bluuebunny/crossref_metadata_2025 huggingface dataset:
Neurorrhaphy in Presence of Polyethylene Glycol Enables Immediate Electrophysiological Conduction in Porcine Model of Facial Nerve Injury
Authors: Petrov Dmitriy , Burrell Justin C. , Browne Kevin D. , Laimo Franco A. , Roberts Sanford E. , Ali Zarina S. , Cullen D. Kacy
Published: March 2022

Facial nerve trauma often leads to disfiguring facial muscle paralysis. Despite several promising advancements, facial nerve repair procedures often do not lead to complete functional recovery. Development of novel repair strategies requires testing in relevant preclinical models that replicate key clinical features. Several studies have reported that fusogens, such as polyethylene glycol (PEG), can improve functional recovery by enabling immediate reconnection of injured axons; however, these findings have yet to be demonstrated in a large animal model. We first describe a porcine model of facial nerve injury and repair, including the relevant anatomy, surgical approach, and naive nerve morphometry. Next, we report positive findings from a proof-of-concept experiment testing whether a neurorrhaphy performed in conjunction with a PEG solution maintained electrophysiological nerve conduction at an acute time point in a large animal model. The buccal branch of the facial nerve was transected and then immediately repaired by direct anastomosis and PEG application. Immediate electrical conduction was recorded in the PEG-fused nerves (n = 9/9), whereas no signal was obtained in a control cohort lacking calcium chelating agent in one step (n = 0/3) and in the no PEG control group (n = 0/5). Nerve histology revealed putative-fused axons across the repair site, whereas no positive signal was observed in the controls. Rapid electrophysiological recovery following nerve fusion in a highly translatable porcine model of nerve injury supports previous studies suggesting neurorrhaphy supplemented with PEG may be a promising strategy for severe nerve injury. While acute PEG-mediated axon conduction is promising, additional work is necessary to determine if physical axon fusion occurs and the longer-term fate of distal axon segments as related to functional recovery.
Value of CRP, PCT, and NLR in Prediction of Severity and Prognosis of Patients With Bloodstream Infections and Sepsis
Authors: Liang Peipei , Yu Feng
Published: March 2022

Objective

To investigate the value of C-reactive protein (CRP), procalcitonin (PCT), and neutrophil to lymphocyte ratio (NLR) in assessing the severity of disease in patients with bloodstream infection and sepsis, and to analyze the relationship between the levels of three inflammatory factors and the prognosis of patients.

Methods

The clinical data of 146 patients with bloodstream infection and sepsis admitted to our intensive care unit (ICU) from October 2016 to May 2020 were retrospectively analyzed. The differences in the levels of inflammatory indicators such as CRP, PCT, and NLR within 24 h in patients with bloodstream infection sepsis with different conditions (critical group, non-critical group) and the correlation between these factors and the condition (acute physiology and chronic health evaluation II, APACHE II score) were analyzed. In addition, the prognosis of all patients within 28 days was counted, and the patients were divided into death and survival groups according to their mortality, and the risk factors affecting their death were analyzed by logistic regression, and the receiver operating characteristic (ROC) curve was used to analyze the value of the relevant indicators in assessing the prognosis of patients.

Results

The levels of NLR, CRP, PCT, total bilirubin (TBIL), glutamic oxaloacetic transaminase (AST), and serum creatinine (Scr) were significantly higher in the critically ill group than in the non-critically ill group, where correlation analysis revealed a positive correlation between CRP, PCT, and NLR and APACHE II scores (P < 0.05). Univariate logistic regression analysis revealed that CRP, PCT, NLR, and APACHE II scores were associated with patient prognosis (P < 0.05). Multi-factor logistic regression analysis found that PCT, NLR, and APACHE II scores were independent risk factors for patient mortality within 28 days (P < 0.05). ROC curve analysis found that PCT and NLR both had an AUC area > 0.7 in predicting patient death within 28 days (P < 0.05).

Conclusion

Inflammatory factors such as NLR, CRP, and PCT have important clinical applications in the assessment of the extent of disease and prognosis of patients with bloodstream infection and sepsis.
Model Integration in Computational Biology: The Role of Reproducibility, Credibility and Utility
Authors: Karr Jonathan , Malik-Sheriff Rahuman S. , Osborne James , Gonzalez-Parra Gilberto , Forgoston Eric , Bowness Ruth , Liu Yaling , Thompson Robin , Garira Winston , Barhak Jacob , Rice John , Torres Marcella , Dobrovolny Hana M. , Tang Tingting , Waites William , Glazier James A. , Faeder James R. , Kulesza Alexander
Published: March 2022

During the COVID-19 pandemic, mathematical modeling of disease transmission has become a cornerstone of key state decisions. To advance the state-of-the-art host viral modeling to handle future pandemics, many scientists working on related issues assembled to discuss the topics. These discussions exposed the reproducibility crisis that leads to inability to reuse and integrate models. This document summarizes these discussions, presents difficulties, and mentions existing efforts towards future solutions that will allow future model utility and integration. We argue that without addressing these challenges, scientists will have diminished ability to build, disseminate, and implement high-impact multi-scale modeling that is needed to understand the health crises we face.
Utility of the Intradermal Skin Test in a Test-and-Cull Approach to Control Bovine Tuberculosis: A Pilot Study in Ethiopia
Authors: Lakew Matios , Srinivasan Sreenidhi , Mesele Beruhtesfa , Olani Abebe , Koran Tafesse , Tadesse Biniam , Mekonnen Getnet Abie , Almaw Gizat , Sahlu Temertu , Seyoum Bekele , Beyecha Kebede , Gumi Balako , Ameni Gobena , Ashenafi Hagos , Bakker Douwe , Kapur Vivek , Gebre Solomon
Published: March 2022

