bluuebunny/crossref_metadata_2025 huggingface dataset:
Influences of Land Policy on Urban Ecological Corridors Governance: A Case Study from Shanghai
Authors: Zhou Xiaoping , Shen Duanshuai , Gu Xiaokun
Published: August 2022

The analysis of land use change (LUC) characteristics and the impact of policies related to urban ecological space is required to improve spatial planning and to support decision making regarding green infrastructure (GI) investment. This study employed Geo-informatic Tupu analysis and Fluctuation Potential Tupu analysis methods to analyze the characteristics of LUC in an urban ecological corridor (EC). To help understand the influence of land use policy on GI governance and support the optimization of spatial planning, we proposed a situation–structure–implementation–outcome (SSIO) policy cascade analysis framework. SSIO takes “place” as its starting point, then couples the local policy with the governance structure to promote the sustainability of urban commons governance. The results show that the land use type within an EC in the city is mainly cultivated land. However, between 2009 and 2019, cultivated land, construction land, and facility agricultural land all showed a decreasing trend, while forest land and garden land types underwent increasing trends. The LUC Tupu unit highlights the transition from cultivated land to forest land. Forest land has the greatest increase in area and accounts for 52.34% of the area of increasing land use. Cultivated land shows the greatest decrease in area and accounts for 70.30% of the area of decreasing trends. Based on the local policy situation of the metropolis, a land policy governance mechanism can be constructed by the establishment of a governance structure with local government as the core, using land consolidation as the platform, taking ecological spatial planning and inefficient construction land reduction as typical policy tools, and experimentally integrating the concept of Nature-based Solutions (NbS). In general, these findings may be applicable to other rapidly urbanizing cities around the world that are developing complex land use policies for ecological space governance.
A Qualitative Exploration of the Functional, Social, and Emotional Impacts of the COVID-19 Pandemic on People Who Use Drugs
Authors: Kelly Erin L. , Reed Megan K. , Schoenauer Kathryn M. , Smith Kelsey , Scalia-Jackson Kristina , Kay Hill Sequoia , Li Erica , Weinstein Lara
Published: August 2022

Since 2020, people who use drugs (PWUD) experienced heightened risks related to drug supply disruptions, contamination, overdose, social isolation, and increased stress. This study explored how the lives of PWUD changed in Philadelphia over a one-year period. Using semi-structured interviews with 20 participants in a Housing First, low-barrier medication for opioid use (MOUD) program in Philadelphia, the effects of the first year of the COVID-19 pandemic on the daily lives, resources, functioning, substance use, and treatment of PWUD were explored. Interviews were analyzed using a combination of directed and conventional content analysis. Six overarching themes emerged during data analysis: (1) response to the pandemic; (2) access to MOUD and support services; (3) substance use; (4) impacts on mental health, physical health, and daily functioning; (5) social network impacts; and (6) fulfillment of basic needs. Participants reported disruptions in every domain of life, challenges meeting their basic needs, and elevated risk for adverse events. MOUD service providers offset some risks and provided material supports, treatment, social interaction, and emotional support. These results highlight how there were significant disruptions to the lives of PWUD during the first year of the COVID-19 pandemic and identified critical areas for future intervention and policies.
Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine
Authors: Kapczuk Patrycja , Kosik-Bogacka Danuta , Kupnicka Patrycja , Kopytko Patrycja , Tarnowski Maciej , Kolasa Agnieszka , Chlubek Dariusz , Baranowska-Bosiacka Irena
Published: August 2022

The rat tapeworm Hymenolepis diminuta has been shown to cause alterations in gastrointestinal tissues. Since hymenolepiasis induces a number of reactions in the host, it is reasonable to assume that it may also be involved in the mechanisms of apoptosis in the intestines. Individual research tasks included an examination of the effect of H. diminuta infection on; (i) the cellular localization of the expression of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2, as well as caspase-3 and caspase-9, and (ii) the effects of the infection on the expression of Bcl-2, Bax, Cas-3 and Cas-9, at the mRNA and protein levels. Molecular tests (including mRNA (qRT PCR) and the protein (Western blot) expression of Bax, Bcl-2, and caspases-3, -9) and immunohistochemical tests were performed during the experiment. They showed that H. diminuta infection activates the intrinsic apoptosis pathway in the small and large intestine of the host. H. diminuta infection triggered the apoptosis via the activation of the caspase cascade, including Cas-3 and Cas-9. Hymenolepiasis enhanced apoptosis in the small and large intestine of the host by increasing the expression of the pro-apoptotic gene and protein Bax and by decreasing the expression of the anti-apoptotic gene and protein Bcl-2.
Shallow Failure of Weak Slopes in Bayan Obo West Mine
Authors: Wang Wencai , Yan Yongfu , Qu Yue , Wang Pengfei
Published: August 2022

