bluuebunny/crossref_metadata_2025 huggingface dataset:
We Won’t be Able to Change How We Fight Without Changing How We Love
Authors: Ratele Kopano
Published: March 2024

This article draws from and elaborates on some ideas conveyed in my book, Why Men Hurt Women and Other Reflections on Love, Violence and Masculinity (2022), to reflect on the relationship between love and violence. It contends that as a society we will not be able to change how we fight or oppose each other, at least we will find it more difficult to reduce the magnitude of men’s violence against women, without changing the dominant narrative of love. Although the reflection on the relation between violence and love applies to other kinds of aggression and intimacy, such as between men’s violence against and love for other men, the focus of this article is men’s violence against women they love. Love and violence, the article shows, are not necessarily and always incompatible but instead are sometimes nested in each other.
Dressing up the Self: Feminism and the Anomalous Art of Zanele Muholi and Cindy Sherman
Authors: Viljoen Stella
Published: March 2024

For Lauren Berlant (1998), intimacy begins with shared narratives or narratives about something shared. In other words, we desire our story as humans to be set within ‘zones of familiarity and comfort’ (Berlant, 1998: 281). How do we know we have achieved familiarity and comfort? Berlant says, that we know it is enough to intimate or gesture, to communicate with brevity because of a communal language (like the intimacy of a shared joke). But, says Berlant (1998: 281), ‘the inwardness of the intimate is met by a corresponding publicness’. This ‘public’ side is related to what she terms the ‘institutions of intimacy’ that we create in the hope that these will give us ‘a life’ (by which she presumably means a life of intimacy). Might Berlant consider Art as an institution of intimacy, a means of creating a shared language by which we can enter into zones of familiarity and comfort but also by which we can point out the flaws in each other’s thinking and laugh together at the ways in which we have failed at intimacy? Berlant describes a tension between desire and ‘therapy’ (or what one might think of as a response to immorality) and says this tension governs our ‘modern, mass-mediated sense of intimacy’. The article explores whether one might think of feminism in the photographic self-portraits of Cindy Sherman and Zanele Muholi as a form of ‘therapy’, a means of correcting the violence we commit both knowingly and, as is often the case, out of a kind of willing ignorance.
An Illuminated Darkness
Authors: Coetzee Jacques
Published: March 2024

An Illuminated Darkness
Ketersediaan N dan Fe-larut pada Tanah Ultisol yang diaplikasikan Lumpur Kolam Ikan
Authors: Meiliyansari Syafira Rossa , Priatmadi Bambang Joko , Sari Nukhak Nufita
Published: March 2024

The use of fish pond mud in improving soil quality is generally still not widely used. This study used various doses of pond mud to determine the effect of pond mud doses on the availability of N and Fe-soluble in Ultisol soil planted with mustard greens. The research was conducted in the Greenhouse in April - May 2022 with a Completely Randomized Design (CRD), five doses of fish pond sludge namely at doses of 0; 0.5: 7.5: 10; and 12.5% applied to Ultisol soil. The results showed that applying fish pond sludge significantly affected soil pH, N-NO3-, N-NH4+ and Fe-soluble. Soil pH decreases due to a reaction between water and Fe compounds that bind OH- and release H+. Whereas N-NO3-, N-NH4+, and soluble-Fe significantly increased due to chemical reactions that produced soluble salts which reacted with water. As for the soil parameters, the effect of pond mud decreases soil pH at all doses of addition of mud and increases N-NO3-, N-NH4+, and soluble-Fe Ultisol, with the best doses of M3 (equivalent to 1 kg mud + 9 kg of soil). There is a relationship between the chemical properties of the soil and the fresh weight of the plants, as evidenced by the increase in the wet weight of the mustard plants.
Efektivitas Pupuk Organik Cair Bawang Merah dan Limbah Bawang Merah Terhadap Perubahan Sifat Kimia Tanah dan Pertumbuhan Bawang Merah
Authors: Nathan Muhammad , Jayadi Muh. , Thamrin Hidayana
Published: March 2024

Soil has an important role "in agriculture for the growth and production" of plants. The combination of organic and inorganic fertilizers can increase crop productivity and improve soil quality on land so that it can be used sustainably. One of the potential plants that can be used as a basic ingredient of organic fertilizer is red onion. This study aims to determine the effect of red onion liquid organic fertilizer on changes in soil chemical properties and plant growth. This research was conducted using a factorial randomized block design (RBD) with 2 factors. The first factor is the dose of organic fertilizer and the second factor is the dose of inorganic fertilizer. Parameters observed were pH, C-Organic, N, P, K, tuber weight, plant fresh weight, number of leaves and plant height. The results showed that red onion organic fertilizer can lower soil pH and increase soil C-Organic, N, P and K. The combination of the D1KB2 treatment (50% basic fertilizer + 15,000 ppm red onion waste) gave the best results in increasing soil organic C, Phosphate and Potassium. Conclusion. The interaction of inorganic fertilizers and red onion liquid organic fertilizer had a significant effect on tuber weight and plant wet weight, and had no significant effect on plant height and number of leaves.
Faktor yang Mempengaruhi Keputusan Pembelian Sayuran di Agrowisata Barro Tani Manunggal Desa Kepatihan Kecamatan Selogiri Kabupaten Wonogiri
Authors: Listyawati Desi , Dewati Rosita , Arianti Yoesti Silvana , Setyarini Agung
Published: March 2024

