bluuebunny/crossref_metadata_2025 huggingface dataset:
Features Importance Analysis of Diesel Vehicles’ NOx and CO2 Emission Predictions in Real Road Driving Based on Gradient Boosting Regression Model
Authors: Wen Hung-Ta , Lu Jau-Huai , Jhang Deng-Siang
Published: December 2021

In order to have an accurate and fast prediction of the artificial intelligence (AI) model, the choice of input features is at least as important as the choice of model. The effect of input features selection on the emission models of light diesel vehicles driven on real roads was investigated in this paper. The gradient boosting regression (GBR) model was used to train and to predict the emissions of nitrogen oxide (NOx), carbon dioxide (CO2), and the fuel consumption of real driving diesel vehicles in urban scenarios, the suburbs, and on highways. A portable emissions measurement system (PEMS) system was used to collect data of vehicles as well as environmental conditions. The vehicle was run on two routes. The model was trained with the first route data and was used to predict the emissions of the second route. There were ten features related to the NOx model and nine features associated with the CO2 model. The importance of each feature was sorted, and a different number of features were used as input to train the models. The best NOx model had the coefficient of determination (R2) values of 0.99, 0.99, and 0.99 in each driving pattern (urban, suburbs, and highways). Predictions of the second route had the R2 values of 0.88, 0.89, and 0.96 respectively. The best CO2 model had the R2 values of 0.98, 0.99, and 0.99 in each driving pattern, respectively. Predictions of the second route had the R2 values are 0.79, 0.82, and 0.83, respectively. The most important features for the NOx model are mass air flow rate (g/s), exhaust flow rate (m3/min), and CO2 (ppm), while the important features for the CO2 model are exhaust flow rate (m3/min) and mass air flow rate (g/s). It is noted that the regression models based on the top three features may give predictions very close to the measured data.
Autism-Related Transcription Factors Underlying the Sex-Specific Effects of Prenatal Bisphenol A Exposure on Transcriptome-Interactome Profiles in the Offspring Prefrontal Cortex
Authors: Kanlayaprasit Songphon , Thongkorn Surangrat , Panjabud Pawinee , Jindatip Depicha , Hu Valerie W. , Kikkawa Takako , Osumi Noriko , Sarachana Tewarit
Published: December 2021

Bisphenol A (BPA) is an environmental risk factor for autism spectrum disorder (ASD). BPA exposure dysregulates ASD-related genes in the hippocampus and neurological functions of offspring. However, whether prenatal BPA exposure has an impact on genes in the prefrontal cortex, another brain region highly implicated in ASD, and through what mechanisms have not been investigated. Here, we demonstrated that prenatal BPA exposure disrupts the transcriptome–interactome profiles of the prefrontal cortex of neonatal rats. Interestingly, the list of BPA-responsive genes was significantly enriched with known ASD candidate genes, as well as genes that were dysregulated in the postmortem brain tissues of ASD cases from multiple independent studies. Moreover, several differentially expressed genes in the offspring’s prefrontal cortex were the targets of ASD-related transcription factors, including AR, ESR1, and RORA. The hypergeometric distribution analysis revealed that BPA may regulate the expression of such genes through these transcription factors in a sex-dependent manner. The molecular docking analysis of BPA and ASD-related transcription factors revealed novel potential targets of BPA, including RORA, SOX5, TCF4, and YY1. Our findings indicated that prenatal BPA exposure disrupts ASD-related genes in the offspring’s prefrontal cortex and may increase the risk of ASD through sex-dependent molecular mechanisms, which should be investigated further.
Increased Expression of Flightless I in Cutaneous Squamous Cell Carcinoma Affects Wnt/β-Catenin Signaling Pathway
Authors: Yang Gink N. , Strudwick Xanthe L. , Bonder Claudine S. , Kopecki Zlatko , Cowin Allison J.
Published: December 2021

Cutaneous squamous cell carcinoma (cSCC) accounts for 25% of cutaneous malignancies diagnosed in Caucasian populations. Surgical removal in combination with radiation and chemotherapy are effective treatments for cSCC. Nevertheless, the aggressive metastatic forms of cSCC still have a relatively poor patient outcome. Studies have linked actin cytoskeletal dynamics and the Wnt/β-catenin signaling pathway as important modulators of cSCC pathogenesis. Previous studies have also shown that the actin-remodeling protein Flightless (Flii) is a negative regulator of cSCC. The aim of this study was to investigate if the functional effects of Flii on cSCC involve the Wnt/β-catenin signaling pathway. Flii knockdown was performed using siRNA in a human late stage aggressive metastatic cSCC cell line (MET-1) alongside analysis of Flii genetic murine models of 3-methylcholanthrene induced cSCC. Flii was increased in a MET-1 cSCC cell line and reducing Flii expression led to fewer PCNA positive cells and a concomitant reduction in cellular proliferation and symmetrical division. Knockdown of Flii led to decreased β-catenin and a decrease in the expression of the downstream effector of β-catenin signaling protein SOX9. 3-Methylcholanthrene (MCA)-induced cSCC in Flii overexpressing mice showed increased markers of cancer metastasis including talin and keratin-14 and a significant increase in SOX9 alongside a reduction in Flii associated protein (Flap-1). Taken together, this study demonstrates a role for Flii in regulating proteins involved in cSCC proliferation and tumor progression and suggests a potential role for Flii in aggressive metastatic cSCC.
Polar Interactions at the Dimer–Dimer Interface of Methionine Adenosyltransferase MAT I Control Tetramerization
Authors: Sánchez-Pérez Gabino Francisco , Pajares María Ángeles
Published: December 2021

