bluuebunny/crossref_metadata_2025 huggingface dataset:
Vulnerability through the Eyes of People Attended by a Portuguese Community-Based Association: A Thematic Analysis
Authors: Laranjeira Carlos , Piaça Inês , Vinagre Henrique , Vaz Ana Rita , Ferreira Sofia , Cordeiro Lisete , Querido Ana
Published: September 2022

Vulnerability is associated with the individual’s social and biological conditions, but also the conditions of their enveloping environment and society, leading to terms such as vulnerable populations or risk groups. This study aimed to give a voice to people with experiences of vulnerability and explore their perspectives, using a descriptive qualitative design. Purportedly vulnerable adults were recruited and interviewed with semi-structured questions on vulnerability. Data were organized, using WebQDA software, and submitted to thematic content analysis, as proposed by Clark and Braun, which generated a thematic tree. The study included six men and six women with a mean age of 43.8 [SD = 14.17] years old. Thematic analysis generated three themes: (1) Conceptions about vulnerability, (2) Barriers imposed by vulnerability, and (3) Strategies for dealing with vulnerability. The results highlight that vulnerability is a highly dynamic process of openness to circumstances that influence individual outcomes. However, there is a lack of conceptual clarity. Although being vulnerable is perceived as something negative, we need to transform the social mindset, because vulnerability also has the potential to change priorities in life for the better.
Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family
Authors: Kozar Elena V. , Kozar Elena G. , Domblides Elena A.
Published: September 2022

Isolated microspore culture in vitro (IMC) is an advanced technique for producing doubled haploids. We developed a modified microspore isolation method for the Brassicaceae family, which exceeds the results obtained by a standard microspore isolation method. We found that the new method allows an increase in the percentage of microspores at the embryogenic stage of development in the culture. In the spring rapeseed ‘Ratnik’ culture the percentage of microspores increases from 66.7% to 73%, and in the European radish ‘RBK’ from 34% to 61.9%. Moreover, the new method of microspore isolation made it possible to expand the range of linear bud sizes (from 3.5–4.0 to 3.0–4.5 mm for spring rapeseed ‘Ratnik’) suitable for IMC technology. In addition, the new method of microspore isolation reduced the debris in the preparation of spring rapeseed ‘Ratnik’ and European radish ‘RBK’ by 2.4 and 15 times, respectively. The best results were shown on Sareptian mustard No. 72, where the yield of embryoids increased by 7.5 times. Remarkably, the new method of microspore isolation allowed us to obtain the first embryoids of red cabbage No. 428, whereas no embryoids were obtained using the standard method of microspore isolation. In summary, the new method of microspore isolation allows an increase in the efficiency of IMC technology for Brassicaceae family crops.
Cadaveric and Ultrasound Validation of Percutaneous Electrolysis Approach at the Achilles Tendon as a Potential Treatment for Achilles Tendinopathy: A Pilot Study
Authors: Calderón-Díez Laura , Sánchez-Sánchez José Luis , Robles-García Miguel , Belón-Pérez Pedro , Fernández-de-las-Peñas César
Published: September 2022

Achilles tendon tendinopathy (AT) is a musculoskeletal condition characterized by pain in the Achilles tendon and impaired physical performance or sport activities. AT is difficult to treat, and the results are variable. Preliminary evidence suggests a positive effect for pain of percutaneous electrolysis in patients with tendinopathy. Our aim was to determine the validity and safety of a percutaneous electrolysis approach targeting the interphase between the Achilles tendon and the Kager’s fat with ultrasound imaging in both healthy individuals and on a fresh cadaver model (not ultrasound guiding). A needle was inserted from the medial to the lateral side under the body of the Achilles tendon, just between the tendon and the Kager’s triangle, about 5 cm from the insertion of tendon in the calcaneus in 10 healthy volunteers (ultrasound study) and 10 fresh cadaver legs. An accurate needle penetration of the interphase was observed in 100% of the approaches, in both human and cadaveric models. No neurovascular bundle of the sural nerve was pierced in any insertion. The distance from the tip of the needle to the sural nerve was 5.28 ± 0.7 mms in the cadavers and 4.95 ± 0.68 mms in the volunteer subjects, measured in both cases at a distance of 5 cm from the insertion of the Achilles tendon. The results of the current study support that percutaneous electrolysis can be safely performed at the Kager’s fat–Achilles tendon interphase if it is US guided. In fact, penetration of the sural nerve was not observed in any needle approach when percutaneous needling electrolysis was performed by an experienced clinician. Future studies investigating the clinical effectiveness of the proposed intervention are needed.
Artificial Intelligence in Public Health: Current Trends and Future Possibilities
Authors: Giansanti Daniele
Published: September 2022

Artificial intelligence (AI) is a discipline that studies whether and how intelligent computer systems that can simulate the capacity and behaviour of human thought can be created [...]
Exploring the Influence of a Community-Based Peer-Led Wheelchair Skills Training on Satisfaction with Participation in Children and Adolescents with Cerebral Palsy and Spina Bifida: A Pilot Study
Authors: Ouellet Béatrice , Best Krista L. , Wilson Deb , Miller William C.
Published: September 2022

