bluuebunny/crossref_metadata_2025 huggingface dataset:
Different Immune Responses of Hemocytes from V. parahaemolyticus-Resistant and -Susceptible Shrimp at Early Infection Stage
Authors: Du Wenran , Li Shihao , Li Fuhua
Published: April 2024

Vibrio parahaemolyticus is one of the main causative agents leading to acute hepatopancreatic necrosis disease, the severe bacterial disease that occurs during shrimp aquaculture. Hemocytes play important roles during Vibrio infection. Previously, we found that there were few differentially expressed genes (DEGs) between hemocytes from V. parahaemolyticus-resistant and -susceptible shrimp before infection. We considered that there should be different immune responses between them after a pathogen infection. Here, the transcriptome data of hemocytes from V. parahaemolyticus-resistant and -susceptible shrimp before and after a pathogen infection were compared. The results showed that there were 157 DEGs responsive to infection in V. parahaemolyticus-resistant shrimp, while 33 DEGs in V. parahaemolyticus-susceptible shrimp. DEGs in V. parahaemolyticus-resistant shrimp were mainly related to immune and glycolytic processes, while those in V. parahaemolyticus-susceptible shrimp were mainly related to metabolism, with only two DEGs in common. A further analysis of genes involved in glucose metabolism revealed that GLUT2, HK, FBP, and PCK1 were lowly expressed while PC were highly expressed in hemocytes of the V. parahaemolyticus-resistant shrimp, indicating that glucose metabolism in shrimp hemocytes was related to a V. parahaemolyticus infection. After the knockdown of PC, the expression of genes in Toll and IMD signaling pathways were down-regulated, indicating that glucose metabolism might function through regulating host immunity during V. parahaemolyticus infection. The results suggest that the immune responses between V. parahaemolyticus-resistant and -susceptible shrimp were apparently different, which probably contribute to their different V. parahaemolyticus resistance abilities.
Fundus Autofluorescence in Posterior and Panuveitis—An Under-Estimated Imaging Technique: A Review and Case Series
Authors: Mauschitz Matthias M. , Zeller Markus , Sagar Pradeep , Biswal Suchitra , Guzman Gabriela , Terheyden Jan H. , Meyer Carsten H. , Holz Frank G. , Heinz Carsten , Pleyer Uwe , Finger Robert P. , Wintergerst Maximilian W. M.
Published: April 2024

Fundus autofluorescence (FAF) is a prompt and non-invasive imaging modality helpful in detecting pathological abnormalities within the retina and the choroid. This narrative review and case series provides an overview on the current application of FAF in posterior and panuveitis. The literature was reviewed for articles on lesion characteristics on FAF of specific posterior and panuveitis entities as well as benefits and limitations of FAF for diagnosing and monitoring disease. FAF characteristics are described for non-infectious and infectious uveitis forms as well as masquerade syndromes. Dependent on the uveitis entity, FAF is of diagnostic value in detecting disease and following the clinical course. Currently available FAF modalities which differ in excitation wavelengths can provide different pathological insights depending on disease entity and activity. Further studies on the comparison of FAF modalities and their individual value for uveitis diagnosis and monitoring are warranted.
Reflections on the Origin of Coded Protein Biosynthesis
Authors: Fontecilla-Camps Juan Carlos
Published: April 2024

The principle of continuity posits that some central features of primordial biocatalytic mechanisms should still be present in the genetically dependent pathway of protein synthesis, a crucial step in the emergence of life. Key bimolecular reactions of this process are catalyzed by DNA-dependent RNA polymerases, aminoacyl-tRNA synthetases, and ribosomes. Remarkably, none of these biocatalysts contribute chemically active groups to their respective reactions. Instead, structural and functional studies have demonstrated that nucleotidic α-phosphate and β-d-ribosyl 2′ OH and 3′ OH groups can help their own catalysis, a process which, consequently, has been called “substrate-assisted”. Furthermore, upon binding, the substrates significantly lower the entropy of activation, exclude water from these catalysts’ active sites, and are readily positioned for a reaction. This binding mode has been described as an “entropy trap”. The combination of this effect with substrate-assisted catalysis results in reactions that are stereochemically and mechanistically simpler than the ones found in most modern enzymes. This observation is consistent with the way in which primordial catalysts could have operated; it may also explain why, thanks to their complementary reactivities, β-d-ribose and phosphate were naturally selected to be the central components of early coding polymers.
Modeling Radiation-Induced Epithelial Cell Injury in Murine Three-Dimensional Esophageal Organoids
Authors: Carswell Latisha , Sridharan Deepa M. , Chien Lung-Chang , Hirose Wataru , Giroux Véronique , Nakagawa Hiroshi , Pluth Janice M.
Published: April 2024