Catalytic MATα1 subunits associate into kinetically distinct homo-dimers (MAT III) and homo-tetramers (MAT I) that synthesize S-adenosylmethionine in the adult liver. Pathological reductions in S-adenosylmethionine levels correlate with MAT III accumulation; thus, it is important to know the determinants of dimer–dimer associations. Here, polar interactions (<3.5 Å) at the rat MAT I dimer–dimer interface were disrupted by site-directed mutagenesis. Heterologous expression rendered decreased soluble mutant MATα1 levels that appeared mostly as dimers. Substitutions at the B1–B2 or B3–C1 β-strand loops, or changes in charge on helix α2 located behind, induced either MAT III or MAT I accumulation. Notably, double mutants combining neutral changes on helix α2 with substitutions at either β-strand loop further increased MAT III content. Mutations had negligible impact on secondary or tertiary protein structure, but induced changes of 5–10 °C in thermal stability. All mutants preserved tripolyphosphatase activity, although AdoMet synthesis was only detected in single mutants. Kinetic parameters were altered in all purified proteins, their AdoMet synthesis Vmax and methionine affinities correlating with the association state induced by the corresponding mutations. In conclusion, polar interactions control MATα1 tetramerization and kinetics, diverse effects being induced by changes on opposite β-sheet loops putatively leading to subtle variations in central domain β-sheet orientation.
Salt-Sensitive Hypertension in GR+/− Rats Is Accompanied with Dysregulation in Adrenal Soluble Epoxide Hydrolase and Polyunsaturated Fatty Acid Pathways
Authors: Vanderriele Paul-Emmanuel , Wang Qing , Mérillat Anne-Marie , Ino Frédérique , Aeschlimann Gilles , Ehret Xavier , Ancin Del Olmo David , Ponce de León Verónica , Scholl Ute I. , Winter Denise V. , Odermatt Alex , Hummler Edith , Verouti Sophia N.
Published: December 2021

Mutations within the glucocorticoid receptor (GR) gene locus lead to glucocorticoid resistance which is characterized by several clinical symptoms such as adrenal gland hyperplasia and salt-sensitive hypertension, although the underlying mechanisms are still unknown. We studied GR haploinsufficient (GR+/−) Sprague Dawley rats which, on a standard diet, showed significantly increased plasma aldosterone and corticosterone levels and an adrenocortex hyperplasia accompanied by a normal systolic blood pressure. Following a high salt diet, these rats developed salt-sensitive hypertension and maintained elevated enzyme-soluble epoxide hydrolase (sEH) in adrenal glands, while sEH was significantly decreased in wild-type rats. Furthermore, GR+/− rats showed dysregulation of the equilibrated linoleic and arachidonic acid pathways, with a significant increase of less active metabolites such as 8,9-DiHETrE. In Sprague Dawley rats, GR haploinsufficiency induced steroid disturbances, which provoked hypertension only in combination with high salt intake, which was accompanied by disturbances in sEH and fatty acid metabolism. Our results suggest that sEH inhibition could be a potential target to treat hypertension in patients with GR haploinsufficiency.
Effects and Related Mechanisms of the Senolytic Agent ABT-263 on the Survival of Irradiated A549 and Ca9-22 Cancer Cells
Authors: Sato Kota , Iwasaki Soichiro , Yoshino Hironori
Published: December 2021

Senolytic agents eliminate senescent cells and are expected to reduce senescent cell-mediated adverse effects in cancer therapy. However, the effects of senolytic agents on the survival of irradiated cancer cells remain unknown. Here, the effects of the senolytic agent ABT-263 on the survival of irradiated A549 and Ca9-22 cancer cells were investigated. ABT-263 was added to the culture medium after irradiation. SA-β-gal activity and cell size, which are hallmarks of cell senescence, were evaluated using a flow cytometer. The colony-forming assay and annexin V staining were performed to test cell survival. We first confirmed that radiation increased the proportion of cells with high SA-β-gal activity and that ABT-263 decreased it. Of note, ABT-263 decreased the survival of irradiated cancer cells and increased the proportion of radiation-induced annexin V+ cells. Furthermore, the caspase inhibitor suppressed the ABT-263-induced decrease in the survival of irradiated cells. Intriguingly, ABT-263 decreased the proportion of SA-β-gal low-activity/large cells in the irradiated A549 cells, which was recovered by the caspase inhibitor. Together, these findings suggest that populations maintaining the ability to proliferate existed among the irradiated cancer cells showing senescence-related features and that ABT-263 eliminated the population, which led to decreased survival of irradiated cancer cells.
The Influence of Prenatal Fumonisin Exposure on Bone Properties, as well as OPG and RANKL Expression and Immunolocalization, in Newborn Offspring Is Sex and Dose Dependent
Authors: Tomaszewska Ewa , Rudyk Halyna , Świetlicka Izabela , Hułas-Stasiak Monika , Donaldson Janine , Arczewska Marta , Muszyński Siemowit , Dobrowolski Piotr , Puzio Iwona , Kushnir Volodymyr , Brezvyn Oksana , Muzyka Viktor , Kotsyumbas Ihor
Published: December 2021

