bluuebunny/crossref_metadata_2025 huggingface dataset:
Association between Peptic Ulcer Disease and Osteoporosis: The Population-Based Longitudinal Cohort Study in Korea
Authors: Yoon Phill Hoon , An Sang Joon , Jeong Seok-Hoo , Yang Yun-Jung , Hong Yeon-pyo
Published: August 2019

Objectives: The association between peptic ulcer disease (PUD) and osteoporosis remains unclear. We investigated the association between PUD and osteoporosis by classifying individuals based on gender in a prospective study on 10,030 adults in Korea at a 12-year follow-up examination. Design and setting: The baseline survey of the Ansung–Ansan cohort studies was conducted from May 2001 to February 2003, and 10,030 participants (5018 from the Ansung study and 5012 from the Ansan study) completed the examination. Primary outcome measures: The risk of developing osteoporosis was higher in both men and women in the PUD group than in the control group. Results: At the 12-year follow-up, osteoporosis had developed in 11.1% (21/189) and 29.9% (56/187) of men and women in the PUD group, respectively. Meanwhile, in the control group, 4.8% (70/1464) and 16.5% (271/1639) of men and women, respectively, were presented with osteoporosis. The incidence rates per 1000 person–years were 20.5% and 68.5% in men and women in the PUD group and 11.2% and 42.3% of men and women in the control group, respectively. The risk of developing osteoporosis was higher in both men and women in the PUD group than in the control group (men: hazard ratio [HR] = 1.72, 95% confidence interval [CI] = 1.02–2.92; women: HR = 1.62, 95% CI = 1.20–2.18). Conclusion: The risk of developing osteoporosis was significantly higher in both men and women in the PUD group than in the control group.
Community Advantage and Individual Self-Efficacy Promote Disaster Preparedness: A Multilevel Model among Persons with Disabilities
Authors: Adams Rachel M. , Eisenman David P. , Glik Deborah
Published: August 2019

Disaster preparedness initiatives are increasingly focused on building community resilience. Preparedness research has correspondingly shifted its attention to community-level attributes that can support a community’s capacity to respond to and recover from disasters. While research at the community level is integral to building resilience, it may not address the specific barriers and motivators to getting individuals prepared. In particular, people with disabilities are vulnerable to disasters, yet research suggests that they are less likely to engage in preparedness behaviors. Limited research has examined what factors influence their ability to prepare, with no studies examining both the individual and community characteristics that impact these behaviors. Multilevel modeling thus offers a novel contribution that can assess both levels of influence. Using Los Angeles County community survey data from the Public Health Response to Emergent Threats Survey and the Healthy Places Index, we examined how social cognitive and community factors influence the relationship between disability and preparedness. Results from hierarchical linear regression models found that participants with poor health and who possessed activity limitations engaged in fewer preparedness behaviors. Self-efficacy significantly mediated the relationship between self-rated health and disaster preparedness. Living in a community with greater advantages, particularly with more advantaged social and housing attributes, reduced the negative association between poor self-rated health and preparedness. This study highlights the importance of both individual and community factors in influencing people with disabilities to prepare. Policy and programming should therefore be two-fold, both targeting self-efficacy as a proximal influence on preparedness behaviors and also addressing upstream factors related to community advantage that can create opportunities to support behavioral change while bolstering overall community resilience.
Construction and Validation of an Analytical Grid about Video Representations of Suicide (“MoVIES”)
Authors: Gauld Christophe , Wathelet Marielle , Medjkane François , Pauwels Nathalie , Bougerol Thierry , Notredame Charles-Edouard
Published: August 2019

Background. Exposure to fictional suicide scenes raises concerns about the risk of suicide contagion. However, researchers and clinicians still lack empirical evidence to estimate this risk. Here, we propose a theory-grounded tool that measures properties related to aberrant identification and suicidal contagion of potentially harmful suicide scenes. Methods. The items of the Movies and Video: Identification and Emotions in reaction to Suicide (MoVIES) operationalize the World Health Organization’s recommendations for media coverage of suicide, and were adapted and completed with identification theory principles and cinematographic evidence. Inter-rater reliability (Cohen’s kappa) and internal consistency (Cronbach’s alpha) were estimated and optimized for two series of 19 and 30 randomly selected movies depicting a suicide scene. The validity of the scale in predicting identification with the suicidal character was tested in nine unknowledgeable participants who watched seven suicide movie scenes each. Results. The MoVIES indicated satisfying psychometric properties with kappas measured at 0.7 or more for every item and a global internal consistency of [α = 0.05]. The MoVIES score significantly predicted participants’ strength of identification independently from their baseline empathy ((β = 0.20), p < 0.05). Conclusions. The MoVIES is available to scholars as a valid, reliable, and useful tool to estimate the amount of at-risk components of fictional suicidal behavior depicted in films, series, or television shows.
Popularity and Friendships and Their Relationship to Physical Activity Before and After Transition to a Higher School Grade
Authors: Swanson Kenda C. , Nettel-Aguirre Alberto , McCormack Gavin R.
Published: August 2019

