bluuebunny/crossref_metadata_2025 huggingface dataset:
Angle-stable interlocking nailing in a canine critical-sized femoral defect model for bone regeneration studies: In pursuit of the principle of the 3R’s
Authors: Saunders W. B. , Dejardin L. M. , Soltys-Niemann E. V. , Kaulfus C. N. , Eichelberger B. M. , Dobson L. K. , Weeks B. R. , Kerwin S. C. , Gregory C. A.
Published: September 2022

Introduction: Critical-sized long bone defects represent a major therapeutic challenge and current treatment strategies are not without complication. Tissue engineering holds much promise for these debilitating injuries; however, these strategies often fail to successfully translate from rodent studies to the clinical setting. The dog represents a strong model for translational orthopedic studies, however such studies should be optimized in pursuit of the Principle of the 3R’s of animal research (replace, reduce, refine). The objective of this study was to refine a canine critical-sized femoral defect model using an angle-stable interlocking nail (AS-ILN) and reduce total animal numbers by performing imaging, biomechanics, and histology on the same cohort of dogs.

Methods: Six skeletally mature hounds underwent a 4 cm mid-diaphyseal femoral ostectomy followed by stabilization with an AS-ILN. Dogs were assigned to autograft (n = 3) or negative control (n = 3) treatment groups. At 6, 12, and 18 weeks, healing was quantified by ordinal radiographic scoring and quantified CT. After euthanasia, femurs from the autograft group were mechanically evaluated using an established torsional loading protocol. Femurs were subsequently assessed histologically.

Results: Surgery was performed without complication and the AS-ILN provided appropriate fixation for the duration of the study. Dogs assigned to the autograft group achieved radiographic union by 12 weeks, whereas the negative control group experienced non-union. At 18 weeks, median bone and soft tissue callus volume were 9,001 mm3 (range: 4,939–10,061) for the autograft group and 3,469 mm3 (range: 3,085–3,854) for the negative control group. Median torsional stiffness for the operated, autograft treatment group was 0.19 Nm/° (range: 0.19–1.67) and torque at failure was 12.0 Nm (range: 1.7–14.0). Histologically, callus formation and associated endochondral ossification were identified in the autograft treatment group, whereas fibrovascular tissue occupied the critical-sized defect in negative controls.

Conclusion: In a canine critical-sized defect model, the AS-ILN and described outcome measures allowed refinement and reduction consistent with the Principle of the 3R’s of ethical animal research. This model is well-suited for future canine translational bone tissue engineering studies.
A new mouse model of ankle instability induced by multiple mechanical sprains with controlled inversion angle and speed
Authors: Ching Shih-Hong , Chiu Yen-Chun , Liao Yu-Ching , Yang Shang-Hsun , Tsai Yi-Ju
Published: September 2022

Ankle sprain occurs by a sudden and extreme inversion and plantarflexion at the ankle joint to cause ligamentous injuries. A portion of ankle sprain patients experience recurrent ankle sprains and develop chronic ankle instability (CAI). The present CAI animal models are single events with severe ligamentous injury using surgical transection of ligaments or manually overextending the ankle.

Purpose: To simulate the mechanical and recurrent sprain injuries in CAI patients, we established a new ankle instability model with multiple ankle injuries using a self-designed machine to sprain the ankle with a controlled inversion angle and speed.

Methods: Male C57BL/6J mice were used and respectively subjected to a sham operation, calcaneofibular ligament (CFL) transection, and mechanical ankle sprains. Three mechanical sprains were performed on the 13th and 185th day after the initial mechanical ankle sprain.

Results: The first mechanical sprain and CFL transection induced ankle injury as indicated by an average of a 62% decrease in ankle pressure pain threshold and a 114% increase in the ankle thickness compared with the contralateral untreated ankle. The second and third mechanical sprains induced recurrent ankle injuries. The foot slips during beam tests were increased after mechanical ankle sprains but not after CFL transection, indicating the induction of motor balance deficits. Multiple mechanical ankle sprains induced significant gait changes in longer duration of stance (an average of 194% increase), swing (134%), and step cycle (147%) compared with CFL transection or sham operation, and slower walking speed (78% reduction) and shorter step distance (91%) after the third sprain.

Conclusion: These results elucidate that multiple mechanical sprains, which induce recurrent ankle injuries, balance deficits, and gait changes, are a good model for investigating the mechanisms of CAI induced by recurrent sprain injuries.
An ex-vivo model for the biomechanical assessment of cement discoplasty
Authors: Ghandour Salim , Pazarlis Konstantinos , Lewin Susanne , Isaksson Per , Försth Peter , Persson Cecilia
Published: September 2022

