bluuebunny/crossref_metadata_2025 huggingface dataset:
CFD Model of Refuelling through the Entire HRS Equipment: The Start-Up Phase Simulations
Authors: Molkov Vladimir , Ebne-Abbasi Hazhir , Makarov Dmitriy
Published: September 2023

Refuelling hydrogen-powered cars, buses, trucks, trains, ships, and planes is a technological challenge. The absence of contemporary CFD models of refuelling through the entire hydrogen refuelling station (HRS) equipment is one of the scientific bottlenecks. Detailed refuelling protocols for more than 10 kg of hydrogen, e.g., for heavy-duty vehicles, are absent. A thoroughly validated CFD model for simulations of the refuelling process through the entire equipment of the HRS is needed for protocols’ development. This study aims to numerically simulate the start-up phase of the refuelling procedure at HRS using the developed CFD model. The simulations through the entire HRS equipment are compared against unique experimental data of NREL and demonstrated agreement with measured pressure and temperature dynamics in onboard storage tanks during the start-up phase while having less than 5% deviation. The CFD model demonstrates excellent predictive capability and is time efficient. The simulation time of the start-up phase of 14 s duration is about 2 h on a 32-core CPU.
Tooth Wear and Periodontal Status in a Cluster of Middle-Aged Adults in Northern Greece
Authors: Theodoridis Charis , Menexes George , Topitsoglou Vasiliki , Kalfas Sotirios
Published: September 2023

The present observational study aims to investigate the prevalence of tooth wear (TW) in a cluster of Northern Greek adults aged 35–44 years and, additionally, to assess any probable associations and interrelationships between TW and periodontal condition and sociodemographic factors. A cross-sectional design was embraced identically to our previous study on senior citizens. A sample of 531 individuals was considered, and they were examined according to WHO guidelines for national pathfinder surveys by three calibrated dentists-examiners in different urban and rural areas of Northern Greece. Simplified Tooth Wear Index (TWI), Community Periodontal Index (CPI), Attachment Loss (AL) and simplified Oral Hygiene Index (OHI-S) were screened and calculated in a patient-level approach. TW is quite prevalent among middle-aged adults in Northern Greece, although it remains lower compared to older age groups. All the aforementioned periodontal indices were correlated significantly with TW; moreover, a tendency was detected for there to be more tooth wear in older age participants within the specific age group, in men as compared with women, in persons with lower educational levels and in those residing in rural areas.
Learning to Live with HIV: The Experience of a Group of Young Chilean Men
Authors: Calderón Silva Macarena Belén , Ferrer Lagunas Lilian Marcela , Cianelli Rosina
Published: September 2023

Young men aged 20–29 present the highest rates of HIV in Chile, yet little is known about their experiences after diagnosis. This study sheds light on the meaning of living with HIV for young gay Chilean males. Qualitative analysis of 11 in-depth interviews, employing Heidegger’s hermeneutical approach, resulted in a depiction of feelings of loneliness and sadness, encounters with discrimination, and a lack of social support. Participants also offer recommendations on utilizing peer support as an essential component for programs targeting young gay men living with HIV in the capital of Chile. Additionally, they suggest that health care providers play a crucial role in supporting the process of accepting the condition. Conclusions: It is imperative to enhance psychological support by integrating it into the HIV program for all clients and families facing crisis situations. The establishment of support groups within hospitals that are part of the national program providers is recommended. Education on comprehensive sexuality should be provided in educational institutions, alongside visible campaigns across all media platforms to dismantle the stigma linked to the disease. Lastly, improvements in care duration and support from health services can be achieved through the provision of comprehensive care founded on unconditional acceptance.
Features of Congenital Arthrogryposis Due to Abnormalities in Collagen Homeostasis, a Scoping Review
Authors: Picker Sarah MacKenzie , Parker George , Gissen Paul
Published: September 2023

Congenital arthrogryposis (CA) refers to the presence of multiple contractures at birth. It is a feature of several inherited syndromes, notable amongst them are disorders of collagen formation. This review aims to characterize disorders that directly or indirectly impact collagen structure and function leading to CA in search for common phenotypic or pathophysiological features, possible genotype–phenotype correlation, and potential novel treatment approaches based on a better understanding of the underlying pathomechanism. Nine genes, corresponding to five clinical phenotypes, were identified after a literature search. The most notable trend was the extreme phenotype variability. Clinical features across all syndromes ranged from subtle with minimal congenital contractures, to severe with multiple congenital contractures and extra-articular features including skin, respiratory, or other manifestations. Five of the identified genes were involved in the function of the Lysyl Hydroxylase 2 or 3 enzymes, which enable the hydroxylation and/or glycosylation of lysyl residues to allow the formation of the collagen superstructure. Whilst current treatment approaches are post-natal surgical correction, there are also potential in-utero therapies being developed. Cyclosporin A showed promise in treating collagen VI disorders although there is an associated risk of immunosuppression. The treatments that could be in the clinical trials soon are the splice correction therapies in collagen VI-related disorders.
Metallic Nanowires Self-Assembled in Quasi-Circular Nanomolds Templated by DNA Origami
Authors: Ruiz Arce David Daniel , Jazavandi Ghamsari Shima , Erbe Artur , Samano Enrique C.
Published: September 2023

