bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Bacteriophages against Biofilms of Escherichia coli on Food Processing Surfaces
Authors: Brás Ana , Braz Márcia , Martinho Inês , Duarte João , Pereira Carla , Almeida Adelaide
Published: February 2024

The bacterial adhesion to food processing surfaces is a threat to human health, as these surfaces can serve as reservoirs of pathogenic bacteria. Escherichia coli is an easily biofilm-forming bacterium involved in surface contamination that can lead to the cross-contamination of food. Despite the application of disinfection protocols, contamination through food processing surfaces continues to occur. Hence, new, effective, and sustainable alternative approaches are needed. Bacteriophages (or simply phages), viruses that only infect bacteria, have proven to be effective in reducing biofilms. Here, phage phT4A was applied to prevent and reduce E. coli biofilm on plastic and stainless steel surfaces at 25 °C. The biofilm formation capacity of phage-resistant and sensitive bacteria, after treatment, was also evaluated. The inactivation effectiveness of phage phT4A was surface-dependent, showing higher inactivation on plastic surfaces. Maximum reductions in E. coli biofilm of 5.5 and 4.0 log colony-forming units (CFU)/cm2 after 6 h of incubation on plastic and stainless steel, respectively, were observed. In the prevention assays, phage prevented biofilm formation in 3.2 log CFU/cm2 after 12 h. Although the emergence of phage-resistant bacteria has been observed during phage treatment, phage-resistant bacteria had a lower biofilm formation capacity compared to phage-sensitive bacteria. Overall, the results suggest that phages may have applicability as surface disinfectants against pathogenic bacteria, but further studies are needed to validate these findings using phT4A under different environmental conditions and on different materials.
Overview of Ecology and Aspects of Antibiotic Resistance in Campylobacter spp. Isolated from Free-Grazing Chicken Tissues in Rural Households
Authors: Dermatas Argyrios , Rozos Georgios , Zaralis Konstantinos , Dadamogia Aikaterini , Fotou Konstantina , Bezirtzoglou Eugenia , Akrida-Demertzi Konstantoula , Demertzis Panagiotis , Voidarou Chrysoula (Chrysa)
Published: February 2024

Rural households all over the world rear backyard chicken mainly for their own consumption and, to a lesser extent, for barter trade. These chickens represent a staple dish with numerous culinary variations and a cheap source of protein. Although some Campylobacter species, and particularly Campylobacter jejuni and Campylobacter coli, have been associated with industrial poultry carcasses, studies concerning the ecology of this genus in rural households do not exist. To assess the prevalence of Campylobacter species in the tissues of backyard chickens, samples were collected from birds Gallus domesticus bred in households in the rural area of Epirus (Greece), and Campylobacter strains were isolated by quantitative methods at 37 °C and 42 °C. In total, 256 strains were identified, belonging to 17 Campylobacter species, with C. jejuni and C. coli being the most prevalent. From the four ecological parameters studied (size of the flock, presence of small ruminants in the same household, presence of other poultry species in the same household, and feeding leftovers of the household), the size of the flock and the presence of small ruminants and/or pigs in the same household mostly affected the distribution of these strains. To study the phenotypical resistance against 14 antibiotics, 215 strains were selected. The results showed a high prevalence of multidrug-resistance (MDR) strains extending to all classes of antibiotics. Further genome analysis revealed the presence of genes coding resistance (blaOxA-61, tet(O), tet(A) cmeA, cmeB, cmeC, and gyrA (Thr-86-Ile mutation)), with the efflux pump CmeABC being the most prevalent. All antimicrobial resistance-encoded genes co-circulated, except for blaOXA-61, which moved independently. The minimum inhibitory concentration (MIC) values of two out of three antibiotics (representing different classes) were reduced when the strains tested were exposed to carbonyl cyanide 3-chlorophenylhydrazone (CCCP), a known efflux pump inhibitor. The same result was obtained with the addition of CCCP to the MIC values of bile salts. These results lead to the conclusion that Campylobacter species are present in an impressive diversity in backyard chicken tissues and that they exert a significant resistance to antibiotics, raising a potential danger for public health.
Parasitosis by Fasciola hepatica and Variations in Gut Microbiota in School-Aged Children from Peru
Authors: Silva-Caso Wilmer , Carrillo-Ng Hugo , Aguilar-Luis Miguel Angel , Tarazona-Castro Yordi , Valle Luis J. Del , Tinco-Valdez Carmen , Palomares-Reyes Carlos , Urteaga Numan , Bazán-Mayra Jorge , Valle-Mendoza Juana del
Published: February 2024

(1) Background: Human fascioliasis is considered an endemic and hyper-endemic disease in the Peruvian Andean valleys. Our objective was to determine variations in the composition of the gut microbiota among children with Fasciola hepatica and children who do not have this parasitosis. (2) Method: A secondary analysis was performed using fecal samples stored in our biobank. The samples were collected as part of an epidemiological Fasciola hepatica cross-sectional study in children from 4 through 14 years old from a community in Cajamarca, Peru. (3) Results: In a comparison of the bacterial genera that make up the intestinal microbiota between the F. hepatica positive and negative groups, it was found that there are significant differences in the determination of Lactobacillus (p = 0.010, CI: 8.5–61.4), Bacteroides (p = 0.020, CI: 18.5–61.4), Clostridium (p < 0.001, CI: 3.5–36.0), and Bifidobacterium (p = 0.018, CI: 1.1–28.3), with each of these genera being less frequent in children parasitized with F. hepatica. (4) Conclusions: These results show that F. hepatica may be associated with direct or indirect changes in the bacterial population of the intestinal microbiota, particularly affecting three bacterial genera.
Hardware and Software Solutions for the Generation of a Database of HSV-Color Characteristics for the Main Ores and Rocks of the Khibiny Massif
Authors: Shibaeva Darya Nikolaevna , Voronin Roman Pavlovich , Kompanchenko Alena Arkadievna , Volkov Denis Olegovich , Asanovich Danil Alekseevich , Bulatov Victor Vladimirovich
Published: February 2024

