bluuebunny/crossref_metadata_2025 huggingface dataset:
Porous Coatings to Control Release Rates of Essential Oils to Generate an Atmosphere with Botanical Actives
Authors: Hettmann Kai , Monnard Fabien W. , Melo Rodriguez Gabriela , Hilty Florentine M. , Yildirim Selçuk , Schoelkopf Joachim
Published: March 2022

Essential oils have been used in diverse areas such as packaging, agriculture and cosmetics, for their antimicrobial and pesticide activity. The organic volatile compounds of the essential oils are involved in its activity. Controlling their release helps to prolong their functionality. In this study, a functionalized calcium carbonate porous coating was employed to control the release of thyme and rosemary oil in a confined space. The release rate was evaluated at 7 °C and 23 °C, gravimetrically. It was shown that the capillary effect of the porous coating slowed down the release of the volatiles into the headspace compared to the bulk essential oil. A linear drive force model was used to fit the obtained data from both essential oils. The model showed that rosemary reached the asymptotic mass loss equilibrium faster than thyme. This result can be explained by the diverse composition and concentration of monoterpenoids between the two essential oils. Temperature and degree of loading also played important roles in the desorption of the essential oils. It was observed that at high degrees of loading and temperatures the desorption of essential oils was higher. The above-described technology could be used for applications related to food preservation, pest control among others.
Progress in the Development of Graphene-Based Biomaterials for Tissue Engineering and Regeneration
Authors: Chen Chao , Xi Yuewei , Weng Yunxuan
Published: March 2022

Over the last few decades, tissue engineering has become an important technology for repairing and rebuilding damaged tissues and organs. The scaffold plays an important role and has become a hot pot in the field of tissue engineering. It has sufficient mechanical and biochemical properties and simulates the structure and function of natural tissue to promote the growth of cells inward. Therefore, graphene-based nanomaterials (GBNs), such as graphene and graphene oxide (GO), have attracted wide attention in the field of biomedical tissue engineering because of their unique structure, large specific surface area, good photo-thermal effect, pH response and broad-spectrum antibacterial properties. In this review, the structure and properties of typical GBNs are summarized, the progress made in the development of GBNs in soft tissue engineering (including skin, muscle, nerve and blood vessel) are highlighted, the challenges and prospects of the application of GBNs in soft tissue engineering have prospected.
Thermal and Mechanical Properties of Amorphous Silicon Carbide Thin Films Using the Femtosecond Pump-Probe Technique
Authors: Kim Yun Young
Published: March 2022

Nanoscale amorphous silicon carbide (a-SiC) thin films are widely used in engineering applications. It is important to obtain accurate information about their material properties because they often differ from those of the bulk state depending on the fabrication technique and process parameters. In this study, the thermal and mechanical properties of a-SiC thin films were evaluated using the femtosecond pump-probe technique, which provides high spatial and temporal resolutions sufficient to measure films that have a thickness of less than 300 nm. a-SiC films were grown using a plasma-enhanced chemical vapor deposition system, and the surface characteristics were analyzed using ellipsometry, atomic force microscopy, and X-ray reflectometry. The results show that the out-of-the-plane thermal conductivity of the films is lower than that of bulk crystalline SiC by two orders of magnitude, but the lower limit is dictated by the minimum thermal conductivity. In addition, a decrease in the mass density resulted in a reduced Young’s modulus by 13.6–78.4% compared to the literature values, implying low Si-C bond density in the microstructures. The scale effect on both thermal conductivity and Young’s modulus was not significant.
Polyester and Epoxy Resins with Increased Thermal Conductivity and Reduced Surface Resistivity for Applications in Explosion-Proof Enclosures of Electrical Devices
Authors: Szymiczek Małgorzata , Buła Dawid
Published: March 2022

Composite materials are still finding new applications that require the modification of various properties and are characterized by the summary impact on selected operational features. Due to the operating conditions of electrical equipment enclosures in potentially explosive atmospheres, the surface resistivity ensuring anti-electrostatic properties, i.e., below 109 Ω and resistance to the flame while maintaining appropriate operational enclosure properties is very important. It is also crucial to dissipate heat while reducing weight. Currently metal or cast-iron enclosures are used for various types of electrical devices. As part of the work, a material that can be used for a composite matrix for the enclosure was developed. The study aimed to assess the influence of selected fillers and chemical modifications on the thermal conductivity coefficient, resistivity, and strength properties of matrix materials for the production of electrical device enclosures used in the mining industry. Selected resins were modified with graphite, copper, and carbon black. Tests were carried out on the coefficient of thermal conductivity, surface resistivity, flammability, and flexural strength. At the final stage of the work, a multi-criteria analysis was carried out, which allowed the selection of a composite that meets the assumed characteristics to the highest degree. It is a vinyl ester composite modified with 15 wt.% MG394 and 5 wt.% MG1596 graphite (W2). The thermal conductivity of composite W2 is 5.64 W/mK, the surface resistivity is 5.2 × 103 Ω, the flexural strength is 50.61 MPa, and the flammability class is V0.
Biodegradable Mg–Zn–Ca-Based Metallic Glasses
Authors: Jin Chao , Liu Zhiyuan , Yu Wei , Qin Chunling , Yu Hui , Wang Zhifeng
Published: March 2022

