bluuebunny/crossref_metadata_2025 huggingface dataset:
An 1H‐MRS framework predicts the onset of Alzheimer's disease symptoms in PSEN1 mutation carriers
Authors: Londono Ana C. , Castellanos Francisco X. , Arbelaez Andres , Ruiz Adriana , Aguirre‐Acevedo Daniel C. , Richardson Alice M. , Easteal Simon , Lidbury Brett A. , Arcos‐Burgos Mauricio , Lopera Francisco
Published: November 2013

Background

Alzheimer's disease (AD) is the most common cause of dementia; the main risk factors are age and several recently identified genes. A major challenge for AD research is the early detection of subjects at risk. The aim of this study is to develop a predictive model using proton magnetic resonance spectroscopy (1H‐MRS), a noninvasive technique that evaluates brain chemistry in vivo, for monitoring the clinical outcome of carriers of a fully penetrant mutation that causes AD.

Methods

We studied 75 subjects from the largest multigenerational pedigree in the world (∼5000 people) that segregates a unique form of early‐onset Alzheimer's disease (EOAD) caused by a fully penetrant mutation in the Presenilin‐1 gene (PSEN1 p.Glu280Ala [E280 A]). Forty‐four subjects were carriers of the mutation, and 31 were noncarriers. Seventeen carriers had either mild cognitive impairment (MCI) or early‐stage AD (collectively MCI‐AD). In right and left parietal white mater and parasagittal parietal gray matter (RPPGM and LPPGM) of the posterior cingulate gyrus and precuneus, we measured levels of the brain metabolites N‐acetylaspartate (NAA), inositol (Ins), choline (Cho), and glutamate‐glutamine complex (Glx) relative to creatine (Cr) levels (NAA/Cr, Ins/Cr, Cho/Cr, and Glx/Cr, respectively) with two‐dimensional 1H‐MRS. Using advanced recursive partition analysis and random forest analysis, we built classificatory decision trees for both mutation carrier status and the presence of MCI‐AD symptoms, fitting them to 1H‐MRS data while controlling for age, educational level, and sex.

Results

We found that (1) the combination of LPPGM Cho/Cr <0.165 and RPPGM Glx/Cr >1.54 fully excluded carriers; (2) LPPGM Cho/Cr >0.165, RPPGM Glx/Cr <1.54, and left parietal white mater NAA/Cr >1.16 identified asymptomatic carriers with sensitivity of 97.7% and specificity of 77.4%; and (3) RPPGM NAA/Cr >1.05 defined asymptomatic subjects (independent of carrier status) with sensitivity of 100% and a specificity of 96.6%.

Conclusions

Brain metabolites measured by 1H‐MRS in the posterior cingulate gyrus and precuneus are optimally sensitive and specific potential noninvasive biomarkers of subclinical emergence of AD caused by the PSEN1 p.Glu280Ala (E280 A) mutation.
Origin of the PSEN1 E280A mutation causing early‐onset Alzheimer's disease
Authors: Lalli Matthew A. , Cox Hannah C. , Arcila Mary L. , Cadavid Liliana , Moreno Sonia , Garcia Gloria , Madrigal Lucia , Reiman Eric M. , Arcos‐Burgos Mauricio , Bedoya Gabriel , Brunkow Mary E. , Glusman Gustavo , Roach Jared C. , Hood Leroy , Kosik Kenneth S. , Lopera Francisco
Published: November 2013

Background

A mutation in presenilin 1 (E280A) causes early‐onset Alzheimer's disease. Understanding the origin of this mutation will inform medical genetics.

Methods

We sequenced the genomes of 102 individuals from Antioquia, Colombia. We applied identity‐by‐descent analysis to identify regions of common ancestry. We estimated the age of the E280A mutation and the local ancestry of the haplotype harboring this mutation.

Results

All affected individuals share a minimal haplotype of 1.8 Mb containing E280A. We estimate a time to most recent common ancestor of E280A of 10 (95% credible interval, 7.2–12.6) generations. We date the de novo mutation event to 15 (95% credible interval, 11–25) generations ago. We infer a western European geographic origin of the shared haplotype.

Conclusions

The age and geographic origin of E280A are consistent with a single founder dating from the time of the Spanish Conquistadors who began colonizing Colombia during the early 16th century.
Cerebrospinal fluid BACE1 activity and markers of amyloid precursor protein metabolism and axonal degeneration in Alzheimer's disease
Authors: Perneczky Robert , Alexopoulos Panagiotis ,   Alzheimer's Disease euroimaging Initiative
Published: November 2013

Objective

The objective of this study was to assess cerebrospinal fluid (CSF) β‐site amyloid precursor protein (APP)‐cleaving enzyme 1 (BACE1) activity in relation to Alzheimer's disease (AD) and to correlate the enzyme activity with protein markers of APP metabolism and axonal degeneration.

Methods

BACE1 activity and protein concentrations were measured and analyzed in 342 participants of the Alzheimer's Disease Neuroimaging Initiative, including 99 normal control, 75 stable mild cognitive impairment (MCI), 87 progressive MCI, and 79 AD dementia cases. All statistical analyses were Bonferroni corrected for multiple comparisons.

Results

No significant differences between controls and any of the three patient groups were detected for BACE1 activity and soluble APPβ (sAPPβ) concentrations in CSF. Significant correlations with BACE1 activity were found for CSF APPβ and total tau in all four groups and for CSF phosphorylated tau181 in all groups but the progressive MCI group. There were no correlations for CSF amyloid β (Aβ)1‐42 or for plasma Aβ1‐42 and Aβ1‐40.

