bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrochemical Fabrication and Characterization of Palladium Nanowires in Nanoporous Alumina Templates
Authors: Larsson Alfred , Abbondanza Giuseppe , Linpé Weronica , Carlà Franceso , Mousley Philip , Hetherington Crispin , Lundgren Edvin , Harlow Gary S.
Published: August 2020

A method for the electrochemical synthesis of palladium nanowires, using porous alumina templates with diameters of 25 nm and 40 nm, is presented. Through an electrochemical barrier layer thinning step, pulsed electrodeposition can take place directly into the anodized aluminum; without need for extra removal, pore opening, and metal contact coating steps. A digital oscilloscope is used to record and integrate the current, allowing the efficiency of the electrodeposition to be calculated. We discuss how using a large “off period” allows for the replenishment of the depleted diffusion layer. The nanowires are characterized by using a focused ion beam (FIB) to create cross-sections which can be accessed with a scanning electron microscope (SEM). With grazing-incidence X-ray diffraction (GI-XRD) we find that the nanowires have a slight compressive strain in the direction that they are confined by the pores (0.58% and 0.51% for the 25 nm and 40 nm pores respectively). Knowing the strain state of the nanowires inside the template is of importance for the use of templated nanowires in devices. Further characterization is made using high-resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray spectroscopy (EDS), after removal from the alumina templates.
Thermodynamic Origin of Reaction Non-Uniformity in Battery Porous Electrodes and Its Mitigation
Authors: Wang Fan , Tang Ming
Published: August 2020

The development of non-uniform reaction current distribution within porous electrodes is a ubiquitous phenomenon during battery charging/discharging and frequently controls the rate performance of battery cells. Reaction inhomogeneity in porous electrodes is usually attributed to the kinetic limitation of mass transport within the electrolyte and/or solid electrode phase. In this work, however, we reveal that it is also strongly influenced by the intrinsic thermodynamic behavior of electrode materials, specifically the dependence of the equilibrium potential on the state of charge: the electrode reaction becomes increasingly non-uniform when the slope of the equilibrium potential curve is reduced. We employ numerical simulations and equivalent circuit model to elucidate such a correlation and show that the degree of reaction inhomogeneity and the resultant discharge capacity can be predicted by a dimensionless reaction uniformity number. For electrode materials that have equilibrium potentials insensitive to the state of charge and exhibit significant reaction non-uniformity, we demonstrate several approaches to spatially homogenizing the reaction current inside porous electrodes, including matching the electronic and ionic resistances, introducing graded electronic conductivity and reducing the surface reaction kinetics.
CpG postconditioning after reperfused myocardial infarction is associated with modulated inflammation, less apoptosis, and better left ventricular function
Authors: Duerr Georg Daniel , Wu Shuijing , Schneider Max Lukas , Marggraf Vanessa , Weisheit Christina Katharina , Velten Markus , Verfuerth Luise , Frede Stilla , Boehm Olaf , Treede Hendrik , Dewald Oliver , Baumgarten Georg , Kim Se-Chan
Published: August 2020

Cytosine-phosphate-guanine (CpG) postconditioning seems to mediate inflammation via Toll-like receptor-1 and Toll-like receptor-9 signaling. Enhanced cytokine and chemokine expressions are partly attenuated by IL-10 and matrix metalloproteinase-8 (MMP8) induction, being associated with lower macrophage infiltration and M1-monocyte differentiation. Furthermore, switch from α- to β-MHC and balanced MMP/TIMP expression led to lesser cardiomyocyte apoptosis, smaller scar size, and preserved cardiac function. Data of pharmacological postconditioning have been widely disappointing to date. Our study suggests a new pathway promoting myocardial postconditioning via Toll-like receptor activation.
Beyond oxygen transport: active role of erythrocytes in the regulation of blood flow
Authors: Richardson Kieran J. , Kuck Lennart , Simmonds Michael J.
Published: August 2020

It was classically thought that the function of mammalian red blood cells (RBCs) was limited to serving as a vehicle for oxygen, given the cells’ abundance of cytosolic hemoglobin. Over the past decades, however, accumulating evidence indicates that RBCs have the capacity to sense low-oxygen tensions in hypoxic tissues, and, subsequently, release signaling molecules that influence the distribution of blood flow. The precise mechanisms that facilitate RBC modulation of blood flow are still being elucidated, although recent evidence indicates involvement of 1) adenosine triphosphate, capable of binding to purinergic receptors located on the vascular wall before initiating nitric oxide (NO; a powerful vasodilator) production in endothelial cells, and/or 2) nonvascular NO, which is now known to have several modes of production within RBCs, including an enzymatic process via a unique isoform of NO synthase (i.e., RBC-NOS), which has potential effects on the vascular smooth muscle. The physical properties of RBCs, including their tendency to form three-dimensional structures in low shear flow (i.e., aggregation) and their capacity to elongate in high shear flow (i.e., deformability), are only recently being viewed as mechanotransductive processes, with profound effects on vascular reactivity and tissue perfusion. Recent developments in intracellular signaling in RBCs, and the subsequent effects on the mechanical properties of blood, and blood flow, thus present a vivid expansion on the classic perspective of these abundant cells.
Comparing High-Intensity Interval Training (HIIT) and Continuous Training on Apelin, APJ, NO, and Cardiotrophin-1 in Cardiac Tissue of Diabetic Rats
Authors: Sabouri Mostafa , Norouzi Javad , Zarei Yashar , Sangani Mojtaba Hassani , Hooshmand Moghadam Babak
Published: August 2020

