bluuebunny/crossref_metadata_2025 huggingface dataset:
Future Reductions in Suitable Habitat for Key Tree Species Result in Declining Boreal Forest Aboveground Biomass Carbon in China
Authors: Zhu Bin , Zhang Zengxin , Kong Rui , Wang Meiquan , Li Guangshuai , Sui Xiran , Tao Hui
Published: October 2023

China’s forest ecosystem plays a crucial role in carbon sequestration, serving as a cornerstone in China’s journey toward achieving carbon neutrality by 2060. Yet, previous research primarily emphasized climate change’s influence on forest carbon sequestration, neglecting tree species’ suitable area changes. This study combinates the Lund–Potsdam–Jena model (LPJ) and the maximum entropy model (MaxENT) to reveal the coupling impacts of climate and tree species’ suitable area changes on forest aboveground biomass carbon (ABC) in China. Key findings include the following: (1) China’s forests are distributed unevenly, with the northeastern (temperate coniferous broad-leaved mixed forest, TCBMF), southwestern, and southeastern regions (subtropical evergreen broad-leaved forest, SEBF) as primary hubs. Notably, forest ABC rates in TCBMF exhibited a worrisome decline, whereas those in SEBF showed an increasing trend from 1993 to 2012 based on satellite observation and LPJ simulation. (2) Under different future scenarios, the forest ABC in TCBMF is projected to decline steadily from 2015 to 2060, with the SSP5-8.5 scenario recording the greatest decline (−4.6 Mg/ha/10a). Conversely, the forest ABC in SEBF is expected to increase under all scenarios (2015–2060), peaking at 1.3 Mg/ha/10a in SSP5-8.5. (3) Changes in forest ABC are highly attributed to climate and changes in tree species’ highly suitable area. By 2060, the suitable area for Larix gmelinii in TCBMF will significantly reduce to a peak of 65.71 × 104 km2 under SSP5-8.5, while Schima superba Gardner & Champ and Camphora officinarum in SEBF will expand to peaks of 94.07 × 104 km2 and 104.22 × 104 km2, respectively. The geographic detector’s results indicated that the climate and tree species’ suitable area changes showed bi-variate and nonlinear enhanced effects on forest ABC change. These findings would offer effective strategies for achieving carbon neutrality.
Fuel Type Mapping Using a CNN-Based Remote Sensing Approach: A Case Study in Sardinia
Authors: Carbone Andrea , Spiller Dario , Laneve Giovanni
Published: October 2023

Accurate fuel mapping is crucial for effectively determining wildfire risk and implementing management strategies. The primary challenge in fuel type mapping lies in the need to develop accurate and efficient methods for identifying and categorizing the various combustible materials present in an area, often on a large scale. In response to this need, this paper presents a comprehensive approach that combines remote sensing data and Convolutional Neural Network (CNN) to discriminate between fire behavior fuel models. In particular, a CNN-based classification approach that leverages Sentinel-2 imagery is exploited to accurately classify fuel types into seven preliminary main classes (broadleaf, conifers, shrubs, grass, bare soil, urban areas, and water bodies). To further refine the fuel mapping results, subclasses were generated from the seven principles by using biomass and bioclimatic maps. These additional maps provide complementary information about vegetation density and climatic conditions, respectively. By incorporating this information, we align our fuel type classification with the widely used Standard Scott and Burgan (2005) fuel classification system. The results are highly promising, showcasing excellent CNN training performance with all three metrics—accuracy, recall, and F1 score—achieving an impressive 0.99%. Notably, the network exhibits exceptional accuracy in a test case conducted in the southern region of Sardinia, successfully identifying Burnable classes in previously unseen pixels: broadleaf at 0.99%, conifer at 0.79%, shrub at 0.76%, and grass at 0.84%. The proposed approach presents a valuable tool for enhancing fire management, contributing to more effective wildfire prevention and mitigation efforts. Thus, this tool could be leveraged by fire management agencies, policymakers, and researchers to improve the determination of wildfire risk and management.
Quantitative Textural and Rheological Data on Different Levels of Texture-Modified Food and Thickened Liquids Classified Using the International Dysphagia Diet Standardisation Initiative (IDDSI) Guideline
Authors: Wong Man Chun , Chan Karen M. K. , Wong Tsz Ting , Tang Ho Wah , Chung Hau Yin , Kwan Hoi Shan
Published: October 2023

Diet modification is a common compensation strategy to promote swallowing safety in patients with swallowing difficulties. The International Dysphagia Diet Standardisation Initiative (IDDSI) guideline provides qualitative descriptions on texture-modified food and thickened liquid. This study aimed to establish quantitative textural and rheological data on different IDDSI levels based on common Chinese ingredients and dishes. Textural and rheological properties of 226 samples of various food textures and 93 samples of various liquid consistencies were obtained using a texture profile analysis (TPA) and viscometer, respectively. The establishment of such quantitative data can be used for future texture-modified food product development and research purposes.
Overview of the Sustainable Valorization of Using Waste and By-Products in Grain Processing
Authors: Danciu Cristina-Anca , Tulbure Anca , Stanciu Mirela-Aurora , Antonie Iuliana , Capatana Ciprian , Zerbeș Mihai Victor , Giurea Ramona , Rada Elena Cristina
Published: October 2023

