bluuebunny/crossref_metadata_2025 huggingface dataset:
Local Use of Hydrogel with Amiodarone in Cardiac Surgery: Experiment and Translation to the Clinic
Authors: Shvartz Vladimir , Kanametov Teymuraz , Sokolskaya Maria , Petrosyan Andrey , Le Tatyana , Bockeria Olga , Bockeria Leo
Published: March 2021

The objective of this study was to study the use of the hydrogel biopolymer based on sodium alginate (“Colegel”) with a drug substance—amiodarone—for the prevention of postoperative atrial fibrillation (POAF) in cardiac surgery. The experimental part of the study was performed on 46 rabbits. Five groups were formed: in the first group, the dose of amiodarone in hydrogel was 1 mg; in the second group—3 mg; in the third group—6 mg; in the fourth group, hydrogel was used without amiodarone; in the fifth group, 60 mg amiodarone was administered intravenously. The animals from each group were removed from the experiment for the pathomorphological study of the heart after 3, 7 and 14 days. The studied endpoints were: the heart rate control; the development of the blockades of the conduction system of the heart; and the development of inflammation according to laboratory pathomorphological studies. The translational clinical part involved a randomized clinical trial which included 60 patients, with an average age of 62 ± 8.5 years. All patients were randomized into two groups: the study group (n = 30, with the application of amiodarone hydrogel) and the control group (n = 30, without the application of amiodarone hydrogel). The dose of amiodarone in the hydrogel material was 60 mg for all patients. The heart rhythm was monitored during 5 days. The primary endpoint was the development of POAF. Secondary endpoints were: the dynamics of heart rate; the duration of the QT and PQ intervals; the development of blockades of the cardiac conduction system; as well as the dynamics of AST and ALT. According to the results of the experimental part, it was found that the method of the local epicardial delivery of amiodarone by the hydrogel material was safe. Hydrogel with amiodarone is effective for reducing the heart rate in the animal experiment in comparison to the control group and the group with the intravenous administration of the drug. The optimal dose of amiodarone in hydrogel was 1 mg per 1 kg. According to the results of the clinical part, it was found that the method of the local epicardial delivery of amiodarone as a hydrogel material proved its safety. Hydrogel with amiodarone at a dose of 60 mg was effective in preventing POAF in patients after coronary artery bypass grafting (CABG) operations in comparison to the control group (p < 0.001). The age and procedure of application of the amiodarone gel were significantly associated with POAF (p = 0.009 and p = 0.011, respectively). The use of hydrogel with amiodarone reduced the probability of developing POAF 18.9-fold. The method of the local epicardial delivery of amiodarone in the form of a hydrogel material is safe. The use of hydrogel with amiodarone after CABG reduced the probability of developing POAF.
Leptin Modulates the Response of Brown Adipose Tissue to Negative Energy Balance: Implication of the GH/IGF-I Axis
Authors: Barrios Vicente , Frago Laura M. , Canelles Sandra , Guerra-Cantera Santiago , Arilla-Ferreiro Eduardo , Chowen Julie A. , Argente Jesús
Published: March 2021

The growth hormone (GH)/insulin-like growth factor I (IGF-I) axis is involved in metabolic control. Malnutrition reduces IGF-I and modifies the thermogenic capacity of brown adipose tissue (BAT). Leptin has effects on the GH/IGF-I axis and the function of BAT, but its interaction with IGF-I and the mechanisms involved in the regulation of thermogenesis remains unknown. We studied the GH/IGF-I axis and activation of IGF-I-related signaling and metabolism related to BAT thermogenesis in chronic central leptin infused (L), pair-fed (PF), and control rats. Hypothalamic somatostatin mRNA levels were increased in PF and decreased in L, while pituitary GH mRNA was reduced in PF. Serum GH and IGF-I concentrations were decreased only in PF. In BAT, the association between suppressor of cytokine signaling 3 and the IGF-I receptor was reduced, and phosphorylation of the IGF-I receptor increased in the L group. Phosphorylation of Akt and cyclic AMP response element binding protein and glucose transporter 4 mRNA levels were increased in L and mRNA levels of uncoupling protein-1 (UCP-1) and enzymes involved in lipid anabolism reduced in PF. These results suggest that modifications in UCP-1 in BAT and changes in the GH/IGF-I axis induced by negative energy balance are dependent upon leptin levels.
Bacterial Nanocellulose toward Green Cosmetics: Recent Progresses and Challenges
Authors: Almeida Tânia , Silvestre Armando J. D. , Vilela Carla , Freire Carmen S. R.
Published: March 2021

In the skin care field, bacterial nanocellulose (BNC), a versatile polysaccharide produced by non-pathogenic acetic acid bacteria, has received increased attention as a promising candidate to replace synthetic polymers (e.g., nylon, polyethylene, polyacrylamides) commonly used in cosmetics. The applicability of BNC in cosmetics has been mainly investigated as a carrier of active ingredients or as a structuring agent of cosmetic formulations. However, with the sustainability issues that are underway in the highly innovative cosmetic industry and with the growth prospects for the market of bio-based products, a much more prominent role is envisioned for BNC in this field. Thus, this review provides a comprehensive overview of the most recent (last 5 years) and relevant developments and challenges in the research of BNC applied to cosmetic, aiming at inspiring future research to go beyond in the applicability of this exceptional biotechnological material in such a promising area.
Activin A and Cell-Surface GRP78 Are Novel Targetable RhoA Activators for Diabetic Kidney Disease
Authors: Soomro Asfia , Trink Jackie , O’Neil Kian , Li Renzhong , Naiel Safaa , Gao Bo , Ask Kjetil , Krepinsky Joan C.
Published: March 2021

