bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Uncertainty and Negative Emotion in Chinese Parents’ Self-Medication of Children with Antibiotics
Authors: Pei Di , Kreps Gary , Zhao Xiaoquan
Published: August 2023

Parents self-medicating their children with antibiotics (SMA) without consulting healthcare professionals is a common practice in China. Previous research has focused primarily on examining the socioeconomic factors that contribute to the prevalence of SMA. This study adopts and extends the theory of planned behavior to understand the cognitive and emotional factors that are associated with parental SMA in China. The responses to an online survey were collected from 961 parents of children aged 6–12 years old, primarily from Gansu, Shandong, and Shaanxi provinces. More than half of the participants (66.5%) engaged in parental SMA. Amoxicillin, Cephradine, and Azithromycin were the most frequently selected antibiotics used for children. Structural equation modeling showed that uncertainty was positively associated with negative emotions, which were in turn positively associated with attitude toward SMA. Uncertainty was also negatively associated with perceived behavioral control (PBC), but the association between PBC and SMA behavior was not significant. Attitude and subjective norm were both positively associated with SMA behavior. The relationship between subjective norm and attitude was also positive. Understanding the psychological factors driving parental SMA may inform tailored interventions to promote responsible antibiotic use among parents.
An Individualized Training Program for PE Teachers Based on Self-Determination Theory as a Way to Improve Students’ Psychosocial Health: A Study Protocol
Authors: González-Peño Alba , Franco Evelia , Martín-Hoz Laura , Coterón Javier
Published: August 2023

The interactions that take place in physical education (PE) between teachers and students have received large attention from the scientific community. However, despite the existence of different studies aiming to promote motivation among students through school interventions, there seem to be no interventions based on motivational strategies in which interventions are personalized to better fit teachers’ own characteristics on the basis of theoretical contents grounded in self-determination theory. This study aims to present a protocol intervention in the PE context based on SDT to improve teaching behaviours through an individualized and lifelong training program. This protocol is a convenience study in which PE teachers will design and implement motivational strategies to increase students’ motivation in class. The training program will take place along the intervention to allow teachers to personalize their implementation of motivational strategies according to their specific context. Data collection will be conducted before, during and after the intervention using recorded sessions (observational methodology), interviews (qualitative approach), and questionnaires (quantitative approach). The measures will assess teachers’ and students’ perceived teaching styles. This intervention program is expected to change and improve the quality of teaching behaviours, which could foster students’ psychosocial health.
Spatial Distribution Characteristics and Influencing Factors on the Retail Industry in the Central Urban Area of Lanzhou City at the Scale of Daily Living Circles
Authors: Lu Chenyu , Yu Changbin , Xin Yu , Zhang Wendi
Published: August 2023

Using a people-centered approach to new urbanization, China has committed to building high-quality living environments through improving urban livability and promoting a stronger sense of belonging among residents. Retail stores serve as one of the most immediate and accessible destinations for residents’ consumption, and their spatial configuration has a direct impact on residents’ satisfaction and happiness in their daily lives. In this context, for the present study we selected the central urban area of Lanzhou City as the case study area. Based on POI data and using the daily life circle as the basic unit, we applied methods such as kernel density analysis, hotspot analysis, and the Shannon–Weaver index to analyze spatial distribution patterns of the retail industry. Furthermore, we applied Geodetector to analyze the impacts of four factors that are closely related to the retail industry: economic level, convenience level, market demand, and location. The conclusions are as follows: In the central urban area of Lanzhou, the retail industry exhibits a belt distribution pattern along the Yellow River. The density of distribution gradually decreases from the city center toward the outskirts, forming four prominent agglomeration centers. Overall, within the central urban area of Lanzhou, the spatial distribution of the retail industry at the scale of daily living circles shows that only a small proportion of the industry demonstrates noticeable clustering effects. In terms of spatial patterns, the retail industry at the scale of the daily living circles demonstrates similar characteristics in terms of diversity and agglomeration distribution. It exhibits a decreasing trend from the urban core toward the peripheral areas. The agglomeration distribution pattern of the retail industry in the central urban area of Lanzhou is considerably influenced by market demand, economic level, convenience, and location. The spatial distribution of the retail industry in the central urban area is primarily influenced by economic factors and convenience, while market demand plays a major role and location has a relatively minimal impact.
Enhancing Indoor Air Quality Estimation: A Spatially Aware Interpolation Scheme
Authors: Jung Seungwoog , Han Seungwan , Choi Hoon
Published: August 2023

