bluuebunny/crossref_metadata_2025 huggingface dataset:
Osteosarcoma Cell-Derived Exosomal ELFN1-AS1 Mediates Macrophage M2 Polarization via Sponging miR-138-5p and miR-1291 to Promote the Tumorgenesis of Osteosarcoma
Authors: Wang Bangmin , Wang Xin , Li Po , Niu Xiaoying , Liang Xiaoxiao , Liu Guancong , Liu Zhiyong , Ge Hong
Published: June 2022

Background

Exosomes play an important role in cell-cell communication by transferring genetic materials such as long non-coding RNAs (lncRNAs) between cancer cells and tumor-associated macrophages (TAMs) in the tumor microenvironment (TME). Recent studies revealed that lncRNA ELFN1-AS1 could function as an oncogene in many human cancers. However, the role of extracellular lncRNA ELFN1-AS1 in cell-to-cell communication of osteosarcoma (OS) has not been fully investigated.

Methods

Functional studies, including CCK-8, EdU staining and transwell assay were performed to investigate the role of ELFN1-AS1 in the progression of OS. 143B via xenograft mouse model was established to assess the role of ELFN1-AS1 in vivo. In addition, transmission electron microscopy (TEM) and real-time quantitative PCR (RT-qPCR) assay were used to verify the existence of exosomal ELFN1-AS1.

Results

The level of ELFN1-AS1 was markedly upregulated in patients with advanced OS and in OS cells. In addition, overexpression of ELFN1-AS1 significantly promoted the proliferation, migration and invasion of OS cells, while knockdown of ELFN1-AS1 exhibited the opposite effects. Meanwhile, ELFN1-AS1 could be transferred from OS cells to macrophages via exosomes. Exosomal ELFN1-AS1 from 143B cells was able to promote macrophage M2 polarization, and M2 macrophage in return facilitated OS progression. Mechanistically, overexpression of ELFN1-AS1 upregulated CREB1 level via sponging miR-138-5p and miR-1291 in macrophage via.

Conclusion

OS cell-derived exosomal ELFN1-AS1 was able to induce macrophage M2 polarization via sponging miR-138-5p and miR-1291, and M2 macrophage notably facilitated the progression of OS. These data suggested that ELFN1-AS1 might serve as a potential therapeutic target for osteosarcoma.
The Anti-Tumor Efficacy of Verbascoside on Ovarian Cancer via Facilitating CCN1-AKT/NF-κB Pathway-Mediated M1 Macrophage Polarization
Authors: Ren Yu , He Jinying , Zhao Wenhua , Ma Yuzhen
Published: June 2022

Background

Ovarian cancer (OC) is the leading cause of gynecological cancer-related mortality. Verbascoside (VB) is a phenylpropanoid glycoside from Chinese herbs, with anti-tumour activities. This study aimed to investigate the effects and mechanism of VB on OC.

Methods

OC cell lines SKOV3 and A2780 were used in this study. Cell viability, proliferation, and migration were measured using CCK-8, clonogenic, and transwell assays, respectively. Apoptosis and M1/M2 macrophages were detected using flow cytometry. The interaction between VB and CCN1 was predicted by molecular docking. The mRNA expression of CCN1 was detected by RT-qPCR. The protein levels of CCN1, AKT, p-AKT, p65, and p-p65 were determined by western blotting. A xenograft mice model was established for in vivo validation.

Results

VB inhibited OC cell proliferation and migration in a dose-dependent manner, and promoted apoptosis and M1 macrophage polarization. VB downregulated CCN1 and inhibited the AKT/NF-κB pathway. LY294002, an AKT inhibitor, potentiated the anti-tumour effects of VB. CCN1 overexpression weakened the anti-tumour effects of VB and VB + LY294002. In vivo experiments verified that VB inhibited tumour growth and promoted M1 polarization, which is regulated by the CCN1-mediated AKT/NF-κB pathway.

Conclusion

VB triggers the CCN1-AKT/NF-κB pathway-mediated M1 macrophage polarization for protecting against OC.
A Comprehensive Pan-Cancer Analysis of the Tumorigenic Role of Matrix Metallopeptidase 7 (MMP7) Across Human Cancers
Authors: Meng Nana , Li Yaguang , Jiang Pengcheng , Bu Xuefeng , Ding Jifei , Wang Yan , Zhou Xiaodong , Yu Feng , Zhang Yongjun , Zhang Jie , Xia Leizhou
Published: June 2022

