bluuebunny/crossref_metadata_2025 huggingface dataset:
Greenwash and green brand equity: The mediating role of green brand image, green satisfaction, and green trust, and the moderating role of green concern
Authors: Ha Minh-Tri
Published: November 2022

This study explores whether and, if so, how efficiently consumers’ greenwashing (GW) influences green brand equity (GBE) by integrating the mediation role of green brand image (GBI), green satisfaction (GSA), and green trust (GTR) and the moderating role of green concern (GC), using the legitimacy and signaling theories. A quantitative study was conducted by means of a questionnaire-based survey using a cluster random sampling technique with a sample of 436 consumers who purchased electronic products in supermarkets in Vietnam. A partial least squares structural equation modelling (PLS-SEM) approach was used to analyze data. Our findings show that GW is not significantly associated with GBE, possibly due to the halo effect. The findings also show that GW is adversely related to GBI, GSA, and GTR, which would positively influence GBE, except for the GW-GSA relationship. This means that, although GW does not directly affect GBE, it does indirectly influence it via GBI and GTR. Furthermore, GC significantly moderates the relationship between GW and GBE. Our work is the first to combine GBI, GSA, and GTR as mediators, and GC as a moderator using PLS-SEM approach to advance the theory of green brand equity on green marketing and to contribute significantly toward a unified theory of brand equity. Furthermore, our findings extend our understanding regarding the different mechanisms for which GBI and GTR play as mediators, and with GC as a moderator in the GW-GBE relationship in the electronics products setting of Vietnamese consumers.
Universal admission screening for COVID-19 using quantitative antigen testing and questionnaire screening to prevent nosocomial spread
Authors: Morishima Masayo , Kimura Muneyoshi , Sakoh Takashi , Yamamuro Ryosuke , Ogura Sho , Takahashi Namiko , Baba Masaru , Herai Takashi , Endo Shigeyuki , Taniguchi Shuichi , Araoka Hideki
Published: November 2022

Background

In this study, we investigated diagnostic accuracy of quantitative severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen testing and whether universal screening was effective to prevent a nosocomial outbreak of coronavirus disease 2019 (COVID-19).

Methods

All adult patients admitted to an acute-care hospital in Tokyo, Japan, after receiving LUMIPULSE SARS-CoV-2 Ag using a nasopharyngeal swab and a brief questionnaire to evaluate symptoms and exposures from December 3, 2020 to March 20, 2021 were included.

Results

Of the 5191 patients, 53 were antigen-positive, 19 were inconclusive and 5119 were negative. The sensitivity and specificity (positive or inconclusive results) of the quantitative antigen test for COVID-19 diagnosis at admission was 0.957 (95% confidence interval [CI]: 0.855–0.995) and 0.995 (95% CI: 0.992–0.997), respectively. Six asymptomatic patients were identified on admission. Two patients were antigen-negative and diagnosed with COVID-19 later; however, they had been isolated prior to diagnosis because both had symptoms of COVID-19 and exposure. No nosocomial infections occurred during the period.

Conclusion

Quantitative SARS-CoV-2 antigen testing was found to be valid for the early detection of asymptomatic COVID-19 patients as a universal screening test on admission.
Ocular-following responses in school-age children
Authors: Miladinović Aleksandar , Quaia Christian , Ajčević Miloš , Diplotti Laura , Cumming Bruce G. , Pensiero Stefano , Accardo Agostino
Published: November 2022

Ocular following eye movements have provided insights into how the visual system of humans and monkeys processes motion. Recently, it has been shown that they also reliably reveal stereoanomalies, and, thus, might have clinical applications. Their translation from research to clinical setting has however been hindered by their small size, which makes them difficult to record, and by a lack of data about their properties in sizable populations. Notably, they have so far only been recorded in adults. We recorded ocular following responses (OFRs)–defined as the change in eye position in the 80–160 ms time window following the motion onset of a large textured stimulus–in 14 school-age children (6 to 13 years old, 9 males and 5 females), under recording conditions that closely mimic a clinical setting. The OFRs were acquired non-invasively by a custom developed high-resolution video-oculography system, described in this study. With the developed system we were able to non-invasively detect OFRs in all children in short recording sessions. Across subjects, we observed a large variability in the magnitude of the movements (by a factor of 4); OFR magnitude was however not correlated with age. A power analysis indicates that even considerably smaller movements could be detected. We conclude that the ocular following system is well developed by age six, and OFRs can be recorded non-invasively in young children in a clinical setting.
Validation of quantitative real-time PCR reference genes and spatial expression profiles of detoxication-related genes under pesticide induction in honey bee, Apis mellifera
Authors: Kim YeongHo , Kim Hyemin , Cha JooHeon , Lee Si Hyeock , Kim Young Ho
Published: November 2022

Recently, pesticides have been suggested to be one of the factors responsible for the large-scale decline in honey bee populations, including colony collapse disorder. The identification of the genes that respond to pesticide exposure based on their expression is essential for understanding the xenobiotic detoxification metabolism in honey bees. For the accurate determination of target gene expression by quantitative real-time PCR, the expression stability of reference genes should be validated in honey bees exposed to various pesticides. Therefore, in this study, to select the optimal reference genes, we analyzed the amplification efficiencies of five candidate reference genes (RPS5, RPS18, GAPDH, ARF1, and RAD1a) and their expression stability values using four programs (geNorm, NormFinder, BestKeeper, and RefFinder) across samples of five body parts (head, thorax, gut, fat body, and carcass) from honey bees exposed to seven pesticides (acetamiprid, imidacloprid, flupyradifurone, fenitrothion, carbaryl, amitraz, and bifenthrin). Among these five candidate genes, a combination of RAD1a and RPS18 was suggested for target gene normalization. Subsequently, expression levels of six genes (AChE1, CYP9Q1, CYP9Q2, CYP9Q3, CAT, and SOD1) were normalized with a combination of RAD1a and RPS18 in the different body parts from honey bees exposed to pesticides. Among the six genes in the five body parts, the expression of SOD1 in the head, fat body, and carcass was significantly induced by six pesticides. In addition, among seven pesticides, flupyradifurone statistically induced expression levels of five genes in the fat body.
The emergent loop of interdisciplinary exchange
Authors: O’Dempsey Kellie
Published: November 2022