In an increasingly resource-constrained era, using waste and by-products from grain processing has a wide appeal. This is due to the nutritive value and economic aspects of this process and due to its compatibility with the trend towards more sustainable food systems. Following the fundamentals of circular economy, a current need is the effective utilization of grain waste and by-products for conversion into value-added products in the food industry. The aim of this study is twofold: (1) using bibliometrics and the literature found in various databases, we aim to understand the progress of valorizing grain waste and by-products in human nutrition. The literature within various databases, namely, Google Scholar, Web of Science, and Elsevier Scopus, has been evaluated for its merits and values. (2) We aim to explore knowledge-based strategies by reviewing the literature concerning the possible use of grain waste and by-products for the food processing industry, reducing the burden on virgin raw materials. The review allowed us to unlock the latest advances in upcycling side streams and waste from the grain processing industry.
Operator Kernel Functions in Operational Calculus and Applications in Fractals with Fractional Operators
Authors: Yu Xiaobin , Yin Yajun
Published: October 2023

In this study, we delve into the general theory of operator kernel functions (OKFs) in operational calculus (OC). We established the rigorous mapping relation between the kernel function and the corresponding operator through the primary translation operator e−pt, which bears a striking resemblance to the Laplace transform. Our research demonstrates the uniqueness of the kernel function, determined by the rules of operational calculus and its integral representation. This discovery provides a novel perspective on how the operational calculus can be understood and applied, particularly through convolution with kernel functions. We substantiate the accuracy of the proposed method by demonstrating the consistency between the operator solution and the classical solution for the heat conduction problem. Subsequently, on the fractal tree, fractal loop, and fractal ladder structures, we illustrate the application of operational calculus in viscoelastic constitutive and hemodynamics confirming that the method proposed unifies the OKFs in the existing OC theory and can be extended to the operator field. These results underscore the practical significance of our results and open up new possibilities for future research.
Investigating Runway Incursion Incidents at United States Airports
Authors: Omosebi Olajumoke , Azimi Mehdi , Olowokere David , Wanyan Yachi , Zhao Qun , Qi Yi
Published: October 2023

According to the Federal Aviation Administration (FAA), the number of runway incursions is increasing. Over the last two decades, the number of runway incursions at U.S. airports has increased from 987 in 2002 to 25,036 in 2020. Runway incursions are a major threat to aviation safety, causing major delays and financial consequences for airlines, as well as injury or death through incidents such as aircraft collisions. The FAA promotes the implementation of runway safety technology, infrastructure, procedural methods, alterations to airport layouts, and training practices to reduce the frequency of runway incursions. In this paper, the relationship between airport geometry factors, mitigating technologies, and the number of runway incursions at large hub airports in the United States was investigated using a random effects Poisson model for analyses of panel data. Airport operations data from the FAA Air Traffic Activity System, runway incursion data from the FAA Aviation Safety Information Analysis and Sharing System from 2002 to 2020, and airport geometry data created using airport geometry features from the FAA airport diagrams were collected. Thirty large hub airports with FAA-installed mitigating technologies were investigated. The model identified significant variables that correlate with runway incursions for large hub airport categories defined by the National Plan of Integrated Airport Systems (NPIAS). The model results indicate that airports with significant numbers of runway-to-runway intersection points increase runway incursion rates and mitigating technologies Runway Status Lights (RWSLs) and Airport Surface Detection Equipment, Model X (ASDE-X), can help reduce runway incursions at severity levels A and B.
Structured-Light 3D Scanning as a Tool for Creating a Digital Collection of Modern and Fossil Cetacean Skeletons (Natural History Museum, University of Pisa)
Authors: Merella Marco , Farina Simone , Scaglia Patrizia , Caneve Gaia , Bernardini Giada , Pieri Alice , Collareta Alberto , Bianucci Giovanni
Published: October 2023

The Natural History Museum of the University of Pisa hosts one of the most important osteological collections of cetaceans all over Europe, as well as a conspicuous paleontological collection, including several holotypes of Archaeoceti (archaic whales), Mysticeti (baleen whales), and Odontoceti (toothed whales). In order to valorize these collections, we used 3D technologies to digitize the most relevant specimens, create an online archive, and make the resulting models accessible and shareable with the broadest audience possible through social media profiles and internet browsers. Tens of specimens were surface-scanned using a structured-light scanner, and the resulting 3D models were processed for post-production through the 3D software Blender whenever necessary. All the 3D scans were then gathered in the online repository Sketchfab, which was chosen for its user-friendly interface and common usage among museum institutions. The result is a web page that hosts 35 surface scans of extant and extinct cetacean specimens. This Sketchfab account was linked to the social media (Facebook and Instagram) profiles of the MSNUP to increase the visibility of the museum and promote the dissemination of its outstanding collections of modern and fossil cetaceans. The preliminary results of such an effort are encouraging in terms of views and online interactions. Hopefully, this effort of digitization and online archiving will soon extend to other vertebrate collections.
Assessing Medical Students’ Preferences for Rural Internships Using a Discrete Choice Experiment: A Case Study of Medical Students in a Public University in the Western Cape
Authors: Jose Maria , Obse Amarech , Zuidgeest Mark , Alaba Olufunke
Published: October 2023

