bluuebunny/crossref_metadata_2025 huggingface dataset:
Language Interfaces in Adult Heritage Language Acquisition: A Study on Encoding of Nominal Reference in Mandarin Chinese as a Heritage Language
Authors: Jin Jing , Ke Sihui Echo , Lee John Chi-Kin
Published: January 2022

According to the Interface Hypothesis in the field of bilingualism, the interface connecting a linguistic module with a language-external domain (e.g., syntax-discourse) will present prolonged difficulties for adult bilingual learners, as compared with the interface connecting language-internal modules (e.g., syntax-semantics). This study tested whether the Interface Hypothesis is applicable to the acquisition of Mandarin Chinese as a heritage language. An internet-based acceptability judgment task (AJT) was administered to 58 advanced and intermediate adult Chinese heritage speakers to collect data in accuracy and reaction time to investigate the adult heritage speakers’ mastery of referential nominal expressions regulated at the syntax-semantics and syntax-discourse interfaces, respectively, in Mandarin Chinese. The target linguistic phenomena involved three nominal expressions (i.e., the bare N(oun), the [Cl(assifier)-N], and the [Num(eral)-Cl-N]) under four interface-regulated referential readings (i.e., type-denoting, quantity-denoting, indefinite individual-denoting, and definite individual-denoting). In terms of accuracy, the results showed that (i) for the N and the [Num-Cl-N], regardless of the interface type, the advanced group acquired the target phenomena to a nativelike level, who significantly outperformed the intermediate group; (ii) for the [Cl-N], the advanced group exhibited nativelike attainment at the syntax-discourse interface but not at the syntax-semantics interface, and performed significantly better than the intermediate group at both interfaces. Regarding reaction time, no significant differences were reported between the advanced group and the native group for the target structures at either the syntax-semantics or the syntax-discourse interface, while the advanced group performed significantly better than the intermediate group, regardless of the interface type and the structure type. The findings suggest that the nature of the language interface, i.e., whether it pertains to language-external domains (i.e., the external interface) or not (i.e., the internal interface), should not be a reliable factor for predicting the (im)possibility of nativelike attainment of bilingual grammar knowledge, contra the predictions of the Interface Hypothesis. The present study provides new empirical evidence to show that language-external interface properties are not necessarily destined for prolonged difficulties in heritage language acquisition, and that it is possible for adult heritage speakers to make developmental progress in both accuracy and processing efficiency at different types of interfaces.
The Influence of English as a Foreign Language Teachers’ Positive Mood and Hope on Their Academic Buoyancy: A Theoretical Review
Authors: Dong Hui , Li Wei , Ye Di
Published: January 2022

Emotions are now considered critical elements of a successful education. In English as a Foreign Language (EFL) context, there are many challenges for teachers to deal with. Hence, it is necessary to take their emotions into consideration. Despite many studies in this area, researching teachers’ positive mood, hope, and academic buoyancy has been left less attended. Trying to introduce this line, the present study reviewed the definitions, related concepts, theories, and previous studies done on these three variables in detail. It also touched upon the origins of researching emotion in educational contexts describing different schools of psychology. Additionally, the study offered some practical implications for EFL teachers, students, policy-makers, teacher trainers, and researchers. Finally, it enumerated the existing gaps in this area and made a number of research suggestions for future research.
The Predictive Effects of Gender and Academic Discipline on Foreign Language Enjoyment of Chinese High School Students
Authors: Huang Jian , Jiang Guiying
Published: January 2022

Foreign Language Enjoyment (FLE) plays a facilitative role in FL learning and its contributing factors have been the object of scholarly attention in the Positive Psychology approach to second language acquisition (SLA). The present study examined the predictive effects of gender and academic discipline on overall FLE and each of its subcomponents in a specific Chinese EFL context. Statistical analyses based on a sample of 1,718 high school students showed that: (1) female students scored significantly higher in overall FLE,FLE-Private, andFLE-Atmospherethan their male counterparts, but no significant difference was found in theirFLE-Teacher; (2) students of Humanities and Social Sciences scored significantly higher in overall FLE,FLE-TeacherandFLE-Atmospherethan students of Natural Science, though no significant difference was identified forFLE-Private. Reasons for the statistical variations and their implications were also discussed.
Knowledge, Attitudes, Risk Perceptions, and Practices of Spanish Adolescents Toward the COVID-19 Pandemic: Validation and Results of the Spanish Version of the Questionnaire
Authors: Aguilar-Latorre Alejandra , Asensio-Martínez Ángela , García-Sanz Olga , Oliván-Blázquez Bárbara
Published: January 2022

Background: Adolescence is a period with physical, psychological, biological, intellectual, and social changes in which there is usually little perception of risk. COVID-19 has generated constant situations of change and uncertainty worldwide. During the pandemic, the acquisition of preventive behaviors has been relevant. Various studies carried out with adults associate risk perception and the implementation of preventive behaviors with knowledge about the COVID-19 and with age, but there are not many studies with adolescents. Therefore, the objective is to validate, in Spanish, the questionnaire of the knowledge, attitudes, risk perceptions, and practices of adolescents toward the pandemic, and analyze it according to sociodemographic characteristics.