This article presents developed hardware and software solutions based on the application of machine vision technology. The hardware and software solutions were created in order to generate a database of HSV-color value for the main ore types, host rocks, and minerals to define criteria for the in-process identification of the Khibiny apatite in testing the walls of blasting boreholes. The hardware ensures a multi-parametric assessment of the optical characteristics of samples and minerals located on their surface. The designed software solution allows the user to control the measurement process; systematize a description of the textural and structural features of the sample under study; and process images of the core surface. The resultant database of HSV-color value for the main ores and rocks of the Khibiny massif and their constituent minerals will provide an opportunity to search for criteria for the in-process identification of the Khibiny apatite in a mineral mixture.
Chemical and Textural Peculiarities of Zircon from Peralkaline Granites and Quartz-Bearing Syenites
Authors: Breiter Karel , Kynický Jindřich , Korbelová Zuzana
Published: February 2024

Zircon from four plutons of peralkaline granites and quartz-bearing syenites, differing in geotectonic positions, petrological and mineralogical compositions, and contents of volatile and trace elements, was studied using SEM, CL, and EPMA with the intention to define typical textural and chemical features of zircon from peralkaline rocks. In strongly peralkaline Na-pyroxene-bearing rocks represented by the Khan Bogd and Khalzan Buregte plutons (Mongolia), the primary zircon is scarce or missing. Most zircon grains are secondary, originating in hydrothermal stage from primary Zr silicates. They often form globular or radial aggregates. Chemical compositions of zircon in these rocks typically show high contents of Y, moderate contents of REE (thus high Y/Yb values) together with low contents of U and Th and low analytical totals. In mildly peralkaline mica-bearing rocks represented by Ivigtut stock (Groenland) and Madeira pluton (Brazil), the exclusive primary Zr mineral is zircon, mostly of orthomagmatic origin. Its analytical totals approach 100 wt%, enrichment in HREE, resulting in low Y/Yb values, is typical. Zircon populations from two types of peralkaline granitoids can be distinguished from each other and from zircon from S-type granites based on combination of the Zr/Hf, Y/Yb, and U/Th values, or on the Y-Hf-P ternary diagram.
Iron–Titanium Oxide–Apatite–Sulfide–Sulfate Microinclusions in Gabbro and Adakite from the Russian Far East Indicate Possible Magmatic Links to Iron Oxide–Apatite and Iron Oxide–Copper–Gold Deposits
Authors: Kepezhinskas Pavel , Berdnikov Nikolai , Krutikova Valeria , Kozhemyako Nadezhda
Published: February 2024

Mesozoic gabbro from the Stanovoy convergent margin and adakitic dacite lava from the Pliocene–Quaternary Bakening volcano in Kamchatka contain iron–titanium oxide–apatite–sulfide–sulfate (ITOASS) microinclusions along with abundant isolated iron–titanium minerals, sulfides and halides of base and precious metals. Iron–titanium minerals include magnetite, ilmenite and rutile; sulfides include chalcopyrite, pyrite and pyrrhotite; sulfates are represented by barite; and halides are predominantly composed of copper and silver chlorides. Apatite in both gabbro and adakitic dacite frequently contains elevated chlorine concentrations (up to 1.7 wt.%). Mineral thermobarometry suggests that the ITOASS microinclusions and associated Fe-Ti minerals and sulfides crystallized from subduction-related metal-rich melts in mid-crustal magmatic conduits at depths of 10 to 20 km below the surface under almost neutral redox conditions (from the unit below to the unit above the QFM buffer). The ITOASS microinclusions in gabbro and adakite from the Russian Far East provide possible magmatic links to iron oxide–apatite (IOA) and iron oxide–copper–gold (IOCG) deposits and offer valuable insights into the early magmatic (pre-metasomatic) evolution of the IOA and ICOG mineralized systems in paleo-subduction- and collision-related geodynamic environments.
Skarn Formation and Zn–Cu Mineralization in the Dachang Sn Polymetallic Ore Field, Guangxi: Insights from Skarn Rock Assemblage and Geochemistry
Authors: He Lei , Liang Ting , Wang Denghong , Zhang Jianxin , Liu Bosheng
Published: February 2024

