concentrations and environmental conditions.
Flavor Chemical Research on Different Bee Pollen Varieties Using Fast E-Nose and E-Tongue Technology
Authors: Liu Chenshuo , Zhou Enning , Zhu Yuying , Li Qiangqiang , Wu Liming
Published: March 2024

Bee pollen, derived from various plant sources, is renowned for its nutritional and bioactive properties, aroma, and taste. This study examined the bee pollen with the highest yield in China obtained from four plant species, namely Brassica campestris (Bc), Nelumbo nucifera (Nn), Camellia japonica (Cj), and Fagopyrum esculentum (Fe), using fast e-nose and e-tongue technology to analyze their flavor chemistry. Results showed substantial differences in scent profiles among the varieties, with distinct odor compounds identified for each, including n-butanol, decanal, and ethanol, in Bc, Nn, and Cj, respectively. The primary odorants in Fe consist of E-2-hexen-1-ol and (Z)-3-hexen-1-ol. Additionally, e-tongue analysis revealed seven distinct tastes in bee pollen samples: AHS, PKS, CTS, NMS, CPS, ANS, and SCS, with variations in intensity across each taste. The study also found correlations between taste components and specific odor compounds, providing insights for enhancing product quality control in bee pollen processing.
Resistant Starch from Purple Sweet Potatoes Alleviates Dextran Sulfate Sodium-Induced Colitis through Modulating the Homeostasis of the Gut Microbiota
Authors: Wang Zhihao , Gao Min , Kan Juan , Cheng Qingyang , Chen Xiaotong , Tang Chao , Chen Dan , Zong Shuai , Jin Changhai
Published: March 2024

Ulcerative colitis (UC) is a complicated inflammatory disease with a continually growing incidence. In this study, resistant starch was obtained from purple sweet potato (PSPRS) by the enzymatic isolation method. Then, the structural properties of PSPRS and its protective function in dextran sulfate sodium (DSS)-induced colitis were investigated. The structural characterization results revealed that the crystallinity of PSPRS changed from CA-type to A-type, and the lamellar structure was totally destroyed during enzymatic hydrolysis. Compared to DSS-induced colitis mice, PSPRS administration significantly improved the pathological phenotype and colon inflammation in a dose-dependent manner. ELISA results indicated that DSS-induced colitis mice administered with PSPRS showed higher IL-10 and IgA levels but lower TNF-α, IL-1β, and IL-6 levels. Meanwhile, high doses (300 mg/kg) of PSPRS significantly increased the production of acetate, propionate, and butyrate. 16S rDNA high-throughput sequencing results showed that the ratio of Firmicutes to Bacteroidetes and the potential probiotic bacteria levels were notably increased in the PSPRS treatment group, such as Lactobacillus, Alloprevotella, Lachnospiraceae_NK4A136_group, and Bifidobacterium. Simultaneously, harmful bacteria like Bacteroides, Staphylococcus, and Akkermansia were significantly inhibited by the administration of a high dose of PSPRS (p < 0.05). Therefore, PSPRS has the potential to be a functional food for promoting intestinal health and alleviating UC.
Bifurcation Exploration and Controller Design in a Fractional Oxygen–Plankton Model with Delay
Authors: Zhang Yunzhang , Xu Changjin
Published: March 2024

Fractional-order differential equations have been proved to have great practical application value in characterizing the dynamical peculiarity in biology. In this article, relying on earlier work, we formulate a new fractional oxygen–plankton model with delay. First of all, the features of the solutions of the fractional delayed oxygen–plankton model are explored. The judgment rules on non-negativeness, existence and uniqueness and the boundedness of the solution are established. Subsequently, the generation of bifurcation and stability of the model are dealt with. Delay-independent parameter criteria on bifurcation and stability are presented. Thirdly, a hybrid controller and an extended hybrid controller are designed to control the time of onset of bifurcation and stability domain of this model. The critical delay value is provided to display the bifurcation point. Last, software experiments are offered to support the acquired key outcomes. The established outcomes of this article are perfectly innovative and provide tremendous theoretical significance in balancing the oxygen density and the phytoplankton density in biology.
Green Extraction of Pectin from Sugar Beet Flakes and Its Application in Hydrogels and Cryogels
Authors: Dranca Florina , Mironeasa Silvia
Published: March 2024

