bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization and Engineering of a Self-Sufficient CYP102 Enzyme from Bacillus amyloliquefaciens towards Synthesis of In-Chain Hydroxy Fatty Acids
Authors: Zong Li , Zhang Yan , Shao Zhengkang , Wang Yingwu , Guo Zheng , Gao Renjun , Eser Bekir Engin
Published: May 2021

Cytochrome P450 (CYP) mediated enzymatic hydroxylation of fatty acids present a green alternative to chemical synthesis of hydroxy fatty acids (HFAs), which are high-value oleochemicals with various uses in materials industry and medical field. Although many CYPs require the presence of additional reductase proteins for catalytic activity, self-sufficient CYPs have their reductase partner naturally fused into their catalytic domain, leading to a greatly simplified biotransformation process. A recently discovered self-sufficient CYP, BAMF2522 from Bacillus amyloliquefaciens DSM 7, exhibits novel regioselectivity by hydroxylating in-chain positions of palmitic acid generating ω-1 to ω-7 HFAs, a rare regiodiversity profile among CYPs. Besides, F89I mutant of BAMF2522 expanded hydroxylation up to ω-9 position of palmitic acid. Here, we further characterize this enzyme by determining optimum temperature and pH as well as thermal stability. Moreover, using extensive site-directed and site-saturation mutagenesis, we obtained BAMF2522 variants that demonstrate greatly increased regioselectivity for in-chain positions (ω-4 to ω-9) of various medium to long chain fatty acids. Remarkably, when a six-residue mutant was reacted with palmitic acid, 84% of total product content was the sum of ω-7, ω-8 and ω-9 HFA products, the highest in-chain selectivity observed to date with a self-sufficient CYP. In short, our study demonstrates the potential of a recently identified CYP and its mutants for green and sustainable production of a variety of in-chain hydroxy enriched HFAs.
Investigation of Nano-Bio Interactions within a Pancreatic Tumor Microenvironment for the Advancement of Nanomedicine in Cancer Treatment
Authors: Alhussan Abdulaziz , Bromma Kyle , Bozdoğan Ece Pinar Demirci , Metcalfe Andrew , Karasinska Joanna , Beckham Wayne , Alexander Abraham S. , Renouf Daniel J. , Schaeffer David F. , Chithrani Devika B.
Published: May 2021

Pancreatic cancer is one of the deadliest types of cancer, with a five-year survival rate of only 10%. Nanotechnology offers a novel perspective to treat such deadly cancers through their incorporation into radiotherapy and chemotherapy. However, the interaction of nanoparticles (NPs) with cancer cells and with other major cell types within the pancreatic tumor microenvironment (TME) is yet to be understood. Therefore, our goal is to shed light on the dynamics of NPs within a TME of pancreatic origin. In addition to cancer cells, normal fibroblasts (NFs) and cancer-associated fibroblasts (CAFs) were examined in this study due to their important yet opposite roles of suppressing tumor growth and promoting tumor growth, respectively. Gold nanoparticles were used as the model NP system due to their biocompatibility and physical and chemical proprieties, and their dynamics were studied both quantitatively and qualitatively in vitro and in vivo. The in vitro studies revealed that both cancer cells and CAFs take up 50% more NPs compared to NFs. Most importantly, they all managed to retain 70–80% of NPs over a 24-h time period. Uptake and retention of NPs within an in vivo environment was also consistent with in vitro results. This study shows the paradigm-changing potential of NPs to combat the disease.
Integrative Taxonomy Reveals That the Marine Brachyuran Crab Pyromaia tuberculata (Lockington, 1877) Reached Eastern Atlantic
Authors: Lobo-Arteaga Jorge , Tuaty-Guerra Miriam , Gaudêncio Maria José
Published: May 2021

Pyromaia tuberculata is native to the north-eastern Pacific Ocean and currently established in distant regions in the Pacific Ocean and southwest Atlantic. Outside its native range, this species has become established in organically polluted enclosed waters, such as bays. The Tagus estuary, with a broad shallow bay, is one of the largest estuaries in the west coast of Europe, located in western mainland Portugal, bordering the city of Lisbon. In this study, sediment samples were collected in the estuary between 2016 and 2017. Several adult specimens of P. tuberculata, including one ovigerous female, were morphologically and genetically identified, resulting in accurate identification of the species. The constant presence of adults over a 16-month sampling period suggests that the species has become established in the Tagus estuary. Moreover, their short life cycle, which allows for the production of at least two generations per year, with females reaching maturity within six months after settlement, favours population establishment. Despite being referred to as invasive, there are no records of adverse effects of P. tuberculata to the environment and socio-economy in regions outside its native range. However, due to its expanding ability, its inclusion in European monitoring programmes would indeed be desirable.
Multivariate Abundance Analysis of Multi-Host/Multi-Parasite Lungworms in a Sympatric Wild Ruminant Population
Authors: Carrau Tessa , Martínez-Carrasco Carlos , Garijo María Magdalena , Alonso Francisco , de Ybáñez Rocío Ruiz , Tizzani Paolo
Published: May 2021

