bluuebunny/crossref_metadata_2025 huggingface dataset:
Contribution to Active Thermal Protection Research—Part 1 Analysis of Energy Functions by Parametric Study
Authors: Mučková Veronika , Kalús Daniel , Koudelková Daniela , Kurčová Mária , Straková Zuzana , Sokol Martin , Ingeli Rastislav , Šťastný Patrik , Janík Peter
Published: May 2023

The thermal energy consumption for heating objects is one of the main parameters deciding their technical and structural solution. Reducing energy consumption is realized in various ways. The most used method is passive thermal protection, that is, increasing the thermal insulation parameters of the envelope constructions and optimizing the operation of the technical equipment of the buildings. There are also methods of active thermal protection to reduce heat leakage through non-transparent parts of the building envelope. Active thermal protection (ATP) is a dynamic process that applies primarily to building structures with integrated energetically active elements that fulfill one or more functions in mutually exclusive operating modes of energy systems and heat sources, such as thermal barriers (TB), large-scale heating/cooling, long-term heat/cool storage, capturing solar energy, and energy from the surroundings or even recovering heat in the cold season or cold in the warm season and other combinations. The research area focuses on analyzing active thermal protection’s energy potential. The methodology is based on a parametric study of dynamic thermal resistance (DTR), and heat flows to the interior and exterior from ATP for the investigated envelope of the experimental house EB2020 made of aerated concrete blocks. Such constructions generally show a consistently high DTR of 11.8 to 30.8 ((m2·K)/W) at a low mean heat transfer temperature of 10 to 16 °C, corresponding to the thermal insulation of 300 to 1000 mm. In the case of ATP storage heating with an average temperature of the heat-carrying substance of 30 °C, the heat flow into the interior of the investigated structure would be only 2.31 W/m2. Therefore, they are only suitable for a thermal barrier and heat/cold accumulation. Based on the synthesis and induction of analogical forms of the results of previous research into recommendations for the design of individual energy functions of ATP for perimeter structures of buildings, we present heat flows to the interior/exterior when changing the material of the layers towards the interior from ATP and describe possible variants of the development of building structures with integrated energetically active elements.
Agricultural Plant Residues as Potential Co-Substrates for Biogas Production
Authors: Frankowski Jakub , Czekała Wojciech
Published: May 2023

Plant biomass can be used in many directions for bioenergy production. Biogas can be produced from a most diverse group of substrates compared to liquid or solid biofuels. The choice of substrates and technologies is crucial because it will allow getting the expected results. Not without significance is also the price and availability of substrates. Therefore, waste and residues are increasingly being used. Accordingly, the aim of the review was to analyze the potential of biogas production from agricultural plant residues and the effectiveness of using this feedstock as a co-substrate in anaerobic digestion. In this article, selected agricultural plant residues are collected, and their advantages and disadvantages as substrates for biogas production are described. Moreover, the effective technology of biogas production by anaerobic digestion on an industrial scale and calculations to obtain biogas and methane efficiency of the substrates are also included. In addition, the summarized biogas efficiency of selected plant agricultural waste under mesophilic conditions studied by many researchers is shown. On the basis of the analyzed results of this research, it can be concluded that agricultural plant residues have great potential as co-substrates for biogas production. It is important to experimentally determine both the biogas and the methane efficiency of the substrate, representing a potential raw material for the production of gaseous biofuels. The use of artificial neural networks in the prediction of biogas emission is future-proof and should facilitate the management of biogas plants. The use of waste from the cultivation and processing of plant raw materials will not only help to manage this waste rationally, but also contribute to the increase in production of renewable energy sources. Accordingly, the circular economy in terms of the management of agricultural plant residues to produce biogas will have a multi-faceted, positive impact on the environment. On the basis of this review, it can be concluded that numerous agricultural plant residues can be used as potential co-substrates for biogas production.
DC-DC Boost Converter with Reduced Switching Losses in Wide Range of Voltage Gain
Authors: Mondzik Andrzej , Piróg Stanisław , Stala Robert , Penczek Adam , Gucwa Piotr
Published: May 2023

This article presents the results of research on a new topology of a DC-DC converter in which switching losses are reduced by an auxiliary switching circuit (ASC). The proposed ASC can work as a passive or active circuit depending on the voltage gain of the converter. In both cases, this does not affect the operation of the main circuit and is analogous to a classic boost converter, which allows the use of the same control circuits and methods. The operation of the ASC is synchronized with the control of the main converter transistor. The reduction of transistor turn-off losses over a wide range of converter gain is a unique advantage of the proposed auxiliary circuit. This is evidenced by the comparison of the loss reduction with the classic boost converter and the reference converter with soft turn off. This article presents an in-depth analytical discussion related to the reduction of power losses, supported by simulation results, and verified experimentally.
From Microalgae to Bioenergy: Recent Advances in Biochemical Conversion Processes
Authors: Parakh Sheetal Kishor , Tian Zinong , Wong Jonathan Zhi En , Tong Yen Wah
Published: May 2023

