bluuebunny/crossref_metadata_2025 huggingface dataset:
Alternative Management of Acute Irreversible Pulpitis of an Adult HIV-Positive Patient: A Case Report
Authors: Hussain Muhammad Imran , Bashar A K M , Shikder A H M Zakir Hossain
Published: May 2022

Background: Root canal treatment is the usual management of acute irreversible pulpitis of mature permanent teeth. MTA Pulpotomy can be used to treat acute irreversible pulpitis of an adult HIV-Positive patient alternative to root canal treatment as it is less invasive and one-step endodontic therapy.
Objective: The aim was to evaluate the outcome of MTA Pulpotomy alternative to root canal treatment to manage acute irreversible pulpitis of an adult HIV-positive patient.
Methods: Based on history, clinical and radiological examination the case was diagnosed as acute irreversible pulpitis on the mandibular left 2nd molar tooth in a patient who was HIV-positive and under treatment for 16 years. After anaesthetising and isolation with rubber dam coronal pulp was completely removed and MTA (Angelus, Brazil) was placed over the pulp chamber floor covering the canal orifices, and the rest of the cavity was sealed with glass-ionomer filling over the set MTA. The severity of postoperative pain was assessed by a visual analog scale after 24 hours, 48 hours, 72 hours, and 7 days. The incidence of any clinical symptoms or periapical pathology was determined using clinical and radiographic evaluation after 3-month, 6 months, and 1-year intervals.
Results: Throughout the follow-up period, the patient was asymptomatic and there was no periapical pathology at the radiograph even at the end of one year.
Conclusion: As a less invasive and single sitting short procedure, MTA pulpotomy can be done to treat acute irreversible pulpitis in the HIV-positive patient as an alternative to root canal treatment.
Bangladesh Med Res Counc Bull 2021; 47(2): 230-234
PENGARUH STRATEGI PROBLEM BASED LEARNING DAN MOTIVASI BERPRESTASI TERHADAP KETERAMPILAN BERPIKIR KRITIS
Authors: Supriyanto Supriyanto , Fatirul Achmad Noor , Walujo Djoko Adi
Published: May 2022

ABSTRAK Tujuan penelitian ini yaitu: 1) untuk mengetahui hasil perbedaan kemampuan keterampilan berpikir kritis siswa antara diajar strategi problem based learning dengan inquiry. 2) mengetahui perbedaan antara motivasi berprestasi tinggi dan rendah terhadap keterampilan berpikir kritis siswa. 3) mengetahui perbedaan interaksi antara problem based learning dan motivasi berprestasi terhadap keterampilan berpikir kritis. Populasi penelitian ini 93 siswa kelas XI MIPA SMA Muhammadiyah 2 Surabaya. Sampel diambil secara acak. Sedangkan metode pengumpulan data dengan menggunakan instrument terdiri angket motivasi berprestasi dan tes hasil belajar untuk mengetahui keterampilan berpikir kritis siswa. Analisis data yang digunakan dengan teknik analisis varians dua jalur (ANAVA). Berdasarkan penyajian data dan pembahasan disimpulkan : 1) strategi pembelajaran problem based learning lebih baik dari pada inquiry terhadap keterampilan berfikir kritis siswa. 2) terdapat perbedaan yang signifikan antara motivasi berprestasi tinggi dan motivasi rendah terhadap keterampilan berpikir kritis siswa. 3) strategi pembelajaran dan motivasi berprestasi berpengaruh terhadap keterampilan berpikir kritis siswa. Kata  kunci: problem based learning, inquiry, motivasi berprestasi, berpikir kritis ABSTRACT The aims of this research are: 1) to find out the results of differences in students' critical thinking skills between those taught with problem based learning and inquiry strategies. 2) knowing the difference between high and low achievement motivation on students' critical thinking skills. 3) find out the difference in the interaction between problem based learning and achievement motivation on critical thinking skills. The population in this study were 93 students of class XI MIPA SMA Muhammadiyah 2 Surabaya. Samples were taken randomly. While the data collection method using an instrument consisting of achievement motivation questionnaires and learning outcomes tests to determine students' critical thinking skills. Data analysis used two-way analysis of variance (ANAVA) technique. Based on data presentation and discussion, it can be concluded: 1) problem based learning strategy is better than inquiry on students’ critical thinking skills. 2) there is a significant difference between high achievement motivation and low motivation on strudents’ critical thinking skills. 3) learning strategies and achievement motivation have an effect on students’ critical thinking skills. Keywords: problem based learning, inquiry, achievement motivation, critical thinking
ANALISIS KEBUTUHAN PENGEMBANGAN BAHAN AJAR DIGITAL MATAKULIAH GEOFISIKA BERBASIS PLATFORM LMS MOODLE UNTUK MENUNJANG IMPLEMENTASI KURIKULUM MBKM
Authors: Purwanto Andik , Risdianto Eko
Published: May 2022

