bluuebunny/crossref_metadata_2025 huggingface dataset:
HPTLC Densitometric Analysis for Quantification of Sennocide-B and Rhein in Leaf Extracts of Senna alexandrina Mill. and Their Commercial Formulations.
Authors: Ahmad Rizwan . , Fatima Zeeshan . , Ali Sadath . , Dhaneshwar Suneela .
Published: October 2024

The leaf extract of Senna alexandrina Mill., a plant from the Fabaceae family, contains bioactive anthraquinones sennoside-B and rhein, traditionally used in Indian medicine for various health issues. However, standardizing the quality of S. alexandrina and its commercial products is challenging. To address this, we established two high-performance thin-layer chromatography (HPTLC) methods for accurate analysis of these biomarkers in S. alexandrina leaf extract and its commercial formulations. For sennoside-B, a mobile phase combination of solvent for example, butanol, water, and glacial acetic acid (6:3.5:0.5, v/v/v) was used, with detection at 254 nm, producing spots at an Rf value of 0.37 ± 0.006. The method showed linearity between 100 to 2000 ng/spot (R² = 0.9983 ± 0.0017), with a limit of detection (LoD) of 22.84 ± 0.554 ng/band and a limit of quantitation (LoQ) of 69.22 ± 0.859 ng/band. For rhein, a mobile phase combination of solvent toluene, ethyl acetate, and glacial acetic acid in the ratio (7:2.5:0.5, v/v/v) was utilized and was also detected at 254 nm. The obtained spots had Rf value of 0.67 ± 0.004. The linearity was confirmed within the same range (R² = 0.9985 ± 0.0005), with an LoD of 15.49 ± 0.645 ng/spot and an LoQ of 46.94 ± 1.172 ng/spot. Herein, the methods have followed the guidelines of ICH (Q2) R1. These developed HPTLC methods are selective, simple, sensitive, accurate, and economical, making them suitable for everyday assessment of these bioactive markers in S. alexandrina and its commercial products. This study supports the quality control of S. alexandrina, ensuring product consistency, efficacy, and safety.
Predictors of Pre-Hypertension and Hypertension in Rural Adults: Insights Into the Impact of Yoga, Lifestyle, and Demographic Factors
Authors: Madasamy Senthil , Arora Namita , Arora Pankaj
Published: October 2024

Background: Hypertension is a leading risk factor for cardiovascular diseases, particularly in rural areas where healthcare
access is limited. This study investigates predictors of pre-hypertension and hypertension, with a focus on yoga and lifestyle
factors such as physical activity, smoking, and alcohol consumption.
Methods: A cross-sectional study was conducted among rural adults to collect data on demographic factors (age, gender,
education level), lifestyle habits (smoking, alcohol, physical activity), and yoga participation. Blood pressure was measured
and classified into normal, pre-hypertension, and hypertension categories. Logistic regression was used to identify
significant predictors of hypertension and pre-hypertension.
Results: Key predictors of hypertension include age (OR = 0.998), gender (OR = 0.648 for males), and BMI (OR = 1.012).
Alcohol consumption was significantly associated with higher hypertension risk (OR = 1.293). Yoga participation,
particularly improvements in flexibility, showed a modest reduction in hypertension odds (OR = 0.945). Non-yoga physical
activity also demonstrated a protective effect (OR = 0.958).
Conclusion: Lifestyle factors such as alcohol consumption and physical activity play important roles in hypertension risk.
While yoga showed some benefits, particularly in flexibility improvements, further research is needed to explore its longterm
effects. Public health interventions targeting modifiable risk factors, including yoga, may help reduce the prevalence
of hypertension in rural communities.
Isolation, Characterization and Determination of Structural Composition of Ocimum Tenuiflorum (Krishna Tulsi Plant) by IR, NMR, Mass Spectroscopy.
Authors: Singh Jay Prakash , Tripathy Subhashish , Srivastava Saloni , Chaurasia Veerendra Kumar
Published: October 2024

therapeutic and medicinal uses. This evergreen leaf, which is indigenous to the Indian subcontinent, is well known
around the world for its versatility and capacity to treat a wide range of illnesses. Objective: The primary goal of this
bioanalytical investigation is to identify and structurally clarify the principal phytoconstituents found in the Ocimum
tenuiflorum ethanolic extract. Method: In this phyto-analysis, spectral analysis was used to clarify the structure of the
phytoconstituents that were extracted from the Ocimum tenuiflorum plant using column chromatography.
Result and Conclusion: Using spectrum analysis (I.R., NMR, and mass spectroscopy), two significant isolates from
Ocimum tenuiflorum, Apigenin and Carnosic Acid, were found and their structures clarified.
Simultaneous Estimation of Monoterpenes (Eugenol, Eucalyptol and R-Limonene) by HPTLC Densitometric Method and Its Validation.
Authors: P Singh . , SK Yadav . , MK Gupta . , S Sardana .
Published: October 2024

