bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis and Preliminary Evaluation of an ASGPr-Targeted Polycationic β-Cyclodextrin Carrier for Nucleosides and Nucleotides
Authors: Ryu Jang-Ha , Zheng Weizhong , Yang Xiao-Hong , Elsaidi Hassan , Diakur Jim , Wiebe Leonard I.
Published: February 2024

Most antiviral and anticancer nucleosides are prodrugs that require stepwise phosphorylation to their triphosphate nucleotide form for biological activity. Monophosphorylation may be rate-limiting, and the nucleotides may be unstable and poorly internalized by target cells. Effective targeting and delivery systems for nucleoside drugs, including oligonucleotides used in molecular therapeutics, could augment their efficacy. The development of a carrier designed to effect selective transmembrane internalization of nucleotides via the asialoglycoprotein receptor (ASGPr) is now reported. In this work, the polycationic, polygalactosyl drug delivery carrier heptakis[6-amino-6-deoxy-2-O-(3-(1-thio-β-D-galactopyranosyl)-propyl)]-β-cyclodextrin hepta-acetate salt (GCyDAc), potentially a bifunctional carrier of (poly)nucleotides, was modeled by molecular docking in silico as an ASGPr-ligand, then synthesized for testing. The antivirals arabinosyl adenine (araA, vidarabine, an early generation antiviral nucleoside), arabinosyl adenine 5′-monophosphate (araAMP), and 12-mer-araAMP (p-araAMP) were selected for individual formulation with GCyDAc to develop this concept. Experimentally, beta cyclodextrin was decorated with seven protonated amino substituents on the primary face, and seven thiogalactose residues on its secondary face. AraA, araAMP, and p-araAMP were individually complexed with GCyDAc and complex formation for each drug was confirmed by differential scanning calorimetry (DSC). Finally, the free drugs and their GCyDAc complexes were evaluated for antiviral activity using ASGPr-expressing HepAD38 cells in cell culture. In this model, araA, araAMP, and p-araAMP showed relative antiviral potencies of 1.0, 1.1, and 1.2, respectively. In comparison, GCyDAc-complexes of araA, araAMP, and p-araAMP were 2.5, 1.3, and 1.2 times more effective than non-complexed araA in suppressing viral DNA production. The antiviral potencies of these complexes were minimally supportive of the hypothesis that ASGPr-targeted, CyD-based charge-association complexation of nucleosides and nucleotides could effectively enhance antiviral efficacy. GCyDAc was non-toxic to mammalian cells in cell culture, as determined using the MTS proliferation assay.
Design Optimization of Silicon-Based Optically Excited Terahertz Wave Modulation
Authors: Zhao Chenyu , Wang Dayong , Lin Shufeng , Zhao Jie , Wang Yunxin , Rong Lu
Published: February 2024

The modulation of a terahertz (THz) wave on amplitude, phase and polarization is important for the application of THz technology, especially in the field of imaging, and is one of the current research hotspots. Silicon-based, optically excited THz modulator is a wavefront modulation technique with a simple, compact and reconfigurable optical path. It can realize the dynamic modulation of THz wavefronts by only changing the projected two-dimensional pattern, but it still suffers from the problems of lower modulation efficiency and slower modulation rates. In this article, the Drude model in combination with the multiple thin layers structure model and Fresnel matrix method is used to compare the modulation efficiencies of three modulation modes and more factors. The method is more accurate than the popular proposed method, especially when the thickness of the excited photoconductive layers reaches a few hundred microns. In comparing the three modes, namely transmission, ordinary reflection and total internal reflection, it is found the total internal reflection modulation mode has the best modulation efficiency. Further, under this mode, the effects of three factors, including the lifetime of photo-excited carriers, the wavelength of pump light and the frequency of THz wave, on the performance of THz modulator are analyzed. The simulation results show that the realization of total internal reflection using silicon prisms is a simple and effective method to improve the modulation efficiency of a silicon-based optically excited THz modulator, which provides references for the design of a photo-induced THz modulator.
Laser Remote Sensing of Seismic Wave with Sub-Millimeter Scale Amplitude Based on Doppler Characteristics Extracted from Wavefront Sensor
Authors: Luo Quan , Luo Hongsheng , Wu Guihan , Ji Xiang , Su Jinshan , Jiang Wei
Published: February 2024

Laser remote sensing of earthquake waves has the potential to be used in many applications. This article shows a Doppler model for laser remote sensing of seismic waves based on a wavefront sensor. The longitudinal vibration wave is analyzed using remote sensing, guided by theoretical principles. To determine the magnitude of ground vibration, we employ the method of wavefront phase change analysis, utilizing a continuous laser emitting light with a wavelength of 635 nm to illuminate the ground target. The ground vibration amplitude within the range of 0.12–1.18 mm was examined, confirming the reasonableness of the Doppler model. Simultaneously, the experimental findings indicate that the system exhibits a certain enhancement in detection accuracy compared to the conventional laser remote sensing detection technique. This approach can detect vibration signals at a sub-millimeter scale level, with an accuracy of 1% to 2%. The approach can fulfill the requirements for detecting seismic waves with low frequencies.
Quantum Dynamics of Cavity–Bose–Einstein Condensates in a Gravitational Field
Authors: Li Zhen , Lu Wang-Jun , Jiao Ya-Feng
Published: February 2024