As new graduates are crucial in providing healthcare services in rural areas, this study aimed to identify and describe the rural facility attributes that attract medical students to apply for rural internships. A literature review and focus groups informed a discrete choice experiment conducted amongst graduating medical students at one public university in South Africa. One main effect using a mixed logit model and another main effect plus interaction model was estimated. Females (130/66.33%) of urban origin (176/89.80%) with undergraduate exposure to rural facilities (110/56.12%) were the majority. The main effects only model showed advanced practical experience, hospital safety, correctly fitting personal protective equipment, and the availability of basic resources were the strongest predictors of rural internship uptake. Respondents were willing to forgo 66% of rural allowance (ZAR 2645.92, 95% CI: 1345.90; 3945.94) for a facility offering advanced practical experience. In contrast, increased rural allowance and housing provision were weak predictors of rural work uptake. Based on the interaction model, females and those not intending to specialise preferred hospital safety compared to advanced practical experience. To improve internship recruitment, rural facility managers should provide staff with supervision, safety, and protection from occupational exposure to contractible illnesses.
Identifying Urban Park Events through Computer Vision-Assisted Categorization of Publicly-Available Imagery
Authors: Tan Yizhou , Li Wenjing , Chen Da , Qiu Waishan
Published: October 2023

Understanding park events and their categorization offers pivotal insights into urban parks and their integral roles in cities. The objective of this study is to explore the efficacy of Convolutional Neural Networks (CNNs) in categorizing park events through images. Utilizing image and event category data from the New York City Parks Events Listing database, we trained a CNN model with the aim of enhancing the efficiency of park event categorization. While this study focuses on New York City, the approach and findings have the potential to offer valuable insights for urban planners examining park event distributions in different cities. Different CNN models were tuned to complete this multi-label classification task, and their performances were compared. Preliminary results underscore the efficacy of deep learning in automating the event classification process, revealing the multifaceted activities within urban green spaces. The CNN showcased proficiency in discerning various event nuances, emphasizing the diverse recreational and cultural offerings of urban parks. Such categorization has potential applications in urban planning, aiding decision-making processes related to resource distribution, event coordination, and infrastructure enhancements tailored to specific park activities.
Molecular and Cellular Insights: A Focus on Glycans and the HNK1 Epitope in Autism Spectrum Disorder
Authors: Hours Camille M. , Gil Sophie , Gressens Pierre
Published: October 2023

Autism Spectrum Disorder (ASD) is a synaptic disorder with a GABA/glutamate imbalance in the perineuronal nets and structural abnormalities such as increased dendritic spines and decreased long distance connections. Specific pregnancy disorders significantly increase the risk for an ASD phenotype such as preeclampsia, preterm birth, hypoxia phenomena, and spontaneous miscarriages. They are associated with defects in the glycosylation-immune placental processes implicated in neurogenesis. Some glycans epitopes expressed in the placenta, and specifically in the extra-villous trophoblast also have predominant functions in dendritic process and synapse function. Among these, the most important are CD57 or HNK1, CD22, CD24, CD33 and CD45. They modulate the innate immune cells at the maternal–fetal interface and they promote foeto-maternal tolerance. There are many glycan-based pathways of immunosuppression. N-glycosylation pathway dysregulation has been found to be associated with autoimmune-like phenotypes and maternal-autoantibody-related (MAR) autism have been found to be associated with central, systemic and peripheric autoimmune processes. Essential molecular pathways associated with the glycan-epitopes expression have been found to be specifically dysregulated in ASD, notably the Slit/Robo, Wnt, and mTOR/RAGE signaling pathways. These modifications have important effects on major transcriptional pathways with important genetic expression consequences. These modifications lead to defects in neuronal progenitors and in the nervous system’s implementation specifically, with further molecular defects in the GABA/glutamate system. Glycosylation placental processes are crucial effectors for proper maternofetal immunity and endocrine/paracrine pathways formation. Glycans/ galectins expression regulate immunity and neurulation processes with a direct link with gene expression. These need to be clearly elucidated in ASD pathophysiology.
Fear Conditioning by Proxy: The Role of High Affinity Nicotinic Acetylcholine Receptors
Authors: Chalkea Zinovia Stavroula , Papavranoussi-Daponte Danai , Polissidis Alexia , Kampisioulis Marinos , Pagaki-Skaliora Marina , Konsolaki Eleni , Skaliora Irini
Published: October 2023

Observational fear-learning studies in genetically modified animals enable the investigation of the mechanisms underlying the social transmission of fear-related information. Here, we used a three-day protocol to examine fear conditioning by proxy (FCbP) in wild-type mice (C57BL/6J) and mice lacking the β2-subunit of the nicotinic acetylcholine receptor (nAChR). Male animals of both genotypes were exposed to a previously fear-conditioned (FC) cage mate during the presentation of the conditioned stimulus (CS, tone). On the following day, observer (FCbP) mice were tested for fear reactions to the tone: none of the β2-KO mice froze to the stimulus, while 30% of the wild-type mice expressed significant freezing. An investigation of the possible factors that predicted the fear response revealed that only wild-type mice that exhibited enhanced and more flexible social interaction with the FC cage mate during tone presentations (Day 2) expressed fear toward the CS (Day-3). Our results indicate that (i) FCbP is possible in mice; (ii) the social transmission of fear depends on the interaction pattern between animals during the FCbP session and (iii) β2-KO mice display a more rigid interaction pattern compared to wild-type mice and are unable to acquire such information. These data suggest that β2-nAChRs influence observational fear learning indirectly through their effect on social behaviour.
Japanese Universities’ International Medical Partnerships: Reciprocity and Stratification
Authors: Kato Maki
Published: October 2023

