bluuebunny/crossref_metadata_2025 huggingface dataset:
Friend, Foe or Frenemy? Traditional Journalism Actors’ Changing Attitudes towards Peripheral Players and Their Innovations
Authors: Chua Sherwin , Duffy Andrew
Published: December 2019

This study synthesises two analytical frameworks—journalistic strangers and agents of media innovation—to examine how perceptions among newsworkers towards new entrants to their field shape the normalisation of innovations in a digital-first legacy news organisation over three years. Based on two rounds of interviews, it finds that peripheral players are gradually recognised for their contributions to journalism by traditional actors. Nonetheless, as barriers between the two groups lower, tensions involving dissonant professional perspectives, practices, and jurisdictions surface and are negotiated. The findings indicate a growing salience of hybrid roles in newsrooms that serve as linchpins to connect divergent professional fields, and more importantly, as bridges between tradition and innovation. Based on the increasing importance of collaboration and hybrid roles, this study makes a theoretical and practical contribution to research and media management by proposing that four forms of proximity—physical, temporal, professional, and control—are crucial in operationalising the impact that peripheral players have on innovation in news organisations.
Negotiating Roles and Routines in Collaborative Investigative Journalism
Authors: Konow-Lund Maria
Published: December 2019

Over the past two decades, the practice of investigative journalism has been reconstructed via the rise of journalistic networks around the world that have layered collaboration atop what had long been an individual pursuit. Among the recent successes of collaborative investigative journalism was the cross-border effort to expose the tax haven leaks that included the Panama Papers (2016). Due to such notable accomplishments, research on cross-border collaboration is increasing, but the ways in which this pooling of resources, time, and networks has impacted practice on a daily basis remain under-investigated. This article looks at how organizations and actors in emerging and legacy newsrooms are negotiating their routines and roles while developing new practices in investigative journalism. It uses three organizations as cases: Bristol Cable, a journalistic co-op operating at the community/local level; the Bureau Local, a local/national data-coordinating news desk; and The Guardian, a legacy media company that has long operated at the national/global level. This article finds that, in the transitions of traditional organizations and journalists and the emergence of new innovative organizations and non-journalistic actors, actors involved in collaborative investigative journalism deploy a language of justification regarding rules between the new and the old. It also finds that concepts such as coordination are part of this negotiation, and that knowledge and knowledge generation are taking place within a traditional understanding of journalism, as the “new” is normalized over time.
Environment for Threat Intelligence Analysis and Generation using Open Sources
Authors: Júnior José Valdy Campelo , Gondim João José Costa
Published: December 2019

Analyzing attacks on computer networks is complex given the volume of data considered and the large number of machines, even in small networks. The volume of data is large and the time to process and analyze it is short. The goal is to extract and analyze information about network attacks that has been obtained from open sources. Using a robust, elastic and scalable architecture that makes use of processing techniques with the use of Hadoop so that the information is available in a timely manner. With the proposed architecture implemented all the desired characteristics were obtained allowing the processing of the data in near real time. The system provides intelligence information about large-scale attacks with agility and efficiency.
Dissipative dynamics within stochastic mean-field approach
Authors: ULGEN İbrahim , YILMAZ Bulent
Published: December 2019

The time-dependent Hartree-Fock (TDHF) and density functional theory (DFT) are among the most useful approaches within mean-field theories for studying static and dynamic properties of complex many-body systems in different branches of physics. Despite the fact that they provide a good approximation for the average properties of one-body degrees of freedoms, they are known to fail to include quantal fluctuations of collective observables and they do not provide sufficient dissipation of collective motion. In order to incorporate these missing effects the stochastic mean-field (SMF) approach was proposed (Ayik 2008). In the SMF approach a set of stochastic initial one-body densities are evolved. Each stochastic one-body density matrix consists of a set of stochastic Gaussian random numbers that satisfy the first and second moments of collective one-body observables. Recent works indicate that the SMF approach provides a good description of the dynamics of the nuclear systems (Yilmaz et al. 2018; Ayik et al. 2019). In this work, the one-dimensional Fermi-Hubbard model is simulated with the SMF approach by using different distributions such as Gaussian, uniform, bimodal and two-point distributions. The dissipative dynamics are discussed and the predictive power of the SMF approach with different probability distributions are compared with each other and the exact dynamics. As a result it is shown that by considering different distributions, the predictive power of the SMF approach can be improved.
Analysis and simulation of single phase-to-ground short circuit fault in Van 154 kV substation: An experimental assessment
Authors: RUSTEMLI Sabir , DEMİR İlter
Published: December 2019

It is necessary to know the short circuit current values of transmission and distribution lines, network equipment, transformers, breakers, fuses etc. to select and install protection systems in an electrical network. It is vital to make this selection correctly for safety, continuous operation, keep supplied power quality in an appropriate level and protect customers from adverse effects of both short circuit currents and outbreaks. In this paper, single phase-to-ground fault short circuit currents which are possible to occur in Van 154 kV substation are modelled under MATLAB / Simulink platform. Simulation results are compared with actual data which is taken from digital relays of substation. Comparison results prove the validation of simulation system.
The concentration effect of Pd and Pt-doped Cu on short range order formation in the rapid cooling process with molecular-dynamics simulation
Authors: ÇELİK Fatih Ahmet , TANBOĞA KORKMAZ Ebru
Published: December 2019

