bluuebunny/crossref_metadata_2025 huggingface dataset:
Ultrasonic Extraction Process of Polysaccharides from Dendrobium nobile Lindl.: Optimization, Physicochemical Properties and Anti-Inflammatory Activity
Authors: Chen Hang , Shi Xueqin , Zhang Lin , Yao Li , Cen Lanyan , Li Lian , Lv Yiyi , Wei Chaoyang
Published: September 2022

To optimize the ultrasonic extraction process of polysaccharides from Dendrobium nobile Lindl. (DNP), the extraction method was conducted through a single-factor test and the response-surface methodology (RSM). With the optimal extraction process (liquid–solid ratio of 40 mL/g, ultrasonic time of 30 min, and ultrasonic power of 400 W), the maximum extraction yield was 5.16 ± 0.41%. DNP1 and DNP2 were then fractionated via DEAE-QFF and Sephacryl S-300 HR chromatography. The molecular weight (Mw) of DNP1 was identified as 67.72 kDa, composed of Man (75.86 ± 0.05%) and Glc (24.14 ± 0.05%), and the Mw of DNP2 was 37.45 kDa, composed of Man (72.32 ± 0.03%) and Glc (27.68 ± 0.03%). Anti-inflammatory assays results showed that as DNPs were 200 μg/mL, and the contents of NO, TNF-α, IL-1β, IL-6 and IL-10 in LPS-induced RAW 264.7 cells were about 13.39% and 13.39%, 43.88% and 43.51%, 17.80% and 15.37%, 13.84% and 20.66%, and 938.85% and 907.77% of those in control group, respectively. It was indicated that DNP1 and DNP2 inhibited the inflammatory response of RAW 264.7 cells induced by LPS via suppressing the level of NO and pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) and promoting the secretion of anti-inflammatory cytokine (IL-10). Therefore, DNP1 and DNP2 have potential applications in the treatment of inflammatory injury.
Stronger Together. Poly(Styrene) Gels Reinforced by Soft Gellan Gum
Authors: Getya Dariya , Gitsov Ivan
Published: September 2022

This study targets the synthesis of novel semi-interpenetrating networks and amphiphilic conetworks, where hydrophilic soft matter (Gellan Gum, GG) was combined with hydrophobic rigid poly(styrene), PSt. To achieve that, GG was chemically modified with 4-vinyl benzyl chloride to form a reactive macromonomer with multiple double bonds. These double bonds were used in a copolymerization with styrene to initially form semi-interpenetrating networks (SIPNs) where linear PSt was intertwined within the GG-PSt conetwork. The interpenetrating linear PSt and unreacted styrene were extracted over 3 consecutive days with yields 18–24%. After the extraction, the resulting conetworks (yields 76–82%) were able to swell both in organic and aqueous media. Thermo-mechanical tests (thermal gravimetric analysis, differential scanning calorimetry, and dynamic mechanical analysis) and rheology indicated that both SIPNs and conteworks had, in most cases, improved thermal and mechanical properties compared to pure poly(styrene) and pure GG gels. This crosslinking strategy proved that the reactive combination of a synthetic polymer and a bio-derived constituent would result in the formation of more sustainable materials with improved thermo-mechanical properties. The binding ability of the amphiphilic conetworks towards several organic dyes was high, showing that they could be used as potential materials in environmental clean-up.
Eating Patterns in Children with Autism Spectrum Disorder
Authors: Ahumada Danay , Guzmán Barbara , Rebolledo Soledad , Opazo Karol , Marileo Luis , Parra-Soto Solange , Viscardi Sharon
Published: September 2022

The purpose of this research was to analyze the eating patterns of preschool- and school-aged children with ASD, as provided by their families, in the La Araucanía Region of Chile. It involved a cross-sectional study with 72 families with children diagnosed with ASD aged between 2 and 12 years old. Food selectivity, appetite, body mass index (BMI) and frequency of food consumption were studied. The research determined that 97.67% present food selectivity, corresponding to alterations in the frequency of consumption of specific food groups. Moreover, 93.06%, 90.28%, 80.56% and 62.50% of children in the study do not meet the daily recommendations for fruit, fish, water and vegetable consumption, respectively. Therefore, it is important for these findings to be considered when designing and carrying out educational interventions regarding food in families with children with ASD for greater assertiveness and effectiveness in improving health.
Postharvest Exogenous Melatonin Treatment of Table Grape Berry Enhances Quality and Maintains Bioactive Compounds during Refrigerated Storage
Authors: Nasser Mohamed A. , El-Mogy Mohamed M. , Samaan Mina S. F. , Hassan Karim M. , El-Sayed Salwa M. , Alsubeie Moodi Saham , Darwish Doaa Bahaa Eldin , Mahmoud Samy F. , Al-Harbi Nadi Awad , Al-Qahtani Salem Mesfir , Alzuaibr Fahad M. , Abd El-Gawad Hany G.
Published: September 2022