Background This study investigated the relationships between children’s friendship ties and their physical activity (PA) both before and after their transition to a new school year. Methods In 2011–2012, children in grades 5–8 attending a Canadian urban middle-school completed web-based health and friendship surveys two times before (“pre-transition”) and three times after (“post-transition”) they moved up in school grade. Cross-sectional associations between an average daily frequency of ≥60 min/day of moderate-to-vigorous intensity PA (MVPA) and characteristics of children’s friendships were estimated for pre-transition (n = 191) and post-transition (n = 255) data. Sociodemographic-adjusted linear regression (β) estimated associations between a child’s MVPA and friendship characteristics. Results We found positive associations between a child’s MVPA and the average MVPA of their friends at post-transition only (β = 0.61, 95% CI 0.10 to 1.13) and the number of sent friendships at pre-transition (β = 0.03, 95% CI 0.01 to 0.05) and post-transition (β = 0.02, 95% CI 0.01 to 0.04). A statistically significant interaction between popularity and friends’ average PA at pre-transition was also found. Conclusions The PA of friends and the number of school friends that a child identified are positively associated with MVPA. The estimated associations between MVPA and aspects of children’s friendships are similar for boys and girls.
An Altered DNA Methylation Status in the Human Umbilical Cord Is Correlated with Maternal Exposure to Polychlorinated Biphenyls
Authors: Eguchi Akifumi , Nishizawa-Jotaki Shino , Tanabe Hiromi , Rahmutulla Bahityar , Watanabe Masahiro , Miyaso Hidenobu , Todaka Emiko , Sakurai Kenichi , Kaneda Atsushi , Mori Chisato
Published: August 2019

Maternal exposure to polychlorinated biphenyls (PCBs) results in abnormal fetal development, possibly because of epigenetic alterations. However, the association between PCB levels in cord serum with fetal DNA methylation status in cord tissue is unclear. This study aims to identify alterations in DNA methylation in cord tissue potentially associated with PCB levels in cord serum from a birth cohort in Chiba, Japan (male neonates = 32, female neonates = 43). Methylation array analysis identified five sites for female neonates (cg09878117, cg06154002, cg06289566, cg12838902, cg01083397) and one site for male neonates (cg13368805) that demonstrated a change in the methylation degree. This result was validated by pyrosequencing analysis, showing that cg06154002 (tudor domain containing 9: TDRD9) in cord tissue from female neonates is significantly correlated with total PCB levels in cord serum. These results indicate that exposure to PCBs may alter TDRD9 methylation levels, although this hypothesis requires further validation using data obtained from female neonates. However, since the present cohort is small, further studies with larger cohorts are required to obtain more data on the effects of PCB exposure and to identify corresponding biomarkers.
Effects of Tidal Scenarios on the Methane Emission Dynamics in the Subtropical Tidal Marshes of the Min River Estuary in Southeast China
Authors: Huang Jiafang , Luo Min , Liu Yuxiu , Zhang Yuxue , Tan Ji
Published: August 2019

In order to accurately estimate the effects of tidal scenarios on the CH4 emission from tidal wetlands, we examined the CH4 effluxes, dissolved CH4 concentrations, and environmental factors (including in situ pH, Eh and electrical conductivity, porewater SO42−, NO3−, and NH4+) during inundation and air-exposure periods in high- and low-tide seasons in the Min River Estuary in southeast China. By applying static and floating chambers, our results showed that the CH4 effluxes during the inundation periods were relatively constant and generally lower than those during the air-exposed periods in both seasons. When compared, the CH4 effluxes during the air-exposed periods were significantly higher in the high-tide season than those in the low-tide season. In contrast, CH4 effluxes during the inundation periods were significantly lower in the high-tide season than those in the low-tide season. During the inundation periods, dissolved CH4 concentrations were inversely proportional to in situ Eh. Under air-exposed conditions, CH4 effluxes were proportional to in situ pH in both seasons, while the dissolved CH4 concentrations were negatively correlated with the porewater SO42− concentrations in both seasons. Our results highlighted that CH4 effluxes were more dynamic between inundation and air-exposure periods compared to low- and high-tide seasons.
Polymorphic Variants of V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B (rs13041247 and rs11696257) and Risk of Non-Syndromic Cleft Lip/Palate: Systematic Review and Meta-Analysis
Authors: Imani Mohammad Moslem , Lopez-Jornet Pia , Pons-Fuster López Eduardo , Sadeghi Masoud
Published: August 2019

Background: Non-syndromic cleft lip/palate (NSCL/P) has an etiology, including both genetic and environmental factors. Herein, we evaluated the association of rs13041247 and rs11696257 v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) polymorphisms with the risk of NSCL/P in a meta-analysis. Methods: The PubMed/Medline, Scopus, Cochrane Library, Web of Science, and HuGE Navigator databases were systematically searched to retrieve relevant articles published up to January 2019. The Newcastle–Ottawa scale was applied for quality evaluation of retrieved articles. The 95% confidence interval (CI) and crude odds ratio (OR) were calculated for each study using the Review Manager 5.3 software to show the association between MAFB polymorphisms and risk of NSCL/P. The comprehensive meta-analysis 2.0 software was used to calculate the publication bias. In addition, sensitivity analysis was carried out to show the stability of results. Results: Of 102 articles retrieved from the databases, 10 articles were analyzed in this meta-analysis. Ten articles, including eleven studies reporting rs13041247 MAFB polymorphism, included 3082 NSCL/P patients and 4104 controls. Three studies that reported rs11696257 MAFB polymorphism involved 845 NSCL/P patients and 927 controls. The rs11696257 MAFB polymorphism was not associated with the risk of NSCL/P, but the CC and TC genotypes of rs13041247 polymorphism were associated with the risk of NSCL/P. Nevertheless, the C allele and CC and TC genotypes were associated with a significant decline in the risk of NSCL/P in population-based studies. Conclusions: The results of this meta-analysis demonstrated that the risk of NSCL/P was related to rs13041247 polymorphism, not rs11696257 MAFB polymorphism. Well-designed studies are required to assess the interaction of MAFB and other genes with environmental factors in different ethnic groups.
Measuring Work-Related Functioning Using the Work Rehabilitation Questionnaire (WORQ)
Authors: Finger Monika E. , Escorpizo Reuben , Tennant Alan
Published: August 2019

