bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Adenosine in Overcoming Resistance in Sarcomas
Authors: Cruz-Ramos Marlid , Cabrera-Nieto Sara Aileen , Murguia-Perez Mario , Fajardo-Espinoza Fernanda Sarahí
Published: November 2024

Resistance to systemic therapies in sarcomas poses a significant challenge to improving clinical outcomes. Recent research has concentrated on the tumor microenvironment’s role in sarcoma progression and treatment resistance. This microenvironment comprises a variety of cell types and signaling molecules that influence tumor behavior, including proliferation, metastasis, and resistance to therapy. Adenosine, abundant in the tumor microenvironment, has been implicated in promoting immunosuppression and chemoresistance. Targeting adenosine receptors and associated pathways offers a novel approach to enhancing immune responses against tumors, potentially improving immunotherapy outcomes in cancers, including sarcomas. Manipulating adenosine signaling also shows promise in overcoming chemotherapy resistance in these tumors. Clinical trials investigating adenosine receptor antagonists in sarcomas have fueled interest in this pathway for sarcoma treatment. Ultimately, a comprehensive understanding of the tumor and vascular microenvironments, as well as the adenosine pathway, may open new avenues for improving treatment outcomes and overcoming resistance in sarcoma. Further studies and clinical trials are crucial to validate these findings and optimize therapeutic strategies, particularly for osteosarcoma. This study provides a literature review exploring the potential role of the adenosine pathway in sarcomas.
Interleukin-6 Modulates the Expression and Function of HCN Channels: A Link Between Inflammation and Atrial Electrogenesis
Authors: Spinelli Valentina , Laurino Annunziatina , Balducci Valentina , Gencarelli Manuela , Ruzzolini Jessica , Nediani Chiara , Mandoli Giulia Elena , Cameli Matteo , Sacconi Leonardo , Sartiani Laura , Cerbai Elisabetta
Published: November 2024

Inflammatory cytokines, including interleukin 6 (IL6), are associated with ion channel remodeling and enhance the propensity to alterations in cardiac rhythm generation and propagation, in which the hyperpolarization-activated cyclic nucleotide-gated (HCN) channels play a crucial role. Hence, we investigated the consequences of exposure to IL6 on HCN channels in cell models and human atrial biopsies. In murine atrial HL1 cells and in cardiomyocytes derived from human induced pluripotent stem cells (hiPS-CMs), IL6 elicited STAT3 phosphorylation, a receptor-mediated downstream signaling. Downregulation of HCN1,2,4 by IL6 was observed after 24–48 h; in hiPS-CMs, this effect was reverted by 24 h of application of tocilizumab, a human IL6 receptor antagonist. In parallel, hiPS-CM action potentials (APs) showed a reduced spontaneous frequency. Moreover, we assessed IL6 and HCN expression in dilated left atrial samples from patients with mitral valve disease, an AF-prone condition. IL6 levels were increased in dilated atria compared to controls and positively correlated with echocardiographic atrial dimensions. Interestingly, the highest IL6 transcript levels and the lowest HCN4 and HCN2 expression were in these samples. In conclusion, our data uncovered a novel link between IL6 and cardiac HCN channels, potentially contributing to atrial electrical disturbances and a higher risk of dysrhythmias in conditions with elevated IL6 levels.
Ontogeny of Thyroid Hormone Signaling in the Retina of Zebrafish: Effects of Thyroidal Status on Retinal Morphology, Cell Survival, and Color Preference
Authors: Lazcano Iván , Pech-Pool Santiago M. , Maldonado-Lira María Fernanda , Olvera Aurora , Darras Veerle M. , Orozco Aurea
Published: November 2024

The retina is crucial for converting light into neuronal signals for visual perception. Understanding the retina’s structure, function, and development is essential for vision research. It is known that the thyroid hormone (TH) receptor type beta 2 (TRβ2) is a key element in the regulation of cone differentiation in the retina, but other elements of TH signaling, such as transporters and enzyme deiodinases, have also been implicated in retinal cell development and survival. In the present study, we investigated the expression profile of genes involved in TH signaling and analyzed the impact of thyroidal status on retinal morphology, opsin expression, cell death/proliferation profile, as well as color preference behavior during the early retina development of zebrafish larvae. mRNA expression analysis on dissected whole eyes revealed that TH signaling elements gradually increase during eye development, with dio3b being the component that shows the most dramatic change. Mutations generated by CRISPR/CAS9 in the dio3b gene, but not in the thrb gene, modifies the structure of the retina. Disruption in TH level reduces the cell number of the ganglion cell layer, increases cell death, and modifies color preference, emphasizing the critical importance of precise TH regulation by its signaling elements for optimal retinal development and function.
Detection of Potato Pathogen Clavibacter sepedonicus by CRISPR/Cas13a Analysis of NASBA Amplicons
Authors: Khmeleva Svetlana A. , Kurbatov Leonid K. , Ptitsyn Konstantin G. , Timoshenko Olga S. , Morozova Darya D. , Suprun Elena V. , Radko Sergey P. , Lisitsa Andrey V.
Published: November 2024

