bluuebunny/crossref_metadata_2025 huggingface dataset:
Mild traumatic brain injury is associated with effect of inflammation on structural changes of default mode network in those developing chronic pain
Authors: Niu Xuan , Bai Lijun , Sun Yingxiang , Wang Yuan , Bai Guanghui , Yin Bo , Wang Shan , Gan Shuoqiu , Jia Xiaoyan , Liu Hongjuan
Published: October 2020

Background：Mild traumatic brain injury (mTBI) has a higher prevalence (more than 50%) of developing chronic posttraumatic headache (CPTH) compared with moderate or severe TBI. However, the underlying neural mechanism for CPTH remains unclear. This study aimed to investigate the inflammation level and cortical volume changes in patients with acute PTH (APTH) and further examine their potential in identifying patients who finally developed CPTH at follow-up.Methods：77 mTBI patients initially underwent neuropsychological measurements, 9-plex panel of serum cytokines and MRI scans within 7 days post-injury (T-1) and 54(70.1%) of patients completed the same protocol at a 3-month follow-up (T-2). 42 matched healthy controls completed the same protocol at T-1 once. Results：At baseline, mTBI patients with APTH presented significantly increased GM volume mainly in the right dorsal anterior cingulate cortex (dACC) and dorsal posterior cingulate cortex (dPCC), of which the dPCC volume can predict much worse impact of headache on patients’ lives by HIT-6 (β = 0.389, P = 0.007) in acute stage. Serum levels of C-C motif chemokine ligand 2 (CCL2) were also elevated in these patients, and its effect on the impact of headache on quality of life was partially mediated by the dPCC volume (mean [SE] indirect effect, 0.088 [0.0462], 95% CI, 0.01-0.164). Longitudinal analysis showed that the dACC and dPCC volumes as well as CCL2 levels had persistently increased in patients developing CPTH 3 months postinjury. Conclusion：The findings suggested that structural remodelling of DMN brain regions were involved in the progression from acute to chronic PTH following mTBI, which also mediated the effect of inflammation processes on pain modulation.Trial registration: ClinicalTrial.gov ID: NCT02868684; registered 16 August 2016.
Surgical Treatment for Anomalous Origin of the Right Coronary Artery from the Pulmonary Artery: A Case Report with Five-Year Follow-up
Authors: Teng Peng , Li Weidong , Ni Yiming
Published: October 2020

Background Anomalous origin of the right coronary artery from the pulmonary artery (ARCAPA) is a rare congenital heart disease affecting about 0.002% of the population. Knowledge of ARCAPA is almost collected from case reports. The aim of this study was to provide a rare case to better understand this rare congenital coronary anomaly. Case presentation We report a rare case of an 18-year-old male who was initially referred because of heart murmur. Dilated and tortuous coronary arteries were detected by echocardiography and congenital coronary anomaly was suggested. Further coronary CT angiography confirmed the diagnosis of ARCAPA. Although dual coronary system provides favorable long-term outcome, bypass surgery was considered technically difficult due to the huge mismatch of caliber between the right coronary artery and graft vessels. Eventually, simple right coronary artery ligation was performed. The patient was followed up for about 5 years without evidence of atherosclerosis or myocardial ischemia.Conclusions ARCAPA presents as a rare congenital heart disease with variable clinical manifestations. Surgical treatment is highly recommended to re-establish dual coronary system and prevent further complications. To our best knowledge, only about 200 cases of ARCAPA has been reported.
Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)
Authors: Cao Yi-Fan , Chen Hui-Xia , Li Yang , Zhou Dang-Wei , Chen Shi-Long , Li Liang
Published: October 2020

Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites from the Tibetan antelope remains limited. Methods: Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences [i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1)] were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes.Results: Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum sp. n. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions: A new species of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum sp. n. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.
Recovery of preoperative absolute knee extension and flexion strength after ACL reconstruction
Authors: Piussi Ramana , Broman Daniel , Musslinder Erik , Beischer Susanne , Thomée Roland , Senorski Eric Hamrin
Published: October 2020

BackgroundThe recovery of muscle function after an Anterior Cruciate Ligament (ACL) reconstruction is most commonly reported as limb-to-limb differences using the Limb Symmetry Index (LSI), which is not free from limitations. The purpose of this study was to compare the proportion of patients who recover their Preoperative Absolute Muscle Strength (PAMS) 8 and 12 months after ACL reconstruction with the proportion of patients who recover their symmetrical knee strength. A secondary aim was to assess the relationship between psychological Patient-Reported Outcomes (PROs) and recovering PAMS at 8 and 12 months after ACL reconstruction and rehabilitation.MethodPreoperative, 8- and 12-month results from quadriceps and hamstring strength tests and PROs for 117 patients were extracted from a rehabilitation registry. Individual preoperative peak torques from strength tests were compared with results from the 8- and 12-month follow-ups respectively. Patients were defined as having recovered their PAMS upon reaching 90% of their preoperative peak torque for both quadriceps and hamstring strength. Patients were defined as having recovered their LSI upon reaching a value ≥ 90% when comparing the results for their injured knee with those of their healthy knee. Correlations between the recovery of PAMS and PROs at 8 and 12 months were analyzed.ResultsThere was no difference in the proportion of patients who recovered their PAMS compared with patients who recovered their LSI. In all, 30% and 32% of the patients who recovered their LSI had not recovered their PAMS at 8 months and 12 months respectively. In the patients who had recovered their PAMS, 24% and 31% had not recovered their symmetrical LSI at 8 months and 12 months respectively. There was no significant correlation between the recovery of PAMS and psychological PROs. ConclusionThe use of both PAMS and LSI provides more detailed information on the recovery of muscle strength after ACL reconstruction. The recovery of PAMS was not correlated with psychological traits, which implies that both PROs and PAMS are important when evaluating patients after ACL reconstruction.Trial RegistrationEthical approval has been obtained from the Regional Ethical Review Board in Gothenburg, Sweden (registration numbers: 265-13, T023-17).
Changes in Serum Homocysteine Concentrations and their Prognostic Value in Pregnant Chinese Women with Preeclampsia : A Retrospective Study
Authors: Qin Wanyuan , Hu Xuehua , Fu Chunyun , Tang Yuqun , Lu Xiangjun , Deng Zuoxin , Huang Qiang , Wei Xiaofen , Chen Yan , Wang Junlong , Huang Kangkai , Jing Junpeng
Published: October 2020