Method: This study was a descriptive cross-sectional study, which included adolescents between the ages of 12–18 (n = 354). First, a translation and a back-translation of the questionnaire were performed. The questionnaire was presented in several high schools chosen by convenience sampling and following a non-probabilistic snowball sampling. Reliability and validity analyses were then carried out and the relationships between the different sociodemographic variables (gender, place of residence, level of education, if the person was in a sentimental relationship, and financial aid) were analyzed.

Results: The reliability of the questionnaire is acceptable (ordinal alpha = 77%). Knowledge was higher in women, and in those with a higher level of education; and were lower in those who lived in smaller towns, as well as in those who had a member of their family receiving financial aid. In terms of attitudes and risk perceptions, younger adolescents had higher scores, and those who had a member of their family receiving financial aid, lower.

Conclusion: The questionnaire is a reliable tool in the Spanish adolescent population. Knowledge was influenced by gender, place of residence, level of education, and financial aid. Attitudes and risk perceptions were influenced by age and financial aid. For practices, no predictors were found. In general, adolescents scored lower on knowledge about COVID-19, but they scored higher on COVID-19 safety practices.
Allergic Rhinitis and Depression: Profile and Proposal
Authors: Mou Ya-Kui , Wang Han-Rui , Zhang Wen-Bin , Zhang Yu , Ren Chao , Song Xi-Cheng
Published: January 2022

In addition to nasal symptoms, patients with allergic rhinitis (AR) often experience mental and psychological disorders such as depression. Depression not only makes the treatment of AR more difficult and expensive but also poses a serious impact on the patients' daily activities and quality of life, thus bringing additional burden to the families and the society. Here we systematically review the recent research advances in the correlation between AR and depression, analyze the possible causes and mechanisms of depression in AR, summarize the current diagnosis and treatment strategies, and provide our insights into the AR-related depression; in addition, we introduce briefly the basic research status on AR-related depression. We hope that this review article will provide evidence for future studies.
Anxiety and Depression Prevalence and Risk Factors Among Patients With Cardiovascular Diseases in Post-COVID-19 China
Authors: Wu Minglan , Shen Liying , Wang Qiqi , Liu Li , Lu Sen , Jin Jianmei , Dai Zhen , Shu Zheyue
Published: January 2022

Objective: Data are limited on the psychological disorders of patients with cardiovascular disease during the post-COVID-19 period, although mental health status is associated with morbidity and mortality. We aimed to investigate the prevalence of anxiety and depression and risk factors among patients with cardiovascular disease in the post-pandemic period.

Method: A cross-sectional survey was conducted through opportunistic and snowball sampling in southeast China from 10 October to 24 November. Anxiety and depression were assessed on the hospital anxiety and depression scale (HADS).

Results: A total of 435 patients with hypertension (48.05%), atrial fibrillation (17.24%), coronary artery disease (14.48%), heart failure (9.89%) and other heart diseases (10.34%) completed the survey. Interestingly, most patients reported monthly income comparable to (90.11%) or even greater than (8.51%) pre-pandemic income. The occurrence of anxiety and depression was 11.72 and 9.20%, respectively. Marital status and treatment interruption during the pandemic were independent risk factors for both anxiety and depression. Moreover, current monthly income and access to telemedicine during the pandemic were independent risk factors for anxiety.

Conclusion: Patients with cardiovascular disease may experience anxiety and depression not only because of disease complications but also because of the effects of the pandemic. In facing the global challenge posed by the coronavirus, efforts should be made to improve patients' psychological well-being in the management of populations with cardiovascular disease.
Psychological Distress During COVID-19 Curfews and Social Distancing in Saudi Arabia: A Cross-Sectional Study
Authors: Alghamdi Badrah S. , Alatawi Yasser , Alshehri Fahad S. , Tayeb Haythum O. , AboTaleb Hanin , Binsalman Amal
Published: January 2022

Background: Coronavirus disease 2019 (COVID-19) has spread to over 150 countries worldwide. Since the first case of COVID-19 was confirmed in Saudi Arabia, cases have continued to escalate exponentially. The COVID-19 outbreak has had a negative effect on mental health and well-being. The study aimed to investigate the effects of the strict national regulations associated with the COVID-19 pandemic on mental health.

Methods: This was a cross-sectional study of a convenience sample of Saudi residents. Saudi residents aged 18 years or older were invited to complete an online questionnaire after one month of a nationwide 24-h curfew between May 6, 2020 and May 13, 2020. We measured psychological distress using the Depression, Anxiety, and Stress Scale-21 (DASS-21). We ran binary logistic regression analyses to detect variables that significantly predicted DASS-21 scores.

Results: A sample of 2252 participants was recruited from the general population of Saudi Arabia. The DASS-21 score means and standard deviations for depression and anxiety for the whole sample (10.73 ± 10.29 and 6.98 ± 8.30, respectively) were in the range of mild depression and anxiety. In contrast, the mean DASS-21 stress score was within the normal range (11.97 ± 10.80). The mean stress score for healthcare workers was within the normal range (13.70 ± 10.68) but was significantly higher than the mean score for the public (11.56 ± 10.89; P = 0.0006). Several variables (e.g., age, gender, and history of contact with confirmed COVID-19 cases) were significantly associated with higher DASS-21 scores.

