bluuebunny/crossref_metadata_2025 huggingface dataset:
Pathway and Production Differences in Branched-Chain Hydroxy Acids as Bioactive Metabolites in Limosilactobacillus fermentum, Ligilactobacillus salivarius, and Latilactobacillus sakei
Authors: Kim Dong-Hyuk , Kim Su-Hyun , Kim Seul-Ah , Kwak Min Jeong , Han Nam Soo , Lee Choong Hwan
Published: September 2024

Branched-chain hydroxy acids (BCHAs) as bioactive metabolites of Lactobacillaceae include 2-hydroxy isovaleric acid (HIVA), 2-hydroxy isocaproic acid (HICA), and 2-hydroxy-3-methyl isovaleric acid (HMVA). Combining targeted and untargeted metabolomics, this study elucidates differences in extracellular BCHA production in Limosilactobacillus fermentum, Ligilactobacillus salivarius, and Latilactobacillus sakei alongside comparing comprehensive metabolic changes. Through targeted metabolomics, BCHA production among 38 strains exhibited strain specificity, except for L. sakei, which showed significantly lower BCHA production. Explaining the lower production in L. sakei, which lacks the branched-chain amino acid (BCAA)-utilizing pathway, comparison of BCHA production by precursor reaction revealed that the pathway utilizing BCAAs is more dominant than the pathway utilizing pyruvate. Expanding upon the targeted approach, untargeted metabolomics revealed the effects of the reaction compound on other metabolic pathways besides BCHAs. Metabolism alterations induced by BCAA reactions varied among species. Significant differences were observed in glycine, serine, and threonine metabolism, pyruvate metabolism, butanoate metabolism, and galactose metabolism (p < 0.05). These results emphasize the importance of the synergy between fermentation strains and substrates in influencing nutritional components of fermented foods. By uncovering novel aspects of BCAA metabolism pathways, this study could inform the selection of fermentation strains and support the targeted production of BCHAs.
Novel Molecular Mechanisms Underlying the Ameliorative Effect of Platelet-Rich Plasma against Electron Radiation-Induced Premature Ovarian Failure
Authors: Demyashkin Grigory , Vadyukhin Matvey , Murtazalieva Zaira , Pugacheva Ekaterina , Schekin Vladimir , Bimurzaeva Makka , Pesegova Svetlana , Shegay Petr , Kaprin Andrey
Published: September 2024

Radiotherapy is one of the risk factors for radiation-induced premature ovarian failure and infertility in cancer patients. The development of methods for ovarian radioprotection remains relevant. Moreover, electrons are a little-studied and promising method of radiation with the least toxic effect on normal tissues. The assessment of intracellular mechanisms regulating the protective effects of leukocyte-poor platelet-rich plasma in a model of radiation-induced premature ovarian failure caused by electron irradiation. Wistar rats were divided into four groups, namely a control group, irradiation group (electron exposure), irradiation + leukocyte-poor platelet-rich plasma group, and only leukocyte-poor platelet-rich plasma group. Fragments of ovaries were removed and hormonal, oxidant, histological, and morphometric studies were carried out. The cell cycle of ovarian follicles and the inflammatory and vascular response were assessed using immunohistochemistry. The activity of MAPK, ERK, and PI3K pathways was also assessed using the RT-qPCR. We found that electron irradiation causes a decrease in the functional activity of the ovaries and the death of follicular cells through apoptosis. The administration of LP-PRP led to a partial restoration of the cytokine balance. In addition, minor ovarian damage and mild inflammation were observed in this group. Leukocyte-poor platelet-rich plasma components have anti-inflammatory, angiogenetic, and radioprotective effects, reducing the activation of the NOX4, caspase and cytokine cascades, and inflammatory response severity through the MAPK/p38/JNK signaling pathway. This leads to the induction of endogenous antioxidant protection, the repair of post-radiation follicular damage, and slowing down the development of radiation-induced premature ovarian failure after electron irradiation.
Irisin Protects Musculoskeletal Homeostasis via a Mitochondrial Quality Control Mechanism
Authors: Zhao Chong , Wu Yonghao , Zhu Shuaiqi , Liu Haiying , Xu Shuai
Published: September 2024

Irisin, a myokine derived from fibronectin type III domain-containing 5 (FNDC5), is increasingly recognized for its protective role in musculoskeletal health through the modulation of mitochondrial quality control. This review synthesizes the current understanding of irisin’s impact on mitochondrial biogenesis, dynamics, and autophagy in skeletal muscle, elucidating its capacity to bolster muscle strength, endurance, and resilience against oxidative-stress-induced muscle atrophy. The multifunctional nature of irisin extends to bone metabolism, where it promotes osteoblast proliferation and differentiation, offering a potential intervention for osteoporosis and other musculoskeletal disorders. Mitochondrial quality control is vital for cellular metabolism, particularly in energy-demanding tissues. Irisin’s influence on this process is highlighted, suggesting its integral role in maintaining cellular homeostasis. The review also touches upon the regulatory mechanisms of irisin secretion, predominantly induced by exercise, and its systemic effects as an endocrine factor. While the therapeutic potential of irisin is promising, the need for standardized measurement techniques and further elucidation of its mechanisms in humans is acknowledged. The collective findings underscore the burgeoning interest in irisin as a keystone in musculoskeletal health and a candidate for future therapeutic strategies.
The Role of Propionate-Induced Rearrangement of Membrane Proteins in the Formation of the Virulent Phenotype of Crohn’s Disease-Associated Adherent-Invasive Escherichia coli
Authors: Pobeguts Olga V. , Galyamina Maria A. , Mikhalchik Elena V. , Kovalchuk Sergey I. , Smirnov Igor P. , Lee Alena V. , Filatova Lyubov Yu. , Sikamov Kirill V. , Panasenko Oleg M. , Gorbachev Alexey Yu.
Published: September 2024

