bluuebunny/crossref_metadata_2025 huggingface dataset:
Strike slip motion and the triggering of subduction initiation
Authors: Li Yida , Gurnis Michael
Published: April 2023

Plate tectonic reconstructions of three of the best-defined Cenozoic subduction initiation (SI) events in the western Pacific, Izu-Bonin-Mariana, Vanuatu, and Puysegur subduction zones, show substantial components of strike-slip motion before and during the subduction initiation. Using computational models, we show that strike-slip motion has a large influence on the effective strength of incipient margins and the ease of subduction initiation. The parameter space associated with visco-elasto-plastic rheologies, plate weakening, and plate forces and kinematics is explored and we show that subduction initiates more easily with a higher force, a faster weakening, or greater strike-slip motion. With the analytical solution, we demonstrate that the effect of strike-slip motion can be equivalently represented by a modified weakening rate. Along transpressive margins, we show that a block of oceanic crust can become trapped between a new thrust fault and the antecedent strike-slip fault and is consistent with structural reconstructions and gravity models of the Puysegur margin. Together, models and observations suggest that subduction initiation can be triggered when margins become progressively weakened to the point that the resisting forces become smaller than the driving forces, and as the negative buoyancy builds up, the intraplate stress eventually turns from compressional into extensional. The analytical formulation of the initiation time, tSI, marking the moment when intraplate stress flips sign, is validated with a computational models. The analytical solution shows that tSI is dominated by convergence velocity, while the plate age, strike-slip velocity, and weakening rate all have a smaller but still important effect on the time scale of subduction initiation.
Judging similarity versus judging difference
Authors: Humphry Stephen , Montuoro Paul
Published: April 2023

A key feature of the polytomous Rasch model is that it entails a single classification process into one of a set of ordered response categories. The first aim of this article is to make explicit two processes in rubric designs that bring about such classifications, and identify implications of the distinction between them. The first process involves judgements of similarity, typified by judgements that a performance is similar to a category description. The second process involves judgements of difference. It is typified by judgements that a performance is better than a category description. In this article, each process is explicated using a hypothetical context in which assessors compare performances with exemplars. The second aim is to demonstrate that, for reporting purposes, judgements of similarity are recommended, particularly when results are interpreted by persons with limited knowledge of the Rasch model and its parameters. In judgements of similarity, category descriptions are located at the peak of each category probability curve, at the point of highest probability in each the category, with thresholds located between them. This is easier to interpret than judgements of difference, where category descriptions are located at the thresholds, and where the point of highest probability has no material reference. This is explicated using a simple real-world example. Implications for rubric design are discussed, as well as implications for related types of instruments, such as attitudinal measures and pairwise comparisons.
NeuroStratE: An educational neuroscience intervention to reduce procrastination behavior and improve executive planning function in higher students
Authors: Cherrier Sophie , Wattelez Guillaume , Ferrière Séverine , Borst Grégoire
Published: April 2023

This study aimed to evaluate the impact of an educational cognitive neuroscience intervention (NeuroStratE) focusing on teaching the functioning of the brain and practical tools to address the procrastination behavior of 199 students enrolled at university from 2019 to 2021. The evolution of procrastination behavior is measured by specific scales and the planning ability through the Tower of Hanoi test. We compared the change in procrastination behaviors and planning ability between the pre and post-test to those of a control group. Procrastination behavior reduction and planning ability improved more in the students who followed the intervention (N = 179) than those in the control group (N = 20). Using mixed methods, these results were qualitatively refined with student feedback on the value of the intervention program, along with individual student interviews. This study highlights that it is particularly courses of procrastination and associated practical tools which contributed to reduced procrastination behavior and improved the planning ability.
Straightforward data transfer in a blockwise dataflow for an analog RRAM-based CIM system
Authors: Liu Yuyi , Gao Bin , Yao Peng , Liu Qi , Zhang Qingtian , Wu Dong , Tang Jianshi , Qian He , Wu Huaqiang
Published: April 2023

Analog resistive random-access memory (RRAM)-based computation-in-memory (CIM) technology is promising for constructing artificial intelligence (AI) with high energy efficiency and excellent scalability. However, the large overhead of analog-to-digital converters (ADCs) is a key limitation. In this work, we propose a novel LINKAGE architecture that eliminates PE-level ADCs and leverages an analog data transfer module to implement inter-array data processing. A blockwise dataflow is further proposed to accelerate convolutional neural networks (CNNs) to speed up compute-intensive layers and solve the unbalanced pipeline problem. To obtain accurate and reliable benchmark results, key component modules, such as straightforward link (SFL) modules and Tile-level ADCs, are designed in standard 28 nm CMOS technology. The evaluation shows that LINKAGE outperforms the conventional ADC/DAC-based architecture with a 2.07×∼11.22× improvement in throughput, 2.45×∼7.00× in energy efficiency, and 22%–51% reduction in the area overhead while maintaining accuracy. Our LINKAGE architecture can achieve 22.9∼24.4 TOPS/W energy efficiency (4b-IN/4b-W) and 1.82 ∼4.53 TOPS throughput with the blockwise method. This work demonstrates a new method for significantly improving the energy efficiency of CIM chips, which can be applied to general CNNs/FCNNs.
Establishment and validation of nomograms to predict survival probability of advanced malignant pleural mesothelioma based on the SEER database and a Chinese medical institution
Authors: Zhang Xuemei , Chang Lele , Zhu Yingying , Mao Yuxin , Zhang Tao , Zhang Qian , Wang Chunbo
Published: April 2023

Objective

The purpose of this study was to build nomograms for predicting the survival of individual advanced pleural mesothelioma (MPM) patients using the Surveillance, Epidemiology, and End Results (SEER) database.

