bluuebunny/crossref_metadata_2025 huggingface dataset:
A Predicting Model for the Effective Thermal Conductivity of Anisotropic Open-Cell Foam
Authors: Zhang Chao , Kong Xiangzhuang , Wang Xian , Du Yanxia , Xiao Guangming
Published: August 2022

The structural anisotropy of open-cell foam leads to the anisotropy of effective thermal conductivity (ETC). To quantitatively analyze the effect of structural anisotropy on the anisotropy of ETC, a new predicting model for the ETC of anisotropic open-cell foam was proposed based on an anisotropy tetrakaidecahedron cell (ATC). Feret diameters in three orthogonal directions obtained by morphological analysis of real foam structures were used to characterize the anisotropy of ATC. To validate our proposed anisotropic model, the ETCs of real foam structures in three orthogonal directions predicted by it were compared with the numerical results, for which the structures of numerical models are reconstructed by X-ray computed tomography (X-CT). Using the present anisotropic model, the influences of the thermal conductivity ratio (TCR) and porosity of the foams on the anisotropic ratios of ETCs are also investigated. Results show that there is good consistency between the ETCs obtained by the anisotropic model and the numerical method. The maximum relative errors between them are 2.84% and 13.57% when TCRs are 10 and 100, respectively. The present anisotropic model can not only predict the ETCs in different orthogonal directions but also quantitatively predict the anisotropy of ETC. The anisotropies of the ETCs decrease with porosity because the proportion of the foam skeleton decreases. However, the anisotropies of ETCs increase with TCR, and there exist asymptotic values in anisotropic ratios of ETCs as TCR approaches infinity and they are equal to the relative Feret diameters in different orthogonal directions.
Towards Data-Driven Models in the Prediction of Ship Performance (Speed—Power) in Actual Seas: A Comparative Study between Modern Approaches
Authors: Alexiou Kiriakos , Pariotis Efthimios G. , Leligou Helen C. , Zannis Theodoros C.
Published: August 2022

In the extremely competitive environment of shipping, minimizing shipping cost is the key factor for the survival and growth of shipping companies. However, stricter rules and regulations that aim at the reduction of greenhouse gas emissions published by the International Maritime Organization, force shipping companies to increase the operational efficiency of their fleet. The prediction of a ship speed in actual seas with a given power by its engine is the most important performance indicator and thus makes it the “holy grail” in pursuing better efficiency. Traditionally, tank model tests and semi-empirical formulas were the preferred solution for the aforementioned prediction and are still widely applied. However, currently, with the increased computational power that is widely available, novel and more sophisticated methods taking into consideration computational fluid dynamics (CFD) and machine learning (ML) algorithms are emerging. In this paper, we briefly present the different approaches in the prediction of a ship’s speed but focus on ML methods comparing a representative number of the latest data-driven models used in papers, to provide guidelines, discover trends and identify the challenges to be faced by researchers. From this comparison, we can distinguish that artificial neural networks (ANN), being used in 73.3% of the reviewed papers, dominate as the algorithm of choice. Researchers mostly rely on physical laws governing the phenomena in the crucial part of data preprocessing tasks. Lastly, most researchers rely on data acquisition systems installed at ships in order to achieve usable results.
There Is Plenty of Room inside a Bluff Body: A Hybrid Piezoelectric and Electromagnetic Wind Energy Harvester
Authors: Al-Riyami Mahmood , Bahadur Issam , Ouakad Hassen
Published: August 2022

In this paper, a piezoelectric and electromagnetic hybrid wind energy harvester is proposed. The general design of the harvester comprises multiple cantilever piezoelectric energy harvesters (PEHs) and electromagnetic energy harvesters (EEHs) embedded inside the bluff body that is attached to the free end of PEHs. This research work investigates utilizing the room inside the bluff body to enclose harvesters to have a more compact and efficient harvesting system. A comprehensive coupled dynamic model of the harvester (HEH) is developed using Lagrange’s formulation. The electromechanical and electromagnetic coupling coefficient equations are derived. The coupled equations of motion are solved analytically and numerically with an exact agreement. A parametric analysis is conducted to study the effect of the design parameters on the overall performance of the harvester in terms of output power and bandwidth. The proposed design evidently presents itself as a promising concept in utilizing the room inside a bluff body.
Design, Greenhouse Emissions, and Environmental Payback of a Photovoltaic Solar Energy System
Authors: Schultz Herwin Saito , Carvalho Monica
Published: August 2022

This study aims to design a 16.4 MW photovoltaic solar system located in the Brazilian Northeast and quantify the associated greenhouse gas emissions and environmental payback. The energy system was designed to minimize the Levelized Cost of Energy. The greenhouse gas emissions were quantified with the Life Cycle Assessment methodology, expressing the environmental impact in terms of generated energy (kg CO2-eq/kWh) and following ISO 14040 and 14044. The environmental payback considered the Brazilian electricity mix and degradation of the panels. The results indicated a system capable of producing 521,443 MWh in 25 years, with an emission factor of 0.044 kg CO2-eq/kWh and environmental payback of five years and eight months. The emission factor is at least ten times lower than thermoelectric natural gas power plants. The solar panels were the main contributors to the greenhouse gas emissions, representing 90.59% of overall emissions. It is concluded that photovoltaic energy systems are crucial in the search for emissions mitigation, even in a country that presents a predominantly renewable electricity matrix, with demonstrated environmental benefits.
Higher Cognition: A Mechanical Perspective
Authors: Friedman Robert
Published: August 2022