ABSTRAK Penelitian ini bertujuan untuk mengetahui kebutuhan mahasiswa Pendidikan Fisika Universitas Bengkulu terhadap bahan ajar digital mata kuliah geofisika untuk menunjang implementasi kurikulum MBKM. Penelitian dilakukan di Program Studi Pendidikan Fisika Universitas Bengkulu pada bulan November 2021. Sampel penelitiannya adalah 47 mahasiswa. Teknik pengumpulan data yang digunakan dalam penelitian analisis kebutuhan ini adalah teknik survey. Instrumen penelitian yang digunakan adalah lembar angket kebutuhan mahasiswa Pendidikan Fisika Universitas Bengkulu terhadap bahan ajar digital matakuliah geofisika. Teknik analisis data yang digunakan adalah analisis statistik deskriptif. Berdasarkan hasil uji validitas dan reliabilitas dinyatakan bahwa angket kebutuhan yang digunakan untuk mengumpulkan data memiliki item-item yang valid dan reliabel. Hasil analisis data menyatakan bahwa mahasiswa sangat setuju dilakukannya pengembangan bahan ajar digital matakuliah geofisika berbasis platform LMS moodle untuk menunjang implementasi kurikulum MBKM. Kata  kunci: bahan ajar digital, geofisika, mbkm ABSTRACT This study aims to determine the needs of Physics Education students at Bengkulu University for digital teaching materials for geophysics courses to support the implementation of the MBKM curriculum. The research was conducted at the Physics Education Study Program at Bengkulu University in November 2021. The research sample was 47 students. The data collection technique used in this needs analysis research is a survey technique. The research instrument used was a questionnaire on the needs of Bengkulu University Physics Education students for digital teaching materials for geophysics courses. The data analysis technique used is descriptive statistical analysis. Based on the results of the validity and reliability test, it is stated that the needs questionnaire used to collect data has valid and reliable items. The results of data analysis state that students strongly agree with the development of digital teaching materials for geophysics courses based on the Moodle LMS platform to support the implementation of the MBKM curriculum. Keywords: digital teaching materials, geophysics, mbkm
PELATIHAN PENDEKATAN GENRE-BASED PADA PEMBELAJARAN SKILL MENULIS BAGI GURU BAHASA INGGRIS SMPN 10 KOTA BENGKULU
Authors: Martina Feny , Afriani Zelvia Liska
Published: May 2022

Kegiatan menulis sering kali masih dianggap problematik dikalangan pembelajar bahasa Inggris sebagai bahasa asing di tingkat sekolah menengah pertama (Suriyanti & Yaacob, 2016). Hal ini dikarenakan kegiatan menulis yang menuntut pengorganisasian ide yang baik sehingga tulisan dapat terbaca telah menjadi tantangan tersendiri bagi siswa. Terlebih, kegiatan menulis seringkali menimbulkan kecemasan ketika dihadapkan dengan batas waktu namun siswa dituntut jeli dalam pemilihan kata, penggunaan grammar yang benar hingga koherensi konten. Oleh karena itu,  terlepas dari kompleksitas yang ada pada kegiatan menulis tersebut, model pembelajaran sangat berperan penting dalam membantu siswa keluar dari kesulitan kesulitan tersebut. Salah satu model pembelajaran yang cocok untuk diterapkan yaitu model pembelajaran Genre Based Approach. Model Pembelajaran Genre Based Approach sangat memudahkan siswa dalam menulis, dimana model ini akan memandu siswa dengan empat tahapan menulis seperti building the field of knowledge atau kegiatan membangun konteks, modeling or deconstructing the genre atau menelaah model/ dekonstruksi teks, joint construction atau latihan terbimbing dan independent writing atau unjuk kerja mandiri.
PENERAPAN TEKNOLOGI INTEGRATING FISH AND PLANT CULTURE UNTUK MENINGKATKAN KETAHANAN PANGAN KELUARGA PADA MASA PANDEMIC WABAH COVID-19 DI DESA CAHAYA NEGERI KABUPATEN SELUMA
Authors: Hamdani Dedy , Johan Henny , Setiawan Iwan
Published: May 2022

Telah dilakukan kegiatan pengabdian kepada masyarakat (PPM) dengan tujuan untuk menerapkan teknologi tepat guna integrating fish and plant culture untuk meningkatkan ketahanan pangan keluarga pada masa pandemic Covid-19. Kegiatan PPM ini dilaksanakan dengan metode pelatihan, demonstrasi, dan pendampingan. Pelaksanaan pelatihan dibagi menjadi 2 sesi yaitu sesi pertama pemaparan materi dengan menggunakan metode ceramah, demonstrasi, dan diskusi. Sesi ke dua pendampingan kepada untuk menerapkan teknologi tersebut. Instrument yang digunakan dalam kegiatan PPM ini adalah angket untuk menjaring data respon peserta terhadap kegiatan pelatihan yang telah dilaksanakan.  Kegiatan pelatihan ini melibatkan 15 orang warga dari desa bukit peninjauan 1 cahaya negeri dengan tetap mematuhi protocol klesehatan. Jumlah peserta dibatasi mengingat kita harus menerapkan social distancing dan menghindari kerumunan. Perkembangan ikan lele dan sayuran diukur melalui observasi langsung. Berdasarkan hasil kegiatan pelatihan diketahui bahwa media untuk menerapkan teknologi integrating fish and plant culture berhasil dibuat. Berdasarkan hasil angket respon diketahu bahwa rata-rata 93% dari total peserta sangat setuju bahwa kegiatan pelatihan yag telah dilakukan memberikan banyak manfaat khusus nya untuk pemenuhan kebutuhan pangan keluarga terutama dimasa pandemic. 87% dari total peserta pelatihan menyatkan bahwa mereka dapat memahami dengan baik materi yang telah disampaikan. Peserta pelatihan menyatakan bahwa teknologi yang diajarkan didalam kegiatan pelatihan mudah untuk diterapkan secara mandiri dengan biaya yang relative rendah dan pemeliharaan yang lebih mudah. Berdasarkan hasil observasi, pertumbuhan ikan lele dalam media ember dan pertumbuhan sayuran dalam media netpot relative sangat baik. Berdasarkan hasil dan pembahsan dapat disimpulkan bahwa teknologi tepat guna berupa integrating fish and plant culture dapat menjadi solusi permasalahan ketahanan pangan keluarga dimasa pandemic covid-19 karena mudah untuk diterapkan, biaya reatif murah, pemeliharaan yang relative mudah.
Pelatihan Pengembangan Bahan Ajar Keterampilan Membaca Berbasis Konten Keislaman Untuk Guru Bahasa Inggris di Tingkat Madrasah Aliya (MA) Kota Bengkulu
Authors: Martina Feny , Friantary Heny , Syafryadin Syafryadin
Published: May 2022