The Dachang is a world-class, super-giant Sn polymetallic ore field mainly composed of Zn–Cu ore bodies proximal to the granitic pluton and Sn polymetallic ore bodies distal to the granitic pluton. In this study, we used petrographic studies and major and trace element geochemistry with calc-silicates from the Zn–Cu ore bodies to constrain the physicochemical conditions of hydrothermal fluids during skarn rock formation and the evolution of ore-forming elements. Two skarn stages were identified based on petrographic observations: Prograde skarn rocks (Stage I), containing garnet, vesuvianite, pyroxene, wollastonite, and retrograde skarn rocks (Stage II), containing axinite, actinolite, epidote, and chlorite. The retrograde skarn rocks are closely associated with mineralization. The geochemical results show that the garnets in the Dachang ore field belong to the grossular–andradite solid solution, in which the early generation of garnet is mainly composed of grossular (average Gro72And25), while the later generation of garnet is mainly composed of andradite (average Gro39And59); the vesuvianites are Al-rich vesuvianites; the pyroxenes form a diopside–hedenbergite solid solution with a composition of Di3–86Hd14–96; the axinites are mainly composed of ferroaxinite; and the actinolites are Fe-actinolite. The mineral assemblage of the skarn rocks indicates that the ore-forming fluid was in a relatively reduced state in the early prograde skarn stage. As the ore-forming fluid evolved, the oxygen fugacity of the ore-forming fluid increased. During the final skarn stage, the ore-forming fluid changed from a relatively oxidized state to a reduced state. The skarn rocks have evolved from early Al-rich to late Fe-rich characteristics, indicating that the early ore-forming fluid was mainly magmatic exsolution fluid, which may mainly reflect the characteristics of magmatic fluids, and the late Fe-rich characteristics of the skarn rocks may indicate that the late hydrothermal fluid was strongly influenced by country rocks. Trace element analyses showed that the Sn content decreased from the prograde skarn stage to the retrograde skarn stage, indicating that Sn mineralization was not achieved by activating and extracting Sn from prograde skarn rocks by hydrothermal fluids. The significant enrichment of Sn in the magmatic hydrothermal fluid is a necessary condition for Sn mineralization. There are various volatile-rich minerals such as axinite, vesuvianite, fluorite, and tourmaline in the Dachang ore field, indicating that the ore-forming fluid contained extensive volatiles B and F, which may be the fundamental reason for the large-scale mineralization of the Dachang ore field.
From the Mountain and the Sea: Provenance of the Stones of the Prehistoric La Pastora Tholos (Valencina de la Concepción, Seville, Spain)
Authors: Cáceres Luis Miguel , Donaire Teodosio , Ramírez-Cruzado Samuel , Vargas Juan Manuel , Muñiz Fernando , Martín Mónica , Rodríguez-Vidal Joaquín , Ruiz Francisco , Sanjuán Leonardo García
Published: February 2024

La Pastora tholos is the most complete and characteristic megalithic monument of the Valencina de la Concepción-Castilleja de Guzmán mega-site. This monument was built using three types of rock, each with different functions and coming from three different places: Paleozoic quartzarenite, granite, and Tertiary calcareous sandstone. A detailed petrological study of the rocks of the tholos and outcrops of similar rocks in the surroundings has been carried out, locating the possible source areas in areas at least 30 km to the N of the monument for the quartzarenite, 15 km for the granite, and 15 km to the S for the sandstone. Therefore, the community that built the monument has a high knowledge of the nature by, which allows them to locate these resources, and a sufficient social organization to exploit them and move them to the mega-site. In this sense, the layout of the outcrops suggests the possible use of boats or rafts to facilitate their displacement, or at least part of their movement.
Phytochemical Profile and In Vitro Bioactivities of Wild Asparagus stipularis
Authors: Hamdi Amel , Jaramillo-Carmona Sara , Rodríguez-Arcos Rocío , Jiménez-Araujo Ana , Karray Bouraoui Najoua , Guillén-Bejarano Rafael
Published: February 2024

In this study, Asparagus stipularis was characterized concerning its phytochemical composition, antioxidant potential, cytotoxicity, and pancreatic lipase inhibitory activities. Twenty-seven compounds were identified and quantified by HPLC-DAD-MS in the leaf, stem, pericarp, and rhizome of ethanolic extracts. Seven steroidal saponins were detected, and the highest content was quantified in rhizome and pericap. A. stipularis also contained significant amounts of flavonoids in the aerial part. Isorhamnetin tetra-glycoside, quercetin-3-glucosyl-rutinoside, and rutin were the main flavonoid derivatives in leaf, stem, and pericarp extracts, respectively. In addition, eleven phenolic acids were also detected; among them, caffeic acid, protocatechuic acid, p-hydroxybenzoic acid, and ferulic acid were the predominant phenolics, with these having the highest amounts quantified in the rhizome extracts. All the tested extracts possessed antioxidant capacities, with pericarp and rhizome extracts exhibiting the highest activity in DPPH, ABTS, and FRAP assays. The extracts from pericarp and rhizome were revealed to also be the strongest inhibitors of pancreatic lipase. The rhizome extracts exhibited potent cytotoxic activity against HCT-116 and HepG2 with IC50 values of 30 and 54 µg/mL after 48 h of treatment. The present study demonstrated that A. stipularis can be used as a new source of natural antioxidants and potential anticancer and antiobesity compounds.
Computer-Aided Strategy on 5-(Substituted benzylidene) Thiazolidine-2,4-Diones to Develop New and Potent PTP1B Inhibitors: QSAR Modeling, Molecular Docking, Molecular Dynamics, PASS Predictions, and DFT Investigations
Authors: Derki Nour-El Houda , Kerassa Aicha , Belaidi Salah , Derki Maroua , Yamari Imane , Samadi Abdelouahid , Chtita Samir
Published: February 2024

