bluuebunny/crossref_metadata_2025 huggingface dataset:
Neuroprotective Effects of Activated Protein C Involve the PARP/AIF Pathway against Oxygen-Glucose Deprivation in SH-SY5Y Cells
Authors: Sriwastva Mukesh Kumar , Kunjunni Remesh , Andrabi Mutahar , Prasad Kameshwar , Saxena Renu , Subbiah Vivekanandhan
Published: December 2020

Protein C, a member of the zymogen family of serine proteases in plasma, is one of the several vitamin K dependent glycoproteins known to induce anti-apoptotic activity. However, the target molecule involved in the mechanism needs to be investigated. We sought to investigate the pathways involved in the anti-apoptotic role of activated protein C (APC) on oxygen-glucose deprivation (OGD) induced ischemic conditions in in-vitro SH-SY5Y cells. SH-SY5Y cells were exposed to OGD in an airtight chamber containing 95% N2 and 5% CO2 and media deprived of glucose for 4 h following 24 h of reoxygenation. The cell toxicity, viability, expression of receptors such as endothelial cell protein C receptor (EPCR), protease-activated receptor (PAR)1, PAR3, and apoptosis-related proteins B-cell lymphoma 2 (BCL-2), BCL-2-like protein 4 (Bax), Poly [ADP-ribose] polymerase-1 (PARP-1) were assessed. Administration of APC decreased the cellular injury when compared to the OGD exposed group in a dose-dependent manner and displayed increased expression of PAR-1, PAR-3, and EPCR. The APC treatment leads to a reduction in PARP-1 expression and cleavage and apoptosis-inducing factor (AIF) expression. The reduction of caspase-3 activity and PARP-1 and AIF expression following APC administration results in restoring mitochondrial function with decreased cellular injury and apoptosis. Our results suggested that APC has potent protective effects against in-vitro ischemia in SH-SY5Y cells by modulating mitochondrial function.
Some Remarks on Colloid Stability: Selected Examples Taken from the Milk Chain for Food Prepares
Authors: La Mesa Camillo , Risuleo Gianfranco
Published: December 2020

Different forces play key roles in the stability of food colloid dispersions. The focus here is on those controlling attraction and/or repulsion, which concur to stabilization, phase separation, coagulation and are quite evident in water-based systems. The combination of attractive and repulsive forces favors or hinders the association of colloid entities; such processes are often met in food technology. The above processes depend on the forces at work and colloid concentration in the medium (i.e., on interparticle distance). Worked examples deal with milk manipulation procedures, ending in cheese formation. The whole milk sequence is controlled by the combination of forces leading to aggregation and phase separation of casein and other milk components. Thereafter, one gets either fresh, for prompt consumption, or aged cheeses. The combination of attractive (van der Waals, vdW, and depletion) with repulsive (double layer, DL, but also steric) forces results in the dominance of aggregation versus dispersion modes in the milk transformation chain, which depends on the distance among colloid particles, on the amplitude of the mentioned forces, and on their decay. The combined role of double layer and van der Waals (vdW) forces is at the basis of the DLVO theory on colloid stability, which is properly modified when these forces overlap with steric stabilization and, eventually, with depletion. Steric effects are dispersive, and depletion ones favor colloid nucleation in a single phase. The milk manipulation chain is a worked example of the intriguing association features controlled by the mentioned forces (and of ancillary ones, as well), and indicates which forces favor the formation of products such as parmesan or mozzarella cheese but are not alien to the preparation of many other dairy products.
The Influence of Signal Polarization on Quantum Bit Error Rate for Subcarrier Wave Quantum Key Distribution Protocol
Authors: Gaidash Andrei , Kozubov Anton , Medvedeva Svetlana , Miroshnichenko George
Published: December 2020

In this paper, we consider the influence of a divergence of polarization of a quantum signal transmitted through an optical fiber channel on the quantum bit error rate of the subcarrier wave quantum key distribution protocol. Firstly, we investigate the dependence of the optical power of the signal on the modulation indices’ difference after the second phase modulation of the signal. Then we consider the Liouville equation with regard to relaxation in order to develop expressions of the dynamics of the Stokes parameters. As a result, we propose a model that describes quantum bit error rate for the subcarrier wave quantum key distribution depending on the characteristics of the optical fiber. Finally, we propose several methods for minimizing quantum bit error rate.
Towards Ferry Electrification in the Maritime Sector
Authors: Anwar Sadia , Zia Muhammad Yousuf Irfan , Rashid Muhammad , Rubens Gerardo Zarazua de , Enevoldsen Peter
Published: December 2020

The electrification of vessels/ferries for green transformation requires onboard electrical energy storage as well as an energy supply network in the port area. In this context, a lot of efforts have been made in the last decade that have been reviewed in such a way that only a single aspect of the green transformation challenge is highlighted. Consequently, the objective of this research is to develop knowledge by examining the current state of affairs and provide, accordingly, abstract implementation guidelines for green transformation through vessel/ferry electrification. A comprehensive study on the electrification of vessels, in industry as well as in academia, is performed. Based on the data collected through a systematic study, a comparison of various pure electric and hybrid vessels in terms of certain performance attributes, such as battery capacity, passenger and cargo capacities, and size (length) of the vessel, is performed. Moreover, the distribution of vessels according to different countries and manufacturers is provided. Finally, certain technical, operational, and legislative challenges are explored.
Surfactant-Polymer Interactions in a Combined Enhanced Oil Recovery Flooding
Authors: Druetta Pablo , Picchioni Francesco
Published: December 2020

