bluuebunny/crossref_metadata_2025 huggingface dataset:
Facet-Dependent Reactivity of Ceria Nanoparticles Exemplified by CeO2-Based Transition Metal Catalysts: A Critical Review
Authors: Konsolakis Michalis , Lykaki Maria
Published: March 2021

The rational design and fabrication of highly-active and cost-efficient catalytic materials constitutes the main research pillar in catalysis field. In this context, the fine-tuning of size and shape at the nanometer scale can exert an intense impact not only on the inherent reactivity of catalyst’s counterparts but also on their interfacial interactions; it can also opening up new horizons for the development of highly active and robust materials. The present critical review, focusing mainly on our recent advances on the topic, aims to highlight the pivotal role of shape engineering in catalysis, exemplified by noble metal-free, CeO2-based transition metal catalysts (TMs/CeO2). The underlying mechanism of facet-dependent reactivity is initially discussed. The main implications of ceria nanoparticles’ shape engineering (rods, cubes, and polyhedra) in catalysis are next discussed, on the ground of some of the most pertinent heterogeneous reactions, such as CO2 hydrogenation, CO oxidation, and N2O decomposition. It is clearly revealed that shape functionalization can remarkably affect the intrinsic features and in turn the reactivity of ceria nanoparticles. More importantly, by combining ceria nanoparticles (CeO2 NPs) of specific architecture with various transition metals (e.g., Cu, Fe, Co, and Ni) remarkably active multifunctional composites can be obtained due mainly to the synergistic metalceria interactions. From the practical point of view, novel catalyst formulations with similar or even superior reactivity to that of noble metals can be obtained by co-adjusting the shape and composition of mixed oxides, such as Cu/ceria nanorods for CO oxidation and Ni/ceria nanorods for CO2 hydrogenation. The conclusions derived could provide the design principles of earth-abundant metal oxide catalysts for various real-life environmental and energy applications.
MUSE Stem Cells Can Be Isolated from Stromal Compartment of Mouse Bone Marrow, Adipose Tissue, and Ear Connective Tissue: A Comparative Study of Their In Vitro Properties
Authors: Aprile Domenico , Alessio Nicola , Demirsoy Ibrahim H. , Squillaro Tiziana , Peluso Gianfranco , Di Bernardo Giovanni , Galderisi Umberto
Published: March 2021

The cells present in the stromal compartment of many tissues are a heterogeneous population containing stem cells, progenitor cells, fibroblasts, and other stromal cells. A SSEA3(+) cell subpopulation isolated from human stromal compartments showed stem cell properties. These cells, known as multilineage-differentiating stress-enduring (MUSE) cells, are capable of resisting stress and possess an excellent ability to repair DNA damage. We isolated MUSE cells from different mouse stromal compartments, such as those present in bone marrow, subcutaneous white adipose tissue, and ear connective tissue. These cells showed overlapping in vitro biological properties. The mouse MUSE cells were positive for stemness markers such as SOX2, OCT3/4, and NANOG. They also expressed TERT, the catalytic telomerase subunit. The mouse MUSE cells showed spontaneous commitment to differentiation in meso/ecto/endodermal derivatives. The demonstration that multilineage stem cells can be isolated from an animal model, such as the mouse, could offer a valid alternative to the use of other stem cells for disease studies and envisage of cellular therapies.
The Metabolic Fates of Pyruvate in Normal and Neoplastic Cells
Authors: Prochownik Edward V. , Wang Huabo
Published: March 2021

Pyruvate occupies a central metabolic node by virtue of its position at the crossroads of glycolysis and the tricarboxylic acid (TCA) cycle and its production and fate being governed by numerous cell-intrinsic and extrinsic factors. The former includes the cell’s type, redox state, ATP content, metabolic requirements and the activities of other metabolic pathways. The latter include the extracellular oxygen concentration, pH and nutrient levels, which are in turn governed by the vascular supply. Within this context, we discuss the six pathways that influence pyruvate content and utilization: 1. The lactate dehydrogenase pathway that either converts excess pyruvate to lactate or that regenerates pyruvate from lactate for use as a fuel or biosynthetic substrate; 2. The alanine pathway that generates alanine and other amino acids; 3. The pyruvate dehydrogenase complex pathway that provides acetyl-CoA, the TCA cycle’s initial substrate; 4. The pyruvate carboxylase reaction that anaplerotically supplies oxaloacetate; 5. The malic enzyme pathway that also links glycolysis and the TCA cycle and generates NADPH to support lipid bio-synthesis; and 6. The acetate bio-synthetic pathway that converts pyruvate directly to acetate. The review discusses the mechanisms controlling these pathways, how they cross-talk and how they cooperate and are regulated to maximize growth and achieve metabolic and energetic harmony.
Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles Elicit Better Preservation of the Intra-Renal Microvasculature Than Renal Revascularization in Pigs with Renovascular Disease
Authors: Ferguson Christopher M. , Farahani Rahele A. , Zhu Xiang-Yang , Tang Hui , Jordan Kyra L. , Saadiq Ishran M. , Lerman Amir , Lerman Lilach O. , Eirin Alfonso
Published: March 2021

