bluuebunny/crossref_metadata_2025 huggingface dataset:
Aspirin Attenuates Hyperoxia-Induced Acute Respiratory Distress Syndrome (ARDS) by Suppressing Pulmonary Inflammation via the NF-κB Signaling Pathway
Authors: Tung Yu-Tang , Wei Chi-Hsuan , Yen Chih-Ching , Lee Po-Ying , Ware Lorraine B. , Huang Hao-En , Chen Wei , Chen Chuan-Mu
Published: January 2022

Acute respiratory distress syndrome (ARDS) is a common destructive syndrome with high morbidity and mortality rates. Currently, few effective therapeutic interventions for ARDS are available. Clinical trials have shown that the effectiveness of aspirin is inconsistent. The contribution of platelets to the inflammatory response leading to the development of ARDS is increasingly recognized. The antiplatelet agent aspirin reportedly exerts a protective effect on acid- and hyperoxia-induced lung injury in murine models. Our previous study showed that pretreatment with aspirin exerts protective effects on hyperoxia-induced lung injury in mice. However, the mechanisms and therapeutic efficacy of aspirin in the posttreatment of hyperoxia-induced acute lung injury (ALI) remain unclear. In this study, we used a homozygous NF-κB-luciferase+/+ transgenic mouse model and treated mice with low-dose (25 μg/g) or high-dose (50 μg/g) aspirin at 0, 24, and 48 h after exposure to hyperoxia (inspired oxygen fraction (FiO2) > 95%). Hyperoxia-induced lung injury significantly increased the activation of NF-κB in the lung and increased the levels of macrophages infiltrating the lung and reactive oxygen species (ROS), increased the HO-1, NF-κB, TNF-α, IL-1β, and IL-4 protein levels, and reduced the CC10, SPC, eNOS, Nrp-1, and IκBα protein levels in the lung tissue. Pulmonary edema and alveolar infiltration of neutrophils were also observed in the lung tissue of mice exposed to hyperoxia. However, in vivo imaging revealed that posttreatment with aspirin reduced luciferase expression, suggesting that aspirin might reduce NF-κB activation. Posttreatment with aspirin also reduced hyperoxia-induced increases in the numbers of lung macrophages, intracellular ROS levels, and the expression of TNF-α, IL-1β, and IL-4; it also increased CC10, SPC and Nrp-1 levels compared with hyperoxia exposure alone. Lung histopathology also indicated that the aspirin posttreatment significantly reduced neutrophil infiltration and lung edema compared with hyperoxia exposure alone. Aspirin effectively induces an anti-inflammatory response in a model of hyperoxia-induced lung injury. Thus, aspirin may have potential as a novel treatment for hyperoxia-induced ALI.
Red Yeast Rice for Hyperlipidemia: A Meta-Analysis of 15 High-Quality Randomized Controlled Trials
Authors: Li Pengfan , Wang Qi , Chen Kanjun , Zou Shihui , Shu Shi , Lu Chanchan , Wang Shiyun , Jiang Yunqin , Fan Chunxiang , Luo Yue
Published: January 2022

Background: RYR is a commonly used lipid-lowering dietary supplements in Asian and European countries, showing considerable benefits and low toxicity. This quantitative study aims to present high-quality evidence regarding the efficacy and safety of RYR in treating hyperlipidemia, in order to promote its clinical application.

Methods: PubMed, embase, and Cochrane Central Register of Controlled Trials databases were systematically searched, and high-quality randomized controlled trials comparing RYR with non-RYR interventions were included. RevMan5.3 software was used to conduct the meta-analysis.

Results: A total of 1,012 individuals participated in this study (481 in the experimental and 531 in the control group). In comparison to statins, RYR was more effective in lowering TG (MD, −19.90; 95% CI, −32.22 to −7.58; p = 0.002), comparable in lowering LDL-C and elevating HDL-C, and less effective in lowering TC (MD, 12.24; 95% CI, 2.19 to 22.29; p = 0.02). Compared with nutraceutical, RYR significantly reduced TC (MD, −17.80; 95% CI, −27.12 to −8.48; p = 0.0002) and LDL-C (MD, −14.40; 95% CI, −22.71 to −6.09; p = 0.0007), and elevated HDL-C (MD, 7.60; 95% CI, 4.33 to 10.87; p < 0.00001). Moreover, RYR effectively synergized nutraceutical to further reduce TC (MD, −31.10; 95% CI, −38.83 to −23.36; p < 0.00001), LDL-C (MD, −27.91; 95% CI, −36.58 to −19.24; p < 0.00001), and TG (MD, −26.32; 95% CI, −34.05 to −18.59; p < 0.00001). Additionally, RYR significantly reduced apoB (MD, −27.98; 95% CI, −35.51 to −20.45; p < 0.00001) and, whether alone or in combination, did not increase the risk of adverse events in patients with hyperlipidemia.

Conclusion: RYR at 200–4800 mg daily appears to be a safe and effective treatment for hyperlipidemia, effectively regulating blood lipid levels with an exceptional impact on TG. Looking forward, high-quality clinical trials with longer observation periods are required to evaluate the efficacy and safety of RYR as a long-term medication.