Background and Aims. Exercise activity is an important method for managing type 2 diabetes. This investigation examined the HIIT and continuous training on apelin, APJ receptor, NO, and cardiotrophin-1 in the cardiac tissue of diabetic rats. Methods. The animals were categorized into 3 groups of HIIT, continuous (CO), and control (C) (all animals were sacrificed immediately and 2 days after exercise training period). Rats underwent the treadmill exercise program either HIIT (12 bouts at 90–95% of VO2 max with 60 s rest at 50% of VO2 max) or CO (60–65% VO2 max for 40 min). Protocols performed 5 days per week for 8 weeks. Apelin, APJ receptor, NO, and cardiotrophin-1 protein expressions were measured using the Western blotting method in the left ventricle. Results. Immediately after HIIT and CO exercise protocols, apelin and CT-1 protein showed a significant difference in contrast by the C-0 group (p<0.01). However, NO values were substantially higher in HIIT-0 compared to C-0 and CO-0 groups rats (p<0.01). After two days of exercise protocols, apelin and NO protein showed a significant increase in HIIT and CO groups in contrast to the C animals (p<0.01). Moreover, APJ and CT-1 protein significantly upregulated in CO-2 and HIIT-2 compared to the other groups (p<0.01). Conclusions. This study indicates that exercise training, despite the type, is an efficient method to modify apelin, APJ receptor, NO, and cardiotrophin-1 values in animals with type 2 diabetes.
Stable Balance Adjustment Structure of the Quadruped Robot Based on the Bionic Lateral Swing Posture
Authors: Qin Jian-Hua , Luo Jie , Chuang Kai-Chi , Lan Tian-Syung , Zhang Lie-Ping , Yi Huai-An
Published: August 2020

Aiming at the problem that the stability of the quadruped robot is decreased as its leg momentum is too high, a stable balance adjustment structure of the quadruped robot based on the bionic lateral swing posture is proposed. First, the leg structure of the quadruped robot is improved and designed by using the mechanism of the lateral swing posture of the leg of the hoof animal. Then, the D-H method is used to construct the corresponding leg kinematics model and determine the generalized coordinates of the leg joints in the lateral swing posture. The torque expression of the quadruped robot when it is tilted is established. Based on the differential equation of momentum of the hip joint and its static stability analysis, the static stability conditions in the upright posture and the bionic lateral swing posture are given. Finally, the experimental simulation and comparative analysis of the upright posture and the lateral swing posture of the quadruped robot are proposed by using the Adams virtual prototype technology. The simulation results show that as the angle of lateral swing increases, the peak value of the positive flip torque of the quadruped robot body increases accordingly, while the degree of tilt decreases accordingly, which shows that the bionic lateral swing posture of the quadruped robot has higher static stability than the traditional upright posture. This research provides a technical reference for the design and optimization of the offline continuous gait of the robot and the improvement of stability.
Network Pharmacology to Uncover the Biological Basis of Spleen Qi Deficiency Syndrome and Herbal Treatment
Authors: Wang Xin , Wu Min , Lai Xinxing , Zheng Jiahui , Hu Minghua , Li Yan , Li Shao
Published: August 2020

Spleen qi deficiency (SQD) syndrome is one of the basic traditional Chinese medicine (TCM) syndromes related to various diseases including chronic inflammation and hypertension and guides the use of many herbal formulae. However, the biological basis of SQD syndrome has not been clearly elucidated due to the lack of appropriate methodologies. Here, we propose a network pharmacology strategy integrating computational, clinical, and experimental investigation to study the biological basis of SQD syndrome. From computational aspects, we used a powerful disease gene prediction algorithm to predict the SQD syndrome biomolecular network which is significantly enriched in biological functions including immune regulation, oxidative stress, and lipid metabolism. From clinical aspects, SQD syndrome is involved in both the local and holistic disorders, that is, the digestive diseases and the whole body’s dysfunctions. We, respectively, investigate SQD syndrome-related digestive diseases including chronic gastritis and irritable bowel syndrome and the whole body’s dysfunctions such as chronic fatigue syndrome and hypertension. We found innate immune and oxidative stress modules of SQD syndrome biomolecular network dysfunction in chronic gastritis patients and irritable bowel syndrome patients. Lymphocyte modules were downregulated in chronic fatigue syndrome patients and hypertension patients. From experimental aspects, network pharmacology analysis suggested that targets of Radix Astragali and other four herbs commonly used for SQD syndrome are significantly enriched in the SQD syndrome biomolecular network. Experiments further validated that Radix Astragali ingredients promoted immune modules such as macrophage proliferation and lymphocyte proliferation. These findings indicate that the biological basis of SQD syndrome is closely related to insufficient immune response including decreased macrophage activity and reduced lymphocyte proliferation. This study not only demonstrates the potential biological basis of SQD syndrome but also provides a novel strategy for exploring relevant molecular mechanisms of disease-syndrome-herb from the network pharmacology perspective.
Ethnoecological, Elemental, and Phytochemical Evaluation of Five Plant Species of Lamiaceae in Peshawar, Pakistan
Authors: Sulaiman  , Shah S. M. , Sadaf  , Amin M. , Gul B. , Begum M.
Published: August 2020