The self-assembly of conducting nanostructures is currently being investigated intensively in order to evaluate the feasibility of creating novel nanoelectronic devices and circuits using such pathways. In particular, methods based on so-called DNA Origami nanostructures have shown great potential in the formation of metallic nanowires. The main challenge of this method is the reproducible generation of very well-connected metallic nanostructures, which may be used as interconnects in future devices. Here, we use a novel design of nanowires with a quasi-circular cross-section as opposed to rectangular or uncontrolled cross-sections in earlier studies. We find indications that the reliability of the fabrication scheme is enhanced and the overall resistance of the wires is comparable to metallic nanostructures generated by electrochemistry or top-down methods. In addition, we observe that some of the nanowires are annealed when passing a current through them, which leads to a clear enhancement for the conductance. We envision that these nanowires provide further steps towards the successful generation of nanoelectronics using self-assembly.
Mechanism of Action and Efficiency of Ag3PO4-Based Photocatalysts for the Control of Hazardous Gram-Positive Pathogens
Authors: Paluch Emil , Seniuk Alicja , Plesh Gustav , Widelski Jarosław , Szymański Damian , Wiglusz Rafał J. , Motola Martin , Dworniczek Ewa
Published: September 2023

Silver phosphate and its composites have been attracting extensive interest as photocatalysts potentially effective against pathogenic microorganisms. The purpose of the present study was to investigate the mechanism of bactericidal action on cells of opportunistic pathogens. The Ag3PO4/P25 (AGP/P25) and Ag3PO4/HA (HA/AGP) powders were prepared via a co-precipitation method. Thereafter, their antimicrobial properties against Enterococcus faecalis, Staphylococcus epidermidis, and Staphylococcus aureus (clinical and reference strains) were analyzed in the dark and after exposure to visible light (VIS). The mechanism leading to cell death was investigated by the leakage of metabolites and potassium ions, oxidative stress, and ROS production. Morphological changes of the bacterial cells were visualized by transmission electron microscopy (TEM) and scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy (SEM EDS) analysis. It has been shown that Ag3PO4-based composites are highly effective agents that can eradicate 100% of bacterial populations during the 60 min photocatalytic inactivation. Their action is mainly due to the production of hydroxyl radicals and photogenerated holes which lead to oxidative stress in cells. The strong affinity to the bacterial cell wall, as well as the well-known biocidal properties of silver itself, increase undoubtedly the antimicrobial potential of the Ag3PO4-based composites.
Changes in Hemoglobin Properties in Complex with Glutathione and after Glutathionylation
Authors: Kuleshova Iuliia D. , Zaripov Pavel I. , Poluektov Yuri M. , Anashkina Anastasia A. , Kaluzhny Dmitry N. , Parshina Evgeniia Yu. , Maksimov Georgy V. , Mitkevich Vladimir A. , Makarov Alexander A. , Petrushanko Irina Yu.
Published: September 2023

Hemoglobin is the main protein of red blood cells that provides oxygen transport to all cells of the human body. The ability of hemoglobin to bind the main low-molecular-weight thiol of the cell glutathione, both covalently and noncovalently, is not only an important part of the antioxidant protection of red blood cells, but also affects its affinity for oxygen in both cases. In this study, the properties of oxyhemoglobin in complex with reduced glutathione (GSH) and properties of glutathionylated hemoglobin bound to glutathione via an SS bond were characterized. For this purpose, the methods of circular dichroism, Raman spectroscopy, infrared spectroscopy, tryptophan fluorescence, differential scanning fluorimetry, and molecular modeling were used. It was found that the glutathionylation of oxyhemoglobin caused changes in the secondary structure of the protein, reducing the alpha helicity, but did not affect the heme environment, tryptophan fluorescence, and the thermostability of the protein. In the noncovalent complex of oxyhemoglobin with reduced glutathione, the secondary structure of hemoglobin remained almost unchanged; however, changes in the heme environment and the microenvironment of tryptophans, as well as a decrease in the protein’s thermal stability, were observed. Thus, the formation of a noncovalent complex of hemoglobin with glutathione makes a more significant effect on the tertiary and quaternary structure of hemoglobin than glutathionylation, which mainly affects the secondary structure of the protein. The obtained data are important for understanding the functioning of glutathionylated hemoglobin, which is a marker of oxidative stress, and hemoglobin in complex with GSH, which appears to deposit GSH and release it during deoxygenation to increase the antioxidant protection of cells.
The Therapeutic Potential of CDK4/6 Inhibitors, Novel Cancer Drugs, in Kidney Diseases
Authors: Liang Xuan-Bing , Dai Zhi-Cheng , Zou Rong , Tang Ji-Xin , Yao Cui-Wei
Published: September 2023

Inflammation is a crucial pathological feature in cancers and kidney diseases, playing a significant role in disease progression. Cyclin-dependent kinases CDK4 and CDK6 not only contribute to cell cycle progression but also participate in cell metabolism, immunogenicity and anti-tumor immune responses. Recently, CDK4/6 inhibitors have gained approval for investigational treatment of breast cancer and various other tumors. Kidney diseases and cancers commonly exhibit characteristic pathological features, such as the involvement of inflammatory cells and persistent chronic inflammation. Remarkably, CDK4/6 inhibitors have demonstrated impressive efficacy in treating non-cancerous conditions, including certain kidney diseases. Current studies have identified the renoprotective effect of CDK4/6 inhibitors, presenting a novel idea and potential direction for treating kidney diseases in the future. In this review, we briefly reviewed the cell cycle in mammals and the role of CDK4/6 in regulating it. We then provided an introduction to CDK4/6 inhibitors and their use in cancer treatment. Additionally, we emphasized the importance of these inhibitors in the treatment of kidney diseases. Collectively, growing evidence demonstrates that targeting CDK4 and CDK6 through CDK4/6 inhibitors might have therapeutic benefits in various cancers and kidney diseases and should be further explored in the future.
17 β-Estradiol Impedes Aortic Root Dilation and Rupture in Male Marfan Mice
Authors: Saddic Louis , Escopete Sean , Zilberberg Lior , Kalsow Shannon , Gupta Divya , Eghbali Mansoureh , Parker Sarah
Published: September 2023