Sebagai media pendukung siswa dalam belajar, bahan ajar memegang peranan yang sangat penting. Bahan ajar merupakan sumber belajar yang digunakan guru untuk mencapai tujuan pembelajaran. Oleh karenanya, bahan ajar hendaklah memiliki kualitas yang baik. Baik tidaknya kualiatas bahan ajar sangat menentukan mutu pembelajaran. Bahan ajar berkualitas tentunya dibuat dan dikembangkan sesuai kebutuhan guru dan siswa. Artikel ini menyajikan pelatihan pengembangan bahan ajar keterampilan membaca (reading skills) yang dilakukan kepada guru bahasa Inggris  di selingkupan Madrasah Aliya (MA) Kota Bengkulu. Menimbang siswa MA memiliki kebutuhan yang tidak dapat disamakan dengan siswa sekolah menengah umum lainnya, pelatihan ini menitik beratkan pada pengembangan bahan ajar keterampilan membaca Bahasa Inggris yang berbasis kebutuhan siswa MA, yakni bahan ajar yang melibatkan konten kultur dan/ atau nilai nilai yang ada pada keseharian siswa di lingkungan madrasah.  Salah satunya yakni nilai nilai keislaman. Terdapat tiga aspek nilai nilai keislaman yang difokuskan dalam pengembangan bahan ajar ini, diantaranya nilai akidah, nilai ibadah dan nilai akhlak. Ketiga nilai nilai inilah yang dicoba untuk diintegrasikan ke dalam teks reading pada produk pengembangan bahan ajar berbasis keislaman.
Endovascular parent artery coil occlusion for ruptured vertebral artery dissecting aneurysms
Authors: Puay Yong NG
Published: May 2022

Background: The outcome of ruptured vertebral artery dissecting aneurysms (VADA) managed with endovascular coil occlusion technique is reviewed. Method: This is a retrospective study of prospectively collected data of 25 cases with ruptured VADA managed by a standardized endovascular parent artery occlusion technique. All cases were treated with coil occlusion of the dilated segment with a short proximal segment of the involved non-dominant or co-dominant vertebral artery within 12 hours of admission. All cases were done under general anesthesia with no anticoagulation. Outcome was assessed clinically with modified Rankin score as well as follow up MRI at 6 months. Results: There were a total of 25 cases, 10 female and 15 males. Age range 18 to 70, mean age 41. Twenty cases (80%) were WFNS grade 1 to 3. Five cases were grade 4 to 5. Treatment complication of cerebellar embolic infarct occurred in one case (4%). There was no hemorrhagic complication post treatment. 16 cases required ventriculoperitoneal shunting. Outcome was good with modified Rankin score of 0 to 2 in 20 cases (80%). There were 5 poor outcomes including one death (4%) due to the primary effect of subarachnoid hemorrhage in a case presenting with WFNS grade 5. All cases with WFNS grade 1 to 3 at presentation obtained good outcome. In all 24 cases who survived, follow up MRI showed that all aneurysms remained occluded at 6 months. Conclusion: Endovascular parent artery coil occlusion is effective and durable for ruptured VADA.
The management of newborns of mothers who are COVID-19 positive in a "COVID-free" hospital in Italy
Authors: Claudio, Migliori , Marta Braga , Cristina, Villa Maria , Aristide Cotta-Ramusino , Stefano Bianchi
Published: May 2022

The strategies planned by the Italian Ministry of Health to manage the COVID-19 pandemic include the centralisation of positive subjects in specific centres (Hubs) as well keeping others "COVID free". However due to the large number of positive subjects, the beds available have sometimes been inadequate. All pregnant women, at admission to the hospital, are screened and, if positive, sent to an Hub, but in the case of known positivity after birth, without bed availability, we structured a plan to complete the hospitalisation in our department of asymptomatic mothers with negative newborns. From October to December 2020, eleven babies were born to mothers who tested COVID-19-positive after delivery. Seven of them completed hospitalisation in our department. No complications in mother-baby dyads and no contagion among hospital staff were found. This strategy, even in a limited number of cases, allowed the management without complications for both patients and healthcare professionals.
Left atrial appendage closure with device for atrial fibrillation
Authors: Massimo Benedetto , Giovanni Fazio
Published: May 2022

Atrial fibrillation is the most common observed arrhythmia in clinical practice and it represents an important risk factor for cardio-embolism and cardio-embolic stroke. Atrial fibrillation patients have a 5 fold increase in stroke events. In non-valvular atrial fibrillation over 90% of thrombus formation is within the left atrial appendage and therefore prevention of embolization to systemic circulation and in particular to cerebral circulation goes through a therapy that has the left atrial appendage as the main target. Several anatomo-pathological studies and observations during open heart surgery confirm these data and give a rational basis to this point of view.
Comparison of ACR-TIRADS to the ATA Guidelines for Thyroid Nodules: A Neck to Neck Comparison
Authors: Qiang, Judy K ,   1 Department of Medicine, Division of Endocrinology and Metabolism, University of Toronto; Toronto, Ontario, Canada, Betel Carrie ,   2 Department of Medical Imaging, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada, Hack Kalesha , Ghorab Zeina , Gilmour Julie , Mohammadi Manijeh , Burton Kirsteen , Higgins Kevin M , Halperin Ilana
Published: May 2022