We theoretically studied the quantum dynamics of a cavity–Bose–Einstein condensate (BEC) system in a gravitational field, which is composed of a Fabry–Pérot cavity and a BEC. We also show how to deterministically generate the transient macroscopic quantum superposition states (MQSSs) of the cavity by the use of optomechanical coupling between the cavity field and the BEC. The quantum dynamics of the cavity–BEC system specifically include phase space trajectory dynamics, system excitation number dynamics, quantum entanglement dynamics, and quantum coherence dynamics. We found that the system performs increasingly complex trajectories for larger values of the Newtonian gravity parameter. Moreover, the number of phonon excitations of the system can be increased by coupling the cavity–BEC system to Newtonian gravity, which is analogous to an external direct current drive. The scattering of atoms inside the BEC affects the periodicity of the quantum dynamics of the system. We demonstrate a curious complementarity relation between the quantum entanglement and quantum coherence of cavity–BEC systems and found that the complementarity property can be sustained to some extent, despite being in the presence of the cavity decay. This phenomenon also goes some way to show that quantum entanglement and quantum coherence can be referred to together as quantum resources.
Study on the Generation of 1.9 μm Mode Superposition Conversion Laser by Double-End Off-Axis Pumping
Authors: Li Chao , Chen Xinyu , Sun Ye , Liu Jingliang , Jin Guangyong
Published: February 2024

In this paper, the Laguerre–Gaussian (LG) mode superposition is obtained by using the technology of double-end off-axis pumping Tm:YLF crystal, and the LG mode superposition is achieved by combining the extra-cavity conversion method. The impact of changing the off-axis distance on the order of Hermite–Gaussian (HG) mode and the topological charge of LG mode is studied. The results show that when the off-axis distance of the pump source at both ends is tuned, when the off-axis distance is in the range of 260 μm~845 μm, the single-ended 0~10 order HG mode can be obtained. Subsequently, the mode converter is placed to obtain the LG mode beam, and the double-end simultaneously pumps the crystal to obtain the superimposed LG mode. The tuning off-axis quantity changes the topological charge number. When P = 0, l1=l2, the superimposed LG mode is a single-ring spot, and the vortex beam center’s dark hollow area increases with the topological charge number. When P = 0, l1=−l2, the superimposed LG mode is a petal-like spot. The number of petals differs from the topological charges of two opposite numbers. Finally, in the case of changing the topological charge number of the double-ended LG mode, the output of the vortex array structured beams of the tuning mode order 1.9 μm Tm:YLF is completed in the case of conversion and superposition.
Design Method for Freeform Off-Axis Three-Mirror Anastigmat Optical Systems with a Large Field of View and Low Error Sensitivity
Authors: Ren Chengming , Meng Qingyu
Published: February 2024

A freeform off-axis three-mirror anastigmat (TMA) optical system with a large field of view (FOV) can obtain target image information with a larger spatial range and more spatial details, which is a development trend within the realm of space optics. The optical aberration increases exponentially with the FOV, resulting in a significant increase in error sensitivity for large-FOV optical systems. To address this issue, a method for designing optical systems with a large FOV and low error sensitivity is proposed. The FOV is gradually expanded from a small initial value in equal-length increments until it reaches the full FOV. At each step, the error sensitivity is recalculated and controlled to a lesser extent than in the previous step. In this design process, the freeform surface is used to correct the aberration and obtain low error sensitivity. An optical system with a focal length of 1000 mm and an F-number of 10 is used as an example, and the FOV is enlarged from 5° × 1° to 20° × 4°. The design results show that the modulation transfer function (MTF) of the optical system can reach 0.45@50 lp/mm, and the average wavefront aberration is 0.029λ. After four rounds of FOV expansion and error sensitivity optimization, the error sensitivity is reduced by 37.27% compared to the initial system, which verifies the correctness and practicality of the method.
ZmADF5, a Maize Actin-Depolymerizing Factor Conferring Enhanced Drought Tolerance in Maize
Authors: Liu Bojuan , Wang Nan , Yang Ruisi , Wang Xiaonan , Luo Ping , Chen Yong , Wang Fei , Li Mingshun , Weng Jianfeng , Zhang Degui , Yong Hongjun , Han Jienan , Zhou Zhiqiang , Zhang Xuecai , Hao Zhuanfang , Li Xinhai
Published: February 2024