The ring rot of potato caused by the bacterial pathogen Clavibacter sepedonicus is a quarantine disease posing a threat to the potato industry worldwide. The sensitive and selective detection of C. sepedonicus is of a high importance for its effective control. Here, the detection system is reported to determine viable bacteria of C. sepedonicus in potato tubers, based on the coupling of CRISPR/Cas13a nuclease with NASBA (Nucleic Acid Sequence Based Amplification)—the method of isothermal amplification of RNA. Detection can be conducted using both instrumental and non-instrumental (visual inspection of test tubes under blue light) modes. When NASBA and Cas13a analyses were carried out in separate test tubes, the limit of detection (LOD) for the system was 1000 copies of purified target 16S rRNA per NASBA reaction or about 24 colony-forming units (CFUs) of C. sepedonicus per 1 g of tuber tissue. The testing can also be conducted in the “one-pot” format (a single test tube), though with lower sensitivity: LOD was 10,000 copies of target RNA or about 100 CFU per 1 g of tuber tissue for both instrumental and visual detection modes. The overall time of NASBA/Cas13a analysis did not exceed 2 h. The developed NASBA/Cas13a detection system has the potential to be employed as a routine test of C. sepedonicus, especially for on-site testing.
Current Understanding of the Role of Autophagy in the Treatment of Myeloid Leukemia
Authors: Kubota Yasushi , Kimura Shinya
Published: November 2024

The most important issues in acute myeloid leukemia are preventing relapse and treating relapse. Although the remission rate has improved to approximately 80%, the 5-year survival rate is only around 30%. The main reasons for this are the high relapse rate and the limited treatment options. In chronic myeloid leukemia patients, when a deep molecular response is achieved for a certain period of time through tyrosine kinase inhibitor treatment, about half of them will reach treatment-free remission, but relapse is still a problem. Therefore, potential therapeutic targets for myeloid leukemias are eagerly awaited. Autophagy suppresses the development of cancer by maintaining cellular homeostasis; however, it also promotes cancer progression by helping cancer cells survive under various metabolic stresses. In addition, autophagy is promoted or suppressed in cancer cells by various genetic mutations. Therefore, the development of therapies that target autophagy is also being actively researched in the field of leukemia. In this review, studies of the role of autophagy in hematopoiesis, leukemogenesis, and myeloid leukemias are presented, and the impact of autophagy regulation on leukemia treatment and the clinical trials of autophagy-related drugs to date is discussed.
Helicobacter pylori Efflux Pumps: A Double-Edged Sword in Antibiotic Resistance and Biofilm Formation
Authors: Krzyżek Paweł
Published: November 2024

Helicobacter pylori is a major pathogen associated with various gastric diseases. Despite decades of research, the treatment of H. pylori remains challenging. One of the primary mechanisms contributing to failures of therapies targeting this bacterium is genetic mutations in drug target sites, although the growing body of scientific data highlights that efflux pumps may also take part in this process. Efflux pumps are proteinaceous transporters actively expelling antimicrobial agents from the interior of the targeted cells and reducing the intracellular concentration of these compounds. Considering that efflux pumps contribute to both antimicrobial resistance and biofilm formation, an in-depth understanding of their properties may constitute a cornerstone in the development of novel therapeutics against H. pylori. In line with this, the aim of the current review is to describe the multitude of efflux pumps produced by H. pylori and present the data describing the involvement of these proteins in tolerance and/or resistance to various classes of antimicrobial substances.
Metabolic Reprogramming of Immune Cells in the Tumor Microenvironment
Authors: Wang Jing , He Yuanli , Hu Feiming , Hu Chenchen , Sun Yuanjie , Yang Kun , Yang Shuya
Published: November 2024

Metabolic reprogramming of immune cells within the tumor microenvironment (TME) plays a pivotal role in shaping tumor progression and responses to therapy. The intricate interplay between tumor cells and immune cells within this ecosystem influences their metabolic landscapes, thereby modulating the immune evasion tactics employed by tumors and the efficacy of immunotherapeutic interventions. This review delves into the metabolic reprogramming that occurs in tumor cells and a spectrum of immune cells, including T cells, macrophages, dendritic cells, and myeloid-derived suppressor cells (MDSCs), within the TME. The metabolic shifts in these cell types span alterations in glucose, lipid, and amino acid metabolism. Such metabolic reconfigurations can profoundly influence immune cell function and the mechanisms by which tumors evade immune surveillance. Gaining a comprehensive understanding of the metabolic reprogramming of immune cells in the TME is essential for devising novel cancer therapeutic strategies. By targeting the metabolic states of immune cells, it is possible to augment their anti-tumor activities, presenting new opportunities for immunotherapeutic approaches. These strategies hold promise for enhancing treatment outcomes and circumventing the emergence of drug resistance.
Nutritional and Nutraceutical Support to the Failing Myocardium: A Possible Way of Potentiating the Current Treatment of Heart Failure
Authors: Macrì Roberta , Mollace Rocco , Serra Maria , Scarano Federica , Ritorto Giovanna , Ussia Sara , Cardamone Antonio , Coppoletta Anna Rita , Carresi Cristina , Gliozzi Micaela , Musolino Vincenzo , Maiuolo Jessica , Palma Ernesto , Volterrani Maurizio , Mollace Vincenzo , Muscoli Carolina
Published: November 2024