Cognition is the acquisition of knowledge by the mechanical process of information flow in a system. In cognition, input is received by the sensory modalities and the output may occur as a motor or other response. The sensory information is internally transformed to a set of representations, which is the basis for downstream cognitive processing. This is in contrast to the traditional definition based on mental processes, a phenomenon of the mind that originates in past ideas of philosophy.
De Novo Polycomb Recruitment and Repressive Domain Formation
Authors: Hernández-Romero Itzel Alejandra , Valdes Victor Julian
Published: August 2022

Every cell of an organism shares the same genome; even so, each cellular lineage owns a different transcriptome and proteome. The Polycomb group proteins (PcG) are essential regulators of gene repression patterning during development and homeostasis. However, it is unknown how the repressive complexes, PRC1 and PRC2, identify their targets and elicit new Polycomb domains during cell differentiation. Classical recruitment models consider the pre-existence of repressive histone marks; still, de novo target binding overcomes the absence of both H3K27me3 and H2AK119ub. The CpG islands (CGIs), non-core proteins, and RNA molecules are involved in Polycomb recruitment. Nonetheless, it is unclear how de novo targets are identified depending on the physiological context and developmental stage and which are the leading players stabilizing Polycomb complexes at domain nucleation sites. Here, we examine the features of de novo sites and the accessory elements bridging its recruitment and discuss the first steps of Polycomb domain formation and transcriptional regulation, comprehended by the experimental reconstruction of the repressive domains through time-resolved genomic analyses in mammals.
Characterization of Saccharomyces Strains Isolated from “Kéknyelű” Grape Must and Their Potential for Wine Production
Authors: Gerőcs Annamária , Nagy Tibor , Nemes-Barnás Katalin , Májer János , Szőke Barna Árpád , Kővágó Róbert , Magalhães Frederico , Gibson Brian , Szekeres András , Juhász Ákos , Posta Katalin , Olasz Ferenc
Published: August 2022

Novel wine yeast strains have the potential to satisfy customer demand for new sensorial experiences and to ensure that wine producers have strains that can produce wine as efficiently as possible. In this respect, hybrid yeast strains have recently been the subject of intense research, as they are able to combine the favourable characteristics of both parental strains. In this study, two Saccharomyces “Kéknyelű” grape juice isolates were identified by species-specific PCR and PCR-RFLP methods and investigated with respect to their wine fermentation potential. Physiological characterization of the isolated strains was performed and included assessment of ethanol, sulphur dioxide, temperature and glucose (osmotic stress) tolerance, killer-toxin production, glucose fermentation ability at 16 °C and 24 °C, and laboratory-scale fermentation using sterile “Kéknyelű” must. Volatile components of the final product were studied by gas chromatography (GC) and mass spectrometry (MS). One isolate was identified as a S. cerevisiae × S. kudriavzevii hybrid and the other was S. cerevisiae. Both strains were characterized by high ethanol, sulphur dioxide and glucose tolerance, and the S. cerevisiae strain exhibited the killer phenotype. The hybrid isolate showed good glucose fermentation ability and achieved the lowest residual sugar content in wine. The ester production of the hybrid strain was high compared to the control S. cerevisiae starter strain, and this contributed to the fruity aroma of the wine. Both strains have good oenological characteristics, but only the hybrid yeast has the potential for use in wine fermentation.
Development of Saturated Fat Replacers: Conventional and Nano-Emulsions Stabilised by Lecithin and Hydroxylpropyl Methylcellulose
Authors: Kampa Jansuda , Frazier Richard , Rodriguez-Garcia Julia
Published: August 2022

The combination of two emulsifiers, lecithin and hydroxypropyl methylcellulose (HPMC), into emulsions is an interesting strategy to design fat replacers in food matrices. The objective of this study was to investigate the effect of HPMC type and concentration on the formation, stability, and microstructure of conventional emulsions and nanoemulsions. Two different types of HPMC with low and high content of methyl and hydroxypropyl groups (HPMC-L and HPMC-H) were evaluated. The results showed that the molecular structure and concentration of HPMC play a major role in the viscoelastic behaviour, the gelation temperature, and the strength of gel formed. The firmness and work of shear of HPMC solutions increased significantly (p < 0.05) with increasing concentration. HPMC-L illustrated a more stable gel structure than the HPMC-H solution. Nanoemulsions showed lower moduli values, firmness, and work of shear than conventional emulsions due to the influence of high-pressure homogenization. A combination of lecithin and HPMC improved the physical and lipid oxidative stability of the emulsions, presenting a lower creaming index and thiobarbituric acid reactive substances (TBARS). In conclusion, HPMC-L at 2% w/w could be a suitable type and concentration combined with lecithin to formulate a saturated fat replacer that could mimic butter technological performance during food manufacturing operations.
Evaluating the Impacts of Climate Factors and Flavonoids Content on Chinese Prickly Ash Peel Color Based on HPLC-MS and Structural Equation Model
Authors: Zheng Tao , Zhang Ding-Ling , Sun Bing-Yin , Liu Shu-Ming
Published: August 2022