Background: This study evaluated whether changes in homocysteine concentrations in pregnant women with preeclampsia might be useful for predicting pregnancy-related outcomes.Methods: This retrospective study evaluated 2,745 pregnant Chinese women (1,368 women with preeclampsia and 1377 healthy pregnant controls) at two Chinese centres. Medical records were reviewed to collect data regarding maternal age, homocysteine concentrations, gestational weight gain, body mass index (BMI), neonatal weight, Apgar score, previous abortions, previous deliveries, adverse pregnancy history, and pregnancy-related outcomes.Results: Maternal serum homocysteine concentrations were significantly higher in the preeclampsia group (vs. the control group) during the second trimester (P<0.0001) and the third trimester (P<0.0001). Foetal death was also significantly more common in the preeclampsia group. Significant differences (P<0.05) were also observed between the preeclampsia and control groups in terms of maternal age, gestational weight gain, BMI, neonatal weight, Apgar score, previous abortions, previous deliveries, gestation length, adverse pregnancy history, and pregnancy outcomes. Women with preeclampsia had elevated homocysteine concentrations that increased from the first trimester until the third trimester. Among women with preeclampsia, the risk of foetal death was significantly associated with maternal age, BMI, neonatal weight, Apgar score, and serum homocysteine concentrations during the second trimester (P=0.022) and the third trimester (P=0.034). Conclusion: Our results indicate that foetal death in women with preeclampsia was associated with maternal age, BMI, neonatal weight, and Apgar score. Furthermore, foetal death was associated with high maternal homocysteine concentrations during the second and third trimesters.
TNFAIP3 Suppresses Lung Cancer Progression via Regulating Cell Proliferation and Apoptosis
Authors: Chen Han , Gao Jie , Yu Yongsheng , Zhou Qian , yongqi Shan
Published: October 2020

Background: The ubiquitin-editing enzyme TNF inducible protein 3 (TNFAIP3) is a crucial regulator of inflammation and immunity. It is also involved in tumorigenesis of various cancers such as lymphomas, colorectal tumors and breast cancer. In this study, we aimed to explore the role and regulatory mechanism of TNFAIP3 in lung cancer. Methods: The expression of TNFAIP3 was determined in the Cancer Genome Atlas (TCGA) database. The levels of TNFAIP3 in lung cancer tissues was determined by immunohistochemistry (IHC) assay. TNFAIP3 knockdown and overexpression were performed, followed by further evaluation of cell viability, cell cycle and apoptosis. Cell cycle and apoptosis were observed by using flow cytometry and the key regulatory proteins were detected by western blotting. Colony formation assessment and EdU assay were adopted to check cell proliferation. Results: TNFAIP3 expression was downregulated in lung cancer tissues at both mRNA and protein levels, comparing with that in adjacent non-tumor tissues. Consequently, the colony formation ability of lung cancer cells was enhanced, and the number of EdU positive lung cancer cells was increased. By contrast, elevated TNFAIP3 expression resulted in decreased colony formation ability of lung cancer cells. Mechanistically, TNFAIP3 overexpression rendered cell cycle of lung cancer cells halted at G0/G1 phase and caused apoptosis of lung cancer cells.Conclusion: Our data suggested that TNFAIP3 exhibits tumor suppressive roles in lung cancer.
Metabolome and Transcriptome Analysis of Flavor Components and Flavonoid Biosynthesis in Fig Fruit (Ficus Carica L.) After Bagging
Authors: Wang Ziran , Song Miaoyu , Wang Zhe , Chen Shangwu , Ma Huiqin
Published: October 2020

