bluuebunny/crossref_metadata_2025 huggingface dataset:
Canonical Wnt Pathway Is Involved in Chemoresistance and Cell Cycle Arrest Induction in Colon Cancer Cell Line Spheroids
Authors: Moreno-Londoño Angela Patricia , Castañeda-Patlán María Cristina , Sarabia-Sánchez Miguel Angel , Macías-Silva Marina , Robles-Flores Martha
Published: March 2023

The presence of cancer stem cells (CSCs) has been associated with the induction of drug resistance and disease recurrence after therapy. 5-Fluorouracil (5FU) is widely used as the first-line treatment of colorectal cancer (CRC). However, its effectiveness may be limited by the induction of drug resistance in tumor cells. The Wnt pathway plays a key role in the development and CRC progression, but it is not clearly established how it is involved in CSCs resistance to treatment. This work aimed to investigate the role played by the canonical Wnt/β-catenin pathway in CSCs resistance to 5FU treatment. Using tumor spheroids as a model of CSCs enrichment of CRC cell lines with different Wnt/β-catenin contexts, we found that 5FU induces in all CRC spheroids tested cell death, DNA damage, and quiescence, but in different proportions for each one: RKO spheroids were very sensitive to 5FU, while SW480 were less susceptible, and the SW620 spheroids, the metastatic derivative of SW480 cells, displayed the highest resistance to death, high clonogenic capacity, and the highest ability for regrowth after 5FU treatment. Activating the canonical Wnt pathway with Wnt3a in RKO spheroids decreased the 5FU-induced cell death. But the Wnt/β-catenin pathway inhibition with Adavivint alone or in combination with 5FU in spheroids with aberrant activation of this pathway produced a severe cytostatic effect compromising their clonogenic capacity and diminishing the stem cell markers expression. Remarkably, this combined treatment also induced the survival of a small cell subpopulation that could exit the arrest, recover SOX2 levels, and re-grow after treatment.
Pentacoordinated Organotin(IV) Complexes as an Alternative in the Design of Highly Efficient Optoelectronic and Photovoltaic Devices: Synthesis and Photophysical Characterization
Authors: Sánchez Vergara María Elena , Gómez Elizabeth , Toledo Dircio Emiliano , Álvarez Bada José Ramón , Cuenca Pérez Samuel , Galván Hidalgo José Miguel , González Hernández Arturo , Hernández Ortega Simón
Published: March 2023

The synthesis of four pentacoordinated organotin(IV) complexes prepared in a one-pot reaction from 2-hydroxy-1-naphthaldehyde, 2-amino-3-hydroxypyridine and organotin oxides is reported. The complexes were characterized by UV-Vis, IR, MS, 1H, 13C and 119Sn NMR techniques. The compound based on 2,2-diphenyl-6-aza-1,3-dioxa-2-stannanaphtho[1,2-h]pyrido[3,2-d]cyclononene revealed the formation of a monomeric complex with a distorted five-coordinated molecular geometry intermediate between the trigonal bipyramidal and square pyramidal. In order to find possible applications in photovoltaic devices, hybrid films of organotin(IV) complexes embedded in poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) with graphene were deposited. The topographic and mechanical properties were examined. The film with the complex integrated into the cyclohexyl substituent has high plastic deformation, with a maximum stress of 1.69 × 107 Pa and a Knoop hardness of 0.061. The lowest values of 1.85 eV for the onset gap and 3.53 eV for the energy gap were obtained for the heterostructure having the complex with the phenyl substituent. Bulk heterojunction devices were fabricated; these devices showed ohmic behavior at low voltages and a space-charge-limited current (SCLC) conduction mechanism at higher voltages. A value of 0.02 A was found for the maximum carried current. The SCLC mechanism suggests hole mobility values of between 2.62 × 10−2 and 3.63 cm2/V.s and concentrations of thermally excited holes between 2.96 × 1018 and 4.38 × 1018 m−3.
Cathepsin H Knockdown Reverses Radioresistance of Hepatocellular Carcinoma via Metabolic Switch Followed by Apoptosis
Authors: Chen Qiao , Qu Shugen , Liang Zhenzhen , Liu Yi , Chen Huajian , Ma Shumei , Liu Xiaodong
Published: March 2023

Despite the wide application of radiotherapy in HCC, radiotherapy efficacy is sometimes limited due to radioresistance. Although radioresistance is reported with high glycolysis, the underlying mechanism between radioresistance and cancer metabolism, as well as the role of cathepsin H (CTSH) within it, remain unclear. In this study, tumor-bearing models and HCC cell lines were used to observe the effect of CTSH on radioresistance. Proteome mass spectrometry, followed by enrichment analysis, were used to investigate the cascades and targets regulated by CTSH. Technologies such as immunofluorescence co-localization flow cytometry and Western blot were used for further detection and verification. Through these methods, we originally found CTSH knockdown (KD) perturbed aerobic glycolysis and enhanced aerobic respiration, and thus promoted apoptosis through up-regulation and the release of proapoptotic factors such as AIFM1, HTRA2, and DIABLO, consequently reducing radioresistance. We also found that CTSH, together with its regulatory targets (such as PFKL, HK2, LDH, and AIFM1), was correlated with tumorigenesis and poor prognosis. In summary, our study found that the cancer metabolic switch and apoptosis were regulated by CTSH signaling, leading to the occurrence of radioresistance in HCC cells and suggesting the potential value of HCC diagnosis and therapy.
Overexpression of a Fragaria vesca 1R-MYB Transcription Factor Gene (FvMYB114) Increases Salt and Cold Tolerance in Arabidopsis thaliana
Authors: Li Wenhui , Li Peng , Chen Huiyun , Zhong Jiliang , Liang Xiaoqi , Wei Yangfan , Zhang Lihua , Wang Haibo , Han Deguo
Published: March 2023