Heart failure (HF) is a complex condition that affects 1–2% of the global population. The presence of comorbidities like diabetes, hypertension, hyperlipidemia, or obesity has been shown in various studies to elevate mortality and hospitalization rates in HF patients. Insufficient outcomes persist in HF, necessitating additional research to address unmet needs in disease management. Lifestyle modifications, including smoking cessation, decreased alcohol consumption, regular exercise, cardiac rehabilitation, and a balanced diet, can prevent and treat a wide range of HF cases. In this review, we aimed to examine how lifestyle changes, nutrition, and nutraceutical supplements can play a role in preventing heart failure and supporting its treatment. A detailed and comprehensive analysis of the most recent data present in the literature could help identify potential candidates for future clinical trials in HF management. There is a growing body of evidence supporting the importance of closely monitoring nutritional balance, including micronutrients and nutraceuticals, in HF patients for better symptom management and outcomes. Despite promising results from initial approaches, the lack of conclusive evidence from recent studies and meta-analyses questions the widespread use of nutraceutical supplementation in HF patients. Further studies are necessary to determine the most effective way to use nutraceutical supplementation in the treatment of myocardial dysfunction in HF patients.
Non-Coding RNAs as Potential Diagnostic/Prognostic Markers for Hepatocellular Carcinoma
Authors: Tonon Federica , Grassi Chiara , Tierno Domenico , Biasin Alice , Grassi Mario , Grassi Gabriele , Dapas Barbara
Published: November 2024

The increasing incidence of hepatocellular carcinoma (HCC), together with the poor effectiveness of the available treatments, make early diagnosis and effective screening of utmost relevance. Liquid biopsy represents a potential novel approach to early HCC detection and monitoring. The identification of blood markers has many desirable features, including the absence of any significant risk for the patients, the possibility of being used as a screening tool, and the ability to perform multiple tests, thus allowing for the real-time monitoring of HCC evolution. Unfortunately, the available blood markers for HCC have several limitations, mostly related to specificity and sensitivity. In this context, employing non-coding RNAs (ncRNAs) may represent an interesting and novel diagnostic approach. ncRNAs, which include, among others, micro interfering RNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs), regulate human gene expression via interactions with their target mRNA. Notably, their expression can be altered in HCC, thus reflecting disease status. In this review, we discuss some notable works that describe the use of miRNAs, lncRNAs, and circRNAs as HCC biomarkers. Despite some open aspects related to ncRNA use, the presented works strongly support the potential effectiveness of these molecules as diagnostic/prognostic markers for HCC.
From Structure to Strength: Analyzing the Impact of Sulfuric Acid on Pig Bone Demineralization Through FTIR, LIBS, and AAS
Authors: Marković Milica , Kuzmanović Miroslav , Ranković Dragan , Bajuk-Bogdanović Danica , Šajić Aleksandra , Dimić Dušan
Published: November 2024

The present research aimed to investigate the demineralizing effects of sulfuric acid on pig bone. Alterations in collagen and phosphate contents and changes in the elemental composition of the bone during the 14-day-long immersion in sulfuric acid solutions of different concentrations were estimated using ATR-FTIR, LIBS, and AAS. FTIR spectra at amide I (1800–1600 cm−1) and phosphate ν1/ν3 (PO43−) (1300–900 cm−1) domains were scrutinized using the deconvolution method for monitoring changes in the protein secondary structure and mineral content. The results implicated sulfuric acid as a powerful demineralization agent and effective in targeting mineral components, such as hydroxyapatite, while leaving the collagen matrix relatively preserved with a complex secondary structure. Collagen maturity marker values gave valuable insights into the structural integrity of the bone. LIBS and AAS indicated changes in bone hardness; phosphorous-to-carbon ratio; and calcium, phosphorous, and magnesium content in the solutions left after the immersion period. The changes in the ratio of ionic-to-atomic calcium lines in the LIBS spectra indicated hardening of the bone, with increasing acid concentration and prolonged action, due to the deposition of calcium sulfate on the surface. The calcium concentration in the solutions decreased with increased acid concentration, while the change in phosphorus and magnesium concentrations was reversed.
Molecular Insights into the Inhibition of Lipid Accumulation in Hepatocytes by Unique Extracts of Ashwagandha
Authors: Li Dongyang , Han Hanlin , Sun Yixin , Zhang Huayue , Yoshitomi Ren , Kaul Sunil C. , Wadhwa Renu
Published: November 2024

We investigated the effect of purified withanolides and extracts derived from Ashwagandha on steatosis, the abnormal accumulation of fat that can lead to non-alcoholic fatty liver disease (NAFLD). Collaborator of ARF (CARF, also known as CDKN2AIP, a protein that regulates hepatic lipid metabolism, fat buildup, and liver damage) was used as an indicator. Six withanolides (Withaferin A, Withanone, Withanolide B, Withanoside IV, Withanoside V, and Withanostraminolide-12 deoxy) reversed the decrease in CARF caused by exposure to free fatty acids (FFAs) in liver-derived cells (HepG2 hepatocytes). After analyzing the effects of these withanolides on CARF mRNA and protein levels, FFA accumulation, protein aggregation, and oxidative and DNA damage stresses, we selected Withaferin A and Withanone for molecular analyses. Using the palmitic-acid-induced fatty acid accumulation stress model in Huh7 cells, we found a significant reduction in the activity of the key regulators of lipogenesis pathways, including sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase (FASN), and peroxisome proliferator-activated receptors (PPARγ and PPARα). This in vitro study suggests that low, non-toxic doses of Withaferin A, Withanone, or Ashwagandha extracts containing these withanolides possess anti-steatosis and antioxidative-stress properties. Further in vivo and clinical studies are required to investigate the therapeutic potential of these Ashwagandha-derived bioactive ingredients for NAFLD.
Use of Chitosan and Chitosan–Magnetite Spheres for Arsenic Groundwater Removal: Factorial Designs as Tools to Optimize the Efficiency of Removal
Authors: Hernández Trespalacios Mayra , Mangiameli María Florencia , Gribaudo Lina , Frascaroli María Inés , González Juan Carlos
Published: November 2024