In this study, the formation of bonded pairs which represent amorphous and crystalline structures of Cu-Pd and Cu-Pt ordered alloy systems for different composition of copper was determined with molecular dynamic simulation method during the fast cooling process. The structural properties and phase transformation of systems at different temperatures were investigated with radial distribution functions. The structural formation of bonded pairs was obtained by using Honeycutt-Andersen (HA) method. Physical interactions among atoms were modelled using Sutton-Chen (SC) type of the Embedded Atom Method (EAM) based on many-body interactions. During the rapid cooling process, an amorphous phase conversion was observed at 300K and an increase in the number of bound pairs representing the amorphous phase was detected.
LEGAL WAYS FOR OVERCOMING GENDER DISPROPORTIONS IN THE UKRAINIAN LABOR MARKET
Authors: Sakharuk I.
Published: December 2019

The article deals with the problems of improvement and implementation legislation for gender equality in Ukraine. Have been analysed the main manifestations of discrimination against women in labor market: vertical and horizontal occupational segregation; gender wage differentials; difficulties associated with the combination of work and family responsibilities; the overwhelming predominance of women in the field of part-time, informal, temporary employment; sexual harassment. The issues of gender segregation, gender pay gap, gender equality for work-life balance in the context of foreign and national experience have been explore.

The author have been determine the tendencies of improving the labor legislation in foreign countries for introducing the principle of equality between men and women. Gender mainstreaming strategies and programs in G20 countries were summarized. The author draw attention to the nonefficiency of State Social Program for Equal Rights and Opportunities for Women and Men for the period up to 2021, was made the proposals for its improvement.

The study finds that there is the issue of equality opportunities between men and women in representation authorities at different levels in Ukraine (on the example of the Verkhovna Rada of Ukraine and the Cabinet of Ministers of Ukraine). Approaches to gender quotas in foreign countries have been analyzed. The author used the statistics on employment, entrepreneurship and remuneration to show inequality on the labor martet in Ukraine. The author have also identified that the national legislation, which establishes special guarantees and restrictions on women's work, will have to be improve in the context of a substantive model of equality. Have been suggested the ways of overcoming gender discrimination in the workplace

by a policy of affirmative action
Retinal and optic nerve functional activity after vitrectomy for vitreomacular traction syndrome
Authors: Nikolaenko Evgeniya N. , Kulikov Alexey N. , Volkov Veniamin V. , Danilichev Vladimir F.
Published: December 2019

Background. Impacts of vitrectomy for vitreomacular traction syndrome on retinal and optic nerve functional activity are analyzed.
Materials and methods. The electrophysiological monitoring was carried out before vitrectomy and on Days 1, 3, 7, 14, 30, 60, 180 after surgery in 59 patients (59 eyes). Patients were divided into three groups depending on the intraocular tamponade type: the first group air tamponade, the second group gas (C3F8) tamponade, the third group BSS (balanced salt solution).
Results. A significant inhibition of the functional activity of neurons of internal layers of the retina and of the optic nerve was revealed on the Day 1 after surgery compared to baseline data (р 0.001). In groups I and III, the functional activity of inner layers of the retina and of the optic nerve restored twice as actively as that in the second group.
Conclusions. Vitrectomy causes a reversible and significant inhibition of functional activity of retina and optic nerve. The duration of vitrectomy procedure is a significant negative factor determining the degree of depression of the functional activity of inner layers of the retina and of the optic nerve in the post-op period. The gas tamponade of the vitreous cavity with perfluoropropane-air mixture, compared to air and BSS tamponades, is a significant negative factor influencing the degree of functional activity inhibition of retina and optic nerve after surgery.
Original two-component fibrin glue preparation technique for use in ophthalmic surgery
Authors: Kulikov Alexey N. , Churashov Sergey V. , Kuznetsova Anna Yu. , Gavriluk Ilia O.
Published: December 2019

The study is aimed to modifying the method of a two-component fibrin glue production, as well as studying the possibility of applying glue fixation of the amniotic membrane to the stroma of the cornea, in comparison with interrupted sutures. An experimental study allowed us to determine the optimal composition of the first (base) and second (activating) components, to modify the method for fibrin glue production, and also to test in vivo in six Chinchilla rabbits the fixation of the amniotic membrane using the proposed protocol. The resulting fibrin glue allows not only to securely fix the amniotic membrane to the stroma of the cornea, to reduce the time of the procedure, but also to create conditions for an earlier onset of epithelization while maintaining the transparency of the cornea compared to suture fixation.
Cyclosporine use in “dry eye” syndrome
Authors: Riks Inna A.
Published: December 2019

This literature review focuses on the need for anti-inflammatory therapy for dry eye disease (DED). Causes of development and mechanisms of pathogenesis of DED are presented. Principles of action of various groups of anti-inflammatory medications, as well as recommendations for cyclosporine use of are described. The results of studies on cyclosporine efficacy are highlighted, principles of cyclosporin prescription in DED are listed.
Estimation of High-Frequency Spectral Decay (κ) for the Gyeongju Area in South Korea
Authors: Park Seon Jeong , Lee Jung Mo , Baag Chang-Eob
Published: December 2019

