bluuebunny/crossref_metadata_2025 huggingface dataset:
Field Evaluations of Sulfuryl Fluoride Fumigation for Control of the Common Bed Bug (Hemiptera: Cimicidae), Using a 1.9× Dosage Factor in Motor Vehicles and Filled Cargo Trailers
Authors: Todd D B , Miller D M , Gordon J R
Published: February 2021

This study investigated the efficacy of using Vikane gas fumigant (sulfuryl fluoride) at the 1.9× dosage rate for eliminating bed bugs (Cimex lectularius L.) in two challenging infestation situations: personal vehicles, and confined spaces densely packed with personal belongings. The vehicles used in this study were large minivans with seating that folded into the floor. The confined spaces were cargo trailers filled to 85% capacity with books, furniture, and other household items. Each van and trailer was equipped with ~90 sentinel bed bugs consisting of three groups of 9–11 bed bug eggs, 10 nymphs, and 10 adults. The Vikane Fumiguide calculator was used to determine the target dosage (g-h/m3) to apply in each replicate (e.g., one van or trailer). Sulfuryl fluoride concentrations were measured throughout the fumigation process using a Spectros SF-ReportIR. Concentration readings were input into the Fumiguide to determine when the accumulated dosage (g-h/m3) was achieved, and when aeration should be initiated. After aeration was complete, the sentinel bed bugs were removed from the replicates and bed bug nymph and adult mortality was recorded. Bed bug eggs were monitored for 23 d to determine latent mortality. Fumigated bed bug mortality for each replication was 100% regardless of life stage. Latent mortality was observed in a single bed bug egg, but the first instar never fully eclosed. This study determined that fumigation with sulfuryl fluoride at the 1.9× dosage factor is an effective method for eliminating resistant bed bugs from vehicles and personal belongings in densely packed situations.
Comparison of Trap Collections and Cost of Commercially Available and Homemade Yellowjacket (Hymenoptera: Vespidae) Traps in Lake County, California
Authors: Urquhart Cassandra , Scott Jamesina J
Published: February 2021

Yellowjackets are notable pests of humans due to their opportunistic foraging behaviors, painful stings, and potential for causing dangerous allergic reactions. Baited traps provide a useful supplement for controlling yellowjackets compared with nest treatments, which are often dangerous, time consuming, costly, and do little to prevent nuisance interactions between humans and foragers. This study compares three homemade yellowjacket traps and three commercially available traps in Lake County, California, to determine efficacy and cost benefit. Traps were set at five sites and randomly rotated between six plots per site and baits were changed every 2 wk per commercial manufacturer recommendations. Cost benefit was determined using material and bait cost, as well as bait change frequency and overall trap efficacy. Yellowjacket count data were analyzed using a hurdle model. Traps compared included the Rescue! Yellowjacket trap, the Rescue! Wasp, Hornet, and Yellowjacket trap, the Victor Yellowjacket trap, a homemade bottle trap, jar trap, and homemade jug trap. The total number of yellowjackets collected was 33,321. The trap that collected the highest number of yellowjackets was the Rescue! Yellowjacket trap (n = 19,257) and the trap that collected the fewest yellowjackets was the jar trap (n = 65). The Rescue! Yellowjacket trap was the most cost-effective, calculated at approximately $0.40/100 yellowjackets collected. The jar trap was the least cost-effective, calculated at approximately $31.10/100 yellowjackets collected. The Rescue! Yellowjacket trap was overall the most effective and cost-effective trap evaluated for Lake County, California.
Host Suitability Index for Polyphagous Tephritid Fruit Flies
Authors: Follett Peter A , Haynes Fay E M , Dominiak Bernard C
Published: February 2021

Tephritid fruit flies are major economic pests for fruit production and are an impediment to international trade. Different host fruits are known to vary in their suitability for fruit flies to complete their life cycle. Currently, international regulatory standards that define the likely legal host status for tephritid fruit flies categorize fruits as a natural host, a conditional host, or a nonhost. For those fruits that are natural or conditional hosts, infestation rate can vary as a spectrum ranging from highly attractive fruits supporting large numbers of fruit flies to very poor hosts supporting low numbers. Here, we propose a Host Suitability Index (HSI), which divides the host status of natural and conditional hosts into five categories based on the log infestation rate (number of flies per kilogram of fruit) ranging from very poor (<0.1), poor (0.1–1.0), moderately good (1.0–10.0), good (10–100), and very good (>100). Infestation rates may be determined by field sampling or cage infestation studies. We illustrate the concept of this index using 21 papers that examine the host status of fruits in five species of polyphagous fruit flies in the Pacific region: Bactrocera tryoni Froggatt, Bactrocera dorsalis (Hendel), Bactrocera latifrons (Hendel), Zeugodacus cucurbitae (Coquillett), and Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). This general-purpose index may be useful in developing systems approaches that rely on poor host status, for determining surveillance and detection protocols for potential incursions, and to guide the appropriate regulatory response during fruit fly outbreaks.
Control of Pierce’s Disease Through Areawide Management of Glassy-Winged Sharpshooter (Hemiptera: Cicadellidae) and Roguing of Infected Grapevines
Authors: Haviland David R , Stone-Smith Beth , Gonzalez Minerva
Published: February 2021

