bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation of Concentration and Distribution of Elements in Three Environmental Compartments in the Region of Mitrovica, Kosovo: Soil, Honey and Bee Pollen
Authors: Kastrati Granit , Paçarizi Musaj , Sopaj Flamur , Tašev Krste , Stafilov Trajče , Mustafa Mihone Kerolli
Published: February 2021

The abundances of selected elements in different environmental compartments, namely soil, honey, and bee pollen, was determined in this study. For that purpose, sixteen soil and honey samples, and nine pollen samples were taken in the region of Mitrovica, Kosovo. The concentration of elements was measured by ICP-AES and ICP-MS. Pollution level concentrations of Pb, Zn, As, and Cd were observed in soil. The level of soil pollution was estimated by calculating pollution indices. Pb was also observed at high concentrations in honey, as was Cd and Pb in pollen. Pearson’s correlation coefficients revealed mostly weak and moderate correlations of the concentrations of the eight selected elements among the soil, honey, and pollen samples. Several groups of elements with geogenic and anthropogenic origin were identified by hierarchical cluster analysis. The concentrations of selected heavy metals for soil and honey were compared to those in neighboring countries, and those for pollen with samples from Turkey, Serbia, and Jordan.
Patulin Contamination of Citrus Fruits from Punjab and Northern Pakistan and Estimation of Associated Dietary Intake
Authors: Aslam Kinza , Iqbal Shahzad Zafar , Razis Ahmad Faizal Abdull , Usman Sunusi , Ali Nada Basheir
Published: February 2021

This research aims to assess the natural occurrence of patulin (PAT) in selected citrus fruits from central cities of Punjab and Pakistan’s northern cities. A total of 2970 fruit samples from 12 citrus cultivars were examined using liquid chromatography fitted with a UV detector. The detection limit (LOD) and quantification limit were 0.04 and 0.12 µg/kg, respectively. About 56% of samples of citrus fruits from Punjab’s central cities, Pakistan, were found to be contaminated with PAT, with values ranging from 0.12 to 1150 µg/kg in samples from central Punjab cities. Furthermore, 31.7% of samples of citrus fruits from northern cities of Pakistan were contaminated with PAT, with values ranging from 0.12 to 320 µg/kg. About 22.1% of citrus fruit samples had PAT levels greater than the suggested limits established by the European Union (EU). The dietary intake levels of PAT ranged from 0.10 to 1.11 µg/kg bw/day in the central cities of Punjab, Pakistan, and 0.13 to 1.93 µg/kg bw/day in the northern cities of Pakistan.
The Effects of Psychological Skills Training for Archery Players in Korea: Research Synthesis Using Meta-Analysis
Authors: Kim Eui Jae , Kang Hyun Wook , Park Seong Man
Published: February 2021

The purpose of this meta-analysis study was to investigate the effectiveness of psychological skills training interventions for archery players in Korea. A total of 17 research papers examining the effects of psychological skills training interventions were selected for data analysis. To analyze the data, the Comprehensive Meta-Analysis (CMA) software program was used. The results of this study were as follows: The overall effect size of psychological skills training interventions for archery players was ES = 0.469 (Hedges’ g), which was a small effect size. The major factors that influenced the effects of psychological skills training interventions for archery players appeared to be the player level and training period. In particular, adult players and longer periods of training had bigger effect sizes. The results of this study demonstrate that psychological skills training for archery players is effective and show that the player level and training period are crucial factors in the psychological skills training design. Based on these findings, some implications for future research are discussed.
Effects of Short-Term Plyometric Training on Agility, Jump and Repeated Sprint Performance in Female Soccer Players
Authors: Maciejczyk Marcin , Błyszczuk Renata , Drwal Aleksander , Nowak Beata , Strzała Marek
Published: February 2021

The aim of the study was to determine the effects of short-term (4 weeks, twice a week: 8 sessions) plyometric training on agility, jump, and repeated sprint performance in female soccer players. The study comprised 17 females performing this sports discipline. The players were randomly divided into two groups: with plyometric training (PLY) and the control (CON). All players followed the same training program, but the PLY group also performed plyometric exercises. Tests used to evaluate physical performance were carried out immediately before and after PLY. After implementing the short PLY training, significant improvement in jump performance (squat jump: p = 0.04, ES = 0.48, countermovement jump: p = 0.009, ES = 0.42) and agility (p = 0.003, ES = 0.7) was noted in the PLY group. In the CON group, no significant (p > 0.05) changes in physical performance were observed. In contrast, PLY did not improve repeated sprint performance (p > 0.05) among female soccer players. In our research, it was shown that PLY can also be effective when performed for only 4 weeks instead of the 6–12 weeks typically applied.
Attachment Representation and Emotion Recognition Ability in Children with ADHD and Their Parents: A Study Protocol
Authors: Kissgen Ruediger , Franke Sebastian , Susewind Moritz , Krischer Maya
Published: February 2021