The assessment of work-related functioning is a key process in vocational rehabilitation to identify specific domains of disability that can be considered within return to work strategies. The Work Rehabilitation Questionnaire (WORQ) was developed to evaluate work-related functioning based on the International Classification of Functioning, Disability, and Health (ICF) framework and is available in different languages. The aim of this study was to assess the French version of the WORQ using item response theory to further validate the scale. Rasch analysis of WORQ and the WORQ-BRIEF (a brief version of the WORQ) was performed using a calibration sample of 221 persons with musculoskeletal injuries. A four-testlet solution indicated the unidimensionality of WORQ, with no differential item functioning for age, education, physical job demands, and injury severity. Reliability was 0.969 and 0.918 for WORQ and WORQ-BRIEF, respectively. The minimal detectable change was calculated to be 4.2% of its operational range for WORQ and 8.5% for WORQ-BRIEF. Consequently, the French version of WORQ can be considered a good measure of work-related functioning in musculoskeletal conditions. WORQ can be used in rehabilitation practice to comprehensively identify the disability and guide clinical decision making and intervention planning. Further studies are needed to evaluate the psychometric properties of WORQ in other health conditions.
Histamine Is an Inducer of the Heat Shock Response in SOD1-G93A Models of ALS
Authors: Apolloni Savina , Caputi Francesca , Pignataro Annabella , Amadio Susanna , Fabbrizio Paola , Ammassari-Teule Martine , Volonté Cinzia
Published: August 2019

(1) Background: Amyotrophic lateral sclerosis (ALS) is a multifactorial non-cell autonomous disease where activation of microglia and astrocytes largely contributes to motor neurons death. Heat shock proteins have been demonstrated to promote neuronal survival and exert a strong anti-inflammatory action in glia. Having previously shown that the pharmacological increase of the histamine content in the central nervous system (CNS) of SOD1-G93A mice decreases neuroinflammation, reduces motor neuron death, and increases mice life span, here we examined whether this effect could be mediated by an enhancement of the heat shock response. (2) Methods: Heat shock protein expression was analyzed in vitro and in vivo. Histamine was provided to primary microglia and NSC-34 motor neurons expressing the SOD1-G93A mutation. The brain permeable histamine precursor histidine was chronically administered to symptomatic SOD1-G93A mice. Spine density was measured by Golgi-staining in motor cortex of histidine-treated SOD1-G93A mice. (3) Results: We demonstrate that histamine activates the heat shock response in cultured SOD1-G93A microglia and motor neurons. In SOD1-G93A mice, histidine augments the protein content of GRP78 and Hsp70 in spinal cord and cortex, where the treatment also rescues type I motor neuron dendritic spine loss. (4) Conclusion: Besides the established histaminergic neuroprotective and anti-inflammatory effects, the induction of the heat shock response in the SOD1-G93A model by histamine confirms the importance of this pathway in the search for successful therapeutic solutions to treat ALS.
Testing Anti-Biofilm Polymeric Surfaces: Where to Start?
Authors: Cattò Cristina , Cappitelli Francesca
Published: August 2019

Present day awareness of biofilm colonization on polymeric surfaces has prompted the scientific community to develop an ever-increasing number of new materials with anti-biofilm features. However, compared to the large amount of work put into discovering potent biofilm inhibitors, only a small number of papers deal with their validation, a critical step in the translation of research into practical applications. This is due to the lack of standardized testing methods and/or of well-controlled in vivo studies that show biofilm prevention on polymeric surfaces; furthermore, there has been little correlation with the reduced incidence of material deterioration. Here an overview of the most common methods for studying biofilms and for testing the anti-biofilm properties of new surfaces is provided.
Dihydroxy-Substituted Coumarins as Fluorescent Probes for Nanomolar-Level Detection of the 4-Amino-TEMPO Spin Label
Authors: Żamojć Krzysztof , Zdrowowicz Magdalena , Hać Aleksandra , Witwicki Maciej , Rudnicki-Velasquez Paweł Błażej , Wyrzykowski Dariusz , Wiczk Wiesław , Chmurzyński Lech
Published: August 2019

