bluuebunny/crossref_metadata_2025 huggingface dataset:
Developing Models to Predict the Number of Fire Hotspots from an Accumulated Fuel Dryness Index by Vegetation Type and Region in Mexico
Authors: Vega-Nieva D. , Briseño-Reyes J. , Nava-Miranda M. , Calleros-Flores E. , López-Serrano P. , Corral-Rivas J. , Montiel-Antuna E. , Cruz-López M. , Cuahutle M. , Ressl R. , Alvarado-Celestino E. , González-Cabán A. , Jiménez E. , Álvarez-González J. , Ruiz-González A. , Burgan R. , Preisler H.
Published: April 2018

Understanding the linkage between accumulated fuel dryness and temporal fire occurrence risk is key for improving decision-making in forest fire management, especially under growing conditions of vegetation stress associated with climate change. This study addresses the development of models to predict the number of 10-day observed Moderate-Resolution Imaging Spectroradiometer (MODIS) active fire hotspots—expressed as a Fire Hotspot Density index (FHD)—from an Accumulated Fuel Dryness Index (AcFDI), for 17 main vegetation types and regions in Mexico, for the period 2011–2015. The AcFDI was calculated by applying vegetation-specific thresholds for fire occurrence to a satellite-based fuel dryness index (FDI), which was developed after the structure of the Fire Potential Index (FPI). Linear and non-linear models were tested for the prediction of FHD from FDI and AcFDI. Non-linear quantile regression models gave the best results for predicting FHD using AcFDI, together with auto-regression from previously observed hotspot density values. The predictions of 10-day observed FHD values were reasonably good with R2 values of 0.5 to 0.7 suggesting the potential to be used as an operational tool for predicting the expected number of fire hotspots by vegetation type and region in Mexico. The presented modeling strategy could be replicated for any fire danger index in any region, based on information from MODIS or other remote sensors.
Biomass Carbon Sequestration Potential by Riparian Forest in the Tarim River Watershed, Northwest China: Implication for the Mitigation of Climate Change Impact
Authors: Aishan Tayierjiang , Betz Florian , Halik Ümüt , Cyffka Bernd , Rouzi Aihemaitijiang
Published: April 2018

Carbon management in forests has become the most important agenda of the first half of the 21st century in China in the context of the mitigation of climate change impact. As the main producer of the inland river basin ecosystem in arid region of Northwest China, the desert riparian forest maintains the regional environment and also holds a great significance in regulating the regional/global carbon cycle. In this study, we estimated the total biomass, carbon storage, as well as monetary ecosystem service values of desert riparian Populus euphratica Oliv. in the lower reaches of the Tarim River based on terrestrial forest inventory data within an area of 100 ha (100 plots with sizes of 100 m × 100 m) and digitized tree data within 1000 ha (with 10 m × 10 m grid) using a statistical model of biomass estimation against tree height (TH) and diameter at breast height (DBH) data. Our results show that total estimated biomass and carbon storage of P. euphratica within the investigated area ranged from 3.00 to 4317.00 kg/ha and from 1.82 to 2158.73 kg/ha, respectively. There was a significant negative relationship (p < 0.001) between biomass productivity of these forests and distance to the river and groundwater level. Large proportions of biomass (64% of total biomass) are estimated within 200 m distance to the river where groundwater is relatively favorable for vegetation growth and biomass production. However, our data demonstrated that total biomass showed a sharp decreasing trend with increasing distance to the river; above 800 m distance, less biomass and carbon storage were estimated. The total monetary value of the ecosystem service “carbon storage” provided by P. euphratica was estimated to be $6.8 × 104 USD within the investigated area, while the average monetary value was approximately $70 USD per ha, suggesting that the riparian forest ecosystem in the Tarim River Basin should be considered a relevant regional carbon sink. The findings of this study help to establish a better understanding of the spatial distribution pattern of P. euphratica forest under water scarcity and can also provide an alternative approach to local decision-makers for efficient and precise assessment of forest carbon resources for emission reduction programs.
Elastic and Strength Properties of Heat-Treated Beech and Birch Wood
Authors: Borůvka Vlastimil , Zeidler Aleš , Holeček Tomáš , Dudík Roman
Published: April 2018

This paper deals with the impact of heat treatment on the elastic and strength properties of two diffuse porous hardwoods, namely Fagus sylvatica and Betula pendula. Two degrees of the heat treatment were used at temperatures of 165 °C and 210 °C. The dynamic and static elasticity modulus, bending strength, impact toughness, hardness, and density were tested. It is already known that an increase in treatment temperature decreases the mechanical properties and, on the other hand, leads to a better shape and dimensional stability. Higher temperatures of the heat treatment correlated with lower elastic and strength properties. In the case of higher temperature treatments, the decline of tested properties was noticeable as a result of serious changes in the chemical composition of wood. It was confirmed that at higher temperature stages of treatment, there was a more pronounced decrease in beech properties compared to those of the birch, which was the most evident in their bending strength and hardness. Our research confirmed that there is no reason to consider birch wood to be of a lesser quality, although it is regarded by foresters as an inferior tree species. After the heat treatment, the wood properties are almost the same as in the case of beech wood.
Practical Aspects of X-ray Imaging Polarimetry of Supernova Remnants and Other Extended Sources
Authors: Vink Jacco , Zhou Ping
Published: April 2018