Vegetables are one of the most important food sources in supporting a healthy body. The alternative to buy vegetabes is in agrotourism. Barro Tani Manunggal Agrotourism is one of tourism places that provides or sells vegetable commodities. In maintaining agrotourism, it is necessary to know consumer preferences for vegetable products. This study aimed to determine the characteristics of vegetable consumers and the influence factors of consumer preferences for vegetable purchasing decisions at Barro Tani Manunggal Agrotourism. The sampling technique used purposive sampling method. The number of respondents was 56 respondents with the criteria of more than one time bought vegetables in Barro Tani Manunggal Agrotourism. The data analysis methods are t-test, F-test, and coefficient determination. The results show that the average respondent who shopped for vegetable is in aged 15-64 years, most of whom have high schoo or equivalent education. Most of the respondents are entrepreneurs, and the average income of respondents in this study is IDR 2.000.001-3.000.000. The variables of service speed and product availability have a positive and significant effect on purchasing decisions for vegetables in Barro Tani Manunggal Agrotourism. The freshness variable has a negative and significant effect. While the variables of pricing, product quality, location, and convenience do not have a significant effect on vegetable purchasing decisions.
DEVELOPMENT OF WEB GIS APPLICATIONS FOR MONITORING AGRICULTURAL ACTIVITIES IN SUNGAI KAKAP DISTRICT, KUBU RAYA REGENCY
Authors: Sugiarto Agus , Tampubolon Budiman , Christanto Ludovicus Manditya Hari
Published: March 2024

Good agricultural development planning requires an accurate and real-time database. This is very important to see the development and level of welfare of farmers as the spearhead of food security. Sungai Kakap Sub-district, Kubu Raya Regency is one of the sub-districts with the number of Gapoktan (23), Poktan (284), the number of members (6305) with Field Agricultural Extension Workers (PPL) since 2019 totalling 9 civil servants and 14 people helping themselves. The number of PPLs is not proportional to the number of farmer group members, making supervision of agricultural activities difficult. The presentation of information in the form of Web GIS is expected to be a solution so that assistance, guidance, and counselling carried out by the government through the agriculture office can be right on target. The purpose of this research is to build a Web GIS-based Agricultural Information System in Sungai Kakap District so that it can be used as a model for agricultural monitoring activities in other areas. The engineering method in this research uses Remote Sensing and Waterfall methods. Remote Sensing is a method of building spatial data using aerial photo interpretation techniques, while Waterfall is used to build a web GIS as a place to display spatial data. The result of this research is Software Engineering in the form of a Web GIS-based Agricultural Information System Application. This Web GIS application can then be used as a tool to monitor the agricultural activities of each farmer group member in each farmer group.
MAPPING OF LANDSLIDE-PRONE AREAS ON ENDE-WOLOWARU ROAD SECTION ENDE REGENCY BASED ON GEOGRAPHIC INFORMATION SYSTEM
Authors: Kae Maria Sonya , Rahmawati Arfita , Pamungkas Bella Theo Tomi
Published: March 2024

Landslides on the Ende - Wolowaru road, Ende Regency often occur every year, especially in the rainy season. Therefore, research was carried out to determine the level of vulnerability to landslides on the Ende - Wolowaru road section, Ende Regency, using an estimation model referring to Puslittanak research in 2004. Mapping of landslide-prone areas was carried out at a radius of 250 meters along the Ende - Wolowaru road section, Ende Regency with area 2,514 ha. The analysis used GIS analysis, such as: scoring and overlay methods based on 2004 Puslittanak Department of Agriculture estimation model. The parameters used are rainfall, rock type, slope, land cover and soil type. The results of the analysis in this study show that the level of vulnerability to landslides at the research location consists of a non-prone class covering an area of 13.38 ha, a vulnerable class covering an area of 1,620.38 ha and a very vulnerable class covering an area of 880.65 ha and (2) the level of validation mapping results that is equal to 88%.
DEVELOPMENT OF SUSTAINABLE TOURISM INSTRUMENTS FOR ECOLOGICAL RESILIENCE IN PARI ISLAND, DKI JAKARTA
Authors: Samadi Samadi , Munandar Aris
Published: March 2024

The research objective is to develop an instrument for assessing sustainable tourism in the context of ecological resilience in Pari Island, DKI Jakarta. This study uses research and development methods through the ADDIE model approach. The research sample includes potential and factual coral reef areas for tourism in Pari Island. The results of the study concluded that the validation of important instruments includes: (1) natural/biological criteria, namely unique and beautiful coral reefs, quite unique tidal phenomena, endemic flora and fauna such as seagrass and fish habitat, as well as other aquatic biota, ease of observing aquatic biota, opportunities for trekking, snorkeling, etc. as well as the support of comfortable temperature and humidity, normal rainfall conditions, as well as well-managed household industrial waste; and (2) Physical environmental criteria such as dominant beaches, controlled coastal land conversion rate and protected from pollution, ease of utilizing rocky beaches for friendly productive activities. The environment, such as maintaining fish, marine tourism, and others as well as the existence of a map of the distribution of coral reefs that accurately depicts local potential as well as information related to the low risk of natural disasters (landslides, floods, tsunamis). 
SPATIAL DISTRIBUTION OF TOPONYMY IN KARST AREAS GIRITONTRO
Authors: Wijayanti Pipit , Widiyanti Baiq Liana , Noviani Rita , Nursaly Baiq Rismarini
Published: March 2024