The General Beale Pilot Project serves as a case study for the use of areawide monitoring and treatment programs for glassy-winged sharpshooter (GWSS), Homalodisca vitripennis (Germar), and monitoring and roguing programs for grapevines infected with Xylella fastidiosa, to achieve regional management of Pierce’s disease. The Project is located in southeast Kern County, CA, and contains ~2,800 ha of citrus and grapevines grown within approximately 50 km2. For nearly 20 yr, an average of 470 traps have been used to monitor GWSS populations regionally by the California Department of Food and Agriculture, and to inform coordinated, areawide treatments by the USDA-APHIS Areawide Treatment Program to overwintering GWSS in citrus. Grape growers were responsible for treating their own vineyards, and for the roguing of infected grapevines based on surveys provided by the University of California. Herein, we provide a history of the General Beale Pilot Project, broken down into six eras based on levels of Project success, which incorporate data on GWSS captures, pesticide use, and disease incidence. We describe patterns of success related to the regional coordination of effective treatment and roguing programs that can be used by grape and neighboring citrus growers for areawide management of Pierce’s disease. We conclude by describing current and future challenges for Pierce’s disease management, including pesticide availability and resistance, GWSS refuges, the inability to detect and rogue infected vines in the year they become infected, and the sustainability of voluntary programs that rely on public funding.
Gifts of an enemy: scavenging dynamics in the presence of wolves (Canis lupus)
Authors: Klauder Kaija J , Borg Bridget L , Sivy Kelly J , Prugh Laura R
Published: February 2021

Carrion represents an important resource for carnivores. Examining competition for carrion in a risk–reward framework allows for a better understanding of how predator guilds compete for and benefit from carrion. We used trail camera data to compare wintertime carrion use and vigilance behavior of four carnivores in Denali National Park and Preserve. We found that carrion use was dominated by wolves (Canis lupus) and wolverines (Gulo gulo), followed by red foxes (Vulpes vulpes) and coyotes (Canis latrans). Wolves and wolverines were twice as likely to visit a carcass as foxes and coyotes, and their visits were longer and more numerous. Our results suggest scavenging animals reduced their risk exposure primarily by reducing their use of carrion, with some evidence of increased vigilance at busy sites. We found that carrion use and behavior at carcass sites were influenced by the mortality type of the carcass, the age of the carcass, and the long-term intensity of wolf use in the area. Our results also suggest that wolves are the “top scavenger,” and indicate that intraguild competition for carrion strongly affects which species benefit from carrion, with larger and more aggressive species dominating.
Kangaroos at maximum capacity: health assessment of free-ranging eastern grey kangaroos on a coastal headland
Authors: Brandimarti Maquel E , Gray Rachael , Silva Fabiola R O , Herbert Catherine A
Published: February 2021

Sprawling urban development is fragmenting the landscape and native wildlife habitats on the Australian east coast. The impact of this rapid urbanization on wildlife health is largely unknown. This study surveyed the health of a high-density (5.4 individuals per ha) population of eastern grey kangaroos (Macropus giganteus) affected by urban encroachment and prolonged drought. Blood parameters (hematological and serum protein), trace element and heavy metal concentrations, and parasite counts (fecal worm egg counts, ticks, and mites) are reported for a sample of ≤ 54 kangaroos at Look at Me Now Headland, New South Wales, Australia. These parameters were compared to lower density kangaroo populations from other sites in New South Wales. We found the health and welfare of this population to be severely compromised, with nonregenerative anemia and nutritional deficiencies evident. Our results indicate that high-density kangaroo populations isolated by urban encroachment are at significant health risk. To prevent further decline in this population’s health, we discuss management strategies that could be employed, concurrent with ongoing health and disease monitoring, to mitigate the poor health outcomes in this population. We conclude that it is essential to retain habitat connectivity when altering land use in areas with resident kangaroo populations if managers are to maintain healthy populations.
Fasudil alleviated insulin resistance through promotion of proliferation, attenuation of cell apoptosis and inflammation and regulation of RhoA/Rho kinase/insulin/nuclear factor-κB signalling pathway in HTR-8/SVneo cells
Authors: Bai Yu , Du Qiang , Zhang Le , Li Ling , Tang Lei , Zhang Wei , Du Runyu , Li Ping , Li Ling
Published: February 2021

Objectives

The aim of this study was to evaluate the effects of fasudil on insulin resistance (IR) in HTR-8/SVneo cells.

Methods

HTR-8/SVneo cells were treated with insulin or/and fasudil. Cell proliferation, apoptosis, inflammation and related signalling pathways were assessed.

Key findings

Insulin treatment significantly enhanced the protein expressions of RhoA and Rho kinase (ROCK1 and ROCK2), but decreased glucose consumption. Administration of fasudil effectively promoted glucose uptake. Moreover, fasudil enhanced cell viability and the level of proliferating cell nuclear antigen (PCNA). Insulin-mediated cell apoptosis was inhibited by fasudil via the down-regulation of bax and cleaved-caspase-3, and the up-regulation of bcl-2. At the same time, fasudil led to the reduction of IL-1β, TNF-α, IL-6 and IL-8 mRNA levels in insulin-treated cells. In addition, RhoA, ROCK2 and phosphorylated myosin phosphatase target subunit-1 (p-MYPT-1) expressions were down-regulated by fasudil. Importantly, fasudil activated insulin receptor substrate-1 (IRS-1) through increasing p-IRS-1 (Tyr612) and p-Akt expressions. The nuclear NF-κB p65 and p-IκB-α levels were reduced via the administration of fasudil in insulin-treated cells.