In the analysis of a multi-host/multi-parasite system and its associated risk factors, it is particularly interesting to understand the natural dynamics among pathogens, their hosts, and the environment in wildlife populations. This analysis is particularly feasible in a scenario where multiple overlapping host populations are present in high densities, along with a complex community of parasites. We aimed to describe and analyze the naturally occurring lungworm polyparasitism in a wild ruminant community in Southeast Spain. The respiratory tracts of 250 specimens belonging to four different species (red deer, mouflon, Iberian ibex, and fallow deer) were studied. Almost half (48.0%) of the animals were infected with bronchopulmonary nematodes. Seven different nematodes were identified of which two genera (Protostrongylus spp. and Dictyocaulus spp.) and three additional species (Cystocaulus ocreatus, Muellerius capillaris, and Neostrongylus linearis) were recorded in at least two ruminants, with the mouflon as the commonest host. Our study shows a significant effect of host species and sampling area, plus a marginal effect of age, on parasite multivariate abundance at the host population level. Mouflon and adults of all hosts appear to carry the highest parasite load on average. From a spatial perspective, the highest parasite abundance was detected at the central part of the park.
The Young Stages of the Cannonball Jellyfish (Stomolophus sp. 2) from the Central Gulf of California (Mexico)
Authors: Gómez-Salinas Laura Cristina , López-Martínez Juana , Morandini André Carrara
Published: May 2021

Exploitation of the cannonball jellyfish (Stomolophus sp. 2) is increasing in Mexico and USA due to successful fisheries associated with seasonal blooms in coastal areas. Previously, it was proposed that such blooms could be identified by recognizing the presence of young stages in the water. In our work, we aim to describe the young stages (ephyra and metaephyra) found in the Las Guásimas lagoon, Sonora, Mexico. The description of specimens is based on photographs, drawings, and morphological measurements aimed at helping in the early detection of blooming events.
Effects of Pandemic-Related School Closures on Pupils’ Performance and Learning in Selected Countries: A Rapid Review
Authors: Zierer Klaus
Published: May 2021

The coronavirus pandemic has led to drastic measures around the world, which have also affected the education system. Schools were closed in the spring of 2020 in almost every country in the world, and many children and young people are still involved in distance learning to this day. What effect these measures have on children’s and young people’s learning performance is important in view of the time pressure under which educational policy decisions have to be taken. The rapid review presented in this paper delivers evidence on the effects of school closures to contain the coronavirus pandemic in the spring of 2020 on children’s and young people’s learning performance in five countries (USA, Belgium, the Netherlands, Switzerland and Germany).
Critical Language Pedagogy and Task-Based Language Teaching: Reciprocal Relationship and Mutual Benefit
Authors: Crookes Graham V. , Ziegler Nicole
Published: May 2021

Curriculum development and empirical research in the field of second language acquisition could benefit the field of critical language pedagogy (CLP) and its practitioners. This article reviews central concepts in the organization of curriculum in CLP and compares them with another major curricular initiative in second language teaching, namely task-based or task-supported approaches, with particular emphasis on task-based language teaching. Content itself (as in activities or materials) and the role of metacognitive instruction are considered. A real-world example of a task-based or task-supported short-term program is reviewed as exemplifying some areas of contact or benefit.
Shared Decision-Making and Information Needs among People with Generalized Anxiety Disorder
Authors: Ramos-García Vanesa , Rivero-Santana Amado , Duarte-Díaz Andrea , Perestelo-Pérez Lilisbeth , Peñate-Castro Wenceslao , Álvarez-Pérez Yolanda , González-González Ana Isabel , Serrano-Aguilar Pedro
Published: May 2021

Shared decision making (SDM) aims to involve patients in the decisions about their care, considering their preferences, values and concerns about the different treatment options. However, research shows that people with mental health problems have considerable unmet information needs about their condition. This community-based cross-sectional study explores the SDM process and information needs among people with Generalized Anxiety Disorder (GAD), as an initial step in the design and development of a Patient Decision Aid for this population. Seventy participants completed an online survey with the Control Preference Scale, and questions about the perceived difficulty of past treatment decisions and the use of the Internet for searching for GAD-related information. Most participants preferred an active (42.9%) or collaborative role (41.4%) in the SDM process, and 53% did not perceive their preferred role. Information provided by healthcare professionals was considered insufficient by 28% of the sample, and over 30% reported using the Internet to look for GAD-related information at least once a week or more. The most relevant GAD-related information needs were general information (71.4%), information on self-help groups (65.7%), recommendations on how to face this disorder (61.4%) and information on treatment options (50%). Exploratory analyses showed that patients who perceived an active participation were more likely to search for information frequently (p = 0.038), and those who felt more involved than desired tended to search for more themes (p = 0.049). In summary, the study showed that a considerable percentage of GAD patients have unmet needs related to decision-making participation and information.
Context-Based Inter Mode Decision Method for Fast Affine Prediction in Versatile Video Coding
Authors: Jung Seongwon , Jun Dongsan
Published: May 2021