Table grape berries are classified as a perishable crop that deteriorates quickly after harvest. The application of melatonin after harvest was found to be effective for retarding senescence and slowing ripening. In the current study, we tested the influence of two melatonin concentrations (50 and 100 µmol) as a postharvest application on quality, bioactive compounds, and enzyme activities of grape berries cv “Crimson” stored at 0 ± 1 °C and 90% relative humidity (RH) for 35 days. Our results indicated that melatonin application extends the shelf-life of berries by reducing weight loss and maintaining total soluble solids (TSS), titratable acidity (TA), berry adherence strength, and firmness. Melatonin treatment also reduced pectin methyl esterase (PME) and polygalactouranase (PG) enzyme activities compared to the control. Moreover, O2•− and H2O2 rates in berries were reduced by high melatonin concentration. Moreover, peroxidase (POD) and catalase (CAT) enzyme activities were increased by melatonin application. Our findings suggested using melatonin postharvest to increase shelf life and maintain quality attributes during refrigerated storage, which could be advantageous on a large scale.
Checklist of Odonata of Sidi Bel Abbes, Northwest Algeria
Authors: El Bouhissi Mayssara , Chaib Sara , Houhamdi Moussa , Khelifa Rassim
Published: September 2022

The odonates of Algeria have been studied for more than a century and a half, but the Northwestern part of the country has historically received little attention. A recent study in central North Algeria reported a species new to the country, suggesting that new investigations in unexplored areas are necessary to fully understand the local odonatofauna. We studied assemblages of odonates in 23 sites in Sidi Bel Abbes (Northwest Algeria) by bimonthly recording adults across a 200 m-transect from May to August of 2019–2022. Our sampling yielded 29 species (13 damselflies and 16 dragonflies) belonging to 19 genera and seven families. We recorded a new locality for the regionally endangered Coenagrion mercuriale, expanding the western range limit of the species in Algeria. We documented the occurrence of Selysiothemis nigra, a species that has recently become more frequent in North Africa. Ischnura pumilio and Onychogomphus forcipatus unguiculatus, which are relatively rare in the region, were also recorded. The arid-dwelling Trithemis kirbyi and the Mediterranean endemic Orthetrum nitidinerve were quite common in the study area. This study fills an important gap of knowledge in our understanding of odonate geographic distribution in North Africa.
Experimental Investigation on Red Mud from the Bayer Process for Cemented Paste Backfill
Authors: Bian Jiwei , Li Shuai , Zhang Qinli
Published: September 2022

Red mud is a by-product of alumina production, and its disposal can have severe environmental consequences. This study experimentally investigates the feasibility of using red mud from the Bayer process for cemented paste backfill (CPB). Different binders and activators were used to improve the mechanical properties, water resistance, and environmental behaviors of red mud-based CPB. In addition, water immersion tests were introduced, for the first time, to evaluate the water resistance of CPB. Furthermore, the environmental behaviors of red mud-based CPB were investigated by conducting leaching experiments. The results showed that the red-mud specimens had an unconfined compressive strength (UCS) of less than 0.2 MPa and disintegrated after being immersed in water. Different binders significantly improved the mechanical properties of red mud-based CPB. In addition, the specimens with different binders showed excellent water resistance, and the softening coefficient of CPB with different binders could exceed 0.7 after being cured for 28 days. The binders exhibited a substantial inhibitory effect on the leaching of hazardous substances in red mud under the solidification and stabilization effects. The leaching concentration of hexavalent chromium, selenium, fluoride, arsenic, lead, and vanadium was reduced by more than 70%. Therefore, this study provides an effective method for the environmental-friendly and large-scale utilization of red mud from the Bayer process.
Alcohol Consumption and Adiposity: A Longitudinal Analysis of 45,399 UK Biobank Participants
Authors: Inan-Eroglu Elif , Huang Bo-Huei , Hamer Mark , Britton Annie , Stamatakis Emmanuel
Published: September 2022

The evidence on the association between alcohol consumption and adiposity is inconsistent and fragmented. We investigated the longitudinal association between alcohol consumption pattern and four different adiposity markers with repeated measures of adiposity and obesity incidence. We categorized current drinkers based on the sex-specific quartiles of their weekly alcohol consumption and the UK alcohol drinking guidelines. We used multivariable adjusted generalised linear models. With the exception of a direct association between alcohol volume and body fat percentage (BF%) in women (B = 0.42%; 95%CI: 0.04, 0.80% for women in the top quartile), we found no associations between alcohol consumption and adiposity markers for either sex. Red wine and champagne/white wine consumption were inversely associated with waist circumference (WC) for both sexes (B = −0.58 cm, 95%CI: −0.77, −0.38 cm and B= −0.49 cm, 95%CI: −0.68, −0.29 cm, respectively, for women; B = −0.28 cm, 95%CI: −0.47, −0.08 cm and B = −0.23 cm, 95%CI: −0.42, −0.04 cm, respectively, for men). Female and male spirit drinkers had higher WC than non-spirit drinkers. Alcohol consumption was associated with a lower risk of obesity incidence in women (OR:0.60, 95%CI:0.45, 0.80 for the 2nd quartile, OR:0.53, 95%CI: 0.40, 0.70 for the 3rd quartile and OR:0.61, 95%CI:0.46, 0.80 for the 4th quartile). We found limited evidence of longitudinal associations between alcohol intake and adiposity. The few statistically significant associations we observed are unlikely to be of clinical importance.
Effects of Overt and Relational Bullying on Adolescents’ Subjective Well-Being: The Mediating Mechanisms of Social Capital and Psychological Capital
Authors: Hu Wenyan , Cheng Yuhang , Du Ruoyu
Published: September 2022

