bluuebunny/crossref_metadata_2025 huggingface dataset:
Dependency on Suppliers as a Peril in the Acquisition of Innovations? The Role of Buyer Attractiveness in Mitigating Potential Negative Dependency Effects in Buyer–Supplier Relations
Authors: Schiele Holger , Vos Frederik G.S.
Published: June 2015

New product development occurs nowadays mostly in joint buyer–supplier projects, which require closer ties between the partners in order to mobilize their resources. One issue arising from this collaborative model is that the buyer tends to become more dependent on the supplier. Multiple cases of supplier obstructionism have been reported. To mitigate this dilemma, this paper analyzes the relevance of customer attractiveness as an enabler of collaboration. Testing this hypothesis on a sample of 218 buyer–supplier relationships, we show that dependency as such is not the issue in the presence of close ties. Buyers who are a preferred customer of their suppliers can accept the risk of becoming dependent on them. The managerial implications of this finding is that firms should apply a reverse marketing approach and thus attempt to become the preferred customers of their important suppliers. From a conceptual perspective, our findings indicate the need to consider dependency not as an isolated variable, but in conjunction with attractiveness.
Magic and the British Middle Classes, 1750–1900
Authors: Waters Thomas
Published: June 2015

This essay explores the attitudes of the British middle classes towards witchcraft, ghosts, and other so-called superstitions from the mid-eighteenth to the late nineteenth centuries. Conventional historiographical wisdom maintains that belief in magic among middle-class Britons declined gradually between the early modern and modern eras. Grounded in the study of newspapers, antiquarianism, public lectures, and literary fiction, this essay proposes a more precise chronology for the decline and subsequent resurgence of magic. It argues that it was only from the 1820s that the middle classes, the media that served them, the police, and certain politicians put popular superstitions under significant duress, and examines the agendas and anxieties underlying this temporary cultural shift away from magic. The later Victorian period saw the emergence of greater tolerance towards witchcraft and ghost beliefs, allowing them to be reinterpreted as picturesque folklore or fitting subjects for the enquiries of psychical science.
On the transport of heavy particles through a downward displacement-ventilated space
Authors: Mingotti Nicola , Woods Andrew W.
Published: June 2015

We investigate the transport of relatively heavy, small particles through a downward displacement-ventilated space. A flux of particles is supplied to the space from a localised source at a high level and forms a turbulent particle-laden plume which descends through the space. A constant flow of ambient fluid which does not contain particles is supplied to the space at a high level, while an equal amount of fluid is vented from the space at a low level. As a result of the entrainment of ambient fluid into the particle plume, a return flow is produced in the ambient fluid surrounding the plume in the lower part of the space. At steady state, particles are suspended by this return flow. An interface is formed which separates the ambient fluid in the lower part of the space, which contains particles, from the particle-free ambient fluid in the upper part of the space. New laboratory experiments show that the concentration of particles in the ambient fluid below the interface is larger than the average concentration of particles in the plume fluid at the level of the interface. Hence, as the plume fluid crosses the interface and descends through the particle-laden fluid underneath, it becomes relatively buoyant and forms a momentum-driven fountain. If the fountain fluid impinges on the floor, it then spreads radially over the surface until lifting off. We develop a quantitative model which can predict the height of the interface, the concentration of particles in the lower layer, and the partitioning of the particle flux between the fraction which sediments over the floor and that which is ventilated out of the space. We generalise the model to show that when particles and negatively buoyant fluid are supplied at the top of the space, a three-layer stratification develops in the space at steady state: the upper layer contains relatively low-density ambient fluid in which no particles are suspended; the central layer contains a mixture of ambient and plume fluid in which no particles are suspended; and the lower layer contains a suspension of particles in the same mixture of ambient and plume fluid. We quantify the heights of the two interfaces which separate the three layers in the space and the concentration of particles in suspension in the ambient fluid in the lower layer. We then discuss the relevance of the results for the control of airborne infections in buildings. Our experiments show that the three-layer stratification is subject to intermittent large-scale instabilities when the concentration of particles in the plume at the source is sufficiently small, or the rate of ventilation of the space is sufficiently large: we describe the transient concentration of particles in the space during one of these instabilities.
Bifurcation structure of two-dimensional viscous zonal flows on a rotating sphere
Authors: Sasaki Eiichi , Takehiro Shin-ichi , Yamada Michio
Published: June 2015

We study the bifurcation structure of zonal flows on a rotating sphere. The setting of our problem is similar to the Kolmogorov problem on a flat torus, where the vorticity forcing is given by a single eigenfunction of the Laplacian. First we prove the global stability of two-jet zonal flow for arbitrary Reynolds number and the rotation rate of the sphere. Then we study the bifurcation structure of steady solutions arising from three-jet zonal flow. In the non-rotating case, we find that two steady travelling-wave solutions bifurcate from a three-jet zonal flow via Hopf bifurcation. As the Reynolds number increases, steady-travelling solutions arise via pitchfork bifurcation from the steady-travelling solutions. On the other hand, in the rotating case, we find saddle-node bifurcations and closed-loop branches. We carry out time integration to study the properties of unsteady solutions at high Reynolds numbers. In the non-rotating case, the unsteady solution is chaotic and it wanders around the steady-travelling solutions bifurcating from three-jet zonal flow. We show that a linear combination of the steady and steady-travelling solutions gives a good approximation of the zonal-mean zonal flow of the unsteady solution, suggesting that the chaotic solution at high Reynolds numbers exists mostly within a relatively low-dimensional space spanned by the steady and steady-travelling solutions, which become unstable at low Reynolds numbers.
Alternative Education Engaging Indigenous Young People: Flexi Schooling in Queensland
Authors: Shay Marnee , Heck Deborah
Published: June 2015