This exegetical analysis of performance drawing highlights its character as an interdisciplinary process. Specifically, I demonstrate that the interrelation of drawing and performance, that occurs in the active, live-audience drawing processes, is a unique interdisciplinary exchange. This analysis utilizes the concept of ‘intra-action’, as defined by Karen Barad, to reveal the multidirectional and emergent processes of the interdisciplinary exchange exhibited through drawing as performance. Furthermore, this interdisciplinary exchange is likened to a Möbius loop. Exploring ‘intra-action’ and the Möbius loop analogy, I examine the interdisciplinary drawing process through the cooperative performance drawing work initiated by myself, Kellie O’Dempsey (Australia) and Jennifer Wroblewski (United States): Resistance Movement (2017–18). This work exemplifies the interdisciplinary process of performance drawing as the initiator for creative exchange. To examine this proposition, I isolate the components of drawing, performance and the spatio-temporal, and analyse how these components operate through Resistance Movement. While Barad’s theory of intra-action relates to the entanglement of matter in the universe, I use the theory to explore the entangled aspects of performance drawing that create a single, unified experience for both artists and viewers. The three elements – drawing, performance and the spatio-temporal – combine, resulting in intra-action. Thus, creating a unique, interdisciplinary event.
Demise of Legal Aid? Access to Justice and Social Welfare Law after Austerity
Authors: Newman Daniel , Robins Jon
Published: November 2022

Access to justice in England and Wales has been undermined by the Legal Aid, Sentencing and Punishment of Offenders Act 2012. These cuts to legal aid came as part of the Conservative-Liberal Democrat Coalition government’s austerity programme and they represent part of a deeper legacy of antipathy towards state funding of legal services over recent decades. This socio-legal paper draws on interviews across four case studies with those on the frontline of the legal aid sector to draw out the implications of the LASPO cuts, and the wider disdain of successive governments for legal aid, for social welfare law. Vulnerability theory is used to highlight the importance of the legal aid scheme and the threat posed by the cuts. The paper makes an argument that access to justice is a cause that needs to be championed for the good of all in society.

Keywords: access to justice; legal aid; social welfare law; austerity; vulnerability.
Reflections on the Judicial Case Management Experiments of Sir Francis Newbolt
Authors: Reynolds Michael
Published: November 2022

In two earlier articles published in Amicus Curiae, a pioneering form of case management was reviewed. Essentially these essays revealed that Sir Francis Newbolt, an Official Referee, was the pioneer in this processual innovation, in his work between 1920 and 1936. His procedural experiments and advances laid the foundation for a distinctive process adopted by the Official Referees’ Court which survives to this day, albeit enhanced and adapted to meet the challenges of the digital age. In many respects, and as suggested in the earlier contributions, Newbolt was far ahead of his times, although it is important to appreciate also that he was driven largely by the impact of post-World War I austerity and the economic pressures of the Great Depression which stretched judicial resources. In some respects, there may be an almost historical correlation between his times and today—a period of austerity followed by an unexpected pandemic, exacerbated by interruptions to trading relationships. The pandemic of 1918 is said to have had greater consequences than the World War, imperial preference, protectionism and the depression. The experience of those times may have some relevance to our own. In this article, however, a comparison is drawn between Newbolt’s ‘Scheme’ and the subsequent access to justice reforms in England and Wales, demonstrating in many respects a certain degree of equivalence in the objectives of Lord Woolf and Sir Francis. This may be equated with my experience as a solicitor who practised in the Official Referees Court, which then became the Technology and Construction Court. That court inherited the practice derived from Newbolt’s experiments and enabled a more efficient form of case management broadly conforming to the objectives of access to justice.

Keywords: case management; official referees; innovative procedure.
Approved handing down of judgment: England & Wales - Bates v The Post Office Ltd (No 6: Horizon Issues) Rev 1 16 December 2019
Authors: Case Judgment: England & Wales
Published: November 2022

Case citation:

Bates v The Post Office Ltd (No 6: Horizon Issues) Rev 1 16 December 2019 Approved handing down of Judgment by Fraser J, No. QB-2016-004710, Supplement, 18 Digital Evidence and Electronic Signature Law Review (2021)
Case Transcript - Bates v Post Office Limited, TLQ 17 4055 Day 4 14 March 2019
Authors: Case Transcript England & Wales
Published: November 2022

Case Transcript - Bates v Post Office Limited, TLQ 17 4055

Day 4 14 March 2019

England & Wales; theft; electronic evidence; Post Office Horizon System; ‘reliability’ of computers
Case Transcript - Bates v Post Office Limited, TLQ 17 4055 Day 7 20 March 2019
Authors: Case Transcript England & Wales
Published: November 2022