Adhesive-invasive E. coli has been suggested to be associated with the development of Crohn’s disease (CD). It is assumed that they can provoke the onset of the inflammatory process as a result of the invasion of intestinal epithelial cells and then, due to survival inside macrophages and dendritic cells, stimulate chronic inflammation. In previous reports, we have shown that passage of the CD isolate ZvL2 on minimal medium M9 supplemented with sodium propionate (PA) as a carbon source stimulates and inhibits the adherent-invasive properties and the ability to survive in macrophages. This effect was reversible and not observed for the laboratory strain K12 MG1655. We were able to compare the isogenic strain AIEC in two phenotypes—virulent (ZvL2-PA) and non-virulent (ZvL2-GLU). Unlike ZvL2-GLU, ZvL2-PA activates the production of ROS and cytokines when interacting with neutrophils. The laboratory strain does not cause a similar effect. To activate neutrophils, bacterial opsonization is necessary. Differences in neutrophil NADH oxidase activation and ζ-potential for ZvL2-GLU and ZvL2-PA are associated with changes in membrane protein abundance, as demonstrated by differential 2D electrophoresis and LC-MS. The increase in ROS and cytokine production during the interaction of ZvL2-PA with neutrophils is associated with a rearrangement of the abundance of membrane proteins, which leads to the activation of Rcs and PhoP/Q signaling pathways and changes in the composition and/or modification of LPS. Certain isoforms of OmpA may play a role in the formation of the virulent phenotype of ZvL2-PA and participate in the activation of NADPH oxidase in neutrophils.
An Ingenane-Type Diterpene from Euphorbia kansui Promoted Cell Apoptosis and Macrophage Polarization via the Regulation of PKC Signaling Pathways
Authors: Feng Xiaoyi , Wang Lizhong , Pu Li , Li Jianchun , Li Hongmei , Liu Dan , Li Rongtao
Published: September 2024

Euphorbia kansui, a toxic Chinese medicine used for more than 2000 years, has the effect of “purging water to promote drinking” and “reducing swelling and dispersing modules”. Diterpenes and triterpenes are the main bioactive components of E. kansui. Among them, ingenane-type diterpenes have multiple biological activities as a protein kinase C δ (PKC-δ) activator, which have previously been shown to promote anti-proliferative and pro-apoptotic effects in several human cancer cell lines. However, the activation of PKC subsequently promoted the survival of macrophages. Recently, we found that 13-hydroxyingenol-3-(2,3-dimethylbutanoate)-13-dodecanoate (compound A) from E. kansui showed dual bioactivity, including the inhibition of tumor-cell-line proliferation and regulation of macrophage polarization. This study identifies the possible mechanism of compound A in regulating the polarization state of macrophages, by regulating PKC-δ-extracellular signal regulated kinases (ERK) signaling pathways to exert anti-tumor immunity effects in vitro, which might provide a new treatment method from the perspective of immune cell regulation.
Crocin Protects the 661W Murine Photoreceptor Cell Line against the Toxic Effects of All-Trans-Retinal
Authors: Yang Bo , Yang Kunhuan , Chen Jingmeng , Wu Yalin
Published: September 2024

Age-related macular degeneration (AMD) is a common disease contributing to vision loss in the elderly. All-trans-retinal (atRAL) is a retinoid in the retina, and its abnormal accumulation exhibits toxicity to the retina and promotes oxidative stress-induced photoreceptor degeneration, which plays a crucial role in AMD progression. Crocin is a natural product extracted from saffron, which displays significant antioxidant and anti-inflammatory effects. The present study elucidates the protective effects of crocin on photoreceptor cell damage by atRAL and its potential mechanisms. The results revealed that crocin significantly attenuated cytotoxicity by repressing oxidative stress, mitochondrial injury, and DNA damage in atRAL-loaded photoreceptor cells. Moreover, crocin visibly inhibited DNA damage-induced apoptosis and gasdermin E (GSDME)-mediated pyroptosis in photoreceptor cells after exposure to atRAL. It was also observed that crocin distinctly prevented an increase in Fe2+ levels and lipid peroxidation caused by atRAL via suppressing the Kelch-like ECH-associated protein 1 (KEAP1)/nuclear factor-erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) signaling pathway, thereby ameliorating photoreceptor cell ferroptosis. In short, these findings provide new insights that crocin mitigates atRAL-induced toxicity to photoreceptor cells by inhibiting oxidative stress, apoptosis, pyroptosis, and ferroptosis.
Role and Regulation of Glycogen Synthase Kinase-3 in Obesity-Associated Metabolic Perturbations
Authors: Lemon Jacob J. , Ogbu Comfort , Gupte Manisha
Published: September 2024