Based on the social and psychological capital framework, this study aimed to investigate the direct effect of bullying on adolescents’ subjective well-being and to reveal the potential psychosocial mechanisms in this relationship. Through the multi-stage cluster random sampling procedure, a cross-sectional survey was conducted among 728 adolescents from Hebei Province in China. Structural equation modeling was adopted for data analysis. After controlling for sociodemographic variables, the results indicated that only relational bullying had a significant negative effect on adolescents’ subjective well-being. Moreover, social capital and psychological capital mediated the relationship between relational bullying and adolescents’ subjective well-being. This study expands our understanding of the influencing mechanisms from bullying victimization to subjective well-being and also provides practical implications for future social policy development and relevant interventions.
Home- and Community-Based Interventions for Physical Activity and Early Child Development: A Systematic Review of Effective Strategies
Authors: Moss Samantha , Gu Xiangli
Published: September 2022

This systematic review examined the effects of home/family and community-based interventions on physical activity (PA) and developmental outcomes in early childhood. A search strategy was employed using four electronic databases (Academic Search Complete, CINAHL Complete, MEDLINE, and SPORTDiscus). Interventions investigating weight status (i.e., BMI), physical activity, sedentary behavior, and/or motor proficiency that took place in home, family, or community settings were assessed. Studies were eligible if they were peer-reviewed, available in English, published between 2011 and 2021, and if samples consisted of healthy young children (2–5 years old). There were 24 studies retained (8351 participants) spanning from the United States (n = 12), Australia (n = 3), Canada (n = 2), Switzerland (n = 2), Finland (n = 2), Netherlands (n = 1), and other Eastern European countries (n = 2). There were 19 studies that incorporated home/family-based approaches and 14 studies that incorporated community-based approaches. Studies ranged in intervention duration from 6 weeks to 24 months. It suggests that improving PA participation in young children was especially challenging to solicit improvement (only 25% of all studies found significant improvement in PA after intervention). Distributing educational material to parents/families, consistent, direct contact with parents, and encouraging community engagement were identified as effective strategies to promote physical activity, healthy weight status, and motor skills in young children.
The Effect of Household Technology on Child Health: Evidence from China’s “Home Appliances Going to the Countryside” Policy
Authors: Shi Junhui , Wang Fang , Wang Huan
Published: September 2022

This paper examined the effects of household technology on child health using evidence from the Chinese government’s “Home Appliances Going to the Countryside” policy. A difference-in-differences approach was employed to examine 2000 to 2015 data from the China Health and Nutrition Survey data from before the policy in 2007 to after the policy was implemented. It was found that the policy-induced household technology adoption significantly increased child health, especially girls’ health. Various sensitivity tests proved this finding to be robust. The potential paths through which household technology improved child health were also examined from which it was found that parental care for children and increased nutrition were effective paths between household technology and health status. These results could guide policymakers when constructing and developing a supportive child health system in China.
Endogenous Development Models and Paths Selection of Rural Revitalization from the Perspective of Ecological Environment Advantages: A Case Study of Nanshi Village, China
Authors: Guo Xinwei , Yu Bin , Yan Meiyan , Guo Hui , Ren Junhu , Zhang Hanxia , Zhang Zonggang
Published: September 2022

This article aims to discuss how to give full play to the comparative advantages of the rural ecological environment and realize the endogenous development of rural society and economy in China. First, based on the ecological economy theory of “lucid waters and lush mountains are golden and silver mountains” (the “two mountains” theory), we integrated the theories and methods of ecology, economics, and geography disciplines to examine the transformation of “ecological advantages” into “economic development” from a comprehensive perspective. Second, based on the matching relationship between the division of major function zones and the classification of ecological services, we creatively constructed a theoretical framework for the endogenous development of rural areas. Third, accounting indicators and methods for rural ecological products’ biophysical quantity and monetary value are established. Finally, we conducted an empirical study of Nanshi Village in central China as a case. The results showed that: The benefits provided by ecosystems to the development of human society would be underestimated if it is measured only by the provisioning services; the per capita Gross Ecosystem Product (GEP) of the case area was three times the per capita disposable income of rural permanent residents in the same period. Taking advantage of the rural ecological environment to promote the actual transformation of the potential value of ecological products is the feasible path for rural revitalization. One of the implications of this study is that it links the rural ecological and environmental advantages with social and economic development from the perspective of ecological economics and provides decision-making support for this case and other similar rural ecological industry revitalization practices.
Sex Differences of Sarcopenia in an Elderly Asian Population: The Prevalence and Risk Factors
Authors: Hwang Jongseok , Park Soonjee
Published: September 2022

The loss of muscle mass is widespread in age-related health phenomena in the elderly population. This study examined the prevalence of sarcopenia in a community-dwelling elderly population according to gender. The study also identified gender-specific risk factors in older people aged 75–84 years old. One thousand two hundred and ninety-three participants aged between 75 and 84 years from the National Health and Nutrition Examination Surveys in Korea were investigated. The prevalence of sarcopenia in males and females in the weighted-value sample was 41.2% (95%CI: 35.8–46.8) and 37.2% (32.7–41.9), respectively. Gender-specific clinical risk factors in males were height, weight, body mass index, waist circumference, skeletal muscle mass index, fasting glucose, and triglyceride levels. Height, weight, body mass index, waist circumference, skeletal muscle mass index, and total cholesterols were clinical risk factors for females. These outcomes would be crucial to primary care clinicians and health care professionals when patients require a referral for early detection and treatment. Health care professionals and clinicians can quickly identify potential sarcopenic patients by acknowledging the gender-specific prevalence and risk factors.
Striatal Neurons Partially Expressing a Dopaminergic Phenotype: Functional Significance and Regulation
Authors: Troshev Dmitry , Bannikova Alyona , Blokhin Victor , Kolacheva Anna , Pronina Tatiana , Ugrumov Michael
Published: September 2022