Esophageal squamous cell carcinoma (ESCC) is a deadly consequence of radiation exposure to the esophagus. ESCC arises from esophageal epithelial cells that undergo malignant transformation and features a perturbed squamous cell differentiation program. Understanding the dose- and radiation quality-dependence of the esophageal epithelium response to radiation may provide insights into the ability of radiation to promote ESCC. We have explored factors that may play a role in esophageal epithelial radiosensitivity and their potential relationship to ESCC risk. We have utilized a murine three-dimensional (3D) organoid model that recapitulates the morphology and functions of the stratified squamous epithelium of the esophagus to study persistent dose- and radiation quality-dependent changes. Interestingly, although high-linear energy transfer (LET) Fe ion exposure induced a more intense and persistent alteration of squamous differentiation and 53BP1 DNA damage foci levels as compared to Cs, the MAPK/SAPK stress pathway signaling showed similar altered levels for most phospho-proteins with both radiation qualities. In addition, the lower dose of high-LET exposure also revealed nearly the same degree of morphological changes, even though only ~36% of the cells were predicted to be hit at the lower 0.1 Gy dose, suggesting that a bystander effect may be induced. Although p38 and ERK/MAPK revealed the highest levels following high-LET exposure, the findings reveal that even a low dose (0.1 Gy) of both radiation qualities can elicit a persistent stress signaling response that may critically impact the differentiation gradient of the esophageal epithelium, providing novel insights into the pathogenesis of radiation-induced esophageal injury and early stage esophageal carcinogenesis.
Efficacy of a Soft Robotic Exoskeleton to Improve Lower Limb Motor Function in Children with Spastic Cerebral Palsy: A Single-Blinded Randomized Controlled Trial
Authors: Hui Zhichong , Qi Weihang , Zhang Yi , Wang Mingmei , Zhang Jiamei , Li Dong , Zhu Dengna
Published: April 2024

Purpose: Soft robotic exoskeletons (SREs) are portable, lightweight assistive technology with therapeutic potential for improving lower limb motor function in children with cerebral palsy. To understand the effects of long-term SRE-assisted walking training on children with spastic cerebral palsy (SCP), we designed a study aiming to elucidate the effects of SRE-assisted walking training on lower limb motor function in this population. Methods: In this randomized, single-blinded (outcome assessor) controlled trial, forty children diagnosed with SCP were randomized into the routine rehabilitation (RR) group (N = 20) and the SRE group (N = 20) for comparison. The RR group received routine rehabilitation training, and the SRE group received routine rehabilitation training combined with SRE-assisted overground walking training. Assessments (without SRE) were conducted pre- and post-intervention (8 weeks after the intervention). The primary outcome measures included the 10 m walk test (10MWT) and the 6 min walk test (6MWT). Secondary outcome measures comprised the gross motor function measure-88, pediatric balance scale modified Ashworth scale, and physiological cost index. Results: Both groups showed significant improvements (p < 0.01) across all outcome measures after the 8-week intervention. Between-group comparisons using ANCOVA revealed that the SRE group demonstrated greater improvement in walking speed from the 10MWT (+6.78 m/min, 95% CI [5.74–7.83]; p < 0.001) and walking distance during the 6MWT (+34.42 m, 95% CI [28.84–39.99]; p < 0.001). The SRE group showed greater improvement in all secondary outcome measures (p < 0.001). Conclusions: The study findings suggested that the integration of SRE-assisted overground walking training with routine rehabilitation more effectively enhances lower limb motor function in children with SCP compared to routine rehabilitation alone.
Enhanced Novel Object Recognition and Spatial Memory in Rats Selectively Bred for High Nicotine Preference
Authors: Bekci Eren , Gokmen Ramazan Can , Kanit Lutfiye , Gozen Oguz , Balkan Burcu , Koylu Ersin O. , Keser Aysegul
Published: April 2024

This study examined the influence of genetic background on cognitive performance in a selectively bred high nicotine-preferring (NP) rat line. Using the novel object recognition (NOR), novel location recognition (NLR), and Morris water maze (MWM) tests, we evaluated object memory, spatial memory, and spatial navigation in nicotine-naive NP rats compared to controls. Our results demonstrate that in the NOR test, both male and female NP rats spent more time exploring the novel object (higher discrimination index) compared to sex-matched controls. In the NLR, the discrimination index differed significantly from zero chance (no preference) in both NP males and females but not in controls, indicating enhanced spatial memory in the NP line. During MWM acquisition, the NP groups and control males took a shorter path to reach the platform compared to control females. On the probe trial, the distance traveled in the target quadrant was longer for NP males and females compared to their respective controls, suggesting enhanced spatial navigation and learning in the NP rats. The interesting preference for novel objects and locations displayed by NP rats may indicate a potential novelty-seeking phenotype in this line. These results highlight the complex interplay between genetic factors, cognitive function, and nicotine preference.
On the Dynamics of Inferential Behavior while Reading Expository and Narrative Texts
Authors: Yoo Yongseok
Published: April 2024

Inference plays a key role in reading comprehension. This study examines changes in inferential behavior while reading different genres. The inferential behavior of 28 students with reading disabilities (RDs) and 44 students without RDs was quantified while they read expository and narrative texts. First, the average rates of inference attempts and correct inferences were measured during reading. Then, the same rates were measured separately during early and late reading to see if there was a change in inferential behavior. The results show that the change in inferential behavior depends on the genre. While reading the expository text, both groups showed no significant change in their inference making. In contrast, while reading the narrative text, both groups showed higher rates of inference attempts, and only the students without RD showed a significant increase in correct inferences. The implications of these findings for the design of more engaging and effective reading programs are discussed.
The Effect of Extremely Low-Frequency Magnetic Field on Stroke Patients: A Systematic Review
Authors: Marchewka Renata , Trzmiel Tomasz , Hojan Katarzyna
Published: April 2024