Every object in the form of an earth element or man-made feature has a name with various origins. The origins of place names can be categorized based on geography, history, folklore/culture and language so that their spatial distribution can be known. This research explores the spatial distribution of the origins of naming earth elements and man-made features in the Giritontro District. The study begins by identifying the aspects of the earth's appearance and man-made features and then finding the meaning of naming each identified element. Data collection was carried out using in-depth interviews. Determining the sources used the snowball sampling method. The results of the research show that the toponymically identified objects are dominated by man-made features with a spatial distribution of 92% in Giritontro Village, 72% in Pucanganom Village, 52% in Tlogosari Village and 78% in Jatirejo Village. The remainder is dominated by the earth's elements of the Karst Area, such as caves, hills, and springs, with a spatial distribution of Bayemharjo Village at 63%, Ngargojarjo at 53% and Tlogoharjo Village at 66%. Meanwhile, the spatial distribution of toponymy in Giritontro District, whose origin is dominated by language, is known to be spread across Giritontro District with a percentage of 60%. Apart from that, the toponymy found was predominantly geographically dispersed across Pucanganom Village with a portion of 40%, Tlogosari Village at 60%, Jatirejo Village at 61%, Bayemharjo Village at 61%, Ngargoharjo Village at 57% and Tlogoharjo Village 46%. The geographical conditions referred to are those attached to the object or the object's proximity to geographical things (associations) which are dominant in the Karst Area. 
FLOOD HAZARD MAPPING BASED ON MULTI-CRITERIA SPATIAL ANALYSIS IN THE SAMIN WATERSHED, INDONESIA
Authors: Sholeh Sofyan , Muryani Chatarina , Suryanto Suryanto
Published: March 2024

The Samin watershed which is located in the Mount Lawu area is vulnerable to flood disasters due to human activities. This research was carried out by inventorying parameter data to create a flood disaster vulnerability map using a GIS-based multi-criteria spatial approach. The seven parameters used in flood disaster analysis are Elevation, Slope, Distance from River, Drainage density, Topographic Wetness Index (TWI), Landuse, Rainfall, Type of Soil, Geology. The weight of each parameter is determined using the Analytical Hierarchy Process (AHP) which has the driving factors for flood disasters. The flood hazard map was obtained using a weighted overlay method and grouped into five classes, namely very low, low, medium, high and very high. The results of the analysis show that 11.36% of the study area has a very low hazard, 27.10% has a low hazard, 39.57% has a medium hazard, 20.43% has a high hazard and 1.54% has a very high hazard.
Kelayakan Masker Tradisional Biji Pepaya Dan Ketan Hitam Untuk Perawatan Kulit Wajah Kering
Authors: Azzahra Sairi Fathya , Guzeynova Monica Zabrina , Laila Putri Tiara , Rosalina Linda , Saputra Indra
Published: March 2024

Skin problems, especially on the face, are things that are considered to reduce appearance and have to spend a lot of money even though treating the face with natural ingredients is safer because it does not contain a mixture of chemicals. The natural ingredients in question are: Papaya seeds contain papain enzymes that can prevent wrinkles, shrink pores and moisturize the skin. Black sticky rice in addition to being a food ingredient can also be beneficial for skin beauty which can slow down premature aging, because it contains high antioxidants and anthocyanins, vitamin E and high fiber. This study aims to find out how the physical properties of papaya and black glutinous rice seed masks include color, aroma, texture, and preferences of panelists. This research is an experimental research with the object of research on papaya seed masks and black sticky rice. This type of research is quantitative using experimental methods.The data collection method used observation and testing of panelists' preferences for color, aroma, texture, adhesion of 2 makeup and beauty lecturers, 10 female students within the scope of UNP. The results showed that there was an influence of the use of papaya seed masks and black glutinous rice on dry skin care and had gray color criteria from mixing papaya seed powder and black glutinous rice, fragrant and delicious aroma, very smooth texture, moist, good elasticity felt tight and chewy.
Pengembangan Multimedia Video Tutorial Penataan Rambut Gala style dengan Teknik Kepang
Authors: Aisyah Fourindha  , Pipin Tresna Prihatin  , Asri Wibawa Sakti
Published: March 2024

Penataan rambut merupakan seluruh tahapan mulai dari pencucian, pratata hingga tindakan memperindah bentuk rambut. Tujuan penelitian ini mengidentifikasi penggunaan media pada materi penataan rambut di mata kuliah Tata Kecantikan Rambut, merancang dan membuat multimedia video tutorial penataan rambut gala style dengan teknik kepang, melakukan validasi kepada ahli materi dan ahli multimedia dan uji coba pengguna serta melakukan analisis dan revisi untuk mengetahui kelayakan multimedia. Metode yang digunakan pada penelitian ini berupa metode Research and Development (R&D) yang digunakan untuk menghasilkan produk dan menguji keefektifan produk tersebut. Prosedur pengembangan multimedia video tutorial melalui : (1) Tahap Identifikasi, (2) Tahap Perencanaan, (3) Tahap Pembuatan, (4) Tahap Validasi, (5) Tahap Revisi, (6) Tahap uji coba pengguna. Hasil validasi multimedia video tutorial oleh ahli materi diperoleh nilai persentase rata-rata sebesar 92,1%. Hasil validasi ahli multimedia diperoleh nilai persentase rata-rata sebesar 92%. Hasil uji coba pengguna diperoleh nilai persentase rata-rata sebesar 88,6%. Temuan dalam penelitian ini menunjukkan bahwa multimedia video tutorial penataan rambut gala style dengan teknik kepang yang dibuat berada pada kategori sangat baik dan sangat layak untuk digunakan sebagai multimedia pembelajaran karena hanya memerlukan sedikit perbaikan pada multimedia video tutorial yang telah dibuat dan materi yang telah disajikan.
Adaptasi Tokoh Makima Dalam Anime Chainsaw Man Pada Make Up Karakter
Authors: Arisanty Regita , Mila Karmila
Published: March 2024