Biodegradable Mg–Zn–Ca-based metallic glasses (MGs) present improved strength and superior corrosion resistance, compared to crystalline Mg. In particular, in vivo and in vitro attempts reveal that biodegradable Mg–Zn–Ca-based MGs possess excellent biocompatibility, suggesting that they are ideal candidates for temporary implant materials. However, the limited size and severe brittleness prevent their widespread commercialization. In this review, we firstly summarize the microstructure characteristic and mechanical properties of Mg–Zn–Ca-based MGs. Then, we provide a comprehensive and systematic understanding of the recent progress of the biocorrosion and biocompatibility of Mg–Zn–Ca-based MGs. Last, but not least, the outlook towards the fabrication routes, composition design, structure design, and reinforcement approaches of Mg–Zn–Ca-based MGs are briefly proposed.
“It Is Like a Feeling”: Theorizing Emotion in Mathematics through Complex Embodiment
Authors: Lambert Rachel , Hernández-Saca David , Mireles-Rios Rebeca , Monroy Castro Marilyn
Published: March 2022

Conversations of educational equity in mathematics necessitate a more deliberate, nuanced look at the mathematical processes of learning for students of color from historically marginalized communities. This paper describes the theoretical work of a research collaborative that seeks to develop understanding of the experiences around mathematical identity of Latinas labeled with Learning Disabilities in mathematics classrooms. Expanding the theory of Complex Embodiment from Disability Studies, we explore new interdisciplinary theoretical and methodological tools to analyze the emotional, embodied experience of learning mathematics in the social worlds of mathematics classrooms, using emotional discourse. We take up theoretical and methodological practices around intersectionality through analysis of how power and positioning operate in mathematics identity development. We find that the young woman whose narratives we explore in this paper is positioned through deficit discourses around disability and multilingual learners, yet she understands herself through a positive mathematical affinity she shares with her mother. Over time, we see her narratives shift emotionally away from mathematics, as well as away from this connection with her mother. Her narratives help us develop a theoretical perspective that understands emotion in mathematics learning as both embodied and socially constructed.
Non-Parametric Semi-Supervised Learning in Many-Body Hilbert Space with Rescaled Logarithmic Fidelity
Authors: Li Wei-Ming , Ran Shi-Ju
Published: March 2022

In quantum and quantum-inspired machine learning, a key step is to embed the data in the quantum space known as Hilbert space. Studying quantum kernel function, which defines the distances among the samples in the Hilbert space, belongs to the fundamental topics in this direction. In this work, we propose a tunable quantum-inspired kernel function (QIKF) named rescaled logarithmic fidelity (RLF) and a non-parametric algorithm for the semi-supervised learning in the quantum space. The rescaling takes advantage of the non-linearity of the kernel to tune the mutual distances of samples in the Hilbert space, and meanwhile avoids the exponentially-small fidelities between quantum many-qubit states. Being non-parametric excludes the possible effects from the variational parameters, and evidently demonstrates the properties of the kernel itself. Our results on the hand-written digits (MNIST dataset) and movie reviews (IMDb dataset) support the validity of our method, by comparing with the standard fidelity as the QIKF as well as several well-known non-parametric algorithms (naive Bayes classifiers, k-nearest neighbors, and spectral clustering). High accuracy is demonstrated, particularly for the unsupervised case with no labeled samples and the few-shot cases with small numbers of labeled samples. With the visualizations by t-stochastic neighbor embedding, our results imply that the machine learning in the Hilbert space complies with the principles of maximal coding rate reduction, where the low-dimensional data exhibit within-class compressibility, between-class discrimination, and overall diversity. The proposed QIKF and semi-supervised algorithm can be further combined with the parametric models such as tensor networks, quantum circuits, and quantum neural networks.
The Extremal Structures of r-Uniform Unicyclic Hypergraphs on the Signless Laplacian Estrada Index
Authors: Lu Hongyan , Zhu Zhongxun
Published: March 2022

SLEE has various applications in a large variety of problems. The signless Laplacian Estrada index of a hypergraph H is defined as SLEE(H)=∑i=1neλi(Q), where λ1(Q),λ2(Q),…,λn(Q) are the eigenvalues of the signless Laplacian matrix of H. In this paper, we characterize the unique r-uniform unicyclic hypergraphs with maximum and minimum SLEE.
Extended Half-Power Exponential Distribution with Applications to COVID-19 Data
Authors: Santoro Karol I. , Gómez Héctor J. , Barranco-Chamorro Inmaculada , Gómez Héctor W.
Published: March 2022

In this paper, the Extended Half-Power Exponential (EHPE) distribution is built on the basis of the Power Exponential model. The properties of the EHPE model are discussed: the cumulative distribution function, the hazard function, moments, and the skewness and kurtosis coefficients. Estimation is carried out by applying maximum likelihood (ML) methods. A Monte Carlo simulation study is carried out to assess the performance of ML estimates. To illustrate the usefulness and applicability of EHPE distribution, two real applications to COVID-19 data in Chile are discussed.
Rosuvastatin Induces Renal HO-1 Activity and Expression Levels as a Main Protective Mechanism against STZ-Induced Diabetic Nephropathy
Authors: Heeba Gehan H. , Ali Marwa A. M. , El-Sheikh Azza A. K.
Published: March 2022