The new generation of X-ray polarisation detectors, the gas pixel detectors, which will be employed by the future space missions IXPE and eXTP, allows for spatially resolved X-ray polarisation studies. This will be of particular interest for X-ray synchrotron emission from extended sources like young supernova remnants and pulsar wind nebulae. Here we report on employing a polarisation statistic that can be used to makes maps in the Stokes I, Q, and U parameters, a method that we expand by correcting for the energy-dependent instrumental modulation factor, using optimal weighting of the signal. In order to explore the types of Stokes maps that can be obtained, we present a Monte Carlo simulation program called xpolim, with which different polarisation weighting schemes are explored. We illustrate its use with simulations of polarisation maps of young supernova remnants, after having described the general science case for polarisation studies of supernova remnants, and its connection to magnetic-field turbulence. We use xpolim simulations to show that in general deep, ~2 Ms observations are needed to recover polarisation signals, in particular for Cas A, for which in the polarisation fraction may be as low as 5%.
Mythological Recuperation and Performance as Agency for Genealogical Return in Djanet Sears’s Afrika Solo
Authors: Balogun Lekan
Published: April 2018

This paper is an examination of Djanet Sears’s Afrika Solo (1990) as a unique example of how Blacks in the global diaspora trace their genealogical roots back to Africa. Drawing from research in anthropology, cultural studies, and performance, the paper purports that Sears’s African-Canadian identity is underlined by her recuperation of a heritage, epistemes and performative aesthetics, and, real or imagined, practices that are not just Afrocentric but specifically Yoruba. Essentially, the paper examines Afrika Solo in the context of Black Aesthetic and more significantly as “text” in a Yoruba sense, which constitutes her own way of “going back to get it.” The paper is divided into two parts: the first part presents a general argument about Sears’s journey back to Africa and the culturally-rooted nature of the performance as opposed to feminist/gender readings of same, while the second part explores ways of understanding the play through the lens of Yoruba ritual and its aesthetics.
Epigenetic Mechanisms Impacting Aging: A Focus on Histone Levels and Telomeres
Authors: Song Shufei , Johnson F.
Published: April 2018

Aging and age-related diseases pose some of the most significant and difficult challenges to modern society as well as to the scientific and medical communities. Biological aging is a complex, and, under normal circumstances, seemingly irreversible collection of processes that involves numerous underlying mechanisms. Among these, chromatin-based processes have emerged as major regulators of cellular and organismal aging. These include DNA methylation, histone modifications, nucleosome positioning, and telomere regulation, including how these are influenced by environmental factors such as diet. Here we focus on two interconnected categories of chromatin-based mechanisms impacting aging: those involving changes in the levels of histones or in the functions of telomeres.
Wnt Signaling in Thyroid Homeostasis and Carcinogenesis
Authors: Ely Kim , Bischoff Lindsay , Weiss Vivian
Published: April 2018

The Wnt pathway is essential for stem cell maintenance, but little is known about its role in thyroid hormone signaling and thyroid stem cell survival and maintenance. In addition, the role of Wnt signaling in thyroid cancer progenitor cells is also unclear. Here, we present emerging evidence for the role of Wnt signaling in somatic thyroid stem cell and thyroid cancer stem cell function. An improved understanding of the role of Wnt signaling in thyroid physiology and carcinogenesis is essential for improving both thyroid disease diagnostics and therapeutics.
Socioeconomic Impact Evaluation for Near Real-Time Flood Detection in the Lower Mekong River Basin
Authors: Oddo Perry , Ahamed Aakash , Bolten John
Published: April 2018

Flood events pose a severe threat to communities in the Lower Mekong River Basin. The combination of population growth, urbanization, and economic development exacerbate the impacts of these events. Flood damage assessments, critical for understanding the effects of flooding on the local population and informing decision-makers about future risks, are frequently used to quantify the economic losses due to storms. Remote sensing systems provide a valuable tool for monitoring flood conditions and assessing their severity more rapidly than traditional post-event evaluations. The frequency and severity of extreme flood events are projected to increase, further highlighting the need for improved flood monitoring and impact analysis. In this study we integrate a socioeconomic damage assessment model with a near real-time flood remote sensing and decision support tool (NASA’s Project Mekong). Direct damages to populations, infrastructure, and land cover are assessed using the 2011 Southeast Asian flood as a case study. Improved land use/land cover and flood depth assessments result in rapid loss estimates throughout the Mekong River Basin. Results suggest that rapid initial estimates of flood impacts can provide valuable information to governments, international agencies, and disaster responders in the wake of extreme flood events.
Effects of Co-Processing Sewage Sludge in the Cement Kiln on PAHs, Heavy Metals Emissions and the Surrounding Environment
Authors: Lv Dong , Zhu Tianle , Liu Runwei , Li Xinghua , Zhao Yuan , Sun Ye , Wang Hongmei , Zhang Fan , Zhao Qinglin
Published: April 2018

To understand the effects of co-processing sewage sludge in the cement kiln on non-criterion pollutants emissions and its surrounding environment, the flue gas from a cement kiln stack, ambient air and soil from the background/downwind sites were collected in the cement plant. Polycyclic aromatic hydrocarbons (PAHs) and heavy metals of the samples were analyzed. The results show that PAHs in flue gas mainly exist in the gas phase and the low molecular weight PAHs are the predominant congener. The co-processing sewage sludge results in the increase in PAHs and heavy metals emissions, especially high molecular weight PAHs and low-volatile heavy metals such as Cd and Pb in the particle phase, while it does not change their compositions and distribution patterns significantly. The concentrations and their distributions of the PAHs and heavy metals between the emissions and ambient air have a positive correlation and the co-processing sewage sludge results in the increase of PAHs and heavy metals concentrations in the ambient air. The PAHs concentration level and their distribution in soil are proportional to those in the particle phase of flue gas, and the co-processing sewage sludge can accelerate the accumulation of the PAHs and heavy metals in the surrounding soil, especially high/middle molecular weight PAHs and low-volatile heavy metals.
Abundances of Clinically Relevant Antibiotic Resistance Genes and Bacterial Community Diversity in the Weihe River, China
Authors: Wang Xiaojuan , Gu Jie , Gao Hua , Qian Xun , Li Haichao
Published: April 2018

