bluuebunny/crossref_metadata_2025 huggingface dataset:
An Application of a Hybrid Intelligent System for Diagnosing Primary Headaches
Authors: Simić Svetlana , Villar José R. , Calvo-Rolle José Luis , Sekulić Slobodan R. , Simić Svetislav D. , Simić Dragan
Published: February 2021

(1) Background: Modern medicine generates a great deal of information that stored in medical databases. Simultaneously, extracting useful knowledge and making scientific decisions for diagnosis and treatment of diseases becomes increasingly necessary. Headache disorders are the most prevalent of all the neurological conditions. Headaches have not only medical but also great socioeconomic significance. The aim of this research is to develop an intelligent system for diagnosing primary headache disorders. (2) Methods: This research applied various mathematical, statistical and artificial intelligence techniques, among which the most important are: Calinski-Harabasz index, Analytical Hierarchy Process, and Weighted Fuzzy C-means Clustering Algorithm. These methods, techniques and methodologies are used to create a hybrid intelligent system for diagnosing primary headache disorders. The proposed intelligent diagnostic system is tested with original real-world data set with different metrics. (3) Results: First at all, nine of 20 attributes – features from International Headache Society (IHS) criteria are selected, and then only five most important attributes from IHS criteria are selected. The calculation result based on the Calinski–Harabasz index value (178) for the optimal number of clusters is three, and they present three classes of headaches: (i) migraine, (ii) tension-type headaches (TTHs), and (iii) other primary headaches (OPHs). The proposed hybrid intelligent system shows the following quality metrics: Accuracy 75%; Precision 67% for migraine, 74% for TTHs, 86% for OPHs, and Average Precision 77%; Recall 86% for migraine, 73% for TTHs, 67% for OPHs, Average Recall 75%; F1 score 75% for migraine, 74% for TTHs, 75% for OPHs, and Average F1 score 75%. (4) Conclusions: The hybrid intelligent system presents qualitative and respectable experimental results. The implementation of existing diagnostics systems and the development of new diagnostics systems in medicine is necessary in order to help physicians make quality diagnosis and decide the best treatments for the patients.
The Role of Novel (Tobacco) Products on Tobacco Control in Italy
Authors: Gallus Silvano , Borroni Elisa , Odone Anna , van den Brandt Piet A. , Gorini Giuseppe , Spizzichino Lorenzo , Pacifici Roberta , Lugo Alessandra
Published: February 2021

In Italy, electronic cigarettes have spread since 2010 and heated tobacco products (HTP) since 2016. We investigated their public health consequences on conventional cigarette smoking, taking advantage of a series of cross-sectional studies annually conducted between 2001 and 2019 in Italy. Every year, the sample, including around 3000 individuals, was representative of the general Italian population aged ≥15 years. In Italy, smoking prevalence steadily declined from 29.1% in 2001 to 20.6% in 2013, then increased to 22.0% in 2019. In 2017–2019, current electronic cigarette users were 2.1% and in 2019 current HTP users were 1.1%. Among 498 ever electronic cigarette users, 23.2% started or re-started smoking and 15.7% quit smoking after electronic cigarette use; of 49 ever HTP users, 19.1% started or re-started smoking combusted cigarettes and 14.6% quit smoking after HTP use. The availability of novel products in Italy resulted in a halt of the decreasing trend in smoking prevalence. For the first time, we observed an increase of Italians inhaling nicotine, concurrently with the spread of novel (tobacco) products. More importantly, the use of novel products appears to increase—rather than decrease—the likelihood of smoking conventional cigarettes. Considering this evidence, we see no argument to justify the huge fiscal and regulatory benefits these products continue to have, at least in Italy.
Factors That Impact the Adoption of Clinical Decision Support Systems (CDSS) for Antibiotic Management
Authors: Laka Mah , Milazzo Adriana , Merlin Tracy
Published: February 2021

The study evaluated individual and setting-specific factors that moderate clinicians’ perception regarding use of clinical decision support systems (CDSS) for antibiotic management. A cross-sectional online survey examined clinicians’ perceptions about CDSS implementation for antibiotic management in Australia. Multivariable logistic regression determined the association between drivers of CDSS adoption and different moderators. Clinical experience, CDSS use and care setting were important predictors of clinicians’ perception concerning CDSS adoption. Compared to nonusers, CDSS users were less likely to lack confidence in CDSS (OR = 0.63, 95%, CI = 0.32, 0.94) and consider it a threat to professional autonomy (OR = 0.47, 95%, CI = 0.08, 0.83). Conversely, there was higher likelihood in experienced clinicians (>20 years) to distrust CDSS (OR = 1.58, 95%, CI = 1.08, 2.23) due to fear of comprising their clinical judgement (OR = 1.68, 95%, CI = 1.27, 2.85). In primary care, clinicians were more likely to perceive time constraints (OR = 1.96, 95%, CI = 1.04, 3.70) and patient preference (OR = 1.84, 95%, CI = 1.19, 2.78) as barriers to CDSS adoption for antibiotic prescribing. Our findings provide differentiated understanding of the CDSS implementation landscape by identifying different individual, organisational and system-level factors that influence system adoption. The individual and setting characteristics can help understand the variability in CDSS adoption for antibiotic management in different clinicians.
Air Pollution Health Risk Assessment (AP-HRA), Principles and Applications
Authors: Hassan Bhat Tavoos , Jiawen Guo , Farzaneh Hooman
Published: February 2021