Case Transcript - Bates v Post Office Limited, TLQ 17 4055

Day 7 20 March 2019

England & Wales; theft; electronic evidence; Post Office Horizon System; ‘reliability’ of computers
Case Transcript - Bates v Post Office Limited, TLQ 17 4055 Day 15 5 June 2019
Authors: Case Transcript England & Wales
Published: November 2022

Case Transcript - Bates v Post Office Limited, TLQ 17 4055

Day 15 5 June 2019

England & Wales; theft; electronic evidence; Post Office Horizon System; ‘reliability’ of computers
Case Transcript - Bates v Post Office Limited, TLQ 17 4055 Day 20 14 June 2019
Authors: Case Transcript England & Wales
Published: November 2022

Case Transcript - Bates v Post Office Limited, TLQ 17 4055

Day 20 14 June 2019

England & Wales; theft; electronic evidence; Post Office Horizon System; ‘reliability’ of computers
Case Transcript - Bates v Post Office Limited, TLQ 17 4055 Day 21 1 July 2019
Authors: Case Transcript England & Wales
Published: November 2022

Case Transcript - Bates v Post Office Limited, TLQ 17 4055

Day 21 1 July 2019

England & Wales; theft; electronic evidence; Post Office Horizon System; ‘reliability’ of computers
Scandal at the Post Office
Authors: Marshall Paul
Published: November 2022

The Post Office Horizon scandal in the United Kingdom, possibly the most extensive miscarriage of justice in English legal history, was caused by a number of interrelated factors. These include: the legal presumption that computers are reliable; the unwillingness and failure of judges to order appropriate and necessary disclosure of documents by the Post Office, where required to ensure fairness at trial; the unethical conduct by the board and management of the Post Office; failure by government – the Post Office’s owner, and questionable litigation strategies adopted by the Post Office’s lawyers.

Index words: Post Office; Horizon IT system; disclosure; discovery; electronic evidence; ethics; politics
Simplification to the Immigration Rules and its effects
Authors: Gurung Shreevana
Published: November 2022

The Immigration Rules hold paramount importance in controlling and monitoring the UK borders and non-British population. These rules expansively dictate the boundaries and movements of every non-British citizen; hence, they are relied upon widely by public bodies and the judiciary. The Immigration Act 1971 was initially passed to control the UK immigration system. However, the law under this statute has been developed on an ad hoc basis, which has resulted in a convoluted set of laws being established.

The complexity of these laws stems predominantly from statutory provisions being continuously updated or amended and subjective judicial interpretations of the Immigration Rules being mostly inconsistent. These issues are exacerbated further by the ever-evolving social change surrounding immigration. These difficulties concerning the current Immigration Rules have led the Law Commission in its 13th programme of law reform to propose the idea that Immigration Rules need simplification urgently.
Percepción subjetiva del esfuerzo, la ansiedad y la autoconfianza en jugadores de fútbol semiprofesionales
Authors:   Departamento de Educación Física y Deportiva, Universidad de Granada, España, Castillo-Rodríguez Alfonso , Ruiz-Sánchez Esteban ,   Departamento de Educación Física y Deportiva, Universidad de Granada, España, Martins Fernando ,   Instituto Politécnico de Coimbra, ESEC, IIA, NIEFI, UNICID, ROBOCORP, Coimbra, Portugal,   Instituto de Telecomunicações, Covilhã, Portugal, Onetti-Onetti Wanesa ,   Facultad de Educación, Universidad Internacional de la Rioja, España
Published: November 2022

Objetivo: analizar la percepción subjetiva del esfuerzo y de los estados de ansiedad (cognitiva y somática), autoconfianza en jugadores de fútbol semiprofesionales. Método: han participado 18 jugadores españoles semiprofesionales con experiencia mínima de tres años en la categoría nacional (edad: 26.4 años, peso: 75.8 kg, altura: 181 cm) clasificados en dos porteros, tres defensas centrales, tres defensas laterales, cuatro centrocampistas, tres extremos y tres delanteros. Se analizaron 10 partidos (cinco como locales y cinco como visitantes) y se usó el cuestionario Competitive State Anxiety Inventory 2 y escala Category Ratio 10 de Borg para la percepción subjetiva del esfuerzo. Se realizó el test de análisis de la varianza de un factor (posición de juego) y t-test para muestras independientes (localización y rol). Resultados: se han obtenido diferencias significativas en estas variables psicofisiológicas en relación con las posiciones de juego, localización y rol de juego. Conclusión: en este estudio de caso, los jugadores con rol defensivo y los que juegan como visitantes tienen menor percepción subjetiva del esfuerzo y los atacantes presentan mayor ansiedad somática y autoconfianza previos a la competición deportiva.
organização do trabalho escolar e os especialistas da educação
Authors: Pizzi Laura Cristina Vieira
Published: November 2022