The spread of antibiotic resistance genes in river systems is an emerging environmental issue due to their potential threat to aquatic ecosystems and public health. In this study, we used droplet digital polymerase chain reaction (ddPCR) to evaluate pollution with clinically relevant antibiotic resistance genes (ARGs) at 13 monitoring sites along the main stream of the Weihe River in China. Six clinically relevant ARGs and a class I integron-integrase (intI1) gene were analyzed using ddPCR, and the bacterial community was evaluated based on the bacterial 16S rRNA V3–V4 regions using MiSeq sequencing. The results indicated Proteobacteria, Actinobacteria, Cyanobacteria, and Bacteroidetes as the dominant phyla in the water samples from the Weihe River. Higher abundances of blaTEM, strB, aadA, and intI1 genes (103 to 105 copies/mL) were detected in the surface water samples compared with the relatively low abundances of strA, mecA, and vanA genes (0–1.94 copies/mL). Eight bacterial genera were identified as possible hosts of the intI1 gene and three ARGs (strA, strB, and aadA) based on network analysis. The results suggested that the bacterial community structure and horizontal gene transfer were associated with the variations in ARGs.
Association between Precipitation and Diarrheal Disease in Mozambique
Authors: Horn Lindsay , Hajat Anjum , Sheppard Lianne , Quinn Colin , Colborn James , Zermoglio Maria , Gudo Eduardo , Marrufo Tatiana , Ebi Kristie
Published: April 2018

Diarrheal diseases are a leading cause of morbidity and mortality in Africa. Although research documents the magnitude and pattern of diarrheal diseases are associated with weather in particular locations, there is limited quantification of this association in sub-Saharan Africa and no studies conducted in Mozambique. Our study aimed to determine whether variation in diarrheal disease was associated with precipitation in Mozambique. In secondary analyses we investigated the associations between temperature and diarrheal disease. We obtained weekly time series data for weather and diarrheal disease aggregated at the administrative district level for 1997–2014. Weather data include modeled estimates of precipitation and temperature. Diarrheal disease counts are confirmed clinical episodes reported to the Mozambique Ministry of Health (n = 7,315,738). We estimated the association between disease counts and precipitation, defined as the number of wet days (precipitation > 1 mm) per week, for the entire country and for Mozambique’s four regions. We conducted time series regression analyses using an unconstrained distributed lag Poisson model adjusted for time, maximum temperature, and district. Temperature was similarly estimated with adjusted covariates. Using a four-week lag, chosen a priori, precipitation was associated with diarrheal disease. One additional wet day per week was associated with a 1.86% (95% CI: 1.05–2.67%), 1.37% (95% CI: 0.70–2.04%), 2.09% (95% CI: 1.01–3.18%), and 0.63% (95% CI: 0.11–1.14%) increase in diarrheal disease in Mozambique’s northern, central, southern, and coastal regions, respectively. Our study indicates a strong association between diarrheal disease and precipitation. Diarrheal disease prevention efforts should target areas forecast to experience increased rainfall. The burden of diarrheal disease may increase with increased precipitation associated with climate change, unless additional health system interventions are undertaken.
Heavy Metal Pollution Delineation Based on Uncertainty in a Coastal Industrial City in the Yangtze River Delta, China
Authors: Hu Bifeng , Zhao Ruiying , Chen Songchao , Zhou Yue , Jin Bin , Li Yan , Shi Zhou
Published: April 2018

Assessing heavy metal pollution and delineating pollution are the bases for evaluating pollution and determining a cost-effective remediation plan. Most existing studies are based on the spatial distribution of pollutants but ignore related uncertainty. In this study, eight heavy-metal concentrations (Cr, Pb, Cd, Hg, Zn, Cu, Ni, and Zn) were collected at 1040 sampling sites in a coastal industrial city in the Yangtze River Delta, China. The single pollution index (PI) and Nemerow integrated pollution index (NIPI) were calculated for every surface sample (0–20 cm) to assess the degree of heavy metal pollution. Ordinary kriging (OK) was used to map the spatial distribution of heavy metals content and NIPI. Then, we delineated composite heavy metal contamination based on the uncertainty produced by indicator kriging (IK). The results showed that mean values of all PIs and NIPIs were at safe levels. Heavy metals were most accumulated in the central portion of the study area. Based on IK, the spatial probability of composite heavy metal pollution was computed. The probability of composite contamination in the central core urban area was highest. A probability of 0.6 was found as the optimum probability threshold to delineate polluted areas from unpolluted areas for integrative heavy metal contamination. Results of pollution delineation based on uncertainty showed the proportion of false negative error areas was 6.34%, while the proportion of false positive error areas was 0.86%. The accuracy of the classification was 92.80%. This indicated the method we developed is a valuable tool for delineating heavy metal pollution.
The Impact of Capital Structure on Risk and Firm Performance: Empirical Evidence for the Bucharest Stock Exchange Listed Companies
Authors: Nenu Elena , Vintilă Georgeta , Gherghina Ştefan
Published: April 2018