Air pollution is a major public health problem. A significant number of epidemiological studies have found a correlation between air quality and a wide variety of adverse health impacts emphasizing a considerable role of air pollution in the disease burden in the general population ranging from subclinical effects to premature death. Health risk assessment of air quality can play a key role at individual and global health promotion and disease prevention levels. The Air Pollution Health Risk Assessment (AP-HRA) forecasts the expected health effect of policies impacting air quality under the various policy, environmental and socio-economic circumstances, making it a key tool for guiding public policy decisions. This paper presents the concept of AP-HRA and offers an outline for the proper conducting of AP-HRA for different scenarios, explaining in broad terms how the health hazards of air emissions and their origins are measured and how air pollution-related impacts are quantified. In this paper, seven widely used AP-HRA tools will be deeply explored, taking into account their spatial resolution, technological factors, pollutants addressed, geographical scale, quantified health effects, method of classification, and operational characteristics. Finally, a comparative analysis of the proposed tools will be conducted, using the SWOT (strengths, weaknesses, opportunities, and threats) method.
The Effectiveness of an Educational Intervention on Knowledge, Attitudes and Reported Practices on Antibiotic Use in Humans and Pigs: A Quasi-Experimental Study in Twelve Villages in Shandong Province, China
Authors: Shen Liyan , James Dyar Oliver , Sun Qiang , Wei Xiaolin , Yang Ding , Sun Chengtao , Wang Yang , Li Hongyu , Liu Yuqing , Luo Yanbo , Yin Jia , Stålsby Lundborg Cecilia
Published: February 2021

Our aim was to evaluate the effectiveness of an intervention for residents in rural China on knowledge, attitudes and reported practices (KAP) on antibiotic use in humans and pigs. A quasi-experimental study was conducted in 12 villages in rural Shandong province, divided into intervention and control groups, covering a two-year period from July 2015 to June 2017. A package of health education-based interventions including training sessions, speakerphone messages, posters and handbooks for residents was developed and implemented over a one-year period to improve the use of antibiotics in humans and pigs. The intervention net effects were evaluated by Difference-in-Difference (DID) analysis based on responses to a questionnaire concerning KAP towards antibiotic use in humans and pigs. A total of 629 participants completed both baseline and post-trial questionnaires, including 127 participants with backyard pig farms. Significant improvements were found in KAP towards antibiotic use in humans, but changes related to antibiotic use for pigs were not significant. Participants who were in the intervention group (p < 0.001) were more likely to have improved their knowledge on antibiotic use in humans. Participants who had higher attitude scores were less likely to report self-medicating with stored antibiotics in the previous year (p < 0.001). Our results suggest that our health education-based intervention was effective in improving KAP on human antibiotic use, but it had little effect regarding antibiotic use for pigs.
Qualitative and Quantitative Assessment of Buckwheat Husks as a Material for Use in Therapeutic Mattresses
Authors: Nawirska-Olszańska Agnieszka , Figiel Adam , Pląskowska Elżbieta , Twardowski Jacek , Gębarowska Elżbieta , Kucharska Alicja Z. , Sokół-Łętowska Anna , Spychaj Radosław , Lech Krzysztof , Liszewski Marek
Published: February 2021

Buckwheat husks are used in many therapeutic products such as pillows, mattresses, seats, etc. This material is proposed by producers for example for discopathy, back pain and head vasomotor disorders. Our studies evaluated the impact of using cotton mattresses with buckwheat husk fillings on people’s health condition. The main research was carried out on the group of 60 people divided into 3 groups (1—people with skeletal system problems, 2—people spending a lot of time lying with the probability of pressure ulcer formation and 3—healthy people). In addition, different tests have been carried out on the possibility of colonization of mattresses by fungi, bacteria and arthropod pests, and rheological, chemical and flammability tests. The research material in the form of buckwheat husks was tested in a diverse way. All tests indicate high usefulness of husks for therapeutic activity. This material was contaminated with fungi, bacteria and pests at a very low level, related to the natural colonization of buckwheat nuts during harvest and storage. The quality of the husks was also confirmed in rheological, chemical and flammability studies. Finally, this has also been confirmed in surveys conducted on people with health problems. The analyses show that the buckwheat husk is an excellent material that can be used to fill prophylactic mattresses. This has been confirmed by the results of laboratory tests and opinions of respondents using mattresses filled with buckwheat husk.
Comprehensive Risk Assessment of Schistosomiasis Epidemic Based on Precise Identification of Oncomelania hupensis Breeding Grounds—A Case Study of Dongting Lake Area
Authors: Xu Jun , Ouyang Xiao , He Qingyun , Wei Guoen
Published: February 2021