Background: The aim of this study was to review the current state of scientific evidence on the effect of extremely low-frequency magnetic fields stimulation (ELF-MFs) on stroke patients. Methods: A systematic review of PubMed, ScienceDirect, PeDro and Embase databases was conducted. Only articles published in English, involving adult participants and focusing on individuals who had experienced a stroke, specifically examining the impact of ELF-MFs on post-stroke patients and had well-defined criteria for inclusion and exclusion of participants, were included. The methodological quality of the included studies was assessed using the Quality Assessment Tool for Quantitative Studies (QATQS). Results: A total of 71 studies were identified through database and reference lists’ search, from which 9 were included in the final synthesis. All included studies showed a beneficial effect of ELF-MFs on stroke patients, however seven of the included studies were carried by the same research group. Improvements were observed in domains such as oxidative stress, inflammation, ischemic lesion size, functional status, depressive symptoms and cognitive abilities. Conclusions: The available literature suggests a beneficial effect of ELF-MFs on post-stroke patients; however, the current data are too limited to broadly recommend the use of this method. Further research with improved methodological quality is necessary.
The Past, Current and Future Research in Cerebellar TMS Evoked Responses—A Narrative Review
Authors: Fong Po-Yu , Rothwell John C. , Rocchi Lorenzo
Published: April 2024

Transcranial magnetic stimulation coupled with electroencephalography (TMS-EEG) is a novel technique to investigate cortical physiology in health and disease. The cerebellum has recently gained attention as a possible new hotspot in the field of TMS-EEG, with several reports published recently. However, EEG responses obtained by cerebellar stimulation vary considerably across the literature, possibly due to different experimental methods. Compared to conventional TMS-EEG, which involves stimulation of the cortex, cerebellar TMS-EEG presents some technical difficulties, including strong muscle twitches in the neck area and a loud TMS click when double-cone coils are used, resulting in contamination of responses by electromyographic activity and sensory potentials. Understanding technical difficulties and limitations is essential for the development of cerebellar TMS-EEG research. In this review, we summarize findings of cerebellar TMS-EEG studies, highlighting limitations in experimental design and potential issues that can result in discrepancies between experimental outcomes. Lastly, we propose a possible direction for academic and clinical research with cerebellar TMS-EEG.
Combination of Two Long-Acting Antipsychotics in Schizophrenia Spectrum Disorders: A Systematic Review
Authors: Cipolla Salvatore , Catapano Pierluigi , D’Amico Daniela , Monda Rocchina , Sallusto Nunzia Paola , Perris Francesco , De Santis Valeria , Catapano Francesco , Luciano Mario , Fiorillo Andrea
Published: April 2024

Background: Up to 34% of patients with schizophrenia are resistant to several treatment trials. Lack of continuous and adequate treatment is associated with relapse, rehospitalization, a lower effect of antipsychotic therapy, and higher risk of side effects. Long-acting injectables antipsychotics (LAI APs) enhance compliance and improve clinical outcomes and quality of life in patients with schizophrenia, and thus it may be advisable to administer two LAI APs at the same time in cases of treatment-resistant schizophrenia. The purpose of this review is to summarize the available literature regarding the combined use of two LAI APs in patients with schizophrenia or other psychotic spectrum disorders. Methods: An extensive literature search for relevant articles regarding any combination of two long-acting injectable antipsychotics has been performed from inception up to 9 February 2024, on PubMed, Scopus and APA PsycInfo, according to the PRISMA statement. Only studies reporting combination of two LAI APs and its clinical outcome in patients with schizophrenia and related disorders were selected. Results: After the selection process, nine case reports, four case series and two observational retrospective studies were included in the final analysis. All patients treated with dual LAI APs reported a good response, and no new or unexpected adverse effects due to the combination of two LAIs were reported. Different drug combinations were used, and the most frequent association resulted in aripiprazole monohydrate + paliperidone palmitate once monthly (32 times). Conclusions: Our review highlights that the treatment regimen with two concurrent LAI APs is already widely used in clinical practice and is recognized as providing a promising, effective, and relatively safe therapeutic strategy for treating the schizophrenia spectrum disorders.
How Does Digital Leadership Foster Employee Innovative Behavior: A Cognitive–Affective Processing System Perspective
Authors: Gao Pengbin , Gao Yinghui
Published: April 2024