Percutaneous Cement Discoplasty (PCD) is a surgical technique developed to relieve pain in patients with advanced degenerative disc disease characterized by a vacuum phenomenon. It has been hypothesized that injecting bone cement into the disc improves the overall stability of the spinal segment. However, there is limited knowledge on the biomechanics of the spine postoperatively and a lack of models to assess the effect of PCD ex-vivo. This study aimed to develop a biomechanical model to study PCD in a repeatable and clinically relevant manner. Eleven ovine functional spinal units were dissected and tested under compression in three conditions: healthy, injured and treated. Injury was induced by a papain buffer and the treatment was conducted using PMMA cement. Each sample was scanned with micro-computed tomography (CT) and segmented for the three conditions. Similar cement volumes (in %) were injected in the ovine samples compared to volumes measured on clinical PCD CT images. Anterior and posterior disc heights decreased on average by 22.5% and 23.9% after injury. After treatment, the anterior and posterior disc height was restored on average to 98.5% and 83.6%, respectively, of their original healthy height. Compression testing showed a similar stiffness behavior between samples in the same group. A decrease of 51.5% in segment stiffness was found after injury, as expected. The following PCD treatment was found to result in a restoration of stiffness—showing only a difference of 5% in comparison to the uninjured state. The developed ex-vivo model gave an adequate representation of the clinical vacuum phenomena in terms of volume, and a repeatable mechanical response between samples. Discoplasty treatment was found to give a restoration in stiffness after injury. The data presented confirm the effectiveness of the PCD procedure in terms of restoration of axial stiffness in the spinal segment. The model can be used in the future to test more complex loading scenarios, novel materials, and different surgical techniques.
Fluorescence lifetime imaging of NAD(P)H upon oxidative stress in Kluyveromyces marxianus
Authors: Ai Yi , Luo Ruoyu , Yang Deqiang , Ma Jiong , Yu Yao , Lu Hong
Published: September 2022

K. marxianus is a promising cell factory for producing heterologous proteins. Oxidative stresses were raised during overexpression of heterologous proteins, leading to the shift of the redox state. How to measure the redox state of live K. marxianus cells without perturbing their growth remains a big challenge. Here, a fluorescence lifetime imaging (FLIM)-based method was developed in live K. marxianus cells. During the early exponential growth, K. marxianus cells exhibited an increased mean fluorescence lifetime (τ-mean) of NAD(P)H compared with Saccharomyces cerevisiae cells, which was consistent with the preference for respiration in K. marxianus cells and that for fermentation in S. cerevisiae cells. Upon oxidative stresses induced by high temperature or H2O2, K. marxianus cells exhibited an increased τ-mean in company with decreased intracellular NAD(P)H/NAD(P)+, suggesting a correlation between an increased τ-mean and a more oxidized redox state. The relationship between τ-mean and the expression level of a heterologous protein was investigated. There was no difference between the τ-means of K. marxianus strains which were not producing a heterologous protein. The τ-mean of a strain yielding a high level of a heterologous protein was higher than that of a low-yielding strain. The results suggested the potential application of FLIM in the non-invasive screen of high-yielding cells.
Thermal analysis of horizontal earth-air heat exchangers in a subtropical climate: An experimental study
Authors: Lattieff Farkad A. , Atiya Mohammed A. , Lateef Rudainah Ali , Dulaimi Anmar , Jweeg Muhsin J. , Abed Azher M. , Mahdi Jasim M. , Talebizadehsardari Pouyan
Published: September 2022

The earth-air heat exchanger (EHX) has a promising potential to passively save the energy consumption of traditional air conditioning systems while maintaining a high degree of indoor comfort. The use of EHX systems for air conditioning in commercial and industrial settings offers several environmental benefits and is capable of operating in both standalone and hybrid modes. This study tests the performance and effectiveness of an EHX design in a sandy soil area in Baghdad, Iraq. The area has a climate of the subtropical semi-humid type. Ambient air temperatures and soil temperatures were recorded throughout the months of 2021. During the months of January and June, the temperatures of the inlet and outflow air at varying air velocities were monitored concurrently in 10-min increments at each location. Further numerical and thermodynamical analyses of the measurements were conducted to reveal the influencing performance parameters. The highest temperature rises of air between the input and exit sections were determined as 12.3°C (January) and 17.2°C (June). It is found that the maximum values of effectiveness are 0.80 and 0.81, while coefficients of performance are 1.6 and 1.8 for January and June, respectively. It is also found that the EHX shows good functionality and effectiveness, with potential energy savings for equipment for cooling and heating under different weather conditions.
Health risk assessment of heavy metals in soils and food crops from a coexist area of heavily industrialized and intensively cropping in the Chengdu Plain, Sichuan, China
Authors: Liu Qing , Li Xiaohui , He Lei
Published: September 2022

Environmental pollution caused by rapid industrial activities are becoming increasingly drastic, particularly its impact on soil and plant health. The present study was conducted to investigate the heavy metal (loid) (As, Cd, Cu, Hg, Pb, and Zn) concentrations in soils and food biomass crops and estimate the potential health risks of metals to humans via consumption of contaminated food biomass crops from Shifang, a periurban agricultural areas in the Chengdu Plain, Sichuan, China. Results revealed that the soils have been experiencing a substantial accumulation of heavy metals, especially for Cd, with a mean of 0.84 mg kg−1, about six times higher than the background values, of which 98% exceeded the pollution warning threshold of the China Soil Environmental Quality Standards. A total of 78% of all the grain part failed the national food standard for Cd. No significantly positive relationships between metal levels in food biomass crops and in the corresponding soils, indicated metals enrichment in soils were not entirely reflected to crops contaminant burdens. Estimated daily intake (EDI) of all the metals except for Pb, exceeded the oral reference dose (RfD) or the minimal risk levels recommended by USEPA and ATSDR. Target hazard quotients (THQs) of all the metals except for Cd was less than one indicated that potential health risk to the local inhabitant originated mainly from Cd exposure via cereals consumption. Mitigation strategies to curtail Cd-contaminated soils and crops Cd burdens need careful tailoring to meet the needs of health and safety in this region.
Evaluation of environmental Mucorales contamination in and around the residence of COVID-19-associated mucormycosis patients
Authors: Ghosh Anup K. , Singh Ravinder , Reddy Snigdha , Singh Shreya , Rudramurthy Shivaprakash M. , Kaur Harsimran , Choudhary Hansraj , Chakrabarti Arunaloke
Published: September 2022