Versatile Video Coding (VVC) is the most recent video coding standard developed by Joint Video Experts Team (JVET) that can achieve a bit-rate reduction of 50% with perceptually similar quality compared to the previous method, namely High Efficiency Video Coding (HEVC). Although VVC can support the significant coding performance, it leads to the tremendous computational complexity of VVC encoder. In particular, VVC has newly adopted an affine motion estimation (AME) method to overcome the limitations of the translational motion model at the expense of higher encoding complexity. In this paper, we proposed a context-based inter mode decision method for fast affine prediction that determines whether the AME is performed or not in the process of rate-distortion (RD) optimization for optimal CU-mode decision. Experimental results showed that the proposed method significantly reduced the encoding complexity of AME up to 33% with unnoticeable coding loss compared to the VVC Test Model (VTM).
Photosynthesis Traits of Pioneer Broadleaves Species from Tailing Dumps in Călimani Mountains (Eastern Carpathians)
Authors: Popa Andrei , Popa Ionel
Published: May 2021

The reforestation and stable ecological restoration of tailings dumps resulting from surface mining activities in the Călimani Mountains represent an ongoing environmental challenge. To assess the suitability of different tree species for restoration efforts, photosynthetic traits were monitored in four broadleaf pioneer species—green alder (Alnus alnobetula (Ehrh.) K. Koch), aspen (Populus tremula L.), silver birch (Betula pendula Roth.), and goat willow (Salix caprea L.)—that naturally colonized the tailings dumps. Green alder and birch had the highest photosynthetic rate, followed by aspen and goat willow. Water use efficiency parameters (WUE and iWUE) were the highest for green alder and the lowest for birch, with intermediary values for aspen and goat willow. Green alder also exhibited the highest carboxylation efficiency, followed by birch. During the growing season, net assimilation and carboxylation efficiency exhibited a maximum in late July and a minimum in late June. The key limitation parameters of the photosynthetic process derived from the FvCB model (Vcmax and Jmax) were the highest for green alder and exhibited a maximum in late July, regardless of the species. Based on photosynthetic traits, the green alder—a woody N2-fixing shrub—is the most well-adapted and photosynthetically efficient species that naturally colonized the tailings dumps in the Călimani Mountains.
Photosynthetic Acclimation and Growth Responses to Elevated CO2 Associate with Leaf Nitrogen and Phosphorus Concentrations in Mulberry (Morus multicaulis Perr.)
Authors: Shi Songmei , Xu Xiao , Dong Xingshui , Xu Chenyang , Qiu Yuling , He Xinhua
Published: May 2021

Mulberry (Morus spp.) is a multipurpose tree that is worldwide planted because of its economic importance. This study was to investigate the likely consequences of anticipated future elevated CO2 (eCO2) on growth, physiology and nutrient uptake of nitrogen (N), phosphorus (P) and potassium (K) in two most widely cultivated mulberry (Morus multicaulis Perr.) varieties, QiangSang-1 and NongSang-14, in southwest China. A pot experiment was conducted in environmentally auto-controlled growth chambers under ambient CO2 (ACO2, 410/460 ppm, daytime/nighttime) and eCO2 (710/760 ppm). eCO2 significantly increased plant height, stem diameter, leaf numbers and biomass production, and decreased chlorophyll concentrations, net photosynthetic rate, stomatal conductance and transpiration rate of these two mulberry varieties. Under eCO2 leaf N and P, and root N, P and K concentrations in both mulberry varieties decreased, while plant total P and K uptake in both varieties were enhanced, and an increased total N uptake in NongSang-4, but not in QiangSang-1. Nutrient dilution and transpiration rate were the main factors driving the reduction of leaf N and P, whereas changes in plant N and P demand had substantial impacts on photosynthetic inhibition. Our results can provide effective nutrient management strategies for a sustainable mulberry production under global atmosphere CO2 rising scenarios.
Assessing the Broader Value of Planted Forests to Inform Forest Management Decisions
Authors: Yao Richard T. , Palmer David J. , Payn Tim W. , Strang Sally , Maunder Colin
Published: May 2021

This study highlights the importance of incorporating objectively quantified, non-market environmental values (such as avoided erosion and carbon sequestration) into land use decision making for sustainable forest management. A continuously developing approach that has facilitated discussions between researchers, industries, and governments on the quantification of non-market values is the ecosystem services (ES) framework. Using a spatial economic tool, called Forest Investment Framework, this study is, to the best of our knowledge, the first assessment of the market (timber) and non-market (carbon sequestration, avoided nitrogen leaching and avoided erosion) ES values of the 1.75 million-hectare New Zealand planted forest estate. To collect the views of key planted forest industry representatives on ES assessment/quantification, we interviewed 14 forest managers representing 60% of the planted forest area. Results from the spatial economic analysis indicated that the non-market ES values can be more than four times the timber profit nationally, and up to 12 times higher in New Zealand’s most erosion-prone region. These estimated values are indicative and should be treated with caution. From a sensitivity analysis, we found that different discount rates significantly impact ES values, ratios, and distributions. Results from the interviews indicated that ES quantification helped inform decision making by supporting license to operate, while also signaling the development of a reward system for sustaining ES. Sixty-four percent of survey respondents identified the importance of quantifying ES in ecological terms and describing other non-market ES in spatial, qualitative, or binary forms. Overall, this study provided evidence of how estimated non-market ES values compare with market values and highlighted the importance of including them in decision making processes. Future cost benefit analyses that incorporate these non-market monetary ES values would complement multi-criteria analysis that integrate additional dimensions and allow decision makers to rank options based on their particular criteria.
Hadamard-Type Fractional Heat Equations and Ultra-Slow Diffusions
Authors: De Gregorio Alessandro , Garra Roberto
Published: May 2021