The slope stability of large open-pit mines has always been a concern and the analysis of large-scale slope landslides is a focus. However, shallow failure in soft rock slopes also has a serious impact on safe production. The northern slope of Baiyunebo West Mine has many shallow landslides in the final slope, resulting in damage of the maintenance channel of the belt transportation system, which has a serious impact on the safety of production. In order to reduce the shallow failure in weak rock slope, it is necessary to analyze the behavior and characteristics of shallow failure in weak rock. Firstly, the mechanical parameters of the intact rock were obtained by using the exploration data; secondly, through the analysis of blasting-damage range, the distribution characteristics of fractures after the failure of weak rock were obtained. Finally, through theoretical analysis, numerical simulation, surface displacement monitoring and on-site shallow-failure case analysis, the deformation and characteristics of shallow failure of weak rock slope in West Mine were obtained. It was found that the mechanical parameters of rock mass strength on the surface of weak rock slope and the original rock were quite different after mining disturbance. The mode of failure of shallow weak rock slope in the West Mine was creep-cracking; the numerical modelling analysis was carried out by using the assignment method of shallow lithology weakening and gradual change, which is more in line with the deformation characteristics of weak rock slope in West Mine. The lower deformation of the soft rock slope in West Mine is 3–5 times that of the upper deformation. The research results are helpful to understand the influence of blasting on the stability of soft rock slope. At present, West Mine has started to adjust blasting parameters according to the research results, so as to reduce the excessive damage of blasting to rock mass, so the stability of the slope is improved.
Gaps and Directions in Addressing Non-Communicable and Chronic Diseases in China: A Policy Analysis
Authors: Jiang Yingying , Guo Haijun , Zhang Weiwei , Gan Connie C. R. , Mao Fan , Zhou Maigeng , Phung Hai , Phung Dung , Dong Jianqun , Chu Cordia
Published: August 2022

Objective: Non-communicable and chronic diseases (NCDs) have become a public health problem that seriously threatens the population’s health, especially in rapidly industrialized countries. Given the complexity of managing NCDs, there is growing evidence that interventions embedding or incorporating health promotion strategies can help reduce the disease burden of NCDs. This review aims to identify and map existing control and prevention policies for NCDs in China and identify gaps or opportunities for policy modifications and development, to transfer evidence-based guidelines into empirical public health intervention practices and research. Design: A review was conducted to evaluate the policy documents to manage NCDs in China. Keywords “chronic disease”, “health”, and “policy” were used to search documents published on the Chinese official national websites. Nvivo 12.0 was used to conduct a content analysis of the policy documents. Results: Fifty-six NCD prevention policies were retrieved from the search, and ten documents that incorporated the health promotion component were analyzed. The Healthy China 2030 Plan and Recommendations on Implementing the Healthy China Action are the core health policies of China’s Central Government. These, coupled with three nationwide community-based projects, have provided a foundational transformation platform for health promotion implementation. Conclusion: This review revealed the Chinese Government’s determination and commitment toward the prevention and control of NCDs and the promotion public health. Additional efforts and a focus on accelerating policy transformation and strengthening policy evaluation are required to achieve this commitment.
Problematic Social Media Use and Cyber Aggression in Italian Adolescents: The Remarkable Role of Social Support
Authors: Borraccino Alberto , Marengo Noemi , Dalmasso Paola , Marino Claudia , Ciardullo Silvia , Nardone Paola , Lemma Patrizia ,   The 2018 HBSC-Italia Group
Published: August 2022

The recent increase in electronic and social media use among young people has highlighted the importance of focusing on problematic social media use (PSMU) and the concurrent phenomenon of cyber aggression, as well as the role of social support. As part of the 2018 Health Behavior in School-aged Children study in Italy, this study aims to explore the role of family, peer, and teacher support in the association between cyberbullying and PSMU. Methods: Data were collected from 4183 school classes in Italy for a total of 58,976 adolescents aged 11, 13, and 15 years. The prevalence of cyber aggression (both cybervictimization and perpetration) and PSMU was estimated across subgroups of different age, gender, and geographical residence. A set of multivariable logistic regressions was then used to investigate the association between cyberbullying and PSMU, considering the effect of social support. Results: Cybervictimization was more frequent among girls than in boys. PSMU was higher in 11-year-old boys and 13-year-old girls; 8.3% and 12.7%, respectively. Social support was highest in 11-year-olds, for both sexes, and then decreased with increasing age. The risk of cyberbullying, both suffered and perpetrated, was higher in girls and in the presence of PSMU. Social support showed to be highly protective against PSMU and cyberbullying in all ages and both genders. Conclusion: Although cyber aggression is less represented in Italy than in other European countries, it is likely to increase. PSMU appears to be an important contributor to the risk of cyber aggression; however, social support has been shown to be capable of reducing the risk of both phenomena. Public health policies fostering familiar and school support can help protect adolescents’ mental health, reducing the risk of problematic media use and cyberbullying.
Quality of Life and Mental Health Benefits of Public Participation in Forest Conservation Activities in Urban Areas
Authors: Joung Dawou , Park Bum-Jin , Kang Shinkwang
Published: August 2022

The purpose of this study is to investigate the effect of forest conservation activities on the physical and psychological wellbeing of participants. The experiment was conducted in a forest near an urban area and involved 61 participants (average age: 22.5 ± 1.8). The participants selected one of three activities (pruning, stacking cut branches, and removing vines) in the forest conservation program. The effects of these activities on the musculoskeletal system were assessed using the Ovako Working Posture Assessment System (OWAS); the physical intensity of the activities was evaluated using heart rate data. The psychological evaluation measurement indexes used the Positive and Negative Affect Schedule, Rosenberg Self-Esteem scale, World Health Organization Quality of Life assessment instrument, and the Perceived Restorativeness Scale. As a result of the OWAS assessment, forest conservation activities were found to be action categories 1 and 2, which were less burdensome to the musculoskeletal system. All forestry activities were found to be light levels of physical intensity. Psychological evaluation of the participants revealed that positive emotions such as self-esteem, quality of life, and perceived restorativeness increased significantly, whereas negative emotions decreased significantly. This forest conservation program, that involved low-intensity activities which were less burdensome to the musculoskeletal system, had positive physical and psychological effects on the local residents who participated.
Impact of Education for Sustainable Development on Cognition, Emotion, and Behavior in Protected Areas
Authors: Ding Yan , Zhao Minyan , Li Zehong , Xia Bing , Atutova Zhanna , Kobylkin Dmitry
Published: August 2022