Conclusions: The COVID-19 pandemic has created a psychological burden. Therefore, there is an urgent need to implement emergency public health interventions that ameliorate the risk perception of COVID-19 through the dissemination of adequate and targeted health information that could be a successful measure to mitigate the psychological impact of the Covid-19 pandemic.
Remarks on Multimodality: Grammatical Interactions in the Parallel Architecture
Authors: Cohn Neil , Schilperoord Joost
Published: January 2022

Language is typically embedded in multimodal communication, yet models of linguistic competence do not often incorporate this complexity. Meanwhile, speech, gesture, and/or pictures are each considered as indivisible components of multimodal messages. Here, we argue that multimodality should not be characterized by whole interacting behaviors, but by interactions of similar substructures which permeate across expressive behaviors. These structures comprise a unified architecture and align within Jackendoff's Parallel Architecture: a modality, meaning, and grammar. Because this tripartite architecture persists across modalities, interactions can manifest within each of these substructures. Interactions between modalities alone create correspondences in time (ex. speech with gesture) or space (ex. writing with pictures) of the sensory signals, while multimodal meaning-making balances how modalities carry “semantic weight” for the gist of the whole expression. Here we focus primarily on interactions between grammars, which contrast across two variables: symmetry, related to the complexity of the grammars, and allocation, related to the relative independence of interacting grammars. While independent allocations keep grammars separate, substitutive allocation inserts expressions from one grammar into those of another. We show that substitution operates in interactions between all three natural modalities (vocal, bodily, graphic), and also in unimodal contexts within and between languages, as in codeswitching. Altogether, we argue that unimodal and multimodal expressions arise as emergent interactive states from a unified cognitive architecture, heralding a reconsideration of the “language faculty” itself.
Impact of Extreme Temperature and Soil Water Stress on the Growth and Yield of Soybean (Glycine max (L.) Merrill)
Authors: Ogunkanmi Labake , MacCarthy Dilys S. , Adiku Samuel G. K.
Published: January 2022

Climate change is a major environmental stressor that would adversely affect tropical agriculture, which is largely rain-fed. Associated with climate change is an increasing trend in temperature and decline in rainfall, leading to prolonged and repeated droughts. The purpose of this study was to determine the effect of climate variables such as temperature, relative humidity, vapor pressure deficit (VPD), and soil water on the phenology, biomass, and grain yield of soybean crops. A greenhouse experiment was set in a split plot design with three average environmental conditions as the main plots: E1 (36 °C, RH = 55%), E2 (34 °C, RH = 57%) and E3 (33 °C, RH = 44%). Additionally, there were three water treatments: W1 (near saturation), W2 (Field capacity), and W3 (soil water deficit) and two soybean varieties (Afayak and Jenguma). These treatments were replicated nine times. The results showed that high temperatures (E1) accelerated the crop development, particularly at flowering. Additionally, increased atmospheric demand for water under a high temperature environment resulted in high evapotranspiration, leading to high transpiration which probably reduced photosynthetic activity of the plants and thereby contributing to biomass and grain yield loss. Biomass and yield were drastically reduced for the combined effect of high temperature (E1) and drought (W3) as compared to combined effect of ambient temperature (E3) and well-watered condition (W1). Increasing temperatures and erratic rainfall distributions associated with climate change poses a potential threat to the soybean production in Ghana.
Supporting the Bidding Decisions of Smallholder Farmers in Public Calls in Brazil
Authors: Alves Junior Paulo Nocera , Costa Melo Isotilia , Yamanaka Lie , Severino Maico Roris , Rentizelas Athanasios
Published: January 2022

In Brazil, the National School Feeding Program (PNAE) seeks to contribute to the socio-economic development of smallholder farmers, prioritizing them in supplying their products for preparing daily meals in public schools. However, farmers face challenges in determining which school calls to bid for and the potential benefits from their participation, due to the multiple quantitative and qualitative decision criteria involved. This paper presents a novel Data Envelopment Analysis (DEA)-based method for bidding priority setting, to support the decision making. The model was applied for a case study in Brazil. The academic contribution lies in the innovation of using a Double-Frontier Slack-Based Measure (SBM) DEA model for Hierarchical Network systems, i.e., applied to multiple levels and followed by a tie-breaking method. The practical contribution lies in the decision support of farmers by presenting the results at three levels, the first of which is a ranking by the town or urban cluster priority, the second by the school, and the third by the products. Thus, using the rankings of calls, farmers can make informed decisions regarding the feasibility of bidding for each PNAE public call. At the same time, the objective rankings can alleviate friction and conflict within co-operatives during the decision-making process.
Intercropping Pattern and N Fertilizer Schedule Affect the Performance of Additively Intercropped Maize and Forage Cowpea in the Mediterranean Region
Authors: Salama Heba S. A. , Nawar Ali I. , Khalil Hassan E.
Published: January 2022