Methods

The 1251 patients enrolled from the SEER database were randomized (in a 7:3 ratio) to a training cohort and an internal validation cohort. Eighty patients were enrolled from the Harbin Medical University Cancer Hospital as the external validation cohort. Nomograms were constructed from variables screened by univariate or multivariate Cox regression analyses and evaluated by consistency indices (C-index), calibration plots, and receiver operating characteristic (ROC) curves. Patients from the SEER database who received chemotherapy alone and chemoradiotherapy were statistically paired using propensity score matching of the two groups and performed subgroup analysis in the screened variables.

Results

The nomograms are well-structured and well-validated prognostic maps constructed from four variables: gender, histology, AJCC stage, and treatment. All individuals were allocated into high-risk versus low-risk groups based on the median risk score of the training cohort, with the high-risk group having worse OS and CSS in all three cohorts (P<0.05). The outcomes of the subgroup analysis indicated that the advanced MPM patients receiving chemotherapy with or without local radiotherapy do not affect OS or CSS.

Conclusion

The accurate nomograms to predict the survival of patients with advanced MPM were built and validated based on an analysis of the SEER database with an external validation cohort. The study suggests that the additional local radiotherapy to chemotherapy does not increase the survival benefit of patients.
Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism
Authors: Yuan Chongshan , Zhang Kaiyan , Wang Zhe , Ma Xin , Liu Hongyu , Zhao Jing , Lu Wenfa , Wang Jun
Published: April 2023

Introduction

Flaxseed oil (FO) and vitamin E (VE) both have antioxidant effects on sperm. The present study investigated the effects of dietary supplementation with FO and/or VE on semen quality.

Methods

16 fertile Simmental bulls were selected and randomly divided into 4 groups (n = 4): the control group (control diet), FO group (control diet containing 24 g/kg FO), VE group (control diet containing 150 mg/kg VE) and FOVE group (control diet containing 150 mg/kg VE and 24 g/kg FO), and the trial lasted 10 weeks.

Results

The results showed that the addition of FO independently can increase sperm motion parameters, the levels of catalase (CAT), glutathione peroxidase (GSH-Px), testosterone (T) and estradiol (E2), while reduce oxidative stress in seminal plasma (P < 0.05). Supplement of VE independently can increased the motility, motility parameters, CAT and superoxide dismutase (SOD) levels, and reduce oxidative stress in seminal plasma (P < 0.05). There was an interaction effect of FO × VE on motility and reactive oxygen species (ROS), while GSH-Px and ROS were affected by week × VE 2-way interaction, levels of T and E2 were also affected by the dietary FO × week interaction (P < 0.05). The triple interaction effects of FO, VE and week were significant for malondialdehyde (MDA) (P < 0.05). Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P < 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.

Conclusion

With in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway.
Diversified crop rotations and organic amendments as strategies for increasing soil carbon storage and stabilisation in UK arable systems
Authors: Zani Caio F. , Manning David A. C. , Abbott Geoffrey D. , Taylor James A. , Cooper Julia , Lopez-Capel Elisa
Published: April 2023

Adaptations in crop rotation with the inclusion of temporary grass-clover leys and organic amendments, have been promoted as effective ways to improve soil carbon (C) sequestration and mitigate climate change in agricultural systems. However, there are still a lot of uncertainties related to i) the combined effects of different crop rotations and different fertilisation sources, e.g., organic amendments, on soil C stocks; and ii) their potential effect on C stabilisation. The objective of this study was to evaluate the effect of different arable crop rotations with varying degrees of diversity in crop type and lengths of grass-clover ley periods and fertilisation sources on soil C stocks and C stabilisation down to 0.60 m soil depth. This was investigated in a long-term factorial field experiment-combining different crop rotation (cereal-intensive conventionalvs.diversified legume-intensive organic) with different lengths of grass-clover ley periods (2vs.3 years), fertilisation sources (mineralvs.compost), and years (samples taken at the beginning and at the last year of one complete cycle of rotation; 8 years apart)-to explore their individual and interactive effect on soil C stock and C stabilisation at two soil depths (0–0.30 and 0.30–0.60 m). Soil C stabilisation was assessed using a unique combination of three different techniques: physical fractionation for separation of C associated to organic and mineral fractions, thermal analysis combined with differential scanning calorimetry and a quadrupole mass spectrometry (TG-DSC-QMS) for physical-chemical aspects, and pyrolysis coupled with gas chromatography-mass spectrometry (Py-GC/MS) for molecular structural information. The findings showed higher soil C stocks under the diversified organic rotation with 3 years of grass-clover ley period at both soil depths, regardless of the fertilisation source or sampling year. However, the organic rotation seemed to deliver stable soil C stocks only in the subsoil layer. Compost fertilisation, in turn, increased topsoil C stocks between the two sample dates under both rotations, and it appears to be stable. These results suggested that combining a diversified organic rotation with 3 years grass-clover ley with compost fertilisation could be one way for agricultural systems to deliver stable soil C sequestration.
The moderating role of sustainable development goals in reviving the economy through green initiatives
Authors: Tang Honglei , Maqbool Asifa , Khan Ali Junaid , Ul Hameed Waseem , Batool Hafsah , Ahmed Tanveer
Published: April 2023