The traditional Enhanced Oil Recovery (EOR) processes allow improving the performance of mature oilfields after waterflooding projects. Chemical EOR processes modify different physical properties of the fluids and/or the rock in order to mobilize the oil that remains trapped. Furthermore, combined processes have been proposed to improve the performance, using the properties and synergy of the chemical agents. This paper presents a novel simulator developed for a combined surfactant/polymer flooding in EOR processes. It studies the flow of a two-phase, five-component system (aqueous and organic phases with water, petroleum, surfactant, polymer and salt) in porous media. Polymer and surfactant together affect each other’s interfacial and rheological properties as well as the adsorption rates. This is known in the industry as Surfactant-Polymer Interaction (SPI). The simulations showed that optimum results occur when both chemical agents are injected overlapped, with the polymer in the first place. This procedure decreases the surfactant’s adsorption rates, rendering higher recovery factors. The presence of the salt as fifth component slightly modifies the adsorption rates of both polymer and surfactant, but its influence on the phase behavior allows increasing the surfactant’s sweep efficiency.
Technical and Economic Analysis of the External Surface Heating System on the Example of a Car Park
Authors: Nowak Krzysztof , Rabczak Sławomir
Published: December 2020

In winter, for our own safety, as well as for the comfort of the user, we are obliged to remove or mitigate the defects related to the accumulation of snow and ice on flat surfaces, such as: pavements, stairs, driveways, parking lots, roofs, squares, or sports fields. Snow and ice from these surfaces can be removed by a variety of methods. Chemical, mechanical, or heating methods are most often used. Mechanical and manual methods cannot always be used. They also often do not allow the complete removal of snow and ice from the surface. In chemical methods, the chemicals used can have a negative impact on the environment and the surface itself. Heating external surfaces using electric heating cables or liquid-filled pipes is one of the safest and most effective ways to remove snow and ice from the available methods. The article presents a technical concept of a car park heating system with the use of various heating systems. The main thesis of the work is the possibility of using heating systems to maintain the quality of external parking spaces in winter. The authors tried to prove that it is possible to use a number of heating systems based on commonly known energy carriers for this purpose. The concept was made for the conditions prevailing in Poland. The systems were compared in financial and ecological terms. The following systems were analyzed: electric heating, heating with the use of a heat pump with a vertical ground heat exchanger, and liquid heating with various heat sources (including heat from the district heating network, hard coal boiler, biomass boiler, fuel oil boiler, natural gas boiler). From a cognitive point of view, it was interesting to examine whether the proposed installation with a heat pump is technically feasible and economically and ecologically justified.
Germination and Seedling Growth Responses of Zygophyllum fabago, Salsola kali L. and Atriplex canescens to PEG-Induced Drought Stress
Authors: Reza Yousefi Ali , Rashidi Sakineh , Moradi Parviz , Mastinu Andrea
Published: December 2020

In arid and semi-arid regions, planting drought-tolerant species is the most useful strategy in the reclamation of degraded soils. In the present study, we evaluated the effect of simulated drought by polyethylene glycol (PEG-6000) on seed germination and seedling growth of three desert plants such as Atriplex canescens, Salsola kali and Zygophyllum fabago. Seeds were subjected to water stress to drought stress by PEG at five stress levels (0, −1, −4, −8, −12, −14 bars). Germination of Z. fabago was completely inhibited at an osmotic potential of −8, −10 and −12 bars and the germination of A. canescens was inhibited only at −14 bar. In contrast, S. kali responded positively to high levels of stress and our results showed the highest final germination percent (71.75, 54 and 18.25%) under three-drought stress −8, −12 and −14 bars, respectively. In addition, increasing PEG concentration adversely affected the germination rate and seedling vigor index as well as the root and shoot length of species. Under high stress levels, S. kali achieved a higher germination rate and seedling vigor index compared to Z. fabago and A. canescens. Among species, S. kali was the only one able to develop roots and shoots at −14 bar. Therefore, S. kali could be considered as a promising plant for the rehabilitation of degraded soils at risk of desertification.
Free Flow and Discharge Characteristics of Trapezoidal-Shaped Weirs
Authors: Zerihun Yebegaeshet T.
Published: December 2020

A number of studies have considered the effects of weir design variations on the free- and submerged-flow characteristics of trapezoidal broad-crested weirs. It appears that the hydraulics of short-crested weir flows have received little attention; thus, the current knowledge is incomplete. By systematically analyzing a large set of experimental data, the present study aims to fill in this knowledge gap and to provide a complete description of the discharge characteristics of trapezoidal-shaped weirs, including the salient features of two-dimensional weir flows. The analysis of the axial free-surface profiles for short-crested weir flows attested that the location of the nearest station for the correct measurement of the overflow depth under free-flow conditions is at η0  from the heel of the weir, where η0  is the upstream free-surface elevation. Additionally, an empirical equation for the free-flow discharge coefficient is proposed as being valid for a trapezoidal-shaped weir with varying upstream- and downstream-face slopes. The results of this investigation reveal that the streamline curvature and the slopes of the upstream and downstream weir faces significantly affect the streamwise flow patterns and, hence, the free-flow discharge.
Healing Is Rhizomatic: A Conceptual Framework and Tool
Authors: Lopez Jennifer
Published: December 2020