Systematic Review Registration: (https://www.crd.york.ac.uk/PROSPERO/), identifier (CRD4202128450).
Deleterious Effect of High-Fat Diet on Skeletal Muscle Performance Is Prevented by High-Protein Intake in Adult Rats but Not in Old Rats
Authors: Poggiogalle Eleonora , Rossignon Fanny , Carayon Aude , Capel Fréderic , Rigaudière Jean-Paul , De Saint Vincent Sarah , Le-Bacquer Olivier , Salles Jérôme , Giraudet Christophe , Patrac Véronique , Lebecque Patrice , Walrand Stéphane , Boirie Yves , Martin Vincent , Guillet Christelle
Published: January 2022

The phenotype of sarcopenic obesity is frequently associated with impaired muscle strength and performance. Ectopic lipid deposition may interfere with muscle anabolic response especially during aging. Evidence is scarce concerning the potential interplay among aging and nutrient imbalance on skeletal muscle functionality. The objective of the present study was to investigate the impact of protein intake in the context of an obesogenic diet on skeletal muscle functional properties and intramuscular lipid infiltration. Two groups of forty-two adult and thirty-seven old male Wistar rats were randomly divided into four groups: isocaloric standard diet (12% protein, 14% lipid, as ST12); isocaloric standard (high-protein) diet (25% protein, 14% lipid, ST25); hypercaloric high-fat (normal-protein) diet (12% protein, 45% lipid, HF12); and hypercaloric high-fat (high-protein) diet (25% protein, 45% lipid, HF25). The nutritional intervention lasted 10 weeks. Total body composition was measured through Echo-MRI. Lipids were extracted from tibialis anterior muscle and analyzed by gas-liquid chromatography. The functional properties of the plantarflexor muscles were evaluated in vivo on an isokinetic dynamometer. Maximal torque was assessed from the torque-frequency relationship in isometric condition and maximal power was evaluated from the torque-velocity relationship in concentric condition. In adult rats high-protein intake combined with high-fat diet determined a lower decrease in relative isometric torque, normalized to either FFM or body weight, compared with adult rats fed a high-fat normal-protein diet. High-fat diet was also detrimental to relative muscle power, as normalized to body weight, that decreased to a larger extent in adult rats fed a high-fat normal-protein diet than their counterparts fed a normal-fat, high-protein diet. The effect of high-fat diet observed in adults, with the enhanced protein intake (25%) conferring some kind of protection against the negative effects of HFD, may be linked to the reduced intramuscular fat in this group, which may have contributed to preserve, at least partly, the contractile properties. A potential role for high-protein diet in preventing ectopic lipid deposition needs to be explored in future research. Detrimental effects of high- fat diet on skeletal muscle performance are mitigated by high- protein intake in adult rats but not in old rats.
Natriuresis During an Acute Intravenous Sodium Chloride Infusion in Conscious Sprague Dawley Rats Is Mediated by a Blood Pressure-Independent α1-Adrenoceptor-Mediated Mechanism
Authors: Frame Alissa A. , Nist Kayla M. , Kim Kiyoung , Kuwabara Jill T. , Wainford Richard D.
Published: January 2022

The mechanisms that sense alterations in total body sodium content to facilitate sodium homeostasis in response to an acute sodium challenge that does not increase blood pressure have not been fully elucidated. We hypothesized that the renal sympathetic nerves are critical to mediate natriuresis via α1- or β-adrenoceptors signal transduction pathways to maintain sodium balance in the face of acute increases in total body sodium content that do not activate the pressure-natriuresis mechanism. To address this hypothesis, we used acute bilateral renal denervation (RDNX), an anteroventral third ventricle (AV3V) lesion and α1- or β-antagonism during an acute 1M NaCl sodium challenge in conscious male Sprague Dawley rats. An acute 1M NaCl infusion did not alter blood pressure and evoked profound natriuresis and sympathoinhibition. Acute bilateral RDNX attenuated the natriuretic and sympathoinhibitory responses evoked by a 1M NaCl infusion [peak natriuresis (μeq/min) sham 14.5 ± 1.3 vs. acute RDNX: 9.2 ± 1.4, p < 0.05; plasma NE (nmol/L) sham control: 44 ± 4 vs. sham 1M NaCl infusion 11 ± 2, p < 0.05; acute RDNX control: 42 ± 6 vs. acute RDNX 1M NaCl infusion 25 ± 3, p < 0.05]. In contrast, an AV3V lesion did not impact the cardiovascular, renal excretory or sympathoinhibitory responses to an acute 1M NaCl infusion. Acute i.v. α1-adrenoceptor antagonism with terazosin evoked a significant drop in baseline blood pressure and significantly attenuated the natriuretic response to a 1M NaCl load [peak natriuresis (μeq/min) saline 17.2 ± 1.4 vs. i.v. terazosin 7.8 ± 2.5, p < 0.05]. In contrast, acute β-adrenoceptor antagonism with i.v. propranolol infusion did not impact the cardiovascular or renal excretory responses to an acute 1M NaCl infusion. Critically, the natriuretic response to an acute 1M NaCl infusion was significantly blunted in rats receiving a s.c. infusion of the α1-adrenoceptor antagonist terazosin at a dose that did not lower baseline blood pressure [peak natriuresis (μeq/min) sc saline: 18 ± 1 vs. sc terazosin 7 ± 2, p < 0.05]. Additionally, a s.c. infusion of the α1-adrenoceptor antagonist terazosin further attenuated the natriuretic response to a 1M NaCl infusion in acutely RDNX animals. Collectively these data indicate a specific role of a blood pressure-independent renal sympathetic nerve-dependent α1-adrenoceptor-mediated pathway in the natriuretic and sympathoinhibitory responses evoked by acute increases in total body sodium.
A Novel Glycerol Kinase Gene OsNHO1 Regulates Resistance to Bacterial Blight and Blast Diseases in Rice
Authors: Xiao Xiaorong , Wang Rui , Khaskhali Shahneela , Gao Zhiliang , Guo Wenya , Wang Honggang , Niu Xiaolei , He Chaoze , Yu Xiaohui , Chen Yinhua
Published: January 2022