Introduction

Recently, India witnessed an unprecedented surge of coronavirus disease 2019 (COVID-19)-associated mucormycosis (CAM) cases. In addition to patient management issues, environmental Mucorales contamination possibly contributed to the outbreak. A recent study evaluated environment contamination by Mucorales in the hospital setting. However, a considerable number of CAM patients were never admitted to a hospital before the development of the disease. The present study, therefore, planned to evaluate Mucorales contamination of patients’ residences.

Methods

The residential environment of 25 patients with CAM living in north India was surveyed. Air samples were collected from indoor and immediate outdoor vicinity of the patients’ residence and cultured on Dichloran Rose–Bengal Chloramphenicol (DRBC) agar with benomyl for selective isolation of Mucorales. Surface swab samples were also collected from the air coolers fitted in those residences and cultured on DRBC agar. The isolates were identified by phenotypic and genotypic methods. Amplified fragment length polymorphism (AFLP) was employed to evaluate the genetic relatedness of the environmental and patients’ clinical isolates.

Results

The median spore count (mean ± SD, cfu/m3) of Mucorales in the air of patients’ bedrooms was significantly higher than in the air in other rooms in those residences (3.55 versus 1.5, p = 0.003) or the air collected directly from the front of the air cooler (p < 0.0001). The Mucorales spore count in the environment did not correlate with either ventilation of the room or hygiene level of the patients’ residences. Rhizopus arrhizus was isolated from the environment of all patients’ residences (n = 25); other Mucorales species isolated were Cunninghamella bertholletiae (n = 14), Rhizopus microsporus (n = 6), Rhizopus delemar (n = 6), Syncephalastrum racemosum (n = 1), Lichtheimia corymbifera (n = 1), and Mucor racemosus (n = 1). Genetic relatedness was observed between 11 environmental isolates from the patients’ bedrooms and respective clinical isolates from patients.

Discussion

The study supported the view that the patients might have acquired Mucorales from the home environment during the post-COVID-19 convalescence period. Universal masking at home during patients’ convalescence period and environmental decontamination could minimize exposure in those susceptible patients.
Guidelines for evaluating the conservation value of African lion (Panthera leo) translocations
Authors: Becker Matthew S. , Almeida Joao , Begg Colleen , Bertola Laura , Breitenmoser Christine , Breitenmoser Urs , Coals Peter , Funston Paul , Gaylard Angela , Groom Rosemary , Henschel Philipp , Ikanda Dennis , Jorge Agostinho , Kruger Johan , Lindsey Peter , Maimbo Howard , Mandisodza-Chikerema Roseline , Maude Glynn , Mbizah Moreangels , Miller Susan M. , Mudongo Edwin , Mwape Henry , Mweetwa Thandiwe , Naude Vincent , Nyirenda Vincent R. , Parker Andrew , Parker Daniel , Reid Craig , Robson Ashley , Sayer Ed , Selier SA Jeanetta , Sichande Mwamba , Simukonda Chuma , Uiseb Kenneth , Williams Vivienne L. , Zimba Dennis , Hunter Luke
Published: September 2022

As the top predator in African ecosystems, lions have lost more than 90% of their historical range, and few countries possess strong evidence for stable populations. Translocations (broadly defined here as the capture and movement of lions for various management purposes) have become an increasingly popular action for this species, but the wide array of lion translocation rationales and subsequent conservation challenges stemming from poorly conceived or unsuitable translocations warrants additional standardized evaluation and guidance. At their best, translocations fill a key role in comprehensive strategies aimed at addressing the threats facing lions and fostering the recovery of wild populations in their historic range. At their worst, translocations can distract from addressing the major threats to wild populations and habitats, divert scarce funding from more valuable conservation actions, exacerbate conflict with humans in recipient sites, disrupt local lion demography, and undermine the genetic integrity of wild lion populations in both source and recipient sites. In the interest of developing best practice guidelines for deciding when and how to conduct lion translocations, we discuss factors to consider when determining whether a translocation is of conservation value, introduce a value assessment for translocations, and provide a decision matrix to assist practitioners in improving the positive and reducing the negative outcomes of lion translocation.
Inflammatory bowel disease and cardiovascular disease: A two-sample Mendelian randomization analysis
Authors: Wu Kaiwen , Li Aoshuang , Liu Lei , Shu Tao , Xia Demeng , Sun Xiaobin
Published: September 2022

Background

Although epidemiological studies have shown a positive relationship between inflammatory bowel disease (IBD) and risk of cardiovascular disease (CVD) outcomes, a solid causal relationship has not been established. Thus, a two-sample Mendelian randomization (MR) study was conducted to explore the potential causal effect between IBD and CVD outcomes.