In this paper, we study diffusion equations involving Hadamard-type time-fractional derivatives related to ultra-slow random models. We start our analysis using the abstract fractional Cauchy problem, replacing the classical time derivative with the Hadamard operator. The stochastic meaning of the introduced abstract differential equation is provided, and the application to the particular case of the fractional heat equation is then discussed in detail. The ultra-slow behaviour emerges from the explicit form of the variance of the random process arising from our analysis. Finally, we obtain a particular solution for the nonlinear Hadamard-diffusive equation.
Gender Differences in Repeated Dishonest Behavior: Experimental Evidence
Authors: Chowdhury Subhasish M. , Jeon Joo Young , Kim Chulyoung , Kim Sang-Hyun
Published: May 2021

We investigate gender differences in lying behavior when the opportunity to tell lies is repeated. In specific, we distinguish the situations in which such an opportunity can be planned versus when it comes as a surprise. We utilize data from an existing published research and show that when the opportunity to tell a lie comes as a surprise, then on the first occasion, males lie more than females. However, when telling lies can be planned, then there is no gender difference in telling a lie. When planning is possible, females tell more lies in the first occasion compared to when it is not possible to plan; males do not show such behavior. On the second and final occasion, males tell more lies than females when they either could not plan but had the opportunity to a lie before, or could plan but did not have to tell a lie before. These observations can be interpreted in terms of the gender differences in consistent versus compensatory moral behavior.
Climate Change Sustainability: From Bargaining to Cooperative Balanced Approach
Authors: Ciano Tiziana , Ferrara Massimiliano , Gangemi Mariangela , Merenda Domenica Stefania , Pansera Bruno Antonio
Published: May 2021

This work aims to provide different perspectives on the relationships between cooperative game theory and the research field concerning climate change dynamics. New results are obtained in the framework of competitive bargaining solutions and related issues, moving from a cooperative approach to a competitive one. Furthermore, the dynamics of balanced and super-balanced games are exposed, with particular reference to coalitions. Some open problems are presented to aid future research in this area.
Influences of Differing Menarche Status on Motor Capabilities of Girls, 13 To 16 Years: A Two-Year Follow-Up Study
Authors: Gerber Barry , Pienaar Anita E. , Kruger Ankebe
Published: May 2021

Puberty and the onset of menarche influences the motor performance of girls. However, the magnitude of these influences during varying maturity status, is not clear. This longitudinal study over two years aimed to investigate differences in motor fitness between early and late developing girls based on pre- and post-menarche status. A convenience sample (n = 58) of girls aged 13.51 ± 3.51, divided by means of the Status Quo method into pre (n = 13) and post-menarche (n = 45) groups, was used. Motor fitness was tested once annually by standardized protocols. Basic statistics, independent t-testing and a repeated measures ANOVA with a post hoc Bonferonni correction were used (p < 0.05 = statistical significance). Effect sizes were determined by Cohen’s d-values. Only explosive upper body strength differed significantly between groups during baseline, favoring post-menarche girls. Initially, post-menarche girls showed advantages in hand-eye coordination and speed (p > 0.05) with pre-menarche girls performing better in agility and explosive leg strength (p > 0.05). At 15.51 years, no significant, between-group differences were found. Pre-menarche girls surpassed post-menarche girls in hand-eye coordination and 0–40 m speed and post-menarche girls displayed higher explosive leg and upper body strength scores (p > 0.05). Our data show that the potential to excel in sport based on motor capabilities can only be accurately estimated 1–2 years after reaching menarche.
Space-Time Cluster’s Detection and Geographical Weighted Regression Analysis of COVID-19 Mortality on Texas Counties
Authors: Zhang Jinting , Wu Xiu , Chow T. Edwin
Published: May 2021

As COVID-19 run rampant in high-density housing sites, it is important to use real-time data in tracking the virus mobility. Emerging cluster detection analysis is a precise way of blunting the spread of COVID-19 as quickly as possible and save lives. To track compliable mobility of COVID-19 on a spatial-temporal scale, this research appropriately analyzed the disparities between spatial-temporal clusters, expectation maximization clustering (EM), and hierarchical clustering (HC) analysis on Texas county-level. Then, based on the outcome of clustering analysis, the sensitive counties are Cottle, Stonewall, Bexar, Tarrant, Dallas, Harris, Jim hogg, and Real, corresponding to Southeast Texas analysis in Geographically Weighted Regression (GWR) modeling. The sensitive period took place in the last two quarters in 2020 and the first quarter in 2021. We explored PostSQL application to portray tracking Covid-19 trajectory. We captured 14 social, economic, and environmental impact’s indices to perform principal component analysis (PCA) to reduce dimensionality and minimize multicollinearity. By using the PCA, we extracted five factors related to mortality of COVID-19, involved population and hospitalization, adult population, natural supply, economic condition, air quality or medical care. We established the GWR model to seek the sensitive factors. The result shows that adult population, economic condition, air quality, and medical care are the sensitive factors. Those factors also triggered high increase of COVID-19 mortality. This research provides geographical understanding and solution of controlling COVID-19, reference of implementing geographically targeted ways to track virus mobility, and satisfy for the need of emergency operations plan (EOP).
The Organizational Atmosphere in Israeli Hospital during COVID-19: Concerns, Perceptions, and Burnout
Authors: Bashkin Osnat , Davidovitch Nadav , Asna Noam , Schwartz Doron , Dopelt Keren
Published: May 2021