Background: Few studies in clinical attachment research to date have examined children with an attention-deficit/hyperactivity disorder (ADHD) diagnosis. This is surprising for two reasons: first, there are a number of parallels between the behaviors of children with an insecure and disorganized attachment and the behaviors of children with an ADHD diagnosis. Second, secure attachment has a positive effect on the development of skills in areas in which children with ADHD demonstrate problems (e.g., attention span, impulse control). There are currently no findings on whether or not and how insecure and disorganized attachment and ADHD affect children’s emotion recognition ability. Methods: This is a cross-sectional study, part exploratory and part hypothesis-driven in the context of basic research. A clinical sample of 5- to 10-year-old children with an ADHD diagnosis and their parents is to be compared to a non-clinical unaffected control group. Over a period of 3 years, 80 subjects and their parents are to be recruited in each group for participation in the study. Discussion: This study is the first to examine links between attachment, emotion recognition ability, and ADHD. It is also the first to include not just children with ADHD but also their mothers and fathers in its design. The findings should help reduce the research gap and generate more knowledge for family interventions in the case of ADHD.
Perceived Barriers to COVID-19 Testing
Authors: McElfish Pearl A. , Purvis Rachel , James Laura P. , Willis Don E. , Andersen Jennifer A.
Published: February 2021

(1) Background: Prior studies have documented that access to testing has not been equitable across all communities in the US, with less testing availability and lower testing rates documented in rural counties and lower income communities. However, there is limited understanding of the perceived barriers to coronavirus disease 2019 (COVID-19) testing. The purpose of this study was to document the perceived barriers to COVID-19 testing. (2) Methods: Arkansas residents were recruited using a volunteer research participant registry. Participants were asked an open-ended question regarding their perceived barriers to testing. A qualitative descriptive analytical approach was used. (3) Results: Overall, 1221 people responded to the open-ended question. The primary barriers to testing described by participants were confusion and uncertainty regarding testing guidelines and where to go for testing, lack of accessible testing locations, perceptions that the nasal swab method was too painful, and long wait times for testing results. (4) Conclusions: This study documents participant reported barriers to COVID-19 testing. Through the use of a qualitative descriptive method, participants were able to discuss their concerns in their own words. This work provides important insights that can help public health leaders and healthcare providers with understanding and mitigating barriers to COVID-19 testing.
Factors Associated with Poor Quality of Sleep in Construction Workers: A Secondary Data Analysis
Authors: Kim Youkyung , Lee Sangeun , Lim Jeeyeon , Park Soyeon , Seong Sojeong , Cho Youngshin , Kim Heejung
Published: February 2021

This study aimed to explore factors associated with poor quality of sleep in construction workers. This study was cross-sectional, correlational in design and used secondary data from fatigue instrument development study. We analyzed the data from 206 participants aged over 19 years who worked at construction sites for more than 6 months. We used multivariate binary logistic regression to identify the factors associated with poor quality of sleep. We classified the two sleep quality groups based on the Pittsburgh Sleep Quality Index (PSQI) score, and almost 63% of them were classified as the poor quality of sleep group. Based on multivariate binary logistic regression (Cox and Snell R2 = 0.317, Nagelkerke R2 = 0.429), the poor quality of sleep group tended to sleep for a shorter duration before the working day, and not only showed lower sleep latency and higher levels of daytime dysfunction and discomfort in daily life, but also had more chronic disease, depressive symptoms, and higher physical fatigue. Our study findings support that there are many modifiable factors associated with poor sleep and a high rate of poor quality of sleep occurred in construction workers. Thus, clinicians should consider providing diverse options for applying interventions to ensure better sleep, fatigue management, and depression prevention in construction workers after considering their unique characteristics.
The Israel National Sera Bank: Methods, Representativeness, and Challenges
Authors: Bassal Ravit , Cohen Dani , Green Manfred S. , Keinan-Boker Lital
Published: February 2021

The Israel National Sera Bank (INSB) was established in 1997 in the Israel Center for Disease Control. The purpose of the INSB was to provide policymakers with data on the immunity status of the Israeli population against vaccine-preventable diseases, and on the extent and characteristics of exposure to emerging and re-emerging infectious diseases. The aim of this paper is to describe the methods, representativeness, and challenges in maintaining the INSB. The INSB comprises residual sera collected in six laboratories. By the end of 2019, 138,898 samples had been deposited in the INSB. These include samples from four community laboratories: 30.7% from the National Blood Service, 22.2% from Haifa and the Western Galilee, 21.7% from Soroka, and 0.7% from Jerusalem; and from two medical center laboratories: 18.6% from Schneider and 6.1% from Mayanei Hayeshua. The demographic characteristics of the sample at the end of 2019 closely resembled those of the general population. The main challenges addressed in maintaining the INSB relate to its representativeness, the possibility of repeated donors, costs, stability of antibody levels after long-term storage, ethical aspects, and the data available for each sample. The INSB is a unique, powerful, and necessary tool for assessing population immunity levels, based on serum samples collected over a long period of time.
Change in Denture Procedures after Implementation of National Health Insurance Coverage for the Elderly in Korea: A Nationwide Database
Authors: Lee Ye Seol , Kim Juyeong , Choy Yoon Soo , Kim Eunkyong , Yoo Jaehyun
Published: February 2021