Conclusions

Fasudil mitigated IR by the promotion of cell proliferation, inhibition of apoptosis and inflammation and regulation of RhoA/ROCK/insulin/NF-κB signalling pathway through in vitro studies.
The trafficking machinery of lytic and protein storage vacuoles: how much is shared and how much is distinct?
Authors: Zhang Xiuxiu , Li Hui , Lu Hai , Hwang Inhwan
Published: February 2021

Plant cells contain two types of vacuoles, the lytic vacuole (LV) and protein storage vacuole (PSV). LVs are present in vegetative cells, whereas PSVs are found in seed cells. The physiological functions of the two types of vacuole differ. Newly synthesized proteins must be transported to these vacuoles via protein trafficking through the endomembrane system for them to function. Recently, significant advances have been made in elucidating the molecular mechanisms of protein trafficking to these organelles. Despite these advances, the relationship between the trafficking mechanisms to the LV and PSV remains unclear. Some aspects of the trafficking mechanisms are common to both types of vacuole, but certain aspects are specific to trafficking to either the LV or PSV. In this review, we summarize recent findings on the components involved in protein trafficking to both the LV and PSV and compare them to examine the extent of overlap in the trafficking mechanisms. In addition, we discuss the interconnection between the LV and PSV provided by the protein trafficking machinery and the implications for the identity of these organelles.
Broad-band study of OQ 334 during its flaring state
Authors: Prince Raj , Khatoon Rukaiya , Stalin C S
Published: February 2021

The blazar OQ 334 displayed a γ-ray flare in 2018, after being in the long quiescent γ-ray state since 2008. Subsequent to the flare, the source was in a higher γ-ray flux state and again flared in 2020. We present here the first spectral and timing analysis of the source at its various flaring states. During the higher γ-ray state, we found four major peaks identified as P1, P2, P3, and P4. From timing analysis we found rise and decay time of the order of hours with the fastest variability time of 9.01 ± 0.78 h. We found the highest γ-ray photon of 77 GeV during P4, which suggests the location of the γ-ray emitting region at the outer edge of the broad line region or the inner edge of the torus. The γ-ray spectral analysis of the source indicates that during P4, the γ-ray spectrum clearly deviates from the power-law behaviour. From cross-correlation analysis of the γ-ray and radio light curves, we found that the two emission regions are separated by about 11 pc. Our broad-band spectral energy distribution modelling of the source during quiescent and active phases indicates that more electron and proton power are required to change the source from low flux to high flux state. The Anderson–Darling test and histogram fitting results suggest that the three days binned γ-ray fluxes follow a lognormal distribution.
The relativistic binary programme on MeerKAT: science objectives and first results
Authors: Kramer M , Stairs I H , Venkatraman Krishnan V , Freire P C C , Abbate F , Bailes M , Burgay M , Buchner S , Champion D J , Cognard I , Gautam T , Geyer M , Guillemot L , Hu H , Janssen G , Lower M E , Parthasarathy A , Possenti A , Ransom S , Reardon D J , Ridolfi A , Serylak M , Shannon R M , Spiewak R , Theureau G , van Straten W , Wex N , Oswald L S , Posselt B , Sobey C , Barr E D , Camilo F , Hugo B , Jameson A , Johnston S , Karastergiou A , Keith M , Osłowski S
Published: February 2021

We describe the ongoing Relativistic Binary programme (RelBin), a part of the MeerTime large survey project with the MeerKAT radio telescope. RelBin is primarily focused on observations of relativistic effects in binary pulsars to enable measurements of neutron star masses and tests of theories of gravity. We selected 25 pulsars as an initial high priority list of targets based on their characteristics and observational history with other telescopes. In this paper, we provide an outline of the programme, and present polarization calibrated pulse profiles for all selected pulsars as a reference catalogue along with updated dispersion measures. We report Faraday rotation measures for 24 pulsars, twelve of which have been measured for the first time. More than a third of our selected pulsars show a flat position angle swing confirming earlier observations. We demonstrate the ability of the Rotating Vector Model, fitted here to seven binary pulsars, including the Double Pulsar (PSR J0737–3039A), to obtain information about the orbital inclination angle. We present a high time resolution light curve of the eclipse of PSR J0737–3039A by the companion’s magnetosphere, a high-phase-resolution position angle swing for PSR J1141–6545, an improved detection of the Shapiro delay of PSR J1811–2405, and pulse scattering measurements for PSRs J1227–6208, J1757–1854, and J1811–1736. Finally, we demonstrate that timing observations with MeerKAT improve on existing data sets by a factor of, typically, 2–3, sometimes by an order of magnitude.
The excess of cool supergiants from contemporary stellar evolution models defies the metallicity-independent Humphreys–Davidson limit
Authors: Gilkis Avishai , Shenar Tomer , Ramachandran Varsha , Jermyn Adam S , Mahy Laurent , Oskinova Lidia M , Arcavi Iair , Sana Hugues
Published: February 2021