Background: Percutaneous transluminal renal angioplasty (PTRA) confers clinical and mortality benefits in select ‘high-risk’ patients with renovascular disease (RVD). Intra-renal-delivered extracellular vesicles (EVs) released from mesenchymal stem/stromal cells (MSCs) protect the kidney in experimental RVD, but have not been compared side-by-side to clinically applied interventions, such as PTRA. We hypothesized that MSC-derived EVs can comparably protect the post-stenotic kidney via direct tissue effects. Methods: Five groups of pigs (n = 6 each) were studied after 16 weeks of RVD, RVD treated 4 weeks earlier with either PTRA or MSC-derived EVs, and normal controls. Single-kidney renal blood flow (RBF) and glomerular filtration rate (GFR) were assessed in vivo with multi-detector CT, and renal microvascular architecture (3D micro CT) and injury pathways ex vivo. Results: Despite sustained hypertension, EVs conferred greater improvement of intra-renal microvascular and peritubular capillary density compared to PTRA, associated with attenuation of renal inflammation, oxidative stress, and tubulo-interstitial fibrosis. Nevertheless, stenotic kidney RBF and GFR similarly rose in both PTRA- and EV-treated pigs compared RVD + Sham. mRNA sequencing reveled that EVs were enriched with pro-angiogenic, anti-inflammatory, and antioxidants genes. Conclusion: MSC-derived EVs elicit a better preservation of the stenotic kidney microvasculature and greater attenuation of renal injury and fibrosis compared to PTRA, possibly partly attributed to their cargo of vasculo-protective genes. Yet, both strategies similarly improve renal hemodynamics and function. These observations shed light on diverse mechanisms implicated in improvement of post-stenotic kidney function and position EVs as a promising therapeutic intervention in RVD.
High-Density Lipoproteins and the Kidney
Authors: Strazzella Arianna , Ossoli Alice , Calabresi Laura
Published: March 2021

Dyslipidemia is a typical trait of patients with chronic kidney disease (CKD) and it is typically characterized by reduced high-density lipoprotein (HDL)-cholesterol(c) levels. The low HDL-c concentration is the only lipid alteration associated with the progression of renal disease in mild-to-moderate CKD patients. Plasma HDL levels are not only reduced but also characterized by alterations in composition and structure, which are responsible for the loss of atheroprotective functions, like the ability to promote cholesterol efflux from peripheral cells and antioxidant and anti-inflammatory proprieties. The interconnection between HDL and renal function is confirmed by the fact that genetic HDL defects can lead to kidney disease; in fact, mutations in apoA-I, apoE, apoL, and lecithin–cholesterol acyltransferase (LCAT) are associated with the development of renal damage. Genetic LCAT deficiency is the most emblematic case and represents a unique tool to evaluate the impact of alterations in the HDL system on the progression of renal disease. Lipid abnormalities detected in LCAT-deficient carriers mirror the ones observed in CKD patients, which indeed present an acquired LCAT deficiency. In this context, circulating LCAT levels predict CKD progression in individuals at early stages of renal dysfunction and in the general population. This review summarizes the main alterations of HDL in CKD, focusing on the latest update of acquired and genetic LCAT defects associated with the progression of renal disease.
Comparison of Biocompatible Coatings Produced by Plasma Electrolytic Oxidation on cp-Ti and Ti-Zr-Nb Superelastic Alloy
Authors: Farrakhov Ruzil , Melnichuk Olga , Parfenov Evgeny , Mukaeva Veta , Raab Arseniy , Sheremetyev Vadim , Zhukova Yulia , Prokoshkin Sergey
Published: March 2021

The paper compares the coatings produced by plasma electrolytic oxidation (PEO) on commercially pure titanium and a novel superelastic alloy Ti-18Zr-15Nb (at. %) for implant applications. The PEO coatings were produced on both alloys in the identical pulsed bipolar regime. The properties of the coatings were examined using scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDX), potentiodynamic polarization (PDP), and electrochemical impedance spectroscopy (EIS). The PEO process kinetics was modeled based on the Avrami theorem and Cottrell equation using a relaxation method. The resultant coatings contain TiO2, for both alloys, and NbO2, Nb2O5, ZrO2 for Ti-18Zr-15Nb alloy. The coating on the Ti-18Zr-15Nb alloy has a higher thickness, porosity, and roughness compared to that on cp-Ti. The values of the kinetic coefficients of the PEO process—higher diffusion coefficient and lower time constant for the processing of Ti-18Zr-15Nb—explain this effect. According to the electrochemical studies, PEO coatings on Ti-18Zr-15Nb alloy provide better corrosion protection. Higher corrosion resistance, porosity, and roughness contribute to better biocompatibility of the PEO coating on Ti-18Zr-15Nb alloy compared to cp-Ti.
Conservation Genetics of Four Critically Endangered Greek Endemic Plants: A Preliminary Assessment
Authors: Kougioumoutzis Konstantinos , Kotsakiozi Panayiota , Stathi Efthalia , Trigas Panayiotis , Parmakelis Aristeidis
Published: March 2021