The use of medicinal plants as an unconventional health treatment is gaining considerable recognition and popularity worldwide. The current study was designed to inspect five medicinally important species (such as Mentha longifolia (L.) Huds., Mentha piperita L., Mentha spicata L., Ocimum basilicum L., and Rosmarinus officinalis L.) of Lamiaceae, collected from district Peshawar, through ethnoecological, phytochemical, and elemental analyses. Biological spectra expressed that therophytes (60%) were the dominant life-form class, while nanophyll (60%) was the leading class among leaf size. The ethnobotanical profile showed that all the species were medicinal and ornamental (100%) each, while 60% were used in spices. Quantitative analysis for the macro- and microminerals confirmed the presence of 13 elements (C, N, O, Mg, K, P, S, Ca, Al, Si, Fe, Cl, and Na), which were present in varying amounts from species to species. The methanol extract of leaf samples was used for the analysis of phytochemical constituents such as saponins, flavonoids, tannins, terpenoids, phlobatannins, steroids, and anthraquinones. The medicinal potential of these plants was correlated with the presence of these phytochemicals. Due to the presence of active constituents, the plants had high potential in antifungal, antidiuretic, antioxidant, and anti-inflammatory activities.
Experimental Research on Acoustic Emission Characteristics and Felicity Effects during Coal Fatigue Failure under Cyclic Loading
Authors: Yang Yongjie , Xing Luyi
Published: August 2020

In order to study the acoustic emission characteristics and Felicity effect in the process of coal fatigue failure and reveal the internal relationship between the fatigue damage evolution law and the acoustic emission activity, with the help of MTS815.02 electrohydraulic servo rock mechanics test system and PCI‐2 acoustic emission detection and analysis system, a triaxial cycling loading acoustic emission test was carried out on the coal samples. The results show that the higher the upper limit stress is, the more obvious the degree of fatigue damage will be caused by coal samples. At the same time, the more active acoustic emission signal will appear. The coal samples under linear loading are on the initial damage state, and slight fatigue, moderate fatigue, deep fatigue, and ultimate fatigue failure under cyclic loading. The acoustic emission shows the “L‐” type development evolution law in any previous stress level range, while at the last stress level, it shows the obvious “U‐” type development evolution law. The higher the frequency of the cyclic loading is, the higher the rate of initiation and expansion of the microcrack will be, while the more obvious acoustic emission phenomenon will appear. Furthermore, the ringing counting rate is basically the same as that of the energy counting rate. Under triaxial cyclic loading, a shear failure mode that extends along different directions of fracture surface will be presented. The acoustic emission in the range of different stress levels shows a different degree of Felicity effect. In contrast, it is more reasonable to use the principal stress difference as a parameter to study the Felicity effect of coal under cyclic loading.
Uncovering the Molecular Mechanism of the Qiang-Xin 1 Formula on Sepsis-Induced Cardiac Dysfunction Based on Systems Pharmacology
Authors: He Shasha , Zhao Jingxia , Xu Xiaolong , Cui Xuran , Wang Ning , Han Xuyang , Guo Yuhong , Liu Qingquan
Published: August 2020

Cardiac dysfunction is a critical manifestation of sepsis-induced multiorgan failure and results in the high mortality of sepsis. Our previous study demonstrated that a traditional Chinese medicine formula, Qiang-Xin 1 (QX1), ameliorates cardiac tissue damage in septic mice; however, the underlying pharmacology mechanism remains to be elucidated. The present study was aimed at clarifying the protective mechanism of the QX1 formula on sepsis-induced cardiac dysfunction. The moderate sepsis model of mice was established by cecal ligation and puncture surgery. Treatment with the QX1 formula improved the 7-day survival outcome, attenuated cardiac dysfunction, and ameliorated the disruption of myocardial structure in septic mice. Subsequent systems pharmacology analysis found that 63 bioactive compounds and the related 79 candidate target proteins were screened from the QX1 formula. The network analysis showed that the QX1 active components quercetin, formononetin, kaempferol, taxifolin, cryptotanshinone, and tanshinone IIA had a good binding activity with screened targets. The integrating pathway analysis indicated the calcium, PI3K/AKT, MAPK, and Toll-like receptor signaling pathways may be involved in the protective effect of the QX1 formula on sepsis-induced cardiac dysfunction. Further, experimental validation showed that the QX1 formula inhibited the activity of calcium/calmodulin-dependent protein kinase II (CaMKII), MAPK (P38, ERK1/2, and JNK), and TLR4/NF-κB signaling pathways but promoted the activation of the PI3K/AKT pathway. A cytokine array found that the QX1 formula attenuated sepsis-induced upregulated levels of serum IFN-γ, IL-1β, IL-3, IL-6, IL-17, IL-4, IL-10, and TNF-α. Our data suggested that QX1 may represent a novel therapeutic strategy for sepsis by suppressing the activity of calcium, MAPK, and TLR4/NF-κB pathways, but promoting the activation of AKT, thus controlling cytokine storm and regulating immune balance. The present study demonstrated the multicomponent, multitarget, and multipathway characteristics of the QX1 formula and provided a novel understanding of the QX1 formula in the clinical application on cardiac dysfunction-related diseases.
High Efficiency and Problems of Chemiluminescence Assay-Detected Aldosterone-To-Renin Ratio in Practical Primary Aldosteronism Screening
Authors: Lin Wenbin , Li Yuzhe , Chen Dubo , Yao Zhenrong , Xu Hongxu , Chen Yonghong , Xiao Jiahao , Feng Pinning , Gan Wenjia
Published: August 2020