Bovine tuberculosis (bTB) is one of the top three, high-priority, livestock diseases in Ethiopia and hence, the need for evaluation of potential control strategies is critical. Here, we applied the test-and-segregate followed by cull strategy for the control of bTB in the intensive Alage dairy farm in Ethiopia. All cattle reared on this farm were repeatedly skin tested using the Comparative Cervical Tuberculin (CCT) test for a total of five times between 2015 and 2021. During the first (October 2015) and second (March 2017) rounds of testing, all reactor animals (>4 mm) were culled, while those that were deemed as inconclusive (1–4 mm) were segregated and retested. At retest, animals with CCT >2 mm were removed from the herd. In the third (December 2017) and fourth (June 2018) rounds of tuberculin testing, a more stringent approach was taken wherein all reactors per the severe mode of CCT test interpretation (>2 mm) were culled. A final herd status check was performed in May 2021. In summary, the number of CCT positives (>4 mm) in the farm dropped from 23.1% (31/134) in October 2015 to 0% in December 2017 and remained 0% until May 2021. In contrast, the number of Single Cervical Tuberculin (SCT) test positives (≥4 mm) increased from 1.8 to 9.5% (from 2017 to 2021), indicating that CCT test might not be sufficient to effectively clear the herd of bTB. However, a more stringent approach would result in a drastic increase in the number of false positives. The total cost of the bTB control effort in this farm holding 134–200 cattle at any given time was conservatively estimated to be ~US$48,000. This, together with the need for culling an unacceptably high number of animals based on skin test status, makes the test-and-cull strategy impractical for nationwide implementation in Ethiopia and other low- and middle-income countries (LMICs) where the infection is endemic. Hence, there is an increased emphasis on the need to explore alternate, affordable measures such as vaccination alongside accurate diagnostics to help control bTB in endemic settings.
A Concept for a Mars Boundary Layer Sounding Balloon: Science Case, Technical Concept and Deployment Risk Analysis
Authors: Witte Lars , Arnold Gabriele , Bertram Jan , Grott Matthias , Krämer Caroline , Lorek Andreas , Wippermann Torben
Published: March 2022

The Mars Exploration Program Analysis Group has identified measurements of the state and the variability of the Martian atmosphere as high priority investigations for the upcoming years. Balloon-borne instruments could bridge the gap in both temporal and spatial resolution in mesoscale distances between local, stationary landers and global orbiter observations. The idea to use a balloon system for such a purpose is not new in essence and has been proposed already in past decades. While those concepts considered an aerial deployment during entry and descent, the concept outlined in this study revisits a launch off the payload deck of a lander from the Martian surface. This deployment option profits today mainly from the technological advance in micro-electronics and sensor miniaturization, which enables the design of a balloon-probe significantly smaller than earlier proposed systems. This paper presents the feasibility assessment for this instrument and gives further details on the scientific and operational concept, a strawman sensor suite, its system components and the associated size and budget estimates. It is complemented by the analysis scheme proposed to assess, manage and mitigate the deployment risk involved in automatically launching such a balloon-system off a planetary surface.
Event-Triggered Attitude-Tracking Control for a Cableless Non-Contact Close-Proximity Formation Satellite
Authors: Qi Jirong , Liao He , Xu Yufei , Zhu Zhu , You Chaolan
Published: March 2022

The cablelessness of non-contact close-proximity formation satellites can fundamentally avoid the influence of non-contact interface coupling effects and can further enhance the attitude pointing accuracy and stability of the payload module (PM). However, it also brings the problem of limited on-board resources and system latency. In this paper, an event-triggered attitude tracking controller of the support module (SM) that avoids the Zeno phenomenon was proposed. The update time of the control signal was determined by the event-triggering mechanism based on intermediate variables, thus, reducing the communication burden and actuator asynchrony between the two modules. The feasibility and effectiveness of the proposed approach was demonstrated by numerical simulations.
International Passenger Traffic at the Hellenic Airports: Impact of the COVID-19 Pandemic and Mid-Term Forecasting
Authors: Kitsou Stavroula P. , Koutsoukis Nikitas Spiros , Chountalas Panos , Rachaniotis Nikolaos P.
Published: March 2022

The aviation industry in the COVID-19 pandemic era (caused by the SARS-CoV-2 pathogen) is practically starting from scratch as significant losses have been recorded since the outbreak in December 2019. This paper focuses on the study of the available historical operational data (provisional and final) of international passenger traffic to and from the Hellenic airports. The data originated from the official state statistics agency (Hellenic Civil Aviation Authority, HCAA). A forecasting model was developed to provide an estimation of the international passenger traffic at the Hellenic airports from August to December 2021. Through this analysis, the severe impact of two lockdown events and how they will affect the expected recovery rate of air travel is demonstrated. From this perspective, some first conclusions can be drawn about the expected recovery of international air travel to the growth rates of the 2019 pre-pandemic levels.
Germination and the Biochemical Response of Pumpkin Seeds to Different Concentrations of Humic Acid under Cadmium Stress
Authors: Asadi Aghbolaghi Masoumeh , Sedghi Mohammad , Sharifi Raouf Seyed , Dedicova Beata
Published: March 2022