This article will discuss some of the findings from a qualitative research project that explored the connections between alternative education and Indigenous learners. This study investigated how flexi school leaders reported they were supporting Indigenous young people to remain engaged in education. The results of the survey provide demographic data focusing on Indigenous participation in this sample of flexi schools. The results revealed that a high number of Indigenous young people are participating in flexi schools within this sample. Furthermore, a high number of Indigenous staff members are working in multiple roles within these schools. The implications of these findings are twofold. First, the current Indigenous education policy environment is focused heavily on ‘Closing the Gap’, emphasising the urgent need for significant improvement of educational outcomes for Indigenous young people. The findings from this study propose that flexi schools are playing a significant role in supporting Indigenous young people to remain engaged in education, yet there remains a limited focus on this within the literature and education policy. Second, the high participation rates of Indigenous young people and staff suggest an urgent need to explore this context through research. Further research will assist in understanding the culture of the flexi school context. Research should also explore why a high number of Indigenous young people and staff members participate in this educational context and how this could influence the approach to engagement of Indigenous young people in conventional school settings.
Determinants of Independent Living Optimism among Adults with Multiple Sclerosis
Authors: Li Jian , Wilson Hannah , Fitzgerald Shawn M. , Bishop Malachy , Rumrill Phillip D.
Published: June 2015

This study investigated the relationship between cognitive, psychological and physical symptoms of multiple sclerosis (MS) and independent living optimism among a sample of 5,082 adults with MS (females = 77.8%; Whites = 96.1%, age range 20 to 91 years). Data on physical, cognitive and psychological function were collected utilising standardised clinical measures including the Multiple Sclerosis Impact Scale-29, Perceived Deficits Questionnaire-5-Item version, and the Independent Living Optimism scale. Multiple regression analysis indicated that specific physical, psychological and cognitive factors contributed to the overall prediction model. The combined variables explained approximately 33% of the variance in independent living optimism. This study enables a specific, item-level understanding of the relationship between MS-related symptoms and independent living optimism, and provides important information for the development of targeted rehabilitation interventions.
Light Curve Solutions of Ten Eccentric Kepler Binaries, Three of them with Tidally Induced Humps
Authors: Kjurkchieva D. , Vasileva D.
Published: June 2015

We carried out light curve solutions of ten detached eclipsing eccentric binaries observed by Kepler. The formal errors of the derived parameters from the light curve solutions are below 1%. Our results give indications that the components of the eccentric binaries (especially those with mass ratios below 0.5) do not follow precisely the empirical relations between the stellar parameters derived from the study of circular-orbit binaries. We found the following peculiarities of the targets: (a) the components of KIC 9474969 have almost the same temperatures while their radii and masses differ by a factor around 2.5; (b) KIC 6949550 reveals semi-regular light variations with an amplitude of 0.004 and a period around 7 d which are modulated by long-term variations; (c) KIC 6220470, KIC 11071207, and KIC 9474969 exhibit tidally induced ‘hump’ around the periastron. These are the targets with the biggest relative radii of our sample. We derived the dependence of the hump amplitude on the relative stellar radii, eccentricity, and mass ratio of eccentric binary consisting of MS stars.
Inversion is Possible in Groups with no Periodic Automorphisms
Authors: Bridson Martin R. , Short Hamish
Published: June 2015

There exist infinite finitely presented torsion-free groups G such that Aut(G) and Out(G) are torsion free but G has an automorphism sending some non-trivial element to its inverse.
Evidenced-based management of haemoptysis by otolaryngologists
Authors: Bannister M , Ah-See K W
Published: June 2015

Background:

Haemoptysis is an uncommon presenting symptom to the ENT clinic and ward, but has potentially sinister aetiology. This article aims to provide a systematic and evidence-based method of managing patients with haemoptysis.

Methods:

The data in this article are based on a literature search performed using PubMed in August 2013. The keywords used included ‘haemoptysis’ in combination with ‘otolaryngology’, ‘ENT’, ‘head & neck', ‘diagnosis’, ‘management’, ‘investigations’ and ‘treatment’.

Results:

The majority of published literature on the subject is level IV evidence. However, this can guide ENT specialists in assessing, investigating and managing presentations of haemoptysis.

Conclusion:

Understanding the different causes of haemoptysis is important for the otolaryngologist. The main concern is the detection of a malignant lesion in the upper aerodigestive tract or tracheobronchial tree. A thorough history and systematic examination can aid diagnosis.
Dexmedetomidine improves the quality of the operative field for functional endoscopic sinus surgery: systematic review
Authors: Snidvongs K , Tingthanathikul W , Aeumjaturapat S , Chusakul S
Published: June 2015

Background:

Intra-operative bleeding diminishes visualisation during functional endoscopic sinus surgery and can cause unfavourable outcomes. Dexmedetomidine is a potent alpha-2 agonist, with sympatholytic effects. This systematic review aimed to assess whether dexmedetomidine decreases intra-operative bleeding and improves operative field quality.