The MYB (v-MYB avian myeloblastosis viral oncogene homolog) transcription factor (TF) family has numerous members with complex and diverse functions, which play an indispensable role in regulating the response of plants to stress. In this study, a new 1R-MYB TF gene was obtained from Fragaria vesca (a diploid strawberry) by cloning technology and given a new name, FvMYB114. According to the subcellular localization results, FvMYB114 protein was a nuclear localization protein. Overexpression of FvMYB114 greatly enhanced the adaptability and tolerance of Arabidopsis thaliana to salt and low temperature. Under salt and cold stress, the transgenic plants had greater proline and chlorophyll contents and higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than the wild-type (WT) and unloaded-line (UL) A. thaliana. However, malondialdehyde (MDA) was higher in the WT and UL lines. These results suggested that FvMYB114 may be involved in regulating the response of A. thaliana to salt stress and cold stress. FvMYB114 can also promote the expression of genes, such as the genes AtSOS1/3, AtNHX1 and AtLEA3 related to salt stress and the genes AtCCA1, AtCOR4 and AtCBF1/3 related to cold stress, further improving the tolerance of transgenic plants to salt and cold stress.
Role of Defensins in Tumor Biology
Authors: Adyns Lowie , Proost Paul , Struyf Sofie
Published: March 2023

Defensins have long been considered as merely antimicrobial peptides. Throughout the years, more immune-related functions have been discovered for both the α-defensin and β-defensin subfamily. This review provides insights into the role of defensins in tumor immunity. Since defensins are present and differentially expressed in certain cancer types, researchers started to unravel their role in the tumor microenvironment. The human neutrophil peptides have been demonstrated to be directly oncolytic by permealizing the cell membrane. Further, defensins can inflict DNA damage and induce apoptosis of tumor cells. In the tumor microenvironment, defensins can act as chemoattractants for subsets of immune cells, such as T cells, immature dendritic cells, monocytes and mast cells. Additionally, by activating the targeted leukocytes, defensins generate pro-inflammatory signals. Moreover, immuno-adjuvant effects have been reported in a variety of models. Therefore, the action of defensins reaches beyond their direct antimicrobial effect, i.e., the lysis of microbes invading the mucosal surfaces. By causing an increase in pro-inflammatory signaling events, cell lysis (generating antigens) and attraction and activation of antigen presenting cells, defensins could have a relevant role in activating the adaptive immune system and generating anti-tumor immunity, and could thus contribute to the success of immune therapy.
198Au-Coated Superparamagnetic Iron Oxide Nanoparticles for Dual Magnetic Hyperthermia and Radionuclide Therapy of Hepatocellular Carcinoma
Authors: Gharibkandi Nasrin Abbasi , Żuk Michał , Muftuler Fazilet Zumrut Biber , Wawrowicz Kamil , Żelechowska-Matysiak Kinga , Bilewicz Aleksander
Published: March 2023

This study was performed to synthesize a radiopharmaceutical designed for multimodal hepatocellular carcinoma (HCC) treatment involving radionuclide therapy and magnetic hyperthermia. To achieve this goal, the superparamagnetic iron oxide (magnetite) nanoparticles (SPIONs) were covered with a layer of radioactive gold (198Au) creating core–shell nanoparticles (SPION@Au). The synthesized SPION@Au nanoparticles exhibited superparamagnetic properties with a saturation magnetization of 50 emu/g, which is lower than reported for uncoated SPIONs (83 emu/g). Nevertheless, the SPION@Au core–shell nanoparticles showed a sufficiently high saturation magnetization value which allows them to reach a temperature of 43 °C at a magnetic field frequency of 386 kHz. The cytotoxic effect of nonradioactive and radioactive SPION@Au–polyethylene glycol (PEG) bioconjugates was carried out by treating HepG2 cells with various concentrations (1.25–100.00 µg/mL) of the compound and radioactivity in range of 1.25–20 MBq/mL. The moderate cytotoxic effect of nonradioactive SPION@Au-PEG bioconjugates on HepG2 was observed. The cytotoxic effect associated with the β− radiation emitted by 198Au was much greater and already reaches a cell survival fraction below 8% for 2.5 MBq/mL of radioactivity after 72 h. Thus, the killing of HepG2 cells in HCC therapy should be possible due to the combination of the heat-generating properties of the SPION-198Au–PEG conjugates and the radiotoxicity of the radiation emitted by 198Au.
Establishing Co-Culture Blood–Brain Barrier Models for Different Neurodegeneration Conditions to Understand Its Effect on BBB Integrity
Authors: Park Jun Sung , Choe Kyonghwan , Khan Amjad , Jo Myeung Hoon , Park Hyun Young , Kang Min Hwa , Park Tae Ju , Kim Myeong Ok
Published: March 2023