Background: Peer-led approaches improve satisfaction with participation, wheelchair skills and wheelchair use self-efficacy in adults, but the evidence is limited in children. This pilot study aimed to explore the influence of community-based, peer-led, group wheelchair training program (i.e., Seating To Go) on satisfaction with participation (primary outcome), wheelchair skills, and wheelchair use self-efficacy in children and adolescents with cerebral palsy and spina bifida. Methods: A single group pre-post design was used. Invitations were shared online and diffused by clinicians and advocacy and provider groups to recruit a convenience sample of eight pediatric wheelchair users. Participants completed the Seating To Go program in groups that were facilitated by adult wheelchair users. Satisfaction with participation (Wheelchair Outcome Measure-Young People), wheelchair skills (Wheelchair Skills Test), wheelchair use self-efficacy (Wheelchair Use Confidence Scale), and perceived wheelchair skills capacity (Wheelchair Skills Test Questionnaire; proxy rating: parents) were evaluated before and after the Seating To Go program. Descriptive statistics and nonparametric longitudinal data analysis were conducted to explore changes in all outcomes from baseline to post-intervention. Results: Pediatric wheelchair users (ranging in age from 5 to 15 years) and their parents reported statistically significant improvements in satisfaction with participation. The improvements in wheelchair skills and wheelchair confidence were also statistically significant, but not the parents’ perception of their children’s wheelchair skills. Conclusions: A community-based peer-led approach to wheelchair skills training seems promising for improving wheelchair outcomes in pediatric wheelchair users. Further controlled studies with larger samples are warranted.
Urban Heat Island Mitigation: GIS-Based Analysis for a Tropical City Singapore
Authors: Teo Ya Hui , Makani Mohamed Akbar Bin Humayun , Wang Weimeng , Liu Linglan , Yap Jun Hong , Cheong Kang Hao
Published: September 2022

To reduce the pace of climate change and achieve the goals set in Paris Agreement by 2030, Association of Southeast Asian Nations (ASEAN) countries have started to prioritize sustainability as one of their top agendas. Numerous studies have demonstrated that one of the most important issues that must be addressed to halt climate change is the urban heat island (UHI). Given the different mitigation strategies available, the focus of our study here is to assess the influence of green spaces and Green Mark commercial buildings on Singapore’s temperature distribution using non-exhaustive factors related to energy consumption and efficiency. Additionally, this paper examines the effectiveness of green spaces and commercial buildings in reducing the rate of temperature change. This study uses ArcGIS software to map data, perform spatial analysis through cloud-based mapping, and produce visual representations with geographic information systems (GIS) to promote greater insight on the formulation of goals and policy making for strategic management. In comparison to non-commercial districts, our findings show that commercial districts have the lowest percentage of temperature change, an estimated 1.6 percent, due to a high concentration of green spaces and Green Mark commercial buildings. Our research also helps to close the research gaps in determining the efficacy of Green Mark commercial buildings, skyrise greeneries, gardens, and national parks. It also helps to minimize the bottleneck of expensive building costs and environmental damage that would have occurred from a design flaw found too late in the urban planning and construction process.
Disparities in Online Use Behaviours and Chinese Digital Inclusion: A 10-Year Comparison
Authors: Yu Xiao , Liu Shu
Published: September 2022

This study focuses on the disparities in Chinese online use behaviours (frequency and diversity) based on educational background and socioeconomic status over 10 years to reveal the Chinese digital inclusion process. We used the China Family Panel Studies (CFPS) 2010 and 2018 panels and considered the time and cohort effects separately. Ordinary least squares analysis revealed usage frequency. The generalized partial proportional odds model demonstrated participants’ prioritization of online study, work, entertainment, and social activity. The results show that the profile of the individuals with the most time spent online has changed from those with high education and income levels to mid-range education and income levels. Individuals with high education and income levels prefer to use the internet for studying and working. There are no clear preference differences between entertainment and social activities amongst most educational backgrounds and socioeconomic statuses. Regarding frequency of internet use, digital inclusion has spread downwards from the upper to the middle classes. Regarding diverse internet uses, upper-class individuals prefer to conduct capital-enhancing activities, and youth remain the main force for diverse online activities; however, over time, middle-aged groups have increased their capital-enhancing activities, and older adults have increased their digital social activities.
Combined Inhibitory Effect of Canada Goldenrod Invasion and Soil Microplastics on Rice Growth
Authors: Zhao Xiaoxun , Xie Hongliang , Zhao Xin , Zhang Jiaqi , Li Zhiliang , Yin Weiqing , Yuan Aiguo , Zhou Huan , Manan Sehrish , Nazar Mudasir , Iqbal Babar , Li Guanlin , Du Daolin
Published: September 2022

Alien plant invasion and residual soil microplastics (MPs) are growing threats to agricultural crop production. This study determined the adverse effects of Canadian goldenrod (Solidago canadensis L.) invasion and residual soil MPs on rice growth and development. The biomass, phenological indices, photosynthetic parameters, and antioxidant enzyme activities of rice were measured on the 50th and 80th day of post-plantation. Biomass and phenotypic results indicated the more harmful effects of the combination of S. canadensis invasion and residual soil MPs compared to S. canadensis invasion or residual soil MPs effects alone. Moreover, the interaction effect of S. canadensis invasion and residual soil MPs markedly reduced the ascorbate peroxidase and catalase belowground, while they increased in the aboveground parts of the rice. However, the S. canadensis invasion and residual soil MPs interactive treatments lowered the superoxide dismutase concentrations in the belowground parts of the rice plants while elevating the peroxidase and reactive oxygen species concentrations in both the belowground and aboveground parts compared to the other treatments. Among all treatments, S. canadensis invasion alone had the most negligible negative impact on rice biomass and growth indices. Our study suggests that soil MPs could negatively affect crop production with invasive alien plants, and the combined effects were more harmful than either of the single factors. Our findings will lay the groundwork for analyzing the impacts of invasive alien plants on rice crops.
The Bright Side of Skin Autofluorescence Determination in Children and Adolescents with Newly Diagnosed Type 1 Diabetes Mellitus: A Potential Predictor of Remission?
Authors: Podolakova Kristina , Barak Lubomir , Jancova Emilia , Stanik Juraj , Sebekova Katarina , Podracka Ludmila
Published: September 2022

