bluuebunny/crossref_metadata_2025 huggingface dataset:
Linking Error Estimation in Stocking–Lord Linking
Authors: Robitzsch Alexander
Published: December 2024

Stocking–Lord (SL) linking is a widely used linking method based on item response theory (IRT). This article examines the variability in SL linking parameter estimates within the two-parameter logistic (2PL) model. The uncertainty in SL linking arises from the sampling variability (standard error) and item selection (linking error), which can induce variability due to random differential item functioning (DIF). Three linking error estimation approaches are compared in this paper: the conventional jackknife linking error method, a newly developed approximate jackknife linking error method, and a Taylor approximation-based estimate. Simulation studies showed that the approximate jackknife method closely aligns with the traditional jackknife linking error method and outperforms the linking error estimation approach based on Taylor approximation. The adequacy of coverage rates for SL linking parameter estimates was also assessed using estimates of the total error. Results from a simulation study demonstrate that the bias-corrected total error provides superior coverage rates compared to both the conventional total error and the standard error, which does not account for item-related uncertainty due to random DIF.
Fractal-Based Robotic Trading Strategies Using Detrended Fluctuation Analysis and Fractional Derivatives: A Case Study in the Energy Market
Authors: Popovska Ekaterina , Georgieva-Tsaneva Galya
Published: December 2024

This paper presents an integrated robotic trading strategy developed for the day-ahead energy market that includes different methods for time series analysis and forecasting, such as Detrended Fluctuation Analysis (DFA), Rescaled Range Analysis (R/S analysis), fractional derivatives, Long Short-Term Memory (LSTM) Networks, and Seasonal Autoregressive Integrated Moving Average (SARIMA) models. DFA and R/S analysis may capture the long-range dependencies and fractal features inherited by the nature of the electricity price time series and give information about persistence and variability in their behavior. Given this, fractional derivatives can be used to analyze price movements concerning the minor changes in price and time acceleration for that change, which makes the proposed framework more flexible for quickly changing market conditions. LSTM, from their perspective, may capture complex and non-linear dependencies, while SARIMA models may help handle seasonal trends. This integrated approach improves market signal interpretation and optimizes the market risk through adjustable stop-loss and take-profit levels which could lead to better portfolio performance. The proposed integrated strategy is based on actual data from the Bulgarian electricity market for the years 2017–2024. Findings from this research show how the combination of fractals with statistical and machine learning models can improve complex trading strategies implementation for the energy markets.
Sodium Nitroprusside: The Forgotten Vasodilator? A Brief Guide for Informed and Safe Use from Heart Failure to Hypertensive Crisis and Aortic Dissection
Authors: D’Elia Saverio , Franzese Rosa , Gentile Carmine , Solimene Achille , Luisi Ettore , Caiazzo Antonio , Natale Francesco , Loffredo Francesco S. , Golino Paolo , Cimmino Giovanni
Published: December 2024

Sodium nitroprusside (SNP) is a powerful vasodilator approved for treating acute hypertensive crises, acute heart failure, aortic dissection, and both controlled perioperative hypotension and perioperative hypertension. Its unique ability to cause both venous and arterial dilation makes it an invaluable option in critical care settings. Despite concerns regarding its manageability due to potential toxicity, it is a safe choice if properly used, as highlighted by its short half-life and minimal side effects. This review aims to summarize its pharmacological properties, toxicology, and various clinical applications, particularly focusing on acute heart failure and hypertensive emergencies.
Formulation of Hydrogel Beads to Improve the Bioaccessibility of Bioactive Compounds from Goldenberry and Purple Passion Fruit and Evaluation of Their Antiproliferative Effects on Human Colorectal Carcinoma Cells
Authors: Naranjo-Durán Ana María , Miedes Diego , Patiño-Osorio Juan Manuel , Cilla Antonio , Alegría Amparo , Marín-Echeverri Catalina , Quintero-Quiroz Julián , Ciro-Gómez Gelmy Luz
Published: December 2024

Goldenberry and purple passion fruit contain bioactive compounds (BCs) that can prevent gastrointestinal cancers; hydrogel beads can protect and control their release in the gastrointestinal tract. This study aimed to develop an encapsulating material for fruit hydrogel beads (FHBs) to increase their bioaccessibility and to assess antiproliferative effects. A blend of goldenberry–purple passion fruit was encapsulated using ionic gelation and electrospraying. Through a mixture experimental design with sodium alginate (SA), hydroxypropylmethylcellulose (HPMC) and arabic gum (AG) as components, the following response variables were optimized: polyphenol bioaccessibility and encapsulation efficiency. Polyphenols and antioxidant activity were quantified before and after digestion. Antiproliferative effect was evaluated on Caco-2 colon cancer cells. Variations in formulation proportions had a significant effect (p < 0.05) on most responses. An SA-AG mixture in a 0.75:0.25 ratio maximized polyphenol bioaccessibility to 213.17 ± 19.57% and encapsulation efficiency to 89.46 ± 6.64%. Polyphenols and antioxidant activity were lower in FHBs than in the fruit blend (F). Both F and FHBs inhibited tumor cell proliferation by 17% and 25%, respectively. In conclusion, encapsulating BCs in hydrogel beads with SA-AG can enhance the effectiveness of polyphenols in food applications by improving their bioaccessibility and showing a more pronounced effect in inhibiting tumor cell proliferation.
In Vitro Investigation of Novel Peptide Hydrogels for Enamel Remineralization
Authors: Sarosi Codruta , Muntean Alexandrina , Cuc Stanca , Petean Ioan , Balint Sonia , Moldovan Marioara , Mohan Aurel George
Published: December 2024