The Ukrainian economic crisis began with the start of the war and grew over time. This is because trade between countries was spread out because of problems with the supply chain. However, the energy sector in Ukraine is largely disturbed by this armed conflict. The purpose of this study was to test the impact of infrastructure damage, trade sanctions, the economic crisis, and energy investments in green energy in Ukraine through the lens of sustainable development goals (SGDs). The data is collected from the Ukrainian public, and the sample size for this research is 384. This research has used the structural equation modeling method by using Smart PLS 3 for data analysis. The findings of the study suggest that a green energy transition is possible when the Ukrainian government works on SDGs to manage the economic crisis and increases investments in the energy sector. The moderation of SGDs is considered a way forward for development in Ukraine. The novelty of this research is based on its framework, which is a new addition to a piece of knowledge. This research has extended knowledge by presenting new relationships related to the green energy transition. The limitations of this research are presented in conjunction with future directions that are a reliable way for scholars to contribute further to this area of research.
Evaluating fence-end treatments for migratory amphibians
Authors: Harman Kristine E. , Omlor Kadie , Popescu Viorel
Published: April 2023

Migratory amphibians require movements to complete their biphasic life cycle, often across altered landscapes fragmented by roadways, which can have severe consequences on their populations. To manage this threat, transportation agencies have begun to implement exclusion fencing to separate natural areas from the roadway to prevent wildlife-vehicle collisions. Although fences are an effective conservation tool, the tendency of animals to access the road by circumventing the fence ends, known as the fence-end effect, threatens to jeopardize management efforts to reduce road associated mortality. One strategy to lessen the impacts of the fence-end effect is to construct fence-end treatments to block amphibian movement and guide the animals to safe crossing locations. By using experimental fence arenas, we examined how nine amphibian species responded to two alternative fence-end structures: horizontal v-shape and perpendicular fence-end treatments. Using a generalized linear model framework, we found both fence-end treatments to be an effective strategy to reduce the impacts of the fence-end effect, with our predictor variable, fence-end treatment, explaining most of the variation in amphibian response. Structure effectiveness also started to improve by 20% with each 7°C increase in temperature, however, this was not significant. Despite these promising findings, we also found for each additional 312 s an amphibians spent attempting to navigate around the experimental fence resulted in a 25% decline in structure effectiveness, suggesting longer fences are not an adequate protection measure to combat the fence-end effect for amphibians. In addition,Anaxyrus americanuswas not found to differ in their response, performing equally well to both experimental fence-end treatments. In contrast,Ranaspp.,Pseudacris crucifer, andNotopthalmus viridescensshowed a greater response to the horizontal v-shape fence-end treatment compared to the perpendicular fence-end treatment. Variation in response forAmbystomaspp. could not be detected due to a small sample size; however, no individuals responded positively to the perpendicular fence-end treatment. Guidelines for amphibian fences should continue to incorporate fence-end treatments into the design and implementation to mitigate for the fence-end effect, and preferably angle the fence-ends inward in the horizontal v-shape pattern with the fence ends diagonal to the road for migratory amphibians.
A review on the role of LINC00511 in cancer
Authors: Ghafouri-Fard Soudeh , Safarzadeh Arash , Hussen Bashdar Mahmud , Taheri Mohammad , Ayatollahi Seyed Abdulmajid
Published: April 2023

Long Intergenic Non-Protein Coding RNA 511 (LINC00511) is an RNA gene being mostly associated with lung cancer. Further assessments have shown dysregulation of this lncRNA in a variety of cancers. LINC00511 has interactions with hsa-miR-29b-3p, hsa-miR-765, hsa-mir-150, miR-1231, TFAP2A-AS2, hsa-miR-185-3p, hsa-miR-29b-1-5p, hsa-miR-29c-3p, RAD51-AS1 and EZH2. A number of transcription factors have been identified that regulate expression of LINC00511. The current narrative review summarizes the role of LINC00511 in different cancers with an especial focus on its prognostic impact in human cancers.
Lifetime violence and suicidal ideation among young women (18–24 years) in Uganda: Results from a population-based survey
Authors: Kisaakye Peter , Kafuko Agatha , Bukuluki Paul
Published: April 2023

Introduction

Violence is a major global public health issue that threatens the physical and mental health of victims. Of particular concern is the increasing evidence which suggests that violence is strongly associated with suicidal behavior including ideation.

Methods

This study uses data from the 2015 Violence Against Children Survey (VACS). This study seeks to highlight the relationship between lifetime violence and suicidal ideation using a nationally representative sample of 1,795 young women (18–24 years) in Uganda.