Sugar beet flakes, a by-product of the sugar industry, were used as a source for pectin extraction that was performed using conventional citric acid extraction (CE) and two non-conventional extraction techniques—microwave-assisted extraction (MAE) and pulsed ultrasound-assisted extraction (PUAE). The influence of extraction conditions was studied for each technique based on pectin yield and galacturonic acid content, and spectroscopic, chromatographic and colorimetric methods were used for pectin characterization. Better results for pectin yield were achieved through CE (20.80%), while higher galacturonic acid content was measured in pectin extracted using PUAE (88.53 g/100 g). Pectin extracted using PUAE also presented a higher degree of methylation and acetylation. A significant increase in the molecular weight of pectin was observed for the PUAE process (7.40 × 105 g/mol) by comparison with conventional extraction (1.18 × 105 g/mol). Hydrogels and cryogels prepared with pectin from sugar beet flakes also showed differences in physicochemical parameters determined by the method of pectin extraction. Hydrogels had higher bulk density values irrespective of the pectin extraction method, and overall lower values of the textural parameters. Cryogels prepared with pectin from CE showed higher values of the textural parameters of hardness, adhesiveness, cohesiveness, gumminess and chewiness, while gels obtained with pectin from MAE and PUAE had higher thermal stability. The results of this study prove that sugar beet flakes can be considered a potential source for pectin production, and the extracted pectin is suitable for obtaining hydrogels and cryogels with physicochemical parameters comparable to the commercial citrus and apple pectin available on the market.
Genomic Prediction from Multi-Environment Trials of Wheat Breeding
Authors: García-Barrios Guillermo , Crespo-Herrera Leonardo , Cruz-Izquierdo Serafín , Vitale Paolo , Sandoval-Islas José Sergio , Gerard Guillermo Sebastián , Aguilar-Rincón Víctor Heber , Corona-Torres Tarsicio , Crossa José , Pacheco-Gil Rosa Angela
Published: March 2024

Genomic prediction relates a set of markers to variability in observed phenotypes of cultivars and allows for the prediction of phenotypes or breeding values of genotypes on unobserved individuals. Most genomic prediction approaches predict breeding values based solely on additive effects. However, the economic value of wheat lines is not only influenced by their additive component but also encompasses a non-additive part (e.g., additive × additive epistasis interaction). In this study, genomic prediction models were implemented in three target populations of environments (TPE) in South Asia. Four models that incorporate genotype × environment interaction (G × E) and genotype × genotype (GG) were tested: Factor Analytic (FA), FA with genomic relationship matrix (FA + G), FA with epistatic relationship matrix (FA + GG), and FA with both genomic and epistatic relationship matrices (FA + G + GG). Results show that the FA + G and FA + G + GG models displayed the best and a similar performance across all tests, leading us to infer that the FA + G model effectively captures certain epistatic effects. The wheat lines tested in sites in different TPE were predicted with different precisions depending on the cross-validation employed. In general, the best prediction accuracy was obtained when some lines were observed in some sites of particular TPEs and the worse genomic prediction was observed when wheat lines were never observed in any site of one TPE.
Cytogenomic Characterization of Transposable Elements and Satellite DNA in Passiflora L. Species
Authors: Silva Gonçalo Santos , Souza Margarete Magalhães , Pamponét Vanessa de Carvalho Cayres , Micheli Fabienne , Melo Cláusio Antônio Ferreira de , Oliveira Sárah Gomes de , Costa Eduardo Almeida
Published: March 2024