Employee innovative behavior is crucial for organizations to engage in innovative activities and gain competitive advantages in the context of digital transformation. Despite many studies having focused on the relationship between leadership and employee innovative behavior, the role of digital leadership and the underlying mechanisms for employee innovative behavior remain unclear. Using the cognitive–affective processing system framework, the study investigated the dual mediating role of psychological empowerment and affective commitment between digital leadership and employee innovative behavior and the moderating role of a proactive personality in such relationships. Employing data from 359 employees, the study conducted structure equation modeling to examine the hypotheses. The results show that digital leadership influences employee innovative behavior through psychological empowerment but not affective commitment. Furthermore, a proactive personality does not moderate the direct effect of digital leadership on psychological empowerment and affective commitment or the indirect effect of digital leadership on employee innovative behavior. Theoretical and practical implications are discussed.
Beyond Preservation: Heritage as an Educational Practice Process at 141 Neil Road, Singapore
Authors: Wei Xintong , Zhou Haoming , Joshi Nikhil , Tang Muyuan
Published: April 2024

Over the past two decades, the concept of heritage has been significantly broadened, with an increasing number of scholars viewing it not as a set of static objects with fixed meanings but as a social process consciously endowed with commemorative functions through human creation. This research illustrates and empirically supports this perspective through a case study. Initially, the paper reviews the development of the heritage concept through the literature review and elaborates on the idea of “heritage as process”. Subsequently, it examines Singapore’s enriching exploration and successful implementation of heritage conservation, particularly since 2000, when the local government shifted from preserving historical buildings to a comprehensive conservation strategy that embraces a sense of place, identity, and memory and encourages a bottom-up participatory approach. Finally, this paper takes the transformation of the century-old townhouse at 141 Neil Road in Singapore as its focus. After being renovated into an urban architectural heritage conservation laboratory, the house has gradually become a place of heritage on Neil Road by training professionals’ construction skills, transforming its function, introducing technological explorations, and enhancing public interaction. This process has developed the abilities of heritage conservation professionals in desktop research, reuse design, and practical construction skills while simultaneously promoting community participation and heightening awareness of heritage conservation among local residents. Through daily practices, this historical townhouse gradually became a place of heritage on Neil Road, exemplifying the research theme that “heritage is a comprehensive and dynamic practice encompassing social, cultural, and technological dimensions”. From being preserved for renovation in 2020 to being approved as a “Heritage Show House” by the Urban Redevelopment Authority (URA) in 2023, the case of 141 Neil Road offers a new perspective in the heritage conservation field that “heritage is always in the process of becoming, rather than a constant given”.
Barriers to Older Adults Adapting Smart Homes: Perceived Risk Scale Development
Authors: Liu Yuqi , Tamura Ryoichi , Xiao Liang
Published: April 2024

The 21st century has marked the dawn of an aging population. China’s aging process ranks first worldwide. The country has recognized the gravity of this demographic shift and implemented strategies to address it at the national level. A vast majority of elderly Chinese individuals (approximately 90%) aspire to age in their own homes. Smart homes, endowed with cutting-edge digital technologies, such as AI, the Internet of Things, and big data, hold vast potential for enabling this vision. However, acceptance of smart home products and services among elderly individuals in China remains low. The main reason is that the related products and services fail to effectively alleviate the perceived risk of this population in the R&D process of related products and services, and there is a lack of effective measurement methods. To holistically assess the potential obstacles faced by elderly individuals using smart home products and services, this study targeted individuals aged 45–60 years in China. This study aimed to develop a comprehensive perceived risk scale specific to smart homes for this demographic. Initially, this study identified key risk dimensions and corresponding measurement items through a rigorous literature review, user interviews, and expert consultations. Subsequently, it ensured the reliability and validity of each dimension and its corresponding observation variables through preliminary research, exploratory factor analysis, and confirmatory factor analysis. This approach allowed for a comprehensive understanding of the challenges faced by future elderly individuals when utilizing smart home products and services, thus enabling the development of more effective solutions. The scale encompassed ten factors and seventy measurement items, including Privacy and Security Risk (seven items), Physical Risk (seven items), Technological Risk (nine items), Performance Risk (seven items), Service Risk (nine items), Financial Risk (five items), Psychological Risk (seven items), Industry and Market Risk (six items), Social Support Risk (six items), and Policy and Legal risk (seven items). The measurement scale developed in this study represents a groundbreaking first attempt to create a systematic scale for assessing the perceived risks associated with smart homes for the elderly in China. It not only enables professionals, businesses, and manufacturers to avoid or reduce barriers in the R&D process of related products and services, facilitating smart home industry growth and enhancing user adoption, but also serves as a universal reference for the potential obstacles that digital technology may encounter in addressing aging-related issues, which has significant theoretical value and practical importance.
Experimental Research on the Floating Amount of Shield Tunnel Based on the Innovative Cumulative Floating Amount Calculation Method
Authors: Wu Jingrong , Chen Jing
Published: April 2024