The high-frequency spectral decay parameter kappa (κ) is estimated for the Gyeongju area using records of the temporary seismic array in the Gyeongju area (TSAG), which operated from 2010 to 2013. Gyeongju is a city located in the southeastern part of the Korean Peninsula. Moderate earthquakes frequently occurred around the Gyeongju area in the past; the area has experienced numerous earthquakes for more than a year since an earthquake of ML 5.8 occurred in Gyeongju on 12 September 2016. It is also an important place from an engineering point of view because nuclear facilities are located in the area. The high-frequency spectral decay model of Anderson and Hough (1984) and the standardized procedure for the κ-estimation suggested by Ktenidou et al. (2013) are applied to estimate κ for the Gyeongju area. The estimation gives rise to the equation, κ=0.0219(±0.00151)+7.56×10−5(±1.45×10−5)R indicating that the site-specific κ0-value and the slope κR are 0.0219 s and 7.56×10−5 s/km, respectively, for the Gyeongju area. The κ-estimation for individual stations of TSAG was done to obtain the site-specific κ0-values of stations. Despite the close distance between TSAG stations, the site-specific κ0-values of each station vary. Other than TSAG, κ is also estimated for the permanent borehole station HDB, which has been operated by the Korea Institute of Geoscience and Mineral Resources and is located in the Gyeongju area. The estimated κ0-value of 0.0178 s for HDB is smaller than that of TSAG by 0.0219 s. This is because HDB is a borehole station, but all stations of TSAG are installed at surface. The variation of the κ0-values among the individual TSAG stations and the difference between κ0 for TSAG and that for HDB indicate that spectral decay characteristic at high frequency is affected by surficial sediment layers.
The Influence of Job Satisfaction on Loyalty of Tourism Buses’ Drivers and Their Assistants in Malang through Organizational Commitment
Authors: Nisa' Luthfi Jauharotun
Published: December 2019

A person’s loyalty is a form of loyalty to an object, both people and tasks. But in fact in the current era, loyalty is a difficult thing to find. Human resources who born in 1983-1997 or called Y generation have lower loyalty than X generation (1963-1982). So that studying loyalty of tourism buses’ drivers and their assistants is an interesting thing in order to know what cause the loyalty of drivers and their assistants. The purpose of this study are (1) to find out the description of job satisfaction, organizational commitmen and driver and kernet loyalty, (2) to find out whether there is a significant positive influence on job satisfaction on the driver's and kernet's organizational commitment, (3) to find out whether there is a positive influence significant organizational commitment to driver and kernet loyalty, (4) to determine whether there is a significant positive influence on job satisfaction on driver and kernet loyalty, (5) and to find out whether there is a significant positive effect on job satisfaction on driver loyalty and kernet through organizational commitment. This research is quantitative and using path analysis to determine the relationship between variables. The sampling method uses purposive sampling in the form of judment sampling. Population in this research is 125 driver and kernet in Malang with sample 105 driver and kernet. Data collection methods use a closed questionnaire. The research results show that: (1) Job satisfaction has a positive and significant effect on the driver's and kernet's organizational commitment; (2) Organizational commitment has a positive and significant influence on driver and kernet loyalty; (3) Job satisfaction has a positive and significant impact on driver and kernet loyalty; (4) Job satisfaction affects the loyalty of driver and kernet through organizational commitment.
Pueblos y trabajo indígena en los Andes centrales: Bombón en el siglo XVII
Authors: Quiroz Francisco F.
Published: December 2019

Con base en una documentación rica e inédita, este artículo busca establecer los cambios en las relaciones laborales de los pueblos de indios o reducciones con el surgimiento y consolidación de estancias ganaderas y obrajes privados en las inmediaciones, y la disminución drástica de la población indígena en la meseta de Bombón entre 1667 y 1677 (Andes centrales). Se discuten temas de la historia laboral vigentes en la historiografía peruana y latinoamericana como el trabajo libre y el compulsivo, la mita, el yanaconaje, el peonaje y las reducciones o pueblos de indios. Este estudio encuentra que en las nuevas condiciones los pueblos de indios siguen siendo importantes, pero, con la aparición de mecanismos coercitivos individuales, han dejado de ser imprescindibles para reclutar, retener y remunerar a los trabajadores de las haciendas y el obraje.
Fases, similitudes y diferencias entre los casos de las dictaduras y economía política en Argentina, 1966-1973 y 1976-1983, y Brasil, 1964-1985
Authors: Bona Leandro M. , Páez Sergio M.
Published: December 2019

Argentina y Brasil vivieron dictaduras cívico-militares durante las décadas de 1960 y 1980. En Argentina, la experiencia se dividió en dos etapas: en la primera (1966-1973) se persiguió una estrategia industrializadora; mientras que en la segunda (1976-1983), se alineó al proceso neoliberal. En Brasil, la dictadura (1964-1985) se inició en pleno auge de la etapa desarrollista y no abandonó ese patrón económico. En este artículo se analiza la evolución de ambas experiencias desde la economía política; se indaga en las formas que asumen los procesos de acumulación de capital en cada caso, al analizar cómo las dictaduras incidieron en los mismos, así como los objetivos ideológicos y de política económica que persiguieron. La comparación pone en debate aspectos como las condicionantes del mercado mundial en las dinámicas internas de cada país y las alianzas de clases sociales para entender las similitudes y diferencias entre los dos países.
Educação sexual e universidade: compreensões de graduandos sobre sexualidade e gênero
Authors: Brancaleoni Ana Paula Leivar , De Oliveira Rosemary Rodrigues , Da Silva Caio Samuel Franciscati
Published: December 2019

