bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantifying the Effect of Building Shadowing and Cloudiness on Mean Radiant Temperature in Singapore
Authors: Acero Juan A. , Koh Elliot J. Y. , Tan Yon Sun , Norford Leslie K.
Published: August 2021

Improving the quality of life in urban areas has become a major concern in the last few decades. With a constantly increasing urban population and in a climate change context, detailed knowledge of the impact of urban elements on the outdoor thermal environment is relevant. In this work, we present the results of several climatic campaigns carried out in Singapore aiming to evaluate local urban climate variables. Sensors were deployed simultaneously in different sites. The effect of building shadowing in the diurnal cycle of mean radiant temperature (Tmrt) is evaluated in different seasons. Although during the Inter-Monsoon season, mean Tmrt reduction due to building shadow is ≈19 °C, during clear skies days, it can be reduced by ≈30 °C. The Tmrt difference between sites is analyzed based on the weather conditions, the sky view factor (SVF), and the type of surrounding urban elements. Under building shadow conditions, higher SVF showed higher Tmrt values, although no correlation was found between Tmrt and diffuse solar radiation (measured above the urban canopy). The results suggest a relevant contribution of other radiation components (e.g., longwave radiation). The quantitative analysis of the Tmrt provided in this work is relevant for outdoor thermal comfort strategies in tropical areas such as Singapore.
Self-Organogenesis from 2D Micropatterns to 3D Biomimetic Biliary Trees
Authors: Gontran Emilie , Loarca Lorena , El Kassis Cyrille , Bouzhir Latifa , Ayollo Dmitry , Mazari-Arrighi Elsa , Fuchs Alexandra , Dupuis-Williams Pascale
Published: August 2021

Background and Aims: Globally, liver diseases account for 2 million deaths per year. For those with advanced liver disease the only curative approach is liver transplantation. However, less than 10% of those in need get a liver transplant due to limited organ availability. To circumvent this challenge, there has been a great focus in generating a bioengineered liver. Despite its essential role in liver functions, a functional biliary system has not yet been developed. In this framework, exploration of epithelial cell self-organogenesis and microengineering-driven geometrical cell confinement allow to envision the bioengineering of a functional biomimetic intrahepatic biliary tract. Approach: three-dimensional (3D) bile ducts were built in vitro by restricting cell adhesion to two-dimensional (2D) patterns to guide cell self-organization. Tree shapes mimicking the configuration of the human biliary system were micropatterned on glass slides, restricting cell attachment to these areas. Different tree geometries and culture conditions were explored to stimulate self-organogenesis of normal rat cholangiocytes (NRCs) used as a biliary cell model, either alone or in co-culture with human umbilical endothelial cells (HUVECs). Results: Pre-seeding the micropatterns with HUVECs promoted luminogenesis with higher efficiency to yield functional branched biliary tubes. Lumen formation, apico-basal polarity, and preservation of the cholangiocyte phenotype were confirmed. Moreover, intact and functional biliary structures were detached from the micropatterns for further manipulation. Conclusion: This study presents physiologically relevant 3D biliary duct networks built in vitro from 2D micropatterns. This opens opportunities for investigating bile duct organogenesis, physiopathology, and drug testing.
Clinical Perspectives of Single-Cell RNA Sequencing
Authors: Kim Nayoung , Eum Hye Hyeon , Lee Hae-Ock
Published: August 2021

The ability of single-cell genomics to resolve cellular heterogeneity is highly appreciated in cancer and is being exploited for precision medicine. In the recent decade, we have witnessed the incorporation of cancer genomics into the clinical decision-making process for molecular-targeted therapies. Compared with conventional genomics, which primarily focuses on the specific and sensitive detection of the molecular targets, single-cell genomics addresses intratumoral heterogeneity and the microenvironmental components impacting the treatment response and resistance. As an exploratory tool, single-cell genomics provides an unprecedented opportunity to improve the diagnosis, monitoring, and treatment of cancer. The results obtained upon employing bulk cancer genomics indicate that single-cell genomics is at an early stage with respect to exploration of clinical relevance and requires further innovations to become a widely utilized technology in the clinic.
CRISPR as a Diagnostic Tool
Authors: Kim Seohyun , Ji Sangmin , Koh Hye Ran
Published: August 2021

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system has recently gained growing attention as a diagnostic tool due to its capability of specific gene targeting. It consists of Cas enzymes and a guide RNA (gRNA) that can cleave the target DNA or RNA based on the sequence of the gRNA, making it an attractive genetic engineering technique. In addition to the target-specific binding and cleavage, the trans-cleavage activity was reported for some Cas proteins, including Cas12a and Cas13a, which is to cleave the surrounding single-stranded DNA or RNA upon the target binding of Cas-gRNA complex. All these activities of the CRISPR-Cas system are based on its target-specific binding, making it applied to develop diagnostic methods by detecting the disease-related gene as well as microRNAs and the genetic variations such as single nucleotide polymorphism and DNA methylation. Moreover, it can be applied to detect the non-nucleic acids target such as proteins. In this review, we cover the various CRISPR-based diagnostic methods by focusing on the activity of the CRISPR-Cas system and the form of the target. The CRISPR-based diagnostic methods without target amplification are also introduced briefly.
Proinflammatory Pathways Are Activated in the Human Q344X Rhodopsin Knock-In Mouse Model of Retinitis Pigmentosa
Authors: Hollingsworth T.J. , Hubbard Meredith G. , Levi Hailey J. , White William , Wang Xiangdi , Simpson Raven , Jablonski Monica M. , Gross Alecia K.
Published: August 2021