Education for sustainable development (ESD) of protected areas is proposed to deal with global climate change and biodiversity conversation. It focuses on the “quality education” and “protection” of the United Nations’ sustainable development goals (UN SDGs), not only taking protected areas as the education place, but also as the theme and content of education. Based on cognitive-behavior theory and social emotional learning theory, this study constructs a “cognitive–emotion–behavior” dimension framework of ESD in protected areas, selecting Potatso National Park in Yunnan as a case study. Based on 529 valid visitor questionnaires, this study uses structural equation modeling to verify theoretical hypotheses, and analyzes the impact of ESD in protected areas on public cognition, emotion, and behavior. The results show that: (1) Cognitive and emotional factors jointly drive the behavioral intentions of ESD in protected areas, and social-emotional factors are slightly higher than cognitive factors; (2) Environmental knowledge, personal norms, nature connectedness, and places attachment positively affects behavioral intentions; (3) Indigenous knowledge has an impact on behavioral intentions through emotional mediation, and personal norms have an impact on behavioral intentions through direct effects; (4) Gender and visit frequency are important moderating variables in the ESD of protected areas. These conclusions provide the following suggestions for further development of ESD. First, by forming environment-friendly social norms and focusing on the mining and presentation of indigenous knowledge, the behavioral intention can also be enhanced to a certain extent; second, improving people’s emotion can also promote people’s behavioral intention, especially referring to optimizing nature connectedness, strengthening place attachment, and creating emotional connections; Third, specific groups of people should be taught specifically, and improve the supporting services of ESD.
A Randomized Clinical Trial to Assess the Efficacy of a Psychological Treatment to Quit Smoking Assisted with an App: Study Protocol
Authors: López-Durán Ana , Becoña Elisardo , Senra Carmen , Suárez-Castro Daniel , Barroso-Hurtado María , Martínez-Vispo Carmela
Published: August 2022

Numerous studies have shown the efficacy of smoking cessation interventions. However, some challenges, such as relapse rates, remain. The availability of information technologies (ICTs) offers promising opportunities to address such challenges. The aim of this paper is to describe the protocol followed to assess the efficacy of a face-to-face cognitive–behavioral intervention for smoking cessation using a smartphone application as a complement, compared with a control group. A single blind, two-arm, randomized controlled trial is proposed (NCT04765813). The participants will be smokers over 18 years old, who smoke at least eight cigarettes per day. Participants will be randomized to one of two conditions, using a 1:1 allocation ratio: (1) cognitive–behavioral smoking cessation treatment along with an App with active therapeutic components (SinHumo App); or (2) cognitive–behavioral treatment along with the use of a control App (without active components). The experimental App will be used during the eight treatment sessions and for 12 months after the end of treatment. The primary outcome measures will be 7-days point-prevalence abstinence at 12-months follow-up. We expect the experimental App to obtain higher abstinence rates at the end of treatment and at one-year post-treatment follow-ups and lower relapse rates, compared to the control App.
Why Is Health Care for Children with Down Syndrome So Crucial from the First Days of Life? A Retrospective Cohort Study Emphasized Transient Abnormal Myelopoiesis (TAM) Syndrome at Three Centers
Authors: Telman Gabriela , Sosnowska-Sienkiewicz Patrycja , Strauss Ewa , Mazela Jan , Mańkowski Przemysław , Januszkiewicz-Lewandowska Danuta
Published: August 2022

Down syndrome (DS) is a common genetic disorder and is associated with an increased likelihood of many diseases, including defects of the heart, genitourinary system, gastrointestinal tract, and oncological diseases. The aim of this study was to analyze medical problems occurring in newborns with DS and to create a basic diagnostic and therapeutic algorithm intended primarily for neonatologists, pediatricians, family physicians, and physicians of other specialties caring for children with DS. Over a 5-year period, the medical records of 161 neonates with Down syndrome from four neonatology departments in Poznan, Poland, were examined. After applying exclusion criteria, 111 patients were analyzed. Data obtained from medical history included sex, week of gestation, birth weight, APGAR score, clinical symptoms, peripheral blood count with smear, and clinical features such as jaundice, hemorrhagic diathesis, ascites, hepato- or splenomegaly, pericardial or pleural effusion, respiratory failure, and other rare transient signs of abnormal myelopoiesis: fetal edema, hepatic fibrosis, renal failure, and rush. In the study group, 8% of children with Down syndrome were diagnosed with a heart and 1.8% with a genitourinary defect. Transient abnormal myelopoiesis syndrome (Transient abnormal myelopoiesis (TAM)) was found in 10% of newborns with DS. A blood count with blood smear, cardiology consultation with echocardiography, and an abdominal ultrasound should be performed in the first few days after birth in all newborns with Down syndrome. If this is not possible and the child’s condition is stable, these tests can be performed within 2–3 months after birth.
The Influence of Maturity Status on Dynamic Balance Following 6 Weeks of Eccentric Hamstring Training in Youth Male Handball Players
Authors: Nebigh Ammar , Hammami Raouf , Kasmi Sofien , Rebai Haithem , Drury Benjamin , Chtara Mokhtar , van den Tillaar Roland
Published: August 2022

