bluuebunny/crossref_metadata_2025 huggingface dataset:
Secondary Bile Acids and Short Chain Fatty Acids in the Colon: A Focus on Colonic Microbiome, Cell Proliferation, Inflammation, and Cancer
Authors: Zeng Huawei , Umar Shahid , Rust Bret , Lazarova Darina , Bordonaro Michael
Published: March 2019

Secondary bile acids (BAs) and short chain fatty acids (SCFAs), two major types of bacterial metabolites in the colon, cause opposing effects on colonic inflammation at chronically high physiological levels. Primary BAs play critical roles in cholesterol metabolism, lipid digestion, and host–microbe interaction. Although BAs are reabsorbed via enterohepatic circulation, primary BAs serve as substrates for bacterial biotransformation to secondary BAs in the colon. High-fat diets increase secondary BAs, such as deoxycholic acid (DCA) and lithocholic acid (LCA), which are risk factors for colonic inflammation and cancer. In contrast, increased dietary fiber intake is associated with anti-inflammatory and anticancer effects. These effects may be due to the increased production of the SCFAs acetate, propionate, and butyrate during dietary fiber fermentation in the colon. Elucidation of the molecular events by which secondary BAs and SCFAs regulate colonic cell proliferation and inflammation will lead to a better understanding of the anticancer potential of dietary fiber in the context of high-fat diet-related colon cancer. This article reviews the current knowledge concerning the effects of secondary BAs and SCFAs on the proliferation of colon epithelial cells, inflammation, cancer, and the associated microbiome.
Comparative Gene-Expression Analysis of Alzheimer’s Disease Progression with Aging in Transgenic Mouse Model
Authors: Abid Noman Bin , Naseer Muhammad Imran , Kim Myeong Ok
Published: March 2019

Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder characterized by progressive memory dysfunction and a decline in cognition. One of the biggest challenges to study the pathological process at a molecular level is that there is no simple, cost-effective, and comprehensive gene-expression analysis tool. The present study provides the most detailed (Reverse transcription polymerase chain reaction) RT-PCR-based gene-expression assay, encompassing important genes, based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) disease pathway. This study analyzed age-dependent disease progression by focusing on pathological events such as the processing of the amyloid precursor protein, tau pathology, mitochondrial dysfunction, endoplasmic reticulum stress, disrupted calcium signaling, inflammation, and apoptosis. Messenger RNA was extracted from the cortex and hippocampal region of APP/PS1 transgenic mice. Samples were divided into three age groups, six-, nine-, and 12-month-old transgenic mice, and they were compared with normal C57BL/6J mice of respective age groups. Findings of this study provide the opportunity to design a simple, effective, and accurate clinical analysis tool that can not only provide deeper insight into the disease, but also act as a clinical diagnostic tool for its better diagnosis.
Novichoks: The Dangerous Fourth Generation of Chemical Weapons
Authors: Franca Tanos C. C. , Kitagawa Daniel A. S. , Cavalcante Samir F. de A. , da Silva Jorge A. V. , Nepovimova Eugenie , Kuca Kamil
Published: March 2019

“Novichoks” is the name given to the controversial chemical weapons supposedly developed in the former Soviet Union between the 1970s and the 1990s. Designed to be undetectable and untreatable, these chemicals became the most toxic of the nerve agents, being very attractive for both terrorist and chemical warfare purposes. However, very little information is available in the literature, and the Russian government did not acknowledge their development. The intent of this review is to provide the IJMS readers with a general overview on what is known about novichoks today. We briefly tell the story of the secret development of these agents, and discuss their synthesis, toxicity, physical-chemical properties, and possible ways of treatment and neutralization. In addition, we also wish to call the attention of the scientific community to the great risks still represented by nerve agents worldwide, and the need to keep constant investments in the development of antidotes and ways to protect against such deadly compounds.
Dietary Eriodictyol Alleviates Adiposity, Hepatic Steatosis, Insulin Resistance, and Inflammation in Diet-Induced Obese Mice
Authors: Kwon Eun-Young , Choi Myung-Sook
Published: March 2019

The present study aimed to investigate the molecular mechanisms underlying the anti-obesity effect of flavonoid eriodictyol (ED) supplementation in mice fed with a high-fat diet (HFD). C57BL/6N mice were fed with normal diet (ND), HFD (40 kcal% fat), or HFD + 0.005% (w/w) ED for 16 weeks. In HFD-induced obese mice, dietary ED supplementation significantly alleviated dyslipidemia and adiposity by downregulating the expression of lipogenesis-related genes in white adipose tissue (WAT), while enhancing fecal lipid excretion. ED additionally improved hepatic steatosis and decreased the production of pro-inflammatory cytokines by downregulating the expression of hepatic enzymes and the genes involved in lipogenesis and upregulating the expression of hepatic fatty acid oxidation-related enzymes and genes. In addition, ED improved insulin resistance (IR) by suppressing hepatic gluconeogenesis, enhancing glucose utilization, and modulating the production and release of two incretin hormones, namely gastric inhibitory polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). Taken together, the current findings indicated that ED can protect against diet-induced obesity and related metabolic disturbances, including dyslipidemia, inflammation, fatty liver disease, and IR in diet-induced obese mice.
Machine Learning Models for Error Detection in Metagenomics and Polyploid Sequencing Data
Authors: Krachunov Milko , Nisheva Maria , Vassilev Dimitar
Published: March 2019