Skin autofluorescence (SAF) is a noninvasive method reflecting tissue accumulation of advanced glycation end products (AGEs). We investigated whether, in newly diagnosed children and adolescents with type 1 diabetes (T1D), this surrogate marker of long-term glycemia is associated with markers of the early manifestation phase, residual secretion capacity of the ß-cells, and the occurrence of remission. SAF was measured in 114 children and adolescents (age: 8.0 ± 4.5 years, 44% girls) at the time of T1D diagnosis, and related to HbA1c, C-peptide, diabetic ketoacidosis, and remission. 56 patients were followed up for 1 year. Seventy-four sex- and age-matched healthy individuals served as controls. SAF was higher in the T1D group compared with controls (1.0 ± 0.2 vs. 0.9 ± 0.2, p < 0.001). At the time of diagnosis, SAF correlated with HbA1c (r = 0.285, p = 0.002), was similar in patients with and without ketoacidosis, and was lower in the remitters compared with non-remitters (0.95 ± 0.18 vs. 1.04 ± 0.26, p = 0.027). Unlike HbA1c, SAF was an independent predictor of remission (∆R2 = 0.051, p = 0.004). Former studies consider SAF in diabetic patients as a tool to identify individuals at an increased risk of chronic complications. Here we show that determination of SAF at the time of T1D diagnosis might potentially predict remission, at least in children.
Can Blood Flow Restriction Training Benefit Post-Activation Potentiation? A Systematic Review of Controlled Trials
Authors: Tian Haodong , Li Hansen , Liu Haowei , Huang Li , Wang Zhenhuan , Feng Siyuan , Peng Li
Published: September 2022

(1) Background: post-activation potentiation (PAP) plays an essential role in enhancing athletic performance. Various conditioning activities (CAs) have been developed to generate PAP before training or competitions. However, whether extra equipment can enhance the effectiveness of CAs is understudied. Hence, this systematic review aims to introduce and examine the effectiveness of blood flow restriction-based conditioning activities (BFR-CAs). (2) Methods: a literature search was conducted via Web of Science, PubMed, SPORTDiscus, and CNKI (a Chinese academic database). The systematic review included the literature concerning BFR-CAs and non-BFR-CAs. The methodological quality of included studies was considered to be “moderate quality” and “good quality” based on the Physiotherapy Evidence Database Scale. (3) Results: five studies were included in this study. Four studies were on lower limb strength training, and three of them suggested a greater PAP in BFR-CAs than in non-BFR counterparts. One study on upper limb strength training also supported the advantage of BFR-CAs. (4) Conclusions: BFR-CAs may be an emerging and promising strategy to generate PAP. Compared with non-BFR-CAs, BFR-CAs might be more efficient and practical for inexperienced sports people or athletes in non-power sports.
Influence of Nature Reserves on the Energy Consumption Structure of Local Farmers
Authors: Chen Ke , Li Runze , Wang Yang
Published: September 2022

The energy consumption of farmers residing around nature reserves is an important factor that affects the coordinated development of nature reserves and the surrounding communities. The optimization of the energy consumption structure of farmers around nature reserves is important for maintaining the resources and environment of nature reserves and saving natural resources. Based on the microscopic survey data for the energy consumption structure of 956 rural households around six nature reserves in Liaoning province, a multiple linear regression model was used in this study to match tendency scores and empirically examine the impacts of regulatory policies of nature reserves on the energy consumption of rural households in Liaoning province. In addition, the influence of the income and environmental cognition of farmers on the energy consumption of rural households around nature reserves was examined. The results showed that the regulatory policies of the nature reserves were conducive to reducing the traditional biomass energy consumption of the farmers. The nature reserves indirectly affect the energy consumption of the farmers by influencing their income, and cognition plays an important role in reducing the traditional biomass energy consumption of farmers in nature reserves. Compared with provincial nature reserves, national nature reserves have a more evident impact on the energy consumption of farmers. Based on the empirical results, farmers should be encouraged to reduce their high-pollution and high-emission energy consumption activities and should be provided with technical support and financial subsidies for clean energy, such as solar energy and biogas. The following measures should be taken to maintain the ecological environment of the nature reserves and to reduce the contradiction between the nature reserves and farmers: supervise the coal quality in the surrounding areas of nature reserves, improve the non-agricultural employment ability of farmers around nature reserves and the photovoltaic poverty alleviation project in Liaoning province, increase the income of farmers and promote the energy consumption of farmers around nature reserves, strengthen the management of provincial nature reserves, promptly change the traditional idea of ‘depend on the mountain and water’ adopted by farmers, improve the environmental awareness of farmers residing around nature reserves, and advocate green energy consumption.
Anxiety, Depression, and Post-Traumatic Stress Disorder (PTSD) Symptomatology According to Gender in Health-Care Workers during the COVID-19 Pandemic in Peru Shortened Title: “Psychological Impact of the Pandemic on Women”
Authors: Martínez Pajuelo Alex Ricardo , Irrazabal Ramos José Eduardo , Lazo-Porras Maria
Published: September 2022