This paper reports on dihydroxycoumarins as fluorescent probes suitable for the detection and determination of the nitroxide radical, namely 4-amino-TEMPO. Since 4-amino-TEMPO is used as a spin label for the detection of various radicals and damage caused by these species, its determination under physiological conditions might help us to understand the mechanism of the oxidative stress. Among different coumarins studied, only dihydroxy-substituted derivatives show high sensitivity, specificity, and selectivity for the nitroxide radical. In this assay, dihydroxy-substituted coumarins under the action of 4-amino-TEMPO show a very fast and significant increase in fluorescence intensity and lifetime. Among them 6,7-dihydroxycoumarin (esculetin) exhibits the strongest fluorescence enhancement (up to 40 times), with an estimated limit of detection equal to 16.7 nM—a significantly lower value when compared with UV-Vis or electron paramagnetic resonance (EPR) spectroscopy. The method is characterized by an easy procedure of sample preparation and very short time of analysis. The mechanism of the interaction between 6,7-dihydroxycoumarin and 4-amino-TEMPO has been examined with the use of a series of complementary techniques, such as steady-state and time-resolved fluorescence spectroscopy, UV-Vis spectroscopy, electron paramagnetic resonance spectroscopy, potentiometric titration, and high-performance liquid chromatography. It has been proven that the only route of the reaction in the system studied is a proton transfer from the molecule of esculetin to the amino group of the nitroxide. Biological studies performed on prostate cancer cells, breast cancer cells, and normal skin fibroblasts revealed significant anticancer properties of 6,7-dihydroxycoumarin, which caused a considerable decrease in the viability and number of cancer cells, and affected their morphology, contrary to normal fibroblasts. Furthermore, the experiment performed on prostate cancer cells showed that fluorescence emission of esculetin is closely related to intracellular pH—the higher pH, the higher observed fluorescence intensity (in accordance with a chemical experiment). On the other hand, the studies performed in different pH levels revealed that when pH of the solution increases, the observed fluorescence intensity enhancement under the action of 4-amino-TEMPO decreases (better sensing properties of esculetin towards the nitroxide in lower pH).
SUSD2 Proteolytic Cleavage Requires the GDPH Sequence and Inter-Fragment Disulfide Bonds for Surface Presentation of Galectin-1 on Breast Cancer Cells
Authors: Patrick Mitch E. , Egland Kristi A.
Published: August 2019

Galectin-1 (Gal-1) is a 14 kDa protein that has been well characterized for promoting cancer metastasis and tumor immune evasion. By localizing to the cancer cell surface, Gal-1 induces T cell apoptosis through binding T cell surface receptors. The transmembrane protein, Sushi Domain Containing 2 (SUSD2), has been previously shown to be required for Gal-1 surface presentation in breast cancer cells. Western immunoblot analysis revealed that SUSD2 is cleaved into two fragments. However, the significance of this cleavage for Gal-1 surface localization has not been investigated. To define the location of cleavage, a mutagenesis analysis of SUSD2 was performed. Our studies demonstrated that SUSD2 is cleaved at its glycine-aspartic acid-proline-histidine (GDPH) amino acid sequence. Generation of a noncleavable SUSD2 mutant (GDPH∆-SUSD2) showed that SUSD2 cleavage was required for SUSD2 and Gal-1 plasma membrane localization. Noncleavable cysteine mutants were also unable to present Gal-1 at the cell surface, further demonstrating that SUSD2 cleavage is required for Gal-1 surface presentation. Treatment with the serine protease inhibitor, Pefabloc SC, inhibited SUSD2 cleavage in a dose dependent manner, suggesting that SUSD2 is cleaved by a serine protease. Therefore, identification and inhibition of this protease may provide a new therapeutic tool for inhibiting SUSD2 and Gal-1′s combined tumorigenic function in breast cancer.
Supercritical Fluid Extract of Angelica sinensis and Zingiber officinale Roscoe Ameliorates TNBS-Induced Colitis in Rats
Authors: Liu Jia , Yu Ling , Mo Nuolan , Lan Hai , Zhang Yan , Liu Xin , Wu Qing
Published: August 2019

Inflammatory bowel disease (IBD) is a worldwide healthcare problem calling for the development of new therapeutic drugs. Angelica sinensis and Zingiber officinale Roscoe are two common dietetic Chinese herbs, which are traditionally used for complementary treatment of gastrointestinal disorders. As bioactive constituents, volatile and pungent substances of these two herbs could be effectively extracted together by supercritical fluid extraction. In this study, the supercritical fluid extract of Angelica sinensis and Zingiber officinale Roscoe (AZ-SFE) was obtained by an optimized extraction process and it was chemically characterized. The anti-inflammatory effect and underlying mechanism of AZ-SFE were evaluated in a lipopolysaccharide (LPS)-induced RAW264.7 cell model and a 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)-induced colitis rat model. AZ-SFE notably inhibited the production of NO in LPS-stimulated macrophages, and it inhibited the proliferation of Concanavalin A (Con A)-induced splenocytes with suppression of the Th1 immune response. In vivo, the study demonstrated that AZ-SFE significantly alleviated disease activity, colonic shortening, macroscopic damage and histological injury of TNBS-treated rats with reduction of oxidative stress, suppression of inflammatory cytokines, and modulation of hepcidin and serum iron. These findings suggested that AZ-SFE may be a promising supplement for current IBD therapy.
Identification and Clinical Validation of a Novel 4 Gene-Signature with Prognostic Utility in Colorectal Cancer
Authors: Ahluwalia Pankaj , Mondal Ashis K. , Bloomer Chance , Fulzele Sadanand , Jones Kimya , Ananth Sudha , Gahlay Gagandeep K. , Heneidi Saleh , Rojiani Amyn M. , Kota Vamsi , Kolhe Ravindra
Published: August 2019