Retinitis pigmentosa (RP) is a hereditary disease of the retina that results in complete blindness. Currently, there are very few treatments for the disease and those that exist work only for the recessively inherited forms. To better understand the pathogenesis of RP, multiple mouse models have been generated bearing mutations found in human patients including the human Q344X rhodopsin knock-in mouse. In recent years, the immune system was shown to play an increasingly important role in RP degeneration. By way of electroretinography, optical coherence tomography, funduscopy, fluorescein angiography, and fluorescent immunohistochemistry, we show degenerative and vascular phenotypes, microglial activation, photoreceptor phagocytosis, and upregulation of proinflammatory pathway proteins in the retinas of the human Q344X rhodopsin knock-in mouse. We also show that an FDA-approved pharmacological agent indicated for the treatment of rheumatoid arthritis is able to halt activation of pro-inflammatory signaling in cultured retinal cells, setting the stage for pre-clinical trials using these mice to inhibit proinflammatory signaling in an attempt to preserve vision. We conclude from this work that pro- and autoinflammatory upregulation likely act to enhance the progression of the degenerative phenotype of rhodopsin Q344X-mediated RP and that inhibition of these pathways may lead to longer-lasting vision in not only the Q344X rhodopsin knock-in mice, but humans as well.
Mitogen-Activated Protein Kinase Pathway in Amyotrophic Lateral Sclerosis
Authors: Sahana TG , Zhang Ke
Published: August 2021

Amyotrophic lateral sclerosis is a fatal motor neuron degenerative disease. Multiple genetic and non-genetic risk factors are associated with disease pathogenesis, and several cellular processes, including protein homeostasis, RNA metabolism, vesicle transport, etc., are severely impaired in ALS conditions. Despite the heterogeneity of the disease manifestation and progression, ALS patients show protein aggregates in the motor cortex and spinal cord tissue, which is believed to be at least partially caused by aberrant phase separation and the formation of persistent stress granules. Consistent with this notion, many studies have implicated cellular stress, such as ER stress, DNA damage, oxidative stress, and growth factor depletion, in ALS conditions. The mitogen-activated protein kinase (MAPK) pathway is a fundamental mitogen/stress-activated signal transduction pathway that regulates cell proliferation, differentiation, survival, and death. Here we summarize the fundamental role of MAPK in physiology and ALS pathogenesis. We also discuss pharmacological inhibitors targeting this pathway tested in pre-clinical models, suggesting their role as potential drug candidates.
Myocardial Metabolic Response Predicts Chemotherapy Curative Potential on Hodgkin Lymphoma: A Proof-of-Concept Study
Authors: Marini Cecilia , Bauckneht Matteo , Borra Anna , Lai Rita , Donegani Maria Isabella , Miceli Alberto , Campi Cristina , Cossu Vanessa , Schenone Daniela , Morbelli Silvia , Chauvie Stephane , Piana Michele , Gallamini Andrea , Sambuceti Gianmario
Published: August 2021

Genome sharing between cancer and normal tissues might imply a similar susceptibility to chemotherapy toxicity. The present study aimed to investigate whether curative potential of doxorubicin, bleomycin, vinblastine, and dacarbazine (ABVD) is predicted by the metabolic response of normal tissues in patients with Hodgkin lymphoma (HL). METHODS: According to current guidelines, 86 patients with advanced-stage (IIB-IVB) HL, prospectively enrolled in the HD0607 trial (NCT00795613), underwent 18 F-fluorodeoyglucose PET/CT imaging at diagnosis and, at interim, after two ABVD courses, to decide regimen maintenance or its escalation. In both scans, myocardial FDG uptake was binarized according to its median value. Death and disease relapse were recorded to estimate progression-free survival (PFS) during a follow-up with median duration of 43.8 months (range 6.97–60). RESULTS: Four patients (4.6%) died, while six experienced disease relapse (7%). Complete switch-off of cancer lesions and cardiac lighting predicted a favorable outcome at Kaplan–Mayer analyses. The independent nature and additive predictive value of their risk prediction were confirmed by the multivariate Cox regression analysis. CONCLUSION: Susceptibility of HL lesions to chemotherapy is at least partially determined by factors featuring the host who developed it.
Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis
Authors: Wang Chunxue , Yi Pengfei , Li Jiang , Dong Haibing , Chen Changming , Zhang Daming , Shen Haiqing , Fu Bendong
Published: August 2021

In this work, we successfully developed a fluorinated cross-linked polymer Bragg waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of pulmonary microvascular endothelial cell (PMVEC) apoptosis. Fluorinated photosensitive polymer SU-8 (FSU-8) as the sensing core layer and polymethyl methacrylate (PMMA) as the sensing window cladding were synthesized. The effective drug concentration range (5–10 µg/mL) of ginkgolide A for inhibition of PMVEC apoptosis was analyzed and obtained by pharmacological studies. The structure of the device was optimized to be designed and fabricated by direct UV writing technology. The properties of the biosensor were simulated with various refractive indices of different drug concentrations. The actual sensitivity of the biosensor was measured as 1606.2 nm/RIU. The resolution and detection limit were characterized as 0.05 nm and 3 × 10−5 RIU, respectively. The technique is suitable for safe and accurate detection of effective organic drug dosages of Chinese herbal ingredients.
A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization
Authors: Nimse Satish Balasaheb , Song Keum-Soo , Warkad Shrikant Dashrath , Kim Taisun
Published: August 2021