The poisoning of heavy metals and their accumulation in food chains are major environmental and health risks. There have been several reports that determined that pumpkins tend to collect small amounts of nitrate or heavy metals. Therefore, the aim of the present study is to investigate the effect of organic matter (humic acid) on the germination and activity of antioxidant enzymes, glycosylate cycle enzymes, and utilization of lipid and protein reserves of pumpkin seeds under cadmium stress conditions. An experiment was conducted to quantify the germination response and biochemical change of pumpkin seeds to the use of humic acid under cadmium stress conditions. The treatments were cadmium at three levels (0 (control), 100, and 200 mg.L−1) and humic acid at five levels (0 (control), 100, 200, 300, and 400 mg.L−1). Linear and sigmoidal models were used to investigate the trend of trait changes. The results show that changes in the germination percentage and seed vigor were affected by applying humic acid and cadmium stress. The highest germination percentage for pumpkins was observed without stress and cadmium stress at a concentration of 200 mg.L−1. The results of quantification for the germination and seed vigor also showed that the model of germination changes by the use of humic acid was sigmoidal in non-stress and cadmium stress conditions of 100 mg.L−1, but it was linear for seed vigor in the stress conditions of 200 mg.L−1. The activity of superoxide dismutase, catalase, peroxidase, isocitrate lyase, and malate synthase was also affected by the simultaneous use of humic acid and cadmium stress, and the trend of their changes was linear.
The Influence of Nesfatin-1 on Bone Metabolism Markers Concentration, Densitometric, Tomographic and Mechanical Parameters of Skeletal System of Rats in the Conditions of Established Osteopenia
Authors: Tymicki Grzegorz , Puzio Iwona , Pawłowska-Olszewska Marta , Bieńko Marek , Radzki Radosław Piotr
Published: March 2022

Our study aimed to evaluate the impact of nesfatin-1 administration on bone metabolism and properties in established osteopenia in ovariectomized female rats. In total, 21 female Wistar rats were assigned to two groups: sham-operated (SHAM, n = 7) and ovariectomized (OVA, n = 14). After 12 weeks of osteopenia induction in the OVA females, the animals were given i.p. physiological saline (OVA, n = 7) or 2 µg/kg body weight of nesfatin-1(NES, n = 7) for the next 8 weeks. The SHAM animals received physiological saline at the same time. Final body weight, total bone mineral density and content of the skeleton were estimated. Then, isolated femora and tibias were subjected to densitometric, tomographic, and mechanical tests. Bone metabolism markers, i.e., osteocalcin, bone specific alkaline phosphatase (bALP), and crosslinked N-terminal telopeptide of type I collagen (NTx) were determined in serum using an ELISA kit. Ovariectomy led to negative changes in bone metabolism associated with increased resorption, thus diminishing the densitometric, tomographic, and mechanical parameters. In turn, the administration of nesfatin-1 led to an increase in the value of the majority of the tested parameters of bones. The lowest bALP concentration and the highest NTx concentration were found in the OVA females. The bALP concentration was significantly higher after nesfatin-1 administration in comparison to the OVA rats. In conclusion, the results indicate that nesfatin-1 treatment limits bone loss, preserves bone architecture, and increases bone strength in condition of established osteopenia.
African Swine Fever—How to Unravel Fake News in Veterinary Medicine
Authors: Trotta Adriana , Marinaro Mariarosaria , Cavalli Alessandra , Cordisco Marco , Piperis Angela , Buonavoglia Canio , Corrente Marialaura
Published: March 2022

In recent years, fake scientific news has spread much faster through the Internet and social media within the so-called “infodemic”. African Swine Fever (ASF) is a perfect case study to prove how fake news can undermine the public health response, even in the veterinary field. ASF is a highly contagious infective disease affecting exclusively domestic and wild pigs such as wild boars. ASF can cause social damage and economic losses both directly (due to the high mortality rate) and indirectly (due to international sanctions). Although ASF is not a threat to human health, since 2018 newspapers have often reported false or misleading news, ranging from misinterpreted findings/data to fake or alarmistic news. In some cases, fake news was spread, such as the use of snipers at the border of nations to kill wild boars, or those reports concerning possible risks to human health. In order to provide real and fact-based news on epidemics, some organizations have created easy-to-read infographic and iconographic materials, available on their websites, to help the readers identifying the fake news. Indeed, it is crucial that governments and scientific organizations work against fear and anxiety, using simple and clear communication.
Origin, Migration, and Reproduction of Indigenous Domestic Animals with Special Reference to Their Sperm Quality
Authors: van der Horst Gerhard , Maree Liana
Published: March 2022