Information about when to apply an appropriate eccentric hamstring strength training stimulus during long-term athlete development is essential for effective programming and improving balance performance. This study examined the effects of six-week eccentric hamstring training on dynamic balance performance in youth handball players of different maturity statuses (pre- or post-peak height velocity (PHV)). A randomized controlled design with 45 young male handball players (14.6 ± 0.3 years) from a local national handball club were randomly allocated to a 6 week, twice weekly eccentric hamstring training in two experimental groups: a pre-PHV (maturation offset: −2.13 ± 0.63, n = 10) and post-PHV (maturation offset: 0.79 ± 0.34, n = 12) group and two control groups: maturation offset: −2.09 ± 0.61, n = 10 and maturation offset: 0.55 ± 0.67, n = 13. Dynamic balance performance was evaluated by using the composite score during the lower quarter Y-balance test from pre- and post-intervention. A significant effect on balance scores was found from pre to post (F = 11.4; p = 0.002; η2p = 0.22), intervention (F = 5.4; p = 0.025; η2p = 0.12), and maturation (F = 369; p < 0.001; η2p = 0.9), but no significant interaction effects were found (F ≥ 3.3; p ≥ 0.077; η2p ≤ 0.07). Post hoc analysis revealed that the post-PHV group had a higher score than the pre-PHV group. Furthermore, that dynamic balance increased in the post-PHV group after intervention much more in the control post-PHV group. It was concluded that somatic maturation influences dynamic balance performance and that eccentric hamstring training results in greater improvement in balance performance in young male handball players for the post-pubertal group.
Markowitz Mean-Variance Portfolio Optimization with Predictive Stock Selection Using Machine Learning
Authors: Chaweewanchon Apichat , Chaysiri Rujira
Published: August 2022

With the advances in time-series prediction, several recent developments in machine learning have shown that integrating prediction methods into portfolio selection is a great opportunity. In this paper, we propose a novel approach to portfolio formation strategy based on a hybrid machine learning model that combines convolutional neural network (CNN) and bidirectional long short-term memory (BiLSTM) with robust input features obtained from Huber’s location for stock prediction and the Markowitz mean-variance (MV) model for optimal portfolio construction. Specifically, this study first applies a prediction method for stock preselection to ensure high-quality stock inputs for portfolio formation. Then, the predicted results are integrated into the MV model. To comprehensively demonstrate the superiority of the proposed model, we used two portfolio models, the MV model and the equal-weight portfolio (1/N) model, with LSTM, BiLSTM, and CNN-BiLSTM, and employed them as benchmarks. Between January 2015 and December 2020, historical data from the Stock Exchange of Thailand 50 Index (SET50) were collected for the study. The experiment shows that integrating preselection of stocks can improve MV performance, and the results of the proposed method show that they outperform comparison models in terms of Sharpe ratio, mean return, and risk.
Epicardial Adipose Tissue in Patients with Coronary Artery Disease: A Meta-Analysis
Authors: Wang Qingpeng , Chi Jiangyang , Wang Chen , Yang Yun , Tian Rui , Chen Xinzhong
Published: August 2022

Objective: The aim of this study is to assess the association between epicardial adipose tissue (EAT) and coronary artery disease (CAD) via meta−analysis. Methods: Specific searches of online databases from January 2000 to May 2022 were conducted. All observational studies evaluating the association between EAT and CAD in PubMed, Web of Science, and the Cochrane Library databases were screened. A meta-analysis was conducted following the Preferred Reporting Items for Systematic Reviews and Meta−Analyses guidelines (PRISMA). In total, 21 studies encompassing 4975 subjects met the inclusion criteria, including 2377 diagnosed and assigned as the CAD group, while the other 2598 were assigned as the non−CAD group. Subjects in the CAD group were further divided into the severe stenosis group (stenosis ≥ 50%, n = 846) and the mild/moderate stenosis group (stenosis < 50%, n = 577). Results: Both the volume and thickness of EAT in the CAD group were larger compared to the non−CAD group (p < 0.00001). In a subgroup analysis within the CAD group, the severe stenosis group had a larger volume and thickness with respect to EAT when compared to the mild/moderate group (p < 0.001). Conclusions: The enlargement of EAT presented in CAD patients with an association with CAD severity. Although limited by different CAD types and measuring methods for EAT, as well as a smaller sample size, our results suggest that EAT is a novel predictor and a potential therapeutic target for CAD.
Doxycycline Attenuates Doxorubicin-Induced Cardiotoxicity by Improving Myocardial Energy Metabolism in Rats
Authors: Dantas Danielle , Pereira Amanda Gomes , Fujimori Anderson Seiji Soares , Ribeiro Ana Paula Dantas , de Almeida Silva Carol Cristina Vágula , Monte Marina Gaiato , Corrêa Camila Renata , Fernandes Ana Angélica , Bazan Silmeia Garcia Zanati , Azevedo Paula Schmidt , Minicucci Marcos Ferreira , de Paiva Sergio Alberto Rupp , Zornoff Leonardo Antônio Mamede , Polegato Bertha Furlan
Published: August 2022