Background and Objectives: Nephroprotective effect of statins is still controversial. The aim of this study was to investigate the possible hemin-like nephroprotective effect of rosuvastatin (RSV) in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: DN was induced in rats via a single dose of 50 mg/kg STZ i.p., with or without RSV (10 mg/kg orally) for 30 days. To investigate hemin-like effect of RSV on renal heme oxygenase-1 (HO-1), RSV was administered in the presence or absence of an inhibitor of HO-1; zinc protoporphyrin-XI (ZnPP), in a dose of 50 µmol/kg i.p. Results: Induction of diabetes with STZ caused, as expected, significant hyperglycemia, as well as deteriorated kidney function, lipid profile and histopathological architecture. The DN group also showed renal oxidative stress, indicated by decreased superoxide dismutase, catalase, and reduced glutathione, with increased malondialdehyde, myeloperoxidase and nitric oxide. Renal expression of inflammatory marker TNF-α, and pro-apoptotic marker caspase 3, were also increased in the DN group. Administration of RSV in DN rats did not improve glucose level but succeeded in recovering kidney function and normal structure as well as improving the lipid profile. RSV also improved renal oxidative, inflammatory, and apoptotic statuses. Interestingly, the administration of RSV increased renal expression and activity of HO-1 compared to the untreated DN group. Co-administration of ZnPP blocked the effect of RSV on HO-1 and deteriorated all RSV favorable effects. Conclusions: RSV can protect against DN, at least in part, via increasing renal HO-1 expression and/or activity, which seems to be upstream to RSV antioxidant, anti-inflammatory, and anti-apoptotic effects.
Long-Term Follow-Up of Survivors of Extracorporeal Life Support Therapy for Cardiogenic Shock: Are They Really Survivors?
Authors: Berger Rafal , Nemeth Attila , Sandoval Boburg Rodrigo , Vöhringer Louise , Lausberg Henning Florian , Acharya Metesh , Schlensak Christian , Popov Aron-Frederik
Published: March 2022

Background and Objectives: Cardiogenic shock (CS) is a medical emergency associated with a high mortality rate. Veno-arterial extracorporeal membrane oxygenation (VA-ECMO) has become an accepted therapy for CS. Despite widely available data for short-term survival rates, there are only limited data available regarding long-term outcomes following successful VA-ECMO therapy. Materials and Methods: We analyzed the demographics, past medical history, adverse events, and outcomes of survivors who received VA-ECMO support for CS at our center from January 2012 to December 2019. Post-cardiotomy cases were excluded. Results: A total of 578 VA-ECMO implantations on 564 consecutive patients due to CS were identified during the study period. Successful weaning was achieved in 207 (36.7%) patients. Among the survivors, 126 (63%) patients received VA-ECMO therapy without preceding cardiac surgery during their current admission. A follow-up exceeding 12 (mean: 36 ± 20.9) months was available in a total of 55 (43.7%) survivors. The mean VA-ECMO perfusion time was 10.9 (±7.7) days with a mean intensive care unit (ICU) stay of 38.2 (±29.9) days and a mean hospital stay of 49.9 (±30.5) days. A total of 3 deaths were recorded during long-term follow-up (mean survival of 26 ± 5.3 months). Conclusions: Despite the high mortality associated with VA-ECMO therapy, a long-term follow-up with an acceptably low rate of negative cardiac events can be achieved in many survivors. We observed an acceptable low rate of new cardiac events. Further evaluation, including a quality-of-life assessment and a close follow-up for rarer complications in these patients, is needed to elucidate the longer-term outcomes for survivors of invasive VA-ECMO therapy.
Corrosion Behavior of Gravity Cast and High-Pressure Die-Cast AM60 Mg Alloys with Ca and Y Addition
Authors: Yu Hui , Yang Xin , Yu Wei , Kim Youngmin , Kang Shaoming , Huang Lixin , You Bongsun , Liu Chunhai , Shin Kwangseon , Zhao Weimin
Published: March 2022

In this study, the microstructure and related corrosion behavior of AM60 alloys with/without Ca and Y addition upon gravity casting (GC) and high-pressure die-casting (HPDC) are investigated by means of SEM/EDS characterization, immersion/salt spray test, hydrogen releasing, as well as electrochemistry examination. When utilizing GC, AM60 alloy with Ca and Y addition (named AZXW6000 alloy) has better corrosion resistance compared with AM60 alloy. Thanks to grain refinement and second phase networks introduced in HPDC, the anti-corrosion properties of the AM alloy seem much better than those of the GC counterpart. The corrosion mechanism of both GCed and HPDCed AM60-based alloys is also investigated in detail. The inspiration from present work can provide more thinking for developing high corrosion-resistant Mg alloys using different casting methods.
Metabolites in Cherry Buds to Detect Winter Dormancy
Authors: Chmielewski Frank-M. , Götz Klaus-P.
Published: March 2022

Winter dormancy is still a “black box” in phenological models, because it evades simple observation. This study presents the first step in the identification of suitable metabolites which could indicate the timing and length of dormancy phases for the sweet cherry cultivar ‘Summit’. Global metabolite profiling detected 445 named metabolites in flower buds, which can be assigned to different substance groups such as amino acids, carbohydrates, phytohormones, lipids, nucleotides, peptides and some secondary metabolites. During the phases of endo- and ecodormancy, the energy metabolism in the form of glycolysis and the tricarboxylic acid (TCA) cycle was shut down to a minimum. However, the beginning of ontogenetic development was closely related to the up-regulation of the carbohydrate metabolism and thus to the generation of energy for the growth and development of the sweet cherry buds. From the 445 metabolites found in cherry buds, seven were selected which could be suitable markers for the ecodormancy phase, whose duration is limited by the date of endodormancy release (t1) and the beginning of ontogenetic development (t1*). With the exception of abscisic acid (ABA), which has been proven to control bud dormancy, all of these metabolites show nearly constant intensity during this phase.
Study of the Electron-Phonon Coupling in PbS/MnTe Quantum Dots Based on Temperature-Dependent Photoluminescence
Authors: Halim Nur Diyana , Zaini Muhammad Safwan , Talib Zainal Abidin , Liew Josephine Ying Chyi , Kamarudin Mazliana Ahmad
Published: March 2022

