bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Characterization, Clinical Significance, Tumor Microenvironment Association and Drug Susceptibility of DNA Methylation-Driven Genes in Renal Cell Carcinoma
Authors: Wang Jinpeng , Wu Pengfei , Zhang Wei , Hou Wenbin , Zhao Enyang , Li Xuedong
Published: June 2021

Background: Accumulating evidence suggests that DNA methylation has essential roles in the development of renal cell carcinoma (RCC). Aberrant DNA methylation acts as a vital role in RCC progression through regulating gene expression, yet little is known about the role of methylation and its association with prognosis in RCC. The purpose of this study is to explore the DNA methylation-driven genes for establishing prognostic-related molecular clusters and providing a basis for survival prediction.Methods: Differentially expressed genes (DEGs) were calculated by the “limma” R package. DNA methylation-driven genes (MDGs) were identified using the “methylMix” R package. The differentially expressed DNA methylation-driven genes (DEMDGs) were obtained by intersecting MDGs and DEGs. RCC data sets were divided into two groups using “ConsensusClusterPlus” R packet. The prognostic model was constructed based on multivariate Cox regression analysis. Based on the optimal cut-off value of the risk score, the patients were divided into high-risk group and low-risk group. The potential molecular mechanisms of prognostic-related DEMDGs were detected by GO, KEGG and GSEA analysis.Results: In this study, 146 DEMDGs were selected and two clusters were distinguished by consensus clustering. We further evaluated the immune status of two clusters and selected 106 DEGs in cluster 1. Functional enrichment analysis of 106 DEGs and cluster-based immune status analysis provided new insights for the development of RCC. The prognostic model based on 17 DEMDGs was constructed to predict prognosis of RCC patients. The predictive nomogram and the web-based survival rate calculator (http://127.0.0.1:7634/) were built to validate predictive accuracy of prognostic model. Furthermore, the risk scores were strongly associated with clinical features, immune status and drug susceptibility.Conclusion: Novel prognosis-related clusters based on the 146 DEMDGs were constructed and might contribute to precision medicine development. The 17 DEMDGs were utilized to construct a prognostic model for RCC prediction, which was significantly correlated with prognosis, immune infiltration, clinical features and drug sensitivity.
The non-brain anterior nerve center and tentacle crown structure of Owenia borealis (Annelida, Oweniidae): the evolution of the nervous system and tentacles in Bilateria
Authors: Rimskaya-Korsakova Nadezhda , Dyachuk Vyacheslav , Temereva Elena
Published: June 2021

The Oweniidae are marine annelids with many unusual features of organ system, development, morphology, and ultrastructure. Together with magelionds, oweniids have been placed within the Palaeoannelida, a sister group to all remaining annelids. The study of this group may increase our understanding of the early evolution of annelids (including their radiation and diversification) and of the morphology of the last common bilaterian ancestor. In the current research, scanning electron microscopy revealed that the tentacle apparatus consists of 10 branched arms. The tentacles are covered by monociliary cells that form a ciliar groove that extends along the oral side of the arm base. Light, confocal, and transmission electron microscopy revealed that head region contains two circular intraepidermal nerves (outer and inner) that give rise to the neurites of each tentacle, i.e., intertentacular neurites are absent. Each tentacle contains a coelomic cavity with a network of blood capillaries. Monociliar myoepithelial cells of the tentacle coelomic cavity form both the longitudinal and the circular muscles. The structure of this myoepithelium is intermediate between simple and pseudo-stratified myepithelium. Overall, tentacles lack prominent zonality, i.e., co-localization of ciliary zones, neurite bundles, and muscles. This organization, which indicates a non-specialized tentacle crown in O. borealis and other oweniids with tentacles, is probably ancestral for annelids and for all Bilateria. The outer circular nerve of O. borealis is a dorsal medullary commissure that apparently functions as an anterior nerve center and is organized at the ultrastructural level as a stratified neuroepithelium. Given the hypothesis that the anterior nerve center of the last bilateral ancestor might be a diffuse neural plexus network, these results suggest that the ultra anatomy of that plexus brain might be a stratified neuroepithelium. Alternatively, the results could reflect the simplification of structure of the anterior nerve center in some bilaterian lineages.
Design and development of nanostructured co delivery of artemisinin and chrysin for targeting hTERT gene expression in breast cancer cell line: possible clinical application in cancer treatment
Authors: Azar Leila Khoshravan , Dadashpour Mehdi , Firouzi-Amandi Akram , Zarghami Nosratollah
Published: June 2021

Background: Breast cancer is one of the most significant causes of female cancer death worldwide. To explore the possibility of a novel chemo-preventive strategy for improving breast cancer treatment, the anticancer effects of a combination two natural compounds, Artemisinin (Art) and Chrysin (Chr), against T47D breast cancer cells were investigated.Methods: For this purpose, Art and Chr were co-encapsulated in PEGylated PLGA nanoparticles (NPs) and evaluated for their therapeutic efficacy. The morphology and dynamic light scattering (DLS) analyses were carried out to optimize the Nano formulations. Drug release study was performed using the dialysis method and then the cytotoxic and inhibitory effect of individual and combined drugs on the expression level of hTERT in the T47D breast cell line was evaluated using MTT assay and qPCR, respectively. Results: The results showed that pure drugs and formulations exhibited dose-dependent cytotoxicity against T47D cells and especially, Art/Chr–PLGA/PEG NPs had a more synergistic anti-proliferative effect and significantly arrested the growth of cancer cells than the other groups. Real-time PCR results revealed that Art, Chr and combination of Art–Chr in pure and encapsulated forms inhibited hTERT gene expression. Conclusions: It was found that Art–Chr–PLGA/PEG NPs relative to pure combination could further decline hTERT expression in all concentrations. Our study demonstrated that Art–Chr–PLGA/PEG NPs based combinational therapy holds promising potential for the treatment of breast cancer.
Segmentation of Pancreatic Ductal Adenocarcinoma (PDAC) and Surrounding Vessels in CT Images Using Deep Convolutional Neural Networks and Texture Descriptors
Authors: Mahmoudi Tahereh , Kouzahkanan Zahra Mousavi , Radmard Amir Reza , Kafieh Raheleh , Salehnia Aneseh , Davarpanah Amir H. , Arabalibeik Hossein , Ahmadian Alireza
Published: June 2021