Obesity has become a global epidemic, contributing to various metabolic diseases. Despite existing therapies, the need to investigate new molecular targets to combat obesity-associated pathologies persists. Glycogen Synthase Kinase-3 (GSK-3), a serine/threonine kinase with two paralogs (GSK-3α and GSK-3β), has emerged as a critical player in obesity-associated metabolic pathologies such as type 2 diabetes (T2D), and cardiovascular diseases (CVDs). However, its ubiquitous dynamic expression and complex context-dependent signaling pathways present challenges in understanding its precise role in metabolic perturbations. In the present review, we will highlight the specific role and the proposed mechanisms via which the two GSK-3 paralogs impact obesity-associated pathologies such as T2D, diabetic cardiomyopathy (DCM), and cognitive impairment, a hallmark of Alzheimer’s disease (AD). We will also highlight studies delineating the role of GSK-3s using either GSK-3 inhibitors or non-pharmacological compounds to inhibit/taper GSK-3 activity in metabolic diseases. Thus, the primary goal of this review is to highlight recent findings delineating the regulation/dysregulation of GSK-3α/β in tissues such as heart, liver, skeletal muscle, pancreas, brain, and adipose tissue that undergo morphological and metabolic changes with diet-induced obesity which predisposes obese individuals to numerous devastating chronic conditions by GSK-3 overactivity.
SMURF1/2 Are Novel Regulators of WNK1 Stability
Authors: Jaykumar Ankita B. , Plumber Sakina , Binns Derk , Wichaidit Chonlarat , Luby-Phelps Katherine , Cobb Melanie H.
Published: September 2024

Angiogenesis is essential for remodeling and repairing existing vessels, and this process requires signaling pathways including those controlled by transforming growth factor beta (TGF-β). We have previously reported crosstalk between TGF-β and the protein kinase With No lysine (K) 1 (WNK1). Homozygous disruption of the gene encoding WNK1 results in lethality in mice near embryonic day E12 due to impaired angiogenesis, and this defect can be rescued by the endothelial-specific expression of an activated form of the WNK1 substrate kinase Oxidative Stress-Responsive 1 (OSR1). However, molecular processes regulated via a collaboration between TGF-β and WNK1/OSR1 are not well understood. Here, we show that WNK1 interacts with the E3 ubiquitin ligases SMURF1/2. In addition, we discovered that WNK1 regulates SMURF1/2 protein stability and vice versa. We also demonstrate that WNK1 activity regulates TGF-β receptor levels, in turn, controlling TGF-β signaling.
The Effect of Optimized Substrate Orientation on Layer Step in Laser Metal Deposition of Single-Crystal Nickel-Base Superalloys
Authors: Guo Jiachen , Zhou Junxiang , Sun Yong , Feng Bo , Zhang Yunwei , Ding Chang
Published: September 2024

Laser metal deposition is a promising way to repair the surface defects of single-crystal components in turbo engines. Understanding the mechanisms and improving the efficiency of the repair have been long-standing problems. In this study, the influence of the substrate orientation on the laser metal deposition (LMD) was investigated and its effect on repair layer-step was examined. LMD experiments were conducted on single crystal superalloys with a normal substrate orientation (001)/[100] and with an optimized substrate orientation (101)/[101¯]. It reveals that the laser cladding with the optimized orientation leads to a larger height of the [001] dendrite region than that with the normal orientation. The calculated results of the growth velocity, thermal gradient, and susceptibility to CET in the dendrite-preferred growth direction indicate that, for the (101)/[101¯] orientation, the [001]/[100] boundary is located at relative high position in each layer, which not only decreases the formation ability of stray grain significantly, but also eliminates the appearance of the maximum susceptibility. This makes the necessary dilution position much higher, and thus, a large cladding step can be selected. Our findings could find potential applications in laser repair of single-crystal components.
Thermophysical Properties’ Enhancement of LiNO3-NaNO3-KNO3-NaNO2-KNO2 Mixed with SiO2/MgO Nanoparticles
Authors: Zhu Chuang , Xu Minhao , Tian Baiyuan , Gu Manting , Gong Li
Published: September 2024

The aim of this study is to further enhance the thermal storage and heat transfer performances of a low-melting-point quinary salt. The eutectic salt was prepared using LiNO3, NaNO3, KNO3, NaNO2, and KNO2 as raw materials, followed by the doping of nano-SiO2 and nano-MgO into the base salt using a microwave-assisted method. The thermal properties of the samples were analyzed using a Synchronous Thermal Analyzer and a Laser Flash Apparatus. The co-doping of two types of nanoparticles was found to significantly enhance the specific heat capacity of the base salt. The maximum specific heat reached 2.36 J/(g·K), showing a 50.4% increase compared to the base salt. The thermal conductivity of molten salts can be affected by nanoparticles. An observed sample demonstrated a thermal diffusivity of 0.286 mm2/s, indicating a 19.2% improvement over the base salt, which may be attributed to enhanced phonon thermal efficiency. In addition, this study revealed that while interfacial thermal resistance can enhance specific heat capacity, it can also lead to a decrease in the thermal conductivity efficiency of materials. This work can offer insights and references for the enhancement of molten salt properties.
Analysis of Wall Thickness and Absorption Characteristics of Ammonium Nitrate(V) from Various Sources
Authors: Biessikirski Andrzej , Kaczmarczyk Grzegorz Piotr , Kuterasiński Łukasz , Machowski Grzegorz , Stopkowicz Agnieszka , Ruggiero-Mikołajczyk Małgorzata
Published: September 2024