Growing evidence has shown the oncogenic function of matrix metallopeptidase 7 (MMP7) in various tumors. However, no systemic pan-cancer analysis on the association between MMP7 and different cancers based on big clinical data is available. TIMER2, GEPIA2, UALCAN, cBioPortal, String, Metascape, and other web databases were searched in the present study. Generally, MMP7 expression is significantly upregulated in most The Cancer Genome Atlas (TCGA) cancer types compared to the paired normal controls, yet is downregulated in tumor tissues of invasive breast carcinoma (BRCA), kidney chromophobe (KICH), kidney renal clear cell carcinoma (KIRC), liver hepatocellular carcinoma (LIHC), and skin cutaneous melanoma (SKCM). MMP7 protein expression is notably higher in the primary tumor tissues of colon cancer, lung adenocarcinoma (LUAD), and uterine corpus endometrial carcinoma (UCEC) than in normal tissues and is significantly lower in the primary tumor tissues of breast cancer, clear cell renal carcinoma, and ovarian cancer. Furthermore, MMP7 expression is strongly associated with pathological stages, clinical outcomes, tumor mutational burden (TMB), and microsatellite instability (TSI). Gene amplification was detected in most TCGA cancer types. In addition, the missense mutation is the primary type of MMP7 genetic alteration in tumors. Significant positive correlations between MMP7 expression and cancer-associated fibroblasts (CAFs) have been demonstrated in most TCGA cancers. MMP7 expression was also found to be positively correlated with infiltration of dendritic cells and macrophages in some specific tumor types. Functional enrichment analysis by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and gene ontology (GO) methods revealed that RNA processing and DNA damage checkpoints might reveal the pathogenetic mechanisms of MMP7. This pan-cancer analysis provides a clear panorama for the tumorigenic roles of MMP7 across different cancer types. Moreover, MMP7 could be a potential drug therapeutic target in such cancers.
The Disease Spectrum and Influencing Factors of NICU in Xiangxi, Underdeveloped Area of China: A 9-Year Retrospective Study
Authors: Xie Fen , Zhu Yuhua , Chen Lulu , Han Ruyi , Shu Qingxia , Chen Zheng-ying , Li Jinxiu
Published: June 2022

Objective

Investigation of the basic conditions and disease spectrum in neonatal intensive care unit (NICU) from 2012 to 2020, in the underdeveloped area of Xiangxi, China.

Methods

All newborns (N = 16,094) admitted to the NICU of a hospital in the Xiangxi area from 2012 to 2020 were selected for the retrospective study.

Results

The average male/female ratio was 1.43:1, with 9,482 males and 6,612 females admitted to the NICU. The sample comprised 41.02% premature infants, and 56.52% had been delivered via cesarean delivery (CD). The most prevalent diseases diagnosed in the NICU were jaundice (22.01%), respiratory (18.45%) and neurological diseases (17.54%). Over the 9-year study window, the prevalence of jaundice and cardiovascular diseases increased, while respiratory and neurological diseases became less frequent. The prevalence of the remaining diseases remained unchanged. Prevalence of neonatal diseases is influenced by gender, patient sources, delivery methods, gestational age and birth weight (P < 0.05). The prevalence of neonatal diseases was significantly higher in males, infants born via CD, and in infants of lower gestational age and birth weight.

Conclusion

The study contributes in-depth information about infant characteristics in an NICU in an undeveloped region of China. In the past 9 years, the average proportion of premature infants in the NICU decreased to 37.38% in 2020, but this figure remains higher than the Chinese national average of 26.2%. Similarly, the CD rate is higher than the Chinese average. The spectrum of neonatal diseases in the NICU in Xiangxi area is drawn, included jaundice, respiratory and neurological diseases, primarily. Through statistical analysis, it is found that the types and prevalence of neonatal diseases are closely related to different gender, gestational age, patient sources, delivery methods, and birth weight (P < 0.05). Newborns of specific gestational age, birth weight and delivery method should be considered “at-risk” and targeted in the formulation of preventive measures. There is a great need to improve the diagnosis and treatment of neonatal diseases—and perinatal health care in general—to ensure improved outcomes for newborns admitted to NICUs in underdeveloped regions.
Transseptal Approach Versus Transaortic Approach for Catheter Ablation of Left-Sided Accessory Pathways in Children
Authors: Yu Xia , Dong Ziyan , Gao Lu , Lin Li , Cui Lang , Shao Wei , Yu Wen , Zhen Zhen , Yuan Yue
Published: June 2022

Background

Catheter ablation is recommended to eradicate supraventricular tachycardia caused by left-sided accessory pathways (APs) in children. This study aims to compare the safety and efficacy of the transseptal approach (TS) and aortic approach (TA) for catheter ablation of left-sided APs in a pediatric cohort.

Methods

Patients < 18 years of age with left-sided APs who had undergone ablation at Beijing Children’s Hospital between 13 January 2018 and 7 January 2020 were included and randomly categorized into either TS or TA group (follow-up for 22 months). In all, 60 patients in TS group and 41 patients in TA group were included in this study. Principal endpoints were success rate, recurrence rate, complications, procedure time, and radiation dose.

Results

For TS group versus TA group, success rate was observed in 100 versus 97.56% (p = 0.402). The procedure time was 27.0 (32.0–23.0) versus 29.0 (38.0–24.5) min (p = 0.092). The rate of success or the procedure time was similar, but for the patients with Aps located in left posterior septum (LPS) or left posterior lateral (LPL), the TS group had a shorter procedure time compared with TA group (p < 0.01). The radiation dose was 28.0 (20.0–41.75) versus 0 mGy (p < 0.001). After successful ablation, no recurrence and complication were observed in either group.

Conclusion

Both TS and TA for catheter ablation of left-sided Aps were shown to be safe and effective in children. Zero radiation and ease of mastery make TA the preferred choice. TS is recommended to be used by properly trained medical professionals, especially for patient with AP localized in the LPL or LPS. However, TS is a good alternative where patients have aortic lesions or when TA fails.
Research Status and Hotspots of Anticancer Natural Products Based on the Patent Literature and Scientific Articles
Authors: Shen Junkai , Li Jiahuan , Yu Peiming , Du Gangjun
Published: June 2022

Background: The patent literature contains a large amount of information on the internal state of current industrial technologies that are not available in other literature studies. Scientific articles are the direct achievements of theoretical research in this field and can reveal how current theories in basic research have developed. In this study, the progress and status of natural anticancer products in this field were summarized, and the research hotspots were explored through the analysis of the relevant patent literature and scientific articles.