Anime is a typical Japanese animation which is usually characterized by colorful pictures featuring characters in various locations and stories, aimed at various types of audience. One of the famous animation in Japan is the animation Chainsaw Man. Makima is one of the most beautiful and strongest characters from the animation Chainsaw Man whose appearance is normal, has long pink hair tied back and also has a characteristic orange eyeball. Behind her beautiful and calm face, Makima is known as a very cunning and manipulative figure in the animation Chainsaw Man. Character make-up is a make-up that is applied to change a person's appearance in terms of age, character, face, ethnicity, and nation so that it matches the character being played. The purpose of this research is to apply the basis of make-up in making character make-up by adapting the character Makima, developing the knowledge and skills acquired during college, especially in the concentration of make-up.
Pengembangan Prototipe Alat Double Mini Trampoline (DMT) Pada Cabang Olahraga Senam Trampoline DKI Jakarta
Authors: Chandra  , Yansen H. Jutalo
Published: March 2024

Tujuan penelitian ini adalah Membuat produk model pengembangan alat matras lantai menjadi flexi mat roll dapat digunakan dalam latihan bagi atlet senam artistik dan Membuat produk model pengembangan alat lantai mejadi flexi mat roll dapat digunakan untuk perlombaan bagi atlet senam artistik. Metode penelitian yang digunakan adalah metode penelitian dan pengembangan (Research and Development/ R&D). Impelemtasi model adalah menyampaikan hasil pengembangan prioduk alat matra flexi mat roll (dari proses, prosedur dan produk) kepada para pengguna professional melalui forum pertemuan atau menuliskan dalam jurnal, atau dalam buka atau handbook. Untuk mengetahui keberhasilan implementasi model (final produk), maka dilakukan evaluasi untuk menguji kelayakan serta ketercapaian tujuan produk akhir dalam penelitian yang telah dikembangkan oleh peneliti.
Analisis Bibliometrik Kajian Sport Recovery Pada Skripsi Mahasiswa Program Studi Ilmu Keolahragaan Universitas Negeri Jakarta Tahun 2011-2022
Authors: Prasanty Kusuma Wardani  , Setiakarnawijaya Yasep , Robianto Agung , Junaidi Junaidi , Tangkudung Albert Wolter Aridan , Wasan Aan
Published: March 2024

Abstrak. Penelitian analisis bibliometrik ini bertujuan untuk memetakan, menganalisis perkembangan, menganalisis terminologi yang relevan dan menemukan ide future research pada kajian sport recovery. Penelitian ini menggunakan teknik total sampling dengan jumlah sampel sebanyak 517 judul skripsi program studi Ilmu Keolahragaan dengan tahun terbit 2011-2022 dengan data yang di ambil dari laman repository.unj.ac.id. Sampel dikumpulkan dengan format Comma Separated Value (CSV), dan penelitian metode analisis bibliometrik ini menggunakan aplikasi VOSviewer. Hasil pemetaan menunjukkan bahwa kata kunci sport recovery yang berada pada cluster 7 merupakan salah satu topik penelitian yang penting pada skripsi program studi Ilmu Keolahragaan dibuktikan dengan occurrences sebanyak 27 kali dan links sebanyak 14 kata. Ide penelitian yang dapat menjadi referensi untuk future research kajian sport recovery dapat berhubungan dengan penelitian tentang Delayed Onset Muscle Soreness (DOMS), pengaruh sport massage dengan sport recovery, tentang stretching PNF, hydration, nutrition, sleep, heat atau thermotherapy, cold atau cryotherapy, contrast bath, oxygenotherapy, aerotherapy, reflexotherapy, chemotherapy, altitude cure dan recovery secara psikologis.
Анализ динамики эпидемического процесса COVID-19 в мире за неделю с 23.02.2024 г. по 01.03.2024 г.
Authors: Зимирова Анастасия , Поспелов Михаил , Иванова Александра , Дмитриева Людмила , Чумачкова Елена , Карнаухов Игорь , Щербакова Светлана , Кутырев Владимир
Published: March 2024

В обзоре представлен анализ эпидемиологической ситуации в странах мира по COVID-19 за неделю с 23.02.2024 г. по 01.03.2024 г.
El mercado escénico que somos:
Authors: Vásquez Solórzano Olga Elena
Published: March 2024

Estudiar el mercado escénico en Colombia ha sido un tema complejo por sus dinámicas particulares. La producción se realiza, por lo general, de manera informal; es difícil tener una excelente experiencia de servicio con las sociedades de gestión colectiva; los ámbitos de circulación no están definidos y, entre otras, se desconoce el comportamiento de los espectadores respecto a la oferta para estimular la demanda. Este artículo es un caso de estudio de los aprendizajes validados del operador de boletería Salallena.com, un emprendimiento que operó desde el 2017 hasta el 2021, fortaleciendo tres eslabones de la cadena de valor: la producción, la exhibición y el mercado. Abrimos nuestra base de datos con más de 36 000 registros y realizamos una lectura cuidadosa de la bibliografía sobre la estructura de mercado de las artes escénicas y producción teatral, con el objetivo de analizar la oferta, los intermediarios, la circulación, la demanda y la contribución parafiscal en Colombia, para dar a conocer otras dinámicas distintas a las que propone la ley de espectáculos públicos.
Multimodal Speaker Recognition using voice and lip movement with decision and feature level fusion
Authors: Nainan Sumita , Kulkarni Vaishali Hemant
Published: March 2024

