bluuebunny/crossref_metadata_2025 huggingface dataset:
Energy Efficiency and Load Optimization in Heterogeneous Networks through Dynamic Sleep Strategies: A Constraint-Based Optimization Approach
Authors: Shabbir Amna , Shirazi Muhammad Faizan , Rizvi Safdar , Ahmad Sadique , Ateya Abdelhamied A.
Published: July 2024

This research endeavors to advance energy efficiency (EE) within heterogeneous networks (HetNets) through a comprehensive approach. Initially, we establish a foundational framework by implementing a two-tier network architecture based on Poisson process distribution from stochastic geometry. Through this deployment, we develop a tailored EE model, meticulously analyzing the implications of random base station and user distributions on energy efficiency. We formulate joint base station and user densities that are optimized for EE while adhering to stringent quality-of-service (QoS) requirements. Subsequently, we introduce a novel dynamically distributed opportunistic sleep strategy (D-DOSS) to optimize EE. This strategy strategically clusters base stations throughout the network and dynamically adjusts their sleep patterns based on real-time traffic load thresholds. Employing Monte Carlo simulations with MATLAB, we rigorously evaluate the efficacy of the D-DOSS approach, quantifying improvements in critical QoS parameters, such as coverage probability, energy utilization efficiency (EUE), success probability, and data throughput. In conclusion, our research represents a significant step toward optimizing EE in HetNets, simultaneously addressing network architecture optimization and proposing an innovative sleep management strategy, offering practical solutions to maximize energy efficiency in future wireless networks.
Comparison of Nutritional Quality and Functional Active Substances in Different Parts of Eight Lotus Seed Cultivars
Authors: Liu Xueting , Dong Wanyu , Yi Yang , Wang Limei , Hou Wenfu , Ai Youwei , Wang Hongxun , Min Ting
Published: July 2024

In this study, “Honghu White Lotus”, “Red Lotus (HH)”, “Hunan Cunshan Lotus (CS)”, “Wuyi Xuanlian”, “Space Lotus 36”, “Fujian Jianning White Lotus (JB)”, “Jiangsu Yangzhou Lotus (JY)”, and “Suzhou Dongshan Lotus” were selected as experimental subjects. The lotus seed flesh and lotus plumule of each cultivar were selected for nutritional quality and functional active substance analyses. Comparing different cultivars of lotus seeds, the protein and crude fat contents of JY flesh were the highest at 65.59 mg/g and 13%, respectively. The VC content of JB flesh and lotus plumule is the highest at 41.56 mg/g and 204.29 mg/g, respectively. JB flesh has the lowest soluble sugar content, at 17.87 mg/g, while HB’s lotus plumule and flesh have the highest content, at 33.67 mg/g and 29.62 mg/g, respectively. There was no significant difference in the crude fat content of the flesh and lotus plumule among the eight cultivars. TK flesh and lotus plumule have the highest amylose content, at 23.67 mg/g and 76.81 mg/g, respectively. Among them, the total starch content of JB (476.17 mg/g) was relatively high, whereas its amylose content was only 26.09 mg/g. Lower amylose content makes it less prone to aging. The total phenolic and flavonoid contents of the JY lotus plumule were the highest, at 18.64 and 21.04 mg/g, respectively. The alkaloid content of CS, HH, and JY was relatively high at 20.01, 19.29, and 18.68 mg/g, respectively. These can provide a consultation for the estimation and processing of the nutritional quality of different lotus seeds.
A Preliminary Study on the Effect of Adding Sugarcane Syrup on the Flavor of Barley Lager Fermentation
Authors: Lv Hechao , Jia Yusheng , Liu Chaoyi , Xu Jia , Xie Caifeng , Li Kai , Huang Kai , Hang Fangxue
Published: July 2024

This study focuses on the diversified utilization of the sugarcane industry, and sugarcane syrup, as a by-product of the sugarcane industry, is a good raw material for fermentation. Bringing sugarcane syrup into beer is conducive to the enrichment of the sugar industry, and it can improve the flavor of beer and make it more aromatic. This study determined the optimal fermentation process for beer. By analyzing the consumption rate of the carbon and nitrogen sources of raw materials, the nutrient utilization of yeast, and the causes of differences in flavor substances, the flavor composition and flavor stability of beer were determined by SPME-HS-GC-MS technology. The results showed that beer brewed with sugarcane syrup as an auxiliary raw material met the basic specifications of beer. The addition of sugarcane syrup to the wort base increased the utilization of amino acids by the yeast, and LS (lager with added cane syrup) increased the nine flavor compounds of the beer, which constituted the basic flavor of the beer, bringing new flavor compounds compared with the normal all-barley beer. Forced aging experiments showed that LS produced fewer aging compounds than OWBL. Various experiments have shown that it is feasible to ferment beer with sugarcane syrup instead of partial wort.
Food from Equids—Commercial Fermented Mare’s Milk (Koumiss) Products: Protective Effects against Alcohol Intoxication
Authors: Du Ming , Liu Yuanyi , Cao Jialong , Li Xinyu , Wang Na , He Qianqian , Zhang Lei , Zhao Bilig , Dugarjaviin Manglai
Published: July 2024