This paper offers a conceptual framework and a set of tools that use rhizomes as a metaphor for healing in the context of oppressive violence. Existing conceptualizations of trauma, trauma recovery, and healing offer important tools for framing and addressing the impacts of oppression on individuals and groups. These exist in a fractured practice ground where practitioners are socialized into divisions such as “micro” vs. “macro” practice and “self-care” vs. “the work.” The Healing is Rhizomatic conceptual framework identifies five nodes (body, felt sense, relationships, place, story) and three dimensions of healing-oriented engagement (recognition, readying the ground, (re)generation) that exist across these approaches. Adaptable to multiple levels of analysis, the conceptual framework focuses on fracture, blockage, and connection as core experiences. These experiences occur in, through, and between the nodes and dimensions. This paper explores use of the conceptual framework and tools for applying it as a mechanism for assessment and reflection about internalized and operational definitions and approaches to healing. Thereby, the framework and tools offer a view of a common practice ground for practitioners engaged in healing work in the context of oppressive violence and is intended to support deeper awareness, collaboration, and coordination of approaches.
Interrelation of Ecophysiological and Morpho-Agronomic Parameters in Low Altitude Evaluation of Selected Ecotypes of Sweet Potato (Ipomoea batatas [L.] Lam.)
Authors: Burbano-Erazo Esteban , Cordero Carina , Pastrana Iván , Espitia Laura , Gomez Evelin , Morales Alfredo , Pérez Jazmín , López Leonardo , Rosero Amparo
Published: December 2020

Sweet potato is a crop with a wide capacity to adapt to adverse conditions. To study the tolerance of the sweet potato to a low-altitude environment, 34 genotypes comprising three groups from different altitude conditions ranging from 18–599, 924–1298, 1401–2555 meters above sea level were evaluated. These genotypes were evaluated through ecophysiological parameters: net photosintetic rate (Pn), stomatal conductance (GS), transpiration (E), leaf internal CO2 (ICO2), vapor pressure deficit (VPD) and leaf internal temperate (LT). sSubsequently, water use efficiency (WUE) and carboxylation efficiency index (CEI) were estimated. Simultaneously, morpho-agronomic characterization of the genotypes was conducted including descriptors and morpho-colorimetric parameters. A wide ecophysiological variability was found among genotypes from high, intermediate and low altitudes, when those were evaluated under low altitude conditions. The genotypes that presented major soil coverage efficiency and leaf size showed greater Pn, WUE and CEI, and Low VPD and E, aspects that benefited the ability to form roots the under low-altitude environment. The altitudinal origin of the genotypes influenced the ecophysiological response under low altitude conditions. The capacity of certain sweet potato genotypes to tolerate low altitude conditions were due to to different mechanisms, such as certain morphoagronomic traits that allowed them to adjust their physiological processes, especially those related to photosynthesis.
Metal Fractionation in Surface Sediments of the Brahmaputra River and Implications for Their Mobilization
Authors: Tsering Tenzin , Sillanpää Mika , Reinikainen Satu-Pia , Abdel Wahed Mahmoud
Published: December 2020

The Brahmaputra River is the largest tropical river in India that flows along the Himalayan regions and it is the lifeline of millions of people. Metal fractionation in the Brahmaputra River’s surface sediments and its correlation with turbidity are assessed in this study. The interaction between metal fractions and the overlying water is studied using multivariate statistical analyses. The strong positive correlation between NH4 of the overlying water and the exchangeable fractions in sediments signifies that the metals in the exchangeable fractions can be substituted by NH4. Subsequently, these metals can be released into the overlying water. The fluctuation in turbidity from 73 to 875 NTU indicates a large variation in the suspended matter concentration, and a higher concentration of suspended matter could provide attachment sites for pollutants such as metals. Significant variation in turbidity manifests a potentially high risk of pollution. In addition, the observation of local people along the Brahmaputra River turning its color to muddy indicates the need for continuous monitoring of water quality and an assessment of pollution is crucial. Although the Brahmaputra River’s risk assessment code is at low risk, the exchangeable fractions of Ni and Zn are present at all sites. Thus, the Brahmaputra River requires early preventive measures and management strategies to control metal pollution. This study contributes to an understanding of the fluctuation of turbidity of a tropical river. We provide baseline data for policymakers, and the importance of further intensive studies on metal pollution in the Himalayan Rivers is highlighted.
Effects of Aerobic and Resistance Exercise Interventions on Cognitive and Physiologic Adaptations for Older Adults with Mild Cognitive Impairment: A Systematic Review and Meta-Analysis of Randomized Control Trials
Authors: Lee Junga
Published: December 2020

(1) Background: The purpose of this meta-analysis is to investigate the effects of exercise intervention for older adults with mild cognitive impairment (MCI). (2) Methods: Databases including PubMed, Medline, and Cochrane were used to search for studies that reported effects of exercise for older adults with MCI and randomized controlled trials up to July 2020. Exercise interventions of all selected studies were summarized, and effect sizes of exercise interventions were calculated. (3) Results: A total of 14 studies, including 1178 older adults with MCI were included. Exercise participation in older adults with MCI improved cognitive functions (d = 0.88, 95% confidence interval [CI]; 0.10–1.65, p = 0.01; k = 5]) and handgrip strength (d = 0.62, 95% CI; 0.23–1.01, p = 0.00; k = 4) compared with control groups. Aerobic exercise or resistance exercise at moderate to vigorous levels for at least 150 min, 1 time/week, for 6 weeks was the minimum level to obtain beneficial effects from exercise for older adults with MCI. (4) Conclusions: Older adults with MCI who participated in exercise received beneficial effects, including improvement in cognitive functions and handgrip strength, but further studies to confirm the effects are needed.
Promotion of Disposable Electronic Cigarette Flavors and Topics on Twitter
Authors: Sun Li , Tao Chunliang , Xie Zidian , Li Dongmei
Published: December 2020