Plant-derived monoterpenes have various pharmacological applications, but the problem lies with their detection and estimation. Hence, it is crucial to develop a robust technique to overcome this problem. This work was aimed at developing a swift, accurate and reproducible technique for the simultaneous detection of three monoterpenes, namely, eugenol, eucalyptol (1,8- cineole), and R-limonene, using the high-performance thin-layer chromatography technique, as per International Council of Harmonization (ICH) guidelines. The best results were obtained in the eluent mixture prepared by using hexane: toluene: ethyl acetate (6:3:1, v/v/v), and silica gel-coated aluminum thin layer chromatography (TLC) plates 60F254 as a stationary phase that produced very sharp and well-resolved symmetrical peaks for eugenol, eucalyptol, and R-limonene at Rf values of 0.47 ± 0.03, 0.56 ± 0.03, and 0.71 ± 0.03, respectively. The linear range for eugenol, eucalyptol, and R-limonene was 1 to 15 ng/ spot (r2 = 0.9906), 100 to 600 ng/spot (r2 = 0.99625), 50 to 300 ng/spot (r2 = 0.9873), respectively. Furthermore, the limit of detection (LoD) and limit of quantitation (LoQ) values for eugenol were obtained at 0.0129 and 0.0391 ng/spot, followed by eucalyptol (0.82 and 2.48 ng/spot) and R-limonene (0.594 and 1.8 ng/spot), respectively. To our knowledge, we are the first to report a quick and reproducible high-performance thin-layer chromatography (HPTLC) technique for the simultaneous determination of eugenol, eucalyptol (1,8-cineole), and R-limonene. This method can further be used for the quantification of these secondary metabolites in plant extracts, enriched fractions, and pharmaceutical formulations.
Synthesis and Biological Evaluation of Novel Imidazolone - Thiabendazole-Based Metal Complexes.
Authors: SR Chaudhari . , PN Patil . , YS Parab . , NS Pawar .
Published: October 2024

Thiabendazole-based metal complexes are attracting interest due to their special antimicrobial activities. The current investigation describes the preparation of ligand L1 and its metal complexes [M (C26H17N5OS) Cl2] M = Co, Ni, Cu and Zn. Mass, 1 H-NMR, IR, and UV were used to characterize these compounds. L1 exhibited the highest potency towards many bacteria. It shows the maximum zone of inhibition as compared to any other ligand. Thus, the antibacterial potency of L1 is better as compared to standard drugs. Imidazolone-TBZ ligands L1 are also more susceptible to some bacteria and also show antifungal potency against Pseudomonas aeruginosa. The significance of such work triggered the possibility that these ligands L1 and metal complexes can be efficacious drugs against bacterial and fungal species that could be advantageous in designing powerful antibacterial and antifungal compounds for medicinal use. All these synthesized compounds are in addition to the library of heterocyclic compounds.
Phytochemical Profiling of Pleurotus Pulmonarius from Paddy Straw Substrate.
Authors: S Aswathy . , J Manjunathan .
Published: October 2024

This paper focuses on the cultivation of Pleurotus pulmonarius mushroom, to find the Preliminary phytochemical analysis from five different solvent extracts of P. pulmonarius. Mushrooms are white fungi that are recognized as a good food source and contain a variety of medicinal benefits. In terms of nutrition, mushrooms are better than a lot of veggies because it contain around 40 to 49% protein. The current study integrates phytochemical analysis with the production of P. pulmonarius mushroom. The mushroom grew on paddy straw, which had been used as a substrate. The Pleurotus mushroom accounts for 25% of the total edible mushroom production worldwide. Fresh mushroom fruit bodies were picked, dried, pulverized, and extracted using a soxhlet apparatus. The pH, humidity and temperature of the surroundings were maintained in between the mycelia growth. Preliminary phytochemical analysis of five different extracts from P. pulmonarius was tested using various solvents, such as alkaloids, flavonoids, terpenoids, tannins, steroids, glycosides, saponins, proteins, quinones, carbohydrates and phenols. Further research can be conducted to determine the bioactive substances from this particular mushroom.
Polyherbal Formulation Development, Evaluation and Pharmacological Screening for Hepatoprotective Activity.
Authors: SS Hindole . , SM Vijayendraswamy . , RS Sakhare .
Published: October 2024

The current research work investigates the hepatoprotective action of polyherbal formulation prepared from ethanolic extracts of Azadirachta indica A. Juss, Moringa oleifera Lam, Cymbopogon citrates (DC.) Stapf, Tinospora cordifolia (Thumb.) Miers, Ricinus communis L., against experimental hepatotoxicity. The World Health Organization (WHO) is promoting herbal remedies derived from traditional medicinal plants in several countries. The main goal of this investigation was to create and evaluate the polyherbal formulation to protect albino Wistar rats against the hepatotoxic effects of CCl4. Three potential suspensions, polyherbal formulations A, B, and C, were formulated by trituration method using ethanolic extracts of specific medicinal plant components with different sodium carboxy methyl cellulose concentrations 1, 1.5, and 2%, respectively and evaluated the same for both certain physiochemical parameters, including organoleptic characteristics, pH, viscosity, sedimentation volume, redispersibility, density, specific gravity, zeta potential, and accelerated stability studies for three months and for hepatoprotective activity also. Evaluation parameters for Polyherbal Formulation-C have shown good results that met the specifications, with pleasant texture and appearance. Along with other physiochemical qualities, like pH, viscosity, flow rate and sedimentation, were all unchanged and showed significant hepatoprotective effects by reducing elevated serum enzyme levels SGOT, SGPT, ALP, total and direct bilirubin. It was discovered that each quality control parameter in the polyherbal suspension C formulation was thoroughly adequate and improved the recovery from hepatotoxicity caused by CCl4. These findings suggest that the produced polyherbal suspension C (containing ethanolic extracts of A. indica A. Juss, M. oleifera Lam, C. citrates (DC.) Stapf, T. cordifolia (Thumb.) Miers, R. communis L. may be stable, secure, and effective for use and exhibited notable hepatoprotective effects, possibly resulting from the combined impact of all these extracts.
Evaluating the Efficacy of Entonox in Reducing Labor Pain During Active Labor: A Case-Control Study.
Authors: Khan Pathan Amanulla , Samreen Ayesha . , Sadaf Sofia . , Latha D Naga , Varun D .
Published: October 2024