Glycerol-induced resistance to various pathogens has been reported in different plants. Glycerol kinase (GK), a vital rate-limiting enzyme that catalyzes glycerol conversion to glycerol-3-phosphate (G3P), participates in responses to both abiotic and biotic stresses. However, its physiological importance in rice defenses against pathogens remains unclear. In this research, quantification analysis revealed that GK levels were significantly induced in rice leaves infected by Xanthomonas oryzae pv. oryzae (Xoo) strain PXO99. A typical GK-encoding gene OsNHO1 was cloned in rice. The transcriptional levels of OsNHO1 were significantly induced by salicylic acid, jasmonic acid, and Xoo-PXO99. Ectopic expression of OsNHO1 partially rescued the resistance to P. s. pv. phaseolicola in the Arabidopsis nho1 mutant. In the overexpressing transgenic rice lines (OsNHO1-OE), the content of GK and the transcriptional level of OsNHO1 were increased and the resistance to bacterial blight and blast was improved, while reduced OsNHO1 expression impaired the resistance in OsNHO1-RNAi lines. The wax contents and expression of the wax synthesis regulatory genes were significantly increased in the overexpression lines but decreased in the OsNHO1-RNAi lines. We then confirmed the interaction partner of OsNHO1 using yeast two-hybrid and bimolecular fluorescence complementation assays. The transcription of the interaction partner-encoding genes OsSRC2 and OsPRs in OsNHO1-RNAi lines was downregulated but upregulated in OsNHO1-OE lines. Thus, we concluded that OsNHO1 provided disease resistance by affecting the wax content and modulating the transcription levels of PR genes.
The Grape Gene Reference Catalogue as a Standard Resource for Gene Selection and Genetic Improvement
Authors: Navarro-Payá David , Santiago Antonio , Orduña Luis , Zhang Chen , Amato Alessandra , D’Inca Erica , Fattorini Chiara , Pezzotti Mario , Tornielli Giovanni Battista , Zenoni Sara , Rustenholz Camille , Matus José Tomás
Published: January 2022

Effective crop improvement, whether through selective breeding or biotech strategies, is largely dependent on the cumulative knowledge of a species’ pangenome and its containing genes. Acquiring this knowledge is specially challenging in grapevine, one of the oldest fruit crops grown worldwide, which is known to have more than 30,000 genes. Well-established research communities studying model organisms have created and maintained, through public and private funds, a diverse range of online tools and databases serving as repositories of genomes and gene function data. The lack of such resources for the non-model, but economically important, Vitis vinifera species has driven the need for a standardised collection of genes within the grapevine community. In an effort led by the Integrape COST Action CA17111, we have recently developed the first grape gene reference catalogue, where genes are ascribed to functional data, including their accession identifiers from different genome-annotation versions (https://integrape.eu/resources/genes-genomes/). We present and discuss this gene repository together with a validation-level scheme based on varied supporting evidence found in current literature. The catalogue structure and online submission form provided permits community curation. Finally, we present the Gene Cards tool, developed within the Vitis Visualization (VitViz) platform, to visualize the data collected in the catalogue and link gene function with tissue-specific expression derived from public transcriptomic data. This perspective article aims to present these resources to the community as well as highlight their potential use, in particular for plant-breeding applications.
A Reasonable Officer: Examining the Relationships Among Stress, Training, and Performance in a Highly Realistic Lethal Force Scenario
Authors: Baldwin Simon , Bennell Craig , Blaskovits Brittany , Brown Andrew , Jenkins Bryce , Lawrence Chris , McGale Heather , Semple Tori , Andersen Judith P.
Published: January 2022

Under conditions of physiological stress, officers are sometimes required to make split-second life-or-death decisions, where deficits in performance can have tragic outcomes, including serious injury or death and strained police–community relations. The current study assessed the performance of 122 active-duty police officers during a realistic lethal force scenario to examine whether performance was affected by the officer’s level of operational skills training, years of police service, and stress reactivity. Results demonstrated that the scenario produced elevated heart rates (i.e., 150 beats per minute), as well as perceptual and cognitive distortions, such as tunnel vision, commensurate with those observed in naturalistic use of force encounters. The average performance rating from the scenario was 59%, with 27% of participants making at least one lethal force error. Elevated stress reactivity was a predictor of poorer performance and increased lethal force errors. Level of training and years of police service had differential and complex effects on both performance and lethal force errors. Our results illustrate the need to critically reflect on police training practices and continue to make evidence-based improvements to training. The findings also highlight that while training may significantly improve outcomes, flawless performance is likely not probable, given the limits of human performance under stress. Implications for the objective reasonableness standard, which is used to assess the appropriateness of force in courts of law, are discussed.
Environmental Problems: An Analysis of Students’ Perceptions Towards Selective Waste Collection
Authors: Gherheş Vasile , Fărcaşiu Marcela Alina , Para Iulia
Published: January 2022