This study investigates the microstructure of dental enamel following demineralization and re-mineralization processes, using DIAGNOdent scores and images obtained via scanning electron microscopy (SEM), atomic force microscopy (AFM), and microhardness (Vickers). The research evaluates the effects of two experimental hydrogels, Anti-Amelogenin isoform X (ABT260, S1) and Anti-Kallikrein L1 (K3014, S2), applied to demineralized enamel surfaces over periods of 14 and 21 days. The study involved 60 extracted teeth, free from cavities or other lesions, divided into four groups: a positive group (+), a negative group (−) and groups S1 and S2. The last three groups underwent demineralization with 37% phosphoric acid for 20 min. The negative group (−) was without remineralization treatment. The DIAGNOdent scores indicate that the S1 group treated with Anti-Amelogenin is more effective in remineralizing the enamel surface compared to the S2 group treated with Anti-Kallikrein. These findings were corroborated by SEM and AFM images, which revealed elongated hydroxyapatite (HAP) nanoparticles integrated into the demineralized structures. Demineralization reduced enamel microhardness to about 1/3 of a healthy one. Both tested hydrogels restored enamel hardness, with S1 being more effective than S2. Both peptides facilitated the interaction between the newly added minerals and residual protein binders on the enamel surface, thereby contributing to effective enamel restoration.
Publisher’s Note: About Issue 2, Volume 4, 2023
Authors: Lin Shu-Kun
Published: December 2024

According to our publication standards, each journal issue should contain at least one paper [...]
A Path Analysis Study on the Influence of Social Norms on Substance Use Severity: Focusing on People Who Use Cannabis, Narcotics, and Psychotropic Substances in South Korea
Authors: Lee Songhee , Baik Hyung-Ui , Lee Juyong , Shin Yunjae
Published: December 2024

This study investigates the effects of social norms on substance use severity mediated by health beliefs among people who use cannabis, narcotics, and psychotropic substances in Republic of Korea. A survey was administered to 109 people who use cannabis and narcotics and 191 people who use psychotropic substances between May and July 2024. Path analysis was conducted. The findings indicated that the effects of social norms on health beliefs and the impact of health beliefs on substance use severity were statistically significant among people who use psychotropic substances, whereas no such significance was observed among people who use cannabis and narcotics. We recommend implementing early intervention treatment and social rehabilitation programs tailored to the specific needs and severity of substance use in people who use psychotropic substances, cannabis, and narcotics in Republic of Korea. Additionally, the establishment of online platforms to disseminate information on harm reduction, prevention, treatment, and rehabilitation based on substance type is advocated.
Enhancing the Resilience of Agroecosystems Through Improved Rhizosphere Processes: A Strategic Review
Authors: Asghar Waleed , Craven Kelly D. , Swenson Jacob R. , Kataoka Ryota , Mahmood Ahmad , Farias Júlia Gomes
Published: December 2024

As farming practices evolve and climate conditions shift, achieving sustainable food production for a growing global population requires innovative strategies to optimize environmentally friendly practices and minimize ecological impacts. Agroecosystems, which integrate agricultural practices with the surrounding environment, play a vital role in maintaining ecological balance and ensuring food security. Rhizosphere management has emerged as a pivotal approach to enhancing crop yields, reducing reliance on synthetic fertilizers, and supporting sustainable agriculture. The rhizosphere, a dynamic zone surrounding plant roots, hosts intense microbial activity fueled by root exudates. These exudates, along with practices such as green manure application and intercropping, significantly influence the soil’s microbial community structure. Beneficial plant-associated microbes, including Trichoderma spp., Penicillium spp., Aspergillus spp., and Bacillus spp., play a crucial role in improving nutrient cycling and promoting plant health, yet their interactions within the rhizosphere remain inadequately understood. This review explores how integrating beneficial microbes, green manures, and intercropping enhances rhizosphere processes to rebuild microbial communities, sequester carbon, and reduce greenhouse gas emissions. These practices not only contribute to maintaining soil health but also foster positive plant–microbe–rhizosphere interactions that benefit entire ecosystems. By implementing such strategies alongside sound policy measures, sustainable cropping systems can be developed to address predicted climate challenges. Strengthening agroecosystem resilience through improved rhizosphere processes is essential for ensuring food security and environmental sustainability in the future. In conclusion, using these rhizosphere-driven processes, we could develop more sustainable and resilient agricultural systems that ensure food security and environmental preservation amidst changing climate situations.
In Silico Analysis Revealed Marco (SR-A6) and Abca1/2 as Potential Regulators of Lipid Metabolism in M1 Macrophage Hysteresis
Authors: Zhang Yubo , Yang Wenbo , Kumagai Yutaro , Loza Martin , Yang Yitao , Park Sung-Joon , Nakai Kenta
Published: December 2024