Recently, study abroad and elective programs have been facilitated via university partnerships based on student-exchange agreements. This study examined international medical student exchange agreements, focusing on reciprocity and stratification in the Japanese context. An empirical analysis was conducted using 581 agreements involving student exchanges with medical institutions in foreign countries based on a survey conducted by the Japanese Ministry of Education. We found that the average reciprocity rate via mutual tuition waivers was 52.84%: equivalent in Asia, slightly lower in Europe (49.61%), and higher in North America (58.06%). Europe has a balanced inbound and outbound exchange, Asia has a higher inbound exchange, and North America has an excess of outbound exchange from Japan. Moreover, selective institutions, such as former imperial or medical universities, have more than twice the number of agreements per university compared to others. In conclusion, it can be observed that international medical university partnerships are stratified, and reciprocity is intertwined with tuition waivers and the number of exchange students in the partnering institutions. As the results highlight the distinct characteristics of international education partnerships in Japan’s medical field compared to partnerships in all academic fields, it is necessary to investigate and develop international partnerships separately by field.
Endoscopic Submucosal Dissection for Treatment of Early-Stage Cancer or Precancerous Lesion in the Upper Gastrointestinal Tract in Patients with Liver Cirrhosis
Authors: Tan Yuyong , Qing Yumin , Liu Deliang , Gong Jian
Published: October 2023

(1) Background: Endoscopic submucosal dissection (ESD) has been widely accepted as the standard method for treating early-stage cancer or precancerous lesions in the upper gastrointestinal tract; however, it may be difficult in patients with liver cirrhosis due to clinical challenges such as coagulation dysfunction, presence of gastroesophageal varices, etc. We aimed to demonstrate the safety and efficacy of ESD in these populations. (2) Methods: The clinical data of patients were retrospectively collected and analyzed. Inclusion criteria of the study were: a. patients with liver cirrhosis; b. patients who underwent ESD; c. patients who were diagnosed with early-stage cancer or precancerous lesions in the upper gastrointestinal tract. (3) Results: Eight patients were enrolled from April 2019 to April 2023, of whom three were male and five were female, with ages ranging from 43 to 70 years old. Seven lesions were located in the stomach and one other lesion was in the esophagus. ESD was performed successfully in all eight patients, and the resected lesion size ranged from 2 to 6 cm. Only one patient encountered postoperative complications, namely, chest pain and fever. No recurrence was noticed during a follow-up of 3 to 45 months. (4) Conclusions: ESD may serve as a safe and effective method for treating upper gastrointestinal early-stage cancer or precancerous lesions in patients with liver cirrhosis.
Ageing of Liquified Natural Gas during Marine Transportation and Assessment of the Boil-Off Thermodynamic Properties
Authors: Peruško Dalibor , Karabaić Damir , Bajsić Ivan , Kutin Jože
Published: October 2023

During LNG storage and transportation by ship, a fraction of the LNG in the cryogenic tanks evaporates due to heat ingress through the insulation, resulting in boil-off gas (BOG) production and a change in LNG composition, a phenomenon known as LNG ageing. Common practice is to assume that BOG composition and related density are identical to the initial LNG or pure methane, resulting in inaccuracy in BOG mass flow measurements. This is particularly important regarding LNG shipping economics and the utilization of BOG as a fuel for ship propulsion. This work investigates the influence of LNG ageing on the produced BOG thermodynamic properties relevant to the mentioned inaccuracies’ estimation and correction. An established, simplified, dynamic boil-off model is utilized for the simulation of LNG and BOG properties’ changes during the voyage. Four cases represented by limiting the minimum and maximum values of methane and nitrogen content are used to estimate the general influence of the compositional variability over the whole range of practically possible LNG source mixtures. Research results provide an insight into the relevant BOG properties’ variability and confirm that BOG flow measurements should be corrected with dynamic model simulations results due to significant differences between the total BOG mixture and forced BOG mixture corresponding to the LNG composition.
Emissions Assessment of Container Ships Sailing under Off-Design Conditions
Authors: Borén Clara , Grifoll Manel , Castells-Sanabra Marcella
Published: October 2023

Maritime transportation is the prevalent mode of transport for overseas freight, and it is frequently recognised as a relatively environmentally sustainable means of transport. However, shipping is still a substantial source of greenhouse gas emissions. We investigate the effect on fuel consumption and emissions when container ships are sailing below the design speed (i.e., slow steaming) as a strategy to minimise fuel consumption and costs. The estimation of ship fuel oil consumption is commonly based on the cubic speed‒power relation as a bottom-up approach. Nevertheless, the cubic relation could overestimate the impact of slow steaming on fuel consumption reduction and the emissions assessment. We compare real fuel consumption data and the consequent emissions with the results of assessing these parameters with the mentioned bottom-up approach. The analysis uses a set of container-ship slow steaming voyages, and the assessment is supported by speeds obtained from the Automatic Identification System (AIS). The exponential values obtained for the speed‒power relation range between 3.1 and 3.5, finding an overrating over 20% in all the cases analysed. Finally, we use a weather ship routing optimisation software to investigate additional emissions savings in the framework of ship-specific measures when weather ship routing and slow steaming are applied simultaneously.
Microbial Dysbiosis and Male Infertility: Understanding the Impact and Exploring Therapeutic Interventions
Authors: Kaltsas Aris , Zachariou Athanasios , Markou Eleftheria , Dimitriadis Fotios , Sofikitis Nikolaos , Pournaras Spyridon
Published: October 2023