Background: Dental health is an important factor in daily life routines and is closely associated with maintaining a health-related quality of life. This study examined denture procedure changes after implementation of the National Health Insurance (NHI) Coverage of Dentures for the elderly. Methods: We used the “Korean Community Health Survey (KCHS)” developed by the Korea Centers for Diseases Control and Prevention. We analyzed the association between policy implementation and dental health-related outcomes using difference-in-differences (DID) analysis to compare patients aged ≥75 with those 65–74 years before and after coverage. Results: A comparison of age groups and coverage periods showed that patients aged ≥75 years had higher (OR: 1.038, 95% CI: 1.021–1.055) procedure rates after coverage. In particular, elderly patients on medical aid had significantly higher denture procedure rates, while those without oral health screening were more likely to have denture procedures. Conclusions: This study determined the impact of the NHI Coverage of Denture procedure policy for the elderly and found increased denture treatments in the elderly. This policy appeared to positively affect older patients by increasing denture procedures for low-income and medical aid beneficiaries. Hence, the government needs to increase oral health examination and dental health policies for the elderly.
Chaos Analysis of Urban Low-Carbon Traffic Based on Game Theory
Authors: Wu Xiaohui , He Ren , He Meiling
Published: February 2021

Developing urban low-carbon traffic is an effective measure to reduce traffic carbon emissions, which are important parts of greenhouse gas. In order to understand the development characteristics and regular patterns of urban low-carbon traffic, we present a game model that enables us to predict the possible range of travel mode choice and the impact of low-carbon awareness. Through chaos analysis and simulation of the model, the authors come to realize that the proportions of travel mode choice can reach an equilibrium under a certain urban traffic system. This equilibrium is related to low-carbon awareness and the situation of the urban traffic system. The research we have done suggests that in small cities with undeveloped traffic systems, the most effective measure to achieve urban low-carbon traffic is to increase the comprehensive costs of high-carbon travel. However, in big cities with developed traffic systems, raising low-carbon awareness of residents can greatly increase the proportion of low-carbon travelers and improve the stability of travel mode choice. The results could provide development strategies and policy suggestions for urban low-carbon traffic and reduce the adverse impact of urban traffic emissions on public health.
The Synergistic Process of Improvement in Cognitive Behavioral Therapy for Major Depression
Authors: Malkomsen Anders , Røssberg Jan Ivar , Dammen Toril , Wilberg Theresa , Løvgren André , Horgen Evensen Julie
Published: February 2021

Background: There is a substantial lack of qualitative research concerning individual cognitive behavioral therapy (CBT) for patients with major depressive disorder (MDD). In the present study, we wanted to explore how patients suffering from MDD experience improvement in CBT. Method: Patients with MDD (N = 10) were interviewed at therapy termination with semi-structured qualitative interviews. The transcripts were analyzed using a thematic analysis approach. Results: We identified three elements that were relevant to the process of improvement for all patients: the therapeutic relationship, the therapeutic interventions and increased insight. There is a dynamic interrelationship and synergy between these elements that may explain why patients considered the same elements as helpful, but often in different ways and at different stages of therapy. Conclusions: Highlighting the synergies and interrelationship between the elements that patients experience as helpful, may help therapists to learn from and utilize these experiences. This is a reminder of the importance of always being attentive to the individual processes of patients.
The Associations between Physical Fitness, Complex vs Simple Movement, and Academic Achievement in a Cohort of Fourth Graders
Authors: Ryu Jong-Sik , Chung Hae Ryong , Meador Benjamin M. , Seo Yongsuk , Kim Kyung-O
Published: February 2021

This study analyzed the correlation between elementary school students’ body composition, physical activity, physical fitness, movement ability, and academic achievement. Movements ranged from simple actions to complex movements requiring executive functioning. In total, 110 fourth graders (60 boys, 50 girls) participated in this experiment. Body composition (BMI, % of body fat), physical activity (pedometer), physical fitness (muscular strength, endurance, power, flexibility, and VO2max), and complex movement abilities (Illinois Agility test, soda pop hand test, and soda pop foot test) were measured. Regression modeling of body composition and fitness/activity variables was able to account for 30.5% of the variation of total academic scores in females, but only 4.3% in males. No individual tests were reliably correlated with multiple academic outcomes in males. However, hand and foot soda pop times, as well as Illinois Agility scores, were repeatedly correlated with academic outcomes in females, each correlating with 4 of the 6 academic scores. Body composition and physical activity level did not correlate with academic achievement, and simple physical fitness showed a low correlation with academic achievement in both boys and girls. On the other hand, complex, cognitively demanding movements such as the Illinois Agility, soda pop hand, and soda pop foot tests had consistent correlations with academic achievement in girls, but not in boys.
Source Identification of Heavy Metals in Surface Paddy Soils Using Accumulated Elemental Ratios Coupled with MLR
Authors: Ma Jie , Chen Yali , Weng Liping , Peng Hao , Liao Zhongbin , Li Yongtao
Published: February 2021

