bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Characterization of Magnetic Nanoemulsion-Based Senolytic Peptides for Osteoarthritis Treatment
Authors: Zara-Danceanu Camelia-Mihaela , García-Fernández Jenifer , Herea Dumitru-Daniel , Gherca Daniel , de Francisco Carrera Irene , Labusca Luminita , de la Fuente Maria
Published: February 2025

The formulation and characterization of a novel nanoemulsion (NE) delivery system for senomodulator peptides aimed at enhancing the treatment of osteoarthritis (OA) are reported, in combination with magnetic nanoparticles (MNPs), for improving targeted delivery and traceability. Osteoarthritis, a prevalent degenerative joint disease associated with aging, is currently not effectively treated by disease-modifying therapies, posing a consistent health burden on individuals and healthcare systems worldwide. Existing treatments, such as nonsteroidal anti-inflammatory drugs and intra-articular injections, are limited by inadequate local drug concentrations and rapid clearance, often necessitating costly joint replacement. Lipid-based NE composed of biocompatible and biodegradable vitamin E and sphingomyelin, associated with the senolytic peptide NE:TUB1, is able to target senescent cells implicated in OA progression. Improved cellular retention and therapeutic effects of the associated TUB1 peptide, compared to its free form, have been demonstrated, suggesting a significant enhancement in therapeutic potential. The incorporation of MNPs to obtain NE:TUB1-MNP formulations offers the advantage of being traceable in vivo through clinically available imaging technologies, with the potential to enhance targeting capabilities through magnetic guidance. The characterization of NE:TUB1-MNPs involved the assessment of their physical and chemical properties, interaction with cells, cytotoxicity profile, and nanoparticle uptake in vitro using human primary adipose-derived stem cells. NE and NE:TUB1-MNP are shown to be stable, non-toxic, and capable of efficient intracellular uptake. The inclusion of MNPs not only supports cell viability and proliferation but also facilitates medium and long-term product traceability within joints, offering a promising approach for localized treatment. The enhanced anti-senescent role of NE:TUB1-MNP formulations are highlighted, suggesting their potential utility in mitigating OA progression and possibly other degenerative diseases. In conclusion, the study presents a novel therapeutic approach for OA, NE:TUB1-MNPs, leveraging the synergistic effects of peptide-functionalized nanoemulsions and magnetic nanoparticles to improve targeted delivery and therapeutic outcomes. This innovative formulation could pave the way for new treatments for OA and other joint-related conditions, offering significant advancements in regenerative medicine.
Recombinant Humanized Collagen Enhances Secreted Protein Levels of Fibroblasts and Facilitates Rats’ Skin Basement Membrane Reinforcement
Authors: Ye Shijia , Chen Boyu , Jeevithan Lakshmi , Yang Haoze , Kong Yaqi , Diao Xiaozhen , Wu Wenhui
Published: February 2025

Collagen and its peptides exhibit remarkable antioxidant activity, superior biocompatibility, and water solubility, making them a significant research focus in skin care. Hence, the recombinant humanized collagen types I, III, and XVII complexed with niacinamide were developed to address damage in human foreskin fibroblasts (HFF-1) caused by ultraviolet radiation and to evaluate basement membrane proteins in a rat skin model. The Cell Counting Kit-8 (CCK-8) assay showed that higher concentrations of the complex increased the survival of damaged cells by approximately 10% and 22%, respectively, compared to the normal group after 16 and 48 h of treatment. Further biochemical analyses using ELISA and immunofluorescence (IF) confirmed that the complex enhanced the expression of collagen type IV, laminin, P63, and transforming growth factor-β (TGF-β) in the damaged cells. Additionally, the complex boosted the activity of the basement membrane in rat skin and stimulated the secretion of integrin, laminin, and perlecan. Overall, the recombinant humanized collagen complex effectively reinforced the skin’s basement membrane.
Innovations in Wave Energy: A Case Study of TALOS-WEC’s Multi-Axis Technology
Authors: Nasr Esfahani Fatemeh , Sheng Wanan , Ma Xiandong , Hall Carrie M. , Aggidis George
Published: February 2025

The technologically advanced learning ocean system—wave energy converter (TALOS-WEC) project addresses the urgent need for sustainable and efficient energy solutions by leveraging the vast potential of wave energy. This project presents a pioneering approach to wave energy capture through its unique multi-axis and omnidirectional point absorber design. Featuring a fully enclosed power take-off (PTO) system, the TALOS-WEC harnesses energy across six degrees of freedom (DoFs) using an innovative internal reaction mass (IRM) mechanism. This configuration enables efficient energy extraction from the relative motion between the IRM and the hull, aiming for energy conversion efficiencies ranging between 75–80% under optimal conditions, while ensuring enhanced durability in harsh marine environments. The system’s adaptability is reflected in its versatile geometric configurations, including triangular, octagonal, and circular designs, customised for diverse marine conditions. Developed at Lancaster University, UK, and supported by international collaborations, the TALOS-WEC project emphasises cutting-edge advancements in hydrodynamic modelling, geometric optimisation, and control systems. Computational methodologies leverage hybrid frequency-time domain models and advanced panel codes (WAMIT, HAMS, and NEMOH) to address non-linearities in the PTO system, ensuring precise simulations and optimal performance. Structured work packages (WPs) guide the project, addressing critical aspects such as energy capture optimisation, reliability enhancement, and cost-effectiveness through innovative monitoring and control strategies. This paper provides a comprehensive overview of the TALOS-WEC, detailing its conceptual design, development, and validation. Findings demonstrate TALOS’s potential to achieve scalable, efficient, and robust wave energy conversion, contributing to the broader advancement of renewable energy technologies. The results underscore the TALOS-WEC’s role as a cutting-edge solution for harnessing oceanic energy resources, offering perspectives into its commercial viability and future scalability.
Development Strategies for the Mediterranean Coastal Landscape: Adaptive Decision-Making Processes for Implementing the Circular Economy in the Redevelopment of the Reggio Calabria Waterfront
Authors: Della Spina Lucia , Assumma Vanessa
Published: February 2025