Macrophages undergo polarization, resulting in distinct phenotypes. These transitions, including de-/repolarization, lead to hysteresis, where cells retain genetic and epigenetic signatures of previous states, influencing macrophage function. We previously identified a set of interferon-stimulated genes (ISGs) associated with high lipid levels in macrophages that exhibited hysteresis following M1 polarization, suggesting potential alterations in lipid metabolism. In this study, we applied weighted gene co-expression network analysis (WGCNA) and conducted comparative analyses on 162 RNA-seq samples from de-/repolarized and lipid-loaded macrophages, followed by functional exploration. Our results demonstrate that during M1 hysteresis, the sustained high expression of Marco (SR-A6) enhances lipid uptake, while the suppression of Abca1/2 reduces lipid efflux, collectively leading to elevated intracellular lipid levels. This accumulation may compensate for reduced cholesterol biosynthesis and provide energy for sustained inflammatory responses and interferon signaling. Our findings elucidate the relationship between M1 hysteresis and lipid metabolism, contributing to understanding the underlying mechanisms of macrophage hysteresis.
Optimized Prime Editing of Human Induced Pluripotent Stem Cells to Efficiently Generate Isogenic Models of Mendelian Diseases
Authors: Cerna-Chavez Rodrigo , Ortega-Gasco Alba , Baig Hafiz Muhammad Azhar , Ehrenreich Nathan , Metais Thibaud , Scandura Michael J. , Bujakowska Kinga , Pierce Eric A. , Garita-Hernandez Marcela
Published: December 2024

Prime editing (PE) is a CRISPR-based tool for genome engineering that can be applied to generate human induced pluripotent stem cell (hiPSC)-based disease models. PE technology safely introduces point mutations, small insertions, and deletions (indels) into the genome. It uses a Cas9-nickase (nCas9) fused to a reverse transcriptase (RT) as an editor and a PE guide RNA (pegRNA), which introduces the desired edit with great precision without creating double-strand breaks (DSBs). PE leads to minimal off-targets or indels when introducing single-strand breaks (SSB) in the DNA. Low efficiency can be an obstacle to its use in hiPSCs, especially when the genetic context precludes the screening of multiple pegRNAs, and other strategies must be employed to achieve the desired edit. We developed a PE platform to efficiently generate isogenic models of Mendelian disorders. We introduced the c.25G>A (p.V9M) mutation in the NMNAT1 gene with over 25% efficiency by optimizing the PE workflow. Using our optimized system, we generated other isogenic models of inherited retinal diseases (IRDs), including the c.1481C>T (p.T494M) mutation in PRPF3 and the c.6926A>C (p.H2309P) mutation in PRPF8. We modified several determinants of the hiPSC PE procedure, such as plasmid concentrations, PE component ratios, and delivery method settings, showing that our improved workflow increased the hiPSC editing efficiency.
Key Interleukins in Inflammatory Bowel Disease—A Review of Recent Studies
Authors: Aebisher David , Bartusik-Aebisher Dorota , Przygórzewska Agnieszka , Oleś Piotr , Woźnicki Paweł , Kawczyk-Krupka Aleksandra
Published: December 2024

Inflammatory bowel disease (IBD) is an immune disorder of the gastrointestinal tract with a complex aetiopathogenesis, whose development is influenced by many factors. The prevalence of IBD is increasing worldwide, in both industrialized and developing countries, making IBD a global health problem that seriously affects quality of life. In 2019, there were approximately 4.9 million cases of IBD worldwide. Such a large number of patients entails significant healthcare costs. In the treatment of patients with IBD, the current therapeutic target is mucosal healing, as intestinal inflammation often persists despite resolution of abdominal symptoms. Treatment strategies include amino salicylates, corticosteroids, immunosuppressants, and biologic therapies that focus on reducing intestinal mucosal inflammation, inducing and prolonging disease remission, and treating complications. The American College of Gastroenterology (ACG) guidelines also indicate that nutritional therapies may be considered in addition to other therapies. However, current therapeutic approaches are not fully effective and are associated with various limitations, such as drug resistance, variable efficacy, and side effects. As the chronic inflammation that accompanies IBD is characterized by infiltration of a variety of immune cells and increased expression of a number of pro-inflammatory cytokines, including IL-6, TNF-α, IL-12, IL-23 and IFN-γ, new therapeutic approaches are mainly targeting immune pathways. Interleukins are one of the molecular targets in IBD therapy. Interleukins and related cytokines serve as a means of communication for innate and adaptive immune cells, as well as nonimmune cells and tissues. These cytokines play an important role in the pathogenesis and course of IBD, making them promising targets for current and future therapies. In our work, we review scientific studies published between January 2022 and November 2024 describing the most important interleukins involved in the pathogenesis of IBD. Some of the papers present new data on the precise role that individual interleukins play in IBD. New clinical data have also been provided, particularly on blocking interleukin 23 and interleukin 1beta. In addition, several new approaches to the use of different interleukins in the treatment of IBD have been described in recent years.
Transcriptional Changes Associated with Amyoplasia
Authors: Komissarov Artem E. , Agranovich Olga E. , Kuchinskaia Ianina A. , Tkacheva Irina V. , Bolshakova Olga I. , Latypova Evgenia M. , Batkin Sergey F. , Sarantseva Svetlana V.
Published: December 2024