The Mediterranean basin constitutes one of the largest global biodiversity hotspots, hosting more than 11,000 endemic plants, and it is recognised as an area with a high proportion of threatened taxa. Nevertheless, only a tiny fraction of the threatened Mediterranean endemics have their genetic diversity assessed, and we are unaware if and how climate change might impact their conservation status. This is even more pronounced in Eastern Mediterranean countries with a rich endemic flora, such as Greece, which hosts a large portion of the plant taxa assessed at the European level under the IUCN criteria. Using inter simple sequence repeats (ISSR) markers and species distribution models, we analysed the genetic diversity and investigated the impacts of climate change on four critically endangered and extremely narrow and rare Greek island endemic plants, namely Aethionema retsina, Allium iatrouinum, Convolvulus argyrothamnos, and Saponaria jagelii. All four species are facing intense anthropogenic threats and display moderate genetic diversity (uHe: 0.254–0.322), while climate change is expected to have a profound impact on their range size during the coming decades. A combination of in- and ex-situ measures, such as population reinforcement and seed bank conservation, are urgently needed in order to preserve these highly threatened and rare Greek endemics.
Comparison of Regularized Reconstruction and Ordered Subset Expectation Maximization Reconstruction in the Diagnostics of Prostate Cancer Using Digital Time-of-Flight 68Ga-PSMA-11 PET/CT Imaging
Authors: Jonmarker Olof , Axelsson Rimma , Nilsson Ted , Gabrielson Stefan
Published: March 2021

In prostate cancer, the early detection of distant spread has been shown to be of importance. Prostate-specific membrane antigen (PSMA)-binding radionuclides in positron emission tomography (PET) is a promising method for precise disease staging. PET diagnostics depend on image reconstruction techniques, and ordered subset expectation maximization (OSEM) is the established standard. Block sequential regularized expectation maximization (BSREM) is a more recent reconstruction algorithm and may produce fewer equivocal findings and better lesion detection. Methods: 68Ga PSMA-11 PET/CT scans of patients with de novo or suspected recurrent prostate cancer were retrospectively reformatted using both the OSEM and BSREM algorithms. The lesions were counted and categorized by three radiologists. The intra-class correlation (ICC) and Cohen’s kappa for the inter-rater reliability were calculated. Results: Sixty-one patients were reviewed. BSREM identified slightly fewer lesions overall and fewer equivocal findings. ICC was excellent with regards to definitive lymph nodes and bone metastasis identification and poor with regards to equivocal metastasis irrespective of the reconstruction algorithm. The median Cohen’s kappa were 0.66, 0.74, 0.61 and 0.43 for OSEM and 0.61, 0.63, 0.66 and 0.53 for BSREM, with respect to the tumor, local lymph nodes, metastatic lymph nodes and bone metastasis detection, respectively. Conclusions: BSREM in the setting of 68Ga PMSA PET staging or restaging is comparable to OSEM.
My Family Stands Behind Me: Moderated Mediation Model of Family Support and Work Engagement
Authors: Erum Humaira , Abid Ghulam , Anwar Aizza , Ijaz Muhammad Fazal , Kee Daisy Mui Hung
Published: March 2021

Family motivation as a mediating mechanism is a novel and under-researched area in the field of positive organizational scholarship. Drawing on Social Exchange Theory (SET), this study empirically validates family motivation as a mediator between family support and work engagement. The process by Hayes (2013) was used to analyze time-lagged data collected from 356 employees of the education sector. Results confirm the mediating role of family motivation in the relationship between family support and work engagement and the moderating role of calling in the relationship between family support and family motivation. This study adds to the literature of family-work enrichment accounts by validating family support as a novel antecedent for family motivation and positive attitudes. The implications of the study are discussed.
Semi-Automatic Guidance vs. Manual Guidance in Agriculture: A Comparison of Work Performance in Wheat Sowing
Authors: Scarfone Antonio , Picchio Rodolfo , del Giudice Angelo , Latterini Francesco , Mattei Paolo , Santangelo Enrico , Assirelli Alberto
Published: March 2021

The use of digital systems in precision agriculture is becoming more and more attractive for farmers at every level. A few years ago, the use of these technologies was limited to large farms, due to the considerable income needed to amortize the large investment required. Although this technology has now become more affordable, there is a lack of scientific data directed to demonstrate how these systems are able to determine quantifiable advantages for farmers. Thus, the transition towards precision agriculture is still very slow. This issue is not just negatively affecting the agriculture economy, but it is also slowing down potential environmental benefits that may result from it. The starting point of precision agriculture can be considered as the introduction of satellite tractor guidance. For instance, with semi-automatic and automatic tractor guidance, farmers can profit from more accuracy and higher machine performance during several farm operations such as plowing, harrowing, sowing, and fertilising. The goal of this study is to compare semi-automatic guidance with manual guidance in wheat sowing, evaluating parameters such as machine performance, seed supply and operational costs of both the configurations.
Research on Control Strategy of Isolated DC Microgrid Based on SOC of Energy Storage System
Authors: Lv Jiechao , Wang Xiaoli , Wang Guishuo , Song Yuhou
Published: March 2021

With the rapid development of renewable energy technologies, islanded DC microgrids have received extensive attention in the field of distributed power generation due to their plug-and-play, flexible operation modes and convenient power conversion, and are likely to be one of the mainstream structures of microgrids in the future. The islanded DC microgrid contains multiple distributed power generation units. The battery energy storage system (BESS) is the main controlled unit used to smooth power fluctuations. The main parameter of concern is the state of charge (SOC). In order to maintain the stability of the microgrid, this paper takes the islanded DC microgrid as the research object and designs a control strategy based on the SOC of the BESS. Additionally, in the control strategy, the BESS’s energy balance control strategy and the microgrid’s operation control strategy are emphatically designed. The designed BESS control strategy adjusts the droop coefficient in real time according to the SOC of the battery energy storage unit (BESU), and controls the charge and discharge power of the BESU to achieve the SOC balance among the BESUs. The microgrid operation control strategy takes the energy storage system (ESS) as the main controlled unit to suppress power fluctuations, and distributes the power of distributed power sources according to the SOC of the BESS to achieve power balance in the microgrid, and control the DC bus voltage fluctuation deviation within 4.5%.
Age and Gender as Cyber Attribution Features in Keystroke Dynamic-Based User Classification Processes
Authors: Tsimperidis Ioannis , Yucel Cagatay , Katos Vasilios
Published: March 2021