Colorectal cancer (CRC) is a high burden disease with several genes involved in tumor progression. The aim of the present study was to identify, generate and clinically validate a novel gene signature to improve prediction of overall survival (OS) to effectively manage colorectal cancer. We explored The Cancer Genome Atlas (TCGA), COAD and READ datasets (597 samples) from The Protein Atlas (TPA) database to extract a total of 595 candidate genes. In parallel, we identified 29 genes with perturbations in > 6 cancers which are also affected in CRC. These genes were entered in cBioportal to generate a 17 gene panel with highest perturbations. For clinical validation, this gene panel was tested on the FFPE tissues of colorectal cancer patients (88 patients) using Nanostring analysis. Using multivariate analysis, a high prognostic score (composite 4 gene signature—DPP7/2, YWHAB, MCM4 and FBXO46) was found to be a significant predictor of poor prognosis in CRC patients (HR: 3.42, 95% CI: 1.71–7.94, p < 0.001 *) along with stage (HR: 4.56, 95% CI: 1.35–19.15, p = 0.01 *). The Kaplan-Meier analysis also segregated patients on the basis of prognostic score (log-rank test, p = 0.001 *). The external validation using GEO dataset (GSE38832, 122 patients) corroborated the prognostic score (HR: 2.7, 95% CI: 1.99–3.73, p < 0.001 *). Additionally, higher score was able to differentiate stage II and III patients (130 patients) on the basis of OS (HR: 2.5, 95% CI: 1.78–3.63, p < 0.001 *). Overall, our results identify a novel 4 gene prognostic signature that has clinical utility in colorectal cancer.
Effect of Geometry Variability on Transonic Fan Blade Untwist †
Authors: Lu Yaozhi , Lad Bharat , Green Jeff , Stapelfeldt Sina , Vahdati Mehdi
Published: August 2019

Due to manufacturing tolerance and deterioration during operation, fan blades in the same engine exhibit geometric variability. The absence of symmetry will inevitably exacerbate and contribute to the complexities of running geometry prediction as the blade variability is bound to be amplified by aerodynamic and centrifugal loading. In this study, we aim to address the fan blade untwist related phenomenon known as alternate passage divergence (APD). As the name suggests, APD manifests as alternating passage geometry (and hence alternating tip stagger pattern) when the fan stage is operating close to/at peak efficiency condition. APD can introduce adverse influence on fan performance, aeroacoustics behaviour, and high cycle fatigue characteristics of the blade. The main objective of the study is to identify the parameters contributing to the APD phenomenon. In this study, the APD behaviours of two transonic fan blade designs are compared.
Video Games and Collaborative Learning in Education? A Scale for Measuring In-Service Teachers’ Attitudes towards Collaborative Learning with Video Games
Authors: Martín-del-Pozo Marta , García-Valcárcel Muñoz-Repiso Ana , Hernández Martín Azucena
Published: August 2019

Students’ motivation is a fundamental factor in the educational process, and can be facilitated through new methodologies and technologies, including gamification, video games, collaborative learning, or, in particular, the methodology called “collaborative learning with video games” (which is presented and can be understood as the implementation of educational activities in which students have to work together to achieve a goal, and the main resource of the activity is a video game). However, if teachers themselves are not motivated, or if they lack a positive attitude towards implementing these new methodologies, it will be difficult for students to feel motivated when approaching said resources. Therefore, it is important to know what teachers’ attitudes towards them are. The aim of this research is the creation of an attitudes scale towards collaborative learning with video games, aimed at in-service primary school teachers. Different methodological steps were followed that made its construction possible, such as the analysis of items and the verification of their reliability, resulting in a rigorous attitudes scale of 33 items, with a reliability of α = 0.947. This implies that the measurement instrument is validated and allows one to know the attitudes of in-service primary school teachers towards a new methodology related to the implementation of video games in education.
The Effect of Storage-Induced Changes in Ammonia Borane on Hydrogen Release during Its Low-Temperature Thermolysis
Authors: Komova Oxana V. , Netskina Olga V. , Ozerova Anna M. , Odegova Galina V. , Arzumanov Sergei S. , Simagina Valentina I.
Published: August 2019

In this paper, low-temperature solid-state processes of the dehydrogenation of ammonia borane (NH3BH3, AB) samples of different purity are compared under the conditions of isothermal heating at 100 °C, as well as in the course of thermal analysis which was also carried out at different rates of heating. The composition of boron-containing impurities was studied by attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR) and 11B magic angle spinning nuclear magnetic resonance (11B MAS NMR). Accumulation in AB of three- and four-coordinated borate anions upon contact of hydride with air moisture is established. The apparent activation energies were calculated from thermal analysis data, and found to decrease from 174 to 163 kJ/mol as the AB purity decreased from 93% to 79%. This showed itself in a shortening of the induction period during the AB thermolysis under isothermal conditions. The prospects of using the thermal analysis for estimating NH3BH3 reactivity are discussed.
Host Associations of Culex (Melanoconion) atratus (Diptera: Culicidae) and Culex (Melanoconion) pilosus from Florida, USA
Authors: Reeves Lawrence E. , Hoyer Isaiah , Acevedo Carolina , Burkett-Cadena Nathan D.
Published: August 2019