Highly sensitive (high SBR) and highly specific (high SNP discrimination ratio) DNA hybridization is essential for a biosensor with clinical application. Herein, we propose a method that allows detecting multiple pathogens on a single platform with the SNP discrimination ratios over 160:1 in the dynamic range of 101 to 104 copies per test. The newly developed SWAT method allows achieving highly sensitive and highly specific DNA hybridizations. The detection and discrimination of the MTB and NTM strain in the clinical samples with the SBR and SNP discrimination ratios higher than 160:1 indicate the high clinical applicability of the SWAT.
The Decline of Architects: Can a Computer Design Fine Architecture without Human Input?
Authors: Kołata Joanna , Zierke Piotr
Published: August 2021

Architects are required to have knowledge of current legislation, ergonomics, and the latest technical solutions. In addition, the design process necessitates an appreciation of the quality of the space and a high degree of creativity. However, it is a profession that has undergone significant changes in recent years due to the pressure exerted by the development of information technology. The designs generated by computer algorithms are becoming such a serious part of designers’ work that some are beginning to question whether they are more the work of computers than humans. There are also increasing suggestions that software development will eventually lead to a situation where humans in the profession will become redundant. This review article aims to present the currently used, implemented, and planned computer technologies employed in the design and consider how they affect and will affect the work of architects in the future. It includes opinions of a wide range of experts on the possibility of computer algorithms replacing architects. The ultimate goal of the article is an attempt to answer the question: will computers eliminate the human factor in the design of the future? It also considers the artificial intelligence or communication skills that computer algorithms would require to achieve this goal. The answers to these questions will contribute not only to determining the future of architecture but will also indicate the current condition of the profession. They will also help us to understand the technologies that are making computers capable of increasingly replacing human professions. Despite differing opinions on the possibility of computer algorithms replacing architects, the conclusions indicate that, currently, computers do not have capabilities and skills to achieve this goal. The speed of technological development, especially such technologies as artificial superintelligence, artificial brains, or quantum computers allows us to predict that the replacement of the architect by machines will be unrealistic in coming decades.
Fire Safety in High-Rise Buildings: Is the Stay-Put Tactic a Misjudgement or Magnificent Strategy?
Authors: Arewa Andrew Oyen , Ahmed Abdullahi , Edwards David J. , Nwankwo Chizaram
Published: August 2021

Historically, fire incidents in high-rise buildings reveal that Fire and Rescue Services frequently rely on the stay-put tactic (i.e., occupants of high-rise buildings should remain in their apartments) during an inferno. Recent fire occurrences in high-rise buildings reveal that there are two opposing viewpoints on the stay-put tactic. First, the understanding that the stay-put tactic is a beneficial practice used to protect, control, and facilitate smooth evacuation of occupants during fire incidents. Second, the argument that the stay-put tactic is a misjudgement and futile strategy that leads to fatalities, particularly in high-rise buildings. The aim of this study was to provide awareness and understanding of fire and rescue services use of the stay-put tactic in high-rise buildings. We attempted to answer the questions: is the stay-put tactic a misjudgement or magnificent strategy? The study adopted phenomenological research strategies with various focus groups consisting of seasoned firefighters and survivors with first-hand accounts of stay-put instructions in high-rise buildings. The study also scrutinised three case studies of fire incidents in high-rise buildings in two countries. The study revealed that the stay-put tactic is obsolete; with the potential to cause catastrophic misjudgement, mostly during conflagrations in high-rise buildings. There is a need to advance research on the use of artificial intelligence communication systems and infrared image detectors camera to enhance quick and smooth fire evacuation in high-rise buildings.
Blue Light Improves Photosynthetic Performance and Biomass Partitioning toward Harvestable Organs in Saffron (Crocus sativus L.)
Authors: Moradi Shirin , Kafi Mohsen , Aliniaeifard Sasan , Salami Seyed Alireza , Shokrpour Majid , Pedersen Carsten , Moosavi-Nezhad Moein , Wróbel Jacek , Kalaji Hazem M.
Published: August 2021

Saffron is a valuable plant and one of the most expensive spices worldwide. Nowadays, there is a tendency to produce this crop in indoor plant production systems. However, the production of saffron is restricted by the need for the reproduction of high-quality corms. In this study, we investigated the effect of different ratios of red (R) and blue (B) light spectra (including 100% B (monochromatic B), 75%, 50%, 40%, 25% B, and 0% B (monochromatic R) on the photosynthetic performance and biomass partitioning as well as morphological and biochemical characteristics of saffron. The growth of flower, root, and corm was improved by increasing the proportion of B to R light. B-grown plants were characterized by the highest photosynthetic functionality with efficient electron transport and lower energy dissipation when compared to R-grown plants. B light directed biomass toward the corms and floral organs, while R light directed it toward the leaves. In saffron, the weight of a daughter corm is of great importance since it determines the yield of the next year. As the ratio of B to R light increased, the daughter corms also became heavier, at the cost of reducing their number, though increasing the proportion of B-enhanced antioxidant capacity as well as the activity of ascorbate peroxidase and catalase while superoxide dismutase activity was enhanced in R-grown plants. In conclusion, B light increased the production of high-quality daughter corms and altered biomass partitioning towards harvestable organs (corms and flowers) in saffron plants.
Autophagy and Mitophagy-Related Pathways at the Crossroads of Genetic Pathways Involved in Familial Sarcoidosis and Host-Pathogen Interactions Induced by Coronaviruses
Authors: Pacheco Yves , Valeyre Dominique , El Jammal Thomas , Vallee Maxime , Chevalier Fabien , Lamartine Jérôme , Sigaudo-Roussel Dominique , Verrier Bernard , Israel-Biet Dominique , Freymond Nathalie , Cottin Vincent , Calender Alain
Published: August 2021