The experienced of pain amid labor is women’s most extreme event, which causes intemperate stress. Assorted in strength, every woman bears intensity of labor is varied. Entonox is one of the non-invasive analgesic methods used during the process and identifying its potency is the foremost article’s purpose. A hospital-based prospective case control-single-centered study was done. The subject’s information and feedback on entonox use were gathered and analyzed with suitable statistical SPSS. The explicit parameters of parturient were interpreted by descriptive frequencies comparison between the different variables were calculated using the T-test. Many entonox users showed positive responses with reduced pain compared to non-users. The Apgar score showed no significant variation in both I and II groups, which proves no alliance of entonox with neonatal outcomes. Entonox is a choice alternative for managing pain during the initial labor phases. The success of this preferable technique (Entonox) can be attributed to most females choosing non-intrusive methods during labor.
An In-Depth Analysis of the Influence of Pharmaceutical Marketing Strategies on the Prescription Practices and Decision-Making Processes of Medical Professionals.
Authors: Singh Monu . , . Pallavi . , R D. . , Manickam Ramachandran .
Published: October 2024

The study seeks to explore how pharmaceutical marketing strategies impact doctors’ prescribing habits within the complex relationships between healthcare providers and pharmaceutical companies. Five alternative pharmaceutical products are analyzed across six distinct evaluation parameters to understand the multifaceted impact of marketing efforts. The dataset includes information on marketing spend, prescription rates, and various evaluation criteria such as efficacy, safety, affordability, brand recognition, promotional materials, reputation, and convenience. These parameters are crucial in assessing the comprehensive influence of pharmaceutical marketing on doctors’ decision-making processes. Understanding how pharmaceutical marketing impacts prescription practices is crucial for ensuring patient welfare and safety. It helps in evaluating whether prescriptions are driven by genuine medical needs or influenced by marketing strategies, thus promoting patient-centered care. The study addresses ethical concerns related to the interactions between pharmaceutical companies and healthcare professionals. Uncovering the extent of marketing influence aids in developing ethical guidelines and regulations, fostering transparency and trust in the healthcare system. The COPRAS-G method necessitates identifying selection criteria, evaluating relevant information for these criteria, and creating methods to assess how well the surrogate meets the participants’ needs. Decision analysis involves a Decision Maker (DM) who must consider a specific set of alternatives and choose one from several options, often with conflicting criteria. Consequently, the developed complexity proportionality assessment (COPRAS) method can be applied. The results indicate that Doctor 03 ranked first, while Doctor 05 ranked the lowest.
UPLC Estimation of an Antidiabetic Drug by Method Development and Validation Followed by Stability Indicating Studies.
Authors: Swarnalatha S. . , Moorthy Revathy Sundara , Rondla Rohini . , Pingili Kavitha . , Reddy P. Muralidhar
Published: October 2024

The analytical method development and validation of a common antidiabetic drug, Canaglifozin, by taking the aid of ‘Ultra High-Performance Liquid Chromatography’, along with stability indicating method. The canaglifozin drug was eluted from the optimized mobile phase in 70:30 ratio of water and methanol, at 1 ml/ min flow rate, under separation with C18 column of 100 x 2mm size with 1.8 diameters at isocratic mode. The detection was carried at 286 nm wavelength, resulting in 3.80 retention time, as run time being 10 min. The detection limit and quantification limit, was noticed at ‘0.0039 and 0.0119 µg/ ml’ correspondingly. The percentage RSD and recovery percentage was under ICH guidelines only. The stress degradation studies under acid, basic, neutral, and thermal conditions were carried on.
Preparation and Characterization of Solid Dispersion of Sorafenib
Tosylate for Enhancement of Bioavailability.
Authors: Chivate Anuradha Niranjan , Sakhare Pritam Mohanlal , Chivate Niranjan Dattatray , Bhosale Rohit Rajendra , Gandhi Jotsna Mohanlal
Published: October 2024

Solid dispersions have garnered significant attention as an effective strategy for enhancing the solubility and, consequently, the bioavailability of poorly water-soluble drugs. By forming solid dispersions of these drugs with water-soluble carriers, issues related to limited solubility have been mitigated, leading to improved dissolution rates. The core focus of solid dispersion technology revolves around the creation of two-component systems involving the drug and a polymer, where the successful dispersion and stabilization of the drug play pivotal roles in the formulation development process. As a result, this technology is widely acknowledged as a highly valuable approach for enhancing the dissolution properties of poorly water-soluble drugs. In recent years, a substantial body of knowledge has been amassed concerning solid dispersions. Nonetheless, their practical implementation in the commercial sphere remains somewhat limited. In the current investigation, we embarked on the preparation of a solid dispersion of sorafenib tosylate, with the primary objective of achieving the maximum release of the drug within the shortest possible timeframe. Our aim was to develop and optimize the solid dispersions of sorafenib tosylate in a manner that ensures the successful design and formulation of this system.
Development and Evaluation of Flurbiprofen-Ferulic Acid Co-crystal With Enhanced Solubility and Dissolution Rate.
Authors: Nalawade Akshay . , Yadav Shubham . , Sankpal Poournima . , Tamboli Amir .
Published: October 2024

Flurbiprofen, a BCS class II NSAID, was combined with GRAS molecules to create new co-crystal forms using crystal engineering techniques such as solvent drop grinding and evaporation. Analysis with XRD, DSC, and FTIR confirmed purity and co-crystal formation. X-ray crystal data revealed hydrogen bonding and interactions, while DSC showed changes in thermal behavior. The co-crystals, particularly the flurbiprofen-ferulic acid co-crystals, had increased solubility and faster dissolution than pure drug.
Analysis of the Relative Effectiveness of Curcumin Loaded Chitosan Nanoparticles and Pure Curcumin Compound in the Treatment of Leishmaniasis.
Authors: Mude Gaurav . , Manekar Snehal . , Polakhre Gayatri . , Yawalkar Pratiksha .
Published: October 2024

