bluuebunny/crossref_metadata_2025 huggingface dataset:
Metal–Insulator Transition in Doped Barium Plumbates
Authors: Eufrasio Andreza M. , Pegg Ian , Kafle Amrit , Wong-Ng Winnie , Huang Qingzhen , Dutta Biprodas
Published: September 2021

Solid solutions in the Ba(Pb1−xSrx)O3−z system were prepared by aliovalent substitution of Pb4+ by Sr2+ ions to investigate the effect of cation stoichiometry on thermal and electrical properties as x was varied between 0 and 0.4, in the temperature range 300–523 K. The starting compound, barium plumbate (BaPbO3), has a perovskite structure and is known to exhibit metallic conductivity due to an overlap of the O2p nonbonding and the Pb–O spσ antibonding band, which is partially filled by the available electrons. The large difference in the ionic radii between the Pb4+ and Sr2+ ions introduces significant strain into the (Pb/Sr)O6 octahedra of the perovskite structure. Additionally, charged defects are created on account of the different oxidation states of the Pb4+ and Sr2+ ions. Evidence of a metal to insulator transition (MIT) of the Mott–Hubbard type has been observed at a critical concentration of Sr2+ ions.
Sizing and Management of an Energy System for a Metropolitan Station with Storage and Related District Energy Community
Authors: Simoiu Mircea Stefan , Fagarasan Ioana , Ploix Stephane , Calofir Vasile
Published: September 2021

Future renewable energy communities will reshape the paradigm in which we design and control efficient power systems at the district level. In this manner, the focus will be fundamentally shifted towards sustainable related concepts such as self-consumption, self-sufficiency and net energy exchanged with the grid. In this context, the paper presents a novel approach for optimally designing and controlling the photovoltaic plant and energy storage systems for a metro station in order to increase collective self-consumption and self-sufficiency at the district level. The methodology considers a community of several households connected to a subway station and focuses on the interaction between energy sources and consumers. Furthermore, the optimal solution is determined by using a Mixed Integer Linear Programming Approach, and the impact of different configurations on the overall district benefit is investigated by using several simulation scenarios. The work presents a detailed case study to underline the benefits and flexibility offered by the energy storage system in comparison with a scenario involving only a photovoltaic plant.
Identification of Critical Components in the Complex Technical Infrastructure of the Large Hadron Collider Using Relief Feature Ranking and Support Vector Machines
Authors: Shokry Ahmed , Baraldi Piero , Castellano Andrea , Serio Luigi , Zio Enrico
Published: September 2021

This work proposes a data-driven methodology for identifying critical components in Complex Technical Infrastructures (CTIs), for which the functional logic and/or the system structure functions are not known due the CTI’s complexity and evolving nature. The methodology uses large amounts of CTI monitoring data acquired over long periods of time and under different operating conditions. The critical components are identified as those for which the condition monitoring signals permit the optimal classification of the CTI functioning or failed state. The methodology includes two stages: in the first stage, a feature selection filter method based on the Relief technique is used to rank the monitoring signals according to their importance with respect to the CTI functioning or failed state; the second stage identifies the subset of signals among those highlighted by the Relief technique that are most informative with respect to the CTI state. This identification is performed on the basis of evaluating the performance of a Cost-Sensitive Support Vector Machine (CS-SVM) classifier trained with several subsets of the candidate signals. The capabilities of the methodology proposed are assessed through its application to different benchmarks of highly imbalanced datasets, showing performances that are competitive to those obtained by other methods presented in the literature. The methodology is finally applied to the monitoring signals of the Large Hadron Collider (LHC) of the European Organization for Nuclear Research (CERN), a CTI for experiments of physics; the criticality of the identified components has been confirmed by CERN experts.
Multi-Criteria Optimization of Energy-Efficient Cementitious Sandwich Panels Building Systems Using Genetic Algorithm
Authors: Mirnateghi Ehsan , Mosallam Ayman S.
Published: September 2021

This paper presents results of a study that focuses on developing a genetic algorithm (GA) for multi-criteria optimization of orthotropic, energy-efficient cementitious composite sandwich panels (CSP). The current design concept of all commercially produced CSP systems is based on the assumption that such panels are treated as doubly reinforced sections without the consideration of the three-dimensional truss contribution of the orthotropic panel system. This leads to uneconomical design and underestimating both the strength and stiffness of such system. In this study, two of the most common types of commercially produced sandwich were evaluated both numerically and experimentally and results were used as basis for developing a genetic algorithm optimization process using numerical modeling simulations. In order to develop a sandwich panel with high structural performance, design optimization techniques are needed to achieve higher composite action, while maintaining the favorable features of such panels such as lightweight and high thermal insulation. The study involves both linear and nonlinear finite element analyses and parametric optimization. The verification and calibration of the numerical models is based on full-scale experimental results that were performed on two types of commercially produced sandwich panels under different loading scenarios. The genetic algorithm technique is used for optimization to identify an optimum design of the cementitious composite sandwich panels. The GA technique combines Darwin’s principle of survival of fittest and a structured information exchange using randomized crossover operators to evolve an optimum design for the cementitious sandwich panel. Parameters evaluated in the study include: (i) shear connectors’ geometry, its volume fraction and distribution; (ii) exterior cementitious face sheets thickness and (iii) size and geometry steel wires reinforcements. The proposed optimization method succeeded in reducing cost of materials of CSP by about 48% using genetic algorithm methodology. In addition, an optimized design for CSP is proposed that resulted in increasing the panel’s thermal resistance by 40% as compared to existing panels, while meeting ACI Code structural design criteria. Pareto-optimal front and Pareto-optimal solutions have been identified. Correlation between the design variables is also verified and design recommendation are proposed.
A Critical Review of Experimental Investigations about Convective Heat Transfer Characteristics of Nanofluids under Turbulent and Laminar Regimes with a Focus on the Experimental Setup
Authors: Colangelo Gianpiero , Diamante Noemi Francesca , Milanese Marco , Starace Giuseppe , de Risi Arturo
Published: September 2021