Introduction: The goal of this study was to compare the performance characteristics of the American College of Radiology Thyroid Imaging Reporting and Data System (ACR-TIRADS) and the American Thyroid Association (ATA) systems in identifying malignant thyroid nodules. Methods: In a retrospective chart review, ultrasound images of all thyroid nodules biopsied in 2014- 2015 at a Canadian academic centre were reviewed by two radiologists. The ultrasound characteristics of thyroid nodules were compared with cytologic or pathologic results to determine the positive predictive value (PPV), negative predictive value (NPV), sensitivity and specificity for TIRADS and ATA in predicting cancer risk. Clinical course of nodules not requiring follow up or intervention according to ACR-TIRADS was described. Vascularity was added to ACR-TIRADS to determine whether sensitivity of TIRADS improves. Results: A total of 417 thyroid nodules were reviewed, 82% were benign (Bethesda II). The sensitivity, specificity, PPV, and NPV were 97%, 11%, 9%, 98%, and 70%, 29%, 18%, and 81% for ATA and TIRADS, respectively. Of the 10 nodules that did not need ultrasound follow up based on TIRADS criteria, 2 were malignant, the rest were FLUS. If vascularity was added to TIRADS (TIRADS-Vasc), the number of malignant cases missed could have been reduced by 43% (from 7 to 4 cases). Conclusions: TIRADS is more specific but less sensitive than ATA, and misses a small number of malignant nodules. Clinicians need to use their judgement to decide which nodules require biopsy as some malignant cases will be missed using TIRADS alone.
Immunohistochemical Analysis of Laryngeal Lesions and Clinical-Laryngoscopic Correlation
Authors: Rimoli Caroline Fernandes , Hamerschmidt Rogério , Dutra Júlia Rodrigues Marcondes , de Macedo Filho Evaldo Dacheux , Pauna Henrique Furlan
Published: May 2022

Introduction: The vocal fold is vulnerable to several types of lesions. Immunohistochemistry is a technique aimed to complement the routine histopathological investigation, showing the presence of different antigens in the tissue or cell by means of specific antibodies, thus allowing the assessment of the potential proliferation of neoplastic cells. Objective: Detailed review of the results of vocal fold biopsies and correlation between histopathological data and clinical course among patients. Methods: A descriptive retrospective study based on the analysis of medical records of patients undergoing laryngeal microsurgery due to benign diseases (polyps and nodules), whose biopsies required additional investigation using immunohistochemistry techniques. All biopsies were carried out by the same specialist, from January 2018 to December 2019 at the Hospital Paranaense de Otorrinolaringologia. Results: Seven out of the 29 patients included in this study (26 male and 3 female patients) claimed to be tobacco users. All the 18 patients investigated for high-risk HPV scored negative in diagnostic immunohistochemistry. Three of the 19 patients with suspected laryngeal papillomatosis resulted in squamous cell carcinoma in the anatomopathological examination. Conclusion: Due to similar histopathological aspects between the different types of vocal fold lesions, there is considerable doubt regarding the gold standard in histopathological examination. To determine the difference between benign lesions of the larynx, this paper reinforce the major role of performing immunohistochemistry examinations in the diagnosis of apparently benign lesions regarding early diagnosis and treatment.
Simulation-based training using a novel Surabaya hysterectomy mannequin following video demonstration to improve abdominal hysterectomy skills of obstetrics and gynecology residents during the COVID-19 pandemic in Indonesia: a pre- and post-intervention study
Authors: Syamsuri Dara Dasawulansari , Askandar Tjokroprawiro Brahmana , Kurniawati Eighty Mardiyan , Utomo Budi , Kuswanto Djoko
Published: May 2022

Purpose: During the coronavirus disease 2019 (COVID-19) pandemic, the number of abdominal hysterectomy procedures decreased in Indonesia. The existing commercial abdominal hysterectomy simulation model is expensive and difficult to reuse. This study compared residents’ abdominal hysterectomy skills after simulation-based training using the Surabaya hysterectomy mannequin following a video demonstration.Methods: We randomized 3rd- and 4th-year obstetrics and gynecology residents to a video-based group (group 1), a simulation-based group (group 2), and a combination group (group 3). Abdominal hysterectomy skills were compared between before and after the educational intervention. The pre- and post-tests were scored by blinded experts using the validated Objective Structured Assessment of Technical Skills (OSATS) and Global Rating Scale (GRS).Results: A total of 33 residents were included in the pre- and post-tests. The OSATS and GRS mean differences after the intervention were higher in group 3 than in groups 1 and 2 (OSATS: 4.64 [95% CI, 2.90–6.37] vs. 2.55 [95% CI, 2.19–2.90] vs. 3.82 [95% CI, 2.41–5.22], P=0.047; GRS: 10.00 [95% CI, 7.01–12.99] vs. 5.18 [95% CI, 3.99–6.38] vs. 7.18 [95% CI, 6.11–8.26], P=0.006). The 3rd-year residents in group 3 had greater mean differences in OSATS and GRS scores than the 4th-year residents (OSATS: 5.67 [95% CI, 2.88–8.46]; GRS: 12.83 [95% CI, 8.61–17.05] vs. OSATS: 3.40 [95% CI, 0.83–5.97]; GRS: 5.67 [95% CI, 2.80–8.54]).Conclusion: Simulation-based training using the Surabaya hysterectomy mannequin following video demonstration can be a bridge to learning about abdominal hysterectomy for residents who had less surgical experience during the COVID-19 pandemic.
Focus and Dissolve: The Use of Cinematic Concepts in Developing Woven Textile Designs
Authors: Kate Nartker
Published: May 2022

This paper examines an interdisciplinary approach to the textile design process via two case studies of woven pattern development. Through the use of cinematic concepts and motion graphics software, woven structures are designed for textile applications. The role of experimentation and inspiration sourcing is discussed with an emphasis on new uses for unconventional technologies. Due to the commonality of creative workflows in the fields of fashion and textile design, results of this analysis have application to a range of disciplines.
A Comparative Analysis on Changing of Different Properties of Greige Woven Fabric (3/1, 2/1 Twill) After Scouring & Bleaching and Mercerizing
Authors: Mohammed Sayem Abu Naser 
Published: May 2022