Conclusions

The consistent correlation between BACE1 activity and sAPPβ supports their role as biomarkers of target engagement in clinical trials on BACE1 inhibition.
Analysis of coprolites from the extinct mountain goat Myotragus balearicus
Authors: Welker Frido , Duijm Elza , van der Gaag Kristiaan J. , van Geel Bas , de Knijff Peter , van Leeuwen Jacqueline , Mol Dick , van der Plicht Johannes , Raes Niels , Reumer Jelle , Gravendeel Barbara
Published: November 2013

Humans colonized the Balearic Islands 5–4 ka ago. They arrived in a uniquely adapted ecosystem with the Balearic mountain goat Myotragus balearicus (Bovidae, Antilopinae, Caprini) as the only large mammal. This mammal went extinct rapidly after human arrival. Several hypotheses have been proposed to explain the extinction of M. balearicus. For the present study ancient DNA analysis (Sanger sequencing, Roche-454, Ion Torrent), and pollen and macrofossil analyses were performed on preserved coprolites from M. balearicus, providing information on its diet and paleo-environment. The information retrieved shows that M. balearicus was heavily dependent on the Balearic box species Buxus balearica during at least part of the year, and that it was most probably a browser. Hindcast ecological niche modelling of B. balearica shows that local distribution of this plant species was affected by climate changes. This suggests that the extinction of M. balearicus can be related to the decline and regional extinction of a plant species that formed a major component of its diet. The vegetation change is thought to be caused by increased aridity occurring throughout the Mediterranean. Previous hypotheses relating the extinction of M. balearicus directly to the arrival of humans on the islands must therefore be adjusted.
Prevalence and Predictors of Post-traumatic Stress Disorder in Adults One Year Following Traumatic Brain Injury: A Population-based Study
Authors: Barker-Collo Suzanne , Theadom Alice , Ameratunga Shanthi , Jones Kelly , Jones Amy , Starkey Nicola , Feigin Valery L.
Published: November 2013

Objective: Experiencing a traumatic brain injury (TBI) may increase the risk of post-traumatic stress disorder (PTSD); however, this has not been examined in a population-based context. This study examined prevalence and predictors (demographic and injury related) of PTSD 1 year after TBI in a population-based sample.

Method: A population-based TBI incidence and outcomes study was conducted in Hamilton and Waikato Districts of New Zealand over 12 months (February 2011–March 2012) with follow-up at 1 year. The relationship of baseline demographic and TBI characteristics to PTSD (Post-traumatic Diagnostic Scale; PDS) was examined in 431 adults (>15 years old) 1 year post-TBI.

Results: On average, participants reported three PTSD symptoms, with heightened arousal being the most commonly reported. Nearly 18% of participants met criteria for PTSD. These participants were significantly more likely to report pre-TBI history of depression/anxiety, and to have drugs implicated in the injury. Regression indicated that female gender, increased loss of consciousness (LOC) and intentional injury predicted PTSD severity (R2= .109,F[6, 294] = 6.007,p< .001) and number of symptoms (R2= .098,F[3, 297] = 4.562,p< .001).

Conclusion: PTSD occurred as a long-term sequela in 18% of participants after TBI, with increased PTSD severity and greater number of symptoms associated with female gender, longer LOC and intentional injury. These findings have implications for identification and targeting of assessment and intervention resources towards those at greatest risk of PTSD following TBI.
Indigenous Theory Building for Māori Children and Adolescents with Traumatic Brain Injury and their Extended Family
Authors: Elder Hinemoa
Published: November 2013

Background: International research identifies indigeneity as a risk factor for traumatic brain injury (TBI). Aotearoa New Zealand studies show that mokopuna (grandchildren; used here to encompass the ages and stages of infant, child and adolescent development and those in young adulthood) are significantly overrepresented in TBI populations. The important role of whānau (family) is also well established in child and adolescent TBI scholarship. Despite awareness of these factors, no studies have been identified that explore whānau knowledge about mokopuna TBI. The aim of this study was to explore two questions: (1) What do Māori people say about mokopuna TBI in the context of the Māori cultural belief that the head is the most sacred part of the body? and (2) How could this information be used to build theory that could inform addressing the rehabilitation needs of this group?

Method: Eighteen marae wānanga (culture-specific fora in traditional meeting houses) were held. The wānanga typically lasted approximately 2 hours. Footage and written transcripts were analysed using Rangahau Kaupapa Māori (Māori indigenous research methods).

Results: The wairua theory of mokopuna TBI proposes that TBI not only injures brain anatomy and physiology but also injures wairua (defined here as a unique connection between Māori and all aspects of the universe). Injury to wairua means that culturally determined interventions are both indicated and expected. The wairua theory of mokopuna TBI thereby provides a guide to intervention.