The blood–brain barrier (BBB) is a functional interface that provides selective permeability, protection from toxic substances, transport of nutrients, and clearance of brain metabolites. Additionally, BBB disruption has been shown to play a role in many neurodegenerative conditions and diseases. Therefore, the aim of this study was to establish a functional, convenient, and efficient in vitro co-cultured BBB model that can be used for several physiological conditions related to BBB disruption. Mouse brain-derived endothelial (bEnd.3) and astrocyte (C8-D1A) cells were co-cultured on transwell membranes to establish an intact and functional in vitro model. The co-cultured model and its effects on different neurological diseases and stress conditions, including Alzheimer’s disease (AD), neuroinflammation, and obesity, have been examined by transendothelial electrical resistance (TEER), fluorescein isothiocyanate (FITC) dextran, and tight junction protein analyses. Scanning electron microscope images showed evidence of astrocyte end-feet processes passing through the membrane of the transwell. Moreover, the co-cultured model showed effective barrier properties in the TEER, FITC, and solvent persistence and leakage tests when compared to the mono-cultured model. Additionally, the immunoblot results showed that the expression of tight junction proteins such as zonula occludens-1 (ZO-1), claudin-5, and occludin-1 was enhanced in the co-culture. Lastly, under disease conditions, the BBB structural and functional integrity was decreased. The present study demonstrated that the co-cultured in vitro model mimicked the BBB’s structural and functional integrity and, under disease conditions, the co-cultured model showed similar BBB damages. Therefore, the present in vitro BBB model can be used as a convenient and efficient experimental tool to investigate a wide range of BBB-related pathological and physiological studies.
Contrasting Metabolisms in Green and White Leaf Sectors of Variegated Pelargonium zonale—An Integrative Transcriptomic and Metabolomic Study
Authors: Milić Dejana , Pantelić Ana , Banović Đeri Bojana , Samardžić Jelena , Vidović Marija
Published: March 2023

The photosynthetically active green leaf (GL) and non-active white leaf (WL) tissues of variegated Pelargonium zonale provide an excellent model system for studying processes associated with photosynthesis and sink-source interactions, enabling the same microenvironmental conditions. By combining differential transcriptomics and metabolomics, we identified the main differences between these two metabolically contrasting tissues. Genes related to photosynthesis and associated pigments, the Calvin–Benson cycle, fermentation, and glycolysis were strongly repressed in WL. On the other hand, genes related to nitrogen and protein metabolism, defence, cytoskeletal components (motor proteins), cell division, DNA replication, repair and recombination, chromatin remodelling, and histone modifications were upregulated in WL. A content of soluble sugars, TCA intermediates, ascorbate, and hydroxybenzoic acids was lower, while the concentration of free amino acids (AAs), hydroxycinnamic acids, and several quercetin and kaempferol glycosides was higher in WL than in GL. Therefore, WL presents a carbon sink and depends on photosynthetic and energy-generating processes in GL. Furthermore, the upregulated nitrogen metabolism in WL compensates for the insufficient energy from carbon metabolism by providing alternative respiratory substrates. At the same time, WL serves as nitrogen storage. Overall, our study provides a new genetic data resource for the use of this excellent model system and for ornamental pelargonium breeding and contributes to uncovering molecular mechanisms underlying variegation and its adaptive ecological value.
Discrimination of Olive Oil and Extra-Virgin Olive Oil from Other Vegetable Oils by Targeted and Untargeted HRMS Profiling of Phenolic and Triterpenic Compounds Combined with Chemometrics
Authors: Geana Elisabeta-Irina , Ciucure Corina Teodora , Apetrei Irina Mirela , Clodoveo Maria Lisa , Apetrei Constantin
Published: March 2023

Extra-virgin olive oil (EVOO) and virgin olive oil (VOO) are valuable natural products of great economic interest for their producing countries, and therefore, it is necessary to establish methods capable of proving the authenticity of these oils on the market. This work presents a methodology for the discrimination of olive oil and extra-virgin olive oil from other vegetable oils based on targeted and untargeted high-resolution mass spectrometry (HRMS) profiling of phenolic and triterpenic compounds coupled with multivariate statistical analysis of the data. Some phenolic compounds (cinnamic acid, coumaric acids, apigenin, pinocembrin, hydroxytyrosol and maslinic acid), secoiridoids (elenolic acid, ligstroside and oleocanthal) and lignans (pinoresinol and hydroxy and acetoxy derivatives) could be olive oil biomarkers, whereby these compounds are quantified in higher amounts in EVOO compared to other vegetable oils. The principal component analysis (PCA) performed based on the targeted compounds from the oil samples confirmed that cinnamic acid, coumaric acids, apigenin, pinocembrin, hydroxytyrosol and maslinic acid could be considered as tracers for olive oils authentication. The heat map profiles based on the untargeted HRMS data indicate a clear discrimination of the olive oils from the other vegetable oils. The proposed methodology could be extended to the authentication and classification of EVOOs depending on the variety, geographical origin, or adulteration practices.
Let’s Go 3D! New Generation of Models for Evaluating Drug Response and Resistance in Prostate Cancer
Authors: Petrić Tina , Sabol Maja
Published: March 2023

Prostate cancer (PC) is the third most frequently diagnosed cancer worldwide and the second most frequent in men. Several risk factors can contribute to the development of PC, and those include age, family history, and specific genetic mutations. So far, drug testing in PC, as well as in cancer research in general, has been performed on 2D cell cultures. This is mainly because of the vast benefits these models provide, including simplicity and cost effectiveness. However, it is now known that these models are exposed to much higher stiffness; lose physiological extracellular matrix on artificial plastic surfaces; and show changes in differentiation, polarization, and cell–cell communication. This leads to the loss of crucial cellular signaling pathways and changes in cell responses to stimuli when compared to in vivo conditions. Here, we emphasize the importance of a diverse collection of 3D PC models and their benefits over 2D models in drug discovery and screening from the studies done so far, outlining their benefits and limitations. We highlight the differences between the diverse types of 3D models, with the focus on tumor–stroma interactions, cell populations, and extracellular matrix composition, and we summarize various standard and novel therapies tested on 3D models of PC for the purpose of raising awareness of the possibilities for a personalized approach in PC therapy.
Epigenetic Regulation Mediated by Sphingolipids in Cancer
Authors: Bozzini Nicolò , Avnet Sofia , Baldini Nicola , Cortini Margherita
Published: March 2023