The human microbiota in the genital tract is pivotal for maintaining fertility, but its disruption can lead to male infertility. This study examines the relationship between microbial dysbiosis and male infertility, underscoring the promise of precision medicine in this field. Through a comprehensive review, this research indicates microbial signatures associated with male infertility, such as altered bacterial diversity, the dominance of pathogenic species, and imbalances in the genital microbiome. Key mechanisms linking microbial dysbiosis to infertility include inflammation, oxidative stress, and sperm structural deterioration. Emerging strategies like targeted antimicrobial therapies, probiotics, prebiotics, and fecal microbiota transplantation have shown potential in adjusting the genital microbiota to enhance male fertility. Notably, the application of precision medicine, which customizes treatments based on individual microbial profiles and specific causes of infertility, emerges as a promising approach to enhance treatment outcomes. Ultimately, microbial dysbiosis is intricately linked to male infertility, and embracing personalized treatment strategies rooted in precision medicine principles could be the way forward in addressing infertility associated with microbial factors.
A Comparison of Hydrogen and Gasoline Piston Ring Simulations
Authors: Bewsher Stephen Richard , Offner Günter
Published: October 2023

This paper presents a transient mixed-lubrication hydrodynamic and gas flow simulation model for a piston ring pack for a four-stroke internal combustion engine. The analyses carried out compare two fuel types, hydrogen and gasoline, at a 2000 rpm low engine load (20%), as well as 3000 rpm low (20%) and high (100%) engine loads, to investigate the effects of the different fuels and loading conditions on the ring pack. In particular, the minimum oil film thickness at the top compression ring, the total ring friction of the ring pack, the friction power loss and the blow-by are studied. The simulation shows that, under the high load conditions at 3000 rpm, the hydrogen variant exhibits larger friction power losses, around a 200 W peak difference and larger blow-by throughout the expansion stroke of the engine cycle. A similar trend can be observed for the low loads, where larger friction power losses with peak differences of 30 W and 40 W for 2000 rpm and 3000 rpm, respectively, are observed. The blow-by results for the low load at 2000 rpm show a slight increase of approximately 22% more gas flow into the crankcase, while the 3000 rpm simulation shows a 50% increase in blow-by for the hydrogen variant at low load and a 40% increase at high load. The findings that are presented indicate that, although alternative fuel sources such as hydrogen are very attractive alternatives to fossil fuels such as gasoline, there can be unwanted side effects that could lead to the permanent damage of components through quicker wear or hydrogen embrittlement from the blow-by gas.
Diethyl (5-Benzyl-2-(4-(N′-hydroxycarbamimidoyl)phenyl)-5-methyl-4,5-dihydrofuran-3-yl)phosphonate
Authors: Avendaño Leon Oscar Leonardo , Curti Christophe , Santos Urbancg Moncorvo Fabiana Maia , Kabri Youssef , Redon Sébastien , Torres-Santos Eduardo Caio , Paoli-Lombardo Romain , Vanelle Patrice
Published: October 2023

As part of our ongoing research into the antileishmanial properties of amidoxime derivatives, we report a preliminary assessment of the antiparasitic properties of a novel compound, diethyl (5-benzyl-2-(4-(N′-hydroxycarbamimidoyl)phenyl)-5-methyl-4,5-dihydrofuran-3-yl)phosphonate. This compound was evaluated in vitro for the first time against the promastigote form of Leishmania amazonensis. Compounds containing both amidoxime and phosphonyl functional groups in dihydrofuran scaffolds are relatively rare, despite the extensive study of this heterocycle in various biological applications. Therefore, this work makes a valuable contribution to the fight against Leishmania spp. as a neglected disease. The cyclized 4,5-dihydrofuran intermediate scaffold was obtained via a three-step synthetic route that had previously been developed for accessing other derivatives, including the sulfone moiety. This synthesis was performed using a manganese-based free radical oxidative method under microwave irradiation. The intermediary 4,5-dihydrofuran, which included a nitrile group, tolerated the subsequent reaction with hydroxylamine hydrochloride, resulting in the formation of the target product. The target compound showed moderate activity in vitro against the promastigote form of L. amazonensis (IC50 = 91.1 µM).
Anisotropic Properties of Epitaxial Ferroelectric Lead-Free 0.5[Ba(Ti0.8Zr0.2)O3]-0.5(Ba0.7Ca0.3)TiO3 Films
Authors: Cucciniello Nicholas , Mazza Alessandro R. , Roy Pinku , Kunwar Sundar , Zhang Di , Feng Henry Y. , Arsky Katrina , Chen Aiping , Jia Quanxi
Published: October 2023