Metagenomics studies, as well as genomics studies of polyploid species such as wheat, deal with the analysis of high variation data. Such data contain sequences from similar, but distinct genetic chains. This fact presents an obstacle to analysis and research. In particular, the detection of instrumentation errors during the digitalization of the sequences may be hindered, as they can be indistinguishable from the real biological variation inside the digital data. This can prevent the determination of the correct sequences, while at the same time make variant studies significantly more difficult. This paper details a collection of ML-based models used to distinguish a real variant from an erroneous one. The focus is on using this model directly, but experiments are also done in combination with other predictors that isolate a pool of error candidates.
Tuning the Linear and Nonlinear Optical Properties of Pyrene-Pyridine Chromophores by Protonation and Complexation to d10 Metal Centers §
Authors: Lucenti Elena , Forni Alessandra , Marinotto Daniele , Previtali Andrea , Righetto Stefania , Cariati Elena
Published: March 2019

The linear and second-order nonlinear optical (NLO) properties of two pyrene-pyridine chromophores, namely, 4-(pyren-1-yl)pyridine (L1) and 4-(2-(pyren-1-yl)ethyl)pyridine (L2), were investigated and modulated by performing protonation/deprotonation cycles or by complexation to d10 metal centers such as Zn(II) and Cu(I) to form the monomeric [Zn(CH3CO2)2(L1)2] complex and the [CuI(L2)]n coordination polymer, respectively. The structures of L1, L2, [Zn(CH3CO2)2(L1)2] and [CuI(L2)]n were determined by means of single-crystal X-ray diffraction studies. The NLO response, measured by the electric-field-induced second harmonic generation (EFISH) technique, was positive for both chromophores and showed an inversion of the sign after exposure to HCl vapors. This process was completely reversible and the original values were restored by simple exposure to NH3 vapors. Coordination of L1 to Zn(II) also resulted in a negative NLO response, although smaller in magnitude compared to the protonated form, due to the weak Lewis acidity of the “Zn(CH3CO2)2” fragment. The results were also interpreted on the basis of DFT/TDDFT calculations.
Genome-Wide Copy Number Variation Association Study of Atrial Fibrillation Related Thromboembolic Stroke
Authors: Hsieh Chia-Shan , Huang Pang-Shuo , Chang Sheng-Nan , Wu Cho-Kai , Hwang Juey-Jen , Chuang Eric Y. , Tsai Chia-Ti
Published: March 2019

Atrial fibrillation (AF) is a common cardiac arrhythmia and is one of the major causes of ischemic stroke. In addition to the clinical factors such as CHADS2 or CHADS2-VASC score, the impact of genetic factors on the risk of thromboembolic stroke in patients with AF has been largely unknown. Single-nucleotide polymorphisms in several genomic regions have been found to be associated with AF. However, these loci do not contribute to all the genetic risks of AF or AF related thromboembolic risks, suggesting that there are other genetic factors or variants not yet discovered. In the human genome, copy number variations (CNVs) could also contribute to disease susceptibility. In the present study, we sought to identify CNVs determining the AF-related thromboembolic risk. Using a genome-wide approach in 109 patients with AF and thromboembolic stroke and 14,666 controls from the Taiwanese general population (Taiwan Biobank), we first identified deletions in chromosomal regions 1p36.32-1p36.33, 5p15.33, 8q24.3 and 19p13.3 and amplifications in 14q11.2 that were significantly associated with AF-related stroke in the Taiwanese population. In these regions, 148 genes were involved, including several microRNAs and long non-recoding RNAs. Using a pathway analysis, we found deletions in GNB1, PRKCZ, and GNG7 genes related to the alpha-adrenergic receptor signaling pathway that play a major role in determining the risk of an AF-related stroke. In conclusion, CNVs may be genetic predictors of a risk of a thromboembolic stroke for patients with AF, possibly pointing to an impaired alpha-adrenergic signaling pathway in the mechanism of AF-related thromboembolism.
Serum Zinc Concentration and Sarcopenia: A Close Linkage in Chronic Liver Diseases
Authors: Nishikawa Hiroki , Enomoto Hirayuki , Yoh Kazunori , Iwata Yoshinori , Sakai Yoshiyuki , Kishino Kyohei , Ikeda Naoto , Takashima Tomoyuki , Aizawa Nobuhiro , Takata Ryo , Hasegawa Kunihiro , Ishii Noriko , Yuri Yukihisa , Nishimura Takashi , Iijima Hiroko , Nishiguchi Shuhei
Published: March 2019

We sought to investigate the influence of serum zinc (Zn) concentration on sarcopenia in chronic liver diseases (CLDs, n = 372, median age = 65 years, 147 liver cirrhosis (LC) cases (39.5%)). Sarcopenia was defined by low grip strength and low skeletal muscle mass. Study subjects were divided into the following three groups (High-, Intermediate-, and Low-Zn groups) based on the baseline serum Zn level. The impacts of serum Zn concentration on sarcopenia were examined. The median (interquartile range) serum Zn concentration for all cases was 72.85 (63.7, 81.45) μg/dL. The proportions of sarcopenia in the High-Zn, Intermediate-Zn, and Low-Zn groups were 10.75% (10/93), 11.23% (21/187), and 27.17% (25/92), respectively (P = 0.9046 (High vs. Intermediate), P = 0.0007 (Intermediate vs. Low), P = 0.0044 (High vs. Low), overall P value = 0.0009). The median serum Zn concentrations in patients with sarcopenia, pre-sarcopenia, and control were 66.35, 73.1 and 73.8 μg/dL, respectively (P = 0.0234 (sarcopenia vs. pre-sarcopenia), P = 0.2116 (pre-sarcopenia vs. control), P = 0.0002 (sarcopenia vs. control), overall P value = 0.0016). In the multivariate analyses of factors linked to the presence of sarcopenia, Low-Zn was an independent predictor for all cases (P = 0.0236) and LC cases (P = 0.0082). In conclusion, Zn deficiency can be an independent predictor for sarcopenia in patients with CLDs.
Effect of Androgen Deprivation Therapy on Other-Cause of Mortality in Elderly Patients with Clinically Localized Prostate Cancer Treated with Modern Radiotherapy: Is There a Negative Impact?
Authors: Yamazaki Hideya , Masui Koji , Suzuki Gen , Nakamura Satoaki , Aibe Norihiro , Shimizu Daisuke , Yamada Kei , Okihara Koji , Shiraishi Takumi , Kotsuma Tadayuki , Yoshida Ken , Tanaka Eiichi , Otani Keisuke , Yoshioka Yasuo , Ogawa Kazuhiko , Nishikawa Tatsuyuki , Okabe Haruumi
Published: March 2019