Results

Results indicate that respondents who experienced lifetime sexual violence (aOR = 1.726; 95%CI = 1.304–2.287), physical violence (aOR = 1.930; 95%CI = 1.293–2.882) or emotional violence (aOR = 2.623; 95%CI = 1.988–3.459) were more likely to experience suicidal ideation. Respondents who were not married (aOR = 1.607; 95%CI = 1.040–2.484), not having too much trust with community members (aOR = 1.542; 95%CI = 1.024–2.320) or not having a close relationship with biological parents (aOR = 1.614; 95%CI = 1.230–2.119) were more likely to experience suicidal ideation. Respondents who did not engage in work in the past 12 months prior to the survey (aOR = 0.629; 95%CI = 0.433–0.913) were less likely to experience suicidal ideation.

Conclusion

The results can be used to inform policy and programming and for integration of mental health and psychosocial support in programming for prevention and response to violence against young women.
Should treatment of hypogammaglobulinemia with immunoglobulin replacement therapy (IgRT) become standard of care in patients with chronic lymphocytic leukemia?
Authors: Noto Alessandro , Cassin Ramona , Mattiello Veronica , Bortolotti Marta , Reda Gianluigi , Barcellini Wilma
Published: April 2023

Hypogammaglobulinemia (HGG) is a frequent finding in patients with hematological malignancies, and is commonly described in chronic lymphocytic leukemia (CLL) before or after treatment. We reviewed published literature available online in the last thirty years through Medline search of indexed articles focusing on the main differences and advantages of the products now available on the market, namely intravenous Ig (IVIg) and subcutaneous Ig (SCIg) preparations. IgRT is effective and safe in the prophylaxis of infections in a selected group of patients with CLL and hypogammaglobulinemia and is therefore a valuable tool for clinicians in the everyday management of infectious risk. We encourage the use of SCIg formulations as they appear to have similar efficacy but better cost-effectiveness and tolerability.
The TNF-α rs361525 and IFN-γ rs2430561 polymorphisms are associated with liver cirrhosis risk: a comprehensive meta-analysis
Authors: Zheng Minghui , Li Jing , Fang Weizhen , Luo Ling , Ding Rui , Zeng Hua , Luo Hong , Lin Xianghua , Duan Chaohui
Published: April 2023

Background

Inflammation serves as an essential driver of liver cirrhosis (LC) incidence. Accordingly, a meta-analysis was carried out to explore the association between specific polymorphisms in the interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) genes and the incidence of LC based on comparisons of genotype and allele frequencies.

Objectives

To study the relationship between TNF-α rs361525 and IFN-γ rs2430561 polymorphisms and the risk of LC.

Methods

A database search was performed for all studies published as of September 10, 2022. The strength of risk relationships was assessed based on odds ratios (ORs) with 95% confidence intervals (CIs).

Results

Pooled analyses were conducted for one common TNF-α polymorphism (rs361525) as well as one common IFN-γ polymorphism (rs2430561). Both of these SNPs were identified as LC-related risk factors. Specifically, rs361525 was related to LC incidence in both alcoholic liver cirrhosis (OR: 1.86, 95%CI: 1.03-3.34) and hepatitis B virus (HBV)-related cirrhosis cases (OR: 1.44, 95%CI: 1.00-2.06) when using an allelic contrast model. Moreover, rs2430561 was significantly related to LC in an Asian population (OR: 1.45, 95%CI: 1.13-1.86) and in the context of HBV-related cirrhosis (OR: 1.48, 95%CI: 1.13-1.93) when using an allelic contrast model.

Conclusion

These findings indicate that rs361525 and rs2430561 represent LC-related risk factors, although additional large-scale clinical and case-control studies will be vital to confirm these results.
Identification of immune-associated genes in diagnosing osteoarthritis with metabolic syndrome by integrated bioinformatics analysis and machine learning
Authors: Li Junchen , Wang Genghong , Xv Xilin , Li Zhigang , Shen Yiwei , Zhang Cheng , Zhang Xiaofeng
Published: April 2023

Background

In the pathogenesis of osteoarthritis (OA) and metabolic syndrome (MetS), the immune system plays a particularly important role. The purpose of this study was to find key diagnostic candidate genes in OA patients who also had metabolic syndrome.

Methods

We searched the Gene Expression Omnibus (GEO) database for three OA and one MetS dataset. Limma, weighted gene co-expression network analysis (WGCNA), and machine learning algorithms were used to identify and analyze the immune genes associated with OA and MetS. They were evaluated using nomograms and receiver operating characteristic (ROC) curves, and finally, immune cells dysregulated in OA were investigated using immune infiltration analysis.

Results

After Limma analysis, the integrated OA dataset yielded 2263 DEGs, and the MetS dataset yielded the most relevant module containing 691 genes after WGCNA, with a total of 82 intersections between the two. The immune-related genes were mostly enriched in the enrichment analysis, and the immune infiltration analysis revealed an imbalance in multiple immune cells. Further machine learning screening yielded eight core genes that were evaluated by nomogram and diagnostic value and found to have a high diagnostic value (area under the curve from 0.82 to 0.96).