Marfan syndrome causes a hereditary form of thoracic aortic aneurysms with worse outcomes in male compared to female patients. In this study, we examine the effects of 17 β-estradiol on aortic dilation and rupture in a Marfan mouse model. Marfan male mice were administered 17 β-estradiol, and the growth in the aortic root, along with the risk of aortic rupture, was measured. Transcriptomic profiling was used to identify enriched pathways from 17 β-estradiol treatments. Aortic smooth muscle cells were then treated with cytokines to validate functional mechanisms. We show that 17 β-estradiol decreased the size and rate of aortic root dilation and improved survival from rupture. The Marfan transcriptome was enriched in inflammatory genes, and the addition of 17 β-estradiol modulated a set of genes that function through TNFα mediated NF-κB signaling. In addition, 17 β-estradiol suppressed the induction of these TNFα induced genes in aortic smooth muscle cells in vitro in an NF-κB dependent manner, and 17 β-estradiol decreased the formation of adventitial inflammatory foci in aortic roots in vivo. In conclusion, 17 β-estradiol protects against the dilation and rupture of aortic roots in Marfan male mice through the inhibition of TNFα-NF-κB signaling.
Acrolein Induces Retinal Abnormalities of Alzheimer’s Disease in Mice
Authors: Wang Shuyi , Jiang Xiuying , Peng Weijia , Yang Shuangjian , Pi Rongbiao , Zhou Shiyou
Published: September 2023

It is reported that retinal abnormities are related to Alzheimer’s disease (AD) in patients and animal models. However, it is unclear whether the retinal abnormities appear in the mouse model of sporadic Alzheimer’s disease (sAD) induced by acrolein. We investigated the alterations of retinal function and structure, the levels of β-amyloid (Aβ) and phosphorylated Tau (p-Tau) in the retina, and the changes in the retinal vascular system in this mouse model. We demonstrated that the levels of Aβ and p-Tau were increased in the retinas of mice from the acrolein groups. Subsequently, a decreased amplitudes of b-waves in the scotopic and photopic electroretinogram (ERG), decreased thicknesses of the retinal nerve fiber layer (RNFL) in the retina, and slight retinal venous beading were found in the mice induced by acrolein. We propose that sAD mice induced by acrolein showed abnormalities in the retina, which may provide a valuable reference for the study of the retina in sAD.
Closer to the Reality—Proteome Changes Evoked by Endometrial Scratching in Fertile Females
Authors: Scheliga Iwona , Baston-Buest Dunja M. , Poschmann Gereon , Stuehler Kai , Kruessel Jan-Steffen , Bielfeld Alexandra P.
Published: September 2023

Endometrial scratching (ES) has been widely used in assisted reproductive technology to possibly improve pregnancy rates, but its exact mechanism is still not understood or investigated, and its benefits are controversially discussed. Hypothetically, ES may trigger a local immune response, leading to an improved endometrial receptivity. So far, it has been shown that ES affects the gene expression of cytokines, growth factors, and adhesive proteins, potentially modulating inflammatory pathways and adhesion molecule expression. Our pilot study applying proteomic analysis reveals that ES probably has an impact on the proteins involved in immune response pathways and cytoskeleton formation, which could potentially increase endometrial receptivity. Specifically, proteins that are involved in the immune response and cytoskeleton regulation showed a trend toward higher abundance after the first ES. On the other hand, proteins with a decreasing abundance after the first ES play roles in the regulation of the actin cytoskeleton and cellular processes such as intracellular transport, apoptosis, and autophagy. These trends in protein changes suggest that ES may affect endometrial tissue stiffness and extracellular matrix remodeling, potentially enhancing the embryos’ implantation. To our knowledge, this pilot study provides, for the first time, data investigating potential changes in the endometrium due to the scratching procedure that might explain its possible benefit for patients in infertility treatment. Furthermore, the proteome of a group of patients suffering from repeated implantation failure was compared to that of the fertile group in order to transfer the basic science to clinical routine and application.
Downregulation of Zebrafish Cytosolic Sialidase Neu3.2 Affects Skeletal Muscle Development
Authors: Zizioli Daniela , Codenotti Silvia , Benaglia Giuliana , Manzoni Marta , Massardi Elena , Fanzani Alessandro , Borsani Giuseppe , Monti Eugenio
Published: September 2023