Spatio-temporal epidemic simulation, assessment, and risk monitoring serve as the core to establishing and improving the national public health emergency management system. In this study, we investigated Oncomelania hupensis breeding grounds and analyzed the locational and environmental preferences of snail breeding in Dongting Lake (DTL), Hunan, China. Using geographic information systems and remote sensing technology, we identified schistosomiasis risk areas and explored the factors affecting the occurrence and transmission of the disease. Several key conclusions were drawn. (1) From 2006 to 2016, the spatial change of potential O. hupensis breeding risk showed a diminishing trend from the eastern and northern regions to southwest DTL. Environmental changes in the eastern DTL region resulted in the lakeside and hydrophilic agglomerations of the O. hupensis populations. The shift in snail breeding grounds from a fragmented to centralized distribution indicates the weakening mobility of the O. hupensis population, the increasing independence of solitary groups, and the growing dependence of the snail population to the local environment. (2) The spatial risk distribution showed a descending gradient from west Dongting area to the east and an overall pattern of high in the periphery of large lakes and low in other areas. The cold-spot areas had their cores in Huarong County and Anxiang County and were scattered throughout the peripheral areas. The hot-spot areas had their center at Jinshi City, Nanxian County, and the southern part of Huarong County. The areas with increased comprehensive risks changed from centralized and large-scale development to fragmented shrinkage with increased partialization in the core area. The risk distribution’s center shifted to the northwest. The spatial risk distribution exhibited enhanced concentricity along the major axis and increased dispersion along the minor axis.
Melanoma Prevention: Comparison of Different Screening Methods for the Selection of a High Risk Population
Authors: Dubbini Nevio , Puddu Antonella , Salimbeni Grazia , Malloggi Stefano , Gandini Daniele , Massei Pietro , Ferraùto Giuseppe , Rubino Tommaso , Ricci Laura , Menchini Giovanni , Celli Marco , Ghilardi Maurizia , Gianfaldoni Roberto , Gianfaldoni Serena , Nannipieri Andrea , Romanini Antonella
Published: February 2021

Background: Guidelines recommend limiting melanoma screening in a population with known risk factors, but none indicates methods for efficient recruitment. The purpose of this study is to compare three different methods of recruiting subjects to be screened for melanoma to detect which, if any, is the most efficient. Methods: From 2010 to 2019, subjects were recruited as follows: (1) regular skin examinations (RS), mainly conducted through the Associazione Contro il Melanoma network; (2) occasional melanoma screening (OS), during annual public campaigns; (3) and selective screening (SS), where people were invited to undergo a skin check after filling in a risk evaluation questionnaire, in cases where the assigned outcome was intermediate/high risk. Melanoma risk factors were compared across different screening methods. Generalized Linear Mixed Models were used for multivariable analysis. Results: A total of 2238 subjects (62.7% women) were recruited, median age 44 years (2–85), and 1094 (48.9 %) records were collected through RS, 826 (36.9 %) through OS, and 318 (14.2 %) through SS. A total of 131 suspicious non-melanoma skin cancers were clinically diagnosed, 20 pathologically confirmed, and 2 melanomas detected. SS performed significantly better at selecting subjects with a family history of melanoma and I-II phototypes compared to OS. Conclusions: Prior evaluation of melanoma known risk factors allowed for effective selection of a population to screen at higher risk of developing a melanoma.
Mast Cell and Astrocyte Hemichannels and Their Role in Alzheimer’s Disease, ALS, and Harmful Stress Conditions
Authors: Harcha Paloma A. , Garcés Polett , Arredondo Cristian , Fernández Germán , Sáez Juan C. , van Zundert Brigitte
Published: February 2021

Considered relevant during allergy responses, numerous observations have also identified mast cells (MCs) as critical effectors during the progression and modulation of several neuroinflammatory conditions, including Alzheimer’s disease (AD) and amyotrophic lateral sclerosis (ALS). MC granules contain a plethora of constituents, including growth factors, cytokines, chemokines, and mitogen factors. The release of these bioactive substances from MCs occurs through distinct pathways that are initiated by the activation of specific plasma membrane receptors/channels. Here, we focus on hemichannels (HCs) formed by connexins (Cxs) and pannexins (Panxs) proteins, and we described their contribution to MC degranulation in AD, ALS, and harmful stress conditions. Cx/Panx HCs are also expressed by astrocytes and are likely involved in the release of critical toxic amounts of soluble factors—such as glutamate, adenosine triphosphate (ATP), complement component 3 derivate C3a, tumor necrosis factor (TNFα), apoliprotein E (ApoE), and certain miRNAs—known to play a role in the pathogenesis of AD, ALS, and other neurodegenerative disorders. We propose that blocking HCs on MCs and glial cells offers a promising novel strategy for ameliorating the progression of neurodegenerative diseases by reducing the release of cytokines and other pro-inflammatory compounds.
Effect of Hofmeister Ions on Transport Properties of Aqueous Solutions of Sodium Hyaluronate
Authors: Musilová Lenka , Mráček Aleš , Kašpárková Věra , Minařík Antonín , Valente Artur J. M. , Azevedo Eduarda F. G. , Veríssimo Luis M. P. , Rodrigo M. Melia , Esteso Miguel A. , Ribeiro Ana C. F.
Published: February 2021