Objective: The current study will evaluate the association that the COVID-19 pandemic has had with health-care workers and identify the factors that influenced the female gender being more affected. Methods: This is a cross-sectional study conducted in two hospitals in Arequipa (a Peruvian city). The participants were health-care workers. We applied a questionnaire with sociodemographic information and three scales: the Generalized Anxiety Disorder-7, the Patient Health Questionnaire-9, and the Primary Care Post-Traumatic Stress Disorder (PTSD) Screen for DSM-5. The main outcomes were anxiety, depression, and PTSD scores. The exposure of interest was gender. The scores of the scales were estimated by medians and percentiles 25–75 (p25–p75), and we used linear regression to estimate the crude and adjusted coefficients and their respective confidence intervals at 95% (CI 95%). Results: There were 109 participants, and 43.1% were women. The anxiety, depression, and PTSD median (p25–p75) scores in the study population were 6 (2–11), 6 (2–10), and 1 (0–3), respectively. The adjusted analysis showed that the female sex had 4.48 (CI 95% 2.95–6.00), 4.50 (CI 95% 2.39–6.62), and 1.13 (CI 95% 0.50–1.76) higher points on average for the scales of anxiety, depression, and PTSD symptoms in comparison to males, respectively. Conclusions: Female health-care workers showed increased scores of mental health issues in comparison to male health-care workers.
Healthcare Workers’ Emotions, Perceived Stressors, and Coping Strategies at Workplace during the COVID-19 Pandemic in Palestine
Authors: Ahmead Muna , El Sharif Nuha , Asad Samer
Published: September 2022

Evidence about the impact of the COVID-19 pandemic on the mental health of Palestinian healthcare professionals is lacking and has been disregarded during the COVID-19 pandemic. This study aims to describe Palestinian healthcare workers (HCWs) emotions and factors causing stress, and factors used to reduce the stress experienced at the workplace and to examine the relationships between HCWs level of stress and their coping strategies and motivational factors during the COVID-19 pandemic. A self-reported online survey was completed by 506 doctors and nurses working in healthcare facilities that provide COVID-19 care. Descriptive statistics, bivariate, and multivariate regression models were developed to adjust for the association between HCWs coping and motivation factors with HCWs’ perceived stressors. The findings showed that 24.1% of the participants had a high-stress level, and 25.7% had a low level of stress. The participant’s main coping strategy was avoidance and the key emotional factor was the ethical and professional obligation to continue working. Additionally, a major cause of their stress was their personal safety and having the COVID-19 infection. Findings showed a positive association between stress and the younger age of participants, with physicians being less stressed than nurses. In addition, receiving no training on the treatment of COVID-19 was strongly associated with stress levels. Furthermore, there was a significant positive correlation between stress scoring and coping strategies scoring but not with motivation score. In conclusion, this study shows that Palestinian healthcare workers experienced emotional turmoil during the COVID-19 pandemic. These results indicate the necessity of providing supervision, psychological counseling and intervention to professional healthcare workers who work directly with COVID-19 patients in health settings during the current pandemic or in the event of future outbreaks. Policymakers and managers must also conduct training and provide interventions on how to cope with stress in pandemics, in order to assist HCWs in developing their adaptive coping strategies and increase their motivation
Is There a Spatial Relationship between Urban Landscape Pattern and Habitat Quality? Implication for Landscape Planning of the Yellow River Basin
Authors: Zhang Dike , Wang Jianpeng , Wang Ying , Xu Lei , Zheng Liang , Zhang Bowen , Bi Yuzhe , Yang Hui
Published: September 2022

The extent to which landscape spatial patterns can impact the dynamics and distribution of biodiversity is a key geography and ecology issue. However, few previous studies have quantitatively analyzed the spatial relationship between the landscape pattern and habitat quality from a simulation perspective. In this study, the landscape pattern in 2031 was simulated using a patch-generating simulation (PLUS) model for the Yellow River Basin. Then, the landscape pattern index and habitat quality from 2005 to 2031 were evaluated using the Fragstats 4.2 and the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model. Furthermore, we analyzed the spatial distribution characteristics and spatial spillover effects of habitat quality using spatial autocorrelation analysis. Finally, the spatial association between the landscape pattern index and habitat quality was quantitatively revealed based on a spatial lag model. The simulation results showed that: (1) from 2005 to 2031, the landscape of the Yellow River Basin would be dominated by grassland and unused land, and the areas of construction land and water body will increase significantly, while the area of grassland will decrease; (2) patch density (PD) and Shannon’s diversity index (SHDI) show significant increases, while edge density (ED), landscape shape index (LSI), mean patch area (AREA_MN), and contagion index (CONTAG) decrease; (3) from 2005 to 2031, habitat quality would decrease. The high-value areas of habitat quality are mainly distributed in the upper reaches of the Yellow River Basin, and the low-value areas are distributed in the lower reaches. Meanwhile, both habitat quality and its change rate present positive spatial autocorrelation; and (4) the spatial relationships of habitat quality with PD and COHESION are negative, while ED and LSI have positive impacts on habitat quality. Specifically, landscape fragmentation caused by high PD has a dominant negative influence on habitat quality. Therefore, this study can help decision makers manage future landscape patterns and develop ecological conservation policy in the Yellow River Basin.
LSSmScarlet2 and LSSmScarlet3, Chemically Stable Genetically Encoded Red Fluorescent Proteins with a Large Stokes’ Shift
Authors: Subach Oksana M. , Vlaskina Anna V. , Agapova Yulia K. , Piatkevich Kiryl D. , Patrushev Maxim V. , Samygina Valeriya R. , Subach Fedor V.
Published: September 2022