Since the discovery of striatal neurons expressing dopamine-synthesizing enzymes, researchers have attempted to identify their phenotype and functional significance. In this study, it was shown that in transgenic mice expressing green fluorescent protein (GFP) under the tyrosine hydroxylase (TH) gene promoter, (i) there are striatal neurons expressing only TH, only aromatic L-amino acid decarboxylase (AADC), or both enzymes of dopamine synthesis; (ii) striatal neurons expressing dopamine-synthesizing enzymes are not dopaminergic since they lack a dopamine transporter; (iii) monoenzymatic neurons expressing individual complementary dopamine-synthesizing enzymes produce this neurotransmitter in cooperation; (iv) striatal nerve fibers containing only TH, only AADC, or both enzymes project into the lateral ventricles, providing delivery pathways for L-3,4-dihydroxyphenylalanine and dopamine to the cerebrospinal fluid; and (v) striatal GFP neurons express receptor genes for various signaling molecules, i.e., classical neurotransmitters, neuropeptides, and steroids, indicating fine regulation of these neurons. Based on our data, it is assumed that the synthesis of dopamine by striatal neurons is a compensatory response to the death of nigral dopaminergic neurons in Parkinson’s disease, which opens broad prospects for the development of a fundamentally novel antiparkinsonian therapy.
Influence of Heat Treatment on Surface, Structural and Optical Properties of Nickel and Copper Phthalocyanines Thin Films
Authors: Popielarski Pawel , Mosińska Lidia , Skowronski Lukasz , Szczesny Robert , Figà Viviana , Naparty Mieczyslaw , Derkowska-Zielinska Beata
Published: September 2022

The work presents the effect of annealing on the change of polycrystalline α and β phases of copper and nickel phthalocyanines. We have found that this process has a great influence on the optical properties of the vapor-deposited layers. The performed measurements showed that for various forms of MPc, the values of the refractive index and the extinction coefficient increased, and consequently, so did the absorption coefficient. The AFM images taken showed that the values before and after heating are morphologically different. Raman measurements showed that the band at about 1526 cm−1 (B1g symmetry) has higher intensity for the α form than for the β form. The intensity of this band is related to changing the form of phthalocyanine from α to β. Our measurements have shown that by changing the annealing temperature of the layers, we change their optical properties. As a consequence, we change their optoelectronic parameters, adjusting them to the requirements of new optoelectronic devices, such as solar cells, sensors, displays and OLEDs.
Neuroprotective Function of Rasagiline and Selegiline, Inhibitors of Type B Monoamine Oxidase, and Role of Monoamine Oxidases in Synucleinopathies
Authors: Naoi Makoto , Maruyama Wakako , Shamoto-Nagai Masayo
Published: September 2022

Synucleinopathies are a group of neurodegenerative disorders caused by the accumulation of toxic species of α-synuclein. The common clinical features are chronic progressive decline of motor, cognitive, behavioral, and autonomic functions. They include Parkinson’s disease, dementia with Lewy body, and multiple system atrophy. Their etiology has not been clarified and multiple pathogenic factors include oxidative stress, mitochondrial dysfunction, impaired protein degradation systems, and neuroinflammation. Current available therapy cannot prevent progressive neurodegeneration and “disease-modifying or neuroprotective” therapy has been proposed. This paper presents the molecular mechanisms of neuroprotection by the inhibitors of type B monoamine oxidase, rasagiline and selegiline. They prevent mitochondrial apoptosis, induce anti-apoptotic Bcl-2 protein family, and pro-survival brain- and glial cell line-derived neurotrophic factors. They also prevent toxic oligomerization and aggregation of α-synuclein. Monoamine oxidase is involved in neurodegeneration and neuroprotection, independently of the catalytic activity. Type A monoamine oxidases mediates rasagiline-activated signaling pathways to induce neuroprotective genes in neuronal cells. Multi-targeting propargylamine derivatives have been developed for therapy in various neurodegenerative diseases. Preclinical studies have presented neuroprotection of rasagiline and selegiline, but beneficial effects have been scarcely presented. Strategy to improve clinical trials is discussed to achieve disease-modification in synucleinopathies.
Genome-Wide Association Study of Salt Tolerance-Related Traits during Germination and Seedling Development in an Intermedium-Spike Barley Collection
Authors: Sayed Mohammed A. , Maurer Andreas , Schmutzer Thomas , Schnurbusch Thorsten , Börner Andreas , Hansson Mats , Pillen Klaus , Youssef Helmy M.
Published: September 2022

