bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of TM and CM on Organizational Leadership in Foreign Entities of MNCs
Authors: Haromszeki Łukasz
Published: June 2023

Background

Foreign direct investment (FDI) of multinational corporations (MNCs) from Central Europe have become more visible in the last decade, but many studies describing the role of talent management (TM), competency management (CM), and organizational leadership (OL) in MNCs have taken place in enterprises with headquarters in developed countries. The relationship between different variables in MNCs headquartered in less developed countries has not been satisfactorily explained in the scientific and professional literature.

Research aims

Hence, the main aim of this paper is based on the outcomes presentation of the relations between TM, CM, and the advancement level of OL practices in MNCs having their headquarters in less developed countries.

Methodology

The theoretical and empirical suppositions are based on two major concepts: resource-based views and a situational approach. The data were collected using the computer-assisted telephone interviewing (CATI) method in 200 nonfinancial enterprises with the domination of the Polish capital having foreign entities, inter alia, in Central and Western Europe.

Findings

The conducted research shows that there is a relationship among TM, CM, and OL practices. Also, there are significant differences between companies depending on the duration of the MNC’s operation on the market, the size of the organization, and the type of business profile. Whereas the assumptions have been based on a literature review, empirical research has not confirmed that there is a difference between foreign subsidiaries located in more and less developed countries (here, Central Europe and Western Europe).
Protein sequence features of H1N1 swine influenza A viruses detected on commercial swine farms in Serbia
Authors: Zorić Jelena Maksimović , Veljović Ljubiša , Radosavljević Vladimir , Glišić Dimitrije , Kureljušić Jasna , Maletić Jelena , Savić Božidar
Published: June 2023

Introduction

Swine influenza A viruses (swIAVs) are characterised by high mutation rates and zoonotic and pandemic potential. In order to draw conclusions about virulence in swine and pathogenicity to humans, we examined the existence of molecular markers and accessory proteins, cross-reactivity with vaccine strains, and resistance to antiviral drugs in five strains of H1N1 swIAVs.

Material and Methods

Amino acid (AA) sequences of five previously genetically characterised swIAVs were analysed in MEGA 7.0 software and the Influenza Research Database.

Results

Amino acid analysis revealed three virus strains with 590S/591R polymorphism and T271A substitution within basic polymerase 2 (PB2) AA chains, which cause enhanced virus replication in mammalian cells. The other two strains possessed D701N and R251K substitutions within PB2 and synthesised PB1-F2 protein, which are the factors of increased polymerase activity and virulence in swine. All strains synthesised PB1-N40, PA-N155, PA-N182, and PA-X proteins responsible for enhanced replication in mammalian cells and downregulation of the immune response of the host. Mutations detected within haemagglutinin antigenic sites imply the antigenic drift of the five analysed viruses in relation to the vaccine strains. All viruses show susceptibility to neuraminidase inhibitors and baloxavir marboxil, which is important in situations of incidental human infections.

Conclusion

The detection of virulence markers and accessory proteins in the analysed viruses suggests their higher propensity for replication in mammalian cells, increased virulence, and potential for transmission to humans, and implies compromised efficacy of influenza vaccines.
Perceptions and Experiences After Participating in a Two-Year Outdoor Adventure Programme
Authors: Lazaridis Alexandros , Syrmpas Ioannis , Krommidas Charalampos , Digelidis Nikolaos
Published: June 2023

Physical activity levels are alarmingly low and new strategies must be adopted to prevent this trend. This study focuses on examining the impact of an outdoor adventure education programme on adolescents. The participants were 75 adolescent students aged 12–14. The programme lasted two school years. Students participated in extracurricular schoolyard outdoor activities and outdoor adventure activities conducted during the two one-day and one four-day trips in both school years. A qualitative methodology was used with data collected using semi-structured interviews. At the end of each school year, 12 students were interviewed. Data analysis revealed that the programme promoted basic psychological needs. More specifically, adolescents reported that schoolyard activities and outdoor adventure activities promoted their satisfaction with autonomy, competence and relatedness. Interestingly, the programme gave girls the autonomy to choose activities that promoted their competence and interaction with peers in a better way than a typical PE lesson. Modifying existing curricula with innovative practices or planning new ones could improve the general quality of life.
Design, Modelling and Research of an Antenna System for Transmitting and Receiving Information in Satellite Systems
Authors: Islamov Islam , Safarli Arzu
Published: June 2023

The work deals with the design, modelling and research of an antenna system for transmitting and receiving information in satellite systems. It was revealed that this antenna array with a transmitter of 85 dBWt is guaranteed to solve the target problem of providing mobile satellite communications with both one global beam and a plurality of beams with a width of 0,7 × 0,7°. It should be added that the advantage of forming multiple beams compared to a global beam with a single phased antenna array is: higher data rate, relatively low requirements for antenna systems of ground stations, greater noise immunity of the radio link, the ability to dynamically control the signal power in each beam.
Healthcare utilization of patients with hereditary angioedema treated with lanadelumab and subcutaneous C1-inhibitor concentrate
Authors: Riedl Marc A. , Hinds David R. , Prince Patricia M. , Alvord Trevor M. , Dosenovic Sara , Abdelhadi Jenna F. , Brownrigg Jack R. , Camp Charlotte L. , Machnig Thomas , Banerji Aleena
Published: June 2023