Climate affects Chinese prickly ash peel color directly through temperature and illumination and indirectly influences it through its effect on flavonoid compounds. In this study, a comprehensive evaluation strategy based on high performance liquid chromatography-mass spectrometry (HPLC-MS) technology and a structural equation model was applied to evaluate the effects of climate factors and flavonoids on Chinese prickly ash peel color. There were obvious geographical variations of peel color and flavonoid compounds with an obvious east–west distribution trend which were divided into high-altitude type and low-altitude type. Through path analysis, the wind speed, temperature and annual sunshine duration were found to be the key environmental factors affecting the flavonoids content and peel color, and their direct effects were higher than their indirect effect. Based on HPLC-MS technology and a structural equation model, correlation models of climatic factors and flavonoids with peel color were established, and the factors that had greater weight on pericarp color were obtained. Our results provide experimental evidence that climate factors affect the peel color by affecting flavonoid biosynthesis and accumulation, reveal the geographical variation of peel color and flavonoid component contents in Chinese prickly ash peel, establish a quantization color method for rapid evaluation of peel quality, expand on the influence of climatic factors on flavonoids content and peel coloration and promote agricultural practice in areas with similar climatic conditions.
The Effect of Microwave Radiation on the Green Color Loss of Green Tea Powder
Authors: Wang Huijuan , Zhu Yan , Xie Dongchao , Zhang Haihua , Zhang Yahui , Jin Peng , Du Qizhen
Published: August 2022

Microwave radiation is one of the main heating methods for food processing, especially affecting the color quality of colorful foods. This work presents the effect of microwave radiation on the green color loss of green tea powder (GTP) by the color description (L*, a*, b*, and Ha of green tea powder, L*:whiteness/darkness, a*: redness/greenness, and b*: yellowness/blueness; Ha derived from Hunter a and b could visually describe the color space) of the Hunter color system. First, the L*, a*, and b* were determined from the GTP samples treated with various microwave powers with the change of time to investigate the kinetic of color loss. Then, the L*, a*, and b*and temperature of GTP samples with serious thickness treated with constant microwave power (700 W) for a different time were determined to study the effect of sample thickness on the color loss. Finally, the chemicals that contributed to color change in the GTP samples treated with mild, moderate, and severe radiation were analyzed. The results showed that L*, |a*| (|a*|was the absolute value of a*), b*, and Ha decreased with the power increase in microwave radiation, and their changes conformed to the first-order kinetics. The activation energies (Ea) of different thickness GTP for change of L*, a*, b*, and Ha values could be predicted with the fitting models, and Ea for 20 mm-thick GTP were approximately 1/5, 1/8, 1/8, and 1/13 of those for 4 mm-thick GTP. The color loss was mainly caused by the Mg2+ loss of chlorophylls and the formation of derivates under mild radiation, the degradation of chlorophylls and the formation of theaflavin from catechins under moderate radiation, and the degradation of chlorophylls and their derivates accompanied by Maillard reaction between reducing sugar and amino acids under severe radiation. The results indicate that sample thickness and radiation time are two key parameters to keeping the color of GTP in food processing and microwave pasteurization.
Chlorophyllin-Based 405 nm Light Photodynamic Improved Fresh-Cut Pakchoi Quality at Postharvest and Inhibited the Formation of Biofilm
Authors: Zhang Yuchen , Ding Zhaoyang , Shao Changbo , Xie Jing
Published: August 2022

The aim of this study was to evaluate the effect of chlorophyllin-based photodynamic inactivation (Chl-PDI) on biofilm formation and fresh-cut pakchoi quality during storage. Firstly, Chl-based PDI reduced the amount of biofilm in an in vivo experiment and inactivated the food spoilage bacteria. Antibacterial mechanism analysis indicated that the bacterial extracellular polysaccharides and extracellular proteins were vulnerable targets for attacks by the Chl-based PDI. Then, the food spoilage microorganisms (Pseudomonas reinekei and Pseudomonas palleroniana) were inoculated onto the surface of fresh-cut pakchoi. We used chlorophyllin (1 × 10−5 mol/L) and 405 nm light (22.27 J/cm2 per day) to investigate the effect of Chl-based PDI treatment on fresh-cut pakchoi quality during storage. The results showed that Chl-based PDI increased the visual quality and the content of chlorophyll, VC, total soluble solids, and SOD activity and decreased the occurrence of leaf yellowing and POD activity. These suggest that Chl-based PDI can be used for the preservation of fresh-cut pakchoi and has the potential to inhibit biofilm formation of food spoilage bacteria. It is of great significance for the effective processing and traditional vegetable preservation.
Towards Better Pharmaceutical Provision in Europe—Who Decides the Future?
Authors: Horgan Denis , Spanic Tanja , Apostolidis Kathi , Curigliano Giuseppe , Chorostowska-Wynimko Joanna , Dauben Hans-Peter , Lal Jonathan A. , Dziadziuszko Rafal , Mayer-Nicolai Christine , Kozaric Marta , Jönsson Bengt , Gutierrez-Ibarluzea Iñaki , Fandel Marie-Helene , Lopert Ruth
Published: August 2022