Primary aldosteronism is a main cause of secondary hypertension which can be effectively treated. The screening test for primary aldosteronism is benefit for minimizing damage to the patient. In the previous retrospective study, we obtained the optimal cutoff value of aldosterone-to-renin ratio detected by chemiluminescence assay, a newly developing method, and prompted its high efficiency in primary aldosteronism screening in upright position. In this study, we want to evaluate its efficiency in practical work. We used this ratio to continuously screen 238 patients, and 58 patients were finally diagnosed with primary aldosteronism. We found it had 86.13% accuracy rate in the upright position compared with the final clinical diagnosis. False negative and positive rates were 13.79% and 13.89%. Diagnostic sensitivity and specificity were 86.21% and 86.11%, which are slightly different from results in our previous study. False negative rate can be improved by combining the aldosterone-to-renin ratio with aldosterone concentration. We also found impaired glucose tolerance may be a reason for high false positive rate. Besides, chemiluminescence assay may be interfered in aldosterone detection. Although it has some shortcomings, chemiluminescence assay-detected aldosterone-to-renin ratio is a highly effective index for screening primary aldosteronism in practice.
Estimating the Aboveground Biomass of an Evergreen Broadleaf Forest in Xuan Lien Nature Reserve, Thanh Hoa, Vietnam, Using SPOT-6 Data and the Random Forest Algorithm
Authors: Nguyen The Dung , Kappas Martin
Published: August 2020

Forest biomass is an important ecological indicator for the sustainable management of forests. The aim of this study was to estimate forest aboveground biomass (AGB) by integrating SPOT-6 data with field-based measurements using the random forest (RF) algorithm. In total, 52 remote sensing variables, including spectral bands, vegetation indices, topography data, and textures, were extracted from SPOT-6 images to predict the forest AGB of Xuan Lien Nature Reserve, Vietnam. To determine the optimal predictor variables for AGB estimation, 10 different RF models were built. To evaluate these models, 10-fold cross-validation was applied. We found that a combination of spectral and vegetation indices and topography variables offer the highest prediction results (Radj2  = 0.74 and RMSE = 61.24 Mg ha−1). Adding texture features into the predictor variables did not improve the model performance. In addition, the SPOT-6 sensor has the potential to predict forest AGB using the RF algorithm.
Concordance of Genomic Alterations between Circulating Tumor DNA and Matched Tumor Tissue in Chinese Patients with Breast Cancer
Authors: Xu Bing , Shan Guangyu , Wu Qixi , Li Weiwei , Wang Hongjiang , Li Hui , Yang Yaping , Long Qiming , Zhao Ping
Published: August 2020

Purpose. Circulating tumor DNA (ctDNA) served as a noninvasive method with less side effects using peripheral blood. Given the studies on concordance rate between liquid and solid biopsies in Chinese breast cancer (BC) patients were limited, we sought to examine the concordance rate of different kinds of genomic alterations between paired tissue biopsies and ctDNA samples in Chinese BC cohorts. Materials and Methods. In this study, we analyzed the genomic alteration profiles of 81 solid BC samples and 41 liquid BC samples. The concordance across 136 genes was evaluated. Results. The median mutation counts per sample in 41 ctDNA samples was higher than the median in 81 tissue samples (p=0.0254; Wilcoxon rank sum test). For mutation at the protein-coding level, 39.0% (16/41) samples had at least one concordant mutation in two biopsies. 20.0% tissue-derived mutations could be detected via ctDNA-based sequencing, whereas 11.7% ctDNA-derived mutations could be found in paired tissues. At gene amplification level, the overall concordant rate was 68.3% (28/41). The concordant rate at gene level for each patient ranged from 83.8% (114/136) to 99.3% (135/136). And, the mean level of variant allele frequency (VAF) for concordant mutations in ctDNA was statistically higher than that for the discordant ones (p<0.001; Wilcoxon rank sum test). Across five representative genes, the overall sensitivity and specificity were 49.0% and 85.9%, respectively. Conclusion. Our results indicated that ctDNA could provide complementary information on genetic characterizations in detecting single nucleotide variants (SNVs) and insertions and deletions (InDels).
DFT Study of Cs/Sr/Ag Adsorption on Defective Matrix Graphite
Authors: Jiao Zengtong , Chen Xiaotong , Fang Chao , Xu Gang , Zhang Chi , Fan Luhao , Liu Bing
Published: August 2020

The geometries, adsorption energies, and electronic structures of Cs, Sr, and Ag atoms on matrix graphite surface with point defects were calculated and analyzed using the density functional theory (DFT) and the Perdew–Burke–Ernzerhof (PBE) formulation of the generalized gradient approximation (GGA). Three different types of point defects, i.e., single vacancy and “bridge” and “spiro” interstitials are considered using approximate van der Waals (vdW) correction methods. The results of adsorption energies show that the metal fission products of Cs, Sr, and Ag are more stable on single vacancy defects than “bridge” or “spiro” interstitial defects. This is further confirmed by the analysis of electronic structures, such as charge density difference (CDD) and density of state (DOS). All these results indicate that dangling bonds play an important role in the adsorption behaviors of metallic fission products on matrix graphite.
Spatial Distribution Prediction of Oil and Gas Based on Bayesian Network with Case Study
Authors: Ren Hongjia , Wang Xianchang , Ren Hongbo , Guo Qiulin
Published: August 2020