Conclusion

Eight immune-related core genes were identified (FZD7, IRAK3, KDELR3, PHC2, RHOB, RNF170, SOX13, and ZKSCAN4), and a nomogram for the diagnosis of OA and MetS was established. This research could lead to the identification of potential peripheral blood diagnostic candidate genes for MetS patients who also suffer from OA.
The long-term antibody response after SARS-CoV-2 prime-boost vaccination in healthy individuals. The positive influence of extended between-dose intervals and heterologous schedule
Authors: Naidich Gretel , Santucci Natalia E. , Pezzotto Stella Maris , Ceccarelli Eduardo A. , Bottasso Oscar A. , Perichón A. Mario
Published: April 2023

Introduction

Anti-COVID vaccination in Argentina was carried out using different protocols and variations in periods between administrations, as well as combinations of different vaccine platforms. Considering the relevance of the antibody response in viral infections, we analyzed anti-S antibodies in healthy people at different points of time following the Sputnik immunization procedure.

Methods

We attended the vaccination centers in the city of Rosario, which had shorter versus longer intervals between both doses. A total of (1021) adults with no COVID-compatible symptoms (throughout the study period) were grouped according to the gap between both vaccine doses: 21 (Group A, n=528), 30 (Group B, n=147), and 70 days (Group C, n=82), as well as an additional group of individuals with heterologous vaccination (Sputnik/Moderna, separated by a 107-day interval, group D, n=264).

Results and conclusions

While there were no between-group differences in baseline levels of specific antibodies, data collected several weeks after administering the second dose showed that group D had the highest amounts of specific antibodies, followed by values recorded in Groups C, B, and A. The same pattern of group differences was seen when measuring anti-S antibodies at 21 or 180 days after the first and second doses, respectively. Delayed between-dose intervals coexisted with higher antibody titers. This happened even more when using a prime-boost heterologous schedule.
A crucial exosome-related gene pair (AAMP and ABAT) is associated with inflammatory cells in intervertebral disc degeneration
Authors: Ren Huiyong , Li Yumin , Liu Hao , Fan Jiaxin , Li Jie , Li Haopeng , Wei Hongyu , Meng Liesu , Cao Shuai
Published: April 2023

Identification of exosome-related genes (ERGs) and competing endogenous RNAs (ceRNAs) associated with intervertebral disc degeneration (IDD) may improve its diagnosis and reveal its underlying mechanisms. We downloaded 49 samples from Gene Expression Omnibus and identified candidate ERGs using differentially expressed ERGs (De-ERGs), exosome-related gene pairs (ERGPs), and machine learning algorithms [least absolute shrinkage and selection operator (LASSO) and support vector machine (SVM)]. Immune cell-related ERGs were selected via immune-infiltration analysis, and clinical values were assessed using receiver operating characteristic curves. Based on the De-ERGs, a ceRNA network comprising 1,512 links and 330 nodes was constructed and primarily related to signal transduction pathways, apoptosis-related biological processes, and multiple kinase-related molecular functions. In total, two crucial De-ERGs [angio-associated migratory cell protein (AAMP) and 4-aminobutyrate aminotransferase (ABAT)] were screened from results in De-ERGs, ERGPs, LASSO, and SVM. Increased AAMP expression and decreased ABAT expression were positively and negatively correlated with CD8+ T cell infiltration, respectively. AAMP/ABAT was the only pair differentially expressed in IDD and correlated with CD8+ T cell infiltration. Furthermore, AAMP/ABAT displayed higher accuracy in predicting IDD than individual genes. These results demonstrated the ERGP AAMP/ABAT as a robust signature for identifying IDD and associated with increased CD8+ T cell infiltration, suggesting it as a promising IDD biomarker.
Case report: Immune modulation after PD-1 inhibitor therapy in a patient with extranodal NK/T-cell lymphoma secondary to chronic active Epstein-Barr virus disease unveiled by single-cell transcriptomics
Authors: Wang Yao , Zhang Minan , Xue Qingfeng , Zhou Huan , Chen Jie , Wang Hong , Zhang Yaping , Shi Wenyu
Published: April 2023

Chronic active Epstein-Barr virus disease (CAEBV) is a systemic lymphoproliferative disorder that is closely linked to Epstein-Barr virus (EBV) infection. The clinical course and severity of CAEBV can vary, and in some cases, it can progress to overt lymphoma, which is characterized by extranodal natural killer/T-cell lymphoma (ENKTL) and has a poor clinical outcome. Although anti-programmed cell death protein-1 (PD-1) therapy has shown effectiveness in some patients with EBV-associated disease, it has been less successful in others, and the exact mechanism of action of PD-1 inhibitor therapy in these diseases remains unclear. In this report, we describe a patient who was diagnosed with ENKTL secondary to CAEBV and experienced rapid disease progression accompanied by hyperinflammation after receiving PD-1 inhibitor therapy. Single-cell RNA sequencing revealed a significant increase in the patient’s lymphocyte count, especially in natural killer cells, with increased activity following PD-1 inhibitor therapy. This case raises questions about the efficacy and safety of PD-1 inhibitor therapy in patients with EBV-associated diseases.
Reasonable restrictions on the freedom of fishing in high seas marine protected areas from an international law perspective: an analysis
Authors: Wang Yong , Pan Xin
Published: April 2023