Resumo: A organização do trabalho escolar ainda é vista por alguns sob o paradigma da organização do trabalho fabril capitalista. Tal abordagem permeou muitos estudos, sobretudo nos anos 80. As analogias envolvendo escola capitalista e empresa capitalista fermentaram a busca de elementos empíricos e teóricos que mostrassem as evidências destes vínculos. Este estudo, nos seus limites, tem como eixo estas mesmas preocupações.
Um dos personagens envolvidos nesta discussão é o chamado “especialista da educação”. Os especialistas são aqueles profissionais formados no curso de Pedagogia nas diferentes Habilitações oferecidas, entre as quais se destacam a Orientação Educacional, a Supervisão Escolar, a Administração Escola e a Inspeção Escolar. O trabalho destes profissionais se dá, basicamente, fora da sala de aula, em atividades de coordenação e assessoria pedagógica a alunos e professores. Apesar de serem profissionais que há muito atuavam nas redes de ensino, foi no período da ditadura militar que os cursos de pedagogia passaram a formá-los generalizadamente, fruto do modelo político-econômico dependente e da pedagogia tecnicista importada dos Estados Unidos, via acordos MEC-USAID.
Este trabalho, síntese da pesquisa com o mesmo nome desenvolvida durante o mestrado, teve como objetivo avaliar a participação dos especialistas da educação na organização do trabalho das escolas públicas, tendo por base as principais críticas ao seu papel. Entre as mais contundentes, estão as que identificam os especialistas como “agentes ideológicos do capital” no meio escolar, o que significa afirmar o seu papel de controladores de alunos e professores. Os alunos ficavam a cargo dos Orientadores Educacionais, que cuidavam dos problemas disciplinares e do encaminhamento profissional. Os Supervisores Escolares eram responsáveis pelo aspecto didático-pedagógico do trabalho desenvolvido pelos professores. Entre os especialistas formados nas habilitações, estes eram os mais comuns nas escolas, pois os Inspetores e Administradores eram cargos de confiança dos políticos no poder, como é ainda hoje em muitos Municípios e Estados do país. Por esse motivo, os Orientadores e Supervisores foram privilegiados neste estudo, juntamente com os professores.
Palavras-chave: trabalho escolar; capitalismo; especialistas da educação.
"romantismo", o "enciclopedismo" e o "criticismo"
Authors: Da Silva Sérgio Pereira
Published: November 2022

Este artigo é fruto de uma pesquisa sobre o magistério da Filosofia, no Ensino Médio, em Uberlândia. Revela metodologias espontaneístas e conteudistas, no bojo das concepções aqui denominadas de "romântica", "enciclopédica" e "crítica''. 
Palavras-chave: Educação no ensino médio; Filosofia; Uberlândia.
Abstract: This article is product of a research about the teaching of Philosophy, at high school, in Uberlândia. lt tries to show some methodologies, associated to conceptions called here "romantic", "encyclopaedic" and "critic".
Keywords: teaching at high school; Philosophy; Uberlândia.
que é um "bom professor"?
Authors: Ghiraldelli Jr. Paulo
Published: November 2022

Resumo: O texto trata da evolução da ideia de "bom professor" no contexto da modernidade e da época contemporânea. Considera, então, três fases histórico-filosóficas: o professor segundo o discurso pedagógico humanista; o professor segundo o discurso pedagógico da era "sociedade do trabalho" e o professor segundo o discurso pedagógico "tecnicista".
Palavras-chave: professor; filosofia; história; infância; pedagogia.
 
Abstract: This paper deals with the idea of "good teacher" in the context of modernity and contemporary age. Three historico-philosophical phases are taken here under consideration: the teacher in the pedagogical discourse of humanism; the teacher in the pedagogical discourse of the "laboring society" and, finally, the teacher accordingly to the pedagogical discourse of "technicism".
VITA, alvaro. Justiça liberal, argumentos liberais contra o neoliberalismo. São Paulo, Ed. Paz e Terra, 1993, 131 pp.
Authors: Bonela Alcino Eduardo
Published: November 2022

O livro, com algumas modificações, é a dissertação de mestrado, defendida na Universidade de São Paulo em 1992, por Alvaro de Vita, bacharel em Ciências Sociais e mestre em Ciência Politica. É um trabalho sobre teoria liberal normativa, especialmente sobre a teoria da justiça de John Rawls, pensador contemporâneo que retoma o contratualismo e a ética de Kant para elaborar uma filosofia moral e politica que se apresente como alternativa viável à tradição utilitarista. Além da teoria de Rawls, Vita também discute pontos da teoria de Ronald Dworkin, referencial da abordagem chamada "baseada em direitos". O estudo levado a cabo por Vita investiga a moralidade liberal, que prioriza direitos face às considerações utilitárias.
Tal moralidade é sintetizada por ele em duas teses: 1) O Estado deve proteger um conjunto de direitos básicos dos cidadãos; 2) O Estado deve ser neutro quanto às diversas formas de vida que os cidadãos razoavelmente escolham para si em função de suas "concepções de bem", ou seja, o Estado não deve impor uma concepção de bem humano. Na dissertação aqui resenhada, Vita se concentrará na tese 1.
Cults, Practices and Beliefs: the Symbolism of Geodiversity in Christianity
Authors: Ferreira Arlon Cândido , Vallejo Luiz Renato , Rocha Leonardo Cristian , Azevedo Úrsula Ruchkys , Travassos Luiz Eduardo Panisset , Teixeira Rodrigo Corrêa
Published: November 2022