The comprehensive and accurate assessment of the indoor air quality (IAQ) in large spaces, such as offices or multipurpose facilities, is essential for IAQ management. It is widely recognized that various IAQ factors affect the well-being, health, and productivity of indoor occupants. In indoor environments, it is important to assess the IAQ in places where it is difficult to install sensors due to space constraints. Spatial interpolation is a technique that uses sample values of known points to predict the values of other unknown points. Unlike in outdoor environments, spatial interpolation is difficult in large indoor spaces due to various constraints, such as being separated into rooms by walls or having facilities such as air conditioners or heaters installed. Therefore, it is necessary to identify independent or related regions in indoor spaces and to utilize them for spatial interpolation. In this paper, we propose a spatial interpolation technique that groups points with similar characteristics in indoor spaces and utilizes the characteristics of these groups for spatial interpolation. We integrated the IAQ data collected from multiple locations within an office space and subsequently conducted a comparative experiment to assess the accuracy of our proposed method in comparison to commonly used approaches, such as inverse distance weighting (IDW), kriging, natural neighbor interpolation, and the radial basis function (RBF). Additionally, we performed experiments using the publicly available Intel Lab dataset. The experimental results demonstrate that our proposed scheme outperformed the existing methods. The experimental results show that the proposed method was able to obtain better predictions by reflecting the characteristics of regions with similar characteristics within the indoor space.
Evaluation of Solutions Containing Fluoride, Sodium Trimetaphosphate, Xylitol, and Erythritol, Alone or in Different Associations, on Dual-Species Biofilms
Authors: Zen Igor , Delbem Alberto Carlos Botazzo , Martins Tamires Passadori , de Morais Leonardo Antônio , Sampaio Caio , Hosida Thayse Yumi , Monteiro Douglas Roberto , Pessan Juliano Pelim
Published: August 2023

Although the association of polyols/polyphosphates/fluoride has been demonstrated to promote remarkable effects on dental enamel, little is known on their combined effects on biofilms. This study assessed the effects of solutions containing fluoride/sodium trimetaphosphate (TMP)/xylitol/erythritol on dual-species biofilms of Streptococcus mutans and Candida albicans. Biofilms were grown in the continuous presence of these actives alone or in different associations. Quantification of viable plate counts, metabolic activity, biofilm biomass, and extracellular matrix components were evaluated. Overall, fluoride and TMP were the main actives that significantly influenced most of the variables analyzed, with a synergistic effect between them for S. mutans CFUs, biofilm biomass, and protein content of the extracellular matrix (p < 0.05). A similar trend was observed for biofilm metabolic activity and carbohydrate concentrations of the extracellular matrix, although without statistical significance. Regarding the polyols, despite their modest effects on most of the parameters analyzed when administered alone, their co-administration with fluoride and TMP led to a greater reduction in S. mutans CFUs and biofilm biomass compared with fluoride alone at the same concentration. It can be concluded that fluoride and TMP act synergistically on important biofilm parameters, and their co-administration with xylitol/erythritol significantly impacts S. mutans CFUs and biomass reduction.
Updates on Larynx Cancer: Risk Factors and Oncogenesis
Authors: Liberale Carlotta , Soloperto Davide , Marchioni Alessandro , Monzani Daniele , Sacchetto Luca
Published: August 2023

Laryngeal cancer is a very common tumor in the upper aero-digestive tract. Understanding its biological mechanisms has garnered significant interest in recent years. The development of laryngeal squamous cell carcinoma (LSCC) follows a multistep process starting from precursor lesions in the epithelium. Various risk factors have been associated with laryngeal tumors, including smoking, alcohol consumption, opium use, as well as infections with HPV and EBV viruses, among others. Cancer development involves multiple steps, and genetic alterations play a crucial role. Tumor suppressor genes can be inactivated, and proto-oncogenes may become activated through mechanisms like deletions, point mutations, promoter methylation, and gene amplification. Epigenetic modifications, driven by miRNAs, have been proven to contribute to LSCC development. Despite advances in molecular medicine, there are still aspects of laryngeal cancer that remain poorly understood, and the underlying biological mechanisms have not been fully elucidated. In this narrative review, we examined the literature to analyze and summarize the main steps of carcinogenesis and the risk factors associated with laryngeal cancer.
Genomic Scan for Runs of Homozygosity and Selective Signature Analysis to Identify Candidate Genes in Large White Pigs
Authors: Yin Chang , Wang Yuwei , Zhou Peng , Shi Haoran , Ma Xinyu , Yin Zongjun , Liu Yang
Published: August 2023

Large White pigs are extensively utilized in China for their remarkable characteristics of rapid growth and the high proportion of lean meat. The economic traits of pigs, comprising reproductive and meat quality traits, play a vital role in swine production. In this study, 2295 individuals, representing three different genetic backgrounds Large White pig populations were used: 500 from the Canadian line, 295 from the Danish line, and 1500 from the American line. The GeneSeek 50K GGP porcine HD array was employed to genotype the three pig populations. Firstly, genomic selective signature regions were identified using the pairwise fixation index (FST) and locus-specific branch length (LSBL). By applying a top 1% threshold for both parameters, a total of 888 candidate selective windows were identified, harbouring 1571 genes. Secondly, the investigation of regions of homozygosity (ROH) was performed utilizing the PLINK software. In total, 25 genomic regions exhibiting a high frequency of ROHs were detected, leading to the identification of 1216 genes. Finally, the identified potential functional genes from candidate genomic regions were annotated, and several important candidate genes associated with reproductive traits (ADCYAP1, U2, U6, CETN1, Thoc1, Usp14, GREB1L, FGF12) and meat quality traits (MiR-133, PLEKHO1, LPIN2, SHANK2, FLVCR1, MYL4, SFRP1, miR-486, MYH3, STYX) were identified. The findings of this study provide valuable insights into the genetic basis of economic traits in Large White pigs and may have potential use in future pig breeding programs.
Construction and Validation of a Reliable Disulfidptosis-Related LncRNAs Signature of the Subtype, Prognostic, and Immune Landscape in Colon Cancer
Authors: Dong Xiaoqian , Liao Pan , Liu Xiaotong , Yang Zhenni , Wang Yali , Zhong Weilong , Wang Bangmao
Published: August 2023