Diabetic kidney disease (DKD) is the leading cause of kidney failure. RhoA/Rho-associated protein kinase (ROCK) signaling is a recognized mediator of its pathogenesis, largely through mediating the profibrotic response. While RhoA activation is not feasible due to the central role it plays in normal physiology, ROCK inhibition has been found to be effective in attenuating DKD in preclinical models. However, this has not been evaluated in clinical studies as of yet. Alternate means of inhibiting RhoA/ROCK signaling involve the identification of disease-specific activators. This report presents evidence showing the activation of RhoA/ROCK signaling both in vitro in glomerular mesangial cells and in vivo in diabetic kidneys by two recently described novel pathogenic mediators of fibrosis in DKD, activins and cell-surface GRP78. Neither are present in normal kidneys. Activin inhibition with follistatin and neutralization of cell-surface GRP78 using a specific antibody blocked RhoA activation in mesangial cells and in diabetic kidneys. These data identify two novel RhoA/ROCK activators in diabetic kidneys that can be evaluated for their efficacy in inhibiting the progression of DKD.
Co-Operativity between MYC and BCL-2 Pro-Survival Proteins in Cancer
Authors: Fairlie Walter Douglas , Lee Erinna F.
Published: March 2021

B-Cell Lymphoma 2 (BCL-2), c-MYC and related proteins are arguably amongst the most widely studied in all of biology. Every year there are thousands of papers reporting on different aspects of their biochemistry, cellular and physiological mechanisms and functions. This plethora of literature can be attributed to both proteins playing essential roles in the normal functioning of a cell, and by extension a whole organism, but also due to their central role in disease, most notably, cancer. Many cancers arise due to genetic lesions resulting in deregulation of both proteins, and indeed the development and survival of tumours is often dependent on co-operativity between these protein families. In this review we will discuss the individual roles of both proteins in cancer, describe cancers where co-operativity between them has been well-characterised and finally, some strategies to target these proteins therapeutically.
The Landscape of RNA-Protein Interactions in Plants: Approaches and Current Status
Authors: Burjoski Vesper , Reddy Anireddy S. N.
Published: March 2021

RNAs transmit information from DNA to encode proteins that perform all cellular processes and regulate gene expression in multiple ways. From the time of synthesis to degradation, RNA molecules are associated with proteins called RNA-binding proteins (RBPs). The RBPs play diverse roles in many aspects of gene expression including pre-mRNA processing and post-transcriptional and translational regulation. In the last decade, the application of modern techniques to identify RNA–protein interactions with individual proteins, RNAs, and the whole transcriptome has led to the discovery of a hidden landscape of these interactions in plants. Global approaches such as RNA interactome capture (RIC) to identify proteins that bind protein-coding transcripts have led to the identification of close to 2000 putative RBPs in plants. Interestingly, many of these were found to be metabolic enzymes with no known canonical RNA-binding domains. Here, we review the methods used to analyze RNA–protein interactions in plants thus far and highlight the understanding of plant RNA–protein interactions these techniques have provided us. We also review some recent protein-centric, RNA-centric, and global approaches developed with non-plant systems and discuss their potential application to plants. We also provide an overview of results from classical studies of RNA–protein interaction in plants and discuss the significance of the increasingly evident ubiquity of RNA–protein interactions for the study of gene regulation and RNA biology in plants.
Potential Enhancement of Metformin Hydrochloride in Lipid Vesicles Targeting Therapeutic Efficacy in Diabetic Treatment
Authors: Ossai Emmanuel Chekwube , Madueke Augustine Chidi , Amadi Benjamin Emenike , Ogugofor Martins Obinna , Momoh Audu Mumuni , Okpala Charles Odilichukwu R. , Anosike Chioma Assumpta , Njoku Obioma Uzoma
Published: March 2021

The potential enhancement of metformin hydrochloride (MH) loaded in lipid vesicles targeting therapeutic efficacy on alloxan-induced diabetic rats was investigated. This involved lipid vesicles formulated with homogenously distributed nano-sized particles by a novel integrated process of multiple emulsification by membrane and solvent evaporation. The average diameter of the water-in-oil (W1/O), W1/O/W2 emulsion droplets, and lipid vesicles was 192 nm, 52 µm, and 173 nm, respectively. The entrapment yield of metformin hydrochloride (MH) in the prepared lipid vesicles was 40.12%. The metformin hydrochloride-loaded lipid vesicles (MH-LLVs) sustained the release of the entrapped drug over a 12-h period and reduced the plasma glucose level of diabetic rats by 77.4% compared with free MH solution (2-h period and 58.2%, respectively) after one week post-diabetic treatment through oral administration of MH-LLV and the free drug. The remarkable improvement in the biochemical parameters recorded in the MH-LLV-treated animals compared with those that received free MH solutions depicted an enhanced kidney function, liver function, as well as oxidative stress status. Pancreatic histology depicted a pancreas with intralobular ducts (ID) and exocrine secretory acini that characterize an intact pancreas, which suggests the ability of the MH-LLVs to restore pancreatic cells to normal, on a continued treatment. Overall, MH-LLV appears an encouraging extended-release formulation with enhanced bioavailability, sustained release, and improved antihyperglycemic potentials.
Efficient Protection Scheme Based on Y-Source Circuit Breaker in Bi-Directional Zones for MVDC Micro-Grids
Authors: Al-khafaf Haider , Asumadu Johnson
Published: March 2021