Intercropping maize and forage cowpea is a widely proposed strategy to improve land use efficiency, and maximize the economic value of the farming system, especially in developing countries with restricted resources. The current study was carried out during the successive summers of 2020 and 2021 in Northern Egypt. The main objective was to evaluate the effect of three N schedules (NS1, NS2, NS3), when three different maize–cowpea intercropping patterns (IP1, IP2, IP3) were applied, on the grain yield of maize, forage yield and quality of forage cowpea. In addition, yield gain and land use efficiency were evaluated using the land equivalent ratio (LER) and dry matter equivalent ratio (DMER) indices. Results revealed that the intercropping patterns that provided wider spacings for the component crops and reduced the competition between them, mainly IP3, resulted in the best performances for the two crops. This was clear for maize ear and grain yields, 100-grain weight and harvest index, in addition to cowpea fresh and dry forage yields, crude protein and non-fiber carbohydrates of the three cuts. Regarding the applied N schedules, NS1 which included the application of a N starter dose with sowing proved to be the most efficient schedule that led to the best performance for both crops. Maize produced 9.07 t ha−1 grain yield under IP3 and NS1. In addition, the application of IP3 resulted in the highest significant cowpea dry forage yield (DFY), with the highest crude protein (CP) content. The DFY of cuts 1, 2, and 3 amounted to 1.27, 0.45, and 0.24 t ha−1, while the CP content for the three respective cuts reached 159.49, 157.96, and 148.91 g kg−1. Nonetheless, NS1 produced a reasonable amount of DFY with high CP content. It is recommended to follow the third proposed intercropping pattern (IP3) and to include a nitrogen starter dose (NS1) in the fertilization scheme to ensure highest productivity from the intercropped maize and forage cowpea.
Evaluation of the DNDC Model to Estimate Soil Parameters, Crop Yield and Nitrous Oxide Emissions for Alternative Long-Term Multi-Cropping Systems in the North China Plain
Authors: Abdalla Mohamed , Song Xiaotong , Ju Xiaotang , Smith Pete
Published: January 2022

Optimizing crop rotations is one of the proposed sustainable management strategies for increasing carbon sequestration. The main aim of this study was to evaluate the DeNitrification-DeComposition (DNDC) model for estimating soil parameters (temperature, moisture and exchangeable NO3− and NH4+), crop yield and nitrous oxide (N2O) emissions for long-term multi-cropping systems in Hebei, China. The model was validated using five years of data of soil parameters, crop yields and N2O emissions. The DNDC model effectively simulated daily soil temperature, cumulative soil nitrogen and crop yields of all crops. It predicted the trends of observed daily N2O emissions and their cumulative values well but overestimated the magnitude of some peaks. However, the model underestimated daily water filled pore space, especially in dry seasons, and had difficulties in correctly estimating daily exchangeable NO3− and NH4+. Both observed and simulated cumulative N2O results showed that optimized and alternative cropping systems used less nitrogen fertiliser, increased grain yield and decreased N2O emissions compared to the conventional cropping system. Our study shows that although the DNDC model (v. 9.5) is not perfect in estimating daily N2O emissions for these long-term multi-cropping systems, it could still be an effective tool for predicting cumulative emissions.
Productivity of Rainfed Winter Wheat with Direct Sowing and Economic Efficiency of Diversified Fertilization in Arid Region of South Kazakhstan
Authors: Turebayeva Sagadat , Zhapparova Aigul , Yerkin Akbota , Aisakulova Khaiyrnisa , Yesseyeva Gainiya , Bissembayev Anuarbek , Saljnikov Elmira
Published: January 2022

Reduced soil tillage is a powerful means to mitigate soil degradation. However, in arid climates, no-till rainfed technologies often result in yield drop due to lack of soil moisture and mineral nutrition. Rainfed production of winter wheat using direct sowing and diversified fertilization in South Kazakhstan was studied in 2019–2020. Eight field-scale treatments using nitrogen and phosphorus fertilizers were studied for biometric parameters of winter wheat. An economic profitability of the amendments used was assessed. The soil managed to accumulate productive moisture to support plants’ needs during formation of productive organs. Use of phosphorus fertilizer at direct sowing accelerated grain maturation, and the combination of phosphorus and nitrogen fertilizer lengthened the growing season. The highest production cost of 1 tonne of grain was in the plots that did not receive any amendments, and the lowest cost was in the treatment with use of plant growth stimulants together with micronutrient fertilizer. For the first time, optimization of the soil nutritional regime with consideration of the biological and phenological demands allowed for the balance of the plant nutrition and cost efficiency of grain production with direct sowing of winter wheat in dry conditions in South Kazakhstan.
The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation
Authors: Kim Eunji , Almubarak Areeg , Talha Nabeel , Yu Il-Jeoung , Jeon Yubyeol
Published: January 2022

κ-Carrageenan is a plant polysaccharide derived from red seaweeds reported to possess potential medicinal and antioxidants activities. The present study aimed to identify the cryoprotective effects of κ-carrageenan on the quality of frozen-thawed canine semen. Twenty-eight ejaculates were collected and diluted in a Tris egg-yolk-free extender supplemented with various concentrations of κ-carrageenan (0.0%, 0.1%, 0.2%, 0.3%, and 0.5%). The addition of κ-carrageenan to the extender at a 0.2% concentration induced a significant increase in the total motility (TM) and the rapid progressive motility (RPM) of canine sperm. Among the experimental groups, the highest percentage of sperms with intact acrosomes was found in the 0.5% κ-carrageenan group (p < 0.05). Apoptosis levels were significantly lower in the 0.1% and 0.2% κ-carrageenan treatment. Moreover, sperm in the κ-carrageenan supplemented group showed a significantly higher expression of antiapoptotic (Bcl-2) and lower expression of NADPH oxidase (NOX5), spermine synthase (SMS), and spermine oxidase (SMOX) genes than those in the control group. In conclusion, the addition of κ-carrageenan to the freezing extender improved the overall efficiency of frozen-thawed dog spermatozoa.
Selected Quality Attributes of Freshwater Mussel Powder as a Promising Ingredient for Pet Food
Authors: Konieczny Piotr , Andrzejewski Wojciech , Yang Tianyu , Urbańska Maria , Stangierski Jerzy , Tomczyk Łukasz , Mikołajczak Beata
Published: January 2022