Characterizing the host-use patterns of mosquitoes is an essential component of understanding the transmission dynamics of mosquito-vectored pathogens. The host associations of two species of the medically important Culex subgenus Melanoconion, Culex atratus, and Culex pilosus are unknown or unclear, respectively. Both species have wide neotropical distributions. In the United States of America (USA), Culex pilosus occurs throughout the southeastern coastal plain, while Culex atratus is restricted to the southern Florida Peninsula. Using PCR-based blood meal analysis, we investigated the host associations of Culex atratus and Culex pilosus that were collected from Everglades National Park, Florida, USA We identified the host species of 208 Culex atratus and 168 Culex pilosus. Both species were narrowly associated with reptilian host species, particularly native and non-native lizards of the genus Anolis. Sampled Culex atratus exclusively fed on reptilian hosts, with >99% of blood meals derived from Anolis lizards. Culex pilosus fed predominantly from reptiles, but avian and mammalian blood meals were also detected. Of these, 92% of blood meals were derived from Anolis species. For both species, Anolis sagrei, an invasive exotic lizard in Florida, was the most frequently detected host species. These data indicate that Culex atratus and Culex pilosus are specialists of reptilian hosts, particularly Anolis lizards.
Subterranean Termite Social Alarm and Hygienic Responses to Fungal Pathogens
Authors: Bulmer Mark S. , Franco Bruno A. , Fields Edith G.
Published: August 2019

In social insects, alerting nestmates to the presence of a pathogen should be critical for limiting its spread and initiating social mechanisms of defense. Here we show that subterranean termites use elevated vibratory alarm behavior to help prevent fatal fungal infections. The elevated alarm leads to elevated social hygiene. This requires that termites coalesce so that they can groom each other’s cuticular surfaces of contaminating conidial spores. Groups of 12 Reticulitermes flavipes workers varied in their response when immersed in conidia solutions of nine different strains of Metarhizium. Pathogen alarm displays of short 2–7-second bursts of rapid longitudinal oscillatory movement (LOM), observed over 12 min following a fungal challenge, were positively correlated with the time that workers spent aggregated together grooming each other. The frequency of these LOMs was inversely correlated with fatal fungal infections. The variation in fatalities appeared to be largely attributable to a differential response to Metarhizium brunneum and Metarhizium robertsii in the time spent in aggregations and the frequency of allogrooming. Isolated workers challenged with conidia did not display LOMs, which suggests that the alarm is a conditional social response. LOMs appear to help signal the presence of fungal pathogens whose virulence depends on the level of this emergency alert.
Retinal Blood Velocity and Flow in Early Diabetes and Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy
Authors: Palochak Cherilyn Mae A. , Lee Hee Eun , Song Jessica , Geng Andrew , Linsenmeier Robert A. , Burns Stephen A. , Fawzi Amani A.
Published: August 2019

Using adaptive optics scanning laser ophthalmoscopy (AOSLO), we measured retinal blood velocity and flow in healthy control eyes and eyes of diabetic patients with or without retinopathy. This cross-sectional study included 39 eyes of 30 patients with diabetes (DM) with mild non-proliferative diabetic retinopathy (NPDR) or without retinopathy (DM no DR) and 21 eyes of 17 healthy age-matched controls. Participants were imaged with a commercial optical coherence tomography angiography (OCTA) device (RTVue-XR Avanti) and AOSLO device (Apaeros Retinal Imaging System, Boston Micromachines). We analyzed AOSLO-based retinal blood velocity and flow, and OCTA-based vessel density of the superficial (SCP), deep retinal capillary plexus (DCP), and full retina. Retinal blood velocity was significantly higher in eyes with DM no DR and lower in NPDR across all vessel diameters compared to controls. Retinal blood flow was significantly higher in DM no DR and lower in NPDR in vessel diameters up to 60 μm compared to controls. When comparing flow outliers (low-flow DM no DR eyes and high-flow NPDR eyes), we found they had a significantly different retinal vessel density compared to the remaining eyes in the respective groups. Retinal blood velocity and flow is increased in eyes with DM no DR, while these parameters are decreased in eyes with mild NPDR compared to healthy age-matched controls. The similarity of OCTA vessel density among outliers in the two diabetic groups suggests an initial increase followed by progressive decline in blood flow and OCTA vessel density with progression to clinical retinopathy, which warrants further investigation.
Potentials of Host-Directed Therapies in Tuberculosis Management
Authors: Dara Yash , Volcani Doron , Shah Kush , Shin Kevin , Venketaraman Vishwanath
Published: August 2019

Tuberculosis (TB) remains as a leading cause of mortality in developing countries, persisting as a major threat to the global public health. Current treatment involving a long antibiotic regimen brings concern to the topic of patient compliance, contributing to the emergence of drug resistant TB. The current review will provide an updated outlook on novel anti-TB therapies that can be given as adjunctive agents to current anti-TB treatments, with a particular focus on modulating the host immune response to effectively target all forms of TB. Additional potential therapeutic pathway targets, including lipid metabolism alteration and vascular endothelial growth factor (VEGF)-directed therapies, are discussed.
National Temporal Trend Analysis of Infective Endocarditis among Patients Infected with HIV in Spain (1997–2014): A Retrospective Study
Authors: Muñoz-Moreno Maria Fe , Ryan Pablo , Alvaro-Meca Alejandro , Valencia Jorge , Tamayo Eduardo , Resino Salvador
Published: August 2019