The COVID-19 crisis poses challenges to healthcare systems and requires micro- and macro-organizational adaptations. This study examined the organizational atmosphere in Israeli hospitals by evaluating workers’ perceptions and concerns about the COVID-19 crisis and its management. At the end of the pandemic’s first wave in Israel, 547 healthcare workers responded to an online survey, which inquired about COVID-19 concerns at the individual and family level, perceptions at the national and organizational level, perceptions of the way the crisis was managed, self-assessment of coping with the crisis and burnout, and demographics. Findings showed that healthcare workers expressed deep concerns for family members and apprehension at a national level. Respondents noted that they were coping well with the crisis while expressing negative perceptions of how the crisis was managed. A regression model showed that the low self-assessment of medical staff of coping with the crisis, deep concerns at the organizational level, negative perceptions of crisis management, and providing care for COVID-19 patients were predictors of burnout. The findings emphasize the importance of developing a supportive organizational culture for hospital workers. Awareness of their concerns and perceptions is essential to improve organizational culture and healthcare systems’ ability to continue fighting the virus and confront future health crises.
Mortality Risk from Respiratory Diseases Due to Non-Optimal Temperature among Brazilian Elderlies
Authors: Jacobson Ludmilla da Silva Viana , Oliveira Beatriz Fátima Alves de , Schneider Rochelle , Gasparrini Antonio , Hacon Sandra de Souza
Published: May 2021

Over the past decade, Brazil has experienced and continues to be impacted by extreme climate events. This study aims to evaluate the association between daily average temperature and mortality from respiratory disease among Brazilian elderlies. A daily time-series study between 2000 and 2017 in 27 Brazilian cities was conducted. Data outcomes were daily counts of deaths due to respiratory diseases in the elderly aged 60 or more. The exposure variable was the daily mean temperature from Copernicus ERA5-Land reanalysis. The association was estimated from a two-stage time series analysis method. We also calculated deaths attributable to heat and cold. The pooled exposure–response curve presented a J-shaped format. The exposure to extreme heat increased the risk of mortality by 27% (95% CI: 15–39%), while the exposure to extreme cold increased the risk of mortality by 16% (95% CI: 8–24%). The heterogeneity between cities was explained by city-specific mean temperature and temperature range. The fractions of deaths attributable to cold and heat were 4.7% (95% CI: 2.94–6.17%) and 2.8% (95% CI: 1.45–3.95%), respectively. Our results show a significant impact of non-optimal temperature on the respiratory health of elderlies living in Brazil. It may support proactive action implementation in cities that have critical temperature variations.
Exploring the Association between Sleep Quality and Heart Rate Variability among Female Nurses
Authors: Hsu Hsiu-Chin , Lee Hsiu-Fang , Lin Mei-Hsiang
Published: May 2021

The quality of nurses’ work has a direct effect on patient health, and poor sleep has been positively associated with nurses’ medical errors. The aim of this study was to investigate the relationship between quality of sleep and heart rate variability (HRV) among female nurses. A descriptive cross-sectional correlational study design was used in January 2014 to study female nurses (n = 393) employed in a medical center in Taiwan. Data were obtained from several questionnaires. HRV was analyzed with five-minute recordings of heart rate signals obtained using a Heart Rater SA-3000P. Approximately 96% of the participants self-reported a poor quality of sleep. Compared to non-shift nurses, significant decreases were found in total power (TP) and low-frequency HRV among shift-work nurses. However, negative correlations were found between sleep quality and HRV, including total power, low frequency, and the low frequency/high frequency ratio (r = −0.425, p < 0.05; r = −0.269, −0.266, p < 0.05). In a stepwise multiple regression analysis, 23.1% of variance in quality of sleep can be explained by TP and heart rate. The sleep quality of female nurses was poor and this affected their autonomic nervous system, which can contribute unfavorable consequences for their health.
Health and Risk Behaviors of Bystanders: An Integrative Theoretical Model of Bystanders’ Reactions to Mistreatment
Authors: Itzkovich Yariv , Barhon Ella , Lev-Wiesel Rachel
Published: May 2021