The current study examined the effects of exposure of pregnant dams to fumonisins (FBs; FB1 and FB2), from the seventh day of pregnancy to parturition, on offspring bone metabolism and properties. The rats were randomly divided into three groups intoxicated with FBs at either 0, 60, or 90 mg/kg b.w. Body weight and bone length were affected by fumonisin exposure, irrespective of sex or dose, while the negative and harmful effects of maternal FBs’ exposure on bone mechanical resistance were sex and dose dependent. The immunolocalization of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-Β ligand (RANKL), in bone and articular cartilage, indicated that the observed bone effects resulted from the FB-induced alterations in bone metabolism, which were confirmed by the changes observed in the Western blot expression of OPG and RANKL. It was concluded that the negative effects of prenatal FB exposure on the general growth and morphometry of the offspring bones, as a result of the altered expression of proteins responsible for bone metabolism, were dose and sex dependent.
Psychological Stress, Mast Cells, and Psoriasis—Is There Any Relationship?
Authors: Woźniak Ewelina , Owczarczyk-Saczonek Agnieszka , Placek Waldemar
Published: December 2021

Psoriasis vulgaris is a common inflammatory skin disease with still unknown pathogenesis. In recent years, genetic and environmental factors have been mentioned as the main causes. Among environmental factors, many researchers are trying to investigate the role of mental health and its importance in the development of many diseases. In the pathophysiology of psoriasis, the role of the interaction between the nervous, endocrine, and immune systems are often emphasized. So far, no one has clearly indicated where the pathological process begins. One of the hypotheses is that chronic stress influences the formation of hormonal changes (lowering the systemic cortisol level), which favors the processes of autoimmunity. In inflammatory skin conditions, mast cells (MCs) are localized close to blood vessels and peripheral nerves, where they probably play an important role in the response to environmental stimuli and emotional stress. They are usually connected with a fast immune response, not only in allergies but also a protective response to microbial antigens. Among many cells of the immune system, MCs have receptors for the hormones of the hypothalamic–pituitary–adrenal (HPA) axis on their surface. In this review, we will try to take a closer look at the role of MCs in the pathophysiology of psoriasis. This knowledge may give the opportunity to search for therapeutic solutions.
The Stimulatory Effects of Intracellular α-Synuclein on Synaptic Transmission Are Attenuated by 2-Octahydroisoquinolin-2(1H)-ylethanamine
Authors: Ramirez Alejandra E. , Fernández-Pérez Eduardo J. , Olivos Nicol , Burgos Carlos F. , Boopathi Subramanian , Armijo-Weingart Lorena , Pacheco Carla R. , González Wendy , Aguayo Luis G.
Published: December 2021

α-Synuclein (αSyn) species can be detected in synaptic boutons, where they play a crucial role in the pathogenesis of Parkinson’s Disease (PD). However, the effects of intracellular αSyn species on synaptic transmission have not been thoroughly studied. Here, using patch-clamp recordings in hippocampal neurons, we report that αSyn oligomers (αSynO), intracellularly delivered through the patch electrode, produced a fast and potent effect on synaptic transmission, causing a substantial increase in the frequency, amplitude and transferred charge of spontaneous synaptic currents. We also found an increase in the frequency of miniature synaptic currents, suggesting an effect located at the presynaptic site of the synapsis. Furthermore, our in silico approximation using docking analysis and molecular dynamics simulations showed an interaction between a previously described small anti-amyloid beta (Aβ) molecule, termed M30 (2-octahydroisoquinolin-2(1H)-ylethanamine), with a central hydrophobic region of αSyn. In line with this finding, our empirical data aimed to obtain oligomerization states with thioflavin T (ThT) and Western blot (WB) indicated that M30 interfered with αSyn aggregation and decreased the formation of higher-molecular-weight species. Furthermore, the effect of αSynO on synaptic physiology was also antagonized by M30, resulting in a decrease in the frequency, amplitude, and charge transferred of synaptic currents. Overall, the present results show an excitatory effect of intracellular αSyn low molecular-weight species, not previously described, that are able to affect synaptic transmission, and the potential of a small neuroactive molecule to interfere with the aggregation process and the synaptic effect of αSyn, suggesting that M30 could be a potential therapeutic strategy for synucleinopathies.
Antifungal Peptides and Proteins to Control Toxigenic Fungi and Mycotoxin Biosynthesis
Authors: Martínez-Culebras Pedro V. , Gandía Mónica , Garrigues Sandra , Marcos Jose F. , Manzanares Paloma
Published: December 2021

The global challenge to prevent fungal spoilage and mycotoxin contamination on food and feed requires the development of new antifungal strategies. Antimicrobial peptides and proteins (AMPs) with antifungal activity are gaining much interest as natural antifungal compounds due to their properties such as structure diversity and function, antifungal spectrum, mechanism of action, high stability and the availability of biotechnological production methods. Given their multistep mode of action, the development of fungal resistance to AMPs is presumed to be slow or delayed compared to conventional fungicides. Interestingly, AMPs also accomplish important biological functions other than antifungal activity, including anti-mycotoxin biosynthesis activity, which opens novel aspects for their future use in agriculture and food industry to fight mycotoxin contamination. AMPs can reach intracellular targets and exert their activity by mechanisms other than membrane permeabilization. The mechanisms through which AMPs affect mycotoxin production are varied and complex, ranging from oxidative stress to specific inhibition of enzymatic components of mycotoxin biosynthetic pathways. This review presents natural and synthetic antifungal AMPs from different origins which are effective against mycotoxin-producing fungi, and aims at summarizing current knowledge concerning their additional effects on mycotoxin biosynthesis. Antifungal AMPs properties and mechanisms of action are also discussed.
Effect of Cobalt and Chromium Ions on the Chlorhexidine Digluconate as Seen by Intermolecular Diffusion
Authors: Fangaia Sónia I. G. , Nicolau Pedro M. G. , Guerra Fernando A. D. R. A. , Rodrigo M. Melia , Utzeri Gianluca , Cabral Ana M. T. D. P. V. , Valente Artur J. M. , Esteso Miguel A. , Ribeiro Ana C. F.
Published: December 2021