As the energy demand is expected to double over the next 30 years, there has been a major initiative towards advancing the technology of both energy harvesting and storage for renewable energy. In this work, we explore a subset class of dielectrics for energy storage since ferroelectrics offer a unique combination of characteristics needed for energy storage devices. We investigate ferroelectric lead-free 0.5[Ba(Ti0.8Zr0.2)O3]-0.5(Ba0.7Ca0.3)TiO3 epitaxial thin films with different crystallographic orientations grown by pulsed laser deposition. We focus our attention on the influence of the crystallographic orientation on the microstructure, ferroelectric, and dielectric properties. Our results indicate an enhancement of the polarization and strong anisotropy in the dielectric response for the (001)-oriented film. The enhanced ferroelectric, energy storage, and dielectric properties of the (001)-oriented film is explained by the coexistence of orthorhombic-tetragonal phase, where the disordered local structure is in its free energy minimum.
The Memristive Properties and Spike Timing-Dependent Plasticity in Electrodeposited Copper Tungstates and Molybdates
Authors: Przyczyna Dawid , Mech Krzysztof , Kowalewska Ewelina , Marzec Mateusz , Mazur Tomasz , Zawal Piotr , Szaciłowski Konrad
Published: October 2023

Memristors possess non-volatile memory, adjusting their electrical resistance to the current that flows through them and allowing switching between high and low conducting states. This technology could find applications in fields such as IT, consumer electronics, computing, sensors, and medicine. In this paper, we report successful electrodeposition of thin-film materials consisting of copper tungstate and copper molybdate (CuWO4 and Cu3Mo2O9), which showed notable memristive properties. Material characterisation was performed with techniques such as XRD, XPS, and SEM. The electrodeposited materials exhibited the ability to switch between low and high resistive states during varied cyclic scans and short-term impulses. The retention time of these switched states was also explored. Using these materials, the effects seen in biological systems, specifically spike timing-dependent plasticity, were simulated, being based on analogue operation of the memristors to achieve multiple conductivity states. Bio-inspired simulations performed directly on the material could possibly offer energy and time savings for classical computations. Memristors could be crucial for the advancement of high-efficiency, low-energy neuromorphic electronic devices and technologies in the future.
NiTi2, a New Liquid Glass
Authors: Tournier Robert F. , Ojovan Michael I.
Published: October 2023

Many endothermic liquid–liquid transitions, occurring at a temperature Tn+ above the melting temperature Tm, are related to previous exothermic transitions, occurring at a temperature Tx after glass formation below Tg, with or without attached crystallization and predicted by the nonclassical homogenous nucleation equation. A new thermodynamic phase composed of broken bonds (configurons), driven by percolation thresholds, varying from ~0.145 to Δε, is formed at Tx, with a constant enthalpy up to Tn+. The liquid fraction Δε is a liquid glass up to Tn+. The solid phase contains glass and crystals. Molecular dynamics simulations are used to induce, in NiTi2, a reversible first-order transition by varying the temperature between 300 and 1000 K under a pressure of 1000 GPa. Cooling to 300 K, without applied pressure, shows the liquid glass presence with Δε = 0.22335 as memory effect and Tn+ = 2120 K for Tm = 1257 K.
Joint Statistics of Partial Sums of Ordered i.n.d. Gamma Random Variables
Authors: Nam Sung Sik , Yoon Changseok , Choi Seyeong
Published: October 2023

From the perspective of wireless communication, as communication systems become more complex, order statistics have gained increasing importance, particularly in evaluating the performance of advanced technologies in fading channels. However, existing analytical methods are often too complex for practical use. In this research paper, we introduce innovative statistical findings concerning the sum of ordered gamma-distributed random variables. We examine various channel scenarios where these variables are independent but not-identically distributed. To demonstrate the practical applicability of our results, we provide a comprehensive closed-form expression for the statistics of the signal-to-interference-plus-noise ratio in a multiuser scheduling system. We also present numerical examples to illustrate the effectiveness of our approach. To ensure the accuracy of our analysis, we validate our analytical results through Monte Carlo simulations.
Ultimate Dynamics of the Two-Phenotype Cancer Model: Attracting Sets and Global Cancer Eradication Conditions
Authors: Kanatnikov Anatolij N. , Starkov Konstantin E.
Published: October 2023

In this paper we consider the ultimate dynamics of one 4D cancer model which was created for studying the immune response to the two-phenotype tumors. Our approach is based on the localization method of compact invariant sets. The existence of a positively invariant polytope is shown and its size is calculated depending on the parameters of this cancer model. Various convergence conditions to the tumor free equilibrium point were proposed. This property has the biological meaning of global asymptotic tumor eradication (GATE). Further, the case in which local asymptotic tumor eradication (LATE) conditions entail GATE conditions was found. Our theoretical studies of ultimate dynamics are complemented by numerical simulation results.
Large Intron Inversions in Romanian Patients with Hemophilia A—First Report
Authors: Brinza Melen , Grigore Andra , Dragomir Mihaela , Jardan Dumitru , Jardan Cerasela , Balanescu Paul , Tarniceriu Claudia Cristina , Badulescu Oana Viola , Blag Cristina , Tomuleasa Ciprian , Traila Adina , Serban Margit , Coriu Daniel
Published: October 2023