Sialidases remove terminal sialic acids residues from the non-reducing ends of glycoconjugates. They have been recognized as catabolic enzymes that work within different subcellular compartments and can ensure the proper turn-over of glycoconjugates. Four mammalian sialidases (NEU1-4) exist, with different subcellular localization, pH optimum and substrate specificity. In zebrafish, seven different sialidases, with high homology to mammalian counterparts, have been identified. Zebrafish Neu3.2 is similar to the human cytosolic sialidase NEU2, which is involved in skeletal muscle differentiation and exhibits a broad substrate specificity toward gangliosides and glycoproteins. In zebrafish neu3.2, mRNA is expressed during somite development, and its enzymatic activity has been detected in the skeletal muscle and heart of adult animals. In this paper, 1–4-cell-stage embryos injected with neu3.2 splice-blocking morpholino showed severe embryonic defects, mainly in somites, heart and anterior–posterior axis formation. Myog and myod1 expressions were altered in morphants, and impaired musculature formation was associated with a defective locomotor behavior. Finally, the co-injection of Neu2 mouse mRNA in morphants rescued the phenotype. These data are consistent with the involvement of cytosolic sialidase in pathologies related to muscle formation and support the validity of the model to investigate the pathogenesis of the diseases.
Peri-Operative Kinetics of Plasma Mitochondrial DNA Levels during Living Donor Kidney Transplantation
Authors: Kroneisl Marie , Spraakman Nora , Koomen Jeroen , Hijazi Zeinab , Hoogstra-Berends Femke , Leuvenink Henri , Struys Michel , Henning Rob , Nieuwenhuijs-Moeke Gertrude
Published: September 2023

During ischemia and reperfusion injury (IRI), mitochondria may release mitochondrial DNA (mtDNA). mtDNA can serve as a propagator of further injury but in specific settings has anti-inflammatory capacities as well. Therefore, the aim of this study was to study the perioperative dynamics of plasma mtDNA during living donor kidney transplantation (LDKT) and its potential as a marker of graft outcome. Fifty-six donor–recipient couples from the Volatile Anesthetic Protection of Renal Transplants-1 (VAPOR-1) trial were included. Systemic venous, systemic arterial, and renal venous samples were taken at multiple timepoints during and after LDKT. Levels of mtDNA genes changed over time and between vascular compartments. Several donor, recipient, and transplantation-related variables significantly explained the course of mtDNA genes over time. mtDNA genes predicted 1-month and 24-month estimated glomerular filtration rate (eGFR) and acute rejection episodes in the two-year follow-up period. To conclude, mtDNA is released in plasma during the process of LDKT, either from the kidney or from the whole body in response to transplantation. While circulating mtDNA levels positively and negatively predict post-transplantation outcomes, the exact mechanisms and difference between mtDNA genes are not yet understood and need further exploration.
GSCEU-Net: An End-to-End Lightweight Skin Lesion Segmentation Model with Feature Fusion Based on U-Net Enhancements
Authors: Hao Shengnan , Wu Haotian , Jiang Yanyan , Ji Zhanlin , Zhao Li , Liu Linyun , Ganchev Ivan
Published: September 2023

Accurate segmentation of lesions can provide strong evidence for early skin cancer diagnosis by doctors, enabling timely treatment of patients and effectively reducing cancer mortality rates. In recent years, some deep learning models have utilized complex modules to improve their performance for skin disease image segmentation. However, limited computational resources have hindered their practical application in clinical environments. To address this challenge, this paper proposes a lightweight model, named GSCEU-Net, which is able to achieve superior skin lesion segmentation performance at a lower cost. GSCEU-Net is based on the U-Net architecture with additional enhancements. Firstly, the partial convolution (PConv) module, proposed by the FasterNet model, is modified to an SConv module, which enables channel segmentation paths of different scales. Secondly, a newly designed Ghost SConv (GSC) module is proposed for incorporation into the model’s backbone, where the Separate Convolution (SConv) module is aided by a Multi-Layer Perceptron (MLP) and the output path residuals from the Ghost module. Finally, the Efficient Channel Attention (ECA) mechanism is incorporated at different levels into the decoding part of the model. The segmentation performance of the proposed model is evaluated on two public datasets (ISIC2018 and PH2) and a private dataset. Compared to U-Net, the proposed model achieves an IoU improvement of 0.0261 points and a DSC improvement of 0.0164 points, while reducing the parameter count by 190 times and the computational complexity by 170 times. Compared to other existing segmentation models, the proposed GSCEU-Net model also demonstrates superiority, along with an advanced balance between the number of parameters, complexity, and segmentation performance.
Diet, Oral Hygiene Habits, and Approach to Dental Visits of Early School-Aged Children during the COVID-19 Pandemic and Possible Long-Term Health Consequences
Authors: Torlińska-Walkowiak Natalia , Łukaszewicz Karolina , Morawska Alicja , Sowińska Anna , Pawlaczyk-Kamieńska Tamara , Opydo-Szymaczek Justyna
Published: September 2023

Introduction: Early school-aged children are in a transitional phase from primary to permanent dentition. Established dietary and oral hygiene habits will influence the condition of the oral cavity in the future. Aim: This study aimed to evaluate alterations in early school children’s dietary and oral hygiene practices during the COVID-19 pandemic and to anticipate potential long-term health implications. Material and Methods: This cross-sectional online study involved guardians of Polish children aged 6–10 years, living in Western Poland, who were socially isolated at home during the COVID-19 pandemic. A total of 180 guardians were invited to participate in this study. The questionnaire included 17 questions divided into four different sections—the child’s anthropometric data, dietary habits, oral health, and attitude to dental visits. Results: The survey was completed by 106 guardians. The mean (standard deviation) age of the children was 8.12 (0.93) years (range 6–10 years). Overall, 24.5% of the surveyed individuals reported buying healthy products (fresh vegetables, fruit) more frequently during the pandemic. Furthermore, 35.8% admitted to snacking between meals more frequently and 16.0% less frequently. Almost one-quarter of the parents acknowledged that their children were less motivated to maintain oral hygiene during the pandemic and 28.3% declared a lower frequency of visits to the dentist from the outbreak of the pandemic for two main reasons: a fear of coronavirus transmission and economic reasons. Conclusions: In the group studied, the results of the assessment indicate that the COVID-19 pandemic had some effects on oral health that may lead to an increased risk of oral disease development, such as tooth decay in children. None of the respondents noticed an increase in their child’s motivation about good oral hygiene despite spending more time at home. The irregularity of follow-up visits for one-third of the respondents hindered preventive measures and the continuation of dental treatment.
Hybrid Treatment of Complex Diseases of the Aortic Arch and Descending Thoracic Aorta by Frozen Elephant Trunk Technique
Authors: Porterie Jean , Hostalrich Aurélien , Dagenais François , Marcheix Bertrand , Chaufour Xavier , Ricco Jean-Baptiste
Published: September 2023