Metal ions such as cobalt (II) and chromium (III) might be present in the oral cavity, as a consequence of the corrosion of Co-Cr dental alloys. The diffusion of such metal ions into the organism, carried by saliva, can cause health problems as a consequence of their toxicity, enhanced by a cumulative effect in the body. The effect of the chlorhexidine digluconate, which is commonly used in mouthwash formulations, on the transport of these salts is evaluated in this paper by using the Taylor dispersion technique, which will allow an assessment of how the presence of chlorhexidine digluconate (either in aqueous solution or in a commercial formulation) may affect the diffusion of metal ions. The ternary mutual diffusion coefficients of metal ions (Co and Cr) in the presence of chlorhexidine digluconate, in an artificial saliva media, were measured. Significant coupled diffusion of CoCl2 (and CrCl3) and chlorhexidine digluconate is observed by analysis of the non-zero values of the cross-diffusion coefficients, D12 and D21. The observed interactions between metal ions and chlorhexidine digluconate suggest that the latter might be considered as an advantageous therapeutic agent, once they contribute to the reduction of the concentration of those ions inside the mouth.
Effect of NK-5962 on Gene Expression Profiling of Retina in a Rat Model of Retinitis Pigmentosa
Authors: Liu Shihui , Miyaji Mary , Hosoya Osamu , Matsuo Toshihiko
Published: December 2021

Purpose: NK-5962 is a key component of photoelectric dye-coupled polyethylene film, designated Okayama University type-retinal prosthesis (OUReP™). Previously, we found that NK-5962 solution could reduce the number of apoptotic photoreceptors in the eyes of the Royal College of Surgeons (RCS) rats by intravitreal injection under a 12 h light/dark cycle. This study aimed to explore possible molecular mechanisms underlying the anti-apoptotic effect of NK-5962 in the retina of RCS rats. Methods: RCS rats received intravitreal injections of NK-5962 solution in the left eye at the age of 3 and 4 weeks, before the age of 5 weeks when the speed in the apoptotic degeneration of photoreceptors reaches its peak. The vehicle-treated right eyes served as controls. All rats were housed under a 12 h light/dark cycle, and the retinas were dissected out at the age of 5 weeks for RNA sequence (RNA-seq) analysis. For the functional annotation of differentially expressed genes (DEGs), the Metascape and DAVID databases were used. Results: In total, 55 up-regulated DEGs, and one down-regulated gene (LYVE1) were found to be common among samples treated with NK-5962. These DEGs were analyzed using Gene Ontology (GO) term enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome pathway analyses. We focused on the up-regulated DEGs that were enriched in extracellular matrix organization, extracellular exosome, and PI3K–Akt signaling pathways. These terms and pathways may relate to mechanisms to protect photoreceptor cells. Moreover, our analyses suggest that SERPINF1, which encodes pigment epithelium-derived factor (PEDF), is one of the key regulatory genes involved in the anti-apoptotic effect of NK-5962 in RCS rat retinas. Conclusions: Our findings suggest that photoelectric dye NK-5962 may delay apoptotic death of photoreceptor cells in RCS rats by up-regulating genes related to extracellular matrix organization, extracellular exosome, and PI3K–Akt signaling pathways. Overall, our RNA-seq and bioinformatics analyses provide insights in the transcriptome responses in the dystrophic RCS rat retinas that were induced by NK-5962 intravitreal injection and offer potential target genes for developing new therapeutic strategies for patients with retinitis pigmentosa.
Role of Endothelial Glucocorticoid Receptor in the Pathogenesis of Kidney Diseases
Authors: Przybyciński Jarosław , Drożdżal Sylwester , Domański Leszek , Dziedziejko Violetta , Pawlik Andrzej
Published: December 2021

Glucocorticoids, as multifunctional hormones, are widely used in the treatment of various diseases including nephrological disorders. They are known to affect immunological cells, effectively treating many autoimmune and inflammatory processes. Furthermore, there is a growing body of evidence demonstrating the potent role of glucocorticoids in non-immune cells such as podocytes. Moreover, novel data show additional pathways and processes affected by glucocorticoids, such as the Wnt pathway or autophagy. The endothelium is currently considered as a key organ in the regulation of numerous kidney functions such as glomerular filtration, vascular tone and the regulation of inflammation and coagulation. In this review, we analyse the literature concerning the effects of endothelial glucocorticoid receptor signalling on kidney function in health and disease, with special focus on hypertension, diabetic kidney disease, glomerulopathies and chronic kidney disease. Recent studies demonstrate the potential role of endothelial GR in the prevention of fibrosis of kidney tissue and cell metabolism through Wnt pathways, which could have a protective effect against disease progression. Another important aspect covered in this review is blood pressure regulation though GR and eNOS. We also briefly cover potential therapies that might affect the endothelial glucocorticoid receptor and its possible clinical implications, with special interest in selective or local GR stimulation and potential mitigation of GC treatment side effects.
High-Throughput Profiling of Cas12a Orthologues and Engineered Variants for Enhanced Genome Editing Activity
Authors: Zhu Dan , Wang Junyi , Yang Di , Xi Jianzhong , Li Juan
Published: December 2021