Conclusion: A Māori theory of mokopuna TBI has been identified which describes a culture-specific aspect of TBI. This theory proposes that pre-existing whānau knowledge salient to TBI is critical to optimising recovery. Further research is needed to test this theory not only in TBI but also in other areas such as in mental illness, neurodegenerative disease and addiction.
POLICING EPISTEMIC COMMUNITIES
Authors: Bruner Justin P.
Published: November 2013

I examine how particular social arrangements and incentive structures encourage the honest reporting of experimental results and minimize fraudulent scientific work. In particular I investigate how epistemic communities can achieve this goal by promoting members to police the community. Using some basic tools from game theory, I explore a simple model in which scientists both conduct research and have the option of investigating the findings of their peers. I find that this system of peer policing can in many cases ensure high levels of honesty.
SCIENTIFIC NETWORKS ON DATA LANDSCAPES: QUESTION DIFFICULTY, EPISTEMIC SUCCESS, AND CONVERGENCE
Authors: Grim Patrick , Singer Daniel J. , Fisher Steven , Bramson Aaron , Berger William J. , Reade Christopher , Flocken Carissa , Sales Adam
Published: November 2013

A scientific community can be modeled as a collection of epistemic agents attempting to answer questions, in part by communicating about their hypotheses and results. We can treat the pathways of scientific communication as a network. When we do, it becomes clear that the interaction between the structure of the network and the nature of the question under investigation affects epistemic desiderata, including accuracy and speed to community consensus. Here we build on previous work, both our own and others’, in order to get a firmer grasp on precisely which features of scientific communities interact with which features of scientific questions in order to influence epistemic outcomes.

Here we introduce a measure on the landscape meant to capture some aspects of the difficulty of answering an empirical question. We then investigate both how different communication networks affect whether the community finds the best answer and the time it takes for the community to reach consensus on an answer. We measure these two epistemic desiderata on a continuum of networks sampled from the Watts–Strogatz spectrum. It turns out that finding the best answer and reaching consensus exhibit radically different patterns. The time it takes for a community to reach a consensus in these models roughly tracks mean path length in the network. Whether a scientific community finds the best answer, on the other hand, tracks neither mean path length nor clustering coefficient.
Improvement of facial affect recognition in children and adolescents with attention-deficit/hyperactivity disorder under methylphenidate
Authors: Beyer von Morgenstern Sophie , Becker Ingrid , Sinzig Judith
Published: November 2013

Introduction and Hypothesis

Some authors draw a connection between the dopaminergic pathways and emotional perception. The present study is based on that association and addresses the question whether methylphenidate and the resulting amelioration of the disturbed dopamine metabolism lead to an improvement of the facial affect recognition abilities in children with attention-deficit/hyperactivity disorder (ADHD).

Methods

A computer test was conducted on 21 participants, aged 7–14 years and with a diagnosis of ADHD – some with comorbid oppositional defiant disorder – conducted the FEFA (Frankfurt Test and Training of Facial Affect), a computer test to examine their facial affect recognition abilities. It consists of two subtests, one with faces and one with eye pairs. All participants were tested in a double-blind cross-over study, once under placebo and once under methylphenidate.

Results and Discussion

The collected data showed that methylphenidate leads to amelioration of facial affect recognition abilities, but not on a significant level. Reasons for missing significance may be the small sample size or the fact that there exists some overlapping in cerebral connections and metabolic pathways of the site of action of methylphenidate and the affected dopaminergic areas in ADHD. However, consistent with the endophenotype concept, certain gene locations of the dopaminergic metabolism as both an aetiological factor for ADHD and the deficient facial affect recognition abilities with these individuals were considered. Consulting current literature they were found to be not concordant. Therefore, we conclude that the lacking significance of the methylphenidate affect on facial affect recognition is based on this fact.
mRNA Expression of lipogenic enzymes in mammary tissue and fatty acid profile in milk of dairy cows fed flax hulls and infused with flax oil in the abomasum
Authors: Palin Marie-France , Côrtes Cristiano , Benchaar Chaouki , Lacasse Pierre , Petit Hélène V.
Published: November 2013

In the present study, the effect of flax hulls with or without flax oil bypassing the rumen on the expression of lipogenic genes in the mammary tissue of dairy cows was investigated. A total of eight dairy cows were used in a replicated 4 × 4 Latin square design. There were four periods of 21 d each and four treatments: control diet with no flax hulls (CONT); diet with 9·88 % flax hulls in the DM (HULL); control diet with 500 g flax oil/d infused in the abomasum (COFO); diet with 9·88 % flax hulls in the DM and 500 g flax oil/d infused in the abomasum (HUFO). A higher mRNA abundance of sterol regulatory element binding transcription factor, fatty acid (FA) synthase, lipoprotein lipase (LPL), PPARγ1, stearoyl-CoA desaturase (SCD) and acetyl-coenzyme A carboxylase-α was observed in cows fed HULL than in those fed CONT, and HUFO had the opposite effect. Compared with CONT, COFO and HUFO lowered the mRNA abundance of SCD, which may explain the lower proportions of MUFA in milk fat with flax oil infusion. The mRNA abundance of LPL in mammary tissue and proportions of long-chain FA in milk fat were higher in cows fed COFO than in those fed CONT. The highest proportions of trans FA were observed when cows were fed HULL. The present study demonstrates that flax hulls with or without flax oil infusion in the abomasum can affect the expression of lipogenic genes in the mammary tissue of dairy cows, which may contribute to the improvement of milk FA profile.
Metabolic benefits of dietary prebiotics in human subjects: a systematic review of randomised controlled trials
Authors: Kellow Nicole J. , Coughlan Melinda T. , Reid Christopher M.
Published: November 2013