The lack of access to drinking water is a problem affecting many regions worldwide. In Santa Fe, Argentina, the population uses groundwater as a source of drinking water. Unfortunately, it has high concentrations of As(V), which makes it unsuitable for consumption. Despite several methods for As(V) quantification and elimination, the high cost and technical difficulties in their implementation make many of them cost-ineffective, especially in small communities. In this work, a hybrid sorbent of magnetite–chitosan spheres (M-Q) is synthesized, and its sorption capacity is evaluated by employing groundwater with high conductivity (12.1 mS/cm) and hardness (1125 mg/L CaCO3) and an As(V) concentration of 0.265 mg/L. A colorimetric analytical technique, which is sufficiently sensitive, simple, and economical to apply, is used for As(V) quantification. The experimental results indicate that the sorption capacity for As(V) removal is 80.1% (sorbent mass 0.466 g, time 85.3 min, and pH 5.9), with the advantage of the capability of being independent of its magnetic properties. The optimal experimental conditions for As(V) sorption (pH, time, and mass of the hybrid sorbent M-Q) are obtained by response surface factorial designs, which significantly reduce the total number of experiments and, at the same time, demonstrate that all the selected variables significantly affect the As(V) sorption percentage (response studied).
Spatiotemporal Distribution of Host Plants of Dusky Cotton Bug, Oxycarenus laetus, Kirby 1891 at Different Climatic Zones of Sindh, Pakistan
Authors: Rind Muhammad , Sahito Hakim , Vono Gregorio
Published: November 2024

The study aimed to identify the host plants of the Dusky Cotton Bug (Oxycarenus laetus), in various agro-ecological zones of Sindh, Pakistan, 2019. Samples were collected bi-weekly within 20 km of the Cotton Agriculture Research Station in each district of Sindh. The pest population is categorized into three levels: below 25; 25 to 49 and 50 or more adults and nymphs. The study identified approximately 63 host plants across 31 families. The highest overall mean of pest populations was recorded on Ladyfinger (Okra), with 51.75 ± 8.15 bugs per shoot at Kotdiji and 53.71 ± 4.68 per shoot at Sakrand, both in the Malvaceae family. A high overall mean of populations was also observed on Mango (Anacardiaceae) with 51.65 ± 11.99 bugs per shoot at Kotdiji and 46.42 ± 5.84 per shoot at Sakrand on Orange (Rutaceae) with 42.07 ± 8.93 bugs per shoot at Kotdiji and 45.17 ± 4.11 per shoot at Sakrand, and on Eucalyptus and Guava (Myrtaceae) with 29.75 ± 6.76 per shoot at Kotdiji and 26.53 ± 3.71 per shoot at Tandojam, respectively. Additionally, the pest was found on Jujube (Rhamnaceae) with an overall mean population of 26.92 ± 3.52 per shoot at Sakrand The results indicate that the Dusky cotton Bug is most active at the end of summer and the beginning of winter, preferring high-opened cotton bolls during period of slightly lower temperatures and humidity for overwintering from December to March on seed-producing host plants. These findings are crucial for understanding the host plant preferences of the Dusky Cotton Bug, and for implementing effective Integrated Pest Management (IPM) strategies.
Myval Transcatheter Heart Valve: The Future of Transcatheter Valve Replacement and Significance in Current Timeline
Authors: Kilic Teoman , Coskun Senol , Mirzamidinov Didar , Yilmaz Irem , Yavuz Sadan , Sahin Tayfun
Published: November 2024

The Myval is a balloon-expandable transcatheter heart valve (THV) developed by Meril Life Sciences Pvt. Ltd. (Vapi, Gujarat, India) that has an innovative operator-friendly design that aids in improving deliverability and features precise deployment. Various clinical studies demonstrate its effectiveness and safety, making it a promising choice in valvular interventions. Myval has been successfully utilized as a transcatheter aortic valve implantation (TAVI) device in cases with conduction disturbances, bicuspid aortic valve anatomy, non-calcified aortic regurgitation, dysfunctional stenosed right ventricular outflow tract (RVOT) conduits, pulmonary valve replacement, mitral valve replacement, and valve-in-valve and valve-in-ring implantation procedures. Myval’s diverse sizes are also of key importance in complex cases of large annuli and complex anatomy. Further long-term studies are needed to consolidate these results. Its introduction signifies a significant advancement in cardiology, aiming to enhance patient outcomes and quality of life. In the present review, we provide an update on new-generation Myval THV series and review the available clinical data published to date with an emphasis on diverse use in specific clinical scenarios.
The Effect of Micro-Computed Tomography Thresholding Methods on Bone Micromorphometric Analysis
Authors: Buyuksungur Arda , Szabó Bence Tamás , Dobai Adrienn , Orhan Kaan
Published: November 2024