The Humphreys–Davidson (HD) limit empirically defines a region of high luminosities (log10(L/L⊙) ≳ 5.5) and low effective temperatures ($T_{\rm eff} \lesssim 20 \, {\rm kK}$) on the Hertzsprung–Russell diagram in which hardly any supergiant stars are observed. Attempts to explain this limit through instabilities arising in near- or super-Eddington winds have been largely unsuccessful. Using modern stellar evolution, we aim to re-examine the HD limit, investigating the impact of enhanced mixing on massive stars. We construct grids of stellar evolution models appropriate for the Small Magellanic Cloud (SMC) and Large Magellanic Cloud (LMC), as well as for the Galaxy, spanning various initial rotation rates and convective overshooting parameters. Significantly enhanced mixing apparently steers stellar evolution tracks away from the region of the HD limit. To quantify the excess of overluminous stars in stellar evolution simulations, we generate synthetic populations of massive stars and make detailed comparisons with catalogues of cool ($T_\mathrm{eff} \le 12.5\, \mathrm{kK}$) and luminous (log10(L/L⊙) ≥ 4.7) stars in the SMC and LMC. We find that adjustments to the mixing parameters can lead to agreement between the observed and simulated red supergiant populations, but for hotter supergiants the simulations always overpredict the number of very luminous (log10(L/L⊙) ≥ 5.4) stars compared to observations. The excess of luminous supergiants decreases for enhanced mixing, possibly hinting at an important role mixing has in explaining the HD limit. Still, the HD limit remains unexplained for hotter supergiants.
High-contrast and resolution near-infrared photometry of the core of R136
Authors: Khorrami Zeinab , Langlois Maud , Clark Paul C , Vakili Farrokh , Buckner Anne S M , Gonzalez Marta , Crowther Paul , Wünsch Richard , Palouš Jan , Lumsden Stuart , Moraux Estelle
Published: February 2021

We present the sharpest and deepest near-infrared photometric analysis of the core of R136, a newly formed massive star cluster at the centre of the 30 Doradus star-forming region in the Large Magellanic Cloud. We used the extreme adaptive optics of the SPHERE focal instrument implemented on the ESO Very Large Telescope and operated in its IRDIS imaging mode for the second time with longer exposure time in the H and K filters. Our aim was to (i) increase the number of resolved sources in the core of R136, and (ii) to compare with the first epoch to classify the properties of the detected common sources between the two epochs. Within the field of view (FOV) of 10.8″ × 12.1″ ($2.7\,\text {pc}\times 3.0\, \text {pc}$), we detected 1499 sources in both H and K filters, for which 76 per cent of these sources have visual companions closer than 0.2″. The larger number of detected sources enabled us to better sample the mass function (MF). The MF slopes are estimated at ages of 1, 1.5, and 2 Myr, at different radii, and for different mass ranges. The MF slopes for the mass range of 10–300 M⊙ are about 0.3 dex steeper than the mass range of 3–300 M⊙, for the whole FOV and different radii. Comparing the JHK colours of 790 sources common in between the two epochs, 67 per cent of detected sources in the outer region (r > 3″) are not consistent with evolutionary models at 1–2 Myr and with extinctions similar to the average cluster value, suggesting an origin from ongoing star formation within 30 Doradus, unrelated to R136.
Evidence that short-period AM CVn systems are diverse in outburst behaviour
Authors: Duffy C , Ramsay G , Steeghs D , Dhillon V , Kennedy M R , Mata Sánchez D , Ackley K , Dyer M , Lyman J , Ulaczyk K , Galloway D K , O’Brien P , Noysena K , Nuttall L , Pollacco D
Published: February 2021

We present results of our analysis of up to 15 yr of photometric data from eight AM CVn systems with orbital periods between 22.5 and 26.8 min. Our data have been collected from the GOTO, ZTF, Pan-STARRS, ASAS-SN, and Catalina all-sky surveys and amateur observations collated by the AAVSO. We find evidence that these interacting ultracompact binaries show a similar diversity of long-term optical properties as the hydrogen accreting dwarf novae. We found that AM CVn systems in the previously identified accretion disc instability region are not a homogenous group. Various members of the analysed sample exhibit behaviour reminiscent of Z Cam systems with long superoutbursts (SOs) and standstills, SU UMa systems with regular, shorter SOs, and nova-like systems that appear only in a high state. The addition of TESS full frame images of one of these systems, KL Dra, reveals the first evidence for normal outbursts appearing as a precursor to SOs in an AM CVn system. Our results will inform theoretical modelling of the outbursts of hydrogen deficient systems.
Density profile evolution during prestellar core collapse: collapse starts at the large scale
Authors: Gómez Gilberto C , Vázquez-Semadeni Enrique , Palau Aina
Published: February 2021

We study the gravitationally dominated, accretion-driven evolution of a prestellar core. In our model, as the core’s density increases, it remains immersed in a constant-density environment and so it accretes from this environment, increasing its mass and reducing its Jeans length. Assuming a power-law density profile ρ ∝ r−p, we compute the rate of change of the slope p, and show that the value p = 2 is stationary, and furthermore, an attractor. The radial profile of the Jeans length scales as rp/2, implying that, for p < 2, there is a radius below which the region is smaller than its Jeans length, thus appearing gravitationally stable and in need of pressure confinement, while, in reality, it is part of a larger scale collapse and is undergoing compression by the infalling material. In this region, the infall speed decreases towards the centre, eventually becoming subsonic, thus appearing ‘coherent’, without the need for turbulence dissipation. We present a compilation of observational determinations of density profiles in dense cores and show that the distribution of their slopes peaks at p ∼ 1.7–1.9, supporting the notion that the profile steepens over time. Finally, we discuss the case of magnetic support in a core in which the field scales as B ∝ ρβ. For the expected value of β = 2/3, this implies that the mass to magnetic flux ratio also decreases towards the central parts of the cores, making them appear magnetically supported, while, in reality, they may be part of larger collapsing supercritical region. We conclude that local signatures of either thermal or magnetic support are not conclusive evidence of stability, that the gravitational instability of a region must be established at the large scales, and that the prestellar stage of collapse is dynamic rather than quasi-static.
A finite volume method for two-moment cosmic ray hydrodynamics on a moving mesh
Authors: Thomas T , Pfrommer C , Pakmor R
Published: February 2021