In this study, several experimental investigations on the effects of nanofluids on the convective heat transfer coefficient in laminar and turbulent conditions were analyzed. The aim of this work is to provide an overview of the thermal performance achieved with the use of nanofluids in various experimental systems. This review covers both forced and natural convection phenomena, with a focus on the different experimental setups used to carry out the experimental campaigns. When possible, a comparison was performed between different experimental campaigns to provide an analysis of the possible common points and differences. A significant increase in the convective heat transfer coefficient was found by using nanofluids instead of traditional heat transfer fluids, in general, even with big data dispersion from one case to another that depended on boundary conditions and the particular experimental setup. In particular, a general trend shows that once a critic value of the Reynolds number or nanoparticle concentrations is reached, the heat transfer performance of the nanofluid decreases or has no appreciable improvement. As a research field still under development, nanofluids are expected to achieve even higher performance and their use will be crucial in many industrial and civil sectors to increase energy efficiency and, thus, mitigate the environmental impact.
The Scientific Basis of the Target Plant Concept: An Overview
Authors: Davis Anthony S. , Pinto Jeremiah R.
Published: September 2021

Reforestation and restoration using nursery-produced seedlings is often the most reliable way to ensure successful establishment and rapid growth of native plants. Plant establishment success—that is, the ability for the plant to develop within a set period of time with minimal further interventions needed—depends greatly on decisions made prior to planting, and yet nursery-grown plants are often produced independently of considering the range of stressors encountered after nursery production. The optimal plant or seedling will vary greatly with species and site (depending on edaphic and environmental conditions), and in having the biological capacity to withstand human and wildlife pressures placed upon vegetative communities. However, when nursery production strategies incorporate knowledge of genetic variability, address limiting factors, and include potential mitigating measures, meeting the objectives of the planting project—be it reforestation or restoration—becomes more likely. The Target Plant Concept (TPC) is an effective framework for defining, producing, and handling seedlings and other types of plant material based on specific characteristics suited to a given site. These characteristics are often scientifically derived from testing factors that are linked to outplanting success, such as seedling morphology and physiology, genetic source, and capacity to overcome limiting factors on outplanting sites. This article briefly summarizes the current knowledge drawn from existing literature for each component of the TPC framework, thereby helping land managers and scientists to meet objectives and accelerate reforestation and restoration trajectories.
The Scaling of Blood Pressure and Volume
Authors: Chaui-Berlinck José Guilherme , Bicudo José Eduardo Pereira Wilken
Published: September 2021

The cardiovascular system is an apparatus of mass convection, and changes in organismic size impart changes in variables of this system, namely scaling effects. Blood flow depends on pressure and conductance, and the maintenance of flow results in entropy production, that is, loss of available work. In terms of scaling, it is well known that blood pressure is kept constant while blood volume varies linearly with body mass. Yet, such expected rules have never been proven. The present study shows that these scaling rules derive from the simultaneous optimization of blood flow and entropy production in circulation and how these impact the transition from ecto- to endotermy. Thus, for the first time in almost a century of data collection, these observed relationships are explained from a theoretical standpoint. The demonstration presented herein is a building block to form a solid basis for the other scaling rules of the cardiovascular system as well as of other organic systems. The approach is of wide interest in any area where generalized flow is analyzed in terms of system optimization, giving a broad perspective on change in either engineered or naturally evolving systems.
A Note on Existence of Mild Solutions for Second-Order Neutral Integro-Differential Evolution Equations with State-Dependent Delay
Authors: Rezapour Shahram , Henríquez Hernán R. , Vijayakumar Velusamy , Nisar Kottakkaran Sooppy , Shukla Anurag
Published: September 2021

This article is mainly devoted to the study of the existence of solutions for second-order abstract non-autonomous integro-differential evolution equations with infinite state-dependent delay. In the first part, we are concerned with second-order abstract non-autonomous integro-differential retarded functional differential equations with infinite state-dependent delay. In the second part, we extend our results to study the second-order abstract neutral integro-differential evolution equations with state-dependent delay. Our results are established using properties of the resolvent operator corresponding to the second-order abstract non-autonomous integro-differential equation and fixed point theorems. Finally, an application is presented to illustrate the theory obtained.
On the Application of Electron Energy-Loss Spectroscopy for Investigating Nanostructure of Soot from Different Fuels
Authors: Russo Carmela , Pawlyta Miroslawa , Tomiczek Anna Ewa , Ciajolo Anna , Apicella Barbara
Published: September 2021