The aim of the project was to compare the change of different types of physical and chemical properties like tensile strength, tear strength, GSM, EPI, absorbency and Pʰ of greige woven fabric after the pretreatment processing. That will ease the process of product development, assume the properties of the final product, and minimize textile wastage. The research included woven 2/1 and 3/1 twill fabric. Fabric samples were tested after every process except desizing. Gradual increase in EPI from greige to mercerizing of 11.75% and 20.75% in average respectively for 2/1 twill and 3/1 twill has been noted. The overall decrease in width from greige to mercerizing noted 13.09% and 16.93% respectively for 2/1 twill and 3/1 twill. GSM of the samples tended to increase after mercerizing than scouring and bleaching. Fabrics tend to have a Pʰ value around neutral after mercerizing and alkaline after scouring & bleaching. Absorbency of fabric decreases after mercerizing than scouring and bleaching. Tensile strength, Tear strength also have been tested for comparison.
PENGARUH CORPORATE IMAGE INDEX TERHADAP CITRA PERUSAHAAN PELABUHAN INDONESIA I
Authors: Thaci Hashim , Syah Dedi Kurnia
Published: May 2022

Corporate image index merupakan survei omnibus (menyeluruh) yang dilakukan dengan mengukur citra suatu perusahaan yang dapat dinilai dari empat dimensi dasar yaitu dimensi kualitas, dimensi kinerja, dimensi tanggungjawab dan dimensi daya tarik. Penelitian ini bertujuan untuk mengetahui: (1) pengaruh corporate image index terhadap citra perusahaan PT Pelabuhan Indonesia I Medan, Sumatera Utara pada tahun 2020, (2) nilai pengaruh corporate image index terhadap citra perusahaan PT. Pelabuhan Indonesia I Medan, Sumatera Utara pada tahun 2020. Penentuan sampel menggunakan purposive sampling dengan menggunakan metode eksplanatory research. Instrumen dalam penelitian ini adalah instrumen corporate image index yang terdiri dari 20 pertanyaan serta instrumen citra perusahaan yang terdiri dari 16 pertanyaan. Kedua instrumen ini dinyatakan valid dan reliabel. Uji hipotesis pada penelitian ini dengan menggunakan analisis regresi linier sederhana dan uji determinasi. Hasil pada penelitian menunjukkan: (1) terdapat pengaruh antara corporate image index terhadap citra perusahaan PT Pelabuhan Indonesia I, Medan Sumatera Utara tahun 2020, (2) besar pengaruh corporate image index terhadap citra perusahaan adalah 74,4%. Disarankan agar selanjutnya menentukan perusahaan lain yaitu perusahaan swasta dengan tujuan membandingkan tingkat keterpengaruhan variabel corporate image index antara perusahaan pemerintah dan perusahaan swasta terhadap citra perusahaan. Selain itu selanjutnya dapat diperdalam lagi dengan menggunakan metode studi kasus karena citra perusahaan menempatkan masyarakat sebagai salah satu partisipan.
Organomineral Fertilizer Is an Agronomic Efficient Alternative for Poultry Litter Phosphorus Recycling in an Acidic Ferralsol
Authors: de Melo Benites Vinicius , Dal Molin Sulian Junkes , Menezes June Faria Scherrer , Guimarães Getúlio Sousa , de Almeida Machado Pedro Luiz Oliveira
Published: May 2022

Brazil is a large phosphate importer due to the high demand for this nutrient for grain cropping on very weathered soils. However, Brazil has an important poultry production that generates ~6.8 million m3 year−1 of organic residue containing phosphorus and other nutrients, increasing the risk of environmental harm. However, if well managed, poultry litter can be a valuable source of P for crop growth in low fertile tropic soils. This article summarizes the long-term agronomic efficiency of organomineral fertilizers in supplying P to soybean and soil P maintenance as a strategy for secondary P recycling. Three fertilizers were used as P source: organomineral based on raw poultry litter, organomineral based on composted poultry litter; and a mineral monoammonium phosphate. Five soybean crop seasons were conducted with annual P doses (20, 40, 60, and 80 kg ha−1 of P2O5) and control without P application. After five crops, one additional soybean crop season was grown without P to evaluate the residual effect. Phosphorus concentration in leaves was evaluated in the first four seasons, and soil P availability in the first, third, and fourth seasons. There was no difference in soybean grain yield, P concentration in leaves, and soil P availability as a function of the P sources in the first crop. Soybean grain yield only varied with the P rate applied after the second season, when the control had the lowest grain yield. After five crops, organomineral resulted in a slight but significant soybean yield gain compared with MAP at higher doses. Over time, the available soil P increased with the highest P dose and decreased in the control treatment. The intermediate dose of 40 kg ha−1 P2O5 was enough to keep soil P level and lead to high soybean grain yield over the time, suggesting that the rate of applied P close to the P exportation by the grains has a long-term sustainable effect even in tropical soils, where residual P stocks are used. It can be concluded that organomineral fertilizer is an efficient farm input that can be used for residue destination and nutrient recycling, especially in acid tropical soils.
Orthogonal Optimization Research on Various Nozzles of High-Speed Centrifugal Spinning
Authors: Zhang Zhiming , Liu Kang , Li Wenhui , Ji Qiaoling , Xu Qiao , Lai Zilong , Ke Changjin
Published: May 2022