Drought stress is seriously affecting the growth and production of crops, especially when agricultural irrigation still remains quantitatively restricted in some arid and semi-arid areas. The identification of drought-tolerant genes is important for improving the adaptability of maize under stress. Here, we found that a new member of the actin-depolymerizing factor (ADF) family; the ZmADF5 gene was tightly linked with a consensus drought-tolerant quantitative trait locus, and the significantly associated signals were detected through genome wide association analysis. ZmADF5 expression could be induced by osmotic stress and the application of exogenous abscisic acid. Its overexpression in Arabidopsis and maize helped plants to keep a higher survival rate after water-deficit stress, which reduced the stomatal aperture and the water-loss rate, as well as improved clearance of reactive oxygen species. Moreover, seventeen differentially expressed genes were identified as regulated by both drought stress and ZmADF5, four of which were involved in the ABA-dependent drought stress response. ZmADF5-overexpressing plants were also identified as sensitive to ABA during the seed germination and seedling stages. These results suggested that ZmADF5 played an important role in the response to drought stress.
New Alien Plant Taxa for Italy and Europe: An Update
Authors: Musarella Carmelo Maria , Laface Valentina Lucia Astrid , Angiolini Claudia , Bacchetta Gianluigi , Bajona Enrico , Banfi Enrico , Barone Giulio , Biscotti Nello , Bonsanto Daniele , Calvia Giacomo , Cambria Salvatore , Capuano Alberto , Caruso Giuseppe , Crisafulli Alessandro , Del Guacchio Emanuele , Di Gristina Emilio , Domina Gianniantonio , Fanfarillo Emanuele , Fascetti Simonetta , Fiaschi Tiberio , Galasso Gabriele , Mascia Francesco , Mazzacuva Giuliana , Mei Giacomo , Minissale Pietro , Motti Riccardo , Perrino Enrico Vito , Picone Rosa Maria , Pinzani Lorenzo , Podda Lina , Potenza Giovanna , Rosati Leonardo , Stinca Adriano , Tavilla Gianmarco , Villano Clizia , Wagensommer Robert Philipp , Spampinato Giovanni
Published: February 2024

Despite the wide amount of scientific contributions published on alien plant species, their diffusion dynamics, and their interactions with native taxa, it is increasingly difficult to slow down their spreading and their negative impact on habitats. Last recent years, in fact, a sharp rise in the number of new alien plant taxa introduced in Italy and Europe has been recorded. The aim of this work is to investigate most of the Italian territory in order to verify whether this alarming trend is still underway. Specimen collections and/or observations of alien plants have been performed in as many as 12 Italian regions. All the collected specimens are stored in public or private herbaria. Taxa have been identified according to the literature from the countries of origin of the investigated taxa, while the nomenclature followed the current international references. Updates on 106 taxa are reported. In particular, among 117 new records, 89 are first records, 27 are changes to status and there is 1 extinction. Seven new taxa for Italian alien flora are reported, two of which are new to Europe. The administrative regions with the highest number of records are Calabria (48), Sardegna (17) and Sicilia (15). Five of the surveyed taxa, for the first time, have been considered invasive aliens to Italian territory. The unfrequent amount of original results provided by this work, over the simple importance of data itself, proves how floristic investigation, still today, represents one of the most effective tools in broadening the current knowledge about alien taxa and their dynamics.
Sinorhizobium meliloti GR4 Produces Chromosomal- and pSymA-Encoded Type IVc Pili That Influence the Interaction with Alfalfa Plants
Authors: Carvia-Hermoso Cristina , Cuéllar Virginia , Bernabéu-Roda Lydia M. , van Dillewijn Pieter , Soto María J.
Published: February 2024

Type IVc Pili (T4cP), also known as Tad or Flp pili, are long thin microbial filaments that are made up of small-sized pilins. These appendages serve different functions in bacteria, including attachment, biofilm formation, surface sensing, motility, and host colonization. Despite their relevant role in diverse microbial lifestyles, knowledge about T4cP in bacteria that establish symbiosis with legumes, collectively referred to as rhizobia, is still limited. Sinorhizobium meliloti contains two clusters of T4cP-related genes: flp-1 and flp-2, which are located on the chromosome and the pSymA megaplasmid, respectively. Bundle-forming pili associated with flp-1 are involved in the competitive nodulation of alfalfa plants, but the role of flp-2 remains elusive. In this work, we have performed a comprehensive bioinformatic analysis of T4cP genes in the highly competitive S. meliloti GR4 strain and investigated the role of its flp clusters in pilus biogenesis, motility, and in the interaction with alfalfa. Single and double flp-cluster mutants were constructed on the wild-type genetic background as well as in a flagellaless derivative strain. Our data demonstrate that both chromosomal and pSymA flp clusters are functional in pili biogenesis and contribute to surface translocation and nodule formation efficiency in GR4. In this strain, the presence of flp-1 in the absence of flp-2 reduces the competitiveness for nodule occupation.
Modulation of Drought-Induced Stress in Cowpea Genotypes Using Exogenous Salicylic Acid
Authors: Melo Alberto Soares de , Costa Rayssa Ribeiro da , Sá Francisco Vanies da Silva , Dias Guilherme Felix , Alencar Rayanne Silva de , Viana Priscylla Marques de Oliveira , Peixoto Tayd Dayvison Custódio , Suassuna Janivan Fernandes , Brito Marcos Eric Barbosa , Ferraz Rener Luciano de Souza , Costa Patrícia da Silva , Melo Yuri Lima , Corrêa Élida Barbosa , Lacerda Claudivan Feitosa de , Dantas Neto José
Published: February 2024

