bluuebunny/crossref_metadata_2025 huggingface dataset:
Virtual screening and activity evaluation of multitargeting inhibitors for idiopathic pulmonary fibrosis
Authors: Wang Rui , Xu Jian , Yan Rong , Liu Huanbin , Zhao Jingxin , Xie Yuan , Deng Wenbin , Liao Weiping , Nie Yichu
Published: September 2022

Transforming growth factor β receptor (TGF-β1R) and receptor tyrosine kinases (RTKs), such as VEGFRs, PDGFRs and FGFRs are considered important therapeutic targets in blocking myofibroblast migration and activation of idiopathic pulmonary fibrosis (IPF). To screen and design innovative prodrug to simultaneously target these four classes of receptors, we proposed an approach based on network pharmacology combining virtual screening and machine learning activity prediction, followed by efficient in vitro and in vivo models to evaluate drug activity. We first constructed Collagen1A2-A549 cells with type I collagen as the main biomarker and evaluated the activity of compounds to inhibit collagen expression at the cellular level. The data from the first round of Collagen1A2-A549 cell screening were substituted into the machine learning model, and the model was optimized accordingly. As a result, the false positive rate of the model was reduced from 85.0% to 66.7%, and two prospective compounds, Z103080500 and Z104578368, were finally selected. Collagen levels were reduced effectively by both Z103080500 (67.88% reduction) and Z104578368 (69.54% reduction). Moreover, these two compounds showed low cellular cytotoxicity. Subsequently, the effect of Z103080500 and Z104578368 was evaluated in a bleomycin-induced C57BL/6 mouse IPF model. These results showed that 50 mg/kg Z103080500 and Z104578368 could effectively reduce the number of inflammatory cells and the expression level of α-SMA. Meanwhile, Z103080500 and Z104578368 reduced the expression of major markers and inflammatory factors of IPF, such as collagen, IFN-γ, IL-17 and HYP, indicating that these screened Z103080500 and Z104578368 effectively delayed lung tissue inflammation and had a potential therapeutic effect on IPF. Our findings demonstrate that a screening and evaluation model for prodrug against IPF has been successfully established. It is of great significance to further modify these compounds to enhance their potency and activity.
Basins of attraction of chimera states on networks
Authors: Li Qiang , Larosz Kelly C. , Han Dingding , Ji Peng , Kurths Jürgen
Published: September 2022

Networks of identical coupled oscillators display a remarkable spatiotemporal pattern, the chimera state, where coherent oscillations coexist with incoherent ones. In this paper we show quantitatively in terms of basin stability that stable and breathing chimera states in the original two coupled networks typically have very small basins of attraction. In fact, the original system is dominated by periodic and quasi-periodic chimera states, in strong contrast to the model after reduction, which can not be uncovered by the Ott-Antonsen ansatz. Moreover, we demonstrate that the curve of the basin stability behaves bimodally after the system being subjected to even large perturbations. Finally, we investigate the emergence of chimera states in brain network, through inducing perturbations by stimulating brain regions. The emerged chimera states are quantified by Kuramoto order parameter and chimera index, and results show a weak and negative correlation between these two metrics.
Caloric restriction prevents obesity- and intermittent hypoxia-induced cardiac remodeling in leptin-deficient ob/ob mice
Authors: Jones Aaron A. , Framnes-DeBoer Sarah N. , Shipp Arianne , Arble Deanna M.
Published: September 2022

Background: Intermittent hypoxia (IH), a key characteristic of obstructive sleep apnea, is independently associated with cardiometabolic impairment. While endogenous leptin levels may provide cardioprotective effects against hypoxia, leptin resistance is common among obese individuals presenting with obstructive sleep apnea.

Methods: Here, we assessed left ventricle (LV) function using M-mode echocardiography in lean wild-type, calorically-restricted ob/ob, and obese ob/ob mice before and after 6 days of IH to determine how obesity and intermittent hypoxia interact to affect cardiac function independent of leptin signaling.