Keystroke dynamics are used to authenticate users, to reveal some of their inherent or acquired characteristics and to assess their mental and physical states. The most common features utilized are the time intervals that the keys remain pressed and the time intervals that are required to use two consecutive keys. This paper examines which of these features are the most important and how utilization of these features can lead to better classification results. To achieve this, an existing dataset consisting of 387 logfiles is used, five classifiers are exploited and users are classified by gender and age. The results, while demonstrating the application of these two characteristics jointly on classifiers with high accuracy, answer the question of which keystroke dynamics features are more appropriate for classification with common classifiers.
Rheological Properties of Wood–Plastic Composites by 3D Numerical Simulations: Different Components
Authors: Lv Xingcong , Hao Xiaolong , Ou Rongxian , Liu Tao , Guo Chuigen , Wang Qingwen , Yi Xin , Sun Lichao
Published: March 2021

The rheological properties of wood–plastic composites (WPCs) with different wood fiber contents were investigated using a rotational rheometer under low shear rates. The flow field information was analyzed and simulated by Ansys Polyflow software. The results showed that the WPCs with different wood fiber contents behaved as typical power-law fluids. A higher wood fiber content increased the shear thinning ability and pseudoplasticity of the WPCs. The pressure, velocity, shear rate, and viscosity distributions of the WPC during extrusion could be predicted by computational fluid dynamics (CFD) Ansys Polyflow software to explore the effects of different components on the flow field of WPCs.
Loss-of-Function Variants in EFEMP1 Cause a Recognizable Connective Tissue Disorder Characterized by Cutis Laxa and Multiple Herniations
Authors: Verlee Maxim , Beyens Aude , Gezdirici Alper , Gulec Elif Yilmaz , Pottie Lore , De Feyter Silke , Vanhooydonck Michiel , Tapaneeyaphan Piyanoot , Symoens Sofie , Callewaert Bert
Published: March 2021

Hereditary disorders of connective tissue (HDCT) compromise a heterogeneous group of diseases caused by pathogenic variants in genes encoding different components of the extracellular matrix and characterized by pleiotropic manifestations, mainly affecting the cutaneous, cardiovascular, and musculoskeletal systems. We report the case of a 9-year-old boy with a discernible connective tissue disorder characterized by cutis laxa (CL) and multiple herniations and caused by biallelic loss-of-function variants in EFEMP1. Hence, we identified EFEMP1 as a novel disease-causing gene in the CL spectrum, differentiating it from other HDCT.
De Novo 1q21.3q22 Duplication Revaluation in a “Cold” Complex Neuropsychiatric Case with Syndromic Intellectual Disability
Authors: Milone Roberta , Scalise Roberta , Pasquariello Rosa , Berloffa Stefano , Ricca Ivana , Battini Roberta
Published: March 2021

Syndromic intellectual disability often obtains a genetic diagnosis due to the combination of first and next generation sequencing techniques, although their interpretation may require revaluation over the years. Here we report on a composite neuropsychiatric case whose phenotype includes moderate intellectual disability, spastic paraparesis, movement disorder, and bipolar disorder, harboring a 1.802 Mb de novo 1q21.3q22 duplication. The role of this duplication has been reconsidered in the light of negativity of many other genetic exams, and of the possible pathogenic role of many genes included in this duplication, potentially configuring a contiguous gene-duplication syndrome.
Heterogeneity of the Clinical Presentation of the MEN1 LRG_509 c.781C>T (p.Leu261Phe) Variant Within a Three-Generation Family
Authors: Gilis-Januszewska Aleksandra , Bogusławska Anna , Hasse-Lazar Kornelia , Jurecka-Lubieniecka Beata , Jarząb Barbara , Sowa-Staszczak Anna , Opalińska Marta , Godlewska Magdalena , Grochowska Anna , Skalniak Anna , Hubalewska-Dydejczyk Alicja
Published: March 2021

Multiple neuroendocrine neoplasia type 1 (MEN1) is a rare genetic disorder with an autosomal dominant inheritance, predisposing carriers to benign and malignant tumors. The phenotype of MEN1 syndrome varies between patients in terms of tumor localization, age of onset, and clinical aggressiveness, even between affected members within the same family. We describe a heterogenic phenotype of the MEN1 variant c.781C>T (LRG_509t1), which was previously reported only once in a family with isolated hyperparathyroidism. A heterozygous missense variant in exon 4 of the gene was identified in the sequence of the MEN1 gene, i.e., c.781C>T, leading to the amino acid change p.Leu261Phe in a three-generation family. In the screened family, 5/6 affected members had already developed hyperparathyroidism. In the index patient and two other family members, an aggressive course of pancreatic neuroendocrine tumor (insulinoma and non-functioning neuroendocrine tumors) with dissemination was diagnosed. In the index patient, late diagnosis and slow progression of the disseminated neuroendocrine tumor have been observed (24 years of follow-up). The very rare variant of MEN1, LRG_509t1 c.781C>T /p.Leu261Phe (LRG_509p1), diagnosed within a three-generation family has a heterogenic clinical presentation. Further follow-up of the family members should be carried out to confirm the spectrum and exact time of clinical presentation.
LOXL4 Abrogation Does Not Exaggerate Angiotensin II-Induced Thoracic or Abdominal Aortic Aneurysm in Mice
Authors: Li Huimin , Guo Jun , Jia Yiting , Kong Wei , Li Wei
Published: March 2021