Background: People living with human immunodeficiency virus (HIV) (PLWH) form a vulnerable population for the onset of infective endocarditis (IE). We aimed to analyze the epidemiological trend of IE, as well as its microbiological characteristics, in PLWH during the combined antiretroviral therapy era in Spain. Methods: We performed a retrospective study (1997–2014) in PLWH with data obtained from the Spanish Minimum Basic Data Set. We selected 1800 hospital admissions with an IE diagnosis, which corresponded to 1439 patients. Results: We found significant downward trends in the periods 1997–1999 and 2008–2014 in the rate of hospital admissions with an IE diagnosis (from 21.8 to 3.8 events per 10,000 patients/year; p < 0.001), IE incidence (from 18.2 to 2.9 events per 10,000 patients/year; p < 0.001), and IE mortality (from 23.9 to 5.5 deaths per 100,000 patient-years; p < 0.001). The most frequent microorganisms involved were staphylococci (50%; 42.7% Staphylococcus aureus and 7.3% coagulase-negative staphylococci (CoNS)), followed by streptococci (9.3%), Gram-negative bacilli (8.3%), enterococci (3%), and fungus (1.4%). During the study period, we found a downward trend in the rates of CoNS (p < 0.001) and an upward trends in streptococci (p = 0.001), Gram-negative bacilli (p < 0.001), enterococci (p = 0.003), and fungus (p < 0.001) related to IE, mainly in 2008–2014. The rate of community-acquired IE showed a significant upward trend (p = 0.001), while the rate of health care-associated IE showed a significant downward trend (p < 0.001). Conclusions: The rates of hospital admissions, incidence, and mortality related to IE diagnosis in PLWH in Spain decreased from 1997 to 2014, while other changes in clinical characteristics, mode of acquisition, and pathogens occurred over this time.
The Combination of Patient-Reported Clinical Symptoms and an Endoscopic Score Correlates Well with Health-Related Quality of Life in Patients with Ulcerative Colitis
Authors: Aniwan Satimai , Bruining David H. , Park Sang Hyoung , Al-Bawardy Badr , Kane Sunanda V. , Coelho Prabhu Nayantara , Kisiel John B. , Raffals Laura E. , Papadakis Konstantinos A. , Pardi Darrell S. , Tremaine William J. , Loftus Edward V.
Published: August 2019

Background and aims: Patient-reported outcomes (PROs) will become increasingly important as primary endpoints in future clinical trials. We aimed to evaluate the relationship between health-related quality of life (HRQoL) and the combination of patient-reported clinical symptoms (ClinPRO2) and Mayo endoscopic subscore (MES) in patients with ulcerative colitis (UC). Methods: We conducted a prospective cross-sectional study of 90 consecutive UC patients who were scheduled for sigmoidoscopy or colonoscopy. All patients completed the following questionnaires: (1) self-rated rectal bleeding and stool frequency (ClinPRO2); (2) Short Inflammatory Bowel Disease Questionnaire (SIBDQ); (3) European Quality of Life 5-Dimensions 3-Level (EQ5D3L); (4) Work Productivity and Activity Impairment questionnaire (WPAI); (5) Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT-F); and (6) Hospital Anxiety and Depression Scale (HADS). The endoscopic images were graded according to the MES. “No symptoms” was defined as a symptom score of 0, and “mucosal healing” was defined as MES score of 0–1. Correlations between the combined ClinPRO2 and MES with HRQoL were assessed using Spearman’s correlation coefficients. Results: The combination of the ClinPRO2 and MES was well correlated to SIBDQ (r = −0.70), EQ5D3L (r = −0.51), WPAI (r = 0.62), FACIT-F (r = −0.58), and HADS-depression (r = 0.45). SIBDQ scores had strong correlations with FACIT-F (r = 0.86), WPAI (r = −0.80), and HADS-depression (r = −0.75) (p < 0.05 for all correlations). Patients with no symptoms reported the greatest all HRQoL scores. Conclusions: In patients with ulcerative colitis, the combination of a ClinPRO2 and the MES had good correlation with the SIBDQ. In addition, SIBDQ was well correlated to the various HRQoL.
Assessing Individual Performance in Team Sports: A New Method Developed in Youth Volleyball
Authors: Bisagno Elisa , Morra Sergio , Basciano Martina , Rosina Carola , Vitali Francesca
Published: August 2019

Studying the role of individual differences in team sports performance is a challenge. The main problem is having an available measure of individual performance of each member of the team. In particular, in youth sports, where the level of specialization is reactively low, it appears appropriate that this measure takes the entire performance of the athlete into consideration (i.e., that it assesses all of the athlete’s gestures), while maintaining an ecological validity criterion. Therefore, we devised and calculated an individual assessment measure in volleyball following the subsequent steps: Firstly, we video-recorded at least three volleyball games for each of the 114 youth volleyball players who participated in the study. Then, two independent expert observers evaluated each individual performance by attributing a score to every single gesture performed by the athletes during the games. The derived individual score was adjusted and controlled for the team performance measure, namely the result of each Set the athlete participated in (and for the amount of participation of the athlete to each game). The final measure of individual performance in volleyball proved to be reliable, showing a high level of interrater agreement (r = .841, p < .001) and a significant correlation with the amount of experience in volleyball (r = .173, p < .05).
A Land-Based and Spatial Assessment of Local Food Capacity in Northern Idaho, USA
Authors: Liao Felix Haifeng , Gordon Brendan , DePhelps Colette , Saul Darin , Fan Chao , Feng Wenlong
Published: August 2019