A set of 5-(substituted benzylidene) thiazolidine-2,4-dione derivatives was explored to study the main structural requirement for the design of protein tyrosine phosphatase 1B (PTP1B) inhibitors. Utilizing multiple linear regression (MLR) analysis, we constructed a robust quantitative structure–activity relationship (QSAR) model to predict inhibitory activity, resulting in a noteworthy correlation coefficient (R2) of 0.942. Rigorous cross-validation using the leave-one-out (LOO) technique and statistical parameter calculations affirmed the model’s reliability, with the QSAR analysis revealing 10 distinct structural patterns influencing PTP1B inhibitory activity. Compound 7e(ref) emerged as the optimal scaffold for drug design. Seven new PTP1B inhibitors were designed based on the QSAR model, followed by molecular docking studies to predict interactions and identify structural features. Pharmacokinetics properties were assessed through drug-likeness and ADMET studies. After that density functional theory (DFT) was conducted to assess the stability and reactivity of potential diabetes mellitus drug candidates. The subsequent dynamic simulation phase provided additional insights into stability and interactions dynamics of the top-ranked compound 11c. This comprehensive approach enhances our understanding of potential drug candidates for treating diabetes mellitus.
Fluorescent Sensor Based on 1H-Pyrazolo[3,4-b]quinoline Derivative for Detecting Zn2+ Cations
Authors: Kolbus Anna , Uchacz Tomasz , Danel Andrzej , Gałczyńska Katarzyna , Moskwa Paulina , Kolek Przemysław
Published: February 2024

The photophysical and sensory properties of the donor–acceptor pyrazoloquinoline derivative (PQPc) were investigated using absorption, steady-state, and time-resolved fluorescence measurements. The compound synthesized from commercial, readily available substrates exhibited absorptions in the UV–Vis range, with a maximum of the longwave band around 390 nm. The maximum fluorescence was around 460–480 nm, depending on the solvent. The quantum yield was between 12.87% (for n-hexane) and 0.75% (for acetonitrile) and decreased with increasing solvent polarity. The PET mechanism was implicated as the cause of fluorescence quenching. Divalent ions such as Zn2+, Pb2+, Cd2+, Ca2+, Mg2+, Co2+, Ni2+, and Cu2+ were introduced to study the fluorescent response of PQPc. A 13-times increase in fluorescence quantum yield was observed after the addition of Zn2+ ions. Detailed research was carried out for the PQPc-Zn2+ system in order to check the possibility of analytical applications of PQPc as a fluorescent sensor. A detection limit of Zn2+ was set at the value level 1.93 × 10−7 M. PQPc-Zn2+ complexes had a stoichiometry of 1:1 with a binding constant of 859 M−1. Biological studies showed that the sensor was localized in cells near the membrane and cytoplasm and may be used to detect zinc ions in eukaryotic cells.
Anticancer Metallocenes and Metal Complexes of Transition Elements from Groups 4 to 7
Authors: Kostova Irena
Published: February 2024

With the progression in the field of bioinorganic chemistry, the role of transition metal complexes as the most widely used therapeutics is becoming a more and more attractive research area. The complexes of transition metals possess a great variety of attractive pharmacological properties, including anticancer, anti-inflammatory, antioxidant, anti-infective, etc., activities. Transition metal complexes have proven to be potential alternatives to biologically active organic compounds, especially as antitumor agents. The performance of metal coordination compounds in living systems is anticipated to differ generally from the action of non-metal-containing drugs and may offer unique diagnostic and/or therapeutic opportunities. In this review, the rapid development and application of metallocenes and metal complexes of elements from Groups 4 to 7 in cancer diagnostics and therapy have been summarized. Most of the heavy metals discussed in the current review are newly discovered metals. That is why the use of their metal-based compounds has attracted a lot of attention concerning their organometallic and coordination chemistry. All of this imposes more systematic studies on their biological activity, biocompatibility, and toxicity and presupposes further investigations.
Water Leaching Kinetics of Boron from the Alkali-Activated Ludwigite Ore
Authors: Liang Binjun , Hu Haixiang , Xiao Bin , Lu Zhigang , Yuan Weiquan , Huang Zheyu
Published: February 2024

The primary aim of this study was to investigate the boron leaching process from alkali-activated ludwigite ore. Initially, the ore underwent activation through roasting at 1050 °C for 60 min with 20% sodium carbonate. Subsequently, the study examined the influence of leaching parameters, including temperature, time, liquid-to-solid ratio, and particle size, using the activated ore as the raw material. Additionally, water leaching characteristics of the residues and boron kinetics were analyzed. The results demonstrated that boron leaching efficiency reached 93.71% from the reduced ludwigite ore under specific conditions: leaching temperature of 180 °C, leaching time of 6 h, liquid-to-solid ratio of 8:1, and feed particle size of 52.31 μm (average particle size). Leach residue characteristics indicated the dissolution of minerals during the process. The boron behavior during water leaching followed the Avrami Equation, and the kinetics equation was derived by fitting the leaching data. Moreover, the activation energy (Ea) value for boron leaching was determined to be 8.812 kJ·mol−1 using the Arrhenius Equation, indicating that the leaching process is controlled by diffusion.
LiNbO3 Thin Films through a Sol–Gel/Spin-Coating Approach Using a Novel Heterobimetallic Lithium–Niobium Precursor
Authors: Lo Presti Francesca , Pellegrino Anna Lucia , Micard Quentin , Condorelli Guglielmo Guido , Margueron Samuel , Bartasyte Ausrine , Malandrino Graziella
Published: February 2024