It has been shown that thoracic aortic aneurysm and dissection (TAAD) could be a Mendelian trait caused by a single gene mutation. The LOX gene mutation leads to the development of human TAAD. The LOXL4 gene is a member of the lysyl oxidase gene family. We identified seven variants in the LOXL4 gene in 219 unrelated patients with TAAD by whole-exome sequencing (WES). To further investigate whether LOXL4 is a candidate causative gene for human TAAD, a LOXL4 knockout mouse was generated, and the mutant mice were treated by subcutaneous infusion of angiotensin II. We found that abrogation of LOXL4 did not induce a more severe thoracic or abdominal aortic aneurysm compared with the wild-type C57BL/6J mice. Our results suggest that LOXL4 may not play a major role in the development of angiotensin II-induced aortic aneurysm. The functional study using this animal model system is important for the evaluation of candidate genes of TAAD identified by WES.
Paving the Way for Outdoor Play: Examining Socio-Environmental Barriers to Community-Based Outdoor Play
Authors: Loebach Janet , Sanches Marcos , Jaffe Julia , Elton-Marshall Tara
Published: March 2021

Outdoor play and independent, neighborhood activity, both linked with healthy childhood development, have declined dramatically among Western children in recent decades. This study examines how social, cultural and environmental factors may be hindering children’s outdoor and community-based play. A comprehensive survey was completed by 826 children (aged 10–13 years) and their parents from 12 schools (four each urban, suburban and rural) from a large county in Ontario, Canada. Five multilevel regression models, controlling for any school clustering effect, examined associations between outdoor play time per week and variable sets representing five prevalent factors cited in the literature as influencing children’s outdoor play (OP). Models predicted that younger children and boys were more likely to spend time playing outdoors; involvement in organized physical activities, other children nearby to play with, higher perception of benefits of outdoor play, and higher parental perceptions of neighborhood social cohesion also predicted more time in outdoor play. Time outdoors was less likely among children not allowed to play beyond home without supervision, felt they were ‘too busy’ with screen-based activities, and who reported higher fears related to playing outdoors. Study findings have important implications for targeting environmental, cultural and policy changes to foster child-friendly communities which effectively support healthy outdoor play.
Symmetry Criterion for Patients with Rheumatoid Arthritis of the Foot: A Cross-Sectional Study
Authors: Sanchez-Castillo Jose Alberto , Reinoso-Cobo Andres , Gijon-Nogueron Gabriel , Caliz-Caliz Rafael , Exposito-Ruiz Manuela , Ramos-Petersen Laura , Ortega-Avila Ana Belen
Published: March 2021

Objective: The aim of the study was to analyze the feet of rheumatoid arthritis (RA) patients, to determine the degree to which both feet were affected, primarily analyzing the severity of RA in both feet looking at structure and morphology, and secondly looking at the symmetry in terms of the anthropometrics and posture. Method: This cross-sectional study was conducted from January to December 2018. The data from 229 patients with RA and with foot pain and no RA recruited (Granada, Spain) were analyzed. Two researchers independently interviewed the patients to obtain the study data. The clinical data were obtained using specific foot health and quality of life questionnaires and a validated platform for foot measurement. Anthropometric measurements were obtained by means of a foot measurement platform and the Foot Posture Index (FPI). The bivariate analysis was performed with the Student’s t test and the non-parametric Wilcoxon test. The level of significance was established at p < 0.05. Results: In the RA group, anthropometric measurements revealed significant differences between the left and right feet in 13 of the 23 parameters considered, as follows: (non-load-bearing) foot length, length of the first metatarsophalangeal joint, maximum height of the internal longitudinal arch, and width of the midfoot (p < 0.001, p = 0.038, p < 0.001, and p = 0.037 respectively); and Foot Posture Index (p = 0.001). Conclusions: In patients with RA, statistically significant differences were found in the Foot Posture Index and in several parameters related to foot structure and morphology. From this, we conclude that from a morphological, structural, and postural standpoint, a pattern of symmetric joint involvement should not be viewed as a specific criterion for RA in the foot.
Health Knowledge about Smoking, Role of Doctors, and Self-Perceived Health: A Cross-Sectional Study on Smokers’ Intentions to Quit
Authors: He Tianfeng , Liu Lefan , Huang Jing , Li Guoxing , Guo Xinbiao
Published: March 2021