With the development of marine protected areas (MPAs) on the high seas in the international community and an international agreement protecting biodiversity beyond national jurisdiction, how to reasonably restrict the freedom of fishing in the high seas MPAs has become a controversial issue in theory and in practice. This article will use doctrinal research method. It will discuss existing provisions regarding restricting the freedom of fishing on the high seas including the high seas MPAs. These provisions have been codified in international treaties, international resolutions, and international soft law. In addition, since regional fisheries management organizations (RFMOs) also take part in fishery management in the high seas MPAs, this article will refer to measures of relevant RFMOs to discuss how restriction is going in terms of the freedom of fish in the high seas MPAs. This article finds that the fishing-restriction measures stipulated in international documents represented by the United Nations Convention on the Law of the Sea are too general. In addition, some treaties do not address certain issues of restricting fishing methods by non-contracting parties. Therefore, these treaties are limited. The fishing practices of the four high seas MPAs that have been established by the international community reveals the establishment of several reasonable fishing restrictions. However, their shortcomings are evident. From the perspective of international law, it is important to improve the principle of reasonably restricting the freedom of fishing in MPAs on the high seas based on practice and positive law while proceeding steadily. Moreover, restriction measures cannot be too broad or narrow. Specifically, we should continue to develop mechanisms such as exceptions, ways of restricting fishing, advocacy provisions for non- contracting parties and measures to strengthen international information exchange.
Hyperbaric oxygen therapy as rescue therapy for pediatric frosted branch angiitis with Purtscher-like retinopathy: A case report
Authors: Lee Chi-Tai , Hsieh Tzu-Han , Chu Chan-Ching , Hsu Yung-Ray , Wang Jia-Horng , Wang Jia-Kang , Zhao Zhanqi , Chang Hou-Tai
Published: April 2023

Introduction

Frosted branch angiitis (FBA) is an uncommon uveitis characterized by fulminant retinal vasculitis. Purtscher-like retinopathy (PuR) is a rare retinal angiopathy associated with a non-traumatic etiology. Both FBA and PuR can cause profound visual impairments.

Case report

We describe the case of a 10-year-old male who presented with sudden bilateral painless visual loss due to FBA with concurrent PuR, with notable viral prodrome 1 month prior to presentation. Systemic investigations revealed a recent herpes simplex virus 2 infection with a high titer of IgM, positive antinuclear antibody (ANA) (1:640), and abnormal liver function tests. After administration of systemic corticosteroids, anti-viral agents, and subsequent immunosuppressive medications, the FBA was gradually alleviated. However, fundoscopy and optical coherence tomography (OCT) revealed persistent PuR and macular ischemia. Hence, hyperbaric oxygen therapy was administered as a rescue strategy, which resulted in gradual bilateral visual acuity improvement.

Conclusion

Hyperbaric oxygen therapy may be a beneficial rescue treatment for retinal ischemia secondary to FBA with PuR.
Immunotherapies against human bacterial and fungal infectious diseases: A review
Authors: Qadri Hafsa , Shah Abdul Haseeb , Alkhanani Mustfa , Almilaibary Abdullah , Mir Manzoor Ahmad
Published: April 2023

Nations’ ongoing struggles with a number of novel and reemerging infectious diseases, including the ongoing global health issue, the SARS-Co-V2 (severe acute respiratory syndrome coronavirus 2) outbreak, serve as proof that infectious diseases constitute a serious threat to the global public health. Moreover, the fatality rate in humans is rising as a result of the development of severe infectious diseases brought about by multiple drug-tolerant pathogenic microorganisms. The widespread use of traditional antimicrobial drugs, immunosuppressive medications, and other related factors led to the establishment of such drug resistant pathogenic microbial species. To overcome the difficulties commonly encountered by current infectious disease management and control processes, like inadequate effectiveness, toxicities, and the evolution of drug tolerance, new treatment solutions are required. Fortunately, immunotherapies already hold great potential for reducing these restrictions while simultaneously expanding the boundaries of healthcare and medicine, as shown by the latest discoveries and the success of drugs including monoclonal antibodies (MAbs), vaccinations, etc. Immunotherapies comprise methods for treating diseases that specifically target or affect the body’s immune system and such immunological procedures/therapies strengthen the host’s defenses to fight those infections. The immunotherapy-based treatments control the host’s innate and adaptive immune responses, which are effective in treating different pathogenic microbial infections. As a result, diverse immunotherapeutic strategies are being researched more and more as alternative treatments for infectious diseases, leading to substantial improvements in our comprehension of the associations between pathogens and host immune system. In this review we will explore different immunotherapies and their usage for the assistance of a broad spectrum of infectious ailments caused by various human bacterial and fungal pathogenic microbes. We will discuss about the recent developments in the therapeutics against the growing human pathogenic microbial diseases and focus on the present and future of using immunotherapies to overcome these diseases.