Bone micromorphometric parameters are generally analyzed with micro CT to reveal two- and three-dimensional structures. These parameters are generally used for new bone formation studies such as tissue engineering and biomaterials studies. Different threshold methods are used for the image segmentation of bone micromorphometric parameters. However, these different threshold methods provide different results for the bones analyzed. This study aimed to compare thresholding methods to evaluate bone micromorphometric parameters in the mouse bone. A dataset containing 15 mouse tibia was used to analyze the different thresholding methods for bone micromorphometric parameter analysis. These threshold methods were used to analyze the mouse tibia (n = 15) with thresholded bones. The threshold methods and the analysis were used directly from CTAn (Bruker Micro-CT). The results were compared between the threshold methods, which included bone volume, trabecular number, connectivity, trabecular separation, and other parameters. There was agreement to some extent for all bone micromorphometric analyses using the different thresholding methods. The results showed that the thresholding method showed good agreement for connectivity and trabecular thickness, but the other parameters showed limited agreement. The evaluation of threshold methods allows for the comparison of image segmentation and the quantification of mouse tibia micromorphometric parameters. This study may enable the analysis of bone micromorphometric parameters using the relatively close threshold method in image segmentation across different research groups.
Spheroid-Exosome-Based Bioprinting Technology in Regenerative Medicine
Authors: Lee Hwa-Yong , Lee Jin Woo
Published: November 2024

Since the discovery that exosomes can exchange genes, their potential use as tools for tissue regeneration, disease diagnosis, and therapeutic applications has drawn significant attention. Emerging three-dimensional (3D) printing technologies, such as bioprinting, which allows the printing of cells, proteins, DNA, and other biological materials, have demonstrated the potential to create complex body tissues or personalized 3D models. The use of 3D spheroids in bioprinting facilitates volumetric tissue reconstruction and accelerates tissue regeneration via exosome secretion. In this review, we discussed a convergence approach between two promising technologies for bioprinting and exosomes in regenerative medicine. Among the various 3D cell culture methods used for exosome production, we focused on spheroids, which are suitable for mass production by bioprinting. We then summarized the research results on cases of bioprinting applications using the spheroids and exosomes produced. If a large number of spheroids can be supplied through bioprinting, the spheroid-exosome-based bioprinting technology will provide new possibilities for application in tissue regeneration, disease diagnosis, and treatment.
Preclinical Implementation of matRadiomics: A Case Study for Early Malformation Prediction in Zebrafish Model
Authors: Bini Fabiano , Missori Elisa , Pucci Gaia , Pasini Giovanni , Marinozzi Franco , Forte Giusi Irma , Russo Giorgio , Stefano Alessandro
Published: November 2024

Radiomics provides a structured approach to support clinical decision-making through key steps; however, users often face difficulties when switching between various software platforms to complete the workflow. To streamline this process, matRadiomics integrates the entire radiomics workflow within a single platform. This study extends matRadiomics to preclinical settings and validates it through a case study focused on early malformation differentiation in a zebrafish model. The proposed plugin incorporates Pyradiomics and streamlines feature extraction, selection, and classification using machine learning models (linear discriminant analysis—LDA; k-nearest neighbors—KNNs; and support vector machines—SVMs) with k-fold cross-validation for model validation. Classifier performances are evaluated using area under the ROC curve (AUC) and accuracy. The case study indicated the criticality of the long time required to extract features from preclinical images, generally of higher resolution than clinical images. To address this, a feature analysis was conducted to optimize settings, reducing extraction time while maintaining similarity to the original features. As a result, SVM exhibited the best performance for early malformation differentiation in zebrafish (AUC = 0.723; accuracy of 0.72). This case study underscores the plugin’s versatility and effectiveness in early biological outcome prediction, emphasizing its applicability across biomedical research fields.
Anatomical Characteristics of Cervicomedullary Compression on MRI Scans in Children with Achondroplasia
Authors: Trautwein Isabella , Behme Daniel , Kunkel Philip , Gerdes Jasper , Mohnike Klaus
Published: November 2024