This study investigates the wall thickness and specific surface area (SBET) of ammonium nitrate(V) samples of varying provenance. The research focuses on both fertilizer-grade ammonium nitrate(V) and three porous prill samples obtained from different manufacturers. The samples were analyzed using tomography scanning and two distinct porosimetry methods. The wall thickness analysis revealed that fertilizer-grade ammonium nitrate(V) possesses thicker walls, ranging from 0.05 to 0.40 mm, compared to porous prill-type ammonium nitrate(V), which predominantly exhibited wall thicknesses between 0.05 and 0.025 mm, with occasional thicker regions up to 0.040 mm. These variations in wall thickness are likely attributable to differences in manufacturing processes and prilling conditions specific to the ammonium nitrate(V) porous prill-type samples. The specific surface area (SBET), derived from nitrogen adsorption measurements, indicated that the samples exhibited surface areas ranging from 0.011 to 0.466 m2·g, suggesting that these samples do not exhibit particularly high absorption capacities. However, the SBET values obtained from the mercury intrusion method suggested significantly higher absorption capacities, falling within the range of 4.87–18.29 m2·g. These findings suggest that mercury porosimetry may provide a more accurate assessment of the porosity and absorption potential of ammonium nitrate(V) samples.
Energy-Efficient and Effective MCF-7 Cell Ablation and Electrothermal Therapy Enabled by M13–WS2–PEG Nanostructures
Authors: Meivita Maria P. , Mozar Fitya S. , Go Shao-Xiang , Li Lunna , Bajalovic Natasa , Loke Desmond K.
Published: September 2024

Thermal agents (TAs) have exhibited promise in clinical tests when utilized in cancer thermal therapy (TT). While rapid degradation of TAs may address safety concerns, it limits the thermal stability required for effective treatment. TAs, which possess exceptional thermal stability, experience gradual deterioration. There are few approaches that effectively address the trade-off between improving thermal stability and simultaneously boosting material deterioration. Here, we control the thermal character of tungsten disulfide (WS2)-based 2D materials by utilizing an M13 phage through Joule heating (the M13–WS2–PEG nanostructures were generated and termed a tripartite (T) nanostructure), and developed a T nanostructure-driven TT platform (we called it T-TT) for efficient thermal ablation of clinically relevant MCF-7 cells. A relative cell viability of ~59% was achieved, as well as onset time of degradation of ~0.5 week. The T-TT platform also discloses an energy density of 5.9 J/mL. Furthermore, the phage-conjugated WS2 can be utilized to achieve ultrasound imaging for disease monitoring. Therefore, this research not only presents a thermal agent that overcomes TA limitations, but also demonstrates a practical application of WS2-type material system in ultra-energy efficient and effective cancer therapy.
Study on the Rheological Behaviors, Thixotropy, and Printing Characteristics of Screen Printing Slurry for Nd-Fe-B
Authors: Sun Xiaojun , Lin Xiao , Luo Yang , Yu Dunbo , Yan Wenlong , Zhang Hongbin , Wang Zilong , Zhang Chaofan , Guo Jiyuan , Zhang Wendi , Gao Weiguo , Huang Shan
Published: September 2024

The rheological behavior and printing characteristics of the screen-printing slurry for Nd-Fe-B grain boundary diffusion are key factors that determine the quality of printing and magnetic performance. However, few studies have focused on the organic medium, a crucial material for slurry. In this paper, the rheology, thixotropy, and thermal decomposition behavior of the organic vehicle in Nd-Fe-B screen printing slurry were studied. The results show that the organic vehicle formed by terpineol and polyvinyl butyral (PVB) exhibits typical non-Newtonian fluid characteristics, with excellent rheology and thixotropy, ensuring that the slurry prepared from it has excellent static stability and printing consistency. Additionally, the carbon residue of the organic vehicle formed by terpineol and PVB is less than 0.1% at 900 °C, avoiding excessive carbon entering the magnet during the diffusion process. Moreover, studying the rheology and thixotropy of the organic vehicle through a rheometer can quickly screen the slurry system. This work provides valuable guidance for designing an organic vehicle for screen-printing slurry for Nd-Fe-B grain boundary diffusion in future research.
Research on Coal-Releasing Characteristics of Hydraulic Support for a Large Inclined-Angle Comprehensive Workface
Authors: Cao Lianmin , Geng Mingyan , Shen Rui , Zhang Dekang , Zhang Xiaowei
Published: September 2024

This research aimed to examine shortcomings such as low efficiency and unstable coal release of 2313 large inclined-angle comprehensively released working faces in 230 mining areas of a mine in Shandong. Through the study of the actual working conditions of the large inclined-angle comprehensive release working face, it is concluded that the mining efficiency of the large inclined-angle coal seam is mainly related to the sequence of coal release, the step distance of the coal release, the distance between the end faces, the mining height, the working resistance of the hydraulic bracket and other factors. Taking ZF5600/16.5/26 hydraulic support as an example, simulation software was used to study the influencing factors of coal release efficiency, and the most efficient coal release method was found through the control variable method, so as to improve the mining recovery efficiency of the overall working face of the large inclined-angle coal seam, and after optimizing the working conditions of the hydraulic support, the top coal recovery efficiency was increased by 14.3% compared with the previous one after the actual statistics of the field situation.
Automated High-Speed Approaches for the Extraction of Permanent Magnets from Hard-Disk Drive Components for the Circular Economy
Authors: Burkhardt Carlo , Ortiz Francisco , Daoud Kaies , Björnfot Tomas , Ahrentorp Fredrik , Blomgren Jakob , Walton Allan
Published: September 2024