Concerns about rising energy demand, fossil fuel depletion, and global warming have increased interest in developing and utilizing alternate renewable energy sources. Among the available renewable resources, microalgae biomass, a third-generation feedstock, is promising for energy production due to its rich biochemical composition, metabolic elasticity, and ability to produce numerous bioenergy products, including biomethane, biohydrogen, and bioethanol. However, the true potential of microalgae biomass in the future bioenergy economy is yet to be realized. This review provides a comprehensive overview of various biochemical conversion processes (anaerobic digestion, direct biophotolysis, indirect biophotolysis, photo fermentation, dark fermentation, microalgae-catalyzed photo fermentation, microalgae-catalyzed dark fermentation, and traditional alcoholic fermentation by ethanologenic microorganisms) that could be adapted to transform microalgae biomass into different bioenergy products. Recent advances in biochemical conversion processes are compiled and critically analyzed, and their limitations in terms of process viability, efficacy, scalability, and economic and environmental sustainability are highlighted. Based on the current research stage and technological development, biomethane production from anaerobic digestion and bioethanol production from traditional fermentation are identified as promising methods for the future commercialization of microalgae-based bioenergy. However, significant challenges to these technologies’ commercialization remain, including the high microalgae production costs and low energy recovery efficiency. Future research should focus on reducing microalgae production costs, developing an integrated biorefinery approach, and effectively utilizing artificial intelligence tools for process optimization and scale-up to solve the current challenges and accelerate the development of microalgae-based bioenergy.
Effect of CO2 Laser Microperforation Pretreatment on the Dehydration of Apple Slices during Refractive Window Drying
Authors: Núñez Helena , Jaques Aldonza , Belmonte Karyn , Córdova Andrés , Lafuente German , Ramírez Cristian
Published: May 2023

This research studied the use of CO2 LASER microperforation as a pretreatment for the refractive window (RW) drying of apple slices with respect to total polyphenol content (TPC), antioxidant capacity, color ΔE, and product stability under accelerated storage. For this purpose, the processing variables assessed were pore size (200–600 µm), pore density (9–25 pores/cm2), and drying temperature (70–90 °C). As baseline criteria, a comparison with respect to the control without microperforations and samples subjected to conventional tunnel and lyophilization were also considered. The increase in the pore size from 200 to 600 µm resulted in shorter drying times (≤40 min), minimal change in color (ΔE) and loss of TPC, while DPPH was negatively affected by the combined effect of the pore density and the drying temperature. In general, the use of RW with CO2 resulted in apples of higher quality than those obtained in conventional drying and comparable to those obtained through the use of freeze-drying. Finally, during accelerated storage, quality attributes decreased significantly for samples dried at 90 °C regardless of whether microperforations were used, suggesting that a compromise between drying temperature and pore size must be weighed to reduce processing time and to avoid further quality losses during storage.
Changes Induced by Heat Moisture Treatment in Wheat Flour and Pasta Rheological, Physical and Starch Digestibility Properties
Authors: Ungureanu-Iuga Mădălina , Mironeasa Silvia
Published: May 2023

Wheat is one of the main crops that is cultivated and consumed in the world. Since durum wheat is less abundant and more expensive than other types, pasta producers can use common wheat by applying various techniques to achieve the desired quality. A heat moisture treatment was applied to common wheat flour, and the effects on dough rheology and texture, and pasta cooking quality, color, texture, and resistant starch content were evaluated. The results revealed that heat moisture treatment temperature and moisture content induced a proportional increase in visco-elastic moduli, dough firmness, pasta cooking solids loss, and luminosity, as they were higher compared to the control. The breaking force of uncooked pasta decreased when the flour moisture content increased, while the opposite trend was observed for resistant starch content. The highest resistant starch values were obtained for the samples treated at the lowest temperature (60 °C). Significant correlations (p < 0.05) were obtained between some of the textural and physical characteristics analyzed. The studied samples can be grouped in three clusters characterized by different properties. Heat moisture treatment is a convenient physical modification of starch and flours that can be employed in the pasta industry. These results underline the opportunity to enhance common pasta processing and final product functionality by using a green and non-toxic technique to develop new functional products.
Methylation Levels in the Promoter Region of FHIT and PIAS1 Genes Associated with Mastitis Resistance in Xinjiang Brown Cattle
Authors: Zhong Liwei , Ma Shengchao , Wang Dan , Zhang Menghua , Tian Yuezhen , He Junmin , Zhang Xiaoxue , Xu Lei , Wu Cuiling , Dong Mingming , Gou Murong , Huang Xixia , Tian Kechuan
Published: May 2023

Mastitis causes serious economic losses in the dairy industry, but there are no effective treatments or preventive measures. In this study, the ZRANB3, PIAS1, ACTR3, LPCAT2, MGAT5, and SLC37A2 genes in Xinjiang brown cattle, which are associated with mastitis resistance, were identified using a GWAS. Pyrosequencing analysis showed that the promoter methylation levels of the FHIT and PIAS1 genes in the mastitis group were higher and lower, respectively, than those in the healthy group (65.97 ± 19.82% and 58.00 ± 23.52%). However, the methylation level of the PIAS1 gene promoter region in the mastitis group was lower than that in the healthy group (11.48 ± 4.12% and 12.17 ± 4.25%). Meanwhile, the methylation levels of CpG3, CpG5, CpG8, and CpG15 in the promoter region of the FHIT and PIAS1 genes in the mastitis group were significantly higher than those in the healthy group (p < 0.01), respectively. RT-qPCR showed that the expression levels of the FHIT and PIAS1 genes were significantly higher in the healthy group than those in the mastitis group (p < 0.01). Correlation analysis showed that the promoter methylation level of the FHIT gene was negatively correlated with its expression. Hence, increased methylation in the promoter of the FHIT gene reduces the mastitis resistance in Xinjiang brown cattle. Finally, this study provides a reference for the molecular-marker-assisted selection of mastitis resistance in dairy cattle.
Genetic Control of Avian Migration: Insights from Studies in Latitudinal Passerine Migrants
Authors: Sharma Aakansha , Sur Sayantan , Tripathi Vatsala , Kumar Vinod
Published: May 2023