Disposable electronic cigarettes (e-cigarettes) became popular among youth after the Food and Drug Administration (FDA) implemented an enforcement policy to restrict the sale of cartridge-based flavored e-cigarettes starting from February 2020 in the United States (US). We aimed to examine the flavors and topics related to disposable e-cigarettes on Twitter. The Twitter dataset, which includes 1489 tweets, was collected by the Tweepy streamapplication programming interface (API) using a keyword query from March to September 2020. The disposable e-cigarette flavors were curated from both online stores and collected tweets. Topics related to disposable e-cigarettes on Twitter were manually coded. Distributions of topics were compared between tweets from the US and tweets from non-US countries. The temporal analysis results showed a slight increase in the number of discussions over the study period. Strawberry, mango, watermelon, and mint were the most popular flavors of disposable e-cigarettes mentioned on Twitter. Almost all the tweets (97.11%) were commercial tweets, which were dominated by topics related to the product and flavor promotions. The US tweets focused more on product and flavor promotions and less on price promotions compared to non-US tweets. Our results suggest that companies exploited the limitations of legislation to promote flavors on Twitter, which could undermine public health and young people’s finances if they get hooked on addictive products.
A Multimodal Stress-Prevention Program Supplemented by Telephone-Coaching Sessions to Reduce Perceived Stress among German Farmers: Results from a Randomized Controlled Trial
Authors: Stier-Jarmer Marita , Oberhauser Cornelia , Frisch Dieter , Berberich Götz , Loew Thomas , Schels-Klemens Carina , Braun Birgit , Schuh Angela
Published: December 2020

This study compared the effectiveness of a 12-day stress-prevention program (SGS) supplemented by individualized, structured, four-session telephone-coaching to that of an SGS without telephone-coaching in entrepreneurs from the green professions presenting with increased stress levels. All participants went through the SGS before being randomized either to the telephone-coaching group (TC) or to the control group without telephone-coaching (noTC). SGS included four key therapeutic elements: stress-management intervention, relaxation, physical exercise, and balneotherapy. The primary outcome was the current degree of subjectively experienced stress assessed with the Perceived Stress Questionnaire (PSQ) at a 9-month follow-up. Secondary outcomes included burnout symptoms, well-being, health status, sleep disorders, expectation of self-efficacy, depression, anxiety, ability to work, pain, and days of sick leave. Assessments were conducted at baseline, 12 days (end of program), and 1 (start telephone-coaching), 3, 6 (end of telephone-coaching), and 9 months. Data from 103 adults (TC = 51; noTC = 52), mostly fulltime farmers, were available for analysis (mean age: 55.3; 49.1% female). Participants experienced significant immediate improvement in all outcome measurements, which declined somewhat during the first three months after the end of SGS and then remained stable for at least another six months. While within-group changes from baseline to 9 months showed significant improvements at medium to large effect sizes for all target variables (PSQ-total, TC: −13.38 (±14.98); 95%-CI: (−17.68; −9.07); noTC: −11.09 (±14.15); 95%-CI: (−15.11; −7.07)), no statistically significant differences were found between the groups at any time and for any target variable (between-group ANCOVA for PSQ-total at 9 months, parameter estimator for the group: −1.58; 95%-CI: (−7.29; 4.13)). The stress-prevention program SGS is a feasible, effective, and practical way to reduce perceived stress and improve participants’ resources. Four subsequent telephone-coaching sessions do not seem to contribute to a further improvement in the results.
Geriatric Nutritional Risk Index as a Tool to Evaluate Impact of Malnutrition Risk on Mortality in Adult Patients with Polytrauma
Authors: Yeh Cheng-Hsi , Wu Shao-Chun , Chou Sheng-En , Su Wei-Ti , Tsai Ching-Hua , Li Chi , Hsu Shiun-Yuan , Hsieh Ching-Hua
Published: December 2020

Background: Identification of malnutrition is especially important in severely injured patients, in whom hypermetabolism and protein catabolism following traumatic injury worsen their nutritional condition. The geriatric nutritional risk index (GNRI), based on serum albumin level and the current body weight/ideal body weight ratio, is useful for identifying patients with malnutrition in many clinical conditions. This study aimed to explore the association between admission GNRI and mortality outcomes of adult patients with polytrauma. Methods: From 1 January 2009 to 31 December 2019, a total of 348 adult patients with polytrauma, registered in the trauma database of a level I trauma center, were recognized and categorized into groups of death (n = 71) or survival (n = 277) and into four nutritional risk groups: a high-risk group (GNRI < 82, n = 87), a moderate-risk group (GNRI 82 to <92, n = 144), a low-risk group (GNRI 92–98, n = 59), and a no-risk group (GNRI > 98, n = 58). Univariate and multivariate logistic regression analyses were used to identify the independent risk factors for mortality. The mortality outcomes of patients at various nutritional risks were compared to those of patients in the no-risk group. Results: The comparison between the death group (n = 71) and the survival group (n = 277) revealed that there was no significant difference in gender predominance, age, pre-existing comorbidities, injury mechanism, systolic blood pressure, and respiratory rate upon arrival at the emergency room. A significantly lower GNRI and Glasgow Coma Scale score but higher injury severity score (ISS) was observed in the death group than in the survival group. Multivariate logistic regression analysis revealed that Glasgow Coma Scale (GCS), odds ratio (OR), 0.88; 95% confidence interval (CI), 0.83–0.95; p < 0.001), ISS (OR, 1.07; 95% CI, 1.04–1.11; p < 0.001), and GNRI (OR, 0.94; 95% CI, 0.91–0.97; p < 0.001) were significant independent risk factors for mortality in these patients. The mortality rates for the high-risk, moderate-risk, low-risk, and no-risk groups were 34.5%, 20.1%, 8.5%, and 12.1%, respectively. Unlike patients in the moderate-risk and low-risk groups, patients in the high-risk group had a significantly higher death rate than that of those in the no-risk group. Conclusions: This study revealed that the GNRI may serve as a simple, promising screening tool to identify the high risk of malnutrition for mortality in adult patients with polytrauma.
A New Methodology to Design Sustainable Archimedean Screw Turbines as Green Energy Generators
Authors: Alonso-Martinez Mar , Suárez Sierra José Luis , Coz Díaz Juan José del , Martinez-Martinez Juan Enrique
Published: December 2020