Disulfidptosis, a novel form of regulated cell death (RCD) associated with metabolism, represents a promising intervention target in cancer therapy. While abnormal lncRNA expression is associated with colon cancer development, the prognostic potential and biological characteristics of disulfidptosis-related lncRNAs (DRLs) remain unclear. Consequently, the research aimed to discover a novel indication of DRLs with significant prognostic implications, and to investigate their possible molecular role in the advancement of colon cancer. Here, we acquired RNA-seq data, pertinent clinical data, and genomic mutations of colon adenocarcinoma (COAD) from the TCGA database, and then DRLs were determined through Pearson correlation analysis. A total of 434 COAD patients were divided in to three subgroups through clustering analysis based on DRLs. By utilizing univariate Cox regression, the least absolute shrinkage and selection operator (LASSO) algorithm, and multivariate Cox regression analysis, we ultimately created a prognostic model consisting of four DRLs (AC007728.3, AP003555.1, ATP2B1.AS1, and NSMCE1.DT), and an external database was used to validate the prognostic features of the risk model. According to the Kaplan–Meier curve analysis, patients in the low-risk group exhibited a considerably superior survival time in comparison to those in the high-risk group. Enrichment analysis revealed a significant association between metabolic processes and the genes that were differentially expressed in the high- and low-risk groups. Additionally, significant differences in the tumor immune microenvironment landscape were observed, specifically pertaining to immune cells, function, and checkpoints. High-risk patients exhibited a low likelihood of immune evasion, as indicated by the Tumor Immune Dysfunction and Exclusion (TIDE) analysis. Patients who exhibit both a high risk and high Tumor Mutational Burden (TMB) experience the least amount of time for survival, whereas those belonging to the low-risk and low-TMB category demonstrate the most favorable prognosis. In addition, the risk groups determined by the 4-DRLs signature displayed distinct drug sensitivities. Finally, we confirmed the levels of expression for four DRLs through rt-qPCR in both tissue samples from colon cancer patients and cell lines. Taken together, the first 4-DRLs-based signature we proposed may serve for a hopeful instrument for forecasting the prognosis, immune landscape, and therapeutic responses in colon cancer patients, thereby facilitating optimal clinical decision-making.
Porphyromonas gingivalis Peptidyl Arginine Deiminase (PPAD) in the Context of the Feed-Forward Loop of Inflammation in Periodontitis
Authors: Prucsi Zsombor , Zimny Agnieszka , Płonczyńska Alicja , Zubrzycka Natalia , Potempa Jan , Sochalska Maja
Published: August 2023

Periodontitis is a widespread chronic inflammatory disease caused by a changed dysbiotic oral microbiome. Although multiple species and risk factors are associated with periodontitis, Porphyromonas gingivalis has been identified as a keystone pathogen. The immune-modulatory function of P. gingivalis is well characterized, but the mechanism by which this bacterium secretes peptidyl arginine deiminase (PPAD), a protein/peptide citrullinating enzyme, thus contributing to the infinite feed-forward loop of inflammation, is not fully understood. To determine the functional role of citrullination in periodontitis, neutrophils were stimulated by P. gingivalis bearing wild-type PPAD and by a PPAD mutant strain lacking an active enzyme. Flow cytometry showed that PPAD contributed to prolonged neutrophil survival upon bacterial stimulation, accompanied by the secretion of aberrant IL-6 and TNF-α. To further assess the complex mechanism by which citrullination sustains a chronic inflammatory state, the ROS production and phagocytic activity of neutrophils were evaluated. Flow cytometry and colony formation assays showed that PPAD obstructs the resolution of inflammation by promoting neutrophil survival and the release of pro-inflammatory cytokines, while enhancing the resilience of the bacteria to phagocytosis.
Iron Load Toxicity in Medicine: From Molecular and Cellular Aspects to Clinical Implications
Authors: Kontoghiorghes George J.
Published: August 2023

Iron is essential for all organisms and cells. Diseases of iron imbalance affect billions of patients, including those with iron overload and other forms of iron toxicity. Excess iron load is an adverse prognostic factor for all diseases and can cause serious organ damage and fatalities following chronic red blood cell transfusions in patients of many conditions, including hemoglobinopathies, myelodyspasia, and hematopoietic stem cell transplantation. Similar toxicity of excess body iron load but at a slower rate of disease progression is found in idiopathic haemochromatosis patients. Excess iron deposition in different regions of the brain with suspected toxicity has been identified by MRI T2* and similar methods in many neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease. Based on its role as the major biological catalyst of free radical reactions and the Fenton reaction, iron has also been implicated in all diseases associated with free radical pathology and tissue damage. Furthermore, the recent discovery of ferroptosis, which is a cell death program based on free radical generation by iron and cell membrane lipid oxidation, sparked thousands of investigations and the association of iron with cardiac, kidney, liver, and many other diseases, including cancer and infections. The toxicity implications of iron in a labile, non-protein bound form and its complexes with dietary molecules such as vitamin C and drugs such as doxorubicin and other xenobiotic molecules in relation to carcinogenesis and other forms of toxicity are also discussed. In each case and form of iron toxicity, the mechanistic insights, diagnostic criteria, and molecular interactions are essential for the design of new and effective therapeutic interventions and of future targeted therapeutic strategies. In particular, this approach has been successful for the treatment of most iron loading conditions and especially for the transition of thalassemia from a fatal to a chronic disease due to new therapeutic protocols resulting in the complete elimination of iron overload and of iron toxicity.
P2X7 Receptor Regulates Collagen Expression in Human Intestinal Fibroblasts: Relevance in Intestinal Fibrosis
Authors: Lis-López Lluis , Bauset Cristina , Seco-Cervera Marta , Macias-Ceja Dulce , Navarro Francisco , Álvarez Ángeles , Esplugues Juan Vicente , Calatayud Sara , Barrachina Maria Dolores , Ortiz-Masià Dolores , Cosín-Roger Jesús
Published: August 2023