Source identification of heavy metals in agricultural soils using small sample sizes, simple experimental procedures, and convenient analysis is urgently required. This study employed a simple source identification model using a visual comparison via radar plots, cluster analysis, principal component analysis, and a multiple linear regression model to determine the source of heavy metal pollution in soil samples from the Chang-Zhu-Tan urban agglomeration area of China. The elemental compositions of major pollution sources (atmospheric deposition, organic fertilizer, irrigation water, and tailings) were compared with soil samples from 11 study locations and the model was used to determine the relative contribution of different pollution sources at each sample site. The results showed that the model successfully calculated the contribution of different pollution sources at each site based on the pollution characteristics and contaminant transport rules of the region. The proposed method overcomes the requirement for extensive data and complex experimental procedures. Furthermore, the model can determine the source of heavy metal contamination in single or small plots, which is important for the prevention and control of heavy metal soil pollution and remediation at the plot scale.
Health Impacts of Urban Bicycling in Mexico
Authors: Rojas-Rueda David
Published: February 2021

Background: Bicycling has been associated with health benefits. Local and national authorities have been promoting bicycling as a tool to improve public health and the environment. Mexico is one of the largest Latin American countries, with high levels of sedentarism and non-communicable diseases. No previous studies have estimated the health impacts of Mexico’s national bicycling scenarios. Aim: Quantify the health impacts of Mexico urban bicycling scenarios. Methodology: Quantitative Health Impact Assessment, estimating health risks and benefits of bicycling scenarios in 51,718,756 adult urban inhabitants in Mexico (between 20 and 64 years old). Five bike scenarios were created based on current bike trends in Mexico. The number of premature deaths (increased or reduced) was estimated in relation to physical activity, road traffic fatalities, and air pollution. Input data were collected from national publicly available data sources from transport, environment, health and population reports, and surveys, in addition to scientific literature. Results: We estimated that nine premature deaths are prevented each year among urban populations in Mexico on the current car-bike substitution and trip levels (1% of bike trips), with an annual health economic benefit of US $1,897,920. If Mexico achieves similar trip levels to those reported in The Netherlands (27% of bike trips), 217 premature deaths could be saved annually, with an economic impact of US $45,760,960. In all bicycling scenarios assessed in Mexico, physical activity’s health benefits outweighed the health risks related to traffic fatalities and air pollution exposure. Conclusion: The study found that bicycling promotion in Mexico would provide important health benefits. The benefits of physical activity outweigh the risk from traffic fatalities and air pollution exposure in bicyclists. At the national level, Mexico could consider using sustainable transport policies as a tool to promote public health. Specifically, the support of active transportation through bicycling and urban design improvements could encourage physical activity and its health co-benefits.
Community Pharmacy Practice in Italy during the COVID-19 (SARS-CoV-2) Pandemic: Regulatory Changes and a Cross-Sectional Analysis of Seroprevalence
Authors: Baratta Francesca , Visentin Giulio Mario , Ravetto Enri Lorenzo , Parente Marco , Pignata Irene , Venuti Francesco , Di Perri Giovanni , Brusa Paola
Published: February 2021

Pharmacists in the community and the essential requirement to safeguard their own health have become fundamental since the spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The aims of this paper were (I) to analyze the directives provided to pharmacists in 2020 regarding preventative safety measures to be adopted; (II) to determine the number of pharmacists who came into contact with SARS-CoV-2 in North-West Italy and relate this to the adopted preventative measures. The first aim was pursued by conducting a bibliographic research, consulting the principal regulatory sources. The second one was achieved with an observational study by administering a questionnaire and performing a serological test. The various protection measures imposed by national and regional legislation were analyzed. Two hundred and eighty-six pharmacists (about 8% of the invited ones) responded to the survey. Ten pharmacists reported a positive result to the serological test. Of the subjects who presented a positive result, three declared that they had not used a hand sanitizer, while two stated that they had not scheduled the cleaning and decontamination of surfaces. Two interviewees had not set up a system of quota restrictions on admissions. In four cases, a certified cleaning company had decontaminated the premises. The results of our study show that during the coronavirus disease 2019 (COVID-19) pandemic, the most pressing challenge for community pharmacists has been the protection of staff and clients inside the pharmacy; the challenge to be faced in the near future will probably be the management of new responsibilities.
Estimating the Economic Burden of Low Health Literacy in the Blacktown Community in Sydney, Australia: A Population-Based Study
Authors: Tannous Wadad Kathy , Ahmed Moin Uddin , John James Rufus , Reece Graham , Ahlenstiel Golo
Published: February 2021