The aim of this study was to describe the quality attributes of a freeze-dried preparation obtained from freshwater mussel Sinanodonta woodiana (SW) soft tissue in respect to its potential as a novel pet food ingredient. After ecotoxicological testing of the raw material with MARA (Microbial Assay for Risk Assessment), the basic physico-chemical properties of the powder, such as approximate composition, bulk density, color parameters, water activity, electrophoretic analysis (SDS-PAGE), solubility, gelling and emulsifying capacity, were analyzed. The powder with a water activity of 0.43 offers a toxically safe preparation that contains over 34% protein/100 g of dry matter (DM). The SDS-PAGE profile showed twelve protein bands with a molecular weight (MW) ranging from >250 to 10 kDa. Taurine content has been estimated at an essential amount above 150 mg/100 g of DM. The powder possessed desirable emulsifying properties with 230 mL per 1 g and demonstrated the ability to form a firmer gel with a strength of 152.9 g at a temperature above 80 °C with at least 10% protein content. The L*, a*, and b* values characterizing powder color were found to be 69.49, 16.33, and 3.86, respectively. The SW mussel powder seems to be a promising ingredient that can be added with other binding or gelling agents in order to improve both the taste and acceptance of the final pet food products.
Economic Threshold Analysis of Supplementing Dairy Cow Diets with Betaine and Fat during a Heat Challenge: A Pre- and Post-Experimental Comparison
Authors: Lewis Claire D. , Marett Leah C. , Malcolm Bill , Williams S. Richard O. , Milner Tori C. , Moate Peter J. , Ho Christie K. M.
Published: January 2022

Ex ante economic analysis can be used to establish the production threshold for a proposed experimental diet to be as profitable as the control treatment. This study reports (1) a pre-experimental economic analysis to estimate the milk production thresholds for an experiment where dietary supplements were fed to dairy cows experiencing a heat challenge, and (2) comparison of these thresholds to the milk production results of the subsequent animal experiment. The pre-experimental thresholds equated to a 1% increase in milk production for the betaine supplement, 9% increase for the fat supplement, and 11% increase for fat and betaine in combination, to achieve the same contribution to farm profit as the control diet. For the post-experimental comparison, previously modelled climate predictions were used to extrapolate the milk production results from the animal experiment over the annual hot-weather period for the dairying region in northern Victoria, Australia. Supplementing diets with fat or betaine had the potential to produce enough extra milk to exceed the production thresholds, making either supplement a profitable alternative to feeding the control diet during the hot-weather period. Feeding fat and betaine in combination failed to result in the extra milk required to justify the additional cost when compared to the control diet.
Gut Microbiota Implications for Health and Welfare in Farm Animals: A Review
Authors: Chen Siyu , Luo Shuyan , Yan Chao
Published: January 2022

In the past few decades, farm animal health and welfare have been paid increasing concern worldwide. Farm animal health and welfare are generally assessed by the measurements of physical health, immune response, behavior, and physiological indicators. The gut microbiota has been reported to have a great influence on host phenotypes, possibly via the immune processes, neural functions, and endocrine pathways, thereby influencing host phenotypes. However, there are few reviews regarding farm animals’ health and welfare status concerning the gut microbiota. In this point of view, (1) we reviewed recent studies showing that gut microbiota (higher alpha diversity, beneficial composition, and positive functions) effectively influenced health characteristics, immunity, behaviors, and stress response in farm animals (such as pigs, chickens, and cows), which would provide a novel approach to measure and evaluate the health status and welfare of farm animals. In addition, fecal microbiota transplantation (FMT) as one of the methods can modulate the recipient individual’s gut microbiota to realize the expected phenotype. Further, (2) we highlighted the application of FMT on the improvement of the production performance, the reduction in disease and abnormal behavior, as well as the attenuation of stress in farm animals. It is concluded that the gut microbiota can be scientifically used to assess and improve the welfare of farm animals. Moreover, FMT may be a helpful strategy to reduce abnormal behavior and improve stress adaption, as well as the treatment of disease for farm animals. This review suggests that gut microbiota is a promising field to evaluate and improve animal welfare.
A Cross-Sectional Study on Canine and Feline Anal Sac Disease
Authors: Corbee Ronald Jan , Woldring Hilde H. , van den Eijnde Lianne M. , Wouters Erik G. H.
Published: January 2022