Tracer diffusion coefficients obtained from the Taylor dispersion technique at 25.0 °C were measured to study the influence of sodium, ammonium and magnesium salts at 0.01 and 0.1 mol dm−3 on the transport behavior of sodium hyaluronate (NaHy, 0.1%). The selection of these salts was based on their position in Hofmeister series, which describe the specific influence of different ions (cations and anions) on some physicochemical properties of a system that can be interpreted as a salting-in or salting-out effect. In our case, in general, an increase in the ionic strength (i.e., concentrations at 0.01 mol dm−3) led to a significant decrease in the limiting diffusion coefficient of the NaHy 0.1%, indicating, in those circumstances, the presence of salting-in effects. However, the opposite effect (salting-out) was verified with the increase in concentration of some salts, mainly for NH4SCN at 0.1 mol dm−3. In this particular salt, the cation is weakly hydrated and, consequently, its presence does not favor interactions between NaHy and water molecules, promoting, in those circumstances, less resistance to the movement of NaHy and thus to the increase of its diffusion (19%). These data, complemented by viscosity measurements, permit us to have a better understanding about the effect of these salts on the transport behaviour of NaHy.
Lifelong Ulk1-Mediated Autophagy Deficiency in Muscle Induces Mitochondrial Dysfunction and Contractile Weakness
Authors: Nichenko Anna S. , Sorensen Jacob R. , Southern W. Michael , Qualls Anita E. , Schifino Albino G. , McFaline-Figueroa Jennifer , Blum Jamie E. , Tehrani Kayvan F. , Yin Hang , Mortensen Luke J. , Thalacker-Mercer Anna E. , Greising Sarah M. , Call Jarrod A.
Published: February 2021

The accumulation of damaged mitochondria due to insufficient autophagy has been implicated in the pathophysiology of skeletal muscle aging. Ulk1 is an autophagy-related kinase that initiates autophagosome assembly and may also play a role in autophagosome degradation (i.e., autophagy flux), but the contribution of Ulk1 to healthy muscle aging is unclear. Therefore, the purpose of this study was to investigate the role of Ulk1-mediated autophagy in skeletal muscle aging. At age 22 months (80% survival rate), muscle contractile and metabolic function were assessed using electrophysiology in muscle-specific Ulk1 knockout mice (MKO) and their littermate controls (LM). Specific peak-isometric torque of the ankle dorsiflexors (normalized by tibialis anterior muscle cross-sectional area) and specific force of the fast-twitch extensor digitorum longus muscles was reduced in MKO mice compared to LM mice (p < 0.03). Permeabilized muscle fibers from MKO mice had greater mitochondrial content, yet lower mitochondrial oxygen consumption and greater reactive oxygen species production compared to fibers from LM mice (p ≤ 0.04). Alterations in neuromuscular junction innervation patterns as well as changes to autophagosome assembly and flux were explored as possible contributors to the pathological features in Ulk1 deficiency. Of primary interest, we found that Ulk1 phosphorylation (activation) to total Ulk1 protein content was reduced in older muscles compared to young muscles from both human and mouse, which may contribute to decreased autophagy flux and an accumulation of dysfunctional mitochondria. Results from this study support the role of Ulk1-mediated autophagy in aging skeletal muscle, reflecting Ulk1′s dual role in maintaining mitochondrial integrity through autophagosome assembly and degradation.
Leucokinin and Associated Neuropeptides Regulate Multiple Aspects of Physiology and Behavior in Drosophila
Authors: Nässel Dick R.
Published: February 2021

Leucokinins (LKs) constitute a family of neuropeptides identified in numerous insects and many other invertebrates. LKs act on G-protein-coupled receptors that display only distant relations to other known receptors. In adult Drosophila, 26 neurons/neurosecretory cells of three main types express LK. The four brain interneurons are of two types, and these are implicated in several important functions in the fly’s behavior and physiology, including feeding, sleep–metabolism interactions, state-dependent memory formation, as well as modulation of gustatory sensitivity and nociception. The 22 neurosecretory cells (abdominal LK neurons, ABLKs) of the abdominal neuromeres co-express LK and a diuretic hormone (DH44), and together, these regulate water and ion homeostasis and associated stress as well as food intake. In Drosophila larvae, LK neurons modulate locomotion, escape responses and aspects of ecdysis behavior. A set of lateral neurosecretory cells, ALKs (anterior LK neurons), in the brain express LK in larvae, but inconsistently so in adults. These ALKs co-express three other neuropeptides and regulate water and ion homeostasis, feeding, and drinking, but the specific role of LK is not yet known. This review summarizes Drosophila data on embryonic lineages of LK neurons, functional roles of individual LK neuron types, interactions with other peptidergic systems, and orchestrating functions of LK.
Intestinal Goblet Cell Loss during Chorioamnionitis in Fetal Lambs: Mechanistic Insights and Postnatal Implications
Authors: van Gorp Charlotte , de Lange Ilse H. , Massy Kimberly R. I. , Kessels Lilian , Jobe Alan H. , Cleutjens Jack P. M. , Kemp Matthew W. , Saito Masatoshi , Usada Haruo , Newnham John , Hütten Matthias , Kramer Boris W. , Zimmermann Luc J. , Wolfs Tim G. A. M.
Published: February 2021