Increased salinity is one of the major consequences of climatic change affecting global crop production. The early stages in the barley (Hordeum vulgare L.) life cycle are considered the most critical phases due to their contributions to final crop yield. Particularly, the germination and seedling development are sensitive to numerous environmental stresses, especially soil salinity. In this study, we aimed to identify SNP markers linked with germination and seedling development at 150 mM NaCl as a salinity treatment. We performed a genome-wide association study (GWAS) using a panel of 208 intermedium-spike barley (H. vulgare convar. intermedium (Körn.) Mansf.) accessions and their genotype data (i.e., 10,323 SNPs) using the genome reference sequence of “Morex”. The phenotypic results showed that the 150 mM NaCl salinity treatment significantly reduced all recorded germination and seedling-related traits compared to the control treatment. Furthermore, six accessions (HOR 11747, HOR 11718, HOR 11640, HOR 11256, HOR 11275 and HOR 11291) were identified as the most salinity tolerant from the intermedium-spike barley collection. GWAS analysis indicated that a total of 38 highly significantly associated SNP markers under control and/or salinity traits were identified. Of these, two SNP markers on chromosome (chr) 1H, two on chr 3H, and one on chr 4H were significantly linked to seedling fresh and dry weight under salinity stress treatment. In addition, two SNP markers on chr 7H were also significantly associated with seedling fresh and dry weight but under control condition. Under salinity stress, one SNP marker on chr 1H, 5H and 7H were detected for more than one phenotypic trait. We found that in most of the accessions exhibiting the highest salinity tolerance, most of the salinity-related QTLs were presented. These results form the basis for detailed studies, leading to improved salt tolerance breeding programs in barley.
Molecular and Functional Characterization of a Short-Type Peptidoglycan Recognition Protein, Ct-PGRP-S1 in the Giant Triton Snail Charonia tritonis
Authors: Liu Wenguang , Liu Bing , Zhang Gege , Jia Huixia , Zhang Yang , Cen Xitong , Yao Gaoyou , He Maoxian
Published: September 2022

Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) involved in host antibacterial responses, and their functions have been characterized in most invertebrate and vertebrate animals. However, little information is available regarding the potential function of PGRPs in the giant triton snail Charonia tritonis. In this study, a short-type PGRP gene (termed Ct-PGRP-S1) was identified in C. tritonis. Ct-PGRP-S1 was predicted to contain several structural features known in PGRPs, including a typical PGRP domain (Amidase_2) and Src homology-3 (SH3) domain. The Ct-PGRP-S1 gene was constitutively expressed in all tissues examined except in proboscis, with the highest expression level observed in the liver. As a typical PRR, Ct-PGRP-S1 has an ability to degrade peptidoglycan (PGN) and was proven to have non-Zn2+-dependent amidase activity and antibacterial activity against Vibrioalginolyticus and Staphylococcus aureus. It is the first report to reveal the peptidoglycan recognition protein in C. tritonis, and these results suggest that peptidoglycan recognition protein Ct-PGRP-S1 is an important effector of C. tritonis that modulates bacterial infection resistance of V. alginolyticus and S. aureus, and this study may provide crucial basic data for the understanding of an innate immunity system of C. tritonis.
Delayed Diagnosis of Congenital Combined Pituitary Hormone Deficiency including Severe Growth Hormone Deficiency in Children with Persistent Neonatal Hypoglycemia—Case Reports and Review
Authors: Smyczyńska Joanna , Pawelak Natalia , Hilczer Maciej , Lewiński Andrzej
Published: September 2022

Apart from stimulation of human growth and cell proliferation, growth hormone (GH) has pleiotropic metabolic effects in all periods of life. Severe GH deficiency is a common component of combined pituitary hormone deficiency (CPHD). CPHD may be caused by mutations in the genes encoding transcription factors and signaling molecules involved in normal pituitary development; however, often its genetic cause remains unknown. Symptoms depend on which hormone is deficient. The first symptom of GH or adrenocorticotropic hormone (ACTH) deficiency may be persistent hypoglycemia in apparently healthy newborns, which is often neglected. Diagnosing CPHD is based on decreased concentrations of hormones secreted by the anterior pituitary and peripheral endocrine glands. Findings in magnetic resonance imaging vary widely, including anterior pituitary hypoplasia/aplasia or pituitary stalk interruption syndrome (PSIS). Delayed diagnosis and treatment can be life-threatening. GH therapy is necessary to recover hypoglycemia and to improve auxological and psychomotor development. We present two girls, diagnosed and treated in our departments, in whom the diagnosis of CPHD was delayed, despite persistent neonatal hypoglycemia; and a review of similar cases, with attention paid to progress in the genetic assessments of such patients, since the introduction of whole exome sequencing that is especially important for PSIS.
Role of Extracellular Matrix and Inflammation in Abdominal Aortic Aneurysm
Authors: Stepien Karolina L. , Bajdak-Rusinek Karolina , Fus-Kujawa Agnieszka , Kuczmik Wacław , Gawron Katarzyna
Published: September 2022

Abdominal aortic aneurysm (AAA) is one of the most dangerous cardiovascular diseases, occurring mainly in men over the age of 55 years. As it is asymptomatic, patients are diagnosed very late, usually when they suffer pain in the abdominal cavity. The late detection of AAA contributes to the high mortality rate. Many environmental, genetic, and molecular factors contribute to the development and subsequent rupture of AAA. Inflammation, apoptosis of smooth muscle cells, and degradation of the extracellular matrix in the AAA wall are believed to be the major molecular processes underlying AAA formation. Until now, no pharmacological treatment has been implemented to prevent the formation of AAA or to cure the disease. Therefore, it is important that patients are diagnosed at a very early stage of the disease. Biomarkers contribute to the assessment of the concentration level, which will help to determine the level and rate of AAA development. The potential biomarkers today include homocysteine, cathepsins, osteopontin, and osteoprotegerin. In this review, we describe the major aspects of molecular processes that take place in the aortic wall during AAA formation. In addition, biomarkers, the monitoring of which will contribute to the prompt diagnosis of AAA patients over the age of 55 years, are described.
Surfactin Mitigates a High-Fat Diet and Streptozotocin-Induced Type 2 Diabetes through Improving Pancreatic Dysfunction and Inhibiting Inflammatory Response
Authors: Chen Xiaoyu , Zhao Hongyuan , Lu Yajun , Liu Huawei , Meng Fanqiang , Lu Zhaoxin , Lu Yingjian
Published: September 2022