A new bi-directional circuit breaker is presented for medium-voltage dc (MVDC) systems. The Y-source impedance network topology is used to implement the breaker. The current transfer function is derived to show the frequency response and the breaker operation with the high frequencies. Mathematical analysis is achieved with different conditions of coupling among the breaker inductors. The minimum level of the magnetic coupling is determined, which is represented by the null condition. The effect of the turns-ratio on this condition is investigated as well. The breaker is designed with two types of fault conductance slope rates. The Y-source breaker is simulated, and the results verify the breaker operation during the fault condition and the load change. The results also demonstrate the effect of the coupling level on the minimum values of the source current when the fault occurs. Based on the expected fault type in the MVDC systems, the proposed breaker is developed to interrupt the overcurrent due to any of these fault types. A protection scheme is proposed for a 12-bus, two-level micro-grid, where the Y-source breakers are used in the bi-directional zones. The results verify the ability of the breaker to conduct and interrupt the current in both directions of the power flow.
Volatility Co-Movement in Stock Markets
Authors: López-García María Nieves , Sánchez-Granero Miguel Angel , Trinidad-Segovia Juan Evangelista , Puertas Antonio Manuel , De las Nieves Francisco Javier
Published: March 2021

The volatility and log-price collective movements among stocks of a given market are studied in this work using co-movement functions inspired by similar functions in the physics of many-body systems, where the collective motions are a signal of structural rearrangement. This methodology is aimed to identify the cause of coherent changes in volatility or price. The function is calculated using the product of the variations in volatility (or price) of a pair of stocks, averaged over all pair particles. In addition to the global volatility co-movement, its distribution according to the volatility of the stocks is also studied. We find that stocks with similar volatility tend to have a greater co-movement than stocks with dissimilar volatility, with a general decrease in co-movement with increasing volatility. On the other hand, when the average volatility (or log-price) is subtracted from the stock volatility (or log-price), the co-movement decreases notably and becomes almost zero. This result, interpreted within the background of many body physics, allows us to identify the index motion as the main source for the co-movement. Finally, we confirm that during crisis periods, the volatility and log-price co-movement are much higher than in calmer periods.
Clinical Profile and Length of Hospital Stay in a Sample of Psychogeriatric Patients Referred to Consultation Liaison Psychiatric Unit
Authors: Barra Bernardo J. , Varela Luis F. , Maldonado José R. , Calvo Pilar , Bastidas Anna , Sánchez Roberto , Pintor Luis
Published: March 2021

Background and objectives: There has been a recent increase in older patients admitted to general hospitals. A significant percentage of hospitalized older patients are ≥75 years old, which differ from the patients aged 65 to 74 years old in terms of functional status at patient discharge. This study aims to compare sociodemographic, clinical features, and factors associated with length of hospital stay in youngest-old and oldest-old populations of inpatients referred to the consultation liaison psychiatry unit. Material and methods: This is an observational, cross-sectional, retrospective, and comparative study. We obtained data from a sample of 1017 patients (≥65 years) admitted to a general hospital and referred from different services (medicine, surgery, etc.) to the consultation liaison psychiatry unit. The sample was divided into two groups of patients: youngest-old (65–74 years) and oldest-old (≥75 years). Psychiatric evaluations were performed while the patients were on wards at the hospital. Psychopharmacs were started as needed. A comparative analysis was carried out and predictive factors related to length of hospital stay were calculated. Results: The reference rate to consultation liaison psychiatry unit was 1.45% of the total older patients hospitalized. Our study demonstrates differences between the groups of older people: the oldest-old group were mainly female (p < 0.001), had more previous psychiatric diagnoses (p < 0.001), physical disabilities (p = 0.02), and neurocognitive disorders (p < 0.001), they used more antipsychotics (p < 0.001), and more frequently had a discharge disposition to a nursing home (p = 0.036). The presence of physical disability (beta = 0.07, p < 0.001) and logtime to referral to consultation liaison psychiatry unit (beta = 0.58, p < 0.001) were associated with increased length of hospital stay. Conclusions: Youngest-old and oldest-old people should be considered as two different types of patients when we consider clinical features. The time to referral to consultation liaison psychiatry unit seems to be a relevant factor associated with length of hospital stay.
The Efficacy of Convalescent Plasma Use in Critically Ill COVID-19 Patients
Authors: Tirnea Livius , Bratosin Felix , Vidican Iulia , Cerbu Bianca , Turaiche Mirela , Timircan Madalina , Margan Madalin-Marius , Marincu Iosif
Published: March 2021

