bluuebunny/crossref_metadata_2025 huggingface dataset:
Literatura Negra Brasileira e os Diálogos com o Cânone: Outros Olhares, Outras Histórias
Authors: ROSANGELA SARTESCHI
Published: July 2024

RESUMO: Este trabalho tem por objetivo refletir sobre aspectos da construção identitária observada em poemas de autoria negra em confronto com a representação desses sujeitos, em uma escrita orientada pelos valores éticos e estéticos fundamentalmente brancos. Busca-se também apontar, nos autores brasileiros escolhidos, uma trajetória de resistência que enseja construir um horizonte utópico em que o negro surge enfim como voz essencial no âmbito do sistema literário nacional. Nesse sentido, problematizar-se-á de que maneira a literatura de autoria negra, muito além de constituir-se na voz dos dominados, ameaça e dissolve a visão hegemônica que simula uma sociedade brasileira homogênea, monocultural e unidimensional.
PALAVRAS-CHAVE: poesia negra brasileira, resistência, utopia
ABSTRACT: This work aims to reflect on aspects of the identity construction observed in poems of black authorship in confrontation with the representation of these subjects, in a script oriented by fundamentally white ethical and aesthetic values. In the chosen Brazilian authors, I also tried to point out a trajectory of resistance that leads to the construction of a utopian horizon in which the black man emerges as an essential voice within the national literary system. In this sense, I will question how the black authorship literature, much as it constitutes the voice of the dominated, threatens and dissolves the hegemonic vision that simulates a homogeneous, monocultural, and unidimensional Brazilian society.
KEYWORDS: Brazilian black poetry, resistance, utopia
Por Formas Amefricanas de Autoinscrição
Authors: ANA FLAUZINA  , THULA PIRES
Published: July 2024

RESUMO: O artigo discute, a partir das noções de racismo antinegro, genocídio e amefricanidade, os processos de assalto à vida negra como o grande sustentáculo do pacto social e político no Brasil. Sustenta que a fragilidade da existência social negra, pertencente à zona do não ser, é condição historicamente delineada pelos processo coloniais. Nesse processo de expropriação, sublinha que há um posicionamento compulsório dos sujeitos negros como os receptores de uma violência gratuita e naturalizada nas órbitas do Estado e da sociedade civil. Destaca ainda que esse padrão perverso tem sido aprofundado no atual contexto político do Brasil, em que se observa um alinhamento institucional com as demandas históricas de produção de extermínios fundamentados pelo racismo. Convoca, por fim, a um tipo de reação que passe pelo reconhecimento de privilégios nos setores ditos progressistas, a fim de que se possa superar as condicionantes do racismo que tem estruturado a tragédia social brasileira. 
PALAVRAS-CHAVE: Genocídio, Amefricanidade, Racismo antinegro
ABSTRACT: The article discusses, from the notions of antiblackness, genocide, and amefricanity, the processes of assault on the black life as the great support of the social and political pact in Brazil. It maintains that the fragility of black social existence, belonging to the zone of non-being, is a condition historically delineated by colonial processes. In this process of expropriation, there is a compulsory positioning of black subjects as the recipients of gratuitous and naturalized violence in the orbits of the state and civil society. It also highlights that this perverse pattern has been deepened in the current political context of Brazil, in which an institutional alignment with the historical demands of production of exterminations based on racism is observed. Finally, it calls for a kind of reaction based on the recognition of privileges in the so-called progressive sectors, in order to overcome the determinants of racism that has shaped the Brazilian social tragedy.
KEYWORDS: Genocide, Amefricanity, Antiblackness racism
Comparison of Cultural Equivalence in Indonesian and German Versions of Show Yourself Songs
Authors: Nareswari Vianey Somya , Rosyidah Rosyidah
Published: July 2024

This study aims to describe the comparison of cultural aspect equivalence in the Indonesian and German translated versions of the song Show Yourself. In this research, a qualitative method is used with datasources in the form of the original song lyrics entitled‘Show Yourself’, the lyrics of the Indonesian translated version entitled Tunjukkan dirimu, and the lyrics of the German translated version entitled Zeige Dich. Meanwhile, the data of this research are words, phrases, clauses, and sentences that are culturally charged in the lyrics of these songs. The technique of ‘Simak Catat’ is an activity of listening and recording existing information from data sources to obtain data. After that, it was analyzed with three stages, namely data reduction, data presentation, and conclusion drawing/verification. The results showed that there are four of the five categories of linguistic forms of cultural aspects proposed by Stolze in the song, namely the concept of terminology, language form, syntax, and pragmatics. In addition, according to Pym's equivalence theory, both translated versions of the song use the concept of directional equivalence, i.e. the translator uses a free strategy in the process of translating the song.
'Into the Unknown': a Comparative Study of Figurative Language in German, Tamil, and English
Authors: Sivan Pavita , Jalis Farhana Muslim Mohd
Published: July 2024

Figurative language is vocabulary that is used creatively, departing from its literal meaning. Song is one of the resources used by language learners to fill in the gaps between one another. This study was done for non-native speakers of foreign languages who might have trouble understanding figurative language. Finding the most common figurative language among the three languages is the goal of this study. Perrine's theory is applied in this study to analyze the kind of figurative language. Furthermore, Tilly's theory was applied to analyze the figurative language's contextual meaning. The types of figurative language were analyzed in this study using a qualitative method. There are 37 types of figurative language from the English version, 22 types from the Tamil version, and 29 types from the German version. The English version is the most frequently used figurative language among the three languages. Therefore, researchers in the future should examine the parallels, differences, and trends in these languages' use of figurative language, according to this study.
POLITICAL AND SOCIO-ECONOMIC VIEWS IN THE PROCESS OF REGIONAL COOPERATION AND GLOBALIZATION IN CENTRAL ASIA
Authors: Berdygulova Gulmira , Erbolat Elay
Published: July 2024