Evidence shows that inadequate or low health literacy (LHL) levels are significantly associated with economic ramifications at the individual, employer, and health care system levels. Therefore, this study aims to estimate the economic burden of LHL among a culturally and linguistically diverse (CALD) community in Blacktown: a local government area (LGA) in Sydney, Australia. This study is a secondary analysis of cross-sectional data from publicly available datasets, including 2011 and 2016 census data and National Health Survey (NHS) data (2017–2018) from the Australian Bureau of Statistics (ABS), and figures on Disease Expenditure in Australia for 2015–2016 provided by the Australian Institute of Health and Welfare (AIHW). This study found that 20% of Blacktown residents reported low levels of active engagement with health care providers (Domain 6 of the Health Literacy Questionnaire (HLQ)), with 14% reporting a limited understanding of the health information required to take action towards improving health or making health care decisions (Domain 9 of the HLQ). The overall extra/delta cost (direct and indirect health care costs) associated with LHL in the Blacktown LGA was estimated to be between $11,785,528 and $15,432,239 in 2020. This is projected to increase to between $18,922,844 and $24,191,911 in 2030. Additionally, the extra disability-adjusted life year (DALY) value in 2020, for all chronic diseases and age-groups—comprising the extra costs incurred due to years of life lost (YLL) and years lived with disability (YLD)—was estimated at $414,231,335. The findings of our study may enable policymakers to have a deeper understanding of the economic burden of LHL in terms of its impact on the health care system and the production economy.
Health Investment Management and Healthcare Quality in the Public System: A Gender Perspective
Authors: Valls Martínez María del Carmen , Ramírez-Orellana Alicia , Grasso Mayra Soledad
Published: February 2021

The aim of this empirical research was to provide useful information for health system managers on the costs and investments involved in improving the quality of the National Health Service (NHS) based on patient assessments and from a gender perspective, i.e., without assuming that the perceived experience is identical for men and women. A cross-sectional study of 31 variables was applied using partial least squares structural equation modeling (PLS-SEM) as a research tool. The data were obtained from the Spanish Ministry of Health, Consumption, and Social Welfare for the entire Spanish territory between 2005 and 2018. The influence of expenditure, resource allocation, and mortality was hypothesized with regard to patient satisfaction according to disconfirmation theory. Patient satisfaction reflects clinical effectiveness, and therefore is a measure of health system quality. The results show that women are more sensitive to public investment in health than men, i.e., an increase in the level of spending and resources increases satisfaction more in women. In both sexes, the level of expenditure has a direct influence on patient satisfaction, and therefore on the quality of the healthcare system. It is important to increase spending on primary care, especially on specialized medical care and diagnostic equipment. However, reducing the use of drugs in favor of alternative treatments or therapies is considered to be positive. Likewise, spending has an impact on available resources, and these, in turn, have a positive influence on the level of use and a negative impact on mortality. Resources, especially healthcare staff, nuclear magnetic resonance equipment, and the number of posts in day hospitals, increase patients’ positive perception of the NHS.
Profiles of Working Moms’ Daily Time Use: Exploring Their Impact on Leisure
Authors: Kim Youngseo , Hong Sehee
Published: February 2021

This paper identified latent profiles depending on the patterns of daily time usage amongst working moms in Korea and tested their relations to family- and work-related characteristics. The consequent differences in the levels of leisure attributes were further investigated. Taking a holistic approach, latent profile analysis, one of the person-centered methods, was conducted using data drawn from the seventh year of the Korean Longitudinal Survey of Women and Families (KLoWF), with a sample of 1074 women. The results of this study indicate that three different subtypes of individuals emerged: a low-level care group (82.1%), a medium-level care group (13.8%), and a high-level care group (4.1%). The factors determining the classification for the profiles were the existence of preschool children, household income, gender role attitudes, and domestic help. Work-related factors proved to have no significant effect on time-use patterns. Profile membership was related to leisure attributes as perceived by working moms: the low-level care group reported the highest level of leisure time adequacy and leisure satisfaction, while the high-level care group presented the lowest level of sufficiency and satisfaction in their leisure time. Based on these findings, the article discusses the practical implications for enhancing the quality of life of working mothers.
Could Physical Activity Have any Role in Cardiovascular Disease Prevention in Prisoners? A Systematic Review
Authors: Papa Veronica , Tafuri Domenico , Vaccarezza Mauro
Published: February 2021

More than 10.74 million people are currently held in penal institutions worldwide. Moreover, there is also evidence that the percentage of elder and female prisoners has been consistently growing. Cardiovascular diseases are the leading cause of death worldwide. Exercise training and physical activity help to prevent both primary and secondary cardiovascular events. Data on the influence of physical activity on the well-being in prison population is scarce. Here, we discussed, in a systematic review, the general health conditions and the cardiovascular risk profile in the prisoners compared to the general population and evaluated whether or not exercise could be a valuable tool in preventing these diseases in inmates. We performed a systematic review following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement: 769 were initially identified, and a total of 24 studies were finally included. Nine studies evaluated the health conditions in prisoners, five studies evaluated the incidence of cardiovascular disease (CVD) and coronary heart disease (CHD) in the prison population, and 10 studies evaluated the feasibility and the effectiveness of exercise programs in prisoners. Sports-educational programs can benefit prison inmates. It appears that supervised exercise training is an effective coping strategy to deal with incarceration. Moreover, it seems the sports programs might be a useful tool in improving physical and mental health of prisoners as well as in decreasing cardiovascular risk factors.
The Knowledge and Attitude of Patients Diagnosed with Epithelial Ovarian Cancer towards Genetic Testing
Authors: Shin Wonkyo , Jeong Gowoon , Son Yedong , Seo Sang-Soo , Kang Sokbom , Park Sang-Yoon , Lim Myong Cheol
Published: February 2021

