Effect of novel IL-8 gene mutation on its protein structure and stability among ovarian cancer patients in Saudi Arabia
Authors:   King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia, Haque Absarul ,   Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia, Ali Baig Ghazanfar ,   Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia, Alshawli Abdulelah Saleh ,   King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia,   Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia, Wali Sait Khalid Hussain ,   Department of Gynecology Oncology Unit, Obstetrics and Gynecology, Faculty of Medicine, King Abdulaziz University Hospital, Jeddah, Saudi Arabia, Mohammed Ali Hani S.H ,   Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia, Hussain Sait Hesham Khalid ,   Department of Gynecology Oncology Unit, Obstetrics and Gynecology, Faculty of Medicine, King Abdulaziz University Hospital, Jeddah, Saudi Arabia, Hussain Sait Hesham Khalid ,   Department of Gynecology Oncology Unit, Obstetrics and Gynecology, Faculty of Medicine, King Abdulaziz University Hospital, Jeddah, Saudi Arabia, Bibi Fehmida ,   Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia,   Special Infectious Agents Unit, King Fahd Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia, Pushparaj Peter Natesan ,   Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia,   Institute of Genomic Medicine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia, Rasool Mahmood ,   Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia,   Institute of Genomic Medicine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia, Naseer Muhammad Imran ,   Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia,   Institute of Genomic Medicine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
Published: February 2025

Despite advancements in therapeutics, early diagnosis of ovarian cancer (OC) remains a challenge due to its asymptomatic progression and it is often diagnosed at an advanced stage. Interleukin-8 (IL-8), a pro-inflammatory chemokine with angiogenic activity, plays a pivotal role in inflammation and carcinogenesis. Therefore, it is of interest to investigate the association between IL-8 polymorphisms and OC risk. A novel non-synonymous SNP (nsSNP, c.193G>A; p.Glu65Leu) was identified in exon 2 of the IL-8 gene in 5 patients, 3 with stage III and 2 with stage II tumor. Structural analysis suggested that the p.Glu65Leu mutation alters the IL 8 protein conformation showing functional impairment. Thus, data shows that IL-8 c.193 G>A (Glu65Leu) polymorphism may contribute to tumor susceptibility. However, further studies are warranted to understand the role of IL-8 polymorphism in OC.