From the expansion of monotheism, as sacred laws, symbolisms, beliefs and practices began to be produced, thus emerging the sacred books. The biblical text influenced - and still - influences the direction of Western civilization. This work aims to raise and discuss aspects related to Geodiversity, here understood as the allusion of the abiotic characteristics of nature, present in the Holly Bible, as well as its use in cults, practices and beliefs measured by symbolic elements. To carry out this survey, the texts of the King James Version Bible (KJV) were used. After the survey of the elements of Geodiversity, they were divided by typologies to facilitate interpretation, tables were made that include the name of the elements, and their geological / geographical description, their use in the Bible, when applicable. When analyzing the abiotic elements, it was perceived, initially that the elements of Geodiversity, are used as nouns, referring properly to the element, but in several situations, they are used symbolically. Science and religion are generally perceived through dichotomization, but the set of religious manifestations that are open to external analysis is still of scientific interest. In this article, we verify the importance of Geodiversity in the study of the Bible and, consequently, the analysis of the elements of Geodiversity present in the sacred books and their symbology, leads to the reflection that this approach can help in a better understanding and interpretation of biblical facts considering another perspective, giving more consistency and authenticity, whether it deals with the abiotic scenario present in the divine revelations, it is, therefore, a Geoteology.
Evaluation of mustard genotypes [Brassica juncea (L.) Czern and Coss] for quantitative traits and character association of seed yield and yield components at sub Himalayan region of West Bengal (India)
Authors: Sur Bijaya , Rout Sanghamitra , Singla Saurav , Mandal Rupsanatan , Nath Sahanob , Maying Bilin , Sadhu Supratim , Chakraborty Moumita , Hijam Lakshmi , Debnath Manoj Kanti , Roy Suvendu Kumar
Published: November 2022

Brassica juncea is an important industrial and commercial oilseed crop grown primarily in India. This study aimed to assess 56 genotypes of Indian mustard to quantify genetic diversity, which aids the breeder in identifying genetically divergent parents to evaluate the proportional contributions of various components towards overall divergence. All the 56 Indian mustard genotypes were tested in RBD with three replications for 2 consecutive years i.e. 2016-17 and 2017-18 during the rabi season. Observations were recorded for 11 yield and its attributing traits. The findings revealed that height up to first branching, aphid count, penetration force and seed yield per plant had maximum PCV and GCV signifying that genetic factors have a greater impact on the inflow of these traits. Height up to first branching, secondary branches per plant, primary branches per plant, siliquae per plant, aphid count and 1000 seed weight had strong heritability combined with GA as % of mean. These indicate that the traits were controlled by additive gene action. Seed yield per plant was significantly correlated with penetration force and siliquae per plant. As a result, it's reasonable to predict that improving these traits by selection, could lead to significant yield gains. Four of the eleven PCs had eigen values greater than 1.0, accounting for 69.94% of the variance. PC I, which explained 30.31% of the overall variance. Mahalanobis D2 statistics revealed considerable genetic diversity among the genotypes. 56 genotypes were distributed into 7 clusters. This is anticipated that genotypes within a cluster are almost genetically related to one another. Cluster VII and II showed maximum inter-cluster divergence. From a breeding perspective, a divergence analysis revealed that genotypes like SKJM-05, RNWR-09-3, RW-351, B-85, DRMR-4001, RGN-386, TM52 276 and SKM-1313 can be selected as genetically divergent parents for hybridization to obtain desirable segregants.
Lectotypification of names in the genus Eragrostis Wolf (Poaceae)
Authors: Jaiswal Shubham , Tiwari Smita , Prasad Dileshwar , Yadav Rekha , K Madhukar Virendra , Agnihotri Priyanka
Published: November 2022

In the present work 3 names within the genus Eragrostis viz, Eragrostis coarctata, E. plana and E. superba have been typified. Lectotypification has been performed for E. coarctata and Second-step lectotypification has been done for the names E. plana and E. superba. For lectotypification, rules and recommendations proposed by the International Code of Nomenclature for algae, fungi and plants (ICN), have been strictly followed. The selection of lectotypes are explained and the images of selected lectotypes are provided.
Quantitative detection of anti-PLA2R antibodies targeting different epitopes and its clinical application in primary membranous nephropathy
Authors: Qin Yuan , Wu Qingqing , Sheng Huiming , Li Ting , Liu Xiaobin , Yang Xue , Lin Bo , Zhou Xiumei , Jin Juan , Wang Liang , Huang Biao , He Qiang , Hu Zhigang
Published: November 2022

Objectives

This study aimed to establish time-resolved fluorescence immunoassays to quantitatively detect the autoantibodies targeting different epitopes of M-type phospholipase A2 receptor (PLA2R) and evaluate its clinical application in primary membranous nephropathy (PMN).

Methods

PLA2R and its reactive epitope-specific IgG/IgG4 time-resolved fluorescence immunoassays (TRFIAs) were established using europium-labeled anti-human IgG/IgG4 antibodies, recombinant proteins, and patient serum. The levels of IgG/IgG4 targeting PLA2R and its epitopes in PMN patient serum were detected, and the relationship between epitope spreading of PLA2R and the severity of patients with PMN was evaluated.

Results

The TRFIAs established in this study could quantitatively detect PLA2R and its epitope-specific IgG and IgG4. Sera from 59 patients with PMN were subjected to detection using anti-PLA2R IgG and anti-PLA2R IgG4. Among them, 46 and 54 patients were found positive for PLA2R antibodies, respectively. Moreover, the levels of PLA2R antibodies were strongly correlated with the severity of patients with PMN. Patients who were detected to have two or more epitopes had more serious renal injury.