Background: Bagging can improve the appearance of fruits and increase the food safety and commodification, it also has effects on intrinsic quality of the fruits, which was commonly reported negative changes. Fig can be regarded as a new model fruit with its relatively small genome size and long fruit season. Results: In this study, widely targeted metabolomics based on HPLC MS/MS and RNA-seq of the fruit tissue of the ‘Zibao’ fig before and after bagging were analyzed to reveal the metabolites changes of the edible part of figs and the underneath gene expression network changes. A total of 771 metabolites were identified in the metabolome analysis using fig female flower tissue. Of these, 88 metabolites (including one carbohydrate, eight organic acids, seven amino acids, and two vitamins) showed significant differences in fruit tissue before and after bagging. Changes in 16 structural genes, 13 MYB transcription factors, and endogenous hormone (ABA, IAA, and GA) metabolism and signal transduction-related genes in the biosynthesis pathway of flavonoids after bagging were analyzed by transcriptome analysis. KEGG enrichment analysis also determined significant differences in flavonoid biosynthesis pathways in female flower tissue before and after bagging. Conclusions: This work provided comprehensive information on the composition and abundance of metabolites in the female flower tissue of fig. The results showed that the differences in flavor components of the fruit before and after bagging could be explained by changes in the composition and abundance of carbohydrates, organic acids, amino acids, and phenolic compounds. This study provides new insights into the effects of bagging on changes in the intrinsic and appearance quality of fruit.
Exploring the Mechanisms Underlying the Therapeutic Effect of Salvia Miltiorrhiza Against Diabetic Nephropathy Using Network Pharmacology and Molecular Docking
Authors: Zhang Li-Li , Han Lin , Wang Xin-Miao , Wei Yu , Zheng Jing-Hui , Zhao Lin-Hua , Tong Xiaolin
Published: October 2020

BackgroundThe mechanisms underlying the therapeutic effect of Salvia Miltiorrhiza (SM) against diabetic nephropathy (DN) using systematic network pharmacology and molecular docking methods were examined.MethodsTCMSP database was used to screen the active ingredients of SM. Gene targets were obtained using Swiss Target Prediction and TCMSP databases. Related targets of DN were retrieved from the Genecards and DisGeNET databases. Next, a PPI network was constructed using the common targets of SM-DN in the STRING database. The Metascape platform was used for GO function analysis and Cytoscape plug-in ClueGO was used for KEGG pathway enrichment analysis. Molecular docking was performed using iGEMDOCK and AutoDock Vina software. Pymol and LigPlos were used for mapping the network. ResultsSixty-six active ingredients and 189 targets were screened from SM. Among them, 64 targets overlapped with DN targets. The PPI network diagram revealed that AKT1, VEGFA, IL6, TNF, MAPK1, TP53, EGFR, STAT3, MAPK14, and JUN were the top 10 relevant targets. GO and KEGG analyses mainly focused on advanced glycation end products, oxidative stress, inflammatory response, and immune regulation. Molecular docking revealed that the potential target genes closely related to DN, including TNF, NOS2, and AKT1, were more stable in combination with salvianolic acid B, and their stability was better than that of tanshinone IIA.ConclusionThis study reveals the active components and potential molecular mechanisms involved in the therapeutic effect of SM against DN and provides a reference for the wide application of SM in clinically managing DN.
The Mechanism of Aidi Injection on Breast Cancer Based on Network Pharmacologyanalysis
Authors: Su Jing , Liu Kedi , Tao Xingru , Li Fei , Zhao Shi , Bai Yang , Lu Xinming , Li Jing , Chen Sha , Duan Jialin , Wei Peifeng , xi Miaomiao
Published: October 2020

Background: Aidi injection (ADI)is a Chinese patent medicine with anti-cancer effect, which has been used to treat breast cancer (BC) in China for many years, but its potential pharmacological mechanism is still indeterminacy. In this resaearch, network pharmacology, a systematic and comprehensive approach, was used to reveal ADI's potential pharmacological mechanism in treating BC for the first time.Methods: Databases were used to collect targets related to the bioactive components of ADI and BC. the relevant networks were established by the string database, and were screened potential bioactive components and core targets. Eventually, core targets and pathway enrichment were analyzed by DAVID database.Results: As the results showed, we collected 99 bioactive ingredients,345 ADI-related targets after deduplication and 368 BC-related targets. Of these, 108 common targets were overlapped. We then performed an enrichment analysis on the common target network and the protein-protein interaction (PPI) network.Conclusion: The results showed that ADI may inhibit breast cancer through seven important signal pathways involved in the "regulation of vascular endothelial function", "inflammatory response" and "apoptosis” biological processes. Through further clustering and enrichment analysis of the PPI network of ADI’s bioactive component targets and BC-related targets, we found that cancer, ErbB, MAPK, TLR, chemokine, p53 and cell cycle signaling pathway, mainly contributed to the effects of ADI in treating BC. In conclusion, this study reveals the possible mechanism of ADI in treating BC, and provides a new direction for drug development for ADI in treating BC.
Long noncoding RNA Gm44593 attenuates oxidative stress from age-related hearing loss by regulating miR-29b/WNK1
Authors: Li Qian , Zang Yanzi , Sun Zhanwei , Zhang Wenqi , Liu Hongjian
Published: October 2020

AmisLong noncoding RNA has been reported to play important role in various disease. However, the function of lncRNA in age-related hearing loss still unclear. The aim of our study is to investigate the function and mechanism of lncRNA Gm44593 in AHLMaterials and methodsATP content, JC-1 assay, mitochondrial content, cell death rates and dual-luciferase reporter assay were performed to assess the function of lncRNA Gm44593 in HEI-OC1 cells and the mouse cochlea.ResultsThe expression of lncRNA Gm44593 was significantly upregulated upon H2O2 and starvation treatment. Overexpression of lncRNA Gm44593 manifestly reduced the cell death rates. The ATP content, mtDNA content and mitochondrial membrane potential were alleviated upon overexpression of lncRNA Gm44593. We also proved that miR-29b is the direct target of lncRNA Gm44593. Overexpression of miR-29b completely restored the effect induced by lncRNA Gm44593. In addition, we provided evidences that WNK1 is the direct target of miR-29b.Conclusionour research uncovers the role of lncRNA Gm44593 in age-related hearing loss. We provide new insights into potential therapeutic targets for the amelioration of age-related hearing loss.
SSACBKM: An Integration Model for Biomedical Relationship Extraction
Authors: Shi Huiping , Zhou Xiaobing
Published: October 2020