Background:New hereditary angioedema (HAE) treatments have become available in recent years for the treatment of HAE due to C1-inhibitor (C1-INH) deficiency, including two subcutaneous (SC) options: a monoclonal antibody (lanadelumab) and a plasma-derived C1-INH concentrate (SC-C1-INH). Limited real-world data on these therapies have been reported.Objective:The objective was to describe new users of lanadelumab and SC-C1-INH, including demographics, healthcare resource utilization (HCRU), costs, and treatment patterns before and after beginning treatment.Methods:This was a retrospective cohort study that used an administrative claims data base. Two mutually exclusive cohorts of adult (ages ≥18 years) new users of lanadelumab or SC-C1-INH with ≥180 days of continuous use were identified. HCRU, costs, and treatment patterns were assessed in the 180-day period before the index date (new treatment use) and up to 365 days after the index date. HCRU and costs were calculated as annualized rates.Results:Forty-seven patients who used lanadelumab and 38 patients who used SC-C1-INH were identified. The most frequently used on-demand HAE treatments at baseline were the same for both cohorts: bradykinin B2antagonists (48.9% of the patients on lanadelumab, 52.6% of the patients on SC-C1-INH) and C1-INHs (40.4% of the patients on lanadelumab, 57.9% of the patients on SC-C1-INH). More than 33% of the patients continued to fill on-demand medications after treatment initiation. Annualized angioedema-associated emergency department visits and hospitalizations decreased after initiation of treatment, from 1.8 to 0.6 for the patients on lanadelumab and from 1.3 to 0.5 for the patients on SC-C1-INH. Annualized total healthcare costs after treatment initiation in the database were $866,639 and $734,460 for the lanadelumab and SC-C1-INH cohorts, respectively. Pharmacy costs accounted for >95% of these total costs.Conclusion:Although HCRU decreased after the initiation of treatment, angioedema-associated emergency department visits and hospitalizations and on-demand treatment fills were not completely eliminated. This indicates ongoing disease and treatment burden despite use of modern HAE medicines.
رەنگڤەدانا گرێیا گریسڵدایێ، د چیرۆکا کەچ و باڤ دا، یا حەسەنێ مەتێ
Authors: Sagvan Ahmad Omed
Published: June 2023

ئەڤ ڤەکولین ژ دوو تەوران پێک دهێت، د تەوەرێ ئێکێ دا باس ل چەمک و پێناسەیا گرێیا دەروونى و جۆرێن وێ هاتینە کرن. د تەوەرێ دووەم دا، ڤەکولەر ل سەر وان نیشان و خالان رادوەستە یێن کو دەربرینێ ژ پەیوەندیا سێکسى یا بابێ ب رەنگەک بنهشى بەرامبەر کچێ دکت و ئەو خال یەک ب یەک هاتینە شرۆڤەکرن و دەست نیشان کرن.
Website Evaluation of DISPERDAG Section of the Average Price of Standard Needs Using the WEBQUAL 4.0 Method
Authors: Saifulloh Saifulloh , Zulfa Isti Indana
Published: June 2023

DISPERDAG of Madiun City is one of the organizations that has utilized technology. The community can easily access and get price updates for basic necessities at Pasar Besar City of Madiun. The Internet helps support strong governance management, which benefits its effectiveness. Its functions include making it easier to get information, access public services, communicate with the public, and others. DISPERDAG aims to continue to provide quality services. The quality of services provided to the public must complement the implementation of website-based services so that the public continues to use SISKAPERBAPO services. To provide the best service to users, government institutions should prioritize service quality. Therefore, the success of the service quality of the SISKAPERBAPO website has not been known as long as the website is implemented. This is because there has never been an evaluation of website quality based on user satisfaction. It goes without saying that a website that is useful for assisting users in obtaining information must maintain quality in terms of information delivery and user interaction. Good service quality is offered from the user's point of view as well as the service provider's point of view.
Refugee Education in Bangladesh: A Nation-State Paradox
Authors:   Institute of Human Rights and Peace Studies, Mahidol University, Thailand, Karim Mst Umme Habiba Fahmina
Published: June 2023

This article critically analyzes the politically driven exclusion of Rohingya refugees in the national education system of Bangladesh. By employing realist humanitarianism theory, this study examines the inducement of discrimination against refugees in education and the political factors that have influenced such policy choices and formulation in Bangladesh. Qualitative research involving 35 semi-structured interviews produced in-depth information on key informants' experiences, perceptions, and suggestions on refugee education in Bangladesh. This study revealed that excluding refugees from education was a politically plotted decision, and the reason for such exclusion persisted until now because prolonged discrimination against the refugees created local myths about refugees’ eligibility for their human rights to education. The findings of this study provide insights into both academic research and policy analysis in the field of refugee education by highlighting a protracted refugee situation in Bangladesh that political exclusion can create the ambiance to nullify refugees’ human rights to education. The study recommends weighing refugee voices in refugee program design, broader consultation among humanitarian agencies, and policy review to allow refugees to receive formal, official education.
MODALITY REALIZED ON HORTATORY EXPOSITION TEXTS USED IN SENIOR HIGH SCHOOL GRADE XI TEXTBOOK
Authors: Suciati Hani , Sugiarto Bambang Ruby
Published: June 2023