Red fluorescent proteins with a large Stokes’ shift (LSSRFPs) are genetically encoded and efficiently excited by 488 nm light, allowing simultaneous dual-color one- and two-photon fluorescence imaging and fluorescence correlation spectroscopy in combination with green fluorescent proteins FPs. Recently, based on the conventional bright mScarlet RFP, we developed the LSSRFP LSSmScarlet. LSSmScarlet is characterized by two pKa values at pH values of 1.9 and 5.8. In this study, we developed improved versions of LSSmScarlet, named LSSmScarlet2 and LSSmScarlet3, which are characterized by a Stokes’ shift of 128 nm and extreme pH stability with a single pKa value of 2.2. LSSmScarlet2 and LSSmScarlet3 had 1.8-fold faster and 3-fold slower maturation than LSSmScarlet, respectively. In addition, both LSSRFPs were 1.5- to 1.6-fold more photostable and more chemically resistant to denaturation by guanidinium chloride and guanidinium thiocyanate. We also compared the susceptibility of the LSSmScarlet2, LSSmScarlet3, and other LSSRFPs to the reagents used for whole-mount imaging, expansion microscopy, and immunostaining techniques. Due to higher pH stability and faster maturation, the LSSmScarlet3-LAMP3 fusion was 2.2-fold brighter than LSSmScarlet-LAMP3 in lysosomes of mammalian cells. The LSSmScarlet3-hLAMP2A fusion was similar in brightness to LSSmScarlet-hLAMP2A in lysosomes. We successfully applied the monomeric LSSmScarlet2 and LSSmScarlet3 proteins for confocal imaging of structural proteins in live mammalian cells. We also solved the X-ray structure of the LSSmScarlet2 protein at a resolution of 1.41 Å. Site-directed mutagenesis of the LSSmScarlet2 protein demonstrated the key role of the T74 residue in improving the pH and chemical stability of the LSSmScarlet2 protein.
UvSorA and UvSorB Involved in Sorbicillinoid Biosynthesis Contribute to Fungal Development, Stress Response and Phytotoxicity in Ustilaginoidea virens
Authors: Zhang Xuping , Xu Dan , Hou Xuwen , Wei Penglin , Fu Jiajin , Zhao Zhitong , Jing Mingpeng , Lai Daowan , Yin Wenbing , Zhou Ligang
Published: September 2022

Ustilaginoidea virens (teleomorph: Villosiclava virens) is an important fungal pathogen that causes a devastating rice disease. It can produce mycotoxins including sorbicillinoids. The biosynthesis and biological functions of sorbicillinoids have not been reported in U. virens. In this study, we identified a sorbicillinoid biosynthetic gene cluster in which two polyketide synthase genes UvSorA and UvSorB were responsible for sorbicillinoid biosynthesis in U. virens. In ∆UvSorA and ∆UvSorB mutants, the mycelial growth, sporulation and hyphal hydrophobicity were increased dramatically, while the resistances to osmotic pressure, metal cations, and fungicides were reduced. Both phytotoxic activity of rice germinated seeds and cell wall integrity were also reduced. Furthermore, mycelia and cell walls of ∆UvSorA and ∆UvSorB mutants showed alterations of microscopic and submicroscopic structures. In addition, feeding experiment showed that sorbicillinoids could restore mycelial growth, sporulation, and cell wall integrity in ∆UvSorA and ∆UvSorB mutants. The results demonstrated that both UvSorA and UvSorB were responsible for sorbicillinoid biosynthesis in U. virens, and contributed to development (mycelial growth, sporulation, and cell wall integrity), stress responses, and phytotoxicity through sorbicillinoid mediation. It provides an insight into further investigation of biological functions and biosynthesis of sorbicillinoids.
Analysis of Tumor-Infiltrating T-Cell Transcriptomes Reveal a Unique Genetic Signature across Different Types of Cancer
Authors: Vidal Mabel , Fraga Marco , Llerena Faryd , Vera Agustín , Hernández Mauricio , Koch Elard , Reyes-López Felipe , Vallejos-Vidal Eva , Cabrera-Vives Guillermo , Nova-Lamperti Estefanía
Published: September 2022

CD8+ and CD4+ T-cells play a key role in cellular immune responses against cancer by cytotoxic responses and effector lineages differentiation, respectively. These subsets have been found in different types of cancer; however, it is unclear whether tumor-infiltrating T-cell subsets exhibit similar transcriptome profiling across different types of cancer in comparison with healthy tissue-resident T-cells. Thus, we analyzed the single cell transcriptome of five tumor-infiltrating CD4-T, CD8-T and Treg cells obtained from different types of cancer to identify specific pathways for each subset in malignant environments. An in silico analysis was performed from single-cell RNA-sequencing data available in public repositories (Gene Expression Omnibus) including breast cancer, melanoma, colorectal cancer, lung cancer and head and neck cancer. After dimensionality reduction, clustering and selection of the different subpopulations from malignant and nonmalignant datasets, common genes across different types of cancer were identified and compared to nonmalignant genes for each T-cell subset to identify specific pathways. Exclusive pathways in CD4+ cells, CD8+ cells and Tregs, and common pathways for the tumor-infiltrating T-cell subsets were identified. Finally, the identified pathways were compared with RNAseq and proteomic data obtained from T-cell subsets cultured under malignant environments and we observed that cytokine signaling, especially Th2-type cytokine, was the top overrepresented pathway in Tregs from malignant samples.
Clinical Trials of New Drugs for Vascular Cognitive Impairment and Vascular Dementia
Authors: Linh Tran Thanh Duy , Hsieh Yi-Chen , Huang Li-Kai , Hu Chaur-Jong
Published: September 2022