Background: The cause of the disease is one of the main contents of biomedical research. Extracting effective relational information from a large number of biomedical texts has important applications for biomedical research. At present, most of the work of biomedicine is to use manual screening or use rule-based or feature-based pipeline network models to obtain screening characteristics. These methods require a lot of time to design specific rules or features to complete specific tasks, resulting in some features that non-compliant features cannot be filtered out.Results: The model gets micro-F1 scores of 0.802 and 0.876 on the Chemprot data set and DDI data set, respectively. The resources that can be used in this project can be found in https://github.com/HunterHeidy/DDICPI-.Conclusions: Experiments have proved that without Bert, you can get good results by learning from Bert<s core ideas.
Atypical Resting-State Functional Connectivity of Intra/Inter-Sensory Networks is Related to Symptom Severity in Young Boys with Autism Spectrum Disorder
Authors: Wang Jia , Wang Xiaomin , Wang Runshi , Duan Xujun , Chen Heng , He Changchun , Zhai Jinhe , Chen Huafu , Wu Lijie
Published: October 2020

Background Autism spectrum disorder (ASD), a neurodevelopmental disorder, has been reported with an altered brain connectivity pattern in the sensory network using resting-state functional magnetic imaging (rs-fMRI) compared to typical developing participants (TDs). However, there is still no consistent conclusion. In the current study, we investigated the alterations of the intra-network and inter-network connectivity pattern relating to sensory in children with ASD compared with TDs, and further assessed whether these alterations are associated with autistic behavioral symptoms. Methods rs-fMRI was used to assess young boys with ASD (N=29) and TD (N=29), aged 3-7-years. Groups were matched for age and handiness. Spatial patterns of the sensory rest state networks (RSNs) were obtained using group Independent component analysis (ICA) method, and between-groups differences were evaluated within each sensory network. Then, the time series of each RSN were extracted from each participant preprocessed data. Correlation analysis was assessed among intra- and inter-network functional connectivity (FC) and symptom severity in children with ASD. Results Four sensory components were identified, including auditory network (AN), higher visual network (HVN), primary visual network (PVN) and sensorimotor network (SMN). Functional images revealed two sensory networks exhibiting significant increased FCs in ASD group, located within AN and SMN. Higher positive connectivity between PVN and HVN in ASD group is associated with symptom severity. Conclusion Current study might shed light that the abnormal connectivity patterns of sensory network regions may underlie impaired higher-order multisensory integration in ASD children, and social impairment of ASD are caused probably by aberrant FCs involving inter/intra- sensory network.
Botulinum Toxin Injections as an Effective Treatment for Patients with Hailey-Hailey or Darier Disease: An open-label 6-month pilot interventional study
Authors: Dreyfus Isabelle , Maza Aude , Rodriguez Lauriane , Merlos Margot , Texier Hélène , Rousseau Vanessa , Sommet Agnès , Mazereeuw-Hautier Juliette
Published: October 2020

Background: Patients with Hailey-Hailey and Darier diseases present with disabling inflammatory lesions located in large skin folds, which are often exacerbated or induced by sweating. Quality of life is highly impaired because of pain and recurrent skin infections. An improvement in skin lesions after botulinum toxin A injections has previously been reported in some patients but no prospective interventional studies are available. The aim of this open-label, 6-month, interventional pilot study (NCT02782702) was to evaluate the effectiveness and safety of botulinum toxin A injections. Thirty patients with moderate to very severe skin lesions located in folds were enrolled in the study. Results: Thirty patients (26 Hailey-Hailey / 4 Darier) were included. Botulinum toxin A proved effective within the first month in two-thirds of patients, taking all study parameters (itchiness, cutaneous pain, sweating and odour, infections, psychosocial impairment and quality of life) into account and persisted during the 6-month follow-up period. No patient was classed as a BtxA non-responder, but 11 (37%) patients, who were the most severe ones, experienced a relapse during the study. No serious side effects were reported. Mild transient clear fluid discharge at the site of the injections was reported for 27% of patients. Conclusions: Botulinic toxin is an effective and safe treatment for Hailey-Hailey and Darier diseases. Further studies should evaluate the benefit of increased doses or combined treatments for the most severe patients.Timing of approvals:- 25, February 2015: approval by the institutional ethics committee- 2, June 2015: registration under the European Eudract identifier 2015-000379-29 (https://eudract.ema.europa.eu/index.html)- 31, July 2015: approval by the French national health authority, ANSM - 31, August 2015: implementation agreement signed by the sponsor. - 25, May 2016: registration under the ClinicalTrials.gov identifier NCT02782702 (https://clinicaltrials.gov/ct2/show/NCT02782702?cond=NCT02782702&draw=2&rank=1).
Time-dependent responses of the neutral mass density to magnetospheric energy inputs into the cusp region in the thermosphere: A high-resolution two-dimensional local modeling
Authors: Oigawa Tomokazu , Shinagawa Hiroyuki , Taguchi Satochi
Published: October 2020