Limited empirical work has been done to compare the effects that health knowledge and advice from doctors have on smokers’ intentions to quit. This paper examines the association of smokers’ intentions to quit with health knowledge, advice from doctors, and self-perceived health. A sample of 2509 smokers aged 15–69 years old in Ningbo was used from China’s National Health Literacy Surveillance survey, conducted in 2018 and 2019. Respondents were asked whether they agree smoking causes stroke, heart attack, lung cancer; and heart diseases in adults, lung illnesses in children, and lung cancer in adults, by secondhand smoke, respectively. Using the logistic model, we found that knowing that smoking causes stroke and lung cancer more than doubles the odds of one’s intention to quit (OR = 2.705, p < 0.01), the effect of which is much greater than knowing that smoking causes lung cancer only (OR = 1.795, p < 0.01). Doctors’ advice to quit is more important than health knowledge, in terms of predicting smokers’ past cessation behaviours. In addition, smokers’ self-perceived health is negatively associated with their decisions to quit. This paper highlights that more resources should be directed towards training health care providers to advise smokers to quit, which might be more effective than health education alone.
Discrimination and Violence Due to Diversity of Sexual Orientation and Gender Identity: Explanatory Variables
Authors: Inmaculada Fernández-Antelo , Isabel Cuadrado-Gordillo
Published: March 2021

Knowledge of the processes of aggression and victimization in couple relationships cannot be approached through the treatment of single variables. It needs a multidimensional perspective that establishes a web of relationships between variables of different types. The objectives of the present study were: (i) to explore the interrelationships between and interdependence of empathy, moral disengagement, homophobic attitudes, and prejudice as explanatory variables of discrimination and violence towards couples due to gender issues; and (ii) to delimit predictive indicators of the manifestation of aggressive attitudes and prejudices towards homosexual couples. The sample comprised 778 young people of ages 18 to 24 years (M = 19.9; SD = 1.6). Through the use of four instruments, it was found that empathy is a strong protector against homophobic attitudes, while moral disengagement is a predictor of aggressive attitudes towards same-sex couples. The results make it possible to delimit homophobic profiles and obtain predictive indicators that will be key elements in the design of programs and measures to prevent violence towards couples for reasons of gender.
Effects of 4 Weeks of a Technique-Specific Protocol with High-Intensity Intervals on General and Specific Physical Fitness in Taekwondo Athletes: An Inter-Individual Analysis
Authors: Ojeda-Aravena Alex , Herrera-Valenzuela Tomás , Valdés-Badilla Pablo , Cancino-López Jorge , Zapata-Bastias José , García-García José Manuel
Published: March 2021

The aim of this research was to compare the effects of a technique-specific high-intensity interval training (HIIT) protocol vs. traditional taekwondo training on physical fitness and body composition in taekwondo athletes, as well as to analyse the inter-individual response. Utilising a parallel controlled design, sixteen male and female athletes (five females and 11 males) were randomly divided into an experimental group (EG) that participated in the technique-specific HIIT and a control group (CG) that participated in traditional taekwondo training. Both groups trained three days/week for four weeks. Squat jump (SJ), countermovement jump (CMJ), 5-metre sprint (5M), 20-metre shuttle run (20MSR), taekwondo specific agility test (TSAT), multiple frequency speed of kick test (FSKTMULT), total kicks, and kick decrement index (KDI), as well as body composition were evaluated. Results indicate that there are no significant differences (p > 0.05) in the factors group and time factor and group by time interaction (p > 0.05). Although percentage and effect size increases were documented for post-intervention fitness components in TSAT, total kicks, KDI, and 20MSR, responders and non-responders were also documented. In conclusion, a HIIT protocol based on taekwondo-specific technical movements does not report significant differences in fitness and body composition compared to traditional taekwondo training, nor inter-individual differences between athletes.
Metabolic Interventions to Prevent Hypertrophy-Induced Alterations in Contractile Properties In Vitro
Authors: Geraets Ilvy M. E. , Coumans Will A. , Strzelecka Agnieszka , Schönleitner Patrick , Antoons Gudrun , Schianchi Francesco , Willemars Myrthe M. A. , Kapsokalyvas Dimitrios , Glatz Jan F. C. , Luiken Joost J. F. P. , Nabben Miranda
Published: March 2021

(1) Background: The exact mechanism(s) underlying pathological changes in a heart in transition to hypertrophy and failure are not yet fully understood. However, alterations in cardiac energy metabolism seem to be an important contributor. We characterized an in vitro model of adrenergic stimulation-induced cardiac hypertrophy for studying metabolic, structural, and functional changes over time. Accordingly, we investigated whether metabolic interventions prevent cardiac structural and functional changes; (2) Methods: Primary rat cardiomyocytes were treated with phenylephrine (PE) for 16 h, 24 h, or 48 h, whereafter hypertrophic marker expression, protein synthesis rate, glucose uptake, and contractile function were assessed; (3) Results: 24 h PE treatment increased expression of hypertrophic markers, phosphorylation of hypertrophy-related signaling kinases, protein synthesis, and glucose uptake. Importantly, the increased glucose uptake preceded structural and functional changes, suggesting a causal role for metabolism in the onset of PE-induced hypertrophy. Indeed, PE treatment in the presence of a PAN-Akt inhibitor or of a GLUT4 inhibitor dipyridamole prevented PE-induced increases in cellular glucose uptake and ameliorated PE-induced contractile alterations; (4) Conclusions: Pharmacological interventions, forcing substrate metabolism away from glucose utilization, improved contractile properties in PE-treated cardiomyocytes, suggesting that targeting glucose uptake, independent from protein synthesis, forms a promising strategy to prevent hypertrophy and hypertrophy-induced cardiac dysfunction.
Periostin Is Required for the Maintenance of Muscle Fibers during Muscle Regeneration
Authors: Ito Naoki , Miyagoe-Suzuki Yuko , Takeda Shin’ichi , Kudo Akira
Published: March 2021