Arthrogryposis, which represents a group of congenital disorders, includes various forms. One such form is amyoplasia, which most commonly presents in a sporadic form in addition to distal forms, among which hereditary cases may occur. This condition is characterized by limited joint mobility and muscle weakness, leading to limb deformities and various clinical manifestations. At present, the pathogenesis of this disease is not clearly understood, and its diagnosis is often complicated due to significant phenotypic diversity, which can result in delayed detection and, consequently, limited options for symptomatic treatment. In this study, a transcriptomic analysis of the affected muscles from patients diagnosed with amyoplasia was performed, and more than 2000 differentially expressed genes (DEGs) were identified. A functional analysis revealed disrupted biological processes, such as vacuole organization, cellular and aerobic respiration, regulation of mitochondrion organization, cellular adhesion, ATP synthesis, and others. The search for key nodes (hubs) in protein–protein interaction networks allowed for the identification of genes involved in mitochondrial processes.
γ-Aminobutyric Acid Alleviates Programmed Cell Death in Two Brassica Species Under Cadmium Stress
Authors: Zhang Zhong-Wei , Dang Tao-Tao , Yang Xin-Yue , Xie Lin-Bei , Chen Yang-Er , Yuan Ming , Chen Guang-Deng , Zeng Jian , Yuan Shu
Published: December 2024

Previous studies have demonstrated that γ-Aminobutyric acid (GABA) effectively alleviates heavy metal stresses by maintaining the redox balance and reducing the accumulation of reactive oxygen species (ROS). However, little is known about the role of GABA on programmed cell death (PCD) under Cd treatments in plants. The present study investigated the effects of GABA on Cd-induced PCD in two Brassica species, oilseed rape (Brassica napus, Bn), and black mustard (Brassica juncea, Bj). We observed that GABA significantly alleviated Cd-induced PCD by enhancing antioxidant systems, inhibiting chromatin condensation in the nucleus, and reducing DNA fragmentation under Cd stress. Moreover, GABA may not only reduce caspase-3-like activity by repressing gene expression, but also regulate transcription of PCD-related genes. Bn showed lower Cd accumulation and lower tolerance, with more pronounced PCD, compared with Bj. Our results provide new insights into the mechanism that GABA enhances Cd tolerance in plants.
Genome-Wide Identification and Expression Analysis of NF-YA Gene Family in the Filling Stage of Wheat (Triticum aestivum L.)
Authors: Zhang Yang , Xu Yanmin , Mao Yulu , Tan Xiaodi , Tian Yuan , Ma Xiaofei , Ji Hutai , Zhang Dingyi
Published: December 2024

The NF-YA gene family is a highly conserved transcription factor that plays a crucial role in regulating plant growth, development, and responses to various stresses. Despite extensive studies in multiple plants, there has been a dearth of focused and systematic analysis on NF-YA genes in wheat grains. In this study, we carried out a comprehensive bioinformatics analysis of the NF-YA gene family in wheat, using the latest genomic data from the Chinese Spring. A total of 19 TaNF-YA genes were identified. An analysis of conserved domains, phylogenetic relationships, and gene structure indicated a significant degree of conservation among TaNF-YAs. A gene collinearity analysis demonstrated that fragment duplication was the predominant mechanism driving the amplification of TaNF-YAs. Furthermore, cis-acting elements within the promoters of TaNF-YAs were found to be implicated in grain development. Subsequently, SNP analysis revealed the genetic variation in the NF-YA gene family in different wheat. Moreover, published RNA-seq data were used and RNA-seqs of Pinyu8155, Yaomai30, Yaomai36, and Pinyu8175 were performed to identify TaNF-YAs influencing grain development. Finally, it was found that NF-YAs had no self-activating activity in wheat. This study provides key candidate genes for the exploration of grain development in the wheat filling stage and also lays a foundation for further research on the regulation of starch and protein synthesis and accumulation.
Expanding Upon Genomics in Rare Diseases: Epigenomic Insights
Authors: Tan Jia W. , Blake Emily J. , Farris Joseph D. , Klee Eric W.
Published: December 2024

DNA methylation is an essential epigenetic modification that plays a crucial role in regulating gene expression and maintaining genomic stability. With the advancement in sequencing technology, methylation studies have provided valuable insights into the diagnosis of rare diseases through the various identification of episignatures, epivariation, epioutliers, and allele-specific methylation. However, current methylation studies are not without limitations. This mini-review explores the current understanding of DNA methylation in rare diseases, highlighting the key mechanisms and diagnostic potential, and emphasizing the need for advanced methodologies and integrative approaches to enhance the understanding of disease progression and design more personable treatment for patients, given the nature of rare diseases.
Beyond Cations: Expanding the Horizons of Ferrocene-Based Electrochemical Sensors for Neutral and Anionic Molecules
Authors: Torriero Angel A. J. , Torriero Alma M. , Miller Kiara T. , Mruthunjaya Ashwin K. V.
Published: December 2024