Remarkable enhancements of the thermospheric mass density around the 400-km altitude in the cusp region have been observed by the CHAllenging Minisatellite Payload (CHAMP) satellite. We employed a high-resolution two-dimensional local model to gain insights into the extent to which the neutral-ion drag process controls the mass density’s enhancements under the energy inputs typical of the cusp. We expressed those energy inputs by quasi-static electric fields and electron precipitation. We compared two cases and calculated the thermospheric dynamics with and without neutral-ion drags. We found that in the more realistic case containing the neutral-ion drag, the calculated mass density enhancement was 10% at most, which is dramatically smaller than the observations by the CHAMP satellite (33% on average). The results also showed that the neutral-ion drag process suppresses Joule heating and neutral mass density enhancements, as well as the chemical reaction process. The discrepancy between our modeling result and the satellite observation suggests the existence of additional energy sources, such as Alfvén waves propagating from the magnetosphere, which play an important role in the cusp’s density enhancement.
Volcanic Unrest at Hakone Volcano after the 2015 phreatic eruption — Reactivation of a Ruptured Hydrothermal System?
Authors: Mannen Kazutaka , Abe Yuki , Daita Yasushi , Doke Ryosuke , Harada Masatake , Kikugawa George , Honma Naoki , Miyashita Yuji , Yukutake Yohei
Published: October 2020

Since the beginning of the 21st century, volcanic unrest has occurred every 2–5 years at Hakone volcano. After the 2015 eruption, unrest activity changed significantly in terms of seismicity and geochemistry. In this paper, characteristics of the post-eruptive volcanic unrest that occurred in 2017 and 2019 are described, and changes in the hydrothermal system of the volcano caused by the eruption are discussed. Like the pre- and co-eruptive unrest, each post-eruptive unrest episode was detected by deep inflation below the volcano (~ 10 km) and deep low frequency events, which can be interpreted as reflecting supply of magma or magmatic fluid from depth. The seismic activity during the post-eruptive unrest episodes also increased; however, seismic activity beneath the eruption center during the unrest episodes was significantly lower, especially in the shallow region (~2 km), while sporadic seismic swarms were observed beneath the caldera rim, ~3 km away from the center. The 2015 eruption established routes for steam from the hydrothermal system (≥ 150 m deep) to the surface through the cap-rock, allowing emission of super-heated steam (~ 160 ºC), which was absent before the eruption. This steam showed an increase in magmatic/hydrothermal gas ratios (SO2/H2S and HCl/H2S) in the 2019 unrest, which may be interpreted as magmatic intrusion at shallow depth; however, no indicative seismic and geodetic signals were observed. Net SO2 emission during the post-eruptive unrest episodes, which remained within the usual range of the post-eruptive period, is also inconsistent with shallow intrusion. We consider that the post-eruptive unrest episodes were also triggered by newly derived magma or magmatic fluid from depth; however, the breached cap-rock was unable to allow subsequent pressurization of the hydrothermal system beneath the volcano center and suppressed seismic activity significantly. The heat released from the newly derived magma or fluid dried the vapor-dominated portion of the hydrothermal system and inhibited scrubbing of SO2 and HCl to allow a higher magmatic/hydrothermal gas ratio. The 2015 eruption could have also breached the sealing zone near the brittle–plastic transition and the subsequent self-sealing process seems not to have completed based on the observations during the post-eruptive unrest episodes.
“…the availability of contraceptives is everywhere.”: Coordinated and Integrated Family Planning Service Delivery in Rwanda
Authors: Scanteianu Adriana , Schwandt Hilary M. , Boulware Angel , Corey Julia , Herrera Ana , Hudler Ethan , Imbabazi Claudette , King Ilia , Linus Jessica , Manzi Innocent , Merrit Maddie , Mezier Lyn , Miller Abigail , Morris Haley , Musemakweli Dieudonne , Musekura Uwase , Mutuyimana Divine , Ntakarutimana Chimene , Patel Nirali , Shemeza Biganette-Evidente , Sterling-Donaldson Gi’anna , Umutoni Chantal , Uwera Lyse , Zeiler Madeleine , Feinberg Seth
Published: October 2020

BackgroundContraceptive use in Rwanda tripled since 2005. This study aims to understand the role of coordinated and integrated family planning service delivery in achieving this increase in contraceptive use in Rwanda.MethodsThis qualitative study in 2018 included eight focus group discussions with family planning providers and 32 in-depth interviews with experienced family planning users.ResultsResults indicate a well-coordinated family planning service delivery system with community health workers and nurses filling different and complementary roles in meeting family planning client needs at the local level. In addition, integration of family planning into other maternal and child health services is the norm.ConclusionsThe coordination and integration of family planning across both providers and services may help explain the rapid increase in Rwanda’s contraceptive use and has potential applications for enhancing family planning service delivery in other settings.
Biofilm Microbiome in Extracorporeal Membrane Oxygenator Catheters
Authors: Yu Yeuni , Kim Yun Hak , Cho Woo Hyun , YEO HYE JU
Published: October 2020