Skeletal muscle regeneration is a well-organized process that requires remodeling of the extracellular matrix (ECM). In this study, we revealed the protective role of periostin, a matricellular protein that binds to several ECM proteins during muscle regeneration. In intact muscle, periostin was localized at the neuromuscular junction, muscle spindle, and myotendinous junction, which are connection sites between muscle fibers and nerves or tendons. During muscle regeneration, periostin exhibited robustly increased expression and localization at the interstitial space. Periostin-null mice showed decreased muscle weight due to the loss of muscle fibers during repeated muscle regeneration. Cultured muscle progenitor cells from periostin-null mice showed no deficiencies in their proliferation, differentiation, and the expression of Pax7, MyoD, and myogenin, suggesting that the loss of muscle fibers in periostin-null mice was not due to the impaired function of muscle stem/progenitor cells. Periostin-null mice displayed a decreased number of CD31-positive blood vessels during muscle regeneration, suggesting that the decreased nutritional supply from blood vessels was the cause of muscle fiber loss in periostin-null mice. These results highlight the novel role of periostin in maintaining muscle mass during muscle regeneration.
Computational Analysis of the Interactions between the S100B Extracellular Chaperone and Its Amyloid β Peptide Client
Authors: Rodrigues Filipe E. P. , Figueira António J. , Gomes Cláudio M. , Machuqueiro Miguel
Published: March 2021

S100B is an astrocytic extracellular Ca2+-binding protein implicated in Alzheimer’s disease, whose role as a holdase-type chaperone delaying Aβ42 aggregation and toxicity was recently uncovered. Here, we employ computational biology approaches to dissect the structural details and dynamics of the interaction between S100B and Aβ42. Driven by previous structural data, we used the Aβ25–35 segment, which recapitulates key aspects of S100B activity, as a starting guide for the analysis. We used Haddock to establish a preferred binding mode, which was studied with the full length Aβ using long (1 μs) molecular dynamics (MD) simulations to investigate the structural dynamics and obtain representative interaction complexes. From the analysis, Aβ-Lys28 emerged as a key candidate for stabilizing interactions with the S100B binding cleft, in particular involving a triad composed of Met79, Thr82 and Glu86. Binding constant calculations concluded that coulombic interactions, presumably implicating the Lys28(Aβ)/Glu86(S100B) pair, are very relevant for the holdase-type chaperone activity. To confirm this experimentally, we examined the inhibitory effect of S100B over Aβ aggregation at high ionic strength. In agreement with the computational predictions, we observed that electrostatic perturbation of the Aβ-S100B interaction decreases anti-aggregation activity. Altogether, these findings unveil features relevant in the definition of selectivity of the S100B chaperone, with implications in Alzheimer’s disease.
Molecular Mechanisms of Canine Osteosarcoma Metastasis
Authors: Wilk Sylwia S. , Zabielska-Koczywąs Katarzyna A.
Published: March 2021

Osteosarcoma (OSA) represents the most common bone tumor in dogs. The malignancy is highly aggressive, and most of the dogs die due to metastasis, especially to the lungs. The metastatic process is complex and consists of several main steps. Assessment of the molecular mechanisms of metastasis requires in vitro and especially in vivo studies for a full evaluation of the process. The molecular and biological resemblance of canine OSA to its human counterpart enables the utilization of dogs as a spontaneous model of this disease in humans. The aim of the present review article is to summarize the knowledge of genes and proteins, including p63, signal transducer and activator of transcription 3 (STAT3), Snail2, ezrin, phosphorylated ezrin-radixin-moesin (p-ERM), hepatocyte growth factor-scatter factor (HGF-SF), epidermal growth factor receptor (EGFR), miR-9, and miR-34a, that are proven, by in vitro and/or in vivo studies, to be potentially involved in the metastatic cascade of canine OSA. The determination of molecular targets of metastatic disease may enhance the development of new therapeutic strategies.
The Antithrombotic Agent Pterostilbene Interferes with Integrin αIIbβ3-Mediated Inside-Out and Outside-In Signals in Human Platelets
Authors: Huang Wei-Chieh , Lin Kao-Chang , Hsia Chih-Wei , Hsia Chih-Hsuan , Chen Ting-Yu , Bhavan Periyakali Saravana , Sheu Joen-Rong , Hou Shaw-Min
Published: March 2021

Platelets play a crucial role in the physiology of primary hemostasis and pathological processes such as arterial thrombosis; thus, developing a therapeutic target that prevents platelet activation can reduce arterial thrombosis. Pterostilbene (PTE) has remarkable pharmacological activities, including anticancer and neuroprotection. Few studies have reported the effects of pterostilbene on platelet activation. Thus, we examined the inhibitory mechanisms of pterostilbene in human platelets and its role in vascular thrombosis prevention in mice. At low concentrations (2–8 μM), pterostilbene strongly inhibited collagen-induced platelet aggregation. Furthermore, pterostilbene markedly diminished Lyn, Fyn, and Syk phosphorylation and hydroxyl radical formation stimulated by collagen. Moreover, PTE directly hindered integrin αIIbβ3 activation through interfering with PAC-1 binding stimulated by collagen. In addition, pterostilbene affected integrin αIIbβ3-mediated outside-in signaling, such as integrin β3, Src, and FAK phosphorylation, and reduced the number of adherent platelets and the single platelet spreading area on immobilized fibrinogen as well as thrombin-stimulated fibrin clot retraction. Furthermore, pterostilbene substantially prolonged the occlusion time of thrombotic platelet plug formation in mice. This study demonstrated that pterostilbene exhibits a strong activity against platelet activation through the inhibition of integrin αIIbβ3-mediated inside-out and outside-in signaling, suggesting that pterostilbene can serve as a therapeutic agent for thromboembolic disorders.
A Social Media Mining and Ensemble Learning Model: Application to Luxury and Fast Fashion Brands
Authors: Chen Yulin
Published: March 2021