Soot is characterized by a multiscale structural organization; the only diagnostic tool that can give access to it is the transmission electron microscope (TEM). However, as it is a diffraction-based technique, TEM images only conjugate aromatic systems and, thus, it is particularly useful to combine it with electron energy-loss spectroscopy (EELS), which is able to provide quantitative information about the relative abundance of sp3 and sp2 hybridized carbon. In this paper, a method for the EELS spectrum analysis of carbonaceous materials, recently developed for electron-irradiated graphite and glassy carbon composition analysis, has been applied for the first time on soot samples, in order to test its performance in soot nanostructure study in combination with TEM and high-resolution TEM (HRTEM). Soot samples analyzed were collected in the soot inception region of premixed flames of different hydrocarbon fuels. EELS, in agreement with TEM and HRTEM, showed a quite disordered and heterogeneous structure for young soot, with a relatively low sp2 content and slight presence of fullerene-like structures, more evident in the case of methane soot hinting to the effect of more saturated aliphatic fuels on soot characteristics at soot inception.
Introduction to the Special Issue “Pro-Sociality and Cooperation”
Authors: Ghidoni Riccardo
Published: September 2021

This short piece presents the contributions of the special issue of Games, “Pro-sociality and Cooperation” [...]
Impact of 18F-FDG PET/CT in Predicting Recurrence in Neurolymphomatosis
Authors: Uemichi Megumi , Yokoyama Kota , Tsuchiya Junichi , Terao Toshiki , Machida Youichi , Matsue Kosei , Tateishi Ukihide
Published: September 2021

To clarify the prognostic value of 2-[18F]fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography (18F-FDG PET/CT) in Neurolymphomatosis (NL), we retrospectively reviewed medical records of all NL patients who had undergone 18F-FDG PET/CT from 2007 to 2020 at Kameda Medical Center and Tokyo Medical and Dental University Hospital. The clinical data of patients were compared with 18F-FDG PET/CT findings of number of nerve lesions and presence of non-nerve extranodal lesions (ENL). Subsequently, we calculated recurrence-free survival (RFS) and overall survival (OS) using the Kaplan–Meier method. A total of 28 patients (mean age 70.1 years, range 44–87 years; 15 women) were included in the study and 7 patients (25.0%) relapsed NL. The number of nerve lesions detected by 18F-FDG PET/CT ranged from 1 to 5, with an average of 2.02. ENL was observed in 18 cases (64.3%). The comparison between the findings revealed that the more the lesions detected by 18F-FDG PET/CT, the higher the probability of recurrence (χ2 = 13.651, p = 0.0085) and there was significantly shorter RFS for the patients with 3 or more nerve lesions (p = 0.0059), whereas the presence of ENL was not significantly associated with any clinical findings. The present study revealed that the more nerve lesions detected by 18F-FDG PET/CT, the poorer the recurrence rate and RFS.
Multi-Scale Characterization of Unusual Green and Blue Pigments from the Pharaonic Town of Amara West, Nubia
Authors: Fulcher Kate , Siddall Ruth , Emmett Trevor F. , Spencer Neal
Published: September 2021

Pigments from paint palettes and a grindstone excavated from the pharaonic town of Amara West (c. 1300–1050 BCE), which lies between the Second and Third Cataracts of the Nile, were examined using polarized light microscopy, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction, and scanning electron microscopy with energy dispersive X-ray spectroscopy. Most of the pigments were consistent with the typical ancient Egyptian palette, but the greens and some blues were unusual. Two types of green pigment were identified, chlorite (varieties clinochlore and penninite) and copper chloride hydroxide (atacamite type). The former constitutes a type of green earth which has only rarely been identified in pharaonic Egyptian contexts and may be more widespread than is currently reported. The majority of the blue pigment samples were Egyptian blue, but some were found to be a blue earth, the main component of which being sodic amphibole riebeckite. The use of this mineral as a pigment has not previously been reported in any Nile Valley context. These results prompt questions around local and potentially indigenous practices within an ancient colonial context, and highlight avenues for future research.
Service Accessibility Risk (SAR) Assessment for Pluvial and Fluvial Floods in an Urban Context
Authors: Arosio Marcello , Arrighi Chiara , Cesarini Luigi , Martina Mario L. V.
Published: September 2021

The development of strategies to adapt to and mitigate the potential adverse consequences of natural hazards requires support from risk assessment studies that quantify the impacts of hazardous events on our society. A comprehensive analysis of risk commonly evaluates the elements exposed to the hazard probabilistic scenarios and their vulnerabilities. However, while significant advances have been made in the assessment of direct losses, indirect impacts are less frequently examined. This work assesses the indirect consequences of two hydrologic hazards, i.e., pluvial and fluvial floods, in an urban context from a system perspective. It presents a methodology to estimate the services accessibility risk (SAR) that considers the accessibility of roads and the connection between providers and users of services in a city. The feasibility of the proposed approach is illustrated by an application to a pilot study in Monza city (northern Italy) considering pluvial and fluvial flood hazard with different return periods. The results in terms of the social and economic impacts are analyzed considering features of age, disability, and the different economic sectors.
An Exploration of Domain-Specific Sedentary Behaviors in College Students by Lifestyle Factors and Sociodemographics
Authors: Carpenter Chelsea , Byun Sang-Eun , Turner-McGrievy Gabrielle , West Delia
Published: September 2021