The temperature dependence of photoluminescence (PL) emission is a valuable tool for investigating carrier localization, recombination, and carrier–phonon interactions. Herein, electron–phonon couplings in lead sulfide (PbS) quantum dots (QDs) and lead sulfide/manganese tellurite (PbS/MnTe) QDs is reported. The effect of temperature on the PL emission of PbS and PbS/MnTe was explored within a temperature range of 10 to 300 K. When temperature increased, PL emission was blue-shifted due to the confinement effect. The gradual broadening of the full width at half maximum (FWHM) with increasing temperature indicates electron–phonon interactions. An analysis based on the Boson model revealed that the values of the exciton acoustic phonon coupling coefficient, σ, and temperature-dependent linewidth, γ, for PbS/MnTe were larger than those for PbS, indicating stronger exciton longitudinal-optical–phonon coupling in the compound structure.
Wintertime Simulations Induce Changes in the Structure, Diversity and Function of Antarctic Sea Ice-Associated Microbial Communities
Authors: Cono Violetta La , Smedile Francesco , Crisafi Francesca , Marturano Laura , Toshchakov Stepan V. , Spada Gina La , Bản Ninh Khắc , Yakimov Michail M.
Published: March 2022

Antarctic sea-ice is exposed to a wide range of environmental conditions during its annual existence; however, there is very little information describing the change in sea-ice-associated microbial communities (SIMCOs) during the changing seasons. It is well known that during the solar seasons, SIMCOs play an important role in the polar carbon-cycle, by increasing the total photosynthetic primary production of the South Ocean and participating in the remineralization of phosphates and nitrogen. What remains poorly understood is the dynamic of SIMCO populations and their ecological contribution to carbon and nutrient cycling throughout the entire annual life of Antarctic sea-ice, especially in winter. Sea ice at this time of the year is an extreme environment, characterized by complete darkness (which stops photosynthesis), extremely low temperatures in its upper horizons (down to −45 °C) and high salinity (up to 150–250 psu) in its brine inclusions, where SIMCOs thrive. Without a permanent station, wintering expeditions in Antarctica are technically difficult; therefore, in this study, the process of autumn freezing was modelled under laboratory conditions, and the resulting ‘young ice’ was further incubated in cold and darkness for one month. The ice formation experiment was primarily designed to reproduce two critical conditions: (i) total darkness, causing the photosynthesis to cease, and (ii) the presence of a large amount of algae-derived organic matter. As expected, in the absence of photosynthesis, the activity of aerobic heterotrophs quickly created micro-oxic conditions, which caused the emergence of new players, namely facultative anaerobic and anaerobic microorganisms. Following this finding, we can state that Antarctic pack-ice and its surrounding ambient (under-ice seawater and platelet ice) are likely to be very dynamic and can quickly respond to environmental changes caused by the seasonal fluctuations. Given the size of Antarctic pack-ice, even in complete darkness and cessation of photosynthesis, its ecosystem appears to remain active, continuing to participate in global carbon-and-sulfur cycling under harsh conditions.
Selection of Lactiplantibacillus Strains for the Production of Fermented Table Olives
Authors: Zotta Teresa , Giavalisco Marilisa , Parente Eugenio , Picariello Gianluca , Siano Francesco , Ricciardi Annamaria
Published: March 2022

Lactiplantibacillus strains (n. 77) were screened for technological properties (e.g., xylose fermentation, EPS production, antimicrobial activity, tolerance to NaCl and phenolic compounds, oleuropein degradation and hydroxytyrosol formation) relevant for the production of fermented table olives. Survival to olive mill wastewater (OMW) and to simulated gastro-intestinal tract (GIT), the capability to grow at different combinations of NaCl and pH values, radical scavenging activities and biofilm formation were further investigated in 15 selected strains. The screening step revealed high diversity among Lactiplantibacillus strains. Most of the strains were able to ferment xylose, while only a few strains produced EPS and had inhibitory activity against Y. lipolytica. Resistance to phenolic compounds (gallic, protocatechuic, hydroxybenzoic and syringic acids), as well as the ability to release hydroxytyrosol from oleuropein, was strain-specific. OMWs impaired the survival of selected strains, while combinations of NaCl ≤ 6% and pH ≥ 4.0 were well tolerated. DPPH and hydroxyl radical degradation were strain-dependent, while the capability to form biofilm was affected by incubation time. Strains were very tolerant to the GIT. The genome of Lpb. pentosus O17 was sequenced and analysed to verify the presence of genes involved in the degradation and metabolism of phenolic compounds. O17 lacks carboxylesterase and gallate decarboxylase (subunits B and D) sequences, and its gene profile differs from that of other publicly available Lpb. pentosus genomes.
Recovery of Platinum Group Metals from Leach Solutions of Spent Catalytic Converters Using Custom-Made Resins
Authors: Moleko-Boyce Pulleng , Makelane Hlamulo , Ngayeka Mbokazi Z. , Tshentu Zenixole R.
Published: March 2022