Indigenous domestic animals such as cattle, sheep, goats, pigs, and chickens have a natural resistance to endo- and ecto-parasites and are tolerant in terms of harsh environmental conditions. These species orginated from the Fertile Cresent between 12,000 and 10,000 BP before migrating into surrounding continents. In view of limited information on the reproductive status of indigenous breeds, it is important to examine their semen characteristics in order to select males to improve livestock production. We have largely relied on existing literature but also our published and ongoing research on sperm quality assessment of several indigenous breeds. The sperm quality of these breeds is similar to current commercial breeds and has been quantified using cutting-edge methods. In this context, we have presented sperm functional tests which provide a better estimate of semen quality than just a standard semen analysis. Initial results suggest that the indigenous breeds have a high sperm quality and sperm functionality similar to currently farmed exotic or crossbreeds. In the long-term, the importance of preserving the favorable traits of these breeds is a priority in view of crossbreeding with existing good meat and milk producers.
Associations between Animal Welfare Indicators and Animal-Related Factors of Slaughter Cattle in Austria
Authors: Burgstaller Johann , Wittek Thomas , Sudhaus-Jörn Nadine , Conrady Beate
Published: March 2022

Three cattle welfare indicators (lameness, dirtiness, and abomasal disorders) were evaluated in 412 slaughter cattle in a cross-sectional study in Austria. The aims of this study were (1) to evaluate the prevalence of lameness, dirtiness of slaughter cattle, and abomasal disorders; and (2) to determine the association between these welfare indicators and animal-related factors (e.g., housing type, carcass weight, transportation and waiting duration of the animals). The lameness prevalence was 0.73%, the abomasal disorders prevalence was 52.43%, and 88.59% of all cattle were contaminated. The latter result indicates that the cattle were kept in a dirty environment. The occurrence of abomasal disorders was associated with cattle housing systems (p ≤ 0.00) and slaughter weight (p = 0.03). The odds for abomasal disorders were 28.0 times higher for cattle housed on slatted flooring compared to cattle kept in a tethered system. The chance for occurrence of abomasal disorders was 3.6 times higher for cattle with a low carcass weight compared to cattle with a high carcass weight. Furthermore, significant associations were found between dirtiness (also referred to as cleanliness or contamination) and husbandry system, sex, and breed. Cattle housed in deep litter boxes had 40.8 times higher odds of being contaminated compared to cattle in a tethered housing system. Cows (odds: 32.9) and heifers (odds: 4.4) had higher odds of being contaminated with feces compared to bulls, whereby female calves (odds: 0.09) and male calves (odds: 0.02) had significantly lower odds of being contaminated. Furthermore, the breeds Brown Swiss (odds: 0.26) and Holstein-Friesian (odds: 0.14) had a significantly lower chance of being contaminated compared to Simmental cattle. Other collected factors, such as production system, transportation duration, life days of the cattle, average daily weight gain, carcass classification, and fat coverage, showed no association with the collected welfare indicators. The study presented here indicates that welfare indicators evaluated for slaughter cattle are suitable to assess cattle welfare, and improvements in husbandry may positively impact both the abomasal physiology and cleanliness of cattle.
The Arrangement of the Peripheral Olfactory System of Pleuragramma antarcticum: A Well-Exploited Small Sensor, an Aided Water Flow, and a Prominent Effort in Primary Signal Elaboration
Authors: Aicardi Stefano , Bozzo Matteo , Amaroli Andrea , Gallus Lorenzo , Risso Beatrice , Carlig Erica , Di Blasi Davide , Vacchi Marino , Ghigliotti Laura , Ferrando Sara
Published: March 2022

The olfactory system is constituted in a consistent way across vertebrates. Nasal structures allow water/air to enter an olfactory cavity, conveying the odorants to a sensory surface. There, the olfactory neurons form, with their axons, a sensory nerve projecting to the telencephalic zone—named the olfactory bulb. This organization comes with many different arrangements, whose meaning is still a matter of debate. A morphological description of the olfactory system of many teleost species is present in the literature; nevertheless, morphological investigations rarely provide a quantitative approach that would help to provide a deeper understanding of the structures where sensory and elaborating events happen. In this study, the peripheral olfactory system of the Antarctic silverfish, which is a keystone species in coastal Antarctica ecosystems, has also been described, employing some quantitative methods. The olfactory chamber of this species is connected to accessory nasal sacs, which probably aid water movements in the chamber; thus, the head of the Antarctic silverfish is specialized to assure that the olfactory organ keeps in contact with a large volume of water—even when the fish is not actively swimming. Each olfactory organ, shaped like an asymmetric rosette, has, in adult fish, a sensory surface area of about 25 mm2, while each olfactory bulb contains about 100,000 neurons. The sensory surface area and the number of neurons in the primary olfactory brain region show that this fish invests energy in the detection and elaboration of olfactory signals and allow comparisons among different species. The mouse, for example—which is considered a macrosmatic vertebrate—has a sensory surface area of the same order of magnitude as that of the Antarctic silverfish, but ten times more neurons in the olfactory bulb. Catsharks, on the other hand, have a sensory surface area that is two orders of magnitude higher than that of the Antarctic silverfish, while the number of neurons has the same order of magnitude. The Antarctic silverfish is therefore likely to rely considerably on olfaction.
The Characteristics and Distribution of α2D-, α2B- and α2C-Adrenoceptor Subtypes in Goats
Authors: Xu Ming , Zhang Qiulin , Wang Qi , Pan Di , Ding Mingxing , Ding Yi
Published: March 2022