Intestinal fibrosis is a common complication that affects more than 50% of Crohn´s Disease (CD) patients. There is no pharmacological treatment against this complication, with surgery being the only option. Due to the unknown role of P2X7 in intestinal fibrosis, we aim to analyze the relevance of this receptor in CD complications. Surgical resections from CD and non-Inflammatory Bowel Disease (IBD) patients were obtained. Intestinal fibrosis was induced with two different murine models: heterotopic transplant model and chronic-DSS colitis in wild-type and P2X7-/- mice. Human small intestine fibroblasts (HSIFs) were transfected with an siRNA against P2X7 and treated with TGF-β. A gene and protein expression of P2X7 receptor was significantly increased in CD compared to non-IBD patients. The lack of P2X7 in mice provoked an enhanced collagen deposition and increased expression of several profibrotic markers in both murine models of intestinal fibrosis. Furthermore, P2X7-/- mice exhibited a higher expression of proinflammatory cytokines and a lower expression of M2 macrophage markers. Moreover, the transient silencing of the P2X7 receptor in HSIFs significantly induced the expression of Col1a1 and potentiated the expression of Col4 and Col5a1 after TGF-β treatment. P2X7 regulates collagen expression in human intestinal fibroblasts, while the lack of this receptor aggravates intestinal fibrosis.
Heterodimerization of Chemoreceptors TAS1R3 and mGlu2 in Human Blood Leukocytes
Authors: Ball Lena , Bauer Julia , Krautwurst Dietmar
Published: August 2023

The expression of canonical chemosensory receptors of the tongue, such as the heteromeric sweet taste (TAS1R2/TAS1R3) and umami taste (TAS1R1/TAS1R3) receptors, has been demonstrated in many extra-oral cells and tissues. Gene expression studies have revealed transcripts for all TAS1 and metabotropic glutamate (mGlu) receptors in different types of immune cells, where they are involved, for example, in the chemotaxis of human neutrophils and the protection of T cells from activation-induced cell death. Like other class-C G protein-coupling receptors (GPCRs), TAS1Rs and mGlu receptors form heteromers within their families. Since mGlu receptors and TAS1R1/TAS1R3 share the same ligand, monosodium glutamate (MSG), we hypothesized their hitherto unknown heteromerization across receptor families in leukocytes. Here we show, by means of immunocytochemistry and co-IP/Western analysis, that across class-C GPCR families, mGlu2 and TAS1R3 co-localize and heterodimerize in blood leukocytes. Expressing the recombinant receptors in HEK-293 cells, we validated their heterodimerization by bioluminescence resonance energy transfer. We demonstrate MSG-induced, mGlu2/TAS1R3 heteromer-dependent gain-of-function and pertussis toxin-sensitive signaling in luminescence assays. Notably, we show that mGlu2/TAS1R3 is necessary and sufficient for MSG-induced facilitation of N-formyl-methionyl-leucyl-phenylalanine-stimulated IL-8 secretion in neutrophils, using receptor-specific antagonists. In summary, our results demonstrate mGlu2/TAS1R3 heterodimerization in leukocytes, suggesting cellular function-tailored chemoreceptor combinations to modulate cellular immune responses.
Early Molecular Immune Responses of Turbot (Scophthalmus maximus L.) Following Infection with Aeromonas salmonicida subsp. salmonicida
Authors: Fajardo Carlos , Santos Paulo , Passos Ricardo , Vaz Mariana , Azeredo Rita , Machado Marina , Fernández-Boo Sergio , Baptista Teresa , Costas Benjamin
Published: August 2023