College students exhibit high levels of sedentary time and/or poor lifestyle factors (e.g., poor sleep, stress, physical inactivity). It is unknown; however, in what domains college students spend their sedentary time and whether there are associations between sedentary time and these lifestyle factors. This study examined sedentary behavior of college students by domains, current lifestyle factors and sociodemographics. Undergraduates (n = 272, M age = 20 years, 79% female) self-reported their sedentary behavior, sleep, stress, physical activity, anthropometrics and sociodemographics. Sedentary time was categorized as: total, recreational screen, education and social. Students reported spending > 12 h of their day sedentary on average, with over a third of this time spent in recreational screen time. All categories of sedentary time were significantly correlated with body mass index, and both total sedentary time and screen time were significantly correlated with sleep score, with poorer sleep quality associated with greater sedentary time. Physical activity was negatively correlated with social sedentary time only. Subgroups with elevated sedentary time included minority students, those with low parental education and students with overweight/obesity. Given the negative health impacts of sedentary behavior, college students would likely benefit from interventions tailored to this population which target reducing sedentary time, particularly recreational screen time.
Robots in Healthcare? What Patients Say
Authors: Vallès-Peris Núria , Barat-Auleda Oriol , Domènech Miquel
Published: September 2021

In this paper, we analyse patients’ perspectives on the introduction of artificial intelligence (AI) and robotic systems in healthcare. Based on citizens’ experiences when hospitalised for COVID-19, we explore how the opinions and concerns regarding healthcare automation could not be disassociated from a context of high pressure on the health system and lack of resources, and a political discourse on AI and robotics; a situation intensified by the pandemic. Thus, through the analysis of a set of interviews, a series of issues are identified that revolve around the following: the empirical effects of imagined robots, the vivid experience of citizens with the care crisis, the discomfort of the ineffective, the virtualised care assemblages, the human-based face-to-face relationships, and the automatisation of healthcare tasks. In light of these results, we show the variability in patients’ perspectives on AI and robotic systems and explain it by distinguishing two interpretive repertoires that account for different views and opinions: a well-being repertoire and a responsibility repertoire. Both interpretative repertoires are relevant in order to grasp the complexity of citizens’ approaches to automatisation of healthcare. Attending to both allows us to move beyond the dominant (political) discourse of technology markets as the only way to respond to healthcare challenges. Thus, we can analyse and integrate patients’ perspectives to develop AI and robotic systems in healthcare to serve citizens’ needs and collective well-being.
Salt-Tolerant Compatible Microbial Inoculants Modulate Physio-Biochemical Responses Enhance Plant Growth, Zn Biofortification and Yield of Wheat Grown in Saline-Sodic Soil
Authors: Singh Udai B. , Malviya Deepti , Singh Shailendra , Singh Prakash , Ghatak Abhijeet , Imran Muhammad , Rai Jai P. , Singh Rajiv K. , Manna Madhab C. , Sharma Arun K. , Saxena Anil K.
Published: September 2021

A wide range of root-associated mutualistic microorganisms have been successfully applied and documented in the past for growth promotion, biofertilization, biofortification and biotic and abiotic stress amelioration in major crops. These microorganisms include nitrogen fixers, nutrient mobilizers, bio-remediators and bio-control agents. The present study aimed to demonstrate the impact of salt-tolerant compatible microbial inoculants on plant growth; Zn biofortification and yield of wheat (Triticum aestivum L.) crops grown in saline-sodic soil and insight of the mechanisms involved therein are being shared through this paper. Field experiments were conducted to evaluate the effects of Trichoderma harzianum UBSTH-501 and Bacillus amyloliquefaciens B-16 on wheat grown in saline-sodic soil at Research Farm, ICAR-Indian Institute of Seed Sciences, Kushmaur, India. The population of rhizosphere-associated microorganisms changed dramatically upon inoculation of the test microbes in the wheat rhizosphere. The co-inoculation induced a significant accumulation of proline and total soluble sugar in wheat at 30, 60, 90 and 120 days after sowing as compared to the uninoculated control. Upon quantitative estimation of organic solutes and antioxidant enzymes, these were found to have increased significantly in co-inoculated plants under salt-stressed conditions. The application of microbial inoculants enhanced the salt tolerance level significantly in wheat plants grown in saline-sodic soil. A significant increase in the uptake and translocation of potassium (K+) and calcium (Ca2+) was observed in wheat co-inoculated with the microbial inoculants, while a significant reduction in sodium (Na+) content was recorded in plants treated with both the bio-agents when compared with the respective uninoculated control plants. Results clearly indicated that significantly higher expression of TaHKT-1 and TaNHX1 in the roots enhances salt tolerance effectively by maintaining the Na+/K+ balance in the plant tissue. It was also observed that co-inoculation of the test inoculants increased the expression of ZIP transporters (2–3.5-folds) which ultimately led to increased biofortification of Zn in wheat grown in saline-sodic soil. Results suggested that co-inoculation of T. harzianum UBSTH-501 and B. amyloliquefaciens B-16 not only increased plant growth but also improved total grain yield along with a reduction in seedling mortality in the early stages of crop growth. In general, the present investigation demonstrated the feasibility of using salt-tolerant rhizosphere microbes for plant growth promotion and provides insights into plant-microbe interactions to ameliorate salt stress and increase Zn bio-fortification in wheat.
Parental Monitoring, Individual Dispositions, and Alcohol Use Disorder: A Longitudinal Study with Young Swiss Men
Authors: Sobrino-Piazza Jimena , Foster Simon , Estévez-Lamorte Natalia , Mohler-Kuo Meichun
Published: September 2021