Limited data are available on canine and feline non-neoplastic anal sac disease. Therefore, the aim of this study was to obtain observational data on the incidence, predisposing factors, diagnosis, treatment, and recurrence rate of canine and feline anal sac disease. To this end, a questionnaire was distributed among veterinarians. The incidence of non-neoplastic anal sac disease was estimated at 15.7% in dogs and 0.4% in cats. Predisposing factors were diarrhea, skin problems, several dog breeds, and particularly small breed dogs, male cats, British shorthairs, and obesity in dogs. Diagnosis was made based on the presence of clinical signs and characteristics of the anal sac content. Manual expression and treating any potential underlying disease were the most important treatments for all three types of non-neoplastic anal sac disease. Anal sacculectomy was performed in refractory cases. The most recurrent anal sac disease condition was impaction. Diagnosis of anal sac disease should be based on clinical signs and rectal examination, as the evaluation of the anal sac content is not reliable. Surgical outcomes of anal sacculectomy can be improved when surgery is performed after medical management. Future studies should investigate these findings in prospective trials.
Fatty Acid Profile, Conjugated Linoleic Acid Content, and Lipid Quality Indices in Selected Yogurts Available on the Polish Market
Authors: Paszczyk Beata , Czarnowska-Kujawska Marta
Published: January 2022

The aim of the present study was to determine the fatty acid composition, the content of cis9trans11 C18:2 acid (CLA), and lipid quality indices in yogurts made of cow’s milk, available on the Polish market. The test material consisted of: natural yogurts, natural yogurts with additives (muesli, cereal grains), bio yogurts, bio yogurts with additives (millet groats, quinoa, chestnuts), probiotic yogurts, and eco yogurts. All the products were bought in the period from May to June 2021. The conducted research showed that the analyzed yoghurts were characterized by a varying content of fatty acid groups, different values of the calculated lipid quality indices, as well as a different content of conjugated linoleic acid cis9trans11 C18: 2 (CLA). Natural yogurts with additives had the highest content of polyunsaturated fatty acids (PUFAs) and n-3 PUFAs. Natural and bio yogurts with additives had a higher content of n-6 PUF than the other analyzed yogurts. The n-6/n-3 ratio was lower in bio yogurts and eco yogurts. Natural yogurts with additives featured the lowest index of atherogenicity (AI) and index of thrombogenicity (TI) and the highest hypocholesterolemic/hypercholesterolemic ratio (H/H). The fat extracted from the bio yogurts had the highest (0.90% of total fatty acids) mean content of cis9trans11 C18:2 (CLA). In fat of the other analyzed yogurts, mean CLA content in total content of fatty acids varied from 0.48% in natural yogurts with additives to 0.81% in bio yogurts with additives.
Virulence Gene Profile, Antimicrobial Resistance and Multilocus Sequence Typing of Salmonella enterica Subsp. enterica Serovar Enteritidis from Chickens and Chicken Products
Authors: Zakaria Zunita , Hassan Latiffah , Sharif Zawiyah , Ahmad Norazah , Mohd Ali Rohaya , Amir Husin Suraya , Mohamed Sohaimi Norfitriah , Abu Bakar Shafini , Garba Bashiru
Published: January 2022

This study was undertaken to determine the virulence, antimicrobial resistance and molecular subtypes of Salmonella in the Central Region of Peninsular Malaysia. A total of 45 Salmonella Enteritidis were detected from live chicken (cloacal swab), and chicken products (fresh and ready-to-eat meat) samples upon cultural isolation and serotyping. Similarly, an antimicrobial susceptibility test based on the Kirby Bauer disk diffusion method as well as antimicrobial resistance AMR genes, virulence determinants and multilocus sequence typing (MLST) typing were conducted after the Whole Genome Sequencing and analysis of the isolates. The results indicate that sequence types ST1925 (63.7%), and ST11 (26.5%) were the predominant out of the seven sequence types identified (ST292, ST329, ST365, ST423 and ST2132). The phenotypic antimicrobial profile corresponds to the genotypic characterization in that the majority of the isolates that exhibited tetracycline, gentamycin and aminoglycoside resistance; they also possessed the tetC and blaTEM β-Lactam resistance genes. However, isolates from cloacal swabs showed the highest number of resistance genes compared to the chicken products (fresh and ready-to-eat meat) samples. Furthermore, most of the virulence genes were found to cluster in the Salmonella pathogenicity island (SPI). In this study, all the isolates were found to possess SPI-1, which codes for the type III secretion system, which functions as actin-binding proteins (SptP and SopE). The virulence plasmid (VP) genes (spvB, spvC) were present in all genotypes except ST365. The findings of this study, particularly with regard to the molecular subtypes and AMR profiles of the Salmonella Enteritidis serotype shows multidrug-resistance features as well as genetic characteristics indicative of high pathogenicity.
Minireview: Peripheral Nesfatin-1 in Regulation of the Gut Activity—15 Years since the Discovery
Authors: Kras Katarzyna , Muszyński Siemowit , Tomaszewska Ewa , Arciszewski Marcin B.
Published: January 2022

Nesfatin-1, discovered in 2006, is an anorexigenic molecule derived from the precursor protein NEFA/nucleobindin2. It is generally postulated that this molecule acts through a specific G protein-coupled receptor, as yet unidentified. Research conducted over the last 15 years has revealed both central and peripheral actions of nesfatin-1. Given its major central role, studies determining its inhibitory effect on food intake seem to be of major scientific interest. However, in recent years a number of experiments have found that peripheral organs, including those of the gastrointestinal tract (GIT), may also be a source (possibly even the predominant source) of nesfatin-1. This mini-review aimed to summarize the current state of knowledge regarding the expression and immunoreactivity of nesfatin-1 and its possible involvement (both physiological and pathological) in the mammalian GIT. Research thus far has shown very promising abilities of nesfatin-1 to restore the balance between pro-oxidants and antioxidants, to interplay with the gut microbiota, and to alter the structure of the intestinal barrier. This necessitates more extensive research on the peripheral actions of this molecule. More in-depth knowledge of such mechanisms (especially those leading to anti-inflammatory and anti-apoptotic effects) is important for a better understanding of the involvement of nefatin-1 in GIT pathophysiological conditions and/or for future therapeutic approaches.
It’s Animals’ Ten Year Anniversary
Authors: Phillips Clive J. C.
Published: January 2022