This research is aimed at analyzing types of modality and the interpersonal meaning through modality system realized in hortatory exposition texts that is used by senior high school grade XI textbook. This study employed Systemic Functional Grammar (SFG) based on Halliday and and Matthiessen’s framework since it can seek and identify the level of language structure which makes kinds of meaning. There are five hortatory exposition texts that were analyzed based on modality system and interpersonal meaning. The first finding showed that there was modality system in all of clauses indicating  author’s interpersonal meaning with the use of language value that is different in the texts. The second finding showed that different types of language value and polarity also indicated the author’s interpersonal meaning in the texts. The types of modality which were found in the five texts were dominated by modalization with positive polarity and median value. In addition, this analysis concluded that five texts comprised several modalization and modulation types which occurred in different ranks. This study suggested that further researchers investigate various texts and other aspects of mood and modality. Keywords: hortatory exposition text, interpersonal meaning, modality, textbook
VOCABULARY LEARNING STRATEGIES APPLIED BY EFL LOW AND HIGH ACHIEVERS
Authors: Chamidah Nur Laelatun
Published: June 2023

This study aimed at investigating vocabulary learning strategies (VLSs) applied by EFL low and high achievers and how they applied it. The writer employed a case study conducted in two senior high schools in Ciamis. There were 60 participants involved in this study consisted of 30 low and 30 of high achievers. To answer the research questions, the writer conducted semi-structured interview and administered closed-ended questionnaire based on Schmitt (1997)’s classifications of vocabulary learning strategies. From the results of completed questionnaire, the writer found that metacognitive, determination, and memory strategies were the mostly applied by EFL low and high achievers. Those strategies included using English media, consulting on bilingual dictionary, and studying a sound of word. Furthermore, the data of interview specifically showed that watching subtitled films, listening English songs, consulting on google translate, and asking friends are also involved. Finally, EFL low and high achievers  have to be aware of their own preferred strategies in learning vocabulary to improve their foreign language learning. It is suggested for English teachers to facilitate students in applying their preferred strategies in learning vocabulary. Keywords: good language learner, low and high EFL achievers, vocabulary learning strategies
MALE AND FEMALE EFL JUNIOR HIGH SCHOOL STUDENTS’ PERCEPTION ON THE USE OF MIND MAPPING METHOD TO INCREASE SPEAKING ABILITY
Authors: Fauzi Ifham Muhammad
Published: June 2023

This study investigated perception of male and female junior high school students’ on mind mapping method employing qualitative method. Classroom observation was used to get perception of male and female students before conducting the questionnaire. Interview was administered to 2 male and 2 female students from 20 students. The findings revealed that: 1) the male and female students perceived mind mapping method interesting to increase speaking ability; 2) female students were more active and enthusiastic in learning process to increase speaking ability than male; 3) female students submitted a better results of the assignments given by their teacher in effort to increase speaking ability by using mind mapping method; 4) mapping method is very helpful to increase speaking ability of male and female junior high school. This study suggests that the teacher should master and have a better understanding of mind mapping method with the purpose of equipping students various knowledge and English interest to increase their speaking ability. Keywords: gender, mind mapping, perception, speaking ability,
OVERCOMING STUDENTS’ PROBLEMS IN PRONOUNCING PAST TENSE VERBS BY MEANS OF YOUTUBE VIDEO
Authors: Santosa Junjun Ahmad
Published: June 2023

This study was aimed to discover whether teaching by using Youtube videos could overcome students’ problem in pronuncing past tense verbs and to discover the students’ perceptions on the use of Youtube video as teaching media. This study mixed-method study involved the eight grade students of junior high school in Ciamis Year 2017/2018 at which the sample was 27 students of experimental group and 27 for control group. Pre-test and post-test were employed to collect the quantitative data and close-ended questionnaires to collect the qualitative data. The findings indicated that t-observed was 2.344 with degree of freedom 52 and level of significance 0.05 with two tailed test t-table (2.021). It implied that t-observed was higher than t-table (2.344 > 2.021) resulting in rejecting Null hypothesis. It means that there were significant differences on the students’ pronunciation achievement who were taught before using YouTube video and after using YouTube video. Also, students have positive perceptions toward the use of Youtube video as teaching media to overcome their problem in pronouncing past tense verbs such as feeling more intellegent, interested and effective in learning speaking especially pronunciation. This study suggested that Youtube video as media can be applied in other level of students. Keywords: Pronunciation, Past Tense, Youtube Videos
AN ANALYSIS OF EFL STUDENTS’ TRANSITIVITY PROCESS OF WRITING RECOUNT TEXT
Authors: Rosmayanti Yanti
Published: June 2023