Twice-a-year, large-scale movement of billions of birds across latitudinal gradients is one of the most fascinating behavioral phenomena seen among animals. These seasonal voyages in autumn southwards and in spring northwards occur within a discrete time window and, as part of an overall annual itinerary, involve close interaction of the endogenous rhythm at several levels with prevailing photoperiod and temperature. The overall success of seasonal migrations thus depends on their close coupling with the other annual sub-cycles, namely those of the breeding, post-breeding recovery, molt and non-migratory periods. There are striking alterations in the daily behavior and physiology with the onset and end of the migratory period, as shown by the phase inversions in behavioral (a diurnal passerine bird becomes nocturnal and flies at night) and neural activities. Interestingly, there are also differences in the behavior, physiology and regulatory strategies between autumn and spring (vernal) migrations. Concurrent molecular changes occur in regulatory (brain) and metabolic (liver, flight muscle) tissues, as shown in the expression of genes particularly associated with 24 h timekeeping, fat accumulation and the overall metabolism. Here, we present insights into the genetic basis of migratory behavior based on studies using both candidate and global gene expression approaches in passerine migrants, with special reference to Palearctic-Indian migratory blackheaded and redheaded buntings.
The Distribution of the Genotypes of ABCB1 and CES1 Polymorphisms in Kazakhstani Patients with Atrial Fibrillation Treated with DOAC
Authors: Abdrakhmanov Ayan , Akilzhanova Ainur , Shaimerdinova Aizhan , Zhalbinova Madina , Tuyakova Gulnara , Abildinova Svetlana , Albayev Rustam , Ainabekova Bayan , Chinybayeva Assel , Suleimen Zhanasyl , Bekbossynova Makhabbat
Published: May 2023

Nowadays, direct oral anticoagulants (DOACs) are the first-line anticoagulant strategy in patients with non-valvular atrial fibrillation (NVAF). We aimed to identify the influence of polymorphisms of the genes encoding P-glycoprotein (ABCB1) and carboxylesterase 1 (CES1) on the variability of plasma concentrations of DOACs in Kazakhstani patients with NVAF. We analyzed polymorphisms rs4148738, rs1045642, rs2032582 and rs1128503 in ABCB1 and rs8192935, rs2244613 and rs71647871 CES1 genes and measured the plasma concentrations of dabigatran/apixaban and biochemical parameters in 150 Kazakhstani NVAF patients. Polymorphism rs8192935 in the CES1 gene (p = 0.04), BMI (p = 0.01) and APTT level (p = 0.01) were statistically significant independent factors of trough plasma concentration of dabigatran. In contrast, polymorphisms rs4148738, rs1045642, rs2032582 and rs1128503 in ABCB1 and rs8192935, rs2244613 and rs71647871 CES1 genes did not show significant influence on plasma concentrations of dabigatran/apixaban drugs (p > 0.05). Patients with GG genotype (138.8 ± 100.1 ng/mL) had higher peak plasma concentration of dabigatran than with AA genotype (100.9 ± 59.6 ng/mL) and AG genotype (98.7 ± 72.3 ng/mL) (Kruskal–Wallis test, p = 0.25). Thus, CES1 rs8192935 is significantly associated with plasma concentrations of dabigatran in Kazakhstani NVAF patients (p < 0.05). The level of the plasma concentration shows that biotransformation of the dabigatran processed faster in individual carriers of GG genotype rs8192935 in the CES1 gene than with AA genotype.
A Carrier Female Manifesting an Unusual X-Linked Retinoschisis Phenotype Associated with the Pathogenic Variant c.266delA, p.(Tyr89LeufsTer37) in RS1, and Skewed X-Inactivation
Authors: Kirkby Jennifer , Halford Stephanie , Shanks Morag , Moore Anthony , Gait Anthony , Jenkins Lucy , Clouston Penny , Patel Chetan K. , Downes Susan M.
Published: May 2023

X-linked retinoschisis (XLRS) is the most common juvenile macular degeneration in males. Unlike most other X-linked retinal dystrophies, carrier heterozygous females are very rarely reported to show clinical features of the disease. Herein, we describe unusual retinal features in a 2-year-old female infant with family history and genetic testing consistent with XLRS.
Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation
Authors: Kovincic Anika , Markovic Ksenija , Ristic Danijela , Babic Vojka , Petrovic Tanja , Zivanovic Tomislav , Kravic Natalija
Published: May 2023