High-speed centrifugal spinning is a burgeoning method of fabricating nanofibers by use of the centrifugal force field. This article studied four different spinning nozzles, which were called stepped nozzle, conical-straight nozzle, conical nozzle, and curved-tube nozzle, to explore the optimal nozzle structures for fabricating nanofibers. According to the principle of centrifugal spinning, the spinning solution flow states within the four nozzles were analyzed, and the solution outlet velocity model was established. Then, the structural parameters of the four kinds of nozzles were optimized with the spinning solution outlet velocity as the test index by combining the orthogonal test and numerical simulation. Based on the orthogonal test results, the influence of nozzle structure parameters on the solution outlet velocity was analyzed, and the best combination of parameters of the centrifugal spinning nozzle structure was obtained. Subsequently, the four kinds of nozzles were used to fabricate nanofibers in the laboratory, under different solution concentration, motor rotation speed, and outlet diameters. Finally, the scanning electron microscope (SEM) was applied to observe the morphology and surface quality of nanofibers. It was found that the surface of nanofibers manufactured by the conical-straight nozzle and curved-tube nozzle was smoother than that by stepped and conical nozzles, and the fiber diameter by the conical-straight nozzle was minimal, followed by curved-tube nozzles, stepped nozzles, and conical nozzles in the diameter distribution of nanofibers.
Optimization and Kinetic Modeling of a Fed-Batch Fermentation for Mannosylerythritol Lipids (MEL) Production With Moesziomyces aphidis
Authors: Beck Alexander , Vogt Franziska , Hägele Lorena , Rupp Steffen , Zibek Susanne
Published: May 2022

Mannosylerythritol lipids are glycolipid biosurfactants with many interesting properties. Despite the general interest in those molecules and the need for a robust process, studies on their production in bioreactors are still scarce. In the current study, the fermentative production of MEL in a bioreactor with Moesziomyces aphidis was performed using a defined mineral salt medium. Several kinetic process parameters like substrate consumption rates and product formation rates were evaluated and subsequently enhanced by increasing the biomass concentration through an exponential fed-batch strategy. The fed-batch approaches resulted in two to three fold increased dry biomass concentrations of 10.9–15.5 g/L at the end of the growth phase, compared with 4.2 g/L in the batch process. Consequently, MEL formation rates were increased from 0.1 g/Lh up to around 0.4 g/Lh during the MEL production phase. Thus, a maximum concentration of up to 50.5 g/L MEL was obtained when oil was added in excess, but high concentrations of residual fatty acids were also present in the broth. By adjusting the oil feeding to biomass-specific hydrolysis and MEL production rates, a slightly lower MEL concentration of 34.3 g/L was obtained after 170 h, but at the same time a very pure crude lipid extract with more than 90% MEL and a much lower concentration of remaining fatty acids. With rapeseed oil as substrate, the ideal oil-to-biomass ratio for full substrate conversion was found to be around 10 goil/gbiomass. In addition, off-gas analysis and pH trends could be used to assess biomass growth and MEL production. Finally, kinetic models were developed and compared to the experimental data, allowing for a detailed prediction of the process behavior in future experiments.
Integrative Analysis of KCNK Genes and Establishment of a Specific Prognostic Signature for Breast Cancer
Authors: Zou Yutian , Xie Jindong , Tian Wenwen , Wu Linyu , Xie Yi , Huang Shanshan , Tang Yuhui , Deng Xinpei , Wu Hao , Xie Xinhua
Published: May 2022

Two-pore domains potassium channel subunits, encoded by KCNK genes, play vital roles in breast cancer progression. However, the characteristics of most KCNK genes in breast cancer has yet to be clarified. In this study, we comprehensively analyzed the expression, alteration, prognosis, and biological functions of various KCNKs in breast cancer. The expression of KCNK1/4/6/9/10/13 were significantly upregulated, while KCNK2/3/5/7/17 were downregulated in breast cancer tissues compared to normal mammary tissues. Increased expression of KCNK1/3/4/9 was correlated with poor overall survival, while high expression of KCNK2/7/17 predicted better overall survival in breast cancer. Eight KCNK genes were altered in breast cancer patients with a genomic mutation rate ranged from 1.9% to 21%. KCNK1 and KCNK9 were the two most common mutations in breast cancer, occurred in 21% and 18% patients, respectively. Alteration of KCNK genes was associated with the worse clinical characteristics and higher TMB, MSI, and hypoxia score. Using machine learning method, a specific prognostic signature with seven KCNK genes was established, which manifested accuracy in predicting the prognosis of breast cancer in both training and validation cohorts. A nomogram with great predictive performance was afterwards constructed through incorporating KCNK-based risk score with clinical features. Furthermore, KCNKs were correlated with the activation of several tumor microenvironment cells, including T cells, mast cells, macrophages, and platelets. Presentation of antigen, stimulation of G protein signaling and toll-like receptor cascaded were regulated by KCNKs family. Taken together, KCNKs may regulate breast cancer progression via modulating immune response which can serve as ideal prognostic biomarkers for breast cancer patients. Our study provides novel insight for future studies evaluating their usefulness as therapeutic targets.
The Regulatory Roles of Chemerin-Chemokine-Like Receptor 1 Axis in Placental Development and Vascular Remodeling During Early Pregnancy
Authors: Zhang Qingqing , Xiao Zhonglin , Lee Cheuk-Lun , Duan Yong-Gang , Fan Xiujun , Yeung William S. B. , Chiu Philip C. N. , Zhang Jian V.
Published: May 2022