Methods

We performed a two-sample MR analysis to analyze the causal effect of the IBD on CVD outcome by using summary-level genome-wide association studies of European descent. The inverse-variance weighted (IVW) method was used as the main MR analysis, with complementary analyses of MR Egger, maximum likelihood, weighted median, penalized weighted media, simple mode, weighted mode, and MR-PRESSO methods. Multiple sensitivity analyses were used to evaluate the robustness of our results.

Results

All P-values were greater than 0.05 in the IVW method, showing no evidence of a causal association between circulating IBD and CVD. Similar results were observed by using other MR methods. No evidence of heterogeneity, pleiotropy, or outlier single-nucleotide polymorphisms was detected. Sensitivity analyses demonstrated the robustness of the results.

Conclusion

The findings of this study provided no evidence to support that IBD has a large effect on risk of CVD outcomes, which is in contrast to many previous observational reports. Further studies are needed to determine the potential mechanism of association identified in observational studies.
A newly designed disk-lobe occluder with isogenous barbs for left atrial appendage closure: Initial multicenter experience
Authors: Bai Yuan , Tang Xuechao , Xu Xudong , Zhao Xianxian , Xu Yawei , Chen Wei , Zhu Xianyang , Wang Qiguang , Han Zhihua , Wang Changqian , He Lu , Zhang Yushun , Pan Xin , Wang Cheng , Chen Lianglong , Cen Xuejiang , Qu Baiming , Zhu Ni , Zhang Sha , Huang Xinmiao , Qin Yongwen
Published: September 2022

Background

Although the implant success rate of left atrial appendage closure (LAAC) has increased and complications have decreased over time, there are still anatomically and technically complicated cases where novel LAA occluders may simplify the procedure and thus might potentially improve the clinical outcome.

Objectives

This study aimed to assess the safety and efficacy of the newly designed device with isogenous barbs in LAAC.

Methods

Eight centers in China participated in this prospective study from July 2016 to April 2018. Peri- and post-procedural safety and efficacy were evaluated through scheduled follow-ups and transesophageal echocardiography (TEE).

Results

A total of 175 patients with a mean age of 68.4 ± 9.2 years old, a mean CHA2DS2-VASc score of 4.7 ± 1.8, and a mean HAS-BLED score of 3.2 ± 1.3, were included. The device was successfully implanted in 173 patients (98.9%). The device size ranged from 18 to 34 mm. Clinically relevant pericardial effusion (PEF) in the perioperative period, occurred in 3 patients (1.7%). TEE follow-up was available in 167 (96.5%) patients at 12-month. During follow-up, 9 patients suffered serious adverse event: 4 death (2.3%), 1 ischemic stroke (0.6%), and 2 gastro-intestinal bleeding (1.2%) and 2 device-related thrombus (DRT) (1.2%). Estimated annual thromboembolism rate reduced by 90% and estimated annual major bleeding rate reduced by 81% after LAAC with the newly designed device.

Conclusion

The newly designed device with isogenous barbs for LAAC could be performed effectively with a low incidence of adverse events and a high incidence of anatomic closure.
Sleep duration, daytime napping, and risk of incident stroke: Nuances by metabolic syndrome from the China health and retirement longitudinal study
Authors: Fang Yuanyuan , He Yuqin , Huang Yanzhu , Ran Lusen , Song Wenhui , Hao Jiahuan , Yao Di , Li Rong , Pan Dengji , Qin Tingting , Wang Minghuan
Published: September 2022

Background and purpose

The relationship between sleep duration and stroke are inconclusive in China, especially in those individuals with metabolic syndrome. We aimed to investigate the association between sleep duration and incident stroke in participants with metabolic syndrome or its specific components in China.

Materials and methods

Data were taken from the 2011 and 2015 waves of China Health and Retirement Longitudinal Study (CHARLS). Habitual sleep duration (≤6, 6∼8 [reference], >8 h), daytime napping (0, 1∼60 [reference], and >60 min) were determined by self-reported questionnaires. Metabolic syndrome was defined by blood assessment and biomarkers combined with self-reported doctors’ diagnosis. Incident stroke was determined by reported stroke from 2011 to 2015 wave. Cross-sectional and longitudinal associations between sleep and (incident) stroke at baseline and 4-year follow-up period were tested among the population with metabolic syndrome and its components.

Results

A U-shaped relationship was observed between sleep duration and stroke in cross-sectional analysis. Sleep ≤ 6 h/night had a greater risk of incident stroke (hazard ratio [HR] 1.65; 95% confidence interval [CI] 1.04–2.61) compared with sleep 6∼8 h/night. And the HR of stroke was 1.62 (95%CI, 1.03–2.53) for sleep < 7 h/day compared to 7∼9 h/day. These associations were more evident in the female and individuals aged 45–65 years. Furthermore, the effect of short sleep duration on incident stroke was different in each component of metabolic syndrome, which was more pronounced in participants with elevated blood pressure. And a significant joint effect of sleeping ≤ 6 h/night and no napping on risk of stroke was observed (HR 1.82, 95%CI 1.06–3.12).