Ferrocene (Fc) has long been celebrated for its remarkable redox properties and structural versatility, making it a cornerstone of electrochemical sensor development. While extensive research has focused on cation detection using Fc-based systems, the equally critical recognition of neutral and anionic molecules remains underexplored despite their significance in biological, environmental, and industrial contexts. This review addresses this gap by exploring the latest advancements in Fc-based electrochemical sensors designed to overcome the unique challenges posed by these species—including diverse geometries, high hydration enthalpies, and the absence of formal charge. Molecular architectures such as amide-functionalised receptors, urea derivatives, Lewis acid-containing receptors, triazolium, and carboxylic acid-containing systems are examined, highlighting how these sensors achieve high selectivity and sensitivity. Furthermore, the influence of solvent environments on sensor performance is discussed, providing a critical analysis of how different receptor functionalities and solvents affect sensor behaviour. Emphasising the advantages of redox-based detection, this review aims to inspire further innovation in developing Fc-based technologies for detecting neutral and anionic species.
Optimizing Performance in Badminton Tournaments: The Relationship Between Timing, Quantity, and Quality Among Professional Players
Authors: Hsu Jen-Hao , Lin Hsin-Lun , Fan Chiang Hung-Chieh , Lee Duan-Shin , Lee Yang , Huang Cheng-Wei , Yao Zai-Fu
Published: December 2024

Background/Objectives: Top badminton players must carefully schedule tournaments to perform well and improve their rankings. This study examines how players plan their tournament participation and whether their scheduling strategies affect their rankings and performance, especially during Olympic and non-Olympic years. Methods: Data were extracted from the Badminton World Federation (BWF) ranking system for the top 50 men’s and women’s singles players from May 2014 to May 2019. A computer-based simulation model and cluster analysis were applied to analyze tournament participation patterns, recovery intervals, and performance trends. Statistical analyses were conducted to identify correlations between these variables and ranking outcomes. Results: Top-ranked players participated in more tournaments during Olympic years, with those competing in more than nine tournaments achieving better results. Players performing well in higher-graded tournaments tended to take longer recovery breaks, whereas lower-performing players entered more tournaments. Cluster analysis revealed two distinct performance groups, with the top 20 men’s and top 12 women’s singles players adopting different strategies compared to lower-ranked counterparts. Conclusions: Strategic scheduling of tournaments, including balancing participation frequency and recovery intervals, is vital for optimizing performance and achieving sustained ranking success. These findings emphasize the importance of high-graded tournament selection and tailored schedules for elite players.
Special Issue “Exercise and Neurodegenerative Disease 2.0”
Authors: Maugeri Grazia , D’Agata Velia
Published: December 2024

It is well known that sedentary life is detrimental for human health; on the contrary, an active lifestyle represents an efficient instrument to guarantee and promote physical and psychological health [...]
Therapeutic Physical Exercise for Dysmenorrhea: A Scoping Review
Authors: Rigal Philippine , Bonnet Salomé , Vieira Ágata , Carvalhais Alice , Lopes Sofia
Published: December 2024

Background: Dysmenorrhea affects many women of reproductive age. Physical exercise has been used as an effective intervention for pain reduction and to improve well-being. Physiotherapy, involving movement and exercise, can be effective in relieving menstrual pain and provide additional benefits. The aim is to identify therapeutic physical exercise program and exercise protocols used to reduce pain among these women. Methods: A scoping review was conducted in accordance with Joanna Briggs Institute’s methodology, using the PCC acronym. Articles were sourced from: PubMed, Cochrane Library, PEDro and ScienceDirect, covering studies published between 1 January 2013 and 30 April 2023, representing the period we considered most appropriate at the time the study was initiated. Qualitative studies, books, book chapters, systematic reviews, meta-analyses and review articles were excluded. Studies were analyzed according to the PRISMA-ScR framework. Results: 3325 studies were identified, but only 9 were included. Considerable variation was observed in the types and parameters of the exercise program across studies, including differences in duration, intensity, number of repetitions and series. Conclusions: The findings of this study highlight that aerobic training, particularly among women in their 20s, emerged as the most frequently utilized form of therapeutic physical exercise for alleviating menstrual pain in the studies reviewed. This suggests that aerobic exercise may hold significant promise as a non-pharmacological intervention for managing dysmenorrhea.
Effect of Post-Processing on the Microstructure of WE43 Magnesium Alloy Fabricated by Laser Powder Directed Energy Deposition
Authors: Sorkhi Leila , Madden Nathan J. , Crawford Grant A.
Published: December 2024

Additive manufacturing of magnesium (Mg) alloys is of interest for the fabrication of complex-shaped lightweight materials. This study evaluates the microstructure of WE43 Mg alloy deposited using laser powder directed energy deposition (LPDED) additive manufacturing technique in as-deposited and post-processed conditions. As-deposited samples exhibited roughly 2% porosity, which was reduced to below 0.1% after hot isostatic pressing. Despite limited grain growth after heat treatment, some grains experienced abnormal grain growth, likely due to Zener pinning and non-uniform dissolution of grain boundary precipitates. Moreover, as-deposited specimens contained Nd-rich grain boundary precipitates which dissolved during post-processing. Additionally, during heat treatment. a fine distribution of needle-like β1 or β precipitates formed. Overall, the precipitate size and distribution following heat treatment was non-uniform, likely because of the non-uniform response of the LPDED material to heat treatment, owing to the variation in local- and global-temperature profiles during deposition. Furthermore, arc-shaped phases with a high concentration of Y, O, and Zr were present for all processing conditions and are associated with the passivation of the feedstock powder prior to deposition. Moreover, an equiaxed-grain structure with a random orientation and a finer grain size in the regions adjacent to the arc-shaped phases was observed in all processing conditions.
Pressure-Less Liquid-Phase Sintering of Aluminum-Based Materials
Authors: Sucgang Ana Teresa , Cuzacq Laurent , Bobet Jean-Louis , Lu Yongfeng , Silvain Jean-François
Published: December 2024