This work describes an automated pilot plant for the extraction of rare-earth (RE) permanent magnets from computer hard-disk drives (HDDs), demonstrating a commercially viable way to exploit these abundant sources of end-of-life (EOL) magnets. A mobile approach is provided for the on-site destruction of the HDDs in server farms, in compliance with the European Data Protection Regulation (GDPR), enabling both separation of the magnets and automated shredding of the data carrier. This fully automated process identifies (both optically and magnetically) the location of the rare-earth magnets and cuts off the corner of the hard drive containing the rare-earth material in the voice coil motor. This allows for a significant reduction in magnet extraction time (6 s per HDD) compared to previously reported semi-automated (2 min) and manual (5 min) dismantling times. This work will also help to transfer the experience gained in the mobile pilot plant to other future sources of EOL materials such as drive motors and mixed electronic scrap.
Sanger Sequencing Reveals Novel Variants in GLO-1, ACE, and CBR1 Genes in Patients of Early and Severe Diabetic Nephropathy
Authors: Shah Syed Zubair Hussain , Rashid Amir , Majeed Asifa , Ghafoor Tariq , Azam Nadeem
Published: September 2024

Background and Objectives: Diabetes is a global health issue, with approximately 50% of patients developing diabetic nephropathy (DN) and 25% experiencing early and severe forms of the disease. The genetic factors contributing to rapid disease progression in a subset of these patients are unclear. This study investigates genetic variations in the GLO-1, CBR-1, and ACE genes associated with early and severe DN. Materials and Methods: Sanger DNA sequencing of the exons of CBR1, GLO1, and ACE genes was conducted in 113 patients with early and severe DN (defined as occurring within 10 years of the diagnosis of diabetes and with eGFR < 45 mL/min/1.73 m2) and 100 controls. The impact of identified genetic variations was analyzed using computational protein models created in silico with SWISS-Model and SWISS-Dock for ligand binding interactions. Results: In GLO1, two heterozygous missense mutations, c.102G>T and c.147C>G, and one heterozygous nonsense mutation, c.148G>T, were identified in patients. The SNP rs1049346 (G>A) at location 6:38703061 (GRCh38) was clinically significant. The c.147C>G mutation (C19S) was associated with ligand binding disruption in the GLO1 protein, while the nonsense mutation resulted in a truncated, non-functional protein. In CBR1, two heterozygous variations, one missense c.358G>A, and one silent mutation c.311G>C were observed, with the former (D120N) affecting the active site. No significant changes were noted in ACE gene variants concerning protein structure or function. Conclusions: The study identifies four novel and five recurrent mutations/polymorphisms in GLO1, ACE, and CBR1 genes associated with severe DN in Pakistani patients. Notably, a nonsense mutation in GLO1 led to a truncated, non-functional protein, while missense mutations in GLO1 and CBR1 potentially disrupt enzyme function, possibly accelerating DN progression.
Cardiovascular Effectiveness and Safety of Antidiabetic Drugs in Patients with Type 2 Diabetes and Peripheral Artery Disease: Systematic Review
Authors: Cimellaro Antonio , Cavallo Michela , Mungo Marialaura , Suraci Edoardo , Spagnolo Francesco , Addesi Desirée , Pintaudi Medea , Pintaudi Carmelo
Published: September 2024

Peripheral artery disease (PAD) is an atherosclerotic condition commonly complicating type 2 diabetes (T2D), leading to poor quality of life and increased risk of major adverse lower-limb (MALE) and cardiovascular (CV) events (MACE). Therapeutic management of PAD in T2D patients is much more arduous, often due to bilateral, multi-vessel, and distal vascular involvement, in addition to increased systemic polyvascular atherosclerotic burden. On the other hand, the pathophysiological link between PAD and T2D is very complex, involving mechanisms such as endothelial dysfunction and increased subclinical inflammation in addition to chronic hyperglycemia. Therefore, the clinical approach should not ignore vascular protection with the aim of reducing limb and overall CV events besides a mere glucose-lowering effect. However, the choice of the best medications in this setting is challenging due to low-grade evidence or lacking targeted studies in PAD patients. The present review highlighted the strong relationship between T2D and PAD, focusing on the best treatment strategy to reduce CV risk and prevent PAD occurrence and worsening in patients with T2D. The Medline databases were searched for studies including T2D and PAD up to June 2024 and reporting the CV effectiveness and safety of the most used glucose-lowering agents, with no restriction on PAD definition, study design, or country. The main outcomes considered were MACE—including nonfatal acute myocardial infarction, nonfatal stroke, and CV death—and MALE—defined as lower-limb complications, amputations, or need for revascularization. To the best of our current knowledge, GLP-1 receptor agonists and SGLT2 inhibitors represent the best choice to reduce CV risk in T2D and PAD settings, but a personalized approach should be considered. GLP-1 receptor agonists should be preferred in subjects with prevalent atherosclerotic burden and a history of previous MALE, while SGLT2 inhibitors should be used in those with heart failure if overall CV benefits outweigh the risk of lower-limb complications.
Adsorptive Stripping Voltammetric Quercetin Determination in Pharmaceuticals and Urine Samples Using a Long Service-Life Array of Carbon Composite Microelectrodes
Authors: Gęca Iwona , Korolczuk Mieczyslaw
Published: September 2024