Results: Calorically-restricting ob/ob mice for 4 weeks prior to IH exposure prevented weight gain (−2.1 ± 1.4 g) compared to free-fed ob/ob mice (8.7 ± 1.1 g). Free-fed ob/ob mice exhibited increased LV mass (0.713 ± 0.008 g) relative to wild-type mice (0.685 ± 0.004 g) and increased posterior wall thickness (0.089 ± 0.006 cm) relative to calorically-restricted ob/ob mice (0.072 ± 0.004 cm). Following 6 days of IH, free-fed ob/ob mice exhibited increases in cardiac output (44.81 ± 2.97 pre-IH vs. 57.14 ± 3.09 ml/min post-IH), LV diameter (0.400 ± 0.007 pre-IH vs. 0.428 ± 0.009 cm post-IH) and end diastolic volume (0.160 ± 0.007 pre-IH vs. 0.195 ± 0.012 ml post-IH) that were not detected in wild-type or calorically-restricted ob/ob mice.

Conclusion: Caloric restriction can prevent obesity-induced LV hypertrophy and protect against acute IH-induced cardiac remodeling independent of leptin signaling. These findings may have clinical implications for obstructive sleep apnea.
Assessing the effects of artificial gravity in an analog of long-duration spaceflight: The protocol and implementation of the AGBRESA bed rest study
Authors: Clément Gilles , Rittweger Jörn , Nitsche Andrea , Doering Wolfgang , Frings-Meuthen Petra , Hand Olga , Frett Timo , Noppe Alexandra , Paulke Freia , Lecheler Leopold , Jordan Jens , Stern Claudia , Mulder Edwin
Published: September 2022

A comprehensive strategy is required to mitigate risks to astronauts’ health, well-being, and performance. This strategy includes developing countermeasures to prevent or reduce adverse responses to the stressors astronauts encounter during spaceflight, such as weightlessness. Because artificial gravity (AG) by centrifugation simultaneously affects all physiological systems, AG could mitigate the effects of weightlessness in multiple systems. In 2019, NASA and the German Aerospace Center conducted a 60-days Artificial Gravity Bed Rest Study with the European Space Agency (AGBRESA). The objectives of this study were to 1) determine if 30 min of AG daily is protective during head down bed rest, and 2) compare the protective effects of a single daily bout (30 min) of AG versus multiple daily bouts (6 × 5 min) of AG (1 Gz at the center of mass) on physiological functions that are affected by weightlessness and by head-down tilt bed rest. The AGBRESA study involved a comprehensive suite of standard and innovative technologies to characterize changes in a broad spectrum of physiological systems. The current article is intended to provide a detailed overview of the methods used during AGBRESA.
Visuomotor performance at high altitude in COPD patients. Randomized placebo-controlled trial of acetazolamide
Authors: Scheiwiller P. M. , Furian M. , Buergin A. , Mayer L. C. , Schneider S. R. , Mademilov M. , Lichtblau M. , Muralt L. , Sheraliev U. , Sooronbaev T. M. , Ulrich S. , Bloch K. E.
Published: September 2022

Introduction: We evaluated whether exposure to high altitude impairs visuomotor learning in lowlanders with chronic obstructive pulmonary disease (COPD) and whether this can be prevented by acetazolamide treatment.

Methods: 45 patients with COPD, living <800 m, FEV1 ≥40 to <80%predicted, were randomized to acetazolamide (375 mg/d) or placebo, administered 24h before and during a 2-day stay in a clinic at 3100 m. Visuomotor performance was evaluated with a validated, computer-assisted test (Motor-Task-Manager) at 760 m above sea level (baseline, before starting the study drug), within 4h after arrival at 3100 m and in the morning after one night at 3100 m. Main outcome was the directional error (DE) of cursor movements controlled by the participant via mouse on a computer screen during a target tracking task. Effects of high altitude and acetazolamide on DE during an adaptation phase, immediate recall and post-sleep recall were evaluated by regression analyses. www.ClinicalTrials.gov NCT03165890.

Results: In 22 patients receiving placebo, DE at 3100 m increased during adaptation by mean 2.5°, 95%CI 2.2° to 2.7° (p < 0.001), during immediate recall by 5.3°, 4.6° to 6.1° (p < 0.001), and post-sleep recall by 5.8°, 5.0 to 6.7° (p < 0.001), vs. corresponding values at 760 m. In 23 participants receiving acetazolamide, corresponding DE were reduced by −0.3° (−0.6° to 0.1°, p = 0.120), −2.7° (−3.7° to −1.6°, p < 0.001) and −3.1° (−4.3° to −2.0°, p < 0.001), compared to placebo at 3100 m.