The influence of androgen deprivation therapy (ADT) on other-cause of mortality (OCM) was investigated in patients with localized prostate cancer treated with modern high-dose radiotherapy. A retrospective review was conducted on 1125 patients with localized prostate cancer treated with high-dose radiotherapy, including image-guided, intensity-modulated radiotherapy or brachytherapy with a median follow-up of 80.7 months. Overall survival rate was no different between ADT (+) and ADT (−) group in high-, intermediate-, and low-risk groups. OCM was found in 71 patients, consisting of 4% (10/258) in the ADT (−) group and 7% (61/858) in the ADT (+) group (p = 0.0422). The 10-year OCM-free survival rate (OCMFS), if divided by the duration of ADT (ADT naïve (ADT (−)), ADT <2-year, and ADT ≥2-year groups), showed statistical significance, and was 90.7%, 88.2%, and 78.6% (p = 0.0039) for the ADT (−), ADT <2-year, and ADT ≥2-year groups, respectively. In patients aged ≥75 years, 10-year OCMFS for ADT (−), ADT <2-, and ADT ≥2-year groups was 93.5% (at 115.6 months), 85.6%, and 60.7% (p = 0.0189), respectively, whereas it was 90.7%, 89.9%, and 89.0% (p = 0.4716), respectively, in their younger counterparts. In localized prostate cancer patients, treatment with longer ADT for ≥2 years potentially increases the risk of OCM, especially in patients aged ≥75 years.
Analysis of Image Feature Characteristics for Automated Scoring of HER2 in Histology Slides
Authors: Mukundan Ramakrishnan
Published: March 2019

The evaluation of breast cancer grades in immunohistochemistry (IHC) slides takes into account various types of visual markers and morphological features of stained membrane regions. Digital pathology algorithms using whole slide images (WSIs) of histology slides have recently been finding several applications in such computer-assisted evaluations. Features that are directly related to biomarkers used by pathologists are generally preferred over the pixel values of entire images, even though the latter has more information content. This paper explores in detail various types of feature measurements that are suitable for the automated scoring of human epidermal growth factor receptor 2 (HER2) in histology slides. These are intensity features known as characteristic curves, texture features in the form of uniform local binary patterns (ULBPs), morphological features specifying connectivity of regions, and first-order statistical features of the overall intensity distribution. This paper considers important properties of the above features and outlines methods for reducing information redundancy, maximizing inter-class separability, and improving classification accuracy in the combined feature set. This paper also presents a detailed experimental analysis performed using the aforementioned features on a WSI dataset of IHC stained slides.
Experimental Evaluation of SAFEPOWER Architecture for Safe and Power-Efficient Mixed-Criticality Systems
Authors: Fakih Maher , Grüttner Kim , Schreiner Sören , Seyyedi Razi , Azkarate-Askasua Mikel , Onaindia Peio , Poggi Tomaso , Romero Nera González , Quesada Gonzalez Elena , Sundström Timmy , Peiró Frasquet Salvador , Balbastre Patricia , Mohammadat Tage , Öberg Johnny , Bebawy Yosab , Obermaisser Roman , Maleki Adele , Lenz Alina , Graham Duncan
Published: March 2019

With the ever-increasing industrial demand for bigger, faster and more efficient systems, a growing number of cores is integrated on a single chip. Additionally, their performance is further maximized by simultaneously executing as many processes as possible. Even in safety-critical domains like railway and avionics, multicore processors are introduced, but under strict certification regulations. As the number of cores is continuously expanding, the importance of cost-effectiveness grows. One way to increase the cost-efficiency of such a System on Chip (SoC) is to enhance the way the SoC handles its power consumption. By increasing the power efficiency, the reliability of the SoC is raised because the lifetime of the battery lengthens. Secondly, by having less energy consumed, the emitted heat is reduced in the SoC, which translates into fewer cooling devices. Though energy efficiency has been thoroughly researched, there is no application of those power-saving methods in safety-critical domains yet. The EU project SAFEPOWER (Safe and secure mixed-criticality systems with low power requirements) targets this research gap and aims to introduce certifiable methods to improve the power efficiency of mixed-criticality systems. This article provides an overview of the SAFEPOWER reference architecture for low-power mixed-criticality systems, which is the most important outcome of the project. Furthermore, the application of this reference architecture in novel railway interlocking and flight controller avionic systems was demonstrated, showing the capability to achieve power savings up to 37%, while still guaranteeing time-triggered task execution and time-triggered NoC-based communication.
The Role of Entrepreneurial Strategy, Network Ties, Human and Financial Capital in New Venture Performance
Authors: Khan Najib Ullah , Li Shuangjie , Safdar Muhammad Nabeel , Khan Zia Ullah
Published: March 2019