The purpose of this article is to analyze the problems of regional cooperation in Central Asia under the conditions of globalization. The word globalization is usually a characteristic rather than an analytical category. Novelty, directions and ideas of the research. In this regard, the article focuses on positive and normative analysis of globalization. Special attention is paid to trade, environment, finance and gender issues. The preferred situation is a global governance structure based on principles of both economic efficiency and social justice; however, the current disrupted globalization process is likely to end in regionalism or even national protectionism and competition. Multilateral cooperation based on the rationale advanced here is an urgent necessity. For Central Asia, such multilateral regional cooperation poses particular challenges. This article therefore explores possibilities for addressing such challenges. The article uses factor analysis and synthesis methods. The article also analyzes the unique aspects of the interaction of political and economic factors in the context of globalization to identify specific challenges and opportunities for the countries of Central Asia.
STUDY ON THE EVOLUTION OF CHINESE REMINISCENCE POETRY IN ANCIENT AND MODERN TIMES
Authors: Sylam Дина
Published: July 2024

As a literary creation method, reminiscence is often encountered in Chinese and foreign literary works. In the West, there is the theory of "poetic reminiscence", however, the theoretical construction of Chinese reminiscence poetics has not been formed yet. The reason is that the current academic world does not treat reminiscence poetry as a type of poetry. As a matter of fact, Chinese recall poetry has always existed, and it has been spbud since the time of “The Book of Songs”. However, it was not until the Two Jin Dynasties that really formed the poems with the theme of reminiscence. In the Tang and Song dynasties, it was further expanded, which was reflected in the breakthrough of the reminiscence mode of the past and present, and appearance of the emergence of poems with whole reminiscence, at the same time, the suppoter of it expand itself from the poet to the “Ci.”In the modern period of Ming and Qing Dynasties, the emotional expression of reminiscence poetry has changed from sadness to broadness, which has continued to the present and contemporary times, only the traditional contrast mode of past and present has been replaced by narrative reminiscence. Based on the evolution of Chinese reminiscence poetry and the theory of western reminiscence poetics, it can be seen that the reminiscence poetry starts from a real thing we have seen, which gives rise to the reminiscence, then the poet expresses emotions in the traditional mode of past and present, joy and sorrow. Besides, there are also reminiscences of the whole content, mainly with image and narrative recall.
The Kandings: A Study on Sex for Drugs in Iligan City
Authors: Along Jovell Nicole , Labadisos Renebel
Published: July 2024

Drug addiction in the Philippines is still rampant and widely perceived to be the root of poverty, injustice, and powerlessness (Simbulan et al., 2019). This phenomenon gave rise to a multifaceted, overlapping problem. One of these issues is the practice of exchanging sex for drugs, which led to the emergence of Kandings. This study dwells on the case of Iligan City. Drawing concepts from the social exchange theory of George Homans and utilizing a qualitative case study, ten key informants were interviewed through snowball sampling, aged 16 and older. Findings shed light on the origins of the term Kanding as perceived by the informants, a term they believed had its roots primarily in the existence of illegal drug trading in Iligan City. Moreover, the informants represent a disadvantaged group with limited resources, which explains the reasons they manifest experiences as reflected in the typologies of Kanding, mainly the sex for drugs Kanding, the Kanding as an intimate partner, the Kanding as a sex worker, and the Kanding for self-gratification. Despite challenges, including stigma, unresolved transactions, and the acquisition of sexually transmitted infections, they engage in this behavior to obtain access to illegal drugs and earn money with drug users as their typical sexual partners. However, the informants maintained their optimism, wanting the best for their families, their children's success, and their strong faith in God to support them in overcoming these challenges.
Pamakyabe: Acculturation and Basic Psychological Needs Through the Lens of Adolescent Mag-Indi Aetas of Floridablanca, Pampanga
Authors: Susim Paulino
Published: July 2024

This study examined the neglect and discrimination faced by the Mag-Indi Aeta community in Florida Blanca, Pampanga, Philippines, and its impact on their mental health. It also explored the community's adaptive strategies and their implications for cultural preservation, resilience, and basic psychological needs. Using a qualitative research design and interpretative phenomenological analysis, the study investigated the lived experiences of five adolescent Mag-Indi Aetas concerning acculturation and psychological needs. The analysis revealed ten themes: Autonomy in Expression and Interaction, Autonomy in Empowerment Dynamics, Competence in Skill Utilization and Self-Esteem Enhancement, Competence in Modern and Practical Skills, Diverse Acculturation in Values, Diverse Acculturation in Behavior, Relatedness through Varied Engagement Levels, Relatedness through Diverse Emotional Interactions, Diverse Acculturation in Knowledge, and Diverse Acculturation in Cultural Identity. This research highlighted the discrimination, acculturation processes, and psychological needs of indigenous communities, advocating for inclusive and culturally sensitive programs such as self-awareness programs, Usap Tamo benches (buddy benches), IP day, and Kapatad week. These initiatives aim to improve the well-being of indigenous populations, promote cultural exchange, and eliminate discrimination and bullying.
Effects of resin harvesting on the status of the Agathis philippinensis population in the Cleopatra’s Needle Critical Habitat, the Philippines
Authors: Vermeer Lars , Bocxe Kellie , Zuidema Pieter , Sopsop Lita , Hoevenaars Kyra , Reyes Antonio Karina
Published: July 2024