Population aging has challenged the treatment of cognitive impairment or dementia. Vascular dementia is the second leading cause of dementia after Alzheimer’s disease. Cognitive consequences after ischemic brain injury have been recognized as a preferred target for therapeutic strategies, prompting the search for potential agents. The keyword “vascular dementia” was used to search ClinicalTrials.gov to determine agents represented in phases I, II, III, and IV. The agents were classified on the basis of their mechanisms. Of the 17 randomized controlled trials meeting our inclusion criteria, 9 were completed in the past 10 years, and 8 are ongoing or in the planning stages. We also identified one trial in phase I, nine in phase II, six in phase III, and one in phase IV. Fewer trials of new drugs for improving cognition or ameliorating the behavioral and psychological symptoms of dementia target vascular dementia than Alzheimer’s dementia. Drug trials on vascular dementia overlap with drug trials targeting functional outcomes in cerebrovascular disease. International pharmaceutical companies’ investment in new drugs targeting VCI and vascular dementia remains insufficient.
Automatic Segmentation of Laser-Induced Injury OCT Images Based on a Deep Neural Network Model
Authors: Gao Tianxin , Liu Shuai , Gao Enze , Wang Ancong , Tang Xiaoying , Fan Yingwei
Published: September 2022

Optical coherence tomography (OCT) has considerable application potential in noninvasive diagnosis and disease monitoring. Skin diseases, such as basal cell carcinoma (BCC), are destructive; hence, quantitative segmentation of the skin is very important for early diagnosis and treatment. Deep neural networks have been widely used in the boundary recognition and segmentation of diseased areas in medical images. Research on OCT skin segmentation and laser-induced skin damage segmentation based on deep neural networks is still in its infancy. Here, a segmentation and quantitative analysis pipeline of laser skin injury and skin stratification based on a deep neural network model is proposed. Based on the stratification of mouse skins, a laser injury model of mouse skins induced by lasers was constructed, and the multilayer structure and injury areas were accurately segmented by using a deep neural network method. First, the intact area of mouse skin and the damaged areas of different laser radiation doses are collected by the OCT system, and then the labels are manually labeled by experienced histologists. A variety of deep neural network models are used to realize the segmentation of skin layers and damaged areas on the skin dataset. In particular, the U-Net model based on a dual attention mechanism is used to realize the segmentation of the laser-damage structure, and the results are compared and analyzed. The segmentation results showed that the Dice coefficient of the mouse dermis layer and injury area reached more than 0.90, and the Dice coefficient of the fat layer and muscle layer reached more than 0.80. In the evaluation results, the average surface distance (ASSD) and Hausdorff distance (HD) indicated that the segmentation results are excellent, with a high overlap rate with the manually labeled area and a short edge distance. The results of this study have important application value for the quantitative analysis of laser-induced skin injury and the exploration of laser biological effects and have potential application value for the early noninvasive detection of diseases and the monitoring of postoperative recovery in the future.
Mast Cells and Dendritic Cells as Cellular Immune Checkpoints in Immunotherapy of Solid Tumors
Authors: Kalkusova Katerina , Smite Sindija , Darras Elea , Taborska Pavla , Stakheev Dmitry , Vannucci Luca , Bartunkova Jirina , Smrz Daniel
Published: September 2022

The immune checkpoint inhibitors have revolutionized cancer immunotherapy. These inhibitors are game changers in many cancers and for many patients, sometimes show unprecedented therapeutic efficacy. However, their therapeutic efficacy is largely limited in many solid tumors where the tumor-controlled immune microenvironment prevents the immune system from efficiently reaching, recognizing, and eliminating cancer cells. The tumor immune microenvironment is largely orchestrated by immune cells through which tumors gain resistance against the immune system. Among these cells are mast cells and dendritic cells. Both cell types possess enormous capabilities to shape the immune microenvironment. These capabilities stage these cells as cellular checkpoints in the immune microenvironment. Regaining control over these cells in the tumor microenvironment can open new avenues for breaking the resistance of solid tumors to immunotherapy. In this review, we will discuss mast cells and dendritic cells in the context of solid tumors and how these immune cells can, alone or in cooperation, modulate the solid tumor resistance to the immune system. We will also discuss how this modulation could be used in novel immunotherapeutic modalities to weaken the solid tumor resistance to the immune system. This weakening could then help other immunotherapeutic modalities engage against these tumors more efficiently.
Dual Effect of Tryptamine on Prostate Cancer Cell Growth Regulation: A Pilot Study
Authors: Li Zhuangzhuang , Ding Baoyan , Ali Mustafa R. K. , Zhao Lizhen , Zang Xiaoling , Lv Zhihua
Published: September 2022