Methods: Patent data were retrieved from the incoPat patent retrieval database, and paper data were retrieved from the Web of Science core set and PubMed. GraphPad Prism 8, Microsoft Excel 2010, and CiteSpace 5.8.R3 were used to perform visual processing. The analyzed patent literature includes the patent applicant type, country (or region), and technical subject. The analyzed scientific article includes academic groups, subject areas, keyword clustering, and burst detection.

Results: A total of 20,435 patent families and 38,746 articles were collected by 4 January 2022. At present, antitumor drugs derived from natural products mainly include 1) apoptosis inducers such as curcumin, gallic acid, resveratrol, Theranekron D6, and gaillardin; 2) topoisomerase inhibitors such as camptothecins, scaffold-hopped flavones, podophyllotoxin, oxocrebanine, and evodiamine derivatives; 3) telomerase inhibitors such as camptothecin and isoquinoline alkaloids of Chelidonium majus, amentoflavone, and emodin; 4) microtubule inhibitors such as kolaflavanone, tanshinone IIA analog, eugenol, and millepachine; 5) immunomodulators such as fucoidan, myricetin, bergapten, and atractylenolide I; 6) tumor microenvironment regulators such as beta-escin and icaritin; 7) multidrug resistance reversal agents such as berberine, quercetin, and dihydromyricetin; and 8) antiangiogenic and antimetastatic agents such as epigallocatechin-3-gallate, lupeol, ononin, and saikosaponin A.

Conclusion: Anticancer natural product technology was introduced earlier, but the later development momentum was insufficient. In addition, scientific research activities are relatively closed, and technical exchanges need to be strengthened. Currently, the development of medicinal plants and the research on the anticancer mechanism of natural active products are still research hotspots, especially those related to immune checkpoints, essential oils, and metastatic cancer. Theories of traditional Chinese medicine (TCM), such as “restraining excessiveness to acquire harmony,” “same treatment for different diseases,” “Meridian induction theory,” and “Fuzheng Quxie,” have important guiding significance to the research of anticancer mechanisms and the development of new drugs and can provide new ideas for this process.

Systematic Review Registration: [https://sourceforge.net/projects/citespace/], identifier [000755430500001].
Comprehensive Flavor Analysis of Volatile Components During the Vase Period of Cut Lily (Lilium spp. ‘Manissa’) Flowers by HS-SPME/GC–MS Combined With E-Nose Technology
Authors: Wei Lijuan , Wei Shouhui , Hu Dongliang , Feng Li , Liu Yayu , Liu Huwei , Liao Weibiao
Published: June 2022

Volatile compounds could affect the flavor and ornamental quality of cut flowers, but the flavor change occurring during the vase period of the cut flower is unclear. To clarify the dynamic changes during the vase period of cut lily (Lilium spp. ‘Manissa’) flowers, comprehensive flavor profiles were characterized by the electronic nose (E-nose) and headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC-MS). The response value of sensor W2W was significantly higher than other sensors, and its response value reached the highest on day 4. A total of 59 volatiles were detected in cut lilies by HS-SPME/GC-MS, mainly including aldehydes, alcohols, and esters. There were 19 volatiles with odor activity values (OAVs) greater than 1. Floral and fruity aromas were stronger, followed by a pungent scent. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) could effectively discriminate lily samples derived from different vase times on the basis of E-nose and HS-SPME-GC-MS. In summary, our study investigates the flavor change profile and the diversity of volatile compounds during the vase period of cut lilies, and lilies on day 4 after harvest exhibited excellent aroma and flavor taking into consideration of the flavor intensity and diversity. This provided theoretical guidance for the assessment of scent volatiles and flavor quality during the vase period of cut lily flowers and will be helpful for the application of cut lilies during the postharvest process.
Comparative Transcriptome Analysis Reveals the Specific Activation of Defense Pathways Against Globodera pallida in Gpa2 Resistant Potato Roots
Authors: Zheng Qi , Bertran André , Brand Anouk , van Schaik Casper C. , van de Ruitenbeek Stefan J. S. , Smant Geert , Goverse Aska , Sterken Mark G.
Published: June 2022