CRISPR/Cas12a (formerly Cpf1), an RNA-guided endonuclease of the Class II Type V-A CRISPR system, provides a promising tool for genome engineering. Over 10 Cas12a orthologues have been identified and employed for gene editing in human cells. However, the functional diversity among emerging Cas12a orthologues remains poorly explored. Here, we report a high-throughput comparative profiling of editing activities across 16 Cas12a orthologues in human cells by constructing genome-integrated, self-cleaving, paired crRNA–target libraries containing >40,000 guide RNAs. Three Cas12a candidates exhibited promising potential owing to their compact structures and editing efficiency comparable with those of AsCas12a and LbCas12a, which are well characterized. We generated three arginine substitution variants (3Rv) via structure-guided protein engineering: BsCas12a-3Rv (K155R/N512R/K518R), PrCas12a-3Rv (E162R/N519R/K525R), and Mb3Cas12a-3Rv (D180R/N581R/K587R). All three Cas12a variants showed enhanced editing activities and expanded targeting ranges (NTTV, NTCV, and TRTV) compared with the wild-type Cas12a effectors. The base preference analysis among the three Cas12a variants revealed that PrCas12a-3Rv shows the highest activity at target sites with canonical PAM TTTV and non-canonical PAM TTCV, while Mb3Cas12a-3Rv exhibits recognition features distinct from the others by accommodating for more nucleotide A at position −3 for PAM TATV and at position −4 for PAM ATCV. Thus, the expanded Cas12a toolbox and an improved understanding of Cas12a activities should facilitate their use in genome engineering.
Long-Term Sex- and Genotype-Specific Effects of 56Fe Irradiation on Wild-Type and APPswe/PS1dE9 Transgenic Mice
Authors: Schroeder Maren K. , Liu Bin , Hinshaw Robert G. , Park Mi-Ae , Wang Shuyan , Dubey Shipra , Liu Grace Geyu , Shi Qiaoqiao , Holton Peter , Reiser Vladimir , Jones Paul A. , Trigg William , Di Carli Marcelo F. , Caldarone Barbara J. , Williams Jacqueline P. , O’Banion M. Kerry , Lemere Cynthia A.
Published: December 2021

Space radiation presents a substantial threat to travel beyond Earth. Relatively low doses of high-energy particle radiation cause physiological and behavioral impairments in rodents and may pose risks to human spaceflight. There is evidence that 56Fe irradiation, a significant component of space radiation, may be more harmful to males than to females and worsen Alzheimer’s disease pathology in genetically vulnerable models. Yet, research on the long-term, sex- and genotype-specific effects of 56Fe irradiation is lacking. Here, we irradiated 4-month-old male and female, wild-type and Alzheimer’s-like APP/PS1 mice with 0, 0.10, or 0.50 Gy of 56Fe ions (1GeV/u). Mice underwent microPET scans before and 7.5 months after irradiation, a battery of behavioral tests at 11 months of age and were sacrificed for pathological and biochemical analyses at 12 months of age. 56Fe irradiation worsened amyloid-beta (Aβ) pathology, gliosis, neuroinflammation and spatial memory, but improved motor coordination, in male transgenic mice and worsened fear memory in wild-type males. Although sham-irradiated female APP/PS1 mice had more cerebral Aβ and gliosis than sham-irradiated male transgenics, female mice of both genotypes were relatively spared from radiation effects 8 months later. These results provide evidence for sex-specific, long-term CNS effects of space radiation.
Development of Blended-Learning-Based Semester Credit System Implementation Model to Improve Learning Service
Authors: Wahjono Hari , Wiyono Bambang Budi , Maisyaroh  , Mustiningsih
Published: December 2021

This study aims to describe the implementation of online learning that is applied today, and to develop a blended learning model as a strategy to provide student services according to the diversity of potential, needs, interests, and learning speeds. This study used research and development design. Several stages were carried out, namely, conducting literature studies, planning, organizing, mapping basic competencies, carrying out learning activities, reporting, and dissemination. The preliminary research samples included 10,466 students, and the samples for the blended learning model trial included 144 students through random sampling. The results showed that many students were happy to study at home, whereas others were happy to study at school. The facilities to support online learning at home were sufficient. The online learning presented by the teachers was quite interesting and most parents support it. Based on these findings, a blended learning model was developed. The results of the model trial show that blended learning is very useful and there is a significant increase in student learning outcomes. The blended learning model is very effective at supporting online learning. It can provide optimal services to students according to their talents, interests, and abilities. For that, a special strategy is needed and the cooperation of all the elements of the school. The development of an IT-based management system is very helpful in monitoring the progress of students and increasing the awareness of teachers and parents in overseeing the success of learning. The blended learning model in high school can be one of the models for schools that implement a semester credit system.
A Multiclass Nonparallel Parametric-Margin Support Vector Machine
Authors: Du Shu-Wang , Zhang Ming-Chuan , Chen Pei , Sun Hui-Feng , Chen Wei-Jie , Shao Yuan-Hai
Published: December 2021

The twin parametric-margin support vector machine (TPMSVM) is an excellent kernel-based nonparallel classifier. However, TPMSVM was originally designed for binary classification, which is unsuitable for real-world multiclass applications. Therefore, this paper extends TPMSVM for multiclass classification and proposes a novel K multiclass nonparallel parametric-margin support vector machine (MNP-KSVC). Specifically, our MNP-KSVC enjoys the following characteristics. (1) Under the “one-versus-one-versus-rest” multiclass framework, MNP-KSVC encodes the complicated multiclass learning task into a series of subproblems with the ternary output {−1,0,+1}. In contrast to the “one-versus-one” or “one-versus-rest” strategy, each subproblem not only focuses on separating the two selected class instances but also considers the side information of the remaining class instances. (2) MNP-KSVC aims to find a pair of nonparallel parametric-margin hyperplanes for each subproblem. As a result, these hyperplanes are closer to their corresponding class and at least one distance away from the other class. At the same time, they attempt to bound the remaining class instances into an insensitive region. (3) MNP-KSVC utilizes a hybrid classification and regression loss joined with the regularization to formulate its optimization model. Then, the optimal solutions are derived from the corresponding dual problems. Finally, we conduct numerical experiments to compare the proposed method with four state-of-the-art multiclass models: Multi-SVM, MBSVM, MTPMSVM, and Twin-KSVC. Experimental results demonstrate the feasibility and effectiveness of MNP-KSVC in terms of multiclass accuracy and learning time.
Oilseed Rape Shares Abundant and Generalized Pollinators with Its Co-Flowering Plant Species
Authors: Thompson Amibeth , Ștefan Valentin , Knight Tiffany M.
Published: December 2021