Conclusion

Short sleep duration was an independent risk factor for incident stroke, especially among females, individuals aged 45–65 years, or those with some components of metabolic syndrome, such as hypertension. Napping could buffer the risk of short sleep duration on incident stroke.
Stratigraphy and eruption history of maars in the Clear Lake Volcanic Field, California
Authors: Ball Jessica L.
Published: September 2022

The Clear Lake Volcanic Field (CLVF) is the northernmost and youngest field in a chain of volcanic fields in the California Coast Range mountains. Effusive and explosive volcanic activity in the field has spanned at least 2.1 million years, with the youngest eruptions comprising a series of maar craters at the edges of, and within, Clear Lake itself. This work documents the first direct ages for many of these maar deposits and builds the stratigraphic basis for interpreting eruptive processes and dynamics of the young eruptions which produced them. Detailed stratigraphy has distinguished maar eruption products from pyroclastic deposits (monolithologic falls and flows, previously mapped together with maars as a single unit) and established a set of six eruption facies from maar deposit lithology, grain size parameters, and depositional structures. Radiocarbon dates from carbon films found on clasts at three outcrops have constrained several of these maar eruptions to ∼8,500–13,500 years BP, coinciding with eruptive periods previously estimated based on lake core tephrachronology. Part of this period also coincides with indigenous inhabitation (<12,000 years BP), which suggests that oral histories of Pomo and other local tribes may contain descriptions of volcanic phenomena experienced by local residents of the CLVF. Collaboration between volcanologists and indigenous historians may add a valuable human dimension to the youngest eruptions of the Clear Lake Volcanic Field, and help inform future volcanic hazard assessment.
An improved method of GB-SAR phase unwrapping for landslide monitoring
Authors: Xiang Xia , Chen Chen , Wang Hui , Xing Cheng , Chen Jiankang , Zhu Hong
Published: September 2022

The ground-based Synthetic Aperture Radar (GB-SAR) technique can be applied to the safety monitoring and early warning of geo-hazards, especially for monitoring displacement of various types of landslide masses. One of the key techniques of processing GB-SAR monitoring data is phase unwrapping, which can be dramatically affected by atmospheric humidity, atmospheric pressure, sampling interval, etc. In high mountains and valleys where environmental change is drastic, GB-SAR monitoring data is vulnerable to incoherence both spatially and temporally. Therefore, an improved phase unwrapping method of GB-SAR data for landslide monitoring is proposed in this paper, which can realize three-dimensional phase unwrapping in time and space. The method adopts the idea of sparse data processing and realizes phase unwrapping of monitoring data in two steps. Firstly, taking full advantage of the high temporal resolution of GB-SAR monitoring data, the one-dimensional phase unwrapping method in the spatial domain is applied to the time domain, and the interference phases of PS points are unwrapped in the time domain. Then, the PS network is constructed based on the time-phase unwrapping results, the double-difference phases of the network baselines are obtained and the spatial consistency adjustment is applied to them, and the spatial phase unwrapping can be realized by the indirect least square adjustment method. This method successfully solves the problem of path dependence, island isolation and large computation of the common method of space-borne SAR phase unwrapping, which can process GB-SAR data with high accuracy and efficiency.
Analysis of changes in the hydrological regime in Lalin River basin and its impact on the ecological environment
Authors: Wang Xuege , Ma Wenchao , Lv Jun , Li Hongyan , Liu Hongchao , Mu Guangyi , Bian Dejun
Published: September 2022

Lalin River is a first-level tributary of the right bank of Songhua River. High-quality corn production bases irrigated by the Lalin River are famous throughout the country. However, in the last few decades, the hydrological regimes changed severely due to expansion of the irrigation area, leading to more water used for irrigation. To investigate the variation of the hydrological situation, we analyzed the daily runoff data at the Caijiagou Hydrological Station from 1954 to 2015 by using the Indicators of Hydrologic Alteration (IHA) and the range of variability approach (RVA). The results showed a severe decreasing trend of the average monthly flow during the water level with regular and high periods, with a negative slope of −2.924 in August and −2.518 in September. The increasing trend of low pulse and fall rate of flow and the decreasing trend of the rising rate of flow demonstrate that water resources are drying up. Regarding the flow rate, the annual maximum value decreased significantly in slopes of −7.9, −7.6, −6.09, −5.28, and −2.98 on the 1-day, 3-day, 7-day, 30-day, and 90-day, respectively. The flow reversals’ average value is 65 to 50 times/year from 1954 to 2015. From RVA analysis, the hydrological alteration of the basin decreased 50% after 1978. To investigate the influencing factors for the hydrological alteration, we analyzed the variation in land cover and land use between 1978 and 2015. The results showed that the area of paddy fields in 2015 was 1.5 times that of 1978, and the water consumption in paddy fields accounts for 80.8% of the total water resources. Consequently, the wetland area of the basin in 2015 was only 53% of 1978, and the wetland area of the two wetland nature reserves at the mouth of the Lalin River in 2015 was about 53% of 1978. Furthermore, the meteorological condition in the LRB shows a warming and drying tendency. The air temperature has shown an intensively increased tendency, and precipitation decreased in the last 50 years. This indicates that the hydrological situation in the Lalin River Basin has changed considerably under the dual influence of human activities and global warming.
Determinants of teachers’ attitudes toward inclusion of pupils with attention deficit hyperactivity disorder: The role of teacher education
Authors: Flavian Heidi , Uziely Eran
Published: September 2022