This document explores a circular approach to the redevelopment of the city–port system of Reggio Calabria, an area characterized by complex challenges involving economic, social, and environmental needs. By developing a multidimensional decision-making process, three development scenarios were identified to support a sustainable transition. The methodology integrates both quantitative and qualitative assessments, actively involving the local community and stakeholders. The proposed methodology operationalizes the principles of the circular economy by aligning sustainable regeneration scenarios with local needs and environmental constraints. The integrated assessment ensures the applicability of circular models for the resilient redevelopment of the waterfront. The results demonstrate how this model can be applied to other Mediterranean port cities to promote sustainable and resilient regeneration.
Localization of Lesions in Autoimmune Blistering Diseases Is Independent of Site-Specific Target Antigen Expression
Authors: Rastegar Lari Tina , Macias Louis , Robrahn Lara , Dikmen Hasan Onur , Prüßmann Jasper , Kiehne Charlotte , Engster Simon , Weyers Imke , Szymczak Silke , van Beek Nina , Hoffmann Markus H. , Schmidt Enno , Emtenani Shirin
Published: February 2025

Autoimmune blistering diseases (AIBDs) involve autoantibodies targeting proteins in the epidermal/epithelial desmosome (pemphigus) or basement membrane zone (pemphigoid). Despite widespread antigen distribution, lesions exhibit a scattered involvement pattern. This study maps the frequency/severity of AIBD lesions on various body parts and investigates whether differential antigen expression contributes to specific predilection sites. We analyzed affected sites presenting blisters/erosions, erythematous/urticarial lesions, and mucosal lesions in bullous pemphigoid (BP-cohort 1, n = 65; BP-cohort 2, n = 119), pemphigus vulgaris (PV, n = 67), and pemphigus foliaceus (PF, n = 20) patients. To assess antigen expression, we conducted indirect immunofluorescence (IF) staining of 11 AIBD antigens from 13 anatomical sites of 10 body donors without AIBD. In BP, blisters/erosions and erythematous/urticarial lesions predominantly affected arms and legs, while PV/PF patients exhibited frequent involvement of buccal mucosa and back, respectively. IF staining identified significant regional differences in BP180, BP230, and integrin β4 expression, although these variations did not correlate with a higher lesion frequency/severity. Other antigens showed consistent expression across all regions. Our findings suggest that predilection sites for BP and PV/PF are largely unaffected by regional variations in antigen expression but may be influenced by factors like microbiota, mechanical stress, sunlight exposure, local immunity, or genetics.
Improved Calculation of Dynamic Load Capacity for Cylindrical Roller Thrust Bearings: Numerical Update of the Lifetime Reduction Factor η for Bearings with Small and Medium Spin-to-Roll Ratios
Authors: Sauvage Paul , Terwey Torben , Lehmann Benjamin , Jacobs Georg
Published: February 2025

The standard procedures for calculating the lifetime of rolling bearings, defined by DIN ISO 281 and ISO/TS 16281, have been revisited in this work with a specific focus on redefining the η factor for cylindrical roller thrust bearings (CRTBs). The new η factor proposed in this study accounts for the additional spinning motion of the rolling elements on the raceway, which affects the lifetime of thrust roller bearings. By considering different spin-to-roll ratios (SRRs), the revised η factor results in a smaller lifetime reduction, improving from a 42% reduction with η = 0.85 to a 27% reduction with η = 0.91. This modification opens industrial opportunities for bearings that can handle higher loads or feature fewer or smaller rolling elements while maintaining the same lifespan target as bearings sized with the original η factor. An analytical and numerical methodology was developed to calculate the η factor for various bearing configurations. Two bearing geometries were selected to assess the influence of the SRR on bearing life. The methodology integrates calculations of the total friction coefficient, 2D and 3D stress distributions, and lifetime predictions based on subsurface-initiated fatigue failure modes. The numerical results demonstrate the impact of contact stresses and bearing kinematics on η. Although this study was based on numerical simulations, it sets the groundwork for experimental validation. Future work includes experimental testing to validate these findings, with a focus on subscale CRTBs subjected to varying γ values. Accelerated testing strategies, including higher rotational speeds and optimized lubrication, are proposed to enhance the accuracy of the results. These experiments would provide further insights into the life expectancy differences between various configurations, contributing to more precise lifetime calculations for CRTBs.
On the Non-Thermal Mechanisms in Microwave Sintering of Materials
Authors: Lin Ming-Syun , Chu Kwo-Ray
Published: February 2025