The study of shield tunnel segment flotation is crucial for controlling the precision of underground excavation projects. Based on Winkler’s beam foundation theory, the load structure method, and the equivalent continuous beam model, and by considering the mechanical and spatial conditions that cause segment flotation, a novel theoretical calculation method for cumulative flotation is proposed using a simplified equivalent stiffness model of the tunnel. Additionally, a new concept of “equivalent flotation force” is introduced. The rationality and applicability of this theoretical calculation method are verified by comparing it with on-site construction data from the Yuanjiang River Crossing Tunnel Project in Changde, Hunan Province, China. The experimental results demonstrate that the theoretical calculation closely approximates the surface deformation monitoring data of the tunnel alignment in the eastern section of the project, and their deformation patterns are similar. Near the starting shaft, there is significant settlement influenced by stratum loss due to smaller tunnel flotation, with greater settlement occurring in the upper part. However, at approximately 45 m into both sections, they enter a deformation stability zone showing significant correlation in longitudinal deformation. Through comparison and verification of on-site experiments and theoretical model analysis, we preliminarily elucidate the feasibility of this innovative cumulative flotation theoretical calculation method which provides an important theoretical basis for assessing segment flotation issues in subsequent tunnel shield construction evaluations.
Architectural Analysis of the First Major Rehabilitation in the 21st Century of Olbrich’s Secession Building in Vienna
Authors: Arandelovic Biljana
Published: April 2024

The recent rehabilitation, reconstruction and adaptive reuse of Joseph Maria Olbrich’s Vienna Secession Building, completed in 2018, has brought the building into a contemporary age. This research article analyzes the only extensive rehabilitation carried out on the Secession Building so far in the 21st century. It studies what was accomplished during this specific rehabilitation process, and in particular emphasizes the reasons why such a process is crucial for culture heritage buildings in the city of Vienna. The purpose of this research is to evaluate the rehabilitation procedures used for the Secession Building and to identify any weaknesses to be resolved in the next rehabilitation. It provides an example of rehabilitation for any future similar initiatives, demonstrating both its positive and negative aspects.
A Study on the Regional Evolution of the Hefei Political and Cultural New District Skyline Based on Exploratory Factor Analysis and Semantic Segmentation
Authors: Zhang Le , Zhang Mengru , Yuan Mingxia , Guo Yanlong
Published: April 2024

The general population’s assessment and perception of a city can be influenced by its skyline, which is one of its representatives. This paper uses semantic segmentation and exploratory factor analysis to conduct a study from two different perspectives, aiming to analyse the development and current situation of the skyline of Hefei’s political and cultural new district. By collecting pictures of the skyline of the New Administrative and Cultural District, the New Administrative and Cultural District of Hefei’s skyline is being studied for overall changes using the semantic segmentation method, and to evaluate the quality of the skyline in various years, the entropy weight–TOPSIS is employed. Through the literature investigation and creation status of the new political and cultural district, the index system is scientifically formulated to conduct a questionnaire survey, and its reliability and validity are tested. This study is conducted with exploratory factor analysis of factors that are vital in the city skyline. The results of this study show that (1) after the semantic segmentation of the pictures, it is found that the proportion of buildings and the proportion of vegetation are both rising; the proportion of buildings tends to level off after 2018, and the proportion of vegetation shows a uniform increase. In the relative proximity ranking, the overall trend is increasing, and the skyline is ranked first in 2024. (2) Two variables were identified based on the findings of the exploratory factor analysis: ‘skyline value’ and ‘neighbourhood ecological quality’. The results of this study show that the skyline of Hefei’s New Administrative and Cultural District in 2024 is the best overall, and that the skyline of the New Administrative and Cultural District has developed into one of the city images of Hefei, and that its optimisation can be considered in terms of the “quality of the surrounding ecological environment”.
Tuning the Dynamic Thermal Parameters of Nanocarbon Ionanofluids: A Photopyroelectric Study
Authors: Swapna Mohanachandran Nair Sindhu , Tripon Carmen , Farcas Alexandra , Dadarlat Dorin Nicolae , Korte Dorota , Sankararaman Sankaranarayana Iyer
Published: April 2024

The present work delineates the tailoring of the thermal effusivity and diffusivity of the novel class of heat transfer fluids—ionanofluids—by the incorporation of nanocarbons like diesel soot (DS), camphor soot (CS), carbon nanotubes (CN), and graphene (GR). When the thermal diffusivity delivers information on the thermal energy propagation, the thermal effusivity concerns the energy exchange at the interface, enabling energy-efficient thermal system design. The nanocarbons are subjected to morphological characterisation by field emission scanning electron microscopy. Fourier-transform infrared and Raman spectroscopic analyses confirm functional groups and vibrational bands. The microcrystalline size and graphiticity are also understood from the Raman spectrum. Ionanofluids prepared by dispersing nanocarbons into an ionic liquid base 1-Butyl-3-methylimidazolium methyl sulfate (BMMS) are analysed by nondestructive photopyroelectric calorimetry (PPE). The PPE analysis of ionanofluids demonstrates that nanocarbons influence thermal parameters in the base fluid, with soot ionanofluids exhibiting increased thermal effusivity and diffusivity due to their various carbon allotropic composition. This study underscores the importance of selecting the appropriate carbon allotrope for tailoring ionanofluids’ thermal properties, providing insights into manipulating these properties for enhanced performance across various industrial applications.
Temporal Muscle Thickness Compared to Functional Scales as a Prognostic Parameter in Patients with Brain Metastases
Authors: Klingenschmid Julia , Krigers Aleksandrs , Pinggera Daniel , Kerschbaumer Johannes , Pichler Nadine , Schoen Victoria , Demetz Matthias , Grams Astrid E. , Thomé Claudius , Freyschlag Christian F.
Published: April 2024