This article constructs a comprehensive theoretical model that outlines bystanders’ emotional and behavioral responses to the mistreatment of adolescent peers. The model captures bystanders’ risk and health risk behaviors, which have been overlooked in the context of their reactions; when addressed at all in connection with bystanders of bullying among adolescents, they have been treated separately. Here, we present bystanders’ emotional and cognitive reactions and their impact on bystanders’ responses including a set of responses that demonstrate risk and health risk behaviors that are directed to the bystander as a victim by proxy. The theoretical framework is the conservation of resources theory, which posits that personal resources (i.e., potency and moral disengagement) and social resources impact the process that leads to bystanders’ reactions. Previous models have overlooked the integrative viewpoint of bystanders, and comprehensive models that explain bystanders’ behavioral and emotional responses have received little attention especially with regards to adolescents. Two recent models overlooked core features embedded in the current model, including the risk and health risk behaviors that it integrates. The proposed model presents a novel and more comprehensive view of bystanders’ reactions and the process underlying these reactions. It integrates existing knowledge embedded in other existing models. At the same time, this perspective indicates the centricity of potency as a key resource that dictates the emotional response and behaviors of bystanders. This potentially allows for new applications in the mitigation of adverse impacts that follow the witnessing of mistreatment. The article discusses these applications, which are based on previous findings, their implications for practice, and directions for future empirical research necessary to validate the model.
Space–Time Clustering Characteristics of Malaria in Bhutan at the End Stages of Elimination
Authors: Wangdi Kinley , Penjor Kinley , Tobgyal  , Lawpoolsri Saranath , Price Ric N. , Gething Peter W. , Gray Darren J. , Da Silva Fonseca Elivelton , Clements Archie C. A.
Published: May 2021

Malaria in Bhutan has fallen significantly over the last decade. As Bhutan attempts to eliminate malaria in 2022, this study aimed to characterize the space–time clustering of malaria from 2010 to 2019. Malaria data were obtained from the Bhutan Vector-Borne Disease Control Program data repository. Spatial and space–time cluster analyses of Plasmodium falciparum and Plasmodium vivax cases were conducted at the sub-district level from 2010 to 2019 using Kulldorff’s space–time scan statistic. A total of 768 confirmed malaria cases, including 454 (59%) P. vivax cases, were reported in Bhutan during the study period. Significant temporal clusters of cases caused by both species were identified between April and September. The most likely spatial clusters were detected in the central part of Bhutan throughout the study period. The most likely space–time cluster was in Sarpang District and neighboring districts between January 2010 to June 2012 for cases of infection with both species. The most likely cluster for P. falciparum infection had a radius of 50.4 km and included 26 sub-districts with a relative risk (RR) of 32.7. The most likely cluster for P. vivax infection had a radius of 33.6 km with 11 sub-districts and RR of 27.7. Three secondary space–time clusters were detected in other parts of Bhutan. Spatial and space–time cluster analysis identified high-risk areas and periods for both P. vivax and P. falciparum malaria. Both malaria types showed significant spatial and spatiotemporal variations. Operational research to understand the drivers of residual transmission in hotspot sub-districts will help to overcome the final challenges of malaria elimination in Bhutan.
Physical Activity during the First Lockdown of the COVID-19 Pandemic: Investigating the Reliance on Digital Technologies, Perceived Benefits, Barriers and the Impact of Affect
Authors: Symons Michelle , Meira Cunha Carmem , Poels Karolien , Vandebosch Heidi , Dens Nathalie , Alida Cutello Clara
Published: May 2021

The measures to fight the spread of the COVID-19 pandemic have been concentrated on inviting people to stay at home. This has reduced opportunities to exercise while also shedding some light on the importance of physical health. Based on an online survey, this paper investigated physical activity behaviours of a Belgians sample (n = 427) during the lockdown period between the end of May 2020 and the beginning of June 2020 and found that, during this period, the gap between sufficiently and insufficiently active individuals widened even more. This paper analysed important moderators of physical activity behaviours, such as barriers and benefits to exercise, digital support used to exercise, and individuals’ emotional well-being. Descriptive analysis and analyses of variance indicated that, generally, individuals significantly increased their engagement in exercise, especially light- and moderate-intensity activities, mostly accepted the listed benefits but refused the listed barriers, increased their engagement in digital support and did not score high on any affective measures. A comparison between sufficiently active and insufficiently active individuals during the lockdown showed that the former engaged even more in physical activity, whereas the latter exercised equally (i.e., not enough) or even less compared to before the lockdown. By means of a logistic regression, five key factors of belonging to the sufficiently active group were revealed and discussed. Practical implications for government and policies are reviewed.
Place Attachment and Household Disaster Preparedness: Examining the Mediation Role of Self-Efficacy
Authors: Wang Ziyi , Han Ziqiang , Liu Lin , Yu Shaobin
Published: May 2021