This paper analyzes the evolution of the main theories regarding the capital structure and the related impact on risk and corporate performance. The capital structure is a dynamic process that changes over time, depending on the variables that influence the overall evolution of the economy, a particular sector, or a company. It may also change depending on the company’s forecasts of its expected profitability, capital structure being, in fact, a risk–return compromise. This study contributes to the literature by investigating the drivers of capital structure of the firms from the Romanian market. For the econometric analysis, we applied multivariate fixed-effects regressions, as well as dynamic panel-data estimations (two-step system generalized method of moments, GMM) on a panel comprising the companies listed on the Bucharest Stock Exchange. The analyzed period, 2000–2016, covers a cycle with significant changes in the Romanian economy. Our results showed that leverage is positively correlated with the size of the company and the share price volatility. On the other hand, the debt structure has a different impact on corporate performance, whether this calculated on accounting measures or seen as market share price evolution.
Improving ASTER GDEM Accuracy Using Land Use-Based Linear Regression Methods: A Case Study of Lianyungang, East China
Authors: Yang Xiaoyan , Li Long , Chen Longgao , Chen Longqian , Shen Zhengping
Published: April 2018

The Advanced Spaceborne Thermal-Emission and Reflection Radiometer Global Digital Elevation Model (ASTER GDEM) is important to a wide range of geographical and environmental studies. Its accuracy, to some extent associated with land-use types reflecting topography, vegetation coverage, and human activities, impacts the results and conclusions of these studies. In order to improve the accuracy of ASTER GDEM prior to its application, we investigated ASTER GDEM errors based on individual land-use types and proposed two linear regression calibration methods, one considering only land use-specific errors and the other considering the impact of both land-use and topography. Our calibration methods were tested on the coastal prefectural city of Lianyungang in eastern China. Results indicate that (1) ASTER GDEM is highly accurate for rice, wheat, grass and mining lands but less accurate for scenic, garden, wood and bare lands; (2) despite improvements in ASTER GDEM2 accuracy, multiple linear regression calibration requires more data (topography) and a relatively complex calibration process; (3) simple linear regression calibration proves a practicable and simplified means to systematically investigate and improve the impact of land-use on ASTER GDEM accuracy. Our method is applicable to areas with detailed land-use data based on highly accurate field-based point-elevation measurements.
Global Transcriptome Analysis of Brown Adipose Tissue of Diet-Induced Obese Mice
Authors: Cao Jingyi , Zhu Qi , Liu Lin , Glazier Bradley , Hinkel Benjamin , Liang Chun , Shi Haifei
Published: April 2018

Consumption of a high-fat diet (HFD) promotes the development of obesity, a disease resulting from an imbalance between energy intake and energy expenditure. Brown adipose tissue (BAT) has thermogenic capacity that burns calories to produce heat, and it is a potential target for the treatment and prevention of obesity. There is limited information regarding the impact of HFD on the BAT transcriptome. We hypothesized that HFD-induced obesity would lead to transcriptional regulation of BAT genes. RNA sequencing was used to generate global transcriptome profiles from BAT of lean mice fed with a low-fat diet (LFD) and obese mice fed with a HFD. Gene Ontology (GO) analysis identified increased expression of genes involved in biological processes (BP) related to immune responses, which enhanced molecular function (MF) in chemokine activity; decreased expression of genes involved in BP related to ion transport and muscle structure development, which reduced MF in channel and transporter activity and structural binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional pathway analysis indicated that pathways associated with innate immunity were enhanced by HFD, while pathways associated with muscle contraction and calcium signaling were suppressed by HFD. Collectively, these results suggest that diet-induced obesity changes transcriptomic signatures of BAT, leading to dysfunction involving inflammation, calcium signaling, ion transport, and cell structural development.
Novel Nanoparticulate and Ionic Titanium Antigens for Hypersensitivity Testing
Authors: Høl Paul , Kristoffersen Einar , Gjerdet Nils , Pellowe Amanda
Published: April 2018

Titanium is used in a wide variety of materials ranging from medical devices to materials used in everyday life. Adverse biological reactions that could occur in patients, consumers, and workers should be monitored and prevented. There is a lack of available agents to test and predict titanium-related hypersensitivity. The aim of this study was to develop two bioavailable titanium substances in ionic and nanoparticulate form to serve as antigens for hypersensitivity testing in vitro. Peripheral blood mononuclear cells from 20 test subjects were stimulated with the antigens and secretion of monocytic and lymphatic cytokines and chemokines were measured by a multiplex bead assay. Lymphocyte stimulation indices were also determined in a subset of test subjects by measuring CD69 and HLA-DR expression by flow cytometry. Cytokine profiling revealed that both antigens increased production of typical monocyte and macrophage secreted cytokines after 24 h, with significant increases in IL-1β, IL-7, IL-10, IL-12, IL-2R, IL-6, GM-CSF, TNF-α, IL-1RA, MIP-1α, MIP-1β, IFN-α, and IL-15. Lymphatic cytokines and chemokines were not significantly induced by activation. After seven days of stimulation, ionic-Ti (2.5 μg/mL) caused proliferation (stimulation index > 2) of CD4+ cells and CD8+ cells in all persons tested (N = 6), while titanium dioxide nanoparticles (50 μg/mL) only caused significant proliferation of CD4+ cells. Our preliminary results show that the experimental titanium antigens, especially the ionic form, induce a general inflammatory response in vitro. A relevant cohort of test subjects is required to further elucidate their potential for predictive hypersensitivity testing.
Molecular Consortia—Various Structural and Synthetic Concepts for More Effective Therapeutics Synthesis
Authors: Pawełczyk Anna , Sowa-Kasprzak Katarzyna , Olender Dorota , Zaprutko Lucjusz
Published: April 2018