This paper focuses on the analysis of transitivity process contained in EFL students‟ writing recount text and dominant type of transitivity process in EFL students‟ writing recount text. Descriptive qualitative method is used to analyze the Transitivity Process of EFL students‟ writing recount text consisting of collecting data, classifying data, analyzing, and interpreting data. The document of this study is six EFL students‟ writing recount texts and there are 173 data consisting of clauses taken from the text. The finding reveals that there are six types of transitivity process contained in EFL students‟ recount text: material process, mental process, relational process, behavioral process, existential process and verbal process. Material process becomes the first mostly applied with the total number of occurrence 108 (62.43%) from overall data. Mental process becomes the second mostly applied with the total number of occurrence 26 (15.03%) from overall data. Relational process becomes the third mostly applied with the total number of occurrence 21 (12.14%) from overall data. Existential process is applied with the total number of occurrence 12 (6.94%) from overall data. Verbal Process is applied with the total number of occurrence 5 (2.88%) from all data; and the last, behavioral process is applied with the total number of occurrence 1 (0.58%) from overall data. Material Process is the dominant transitivity process types in all the EFL students‟ recount text with the total number of occurrence 108. Based on the findings, it is recommended that, Transitivity process should be applied in the educational field, for example, in the learning writing process, to help students in improving their achievement and their knowledge of in transitivity process. Keywords:  Recount text, Transitivity process
STYLISTIC ANALYSIS ON WALTER SAVAGE LANDOR’S ‘ACON AND RHODOPE; OR, INCONSTANTLY’
Authors: Kulsum Umi
Published: June 2023

This descriptive study is aimed to find out the types and the functions of imagery and to figure out the examples and the functions of archaic words which are found in poem. The documentary data was used in form of poem script “Acon and Rhodope; or, Inconstantly” written by Walter Savage Landor. Content analysis was used by the writer to analyze the content of poem which was adapted from Cohen, et al. (2007, p. 475). The findings showed that there were 174 words or expressions found in poem which were divided into seven types of imagery such as: visual, auditory, gustatory, olfactory, tactile, thermal, and kinesthesia. There were also seven functions of imagery in the poem: 1) visual imagery representing the sense of sight; 2) auditory imagery representing the sense of sound; 3) gustatory imagery or representing the sense of taste; 4) olfactory imagery representing the sense of smell; 5) tactile imagery representing the sense of touch; 6) thermal imagery representing the sense of heat and cold; and 7) kinesthesia imagery appealing to physical sensations of movement, balance, and muscular tension. Also, there were 95 archaic words found in poem to enhance the aesthetical or musical value of the poem, to find out the social life, customs, belief systems and generally cultural richness of the time he emerged, and to make his language old and solemn. It is suggested for the teacher to understand stylistic analysis used by the poets in their poems; and introduce stylistic analysis to the students so that they can improve knowledge of stylistic analysis and understand its meanings. Keywords: archaic words, imagery, stylistic analysis, Walter Savage Landor
LITERACY BASED TEACHING IN READING RECOUNT TEXT BY USING AUTHENTIC MATERIALS
Authors: Widianti Rifa’i Wini , Herlina Rina
Published: June 2023

This study deals with the development of authentic materials in teaching reading recount text in literacy-based teaching. This study was aimed at 1) observing how the teacher develops recount text by using authentic materials in the literacy-based teaching, and 2) figuring out the students’ responses on authentic materials in recount text developed in the literacy-based teaching. This study employed a qualitative method with the type of case study design. By using purposive sampling technique, this study involved 23 students of class VIII-D and the English teacher is the sample of this study. The research instruments used in this study were the classroom observation, interview, and questionnaires. This study used the triangulation to analyze the data. The result revealed that the teacher used only five of seven principles of literacy in developing authentic materials in teaching reading recount text. They are interpretation, collaboration, problem solving, reflection and self-reflection; and literacy involves language use. Furthermore, the use of authentic materials in reading recount text improved the students’ motivation and reading comprehension. Meanwhile, the use of authentic materials also helped them to overcome their difficulties in learning reading, improving students’ vocabulary, and enhancing the students’ attention in learning reading. It can be concluded that the use of authentic materials by using some principles of literacy has given positive effect to the student’s motivation and reading comprehension. Authentic material is one of media which can help and facilitate the students in a reading text. Keywords: Literacy based teaching, reading, recount text, and authentic materials.
THE ANALYSIS OF POLITENESS STRATEGIES ON EFL STUDENTS’ CHATTING GROUP INTERACTIONS
Authors: Tri Amanda Yuli , Herlina Rina , Ratnawati Ratnawati
Published: June 2023

This paper reported the analysis of the practice of politeness strategies used in WhatsApp group chatting interactions among EFL students. Intermediate level of English Education Program in one of private universities in West Java was employed because they are assumed to interact in WhatsApp group chatting. Furthermore, two research questions were formulated such as follows: 1) what kinds of politeness strategies do EFL students apply in WhatsApp chatting group interactions? and 2) what is the most dominant politeness strategy realized in EFL students’ chatting group interactions? This study utilized descriptive qualitative study to analyze and interpret the realization of politeness strategy used by the EFL students in WhatsApp chatting group interactions. The results revealed that the students used four politeness strategy types. They were bald on-record, positive politeness strategy, negative politeness strategy, and bald off-record. Then, the most dominant type of politeness strategy in WhatsApp chatting group interactions was positive politeness strategy (47.1%). Based on the results, it was concluded that the EFL students used the entire types of the politeness strategy in the interactions of group chatting in which positive politeness strategy were used the most frequently by the students. Keywords: Teaching speaking, Team Game Tourna
THE STUDY OF USING VIDEO LYRIC TO MOTIVATE STUDENTS IN LEARNING PASSIVE VOICE
Authors: Pixtilany Nur Saputri Pebby , Friatin Lilies Youlia , Thoyyibah Luthfiyatun
Published: June 2023