The species Passiflora alata, P. cincinnata, and P. edulis have great economic value due to the use of their fruits for human consumption. In this study, we compared the repetitive genome fractions of these three species. The compositions of the repetitive DNA of these three species’ genomes were analyzed using clustering and identification of the repetitive sequences with RepeatExplorer. It was found that repetitive DNA content represents 74.70%, 66.86%, and 62.24% of the genome of P. alata, P. edulis, and P. cincinnata, respectively. LTR Ty3/Gypsy retrotransposons represent the highest genome proportions in P. alata and P. edulis, while Ty1/Copia comprises the largest proportion of P. cincinnata genome. Chromosomal mapping by Fluorescent In Situ Hybridization (FISH) showed that LTR retrotransposons have a dispersed distribution along chromosomes. The subtelomeric region of chromosomes is where 145 bp satellite DNA is located, suggesting that these elements may play important roles in genome structure and organization in these species. In this work, we obtained the first global characterization of the composition of repetitive DNA in Passiflora, showing that an increase in genome size is related to an increase in repetitive DNA, which represents an important evolutionary route for these species.
Conceptual Model of Permafrost Degradation in an Inuit Archaeological Context (Dog Island, Labrador): A Geophysical Approach
Authors: Labrie Rachel , Bhiry Najat , Todisco Dominique , Finco Cécile , Couillet Armelle
Published: March 2024

Characterizing permafrost is crucial for understanding the fate of arctic and subarctic archaeological archives under climate change. The loss of bio-physical integrity of archaeological sites in northern regions is still poorly documented, even though discontinuous permafrost is particularly vulnerable to global warming. In this study, we documented the spatial distribution of the permafrost-supported Inuit archaeological site Oakes Bay 1 on Dog Island (Labrador, Canada) while employing a novel approach in northern geoarchaeology based on non-invasive geophysical methods. ERT and GPR were successfully used to estimate active layer thickness and image permafrost spatial variability and characteristics. The results made it possible to reconstruct a conceptual model of the current geocryological context of the subsurface in relation to the site topography, hydrology, and geomorphology. The peripherical walls of Inuit semi-subterranean sod houses were found to contain ice-rich permafrost, whereas their central depressions were identified as sources of vertical permafrost degradation. The geophysical investigations were used to classify the permafrost at Oakes Bay 1 as climate-driven, ecosystem-protected permafrost that cannot regenerate under current climate conditions. This work highlights how the permafrost at Oakes Bay 1 is currently affected by multi-point thermal degradation by both conduction and advection, which makes it highly sensitive to climate warming.
Effects of Advance Care Planning on End-of-Life Indicators for Nursing Home Residents—An Experimental Study with a Retrospective Chart Review
Authors: Lo Yu-Tai , Wang Jin-Jy , Yang Yi-Ching , Yu Chiu-Yen , Chang Chia-Ming , Yang Ya-Ping
Published: March 2024

Advance care planning (ACP) has the potential to improve the outcomes of end-of-life care for residents in nursing homes. The aim of this study was to determine whether an ACP program was beneficial for nursing home residents by assessing end-of-life indicators. An experimental study with a retrospective chart review was conducted. In total, 37 residents in the intervention group participated in an institutional advance care planning program for 1 year, and their chart data over 1 year were collected following the completion of the program; 33 residents in the control group had died within 1 year before the start date of program, and their chart data were reviewed retrospectively. Chi-square and t tests were used to examine four indicators of the quality of end-of-life care. Compared with the control group, the intervention group had a higher proportion of do-not-resuscitate directives, hospice care before death, and deaths in the nursing home, and fewer hospitalizations and deaths in an emergency department. ACP programs may improve the quality of end-of-life care for nursing home residents in Taiwan. Further research across different long-term care facilities is warranted.
Implementation of a Provincial Long COVID Care Pathway in Alberta, Canada: Provider Perceptions
Authors: Manhas Kiran Pohar , Horlick Sidney , Krysa Jacqueline , Kovacs Burns Katharina , Brehon Katelyn , Laur Celia , Papathanassoglou Elizabeth , Ho Chester
Published: March 2024