The reduction, reuse, collection and recovery of recyclable materials are sustainable behaviors and people’s awareness of them plays an important role in implementing strategies and policies in this field. The quantitative analysis performed on a group of 816 students of Politehnica University of Timisoara, aimed at finding answers to important environmental concerns and observing the students’ behaviors of reuse and selective collection of the waste resulted from plastic containers, paper, aluminum, batteries, iron packaging waste, electronic equipment, used cooking oil and printer toner. The research has shown that ‘increased amounts of waste’ (63.5%) is among the first three concerns Romania has to deal with, besides ‘air pollution’ (67.9%) and ‘deforestation’ (63.7%). Moreover, the study highlights the existence of the behavior toward the selective waste collection among students (plastic – 60.3%, paper – 57.8%, and glass – 55.3%). although there are some areas (e.g., selectively collecting used cooking oil or printer toner, their level of knowledge regarding the color code for the recycling bins, etc.) that students still need to be familiarized with through different campaigns, trainings, courses, etc. The results can be used in the development of institutional strategies or of strategic documents targeting environmental protection and sustainable development.
ICD-11 Posttraumatic Stress Disorder and Complex PTSD Among Hospital Medical Workers in China: Impacts of Wenchuan Earthquake Exposure, Workplaces, and Sociodemographic Factors
Authors: Li Sijian , Guo Chunlan , Chan Sunshine S. S.
Published: January 2022

Background

Previous studies address posttraumatic stress disorder (PTSD) following disasters as a public health issue. However, few studies investigate the long-term effect of disaster exposure on PTSD among hospital medical workers (HMWs).

Objectives

This study aimed to study the prevalence of ICD-11 PTSD and complex PTSD (CPTSD) among exposed and non-exposed HMWs 11 years after the Wenchuan earthquake in China, to identify the factors associated with PTSD and CPTSD scores, and to examine the factor structures of PTSD and CPTSD models.

Methods

A cross-sectional study was conducted using a self-administered online questionnaire. Two thousand fifty-nine valid samples were collected from four hospitals in 2019. Descriptive statistical analysis, multivariate regression models, and confirmatory factor analysis (CFA) were performed.

Results

The prevalence of PTSD and CPTSD was 0.58 and 0.34%, respectively. The unexposed group reported higher PTSD and CPTSD scores than the exposed group. The type of workplace and marital status were significantly associated with the PTSD and CPTSD scores of HMWs. The CFA results indicate that both the correlated first-order model and the correlated two-layer model were a good fit to explain the structure of PTSD and CPTSD.

Conclusion

These findings suggest that few HMWs who were exposed to the Wenchuan earthquake suffered from PTSD or CPTSD 11 years following the disaster. However, psychological support was still necessary for all HMWs, especially for unmarried HMWs who were Working in smaller hospitals. Further research is required to analyze mental health status using ICD-11 PTSD and CPTSD to provide ongoing evidence to help HWMs cope effectively with the challenges of future disasters.
An Updated Evaluation of the Dichotomous Link Between Creativity and Mental Health
Authors: Zhao Rongjun , Tang Zhiwen , Lu Fang , Xing Qiang , Shen Wangbing
Published: January 2022

The theory of the mad genius, a popular cultural fixture for centuries, has received widespread attention in the behavioral sciences. Focusing on a longstanding debate over whether creativity and mental health are positively or negatively correlated, this study first summarized recent relevant studies and meta-analyses and then provided an updated evaluation of this correlation by describing a new and useful perspective for considering the relationship between creativity and mental health. Here, a modified version of the dual-pathway model of creativity was developed to explain the seemingly paradoxical relationship between creativity and mental health. This model can greatly enrich the scientific understanding of the so-called mad genius controversy and further promote the scientific exploration of the link between creativity and mental health or psychopathology.
Millions of Bangladeshi Children Missed Their Scheduled Vaccination Amidst COVID-19 Pandemic
Authors: Hanifi Sayed Manzoor Ahmed , Jahan Nujhat , Sultana Nazia , Hasan Sharif-Al , Paul Ashish , Reidpath Daniel D.
Published: January 2022

The Government of Bangladesh imposed a movement control order as a mass quarantine strategy to control the outbreak of coronavirus disease 2019 (COVID-19). Adherence to the home quarantine may put children at risk by missing routine vaccination. In this study, we investigated the impact of COVID-19 on child routine immunization in a rural area of Bangladesh and consider the broader implications. Data for this study comes from the Chakaria Health and Demographic Surveillance System (HDSS) of icddr,b with a population of 90,000 people residing in 16,000 households in 49 villages in a rural, coastal area of Southeast Bangladesh. We used an explanatory sequential mixed methods design which involved two phases between March 1, 2020, and May 31, 2020: first, we observed 258 outreach sessions of 86 EPI centers. We calculated the number of Expanded Program on Immunization (EPI) outreach sessions suspended and the number of children who missed their routine vaccination due to the COVID-19. We extrapolated the number of Bangladeshi children who missed their routine vaccination using Chakaria HDSS observations. Secondly, we conducted in-depth interviews to explain the quantitative results. The EPI outreach session (EOS) declined to 74.42% (95% CI 63.97–83.22), 10.45% (95% CI 5.00–18.94), and 3.45% (95% CI 1.00–9.75) from 2019 levels in March, April, and May 2020, respectively. By extrapolation, in Bangladesh, between March and May 2020, 3.2 million children missed their scheduled vaccination compared to 2019. Results from in-depth interviews showed that the unwillingness of villagers to hold EOS and the absenteeism of the vaccinators due to social distancing recommendations and lack of personal safety measures were the main reasons for the discontinuation of the EOS. Resuming EPI outreach sessions and introducing a special catch-up program is essential to prevent future outbreaks and deaths due to vaccine-preventable diseases in Bangladesh and the countries where children missed their routine vaccination due to COVID-19. This health system failure should be considered a factor in all future pandemic preparedness plans.
The Decline and Fall of Materia Medica and the Rise of Pharmacology and Therapeutics in Veterinary Medicine
Authors: Lees Peter , Bäumer Wolfgang , Toutain Pierre-Louis
Published: January 2022