Buscando reunir elementos no sentido de saber da pertinência, ou não, de propostas de educação sexual a universitários, propôs-se o presente estudo exploratório que tem por objetivo compreender percepções de um grupo de graduandos, de uma faculdade pública, no que se refere a informações sobre sexualidade. Foram aplicados questionários a 32 universitários e constatou-se que: muitos apresentam dúvidas sobre temas relativos à sexualidade; é frequente a prática de sexo sem proteção, resultando em vulnerabilidade quanto a processos de adoecimento e gravidez indesejada; os indivíduos mantêm estereótipos de gênero e mitos sobre a sexualidade; a universidade e os profissionais de saúde são recursos pouco procurados para a obtenção de informações que, na maioria das vezes, são conseguidas através do grupo de pares e familiares. Os dados encontrados apontam para a relevância de se pensar na questão da educação sexual no Ensino Superior de uma forma mais ampla, possibilitando que a universidade seja promotora de reflexão acerca de questões relacionadas à sexualidade e ao gênero possibilitando a mudança de comportamento e o respeito aos diversos.
Teoria do portfólio aplicada na diversificação da produção de café
Authors: Carvalho Gabriel Augusto , Claus Renato Pereira , Mendonça Fabrício Molica , Simão Fúlvio Rodriguez
Published: December 2019

O setor agropecuário representa uma fração significativa do Produto Interno Mineiro, sendo o café um produto historicamente importante no desenvolvimento do Estado. Diante dos riscos incorridos na produção cafeeira tanto por questões climáticas, agronômicas ou de mercado, que impactam a produtividade e rentabilidade obtida, a diversificação com outras culturas pode auxiliar o produtor a minimizar os riscos em seu negócio sendo pelas sinergias entre as culturas ou pela outra fonte de renda. Desse modo, o emprego da teoria dos portfólios para analisar a composição de uma carteira eficiente na produção de café, mostra-se pertinente, pois considera a volatilidade dos retornos dos diferentes produtos para a composição de uma carteira ótima eficiente, que maximize o retorno para dado nível de risco. O objetivo desse estudo é verificar a viabilidade da aplicação e os resultados gerados pela teoria do portfólio de Markowitz (1952) na composição de uma lavoura de café diversificado com outras culturas permanentes. Os resultados obtidos atestam a importância da utilização da teoria do portfólio de Markowitz (1952) para minimizar os riscos e maximizar os retornos diante da existência de ganhos econômicos obtidos coma composição de uma carteira ótima.
Estímulos e Barreiras para a Promoção de uma Cultura da Inovação em uma Empresa Calçadista
Authors: Bessi Vânia Gisele , Oberherr Gláucia , Oliveira Caroline Fernanda de
Published: December 2019

O objetivo desta pesquisa é identificar quais valores organizacionais podem ser caracterizados como estímulos ou barreiras para a inovação, na percepção dos profissionais da área de RH de uma empresa calçadista situada no Vale dos Sinos/RS. Trata-se de uma pesquisa exploratória e descritiva, com abordagem quantitativa, tendo sido realizado um estudo de caso. Para a coleta de dados realizou-se o Inventário de Perfil de Valores Organizacionais – IPVO, proposto por Oliveira e Tamayo (2004), para identificar quais valores organizacionais estão presentes na empresa. Após, foi realizado um segundo questionário, a partir dos resultados do primeiro, para identificar, dentre os valores identificados quais podem ser tidos como estímulos ou barreiras à inovação na organização. Os resultados demonstram que a empresa é familiar e conservadora, e está em fase de reestruturação. Na percepção dos entrevistados, ainda não existe conhecimento claro sobre as mudanças. Por outro lado, em geral, os valores organizacionais presentes na organização foram considerados estímulos à inovação.
Uma análise das práticas de captação de recursos nos programas de pós-graduação stricto sensu públicos em administração
Authors: Ludwig Elton Luiz , Dos Santos Manoela Silveira
Published: December 2019

O objetivo deste estudo foi mapear a captação de recursos nos programas de pós-graduação públicos em Administração. As pesquisas desenvolvidas pelos programas de pós-graduação Stricto Sensu são fundamentais para o desenvolvimento científico e tecnológico do Brasil. Contudo sua manutenção e expansão dependem dos recursos repassados pelo governo federal, que suprimiu parte destes recursos, tornando sua manutenção e busca pela qualidade um grande desafio para os gestores dos programas. Foi possível identificar e analisar como os gestores viabilizam a manutenção dos programas, através do levantamento das práticas de captação de recursos no Stricto Sensu Público em Administração. Para tanto, foi realizado um estudo qualitativo, por meio de entrevistas com os gestores dos programas com nota de avaliação CAPES 5, 6 e 7 no ano de 2017. Os resultados demonstraram a necessidade constante pela busca de recursos, sobretudo na iniciativa privada, baseada nas parcerias firmadas através de cátedras de pesquisa e no aprimoramento da legislação como forma de facilitar a captação de recursos.
“A carne é fraca?” Reação do mercado acionário frente a escândalos corporativos
Authors: Silva Altieres Frances , Penedo Antonio Sérgio Torres , Pereira Vinícius Silva
Published: December 2019