Current energy demand and climate target plans are leading to green energy facilities which are efficient and sustainable. Archimedean screw turbines (ASTs) are used to generate hydroelectricity in low heads. They have been manufactured and installed worldwide. However, there is a lack of knowledge about how to design them efficiently. In this study, the performance of ASTs is analyzed using an analogy between ASTs and bucket elevators. Based on this analogy, a theoretical hypothesis on how to produce efficient ASTs is proposed. The new methodology for the design of ASTs is based on two considerations: the filling level of the AST buckets must be 85% and the increase of leakage losses must be minimized. This hypothesis is numerically and experimentally studied. Two experimental prototypes were developed and installed in the north of Spain. The numerical and experimental results are provided. A discussion comparing the results of this work and other results from the literature is presented. Finally, conclusions are drawn from this work that contribute to the improvement of AST technology as a sustainable facility to generate green energy.
Risk and Protective Factors of Disordered Eating in Adolescents Based on Gender and Body Mass Index
Authors: Argyrides Marios , Anastasiades Elly , Alexiou Evangelia
Published: December 2020

The current study aimed to identify potential psychosocial risk and protective factors contributing to eating disorders in adolescents, and observe any differences between genders and Body Mass Index (BMI) categories. A cross-sectional survey was carried out with a total of 2605 (1063 male) adolescents, who were assessed for disordered eating, body-image satisfaction and investment, appearance/weight-related anxiety, situational dysphoria, media influences, self-esteem, and body appreciation. The results revealed that weight/appearance-related anxiety and situational dysphoria were the most significant risk factors for both genders. Pressures from the media posed a significant risk only for males and the internalization of the thin ideal only for females; however, the internalization of the athletic ideal did not pose as a significant risk factor. Compared to gender, these risk factors did not differ based on BMI. Additionally, body appreciation was found to be a robust protective factor (unlike global self-esteem) for both genders, and across all BMI groups. The findings indicate that the most significant risk and protective factors of eating disorders do not differ largely for male and female adolescents or different BMIs. Intervention and prevention programs would therefore benefit from the inclusion of exercises that reduce the constructs of weight/appearance-related anxiety and situational dysphoria, and promote body appreciation.
What Is the Consensus from Multiple Conclusions of Future Crop Yield Changes Affected by Climate Change in China?
Authors: Huang Chengfang , Li Ning , Zhang Zhengtao , Liu Yuan , Chen Xi , Wang Fang , Chen Qiong
Published: December 2020

Many studies have shown that climate change has a significant impact on crop yield in China, while results have varied due to uncertain factors. This study has drawn a highly consistent consensus from the scientific evidence based on numerous existing studies. By a highly rational systematic review methodology, we obtained 737 result samples with the theme of climate change affecting China’s crop yields. Then, we used likelihood scale and trend analysis methods to quantify the consensus level and uncertainty interval of these samples. The results showed that: (i) The crop yield decrease in the second half of the 21st century will be greater than 5% of that in the first half. (ii) The crop most affected by climate change will be maize, with the decreased value exceeding −25% at the end of this century, followed by rice and wheat exceeding −10% and −5%. (iii) The positive impact of CO2 on crop yield will change by nearly 10%. Our conclusions clarify the consensus of the impact of future climate change on China’s crop yield, and this study helps exclude the differences and examine the policies and actions that China has taken and should take in response to climate change.
Evaluation of the Efficiency of Basic Sanitation Integrated Management in Brazilian Municipalities
Authors: Cavalcanti Alvaro , Teixeira Arthur , Pontes Karen
Published: December 2020

This study aims to evaluate the level of technical efficiency of companies that perform the integrated management of basic sanitation in Brazilian municipalities. A Multiple Data Envelopment Analysis (M-DEA) model was applied to estimate the performance of water supply and sewage services in 1628 municipalities covering more than 56% of the Brazilian population, identifying the factors that most influence the efficiency of the sector in the years 2008 and 2016. The M-DEA methodology is an extension of Data Envelopment Analysis (DEA) with multiple DEA executions considering all combinations of inputs and outputs to calculate efficiency scores. The methodology reduces possible biases in the selection of resources and products of the model, ability to support decision-making in favor of improvements in the sector′s efficiency based on national regulatory framework. The analyses show that the companies analyzed can increase their operating results and attendance coverage by more than 60%, given the current levels of infrastructure, human and financial resources in the sector. Based on the simulation of potential efficiency gains in Brazilian basic sanitation companies, the estimates show that the coverage of the population with access to sanitary sewage would go from the current 59.9% to 76.5%. The evidence found provides indications to subsidize sanitation management in the country at the micro-analytical level, enabling a better competitive position in the sector for the integrated management of basic sanitation and its universalization in Brazil.
Analysis of Pedestrian Street-Crossing Decision-Making Based on Vehicle Deceleration-Safety Gap
Authors: Zhang Hongjia , Guo Yingshi , Chen Yunxing , Sun Qinyu , Wang Chang
Published: December 2020