Fully automated and volumetric segmentation of critical tumors may play a crucial role in diagnosis and surgical planning. One of the most challenging tumor segmentation tasks is localization of Pancreatic Ductal Adenocarcinoma (PDAC). Exclusive application of conventional methods does not appear promising. Deep learning approaches has achieved great success in the computer aided diagnosis, especially in biomedical image segmentation. This paper introduces a framework based on convolutional neural network (CNN) for segmentation of PDAC mass and surrounding vessels in CT images by incorporating powerful classic features, as well. First, a 3D-CNN architecture is used to localize the pancreas region from the whole CT volume using 3D Local Binary Pattern (LBP) map of the original image. Segmentation of PDAC mass is subsequently performed using 2D attention U-Net and Texture Attention U-Net (TAU-Net). TAU-Net is introduced by fusion of dense Scale-Invariant Feature Transform (SIFT) and LBP descriptors into the attention U-Net. An ensemble model is then used to cumulate the advantages of both networks using a 3D-CNN. In addition, to reduce the effects of imbalanced data, a new loss function is proposed as a weighted combination of three classic losses including Generalized Dice Loss (GDL), Weighted Pixel-Wise Cross Entropy loss (WPCE) and boundary loss. Due to insufficient sample size for vessel segmentation, we used the above-mentioned pre-trained networks and fin-tuned them. Experimental results show that the proposed method improves the Dice score for PDAC mass segmentation in portal-venous phase by 7.52% compared to state-of-the-art methods in term of DSC. Besides, three dimensional visualization of the tumor and surrounding vessels can facilitate the evaluation of PDAC treatment response.
Population Distribution of Wild Radish (Raphanus Raphanistrum L.) Weed and Soil Depth of Seed Bank Across Different Land-Use Types in Horro District, Western Oromia, Ethiopia
Authors: Shone Geleta , Wakoya Fikiru , Jaleta Zerihun , Legese Hirpha
Published: June 2021

Background: Wild radish (Raphanus raphanistrum L.) is an invasive weed in Ethiopia and hurting different land use types in the Horro district of the study area and fact, more research has not been done yet on the weed is highly infested and persisted for three decades. Therefore, this study was conducted to assess the weeds flora and seed bank status across different land use types in the Horro district of western Oromia, Ethiopia. Three locations and from each location five different land use were purposively selected and a 1m2 area of quadratic sampling techniques was used to record weed flora. From each quadrat, soil samples were taken having two soil depth levels (0-15cm and >15-30cm) to evaluate the soil seed bank of weeds. Results: The data of wild radish weed in different location and different land use types both field survey and greenhouse experiments were found a statistically significant difference (p<0.05) among the target action of land use types but there was found no significant difference among soil depths. More all less, the highest wild radish population was recorded from grazing land land followed by wheat crop land and among the locations, Gitilo Dale has the highest weed density. Among the weed flora, again wild radish was highly dominated weed species than the other weed species in all land use types and locations. From soil seed bank status of soil depth, high weed population was recorded at 0-16cm of second season weed emergence from pot and Gitilo Dale was the highest infected location at this season, while barley crop land was the highest infected among land use types in first season of weed emergence from pot. Among the weed species were recorded from soil depth, again wild radish was highly dominated in both seasons. The population of weed flora per quadrat was directly proportion with population recorded from soil depth that emerged from pot. Conclusions: This study has highly verified that this area has highly infected by wald radish (Raphanus raphanistrum) and there could be a source of weed seed. Generally, the concerned bodies should have to be given attention which should need immediate intervention strategies to mitigate the negative impacts of weed otherwise the area will be out of production soon. Therefore, an extensive integrated weed management strategies should be applied in all phases of cropping systems to sustain environment and reduce this weed in the future.
Quantifying Radial Growth Response of Pinus Yunnanensis to Climate Change and Drought Event at Different Altitudes and Ages in the Jinsha River Basin
Authors: Su Jianrong , Shen Jiayan , Li Shuaifeng , Huang Xiaobo , Liu Wande , Lang Xuedong
Published: June 2021

Background

The relative influence of climate change and drought events on tree growth at different altitude and tree ages remains insufficiently understood in the Jinsha River Basin, southwest China, limiting prediction of forest adaptability to high-frequency droughts and climate change. We conducted a dendroecological study to explore and quantify the dominant climate factors that determining radial growth of Pinus yunnanensis trees of different ages and at different altitudes, to evaluate their resilience to drought events.
Results

Radial growth of P. yunnanensis at high elevations is typically limited by low temperatures, the explanatory rate of temperature factors on growth increased from 23.6–59.7% with altitude. Tree growth at low elevations is more sensitive to moisture factors, the explanatory rate of moisture factors on growth decreased from 76.4–40.3% with altitude. The young and mature trees are more prone to moisture factors than middle-age and near-mature trees, the young and near-mature trees are more prone to temperature factors than middle-age and mature trees. The older trees usually showed less drought resistance and recovery than the young and middle-age trees. The resistance and recovery of P. yunnanensis weakened with the increased frequency of drought events. Tree resistance and resilience was highly dependent on the average pre-drought growth, whereas the recovery showed weak or no significant relationships with average pre-drought growth.
Conclusion