Cyst nematodes are considered a dominant threat to yield for a wide range of major food crops. Current control strategies are mainly dependent on crop rotation and the use of resistant cultivars. Various crops exhibit single dominant resistance (R) genes that are able to activate effective host-specific resistance to certain cyst nematode species and/or populations. An example is the potato R gene Gpa2, which confers resistance against the potato cyst nematode (PCN), Globodera pallida population D383. Activation of Gpa2 results in a delayed resistance response, which is characterized by a layer of necrotic cells formed around the developing nematode feeding structure. However, knowledge about the Gpa2-induced defense pathways is still lacking. Here, we uncover the transcriptional changes and gene expression network induced upon Gpa2 activation in potato roots infected with G. pallida. To this end, in vitro-grown Gpa2-resistant potato roots were infected with the avirulent population D383 and virulent population Rookmaker. Infected root segments were harvested at 3 and 6 dpi and sent for RNA sequencing. Comparative transcriptomics revealed a total of 1,743 differentially expressed genes (DEGs) upon nematode infection, of which 559 DEGs were specifically regulated in response to D383 infection. D383-specific DEGs associated with Gpa2-mediated defense mainly relates to calcium-binding activity, salicylic acid (SA) biosynthesis, and systemic acquired resistance (SAR). These data reveal that cyst nematode resistance in potato roots depends on conserved downstream signaling pathways involved in plant immunity, which are also known to contribute to R genes-mediated resistance against other pathogens with different lifestyles.
The Impossible Stability? The Italian Lower House Parliamentary Elite After a “Decade of Crises”
Authors: Verzichelli Luca , Marino Bruno , Marangoni Francesco , Russo Federico
Published: June 2022

The article explores recent changes in the Italian parliamentary elite thanks to a novel set of data on Italian MPs between 1946 and 2018. After a first discussion on some crucial long-term trends of Italian Lower House MPs (their turnover rate, seniority, gender balance, party-related or institutional experience), we focus on the possible explanations of the profound transformation that occurred in the past decade: the rise of new party actors, the realignment between citizens and the parliamentary elites, or the use of different electoral systems. Subsequently, we point at three MP categories, taken as the most relevant proxies of the innovations in the Italian parliamentary elite. These categories are based on the length of MPs' parliamentary career, their previous party or institutional experience, and their gender. We discuss the changing numerical relevance of these categories, their parliamentary career patterns, and some features related to the institutionalization of MPs belonging to such categories. Two implications clearly emerge from our analysis. First, the changes occurred in the so-called “decade of the crises” (after the 2013 and 2018 Italian general elections) are critical in terms of a new influx of political amateur, female, and young MPs. The magnitude of this renewal can hardly be compared to any other relevant turning point of the Italian republican age and might signal the existence of a pattern of “impossible stability” for the parliamentary elite. Second, and partly in contrast with the first implication, despite such changes, the perspectives of parliamentary career and parliamentary survival remain very much subordinated to belonging to strong parliamentary party groups. This signals that, despite broad discussions about the positive role exerted by new political actors and the demand for a stronger descriptive representation, what seems to matter in the Italian Lower House is the presence of powerful political parties.
Development of Flow State Self-Regulation Skills and Coping With Musical Performance Anxiety: Design and Evaluation of an Electronically Implemented Psychological Program
Authors: Moral-Bofill Laura , López de la Llave Andrés , Pérez-Llantada Mᵃ Carmen , Holgado-Tello Francisco Pablo
Published: June 2022

Positive Psychology has turned its attention to the study of emotions in a scientific and rigorous way. Particularly, to how emotions influence people’s health, performance, or their overall life satisfaction. Within this trend, Flow theory has established a theoretical framework that helps to promote the Flow experience. Flow state, or optimal experience, is a mental state of high concentration and enjoyment that, due to its characteristics, has been considered desirable for the development of the performing activity of performing musicians. Musicians are a population prone to health problems, both psychological and physical, owing to different stressors of their training and professional activity. One of the most common problems is Musical Performance Anxiety. In this investigation, an electronic intervention program was carried out for the development of psychological self-regulation skills whose main objective was to trigger the Flow response in performing musicians and the coping mechanism for Musical Performance Anxiety. A quasi-experimental design was used with a control group in which pre- and post-measures of Flow State, Musical Performance Anxiety and, also, Social Skills were taken. Sixty-two performing musicians from different music colleges in Spain participated in the program. Results indicated that the intervention significantly improved Flow State (t = –2.41, p = 0.02, d = 0.36), and Sense of Control (t = –2.48, p = 0.02, d = 0.47), and decreased Music Performance Anxiety (t = 2.64, p = 0.01, d = 0.24), and self-consciousness (t = –3.66, p = 0.00, d = 0.70) of the participants in the EG but not CG. The changes in the EG after the program showed the inverse relationship between Flow and Anxiety. Two important theoretical factors of both variables (especially in situations of performance and public exposure), such as worry and the feeling of lack of control, could be involved. The results are under discussion and future lines of research are proposed.
Not All Daydreaming Is Equal: A Longitudinal Investigation of Social and General Daydreaming and Marital Relationship Quality
Authors: Kajimura Shogo , Nozaki Yuki , Goto Takayuki , Smallwood Jonathan
Published: June 2022

Preliminary evidence suggests that daydreaming about other people has adaptive value in daily social lives. To address this possibility, we examined whether daydreaming plays a role in maintaining close, stable relationships using a 1-year prospective longitudinal study. We found that individuals’ propensity to daydream about their marital partner is separate to general daydreaming. In contrast to general daydreaming, which was associated with lower subsequent relationship investment size (i.e., magnitude and importance of resources attached to a relationship) in the marital partner, partner-related social daydreaming led to a greater subsequent investment size. Additionally, attachment styles moderated these effects. The effect of daydreaming regarding investment size was found only in securely attached individuals. This research advances the emerging field of social daydreaming and highlights self-generated thought as a critical tool that can help people navigate the complex social world.
How Does the Change of Information Source Affect Residents’ Risk Attitudes?
Authors: Zhang Shihu , Zhang Guangcai , Li Jinpei , Gu Haiying
Published: June 2022

