Evaluation of biotherapies T.cruzi 15x, 16x, 17x and "potency chords" in experimental infection by Trypanosoma cruzi.
Authors: Ferraz Fabiana Nabarro , GonÃƒÆ’Ã‚Â§alves Valdir AntÃƒÆ’Ã‚Â´nio , Aleixo Denise Lessa , Mizutani AngÃƒÆ’Ã‚Â©lica Sayuri , AraÃƒÆ’Ã‚Âºjo Silvana Marques de 
Published: December 2021

Background: The biotherapies are drugs widely utilized against infectious diseases. BiotherapiesÃƒÂ¢Ã¢â€šÂ¬Ã¢â€žÂ¢ profylatic and therapeutic action against Chagas Disease is currently being investigated, but it is needed to develop further controlled experiments ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œin vivoÃƒÂ¢Ã¢â€šÂ¬Ã‚Â, which could define more clearly: dilution, dose, time of use and, if possible, the action mechanisms of these ultradiluted medicaments [1,2].

Aim: Evaluate the effect biotherapies T. cruzi 15x, 16x, 17x and ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œpotency chordsÃƒÂ¢Ã¢â€šÂ¬Ã‚Â, on experimental infection by T. cruzi.

Methodology: A blind, controlled and randomized by drawing test was performed. Animals: 29 male Swiss mice, four weeks old were utilized. The animals were kept at Parasitology Vivarium/State University of Maringá (UEM), in ideal conditions of temperature (22Ãƒâ€šÃ‚Â±2)Ãƒâ€šÃ‚ÂºC and photoperiod (light / dark cycle 12h). Mice have been inoculated intraperitoneally with 1400 blood trypomastigotes Y strain and divided in groups: IC ÃƒÂ¢Ã¢â€šÂ¬Ã¢â‚¬Å“ Infection control (treated with distilled water ÃƒÂ¢Ã¢â€šÂ¬Ã¢â‚¬Å“ 9 animals); TBBA15x3days ÃƒÂ¢Ã¢â€šÂ¬Ã¢â‚¬Å“ Treated with biotherapy 15x 3 days before and 3 days after infection (5 animals); TBBA16x3days ÃƒÂ¢Ã¢â€šÂ¬Ã¢â‚¬Å“ Treated with biotherapy 16x 3 days before and 3 days after infection (5 animals); TBBA17x3days ÃƒÂ¢Ã¢â€šÂ¬Ã¢â‚¬Å“ Treated with biotherapy 17x 3 days before and 3 days after infection (5 animals); TBBAChords3days ÃƒÂ¢Ã¢â€šÂ¬Ã¢â‚¬Å“ Treated with biotherapy 15x, 16x, 17x ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œpotency chordsÃƒÂ¢Ã¢â€šÂ¬Ã‚Â, 3 days before and 3 days after infection (5 animals). Biotherapies: prepared by a homeopathic pharmacist from UEM, according to FarmacopÃƒÆ’Ã‚Â©ia Homeopática Brasileira [3]. Biotherapies treatment schedule: diluted in distilled water (10Ãƒâ€šÃ‚ÂµL/mL in ambar bottles ÃƒÂ¢Ã¢â€šÂ¬Ã¢â‚¬Å“ renewed each two days) offered ad libitum, 3 days before and 3 days after infection in all groups. Parasitological parameters: parasitemia was assessed from infection until death, according to BrenerÃƒÂ¢Ã¢â€šÂ¬Ã¢â€žÂ¢s technique [4] with 5Ãƒâ€šÃ‚ÂµL of blood collected from the tail vein and examined in optical microscope. Pre-patent period, patent period, total parasitemia, survival and morbidity were obtained from the parasitemia curve. Clinical parameters: Visually assessed (presence or absence): body hair aspect (bristling), edema, movement and diarrhea. Measured: body weight, temperature, food and water intake. Ethics: This study has been approved by the UEM Ethics Committee for Experiments in Animals - Registration 030/2008. Statistical analysis: was performed using the tests Kruskal Wallis and Mann-Whitney tests, significance of 5%.

Results: There was not statistical difference between total parasitemia of the groups treated with biotherapies and the IC group (p=0.6819). The parasitemia curve of group TBBAChords3days was greater then the IC (p=0.0418). Despite this increase, patent period and mortality both showed a decreasing tendency, while pre patent period and survival time increased (p=0.373). The same tendency results were observed for TBBA17x3days results (Table 1). Survival of at least one mice in groups TBBA17x3days and TBBAChords3days is worthy of discussion, since Y strain causes 100% mortality in these experimental conditions.

Groups TBBA17x3days and TBBAChords3days showed better evolution than IC group for body weight, temperature, food and water intake (p=0.05), body hair aspect and edema developing. Diarrhea and hind legs paralysis were only observed in mice belonging to groups IC and TBAA16x3days.

Conclusions: Superior effect was obtained with biotherapies 17x and ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œPotency ChordsÃƒÂ¢Ã¢â€šÂ¬Ã‚Â, both for clinical and parasitological parameters. ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œPotency chordsÃƒÂ¢Ã¢â€šÂ¬Ã‚Â has proper effect which distinguishes it from the individual effects of the dilutions that compound it.