The microwave sintering of various materials is a promising technology, which has received much attention for its demonstrated potential. Both the conventional (furnace) and microwave sintering rely on thermal activation for particle bonding, for which a high temperature environment is essential. In comparison, microwave treatment achieves the same degree of densification as furnace sintering in a time shorter by a factor of two or higher and at a temperature lower by 5% to 15%. However, this is a phenomenon not yet fully understood and is commonly referred to as a non-thermal effect. Its understanding is a subject of both physics and practical interest. The non-thermal effect has been studied under years of research in order to broaden the applicability of microwave sintering. Here, we first present an overview of experimentally demonstrated advantages of microwave sintering. To facilitate further studies, we then review the literature and put together four commonly recognized interpretations of the non-thermal effects: the ponderomotive force-driven mass transport, magnetism-created cohesive forces, polarization charge-enhanced wave electric field, and polarization charge-induced attractive force among the sintered particles, with an emphasis on recent development.
The Effect of Proprioceptive Training on Technical Soccer Skills in Youth Professional Soccer
Authors: Eraslan Meriç , Gürkan Alper Cenk , Aydın Serhat , Şahin Musa , Çelik Seyfullah , Söyler Mehmet , Altuğ Tolga , Mülhim Mustafa Alper
Published: February 2025

Background and Objectives: This study analyzes the effects of proprioceptive training on the physical fitness and soccer-specific technical skills of young professional soccer players. Materials and Methods: Twenty-eight male professional soccer players from the Türkiye Football Federation’s Third League, aged 20.46 ± 1.60 years (average), participated voluntarily. Body composition was assessed with the Inbody270 bioelectrical impedance analyzer, while agility and maximal VO2 cardiorespiratory fitness were measured using the ChronoJump smartspeed mat. Soccer-specific technical skill assessments were also conducted. Data were analyzed using SPSS 22, with paired sample t-tests for comparisons, Cohen’s d test for effect size, and repeated measures two-way ANOVA for interaction effects (group × time). Eta squared (η2) values were reported for effect size. A 95% confidence level and p = 0.05 were used. Results: Proprioceptive training significantly improved body fat percentage (p < 0.05; η2 = 0.006) and soccer-specific technical skills, including free juggling (p < 0.05; η2 = 0.302), alternating foot juggling (p < 0.05; η2 = 0.271), right foot juggling (p < 0.05; η2 = 0.250), and shooting performance (p < 0.05; η2 = 0.513). Conclusions: A 12-week proprioceptive training program, when incorporated into soccer practice, significantly enhances soccer-specific technical skills and reduces body fat percentage.
Depression and Its Effect on Geriatric Rehabilitation Outcomes in Switzerland’s Aging Population
Authors: Miletic Bojan , Plisic Antonia , Jelovica Lejla , Saner Jan , Hesse Marcus , Segulja Silvije , Courteney Udo , Starcevic-Klasan Gordana
Published: February 2025

Background and Objectives: Depression is a common mental problem in the older population and has a significant impact on recovery and general well-being. A comprehensive understanding of the prevalence of depressive symptoms and their effects on functional outcomes is essential for improving care strategies. The primary aim of this study was to determine the prevalence of depressive symptoms in older patients undergoing geriatric rehabilitation and to assess their specific impact on their functional abilities. Materials and Methods: A retrospective study was conducted at the Lucerne Cantonal Hospital in Wolhusen, Switzerland, spanning from 2015 to 2020 and including 1159 individuals aged 65 years and older. The presence of depressive symptoms was assessed using the Geriatric Depression Scale (GDS) Short Form, while functional abilities were evaluated using the Functional Independence Measure (FIM) and the Tinetti test. Data analysis was performed using TIBCO Statistica 13.3, with statistical significance set at p < 0.05. Results: Of the participants, 22.9% (N = 266) exhibited depressive symptoms, with no notable differences between genders. Although all patients showed functional improvements, the duration of rehabilitation was prolonged by two days (p = 0.012, d = 0.34) in those with depressive symptoms. Alarmingly, 76% of participants were classified as at risk of falling based on the Tinetti score. However, no significant correlation was found between the GDS and Tinetti scores at admission (p = 0.835, r = 0.211) or discharge (p = 0.336, r = 0.184). The results from the non-parametric Wilcoxon matched-pairs test provide compelling evidence of significant changes in FIM scores when comparing admission scores to those at discharge across all FIM categories. Conclusions: Depressive symptoms are particularly common in geriatric rehabilitation patients, leading to prolonged recovery time and increased healthcare costs. While depressive symptoms showed no correlation with mobility impairments, improvements in functional status were directly associated with reduced GDS scores. Considering mental health during admission and planning is critical in optimizing rehabilitation outcomes.
Optimizing the Dealkalization Process of Red Mud: Controlling Calcium Compounds to Improve Solid–Liquid Separation Performance
Authors: Zhou Jianfei , Dai Mengmeng , Guan Qingjun , Zeng Hua , Sun Wei , Wang Li
Published: February 2025