Epigenetic changes are heritable modifications that do not directly affect the DNA sequence. In cancer cells, the maintenance of a stable epigenetic profile can be crucial to support survival and proliferation, and said profile can differ significantly from that of healthy cells. The epigenetic profile of a cancer cell can be modulated by several factors, including metabolites. Recently, sphingolipids have emerged as novel modulators of epigenetic changes. Ceramide and sphingosine 1-phosphate have become well known in cancer due to activating anti-tumour and pro-tumour signalling pathways, respectively, and they have recently been shown to also induce several epigenetic modifications connected to cancer growth. Additionally, acellular factors in the tumour microenvironment, such as hypoxia and acidosis, are now recognised as crucial in promoting aggressiveness through several mechanisms, including epigenetic modifications. Here, we review the existing literature on sphingolipids, cancer, and epigenetic changes, with a focus on the interaction between these elements and components of the chemical tumour microenvironment.
Identification and Empiric Evaluation of New Inhibitors of the Multidrug Transporter P-Glycoprotein (ABCB1)
Authors: Cheema Yasmeen , Kiani Yusra Sajid , Linton Kenneth J. , Jabeen Ishrat
Published: March 2023

The expression of the drug efflux pump ABCB1 correlates negatively with cancer survival, making the transporter an attractive target for therapeutic inhibition. In order to identify new inhibitors of ABCB1, we have exploited the cryo-EM structure of the protein to develop a pharmacophore model derived from the best docked conformations of a structurally diverse range of known inhibitors. The pharmacophore model was used to screen the Chembridge compound library. We identified six new potential inhibitors with distinct chemistry compared to the third-generation inhibitor tariquidar and with favourable lipophilic efficiency (LipE) and lipophilicity (CLogP) characteristics, suggesting oral bioavailability. These were evaluated experimentally for efficacy and potency using a fluorescent drug transport assay in live cells. The half-maximal inhibitory concentrations (IC50) of four of the compounds were in the low nanomolar range (1.35 to 26.4 nM). The two most promising compounds were also able to resensitise ABCB1-expressing cells to taxol. This study demonstrates the utility of cryo-electron microscopy structure determination for drug identification and design.
Enhancing Small Medical Dataset Classification Performance Using GAN
Authors: Alauthman Mohammad , Al-qerem Ahmad , Sowan Bilal , Alsarhan Ayoub , Eshtay Mohammed , Aldweesh Amjad , Aslam Nauman
Published: March 2023

Developing an effective classification model in the medical field is challenging due to limited datasets. To address this issue, this study proposes using a generative adversarial network (GAN) as a data-augmentation technique. The research aims to enhance the classifier’s generalization performance, stability, and precision through the generation of synthetic data that closely resemble real data. We employed feature selection and applied five classification algorithms to thirteen benchmark medical datasets, augmented using the least-square GAN (LS-GAN). Evaluation of the generated samples using different ratios of augmented data showed that the support vector machine model outperforms other methods with larger samples. The proposed data augmentation approach using a GAN presents a promising solution for enhancing the performance of classification models in the healthcare field.
Value of Right and Left Ventricular T1 and T2 Blood Pool Mapping in Patients with Chronic Thromboembolic Hypertension before and after Balloon Pulmonary Angioplasty
Authors: Roller Fritz C. , Schüßler Armin , Kremer Nils , Harth Sebastian , Kriechbaum Steffen D. , Wiedenroth Christoph B. , Guth Stefan , Breithecker Andreas , Richter Manuel , Tello Khodr , Seeger Werner , Mayer Eckhard , Krombach Gabriele A.
Published: March 2023

Background: Parametric imaging has taken a steep rise in recent years and non-cardiac applications are of increasing interest. Therefore, the aim of our study was to assess right (RV) and left ventricular (LV) blood pool T1 and T2 values in patients with chronic thromboembolic pulmonary hypertension (CTEPH) compared to control subjects and their correlation to pulmonary hemodynamic. Methods: 26 patients with CTEPH (mean age 64.8 years ± 12.8 SD; 15 female), who underwent CMR and right heart catheterization (RHC) before and 6-months after balloon pulmonary angioplasty (BPA), were retrospectively included. Ventricular blood pool values were measured, compared to control subjects (mean age 40.5 years ± 12.8 SD; 16 female) and correlated to invasive measures (CI, mPAP, PVR). Results: In both, control subjects and CTEPH patients, RVT1 and RVT2 were significantly reduced compared to LVT1 and LVT2. Compared to control subjects, RVT2 was significantly reduced in CTEPH patients (p = 0.0065) and increased significantly after BPA (p = 0.0048). Moreover, RVT2 was positively correlated with CI and negatively correlated with mPAP and PVR before (r = 0.5155, r = −0.2541, r = −0.4571) and after BPA (r = 0.4769, r = −0.2585, r = −0.4396). Conclusion: Ventricular blood pool T2 mapping might be novel non-invasive CMR imaging marker for assessment of disease severity, prognosis, follow-up and even therapy monitoring in PH.
Does Anxiety Affect Survival in Patients with Coronary Heart Disease?
Authors: Deter Hans-Christian , Albert Wolfgang , Weber Cora , Merswolken Melanie , Orth-Gomér Kristina , Herrmann-Lingen Christoph , Grün Anna-Sophia
Published: March 2023