Fermented mare’s milk (koumiss), a traditional Central Asian dairy product derived from fermented mare’s milk, is renowned for its unique sour taste and texture. It has long been consumed by nomadic tribes for its nutritional and medicinal benefits. This study aimed to comprehensively analyze the protective effects of koumiss against alcohol-induced harm across behavioral, hematological, gastrointestinal, hepatic, and reproductive dimensions using a mouse model. Optimal intoxicating doses of alcohol and koumiss doses were determined, and their effects were explored through sleep tests and blood indicator measurements. Pretreatment with koumiss delayed inebriation, accelerated sobering, and reduced mortality in mice, mitigating alcohol’s impact on blood ethanol levels and various physiological parameters. Histopathological and molecular analyses further confirmed koumiss’s protective role against alcohol-induced damage in the liver, stomach, small intestine, and reproductive system. Transcriptomic studies on reproductive damage indicated that koumiss exerts its benefits by influencing mitochondrial and ribosomal functions and also shows promise in mitigating alcohol’s effects on the reproductive system. In summary, koumiss emerges as a potential natural agent for protection against alcohol-induced harm, opening avenues for future research in this field.
Effects of Fermentation with Eurotium cristatum on Sensory Properties and Flavor Compounds of Mulberry Leaf Tea
Authors: Yang Xiaoyu , Liu Zijun , Zhang Yanhao , Zhao Shuangzhi , Yan Shigan , Zhu Liping , Zhou Qingxin , Chen Leilei
Published: July 2024

Mulberry leaf tea (MT) is a popular Chinese food with nutrition and medicinal functions. Solid-state fermentation with Eurotium cristatum of MT (FMT) can improve their quality. Differences in chromaticity, taste properties, and flavor characteristics were analyzed to evaluate the improvements of the sensory quality of FMT. After fermentation, the color of the tea infusion changed. The E-tongue evaluation results showed a significant decrease in unpleasant taste properties such as sourness, bitterness, astringency, and aftertaste-bitterness, while umami and saltiness taste properties were enhanced post-fermentation. Aroma-active compounds in MT and FMT were identified and characterized. A total of 25 key aroma-active compounds were screened in MT, and 2-pentylfuran showed the highest relative odor activity value (ROAV). A total of 26 key aroma-active compounds were identified in FMT, and the newly formed compound 1-octen-3-one showed the highest ROAV, which contributed to FMT’s unique mushroom, herbal, and earthy flavor attributes. 1-octen-3-one, (E)-2-nonenal, trimethyl-pyrazine, 2-pentylfuran, and heptanal were screened as the potential markers that contributed to flavor differences between MT and FMT. E. cristatum fermentation significantly altered the sensory properties and flavor compounds of MT. This study provides valuable insights into the sensory qualities of MT and FMT, offering a theoretical basis for the development of FMT products.
The Light-Intensity-Affected Aroma Components of Green Tea during Leaf Spreading
Authors: He Youyue , Liu Shujing , Kang Yuzhong , Periakaruppan Rajiv , Zhuang Jing , Wang Yuhua , Chen Xuan , Liu Xinqiu , Li Xinghui
Published: July 2024

Leaf spreading is a key processing step that affects the aroma formation of green tea. The effects of a single-light wavelength on the aroma and taste of tea have been extensively studied. Less attention has been paid to the effect of different complex light intensities on the formation of green tea’s volatile aroma during leaf spreading. The current study was designed to evaluate how leaf spreading under different complex light intensities relates to the quality of green tea. Using headspace solid-phase micro-extraction and gas chromatography-mass spectrometry (HS-SPME/GC-MS), volatile flavor compounds in green tea were analyzed during leaf spreading in five different light conditions. Multivariate statistical analysis and odor activity values (OAVs) were used to classify these samples and identify key odors. Eight distinct groups, including ninety volatile compounds, were detected. The most prevalent volatile compounds found in green tea samples were hydrocarbons and alcohols, which accounted for 29% and 22% of the total volatile compounds, respectively. Fourteen volatile compounds (OAV > 1) were identified as key active differential odorants. The chestnut-like aroma in green tea was mostly derived from 3-methyl-butanal and linalool, which were significantly accumulated in medium-intensity light (ML).
Chemiluminescent Reaction Induced by Mixing of Fluorescent-Dye-Containing Molecular Organogels with Aqueous Oxidant Solutions
Authors: Ohsedo Yutaka , Miyata Kiho
Published: July 2024

Chemiluminescence in solution-based systems has been extensively studied for the chemical analysis of biomolecules. However, investigations into the control of chemiluminescence reactions in gel-based systems, which offer flexibility in reaction conditions (such as the softness of the reaction environment), have only recently begun in polymer materials, with limited exploration in low-molecular-weight gelator (LMWG) systems. In this study, we investigated the chemiluminescence behaviors in the gel states using LMWG systems and evaluated their applicability to fluorescent-dye-containing molecular organogel systems/oxidant-containing aqueous systems. Using diethyl succinate organogels composed of 12-hydroxystearic acid as a molecular organogelator, we examined the fluorescent properties of various fluorescent dyes mixed with oxidant aqueous solutions. As the reaction medium transitioned from the solution to the gel state, the emission color and chemiluminescence duration changed significantly, and distinct characteristics were observed, for each dye. This result indicates that the chemiluminescence behavior differs significantly between the solution and gel states. Additionally, visual inspection and dynamic viscoelastic measurements of the mixed fluorescent dye-containing molecular gels and oxidant-containing aqueous solutions confirmed that the chemiluminescence induced by the mixing occurred within the gel phase. Furthermore, the transition from the solution to the gel state may allow for the modulation of the mixing degree, thereby enabling control over the progression of the chemiluminescence reaction.
Biohybrids for Combined Therapies of Skin Wounds: Agglomerates of Mesenchymal Stem Cells with Gelatin Hydrogel Beads Delivering Phages and Basic Fibroblast Growth Factor
Authors: Moghtader Farzaneh , Tabata Yasuhiko , Karaöz Erdal
Published: July 2024