This paper evaluates the interaction between level of parental monitoring in adolescence and individual dispositions present in early adulthood in the prediction of alcohol use disorder (AUD) in the mid-20s. Data were drawn from the Cohort Study on Substance Use Risk Factors (C-SURF), encompassing 4844 young Swiss men who were surveyed three times within a 5-year period. The outcome variable was alcohol use disorder (AUD) as defined in the DSM-5. Independent variables were sensation seeking (Brief Sensation Seeking Scale) and the coping strategies active coping and denial (Brief COPE). Low parental monitoring, high sensation seeking, and high denial were found risk factors of AUD (odds ratio (OR) = 1.21 (1.05–1.40); OR = 1.56 (1.37–1.78); OR = 1.15 (1.01–1.31)). A significant interaction effect was identified between active coping and parental monitoring; high active coping in early adulthood was found protective of AUD, only among individuals who had low parental monitoring in adolescence (OR = 0.70 (0.52–0.96)). In addition to interventions to upskill parents for improving monitoring, other interventions directed to young adults who had disadvantaged family contexts could be implemented, with the aim of enhancing the use of adaptive coping strategies such as active coping. Prevention targeting avoidant coping strategies and sensation seeking should be privileged too.
Air Pollutant Particles, PM2.5, Exposure and Glaucoma in Patients with Diabetes: A National Population-Based Nested Case–Control Study
Authors: Chiang Yun-Wei , Wu Sheng-Wen , Luo Ci-Wen , Chen Shih-Pin , Chen Chun-Jung , Chen Wen-Ying , Chang Chia-Che , Chen Chuan-Mu , Kuan Yu-Hsiang
Published: September 2021

The global prevalence of diabetes mellitus (DM) has reached 20%. Air pollutants with a particle size of less than 2.5 μm (PM2.5) are a globally recognized risk factor for diabetes and glaucoma. We examined whether the risk of glaucoma would decrease or increase when patients with DM were exposed to different PM2.5 concentrations. Data were obtained from the National Health Insurance Research Database (NHIRD) of Taiwan and the Air Quality Monitoring Network between 2008 and 2013. This nested case–control study involved 197 DM patients with glaucoma and 788 DM patients without glaucoma. Cases and controls were matched (1:4) by gender, age (±5 years), and index date (±6 months), and their data were entered in a logistic regression model adjusted for gender, age, urbanization level, income level, and comorbidities. The odds ratio (OR) of glaucoma at PM2.5 exposure concentration in the fourth quartile (Q4) compared with in the first quartile (Q1) was 1.7 (95% CI: 1.084–2.764). For glaucoma risk, the OR was 1.013 (95% CI: 1.006–1.020) at a PM2.5 exposure concentration in Q1, 1.004 (95% CI: 1.001–1.007) in the third quartile (Q3), and 1.003 (95% CI: 1.001–1.004) in Q4. In the subgroup analysis of patients living in non-emerging towns and non-agricultural towns, the OR for glaucoma in Q4 compared with in Q1 was 2.1 (95% CI: 1.229–3.406) and 1.8 (95% CI: 1.091–2.803), respectively (p trend = 0.001 and 0.011). For patients without migraine, the OR for glaucoma was 1.7 (95% CI: 1.074–2.782; p = 0.006). These results demonstrate that, for patients with DM, PM2.5 increased the risk of glaucoma and PM2.5 was an independent risk factor for glaucoma in patients with DM.
Do Deposit Insurance Systems Promote Banking Stability?
Authors: Alam Nafis , Sivarajah Ganesh , Bhatti Muhammad Ishaq
Published: September 2021

During the global financial crisis (GFC), regulators and policymakers turned to deposit insurers, along with monetary and fiscal measures, to help restore market confidence and promote financial stability. These events have focused attention on the role of deposit insurers and their role in the banking system. Recent literature reveals that during the GFC, deposit insurance maintained banking stability and successfully prevented customers doing ‘runs’ on the banks. The objective of this paper is to examine the deposit insurance system’s coverage limits and the impact on banking stability, in the context of a jurisdiction’s economic and institutional environment. Our model examines 61 jurisdictions in Asia and Europe with explicit deposit insurance systems, covering the pre- and post-GFC period between 2004 and 2014. We also examine subsets to investigate the effects of the region by comparing Asia and Europe, as well as a subset using the date of establishment of the deposit insurance system to understand if maturity matters. The results indicate that deposit insurance systems, and specifically deposit insurance coverage levels, have both positive and negative effects on banking stability. We find significant associations with certain economic and institutional factors; however, there are differences between the models we ran. These can be ascribed to regional factors and the date of when a deposit insurance system was established.
The Osteogenic Differentiation of Human Dental Pulp Stem Cells through G0/G1 Arrest and the p-ERK/Runx-2 Pathway by Sonic Vibration
Authors: Lee Won , Eo Su-Rak , Choi Ju-Hye , Kim Yu-Mi , Nam Myeong-Hyun , Seo Young-Kwon
Published: September 2021