Significant progress has been achieved in human health in the European Union in recent years. New medicines, vaccines, and treatments have been developed to tackle some of the leading causes of disease and life-threatening illnesses. It is clear that investment in research and development (R&D) for innovative medicines and treatments is essential for making progress in preventing and treating diseases. Ahead of the legislative process, which should begin by the end of 2022, discussions focus on how Europe can best promote the huge potential benefits of new science and technology within the regulatory framework. The challenges in European healthcare were spelled out by the panellists at the roundtable organised by European Alliance for Personalised Medicine (EAPM). Outcomes from panellists’ discussions have been summarized and re-arranged in this paper under five headings: innovation, unmet medical need, access, security of supply, adapting to progress, and efficiency. Some of the conclusions that emerged from the panel are a call for a better overall holistic vision of the future of pharmaceuticals and health in Europe and a collaborative effort among all stakeholders, seeing the delivery of medicines as part of a broader picture of healthcare.
Digital Care Programs for Chronic Hip Pain: A Prospective Longitudinal Cohort Study
Authors: Janela Dora , Costa Fabíola , Areias Anabela C. , Molinos Maria , Moulder Robert G. , Lains Jorge , Bento Virgílio , Scheer Justin K. , Yanamadala Vijay , Cohen Steven P. , Correia Fernando Dias
Published: August 2022

Chronic hip pain is a cause of disability worldwide. Digital interventions (DI) may promote access while providing proper management. This single-arm interventional study assesses the clinical outcomes and engagement of a completely remote multimodal DI in patients with chronic hip pain. This home-based DI consisted of exercise (with real-time biofeedback), education, and cognitive-behavioral therapy. Outcomes were calculated between baseline and program end, using latent growth curve analysis. Primary outcome was the Hip Disability and Osteoarthritis Outcome Score (HOOS). Secondary outcomes were pain, intent to undergo surgery, mental health, productivity, patient engagement (exercise sessions frequency), and satisfaction. Treatment response was assessed using a 30% pain change cut-off. A completion rate of 74.2% (396/534), alongside high patient engagement (2.9 exercise sessions/week, SD 1.1) and satisfaction (8.7/10, SD 1.6) were observed. Significant improvements were observed across all HOOS sub-scales (14.7–26.8%, p < 0.05), with 66.8% treatment responders considering pain. Marked improvements were observed in surgery intent (70.1%), mental health (54%), and productivity impairment (60.5%) (all p < 0.001). The high engagement and satisfaction reported after this DI, alongside the clinical outcome improvement, support the potential of remote care in the management of chronic hip conditions.
Promoting University Students’ Mental Health through an Online Multicomponent Intervention during the COVID-19 Pandemic
Authors: Theurel Anne , Witt Arnaud , Shankland Rebecca
Published: August 2022

The mental health of university students is a serious public health issue. The alarming trend of high levels of untreated psychological distress observed during the COVID-19 pandemic highlights the need for prevention programs. Digital tools are a promising means of delivering such programs. Web-based programs are acceptable and effective at improving mental health problems and general mental well-being. However, the usefulness of such digital prevention approaches to address the multiple issues raised by the COVID-19 pandemic needs to be tested. The current study assessed the effectiveness of an 8-week online intervention, integrating a variety of evidence-based strategies for improving French university students’ mental health. Students were assigned to: (1) the online self-help program ETUCARE (n = 53), or (2) the control condition (n = 50). All the participants completed pre- and post-intervention questionnaires that assessed mental health problems and psychological well-being. The findings revealed that, compared to the control group, participation in the online program was associated with higher levels of psychological well-being post-test and fewer clinical symptoms of psychological distress, anxiety, and alcohol consumption. These preliminary findings suggest that the ETUCARE program is a promising multicomponent intervention to buffer the mental health consequences of the COVID-19 pandemic in French university students.
Fatty Liver Change in Korean Adults in a Systematic Social Distancing System Amid the COVID-19 Pandemic: A Multicenter Analysis
Authors: Haam Ji-Hee , Hur Yang-Im , Kim Young-Sang , Kim Kyoung-Kon , Kang Jee-Hyun , Ko Hae-Jin , Cho Yoon Jeong , Choi Hye-In , Lee Kyu Rae , Park Jung Ha , Cho Soo Hyun , Kim Jong-Koo , Lee Taesic , Seo Myung-Jae , Yoon Yeong Sook , Seo Yoobin , Nam Ga Eun , Kim Sun Hyun
Published: August 2022

In response to the COVID-19 pandemic, the Korean government implemented policies including the systematic social distancing (SSD) system which started on 28 June 2020. The present study investigated the development and aggravation of fatty liver measured using ultrasonography during the transition period (from pre-SSD to SSD) compared to the fatty liver changes during the pre-SSD period. Changes in fatty liver and liver enzymes were assessed in different groups stratified by alcohol consumption. Our retrospective cohort analysis included 5668 participants who underwent health checkups at 13 university hospitals during the SSD period and two or more checkups before the SSD period. Fatty liver developed and aggravated more in the transition period (13.6% development and 12.0% aggravation) than in the pre-SSD period (10.8% development and 10.1% aggravation) in the alcohol consumption group. This finding was more prominent in women than in men. Abnormal alanine transaminase levels were more often developed in the transition period than in the pre-SSD period, especially in men (11.1% vs. 8.6% in each period). In conclusion, the SSD system may contribute to fatty liver changes in individuals who regularly consume alcohol. Further research of the post-pandemic period is needed to assess long-term changes in fatty liver disease.
Family and Educational Strategies for Cyberbullying Prevention: A Systematic Review
Authors: Tozzo Pamela , Cuman Oriana , Moratto Eleonora , Caenazzo Luciana
Published: August 2022