Sarcoidosis is a multisystem disease characterized by the development and accumulation of granulomas, the hallmark of an inflammatory process induced by environmental and/or infectious and or genetic factors. This auto-inflammatory disease mainly affects the lungs, the gateway to environmental aggressions and viral infections. We have shown previously that genetic predisposition to sarcoidosis occurring in familial cases is related to a large spectrum of pathogenic variants with, however, a clustering around mTOR (mammalian Target Of Rapamycin)-related pathways and autophagy regulation. The context of the COVID-19 pandemic led us to evaluate whether such genetic defects may increase the risk of a severe course of SARS-CoV2 infection in patients with sarcoidosis. We extended a whole exome screening to 13 families predisposed to sarcoidosis and crossed the genes sharing mutations with the list of genes involved in the SARS-CoV2 host-pathogen protein-protein interactome. A similar analysis protocol was applied to a series of 100 healthy individuals. Using ENRICH.R, a comprehensive gene set enrichment web server, we identified the functional pathways represented in the set of genes carrying deleterious mutations and confirmed the overrepresentation of autophagy- and mitophagy-related functions in familial cases of sarcoidosis. The same protocol was applied to the set of genes common to sarcoidosis and the SARS-CoV2-host interactome and found a significant enrichment of genes related to mitochondrial factors involved in autophagy, mitophagy, and RIG-I-like (Retinoic Acid Inducible Gene 1) Receptor antiviral response signaling. From these results, we discuss the hypothesis according to which sarcoidosis is a model for studying genetic abnormalities associated with host response to viral infections as a consequence of defects in autophagy and mitophagy processes.
Human Mesenchymal Stromal Cells Enhance Cartilage Healing in a Murine Joint Surface Injury Model
Authors: Perry Jade , Roelofs Anke J. , Mennan Claire , McCarthy Helen S. , Richmond Alison , Clark Susan M. , Riemen Anna H. K. , Wright Karina , De Bari Cosimo , Roberts Sally
Published: August 2021

Human umbilical cord (hUC)- or bone marrow (hBM)-derived mesenchymal stromal cells (MSCs) were evaluated as an allogeneic source of cells for cartilage repair. We aimed to determine if they could enhance healing of chondral defects with or without the recruitment of endogenous cells. hMSCs were applied into a focal joint surface injury in knees of adult mice expressing tdTomato fluorescent protein in cells descending from Gdf5-expressing embryonic joint interzone cells. Three experimental groups were used: (i) hUC-MSCs, (ii) hBM-MSCs and (iii) PBS (vehicle) without cells. Cartilage repair was assessed after 8 weeks and tdTomato-expressing cells were detected by immunostaining. Plasma levels of pro-inflammatory mediators and other markers were measured by electrochemiluminescence. Both hUC-MSC (n = 14, p = 0.009) and hBM-MSC (n = 13, p = 0.006) treatment groups had significantly improved cartilage repair compared to controls (n = 18). While hMSCs were not detectable in the repair tissue at 8 weeks post-implantation, increased endogenous Gdf5-lineage cells were detected in repair tissue of hUC-MSC-treated mice. This xenogeneic study indicates that hMSCs enhance intrinsic cartilage repair mechanisms in mice. Hence, hMSCs, particularly the more proliferative hUC-MSCs, could represent an attractive allogeneic cell population for treating patients with chondral defects and perhaps prevent the onset and progression of osteoarthritis.
Differences in a Single Extracellular Residue Underlie Adhesive Functions of Two Zebrafish Aqp0s
Authors: Vorontsova Irene , Hall James E. , Schilling Thomas F. , Nagai Noriaki , Nakazawa Yosuke
Published: August 2021

Aquaporin 0 (AQP0) is the most abundant lens membrane protein, and loss of function in human and animal models leads to cataract formation. AQP0 has several functions in the lens including water transport and adhesion. Since lens optics rely on strict tissue architecture achieved by compact cell-to-cell adhesion between lens fiber cells, understanding how AQP0 contributes to adhesion would shed light on normal lens physiology and pathophysiology. We show in an in vitro adhesion assay that one of two closely related zebrafish Aqp0s, Aqp0b, has strong auto-adhesive properties while Aqp0a does not. The difference appears to be largely due to a single amino acid difference at residue 110 in the extracellular C-loop, which is T in Aqp0a and N in Aqp0b. Similarly, P110 is the key residue required for adhesion in mammalian AQP0, highlighting the importance of residue 110 in AQP0 cell-to-cell adhesion in vertebrate lenses as well as the divergence of adhesive and water permeability functions in zebrafish duplicates.
Renalase: A Multi-Functional Signaling Molecule with Roles in Gastrointestinal Disease
Authors: Pointer Thomas C. , Gorelick Fred S. , Desir Gary V.
Published: August 2021