Materia Medica is a Latin term, relating to the history of pharmacy. It describes the sources (vegetable, animal and mineral), nature, preparation, and properties of substances or mixtures of substances, which were used as remedies for the treatment of diseases. Bourgelat authored the first veterinary Materia Medica book. This review describes the evolution and ultimate downfall of Materia Medica concepts and practices. Its survival for more than two millennia reflected the impact of religion and dogmas on therapy. The consignment of Materia Medica to history was signified by publication of the first modern book of veterinary pharmacology and therapeutics by Meyer Jones in 1953. Previously, the dominance of Materia Medica was linked to an hippiatry culture, which was shared with farriers and quacks. The Pasteurian and pharmacological revolutions of the second half of the nineteenth century led to its gradual abandonment. This review explains why the existence of authentically active substances, such as opioid analgesics, cardiotonics and general anesthetics either were not used for those actions or were badly prescribed, in part because of historical precedence and in part from lack of pathophysiological knowledge to justify rational use. The modern concept of dosage, in particular inter-species differences, was not understood. There were also major dogmas, supporting false indications, such as failure to recognize pain as a symptom to be treated, whereas inflammation was only a disease symptom involving excess of activity of the blood system, which had to be vigorously addressed by bleeding and purging. This review covers a well-defined period, ranging from Bourgelat, who wrote the first book of Materia Medica for veterinary studies to the first edition of Meyer Jones textbook in 1953, which marked the end of Materia Medica and the beginning of pharmacology in veterinary medicine.
Reducing Inequities During the COVID-19 Pandemic: A Rapid Review and Synthesis of Public Health Recommendations
Authors: Brown Chloe , Wilkins Katie , Craig-Neil Amy , Upshaw Tara , Pinto Andrew David
Published: January 2022

Objectives: Efforts to contain the COVID-19 pandemic should take into account worsening health inequities. While many public health experts have commented on inequities, no analysis has yet synthesized recommendations into a guideline for practitioners. The objective of this rapid review was to identify the areas of greatest concern and synthesize recommendations.

Methods: We conducted a rapid systematic review (PROSPERO: CRD42020178131). We searched Ovid MEDLINE, Embase, PsycINFO, CINAHL and Cochrane Central Register of Controlled Trials databases from December 1, 2019 to April 27, 2020. We included English language peer-reviewed commentaries, editorials, and opinion pieces that addressed the social determinants of health in the context of COVID-19.

Results: 338 articles met our criteria. Authors represented 81 countries. Income, housing, mental health, age and occupation were the most discussed social determinants of health. We categorized recommendations into primordial, primary, secondary and tertiary prevention that spoke to the social determinants of COVID-19 and equity.

Conclusion: These recommendations can assist efforts to contain COVID-19 and reduce health inequities during the pandemic. Using these recommendations, public health practitioners could support a more equitable pandemic response.

Systematic Review Registration: PROSPERO, CRD42020178131.
Nonlinear Robust Control on Yaw Motion of a Variable-Speed Unmanned Aerial Helicopter under Multi-Source Disturbances
Authors: Tang Peng , Dai Yuehong , Chen Junfeng
Published: January 2022

This paper studies the multi-source disturbances attenuation problem on the yaw motion of unmanned aerial helicopter with a variable-speed rotor. The yaw motion subsystem dominated by an electrically-driven tail rotor is firstly introduced, and its trajectory accuracy requires particularly close attention. To this end, we establish a fourth-order yaw error dynamic equation; subsequently, a nonlinear robust control scheme based on optimal H∞ principle is developed, consisting of laws of virtual functions, parameter estimation and a compensation signal. The novelty of this scheme lies in unifying the techniques to deal with the uncertain parameters, noise perturbations, actuator output fault and external airflow turbulence into a simple framework. Stability analysis guarantees that the yaw closed-loop system has the predefined performance of disturbance suppression in the sense of a finite L2-gain. Comparison results with the extended state observer based backstepping controller verify the effectiveness and superior performance of proposed scheme in an aircraft prototype.
Aerodynamic Shape Optimisation of a Camber Morphing Airfoil and Noise Estimation
Authors: Valldosera Martinez Robert , Afonso Frederico , Lau Fernando
Published: January 2022