Turbot aquaculture production is an important economic activity in several countries around the world; nonetheless, the incidence of diseases, such furunculosis, caused by the etiological agent A. salmonicida subsp. salmonicida, is responsible for important losses to this industry worldwide. Given this perspective, this study aimed to evaluate early immune responses in turbot (S. maximus L.) following infection with A. salmonicida subsp. salmonicida. For this, 72 fish were individually weighed and randomly distributed into 6 tanks in a circulating seawater system. For the bacterial challenge, half of the individuals (3 tanks with 36 individuals) were infected using a peritoneal injection with the bacterial suspension, while the other half of individuals were injected with PBS and kept as a control group. Several factors linked to the innate immune response were studied, including not only haematological (white blood cells, red blood cells, haematocrit, haemoglobin, mean corpuscular volume, mean cell haemoglobin, mean corpuscular haemoglobin concentration, neutrophils, monocytes, lymphocytes, thrombocytes) and oxidative stress parameters, but also the analyses of the expression of 13 key immune-related genes (tnf-α, il-1β, il-8, pparα-1, acox1, tgf-β1, nf-kB p65, srebp-1, il-10, c3, cpt1a, pcna, il-22). No significant differences were recorded in blood or innate humoral parameters (lysozyme, anti-protease, peroxidase) at the selected sampling points. There was neither any evidence of significant changes in the activity levels of the oxidative stress indicators (catalase, glutathione S-transferase, lipid peroxidation, superoxide dismutase). In contrast, pro-inflammatory (tnf-α, il-1β), anti-inflammatory (il-10), and innate immune-related genes (c3) were up-regulated, while another gene linked with the lipid metabolism (acox1) was down-regulated. The results showed new insights about early responses of turbot following infection with A. salmonicida subsp. salmonicida.
Principles for External Human–Machine Interfaces
Authors: Wilbrink Marc , Cieler Stephan , Weiß Sebastian L. , Beggiato Matthias , Joisten Philip , Feierle Alexander , Oehl Michael
Published: August 2023

Automated vehicles will soon be integrated into our current traffic system. This development will lead to a novel mixed-traffic environment where connected and automated vehicles (CAVs) will have to interact with other road users (ORU). To enable this interaction, external human–machine interfaces (eHMIs) have been shown to have major benefits regarding the trust and acceptance of CAVs in multiple studies. However, a harmonization of eHMI signals seems to be necessary since the developed signals are extremely varied and sometimes even contradict each other. Therefore, the present paper proposes guidelines for designing eHMI signals, taking into account important factors such as how and in which situations a CAV needs to communicate with ORU. The authors propose 17 heuristics, the so-called eHMI-principles, as requirements for the safe and efficient use of eHMIs in a systematic and application-oriented manner.
Analysis of Factors Associated with Highway Personal Car and Truck Run-Off-Road Crashes: Decision Tree and Mixed Logit Model with Heterogeneity in Means and Variances Approaches
Authors: Champahom Thanapong , Wisutwattanasak Panuwat , Se Chamroeun , Banyong Chinnakrit , Jomnonkwao Sajjakaj , Ratanavaraha Vatanavongs
Published: August 2023

Among several approaches to analyzing crash research, the use of machine learning and econometric analysis has found potential in the analysis. This study aims to empirically examine factors influencing the single-vehicle crash for personal cars and trucks using decision trees (DT) and mixed binary logit with heterogeneity in means and variances (RPBLHMV) and compare model accuracy. The data in this study were obtained from the Department of Highway during 2011–2017, and the results indicated that the RPBLHMV was superior due to its higher overall prediction accuracy, sensitivity, and specificity values when compared to the DT model. According to the RPBLHMV results, car models showed that injury severity was associated with driver gender, seat belt, mount the island, defect equipment, and safety equipment. For the truck model, it was found that crashes located at intersections or medians, mounts on the island, and safety equipment have a significant influence on injury severity. DT results also showed that running off-road and hitting safety equipment can reduce the risk of death for car and truck drivers. This finding can illustrate the difference causing the dependent variable in each model. The RPBLHMV showed the ability to capture random parameters and unobserved heterogeneity. But DT can be easily used to provide variable importance and show which factor has the most significance by sequencing. Each model has advantages and disadvantages. The study findings can give relevant authorities choices for measures and policy improvement based on two analysis methods in accordance with their policy design. Therefore, whether advocating road safety or improving policy measures, the use of appropriate methods can increase operational efficiency.
Correction: Tian et al. Fluorescence Resonance Energy Transfer Properties and Auger Recombination Suppression in Supraparticles Self-Assembled from Colloidal Quantum Dots. Inorganics 2023, 11, 218
Authors: Tian Xinhua , Chang Hao , Dong Hongxing , Zhang Chi , Zhang Long
Published: August 2023

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Significantly Enhanced Self-Cleaning Capability in Anatase TiO2 for the Bleaching of Organic Dyes and Glazes
Authors: Zhao Tiangui , Cao Tihao , Bao Qifu , Dong Weixia , Li Ping , Gu Xingyong , Liang Yunzi , Zhou Jianer
Published: August 2023

In this study, the Mg2+-doped anatase TiO2 phase was synthesized via the solvothermal method by changing the ratio of deionized water and absolute ethanol Vwater/Vethanol). This enhances the bleaching efficiency under visible light. The crystal structure, morphology, and photocatalytic properties of Mg-doped TiO2 were characterized by X-ray diffraction, scanning electron microscopy, high-resolution transmission electron microscopy, N2 adsorption-desorption, UV-Vis spectroscopy analysis, etc. Results showed that the photocatalytic activity of the Mg2+-doped TiO2 sample was effectively improved, and the morphology, specific surface area, and porosity of TiO2 could be controlled by Vwater/Vethanol. Compared with the Mg-undoped TiO2 sample, Mg-doped TiO2 samples have higher photocatalytic properties due to pure anatase phase formation. The Mg-doped TiO2 sample was synthesized at Vwater/Vethanol of 12.5:2.5, which has the highest bleaching rate of 99.5% for the rhodamine B dye during 80 min under visible light. Adding Mg2+-doped TiO2 into the phase-separated glaze is an essential factor for enhancing the self-cleaning capability. The glaze samples fired at 1180 °C achieved a water contact angle of 5.623° at room temperature and had high stain resistance (the blot floats as a whole after meeting the water).
A Deep-Learning Approach to Spleen Volume Estimation in Patients with Gaucher Disease
Authors: Azuri Ido , Wattad Ameer , Peri-Hanania Keren , Kashti Tamar , Rosen Ronnie , Caspi Yaron , Istaiti Majdolen , Wattad Makram , Applbaum Yaakov , Zimran Ari , Revel-Vilk Shoshana , C. Eldar Yonina
Published: August 2023