Using data from the China Family Panel Studies (CFPS), this paper investigates the effects of Internet use on residents’ risk attitudes. Both Generalized Ordered Logit Model and Logit model are used to identify the effects of Internet use. The results reveal an association between Internet use and increases in both subjective and objective risk preferences that remains even after we adjust for possible endogeneity. The heterogeneity analysis also reveals that these impacts are different among groups with different reasons for Internet use and different personal characteristics. Our study expands the research on the effects of Internet on people’s concepts from the micro perspective and suggests that while promoting the application of information technology we should also pay attention to the individual characteristics of residents so that we can better share the “digital dividend” brought by the popularization of information technology in the whole society.
Emotion Expression in Modern Literary Appreciation: An Emotion-Based Analysis
Authors: Li Jingxia
Published: June 2022

Background

Modern literary appreciation seems to be reading literary works phenomenally. In fact, appreciation is not a general reading, which has an important difference from general reading. It is the identification and appreciation of literary works and a complex spiritual activity for people to feel, understand, and imagine literary and artistic works. At the same time, literary appreciation is also a cognitive activity, an aesthetic activity, and a re-creation activity.

Method

In this paper, the machine learning algorithm was creatively used to classify the emotions of figures in modern literary works, to analyze the emotions of the figures that the writer wanted to depict in modern literary works.

Results

Experimental results verify the accuracy of the emotion classification method through experiments, which is helpful for us to better understand the emotion expression in modern literary works.
Investigating the Role of Perceived Information Overload on COVID-19 Fear: A Moderation Role of Fake News Related to COVID-19
Authors: Zhang Chong , Cao Tong , Ali Asad
Published: June 2022

During crises and uncertain situations such as the coronavirus disease 2019 (COVID-19) pandemic, social media plays a key function because it allows people to seek and share news, as well as personal views and ideas with each other in real time globally. Past research has highlighted the implications of social media during disease outbreaks; nevertheless, this study refers to the possible negative effects of social media usage by individuals in the developing country during the COVID-19 epidemic lockdown. Specifically, this study investigates the COVID-19 fear using the survey data collected from a developing country. In total, 880 entries were used to analyze the COVID-19 fear using the AMOS software. Findings indicated that information-seeking and sharing behavior of individuals on social media has a significant impact on perceived COVID-19 information overload. Perceived COVID-19 information overload has a positive impact on COVID-19 fear. In addition, fake news related to COVID-19 strengthens the relationship between perceived COVID-19 information overload and COVID-19 fear. The implication and limitations of the study are also discussed in the final section of the study.
The Study of the Impact of Empowering Leadership on Adaptive Performance of Faculties Based on Chain Mediating
Authors: Xu Ying , Zhang Mengliu
Published: June 2022

High-quality faculties are the fundamental guarantee to achieving the connotation development of higher education. Hence, performing university faculties determines the quality of teaching and the level of talent cultivation. Facing the change in teaching demand and environment, faculties need to change their working methods spontaneously to achieve high-level performance. Relevant empirical studies have shown that empowering leadership positively affects adaptive performance. However, some researchers have found that leadership effectiveness even has a negative effect. There may be two reasons for the inconsistency in the effectiveness of empowering leadership: (1) There is a lack of in-depth research on the effectiveness of empowering leadership and employees’ performance in existing studies, and the exploration of its theoretical mechanism should be enriched. (2) The effectiveness of empowering leadership may be subject to the conditions of the individual’s characteristics of the empowering. Therefore, the mechanism of empowering leadership on faculties’ adaptive performance still needs to be further explored. This study explores the impact of empowering leadership on adaptive performance. Based on Social Exchange Theory and Psychology Empowerment theory, this study explores the mediating role of the leadership-member exchange relationship and psychological empowerment in the relationship between them. According to Regulatory Focus Theory, the moderating role of promotion focus and prevent focus was studied. We adopted questionnaire survey data including 292 individuals in Changchun, Shijiazhuang, and other cities; STATA 15 was conducted to test the hypotheses. The results showed that: (1) Empowering leadership was significantly and positively related to adaptive performance. (2) Leader-member exchange relationship and psychological empowerment play a mediating chain role in empowering leadership and adaptive performance; empowering leadership promotes psychology empowerment by enhancing the leadership-member exchange relationship, enhancing their adaptive performance. (3) Promotion focus positively regulates the relationship between psychological empowerment and adaptive performance. Individuals with a promotion focus have a significant positive impact on adaptive performance. Individuals with preventing focus do not weaken the positive impact of psychological empowerment on adaptive performance.
Depressive Symptoms and Quality of Life in Patients With Heart Failure and an Implantable Cardioverter-Defibrillator
Authors: Zormpas Christos , Kahl Kai G. , Hohmann Stephan , Oswald Hanno , Stiel Christopher , Veltmann Christian , Bauersachs Johann , Duncker David
Published: June 2022

Background

Heart failure (HF) is associated with development of depressive symptoms and reduced quality of life (QoL). Patients with HF and an implantable cardioverter-defibrillator (ICD) were evaluated regarding depressive symptoms and QoL.