We present a new numerical algorithm to solve the recently derived equations of two-moment cosmic ray hydrodynamics (CRHD). The algorithm is implemented as a module in the moving mesh Arepo code. Therein, the anisotropic transport of cosmic rays (CRs) along magnetic field lines is discretized using a path-conservative finite volume method on the unstructured time-dependent Voronoi mesh of Arepo. The interaction of CRs and gyroresonant Alfvén waves is described by short time-scale source terms in the CRHD equations. We employ a custom-made semi-implicit adaptive time stepping source term integrator to accurately integrate this interaction on the small light-crossing time of the anisotropic transport step. Both the transport and the source term integration step are separated from the evolution of the magnetohydrodynamical equations using an operator split approach. The new algorithm is tested with a variety of test problems, including shock tubes, a perpendicular magnetized discontinuity, the hydrodynamic response to a CR overpressure, CR acceleration of a warm cloud, and a CR blast wave, which demonstrate that the coupling between CR and magnetohydrodynamics is robust and accurate. We demonstrate the numerical convergence of the presented scheme using new linear and non-linear analytic solutions.
Testing models of periodically modulated FRB activity
Authors: Katz J I
Published: February 2021

The activity of the repeating FRB 20180916B is periodically modulated with a period of 16.3 d, and FRB 121102 may be similarly modulated with a period of about 160 d. In some models of this modulation, the period derivative is insensitive to the uncertain parameters; these models can be tested by measurement of or bounds on the derivative. In other models, values of the uncertain parameters can be constrained. Periodic modulation of aperiodic bursting activity may result from emission by a narrow beam wandering within a cone or funnel along the axis of a precessing disc, such as the accretion discs in X-ray binaries. The production of FRB 200428 by a neutron star that is neither accreting nor in a binary then shows universality: coherent emission occurring in a wide range of circumstances.
Exploring the planetary-mass population in the Upper Scorpius association
Authors: Lodieu N , Hambly N C , Cross N J G
Published: February 2021

We aim at identifying very low-mass isolated planetary-mass member candidates in the nearest OB association to the Sun, Upper Scorpius (USco) (145 pc; 5–10 Myr), to constrain the form and shape of the luminosity function and mass spectrum in this regime. We conducted a deep multiband (Y = 21.2, J = 20.5, Z = 22.0 mag) photometric survey of 6 deg2 in the central region of USco. We extend the current sequence of astrometric and spectroscopic members by about two magnitudes in Y and one magnitude in J, reaching potentially T-type free-floating members in the association with predicted masses below 5 Jupiter masses, well into the planetary-mass regime. We extracted a sample of 57 candidates in this area and present infrared spectroscopy confirming two of them as young L-type members with characteristic spectral features of 10-Myr-old brown dwarfs. Among the 57 candidates, we highlight 10 new candidates fainter than the coolest members previously confirmed spectroscopically. We do not see any obvious sign of decrease in the mass spectrum of the association, suggesting that star processes can form substellar objects with masses down to 4–5 Jupiter masses.
Formation of supermassive black hole seeds in nuclear star clusters via gas accretion and runaway collisions
Authors: Das Arpan , Schleicher Dominik R G , Leigh Nathan W C , Boekholt Tjarda C N
Published: February 2021

More than 200 supermassive black holes (SMBHs) of masses $\gtrsim 10^9\, \mathrm{M_{\odot }}$ have been discovered at z ≳ 6. One promising pathway for the formation of SMBHs is through the collapse of supermassive stars (SMSs) with masses $\sim 10^{3}{-}10^{5} \, \mathrm{M_{\odot }}$ into seed black holes which could grow upto few times $10^9\, \mathrm{M_{\odot }}$ SMBHs observed at z ∼ 7. In this paper, we explore how SMSs with masses $\sim 10^{3}{-}10^{5} \, \mathrm{M_{\odot }}$ could be formed via gas accretion and runaway stellar collisions in high-redshift, metal-poor nuclear star clusters (NSCs) using idealized N-body simulations. We explore physically motivated accretion scenarios, e.g. Bondi–Hoyle–Lyttleton accretion and Eddington accretion, as well as simplified scenarios such as constant accretions. While gas is present, the accretion time-scale remains considerably shorter than the time-scale for collisions with the most massive object (MMO). However, overall the time-scale for collisions between any two stars in the cluster can become comparable or shorter than the accretion time-scale, hence collisions still play a crucial role in determining the final mass of the SMSs. We find that the problem is highly sensitive to the initial conditions and our assumed recipe for the accretion, due to the highly chaotic nature of the problem. The key variables that determine the mass growth mechanism are the mass of the MMO and the gas reservoir that is available for the accretion. Depending on different conditions, SMSs of masses $\sim 10^{3}{-}10^{5} \, \mathrm{M_{\odot }}$ can form for all three accretion scenarios considered in this work.
Maximum mass of hybrid star formed via shock-induced phase transition in cold neutron stars
Authors: Mallick Ritam , Singh Shailendra , Nandi Rana
Published: February 2021