The enlargement of the liver and spleen (hepatosplenomegaly) is a common manifestation of Gaucher disease (GD). An accurate estimation of the liver and spleen volumes in patients with GD, using imaging tools such as magnetic resonance imaging (MRI), is crucial for the baseline assessment and monitoring of the response to treatment. A commonly used method in clinical practice to estimate the spleen volume is the employment of a formula that uses the measurements of the craniocaudal length, diameter, and thickness of the spleen in MRI. However, the inaccuracy of this formula is significant, which, in turn, emphasizes the need for a more precise and reliable alternative. To this end, we employed deep-learning techniques, to achieve a more accurate spleen segmentation and, subsequently, calculate the resulting spleen volume with higher accuracy on a testing set cohort of 20 patients with GD. Our results indicate that the mean error obtained using the deep-learning approach to spleen volume estimation is 3.6 ± 2.7%, which is significantly lower than the common formula approach, which resulted in a mean error of 13.9 ± 9.6%. These findings suggest that the integration of deep-learning methods into the clinical routine practice for spleen volume calculation could lead to improved diagnostic and monitoring outcomes.
Association between Urinary AGEs and Circulating miRNAs in Children and Adolescents with Overweight and Obesity from the Italian I.Family Cohort: A Pilot Study
Authors: Russo Paola , Lauria Fabio , Sirangelo Ivana , Siani Alfonso , Iacomino Giuseppe
Published: August 2023

Modern dietary habits are linked to high exposure to Advanced Glycation End products (AGEs) mainly due to the dramatic increase in the consumption of highly processed foods in recent years. Body levels of these compounds vary with food intake and are almost interconnected with age and health status, formally embodying indicators of oxidative stress and inflammation in adults. However, the relationship between AGEs and health issues has not been definitively understood in children, and several pediatric investigations have produced conflicting evidence. Besides, despite extensive research, there are no universally accepted analytical techniques for measuring AGE levels in the human body, with several approaches available, each with its advantages and disadvantages. This pilot study aimed to investigate the association between urinary AGEs, measured using spectrofluorimetry-based assays, and circulating microRNAs (c-miRNAs) in a subsample (n = 22) of Italian children participating in the I.Family Study. Anthropometric measurements, biochemical markers, and miRNA profiles were assessed. The first indication of a relationship between urinary AGEs and c-miRNAs in the context of obesity was found. Specifically, four miRNAs, hsa-miR-10b-5p, hsa-miR-501-5p, hsa-miR-874-3p, and hsa-miR-2355-5p were significantly associated with levels of urinary AGEs. The association between AGEs, obesity, inflammation markers, and specific miRNAs highlights the complex interplay between these factors and their potential impact on cellular and tissue homeostasis. The discovery of altered c-miRNAs profiling has the potential to offer innovative methods for assessing early changes in the body’s AGE pool and allow recognition of an increased risk of disease susceptibility, routinely undetected until metabolic complications are identified.
Efficacy of Systematic Early-Second-Trimester Ultrasound Screening for Facial Anomalies: A Comparison between Prenatal Ultrasound and Postmortem Findings
Authors: Lamanna Bruno , Dellino Miriam , Cascardi Eliano , Rooke-Ley Mia , Vinciguerra Marina , Cazzato Gerardo , Malvasi Antonio , Vitagliano Amerigo , Nicolì Pierpaolo , Di Cosola Michele , Ballini Andrea , Cicinelli Ettore , Vimercati Antonella
Published: August 2023

Second-trimester 2D ultrasound (US) assessment of the fetal anatomy, as proposed by worldwide guidelines, allows detecting the majority of fetal malformation. However, the detection rates of fetal facial anomalies seem to still be low, mostly in cases of isolated facial malformation. The purpose of this research was to assess and analyze the concordance between the antenatal imaging findings from second-trimester US screening and the results of fetal postmortem autopsy. Between January 2010 and January 2020, there were 43 cases where fetuses with prenatal ultrasound diagnosis of a face abnormality, associated or not with a genetic syndrome or chromosomal disorder, following intrauterine death (IUD) or termination of pregnancy (TOP) after the 13 weeks of pregnancy, underwent autopsy in the Pathological Anatomy section of Bari Polyclinic specializing in feto-placental autopsies. The diagnosis of the fetal facial defects at ultrasound was compared with the findings at autopsy in all cases. A very high level of agreement between prenatal ultrasound and autopsy findings was found for facial abnormalities associated with genetic syndromes or numerical abnormality of chromosomes. A lower level of concordance was instead found in isolated facial defects or those associated with other organ anomalies, but not associated with genetic syndrome or numerical chromosome anomaly. A detailed examination of aborted fetuses led to successful quality control of early-second-trimester ultrasound detection of facial anomalies; however, it was less accurate for the isolated ones. It is, thus, reasonable to propose a systematic early-second-trimester prenatal ultrasound screening for facial anatomy by operators specialized in fetal medicine field, using 2D, 3D, and 4D techniques (two-, three-, and four-dimensional ultrasound).
Tuberculosis-Associated Hemophagocytic Lymphohistiocytosis: A Review of Current Literature
Authors: Fauchald Trym , Blomberg Bjørn , Reikvam Håkon
Published: August 2023