The surgical management of acute and chronic complex diseases involving the aortic arch and the descending thoracic aorta remains challenging. Hybrid procedures associating total open arch replacement and stent-grafting of the proximal descending aorta were developed to allow a potential single-stage treatment, promote remodeling of the downstream aorta, and facilitate a potential second-stage thoracic endovascular aortic repair by providing an ideal landing zone. While these approaches initially used various homemade combinations of available conventional prostheses and stent-grafts, the so-called frozen elephant trunk technique emerged with the development of several custom-made hybrid prostheses. The aim of this study was to review the contemporary outcomes of this technique in the management of complex aortic diseases, with a special focus on procedural planning, organ protection and monitoring, refinements in surgical techniques, and long-term follow-up.
Primary Hemostasis Disorders as a Cause of Heavy Menstrual Bleeding in Women of Reproductive Age
Authors: Kontogiannis Athanasios , Matsas Alkis , Valsami Serena , Livanou Maria Effrosyni , Panoskaltsis Theodoros , Christopoulos Panagiotis
Published: September 2023

Heavy menstrual bleeding (HMB) is a common clinical condition affecting adolescent and adult women and compromising their quality of life. Primary hemostasis disorders, affecting platelet plug formation, can be the underlying cause of HMB. They comprise a heterogeneous group of diseases with Von Willebrand disease (VWD) being the most commonly diagnosed; other disorders in this group that have been linked to HMB include (a) Glanzmann thrombasthenia, (b) Bernard–Soulier syndrome, (c) Hermansky–Pudlak syndrome, (d) immune thrombocytopenia (ITP), and (e) Ehlers–Danlos syndromes (EDS) and hypermobility spectrum disorders (HSD). Diagnosing these diseases can be challenging, as the basic laboratory investigations can be within the normal range. Thus, identification of specific clinical features and a thorough hematologic workup can be very important, providing the correct diagnosis. Proper diagnosis of the underlying disorder is important, as management may vary accordingly. Although disease-specific management guidelines exist for some of these disorders such as VWD and ITP, due to the rarity of most primary hemostasis disorders, the best approach for the management of HMB in these women remains elusive. The goal of this study was to create an informative, comprehensive review of the primary hemostasis disorders that have been linked to HMB. This study provides a summary of the basic published information regarding epidemiology, pathophysiology, clinical phenotype, diagnosis, and treatment of HMB in those diseases and serves as a reference guide for further reading.
Epidemiology, Diagnosis, Treatment, and Prognosis of Infective Endocarditis
Authors: Martínez-Sellés Manuel , Muñoz Patricia
Published: September 2023

Infective endocarditis (IE) has experienced enormous changes in recent decades [...]
Three Ablation Techniques for Atrial Fibrillation during Concomitant Cardiac Surgery: A Systematic Review and Network Meta-Analysis
Authors: Hanafy Dudy Arman , Erdianto Wahyu Prima , Husen Theresia Feline , Nathania Ilona , Vidya Ananda Pipphali , Angelica Ruth , Suwatri Widya Trianita , Lintangella Pasati , Prasetyo Priscillia , Sugisman
Published: September 2023

Atrial fibrillation (AF) ablation is a frequent procedure used in concomitant cardiac surgery. However, uncertainty still exists concerning the optimal extent of lesion sets. Hence, the objective of this study was to assess the results of various ablation techniques, aiming to offer a reference for clinical decision making. This review is listed in the prospective register of systematic reviews (PROSPERO) under ID CRD42023412785. A comprehensive search was conducted across eight databases (Scopus, Google Scholar, EBSCOHost, PubMed, Medline, Wiley, ProQuest, and Embase) up to 18 April 2023. Studies were critically appraised using the Cochrane Risk of Bias 2.0 for randomized control trials (RCTs) and the Newcastle Ottawa Scale adapted by the Agency for Healthcare Research and Quality (AHRQ) for cohort studies. Forest plots of pooled effect estimates and surface under the cumulative ranking (SUCRA) were used for the analysis. Our analysis included 39 studies and a total of 7207 patients. Both bi-atrial ablation (BAA) and left atrial ablation (LAA) showed similar efficacy in restoring sinus rhythm (SR; BAA (77.9%) > LAA (76.2%) > pulmonary vein isolation (PVI; 66.5%); LAA: OR = 1.08 (CI 0.94–1.23); PVI: OR = 1.36 (CI 1.08–1.70)). However, BAA had higher pacemaker implantation (LAA: OR = 0.51 (CI 0.37–0.71); PVI: OR = 0.52 (CI 0.31–0.86)) and reoperation rates (LAA: OR = 0.71 (CI 0.28–1.45); PVI: OR = 0.31 (CI 0.1–0.64)). PVI had the lowest efficacy in restoring SR and a similar complication rate to LAA, but had the shortest procedure time (Cross-clamp (Xc): PVI (93.38) > LAA (37.36) > BAA (13.89)); Cardiopulmonary bypass (CPB): PVI (93.93) > LAA (56.04) > BAA (0.03)). We suggest that LAA is the best surgical technique for AF ablation due to its comparable effectiveness in restoring SR, its lower rate of pacemaker requirement, and its lower reoperation rate compared to BAA. Furthermore, LAA ranks as the second-fastest procedure after PVI, with a similar CPB time.
Medium-Temperature Glass-Composite Phosphate Materials for the Immobilization of Chloride Radioactive Waste
Authors: Frolova Anna V. , Belova Ksenia Y. , Vinokurov Sergey E.
Published: September 2023