Conclusion: Lowlanders with COPD travelling to 3100 m experienced altitude-induced impairments in immediate and post-sleep recall of a visuomotor task. Preventive acetazolamide treatment mitigated these undesirable effects.
Evaluation and identification of advanced lentil interspecific derivatives resulted in the development of early maturing, high yielding, and disease-resistant cultivars under Indian agro-ecological conditions
Authors: Singh Mohar , Kumar Sanjeev , Mehra Reena , Sood Salej , Malhotra Nikhil , Sinha Reena , Jamwal Sonika , Gupta Vikas
Published: September 2022

The genetic base revealed by pedigree records of the majority of released cultivars appears to be narrow in major pulse crops, including lentils, because of the frequent use of the same parents and their derivatives in crop improvement programs. Therefore, corrective measures are needed to widen the genetic base by involving the genetic resources of a distinct gene pool. In this direction, rigorous efforts were made to introgress wild Lens taxa, L. culinaris ssp. orientalis, and L. ervoides into the backgrounds of cultivated varieties. Subsequently, genetic materials were advanced through the single seed descent method of breeding along with a rapid generation advancement (normal and off-season) approach. Two F10:11 interspecific derivatives of lentils were evaluated in augmented block design at two locations, viz. International Centre for Agricultural Research in Dry Areas (ICARDA) and Sher-e-Kashmir University of Agricultural Sciences and Technology (SKUAST), India. The analysis of variance showed remarkable variability for all target characters at both locations. The heritability estimates were high, and correlation analysis exhibited a significant association between the majority of traits assessed at ICARDA and SKUAST, India. Further, SKUAST identified the most promising lines as “Jammu Lentil 144” and “Jammu Lentil 71.” These derivatives were further validated separately for their agronomic potential and resistance against major biotic stresses. The results revealed that Jammu Lentil 144 and Jammu Lentil 71 produced 16.65 and 9.40% more seed yield than local and national checks, including earliness, by 25 and 15 days, respectively. These promising interspecific derivatives were also found to be resistant to fusarium wilt, root rot, pod borer, and aphid infestations. The standard agronomy of these cultivars has also been assessed consecutively for 2 years at SKUAST. Overall, the pre-breeding efforts have resulted in the development of early maturing, high-yielding, and disease-resistant lentil cultivars for the Jammu region of India.
Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant
Authors: Chacon Daisy Sotero , Santos Marlon Dias Mariano , Bonilauri Bernardo , Vilasboa Johnatan , da Costa Cibele Tesser , da Silva Ivanice Bezerra , Torres Taffarel de Melo , de Araújo Thiago Ferreira , Roque Alan de Araújo , Pilon Alan Cesar , Selegatto Denise Medeiros , Freire Rafael Teixeira , Reginaldo Fernanda Priscila Santos , Voigt Eduardo Luiz , Zuanazzi José Angelo Silveira , Scortecci Kátia Castanho , Cavalheiro Alberto José , Lopes Norberto Peporine , Ferreira Leandro De Santis , Santos Leandro Vieira dos , Fontes Wagner , Sousa Marcelo Valle de , Carvalho Paulo Costa , Fett-Neto Arthur Germano , Giordani Raquel Brandt
Published: September 2022

Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism in plants inhabiting the harsh conditions of the Caatinga, the present study focused on this important class of bioactive phenolics. Leaves and seeds of plants growing in their natural habitat had their metabolic and proteomic profiles analyzed and integrated with transcriptome data. As a result, 96 metabolites (including 43 flavonoids) were annotated. Transcripts of the flavonoid pathway totaled 27, of which EvCHI, EvCHR, EvCHS, EvCYP75A and EvCYP75B1 were identified as putative main targets for modulating the accumulation of these metabolites. The highest correspondence of mRNA vs. protein was observed in the differentially expressed transcripts. In addition, 394 candidate transcripts encoding for transcription factors distributed among the bHLH, ERF, and MYB families were annotated. Based on interaction network analyses, several putative genes of the flavonoid pathway and transcription factors were related, particularly TFs of the MYB family. Expression patterns of transcripts involved in flavonoid biosynthesis and those involved in responses to biotic and abiotic stresses were discussed in detail. Overall, these findings provide a base for the understanding of molecular and metabolic responses in this medicinally important species. Moreover, the identification of key regulatory targets for future studies aiming at bioactive metabolite production will be facilitated.
Plastome sequences fail to resolve shallow level relationships within the rapidly radiated genus Isodon (Lamiaceae)
Authors: Chen Ya-Ping , Zhao Fei , Paton Alan J. , Sunojkumar Purayidathkandy , Gao Lian-Ming , Xiang Chun-Lei
Published: September 2022