This article presents for the first time a new working electrode with a long service life— the bismuth-plated array of carbon composite microelectrodes for the simple, fast and sensitive determination of quercetin by adsorptive stripping voltammetry. The main experimental conditions were selected. The calibration graph was linear from 1 × 10−9 to 2 × 10−8 mol L−1 with an accumulation time of 60 s. The detection limit was equal to 4.8 × 10−10 mol L−1. The relative standard deviation for 2 × 10−8 mol L−1 of quercetin was 4.4% (n = 7). Possible interference effects resulting from the presence of other organic and surface active compounds and interfering ions were studied. The developed procedure was successfully applied to determine quercetin in pharmaceutical preparations and the spiked urine samples.
Green Tea Kombucha Impacts Inflammation and Salivary Microbiota in Individuals with Excess Body Weight: A Randomized Controlled Trial
Authors: Fraiz Gabriela Macedo , Bonifácio Dandara Baia , Lacerda Udielle Vermelho , Cardoso Rodrigo Rezende , Corich Viviana , Giacomini Alessio , Martino Hércia Stampini Duarte , Echeverría Sergio Esteban , Barros Frederico Augusto Ribeiro de , Milagro Fermín I. , Bressan Josefina
Published: September 2024

Background: Green tea kombucha (GTK) is a fermented beverage with promising health benefits, but few studies proved its impact on human health. Thus, we aimed to investigate the impact of GTK on weight loss, inflammation, and salivary microbiota in individuals with excess body weight. Methods: This is a randomized controlled clinical trial that lasted 10 weeks with two groups of individuals with excess body weight: control (CG; n = 29; caloric restriction) and kombucha (KG; n = 30; caloric restriction + 200 mL GTK). Body composition, anthropometry, saliva, and blood collection were performed in the beginning and end of the intervention. Plasma interleukins were determined by flow cytometry. Salivary microbiota was analyzed by 16S rRNA sequencing. Results: Both groups decreased weight, BMI, and body fat (p < 0.001) after the intervention, but there were no differences between groups. The KG reduced lipid accumulation product (LAP) (p = 0.029). Both groups decreased IL-1β and IL-8, but IL-6 increased in the CG (p = 0.023) compared to the kombucha group. Alpha and beta diversity of salivary microbiota increased in the KG. Moreover, the KG presented lower Bacillota/Bacteroidota ratio (p = 0.028), and BMI was positively associated with the Bacillota phylum. Conclusions: GTK did not enhance weight loss, but it decreased the LAP. GTK helped in the inflammatory profile and induced positive changes in oral microbiota composition.
Study of Potential Blocking Peptides Targeting the SARS-CoV-2 RBD/hACE2 Interaction
Authors: Villada-Troncoso Sara M. , Arévalo-Romero Jenny Andrea , Hernández Rivera Vanessa , Pedraza-Escalona Martha , Pérez-Tapia Sonia M. , Espejo-Mojica Angela Johana , Alméciga-Díaz Carlos Javier
Published: September 2024

Background/Objectives: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, was declared a public health emergency in early 2020. The infection initiates when the receptor-binding domain (RBD) of the viral spike protein binds to human angiotensin-converting enzyme 2 (ACE2). Despite the success of vaccination efforts, the emergence of new variants highlights the ongoing need for treatments targeting these evolving strains. In silico methods previously identified peptides BP2, BP9, and BP11 as being capable of disrupting the RBD-ACE2 interaction, though their efficacy has not been experimentally validated until now. Methods: In this study, these peptides were recombinantly produced in the yeast Komagataella phaffii, and the activity was assessed in vitro using binding assays with multiple RBD variants and the inhibition of the RBD-ACE2 interaction. Results: The production yield for BP2, BP9, and BP11 was 14.34, 4.01, and 1.35 mg per culture liter, respectively. Noteworthy, the three BPs interacted with the RBD of SARS-CoV-2 variants of concern, with BP2 showing higher recognition. Finally, the BPs showed an RBD/hACE2 interaction blocking capacity with IC50 values between 1.03 and 5.35 nM, with BP2 showing the lowest values among the evaluated peptides. Conclusions: These results demonstrate that BP2, specifically, is a promising candidate for the development of novel therapeutic interventions targeting SARS-CoV-2 and other coronaviruses that use hACE2 for cellular entry.
From Subjects to Assemblages: Insights from Oldboy
Authors: Lazić Gordana
Published: September 2024

Drawing on the insights of media ecology, this essay explores the potential of media to mobilize representations, feelings, and habits to transform individuals into extensions of media themselves. Specifically, I undertake an analysis of the South Korean film Oldboy, which I argue demonstrates how, in the contemporary moment, media narratively and affectively mobilize individuals to become not only ideological subjects but also media appendages that, consequently, carry out cinema’s central functions.
Brassica rapa BrICE1 and BrICE2 Positively Regulate the Cold Tolerance via CBF and ROS Pathways, Balancing Growth and Defense in Transgenic Arabidopsis
Authors: Wu Wangze , Yang Haobo , Xing Peng , Zhu Guoting , Han Xueyan , Xue Mei , Min Guotai , Ding Haijun , Wu Guofan , Liu Zigang
Published: September 2024