Our study demonstrates that radial growth of P. yunnanensis trees showed age- and altitude-specific demand for energy and moisture. P. yunnanensis trees at different altitudes and ages are differentially adapted to varying levels of climate stress and display different strategies to withstand the effects of drought with altitude and ages.
Integrated Analysis of Tumor‑Associated Macrophage Infiltration and Prognosis in Ovarian Cancer
Authors: tan qianxia , liu huining , xu jie , mo yanqun , wu chenchun , dai furong
Published: June 2021

BackgroundMost ovarian cancer patients with poor prognosis and immune microenvironment play a vital role in the progression of ovarian cancer. We aim to develop a tumor-associated macrophage related gene (TAMRGs) prognostic signature that can stratify and predict overall survival for ovarian cancer.MethodsWe acquired single cell and bulk transcriptome raw data of ovarian cancer from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). The immune landscape was identified in primary and ascites of ovarian cancer. CIBERSORT deconvolution algorithm, Weighted gene co-expression network analysis (WGCNA), univariate cox analysis, LASSO algorithm, and multivariate cox analysis were performed for the identification of TAMRG and the development of prognostic signature.Results We identified inter-and intra-patient heterogeneity for immune infiltration cells at a single-cell resolution. Tumor infiltration macrophages showed immunosuppressive characteristics with an M2 phenotype. T cell CD4 memory activated, mast cell activated, neutrophils and macrophages M2 were negatively correlated with overall survival, while macrophages M1 was positively correlated. A total of 219 TAMRGs were identified and a novel 6-gene signature (TAP1, CD163, VSIG4, IGKV4-1, CD3E, and MS4A7) with superior prognostic independence was established.ConclusionsThe TAMRG-based signature is expected to be a promising target for prognosis and treatment response of ovarian cancer.
Ketamine used in the therapy of depressive disorders impacts protein profile, proliferation rate and phagocytosis resistance of enterococci
Authors: Jarzembowski Tomasz Artur , Daca Agnieszka , Cubała Wiesław J. , Bronk Marek , Naumiuk Łukasz
Published: June 2021

Background: Ketamine is known to cause rapid anti-depressive effect. Additionally, it has been also proved that at high concentrations ketamine inhibits bacterial growth. It is also widely known that even sub-inhibitory concentration of chemicals, as concentration of ketamine used in therapy of depression, may change bacterial properties, including their virulence. The knowledge about possible influence of ketamine on bacterial commensals seems to be essential, as the mechanism of ketamine’s action in depression is believed to result also from its’ anti-inflammatory activity. In the current study we aimed to evaluate the in vitro influence of ketamine on proliferation rate, phagocytosis resistance and toxicity of enterococci. Results: The studied enterococcal strains were isolated as etiological agents of infection and collected in the Department of Medical Microbiology, Medical University of Gdansk. To measure metabolic activity of Enterococcus faecalis 10µM of CFDA-SE was added to bacterial suspension. The number of bacterial cells and fluorescence of particles were determined using FACSVerse flow cytometer. Additionally, for the determination of phagocytosis resistance, THP-1 human monocytes cell line from ATCC was used. Suspension of monocytes which engulfed bacteria was then stained with propidium iodide to determine cells’ membrane permeability and to evaluate cytotoxicity of enterococci. The result of the study proved diverse influence of therapeutic concentration of ketamine on Enterococci. In 23.1% of strains both proliferation rate and metabolism activity were inhibited. This group of strains was more susceptible to phagocytosis and had lower cytotoxicity than in culture without ketamine. Different response of isolates to ketamine was also visible in changes of protein profile determined by MALDI TOF. Conclusions: The analysis of bacteria at early stage of growth curve demonstrate the bacterial diversity in response to ketamine and let us set the hypothesis that microbiome susceptibility to ketamine may be one of the elements which should be taken into consideration when planning the successful pharmacotherapy of depression.
An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multi-material bioprinting
Authors: Engberg Adam , Stelzl Christina , Eriksson Olle , O'Callaghan Paul , Kreuger Johan
Published: June 2021

Bioprinting is increasingly used to create complex tissue constructs for an array of research applications, and there are also increasing efforts to print tissues for transplantation. Bioprinting may also prove valuable in the context of drug screening for personalized medicine for treatment of diseases such as cancer. However, the rapidly expanding bioprinting research field is currently limited by access to bioprinters. To increase the availability of bioprinting technologies we present here an open source extrusion bioprinter based on the E3D motion system and tool changer to enable high-resolution multi-material bioprinting. As proof of concept, the bioprinter is used to create collagen constructs using freeform reversible embedding of suspended hydrogels (FRESH) methodology, as well as multi-material constructs composed of distinct sections of laminin and collagen. Data is presented demonstrating that the bioprinted constructs support growth of cells either seeded onto printed constructs or included in the bioink prior to bioprinting. This open source bioprinter is easily adapted for different bioprinting applications, and additional tools can be incorporated to increase the capabilities of the system.
Identification of Fusarium commune, the Causal Agent of Postharvest Zinnia Meltdown Disease in Tennessee
Authors: Bika Ravi , Baysal-Gurel Fulya
Published: June 2021