Numerous traffic crashes occur every year on zebra crossings in China. Pedestrians are vulnerable road users who are usually injured severely or fatally during human-vehicle collisions. The development of an effective pedestrian street-crossing decision-making model is essential to improving pedestrian street-crossing safety. For this purpose, this paper carried out a naturalistic field experiment to collect a large number of vehicle and pedestrian motion data. Through interviewed with many pedestrians, it is found that they pay more attention to whether the driver can safely brake the vehicle before reaching the zebra crossing. Therefore, this work established a novel decision-making model based on the vehicle deceleration-safety gap (VD-SGM). The deceleration threshold of VD-SGM was determined based on signal detection theory (SDT). To verify the performance of VD-SGM proposed in this work, the model was compared with the Raff model. The results show that the VD-SGM performs better and the false alarm rate is lower. The VD-SGM proposed in this work is of great significance to improve pedestrians’ safety. Meanwhile, the model can also increase the efficiency of autonomous vehicles.
Maximal Time Spent at VO2max from Sprint to the Marathon
Authors: Molinari Claire A. , Edwards Johnathan , Billat Véronique
Published: December 2020

Until recently, it was thought that maximal oxygen uptake (VO2max) was elicited only in middle-distance events and not the sprint or marathon distances. We tested the hypothesis that VO2max can be elicited in both the sprint and marathon distances and that the fraction of time spent at VO2max is not significantly different between distances. Methods: Seventy-eight well-trained males (mean [SD] age: 32 [13]; weight: 73 [9] kg; height: 1.80 [0.8] m) performed the University of Montreal Track Test using a portable respiratory gas sampling system to measure a baseline VO2max. Each participant ran one or two different distances (100 m, 200 m, 800 m, 1500 m, 3000 m, 10 km or marathon) in which they are specialists. Results: VO2max was elicited and sustained in all distances tested. The time limit (Tlim) at VO2max on a relative scale of the total time (Tlim at VO2max%Ttot) during the sprint, middle-distance, and 1500 m was not significantly different (p > 0.05). The relevant time spent at VO2max was only a factor for performance in the 3000 m group, where the Tlim at VO2max%Ttot was the highest (51.4 [18.3], r = 0.86, p = 0.003). Conclusions: By focusing on the solicitation of VO2max, we demonstrated that the maintenance of VO2max is possible in the sprint, middle, and marathon distances.
TLR7/8 in the Pathogenesis of Parkinson’s Disease
Authors: Campolo Michela , Filippone Alessia , Biondo Carmelo , Mancuso Giuseppe , Casili Giovanna , Lanza Marika , Cuzzocrea Salvatore , Esposito Emanuela , Paterniti Irene
Published: December 2020

Neuroinflammation and autoimmune mechanisms have a key part in the pathogenesis of Parkinson’s disease (PD). Therefore, we evaluated the role of Toll-like receptors (TLRs) as a link between inflammation and autoimmunity in PD. An in vivo model of PD was performed by administration of 1-metil 4-fenil 1,2,3,6-tetraidro-piridina (MPTP) at the dose of 20 mg/kg every 2 h for a total administration of 80/kg, both in single Knock Out (KO) mice for TLR7, TLR 8, and TLR9 and in double KO mice for TLR 7/8-/-. All animals were compared with WT animals used as a control group. All animals were sacrificed after 7 days form the first administration of MPTP. The genetic absence of TLR 7 and 8 modified the PD pathway, increasing the immunoreactivity for TH and DAT compared to PD groups and decreasing microglia and astrocytes activation. Moreover, the deletion of TLR7 and TLR8 significantly reduced T-cell infiltration in the substantia nigra and lymph nodes, suggesting a reduction of T-cell activation. Therefore, our result highlights a possibility that an immunotherapy approach, by using a dual antagonist of TLR 7 and 8, could be considered as a possible target to develop new therapies for Parkinson diseases.
Association of Soluble Suppression of Tumorigenesis-2 (ST2) with Endothelial Function in Patients with Ischemic Heart Failure
Authors: Dimitropoulos Stathis , Mystakidi Vasiliki Chara , Oikonomou Evangelos , Siasos Gerasimos , Tsigkou Vasiliki , Athanasiou Dimitris , Gouliopoulos Nikolaos , Bletsa Evanthia , Kalampogias Aimilios , Charalambous Georgios , Tsioufis Costas , Vavuranakis Manolis , Tousoulis Dimitris
Published: December 2020