Background and Objectives: Despite the vast heterogeneity in the genetic defects causing hemophilia A (HA), large intron inversions represent a major cause of disease, accounting for almost half of the cases of severe HA worldwide. We investigated the intron 22 and intron 1 inversion status in a cohort of Romanian unrelated patients with severe HA. Moreover, we evaluated the role of these inversions as relative risk factors in inhibitor occurrence. Materials and Methods: Inverse shifting—a polymerase chain reaction method was used to detect the presence of intron 22 and intron 1 inversions in 156 Romanian patients with HA. Results: Intron inversion 22 was found in 41.7% of the patients, while intron 1 inversion was detected in 3.2% of the patients. Overall, large intron inversions represented the molecular defect in 44.9% of the studied patients. Our findings are in accord with previously published reports from Eastern Europe countries and with other international studies. The risk of inhibitor development was higher in patients with inversion 1 compared to the patients with HA without any inversion detected. Conclusions: The current study demonstrates the major causative role of large intron inversions in severe HA in Romanian patients. Moreover, our study confirms the contribution of intron 1 inversion in inhibitor development.
Trends of Coagulation Parameters in Human Immunodeficiency Virus Patients
Authors: Bashir Bashir Abdrhman , Mohamed Mohamed Hassan , Hussain Mohamed A. , Osman Wadah , Mothana Ramzi A. , Hasson Sidgi
Published: October 2023

Background and Objectives: HIV disease is recognized to cause inconsistencies in coagulation via various pathways during infection. Some studies have indicated that HIV-infected patients are prone to developing thrombocytopenia, thrombosis, or autoantibodies that may cause difficulties in diagnosis. This study is intended to measure the trend of coagulation parameters in Sudanese patients with HIV. Materials and Methods: A cross-sectional study was carried out in patients with HIV admitted to the Sudan National AIDS Program (SNAP) from January 2018 to December 2019. Prothrombin time (PT), partial thromboplastin time (PTT), thrombin time (TT), D-dimer (DD), hemoglobin (HB), total lymphocyte count (TLC), platelet count (PLT), and a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13 (ADAMTS13), were evaluated among HIV Sudanese patients. Results: Out of the 44 HIV patients included, 6 (13.6%) were found to have thrombotic thrombocytopenic purpura-like events and 12 (27.2%) had antiphospholipid antibodies, of whom 8 (66.6%) showed anticardiolipin antibody (1gG (75%) and IgM (25%)) and 4 showed lupus anticoagulants. The HB, TLC, and PLT values were found to be significantly lower in HIV patients than in control (p = 0.000, 0.000, and 0.050, respectively). The PT and ADAMTS13 values showed no significant difference between HIV patients and control (p = 0.613 and 0.266, respectively). The PTT, TT, and DD values were found to be augmented in HIV patients versus the control (p = 0.000). Conclusions: Thrombotic thrombocytopenic purpura-like events among HIV Sudanese patients were explored. In addition, antiphospholipid antibodies were strikingly seen in these patients. Additional research is anticipated to confirm these diagnoses.
The Effects of a Short-Term Combined Exercise Program on Liver Steatosis Indices and the Lipidemic and Glycemic Profile in NAFLD Individuals: A Pilot Study
Authors: Voudouris Dimitrios , Horianopoulou Maria , Apostolopoulou Zoi , Chryssanthopoulos Costas , Bardopoulou Mari , Maridaki Maria , Vassilakopoulos Theodoros , Koutsilieris Michael , Philippou Anastassios
Published: October 2023

Non-alcoholic fatty liver disease (NAFLD) is a very common liver disease associated with obesity, unhealthy diet, and lack of physical exercise. Short-term aerobic or resistance exercise has been shown to result in reduced liver fat in patients with NAFLD; however, the impact of the combination of these types of exercise has received less attention. This study investigated the effect of a short-term (7 days) concurrent exercise training program performed daily on liver steatosis indices, as well as the glycemic and lipidemic profile of overweight/obese sedentary volunteers. Twenty adult patients (age: 47.3 ± 12.3 yrs, body mass index: 32.4 ± 3.4 kg/m2) with NAFLD, detected by ultrasound and hematological indices, participated in the study. Pre- and post-exercise intervention assessment included body weight (BW), waist circumference (WC), hip/waist ratio (H/W), Homeostasis Model Assessment Insulin Resistance (HOMA-IR), blood lipids, and steatosis indices. Fatty Liver Index, Lipid Accumulation Index, WC, H/W, triglycerides, and total cholesterol were improved (p < 0.05) post-exercise, while no differences (p > 0.05) were observed in BW, HOMA-IR, HDL, LDL, Hepatic Steatosis Index, and Framingham Steatosis Index compared to pre-exercise values. It is concluded that a 7-day combined exercise program can have beneficial effects on hepatic steatosis and central adiposity indices, independently of weight loss, in patients with NAFLD.
Cytokinin Translocation to, and Biosynthesis and Metabolism within, Cereal and Legume Seeds: Looking Back to Inform the Future
Authors: Jameson Paula E.
Published: October 2023