Rapid technological advancements and the growing focus on sustainable practices have significantly expanded the potential applications of aluminum (Al) and its alloys, leading to a steady increase in demand over the years. This study investigated the densification of Al and Al-based materials using pressure-less liquid-phase sintering. Samples with 4–20 vol.% AlSi12 sintered at 640 °C for 1 h achieved the highest relative density (RD) and the lowest global porosity (GP) without exhibiting any shape deformation. In general, increasing the amount of sintering aid improves the density of the samples. This was confirmed by microstructural analysis using SEM, which revealed the progression of density—from initial particle coalescence at 4 vol.% AlSi12 to the development of microstructures with filled pores and well-defined grain boundaries at 20 vol.% AlSi12. X-ray diffraction (XRD) analysis also revealed an expanded lattice parameter, with minimal microstrain and a crystallite size closely resembling those of the initial Al powder. Samples with a relative density greater than 90% demonstrated thermal conductivities ranging from 170 to 200 W/mK and an average hardness of 29 HV5. Densification was further enhanced by increasing the compaction pressure from 50 MPa to 100–200 MPa for samples containing 12–20 vol.% AlSi12. The Al-based material compacted at 200 MPa and with 15 vol.% AlSi12 achieved the highest RD of approximately 99%. It exhibited a thermal conductivity of 195 W/mK at 30 °C and 190 W/mK at 70 °C, along with a hardness of 30 HV5.
Dimensional Analysis and Validity of Uniaxial Residual Stress Distribution for Welded Box Sections
Authors: Horváth András , Kollár Dénes
Published: December 2024

This paper investigates the residual stresses induced by metal inert/active gas (MIG/MAG) welding in normal strength steel box sections, focusing on the validity of uniaxial residual stress assumption. Advanced manufacturing simulations are conducted using deterministic uncoupled transient thermomechanical analysis with a double-ellipsoidal heat source model, employing 8-node solid elements and material models calibrated for extreme temperatures per EN 1993-1-2. A comprehensive parametric analysis investigates the effects of primary welding variables, such as heat source power and welding speed, alongside geometric parameters of the heat source model using random Latin hypercube sampling technique in the analyzed parameter set. The relationship between the size and shape of the characteristic isotherms, i.e., the aspect ratio and the Rosenthal number, underscores that the analyzed welding heat sources are in the fast regime with the validity of uniaxial residual stresses based on the analytical assumption (minimal values are AR = 9.94 and Ro = 30.47). The validity and limitations of uniaxial residual stress assumptions for 59 welded and 51 heated box sections are critically evaluated by using the finite element model-based stress triaxiality parameter. Results confirm that longitudinal residual stresses dominate typical MIG/MAG welding applications, supporting the application of uniaxial residual stress models in advanced structural design by neglecting in-plane and through-thickness residual stresses. Conversely, three-dimensional residual stress state dominates under conditions such as preheating or thermal straightening.
Correction: Cai et al. Roles of Three FgPel Genes in the Development and Pathogenicity Regulation of Fusarium graminearum. J. Fungi 2024, 10, 666
Authors: Cai Lu , Xu Xiao , Dong Ye , Jin Yingying , Rashad Younes M. , Ma Dongfang , Gu Aiguo
Published: December 2024

In the original article [...]
A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of Phytophthora sojae
Authors: Du Xiaoran , Zeng Yan , Li Yiying , Peng Qin , Miao Jianqiang , Liu Xili
Published: December 2024

While the rapid rise in bioinformatics has facilitated the identification of the domains and functions of many proteins, some still have no domain annotation or largely uncharacterized functions. However, the biological roles of unknown proteins were not clear in oomycetes. An analysis of the Phytophthora sojae genome database identified the protein Ps495620, which has no domain annotations and functional predictions in Phytophthora. This study used a CRISPR/Cas9-mediated gene replacement system to knock out Ps495620 to elucidate its function. The Ps495620-knockout mutants exhibited significantly increased oospore production and decreased sporangium formation compared to the wild-type strain P6497. Transcriptomics showed that it is a key regulator of nitrogen, pyruvate, ascorbate, and adorate metabolism in P. sojae. Our findings indicate that Ps495620 is critical in regulating sporangium formation and oospore production in P. sojae.
Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea
Authors: Zhang Lu , Bai Jun , Guo Qian , Li Long , Jia Yanqing , Qiu Xinxin , Zhou Dong , Zhang Zhencang , Niu Huafeng
Published: December 2024