Conclusions

PLA2R domain-specific IgG/IgG4 TRFIAs were established in this study, and detection with anti-PLA2R IgG4 could more sensitively screen the reactivity of patients to the PLA2R domain. Moreover, detection epitope spreading of PLA2R was confirmed which is related to the severity of patients with PMN.
Az etika digitális lemészárlása. Avagy az erkölcs és a profit kellemetlen hálótársak, valahogy soha nem elég nekik egy takaró
Authors: Novák Pál
Published: November 2022

Nehéz lenne megmondani, hogy a hétköznapjainkat a „gyorsaság”, „a nem elég” és a „pörgés” irányába taszigáló fogyasztói kultúránkban vajon mennyire összeegyeztethető Francis Crick és Immanuel Kant egymásnak elemeiben ellentmondó megállapítása. Míg Kant világpolgári felfogásában „a filozófia nem pusztán tudomány, hanem minden ismeretnek és tudománynak az emberi ész lényegi céljaira való vonatkoztatása, az emberi ész törvényhozója”, addig Francis Crick, a Nobel díjas biológus szavai, melyek erősen kapcsolódnak a neuro-marketinghez így szólnak: „Te, az örömeid és bánataid, emlékeid és ambícióid, személyes identitásod és szabad akaratod valójában nem több, mint idegsejtek hatalmas csoportjának viselkedése”. Vajon eligazítást adna-e a bőségben megfáradt, termékként ideológiákat is vásárló fogyasztóknak, vagy szolgáltatóknak a régi könyvtárak öreg polcain porosodó etikai, filozófiai tételek alaposabb ismerete? Álláspontom szerint igen. Az előadás és a vitairat a 21. század társadalomtudományokat megtépázó technológiai-profitorientált hozzáállásával szemben fogja vizsgálni az alapvető értékek fontosságát és az okot, amiért nem látjuk már annak őket.
Analysis of the Application of Green Building Concepts in Educational Buildings Case Study: Building B, Faculty of Biology UGM Yogyakarta
Authors: Ayuningtyas Nurina Vidya , Adianti Istiana
Published: November 2022

Abstract. The building sector is one of the world's largest energy consumers. This problem is further exacerbated by our dependence on non-renewable energy sources. More than 90% of the world's energy comes from fossil energy including oil, natural gas and coal whose availability in nature continues to decrease because they cannot be renewed. Green design is a reaction to the global environmental crisis. Green design which also leads to eco design, or design for environment is the art of designing physical objects and the surrounding environment to balance sustainable principles with economic, social, and ecological aspects. As a construction actor, you are required to be able to produce a building design that does not require too much energy consumption and also of course considers the comfort of the occupants in it. In an effort to develop the campus, Universitas Gadjah Mada seeks to make the UGM campus a Green Campus. One of the buildings at UGM and the first building at UGM to be certified as a green building by the Green Building Council Indonesia (GBCI), namely Building B, Faculty of Biology UGM with 43% energy savings points, 30% water and 65% efforts to utilize local materials . From the Energy sector, this building applies a small percentage of window/glass openings, uses reflective paint so as to minimize heat that will be transmitted into the building, external shading devices (shading), applies insulation on the roof, a variable refrigerant type HVAC system (VRF). ) with a high COP so that it can save more energy and use artificial lighting / energy-saving lamps. From the water sector, using a toilet fixture with a low flow so that it can save water. Meanwhile, the use of materials includes the use of clay tiles, hebel walls and other materials that are local products.
Covid-19 Responsive Public Space Signage Design for Children from Physical Distancing Aspects at Lapangan Merdeka, Pematangsiantar
Authors: Pasaribu Nanda Rosaline , Ristianti Novia Sari
Published: November 2022

Abstract. Adaptation of public space users requires COVID 19 responsive design elements to support public health, especially for children aged 7-12 years who are classified as vulnerable and still need public space to play and socialize. The Lapangan Merdeka area is a public open space where children make up about 60% of visitors. Currently, there is still negligence in the application of health protocols. This study considers design requirements in implementing physical distancing, such as signage aspects. The quantitative method is used in this research. The analysis stages consisted of identifying the child's characteristics through behavior-setting and place-setting; and formulating design element needs through signage aspects such as directional signage, informative signage, and identification signage. The research result shows three-element of signage. First is directional signage, such as a circulation regulation on the surfaces of the jogging track. It is used for the signing facility on the jogging track. It uses a board stuck in the ground and is placed in the area leading to the field, and uses an information board with 1-1.5 meters on the jogging track. Second, informative signage such as an x-banner at all facility points. Third, identification signage such as a board containing an area plan and placed near the words "Lapangan Merdeka."
Analysis of the Advantages and Disadvantages of Using Autodesk Revit for the Dean Building of the Faculty of Education, Universitas Negeri Semarang
Authors: Yuvita Rizky Lucky , Budiwirawan Agung
Published: November 2022

Abstract. Building Information Modeling (BIM) as a supporting software of the fourth phase of the industrial revolution in Indonesia in the construction sector is a digital technology mode to increase the efficiency and accuracy of construction work to avoid mistakes during the planning to the implementation period. This study uses the Autodesk Revit application to provide the output of working drawings, 3D modeling, and calculate the volume and cost of work carried out in the Dean building of the Faculty of Education, Universitas Negeri Semarang. The purpose of this study is to determine the advantages and disadvantages of using Autodesk Revit for planning the Dean building of the Faculty of Education, Universitas Negeri Semarang. The results of this study are the advantages of using Autodesk Revit for building modeling, including providing convenience with design integration in one application, and avoiding crashes between designs. Autodesk Revit also has weaknesses, such as the need for high hardware specifications on the device, high license prices, the application it is necessary to create a new material family according to the design and size required, Autodesk Revit cannot calculate the preparatory work because the calculation of the volume and cost of Autodesk Revit work is limited to data input.
Paragangliomas of the head and neck: a contemporary review
Authors: Graham Nathan J , Smith Joshua D , Else Tobias , Basura Gregory J
Published: November 2022