The acid neutralization process is widely recognized for its effectiveness in the dealkalization of red mud, and it faces challenges in solid–liquid separation due to the formation of numerous colloidal components. This study investigated the impact of calcium-containing compounds (CaO, CaCl2, CaCO3, and CaSO4) on the solid–liquid separation and the dealkalization efficiency of red mud during the dealkalization process. The sodium leaching efficiency of the red mud reached 95.6% when the red mud was reacted with 8% of sulfuric acid for 10 min with a stirring speed and liquid to solid ratio of 700 r/min and 5:1, respectively. The replacement of sulfuric acid using simulated waste acid reached similar sodium leaching efficiency. However, the filtration rate of red mud becomes exceedingly sluggish using sulfuric acid or simulated waste acid. Adding calcium-containing compounds significantly augments the efficacy of solid–liquid separation in red mud. With a mass content of 2% for CaO or 8% for CaCl2, the filtration speed experienced a remarkable fivefold and ninefold increase, respectively. Furthermore, a simplification in the composition was observed within the leaching solution derived from red mud, thereby creating favorable conditions for the extraction of sodium. The influence mechanism was investigated with X-ray diffraction, inductively coupled plasma analysis, and scanning electron microscopy. The addition of calcium compounds led to the formation of calcium silicate and iron silicate in the leaching residue, inhibiting the generation of colloidal substances, such as silica gel. Additionally, these compounds increased the size of red mud particles, facilitating the solid–liquid separation process. This study provides valuable technical insights for the dealkalization of red mud.
Catalase-Knockout Complements the Radio-Sensitization Effect of Titanium Peroxide Nanoparticles on Pancreatic Cancer Cells
Authors: Tasia Winda , Washio Amane , Yamate Koki , Morita Kenta , Mori Yutaro , Kahar Prihardi , Sasaki Ryohei , Ogino Chiaki
Published: February 2025

In previous studies, titanium peroxide nanoparticles (PAA-TiOx NPs) with surfaces functionalized using polyacrylic acid (PAA) and hydrogen peroxide (H2O2) demonstrated a synergistic effect when combined with X-ray irradiation. The combination generated H2O2 and reactive oxygen species (ROS) that enhanced the irradiation efficacy. In the present study, we examined the relationship between catalase and PAA-TiOx NPs sensitization to X-ray radiation because catalase is the primary antioxidant enzyme that converts H2O2 to water and oxygen. Catalase-knockout PANC-1 (dCAT) cells were generated using the CRISPR/Cas9 system, which was confirmed by the suppression of catalase expression in mRNA and protein levels that resulted in an 81.7% decrease in catalase activity compared with levels in wild-type cells. Catalase deficiency was found to increase the production of ROS, particularly in hypoxia. Also, the combination of PAA-TiOx NPs and X-ray 5 Gy resulted in a 7-fold decrease in the survival fraction (SF; p < 0.01) of dCAT cells compared with rates documented in wild-type cells. Interestingly, the combination treatment with X-ray 3 Gy in dCAT cells resulted in an SF similar to that observed in wild-type cells treated with the same combination but at a higher radiation dose (5 Gy). These results suggest that a strategy of catalase inhibition could be used to establish an advanced combination treatment of PAA-TiOx NPs and X-ray irradiation for pancreatic cancer cells.
Machine Learning Descriptors for CO2 Capture Materials
Authors: Orhan Ibrahim B. , Zhao Yuankai , Babarao Ravichandar , Thornton Aaron W. , Le Tu C.
Published: February 2025

The influence of machine learning (ML) on scientific domains continues to grow, and the number of publications at the intersection of ML, CO2 capture, and material science is growing rapidly. Approaches for building ML models vary in both objectives and the methods through which materials are represented (i.e., featurised). Featurisation based on descriptors, being a crucial step in building ML models, is the focus of this review. Metal organic frameworks, ionic liquids, and other materials are discussed in this paper with a focus on the descriptors used in the representation of CO2-capturing materials. It is shown that operating conditions must be included in ML models in which multiple temperatures and/or pressures are used. Material descriptors can be used to differentiate the CO2 capture candidates through descriptors falling under the broad categories of charge and orbital, thermodynamic, structural, and chemical composition-based descriptors. Depending on the application, dataset, and ML model used, these descriptors carry varying degrees of importance in the predictions made. Design strategies can then be derived based on a selection of important features. Overall, this review predicts that ML will play an even greater role in future innovations in CO2 capture.
Carboxyethylsilanetriol-Functionalized Al-MIL-53-Supported Palladium Catalyst for Enhancing Suzuki–Miyaura Cross-Coupling Reaction
Authors: Liang Yucang , Ning Xin , Zhen Yanzhong
Published: February 2025