The survival factor renalase (RNLS) is a recently discovered secretory protein with potent prosurvival and anti-inflammatory effects. Several evolutionarily conserved RNLS domains are critical to its function. These include a 20 aa site that encodes for its prosurvival effects. Its prosurvival effects are shown in GI disease models including acute cerulein pancreatitis. In rodent models of pancreatic cancer and human cancer tissues, increased RNLS expression promotes cancer cell survival but shortens life expectancy. This 37 kD protein can regulate cell signaling as an extracellular molecule and probably also at intracellular sites. Extracellular RNLS signals through a specific plasma membrane calcium export transporter; this interaction appears most relevant to acute injury and cancer. Preliminary studies using RNLS agonists and antagonists, as well as various preclinical disease models, suggest that the immunologic and prosurvival effects of RNLS will be relevant to diverse pathologies that include acute organ injuries and select cancers. Future studies should define the roles of RNLS in intestinal diseases, characterizing the RNLS-activated pathways linked to cell survival and developing therapeutic agents that can increase or decrease RNLS in relevant clinical settings.
The Interdependency and Co-Regulation of the Vitamin D and Cholesterol Metabolism
Authors: Warren Tara , McAllister Roisin , Morgan Amy , Rai Taranjit Singh , McGilligan Victoria , Ennis Matthew , Page Christopher , Kelly Catriona , Peace Aaron , Corfe Bernard M. , Mc Auley Mark , Watterson Steven
Published: August 2021

Vitamin D and cholesterol metabolism overlap significantly in the pathways that contribute to their biosynthesis. However, our understanding of their independent and co-regulation is limited. Cardiovascular disease is the leading cause of death globally and atherosclerosis, the pathology associated with elevated cholesterol, is the leading cause of cardiovascular disease. It is therefore important to understand vitamin D metabolism as a contributory factor. From the literature, we compile evidence of how these systems interact, relating the understanding of the molecular mechanisms involved to the results from observational studies. We also present the first systems biology pathway map of the joint cholesterol and vitamin D metabolisms made available using the Systems Biology Graphical Notation (SBGN) Markup Language (SBGNML). It is shown that the relationship between vitamin D supplementation, total cholesterol, and LDL-C status, and between latitude, vitamin D, and cholesterol status are consistent with our knowledge of molecular mechanisms. We also highlight the results that cannot be explained with our current knowledge of molecular mechanisms: (i) vitamin D supplementation mitigates the side-effects of statin therapy; (ii) statin therapy does not impact upon vitamin D status; and critically (iii) vitamin D supplementation does not improve cardiovascular outcomes, despite improving cardiovascular risk factors. For (iii), we present a hypothesis, based on observations in the literature, that describes how vitamin D regulates the balance between cellular and plasma cholesterol. Answering these questions will create significant opportunities for advancement in our understanding of cardiovascular health.
Current Progress of Magnetoresistance Sensors
Authors: Yang Songlin , Zhang Jin
Published: August 2021

Magnetoresistance (MR) is the variation of a material’s resistivity under the presence of external magnetic fields. Reading heads in hard disk drives (HDDs) are the most common applications of MR sensors. Since the discovery of giant magnetoresistance (GMR) in the 1980s and the application of GMR reading heads in the 1990s, the MR sensors lead to the rapid developments of the HDDs’ storage capacity. Nowadays, MR sensors are employed in magnetic storage, position sensing, current sensing, non-destructive monitoring, and biomedical sensing systems. MR sensors are used to transfer the variation of the target magnetic fields to other signals such as resistance change. This review illustrates the progress of developing nanoconstructed MR materials/structures. Meanwhile, it offers an overview of current trends regarding the applications of MR sensors. In addition, the challenges in designing/developing MR sensors with enhanced performance and cost-efficiency are discussed in this review.
Functionalization Strategies of PEDOT and PEDOT:PSS Films for Organic Bioelectronics Applications
Authors: Fenoy Gonzalo E. , Azzaroni Omar , Knoll Wolfgang , Marmisollé Waldemar A.
Published: August 2021

Organic bioelectronics involves the connection of organic semiconductors with living organisms, organs, tissues, cells, membranes, proteins, and even small molecules. In recent years, this field has received great interest due to the development of all kinds of devices architectures, enabling the detection of several relevant biomarkers, the stimulation and sensing of cells and tissues, and the recording of electrophysiological signals, among others. In this review, we discuss recent functionalization approaches for PEDOT and PEDOT:PSS films with the aim of integrating biomolecules for the fabrication of bioelectronics platforms. As the choice of the strategy is determined by the conducting polymer synthesis method, initially PEDOT and PEDOT:PSS films preparation methods are presented. Later, a wide variety of PEDOT functionalization approaches are discussed, together with bioconjugation techniques to develop efficient organic-biological interfaces. Finally, and by making use of these approaches, the fabrication of different platforms towards organic bioelectronics devices is reviewed.
Mass Balance Sensitivity and Future Projections of Rabots Glaciär, Sweden
Authors: Taveirne Moon , Ekemar Laura , González Sánchez Berta , Axelsson Josefine , Zhang Qiong
Published: August 2021