Effectively predicting the spatial distribution of oil and gas contributes to delineating promising target areas for further exploration. Determining the location of hydrocarbon is a complex and uncertain decision problem. This paper proposes a method for predicting the spatial distribution of oil and gas resource based on Bayesian network. In this method, qualitative dependency relationship between the hydrocarbon occurrence and key geologic factors is obtained using Bayesian network structure learning by integrating the available geoscience information and the current exploration results and then using Bayesian network topology structure to predict the probability of hydrocarbon occurrence in the undiscovered area; finally, the probability map of hydrocarbon-bearing is formed by interpolation method. The proposed method and workflow are further illustrated using an example from the Carboniferous Huanglong Formation (C2hl) in the eastern part of the Sichuan Basin in China. The prediction results show that the coincidence rate between the results of 248 known exploration wells and the predicted results reaches 89.5%, and it has been found that the gas fields are basically located in the high value area of the hydrocarbon-bearing probability map. The application results show that the Bayesian network method can effectively predict the spatial distribution of oil and gas resources, thereby reducing exploration risks, optimizing exploration targets, and improving exploration benefits.
Evaluation of Particulate Matter Capture and Long‐Term Clogging Characteristics of Different Filter Media for Pavement Runoff Treatment
Authors: Wu Zhengguang , Qi Yanjuan , Kang Aihong , Li Bo , Xu Xueling
Published: August 2020

The removal of particulate matter (PM) by filter media during filtration process can mitigate the pavement runoff pollution effectively. However, this process also makes the filter media prone to clogging. To better understand the size ranges of PM captured by filter media and the subsequent impact on the clogging process, filtration test and clogging test were conducted on five types of filter media. The effect of layer thickness and grain size of different filter media on particle removal efficiency was evaluated, based on the results of PM removal rate and the particle size distribution. The subsequent long‐term clogging characteristics of different filter media were also investigated. The results showed that filter media presented different capabilities to capture PM, which could be enhanced by less layer thickness or finer grain size. Normally, PM with the size range of over 49 μm could be captured effectively if proper layer thickness and grain size of filter media were selected. Besides, PM removal rate was not related to the clogging resistance of filter media. Though it can capture a larger amount of PM, Vesuvianite still maintained remarkable clogging resistance. The results will be beneficial to filter media selection and system design optimization for pavement runoff treatment.
The Existence of Positive Solution of a Nonlinear Problem on Unit Circle
Authors: Wei Na , Zhou Yanli , Ge Xiangyu
Published: August 2020

We consider the following nonlinear problem u″x+λux+fx,u=0, x∈S1, (P) where S1 is a unit circle, λ∈ℝ is a parameter, and fx,u is a nonlinearity. By using the variational method, we obtain the existence of a positive solution to (P) for all λ∈ℝ. This phenomenon is different from the corresponding boundary value problem.
EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic db/db Mice and HK-2 Cells
Authors: Yang Xiu Hong , Zhang Bao Long , Zhang Xiao Meng , Tong Jin Dong , Gu Yan Hong , Guo Li Li , Jin Hui Min
Published: August 2020

To explore whether epigallocatechin-3-gallate (EGCG) improves renal damage in diabetic db/db mice and high-glucose- (HG-) induced injury in HK-2 cells by regulating the level of Klotho gene promoter methylation. Western blotting was used to detect the protein expression levels of DNA methyltransferase 1 (DNMT1), DNMT3a, DNMT3b, transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (α-SMA), and Klotho. The methylation level of the Klotho gene promoter was detected by pyrosequencing. Chromatin immunoprecipitation was used to detect the binding of the Klotho gene promoter to DNMT1 and DNMT3a. The expression of oxidative stress markers (reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and 8-hydroxy-2′-deoxyguanosine (8-OHdG)) and inflammatory cytokines (interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α)) in kidney homogenates was also measured using ELISA. Klotho and DNMT3b protein expression was upregulated, while DNMT1, DNMT3a, TGF-β1, and α-SMA protein expression was downregulated after EGCG treatment. EGCG treatment also reduced the methylation level of the Klotho gene promoter as well as the binding of DNMT3a to the Klotho gene promoter. In addition, EGCG treatment significantly decreased the levels of ROS, MDA, 8-OHdG, IL-1β, IL-6, and TNF-α and increased the levels of CAT and SOD. Under HG conditions, EGCG regulated Klotho gene promoter methylation via DNMT3a and decreased the methylation level of the Klotho gene promoter, thereby upregulating the expression of the Klotho protein to exert its protective effect.
Prevalence and Associated Risk Factors of Trypanosoma evansi in Camels in Ethiopia Based on Parasitological Examinations
Authors: Gerem Bekalu , Hamid Muhammed , Assefa Ayalew
Published: August 2020