The speech generation mechanism is fundamentally bimodal in nature. It is an audio and visual representation. Including visual information obtained from the lip movement of a speaker, in addition to the voice is hence justified for a text independent automatic speaker recognition system (ASR). Additionally, lip movement information is invariant to acoustic noise perturbation making the system more robust. Hence, we were motivated to design a dynamic audio-visual speaker recognition system. The objective of this research is to identify a speaker from its voice regardless of the spoken content and strengthen the accuracy of recognition. Classical methods and state of art neural networks has been employed to accomplish this. The learning model for voice modality was computed by concatenating dynamic features to the handcrafted features, which were further optimized using Fisher score technique, leading to improvement in speaker recognition. Support Vector Machines (SVM) and Convolution Neural Network (CNN) classifiers gave a competitive accuracy of 94.77%. For extracting information from the lip movement, Histogram of Gradient (HOG) feature detector algorithm was implemented on the image frames obtained from the video. Unique lip movements were was also computed from the mouth region landmark points of Facial Landmarks. Multimodal framework was accomplished by feature level fusion of voice and lip features with CNN as classifier. The significance of the proposed work lies in the novel use of CNN for speech features. The authors have successfully demonstrated that lip movement features help in liveness detection along with automatic speaker recognition (ASR). The proposed method achieves 91.4% testing accuracy in comparison to the state-of-the-art method.
Karyotype and LTR-RTs analysis provide insights into oak genomic evolution
Authors: CAO Rui-Bin , CHEN Ran , LIAO Ke-Xin , LI He , XU Gang-Biao , JIANG Xiao-Long
Published: March 2024

Background: Whole-genome duplication and long terminal repeat retrotransposons (LTR-RTs) amplification in organisms are essential factors that affect speciation, local adaptation, and diversification of organisms. Understanding the karyotype projection and LTR-RTs amplification could contribute to untangling evolutionary history. This study compared the karyotype and LTR-RTs evolution in the genomes of eight oaks, a dominant lineage in Northern Hemisphere forests.
Results: Karyotype projections showed that chromosomal evolution was relatively conservative in oaks, especially on chromosomes 1 and 7. Modern oak chromosomes formed through multiple fusions, fissions, and rearrangements after an ancestral triplication event. Species-specific chromosomal rearrangements revealed fragments preserved through natural selection and adaptive evolution. A total of 441,449 full-length LTR-RTs were identified from eight oak genomes, and the number of LTR-RTs for oaks from section Cyclobalanopsis was larger than in other sections. Recent amplification of the species-specific LTR-RTs lineages resulted in significant variation in the abundance and composition of LTR-RTs among oaks. The LTR-RTs insertion suppresses gene expression, and the suppressed intensity in gene regions was larger than in promoter regions. Some centromere and rearrangement regions indicated high-density peaks of LTR/Copia and LTR/Gypsy. Different centromeric regional repeat units (32, 78, 79 bp) were detected on different Q. glauca chromosomes.
Conclusion: Chromosome fusions and arm exchanges contribute to the formation of oak karyotypes. The composition and abundance of LTR-RTs are affected by its recent amplification. LTR-RTs random retrotransposition suppresses gene expression and is enriched in centromere and chromosomal rearrangement regions. This study provides novel insights into the evolutionary history of oak karyotypes and the organization, amplification, and function of LTR-RTs.
Comparative transcriptomic analysis primarily explores the molecular mechanism of compound eye formation in Neocaridina denticulata sinensis
Authors: Yan Congcong , Wu Zixuan , Liu Yujie , Sun Yuying , Zhang Jiquan
Published: March 2024

Compound eyes formation in decapod crustaceans occurs after the nauplius stage. However, the key genes and regulatory mechanisms of compound eye development during crustacean embryonic development have not yet been clarified. In this study, RNA-seq was used to investigate the gene expression profiles of Neocaridina denticulata sinensis from nauplius to zoea stage. Based on RNA-seq data analysis, the phototransduction and insect hormone biosynthesis pathways were enriched, and molting-related neuropeptides were highly expressed. There was strong cell proliferation in the embryo prior to compound eye development. The formation of the visual system and the hormonal regulation of hatching were the dominant biological events during compound eye development. The functional analysis of DEGs across all four developmental stages showed that cuticle formation, muscle growth and the establishment of immune system occurred from nauplius to zoea stage. Key genes related to eye development were discovered, including those involved in the determination and differentiation of the eye field, eye-color formation, and visual signal transduction. In conclusion, the results increase the understanding of the molecular mechanism of eye formation in crustacean embryonic stage.
ClimResilience, a global climate resilience framework
Authors: BADOLO Mathieu
Published: March 2024