This paper studies the maximum mass limit of the hybrid star formed after the shock-induced phase transition of a cold neutron star. By employing hadronic and quark equation of state that satisfies the current mass bound, we use combustion adiabat conditions to find such a limit. The combustion adiabat condition results in a local or a global maximum pressure at an intermediate density range. The maximum pressure corresponds to a local or global maximum mass for the phase transformed hybrid star. The phase transition is usually exothermic if we have a local maximum mass. The criteria for exothermic or endothermic phase transition depend on whether the quark pressure/energy ratios to nuclear pressure/energy are smaller or greater than 1. We find that exothermic phase transition in a cold neutron star usually results in hybrid stars whose mass is smaller than a parent neutron star. The phase transition is endothermic for a global maximum pressure; thereby, one gets a global maximum mass. Hybrid stars much massive than phase transformed local maximum mass can be formed, provided there is some external energy source during the phase transition process. However, for some cases, even massive hybrid stars can form with exothermic phase transition for equations of state having global maximum pressure.
The post-Herschelview of intrinsic AGN emission: constructing templates for galaxy and AGN emission at IR wavelengths
Authors: Bernhard E , Tadhunter C , Mullaney J R , Grimmett L P , Rosario D J , Alexander D M
Published: February 2021

Measuring the star-forming properties of active galactic nucleus (AGN) hosts is key to our understanding of galaxy formation and evolution. However, this topic remains debated, partly due to the difficulties in separating the infrared (i.e. 1–1000 ${\rm \mu m}$) emission into AGN and star-forming components. Taking advantage of archival far-infrared data from Herschel, we present a new set of AGN and galaxy infrared templates and introduce the spectral energy distribution fitting code iragnsep. Both can be used to measure infrared host galaxy properties, free of AGN contamination. To build these, we used a sample of 100 local (z < 0.3), low-to-high luminosity AGNs (i.e. Lbol$\ \sim \ 10^{42-46}$ erg s−1), selected from the 105-month Swift–BAT X-ray survey, which have archival Spitzer–IRS spectra and Herschel photometry. We first built a set of seven galaxy templates using a sample of 55 star-forming galaxies selected via infrared diagnostics. Using these templates, combined with a flexible model for the AGN contribution, we extracted the intrinsic infrared emission of our AGN sample. We further demonstrate that we can reduce the diversity in the intrinsic shapes of AGN spectral energy distributions down to a set of three AGN templates, of which two represent AGN continuum, and one represents silicate emission. Our results indicate that, on average, the contribution of AGNs to the far-infrared (λ ≳ 50 ${\rm \mu m}$) is not as high as suggested by some recent work. We further show that the need for two infrared AGN continuum templates could be related to nuclear obscuration, where one of our templates appears dominated by the emission of the extended polar dust.
Very low-frequency oscillations from the 11 Hz pulsar in Terzan 5: frame dragging back on the table.
Authors: du Buisson L , Motta S , Fender R
Published: February 2021

We present a re-analysis of 47 Rossi X-ray Timing Explorer observations of the 11 Hz accreting pulsar IGR J17480-2446 in Terzan 5 during its 2010 outburst. We studied the fast-time variability properties of the source and searched for quasi-periodic oscillations (QPOs) in a large-frequency range. General relativity predicts that frame dragging occurs in the vicinity of a spinning compact object and induces the precession of matter orbiting said object. The relativistic precession model predicts that this frame dragging can be observed as QPOs with a characteristic frequency in the light curves of accreting compact objects. Such QPOs have historically been classified as horizontal branch oscillations in neutron star (NS) systems, and for a NS spinning at 11 Hz, these oscillations are expected at frequencies below 1 Hz. However, previous studies of IGR J17480-2446 have classified QPOs at 35–50 Hz as horizontal branch oscillations, thus casting doubts on the frame-dragging nature of such QPOs. Here, we report the detection of seven very low-frequency QPOs, previously undetected, with centroid frequencies below 0.3 Hz, and which can be ascribed to frame dragging. We also discuss the possible nature of the QPOs detected at 35–50 Hz in this alternative scenario.
Complete minicircle genome ofLeptomonas pyrrhocorisreveals sources of its non-canonical mitochondrial RNA editing events
Authors: Gerasimov Evgeny S , Gasparyan Anna A , Afonin Dmitry A , Zimmer Sara L , Kraeva Natalya , Lukeš Julius , Yurchenko Vyacheslav , Kolesnikov Alexander
Published: February 2021

Uridine insertion/deletion (U-indel) editing of mitochondrial mRNA, unique to the protistan class Kinetoplastea, generates canonical as well as potentially non-productive editing events. While the molecular machinery and the role of the guide (g) RNAs that provide required information for U-indel editing are well understood, little is known about the forces underlying its apparently error-prone nature. Analysis of a gRNA:mRNA pair allows the dissection of editing events in a given position of a given mitochondrial transcript. A complete gRNA dataset, paired with a fully characterized mRNA population that includes non-canonically edited transcripts, would allow such an analysis to be performed globally across the mitochondrial transcriptome. To achieve this, we have assembled 67 minicircles of the insect parasite Leptomonas pyrrhocoris, with each minicircle typically encoding one gRNA located in one of two similar-sized units of different origin. From this relatively narrow set of annotated gRNAs, we have dissected all identified mitochondrial editing events in L. pyrrhocoris, the strains of which dramatically differ in the abundance of individual minicircle classes. Our results support a model in which a multitude of editing events are driven by a limited set of gRNAs, with individual gRNAs possessing an inherent ability to guide canonical and non-canonical editing.
Reactive astrocytes in ALS display diminished intron retention
Authors: Ziff Oliver J , Taha Doaa M , Crerar Hamish , Clarke Benjamin E , Chakrabarti Anob M , Kelly Gavin , Neeves Jacob , Tyzack Giulia E , Luscombe Nicholas M , Patani Rickie
Published: February 2021