This study assessed the knowledge and attitude of patients with ovarian cancer (OC) toward OC and next generation sequencing (NGS). The data, including characteristics of patients, their knowledge about OC and their knowledge and attitude of NGS, were collected from June to October 2018. Of the 103 participants, 70.9% (n = 73) had cancer within the second-degree relatives, and 18.4% (n = 19) had BRCA pathogenic mutations. The percentage of right answer for the knowledge about OC and NGS was 64.7% (11/17) and 50% (6/12), respectively. The median number of patients who had positive expectations for the genetic test was 34 (range, 22–44). Based on a first-degree familial history, patients had a different degree of knowledge about OC (11 vs. 8.5, p = 0.026) and NGS (6.5 vs. 5, p = 0.011), but patients with a BRCA pathogenic mutation did not have a different degree of knowledge about OC and NGS panel testing. High-income families had a more positive attitude towards the genetic test than low-income families (p = 0.005). Women with OC do not have enough knowledge about OC (11/17, 64.7%) and NGS (6/12, 50%) but they showed a positive attitude toward the NGS test. These women need OC and NGS educational intervention.
Job Perceptions Contribute to Stress among Secondary School Teachers in Southwestern Uganda
Authors: Ssenyonga Joseph , Hecker Tobias
Published: February 2021

(1) Background: Teachers’ personal and strenuous working conditions reflect the realities of the teaching vocation that may result in increased stress levels and associated negative consequences, such as negative emotions. It is also well-known that teacher stress contributes to more violence against students. However, little is known about personal and school context factors that contribute to teachers’ stress. The current study examined whether, in addition to school-related factors, job perceptions, including the feeling of pressure at work and perceived school climate and teaching difficulties, contribute to teachers’ stress. (2) Methods: A representative sample of 291 teachers from 12 public secondary schools in southwestern Uganda responded to self-administered questionnaires. (3) Results: Teaching difficulties and feelings of pressure at work contributed to teachers’ stress. Furthermore, stress did not vary with teachers’ sociodemographic variables. (4) Conclusions: Teachers’ perceptions of their working conditions were associated with teacher stress levels. Therefore, more efforts need to be geared towards improving the working conditions of teachers as a way of reducing stress.
Transmission of Trading Orders through Communication Line with Relativistic Delay
Authors: Lerner Peter B.
Published: February 2021

The notion of “relativistic finance” became ingrained in the public imagination and has been asserted in many mass-media reports. However, despite an observed drive of the most reputable Wall Street firms to establish their servers ever closer to the trading hubs, there is surprisingly little concrete information related to the relativistic delay of the trading orders. There is an underlying assumption that faster electronics are always beneficial to the stability of the network. In this paper, the author proposes a modified M/M/G queue theory to describe the propagation of the trading signal with finite velocity. Based on this theory, we demonstrate that, even if the reaction time of the system is negligible, the propagating signal is distorted by simple acts of trading along the transmission line.
A Unified Methodology for the Generalisation of the Geometry of Features
Authors: Barańska Anna , Bac-Bronowicz Joanna , Dejniak Dorota , Lewiński Stanisław , Krawczyk Artur , Chrobak Tadeusz
Published: February 2021

The development of generalisation (simplification) methods for the geometry of features in digital cartography in most cases involves the improvement of existing algorithms without their validation with respect to the similarity of feature geometry before and after the process. It also consists of the assessment of results from the algorithms, i.e., characteristics that are indispensable for automatic generalisation. The preparation of a fully automatic generalisation for spatial data requires certain standards, as well as unique and verifiable algorithms for particular groups of features. This enables cartographers to draw features from these databases to be used directly on the maps. As a result, collected data and their generalised unique counterparts at various scales should constitute standardised sets, as well as their updating procedures. This paper proposes a solution which consists in contractive self-mapping (contractor for scale s = 1) that fulfils the assumptions of the Banach fixed-point theorem. The method of generalisation of feature geometry that uses the contractive self-mapping approach is well justified due to the fact that a single update of source data can be applied to all scales simultaneously. Feature data at every scale s < 1 are generalised through contractive mapping, which leads to a unique solution. Further generalisation of the feature is carried out on larger scale spatial data (not necessarily source data), which reduces the time and cost of the new elaboration. The main part of this article is the theoretical presentation of objectifying the complex process of the generalisation of the geometry of a feature. The use of the inherent characteristics of metric spaces, narrowing mappings, Lipschitz and Cauchy conditions, Salishchev measures, and Banach theorems ensure the uniqueness of the generalisation process. Their application to generalisation makes this process objective, as it ensures that there is a single solution for portraying the generalised features at each scale. The present study is dedicated to researchers concerned with the theory of cartography.
Effect of Selected Anionic and Cationic Drugs Affecting the Central Nervous System on Electrical Properties of Phosphatidylcholine Liposomes: Experiment and Theory
Authors: Kotyńska Joanna , Naumowicz Monika
Published: February 2021