Background : To assess the relationship between bacteremia and biofilms in extracorporeal membrane oxygenation (ECMO) catheters.Methods: We evaluated the biofilm microbiome of ECMO catheters from adults with (n = 6) and without bacteremia (n = 15). Microbiomes of the catheter biofilms were evaluated by profiling the V3 and V4 regions of bacterial 16s rRNA genes using the Illumina Miseq sequencing platform.Results: In total, 2,548,172 reads, with an average of 121,341 reads per sample, were generated. Although alpha diversity was slightly higher in the non-bacteremic group, it was not statistically significant. Also, there was no difference in beta diversity between the two groups. At the genus level, Delftia was more abundant in the non-bacteremic group, but Bacillus, Flavobacterium, CL0-1, Candidatus, and Xiphinematobacter were more abundant in the bacteremic group. In particular, Arthrobacter, SMB53, Neisseria, Candidatus, Ortrobactrum, Candidatus, Rhabdochlamydia, Deefgae, Dyella, Paracoccus, and Pedobacter were more abundant in the bacteremic group. In a network analysis, compared to the non-bacteremic group, the microbiome of the bacteremic group was very complex. Notably, there was a significant elevation in the secretion system of the non-bacteremic group.Conclusions: Biofilm characteristics in ECMO catheters varied according to the presence or absence of bacteremia.
WITHDRAWN: The Negative Impact of Obesity on the Occurrence and Prognosis of the 2019 Novel Coronavirus (COVID-19) Disease: A Systematic Review and Meta-Analysis
Authors: Raeisi Tahereh , Mozaffari Hadis , Sepehri Nazaninzahra , Alizadeh Mohammad , Darand Mina , Razi Bahman , alizadeh Shahab
Published: October 2020

Background: the 2019 novel coronavirus (COVID-19) is an emerging pandemic, with a disease course varying from asymptomatic infection to critical disease resulting to death. Recognition of prognostic factors is essential because of its growing prevalence and high clinical costs. This meta-analysis aimed to evaluate the global prevalence of obesity in COVID-19 patients and to investigate whether obesity is a risk factor for the COVID-19, COVID-19 severity, and its poor clinical outcomes including hospitalization, intensive care unit (ICU) admission, need for mechanical ventilation, and mortality.Methods: The study protocol was registered on to PROSPERO (CRD42020203386). A systematic search of Scopus, Medline, and Web of Sciences was conducted on June 2020, to find pertinent studies. After selection, 54 studies from 10 different countries were included in the quantitative analyses. Pooled odds ratios (OR) with 95% confidence intervals (CIs) were calculated to assess the associations. Results: The prevalence of obesity was 33% (95% CI, 30.0%–35.0%) among patients with COVID-19. Obesity was significantly associated with susceptibility to COVID-19 (OR=2.42, 95% CI: 1.58 to 3.70; moderate certainty) and COVID-19 severity (OR=1.62, 95% CI: 1.48 to 1.76; low certainty). Furthermore, obesity was a significant risk factor for hospitalization (OR=1.75, 95% CI: 1.47 to 2.09; very low certainty), mechanical ventilation (OR=2.24, 95% CI: 1.70 to 2.94; low certainty), intensive care unit (ICU) admission (OR=1.75, 95% CI: 1.38 to 2.22; low certainty), and death (OR=1.23, 95% CI: 1.06 to 1.41; low certainty) in COVID-19 patients. In the subgroup analyses, these associations were supported by the majority of subgroups. Conclusions: Obesity is associated with COVID-19 and its poor clinical outcomes. Thus, it is highly recommended to consider obesity status in prognostic scores and improvement of guidelines for the clinical care of patients with COVID-19.
Research Trends on High-Flow Nasal Cannula Oxygen Therapy: A Bibliometric Analysis from 2000 to 2019
Authors: Qi Siyi , Zhang Xiao , Chen Sifan , Luo Chengzhun , Dai Wanbing , Zhang Yizhe , Zhang Yuanyuan , Tian Weitian , Chen Xuemei , Gu Xiyao , Yu Weifeng , Tian Jie , Su Diansan
Published: October 2020

Purpose: High-flow nasal cannula oxygen therapy (HFNC) has been increasingly reported over the past decades. Therefore, this study aimed to analyze the evolution of HFNC research and qualitatively and quantitatively evaluate publications. Methods: Publications between 2000 and 2019 were retrieved from the Web of Science Core Collection on August 17, 2020, according to a search strategy. Different kinds of software, CiteSpace and VOSviewer, and an online bibliometric analysis platform were used to identify top authors, journals, institutions, countries, keywords, co-cited articles, and trends. Results: Finally, 835 publications were identified, and the annual number of publications showed an increasing trend. Of these, 273 journals published articles related to HFNC. The United States and the University of Melbourne were the leading country and institution for publications, respectively. Co-cited reference analysis revealed top landmark articles. High-flow nasal cannula, bronchiolitis, hypercapnia, newborn, and immunosuppression are some of the high-frequency keywords in the co-occurrence cluster and co-cited reference cluster analyses, indicating that the clinical application of oxygen therapy remains a hot spot. Burst detection analysis of top keywords showed that randomized controlled trial, viral bronchiolitis, and immunocompromised patient were the new research foci.Conclusions: Bronchopulmonary dysplasia, infant, premature infant, respiratory distress syndrome, critical care patient, preoxygenation, extubation failure, and acute lung injury are the research foci of HFNC over the past 20 years and have emerged as a basis for transformation from infancy to adulthood and from a single disease to diverse diseases.
Development of A Model for Predicting The 4-Year Risk of Systematic Knee Osteoarthritis in China: A Longitudinal Cohort Study
Authors: Wang Limin , Lu Han , Chen Hongbo , Jin Shida , Wang Mengqi , Shang Shaomei
Published: October 2020