Complex relationships exist between the gut microflora and their human hosts. Emerging evidence suggests that bacterial dysbiosis within the colon may be involved in the pathogenesis of the metabolic syndrome, type 2 diabetes and CVD. The use of dietary prebiotic supplements to restore an optimal balance of intestinal flora may positively affect host metabolism, representing a potential treatment strategy for individuals with cardiometabolic disorders. The present review aimed to examine the current evidence supporting that dietary prebiotic supplementation in adults has beneficial effects on biochemical parameters associated with the development of metabolic abnormalities including obesity, glucose intolerance, dyslipidaemia, hepatic steatosis and low-grade chronic inflammation. Between January 2000 and September 2013, eight computer databases were searched for randomised controlled trials published in English. Human trials were included if at least one group received a dietary prebiotic intervention. In the present review, twenty-six randomised controlled trials involving 831 participants were included. Evidence indicated that dietary prebiotic supplementation increased self-reported feelings of satiety in healthy adults (standardised mean difference − 0·57, 95 % CI − 1·13, − 0·01). Prebiotic supplementation also significantly reduced postprandial glucose ( − 0·76, 95 % CI − 1·41, − 0·12) and insulin ( − 0·77, 95 % CI − 1·50, − 0·04) concentrations. The effects of dietary prebiotics on total energy intake, body weight, peptide YY and glucagon-like peptide-1 concentrations, gastric emptying times, insulin sensitivity, lipids, inflammatory markers and immune function were contradictory. Dietary prebiotic consumption was found to be associated with subjective improvements in satiety and reductions in postprandial glucose and insulin concentrations. Additional evidence is required before recommending prebiotic supplements to individuals with metabolic abnormalities. Large-scale trials of longer duration evaluating gut microbial growth and activity are required.
Policy Representation, Social Representation and Class Voting in Britain
Authors: Heath Oliver
Published: November 2013

Why does the strength of class voting vary over time? Recent research has emphasized factors related to the structure of political choice at the party level. This article examines different aspects of this choice, and investigates whether voters are more likely to respond to the social or policy cues that parties send voters. The results from the British context suggest that the former are more important than the latter. The central implication of this finding is that social representation matters, and that the social background of political representatives influences how voters relate to political parties.
The Politics of Police Image in Chile
Authors: BONNER MICHELLE D.
Published: November 2013

abstract

The Carabineros de Chile played an active role in the repression and leadership of the Pinochet regime, yet today, the police force is one of the most respected institutions in Chile. Few post-authoritarian democracies have been able to restore such esteem for their police as quickly. But what does respect mean? This study analyses the narratives of approximately 50 police experts in Chile. It finds that police experts regard the positive image of the Carabineros as having more to do with fear and an effective communications strategy than police reform. It is important to understand how those Chileans most involved in policy discussions on policing interpret the reasons for respect, as their interpretations can shape the types of policing advocated or practised.
Building Democracy or Reproducing ‘Ecuadoreanness’? A Transnational Exploration of Ecuadorean Migrants' External Voting
Authors: BOCCAGNI PAOLO , RAMÍREZ JACQUES
Published: November 2013

Ecuador has emerged as a good case study of the interactions between diasporas and their countries of origin. The recent enactment of external voting rights has mirrored a novel political discourse which emphasises the positive contribution of ‘emigrant brothers’ and their unbroken allegiance to their homeland. Expatriate reactions to these new political developments are at the core of our article, which heuristically reconstructs the social roots and meanings of expatriate participation in the constitutional referendum held in 2008. Questionnaires were simultaneously administered to Ecuadorean voters in nine cities in seven countries. Based on this innovative convenience sample, the expectations and motivations underlying people's electoral involvement and civic participation are explored against the background of transnational connections and attachments. An understanding of voters' involvement on a terrain of symbolic patriotism, identity reassertion and ‘home re-evocation’ – rather than in strictly electoral terms – is advanced. External voting – whatever its impact on domestic politics – should be appreciated as an institutional opportunity for migrants' national identification and belonging to be represented.
The influence of school holiday timing on epidemic impact
Authors: EAMES K. T. D.
Published: November 2013

The impact of reactive school closure on an epidemic is uncertain, since it is not clear how an unplanned closure will affect social mixing patterns. The effect of school holidays on social mixing patterns is better understood. Here, we use mathematical models to explore the influence of the timing of school holidays on the final size and peak incidence of an influenza-like epidemic. A well-timed holiday can reduce the impact of an epidemic, in particular substantially reducing an epidemic's peak. Final size and peak incidence cannot both be minimized: a later holiday is optimal for minimizing the final size, while an earlier holiday minimizes peak incidence. Using social mixing data from the UK, we estimated that, had the 2009 influenza epidemic not been interrupted by the school summer holidays, the final size would have been about 20% larger and the peak about 170% higher.
JONATHAN SWIFT ON THE LIVES OF THE POOR NATIVE IRISH AS SEEN THROUGH
“A MODEST PROPOSAL” AND OTHER OF HIS WRITINGS
Authors: Welch Patrick
Published: November 2013

This article focuses on Jonathan Swift’s “A Modest
Proposal” and other of his writings that were prompted by the
unsustainable socio-economic and geo-political conditions under which the poor
native Irish were living. The article begins with introductory comments and then
moves to conditions in Ireland when Swift was writing that contributed to his
strong concern for the native poor. Next is provided a summary of “A
Modest Proposal” and consideration of what drove Swift to the extreme
he reached in the essay. Following this, Swift’s writings are
explored as indicators of his opinion of political arithmeticians, and
conclusions close the paper.
ON VIRTUES AND AWARDS: GIACINTO DRAGONETTI AND THE TRADITION OF ECONOMIA CIVILE IN ENLIGHTENMENT ITALY
Authors: BRUNI LUIGINO
Published: November 2013