In Palawan, the Philippines, a biological hotspot was turned into a protected area, called Cleopatra’s Needle Critical Habitat (CNCH). The most important goals of the CNCH are to conserve the rich endemic biodiversity and to maintain the culture of the Batak, a group of indigenous people who depend on forest resources for their livelihood. As resin extraction from Agathis philippinensis is a key component of the income of the Batak people, it is important to study the scope for sustainable exploitation of this species. This study focused on the effects of resin harvesting on the physical status and mortality of A. philippinensis trees in 15 subpopulations within the CNCH. These population characteristics were related to the intensity of resin harvest and the distance to communities. We found that the physical tree status deteriorated and the proportion of dead trees increased with harvest intensity and proximity to communities. These results indicate that overharvesting of the resource is taking place, which may lead to prolonged recruitment failure and population decline of A. philippinensis in the study area.
Elysia leucolegnote (Opistobranchia: Sacoglossa) Jensen 1990, a new record for the Verde Island Passage, Philippines
Authors: Sanchez-Escalona Katherine
Published: July 2024

Aggregations of Elysia leucolegnote were observed from Silonay Mangrove and Ecopark, Calapan, Oriental Mindoro, occurring on mudholes with water retained from retreating tide waters. The individuals in the aggregations has white markings on the parapodia and the rhinophores, and yellow marking on each quadrant of the body. This is the first report of the occurrence of the mangrove-associated species from the Philippines which increased the number of Elysia species records to 10 species.
Notes on the first record of Tridacna noae (Röding, 1798) (Cardiidae: Tridacninae) in Palawan, Philippines
Authors: Ecube Krizia Meryl , Villanueva Elmer , Dolorosa Roger , Cabaitan Patrick
Published: July 2024

The first record of Tridacna noae in the province of Palawan, Philippines was documented on June 21, 2018, at Paraiso Resort, Albaguen Island, Port Barton in the municipality of San Vicente. The single specimen measured 4.5 cm in shell length and was partly buried in a massive coral rock. The mantle edge of the species is lined with teardrop-like patches with white margins. This recent finding is an addition to the seven previously reported giant clam species in Palawan and confirms new sighting location in the existing geographic range. The habitat of the species is a semi protected cove, about 1 m deep at high tide, with massive coral rocks generally covered with the seaweed Sargassum spp. Potential threats include the shading effects of macro algae and the constant presence of tourists visiting the resort who might accidentally step on the clams. Buoy demarcation to exclude the area from disturbance may help protect the species and other boring giant clam species. Continued assessment may provide information on the status of T. noae in Palawan and in other parts of the country.
PENGARUH BAURAN PEMASARAN TERHADAP MINAT SISWA MEMILIH PERGURUAN TINGGI DIMASA PANDEMI COVID-19 (Studi Kasus Pada SMA dan SMK di Kab. Pesawaran)
Authors: Sormin Rahma Diani , Sari Sherly Etika , Sasti Rara Dinika , Ruwanda Tri
Published: July 2024

Pendidikan menjadi salah satu jenis investasi jangka panjang yang harus dimiliki oleh seseorang karena dengan pendidikan seseorang dapat menjadi sumber daya manusia yang handal dan mampu bersaing dimasa yang akan datang. Antusias masyarakat Indonesia melanjutkan pendidikan ke perguruan tinggi kian meningkat hingga tahun 2019. Namun dua tahun terahir ini antusias masyarakat untuk melanjutkan pendidikan ke perguruan tinggi mengalami penurunan yang cukup signifikan terutama perguruan tinggi swasta di Lampung. Hal ini disebebkan oleh banyak siwa yang menunda studinya akibat dari pandemi covid-19. Permasalahan ini menjadi ajang perlombaan bagi perguruan tinggi melakukan pemasaran kepada publik agar menarik minat masyarakat untuk menanjutkan pendidikan ke perguruan tinggi yang mereka naungi. Menurut Philip Kholter (2004:9), pemasaran jasa dapat dilakukan dengan menggunakan 7 P yaitu produc, price, promotion, place, people, process dan physical evidence. 7P ini digunakan peneliti sebagai variabel independent. Tujuan  dari penelitian ini ini adalah ingin melihat besaran pengaruh yang diperoleh dari setiap variabel independen. Metode yang digunakan dalam penelitian ini adalah metode asosiatif dengan pendekatan kuantitatif. Hasil penelitian menunjukkan bahwa berdasarkan analisis regresi linear berganda yang telah dilakukan pada penelitian ini, persamaan regresi linear berganda adalah Y = -6.209+0.753 produk + 0.385 harga+ 0.257 promosi+0.115 tempat+0.236 orang+ 0.390 proses+ 0.132 bentuk fisik. Jika di uji secara simultan ternyata ketujuh variabel tersebut memiliki pengaruh signifikan terhadap minat siswa dalam memilih perguruan tinggi dimasa pandemic covid-19 dan besaran pengaruh yang dihasilkan adalah sebesar 0,827 atau 82,7% dan sisanya 17,3% disebabkan oleh faktor lain, dimana faktor lain tersebut tidak di analisis oleh peneliti.Kata Kunci : Pemasaran (7 P), Minat Siswa, Memilih Perguruan Tinggi, Pandemik Covid-19, Kab.Pesawaran.
PENGARUH MEDIA SOSIAL INSTAGRAM TERHADAP PERUBAHAN PERILAKU KOMUNIKASI SECARA LANGSUNG PADA GENERASI Z DI JAKARTA SELATAN
Authors: Aulia Fadhilah Hana  , Wulandari Siti Hardiati , Hasan Basri Made , Fantini Endah
Published: July 2024