In the current era of globalization and competitive edge, the survival of newly established ventures has become a big challenge. Numerous studies have been carried out to discover factors that are essential for newly initiated ventures but the results are still fragmented. This study focuses on measuring the effect of entrepreneurial strategy, network ties, human capital and financial capital on new venture performance. A structured questionnaire was used to collect data from 196 registered firms located in the emerging market Pakistan. The results indicate that entrepreneurial strategy, network ties and financial capital have a significant positive effect, while human capital showed an insignificant effect on new venture performance. This research recommends owners and managers of new firms build effective entrepreneurial strategies, expand their networks with external bodies (other firms, government and financial institutions) to acquire useful resources that in turn can spur their performance. Further implications are discussed. Policy makers and responsible authorities are advised to encourage and support new ventures which in turn can contribute to GDP and economic development. Practical implications and suggestions are also discussed.
Effect of Ru, Rh, Mo, and Pd Adsorption on the Electronic and Optical Properties of Anatase TiO2(101): A DFT Investigation
Authors: Gao Peng , Yang Libin , Xiao Songtao , Wang Lingyu , Guo Wei , Lu Jinghao
Published: March 2019

Adsorbed metal atoms and metal doping onto TiO2 can effectively enhance the optical and photocatalytic activity of photocatalytic efficiency of titanium dioxide (TiO2), favoring the extension of its optical absorption spectrum and the efficiency of hydrogen generation. To investigate the possible mechanism causing potential improvement of photocatalytic activity, the electronic and optical properties of the anatase TiO2(101) plane with different adsorbed metal atom have been theoretically calculated through density functional theory (DFT) method. Adsorption of Pd and Ru atoms increases the delocalization of the density of states, with an impurity state near the Fermi level. Moreover, the investigated adsorbed metal atoms (Mo, Pd, Ru, Rh) narrow the band gap of anatase TiO2, thus enhancing the probability of photoactivation by visible light. The orbital hybridization of the d orbit from the adsorbed metal atom and the p orbit from the O of the defect site increases the Schottky barrier of the electronic structure.
Correction: Ungson, Y. et al. Filling of Irregular Channels with Round Cross-Section: Modeling Aspects to Study the Properties of Porous Materials. Materials 2018, 11, 1901
Authors: Ungson Yamel , Burtseva Larysa , Garcia-Curiel Edwin R. , Valdez Salas Benjamin , Flores-Rios Brenda L. , Werner Frank , Petranovskii Vitalii
Published: March 2019

The authors have found two errors in the paper published in Materials [...]
Densification Mechanism of Soft Magnetic Composites Using Ultrasonic Compaction for Motors in EV Platforms
Authors: Hwang Myeong-Hwan , Lee Hae-Sol , Han Jong-Ho , Kim Dong-Hyun , Cha Hyun-Rok
Published: March 2019

In this paper, the densification mechanism of ultrasonic compaction was analyzed using a force balance model. Ultrasonic compaction is quite a promising way to solve the lower mechanical property problem of green compact in the compaction process, although it has some obstacles to overcome for its various applications. Our model proposes that the resultant density is achieved as the applied and resistance forces reach the equilibrium state. Based on the proposed model, the ultrasonic compaction increases the density of green compact by reducing the internal friction between the powder and compaction die, as well as the internal friction among particles themselves. It was also found that during the powder compaction, the ultrasonic vibration mostly contributes to slipping and the rearrangement of the particles.
Monodisperse Fe3O4/SiO2 and Fe3O4/SiO2/PPy Core-Shell Composite Nanospheres for IBU Loading and Release
Authors: Shen Lazhen , Li Bei , Qiao Yongsheng , Song Jinping
Published: March 2019

The magnetic targeting drug delivery system is an effective way of targeting therapy. In this study, the monodisperse Fe3O4 nanoparticles with a particles size of about 180 nm were first prepared via a solvothermal method. Subsequently, the core-shell structure Fe3O4/SiO2 and Fe3O4/SiO2/polypyrrole (PPy) composite nanospheres were successfully synthesized by coating Fe3O4 nanoparticles with SiO2 shell layer using the Stöber method and PPy shell by solvothermal method in turn. The as-prepared nanoparticles were characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FT-IR), vibrating sample magnetometer (VSM), thermogravimetric analysis (TGA), and Ultraviolet-Visible spectrophotometer (UV-Vis). The results indicated that the as-prepared composite nanospheres displayed a well-defined core-shell structure and monodispersity. The thicknesses of SiO2 shell and PPy shell were ~6 nm and ~19 nm, respectively. Additionally, the as-prepared nanoparticles exhibited high saturation magnetization of 104 emu/g, 77 emu/g, and 24 emu/g, and have great potential applications in drug delivery. The drug loading and drug release of the Fe3O4/SiO2 and Fe3O4/SiO2/PPy composite nanospheres to ibuprofen (IBU) under stirring and ultrasonication were investigated. Their drug loading efficiency and drug release efficiency under ultrasonication were all higher than 33% and 90%, respectively. The drug release analyses showed sustained release of IBU from nanospheres and followed the Korsmeyer-Peppas model.
A Theoretical Rigid Body Model of Vibrating Screen for Spring Failure Diagnosis
Authors: Liu Yue , Suo Shuangfu , Meng Guoying , Shang Deyong , Bai Long , Shi Jianwen
Published: March 2019