α2-Adrenegic receptors (α2Rs) are important presynaptic modulators of central noradrenergic function (auto receptors) and postsynaptic mediators of many of the widespread effects of catecholamines and related drugs. Studies have shown that ruminants (such as goats and cattle) express special α2DR subtypes in addition to α2BR and α2CR. Real-time quantitative PCR and Western blotting were used to investigate the distribution and density of α2R in different nuclei of the goat central nervous system, selected regions of the spinal cord (L4-L6), and in various peripheral tissues. α2-AR subtype-specific antibodies were injected intrathecally and intracerebroventricularly into the tested goats to block the corresponding subtype of receptors. Pain threshold and physiological parameters were evaluated to explore the functional characteristics of α2BR, α2CR and α2DR in goats. Our results suggest that the expression of the mRNAs and proteins of all three α2R subtypes are widely but unevenly distributed in the goat CNS and peripheral tissues. Furthermore, α2DR plays a more important role in α2R-mediated analgesia in goats than α2BR and α2CR, whereas α2CR activation exerts a greater effect on body temperature than α2BR and α2DR.
Learning Ability and Hippocampal Transcriptome Responses to Early and Later Life Environmental Complexities in Dual-Purpose Chicks
Authors: Yan Chao , Liu Wen , Xiao Jinlong , Xiang Hai , Wang Jikun , Zhang Hui , Liu Jian , Chen Siyu , Zhao Xingbo
Published: March 2022

In this study, we hypothesized that complex early-life environments enhance the learning ability and the hippocampal plasticity when the individual is faced with future life challenges. Chicks were divided into a barren environment group (BG), a litter materials group (LG), and a perches and litter materials group (PLG) until 31 days of age, and then their learning abilities were tested following further rearing in barren environments for 22 days. In response to the future life challenge, the learning ability showed no differences among the three groups. In the hippocampal KEGG pathways, the LG chicks showed the downregulation of neural-related genes neuronal growth regulator 1 (NEGR1) and neurexins (NRXN1) in the cell adhesion molecules pathway compared to the BG (p < 0.05). Immune-related genes TLR2 in Malaria and Legionellosis and IL-18 and IL18R1 in the TNF signaling pathway were upregulated in the LG compared to in the BG (p < 0.05). Compared to the BG, the PLG displayed upregulated TLR2A in Malaria (p < 0.05). The PLG showed upregulated neural-related gene, i.e., neuronal acetylcholine receptor subunit alpha-7-like (CHRNA8) in the nicotine addiction pathway and secretagogin (SCGN) gene expression, as compared to the LG (p < 0.05). In conclusion, early-life environmental complexities had limited effects on the learning ability in response to a future life challenge. Early-life perches and litter materials can improve neural- and immune-related gene expression and functional pathways in the hippocampus of chicks.
Metabolic Basis and Clinical Evidence for Skin Lightening Effects of Thiol Compounds
Authors: Boo Yong Chool
Published: March 2022

Melanin pigment is a major factor in determining the color of the skin, and its abnormal increase or decrease can cause serious pigmentation disorders. The melanin pigment of the skin is divided into light pheomelanin and dark eumelanin, and a big difference between them is whether they contain sulfur. Melanin synthesis starts from a common reaction in which tyrosine or dihydroxyphenylalanine (DOPA) is oxidized by tyrosinase (TYR) to produce dopaquinone (DQ). DQ is spontaneously converted to leukodopachrome and then oxidized to dopachrome, which enters the eumelanin synthesis pathway. When DQ reacts with cysteine, cysteinyl dopa is generated, which is oxidized to cysteinyl DQ and enters the pheomelanin synthesis pathway. Therefore, thiol compounds can influence the relative synthesis of eumelanin and pheomelanin. In addition, thiol compounds can inhibit enzymatic activity by binding to copper ions at the active site of TYR, and act as an antioxidant scavenging reactive oxygen species and free radicals or as a modulator of redox balance, thereby inhibiting overall melanin synthesis. This review will cover the metabolic aspects of thiol compounds, the role of thiol compounds in melanin synthesis, comparison of the antimelanogenic effects of various thiol compounds, and clinical trials on the skin lightening efficacy of thiol compounds. We hope that this review will help identify the advantages and disadvantages of various thiol compounds as modulators of skin pigmentation and contribute to the development of safer and more effective strategies for the treatment of pigmentation disorders.
Bioactive Peptide Fractions from Collagen Hydrolysate of Common Carp Fish Byproduct: Antioxidant and Functional Properties
Authors: González-Serrano Diego J. , Hadidi Milad , Varcheh Matin , Jelyani Aniseh Zarei , Moreno Andres , Lorenzo Jose M.
Published: March 2022