Em março de 2017 o setor brasileiro de carnes e derivados foi surpreendido com a Operação Carne Fraca, que investigou denúncias de adulteração nos alimentos produzidos por empresas desse segmento. Dentre as investigadas estavam a BRF e a JBS, as duas maiores companhias brasileiras do ramo. Assim, este artigo objetivou averiguar o impacto dessa operação no valor das empresas do setor de carne e derivados listadas na B3. Testou-se a hipótese de eficiência do mercado na forma semiforte e identificou-se os possíveis impactos para acionistas na comparação entre o período anterior e posterior à operação. Aplicou-se o método do estudo de eventos, obtendo-se os dados por meio do site da B3 e pela base Economatica. Rejeitou-se a hipótese nula de não eficiência do mercado (H0A), visto que os retornos anormais acumulados na data do evento foram estatisticamente diferentes de zero. Na comparação dos 20 dias anteriores e posteriores à janela do evento, o teste t pareado demonstrou que a operação não impactou o mercado mesmo após a janela de cinco dias, não se rejeitando a hipótese nula H0B. Sugeriu-se para pesquisas futuras o aumento na janela de estimativa do modelo de regressão e que se teste a hipótese de mercados adaptativos.
Personagens nas músicas de Raul Seixas: possibilidades educacionais
Authors: Duarte André Luís Faria
Published: December 2019

O processo educacional tem sido questionado atualmente, em grande parte devido ao pouco interesse despertado no aluno e ao modelo de educação focada na transmissão de conteúdo. Desta forma, com o intuído de contribuir com uma educação mais rica a partir do diálogo com expressões culturais, o presente estudo busca identificar as possibilidades de utilização das músicas de Raul Seixas em situações de aprendizagem, tendo como referência os diversos personagens que aparecem nas letras das músicas desse compositor. O presente estudo faz uma análise de conteúdo das letras das músicas, identificando a ocorrência de personagens históricos, religiosos, fictícios e do mundo das artes, levando em conta o contexto em que são inseridos, com objetivo de identificar a possibilidade de usos na educação. Como resultado, percebe-se que a variedade de personagens não é a principal característica de suas músicas quando se pensa em utilizá-las na educação, que poderia ser mais rico ao se considerar todo o contexto social e histórico nos quais o artista esteve inserido.
Stan lee, o criador de tendências: super-heróis e a cultura jovem nos anos 1960
Authors: Miorando Guilherme Sfredo
Published: December 2019

Este ensaio explora os jogos de mercado presentes nas estratégias de comunicação de Stan Lee e da Marvel. Também exporemos como Stan se aproveitou da cultura da juventude em ebulição nos anos 1960 para criar tendências e se beneficiar das modas que estavam surgindo. Enquanto as agências de publicidade se debatiam para tentar entender o público jovem, que queria romper tanto com a infância quanto com os adultos quadrados, Stan Lee enchia plateias de universidades e era aplaudido por estudantes. Tentaremos explicar como o carisma de Stan Lee o aproximou de um status de líder visionário, capaz de manipular símbolos e mitos modernos e transformá-los numa espécie de culto, através das narrativas super-heróicas. Buscaremos elucidar como sua existência ajudou a consolidar uma comunidade de fãs e expandi-la para o enorme sucesso de bilheterias de cinema que suas criações se tornaram e que persistem para além de sua morte, no ano de 2018
Stan Lee, o Quarteto Fantástico e a evolução da divulgação científica nas histórias em quadrinhos de super-heróis: possibilidades para uma aula de ciências
Authors: Nascimento Francisco
Published: December 2019

Apresentamos uma análise da evolução da divulgação científica nas histórias em quadrinhos de super-heróis, com foco na origem do Quarteto Fantastico (criado por Stan Lee em 1962) e sua repaginação para a primeira década do século XXI, o chamado “Quarteto Fantástico Ultimate”. A possibilidade de uso da leitura deste material em aulas de Ciências é abordada a partir do trabalho de Zanetic (1989), com o objetivo de analisar seu potencial de uso como ferramenta capaz de apresentar aos alunos uma visão diferenciada dos conceitos científicos.
A distopia provocada. Uma leitura sobre a formação docente e a atenção à escolarização de pessoas com deficiência
Authors: Silva Shirley , Zago Cesar Claudinei
Published: December 2019

O presente artigo tem como intencionalidade apresentar o cotejamento realizado entre as diretrizes curriculares para a formação docente e as diretrizes de operacionalização de atenção direcionada às deficiências. Considerando que nas diretrizes operacionais para o atendimento educacional especializado instituem-se, em seu corpus, o papel e a função do docente que atuará nesse atendimento, consequentemente implicam em uma proposta de formação para tal. Consideramos, ainda, que o cotejamento, imperioso nas reflexões que buscam problematizar o cenário sobre a qual a formação se materializa nesse campo, permitiu aproximar, por meio dos dispositivos teórico-discursivos de tais documentos, a uma situação que denominamos de nexo-desconexo, pois tanto não se compatibilizam em termos conceituais, como em termos da relação entre as diretrizes da formação com a ação docente, propriamente dita. Visando não reiterar o embaçamento que a utilização de determinados enunciados provoca, adotamos como referência para a discussão “direito à escolarização, atenção direcionada às deficiências e roteiros”. A partir dessa perspectiva, o texto apresenta incialmente como esse nexo-desconexo se insere em uma discussão mais ampliada daquela que responsabiliza a escola e a docência pela superação das desigualdades, potencializada quando se refere às deficiências, o que vem imprimindo à docência a figura da onipotência. Tomando a análise do discurso crítica apresentamos a análise propriamente dos documentos oficiais, posteriormente a um breve panorama da amplitude da discussão acadêmica sobre a formação docente, reiterando a complexidade da temática. Essa discussão nos leva a afirmar que da docência tem sido esperado um papel de onipotência, ainda, que o nexo-desconexo permite aludir a uma situação de distopia, especialmente quando pensamos em uma formação que tenha superado a dicotomia o “nós” e o “eles” ou o “comum” e o “especial”.
Projetos de valorização da cultura e da religiosidade nas escolas: um olhar sobre o projeto avença da banda placa
Authors: Videira Piedade Lino , Custódio Elivaldo Serrão , Pereira Elisângela De Souza , Araujo Jéssica Baia
Published: December 2019