Methods:

All randomised, controlled trials that assessed the ability of dexmedetomidine to provide good operative fields for functional endoscopic sinus surgery were identified from Medline and Embase. The outcomes of interest were: operative field quality, intra-operative bleeding, operative time and adverse events.

Results:

Five studies (254 patients) met the inclusion criteria. When compared to saline, dexmedetomidine improved the quality of the operative field. The operative time was similar between groups. When compared to other drugs, dexmedetomidine was as effective as esmolol and remifentanil. There were no adverse incidents.

Conclusion:

Dexmedetomidine is beneficial in providing good visibility during functional endoscopic sinus surgery. Controlled hypotensive anaesthesia with this medicine decreases intra-operative bleeding and enhances surgical field quality.
Positron clouds within thunderstorms
Authors: Dwyer Joseph R. , Smith David M. , Hazelton Bryna J. , Grefenstette Brian W. , Kelley Nicole A. , Lowell Alexander W. , Schaal Meagan M. , Rassoul Hamid K.
Published: June 2015

We report the observation of two isolated clouds of positrons inside an active thunderstorm. These observations were made by the Airborne Detector for Energetic Lightning Emissions (ADELE), an array of six gamma-ray detectors, which flew on a Gulfstream V jet aircraft through the top of an active thunderstorm in August 2009. ADELE recorded two 511 keV gamma-ray count rate enhancements, 35 s apart, each lasting approximately 0.2 s. The enhancements, which were approximately a factor of 12 above background, were both accompanied by electrical activity as measured by a flat-plate antenna on the underside of the aircraft. The energy spectra were consistent with a source mostly composed of positron annihilation gamma rays, with a prominent 511 keV line clearly visible in the data. Model fits to the data suggest that the aircraft was briefly immersed in clouds of positrons, more than a kilometre across. It is not clear how the positron clouds were created within the thunderstorm, but it is possible they were caused by the presence of the aircraft in the electrified environment.
The allometry of oysters: spatial and temporal variation in the length–biomass relationships forCrassostrea virginica
Authors: Powell Eric N. , Mann Roger , Ashton-Alcox Kathryn A. , Kim Yungkul , Bushek David
Published: June 2015

We examine the relationship of biomassBand lengthLin the eastern oysterCrassostrea virginicaby focusing on the scaling exponentbin the allometric equationB=aLbusing four datasets: Delaware Bay, Chesapeake Bay, Galveston Bay and a regionally extensive compilation from the NOAA Mussel Watch Program. The average value of the scaling exponent in Delaware Bay and Chesapeake Bay is about 2. For Galveston Bay, the value is distinctly higher, near 2.6. Over all Mussel Watch sites, the value is again near 2. Within Delaware Bay, the salinity gradient exerts an important effect. Shells are longer for their meat weight at lower salinities. The range of scaling exponents revealed by Mussel Watch data is exceedingly large (b< 1 to >3). Scaling exponents below 2.5 are unusual in bivalves. Among bivalves, only other oyster taxa have comparably low scaling exponents averaging near 2. We propose that oyster biomass routinely scales nearer the square of the length rather than the cube and that this is a constraint imposed by the exigency of carbonate production for reef maintenance and accretion in the face of high rates of taphonomic degradation. The adaptation as a reef builder requires the formation of carbonate that rapidly breaks down, thus requiring that carbonate produced be maximized. A biomass-to-length scaling exponent of 2 provides a mechanism to maximize shell production relative to biomass, while at the same time providing maximum surface area for the all-important settling of oyster spat to maintain the population.
Vocabulary size, translation equivalents, and efficiency in word recognition in very young bilinguals
Authors: LEGACY JACQUELINE , ZESIGER PASCAL , FRIEND MARGARET , POULIN-DUBOIS DIANE
Published: June 2015

The present study examined early vocabulary development in fifty-nine French monolingual and fifty French–English bilingual infants (1;4–1;6). Vocabulary comprehension was assessed using both parental report (MacArthur-Bates Communicative Development Inventory; CDI) and the Computerized Comprehension Task (CCT). When assessing receptive vocabulary development using parental report, the bilinguals knew more words in their L1 versus their L2. However, young bilinguals were as accurate in L1 as they were in L2 on the CCT, and exhibited no difference in speed of word comprehension across languages. The proportion of translation equivalents in comprehension varied widely within this sample of young bilinguals and was linked to both measures of vocabulary size but not to speed of word retrieval or exposure to L2. Interestingly, the monolinguals outperformed the bilinguals with respect to accuracy but not reaction time in their L1 and L2. These results highlight the importance of using multiple measures to assess early vocabulary development.
Climate change and the Ethiopian economy: a CGE analysis
Authors: Gebreegziabher Zenebe , Stage Jesper , Mekonnen Alemu , Alemu Atlaw
Published: June 2015