The era of technology has given birth to conventional media, one of which is electronic media which plays an important role for humans in communicating through social media. This certainly results in the use of the internet and social media which continues to increase in Indonesia. One of the social media applications that is often used by Indonesians and is popular among Generation Z is the Instagram application. This research aims to see the influence between the use of Instagram on Generation Z's communication behavior by using a quantitative approach method, through distributing questionnaires to respondents through the Google Form feature. The population in this study were active users of the Instagram application as many as 100 respondents. The sample was drawn using the Slovin formula. The results showed that there is a significant influence between the use of Instagram on the communication behavior of Generation Z in South Jakarta both as a whole (simultaneously), and individually (partially).
TRANSFORMASI POLITISI MILENIAL (STRATEGI ANGGOTA DPRD MILENIAL DALAM MERAIH DUKUNGAN POLITIK PADA PEMILIHAN ANGGOTA LEGISLATIF KOTA BAUBAU TAHUN 2019)
Authors: Gavoer Arief Budianto
Published: July 2024

Artikel ini menganalisis strategi anggota Dewan Perwakilan Rakyat Daerah (DPRD) generasi milenial dalam memperoleh dukungan pada Pemilihan Anggota Legislatif (PILEG) Kota Baubau tahun 2019. Penelitian ini menggunakan pendekatan studi kasus dengan data yang diperoleh melalui wawancara mendalam dengan anggota DPRD generasi milenial yang terlibat dalam PILEG tersebut. Hasil penelitian menunjukkan bahwa anggota DPRD generasi milenial menggunakan strategi inovatif dalam memperoleh dukungan masyarakat. Mereka memanfaatkan media sosial sebagai alat komunikasi utama untuk menyebarkan pesan kampanye mereka. Dengan keterampilan teknologi yang dimiliki, mereka menciptakan konten menarik seperti video kampanye, infografis, dan live streaming untuk menjangkau audiens yang lebih luas. Selain itu, mereka juga melakukan interaksi langsung dengan pemilih melalui pertemuan tatap muka kampanye jalanan, dan diskusi publik. Anggota DPRD generasi milenial juga berusaha membangun citra yang dekat dengan pemilih muda melalui partisipasi aktif dalam kegiatan pemuda dan advokasi isu-isu yang relevan bagi generasi muda. Strategi ini terbukti efektif dalam membangun koneksi emosional dengan pemilih, meningkatkan partisipasi pemilih muda, dan memperoleh dukungan yang signifikan dalam PILEG Kota Baubau tahun 2019. Penelitian ini memberikan wawasan tentang pentingnya adaptasi strategi kampanye untuk generasi milenial dan pemanfaatan teknologi dalam meraih dukungan politik di era modern
بررسی معیار های شایسته سالاری در پرتو شریعت اسلامی
Authors: محمدی پوهنمل زمری , ثاقب پوهنمل شهاب الدین
Published: July 2024

چکیده هدف از نگارش این مقاله بیان معیار های اساسی استخدام اشخاص در مناصب دولتی است؛ زیرا رعایت این معیار ها از یک طرف باعث تأمین عدالت شده و از طرف دیگر ختم فساد می¬گردد، از¬این رو وقتی متوجه جوامع کشورهای جهان سوم می¬شویم می¬بینیم که عدم رعایت همین معیار‌ها سبب بیکاری¬،¬ عقبمانی و فقر در این جوامع شده است؛ بنابر این، برای اهل تخصص زمینه کار موجود نبوده و افراد در نظام بدون هیچ معیاری استخدام می¬شوند گویی این مناصب را از پدر در پدر به ارث برده اند که این امر سبب به میان آمدن فاصله میان دولت و رعیت می¬گردد. از این رو ما معیارهای شرعی را در خصوص گزینش افراد در نظام به بحث ¬گرفتیم تا بیان نماییم که هدف شریعت اسلامی این است که اصلاح به میان آید، لذا این معیارها را بررسی نمودیم تا بروی این درد مهلک که جامعه اسلامی از آن رنج می‌برد؛ درمانی پیدا شود. ما در این مقاله بیان کردیم مأمورین دولتی در نظام استخدام می‌شوند باید مسلمان، امانت کار، متخصص دارای قوت جسمی باشند بنابر این کسانیکه دارای چنین اوصاف نباشند از نظر شرع گمارش آن‌ها مشروع نمی‌باشد. همچنین در این جا به ذکر نمونه های رعایت شایسته سالاری در عهد نبوی و خلافت راشده اشاره شده و بحث خویش را با نتیجه گیری و ذکر مراجع به پایان رسانیدیم روش تحقیق در این مقاله روش توصیفی و تحلیلی می¬باشد. کلمات کلیدی: امانت، تقرر، شایسته سالاری، ماموریت، نظام. Abstract: The aim of writing this article is to establish the constitutional criteria for the employment of persons at the governance; of course, compliance with this criterion on the one hand ensures justice and on the other hand, ends deterioration! Therefore when it comes to third-country societies we can see that to be deferred and comply with these standards causes joblessness, corruption, and poverty. Because there is no job field for professional and expert persons, people are hired without any system and ideal criteria, as if they inherited these positions, which causes a gap between the government and the people. Therefore, we discuss the Shari'a standards regarding recruiting in the system to express that the subject of Islamic Sharia is to bring about reformism. Therefore, we examined these criteria to overcome this deadly pain that the Islamic society is suffering from; Find a cure. We have stated here that government officials are employed in the system, they must be Muslim, depositary, and involve experts with physical strength, therefore, those who do not have this character are not legitimate from the point of view of Sharia, similarity, and we mention an example. Competency has been mentioned in the prophetic covenant and caliphate, and we have summed up our argument with conclusions, suggestions, and bibliography. The research method used in this article is descriptive and analytical. Keywords: Trust, Recruitment, Meritocracy, Job, System.
Uso de níveis de ureia em silagens de sorgo submetidas à exposição aeróbica
Authors: Marinho Julian Vanessa Nascimento , Dib Karollayne da Silva , Cunha Raquel Nascimento da , Lima Ronaldo Francisco de , Souza Ronan Magalhães de , Guimarães Andrea Krystina Vinente
Published: July 2024

