bluuebunny/crossref_metadata_2025 huggingface dataset:
FAKTOR-FAKTOR YANG BEHUBUNGAN DENGAN BURNOUT AKADEMIK PADA MAHASISWA KEDOKTERAN
Authors: Wahab Revalita , Tjhin Purnamawati , Istriana Erita
Published: December 2024

ABSTRAK
Perubahan proses pembelajaran pada saat pandemi Covid-19 menyebabkan mahasiswa harus beradaptasi pada seluruh aspek pembelajaran dan dibutuhkan adaptasi mahasiswa yang bila tidak dilakukan dapat terjadi burnout yang berakibat pada penurunan pencapaian akademik. Tujuan penelitian ini untuk melihat hubungan faktor yang mempengaruhi burnout seperti usia, jenis kelamin, dan indeks prestasi akademik dengan burnout akademik. Desain penelitian cross-sectional  dilakukan pada 104 mahasiswa Fakultas Kedokteran Trisakti tahun kedua. School Burn'out Inventory versi Bahasa Indonesia yang telah divalidasi digunakan untuk mengukur burnout dan  analisis data uji Chi-Square digunakan untuk melihat melihat hubungan usia, jenis kelamin, serta indeks prestasi akademik burnout akademik dengan nilai kemaknaan 95%. Hasil penellitian ididapatkan mahasiswa burnout ringan 62,5%, 4,8% tidak mengalami burnout, 32,7%. burnout sedang - berat . Usia, jenis kelamin, dan indeks prestasi akademik tidak merupakan faktor yang berhubungan dengan burnout akademik. Simpulan perlu diindentifikasi faktor internal ataupun eksternal mahasiswa yang dapat menyebabkan burnout dengan pencapaian akademik.
Abstract
Due to pandemic Covid-19 earning process has changed and students had to adapt with massive rise of online learning. If the students could not adapt to the academic process, it will them to the a decrease in academic achievement. The factors related to academic burnout that need to identified. The aim of this study to investigate the  related factors cause of academic burnout in medical education students. The cross sectional study was conducted to 104 students, second year of medical student, of Universitas Trisakti using the School Burn’out inventory-Indonesian version to assess burnout. Data analysis was performed using Chi-suquare test. The results of this research  62,5% experienced mild burnout and only 4,8% students did not have burnout. Meanwhile 32,7% of the experienced moderate to severe burnout. Age, gender, and academic achievement there was no no significant relationship with academic burnout. The conclusion of this research is there was no significant relationship between age, gender, academic achievement with academic burnout. It is need identify the internal and external factor related burnout with academic achievement.
HUBUNGAN KADAR LEUKOSIT DAN TOTAL LIMFOSIT DENGAN KELAINAN PARU PADA PASIEN COVID-19
Authors: Putra Andika Fauzan , Khairani Rita
Published: December 2024

The impact of COVID-19 infection on the lungs is very broad. This lung organ disorder will affect the prognosis and also the patient’s quality of life. Markers are needed to identify the patient’s condition or patient’s prognosis so that they can estimate the patient’s condition from the start and determine further treatment and abnormalities that can be caused by the infection, especially in the patient’s lung organs. This study aims to reduce the incidence of post-COVID-19 pulmonary fibrosis by assessing the relationship between leukocyte levels and total lymphocyte count with lung abnormalities in COVID-19 patients. This research is an analytical study with a cross-sectional design using secondary data in the form of medical records from COVID-19 patients at the Budhi Asih Teaching Hospital, East Jakarta, with a total of 112 subjects. The data taken were leukocyte levels and total lymphocyte count based on laboratory results, and information on the patient’s pulmonary abnormalities documented in the medical record. The data were then analyzed by Chi-square test. The significance value used is p<0.05. Pneumonic lung abnormalities were dominated by increased leukocyte levels in 62 people (91.2%) and decreased total lymphocyte count in 63 people (94.1%). Leukocyte levels have a significant correlation with lung disorders (p = 0.021). The same thing was also shown by the total lymphocyte count which had a significant relationship with lung disorders (p = 0.001). There is a significant correlation between leukocyte levels and total lymphocyte count with lung abnormalities in COVID-19 patients.
CHLORINE RESIDUALS IN WASTEWATER TREATMENT: IMPLICATIONS FOR PATHOGEN INACTIVATION AND ENVIRONMENTAL IMPACT
Authors: Paramerta Ni Made Amanda Putri Paramerta , Nadhifah Dinda Fairuz , Marques Madalena G.J.V. , Saefurrahman Farhan , Stephanie Inna , Wikaningrum Temmy
Published: December 2024