Interactions between phospholipid membranes and selected drugs affecting the central nervous system (CNS) were investigated. Small, unilamellar liposomes were used as biomimetic cell membrane models. Microelectrophoretic experiments on two-component liposomes were performed using the electrophoretic light scattering technique (ELS). The effect of both positively (perphenazine, PF) and negatively (barbituric acid, BA) charged drugs on zwitterionic L-α-phosphatidylcholine (PC) membranes were analyzed. Experimental membrane surface charge density (δ) data were determined as a function of pH. Quantitative descriptions of the adsorption equilibria formed due to the binding of solution ions to analyzed two-component membranes are presented. Binding constants of the solution ions with perphenazine and barbituric acid-modified membranes were determined. The results of our research show that both charged drugs change surface charge density values of phosphatidylcholine membranes. It can be concluded that perphenazine and barbituric acid are located near the membrane surface, interacting electrostatically with phosphatidylcholine polar heads.
TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus: Implications for p53 Regulation and Neurite Outgrowth
Authors: Hassan Ahmed , Iftinca Mircea , Young Daniel , Flynn Robyn , Agosti Francina , Abdullah Nasser , Defaye Manon , Scott Mark G. H. , Dufour Antoine , Altier Christophe
Published: February 2021

Transient receptor potential vanilloids (TRPV1) are non-selective cation channels that sense and transduce inflammatory pain signals. We previously reported that activation of TRPV1 induced the translocation of β-arrestin2 (ARRB2) from the cytoplasm to the nucleus, raising questions about the functional role of ARRB2 in the nucleus. Here, we determined the ARRB2 nuclear signalosome by conducting a quantitative proteomic analysis of the nucleus-sequestered L395Q ARRB2 mutant, compared to the cytosolic wild-type ARRB2 (WT ARRB2), in a heterologous expression system. We identified clusters of proteins that localize to the nucleolus and are involved in ribosomal biogenesis. Accordingly, L395Q ARRB2 or WT ARRB2 after capsaicin treatment were found to co-localize and interact with the nucleolar marker nucleophosmin (NPM1), treacle protein (TCOF1) and RNA polymerase I (POL I). We further investigated the role of nuclear ARRB2 signaling in regulating neuroplasticity. Using neuroblastoma (neuro2a) cells and dorsal root ganglia (DRG) neurons, we found that L395Q ARRB2 mutant increased POL I activity, inhibited the tumor suppressorp53 (p53) level and caused a decrease in the outgrowth of neurites. Together, our results suggest that the activation of TRPV1 promotes the ARRB2-mediated regulation of ribosomal biogenesis in the nucleolus. The ARRB2-TCOF1-p53 checkpoint signaling pathway might be involved in regulating neurite outgrowth associated with pathological pain conditions.
Variation, Modification and Engineering of Lipid A in Endotoxin of Gram-Negative Bacteria
Authors: Kawahara Kazuyoshi
Published: February 2021

Lipid A of Gram-negative bacteria is known to represent a central role for the immunological activity of endotoxin. Chemical structure and biosynthetic pathways as well as specific receptors on phagocytic cells had been clarified by the beginning of the 21st century. Although the lipid A of enterobacteria including Escherichia coli share a common structure, other Gram-negative bacteria belonging to various classes of the phylum Proteobacteria and other taxonomical groups show wide variety of lipid A structure with relatively decreased endotoxic activity compared to that of E. coli. The structural diversity is produced from the difference of chain length of 3-hydroxy fatty acids and non-hydroxy fatty acids linked to their hydroxyl groups. In some bacteria, glucosamine in the backbone is substituted by another amino sugar, or phosphate groups bound to the backbone are modified. The variation of structure is also introduced by the enzymes that can modify electrostatic charges or acylation profiles of lipid A during or after its synthesis. Furthermore, lipid A structure can be artificially modified or engineered by the disruption and introduction of biosynthetic genes especially those of acyltransferases. These technologies may produce novel vaccine adjuvants or antagonistic drugs derived from endotoxin in the future.
The RNAi Mechanism Regulates a New Exonuclease Gene Involved in the Virulence of Mucorales
Authors: Pérez-Arques Carlos , Navarro-Mendoza María Isabel , Murcia Laura , Navarro Eusebio , Garre Victoriano , Nicolás Francisco Esteban
Published: February 2021

