Entomological surveillance during a major CHIKV outbreak in northwestern São Paulo: insights from São José do Rio Preto
Authors: Banho Cecília Artico , Parra Maisa Carla Pereira , Nascimento Olivia Borghi , Magnani Gabriel Pires , Moraes Ferreira Maria Vitoria , Lemos Ana Paula , de Carvalho Marques Beatriz , Brancini Marini Lino , Sacchetto Livia , Negri Andreia Francesli , Tironi Menezes Regiane Maria , de Deus Juliana Telles , Estofolete Cassia Fernanda , Vasilakis Nikos , Nogueira Maurício Lacerda 
Published: December 2024

Background

Brazil is considered an epicenter for emerging and re-emerging arboviruses that significantly impact public health. The mid-sized city of São José do Rio Preto (SJdRP) in northwestern São Paulo state is considered hyperendemic for arboviral diseases, with case numbers climbing each year. Only 45 cases of chikungunya (CHIKV) were reported in the city from 2015 to 2022, indicating cryptic circulation of this virus, but cases in the state increased notably in 2023. This study investigates the use of active entomological surveillance to detect new arbovirus introductions in specific areas like SJdRP.

Methodology/Principal findings

We used molecular testing to investigate the presence of CHIKV in adult culicids collected monthly from various neighborhoods in SJdRP. Positive samples underwent whole-genome sequencing and phylogenetic analysis. Entomological surveillance successfully detected the early spread of CHIKV across SJdRP, revealing an infection rate of 6.67%, with the well-established vectorsAedes aegyptiandAe. albopictusas well asCulexsp. carrying the virus. The vector positivity rate increased from December 2023 to April 2024, which correlates with rising numbers of chikungunya fever cases reported in SJdRP during the same period. The resurgence of CHIKV in this region is attributed to several introduction events, mainly from the Southeast and North of Brazil, which facilitated establishment of the virus within the highly dense vector population and led to extensive spread and, in turn, a major CHIKV epidemic in this geographical area.

Conclusions/significance

Extensive circulation of CHIKV was documented within the human and vector population, marking the onset of the first major CHIKV epidemic in SJdRP and neighboring cities. Because multiple arboviruses co-circulate in several locations in Brazil, entomological surveillance, along with ongoing monitoring of patient samples, is a key to help health authorities to implement more effective measures to interrupt transmission cycles and mitigate new epidemic waves.

Author summary

The city of São José do Rio Preto (SJdRP) in northwestern São Paulo state is an endemic area for dengue virus (DENV), but cases of chikungunya virus (CHIKV) were also reported between January and September 2023. Since overlapping symptoms between these acute febrile diseases can complicate differential diagnosis, the increase in CHIKV cases in DENV-endemic regions is concerning. Entomological surveillance is a useful strategy for accurate and early detection of arboviruses, making it possible to identify emerging or increased arbovirus activity, predict potential outbreaks, and support effective control measures, thus reducing impacts on public health. Through entomological surveillance we were able to detect the spread of CHIKV in SJdRP, revealing a high infection rate in the vector population. Our findings also suggest that the virus spread widely throughout the local mosquito population, potentially via vertical or sexual transmission, which may have contributed to sustained transmission during unfavorable conditions or inter-epidemic periods. We also observed a monthly increase in the vector population’s positivity rate which correlates with a rise in CHIKV cases in the city and the first CHIKV outbreak in this area.