Given that teachers’ attitudes are an essential constituent of most academic and social processes taking place in their classrooms, the authors of the current study examined teachers’ attitudes and their origins specifically toward the inclusion of pupils with ADHD, taking into account demographic variables, teachers’ professionalism, and either a personal diagnosis of ADHD or prior acquaintance with it through relatives. The data, gathered from 475 participants’ responses to questionnaires, revealed that professional teacher training was only a secondary factor influencing attitudes regarding the inclusion of children with ADHD. A more prominent factor was teachers’ own diagnosis of ADHD or prior experience with children/relatives with ADHD. Study results are followed by practical suggestions for improving teacher education based on these findings.
Molecular and cellular mechanisms in diabetic heart failure: Potential therapeutic targets
Authors: Mengstie Misganaw Asmamaw , Abebe Endeshaw Chekol , Teklemariam Awgichew Behaile , Mulu Anemut Tilahun , Teshome Assefa Agegnehu , Zewde Edgeit Abebe , Muche Zelalem Tilahun , Azezew Muluken Teshome
Published: September 2022

Diabetes Mellitus (DM) is a worldwide health issue that can lead to a variety of complications. DM is a serious metabolic disorder that causes long-term microvascular and macro-vascular complications, as well as the failure of various organ systems. Diabetes-related cardiovascular diseases (CVD) including heart failure cause significant morbidity and mortality worldwide. Concurrent hypertensive heart disease and/or coronary artery disease have been thought to be the causes of diabetic heart failure in DM patients. However, heart failure is extremely common in DM patients even in the absence of other risk factors such as coronary artery disease and hypertension. The occurrence of diabetes-induced heart failure has recently received a lot of attention. Understanding how diabetes increases the risk of heart failure and how it mediates major cellular and molecular alteration will aid in the development of therapeutics to prevent these changes. Hence, this review aimed to summarize the current knowledge and most recent findings in cellular and molecular mechanisms of diabetes-induced heart failure.
Comparative study of modern heuristic algorithms for global maximum power point tracking in photovoltaic systems under partial shading conditions
Authors: Wang Kangshi , Ma Jieming , Man Ka Lok , Huang Kaizhu , Huang Xiaowei
Published: September 2022

Under partial shading conditions (PSCs), photovoltaic (PV) generation systems exhibit multiple local and a single global maximum power point. Consequently, global maximum power point tracking (GMPPT) is required to improve the performance of PV systems in such scenarios. This paper comparatively studies and evaluates the tracking performance of modern heuristic-optimization-based GMPPT techniques. Monte Carlo method is used to statistically analyze different methods. Simulation and experimental results indicate that many of the algorithms have difficulties in balancing the explorative and exploitative searching behaviors. Therefore, we propose a variable vortex search (VVS), which is capable of improving the performance of GMPPT by using a variable step size and deterministic starting points. This paper will aid researchers and practical engineers to gain a thorough understanding on how to use modern heuristic algorithms for maximum power out of PV systems. Furthermore, it offers a comprehensive guidance on how to perform efficiently GMPPT in the PV systems under PSCs.
Analysis of fractional MHD convective flow with CTNs’ nanoparticles and radiative heat flux in human blood
Authors: Aleem Maryam , Inc Mustafa , Sarwar Shahzad , Asjad Muhammad Imran , Alsubaie A. S. A.
Published: September 2022

The aim of the article is two-fold. We first analyze and investigate free convective, unsteady, MHD blood flow with single- and multiwalled carbon nanotubes (S&MWCNTs) as nanoparticles. The blood flow has been taken across an upright vertical plate, oscillating in its own plane, and engrafted in a porous medium with slip, radiation, and porosity effects. Nanofluids consist of human blood as the base fluid and SWCNTs and MWCNTs as nanoparticles. The second aim is to discuss the three different definitions of fractional derivatives, namely, Caputo (C), Caputo–Fabrizio (CF), and Atangana–Baleanu (ABC), to obtain the solutions of such proposed models by the Adomian decomposition method. The impact of fractional and physical parameters on the concentration, velocity, and temperature of human blood in the presence of the slip effect is studied and projected diagrammatically. The article ends by providing numerical results such as the reliableness, efficiency, and significant features that are simple in computation with eminent accuracy of the process for non-Newtonian Casson nanofluid fractional order models. It is observed that the velocity of the fluid decreases with SWCNTs’ and MWCNTs’ volume fraction, and an increase in the CNTs’ volume fraction increases blood temperature, which ultimately enhances heat transfer rates. The results acquired are in excellent correspondence with the reported results.
Co-application of organic amendments and inorganic P increase maize growth and soil carbon, phosphorus availability in calcareous soil
Authors: Khan Khuram Shehzad , Ali Muhammad Moaaz , Naveed Muhammad , Rehmani Muhammad Ishaq Asif , Shafique Muhammad Waleed , Ali Hayssam M. , Abdelsalam Nader R. , Ghareeb Rehab Y. , Feng Gu
Published: September 2022