Objetivou-se avaliar os efeitos de níveis de ureia e exposição aeróbica sobre a composição químico-bromatológica e estabilidade aeróbia em silagens de sorgo. A cultivar do sorgo utilizada foi Santa Elisa. O delineamento experimental utilizado foi o inteiramente casuali-zado, em esquema fatorial 5 x 2, composto por cinco níveis de ureia - 0; 0,5; 1,5; 2,5; e 3,5% com base na matéria seca (MS) - e dois tempos de exposição aeróbia (0 e 72h). Foram quatro repetições por tratamen-tos, totalizando 20 silos experimentais. Para os dados referentes às perdas de gases, perdas de MS e recuperação de MS, utilizou-se apenas o tempo de 0h. Após 100 dias, os silos foram abertos e realizadas a avaliação sensorial, análises químico-bromatológica e avaliação da estabilidade aeróbia. Os dados da estabilidade aeróbia foram submetidos à análise descritiva e as médias da composição química–bromatológica foram analisadas por meio do programa computacional SAS. As médias foram submetidas à análise de variância através do PROC GLM, onde foram testados os contrastes linear e quadrático, considerando a proba-bilidade de 5% (p < 0,05) como nível de significância estatística. A adição de ureia promoveu efeito (p < 0,05) para MS e matéria mineral em relação ao tempo de 72h de exposição anaeróbia. A proteína bruta (PB) apresentou efeito crescente (p < 0,05) no tempo de 0h e aumento nos teores, chegando a 7,5%. Para extrato etéreo, compostos nitrogenados insolúveis no detergente ácido e proteína insolúvel no detergente ácido, a adição de ureia promoveu efeito em ambos os tempos. A estabilidade aeróbia permaneceu estável até 108h de exposição aeróbia. Como conclusão, a adição de ureia promoveu modificações na composição químico-bromatológica do material, aumentou os teores de PB e afetou as frações fibrosas, não inibindo o crescimento de microrganismos indesejáveis em silagens expostas por 72h. Os teores de MS e de nitrogênio amoniacal nas silagens expostas por 72 h ficaram fora do valor desejado para uma silagem de boa qualidade, sendo inviável ao consumo pelo animal. Em relação à estabilidade, as silagens permaneceram até 84 horas após a abertura dos silos, tendo que ser descartadas após esse tempo. Diante dos resultados obtidos, não recomenda-se o uso da ureia em silagens de sorgo que precisem ficar expostas por um médio e longo período de tempo.
Conservative treatment of lumbar vertebral injury in green iguana
Authors: Seixas Pablo de Almeida Souza , Ferreira Paulo Roberto Bahiano , Andrade Marcio de Almeida Couto , Araújo Felipe Purcell de , Souza Vinicius Ricardo Cuña de , Ventura Mateus Freitas , Gondim Leane Souza Queiroz , Pessoa Lourdes Marina Bezerra
Published: July 2024

In reptile practice, alterations in the spine are usually re-lated to osteometabolic disease. Although polytrauma in reptiles is common, publications about traumatic vertebral lesions in saurians and their clinical resolution are scarce. The objective of this case report was to describe the conservative treatment used on a young, female, free-living green iguana (Iguana iguana) that was attended by the Wild and Exotic Animals Sector of the Veterinary Medicine Hospital Renato Rodemberg de Medeiros Neto (Universidade Federal da Bahia), with diagnose, through physical and radiographic exami-nation, of luxation of the spine at the lumbar level, which resulted in compression of the spinal cord. Conserva-tive therapy was performed with the use of external immobilization, pain control and anti-inflammatory, in addition to support and acupuncture sessions. In this case, the therapy used proved to be successful, with the patient being discharged after four weeks.
Analysis of municipal waste management in the Municipality of Krakow
Authors: Szyba Marta , Ostrowska Justyna
Published: July 2024

Rational waste management is an essential element in the process of transition the linear economy into a circular model. Actions taken to achieve better results in this area are important for regional and local development as well as for the implementation of sustainable development goals. The article presents the requirements and rules for the functioning of waste management in Poland. Taking into account the structure of the system, the functioning of waste management in the Municipality of Krakow was analyzed. Particular attention was paid to the problem of municipal waste segregation by the inhabitants of Krakow. As part of own research, surveys were conducted among the employees of Municipal Cleaning Company LLC (Miejskie Przedsiębiorstwo Oczyszczania w Krakowie Sp. z o.o.), which showed the scale of the deficiencies. The results of the research were helpful in proposing actions to increase the effectiveness of the waste segregation system in Krakow.
Human receptive endometrial organoid for deciphering the implantation window
Authors: Zhang Yu , Zhao Rusong , Yang Chaoyan , Song Jinzhu , Liu Peishu , Li Yan , Liu Boyang , Li Tao , Yin Changjian , Lu Minghui , Hou Zhenzhen , Zhang Chuanxin , Chen Zi-Jiang , Wu Keliang , Zhao Han
Published: July 2024