Camel trypanosomosis (surra), caused by Trypanosoma evansi , is a life-threatening disease with negative impacts on health, production, and working efficiency of camels in different camel-rearing areas of the world, including Ethiopia. A cross-sectional study was carried out from November 2016 to May 2017 to determine the prevalence of camel trypanosomosis (surra) and assess the associated potential risk factors in Dubti and Asayita districts of Afar region, Ethiopia. Blood samples were collected from 200 camels. Wet film and Giemsa-stained blood smears were used for the detection of trypanosomes. Out of 200 examined, 9(4.5%) were positive for Trypanosoma evansi. The higher prevalence of the disease was observed in Dubti (6.97%) than in Asayita (2.63%) districts. Highest infection was observed in the age group >4 years old camels (7.47%), followed by <4 years old camels (1.07%). Previously aborted camels were found at higher risk (P=0.03; OR = 5.11, 95% CI = 1.174–22.317) than camels without an abortion history. There was no statistically significant difference in between body condition categories and herd size of camels with the occurrence of the disease (X2 = 3.839; P=0.147 and X2 = 0.718; P=0.698), respectively. The result of the current study revealed that camel trypanosomosis is substantially prevalent in the study area, indicating the need for designing control and prevention strategies.
Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials
Authors: Socohou Akim , Sina Haziz , Degbey Cyriaque , Nanoukon Chimène , Chabi-Sika Kamirou , Ahouandjinou Hélène , Lehmane Halfane , Baba-Moussa Farid , Baba-Moussa Lamine
Published: August 2020

Staphylococcus spp. is most often implicated in nosocomial infections. The objective of this study is to evaluate the susceptibility to antibiotics and the biofilm formation capacity of staphylococci species isolated from surfaces and medicotechnical materials at the university hospital center of Abomey-Calavi/Sô-Ava in Benin. Samples were collected according to ISO/DIS14698-1 standard from the surfaces and medicotechnical materials by the dry swab method. The isolation of Staphylococcus strains was performed on Chapman agar, and their identification was performed using microscopic and biochemical methods. The susceptibility of Staphylococcus isolates to antibiotics was evaluated by the disc diffusion method according to EUCAST and CLSI recommendations. The biofilm formation was qualitatively assessed using microplates. Of the 128 surfaces and medicotechnical material samples analyzed, 77% were contaminated with Staphylococcus spp. Thirteen species of Staphylococcus were isolated in different proportions but the pediatric department was the most contaminated (33%) by S. aureus. Resistance to antibiotics considerably varies according to the species of Staphylococcus. However, antibiotics such as chloramphenicol and vancomycin are the most effective on S. aureus, whereas coagulase-negative staphylococci developed less resistance to gentamycin and ciprofloxacin. The biofilm test reveals that 37% of our isolated strains were biofilm formers. Although regular monitoring of hospital hygiene is crucial, the optimal use of antibiotics is a cornerstone of reducing antimicrobial resistance.
Net Assimilation Rate and Agronomic Efficiency of Nitrogen in Tartago (Ricinus communis L.) (Euphorbiaceae) in Dry Climate
Authors: Díaz-López Ernesto , Aguilar-Luna Jesús M. E. , Loeza-Corte Juan M.
Published: August 2020

To know the dynamics of net assimilation rate and the agronomic efficiency of nitrogen in the Tartago crop, seeds of three accessions were collected in Teotitlán de Flores Magón, Oaxaca, Mexico. The treatments consisted of nitrogen fertilization of 0, 20, 40, 60, 80, 100, 120, and 140 kg (N) ha−1, evaluated under a completely randomized design. The experimental unit was constituted by a Tartago plant inside of a polyethylene bag with soil of the zone, and four repetitions were considered. The response variables were dry biomass, number of fruits per plant, agronomic yield, harvest index, nitrogen agronomic efficiency, SPAD units, and net assimilation rate. The results indicate that climatic conditions did not influence the growth and development of the crop. The maximum values for all of response variables were achieved with the application of nitrogen in a range of 60 to 140 kg ha−1. The net assimilation rate was adjusted to a quadratic model. It is concluded that the Tartago responds positively to the application of nitrogen and can be an alternative to be grown in dry climate.
MG53 Protects against Sepsis-Induced Myocardial Dysfunction by Upregulating Peroxisome Proliferator-Activated Receptor-α
Authors: Han Xue , Chen Daili , Liufu Ning , Ji Fengtao , Zeng Qingshi , Yao Weifeng , Cao Minghui
Published: August 2020