Metastases are the most frequent intracranial malignant tumors in adults. While Karnofsky Performance Status (KPS) and Clinical Frailty Scale (CFS) are known to have significant impact on overall survival (OS), temporal muscle thickness (TMT) has been postulated to be a promising new parameter to estimate prognosis. Patients who received a resection of one to three brain metastases in our institution were included. Temporal muscle thickness was measured in preoperative MRI scans according to a standardized protocol. In 199 patients, the mean TMT was 7.5 mm (95CI 7.3–7.7) and the mean OS during follow-up was 31.3 months (95CI 24.2–38.3). There was no significant correlation of TMT and preoperative or follow-up CFS and KPS. While CFS and KPS did significantly correlate with OS (p < 0.001 for each), no correlation was demonstrated for TMT. CFS showed a superior prognostic value compared to KPS. TMT failed to show a significant impact on OS or patient performance, whereas the clinical scales (KPS and CFS) demonstrate a good correlation with OS. Due to its superiority over KPS, we strongly recommend the use of CFS to estimate OS in patients with brain metastases.
Association between Periodontitis and Hematologic Cancer: An NHIRD Cohort Study in Taiwan
Authors: Huang Liang-Gie , Yu Cheng-Chia , Lin Ming-Ching , Wang Yu-Hsun , Chang Yu-Chao
Published: April 2024

Background: Chronic periodontitis, an inflammation-related disorder affecting global populations, has been revealed to be linked to diverse cancers. Numerous epidemiological studies have not shown a link between chronic periodontitis and blood cancers in Taiwan. Methods: This study included 601,628 patients, diagnosed with newly chronic periodontitis by the ICD-9-CM classification, who were enrolled from 2001 to 2021 in the National Health Insurance Research Database (NHIRD) in Taiwan. In this study, we employed comprehensive statistical analyses to investigate the association between chronic periodontitis and hematologic cancers. Initially, we calculated incidence density and used a Poisson regression to analyze relative risk. Subsequently, we compared the cumulative incidence of hematological cancer in both chronic and non-chronic periodontitis groups using the Kaplan–Meier method. Results: The results revealed a significantly lower cumulative incidence of hematologic cancer in individuals with non-chronic periodontitis over a 12-year follow-up period. To further explore the risk factors, a Cox proportional hazard regression analysis was conducted. Being male (adjusted hazard ratio [aHR] = 1.21, 95% CI: 1.04 to 1.42; p = 0.014) and having hypertension (aHR = 1.34, 95% CI: 1.06 to 1.69; p = 0.015) were demonstrated to be associated with an increased risk of hematologic cancers, respectively. In addition, in a subtype multivariate analysis for categorizing hematologic cancers into lymphoma and leukemia, the aHR for leukemia was 1.48 (95% CI: 1.13 to 1.93; p = 0.004) and aHR for lymphoma was 1.15 (95% CI: 0.96 to 1.37; p = 0.140). Conclusions: This study found that being male and having hypertension were the significant risk factors for hematological malignancies. Moreover, the association between chronic periodontitis and specific subtypes of hematologic cancers was confirmed.
COVID-19 and Cancer Detection in Russia
Authors: Sudarikov Andrey
Published: April 2024

Overdiagnosis, associated with mass testing in healthy populations, is a significant issue for breast, prostate, renal, and thyroid cancers. During the lockdowns caused by the COVID-19 pandemic, the intensity of cancer screening was expected to go down. In this study, we analyzed the impact of the expected reduction in screening intensity on morbidity and mortality from certain malignancies. Cumulative data from the Russian National Cancer Registry available from 2000 to 2022 were analyzed. It was noted that there has been no noticeable effect of the COVID-19 lockdowns on mortality rates from breast, prostate, renal, or thyroid cancers. At the same time, the detectable incidence decreased markedly in 2020 at the time of the lockdowns and then returned to pre-pandemic levels in 2022. At the moment, there is no sufficient reason to believe that skipping screening tests in 2020 could have any impact on breast, prostate, renal, or thyroid cancer mortality two years later (2022). The data presented further confirm that the overdiagnosis of these types of malignancies is caused by widespread screening among a generally healthy population.
Proteins Involved in Focal Cell Adhesion and Podosome Formation Are Differentially Expressed during Colorectal Tumorigenesis in AOM-Treated Rats
Authors: Swain Ian X. , Kresak Adam M.
Published: April 2024