A high level of genetic purity in crop varieties must be achieved and maintained for agronomic performance, encouraging investment and innovation in plant breeding and ensuring that the improvements in productivity and quality imparted by breeders are delivered to the consumer. Since the success of hybrid seed production is dependent upon the genetic purity of the parental lines, in this study, the experimental F1exp maize hybrid and its parental inbreeds were used as a model system to examine the discriminative power of morphological, biochemical and SSR markers for seed purity assay. The highest number of off-type plants were estimated by morphological markers. According to the comparison of prolamins and albumins banding patterns of parental and derived F1exp seeds, genetic impurities could not be detected. Molecular analysis detected two types of genetic profile irregularity. Beside its use for verifying varieties of maize, report on umc1545 primer pair ability to detect non-specific bands (i.e., off-types), in both the maternal component and F1exp, which is the first report on this issue yet, strongly supports the recommendation of this SSR marker use for more accurate and time-efficient maize hybrids and parental lines genetic pyrity testing.
Histone Deacetylases HD2A and HD2B Undergo Feedback Regulation by ABA and Modulate Drought Tolerance via Mediating ABA-Induced Transcriptional Repression
Authors: Han Yongtao , Haouel Amira , Georgii Elisabeth , Priego-Cubero Santiago , Wurm Christoph J. , Hemmler Daniel , Schmitt-Kopplin Philippe , Becker Claude , Durner Jörg , Lindermayr Christian
Published: May 2023

Histone deacetylation catalyzed by histone deacetylase plays a critical role in gene silencing and subsequently controls many important biological processes. It was reported that the expression of the plant-specific histone deacetylase subfamily HD2s is repressed by ABA in Arabidopsis. However, little is known about the molecular relationship between HD2A/HD2B and ABA during the vegetative phase. Here, we describe that the hd2ahd2b mutant shows hypersensitivity to exogenous ABA during the germination and post-germination period. Additionally, transcriptome analyses revealed that the transcription of ABA-responsive genes was reprogrammed and the global H4K5ac level is specifically up-regulated in hd2ahd2b plants. ChIP-Seq and ChIP-qPCR results further verified that both HD2A and HD2B could directly and specifically bind to certain ABA-responsive genes. As a consequence, Arabidopsis hd2ahd2b plants displayed enhanced drought resistance in comparison to WT, which is consistent with increased ROS content, reduced stomatal aperture, and up-regulated drought-resistance-related genes. Moreover, HD2A and HD2B repressed ABA biosynthesis via the deacetylation of H4K5ac at NCED9. Taken together, our results indicate that HD2A and HD2B partly function through ABA signaling and act as negative regulators during the drought resistance response via the regulation of ABA biosynthesis and response genes.
Constructing a Learning Curve to Discuss the Medical Treatments and the Effect of Vaccination of COVID-19
Authors: Chen Yi-Tui , Su Emily Chia-Yu , Hung Fang Ming , Hiramatsu Tomoru , Hung Tzu-Jen , Kuo Chao-Yang
Published: May 2023

Acknowledging the extreme risk COVID-19 poses to humans, this paper attempted to analyze and compare case fatality rates, identify the existence of learning curves for COVID-19 medical treatments, and examine the impact of vaccination on fatality rate reduction. Confirmed cases and deaths were extracted from the “Daily Situation Report” provided by the World Health Organization. The results showed that low registration and low viral test rates resulted in low fatality rates, and the learning curve was significant for all countries except China. Treatment for COVID-19 can be improved through repeated experience. Vaccinations in the U.K. and U.S.A. are highly effective in reducing fatality rates, but not in other countries. The positive impact of vaccines may be attributed to higher vaccination rates. In addition to China, this study identified the existence of learning curves for the medical treatment of COVID-19 that can explain the effect of vaccination rates on fatalities.
Accurate Prediction of Anxiety Levels in Asian Countries Using a Fuzzy Expert System
Authors: Ramzan Mouz , Hamid Muhammad , Alhussan Amel Ali , AlEisa Hussah Nasser , Abdallah Hanaa A.
Published: May 2023

Anxiety is a common mental health issue that affects a significant portion of the global population and can lead to severe physical and psychological consequences. The proposed system aims to provide an objective and reliable method for the early detection of anxiety levels by using patients’ physical symptoms as input variables. This paper introduces an expert system utilizing a fuzzy inference system (FIS) to predict anxiety levels. The system is designed to address anxiety’s complex and uncertain nature by utilizing a comprehensive set of input variables and fuzzy logic techniques. It is based on a set of rules that represent medical knowledge of anxiety disorders, making it a valuable tool for clinicians in diagnosing and treating these disorders. The system was tested on real datasets, demonstrating high accuracy in the prediction of anxiety levels. The FIS-based expert system offers a powerful approach to cope with imprecision and uncertainty and can potentially assist in addressing the lack of effective remedies for anxiety disorders. The research primarily focused on Asian countries, such as Pakistan, and the system achieved an accuracy of 87%, which is noteworthy.
Spatial Relationship between Heat Illness Incidence and Heat Vulnerability in Gurye and Sunchang, South Korea, 2018
Authors: Yi Chaeyeon , Kwon Hyukgi
Published: May 2023