Mechanical/physical stimulations modulate tissue metabolism, and this process involves multiple cellular mechanisms, including the secretion of growth factors and the activation of mechano-physically sensitive kinases. Cells and tissue can be modulated through specific vibration-induced changes in cell activity, which depend on the vibration frequency and occur via differential gene expression. However, there are few reports about the effects of medium-magnitude (1.12 g) sonic vibration on the osteogenic differentiation of human dental pulp stem cells (HDPSCs). In this study, we investigated whether medium-magnitude (1.12 g) sonic vibration with a frequency of 30, 45, or 100 Hz could affect the osteogenic differentiation of HDPSCs. Their cell morphology changed to a cuboidal shape at 45 Hz and 100 Hz, but the cells in the other groups were elongated. FACS analysis showed decreased CD 73, CD 90, and CD 105 expression at 45 Hz and 100 Hz. Additionally, the proportions of cells in the G0/G1 phase in the control, 30 Hz, 45 Hz, and 100 Hz groups after vibration were 60.7%, 65.9%, 68.3%, and 66.7%, respectively. The mRNA levels of osteogenic-specific markers, including osteonectin, osteocalcin, BMP-2, ALP, and Runx-2, increased at 45 and 100 Hz, and the ALP and calcium content was elevated in the vibration groups compared with those in the control. Additionally, the western blotting results showed that p-ERK, BSP, osteoprotegerin, and osteonectin proteins were upregulated at 45 Hz compared with the other groups. The vibration groups showed higher ALP and calcium content than the control. Vibration, especially at 100 Hz, increased the number of calcified nodes relative to the control group, as evidenced by von Kossa staining. Immunohistochemical staining demonstrated that type I and III collagen, osteonectin, and osteopontin were upregulated at 45 Hz and 100 Hz. These results suggest that medium magnitude vibration at 45 Hz induces the G0/G1 arrest of HDPSCs through the p-ERK/Runx-2 pathway and can serve as a potent stimulator of differentiation and extracellular matrix production.
“Take It or Leave It”—Factors Regulating Competence Development and DNA Uptake in Campylobacter jejuni
Authors: Golz Julia C. , Stingl Kerstin
Published: September 2021

Campylobacter jejuni has a large adaptive potential due to enormous genetic exchange. Factors regulating natural transformation in this food-borne pathogen are largely unknown but of interest for the application of sustained reduction strategies in the food-processing industry. Using a single cell DNA uptake assay, we visualized that recognition of methylated C. jejuni DNA was essential for the first step of DNA uptake into a DNase resistant state. Transformation rates using a resistance marker correlated with the fraction of competent bacteria, harboring one to maximally four locations of active DNA uptake, not necessarily being located at the cell pole. Competence developed with rising pH between 6.5 and 7.5 under microaerobic conditions and was nearly insensitive towards growth temperatures between 32 °C and 42 °C, CO2 concentrations ranging from 0 to 50% and growth rates. However, competence development was abolished at pH 5 or under aerobic stress conditions, in which the bacteria ceased growth but fully survived. The DNA uptake machinery in competent bacteria shut down at slightly acidic pH and was reversibly switched on upon neutralization. It was dependent on the proton motive force and, in contrast to competence development, slightly enhanced under aerobic conditions. The results suggest that natural transformation in C. jejuni occurs in the neutral and microaerobic intestinal environment for enhanced genetic diversity and pre-adaption before host switch. In addition, highly competent bacteria might be shed into the environment, still able to acquire genetic material for increased survival.
Influence of Ce3+ Substitution on Antimicrobial and Antibiofilm Properties of ZnCexFe2−xO4 Nanoparticles (X = 0.0, 0.02, 0.04, 0.06, and 0.08) Conjugated with Ebselen and Its Role Subsidised with γ-Radiation in Mitigating Human TNBC and Colorectal Adenocarcinoma Proliferation In Vitro
Authors: Abdel-Rafei Mohamed K. , Thabet Noura M. , Abdel Maksoud M. I. A. , Abd Elkodous M. , Kawamura Go , Matsuda Atsunori , Ashour A. H. , El-Batal Ahmed I. , El-Sayyad Gharieb S.
Published: September 2021

Cancers are a major challenge to health worldwide. Spinel ferrites have attracted attention due to their broad theranostic applications. This study aimed at investigating the antimicrobial, antibiofilm, and anticancer activities of ebselen (Eb) and cerium-nanoparticles (Ce-NPs) in the form of ZnCexFe2−XO4 on human breast and colon cancer cell lines. Bioassays of the cytotoxic concentrations of Eb and ZnCexFe2−XO4, oxidative stress and inflammatory milieu, autophagy, apoptosis, related signalling effectors, the distribution of cells through the cell-cycle phases, and the percentage of cells with apoptosis were evaluated in cancer cell lines. Additionally, the antimicrobial and antibiofilm potential have been investigated against different pathogenic microbes. The ZOI, and MIC results indicated that ZnCexFe2−XO4; X = 0.06 specimen reduced the activity of a wide range of bacteria and unicellular fungi at low concentration including P. aeruginosa (9.5 mm; 6.250 µg/mL), S. aureus (13.2 mm; 0.390 µg/mL), and Candida albicans (13.5 mm; 0.195 µg/mL). Reaction mechanism determination indicated that after ZnCexFe2−xO4; X = 0.06 treatment, morphological differences in S.aureus were apparent with complete lysis of bacterial cells, a concomitant decrease in the viable number, and the growth of biofilm was inhibited. The combination of Eb with ZFO or ZnCexFe2−XO4 with γ-radiation exposure showed marked anti-proliferative efficacy in both cell lines, through modulating the oxidant/antioxidant machinery imbalance, restoring the fine-tuning of redox status, and promoting an anti-inflammatory milieu to prevent cancer progression, which may be a valuable therapeutic approach to cancer therapy and as a promising antimicrobial agent to reduce the pathogenic potential of the invading microbes.
Dynamic Crosstalk between Vascular Smooth Muscle Cells and the Aged Extracellular Matrix
Authors: Ribeiro-Silva Joao Carlos , Nolasco Patricia , Krieger Jose Eduardo , Miyakawa Ayumi Aurea
Published: September 2021