The paper discusses the work of Giacinto Dragonetti, a disciple of the Neapolitan Antonio Genovesi, founder of the Economia Civile tradition. Dragonetti’s short book, A Treatise on Virtues and Rewards, appeared in Naples in 1766, shortly after Beccaria’s On Crimes and Punishments (1764). In the Treatise, Dragonetti advances a theory of action based on awards for virtues. The idea of awards relies on the hypothesis that good or virtuous citizens act for intrinsic reasons. Modern economics has followed the path of incentives (and “punishments”), not that of awards. The paper argues that Dragonetti’s contributions remain relevant not only for the history of economic thought, but for contemporary economic theory, as well.
Linguistic and cognitive motivations for the Typological Primacy Model (TPM) of third language (L3) transfer: Timing of acquisition and proficiency considered
Authors: ROTHMAN JASON
Published: November 2013

This article elucidates the Typological Primacy Model (TPM; Rothman, 2010, 2011, 2013) for the initial stages of adult third language (L3) morphosyntactic transfer, addressing questions that stem from the model and its application. The TPM maintains that structural proximity between the L3 and the L1 and/or the L2 determines L3 transfer. In addition to demonstrating empirical support for the TPM, this article articulates a proposal for how the mind unconsciously determines typological (structural) proximity based on linguistic cues from the L3 input stream used by the parser early on to determine holistic transfer of one previous (the L1 or the L2) system. This articulated version of the TPM is motivated by argumentation appealing to cognitive and linguistic factors. Finally, in line with the general tenets of the TPM, I ponder if and why L3 transfer might obtain differently depending on the type of bilingual (e.g. early vs. late) and proficiency level of bilingualism involved in the L3 process.
FIB Plan and Side View Cross-Sectional TEM Sample Preparation of Nanostructures
Authors: Lenrick Filip , Ek Martin , Jacobsson Daniel , Borgström Magnus T. , Wallenberg L. Reine
Published: November 2013

Focused ion beam is a powerful method for cross-sectional transmission electron microscope sample preparation due to being site specific and not limited to certain materials. It has, however, been difficult to apply to many nanostructured materials as they are prone to damage due to extending from the surface. Here we show methods for focused ion beam sample preparation for transmission electron microscopy analysis of such materials, demonstrated on GaAs–GaInP core shell nanowires. We use polymer resin as support and protection and are able to produce cross-sections both perpendicular to and parallel with the substrate surface with minimal damage. Consequently, nanowires grown perpendicular to the substrates could be imaged both in plan and side view, including the nanowire–substrate interface in the latter case. Using the methods presented here we could analyze the faceting and homogeneity of hundreds of adjacent nanowires in a single lamella.
Habermas, Religion, and Citizenship
Authors: Neal Patrick
Published: November 2013

What is the appropriate place for religious argument in the public realm of a liberal-democratic polity? The primary competing positions have been a “liberal” account and a “revisionist” response arguing for a greater role for religious argument in liberal democracy than the liberal position is ordinarily understood to allow. Liberals and their revisionist critics disagree about whether restraints on religious arguments and justifications are justified and desirable. Jürgen Habermas has intervened in this debate with a provocative account of the place of religion in the public sphere. Habermas presents his account as an alternative to both the liberal and the revisionist perspectives, and purports to do justice to the legitimate claims of each without falling prey to the failings of either. This article critically analyzes Habermas's interesting proposal and argues that it does not succeed.
Psychometric Evaluation of the Sociocultural Attitudes Towards Appearance Questionnaire-3 among Brazilian Young Adults
Authors: Amaral Ana C. S. , Ribeiro Mário S. , Conti Maria A. , Ferreira Clécio S. , Ferreira Maria E. C.
Published: November 2013

The objective was evaluating the psychometric properties of the Sociocultural Attitudes Towards Appearance Questionnaire-3 (SATAQ–3) among Brazilian young adults of both genders. The sample was composed by 506 undergraduate students (295 females and 211 males), aged between 17 and 29 years old. Exploratory and confirmatory factor analyses were used for construct validity (N = 506). Correlations between the SATAQ–3 scores and those of the Tripartite Influence Scale (TIS) and Body Shape Questionnaire (BSQ) were used for convergent validity. Reliability was assessed through internal consistency (α) and reproducibility (test-retest) through comparison of the means obtained at two different time points and through intra-class correlation. The scale presented a factor structure composed of five factors, replicated in the confirmatory factor analysis with satisfactory values for the measurements of adjustment to the model. Correlations with the BSQ and TIS scores were rho = .52 and rho = –.35, respectively. Cronbach’s alpha coefficients were satisfactory, and their stability was demonstrated. Brazilian SATAQ–3 had good validity and reproducibility, being indicated for use in samples of Brazilian youths.
Spanish Version of the Meta-Cognitions Questionnaire 30 (MCQ-30)
Authors: Ramos-Cejudo Juan , Salguero José M. , Cano-Vindel Antonio
Published: November 2013