Aim: Evaluate the influence of doxycycline, an anti-inflammatory and matrix metalloproteinase (MMP) inhibitor, on the attenuation of chronic doxorubicin-induced cardiotoxicity in rats. Methods: We allocated male Wistar rats into four groups: control (C), doxorubicin (D), doxycycline (inhibitor of MMP, IM), and Dox + doxycycline (DIM). Groups IM and DIM received doxycycline (5 mg/kg, IP) once a week for 4 weeks. In addition, 48 h after every doxycycline injection, groups D and DIM received Dox (5 mg/kg, IP). We performed echocardiogram and evaluated TIMP-4 and collagen I protein expression, MMP-2 activity, and oxidative stress and myocardial metabolism. Results: Doxorubicin promotes left atrium (LA) and left ventricle (LV) dilatation and decreases in LV fractional shortening, which was improved by doxycycline. Moreover, doxycycline attenuated the LV cardiomyocyte hypertrophy and collagen type I expression. Doxorubicin increased phosphofructokinase and decreased beta-hydroxyacyl Co-A dehydrogenase, pyruvate dehydrogenase, citrate synthase, and ATP synthase activity, which was partially attenuated by doxycycline. Lastly, doxycycline improved antioxidant enzyme activity in the DIM group. Conclusion: Doxorubicin increases oxidative stress and promotes changes in myocardial energy metabolism, accompanied by structural and functional changes. Doxycycline attenuated the doxorubicin-induced cardiotoxicity, at least in part, through changes in myocardial energy metabolism.
Newer and Emerging LDL-C Lowering Agents and Implications for ASCVD Residual Risk
Authors: Rikhi Rishi , Shapiro Michael D.
Published: August 2022

Multiple lines of evidence demonstrate that low-density lipoprotein-cholesterol causes atherosclerotic cardiovascular disease. Thus, targeting and lowering low-density lipoprotein-cholesterol is the principal strategy to reduce cardiovascular disease risk in primary and secondary prevention. Statin therapy is the foundation of lipid-lowering treatment, but adherence rates are low, and many individuals do not attain target low-density lipoprotein-cholesterol values. Additionally, most statin-treated patients are still at considerable atherosclerotic cardiovascular disease risk, emphasizing the need for more aggressive low-density lipoprotein-cholesterol-lowering therapies. The purpose of this review is to discuss new and emerging approaches to further lower low-density lipoprotein-cholesterol, including inhibition of ATP-citrate lyase, proprotein convertase subtilisin-kexin type 9, angiopoietin-related protein 3, and cholesteryl ester transfer protein.
Complete Right Bundle Branch Block as a Predictor of Cardiovascular Events in Type 2 Diabetes
Authors: Ono Katsuhiro , Uchimoto Sadahiko , Miyazaki Masamune , Honda Natsuki , Mori Katsuhito , Morioka Tomoaki , Imai Takumi , Shoji Tetsuo , Emoto Masanori
Published: August 2022

Complete right bundle branch block (CRBBB) is generally regarded as a clinically insignificant abnormality on an electrocardiogram, although its predictive value for cardiovascular events in type 2 diabetes mellitus (T2DM) is unknown. We examined the association of CRBBB with cardiovascular events during a 6-year follow-up in a single-center cohort study. The Fine–Gray model was used to analyze the independent association between CRBBB and composite cardiovascular events including cardiovascular death, nonfatal myocardial infarction, nonfatal stroke, and hospitalization for heart failure during follow up. We analyzed the data of 370 T2DM patients including 62 patients with pre-existing heart disease. CRBBB was found in 34 patients (9.2%). The composite cardiovascular outcome was recorded in 32 patients. When analyzed with the Fine–Gray model with inverse probability of treatment weighting, CRBBB was significantly associated with a higher risk of the cardiovascular outcome (hazard ratio, 2.55; 95% confidence interval, 1.04 to 6.26; p = 0.041). This association remained significant even after further adjustment for each of the potential confounders. This study suggested that CRBBB was an independent predictor of cardiovascular events in T2DM. Further studies with a larger sample size are warranted.
Middle Cerebral Artery M2 Thrombectomy: Safety and Technical Considerations in the German Stroke Registry (GSR)
Authors: Herzberg Moriz , Dorn Franziska , Trumm Christoph , Kellert Lars , Tiedt Steffen , Feil Katharina , Küpper Clemens , Wollenweber Frank , Liebig Thomas , Zimmermann Hanna
Published: August 2022

There is ongoing debate concerning the safety and efficacy of various mechanical thrombectomy (MT) approaches for M2 occlusions. We compared these for MT in M2 versus M1 occlusions. Subgroup analyses of different technical approaches within the M2 MT cohort were also performed. Patients were included from the German Stroke Registry (GSR), a multicenter registry of consecutive MT patients. Primary outcomes were reperfusion success events. Secondary outcomes were early clinical improvement (improvement in NIHSS score > 4) and independent survival at 90 days (mRS 0–2). Out of 3804 patients, 2689 presented with M1 (71%) and 1115 with isolated M2 occlusions (29%). The mean age was 76 (CI 65–82) and 77 (CI 66–83) years, respectively. Except for baseline NIHSS (15 (CI 10–18) vs. 11 (CI 6–16), p < 0.001) and ASPECTS (9 (CI 7–10) vs. 9 (CI 8–10, p < 0.001), baseline demographics were balanced. Apart from a more frequent use of dedicated small vessel stent retrievers (svSR) in M2 (17.4% vs. 3.0; p < 0.001), intraprocedural aspects were balanced. There was no difference in ICH at 24 h (11%; p = 1.0), adverse events (14.4% vs. 18.1%; p = 0.63), clinical improvement (62.5% vs. 61.4 %; p = 0.57), mortality (26.9% vs. 22.9%; p = 0.23). In M2 MT, conventional stent retriever (cSR) achieved higher rates of mTICI3 (54.0% vs. 37.7–42.0%; p < 0.001), requiring more MT-maneuvers (7, CI 2–8) vs. 2 (CI 2–7)/(CI 2–2); p < 0.001) and without impact on efficacy and outcome. Real-life MT in M2 can be performed with equal safety and efficacy as in M1 occlusions. Different recanalization techniques including the use of svSR did not result in significant differences regarding safety, efficacy and outcome.
Bone Turnover Markers and Bone Mineral Density in Children with Hypophosphatemic Rickets
Authors: Michałus Izabela , Łupińska Anna , Woch Izabela , Wieczorek-Szukała Katarzyna , Chlebna-Sokół Danuta , Lewiński Andrzej
Published: August 2022