Background and Objectives: On 24 March 2020, the United States Food and Drug Administration (FDA) announced the approval of convalescent plasma therapy for critically ill patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as an emergency investigational new drug. This pilot study from Romania aimed to determine if convalescent plasma transfusion can be beneficial in the treatment of selected critically ill patients diagnosed with a SARS-CoV-2 infection. Materials and Methods: Donor and receiver eligibility for critically ill coronavirus disease 2019 (COVID-19) patients was based on Romanian guidelines issued at the time of the study. Here, we describe the evolution of a total of five eligible patients diagnosed with COVID-19 who received convalescent plasma (CP) in Romania. Results: In spite of our efforts and convalescent plasma administration, three of the five patients did not survive, while the other two recovered completely. Over the course of our five-day laboratory record, the surviving patients had significantly lower values for C-reactive protein, interleukin-6, and white blood cells. Conclusions: This pilot study provides insufficient evidence to determine the efficacy of convalescent plasma use as a therapeutic option for critically ill COVID-19 patients.
Reliability Evaluation of Fan-Out Type 3D Packaging-On-Packaging
Authors: Wang Pao-Hsiung , Huang Yu-Wei , Chiang Kuo-Ning
Published: March 2021

The development of fan-out packaging technology for fine-pitch and high-pin-count applications is a hot topic in semiconductor research. To reduce the package footprint and improve system performance, many applications have adopted packaging-on-packaging (PoP) architecture. Given its inherent characteristics, glass is a good material for high-speed transmission applications. Therefore, this study proposes a fan-out wafer-level packaging (FO-WLP) with glass substrate-type PoP. The reliability life of the proposed FO-WLP was evaluated under thermal cycling conditions through finite element simulations and empirical calculations. Considering the simulation processing time and consistency with the experimentally obtained mean time to failure (MTTF) of the packaging, both two- and three-dimensional finite element models were developed with appropriate mechanical theories, and were verified to have similar MTTFs. Next, the FO-WLP structure was optimized by simulating various design parameters. The coefficient of thermal expansion of the glass substrate exerted the strongest effect on the reliability life under thermal cycling loading. In addition, the upper and lower pad thicknesses and the buffer layer thickness significantly affected the reliability life of both the FO-WLP and the FO-WLP-type PoP.
Looking for Solutions to the Pitfalls of Developing Novel Antibacterials in an Economically Challenging System
Authors: Courtemanche Gilles , Wadanamby Rohini , Kiran Amritanjali , Toro-Alzate Luisa Fernanda , Diggle Mathew , Chakraborty Dipanjan , Blocker Ariel , van Dongen Maarten
Published: March 2021

The increase in antibacterial resistance (ABR) currently equates in the minds of many with the distant fear that certain antibiotics will not work in 30 years on certain bacteria found in places the majority of us never go to. However, in reality, rising ABR already seriously threatens the effectiveness of compounds with which we treat common bacterial infections, which means that ABR is currently and will continue to undermine the foundations of modern medicine, including surgery and cancer treatment in hospitals, cities and countries across the world. That is why ABR is widely considered a global threat and one of the biggest problems of our current civilization. Conversely, antibiotic developments to market are few. Therefore, in this paper, we have illustrated the barriers to antimicrobial R&D the following questions and provided solutions to effective antimicrobial R&D.
Whole Genome Sequence Analysis of Brucella abortus Isolates from Various Regions of South Africa
Authors: Ledwaba Maphuti Betty , Glover Barbara Akorfa , Matle Itumeleng , Profiti Giuseppe , Martelli Pier Luigi , Casadio Rita , Zilli Katiuscia , Janowicz Anna , Marotta Francesca , Garofolo Giuliano , van Heerden Henriette
Published: March 2021

The availability of whole genome sequences in public databases permits genome-wide comparative studies of various bacterial species. Whole genome sequence-single nucleotide polymorphisms (WGS-SNP) analysis has been used in recent studies and allows the discrimination of various Brucella species and strains. In the present study, 13 Brucella spp. strains from cattle of various locations in provinces of South Africa were typed and discriminated. WGS-SNP analysis indicated a maximum pairwise distance ranging from 4 to 77 single nucleotide polymorphisms (SNPs) between the South African Brucella abortus virulent field strains. Moreover, it was shown that the South African B. abortus strains grouped closely to B. abortus strains from Mozambique and Zimbabwe, as well as other Eurasian countries, such as Portugal and India. WGS-SNP analysis of South African B. abortus strains demonstrated that the same genotype circulated in one farm (Farm 1), whereas another farm (Farm 2) in the same province had two different genotypes. This indicated that brucellosis in South Africa spreads within the herd on some farms, whereas the introduction of infected animals is the mode of transmission on other farms. Three B. abortus vaccine S19 strains isolated from tissue and aborted material were identical, even though they originated from different herds and regions of South Africa. This might be due to the incorrect vaccination of animals older than the recommended age of 4–8 months or might be a problem associated with vaccine production.
A Microwave-Assisted Boudouard Reaction: A Highly Effective Reduction of the Greenhouse Gas CO2 to Useful CO Feedstock with Semi-Coke
Authors: Dai Huan , Zhao Hong , Chen Siyuan , Jiang Biao
Published: March 2021