Baground: Climate change direct and indirect impacts on the main development sectors could induce social, economic, environmental, institutional or political regression dynamics in several regions of the world. The set of markers of such dynamics includes social inequalities and disparities accentuation, community conflicts over natural resources increase, climate migration amplification and humanitarian, social, institutional and political crises intensification. A significant and continuous improvement in knowledge, solutions, technologies and decision support tools for resilience is one of the levers to consider for efficient responses to climate change impacts.
Methods: In this article, we describe the Badolo ClimResilience framework, a global framework for innovating climate resilience governance models, plans, practices and solutions. It includes methodological tools, information and solutions vectors, information triangles, matrices and resilience schemes for improving climate resilience actions relevance, efficiency and performance
Results: The main results of this article are ClimResilience approach, information vectors, information triangles, resilience matrices and schemes. The resilience configurations and trajectories matrix is an innovative tool for planning, achieving and evaluating the desired progress in terms of resilience to climate change
Conclusion: Fundamentally, ClimResilience framework is a new scientific decision support tool for a significant improvement in the relevance, efficiency and performance of climate resilience actions.
Biomechanical control of vascular morphogenesis by the surrounding stiffness
Authors: Nishiyama Koichi , Hanada Yasuyuki , Halder Semanti , Haruta Misato , Ogura Shuntaro , Shiraki Yukihiko , Arima Yuichiro , Nakano Sota , Ozeki Yuka , Murohara Akiyoshi , MUROHARA TOYOAKI
Published: March 2024

Sprouting angiogenesis is a form of morphogenesis which expands vascular networks from the preexisting ones in both developmental and postnatal settings. During this process, endothelial cells (ECs) collectively elongate new branches via their directional movement. Almost simultaneously, the vessel lumen develops internally with blood flowing into it. Our recent study has suggested lumen expansion induced by blood inflow to be an inhibitory factor for branch elongation. However, the precise mechanism governing efficient angiogenic branch elongation during lumen development under the influence of blood inflow remains unknown. Herein, we show perivascular stiffening to be a major factor that integrates branch elongation and lumen development during angiogenic morphogenesis. We found that the circumferential expansion seen during lumen development inhibits directional EC movement driving angiogenic branch elongation, as clarified by an approach combining in vitro on-chip reconstitutions and in vivo validation experiments. This process was counter-regulated by perivascular stiffening that prevents excessive lumen expansion. We also found that appropriate collagen-IV (Col-IV) deposition on the vascular basement membrane (VBM) from ECs driven by pericytes accounts for spatiotemporal perivascular stiffening. Our results demonstrate how ECs elongate branches while developing the lumen by properly building the surrounding physical environment in coordination with pericytes during angiogenesis. These findings provide insights enhancing our understanding of principles biomechanically integrating different morphogenetic processes not only in angiogenesis but also other forms of tissue development, and may in addition provide insights that open avenues to novel applications in clinical management as well as regenerative medicine.
Mothers’ perceptions regarding the implementation of skin-to-skin contact for their preterm infants in intensive care units of the North West Province: A qualitative study
Authors: Denge Takalani , Bam Nokwanda Edith , Lubbe Welma , Rakhudu Mahlasela
Published: March 2024

Background
Approximately 15 million preterm infants are born globally and require hospital care. The health consequences are significant, with complications from preterm birth representing the leading cause of death for children under 5 years of age globally. Fortunately, the majority of these deaths can be prevented with proper care, such as skin-to-skin contact (SSC). SSC is one of the four components of kangaroo care (KC) and is also a valued alternative to incubators in low-income countries. SSC enhances infant and maternal wellbeing and requires an educational programme for implementation. Therefore, mothers' perceptions regarding the implementation of SSC in intensive care units (ICUs) can be influenced by their understanding of its benefits, as well as the support and education provided by healthcare professionals. The purpose of this study was to explore and describe mothers’ perceptions regarding the implementation of SSC for preterm infants in ICUs in Northwest Province (NWP).
Methods
We conducted a qualitative descriptive and exploratory study to describe and explore mothers' perceptions regarding the implementation of SSC for preterm infants in intensive care units of Level 2 hospitals in the NWP from September to November 2023. A total of 24 mothers who had preterm infants admitted to ICUs practicing SSC were included in the Focus Group Discussions (FGDs). Four FGDs consisting of six participants were conducted until data saturation was reached. The data were analyzed thematically using the steps proposed by Creswell and Creswell 2017.
Results
Four main themes were identified, namely, knowledge of SSC, perceived benefits of SSC, challenges experienced by mothers during the implementation of SSC, and support needs for mothers regarding the implementation of SSC, and nine subthemes emerged.
Conclusions
SSC for preterm infants in intensive care units has been found to have positive effects on both mothers and neonates. Mothers perceive SSC as a method of warmth that facilitates bonding and promoting breastfeeding. The findings can inform the programme and preparedness strategies needed to support the implementation of SSC in ICUs.
An efficient topology optimization algorithm for large-scale three-dimensional structures
Authors: Vitorino Alfredo , Gomes Francisco
Published: March 2024