Hemophagocytic lymphohistiocytosis (HLH) is a condition of immune dysregulation and hyperinflammation, leading to organ failure and death. Malignancy, autoimmune conditions, and infections, including Mycobacterium tuberculosis (TB), are all considered triggers of HLH. The aim of this study was to review all reported cases of TB-associated HLH in English literature, and to summarize the epidemiology, diagnostics, treatment, and mortality in patients with concomitant HLH and TB. A systematic review of described cases with TB-associated HLH, via a structured literature search in the medical database PubMed, is presented. Additional articles were included through cross-referencing with existing review articles. Articles were reviewed based on a predetermined set of criteria. A total of 116 patients with TB-associated HLH were identified with a male:female ratio of about 3:2. The age at presentation ranged from 12 days to 83 years. Malignancy, autoimmunity, and renal failure were the most common comorbid conditions. Most patients received both tuberculostatic and specific immunomodulating treatment, which was associated with a 66% (48/73) survival rate compared to 56% (15/27) in those receiving only tuberculostatic treatment, and 0% (0/13) in those receiving only immunomodulating treatment. The survival rate was 55% overall. The overlapping presentation between disseminated TB and HLH poses challenging diagnostics and may delay diagnosis and treatment, leading to increased mortality. TB should be considered as a potential trigger of HLH; clinicians’ knowledge and awareness of this may result in the appropriate investigations needed to ensure diagnosis and proper treatment.
Understanding the Impact of Chronic Rhinosinusitis with Nasal Polyposis on Smell and Taste: An International Patient Experience Survey
Authors: Luke Louis , Lee Liam , Gokani Shyam Ajay , Boak Duncan , Boardman Jim , Philpott Carl
Published: August 2023

The aim is to understand the patient experience of living with chronic rhinosinusitis with nasal polyposis (CRSwNP), clinician interactions and how symptoms, smell and taste disturbance are managed. An anonymized, online survey was distributed through a UK charity, Fifth Sense, a UK otolaryngology clinic and online support groups to capture qualitative and quantitative data. Data were collected from 1st December 2022 to 1st February 2023. A total of 124 individuals participated. The majority were female (66%) and in the age range of 41–70 years; 74.2% of participants were from the UK with the rest from North America, Europe and Asia. A total of 107 participants declared they had CRSwNP. Rhinologists and general otolaryngology clinicians scored the highest for patient satisfaction whilst general practitioners scored the lowest. Satisfaction with the management of smell and taste disturbance was lower amongst all clinicians compared to overall satisfaction. Ratings correlated with response to therapy and clinician interactions. Respondents reported hyposmia/anosmia to be the most debilitating symptom. Surgery and oral steroids were considered to be effective; however, the benefit lasted less than six months (62%). Hyposmia/anosmia is a key CRSwNP symptom that has limited treatment options and is frequently undervalued by clinicians. There is a need for more effective management options, education and patient support.
Is the Combination of Plain X-ray and Probe-to-Bone Test Useful for Diagnosing Diabetic Foot Osteomyelitis? A Systematic Review and Meta-Analysis
Authors: Calvo-Wright María del Mar , Álvaro-Afonso Francisco Javier , López-Moral Mateo , García-Álvarez Yolanda , García-Morales Esther , Lázaro-Martínez José Luis
Published: August 2023

A systematic review and meta-analysis was conducted to assess the diagnostic accuracy of the combination of plain X-ray and probe-to-bone (PTB) test for diagnosing diabetic foot osteomyelitis (DFO). This systematic review has been registered in PROSPERO (a prospective international register of systematic reviews; identification code CRD42023436757). A literature search was conducted for each test separately along with a third search for their combination. A total of 18 articles were found and divided into three groups for separate analysis and comparison. All selected studies were evaluated using STROBE guidelines to assess the quality of reporting for observational studies. Meta-DiSc software was used to analyze the collected data. Concerning the diagnostic accuracy variables for each case, the pooled sensitivity (SEN) was higher for the combination of PTB and plain X-ray [0.94 (PTB + X-ray) vs. 0.91 (PTB) vs. 0.76 (X-ray)], as was the diagnostic odds ratio (DOR) (82.212 (PTB + X-ray) vs. 57.444 (PTB) vs. 4.897 (X-ray)). The specificity (SPE) and positive likelihood ratio (LR+) were equally satisfactory for the diagnostic combination but somewhat lower than for PTB alone (SPE: 0.83 (PTB + X-ray) vs. 0.86 (PTB) vs. 0.76 (X-ray); LR+: 5.684 (PTB + X-ray) vs. 6.344 (PTB) vs. 1.969 (X-ray)). The combination of PTB and plain X-ray showed high diagnostic accuracy comparable to that of MRI and histopathology diagnosis (the gold standard), so it could be considered useful for the diagnosis of DFO. In addition, this diagnostic combination is accessible and inexpensive but requires training and experience to correctly interpret the results. Therefore, recommendations for this technique should be included in the context of specialized units with a high prevalence of DFO.
Burden of Disease; the Current Status of the Diagnosis and Management of Atopic Dermatitis in China
Authors: Gu Chaoying , Yao Xu , Li Wei
Published: August 2023