The conversion of CO2 into more synthetically flexible CO is an effective and potential method for CO2 remediation, utilization and carbon emission reduction. In this paper, the reaction of carbon-carbon dioxide (the Boudouard reaction) was performed in a microwave fixed bed reactor using semi-coke (SC) as both the microwave absorber and reactant and was systematically compared with that heated in a conventional thermal field. The effects of the heating source, SC particle size, CO2 flow rate and additives on CO2 conversion and CO output were investigated. By microwave heating (MWH), CO2 conversion reached more than 99% while by conventional heating (CH), the maximum conversion of CO2 was approximately 29% at 900 °C. Meanwhile, for the reaction with 5 wt% barium carbonate added as a promoter, the reaction temperature was significantly reduced to 750 °C with an almost quantitative conversion of CO2. Further kinetic calculations showed that the apparent activation energy of the reaction under microwave heating was 46.3 kJ/mol, which was only one-third of that observed under conventional heating. The microwave-assisted Boudouard reaction with catalytic barium carbonate is a promising method for carbon dioxide utilization.
Zeolites at the Molecular Level: What Can Be Learned from Molecular Modeling
Authors: Broclawik Ewa , Kozyra Paweł , Mitoraj Mariusz , Radoń Mariusz , Rejmak Paweł
Published: March 2021

This review puts the development of molecular modeling methods in the context of their applications to zeolitic active sites. We attempt to highlight the utmost necessity of close cooperation between theory and experiment, resulting both in advances in computational methods and in progress in experimental techniques.
Direct Flavonoid-Focused Chemical Comparison among Three Epimedium Plants by Online Liquid Extraction–High Performance Liquid Chromatography–Tandem Mass Spectrometry
Authors: Xu Xia , Li Ting , Zhang Ke , Cao Yan , Liu Li , Zhang Shilin , Tu Pengfei , Song Yuelin , Zhao Yunfang , Li Jun
Published: March 2021

It is usually a tedious task to profile the chemical composition of a given herbal medicine (HM) using high performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) due to the time-consuming sample preparation and laborious post-acquisition data processing procedures. Even worse, some labile compounds may face degradation risks when exposed to organic solvents for a relatively long period. As one of the most popular HMs, the promising therapeutic benefits of Epimedii Herba (Chinese name: Yinyanghuo) are well defined; however, the chemical profile, and in particular those flavonoids that have been claimed to be responsible for the efficacy, remains largely unknown. Attempts are devoted here to achieve direct LC–MS measurement and efficient post-acquisition data processing, and chemome comparison among three original sources of Epimedii Herba, such as Epimedium sagittatum (Esa), E. pubescens (Epu), and E. koreanum (Eko) was employed to illustrate the strategy utility. A home-made online liquid extraction (OLE) module was introduced at the front of the analytical column to comprehensively transfer the compounds from raw materials onto the LC–MS instrument. A mass defect filtering approach was programmed to efficiently mine the massive LC–MS dataset after which a miniature database was built involving all chemical information of flavonoids from the genus Epimedium to draw a pentagonal frame to rapidly capture potential quasi-molecular ions (mainly [M–H]−). A total of 99 flavonoids (66 in Esa, 84 in Eko, and 66 in Epu) were captured, and structurally annotated by summarizing the mass fragmentation pathways from the mass spectrometric data of authentic compounds and an in-house data library as well. Noteworthily, neutral loss of 144 Da was firstly assigned to the neutral cleavage of rhamnosyl residues. Significant species-differences didn’t occur among their chemical patterns. The current study proposed a robust strategy enabling rapid chemical profiling of, but not limited to, HMs.
Emergence of Macrolide-Resistant Mycoplasma pneumoniae during an Outbreak in a Primary School: Clinical Characterization of Hospitalized Children
Authors: Hubert Daniel , Dumke Roger , Weichert Stefan , Welker Sybille , Tenenbaum Tobias , Schroten Horst
Published: March 2021

Mycoplasma pneumoniae (M. pneumoniae) is a common causative pathogen of community-acquired pneumonia. Here, we report the development of macrolide resistance during a school outbreak of severe M. pneumoniae infections in southwest Germany. We conducted a case series to assess the clinical and laboratory characteristics of hospitalized children with M. pneumonia infection and the prevalence of macrolide-resistant M. pneumoniae (MRMP) in this patient group. We retrospectively analyzed 23 children with serologically (19 patients) and/or PCR (eight patients) confirmed M. pneumoniae infection between October 2019 and December 2019. Most of the 15 hospitalized patients had lower respiratory tract infection (n = 10) and required oxygen therapy (83%). The median length of hospitalization was 7 days (range 3–10 days). In 8/15 patients (53.3%) azithromycin and in 4/15 (26.6%) clarithromycin treatment was applied. However, among the five patients for which extended molecular characterization was performed, sequencing of 23S rRNA revealed no mutation only in the first case, but development of macrolide resistance A2058G in four subsequent cases. Hence, we identified a cluster of hospitalized patients with emerging MRMP. Further studies are warranted to confirm a potential link between macrolide resistance and disease severity.
Hormetic Responses of Photosystem II in Tomato to Botrytis cinerea
Authors: Stamelou Maria-Lavrentia , Sperdouli Ilektra , Pyrri Ioanna , Adamakis Ioannis-Dimosthenis S. , Moustakas Michael
Published: March 2021