Aim: A proficient drug conveyance plan is expected to treat cutaneous leishmaniasis (CL). Regardless of their deficient solvency, significant expense, low assimilation, and rising medication-safe Leishmania spp., pentavalent antimonials and curcumin (CC) may fix leishmaniasis. Drug conveyance frameworks (DDS) might be utilized to treat CL without these worries. Methodology: We tried free CC and Curcumin-stacked nanoparticles produced using chitosan (CC-CNPs) for antileishmanial movement in-vitro. Nanoparticles of Chitosan (CNPs) were produced by tripolyphosphate (TPP), which are negatively charged ionic gelation. CC was added to the CNPs mix. The size, surface shape, epitome adequacy (EE), drug stacking content (DLC), and surface charge of the NPs were characterized using various approaches. The MTT test was performed to evaluate their effectiveness against Leishmania tropica promastigotes and axenic amastigotes after characterization. Results: Round CC-CNPs with a typical molecule size of 276 nm, a zeta capability of (+18.74 mV), and 88% embodiment viability were integrated. Following 72 hours, free CC restrained promastigotes and axenic amastigotes parasite load by 64 and 70%, separately, though CC-CNPs repressed parasites by 91 and 86%. CC-CNPs diminished parasite suitability better than free CC. The half-maximal inhibitory focus (IC50) of CC-CNPs was a lot of lower than that of free CC. Conclusion: In a portion-time subordinate way, CC-CNPs showed more antileishmanial action than unbound CC. In-vivo testing is expected for CL nearby treatment with this detailing.
Development and Validation of Stability Indicating HPLC Method for the
Determination of Related Substances in Droxidopa Drug Substance.
Authors: Gannamani Kiran Kumar , Chintakula Sudhakar .
Published: October 2024

Droxidopa, a synthetic amino acid precursor to norepinephrine, was licensed by the FDA in 2014 to treat neurogenic OH. The enzyme L-amino acid decarboxylase in sympathetic neurons converts Droxidopa to norepinephrine, which raises the amount of norepinephrine in the blood.1 Because noradrenaline crosses the blood-brain barrier poorly. Droxidopa is used to raise the concentration of noradrenaline in the brain instead of noradrenaline itself. It is recommended for the treatment of dopamine beta-hydroxylase deficiency, non-diabetic autonomic neuropathy, orthostatic dizziness, and lightheadedness in adult patients with symptomatic neurogenic orthostatic hypotension resulting from primary autonomic failure (Parkinson’s disease, multiple system atrophy, and pure autonomic failure.2-5 It is a synthetic analog of catecholamine acid that raises norepinephrine and epinephrine levels throughout the body by being immediately metabolized by dopa-decarboxylase. In the CNS, it also penetrates the blood-brain barrier to carry out its pharmacological actions. Through the induction of peripheral arterial and venous vasoconstriction, it raises blood pressure peripherally and causes slight, momentary increases in plasma norepinephrin.
Implementing Stacking with Cross - Validation for Heart Disease Prediction.
Authors: Prajapati Yogendra Narayan , Balon Dev .
Published: October 2024

The objective of this work is to apply machine learning techniques for the prediction and early identification of cardiovascular disease, a major worldwide health problem. XGBoost, K-nearest neighbors (KNN), decision tree (DT), support vector machine (SVM), and StackingCVClassifier were among the ensemble algorithms used to anticipate cardiac disease using a dataset that came from the UCI ML database. To improve the quality of the data, an exploratory data analysis was performed on the dataset using methods including outlier identification and missing value imputation. Stacking CV Classifier attained the best accuracy rate of 92.20%, according to a comparative examination of pre-processing and post-processing findings. When compared to previous approaches, the suggested strategies performed better in terms of accuracy, recall, and f1-score. Moreover, the flexibility of the model indicates its possible application to other illnesses with comparable features.
Assessment of intranasal in-situ gel efficacy of ethanolic extract of Mimosa pudica for anxiolytic and antidepressant activity: Formulation optimization and stability studies.
Authors: Deka Kamal . , Bhattacharjee Atanu . , Debnath Subhashis . , Chakraborty Raja .
Published: October 2024

This research delves into the therapeutic potential of Mimosa pudica, known as chuimui or lajwanti in Hindi, for alleviating anxiety and depression, capitalizing on its diverse pharmacological activities. Renowned for analgesic, antidiarrheal, antiinflammatory, hepatoprotective, antiasthmatic, anti-ulcer, and antioxidant properties, the plant emerges as a promising herbal remedy for mental health. The study specifically accentuates its antidepressant and anxiolytic properties, aiming for minimal side effects compared to synthetic alternatives. Pre-formulation considerations involve optimizing the concentration of polymer for in-situ gelation. Preliminary phytochemical screening unveils the presence of bioactive compounds, including terpenoids, flavonoids, glycosides, alkaloids, quinines, phenols, tannins, and saponins, known for modulating neurotransmission and exhibiting anxiolytic effects. The concentration of deacetylated gellan gum for gelation is fine-tuned using simulated nasal fluid, emphasizing minimal viscosity. Compatibility studies using Fourier-transform infrared spectroscopy (FTIR) confirm the absence of significant drug-polymer interactions. Differential scanning calorimetry (DSC) provides insights into thermal behavior. Six trial batches with varying concentrations are prepared, and final formulations are evaluated for different physicochemical parameters. Stability studies are conducted under different conditions for three months. The comprehensive investigation aims to inspire advanced research into the manifold benefits of Mimosa pudica, particularly in mental health treatment, paving the way for potential herbal formulations with reduced side effects.
Formulation and Evaluation of Floating Tablets Using Natural Polymer.
Authors: Kumar Sahil . , Rana Ritesh . , Devi Priyanka . , Sharma Puneet .
Published: October 2024