Chlorine residuals are a critical component of wastewater treatment systems. Inactivation of pathogens is a crucial goal to protect public health and stop the spread of waterborne illnesses. The purpose of this study is to find out more about the use of chlorination to dominate all pathogenic bacteria in a safe environment. The method used in this paper is an approach to literature review, which is carried out by publishing articles, including browsing the internet, Google Scholar, and related journals. The study highlights the importance of understanding the relationship between the chlorine doses given and the resulting concentration of the residuals. In addition, the study emphasizes the potential consequences of an ecosystem, such as excessive algae growth and the contribution of methane gas production to greenhouse effects. Our findings focus on the need for a comprehensive approach to managing chlorine residuals, considering aspects of health in humans, effective inactivation that is pathogenic, and preserving the environment for long-term sustainability.
PENILAIAN RISIKO TEKNOLOGI INFORMASI PADA SOFTWARE HOUSEMENGGUNAKAN METODE FMEA DAN KONTROL DARI FRAMEWORK ISO27001:2013
Authors: Gita Azam , Putra W. Bramada , Zuhri M. Saifuddin , Abimanyu Farhan , Tifara Shelma , Puspitaningrum Ari Cahaya , Fitrani Laqma Dica
Published: December 2024

This study aims to identify and analyze the risks faced by CV Trisula Medika Teknologi, a software house company in the health sector. The method used is Failure Mode and Effects Analysis (FMEA) to identify potential failures and the Risk Priority Number (RPN) to determine the priority of risk handling. The results showed that there were 14 potential failures identified. Three of them have high-priority risks: hardware component damage, software update failure, and source code bugs. Damage to hardware components can cause the system to stop and disrupt the company's business processes. Failure of software updates can make the system vulnerable to malware and virus attacks. The existence of bugs in the source code can cause the system to be unstable and potentially result in fatal errors. The conclusion of this research emphasizes the importance of conducting regular risk identification and analysis to manage risks effectively and efficiently. By identifying and analyzing risks regularly, companies can take appropriate preventive measures to minimize the impact of risks. This can help the company improve the quality of its products and services, and increase customer confidence.
The attractiveness of the city of Barnaul in the assessments of students
Authors: Akhmedova Angelina Rustamovna , Zheldakova Arina Vladimirovna , Kolegaeva Elizaveta Aleksandrovna , Maslov Vladislav Sergeevich , Perin Sergei Aleksandrovich , Klimova Anastasiya  Alekseevna
Published: December 2024

The relevance of studying the problem of the attractiveness of the city in the assessments of student youth lies in the need to understand the factors influencing the choice of place of residence and study of the younger generation. In the context of globalization and migration processes, it is important to identify which aspects of the urban environment, infrastructure and social life attract students, as well as what problems they see in their city. Today, the phenomenon of social attractiveness is becoming more and more significant, as individuals begin to form a certain social rating in the system of any interactions, which affects their choice of moving, shopping, etc. For students, the assessment of attractiveness, as a rule, is of particular importance, since many medium-sized cities during the academic year are literally filled with students seeking to get an education or to gain a solid foothold and develop in this area. The study was implemented through a combination of two data collection methods – a mass survey and a focus group. The sample consisted of students from various higher educational institutions of the city of Barnaul (n=212). The sample is based on a system of cluster selection of respondents. The prevailing part of the student youth believes that the assessment of the attractiveness of the city of Barnaul is above average, due to its high geographical attractiveness, combining the advantages of forest-steppe and foothill zones. Social, tourism and innovation components have medium attractiveness, while economic attractiveness is low due to limited career opportunities. However, with efforts at the regional level, it is possible to increase the attractiveness of the city and prevent migration outflow. The overall assessment of the attractiveness of the city of Barnaul is at an above average level. This assessment is predetermined by the fact that there is a high degree of attractiveness within the geographical component. Barnaul combines all the advantages of the forest-steppe zone and the foothill zone, and the combination of these zones makes it possible to offset the disadvantages of aridity as the main criterion of the forest-steppe zone, as well as sharp temperature drops and recurrent frosts.
Marginalization of urban space: Experience of sociological research on the example of Khabarovsk
Authors: Turkulets Svetlana Evgenievna , Gareeva Irina Anatol'evna , Slesarev Aleksandr Valerievich , Garnaga Anastasiya Filippovna
Published: December 2024

The object of this study is the process of marginalization. The subject is the marginalization of urban space. The aim of the paper is to actualize and clarify the essence of the process of urban space marginalization, as well as to identify the features of urban space marginalization using the example of Khabarovsk. The article employs methods of scientific-theoretical analysis, generalization, and systematization of domestic and foreign research on marginalization problems, as well as empirical methods such as surveys, expert interviews, and the method of vernacular zoning. The theoretical part of the article demonstrates a multitude of different approaches to defining marginality and marginalization, on the one hand, and the lack of stable definitions of these concepts, on the other. This is primarily explained by the lack of a comprehensive approach to the study of the marginalization process. To provide a more complete definition of the process of urban space marginalization, the article applies an algorithm: causes – essence – implementation – consequences. The practical significance of studying the marginalization of urban spaces is determined by the need to control marginal areas in terms of the spread of various social deviations, as well as the need to forecast marginalization trends. Based on empirical studies of urban space (using the example of Khabarovsk), the study of public and expert opinions, and the use of the method of vernacular zoning, the overall "well-being" of different vernacular districts of the city was determined, the level of income of city residents living in these districts was assessed, the correlation between the determination of the level of "well-being" and the crime rate of the district was revealed, as well as the features of the stigmatization of urban spaces, which is an integral part of the process of marginalization.
The problem of periodization of the Russian cooperative movement of the late XIX – early XX centuries in the works of historians of the 2000s.
Authors: Bolotova Elena
Published: December 2024