The application of metal–organic frameworks (MOFs) has attracted increasing attention in organic synthesis. The modification of MOFs can efficiently tailor the structure and improve the property for meeting ongoing demand in various applications, such as the alteration of gas adsorption and separation, catalytic activity, stability, and sustainability or reusability. In this study, carboxyethylsilanetriol (CEST) disodium salt was used as a dual-functional ligand for modified Al-MIL-53 to fabricate CEST-functionalized Al-MIL-53 samples through a hydrothermal reaction of aluminum nitrate, terephthalic acid, and CEST disodium salt by varying the molar ratio of CEST to terephthalic acid and keeping a constant molar ratio of Al3+/-COOH of 1:1. The structure, composition, morphology, pore feature, and stability were characterized by XRD, different spectroscopies, electron microscopy, N2 physisorption, and thermogravimetric analysis. With increasing CEST content, CEST-Al-MIL-53 still preserves an Al-MIL-53-like structure, but the microstructure changed compared with pure Al-MIL-53 due to the integration of CEST. Such a CEST-Al-MIL-53 was used as the support to load Pd particles and afford a catalyst Pd/CEST-Al-MIL-53 for Suzuki–Miyaura C-C cross-coupling reaction of aryl halides and phenylboronic acid under basic conditions. The resulting Pd/CEST-Al-MIL-53 showed a high catalytic activity compared with Pd/Al-MIL-53, due to the nanofibrous structure of silicon species-integrated CEST-Al-MIL-53. The nanofiber microstructure undergoes a remarkable transformation into intricate 3D cross-networks during catalytic reaction, which enables the leachable Pd particles to orientally redeposit and inlay into these networks as the monodisperse spheres and thereby effectively preventing Pd particles from aggregation and leaching, therefore demonstrating a high catalytic performance, long-term stability, and enhanced reusability. Obviously, the integration of CEST into MOFs can effectively prevent the leaching of active Pd species and ensure the re-deposition during catalysis. Moreover, catalytic performance strongly depended on catalyst dosage, temperature, time, solvent, and the type of the substituted group on benzene ring. This work further extends the catalytic application of hybrid metal–organic frameworks.
A Reporter Gene Assay for Measuring the Biological Activity of PEGylated Recombinant Human Growth Hormone
Authors: Hu Shaowang , Zhang Xiaoming , Li Yi , Li Jing , Wang Yingwu , Liang Chenggang
Published: February 2025

PEGylated recombinant human growth hormone (PEG-rhGH) has garnered significant interest in growth hormone research due to its prolonged half-life and improved patient compliance. An accurate evaluation of its biological activity is critical for ensuring the quality of PEG-rhGH-based therapeutics. In this study, we established an in vitro bioactivity assay using a reporter gene method based on the HepG2/IGF-1 cell line. Key assay parameters, including the initial concentration of PEG-rhGH, serial dilution ratios, cell density, and incubation time, were systematically optimized to generate robust dose–response curves. The assay demonstrated high sensitivity, precision, and reproducibility across multiple batches of PEG-rhGH. The validation results showed an excellent correlation with traditional in vivo animal studies and the Nb2-11 cell proliferation assay, highlighting its suitability for quality control. Furthermore, we developed an ion exchange chromatography (IEC) method to separate five positional isomers of PEG-rhGH, revealing significant differences in bioactivity depending on the PEG modification site. This study demonstrates that the optimized reporter gene assay is not only effective for quality control of PEG-rhGH but also serves as a valuable tool for evaluating and optimizing PEGylated long-acting growth hormone therapeutics.
Effect of Different Dietary Patterns on Patients with Depressive Disorders: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Tavakoly Rahele , Moosburner Alina , Anheyer Dennis , Cramer Holger
Published: February 2025

Objectives: This study aimed to investigate the effects of different dietary patterns on depressive disorders. Methods: PubMed/MEDLINE, Cochrane CENTRAL, Embase, PsycINFO, Scopus, and ProQuest databases were systematically searched until 30 April 2024 for randomized controlled trials (RCTs) assessing the effects of different dietary patterns on depressive symptoms in adults with depressive disorders. Secondary outcomes included remission rates, quality of life, and safety. Standardized mean difference (SMD) and 95% confidence intervals (CIs) were aggregated using a random-effects model. Study quality was assessed with the Cochrane Risk of Bias (RoB)-2 tool, and certainty of evidence was determined using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach. Results: Five RCTs (n = 952) were included, all investigating the Mediterranean diet (MD) in individuals with major depressive disorder or elevated depression levels. The analysis found no significant effect of short-term MD intervention on depression severity compared to active (SMD = −1.25 [95% CI: −5.11 to 2.61]) or passive (SMD = −0.22 [95% CI: −0.74 to 0.29]) controls. There was no effect on quality of life compared to active controls (SMD = 0.71 [95% CI = −3.38 to 4.79]). Intermediate and long-term results were similar. The overall risk of bias was “some concerns”, and the certainty of evidence was “very low” for most of the results. Conclusions: The MD appears to have no potential influence on major depressive disorder. However, this finding should be interpreted cautiously due to the limited number of RCTs. Further studies on dietary patterns and depressive disorders are needed for more robust conclusions. Systematic Review Registration: PROSPERO registration no. CRD42024541885.
A Comparative Effect of 12-Week Dietary Intervention of Policosanol (Raydel®) and Red Yeast Rice (RYR, Kobayashi) in Managing Dyslipidemia and Organ Damage in Hyperlipidemic Zebrafish
Authors: Cho Kyung-Hyun , Bahuguna Ashutosh , Kim Ji-Eun , Lee Sang Hyuk , Lee Yunki , Jeon Cheolmin
Published: February 2025