In order to decrease the emitted airframe noise by a two-dimensional high-lift configuration during take-off and landing performance, a morphing airfoil has been designed through a shape design optimisation procedure starting from a baseline airfoil (NLR 7301), with the aim of emulating a high-lift configuration in terms of aerodynamic performance. A methodology has been implemented to accomplish such aerodynamic improvements by means of the compressible steady RANS equations at a certain angle of attack, with the objective of maximising its lift coefficient up to equivalent values regarding the high-lift configuration, whilst respecting the imposed structural constraints to guarantee a realistic optimised design. For such purposes, a gradient-based optimisation through the discrete adjoint method has been undertaken. Once the optimised airfoil is achieved, unsteady simulations have been carried out to obtain surface pressure distributions along a certain time-span to later serve as the input data for the aeroacoustic prediction framework, based on the Farassat 1A formulation, where the subsequent results for both configurations are post-processed to allow for a comparative analysis. Conclusively, the morphing airfoil has proven to be advantageous in terms of aeroacoustics, in which the noise has been reduced with respect to the conventional high-lift configuration for a comparable lift coefficient, despite being hampered by a significant drag coefficient increase due to stall on the morphing airfoil’s trailing edge.
Non-Chemical Approaches to Control Postharvest Gray Mold Disease in Bell Peppers
Authors: Krasnow Charles , Ziv Carmit
Published: January 2022

Bell pepper (Capsicum annuum) is a widely grown vegetable crop that is nutritious and flavorful and economically important for growers worldwide. A significant limiting factor in the postharvest storage and long-distance transport of peppers is gray mold caused by Botrytis cinerea. The pathogen is widespread in nature, highly aggressive, and able to cause disease at cool refrigerated temperatures during transport and storage. Fungicides have been relied on in the past to reduce bell pepper rots in storage; however, concern over residues on the fruit and environmental degradation have heightened the importance of natural and generally recognized as safe (GRAS) solutions that effectively limit disease. Essential oils, plant extracts, inorganic chemicals, biocontrols, defense activators, hot water treatments, and modified storage conditions have been tested to reduce losses from gray mold. Despite significant amounts of research on natural methods of control of B. cinerea postharvest, research specific to gray mold in peppers is limited. The objective of this review is to summarize the research conducted with environmentally friendly alternatives to chemical fungicides to control this important pathogen of peppers postharvest. To ensure a steady supply of healthy and nutritious produce, more research is needed on the development, use, and application of non-hazardous Botrytis control methods. Until an effective solution is found, using a combined approach including environmental controls, sanitation, and GRAS products remain paramount to limit Botrytis fruit rot of peppers postharvest.
Genome-Wide Association Mapping for Seed Weight in Soybean with Black Seed Coats and Green Cotyledons
Authors: Jo Hyun , Lee Ji Yun , Lee Jeong-Dong
Published: January 2022

The yield of soybean (Glycine max (L.) Merr.) is based on several components, such as the number of plants per unit area, pod number per plant, number of nodes, and seed weight. Additionally, the hundred-seed weight (HSW) is an important component affecting soybean yield. The HSW trait can determine soy products meant for human consumption. In this study, we conducted genome-wide association studies with 470 accessions of black seed coats with green cotyledons and applied an online tool with publicly available genome sequencing data. The objective of the study was to identify the genomic regions in the soybean genome associated with seed weight and to identify the candidate genes in linkage disequilibrium blocks where the most significant SNPs were located. This study identified significant SNPs for seed weight on chromosomes 2 and 16. Furthermore, this study indicated that GmCYP78A57 (Glyma.02G119600) encoded a cytochrome P450 monooxygenase may be a possible candidate gene for controlling the seed size in soybean. We assumed that another gene on chromosome 16 may play the important role of a small additive genetic effect to reduce seed size along with GmCYP78A57. An online tool was used to identify 12 allelic variations of GmCYP78A57 with publicly available genomic sequence data. The HSW of 45 accessions having a missense mutation from the Germplasm Resources Information Network ranged from 4.4 to 17.6 g. In addition, 19 accessions were shown to be less than 10.0 g of HSW. This information can provide for the development of molecular markers to use in soybean breeding programs to release new cultivars with increased or decreased seed weight.
Bark Gnawing by Rodents in Orchards during the Growing Season—Can We Detect Relation with Forest Damages?
Authors: Suchomel Josef , Šipoš Jan , Ouředníčková Jana , Skalský Michal , Heroldová Marta
Published: January 2022

Rodent bark gnawing is common during winter in times of low or unavailable food supply. During the growing season, it is a rare phenomenon, but can occur due to low food supply and/or poor food quality resulting from climatic influence. We evaluated this unusual damage of apple tree (Malus domestica) orchards by rodents in two localities in the Czech Republic. In 2019, 24% of trees in the orchard in Brno were gnawed by the common vole (Microtus arvalis) population. Damage in the Holovousy orchard by water vole (Arvicola amphibius) was inspected in 2020 and showed that 15% of trees were damaged with massive root gnawing. These findings were compared with gnawing in forest beech plantations in 2019, with up to 13% of trees damaged. Three rodent species (Microtus agrestis, M. arvalis and Clethrionomys glareolus) participated in the damage. Detailed description of the various vole species’ damage in vegetation period, which somehow differs from winter time damage, may help in the future in early recognition and early application of plant protection in times when this phenomenon may reappear.
Accumulation Patterns of Six Pyrethrin Compounds across the Flower Developmental Stages—Comparative Analysis in Six Natural Dalmatian Pyrethrum Populations
Authors: Grdiša Martina , Jeran Nina , Varga Filip , Klepo Tatjana , Ninčević Tonka , Šatović Zlatko
Published: January 2022