Based on the historiographical analysis of the studies of the 2000s devoted to the history of various aspects of the Russian cooperative movement of the pre-revolutionary period, the authors' approaches to determining the stages in the development of the movement and their key characteristics are considered. In the 2000s, more than 500 researches were published, revealing both all-Russian trends and regional peculiarities in the development of cooperation in the late XIX – early XX centuries. Based on the achievements of Russian historiography of previous periods and the accumulated broad factual basis, modern authors expand the thematic field of research based on the modernization approach also in the discourse of civil society, introducing documentary material of predominantly regional nature into scientific circulation. On the basis of the study of modern publications, the authors’ approaches to the periodization of cooperation are determined. It is established that in most regional works the problems of periodization are not considered as a scientific task, however, the author's approaches are obvious from the structure of the works and the explanation of the factors of development of cooperation. Using the example of regional documentary material, the authors specify the periodization, based on the specifics of the socio-economic and socio-political development of the region under study. Modern authors propose different criteria for periodization and, when analyzing the process of cooperative construction on the example of certain types of cooperation and the activities of cooperative organizations as well as in the framework of regional studies, they also focus on the time of the origin of the movement, on the starting points of quantitative growth of cooperative organizations and strengthening their legal and organizational foundations.
A social media study: the state civil apparatus commission’s online presence
Authors: Suswanta  , Pratama Irfandi , Rahmat Al Fauzi
Published: December 2024

The State Civil Apparatus (ASN) Law exists as a bureaucratic reform movement to strengthen institutions. One of which is the formation of the State Civil Apparatus Commission (KASN) to monitor and evaluate ASN management. This enthusiasm is present in filling high bureaucratic positions not based on a merit system that is in addition to the issue of bureaucratic politicisation, which refers to violations of the code of ethics. Still, regarding this issue, the urgency of KASN is often questioned to the point of discussing its dissolution. Thus, this research examines the existence of KASN using qualitative methods with a descriptive approach, using primary data obtained from KASN's Twitter social media account with the username @KASN_RI by capturing it using Ncaputre software with data results from 2017-2021. For data analysis, the researchers used Software Nvivo 12 Plus with the Word Cloud tools menu, limited to 100 display words with three minimum lengths. Research findings show that KASN is more active in providing information on Twitter social media regarding merit system policies, neutrality, and ASN codes of ethics and behaviour. This is in line with the findings, which show that the words that frequently appear are KASN and ASN. Then, to increase the visibility of information, mention working partners, including the PANRB and BKN ministries. Then, the hashtags used are varied, but their use does not explicitly indicate a related event, so the hashtag function is not utilised correctly.
Hajat lembur: a disaster literacy on ritual communication on the Lembang fault
Authors: Rosfiantika Evi , Aunillah Rinda , Gustaman Budi
Published: December 2024

The Hajat Lembur is a yearly ritual by Batu Lonceng Villagers, in West Bandung-Indonesia, following the 1956 landslide disaster that occurred in the Lembang Fault area. A series of local wisdom emerged as important messages tucked into the procession that are related to the ritual communication concept.  Based on the three interrelated terms of ritual communication: communication, communion, and commonality (Couldry,2003), this research seeks to reveal the meaning of the Hajat Lembur tradition as disaster risk reduction literacy. This research uses a case study approach, conducted by participatory observation in the Hajat Lembur ritual procession in 2023; in-depth interviews with two informants, in the form of opinion leaders who play a central role in the ritual procession; and literature reviews. The results showed that in terms of communication, the Hajat Lembur tradition is interpreted as a transcendent medium about the importance of maintaining harmonisation between humans, nature, and the Creator. In terms of communion, it is a celebration of the sacred Batu Wahyu that led to an agreement to establish a sacred forest, as an area that must be preserved. In terms of commonality, this ritual is a joint expression of the Batu Lonceng Village community about the importance of protecting nature and the environment inherited by the ancestors. Although there is a need for more established local knowledge in this area, this research contributes to the future of disaster mitigation management by emphasising rationality and local wisdom in dealing with natural disasters.
New media: dinamika neo-rasisme di Twitter
Authors: Ritonga Hasnun Jauhari , Sampurna Ahmad , Fandhy Arya
Published: December 2024

This research aims to understand and evaluate the climate of neo-racism in Indonesia, focusing on discriminatory experiences within a heterogeneous society. The study employs a qualitative and content analysis approach, utilising 7.097 tweets about racism as primary data and a literature review as secondary data. In the analysis process, the researchers used Netlytic software to identify patterns or trends of racism on Twitter. The obtained data were then reconstructed to ensure validity. Subsequently, the data were reduced, presented, and concluded to identify the racism climate on Twitter accurately. The findings of this study reveal that the dynamics of neo-racism on Twitter reflect deep-seated issues regarding society's understanding of racism. In Indonesia, the legacy of colonialism and identity politics exacerbates this situation, while regulations and law enforcement remain ineffective in addressing this issue. Analysis of netizens' interactions shows that much content focuses on accusations and validation without concrete solutions. Therefore, comprehensive efforts involving robust regulation, social media ethics education, and cooperation between the government, social media platforms, and the community are necessary. These efforts aim to create an inclusive environment that respects diversity, while also building collective awareness of the importance of tolerance and eliminating discrimination.
Narrative analysis of 'skandal mahkamah keluarga' in Tempo magazine, October 2023
Authors: Alfarisi Salman , Ayu Puspitasari Fingky
Published: December 2024