Gastro-retentive effervescent floating tablets containing quetiapine fumarate (QP) are available using different viscosity grades of HPMC (HPMC K4M, HPMC K15M, and HPMC K100M) and TD made by the direct compression technique.The outcomes of the pre-compression parameters indicate that the powder mix of the formulations of both drugs has shown good micromeritic properties. Every tablet formulation that was made had the same amount of drug in it. The low standard deviation results imply that the drugs are distributed uniformly throughout the matrices.Drug compatibility with excipients is demonstrated by DSC and FTIR testing. It was discovered that the floating lag time was influenced by the concentration of citric acid and sodium bicarbonate. All the formulations (QP1 to QP17) floated for more than 24 hours, while the QP formulations remained buoyant for more than 12 hours. Accordingly, the present study’s results suggest that the QP floating system has a promising future as a substitute for the corresponding conventional dosage form.
QbD-Driven Approach to Cleaning Method Development and Validation for Darunavir Analysis in Oral Films.
Authors: T Anusha . , R Prasanthi . , Tirunagari Mamatha . , Buggana Siva Jyothi , Mitta Chaitanya . , Remidicherla Swetha Sri
Published: October 2024

The current investigations involve developing a high-performance liquid chromatographic method that’s simple to operate, fast to establish, accurate, precise, and economical through design-enabled methods. A positive experimental design is offered to utilize the central composite design of pH and the mobile phase, two crucial components of the RP-HPLC technology. The chromatographic conditions were optimized with the release of Design Expert software version 13.0. Symmetry C18 column (4.6×150mm, 5µm) was used in the procedure, along with acetonitrile (20:80 v/v) and potassium dihydrogen orthophosphate buffer pH 4.0 as the mobile phase. There was a 0.70 ml/min flow rate. 263 nm is the wavelength of detection. Darunavir’s sharp, resolved peak was seen 2.3 minutes after swabbed samples from the 10*10 cm SS plate were injected. A linear calibration curve with an R2 of 0.9991 was observed in the concentration range of 0.25 to 25µg/ml. For precision %RSD is 1.03,1.48. Accuracy % concentration 50%,100%,150% mean recovery 99.43-100.53.LOD & LOQ 1.12µg/ml, 3.39µg/ml. Robustness was tested with modest modifications to the wavelength and flow rate. Forced degradation studies were carried out and found to be within the limits. the degradation parameters such as Acid, Base, Oxidative, Photolytic, and thermal degradation parameters are according to the ICH guidelines. % Assay was done for oral films and it was found to be within the limits. Thus, the outcomes unequivocally demonstrated that the QbD technique could be effectively used to enhance the HPLC method for the measurement of darunavir in production settings.
Development and Validation of a Stability Indicating RP-HPLC Method for Simultaneous Estimation of Metformin, Tenegliptin, and Pioglitazone in bulk and its Pharmaceutical Dosage Form .
Authors: Bindu M . , Gandla Kumaraswamy .
Published: October 2024

A straightforward, reliable, and thorough approach was created for contemporaneously estimating Teneligliptin, Metformin, and Pioglitazone in solid dosage form. It was conducted by means of Agilent C18 150x4.6mm, 5µ. The mobile phase comprising buffer, acetonitrile, and Methanol (55:35:10 v/v) passed across the column at a pace of 0.9ml/min. The procedure relied on a 0.01N K2HPO4 buffer having a pH of 3.5, which was achieved by diluting an orthophosphoric acid solution. The temperature was kept constant at 30°C—optimized wavelength for Teneligliptin. Metformin and Pioglitazone were 220.0 nm. Retention times of Teneligliptin, Metformin, and Pioglitazone were 2.970 min, 2.395 min, and 3.642 min. %RSD of a system for Teneligliptin, Metformin, and Pioglitazone were 1.3, 0.6, and 1.0, respectively. %RSD of a method for Teneligliptin, Metformin, and Pioglitazone was 0.6 for each drug. The % recovery was 100.43, 100.45, and 100.22% for Teneligliptin, Metformin, and Pioglitazone, accordingly. The regression equations are used to generate the LoD and LoQ values of Teneligliptin, Metformin, and Pioglitazone, and LoD values were 0.016 ppm, 0.26 ppm, 0.42ppm, while LOQ values of Teneligliptin, Metformin and Pioglitazone were 0.048 ppm, 0.78 ppm,1.28 ppm respectively. The regression equation of Teneligliptin was y = 56655x + 920.87. Metformin was y = 14194x + 3082.1 and of Pioglitazone was y = = 41330x + 792.61.Reduced retention durations make the new approach easy and cost-effective for use in routine industrial quality control testing.
PDA Therapy in Preterm Infants: Early Treatment (NSAID, acetaminophen or combination) versus Watchful Waiting.
Authors: Roushdy Alaa Mahmoud , AbdelRahman Tarek M. , Mohamad Mahmoud Abozaid , Gambire Makarand Suresh , Farrag Hazem M. A.
Published: October 2024

Drug therapy targeted for PDA closure is one of the most controversial topics, especially among preterm infants. NSAIDs have been the most widely prescribed drugs for this purpose. The argument included early drug treatment versus watchful waiting (i.e., non-pharmacological strategy) and single versus combination therapy. Previous studies concluded that early use of NSAIDs like ibuprofen resulted in a high rate of nonsurgical closure of PDAs in most treated preterm infants. Recently, the impact of early use of ibuprofen among preterm infants was studied in 2 trials. One concluded that early ibuprofen administration wasn’t related to a lower risk of death or severe bronchopulmonary dysplasia than a placebo. The other one concluded that early PDA therapy was associated with lower rates of deaths, and this effect was pronounced among groups who required invasive ventilation. Considering that acetaminophen has fewer adverse effects, a novel strategy using combination therapy (NSAID + acetaminophen) had emerged - for pharmacological PDA closure in preterm infants - as an alternative to NSAID alone with similar efficacy and fewer side effects.
Synthesis of thiophene hydrazone derivatives by Japp Klinegmann reaction method for anticancer activity against MCF-7 cell line.
Authors: V Chopade V , S Gawade V , V Puri M , C Hol K , G Chaudhari R
Published: October 2024