The cut flower growers of the eastern and southern United States are threatened with postharvest meltdown of zinnia (Zinnia elegans), which reduces yield and income as well as limiting opportunities for production expansion. Disease symptoms such as bending of the stem just below the flower were visually apparent on zinnia cut flowers. The objective of this study was to identify the causal agent related to zinnia meltdown. A total of 20 symptomatic zinnia cut flower stems were collected from Tennessee. Several Fusarium-like colonies with micro and macroconidia were isolated from the base and bend area of stems on potato dextrose agar (PDA) and Fusarium-selective media. Morphological characterization, polymerase chain reaction, and sequencing of three representative isolates, FBG2020_198, FBG2020_199, and FBG2020_201, were conducted to confirm pathogen identification. The sequence identity of the isolates was >99% identical to Fusarium commune, and a combined phylogenetic tree grouped the isolates with the clade of F. commune from different host and geographical locations. To accomplish Koch’s postulates, a pathogenicity test was performed on ‘Benary’s Giant Golden Yellow’, ‘Benary’s Giant Lime’, and ‘Benary’s Giant Pink’ zinnia plants at vegetative (2 weeks after transplantation) or flower bud stage (1 month after transplantation) by drench, stem injection, and foliar spray of conidial suspension (1 × 105 conidia/mL). Similar symptoms of meltdown (floral axis bending just below the flower) were observed on inoculated zinnia cultivars 2 days after harvesting. Fusarium commune was re-isolated from the infected flower stems of all three cultivars but not from the noninoculated zinnia flower stems. Zinnia stem colonization by F. commune was statistically similar in all three tested cultivars regardless of plant growth stage and method of inoculation. This study confirms F. commune as being the causal agent of postharvest zinnia flower meltdown issue in Tennessee. In the future, possible sources of pathogen will be screened, and disease management recommendations will be developed.
Robust Subspace Tracking Algorithms in Signal Processing: A Brief Survey
Authors: Trung Thanh Le , Viet Dung Nguyen , Linh Trung Nguyen , Abed-Meraim Karim
Published: June 2021

Principal component analysis (PCA) and subspace estimation (SE) are popular data analysis tools and used in a wide range of applications. The main interest in PCA/SE is for dimensionality reduction and low-rank approximation purposes. The emergence of big data streams have led to several essential issues for performing PCA/SE. Among them are (i) the size of such data streams increases over time, (ii) the underlying models may be time-dependent, and (iii) problem of dealing with the uncertainty and incompleteness in data. A robust variant of PCA/SE for such data streams, namely robust online PCA or robust subspace tracking (RST), has been introduced as a good alternative. The main goal of this paper is to provide a brief survey on recent RST algorithms in signal processing. Particularly, we begin this survey by introducing the basic ideas of the RST problem. Then, different aspects of RST are reviewed with respect to different kinds of non-Gaussian noises and sparse constraints. Our own contributions on this topic are also highlighted.
La mente autoritaria: de las metáforas políticas a la constitución de la identidad social
Authors: González Sebastián Alejandro , Garativo María Clara
Published: June 2021

Este artículo trata sobre los procesos mentales de las personas autoritarias. En primer lugar, abordamos tales procesos desde el tipo de metáforas que encarnan, al entender las metáforas como marcos conceptuales que configuran el pensamiento humano. Esos marcos se entienden como constructos sociales de los que se apropia el pensamiento individual del ser social. En ese orden de ideas, indagamos por los marcos de referencia a los que apela el pensamiento autoritario. Uno de ellos es el marco de la familia y la del padre como figura principal que explica las relaciones jerárquicas. Otro es el dualismo amigo-enemigo: a través de éstos, la identidad autoritaria establece unas relaciones con los otros que corresponden a una visión maniquea del mundo: los amigos serán aquellos que son capturados por las metáforas a las que apela el autoritario y su forma particular de entender el mundo; los otros son los enemigos. Así, el autoritario se concibe como un héroe, con lo que esta figura (como marco conceptual en sí misma) permite entender ciertos imaginarios y su relación con un objetivo personal: tal objetivo deja de ser individual para ser la meta del colectivo, y el autoritario se autoproclama como el garante de su consecución. Finalmente, desde los marcos como constructos sociales pasamos a indagar acerca del carácter del autoritario como síntoma de una particular constitución de identidad; tal constitución nos recuerda la figura del psicópata exitoso, cuya identidad está formada sobre por unas agendas y una perspectiva del mundo inflexibles que incluso ponen en riesgo su propia integridad.
The Suffering of Invertebrates: An Approach from Animal Ethics
Authors: Villamor Iglesias Alejandro
Published: June 2021

Invertebrate animals are usually seen as a kind of “aliens” which do not deserve any moral consideration. However, there is a growing amount of evidence indicating that many of them do have the capacity to experience pain. The same criteria that are usually applied in order to infer that vertebrates are sentient beings (behavioral response, learning capacity, memory, a certain specific neurophysiological structure…) lead to the idea that many invertebrates are sentient as well. Therefore, under the skeptical premise that we have no direct evidence of the experience of pain in vertebrates, we are forced to hold that it exists in both vertebrates and invertebrates.
Imagen y diferencia. Una aproximación deconstructiva a la teoría de la diferencia icónica de Gottfried Boehm
Authors: Rubio Roberto , Vignola Jacopo
Published: June 2021