Lithium niobate is a lead-free material which has attracted considerable attention due to its excellent optical, piezoelectric, and ferroelectric properties. This research is devoted to the synthesis through an innovative sol–gel/spin-coating approach of polycrystalline LiNbO3 films on Si substrates. A novel single-source hetero-bimetallic precursor containing lithium and niobium was synthesized and applied to the sol–gel synthesis. The structural, compositional, and thermal characteristics of the precursor have been tested through attenuated total reflection, X-ray photoelectron spectroscopy, thermogravimetric analysis, and differential scanning calorimetry. The LiNbO3 films have been characterized from a structural point of view with combined X-ray diffraction and Raman spectroscopy. Field-emission scanning electron microscopy, energy dispersive X-ray analysis, and X-ray photoelectron spectroscopy have been used to study the morphological and compositional properties of the deposited films.
Flame Spray Pyrolysis Synthesis of Vo-Rich Nano-SrTiO3-x
Authors: Zindrou Areti , Psathas Pavlos , Deligiannakis Yiannis
Published: February 2024

Engineering of oxygen vacancies (Vo) in nanomaterials allows diligent control of their physicochemical properties. SrTiO3 possesses the typical ABO3 structure and has attracted considerable attention among the titanates due to its chemical stability and its high conduction band energy. This has resulted in its extensive use in photocatalytic energy-related processes, among others. Herein, we introduce the use of Flame Spray Pyrolysis (FSP); an industrial and scalable process to produce Vo-rich SrTiO3 perovskites. We present two types of Anoxic Flame Spray Pyrolysis (A-FSP) technologies using CH4 gas as a reducing source: Radial A-FSP (RA-FSP); and Axial A-FSP (AA-FSP). These are used for the control engineering of oxygen vacancies in the SrTiO3-x nanolattice. Based on X-ray photoelectron spectroscopy, Raman and thermogravimetry-differential thermal analysis, we discuss the role and the amount of the Vos in the so-produced nano-SrTiO3-x, correlating the properties of the nanolattice and energy-band structure of the SrTiO3-x. The present work further corroborates the versatility of FSP as a synthetic process and the potential future application of this process to engineer photocatalysts with oxygen vacancies in quantities that can be measured in kilograms.
Inkjet-Printed Dielectric Layer for the Enhancement of Electrowetting Display Devices
Authors: Jiang Hongwei , Qian Rongzhen , Yang Tinghong , Guo Yuanyuan , Yuan Dong , Tang Biao , Zhou Rui , Li Hui , Zhou Guofu
Published: February 2024

Electrowetting with a dielectric layer is commonly preferred in practical applications. However, its potential is often limited by factors like the properties of the dielectric layer and its breakdown, along with the complexity of the deposition method. Fortunately, advancements in 3D inkjet printing offer a more adaptable solution for making patterned functional layers. In this study, we used a negative photoresist (HN-1901) to create a new dielectric layer for an electrowetting display on a 3-inch ITO glass using a Dimatix DMP-2580 inkjet printer. The resulting devices performed better due to their enhanced resistance to dielectric breakdown. We meticulously investigated the physical properties of the photoresist material and printer settings to achieve optimal printing. We also controlled the uniformity of the dielectric layer by adjusting ink drop spacing. Compared to traditional electrowetting display devices, those with inkjet-printed dielectric layers showed significantly fewer defects like bubbles and electrode corrosion. They maintained an outstanding response time and breakdown resistance, operating at an open voltage of 20 V. Remarkably, these devices achieved faster response times of ton 22.3 ms and toff 14.2 ms, surpassing the performance of the standard device.
A Hierarchical Security Event Correlation Model for Real-Time Threat Detection and Response
Authors: Maosa Herbert , Ouazzane Karim , Ghanem Mohamed Chahine
Published: February 2024

An intrusion detection system (IDS) perform postcompromise detection of security breaches whenever preventive measures such as firewalls do not avert an attack. However, these systems raise a vast number of alerts that must be analyzed and triaged by security analysts. This process is largely manual, tedious, and time-consuming. Alert correlation is a technique that reduces the number of intrusion alerts by aggregating alerts that are similar in some way. However, the correlation is performed outside the IDS through third-party systems and tools, after the IDS has already generated a high volume of alerts. These third-party systems add to the complexity of security operations. In this paper, we build on the highly researched area of alert and event correlation by developing a novel hierarchical event correlation model that promises to reduce the number of alerts issued by an intrusion detection system. This is achieved by correlating the events before the IDS classifies them. The proposed model takes the best features from similarity and graph-based correlation techniques to deliver an ensemble capability not possible by either approach separately. Further, we propose a correlation process for events rather than alerts as is the case in the current art. We further develop our own correlation and clustering algorithm which is tailor-made to the correlation and clustering of network event data. The model is implemented as a proof of concept with experiments run on standard intrusion detection sets. The correlation achieves an 87% data reduction through aggregation, producing nearly 21,000 clusters in about 30 s.
Adapting the Dietary Fat and Free Sugar Short Questionnaire: A Comprehensive Polish Modification for Enhanced Precision in Nutritional Assessments
Authors: Tabiś Katarzyna , Maćków Monika , Nowacki Dorian , Poprawa Ryszard
Published: February 2024