Background: A comparative 12-week dietary intervention of red yeast rice (RYR, Beni-koji, Kobayashi, Japan) and Cuban policosanol (PCO, Raydel®, Thornleigh, Australia) was assessed for dyslipidemia, antioxidant status, and vital organ functionality in hyperlipidemic zebrafish. Methods: Hyperlipidemic zebrafish were supplemented with a high-cholesterol diet (HC, final 4%, w/w) infused with either a powdered RYR tablet (final 1.0%, w/w), a PCO tablet (final 1.0%, w/w), or a combination of 0.5% (w/w) each of RYR and PCO powder for 12 weeks. Subsequently, blood and organs were collected and processed for biochemical and histological examination. Results: RYR and PCO consumption showed a substantial effect against HC-induced hyperlipidemia by reducing the total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C). Exclusively, PCO supplementation revealed a significant elevation in the HC-diminished high-density lipoprotein cholesterol (HDL-C). In addition, PCO supplementation showed a significant elevation in plasma ferric ion-reducing ability (FRA) and sulfhydryl content, as well as alleviating the blood glucose level of hyperlipidemic zebrafish. The most noteworthy impact, with a significant two-fold (p < 0.001) augmentation of HC-diminished plasma paraoxonase (PON) activity, was observed in response to PCO. In contrast, the RYR supplementation failed to establish curative effects against HC-disturbed plasma antioxidant variables and blood glucose levels. The histological outcome revealed a severe toxicological impact of the RYR on the liver, reflected by fatty liver changes and three-fold heightened IL-6 production compared to HC control. Contrastingly, PCO exhibited significant hepatoprotection and effectively neutralized the hepatic toxicity triggered by HC and RYR. Also, RYR showed kidney atrophy, intense ROS generation, apoptosis, and senescence. Conversely, the PCO supplementation protected the kidney from HC- and RYR-induced toxicity. Likewise, PCO supplementation notably alleviated histological alterations and oxidative stress in the brain, ovary, and testis of hyperlipidemic zebrafish. Conclusions: This comparative study establishes PCO’s therapeutic effect against the challenges posed by HC, while RYR emerged with serious toxicological concerns towards the liver, kidney, and other organs of hyperlipidemic zebrafish.
Cellular Epigenetic Targets and Epidrugs in Breast Cancer Therapy: Mechanisms, Challenges, and Future Perspectives
Authors: Alalhareth Ibrahim S. , Alyami Saleh M. , Alshareef Ali H. , Ajeibi Ahmed O. , Al Munjem Manea F. , Elfifi Ahmad A. , Alsharif Meshal M. , Alzahrani Seham A. , Alqaad Mohammed A. , Bakir Marwa B. , Abdel-Wahab Basel A.
Published: February 2025

Breast cancer is the most common malignancy affecting women, manifesting as a heterogeneous disease with diverse molecular characteristics and clinical presentations. Recent studies have elucidated the role of epigenetic modifications in the pathogenesis of breast cancer, including drug resistance and efflux characteristics, offering potential new diagnostic and prognostic markers, treatment efficacy predictors, and therapeutic agents. Key modifications include DNA cytosine methylation and the covalent modification of histone proteins. Unlike genetic mutations, reprogramming the epigenetic landscape of the cancer epigenome is a promising targeted therapy for the treatment and reversal of drug resistance. Epidrugs, which target DNA methylation and histone modifications, can provide novel options for the treatment of breast cancer by reversing the acquired resistance to treatment. Currently, the most promising approach involves combination therapies consisting of epidrugs with immune checkpoint inhibitors. This review examines the aberrant epigenetic regulation of breast cancer initiation and progression, focusing on modifications related to estrogen signaling, drug resistance, cancer progression, and the epithelial–mesenchymal transition (EMT). It examines existing epigenetic drugs for treating breast cancer, including agents that modify DNA, inhibitors of histone acetyltransferases, histone deacetylases, histone methyltransferases, and histone demethyltransferases. It also delves into ongoing studies on combining epidrugs with other therapies and addresses the upcoming obstacles in this field.
Testing Protocols and Procedures for Undertaking Fire Resistance Tests on Concrete Structures Incorporating Fiber-Reinforced Polymers
Authors: Kodur Venkatesh , Naser M. Z. , Kim Hee Sun
Published: February 2025

Fiber-reinforced polymers (FRPs) are often incorporated as internal (primary) reinforcement in new concrete constructions or as external (secondary) reinforcement in retrofitting and strengthening of existing concrete structures. Under fire conditions, the response of FRP-incorporated concrete structures are altered due to the presence of FRPs; thus, their fire performance is different from that of concrete structures with conventional metallic reinforcement. However, the fire resistance of these FRP-incorporated structural members continues to be evaluated through standard fire resistance tests, which are similar to conventional steel and concrete structural members. Despite the complexity of this testing approach and its drawbacks, standard fire testing remains a cornerstone in evaluating FRP-incorporated concrete structural members. Thus, this paper sheds more light on the fire testing procedure and discusses the distinctive factors that differentiate the fire performance of FRP-incorporated concrete structures from that of conventional concrete structures and the need for additional provisions to test such structures. To address the current shortcomings, a set of additional testing protocols and procedures for undertaking fire resistance tests on FRP-incorporated concrete structural members are presented. The performance criteria to be applied to evaluate the failure of FRP–RC structural members under fire conditions are discussed.
Influence of Foaming Agents and Stabilizers on Porosity in 3D Printed Foamed Concrete
Authors: Rudziewicz Magdalena , Maroszek Marcin , Hutyra Adam , Góra Michał , Rusin-Żurek Karina , Hebda Marek
Published: February 2025