Soluble suppression of tumorigenesis-2 (sST2) has been introduced as a marker associated with heart failure (HF) pathophysiology and status. Endothelial dysfunction is a component underlying HF pathophysiology. Therefore, we examined the association of arterial wall properties with sST2 levels in patients with HF of ischemic etiology. We enrolled 143 patients with stable HF of ischemic etiology and reduced left ventricular ejection fraction (LVEF) and 77 control subjects. Flow-mediated dilation (FMD) was used to evaluate endothelial function and pulse wave velocity (PWV) to assess arterial stiffness. Although there was no significant difference in baseline demographic characteristics, levels of sST2 were increased in HF compared to the control (15.8 (11.0, 21.8) ng/mL vs. 12.5 (10.4, 16.3) ng/mL; p < 0.001). In the HF group, there was a positive correlation of sST2 levels with age (rho = 0.22; p = 0.007) while there was no association of LVEF with sST2 (rho = −0.119; p = 0.17) nor with PWV (rho = 0.1; p = 0.23). Interestingly, sST2 was increased in NYHA III [20.0 (12.3, 25.7) ng/mL] compared to patients with NYHA II (15.0 (10.4, 18.2) ng/mL; p = 0.003) and inversely associated with FMD (rho = −0.44; p < 0.001) even after adjustment for possible confounders. In patients with chronic HF of ischemic etiology, sST2 levels are increased and are associated with functional capacity. There is an inverse association between FMD and sST2 levels, highlighting the interplay between the dysfunctional endothelium and HF pathophysiologic mechanisms.
The Ubiquitin E3 Ligase MaLUL2 Is Involved in High Temperature-Induced Green Ripening in Banana Fruit
Authors: Wei Wei , Chen Jian-ye , Zeng Ze-xiang , Kuang Jian-fei , Lu Wang-jin , Shan Wei
Published: December 2020

Harvested banana fruit ripened under warm temperatures above 24 °C remain green peel, leading to severe economic loss. E3 ubiquitin-ligases, as the major components in the ubiquitination pathway, have been implicated to play important roles in temperature-stress responses. However, the molecular mechanism underlying high temperature-triggered stay-green ripening bananas in association with E3 ubiquitin-ligases, remains largely unknown. In this study, a RING-type E3 ubiquitin ligase termed MaLUL2, was isolated and characterized from banana fruit. The MaLUL2 gene contains 1095 nucleotides and encodes a protein with 365 amino acids. The MaLUL2 protein contains a domain associated with RING2 (DAR2) and a RING domain, which are the typical characteristics of RING-type E3 ligases. MaLUL2 expression was up-regulated during high temperature-induced green ripening. Subcellular localization showed that MaLUL2 localized in the nucleus, cytoplasm, and plasma membrane. MaLUL2 displayed E3 ubiquitin ligase activity in vitro. More importantly, transient overexpression of MaLUL2 in banana fruit peel increased the level of ubiquitination in vivo and led to a stay-green phenotype, accompanying with decreased expression of chlorophyll catabolic genes. Collectively, these findings suggest that MaLUL2 might act as a negative regulator of chlorophyll degradation and provide novel insights into the regulatory mechanism of high temperature-induced green ripening bananas.
BIRC6 Is Associated with Vulnerability of Carotid Atherosclerotic Plaque
Authors: Alloza Iraide , Salegi Andrea , Mena Jorge , Navarro Raquel Tulloch , Martin César , Aspichueta Patricia , Salazar Lucía Martínez , Carpio Jon Uriarte , Cagigal Patricia De-la-Hera , Vega Reyes , Triviño Juan Carlos , Freijo Maria del Mar , Vandenbroeck Koen
Published: December 2020

Carotid atherosclerotic plaque rupture can lead to cerebrovascular accident (CVA). By comparing RNA-Seq data from vascular smooth muscle cells (VSMC) extracted from carotid atheroma surgically excised from a group of asymptomatic and symptomatic subjects, we identified more than 700 genomic variants associated with symptomatology (p < 0.05). From these, twelve single nucleotide polymorphisms (SNPs) were selected for further validation. Comparing genotypes of a hospital-based cohort of asymptomatic with symptomatic patients, an exonic SNP in the BIRC6 (BRUCE/Apollon) gene, rs35286811, emerged as significantly associated with CVA symptomatology (p = 0.002; OR = 2.24). Moreover, BIRC6 mRNA levels were significantly higher in symptomatic than asymptomatic subjects upon measurement by qPCR in excised carotid atherosclerotic tissue (p < 0.0001), and significantly higher in carriers of the rs35286811 risk allele (p < 0.0001). rs35286811 is a proxy of a GWAS SNP reported to be associated with red cell distribution width (RDW); RDW was increased in symptomatic patients (p < 0.03), but was not influenced by the rs35286811 genotype in our cohort. BIRC6 is a negative regulator of both apoptosis and autophagy. This work introduces BIRC6 as a novel genetic risk factor for stroke, and identifies autophagy as a genetically regulated mechanism of carotid plaque vulnerability.
In Vivo Evaluation of a Miniaturized Fluorescence Molecular Tomography (FMT) Endoscope for Breast Cancer Detection Using Targeted Nanoprobes
Authors: Yang Hao , Qian Weipin , Yang Lily , Xie Huikai , Jiang Huabei
Published: December 2020