Colorectal tumorigenesis involves the development of aberrant crypt foci (ACF) or preneoplastic lesions, representing the earliest morphological lesion visible in colon cancer. The purpose of this study was to determine changes in protein expression in carcinogen-induced ACF as they mature and transform into adenomas. Protein expression profiles of azoxymethane (AOM)-induced F344 rat colon ACF and adenomas were compared at four time points, 4 (control), 8, 16, and 24 weeks post AOM administration (n = 9/group), with time points correlating with induction and transformation events. At each time point, micro-dissected ACF and/or adenoma tissues were analyzed across multiple quantitative two-dimensional (2D-DIGE) gels using a Cy-dye labeling technique and a pooled internal standard to quantify expression changes with statistical confidence. Western blot and subsequent network pathway mapping were used to confirm and elucidate differentially expressed (p ≤ 0.05) proteins, including changes in vinculin (Vcl; p = 0.007), scinderin (Scin; p = 0.02), and profilin (Pfn1; p = 0.01), By determining protein expression changes in ACF as they mature and transform into adenomas, a “baseline” of altered regulatory proteins associated with adenocarcinoma development in this model has been elucidated. These data will enable future studies aimed at biomarker identification and understanding the molecular biology of intestinal tumorigenesis and adenocarcinoma maturation under varying intestinal conditions.
Extracellular Vesicles for Childhood Cancer Liquid Biopsy
Authors: Singhto Nilubon , Pongphitcha Pongpak , Jinawath Natini , Hongeng Suradej , Chutipongtanate Somchai
Published: April 2024

Liquid biopsy involves the utilization of minimally invasive or noninvasive techniques to detect biomarkers in biofluids for disease diagnosis, monitoring, or guiding treatments. This approach is promising for the early diagnosis of childhood cancer, especially for brain tumors, where tissue biopsies are more challenging and cause late detection. Extracellular vesicles offer several characteristics that make them ideal resources for childhood cancer liquid biopsy. Extracellular vesicles are nanosized particles, primarily secreted by all cell types into body fluids such as blood and urine, and contain molecular cargos, i.e., lipids, proteins, and nucleic acids of original cells. Notably, the lipid bilayer-enclosed structure of extracellular vesicles protects their cargos from enzymatic degradation in the extracellular milieu. Proteins and nucleic acids of extracellular vesicles represent genetic alterations and molecular profiles of childhood cancer, thus serving as promising resources for precision medicine in cancer diagnosis, treatment monitoring, and prognosis prediction. This review evaluates the recent progress of extracellular vesicles as a liquid biopsy platform for various types of childhood cancer, discusses the mechanistic roles of molecular cargos in carcinogenesis and metastasis, and provides perspectives on extracellular vesicle-guided therapeutic intervention. Extracellular vesicle-based liquid biopsy for childhood cancer may ultimately contribute to improving patient outcomes.
Three-Dimensional Mesoporous Ni-CeO2 Catalyst for Dry Reforming of Methane
Authors: Jin Huiyao , Liu Yuanqiao , Huang Lizhi , Liu Yali , Cui Sha , Liu Hui , Xu Jing , Wang Luhui
Published: April 2024

Using the colloidal solution combustion approach, a three-dimensional mesoporous 5%Ni-CeO2-M catalyst was developed, with Ni incorporated into the pores, and applied in the dry reforming of methane. Comprehensive characterization revealed that the 5%Ni-CeO2-M catalyst had a large specific surface area and a three-dimensional mesoporous structure. A rich Ni-CeO2 interface was formed by closely spaced tiny CeO2 and NiO nanoparticles within the spherical pore wall. With very little carbon deposition over a 100 h period at 700 °C, the catalyst showed excellent activity and stability. The tiny Ni nanoparticles, along with the substantial Ni-CeO2 interfaces that make up this three-dimensional in-form mesoporous catalyst, are responsible for the outstanding effectiveness of this 5%Ni-CeO2-M catalyst.
Clinical Phenotypes, Serological Biomarkers, and Synovial Features Defining Seropositive and Seronegative Rheumatoid Arthritis: A Literature Review
Authors: Perera James , Delrosso Chiara Aurora , Nerviani Alessandra , Pitzalis Costantino
Published: April 2024

Rheumatoid arthritis (RA) is a chronic autoimmune disorder which can lead to long-term joint damage and significantly reduced quality of life if not promptly diagnosed and adequately treated. Despite significant advances in treatment, about 40% of patients with RA do not respond to individual pharmacological agents and up to 20% do not respond to any of the available medications. To address this large unmet clinical need, several recent studies have focussed on an in-depth histological and molecular characterisation of the synovial tissue to drive the application of precision medicine to RA. Currently, RA patients are clinically divided into “seropositive” or “seronegative” RA, depending on the presence of routinely checked antibodies. Recent work has suggested that over the last two decades, long-term outcomes have improved significantly in seropositive RA but not in seronegative RA. Here, we present up-to-date differences in epidemiology, clinical features, and serological biomarkers in seronegative versus seropositive RA and discuss how histological and molecular synovial signatures, revealed by recent large synovial biopsy-based clinical trials, may be exploited to refine the classification of RA patients, especially in the seronegative group.
Induced Pluripotent Stem Cells and Organoids in Advancing Neuropathology Research and Therapies
Authors: Pazzin Douglas , Previato Thales , Budelon Gonçalves João , Zanirati Gabriele , Xavier Fernando , da Costa Jaderson , Marinowic Daniel
Published: April 2024