There is great interest in developing effective therapies for the treatment of skin wounds accompanied by deep tissue losses and severe infections. We have attempted to prepare biohybrids formed of agglomerates of mesenchymal stem cells (MSCs) with gelatin hydrogel beads (GEL beads) delivering bacteriophages (phages) as antibacterial agents and/or basic fibroblast growth factor (bFGF) for faster and better healing, providing combined therapies for these types of skin wounds. The gelatin beads were produced through a two-step process using basic and/or acidic gelatins with different isoelectric points. Escherichia coli (E. coli) and its specific T4 phages were propagated. Phages and/or bFGF were loaded within the GELs and their release rates and modes were obtained. The phage release from the basic GEL beads was quite fast; in contrast, the bFGF release from the acidic GEL beads was sustained, as anticipated. MSCs were isolated from mouse adipose tissues and 2D-cultured. Agglomerates of these MSCs with GEL beads were formed and maturated in 3D cultures, and their time-dependent changes were followed. In these 3D culture experiments, it was observed that the agglomerates with GEL beads were very healthy and the MSCs formed tissue-like structures in 7 days, while the MSC agglomerates were not healthy and shrunk considerably as a result of cell death.
A Global Identification of Protein Disulfide Isomerases from ‘duli’ Pear (Pyrus betulaefolia) and Their Expression Profiles under Salt Stress
Authors: Zhang Hao , Zhang Yuyue , Cui Kexin , Liu Chang , Chen Mengya , Fu Yufan , Li Zhenjie , Ma Hui , Zhang Haixia , Qi Baoxiu , Xu Jianfeng
Published: July 2024

Protein disulfide isomerases (PDIs) and PDI-like proteins catalyze the oxidation and reduction in protein disulfide bonds, inhibit aggregation of misfolded proteins, and participate in isomerization and abiotic stress responses. The wild type ‘duli’ pear (Pyrus betulaefolia) is an important rootstock commonly used for commercial pear tree grafting in northern China. In this study, we identified 24 PDI genes, named PbPDIs, from the genome of ‘duli’ pear. With 12 homologous gene pairs, these 24 PbPDIs distribute on 12 of its 17 chromosomes. Phylogenetic analysis placed the 24 PbPDIs into four clades and eleven groups. Collinearity analysis of the PDIs between P. betulaefolia, Arabidopsis thaliana, and Oryza sativa revealed that the PbPDIs of ‘duli’ pear show a strong collinear relationship with those from Arabidopsis, a dicot; but a weak collinear relationship with those from rice, a monocot. Quantitative RT-PCR analysis showed that most of the PbPDIs were upregulated by salt stress. Identification and expression analysis of ‘duli’ pear PbPDIs under salt stress conditions could provide useful information for further research in order to generate salt-resistant rootstock for pear grafting in the future.
Exploring the Impact of Genetics in a Large Cohort of Moebius Patients by Trio Whole Exome Sequencing
Authors: Moresco Giada , Bedeschi Maria Francesca , Venturin Marco , Villa Roberta , Costanza Jole , Mauri Alessia , Santaniello Carlo , Picciolini Odoardo , Messina Laura , Triulzi Fabio , Miozzo Monica Rosa , Rondinone Ornella , Fontana Laura
Published: July 2024

Moebius syndrome (MBS) is a rare congenital disorder characterized by non-progressive facial palsy and ocular abduction paralysis. Most cases are sporadic, but also rare familial cases with autosomal dominant transmission and incomplete penetrance/variable expressivity have been described. The genetic etiology of MBS is still unclear: de novo pathogenic variants in REV3L and PLXND1 are reported in only a minority of cases, suggesting the involvement of additional causative genes. With the aim to uncover the molecular causative defect and identify a potential genetic basis of this condition, we performed trio-WES on a cohort of 37 MBS and MBS-like patients. No de novo variants emerged in REV3L and PLXND1. We then proceeded with a cohort analysis to identify possible common causative genes among all patients and a trio-based analysis using an in silico panel of candidate genes. However, identified variants emerging from both approaches were considered unlikely to be causative of MBS, mainly due to the lack of clinical overlap. In conclusion, despite this large cohort, WES failed to identify mutations possibly associated with MBS, further supporting the heterogeneity of this syndrome, and suggesting the need for integrated omics approaches to identify the molecular causes underlying MBS development.
Medical Genetics in Brazil in the 21st Century: A Thriving Specialty and Its Incorporation in Public Health Policies
Authors: Horovitz Dafne Dain Gandelman , Félix Têmis Maria , Ferraz Victor Evangelista de Faria
Published: July 2024

Brazil is a continent-size country with 203 million inhabitants, classified as a developing upper-middle-income country, although inequities remain significant. Most of the population is assisted by the public Unified Health System (SUS), along with a thriving private health sector. Congenital malformations are the second leading cause of infant mortality and chronic/genetic disorders and a significant burden in hospital admissions. The past two decades have been crucial for formalizing medical genetics as a recognized medical specialty in the SUS, as well as for implementing a new health policy by the Ministry of Health for comprehensive care for rare diseases. These public health policies had the broad support of the Brazilian Society of Medical Genetics and Genomics and patient organizations. Most comprehensive genetic services are concentrated in large urban centers in the South and Southeast regions of Brazil; with this new policy, new services throughout the country are progressively being integrated. The number of medical geneticists increased by 103% in a decade. Details on the policy and an overview of the availability of services, testing, human resources, newborn screening, research projects, patient organizations, and relevant issues regarding medical genetics in this vast and diverse country are presented.
Comparison of Mutations Induced by Different Doses of Fast-Neutron Irradiation in the M1 Generation of Sorghum (Sorghum bicolor)
Authors: Yuan Na , Liang Shuaiqiang , Zhou Ling , Yuan Xingxing , Li Chunhong , Chen Xin , Zhao Han
Published: July 2024