Con su teoría acerca de la diferencia icónica, Boehm ha enfatizado el carácter intrínsecamente diferencial de la imagen. Esto ha llevado a algunos investigadores (especialmente Dieter Mersch) a poner en relación el enfoque de Boehm con el pensamiento de Jacques Derrida. Profundizando en esa línea interpretativa, proponemos una aproximación deconstructiva a la teoría de Boehm acerca de la diferencia icónica. Esto significa, en concreto, que sacaremos a luz tensiones internas en la propuesta de Boehm entre énfasis dirigidos hacia el carácter diferencial de la imagen, por una parte, y planteos dualistas y distinciones jerárquicas que podrían encubrir tal carácter, por otra parte. A partir de esas tensiones, intentaremos radicalizar la orientación hacia la estructura diferencial de la imagen incorporando planteos de Derrida, pero dejando en claro a la vez los desafíos que ello traería para la propuesta de Boehm y las cuestiones abiertas que surgirían de allí.
Integration of Statistical Models and Computer Simulation in Environmental Accidents: A Study on Leakage of Red Mud in the Pará River, Amazon, Brazil
Authors: de Sousa Jr. Pedro , Pereira Simone , Rocha Ronaldo , Alves Mateus , Pinheiro Luciana , Nogueira Daniel , Teixeira Orivan , Gonçalves Auriane
Published: June 2021

The introduction of potentially toxic elements (PTEs) from industrial activities in water bodies, highlights the vulnerability of the environment and the need for actions focused on the implementation of mechanisms that help in the control and remediation of these impacts. The objective of the study was based on the integration of instrumental analytical methods, multivariate statistics, and computer simulation models, with the proposal of implementing realistic scenarios of the red mud plume behavior in the Pará River. The selected location was the municipality of Barcarena-PA, where is located an important alumina/aluminum industrial center. With the aid of the inductively coupled plasma optical emission spectrometer (ICP OES), the chemical elements Al, B, Cr, Fe, Mn, P, Ti, and Zn were quantified and the principal component analysis (PCA) allowed to select Al, B, Fe, P, and Zn as the most relevant with 98.68% of the accumulated variance. Some scenarios were proposed by the simulated hydrodynamic model, which evidenced that the Trambioca community would be the most affected in the first 13 h after the effluent overflowed, and the other locations in domino effect. The main scientific gain of the proposal is the possibility of the predictive scenarios to help the actions of containment and mitigation of environmental damages by public control agencies, institutions involved, and other competent bodies.
Optimization of the electrochemical oxidation of textile wastewater by graphite electrodes by response surface methodology and artificial neural network
Authors: Saleh Mohammed , Yildirim Rabia , Isik Zelal , Karagunduz Ahmet , Keskinler Bulent , Dizge Nadir
Published: June 2021

In this study, electrochemical oxidation of combed fabric dyeing wastewater was investigated using graphite electrodes. The response surface methodology (RSM) was used to design the experiments via the central composite design (CCD). The planned experiments were done to track color changes and chemical oxygen demand (COD) removal. The experimental results were used to develop optimization models using RSM and the artificial neural network (ANN) and they were compared. The developed models by the two methods were in good agreement with the experimental results. The optimum conditions were found at 150 A/m2, pH 5, and 120 min. The removal efficiencies for color and COD reached 96.6% and 77.69%, respectively. The operating cost at the optimum conditions was also estimated. The energy and the cost of 1 m3 of wastewater required 34.9 kWh and 2.58 US$, respectively. The graphite electrodes can be successfully utilized for treatment of combed fabric dyeing wastewater with reasonable cost.
PERFIL EPIDEMIOLÓGICO DE ACIDENTES COM ANIMAIS PEÇONHENTOS NO ESTADO DO MARANHÃO
Authors: Cordeiro Eduardo Costa , Almeida Joelson dos Santos , Da Silva Thiago Sousa
Published: June 2021

Introdução: Considerados como um problema de saúde pública, os acidentes causados por animais peçonhentos (ofídios, escorpiões, aranhas e dentre outros) são responsáveis por um considerável número de agravos à população mundial. Na região avaliada, estudos acerca do tema são inexistentes, havendo a necessidade do levantamento de dados, uma vez que, informações atualizadas são de fundamental importância para que ações de vigilância epidemiológica sejam empregadas com maior segurança. Objetivo: Descrever o perfil epidemiológico dos acidentes com animais peçonhentos no estado do Maranhão. Metodologia: Trata-se de um estudo ecológico, descritivo com abordagem quantitativa. Os dados foram coletados no SINAN/DATASUS dos casos notificados de acidentes com animais peçonhentos. Resultados: Constou-se uma maior incidência de notificações de acidentes em 2019 e nos meses de Fevereiro (10,14%), Março (9,96%) e Maio (9,89%). No que tange ao perfil das vítimas houve uma predominância no sexo masculino (68,16%), adultos jovens de 20 a 39 anos (36,24%) e autodeclarados pretos (75,03%).  No que diz respeito à gestação a imensa maioria não se aplica a categoria gestante (77,71%). Em relação às características do acidente destacou-se o acidente escorpiônico (55,97%), seguido pelos acidentes com aranhas (30,66%) e ofídicos (4,09%). O tempo decorrido entre o acidente e o atendimento foi predominante no intervalo de 1 a 3 h após o acidente (34,37%). Dos acidentes ocorridos 61,31% foram classificados como leves, 82,02% evoluíram clinicamente com cura e 0,6% para óbito. Conclusão: Com base nos dados apresentados, torna-se evidente a importância dos acidentes causados por animais peçonhentos para a saúde pública, assim aponta-se que medidas intersetoriais entre a vigilância epidemiológica, a secretaria do meio ambiente e o centro de controle de zoonoses são essenciais para a diminuição da mortalidade por acidentes com animais venenosos.
A CONTRIBUIÇÃO DO PROJETO “INFOJOVEM” NA EDUCAÇÃO EM SAÚDE DE ADOLESCENTES
Authors: Bomfim Ana Marlusia Alves , Leão Melro Ana Flávia
Published: June 2021