Hypophosphatemic rickets is a rare disease that results in bone deformities. However, little is known about bone turnover and bone mass disorders in this disease. This retrospective study included 12 children aged 1–16 years diagnosed with hypophosphatemic rickets. Parameters of calcium-phosphate metabolism and bone turnover markers were analysed. Bone mineral density was assessed with the use of dual-energy X-ray absorptiometry, and indices of quantitative ultrasound examination of tibiae and radial bones were analysed. In the majority of patients, hypophosphatemia and hyperphosphaturia were present. The assessed bone turnover markers showed increased bone formation. Increased pyridinoline levels were found in 5 out of 12 patients. Bone mineral density was decreased only in one patient. Decreased values of quantitative ultrasound examination were observed in all the analysed patients. Conclusions: (1) Bone metabolism disturbances, reflected in the increased values of bone turnover markers and worse bone quality, were found in the group of patients with hypophosphatemic rickets. (2) It is crucial to determine bone turnover markers, dual-energy X-ray absorptiometry findings and indices of quantitative ultrasound examination in order to monitor progress of the disease, as well as treatment effects.
Long-Term Outcomes Associated with NAFLD, ASCVD, and All-Cause Mortality of Patients with Metabolic Syndrome
Authors: Jitrukthai Suchanart , Kositamongkol Chayanis , Boonchai Punyisa , Mepramoon Euarat , Ariyakunaphan Pinyapat , Nimitpunya Pongpol , Srivanichakorn Weerachai , Chaisathaphol Thanet , Washirasaksiri Chaiwat , Auesomwang Chonticha , Sitasuwan Tullaya , Tinmanee Rungsima , Sayabovorn Naruemit , Charatcharoenwitthaya Phunchai , Phisalprapa Pochamana
Published: August 2022

Metabolic syndrome (MetS) patients are at higher risk for nonalcoholic fatty liver disease (NAFLD), atherosclerotic cardiovascular diseases (ASCVD), and death. Given a lack of longitudinal data on patients with MetS in Southeast Asia, this study investigated the incidence of NAFLD and ASCVD and the all-cause mortality rate during a 10-year follow-up of Thai patients with MetS. Retrospective data were collected on 496 MetS patients with ultrasonography or transient elastography results. The patients had been followed up continuously by a university hospital between October 2011 and November 2021, and their mean age was 61.0 ± 10.9 years. Patients with secondary causes of hepatic steatosis were excluded. Cox proportional hazards regression models with time-varying covariates were adopted. During the 10-year follow-up, 17 patients (11.2%) developed NAFLD, and 27 (6.4%) developed ASCVD. The NAFLD and ASCVD incidence rates were 21.7 and 10.9 events per 1000 person years, respectively. The mortality rate was 14.2 deaths per 1000 person years. The prevalence of hypertension, dyslipidemia, ASCVD, NAFLD, advanced fibrosis, and cirrhosis at baseline was significantly higher in the nonsurvival group. The NAFLD incidence and mortality rate of patients with MetS were lower than those in previous studies. Intensive, holistic, and continuous care should be considered for better outcomes.
Anti-Hypertensive Activity of Some Selected Unani Formulations: An Evidence-Based Approach for Verification of Traditional Unani Claims Using LC-MS/MS for the Evaluation of Clinically Relevant Blood Parameters in Laboratory Rats
Authors: Shaharyar Md. Adil , Bhowmik Rudranil , Afzal Obaid , Altamimi Abdulmalik S. A. , Alzarea Sami I. , Almalki Waleed Hassan , Ali Sk Zeeshan , Mandal Pallab , Mandal Avishek , Ayoob Mohd , Kazmi Imran , Karmakar Sanmoy
Published: August 2022