Reactive astrocytes are implicated in amyotrophic lateral sclerosis (ALS), although the mechanisms controlling reactive transformation are unknown. We show that decreased intron retention (IR) is common to human-induced pluripotent stem cell (hiPSC)-derived astrocytes carrying ALS-causing mutations in VCP, SOD1 and C9orf72. Notably, transcripts with decreased IR and increased expression are overrepresented in reactivity processes including cell adhesion, stress response and immune activation. This was recapitulated in public-datasets for (i) hiPSC-derived astrocytes stimulated with cytokines to undergo reactive transformation and (ii) in vivo astrocytes following selective deletion of TDP-43. We also re-examined public translatome sequencing (TRAP-seq) of astrocytes from a SOD1 mouse model, which revealed that transcripts upregulated in translation significantly overlap with transcripts exhibiting decreased IR. Using nucleocytoplasmic fractionation of VCP mutant astrocytes coupled with mRNA sequencing and proteomics, we identify that decreased IR in nuclear transcripts is associated with enhanced nonsense mediated decay and increased cytoplasmic expression of transcripts and proteins regulating reactive transformation. These findings are consistent with a molecular model for reactive transformation in astrocytes whereby poised nuclear reactivity-related IR transcripts are spliced, undergo nuclear-to-cytoplasmic translocation and translation. Our study therefore provides new insights into the molecular regulation of reactive transformation in astrocytes.
Increased yields of duplex sequencing data by a series of quality control tools
Authors: Povysil Gundula , Heinzl Monika , Salazar Renato , Stoler Nicholas , Nekrutenko Anton , Tiemann-Boege Irene
Published: February 2021

Duplex sequencing is currently the most reliable method to identify ultra-low frequency DNA variants by grouping sequence reads derived from the same DNA molecule into families with information on the forward and reverse strand. However, only a small proportion of reads are assembled into duplex consensus sequences (DCS), and reads with potentially valuable information are discarded at different steps of the bioinformatics pipeline, especially reads without a family. We developed a bioinformatics toolset that analyses the tag and family composition with the purpose to understand data loss and implement modifications to maximize the data output for the variant calling. Specifically, our tools show that tags contain polymerase chain reaction and sequencing errors that contribute to data loss and lower DCS yields. Our tools also identified chimeras, which likely reflect barcode collisions. Finally, we also developed a tool that re-examines variant calls from raw reads and provides different summary data that categorizes the confidence level of a variant call by a tier-based system. With this tool, we can include reads without a family and check the reliability of the call, that increases substantially the sequencing depth for variant calling, a particular important advantage for low-input samples or low-coverage regions.
A prospective phase II randomized trial of proton radiotherapy vs intensity-modulated radiotherapy for patients with newly diagnosed glioblastoma
Authors: Brown Paul D , Chung Caroline , Liu Diane D , McAvoy Sarah , Grosshans David , Al Feghali Karine , Mahajan Anita , Li Jing , McGovern Susan L , McAleer Mary-Fran , Ghia Amol J , Sulman Erik P , Penas-Prado Marta , de Groot John F , Heimberger Amy B , Wang Jihong , Armstrong Terri S , Gilbert Mark R , Guha-Thakurta Nandita , Wefel Jeffrey S
Published: February 2021

Background

To determine if proton radiotherapy (PT), compared to intensity-modulated radiotherapy (IMRT), delayed time to cognitive failure in patients with newly diagnosed glioblastoma (GBM).

Methods

Eligible patients were randomized unblinded to PT vs IMRT. The primary endpoint was time to cognitive failure. Secondary endpoints included overall survival (OS), intracranial progression-free survival (PFS), toxicity, and patient-reported outcomes (PROs).

Results

A total of 90 patients were enrolled and 67 were evaluable with median follow-up of 48.7 months (range 7.1-66.7). There was no significant difference in time to cognitive failure between treatment arms (HR, 0.88; 95% CI, 0.45-1.75; P = .74). PT was associated with a lower rate of fatigue (24% vs 58%, P = .05), but otherwise, there were no significant differences in PROs at 6 months. There was no difference in PFS (HR, 0.74; 95% CI, 0.44-1.23; P = .24) or OS (HR, 0.86; 95% CI, 0.49-1.50; P = .60). However, PT significantly reduced the radiation dose for nearly all structures analyzed. The average number of grade 2 or higher toxicities was significantly higher in patients who received IMRT (mean 1.15, range 0-6) compared to PT (mean 0.35, range 0-3; P = .02).

Conclusions

In this signal-seeking phase II trial, PT was not associated with a delay in time to cognitive failure but did reduce toxicity and patient-reported fatigue. Larger randomized trials are needed to determine the potential of PT such as dose escalation for GBM and cognitive preservation in patients with lower-grade gliomas with a longer survival time.
Parental Anti-Smoking Encouragement as a Longitudinal Predictor of Young Adult Cigarette and E-cigarette Use in a US National Study
Authors: Broun Aaron , Haynie Denise , Choi Kelvin
Published: February 2021

Introduction

Young adulthood is a critical period for the adoption of risk behaviors like tobacco use. Protective factors in adolescence may promote a tobacco-free transition to young adulthood. We examine associations between the frequency of parental anti-smoking encouragement in adolescence and cigarette and e-cigarette use in young adulthood.