Plant endogenous mechanisms are not always sufficient enough to mitigate drought stress, therefore, the exogenous application of elicitors, such as salicylic acid, is necessary. In this study, we assessed the mitigating action of salicylic acid (SA) in cowpea genotypes under drought conditions. An experiment was conducted with two cowpea genotypes and six treatments of drought stress and salicylic acid (T1 = Control, T2 = drought stress (stress), T3 = stress + 0.1 mM of SA, T4 = stress + 0.5 mM of SA, T5 = stress + 1.0 mM of SA, and T6 = stress + 2.0 mM of SA). Plants were evaluated in areas of leaf area, stomatal conductance, photosynthesis, proline content, the activity of antioxidant enzymes, and dry grain production. Drought stress reduces the leaf area, stomatal conductance, photosynthesis, and, consequently, the production of both cowpea genotypes. The growth and production of the BRS Paraguaçu genotype outcompetes the Pingo de Ouro-1-2 genotype, regardless of the stress conditions. The exogenous application of 0.5 mM salicylic acid to cowpea leaves increases SOD activity, decreases CAT activity, and improves the production of both genotypes. The application of 0.5 mM of salicylic acid mitigates drought stress in the cowpea genotype, and the BRS Paraguaçu genotype is more tolerant to drought stress.
The MYB-CC Transcription Factor PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) Functions in Phosphate Homeostasis and Affects Salt Stress Tolerance in Rice
Authors: Yang Won Tae , Bae Ki Deuk , Lee Seon-Woo , Jung Ki Hong , Moon Sunok , Basnet Prakash , Choi Ik-Young , Um Taeyoung , Kim Doh Hoon
Published: February 2024

Inorganic phosphate (Pi) homeostasis plays an important role in plant growth and abiotic stress tolerance. Several MYB-CC transcription factors involved in Pi homeostasis have been identified in rice (Oryza sativa). PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) is a class II MYC-CC protein, in which the MYC-CC domain is located at the N terminus. In this study, we established that OsPHL7 is localized to the nucleus and that the encoding gene is induced by Pi deficiency. The Pi-responsive genes and Pi transporter genes are positively regulated by OsPHL7. The overexpression of OsPHL7 enhanced the tolerance of rice plants to Pi starvation, whereas the RNA interference-based knockdown of this gene resulted in increased sensitivity to Pi deficiency. Transgenic rice plants overexpressing OsPHL7 produced more roots than wild-type plants under both Pi-sufficient and Pi-deficient conditions and accumulated more Pi in the shoots and roots. In addition, the overexpression of OsPHL7 enhanced rice tolerance to salt stress. Together, these results demonstrate that OsPHL7 is involved in the maintenance of Pi homeostasis and enhances tolerance to Pi deficiency and salt stress in rice.
Prediction of Historical, Current, and Future Configuration of Tibetan Medicinal Herb Gymnadenia orchidis Based on the Optimized MaxEnt in the Qinghai–Tibet Plateau
Authors: Li Ming , Zhang Yi , Yang Yongsheng , Wang Tongxin , Wu Chu , Zhang Xiujuan
Published: February 2024

Climate change plays a pivotal role in shaping the shifting patterns of plant distribution, and gaining insights into how medicinal plants in the plateau region adapt to climate change will be instrumental in safeguarding the rich biodiversity of the highlands. Gymnosia orchidis Lindl. (G. orchidis) is a valuable Tibetan medicinal resource with significant medicinal, ecological, and economic value. However, the growth of G. orchidis is severely constrained by stringent natural conditions, leading to a drastic decline in its resources. Therefore, it is crucial to study the suitable habitat areas of G. orchidis to facilitate future artificial cultivation and maintain ecological balance. In this study, we investigated the suitable zones of G. orchidis based on 79 occurrence points in the Qinghai–Tibet Plateau (QTP) and 23 major environmental variables, including climate, topography, and soil type. We employed the Maximum Entropy model (MaxEnt) to simulate and predict the spatial distribution and configuration changes in G. orchidis during different time periods, including the last interglacial (LIG), the Last Glacial Maximum (LGM), the Mid-Holocene (MH), the present, and future scenarios (2041–2060 and 2061–2080) under three different climate scenarios (SSP126, SSP370, and SSP585). Our results indicated that annual precipitation (Bio12, 613–2466 mm) and mean temperature of the coldest quarter (Bio11, −5.8–8.5 °C) were the primary factors influencing the suitable habitat of G. orchidis, with a cumulative contribution of 78.5%. The precipitation and temperature during the driest season had the most significant overall impact. Under current climate conditions, the suitable areas of G. orchidis covered approximately 63.72 × 104/km2, encompassing Yunnan, Gansu, Sichuan, and parts of Xizang provinces, with the highest suitability observed in the Hengduan, Yunlin, and Himalayan mountain regions. In the past, the suitable area of G. orchidis experienced significant changes during the Mid-Holocene, including variations in the total area and centroid migration direction. In future scenarios, the suitable habitat of G. orchidis is projected to expand significantly under SSP370 (30.33–46.19%), followed by SSP585 (1.41–22.3%), while contraction is expected under SSP126. Moreover, the centroids of suitable areas exhibited multidirectional movement, with the most extensive displacement observed under SSP585 (100.38 km2). This study provides a theoretical foundation for the conservation of biodiversity and endangered medicinal plants in the QTP.
Synthesis of L-Lactide from Lactic Acid and Production of PLA Pellets: Full-Cycle Laboratory-Scale Technology
Authors: Aliev Gadir , Toms Roman , Melnikov Pavel , Gervald Alexander , Glushchenko Leonid , Sedush Nikita , Chvalun Sergei
Published: February 2024