Surfactin from Bacillus amyloliquefaciens fmb50 was utilized to treat mice with type 2 diabetes (T2DM) induced by a high-fat diet/streptozotocin (HFD/STZ). Our group’s earlier research indicated that surfactin could lower blood glucose and mitigate liver dysfunction to further improve HFD/STZ-induced T2DM through modulating intestinal microbiota. Thus, we further investigated the effects of surfactin on the pancreas and colon in mice with T2DM to elucidate the detailed mechanism. In the present study, mice with HFD/STZ-induced T2DM had their pancreatic and colon inflammation, oxidative stress, and endoplasmic reticulum stress (ERS) reduced when given oral surfactin at a dose of 80 mg/kg body weight. According to further research, surfactin also improved glucose metabolism by activating the phosphatidylinositol kinase (PI3K)/protein kinase B (Akt) signaling pathway, further protecting islets β-cell, promoting insulin secretion, inhibiting glucagon release and mitigating pancreas dysfunction. Additionally, after surfactin treatment, the colon levels of the tight junction proteins Occludin and Claudin-1 of T2DM mice were considerably increased by 130.64% and by 36.40%, respectively. These findings revealed that surfactin not only ameliorated HFD/STZ-induced pancreas inflammation and dysfunction and preserved intestinal barrier dysfunction and gut microbiota homeostasis but also enhanced insulin sensitivity and glucose homeostasis in T2DM mice. Finally, in the further experiment, we were able to demonstrate that early surfactin intervention might delay the development of T2DM caused by HFD/STZ, according to critical biochemical parameters in serum.
Breed and Feeding System Impact the Bioactive Anti-Inflammatory Properties of Bovine Milk
Authors: Salzano Angela , Di Meo Maria Chiara , D’Onofrio Nunzia , Bifulco Giovanna , Cotticelli Alessio , Licitra Francesca , Iraci Fuintino Antonio , Cascone Giuseppe , Balestrieri Maria Luisa , Varricchio Ettore , Campanile Giuseppe
Published: September 2022

In the present study, we aimed at assessing the influence of breed and feeding system on the bovine milk profile of betaines and carnitines and milk capacity in counteracting the inflammatory endothelial cell (EC) damage induced by interleukin (IL)-6. In the first experimental design, two breeds were chosen (Holstein vs. Modicana) to investigate the biomolecule content and antioxidant capacity in milk and dairy products. In the second experimental design, two feeding systems (pasture vs. total mixed ratio) were tested only in Holstein to evaluate the possible effect on the functional profile of milk and dairy products. Finally, the bulk milk from the two experimental designs was used to evaluate the efficacy of preventing IL-6-induced endothelial inflammatory damage. Results showed that Modicana milk and whey had higher biomolecule content and antioxidant activity compared to Holstein milk (p < 0.01). Milk from Holstein fed TMR showed higher concentration of γ-butyrobetaine, δ-valerobetaine (p < 0.01), and l-carnitine (p < 0.05). Similarly, whey from Holstein fed TMR also showed higher content of δ-valerobetaine, glycine betaine, l-carnitine, and acetyl-l-carnitine (p < 0.01) compared to the Holstein fed pasture. Conversely, the antioxidant activity of milk and dairy products was not affected by the feeding system. In ECs, all milk samples reduced the IL-6-induced cytokine release, as well as the accumulation of reactive oxygen species (ROS) and the induction of cell death, with the most robust effect elicited by Modicana milk (p < 0.01). Overall, Modicana milk showed a higher content of biomolecules and antioxidant activity compared to Holstein, suggesting that the breed, more than the feeding system, can positively affect the health-promoting profile of dairy cattle milk.
Approaches to Measuring the Activity of Major Lipolytic and Lipogenic Enzymes In Vitro and Ex Vivo
Authors: Wilhelm Marek , Rossmeislová Lenka , Šiklová Michaela
Published: September 2022

Since the 1950s, one of the goals of adipose tissue research has been to determine lipolytic and lipogenic activity as the primary metabolic pathways affecting adipocyte health and size and thus representing potential therapeutic targets for the treatment of obesity and associated diseases. Nowadays, there is a relatively large number of methods to measure the activity of these pathways and involved enzymes, but their applicability to different biological samples is variable. Here, we review the characteristics of mean lipogenic and lipolytic enzymes, their inhibitors, and available methodologies for assessing their activity, and comment on the advantages and disadvantages of these methodologies and their applicability in vivo, ex vivo, and in vitro, i.e., in cells, organs and their respective extracts, with the emphasis on adipocytes and adipose tissue.
Molecular Mechanisms of Cellular Injury and Role of Toxic Heavy Metals in Chronic Kidney Disease
Authors: Mishra Manish , Nichols Larry , Dave Aditi A. , Pittman Elizabeth H , Cheek John P. , Caroland Anasalea J. V. , Lotwala Purva , Drummond James , Bridges Christy C.
Published: September 2022