Introdução: A adolescência é um período do ciclo da vida balizado pela influência da vulnerabilidade, onde o adolescente passa por fases de crescimento e desenvolvimento no âmbito físico e intelectual, e merece uma atenção especial por parte de todos aqueles que lidam com o mesmo. Objetivo: Descrever uma experiência vivenciada por discentes e docentes, na participação de um projeto de extensão sobre a prevenção de saúde dos adolescentes. Metodologia: Trata-se de um estudo descritivo, tipo relato de experiência, acerca das atividades realizadas por discentes e docentes do curso de medicina do Centro Universitário Tiradentes- UNIT/AL, entre os meses de abril e junho de 2019, com adolescentes de ambos os sexos e faixa etária entre 12 e 18 anos, residentes em bairros da periferia de Maceió. Foi utilizada como técnica a roda de conversa e recursos audiovisuais, como facilitadores do processo da aprendizagem. Resultados: As atividades experiênciadas possibilitaram a formação de vínculos, facilitando a integração do público alvo à proposta metodológica utilizada durante os encontros. Assim, percebe-se que houve um processo de aprendizagem profícuo, onde os adolescentes se sentiram entusiasmados a serem protagonistas na vivência dos encontros realizados sobre a temática em tela. Conclusões: A prática educativa com adolescentes provenientes de uma conjuntura social vulnerável, oportunizou aos discentes e docentes reflexões da agradável tarefa de serem facilitadores do conhecimento. Além de perceberem que é um desafio lidar com um grupo que se encontra em formação e que possui diversas indagações e conflitos pertinentes a própria idade.

 

Palavras-Chave: educação em saúde; saúde do adolescente; saúde da comunidade.
Anais do 1° Congresso Online de Ortodontia
Authors: Fuziy Acácio , De Araújo Gurgel Júlio
Published: June 2021

A pandemia do COVID-2019 obrigou o cancelamento de diversos eventos das mais diversas áreas de pesquisa, inclusive da Ortodontia. Portanto, observou-se a ocorrência da restrição para divulgação da relevante produção científica da Ortodontia brasileira. Assim sendo, pensou-se na possibilidade da realização de um evento científico para continuar com a propagação dos resultados das pesquisas e informações obtidas em estudos clínicos e laboratoriais e, desta forma, contribuir com a Ciência da Ortodontia Brasileira e da América do Sul. Em um período de 3 dias, o 1° Congresso Online de Ortodontia contou com a participação de palestrantes internacionais e de professores brasileiros das mais diversas regiões. Os alunos dos Programas de Pós-graduações puderam participar, apresentando as pesquisas e relatos de casos clínicos, contribuindo com a Especialidade. Ao mesmo tempo a oportunidade de apresentar trabalhos propiciou o estímulo aos pós-graduandos que tiveram a oportunidade de valorizar a pesquisa ortodôntica.
AÇÕES DO ENFERMEIRO NA PREVENÇÃO DE INFECÇÕES ASSOCIADAS AO USO DE CATETERES EM UNIDADE DE TERAPIA INTENSIVA: REVISÃO INTEGRATIVA
Authors: Teixeira Rayssa , Da Silva Miriane Virlane , Da Silva Edla Maria , Neves Santos Ruth Stephanie , Souza Vanderley Érica Catia , De Carvalho Silva Cintia , Lopes Oliveira Diego Augusto
Published: June 2021

Introdução: As infecções relacionadas ao uso de cateteres na Unidade de Terapia Intensiva (UTI) representam uma realidade de altos índices, que consolidam-se como problema de saúde pública e sobre o qual a equipe de enfermagem se caracteriza como potencial agente transformador a partir do estabelecimento de medidas específicas de cuidados com esses dispositivos. Objetivo: Elucidar as ações do enfermeiro na prevenção de infecções associadas ao uso de cateteres na UTI. Metodologia: Estudo descritivo do tipo revisão integrativa realizado nos anos de 2015 a 2019, no período de março a maio nas bases de dados MEDLINE, LILACS e BDENF. Construído a partir de 6 etapas. A análise dos estudos se deu pela leitura de títulos, resumos, e artigos completos, uma vez que estes respondessem o objetivo determinado. Posteriormente a síntese dos resultados encontrados foi apresentada em tabelas, sendo seguida pela discussão a partir de categorias temáticas identificadas através da Técnica de Análise de Conteúdo. Resultados: Através dos estudos selecionados, observou-se uma gama de boas práticas que podem ser utilizadas pela enfermagem na garantia da qualidade do cuidado com os dispositivos, sendo elas referentes aos aspectos técnicos do manuseio ou não, como higiene das mãos, uso de EPI’s, dupla checagem, estratégias de desinfecção, boa comunicação entre profissionais e métodos para trabalhar fatores interferentes no manejo dos cateteres, como estresse, insegurança e esgotamento. Conclusões: Sugere-se a intensificação da padronização de bundles e protocolos de medidas, bem como melhoria na abrangência destes, para que considerem às várias etapas de manejo com os cateteres. Percebe-se a necessidade de entender um conjunto de fatores que interferem na adesão e disseminação das boas práticas de enfermagem nesse contexto. Enfatiza-se a necessidade de estudos que detalhem de modo aprofundado a realização das boas práticas, e discutam fatores que afetam o sucesso da implantação das mesmas.