Mass-flowering crops, such as Oilseed Rape (OSR), provide resources for pollinators and benefit from pollination services. Studies that observe the community of interactions between plants and pollinators are critical to understanding the resource needs of pollinators. We observed pollinators on OSR and wild plants in adjacent semi-natural areas in Sachsen-Anhalt, Germany to quantify (1) the co-flowering plants that share pollinators with OSR, (2) the identity and functional traits of plants and pollinators in the network module of OSR, and (3) the identity of the plants and pollinators that act as network connectors and hubs. We found that four common plants share a high percentage of their pollinators with OSR. OSR and these plants all attract abundant pollinators in the community, and the patterns of sharing were not more than would be expected by chance sampling. OSR acts as a module hub, and primarily influences the other plants in its module that have similar functional traits. However, the plants that most influence the pollination of OSR have different functional traits and are part of different modules. Our study demonstrates that supporting the pollination of OSR requires the presence of semi-natural areas with plants that can support a high abundances of generalist pollinators.
Numerical Research on Global Ice Loads of Maneuvering Captive Motion in Level Ice
Authors: Xuan Shenyu , Zhan Chengsheng , Liu Zuyuan , Zhao Qiaosheng , Guo Wei
Published: December 2021

In level ice, the maneuvering motion of icebreakers has a major influence on the global ice loads of the hull. This study researched the influences of the drift angle and turning radius on the ice loads of the icebreaker Xue Long through a partial numerical method based on the linear superposition theory of ice loads. First, with reference to the Araon model tests performed by the Korea Research Institute of Ships and Ocean Engineering (KRISO), numerical simulations of Araon’s direct motion were carried out at different speeds, and the average deviation between numerical results and model test results was about 13.8%. Meanwhile, the icebreaking process and modes were analyzed and discussed, compared with a model test and a full-scale ship trial. Next, the maneuvering captive motions of oblique and constant radius were simulated to study the characteristics of ice loads under different drift angles and turning radii. Compared with the maneuvering motion model tests in the ice tank of Tianjin University and the Institute for Ocean Technology of the National Research Council of Canada (NRC/IOT), the numerical results had good agreement with the model test results in terms of the variation trend of ice loads and ice–hull interaction, and the influences of drift angle and turning radius on ice resistance and transverse force, which have a certain reference value for sailing performance research and the design of the hull form of icebreaker ships, are discussed.
Risk-Profile and Feature Selection Comparison in Diabetic Retinopathy
Authors: Maeda-Gutiérrez Valeria , Galván-Tejada Carlos E. , Cruz Miguel , Galván-Tejada Jorge I. , Gamboa-Rosales Hamurabi , García-Hernández Alejandra , Luna-García Huizilopoztli , Gonzalez-Curiel Irma , Martínez-Acuña Mónica
Published: December 2021

One of the main microvascular complications presented in the Mexican population is diabetic retinopathy which affects 27.50% of individuals with type 2 diabetes. Therefore, the purpose of this study is to construct a predictive model to find out the risk factors of this complication. The dataset contained a total of 298 subjects, including clinical and paraclinical features. An analysis was constructed using machine learning techniques including Boruta as a feature selection method, and random forest as classification algorithm. The model was evaluated through a statistical test based on sensitivity, specificity, area under the curve (AUC), and receiving operating characteristic (ROC) curve. The results present significant values obtained by the model obtaining 69% of AUC. Moreover, a risk evaluation was incorporated to evaluate the impact of the predictors. The proposed method identifies creatinine, lipid treatment, glomerular filtration rate, waist hip ratio, total cholesterol, and high density lipoprotein as risk factors in Mexican subjects. The odds ratio increases by 3.5916 times for control patients which have high levels of cholesterol. It is possible to conclude that this proposed methodology is a preliminary computer-aided diagnosis tool for clinical decision-helping to identify the diagnosis of DR.
COVID-19 and Venous Thromboembolism: From Pathological Mechanisms to Clinical Management
Authors: Zhou Xianghui , Cheng Zhipeng , Hu Yu
Published: December 2021