Sorghum is an important C4 crop with various food and nonfood uses. Although improvements through hybridization and selection have been exploited, the introduction of genetic variation and the development of new genotypes in sorghum are still limited. Fast-neutron (FN) mutagenesis is a very effective method for gene functional studies and to create genetic variability. However, the full spectrum of FN-induced mutations in sorghum is poorly understood. To address this, we generated an FN-induced mutant population from the inbred line ‘BTx623’ and sequenced 40 M1 seedlings to evaluate the mutagenic effects of FNs on sorghum. The results show that each line had an average of 43.7 single-base substitutions (SBSs), 3.7 InDels and 35.15 structural variations (SVs). SBSs accounted for approximately 90.0% of the total number of small mutations. Among the eight treatment groups, FN irradiation at a dose of 19 Gy generated the highest number of mutations. The ratio of transition/transversion ranged from 1.77 to 2.21, and the G/C to A/T transition was the most common substitution in all mutant lines. The distributions of the identified SBSs and InDels were similar and uneven across the genome. An average of 3.63 genes were mutated in each mutant line, indicating that FN irradiation resulted in a suitable density of mutated genes, which can be advantageous for improving elite material for one specific or a few traits. These results provide a basis for the selection of the suitable dose of mutagen and new genetic resources for sorghum breeding.
Homoeologs in Allopolyploids: Navigating Redundancy as Both an Evolutionary Opportunity and a Technical Challenge—A Transcriptomics Perspective
Authors: Aufiero Gaetano , Fruggiero Carmine , D’Angelo Davide , D’Agostino Nunzio
Published: July 2024

Allopolyploidy in plants involves the merging of two or more distinct parental genomes into a single nucleus, a significant evolutionary process in the plant kingdom. Transcriptomic analysis provides invaluable insights into allopolyploid plants by elucidating the fate of duplicated genes, revealing evolutionary novelties and uncovering their environmental adaptations. By examining gene expression profiles, scientists can discern how duplicated genes have evolved to acquire new functions or regulatory roles. This process often leads to the development of novel traits and adaptive strategies that allopolyploid plants leverage to thrive in diverse ecological niches. Understanding these molecular mechanisms not only enhances our appreciation of the genetic complexity underlying allopolyploidy but also underscores their importance in agriculture and ecosystem resilience. However, transcriptome profiling is challenging due to genomic redundancy, which is further complicated by the presence of multiple chromosomes sets and the variations among homoeologs and allelic genes. Prior to transcriptome analysis, sub-genome phasing and homoeology inference are essential for obtaining a comprehensive view of gene expression. This review aims to clarify the terminology in this field, identify the most challenging aspects of transcriptome analysis, explain their inherent difficulties, and suggest reliable analytic strategies. Furthermore, bulk RNA-seq is highlighted as a primary method for studying allopolyploid gene expression, focusing on critical steps like read mapping and normalization in differential gene expression analysis. This approach effectively captures gene expression from both parental genomes, facilitating a comprehensive analysis of their combined profiles. Its sensitivity in detecting low-abundance transcripts allows for subtle differences between parental genomes to be identified, crucial for understanding regulatory dynamics and gene expression balance in allopolyploids.
From Agni to Agency: Sita’s Liberation in Arni and Chitrakar’s Graphic Retelling of the Ramayana
Authors: Sinha Dhruvee , Ali Zeeshan
Published: July 2024

The traditional interpretations of the Ramayana have been critiqued for preserving and promoting patriarchal gender structures by emphasising masculine heroism and often portraying female characters as unidimensional symbols of selflessness, purity, and honour. This paper analyses how Samhita Arni and Chitrakar’s graphic novel Sita’s Ramayana offers a retelling that foregrounds Sita’s perspective to question and reinterpret the social constructs. By analysing the text through a feminist literary lens, this paper examines how the novel adapts the traditional narrative to provide centre stage to Sita’s various encounters with instances of oppression. The findings reveal how Arni’s retelling employs unique aesthetics that combine texts and Chitrakar’s patua art illustrations to question the traditional male-centred versions, making this novel a part of a broader structure of feminist reinterpretations that aim to highlight female agency in cultural canons. This paper examines Sita’s stance against societal expectations for women, such as self-sacrifice, while also tracking her personal growth, which is symbolically represented by her reunion with Mother Earth. The novel contributes to the ongoing tradition of literary revisionism by offering a nuanced critique of the patriarchal foundations within classical myths. This is underscored by the novel’s reinterpretation of the epic in a way that points out the plasticity of the Ramayana, which can be reshaped to support more progressive views, encouraging discourse on existing gender norms present in contemporary societies.
Breaking Boundaries: From Fall to Rapid Cardiac Rescue
Authors: D’Alonzo Michele , Piscitelli Mariantonietta , Cilia Francesco
Published: July 2024