Collagen isolated from byproducts of common carp was hydrolyzed with alcalase enzyme to obtain peptide fractions. The resulting >30 kDa (PF1), 10–30 kDa (PF2), 3–10 kDa (PF3) and <1 kDa (PF4) fractions were studied for their antioxidant and functional properties. All peptide fractions illustrated antioxidant activity at different concentrations (1, 5, and 10 mg/mL). Although PF4 indicated the highest DPPH radical-scavenging activity (87%) at a concentration of 1 mg/mL, the highest reducing power (0.34) and hydroxyl radical scavenging activity (95.4%) were also observed in PF4 at a concentration of 10 mg/mL. The solubility of the peptide fractions was influenced by pH. The lowest solubility of the peptide fractions was observed at pH 4. The highest emulsifying activity index (EAI) was observed for PF4 (121.1 m2/g), followed by PF3 (99.6 m2/g), PF2 (89.5 m2/g) and PF1 (78.2 m2/g). In contrast to what has been found in the case of EAI, the emulsion stability of the peptide fractions decreased at lower molecular weight, which ranged from 24.4 to 31.6 min. Furthermore, it was revealed that PF1 had the highest foam capacity (87.4%) and foam stability (28.4 min), followed by PF2 and PF3. Overall, the findings suggest that peptide fractions isolated from byproducts of common carp are a promising source of natural antioxidants for application in functional food and pharmaceutical products.
Apoptosis-Inducing Factor Deficiency Induces Tissue-Specific Alterations in Autophagy: Insights from a Preclinical Model of Mitochondrial Disease and Exercise Training Effects
Authors: Laine-Menéndez Sara , Fernández-de la Torre Miguel , Fiuza-Luces Carmen , Delmiro Aitor , Arenas Joaquín , Martín Miguel Ángel , Boya Patricia , Lucia Alejandro , Morán María
Published: March 2022

We analyzed the effects of apoptosis-inducing factor (AIF) deficiency, as well as those of an exercise training intervention on autophagy across tissues (heart, skeletal muscle, cerebellum and brain), that are primarily affected by mitochondrial diseases, using a preclinical model of these conditions, the Harlequin (Hq) mouse. Autophagy markers were analyzed in: (i) 2, 3 and 6 month-old male wild-type (WT) and Hq mice, and (ii) WT and Hq male mice that were allocated to an exercise training or sedentary group. The exercise training started upon onset of the first symptoms of ataxia in Hq mice and lasted for 8 weeks. Higher content of autophagy markers and free amino acids, and lower levels of sarcomeric proteins were found in the skeletal muscle and heart of Hq mice, suggesting increased protein catabolism. Leupeptin-treatment demonstrated normal autophagic flux in the Hq heart and the absence of mitophagy. In the cerebellum and brain, a lower abundance of Beclin 1 and ATG16L was detected, whereas higher levels of the autophagy substrate p62 and LAMP1 levels were observed in the cerebellum. The exercise intervention did not counteract the autophagy alterations found in any of the analyzed tissues. In conclusion, AIF deficiency induces tissue-specific alteration of autophagy in the Hq mouse, with accumulation of autophagy markers and free amino acids in the heart and skeletal muscle, but lower levels of autophagy-related proteins in the cerebellum and brain. Exercise intervention, at least if starting when muscle atrophy and neurological symptoms are already present, is not sufficient to mitigate autophagy perturbations.
Social Perception of Artificial Intelligence and Digitization of Cultural Heritage: Russian Context
Authors: Leshkevich Tatiana , Motozhanets Anna
Published: March 2022

The article considers the social perception of artificial intelligence (AI) as an essential factor having an impact on the digitization, transfer, and popularization of cultural heritage. The article aims at the theoretical comprehension of the digital ontology and implementation of AI in the context of Russian realia. The research relies on comprehensive analysis based on statistical data, using descriptive and comparative methods. The interdisciplinary approach adopted includes reflexive analysis of both positive and negative consequences of the digitalization process. The article examines the peculiarities of the “digital ontology”, deterritorization effect, new actors in the digital process, the effect of digital trust, and opposite views of AI-enthusiasts and AI-alarmists. The article describes objective and subjective reasons for the negative perception of digital artifacts, and states the need to consider the influence of key figures in digital ontology: influencers, stakeholders, and data scientists. The ambivalence of public perception of both artificial intelligence and digital cultural heritage is stated. The research reveals digitization frontiers, which involve three factors: the axiological factor, indicating the need for consistency between a human values system and AI development programs; the subject vector, emphasizing the role of a new type of digital heritage bearers and digital influence actors; ethical factor associated with the need to transform relations with information technologies in order to subordinate them to human reflection and comprehension. The authors conclude that cultural heritage digitization should be aimed at generating a human-centered future.
Sepiolite-Based Anisotropic Nanoparticles: A New Player in the Rubber Reinforcement Technology for Tire Application
Authors: Tadiello Luciano , Guerra Silvia , Giannini Luca
Published: March 2022

Elastomer reinforcement with nanofillers has been a main rubber technology topic since the discovery of rubber vulcanization. Starting from carbon black, many researchers studied the correlations between the reinforcement of rubber and the colloidal properties of the reinforcing filler. The advent of silica allowed the experimentation of a playground of chemistries at the filler-rubber interface. In the increasing complexity of nowadays car manufacturer requests, reinforcing fillers play a pivotal role in determining the set of properties, which make a specific compound suitable for its applications. This effort of continuous improvement of rubber compound properties is pushing the tire industry towards novel solutions, and the silica/CB filler reinforcing technology will likely soon accept at least a third reinforcing filler as a major constituent of rubber compounds. While all major tire manufacturers build continuous knowledge on candidates such as carbon allotropes and 2-D nanoclays, Pirelli is paving the way for Sepiolite-derived nanofillers. Being Sepiolite naturally sourced, safe, and chemically playful, this unique 1-D phyllosilicate bears the promise of changing the game of elastomer nano-reinforcement, with the optional characteristic of giving mechanical anisotropy to the rubber compound. In this review, the historical progress on rubber reinforcement with sepiolite will be summarized, with an example of application in a commercially available Pirelli product, progenitor of the Smart-Net Silica® technology.
Operational Modal Analysis of an Axial Compressor Rotor and Casing System for the Online Identification of a Digital Twin
Authors: Amer Mona , Wallaschek Joerg , Seume Joerg R.
Published: March 2022