Coronavirus disease 2019 (COVID-19), which is becoming a global pandemic, is caused by SARS-CoV-2 infection. In COVID-19, thrombotic events occur frequently, mainly venous thromboembolism (VTE), which is closely related to disease severity and clinical prognosis. Compared with historical controls, the occurrence of VTE in hospitalized and critical COVID-19 patients is incredibly high. However, the pathophysiology of thrombosis and the best strategies for thrombosis prevention in COVID-19 remain unclear, thus needing further exploration. Virchow’s triad elements have been proposed as important risk factors for thrombotic diseases. Therefore, the three factors outlined by Virchow can also be applied to the formation of venous thrombosis in the COVID-19 setting. A thorough understanding of the complex interactions in these processes is important in the search for effective treatments for COVID-19. In this work, we focus on the pathological mechanisms of VTE in COVID-19 from the aspects of endothelial dysfunction, hypercoagulability, abnormal blood flow. We also discuss the treatment of VTE as well as the ongoing clinical trials of heparin anticoagulant therapy. In addition, according to the pathophysiological mechanism of COVID-19-associated thrombosis, we extended the range of antithrombotic drugs including antiplatelet drugs, antifibrinolytic drugs, and anti-inflammatory drugs, hoping to find effective drug therapy and improve the prognosis of VTE in COVID-19 patients.
Diabetic Retinopathy and Skin Tissue Advanced Glycation End Products Are Biomarkers of Cardiovascular Events in Type 2 Diabetic Patients
Authors: Planas Alejandra , Simó-Servat Olga , Hernández Cristina , Ortiz-Zúñiga Ángel , Marsal Joan Ramón , Herance José R. , Ferreira-González Ignacio , Simó Rafael
Published: December 2021

Risk of cardiovascular events is not homogeneous in subjects with type 2 diabetes; therefore, its early identification remains a challenge to be met. The aim of this study is to evaluate whether the presence of diabetic retinopathy and accumulation of advanced glycation end-products in subcutaneous tissue can help identify patients at high risk of cardiovascular events. For this purpose, we conducted a prospective study (mean follow-up: 4.35 years) comprising 200 subjects with type 2 diabetes with no history of clinical cardiovascular disease and 60 non-diabetic controls matched by age and sex. The primary outcome was defined as the composite of myocardial infarction, coronary revascularization, stroke, lower limb amputation or cardiovascular death. The Cox proportional hazard multiple regression analysis was used to determine the independent predictors of cardiovascular events. The patients with type 2 diabetes had significantly more cardiovascular events than the non-diabetic subjects. Apart from the classic factors such as age, sex and coronary artery calcium score, we observed that the diabetic retinopathy and advanced glycation end-products in subcutaneous tissue were independent predictors of cardiovascular events. We conclude that the diabetic retinopathy and advanced glycation end-products in subcutaneous tissue could be useful biomarkers for selecting type 2 diabetic patients in whom the screening for cardiovascular disease should be prioritized, thereby creating more personalized and cost-effective medicine.
2-(2-(4-Methoxyphenyl)-4,9-dimethyl-7-oxo-7H-furo[2,3-f]chromen-3-yl)acetic Acid
Authors: Lichitsky Boris V. , Komogortsev Andrey N. , Melekhina Valeriya G.
Published: December 2021

For the first time, we describe a new approach towards the synthesis of previously unknown 2-(2-(4-methoxyphenyl)-4,9-dimethyl-7-oxo-7H-furo[2,3-f]chromen-3-yl)acetic acid. The presented method is based on the multicomponent condensation of 5-hydroxy-4,7-dimethyl-2H-chromen-2-one, 4-methoxyphenylglyoxal and Meldrum’s acid. It was shown that the studied reaction proceeds in two steps including the initial interaction of starting materials in MeCN and the final formation of furylacetic acid moiety in acidic media. The structures of the obtained compound were established by 1H, 13C-NMR spectroscopy and high-resolution mass spectrometry.
The Role of Different Alkali Metals in the A15Tl27 Type Structure and the Synthesis and X-ray Structure Analysis of a New Substitutional Variant Cs14.53Tl28.4
Authors: Schwinghammer Vanessa F. , Tiefenthaler Susanne M. , Gärtner Stefanie
Published: December 2021

Alkali metal thallides have been known since the report of E. Zintl on NaTl in 1932. Subsequently, binary and ternary thallides of alkali metals have been characterized. At an alkali metal proportion of approximately 33% (A:Tl~1:2, A = alkali metal), three different unique type structures are reported: K49Tl108, Rb17Tl41 and A15Tl27 (A = Rb, Cs). Whereas Rb17Tl41 and K49Tl108 feature a three-dimensional sublattice of Tl atoms, the A15Tl27 structure type includes isolated Tl11 clusters as well as two-dimensional Tl-layers. This unique arrangement is only known so far when the heavier alkali metals Rb and Cs are included. In our contribution, we present single-crystal X-ray structure analyses of new ternary and quaternary compounds of the A15Tl27 type structure, which include different amounts of potassium. The crystal structures allow for the discussion of the favored alkali metal for each of the four Wyckoff positions and clearly demonstrate alkali metal dependent site preferences. Thereby, the compound Cs2.27K12.73Tl27 unambiguously proves the possibility of a potassium-rich A15Tl27 phase, even though a small amount of cesium appears to be needed for the stabilization of the latter structure type. Furthermore, we also present two compounds that show an embedding of Tl instead of alkali metal into the two-dimensional substructure, being equivalent to the formal oxidation of the latter. Cs14.53Tl28.4 represents the binary compound with the so far largest proportion of incorporated Tl in the structure type A15Tl27.
FORC-Diagram Analysis for a Step-like Magnetization Reversal in Nanopatterned Stripe Array
Authors: Belyaev Victor K. , Murzin Dmitry , Martínez-García Jose C. , Rivas Montserrat , Andreev Nikolay V. , Kozlov Aleksei G. , Samardak Aleksei Yu. , Ognev Alexey V. , Samardak Alexander S. , Rodionova Valeria
Published: December 2021