Graphical Abstract

The graphical abstract shows the therapeutic potential of different types of immunotherapies like vaccines, monoclonal antibodies-based therapies, etc., against different kinds of human Bacterial and Fungal microbial infections.
Personalised therapeutic approaches to glioblastoma: A systematic review
Authors: Mowforth Oliver D. , Brannigan Jamie , El Khoury Marc , Sarathi Celine Iswarya Partha , Bestwick Harry , Bhatti Faheem , Mair Richard
Published: April 2023

Introduction

Glioblastoma is the most common and malignant primary brain tumour with median survival of 14.6 months. Personalised medicine aims to improve survival by targeting individualised patient characteristics. However, a major limitation has been application of targeted therapies in a non-personalised manner without biomarker enrichment. This has risked therapies being discounted without fair and rigorous evaluation. The objective was therefore to synthesise the current evidence on survival efficacy of personalised therapies in glioblastoma.

Methods

Studies reporting a survival outcome in human adults with supratentorial glioblastoma were eligible. PRISMA guidelines were followed. MEDLINE, Embase, Scopus, Web of Science and the Cochrane Library were searched to 5th May 2022.Clinicaltrials.govwas searched to 25th May 2022. Reference lists were hand-searched. Duplicate title/abstract screening, data extraction and risk of bias assessments were conducted. A quantitative synthesis is presented.

Results

A total of 102 trials were included: 16 were randomised and 41 studied newly diagnosed patients. Of 5,527 included patients, 59.4% were male and mean age was 53.7 years. More than 20 types of personalised therapy were included: targeted molecular therapies were the most studied (33.3%, 34/102), followed by autologous dendritic cell vaccines (32.4%, 33/102) and autologous tumour vaccines (10.8%, 11/102). There was no consistent evidence for survival efficacy of any personalised therapy.

Conclusion

Personalised glioblastoma therapies remain of unproven survival benefit. Evidence is inconsistent with high risk of bias. Nonetheless, encouraging results in some trials provide reason for optimism. Future focus should address target-enriched trials, combination therapies, longitudinal biomarker monitoring and standardised reporting.
Determinants of SARS-CoV-2 Contagiousness in Household Contacts of Symptomatic Adult Index Cases
Authors: Trunfio Mattia , Richiardi Lorenzo , Alladio Francesca , Staffilano Elena , Longo Bianca , Venuti Francesco , Ghisetti Valeria , Burdino Elisa , Bonora Stefano , Vineis Paolo , Di Perri Giovanni , Calcagno Andrea
Published: April 2023

Background

Identifying determinants of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) transmission in settings of contagion is fundamental to inform containment strategies. We assessed SARS-CoV-2 cycle threshold value (Ct) from the first diagnostic nasal–pharyngeal swab of symptomatic index cases and which demographic or clinical characteristics among cases and contacts are associated with transmission risk within households.

Methods

This is a retrospective prevalence study on secondary SARS-CoV-2 cases (SC) among the household contacts of symptomatic adult index cases randomly sampled from all the SARS-CoV-2-positive diagnostic nasopharyngeal swabs analyzed at our regional referral hospital (Amedeo di Savoia Hospital, Turin, Italy) in March, 2020. Index cases underwent a telephone survey to collect their demographic and clinical data and all their household contacts. The Ct value of RdRp gene from the first diagnostic swab of index cases was recorded and index cases were grouped according to Ct tertiles (A < first tertile, first ≤ B ≤ second tertile, C ≥ second tertile). Post hoc analysis was performed in SC as well as contacts that did not undergo SARS-CoV-2 testing but developed compatible signs and symptoms. Non-parametric tests and generalized linear models were run.

Results

Index (n = 72) and contact (n = 164) median age was 54 (48–63) and 32 (20–56) years, respectively. A total of 60, 50, and 54 subjects were contacts of group A, B, and C index cases, respectively; 35.9% of contacts were SC. Twenty-four further subjects (14.6%) met the criteria for symptom-based likely positive SC. The secondary attack rate was 36.0% (28.6–43.4), assuming a mean incubation period of 5 days and a maximum infectious period of 20 days. SC prevalence differed between Ct groups (53.3% A, 32.0% B, 20.4% C; p < 0.001). No difference in SC was found according to sex, presence of signs/symptoms, and COVID-19 severity of index cases, or according to contacts’ sex and number per household. The age of both index cases [aOR 4.52 (1.2–17.0) for 60 vs. ≤45 years old] and contacts [aOR 3.66 (1.3–10.6) for 60 vs. ≤45years old] and the Ct of the index [aOR 0.17 (0.07–0.4) for Ct ≥ 31.8 vs. Ct < 24.4] independently associated with SC risk. Sensitivity analysis including symptoms-based likely positive SC supported all the previous results.