Methods

The present study included 446 patients with HF and an ICD. Depressive symptoms were assessed using the Patient Health Questionnaire 9 (PHQ-9), QoL was evaluated using the Minnesota Living with Heart Failure Questionnaire (MLHFQ). Functional ability and exercise tolerance were assessed at inclusion and after 6 months with help of the 6-min walking test (6MWT).

Results

Patients included in the study had a mean age of 65.8 years and were predominantly male (83.6%), with mostly ischemic (n = 277; 62.1%) or dilated (n = 150; 33.6%) cardiomyopathy. One hundred ninety-three (43.2%) patients had depressive symptoms, of whom 75 patients (16.8%) were classified as moderate to severe depression according to the PHQ-9 at baseline. Depressive symptoms were associated with low QoL independent of NYHA functional class. High NYHA functional class, high PHQ-9 score, age and body mass index (BMI) were associated with a lower 6MWT at enrollment, while depressive symptoms (expressed as higher PHQ-9 score) and age were associated with a lower 6MWT after 6 months. Patients with history of smoking and a higher BMI showed higher PHQ-9 scores after 6 months. Patients under antidepressant medication showed improved PHQ-9 score after 6 months, indicating controlled/treated depression. However, patients with low QoL at inclusion remained with low QoL after 6 months.

Conclusion

Depressive symptoms correlate with low QoL and lower long-term functional status in patients with HF and an ICD. Depressive symptoms are associated with smoking and obesity, which themselves are risk factors for a poor prognosis in HF. Only a small fraction of patients with HF and ICD showing depressive symptoms receives appropriate treatment. Assessing depressive symptoms and lifestyle factors should be part of a multimodal treatment plan in patients with HF and an ICD.
Multicultural Quality of Life Index in Relatives of People With Borderline Personality Disorder
Authors: Marco Jose Heliodoro , Fernandez-Felipe Isabel , García-Alandete Joaquín , Fonseca-Baeza Sara , Baños Rosa M. , Garcia-Palacios Azucena , Pérez Rodríguez Sandra , Guillén Verónica
Published: June 2022

Purpose

The aim of the present study was to confirm the original factor structure of the Multicultural Quality of Life Index (MQLI) and analyze its psychometric properties in a sample of caregivers of people with borderline personality disorder (BPD).

Methods

The MQLI was administered to 233 relatives of people with BPD. Participants completed the MQLI, the Depression, Anxiety and Stress Scale (DASS-21), and the Connor-Davidson Resilience Scale (CD-RISC).

Results

Factor analysis of the relatives indicated that the MQLI generated a one-factor solution. The MQLI showed good internal consistency, ϖ = 0.91 [95% CI (0.90, 0.93)] and correlated significantly and positively with the CD-RISC (rs = 0.576) and negatively with the DASS-21 (rs = −0.583).

Conclusion

Consistent with other studies, the MQLI demonstrated feasibility, strong internal consistency, and good convergent and discriminant validity, which means it is a psychometrically robust measure for the assessment of quality of life in relatives of people with BPD. Along with other validation studies, this measure will be a useful tool for assessing quality of life in relatives of people with mental disorders.
HIV Rapid Testing in the General Population and the Usefulness of PrEP in Ecuador: A Cost–Utility Analysis
Authors: Quirola-Amores Paulina , Espinosa Pablo , Oleas Sebastian , Hernandez Isabel , Henriquez Aquiles R. , Teran Enrique
Published: June 2022

Introduction

HIV is considered one of the most important chronic transmitted diseases worldwide. The Joint United Nations Program on HIV/AIDS in 2020 proposed the strategy “95–95–95” which goals to achieve a 95% of cases identified, receives ART, and will have achieved suppression of the virus. In Ecuador by 2020, according to the Ministry of Public Health, 45,056 persons are living with HIV, principally men between 15 and 49 years, and a mortality rate of 4.8/100,000 habitats. This study aims to determine the cost–utility of applying an early screening to a sexually active population vs. only a high-risk population and if the use of PrEP is justified depending on different contexts.

Methods

For the cost–utility evaluation, it was compared: (a) HIV screening performed only in the high-risk population vs. HIV screening in all population sexually active; and (b) the use of ART only for HIV treatment vs. ART as a treatment in diagnosed cases and the use of PrEP (only at a high-risk population of acquiring HIV). Calculation and weight of DALYs for HIV/SIDA were obtained through WHO guidelines. To generate the Markov model for HIV/AIDS, subjects were classified as symptomatic or asymptomatic, as well as the HIV deaths.

Results

Cost–benefit analysis (CUA) showed that ICER for early diagnosis had a negative value which means a saving if the strategy will be implemented as a regular test (–$591, –$4,360) and −108 and −934 DALYs, in the case of ART and PrEP, ICER the $30,541–$59,410, which resulted in more than the GDP's threshold and health years between 2,511 and 10,635 in the general population. With a reduction of 70% in the assigned budget for the early diagnosis, Ecuadorian people could lose between 4 and 6 DALYs, while if the budget reduces more than 50% to ART, it will generate a loss of 10–12 years of healthy life.