This research involves a narrative analysis of the news text published in the October 22, 2023, edition of Tempo magazine titled 'Skandal Mahkamah Konstitusi' The Constitutional Court granted the age requirement for presidential and vice-presidential candidates, stipulating that they must be at least 40 years old or have experience as a regional head. As the 2024 Presidential General Election drew nearer, the case became highly controversial. This research explores how Tempo framed its narrative in reporting the Constitutional Court Lawsuit, utilising the Algirdas Greimas model for narrative analysis. The study is qualitative and descriptive. The discussion results reveal that in that edition, Tempo, specifically reported the involvement of Anwar Usman, the Chairman of the Constitutional Court. Anwar Usman was portrayed as the party attempting to navigate the lawsuit with various news narratives, such as 'manoeuvring' and 'lobbying,' during the trial process. Tempo aimed to convey the message that the decision raised various complicated issues and reflected the complex dynamics within the Constitutional Court. Tempo, as a news source, plays a critical role in uncovering hidden realities that can only be revealed through in-depth investigation and extracting information from internal sources within the Constitutional Court. This is Tempo's main strength.
Reducing divorce rates: the role of the communicative religious leader in Aceh
Authors: Halihasimi  , M.S. Anwar , Rizki Fauza
Published: December 2024

This study examines the function of da'wah communication in disseminating knowledge, education, and counsel to mitigate divorce rates in Central Aceh Regency. This study incorporated A descriptive qualitative methodology, specifically observation, interviews, and document analysis of pertinent literature, legislation, regulations, and scholarly publications     . This methodology provided comprehensive insights into the intricate dynamics of da'wah communication and its influence on marital stability. The study investigated three principal questions: What impact does da'wah communication as an information provider have on divorce rates? How does its educational role affect marital stability? How does its advisory role aid in decreasing divorce rates in the Central Aceh District? The results demonstrate that da'wah communication significantly lowers the divorce rate in Central Aceh District by functioning as a source of knowledge, education, and guidance; effectively resolving family disagreements is essential for fostering patience and encouraging spouses to uphold marital integrity under divine guidance. In contrast, families who do not engage in da'wah communication are more susceptible to issues, misunderstanding, and divorce. These findings underscore the need to incorporate da'wah communication into community initiatives to mitigate divorce rates and promote healthier family relations. Future studies may investigate the enduring impacts of da'wah communication on marital stability and its possible implementation in many cultural settings.
Skincare shopping: cultural industry's influence on Indonesian metrosexual body discipline
Authors: Putranto Teguh Dwi
Published: December 2024

The research aims to discover the cultural industry's power by looking at body discipline practices in online skincare shopping activity among metrosexuals. Using virtual ethnography, we focus on Instagram postings that focus on digital text as a medium for seeing the form of discipline of metrosexual men. Regarding the research informants, the researcher determined six informants. The characteristics of these informants in this study are male, living/residing in Jakarta, Bandung, and Surabaya. Males interested in facial skincare products often. purchase through online shopping (repetition buying). Informants were obtained through a search using the keyword ‘metrosexual’ in the Instagram search field by and examined profiles that contain some information. Search results are sorted only among Indonesian metrosexuals. Data analysis began with observation of the informant's Instagram account profile from the Text/Photo/Video/Comment that can be accessed publicly. Followed by interviews to explore the understanding of the meaning behind the practice of online shopping through Instagram posts. The results of this study show that by developing self-care expertise, the cultural industry and body discipline of metrosexual men through online skincare buying activities contribute to disciplining their bodies. Disciplinary power is applicable to metrosexual males who receive discipline from facial skincare product businesses as well as from guys who post about these products. According to Adorno's research, social media is used as a disciplining tool by the cultural business for metrosexual guys, and this is perceived through the lens of the panopticon mechanism.
Технології моделювання і оцінки ризиків інформаційної безпеки
Authors:   Національний університет водного господарства і природокористування, Карпович Іван , Гладка Олена ,   Національний університет водного господарства і природокористування, Бухало Юрій ,   Національний університет водного господарства і природокористування
Published: December 2024

Виконано моделювання, аналіз і оцінювання ризиків інформаційної безпеки на основі прикладних аспектів теорії графів в поєднанні з експертними методами оцінювання. Проаналізовано сучасні підходи до моделювання заходів та методики оцінки ризиків в інформаційній безпеці. Сформульовано рекомендації щодо підвищення ефективності системи захисту інформації. Запропонована модель може бути застосована на етапі аудиту безпеки організації для виявлення слабких місць системи захисту, а також для удосконалення діючої системи кіберзахисту.
The Republic of Vietnam’s Diplomatic Opportunities during the War of 1965–1973
Authors: Ha Trieu Huy
Published: December 2024