The design and discovery of novel drug candidates are the initial and most probably the crucial steps in the drug development process. One of the tasks of medicinal chemistry is to produce new molecules that have a desired biological effect. However, even today the search for new pharmaceuticals is a very complicated process that is hard to rationalize. Literature provides many scientific reports on future prospects of design of potentially useful drugs. Many trends have been proposed for the design of new drugs containing different structures (dimers, heterodimers, heteromers, adducts, associates, complexes, biooligomers, dendrimers, dual-, bivalent-, multifunction drugs and codrugs, identical or non-identical twin drugs, mixed or combo drugs, supramolecular particles and various nanoindividuals. Recently much attention has been paid to different strategies of molecular hybridization. In this paper, various molecular combinations were described e.g., drug–drug or drug-non-drug combinations which are expressed in a schematic multi-factor form called a molecular matrix, consisting of four factors: association mode, connection method, and the number of elements and linkers. One of the most popular trends is to create small–small molecule combinations such as different hybrids, codrugs, drug–drug conjugates (DDCs) and small-large molecule combinations such as antibody-drug conjugates (ADCs), polymer-drug conjugates (PDCs) or different prodrugs and macromolecular therapeutics. A review of the structural possibilities of active framework combinations indicates that a wide range of potentially effective novel-type compounds can be formed. What is particularly important is that new therapeutics can be obtained in fast, efficient, and selective methods using current trends in chemical synthesis and the design of drugs such as the “Lego” concept or rational green approach.
A Single Dose of Atorvastatin Applied Acutely after Spinal Cord Injury Suppresses Inflammation, Apoptosis, and Promotes Axon Outgrowth, Which Might Be Essential for Favorable Functional Outcome
Authors: Bimbova Katarina , Bacova Maria , Kisucka Alexandra , Pavel Jaroslav , Galik Jan , Zavacky Peter , Marsala Martin , Stropkovska Andrea , Fedorova Jana , Papcunova Stefania , Jachova Jana , Lukacova Nadezda
Published: April 2018

The aim of our study was to limit the inflammatory response after a spinal cord injury (SCI) using Atorvastatin (ATR), a potent inhibitor of cholesterol biosynthesis. Adult Wistar rats were divided into five experimental groups: one control group, two Th9 compression (40 g/15 min) groups, and two Th9 compression + ATR (5 mg/kg, i.p.) groups. The animals survived one day and six weeks. ATR applied in a single dose immediately post-SCI strongly reduced IL-1β release at 4 and 24 h and considerably reduced the activation of resident cells at one day post-injury. Acute ATR treatment effectively prevented the excessive infiltration of destructive M1 macrophages cranially, at the lesion site, and caudally (by 66%, 62%, and 52%, respectively) one day post-injury, whereas the infiltration of beneficial M2 macrophages was less affected (by 27%, 41%, and 16%). In addition, at the same time point, ATR visibly decreased caspase-3 cleavage in neurons, astrocytes, and oligodendrocytes. Six weeks post-SCI, ATR increased the expression of neurofilaments in the dorsolateral columns and Gap43-positive fibers in the lateral columns around the epicenter, and from day 30 to 42, significantly improved the motor activity of the hindlimbs. We suggest that early modulation of the inflammatory response via effects on the M1/M2 macrophages and the inhibition of caspase-3 expression could be crucial for the functional outcome.
Photodynamic Effect of Methylene Blue and Low Level Laser Radiation in Head and Neck Squamous Cell Carcinoma Cell Lines
Authors: Kofler Barbara , Romani Angela , Pritz Christian , Steinbichler Teresa , Schartinger Volker , Riechelmann Herbert , Dudas Jozsef
Published: April 2018

Photodynamic therapy (PDT) is suggested to have an impact on the treatment of early stage head and neck cancers (HNSCC). We investigated the effect of PDT with methylene blue (MB) and a diode laser (660 nm) as the laser source on HNSCC cell lines as an in vitro model of surface oral squamous cell carcinoma. Cell-cultures were exposed to 160 µM MB for 4 min and to laser light for 8 min. Viability was proven via cell viability assay and clonogenic survival via clone counting assay. The combination of MB and diode laser evidenced high efficient loss of cell viability by 5% of the control, while treatment with the same concentration of MB for 4 min alone showed a viability of 46% of the control. In both SCC-25 and Detroit 562 HNSCC cells, MB combined with the laser allowed a significant abrogation of clonogenic growth (p < 0.01), especially in the case of Detroit 562 cells less than 1% of the suspension plated cells were able to grow tumor cell nests. Multiresistant (Detroit 562) HNSCC cells expressing cancer stem cell markers are sensitive to MB/red laser combined PDT.
Dandelion Root Extract Induces Intracellular Ca2+ Increases in HEK293 Cells
Authors: Gerbino Andrea , Russo Daniela , Colella Matilde , Procino Giuseppe , Svelto Maria , Milella Luigi , Carmosino Monica
Published: April 2018