The creation of novel anticancer drugs is the aim of this research. Our decision about which compounds to synthesis was aided by docking studies. Using data from the protein databank and refinement from BIOVIA discovery, the protein structure of AGF183 (PDB5IZQ). It was subjected to molecular docking investigations using PyRx 0.8 Autodock Vina software. following the creation of a novel sequence of thiophene-2,5-carbohydrazide. Final chemicals such as thiophene-2,5-carbohydrazide derivatives (G1 and G2) are produced via the Japp Klinegmann process. This reaction makes it possible to produce Schiff bases without any problems. Diethylthiophene-2,5-dicarboxylate was reacted with an aromatic diazonium salt to create the final products. When compared to the reference drug imatinib, the synthesized compounds exhibited the strongest anticancer effect. The study conducted on MCF-7 cell line demonstrated that compounds G1 and G2 demonstrated the most potent anticancer effect. Compared to the reference value of imatinib, which is 52.77 µg/ml and has G1 and G2 docking scores of -8.8, -8.6, and the standard medication methotrexate (-11.87 kcal/mol), their IC50 values were 46.52 µg/ml and 50 µg/ml. On base of characterization results above show that it Japp Klinegmann Synthesis method is useful for synthesis of thiophene hydrazone derivatives and its findings imply that the MCF-7 cells, which are utilized to potential treatment for human breast cancer.
Pharmacokinetic and Pharmacodynamics Formulation Aspects of β-Sitosterol.
Authors: TP Durgawale . , AC Rahate . , PP Nikam . , LS Nemade LS .
Published: October 2024

Sterols have a crucial role in the structure of cells of plants and animals membranes. Animals and plants are rich in cholesterol and beta-sitosterol, respectively. Vegetarians consume a lot of β-sitosterol, but their absorption is quite low. Because of their structural similarities, beta-sitosterol competes with cholesterol for absorption and is consequently utilized as an antihyperlipidemic drug. ß-sitosterol’s pharmacological screening showed a variety of non-toxic antibacterial, anti-inflammatory, antitumor, antifertility, angiogenic, antioxidant, immunomodulatory, and antinociceptive properties. It is also well-studied how β-sitosterol pharmacokinetics work. Numerous authors have produced several formulas. Nevertheless, dearth of research reported on the pharmacology, phytochemistry, and market outlook of β-sitosterol.
Perineal fistulation secondary to retained appendicolith: A rare complication of the unaccounted calculus
Authors: Charters Pia F. P. , Lowe Andrew Stephen , Burn Paul , Vickery Christopher
Published: October 2024

An approximately 30-year-old male patient presented with perianal fistula disease. Examination under anesthetic revealed a complex extrasphinteric tract with internal opening in the region of the anterior inferior rectum, although an internal opening was not clearly identified. This failed to heal despite defunctioning procedure and packing of the perineal tract. Specialist center review of computed tomography and magnetic resonance imaging revealed a small cavity anterior to the rectum containing a small calcific density with a long tract extending down into the perineum. Seven years earlier, the patient had presented with clinical features of appendicitis. On retrospective review, an appendicolith can be seen on ultrasound and abdominal X ray (AXR). At surgery, the appendix had disintegrated leaving an abscess cavity. The appendicolith was not appreciated or removed and subsequently migrated into the pelvis acting as a source of infection which fistulated to the perineum. Subsequent removal of the calculus resulted in complete healing.
Balancing risks and outcomes in schwannoma treatment: Case series
Authors: Saket Kumar , Mahableshwara C. H. , Kumar D. Prasanna , Sutradhar Dibakar
Published: October 2024

Schwannomas are rare benign tumors originating from Schwann cells of peripheral nerve sheaths, occasionally presenting intraosseously in the oral and maxillofacial region. Management decisions for these tumors require careful consideration of their location, size, and impact on surrounding structures. We present two contrasting cases of schwannomas: One involving a non-surgical approach due to extensive involvement and proximity to critical structures and the other necessitating surgical excision for a well-defined intraosseous lesion. The first case, in a 28-year-old female, highlighted the decision to monitor the lesion due to its significant size and location, while the second case, in a 20-year-old female, demonstrated successful surgical removal with histopathological confirmation of a benign schwannoma. These cases underscore the importance of tailored management strategies based on thorough clinical, radiographic, and histopathological assessments to optimize outcomes and minimize risks associated with surgical intervention.
Clinical correlates and pathology of non-diabetic renal disease in diabetes mellitus
Authors: Antoniammal Josephin , Idiculla Jyothi , Chandy Sara
Published: October 2024

Objectives:

Early identification and differentiation of patients with non-diabetic renal disease (NDRD) from diabetic nephropathy (DN) or those with NDRD superimposed on DN improves the prognosis and reduces associated morbidity. The objectives of the study were to compare the clinical profile, nature of renal involvement, and etiopathogenesis (renal biopsy) of patients with isolated NDRD and NDRD superimposed on DN.

Materials and Methods:

It is a descriptive study in patients with T2D and renal involvement suggestive of non-diabetic etiology further evaluated with renal biopsy and grouped as NDRD alone or NDRD with DN.