Aims and Methods

We analyzed data from Waves 1 (2009–2010, 10th grade, mean age = 16.2 years) and 5 (2013–2014 mean age = 20.3 years) of the US nationally representative NEXT Generation Health Study (n = 1718). At Wave 1, participants reported how often their parents or guardians encourage them to not smoke cigarettes (1 = Rarely or Never, 7 = Frequently). We used separate weighted multiple logistic regression models to model Wave 5 past 30-day cigarette and e-cigarette use as functions of the frequency of parental anti-smoking encouragement at Wave 1, adjusting for sociodemographic and parenting factors, initial substance use, and peer tobacco use.

Results

The average frequency of parental encouragement to not smoke cigarettes was fairly high (mean = 5.35). At Wave 5, 24.7% and 14.2% of respondents reported cigarette and e-cigarette use in the past 30 days, respectively. Greater frequency of parental anti-smoking encouragement was associated with lower odds of subsequent cigarette smoking (adjusted odds ratio 0.91, 95% confidence interval 0.83, 0.99) but its association with e-cigarette use was not significant (adjusted odds ratio 0.93, 95% confidence interval 0.84, 1.04).

Conclusions

The longitudinal negative association between anti-smoking encouragement and cigarette use suggests that parental anti-tobacco communication could be a long-term protective factor against young adult tobacco use. Our findings may also suggest the importance of product-specific messages in the evolving tobacco use landscape.

Implications

This study builds upon prior investigations of parenting in adolescence as a protective factor against young adult risk behavior. We isolate the frequency of anti-smoking encouragement during adolescence as an actionable factor distinct from other parenting variables. Our findings also suggest that message specificity may be an important factor in parental anti-tobacco communication as youth and young adult tobacco use becomes increasingly dominated by e-cigarettes.
Association Between the Mode of Delivery and Infant Gut Microbiota Composition Up to 6 Months of Age: A Systematic Literature Review Considering the Role of Breastfeeding
Authors: Princisval Luciana , Rebelo Fernanda , Williams Brent L , Coimbra Anna Carolina , Crovesy Louise , Ferreira Ana Lorena , Kac Gilberto
Published: February 2021

Context

Cesarean section (CS), breastfeeding, and geographic location can influence the infant microbiota.

Objective

In this systematic review, evidence of the association between mode of delivery and infant gut microbiota up to 6 months of age was evaluated, as was the role of breastfeeding in this association, according to PRISMA guidelines.

Data Source

The Pubmed, Web of Science, Scopus, Embase, Medical Database, and Open Grey databases were searched.

Data Extraction

A total of 31 observational studies with ≥2 infant stool collections up to the sixth month of age and a comparison of gut microbiota between CS and vaginal delivery (VD) were included.

Data Analysis

Infants born by CS had a lower abundance of Bifidobacterium and Bacteroides spp. at almost all points up to age 6 months. Populations of Lactobacillus, Bifidobacterium longum, Bifidobacterium catenulatum, and Escherichia coli were reduced in infants delivered by CS. Infants born by CS and exclusively breastfed had greater similarity with the microbiota of infants born by VD.

Conclusions

Species of Bifidobacterium and Bacteroides are potentially reduced in infants born by CS. Geographic location influenced bacterial colonization.

Systematic Review Registration

PROSPERO registration no. 42017071285
The Farm Lobby and Agricultural Policy in Japan
Authors: Maclachlan Patricia L. , Shimizu Kay
Published: February 2021

Japanese agricultural policymaking has been changing over the past generation. For much of the postwar era, policy formulation fell under the purview of the powerful “farm lobby”—an iron triangle consisting of farm bureaucrats, conservative politicians, and Japan Agricultural Cooperatives (JA), a nationwide network of agricultural cooperative organizations. For the most part, the lobby prioritized the heavy subsidization of farm household incomes and other market-distorting redistributive measures. But by the end of the twentieth century, severe demographic and economic challenges in the countryside combined with new electoral rules to weaken the relative powers of the farm lobby and generate a gradual shift toward structural reform in the farm sector. In tracing these policy-related developments, which reached new heights during the second administration of Shinzō Abe, this chapter illuminates the significance of the organized farm vote, the empowerment of prime ministerial leadership, and the changing fortunes of agriculture in Japan.
The Bank of Japan
Authors: Park Gene
Published: February 2021

In April 1998, the Bank of Japan (BOJ) gained legal independence. While the primary theoretical justification was to enhance the central bank’s inflation-fighting credibility, the newly independent BOJ immediately confronted a different and unexpected problem: a long and persistent deflation. As the government battled economic stagnation, debates over the extent to which the BOJ should prioritize overcoming deflation and the policies that should be employed to this end led to a profound politicization of monetary policy. This culminated in the Prime Minister Abe’s landslide electoral victory at the end of 2012 in which he campaigned on overcoming deflation, and then, once in power, effectively took over control of a previously intransigent BOJ Policy Board to reflate the economy. The democratic electoral process paved the way for a reassertion of control over the still legally independent central bank. From a wider perspective, these developments reflect broader changes in Japanese democracy: the greater influence of electoral incentives on policy and the centralization of executive power.