A novel, complex chronic condition emerged from the COVID-19 pandemic: long COVID. The persistent long COVID symptoms can be multisystem and varied. Effective long COVID management requires multidisciplinary, collaborative models of care, which continue to be developed and refined. Alberta’s provincial health system developed a novel long COVID pathway. We aimed to clarify the perspectives of multidisciplinary healthcare providers on the early implementation of the provincial long COVID pathway, particularly pathway acceptability, adoption, feasibility, and fidelity using Sandelowki’s qualitative description. Provider participants were recruited from eight early-user sites from across the care continuum. Sites represented primary care (n = 4), outpatient rehabilitation (n = 3), and COVID-19 specialty clinics (n = 2). Participants participated in structured or semi-structured virtual interviews (both group and 1:1 were available). Structured interviews sought to clarify context, processes, and pathway use; semi-structured interviews targeted provider perceptions of pathway implementation, including barriers and facilitators. Analysis was guided by Hsieh and Shannon as well as Sandelowski. Across the eight sites that participated, five structured interviews (n = 13 participants) and seven semi-structured interviews (n = 15 participants) were completed. Sites represented primary care (n = 4), outpatient rehabilitation (n = 3), and COVID-19 specialty clinics (n = 2). Qualitative content analysis was used on transcripts and field notes. Provider perceptions of the early implementation outcomes of the provincial long COVID pathway revealed three key themes: process perceptions; awareness of patient educational resources; and challenges of evolving knowledge.
Implementing Lived Experience Workshops in Regional Areas of British Columbia to Enhance Clinicians’ Confidence in Spinal Cord Injury Care: An Evaluation
Authors: Prins Hannah , Donia Scott , Rockall Shannon , Hektner James , Hawes Spring , Laskin James J. , Chernesky John , Noonan Vanessa K.
Published: March 2024

In British Columbia (BC), there are challenges accessing specialized spinal cord injury care and resources. This paper evaluated the impact of spinal cord injury health educational workshops delivered in regional communities that were informed by persons with lived experience. A community survey was conducted with 44 persons with lived experience in a BC region to identify priority SCI health-related topics. Twenty-five topics were ranked from 1–14, with bowel and bladder management ranked 1 and 4, sexual health ranked 5, and pressure injuries ranked 7. Clinical perspectives on the priorities were collected from 102 clinicians in the BC region, who independently ranked 14 of these SCI topics and considered the former 4 topics to be lower clinical priority (ranked 11–14). These priorities informed a series of SCI clinical education workshops held at healthcare facilities in three regional cities. The goals were to improve clinicians’ knowledge and confidence levels when managing spinal cord injury health and to facilitate person-centred care. Positive feedback demonstrated that educational workshops supported by lived experience perspectives effectively enhanced the clinicians’ understanding of spinal cord injury and their priorities. Future plans include engaging more administrators as part of this initiative and conducting workshops in other regions of BC.
Beyond the Clinic Walls: Examining Radiology Technicians’ Experiences in Home-Based Radiography
Authors: Lepri Graziano , Oddi Francesco , Gulino Rosario Alfio , Giansanti Daniele
Published: March 2024

In recent years, the landscape of diagnostic imaging has undergone a significant transformation with the emergence of home radiology, challenging the traditional paradigm. This shift, bringing diagnostic imaging directly to patients, has gained momentum and has been further accelerated by the global COVID-19 pandemic, highlighting the increasing importance and convenience of decentralized healthcare services. This study aims to offer a nuanced understanding of the attitudes and experiences influencing the integration of in-home radiography into contemporary healthcare practices. The research methodology involves a survey administered through Computer-Aided Web Interviewing (CAWI) tools, enabling real-time engagement with a diverse cohort of medical radiology technicians in the health domain. A second CAWI tool is submitted to experts to assess their feedback on the methodology. The survey explores key themes, including perceived advantages and challenges associated with domiciliary imaging, its impact on patient care, and the technological intricacies specific to conducting radiologic procedures outside the conventional clinical environment. Findings from a sample of 26 medical radiology technicians (drawn from a larger pool of 186 respondents) highlight a spectrum of opinions and constructive feedback. Enthusiasm is evident for the potential of domiciliary imaging to enhance patient convenience and provide a more patient-centric approach to healthcare. Simultaneously, this study suggests areas of intervention to improve the diffusion of home-based radiology. The methodology based on CAWI tools proves instrumental in the efficiency and depth of data collection, as evaluated by 16 experts from diverse professional backgrounds. The dynamic and responsive nature of this approach allows for a more allocated exploration of technicians’ opinions, contributing to a comprehensive understanding of the evolving landscape of medical imaging services. Emphasis is placed on the need for national and international initiatives in the field, supported by scientific societies, to further explore the evolving landscape of teleradiology and the integration of artificial intelligence in radiology. This study encourages expansion involving other key figures in this practice, including, naturally, medical radiologists, general practitioners, medical physicists, and other stakeholders.
The Genome-Wide Identification of the R2R3-MYB Gene Family in Chinese Flowering Cabbage and the Characterization of Its Response to Pectobacterium carotovorum Infection
Authors: Lei Shikang , Li Guangguang , Jiang Ding , Yuan Fanchong , Zhou Xianyu , Zheng Yansong , Zhang Hua , Cao Bihao
Published: March 2024