The occurrence of pericardial rupture as a complication of blunt thoracic trauma has the potential to result in cardiac dislocation unless promptly identified and subjected to suitable surgical intervention. This phenomenon is exceptionally uncommon and is associated with a significant mortality rate between 30 and 67%. The diagnosis remains complex due to the scarcity of symptoms. However, the routine application of computed tomography (CT) scans in the management of trauma patients could facilitate the early identification of cardiac dislocation. In this case report, we described the diagnostic–therapeutic pathway of a pericardial rupture due to high-intensity trauma.
On the Identification of the a fresco or a secco Preparative Technique of Wall Paintings
Authors: Ntasi Georgia , Rossi Manuela , Alberico Miriam , Tomeo Antonella , Birolo Leila , Vergara Alessandro
Published: July 2024

The study applies both a minimal and an extended approach for a comprehensive picture of chemical components in wall paintings, including evidence of degradation. Pigments and ligands were characterized via a multi-methodological investigation, including optical microscopy, scanning electron microscopy, Raman micro-spectroscopy, GC-MS, and LC-MS/MS. Particularly, the procedure was tested on wall paintings recently excavated from a Roman domus in Santa Maria Capua Vetere. The hypothesis of a very wealthy owner is supported by the evidence of a multi-layer preparation, a rich variety of pigments, and organic ligands (both terpenic resins and animal glue). The absence of calcite in the pictorial layer (via optical and Raman microscopy) and the presence of organic binders (via GC-MS and LC-MS/MS) clearly indicates the a secco technique.
Non-Structural Carbohydrate Content and C:N:P Stoichiometry in Houpoea officinalis Flowers in Response to Development Stages
Authors: Yang Yao , Qiu Yuxian , Cheng Yu , Yu Ting , Zhu Maoyuan , Qian Wenzhang , Gao Shun , Zhuang Guoqing
Published: July 2024

Mineral elements and non-structural carbohydrates (NSCs) are important nutrients and energy sources for flower development in plants. However, no studies were reported on the dynamic changes of nutrient stoichiometry and NSC contents in Houpoea officinalis (H. officinalis) flower. In this study, the changes in carbon (C), nitrogen (N), phosphorus (P), and NSC contents as well as C:N:P stoichiometry in the pistil, stamen, and petal of H. officinalis flowers at four developmental stages were comparatively analyzed. The results showed that C, N, P, and NSC contents, as well as C:N:P stoichiometric ratios in the three parts of the flower exhibited large variations at four development stages. Development stages and organs had significant effects on the measured parameters in the three organs of H. officinalis flowers, but their interactions had no significant effects. During the flower development, C, N, and P contents in different floral parts ranged from 418.7 to 496.3 mg/g, 26.6 to 45.3 mg/g, and 0.396 to 0.656 mg/g. P content decreased continuously with development, C:N in stamen were significantly higher than those in other flower parts at the same developmental stage. Glucose, starch, fructose, and sucrose contents showed significant differences in three parts of H. officinalis flowers at four development stages. These differences may reflect differences in elemental storage capacity and biomass allocation patterns of H. officinalis flowers. In general, our data will help to improve our understanding of the relationship between NSCs and C:N:P stoichiometry in response to development stages and organs in H. officinalis flowers.
Correction: Rehal et al. A Tomato Pomace Enriched Gluten-Free Ready-to-Cook Snack’s Nutritional Profile, Quality, and Shelf Life Evaluation. Horticulturae 2022, 8, 403
Authors: Rehal Jagbir Kaur , Aggarwal Poonam , Dhaliwal Inderpreet , Sharma Meenakshi , Kaushik Prashant
Published: July 2024

The Horticulturae Editorial Office wishes to make the following changes to the author’s paper [...]
Curcuma borealis sp. nov. and C. retrocalcaria sp. nov. (Zingiberaceae): Two Novel Taxa from Northern Thailand
Authors: Saensouk Piyaporn , Saensouk Surapon , Maknoi Charun , Boonma Thawatphong
Published: July 2024

This study introduces Curcuma borealis Saensouk, P.Saensouk, and Boonma and C. retrocalcaria Saensouk, P.Saensouk, and Boonma, two newly discovered species within the Curcuma genus of the Zingiberaceae family, found in the Mae Hong Son and Chiang Mai Provinces of Northern Thailand, respectively. This research aims to clarify the taxonomic identity of these species, which were initially confused with C. ecomata Craib and C. chantaranothaii Boonma and Saensouk due to similarities in flower coloration and some morphological characteristics, respectively. Detailed morphological analysis was conducted to distinguish these species. Curcuma borealis is differentiated by its unique anthers lacking appendages or projections at the base of the spurs, in contrast to the appendages or projections present in C. ecomata, while C. retrocalcaria is characterized by anther spurs folding or curving backward under the anther base and with two purple patches at the base of the labellum, distinguishing it from C. chantaranothaii. The findings highlight distinct morphological traits that set C. borealis and C. retrocalcaria apart from existing Curcuma species. The pollen morphology of the two new taxa was also studied here for the first time. This research emphasizes the importance of thorough taxonomic scrutiny for accurate species identification and contributes to our understanding of the diversity within the Curcuma genus. Accurate identification of these species is crucial for conservation efforts, underscoring the need to protect their habitats and maintain biodiversity in the region.
Comparison of the Temperature, Radiation, and Heat Flux Distribution of a Hydrogen and a Methane Flame in a Crucible Furnace Using Numerical Simulation
Authors: Mages Alexander , Sauer Alexander
Published: July 2024