Household preparedness is essential for resilience-building and disaster risk reduction. Limited studies have explored the correlations between place attachment, self-efficacy, and disaster preparedness, especially in the east Asian cultural context. This study investigates the mediating role of self-efficacy between place attachment and disaster preparedness based on data from the 2018 Shandong General Social Survey (N = 2181) in China. We categorized the preparedness behaviors into three specific clusters: material, behavioral and awareness preparedness. Multiple linear regressions and the Sobel Goodman tests were employed to estimate the correlations with the control of necessary confounding variables such as disaster experience, socioeconomic and demographic characteristics. The results demonstrate that both the place attachment and self-efficacy are correlated with higher degrees of overall preparedness and all three types of preparedness, and self-efficacy plays a mediating role between place attachment and disaster preparedness. These findings highlight the importance of promoting place attachment and self-efficacy in the advocacies and outreach activities of disaster preparedness.
Quantifying the Effects of Social Distancing on the Spread of COVID-19
Authors: Daghriri Talal , Ozmen Ozlem
Published: May 2021

This paper studies the interplay between social distancing and the spread of the COVID-19 disease—a global pandemic that has affected most of the world’s population. Our goals are to (1) to observe the correlation between the strictness of social distancing policies and the spread of disease and (2) to determine the optimal adoption level of social distancing policies. The earliest instances of the virus were found in China, and the virus has reached the United States with devastating consequences. Other countries severely affected by the pandemic are Brazil, Russia, the United Kingdom, Spain, India, Italy, and France. Although it is impossible to stop it, it is possible to slow down its spread to reduce its impact on the society and economy. Governments around the world have deployed various policies to reduce the virus spread in response to the pandemic. To assess the effectiveness of these policies, the system’s dynamics of the society needs to be analyzed, which is generally not possible with mathematical linear equations or Monte Carlo methods because human society is a complex adaptive system with continuous feedback loops. Because of the challenges with the other methods, we chose agent-based methods to conduct our study. Moreover, recent agent-based modeling studies for the COVID-19 pandemic show significant promise in assisting decision-makers in managing the crisis by applying policies such as social distancing, disease testing, contact tracing, home isolation, emergency hospitalization, and travel prevention to reduce infection rates. Based on modeling studies conducted in Imperial College, increasing levels of interventions could slow the spread of disease and infection. We ran the model with six different percentages of social distancing while keeping the other parameters constant. The results show that social distancing affects the spread of COVID-19 significantly, in turn decreasing the spread of disease and infection rates when implemented at higher levels. We also validated these results by using the behavior space tool with ten experiments with varying social distancing levels. We conclude that applying and increasing social distancing policy levels leads to a significant reduction in infection spread and the number of deaths. Both experiments show that infection rates are reduced drastically when social distancing intervention is implemented between 80% to 100%.
Implementation of a Sulfide–Air Fuel Cell Coupled to a Sulfate-Reducing Biocathode for Elemental Sulfur Recovery
Authors: Blázquez Enric , Gabriel David , Baeza Juan Antonio , Guisasola Albert , Ledezma Pablo , Freguia Stefano
Published: May 2021

Bio-electrochemical systems (BES) are a flexible biotechnological platform that can be employed to treat several types of wastewaters and recover valuable products concomitantly. Sulfate-rich wastewaters usually lack an electron donor; for this reason, implementing BES to treat the sulfate and the possibility of recovering the elemental sulfur (S0) offers a solution to this kind of wastewater. This study proposes a novel BES configuration that combines bio-electrochemical sulfate reduction in a biocathode with a sulfide–air fuel cell (FC) to recover S0. The proposed system achieved high elemental sulfur production rates (up to 386 mg S0-S L−1 d−1) with 65% of the sulfate removed recovered as S0 and a 12% lower energy consumption per kg of S0 produced (16.50 ± 0.19 kWh kg−1 S0-S) than a conventional electrochemical S0 recovery system.
Incidence and Related Factors of Infidelity among Medical Doctors and Nurses
Authors: Guerrero Sara , Castro-Luna Gracia , Zapata Boluda Rosa , Freites Aida , García Rafael , Parrón-Carreño Tesifón
Published: May 2021

Although there is a large body of research addressing infidelity, no study, to our knowledge, has specifically addressed infidelity in doctors and nurses and the correlation with work hours, schedule and other variables. This research aimed to know the incidence of and factors related to infidelity among doctors and nurses. A descriptive study was carried out, studying the association of certain variables. In total, 367 volunteer participants completed an online survey. Of them, 21% either have or have had an unfaithful relationship. The majority (81.7%) were doctors. Men were 4.3 times more unfaithful than women, with these differences being statistically significant (OR = 4.37, p < 0.001). Of the participants involved in an unfaithful relationship within the work area, the majority were men. Likewise, those who reported having had sex in the doctor’s room on duty were also men, with these differences being statistically significant (OR = 12.81, p < 0.01). The night emergency schedule was 60% more frequent in unfaithful people, and these differences were statistically significant (OR = 12.43, p < 0.01). There is a significant rate of infidelity in doctors and nurses. Men are more likely to be unfaithful than women are, and people who work nighttime emergencies are more likely to be unfaithful.
Personal Variables of Protection against Cannabis Use in Adolescence: The Roles of Emotional Intelligence, Coping Styles, and Assertiveness as Associated Factors
Authors: González-Yubero Sara , Lázaro-Visa Susana , Palomera Raquel
Published: May 2021