Pyrethrin is a specialized metabolite of Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip.), Asteraceae, known worldwide as an effective bioinsecticide. It consists of six active compounds: Pyrethrin I and II, cinerin I and II, and jasmolin I and II. Pyrethrin accumulates mainly in the flower heads and its content depends on numerous factors, such as the flower developmental stage. This study aims to investigate the accumulation patterns of six pyrethrin compounds in the flower heads of Dalmatian pyrethrum over the eight developmental stages (FS1 to FS8), and to make a comparison in six natural populations. Ultrasound assisted extraction was used to extract the pyrethrin, while qualitative and quantitative analysis was performed by High performance liquid chromatography. The accumulation patterns of different pyrethrin compounds were generally similar and also synchronous between different populations, while the pyrethrin I/pyrethrin II ratio showed irregular patterns. In all populations studied, the highest increase of all compounds was observed from FS1 to FS2. Their concentration continuously increased, reaching the highest values at FS4 stage (2–5 rows of open disc flowers), and generally decreased gradually towards FS6 or FS7 and then stagnated until the FS8 stage. Despite the very similar accumulation pattern of pyrethrin compounds in the different populations, the significant differences in their content suggest a different genetic background. Knowledge of the dynamics of pyrethrin compounds accumulation across flower development stages is valuable for determining the optimal harvest time of pyrethrum flower heads.
Myostatin Knockout Regulates Bile Acid Metabolism by Promoting Bile Acid Synthesis in Cattle
Authors: Wu Di , Gu Mingjuan , Wei Zhuying , Bai Chunling , Su Guanghua , Liu Xuefei , Zhao Yuefang , Yang Lei , Li Guangpeng
Published: January 2022

Myostatin (MSTN) is a major negative regulator of skeletal muscle mass and causes a variety of metabolic changes. However, the effect of MSTN knockout on bile acid metabolism has rarely been reported. In this study, the physiological and biochemical alterations of serum in MSTN+/− and wild type (WT) cattle were investigated. There were no significant changes in liver and kidney biochemical indexes. However, compared with the WT cattle, lactate dehydrogenase, total bile acid (TBA), cholesterol, and high-density lipoprotein (HDL) in the MSTN+/− cattle were significantly increased, and glucose, low-density lipoprotein (LDL), and triglycerides (TG) were significantly decreased, indicating that MSTN knockout affected glucose and lipid metabolism and total bile acids content. Targeted metabolomic analysis of the bile acids and their derivatives was performed on serum samples and found that bile acids were significantly increased in the MSTN+/− cattle compared with the WT cattle. As the only bile acid synthesis organ in the body, we performed metabolomic analysis on the liver to study the effect of MSTN knockout on hepatic metabolism. Metabolic pathway enrichment analysis of differential metabolites showed significant enrichment of the primary bile acid biosynthesis and bile secretion pathway in the MSTN+/− cattle. Targeted metabolomics data further showed that MSTN knockout significantly increased bile acid content in the liver, which may have resulted from enhanced bile acid synthesis due to the expression of bile acid synthesis genes, cholesterol 7 alpha-hydroxylase (CYP7A1) and sterol 27-hydroxylase (CYP27A1), and upregulation in the liver of the MSTN+/− cattle. These results indicate that MSTN knockout does not adversely affect bovine fitness but regulates bile acid metabolism via enhanced bile acid synthesis. This further suggests a role of MSTN in regulating metabolism.
The Social Life of Pigs: Changes in Affiliative and Agonistic Behaviors following Mixing
Authors: O’Malley Carly I. , Steibel Juan P. , Bates Ronald O. , Ernst Catherine W. , Siegford Janice M.
Published: January 2022

This study investigated potentially affiliative behaviors in grow-finish pigs, how these behaviors changed over time and their relationship to agonistic behaviors. A total of 257 Yorkshire barrows were observed for agonistic (reciprocal fights, attacks) and affiliative (nosing, play, non-agonistic contact) behaviors after mixing (at 10 weeks of age), and weeks 3, 6, and 9 after mix. The least square means of affiliative behaviors were compared across time points. Relationships among affiliative and agonistic behaviors were assessed using generalized linear mixed models. Non-agonistic contact with conspecifics increased until week 6 then remained stable between weeks 6 and 9. Nosing was highest at mix, then decreased in the following weeks. Play was lowest at mix and highest at week 3. Affiliative behaviors were negatively related with aggression at mix (p < 0.001). Pigs who engaged in play and nosing behaviors were more likely to be involved in agonistic interactions in the weeks after mixing (p < 0.05), while pigs engaging in non-agonistic contact were less likely to be involved in agonistic interactions (p < 0.001). There appear to be relationships between affiliative and agonistic behaviors in pigs, with contact being the most predictive of less aggression. Future studies could focus on promoting positive non-agonistic contact in unfamiliar pigs as a way to mitigate aggressive interactions.
Management of Chronic Congestive Heart Failure Caused by Myxomatous Mitral Valve Disease in Dogs: A Narrative Review from 1970 to 2020
Authors: Bagardi Mara , Zamboni Viola , Locatelli Chiara , Galizzi Alberto , Ghilardi Sara , Brambilla Paola G.
Published: January 2022