Calf diarrhea is a prevalent and significant health issue in dairy farming, severely impacting feed intake, weight gain, and survival rates in young calves. This study aimed to investigate the microbial composition and antibiotic resistance profiles of diarrheic calves to provide insights into the epidemiology and management of the condition. The prevalence of diarrhea in 1685 calves was analyzed. Rectal fecal samples were collected from five healthy and five diarrheic Holstein calves on a large dairy farm in Shaanxi Province, China. High-throughput 16S-rRNA sequencing and PCR were utilized for microbial and resistance gene analysis. In 2023, the overall diarrhea rate among 1685 calves was 9.08%, with a significantly higher diarrhea rate during the suckling period (8.13%) compared to the post-weaning period (0.95%) (p < 0.001). No differences in species diversity and richness were detected among the different groups. However, LEfSe analysis identified six genera (Eubacterium, Eubacteriaceae, Prevotella, Comamonadaceae, Comamonas, and Firmicutes) significantly enriched in diarrheic calves compared to healthy ones (LDA scores > 2, p < 0.05). Additionally, antibiotic resistance genes for quinolones, β-lactams, chloramphenicol, tetracyclines, and aminoglycosides were detected, with significantly higher prevalence in diarrheic calves. These findings demonstrate distinct microbial and antibiotic resistance profiles between healthy and diarrheic calves, emphasizing the importance of microbial management in controlling calf diarrhea.
Assessment of Radiological Safety of Ceramic Tiles Commonly Used in Polish Buildings
Authors: Łukaszek-Chmielewska Aneta , Rachwał Marzena , Rakowska Joanna , Ośko Jakub , Konop Marta , Kosturkiewicz Bogdan , Kosturkiewicz Mateusz , Łapicz Marcin
Published: December 2024

The concentration of natural radionuclides 226Ra, 232Th and 40K in ceramic tiles manufactured in Poland is presented in this paper. The concentration of natural radioactive isotopes in the tested samples was determined using a low-level digital gamma ray spectrometer equipped with an HPGe semiconductor detector. The mean concentrations of 226Ra, 232Th and 40K in the analyzed samples were found to be 48 ± 3 Bq∙kg−1, 49 ± 3 Bq∙kg−1 and 476 ± 23 Bq∙kg−1, respectively. The world mean concentrations of these radionuclides (50 Bq·kg−1, 50 Bq·kg−1 and 500 Bq·kg−1, respectively) were not exceeded. Furthermore, in order to ascertain the level of gamma radiation exposure, fundamental radiation protection parameters were established: radioactivity concentration indicator/gamma ray indicator (Iγ), indoor dose rate (Din) and annual indoor effective dose (Ein). In the case of the investigated ceramic tiles, it was established that the parameters were not higher than the limit values, except the indoor gamma radiation dose rate which was found to be 1.5 times higher than the world average. Therefore, the findings of this study indicate that the utilization of the examined ceramic tiles in constructions should be approached with a degree of caution.
Mix Design and Performance Study of High-Strength Self-Compacting Concrete with Manufactured Sand
Authors: Liu Xuan , Wang Xuhao , Wang Yuan , Liu Qianqian , Tian Yuan , Zhou Jie , Meng Yahong
Published: December 2024

In recent years, research on self-compacting concrete (SCC) has gradually shifted towards high-strength development, while high-strength self-compacting concrete has been widely used in applications such as precast bridge components and high-rise building projects. Using manufactured sand as an aggregate can effectively address the challenges posed by the depletion of natural sand resources. This study optimized the mix design for high-strength self-compacting concrete with manufactured sand (MSH-SCC) and explored the effects of the fine aggregate replacement rate, sand ratio, and maximum particle size of coarse aggregate on the performance of MSH-SCC. The results indicated that the optimized mix designs for various strength levels met the performance requirements. The fine aggregate replacement rate and the maximum nominal aggregate size significantly affected the workability of the concrete, while variations in the sand ratio had a smaller impact. The yield stress of the MSH-SCC showed a positive correlation with the fine aggregate replacement rate and the maximum nominal aggregate size, whereas the plastic viscosity reached its maximum value under specific conditions. Additionally, the mix design parameters had a limited effect on the mechanical strength of the MSH-SCC. This study provides a scientific basis for the design of high-strength self-compacting concrete with manufactured sand, contributing to the promotion of manufactured sand use and advancing low-carbon development in the construction industry.
Experimental Investigation and Modeling of Surface Roughness in BTA Deep Hole Drilling with Vibration Assisted
Authors: Li Xubo , Zhai Chuanmiao , Wang Canjun , Wu Ruiqin , Zang Cunqiang , Zhang Shihao , Guo Bian , Su Yuewen
Published: December 2024

The surface roughness of hole machining greatly influences the mechanical properties of parts, such as early fatigue failure and corrosion resistance. The boring and trepanning association (BTA) deep hole drilling with axial vibration assistance is a compound machining process of the tool cutting and the guide block extrusion. At the same time, the surface of the hole wall is also ironed by the axial large amplitude and low-frequency vibration of the guide block. The surface-forming mechanism is very complicated, making it difficult to obtain an effective theoretical analytical model of the surface roughness of the hole wall through kinematic analysis. In order to achieve accurate prediction of the surface quality of the hole wall, the chip-breaking mechanism and the hole wall formation mode of BTA deep hole vibration drilling were analyzed. The influence of drilling spindle speed, feed, amplitude, and vibration frequency on the surface roughness of the hole wall during BTA deep hole vibration drilling was illustrated by a single-factor experiment. A four-factor and three-level test scheme was designed by using the Box–Behnken design (BBD) experimental design method. A surface roughness prediction model for hole wall machining was established based on the response surface methodology. The accuracy of the prediction model was analyzed through ANOVA, and the complex correlation coefficient of the model was 0.9948, indicating that the prediction model can better reflect the mapping relationship between vibration drilling parameters and surface roughness. After optimization analysis and experimental verification, the obtained vibration drilling parameters can achieve smaller surface roughness. The error between the predicted value of the model and the experimental measurement value is 8.65%. The established prediction model is reliable and can accurately predict the surface roughness of the hole wall of BTA deep hole axial vibration drilling, providing a theoretical basis for the surface quality control of the machining hole wall. It can be applied to process optimization in practical production.
Development of Application Customization Toolkit (ACT) for 3D Thermal Elastic-Plastic Welding Analysis
Authors: Lee Jaeyong , Park Dong Hee , Park Juhyeon , Kim Do Kyun
Published: December 2024