Introduction: Behavioral and physiological risk factors worsen the prognosis of coronary heart disease (CHD). Anxiety is known to be a psychological predictor of CHD. In this study, we investigated whether this factor is associated with all-cause mortality in CHD patients in the long term. Methods: We studied 180 patients (mean age 60.6 SD 9.2 years, 26% women) with CHD from the Berlin Anxiety Trial (BAT) and the Stepwise Psychotherapy Intervention for Reducing Risk in Coronary Artery Disease (SPIRR-CAD) study. Their cardiac and psychological risk profile was represented by standardized procedures, including the Hospital Anxiety and Depression Scale (HADS) questionnaire. Mortality outcomes were assessed using a community-based registry. Results: Of 180 patients, we obtained information on all-cause mortality in 175 (96.7%) after a mean follow-up of 12.2 years (range 10.4–16.6 years). Of all participants, 54.4% had prior myocardial infarction, 95.3% had percutaneous transluminal coronary angioplasty and 22.2% had prior coronary artery bypass graft. Most of the patients (98.4%) had New York Heart Association class I and II, 25.6% had diabetes and 38.2% were smokers. Patients had a mean HADS anxiety score of 9.7 SD 4.1 at study entrance. We found the highest HADS anxiety quartile all-cause mortality in 14%, 30.2% in the middle quartiles and 58.7% in the lowest quartile (chi2 20.8, p = 0.001). Related to psychological mechanisms, a low level of anxiety, seemed to be a significant predictor of all-cause mortality. We found no advantage for patients who had received psychosocial therapy in terms of survival. Conclusion: These first data confirmed our hypothesis about the association of psychological risk factors with the long-term outcome of CAD patients. Future studies will clarify whether the severity of disease, age or a particular type of coping or denial mechanism are associated with the presented outcome in low-anxious patients.
Blood Cells Count Derived Inflammation Indexes as Predictors of Early Treatment Response to Dupilumab in Patients with Moderate-to-Severe Atopic Dermatitis
Authors: Zinellu Angelo , Sucato Federica , Piras Viviana , Addis Gian Mario , Biondi Gabriele , Montesu Maria Antonia , Mangoni Arduino A. , Carru Ciriaco , Pirina Pietro , Paliogiannis Panagiotis , Fois Alessandro G. , Satta Rosanna
Published: March 2023

Derived inflammatory indexes from routine hematological parameters might be useful for predicting early-response vs. late/non-response to dupilumab, the first biological agent approved for moderate-to-severe atopic dermatitis (AD). We tested this hypothesis by retrospectively investigating the association between pre-specified baseline inflammatory indexes and dupilumab response (≥50% reduction in the Eczema Area and Severity Index, EASI 50) at 4 and 16 weeks in a consecutive series of 66 AD patients (38 males and 28 females). Forty-six patients (69.7%) were early-responders at 4 weeks, whereas the remaining twenty (30.3%) were late/non-responders at 16 weeks. In logistic regression, the platelet-to-lymphocyte ratio (PLR) was independently associated with early-response (OR = 1.0159, 95% CI 1.0005 to 1.0315, p = 0.0426). The predictive performance of PLR and other derived indexes towards early-response was further improved by their combination with serum IgE concentrations, with a maximum AUC value for the combined systemic immune inflammation index (SII)-IgE of 0.797 (95% CI = 0.677 to 0.884, p < 0.0001). Derived inflammatory indexes, particularly SII-IgE, might be useful to identify early-responders to dupilumab and develop alternative treatment protocols for late/non-responders.
A Glider View of the Spreading and Mixing Processes of Antarctic Intermediate Water in the Northeastern Subtropical Atlantic
Authors: Jiménez-Rincón Juan Alberto , Cianca Andrés , Ferrero-Martín Carmen , Izquierdo Alfredo
Published: March 2023

The Antarctic Intermediate Water (AAIW), one of the most important global intermediate water masses, spreads over the world ocean. Its propagation limit at the Northeast Subtropical Atlantic is characterized by its encounter with the Mediterranean Water (MW), which presents dissimilar thermohaline properties. Previous studies of the AAIW in this region have been based on traditional oceanographic cruise observations, which were later complemented by observations using autonomous systems such as ARGO floats. However, these observations present limitations for the study of processes occurring at mesoscale and smaller scales. In this study, we used high-resolution observations made by cutting edge platforms such as underwater gliders. Specifically, a meridional glider section realized in spring 2016 between the islands of Madeira and Gran Canaria has been used. The temperature, salinity and dissolved oxygen minima have allowed the detection of the AAIW signal north of the Canarian archipelago and significantly westward from its main northward propagation pathway in this region. The results of this work have shown that the encounter of AAIW and MW generates thermohaline intrusions or interleaving layers. It is suggested that double diffusion processes may play a role in the development of these structures, which may be important for water masses mixing and, therefore, in determining the northward spreading boundary of AAIW. The use of the high-resolution glider observations combined with other data products is essential for the study of water masses and dynamics when relevant processes have a wide range of scales.
Development of Multiple Nucleotide Polymorphism Molecular Markers for Enoki Mushroom (Flammulina filiformis) Cultivars Identification
Authors: Liu Fei , Wang Shi-Hui , Jia Ding-Hong , Tan Hao , Wang Bo , Zhao Rui-Lin
Published: March 2023