Chorioamnionitis, an important cause of preterm birth, is linked to necrotizing enterocolitis (NEC). NEC is characterized by a disrupted mucus barrier, goblet cell loss, and endoplasmic reticulum (ER) stress of the intestinal epithelium. These findings prompted us to investigate the mechanisms underlying goblet cell alterations over time in an ovine chorioamnionitis model. Fetal lambs were intra-amniotically (IA) exposed to lipopolysaccharides (LPS) for 5, 12, or 24 h, or 2, 4, 8, or 15 d before premature delivery at 125 d gestational age (GA). Gut inflammation, the number, distribution, and differentiation of goblet cells, ER stress, and apoptosis were measured. We found a biphasic reduction in goblet cell numbers 24 h–2 d after, and 15 d after IA LPS exposure. The second decrease of goblet cell numbers was preceded by intestinal inflammation, apoptosis, and crypt ER stress, and increased SAM-pointed domain-containing ETS transcription factor (SPDEF)-positive cell counts. Our combined findings indicated that ER stress drives apoptosis of maturating goblet cells during chorioamnionitis, ultimately reducing goblet cell numbers. As similar changes have been described in patients suffering from NEC, these findings are considered to be clinically important for understanding the predecessors of NEC, and targeting ER stress in this context is interesting for future therapeutics.
Computational Modulation of the V3 Region of Glycoprotein gp125 of HIV-2
Authors: Serra Patrícia A. , Taveira Nuno , Guedes Rita C.
Published: February 2021

HIV-2 infection is frequently neglected in HIV/AIDS campaigns. However, a special emphasis must be given to HIV-2 as an untreated infection that also leads to AIDS and death, and for which the efficacy of most available drugs is limited against HIV-2. HIV envelope glycoproteins mediate binding to the receptor CD4 and co-receptors at the surface of the target cell, enabling fusion with the cell membrane and viral entry. Here, we developed and optimized a computer-assisted drug design approach of an important HIV-2 glycoprotein that allows us to explore and gain further insights at the molecular level into protein structures and interactions crucial for the inhibition of HIV-2 cell entry. The 3D structure of a key HIV-2ROD gp125 region was generated by a homology modeling campaign. To disclose the importance of the main structural features and compare them with experimental results, 3D-models of six mutants were also generated. These mutations revealed the selective impact on the behavior of the protein. Furthermore, molecular dynamics simulations were performed to optimize the models, and the dynamic behavior was tackled to account for structure flexibility and interactions network formation. Structurally, the mutations studied lead to a loss of aromatic features, which is very important for the establishment of π-π interactions and could induce a structural preference by a specific coreceptor. These new insights into the structure-function relationship of HIV-2 gp125 V3 and surrounding regions will help in the design of better models and the design of new small molecules capable to inhibit the attachment and binding of HIV with host cells.
Deletion of the Spata3 Gene Induces Sperm Alterations and In Vitro Hypofertility in Mice
Authors: Girault Marie-Sophie , Dupuis Sophie , Ialy-Radio Côme , Stouvenel Laurence , Viollet Cécile , Pierre Rémi , Favier Maryline , Ziyyat Ahmed , Barbaux Sandrine
Published: February 2021

Thanks to the analysis of an Interspecific Recombinant Congenic Strain (IRCS), we previously defined the Mafq1 quantitative trait locus as an interval on mouse Chromosome 1 associated with male hypofertility and ultrastructural abnormalities. We identified the Spermatogenesis associated protein 3 gene (Spata3 or Tsarg1) as a pertinent candidate within the Mafq1 locus and performed the CRISPR-Cas9 mediated complete deletion of the gene to investigate its function. Male mice deleted for Spata3 were normally fertile in vivo but exhibited a drastic reduction of efficiency in in vitro fertilization assays. Mobility parameters were normal but ultrastructural analyses revealed acrosome defects and an overabundance of lipids droplets in cytoplasmic remnants. The deletion of the Spata3 gene reproduces therefore partially the phenotype of the hypofertile IRCS strain.
Active Learning with Bayesian UNet for Efficient Semantic Image Segmentation
Authors: Saidu Isah Charles , Csató Lehel
Published: February 2021

We present a sample-efficient image segmentation method using active learning, we call it Active Bayesian UNet, or AB-UNet. This is a convolutional neural network using batch normalization and max-pool dropout. The Bayesian setup is achieved by exploiting the probabilistic extension of the dropout mechanism, leading to the possibility to use the uncertainty inherently present in the system. We set up our experiments on various medical image datasets and highlight that with a smaller annotation effort our AB-UNet leads to stable training and better generalization. Added to this, we can efficiently choose from an unlabelled dataset.
Sustainability in the European Union: Analyzing the Discourse of the European Green Deal
Authors: Eckert Eva , Kovalevska Oleksandra
Published: February 2021