The paper analyzes the economic impacts of climate change-induced fluctuations on the performance of Ethiopia's agriculture, using a countrywide computable general equilibrium (CGE) model. We model the impacts on agriculture using a Ricardian model, where current agricultural production is modelled as a function of temperature and precipitation, among other things, and where future agriculture is assumed to follow the same climate function. The effect of overall climate change is projected to be relatively benign until approximately 2030, but will become considerably worse thereafter. Our simulation results indicate that, over a 50-year period, the projected reduction in agricultural productivity may lead to reductions in average income of some 20 per cent compared with the outcome that would have prevailed in the absence of climate change. This indicates that adaptation policies – both government planned and those that ease autonomous adaptation by farmers – will be crucial for Ethiopia's future development.
Metagenomic resolution of microbial functions in deep-sea hydrothermal plumes across the Eastern Lau Spreading Center
Authors: Anantharaman Karthik , Breier John A , Dick Gregory J
Published: June 2015

Microbial processes within deep-sea hydrothermal plumes affect ocean biogeochemistry on global scales. In rising hydrothermal plumes, a combination of microbial metabolism and particle formation processes initiate the transformation of reduced chemicals like hydrogen sulfide, hydrogen, methane, iron, manganese and ammonia that are abundant in hydrothermal vent fluids. Despite the biogeochemical importance of this rising portion of plumes, it is understudied in comparison to neutrally buoyant plumes. Here we use metagenomics and bioenergetic modeling to describe the abundance and genetic potential of microorganisms in relation to available electron donors in five different hydrothermal plumes and three associated background deep-sea waters from the Eastern Lau Spreading Center located in the Western Pacific Ocean. Three hundred and thirty one distinct genomic ‘bins’ were identified, comprising an estimated 951 genomes of archaea, bacteria, eukarya and viruses. A significant proportion of these genomes is from novel microorganisms and thus reveals insights into the energy metabolism of heretofore unknown microbial groups. Community-wide analyses of genes encoding enzymes that oxidize inorganic energy sources showed that sulfur oxidation was the most abundant and diverse chemolithotrophic microbial metabolism in the community. Genes for sulfur oxidation were commonly present in genomic bins that also contained genes for oxidation of hydrogen and methane, suggesting metabolic versatility in these microbial groups. The relative diversity and abundance of genes encoding hydrogen oxidation was moderate, whereas that of genes for methane and ammonia oxidation was low in comparison to sulfur oxidation. Bioenergetic-thermodynamic modeling supports the metagenomic analyses, showing that oxidation of elemental sulfur with oxygen is the most dominant catabolic reaction in the hydrothermal plumes. We conclude that the energy metabolism of microbial communities inhabiting rising hydrothermal plumes is dictated by the underlying plume chemistry, with a dominant role for sulfur-based chemolithoautotrophy.
Directed assembly of a bacterial quorum
Authors: Servinsky Matthew D , Terrell Jessica L , Tsao Chen-Yu , Wu Hsuan-Chen , Quan David N , Zargar Amin , Allen Patrick C , Byrd Christopher M , Sund Christian J , Bentley William E
Published: June 2015

Many reports have elucidated the mechanisms and consequences of bacterial quorum sensing (QS), a molecular communication system by which bacterial cells enumerate their cell density and organize collective behavior. In few cases, however, the numbers of bacteria exhibiting this collective behavior have been reported, either as a number concentration or a fraction of the whole. Not all cells in the population, for example, take on the collective phenotype. Thus, the specific attribution of the postulated benefit can remain obscure. This is partly due to our inability to independently assemble a defined quorum, for natural and most artificial systems the quorum itself is a consequence of the biological context (niche and signaling mechanisms). Here, we describe the intentional assembly of quantized quorums. These are made possible by independently engineering the autoinducer signal transduction cascade of Escherichia coli (E. coli) and the sensitivity of detector cells so that upon encountering a particular autoinducer level, a discretized sub-population of cells emerges with the desired phenotype. In our case, the emergent cells all express an equivalent amount of marker protein, DsRed, as an indicator of a specific QS-mediated activity. The process is robust, as detector cells are engineered to target both large and small quorums. The process takes about 6 h, irrespective of quorum level. We demonstrate sensitive detection of autoinducer-2 (AI-2) as an application stemming from quantized quorums. We then demonstrate sub-population partitioning in that AI-2-secreting cells can ‘call’ groups neighboring cells that ‘travel’ and establish a QS-mediated phenotype upon reaching the new locale.
Fano resonance boosted cascaded optical field enhancement in a plasmonic nanoparticle-in-cavity nanoantenna array and its SERS application
Authors: Zhu Zhendong , Bai Benfeng , You Oubo , Li Qunqing , Fan Shoushan
Published: June 2015

Cascaded optical field enhancement (CFE) can be realized in some specially designed multiscale plasmonic nanostructures, in which the generation of extremely strong fields at nanoscale volume is crucial for many applications, for example, surface-enhanced Raman spectroscopy (SERS). In this paper, we propose a strategy for realizing a high-quality plasmonic nanoparticle-in-cavity (PIC) nanoantenna array, in which strong coupling between a nanoparticle (NP) dark mode with a high-order nanocavity bright mode can produce strong Fano resonance at the target wavelength. The Fano resonance can effectively boost the CFE in a PIC. A cost-effective and reliable nanofabrication method is developed using room temperature nanoimprinting lithography to manufacture high-quality PIC arrays. This technique guarantees the generation of only one gold NP at the bottom of each nanocavity, which is crucial for the generation of the expected CFE. To demonstrate the performance and application of the PIC array, the PIC array is employed as an active SERS substrate for detecting 4-aminothiophenol molecules. An experimental SERS enhancement factor of 2 × 107 is obtained, which verifies the field enhancement and the potential of this device.
Phase stability and dynamics of entangled polymer–nanoparticle composites
Authors: Mangal Rahul , Srivastava Samanvaya , Archer Lynden A.
Published: June 2015