Results:

Of the total 50, 66% were male, the mean age was 55.57 ± 12.28 years, and all were proteinuric. Overall, isolated NDRD and NDRD superimposed on DN were observed in 64% and 36% of patients, respectively. Diabetic retinopathy was absent in 82% of cases. The most common finding in isolated NDRD was membranous nephropathy, followed by immunoglobulin A (IgA) nephropathy and rapid progression of glomerular nephritis (RPGN). At the same time, in the NDRD and DN group, maximum patients displayed IgA nephropathy followed by acute tubular necrosis and RPGN. The incidence of atypical features of renal disease was almost twice as high in the isolated NDRD group than in the group with both NDRD + DN.

Conclusion:

NDRDs are highly prevalent, and DN may superimpose these. Recognizing NDRD solely on the basis of clinical indicators is challenging. Therefore, histopathological analysis seems essential to accurately diagnose NDRD in diabetic patients to reduce the probability of missed NDRD diagnosis and initiate prompt treatment.
ИССЛЕДОВАНИЕ ТЕХНОЛОГИИ БЕЗОПАСНОЙ ОТРАБОТКИ ЦЕЛИКОВ УГЛЯ ПРИ КАМЕРНО-СТОЛБОВОЙ СИСТЕМЕВ ЗАРУБЕЖНЫХ ШАХТАХ
Authors: Семенцов В.В. , Осминин Д.В. , Добровольский М.С. , Нифанов Е.В. , Прокопенко С.А.
Published: October 2024

Камерно-столбовая отработка (КСО) угольных пластов в российских шахтах используется на участках, непригодных для разработки длинными столбами, тогда как в зарубежных шахтах она применяется как основная с высокими технико-экономическими показателями. Изучение возможностей расширения области применения КСО в отечественных условиях определяет необходимость углубленного изучения зарубежных технологий угледобычи высокого уровня эффективности.Рассмотрены технологические схемы отработки целиков угля при камерно-столбовой системе разработки угольных пластов в шахтах США, Австралии, ЮАР и т. д. Описана конструкция самоходных крепей для управления кровлей на этапе доработки угольных панелей. Изучено влияние самоходных крепей на безопасность и эффективность отработки целиков. Применение прогрессивного оборудования позволяет довести уровень извлечения угля до 90 % и более.

Chamber-pillar mining (CSR) of coal beds in Russian mines is used in areas unsuitable for development by long pillars, while in foreign mines it is used as the main one with high technical and economic indicators. The research of possibilities of expanding the field of CSR application in domestic conditions determines the need for an in-depth research of foreign coal mining technologies of the high level of efficiency.Technological schemes of development of coal pillars at a chamber-pillar system of coal bed development in mines of USA, Australia, South Africa, etc., are considered. Design of self-propelled supports for roof control at stage of coal panels rework is described. The influence of self-propelled supports on safety and efficiency of pillar recovery has been studied. The use of advanced equipment allows to bring the level of coal extraction to 90 % or more.
МЕТОД ПРОГНОЗА ГОРНО-ГЕОЛОГИЧЕСКИХ УСЛОВИЙ НА ОСНОВЕ АНАЛИЗА РЕЗУЛЬТАТОВ КОМПЬЮТЕРНОГО ЭКСПЕРИМЕНТА С ЦИФРОВОЙ МОДЕЛЬЮ МАССИВА ГОРНЫХ ПОРОД
Authors: Игнатов Ю.М. , Гагарин А.А. , Роут Г.Н.
Published: October 2024

В статье приводятся результаты работ по созданию методики компьютерного моделирования пластовых месторождений. Цель компьютерного моделирования - разработка методов информационного обеспечения принятия проектных решений на основе применения геоинформационных технологий.Приведены основные используемые понятия, структура компьютерного моделирования и метод прогноза горно-геологических условий, основанный на анализе результатов компьютерного эксперимента с моделью массива горных пород. Метод позволяет производить поиск закономерностей в волновой структуре полей геообъектов и использовать установленные зависимости для повышения точности построения границ опасных зон. Представлены результаты применения метода прогноза горно-геологических условий выемочного столба 66-09 Шахты «Талдинская-Западная-1».

The article presents the results of work on the creation of a computer modeling method of reservoir deposits. The purpose of computer modeling is to develop methods of information support for project decision-making based on the use of geoinformation technologies. The basic concepts used the structure of computer modeling and the method of prediction of mining and geological conditions based on the analysis of the results of a computer experiment with a model of a rock mass are presented. The method makes it possible to search for regularities in the wave structure of fields of geo objects and use the established dependencies to improve the accuracy of the construction of the boundaries of dangerous zones. The results of the application of the forecast method of mining and geological conditions of the excavation column 66-09 of the Taldinskaya-Zapadnaya-1 mine are presented.
АНАЛИЗ ГЕОТЕХНОЛОГИЧЕСКИХ СТРУКТУР ОТКРЫТО-ПОДЗЕМНОЙ РАЗРАБОТКИ УГОЛЬНЫХ МЕСТОРОЖДЕНИЙ КУЗБАССА
Authors: Федорин В.А. , Шахматов В.Я. , Шишков Р.И.
Published: October 2024

Рассмотрена технология отработки пологих пластов угольных месторождений открыто-подземным способом с продольно-поперечной системой вскрытия разреза в совокупности с применением шахтных участков. Приведено описание и ключевые характеристики технологических решений освоения угольных месторождений открытым и комбинированным способами.

The technology of shallow seams mining of coal deposits by the open-underground method with a longitudinal-transverse system of opening the section in combination with the use of mine sites is considered. The paper provides a description and key characteristics technological solutions for the development of coal deposits by open and combined methods.
ПОДАВЛЕНИЕ И ЛОКАЛИЗАЦИЯ ВЗРЫВНОГО ГОРЕНИЯ РУДНИЧНЫХ ГАЗОВ С ИСПОЛЬЗОВАНИЕМ АЭРОДИНАМИЧЕСКОГО ПРИНЦИПАУСТРАНЕНИЯ ВОЗГОРАНИЯ
Authors: Ботвенко Д.В. , Казанцев В.Г.
Published: October 2024

Приведены основные принципы по подавлению и локализации взрывного горения рудничных газов, основанные на изменения скорости потока газовоздушной смеси взрывчатой концентрации.