Springs are critical components in mining vibrating screen elastic supports. However, long-term alternating loads are likely to lead to spring failures, likely resulting in structural damages to the vibrating screen and resulting in a lower separation efficiency. Proper dynamic models provide a basis for spring failure diagnosis. In this paper, a six-degree-of-freedom theoretical rigid body model of a mining vibrating screen is proposed, and a dynamic equation is established in order to explore the dynamic characteristics. Numerical simulations, based on the Newmark-β algorithm, are carried out, and the results indicate that the model proposed is suitable for revealing the dynamic characteristics of the mining vibrating screen. Meanwhile, the mining vibrating screen amplitudes change with the spring failures. Therefore, six types of spring failure are selected for simulations, and the results indicate that the spring failures lead to an amplitude change for the four elastic support points in the x, y, and z directions, where the changes depend on certain spring failures. Hence, the key to spring failure diagnosis lies in obtaining the amplitude change rules, which can reveal particular spring failures. The conclusions provide a theoretical basis for further study and experiments in spring failure diagnosis for a mining vibrating screen.
On (α,β)-US Sets in BCK/BCI-Algebras
Authors: Jana Chiranjibe , Pal Madhumangal
Published: March 2019

Molodtsov originated soft set theory, which followed a general mathematical framework for handling uncertainties, in which we encounter the data by affixing the parameterized factor during the information analysis. The aim of this paper is to establish a bridge to connect a soft set and the union operations on sets, then applying it to B C K / B C I -algebras. Firstly, we introduce the notion of the ( α , β ) -Union-Soft ( ( α , β ) -US) set, with some supporting examples. Then, we discuss the soft B C K / B C I -algebras, which are called ( α , β ) -US algebras, ( α , β ) -US ideals, ( α , β ) -US closed ideals, and ( α , β ) -US commutative ideals. In particular, some related properties and relationships of the above algebraic structures are investigated. We also provide the condition of an ( α , β ) -US ideal to be an ( α , β ) -US closed ideal. Some conditions for a Union-Soft (US) ideal to be a US commutative ideal are given by means of ( α , β ) -unions. Moreover, several characterization theorems of (closed) US ideals and US commutative ideals are given in terms of ( α , β ) -unions. Finally, the extension property for an ( α , β ) -US commutative ideal is established.
Tribochemical Behavior of Pure Magnesium During Sliding Friction
Authors: Zhou Yan , Peng Jinfang , Wang Mengjie , Mo Jiliang , Deng Changguang , Zhu Minhao
Published: March 2019

Reciprocating sliding friction tests were conducted on pure magnesium using a UMT-II tester. The tribo-chemical behavior was characterized using X-ray photoelectron spectroscopy (XPS) and electron probe micro-analyzer (EPMA), which showed that the tribo-chemical behavior of pure magnesium was due to a tribo-oxidation reaction. At room temperature, the debris layer on the worn surface contained Mg(OH)2, MgO, and MgCO3. According to the reciprocating sliding friction mechanism, the decomposition of MgCO3 into MgO should occur. XPS results revealed that the surface oxide layer, containing Mg(OH)2, and MgO, acted as a third layer to protect the surface. Apparently, Mg(OH)2·nH2O was the main tribo-chemical product of pure magnesium under sliding friction.
A Microfluidic Split-Flow Technology for Product Characterization in Continuous Low-Volume Nanoparticle Synthesis
Authors: Bolze Holger , Erfle Peer , Riewe Juliane , Bunjes Heike , Dietzel Andreas , Burg Thomas P.
Published: March 2019

A key aspect of microfluidic processes is their ability to perform chemical reactions in small volumes under continuous flow. However, a continuous process requires stable reagent flow over a prolonged period. This can be challenging in microfluidic systems, as bubbles or particles easily block or alter the flow. Online analysis of the product stream can alleviate this problem by providing a feedback signal. When this signal exceeds a pre-defined range, the process can be re-adjusted or interrupted to prevent contamination. Here we demonstrate the feasibility of this concept by implementing a microfluidic detector downstream of a segmented-flow system for the synthesis of lipid nanoparticles. To match the flow rate through the detector to the measurement bandwidth independent of the synthesis requirements, a small stream is sidelined from the original product stream and routed through a measuring channel with 2 × 2 µm cross-section. The small size of the measuring channel prevents the entry of air plugs, which are inherent to our segmented flow synthesis device. Nanoparticles passing through the small channel were detected and characterized by quantitative fluorescence measurements. With this setup, we were able to count single nanoparticles. This way, we were able to detect changes in the particle synthesis affecting the size, concentration, or velocity of the particles in suspension. We envision that the flow-splitting scheme demonstrated here can be transferred to detection methods other than fluorescence for continuous monitoring and feedback control of microfluidic nanoparticle synthesis.
Development and Validation of a Smartphone-Based Near-Infrared Optical Imaging Device to Measure Physiological Changes In-Vivo
Authors: Kaile Kacie , Godavarty Anuradha
Published: March 2019