Nanoparticle–polymer composites, or polymer–nanoparticle composites (PNCs), exhibit unusual mechanical and dynamical features when the particle size approaches the random coil dimensions of the host polymer. Here, we harness favourable enthalpic interactions between particle-tethered and free, host polymer chains to create model PNCs, in which spherical nanoparticles are uniformly dispersed in high molecular weight entangled polymers. Investigation of the mechanical properties of these model PNCs reveals that the nanoparticles have profound effects on the host polymer motions on all timescales. On short timescales, nanoparticles slow-down local dynamics of the host polymer segments and lower the glass transition temperature. On intermediate timescales, where polymer chain motion is typically constrained by entanglements with surrounding molecules, nanoparticles provide additional constraints, which lead to an early onset of entangled polymer dynamics. Finally, on long timescales, nanoparticles produce an apparent speeding up of relaxation of their polymer host.
Plasmon coupling in vertical split-ring resonator metamolecules
Authors: Wu Pin Chieh , Hsu Wei-Lun , Chen Wei Ting , Huang Yao-Wei , Liao Chun Yen , Liu Ai Qun , Zheludev Nikolay I. , Sun Greg , Tsai Din Ping
Published: June 2015

The past decade has seen a number of interesting designs proposed and implemented togenerate artificial magnetism at optical frequencies using plasmonic metamaterials,but owing to the planar configurations of typically fabricated metamolecules thatmake up the metamaterials, the magnetic response is mainly driven by the electricfield of the incident electromagnetic wave. We recently fabricated verticalsplit-ring resonators (VSRRs) which behave as magnetic metamolecules sensitive toboth incident electric and magnetic fields with stronger induced magnetic dipolemoment upon excitation in comparison to planar SRRs. The fabrication techniqueenabled us to study the plasmon coupling between VSRRs that stand up side by sidewhere the coupling strength can be precisely controlled by varying the gap inbetween. The resulting wide tuning range of these resonance modes offers thepossibility of developing frequency selective functional devices such as sensors andfilters based on plasmon coupling with high sensitivity.
Enhanced renoprotective effect of HIF-1α modified human adipose-derived stem cells on cisplatin-induced acute kidney injury in vivo
Authors: Wang Wei-Wei , Li Ze-Zheng , Wang Wei , Jiang Yan , Cheng Jin , Lu Shi , Zhang Jin-Yuan
Published: June 2015

Current therapeutic options for acute kidney injury (AKI) are limited to the use of supportive measures and dialysis. A recent approach that has sparked great interest and gained enormous popularity is the implantation of stem cells to repair acutely damaged kidney organ. Hypoxia inducible factor-1α (HIF-1α) is effective in protecting the kidney from ischemia and nephrotoxicity. In this study, we investigated whether HIF-1α-modified adipose-derived stem cells (ASCs) had an enhanced protective effect on cisplatin-induced kidney injury in vivo. Cisplatin-induced AKI was established in nude mice. Our study demonstrated that HIF-1α-modified ASCs obviously promoted the recovery of renal function, ameliorated the extent of histologic injury and reduced renal apoptosis and inflammation, but HIF-1α-modified ASCs homed to kidney tissues at very low levels after transplantation. In addition, we also found that HIF-1α-modified ASCs significantly increased HO-1 expression in cisplatin-induced AKI in vivo. Thus, our study indicated HIF-1α-modified ASCs implantation could provide advanced benefits in the protection again AKI, which will contribute to developing a new therapeutic strategy for the treatment of AKI.
The HAB1 PP2C is inhibited by ABA-dependent PYL10 interaction
Authors: Li Juan , Shi Chaowei , Sun Demeng , He Yao , Lai Chaohua , Lv Pei , Xiong Ying , Zhang Longhua , Wu Fangming , Tian Changlin
Published: June 2015

PYL10 is a monomeric abscisic acid (ABA) receptor that inhibits protein phosphatase 2C (PP2C) activity in Arabidopsis thaliana. Previous studies reported that the PP2C phosphatase inhibition by PYL10 was ABA-independent. Here, systematic PYL10 biochemical studies demonstrated that PYL10 activity was ABA-dependent and the previously reported studies was interfered by the presence of BSA in the commercial kit. To investigate dynamic mechanism of how ABA binding to PYL10 induces PP2C phosphatase inhibiting activity, solution NMR relaxation analysis of apo-PYL10 and PYL10/ABA were conducted following backbone resonance assignments. Reduced spectrum density mapping of the backbone relaxation data revealed that PYL10 was more flexible in ABA bound form than apo-PYL10, indicating an increased conformational entropy upon ligand binding. Moreover, to illustrate conformation exchanges of PYL10 upon ABA binding, NMR line shape analysis was performed with increasing concentrations of ABA and the results indicated that PYL10 backbone conformational changes occur at different time scales.
The pericyte as a cellular regulator of penile erection and a novel therapeutic target for erectile dysfunction
Authors: Yin Guo Nan , Das Nando Dulal , Choi Min Ji , Song Kang-Moon , Kwon Mi-Hye , Ock Jiyeon , Limanjaya Anita , Ghatak Kalyan , Kim Woo Jean , Hyun Jae Seog , Koh Gou Young , Ryu Ji-Kan , Suh Jun-Kyu
Published: June 2015