Dandelion (Taraxacum officinale Weber ex F.H.Wigg.) has been used for centuries as an ethnomedical remedy. Nonetheless, the extensive use of different kinds of dandelion extracts and preparations is based on empirical findings. Some of the tissue-specific effects reported for diverse dandelion extracts may result from their action on intracellular signaling cascades. Therefore, the aim of this study was to evaluate the effects of an ethanolic dandelion root extract (DRE) on Ca2+ signaling in human embryonic kidney (HEK) 293 cells. The cytotoxicity of increasing doses of crude DRE was determined by the Calcein viability assay. Fura-2 and the fluorescence resonance energy transfer (FRET)-based probe ERD1 were used to measure cytoplasmic and intraluminal endoplasmic reticulum (ER) Ca2+ levels, respectively. Furthermore, a green fluorescent protein (GFP)-based probe was used to monitor phospholipase C (PLC) activation (pleckstrin homology [PH]–PLCδ–GFP). DRE (10–400 µg/mL) exposure, in the presence of external Ca2+, dose-dependently increased intracellular Ca2+ levels. The DRE-induced Ca2+ increase was significantly reduced in the absence of extracellular Ca2+. In addition, DRE caused a significant Ca2+ release from the ER of intact cells and a concomitant translocation of PH–PLCδ–GFP. In conclusion, DRE directly activates both the release of Ca2+ from internal stores and a significant Ca2+ influx at the plasma membrane. The resulting high Ca2+ levels within the cell seem to directly stimulate PLC activity.
DDX5 RNA Helicases: Emerging Roles in Viral Infection
Authors: Cheng Wenyu , Chen Guohua , Jia Huaijie , He Xiaobing , Jing Zhizhong
Published: April 2018

Asp-Glu-Ala-Asp (DEAD)-box polypeptide 5 (DDX5), also called p68, is a prototypical member of the large ATP-dependent RNA helicases family and is known to participate in all aspects of RNA metabolism ranging from transcription to translation, RNA decay, and miRNA processing. The roles of DDX5 in cell cycle regulation, tumorigenesis, apoptosis, cancer development, adipogenesis, Wnt-β-catenin signaling, and viral infection have been established. Several RNA viruses have been reported to hijack DDX5 to facilitate various steps of their replication cycles. Furthermore, DDX5 can be bounded by the viral proteins of some viruses with unknown functions. Interestingly, an antiviral function of DDX5 has been reported during hepatitis B virus and myxoma virus infection. Thus, the precise roles of this apparently multifaceted protein remain largely obscure. Here, we provide a rapid and critical overview of the structure and functions of DDX5 with a particular emphasis on its role during virus infection.
β-Nicotinamide Adenine Dinucleotide (β-NAD) Inhibits ATP-Dependent IL-1β Release from Human Monocytic Cells
Authors: Hiller Sebastian , Heldmann Sarah , Richter Katrin , Jurastow Innokentij , Küllmar Mira , Hecker Andreas , Wilker Sigrid , Fuchs-Moll Gabriele , Manzini Ivan , Schmalzing Günther , Kummer Wolfgang , Padberg Winfried , McIntosh J. , Damm Jelena , Zakrzewicz Anna , Grau Veronika
Published: April 2018

While interleukin-1β (IL-1β) is a potent pro-inflammatory cytokine essential for host defense, high systemic levels cause life-threatening inflammatory syndromes. ATP, a stimulus of IL-1β maturation, is released from damaged cells along with β-nicotinamide adenine dinucleotide (β-NAD). Here, we tested the hypothesis that β-NAD controls ATP-signaling and, hence, IL-1β release. Lipopolysaccharide-primed monocytic U937 cells and primary human mononuclear leukocytes were stimulated with 2′(3′)-O-(4-benzoyl-benzoyl)ATP trieethylammonium salt (BzATP), a P2X7 receptor agonist, in the presence or absence of β-NAD. IL-1β was measured in cell culture supernatants. The roles of P2Y receptors, nicotinic acetylcholine receptors (nAChRs), and Ca2+-independent phospholipase A2 (iPLA2β, PLA2G6) were investigated using specific inhibitors and gene-silencing. Exogenous β-NAD signaled via P2Y receptors and dose-dependently (IC50 = 15 µM) suppressed the BzATP-induced IL-1β release. Signaling involved iPLA2β, release of a soluble mediator, and nAChR subunit α9. Patch-clamp experiments revealed that β-NAD inhibited BzATP-induced ion currents. In conclusion, we describe a novel triple membrane-passing signaling cascade triggered by extracellular β-NAD that suppresses ATP-induced release of IL-1β by monocytic cells. This cascade links activation of P2Y receptors to non-canonical metabotropic functions of nAChRs that inhibit P2X7 receptor function. The biomedical relevance of this mechanism might be the control of trauma-associated systemic inflammation.
Irradiation-Induced Cardiac Connexin-43 and miR-21 Responses Are Hampered by Treatment with Atorvastatin and Aspirin
Authors: Viczenczova Csilla , Kura Branislav , Egan Benova Tamara , Yin Chang , Kukreja Rakesh , Slezak Jan , Tribulova Narcis , Szeiffova Bacova Barbara
Published: April 2018