This study uses a technique known as a literature review to investigate contemporary derivative sources and arguments addressing the diplomatic history of the Republic of Vietnam in relation to Free World Military Forces during the Vietnam War (1965–1973). The primary discovery of this study is that, despite extensive discussion about the conflict and the involvement of allied troops, a significant gap remains in the diplomatic history of the Republic of Vietnam, despite a gradual increase in attention within international scholarly circles. The purpose of this report is to compile relevant publications and make suggestions for additional research to fill in the gaps that exist in the existing literature on the Vietnam War around the world.
About the Second Koguryo Capital
Authors: Stoyakin M. A.
Published: December 2024

The article discusses the current issue of studying the second capital of the early medieval state of Koguryo. It reviews and generalizes the limited and somewhat outdated Russian historiography on the topic. In order to reveal the unique features of the capital during the middle period, key Korean and Chinese written and epigraphic sources were examined and analyzed. According to the written data of the “Samguk Sagi”, the existence of the second capital is connected to the period between 3–427 AD. Information regarding the location of the capital remains unclear until the end of the Koguryo period and is only revealed in the subsequent Bohai period. The analysis of written sources is complemented by archaeological data of the ancient Guonei city and Wandu Mountain City located in the Jilin province of China, which correlate with the capital’s sites. Guonei city located in modern Jian city was a large centre; the perimeter of the walls was approximately 2.74 kilometres. In the central part, buildings correlating to administrative or palace buildings were excavated. At the nearby Wandu Mountain City, the perimeter of the walls was approximately 7 kilometres, and a large palace complex stretching over four terraces was discovered. Archaeological information indicates a different time period of the capital as a fortified city, and confirms its functioning as a centre of the district and Huanzhou county throughout the Bohai period. It is believed that initially, the Koguryo leader established a military headquarters in a hard-to-reach mountain valley near Yalu river, which evolved into a big administrative and political centre as the state developed. Further archaeological surveys are needed in the Koguryo capital area to establish a detailed chronology and better understand the futures of the Koguryo capital, and to further explore the connection between archaeology and early chronicle information.
Силилирование с генерацией фиксированного заряда для анализа спиртов методом масс-спектрометрии МАЛДИ и ПАЛДИ
Authors: Жиляев Дмитрий Игоревич , Воскресенский Леонид Геннадьевич , Половков Николай Юрьевич , Борисов Роман Сергеевич
Published: December 2024

Предложен новый способ дериватизации спиртов для их анализа методом масс-спектрометрии с матрично-активированной (МАЛДИ) и поверхностно-активированной лазерной десорбцией/ионизацией (ПАЛДИ). Подход основан на силилировании аналитов бромметилхлордиметилсиланом в присутствии амина с образованием соединений с ковалентно-связанным зарядом. На примере серии алифатических, терпеноидных и стероидных спиртов показано, что дериватизация протекает количественно, а регистрируемые в условиях МАЛДИ  и ПАЛДИ масс-спектры содержат интенсивные пики ионов, соответствующие катионным частям получаемых производных.

A new method of derivatization of alcohols for their analysis by matrix-assisted laser desorption/ionization (MALDI) and surface-assisted laser desorption/ionization (SALDI) is proposed. The approach is based on the silylation of the analytes by bromomethylchlorodimethylsilane in the presence of tertiary amines to yield the fixed-charge containing derivatives. The proposed method was tested using aliphatic, terpenoid and steroid alcohols. MALDI and SALDI mass spectra of all derivatives contained intense ion peaks corresponding to their cationic parts.
Новые масс-спектрыМасс-спектры электронной ионизации триметилсилил-пентафторпропионильных и триметилсилил-гептафторбутирильных производных β-адренорецепторных модуляторов
Authors: Самосорова Анна Александровна , Ефимова Юлия Александровна
Published: December 2024

В работе предложено масс-спектрометрическое определение бета-адренорецепторных модуляторов посредством бифункциональной дериватизации при помощи N-метил-N-(триметилсилил)-трифторацетамида с последующим ацилированием пентафторпропионовым или гептафтормасляным ангидридами с описанием полученных производных.

The paper presents proposals for mass spectrometric determination of beta-adrenoreceptor modulators by bifunctional derivatization with N-methyl-N- (trimethylsilyl)-trifluoroacetamide followed by acylation with pentafluoropropionic or heptafluorobutyric anhydrides with a description of the derivatives obtained.
Определение фосфонилированного тирозина как маркера экспозиции к фосфорорганическим отравляющим веществам в сухих образцах плазмы крови методом ВЭЖХ-МС/МС высокого разрешения
Authors: Корягина Н.Л. , Алюшина Т.И. , Каракашев Г.В. , Савельева Е.И. , Хлебникова Н.С. , Радилов А.С.
Published: December 2024