This retrospective study assessed anatomical characteristics of cervicomedullary compression in children with achondroplasia. Twelve anatomical parameters were analyzed (foramen magnum diameter and area; myelon area; clivus length; tentorium and occipital angles; brainstem volume outside the posterior fossa; and posterior fossa, cerebellum, supratentorial ventricular system, intracranial cerebrospinal fluid, and fourth ventricle volumes) from sagittal and transversal T1- and T2-weighted magnetic resonance imaging (MRI) scans from 37 children with achondroplasia aged ≤ 4 years (median [range] 0.8 [0.1–3.6] years) and compared with scans from 37 children without achondroplasia (median age 1.5 [0–3.9] years). Mann–Whitney U testing was used for between-group comparisons. Foramen magnum diameter and area were significantly smaller in children with achondroplasia compared with the reference group (mean 10.0 vs. 16.1 mm [p < 0.001] and 109.0 vs. 160.8 mm2 [p = 0.005], respectively). The tentorial angle was also steeper in children with achondroplasia (mean 47.6 vs. 38.1 degrees; p < 0.001), while the clivus was significantly shorter (mean 23.5 vs. 30.3 mm; p < 0.001). Significant differences were also observed in myelon area, occipital angle, fourth ventricle, intracranial cerebrospinal fluid and supratentorial ventricular volumes, and the volume of brainstem protruding beyond the posterior fossa (all p < 0.05). MRI analysis of brain structures may provide a standardized value to indicate decompression surgery in children with achondroplasia.
A Comparison of the Microstructure and Mechanical Properties of RSW and RFSSW Joints in AA6061-T4 for Automotive Applications
Authors: Gale Damon , Smith Taylor , Hovanski Yuri , Namola Kate , Coyne Jeremy
Published: November 2024

To reduce vehicle weight and improve energy efficiency, automotive manufacturers are increasingly using aluminum body panels. However, the traditional joining method, Resistance Spot Welding (RSW), presents challenges like weld porosity and electrode degradation when used with aluminum. These issues have driven the industry to explore alternative, more effective methods for joining aluminum in vehicle manufacturing such as Refill Friction Stir Spot Welding (RFSSW). This research reports on a comparison of the microstructure and mechanical properties of welds made with RSW and RFSSW in AA6061-T4 automotive sheets. This comparison includes CT scanning, optical and SEM imaging, statistical microscopy, hardness testing, tensile testing, and fatigue testing. The results showed that RFSSW produced fully consolidated welds with a refined, equiaxed grain structure that outperformed RSW’s dendritic grain structure by as much as 73% in tensile testing and 2600% in fatigue testing. These results suggest that future designs utilizing RFSSW could incorporate fewer joints, reducing processing time, energy consumption, and tool wear. Cost studies also found that RFSSW consumes 2.5% of the energy that RSW does per joint, demonstrating that RFSSW is positioned as the preferred method for joining aluminum automotive sheets.
Research on Underwater Acoustic Target Recognition Based on a 3D Fusion Feature Joint Neural Network
Authors: Xu Weiting , Han Xingcheng , Zhao Yingliang , Wang Liming , Jia Caiqin , Feng Siqi , Han Junxuan , Zhang Li
Published: November 2024

In the context of a complex marine environment, extracting and recognizing underwater acoustic target features using ship-radiated noise present significant challenges. This paper proposes a novel deep neural network model for underwater target recognition, which integrates 3D Mel frequency cepstral coefficients (3D-MFCC) and 3D Mel features derived from ship audio signals as inputs. The model employs a serial architecture that combines a convolutional neural network (CNN) with a long short-term memory (LSTM) network. It replaces the traditional CNN with a multi-scale depthwise separable convolutional network (MSDC) and incorporates a multi-scale channel attention mechanism (MSCA). The experimental results demonstrate that the average recognition rate of this method reaches 87.52% on the DeepShip dataset and 97.32% on the ShipsEar dataset, indicating a strong classification performance.
Design Analysis Using Evaluation of Surf-Riding and Broaching by the IMO Second Generation Intact Stability Criteria for a Small Fishing Boat
Authors: Shin Dongmin , Moon Byungyoung
Published: November 2024

An evaluation was conducted to assess the surf-riding/broaching vulnerability of a 9.77-ton fishing boat by applying the regulations for stability assessment proposed by IMO (International Maritime Organization). Both Level 1 and 2 assessments were conducted and included a range of parameters along with the IMO second-generation intact stability criteria. In particular, it is considered three cases of wave forces acting on the hull for the surf-riding/broaching vulnerability Level 2 assessment calculations: (a) Froude-Krylov force (fFK) + 0.1M, (b) Froude-Krylov force (fFK) + added mass of the ship (Ma), and (c) Froude-Krylov force (fFK) + diffraction force (fD) + added mass of the ship (Ma). Previous results provided by IMO correspond to (b), and accurate calculation of wave forces helps to obtain more design margins. The design margins are high in the order (a) < (b) < (c), as described in the classification criteria. However, in certain cases, the assessment results may not differ significantly, so the hydrodynamic approximation assumption may be useful.
Isolation, Identification, and Analyzing the Biological Characteristics of Pathogens Causing Stem Rot of Lanzhou Onion During Postharvest Storage and Studying the Influence of Pathogen Infection on the Active Components of Lanzhou Onion
Authors: Wang Ruoxing , Zhang Hui , Zhang Qingru , Xi Jihui , Jiang Kunhao , Li Jinzhu , Xue Huali , Bi Yang
Published: November 2024