Vascular aging is accompanied by the fragmentation of elastic fibers and collagen deposition, leading to reduced distensibility and increased vascular stiffness. A rigid artery facilitates elastin to degradation by MMPs, exposing vascular cells to greater mechanical stress and triggering signaling mechanisms that only exacerbate aging, creating a self-sustaining inflammatory environment that also promotes vascular calcification. In this review, we highlight the role of crosstalk between smooth muscle cells and the vascular extracellular matrix (ECM) and how aging promotes smooth muscle cell phenotypes that ultimately lead to mechanical impairment of aging arteries. Understanding the underlying mechanisms and the role of associated changes in ECM during aging may contribute to new approaches to prevent or delay arterial aging and the onset of cardiovascular diseases.
Blockade of Serotonin 5-HT6 Receptor Constitutive Activity Alleviates Cognitive Deficits in a Preclinical Model of Neurofibromatosis Type 1
Authors: Doucet Emilie , Grychowska Katarzyna , Zajdel Pawel , Bockaert Joël , Marin Philippe , Bécamel Carine
Published: September 2021

Neurofibromatosis type 1 (NF1) is a common inherited disorder caused by mutations of the NF1 gene that encodes the Ras-GTPase activating protein neurofibromin, leading to overactivation of Ras-dependent signaling pathways such as the mTOR pathway. It is often characterized by a broad range of cognitive symptoms that are currently untreated. The serotonin 5-HT6 receptor is a potentially relevant target in view of its ability to associate with neurofibromin and to engage the mTOR pathway to compromise cognition in several cognitive impairment paradigms. Here, we show that constitutively active 5-HT6 receptors contribute to increased mTOR activity in the brain of Nf1+/− mice, a preclinical model recapitulating some behavioral alterations of NF1. Correspondingly, peripheral administration of SB258585, a 5-HT6 receptor inverse agonist, or rapamycin, abolished deficits in long-term social and associative memories in Nf1+/− mice, whereas administration of CPPQ, a neutral antagonist, did not produce cognitive improvement. These results show a key influence of mTOR activation by constitutively active 5-HT6 receptors in NF1 cognitive symptoms. They provide a proof of concept that 5-HT6 receptor inverse agonists already in clinical development as symptomatic treatments to reduce cognitive decline in dementia and psychoses, might be repurposed as therapies alleviating cognitive deficits in NF1 patients.
Detection and Evaluation of Serological Biomarkers to Predict Osteoarthritis in Anterior Cruciate Ligament Transection Combined Medial Meniscectomy Rat Model
Authors: Huang Nian-Cih , Yang Tsorng-Shyang , Busa Prabhakar , Lin Ching-Ling , Fang Ya-Chieh , Chen Ing-Jung , Wong Chih-Shung
Published: September 2021

Biomarkers are essential tools in osteoarthritis (OA) research, clinical trials, and drug development. Detecting and evaluating biomarkers in OA research can open new avenues for researching and developing new therapeutics. In the present report, we have explored the serological detection of various osteoarthritis-related biomarkers in the preclinical model of OA. In this surgical OA model, we disrupted the medial tibial cartilage’s integrity via anterior cruciate ligament transection combined with medial meniscectomy (ACLT+MMx) of a single joint of Wistar rats. The progression of OA was verified, as shown by the microscopic deterioration of cartilage and the increasing cartilage degeneration scoring from 4 to 12 weeks postsurgery. The concentration of serological biomarkers was measured at two timepoints, along with the complete blood count and bone electrolytes, with biochemical analysis further conducted. The panel evaluated inflammatory biomarkers, bone/cartilage biomarkers, and lipid metabolic pathway biomarkers. In chronic OA rats, we found a significant reduction of total vitamin D3 and C-telopeptide fragments of type II (CTX-II) levels in the serum as compared to sham-operated rats. In contrast, the serological levels of adiponectin, leptin, and matrix metallopeptidase (MMP3) were significantly enhanced in chronic OA rats. The inflammatory markers, blood cell composition, and biochemical profile remained unchanged after surgery. In conclusion, we found that a preclinical model of single-joint OA with significant deterioration of the cartilage can lead to serological changes to the cartilage and metabolic-related biomarkers without alteration of the systemic blood and biochemical profile. Thus, this biomarker profile provides a new tool for diagnostic/therapeutic assessment in OA scientific research.
The Editor’s Choice for Issue 2, Volume 7
Authors: Ficicioglu Can
Published: September 2021