Background: Systemic arterial hypertension, which is associated with an increased risk of cardiovascular disease(CVD), is the most significant modifiable risk factor for mortality and morbidity worldwide. WHO has recognized Unanipathy as an alternate system of medicine. The aim of the present study is to investigate the anti-hypertensive activity of some selected unani formulations using L-NAME model. Method: Group I or hypertensive control group: L-NAME administered for 7 days and left for the next 7 days; Group II or KASgroup: L-NAME administered (i.p) for 7 days and L-NAME + KAS (1000 mg/kg b.w) for the next 7 days; Group III or DMM group: L-NAME administered (i.p) for 7 days and L-NAME + DMM (2000 mg/kg b.w) for the next 7 days; Group IV or MSR group: L-NAME administered (i.p) for 7 days and L-NAME + MSR (300 mg/kg b.w) for the next 7 days; Group V or HJ group: L-NAME administered (i.p) for 7 days and L-NAME + HJ (113 mg/kg b.w) for the next 7 days; Group VI or KGS group: L-NAME administered (i.p) for 7 days and L-NAME +KGS (2000 mg/kg b.w) for the next 7 days. Non-invasive systolic blood pressure and RR-interval (ECG) was measured. Plasma was investigated forsodium, potassium, nitrite, ANP, adrenaline, noradrenaline and aldosterone on day 0, 7 and 14 using LC-MS/MS. Result: Treatment showed a non-significant lowreduction in SBP (systolic blood pressure) of KAS, MSR and HJ while that of DMM was quite significant (p < 0.05), but in the case of KGS, SBP increased. DMM on day 14 significantly (p < 0.05) reduced plasma nitrite while no significant plasma Na+ was noted. In the case of both DMM and KGS, potassium increased significantly (p < 0.05) on day 14. No significant changes in plasma ANP and aldosterone was observed against DMM and KGS while blood levels of adrenaline and noradrenaline significantly (p < 0.05) changed. No significant change in body weight was found. Conclusions: L-NAME KAS, MSR and HJ showed no change in SBP while DMM showed a significant reduction in SBP with decreased plasma nitrite. Probably, DMM may have anti-hypertensive activity mediated through NO inhibition while KGS may involve central sympathomimetic action.
microRNAs in Subarachnoid Hemorrhage (Review of Literature)
Authors: Makowska Marianna , Smolarz Beata , Romanowicz Hanna
Published: August 2022

Recently, many studies have shown that microRNAs (miRNAs) in extracellular bioliquids are strongly associated with subarachnoid hemorrhage (SAH) and its complications. The article presents issues related to the occurrence of subarachnoid hemorrhage (epidemiology, symptoms, differential diagnosis, examination, and treatment of the patient) and a review of current research on the correlation between miRNAs and the complications of SAH. The potential use of miRNAs as biomarkers in the treatment of SAH is presented.
Influence of Post-Processing Conditions on the Microstructure, Static, and Fatigue Resistance of Laser Powder Bed Fused Ti-6Al-4V Components
Authors: Jimenez Erika Herrera , Kreitcberg Alena , Moquin Etienne , Brailovski Vladimir
Published: August 2022

The microstructure, static, and fatigue mechanical properties of laser powder bed fused (LPBF) Ti-6Al-4V components subjected to three different post-processing treatments (PTs) are compared. The first treatment includes stress relief (SR) and beta-phase annealing (BA) heat treatments, the second one includes SR, beta-solution (BST) and over aging (OA) heat treatments, and the third procedure is a combination of hot isostatic pressing (HIP) and BST + OA. It was demonstrated that the three PTs led to the decomposition of α’ martensite inherited from the LPBF process and the formation of variable α + β structures. The SR + BA treatment forms a basket weave structure having an average α lamellae width of ~3 µm and surrounded by ~1 µm-sized zones of segregated β phase (4.6–5.2% β phase content) and globalized α phase (~10 µm in size) inside prior columnar β grains (~100 µm in width). The SR + BST + OA treatment forms semi-equiaxed α grains (~300 µm) containing colonies (~50 µm) of parallel-oriented α plates (~6 µm), and β phase (5.8–7.5%) in the interplate spacing. The HIP + BST + OA treatment leads to the formation of large grains (~500 µm) with both basket weave and colony (~40 µm) α structures containing α plates (1.1–4.2 µm) and β phase (5–7.1%). To compare the impact of these PTs on the mechanical properties of LPBF components, they were subjected to static and fatigue tensile testing at room temperature. The best combination of mechanical properties (yield strength ~920 MPa, ultimate strength ~1000 MPa, elongation to break ~22.5%, and fatigue strength ~600 MPa, 107 cycles) was obtained in the case of SR + BA specimens. These results demonstrate that an adequate thermal treatment, such as SR + BA, of the LPBF Ti64 components, could be a valuable and less expensive alternative to the established HIP + BST + OA treatment procedure when fatigue life is the main concern.
Numerical Analysis of an Overtopping Wave Energy Converter Subjected to the Incidence of Irregular and Regular Waves from Realistic Sea States
Authors: Hubner Ricardo G. , Fragassa Cristiano , Paiva Maycon da S. , Oleinik Phelype H. , Gomes Mateus das N. , Rocha Luiz A. O. , Santos Elizaldo D. dos , Machado Bianca N. , Isoldi Liércio A.
Published: August 2022

The present study aims to evaluate the difference in the fluid-dynamic behavior of an overtopping wave energy converter under the incidence of irregular waves based on a realistic sea state when compared to the incidence of regular waves, representative of this sea state. Thus, the sea data of three regions from the Rio Grande do Sul coast, Brazil, were considered. Fluent software was employed for the computational modeling, which is based on the finite volume method (FVM). The numerical generation of waves occurred through the imposition of the velocity boundary conditions using transient discrete values through the WaveMIMO methodology. The volume of fluid (VOF) multiphase model was applied to treat the water–air interaction. The results for the water amount accumulated in the device reservoir showed that the fluid-dynamic behavior of the overtopping converter has significant differences when comparing the two proposed approaches. Differences up to 240% were found for the water mass accumulated in the overtopping device reservoir, showing evidence that the results can be overestimated when the overtopping device is analyzed under the incidence of the representative regular waves. Furthermore, for all studied cases, it was possible to approximate the water volume accumulated over time in the overtopping reservoir through a first-degree polynomial function.
H2O2 Induces Calcium and ERMES Complex-Dependent Mitochondrial Constriction and Division as Well as Mitochondrial Outer Membrane Remodeling in Aspergillus nidulans
Authors: Garrido-Bazán Verónica , Aguirre Jesús
Published: August 2022