Palavras-Chave: Infecções Relacionadas a Cateter; Unidades de Terapia Intensiva; Cuidados de Enfermagem.
INFLUÊNCIA DA TÉCNICA DE PREPARO SOBRE O CONTORNO CERVICAL DOS LAMINADOS CERÂMICOS: RELATO DE CASO
Authors: Luiza Carvalho da Silva Lívia , Ferreira da Silva Douglas , Lins Fernanda Carvalho de Rezende , Farias Rodrigues Raphaela
Published: June 2021

Introdução: Devido à crescente demanda estética, a odontologia restauradora se renova na intenção de alcançar melhores resultados na harmonia do sorriso. Entretanto, a atenção deve voltar-se também aos tecidos periodontais, visto que há uma integração entre material restaurador e os tecidos. Objetivo: Descrever e discutir as etapas clínicas para a confecção de laminados cerâmicos, atentando-se a preparos minimamente invasivos, contorno cervical e fatores que podem intervir na longevidade do procedimento. Relato de caso: Paciente do sexo masculino, 28 anos de idade, compareceu a Associação Brasileira de Odontologia – ABO, seção de Alagoas, relatando insatisfação em relação ao tamanho dos seus dentes. Os arcos superior e inferior foram moldados para a obtenção dos modelos de estudo e enceramento diagnóstico. Ao início do tratamento foi orientado ao paciente clareamento dental pela técnica combinada. O preparo dos dentes foi guiado por uma matriz de silicone, sendo estes uniformes e conservadores, seguido de moldagem, fase laboratorial e cimentação final. Finalizado o caso foi realizado o ajuste oclusal solicitando ao paciente que realizasse movimentos de lateralidade e protrusão. Conclusões: A técnica do preparo influência na longevidade dos laminados cerâmicos, por relacioanar-se à adesão e a saúde periodontal. O preparo deve ser mínino e limitado ao esmalte dental sempre que possível, determinando assim um maior e melhor prognóstico.
APRENDIZADOS E REFLEXÕES ADVINDOS DE ATIVIDADE EXTENSIONISTA DE EDUCAÇÃO EM SAÚDE EM CENTROS DE EDUCAÇÃO INFANTIL
Authors: Oliveira Tomaz Ferraresso Lucas Fernando , Britto Codato Lucimar Aparecida
Published: June 2021

Introdução: Atividade extensionista configura-se como poderoso instrumento para a formação integral e contextualizada do estudante. Favorece agregação, aplicação e absorção de múltiplos conhecimentos na comunidade. O projeto Ações de Educação em Saúde e de Educação Continuada para Profissionais do SUS da Universidade Estadual de Londrina, desenvolve atividades de educação em saúde vinculadas às demandas apresentadas pelos Centros de Educação Infantil que atua. Objetivos:  Relatar o processo ensino-aprendizagem e os desafios vivenciados em uma atividade extensionista e correlacioná-los com a curricularização da extensão. Metodologia: Trata-se de um estudo descritivo do tipo relato de experiência extensionista, desenvolvido por estudantes do terceiro ao décimo período e professores de Odontologia. O estudo refere-se ao período de 2018 e 2019. Resultados: Foram realizadas ações de educação em saúde sobre adoção de hábitos saudáveis relativos à saúde geral das crianças, pais e cuidadores por meio da elaboração e distribuição de folders. Para os integrantes do projeto, esta demanda foi desafiadora em relação à definição de estratégias, planejamento, conciliação de horários e adoção de recursos que favorecessem a comunicação com a população. Esta experiência extensionista, remeteu à reflexão sobre a amplitude das potencialidades da curricularização da extensão,  porque alia a relevância social e favorece a formação integral do estudante. Porém, o alcance dos objetivos das ações extensionistas e da curricularização da extensão, dependem do envolvimento e dedicação dos docentes e estudantes. Conclusões: Atividades extensionistas e a curricularização da extensão são relevantes para o ensino-aprendizagem e para cumprimento do compromisso social das universidades. São complexas e multifacetadas, para além dos projetos apresentados e da implementação de legislações e diretrizes educacionais.

Palavras-Chave: Relações Comunidade-Instituição; Educação Superior; Educação em Saúde.
CONTRIBUIÇÕES DA VIVÊNCIA EM COMUNIDADE PARA FORMAÇÃO ACADÊMICA EM FISIOTERAPIA
Authors: Néry do Nascimento Arlon , Brito da Silva Amanda Maria , Carvalho Caxias Carolyne , Alves de Paula Dionatan Christophe , Carneiro Alves Pereira Mayane
Published: June 2021

Introdução: Durante sua formação o fisioterapeuta é preparado para atuar em todosos níveis de atenção, não ficando restritos somente às ações reabilitadoras e curativasàs quais são comumente associados, a promoção e educação em saúde, faz parte desuas atribuições e visam a autonomia na saúde da população. Objetivo: Descrever apercepção dos acadêmicos de fisioterapia sobre a vivência no estágio supervisionadono setor de saúde da comunidade. Metodologia: Os relatos apresentados nesteestudo estão vinculados à atuação de quatro discentes do curso de Fisioterapia emuma Unidade Básica de Saúde do município de Piripiri-PI, durante o período defevereiro a março do ano de 2020. As atividades eram realizadas três vezes porsemana, ficando estabelecido a divisão em três momentos (Alongamento e aferiçãoda pressão arterial; Dinâmica; Roda de conversa), com o intuito de trazer umamelhor interação da comunidade com os acadêmicos, proporcionar ganhos de saúdeaos participantes e promover um maior conhecimento sobre os temas abordados.Resultados e discussão: Essas ações realizadas durante o estágio, resultaram naconstrução de um vínculo entre os acadêmicos e a comunidade gerando promoção eeducação para ambos os lados, bem como a desconstrução da sociedade sobre asatribuições do fisioterapeuta. Conclusões: Desta forma finda-se que o estágio nacomunidade agrega ao conhecimento teórico e prático dos acadêmicos umaexperiência rica, que leva ao desenvolvimento de habilidades e trabalho em equipe,assim como proporciona uma maior valorização sobre a importância da atençãoprimária e o papel do fisioterapeuta na equipe multidisciplinar.
AVALIAÇÃO EPIDEMIOLÓGICA DE PACIENTES COM CÂNCER DE BOCA E OROFARINGE DA LIGA MOSSOROENSE DE ESTUDOS E COMBATE AO CÂNCER
Authors: Maria Gadelha de Medeiros Alana , Alexandre de Sousa Canuto Juney , Aires Mendonça Fonseca Denner , Adson Costa Coelho Wesley , Lopes Cavalcanti Raquel
Published: June 2021