Modal parameter identification can be a valuable tool in mechanical engineering to predict vibrational behaviour and avoid machine damage during operation. Operational modal analysis is an output-only identification tool motivated by the structural identification of civil engineering structures, which are excited by ambient conditions. This technique is increasingly applied in mechanical engineering in order to characterise the system behaviour during operation as modal parameters can vary under operating conditions. The following study investigates the application of operational modal analysis on an axial compressor under operating conditions. Since the modal parameters of the system change depending on the life history and during the operation of the system, a corresponding data analysis might allow us to identify the present status of the system. Eigenfrequencies and eigenvectors are studied for the use of structural health monitoring approaches. According to the analysis, eigenfrequencies represent robust parameters for the studied purpose. Eigenvectors are sensitive to damages but need further investigation, especially for rotating machinery. This study will help the user to set up a virtual model, which describes the system behaviour for different boundary conditions. This in turn, will provide an accurate prediction of the vibrational behaviour in order to assure a safe operation.
Dynamic Variability of Wind Erosion Climatic Erosivity and Their Relationships with Large-Scale Atmospheric Circulation in Xinjiang, China
Authors: Wang Yaqin , Yang Haimei , Fan Wenbo , Qiao Changlu , Sun Kai
Published: March 2022

Xinjiang has a serious wind erosion problem due to its fragile ecological condition and sensitivity to climate change. Wind erosion climatic erosivity is a measure of climatic factors influencing wind erosion; evaluating its spatiotemporal variations and relationship with the large-scale circulation pattern can contribute to the understanding of the climate change effect on wind erosion risk. Thus, this study quantified the wind erosion climatic erosivity and examined the connections between climatic erosivity and climate indices using trend analysis, geo-statistical analysis, and cross-wavelet analysis based on the observed daily meteorological data from 64 weather stations in Xinjiang, China during 1969–2019 (50 years). The results indicated that the climatic erosivity showed a significant downward trend at seasonal and annual scales over the past 50 years. Strong seasonality in the C-factor was found, with its highest values in the spring and summer and its lowest values in the winter. The average climatic erosivity was weaker during El Niño events than during La Niña events. The impact of El Niño events on climatic erosivity in Xinjiang continued from the beginning of the event to two months after the end of the events. The La Niña events had a lag effect on the climatic erosivity in Xinjiang, with a lag period of 4 months. From a statistical perspective, the El Niño-Southern Oscillation (ENSO), North Atlantic Oscillation (NAO), and Arctic Oscillation (AO) indices showed relationships to the climatic erosivity in Xinjiang in terms of their correlation and periodicity. The relationships between the climatic erosivity and ENSO were not clearly positive or negative, with many correlations advanced or delayed in phase. The NAO and AO indices showed a consistent in-phase relationship with climatic erosivity on significant bands, whereas the profound mechanisms involved in this require further study. The results of this study provide a preliminary perspective on the effect of large-scale atmospheric circulation on wind erosion risk in arid and semi-arid regions.
Effects of Land Cover Changes on Compound Extremes over West Africa Using the Regional Climate Model RegCM4
Authors: Camara Moctar , Diba Ibrahima , Diedhiou Arona
Published: March 2022

This study aims to characterize the impacts of the Sahel–Sahara interface reforestation on compound extremes in the Sahel region during the West African monsoon season (June–July–August–September, JJAS). For this purpose, we performed a simulation with the standard version of the RegCM4 model, and another simulation with the altered version of the same model, taking into account the incorporated forest. Results show that reforestation may strongly influence the frequency of individual extreme events (dry and warm days) by decreasing them over and off the reforested zone. The reduction in these extreme dry and warm days may be due partly to the strengthening of the atmospheric moisture content over most parts of the West African domain and the weakening of the sensible heat flux south of 16° N. The analysis also shows an increase in extreme wet days over and off the reforested zone, which could be associated partly with the strengthening of evapotranspiration over most parts of the West African domain, including the reforested area. The analysis of compound extremes shows a strong occurrence of the compound dry/warm mode over the northern Sahel for both runs, probably due to the weak precipitation recorded in this zone. Both experiments also simulated a strong compound wet/warm mode occurrence over the Sahel due to a high rainfall occurrence over this region. When comparing both runs, the impact of the reforestation was to decrease (increase) the compound extreme dry/warm (wet/warm) mode over the reforested zone. The dry/warm mode decrease is consistent with that of individual extreme dry and warm days, while the compound wet/warm mode increase may be driven by that of the extreme wet days. Finally, when considering the seasonal cycle, the dry/warm mode exhibits a more substantial decrease in the beginning (June–July, JJ) than during the peak of the West African summer monsoon season (August–September, AS). Moreover, reforestation similarly affects the compound wet/warm mode in JJ and AS by increasing it in the reforested region and decreasing it over the Southern Sahel (south of 15° N). This work suggests that reforestation may be a good solution for West African policymakers to mitigate climate change over the region and to develop better strategies for water resource management.
Exploring Meteorological Conditions and Human Health Impacts during Two Dust Storm Events in Northern Cape Province, South Africa: Findings and Lessons Learnt
Authors: Nkosi Vusumuzi , Mathee Angela , Blesic Suzana , Kapwata Thandi , Kunene Zamantimande , du Preez David Jean , Garland Rebecca , Wright Caradee Yael
Published: March 2022