Among the many radiochemical problems, the search for new materials and technologies for the immobilization of radioactive waste remains relevant, and the range continues to change and expand. The possibility of immobilizing the spent chloride electrolyte after the pyrochemical processing of the mixed uranium-plutonium spent nuclear fuel of the new fast reactor BREST-OD-300 on lead coolant into glass-composite phosphate materials synthesized at temperatures of 650–750 °C was studied. The structure of the obtained samples was studied using XRD and SEM/EDS methods. It has been shown that the spent electrolyte simulator components create stable mixed pyrophosphate phases in the glass composite structure. The materials were found to have high hydrolytic stability. This indicates the promise of using phosphate glass composites as materials for the reliable immobilization of the spent electrolyte.
Divergent Responses of Hydrophilic CdSe and CdSe@CdS Core–Shell Nanocrystals in Apoptosis and In Vitro Cancer Cell Imaging: A Comparative Analysis
Authors: Das Kishan , Bhatt Neelima , Parambil Ajith Manayil , Kumari Kajal , Kumar Raj , Rawat Kamla , Rajamani Paulraj , Bohidar Himadri B. , Nadeem Ahmed , Muthupandian Saravanan , Meena Ramovatar
Published: September 2023

With their distinctive core–shell design, core–shell nanocrystals have drawn interest in catalysis, medicinal research, and nanotechnology. These nanocrystals have a variety of characteristics and possible uses. The application of core–shell nanocrystals offers significant potential in increasing diagnostic and therapeutic approaches for cancer research in apoptosis and in vitro cancer cell imaging. In the present study, we investigated the fluorescence behavior of hydrophilic CdSe (core-only) and CdSe@CdS (core–shell) nanocrystals (NCs) and their potential in cancer cell imaging. The addition of a CdS coating to CdSe NCs increased the fluorescence intensity tenfold. The successful fabrication of core–shell CdSe@CdS nanocrystals was proven by a larger particle size (evaluated via DLS and TEM) and their XRD pattern and surface morphology compared to CdSe (core-only) NCs. When these NCs were used for bioimaging in MCF-7 and HEK-293 cell lines, they demonstrated excellent cellular uptake due to higher fluorescence intensity within cancerous cells than normal cells. Comparative cytotoxicity studies revealed that CdSe NCs were more toxic to all three cell lines (HEK-293, MCF-7, and HeLa) than CdSe@CdS core–shell structures. Furthermore, a decrease in mitochondrial membrane potential and intracellular ROS production supported NCs inducing oxidative stress, which led to apoptosis via the mitochondria-mediated pathway. Increased cytochrome c levels, regulation of pro-apoptotic gene expression (e.g., p53, Bax), and down-regulation of Bcl-2 all suggested cellular apoptosis occurred via the intrinsic pathway. Significantly, at an equivalent dose of core–shell NCs, core-only NCs induced more oxidative stress, resulting in increased apoptosis. These findings shed light on the role of a CdS surface coating in reducing free radical release, decreasing cytotoxicity, and improving fluorescence, advancing the field of cell imaging.
Marine Collision Avoidance Route Planning Model for MASS Based on Domain-Based Predicted Area of Danger
Authors: Lu Chao-Wei , Hsueh Chao-Kuang , Chuang Yung-Lin , Lai Ching-Ming , Yang Fuh-Shyong
Published: September 2023

When the own ship encounters target ships in a close-quarter situation, an officer on watch needs to safely and timely alter the course of the vessel to avoid a collision. If ECDIS can automatically collect the navigation parameters and plot areas of collision as quasi-static obstruction areas, it will be much easier for seafarers to implement effective route planning. Hence, this study focuses on developing the MCARP model as a theoretical concept based on the DPAD model and LCD model. By operating the MCARP using ArcGIS, DPADs and several effective collision avoidance routes can be generated and imported into ECDIS based on AIS information at large scales and ample time. The graphic overlay of DPADs and effective routes on ECDIS can serve as a collision avoidance strategy reference for the personnel controlling maritime autonomous surface ships. Finally, different ships encountering situations were input into a Transas navigational simulator. The simulation results showed that own ship could avoid collisions with multiple target ships at distances larger than the preset collision avoidance distances, which also indicated that MCARP is practically feasible.
A Numerical Study on the Hydrodynamic Performance of a Tanker in Bow Sea Conditions Depending on Restraint Conditions
Authors: Lee Soon-Hyun , Hwang Seunghyun , Kim Hwi-Su , Hyun Yeo-Jin , Lee Sun-Kyu , Paik Kwang-Jun
Published: September 2023