Human endometrial receptivity is a critical determinant of pregnancy success; however, in vivo studies of its features and regulation are particularly challenging due to ethical restriction. Recently, the development of human endometrial organoids has provided a powerful model to examine this intricate biological process. In this study, we established a specialized human window-of-implantation (WOI) endometrial organoid system that mimics the in vivo receptive endometrium. The endometrial organoid recapitulates not only the structural attributes of glands and stroma, but also the in vivo endometrium’s cell types at the single-cell level. Furthermore, the WOI endometrial organoid exhibits the features of in vivo implantation endometrium such as hormone responsiveness, secretory functions, and distinctive implantation window hallmarks, including decidualization, extracellular matrix (ECM) remodeling, pinopode formation, cilia generation and metabolism. This in vitro model also effectively demonstrates the process of proliferation-secretory transformation, ciliary epithelium differentiation, and epithelial-mesenchymal transition (EMT). As such, WOI organoids hold great promise as an alternative platform to unravel the intricate mechanisms governing endometrial receptivity regulation, maternal-fetal interactions, and associated diseases, ultimately driving impactful advancements in the field.
The transcription factor Sfp1 imprints specific classes of mRNAs and links their synthesis to cytoplasmic decay
Authors: Kelbert M , Jordán-Pla A , de-Miguel-Jiménez L , García-Martínez J , Selitrennik M , Guterman A , Henig N , Granneman S , Pérez-Ortín JE , Chávez S , Choder M
Published: July 2024

To function effectively as an integrated system, the transcriptional and post-transcriptional machineries must communicate through mechanisms that are still poorly understood. Here we focus on Sfp1, known to regulate transcription of proliferation-related genes. We show that Sfp1 can regulate transcription either by binding to promoters, like most known transcription activators, or by binding to the transcribed regions (gene bodies), probably via RNA polymerase II (Pol II). We further studied the first mode of Sfp1 activity and found that, following promoter binding, Sfp1 binds to gene bodies and affects Pol II configuration, manifested by dissociation or conformational change of its Rpb4 subunit and increased backtracking. Surprisingly, Sfp1 binds to a subset of mRNAs co-transcriptionally and stabilizes them. The interaction between Sfp1 and its client mRNAs is controlled by their respective promoters and coincides with Sfp1’s dissociation from chromatin. Intriguingly, Sfp1 dissociation from the chromatin correlates with the extent of the backtracked Pol II. We propose that, following promoter recruitment, Sfp1 accompanies Pol II and regulates backtracking. The backtracked Pol II is more compatible with Sfp1’s relocation to the nascent transcripts, whereupon Sfp1 accompanies these mRNAs to the cytoplasm and regulates their stability. Thus, Sfp1’s co-transcriptional binding imprints the mRNA fate, serving as a paradigm for the cross-talk between the synthesis and decay of specific mRNAs. The interplay between Sfp1’s two modes of transcription regulation remains to be examined.
Caenorhabditis elegans SEL-5/AAK1 regulates cell migration and cell outgrowth independently of its kinase activity
Authors: Knop Filip , Zounarová Apolena , Šabata Vojtěch , Middelkoop Teije Corneel , Macůrková Marie
Published: July 2024

During Caenorhabditis elegans development, multiple cells migrate long distances or extend processes to reach their final position and/or attain proper shape. The Wnt signalling pathway stands out as one of the major coordinators of cell migration or cell outgrowth along the anterior-posterior body axis. The outcome of Wnt signalling is fine-tuned by various mechanisms including endocytosis. In this study, we show that SEL-5, the C. elegans orthologue of mammalian AP2-associated kinase AAK1, acts together with the retromer complex as a positive regulator of EGL-20/Wnt signalling during the migration of QL neuroblast daughter cells. At the same time, SEL-5 in cooperation with the retromer complex is also required during excretory canal cell outgrowth. Importantly, SEL-5 kinase activity is not required for its role in neuronal migration or excretory cell outgrowth, and neither of these processes is dependent on DPY-23/AP2M1 phosphorylation. We further establish that the Wnt proteins CWN-1 and CWN-2, together with the Frizzled receptor CFZ-2, positively regulate excretory cell outgrowth, while LIN-44/Wnt and LIN-17/Frizzled together generate a stop signal inhibiting its extension.
The influence of the secondary electrons induced by energetic electrons impacting the Cassini Langmuir probe at Saturn
Authors: Garnier P. , Holmberg M. K. G. , Wahlund J.‐E. , Lewis G. R. , Grimald S. Rochel , Thomsen M. F. , Gurnett D. A. , Coates A. J. , Crary F. J. , Dandouras I.
Published: October 2013

The Cassini Langmuir Probe (LP) onboard the Radio and Plasma Wave Science experiment has provided much information about the Saturnian cold plasma environment since the Saturn Orbit Insertion in 2004. A recent analysis revealed that the LP is also sensitive to the energetic electrons (250–450 eV) for negative potentials. These electrons impact the surface of the probe and generate a current of secondary electrons, inducing an energetic contribution to the DC level of the current‐voltage (I‐V) curve measured by the LP. In this paper, we further investigated this influence of the energetic electrons and (1) showed how the secondary electrons impact not only the DC level but also the slope of the (I‐V) curve with unexpected positive values of the slope, (2) explained how the slope of the (I‐V) curve can be used to identify where the influence of the energetic electrons is strong, (3) showed that this influence may be interpreted in terms of the critical and anticritical temperatures concept detailed by Lai and Tautz (2008), thus providing the first observational evidence for the existence of the anticritical temperature, (4) derived estimations of the maximum secondary yield value for the LP surface without using laboratory measurements, and (5) showed how to model the energetic contributions to the DC level and slope of the (I‐V) curve via several methods (empirically and theoretically). This work will allow, for the whole Cassini mission, to clean the measurements influenced by such electrons. Furthermore, the understanding of this influence may be used for other missions using Langmuir probes, such as the future missions Jupiter Icy Moons Explorer at Jupiter, BepiColombo at Mercury, Rosetta at the comet Churyumov‐Gerasimenko, and even the probes onboard spacecrafts in the Earth magnetosphere.
X‐linked sideroblastic anemia due to ALAS2 intron 1 enhancer element GATA‐binding site mutations
Authors: Campagna Dean R. , de Bie Charlotte I. , Schmitz‐Abe Klaus , Sweeney Marion , Sendamarai Anoop K. , Schmidt Paul J. , Heeney Matthew M. , Yntema Helger G. , Kannengiesser Caroline , Grandchamp Bernard , Niemeyer Charlotte M. , Knoers Nine V.A.M. , Swart Sonia , Marron Gordon , van Wijk Richard , Raymakers Reinier A. , May Alison , Markianos Kyriacos , Bottomley Sylvia S. , Swinkels Dorine W. , Fleming Mark D.
Published: October 2013