Designing the topology of three-dimensional structures is a challenging problem due to its memory and time consumption. In this paper, we present a robust and efficient algorithm for solving large-scale 3D topology optimization problems. The robustness of the algorithm is ensured by adopting a globally convergent sequen- tial linear programming method with a stopping criterion based on the first-order op- timality conditions of the nonlinear problem. To increase the algorithm’s efficiency, it is combined with a multiresolution scheme that employs different discretizations to deal with displacement, design, and density variables. In addition, the time spent solving the linear equilibrium systems is substantially reduced using multigrid as a preconditioner for the conjugate gradient method. Since multiresolution can lead to the appearance of unwanted artefacts in the structure, we propose an adaptive strategy for increasing the degree of the displacement elements, with a technique for suppress- ing unnecessary variables that provides accurate solutions with a moderate impact on the algorithm’s performance. We also propose a new thresholding strategy, based on gradient information, to obtain structures composed only by solid or void regions. Computational experiments carried out in Matlab prove that the new algorithm effec- tively generates high-resolution structures at a low computational cost.
Shed teeth from Portezuelo Formation at Sierra del Portezuelo reveal a higher diversity of predator theropods during Turonian-Coniacian times in northern Patagonia
Authors: Meso Jorge Gustavo , Gianechini Federico , Gomez Kevin , Muci Luciana , Baiano Mattia Antonio , Pol Diego , Kaluza Jonatan , Garrido Alberto , Pittman Michael
Published: March 2024

The study of thirty-two shed crowns from the Portezuelo Formation (middle Turonian-late Coniacian) at the Sierra del Portezuelo locality, reveals six distinct tooth morphotypes identified through cladistic, discriminant, and cluster analyses. Two morphotypes were identified as belonging to Megaraptoridae, three to Abelisauridae, one to Abelisauroidea, and one to Alvarezsauridae. Additionally, two of the morphotypes exhibit a combination of dental features typically found in megaraptorid and abelisauridtheropods. These results suggest a greater diversity of theropods in the original ecosystem than previously thought, including the presence of a second morphotype of megaraptorid and alvarezsaurid previously undocumented in this formation. Furthermore, the existence of Morphotype 6 indicates the potential coexistence of medium-sized abelisauroids alongside larger abelisaurids in the same ecosystem. These findings underscore the importance of future expeditions to the Sierra del Portezuelo locality to further our understanding of these previously unknown theropod species.
SKSR1 identified as key virulence factor in Cryptosporidium by genetic crossing
Authors: Xiao Lihua , He Wei , Sun Lianbei , Hou Tianyi , Yang Zuiwei , Yang Fuxian , Zhang Shengchen , Wang Tianpeng , Li Na , Guo Yaqiong , Sibley L. , Feng Yaoyu
Published: March 2024

Cryptosporidium is a major cause of severe diarrhea. Although Cryptosporidium isolates exhibit significant differences in infectivity and virulence, the genetic determinants for these traits are not clear. In this study, we used classical genetics to cross two Cryptosporidium parvum isolates of different virulence and used bulked segregant analysis of whole-genome sequence data from the progeny to identify quantitative trait loci (QTL) associated with Cryptosporidium infectivity and virulence. Of the 26 genes in three QTL, two had loss-of-function mutations in the low-virulence isolates. Deletion of the SKSR1 gene or expression of the frame-shift mutant sequence reduced the pathogenicity of infection in vivo. SKSR1 is a polymorphic secretory protein expressed in small granules, secreted into the parasite-host interface, and may cooperate with other secretory proteins in pathogenesis. These results demonstrate that SKSR1 is an important virulence factor in Cryptosporidium, and suggest that the extended SKSR protein family, encoded by variant subtelomeric genes, may contribute to pathogenesis.
In silico investigations of triazole -trimethoxyphenyl hybrids as anti-proliferative agents against adenocarcinomic human alveolar basal epithelial cells (A549): DFT, QSAR and Molecular Docking Approaches
Authors: Sunday Ameh Ojima , Abdullahi Shehu , Gabriel Obiyenwa Kehinde , Moses Osang Ekuli , Banjo Semire
Published: March 2024

Twenty-eight sets of synthesized triazole-trimethoxyphenyl hybrids (TPD) were considered as anti-proliferative drugs against human alveolar basal epithelial (A549) cancer cell lines using DFT, QSAR, ADMET profile and molecular docking methods. The considered compounds were used to develop a robust QSAR model, which was used to design new triazole-trimethoxyphenyl compounds that could serve as anti-proliferative drug candidate against human alveolar basal epithelial (A549) cancer. The parameters obtained from DFT calculations such as the HOMO, LUMO, Dipole moment, chemical hardness and softness favoured TPD-11 and TPD-25 over etopoxide as strong inhibitors against human alveolar basal epithelialcancer cell (A549), which agreed with the experimental data. The QSAR modeling and validation indicated the major influence of MATS4p, minHBint3, and ATSC7c descriptors on the reported anticancer activity of the drugs in the A549- MLR-GFA QSAR (R2 = 0.8632, adjusted R2 = 0.7951, Q2Loo = 0.6023 and R2 - Q2Loo = 0.2609). By leveraging data from the model, four new triazole-trimethoxyphenyl hybrids were proposed (NTPD-3, NTPD-4, NTPD-6 and NTPD-9). The DFT and molecular docking analysis showed these four compounds could be good inhibitors against adenocarcinomic human alveolar basal epithelial cells (A549) than etopoxide. However, ADMET (absorption, distribution, metabolism, excretion, and toxicity) properties revealed NTPD-6 showed excellent pharmacokinetic and toxicological profiles and might serve as a road map for new and more effective anticancer agents.
Assessment of Physico-chemical and Microbiological Characteristics of Water from Lake Dapao, Lanao del Sur, Philippines
Authors: Caneos Warren G. , Macapando Solaiman , Clavano Chelmarie Joy , Abragan Dulce Fe
Published: March 2024