Phosphorus (P) constraint can be alleviated by increasing C inputs, which can help to improve crop production and P fertilizer use efficiency. However, the effects of different manures on soil microbial biomass P (MBP) and P fractions as well as C fractions in calcareous soils remain poorly understood. Soil MBP pool involves the P mineralization and immobilization processes, potentially changing P fractions and P availability. Therefore, the effects of different manures on soil microbial biomass (MBP, MBC) pool, P, and C fractions and crop P utilization were evaluated in greenhouse experiments with maize plantation. Treatments included no manure (control), poultry manure (PM), cow manure (CM), goat manure (GM), mixed manure (MM), and three inorganic P (Pi) rates; P0: 0 mg kg-1, P50: 50 mg kg-1, and P100: P100 mg kg-1 (P2O5). For plant growth comparison, crop physiological growth indices, shoot P contents and total P uptake were increased by PM and P100 as compared to other treatments. The PM with P100 significantly increased the plant growth by inducing P uptake of ∼18% compared with control. The results exhibited that Pi (P100) combined with manure (PM) significantly (p < 0.05) increased the soil physicochemical properties, that is, 683.76 mg kg-1 total P, 21.5 mg kg-1 Olsen P, 4.26 g kg-1 SOC, 2.41 g kg-1 POC, as well as microbial biomass C and P increased by 152.84 mg kg-1 and 36.83 mg kg-1, respectively. Consequently, we concluded that PM with Pi (P100) application builds up soil microbial biomass, which is more beneficial for promoting P utilization for maize.
How does D&O insurance affect corporate environmental investment?
Authors: Liu Jiamin , Jiang Yalin , Gan Shengdao
Published: September 2022

Directors’ and officers’ liability insurance (D&O insurance), an important tool for diversifying and transferring risks of managers, plays a crucial role in corporate investment decisions, including corporate environmental investment decisions. However, the relationship between D&O insurance and corporate environmental investment remains unknown. Using a sample of Chinese listed firms, this study examines whether and how D&O insurance affects corporate environmental investment from 2008 to 2019. We find that D&O insurance is negatively associated with corporate environmental investment. This result is consistent with the results of a series of robustness tests. Further analyses show that D&O insurance impedes corporate environmental investment by driving executives to seek private benefits, especially monetary benefits. Moreover, the negative effect of D&O insurance on corporate environmental investment is more pronounced in low-polluting and highly competitive industries. However, this negative relationship is mitigated by political connections. The findings contribute to the literature by providing empirical evidence of the involvement of D&O insurance in influencing corporate environmental investment decisions.
Climate risk and corporate cash holdings: Mechanism and path analysis
Authors: Yu Siming , Wang Lei , Zhang Shuocheng
Published: September 2022

Based on climate change data of local cities and cash holding data of Chinese listed companies from 2011 to 2019, this article studies the impact of climate change on the level of corporate cash holdings and analyzes the path of climate change on the corporate cash holding level. The results show that under the influence of climate change, enterprises hold a high level of cash holdings to cope with risks and change variables to carry out robustness tests; meanwhile, the results remain consistent. By analyzing the source channels of enterprise cash, it is found that climate change increases the cash received from selling goods, providing services, and tax refund, but decreases the cash received from borrowing. The mechanism of action shows that climate change has increased the operating risk of enterprises and thus the level of cash holdings of enterprises, but climate change has not increased the financial risk of enterprises. The main reason is that under the influence of climate change, enterprises have reduced the debt level and thus reduced the financial risk. Heterogeneity analysis shows that the impact of climate change on corporate cash holdings is more obvious in state-owned enterprises, enterprises with high degree of financing constraints, and ecologically fragile regions. This study provides a new explanation for the influencing factors and approaches of corporate cash management strategies, which helps to better understand the cash holding strategies of listed companies in China. It also indicates that climate change is likely to be a macro incentive to aggravate the risks of real economy.
Length of stay and departure strategies of Semipalmated Sandpipers (Calidris pusilla) during post-breeding migration in the upper Bay of Fundy, Canada
Authors: Neima Sarah G. , Linhart Rebeca C. , Hamilton Diana J. , Gratto-Trevor Cheri L. , Paquet Julie
Published: September 2022

The Bay of Fundy, Canada is a critical staging area for Semipalmated Sandpipers (Calidris pusilla) during post-breeding migration. Recent range-wide population declines and changes in diet and migratory timing in the Bay of Fundy prompted a re-examination of staging ecology, including length of stay (last estimated in 1981), which is used in calculating migratory population estimates. We used radio-telemetry and the Motus Wildlife Tracking System to estimate individual length of stay and departure conditions for 159 Semipalmated Sandpipers in 2013 and 2014. Using tracking data we compared two estimation methods, minimum length of stay and mark-recapture modelling. Using minimum length of stay, the mean length of stay was approximately 21 days, an increase from the previous estimate of 15 days. Mark-recapture models suggested a much longer staging period that is inconsistent with other data. Sandpipers captured early in the staging period stayed longer on average than those captured later. Departures from the staging area were correlated with north-westerly winds, moderate to high wind speeds and low but rising atmospheric pressures. We suggest that Semipalmated Sandpipers in the Bay of Fundy are not operating on a time-selected migration schedule and instead wait for favourable weather conditions to depart, which occur more often later in the migratory period. Population trends in the Bay of Fundy should be re-evaluated in light of the increased length of stay.
Individual variation, personality, and the ability of animals to cope with climate change
Authors: Cockrem John F.
Published: September 2022