Sustainable technologies to replace current fossil solutions are essential to meet future CO2 emission reduction targets. Therefore, this paper compares key performance indicators of a hydrogen- and a methane-flame-fired crucible furnace with computational fluid dynamics simulations at identical firing powers, aiming to fully decarbonize the process. Validated numerical models from the literature were used to compare temperatures, radiation fields, radiation parameters and heat transfer characteristics. As a result, we observed higher combustion temperatures and a 19.0% higher fuel utilization rate in the hydrogen case, indicating more efficient operating modes, which could be related to the increased radiant heat flux and temperature ranges above 1750 K. Furthermore, higher scattering of the heat flux distribution on the crucible surface could be determined indicating more uneven melt bath temperatures. Further research could focus on quantifying the total fuel consumption required for the heating up of the furnace, for which a transient numerical model could be developed.
Epidemiological Features of Leptospirosis and Identification of Leptospira wolffii as a Persistently Prevailing Species in North–Central Bangladesh
Authors: Sultana Monira , Paul Shyamal Kumar , Nasreen Syeda Anjuman , Haque Nazia , Hasan Md. Kamrul , Islam Arup , Nila Sultana Shabnam , Jahan Afsana , Sathi Fardousi Akter , Hossain Tasmia , Ferdaus Syeda Jannatul , Aung Meiji Soe , Kobayashi Nobumichi
Published: July 2024

Leptospirosis is considered to be the most widespread, yet neglected, re-emerging zoonotic disease caused by infection with a pathogenic species of the genus Leptospira. Although this disease is prevalent in Bangladesh, the recent epidemiological status has not yet been well documented. In this study, we aimed to determine the prevalence of leptospirosis among febrile patients using different diagnostic methods and to characterize the epidemiological features and species of Leptospira in Mymensingh, north–central Bangladesh. Among the blood samples of 186 patients with suspected leptospirosis who met the inclusion criteria, including having a fever for more than 5 days (November 2021–June 2022), 88 samples (47%) were Leptospira-positive according to IgM LAT, IgM ELISA, or nested PCR (positivity rates: 38%, 37%, and 42%, respectively). Nested PCR showed a significantly higher positivity rate (54%) in patients with a short fever (5–10 day) than the other methods did, with lower rates among those with a longer fever. Leptospirosis cases were more common in males (68%), those 16–45 years of age (70%), residents of rural areas (81%), and farmers (41%). In addition to a fever, myalgia and jaundice were found in more than 70% of the patients, while variable symptoms were observed. The 16S rRNA sequencing analysis revealed that the Leptospira species in all the 22 samples tested were L. wolffii, belonging to the pathogenic subclade P2. This study showed the recent epidemiological features of leptospirosis in Bangladesh, indicating the presumptive predominance of L. wolffii since 2019.
Sociocultural Implications in the Development of Early Maladaptive Schemas in Adolescents Belonging to Sexual and Gender Minorities
Authors: Cardoso Bruno Luiz Avelino , Lima Ana Flávia Azevedo , Costa Fabiana Rachel Martins , Loose Christof , Liu Xi , Fabris Matteo Angelo
Published: July 2024

Culture is a central theme across various theories and disciplines, influencing behavior and self-perception through interactions within social groups, families, and legal systems. This influence extends to the general population and particularly impacts sexual and gender minorities (SGMs), resulting in minority stress that contributes to mental health issues and the development of Early Maladaptive Schemas (EMSs). Adolescents within these groups face typical developmental stressors—such as hormonal changes and societal pressures—compounded by prejudice, increasing their vulnerability to depression, anxiety, stress, substance abuse, and eating disorders. Despite these challenges, Schema Therapy (ST) lacks comprehensive studies on the sociocultural aspects influencing EMS acquisition in SGM adolescents. This theoretical review aims to fill this gap by exploring the impact of society and culture on EMS development within SGM adolescents. We recognize the broad spectrum of cultural influences and emphasize the importance of cultural sensitivity and diversity. This review specifically addresses how societal and cultural dynamics impact SGM individuals, acknowledging that while ethnic or other cultural factors are not the focus of this paper, they merit future research. This manuscript will discuss central topics and their impact on LGBTQIA+ youth, including (1) the background (definition of culture, lack of studies on ST focusing on culture, and studies on adverse psychological outcomes), (2) minority stress theory and prejudice against sexual and gender diversity (distal and proximal stressors and sociocultural aspects), (3) EMSs and unmet emotional needs, (4) ST affirmative strategies (working with schema modes, imagery rescripting, chair work, and photo techniques), and (5) final considerations (limitations and research agenda).
Crosstalk among Alternative Polyadenylation, Genetic Variants and Ubiquitin Modification Contribute to Lung Adenocarcinoma Risk
Authors: Wu Yutong , Yuan Yanqiong , Xu Huiwen , Zhang Wendi , Ning Anhui , Li Siqi , Chen Qiong , Tao Xiaobo , Pan Gongbu , Tian Tian , Zhang Lei , Chu Minjie , Cui Jiahua
Published: July 2024

Ubiquitin modification and alternative polyadenylation play crucial roles in the onset and progression of cancer. Hence, this study aims to comprehensively and deeply understand gene regulation and associated biological processes in lung adenocarcinoma (LUAD) by integrating both mechanisms. Alternative polyadenylation (APA)-related E3 ubiquitin ligases in LUAD were identified through multiple databases, and the association between selected genetic loci influencing gene expression (apaQTL-SNPs) and LUAD risk were evaluated through the GWAS database of the Female Lung Cancer Consortium in Asia (FLCCA). Subsequently, the interaction between RNF213 and ZBTB20, as well as their functional mechanisms in LUAD, were investigated using bioinformatics analysis, Western blot, co-immunoprecipitation, and colony formation experiments. A total of five apaQTL-SNPs (rs41301932, rs4494603, rs9890400, rs56066320, and rs41301932), located on RNF213, were significantly associated with LUAD risk (p < 0.05), and they inhibit tumor growth through ubiquitin-mediated degradation of ZBTB20.
Anakinra-Loaded Sphingomyelin Nanosystems Modulate In Vitro IL-1-Dependent Pro-Tumor Inflammation in Pancreatic Cancer
Authors: Abal-Sanisidro Marcelina , De Luca Michele , Roma Stefania , Ceraolo Maria Grazia , de la Fuente Maria , De Monte Lucia , Protti Maria Pia
Published: July 2024