Pericytes are known to play critical roles in vascular development and homeostasis. However, the distribution of cavernous pericytes and their roles in penile erection is unclear. Herein we report that the pericytes are abundantly distributed in microvessels of the subtunical area and dorsal nerve bundle of mice, followed by dorsal vein and cavernous sinusoids. We further confirmed the presence of pericytes in human corpus cavernosum tissue and successfully isolated pericytes from mouse penis. Cavernous pericyte contents from diabetic mice and tube formation of cultured pericytes in high glucose condition were greatly reduced compared with those in normal conditions. Suppression of pericyte function with anti-PDGFR-β blocking antibody deteriorated erectile function and tube formation in vivo and in vitro diabetic condition. In contrast, enhanced pericyte function with HGF protein restored cavernous pericyte content in diabetic mice and significantly decreased cavernous permeability in diabetic mice and in pericytes-endothelial cell co-culture system, which induced significant recovery of erectile function. Overall, these findings showed the presence and distribution of pericytes in the penis of normal or pathologic condition and documented their role in the regulation of cavernous permeability and penile erection, which ultimately explore novel therapeutics of erectile dysfunction targeting pericyte function.
Role of NLRP3 Inflammasomes for Rhabdomyolysis-induced Acute Kidney Injury
Authors: Komada Takanori , Usui Fumitake , Kawashima Akira , Kimura Hiroaki , Karasawa Tadayoshi , Inoue Yoshiyuki , Kobayashi Motoi , Mizushina Yoshiko , Kasahara Tadashi , Taniguchi Shun’ichiro , Muto Shigeaki , Nagata Daisuke , Takahashi Masafumi
Published: June 2015

Rhabdomyolysis is one of the main causes of community-acquired acute kidney injury (AKI). Although inflammation is involved in the pathogenesis of rhabdomyolysis-induced AKI (RIAKI), little is known about the mechanism that triggers inflammation during RIAKI. Recent evidence has indicated that sterile inflammation triggered by tissue injury can be mediated through multiprotein complexes called the inflammasomes. Therefore, we investigated the role of NLRP3 inflammasomes in the pathogenesis of RIAKI using a glycerol-induced murine rhabdomyolysis model. Inflammasome-related molecules were upregulated in the kidney of RIAKI. Renal tubular injury and dysfunction preceded leukocyte infiltration into the kidney during the early phase of RIAKI and they were markedly attenuated in mice deficient in NLRP3, ASC, caspase-1 and interleukin (IL)-1β compared with those in wild-type mice. No difference in leukocyte infiltration was observed between wild-type and NLRP3-deficient mice. Furthermore, NLRP3 deficiency strikingly suppressed the expression of renal injury markers and inflammatory cytokines and apoptosis of renal tubular cells. These results demonstrated that NLRP3 inflammasomes contribute to inflammation, apoptosis and tissue injury during the early phase of RIAKI and provide new insights into the mechanism underlying the pathogenesis of RIAKI.
An effective plasma membrane proteomics approach for small tissue samples
Authors: Smolders Katrien , Lombaert Nathalie , Valkenborg Dirk , Baggerman Geert , Arckens Lutgarde
Published: June 2015

Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters and this from mm3-scale tissue.
All-optical coherent population trapping with defect spin ensembles in silicon carbide
Authors: Zwier Olger V. , O’Shea Danny , Onur Alexander R. , van der Wal Caspar H.
Published: June 2015

Divacancy defects in silicon carbide have long-lived electronic spin states and sharp optical transitions. Because of the various polytypes of SiC, hundreds of unique divacancies exist, many with spin properties comparable to the nitrogen-vacancy center in diamond. If ensembles of such spins can be all-optically manipulated, they make compelling candidate systems for quantum-enhanced memory, communication and sensing applications. We report here direct all-optical addressing of basal plane-oriented divacancy spins in 4H-SiC. By means of magneto-spectroscopy, we fully identify the spin triplet structure of both the ground and the excited state and use this for tuning of transition dipole moments between particular spin levels. We also identify a role for relaxation via intersystem crossing. Building on these results, we demonstrate coherent population trapping -a key effect for quantum state transfer between spins and photons- for divacancy sub-ensembles along particular crystal axes. These results, combined with the flexibility of SiC polytypes and device processing, put SiC at the forefront of quantum information science in the solid state.
L-citrulline for protection of endothelial function from ADMA–induced injury in porcine coronary artery
Authors: Xuan Chao , Lun Li-Min , Zhao Jin-Xia , Wang Hong-Wei , Wang Jue , Ning Chun-Ping , Liu Zhen , Zhang Bei-Bei , He Guo-Wei
Published: June 2015