This study is aimed to investigate the effectiveness of using video lyric in learning passive voice toward students’ motivation and students’ perception on learning passive voice using video lyric. In collecting the data, the study used qualitative method especially case study; and triangulation method were observation, interview and questionnaire as instruments. This study was conducted at one of Senior High School in Majenang. Purposive sampling was used to select fifty three students as participants. The writer observed the activities in the classroom using naturalistic record and transcribed the conversation using notes. The writer used semi-structure interview and interpreting the answer of interviewee. The last, she gave twelve questions to the students followed by calculating the frequency and the percentage of each statement. The findings revealed that there were several steps used by teacher to give motivation in learning passive voice using video lyric: 1) giving some simulation; 2) asking students to watch some video of song; 3) asking students to sing a song together; 4) asking the students to write the lyric when the teacher explained the the passive voice; and 5) giving a treatment when the students deliver questions. Meanwhile, students perceived learning passive voice using video lyric positively. They joined with peer and other students; they also enjoyed and gave good attitude. Almost all of the students were enthusiastic when the teacher showed the video lyric. They assumed that video lyric was easier to comprehend passive voice in term of structure and formula. In addition, it improved their imagination, increased their critical thinking, creativity, and comprehension of learning experiences using new teaching aids. Using video lyric as teaching aids can help students to keep their interest in learning. It is very important for the teacher to increase their variety tools in learning activities. Keywords: Motivation, Passive voice, Video lyric.
Exploring the Influence of Work-Family Balance and Burnout on Working Mothers' Character Building Efforts in a Post-Pandemic World
Authors: Muafiah Evi , Sofiana Neng Eri , Nurhidayati Maulida
Published: June 2023

Even though the pandemic has ended, working mothers face challenges in achieving work-family balance so that children's character education goes well. This paper looks at the effect of Work-Family Balance (WFB) and burnout on working mothers in carrying out character education for children after the COVID-19 pandemic. The method used is a quantitative method with a survey design. Data was collected from distributing questionnaires on the Google form filled in by female lecturers at the Islamic University in Indonesia. The findings of this study indicate that there is an effect of WFB and burnout on post-pandemic children's character education. Female lecturers at Islamic universities can be categorized as working mothers who can achieve WFB and educate their children well so there are no born-outs. This research shows that even as working mothers, lecturers at an Islamic university can carry out their role in children's education well.
Determinant Factor Analysis of Family Psychological Resilience: A Study of Muslim University Staff in Indonesia
Authors: Syukur Yarmis , Ardi Zadrian , Zahri Triave Nuzila , Putra Ade Herdian
Published: June 2023

A family is a social unit that exists in every culture. Ideally, every family has well enough psychological resilience to achieve prosperity. However, to achieve good psychological resilience, sometimes problems can arise in the family, which is also experienced by Muslim university staff in Indonesia. Three important factors are predicted to correlate with family psychological resilience: family support, economic resilience, and commitment. This study aims to reveal the factors that correlate with the family psychological resilience of Muslim university staff in Indonesia. This study uses a quantitative approach with correlational methods. This research involved 324 Muslim university staff in Indonesia as respondents. The research instruments used were the Family Support Scale, the Family Economic Resilience Scale, the Family Commitment Scale, and the Family Psychological Resilience Scale. Furthermore, the research data analysis used partial least squares structural equation modelling (PLS-SEM) analysis. The results of this study indicate that family support, family economic resilience, and family commitment have a positive correlation and a significant connection with family psychological resilience. These three factors need to be considered in improving the family psychological resilience of Muslim university staff in Indonesia.
Digital eye strain: Time for a break
Authors: Moulick Parthasarathi
Published: June 2023

Digital eye strain (DES) has increased over time due to excessive use of digital devices by school going children and young adults working online from home, due to COVID-19 pandemic. Prolonged screen time impacts their wellness, which produces stress on visual and musculoskeletal system, in addition to circadian rhythm disturbances. Symptoms of dry eyes, eye pain, redness, watering of eyes, burning/itching sensation in eyes, blurred vision, headache, and poor quality of sleep are some of the common problems faced by the affected individuals. Recommendations to alleviate DES include creating an optimal environment while working on digital devices, taking frequent breaks, and limiting daily screen time. Preventive measures can be taken by counseling/public awareness initiated by ophthalmologists/eye healthcare workers and sensitization of teachers and parents so that timely action can prevent the children from getting adversely affected.
Improving football playing skills through teaching games for understanding: A small-sided games approach
Authors: Firmana Iif , Subarjah Herman , Mahendra Agus , Nuryadi Nuryadi , Sofyan Davi
Published: June 2023