The Sixth Assessment of the Intergovernmental Panel on Climate Change describes negative effects of climate change on animals occurring on a larger scale than previously appreciated. Animal species are increasingly experiencing more frequent and extreme weather in comparison with conditions in which the species evolved. Individual variation in behavioural and physiological responses of animals to stimuli from the environment is ubiquitous across all species. Populations with relatively high levels of individual variation are more likely to be able to survive in a range of environmental conditions and cope with climate change than populations with low levels of variation. Behavioural and physiological responses are linked in animals, and personality can be defined as consistent individual behavioural and physiological responses of animals to changes in their immediate environment. Glucocorticoids (cortisol and corticosterone) are hormones that, in addition to metabolic roles, are released when the neuroendocrine stress system is activated in response to stimuli from the environment perceived to be threatening. The size of a glucocorticoid response of an animal is an indication of the animal’s personality. Animals with reactive personalities have relatively high glucocorticoid responses, are relatively slow and thorough to explore new situations, and are more flexible and able to cope with changing or unpredictable conditions than animals with proactive personalities. Animals with reactive personalities are likely to be better able to cope with environmental changes due to climate change than animals with proactive personalities. A reaction norm shows the relationship between phenotype and environmental conditions, with the slope of a reaction norm for an individual animal a measure of phenotypic plasticity. If reaction norm slopes are not parallel, there is individual variation in plasticity. Populations with relatively high individual variation in plasticity of reaction norms will have more animals that can adjust to a new situation than populations with little variation in plasticity, so are more likely to persist as environments change due to climate change. Future studies of individual variation in plasticity of responses to changing environments will help understanding of how populations of animals may be able to cope with climate change.
No functional contribution of the gustatory receptor, Gr64b, co-expressed in olfactory sensory neurons of Drosophila melanogaster
Authors: Mahadevan Venkatesh Pal , Lavista-Llanos Sofía , Knaden Markus , Hansson Bill S.
Published: September 2022

Chemosensation is essential for the survival of insects. Activities like searching for food, mating, and oviposition in the fruit fly, Drosophila melanogaster are to a great extent governed by chemical cues detected via olfaction and gustation. This chemical information is conveyed to higher brain centers via populations of diverse olfactory sensory neurons (OSNs) and gustatory sensory neurons (GSNs) expressing olfactory receptors (ORs) and gustatory receptors (GRs), respectively. ORs are exclusively expressed in the antenna and in the maxillary palps, while GRs are widely expressed in the labellum, tarsi, genitalia etc. Interestingly, 14 GRs were previously reported to be expressed in the antenna of D. melanogaster. However, the spatial expression pattern for all GRs and their functional role are still unclear. Recent data challenge the dogma that single OSNs express a single OR. In the present study, we studied the expression of 12 previously reported GRs among sensory structures on the fly antenna using the Gal4-UAS binary expression system. We observed antennal expression of nine out of the 12 reported. Out of these nine, consistent expression was only apparent for Gr64b, and we reconfirmed its presence in OSNs innervating three glomeruli in the antennal lobe. These glomeruli are known to be innervated by ab5A, ab5B and ab8A OSNs, respectively. Next, we generated double labeling crosses with Gr64b and observed co-expression of Gr64b with Or47a, which is expressed in the ab5B neuron. To elucidate the functional role of Gr64b co-expressed with Or47a, we challenged Or47a-expressing OSNs in wild type and Gr64b–/– mutant flies with odor stimulation using the single sensillum recording technique in two satiation states (fed and starved). Notably, we did not observe any significant odor sensitivity or specificity changes in Gr64b mutants as compared to wild type flies. Taken together, our results reveal co-expression of GRs with ORs in olfactory sensory neurons, while the functional contribution of the GR in this context remains obscure.
Genetic polymorphisms of PKLR gene and their associations with milk production traits in Chinese Holstein cows
Authors: Du Aixia , Zhao Fengru , Liu Yanan , Xu Lingna , Chen Kewei , Sun Dongxiao , Han Bo
Published: September 2022

Our previous work had confirmed that pyruvate kinase L/R (PKLR) gene was expressed differently in different lactation periods of dairy cattle, and participated in lipid metabolism through insulin, PI3K-Akt, MAPK, AMPK, mTOR, and PPAR signaling pathways, suggesting that PKLR is a candidate gene to affect milk production traits in dairy cattle. Here, we verified whether this gene has significant genetic association with milk yield and composition traits in a Chinese Holstein cow population. In total, we identified 21 single nucleotide polymorphisms (SNPs) by resequencing the entire coding region and partial flanking region of PKLR gene, in which, two SNPs were located in 5′ promoter region, two in 5′ untranslated region (UTR), three in introns, five in exons, six in 3′ UTR and three in 3′ flanking region. The single marker association analysis displayed that all SNPs were significantly associated with milk yield, fat and protein yields or protein percentage (p ≤ 0.0497). The haplotype block containing all the SNPs, predicted by Haploview, had a significant association with fat yield and protein percentage (p ≤ 0.0145). Further, four SNPs in 5′ regulatory region and eight SNPs in UTR and exon regions were predicted to change the transcription factor binding sites (TFBSs) and mRNA secondary structure, respectively, thus affecting the expression of PKLR, leading to changes in milk production phenotypes, suggesting that these SNPs might be the potential functional mutations for milk production traits in dairy cattle. In conclusion, we demonstrated that PKLR had significant genetic effects on milk production traits, and the SNPs with significant genetic effects could be used as candidate genetic markers for genomic selection (GS) in dairy cattle.