Chinese flowering cabbage is an important bolting stem vegetable widely grown in southern China, but severe losses caused by soft rot disease are very common in this crop. The MYB transcription factor (TF) family is the largest TF family in plants and plays diverse roles in response to stresses. However, the responses of MYB TFs to biotic stress in Chinese flowering cabbage have not been systematically studied. Herein, 255 R2R3-MYB genes were identified in the genome of Chinese flowering cabbage and classified into 29 subgroups based on phylogenetic comparisons with Arabidopsis thaliana. Gene duplication events involved 182 gene duplication pairs, and we found that two tandem duplication events involving R2R3-MYB genes in Chinese flowering cabbage may also affect gene family expansion. Transcriptome data analysis indicated that MYB TF genes are highly enriched in differentially expressed gene (DEG) sets. Combined with phylogenetic and transcriptome analysis, we identified 12 R2R3-MYB genes that potentially play a role in the response to soft rot stress. Our research provides a foundation for further research on the response of R2R3-MYB genes to soft rot stress in Chinese flowering cabbage.
“Death Is a Possibility for Those without Shelter”: A Thematic Analysis of News Coverage on Homelessness and the 2021 Heat Dome in Canada
Authors: Tetzlaff Emily J. , Mourad Farah , Goulet Nicholas , Gorman Melissa , Siblock Rachel , Kidd Sean A. , Bezgrebelna Mariya , Kenny Glen P.
Published: March 2024

Among the most vulnerable to the health-harming effects of heat are people experiencing homelessness. However, during the 2021 Heat Dome, the deadliest extreme heat event (EHE) recorded in Canada to date, people experiencing homelessness represented the smallest proportion of decedents (n = 3, 0.5%)—despite the impacted region (British Columbia) having some of the highest rates of homelessness in the country. Thus, we sought to explore the 2021 Heat Dome as a media-based case study to identify potential actions or targeted strategies that were initiated by community support agencies, individuals and groups, and communicated in the news during this EHE that may have aided in the protection of this group or helped minimize the mortality impacts. Using media articles collated for a more extensive investigation into the effects of the 2021 Heat Dome (n = 2909), we identified a subset which included content on people experiencing homelessness in Canada (n = 274, 9%). These articles were thematically analysed using NVivo. Three main themes were identified: (i) public warnings issued during the 2021 Heat Dome directly addressed people experiencing homelessness, (ii) community support services explicitly targeting this population were activated during the heat event, and (iii) challenges and barriers faced by people experiencing homelessness during extreme heat were communicated. These findings suggest that mass-media messaging and dedicated on-the-ground initiatives led by various organizations explicitly initiated to support individuals experiencing homelessness during the 2021 Heat Dome may have assisted in limiting the harmful impacts of the heat on this community.
Spatial Analysis of Exposure of Roads to Flooding and Its Implications for Mobility in Urban/Peri-Urban Accra
Authors: Yiran Gerald Albert Baeribameng , Oteng Ababio Martin , Allotey Albert Nii Moe , Kofie Richard Yao , Møller-Jensen Lasse
Published: March 2024