The importance of accurate ship performance estimation is increasing for efficient ship operation. Ship performance has been evaluated through model tests in the past, but there are limitations in terms of facilities and costs. With the spread of high-performance computers, the method of evaluating the performance of a ship by numerical analysis, especially computational fluid dynamics (CFD), has become common. There have been many numerical studies on added resistance under various wave conditions for many years, showing a high reliability. Meanwhile, most of the studies were conducted under conditions where the degree of freedom (DOF) of the ship was limited due to computational complexity. In this study, we tried to compare the added resistance performance and fluid dynamics of S-VLCC with 6 DOFs in the regular wave conditions. One of the methods for utilizing the 6 DOFs is the soft-mooring system, which allows springs to be attached to the bow and stern to recover the non-restoring force of the hull. The second method considers the free-running condition. The virtual disk is used for the self-propulsion of the ship, and the rudder can be rotated to maintain its course. The propeller rotation speed and rudder angle are controlled through PID control. The bow wave (ψ = 180°) and oblique wave (ψ = 150°, 120°) conditions were considered, and various regular wave conditions from short to long wavelengths were regarded. The effects of restraint conditions on the added resistance and motion response amplitude operator (RAO), according to each wave condition, were compared. As a result, there was a difference in the roll motion for each restraint condition, and the y-direction force and yaw moment generated on the hull were compared to analyze the cause. In addition, we observed the change in flow characteristics by comparing the streamlines around the hull and the nominal wake on the propeller plane.
Axenic Long-Term Cultivation of Pneumocystis jirovecii
Authors: Riebold Diana , Mahnkopf Marie , Wicht Kristina , Zubiria-Barrera Cristina , Heise Jan , Frank Marcus , Misch Daniel , Bauer Torsten , Stocker Hartmut , Slevogt Hortense
Published: September 2023

Pneumocystis jirovecii, a fungus causing severe Pneumocystis pneumonia (PCP) in humans, has long been described as non-culturable. Only isolated short-term experiments with P. jirovecii and a small number of experiments involving animal-derived Pneumocystis species have been published to date. However, P. jirovecii culture conditions may differ significantly from those of animal-derived Pneumocystis, as there are major genotypic and phenotypic differences between them. Establishing a well-performing P. jirovecii cultivation is crucial to understanding PCP and its pathophysiological processes. The aim of this study, therefore, was to develop an axenic culture for Pneumocystis jirovecii. To identify promising approaches for cultivation, a literature survey encompassing animal-derived Pneumocystis cultures was carried out. The variables identified, such as incubation time, pH value, vitamins, amino acids, and other components, were trialed and adjusted to find the optimum conditions for P. jirovecii culture. This allowed us to develop a medium that produced a 42.6-fold increase in P. jirovecii qPCR copy numbers after a 48-day culture. Growth was confirmed microscopically by the increasing number and size of actively growing Pneumocystis clusters in the final medium, DMEM-O3. P. jirovecii doubling time was 8.9 days (range 6.9 to 13.6 days). In conclusion, we successfully cultivated P. jirovecii under optimized cell-free conditions in a 70-day long-term culture for the first time. However, further optimization of the culture conditions for this slow grower is indispensable.
Pharmacogenetic Variants Associated with Fluoxetine Pharmacokinetics from a Bioequivalence Study in Healthy Subjects
Authors: Díaz-Tufinio Carlos Alejandro , Palma-Aguirre José Antonio , Gonzalez-Covarrubias Vanessa
Published: September 2023

Fluoxetine is one of the most prescribed antidepressants, yet it still faces challenges due to high intersubject variability in patient response. Mainly metabolized by the highly polymorphic gene CYP2D6, important differences in plasma concentrations after the same doses are found among individuals. This study investigated the association of fluoxetine pharmacokinetics (PK) with pharmacogenetic variants. A bioequivalence crossover trial (two sequences, two periods) was conducted with fluoxetine 20 mg capsules, in 24 healthy subjects. Blood samples for fluoxetine determination were collected up to 72 h post-dose. Subjects were genotyped and single nucleotide variants (SNV) were selected using a candidate gene approach, and then associated with the PK parameters. Bioequivalence was confirmed for the test formulation. We found 34 SNV on 10 genes with a quantifiable impact on the PK of fluoxetine in the randomized controlled trial. Out of those, 29 SNVs belong to 7 CYPs (CYP1A2, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP3A4, CYP3A5), and 5 SNVs to 3 genes impacting the pharmacodynamics and efficacy of fluoxetine (SLC6A4, TPH1, ABCB1). Moreover, decreased/no function SNVs of CYP2D6 (rs1065852, rs28371703, rs1135840) and CYP2C19 (rs12769205) were confirmed phenotypically. Our research contributes to deepening the catalog of genotype-phenotype associations in pharmacokinetics, aiming to increase pharmacogenomics knowledge for rational treatment schemes of antidepressants.
What Are the Impacts of Urbanisation on Carbon Emissions Efficiency? Evidence from Western China
Authors: Yang Le , Liang Zhongqi , Yao Wentao , Zhu Hongmin , Zeng Liangen , Zhao Zihan
Published: September 2023