Conclusion

CUA demonstrates that an early diagnosis in a sexually active population is cost-beneficial. This, combined with ART or PrEP, is ideal to add years of healthy life.
Global Insights Into Rural Health Workers' Job Satisfaction: A Scientometric Perspective
Authors: Chen Yuquan , You Yanwei , Wang Yiche , Wang Yutong , Dai Tao
Published: June 2022

Introduction

Rural health workers (RHWs) play an irreplaceable role in ensuring and improving the health level of rural residents as the most basic and extensive medical service providers in rural areas. However, rural health institutions are facing significant worker shortages worldwide, not only in low- and middle-income countries but also in developed countries. As an important variable to explain RHWs' work status and predict turnover behavior, job satisfaction has received more and more attention currently.

Methods

Publications from 1 January 1995 to 31 December 2021 were identified from the Science Citation Index Expanded (SCI-Expanded), the Social Sciences Citation Index (SSCI), and the Emerging Sources Citation Index (ESCI) of the Web of Science Core Collection (WoSCC); CiteSpace, VOSviewer, and R software were applied to conduct this study.

Results

A total of 251 publications were obtained from the WoSCC database. The number of publications had a statistically significant increase in the study period. Ranking in the top three of the most productive countries or regions in this field was the United States, the United Kingdom, and China. “Health Care Sciences & Services,” “Nursing,” and “Public, Environmental & Occupational Health” seemed to be the major subjects. According to the reference co-citation analysis, “motivation,” “rural and remote areas,” and “work environment” were three noteworthy topics during the development of the research field. Moreover, through the keyword analysis, the underlying relationship among “job satisfaction,” “job burnout,” and “turnover intention” was explored.

Conclusion

Publications about job satisfaction associated with RHWs had remarkably indicated that this research field had great development potential and broad prospects. As an emerging topic related to RHWs' job status, job satisfaction and its related affected factors were systematically summarized by cluster and keywords analysis. We also highlighted that job satisfaction had a negative predictive effect on RHWs' job burnout and turnover intention, and job burnout played a positive role in predicting turnover intention. In addition, the job satisfaction and working environment of RHWs under the COVID-19 pandemic should receive more attention in the future.
Experimental Study on the Transport and Alteration Behavior of Aerosols From Low Density Powders for Acute Inhalation Toxicology Studies
Authors: Wessely Benno , Stintz Michael , Nolde Juergen , Creutzenberg Otto
Published: June 2022

Low density powders have a bulk density of less than 100 kg/m3 and are produced technically by flame pyrolysis of silicon tetrachloride (pyrogenic powders such as pyrogenic silica) or wet-chemically by sol-gel processes (e.g. silica-gel) or precipitation reactions using sodium silicate solution and a mineral acid. The transport and alteration behavior of aerosols from low density powders was investigated in a device for toxicological inhalation studies. The test conditions corresponded to those for acute toxicology studies according to OECD Guideline 436. The use of cascade impactors, required by guideline, has not proven successful for low density powders as the fragile agglomerate structures are destroyed during the measurement. As an alternative and non-invasive measurement method, laser diffraction spectroscopy has proved very successful in the present investigations. In particular, aerosols from pyrogenic powders of low density showed a distinctive tendency to re-agglomerate, especially at concentrations >500 mg/m3mm3. Investigation results indicate that aerosol particle size must be monitored over the entire acute inhalation test period for acute inhalation studies to be performed reliably.
Dietary Patterns in Colombia: An Exploratory and Confirmatory Factor Analysis
Authors: Meneses Urrea Luz Adriana , Vaquero Abellán Manuel , Benachi Sandoval Narly , Villegas Arenas Dolly , Osorio Murillo Olga , Molina-Recio Guillermo
Published: June 2022

This study aimed to construct and validate dietary patterns in Colombian individuals aged between 15 and 64 years. The study sample consisted of 16,216 individuals who participated in the 2015 National Survey on the Nutritional Situation of Colombia (ENSIN). To identify dietary patterns, an exploratory analysis was first conducted with 16 food groups with 70% of the study sample, followed by a confirmatory analysis with the remaining 30%. Four eating patterns were identified, referred to as traditional, industrialized, conservative, and grilled foods/drinks. The traditional pattern presented the highest variance (17.65%), followed by the industrialized (14.36%), conservative (10.74%), and grilled foods/drinks (7.57%) patterns. Regarding the frequency of food consumption, the preference for the consumption of rice/pasta and panela/sugar/honey was evident. In contrast, low consumption of whole foods, energizers, light foods, supplements, and salt was observed. The dietary patterns in Colombia continue to be characterized by a high consumption of panela/sugar/honey and low consumption of fruits/vegetables, which is why the development of public health interventions to improve these habits, such as health promotion and chronic disease prevention tools, is considered necessary.
Virtual Reality to Evaluate the Impact of Colorful Interventions and Nature Elements on Spontaneous Walking, Gaze, and Emotion
Authors: Batistatou Adamantia , Vandeville Florentin , Delevoye-Turrell Yvonne N.
Published: June 2022