Winter rapeseed (Brassica rapa) has a good chilling and freezing tolerance. inducer of CBF expression 1 (ICE1) plays a crucial role in cold signaling in plants; however, its role in Brassica rapa remains unclear. In this study, we identified 41 ICE1 homologous genes from six widely cultivated Brassica species. These genes exhibited high conservation, with evolutionary complexity between diploid and allotetraploid species. Cold stress induced ICE1 homolog expression, with differences between strongly and weakly cold-tolerant varieties. Two novel ICE1 paralogs, BrICE1 and BrICE2, were cloned from Brassica rapa Longyou 6. Subcellular localization assays showed that they localized to the nucleus, and low temperature did not affect their nuclear localization. The overexpression of BrICE1 and BrICE2 increased cold tolerance in transgenic Arabidopsis and enhanced reactive oxygen species’ (ROS) scavenging ability. Furthermore, our data demonstrate that overexpression of BrICE1 and BrICE2 inhibited root growth in Arabidopsis, and low temperatures could induce the degradation of BrICE1 and BrICE2 via the 26S-proteasome pathway. In summary, ICE1 homologous genes exhibit complex evolutionary relationships in Brassica species and are involved in the C-repeat/DREB binding factor (CBF) pathway and ROS scavenging mechanism in response to cold stress; these regulating mechanisms might also be responsible for balancing the development and cold defense of Brassica rapa.
A Probabilistic Statistical Risk Assessment Method for Soil Erosion Using Remote Sensing Data: A Case Study of the Dali River Basin
Authors: Zhao Hao , Cheng Yuhui , Zhang Xiwang , Yu Shiqi , Chen Mengwei , Zhang Chengqiang
Published: September 2024

Soil erosion risk assessment enables the identification of areas requiring priority treatment and avoids wasting human and material resources. The factor scoring method used in existing studies has high subjectivity, and the method of expressing erosion risk according to the soil erosion intensity ignores the random nature of the occurrence of erosion; therefore, neither method accurately reflects the risk of soil erosion. In order to address this issue, this study proposes a soil erosion risk assessment method that integrates the outcome and the probability of occurrence of soil erosion by means of a probabilistic statistical model. Subsequently, experimental research is conducted in the Dali River Basin. On the basis of long time-series data, using mathematical statistics as a tool and drawing on the empirical frequency formula, the probabilistic statistical risk assessment model is combined with the Modified Universal Soil Loss Equation (RUSLE) model to account for the probability of regional soil erosion at different intensity levels in the long time-series, which is combined with the intensity of erosion to carry out soil erosion risk assessment. The results of our study show the following: (1) The central and southwestern regions of the Dali River Basin (DRB) present medium and high levels of soil erosion risk, with the proportion of low-risk areas increasing annually, accounting for 78.97% of the DRB in 2020, while extremely high-risk areas account for only 0.40% of the DRB. (2) The major components impacting soil erosion risk in the DRB, as revealed by the geodetector, are the normalized difference vegetation index (NDVI) and slope, where the interaction between the two dominated the spatial variation in soil erosion risk. (3) Comparing the soil erosion risk and its status in the coming years, the proposed assessment method based on the occurrence probability can reveal the future soil erosion risk better than the traditional assessment method.
A Complex Background SAR Ship Target Detection Method Based on Fusion Tensor and Cross-Domain Adversarial Learning
Authors: Chan Haopeng , Qiu Xiaolan , Gao Xin , Lu Dongdong
Published: September 2024

Synthetic Aperture Radar (SAR) ship target detection has been extensively researched. However, most methods use the same dataset division for both training and validation. In practical applications, it is often necessary to quickly adapt to new loads, new modes, and new data to detect targets effectively. This presents a cross-domain detection problem that requires further study. This paper proposes a method for detecting SAR ships in complex backgrounds using fusion tensor and cross-domain adversarial learning. The method is designed to address the cross-domain detection problem of SAR ships with large differences between the training and test sets. Specifically, it can be used for the cross-domain detection task from the fully polarised medium-resolution ship dataset (source domain) to the high-resolution single-polarised dataset (target domain). This method proposes a channel fusion module (CFM) based on the YOLOV5s model. The CFM utilises the correlation between polarised channel images during training to enrich the feature information of single-polarised images extracted by the model during inference. This article proposes a module called the cross-domain adversarial learning module (CALM) to reduce overfitting and achieve adaptation between domains. Additionally, this paper introduces the anti-interference head (AIH) which decouples the detection head to reduce the conflict of classification and localisation problems. This improves the anti-interference and generalisation ability in complex backgrounds. This paper conducts cross-domain experiments using the constructed medium-resolution SAR full polarisation dataset (SFPD) as the source domain and the high-resolution single-polarised ship detection dataset (HRSID) as the target domain. Compared to the best-performing YOLOV8s model among typical mainstream models, this model improves precision by 4.9%, recall by 3.3%, AP by 2.4%, and F1 by 3.9%. This verifies the effectiveness of the method and provides a useful reference for improving cross-domain learning and model generalisation capability in the field of target detection.
Variation in Vegetation Composition and Structure across Mudflat Areas in the Yellow River Delta, China
Authors: Li He , Liu Qingsheng , Huang Chong , Zhang Xin , Wang Shuxuan , Wu Wei , Shi Lei
Published: September 2024

