Selection of oligonucleotide primers for identification of genetic material of bovine leukemia virus by polymerase chain reaction
Authors:   Saratov State University of Genetics, Biotechnology and Engineering named after N. I. Vavilov, Saratov, Russia, Agaltsov Valery A. , Padilo Larisa P. ,   Saratov State University of Genetics, Biotechnology and Engineering named after N. I. Vavilov, Saratov, Russia, Pochepnya Ekaterina S. ,   Saratov State University of Genetics, Biotechnology and Engineering named after N. I. Vavilov, Saratov, Russia, Biryukova Oksana P. ,   Saratov State University of Genetics, Biotechnology and Engineering named after N. I. Vavilov, Saratov, Russia, Popova Olga M. ,   Saratov State University of Genetics, Biotechnology and Engineering named after N. I. Vavilov, Saratov, Russia, Sibgatullova Adylya K. ,   Ulyanovsk State Agrarian University named after P. A. Stolypin, Ulyanovsk, Russia, Chernykh Oleg Yu. ,   Kuban State Agrarian University named after I. T. Trubilin, Krasnodar, Russia; North Caucasian Zonal Scientific Research Veterinary Institute (SKZNIVI) – branch of Federal Rostov Agrarian Scientific Center, Novocherkassk, Russia, Shevchenko Alexander N. ,   Kuban State Agrarian University named after I. T. Trubilin, Krasnodar, Russia, Belousov Vasiliy I. ,   Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K. I. Skryabin”, Moscow, Russia, Koba Igor S. ,   Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K. I. Skryabin”, Moscow, Russia
Published: January 2025

The article presents the results of primer selection for the analysis of enzootic bovine leukemia virus by real-time polymerase chain reaction (PCR-RT). Currently, there is no treatment for bovine leukemia, so it is necessary to identify this virus using diagnostic test systems. Today, it is necessary to carry out measures to prevent and control bovine leukemia. Preventing the import of animals from farms unfavorable for bovine leukemia; 30-day quarantine of newly imported animals with serological testing of their blood serum for leukemia; strict adherence to the rules of asepsis and antisepsis during veterinary and zootechnical treatment of animals and the timing of scheduled diagnostic tests, as well as regular preventive disinfection, etc. Animal leukemia can only be detected by laboratory methods and eliminated through ongoing, systematic work. The fight against this disease today can be carried out only by slaughtering sick livestock, raising animals free from the bovine leukemia virus.
For diagnostics of BLV there are a number of methods based on serological (RID and ELISA) and molecular genetic (PCR) studies. The selection of primers was carried out by the ENV gene. A fragment of the glycoprotein gene (ENV) of the bovine leukemia virus, involved in encoding two proteins gp85 and gp37, was chosen as a target.
The presented oligonucleotides will allow identifying isolates and strains of RNA of the bovine leukemia virus. The primers we selected have high specificity, sensitivity of identification of the enzootic bovine leukemia virus. The result of the invention is a reduction in the time for conducting mass studies of samples for the presence of the genome of the enzootic bovine leukemia virus. The use of the kit will help reduce the time when conducting mass studies of samples for the presence of the genome of the enzootic bovine leukemia virus. The presented results of the reactions indicate their good reproducibility and reliability.