As one of the largest genera of Lamiaceae and of great medicinal importance, Isodon is also phylogenetically and taxonomically recalcitrant largely ascribed to its recent rapid radiation in the Hengduan Mountains. Previous molecular phylogenetic studies using limited loci have only successfully resolved the backbone topology of the genus, but the interspecific relationships suffered from low resolution, especially within the largest clade (Clade IV) which comprises over 80% species. In this study, we attempted to further elucidate the phylogenetic relationships within Isodon especially Clade IV using plastome sequences with a broad taxon sampling of ca. 80% species of the genus. To reduce systematic errors, twelve different plastome data sets (coding and non-coding regions with ambiguously aligned regions and saturated loci removed or not) were employed to reconstruct phylogeny using maximum likelihood and Bayesian inference. Our results revealed largely congruent topologies of the 12 data sets and recovered major lineages of Isodon consistent with previous studies, but several incongruences are also found among these data sets and among single plastid loci. Most of the shallow nodes within Clade IV were resolved with high support but extremely short branch lengths in plastid trees, and showed tremendous conflicts with the nrDNA tree, morphology and geographic distribution. These incongruences may largely result from stochasticity (due to insufficient phylogenetic signal) and hybridization and plastid capture. Therefore, the uniparental-inherited plastome sequences are insufficient to disentangle relationships within a genus which has undergone recent rapid diversification. Our findings highlight a need for additional data from nuclear genome to resolve the relationships within Clade IV and more focused studies to assess the influences of multiple processes in the evolutionary history of Isodon. Nevertheless, the morphology of the shape and surface sculpture/indumentum of nutlets is of systematic importance that they can distinguish the four major clades of Isodon.
Determining the critical factors of eWOM about corporate social responsibility on social networking sites: End users’ perspective
Authors: Hu Yuchen , Tang Qingbo , Wang Xuan , Ali Shahid
Published: September 2022

It is now possible to propagate CSR information through social media platforms. Electronic word of mouth (eWOM) directly impacts image and upcoming portfolios of the organization. Customers, employees, and other stakeholders generate revenue for the company. Our goal was to understand why people were sharing and commenting in response to terrible reports about corporate social responsibility (CSR) on WeChat. A company’s desire to comment on and share CSR news and its perception of its own social and environmental responsibility were all presumed explanatory variables in our investigation. 315 WeChat users were asked to grade a fictitious news report of the environment. The results were shocking. According to our findings, an individual’s attitude toward actions and the effectiveness of information directly correlates to their social and environmental awareness level. EWOM may be discouraged by a company’s brand name, which has the potential to harm its reputation with its customers.
The biological dimensions of transcendent states: A randomized controlled trial
Authors: Church Dawson , Yang Amy , Fannin Jeffrey , Blickheuser Katharina
Published: September 2022

This study evaluated the biological dimension of meditation and self-transcendent states. A convenience sample of 513 participants was drawn from attendees at a 4-day guided meditation workshop. Half were randomly assigned to an active placebo control intervention. All were assessed on a variety of measures, both psychological [anxiety, pain, posttraumatic stress disorder (PTSD), positive emotions, and transcendent states], and physiological (physical functioning). Additional biological assessments including salivary immunoglobulin-A (SIgA), cortisol, and Quantitative Electroencephalography (qEEG) were obtained from subset of the Experimental group (N = 117). No significant difference in psychological symptoms or positive emotions was observed between Experimental and placebo groups at baseline. At post-test, significant improvements were noted in the Experimental group, including a 49.5% median increase in SIgA (p = 0.01), though cortisol remained unchanged. qEEG z-score analysis identified sustained stress reduction, including delta frequency band amplitude increases, high beta decreases, and faster acquisition of sustained alpha states (all p < 0.001). Psychological symptoms also improved on all measures. At 6-month follow-up (N = 140), PTSD and somatic symptoms significantly improved from baseline, and post-test versus 6-month follow-up results indicated significant increases in happiness and spiritual and physical oneness, along with decreases in depressive symptoms. These findings suggest that autonomic self-regulation and transcendent states may be measured in both biological and psychological dimensions and are associated with pervasive health benefits.
The effects of perception of video image and online word of mouth on tourists’ travel intentions: Based on the behaviors of short video platform users
Authors: Zhou Yang , Liu Ligang , Sun Xiao
Published: September 2022