Platinum group metals (PGMs) play a key role in modern society as they find application in clean technologies and other high-tech equipment. Spent catalytic converters as a secondary resource contain higher PGM concentrations and the recovery of these metals via leaching is continuously being improved but efforts are also directed at the purification of individual metal ions. The study presents the recovery of PGMs, namely, rhodium (Rh), platinum (Pt) and palladium (Pd) as well as base metals, namely, zinc (Zn), nickel (Ni), iron (Fe), manganese (Mn) and chromium (Cr) using leachates from spent diesel and petrol catalytic converters. The largest amount of Pt was leached from the diesel catalytic converter while the petrol gave the highest amount of Pd when leached with aqua regia. Merrifield beads (M) were functionalized with triethylenetetramine (TETA), ethane-1,2-dithiol (SS) and bis((1H-benzimidazol-2-yl)methyl)sulfide (NSN) to form M-TETA, M-SS and M-NSN, respectively, for recovery of PGMs and base metals from the leach solutions. The adsorption and loading capacities of the PGMs and base metals were investigated using column studies at 1 M HCl concentration. The loading capacity was observed in the increasing order of Pd to be 64.93 mmol/g (M-SS), 177.07 mmol/g (M-NSN), and 192.0 mmol/g (M-TETA), respectively, from a petrol catalytic converter. The M-NSN beads also had a much higher loading capacity for Fe (489.55 mmol/g) compared to other base metals. The finding showed that functionalized Merrifield resins were effective for the simultaneous recovery of PGMs and base metals from spent catalytic converters.
Therapeutic Potential of Highly Selective A3 Adenosine Receptor Ligands in the Central and Peripheral Nervous System
Authors: Coppi Elisabetta , Cherchi Federica , Venturini Martina , Lucarini Elena , Corradetti Renato , Di Cesare Mannelli Lorenzo , Ghelardini Carla , Pedata Felicita , Pugliese Anna
Published: March 2022

Ligands of the Gi protein-coupled adenosine A3 receptor (A3R) are receiving increasing interest as attractive therapeutic tools for the treatment of a number of pathological conditions of the central and peripheral nervous systems (CNS and PNS, respectively). Their safe pharmacological profiles emerging from clinical trials on different pathologies (e.g., rheumatoid arthritis, psoriasis and fatty liver diseases) confer a realistic translational potential to these compounds, thus encouraging the investigation of highly selective agonists and antagonists of A3R. The present review summarizes information on the effect of latest-generation A3R ligands, not yet available in commerce, obtained by using different in vitro and in vivo models of various PNS- or CNS-related disorders. This review places particular focus on brain ischemia insults and colitis, where the prototypical A3R agonist, Cl-IB-MECA, and antagonist, MRS1523, have been used in research studies as reference compounds to explore the effects of latest-generation ligands on this receptor. The advantages and weaknesses of these compounds in terms of therapeutic potential are discussed.
Arene Ru(II) Complexes with Difluorinated Ligands Act as Potential Inducers of S-Phase Arrest via the Stabilization of c-myc G-Quadruplex DNA
Authors: Zeng Liang , Yuan Chanling , Shu Jing , Qian Jiayi , Wu Qiong , Chen Yanhua , Wu Ruzhen , Ouyang Xiaoming , Li Yuan , Mei Wenjie
Published: March 2022

Here, a series of half-sandwich arene Ru(II) complexes with difluorinated ligands [Ru(η6-arene)(L)Cl] (L1 = 2-(2,3-difluorophenyl)imidazole[4,5f][1,10]-phenanthroline; L2 = 2-(2,4-difluorophenyl)imidazole[4,5f][1,10]-phenanthroline; arene = benzene, toluene, and p-cymene) were synthesized and characterized. Molecular docking analysis showed that these complexes bind to c-myc G-quadruplex DNA through either groove binding or π–π stacking, and the relative difluorinated site in the main ligand plays a role in regulating the binding mode. The binding behavior of these complexes with c-myc G-quadruplex DNA was evaluated using ultraviolet–visible spectroscopy, fluorescence intercalator displacement assay, fluorescence resonance energy transfer melting assay, and polymerase chain reaction. The comprehensive analysis indicated that complex 1 exhibited a better affinity and stability in relation to c-myc G-quadruplex DNA with a DC50 of 6.6 μM and ΔTm values of 13.09 °C, than other molecules. Further activity evaluation results displayed that this class of complexes can also inhibit the growth of various tumor cells, especially complexes 3 and 6, which exhibited a better inhibitory effect against human U87 glioblastoma cells (51.61 and 23.75 μM) than other complexes, even superior to cisplatin (32.59 μM). Owing to a befitting lipophilicity associated with the high intake of drugs by tumor cells, complexes 3 and 6 had favorable lipid-water partition coefficients of −0.6615 and −0.8077, respectively. Moreover, it was found that complex 6 suppressed the proliferation of U87 cells mainly through an induced obvious S phase arrest and slight apoptosis, which may have resulted from the stabilization of c-myc G-quadruplex DNA to block the transcription and expression of c-myc. In brief, these types of arene Ru(II) complexes with difluorinated ligands can be developed as potential inducers of S-phase arrest and apoptosis through the binding and stabilization of c-myc G-quadruplex DNA, and could be used in clinical applications in the future.
Inhibition of Filamentous Thermosensitive Mutant-Z Protein in Bacillus subtilis by Cyanobacterial Bioactive Compounds
Authors: Gurnani Manisha , Rath Prangya , Chauhan Abhishek , Ranjan Anuj , Ghosh Arabinda , Lal Rup , Mukerjee Nobendu , Aljarba Nada H. , Alkahtani Saad , Rajput Vishnu D. , Sushkova Svetlana , Prazdnova Evgenya V. , Minkina Tatiana , Jindal Tanu
Published: March 2022