Lactide is one of the most popular and promising monomers for the synthesis of biocompatible and biodegradable polylactide and its copolymers. The goal of this work was to carry out a full cycle of polylactide production from lactic acid. Process conditions and ratios of reagents were optimized, and the key properties of the synthesized polymers were investigated. The influence of synthesis conditions and the molecular weight of lactic acid oligomers on the yield of lactide was studied. Lactide polymerization was first carried out in a 500 mL flask and then scaled up and carried out in a 2000 mL laboratory reactor setup with a combined extruder. Initially, the lactic acid solution was concentrated to remove free water; then, the oligomerization and synthesis of lactide were carried out in one flask in the presence of various concentrations of tin octoate catalyst at temperatures from 150 to 210 °C. The yield of lactide was 67–69%. The resulting raw lactide was purified by recrystallization in solvents. The yield of lactide after recrystallization in butyl acetate (selected as the optimal solvent for laboratory purification) was 41.4%. Further, the polymerization of lactide was carried out in a reactor unit at a tin octoate catalyst concentration of 500 ppm. Conversion was 95%; Mw = 228 kDa; and PDI = 1.94. The resulting products were studied by differential scanning calorimetry, NMR spectroscopy and gel permeation chromatography. The resulting polylactide in the form of pellets was obtained using an extruder and a pelletizer.
Natural Extracts and Their Applications in Polymer-Based Active Packaging: A Review
Authors: Li Jiawei , Sun Hui , Weng Yunxuan
Published: February 2024

At a time when food safety awareness is increasing, attention is paid not only to food and additives but also to packaging materials. Most current food packaging is usually made of traditional petroleum-based polymeric materials, which are not biodegradable and have adverse effects on the environment and health. In this context, the development of new non-toxic and biodegradable materials for extending the best-before date of food is receiving increasing attention. In addition, additives in packaging materials may migrate outward, resulting in contact with food. For this reason, additives are also seen as a transition from synthetic additives to natural extracts. Active extracts from animals and plants having good antioxidant and antibacterial properties are also beneficial for human health. It is indisputable that active extracts are ideal substitutes for synthetic additives. Polymer packaging materials combined with active extracts not only maintain their original mechanical and optical properties and thermal stability but also endow polymers with new functions to extend the shelf life of food. This review paper provides an overview of this promising natural extract-containing polymer-based active packaging, with a focus on plant essential oils (containing phenolics, monoterpenes, terpene alcohols, terpene ketones, and aldehydes), pigments (procyanidins), vitamins (vitamin B), and peptides (nisin). In particular, this paper covers the research progress of such active extracts, in single or compound forms, combined with diverse polymers (mostly biopolymers) for food packaging applications with particular focus on the antioxidant and antibacterial properties of packaging materials.
Kinetics Study of PVA Polymer by Model-Free and Model-Fitting Methods Using TGA
Authors: Alhulaybi Zaid Abdulhamid , Dubdub Ibrahim
Published: February 2024

Thermogravimetric Analysis (TGA) serves a pivotal technique for evaluating the thermal behavior of Polyvinyl alcohol (PVA), a polymer extensively utilized in the production of fibers, films, and membranes. This paper targets the kinetics of PVA thermal degradation using high three heating rate range 20, 30, and 40 K min−1. The kinetic study was performed using six model-free methods: Freidman (FR), Flynn-Wall-Qzawa (FWO), Kissinger-Akahira-Sunose (KAS), Starink (STK), Kissinger (K), and Vyazovkin (VY) for the determination of the activation energy (Ea). TGA showed two reaction stages: the main one at 550–750 K and the second with 700–810 K. But only the first step has been considered in calculating Ea. The average activation energy values for the conversion range (0.1–0.7) are between minimum 104 kJ mol−1 by VY to maximum 199 kJ mol−1 by FR. Model-fitting has been applied by combing Coats–Redfern (CR) with the master plot (Criado’s) to identify the most convenient reaction mechanism. Ea values gained by the above six models were very similar with the average value of (126 kJ mol−1) by CR. The reaction order models-Second order (F2) was recommended as the best mechanism reaction for PVA pyrolysis. Mechanisms were confirmed by the compensation effect. Finally, (∆H, ∆G, and ∆S) parameters were presented and proved that the reaction is endothermic.
Evaluation of Egg Quality and Performance in Late-Lay Hens Fed Different Combinations of Copper, Manganese, and Zinc Complexed with Sulfate or Amino Acid Ion
Authors: Domel Jill R. , House Gabrielle M. , Sobotik Eric B. , Archer Gregory S.
Published: February 2024