The Meta-cognitions Questionnaire 30 (MCQ-30; Wells & Cartwright-Hatton, 2004) has been used to assess individual differences in metacognitive beliefs considered to be central in the metacognitive theory of generalized anxiety disorder (Wells, 2005). In the present study, the psychometric properties, reliability, and validity of a Spanish adaptation of the MCQ-30 were tested in a broad sample of participants (N = 768) of Spanish nationality aged 16–81 years (31.1% males, 68.9% females). Confirmatory factor analysis showed the expected five-factor structure, which was found to be invariant across gender. Internal consistency and test-retest reliability of the subscales were adequate, and the expected relationships to theoretically related variables such as pathological worry, meta-worry, thought suppression and trait anxiety were obtained. Convergent validity with other measures of beliefs about worry was also found. Taken together, these findings suggest that the Spanish version of the MCQ-30 is a valid instrument for evaluating metacognitive beliefs in the Spanish-speaking population.
Activity of Xenobiotic-Metabolizing Enzymes in the Liver of Rats with Multi-Vitamin Deficiency
Authors: Tutelyan Victor A. , Kravchenko Lidia V. , Aksenov Ilya V. , Trusov Nikita V. , Guseva Galina V. , Kodentsova Vera M. , Vrzhesinskaya Oksana A. , Beketova Nina A.
Published: November 2013

The purpose of the study was to determine how multi-vitamin deficiency affects xenobiotic-metabolizing enzyme (XME) activities in the rat liver. Vitamin levels and XME activities were studied in the livers of male Wistar rats who were fed for 4 weeks with semi-synthetic diets containing either adequate (100 % of recommended vitamin intake) levels of vitamins (control), or decreased vitamin levels (50 % or 20 % of recommended vitamin intake). The study results have shown that moderate vitamin deficiency (50 %) leads to a decrease of vitamin A levels only, and to a slight increase, as compared with the control, in the following enzyme activities: methoxyresorufin O-dealkylase (MROD) activity of CYP1 A2 - by 34 % (p < 0.05), UDP-glucuronosyl transferase - by 26 % (p < 0.05), and quinone reductase - by 55 % (p < 0.05). Profound vitamin deficiency (20 %) led to a decrease of vitamins A, E, B1, B2, and C, and enzyme activities in the liver: MROD - to 78 % of the control level (p < 0.05), 4-nitrophenol hydroxylase - to 74 % (p < 0.05), heme oxygenase-1 - to 83 % (p < 0.05), and quinone reductase - to 60 % (p < 0.05). At the same time, the UDP-glucuronosyl transferase activity and ethoxyresorufin O-dealkylase activity of CYP1A1, pentoxyresorufin O-dealkylase activity of CYP2B1/2 and 6β-testosterone hydroxylase, as well as the total activity of glutathione transferase did not differ from the control levels. The study has demonstrated that profound multi-vitamin deficiency is associated with a decrease in the expression of CYP1A2 and CYP3A1 mRNAs to 62 % and 79 %, respectively. These data indicated that a short-term but profound multi-vitamin deficiency in rats leads to a decrease in the activities and expression of the some XME that play an important role in detoxification of xenobiotics and metabolism of drugs and antioxidant protection.
The Impact of Prenatal Vitamin A and Zinc Supplementation on Birth Size and Neonatal Survival - a Double-Blind, Randomized Controlled Trial in a Rural Area of Indonesia
Authors: Prawirohartono Endy P. , Nyström Lennarth , Nurdiati Detty S. , Hakimi Mohammad , Lind Torbjörn
Published: November 2013

Background: Prenatal supplementation with micronutrients may increase birth weight and thus improve infant health and survival in settings where infants and children are at risk of micronutrient deficiencies. Objective: To assess whether vitamin A and/or zinc supplementation given during pregnancy can improve birth weight, birth length, neonatal morbidity, or infant mortality. Methods: A double-blind, randomized controlled trial supplementing women (n = 2173) in Central Java, Indonesia throughout pregnancy with vitamin A, zinc, combined vitamin A+zinc, or placebo. Results: Out of 2173 supplemented pregnant women, 1956 neonates could be evaluated. Overall, zinc supplementation improved birth length compared to placebo or combined vitamin A+zinc (48.8 vs. 48.5 cm, p = 0.04); vitamin A supplementation improved birth length compared to placebo or combined vitamin A+zinc (48.7 vs. 48.2 cm, p = 0.04). These effects remained after adjusting for maternal height, pre-pregnancy weight, and parity. There was no effect of supplementation on birth weight, the proportion of low birth weight, neonatal morbidity, or mortality. Conclusions: Prenatal zinc or vitamin A supplementation demonstrates a small but significant effect on birth length, but supplementation with zinc, vitamin A or a combination of zinc and vitamin A, have no effect on birth weight, neonatal morbidity, or mortality.
Omega 3 and Omega 6 Fatty Acids Intake and Dietary Sources in a Representative Sample of Spanish Adults
Authors: González-Rodríguez Liliana G. , Aparicio Aránzazu , López-Sobaler Ana M. , Ortega Rosa M.
Published: November 2013