Antibiotic resistance is one of the major growing concerns for public health. Conventional antibiotics act on a few predefined targets and, with time, several bacteria have developed resistance against a large number of antibiotics. The WHO has suggested that antibiotic resistance is at a crisis stage and identification of new antibiotics and targets could be the only approach to bridge the gap. Filamentous Temperature Sensitive-Mutant Z (Fts-Z) is one of the promising and less explored antibiotic targets. It is a highly conserved protein and plays a key role in bacterial cell division by introducing a cytokinetic Z-ring formation. In the present article, the potential of over 165 cyanobacterial compounds with reported antibiotic activity against the catalytic core domain in the Fts-Z protein of the Bacillus subtilis was studied. The identified cyanobacterial compounds were screened using the GLIDE module of Maestro v-2019-2 followed by 100-ns molecular dynamics (MD) simulation. Ranking of the potential compound was performed using dock score and MMGBSA based free energy. The study reported that the docking score of aphanorphine (−6.010 Kcalmol−1) and alpha-dimorphecolic acid (ADMA) (−6.574 Kcalmol−1) showed significant role with respect to the reported potential inhibitor PC190723 (−4.135 Kcalmol−1). A 100 ns MD simulation infers that Fts-Z ADMA complex has a stable conformation throughout the progress of the simulation. Both the compounds, i.e., ADMA and Aphanorphine, were further considered for In-vitro validation by performing anti-bacterial studies against B. subtilis by agar well diffusion method. The results obtained through In-vitro studies confirm that ADMA, a small molecule of cyanobacterial origin, is a potential compound with an antibacterial activity that may act by inhibiting the novel target Fts-Z and could be a great drug candidate for antibiotic development.
Copper-Free Halodediazoniation of Arenediazonium Tetrafluoroborates in Deep Eutectic Solvents-like Mixtures
Authors: Ghigo Giovanni , Bonomo Matteo , Antenucci Achille , Reviglio Chiara , Dughera Stefano
Published: March 2022

Deep Eutectic Solvent (DES)-like mixtures, based on glycerol and different halide organic and inorganic salts, are successfully exploited as new media in copper-free halodediazoniation of arenediazonium salts. The reactions are carried out in absence of metal-based catalysts, at room temperature and in a short time. Pure target products are obtained without the need for chromatographic separation. The solvents are fully characterized, and a computational study is presented aiming to understand the reaction mechanism.
First Study on the Electronic and Donor Atom Properties of the Ultra-Thin Nanoflakes Quantum Dots
Authors: Belamkadem Laaziz , Mommadi Omar , Boussetta Reda , Chnafi Mohamed , Vinasco Juán A. , Laroze David , Pérez Laura M. , El Moussaouy Abdelaziz , Meziani Yahya M. , Kasapoglu Esin , Tulupenko Viktor , Duque Carlos A.
Published: March 2022

Nanoflakes ultra-thin quantum dots are theoretically studied as innovative nanomaterials delivering outstanding results in various high fields. In this work, we investigated the surface properties of an electron confined in spherical ultra-thin quantum dots in the presence of an on-center or off-center donor impurity. Thus, we have developed a novel model that leads us to investigate the different nanoflake geometries by changing the spherical nanoflake coordinates (R, α, ϕ). Under the infinite confinement potential model, the study of these nanostructures is performed within the effective mass and parabolic band approximations. The resolution of the Schrödinger equation is accomplished by the finite difference method, which allows obtaining the eigenvalues and wave functions for an electron confined in the nanoflakes surface. Through the donor and electron energies, the transport, optoelectronic, and surface properties of the nanostructures were fully discussed according to their practical significance. Our findings demonstrated that these energies are more significant in the small nanoflakes area by altering the radius and the polar and azimuthal angles. The important finding shows that the ground state binding energy depends strongly on the geometry of the nanoflakes, despite having the same surface. Another interesting result is that the presence of the off-center shallow donor impurity permits controlling the binding energy, which leads to adjusting the immense behavior of the curved surface nanostructures.
Impact of COVID-19 Lockdown on Anthropometric Variables, Blood Pressure, and Glucose and Lipid Profile in Healthy Adults: A before and after Pandemic Lockdown Longitudinal Study
Authors: Ramírez Manent José Ignacio , Altisench Jané Bárbara , Sanchís Cortés Pilar , Busquets-Cortés Carla , Arroyo Bote Sebastiana , Masmiquel Comas Luis , López González Ángel Arturo
Published: March 2022

In December 2019, 27 cases of pneumonia were reported in Wuhan. In 2020, the causative agent was identified as a virus called SARS-CoV-2. The disease was called “coronavirus disease 2019” (COVID-19) and was determined as a Public Health Emergency. The main measures taken to cope with this included a state of lockdown. The aim of this study was to assess how the unhealthy lifestyles that ensued influenced different parameters. A prospective study was carried out on 6236 workers in a Spanish population between March 2019 and March 2021. Anthropometric, clinical, and analytical measurements were performed, revealing differences in the mean values of anthropometric and clinical parameters before and after lockdown due to the pandemic, namely increased body weight (41.1 ± 9.9–43.1 ± 9.9), BMI (25.1 ± 4.7–25.9 ± 4.7), and percentage of body fat (24.5 ± 9.1–26.9 ± 8.8); higher total cholesterol levels, with a statistically significant increase in LDL levels and a reduction in HDL; and worse glucose levels (90.5 ± 16.4–95.4 ± 15.8). Lockdown can be concluded to have had a negative effect on health parameters in both sexes in all age ranges, causing a worsening of cardiovascular risk factors.
The Effectiveness of Psychoeducational Interventions in Adolescents’ Anxiety: A Systematic Review Protocol
Authors: Morgado Tânia , Lopes Vera , Carvalho Dulce , Santos Eduardo
Published: March 2022