Smartphone-based technologies for medical imaging purposes are limited, especially when it involves the measurement of physiological information of the tissues. Herein, a smartphone-based near-infrared (NIR) imaging device was developed to measure physiological changes in tissues across a wide area and without contact. A custom attachment containing multiple multi-wavelength LED light sources (690, 800, and 840 nm; and <4 mW of optical power per LED), source driver, and optical filters and lenses was clipped onto a smartphone that served as the detector during data acquisition. The ability of the device to measure physiological changes was validated via occlusion studies on control subjects. Noise removal techniques using singular value decomposition algorithms effectively removed surface noise and distinctly differentiated the physiological changes in response to occlusion. In the long term, the developed smartphone-based NIR imaging device with capabilities to capture physiological changes will be a great low-cost alternative for clinicians and eventually for patients with chronic ulcers and bed sores, and/or in pre-screening for potential ulcers in diabetic subjects.
Corrosion Behavior of a Pyrite and Arsenopyrite Galvanic Pair in the Presence of Sulfuric Acid, Ferric Ions and HQ0211 Bacterial Strain
Authors: Xu Jia-Ning , Shi Wen-Ge , Ma Peng-Cheng , Lu Liang-Shan , Chen Gui-Min , Yang Hong-Ying
Published: March 2019

In this paper, the galvanic effect of pyrite and arsenopyrite during the leaching pretreatment of gold ores was determined with the use of electrochemical testing (open circuit potential, linear sweep voltammetry, Tafel, and electrochemical impedance spectroscopy (EIS)) and frontier orbit calculations. The results show that (i) the linear sweep voltammetry curve and Tafel curve of the galvanic pair are similar to those of arsenopyrite, (ii) the corrosion behavior of the galvanic pair is consistent with that of arsenopyrite, and (iii) the galvanic effect promotes the corrosion of arsenopyrite by simultaneously increasing the cathode and anode currents and reducing oxidation resistance. The frontier orbit calculation explains the principle of the galvanic effect of pyrite and arsenopyrite from the view of quantum mechanics.
Synthesis and In Vitro Evaluation of Caffeoylquinic Acid Derivatives as Potential Hypolipidemic Agents
Authors: Tian Yu , Cao Xiao-Xue , Shang Hai , Wu Chong-Ming , Zhang Xi , Guo Peng , Zhang Xiao-Po , Xu Xu-Dong
Published: March 2019

A series of novel caffeoylquinic acid derivatives of chlorogenic acid have been designed and synthesized. Biological evaluation indicated that several synthesized derivatives exhibited moderate to good lipid-lowering effects on oleic acid-elicited lipid accumulation in HepG2 liver cells. Particularly, derivatives 3d, 3g, 4c and 4d exhibited more potential lipid-lowering effect than the positive control simvastatin and chlorogenic acid. Further studies on the mechanism of 3d, 3g, 4c and 4d revealed that the lipid-lowering effects were related to their regulation of TG levels and merit further investigation.
Practical Microwave Synthesis of Carbazole Aldehydes for the Development of DNA-Binding Ligands
Authors: Głuszyńska Agata , Juskowiak Bernard
Published: March 2019

Microwave formylation of carbazole derivatives was investigated and 3-monoaldehydes were obtained in high yield. A potential DNA-binding ligand, 3-[(3-ethyl)-2-vinylbenzothiazolium]-9-N-ethyl carbazole iodide, was synthesized and characterized including spectral properties (UV-Vis absorption and fluorescence spectra). The binding selectivity and affinity of three carbazole ligands for double-stranded and G-quadruplex DNA structures were studied using a competitive dialysis method in sodium- and potassium-containing buffer solutions.
Identification and Characterization of the Caspase-Mediated Apoptotic Activity of Teucrium mascatense and an Isolated Compound in Human Cancer Cells
Authors: Panicker Neena Gopinathan , Balhamar Sameera Omar Mohammed Saeed , Akhlaq Shaima , Qureshi Mohammed Mansour , Rizvi Tania Shamim , Al-Harrasi Ahmed , Hussain Javid , Mustafa Farah
Published: March 2019

Plants of the genus Teucrium (Lamiaceae or Labiatae family) are known historically for their medicinal value. Here, we identify and characterize the anticancer potential of T. mascatense and its active compound, IM60, in human cancer cells. The anti-proliferative effect of a T. mascatense methanol extract and its various fractions were analyzed in MCF-7 and HeLa cells in a dose- and time dependent manner. The dichloromethane fraction (TMDF) was observed to be the most effective with cytotoxicity against a more expanded series of cell lines, including MDA-MB-231. A time and dose-dependent toxicity profile was also observed for IM60; it could induce rapid cell death (within 3 h) in MCF-7 cells. Activation of caspases and PARP, hallmarks of apoptotic cell death pathways, following treatment with TMDF was demonstrated using western blot analysis. Inversion of the phosphatidylserine phospholipid from the inner to the outer membrane was confirmed by annexin V staining that was inhibited by the classical apoptosis inhibitor, Z-VAK-FMK. Changes in cell rounding, shrinkage, and detachment from other cells following treatment with TMDF and IM60 also supported these findings. Finally, the potential of TMDF and IM60 to induce enzymatic activity of caspases was also demonstrated in MCF-7 cells. This study, thus, not only characterizes the anticancer potential of T. mascatense, but also identifies a lead terpenoid, IM60, with the potential to activate anticancer cell death pathways in human cancer cells.
Synthesis of New Indanyl Nucleoside Analogues and their Biological Evaluation on Hepatitis C Virus (HCV) Replicon
Authors: Gómez Matías E. , Gentile Emiliano A. , Martini M. Florencia , Cuestas María L. , Mathet Verónica L. , Moltrasio Graciela Y. , Moglioni Albertina G.
Published: March 2019