Heatwaves, along with their affiliated illnesses and mortalities, are increasing in frequency and severity under climate change. Spatial analyses at the level of census output areas can produce detailed maps of heatwave risk factors and potential correlated damages, thus contributing to practical policies to reduce the risk of heatwave illnesses. This study analyzed the 2018 summer heatwave in Gurye and Sunchang counties in South Korea. To compare damages and analyze the detailed causes of heatwave vulnerability, spatial autocorrelation analyses were conducted, incorporating weather, environmental, personal, and disease factors. Gurye and Sunchang, although similar in demographics and region, exhibited large differences in heatwave damage specifically in the number of heat-related illness cases. In addition, exposure data were constructed at the census output area level by calculating the shadow pattern, sky view factor, and mean radiant temperature, revealing a higher risk in Sunchang. Spatial autocorrelation analyses revealed that the factors most highly correlated with heatwave damage were hazard factors, in the case of Gurye, and vulnerability factors, in the case of Sunchang. Accordingly, it was concluded that regional vulnerability factors were better distinguished at the finer scale of the census output area and when detailed and diversified weather factors were incorporated.
The Landscapes of Gluten Regulatory Network in Elite Wheat Cultivars Contrasting in Gluten Strength
Authors: Liu Jiajun , Li Dongsheng , Zhu Peng , Qiu Shi , Yao Kebing , Zhuang Yiqing , Chen Chen , Liu Guanqing , Wen Mingxing , Guo Rui , Yao Weicheng , Deng Yao , Shen Xueyi , Li Tao
Published: May 2023

Yangmai-13 (YM13) is a wheat cultivar with weak gluten fractions. In contrast, Zhenmai-168 (ZM168) is an elite wheat cultivar known for its strong gluten fractions and has been widely used in a number of breeding programs. However, the genetic mechanisms underlying the gluten signatures of ZM168 remain largely unclear. To address this, we combined RNA-seq and PacBio full-length sequencing technology to unveil the potential mechanisms of ZM168 grain quality. A total of 44,709 transcripts were identified in Y13N (YM13 treated with nitrogen) and 51,942 transcripts in Z168N (ZM168 treated with nitrogen), including 28,016 and 28,626 novel isoforms in Y13N and Z168N, respectively. Five hundred and eighty-four differential alternative splicing (AS) events and 491 long noncoding RNAs (lncRNAs) were discovered. Incorporating the sodium-dodecyl-sulfate (SDS) sedimentation volume (SSV) trait, both weighted gene coexpression network analysis (WGCNA) and multiscale embedded gene coexpression network analysis (MEGENA) were employed for network construction and prediction of key drivers. Fifteen new candidates have emerged in association with SSV, including 4 transcription factors (TFs) and 11 transcripts that partake in the post-translational modification pathway. The transcriptome atlas provides new perspectives on wheat grain quality and would be beneficial for developing promising strategies for breeding programs.
Investigating the Urinary Metabolome in the First Year of Life and Its Association with Later Diagnosis of Autism Spectrum Disorder or Non-Typical Neurodevelopment in the MARBLES Study
Authors: Sotelo-Orozco Jennie , Schmidt Rebecca J. , Slupsky Carolyn M. , Hertz-Picciotto Irva
Published: May 2023

Developmental disabilities are often associated with alterations in metabolism. However, it remains unknown how early these metabolic issues may arise. This study included a subset of children from the Markers of Autism Risks in Babies—Learning Early Signs (MARBLES) prospective cohort study. In this analysis, 109 urine samples collected at 3, 6, and/or 12 months of age from 70 children with a family history of ASD who went on to develop autism spectrum disorder (ASD n = 17), non-typical development (Non-TD n = 11), or typical development (TD n = 42) were investigated by nuclear magnetic resonance (NMR) spectroscopy to measure urinary metabolites. Multivariate principal component analysis and a generalized estimating equation were performed with the objective of exploring the associations between urinary metabolite levels in the first year of life and later adverse neurodevelopment. We found that children who were later diagnosed with ASD tended to have decreased urinary dimethylamine, guanidoacetate, hippurate, and serine, while children who were later diagnosed with Non-TD tended to have elevated urinary ethanolamine and hypoxanthine but lower methionine and homovanillate. Children later diagnosed with ASD or Non-TD both tended to have decreased urinary 3-aminoisobutyrate. Our results suggest subtle alterations in one-carbon metabolism, gut-microbial co-metabolism, and neurotransmitter precursors observed in the first year of life may be associated with later adverse neurodevelopment.
The Coming of Age of the P2X7 Receptor in Diagnostic Medicine
Authors: Di Virgilio Francesco , Vultaggio-Poma Valentina , Falzoni Simonetta , Giuliani Anna Lisa
Published: May 2023