The treatment of chronic congestive heart failure (CHF), secondary to myxomatous mitral valve disease (MMVD) in dogs, has considerably changed in the last fifty years. An analysis of the literature concerning the therapy of chronic CHF in dogs affected by MMVD is not available, and it is needed. Narrative reviews (NRs) are aimed at identifying and summarizing what has been previously published, avoiding duplications, and seeking new study areas that have not yet been addressed. The most accessible open-access databases, PubMed, Embase, and Google Scholar, were chosen, and the searching time frame was set in five decades, from 1970 to 2020. The 384 selected studies were classified into categories depending on the aim of the study, the population target, the pathogenesis of MMVD (natural/induced), and the resulting CHF. Over the years, the types of studies have increased considerably in veterinary medicine. In particular, there have been 43 (24.29%) clinical trials, 41 (23.16%) randomized controlled trials, 10 (5.65%) cross-over trials, 40 (22.60%) reviews, 5 (2.82%) comparative studies, 17 (9.60%) case-control studies, 2 (1.13%) cohort studies, 2 (1.13%) experimental studies, 2 (1.13%) questionnaires, 6 (3.40%) case-reports, 7 (3.95%) retrospective studies, and 2 (1.13%) guidelines. The experimental studies on dogs with an induced form of the disease were less numerous (49–27.68%) than the studies on dogs affected by spontaneous MMVD (128–72.32%). The therapy of chronic CHF in dogs has considerably changed in the last fifty years: in the last century, some of the currently prescribed drugs did not exist yet, while others had different indications.
Characterization of Testicular Tumor Lesions in Dogs by Different Ultrasound Techniques
Authors: Orlandi Riccardo , Vallesi Emanuela , Boiti Cristiano , Polisca Angela , Bargellini Paolo , Troisi Alessandro
Published: January 2022

In this retrospective study, we assessed the accuracy of different blood flow imaging in diagnosing testicular tumor types in dogs. We recruited 27 dogs with leydigomas (14), seminomas (eight), sertoliomas (six), and mixed cells (five) confirmed histopathologically. In intact dogs, Pampiniform plexus and marginal arteries were scanned through pulsed Doppler. Blood flow and presence of intralesional/perilesional arteries were assessed by color and power Doppler, B-flow, and contrast-enhanced ultrasound. Tumor types did not differ by B-Mode ultrasonography characters. Pampiniform and testicular arteries of sertoliomas had higher (p < 0.05) pulsatility and resistive indexes. The proportion of leydigomas with a perilesional and/or perilesional/intralesional blood flow pattern detected by color and pulsed Doppler and B-flow was higher (p < 0.05) than that of the other tumors counted together. This resulted in a sensitivity of 81.8%, 83.3%, and 85.7%, a specificity of 76.5%, 56.3%, and 73.7%, and a correct classification rate of 78.6%, 67.9%, and 78.8%, respectively. While contrast enhanced ultrasound was highly effective in detecting all tumors, qualitative and quantitative parameters did not contribute to their differential diagnosis. In conclusion, results indicate that different testicular tumor types of dogs have subtly different vascular patterns, a condition that could help in identifying leydigomas.
Effects of Cage and Floor Rearing Systems on the Metabolic Components of the Uropygial Gland in Ducks
Authors: Liu Hehe , Qi Jiawen , Yang Qinglan , Tang Qian , Qi Jingjing , Li Yanying , Wang Jiwen , Han Chunchun , Li Liang
Published: January 2022

Background: As a unique skin derivative of birds, the uropygial gland has a potential role in maintaining feather health and appearance. Cage-reared ducks usually have a worse feather condition than floor-reared ducks. We suspected that the metabolic components in the uropygial gland might play a vital role in their feather conditions. Methods: Herein, the uropygial glands of floor- and cage-reared ducks were weighed, and a nontargeted metabolic analysis was performed. Results: At 20 weeks of age, the relative weight of floor-reared duck uropygial glands was significantly higher than that of cage-reared ducks, indicating that the floor rearing system is better for inducing the development of uropygial glands. The nontargeted metabolic data revealed 1190 and 1149 differential metabolites under positive and negative ion modes, respectively. Among them, 49 differential metabolites were annotated between the two rearing systems. Three sulfur-containing amino acids, namely, 2-ketobutyric acid, L-aspartate-semialdehyde, and N-formyl-L-methionine, and some lipids, including inositol and sphingosine, might be responsible for the changes in plumage appearance among the various rearing conditions. Conclusions: The results of our study revealed the differences in the metabolic components of the uropygial gland in ducks reared under different rearing systems and found metabolic components to be possibly responsible for the poor feather condition of caged ducks.
Rapid-Erection Backstepping Tracking Control for Electrohydraulic Lifting Mechanisms of Launcher Systems
Authors: Li Lan , Jiang Yi , Yang Xiaowei , Yao Jianyong
Published: January 2022

Uncertainties and disturbances widely exist in electrohydraulic lifting mechanisms of launcher systems, which may worsen the rapid-erection tracking accuracy and even make the system unstable. To deal with the issue, an asymptotic tracking control framework is developed for electrohydraulic lifting mechanisms of launcher systems. Firstly, the dynamic equations and state-space forms of the electrohydraulic lifting mechanism are modeled. Based on the system model, a nonlinear rapid-erection robust controller is constructed to achieve the improvement of the system control performance, in which a nonlinear feedback term is employed to remove the effects of uncertainties and disturbances on tracking performance. Compared to the existing results, the asymptotic tracking stability of the closed-loop system can be assured based on the Lyapunov theory analysis. In the end, the simulation example of an actual electrohydraulic lifting mechanism of the launcher system is done to validate the effectiveness with the proposed controller.