The present study analyzes the intake of omega 3 (n-3 PUFAs) and omega 6 (n-6 PUFAs) and dietary sources in a representative sample of Spanish adults. For this purpose 418 adults (18 - 60 y), from 15 Spanish provinces were studied. The intake of energy and nutrients [specifically, the n-3 polyunsaturated fatty acids (PUFAs,) α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA); and the n-6 PUFA, linoleic acid (LA)] was determined using a 24-hour recall questionnaire for two days. The Multiple Source Method (MSM) was used to estimate participants usual fatty acid intake. The total n-3 PUFAs intake was 1.8 ± 0.60 g/day (ALA: 1.3 ± 0.32, EPA: 0.16 ± 0.14, and DHA: 0.33 ± 0.21 g/day) and n-6 PUFA intake was 11.0 ± 2.7 g/day (LA: 10.8 ± 2.7 g/day). A high proportion of participants did not meet their nutrient intake goals for total n-3 PUFAs (84.7 %), ALA (45.0 %), and EPA plus DHA (62.9 %). The main food sources for ALA were oil, dairy products, and meat; for EPA fish; for DHA, fish, eggs, and meat; and for LA, oils, meat, and cereals. Therefore, an increase in the intake of foods rich in n-3 PUFAs or the use of supplements with n-3 PUFAs might help to improve the n-3 PUFA intake.
Alpha-linolenic acid intake prevents endothelial dysfunction in high-fat diet-fed streptozotocin rats and underlying mechanisms
Authors: Zhang Wei , Fu Fang , Tie Ru , Liang Xiangyan , Tian Fei , Xing Wenjuan , Li Jia , Ji Lele , Xing Jinliang , Sun Xin , Zhang Haifeng
Published: November 2013

Background: Endothelial dysfunction is an important factor in the pathogenesis of diabetes related vascular complications, and acute alpha-linolenic acid (ALA) intake can increase flow-mediated dilation of the diabetic artery at 4 h postprandially. However, whether chronic ALA supplementation may prevent endothelial dysfunction in the process of diabetes and underlying mechanisms remains largely unknown. Materials and methods: The high-fat diet-fed streptozotocin (HFD-STZ) rats provided an animal model for T2DM. Age-matched normal and HFD-STZ rats randomly received normal diet or ALA (500 mg/kg per day). After 5 weeks of feeding, endothelial function was determined. Results: Diabetes caused significant endothelial dysfunction (maximal vasorelaxation responses to ACh) in aortic segments, and ALA intake alleviated endothelial dysfunction. Superoxide production and peroxynitrite (ONOO-) formation were reduced with ALA supplement in diabetic vascular segments. Interestingly, ALA intake enhanced eNOS but inhibited iNOS activity in diabetic vessels. Moreover, ALA intake significantly increased eNOS phosphorylation. On the other hand, gp91phox and iNOS overexpression were reduced moderately with ALA intake in diabetic vessels. Conclusions: We concluded that ALA prevents diabetes-induced endothelial dysfunction by enhancing eNOS activity and attenuates oxidative/nitrative stress by inhibiting iNOS and NADPH oxidase expression and ONOO- production.
Reversal of Metabolic Deficits by Lipoic Acid in a Triple Transgenic Mouse Model of Alzheimer's Disease: A 13C NMR Study
Authors: Sancheti Harsh , Kanamori Keiko , Patil Ishan , Díaz Brinton Roberta , Ross Brian D , Cadenas Enrique
Published: November 2013

Alzheimer's disease is an age-related neurodegenerative disease characterized by deterioration of cognition and loss of memory. Several clinical studies have shown Alzheimer's disease to be associated with disturbances in glucose metabolism and the subsequent tricarboxylic acid (TCA) cycle-related metabolites like glutamate (Glu), glutamine (Gln), and N-acetylaspartate (NAA). These metabolites have been viewed as biomarkers by (a) assisting early diagnosis of Alzheimer's disease and (b) evaluating the efficacy of a treatment regimen. In this study, 13-month-old triple transgenic mice (a mouse model of Alzheimer's disease (3xTg-AD)) were given intravenous infusion of [1-13C]glucose followed by an ex vivo13C NMR to determine the concentrations of 13C-labeled isotopomers of Glu, Gln, aspartate (Asp), GABA, myo-inositol, and NAA. Total (12C+13C) Glu, Gln, and Asp were quantified by high-performance liquid chromatography to calculate enrichment. Furthermore, we examined the effects of lipoic acid in modulating these metabolites, based on its previously established insulin mimetic effects. Total 13C labeling and percent enrichment decreased by ∼50% in the 3xTg-AD mice. This hypometabolism was partially or completely restored by lipoic acid feeding. The ability of lipoic acid to restore glucose metabolism and subsequent TCA cycle-related metabolites further substantiates its role in overcoming the hypometabolic state inherent in early stages of Alzheimer's disease.
Membrane lipids are key modulators of the endocannabinoid-hydrolase FAAH
Authors: Dainese Enrico , De Fabritiis Gianni , Sabatucci Annalaura , Oddi Sergio , Angelucci Clotilde Beatrice , Di Pancrazio Chiara , Giorgino Toni , Stanley Nathaniel , Del Carlo Michele , Cravatt Benjamin F. , Maccarrone Mauro
Published: November 2013

Lipid composition is expected to play an important role in modulating membrane enzyme activity, in particular if the substrates are themselves lipid molecules. A paradigmatic case is FAAH (fatty acid amide hydrolase), an enzyme critical in terminating endocannabinoid signalling and an important therapeutic target. In the present study, using a combined experimental and computational approach, we show that membrane lipids modulate the structure, subcellular localization and activity of FAAH. We report that the FAAH dimer is stabilized by the lipid bilayer and shows a higher membrane-binding affinity and enzymatic activity within membranes containing both cholesterol and the natural FAAH substrate AEA (anandamide). Additionally, co-localization of cholesterol, AEA and FAAH in mouse neuroblastoma cells suggests a mechanism through which cholesterol increases the substrate accessibility of FAAH.
Assessment of charge-transfer excitations with time-dependent, range-separated density functional theory based on long-range MP2 and multiconfigurational self-consistent field wave functions
Authors: Hedegård Erik Donovan , Heiden Frank , Knecht Stefan , Fromager Emmanuel , Jensen Hans Jørgen Aagaard
Published: November 2013