The discovery of the P2X7 receptor (P2X7R, originally named P2Z) in immune cells, its cloning, and the identification of its role in a multiplicity of immune-mediated diseases raised great hopes for the development of novel and more potent anti-inflammatory medicaments. Unfortunately, such hopes were partially deluded by the unsatisfactory results of most early clinical trials. This failure substantially reduced the interest of the pharmaceutical and biotech industries in the clinical development of P2X7R-targeted therapies. However, recent findings ushered in a second life for the P2X7R in diagnostic medicine. New P2X7R radioligands proved to be very reliable tools for the diagnosis of neuroinflammation in preclinical and clinical studies, and detection and measurement of free P2X7 receptor (or P2X7 subunit) in human blood suggested its potential use as a circulating marker of inflammation. Here we provide a brief review of these novel developments.
The Aging Enteric Nervous System
Authors: Nguyen Tinh Thi , Baumann Peter , Tüscher Oliver , Schick Sandra , Endres Kristina
Published: May 2023

The gut and the brain communicate via the nervous system, hormones, microbiota-mediated substances, and the immune system. These intricate interactions have led to the term “gut-brain axis”. Unlike the brain—which is somewhat protected—the gut is exposed to a variety of factors throughout life and, consequently, might be either more vulnerable or better adapted to respond to these challenges. Alterations in gut function are common in the elder population and associated with many human pathologies, including neurodegenerative diseases. Different studies suggest that changes in the nervous system of the gut, the enteric nervous system (ENS), during aging may result in gastrointestinal dysfunction and initiate human pathologies of the brain via its interconnection with the gut. This review aims at summarizing the contribution of normal cellular aging to the age-associated physiological changes of the ENS. Morphological alterations and degeneration of the aging ENS are observed in different animal models and humans, albeit with considerable variability. The aging phenotypes and pathophysiological mechanisms of the aging ENS have highlighted the involvement of enteric neurons in age-related diseases of the central nervous system such as Alzheimer’s or Parkinson’s disease. To further elucidate such mechanisms, the ENS constitutes a promising source of material for diagnosis and therapeutic predictions, as it is more accessible than the brain.
Artificial Intelligence and Machine Learning in the Diagnosis and Management of Stroke: A Narrative Review of United States Food and Drug Administration-Approved Technologies
Authors: Chandrabhatla Anirudha S. , Kuo Elyse A. , Sokolowski Jennifer D. , Kellogg Ryan T. , Park Min , Mastorakos Panagiotis
Published: May 2023

Stroke is an emergency in which delays in treatment can lead to significant loss of neurological function and be fatal. Technologies that increase the speed and accuracy of stroke diagnosis or assist in post-stroke rehabilitation can improve patient outcomes. No resource exists that comprehensively assesses artificial intelligence/machine learning (AI/ML)-enabled technologies indicated for the management of ischemic and hemorrhagic stroke. We queried a United States Food and Drug Administration (FDA) database, along with PubMed and private company websites, to identify the recent literature assessing the clinical performance of FDA-approved AI/ML-enabled technologies. The FDA has approved 22 AI/ML-enabled technologies that triage brain imaging for more immediate diagnosis or promote post-stroke neurological/functional recovery. Technologies that assist with diagnosis predominantly use convolutional neural networks to identify abnormal brain images (e.g., CT perfusion). These technologies perform comparably to neuroradiologists, improve clinical workflows (e.g., time from scan acquisition to reading), and improve patient outcomes (e.g., days spent in the neurological ICU). Two devices are indicated for post-stroke rehabilitation by leveraging neuromodulation techniques. Multiple FDA-approved technologies exist that can help clinicians better diagnose and manage stroke. This review summarizes the most up-to-date literature regarding the functionality, performance, and utility of these technologies so clinicians can make informed decisions when using them in practice.
The Emerging Role of Icosapent Ethyl in Patients with Cardiovascular Disease: Mechanistic Insights and Future Applications
Authors: Gupta Ashish , Alkhalil Mohammad
Published: May 2023

Omega-3 polyunsaturated fatty acids (PUFAs) were early established as therapeutic option for patients with high triglyceride levels. Their effects on lipoprotein particles, including a reduction in very low-density lipoprotein and a shift from small to large low-density lipoprotein, is increasingly recognised. This is coupled with their ability to be incorporated within the cellular membrane, leading to plaque stability and anti-inflammatory effects. Nonetheless, recent clinical trials have not been consistent in demonstrating the potential cardioprotective effects of omega-3 fatty acids. This is despite the circumstantial evidence from imaging studies illustrating the stabilising effects on atherosclerotic plaques and slowing of plaque progression. In this article, we will review the effects of omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on lipid biomarkers, atherosclerotic plaque features, and clinical outcome studies and provide a mechanistic role in managing residual risk of atherosclerosis. This will provide better insight into the inconsistency of the recently reported clinical outcome studies.
Leakage Flow Characteristics in Blade Tip of Shaft Tubular Pump
Authors: Lin Zhikang , Yang Fan , Guo Jun , Jian Hongfu , Sun Shengjie , Jin Xiaoyu
Published: May 2023