The dynamin-like protein DnmA and its receptor FisA are essential for H2O2-induced mitochondrial division in Aspergillus nidulans. Here, we show that in the absence of DnmA or FisA, mitochondria show few spontaneous transient constrictions, the frequency of which is extensively increased by H2O2 or the carbonyl cyanide m-chlorophenyl hydrazone (CCCP). While H2O2-induced constrictions are transient, CCCP induces a drastic and irreversible alteration of mitochondrial filaments. H2O2 induces a gradual mitochondrial depolarization, while CCCP-induced depolarization is abrupt. The calcium chelator BAPTA-AM prevents the formation of mitochondrial constrictions induced by either H2O2 or CCCP. H2O2 also induces major rearrangements of the mitochondrial outer membrane, which remain after constrictions dissipate, as well as changes in endoplasmic reticulum (ER) and nuclear morphology. Similar mitochondrial constriction, ER and nuclear morphology changes are detected during the early stages of asexual development. ER and ER-Mitochondria encounter structure (ERMES) complex—composed of proteins Mdm10, Mmm1, Mdm43 and Mdm12—are important for mitochondrial division in Saccharomyces cerevisiae. As the Mdm10 ortholog MdmB was found to be essential in A. nidulans, we evaluated its functions in ΔmdmB terminal mutants and ΔmdmB heterokaryons. ΔmdmB conidia produce a short germ tube that fails to grow further, in which inherited mitochondria become gigantic and round shaped, lacking clear contacts with the ER. In slow-growing ΔmdmB heterokaryotic mycelia, multiple hyphae contain very long mitochondria with high ROS levels, as occur in ΔdnmA and ΔfisA mutants. In this hyphae, H2O2 fails to induce mitochondrial constrictions but not outer mitochondrial membrane reshaping, indicating that these are two separate effects of H2O2. Our results indicate that H2O2 induces a generalized mitochondrial constriction response, prior to actual division, involving gradual depolarization; they also indicate that Ca2+ and the ERMES complex are critical for both mitochondrial constriction and division. This supports a view of mitochondrial dynamics as the result of a cascade of signaling events that can be initiated in vivo by H2O2.
Standardized Rehabilitation or Individual Approach?—A Retrospective Analysis of Early Rehabilitation Protocols after Isolated Posterior Cruciate Ligament Reconstruction
Authors: Memmel Clemens , Koch Matthias , Szymski Dominik , Huber Lorenz , Pfeifer Christian , Knorr Christian , Alt Volker , Krutsch Werner
Published: August 2022

(1) Background: Isolated posterior cruciate ligament (PCL) tears represent a severe type of injury. In hospitals, PCL reconstruction (PCL-R) is less frequently performed than other types of knee surgery. It is unclear whether there is consensus among surgeons on how to perform rehabilitation after PCL-R or if there are different, more individual approaches in daily routines. (2) Methods: Rehabilitation protocols and their main criteria (the progression of weight bearing and range of motion, the use of knee braces, rehabilitation training, and sports-specific training) were retrospectively analyzed after PCL-R. (3) Results: Only 33 of 120 (27.5%) analyzed institutes use rehabilitation protocols after PCL-R. The applied protocols showed vast differences between the individual rehabilitation criteria, especially with regard to the progression of weight bearing and the range of motion. The only standardized recommendations were the obligatory use of knee braces and the general restriction of weight bearing and range of motion immediately post-surgery. Therefore, because of the lack of a consensus about a standardized rehabilitation protocol after PCL-R, no recommendation can be made on one particular protocol. (4) Conclusion: There is no acknowledged standardized rehabilitation protocol after PCL-R. In clinical practice, recommendations are influenced by, i.a., surgeons’ opinions and experience. The lack of scientific evidence on a particular standardized rehabilitation protocol after PCL-R suggests that rehabilitation protocols need to be tailored to the individual patient.
Secure and Efficient WBAN Authentication Protocols for Intra-BAN Tier
Authors: Almuhaideb Abdullah M. , Alghamdi Huda A.
Published: August 2022

Telecare medical information system (TMIS) is a technology used in a wireless body area network (WBAN), which has a crucial role in healthcare services. TMIS uses wearable devices with sensors to collect patients’ data and transmit the data to the controller node via a public channel. Then, the medical server obtains the data from the controller node and stores it in the database to be analyzed. Unfortunately, an attacker can try to perform attacks via a public channel. Thus, establishing a secure mutual authentication protocol is essential for secure data transfer. Several authentication schemes have been presented to achieve mutual authentication, but there are performance limitations and security problems. Therefore, this study aimed to propose two secure and efficient WBAN authentication protocols between sensors and a mobile device/controller: authentication protocol-I for emergency medical reports and authentication protocol-II for periodic medical reports. To analyze the proposed authentication protocols, we conducted an informal security analysis, implemented BAN logic analysis, validated our proposed authentication protocol using the AVISPA simulation tool, and conducted a performance analysis. Consequently, we showed that our proposed protocols satisfy all security requirements in this study, attain mutual authentication, resist active and passive attacks, and have suitable computation and communication costs for a WBAN.