Glacier mass balance is heavily influenced by climate, with responses of individual glaciers to various climate parameters varying greatly. In northern Sweden, Rabots Glaciär’s mass balance has decreased since it started being monitored in 1982. To relate Rabots Glaciär’s mass balance to changes in climate, the sensitivity to a range of parameters is computed. Through linear regression of mass balance with temperature, precipitation, humidity, wind speed and incoming radiation the climate sensitivity is established and projections for future summer mass balance are made. Summer mass balance is primarily sensitive to temperature at −0.31 m w.e. per °C change, while winter mass balance is mainly sensitive to precipitation at 0.94 m w.e. per % change. An estimate using summer temperature sensitivity projects a dramatic decrease in summer mass balance to −3.89 m w.e. for the 2091–2100 period under climate scenario RCP8.5. With large increases in temperature anticipated for the next century, more complex modelling studies of the relationship between climate and glacier mass balance is key to understanding the future development of Rabots Glaciär.
Prevalence and Characteristics of Thyroid Abnormalities and Its Association with Anemia in ASIR Region of Saudi Arabia: A Cross-Sectional Study
Authors: Alqahtani Saif Aboud M.
Published: August 2021

The thyroid gland plays a significant role in the metabolism and proliferation of blood cells; hematological disorders are frequently observed in patients with thyroid disorders, and the most frequent problem is anemia. The main objective of this research work is to evaluate the prevalence and types of thyroid dysfunction and their association with anemia in different gender stratified by age in the Asir region of Saudi Arabia. Four years of data from July 2016 to July 2020 for 9992 study subjects were collected. Statistical analysis was performed based on thyroid disorder and anemia stratified by gender and age subgroup. The mean age of the study subject was 43.4 ± 15.8 years, and females constituted 61.7% of cases. Thyroid dysfunction was observed in 49.76% (4973), and subclinical hypothyroidism was the most prevalent type (3922/9992), followed by primary hypothyroidism (530/9992). Females have a significantly higher overall prevalence of thyroid dysfunction than males (p < 0.05). Anemia was detected in 1344 females and 465 males with a thyroid disorder, and also, the prevalence was significantly higher (p < 0.05), compared to the normal thyroid group. Thyroid disorders are a common problem in our population, more prevalent in females than males, with the peak age of above 30 years, and are associated with an increased prevalence of anemia.
Convergence of Coupling-Parameter Expansion-Based Solutions to Ornstein–Zernike Equation in Liquid State Theory
Authors: Menon S. V. G.
Published: August 2021

The objective of this paper is to investigate the convergence of coupling-parameter expansion-based solutions to the Ornstein–Zernike equation in liquid state theory. The analytically solved Baxter’s adhesive hard sphere model is analyzed first by using coupling-parameter expansion. It was found that the expansion provides accurate approximations to solutions—including the liquid-vapor phase diagram—in most parts of the phase plane. However, it fails to converge in the region where the model has only complex solutions. Similar analysis and results are obtained using analytical solutions within the mean spherical approximation for the hardcore Yukawa potential. However, numerical results indicate that the expansion converges in all regions in this model. Next, the convergence of the expansion is analyzed for the Lennard-Jones potential by using an accurate density-dependent bridge function in the closure relation. Numerical results are presented which show convergence of correlation functions, compressibility versus density profiles, etc., in the single as well as two-phase regions. Computed liquid-vapor phase diagrams, using two independent schemes employing the converged profiles, compare excellently with simulation data. The results obtained for the generalized Lennard-Jones potential, with varying repulsive exponent, also compare well with the simulation data. Solution-spaces and the bifurcation of the solutions of the Ornstein–Zernike equation that are relevant to coupling-parameter expansion are also briefly discussed. All of these results taken together establish the coupling-parameter expansion as a practical tool for studying single component fluid phases modeled via general pair-potentials.
Liquid-Based Screening Tests Results: HPV, Liquid-Based Cytology, and P16/Ki67 Dual-Staining in Private-Based Opportunistic Cervical Cancer Screening
Authors: Trzeszcz Martyna , Mazurec Maciej , Jach Robert , Mazurec Karolina , Jach Zofia , Kotkowska-Szeps Izabela , Kania Magdalena , Wantuchowicz Mariola , Prokopyk Anna , Barcikowski Piotr , Przybylski Marcin , Wach Joanna , Halon Agnieszka
Published: August 2021