Dietary inclusion of copper (Cu), manganese (Mn), and zinc (Zn) can improve egg shell quality through changing the membrane structure. This study aimed to compare the responses of egg shell to different mineral sources. In this study, 60-week-old laying hens (n = 378) were assigned to one of seven treatments with 18 replicates each in an RCBD. Treatments included the following: control (basal + sulfated minerals (CuSO4, MnSO4, and ZnSO4)), and basal + amino acid complexed (AAC) minerals (AAC Cu, AAC Mn, AAC Zn, AAC Cu + Mn, AAC Mn + Zn, AAC Zn + Cu). Trace minerals were added to a basal diet containing 20 ppm MnSO4 and 20 ppm ZnSO4 to achieve overall target concentrations of 20 ppm Cu, 60 ppm Mn, and 60 ppm Zn. The hens were fed the treatment diet for 15 weeks, and egg production and egg quality were assessed during weeks 5, 10, and 15 of the experiment. Egg shells, egg contents, and excreta were analyzed for Cu, Mn, Zn, Ca, and P during weeks 10 and 15. No treatment differences (p > 0.05) were observed for production or egg quality. Differences between excreta mineral content were observed. The mineral content of egg shells and egg contents did not differ (p > 0.05) at any time point. The mineral source did not affect egg mineral deposition and egg quality measures (p > 0.05). Some AAC trace minerals enhanced retention of zinc, calcium, and manganese, although AAC Cu increased Cu excretion. Taken together, feeding AAC trace minerals does not significantly affect egg production or egg quality during the late-lay period. More research is needed to demonstrate whether Cu excretion is increased when feeding AAC Cu due to increased bioavailability or other factors.
Optimizing Chitin Extraction and Chitosan Production from House Cricket Flour
Authors: Espinosa-Solís Andrea , Velázquez-Segura Angélica , Lara-Rodríguez Carlos , Martínez Luz María , Chuck-Hernández Cristina , Rodríguez-Sifuentes Lucio
Published: February 2024

Chitin and its derivative, chitosan, have diverse applications in fields such as agriculture, medicine, and biosensors, amongst others. Extraction is primarily conducted from marine sources, such as crustaceans, which have been the focus of process optimization studies. However, there are other sources that are more readily available, such as insects, where insufficient research has been conducted. The house cricket (Acheta domesticus) is a promising source for chitin extraction because of its high chitin content, availability, and short lifespan. Modern chemical chitin extraction methods have not been standardized due to the use of different reagents, molar concentrations, temperatures, and reaction times across publications. Therefore, in this study, the composition of Acheta domesticus cricket flour was determined: 2.62% humidity, 4.3% ash content, 56.29% protein, 13.35% fat, 23.44% carbohydrates, and 15.71% crude fiber content. After a drying, defatting, demineralization, deproteinization, and bleaching process, chitin extraction was performed, and chitosan was obtained via a deacetylation reaction. The demineralization process was standardized at 30 °C for 3 h using HCl 2 M, resulting in 95.85 ± 0.012%. The deproteinization process was optimized at 80 °C for 45 min using NaOH 2.56 M, yielding 43.23 ± 1.25%. Finally, the identity and physicochemical characteristics of the compounds were confirmed and determined through characterization with Fourier-Transform Infrared Spectroscopy, X-ray Diffraction, Scanning Electron Microscopy, and Differential Scanning Calorimetry.
Novel Sodium Chloride/Aluminum Oxide Powder-Composite Structure with High Shape-Retention Performance for the Encapsulation of a High-Temperature Phase-Change Material
Authors: Yamashita Seiji , Fuhai Bao , Shenghao Liao , Kita Hideki , Hong Fangjun
Published: February 2024

Inorganic phase-change materials (PCMs) with high melting points have great potential for thermal energy storage systems. Sodium chloride (NaCl) has a high melting point (801 °C) and high latent-heat-storage density (482 kJ/kg). However, it is difficult to encapsulate NaCl using a sintered ceramic shell because of its good wettability against ceramics and high volume-expansion capacity during melting. In this study, a novel NaCl/Al2O3 powder-composite structure was developed as highly stable PCM core material for highly stable encapsulation. The shape-retention performance and the mechanism of NaCl/Al2O3 powder-composite structure during melting were investigated. We have successfully fabricated a NaCl/Al2O3 powder-composite structure, which has a higher NaCl volume ratio of 80 vol% than conventional techniques. The gel-like network structure of Al2O3 particles in molten NaCl was a key structure to keep the shape of the composite ball and to prevent the evaporation of molten NaCl.
Measurement Method of Bar Unmanned Warehouse Area Based on Binocular Vision
Authors: Yan Shuzong , Xu Dong , Yan He , Wang Ziqiang , He Hainan , Wang Xiaochen , Yang Quan
Published: February 2024

With the development of Industry 4.0 and the implementation of the 14th Five-Year Plan, intelligent manufacturing has become a significant trend in the steel industry, which can propel the steel industry toward a more intelligent, efficient, and sustainable direction. At present, the operation mode of unmanned warehouse area for slabs and coils has become relatively mature, while the positioning accuracy requirement of bars is getting more stringent because they are stacked in the warehouse area according to the stacking position and transferred by disk crane. Meanwhile, the traditional laser ranging and line scanning method cannot meet the demand for precise positioning of the whole bundle of bars. To deal with the problems above, this paper applies machine vision technology to the unmanned warehouse area of bars, proposing a binocular vision-based measurement method. On the one hand, a 3D reconstruction model with sub-pixel interpolation is established to improve the accuracy of 3D reconstruction in the warehouse area. On the other hand, a feature point matching algorithm based on motion trend constraint is established by means of multi-sensor data fusion, thus improving the accuracy of feature point matching. Finally, a high-precision unmanned 3D reconstruction of the bar stock area is completed.
Multiperiod Modeling and Optimization of Hydrogen-Based Dense Energy Carrier Supply Chains
Authors: Kakodkar Rahul , Allen R. Cory , Demirhan C. Doga , Fu Xiao , Pappas Iosif , Mutlu Mete , Pistikopoulos Efstratios N.
Published: February 2024