Charge transfer excitations can be described within Time-Dependent Density Functional Theory (TD-DFT), not only by means of the Coulomb Attenuated Method (CAM) but also with a combination of wave function theory and TD-DFT based on range separation. The latter approach enables a rigorous formulation of multi-determinantal TD-DFT schemes where excitation classes, which are absent in conventional TD-DFT spectra (like for example double excitations), can be addressed. This paper investigates the combination of both the long-range Multi-Configuration Self-Consistent Field (MCSCF) and Second Order Polarization Propagator Approximation (SOPPA) ansätze with a short-range DFT (srDFT) description. We find that the combinations of SOPPA or MCSCF with TD-DFT yield better results than could be expected from the pure wave function schemes. For the Time-Dependent MCSCF short-range DFT ansatz (TD-MC-srDFT) excitation energies calculated over a larger benchmark set of molecules with predominantly single reference character yield good agreement with their reference values, and are in general comparable to the CAM-B3LYP functional. The SOPPA-srDFT scheme is tested for a subset of molecules used for benchmarking TD-MC-srDFT and performs slightly better against the reference data for this small subset. Beyond the proof-of-principle calculations comprising the first part of this contribution, we additionally studied the low-lying singlet excited states (S1 and S2) of the retinal chromophore. The chromophore displays multireference character in the ground state and both excited states exhibit considerable double excitation character, which in turn cannot be described within standard TD-DFT, due to the adiabatic approximation. However, a TD-MC-srDFT approach can account for the multireference character, and excitation energies are obtained with accuracy comparable to CASPT2, although using a much smaller active space.
Electrical detection of CF3Cl phase transitions on graphene
Authors: Wang Yilin , Bao Wenzhong , Xiao Shudong , Fuhrer Michael S. , Reutt-Robey Janice
Published: November 2013

The temperature-dependent conductivity of bilayer graphene with adsorbed layers of the halocarbon molecule CF3Cl was studied under ultra high vacuum conditions. Upon warming CF3Cl sub-monolayer from 25 K, the electrical conductivity drops abruptly at 47 K and exhibits additional inflection points at 60 K and 69 K. CF3Cl multi-layers exhibit an abrupt conductivity gain at 54 K. These conductivity features correspond to known temperature-coverage phase boundaries for CF3Cl films measured on graphite. The changes in conductivity reflect changes in dielectric screening and disorder potential of the CF3Cl adlayer. The chemical specificity of phase transitions presents a basis for sensor selectivity.
Effects of substrate temperature, substrate orientation, and energetic atomic collisions on the structure of GaN films grown by reactive sputtering
Authors: Schiaber Ziani S. , Leite Douglas M. G. , Bortoleto José R. R. , Lisboa-Filho Paulo N. , da Silva José H. D.
Published: November 2013

The combined effects of substrate temperature, substrate orientation, and energetic particle impingement on the structure of GaN films grown by reactive radio-frequency magnetron sputtering are investigated. Monte-Carlo based simulations are employed to analyze the energies of the species generated in the plasma and colliding with the growing surface. Polycrystalline films grown at temperatures ranging from 500 to 1000 °C clearly showed a dependence of orientation texture and surface morphology on substrate orientation (c- and a-plane sapphire) in which the (0001) GaN planes were parallel to the substrate surface. A large increase in interplanar spacing associated with the increase in both a- and c-parameters of the hexagonal lattice and a redshift of the optical bandgap were observed at substrate temperatures higher than 600 °C. The results showed that the tensile stresses produced during the film's growth in high-temperature deposition ranges were much larger than the expected compressive stresses caused by the difference in the thermal expansion coefficients of the film and substrate in the cool-down process after the film growth. The best films were deposited at 500 °C, 30 W and 600 °C, 45 W, which corresponds to conditions where the out diffusion from the film is low. Under these conditions the benefits of the temperature increase because of the decrease in defect density are greater than the problems caused by the strongly strained lattice that occurr at higher temperatures. The results are useful to the analysis of the growth conditions of GaN films by reactive sputtering.
Modeling oscillatory dynamics in brain microcircuits as a way to help uncover neurological disease mechanisms: A proposal
Authors: Skinner F. K. , Ferguson K. A.
Published: November 2013

There is an undisputed need and requirement for theoretical and computational studies in Neuroscience today. Furthermore, it is clear that oscillatory dynamical output from brain networks is representative of various behavioural states, and it is becoming clear that one could consider these outputs as measures of normal and pathological brain states. Although mathematical modeling of oscillatory dynamics in the context of neurological disease exists, it is a highly challenging endeavour because of the many levels of organization in the nervous system. This challenge is coupled with the increasing knowledge of cellular specificity and network dysfunction that is associated with disease. Recently, whole hippocampus in vitro preparations from control animals have been shown to spontaneously express oscillatory activities. In addition, when using preparations derived from animal models of disease, these activities show particular alterations. These preparations present an opportunity to address challenges involved with using models to gain insight because of easier access to simultaneous cellular and network measurements, and pharmacological modulations. We propose that by developing and using models with direct links to experiment at multiple levels, which at least include cellular and microcircuit, a cycling can be set up and used to help us determine critical mechanisms underlying neurological disease. We illustrate our proposal using our previously developed inhibitory network models in the context of these whole hippocampus preparations and show the importance of having direct links at multiple levels.