A 3D thermal elastic-plastic welding analysis ACT (Application Customization Toolkit) was developed in ANSYS, making welding analysis more accessible. The welding analysis was performed using a decoupled method, separated into thermal and structural analyses. To validate the results, comparisons were made with previous studies for two types of welding: T-joint fillet welding and butt welding. Subsequently, the residual stress and deformation obtained from the welding analysis were applied as initial imperfections in a compression analysis to evaluate the ultimate compressive strength with conventional compression analysis. This comparison allowed for a more realistic assessment of the effects of deformation and residual stress distribution on the structural behaviours.
Identifying Crystal Structure of Halides of Strontium and Barium Perovskite Compounds with EXPO2014 Software
Authors: Perez Franco Jorge A. , García Murillo Antonieta , Carrillo Romo Felipe de J. , Romero Ibarra Issis C. , Cervantes Tobón Arturo
Published: December 2024

The synthesis of ethylamine-based perovskites has emerged to attempt to replace the lead in lead-based perovskites for the alkaline earth elements barium and strontium, introducing chloride halide to prepare the perovskites in solar cell technology. X-ray diffraction studies were conducted, and EXPO2014 software was utilized to resolve the structure. Chemical characterization was performed using Fourier transform infrared spectroscopy, photophysical properties were analyzed through ultraviolet–visible spectroscopy, and photoluminescence properties were determined to confirm the perovskite characteristics. The software employed can determine new crystal structures, as follows: orthorhombic for barium perovskite CH3CH2NH3BaCl3 and tetragonal for strontium perovskite CH3CH2NH3SrCl3. The ultraviolet–visible spectroscopy data demonstrated that a temperature increase (90–110 °C) contributed to reducing the band gap from 3.93 eV to 3.67 eV for barium perovskite and from 4.05 eV to 3.84 eV for strontium perovskite. The results exhibited that new materials can be obtained through gentle chemistry and specialized software like EXPO2014, both of which are capable of conducting reciprocal and direct space analyses for identifying crystal structures using powder X-ray diffraction.
Development of a Fire-Retardant and Sound-Insulating Composite Functional Sealant
Authors: Li Shiwen , Wang Mingyu , Tu Jinchun , Wang Bingrong , Wang Xiaohong , Zhang Kexi
Published: December 2024

The use of traditional sealing materials in buildings poses a significant risk of fire and noise pollution. To address these issues, we propose a novel composite functional sealant designed to enhance fire safety and sound insulation. The sealant incorporates a unique four-component filler system consisting of carbon nanotubes (CNTs) decorated with layered double hydroxides (LDHs), ammonium dihydrogen phosphate (ADP), and artificial marble waste powder (AMWP), namely CLAA. The CNTs/LDHs framework provides structural support and enhances thermal stability, while the ADP layer acts as a protective barrier and releases non-combustible gases during combustion. AMWP particles contribute to sound insulation by creating impedance mismatches. The resulting composite functional sealant exhibits improved mechanical properties. In terms of flame retardancy, it boasts the lowest peak heat release rate (PHRR) of 224.83 kW/m2 and total smoke release (TSR) of 981.14 m2/m2, achieving the V-0 classification. Furthermore, its thermal degradation characteristics reveal a notably higher carbon residue rate. Additionally, the sound insulation capability has been significantly enhanced, with an average sound insulation level of 43.48 dB. This study provides a promising solution for enhancing the fire safety and acoustic properties of building sealing materials.
Slow Relaxation of the Magnetisation in a Two-Dimensional Metal–Organic Framework with a Layered Square Lattice
Authors: Benmansour Samia , Cerezo-Navarrete Christian , Gómez-García Carlos J.
Published: December 2024

Herein, we present the synthesis and structural characterisation of two layered MOFs with the asymmetric ligand 3-chloro,6-cyano-2,5-dihydroxy-1,4-benzoquinone dianion (C6O4(CN)Cl2− = chlorocyananilato). These compounds, formulated as (H3O)[Eu(C6O4(CN)Cl)2(H2O)]·34H2O (1) and (H3O)[Dy(C6O4(CN)Cl)2(H2O)]·44H2O (2), are isostructural and show a (4,4)-layered square structure with the crystallisation water molecules located between the layers. The lanthanoid ions are surrounded by four bis-bidentate chlorocyananilato ligands that connect each LnIII centre with other four, giving rise to square cavities formed by LnIII centres in the vertices and chlorocyananilato ligands as the sides. There is an additional coordinated water molecule that occupies the caped position of the capped square antiprismatic coordination geometry around the LnIII centres. The magnetic properties show the presence of a field-induced slow relaxation of the magnetisation in the DyIII derivative at low temperatures that follows Direct and Orbach relaxation mechanisms with an energy barrier of 36(3) K.