The baseline data from the private-based opportunistic cervical cancer screening with HRHPV14, liquid-based cytology (LBC) and p16/Ki67 testing, and its quality assessment/quality control (QA/QC) tools are lacking. The age-stratified analysis of 30,066 screening tests results in a Polish population, including the investigation of HRHPV14 status, LBC, and p16/Ki67 dual-staining reporting rates, along with immediate histopathologic correlations, was conducted. For cytopathologic QA/QC, the College of American Pathologists (CAP) benchmarks and enhanced safety protocol were used. The NILM/ASC-US/LSIL/ASC-H/HSIL/AGC reporting rates were 93.9/3.4/2.0/0.22/0.24/0.11, respectively, with correlating HRHPV14-positive rates of 8.4/48.9/77.2/84.6/90.7/26.7. The reporting rates for HSIL (CIN2+) in HRHPV-positive women with NILM/ASC-US/LSIL/ASC-H/HSIL/AGC referred for a colposcopy with biopsy were 19.1/25.8/22.5/12.4/19.1/1.1% of the total HSIL (CIN2+). In total, of the 1130 p16/Ki67 tests, 30% were positive. In NILM HRHPV14-positive women with available histology result, HSIL(CIN2+) was detected in 28.3% of cases. In the first such large-scale Polish study presenting HRHPV14, informed LBC and HSIL (CIN2+) results, the reporting rates were highly consistent with data from American and other CAP-certified laboratories, confirming the possibility of using the 2019 ASCCP risk-based guidelines as one of the screening strategies outside of the US, in conditions of proper QA/QC. The private-based screening model can be effective in cervical cancer prevention, particularly in countries with low population coverage of public funds-based systems.
Alavi–Carlsen Calcification Score (ACCS): A Simple Measure of Global Cardiac Atherosclerosis Burden
Authors: Saboury Babak , Edenbrandt Lars , Piri Reza , Gerke Oke , Werner Tom , Arbab-Zadeh Armin , Alavi Abass , Høilund-Carlsen Poul Flemming
Published: August 2021

Multislice cardiac CT characterizes late stage macrocalcification in epicardial arteries as opposed to PET/CT, which mirrors early phase arterial wall changes in epicardial and transmural coronary arteries. With regard to tracer, there has been a shift from using mainly 18F-fluorodeoxyglucose (FDG), indicating inflammation, to applying predominantly 18F-sodium fluoride (NaF) due to its high affinity for arterial wall microcalcification and more consistent association with cardiovascular risk factors. To make NaF-PET/CT an indispensable adjunct to clinical assessment of cardiac atherosclerosis, the Alavi–Carlsen Calcification Score (ACCS) has been proposed. It constitutes a global assessment of cardiac atherosclerosis burden in the individual patient, supported by an artificial intelligence (AI)-based approach for fast observer-independent segmentation. Common measures for characterizing epicardial coronary atherosclerosis by NaF-PET/CT as the maximum standardized uptake value (SUV) or target-to-background ratio are more versatile, error prone, and less reproducible than the ACCS, which equals the average cardiac SUV. The AI-based approach ensures a quick and easy delineation of the entire heart in 3D to obtain the ACCS expressing ongoing global cardiac atherosclerosis, even before it gives rise to CT-detectable coronary calcification. The quantification of global cardiac atherosclerotic burden by the ACCS is suited for management triage and monitoring of disease progression with and without intervention.
MRI of the Entire Spinal Cord—Worth the While or Waste of Time? A Retrospective Study of 74 Patients with Multiple Sclerosis
Authors: Poulsen Esben Nyborg , Olsson Anna , Gustavsen Stefan , Langkilde Annika Reynberg , Oturai Annette Bang , Carlsen Jonathan Frederik
Published: August 2021

Spinal cord lesions are included in the diagnosis of multiple sclerosis (MS), yet spinal cord MRI is not mandatory for diagnosis according to the latest revisions of the McDonald Criteria. We investigated the distribution of spinal cord lesions in MS patients and examined how it influences the fulfillment of the 2017 McDonald Criteria. Seventy-four patients with relapsing-remitting MS were examined with brain and entire spinal cord MRI. Sixty-five patients received contrast. The number and anatomical location of MS lesions were assessed along with the Expanded Disability Status Scale (EDSS). A Chi-square test, Fischer’s exact test, and one-sided McNemar’s test were used to test distributions. MS lesions were distributed throughout the spinal cord. Diagnosis of dissemination in space (DIS) was increased from 58/74 (78.4%) to 67/74 (90.5%) when adding cervical spinal cord MRI to brain MRI alone (p = 0.004). Diagnosis of dissemination in time (DIT) was not significantly increased when adding entire spinal cord MRI to brain MRI alone (p = 0.04). There was no association between the number of spinal cord lesions and the EDSS score (p = 0.71). MS lesions are present throughout the spinal cord, and spinal cord MRI may play an important role in the diagnosis and follow-up of MS patients.
Early Clinical Experience and Mentoring of Young Dental Students—A Qualitative Study
Authors: Moore Rod , Molsing Simone , Meyer Nicola , Schepler Matilde
Published: August 2021

The literature reports that student transition between preclinical and clinical dental education can be traumatic and stressful for many reasons. Early clinical experience has been reported to provide some relief. In this qualitative study, twelve final year dental students were interviewed about their perceptions and experiences with a mentee/mentor (FOAL) program in Aarhus, Denmark, to see if it (1) counteracted stress perceptions from preclinical education to the clinic, (2) inspired professionalism and a sense of study relevance, (3) helped in learning to reflect on competencies and attitudes, (4) helped with clinical social perspectives (communication/contact), (5) helped with motivation to learn and (6) helped to reaffirm one’s professional study choice. Using qualitative description methods with purposeful sampling, data from interviews were collected, transcribed, analyzed and validated with a short questionnaire. The FOAL program, today, has several benefits for mentees, including partially helping in the preclinic to clinic transition and the increased insight into mentors’ clinical tasks and communication with patients. Informants described that FOAL also contributed positively to both mentee and mentor students’ learning motivation, collaborative skills and professional attitudes. Challenges were lack of organization/planning, not enough clinical hours, lack of clinical knowledge and persistent stress levels at the clinical transition. These issues are already being considered in the curriculum reform currently in progress and are also relevant to other dental curricula internationally.
A Stepwise Assessment of Parsimony and Fuzzy Entropy in Species Distribution Modelling
Authors: Estrada Alba , Real Raimundo
Published: August 2021