X‐linked sideroblastic anemia (XLSA) is the most common form of congenital sideroblastic anemia. In affected males, it is uniformly associated with partial loss‐of‐function missense mutations in the erythroid‐specific heme biosynthesis protein 5‐aminolevulinate synthase 2 (ALAS2). Here, we report five families with XLSA owing to mutations in a GATA transcription factor binding site located in a transcriptional enhancer element in intron 1 of the ALAS2 gene. As such, this study defines a new class of mutations that should be evaluated in patients undergoing genetic testing for a suspected diagnosis of XLSA. Am. J. Hematol. 89:315–319, 2014. © 2013 Wiley Periodicals, Inc.
Clinically Significant Renal Involvement in Primary Sjögren's Syndrome: Clinical Presentation and Outcome
Authors: Goules Andreas V. , Tatouli Ioanna P. , Moutsopoulos Haralampos M. , Tzioufas Athanasios G.
Published: October 2013

Objective

To estimate the prevalence and investigate the clinical features and the outcome of clinically significant renal involvement in a large cohort of patients with primary Sjögren's syndrome (SS).

Methods

Among 715 patients who met the American–European Consensus Group criteria for primary SS, those with clinically significant renal involvement were identified and their clinical and immunologic features were recorded. The prognosis in patients with primary SS with renal involvement was assessed by the clinical appearance of any of the following major outcomes: death, hemodialysis, chronic renal failure (CRF), and lymphoma. Kaplan‐Meier analysis was applied to compare death rates between patients without and those with renal involvement.

Results

Thirty‐five patients with primary SS (4.9%) had clinically significant renal involvement, representing a total followup time after renal diagnosis of 252.2 person‐years. Thirteen patients (37.1%) had interstitial nephritis alone, 17 patients (48.6%) had glomerulonephritis (GN) alone, and 5 patients (14.3%) had both entities. Nine patients died (25.7%), 11 developed CRF (including 4 requiring chronic hemodialysis) (31.4%), and 9 developed lymphoma (25.7%). The overall 5‐year survival rate was 85%. Kaplan‐Meier analysis showed statistically significant reduced survival for patients with primary SS with renal involvement compared to those without renal involvement (P < 0.0001 by log rank test), with GN patients displaying increased mortality. Eight of 9 reported deaths (89%) and 8 of 9 lymphomas (89%) were observed among patients with GN.

Conclusion

The long‐term prognosis varies for patients with primary SS who have clinically significant renal involvement. Patients with interstitial nephritis display a favorable prognosis, while patients with GN are at high risk of developing lymphoma and have poor survival.
Recovery of cell‐free mRNA and microRNA from human semen based on their physical nature
Authors: Wang Liping , Lv Jinming , Guo Cuicui , Li Honggang , Xiong Chengliang
Published: October 2013

Cell‐free seminal mRNA (cfs‐mRNA) and microRNA (cfs‐miRNA) have been found in human ejaculate and reported as promising noninvasive biomarkers for disorders of male reproductive organs and forensic identification. However, seminal plasma is particularly challenging for RNA extraction due to its complicated composition and high content of protein, DNA, and polysaccharide. Here, we report a novel, simple, and reliable method for the isolation of cfs‐mRNA and cfs‐miRNA from human semen based on our previous findings of their physical nature. Seminal microvesicles (0.1–0.5 µm in diameter), which contain the majority of cfs‐mRNA, were enriched by a microfilter. Protein complexes, which most cfs‐miRNA is bound with, were enriched by an ultrafilter. Harvesting the complexes or microvesicles, in which RNAs exist, avoided the influence of other components in human semen, thus favoring RNA isolation and purification. This new method can efficiently isolate cfs‐mRNA and cfs‐miRNA separately based on their physical nature, with high RNA purity, and low DNA contamination. It may also be applied or modified to isolate cell‐free RNAs in other fluids.
CHO cell culture longevity and recombinant protein yield are enhanced by depletion of miR‐7 activity via sponge decoy vectors
Authors: Sanchez Noelia , Kelly Paul , Gallagher Clair , Lao Nga T. , Clarke Colin , Clynes Martin , Barron Niall
Published: October 2013