Abnormal tryptophan metabolism is linked to cancer and neurodegenerative diseases, and tryptophan metabolites have been reported as potential prostate cancer (PCa) biomarkers. However, little is known about the bioactivities of tryptophan metabolites on PCa cell growth. In this study, MTT and transwell assays were used to study the cytotoxicities of 13 major tryptophan metabolites on PCa and normal prostate epithelial cell lines. Ultraperformance liquid chromatography–high resolution mass spectrometry (UPLC–HRMS) was used to analyze metabolic changes in cells treated with tryptamine. Flow cytometry, confocal imaging, and Western blot were used to test the apoptosis induced by tryptamine. It was shown that tryptamine had obvious inhibitory effects on PCa cell lines PC-3 and LNCaP, stronger than those on the normal prostate cell line RWPE-1. Tryptamine was further shown to induce apoptosis and inhibit PC-3 cell migration. Metabolic changes including amino acid metabolism related to cell proliferation and metastasis were found in PC-3 cells treated with tryptamine. Furthermore, a PC-3 xenograft mouse model was used to study the effect of tryptamine in vivo. The intratumoral injection of tryptamine was demonstrated to significantly reduce the tumor growth and tumor sizes in vivo; however, intraperitoneal treatment resulted in increased tumor growth. Such dual effects in vivo advanced our understanding of the bioactivity of tryptamine in regulating prostate tumor development, in addition to its major role as a neuromodulator.
Effects of Dried Onion Powder and Quercetin on Obesity-Associated Hepatic Menifestation and Retinopathy
Authors: Chang Wen-Lung , Liu Pei-Yi , Yeh Shu-Lan , Lee Huei-Jane
Published: September 2022

Onion (Allium cepa L.), rich in flavonoids (particularly quercetin), reportedly has anti-obesity properties, but the underlying mechanisms and associated health issues remain unclear. In this study, we compared the effects of dried onion powder (DO) with that of quercetin on high-fat diet (HFD)-induced obesity, nonalcoholic fatty liver disease, and retinal neovascularization. Briefly, rats (n = 9–10 per group) were divided into control, HFD alone (43% fat), HFD + DO (1% DO), HFD + 5DO (5% DO, w/w), and HFD + quercetin (180 mg/kg). After 12 weeks, body fat, markers of metabolism, fatty liver, steatohepatitis, and retinopathy were analyzed. The results revealed that DO and 5DO dose-dependently suppressed body weight, visceral and subcutaneous fat accumulation, and epididymal adipocyte in HFD-fed rats. DO also decreased HFD-induced ALT, AST, free fatty acid, glucose, proinflammatory cytokines, and oxidative stress. DO and 5DO groups had lower triglycerides, total cholesterol, proinflammatory cytokine levels, and ACC-α (a fatty acid synthesis–associated enzyme) expression but higher hepatic antioxidant enzyme activities and fecal lipids. 5DO exhibited better or similar efficacy to quercetin. Both 5DO and quercetin increased fecal levels of acetic acid and butyric acid similarly. They also reduced lipid peroxidation of the eye, retinal adiposity, and neovascularization. However, quercetin resulted in a more apparent decrease in regulation of the Raf/MAPK pathway than DO in eye specimens. Conclusively, DO suppresses visceral, subcutaneous, and liver fat accumulation better than quercetin likely due to higher fecal fat excretion and lower oxidative stress, proinflammatory cytokine levels, and ACC-α expression. Quercetin regulating signal pathways is better than DO at reducing retinal adiposity and neovascularization.
SHP-1/STAT3-Signaling-Axis-Regulated Coupling between BECN1 and SLC7A11 Contributes to Sorafenib-Induced Ferroptosis in Hepatocellular Carcinoma
Authors: Huang Chao-Yuan , Chen Li-Ju , Chen Grace , Chao Tzu-I , Wang Cheng-Yi
Published: September 2022

Ferroptosis is a type of iron-dependent cell death pertaining to an excess of lipid peroxidation. It has been suggested that sorafenib—an anti-angiogenic medication for hepatocellular carcinoma (HCC)—induces ferroptosis, but the underlying mechanism for this remains largely unknown. We employed siRNA-mediated gene silencing to investigate the role of Src homology region 2 domain-containing phosphatase-1 (SHP-1), following sorafenib treatment, in cystine/glutamate-antiporter-system-Xc−-regulated cystine uptake. Co-immunoprecipitation was also performed to examine the interactions between MCL1, beclin 1 (BECN1), and solute carrier family 7 member 11 (SLC7A11), which functions as the catalytic subunit of system Xc−. The results of this study showed that sorafenib enhanced the activity of SHP-1, dephosphorylated STAT3, downregulated the expression of MCL1 and, consequently, reduced the association between MCL1 and BECN1. In contrast, increased binding between BECN1 and SLC7A11 was observed following sorafenib treatment. The elevated interaction between BECN1 and SLC7A11 inhibited the activity of system Xc−, whereas BECN1 silencing restored cystine intake and protected cells from ferroptosis. Notably, ectopic expression of MCL1 uncoupled BECN1 from SLC7A11 and rescued cell viability by attenuating lipid peroxidation. The results revealed that ferroptosis could be induced in HCC via SHP-1/STAT3-mediated downregulation of MCL1 and subsequent inhibition of SLC7A11 by increased BECN1 binding.
Formation of Unique Placental Seed Capsules in the Maturation Process of the Tomato Fruit
Authors: Chaban Inna A. , Gulevich Alexander A. , Baranova Ekaterina N.
Published: September 2022