This research proposes a framework for the fashion brand community to explore public participation behaviors triggered by brand information and to understand the importance of key image cues and brand positioning. In addition, it reviews different participation responses (likes, comments, and shares) to build systematic image and theme modules that detail planning requirements for community information. The sample includes luxury fashion brands (Chanel, Hermès, and Louis Vuitton) and fast fashion brands (Adidas, Nike, and Zara). Using a web crawler, a total of 21,670 posts made from 2011 to 2019 are obtained. A fashion brand image model is constructed to determine key image cues in posts by each brand. Drawing on the findings of the ensemble analysis, this research divides cues used by the six major fashion brands into two modules, image cue module and image and theme cue module, to understand participation responses in the form of likes, comments, and shares. The results of the systematic image and theme module serve as a critical reference for admins exploring the characteristics of public participation for each brand and the main factors motivating public participation.
Relative Efficiency of Pitfall vs. Bait Trapping for Capturing Taxonomic and Functional Diversities of Ant Assemblages in Temperate Heathlands
Authors: Hacala Axel , Gouraud Clément , Dekoninck Wouter , Pétillon Julien
Published: March 2021

Whereas bait and pitfall trappings are two of the most commonly used techniques for sampling ant assemblages, they have not been properly compared in temperate open habitats. In this study, taking advantage of a large-scale project of heathland restoration (three sites along the French Atlantic Coast forming a north-south gradient), we evaluated the relative efficiency of these two methods for assessing both taxonomic and functional diversities of ants. Ants were collected and identified to species level, and six traits related to morphology, behavior (diet, dispersal and maximum foraging distance), and social life (colony size and dominance type) were attributed to all 23 species. Both observed and estimated species richness were significantly higher in pitfalls compared to spatially pair-matched bait traps. Functional richness followed the same pattern, with consistent results for both community weighted mean (CWM) and Rao’s quadratic entropy. Taxonomic and functional diversities from pitfall assemblages increased from north to south locations, following a pattern frequently reported at larger spatial scales. Bait trapping can hardly be considered a complementary method to pitfall trapping for sampling ants in open temperate habitats, as it appears basically redundant with the latter sampling method, at least in coastal heathlands of the East-Atlantic coast.
Differences in the Susceptibility to Commercial Insecticides among Populations of the Lesser Mealworm Alphitobius diaperinus Collected from Poultry Houses in France
Authors: Renault David , Colinet Hervé
Published: March 2021

The control of insect pests often relies on the recurrent application of insecticides. This is the case for the lesser mealworm, Alphitobius diaperinus, an invasive beetle infesting poultry farms. There is evidence that A. diaperinus can develop resistance to several insecticides. Evaluation of such resistance has never been conducted in France, despite the beetle’s presence since the 1970s. We assess insecticide susceptibility in 10 populations from French poultry farms and compare patterns with two susceptible populations. Adults are subjected to short-term exposures (4 h) to four commercial insecticides and their recovery is assessed. Temporal survival also is scored during constant exposures for seven days. Clear-cut differences among the farm populations are found. Except for three populations that have patterns similar to those of the two susceptible populations, all the other farm populations have a much greater capacity to recover and survive insecticide exposures, especially to pyrethroid-based formulations. Three populations in particular even exhibit clear signs of resistance to pyrethroids, with median lethal times more than 10-fold superior to values of the susceptible population. No insect in any population recovers from the pirimiphos-methyl exposure, and all beetles are apparently dead after 15 h. Our results demonstrate the existence of resistant populations to pyrethroids in Brittany France.
Eccentric Force-Velocity Characteristics during a Novel Squat Protocol in Trained Rugby Union Athletes—Pilot Study
Authors: McNeill Conor , Beaven C. Martyn , McMaster Daniel T. , Gill Nicholas
Published: March 2021

Eccentric strength characteristics have been shown to be important factors in physical performance. Many eccentric tests have been performed in isolation or with supramaximal loading. The purpose of this study was to investigate within- and between- session reliability of an incremental eccentric back squat protocol. Force plates and a linear position transducer captured force-time-displacement data across six loading conditions, separated by at least seven days. The reliability of eccentric specific measurements was assessed using coefficient of variation (CV), change in mean, and intraclass correlation coefficient (ICC). Eccentric peak force demonstrated good ICC (≥0.82) and TE (≤7.3%) for each load. Variables based on mean data were generally less reliable (e.g., mean rate of force development, mean force, mean velocity). This novel protocol meets acceptable levels of reliability for different eccentric-specific measurements although the extent to which these variables affect dynamic performance requires further research.