Background. The heart is one of the most commonly affected organs during sepsis. Mitsugumin-53 (MG53) has attracted attention in research due to its cardioprotective function. However, the role of MG53 in sepsis-induced myocardial dysfunction (SIMD) remains unknown. The purpose of this study was to explore the underlying mechanism of MG53 in SIMD and investigate its potential relationship with peroxisome proliferator-activated receptor-α (PPARα). Methods. The cecal ligation and puncture (CLP) model was created to induce SIMD in rats. Protein levels of MG53 and PPARα, cardiac function, cardiomyocyte injury, myocardial oxidative stress and inflammatory indicators, and cardiomyocyte apoptosis were measured at 18 h after CLP. The effects of MG53 on PPARα in SIMD were investigated via preconditioning recombinant human MG53 (rhMG53) and PPARα antagonist GW6471. Results. The expression of MG53 and PPARα sharply decreased in the myocardium at 18 h after CLP. Compared with the sham group, cardiac function was significantly depressed, which was associated with the destructed myocardium, upregulated oxidative stress indicators and proinflammatory cytokines, and excessive cardiomyocyte apoptosis in the CLP group. Supplementation with rhMG53 enhanced myocardial MG53, increased the survival rate with improved cardiac function, and reduced oxidative stress, inflammation, and myocardial apoptosis, which were associated with PPARα upregulation. Pretreatment with GW6471 abolished the abovementioned protective effects induced by MG53. Conclusions. Both MG53 and PPARα were downregulated after sepsis shock. MG53 supplement protects the heart against SIMD by upregulating PPARα expression. Our results provide a new treatment strategy for SIMD.
Gender Difference on the Effect of Omega-3 Polyunsaturated Fatty Acids on Acetaminophen-Induced Acute Liver Failure
Authors: Liu Yunzhi , Chen Yu , Xie Xinghuan , Yin Aiping , Yin Yue , Liu Yan , Dong Lijun , Zhu Zhengyumeng , Zhou Jia , Zeng Qingchun , Lu Xiao , Chen Zhengliang , Wen Kun , Zuo Daming
Published: August 2020

Acetaminophen (APAP) toxicity is the leading cause of drug-induced liver failure, which is closely related to mitochondrial dysfunction and oxidative damage. Studies in clinical trials and in animal models have shown that omega-3 polyunsaturated fatty acids (n-3 PUFAs) affect the progression of various types of liver damage. Interestingly, the sex-dependent effect of n-3 PUFAs on human health has also been well documented. However, it is unknown whether supplementation of n-3 PUFAs modulates the pathogenesis of APAP-induced liver failure with sex-specificity. Our results showed that both endogenous and exogenous n-3 PUFAs significantly aggravated the APAP-induced liver injury in male mice, whereas the opposite effects were observed in females. In vivo and in vitro studies demonstrated that estrogen contributes to the gender difference in the regulation of n-3 PUFAs on APAP overdose. We found that n-3 PUFA-mediated regulation of hepatic oxidative stress response and autophagy upon APAP challenge is distinct between male and female mice. Moreover, we provided evidence thatβ-catenin signaling activation is responsible for the sex-dependent regulation of APAP hepatotoxicity by n-3 PUFAs. Together, these findings indicated that supplementation with n-3 PUFAs displays sex-differential effect on APAP hepatotoxicity and could have profound significance in the clinical management for drug-induced liver injury.
Antibacterial Potential of Jatropha sp. Latex against Multidrug-Resistant Bacteria
Authors: Prastiyanto Muhammad Evy , Tama Prayoda Deri , Ananda Ninda , Wilson Wildiani , Mukaromah Ana Hidayati
Published: August 2020

Objective. This study was aimed to evaluate the antibacterial activity of the latex of three species members of Jatropha (J. curcas, J. gossypilofia Linn., and J. multifida) against methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum beta-lactamase- (ESBL-) producing Escherichia coli and ESBL-producing Klebsiella pneumonia, carbapenemase-resistant Enterobacteriaceae (CRE)-E. coli, K. pneumoniae-carbapenemase (KPC), and carbapenemase-resistant Pseudomonas aeruginosa (CRPA). Method. The antibacterial activities were calculated based on the inhibition zones using the Mueller–Hinton agar diffusion method, minimum inhibitory concentration (MIC) using Mueller–Hinton broth in a microdilution method, and minimum bactericidal concentration (MBC) using blood agar plate. Results. The latex of Jatropha showed antibacterial activities against the MRSA and CRPA. All latex of Jatropha appeared to have the antibacterial activities against MRSA and CRPA in the diffusion method (20.4–23.7 mm and 12–15 mm), MIC (0.19–6.25%, and 25%), and MBC (0.39–12.5% and 50%). Phytochemical screening of latex indicated the presence of flavonoids. Conclusions. The latex of J. curcas, J. gossypilofia Linn., and J. multifida has the potential to be developed as antibacterial agents, especially against MRSA and CRPA strain, but further in vivo research and discovery of the mode of its action are required to shed the light on the effects.
A Method for Determining Feasibility of Mining Residual Coal above Out‐Fashioned Goaf under Variable Load: A Case Study
Authors: Guo Yuxia , Yuan Honghui , Deng Xiaogang , Zhang Yujiang , Du Yunlou , Liu Hui , Feng Guorui , Xu Jiali , Zhao Renle , Doan Dao Viet
Published: August 2020