(1) Background: Many public bodies have warned of the increased consumption of cannabis, particularly among adolescents. From the Positive Youth Development approach, the promotion of personal protective factors takes on special importance against some risks, such as the consumption of addictive substances. (2) Methods: This research is one of the first to study the role of trait and ability emotional intelligence in relation to cannabis use and with respect to other personal variables of protection, such as coping styles and assertiveness. For this purpose, a final sample of 799 schoolchildren was obtained. (3) Results: After controlling for age and gender, the results of the regression analyses revealed that emotional perception, emotional facilitation, emotional clarity, emotional repair, active coping style, and assertiveness were inversely and significantly associated with cannabis use behaviors. On the other hand, the emotional attention and avoidant coping style factors were positively and significantly associated with these behaviors. (4) Conclusions: These findings provide new evidence that could be useful in terms of guiding health-promoting clinical and educational interventions at an early age.
Associations between Self-Esteem, Psychological Stress, and the Risk of Exercise Dependence
Authors: Wågan Frida Austmo , Darvik Monica Dahle , Pedersen Arve Vorland
Published: May 2021

Body concerns and stress-related disorders are increasing in the younger population in a wide range of nations. Studies find links between both self-worth, exercise dependence, and self-esteem in relation to stress, but few have considered all three variables in relation to one another. The present study explored whether the co-appearance of high levels of psychological distress, and low levels of self-esteem may be a vulnerability factor for developing exercise dependence by studying the links between self-esteem, psychological stress, and exercise dependence. A standardized cross-sectional questionnaire was completed by 203 regular exercisers attending two gyms (mean age: 35.9 years). The variables self-esteem, psychological distress, and exercise dependence were all significantly correlated with each other, even after weekly exercise amount, age, and gender had been accounted for. Those who exercised for more than 9 h per week had a significantly higher score on stress and exercise dependence symptoms, and a lower score on self-esteem compared with the remaining groups. One could hypothesize that low self-esteem is a vulnerability factor and high psychological stress a maintenance factor for an exercise-dependent person. It is argued that more focus should be directed toward the negative consequences of excessive exercise.
The Relationship between Reactive Balance Control and Back and Hamstring Strength in Physiotherapists with Non-Specific Back Pain: Protocol for a Cross-Sectional Study
Authors: Zemková Erika , Ďurinová Eva , Džubera Andrej , Horníková Henrieta , Chochol Juraj , Koišová Jana , Šimonová Michaela , Zapletalová Ludmila
Published: May 2021

Back pain is one of the most costly disorders among the worldwide working population. Within that population, healthcare workers are at a high risk of back pain. Though they often demonstrate awkward postures and impaired balance in comparison with healthy workers, there is no clear relationship between compensatory postural responses to unpredictable stimuli and the strength of related muscle groups, in particular in individuals with mild to moderate back pain. This paper presents a study protocol that aims to evaluate the relationship between peak anterior to peak posterior displacements of the center of pressure (CoP) and corresponding time from peak anterior to peak posterior displacements of the CoP after sudden external perturbations and peak force during a maximum voluntary isometric contraction of the back and hamstring muscles in physiotherapists with non-specific back pain in its early stages. Participants will complete the Oswestry Disability Questionnaire. Those that rate their back pain on the 0–10 Low Back Pain Scale in the ranges 1–3 (mild pain) and 4–6 (moderate pain) will be considered. They will undergo a perturbation-based balance test and a test of the maximal isometric strength of back muscles and hip extensors. We assume that by adding tests of reactive balance and strength of related muscle groups in the functional testing of physiotherapists, we would be able to identify back problems earlier and more efficiently and therefore address them well before chronic back disorders occur.
Acceptability of COVID-19 Vaccines and Protective Behavior among Adults in Taiwan: Associations between Risk Perception and Willingness to Vaccinate against COVID-19
Authors: Tsai Feng-Jen , Yang Hsiu-Wen , Lin Chia-Ping , Liu Jeffrey Zen
Published: May 2021

This study aims to evaluate acceptance of COVID-19 vaccines and the impact of risk perception on vaccine acceptance and personal health protective behaviors in Taiwan. A nationwide cross-sectional study was conducted from 19 to 30 October 2020; 1020 participants were included in the final analysis; chi-square and logistic regression analyses were conducted. In total, 52.7% of participants were willing to receive COVID-19 vaccines, 63.5% perceived the severity of COVID-19 in Taiwan as “not serious”, and nearly 40% were worried about COVID-19 infection. Participants with higher perceived severity of COVID-19 had significantly higher odds of refusing the vaccine (OR = 1.546), while those worried about infection had lower odds of poor health protective behaviors (OR = 0.685). Vaccine refusal reasons included “the EUA process is not strict enough” (48.7%) and “side effects” (30.3%). Those who had previously refused other vaccinations were 2.44 times more likely to refuse the COVID-19 vaccines. Participants’ age had an influence on COVID-19 vaccine acceptance. In general, the Taiwanese public’s acceptance of the vaccine was lower than that in other high-income countries. Elderly participants and those with college-level education and above who had previously refused vaccines had lower willingness to receive a COVID-19 vaccine. Risk perception was positively associated with personal health protective behaviors but negatively associated with COVID-19 vaccine acceptance.