Разработана методика определения фосфонилированного тирозина – маркера экспозиции к фосфорорганическим отравляющим веществам (ФОВ), в сухих образцах плазмы крови методом тандемной жидкостной хроматомасс-спектрометрии высокого разрешения. Показана стабильность сухих образцов при хранении в течение 2-х месяцев при температурах 22 и 40 оС при использовании в качестве носителя бумаги «Protein Saver 903 Card». Установлено, что температура хранения оказывает значительное влияние на стабильность сухих образцов: при повышенной температуре содержание определяемых соединений снижается практически в три раза в сравнении с такими же образцами, хранившимися при комнатной температуре. Показано, что в отсутствие специальной бумаги возможно использовать в качестве носителя фильтровальную бумагу «синяя лента». При этом аддукты ФОВ с тирозином надежно определяются через 1 месяц после хранения образцов при комнатной температуре (22 оС). В условиях повышенных температур (40 оС) использование фильтровальной бумаги не рекомендуется. Предложенный способ хранения и анализа образцов позволяет отбирать большие серии образцов и легко транспортировать их, а также автоматизировать процесс анализа. Сухие пятна могут доставляться в лаборатории по всему миру в обычных конвертах без обязательных для жидких биообразцов согласований.

A procedure for the determination of phosphonylated tyrosine, a marker of exposure to organophosphorus nerve agents, in blood plasma dry spot (DBS) samples by means of liquid chromatography/tandem high resolution mass spectrometry was developed. The DBS samples on Protein Saver 903 Card substrates were shown to be stable for 2 months on storage at 22 and 40 оС. It was found that the temperature of storage has a high impact on the stability of the DBS samples: the concentration of the analyte in the samples stored at 40 оС was about 3 times lower compared to the samples stored at room temperature. It was shown that the special Protein Saver 903 Card substrates could be replaced by an ordinary blue ribbon filter paper. In the latter case, the tyrosine adducts of nerve agents were reliably determined in the DBS samples stored for 1 month at room temperature (22 оС). However, for storage at elevated temperatures (40 оС), the use of filter paper substrates is not recommended. The proposed approach to storage of blood samples allows one to take and easily transport large series of samples and automate their analysis.
ХАРАКТЕРИЗАЦИЯ СТРУКТУРНЫХ ФОРМ БИОМОЛЕКУЛ НА ОСНОВЕ ДЕКОМПОЗИЦИИ И РАЗДЕЛЕНИЯ ЗАРЯДОВЫХ РАСПРЕДЕЛЕНИЙ ИХ ИОНОВ
Authors: Разников В.В. , Разникова М.О.
Published: December 2024

Целью настоящей работы является эффективное выявление и анализ скрытой информации в масс-спектрометрических данных о структурном составе исследуемых растворов биомолекул при их электрораспылении. В работе приводится описание алгоритма последовательного разделения, совмещенного с декомпозицией измеренного зарядового распределения биомолекул, при увеличении числа компонент, соответствующих независимому присутствию носителей заряда с найденными вероятностями удерживания для заданного числа ионогенных групп этих биомолекул. Обсуждаются результаты расчетов – одномерная и двумерная  декомпозиция зарядовых распределений  цитохрома с с разделением на компоненты,  предположительно соответствующие различным  конформациям белка. Впервые разработаны метод и программа для поиска  компонент и их вкладов в масс-спектры многозарядных ионов, когда масс-спектры этих компонент заранее не известны.

The aim of this work is the efficient disentangling and analysis of hidden information in mass spectrometric data on the structural composition of the studied solutions of biomolecules in their electrospray ionization. This paper provides a description of the algorithm of the sequential splitting combined with a decomposition of the measured charge state distributions of biomolecules, while increasing the number of individual components corresponding to the independent presence of charge carriers with the found probabilities of retention for a given number of ionogenic groups of these biomolecules. The article discusses the results of calculations for two-dimensional and one-dimensional decomposition of the charge distributions of cytochrome с with separation, presumably corresponding to different conformations of proteins. For the first time it was developed a method and tool for searching individual components and their contributions to the mass spectra of multiply charged ions, when the mass spectra of these components are not known in advance.
ИЗУЧЕНИЕ КИНЕТИКИ СТАРЕНИЯ РУКОПИСНЫХ ШТРИХОВ МЕТОДОМ ВЫСОКОЭФФЕКТИВНОЙ ЖИДКОСТНОЙ ХРОМАТОГРАФИИ С МАСС- СПЕКТРОМЕТРИЧЕСКИМ ДЕТЕКТИРОВАНИЕМ
Authors: Байгильдиева Д.И. , Крылова А.С. , Байгильдиев Т.М. , Шпигун О.А. , Родин И.А.
Published: December 2024

Одной из центральных задач технико-криминалистического исследования документов является установление времени и последовательности нанесения записей в документ такими распространенными средствами письма, как шариковые ручки. Для её решения требуется получить информацию о составе компонентов паст шариковых ручек, которыми исполнялись записи, о том, какие качественные и количественные изменения претерпели компоненты во времени после нанесения записей на документ, а также о том какие изменения наблюдались с компонентами чернил под воздействием окружающей среды либо под воздействием внешних источников таких как фото-, термо-, УФ-, ИК-, микроволновых излучений. В этой связи, целью данной работы являлось развитие подхода, позволяющего выявлять изменения в составе паст ручек, протекающих в штрихах с течением времени, а также установление временных зависимостей, с помощью которых стала бы возможной оценка срока давности исполнения записей. В результате работы оценены возможности жидкостной хроматографии/масс-спектрометрии при обнаружении изменений в составе паст шариковых ручек BIC Original, Corvina 51 и Pilot. Проведено моделирование процессов деградации красителей при воздействии на штрихи УФ-излучения и определены кинетические параметры, описывающие степень превращения красителей.