This research discusses the impact of the perception of video images and online word of mouth on tourists’ travel intentions. A survey of 390 users who watched travel videos on short-video platforms was conducted using structural equation modeling. The results are as follows. First, the perception of video images can significantly affect tourists’ intention to visit the destinations. Second, as a mediating variable, online word of mouth can enhance the positive effects of the perception of video images on tourists’ travel intentions. Third, gender had a positive moderating effect, which was particularly obvious in the relationship between the perception of video images and online word of mouth. This research provides a theoretical basis for the utility of tourism-related short videos, which can help stimulate tourists’ intention to visit promoted destinations.
Outcomes associated with antidepressant treatment according to the number of prescriptions and treatment changes: 5-year follow-up of a nation-wide cohort study
Authors: Ouazana-Vedrines Charles , Lesuffleur Thomas , Cuerq Anne , Fagot-Campagna Anne , Rachas Antoine , Gastaldi-Ménager Chrystelle , Hoertel Nicolas , Limosin Frédéric , Lemogne Cédric , Tuppin Philippe
Published: September 2022

Background

Naturalistic studies regarding clinical outcomes associated with antidepressant treatment duration have yielded conflicting results, possibly because they did not consider the occurrence of treatment changes. This nation-wide population-based study examined the association between the number of filled prescriptions and treatment changes and long-term psychiatric outcomes after antidepressant treatment initiation.

Methods

Based on the French national health insurance database, 842,175 adults who initiated an antidepressant treatment in 2011 were included. Cox proportional-hazard multi-adjusted regression models examined the association between the number of filled prescriptions and the occurrence of treatment changes 12 months after initiation and four outcomes during a 5-year follow-up: psychiatric hospitalizations, suicide attempts, sick leaves for a psychiatric diagnosis, new episodes of antidepressant treatment.

Results

During a mean follow-up of 4.5 years, the incidence rates of the four above-mentioned outcomes were 13.49, 2.47, 4.57, and 92.76 per 1,000 person-years, respectively. The number of filled prescriptions was associated with each outcome (adjusted HRs [95% CI] for one additional prescription ranging from 1.01 [1.00–1.02] to 1.10 [1.09–1.11]), as was the occurrence of at least one treatment change vs. none (adjusted HRs [95% CI] ranging from 1.18 [1.16–1.21] to 1.57 [1.79–1.65]). Furthermore, the adjusted HRs [95% CI] of the number of filled prescriptions were greater in patients with (vs. without) a treatment change for psychiatric hospitalizations (1.12 [1.11–1.14] vs. 1.09 [1.08–1.10], p for interaction = 0.002) and suicide attempts (1.12 [1.09–1.15] vs. 1.06 [1.04–1.08], p for interaction = 0.006).

Limitations

Lack of clinical data about the disorders warranting the prescriptions or their severity.

Conclusion

Considering treatment changes is critical when using administrative claims database to examine the long-term psychiatric outcomes of antidepressant treatments in real-life settings.
Longitudinal trends in the prevalence and treatment of depression among adults with cardiovascular disease: An analysis of national health and nutrition examination survey 2009–2020
Authors: Feng Zhen , Tong Wai Kei , Tang Zhijia
Published: September 2022

Objectives

To assess the contemporary prevalence and decadal trends of depression and antidepressant use among adults with cardiovascular disease (CVD) in the United States, as well as their risk factors from 2009 to 2020.

Materials and methods

We used the National Health and Nutrition Examination Survey data to calculate the weighted prevalence of depression and antidepressant use. Adults aged 20 years or older with CVD were included. Depression and CVD were assessed by the Patient Health Questionnaire (PHQ-9) and self-report, respectively.