Botrytis cinerea, a fungal pathogen that causes gray mold, is damaging more than 200 plant species, and especially tomato. Photosystem II (PSII) responses in tomato (Solanum lycopersicum L.) leaves to Botrytis cinerea spore suspension application were evaluated by chlorophyll fluorescence imaging analysis. Hydrogen peroxide (H2O2) that was detected 30 min after Botrytis application with an increasing trend up to 240 min, is possibly convening tolerance against B. cinerea at short-time exposure, but when increasing at relative longer exposure, is becoming a damaging molecule. In accordance, an enhanced photosystem II (PSII) functionality was observed 30 min after application of B. cinerea, with a higher fraction of absorbed light energy to be directed to photochemistry (ΦPSΙΙ). The concomitant increase in the photoprotective mechanism of non-photochemical quenching of photosynthesis (NPQ) resulted in a significant decrease in the dissipated non-regulated energy (ΦNO), indicating a possible decreased singlet oxygen (1O2) formation, thus specifying a modified reactive oxygen species (ROS) homeostasis. Therefore, 30 min after application of Botrytis spore suspension, before any visual symptoms appeared, defense response mechanisms were triggered, with PSII photochemistry to be adjusted by NPQ in a such way that PSII functionality to be enhanced, but being fully inhibited at the application spot and the adjacent area, after longer exposure (240 min). Hence, the response of tomato PSII to B. cinerea, indicates a hormetic temporal response in terms of “stress defense response” and “toxicity”, expanding the features of hormesis to biotic factors also. The enhanced PSII functionality 30 min after Botrytis application can possible be related with the need of an increased sugar production that is associated with a stronger plant defense potential through the induction of defense genes.
Revenue and Cannibalization: The Effect of Interchangeable Design Confronted Remanufacturing Processing
Authors: Fu Feng , Chen Shuangying , Sun Lin
Published: March 2021

Traditional wisdom suggests that the interchangeable design in process system engineering, such as modularity or commonality design, can lower the manufacturing cost and act as a revenue driver. Moreover, the interchangeable design will be efficient in both assembling for new production and disassembling for remanufacturing. As such, interchangeable design confronted remanufacturing processing often involves a balance of revenue from cost drivers and cannibalization effects from remanufacturing. Therefore, this paper studies how the original equipment manufacturers’ (OEMs’) interchangeable design impacts the remanufacturing decisions, as well as the economy and environment. Specifically, we develop two theoretical models, in which an OEM makes a strategic choice relating to design interchangeability when the remanufacturing operations are undertaken by itself (Model O) or outsourced to third-party remanufacturers (Model T). This study finds that, although the optimal level of interchangeability related to the product design in Model T is lower than that in Model O, the optimal quantity of remanufactured products in the latter scenario is always higher. This suggests that remanufacturing outsourcing deters the OEM’s strategic choice on design interchangeability, which may be consistent with the fact that Lexmark makes its products less interchangeable to avoid remanufacturing from third-party remanufacturers (TPRs). Conversely, although the OEM is always less likely to outsource its remanufacturing operations to independent remanufacturers, remanufacturing outsourcing may be more beneficial for the environment, industry, and society. These key insights on the environmental groups or agencies suggest that remanufacturing outsourcing may be more beneficial for the environment, industry, and society and depends on the OEMs’ attitudes towards its profitability loss. Furthermore, to eliminate the above contrasting effects between the OEMs’ profitability and other issues, two possible remedies, including a revenue-sharing contract and subsidy-incentive mechanism, are provided to achieve a “win-win” situation.
Comparison of Moving-Bed Catalytic Tar Hydrocracking Processes
Authors: Kapustin Vladimir , Chernysheva Elena , Khakimov Roman
Published: March 2021

In recent years, there has been a trend in the global oil industry to improve the proportion of heavy high-sulfur crude oils in the total volume of extracted and processed resources, reserves of which are estimated at over 800 billion metric tons. Therefore, the main line of oil refining is processing of heavy crudes and residua to allow maximum use of the hydrocarbon potential and yield of high-margin products. Hydrogenation processes of heavy raw materials are most attractive in terms of product quality. This article analyzes tar hydrocracking processes that are either in operation or at the stage of full-scale testing. These include Veba Combi-Cracker (VCC), Uniflex, suspended-bed catalyst hydrocracking (ENI), and vacuum residue hydroconversion (TIPS RAS). These technologies use heterogeneous catalysts and are designed to obtain the largest possible amount of liquid products. This article discusses the features of each technology, highlights their advantages and disadvantages, shows the main approaches to process management, and speculates about the development of these technologies. Tar refining is a major process in heavy oil upgrading, and the development of efficient tar-processing methods will influence refinery configurations and management.
Non-Intrusive Load Monitoring Based on Deep Pairwise-Supervised Hashing to Detect Unidentified Appliances
Authors: Zhao Qiang , Xu Yao , Wei Zhenfan , Han Yinghua
Published: March 2021

Non-intrusive load monitoring (NILM) is a fast developing technique for appliances operation recognition in power system monitoring. At present, most NILM algorithms rely on the assumption that all fluctuations in the data stream are triggered by identified appliances. Therefore, NILM of identifying unidentified appliances is still an open challenge. To pursue a scalable solution to energy monitoring for contemporary unidentified appliances, we propose a voltage-current (V-I) trajectory enabled deep pairwise-supervised hashing (DPSH) method for NILM. DPSH performs simultaneous feature learning and hash-code learning with deep neural networks, which shows higher identification accuracy than a benchmark method. DPSH can generate different hash codes to distinguish identified appliances. For unidentified appliances, it generates completely new codes that are different from codes of multiple identified appliances to distinguish them. Experiments on public datasets show that our method can get better F1-score than the benchmark method to achieve state-of-the-art performance in the identification of unidentified appliances, and this method maintains high sustainability to identify other unidentified appliances through retraining. DPSH can be resilient against appliance changes in the house.
Comparison of Canine-Guided Occlusion with Other Occlusal Schemes in Removable Complete Dentures: A Systematic Review
Authors: Ahmed Naseer , Humayun Muhammad , Abbasi Maria , Jamayet Nafij , Habib Syed , Zafar Muhammad
Published: March 2021