Variations in vegetation composition and structure are significant signals of the succession of mudflat ecosystems and have important indicative roles in mudflat ecosystem degradation. Due to poor accessibility of vast even mudflat areas, variation in vegetation composition and structure across mudflat areas remains unclear in the Yellow River Delta (YRD), China. We provided an UAV multispectral orthomosaic with 10 cm ground sample distance to classify and compare the vegetation composition and structure across mudflat areas in the YRD. The vegetation classification overall accuracy achieved 95.0%. We found that although a significant difference (p < 0.05) was checked out in terms of the Shannon–Wiener diversity index (from 1.33 to 0.92) and evenness index (from 0.96 to 0.66) among the eight subareas from land to sea, all four dominant vegetation communities (S. salsa, L. bicolor, T. chinensis, and P. australis) were discovered at all eight subareas. Our findings support the idea that the regional environment and local microtopography are the predominant forces for variation in vegetation composition and structure across mudflat areas. From the perspective of vegetation restoration and conservation, changing the local microtopography will be an interesting way to enhance the vegetation diversity of the mudflat ecosystems in the YRD.
Cross-Country Ski Skating Style Sub-Technique Detection and Skiing Characteristic Analysis on Snow Using High-Precision GNSS
Authors: Uda Shunya , Miyamoto Naoto , Hirose Kiyoshi , Nakano Hiroshi , Stöggl Thomas , Linnamo Vesa , Lindinger Stefan , Takeda Masaki
Published: September 2024

A comprehensive analysis of cross-country skiing races is a pivotal step in establishing effective training objectives and tactical strategies. This study aimed to develop a method of classifying sub-techniques and analyzing skiing characteristics during cross-country skiing skating style timed races on snow using high-precision kinematic GNSS devices. The study involved attaching GNSS devices to the heads of two athletes during skating style timed races on cross-country ski courses. These devices provided precise positional data and recorded vertical and horizontal head movements and velocity over ground (VOG). Based on these data, sub-techniques were classified by defining waveform patterns for G2, G3, G4, and G6P (G6 with poling action). The validity of the classification was verified by comparing the GNSS data with video analysis, a process that yielded classification accuracies ranging from 95.0% to 98.8% for G2, G3, G4, and G6P. Notably, G4 emerged as the fastest technique, with sub-technique selection varying among skiers and being influenced by skiing velocity and course inclination. The study’s findings have practical implications for athletes and coaches as they demonstrate that high-precision kinematic GNSS devices can accurately classify sub-techniques and detect skiing characteristics during skating style cross-country skiing races, thereby providing valuable insights for training and strategy development.
High-Voltage Power Supply for Four-Quadrant Dielectric Elastomer Actuators
Authors: Xing Haoyue , Hao Qun , Yao Cancan , Zhang Zitong , Li Jiafu , Cheng Yang
Published: September 2024

Dielectric elastomer actuators (DEAs) are emerging as promising candidates for various applications in robotics and optical devices due to their lightweight, miniaturization potential, high energy density, simple structure, and low power consumption. However, their effective actuation always demands sophisticated high-voltage driving circuits that are compact and responsive. DEAs need to be capable of generating intricate high-voltage waveforms or simultaneously controlling multiple quadrants with distinct high-voltage levels. This paper proposes a high-voltage power supply for DEAs, featuring a four-quadrant high-voltage driving circuit. The circuit is capable of independently generating high-voltage signals ranging from 100 V to 6000 V and producing arbitrary waveforms with adjustable frequencies. The independent operation of the quadrants without crosstalk showcases the system’s integration and potential for cross-disciplinary applications.
Analysis of Field Trial Results for Excavation-Activities Monitoring with φ-OTDR
Authors: Zhang Hailiang , Dong Hui , Hu Dora Juan Juan , Vuong Nhu Khue , Jiang Lianlian , Lim Gen Liang , Ng Jun Hong
Published: September 2024

Underground telecommunication cables are highly susceptible to damage from excavation activities. Preventing accidental damage to underground telecommunication cables is critical and necessary. In this study, we present field trial results of monitoring excavation activities near underground fiber cables using an intensity-based phase-sensitive optical time-domain reflectometer (φ-OTDR). The reasons for choosing intensity-based φ-OTDR for excavation monitoring are presented and analyzed. The vibration signals generated by four typical individual excavation events, i.e., cutting, hammering, digging, and tamping at five different field trial sites, as well as five different mixed events in the fifth field trial site were investigated. The findings indicate that various types of events can generate vibration signals with different features. Typically, fundamental peak frequencies of cutting, hammering and tamping events ranged from 30 to 40 Hz, 11 to 15 Hz, and 30 to 40 Hz, respectively. Digging events, on the other hand, presented a broadband frequency spectrum without a distinct peak frequency. Moreover, due to differences in environmental conditions, even identical excavation events conducted with the same machine may also generate vibration signals with different characteristics. The diverse field trial results presented offer valuable insights for both research and the practical implementation of excavation monitoring techniques for underground cables.
Active Region Mode Control for High-Power, Low-Linewidth Broadened Semiconductor Optical Amplifiers for Light Detection and Ranging
Authors: Tang Hui , Zhang Meng , Liang Lei , Zhang Tianyi , Qin Li , Song Yue , Lei Yuxin , Jia Peng , Wang Yubing , Qiu Cheng , Zheng Chuantao , Li Xin , Chen Yongyi , Li Dan , Ning Yongqiang , Wang Lijun
Published: September 2024

This paper introduces a semiconductor optical amplifier (SOA) with high power and narrow linewidth broadening achieved through active region mode control. By integrating mode control with broad-spectrum epitaxial material design, the device achieves high gain, high power, and wide band output. At a wavelength of 1550 nm and an ambient temperature of 20 °C, the output power reaches 757 mW when the input power is 25 mW, and the gain is 21.92 dB when the input power is 4 mW. The 3 dB gain bandwidth is 88 nm, and the linewidth expansion of the input laser after amplification through the SOA is only 1.031 times. The device strikes a balance between high gain and high power, offering a new amplifier option for long-range light detection and ranging (LiDAR).