Across the United States, there has been a growing interest in local food production, which provides an alternative way to increase self-sufficiency and support greater well-being and food security at the community level. This study focused on the Northern Panhandle region of Idaho, where opportunities derived from the local food movement have emerged in several resort and college towns. This research integrated spatial analysis and modeling in a geographic information system (GIS) environment and a linear-programming (LP) optimization approach to identify, quantify, and map these potential opportunities. The obtained results show that existing local food producers are located in the urban fringe and on productive cropland. The foodshed model further suggests that Northern Idaho has enough farmland to feed its whole population within an average distance of 49 km or 31 miles. An alternative land use scenario was explored that involves removing marginal cropland with high soil erodibility from commodity cropping to improve the ecological benefits of local food production. The results of the study, including nuanced evidence of growing demand for local-food products, the existence of enough cropland capacity to meet demand, and potential environmental benefits, are quite encouraging to local food advocates in Northern Idaho and other areas and demonstrate the utility of land-based foodshed analysis.
An Overview on the Tribological Performance of Titanium Alloys with Surface Modifications for Biomedical Applications
Authors: Kaur Sukhpreet , Ghadirinejad Khashayar , H. Oskouei Reza
Published: August 2019

The need for metallic biomaterials will always remain high with their growing demand in joint replacement in the aging population. This creates need for the market and researchers to focus on the development and advancement of the biometals. Desirable characteristics such as excellent biocompatibility, high strength, comparable elastic modulus with bones, good corrosion resistance, and high wear resistance are the significant issues to address for medical implants, particularly load-bearing orthopedic implants. The widespread use of titanium alloys in biomedical implants create a big demand to identify and assess the behavior and performance of these alloys when used in the human body. Being the most commonly used metal alloy in the fabrication of medical implants, mainly because of its good biocompatibility and corrosion resistance together with its high strength to weight ratio, the tribological behavior of these alloys have always been an important subject for study. Titanium alloys with improved wear resistance will of course enhance the longevity of implants in the body. In this paper, tribological performance of titanium alloys (medical grades) is reviewed. Various methods of surface modifications employed for titanium alloys are also discussed in the context of wear behavior.
Effect of the Iron Reduction Index on the Mechanical and Chemical Properties of Continuous Basalt Fiber
Authors: Luo Lida , Zhang Qichang , Wang Qingwei , Xiao Jiwen , Liu Jin , Ding Linfeng , Jiang Weizhong
Published: August 2019

Basalt glass belongs to the iron-rich aluminosilicate glass system; thus, the iron content and the iron redox index (IRI=Fe2+/Fetotal) influence the viscosity, density, mechanical and chemical properties of basalt fiber (BF). In this work, continuous BFs with IRIs ranging from 0.21–0.87 were prepared by adding a different amount of redox agents. An economical and easily accessible testing method—the spectral photometric method with 1,10-phenanthroline—is applied to measure the IRI with convinced accuracy, which has been approved by Mössbauer spectra and X-ray fluorescence analysis. The tensile strength of the BF samples increases approximately linearly with increasing IRI as a function of σ = 227.9 IRI + 780.0 . The FT-IR results indicate that, with increasing IRI, the ferric ions are replaced by the much stronger network formers (Al3+ and Si4+), hence the increased the tensile strength. The X-ray diffraction results show an amorphous nature of BF samples. Moreover, the tensile strength is significantly decreased after the alkali corrosion, which is partly attributed to the severe surface damaging according to the SEM results. This work proved the feasibility of mechanical property improvement in BF production by controlling the iron redox index.
Internal Cracks and Non-Metallic Inclusions as Root Causes of Casting Failure in Sugar Mill Roller Shafts
Authors: Jamil Muhammad , Khan Aqib Mashood , Hegab Hussien , Sarfraz Shoaib , Sharma Neeraj , Mia Mozammel , Gupta Munish Kumar , Zhao GuLong , Moustabchir H. , Pruncu Catalin I.
Published: August 2019

The sugar mill roller shaft is one of the critical parts of the sugar industry. It requires careful manufacturing and testing in order to meet the stringent specification when it is used for applications under continuous fatigue and wear environments. For heavy industry, the manufacturing of such heavy parts (>600 mm diameter) is a challenge, owing to ease of occurrence of surface/subsurface cracks and inclusions that lead to the rejection of the final product. Therefore, the identification and continuous reduction of defects are inevitable tasks. If the defect activity is controlled, this offers the possibility to extend the component (sugar mill roller) life cycle and resistance to failure. The current study aims to explore the benefits of using ultrasonic testing (UT) to avoid the rejection of the shaft in heavy industry. This study performed a rigorous evaluation of defects through destructive and nondestructive quality checks in order to detect the causes and effects of rejection. The results gathered in this study depict macro-surface cracks and sub-surface microcracks. The results also found alumina and oxide type non-metallic inclusions, which led to surface/subsurface cracks and ultimately the rejection of the mill roller shaft. A root cause analysis (RCA) approach highlighted the refractory lining, the hot-top of the furnace and the ladle as significant causes of inclusions. The low-quality flux and refractory lining material of the furnace and the hot-top, which were possible causes of rejection, were replaced by standard materials with better quality, applied by their standardized procedure, to prevent this problem in future production. The feedback statistics, evaluated over more than one year, indicated that the rejection rate was reduced for defective production by up to 7.6%.