Background: This study aimed to investigate whether The Dietary Fat and Free Sugar—Short Questionnaire (DFS) is a reliable and valid measure that can be used in Polish conditions. It involved 291 participants, aged 14 to 70 (M = 25.9, SD = 10.1), the majority of whom were women (75%). Methods: The questionnaire consisted of, among others, Polish DFS version, FFQ-6, TFEQ, and SCOFF. Test–retest reliability was established on the group of 26 students with a 2-week interval. Participants’ percentage of energy intake from both free sugars and saturated fats based on FFQ was correlated with DFS total and subscales scores. To investigate convergent and divergent validity, DFS scores with TFEQ correlations were performed. Diagnostic validity was established based on difference analysis between groups with the risk of eating disorders and those without the risk of those based on SCOFF. Results: Test–retest reliability (rtr = 0.856) and internal consistency (α = 0.797) indicated excellent reliability. DFS correlated significantly with FFQ for both total scores (r = 0.82) and each subscale: sugar (0.79), fat (0.75), and fat–sugar (0.59). The correlations of DFS and TFEQ were statistically significant for cognitive restraint (r = 0.32) and uncontrolled eating (r = 0.19). There also have been found significant differences based on SCOFF regarding DFS. Conclusions: The results suggest good reliability and validity of the Polish version of DFS.
Targeting Metabolic Diseases: The Role of Nutraceuticals in Modulating Oxidative Stress and Inflammation
Authors: Dama Aida , Shpati Kleva , Daliu Patricia , Dumur Seyma , Gorica Era , Santini Antonello
Published: February 2024

The escalating prevalence of metabolic and cardiometabolic disorders, often characterized by oxidative stress and chronic inflammation, poses significant health challenges globally. As the traditional therapeutic approaches may sometimes fall short in managing these health conditions, attention is growing toward nutraceuticals worldwide; with compounds being obtained from natural sources with potential therapeutic beneficial effects being shown to potentially support and, in some cases, replace pharmacological treatments, especially for individuals who do not qualify for conventional pharmacological treatments. This review delves into the burgeoning field of nutraceutical-based pharmacological modulation as a promising strategy for attenuating oxidative stress and inflammation in metabolic and cardiometabolic disorders. Drawing from an extensive body of research, the review showcases various nutraceutical agents, such as polyphenols, omega-3 fatty acids, and antioxidants, which exhibit antioxidative and anti-inflammatory properties. All these can be classified as novel nutraceutical-based drugs that are capable of regulating pathways to mitigate oxidative-stress- and inflammation-associated metabolic diseases. By exploring the mechanisms through which nutraceuticals interact with oxidative stress pathways and immune responses, this review highlights their potential to restore redox balance and temper chronic inflammation. Additionally, the challenges and prospects of nutraceutical-based interventions are discussed, encompassing bioavailability enhancement, personalized treatment approaches, and clinical translation. Through a comprehensive analysis of the latest scientific reports, this article underscores the potential of nutraceutical-based pharmacological treatment modulation as a novel avenue to fight oxidative stress and inflammation in the complex landscape of metabolic disorders, particularly accentuating their impact on cardiovascular health.
The Nutritional Quality of Food Donated to a Western Australian Food Bank
Authors: Mossenson Sharonna , Giglia Roslyn , Pulker Claire E. , Dhaliwal Satvinder S. , Chester Miranda , Bigwood Ruby , Pollard Christina M.
Published: February 2024

Food banks provide an indispensable service to people experiencing severe food insecurity. Food banks source donations from across the food system; however, the food redistributed to clients across the developed world is nutritionally poor. This, together with the increasing prevalence of diet-related diseases and food insecurity, has prompted a focus on nutritional quality. Despite more food being distributed via food banks in Australia, the nutritional quality of donated food remains unreported. This study analyzed all food (84,996 kg (1216 products)) donated to Foodbank WA over a 5-day period using diet-, food-, and nutrient-based nutrition classification schemes (NCSs). A total of 42% (27% of total weight) of donated food products were deemed ‘unsuitable’ and 19% (23% by weight) were ‘suitable’ according to all NCSs. There was no agreement on 39% of products (50% by weight). Overall, NOVA and the Healthy Eating Research Nutrition Guidelines (HERNG) (κ = 0.521) had the highest level of agreement and the ADGs and HERNGs the lowest (κ = 0.329). The findings confirm the poor nutritional quality of food donated to food banks and the need to work with donors to improve the food they donate. Fit-for-purpose nutrition guidelines are urgently needed for Australian food banks to support them in providing nutritious food to their vulnerable clients.
Sixty Years of Innovation in Biomechanical Orthognathic Surgery: The State of the Art and Future Directions
Authors: Andreucci Carlos Aurelio
Published: February 2024