Results

A total of 3,073 eligible participants with CVD aged >20 years were included. The overall prevalence of depression defined by PHQ-9 score ≥10 was 15.7% (95% CI 13.8–17.5), with a steady trend during 2009–March 2020 (p = 0.777). Female gender (aOR 1.78, 95% CI 1.20–2.64) and sleep disorder (aOR 2.62, 95% CI 1.78–3.86) were independent risk factors for depression in CVD patients, while high education level, high income, longer sleep duration, and non-current smokers were considered protective factors. The weighted prevalence of antidepressant use among depressed patients with CVD was 38.6%, which also remained unchanged during the survey period (p = 0.699). Participants with normal sleep pattern and duration were significantly less likely to take antidepressants (p = 0.003).

Conclusion

The longitudinal trends in the prevalence of depression among CVD patients in the United States have been stable over the past decade, despite being significantly higher in women, and those with sleep disorders. Overall, antidepressant use was fairly low. Aggressive screening and tailored treatment are recommended for specific vulnerable subpopulations to improve their clinical outcomes.
Unraveling the effect of ambivalence over emotional expression on subjective wellbeing in Chinese college students in romantic relationships: A multiple mediation model
Authors: Wang Yue , Zheng Zexin , Wang Xiaoyu , Li Ying
Published: September 2022

The sudden and unpredictable outbreak of the COVID-19 pandemic has severely threatened young adults’ physical and mental health and damaged the quality of relationships. As a critical stage of development, their well-being is more vulnerable to adverse environments which may lead to profound negative long-life mental health status. The current study aimed to investigate the mediation effects of fear of intimacy and attachment avoidance in the association between ambivalence over emotional expression and subjective wellbeing. A sample of 555 Chinese college students who are currently involved in romantic relationships (Mean age = 19.69, SD age = 1.36, 52% females) completed anonymous questionnaires regarding ambivalence over emotional expression, fear of intimacy, attachment avoidance, and subjective wellbeing. The findings revealed that ambivalence over emotional expression can predict negative subjective wellbeing of college students in romantic relationships significantly, and fear of intimacy and attachment avoidance played a sequentially mediating role in the association between ambivalence over emotional expression and subjective wellbeing. Surprisingly, when considering attachment avoidance independently, we found it had an inhibitory action on the link between ambivalence over emotional expression and subjective wellbeing. The present study contributes to a better understanding of how ambivalence over emotional expression can decrease subjective wellbeing, and also has implications for the intervention of students’ subjective wellbeing and closeness during the period of COVID-19.
Improving timeliness and completion of infant vaccination among infants in Nigerian urban slums through older women's participation
Authors: Balogun Folusho Mubowale , Bamgboye Eniola Adetola , Akindolire Abimbola Ellen
Published: September 2022

Nigerian urban slums have a high population of infants with suboptimal vaccination despite previous interventions. Older women traditionally play supervisory roles in infant care in Nigeria but their influence is untapped in infant vaccination. This study sought to determine if training of older women (≥35 years) in urban slum communities in Ibadan, South west Nigeria, and involving them in infant vaccination will improve infant vaccination timeliness and completion. This was a randomized experimental community study and pregnant women in their third trimester, residing in seven urban slum communities were randomized using their antenatal clinics (ANCs) into intervention (six ANCs) and control groups (six ANCs). The older women who will supervise the care of the infants of pregnant women in the intervention group had seven sessions of training on the importance of infant vaccination timeliness and completion. The vaccinations of the infants from both groups were compared from birth till 9 months. Data were analyzed using descriptive statistics and Chi square test at α = 0.05. There were 96 older women, 198 pregnant women (105 in intervention group and 93 controls) and 202 infants (109 in intervention group and 93 controls). Infants in the intervention group (67.9%) significantly had both timely and complete vaccinations compared with those in the control group (36.6%). Vaccines given at birth were the least timely in both groups. More infants whose older women caregiver were married had timely and complete vaccinations. Also, a higher proportion of male infants, low birth weight babies and infants with older women caregiver with at most two children had timely and completed vaccinations but these were not statistically significant. Training of older women caregivers improved infant vaccination timeliness and completion in these urban slum communities. This model may improve infant vaccination in other similar urban slum settings.
Concurrent validity of the combined HRV/ACC sensor and physical activity diary when monitoring physical activity in university students during free-living days
Authors: Liu Haochong , Li Qian , Li Yiting , Wang Yubo , Huang Yaling , Bao Dapeng , Liu Haoyang , Cui Yixiong
Published: September 2022