About 30 years ago I had a discussion with my then head of department at Bangor University, the late Professor John Bryn Owen, about what an ideal journal would look like in our field, animal science, in the future [...]
Therapy of Pyothorax in Cats via Small-Bore Thoracostomy Tube in Terms of Efficacy, Complications and Outcomes
Authors: Heier Evelyn , Wurtinger Gabriel , Hassdenteufel Esther , Schneider Matthias
Published: January 2022

First-line therapy for cats with pyothorax consists of intravenous antibiotics, drainage of the septic pleural effusion and closed-chest lavage. Large-bore thoracostomy tubes are traditionally used for drainage, but case series indicate a comparable efficacy using small-bore tubes. In this retrospective study, we describe a new technique of sheath-guided small-bore (6 F) thoracostomy tubes in cats with pyothorax and evaluate their efficacy and complications. Additionally, we compare outcomes between two treatment groups. Placement and use of the small-bore thoracostomy tubes described here has a low complication rate of 4% (3/67 tubes), and 53% (24/45) of the cats could be treated with thoracostomy tubes and closed-chest lavage according to the protocol. The success rate is reduced by 18% (8/45) due to deaths caused mainly by sepsis, 16% (7/45) due to structural diseases requiring surgery and a further 14% (6/43) due to lavage failures that could only be cured after additive therapy (thoracotomy or fibrinolysis). The long-term prognosis was very good, with a survival rate one year after discharge of 94% (30/32). We detected no effect on survival by early placement of bilateral thoracostomy tubes or closed-chest lavage with a heparinised solution. In conclusion, therapy of pyothorax with small-bore thoracostomy tubes is as successful as therapy with large- or medium-bore tubes.
Immune Maturation Effects on Viral Neutralization and Avidity of Hyperimmunized Equine Anti-SARS-CoV-2 Sera
Authors: González Viacava Myriam Belén , Varese Augusto , Mazzitelli Ignacio , Lanari Laura , Ávila Lucía , García Vampa María Julia , Geffner Jorge , Cascone Osvaldo , Dokmetjian José Christian , de Roodt Adolfo Rafael , Fingermann Matías
Published: January 2022

Mass-vaccination against COVID-19 is still a distant goal for most low-to-middle income countries. The experience gained through decades producing polyclonal immunotherapeutics (such as antivenoms) in many of those countries is being redirected to develop similar products able to neutralize SARS-CoV-2 infection. In this study we analyzed the biological activity (viral neutralization or NtAb) and immunochemical properties of hyperimmune horses’ sera (HHS) obtained during initial immunization (I) and posterior re-immunization (R) cycles using the RBD domain of the SARS-CoV-2 spike protein as antigen. HHS at the end of the R cycle showed higher NtAb titers when compared to those after the I cycle (35,585 vs. 7000 mean NtAb, respectively). Moreover, this increase paralleled an increase in avidity (95.2% to 65.2% mean avidity units, respectively). The results presented herein are relevant for manufacturers of these therapeutic tools against COVID-19.
Etiology of Mastitis and Antimicrobial Resistance in Dairy Cattle Farms in the Western Part of Romania
Authors: Pascu Corina , Herman Viorel , Iancu Ionica , Costinar Luminita
Published: January 2022

The present study aimed to determine the bacteria isolated from bovine mastitis and their antimicrobial resistance in the western part of Romania. Clinical mastitis was diagnosed based on local inflammation in the udder, changes in milk, and when present, generalized symptoms. Subclinical mastitis was assessed using a rapid test—the California Mastitis Test. The identification of bacterial strains was performed based on biochemical profiles using API system tests (API 20 E, API Staph, API 20 Strep, API Coryne, API 20 NE (bioMerieux, Marcy l’Etoile, France), and MALDI-TOF mass spectrometry (MS). The prevalent isolated bacteria were Staphylococcus spp. (50/116; 43.19%), followed by Streptococcus spp. (26/116; 22.41%), E. coli (16/116; 13.79%), Corynebacterium spp. (9/116; 7.75%), Enterococcus spp. (10/116; 8.62%), and Enterobacter spp. (5/116; 4.31%). Phenotype antimicrobial resistance profiling was performed used the disc diffusion method. Generally, Gram-positive bacteria showed low susceptibility to most of the antimicrobials tested, except cephalothin. Susceptibilities to penicillins and quinolones were fairly high in Gram-negative bacteria, whereas resistance was observed to macrolides, aminoglycosides, and tetracyclines. The highest number of isolates were multidrug resistant (MDR), the resistance pathotypes identified including the most frequently antimicrobials used in cow mastitis treatment in Romania.
Effect of Working Atmospheres on the Detection of Diacetyl by Resistive SnO2 Sensor
Authors: Gnisci Andrea , Fotia Antonio , Bonaccorsi Lucio , Donato Andrea
Published: January 2022