Physico-chemical and microbiological characteristics of water from Lake Dapao, Lanao del Sur, Philippines, were assessed. Water samples were randomly collected from the four established sampling stations in the lake and replicated thrice. In situmeasurements of physico-chemical parameters such as dissolved oxygen, temperature, pH, total dissolved solids (TDS), and water turbidity were done. Water samples were sent to the First Analytical Services and Technical (FAST) Laboratories, Inc., Cagayan de Oro City, for microbiological analysis such as total coliform count, thermotolerant (fecal) coliform count and E. colicount. Results revealed that dissolved oxygen, pH and temperature showed no significant differences between stations, while total dissolved solids and water turbidity showed significant differences between stations. The results of the physico-chemical parameters are considered suitable for fish production, as stated in DAO 2016–08. However, the results of the microbiological tests appeared to have failed to conform to the given water standards as stated in DAO 2017-0010 (PNSDW); thus, the lake waters are not suitable for drinking.
The impact of feeding varied dietary quantities of zinc and copper on turkey poult performance, immunological response and skeletal health
Authors: Agashe Jayanti , Deo Chandra , Biswas Avishek , Sharma Divya , Nampalle Mukesh T , Tiwari Ashok Kumar
Published: March 2024

In this study, the impact of varying dietary zinc (Zn) and copper (Cu) levels on the growth, immunological response, and skeletal health of developing turkey poults was investigated. For 0–4 and 5–8 weeks of age, respectively, three Zn levels of 50, 70, and 90 mg/kg and 45, 65, and 85 mg/kg were employed. Three Cu levels, namely 8, 12, and 16 mg/kg for 0–8 weeks of age, were also utilized. There were 288 (9×4×8) day old turkey poults with equal body weight that were randomly assigned to 9 treatments, each consisting of 4 replicates and 8 poults per replicate. In comparison to other dietary combinations, there was a significant (P ≤ 0.05) increase in body weight gain observed in the 90 and 85 mg Zn/kg with 16 mg Cu/kg diet during 0–4 and 0–8 weeks of age, respectively, and the 70 and 65 mg Zn with 16 mg Cu /kg diet during 0–4 and 0–8 weeks of age, respectively. When compared to low levels of zinc in the diet at 5–8 and 0–8 weeks of age, respectively, feed conversion ratio was shown to be considerably (P ≤ 0.01) lower and better at 90 and 85 mg Zn/kg diet. In a similar pattern, feed utilisation efficiency was considerably (P ≤ 0.01) higher at 16 mg Cu/kg diet than it was at lower Cu levels in the diets given to the animals over the 0–4 week period. Compared to other dietary combinations, there was a substantial (P ≤ 0.01) improvement in cell mediated immune response (foot web index to PHAP) and humoral immune response (HA titer to SRBC) when an 85 mg zinc and 16 mg copper/kg diet was consumed at 0–4 and 5–8 weeks of age, respectively. At greater dietary Zn and Cu levels than its lower values, the weight of the spleen and thymus was considerably higher. In a dietary combination of 90 mg Zn with 16 mg Cu/kg meal during 0–4 and 85 mg Zn with 16 mg Cu/kg diet during 5–8 weeks of age, respectively, there were significantly (P ≤ 0.05) greater bone width (proximal and distal), tibia bone ash, calcium, and phosphorus detected respectively. Significantly (P ≤ 0.01) greater Zn and Fe contents were found in the tibia bone at 90 and 85 mg Zn/kg diet, respectively, compared to values obtained at other Zn levels in the diet throughout 0–4 and 5–8 weeks of age. During the first eight weeks of life, a diet containing 12 mg of copper per kilogramme was shown to have a significantly (P ≤ 0.05) increased Zn and Fe content in the tibia bone compared to other levels. It is possible to draw the conclusion from the data that, for growing turkey poults, dietary combinations of 90 mg Zn/kg with 16 mg Cu/kg diet and 85 mg Zn with 16 mg Cu/kg diet between 0–4 and 5–8 weeks of age, respectively, were sufficient for optimum development, immunity, and skeletal health indices.
Silencing the Shore: The Effects of Onshore Anthropogenic Noise on Marine Mammal Abundances Informed by Mixed Methods
Authors: O'Connor Ryan , Ardoin Nicole , De Leo Giulio
Published: March 2024

We undertook a mixed-methods study to investigate the recent decline in abundance of a colony of Harbor Seals (Phoca vitulina) carefully documented by a community science group in Pacific Grove, California, USA. Combining a generalized additive modeling (GAM) analysis with qualitative analysis of data collected through semi-structured interviews, we analyzed abundance trends between 2003 and 2023, documented Local Ecological Knowledge (LEK) about noisy anthropogenic disturbances and their impact on Harbor Seal behavior and reproductive success, and investigated hypotheses about Harbor Seal abundance trends at the study site. We found that LEK identified a range of potential anthropogenic disturbances whose cumulative effect might explain the decline of the Harbor Seal abundance in recent years. We also found that, in 2022 and 2023, Harbor Seal abundance was 29.81% lower than predicted given the probability of occurrence of forage fish, an important ecological determinant of Harbor Seal abundance. Roadwork construction that occurred in 2022 near the harbor seal colony during the early pupping season may have had significant adverse effects on reproductive success and, consequently, on Harbor Seal abundance at the study site as the “final straw” in an environment of increasing disturbance. This study demonstrates (1) the potential demographic effects of anthropogenic disturbance on marine mammals, (2) the value of mixed-methods design, qualitative interview methods, and community-based science in understanding ecological dynamics in complex social-ecological coastal systems, and (3) the important of mitigating anthropogenic disturbance to sensitive marine mammal colonies.