The enoki mushroom (Flammulina filiformis) is one of the most important and popular edible mushrooms commercially in China. However, traditional mushroom cultivar identification is challenging due to poor accuracy, heavy workloads, and low reproducibility. To overcome this challenge, we developed a method for identifying F. filiformis strains using multiple nucleotide polymorphism sequencing (MNP-seq). This involved screening 179 universal MNP markers based on whole-genome sequencing data, constructing an MNP sequence library, and performing multiplex PCR amplification and high-sequencing. We further screened 69 core MNP markers and used them to build a neighbor-joining (NJ) phylogenetic tree of 232 cultivated and wild strains. Our analysis showed that all cultivars could be accurately separated by computing genetic similarity values and that the cultivars could be separated into 22 distinct evolutionary pedigrees. The specific value of genetic similarity can be used as the standard to distinguish F. filiformis cultivars, however, it needs to be comprehensively defined by the additional phenotype and biological characteristics of those strains in the future work.
Endophytic Fungi Regulate HbNHX1 Expression and Ion Balance in Hordeum bogdanii under Alkaline Stress
Authors: Long Feng , Hu Meng-Fei , Chen Sheng , Bao Gen-Sheng , Dan Han , Chen Shui-Hong
Published: March 2023

Plants cope with abiotic stress in several ways, including by collaborating with microorganisms. Epichloë, an endophytic fungus, has been shown to improve plant tolerance to extreme external environments. Hordeum bogdanii is a known salt-tolerant plant with the potential to improve alkaline lands. NHX1 plays a key role in the transport of ions in the cell and is overexpressed in plants with increased salt tolerance. However, the expression levels of HbNHX1 in Epichloë endophytic fungal symbionts in H. bogdanii have not been elucidated. We used Hordeum bogdanii (E+) with the endophytic fungi Epichloë bromicola and H. bogdanii (E−) without the endophytic fungi and compared the differences in the ion content and HbNHX1 expression between the shoots and roots of E+ and E− plants under alkaline stress. The absorption capacity of both K+ and Na+ of H. bogdanii with endophytic fungi was higher than that without endophytic fungi. In the absence of alkaline stress, endophytic fungi significantly reduced the Cl− content in the host H. bogdanii. Alkaline stress reduced SO42− content in H. bogdanii; however, compared with E−, endophytic fungi increased the content of SO42− in E+ plants. With an increase in the alkaline concentration, the expression of HbNHX1 in the roots of H. bogdanii with endophytic fungus exhibited an upward trend, whereas the expression in the shoots exhibited a downward trend first and then an upward trend. Under 100 mmol·L−1 mixed alkaline stress, the expression of HbNHX1 in E+ was significantly higher than that in E−, indicating that endophytic fungi could increase the Na+ region in vacuoles. The external environment affects the regulation of endophytic fungi in H. bogdanii and that endophytic fungi can play a key role in soil salinization. Therefore, the findings of this study will provide technical support and a theoretical basis for better utilization of endophytic fungi from H. bogdanii in saline land improvement.
Contribution of Morphology of Frontal Sinuses (Linear and Volumetric Measurements) to Gender Identification Based on Cone Beam Computed Tomography Images (CBCT): A Systematic Review
Authors: Mitsea Anastasia , Christoloukas Nikolaos , Rontogianni Aliki , Angelopoulos Christos
Published: March 2023

Human identification is considered as an important step in the reconstruction of biological profiles, especially of unknown individuals. Frontal sinuses (FS) have been regarded as an ideal anatomical structure for individualisation because few pathological conditions can potentially alter their shape. Aim: The aim of this review was to evaluate scientific evidence published since January 2010 and determine whether the dimensions and volume of FS might be useful parameters for gender determination and human identification, based only on cone beam computed tomography images (CBCT). Methods: This review was performed in accordance with the PRISMA statement. Four databases were searched for articles published between January 2010 and June 2022. Results: A total of 195 records were initially identified, and 90 remained after a manual duplicate check. Eight articles were selected for a full review according to the inclusion and exclusion criteria after title and abstract screening. A total of 718 participants (359 males and 359 females) were identified from the included studies. Frontal sinus volume (FSV) was significantly higher in male individuals. Frontal sinus height (FSH) and volume were the superior discriminating parameters for forensic identification. Conclusions: This review demonstrates that assessment of FS based on CBCT images could be beneficial for gender identification in forensic science. According to the obtained studies, frontal sinus volume (FSV) and frontal sinus height (FSH) are significant greater in males than in females, providing an additional complementary method. Larger sample size and common measurement protocols are needed to verify their usefulness.
A Systematic Review of Intermittent Theta Burst Stimulation for Neurocognitive Dysfunction in Older Adults with Schizophrenia
Authors: Zhang Xinyang , Yang Xinhu , Shi Zhanming , Xu Rui , Tan Jianqiang , Yang Jianwen , Huang Xiong , Huang Xingbing , Zheng Wei
Published: March 2023