In the European Union, the concern for sustainability has been legitimized by its politically and ecologically motivated discourse disseminated through recent policies of the European Commission and the local as well as international media. In the article, we question the very meaning of sustainability and examine the European Green Deal, the major political document issued by the EC in 2019. The main question pursued in the study is whether expectations verbalized in the Green Deal’s plans, programs, strategies, and developments hold up to the scrutiny of critical discourse analysis. We compare the Green Deal’s treatment of sustainability to how sustainability is presented in environmental and social science scholarship and point out that research, on the one hand, and the politically motivated discourse, on the other, do not correlate and often actually contradict each other. We conclude that sustainability discourse and its keywords, lexicon, and phraseology have become a channel through which political institutions in the EU such as the European Commission sideline crucial environmental issues and endorse their own presence. The Green Deal discourse shapes political and institutional power of the Commission and the EU.
L2 Knowledge of the Obligatory French Subjunctive: Offline Measures and Eye Tracking Compared
Authors: Dudley Amber , Slabakova Roumyana
Published: February 2021

Extensive research has shown that second language (L2) learners find it difficult to apply grammatical knowledge during real-time processing, especially when differences exist between the first (L1) and L2. The current study examines the extent to which British English-speaking learners of French can apply their grammatical knowledge of the French subjunctive during real-time processing, and whether this ability is modulated by the properties of the L1 grammar, and/or proficiency. Data from an acceptability judgment task and an eye-tracking during reading experiment revealed that L2 learners had knowledge of the subjunctive, but were unable to apply this knowledge when reading for comprehension. Such findings therefore suggest that L2 knowledge of the subjunctive, at least at the proficiency levels tested in this study, is largely metalinguistic (explicit) in nature and that reduced lexical access and/or limited computational resources (e.g., working memory) prevented learners from fully utilising their grammatical representations during real-time processing.
Behind the Wall of Indifference: Prisoner Voices about the Realities of Prison Health Care
Authors: Zaitzow Barbara H. , Willis Anthony K.
Published: February 2021

While most Americans never see or become ensnared in the nation’s vast correctional system, there are unprecedented costs—economic, social, and ethical—that are being paid, one way or another, by everyone in this country. It is no secret that prison inmates face health threats behind bars that equal anything they face in the streets. Violent assault, rape, or the outbreak of highly infectious diseases are much more common in correctional facilities than in the general population. Prison conditions can easily fan the spread of disease through overcrowding, poor ventilation, and late or inadequate medical care. Effectively protected from public scrutiny, the prison health care system has almost zero accountability, thus escaping outside attention to serious failures of care. If you want to know about the practice of health care in prison settings, ask someone who has been “in” the system. Prisoners have a story to tell and this article gives voice to the experiences of those who have been directly impacted by the provision of health care in the prison system.
Design Optimization of Reconfigurable Liquid Crystal Patch Antenna
Authors: Kim Dowon , Kim Kitae , Kim Hogyeong , Choi Moonyoung , Na Jun-Hee
Published: February 2021

In various fields such as the 5G antenna system and satellite communication system, there is a growing demand to develop a smart antenna with a frequency selective or beamforming function within a limited space. While antennas utilizing mechanical, electronic, and material characteristics are being studied, a method of having tunable frequency characteristics by applying a liquid crystal material with dielectric anisotropy to a planar patch antenna is proposed. In resonance mode, the design method for using only the minimum amount of expensive liquid crystals is systematically arranged while maximizing the amount of change in the operating frequency of the antenna by considering the electric field distribution on the surface of the patch antenna. Furthermore, to increase the dielectric anisotropy of the liquid crystal, the liquid crystal must be aligned. Simultaneously, in cases where the cell gap of the liquid crystal exceeds 100 μm, the alignment force is weakened. While compensating for this shortcoming, securing the radiation characteristics of the antenna is proposed, and simulations are performed.
Performance of Mortars with Commercially-Available Reactive Magnesium Oxide as Alternative Binder
Authors: Bravo Miguel , Forero Javier A. , Nobre José , de Brito Jorge , Evangelista Luís
Published: February 2021

This paper intends to analyze the performance of mortars with reactive MgO, as a sustainable alternative to cement. Six different MgOs from Australia, Canada, and Spain were used in the production of mortars as partial substitutes for cement, namely 5%, 10%, 15%, 20%, and 25% (by weight). MgOs with different levels of reactivity were used to analyze its influence on the performance of MgO mortars. In order to evaluate the mechanical performance of these mortars, compressive strength, flexural strength, dynamic modulus of elasticity, and ultrasonic pulse velocity tests were performed. Compressive strength tests showed that the use of 25% reactive MgO can cause a decrease of this property of between 28% and 49%. The use of reactive MgO affected the other mechanical properties less. This paper also intends to analyze the durability performance of mortars with reactive MgO. To that effect, water absorption by capillarity was assessed. In this research, the effect of using MgO on the shrinkage was also analyzed. It was found that shrinkage may decrease by more than a half in some cases.
In-Vivo Degradation Behavior and Osseointegration of 3D Powder-Printed Calcium Magnesium Phosphate Cement Scaffolds
Authors: Kowalewicz Katharina , Vorndran Elke , Feichtner Franziska , Waselau Anja-Christina , Brueckner Manuel , Meyer-Lindenberg Andrea
Published: February 2021