Dust storms are meteorological hazards associated with several adverse health impacts including eye irritations, respiratory and cardiovascular disorders, and vehicular road accidents due to poor visibility. This study investigated relations between admissions from a large, public hospital that serves people living in Northern Cape and Free State provinces, South Africa during 2011 to 2017, and meteorological variables (temperature and air quality) during two dust storms, one in October 2014 (spring) and the second in January 2016 (summer), identified from the media as no repository of such events exists for South Africa. Distributed nonlinear lag analysis and wavelet transform analysis were applied to explore the relationships between hospital admissions for respiratory and cardiovascular diseases, eye irritation, and motor vehicle accidents; maximum temperature, and two air quality ‘proxy measures,’ aerosol optical depth and Ångström exponent, were used as ground-based air quality data were unavailable. Eye irritation was the most common dust-related hospital admission after both dust storm events. No statistically significant changes in admissions of interest occurred at the time of the two dust storm events, using either of the statistical methods. Several lessons were learnt. For this type of study, ground-based air quality and local wind data are required; alternative statistical methods of analysis should be considered; and a central dust storm repository would help analyze more than two events. Future studies in South Africa are needed to develop a baseline for comparison of future dust storm events and their impacts on human health.
Analysis of Wildfires in the Mid and High Latitudes Using a Multi-Dataset Approach: A Case Study in California and Krasnoyarsk Krai
Authors: Shikwambana Lerato , Habarulema John Bosco
Published: March 2022

In this study, we investigate the emissions from wildfires in the mid latitude (California) and high latitude (Krasnoyarsk Krai) during the periods of 16–17 August 2020 and 28 July 2019, respectively. Wildfires are unique in themselves as they are driven by various factors such as fuel type, topology, and meteorology. In this study, we analyze whether there are any major variations in the emissions and transport of pollutants between two large wildfire cases in the mid latitude of California and high latitude of Krasnoyarsk Krai. The study is important to understand and characterize the emission regime from biomass burning of different land covers using a mutli-dataset approach. We analyze whether there are any major variations in the emissions and transport of pollutants from these wildfires. For example, the aerosol extinction coefficient profile showed smoke detected at the highest altitude of 9 km in Krasnoyarsk Krai, whereas in California the highest altitude was observed at approximately 6 km. Moreover, large values of black carbon (BC) concentration were observed in Krasnoyarsk Krai approximately 7 µg/m3 compared to the 0.44 µg/m3 observed in California. Areas with an immense dense vegetation are prone to large emissions. The results from this case study suggest that high latitude wildfires emit more pollutants than mid latitude wildfires. However, more studies in the future will be conducted to conclude this observation and finding with certainty.
Comparison of Object Detection in Head-Mounted and Desktop Displays for Congruent and Incongruent Environments
Authors: Reinhard René , Telatar Erinchan , Humayoun Shah Rukh
Published: March 2022

Virtual reality technologies, including head-mounted displays (HMD), can provide benefits to psychological research by combining high degrees of experimental control with improved ecological validity. This is due to the strong feeling of being in the displayed environment (presence) experienced by VR users. As of yet, it is not fully explored how using HMDs impacts basic perceptual tasks, such as object perception. In traditional display setups, the congruency between background environment and object category has been shown to impact response times in object perception tasks. In this study, we investigated whether this well-established effect is comparable when using desktop and HMD devices. In the study, 21 participants used both desktop and HMD setups to perform an object identification task and, subsequently, their subjective presence while experiencing two-distinct virtual environments (a beach and a home environment) was evaluated. Participants were quicker to identify objects in the HMD condition, independent of object-environment congruency, while congruency effects were not impacted. Furthermore, participants reported significantly higher presence in the HMD condition.
Effectiveness and Safety of Treatment with Neodymium:YAG Laser 1064 nm in Patients with Classic and Epidemic Kaposi Sarcoma
Authors: Silvestri Martina , Latini Alessandra , Lesnoni La Parola Ilaria , Messina Claudia , Nisticò Steven Paul , Cameli Norma
Published: March 2022

Kaposi’s sarcoma (KS) is a vascular neoplasm Herpes Virus 8 (HHV8), which can affect the skin, mucous membranes and viscera. There is currently no standard treatment for KS; this study evaluated the efficacy and safety of Neodymium:YAG (Nd:YAG) laser 1064 nm treatment in patients with classic and HIV-associated KS. 15 patients with classic KS (group A) and 15 with epidemic KS (group B), with exclusively cutaneous localization, were treated with Nd:YAG laser 1064 nm. Four treatment sessions were performed at 4 weeks intervals. 24/30 (80%) of treated patients underwent clinical improvement. Better results have been obtained in HIV-positive patients, especially in terms of reduced lesion size and the flattening of elevated lesions. The 1064 nm Nd:YAG laser is effective and safe in the treatment of classic and epidemic KS, especially in patients with symptomatic, slow-progressing local disease, where other treatment options may be inappropriate.