Cyberbullying can be described as a form of bullying carried out by an individual or a group through digital media with the intention to harm others. It has been recognized as a public health issue recently; however, of the vast literature published in recent years on the phenomenon, only a small part concerns strategies adopted to prevent and combat cyberbullying, and the effectiveness of these strategies appears to be scarce. We conducted a systematic review of the literature published in the last five years about different interventions studied to prevent and contrast cyberbullying. Our results show how most of the strategies currently developed focus on the educational aspect, involving schools and families. Other authors describe technology-based practices to set programs to reduce and prevent cyberbullying through the usage of digital instruments, the same used by minors themselves. Finally, remaining tactics use a more comprehensive approach, mixing tools already in use in the aforementioned strategies. Cyberbullying requires wide-ranging methods to combat it, involving the contribution of mental health professionals, educators, and digital experts cooperating synergically. Prevention and contrast instruments should be defined, implemented, tested, and combined in order to deal with cyberbullying.
Sex Differences between Urinary Phthalate Metabolites and Metabolic Syndrome in Adults: A Cross-Sectional Taiwan Biobank Study
Authors: Shih Ya-Ling , Hsieh Chia-Jung , Lee Tso-Ying , Liao Pei-Hung , Wu Hao-Ting , Liu Chieh-Yu
Published: August 2022

Background: Phthalates are widely used in consumer products, food packaging, and personal care products, so exposure is widespread. Several studies have investigated the association of phthalate exposure with obesity, insulin resistance, and hypertension. However, little is known about the associations of phthalate exposure with sex, age, and menopausal status in metabolic syndrome (MetS). The purpose of this study was to investigate the association between 11 urinary phthalate metabolite concentrations and metabolic syndrome in adults. Methods: We conducted a cross-sectional analysis of 1337 adults aged 30–70 years from the Taiwan Biobank 2016–2020. Prevalence odds ratios (POR) and 95% confidence intervals (CIs) were calculated using logistic regression and stratified by sex, age, and menopausal status. Results: Participants with MetS comprised 16.38%. Higher concentrations of MEP metabolites were associated with more than two- to three-fold increased odds of MetS in males and males ≥ 50 years (adj. POR Q3 vs. Q1 = 2.13, 95% CI: 1.01, 4.50; p = 0.047 and adj. POR Q2 vs. Q1 = 3.11, 95% CI: 0.13, 8.63; p = 0.029). When assessed by menopausal status, postmenopausal females with higher ∑DEHP concentrations had more than nine-fold higher odds of MetS compared with postmenopausal females with the lowest ∑DEHP concentrations (adj. POR Q3 vs. Q1 = 9.58, 95% CI: 1.18, 77.75; p = 0.034). Conclusions: The findings suggest differential associations between certain phthalate metabolites and MetS by sex, age, and menopausal status.
Identification and Assessment of the Driving Forces behind Changes in the Foothill Landscape: Case Studies of the Mysłakowice and Jelenia Góra Communities in Poland
Authors: Krajewski Piotr , Lebiedzińska Monika , Kołodyńska Iga
Published: August 2022

The main objective of this study was to determine the driving forces behind landscape change and the perceptions of change by the residents of selected research areas. The communities used for the study were Mysłakowice and Jelenia Góra, located in the Lower Silesia region in Poland. Mysłakowice is a rural community, and Jelenia Góra is an urban community. The landscape of both municipalities is dominated by forest-covered mountains surrounding dispersed built-up and agricultural areas. The time range of the analysis was 2005–2020, covering the period after Poland’s accession to the European Union, and was divided into the following three time periods: 2005–2010, 2010–2015, and 2015–2020. The research methodology consisted of the following three stages: (1) the identification of landscape changes on the basis of land cover data and the calculation of the landscape change index (LCI), (2) the characterization and classification of the identified landscape changes, and (3) the identification of the driving forces of landscape changes through surveys with the residents of both municipalities. The results obtained based on the surveys were often consistent with the results from the GIS analysis. The respondents were able to identify the most important changes and proposed the driving forces affecting them. According to the residents of Mysłakowice and Jelenia Góra, the changes in the landscape between 2005 and 2020 were primarily the result of political and socio-economic driving forces, accompanied by forces from other groups. However, each time period was distinctive. The analysis showed which types of changes in the landscape were viewed positively and negatively by the people during the analyzed periods of time, and what the influence of the different driving forces was on the formation of changes in the landscape.
Dynamic Asymmetric Effect of Currency Risk Pricing of Exchange Rate on Equity Markets: A Regime-Switching Based C-Vine Copulas Method
Authors: Mudiangombe Benjamin Mudiangombe , Muteba Mwamba John Weirstrass
Published: August 2022