O presente artigo tem por objetivo analisar as potencialidades didático-pedagógicas existentes no conteúdo histórico, cultural, sociológico, étnico-racial e educacional presente no Projeto Avença da Banda Placa da cidade de Macapá-AP. Assim, a pesquisa teve por objetivo ainda verificar sua contribuição para o processo de salvaguarda, reconhecimento, valorização e promoção do patrimônio cultural afro-amapaense, bem como a compreensão do papel da cultura na relação ensino-aprendizagem escolar por meio de projetos interdisciplinares Trata-se de uma pesquisa de natureza qualitativa descritiva cuja coleta de dados foi a pesquisa bibliográfica, documental, o questionário e a entrevista semiestruturada como forma de investigação. Os sujeitos da pesquisa foram os próprios integrantes da Banda Placa. Assim, os resultados obtidos neste trabalho confirmam que a iniciativa da Banda Placa de salvaguardar o patrimônio cultural afro-amapaense, através do Projeto Avença, é uma forma de promover conhecimento sobre a cultura local, numa perspectiva interdisciplinar, além de ajudar na construção da identidade cultural do povo do Amapá em geral.
O conservadorismo e a formação cidadã: a abordagem da Sexualidade no Ensino Fundamental diante do discurso em documentos oficiais
Authors: Moura Francisco Nunes de Sousa , Leite Raquel Crosara Maia
Published: December 2019

Apesar da sexualidade pertencer ao desenvolvimento humano, desde a sua formação até o seu envelhecimento, ela ainda é considerada como um tabu dentro da sociedade e tema de constrangimento para o diálogo entre as pessoas. Assim, torna-se importante instigar o debate deste tema nos mais diversos ambientes da sociedade e, sobretudo, na educação escolar, a qual contribui na preparação de indivíduos para sua inserção na sociedade. Entretanto, essas indicações são efetivadas ao adicionar tal temática em propostas governamentais para a educação, no intuito de estimular a abordagem da sexualidade no currículo das instituições de ensino. Desta forma, esta pesquisa objetiva analisar como a temática sexualidade tem sido abordada nos Parâmetros Curriculares Nacionais (PCN) e na Base Nacional Comum Curricular (BNCC), ambos para o Ensino Fundamental, diferenciando a abordagem da sexualidade na educação. Para efetivação desta pesquisa qualitativa, realizou-se uma análise documental nos PCN e na BNCC - documentos vigorados em 1997 e 2017, respectivamente, buscando identificar suas propostas para a abordagem da temática sexualidade. Averiguou-se a construção de um volume específico dos PCN para o ensino de sexualidade, intitulado volume 10 – Pluralidade Cultural e Orientação Sexual. O material indica a inserção da temática em modelo transversal, ou seja, ao decorrer de todo o Ensino Fundamental, a perpassar distintas disciplinas curriculares. A BNCC, por sua vez, propõe a inclusão da temática apenas para a disciplina de Ciências, no 8º ano. Neste sentido, considera-se importante repensar sobre as formas de apresentação do tema sexualidade na Educação Básica, visto o retrocesso da abordagem na BNCC, limitando a uma disciplina e a um ano escolar específico.
Structure and dynamics of a DNA duplex containing single alpha-anomeric deoxyadenosine residue.
Authors: Bielecki L , Adamiak R W
Published: December 2019

Structure and dynamics of an undecamer DNA duplex containing a single alpha-anomeric deoxyadenosine residue placed in opposition to a thymidine unit have been studied using simulation of molecular dynamics in aqueous solution. Despite several noticeable deviations from the B-DNA duplex structure caused by the anomerisation, such as: West type puckering of the alpha-anomeric sugar, disrupted base stacking pattern and unstable duplex bending, the formation of a non-classical alpha-dA-T pair was observed. A novel way of visual presentation of trajectory data allowing high throughput screening of the conformational parameters is presented.
Cation binding properties of calretinin, an EF-hand calcium-binding protein.
Authors: Groves P , Palczewska M
Published: December 2019