Teachers may find difficulty to design assignments that will increase arousal and keep students’ attention during stages that will encourage them critically to reflect on their actions during physical education activities. Designing assignments that increase arousal and maintain students' attention during physical education activities can be a challenging for teachers, often resulting in a reliance on traditional skills-based approaches. This study aims to address this issue by applying the Teaching Games for Understanding (TGfU) model with a small-sided games (SSG) approach to enhance students' football playing skills. The study used 3 vs 3 invasion games to evaluate playing skills, with a game performance evaluation tool (GPET) used as the playing skill instrument. Decisions regarding coding and execution are made in accordance with the subject of the tactical challenge to be solved, including on- and off-ball attackers. The findings revealed that the experimental group’s decision-making ability when passing was 7.97 and when dribbling was 7.28. The performance results in the experimental group were 7.98 and those in the control group were 7.48. The research made a valuable contribution by demonstrating how using TGfU and SSGs can improve football skills and enhance the quality of teaching in physical education. One limitation is that the research only focused on football playing skills, so the results may not be generalizable to other sports or activities. Future research can investigate the impact of the intervention on other outcomes, such as enjoyment, motivation, and participation in physical activity.
In vitro propagation and genetic fidelity analysis of Celastrus paniculatus Willd – a vulnerable high trade medicinal woody liana of Western Ghats
Authors: Saranya Babu Jayaprakash C.M. , Minoo Divakaran , Madhusoodanan P.V.
Published: June 2023

Celastrus paniculatus Willd, a vulnerable important woody liana with high medicinal value and limited reproductive capacity has been over exploited due to its demand in the pharmaceutical industry. In the present study, an efficient micropropagation protocol was developed from nodal explants on Murashige and Skoog and woody plant medium. The effect of various strengths of WP and MS medium (total, half strength) and different concentrations of plant growth regulators including cytokinins [6-benzylaminopurine (BAP) and Kinetin) and auxins (α-naphthaleneacetic acid (NAA) and (IBA) indole-3-butyric acid] on proliferation and rooting was investigated. C. paniculatus shoots showed maximum proliferation in WP medium supplemented with BAP (0.5 mg l-1). Best rooting frequency and higher root number and length were attained in medium fortified with NAA in combination with 0.2% activated charcoal. Seventy percent of the plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development. These plantlets can be used to replenish the declining populations in the wild. Molecular profiling of the in vitro developed plantlets was done using RAPD to confirm their genetic uniformity.
Characterization of IcaB protein Poly-N-acetylglucosamine (dPNAG) De-N-acetylase involved in Staphylococcus epidermidis biofilm formation
Authors: Prabu Ramachandira , Veeramani T.
Published: June 2023

Quorum sensing (QS) is type of behaviour coordinating bacterial cell-cell interaction involved in EPS production, cell motility, toxin secretion, biofilm. Biofilms forming multidrug-resistant Staphylococcus epidermidis (MRSE) are major threats to public health undergoing slime formation and bacteremia. Biofilms causes nosocomial infection, chronic skin damage, vaginal wall inflammation, gut infection, endocardium infection and stomach infection. One of the serious concern is biofilm formation, which helps to prevent antibiotics diffusion leading to decrease in bacterial metabolic activity mechanism. Methicillin-resistant biofilm forming S. epidermidis, RP62A (ATCC 35984) isolate is slime-producing isolate. RP62A isolates has been well characterized from molecular and infection studies revealing that plasmid (pEpi62) carries antibiotics resistance genes to penicillin, kanamycin, erythromycin and streptomycin. De-N-acetylation of poly-N-acetylglucosamine (dPNAG) is an extracellular exopolysaccharide for biofilm development. We have characterized IcaB gene and protein sequence involved in Staphylococcus epidermidis biofilm formation.
Pharmacological studies of Selenium Nanoparticles synthesized from Syzygium aromaticum
Authors: Hemalatha Senthilkumar , Hariprasath Lakshmanan , Nishu Sekar , Selvakumar Dharmaraj
Published: June 2023

Selenium is an essential element and if taken in trace amounts, then it plays an important role in plant and animal development and reproduction. When compared to other methods of selenium nanoparticles (SeNPs) production, green synthesis using plant extracts has a better probability of eliminating its harmful character and producing beneficial SeNPs. The current work used Syzygium aromaticum to perform biogenic synthesis of SeNPs. The biosynthesized SeNPs were characterized by UV-Vis spectroscopy, X-Ray Diffraction, FTIR, AAS, SEM-EDX and FESEM. The biosynthesized SeNPs showed maximum peak at 276 nm and FESEM image exhibited greater numbers of the agglomerated spherical particles within the diameter range 30-55 nm. The antioxidant activity was measured in vitro using the radical scavenging (DPPH) test, total antioxidant (FRAP) assay and intracellular reactive oxygen species (ROS) assay against the A375 cell line. The formation of free radicals of selenium elements as antioxidant qualities prevented the growth of A375 skin cancer cells. In vitro tests revealed that SeNPs had the highest percentage of inhibition. As a consequence, the current study's findings showed that the lowest concentrations of SeNPs had better antioxidant effects. MTT experiment was used to assess the cytotoxicity of SeNPs against A375 cell lines exhibiting an IC50 value of 27±1.2 g/ mL. Synthesized SeNPs showed less toxicity towards the investigated cell line which remained more than 50% viability at 48 hours of exposure. In vitro anthelmintic activity data demonstrate that the worms experienced paralysis and death time at 25.03± 0.12 and 60.12±1.90 minutes respectively. These SeNPs with diverse objectives may exhibit increased absorption by functionalization and simultaneous drug loading as well as agnostic characteristics in the future.
Atherogenic Index of Plasma and Oxidized-LDL as Strong and Independent Predictor of CHD in Young Smokers
Authors: Nath Dinesh , Shivasekar Meera , Vinodhini V.M.
Published: June 2023