Basic principles on suppression and confinement of mine gases explosive combustion based on changes in flow rate of gas-air mixture of explosive concentration are given.
МЕТОДИКА ОЦЕНКИ СТЕПЕНИ ВЛИЯНИЯ ПОДЗЕМНЫХ ГОРНЫХ РАБОТ НА ТЕХНИЧЕСКОЕ СОСТОЯНИЕ ЖИЛЫХ ДОМОВ И ПРИРОДНЫХ ОБЪЕКТОВ
Authors: Степанов Ю.А. , Шестаков А.О.
Published: October 2024

В статье описывается методика оценки степени влияния подземных горных работ на техническое состояние жилых домов, расположенных на подрабатываемых территориях. Предлагаемая методика позволяет производить оценку влияния подземных горных работ без проведения непрерывного мониторинга за техническим состоянием жилого дома. Основными исходными данными являются максимальные горизонтальные деформации от каждой очистной выработки, в зоне влияния которой располагается жилой дом, и предельные горизонтальные деформации для жилого дома, а также фактический и нормативный усреднённый сроки службы жилого дома. На основе полученных результатов можно определять комплекс мероприятий для охраны сооружений и природных объектов от вредного влияния подземных горных разработок на угольных месторождениях Кузбасса.

The article describes the methodology for assessing the degree of influence of underground mining operations on the technical condition of dwelling houses located in the mined areas. The proposed methodology allows to assess the impact of underground mining operations, without continuous of the technical condition monitoring of a dwelling houses. The main initial data are the maximum horizontal deformations from each treatment plant, in the influence area of which the dwelling houses is located, and the maximum horizontal deformations for the dwelling houses, as well as the actual and normative expected life of the dwelling houses. Based on the results obtained, a set of measures can be determined
ПРЕДЛОЖЕНИЯ ПО СОВЕРШЕНСТВОВАНИЮ УПРАВЛЕНИЯ ГАЗОВЫДЕЛЕНИЕМ ПРИ ВЕДЕНИИ ПОДЗЕМНЫХ ГОРНЫХ РАБОТ
Authors: КОЗЫРЕВА Е.Н.
Published: October 2024

Разработаны методы прогноза метанообильности подготовительных выработок и выемочных участков в условиях изменчивости свойств угольных пластов и состояний массива горных пород при техногенном воздействии и с учётом горно-технологических условий проведения выработок. Представлены методы повышения эффективности управления газовыделением при ведении подземных горных работ с целью обеспечения безопасных (по газовому фактору) режимов работы забоев.

Methods have been developed to predict the methane abundance of preparatory workings and excavation areas in conditions of variability of coal seams properties and rock mass states under man-made impact and taking into account mining and technological conditions of workings. Methods are presented to increase efficiency of gas release control during underground mining operations in order to ensure safe (gas-related) conditions of face operation.
ОСОБЕННОСТИ ВЫПОЛНЕНИЯ ЗАМЕРОВ ГАЗОНОСНОСТИ УГОЛЬНОГО ПЛАСТА ПРИ ПРОВЕДЕНИИ ПОДГОТОВИТЕЛЬНЫХ ВЫРАБОТОК
Authors: ПЛАКСИН М.С.
Published: October 2024

В статье рассматриваются технологические особенности выполнения замеров газоносности угольного пласта при проведении подготовительной выработки. Данные о метанообильности выработки предлагается использовать как дополнительный параметр для мониторинга «внезапного» расширения зоны опорного неупругого деформирования вследствие изменения коэффициента прочности угольного пласта.

The article deals with technological peculiarities of coal bed gas-bearing measurements during preparatory working. It is proposed to use the methane abundance data as an additional parameter for monitoring the «sudden» expansion of the support inelastic deformation zone due to a change in the strength coefficient of the coal seam.
ЭКОЛОГИЯ И БЕЗОПАСНОСТЬ - ПРИОРИТЕТЫ МАНЭБ!
Authors: Голик А.С. , Попов В.Б. , Дружинин А.А. , Власов В.В. , Кравченко С.Н.
Published: October 2024

Изложены структура и основные направления деятельности Международной академии наук экологии и безопасности жизнедеятельности (МАНЭБ), являющейся ассоциированным членом Департамента общественной информации (DPI) и Экономического и социального комитета (ECOSOC) Организации Объединенных Наций (UN). Указано, что общее число зарубежных и российских территориальных отделений МАНЭБ, активно участвующих в выполняемых Академией проектах, к настоящему моменту достигло 101. В связи с особым статусом Кузбасса в экономике страны в 1998 году по инициативе Президента МАНЭБ в г. Кемерово был организован филиал Академии, который в 2001 году решением Президиума МАНЭБ от 25.02.2001 № 83 преобразован в Региональное Сибирское отделение (РСО) МАНЭБ.

The structure and main activities of the International Academy of Environmental Sciences and Life Safety (IESCO), an associate member of the United Nations Department of Public Information (DPI) and the Economic and Social Committee (ECOSOC), are outlined. It is stated that the total number of foreign and Russian territorial branches of IESCO actively participating in projects implemented by the Academy has now reached 101. Due to the special status of Kuzbass in national economy in 1998 at the initiative of the President of IESCO in Kemerovo the branch of Academy which in 2001 is transformed by the decision of Presidium of IESCO of 25.02.2001 No. 83 to the Regional Siberian Office (RSO) of IESCO was organized.