This paper investigates whether currency risk is priced differently in the different sectors (industrial, financial, and basic materials) of equity markets in a sample of developed United States of America (USA) and developing economies (Brazil, India, Poland, and South Africa). The paper makes use of the following techniques: (i) Univariate Autoregressive Fractionally Integrated Moving Average and Exponential General Autoregressive Conditional Heteroskedastic (ARFIMA-EGARCH), (ii) the Markov Switching method (MS), and (iii) the Canonical Vine Copulas (C-Vine) techniques. Using a sample of daily data made of the foreign exchange rate against the domestic currency and equity market sectors; our findings show that there is an asymmetry effect between equity markets and the foreign exchange rate: there is a heterogeneous, strong, and positive dependence between the two. Higher equity prices are associated with depreciation of local currencies, according to US dollar (USD) exchange rates. In addition, we find that the selected emerging economies are pricing a positive and considerable currency risk. The pricing of currency risk has a varied effect in both regimes representing the states of the economy. In fact, when currency risk pricing has a beneficial impact on certain sectors of the economy, investors predict better returns.
Make It Simple: Effective Road Selection for Small-Scale Map Design Using Decision-Tree-Based Models
Authors: Karsznia Izabela , Wereszczyńska Karolina , Weibel Robert
Published: August 2022

The complexity of a road network must be reduced after a scale change, so that the legibility of the map can be maintained. However, deciding whether to show a particular road section on the map is a very complex process. This process, called selection, constitutes the first step in a sequence of further generalization operations and it is a prerequisite to effective road network generalization. So far, not many comprehensive solutions have been developed for effective road selection specifically at small scales as the studies have mainly dealt with large-scale maps. The paper presents an experiment using machine learning (ML), specifically decision-tree-based (DT) models, to optimize the selection of the roads from 1:250,000 to 1:500,000 and 1:1,000,000 scales. The scope of this research covers designing and verifying road selection models on the example of three selected districts in Poland. The aim is to consider the problem of road generalization holistically, including numerous semantic, geometric, topological, and statistical road characteristics. The research resulted in a list of measurable road attributes that comprehensively describe the rank of a particular road section. The outcome also includes attribute weights, attribute correlation calculated for roads, and machine learning models designed for automatic road network selection. The performance of the machine learning models is very high and ranges from 80.94% to 91.23% for the 1:500,000 target scale and 98.21% to 99.86% for the 1:1,000,000 scale.
Histology of Cerebral Clots in Cryptogenic Stroke Varies According to the Presence of a Patent Foramen Ovale
Authors: Härtl Johanna , Berndt Maria , Poppert Holger , Liesche-Starnecker Friederike , Steiger Katja , Wunderlich Silke , Boeckh-Behrens Tobias , Ikenberg Benno
Published: August 2022

Although a pathophysiological impact remains difficult to prove in individual patient care, a patent foramen ovale (PFO) is currently considered of high relevance for secondary prophylaxis in selected patients with cryptogenic ischemic stroke. By quantification of histological clot composition, we aimed to enhance pathophysiological understanding of PFO attributable ischemic strokes. Retrospectively, we evaluated all cerebral clots retrieved by mechanical thrombectomy for acute ischemic stroke treatment between 2011 and 2021 at our comprehensive stroke care center. Inclusion criteria applied were cryptogenic stroke, age (≤60 years), and PFO status according to transesophageal echocardiography, resulting in a study population of 58 patients. Relative clot composition was calculated using orbit image analysis to define the ratio of main histologic components (fibrin/platelets (F/P), red blood cell count (RBC), leukocytes). Cryptogenic stroke patients with PFO (PFO+, n = 20) displayed a significantly higher percentage of RBC (0.57 ± 0.17; p = 0.002) and lower percentage of F/P (0.38 ± 0.15; p = 0.003) compared to patients without PFO (PFO–, n = 38) (RBC: 0.41 ± 0.21; F/P: 0.52 ± 0.20). In conclusion, histologic clot composition in cryptogenic stroke varies depending on the presence of a PFO. Our findings histologically support the concept that a PFO may be of pathophysiological relevance in cryptogenic ischemic stroke.
Azithromycin and Ceftriaxone Differentially Activate NLRP3 in LPS Primed Cancer Cells
Authors: Tezcan Gulcin , Alsaadi Mohammad , Hamza Shaimaa , Garanina Ekaterina E. , Martynova Ekaterina V. , Ziganshina Gulshat R. , Farukshina Elina R. , Rizvanov Albert A. , Khaiboullina Svetlana F.
Published: August 2022

Background: Cancer patients are prescribed antibiotics, such as macrolides and lactamides, for infection treatment. However, the effect of these antibiotics on NLRP3 activation remains largely unknown. Method: Lung cancer (A549) and prostate cancer (PC3) cell lines were primed with lipopolysaccharide (LPS) to activate NLRP3 transcription. Cells were then treated with azithromycin (Az) or ceftriaxone (Cf). NLRP3 activation was analyzed by qPCR, Western blot, and ELISA. Cell growth and viability were assessed by real-time cell analysis and Annexin V expression. Levels of 41 cytokines were also analyzed using a multiplex assay. Results: LPS-Az activated transcription of NLRP3, Pro-CASP-1, and Pro-IL-1β in A549 cells, while failing to upregulate NLRP3 and Pro-IL-1β in PC3 cells. LPS-Az decreased the secretion of pro-inflammatory cytokines while it induced the pro-angiogenic factors in A549 and PC3 cells. In contrast, LPS-Cf suppressed the expression of NLRP3-associated genes, NLRP3 protein expression, the inflammatory cytokine secretion in A549 and PC3 cells. LPS-Az and LPS-Cf had a limited effect on cell growth and viability. Discussion: Our data suggest that Cf could suppress LPS induced NLRP3, which should be considered when selecting antibiotics for cancer treatment. In contrast, the effect of Az on LPS primed NLRP3 and the inflammatory cytokines production appears to depend on the cancer cell origin. Therefore, these data indicate that considerations are required when selecting Az for the treatment of cancer patients.
Effects of Polymers on the Drug Solubility and Dissolution Enhancement of Poorly Water-Soluble Rivaroxaban
Authors: Choi Min-Jong , Woo Mi Ran , Choi Han-Gon , Jin Sung Giu
Published: August 2022