Pancreatic cancer is a very aggressive disease with a dismal prognosis. The tumor microenvironment exerts immunosuppressive activities through the secretion of several cytokines, including interleukin (IL)-1. The IL-1/IL-1 receptor (IL-1R) axis is a key regulator in tumor-promoting T helper (Th)2- and Th17-type inflammation. Th2 cells are differentiated by dendritic cells endowed with Th2-polarizing capability by the thymic stromal lymphopoietin (TSLP) that is secreted by IL-1-activated cancer-associated fibroblasts (CAFs). Th17 cells are differentiated in the presence of IL-1 and other IL-1-regulated cytokines. In pancreatic cancer, the use of a recombinant IL-1R antagonist (IL1RA, anakinra, ANK) in in vitro and in vivo models has shown efficacy in targeting the IL-1/IL-1R pathway. In this study, we have developed sphingomyelin nanosystems (SNs) loaded with ANK (ANK-SNs) to compare their ability to inhibit Th2- and Th17-type inflammation with that of the free drug in vitro. We found that ANK-SNs inhibited TSLP and other pro-tumor cytokines released by CAFs at levels similar to ANK. Importantly, inhibition of IL-17 secretion by Th17 cells, but not of interferon-γ, was significantly higher, and at lower concentrations, with ANK-SNs compared to ANK. Collectively, the use of ANK-SNs might be beneficial in reducing the effective dose of the drug and its toxic effects.
Theranostics Using MCM-41-Based Mesoporous Silica Nanoparticles: Integrating Magnetic Resonance Imaging and Novel Chemotherapy for Breast Cancer Treatment
Authors: Pires Indira C. B. , Shuchi Samia I. , Tostes Braulio de V. A. , Santos Dayane K. D. do N. , Burnett William L. , Leonce Burke C. , Harvey Omar R. , Coffer Jeffery L. , de Sousa Filho Idio Alves , de Athayde-Filho Petrônio Filgueiras , Junior Severino A. , Mathis J. Michael
Published: July 2024

Advanced breast cancer remains a significant oncological challenge, requiring new approaches to improve clinical outcomes. This study investigated an innovative theranostic agent using the MCM-41-NH2-DTPA-Gd3⁺-MIH nanomaterial, which combined MRI imaging for detection and a novel chemotherapy agent (MIH 2.4Bl) for treatment. The nanomaterial was based on the mesoporous silica type, MCM-41, and was optimized for drug delivery via functionalization with amine groups and conjugation with DTPA and complexation with Gd3+. MRI sensitivity was enhanced by using gadolinium-based contrast agents, which are crucial in identifying early neoplastic lesions. MIH 2.4Bl, with its unique mesoionic structure, allows effective interactions with biomolecules that facilitate its intracellular antitumoral activity. Physicochemical characterization confirmed the nanomaterial synthesis and effective drug incorporation, with 15% of MIH 2.4Bl being adsorbed. Drug release assays indicated that approximately 50% was released within 8 h. MRI phantom studies demonstrated the superior imaging capability of the nanomaterial, with a relaxivity significantly higher than that of the commercial agent Magnevist. In vitro cellular cytotoxicity assays, the effectiveness of the nanomaterial in killing MDA-MB-231 breast cancer cells was demonstrated at an EC50 concentration of 12.6 mg/mL compared to an EC50 concentration of 68.9 mg/mL in normal human mammary epithelial cells (HMECs). In vivo, MRI evaluation in a 4T1 syngeneic mouse model confirmed its efficacy as a contrast agent. This study highlighted the theranostic capabilities of MCM-41-NH2-DTPA-Gd3⁺-MIH and its potential to enhance breast cancer management.
Epigenetic Regulation of the Renin–Angiotensin–Aldosterone System in Hypertension
Authors: Takeda Yoshimichi , Demura Masashi , Yoneda Takashi , Takeda Yoshiyu
Published: July 2024

Activation of the renin–angiotensin–aldosterone system (RAAS) plays an important pathophysiological role in hypertension. Increased mRNA levels of the angiotensinogen angiotensin-converting enzyme, angiotensin type 1 receptor gene, Agtr1a, and the aldosterone synthase gene, CYP11B2, have been reported in the heart, blood vessels, and kidneys in salt-sensitive hypertension. However, the mechanism of gene regulation in each component of the RAAS in cardiovascular and renal tissues is unclear. Epigenetic mechanisms, which are important for regulating gene expression, include DNA methylation, histone post-translational modifications, and microRNA (miRNA) regulation. A close association exists between low DNA methylation at CEBP-binding sites and increased AGT expression in visceral adipose tissue and the heart of salt-sensitive hypertensive rats. Several miRNAs influence AGT expression and are associated with cardiovascular diseases. Expression of both ACE and ACE2 genes is regulated by DNA methylation, histone modifications, and miRNAs. Expression of both angiotensinogen and CYP11B2 is reversibly regulated by epigenetic modifications and is related to salt-sensitive hypertension. The mineralocorticoid receptor (MR) exists in cardiovascular and renal tissues, in which many miRNAs influence expression and contribute to the pathogenesis of hypertension. Expression of the 11beta-hydroxysteroid dehydrogenase type 2 (HSD11B2) gene is also regulated by methylation and miRNAs. Epigenetic regulation of renal and vascular HSD11B2 is an important pathogenetic mechanism for salt-sensitive hypertension.
Apolipoproteine and KLOTHO Gene Variants Do Not Affect the Penetrance of Fragile X-Associated Tremor/Ataxia Syndrome
Authors: Winarni Tri Indah , Hwang Ye Hyun , Rivera Susan M. , Hessl David , Durbin-Johnson Blythe P. , Utari Agustini , Hagerman Randi , Tassone Flora
Published: July 2024