Endogenous nitric oxide synthase (eNOS) inhibitor asymmetric dimethylarginine (ADMA) is a cardiovascular risk factor. We tested the hypothesis that L-citrulline may ameliorate the endothelial function altered by ADMA in porcine coronary artery (PCA). Myograph study for vasorelaxation, electrochemical measurement for NO, RT-PCR and Western blot analysis for expression of eNOS, argininosuccinate synthetase (ASS) and p-eNOSser1177 were performed. cGMP was determined by enzyme immunoassay. Superoxide anion (O2.−) production was detected by the lucigenin-enhanced chemiluminescence method. Compare with controls (96.03% ± 6.2%), the maximal relaxation induced by bradykinin was significantly attenuated (61.55% ± 4.8%, p < 0.01) and significantly restored by L-citrulline (82.67 ± 6.4%, p < 0.05) after 24 hours of ADMA exposure. Expression of eNOS, p-eNOSser1177 and ASS in PCA significantly increased after L-citrulline incubation. L-citrulline also markedly restored the NO production and cGMP level which was reduced by ADMA. The increased O2.− production by ADMA was also inhibited by L-citrulline. L-citrulline restores the endothelial function in preparations treated with ADMA by preservation of NO production and suppression of O2.− generation. Preservation of NO is attributed to the upregulation of eNOS expression along with activation of p-eNOSser1177. L-citrulline improves endothelium-dependent vasodilation through NO/ cGMP pathway.
Unidirectional thermal expansion in edge-sharing BO4 tetrahedra contained KZnB3O6
Authors: Lou Yanfang , Li Dandan , Li Zhilin , Jin Shifeng , Chen Xiaolong
Published: June 2015

Borates are among a class of compounds that exhibit rich structural diversity and find wide applications. The formation of edge-sharing (es-) BO4 tetrahedra is extremely unfavored according to Pauling’s third and fourth rules. However, as the first and the only es-borate obtained under ambient pressure, es-KZnB3O6 shows an unexpected high thermal stability up to its melting point. The origin of this extraordinary stability is still unclear. Here, we report a novel property in KZnB3O6: unidirectional thermal expansion, which plays a role in preserving es-BO4 from disassociation at elevated temperatures. It is found that this unusual thermal behavior originates from cooperative rotations of rigid groups B6O12 and Zn2O6, driven by anharmonic thermal vibrations of K atoms. Furthermore, a detailed calculation of phonon dispersion in association with this unidirectional expansion predicts the melting initiates with the breakage of the link between BO3 and es-BO4. These findings will broaden our knowledge of the relationship between structure and property and may find applications in future.
A new strategy for controlling invasive weeds: selecting valuable native plants to defeat them
Authors: Li Weihua , Luo Jianning , Tian Xingshan , Soon Chow Wah , Sun Zhongyu , Zhang Taijie , Peng Shaolin , Peng Changlian
Published: June 2015

To explore replacement control of the invasive weedIpomoea cairica, we studied the competitive effects of two valuable natives,Pueraria lobataandPaederia scandens, on growth and photosynthetic characteristics ofI. cairica, in pot and field experiments. WhenI. cairicawas planted in pots withP. lobataorP. scandens, its total biomass decreased by 68.7% and 45.8% and its stem length by 33.3% and 34.1%, respectively. The two natives depressed growth of the weed by their strong effects on its photosynthetic characteristics, including suppression of leaf biomass and the abundance of the CO2-fixing enzyme RUBISCO. The field experiment demonstrated that sowing seeds ofP. lobataorP. scandensin plots where the weed had been largely cleared produced 11.8-fold or 2.5-fold as much leaf biomass of the two natives, respectively, as the weed. Replacement control by valuable native species is potentially a feasible and sustainable means of suppressingI. cairica.
A novel Monoclonal Antibody against Notch1 Targets Leukemia-associated Mutant Notch1 and Depletes Therapy Resistant Cancer Stem Cells in Solid Tumors
Authors: Sharma Ankur , Gadkari Rupali A , Ramakanth Satthenapalli V , Padmanabhan Krishnanand , Madhumathi Davanam S , Devi Lakshmi , Appaji Lingappa , Aster Jon C , Rangarajan Annapoorni , Dighe Rajan R
Published: June 2015

Higher Notch signaling is known to be associated with hematological and solid cancers. We developed a potential immunotherapeutic monoclonal antibody (MAb) specific for the Negative Regulatory Region of Notch1 (NRR). The MAb604.107 exhibited higher affinity for the “Gain-of-function” mutants of Notch1 NRR associated with T Acute lymphoblastic Leukemia (T-ALL). Modeling of the mutant NRR with 12 amino-acid insertion demonstrated “opening” resulting in exposure of the S2-cleavage site leading to activated Notch1 signaling. The MAb, at low concentrations (1–2 μg/ml), inhibited elevated ligand-independent Notch1 signaling of NRR mutants, augmented effect of Thapsigargin, an inhibitor of mutant Notch1, but had no effect on the wild-type Notch1. The antibody decreased proliferation of the primary T-ALL cells and depleted leukemia initiating CD34/CD44 high population. At relatively high concentrations, (10–20 μg/ml), the MAb affected Notch1 signaling in the breast and colon cancer cell lines. The Notch-high cells sorted from solid-tumor cell lines exhibited characteristics of cancer stem cells, which were inhibited by the MAb. The antibody also increased the sensitivity to Doxorubucinirubicin. Further, the MAb impeded the growth of xenografts from breast and colon cancer cells potentiated regression of the tumors along with Doxorubucin. Thus, this antibody is potential immunotherapeutic tool for different cancers.
Signal honesty and predation risk among a closely related group of aposematic species
Authors: María Arenas Lina , Walter Dominic , Stevens Martin
Published: June 2015