Calcium magnesium phosphate cements (CMPCs) are promising bone substitutes and experience great interest in research. Therefore, in-vivo degradation behavior, osseointegration and biocompatibility of three-dimensional (3D) powder-printed CMPC scaffolds were investigated in the present study. The materials Mg225 (Ca0.75Mg2.25(PO4)2) and Mg225d (Mg225 treated with diammonium hydrogen phosphate (DAHP)) were implanted as cylindrical scaffolds (h = 5 mm, Ø = 3.8 mm) in both lateral femoral condyles in rabbits and compared with tricalcium phosphate (TCP). Treatment with DAHP results in the precipitation of struvite, thus reducing pore size and overall porosity and increasing pressure stability. Over 6 weeks, the scaffolds were evaluated clinically, radiologically, with Micro-Computed Tomography (µCT) and histological examinations. All scaffolds showed excellent biocompatibility. X-ray and in-vivo µCT examinations showed a volume decrease and increasing osseointegration over time. Structure loss and volume decrease were most evident in Mg225. Histologically, all scaffolds degraded centripetally and were completely traversed by new bone, in which the remaining scaffold material was embedded. While after 6 weeks, Mg225d and TCP were still visible as a network, only individual particles of Mg225 were present. Based on these results, Mg225 and Mg225d appear to be promising bone substitutes for various loading situations that should be investigated further.
Advanced Technologies for Stabilization and High Performance of Seawater RO Membrane Desalination Plants
Authors: Takabatake Hiroo , Taniguchi Masahide , Kurihara Masaru
Published: February 2021

Seawater desalination plants that use reverse osmosis (RO) membranes have become a core part of social infrastructure, and should be designed to meet the needs of product water quality and production capacity, while considering various environmental factors such as the seawater quality, temperature and geographical features. Furthermore, stable operation while overcoming various problems should be achieved alongside the increasing demands for energy saving and cost reduction. As no universal plant apparatus and operation technology meets these various requirements, the plants need to be customized for individual solutions. This paper reviews and summarizes the proven technologies, including their advantages/disadvantages, and points to cutting-edge technologies related to the design and operation maintenance of seawater intake, pre-treatment and the RO desalination process.
Transporters at the Interface between Cytosolic and Mitochondrial Amino Acid Metabolism
Authors: Hewton Keeley G. , Johal Amritpal S. , Parker Seth J.
Published: February 2021

Mitochondria are central organelles that coordinate a vast array of metabolic and biologic functions important for cellular health. Amino acids are intricately linked to the bioenergetic, biosynthetic, and homeostatic function of the mitochondrion and require specific transporters to facilitate their import, export, and exchange across the inner mitochondrial membrane. Here we review key cellular metabolic outputs of eukaryotic mitochondrial amino acid metabolism and discuss both known and unknown transporters involved. Furthermore, we discuss how utilization of compartmentalized amino acid metabolism functions in disease and physiological contexts. We examine how improved methods to study mitochondrial metabolism, define organelle metabolite composition, and visualize cellular gradients allow for a more comprehensive understanding of how transporters facilitate compartmentalized metabolism.
Formulation of Novel Liquid Crystal (LC) Formulations with Skin-Permeation-Enhancing Abilities of Plantago lanceolata (PL) Extract and Their Assessment on HaCaT Cells
Authors: Kósa Dóra , Pető Ágota , Fenyvesi Ferenc , Váradi Judit , Vecsernyés Miklós , Gonda Sándor , Vasas Gábor , Fehér Pálma , Bácskay Ildikó , Ujhelyi Zoltán
Published: February 2021

Exposure to reactive oxygen species can easily result in serious diseases, such as hyperproliferative skin disorders or skin cancer. Herbal extracts are widely used as antioxidant sources in different compositions. The importance of antioxidant therapy in inflammatory conditions has increased. Innovative formulations can be used to improve the effects of these phytopharmacons. The bioactive compounds of Plantago lanceolata (PL) possess different effects, such as anti-inflammatory, antioxidant, and bactericidal pharmacological effects. The objective of this study was to formulate novel liquid crystal (LC) compositions to protect Plantago lanceolata extract from hydrolysis and to improve its effect. Since safety is an important aspect of pharmaceutical formulations, the biological properties of applied excipients and blends were evaluated using assorted in vitro methods on HaCaT cells. According to the antecedent toxicity screening evaluation, three surfactants were selected (Gelucire 44/14, Labrasol, and Lauroglycol 90) for the formulation. The dissolution rate of PL from the PL-LC systems was evaluated using a Franz diffusion chamber apparatus. The antioxidant properties of the PL-LC systems were evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and malondialdehyde (MDA) assessments. Our results suggest that these compositions use a nontraditional, rapid-permeation pathway for the delivery of drugs, as the applied penetration enhancers reversibly alter the barrier properties of the outer stratum corneum. These excipients can be safe and highly tolerable thus, they could improve the patient’s experience and promote adherence.
Biguanide-Based Synthesis of 1,3,5-Triazine Derivatives with Anticancer Activity and 1,3,5-Triazine Incorporated Calcium Citrate Nanoparticles
Authors: Chalermnon Monnaya , Cherdchom Sarocha , Sereemaspun Amornpun , Rojanathanes Rojrit , Khotavivattana Tanatorn
Published: February 2021