Choosing an adequate occlusion is challenging during the construction of artificial dentures, and critical for patient satisfaction. However, there is no conclusive evidence to support which occlusal design is more appropriate. The aim of this systematic review was to evaluate canine-guided occlusion in comparison to other occlusal schemes in removable complete denture wearers. The review was conducted according to the Preferred Reported Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. An extensive search was carried out on (PubMed (National Library of Medicine)), Scopus (Elsevier), Cochrane collaboration (Wiley), Science Direct (Elsevier) and Europe PMC (European Bioinformatics Institute). English and non-English studies were identified using keywords on canine-guided occlusion, denture occlusion, dental occlusion and artificial occlusal schemes. Out of 1759 articles searched, 18 were selected based on the inclusion criteria. The included studies were interpreted for the descriptive analysis for the calculation of occlusal schemes, occlusion assessment parameters, the outcome of the occlusal schemes and types of removable prosthesis used. The systematic analysis of occlusal schemes revealed that canine-guided occlusion is viable compared with other occlusal designs in terms of patient satisfaction, mastication, retention, esthetics, phonetics, oral-health-related quality of life and muscle activity. The type of occlusal scheme influences the complete denture patient’s’ masticatory efficiency, satisfaction, retention, phonetics and esthetics. Nevertheless, physical, physiological, mechanical and psychological factors play a vital role in the success of removable complete dentures. The canine-guided occlusal scheme is preferred because of its simplicity, less time consumption, good masticatory performance, ease of fabrication and modification into bilateral balanced occlusion if required. Further studies are required to reduce the controversies related to jaw relations, gnathology and occlusal schemes in complete dentures.
2020 Quaternary Young Investigator Award: Announcement and Interview with the Winner
Authors:   Quaternary Editorial Office
Published: March 2021

After an extensive voting period, we are proud to present the winner of the Quaternary Young Investigator Award: Dr [...]
Joint Learning of Contour and Structure for Boundary-Preserved Building Extraction
Authors: Liao Cheng , Hu Han , Li Haifeng , Ge Xuming , Chen Min , Li Chuangnong , Zhu Qing
Published: March 2021

Most of the existing approaches to the extraction of buildings from high-resolution orthoimages consider the problem as semantic segmentation, which extracts a pixel-wise mask for buildings and trains end-to-end with manually labeled building maps. However, as buildings are highly structured, such a strategy suffers several problems, such as blurred boundaries and the adhesion to close objects. To alleviate the above problems, we proposed a new strategy that also considers the contours of the buildings. Both the contours and structures of the buildings are jointly learned in the same network. The contours are learnable because the boundary of the mask labels of buildings implicitly represents the contours of buildings. We utilized the building contour information embedded in the labels to optimize the representation of building boundaries, then combined the contour information with multi-scale semantic features to enhance the robustness to image spatial resolution. The experimental results showed that the proposed method achieved 91.64%, 81.34%, and 74.51% intersection over union (IoU) on the WHU, Aerial, and Massachusetts building datasets, and outperformed the state-of-the-art (SOTA) methods. It significantly improved the accuracy of building boundaries, especially for the edges of adjacent buildings. The code is made publicly available.
Semantically Derived Geometric Constraints for MVS Reconstruction of Textureless Areas
Authors: Stathopoulou Elisavet Konstantina , Battisti Roberto , Cernea Dan , Remondino Fabio , Georgopoulos Andreas
Published: March 2021

Conventional multi-view stereo (MVS) approaches based on photo-consistency measures are generally robust, yet often fail in calculating valid depth pixel estimates in low textured areas of the scene. In this study, a novel approach is proposed to tackle this challenge by leveraging semantic priors into a PatchMatch-based MVS in order to increase confidence and support depth and normal map estimation. Semantic class labels on image pixels are used to impose class-specific geometric constraints during multiview stereo, optimising the depth estimation on weakly supported, textureless areas, commonly present in urban scenarios of building facades, indoor scenes, or aerial datasets. Detecting dominant shapes, e.g., planes, with RANSAC, an adjusted cost function is introduced that combines and weighs both photometric and semantic scores propagating, thus, more accurate depth estimates. Being adaptive, it fills in apparent information gaps and smoothing local roughness in problematic regions while at the same time preserves important details. Experiments on benchmark and custom datasets demonstrate the effectiveness of the presented approach.
Detection of a Dust Storm in 2020 by a Multi-Observation Platform over the Northwest China
Authors: Yang Lili , Hu Zhiyuan , Huang Zhongwei , Wang Lina , Han Wenyu , Yang Yanping , Tao Huijie , Wang Jing
Published: March 2021