The morphological and anatomical study of the seed formation features in a juicy tomato fruit was carried out. The ovules, which form on the placenta, have been shown to be gradually enveloped by the protrusions of placental tissue that arises simultaneously with them. As a result of this process, each seed is enclosed in an individual capsule. These seed capsules have been shown in vivo to be airtight and air-filled. Tomato seeds, as has been shown in this study, develop inside these capsules until the full maturity of the fruit and do not come into contact with the detached and moldered cells of the placenta protrusions, which convert into a gel (pulp). Using scanning electron microscopy, it was possible to reveal the details of a ribbon-like “pubescence” formation of the tomato seed, as well as to understand the mechanism of cracking of the outer layer cells in the seed coat, associated with the detection of calcium oxalate crystals in these cells. The unique outer layer of the tomato seed coat seems to play the role of a scaffold that maintains a constant volume of the protective capsule.
Identification and Characterization of AUXIN Response Factor Gene Family Reveals Their Regulatory Network to Respond the Multi-Hormones Crosstalk during GA-Induced Grape Parthenocarpic Berry
Authors: Sheng Zilu , Xuan Xuxian , Wang Fei , Sadeghnezhad Ehsan , Gong Peijie , Xiao Yingke , Dong Tianyu , Zhang Peian , Wang Xicheng , Fang Jinggui , Wang Chen
Published: September 2022

Exogenous gibberellin (GA) was widely used to improve berry quality through inducing parthenocarpic seedless berries in grapes. We revealed that auxin response factors (ARFs), the key transcription factors in response to auxin, might respond to GA involving modulation of grape parthenocarpy. However, the underlying molecular mechanism in this process remains yet unclear. Here, a total of 19 VvARF members were identified in the ovaries during GA-induced grapes’ parthenocarpy. Interestingly, almost all members were GA-responsive factors, of which 9 could be classified in plant hormone signal transduction (KO04075) and involved in the tryptophan metabolic pathway (K14486). Moreover, VvARFs were predicted to have 310 interacted proteins involved in 19 KEGG pathways. Of them, 32 interacted proteins participated in the KO04075 pathway, including auxin (IAA), salicylic acid (SA), abscisic acid (ABA), cytokinin (CTK), and ethylene signaling pathways by responding to GA-mediated multi-hormone crosstalk. Further analysis demonstrated that VvARF4-2 might be the major factor in the modulation of GA-induced parthenocarpy via the crosstalk of IAA, CTK, SA, and ethylene signaling, followed by VvARF6-1 and VvARF9 involved in SA and ABA signaling pathways, respectively. Finally, we developed a VvARFs-mediated regulatory network by responding to GA-mediated multi-hormone crosstalk during grape parthenocarpy. Collectively, our findings provided novel insights into the regulatory network of VvARFs in GA-guided multi-hormone signaling to modulate grape parthenocarpy, which has great implications for the molecular breeding of high quality seedless grape berries.
The Gut–Immune–Brain Axis: An Important Route for Neuropsychiatric Morbidity in Inflammatory Bowel Disease
Authors: Masanetz Rebecca Katharina , Winkler Jürgen , Winner Beate , Günther Claudia , Süß Patrick
Published: September 2022

Inflammatory bowel disease (IBD) comprises Crohn’s disease (CD) and ulcerative colitis (UC) and is associated with neuropsychiatric symptoms like anxiety and depression. Both conditions strongly worsen IBD disease burden. In the present review, we summarize the current understanding of the pathogenesis of depression and anxiety in IBD. We present a stepwise cascade along a gut–immune–brain axis initiated by evasion of chronic intestinal inflammation to pass the epithelial and vascular barrier in the gut and cause systemic inflammation. We then summarize different anatomical transmission routes of gut-derived peripheral inflammation into the central nervous system (CNS) and highlight the current knowledge on neuroinflammatory changes in the CNS of preclinical IBD mouse models with a focus on microglia, the brain-resident macrophages. Subsequently, we discuss how neuroinflammation in IBD can alter neuronal circuitry to trigger symptoms like depression and anxiety. Finally, the role of intestinal microbiota in the gut–immune–brain axis in IBD will be reviewed. A more comprehensive understanding of the interaction between the gastrointestinal tract, the immune system and the CNS accounting for the similarities and differences between UC and CD will pave the path for improved prediction and treatment of neuropsychiatric comorbidities in IBD and other inflammatory diseases.
CD3+/CD56+ NKT-like Cells Show Imbalanced Control Immediately after Exercise in Delayed-Onset Muscle Soreness
Authors: Sonkodi Balázs , Pállinger Éva , Radovits Tamás , Csulak Emese , Shenker-Horváth Kinga , Kopper Bence , Buzás Edit I. , Sydó Nóra , Merkely Béla
Published: September 2022

The purpose of the study was to carry out an immunophenotypical characterization with a special focus on natural killer cells of junior swimmers from the Hungarian National Swim Team before and after an intensive acute exercise. Nineteen swimmers, ten females and nine males, completed the exercise protocol. Sixteen swimmers experienced delayed-onset muscle soreness. Most of our findings substantiated earlier results, such as the increase in the percentage of the CD3−/CD56+ natural killer cells and the CD3−/CD56dim+ NK cells, and the decrease in the percentage of CD3+ T cells among lymphocytes after the exercise protocol. The drop of natural killer cell activity back to the pre-exercise level was in line with earlier findings. Interestingly, the percentage of CD3+/CD56+ NKT-like cells did not change significantly in those three swimmers who did not report delayed-onset muscle soreness. On the contrary, the percentage of CD3+/CD56+ NKT-like cells among lymphocytes increased in fourteen and decreased in two swimmers reporting delayed-onset muscle soreness. This study for the first time demonstrated a link between the delayed-onset muscle soreness and the imbalanced control of CD3+/CD56+ NKT-like cells among lymphocytes. However, validation of this association in a larger sample size study will be necessary.