This study was conducted in order to explore the pathogens that cause stem rot of fresh onions during postharvest storage, identify the incidence of stem rot, investigate the influence of pathogen infection on the active components of onion, and provide a theoretical basis for disease control during the postharvest storage of fresh onions. The pathogens were isolated and purified from the junction between the rotten and healthy tissues of onion stem rot that occurred naturally during storage at room temperature by tissue separation; then, the pathogens were identified by morphological and molecular biological techniques, the biological characteristics of the pathogens were analyzed, and finally, the influence of pathogen infection on the active ingredients of onion was studied. The results suggested that the main pathogens causing stem rot of fresh onions during postharvest storage were Talaromyces pinophilus, Trichoderma simmonsii, and Talaromyces minioluteus. The optimum colony growth conditions for T. pinophilus were as follows: a temperature of 30 °C, a pH of 7, light for 24 h, maltose as a carbon source, and peptone as a nitrogen source; the lethal temperature was 65 °C for 15 min. For T. simmonsii, the lethal temperature was 60 °C for 15 min, and the optimum sporulation conditions were a temperature of 25 °C, a pH of 5–7, light for 24 h, a carbon source of sucrose, and a nitrogen source of yeast powder. For T. minioluteus, the lethal condition was 65 °C for 15 min; the optimum colony growth conditions were a temperature of 25 °C, a pH of 8–9, 24 h of darkness, a carbon source of maltose, and a nitrogen source of peptone. The relative content of sulfur compounds, as the active components of onions, was much lower in the infected onions than in the healthy onions due to infection by the pathogens T. pinophilus, T.simmonsii, and T.minioluteus. This study will provide a theoretical basis for further effective control of the occurrence of postharvest stem rot diseases of onions.
Material, Aerodynamic, and Operational Aspects of Single-Skin Paraglider
Authors: Maślanka Paulina , Korycki Ryszard
Published: November 2024

The operating comfort of a paraglider is created by the aerodynamic parameters as well as the mass and packing volume of the wing. A classic paraglider has upper and lower covers. To reduce the material and manufacturing costs as well as protect the environment, it is possible to introduce a single-skin wing. This article conducts an analysis of a single-skin paraglider covered only with upper panels, whereas the lower cover is applied only at the leading and trailing edges. The analysis is theoretically oriented; aerodynamic and structural calculations were performed using the ANSYS environment. The single-skin structure was evaluated in terms of the predicted behavior during flight and the material’s deformation under the influence of a specified pressure and the overloads acting on it. The results show that developing these structures may influence the creation of models with comparable aerodynamic characteristics to traditional ones. Additionally, the reduced masses and packing volumes of difficult-to-degrade materials are strongly correlated with saving costs and an ecological approach. No corresponding studies were found in the available literature. Thus, this presented analysis may result in a greater understanding and application of this paraglider type.
Polylactide Composites Reinforced with Pre-Impregnated Natural Fibre and Continuous Cellulose Yarns for 3D Printing Applications
Authors: Priya Muthe Lakshmi , Pickering Kim , Gauss Christian
Published: November 2024

Achieving high-performance 3D printing composite filaments requires addressing challenges related to fibre wetting and uniform fibre/polymer distribution. This study evaluates the effectiveness of solution (solvent-based) and emulsion (water-based) impregnation techniques to enhance fibre wetting in bleached flax yarns by polylactide (PLA). For the first time, continuous viscose yarn composites were also produced using both impregnation techniques. All the composites were carefully characterised throughout each stage of production. Initially, single yarns were impregnated and consolidated to optimise formulations and processing parameters. Solution impregnation resulted in the highest tensile strength (356 MPa) for PLA/bleached flax filaments, while emulsion impregnation yielded the highest tensile strength for PLA/viscose filaments (255 MPa) due to better fibre wetting and fibre distribution. Impregnated single yarns were then combined, with additional polymer added to produce filaments compatible with standard material extrusion 3D printers. Despite a reduction in the mechanical performance of the 3D-printed composites due to additional polymer impregnation, relatively high tensile and bending strengths were achieved, and the Charpy impact strength (>127 kJ/m2) for the viscose-based composite exceeded the reported values for bio-derived fibre reinforced composites. The robust mechanical performance of these filaments offers new opportunities for the large-scale additive manufacturing of structural components from bio-derived and renewable resources.
Destructive Effects of Slag from Municipal Waste Incineration Plants on Cement Composites
Authors: Sybis Marta , Mądrawski Jacek , Kostrzewski Wojciech , Konował Emilia , Walczak Zbigniew , Laks Ireneusz
Published: November 2024

The increasing production of solid waste and the scarcity of natural aggregates as a matter of fact have made waste recycling a necessity. One such waste, which is generated in large quantities, is slag. However, slag from incineration plants may contain harmful elements that adversely affect the physical, chemical and mechanical properties of cement composites. This study presents laboratory research results on the effect of slag from the Poznan Municipal Waste Thermal Conversion Plant (Poland) on the physicochemical properties of cement composites. The samples were analyzed by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). It was shown that the slag analyzed contained significant amounts of aluminum, which had a direct effect on the structure of the concrete. An example of this influence is the release of hydrogen during reactions, which causes swelling and cracking of the concrete and reduces its mechanical strength. The authors emphasize that waste aggregate (slag) can be effectively used in the production of concrete after appropriate processing that reduces the risk of adverse effects.
Hybrid High-Power AlGaN/CdZnO/GaN/AlGaN HEMT with High Breakdown Voltage
Authors: Kim Bonghwan , Park Seung-Hwan
Published: November 2024