The fabrication approach of a magnonic crystal with a step-like hysteresis behavior based on a uniform non-monotonous iron layer made by shadow deposition on a preconfigured substrate is reported. The origin of the step-like hysteresis loop behavior is studied with local and integral magnetometry methods, including First-Order Reversal Curves (FORC) diagram analysis, accompanied with magnetic microstructure dynamics measurements. The results are validated with macroscopic magnetic properties and micromagnetic simulations using the intrinsic switching field distribution model. The proposed fabrication method can be used to produce magnonic structures with the controllable hysteresis plateau region’s field position and width that can be used to control the magnonic crystal’s band structure by changing of an external magnetic field.
Effect of Vulcanization Process Parameters on the Tensile Strength of Carcass of Textile-Rubber Reinforced Conveyor Belts
Authors: Lemmi Tsegaye Sh. , Barburski Marcin , Kabzinski Adam , Frukacz Krzysztof
Published: December 2021

Textile-reinforced conveyor belts are most widely used in various industries, including in the mining, construction, and manufacturing industries, to transport materials from one place to another. The conveyor belt’s tensile strength, which primarily relies on the property of the carcass, determines the area of application of the belt. The main aim of the current work was to investigate the influence of vulcanization temperature and duration of the vulcanization process on the tensile properties of the carcass part of the conveyor belt. An extensive experiment was carried out on the tensile properties of woven fabrics that were intended to reinforce conveyor belts by aging the fabrics at the temperature of 140 °C, 160 °C, and 220 °C for six and thirty-five minutes of aging durations. Afterward, the textile-reinforced conveyor belts were produced at vulcanization temperatures of 140 °C, 160 °C, and 220 °C for six and thirty-five minutes of vulcanizing durations. The influence of the vulcanization process parameters on the tensile property of fabrics utilized for the reinforcement of the conveyor belt was analyzed. In addition, the effect of the dipping process of woven fabric in resorcinol–formaldehyde–latex on the tensile property of polyester/polyamide 66 woven fabric (EP fabric) was investigated. The investigation results revealed that the tensile strength of the carcass of the conveyor belt was significantly affected by vulcanization temperature. The conveyor belt vulcanized at 160 °C for 35 min has shown the optimum tensile strength, which is 2.22% and 89.06% higher than the samples vulcanized at 140 °C and 220 °C for 35 min, respectively. Furthermore, the tensile strength and percentage elongation at break of conveyor belts vulcanized at 220 °C were almost destroyed regardless of the vulcanization duration.
Epitaxial Growth of Diamond-Shaped Au1/2Ag1/2CN Nanocrystals on Graphene
Authors: Park Chunggeun , Ham Jimin , Heo Yun Jung , Lee Won Chul
Published: December 2021

Epitaxial synthesis of inorganic nanomaterials on pristine 2D materials is of interest in the development of nanostructured devices and nanocomposite materials, but is quite difficult because pristine surfaces of 2D materials are chemically inert. Previous studies found a few exceptions including AuCN, AgCN, CuCN, and Cu0.5Au0.5CN, which can be preferentially synthesized and epitaxially aligned onto various 2D materials. Here, we discover that Au1/2Ag1/2CN forms diamond-shaped nanocrystals epitaxially grown on pristine graphene surfaces. The nanocrystals synthesized by a simple drop-casting method are crystallographically aligned to lattice structures of the underlying graphene. Our experimental investigations on 3D structures and the synthesis conditions of the nanocrystals imply that the rhombic 2D geometries originate from different growth rates depending on orientations along and perpendicular to 1D molecular chains of Au1/2Ag1/2CN. We also perform in situ TEM observations showing that Au1/2Ag1/2CN nanocrystals are decomposed to Au and Ag alloy nanocrystals under electron beam irradiation. Our experimental results provide an additional example of 1D cyanide chain families that form ordered nanocrystals epitaxially aligned on 2D materials, and reveal basic physical characteristics of this rarely investigated nanomaterial.
Enhanced Strength and Plasticity of CoCrNiAl0.1Si0.1 Medium Entropy Alloy via Deformation Twinning and Microband at Cryogenic Temperature
Authors: Gu Xiao-Hua , Meng Yu-Quan , Chang Hui , Bai Tian-Xiang , Ma Sheng-Guo , Zhang Yong-Qiang , Song Wei-Dong , Li Zhi-Qiang
Published: December 2021

The synthesis of lightweight yet strong-ductile materials has been an imperative challenge in alloy design. In this study, the CoCrNi-based medium-entropy alloys (MEAs) with added Al and Si were manufactured by vacuum arc melting furnace subsequently followed by cool rolling and anneal process. The mechanical responses of CoCrNiAl0.1Si0.1 MEAs under quasi-static (1 × 10−3 s−1) tensile strength showed that MEAs had an outstanding balance of yield strength, ultimate tensile strength, and elongation. The yield strength, ultimate tensile strength, and elongation were increased from 480 MPa, 900 MPa, and 58% at 298 K to 700 MPa, 1250 MPa, and 72% at 77 K, respectively. Temperature dependencies of the yield strength and strain hardening were investigated to understand the excellent mechanical performance, considering the contribution of lattice distortions, deformation twins, and microbands. Severe lattice distortions were determined to play a predominant role in the temperature-dependent yield stress. The Peierls barrier height increased with decreasing temperature, owing to thermal vibrations causing the effective width of a dislocation core to decrease. Through the thermodynamic formula, the stacking fault energies were calculated to be 14.12 mJ/m2 and 8.32 mJ/m2 at 298 K and 77 K, respectively. In conclusion, the enhanced strength and ductility at cryogenic temperature can be attributed to multiple deformation mechanisms including dislocations, extensive deformation twins, and microbands. The synergistic effect of multiple deformation mechanisms lead to the outstanding mechanical properties of the alloy at room and cryogenic temperature.