One of the central problems of documents forensic examination is the determination of the ink deposition date by such common writing instruments as ballpoint pens. To solve this problem, it is necessary to get information about the composition of ballpoint pen inks that were used for depositing inks, to get information about qualitative and quantitative changes of components undergoing in time after depositing inks on a document, as well as to know changes in the composition and properties of the ink caused by environmental influences or intentional effects on document such as photo-, thermal-, UV-, IR-radiations, microwave and other types of external influences. Therefore, the purpose of this research was to develop an approach that allows one to detect changes and establish dependence of these changes in the ballpoint pen composition that occur in strokes over time. As a result of this study, the possibilities of liquid chromatography/mass spectrometry for the detection of changes over time in the composition of ballpoint pen inks BIC Original, Corvina 51 and Pilot were estimated. Modeling of the dyes degradation under the influence of UV radiation on strokes was carried out. Kinetic parameters that describe the degree of dyes conversion were determined.
Исследование водного хлорирования ультрафиолетового протектора авобензона в присутствии неорганических солей методом газовой хроматографии в комбинации с масс-спектрометрией высокого разрешения
Authors: Детенчук Е.А. , Чэнь Ц. , Полякова О.В. , Требше П. , Покрышкин С.А. , Лебедев А.Т.
Published: December 2024

Одной из важнейших стадий водоподготовки является ее обеззараживание. Это касается не только питьевой воды, но и, например, воды бассейнов. Самый распространенный метод для устранения микроорганизмов на сегодняшний день - это хлорирование. Однако реакции активного хлора с природными и антропогенными соединениями, растворенными в воде, приводит к образованию значительного числа химических соединений, многие из которых могут нанести вред человеку. Среди антропогенных соединений, ассортимент которых все возрастает год от года, определенную нишу занимают УФ-фильтры. Их относят к новым экотоксикантам, а их применение для защиты кожи набирает популярность во всем мире. В данной работе методом газовой хроматографии в сочетании с масс-спектрометрией высокого разрешения идентифицировано более 30 соединений, включая значительное число галогенпроизводных, образующихся в результате водного хлорирования популярного УФ фильтра авобензона в воде в присутствии неорганических солей меди, железа, а также бромидов и иодидов. Исследования показали значительные вариации в качественном и количественном составе продуктов реакции в зависимости от добавки. Важно отметить образование бром- и иодсодержащих продуктов.

Disinfection is an important technological step in water treatment procedure. Besides drinking water it involves also, for example, swimming pool water. Aquatic chlorination is most widespread procedure nowadays. It efficiently eliminates micro organisms. However active chlorine reacting with natural and anthropogenic compounds dissolved in water gives rise to the formation of numerous compounds which may be hazardous for human health. The assortment of anthropogenic contaminants grows every year, while UV-filters are well represented among them. Since recently they are referred as emerging contaminants while their application for the skin protection becomes more and more popular all over the world. Over 30 compounds including numerous halogenated derivatives forming due to aquatic chlorination of a popular UV filter avobenzone in the presence of inorganic salts of iron and copper as well as bromides and iodides were identified by means of gas- chromatography/ high resolution mass spectrometry. The study demonstrated notable variation of the qualitative and quantitative composition of the reaction products depending on the added salt. It is worth mentioning the formation of brominated and iodinated products.
Времяпролетный масс-спектрометр ЭМГ-40-3 – новый высокочувствительный прибор для работы с легкими газами, в том числе, тритийсодержащими
Authors: Пилюгин И.И. , Ждан В.Т. , Козловский А.В.
Published: December 2024

Приведены технические характеристики времяпролетного масс-спектрометра ЭМГ-40-3. Показано, что чувствительность прибора практически на два порядка превышает чувствительность прибора ЭМГ-40-2. Разрешающая способность прибора обеспечивает реализацию методики количественного анализа состава газовой смеси, содержащей изотопы гелия и водорода, в том числе, тритий.

Technical characteristics of time-of-flight mass spectrometer EMG-40-3 are given. It is shown that  the instrument sensitivity is two orders of magnitude more than that of EMG-40-2. Resolving power of instrument provides the quantitative on-line analysis of gas mixtures containing helium and hydrogen isotopes as well as tritium.
Аналитические потенциалы для эффективного моделирования планарных и осесимметричных ионных зеркал
Authors: Бердников А.С. , Веренчиков А.Н. , Кириллов С.Н. , Помозов Т.В. , Хасин Ю.И. , Явор М.И.
Published: December 2024

В работе получены аналитические выражения для электростатических полей планарных и осесимметричных ионных зеркал с кусочно-постоянными и кусочно-линейными потенциалами на поверхностях электродов, лежащих симметрично в параллельных плоскостях или на цилиндрической поверхности, а также при наличии дополнительного торцевого электрода. Результаты могут быть использованы для эффективной оптимизации аберрационных свойств времяпролетных масс-анализаторов и электростатических ионных ловушек с бессеточными ионными зеркалами на основе как стандартных плоских или кольцеобразных электродов с конечными зазорами между ними, так и печатных плат.