The production of hydrogen-based dense energy carriers (DECs) has been proposed as a combined solution for the storage and dispatch of power generated through intermittent renewables. Frameworks that model and optimize the production, storage, and dispatch of generated energy are important for data-driven decision making in the energy systems space. The proposed multiperiod framework considers the evolution of technology costs under different levels of promotion through research and targeted policies, using the year 2021 as a baseline. Furthermore, carbon credits are included as proposed by the 45Q tax amendment for the capture, sequestration, and utilization of carbon. The implementation of the mixed-integer linear programming (MILP) framework is illustrated through computational case studies to meet set hydrogen demands. The trade-offs between different technology pathways and contributions to system expenditure are elucidated, and promising configurations and technology niches are identified. It is found that while carbon credits can subsidize carbon capture, utilization, and sequestration (CCUS) pathways, substantial reductions in the cost of novel processes are needed to compete with extant technology pathways. Further, research and policy push can reduce the levelized cost of hydrogen (LCOH) by upwards of 2 USD/kg.
Proposal of Industry 5.0-Enabled Sustainability of Product–Service Systems and Its Quantitative Multi-Criteria Decision-Making Method
Authors: Jin Qichun , Chen Huimin , Hu Fuwen
Published: February 2024

In the wake of Industry 4.0, the ubiquitous internet of things provides big data to potentially quantify the environmental footprint of green products. Further, as the concept of Industry 5.0 emphasizes, the increasing mass customization production makes the product configurations full of individuation and diversification. Driven by these fundamental changes, the design for sustainability of a high-mix low-volume product–service system faces the increasingly deep coupling of technology-driven product solutions and value-driven human-centric goals. The multi-criteria decision making of sustainability issues is prone to fall into the complex, contradictory, fragmented, and opaque flood of information. To this end, this work presents a data-driven quantitative method for the sustainability assessment of product–service systems by integrating analytic hierarchy process (AHP) and data envelopment analysis (DEA) methods to measure the sustainability of customized products and promote the Industry 5.0-enabled sustainable product–service system practice. This method translates the sustainability assessment into a multi-criteria decision-making problem, to find the solution that meets the most important criteria while minimizing trade-offs between conflicting criteria, such as individual preferences or needs and the life cycle sustainability of bespoke products. In the future, the presented method can extend to cover more concerns of Industry 5.0, such as digital-twin-driven recyclability and disassembly of customized products, and the overall sustainability and resilience of the supply chain.
Effects of Diameter Parameters on Gas Flow Field Characteristics in Cyclones: An Experimental Investigation
Authors: Sun Liqiang , Xie Ming , Dong Yingjuan , Li Jiangfei , Song Jianfei
Published: February 2024

The flow field characteristic is crucial for the separation process of cyclones, which includes time–mean and dynamic characteristics. The structural parameters of the cyclone have an important influence on the internal flow field characteristics, among which the cylinder diameter and vortex finder diameter are important structural parameters. This experimental study aimed to assess the effects of diameter parameters on the flow field characteristics of cyclones, especially the dynamic characteristics, which have received less attention in the literature. A hot wire anemometer (HWA) was employed in measuring the instantaneous tangential velocities in cyclones with different cylinder and vortex finder diameters. Time and frequency domain analyses of the measured data revealed that the diameter parameters of cyclones affected not only the distributions of the time–mean and instantaneous tangent velocities but also the intensity and dominant frequency of the instantaneous tangential velocity fluctuations. First, the maximum tangential velocity in the cyclone increased slightly when the cylinder diameter was increased and decreased significantly when the vortex finder diameter was increased. Second, the tangential velocity fluctuation intensity characterized by the standard deviation (Sd) increased on the same dimensionless axial section when the cylinder diameter was increased and the vortex finder diameter was decreased. It was also found that the increases in cylinder diameter and vortex finder diameter led to the dominant frequencies in the cyclone being reduced. Based on the results of this study, the dominant frequency calculation model for cyclones was improved. The conclusions presented in this study may provide valuable insights into the dynamic characteristics of flow fields in cyclones for future improvements to separation performance.
NO and CO Emission Characteristics of Laminar and Turbulent Counterflow Premixed Hydrogen-Rich Syngas/Air Flames
Authors: Cheng Lei , Chen Yanming , Pei Yebin , Sun Guozhen , Zou Jun , Peng Shiyao , Zhang Yang
Published: February 2024