Twelve derivatives of biguanide-derived 1,3,5-triazines, a promising class of anticancer agent, were synthesised and evaluated for their anticancer activity against two colorectal cancer cell lines—HCT116 and SW620. 2c and 3c which are the derivatives containing o-hydroxyphenyl substituents exhibited the highest activity with IC50 against both cell lines in the range of 20–27 µM, which is comparable to the IC50 of cisplatin reference. Moreover, the potential use of the calcium citrate nanoparticles (CaCit NPs) as a platform for drug delivery system was studied on a selected 1,3,5-triazine derivative 2a. Condition optimisation revealed that the source of citrate ions and reaction time significantly influence the morphology, size and %drug loading of the particles. With the optimised conditions, “CaCit-2a NPs” were successfully synthesised with the size of 148 ± 23 nm and %drug loading of up to 16.3%. Furthermore, it was found that the release of 2a from the synthesised CaCit-2a NPs is pH-responsive, and 2a could be control released under the acidic cancer environment. The knowledge from this study is perceptive for further development of the 1,3,5-triazine-based anticancer drugs and provide the platform for the incorporation of other drugs in the CaCit NPs in the future.
Medicinal Use of Testosterone and Related Steroids Revisited
Authors: Tauchen Jan , Jurášek Michal , Huml Lukáš , Rimpelová Silvie
Published: February 2021

Testosterone derivatives and related compounds (such as anabolic-androgenic steroids—AAS) are frequently misused by athletes (both professional and amateur) wishing to promote muscle development and strength or to cover AAS misuse. Even though these agents are vastly regarded as abusive material, they have important pharmacological activities that cannot be easily replaced by other drugs and have therapeutic potential in a range of conditions (e.g., wasting syndromes, severe burns, muscle and bone injuries, anemia, hereditary angioedema). Testosterone and related steroids have been in some countries treated as controlled substances, which may affect the availability of these agents for patients who need them for therapeutic reasons in a given country. Although these agents are currently regarded as rather older generation drugs and their use may lead to serious side-effects, they still have medicinal value as androgenic, anabolic, and even anti-androgenic agents. This review summarizes and revisits the medicinal use of compounds based on the structure and biological activity of testosterone, with examples of specific compounds. Additionally, some of the newer androgenic-anabolic compounds are discussed such as selective androgen receptor modulators, the efficacy/adverse-effect profiles of which have not been sufficiently established and which may pose a greater risk than conventional androgenic-anabolic agents.
Overview of the Assays to Probe O-Linked β-N-Acetylglucosamine Transferase Binding and Activity
Authors: Balsollier Cyril , Pieters Roland J. , Anderluh Marko
Published: February 2021

O-GlcNAcylation is a posttranslational modification that occurs at serine and threonine residues of protein substrates by the addition of O-linked β-d-N-acetylglucosamine (GlcNAc) moiety. Two enzymes are involved in this modification: O-GlcNac transferase (OGT), which attaches the GlcNAc residue to the protein substrate, and O-GlcNAcase (OGA), which removes it. This biological balance is important for many biological processes, such as protein expression, cell apoptosis, and regulation of enzyme activity. The extent of this modification has sparked interest in the medical community to explore OGA and OGT as therapeutic targets, particularly in degenerative diseases. While some OGA inhibitors are already in phase 1 clinical trials for the treatment of Alzheimer’s disease, OGT inhibitors still have a long way to go. Due to complex expression and instability, the discovery of potent OGT inhibitors is challenging. Over the years, the field has grappled with this problem, and scientists have developed a number of techniques and assays. In this review, we aim to highlight assays and techniques for OGT inhibitor discovery, evaluate their strength for the field, and give us direction for future bioassay methods.
Rutin-Loaded Solid Lipid Nanoparticles: Characterization and In Vitro Evaluation
Authors: De Gaetano Federica , Cristiano Maria Chiara , Venuti Valentina , Crupi Vincenza , Majolino Domenico , Paladini Giuseppe , Acri Giuseppe , Testagrossa Barbara , Irrera Alessia , Paolino Donatella , Tommasini Silvana , Ventura Cinzia Anna , Stancanelli Rosanna
Published: February 2021

This study was aimed at preparing and characterizing solid lipid nanoparticles loading rutin (RT-SLNs) for the treatment of oxidative stress-induced diseases. Phospholipon 80H® as a solid lipid and Polysorbate 80 as surfactant were used for the SLNs preparation, using the solvent emulsification/diffusion method. We obtained spherical RT-SLNs with low sizes, ranging from 40 to 60 nm (hydrodynamic radius) for the SLNs prepared starting from 2% and 5% (w/w) theoretical amount. All prepared formulations showed negative zeta-potential values. RT was efficiently encapsulated within SLNs, obtaining high encapsulation efficiency and drug content percentages, particularly for SLNs prepared with a 5% theoretical amount of RT. In vitro release profiles and analysis of the obtained data applying different kinetic models revealed Fickian diffusion as the main mechanism of RT release from the SLNs. The morphology of RT-SLNs was characterized by scanning electron microscopy (SEM), whereas the interactions between RT and the lipid matrix were investigated by Raman spectroscopy, evidencing spectral modifications of characteristic bands of RT due to the establishment of new interactions. Finally, antioxidant activity assay on human glioblastoma astrocytoma (U373) culture cells showed a dose-dependent activity for RT-SLNs, particularly at the highest assayed dose (50 μM), whereas the free drug showed the lesser activity.