Mucormycosis is a lethal disease caused by Mucorales, which are emerging as human causes that explain the high mortality for this disease. Consequently, the research community is searching for virulence determinants that could be repurposed as targets to develop new treatments against mucormycosis. Our work explores an RNA interference (RNAi)-based approach to find targets involved in the virulence of Mucorales. A transcriptomewide analysis compared sRNAs and their target mRNAs in two Mucor lusitanicus different pathotypes, virulent and avirulent, generating a list of 75 loci selected by their differential sRNA accumulation in these strains. As a proof of concept and validity, an experimental approach characterized two loci showing opposite behavior, confirming that RNAi activity causes their differential expression in the two pathotypes. We generated deletion mutants for two loci and a knockin-strain overexpressing for one of these loci. Their functional analysis in murine virulence assays identified the gene wex1, a putative DEDDy exonuclease with RNase domains, as an essential factor for virulence. The identification of wex1 showed the potential of our approach to discover virulence factors not only in Mucorales but also in any other fungal model with an active RNAi machinery. More importantly, it adds a new layer to the biological processes controlled by RNAi in M. lusitanicus, confirming that the Dicer-dependent RNAi pathway can silence gene expression to promote virulence.
Whole Blood Thromboelastometry by ROTEM and Thrombin Generation by Genesia According to the Genotype and Clinical Phenotype in Congenital Fibrinogen Disorders
Authors: Szanto Timea , Lassila Riitta , Lemponen Marja , Lehtinen Elina , Neerman-Arbez Marguerite , Casini Alessandro
Published: February 2021

The outcome of congenital fibrinogen defects (CFD) is often unpredictable. Standard coagulation assays fail to predict the clinical phenotype. We aimed to assess the pheno- and genotypic associations of thrombin generation (TG) and ROTEM in CFD. We measured fibrinogen (Fg) activity and antigen, prothrombin fragments F1+2, and TG by ST Genesia® with both Bleed- and ThromboScreen in 22 patients. ROTEM was available for 11 patients. All patients were genotyped for fibrinogen mutations. Ten patients were diagnosed with hypofibrinogenemia, nine with dysfibrinogenemia, and three with hypodysfibrinogenemia. Among the 17 mutations, eight were affecting the Fg γ chain, four the Fg Bβ chain, and five the Fg Aα chain. No statistical difference according to the clinical phenotypes was observed among FGG and FGA mutations. Median F1+2 and TG levels were normal among the different groups. Fg levels correlated negatively with F1+2 and peak height, and positively with lag time and time to peak. The pheno- and genotypes of the patients did not associate with TG. FIBTEM by ROTEM detected hypofibrinogenemia. Our study suggests an inverse link between low fibrinogen activity levels and enhanced TG, which could modify the structure–function relationship of fibrin to support hemostasis.
Spinal Excitatory Dynorphinergic Interneurons Contribute to Burn Injury-Induced Nociception Mediated by Phosphorylated Histone 3 at Serine 10 in Rodents
Authors: Varga Angelika , Mészár Zoltán , Sivadó Miklós , Bácskai Tímea , Végh Bence , Kókai Éva , Nagy István , Szücs Péter
Published: February 2021

The phosphorylation of serine 10 in histone 3 (p-S10H3) has recently been demonstrated to participate in spinal nociceptive processing. However, superficial dorsal horn (SDH) neurons involved in p-S10H3-mediated nociception have not been fully characterized. In the present work, we combined immunohistochemistry, in situ hybridization with the retrograde labeling of projection neurons to reveal the subset of dorsal horn neurons presenting an elevated level of p-S10H3 in response to noxious heat (60 °C), causing burn injury. Projection neurons only represented a small percentage (5%) of p-S10H3-positive cells, while the greater part of them belonged to excitatory SDH interneurons. The combined immunolabeling of p-S10H3 with markers of already established interneuronal classes of the SDH revealed that the largest subset of neurons with burn injury-induced p-S10H3 expression was dynorphin immunopositive in mice. Furthermore, the majority of p-S10H3-expressing dynorphinergic neurons proved to be excitatory, as they lacked Pax-2 and showed Lmx1b-immunopositivity. Thus, we showed that neurochemically heterogeneous SDH neurons exhibit the upregulation of p-S10H3 shortly after noxious heat-induced burn injury and consequential tissue damage, and that a dedicated subset of excitatory dynorphinergic neurons is likely a key player in the development of central sensitization via the p-S10H3 mediated pathway.
Targeting the Renin–Angiotensin–Aldosterone System to Prevent Hypertension and Kidney Disease of Developmental Origins
Authors: Hsu Chien-Ning , Tain You-Lin
Published: February 2021

The renin-angiotensin-aldosterone system (RAAS) is implicated in hypertension and kidney disease. The developing kidney can be programmed by various early-life insults by so-called renal programming, resulting in hypertension and kidney disease in adulthood. This theory is known as developmental origins of health and disease (DOHaD). Conversely, early RAAS-based interventions could reverse program processes to prevent a disease from occurring by so-called reprogramming. In the current review, we mainly summarize (1) the current knowledge on the RAAS implicated in renal programming; (2) current evidence supporting the connections between the aberrant RAAS and other mechanisms behind renal programming, such as oxidative stress, nitric oxide deficiency, epigenetic regulation, and gut microbiota dysbiosis; and (3) an overview of how RAAS-based reprogramming interventions may prevent hypertension and kidney disease of developmental origins. To accelerate the transition of RAAS-based interventions for prevention of hypertension and kidney disease, an extended comprehension of the RAAS implicated in renal programming is needed, as well as a greater focus on further clinical translation.