Green environments are said to have a positive impact on spontaneous physical activity and well-being. However, high quality psychological measures in natural settings are difficult to collect. In the present study, we offer a detailed report on how virtual reality may provide a controlled environment for immersive user testing. Virtual Reality (VR) was here used to test the impact of colorful floor markings on the spontaneous speed of walking, gaze behaviour, as well as perceived changes in and physiological mesures of affective states. The reactions of 36 adult participants were evaluated in Grey and Green VR environments of an urban university campus. Results in VR revealed similar results than that reported in natural settings: participants walked slower and had higher heart rates in Green than in Grey urban settings, indicating more pleasurable experiences. VR results provided nevertheless more detailed description of user experience with the possibility to quantify changes in gaze strategy as a function of the presence or absence of color designs. Spontaneous walking was slower with colorful designs than without. Gaze behaviour presented longer fixation times with colorful designs than without. Finally, physiological responses indicated that mean heart rates were similar across environments and predicted the physical effort of the task. However, greater means in heart rates were observed in the environments presenting colorful designs, suggesting that colors may be a powerful tool to trigger alertness and pleasure in Grey urban cities. Virtual reality is reported here as an innovative method to quantify psychological experiences during free exploration in gait. Applicable to a broad range of research topics in the psychological sciences, explicit guidelines are made available to share computer code and data sets for further exploitation.
She'll Be ‘Right… but Are They? An Australian Perspective on Women in High Performance Sport Coaching
Authors: Roberts Alexandra H. , Clarke Anthea , Fox-Harding Caitlin , Askew Georgia , MacMahon Clare , Nimphius Sophia
Published: June 2022

Participation and media coverage of women in high-performance sport has been steadily increasing in recent years throughout the world. While this increase in interest has led to many young women and girls becoming involved in grassroots sport, there has yet to be a significant change in the number of women in coaching roles, particularly at the high-performance level. This paper synthesizes and summarizes the current challenges facing women sport coaches in Australia, drawing from existing research, media and government reports to understand the barriers for women entering and progressing in these roles. We also present some of the more recent initiatives to increase opportunities for women in high performance coaching. Within Australia, there is a need to (1) understand the pipeline for women coaches, (2) examine the interacting contexts and constraints that women are subject to within sporting organizations, and (3) create a preliminary framework for future research, outreach, and education to address gender inequity within Australian sport coaching.
Talent Identification and Development in Paralympic Contexts: Current Challenges
Authors: Dehghansai Nima , Pinder Ross A. , Baker Joe
Published: June 2022

This short review explores the state of talent identification and development of athletes in Paralympic contexts. While talent identification typically occurs during adolescence, this practice is more complex and variable in Paralympic contexts compared to non-Paralympic contexts. For example, Paralympic athletes can have impairments that are congenital or acquired at any time across their lives. Therefore, they can enter performance pathways at unpredictable times. Furthermore, differences and nuances associated with athlete impairments (type and severity), compounded by other systematic complexities (e.g., systems of classification) highlight the need to consider alternative and creative approaches to talent identification and development. We provide an overview of some of these complexities, highlight areas for future research, and provide recommendations for practitioners.
A New Immortalized Human Alveolar Epithelial Cell Model to Study Lung Injury and Toxicity on a Breathing Lung-On-Chip System
Authors: Sengupta Arunima , Roldan Nuria , Kiener Mirjam , Froment Laurène , Raggi Giulia , Imler Theo , de Maddalena Lea , Rapet Aude , May Tobias , Carius Patrick , Schneider-Daum Nicole , Lehr Claus-Michael , Kruithof-de Julio Marianna , Geiser Thomas , Marti Thomas Michael , Stucki Janick D. , Hobi Nina , Guenat Olivier T.
Published: June 2022

The evaluation of inhalation toxicity, drug safety and efficacy assessment, as well as the investigation of complex disease pathomechanisms, are increasingly relying on in vitro lung models. This is due to the progressive shift towards human-based systems for more predictive and translational research. While several cellular models are currently available for the upper airways, modelling the distal alveolar region poses several constraints that make the standardization of reliable alveolar in vitro models relatively difficult. In this work, we present a new and reproducible alveolar in vitro model, that combines a human derived immortalized alveolar epithelial cell line (AXiAEC) and organ-on-chip technology mimicking the lung alveolar biophysical environment (AXlung-on-chip). The latter mimics key features of the in vivo alveolar milieu: breathing-like 3D cyclic stretch (10% linear strain, 0.2 Hz frequency) and an ultrathin, porous and elastic membrane. AXiAECs cultured on-chip were characterized for their alveolar epithelial cell markers by gene and protein expression. Cell barrier properties were examined by TER (Transbarrier Electrical Resistance) measurement and tight junction formation. To establish a physiological model for the distal lung, AXiAECs were cultured for long-term at air-liquid interface (ALI) on-chip. To this end, different stages of alveolar damage including inflammation (via exposure to bacterial lipopolysaccharide) and the response to a profibrotic mediator (via exposure to Transforming growth factor β1) were analyzed. In addition, the expression of relevant host cell factors involved in SARS-CoV-2 infection was investigated to evaluate its potential application for COVID-19 studies. This study shows that AXiAECs cultured on the AXlung-on-chip exhibit an enhanced in vivo-like alveolar character which is reflected into: 1) Alveolar type 1 (AT1) and 2 (AT2) cell specific phenotypes, 2) tight barrier formation (with TER above 1,000 Ω cm2) and 3) reproducible long-term preservation of alveolar characteristics in nearly physiological conditions (co-culture, breathing, ALI). To the best of our knowledge, this is the first time that a primary derived alveolar epithelial cell line on-chip representing both AT1 and AT2 characteristics is reported. This distal lung model thereby represents a valuable in vitro tool to study inhalation toxicity, test safety and efficacy of drug compounds and characterization of xenobiotics.