Calretinin (CR) is a neuronal EF-hand protein previously characterized as a calcium (micromolar affinity) binding protein. CR-containing neurons are spared in some neurodegenerative diseases, although it is as yet unconfirmed how CR plays an active role in this protection. Higher levels of some metal cations (e.g. copper and zinc) are associated with these diseases. At the same time, metals such as terbium (NMR and fluorescence) cadmium (NMR) and manganese (EPR) serve as useful calcium analogues in the study of EF-hand proteins. We survey the binding of the above-mentioned metal cations that might affect the structure and function of CR. Competitive 45Ca2+-overlay, competitive terbium fluorescence and intrinsic tryptophan fluorescence are used to detect the binding of metal cations to CR. Terbium and copper (half-maximal effect of 15 microM) bind to CR. Terbium has a similar or greater affinity for the calcium-binding sites of CR than calcium. Copper quenches the fluorescence of terbium-bound CR, and CR tryptophan residues and competes weakly for 45Ca2+-binding sites. Cadmium, magnesium, manganese and zinc bind less strongly (half-maximal effects above 0.1 mM). Therefore, only terbium appears to be a suitable analytical calcium analogue in further studies of CR. The principal conclusion of this work is that copper, in addition to calcium, might be a factor in the function of CR and a link between CR and neurodegenerative diseases.
Mitochondrial uncoupling does not influence the stability of the intracellular signal activating plasma membrane calcium channels.
Authors: Zabłocki K , Makowska A , Duszyński J
Published: December 2019

The effects of various concentrations of thapsigargin, a specific inhibitor of Ca2+-ATPase in the endoplasmic reticulum (ER) membrane, on calcium homeostasis in lymphoidal T cells (Jurkat) were investigated. Preincubation of these cells suspended in nominally calcium-free medium with 0.1 microM thapsigargin resulted in a complete release of Ca2+ from intracellular calcium stores. When the medium was supplemented with 3 mM CaCl2 the cells maintained constantly elevated level of cytosolic Ca2+. However, thapsigargin applied at lower concentration produced only a partial depletion of the stores. For example, in the cells pretreated with 1 nM thapsigargin and suspended in calcium-free medium approximately 75% of the calcium content was released from the intracellular stores. The addition of 3 mM CaCl2 to such cell suspension led to a transient increase in cytosolic calcium concentration, followed by a return to a lower steady-state. This phenomenon, related to the refilling of the ER by Ca2+, allowed to estimate the half-time for the process of cell recovery after activation of store-operated calcium channels. By this approach we have found that carbonyl cyanide m-chlorophenylhydrazone, which has been documented to inhibit calcium entry into Jurkat cells, does not influence the stability of the intracellular signal involved in the activation of store-operated calcium channels.
Signal transduction in confluent C3H 10T1/2 cells. The role of focal adhesion kinase.
Authors: Miłoszewska J , Trembacz H , Janik P
Published: December 2019

The activities of extracellular signal-regulated kinases (ERK1/ERK2) is required for proliferation of several types of cells. The performed analysis showed stimulation of ERK's by fetal calf serum (FCS) or fibronectin in the C3H 10T1/2 cell cultures at logarithmic phase of growth. The ERKs activity was not stimulated in confluent cells. This could not be accounted for a partial down regulation of ERK since its level was stable in both types of cells regardless of their density and kind of stimulation. Searching for ERK up-stream elements we studied the integrin receptor gene transcript by RT-PCR and focal adhesion kinase (FAK) by Western blotting and phosphorylation assays. It was found that FCS and fibronectin stimulated phosphorylating activity of FAK in the cells at the logarithmic phase of growth, but were inefficient in the confluent cells. RT-PCR showed the presence of alpha5 and beta1 integrin transcripts, and p125FAK was at the same level regardless of the type of stimulation. These data indicate that the ability of FAK to be activated plays an important role in ERK regulation and, in consequence in proliferation and growth inhibition during confluence.
The flavonoids, quercetin and isorhamnetin 3-O-acylglucosides diminish neutrophil oxidative metabolism and lipid peroxidation.
Authors: Zielińska M , Kostrzewa A , Ignatowicz E , Budzianowski J
Published: December 2019

Two natural flavonoids, quercetin and isorhamnetin 3-O-acylglucosides, were examined for their inhibitory influence on the in vitro production and release of reactive oxygen species in polymorphonuclear neutrophils (PMNs). The generation of superoxide radical, hydrogen peroxide and hypochlorous acid were measured by, respectively, cytochrome c reduction, dichlorofluorescin oxidation and taurine chlorination. Membrane lipid oxidation was studied by the thiobarbituric acid method in mouse spleen microsomes. The addition of flavonoids at the concentration range 1-100 microM inhibited PMNs oxidative metabolism and lipid peroxidation in a dose-dependent manner. The results suggest that these flavonoids suppress the oxidative burst of PMNs and protect membranes against lipid peroxidation.
Modulation of human deoxycytidine kinase activity as a response to cellular stress induced by NaF.
Authors: Csapó Z , Sasvári-Székely M , Spasokoukotskaja T , Staub M
Published: December 2019

Deoxycytidine kinase (dCK) is one of the key enzymes of deoxynucleoside salvage supplying resting lymphocytes with DNA precursors for synthesis and repair. The level of dCK activity is especially important in chemotherapy with the use of deoxynucleoside analogues like arabinosyl cytosine (Citarabid, ara-C), or 2-chloro-deoxyadenosine (Cladribine, CdA). Previous results showed that Cladribine treatment of human lymphocytes increased several fold the activity of dCK without increasing the amount of dCK protein itself (Sasvári-Székely, et al., 1998, Biochem. Pharmacol. 56, 1175), and a possible post-translational modification was suggested. This theory was further investigated using NaF as an inhibitor of protein phosphatases. It was shown that NaF treatment of cells elevated dCK activity while inhibiting DNA synthesis. The possible mechanism of dCK activation/inactivation induced by exposure of cell cultures to different agents is discussed.
Chlorination of N-acetyltyrosine with HOCl, chloramines, and myeloperoxidase-hydrogen peroxide-chloride system.
Authors: Drabik G , Naskalski J W
Published: December 2019