Head and neck paragangliomas (HNPGLs) are slow-growing, vascular, typically benign tumors whose growth may induce significant lower cranial nerve deficits. While most tumors arise sporadically, a significant portion is associated with defined genetic syndromes. While surgical resection has historically been the gold standard, management strategies have evolved with acknowledgement of high surgical morbidity, slow tumor growth rates, and technological advances. Conservative management approaches via observation and newer radiation therapy techniques have become more common. This review seeks to provide an update on contemporary management strategies for HNPGLs and future directions.
Genistein induces endocrine resistance in human breast cancer by suppressing H3K27 trimethylation
Authors: Hu Chunyan , Wang Manli , Hu Miao , Ma Shanshan , Yang Bingmo , Xiao Wei , Zhou Qian , Zhou Ming , Li Zhong
Published: November 2022

Genistein (GE), the most important phytoestrogen in diet, is known to behave as a partial agonist of estrogen receptor α and shows a proliferative effect on the growth of breast cancer cell lines. Recent research has reported that long-term consumption of low doses of GE results in hormone-independent growth phenotypes of MCF-7 tumors, with increased HER2. Overexpression of HER2 has been associated with endocrine resistance in human breast cancer, but whether long-term low-level GE-induced HER2 expression is the cause of endocrine resistance remains to be determined. Short-term and long-term treatments with GE may have different effects on HER2 expression. We found that low doses of GE had estrogen-like effects and inhibited HER2 expression after short-term exposure in estrogen receptor-positive breast cancers cells. However, in contrast to short-term exposure, long-term exposure induced an increase in HER2 expression, which led to endocrine resistance. During long-term low-level exposure, the continuous activation of ERK1/2-phosphorylated EZH2 at Ser21 resulted in a decrease of lysine 27 trimethylation. As H3K27me3 levels decreased, the expression of interleukin-6 (IL-6) and IL-8 increased, and HER2 levels gradually increased, forming a feedback loop of ERK1/2/EZH2/IL-6 and IL-8/HER2. We identified a novel pathway by which EZH2 phosphorylation contributed to long-term low-level GE-induced HER2 overexpression and provided new insight for long-term low-level GE-induced acquired endocrine resistance. For breast cancer patients, long-term low-level use of soy supplements has potential health risks, and monitoring dietary exposure to GE is advisable when patients are treated with tamoxifen.
Efecto de la inclusión en la dieta de hidrolizado proteico de pescado sobre el crecimiento corporal y composición proximal del músculo de doncella (Pseudoplatystoma punctifer)
Authors: Cortegano Carlos , Núñez Montúfar Rudy Leandro , Wong Bardales Deykie Max , Prada Rojas Daniel , Villanueva Chávez César Augusto , Contreras Salazar Guadalupe Victoria , Bezada Quintana Sandra Gracia , Carcelén Cáceres Fernando Demetrio , López Guerra Sofía
Published: November 2022

Se evaluó el efecto de la inclusión en la dieta de hidrolizado proteico de pescado sobre el crecimiento corporal y la composición química del músculo de juveniles de doncella.  El estudio siguió un diseño experimental completamente al azar con un arreglo factorial de 2 x 4 [2 dietas vs. 4 tiempos de muestreo (15°, 30°, 45° y 60° día)], resultando en 8 tratamiento con 3 repeticiones cada uno. Los tratamientos fueron: T1: DT15; T2: DHP15; T3: DT30;  T4: DHP30; T5: DT45; T6: DHP45; T7: DT60 y T8: DHP60. Las dietas formuladas fueron isocalóricas (42.15% PB) e isoproteicas (4,415.69 kcal EB kg-1): una testigo (DT; sin suplementación) y otra con inclusión de 1.20% de hidrolizado proteico de pescado (DHP) en substitución de la harina de pescado. Un total de 114 doncellas (642.65 g; 47.93 cm) fueron distribuidas en estanques excavados de 200 m2 (19 peces/estanque) y alimentadas con las dietas experimentales durante 60 días. Cada 15 días, ocho peces por unidad experimental fueron capturados para evaluar el crecimiento corporal de los animales (peso final PF, longitud final LF, ganancia de peso GP, ganancia de longitud GL, tasa de crecimiento específico TCE, conversión alimenticia CA, tasa de eficiencia proteica TEP, sobrevivencia S y factor de condición FC). Adicionalmente, al iniciar (día 0) y finalizar (día 60) el experimento tres peces por unidad experimental fueron sacrificados por punción cerebral para análisis de composición química del músculo. DHP no afectó la LT, GL, TEP, S, FC, ni la composición proximal del músculo de doncella; sin embargo, juveniles de esta especie alimentados con dieta suplementada presentaron mejor desempeño expresados en PF (1049.17 g vs. 919.56 g), GP (406.52 g vs. 276.91 g)y TCE (0.82 vs. 0.60) a los 60 días de alimentación, así como mejor CA en comparación con la dieta sin suplementación.
Características textiles de la fibra de alpacas Huacaya en comunidades altoandinas de la región Tacna, Perú
Authors: Gandarillas Espezua Daniel , Quispe Quispe Abel Eleazar , Puma Iquise Angelina , Torres Hualla Edith Annie , Rios Bobadilla Rosario Milagros , Quispe Coaquira Jesús E.
Published: November 2022