Climate change seriously threatens human systems, properties and livelihoods. Global projections suggest a continuous increase in the frequency and severity of weather events, with severe outcomes. Although the trends and impacts are highly variable depending on location, most studies tend to concentrate on either the urban or rural areas, with little focus on peri-urban areas. Yet, in Sub-Saharan Africa, peri-urban areas display unique characteristics: inadequate infrastructure, unplanned development, weak governance, and environmental degradation, all of which exacerbate flood impact and thus need academic attention. This study contributes to filling this gap by assessing the flood vulnerability of roads in peri-urban Accra and its implications for mobility. Based on the fieldwork, the study delineated and analysed potential zones within the research locations. The researchers calculated roads’ absolute and relative lengths, using a spatial overlay (intersection) of potentially flooded roads with the total road network within the grid cells of 500 m by 500 m. These measures were adopted and used as exposure measures. The findings revealed that over 80% of roads with lengths between 100 m and 500 m were exposed to floods. Some areas had higher exposure indices, with absolute road lengths ranging from 1.5 km to 3.2 km and relative road lengths between 0.8 and 1.0. There were significant variations in road exposure between and within neighbourhoods. Depending on the depth and duration of the floodwater, residents may be unable to access their homes or carry out their daily activities. In conclusion, this study highlights the differential vulnerability of peri-urban areas to road flooding and recommends targeted provision of flood-resilient infrastructure to promote sustainable development.
Cryptomphalus aspersa Egg Extract Protects against Human Stem Cell Stress-Induced Premature Senescence
Authors: Outskouni Zozo , Christodoulou Christina , Goutas Andreas , Kyriazis Ioannis D. , Paraskevopoulou Adamantini , Laliotis George P. , Matsakidou Anthia , Gogas Athanasios , Trachana Varvara
Published: March 2024

Cellular senescence is a tightly regulated pathophysiologic process and is caused by replicative exhaustion or external stressors. Since naturally derived bioactive compounds with anti-ageing properties have recently captured scientific interest, we analysed the anti-ageing and antioxidant efficacy of Cryptomphalus aspersa egg extract (CAEE). Its effects on stemness, wound-healing properties, antioxidant defense mechanisms, and DNA damage repair ability of Human Wharton’s jelly mesenchymal stem cells (WJ-MSCs) were analysed. Our results revealed that CAEE fortifies WJ-MSCs stemness, which possibly ameliorates their wound-healing ability. Additionally, we show that CAEE possesses a strong antioxidant capacity as demonstrated by the elevation of the levels of the basic antioxidant molecule, GSH, and the induction of the NRF2, a major antioxidant regulator. In addition, CAEE alleviated cells’ oxidative stress and therefore prevented stress-induced premature senescence (SIPS). Furthermore, we demonstrated that the prevention of SIPS could be mediated via the extract’s ability to induce autophagy, as indicated by the elevation of the protein levels of all basic autophagic molecules and the increase in formation of autophagolysosomes in CAEE-treated WJ-MSCs. Moreover, CAEE-treated cells exhibited decreased Caveolin-1 levels. We propose that Cryptomphalus aspersa egg extract comprises bioactive compounds that can demonstrate strong antioxidant/anti-ageing effects by regulating the Caveolin-1–autophagy–senescence molecular axis.
Stool Glycoproteomics Signatures of Pre-Cancerous Lesions and Colorectal Cancer
Authors: Soares Janine , Eiras Mariana , Ferreira Dylan , Santos Daniela A. R. , Relvas-Santos Marta , Santos Beatriz , Gonçalves Martina , Ferreira Eduardo , Vieira Renata , Afonso Luís Pedro , Santos Lúcio Lara , Dinis-Ribeiro Mário , Lima Luís , Ferreira José Alexandre
Published: March 2024

Colorectal cancer (CRC) screening relies primarily on stool analysis to identify occult blood. However, its sensitivity for detecting precancerous lesions is limited, requiring the development of new tools to improve CRC screening. Carcinogenesis involves significant alterations in mucosal epithelium glycocalyx that decisively contribute to disease progression. Building on this knowledge, we examined patient series comprehending premalignant lesions, colorectal tumors, and healthy controls for the T-antigen—a short-chain O-glycosylation of proteins considered a surrogate marker of malignancy in multiple solid cancers. We found the T-antigen in the secretions of dysplastic lesions as well as in cancer. In CRC, T-antigen expression was associated with the presence of distant metastases. In parallel, we analyzed a broad number of stools from individuals who underwent colonoscopy, which showed high T expressions in high-grade dysplasia and carcinomas. Employing mass spectrometry-based lectin-affinity enrichment, we identified a total of 262 proteins, 67% of which potentially exhibited altered glycosylation patterns associated with cancer and advanced pre-cancerous lesions. Also, we found that the stool (glyco)proteome of pre-cancerous lesions is enriched for protein species involved in key biological processes linked to humoral and innate immune responses. This study offers a thorough analysis of the stool glycoproteome, laying the groundwork for harnessing glycosylation alterations to improve non-invasive cancer detection.