In this study, in vivo animal experiments with 12 nude mice bearing breast-cancer-patient-tissue-derived xenograft (PDX) tumors were performed aiming to verify the imaging capability of a novel miniaturized fluorescence molecular tomography (FMT) endoscope, in combination with targeted nanoparticle–near-infrared (NIR) dye conjugates. Tumor-bearing mice were divided into two groups by systematic injection with urokinase plasminogen activator receptor-targeted (n = 7) and nontargeted (n = 5) imaging nanoprobes as a contrast agent, respectively. Each mouse was imaged at 6, 24, and 48 h following the injection of nanoprobes using the FMT endoscope. The results show that systemic delivery of targeted nanoprobes produced a 4-fold enhancement in fluorescence signals from tumors, compared with tumors that received nontargeted nanoprobes. This study indicates that our miniaturized FMT endoscope, coupled with the targeted nanoparticle–NIR dye conjugates as a contrast agent, has high sensitivity and specificity, and thus great potential to be used for image-guided detection and removal of a primary tumor and local metastatic tumors during surgery.
The Role of Nitric Oxide in Cancer: Master Regulator or NOt?
Authors: Khan Faizan H. , Dervan Eoin , Bhattacharyya Dibyangana D. , McAuliffe Jake D. , Miranda Katrina M. , Glynn Sharon A.
Published: December 2020

Nitric oxide (NO) is a key player in both the development and suppression of tumourigenesis depending on the source and concentration of NO. In this review, we discuss the mechanisms by which NO induces DNA damage, influences the DNA damage repair response, and subsequently modulates cell cycle arrest. In some circumstances, NO induces cell cycle arrest and apoptosis protecting against tumourigenesis. NO in other scenarios can cause a delay in cell cycle progression, allowing for aberrant DNA repair that promotes the accumulation of mutations and tumour heterogeneity. Within the tumour microenvironment, low to moderate levels of NO derived from tumour and endothelial cells can activate angiogenesis and epithelial-to-mesenchymal transition, promoting an aggressive phenotype. In contrast, high levels of NO derived from inducible nitric oxide synthase (iNOS) expressing M1 and Th1 polarised macrophages and lymphocytes may exert an anti-tumour effect protecting against cancer. It is important to note that the existing evidence on immunomodulation is mainly based on murine iNOS studies which produce higher fluxes of NO than human iNOS. Finally, we discuss different strategies to target NO related pathways therapeutically. Collectively, we present a picture of NO as a master regulator of cancer development and progression.
Liposomes Targeting P21 Activated Kinase-1 (PAK-1) and Selective for Secretory Phospholipase A2 (sPLA2) Decrease Cell Viability and Induce Apoptosis in Metastatic Triple-Negative Breast Cancer Cells
Authors: Najahi-Missaoui Wided , Quach Nhat D. , Somanath Payaningal R. , Cummings Brian S.
Published: December 2020

P21 activated kinases (or group I PAKs) are serine/threonine kinases whose expression is altered in prostate and breast cancers. PAK-1 activity is inhibited by the small molecule “Inhibitor targeting PAK-1 activation-3” (IPA-3), which has selectivity for PAK-1 but is metabolically unstable. Secretory Group IIA phospholipase A2 (sPLA2) expression correlates to increased metastasis and decreased survival in many cancers. We previously designed novel liposomal formulations targeting both PAK-1 and sPLA2, called Secretory Phospholipase Responsive liposomes or SPRL-IPA-3, and demonstrated their ability to alter prostate cancer growth. The efficacy of SPRL against other types of cancers is not well understood. We addressed this limitation by determining the ability of SPRL to induce cell death in a diverse panel of cells representing different stages of breast cancer, including the invasive but non-metastatic MCF-7 cells, and metastatic triple-negative breast cancer (TNBC) cells such as MDA-MB-231, MDA-MB-468, and MDA-MB-435. We investigated the role of sPLA2 in the disposition of these liposomes by comparing the efficacy of SPRL-IPA-3 to IPA-3 encapsulated in sterically stabilized liposomes (SSL-IPA-3), a formulation shown to be less sensitive to sPLA2. Both SSL-IPA-3 and SPRL-IPA-3 induced time- and dose-dependent decreases in MTT staining in all cell lines tested, but SPRL-IPA-3-induced effects in metastatic TNBC cell lines were superior over SSL-IPA-3. The reduction in MTT staining induced by SPRL-IPA-3 correlated to the expression of Group IIA sPLA2. sPLA2 expression also correlated to increased induction of apoptosis in TNBC cell lines by SPRL-IPA-3. These data suggest that SPRL-IPA-3 is selective for metastatic TNBC cells and that the efficacy of SPRL-IPA-3 is mediated, in part, by the expression of Group IIA sPLA2.
Overview of Epstein–Barr-Virus-Associated Gastric Cancer Correlated with Prognostic Classification and Development of Therapeutic Options
Authors: De Re Valli , Brisotto Giulia , Repetto Ombretta , De Zorzi Mariangela , Caggiari Laura , Zanussi Stefania , Alessandrini Lara , Canzonieri Vincenzo , Miolo Gianmaria , Puglisi Fabio , Belluco Claudio , Steffan Agostino , Cannizzaro Renato
Published: December 2020

Gastric cancer (GC) is a deadly disease with poor prognosis that is characterized by heterogeneity. New classifications based on histologic features, genotypes, and molecular phenotypes, for example, the Cancer Genome Atlas subtypes and those by the Asian Cancer Research Group, help understand the carcinogenic differences in GC and have led to the identification of an Epstein–Barr virus (EBV)-related GC subtype (EBVaGC), providing new indications for tailored treatment and prognostic factors. This article provides a review of the features of EBVaGC and an update on the latest insights from EBV-related research with a particular focus on the strict interaction between EBV infection and the gastric tumor environment, including the host immune response. This information may help increase our knowledge of EBVaGC pathogenesis and the mechanisms that sustain the immune response of patients since this mechanism has been demonstrated to offer a survival advantage in a proportion of patients with GC.