The contributions of this research are making the theoretical analysis of the impact mechanism of urbanisation on carbon emissions efficiency (CEE), and then empirically analysing the effects of urbanisation on CEE in Western China from 2010 to 2019 by applying the super-efficiency epsilon-based measure model with undesirable outputs and the Tobit model. Major findings are: (1) The mean CEE of the 11 western provinces show a trend of declining and then increasing over those 10 years, demonstrating U-shaped change characteristics. (2) The CEE of Guangxi has the most significant decrease, while the CEE of Chongqing showed only a small fluctuation; the CEE of Guizhou has the characteristic that first it rises, then descends, then rises again; the CEEs of Inner Mongolia and Yunnan have been in the production frontier surface from 2010 to 2015, and presents the trend of declining, then increasing after 2015. The CEEs of the other six western provinces present a trend of declining, then increasing. (3) Chongqing, Sichuan, Inner Mongolia, and Yunnan have a high level of CEE, followed by Shaanxi, Xinjiang, Guangxi, and Guizhou, with an intermediate level of CEE, and Gansu, Ningxia, and Qinghai, with the lowest level of CEE. (4) The effects of urbanisation on CEE in Western China present a U-shaped relationship, meaning that the process of influence was first inhibition, then stimulation. At present, the process of urbanisation shows negative impacts on the CEE, while the tipping point has not yet arrived. The western provinces of China should follow the path of high-quality urbanisation to quickly shift the impact of urbanisation on CEE to the right side of the U-shaped curve.
Circuits and Biomarkers of the Central Nervous System Relating to Astronaut Performance: Summary Report for a NASA-Sponsored Technical Interchange Meeting
Authors: Alwood Joshua S. , Mulavara Ajitkumar P. , Iyer Janani , Mhatre Siddhita D. , Rosi Susanna , Shelhamer Mark , Davis Catherine , Jones Christopher W. , Mao Xiao Wen , Desai Rajeev I. , Whitmire Alexandra M. , Williams Thomas J.
Published: September 2023

Biomarkers, ranging from molecules to behavior, can be used to identify thresholds beyond which performance of mission tasks may be compromised and could potentially trigger the activation of countermeasures. Identification of homologous brain regions and/or neural circuits related to operational performance may allow for translational studies between species. Three discussion groups were directed to use operationally relevant performance tasks as a driver when identifying biomarkers and brain regions or circuits for selected constructs. Here we summarize small-group discussions in tables of circuits and biomarkers categorized by (a) sensorimotor, (b) behavioral medicine and (c) integrated approaches (e.g., physiological responses). In total, hundreds of biomarkers have been identified and are summarized herein by the respective group leads. We hope the meeting proceedings become a rich resource for NASA’s Human Research Program (HRP) and the community of researchers.
Photothermal Imaging of Transient and Steady State Convection Dynamics in Primary Alkanes
Authors: Dominguez Lopez Johan , Gealy Mark W. , Ulness Darin J.
Published: September 2023

This paper presents a photothermal spectroscopy technique that effectively images convective heat flow in molecular liquids resulting from localized laser-induced heating. The method combines aspects of thermal lensing and photothermal deflection. A high-energy infrared laser is used to induce a thermal lens in the sample, and a divergent visible laser is used to probe the entire region of the excitation beam within the sample. This approach allows for the observation of the convective flow of the liquid above the excitation beam. The study focuses on the liquid primary alkanes, from n-pentane to n-pentadecane. The paper provides experimental results, including dynamical data for the propagation of the thermal plume, a transient feature, in these alkanes and the exploration of dependence on excitation laser power.
Predicting Friction of Tapered Roller Bearings with Detailed Multi-Body Simulation Models
Authors: Wingertszahn Patrick , Koch Oliver , Maccioni Lorenzo , Concli Franco , Sauer Bernd
Published: September 2023

In the presented work, a parametric multibody simulation model is presented that is capable of predicting the friction torque and kinematics of tapered roller bearings. For a highly accurate prediction of bearing friction, consideration of solid and lubricant friction is mandatory. For tapered roller bearings in particular, the friction in the contact between the rolling element and raceway is of importance. Friction forces in the contact between the rolling element end face and inner ring rib as well as roller cage pocket contacts are also considered in the model. A large number of tests were carried out to validate the model in terms of the simulated frictional torque. Influencing variables such as speed, axial load, radial load, and temperature were investigated. The simulation results show good agreement with the measured friction torque, which confirms that the model is well suited to predict frictional torques and therefore the kinematics of tapered roller bearings.
Evaluation of Fracture Resistance of Occlusal Veneers Made of Different Types of Materials Depending on Their Thickness
Authors: Czechowski Łukasz , Dejak Beata , Konieczny Bartłomiej , Krasowski Michał
Published: September 2023

Pathological tooth wear is an escalating social problem. Occlusal veneers can be an alternative to traditional prosthetic restorations such as crowns, inlays, and onlays. Background: The aim of this study is to assess the fracture resistance of occlusal veneers made of various materials depending on their thickness. Methods: In total, 120 occlusal veneers were examined. The restorations were made of four ceramics: leucite LC (IPS Empress Esthetic), hybrid HC (Vita Enamic), lithium disilicate LDC (IPS e.max Press), and zirconium oxide ZOC (Ceramill Zolid HT). A total of 30 veneers were made of each material, 10 for each of the three thicknesses: 1 mm, 1.5 mm, 2 mm. The restorations were cemented on identical abutments duplicated from the developed phantom tooth 35 (KaVo) with composite cement (All Bond Universal). The samples prepared in this way were subjected to a compressive strength test in a universal testing machine. Statistical analysis of the results was performed. Results: The average fracture resistance of occlusal veneers made of zirconium oxide ceramic was 1086–1640 N, of lithium disilicate ceramics 456–1044 N, of hybrid ceramics 449–576 N, and of leucite ceramics 257–499 N. Conclusions: Occlusal veneers made of ceramics, zirconium oxide and lithium disilicate, had the highest resistance to fractures. Restorations made of leucite ceramics turned out to be the least resistant to forces. The greater the thickness of the ceramic occlusal veneers, the greater their fracture resistance.