Many animals have bright colours to warn predators that they have defences and are not worth attacking. However, it remains unclear whether the strength of warning colours reliably indicate levels of defence. Few studies have unambiguously established if warning signals are honest and have rarely considered predator vision or conspicuousness against the background. Importantly, little data exists either on how differences in signal strength translate into survival advantages. Ladybirds exhibit impressive variation in coloration both among and within species. Here we demonstrate that different levels of toxicity exist among and within ladybird species and that signal contrast against the background is a good predictor of toxicity, showing that the colours are honest signals. Furthermore, field experiments with ladybird models created with regards to predator vision show that models with lower conspicuousness were attacked more frequently. This provides one of the most comprehensive studies on signal honesty in warning coloration to date.
The Evolutionary History of R2R3-MYB Proteins Across 50 Eukaryotes: New Insights Into Subfamily Classification and Expansion
Authors: Du Hai , Liang Zhe , Zhao Sen , Nan Ming-Ge , Tran Lam-Son Phan , Lu Kun , Huang Yu-Bi , Li Jia-Na
Published: June 2015

R2R3-MYB proteins (2R-MYBs) are one of the main transcription factor families in higher plants. Since the evolutionary history of this gene family across the eukaryotic kingdom remains unknown, we performed a comparative analysis of 2R-MYBs from 50 major eukaryotic lineages, with particular emphasis on land plants. A total of 1548 candidates were identified among diverse taxonomic groups, which allowed for an updated classification of 73 highly conserved subfamilies, including many newly identified subfamilies. Our results revealed that the protein architectures, intron patterns and sequence characteristics were remarkably conserved in each subfamily. At least four subfamilies were derived from early land plants, 10 evolved from spermatophytes and 19 from angiosperms, demonstrating the diversity and preferential expansion of this gene family in land plants. Moreover, we determined that their remarkable expansion was mainly attributed to whole genome and segmental duplication, where duplicates were preferentially retained within certain subfamilies that shared three homologous intron patterns (a, b and c) even though up to 12 types of patterns existed. Through our integrated distributions, sequence characteristics and phylogenetic tree analyses, we confirm that 2R-MYBs are old and postulate that 3R-MYBs may be evolutionarily derived from 2R-MYBs via intragenic domain duplication.
Characterization of Adelphocoris suturalis (Hemiptera: Miridae) Transcriptome from Different Developmental Stages
Authors: Tian Caihong , Tek Tay Wee , Feng Hongqiang , Wang Ying , Hu Yongmin , Li Guoping
Published: June 2015

Adelphocoris suturalis is one of the most serious pest insects of Bt cotton in China, however its molecular genetics, biochemistry and physiology are poorly understood. We used high throughput sequencing platform to perform de novo transcriptome assembly and gene expression analyses across different developmental stages (eggs, 2nd and 5th instar nymphs, female and male adults). We obtained 20 GB of clean data and revealed 88,614 unigenes, including 23,830 clusters and 64,784 singletons. These unigene sequences were annotated and classified by Gene Ontology, Clusters of Orthologous Groups and Kyoto Encyclopedia of Genes and Genomes databases. A large number of differentially expressed genes were discovered through pairwise comparisons between these developmental stages. Gene expression profiles were dramatically different between life stage transitions, with some of these most differentially expressed genes being associated with sex difference, metabolism and development. Quantitative real-time PCR results confirm deep-sequencing findings based on relative expression levels of nine randomly selected genes. Furthermore, over 791,390 single nucleotide polymorphisms and 2,682 potential simple sequence repeats were identified. Our study provided comprehensive transcriptional gene expression information for A. suturalis that will form the basis to better understanding of development pathways, hormone biosynthesis, sex differences and wing formation in mirid bugs.
DNA double-strand breaks alter the spatial arrangement of homologous loci in plant cells
Authors: Hirakawa Takeshi , Katagiri Yohei , Ando Tadashi , Matsunaga Sachihiro
Published: June 2015

Chromatin dynamics and arrangement are involved in many biological processes in nuclei of eukaryotes including plants. Plants have to respond rapidly to various environmental stimuli to achieve growth and development because they cannot move. It is assumed that the alteration of chromatin dynamics and arrangement support the response to these stimuli; however, there is little information in plants. In this study, we investigated the chromatin dynamics and arrangement with DNA damage in Arabidopsis thaliana by live-cell imaging with the lacO/LacI-EGFP system and simulation analysis. It was revealed that homologous loci kept a constant distance in nuclei of A. thaliana roots in general growth. We also found that DNA double-strand breaks (DSBs) induce the approach of the homologous loci with γ-irradiation. Furthermore, AtRAD54, which performs an important role in the homologous recombination repair pathway, was involved in the pairing of homologous loci with γ-irradiation. These results suggest that homologous loci approach each other to repair DSBs and AtRAD54 mediates these phenomena.