The purpose of this research was to determine if the scientific research device combined heart rate variability combined with an acceleration sensor (Firstbeat Bodyguard 2, BG2) was valid and reliable for time spent in different intensity zones in free-living. A total of 55 healthy participants performed 48-h physical activity (PA) monitoring with BG2, ActiGraph GT3X+ (GT3X+), and completed Bouchard Physical Activity Diary (Bouchard) every night. In the available studies, GT3X+ is considered the gold standard scientific research device for PA monitor. We compared BG2 and Bouchard with GT3X+ by difference, correlation, and agreement of PA and energy expenditure (EE) in free-living. The results showed that BG2 estimated PA more accurately than Bouchard, with a modest correlation (r > 0.49), strong agreement (τ > 0.29), and they had the lowest limits of agreement when estimating moderate to vigorous physical activity (MVPA). The EE estimated by Bouchard was the highest among the three methods, and the correlation and agreement between the three methods were high. Our findings showed that the BG2 is valid and reliable for estimating time spent in different intensity zones in free-living, especially in MVPA.
Impacts of risk assessment data, assumptions, and methods: Considering the evidence for diacetyl and 2,3-pentanedione
Authors: Johns Douglas O. , Whittaker Christine , Cox-Ganser Jean M.
Published: September 2022

The articles published as part of the Frontiers in Public Health research topic, “Investigating exposures and respiratory health in coffee workers” present research findings that better characterize exposures to diacetyl and 2,3-pentanedione and inform our understanding of the health risks posed by these exposures. Although various research groups and organizations have conducted risk assessments to derive occupational exposure limits (OELs) for diacetyl, differences in the data used and assumptions made in these efforts have resulted in a wide range of recommended OELs designed to protect human health. The primary drivers of these differences include the decision to use data from human or animal studies in conducting a quantitative risk assessment, and the application of uncertainty factors (UF) to derive an OEL. This Perspectives paper will discuss the practical implications of these decisions, and present additional commentary on the potential role that the recent investigation of human exposures to relatively low concentrations of α-diketones, specifically diacetyl and 2,3-pentanedione, may play in supporting qualitative or quantitative human health risk assessments.
Integrated design-sense-plan architecture for autonomous geometric-semantic mapping with UAVs
Authors: Pimentel de Figueiredo Rui , Le Fevre Sejersen Jonas , Grimm Hansen Jakob , Brandão Martim
Published: September 2022

This article presents a complete solution for autonomous mapping and inspection tasks, namely a lightweight multi-camera drone design coupled with computationally efficient planning algorithms and environment representations for enhanced autonomous navigation in exploration and mapping tasks. The proposed system utilizes state-of-the-art Next-Best-View (NBV) planning techniques, with geometric and semantic segmentation information computed with Deep Convolutional Neural Networks (DCNNs) to improve the environment map representation. The main contributions of this article are the following. First, we propose a novel efficient sensor observation model and a utility function that encodes the expected information gains from observations taken from specific viewpoints. Second, we propose a reward function that incorporates both geometric and semantic probabilistic information provided by a DCNN for semantic segmentation that operates in close to real-time. The incorporation of semantics in the environment representation enables biasing exploration towards specific object categories while disregarding task-irrelevant ones during path planning. Experiments in both a virtual and a real scenario demonstrate the benefits on reconstruction accuracy of using semantics for biasing exploration towards task-relevant objects, when compared with purely geometric state-of-the-art methods. Finally, we present a unified approach for the selection of the number of cameras on a UAV, to optimize the balance between power consumption, flight-time duration, and exploration and mapping performance trade-offs. Unlike previous design optimization approaches, our method is couples with the sense and plan algorithms. The proposed system and general formulations can be be applied in the mapping, exploration, and inspection of any type of environment, as long as environment dependent semantic training data are available, with demonstrated successful applicability in the inspection of dry dock shipyard environments.
Clinical laboratory diagnostics in dentistry: Application of microbiological methods
Authors: Claesson Rolf , Johansson Anders , Belibasakis Georgios N.
Published: September 2022