Dust storms have occurred frequently in northwest China and can dramatically reduce visibility and exacerbate air quality in downwind regions through long-range transport. In order to study the distribution characteristics of dust particles sizes, structures and concentrations in the process of dust storm, especially for the vertical distributions, the multi-observation platform composed of six Lidars and nine aerosol analytical instruments is first used to detect a severe dust storm event, which occurred in Northwest China on 3 May 2020. As a strong weather system process, the dust storm has achieved high intensity and wide range. When the intensity of a dust storm is at its strongest, the ratios of PM2.5 (particulate matter with diameter < 2.5 µm) and PM10 (particulate matter with diameter < 10 µm) (PM2.5/PM10) in cities examined were less than 0.2 and the extinction coefficients became greater than 1 km−1 based on Lidar observations. In addition, the growth rates of PM2.5 were higher than that of PM10. The dust particles mainly concentrated at heights of 2 km, after being transported about 200–300 km, vertical height increased by 1–2 km. Meanwhile, the dust concentration decreased markedly. Furthermore, the depolarization ratio showed that dust in the Tengger Desert was dominated by spherical particles. The linear relationships between 532 nm extinction coefficient and the concentration of PM2.5 and PM10 were found firstly and their R2 were 0.706 to 0.987. Our results could give more information for the physical schemes to simulate dust storms in specific models, which could improve the forecast of dust storms.
Energy-Efficient Navigation of an Autonomous Swarm with Adaptive Consciousness
Authors: Yasin Jawad Naveed , Mahboob Huma , Haghbayan Mohammad-Hashem , Yasin Muhammad Mehboob , Plosila Juha
Published: March 2021

The focus of this work is to analyze the behavior of an autonomous swarm, in which only the leader or a dedicated set of agents can take intelligent decisions with other agents just reacting to the information that is received by those dedicated agents, when the swarm comes across stationary or dynamic obstacles. An energy-aware information management algorithm is proposed to avoid over-sensation in order to optimize the sensing energy based on the amount of information obtained from the environment. The information that is needed from each agent is determined by the swarm’s self-awareness in the space domain, i.e., its self-localization characteristics. A swarm of drones as a multi-agent system is considered to be a distributed wireless sensor network that is able to share information inside the swarm and make decisions accordingly. The proposed algorithm reduces the power that is consumed by individual agents due to the use of ranging sensors for observing the environment for safe navigation. This is because only the leader or a dedicated set of agents will turn on their sensors and observe the environment, whereas other agents in the swarm will only be listening to their leader’s translated coordinates and the whereabouts of any detected obstacles w.r.t. the leader. Instead of systematically turning on the sensors to avoid potential collisions with moving obstacles, the follower agents themselves decide on when to turn on their sensors, resulting in further reduction of overall power consumption of the whole swarm. The simulation results show that the swarm maintains the desired formation and efficiently avoids collisions with encountered obstacles, based on the cross-referencing feedback between the swarm agents.
CONIC: Contour Optimized Non-Iterative Clustering Superpixel Segmentation
Authors: Li Cheng , Guo Baolong , Liao Nannan , Gong Jianglei , Han Xiaodong , Hou Shuwei , Chen Zhijie , He Wangpeng
Published: March 2021

Superpixels group perceptually similar pixels into homogeneous sub-regions that act as meaningful features for advanced tasks. However, there is still a contradiction between color homogeneity and shape regularity in existing algorithms, which hinders their performance in further processing. In this work, a novel Contour Optimized Non-Iterative Clustering (CONIC) method is presented. It incorporates contour prior into the non-iterative clustering framework, aiming to provide a balanced trade-off between segmentation accuracy and visual uniformity. After the conventional grid sampling initialization, a regional inter-seed correlation is first established by the joint color-spatial-contour distance. It then guides a global redistribution of all seeds to modify the number and positions iteratively. This is done to avoid clustering falling into the local optimum and achieve the exact number of user-expectation. During the clustering process, an improved feature distance is elaborated to measure the color similarity that considers contour constraint and prevents the boundary pixels from being wrongly assigned. Consequently, superpixels acquire better visual quality and their boundaries are more consistent with the object contours. Experimental results show that CONIC performs as well as or even better than the state-of-the-art superpixel segmentation algorithms, in terms of both efficiency and segmentation effects.
Using TPI to Map Spatial and Temporal Variations of Significant Coastal Upwelling in the Northern South China Sea
Authors: Shi Weian , Huang Zhi , Hu Jianyu
Published: March 2021

Based on Himawari-8 Sea Surface Temperature (SST) data and the semi-automatic Topographic Position Index (TPI)-based mapping method, this study maps the significant coastal upwelling in the northern South China Sea (NSCS). The results show that the Minnan coastal upwelling mainly occurs within 100 km off the south coast of Fujian; the Yuedong coastal upwelling appears to the east of Pearl River Estuary, limited to the area shallower than 40 m; and the Qiongdong coastal upwelling occurs most frequently in the area shallower than 75 m off the east coast of Hainan Island. Based on the results, this paper quantitatively describes the temporal and spatial variations of upwelling duration, influence area, upwelling SST anomaly, and chlorophyll-a (Chl-a) increase. Different coastal upwelling regions in the NSCS are significantly different in characteristics. The Qiongdong coastal upwelling has the longest duration and occurs most frequently, the Yuedong coastal upwelling has the largest influence area and Chl-a increase, and the Minnan coastal upwelling is quite strong in the NSCS.