Craniofacial surgery is proposed and performed for a variety of reasons, ranging from congenital or acquired malformations to emotional disorders and parafunctions of the masticatory, respiratory, auditory, and visual systems. Surgery of the mandible and its orthostatic repositioning is the most common of these corrections of craniofacial anomalies. Throughout the history of these procedures, various techniques have been proposed and perfected, but always with a high rate of minor and major complications. The recurrence rate of mandibular malposition is high, as is the temporary loss of facial sensitivity and motor skills. These outcomes are often related to the choice of surgical technique rather than the skill of the surgeon, which is considered to be one of the most important factors in the final outcome. Surgical techniques involving direct manipulation of the vascular-nervous bundles, such as bilateral sagittal split osteotomy, clearly present the possibility of major or minor complications. In this study, an orthognathic surgical technique, performed by the same team for over 40 years and now available through a 20-year postoperative patient follow-up study, is presented with a literature review relating it to biomechanical concepts and bone remodeling to analyze the evolution of orthognathic surgery since it became common practice to correct maxillofacial discrepancies. In this review, we also present a case report in which previous orthodontic treatment prepared a patient for surgical correction of mandibular bone discrepancy without the need for combined maxillary and/or genioplasty, and we describe the most commonly used techniques today, as well as their advantages and disadvantages. The combination of established concepts together promotes favorable stability of mandibular osteotomies, functional anatomical positioning of the temporomandibular joint, reduced risk of injury to the mandibular vasculo-nervous bundle, and good aesthetics with positive patient acceptance and no relapse, thus these are the objectives for proposing innovative treatments that combine the technologies available today.
Extraction of Bioactive Compounds from Cistus creticus Leaves and Their Use in the Preparation of Yogurt Desserts
Authors: Palaiogiannis Dimitrios , Athanasiadis Vassilis , Chatzimitakos Theodoros , Mantiniotou Martha , Bozinou Eleni , Makris Dimitris P. , Lalas Stavros I.
Published: February 2024

The objective of this study was to determine the optimal conditions for the recovery of bioactive and antioxidant compounds in aqueous solutions of Cistus creticus leaves and then employ the optimal extract for the enrichment of yogurt samples. The optimal conditions were established by a response surface methodology and were determined to be a liquid-to-solid ratio of 48 mL/g at 76 °C for 41 min. The optimum extract yielded TPC 157.17 mg GAE/g dw and TFC 2.38 mg QE/g dw, while FRAP and DPPH values were 1258.52 and 933.67 μmol AAE/g dw, respectively. HPLC-DAD was utilized to identify and quantify specific polyphenols, like myricetin rhamnoside, in the extract. The optimal extract was then added to yogurt desserts during their preparation at three different concentrations to study how the physicochemical characteristics of the yogurt, as well as the antioxidant capacity added during enrichment, were affected. Statistical analysis of the results was carried out in order to obtain more valid data. It seems that the most suitable concentration for yogurt fortification was 0.1% w/v of the extract as, at this concentration, the yogurts exhibited higher antioxidant capacity, and their physicochemical characteristics were improved.
Evaluation of Inactivation Methods for Rift Valley Fever Virus in Mouse Microglia
Authors: Rangel Margarita V. , Bourguet Feliza A. , Hall Carolyn I. , Weilhammer Dina R.
Published: February 2024

Rift Valley fever phlebovirus (RVFV) is a highly pathogenic mosquito-borne virus with bioweapon potential due to its ability to be spread by aerosol transmission. Neurological symptoms are among the worst outcomes of infection, and understanding of pathogenesis mechanisms within the brain is limited. RVFV is classified as an overlap select agent by the CDC and USDA; therefore, experiments involving fully virulent strains of virus are tightly regulated. Here, we present two methods for inactivation of live virus within samples derived from mouse microglia cells using commercially available kits for the preparation of cells for flow cytometry and RNA extraction. Using the flow cytometry protocol, we demonstrate key differences in the response of primary murine microglia to infection with fully virulent versus attenuated RVFV.
In Vitro Sensitivity Test of Fusarium Species from Weeds and Non-Gramineous Plants to Triazole Fungicides
Authors: Matelionienė Neringa , Žvirdauskienė Renata , Kadžienė Gražina , Zavtrikovienė Evelina , Supronienė Skaidrė
Published: February 2024

Fusarium species are common plant pathogens that cause serious crop losses worldwide. Fusarium spp. colonize not only the main host plants, crops, but also alternative hosts. The effectiveness of fungicide use in disease management ranges from very successful to possibly promoting the growth of the pathogen. Triazole fungicides are widely used to control these pathogens due to their broad-spectrum activity and systemic nature. This paper reviews the sensitivity of 40 Fusarium strains isolated from weeds, non-gramineous plants, and spring wheat to metconazole, prothioconazole, and tebuconazole. The effect of fungicides was determined by the percentage inhibition of F. graminearum, F. culmorum, F. sporotrichioides, and F. avenaceum fungal mycelial growth. The 50% effective concentration (EC50) values of all isolates on metconazole were lower than 2.9 mg L−1, prothioconazole EC50 ranged from 0.12 to 23.6 mg L−1, and tebuconazole ranged from 0.09 to 15.6 mg L−1. At 0.00025–0.025 mg L−1, the fungicides were ineffective, except for the growth of the F. avenaceum species. It was observed that isolates from weeds were more sensitive to low concentrations of fungicide than isolates from crop plants. In general, information is scarce regarding the comparison of fungicide resistance in Fusarium isolates from weed and crop plants, making this study an additional contribution to the existing knowledge base.
Pseudopropionibacterium propionicum as a Cause of Empyema; A Diagnosis with Next-Generation Sequencing
Authors: Babar Sumbal , Liu Emily , Kaur Savreet , Hussain Juzar , Danaher Patrick J. , Anstead Gregory M.
Published: February 2024