In this study, the potential role and interaction of the APOε and KLOTHO genes on the penetrance of fragile X-associated tremor/ataxia syndrome (FXTAS) and on the IQ trajectory were investigated. FXTAS was diagnosed based on molecular, clinical and radiological criteria. Males with the premutation (PM) over 50 years, 165 with and 34 without an FXTAS diagnosis, were included in this study and were compared based on their APO (ε2-ε3-ε4) and KLOTHO variant (KL-VS) genotypes. The effect of APOε4 on FXTAS stage and on diagnosis did not differ significantly by KL-VS genotype with interaction effect p = 0.662 and p = 0.91, respectively. In the FXTAS individuals with an APOε2 allele, a marginal significance was observed towards a larger decline in verbal IQ (VIQ) in individuals with an APOε4 allele compared to those without an APOε4 allele (p = 0.071). In conclusion, our findings suggest that the APOε4 and KL-VS genotypes alone or through their interaction effect do not appear to predispose to either FXTAS diagnosis or stage in male carriers of the PM allele. A further study is needed to establish the trend of IQ decline in the FXTAS individuals who carry APOε4 with APOε2 compared to those without APOε4.
Breaking the Chain: Protease Inhibitors as Game Changers in Respiratory Viruses Management
Authors: Papaneophytou Christos
Published: July 2024

Respiratory viral infections (VRTIs) rank among the leading causes of global morbidity and mortality, affecting millions of individuals each year across all age groups. These infections are caused by various pathogens, including rhinoviruses (RVs), adenoviruses (AdVs), and coronaviruses (CoVs), which are particularly prevalent during colder seasons. Although many VRTIs are self-limiting, their frequent recurrence and potential for severe health complications highlight the critical need for effective therapeutic strategies. Viral proteases are crucial for the maturation and replication of viruses, making them promising therapeutic targets. This review explores the pivotal role of viral proteases in the lifecycle of respiratory viruses and the development of protease inhibitors as a strategic response to these infections. Recent advances in antiviral therapy have highlighted the effectiveness of protease inhibitors in curtailing the spread and severity of viral diseases, especially during the ongoing COVID-19 pandemic. It also assesses the current efforts aimed at identifying and developing inhibitors targeting key proteases from major respiratory viruses, including human RVs, AdVs, and (severe acute respiratory syndrome coronavirus-2) SARS-CoV-2. Despite the recent identification of SARS-CoV-2, within the last five years, the scientific community has devoted considerable time and resources to investigate existing drugs and develop new inhibitors targeting the virus’s main protease. However, research efforts in identifying inhibitors of the proteases of RVs and AdVs are limited. Therefore, herein, it is proposed to utilize this knowledge to develop new inhibitors for the proteases of other viruses affecting the respiratory tract or to develop dual inhibitors. Finally, by detailing the mechanisms of action and therapeutic potentials of these inhibitors, this review aims to demonstrate their significant role in transforming the management of respiratory viral diseases and to offer insights into future research directions.
Alterations of the Fatty Acid Profile and the Expression of Genes Related to FA Metabolism in Cirrhotic Liver Tissue
Authors: Hliwa Aleksandra , Lange-Andrzejewska Oliwia , Laski Dariusz , Sledzinski Maciej , Remiszewski Piotr , Drobinska Anna , Mika Adriana , Sledzinski Tomasz
Published: July 2024

In addition to direct damage to hepatocytes, long-term ethanol consumption leads to lipid accumulation and hepatic steatosis, as well as to the dysregulation of lipid metabolism. The final step in various liver diseases is cirrhosis. The aim of this study was to compare the FA (fatty acids) profile and expression levels of genes involved in lipid metabolism in cirrhotic liver tissue and normal liver tissue. Exploring the changes in the FA profile and expression of genes related to fatty acid metabolism in cirrhotic liver tissue reveals a molecular landscape that goes beyond the surface of traditional liver function assessments. Understanding the shifts in gene expression and fatty acid composition in liver tissue opens avenues for interventions that may aid in the treatment of cirrhosis in the future.
Extra Virgin Olive Oil and Metabolic Diseases
Authors: Tsimihodimos Vasilis , Psoma Ourania
Published: July 2024

Over the last few decades, metabolic syndrome coexisting with cardiovascular disease has evolved into a pandemic, making the need for more food-oriented therapeutic approaches and a redefinition of lifestyle imperative, with the Mediterranean diet being the linchpin of this effort. Extra virgin olive oil (EVOO), the key pillar of the Mediterranean diet and one of the most notorious edible oils worldwide, owes its popularity not only to its characteristic aromas and taste but mainly to a series of beneficial health attributes including anti-diabetic, hypolipidemic, anti-hypertensive and anti-obesity actions. In this narrative review, we aimed to illustrate and enlighten EVOO’s metabolic properties through a pathogenetic approach, investigating its potential role in metabolic and cardiovascular health.