Dear readers: I am proud to say that we are continuing to publish many important papers on newborn screening in IJNS, and the papers published in this issue clearly support my statement [...]
Interleukin-6 Trans-Signaling Mediated Regulation of Paracellular Permeability in Human Retinal Endothelial Cells
Authors: Glass Joshua , Robinson Rebekah , Lee Tae-Jin , Sharma Ashok , Sharma Shruti
Published: September 2021

Long-term hyperglycemia-mediated oxidative stress and inflammation lead to the blood-retinal barrier (BRB) dysfunction and increased vascular permeability associated with diabetic retinopathy (DR). Interleukin-6 (IL-6) is one of the primary mediators of retinal vascular inflammation. IL-6 signaling through its membrane-bound IL-6 receptor is known as classical signaling, and through a soluble IL-6 receptor (sIL-6R) is known as trans-signaling. Increasing evidence suggests that classical signaling is primarily anti-inflammatory, whereas trans-signaling induces the pro-inflammatory effects of IL-6. The purpose of this study was to compare the effects of these two pathways on paracellular permeability and expression of genes involved in inter-endothelial junctions in human retinal endothelial cells (HRECs). IL-6 trans-signaling activation caused significant disruption to paracellular integrity, with increased paracellular permeability, and was associated with significant changes in gene expression related to adherens, tight, and gap junctions. IL-6 classical signaling did not alter paracellular resistance in HRECs and had no distinct effects on gene expression. In conclusion, IL-6 trans-signaling, but not classical signaling, is a major mediator of the increased paracellular permeability characteristic of inner BRB breakdown in diabetic retinopathy. This study also identified potential inter-endothelial junction genes involved in the IL-6 trans-signaling mediated regulation of paracellular permeability in HRECs.
Diagnosis of Subclinical Keratoconus Based on Machine Learning Techniques
Authors: Castro-Luna Gracia , Jiménez-Rodríguez Diana , Castaño-Fernández Ana Belén , Pérez-Rueda Antonio
Published: September 2021

(1) Background: Keratoconus is a non-inflammatory corneal disease characterized by gradual thinning of the stroma, resulting in irreversible visual quality and quantity decline. Early detection of keratoconus and subsequent prevention of possible risks are crucial factors in its progression. Random forest is a machine learning technique for classification based on the construction of thousands of decision trees. The aim of this study was to use the random forest technique in the classification and prediction of subclinical keratoconus, considering the metrics proposed by Pentacam and Corvis. (2) Methods: The design was a retrospective cross-sectional study. A total of 81 eyes of 81 patients were enrolled: sixty-one eyes with healthy corneas and twenty patients with subclinical keratoconus (SCKC): This initial stage includes patients with the following conditions: (1) minor topographic signs of keratoconus and suspicious topographic findings (mild asymmetric bow tie, with or without deviation; (2) average K (mean corneal curvature) < 46, 5 D; (3) minimum corneal thickness (ECM) > 490 μm; (4) no slit lamp found; and (5) contralateral clinical keratoconus of the eye. Pentacam topographic and Corvis biomechanical variables were collected. Decision tree and random forest were used as machine learning techniques for classifications. Random forest performed a ranking of the most critical variables in classification. (3) Results: The essential variable was SP A1 (stiffness parameter A1), followed by A2 time, posterior coma 0°, A2 velocity and peak distance. The model efficiently predicted all patients with subclinical keratoconus (Sp = 93%) and was also a good model for classifying healthy cases (Sen = 86%). The overall accuracy rate of the model was 89%. (4) Conclusions: The random forest model was a good model for classifying subclinical keratoconus. The SP A1 variable was the most critical determinant in classifying and identifying subclinical keratoconus, followed by A2 time.
Do Women Have a Higher Mortality Risk Than Men following ICU Admission after Out-of-Hospital Cardiac Arrest? A Retrospective Cohort Analysis
Authors: van Wees Christiaan , Rietdijk Wim , Mandigers Loes , van der Graaf Marisa , Scholte Niels T. B. , Adriaansens Karst O. , van den Berg Remco C. M. , den Uil Corstiaan A.
Published: September 2021

Purpose: previous studies showed that women have a higher mortality risk than men after out-of-hospital cardiac arrest (OHCA). This sex difference may disappear after adjustment for cardiac arrest characteristics. Most studies also included patients who were not admitted to the intensive care unit (ICU). We analyzed whether sex impacts the mortality of ICU-admitted OHCA patients. Methods: a retrospective cohort analysis of 1240 OHCA patients admitted to the ICU (310 women, 25%, AgeMedian 64.0 (IQR 53.8–73.0)) at an academic hospital in the Netherlands between 1 January 2007 and 31 December 2018. The primary outcome was 90-day mortality; the secondary outcome was a favorable cerebral performance category (CPC) score at ICU discharge and ICU length of stay (ICU LOS). Results: we found no association between sex and 90-day mortality (hazard ratio (HR) 0.867; 95% confidence interval (95% CI) 0.678–1.108) after adjusting for relevant cardiac arrest characteristics. Similarly, we found no difference for favorable CPC score (OR 1.117; 95% CI 0.777–1.608) or ICU LOS between sexes (Beta 0.428; 95% CI −0.442 to 1.298). Conclusions: after adjusting for cardiac arrest characteristics, we found no difference between women and men with respect to 90-day mortality, ICU LOS, and CPC score.