The shaft tubular pump device is widely used in various water diversion projects because of its ultra-low head and large flow characteristics. Due to the tip clearance between the blade and the shroud, it is easy to cause hydraulic mechanical performance changes, induced vibration, and noise, which seriously affects the safe and stable operation of the pump. Steady and unsteady three-dimensional flow field numerical simulations of a shaft tubular pump device were carried out using computational fluid dynamics to investigate the impeller flow properties of the device under various flow conditions, including the tip clearance leakage flow (TCLF) and change rule of pressure pulsation. The TCLF, vortex morphology evolution, and pressure pulsation properties of the impeller tip clearance were analyzed. The results show that with an increase in the flow rate, the influence of the tip clearance size on the tip clearance flow decreases, the TCLF decreases, and the axial velocity of the water flow at the tip clearance increases. When the flow rate increases, the swirling strength of the tip leakage vortex decreases, and the distance between the tip leakage vortex and the suction surface of the blade increases. With the increase in flow rate, the pressure pulsation amplitude at the tip clearance increases first and then decreases. The focus of this study is to analyze the variation of tip clearance flow field and pressure pulsation under multiple working conditions, aiming to provide some help for improving the performance of the pump device and ensuring its safe operation.
Evidence of a Natural Hybrid Oomycete Isolated from Ornamental Nursery Stock
Authors: Benavent-Celma Clara , McLaggan Debbie , van West Pieter , Woodward Steve
Published: May 2023

The oomycete genus Phytophthora includes many plant pathogens important in agricultural and environmental systems. Natural interspecific hybridization has been reported several times in Phytophthora, and although the fundamental processes of interspecific hybridization and the consequences of subsequent ecological distribution are poorly understood, reports suggest some hybrids can infect a broader host range and display enhanced virulence compared to the putative parental species. During a survey carried out at the University of Aberdeen in 2014–2015, of oomycetes present in ornamental plants purchased via the internet, a batch of oomycete isolates remained unidentified, showing, in some isolates, features generally related to hybridization. The aim of this study was to determine whether hybridization events had occurred between endemic and introduced oomycetes, probably/possibly facilitated through the international plant trade. The list of isolates examined included a putative hybrid closely related to Phytophthora cryptogea. The putative hybrid isolate was further characterized, and pathogenicity were tests carried out on Eucalyptus globulus, using an isolate of P. cryptogea as a positive control. Cloning of ITS, COXI and β-tubulin genes resulted in different sequence versions of the putative hybrid isolate; after mapping and a polymorphism position comparison, it was concluded that the studied isolate contained genetic information from P. cryptogea, P. erythroseptica, P. kelmanii, P. sansomeana and Phytopythium chamaehyphon. A PCR-RFLP assay, a NEBcutter analysis and flow cytometry analysis (genomes ranged between 0.168 to 0.269 pg/2C) added further evidence of the hybrid nature of this isolate. The putative hybrid presented complex growing patterns ranging from rosaceous to chrysanthemum-like and had an optimum growth temperature of 25 °C. Although the putative hybrid produced visible symptoms of disease on E. globulus seedlings, assessment of the relative susceptibility of E. globulus to P. cryptogea and the putative hybrid indicated that P. cryptogea was significantly more virulent than the putative hybrid, based on mortality, disease severity and foliar symptoms.
Transcriptional Alterations Induced by Delta-9 Tetrahydrocannabinol in the Brain and Gonads of Adult Medaka
Authors: Vassall Marlee , Chakraborty Sourav , Feng Yashi , Faheem Mehwish , Wang Xuegeng , Bhandari Ramji Kumar
Published: May 2023

With the legalization of marijuana smoking in several states of the United States and many other countries for medicinal and recreational use, the possibility of its release into the environment cannot be overruled. Currently, the environmental levels of marijuana metabolites are not monitored on a regular basis, and their stability in the environment is not well understood. Laboratory studies have linked delta 9-tetrahydrocannabinol (Δ9-THC) exposure with behavioral abnormalities in some fish species; however, their effects on endocrine organs are less understood. To understand the effects of THC on the brain and gonads, we exposed adult medaka (Oryzias latipes, Hd-rR strain, both male and female) to 50 ug/L THC for 21 days spanning their complete spermatogenic and oogenic cycles. We examined transcriptional responses of the brain and gonads (testis and ovary) to Δ9-THC, particularly molecular pathways associated with behavioral and reproductive functions. The Δ9-THC effects were more profound in males than females. The Δ9-THC-induced differential expression pattern of genes in the brain of the male fish suggested pathways to neurodegenerative diseases and pathways to reproductive impairment in the testis. The present results provide insights into endocrine disruption in aquatic organisms due to environmental cannabinoid compounds.
UPO Biobank: The Challenge of Integrating Biobanking into the Academic Environment to Support Translational Research
Authors: Bettio Valentina , Mazzucco Eleonora , Aleni Chiara , Cracas Silvia , Rinaldi Carmela , Antona Annamaria , Varalda Marco , Venetucci Jacopo , Ferrante Daniela , Rimedio Antonio , Capello Daniela
Published: May 2023