This study examines the pore structure and distribution in 3D printed and cast foamed concrete using protein-based and synthetic foaming agents alongside various stabilizing additives. In 3D printed samples, pores are irregular and flattened due to mechanical forces during printing, whereas cast samples display uniform, spherical pores from homogeneous foam distribution. Samples containing the CA stabilizer show higher apparent densities (up to 2.05 g/cm3 for printed samples), correlating with lower water absorption. Protein-based foaming agents (PS) produce smaller, more evenly distributed pores, while synthetic agents (AS) result in larger, less uniform pores. Stabilizers significantly influence pore characteristics: commercial stabilizers yield smaller, more uniform pores, while recycled industrial oil (UO) leads to larger, more variable pores. Protein-based agents improve structural stability and reduce water absorption through uniform pore distributions, while synthetic agents lower density and increase water absorption. The highest sorption values were observed in samples with AS without stabilizer (1.7 kg/m2h1/2) and AS and UO (1.6 kg/m2h1/2) in a vertical orientation, with the horizontal orientation of sample AS and UO achieving a peak value of 2.0 kg/m2h1/2. Moreover, stabilization using UO resulted in higher sorption coefficients than stabilization with CA. High porosity in M1 resulted in low strength (0.2 MPa bending, 0.1 MPa perpendicular compression), while M5 showed superior performance (11.5 MPa perpendicular compression). PS-foamed samples (M4, M6) with uniform pores had the highest strengths, with M6 achieving 3.8 MPa bending and 10.3 MPa perpendicular compression. Perpendicular compression (M5: 11.5 MPa) was up to three times stronger than parallel compression due to weak interlayer bonds in 3D printing.
Assessment of the Applicability of Waste Concrete Fine Powder as a Raw Material for Cement Clinker
Authors: Shin Sangchul , Kim Geonwoo , Kim Jinman , Kim Haseog , Park Sungyu
Published: February 2025

The cement industry is responsible for a significant portion of global CO2 emissions, primarily due to the decarbonatization of limestone during clinker production. To mitigate this environmental impact, this study investigated the feasibility of using waste concrete fine powder, produced during the recycling of waste concrete, as a decarbonized raw material in cement clinker production. As a decarbonized material, waste concrete fine powder presents a valuable opportunity to reduce CO2 emissions typically produced during the decarbonatization of limestone in clinker production. In addition, its use supports the recycling of construction waste, contributing to both emissions reduction and resource sustainability. In this study, samples were collected from 20 intermediate treatment plants in South Korea, where the chemical composition, particle size distribution, and carbonation rate of the fine powders were analyzed. The experimental results show that the properties of waste concrete fine powder vary significantly depending on the recycling process. Road construction aggregate production plants, which typically involve two to three crushing stages, produce fine powders with higher CaO content (28–31%) and consistent particle size distributions. In contrast, plants producing aggregates for concrete, which involve four to six crushing stages, produce powders with lower CaO content (around 20%) and greater variability in particle size. The average carbonation rate of 7.44% suggests that these fine powders can replace limestone in clinker production. It is estimated that substituting 5% of limestone with waste concrete fine powder could reduce CO2 emissions from limestone decarbonatization by approximately 952,560 tons in 2023, representing a 3.34% decrease in total emissions from clinker production. However, it is important to note that the CO2 emissions reduction calculation is not from a lifecycle perspective, without considering the energy-related emissions from recycling waste concrete fine powder. Nevertheless, this study highlights the potential for waste concrete fine powder to serve as a sustainable raw material for the cement industry, contributing to both CO2 reduction and efficient recycling of construction waste.
Evaluating Generalization of Methods for Artificially Generating NDVI from UAV RGB Imagery in Vineyards
Authors: Doornbos Jurrian , Babur Önder , Valente João
Published: February 2025

High-resolution NDVI maps derived from UAV imagery are valuable in precision agriculture, supporting vineyard management decisions such as disease risk and vigor assessments. However, the expense and complexity of multispectral sensors limit their widespread use. In this study, we evaluate Generative Adversarial Network (GAN) approaches—trained on either multispectral-derived or true RGB data—to convert low-cost RGB imagery into NDVI maps. We benchmark these models against simpler, explainable RGB-based indices (RGBVI, vNDVI) using Botrytis bunch rot (BBR) risk and vigor mapping as application-centric tests. Our findings reveal that both multispectral- and RGB-trained GANs can generate NDVI maps suitable for BBR risk modelling, achieving R-squared values between 0.8 and 0.99 on unseen datasets. However, the RGBVI and vNDVI indices often match or exceed the GAN outputs, for vigor mapping. Moreover, model performance varies with sensor differences, vineyard structures, and environmental conditions, underscoring the importance of training data diversity and domain alignment. In highlighting these sensitivities, this application-centric evaluation demonstrates that while GANs can offer a viable NDVI alternative in some scenarios, their real-world utility is not guaranteed. In many cases, simpler RGB-based indices may provide equal or better results, suggesting that the choice of NDVI conversion method should be guided by both application requirements and the underlying characteristics of the subject matter.
A Three-Dimensional Imaging Method for Unmanned Aerial Vehicle-Borne SAR Based on Nested Difference Co-Arrays and Azimuth Multi-Snapshots
Authors: Shi Ruizhe , Luo Yitong , Zhang Zhe , Qiu Xiaolan , Ding Chibiao
Published: February 2025