Atopic dermatitis (AD) is now a global health problem and has been attracting extensive attention from both academic and public society in China. This review aimed to present the current status of the prevalence, disease burden, clinical features, diagnosis, and management of AD in China. The prevalence of AD has been increasing rapidly in China during the past decades, partially due to the increased recognition of the disease; there are still substantial amounts of over-diagnosed eczema and under-diagnosed AD. Chinese dermatologists see many AD patients with atypical manifestation, which poses a challenge to the diagnosis. The Chinese diagnostic criteria for adults and pediatric patients with AD have been proposed respectively and validated with high sensitivity and specificity. International and Chinese guidelines for management of AD have been popularized; however, there are still many practices that need verification through randomized case-control study. Dupilumab and JAK inhibitors have demonstrated favorable efficacy for AD patients in China, and a multidimensional approach is needed for selection of the patients and evaluation of the efficacy and safety. Patient education and long-term management for AD are just beginning in China, and need to be strengthened in the future.
The Relevance of Implementing the Systematic Screening of Perioperative Myocardial Injury in Noncardiac Surgery Patients
Authors: Popova Ekaterine , Paniagua-Iglesias Pilar , Álvarez-García Jesús , Vives-Borrás Miquel , González-Osuna Aránzazu , García-Osuna Álvaro , Rivas-Lasarte Mercedes , Hermenegildo-Chavez Gisela , Diaz-Jover Ruben , Azparren-Cabezon Gonzalo , Barceló-Trias Montserrat , Moustafa Abdel-Hakim , Aguilar-Lopez Raul , Ordonez-Llanos Jordi , Alonso-Coello Pablo
Published: August 2023

Perioperative myocardial injury (PMI) is a common cardiac complication. Recent guidelines recommend its systematic screening using high-sensitivity cardiac troponin (hs-cTn). However, there is limited evidence of local screening programs. We conducted a prospective, single-center study aimed at assessing the feasibility and outcomes of implementing systematic PMI screening. Hs-cTn concentrations were measured before and after surgery. PMI was defined as a postoperative hs-cTnT of ≥14 ng/L, exceeding the preoperative value by 50%. All patients were followed-up during the hospitalization, at one month and one year after surgery. The primary outcome was the incidence of death and major cardiovascular and cerebrovascular events (MACCE). The secondary outcomes focused on the individual components of MACCE. We included two-thirds of all eligible high-risk patients and achieved almost complete compliance with follow-ups. The prevalence of PMI was 15.7%, suggesting a higher presence of cardiovascular (CV) antecedents, increased perioperative CV complications, and higher preoperative hs-cTnT values. The all-cause death rate was 1.7% in the first month, increasing up to 11.2% at one year. The incidence of MACCE was 9.5% and 8.6% at the same time points. Given the observed elevated frequencies of PMI and MACCE, implementing systematic PMI screening is recommendable, particularly in patients with increased cardiovascular risk. However, it is important to acknowledge that achieving optimal screening implementation comes with various challenges and complexities.
Real-Life Performance and Clinical Outcomes of Portico Transcatheter Aortic Valve with FlexNav Delivery System: One-Year Data from a Single-Center Experience
Authors: Yildirim Abdullah , Genc Omer , Pacaci Emre , Sen Omer , Kurt Ibrahim Halil
Published: August 2023

Significant progress has been made in both valves and delivery systems (DSs) for transcatheter aortic valve replacement (TAVR) procedures. We aimed to present one-year real-life data regarding TAVR procedures using Portico transcatheter heart valves (THVs) with new-generation, low-profile FlexNav DSs. This retrospective, single-center study enrolled 169 consecutive patients (mean age: 75.8 years, 68% females) with severe aortic stenosis undergoing TAVR with Portico THVs and FlexNav DSs between 2020 and 2021. We evaluated safety and efficacy outcomes, following the VARC-3 consensus, periprocedurally and at 30 days and 1 year. Procedural success was observed in 95.9% of cases, and no procedural mortality occurred. At 30 days, the rates of all-cause mortality, cardiovascular mortality, and neurological events were 4.7%, 3.6%, and 3.0%, respectively. Additionally, major vascular complications, acute kidney injury, and bleeding were recorded at rates of 11.2%, 14.8%, and 7.7%, respectively. The 1-year data showed all-cause mortality, cardiovascular mortality, and neurological event rates of 10.7%, 8.3%, and 7.7%, respectively. The moderate paravalvular leak and permanent pacemaker rates at 1 year were 2.6% and 12.2%, respectively. This real-life data provided evidence of positive outcomes and high technical success with Portico THVs and FlexNav DSs. Furthermore, we found low rates of mortality and neurological events, with satisfactory hemodynamic and functional results.