Chemerin is an adipokine that regulates metabolism in pregnancy. An elevation of serum chemerin level is associated with pregnancy complications. Consistently, we demonstrated that the chemerin expression was increased in placenta of preeclamptic patients at deliveries. The G protein-coupled receptor chemokine-like receptor 1 (CMKLR1) mediates the actions of chemerin. The functions of the chemerin-CMKLR1 axis in maintaining pregnancy are still unknown. In this study, we demonstrated that CMKLR1 was expressed in the decidual natural killer (dNK) cells and chorionic villi of human. Chemerin suppressed the proliferation of the dNK cells in vitro. Specific antagonist of CMKLR1, α-Neta abolished the suppressive effect of spent medium from chemerin-treated dNK cells culture on extravillous trophoblast invasion. Activation of the chemerin-CMKLR1 axis promoted fusion and differentiation of human cytotrophoblast to syncytiotrophoblast in vitro. We generated Cmklr1 knockout mice and showed that the Cmklr1 deficiency negatively affected pregnancy outcome in terms of number of implantation sites, litter size and fetal weight at birth. Histologically, the Cmklr1 deficiency impaired formation of the syncytiotrophoblast layer II, induced enlargement of the maternal lacunae in the labyrinth, increased the diameter of the spiral arteries and increased trophoblast invasion in the decidua. The Cmklr1 deficient placenta also displayed an increased number of dNK cells and serum IL-15 level. In summary, the chemerin-CMKLR1 axis regulated placental development and spiral artery remodeling in early pregnancy.
Peroxisome-Derived Hydrogen Peroxide Modulates the Sulfenylation Profiles of Key Redox Signaling Proteins in Flp-In T-REx 293 Cells
Authors: Lismont Celien , Revenco Iulia , Li Hongli , Costa Cláudio F. , Lenaerts Lisa , Hussein Mohamed A. F. , De Bie Jonas , Knoops Bernard , Van Veldhoven Paul P. , Derua Rita , Fransen Marc
Published: May 2022

The involvement of peroxisomes in cellular hydrogen peroxide (H2O2) metabolism has been a central theme since their first biochemical characterization by Christian de Duve in 1965. While the role of H2O2 substantially changed from an exclusively toxic molecule to a signaling messenger, the regulatory role of peroxisomes in these signaling events is still largely underappreciated. This is mainly because the number of known protein targets of peroxisome-derived H2O2 is rather limited and testing of specific targets is predominantly based on knowledge previously gathered in related fields of research. To gain a broader and more systematic insight into the role of peroxisomes in redox signaling, new approaches are urgently needed. In this study, we have combined a previously developed Flp-In T-REx 293 cell system in which peroxisomal H2O2 production can be modulated with a yeast AP-1-like-based sulfenome mining strategy to inventory protein thiol targets of peroxisome-derived H2O2 in different subcellular compartments. By using this approach, we identified more than 400 targets of peroxisome-derived H2O2 in peroxisomes, the cytosol, and mitochondria. We also observed that the sulfenylation kinetics profiles of key targets belonging to different protein families (e.g., peroxiredoxins, annexins, and tubulins) can vary considerably. In addition, we obtained compelling but indirect evidence that peroxisome-derived H2O2 may oxidize at least some of its targets (e.g., transcription factors) through a redox relay mechanism. In conclusion, given that sulfenic acids function as key intermediates in H2O2 signaling, the findings presented in this study provide valuable insight into how peroxisomes may be integrated into the cellular H2O2 signaling network.
Application of CRISPR/Cas9 System in Establishing Large Animal Models
Authors: Lin Yingqi , Li Jun , Li Caijuan , Tu Zhuchi , Li Shihua , Li Xiao-Jiang , Yan Sen
Published: May 2022

The foundation for investigating the mechanisms of human diseases is the establishment of animal models, which are also widely used in agricultural industry, pharmaceutical applications, and clinical research. However, small animals such as rodents, which have been extensively used to create disease models, do not often fully mimic the key pathological changes and/or important symptoms of human disease. As a result, there is an emerging need to establish suitable large animal models that can recapitulate important phenotypes of human diseases for investigating pathogenesis and developing effective therapeutics. However, traditional genetic modification technologies used in establishing small animal models are difficultly applied for generating large animal models of human diseases. This difficulty has been overcome to a great extent by the recent development of gene editing technology, especially the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). In this review, we focus on the applications of CRISPR/Cas9 system to establishment of large animal models, including nonhuman primates, pigs, sheep, goats and dogs, for investigating disease pathogenesis and treatment. We also discuss the limitations of large animal models and possible solutions according to our current knowledge. Finally, we sum up the applications of the novel genome editing tool Base Editors (BEs) and its great potential for gene editing in large animals.
Bioelectrochemical Treatment Technology—The New Practical Approach for Wastewater Management and GHG Emissions Reduction
Authors: Babanova Sofia , Jones Jason , Wiseman Kelly , Soles Jaime , Garcia Jaime , Huerta Pedro , Barocio Daniel , Naito Ryoji , Arreola Orlando , Garcia Gerardo , Bretschger Orianna
Published: May 2022

This study presents BioElectrochemical Treatment Technology (BETT) as a new wastewater management solution toward the Net-Zero future. The results reported herein were collected from a BETT pilot system installed at a large brewery in Los Angeles, CA, United States processing 0.6 m3. day-1 of raw brewery wastewater with a high content of fruit pulp. Removal of Chemical Oxygen Demand (COD), Total Suspended Solids (TSS) and protein in mg.L-1 per day or percentage were evaluated over 2 months of continuous operation of the Demo Unit. The GHG emissions associated with the power consumed, biomass produced, and carbon dioxide emitted were estimated and compared to aerobic and anaerobic solutions. It was demonstrated that BETT can process wastewater with higher organic load than most conventional anaerobic systems. The inflow COD loading varied between 48,550 mg/L to 116,200 mg/L, and BETT achieved up to 33% COD removal in 4-h HRT. The TSS removal reached values as high as 79% with incoming TSS concentrations up to 34,000 mg/L TSS. BETT did not directly generate methane and demonstrated 89 and 49% lower landfill methane emissions than aerobic and anaerobic technologies, respectively. The overall reduction in CO2 emissions, both direct and indirect, was estimated to be 85–90% compared to existing practices.
High Performance of Functionalized Graphene Hydrogels Using Ethylenediamine for Supercapacitor Applications
Authors: Ju Hong , Xu Weihui , Fang Lu , Duan Jinzhuo
Published: May 2022