Objectives: We aimed to develop a model for predicting the 4-year risk of knee osteoarthritis (KOA) based on survey data obtained via a random, nationwide sample of Chinese individuals.Methods: We analyzed data from 8,193 middle-aged and older adults included in the China Health and Retirement Longitudinal Study (CHARLS). The incident of systematic KOA was defined as participants were free of systematic KOA at baseline (CHARLS2011) and were diagnosed with systematic KOA at the 4-year follow-up (CHARLS2015). We estimated the effects of potential predictors on the incident of KOA using logistic regression models and validated the final model internally. Model performance was assessed based on discrimination (area under the receiver operating characteristic curve, AUC) and calibration. Results: A total of 815 incident cases of KOA were identified at the four-year follow-up, resulting in a cumulative incidence of approximately 9.95%. The final multivariate model included age, sex, waist circumference, residential area, difficulty with activities of daily living (ADLs)/instrumental activities of daily living (IADLs), history of hip fracture, depressive symptoms, number of chronic comorbidities, self-rated health status, and level of moderate physical activity (MPA). The bias-corrected AUC for this model was 0.704. The calibration curve revealed satisfactory agreement between the observed and predicted incidence of systematic KOA. A simple clinical score model was developed for easily quantifying the risk of KOA based on these factors.Conclusion: Our prediction model may aid in the early identification of individuals at the greatest risk of developing KOA within 4 years.
Transcriptome-wide association study identifies susceptibility genes for rheumatoid arthritis
Authors: Wu Cuiyan , Tan Sijia , Liu Li , Cheng Shiqiang , Li Peilin , Li Wenyu , Liu Huan , Zhang Feng'e , Wang Sen , Ning Yujie , Wen Yan , Zhang Feng
Published: October 2020

ObjectiveTo identify rheumatoid arthritis (RA) associated susceptibility genes and pathways through integrating genome-wide association study (GWAS) and gene expression profile data. MethodsA transcriptome-wide association study (TWAS) was conducted by the FUSION software for RA considering EBV-transformed lymphocytes (EL), transformed fibroblasts (TF), peripheral blood (NBL) and whole blood (YBL). GWAS summary data was driven from a large-scale GWAS, involving 5,539 autoantibody-positive RA patients and 20,169 controls. The TWAS-identified genes were further validated using the mRNA expression profiles and made a functional exploration. ResultsTWAS identified 692 genes with PTWAS values < 0.05 for RA. CRIPAK (PEL = 0.01293, PTF = 0.00038, PNBL = 0.02839, PYBL = 0.0978), MUT (PEL = 0.00377, PTF = 0.00076, PNBL = 0.00778, PYBL = 0.00096), FOXRED1 (PEL = 0.03834, PTF = 0.01120, PNBL = 0.01280, PYBL = 0.00583) and EBPL (PEL = 0.00806, PTF = 0.03761, PNBL = 0.03540, PYBL = 0.04254) were collectively expressed in all the four tissues/cells. 18 genes, including ANXA5, AP4B1, ATIC (PTWAS = 0.0113, down-regulated expression), C12orf65, CMAH, PDHB, RUNX3 (PTWAS = 0.0346, down-regulated expression), SBF1, SH2B3, STK38, TMEM43, XPNPEP1, KIAA1530, NUFIP2, PPP2R3C, RAB24, STX6, TLR5 (PTWAS = 0.04665, up-regulated expression), were validated with integrative analysis of TWAS and mRNA expression profiles. TWAS-identified genes functionally involved in endomembrane system organization, endoplasmic reticulum organization, regulation of cytokine production, TNF signaling pathway, etc. ConclusionWe identified multiple candidate genes and pathways, providing novel clues for the genetic mechanism of RA.
The burden of premature ventricular contractions predicts adverse fetal and neonatal outcomes among pregnant women without structural heart disease
Authors: Lin Jing , Qian Yanxia , Wu Xin , Chen Qiushi , Ding Qiang , Zhang Mingming , Wang Junhong
Published: October 2020

Objective: To investigate the outcomes of fetuses or neonates of pregnant women with premature ventricular contractions (PVCs). Study design: 6, 148 pregnant women were prospectively enrolled in the study. Of these women, 103 with a PVC burden >0.5% were divided into two groups based on the presence or absence of adverse fetal or neonatal events. The adverse outcomes were compared between the groups to assess the impact of PVCs on pregnancy. Results: A total of 17 adverse events (12 cases) occurred among 103 pregnant women with PVCs, which was significantly higher than that among women without PVCs (11.65% vs. 2.93%, p<0.01). The median PVC burden among pregnant women with PVCs was 2.84% (1.02% to 6.1%). Furthermore, compared with that of the women without adverse events, the median PVC burden of women with adverse fetal or neonatal outcomes was significantly higher (9.02% vs. 2.30%, p<0.01). Multivariate logistic regression analysis demonstrated that PVC burden was associated with adverse fetal or neonatal outcomes among pregnant women with PVCs (OR: 1.34, 95% CI (1.11-1.61), p<0.01). Conclusions: Frequent PVCs have adverse effects on pregnancy, and the PVC burden might be an important factor associated with adverse fetal and neonatal outcomes among pregnant women with PVCs. Our cohort study indicated that the higher the PVC burden is, the higher the likelihood of adverse events would be.
Microstructure refinement - homogenization and flexural strength improvement of Al2O3 ceramics fabricated by DLP-stereolithography integrated with chemical precipitation coating process
Authors: Nie Guanglin , Li Yehua , Sheng Pengfei , Zuo Fei , Wu Haolin , Liu Leiren , Deng Xin , Bao Yiwang , Wu Shanghua
Published: October 2020