Chronic kidney disease (CKD) is a progressive disease that affects millions of adults every year. Major risk factors include diabetes, hypertension, and obesity, which affect millions of adults worldwide. CKD is characterized by cellular injury followed by permanent loss of functional nephrons. As injured cells die and nephrons become sclerotic, remaining healthy nephrons attempt to compensate by undergoing various structural, molecular, and functional changes. While these changes are designed to maintain appropriate renal function, they may lead to additional cellular injury and progression of disease. As CKD progresses and filtration decreases, the ability to eliminate metabolic wastes and environmental toxicants declines. The inability to eliminate environmental toxicants such as arsenic, cadmium, and mercury may contribute to cellular injury and enhance the progression of CKD. The present review describes major molecular alterations that contribute to the pathogenesis of CKD and the effects of arsenic, cadmium, and mercury on the progression of CKD.
Isolation and Sequencing of Chromosome Arm 7RS of Rye, Secale cereale
Authors: Petereit Jakob , Tay Fernandez Cassandria , Marsh Jacob I. , Bayer Philipp E. , Thomas William J. W. , Aliyeva Aybeniz Javad , Karafiátová Miroslava , Doležel Jaroslav , Batley Jacqueline , Edwards David
Published: September 2022

Rye (Secale cereale) is a climate-resilient cereal grown extensively as grain or forage crop in Northern and Eastern Europe. In addition to being an important crop, it has been used to improve wheat through introgression of genomic regions for improved yield and disease resistance. Understanding the genomic diversity of rye will assist both the improvement of this crop and facilitate the introgression of more valuable traits into wheat. Here, we isolated and sequenced the short arm of rye chromosome 7 (7RS) from Triticale 380SD using flow cytometry and compared it to the public Lo7 rye whole genome reference assembly. We identify 2747 Lo7 genes present on the isolated chromosome arm and two clusters containing seven and sixty-five genes that are present on Triticale 380SD 7RS, but absent from Lo7 7RS. We identified 29 genes that are not assigned to chromosomal locations in the Lo7 assembly but are present on Triticale 380SD 7RS, suggesting a chromosome arm location for these genes. Our study supports the Lo7 reference assembly and provides a repertoire of genes on Triticale 7RS.
Genome-Wide Characterization and Expression Analysis of Fatty acid Desaturase Gene Family in Poplar
Authors: Wei Hui , Movahedi Ali , Xu Songzhi , Zhang Yanyan , Liu Guoyuan , Aghaei-Dargiri Soheila , Ghaderi Zefrehei Mostafa , Zhu Sheng , Yu Chunmei , Chen Yanhong , Zhong Fei , Zhang Jian
Published: September 2022

Fatty acid desaturases (FADs) modulate carbon–carbon single bonds to form carbon–carbon double bonds in acyl chains, leading to unsaturated fatty acids (UFAs) that have vital roles in plant growth and development and their response to environmental stresses. In this study, a total of 23 Populus trichocarpaFAD (PtFAD) candidates were identified from the poplar genome and clustered into seven clades, including FAB2, FAD2, FAD3/7/8, FAD5, FAD6, DSD, and SLD. The exon–intron compositions and conserved motifs of the PtFADs, clustered into the same clade, were considerably conserved. It was found that segmental duplication events are predominantly attributable to the PtFAD gene family expansion. Several hormone- and stress-responsive elements in the PtFAD promoters implied that the expression of the PtFAD members was complicatedly regulated. A gene expression pattern analysis revealed that some PtFAD mRNA levels were significantly induced by abiotic stress. An interaction proteins and gene ontology (GO) analysis indicated that the PtFADs are closely associated with the UFAs biosynthesis. In addition, the UFA contents in poplars were significantly changed under drought and salt stresses, especially the ratio of linoleic and linolenic acids. The integration of the PtFAD expression patterns and UFA contents showed that the abiotic stress-induced PtFAD3/7/8 members mediating the conversion of linoleic and linolenic acids play vital roles in response to osmotic stress. This study highlights the profiles and functions of the PtFADs and identifies some valuable genes for forest improvements.
Prediction and Ranking of Biomarkers Using multiple UniReD
Authors: Baltsavia Ismini , Theodosiou Theodosios , Papanikolaou Nikolas , Pavlopoulos Georgios A. , Amoutzias Grigorios D. , Panagopoulou Maria , Chatzaki Ekaterini , Andreakos Evangelos , Iliopoulos Ioannis
Published: September 2022

Protein–protein interactions (PPIs) are of key importance for understanding how cells and organisms function. Thus, in recent decades, many approaches have been developed for the identification and discovery of such interactions. These approaches addressed the problem of PPI identification either by an experimental point of view or by a computational one. Here, we present an updated version of UniReD, a computational prediction tool which takes advantage of biomedical literature aiming to extract documented, already published protein associations and predict undocumented ones. The usefulness of this computational tool has been previously evaluated by experimentally validating predicted interactions and by benchmarking it against public databases of experimentally validated PPIs. In its updated form, UniReD allows the user to provide a list of proteins of known implication in, e.g., a particular disease, as well as another list of proteins that are potentially associated with the proteins of the first list. UniReD then automatically analyzes both lists and ranks the proteins of the second list by their association with the proteins of the first list, thus serving as a potential biomarker discovery/validation tool.
Correction: Franz et al. Unraveling a Force-Generating Allosteric Pathway of Actomyosin Communication Associated with ADP and Pi Release. Int. J. Mol. Sci. 2021, 22, 104
Authors: Franz Peter , Ewert Wiebke , Preller Matthias , Tsiavaliaris Georgios
Published: September 2022