Introdução: Os tumores de cabeça e pescoço, têm expressiva incidência e mortalidade, assim como alta letalidade, e constituem um relevante problema de saúde pública, particularmente nos países em desenvolvimento. Dentre as neoplasias malignas diagnosticadas no mundo, aproximadamente 10% estão localizadas na boca, sendo esse o sexto tipo de câncer mais incidente. Objetivo: Avaliar o perfil epidemiológico de pacientes diagnosticados com câncer de boca e faringe da Liga Mossoroense de Estudos e Combate ao Câncer do município de Mossoró, RN, entre janeiro 2013 e junho de 2018. Metodologia: Estudo transversal, fundamentado na análise retrospectiva e descritiva de prontuários médicos. Resultados: Dos 221 prontuários analisados, a cor branca (56,6%) e o gênero masculino (70,6%) foram os mais prevalentes, com média de idade entre 55 e 65 anos. A maioria (61,9%) apresentava ensino fundamental incompleto, sendo residentes de área urbana (59,6%) com histórico de uso de tabaco (64,6%) e/ou bebidas alcoólicas (53,9%). O sítio mais prevalente de câncer em boca foi a base da língua (12,7%). Conclusões: Conhecer o perfil dos pacientes com câncer de boca e orofaringe é um importante passo para melhor traçar e direcionar ações de saúde pública visando tanto à prevenção quanto o diagnóstico precoce.
Thymic Immunosuppressive Pentapeptide (TIPP) Shown Anticancer Activity in Breast Cancer and Chronic Myeloid Leukemia Both In Vitro and In Vivo
Authors: Ijaz Muhammad , Shahbaz Muhammad , Jiang Wenjie , Shi Yikang , Guo Xiuli , Wang Fengshan
Published: June 2021

Aim:

Being the common cause and major burden of deaths globally, timely cancer management
is crucial.

Background:

Thymic immunosuppressive pentapeptide (TIPP) is a novel pentapeptide originally
obtained from calf thymic immunosuppressive extract. Previously, TIPP has been proved to suppress
the allergic and inflammatory responses in allergic mice via blocking MAP kinases/NF-κB
signaling pathways.

Objective:

In this study, in vitro anticancer activity of TIPP was tested on two different types of
cancers using MCF-7 and K562 cell lines.

Methods:

Tumor xenograft models for breast cancer and chronic myeloid leukemia were designed.
In vivo anticancer activity of TIPP was investigated on both cancer types. The liver and tumor tissues
of the mice were preserved for immunohistochemistry analysis.

Results:

In vitro anticancer activity of TIPP showed significant inhibition on cell viability of both
breast cancer and chronic myeloid leukemia. In vivo anticancer effect of TIPP in both types of cancer
models further proved the potent anticancer nature of TIPP. Immunohistochemistry analysis assured
that TIPP is a safe drug for normal organs such as the liver.

Conclusion:

Our present study revealed that TIPP is a potent anticancer drug and an important
treatment option for various diseases. Further work is needed to test the flexible and proficient activity
of the novel peptide.
Effect of Nitrate on Gene and Protein Expression of Nitric Oxide Synthase
Enzymes in Insulin-Sensitive Tissues of Type 2 Diabetic Male Rats
Authors: Shokri Majid , Jeddi Sajad , Faridnouri Hassan , Khorasani Vajiheh , Kashfi Khosrow , Ghasemi Asghar
Published: June 2021

Background and Objective:

Decreased nitric oxide (NO) bioavailability contributes to
the pathophysiology of type 2 diabetes mellitus (T2DM). This study aims to determine the effects
of nitrate (NO3
–) on gene and protein expression of NO synthase (NOS) enzymes in the liver,
soleus muscle (SM), and epididymal adipose tissue (eAT) of rats with T2DM.

Methods:

Twenty-eight male rats were divided into 4 groups: Control, diabetes, control+NO3
–, and
diabetes+NO3
– (n = 7/each group). NO3
– was administered for 6 months, and mRNA and protein
levels of NOS enzymes were measured at the end of the study.

Results:

mRNA and protein levels of inducible NOS (iNOS) were higher in the liver (475% and
73%), SM (271% and 43%), and eAT (543% and 24%) of rats with T2DM. In the case of the endothelial
NOS (eNOS), diabetic rats had lower mRNA and protein levels in the liver (26% and
24%) and SM (60% and 62%) and lower mRNA level (30%) in eAT. mRNA and protein levels of
neural NOS (nNOS) were lower in SM (69% and 73%) and eAT (25% and 31%) of rats with
T2DM. NO3
– administration restored disrupted iNOS and eNOS expressions to their near normal
values in all the studied tissues; NO3
– also increased nNOS mRNA and protein levels in SM and
eAT but decreased nNOS protein level in the liver.

Conclusion:

Long-term NO3
– administration restored disrupted expression of NOS enzymes in the
liver, SM, and eAT of rats with T2DM; these findings partly explain the beneficial metabolic effects
of nitrate in T2DM.