Entropy is intrinsic to the geographical distribution of a biological species. A species distribution with higher entropy involves more uncertainty, i.e., is more gradually constrained by the environment. Species distribution modelling tries to yield models with low uncertainty but normally has to reduce uncertainty by increasing their complexity, which is detrimental for another desirable property of the models, parsimony. By modelling the distribution of 18 vertebrate species in mainland Spain, we show that entropy may be computed along the forward-backwards stepwise selection of variables in Logistic Regression Models to check whether uncertainty is reduced at each step. In general, a reduction of entropy was produced asymptotically at each step of the model. This asymptote could be used to distinguish the entropy attributable to the species distribution from that attributable to model misspecification. We discussed the use of fuzzy entropy for this end because it produces results that are commensurable between species and study areas. Using a stepwise approach and fuzzy entropy may be helpful to counterbalance the uncertainty and the complexity of the models. The model yielded at the step with the lowest fuzzy entropy combines the reduction of uncertainty with parsimony, which results in high efficiency.
Low-Power Multiplexer Structures Targeting Efficient QCA Nanotechnology Circuit Designs
Authors: Almatrood Amjad , George Aby K. , Singh Harpreet
Published: August 2021

Quantum-dot cellular automata (QCA) technology is considered to be a possible alternative for circuit implementation in terms of energy efficiency, integration density and switching frequency. Multiplexer (MUX) can be considered to be a suitable candidate for designing QCA circuits. In this paper, two different structures of energy-efficient 2×1 MUX designs are proposed. These MUXes outperform the best existing design in terms of power consumption with approximate reductions of 26% and 35%. Moreover, similar or better performance factors such as area and latency are achieved compared to the available designs. These MUX structures can be used as fundamental energy-efficient building blocks for replacing the majority-based structures in QCA. The scalability property of the proposed MUXes is excellent and can be used for energy-efficient complex QCA circuit designs.
Analysis and Design for Output Voltage Regulation in Constant-on-Time-Controlled Fly-Buck Converter
Authors: Cho Younghoon , Jang Paul
Published: August 2021

Fly-buck converter is a multi-output converter with the structure of a synchronous buck converter structure on the primary side and a flyback converter structure on the secondary side, and can be utilized in various applications due to its many advantages. In terms of control, the primary side of the fly-buck converter has the same structure as a synchronous buck converter, allowing the constant-on-time (COT) control to be applied to the fly-buck converter. However, due to the inherent energy transfer principle, the primary-side output voltage regulation of COT controlled fly-buck converters may be poor, which can deteriorate the overall converter performance. Therefore, the primary output capacitor must be carefully designed to improve the voltage regulation characteristics. In this paper, a theoretical analysis of the output voltage regulation in COT controlled fly-buck converter is conducted, and based on this, a design guideline for the primary output capacitor considering the output voltage regulation is presented. The validity of the analysis and design guidelines was verified using a 5 W prototype of the COT controlled fly-buck converter for telecommunication auxiliary power supply.
Design Scalability Study of the Γ-Shaped Piezoelectric Harvester Based on Generalized Classical Ritz Method and Optimization
Authors: Jeong Sinwoo , Lee Soobum , Yoo Honghee
Published: August 2021

This paper studies the design scalability of a Γ-shaped piezoelectric energy harvester (ΓEH) using the generalized classical Ritz method (GCRM) and differential evolution algorithm. The generalized classical Ritz method (GCRM) is the advanced version of the classical Ritz method (CRM) that can handle a multibody system by assembling its equations of motion interconnected by the constraint equations. In this study, the GCRM is extended for analysis of the piezoelectric energy harvesters with material and/or orientation discontinuity between members. The electromechanical equations of motion are derived for the PE harvester using GCRM, and the accuracy of the numerical simulation is experimentally validated by comparing frequency response functions for voltage and power output. Then the GCRM is used in the power maximization design study that considers four different total masses—15 g, 30 g, 45 g, 60 g—to understand design scalability. The optimized ΓEH has the maximum normalized power density of 23.1 × 103 kg·s·m−3 which is the highest among the reviewed PE harvesters. We discuss how the design parameters need to be determined at different harvester scales.
Analyzing the Trade-Offs between Meeting Biorefinery Production Capacity and Feedstock Supply Cost: A Chance Constrained Approach
Authors: Lambert Lixia H. , DeVuyst Eric A. , English Burton C. , Holcomb Rodney
Published: August 2021

Commercial-scale switchgrass production for cellulosic biofuel remains absent in U.S. A well-recognized difficulty is the steady provision of high-quality feedstock to biorefineries. Switchgrass yield is random due to weather and growing conditions, with low yields during establishment years. Meeting biorefinery production capacity requirements 100% of the time or at any other frequency requires contracting sufficient amount of agricultural land areas to produce feedstock. Using chance-constrained programming, the trade-offs between the degree of certainty that refinery demand for feedstock and the cost of contracting production acreage is assessed. Varying the certainty from 60% to 95%, we find the costs of production, logistics and transportation ranged from 27% to 96% of the cost of 100% certainty. Investors and managers need to consider the cost of certainty of biomass acquisition when contracting for production acreage.