This study investigates the effects of incorporating a CdZnO layer in place of the conventional InGaN layer in an AlGaN/InGaN/GaN/AlGaN/SiC high-electron mobility transistor (HEMT) structure. We examine the resulting characteristics and assess the potential of high-power HEMT applications, including high-power switching converters, through simulation analysis. Both structures demonstrate increased drain current and transconductance with increasing Al content in the barrier layer. However, HEMTs with a CdZnO layer exhibit higher drain current compared to those with an InGaN layer at the same Al content. The breakdown voltage decreases rapidly with increasing Al content, attributed to changes in electric field distribution. HEMTs with a CdZnO/GaN channel exhibit a slightly higher breakdown voltage (~795 V) compared to those with an InGaN/GaN channel (~768 V) at a lower Al content of x = 0.10. These results suggest that CdZnO-based HEMTs have significant potential for high-power, high-frequency applications.
Pulsed Eddy Current Imaging of Partially Missing Solder in Brazing Joints of Stainless Steel Core Plates
Authors: Zhu Changchun , Chen Hanqing , Zhu Xuecheng , Zeng Hui , Xu Zhiyuan
Published: November 2024

Stainless steel core plates (SSCPs) show great potential for modular construction due to their superiority of excellent mechanical properties, light weight, and low cost over traditional concrete and honeycomb structures. During the brazing process of SSCP joints which connect the skin panel and core tubes, it is difficult to keep an even heat flow of inert gas in the vast furnace, which can lead to partially missing solder defects in brazing joints. Pulsed eddy current imaging (PECI) has demonstrated feasibility for detecting missing solder defects, but various factors including lift-off variation and image blurring can deteriorate the quality of C-scan images, resulting in inaccurate evaluation of the actual state of the brazed joints. In this study, a differential pulsed eddy current testing (PECT) probe is designed to reduce the lift-off noise of PECT signals, and a mask-based image segmentation and thinning method is proposed to eliminate the blurring effect of C-scan images. The structure of the designed probe was optimized based on finite element simulation and the positive peak of the PECT signal was selected as the signal feature. Experiments with the aid of a scanning device are then carried out to image the interrogated regions of the SSCP specimen. The peak values of the signals were collected in a matrix to generate images of the scanned brazing joints. Results show that lift-off noise is significantly reduced by using the differential probe. Image blurring caused by the convolution effect of the probe’s point spread function with the imaging object was eliminated using a mask-based image segmentation and thinning method. The restored C-scan images enhance the sharpness of the profiles of the brazing joints and the opening in the images accurately reflect the missing solder of the brazed joints.
Study on Surface Quality Analysis of an Uncoated Boron Steel and Its Oxide Layer Suppression Method for Hot Stamping
Authors: Lee Jiho , Song Junghan , Bae Gihyun
Published: November 2024

This study investigates the effects of hot stamping on boron steel surface properties, comparing uncoated steel to Al–Si-coated steel, with a focus on developing atmosphere-controlled hot stamping technology. Experiments using a hat-shaped specimen revealed that uncoated steel formed a thick oxide layer due to exposure to atmospheric oxygen at high temperatures, negatively impacting surface quality and weldability. In contrast, the Al–Si-coated steel showed no oxide formation. Although uncoated steel exhibited higher average Vickers hardness, the detrimental effects of the oxide layer on weld quality necessitate advancements in process technology. A lab-scale hot stamping simulator was developed to control atmospheric oxygen levels, utilizing a donut-shaped induction heating coil to heat the material above 1000 °C, followed by rapid cooling in a forming die. Results demonstrated that maintaining oxygen concentrations below 6% significantly reduced oxide layer thickness, with near-vacuum conditions eliminating oxide formation altogether. These findings emphasize the critical role of oxygen control in enhancing the surface quality and weldability of uncoated boron steel for ultra-high-strength automotive applications, potentially reducing manufacturing costs while ensuring part performance.
Machining Characteristics During Short Hole Drilling of Titanium Alloy Ti10V2Fe3Al
Authors: Storchak Michael
Published: November 2024

The single-phase titanium ß-alloy Ti10V2Fe3Al (Ti-1023) has been widely used in the aerospace industry due to its unique mechanical properties, which include high fatigue strength and fracture toughness, as well as high corrosion resistance. On the other hand, these unique properties significantly hinder the cutting processes of this material, especially those characterized by a closed machining process area, such as drilling. This paper is devoted to the study of the short hole drilling process of the above-mentioned titanium alloy using direct measurements and numerical modeling. Measurements of the cutting force components in the drilling process and determination of the resultant cutting force and total cutting power were performed. The macro- and microstructure of chips generated during drilling were analyzed, and the dependence of the chip compression ratio and the distance between neighboring segments of serrated chips on cutting speed and drill feed was determined. Experimental studies were supplemented by determining the temperature on the lateral clearance face of the drill’s outer cutting insert in dependence on the cutting modes. For the modeling of the drilling process using the finite element model, the parameters of the triad of component submodels of the numerical model were determined: the machined material model, the model of contact interaction between the tool and the machined material, and the fracture model of the machined material. The determination of these parameters was performed through the DOE sensitivity analysis. The target values for performing this analysis were the total cutting power and the distance between neighboring chip segments. The maximum deviation between the simulated and experimentally determined values of the resulting cutting force is no more than 25%. At the same time, the maximum deviation between the measured values of the temperature on the lateral clearance face of the drill’s outer cutting insert and the corresponding simulated values is 26.1%.