Out‐fashioned goaf is the protective structure for mining the upper residual coal, and its stability is the core problem in mining the upper residual coal. According to the upward mining demand for No. 5 coal seam above the out‐fashioned goaf in Baizi Coal Mine, a new method is proposed to determine the upward mining safety. According to the analysis of the actual situation of the mine, the coal pillar and suspended roof in the out‐fashioned goaf are taken as the objects. Furthermore, a “coal pillar‐suspended roof” system model based on the variable load induced by abutment pressure of upper coal seam mining is established. After the mechanical model was solved, the parameter acquisition method of the model was established. The basic parameters of Baizi Coal Mine were considered to determine the feasibility of mining residual coal above out‐fashioned goaf. And the effects of variable load on the coal pillar and suspended roof stability were analyzed. The results show that the upper No. 5 coal seam in Baizi Coal Mine can be mined safely. Compared to the traditional method, which simplifies all the upper loads to uniform loads, the new method is safer. The system stability of the suspended roof and coal pillar is influenced by “a/L” and “L.” Axial stress curves of the coal pillar and suspended roof appear nearly parabolic with “a/L” varying. Their maximum values are obtained when the “a/L” value is around 0.5∼0.6. In this situation, the combination structure is most easy to to be damaged. The ratio q′/q has a linear relationship with all stresses of the system model. The failure sequence of the system model is determined by analyzing the relationship between the tensile strength of the suspended roof and compressive strength of the coal pillar. This study provides a reference case for coal resources upward mining under similar conditions.
Comparison of Dilution on Eastern Box Turtle (Terrapene carolina carolina) and Marine Toad(Rhinella marinus) Blood Parameters as Measured on a Portable Chemistry Analyzer
Authors: Griffioen John A. , Stowe Devorah M. , Trosclair Macy , Minter Larry J. , Vanetten Chelsey , Harrison Tara M.
Published: August 2020

Biochemical testing is an important clinical tool in evaluating the physiology of reptiles and amphibians. Suitable point of care analyzers can allow for rapid delivery of results, but small patient size can inhibit sufficient sample collection. This study evaluated the utility of sample dilution with sterile distilled water as a means of biochemical evaluation when sample volume is limited. Blood was collected from 12 eastern box turtles (Terrapene carolina carolina) and 12 marine toads(Rhinella marinus) and analyzed via i-STAT CHEM8+ cartridges. Two undiluted samples and two samples diluted 1 : 1 with sterile water were evaluated immediately following collection for each animal in the study. Analytes reported in the diluted samples were limited to glucose, ionized calcium, and total carbon dioxide. The expected dilution ratio value of diluted to undiluted samples was 0.5, of which glucose in both turtles and toads was nearest. Dilution ratio values for ionized calcium, however, were higher than expected in both turtles and toads. Sample dilution is not recommended for most analytes included on the CHEM8+ cartridge due to values occurring outside the limits of detection for the analyzer. Glucose and ionized calcium values obtained on diluted samples should be interpreted with caution but may provide clinical utility in reptile and amphibian patients where sample volume is limited.
Mechanical Behaviors of Natural Sand Soils and Modified Soils in Heavy‐Haul Railway Embankment
Authors: Zhao Yingying , Yang Yang , Ling Xianzhang , Li Guoyu , Gong Weiming
Published: August 2020

The addition of chemical or mechanical materials, such as fibers or stabilizers, is frequently utilized in geotechnical engineering to improve the mechanical properties of problematic soils. In this study, great efforts have been made to obtain insight into the mechanical properties of the natural, fiber, and chemical additive‐stabilized soil in heavy‐haul railway embankment. A series of triaxial compression tests are conducted on the stabilized samples of different preparation conditions, including water content, compaction degree, confining pressure, fiber content, fiber length, stabilizer content, and curing time. Results show that the shear strength of natural soils shows a distinct increase after adding fiber and chemical additive stabilization. The optimum fiber content and length for fiber stabilization are 0.2% and 12 mm, respectively. The initial tangential modulus and failure stress of chemical stabilized samples increase with the increase of additive dosage or curing time. Meanwhile, a brittle characteristic is observed. In the process of determining the reinforcement methods in practical projects, several other considerations are included, such as equipment and time available, especially for stabilized soils. The fiber‐reinforced soils and stabilized soils are efficient for increasing the shear strength and changing of the brittleness character of the heavy‐haul railway embankment. The results of this study could provide a valuable reference for geotechnical engineers dealing with soil problems, especially for the heavy‐haul railway embankment.
Pharmacokinetics of Transdermal Flunixin Meglumine Following a Single Dose in Marine Toads (Rhinella marina)
Authors: Scott Gregory , Louis Meghan M. , Balko Julie A. , Bublitz Claire M. , Troan Brigid V. , Baynes Ronald E. , Smith Dustin , Minter Larry J.
Published: August 2020

Transdermal administration is an important method of pharmacologic drug therapy in amphibians, made possible by their unique skin physiology and permeability. Despite this, there are relatively few studies that investigate transdermal pharmacokinetics in amphibians. The objective of this study was to investigate the pharmacokinetics of transdermal flunixin meglumine applied topically to marine toads (Rhinella marina). Twenty-one adult marine toads were administered flunixin meglumine (3.3 mg/kg) topically on their dorsum and randomized (n = 7/group) to blood collection at two timepoints from the following: 1, 2, 4, 8, 12, and 24 hours (h), using a sparse sampling protocol. Plasma was analyzed by ultra-performance liquid chromatography-tandem mass spectrometry. Samples were analyzed individually and reported as a mean of the samples at each timepoint. The mean peak plasma concentration was 6.31µg/ml, area under the curve was 29.37 μg-h/mL, and elimination half-life was 2.79 h. No adverse effects were noted in any animals. A subset of 12 animals were euthanized at serial timepoints and necropsied. Histopathology of skin and major organs revealed one minimal superficial lesion in a single toad potentially attributable to flunixin meglumine administration; otherwise, no other treatment-related lesions were observed in 11 of 12 toads. A single topical dose of transdermal flunixin meglumine was rapidly absorbed in marine toads in the current study, and peak plasma concentrations exceeded therapeutic ranges established in cattle with no significant pathologic findings.