Biobanks are driving motors of precision and personalized medicine by providing high-quality biological material/data through the standardization and harmonization of their collection, preservation, and distribution. UPO Biobank was established in 2020 as an institutional, disease, and population biobank within the University of Piemonte Orientale (UPO) for the promotion and support of high-quality, multidisciplinary studies. UPO Biobank collaborates with UPO researchers, sustaining academic translational research, and supports the Novara Cohort Study, a longitudinal cohort study involving the population in the Novara area that will collect data and biological specimens that will be available for epidemiological, public health, and biological studies on aging. UPO Biobank has been developed by implementing the quality standards for the field and the ethical and legal issues and normative about privacy protection, data collection, and sharing. As a member of the “Biobanking and Biomolecular Resources Research Infrastructure” (BBMRI) network, UPO Biobank aims to expand its activity worldwide and launch cooperation with new national and international partners and researchers. The objective of this manuscript is to report an institutional and operational experience through the description of the technical and procedural solutions and ethical and scientific implications associated with the establishment of this university research biobank.
Long-Term Dynamics of Viticultural Landscape in Cyprus—Four Centuries of Expansion, Contraction and Spatial Displacement
Authors: Papadias Evangelos , Detsis Vassilis , Hadjikyriacou Antonis , Papadopoulos Apostolos G. , Vradis Christoforos , Chalkias Christos
Published: May 2023

Viticulture has historically been an important part of the social and economic life in the Mediterranean, while wine is reckoned among the oldest documented trades. The aim of the study is to record, evaluate and analyze spatial data from historical sources in order to gain insights into the dynamics of the viticultural landscape from the beginning of the Ottoman period to the present day. The study was based on (a) three historical maps published in 1885, 1942 and 1969, (b) records from historical surveys—two from the Ottoman period (1572 fiscal survey, 1832/33 property survey) and the British agricultural census of 1931, (c) present-day records from the vineyard survey of 2009 carried out by the Republic of Cyprus. In the beginning of the study period the center of viticulture was well established within the area of the southern and eastern slopes of Troodos massif. The vineyards expanded mainly around the same growing area until WW2 when they gradually began to be relocated in southwest direction to lower altitudes. This long-term trajectory of spatial patterns was driven by external demand for the product but also by the interplay of environmental, topographic and cultural factors, as well as by the state’s policy framework which largely reflected long-term Mediterranean-wide patterns.
The Spatial Pattern and Influencing Factors of Traffic Dominance in Xi’an Metropolitan Area
Authors: Li Julin , Li Tongsheng , Zhu Bingchen , Wang Yilin , Chen Xieyang , Liu Ruikuan
Published: May 2023

Metropolitan areas shoulder the crucial task of regional and even national economic development. Analyzing the spatial patterns and influencing factors of metropolitan traffic dominance can provide a scientific basis for the optimization of its transportation and industrial layout, which is conducive to the development of its economy. Previous studies on traffic dominance paid little attention to metropolitan areas and even less so to study these areas from the perspective of town units, even though these are the basic units that narrow the gap between urban and rural areas and thus achieve regional economic integration. The traffic dominance model can comprehensively and wholly reflect regional traffic conditions, due to its multidimensional characteristics (including traffic network density, traffic arterial influence and accessibility). Consequently, taking the Xi’an metropolitan area as an exemplar and the town as the basic unit, this paper employs this model and other methods to study the spatial pattern and influencing factors of its traffic dominance. The results show the following: (1) the traffic network density, traffic arterial influence and accessibility had different distribution patterns; however, they were the same in that their superiorities were relatively high in the main urban area of Xi’an city or along and on both sides of the high-speed railways (HSRs), whereas relatively low in the peripheral areas; (2) the integrated traffic dominance consistently displayed a “point-axis” pattern, with greater superiority in the east–west axis areas within 30 km of Xi’an city, especially in the main urban area of Xi’an city; (3) the integrated traffic dominance between towns had stable agglomeration correlation in the global areas and formed three major modes in the local areas: high–high, low–low and low–high aggregation. High–high mode was concentrated in the main urban areas of Xi’an and Xianyang city, low–low mode was mainly distributed in the Weibei hilly and gully areas and the Qinling mountain areas and low–high mode always nested on the edge of high–high areas; and (4) the location, GDP and elevation had a greater impact, whereas construction intensity, population and slope had a relatively small influence on the town’s traffic dominance, and their influence ability had a decreasing trend from 2010 to 2022. Finally, this paper discusses the theoretical implications, practical values and some prospects based on the research results.
Reflecting on the Concept of Local Agroecological Food Systems
Authors: Sanz-Cañada Javier , Sánchez-Hernández José Luis , López-García Daniel
Published: May 2023

Despite the extensive literature on Local Agro-food Systems (LAFS), which involves research on local food identity and organisational proximity, the environmental sustainability of these systems has rarely been addressed. This paper develops a new concept called Local Agroecological Food Systems (LAEFS), which focuses the research not only on local food identity, but also on agroecological principles. We aim to conduct a reflexive review of the literature on the conceptual factors attempting to describe the particular characteristics of LAEFS (distinguishing these from LAFS). We explore five axes of analysis: (a) to establish a compromise at the local level between agro-food sectoral specialisation on the one hand and greater cultivated biodiversity and a more diversified economic structure on the other; (b) to geographically and commercially shorten food channels to the fullest extent; (c) to construct new institutional formulae in the fields of logistics, distribution and public procurement for the scaling up of sustainable food; (d) to develop a participatory, bottom-up, multi-stakeholder and multi-level territorial governance; and (e) to reduce the metabolic profile of food systems by reorganising rural-urban linkages. One of the principal objectives of LAEFS should involve redesigning agricultural and food systems at a scale greater than that of the farm (territory or landscape). This requires both a major public policy push and sustainable territorial governance that incorporate an approach based on territory, food systems and agroecology.