Early in the history of cytokinins, it was clear that Zea mays seeds contained not just trans-zeatin, but its nucleosides and nucleotides. Subsequently, both pods and seeds of legumes and cereal grains have been shown to contain a complex of cytokinin forms. Relative to the very high quantities of cytokinin detected in developing seeds, only a limited amount appears to have been translocated from the parent plant. Translocation experiments, and the detection of high levels of endogenous cytokinin in the maternal seed coat tissues of legumes, indicates that cytokinin does not readily cross the maternal/filial boundary, indicating that the filial tissues are autonomous for cytokinin biosynthesis. Within the seed, trans-zeatin plays a key role in sink establishment and it may also contribute to sink strength. The roles, if any, of the other biologically active forms of cytokinin (cis-zeatin, dihydrozeatin and isopentenyladenine) remain to be elucidated. The recent identification of genes coding for the enzyme that leads to the biosynthesis of trans-zeatin in rice (OsCYP735A3 and 4), and the identification of a gene coding for an enzyme (CPN1) that converts trans-zeatin riboside to trans-zeatin in the apoplast, further cements the key role played by trans-zeatin in plants.
A Class 4-like Chromosomal Integron Found in Aeromonas sp. Genomospecies paramedia Isolated from Human Feces
Authors: Baltazar-Cruz Jesús , Rojas-Rios Rogelio , Larios-Serrato Violeta , Mendoza-Sanchez Itza , Curiel-Quesada Everardo , Pérez-Valdespino Abigail
Published: October 2023

Integrons are genetic elements that store, express and exchange gene cassettes. These elements are characterized by containing a gene that codes for an integrase (intI), a cassette integration site (attI) and a variable region holding the cassettes. Using bioinformatics and molecular biology methods, a functional integron found in Aeromonas sp. 3925, a strain isolated from diarrheal stools, is described. To confirm the integron class, a phylogenetic analysis with amino acid sequences was conducted. The integrase was associated to class 4 integrases; however, it is clearly different from them. Thus, we classified the associated element as a class 4-like integron. We found that the integrase activity is not under the control of the SOS or catabolic repression, since the expression was not increased in the presence of mitomycin or arabinose. The class-4-like integron is located on the chromosome and contains two well-defined gene cassettes: aadA1 that confers resistance to streptomycin and lpt coding for a lipoprotein. It also includes eight Open Reading frames (ORFs) with unknown functions. The strain was characterized through a Multilocus Phylogenetic Analyses (MLPA) of the gyrB, gyrA, rpoD, recA, dnaJ and dnaX genes. The phylogenetic results grouped it into a different clade from the species already reported, making it impossible to assign a species. We resorted to undertaking complete genome sequencing and a phylogenomic analysis. Aeromonas sp. 3925 is related to A. media and A. rivipollensis clusters, but it is clearly different from these species. In silico DNA-DNA hybridization (isDDH) and Average Nucleotide Identity (ANI) analyses suggested that this isolate belongs to the genomospecies paramedia. This paper describes the first class 4-like integron in Aeromonas and contributes to the establishment of genomospecies paramedia.
Phenotypic and Genomic Characterization of Streptomyces pakalii sp. nov., a Novel Species with Anti-Biofilm and Anti-Quorum Sensing Activity in ESKAPE Bacteria
Authors: Chávez-Hernández Michelle , Ortiz-Álvarez Jossue , Morales-Jiménez Jesús , Villa-Tanaca Lourdes , Hernández-Rodríguez César
Published: October 2023

The increasing number of infections caused by antimicrobial multi-resistant microorganisms has led to the search for new microorganisms capable of producing novel antibiotics. This work proposes Streptomyces pakalii sp. nov. as a new member of the Streptomycetaceae family. The strain ENCB-J15 was isolated from the jungle soil in Palenque National Park, Chiapas, Mexico. The strain formed pale brown, dry, tough, and buried colonies in the agar with no diffusible pigment in GAE (glucose–asparagine–yeast extract) medium. Scanning electron micrographs showed typical mycelium with long chains of smooth and oval-shaped spores (3–10 m). The strain grew in all of the International Streptomyces Project (ISP)’s media at 28–37 °C with a pH of 6–9 and 0–10% NaCl. S. pakalii ENCB-J15 assimilated diverse carbon as well as organic and inorganic nitrogen sources. The strain also exhibited significant inhibitory activity against the prodigiosin synthesis of Serratia marcescens and the inhibition of the formation and destruction of biofilms of ESKAPE strains of Acinetobacter baumannii and Klebsiella pneumoniae. The draft genome sequencing of ENCB-J15 revealed a 7.6 Mb genome with a high G + C content (71.6%), 6833 total genes, and 6746 genes encoding putative proteins. A total of 26 accessory clusters of proteins associated with carbon sources and amino acid catabolism, DNA modification, and the antibiotic biosynthetic process were annotated. The 16S rRNA gene phylogeny, core-proteome phylogenomic tree, and virtual genome fingerprints support that S. pakalii ENCB-J15 is a new species related to Streptomyces badius and Streptomyces globisporus. Similarly, its average nucleotide identity (ANI) (96.4%), average amino acid identity (AAI) (96.06%), and virtual DNA–DNA hybridization (67.3%) provide evidence to recognize it as a new species. Comparative genomics revealed that S. pakalli and its closest related species maintain a well-conserved genomic synteny. This work proposes Streptomyces pakalii sp. nov. as a novel species that expresses anti-biofilm and anti-quorum sensing activities.