Cigarette smoking is an environmental risk factor for cardiovascular disease and induces changes in lipid profile by the oxidation of lipids, particularly LDL. Hence the need for determine ox-LDL and its association with atherogenic indices in the prediction of cardiovascular risk arises. The study aimed to determine oxidized-LDL and atherogenic indices in relation to smoking habits in cigarette smokers. This case-control study included 240 individuals in which cigarette smokers were (n=120) and were compared with (n=120) non-smokers. The ox-LDL, APO-A1 and APO-B were performed using Enzyme-linked Immune Sorbent Assay (ELISA) and atherogenic risk ratios were calculated. The study shows that ox-LDL was significantly increased in smokers and significant increase in TC, TGL and LDL was also observed. When compared to nonsmokers, smokers had considerably higher atherogenic risk indices. Pearson correlation tests revealed that ox-LDL and AIP were substantially and positively linked with non-HDL, CRI- Castelli's risk index-I, CRII- Castelli's risk index-II, AC-Atherogenic coefficient, CI-Cholindex and APO-B- Apolipoprotein B (p<0.001). The unadjusted odds ratios for ox-LDL and AIP in the univariate logistic regression analysis were 1.861 (95% confidence interval: 1.347-2.375), p<0.001 and 1.782 (95% confidence interval: 1.490-2.131), P.001) respectively. Multivariate logistic regression analysis demonstrated that ox-LDL and AIP were independent risk factors for CHD in young smokers. In smokers, ox-LDL and atherogenic indices appear to be strong predictors of CHD than conventional lipid parameters.
Anticancer and antimicrobial activity of three different seaweed extracts
Authors: Annepu Ravi Kumar , Prattipati Subhashini Devi
Published: June 2023

In the present study, antimicrobial and in vitro cytotoxic activities of three seaweeds Gracilaria corticata (red algae), Ulva lactuca (green algae) and Sargassum ilicifolium (brown algae) were reported. Among the three algal extracts screened for antibacterial activity, the acetone extract of all the three algae showed moderate antibacterial activity on Gram positive bacteria S. aureus and B. subtilis and Gram negative bacteria E. coli and P. aeruginosa but no activity was observed on M. luteus and S. typhi. The cytotoxic activity of three seaweeds was studied using MTT assay on MCF-7 cell lines. G.corticata showed highest inhibition percent (51.13±0.25) when compared to U. lactuca (35.63±0.70) and S. ilicifolium (31.56±0.54). Among the three solvents tested, methanol was found to be the most suitable solvent for the extraction of compounds.
Assessment of biological properties for decellularized human umbilical cord tissue: Biomaterials in regenerative medicine
Authors: Muthuraman Muthuchamy , Kumaran Subramanian , Chirayu Padhiar , Dhanraj Arvind Kumar , Desireddy Swathi
Published: June 2023

Human placental tissues are used as raw materials for applications in regenerative medicine. The extracellular matrix scaffolds which are abundant in collagen, glycosaminoglycans, proteoglycans, growth factors and cytokines are effective in accelerating the healing process. In this study, the processed umbilical cord was assessed for morphological and functional characteristics to assess its competence for regenerative properties in wound healing. The histological analysis of the umbilical cord was assessed with hematoxylin and eosin and immunohistochemistry for type-IV collagen. Physical properties such as ball burst, thickness and moisture were examined. ELISA and multiplex assays were performed to estimate the growth factors and cytokines. Anti-inflammatory properties were assessed using RAW 264.7 cell line. Further, functional aspects were determined through cell proliferation, cell migration and tube formation studies. Histological results revealed the scaffold had retained its structural features like an amnion layer with epithelial cells and sparsely distributed stromal cells in Wharton jelly. Physical properties were consistently maintained across the tissues. Functional analysis showed reduced macrophage proliferation, increased cell proliferation and cell migration validating its efficacy towards wound healing. Altogether data suggest that the umbilical cord is an effective remedial biological cover for wound healing applications with therapeutic potential for tissue repair and regeneration.
Assessment of microbiological isolates from semen in men attending an infertility clinic in Meghalaya
Authors: Hynniewta Barry Cooper , Phanjom Probin , Kurbah Ronald Eleazer , Mynso Kapil Slong
Published: June 2023