Pseudopropionibacterium propionicum (P.p.) is an anaerobic, Gram-positive, branching beaded rod that is a component of the human microbiome. An infection of the thoracic cavity with P.p. can mimic tuberculosis (TB), nocardiosis, and malignancy. We present a case of a 77-year-old male who presented with dyspnea and a productive cough who was initially misdiagnosed with TB based on positive acid-fast staining of a pleural biopsy specimen and an elevated adenosine deaminase level of the pleural fluid. He was then diagnosed with nocardiosis based on the Gram stain of his pleural fluid that showed a Gram-positive beaded and branching rod. The pleural fluid specimen was culture-negative, but the diagnosis of thoracic P.p. infection was determined with next-generation sequencing (NGS). The patient was initially treated with imipenem and minocycline, then ceftriaxone and minocycline, and later changed to minocycline only. This report shows the utility of NGS in making a microbiological diagnosis when other techniques either failed to provide a result (culture) or gave misleading information (histopathologic exam, pleural fluid adenosine deaminase determination, and organism morphology on Gram stain).
OsbZIP18 Is a Positive Regulator of Phenylpropanoid and Flavonoid Biosynthesis under UV-B Radiation in Rice
Authors: Liu Xueqing , Xie Ziyang , Xin Jiajun , Yuan Shiqing , Liu Shuo , Sun Yangyang , Zhang Yuanyuan , Jin Cheng
Published: February 2024

In plants exposed to ultraviolet B radiation (UV-B; 280–315 nm), metabolic responses are activated, which reduce the damage caused by UV-B. Although several metabolites responding to UV-B stress have been identified in plants, the accumulation of these metabolites at different time points under UV-B stress remains largely unclear, and the transcription factors regulating these metabolites have not been well characterized. Here, we explored the changes in metabolites in rice after UV-B treatment for 0 h, 6 h, 12 h, and 24 h and identified six patterns of metabolic change. We show that the rice transcription factor OsbZIP18 plays an important role in regulating phenylpropanoid and flavonoid biosynthesis under UV-B stress in rice. Metabolic profiling revealed that the contents of phenylpropanoid and flavonoid were significantly reduced in osbzip18 mutants compared with the wild-type plants (WT) under UV-B stress. Further analysis showed that the expression of many genes involved in the phenylpropanoid and flavonoid biosynthesis pathways was lower in osbzip18 mutants than in WT plants, including OsPAL5, OsC4H, Os4CL, OsCHS, OsCHIL2, and OsF3H. Electrophoretic mobility shift assays (EMSA) revealed that OsbZIP18 bind to the promoters of these genes, suggesting that OsbZIP18 function is an important positive regulator of phenylpropanoid and flavonoid biosynthesis under UV-B stress. In conclusion, our findings revealed that OsbZIP18 is an essential regulator for phenylpropanoid and flavonoid biosynthesis and plays a crucial role in regulating UV-B stress responses in rice.
Reduced Fertilization to Improve Sustainable Use of Resources and Preserve Postharvest Quality of Fresh-Cut Wild Rocket (Diplotaxis tenuifolia L.) in Soil-Bound and Soilless Cultivation
Authors: Palumbo Michela , Bonelli Lucia , Pace Bernardo , Montesano Francesco Fabiano , Serio Francesco , Cefola Maria
Published: February 2024

Reducing fertilizer input is a goal for helping greenhouse farming to achieve higher sustainability in the production process while preserving overall crop performance and quality. Wild rocket plants were cultivated in a plastic greenhouse divided into two independent sectors, one for soil-bound (SbS) cultivation and another equipped for soilless (ScS) cultivation systems. In both SbS and ScS, the crop was subjected to treatments consisting of a high- and a low-input fertilization program (HF and LF treatment, respectively). Water use efficiency (WUE) and partial factor productivity (PFP) for nutrients (N, P, K, Ca, and Mg for ScS, and N for SbS) were measured. Rocket leaves, separated for the cultivation system and fertilization program and collected at different cuts during the growing cycle, were cold stored at 10 °C until 16 d. On each sampling day (at harvest and during storage), the sensory parameters, respiration rate, dry matter, color, electrolyte leakage, antioxidant activity, total phenols, total chlorophyll and ammonia content were evaluated. In ScS, the PFP for all nutrients supplied as fertilizers showed a significant increase with the LF treatment, with values higher than 30% recorded for N, K, and Ca. As for the postharvest performance, rocket leaves cultivated in ScS showed better qualitative traits than those cultivated in SbS, as suggested by the lower values of ammonia content and electrolyte leakage recorded at the end of storage period in samples grown in ScS. Moreover, in ScS, the data showed lower membrane damage in LF than HF rocket leaves. Finally, regarding total chlorophyll content, even if no effect of each treatment was recorded in SbS, rocket cultivated in ScS showed a better retention of this parameter by applying LF rather than HF treatment. In addition to this, a PLS model (R2 = 0.7) able to predict the cultivation system, using as a variable non-destructively measured total chlorophyll content, was implemented. Low fertilization input, both in SbS and in ScS, allowed satisfying production levels and more sustainable management of nutrients. LF treatment applied to ScS also had in positive effects on the postharvest quality of fresh-cut rocket leaves.