Radiation of the chest during cancer therapy is deleterious to the heart, mostly due to oxidative stress and inflammation related injury. A single sub-lethal dose of irradiation has been shown to result in compensatory up-regulation of the myocardial connexin-43 (Cx43), activation of the protein kinase C (PKC) signaling along with the decline of microRNA (miR)-1 and an increase of miR-21 levels in the left ventricle (LV). We investigated whether drugs with antioxidant, anti-inflammatory or vasodilating properties, such as aspirin, atorvastatin, and sildenafil, may affect myocardial response in the LV and right ventricle (RV) following chest irradiation. Adult, male Wistar rats were subjected to a single sub-lethal dose of chest radiation at 25 Gy and treated with aspirin (3 mg/day), atorvastatin (0.25 mg/day), and sildenafil (0.3 mg/day) for six weeks. Cx43, PKCε and PKCδ proteins expression and levels of miR-1 as well as miR-21 were determined in the LV and RV. Results showed that the suppression of miR-1 was associated with an increase of total and phosphorylated forms of Cx43 as well as PKCε expression in the LV while having no effect in the RV post-irradiation as compared to the non-irradiated rats. Treatment with aspirin and atorvastatin prevented an increase in the expression of Cx43 and PKCε without change in the miR-1 levels. Furthermore, treatment with aspirin, atorvastatin, and sildenafil completely prevented an increase of miR-21 in the LV while having partial effect in the RV post irradiation. The increase in pro-apoptotic PKCδ was not affected by any of the used treatment. In conclusion, irradiation and drug-induced changes were less pronounced in the RV as compared to the LV. Treatment with aspirin and atorvastatin interfered with irradiation-induced compensatory changes in myocardial Cx43 protein and miR-21 by preventing their elevation, possibly via amelioration of oxidative stress and inflammation.
Assessment of Ab Initio and Density Functional Theory Methods for the Excitations of Donor-Acceptor Complexes: The Case of the Benzene-Tetracyanoethylene Model
Authors: Xu Peng , Zhang Cai-Rong , Wang Wei , Gong Ji-Jun , Liu Zi-Jiang , Chen Hong-Shan
Published: April 2018

The understanding of the excited-state properties of electron donors, acceptors and their interfaces in organic optoelectronic devices is a fundamental issue for their performance optimization. In order to obtain a balanced description of the different excitation types for electron-donor-acceptor systems, including the singlet charge transfer (CT), local excitations, and triplet excited states, several ab initio and density functional theory (DFT) methods for excited-state calculations were evaluated based upon the selected model system of benzene-tetracyanoethylene (B-TCNE) complexes. On the basis of benchmark calculations of the equation-of-motion coupled-cluster with single and double excitations method, the arithmetic mean of the absolute errors and standard errors of the electronic excitation energies for the different computational methods suggest that the M11 functional in DFT is superior to the other tested DFT functionals, and time-dependent DFT (TDDFT) with the Tamm–Dancoff approximation improves the accuracy of the calculated excitation energies relative to that of the full TDDFT. The performance of the M11 functional underlines the importance of kinetic energy density, spin-density gradient, and range separation in the development of novel DFT functionals. According to the TDDFT results, the performances of the different TDDFT methods on the CT properties of the B-TCNE complexes were also analyzed.
The Role of Extracellular Vesicles in Bone Metastasis
Authors: Rossi Michela , Battafarano Giulia , D’Agostini Matteo , Del Fattore Andrea
Published: April 2018

Multiple types of cancer have the specific ability to home to the bone microenvironment and cause metastatic lesions. Despite being the focus of intense investigation, the molecular and cellular mechanisms that regulate the metastasis of disseminated tumor cells still remain largely unknown. Bone metastases severely impact quality of life since they are associated with pain, fractures, and bone marrow aplasia. In this review, we will summarize the recent discoveries on the role of extracellular vesicles (EV) in the regulation of bone remodeling activity and bone metastasis occurrence. Indeed, it was shown that extracellular vesicles, including exosomes and microvesicles, released from tumor cells can modify the bone microenvironment, allowing the formation of osteolytic, osteosclerotic, and mixed mestastases. In turn, bone-derived EV can stimulate the proliferation of tumor cells. The inhibition of EV-mediated crosstalk between cancer and bone cells could represent a new therapeutic target for bone metastasis.
Rhodobacter sphaeroides Extract Lycogen™ Attenuates Testosterone-Induced Benign Prostate Hyperplasia in Rats
Authors: Wang Chiang-Ting , Wang Ya-Yun , Liu Wen-Sheng , Cheng Chun-Ming , Chiu Kuo-Hsun , Liu Li-Lian , Liu Xue-Zhu , Wen Zhi-Hong , Chen Ya-Huey , Chen Tsung-Ming
Published: April 2018

Benign prostate hyperplasia (BPH) is one of the most common urological problems in mid-aged to elderly men. Risk factors of BPH include family history, obesity, type 2 diabetes, and high oxidative stress. The main medication classes for BPH management are alpha blockers and 5α-reductase inhibitors. However, these conventional medicines cause adverse effects. Lycogen™, extracted from Rhodobacter sphaeroides WL-APD911, is an anti-oxidant and anti-inflammatory compound. In this study, the effect of Lycogen™ was evaluated in rats with testosterone-induced benign prostate hyperplasia (BPH). Testosterone injections and Lycogen™ administration were carried out for 28 days, and body weights were recorded twice per week. The testosterone injection successfully induced a prostate enlargement. BPH-induced rats treated with different doses of Lycogen™ exhibited a significantly decreased prostate index (PI). Moreover, the Lycogen™ administration recovered the histological abnormalities observed in the prostate of BPH rats. In conclusion, these findings support a dose-dependent preventing effect of Lycogen™ on testosterone-induced BPH in rats and suggest that Lycogen™ may be favorable to the prevention and management of benign prostate hyperplasia.
A Review of the Interactions between Wheat and Wheat Pathogens: Zymoseptoria tritici, Fusarium spp. and Parastagonospora nodorum
Authors: Duba Adrian , Goriewa-Duba Klaudia , Wachowska Urszula
Published: April 2018