Burning hydrogen-rich syngas fuels derived from various sources in combustion equipment is an effective pathway to enhance energy security and of significant practical implications. Emissions from the combustion of hydrogen-rich fuels have been a main concern in both academia and industry. In this study, the NO and CO emission characteristics of both laminar and turbulent counterflow premixed hydrogen-rich syngas/air flames were experimentally and numerically studied. The results showed that for both laminar and turbulent counterflow premixed flames, the peak NO mole fraction increased as the equivalence ratio increased from 0.6 to 1.0 and decreased as the strain rate increased. Compared with the laminar flames at the same bulk flow velocity, turbulent flames demonstrated a lower peak NO mole fraction but broader NO formation region. Using the analogy theorem, a one-dimensional turbulent counterflow flame model was established, and the numerical results indicated that the small-scale turbulence-induced heat and mass transport enhancements significantly affected NO emission. Considering NO formation at the same level of fuel consumption, the NO formation of the turbulent flame was significantly lower than that of the laminar flame at the same level of fuel consumption, implying that the turbulence-induced heat and mass transfer enhancement favored NOx suppression.
The Clinical Potential of Dimethyltryptamine: Breakthroughs into the Other Side of Mental Illness, Neurodegeneration, and Consciousness
Authors: Colosimo Frankie A. , Borsellino Philip , Krider Reese I. , Marquez Raul E. , Vida Thomas A.
Published: February 2024

The human brain is an extraordinarily complex organ responsible for all aspects of cognition and control. Billions of neurons form connections with thousands of other neurons, resulting in trillions of synapses that create a vast and intricate network. This network is subjected to continuous remodeling that adapts to environmental and developmental changes. The resulting neuroplasticity is crucial to both healthy states and many forms of mental illness and neurodegeneration. This narrative review comprehensively examines N,N-dimethyltryptamine (DMT), a naturally occurring hallucinogen and psychedelic compound, focusing on its implications in promoting neuroplasticity via neuritogenesis. We examine DMT’s pharmacology, including its interaction with serotonergic, sigma-1, and trace amine-associated receptors and their associated signaling pathways. The therapeutic potential of DMT in both animal models and clinical trials is discussed with impacts on perception, cognition, emotion, and consciousness. We uniquely focus on current directions centered on unveiling the direct mechanisms of DMT’s therapeutic effects that demonstrate transformative effects on mental well-being, particularly for conditions like depression, anxiety, and post-traumatic stress disorder. We discuss the connection between DMT and neuroplasticity, offering the potential for forming new neural connections, improving learning, memory, and aiding recovery from brain injuries, including neurorehabilitation and neuroregeneration. The ultimate potential of DMT’s therapeutic efficacy to enhance neurogenesis, especially for neurodegenerative conditions, is also discussed.
Debt: A Political–Theological Device Acting in Favor of the Neoliberal Ethos
Authors: Barros Douglas Ferreira , Barsalini Glauco
Published: February 2024

This article intends to examine debt as a basilar political–theological device acting in favor of the neoliberal ethos. The Papal Encyclical Laudato Si affirms in paragraph 52 that debt today serves the control over the poor peoples in the world. In this article we demonstrate how debt can be seen as a political–theological device that works as an instrument of this specific ethos, aligned with neoliberal principles. We intend to show how these elements are related using three analytical movements. In the first, we present how we understand political theology as a critical reflection about the forms of political power. We observe that power, in its form and in the way it operates, replicates the theological–political power status prior to modernity, operating an excluding inclusion machinery. In the second movement, we analyze the political theology machinery that impacts individuals in the operation of an “excluding inclusion”. Under the political–theological machinery, individuals, groups, or populations are considered as a part of the machinery; they are included because they are incorporated in the new organism as they are excluded from their original content—language, ethos, culture, and their constitution as subjects. Then, in the third, we present the notion of the device, explicitly, a device constituted by a web of odd components and flexible relations that, when isolated as independent elements, act in the subject’s formation. In these terms, debt as a device of the political–theological machinery works to form individuals; it is a device that operates the excluding inclusion to make subjects more and more adapted to the market rules and habits. The very sense of debt in the post-productive era is challenged. We present how the possible exits from this machinery involve not only the debate on the forgiveness of foreign debts, but also how they are intrinsically linked to the creation of a new ethos, new ways of life created by relations outside the orbit of debt control. The conclusion intends to show how necessary it is to restore to people new forms of control over a way of life that is not regulated and ruled by debt. The methodology employed analyzes arguments that originated from works and articles concerning this theme.
The Role of Catholic Life Formation in Fostering Sustainable Environmental Attitudes among Selected Filipino SHS Students
Authors: Baring Rito , Villacrucis Peb Hinojales , Barcenas Jake T. , Arsolon Noel , Dandan Maria Antonette , Foronda Jojit , Legitimas Milver , Pancho Loreto , Ranara Josefina Bernados , Ypanto Dwight Jimenez , Magallon-Avenido Jessica
Published: February 2024

A relevant Religious Education needs to confront the life-threatening global issues humanity faces today. The present study investigates how Catholic Life Formation (CLF) through religious instruction may predict environmental attitudes among students in Cebu City, Philippines. This study analyzes how the following variables may influence Christian environmentalism: students’ reception of CLF inputs, Catholic Social Teachings (CST) perceptions, and students’ beliefs and environmental responses (STB) from a sample of 491 Grade 12 SH students of a private university in Cebu, Philippines. This descriptive quantitative survey used ordinal logistic regression to test our model. We adopted a two-step procedure to gather data: First, an open-ended interview was used to tease the themes and viewpoints of students. Second, incorporating the qualitative output from the interviews, a survey was conducted using a researcher-made self-report paired with the Christian Environmentalism Scale (CES) to describe student attitudes toward the environment. The findings are discussed with respect to Religious Education and CLF environmental advocacy.