Objective: Neurocognitive dysfunction is thought to be one of the core clinical features of schizophrenia, and older adults with schizophrenia exhibited greater overall cognitive deficits than younger adults. The aim of this systematic review was to examine the neurocognitive effects of intermittent theta burst stimulation (iTBS) as an adjunctive treatment for older adults suffering from schizophrenia. Methods: Randomized double-blinded controlled trials (RCTs) investigating the neurocognitive effects of adjunctive active iTBS versus sham iTBS in older adults with schizophrenia were systematically identified by independent investigators searching Chinese and English databases. Results: Two double-blinded RCTs (n = 132) compared the neurocognitive effects of adjunctive active iTBS (n = 66) versus sham iTBS (n = 66) in patients that fulfilled the inclusion criteria of this systematic review and were analyzed. One RCT found significant superiority of active iTBS over sham iTBS in improving neurocognitive performance in older adults with schizophrenia. In the other RCT, the findings on the neurocognitive effects of iTBS as measured by three different measurement tools were inconsistent. The dropout rate was reported in the two RCTs, ranging from 3.8% (3/80) to 7.7% (4/52). Conclusion: There is preliminary evidence that adjunctive iTBS may have some beneficial effects in the treatment of neurocognitive function in older patients with schizophrenia. Future RCTs with larger sample sizes focusing on the neurocognitive effects of adjunctive iTBS in older adults with schizophrenia are warranted to verify these findings.
Clinical Presentation Is Dependent on Age and Calendar Year of Diagnosis in Celiac Disease: A Hungarian Cross-Sectional Study
Authors: Szakács Zsolt , Farkas Nelli , Nagy Enikő , Bencs Réka , Vereczkei Zsófia , Bajor Judit
Published: March 2023

International trends indicate that celiac disease (CeD) is becoming more common, while the clinical presentation of CeD tends to change. We aimed to investigate factors associated with the clinical presentation of CeD. We reviewed all CeD cases diagnosed at our tertiary center, University of Pécs (Hungary), between 1992 and 2019. We collected data of verified CeD patients on clinical presentations (classified by the Oslo Classification), the age at and calendar year of diagnosis, and sex, serology and histology at diagnosis. To assess the associations of baseline variables with clinical presentations, we applied univariate and multivariate (binary logistic regression) statistics. A total of 738 CeD patients were eligible for inclusion. In the univariate analysis, patients with classical CeD were more common in the latest calendar period (p < 0.001) and tended to be older (p = 0.056), but we failed to observe a significant association between the clinical presentation and sex, serology or histology at diagnosis. In the multivariate analysis, only age at diagnosis and calendar year were independently associated with clinical presentations (OR = 1.02, CI: 1.01–1.04 and OR = 0.93, CI: 0.89–0.98, respectively). Our findings confirmed that classical CeD is independently associated with age at diagnosis and calendar year of diagnosis of CeD, whereas other parameters were not significantly associated with clinical presentations.
Camouflaged Compensation: Do South African Executives Increase Their Pay through Share Repurchases?
Authors: Steenkamp Gretha , Wesson Nicolene , Smit Eon v. d. M.
Published: March 2023

Increasingly, researchers in developed economies are associating the exponential growth in share repurchases with executives’ desire to increase company share price and thus the value of their own share-based compensation. As research on this topic in emerging economies is sparse, this paper investigates the relationship between share repurchases and executive share-based compensation in South Africa. Certain weaknesses in South African corporate governance relating to share repurchases exacerbate the risk of camouflaged rent extraction and unethical behaviour. Regression analyses were executed, using data on share repurchases and executive share-based compensation variables for listed South African companies for the period 2002–2017. Statistically significant positive relationships were identified between share repurchases and executive share-based compensation. The results support the proposition that South African executives may be repurchasing shares in a bid to increase the value of their share-based compensation (in line with the managerial power theory), rather than maximising long-term shareholder value. This paper emphasises the need for improved corporate governance relating to share repurchases in South Africa. Given the income inequality in South Africa, the findings also have social justice implications.
When Institutional Plates Collide: The Dynamic Impact of Informal Institutions on Capital Market Development
Authors: Lindorfer Robert , d’Arcy Anne , Filatotchev Igor
Published: March 2023

We provide an institutional theory perspective to examine societal legitimacy in the context of capital market development. While prior research has focused on the importance of formal institutions, firms are embedded within broader socio-economic structures associated with informal institutions. Using content analysis and a unique dataset of 3244 newspaper articles between 2004 and 2013, we develop a dynamic measure capturing the public perception of capital markets as a proxy of informal institutions. We run a Prais–Winsten regression with panel-corrected standard errors to explore the dynamic relationship between public perception of capital markets and equity market size in Austria and Poland. We further theoretically and empirically explore how formal and informal institutions mutually reinforce each other in the context of capital market development. Our results suggest that informal institutions matter differently in developed and emerging economies.
Case Study: Impact of Regulatory Restrictions and Tax Policy on Breakeven Analysis and Risk Management
Authors: Dunne James Henry , Harris Peter , Kinkela Katherine
Published: March 2023