El estudio se llevó en dos comunidades de la Región Tacna, Perú, con el objetivo de determinar las características textiles de fibra de alpacas Huacaya y su grado de asociación con el sexo, edad y color de manto. Se analizaron las muestras de fibra de 817 y 683 alpacas de las comunidades de Huaytire y Maure, respectivamente, con el equipo OFDA 2000. El diseño experimental correspondió a un bloque completo al azar 2x5x2 de efectos fijos. Las fibras de las alpacas de la comunidad de Huaytire presentan fibras más finas (20.51±2.52 µm), con menor dispersión (5.15±0.96 µm), con mayor confort (93.85±8.26 %), y con mayor índice de curvatura (36.58±5.79 °/mm), así como mayor longitud de mecha (10.45±2.21 cm). Las fibras blancas tienen mayor finura (20.79±2.62 µm), y menor dispersión (5.18±0.95 µm) que los de color, en tanto que el factor de confort, índice de curvatura y longitud de mecha de las fibras blancas fueron mayores. Con relación al grupo etario, las alpacas jóvenes presentaron mejores características textiles que las adultas, en tanto solo la finura mostró diferencias significativas (p<0.05) a favor del macho. La longitud de mecha no mostró asociación con las otras características textiles. En conclusión, las características textiles varían de acuerdo con la comunidad, sexo, edad y color de manto, donde los mejores resultados se encontraron en la comunidad de Huaytire, en machos, en fibras blancas y en animales jóvenes.
Structural proteins in the mechanisms of betacoronavirus virion assembly
Authors:   National University of Life and Environmental Sciences of Ukraine, Kyiv, Zaloilo I. , Zaloilo О. ,   Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv;, Rud Y. ,   ESC Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, Ukraine;, Buchatskyi L. ,   ESC Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, Ukraine;
Published: November 2022

The emergence of SARS-CoV-2 caused an urgent need to investigate the molecular mechanisms of its reproduction. However, the detailed step-by-step mechanism of SARS-CoV-2 virion assembly has not been described yet. In the presented review the data on the role of structural proteins in the efficient assembly of betacoronavirus particles are analyzed.
Inhibition of plasma membrane Сa(2+),Mg(2+)-АТРase by сalixarene sulfonylamidines. Structure-activity relationship
Authors:   Muscle Biochemistry Department, Palladin Institute of Biochemistry,National Academy of Sciences of Ukraine, Kyiv, Shkrabak O. A. , Veklich T. O. ,   Muscle Biochemistry Department, Palladin Institute of Biochemistry,National Academy of Sciences of Ukraine, Kyiv;, Rodik R. V. ,   Phosphoranes Chemistry Department, Institute of Organic Chemistry,National Academy of Sciences of Ukraine, Kyiv, Kalchenko V. I. ,   Phosphoranes Chemistry Department, Institute of Organic Chemistry,National Academy of Sciences of Ukraine, Kyiv, Kosterin S. O. ,   Muscle Biochemistry Department, Palladin Institute of Biochemistry,National Academy of Sciences of Ukraine, Kyiv;
Published: November 2022

Previously we have already shown that tetrasulfonylamidinecalixarene C-90 inhibited plasma membrane Са2+,Mg2+-АТРаse of smooth muscle cells selectively to other ATPases of plasma membrane. To inhance the inhibitory effect of calixarenes several alkoxycalixarene sulfonylamidines structurally similar to calixa­rene C-90 were synthesized and their effects on the mentioned enzyme activity, the level of cytoplasmic Ca2+ concentration and hydrodynamic diameter of isolated smooth muscle cells were checked. It was shown that sulfonylamidino groups are crucial for Са2+,Mg2+-АТРаse inhibition, the efficiency of inhibition depends on their quantity and spatial orientation at the upper rim of calixarene macrocycle. Introduction of phenyl or tert-butyl groups into the upper rim and of long alkyl chains into the lower rim led to only slightl increase of inhibition efficiency. The inhibitory effect of studied calixarenes on Са2+,Mg2+-АТРаse correlated with effects on cytosolic Ca2+ concentration and hydrodynamic diameter of smooth muscle cells. The obtained results are important for creation of more effective and selective inhibitors of plasma membrane Са2+,Mg2+-АТРаse as regulators of smooth muscle contractility. Keywords: calixa­rene sulfonylamidines, intracellular Ca(2+) concentration, plasma membrane Са(2+);Mg(2+)-АТРаse, smooth muscle
Localization and level of proapoptotic protein regulators in a rat lung tissue during development of acute experimental bronchopneumonia
Authors:   Bogomolets National Medical University, Kyiv, Ukraine, Ziablitsev D. S. , Tykhomyrov A. O. ,   Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;, Dyadyk O. O. ,   Shupyk National Healthcare University of Ukraine, Kyiv;, Kolesnikova S. V. ,   Bogomolets National Medical University, Kyiv, Ukraine;, Ziablitsev S. V. ,   Bogomolets National Medical University, Kyiv, Ukraine;
Published: November 2022

Apoptosis plays an important role in the development of acute inflammatory lung injury (AILI) and its consequences, which can be realized in different cells with distinct intensity and rate. The aim of this study was to determine the distribution and expression intensity of apoptosis markers in the lungs of rats in the AILI model with endotracheal introduction of capron thread and LPS. Immunoblotting and immunohistochemical studies were performed using monoclonal antibodies against Bax and caspase-3 proteins. It was shown that Bax level increased significantly with the peak on the 7th day. The second peak of Bax 40 dimeric form was noted on the 21st day. The level of both pro-caspase-3 and active caspase-3 was also dramatically increased with a maximum on the 5th day and the second peak of active caspase-3 content was observed on the 21st day. These changes reflected the activation of apoptosis in key trigger periods of AILI during the development of exudative hemorrhagic pneumonia and subsequent fibrotic remodeling of the lungs. Keywords: AILI, apoptosis, Bax, caspase-3, lipopolysaccharide