The author wishes to make the following correction to this paper [...]
The Identification and Expression Analysis of the Nitraria sibirica Pall. Auxin-Response Factor (ARF) Gene Family
Authors: Liu Yuxin , Zhang Jingbo , Li Xinle , Zhu Liming , Lian Ziming , Fang Hao , Lu Lu , Lu Ye , Shi Jisen , Chen Jinhui , Hao Zhaodong , Cheng Tielong
Published: September 2022

Nitraria sibirica is a shrub that can survive in extreme drought environments. The auxin-response factors (ARFs) are a class of transcription factors that are widely involved in plant growth and development, as well as in the regulation of stress resistance. However, the genome-wide identification of the ARF gene family and its responses to environmental stresses, especially drought stress, in N. sibirica has not yet been reported. Here, we identified a total of 12 ARF genes in the genome of N. sibirica, which were distributed over 10 chromosomes and divided into three clades. Intragenome synteny analysis revealed one collinear gene pair in the ARF gene family, i.e., NsARF9a and NsARF9b. Cis-acting element analysis showed that multiple hormones and stress-responsive cis-acting elements were found in the promoters of NsARFs, suggesting that NsARFs may be involved in multiple biological processes. Quantitative real-time PCR (qRT-PCR) showed that many NsARFs had tissue-specific expression patterns, with the highest expression of NsARF16 in the seedlings of N. sibirica. In addition, most of the NsARFs that were upregulated under drought were independent of endogenous ABA biosynthesis, whereas the response of NsARF5 and NsARF7a to drought was disrupted by the ABA-biosynthesis inhibitor fluridone. These studies provide a basis for further research into how NsARFs in N. sibirica respond to hormonal signaling and environmental stresses.
Later Growth Cessation and Increased Freezing Tolerance Potentially Result in Later Dormancy in Evergreen Iris Compared with Deciduous Iris
Authors: Xu Tong , Zhang Jiao , Shao Lingmei , Wang Xiaobin , Zhang Runlong , Ji Chenxi , Xia Yiping , Zhang Liangsheng , Zhang Jiaping , Li Danqing
Published: September 2022

Winter dormancy is a protective survival strategy for plants to resist harsh natural environments. In the context of global warming, the progression of dormancy has been significantly affected in perennials, which requires further research. Here, a systematic study was performed to compare the induction of dormancy in two closely related iris species with an ecodormancy-only process, the evergreen Iris japonica Thunb. and the deciduous Iris tectorum Maxim. under artificial conditions. Firstly, morphological and physiological observations were evaluated to ensure the developmental status of the two iris species. Furthermore, the expression patterns of the genes involved in key pathways related to plant winter dormancy were determined, and correlation analyses with dormancy marker genes were conducted. We found that deciduous iris entered dormancy earlier than evergreen iris under artificial dormancy induction conditions. Phytohormones and carbohydrates play roles in coordinating growth and stress responses during dormancy induction in both iris species. Moreover, dormancy-related MADS-box genes and SnRKs (Snf1-related protein kinase) might represent a bridge between carbohydrate and phytohormone interaction during iris dormancy. These findings provide a hypothetical model explaining the later dormancy in evergreen iris compared with deciduous iris under artificial dormancy induction conditions and reveal some candidate genes. The findings of this study could provide new insights into the research of dormancy in perennial plants with an ecodormancy-only process and contribute to effectively managing iris production, postharvest storage, and shipping.
Meta-Analysis Identifies BDNF and Novel Common Genes Differently Altered in Cross-Species Models of Rett Syndrome
Authors: Haase Florencia , Singh Rachna , Gloss Brian , Tam Patrick , Gold Wendy
Published: September 2022

Rett syndrome (RTT) is a rare disorder and one of the most abundant causes of intellectual disabilities in females. Single mutations in the gene coding for methyl-CpG-binding protein 2 (MeCP2) are responsible for the disorder. MeCP2 regulates gene expression as a transcriptional regulator as well as through epigenetic imprinting and chromatin condensation. Consequently, numerous biological pathways on multiple levels are influenced. However, the exact molecular pathways from genotype to phenotype are currently not fully elucidated. Treatment of RTT is purely symptomatic as no curative options for RTT have yet to reach the clinic. The paucity of this is mainly due to an incomplete understanding of the underlying pathophysiology of the disorder with no clinically useful common disease drivers, biomarkers, or therapeutic targets being identified. With the premise of identifying universal and robust disease drivers and therapeutic targets, here, we interrogated a range of RTT transcriptomic studies spanning different species, models, and MECP2 mutations. A meta-analysis using RNA sequencing data from brains of RTT mouse models, human post-mortem brain tissue, and patient-derived induced pluripotent stem cell (iPSC) neurons was performed using weighted gene correlation network analysis (WGCNA). This study identified a module of genes common to all datasets with the following ten hub genes driving the expression: ATRX, ADCY7, ADCY9, SOD1, CACNA1A, PLCG1, CCT5, RPS9, BDNF, and MECP2. Here, we discuss the potential benefits of these genes as therapeutic targets.