The objective of this case study is to enable students to analyze the financial impact of an unexpected catastrophic event on a retail business and how the strategic operational decisions made in response to regulatory restrictions and changes in tax policy impact the business’s risk tolerance and breakeven analysis. To provide students with a context for comparison, this case study provides students with the opportunity to analyze the financial statements of a retail business prior to the occurrence of an unexpected catastrophic event, how the catastrophic event impacted revenue and profitability, and how the risk reduction strategies the business employed contained the adverse impact of the factors brought on by that catastrophic event on breakeven. This case study addresses a core gap in the body of knowledge by analyzing a business in three distinct stages of the business life cycle: (1) in the start-up phase; (2) in pre-crisis operations mode; and (3) in crisis mode confronted with an unexpected catastrophic event amidst governmental shutdowns, state and federal regulatory restrictions, and emergency changes to the tax policy. Examining a fictitious restaurant (a composite of the sales statistics of three actual restaurants located in Long Island, New York from 2019 to 2021) in operation for one year prior to the COVID-19 pandemic, students are given the opportunity to think critically about how strategic operational decisions made to generate sales, to minimize risk, to comply with mandated state government policy, and to take advantage of federal tax relief policy, collectively changed the financial projections and impacted the breakeven analysis of that business. Students are able to evaluate business start-up costs, first year (pre-pandemic) sales and costs, and second year (during pandemic) sales and costs of a retail business. Students then evaluate how the United States’ federal PPP relief loan program, along with other pandemic relief programs for businesses and individuals, impacted profitability and business strategy. Students also assess risk, risk tolerance, and how the strategies employed to minimize risk impact a business. The motivation for this case study is to provide students with an illustrative example of an entity at various stages of the business life cycle, to explore the surrounding context of the impact of external environmental events, and to identify the effects of strategic operational responses to the various regulatory changes that were brought on by a catastrophic event. This case study is designed for use in courses that study revenue projection, tax, internal controls, breakeven analysis, and risk management. Teaching Note: While this case study uses a restaurant as a model, prior understanding of the restaurant industry is not necessary. Any student or instructor can use their practical knowledge and experience as a consumer to adequately analyze the issues presented.
Revealing the Correlation between Molecular Structure and Corrosion Inhibition Characteristics of N-Heterocycles in Terms of Substituent Groups
Authors: Tan Li , Li Jiusheng , Zeng Xiangqiong
Published: March 2023

Controlling metal corrosion can directly address the waste of metal and the environmental pollution and resource depletion caused by metal recycling, very significant factors for green and sustainable development. The addition of corrosion inhibitors is a relatively cost-effective means of corrosion prevention. Among these, N-heterocycles have been widely used because heteroatoms contain lone pairs of electrons that can be strongly adsorbed onto metals, protecting them in highly corrosive environments at relatively low concentrations. However, due to the large variety of N-heterocycles, their corrosion inhibition characteristics have seldom been compared; therefore, the selection of appropriate N-heterocycles in the development of anti-corrosion products for specific applications was very difficult. This review systematically analyzed the influence of different substituents on the corrosion inhibition performance of N-heterocycles, including different alkyl chain substituents, electron-donating and electron-withdrawing substituents, and halogen atoms, respectively. The correlation between the molecular structure and corrosion inhibition characteristics of N-heterocycles was comprehensively revealed, and their action mechanism was analyzed deeply. In addition, the toxicity and biodegradability of N-heterocycles was briefly discussed. This study has provided a significant guideline for the development of green, promising corrosion inhibitors for advanced manufacturing and clean energy equipment protection.
Accumulation of Plastic Strain at Notch Root of Steel Specimens Undergoing Asymmetric Fatigue Cycles: Analysis and Simulation
Authors: Hatami Faezeh , Varvani-Farahani Ahmad
Published: March 2023

The present study evaluates the ratcheting response at notch roots of 1045 steel specimens experiencing uniaxial asymmetric fatigue cycles. Local stress and strain components at the notch root were analytically evaluated through the use of Neuber, Glinka, and Hoffman-Seeger (H-S) rules coupled with the Ahmadzadeh-Varvani (A-V) kinematic hardening model. Backstress promotion through coupled kinematic hardening model with the Hoffman-Seeger, Neuber, and Glinka rules was studied. Relaxation in local stresses on the notched samples as hysteresis loops moved forward with plastic strain accumulation during asymmetric loading cycles was observed. Local ratcheting results were simulated through FE analysis, where the Chaboche model was employed as the materials hardening rule. A consistent response of the ratcheting values was evidenced as predicted, and simulated results were compared with the measured ratcheting data.
Effect of Gd3+, La3+, Lu3+ Co-Doping on the Morphology and Luminescent Properties of NaYF4:Sm3+ Phosphors
Authors: Nosov Viktor G. , Betina Anna A. , Bulatova Tatyana S. , Guseva Polina B. , Kolesnikov Ilya E. , Orlov Sergey N. , Panov Maxim S. , Ryazantsev Mikhail N. , Bogachev Nikita A. , Skripkin Mikhail Yu , Mereshchenko Andrey S.
Published: March 2023

The series of luminescent NaYF4:Sm3+ nano- and microcrystalline materials co-doped by La3+, Gd3+, and Lu3+ ions were synthesized by hydrothermal method using rare earth chlorides as the precursors and citric acid as a stabilizing agent. The phase composition of synthesized compounds was studied by PXRD. All synthesized materials except ones with high La3+ content (where LaF3 is formed) have a β-NaYF4 crystalline phase. SEM images demonstrate that all particles have shape of hexagonal prisms. The type and content of doping REE significantly effect on the particle size. Upon 400 nm excitation, phosphors exhibit distinct emission peaks in visible part of the spectrum attributed to 4G5/2→6HJ transitions (J = 5/2–11/2) of Sm3+ ion. Increasing the samarium (III) content results in concentration quenching by dipole–dipole interactions, the optimum Sm3+concentration is found to be of 2%. Co-doping by non-luminescent La3+, Gd3+ and Lu3+ ions leads to an increase in emission intensity. This effect was explained from the Sm3+ local symmetry point of view.