Here, we report a convenient synthetic procedure for the preparation of four novel indanyl carbanucleoside derivatives in the racemic form. The action of these compounds against hepatitis C virus was evaluated in vitro using the replicon cell line, Huh7.5 SG. Contrary to our expectations, all these compounds did not inhibit, but rather promoted HCV genotype 1b (HCVg1b) replication. Similar effects have been reported for morphine in the replicon cell lines, Huh7 and Huh8. Several biological experiments and computational studies were performed to elucidate the effect of these compounds on HCVg1b replication. Based on all the experiments performed, we propose that the increase in HCVg1b replication could be mediated, at least in part, by a similar mechanism to that of morphine on the enhancement of this replication. The presence of opioid receptors in Huh7.5 SG cells was indirectly determined for the first time in this work.
To Beep or Not to Beep? Evaluating Modalities for Multimodal ICU Alarms
Authors: Cobus Vanessa , Heuten Wilko
Published: March 2019

Technology plays a prominent role in intensive care units (ICU), with a variety of sensors monitoring both patients and devices. A serious problem exists, however, that can reduce the sensors’ effectiveness. When important values exceed or fall below a certain threshold or sensors lose their signal, up to 350 alarms per patient a day are issued. These frequent alarms are audible in several locations on the ICU, resulting in a massive cognitive load for ICU nurses, as they must evaluate and acknowledge each alarm. “Alarm fatigue” sets in, a desensitization and delayed response time for alarms that can have severe consequences for patients and nurses. To counteract the acoustic load on ICUs, we designed and evaluated personal multimodal alarms for a wearable alarm system (WAS). The result was a lower response time and higher ratings on suitability and feasibility, as well as a lower annoyance level, compared to acoustic alarms. We find that multimodal alarms are a promising new approach to alert ICU nurses, reduce cognitive load, and avoid alarm fatigue.
CuInTe2 Nanocrystals: Shape and Size Control, Formation Mechanism and Application, and Use as Photovoltaics
Authors: Jia Guanwei , Liu Baokun , Wang Kun , Wang Chengduo , Yang Peixu , Liu Jinhui , Zhang Weidong , Li Rongbin , Zhang Shaojun , Du Jiang
Published: March 2019

We report on the synthesis of CuInTe2 nanoparticles and their function in photovoltaic equipment, such as solar cells. Under certain synthesis conditions, the CuInTe2 nanocrystals form shape with nanocrystals, nanorods or nanocubes. It was found that CuTe nanocrystals could be converted to CuInTe2 by addition of an In reactant. CuInTe2 nanorods were synthesized using this method.
Impact of the Regularization Parameter in the Mean Free Path Reconstruction Method: Nanoscale Heat Transport and Beyond
Authors: Sanchez-Martinez Miguel-Ángel , Alzina Francesc , Oyarzo Juan , Sotomayor Torres Clivia M. , Chavez-Angel Emigdio
Published: March 2019

The understanding of the mean free path (MFP) distribution of the energy carriers in materials (e.g., electrons, phonons, magnons, etc.) provides a key physical insight into their transport properties. In this context, MFP spectroscopy has become an important tool to describe the contribution of carriers with different MFP to the total transport phenomenon. In this work, we revise the MFP reconstruction technique and present a study on the impact of the regularization parameter on the MFP distribution of the energy carriers. By using the L-curve criterion, we calculate the optimal mathematical value of the regularization parameter. The effect of the change from the optimal value in the MFP distribution is analyzed in three case studies of heat transport by phonons. These results demonstrate that the choice of the regularization parameter has a large impact on the physical information obtained from the reconstructed accumulation function, and thus cannot be chosen arbitrarily. The approach can be applied to various transport phenomena at the nanoscale involving carriers of different physical nature and behavior.
The Impact of Movement Behaviors on Bone Health in Elderly with Adequate Nutritional Status: Compositional Data Analysis Depending on the Frailty Status
Authors: Rodríguez-Gómez Irene , Mañas Asier , Losa-Reyna José , Rodríguez-Mañas Leocadio , Chastin Sebastien F.M. , Alegre Luis M. , García-García Francisco J. , Ara Ignacio
Published: March 2019

The aim of this study was to determine the relationship between bone mass (BM) and physical activity (PA) and sedentary behavior (SB) according to frailty status and sex using compositional data analysis. We analyzed 871 older people with an adequate nutritional status. Fried criteria were used to classify by frailty status. Time spent in SB, light intensity PA (LPA) and moderate-to-vigorous intensity PA (MVPA) was assessed from accelerometry for 7 days. BM was determined by dual-energy X-ray absorptiometry (DXA). The combined effect of PA and SB was significantly associated with BM in robust men and women (p ≤ 0.05). In relation to the other behaviors, SB was negatively associated with BM in robust men while BM was positively associated with SB and negatively with LPA and MVPA in robust women. Moreover, LPA also was positively associated with arm BM (p ≤ 0.01). Finally, in pre-frail women, BM was positively associated with MVPA. In our sample, to decrease SB could be a good strategy to improve BM in robust men. In contrast, in pre-frail women, MVPA may be an important factor to consider regarding bone health.
Clustering of Metabolic Risk Components and Associated Lifestyle Factors: A Nationwide Adolescent Study in Taiwan
Authors: Lin Wei-Ting , Lee Chun-Ying , Tsai Sharon , Huang Hsiao-Ling , Wu Pei-Wen , Chin Yu-Ting , Seal David W. , Chen Ted , Chao Yu-Ying , Lee Chien-Hung
Published: March 2019