Conclusion

In confined transmission settings such as households, PCR Ct values may inform on the contagiousness of infected subjects and age may modulate transmission/contagion risk.
Screening and characterization of Bacillus velezensis LB-Y-1 toward selection as a potential probiotic for poultry with multi-enzyme production property
Authors: Li Chong , Li Shuzhen , Dang Guoqi , Jia Rui , Chen Si , Deng Xuejuan , Liu Guohua , Beckers Yves , Cai Huiyi
Published: April 2023

Bacillus spp. have gained increasing recognition as an option to use as antimicrobial growth promoters, which are characterized by producing various enzymes and antimicrobial compounds. The present study was undertaken to screen and evaluate a Bacillus strain with the multi-enzyme production property for poultry production. LB-Y-1, screened from the intestines of healthy animals, was revealed to be a Bacillus velezensis by the morphological, biochemical, and molecular characterization. The strain was screened out by a specific screening program, possessed excellent multi-enzyme production potential, including protease, cellulase, and phytase. Moreover, the strain also exhibited amylolytic and lipolytic activity in vitro. The dietary LB-Y-1 supplementation improved growth performance and tibia mineralization in chicken broilers, and increased serum albumin and serum total protein at 21 days of age (p < 0.05). Besides, LB-Y-1 enhanced the activity of serum alkaline phosphatase and digestive enzyme in broilers at 21 and 42 days of age (p < 0.05). Analysis of intestinal microbiota showed that a higher community richness (Chao1 index) and diversity (Shannon index) in the LB-Y-1 supplemented compared with the CON group. PCoA analysis showed that the community composition and structure were distinctly different between the CON and LB-Y-1 group. The beneficial genera such as Parasutterella and Rikenellaceae were abundant, while the opportunistic pathogen such as Escherichia-Shigella were reduced in the LB-Y-1 supplemented group (p < 0.05). Collectively, LB-Y-1 can be considered as a potential strain for further utilization in direct-fed microbial or starter culture for fermentation.
Evaluation of DNA extraction methods and direct PCR in metabarcoding of mock and marine bacterial communities
Authors: Stojan Iva , Trumbić Željka , Lepen Pleić Ivana , Šantić Danijela
Published: April 2023

Recent advances in new molecular biology methods and next-generation sequencing (NGS) technologies have revolutionized metabarcoding studies investigating complex microbial communities from various environments. The inevitable first step in sample preparation is DNA extraction which introduces its own set of biases and considerations. In this study, we assessed the influence of five DNA extraction methods [B1: phenol/chloroform/isoamyl extraction, B2 and B3: isopropanol and ethanol precipitations, respectively—both modifications of B1, K1: DNeasy PowerWater Kit (QIAGEN), K2: modified DNeasy PowerWater Kit (QIAGEN) and direct PCR approach (P) that completely circumvents this step on community composition and DNA yield of mock and marine sample communities from the Adriatic Sea]. B1–B3 methods generally produced higher DNA yields and more similar microbial communities, but with higher interindividual variability. Each method demonstrated significant differences in a specific community structure, where rare taxa seem to play a crucial role. There was not one superior method closest to the theoretically expected mock community composition, they all demonstrated skewed ratios, but in a similar way which might be attributed to other factors, such as primer bias or 16S rRNA gene count for specific taxa. Direct PCR represents an interesting approach when high throughput in sample processing is required. We emphasize the importance of making a cautious decision about the choice of the extraction method or direct PCR approach, but even more importantly its consistent application throughout the study.
Clinical characteristics and microbiota analysis of 44 patients with granulomatous mastitis
Authors: Chen Wen , Zhang Dongxiao , Zeng Yifei , Cui Jianchun , Yu Jiale , Wang Junyue , Li Shuqi , Huang Qiao , Mansoor Khattak Mazher
Published: April 2023

Introduction

Granulomatous mastitis (GM) is a chronic inflammatory breast disease. In recent years, the role of Corynebacterium in GM onset has received more and more attention. This study aims to detect the dominant bacterium in GM patients and analyze the association between clinical characteristics and infectious factors.

Methods

In this study, 88 samples from 44 GM patients, six acute lactation mastitis (ALM) patients, and 25 non-inflammatory breast disease (NIB) patients were divided into a GM pus group, a GM tissue group, an ALM pus group, and a NIB tissue group; then, 16S ribosomal DNA sequencing was used to explore their microbiota. The clinical data of all 44 GM patients were also retrospectively collected and analyzed to determine their relationship with infection.

Results

The median age of the 44 GM patients was 33 years, and 88.6% of patients had primary-onset cases, while 11.4% were recurrences; additionally, 89.5% of patients were postpartum and 10.5% were nulliparous. The serum prolactin level was abnormal in nine patients (24.3%). Samples from 15 GM patients (34.1%) had a Corynebacterium abundance of >1% (1.08–80.08%), with eight (53.3%) displaying an abundance of >10%. Corynebacterium was the only genus with significant differences between the GM pus group and the other three groups (p < 0.05). Corynebacterium kroppenstedtii was the predominant Corynebacterium species. Among clinical characteristics, a statistical difference in breast abscess formation was observed according to Corynebacterium abundance in Corynebacterium-positive and- negative patients (p < 0.05).

Discussion

This study explored the relationship between Corynebacterium infection and GM, compared the clinical characteristics between Corynebacterium-positive and- negative patients, and provided support for the role of Corynebacterium species-in particular, C. kroppenstedtii-in the pathogenesis of GM. The detection of Corynebacterium can predict GM onset, especially in patients with high prolactin levels or a history of recent lactation.