Due to its miniature size and single-pass nature, Unmanned Aerial Vehicle (UAV)-borne array synthetic aperture radar (SAR) is capable of obtaining three-dimensional (3D) electromagnetic scattering information with a low cost and high efficiency, making it widely applicable in various fields. However, the limited payload capacity of the UAV platform results in a limited number of array antennas and affects 3D resolution. This paper proposes a 3D imaging method for UAV-borne SAR based on nested difference co-arrays and azimuth multi-snapshots. We first designed an antenna arrangement based on nested arrays, generating a virtual antenna twice as long as the original one. Then, we used a difference co-array method for 3D imaging. The required multi-snapshot data were obtained through azimuth down-sampling, rather than traditional spatial averaging methods. Due to the slow flight of the UAV, this method could generate multiple SAR images without affecting the two-dimensional resolution. Based on simulations and real data verification, the proposed algorithm overcomes the problem of two-dimensional resolution decline caused by traditional spatial averaging methods and improves three-dimensional resolution ability, theoretically achieving half the Rayleigh resolution.
Chromium Remediation from Tannery Wastewater in Arequipa, Peru: Local Experiences and Prospects for Sustainable Solutions
Authors: Morales-Paredes Lino F. , Garcia-Chevesich Pablo A. , Romero-Mariscal Giuliana , Arenazas-Rodriguez Armando , Ticona-Quea Juana , Tejada-Purizaca Teresa R. , Vanzin Gary , Sharp Jonathan O.
Published: February 2025

The release of tannery wastewater contributes to chromium (Cr) pollution globally. Herein, we conduct a novel consolidation of research from the Arequipa region of southern Peru that integrates university theses written in Spanish alongside peer-reviewed journal articles. The objective is to provide a place-based complement to existing research in English scientific journals focused on effective tools for Cr treatment from tannery wastewater. Our consolidation categorized a total of 75 publications (70 theses and five peer-reviewed) into five distinct strategies for Cr treatment: adsorption (twenty-three studies), phytoremediation (eighteen studies), bioremediation (thirteen studies), electrocoagulation (five studies), and other techniques (fifteen studies). This synthesis highlighted potentially promising approaches that could be sustainably tailored to regional resources and waste products. This includes sorptive materials derived from food waste such as native achiote peels (B. orellana) and avocado seeds (P. americana) either used directly or as a feedstock for biochar. Other technologies include phytoremediation using microalgae and resident vascular plants and microbial bioremediation that capitalizes on indigenous bacteria and fungi. Promise was also discerned in studies that incorporated a combination of abiotic and biotic mechanisms tailored toward the region, such as infiltration using selective and bioactive materials, wetlands, solar distillation, iron-based coagulation and flocculation, and bioreactors. These findings provide a sustainable complement to prior global investigations for effective attenuation strategies by adding novel materials and techniques that could be further explored to assess the viability of implementation at pilot and larger scales. These promising technologies and the ability to tailor sustainable treatments toward local resources highlight the opportunity to prioritize the treatment of tannery wastewater to ensure a cleaner environment by informing policy makers, academics, and industry on technologies that could be adopted for implementation in the region.
Creation of Wind Speed Maps and Determination of Wind Energy Potential with Geographic Information Systems: The Case of Kırklareli Province, Türkiye
Authors: Karataş Kamil , Bıçakcı Celal
Published: February 2025

The intensive use of fossil fuels for energy production harms the environment. The adoption of sustainable energy systems can reduce the damage. Wind energy is one of the most widely used renewable sources. The most important problem in establishing new wind power plants (WPPs) is estimating the wind energy potential (WEP) in potential installation locations where there are no measured data. Many geographic information system (GIS)-based studies have been conducted on this subject. In this study, based on the technical specifications of a wind turbine selected for the Kırklareli Province of Türkiye, wind speed maps at 125 m height were created using many station points with known locations and wind speeds and the WEP of Kırklareli was calculated. In addition, the WEP map of Kırklareli was created by first determining the areas where WPPs cannot be installed and creating the wind speed map. After removing exclusion areas where wind turbines cannot be installed, the wind speeds at 125 m ranged between 3.12 m/s and 8.51 m/s. The wind speed was found to be higher in the south of the province, and the total WEP in areas with wind speeds higher than 6 m/sec was 6628.21 MW.
ESG Policy–Practice Decoupling: A Measurement Framework and Empirical Validation
Authors: Loko Atta Guy Sylvestre , Schiehll Eduardo
Published: February 2025

As sustainability becomes more critical to corporate strategy and performance, firms, investors, and researchers must continue to refine methods for measuring and addressing the gap between rhetoric and reality. Closing this gap is crucial to ensuring that externally oriented ESG claims are supported by genuine internal actions that benefit both the firm and society at large. To address this issue, this study introduces a theoretically driven framework to assess the alignment (or lack thereof) between firms’ ESG policies and their actual implementation. By proposing a more granular and objective measure, we address a gap in the existing literature. Additionally, we empirically validate this framework using data from ASSET4, providing insights into the extent and persistence of this phenomenon using a sample of S&P 1500 firms from 2016 to 2022. Our results reveal that misalignment between internal actions and external endorsements in managing environmental and social issues is both significant and persistent across the years analyzed. Over 80% of the sample firms exhibit this misalignment, underscoring its prevalence within the sample. In more recent years, however, firms have shown a clear tendency to prioritize internal actions over initiatives aimed at externally endorsing their efforts. Building on the framework we propose to measure ESG policy–practice decoupling, along with the empirical analysis we conducted, we discuss its broader implications and outline several opportunities for future research.