The purpose of this study was to investigate the efficacy of hydrophilic polymers in a solid dispersion formulation in improving the solubility and dissolution rate of rivaroxaban (RXB), a poorly soluble drug. The developed solid dispersion consisted of two components, a drug and a polymer, and the drug was dispersed as amorphous particles in a polymer matrix using the spray drying method. Polymeric solid dispersions were evaluated using solubility tests, in vitro dissolution tests, powder X-ray diffraction, differential scanning calorimetry, scanning electron microscopy, and particle size distribution analysis. To maximize physical stability against crystallization and improve the solubility and dissolution of RXB, it is important to select the appropriate polymer type and the optimal ratio of the polymer to the drug. The optimized polyvinyl alcohol (PVA)-based (1/0.5, w/w) and gelatin-based (1/5, w/w) solid dispersion formulations showed 6.3 and 3.6 times higher drug solubilities than pure RXB powder, respectively, and the final dissolution rate was improved by approximately 1.5 times. Scanning electron microscopy and particle size distribution analyses confirmed that the gelatin-based solid dispersion was smaller and more spherical than the PVA-based solid dispersion, suggesting that the gelatin-based solid dispersion had a faster initial dissolution rate. Differential scanning calorimetry and powder X-ray diffraction analyses confirmed that RXB had successfully changed from a crystalline form to an amorphous form, contributing to the improvement in its solubility and dissolution rate. This study provides a strategy for selecting suitable polymers for the development of amorphous polymer solid dispersions that can overcome precipitation during dissolution and stabilization of the amorphous state. In addition, the selected polymer solid dispersion improved the drug solubility and dissolution rate of RXB, a poorly soluble drug, and may be used as a promising drug delivery system.
The Catalytic Subunit of Schizosaccharomyces pombe CK2 (Cka1) Negatively Regulates RNA Polymerase II Transcription through Phosphorylation of Positive Cofactor 4 (PC4)
Authors: Rojas Diego A. , Urbina Fabiola , Solari Aldo , Maldonado Edio
Published: August 2022

Positive cofactor 4 (PC4) is a transcriptional coactivator that plays important roles in transcription and DNA replication. In mammals, PC4 is phosphorylated by CK2, and this event downregulates its RNA polymerase II (RNAPII) coactivator function. This work describes the effect of fission yeast PC4 phosphorylation on RNAPII transcription in a cell extract, which closely resembles the cellular context. We found that fission yeast PC4 is strongly phosphorylated by the catalytic subunit of CK2 (Cka1), while the regulatory subunit (Ckb1) downregulates the PC4 phosphorylation. The addition of Cka1 to an in vitro transcription assay can diminish the basal transcription from the Ad-MLP promoter; however, the addition of recombinant fission yeast PC4 or Ckb1 can stimulate the basal transcription in a cell extract. Fission yeast PC4 is phosphorylated in a domain which has consensus phosphorylation sites for CK2, and two serine residues were identified as critical for CK2 phosphorylation. Mutation of one of the serine residues in PC4 does not completely abolish the phosphorylation; however, when the two serine residues are mutated, CK2 is no longer able to phosphorylate PC4. The mutant which is not phosphorylated is able to stimulate transcription even though it is previously phosphorylated by Cka1, while the wild type and the point mutant are inactivated by Cka1 phosphorylation, and they cannot stimulate transcription by RNAPII in cell extracts. Those results demonstrate that CK2 can regulate the coactivator function of fission yeast PC4 and suggests that this event could be important in vivo as well.
Expression of a Hydroxycinnamoyl-CoA Shikimate/Quinate Hydroxycinnamoyl Transferase 4 Gene from Zoysia japonica (ZjHCT4) Causes Excessive Elongation and Lignin Composition Changes in Agrostis stolonifera
Authors: Dong Di , Yang Zhuoxiong , Ma Yuan , Li Shuwen , Wang Mengdi , Li Yinruizhi , Liu Zhuocheng , Jia Chenyan , Han Liebao , Chao Yuehui
Published: August 2022

Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT) is considered to be an essential enzyme for regulating the biosynthesis and composition of lignin. To investigate the properties and function of ZjHCT4, the ZjHCT4 gene was cloned from Zoysia japonica with a completed coding sequence of 1284-bp in length, encoding 428 amino acids. The ZjHCT4 gene promoter has several methyl jasmonate (MeJA) response elements. According to analysis of expression patterns, it was up-regulated by MeJA, GA3 (Gibberellin), and SA (Salicylic acid), and down-regulated by ABA (Abscisic acid). Ectopic ZjHCT4 expression in creeping bentgrass causes excessive plant elongation. In addition, the content of G-lingnin and H-lingnin fell in transgenic plants, whereas the level of S-lingnin increased, resulting in a considerable rise in the S/G unit ratio. Analysis of the expression levels of lignin-related genes revealed that the ectopic expression of ZjHCT4 altered the expression levels of a number of genes involved in the lignin synthesis pathway. Simultaneously, MeJA, SA, GA3, IAA, BR (Brassinosteroid), and other hormones were dramatically enhanced in transgenic plants relative to control plants, whereas ABA concentration was significantly decreased. Expression of ZjHCT4 impacted lignin composition and plant growth via altering the phenylpropionic acid metabolic pathway and hormone response, as revealed by transcriptome analysis. HCTs may influence plant lignin composition and plant development by altering hormone content. These findings contributed to a deeper comprehension of the lignin synthesis pathway and set the stage for further investigation and application of the HCTs gene.
Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes
Authors: Banfalvi Gaspar
Published: August 2022

Eukaryotic chromosomes have not been visualized during the interphase. The fact that chromosomes cannot be seen during the interphase of the cell cycle does not mean that there are no means to make them visible. This work provides visual evidence that reversible permeabilization of the cell membrane followed by the regeneration of cell membranes allows getting a glimpse behind the nuclear curtain. Reversibly permeable eukaryotic cells have been used to synthesize nascent DNA, analyze the 5′-end of RNA primers, view individual replicons and visualize interphase chromosomes. Dextran T-150 in a slightly hypotonic buffer prevented cells from disruption. Upon reversal of permeabilization, the nucleus could be opened at any time during the interphase. A broad spectrum of a flexible chromatin folding pattern was revealed through a series of transient geometric forms of chromosomes. Linear attachment of chromosomes was visualized in several mammalian and lower eukaryotic cells. The linear connection of chromosomes is maintained throughout the cell cycle showing that rather than individual chromosomes, a linear array of chromosomes is the functional giant macromolecule. This study proves that not only the prokaryotic genome but also linearly attached eukaryotic chromosomes form a giant macromolecular unit.
The Mechanism of Energy Coupling in H+/Na+-Pumping Membrane Pyrophosphatase—Possibilities and Probabilities
Authors: Baykov Alexander A. , Anashkin Viktor A. , Malinen Anssi M. , Bogachev Alexander V.
Published: August 2022

Membrane pyrophosphatases (mPPases) found in plant vacuoles and some prokaryotes and protists are ancient cation pumps that couple pyrophosphate hydrolysis with the H+ and/or Na+ transport out of the cytoplasm. Because this function is reversible, mPPases play a role in maintaining the level of cytoplasmic pyrophosphate, a known regulator of numerous metabolic reactions. mPPases arouse interest because they are among the simplest membrane transporters and have no homologs among known ion pumps. Detailed phylogenetic studies have revealed various subtypes of mPPases and suggested their roles in the evolution of the “sodium” and “proton” bioenergetics. This treatise focuses on the mechanistic aspects of the transport reaction, namely, the coupling step, the role of the chemically produced proton, subunit cooperation, and the relationship between the proton and sodium ion transport. The available data identify H+-PPases as the first non-oxidoreductase pump with a “direct-coupling” mechanism, i.e., the transported proton is produced in the coupled chemical reaction. They also support a “billiard” hypothesis, which unifies the H+ and Na+ transport mechanisms in mPPase and, probably, other transporters.
Modeling of the Natural Objects’ Temperature Field Distribution Using a Supercomputer
Authors: Martirosyan Alexander Vitalievich , Ilyushin Yury Valeryevich
Published: August 2022

There are some regions where the unique type of mineral water mining is compounded by the complex structure conditions of the field. Due to the high emergency risks, the automation of this type of mineral water fields’ exploitation process is a necessity. Kislovodskoe mineral water field was chosen as the experimental object, because it has a number of features that make it barely possible to use the conventional methods of mineral water extraction. In the process of extraction, the random vector impacts the mineral water pumping systems. This is mainly due to the complex hydrogeological structure. For the experiment, the laboratory installation and mathematical model were presented by the temperature field changing, due to the similitude of the mathematical apparatus and the hydrodynamic processes behavior,. The main object of this article is the analysis of the reservoir’s temperature field behavior using a hybrid supercomputer, and the differences between the supercomputer and a conventional personal computer modeling quality and implementation costs are also given..
Capacity Assessment in Freight-Passengers Complex Railway Nodes: Trieste Case Study
Authors: Kianinejadoshah Atieh , Ricci Stefano
Published: August 2022

An integrated approach to node and station operation analysis is possible by means of analytical methods, customized to this scope. Alternatively, the simulation models allow more in-depth analyses aiming at the optimization of the use of capacity. The general goals of the research are the comparison of methods for the assessment of railway lines and nodes’ capacity, suitability for specific tasks, and stability of the results under variable scenarios. The comparison is finalised to quantify the relative level of confidence of the concerned literature methods. The work is part of a larger research project with the final goal of identifying the most appropriate approach for the optimization of the network capacity and the setup of specific guidelines. In this framework and perspective, the paper introduces synthetically the methods and applies them systematically to a real complex mixed-traffic network in Trieste, situated in Northeast Italy, including the main passengers and freight stations and a set of lines used for both services.