The COVID-19 pandemic had a strong impact on increasing anxiety in adolescents. This systematic review aims to identify the most effective psychoeducational interventions for reducing anxiety in adolescents following the guidelines of the Joanna Briggs Institute (JBI). The inclusion and exclusion criteria have been defined, and the search strategy has been planned. The search strategy will aim to locate both published and unpublished studies using, among other databases: CINAHL Plus with Full Text; PubMed; the Cochrane Central Register of Controlled Trials; and the JBI Database of Systematic Reviews. Following the search, all identified citations will be collated and uploaded into Endnote, and duplicates removed. Titles and abstracts will then be screened by two independent reviewers and by a third reviewer if a disagreement occurs. The results of the search will be reported in full in the final systematic review and presented in the PRISMA flow diagram. Eligible studies will be critically appraised for methodological quality using standardized critical appraisal instruments from the JBI. Data will be extracted from the studies included using the standardized JBI data extraction tool. For data synthesis, studies will be pooled using JBI SUMARI. The GRADE approach for grading the certainty of evidence will be followed, and a summary of findings will be created using GRADEPro GDT software. The results from this systematic review are expected to provide an overview of the most effective psychoeducational interventions for reducing anxiety in adolescents, allowing researchers to design and propose a new multicomponent psychoeducational intervention that will be validated and tested in the future. PROSPERO protocol registration number: CRD42020204356.
Pseudomonas syringae on Plants in Iceland Has Likely Evolved for Several Million Years Outside the Reach of Processes That Mix This Bacterial Complex across Earth’s Temperate Zones
Authors: Morris Cindy E. , Ramirez Natalia , Berge Odile , Lacroix Christelle , Monteil Cécile , Chandeysson Charlotte , Guilbaud Caroline , Blischke Anett , Sigurbjörnsdóttir Margrét Auður , Vilhelmsson Oddur Þ.
Published: March 2022

Here we report, for the first time, the occurrence of the bacteria from the species complex Pseudomonas syringae in Iceland. We isolated this bacterium from 35 of the 38 samples of angiosperms, moss, ferns and leaf litter collected across the island from five habitat categories (boreal heath, forest, subalpine and glacial scrub, grazed pasture, lava field). The culturable populations of P. syringae on these plants varied in size across 6 orders of magnitude, were as dense as 107 cfu g−1 and were composed of strains in phylogroups 1, 2, 4, 6, 7, 10 and 13. P. syringae densities were significantly greatest on monocots compared to those on dicots and mosses and were about two orders of magnitude greater in grazed pastures compared to all other habitats. The phylogenetic diversity of 609 strains of P. syringae from Iceland was compared to that of 933 reference strains of P. syringae from crops and environmental reservoirs collected from 27 other countries based on a 343 bp sequence of the citrate synthase (cts) housekeeping gene. Whereas there were examples of identical cts sequences across multiple countries and continents among the reference strains indicating mixing among these countries and continents, the Icelandic strains grouped into monophyletic lineages that were unique compared to all of the reference strains. Based on estimates of the time of divergence of the Icelandic genetic lineages of P. syringae, the geological, botanical and land use history of Iceland, and atmospheric circulation patterns, we propose scenarios whereby it would be feasible for P. syringae to have evolved outside the reach of processes that tend to mix this bacterial complex across the planet elsewhere.
Pharmacokinetic Evaluation of a Novel Transdermal Ketoprofen Formulation in Healthy Dogs
Authors: Ravuri Halley Gora , Satake Nana , Balmanno Alexandra , Skinner Jazmine , Kempster Samantha , Mills Paul C.
Published: March 2022

Dogs undergo various surgical procedures such as castration, ovariohysterectomy, and other orthopedic procedures, which are known to cause inflammation and pain. Non-steroidal anti-inflammatory drugs (NSAIDs) are very effective analgesics for alleviating postoperative pain in veterinary medicine. Ketoprofen is currently approved in Australia and the United States for treating different painful conditions in dogs. This study evaluated the pharmacokinetic parameters of ketoprofen after intravenous (IV) and transdermal (TD) administration in healthy dogs. A novel transdermal ketoprofen (TDK) formulation containing 20% ketoprofen, dissolved in a combination of 45:45% isopropanol and Transcutol, along with 10% eucalyptus oil, was developed and evaluated for in vitro dermal permeation using Franz diffusion cells. A crossover study was then conducted to determine the pharmacokinetic parameters of the formulation in six dogs following IV ketoprofen (1 mg/kg) and TDK (10 mg/kg) administration. A liquid chromatography–mass spectrometry (LC-M/MS) method was used to measure plasma concentrations of ketoprofen over time, and a non-compartmental analysis determined the pharmacokinetic parameters. The mean terminal elimination half-life (T½ h), AUC0-t (µg·h/mL), and mean residence time (MRT, h) between IV and TDK groups were 4.69 ± 1.33 and 25.77 ± 22.15 h, 15.75 ± 7.72 and 8.13 ± 4.28 µg·h/mL, and 4.86 ± 1.81 and 41.63 ± 32.33 h, respectively. The calculated bioavailability (F%) was ~7%, with a lag time of 30 min to achieve effective plasma concentrations after the application of TDK.
An Insight into Salvia haematodes L. (Lamiaceae) Bioactive Extracts Obtained by Traditional and Green Extraction Procedures
Authors: Tundis Rosa , Passalacqua Nicodemo Giuseppe , Bonesi Marco , Loizzo Monica Rosa
Published: March 2022