This review delves into the groundbreaking impact of induced pluripotent stem cells (iPSCs) and three-dimensional organoid models in propelling forward neuropathology research. With a focus on neurodegenerative diseases, neuromotor disorders, and related conditions, iPSCs provide a platform for personalized disease modeling, holding significant potential for regenerative therapy and drug discovery. The adaptability of iPSCs, along with associated methodologies, enables the generation of various types of neural cell differentiations and their integration into three-dimensional organoid models, effectively replicating complex tissue structures in vitro. Key advancements in organoid and iPSC generation protocols, alongside the careful selection of donor cell types, are emphasized as critical steps in harnessing these technologies to mitigate tumorigenic risks and other hurdles. Encouragingly, iPSCs show promising outcomes in regenerative therapies, as evidenced by their successful application in animal models.
CD4+ T-Cell Senescence in Neurodegenerative Disease: Pathogenesis and Potential Therapeutic Targets
Authors: Gao Yan , Lu Yaoping , Liang Xiaojing , Zhao Mengwei , Yu Xinyue , Fu Haiying , Yang Wei
Published: April 2024

With the increasing proportion of the aging population, neurodegenerative diseases have become one of the major health issues in society. Neurodegenerative diseases (NDs), including multiple sclerosis (MS), Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral sclerosis (ALS), are characterized by progressive neurodegeneration associated with aging, leading to a gradual decline in cognitive, emotional, and motor functions in patients. The process of aging is a normal physiological process in human life and is accompanied by the aging of the immune system, which is known as immunosenescence. T-cells are an important part of the immune system, and their senescence is the main feature of immunosenescence. The appearance of senescent T-cells has been shown to potentially lead to chronic inflammation and tissue damage, with some studies indicating a direct link between T-cell senescence, inflammation, and neuronal damage. The role of these subsets with different functions in NDs is still under debate. A growing body of evidence suggests that in people with a ND, there is a prevalence of CD4+ T-cell subsets exhibiting characteristics that are linked to senescence. This underscores the significance of CD4+ T-cells in NDs. In this review, we summarize the classification and function of CD4+ T-cell subpopulations, the characteristics of CD4+ T-cell senescence, the potential roles of these cells in animal models and human studies of NDs, and therapeutic strategies targeting CD4+ T-cell senescence.
Allogeneic Stem Cell Transplantation in Refractory Acute Myeloid Leukaemia
Authors: Bono Roberto , Sapienza Giuseppe , Tringali Stefania , Rotolo Cristina , Patti Caterina , Mulè Antonino , Calafiore Valeria , Santoro Alessandra , Castagna Luca
Published: April 2024

Refractory acute myeloid leukaemia is very difficult to treat and represents an unmet clinical need. In recent years, new drugs and combinations of drugs have been tested in this category, with encouraging results. However, all treated patients relapsed and died from the disease. The only curative option is allogeneic transplantation through a graft from a healthy donor immune system. Using myeloablative conditioning regimens, the median overall survival regimens is 19%. Several so-called sequential induction chemotherapies followed by allogeneic transplantation conditioned by reduced intensity regimens have been developed, improving the overall survival to 25–57%. In the allogeneic transplantation field, continuous improvements in practices, particularly regarding graft versus host disease prevention, infection prevention, and treatment, have allowed us to observe improvements in survival rates. This is true mainly for patients in complete remission before transplantation and less so for refractory patients. However, full myeloablative regimens are toxic and carry a high risk of treatment-related mortality. In this review, we describe the results obtained with the different modalities used in more recent retrospective and prospective studies. Based on these findings, we speculate how allogeneic stem cell transplantation could be modified to maximise its therapeutic effect on refractory acute myeloid leukaemia.
Identifying Opportunities for Early Detection of Cerebral Palsy
Authors: Hornby Brittany , Paleg Ginny S. , Williams Sîan A. , Hidalgo-Robles Álvaro , Livingstone Roslyn W. , Montufar Wright Parma E. , Taylor Alice , Shrader Michael Wade
Published: April 2024

This study aimed to evaluate assessment and referral practices for the early detection and diagnosis of children at risk for or with cerebral palsy (CP) by health care and education providers in Maryland and Delaware. A secondary aim was to identify barriers for using early detection tools and identify opportunities for change to support early diagnosis and improve care. Seventy-two participants answered ≥ 50% of the survey questions. Most were occupational or physical therapists (86%) working in early intervention (61%). Eighty-eight percent indicated awareness that CP can be diagnosed by 12 months. Though 86% stated they typically suspect a diagnosis of CP between 0 and 12 months, only 19% reported that their patients received a CP diagnosis < 12 months. The Developmental Assessment of Young Children (73%) and the Peabody Developmental Motor Scales-2 (59%) were used most. Many respondents indicated never using magnetic resonance imaging (70%), the General Movements Assessment (87%), or the Hammersmith Infant Neurological Exam (69%). Participants identified clinical signs and symptoms prompting a referral for the diagnostic assessment of CP, most commonly stiffness in legs (95%), excessive head lag (93%), and persistent fisting (92%). Policy and organizational change, clinician education, and training are needed to support the implementation of CP early detection guidelines.