In this study, the chemical precipitation coating (CP) process was creatively integrated with DLP-stereolithography based 3D printing for refining and homogenizing the microstructure of 3D printed Al2O3 ceramic. Based on this novel approach, Al2O3 powder was coated with a homogeneous layer of amorphous Y2O3; with the coated Al2O3 powder found to make the microstructure of 3D printed Al2O3 ceramic more uniform and refined, as compared with the conventional mechanical mixing (MM) of Al2O3 and Y2O3 powders. The grain size of Al2O3 in Sample CP is 64.44% and 51.43% lower than those in the monolithic Al2O3 ceramic (AL) and Sample MM, respectively. Sample CP has the highest flexural strength of 455.37±32.17 MPa, which is 14.85% and 25.45% higher than those of Samples MM and AL, respectively; also Sample CP has the highest Weibull modulus of 16.88 among the three kinds of samples. Moreover, the fine grained Sample CP has a close thermal conductivity to the coarse grained Sample MM because of the changes in morphology of Y3Al5O12 phase from semi-connected (Sample MM) to isolated (Sample CP). Finally, specially designed fin-type Al2O3 ceramic heat sinks were successfully fabricated via the novel integrated process, which has been proven to be an effective method for fabricating complex-shaped Al2O3 ceramic components with enhanced flexural strength and reliability.
Risk Factors for Mortality of Patients with COVID-19 in Korea: A Nationwide Population-Based Study
Authors: Park Se Jun , Lee Hyungkook , Youk Tae Mi , Jeon Han Ho
Published: October 2020

BackgroundCoronavirus Disease 2019 (COVID-19) is an ongoing major global health crisis caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The outcomes of COVID-19 in patients remain unclear. We aimed to identify risk factors for the mortality of COVID-19 in the Republic of Korea without health system disruption.MethodsThe data of confirmed COVID-19 patients were retrieved from Health Insurance Review and Assessment Service (HIRA) dataset between January 3 and June 1, 2020. We used multivariable logistic regression models to identify risk factors for mortality in patients with COVID-19.ResultsWe identified 7,333 COVID-19 patients, of whom 227 (3.1%) died. Hypertension was the most common (26%), followed by diabetes mellitus (23.0%). In multivariate analysis, male (OR: 1.92, P <0.0001), elderly (age >65) (OR: 11.00, P < 0.0001), living in a large outbreak area (OR: 2.61, P < 0.0001), Medical Aid beneficiaries (OR: 1.54, P = 0.0282), Charlson Comorbidity Index (CCI) score ≥3 (OR: 3.25, P = 0.029), hypertension (OR: 1.90, P = 0.0013), chronic obstructive pulmonary disease (COPD) (OR: 1.61, P = 0.0374), congestive heart failure (CHF) (OR: 2.14, P = 0.0001), and end-stage renal disease (ESRD) (OR: 5.69, P = 0.0090) were independent risk factors associated with mortality in COVID-19 patients. However, diabetes mellitus, liver cirrhosis, and inflammatory bowel disease were not risk factors for mortality. ConclusionMale, elderly patients ˃65 years old, Medical Aid beneficiaries, higher CCI (≥3), and living in a large outbreak area might have a greater risk of mortality and comorbidities such as hypertension, COPD, CHF, and ESRD could also greatly affect the mortality in COVID-19 patients.
Effects of repetitive magnetic stimulation on motor function and expression of GAP-43 and 5-HT in rats with spinal cord injury
Authors: Liu Hao , Xiong Deqi , Wang Wenchun , Yuan Li , Pang Rizhao , Deng Qian , Sun Nianyi , Zheng Jinqi , Liu Jiancheng , Xiang Wu , Chen Zhesi , Lu Jiachun , Zhang Anren
Published: October 2020

Objectives: Spinal cord injury (SCI) is a disastrous central nervous system (CNS) disorder. The aim of this study was to explore the effects of repetitive trans-spinal magnetic stimulation (rTSMS) act on different segments of the spinal cord on movement function and expression of GAP43 and 5-HT in rats after acute cord injury and to preliminarily discuss the best treatment site of rTSMS, so as to provide theoretical foundation and experimental evidence for the clinical application of rTSMS in spinal cord injury. Methods: In the present study, we used a rat model of T10 laminectomy with transient violent oppression by aneurysm clip. The rats were classified into A group (sham surgery), B group (acute SCI without stimulation), C group (T6 segment stimulation), D group (T10 segment stimulation) and E group (L2 segment stimulation). Results: In vivo the magnetic stimulation was found to protect motor function and alleviate myelin sheath damage, decrease the expression levels of NgR and Nogo-A, increase the expression levels of growth-associated protein-43 (GAP43) and 5-hydroxytryptamine (5-HT), and inhibite TUNEL-positive cells as well as the expressions of apoptosis-related protein of rats following 8 weeks post-operation.Conclusions: This study suggests that rTSMS can promote the expression of GAP-43 and 5-HT and axonal regeneration in the spinal cord which is beneficial to the recovery of motor function after acute spinal cord injury.