Married couples all over the world face varying degrees of infertility. Semen analysis is the first and most important laboratory investigation of the male partner of an infertile couple. Infectious agents such as viruses, bacteria, fungi and protozoa cause urogenital infections which account for about 15% of male infertility concerns. In this study, we explored the effect of bacterial infections in male factor infertility in an infertility clinic, Meghalaya, India by detecting abnormal sperms and other factors. Two hundred men's seminal fluid was examined for infertility using the Makler counting chamber and microbiological culture techniques to detect aberrant sperm and isolate bacteria respectively. 117 (58.5%) of 200 infertile patients tested positive for pathogenic bacteria. Average sperm motility in uninfected semen was 47.14 % compared to 34.21 % in infected samples. Enterococcus faecalis was most prevalent (26%) followed by Escherichia coli (15%). Bacterial infection is associated with detrimental impacts on various semen parameters. Semen microbiological culture is an underrated tool, not requested by many IVF specialists prior to fertility treatment. Antibiotic treatment needs to be administered to both husband and wife to avoid re-cross infection.
Acute dermal toxicity study of flower essential oil from Etlingera fenzlii (Kurz.) K. Schum. in Wistar albino rats
Authors: Anand Aswathy A. , Felix Shibin P. , Mohan Maya , Shan Sasidharan , Radha R.K.
Published: June 2023

WHO has emphasized the value of using green repellents to combat disease and supplement insecticide-based vector control strategies. These products are frequently labelled as safe which is a common misperception due to the presence of varying bioactive compounds that can either be toxic or non-toxic. Pertaining to this scenario, a scarcely studied plant Etlingera fenzlii is studied to analyse its toxicological effects on skin. The plant is endemic to Kamorta and Katchal islands of Nicobar group and Mount Harriet hill ranges of Southern Andaman. These plants are used by the Shompen tribes for their honey collection and also against stomach disorders, cough etc. It is crucial to comprehend the plant's safety levels and dose limitations considering its' proven fungicidal and mosquitocidal characteristics among their other functions. The current study evaluated the acute dermal toxicity of flower essential oil in Wistar rats in accordance with OECD recommendations. Topical application at a dose of 2000mg/kg body weight showed no signs of toxicity. Any changes in body weight, water intake, feed consumption or gross pathology were not observed in the tested animals which provide considerable evidence to support the safe topical application of the essential oil.
Preliminary Phytochemical Analysis and Screening of Environmental Influence on Constituents of Saptaparni Leaves
Authors: Palshikar Gautam , Shanmugapandiyan Pitchaimuthu
Published: June 2023

Herbs act as rich source of active phytoconstituents and are used in Ayurvedic and allopathic dosage forms to provide new remedies for treatment of human disorders. Saptaparni, an Indian Devil Tree, Chhatim, Alstonia scholaris (L.) R. Br. is highly potent herb in traditional Indian Ayurvedic medicinal system. Today more population gets suffered from diabetes, an endocrine and metabolic disorder. Herbal medicines are used for treatment of such chronic diseases from hundreds of years. Variations due to an external environmental condition have impact on potency of active components in herbs which also influence therapeutic efficacy. For getting yield with more potency, the harvesting should be done properly at particular and specific conditions. Research objective is to evaluate alterations in erythrodiol content of leaves of an Indian devil tree, Alstonia scholaris. Plant material was collected in different seasons, time and places and then identification and analysis were done. Morphological, microscopical and extractive values show differences. HPTLC analysis shows that concentration of erythrodiol was found to be higher from the ethanol extract obtained in the February- May in warm atmospheric conditions and at high altitude place.
Isolation and Molecular Identification of Cellulase producing bacteria from soil and litter in Veerakkal forest area, The Western Ghats, India
Authors: Ramya E.K. , Sharmila S. , Mownika S. , Abdul K. , Ann Suji H.
Published: June 2023

The cellulolytic bacterial strains were screened from both soil and litter samples in different study sites of Veerakkal forest, Manar beat, The Western Ghats, India. In the current investigation, out of 64 and 48 differentiated total purified isolates, 53 soil isolates and 34 litter isolates were screened based on the CMC method. The percent wise calculation indicated that 82 % were cellulase positive while 18 % were negative in soil isolates. Likewise, 70 % of them were positive while 30 % were negative cellulase strains in litter isolates. The average hydrolysis capacity (HC) values ranged between 1.67 to 3.25 cm. The highly potential cellulolytic bacterial isolates viz. Bacillus thuringiensis, Bacillus licheniformis, Bacillus cereus, Bacillus sporothermodurans, Bacillus circulans, Bacillus albus, Bacillus amyloliquefaciens, Lysinibacillus sphaericus and Bacillus mobilis were identified from soil. Likewise, the litter cellulolytic bacterial species such as Bacillus sp., Lysinibacillus macroides, Bacillus paramycoides, Solibacillus silvestris, Pseudomonas stutzeri, Bacillus toyonensis, Bacillus subtilis, Bacillus pacificus, Bacillus flexus, Bacillus paranthracis and Pseudomonas putida were identified. The cellulolytic bacterial population was higher during the monsoon because of the presence of higher moisture content in leaf litter. However, the cellulolytic bacterial population of soil was higher during the pre-monsoon season because of the continuous breakdown of litter which inhaled more cellulolytic strains in the soil. Finally, it was concluded that corresponding with seasonal changes, the cellulolytic bacterial community also changed and it plays a significant role in the mineralization process.