Even though Salvia is one of the most known genera of the Lamiaceae family, some traditionally used Salvia species are still now less investigated. To that end, the present study aims to evaluate the chemical profile and the potential bioactivities of extracts and related fractions obtained from the endemic sage Salvia haematodes L. by applying a traditional extraction method such as Soxhlet apparatus (SHS) and the rapid solid–liquid dynamic extraction (RSLDE) by Naviglio extractor® (SHN), considered among the “green techniques” operating at room temperature and with minimum solvent employment and minimum energy. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity was measured by a modified Ellman’s method. The antioxidant activity was investigated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing ability power (FRAP), and β-carotene bleaching tests. The SHN methanol fraction resulted the most active in all assays in particular in inhibiting lipid peroxidation with IC50 of 1.7 and 1.6 μg/mL, respectively, after 30 and 60 min of incubation. The SHN n-hexane fraction exhibited a selective activity against AChE with half-maximal inhibitory concentration (IC50) of 22.9 μg/mL, while the SHS n-hexane extract was more active against BChE (IC50 of 30.9 μg/mL). Based on these results, these fractions were subjected to further bio-fractionation by Medium Pressure Liquid Chromatography (MPLC) and the relative obtained fractions were investigated for their AChE and BChE inhibitory activity. A comparative analysis with bio-activity and chemical profile was performed. The observed biological effects provided us with a good starting point for further studies on S. haematodes extracts and fractions such as agents beneficial for the treatment of AD.
Jacaranone Derivatives with Antiproliferative Activity from Crepis pulchra and Relevance of This Group of Plant Metabolites
Authors: Dávid Csilla Zsuzsanna , Kúsz Norbert , Pinke Gyula , Kulmány Ágnes , Zupkó István , Hohmann Judit , Vasas Andrea
Published: March 2022

Jacaranones are a small group of specific plant metabolites with promising biological activities. The occurrence of jacaranones is limited to only a few plant families, with Asteraceae being the most abundant source of these compounds. Therefore, jacaranones can also serve as chemotaxonomic markers. Our phytochemical investigation of Crepis pulchra L. (Asteraceae) resulted in three jacaranone derivatives (jacaranone, 2,3-dihydro-2-hydroxyjacaranone, 2,3-dihydro-2-methoxyjacaranone), and (6R,9S)-3-oxo-α-ionol-β-d-glucopyranoside, fulgidic acid, 12,15-octadecadienoic acid methyl ester, scopoletin and apigenin-7-O-β-d-glucoside. This is the first report on the isolation of jacaranones from a species belonging to the Cichorioideae subfamily of Asteraceae. Jacaranone derivatives were subjected to an in vitro antiproliferative assay against a panel of human cancer cell lines (MCF-7, MDA-MB-231, HeLa, and C33A), revealing high or moderate activities, with IC50 values ranging from 6.3 to 26.5 μM.
Use of Wood in Additive Manufacturing: Review and Future Prospects
Authors: Krapež Tomec Daša , Kariž Mirko
Published: March 2022

Polymers filled with natural-based fillers have shown growing demand/interest in recent years, including in additive manufacturing. Like most natural fillers in 3D printing, wood particles serve mainly as a filler that lowers the cost of the printing material due to their low price. However, could wood be used as a main ingredient to affect/improve the properties of 3D-printed parts? Several advantages, such as its reinforcing ability, biodegradability, availability as waste material from other industries, ability to be used in different forms or only in partial components, recycling options or even the use of its undesirable hydromorph-induced dimensional instability for 4D printing, indicate the importance of exploring its use in 3D printing. A review of publications on 3D printing with wood biomass and technologies involving the use of wood particles and components was conducted to identify the possibilities of using wood in additive technologies and their potential.
Assessment of the Possibilities for Removal of Ni (II) from Contaminated Water by Activated Carbon foam Derived from Treatment Products of RDF
Authors: Stoycheva Ivanka , Tsyntsarski Boyko , Kosateva Angelina , Petrova Bilyana , Dolashka Pavlinka
Published: March 2022

Carbon foam is a sophisticated porous material with wide applications that depend on its structure, low density, thermal conductivity and electrical characteristics. This study deals with the preparation of carbon foam by the thermo-oxidative modification with HNO3 of mixtures containing different organic materials with appropriate chemical characteristics—furfural and tar pitch derived from RDF. Carbon foam is characterized by thermogravimetry, differential scanning calorimetry, elemental analysis, Raman spectroscopy, N2 sorption, infrared spectroscopy and scanning electron spectroscopy. The investigation of adsorption activity of carbon foam towards nickel (II) in water solution is carried out. Experimental results fit very well with the Langmuir adsorption model. The carbon foam, obtained from tar pitch derived from RDF and furfural, shows a high adsorption capacity towards nickel ions (203.67 mg/g). The high adsorption capacity could be explained by the properties of the adsorbent—moderately high surface area, micro-mesoporous texture and presence of oxygen-containing surface groups.