Nanostructured metal oxide semiconductors (MOS) are considered proper candidates to develop low cost and real-time resistive sensors able to detect volatile organic compounds (VOCs), e.g., diacetyl. Small quantities of diacetyl are generally produced during the fermentation and storage of many foods and beverages, conferring a typically butter-like aroma. Since high diacetyl concentrations are undesired, its monitoring is fundamental to identify and characterize the quality of products. In this work, a tin oxide sensor (SnO2) is used to detect gaseous diacetyl. The effect of different working atmospheres (air, N2 and CO2), as well as the contemporary presence of ethanol vapors, used to reproduce the typical alcoholic fermentation environment, are evaluated. SnO2 sensor is able to detect diacetyl in all the analyzed conditions, even when an anaerobic environment is considered, showing a detection limit lower than 0.01 mg/L and response/recovery times constantly less than 50 s.
Detection of DDoS Attacks in Software Defined Networking Using Entropy
Authors: Fan Cong , Kaliyamurthy Nitheesh Murugan , Chen Shi , Jiang He , Zhou Yiwen , Campbell Carlene
Published: January 2022

Software Defined Networking (SDN) is one of the most commonly used network architectures in recent years. With the substantial increase in the number of Internet users, network security threats appear more frequently, which brings more concerns to SDN. Distributed denial of Service (DDoS) attacks are one of the most dangerous and frequent attacks in software defined networks. The traditional attack detection method using entropy has some defects such as slow attack detection and poor detection effect. In order to solve this problem, this paper proposed a method of fusion entropy, which detects attacks by measuring the randomness of network events. This method has the advantages of fast attack detection speed and obvious decrease in entropy value. The complementarity of information entropy and log energy entropy is effectively utilized. The experimental results show that the entropy value of the attack scenarios 91.25% lower than normal scenarios, which has greater advantages and significance compared with other attack detection methods.
Elderberry Stalks as a Source of High-Value Phytochemical: Essential Minerals and Lipophilic Compounds
Authors: Patinha Samuel , Murteira Juliana V. , Costa Carina Pedrosa , Salvador Ângelo C. , Santos Sónia A. O. , Silvestre Armando J. D. , Rocha Sílvia M.
Published: January 2022

Elderberry (Sambucus nigra L.) consumption has been growing in the last years, generating a large number of stalks (~10% of the berries bunch) that are still under-valorized. This study focused on the evaluation of elderberry stalks as a source of high-value phytochemicals. In this vein, the essential mineral content and lipophilic composition were analyzed for the first time. In addition, the polar fraction was evaluated regarding its total phenolic content (TPC) and antioxidant activity by both 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2-diphenyl-1-picrylhydrazyl hydrate (DPPH) assays. The lipophilic fraction was mainly composed of triterpenic acids (2902.20 mg kg−1 of dry weight (dw)), fatty acids (711.73 mg kg−1 dw) and sterols (288.56 mg kg−1 dw). Minor amounts of long-chain aliphatic alcohols and other components were also detected. Ursolic acid (2265.83 mg kg−1 dw), hexadecanoic acid (219.85 mg kg−1 dw) and β-sitosterol (202.74 mg kg−1 dw) were the major lipophilic components verified. The results of this study also indicated that elderberry stalks might be used as a natural source of essential minerals, particularly calcium, iron and potassium, which are known to play important roles in various body functions. The analysis of the polar fraction also showed that elderberry stalks present TPC as high as elderberry themselves as well as considerable antioxidant activity (1.04 and 0.37 mmol TE g−1 of extract, against respectively ABTS and DPPH radicals). These results highlight the potential of elderberry stalks as a natural source of high-value phytochemicals that may be explored in several fields.
Immobilised rGO/TiO2 Nanocomposite for Multi-Cycle Removal of Methylene Blue Dye from an Aqueous Medium
Authors: Kocijan Martina , Ćurković Lidija , Bdikin Igor , Otero-Irurueta Gonzalo , Hortigüela María J. , Gonçalves Gil , Radošević Tina , Vengust Damjan , Podlogar Matejka
Published: January 2022

This work presents the immobilisation of titanium dioxide (TiO2) nanoparticles (NPs) and reduced graphene oxide (rGO)-TiO2 nanocomposite on glass sheets for photocatalytic degradation of methylene blue (MB) under different radiation sources such as ultraviolet and simulated solar radiation. The TiO2 NPs and rGO-TiO2 nanocomposite were synthesised through a simple hydrothermal method of titanium isopropoxide precursor followed by calcination treatment. Deposition of prepared photocatalysts was performed by spin-coating method. Additionally, ethylene glycol was mixed with the prepared TiO2 NPs and rGO-TiO2 nanocomposite to enhance film adhesion on the glass surface. The photocatalytic activity under ultraviolet and simulated solar irradiation was examined. Further, the influence of different water matrices (milli-Q, river, lake, and seawater) and reactive species (h+, •OH, and e−) on the photocatalytic efficiency of the immobilised rGO/TiO2 nanocomposite was careful assessed. MB dye photocatalytic degradation was found to increase with increasing irradiation time for both irradiation sources. The immobilisation of prepared photocatalysts is very convenient for environment applications, due to easy separation and reusability, and the investigated rGO/TiO2-coated glass sheets demonstrated high efficiency in removing MB dye from an aqueous medium during five consecutive cycles.