Clustering of metabolic syndrome (MetS) risk components in childhood has been linked to a higher risk of diabetes and cardiovascular diseases in adulthood. By using data from the 2010–2011 Nutrition and Health Survey in Taiwan, this study investigated epidemic patterns and correlates for the clustering of MetS risk components. A total of 1920 adolescents aged 12–18 years were included in this study. The MetS diagnostic criteria defined by the Taiwan Pediatric Association (TPA) and International Diabetes Federation (IDF) for adolescents and the criteria defined by the Joint Interim Statement for adults (JIS-Adult) were used to evaluate MetS and its abnormal components. The prevalence of TPA-, IDF-, and JIS-Adult-defined MetS was 4.1%, 3.0%, and 4.0%, with 22.1%, 19.3%, and 17.7%–18.1% of adolescents having high fasting glucose, low high-density lipoprotein cholesterol, and central obesity, respectively. A 0.4-to-0.5-fold decreased risk of having ≥2 MetS abnormal components was detected among adolescents who consumed ≥1 serving/week of dairy products and fresh fruits. Boys who consumed ≥7 drinks/week of soda and girls who consumed ≥7 drinks/week of tea had a 4.6- and 5.2-fold risk of MetS, respectively. In conclusion, our findings revealed significant dimensions of adolescent MetS, including detecting population-specific prevalent patterns for MetS risk components and their clustering, and emphasized on health promotion activities that reduce sugar-sweetened beverage intake.
Gluten Rhapsody
Authors: Elli Luca , Marinoni Beatrice
Published: March 2019

For decades, gluten-free dieting (GFD) has been accepted as the only therapeutic approach to coeliac disease (CD) and, more recently, for non-coeliac gluten sensitivity (NCGS), a term to refer to the so-called gluten-related disorders (GRD) [...]
A Pilot Randomised Controlled Trial of a Text Messaging Intervention with Customisation Using Linked Data from Wireless Wearable Activity Monitors to Improve Risk Factors Following Gestational Diabetes
Authors: Cheung N. Wah , Blumenthal Caron , Smith Ben J. , Hogan Roslyn , Thiagalingam Aravinda , Redfern Julie , Barry Tony , Cinnadaio Nancy , Chow Clara K.
Published: March 2019

Gestational diabetes (GDM) is a highly prevalent disorder of pregnancy which portends a high risk for future type 2 diabetes. Limited evidence indicates lifestyle intervention prevents the development of diabetes, but most previously studied interventions are resource-intensive. Intervention programs that utilise newer technologies may be scalable at lower cost. This 6-month pilot randomized controlled trial tested the delivery of text messages linked to an activity monitor, adaptive physical activity goal setting, and limited face-to-face counseling, as an intervention to improve rates of post-partum glucose tolerance testing and lifestyle behaviours amongst women following a GDM pregnancy. Sixty subjects were randomised 2:1 intervention vs. control. Compared to control subjects, there were trends for intervention subjects to improve diet, increase physical activity, and lose weight. There was no difference between the groups in the rate of glucose tolerance testing. Only 46 (77%) subjects completed some, and 19 subjects completed all the elements of the final evaluation. Feedback regarding the text messages and activity monitor was highly positive. Overall, results suggest that a text message and activity monitor intervention is feasible for a larger study or even as a potentially scalable population health intervention. However, low completion rates necessitate carefully considered modification of the protocol.
Drug Solubility Enhancement through the Preparation of Multicomponent Organic Materials: Eutectics of Lovastatin with Carboxylic Acids
Authors: Araya-Sibaja Andrea Mariela , Vega-Baudrit José Roberto , Guillén-Girón Teodolito , Navarro-Hoyos Mirtha , Cuffini Silvia Lucia
Published: March 2019

Lovastatin (LOV) is a drug used to treat hypercholesterolemia. Recent studies have identified its antioxidant effects and potential use in the treatment of some types of cancer. However, the low bioavailability related to its poor water solubility limits its use in solid oral dosage forms. Therefore, to improve the solubility of LOV three eutectic systems of LOV with the carboxylic acids benzoic (BEN), salicylic (SAL) and cinnamic (CIN) were obtained. Both binary phase and Tammann diagrams were constructed using differential scanning calorimetry (DSC) data of mixtures prepared from 0.1 to 1.0 molar ratios. Binary mixtures and eutectics were prepared by liquid-assisted grinding. The eutectics were further characterized by DSC and powder X-ray diffraction (PXRD), Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The LOV-BEN, LOV-SAL and LOV-CIN system formed a eutectic at an LOV mole fraction of 0.19, 0.60 and 0.14, respectively. The systems exhibited improvements in LOV solubility, becoming more soluble by five-fold in the LOV-SAL system and approximately four-fold in the other two systems. Considering that the solubility enhancements and the carboxylic acids used are generally recognized as safe by the U.S. Food and Drug Administration (FDA), the LOV eutectic systems are promising materials to be used in a solubility enhancement strategy for pharmaceutical product formulation.
Gene Therapy Approaches to Functional Cure and Protection of Hematopoietic Potential in HIV Infection
Authors: Tsukamoto Tetsuo
Published: March 2019

Although current antiretroviral drug therapy can suppress the replication of human immunodeficiency virus (HIV), a lifelong prescription is necessary to avoid viral rebound. The problem of persistent and ineradicable viral reservoirs in HIV-infected people continues to be a global threat. In addition, some HIV-infected patients do not experience sufficient T-cell immune restoration despite being aviremic during treatment. This is likely due to altered hematopoietic potential. To achieve the global eradication of HIV disease, a cure is needed. To this end, tremendous efforts have been made in the field of anti-HIV gene therapy. This review will discuss the concepts of HIV cure and relative viral attenuation and provide an overview of various gene therapy approaches aimed at a complete or functional HIV cure and protection of hematopoietic functions.