N-acetyl-L-tyrosine (N-acTyr), with the alpha amine residue blocked by acetylation, can mimic the reactivity of exposed tyrosyl residues incorporated into polypeptides. In this study chlorination of N-acTyr residue at positions 3 and 5 in reactions with NaOCl, chloramines and the myeloperoxidase (MPO)-H2O2-Cl- chlorinating system were invesigated. The reaction of N-acTyr with HOCl/OCl- depends on the reactant concentration ratio employed. At the OCl-/N-acTyr (molar) ratio 1:4 and pH 5.0 the chlorination reaction yield is about 96% and 3-chlorotyrosine is the predominant reaction product. At the OCl-/N-acTyr molar ratio 1:1.1 both 3-chlorotyrosine and 3,5-dichlorotyrosine are formed. The yield of tyrosine chlorination depends also on pH, amounting to 100% at pH 5.5, 91% at pH 4.5 and 66% at pH 3.0. Replacing HOCl/OCl- by leucine/chloramine or alanine/chloramine in the reaction system, at pH 4.5 and 7.4, produces trace amount of 3-chlorotyrosine with the reaction yield of about 2% only. Employing the MPO-H2O2-Cl- chlorinating system at pH 5.4, production of a small amount of N-acTyr 3-chloroderivative was observed, but the reaction yield was low due to the rapid inactivation of MPO in the reaction system. The study results indicate that direct chlorination of tyrosyl residues which are not incorporated into the polypeptide structure occurs with excess HOCl/OCl- in acidic media. Due to the inability of the myeloperoxidase-H2O2-Cl- system to produce high enough HOCl concentrations, the MPO-mediated tyrosyl residue chlorination is not effective. Semistable amino-acid chloramines also appeared not effective as chlorine donors in direct tyrosyl chlorination.
IMPACTOS AMBIENTAIS DO MANEJO FLORESTAL COMUNITÁRIO NA FLORESTA NACIONAL DO TAPAJÓS
Authors: Carvalho Adenomar Neves de , Schultz de Carvalho Telma Lélia Gonçalves , Gama João Ricardo Vasconcelos
Published: December 2019

Este trabalho teve o objetivo de avaliar os impactos ambientais decorrentes do manejo florestal comunitário na Floresta Nacional do Tapajós executado pela Cooperativa Mista da FLONA do Tapajós (COOMFLONA) analisando aspectos intrínsecos ao meio físico, biológico e socioeconômico, especialmente os efeitos locais diretos e indiretos. Foi realizada a identificação e a caracterização dos impactos com dados qualitativos baseados na magnitude e importância dos mesmos sobre o meio e disposto numa matriz de Leopoldo. Na avaliação verificaram-se as ações que geraram alterações nos hábitos da população tradicional, compactação do solo, redução de habitats, aumento da atividade de caça, danos às árvores remanescentes, abertura de ramais, clareiras e pátios de estocagem, as quais geraram o afugentamento e extinção local da fauna temporariamente, aumento dos processos erosivos e diminuição da base genética vegetal. Embora o manejo florestal adotado pelos comunitários impacte menos que a exploração convencional, este ainda afeta a floresta, porém, não rompe a sua resiliência e, de certo modo, promove a conservação ambiental.                                                              PALAVRAS-CHAVE: Sistema de produção, Conservação, Amazônia.
SANITARY-TECHNOLOGICAL EVENTS OF BREAD PRODUCTION WITHOUT YEAST
Authors: Magomedov Gazibeg O. , Plotnikova I. V. , Magomedov M. G. , Cheshinsky V. L.
Published: December 2019

A method of bread production without using baker’s yeast by using whole-ground wheat flour according to fundamentally new technology through the mechanical loosening of dough was developed. To produce the product, the work was carried out in three stages: at the first stage - the quality of grain was studied at separate stages of its preparation for grinding; on the second - the quality of whole-grain flour obtained by the disintegrating-wave method with a weak microwave information effect was analyzed; in the third - the quality of bread was assessed. Thorough cleaning of the grain and it’s repeated passing through scouring machines, grinding by the wave-disintegrating method with the energy of the electromagnetic field of ultra-high frequency has been proven to provide effective disinfection of grain from various pollutants and reduce the microbiological seeding of whole flour and bread obtained by a mechanical method of dough loosening. To improve the taste characteristics, nutritional value and microbiological purity of bread the concentrated apple juice was added to the bread’s recipe. The obtained indices of microbiological contamination of the cooked bread, which packed in a transparent polypropylene film “BIAXPLEN”, after 7 days of storage, show that in the bread from the whole-grain wheat flour the contamination is lower by 32.6% compared to the bread from entire wheat flour. Improving the microbiological purity of whole-wheat flour became possible by using traditional methods of grain preparing for milling, using the wave-disintegrating method of grinding grain into flour by disinfecting the product with electromagnetic energy of ultra-high frequency, adding concentrated apple juice to the bread’s recipe. Compared with the traditional bread made from wheat flour, the developed bread has increased nutritional value and reduced calorie content.