High-performance supercapacitor (SC) electrodes typically require excellent rate capabilities, long cycle life, and high energy densities. In this work, ethylenediamine (EDA) functionalized graphene hydrogels (FGHs) with a high capacitor performance were prepared from graphene oxide (GO) dispersions using a two-step hydrothermal method. In addition, we used a very small amount of EDA to achieve the partial reduction and functional modification of GO, and the synthesized FGH-4 binder-free electrodes exhibited a high specific capacitance of −240 F/g at 1 A/g. We also successfully fabricated a symmetric SC device based on the FGH-4 electrode, with a wide voltage window of 3.0 V. More importantly, the as-assembled symmetric SC delivered a high specific energy of 39 Wh/kg at a specific power of 749 W/kg, while still maintaining its superior cycle life (retaining 88.09% of its initial capacitance after 10,000 cycles).
New Sesquiterpenoids From Plant-Associated Irpex lacteus
Authors: Luo Huai-Zhi , Jiang Huan , Huang Xi-Shan , Jia Ai-Qun
Published: May 2022

Bacteria produce a large number of virulence factors through the quorum sensing (QS) mechanism. Inhibiting such QS system of the pathogens without disturbing their growth is a potential strategy to control multi-drug-resistant pathogens. To accomplish this, two new tremulane-type sesquiterpenoids, irpexolaceus H (1) and I (2), along with two known furan compounds, irpexlacte B (3) and C (4), were isolated from Orychophragmus violaceus (L.) OE Schulz endophytic fungus Irpex lacteus (Fr.) Fr. Their structures were elucidated by detailed spectroscopic data (NMR, HRESIMS, IR, and UV), single-crystal X-ray diffraction, and electronic circular dichroism (ECD) analysis. Furthermore, those compounds were evaluated for anti-quorum sensing (anti-QS) activity, and compound 3 was found contributing to the potential QS inhibitory activity.
Gut Microbiome Characteristics in IgA Nephropathy: Qualitative and Quantitative Analysis from Observational Studies
Authors: Han Shisheng , Shang Li , Lu Yan , Wang Yi
Published: May 2022

Background

Recent data indicate the importance of gut-kidney axis in the pathogenesis of Immunoglobulin A nephropathy (IgAN). Growing evidence suggests the alterations of diversity and composition of gut microbiome among patients with IgAN, however, the details are not yet fully understood.

Methods

Eligible studies comparing the gut microbiome between patients with IgAN and non-IgAN individuals were systematically searched from PubMed, Embase, Web of Science, Cochrane Library, China National Knowledge Infrastructure, and ClinicalTrials.gov. The primary outcomes were alpha- and beta-diversity, and the differences in gut microbiota composition between patients with IgAN and non-IgAN persons. Qualitative analysis and meta-analysis were performed according to available data.

Results

Eleven cross-sectional studies, including 409 patients with IgAN and 243 healthy controls, were enrolled. No significant differences in the diversity and enrichment of gut bacteria were found between IgAN and healthy individuals, whereas the beta-diversity consistently showed significant microbial dissimilarities among the two groups. Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Fusobacteria, and Verrucomicrobia were the dominant phyla, however, no significant differences were found between IgAN patients and healthy controls at the phylum level. The genera, Streptococcus and Paraprevotella showed a higher proportion in patients with IgAN compared to healthy individuals, whereas Fusicatenibacter showed a lower abundance according to meta-analysis. Qualitative analyses suggested that Escherichia-Shigella might be increased in IgAN patients; the genera, Clostridium, Prevotella 9,and Roseburia, members of Ruminococcaceae and Lachnospiraceae families, were likely to have decreased abundances in patients with IgAN compared to healthy individuals.

Conclusion

Gut microbiota dysbiosis was demonstrated in IgAN, which might be involved in the pathogenesis of IgAN. Further studies are needed to confirm the findings of this study, due to the substantial heterogeneity.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier PROSPERO (CRD42022304034).
Enhanced Weathering Using Basalt Rock Powder: Carbon Sequestration, Co-benefits and Risks in a Mesocosm Study With Solanum tuberosum
Authors: Vienne Arthur , Poblador Silvia , Portillo-Estrada Miguel , Hartmann Jens , Ijiehon Samuel , Wade Peter , Vicca Sara
Published: May 2022

Enhanced weathering (EW) of silicate rocks can remove CO2 from the atmosphere, while potentially delivering co-benefits for agriculture (e.g., reduced nitrogen losses, increased yields). However, quantification of inorganic carbon sequestration through EW and potential risks in terms of heavy metal contamination have rarely been assessed. Here, we investigate EW in a mesocosm experiment with Solanum tuberosum growing on alkaline soil. Amendment with 50 t basalt/ha significantly increased alkalinity in soil pore water and in the leachate losses, indicating significant basalt weathering. We did not find a significant change in TIC, which was likely because the duration of the experiment (99 days) was too short for carbonate precipitation to become detectable. A 1D reactive transport model (PHREEQC) predicted 0.77 t CO2/ha sequestered over the 99 days of the experiment and 1.83 and 4.48 t CO2/ha after 1 and 5 years, respectively. Comparison of experimental and modeled cation pore water Mg concentrations at the onset of this experiment showed a factor three underestimation of Mg concentrations by the model and hence indicates an underestimation of modeled CO2 sequestration. Moreover, pore water Ca concentrations were underestimated, indicating that the calcite precipitation rate was overestimated by this model. Importantly, basalt amendment did not negatively affect potato growth and yield (which even tended to increase), despite increased Al availability in this alkaline soil. Soil and pore water Ni increased upon basalt addition, but Ni levels remained below regulatory environmental quality standards and Ni concentrations in leachates and plant tissues did not increase. Last, basalt amendment significantly decreased nitrogen leaching, indicating the potential for EW to provide benefits for agriculture and for the environment.