Improving the efficiency of recombinant protein production by CHO cells is highly desirable as more complex proteins (MAbs, fusion proteins, blood/clotting factors, etc.) go into development and come onto the market. Previous reports have shown that microRNA (miRNA)‐7 overexpression arrests the growth of CHO cells and that its depletion increases the proliferation of various cell types. In this study we generated stable CHO clones that overexpressed a miR‐7‐specific decoy transcript (sponge) downstream of a green fluorescent protein reporter gene. The miR‐7 sponge efficiently diverted miR‐7 away from its endogenous targets as exemplified by the increased expression of CDC7. Although the sponge effectively sequestered miR‐7, it also appeared to protect the bound miRNA sequence from degradation in the cell, as exemplified by the apparent increase in mature miR‐7 levels without any change in primary transcription. Phenotypically, CHO clones with sequestered miR‐7 displayed improved maximum cell density (40%), significantly improved viability and an almost two‐fold increase in yield of secreted protein in a fed‐batch culture. These findings demonstrate that miRNA sponge transcripts could potentially be used in cell line development projects to generate producer clones that grow to higher densities and last longer in the bioreactor – thereby improving product yield.
Metabolic profiling of insect cell lines: Unveiling cell line determinants behind system's productivity
Authors: Monteiro Francisca , Bernal Vicente , Saelens Xavier , Lozano Ana B. , Bernal Cristina , Sevilla Angel , Carrondo Manuel J.T. , Alves Paula M.
Published: October 2013

Baculovirus infection boosts the host biosynthetic activity towards the production of viral components and the recombinant protein of interest, hyper‐productive phenotypes being the result of a successful adaptation of the cellular network to that scenario. Spodoptera frugiperda derived Sf9 and Trichoplusia ni derived High Five cell lines have a major track record for the production of recombinant proteins, with High Five cells presenting higher productivities. A metabolic profiling of the two insect cell lines was pursued to underpin specific cellular traits behind productive phenotypes. Multivariate analysis identified cell‐line dependent metabolic signatures linked to productivity. Pathway analysis highlighted cellular pathways of paramount importance in supporting infection and protein production. Moreover, better producer phenotypes proved to be correlated with the capacity of cells to shift their metabolism in favor of energy‐generating pathways to fuel biosynthesis, a scenario observed in the High Five cell line. Metabolomic profiling allowed us to identify metabolic pathways involved in infection and recombinant protein production, which can be selected as targets for further improvement of the system. Biotechnol. Bioeng. 2014;111: 816–828. © 2013 Wiley Periodicals, Inc.
In situ mixed donor synthesis of ampicillin with ethylene glycol co‐solvent
Authors: Deaguero Andria L. , Bommarius Andreas S.
Published: October 2013

Ethylene glycol has the ability to act as a reactant when employed as a co‐solvent for the enzymatic synthesis of ampicillin from (R)‐phenylglycine methyl ester and 6‐aminopenicillanic acid. The side reaction positively affects yield because its product, (R)‐phenylglycine hydroxyethyl ester, is an intermediate for ampicillin synthesis. Biotechnol. Biotechnol. Bioeng. 2014;111: 1054–1058. © 2013 Wiley Periodicals, Inc.
Fed‐batch synthesis of galacto‐oligosaccharides with Aspergillus oryzae β‐galactosidase using optimal control strategy
Authors: Vera Carlos , Guerrero Cecilia , Illanes Andrés , Conejeros Raúl
Published: October 2013

Fed‐batch synthesis of galacto‐oligosaccharides (GOS) from lactose with β‐galactosidase from Aspergillus oryzae was evaluated experimentally and reaction yield was maximized via optimal control technique. The optimal lactose and enzyme feed flow rate profiles were determined using a model for GOS synthesis previously reported by the authors. Experimentally it was found that fed‐batch synthesis allowed an increase on the maximum total GOS concentration from 115 (batch synthesis) to 218 g L−1 as consequence of the increase in total sugars concentration from 40 to 58% w/w. Such high concentration of total sugars was not attainable in batch operation because of the low solubility of lactose at the reaction temperature (40°C). Simulations predicted a GOS yield of 32.5 g g−1 in fed‐batch synthesis under optimal conditions, while experimentally the same yield as in batch synthesis was obtained (28 g g−1). Besides, an enrichment of total oligosaccharides in GOS with a high polymerization degree (GOS‐5 and GOS‐6) was observed in the fed‐batch synthesis. Experimental profiles for all sugars were similar to the ones predicted by simulation, which supports the use of this methodology for the optimization of GOS synthesis. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 30:59–67, 2014
SicSim: A simulator of the educational SIC/XE computer for a system‐software course
Authors: Mihelič J. , Dobravec T.
Published: October 2013

A modern computer system provides its support via system software that consists of applications such as an assembler, a linker, a loader and virtual machines. It is of prime importance to give students that are learning system‐software concepts a solid base of knowledge without any unnecessary details. To make the subject easy to understand we designed a simulator for a hypothetical computer that is already used in several courses on system software. In the paper, we describe the simulator's behavior as well as its design and implementation. Additionally, we present three case studies of using a simulator in teaching and describe our experience of its use in a course on system software. From the experience of using the simulator in a pedagogical process we conclude that it decreases the time invested by the students to comprehend the topic, and at the same time it enables in depth understanding. © 2013 Wiley Periodicals, Inc. Comput Appl Eng Educ 23:137–146, 2015; View this article online at wileyonlinelibrary.com/journal/cae; DOI 10.1002/cae.21585
Sorption and pervaporation results of clinoptilolite filled poly(vinylalcohol) membrane prepared for dehydration of aqueous organic mixtures
Authors: Salt Yavuz , Arçevik Ersel , Ekinci Buket
Published: October 2013

Clinoptilolite filled poly(vinylalcohol) membranes containing different weight percentages of zeolite were prepared by solution casting method, and the prepared membranes were characterised by scanning electron microscopy, thermogravimetric analysis and differential scanning calorimeter. Sorption and permeation experiments were carried out at 30, 40 and 50°C in pure water, ethanol (EtOH) and ethylacetate. In addition, the pervaporation separation of EtOH–water mixtures was conducted for 20–80 wt% water containing EtOH–water binary mixtures at 30, 40 and 50°C. It is observed that permeation rates increased and selectivity decreased with the increasing of temperature and the water content in the feed.