In the paper, analytical expressions for electrostatic fields of two-dimensional planar and axially symmetric mirrors with piecewise-constant and piecewise-linear potentials at  electrode surfaces located symmetrically in parallel planes or on a cylindrical surface, in particular in presence of an additional cap electrode obtained are. The results can be used for efficient optimization of aberration properties of time-of-flight mass analyzers and electrostatiс ion traps with gridless ion mirrors based on standard planar or ring-type electrodes with finite gaps between them, as well as on PCB plates.
Дериватизация с генерацией фиксированного заряда для анализа карбонильных соединений методом масс-спектрометрии с ионизацией электрораспылением
Authors: Ильюшенкова В.В. , Жиляев Д.И. , Куликова Л.Н. , Горяинов С.В. , Борисов Р.С.
Published: December 2024

Представлены результаты применения дериватизации с использованием N,Nдиметиламиноалкиламинов в сочетании с алкилгалогенидами для анализа алифатических альдегидов и кетонов, а также кетостероидов методом массспектрометрии с ионизацией электрораспылением (ИЭР). Обе стадии реакции протекают количественно, а массспектры ИЭР характеризуются высокоинтенсивными пиками соответствующих аммонийных катионов. Распад последних в условиях активации соударением в большинстве случаев сопровождается только отщеплением концевой триалкиламиногруппы в виде нейтрального осколка. Такая селективная фрагментация может быть использована для разработки высокочувствительного метода на основе мониторинга выбранных реакций.

Fixedcharge generation derivatization approach based on a reaction with N, Ndimethylaminoalkylamines followed by quaternization with alkyl halides was applied for the analysis of aliphatic aldehydes and ketones, as well as ketosteroids by electrospray ionization (ESI) mass spectrometry. Both stages of the reaction proceed quantitatively, and registered ESI mass spectra of the products contain abundant peaks of the corresponding ammonium cations. In most cases, the dissociation of the latter under collision activation results only in a loss of terminal trialkylamino group in the form of a neutral. Such predictable fragmentation can be used for developing of highly sensitive methods of analysis based on selected reaction monitoring.
Ионно-оптическая схема малогабаритного масс-спектрометра для изотопного анализа водородно-гелиевых смесей
Authors: Саченко В.Д. , Якушев Е.М. , Назаренко Л.М. , Антонов А.С. , Галль Л.Н. , Галль Н.Р. , Бердников А.С.
Published: December 2024

Обсуждается ионнооптическая схема (ИОС) массспектрометра, предназначенного для изотопных измерений легких газов, содержащая магнитную призму с плоскопараллельными полюсными наконечниками и два секторных, одинаковых по геометрии цилиндрических конденсатора, симметрично расположенных относительно призмы. Приведены соотношения между геометрическими параметрами конденсаторов и призмы, необходимые для обеспечения ахроматической фокусировки ионного пучка в плоскости приемной щели детектора. Проведена численная оценка влияния аберраций ИОС на разрешающую способность ИОС по массам и определен оптимальный эмиттанс ионного пучка на выходе из источника, позволяющий достичь разрешение на уровне величин порядка 3000 3500 при выходной щели ионного источника порядка 50 мкм, высоком пропускании массанализатора, малых габаритах магнитной призмы и умеренной общей длине центральной ионной траектории (менее одного метра). ИОС характеризуется высокой дисперсией по массам, значительно превосходящей по величине дисперсию традиционных секторных магнитных массспектрометров тех же габаритов. В составе ИОС использованы достаточно простые по геометрии элементы, что снижает затраты при ее реализации и делает массспектрометр технологичным и удобным в настройке и эксплуатации.

The ionoptical scheme (IOS) of a mass spectrometer intended for isotopic measurements of light gases is discussed. The mass spectrometer contains a magnetic prism with planeparallel pole pieces and two sectorsized cylindrical capacitors with identical geometry that are symmetrically located relative to the prism. The relations between the geometrical parameters of the capacitors and the prism, which are necessary to ensure the achromatic focusing of the ion beam in the plane of the receiving slit of the detector, are given. A numerical evaluation of the influence of IOS aberrations on the IOS resolution by mass is carried out. The emittance of the ion beam at the output from the source is optimized carefully. It produces the output resolution 30003500 with an output gap of the ion source of about 50 m, high transmittance of the mass analyzer, small dimensions of magnetic prism and a moderate total length of the central ion trajectory (less than one meter). Mass spectrometer is characterized by a high dispersion in mass, which is much higher than the dispersion of traditional sector magnetic mass spectrometers of the same dimensions. It contains the elements that are fairly simple in geometry are used, which reduces costs in its implementation and makes the mass spectrometer technologically advanced and easy to set up and operate.