Zymoseptoria tritici is a hemibiotrophic pathogen which causes Septoria leaf blotch in wheat. The pathogenesis of the disease consists of a biotrophic phase and a necrotrophic phase. The pathogen infects the host plant by suppressing its immune response in the first stage of infection. Hemibiotrophic pathogens of the genus Fusarium cause Fusarium head blight, and the necrotrophic Parastagonospora nodorum is responsible for Septoria nodorum blotch in wheat. Cell wall-degrading enzymes in plants promote infections by necrotrophic and hemibiotrophic pathogens, and trichothecenes, secondary fungal metabolites, facilitate infections caused by fungi of the genus Fusarium. There are no sources of complete resistance to the above pathogens in wheat. Defense mechanisms in wheat are controlled by many genes encoding resistance traits. In the wheat genome, the characteristic features of loci responsible for resistance to pathogenic infections indicate that at least several dozen genes encode resistance to pathogens. The molecular interactions between wheat and Z. tritici, P. nodorum and Fusarium spp. pathogens have been insufficiently investigated. Most studies focus on the mechanisms by which the hemibiotrophic Z. tritici suppresses immune responses in plants and the role of mycotoxins and effector proteins in infections caused by P. nodorum and Fusarium spp. fungi. Trichothecene glycosylation and effector proteins, which are involved in defense responses in wheat, have been described at the molecular level. Recent advances in molecular biology have produced interesting findings which should be further elucidated in studies of molecular interactions between wheat and fungal pathogens. The Clustered Regularly-Interspaced Short Palindromic Repeats/ CRISPR associated (CRISPR/Cas) system can be used to introduce targeted mutations into the wheat genome and confer resistance to selected fungal diseases. Host-induced gene silencing and spray-induced gene silencing are also useful tools for analyzing wheat–pathogens interactions which can be used to develop new strategies for controlling fungal diseases.
Structural Requirements of Alkylglyceryl-l-Ascorbic Acid Derivatives for Melanogenesis Inhibitory Activity
Authors: Taira Norihisa , Katsuyama Yushi , Yoshioka Masato , Muraoka Osamu , Morikawa Toshio
Published: April 2018

l-Ascorbic acid has multifunctional benefits on skin aesthetics, including inhibition of melanin production, and is widely used in cosmetics. It, however, has low stability and poor skin penetration. We hypothesize that alkylglyceryl-l-ascorbic acid derivatives, highly stable vitamin C–alkylglycerol conjugates, would have similar anti-melanogenic activity with better stability and penetration. We test 28 alkylglyceryl-l-ascorbic acid derivatives (1–28) on theophylline-stimulated B16 melanoma 4A5 cells to determine if they inhibit melanogenesis and establish any structure–function relationships. Although not the most potent inhibitors, 3-O-(2,3-dihydroxypropyl)-2-O-hexyl-l-ascorbic acid (6, IC50 = 81.4 µM) and 2-O-(2,3-dihydroxypropyl)-3-O-hexyl-l-ascorbic acid (20, IC50 = 117 µM) are deemed the best candidate derivatives based on their inhibitory activities and low toxicities. These derivatives are also found to be more stable than l-ascorbic acid and to have favorable characteristics for skin penetration. The following structural requirements for inhibitory activity of alkylglyceryl-l-ascorbic acid derivatives are also determined: (i) alkylation of glyceryl-l-ascorbic acid is essential for inhibitory activity; (ii) the 3-O-alkyl-derivatives (2–14) exhibit stronger inhibitory activity than the corresponding 2-O-alkyl-derivatives (16–28); and (iii) derivatives with longer alkyl chains have stronger inhibitory activities. Mechanistically, our studies suggest that l-ascorbic acid derivatives exert their effects by suppressing the mRNA expression of tyrosinase and tyrosine-related protein-1.
Thinking in Patterns and the Pattern of Human Thought as Contrasted with AI Data Processing
Authors: Logan Robert , Tandoc Marlie
Published: April 2018

We propose that the ability of humans to identify and create patterns led to the unique aspects of human cognition and culture as a complex emergent dynamic system consisting of the following human traits: patterning, social organization beyond that of the nuclear family that emerged with the control of fire, rudimentary set theory or categorization and spoken language that co-emerged, the ability to deal with information overload, conceptualization, imagination, abductive reasoning, invention, art, religion, mathematics and science. These traits are interrelated as they all involve the ability to flexibly manipulate information from our environments via pattern restructuring. We argue that the human mind is the emergent product of a shift from external percept-based processing to a concept and language-based form of cognition based on patterning. In this article, we describe the evolution of human cognition and culture, describing the unique patterns of human thought and how we, humans, think in terms of patterns.
A Simple ImageJ-Based Method to Measure Cardiac Rhythm in Zebrafish Embryos
Authors: Sampurna Bonifasius , Audira Gilbert , Juniardi Stevhen , Lai Yu-Heng , Hsiao Chung-Der
Published: April 2018

Zebrafish has an advantage of optical clarity within the body during its early developmental stage. Therefore, organs, including the heart, can be observed and analyzed in a non-invasive manner. Previously, heart rate measurement was analyzed by a complicated system, programming language, fluorescence transgenic zebrafish, or expensive setup that was not feasible and practical. In this study, we established a simple dissecting microscope with Charge-coupled Device (CCD) to capture the signal of heartbeat, and used ImageJ software as an open source platform to analyze cardiac rhythm for the atrium and the ventricle. We subjected astemizole to zebrafish embryos, and applied our apparatus to precisely detect heart arrhythmia (Atrium–Ventricle block) induction. In other words, we provided a simple, economical, and reliable method to measure cardiac rhythm in zebrafish embryos, which is able to evaluate whether cardiac rhythm is affected by chemical compounds.