Diagnosis and treatment in dentistry are based on clinical examination of the patients. Given that the major oral diseases are of microbial biofilm etiology, it can be expected that performing microbiological analysis on samples collected from the patient could deliver supportive evidence to facilitate the decision-making process by the clinician. Applicable microbiological methods range from microscopy, to culture, to molecular techniques, which can be performed easily within dedicated laboratories proximal to the clinics, such as ones in academic dental institutions. Periodontal and endodontic infections, along with odontogenic abscesses, have been identified as conditions in which applied clinical microbiology may be beneficial for the patient. Administration of antimicrobial agents, backed by microbiological analysis, can yield more predictable treatment outcomes in refractory or early-occurring forms of periodontitis. Confirming a sterile root canal using a culture-negative sample during endodontic treatment may ensure the longevity of its outcome and prevent secondary infections. Susceptibility testing of samples obtained from odontogenic abscesses may facilitate the selection of the appropriate antimicrobial treatment to prevent further spread of the infection.
How Do Emotions Affect Sport Performance?
Authors: Tamminen Katherine A. , Dunn Rachel
Published: September 2022

Playing sports can be filled with emotions. Athletes might feel nervous about try-outs or before a big competition, upset about losing or performing poorly, or excited and happy after a big win or a major accomplishment. To perform well in sport, athletes can learn to manage their emotions and cope with stress. However, some emotion regulation and coping strategies might be more useful than others. So, how can athletes learn to deal with their emotions and cope with stress in sport? In this article, we review what emotions are and how they influence athletes’ thoughts and behaviors in sport, as well as their impact on sport performance. We also review types of coping strategies that athletes can use to deal with stress and emotions in sport, and we explore ways that athletes can learn to develop better coping skills for sport performance.
Development and trainability of agility in youth: A systematic scoping review
Authors: Thieschäfer Lutz , Büsch Dirk
Published: September 2022

Background

Agility is acknowledged as a crucial factor of performance in various open skill sports in both adult and youth athletes. However, despite its significance for sports performance the development and the trainability of agility are under-researched within the pediatric literature. A systematic scoping review was considered most appropriate to provide researchers and practitioners with an overview of the current body of literature approaching agility in youth.

Objectives

The objectives of this scoping review were to map the extent, range, and nature of existing evidence regarding trainability and “natural” development of agility and to summarize corresponding study results.

Methods

The scoping review protocol was pre-registered at Open Science Framework. Systematic searches were conducted using the databases PubMed, Scopus, ProQuest, Web of Science, SURF, and SPONET to identify sources covering agility in youth. Among other inclusion criteria, only references applying unplanned agility concepts were included.

Results

Ultimately, 41 reports were included comprising 23 observational studies, 14 experimental studies, and 4 references of secondary research. A total of 3,087 subjects were assessed in the included studies. Subject groups were predominantly male, above 10 years of age, and soccer athletes. Outcomes of observational studies indicate an effect of age and maturation on agility performance resulting in a non-linear “natural” development of agility. Furthermore, relationships between contributing perceptual-cognitive factors and agility performance tend to increase with progressing age, whereas relationships between physical factors and agility performance diminish. Evidence of training studies suggests that agility is trainable in youth, albeit with various underlying mechanisms.

Conclusions

This systematic scoping review is the first mapping of the body of literature about agility in youth. It outlines the current evidence base, reveals research gaps, and points out future directions to support researchers and practitioners in this field. Although, increasing research activity in this field is discernible, agility research in youth is still in its infancy. Considering the significance of agility for sports performance, future research is postulated to design evidence-based strategies for long-term agility development in young athletes.
More time for judo matches? Analysis of type of techniques, time, scores, and penalties in the Tokyo 2020 Olympic Games
Authors: Kons Rafael Lima , Agostinho Marcus F. , Lopes-Silva João Paulo , Conceição dos Santos Danilo França , Detanico Daniele , Franchini Emerson
Published: September 2022

This study aimed to analyze the distribution of judo matches in the Tokyo 2020 Olympic Games, considering matches in the regular duration (≤4 min) and during the extra time (the golden score) according to sex, scores, penalties, phases of competition, weight categories, and judo techniques. The data were extracted from the Official Results Books of the Olympic Games in Tokyo 2020. The combats were divided in matches that were finished in the regular duration and that were finished by the golden score, with a total of 450 matches. The Chi-square test was used to identify the association between all variables and different groups of matches duration, with the level at p < 0.05. The main results demonstrated significant association, considering sex and frequency of matches in the regular time (χ2 = 6.59; p = 0.010) for female athletes. The majority of matches ended in the 3–4 min (χ2 = 204.16; p < 0.001), scores were mostly awarded in the regular time and penalties in the golden score (χ2 = 48.72; p < 0.001), and few matches ended by the golden score for heavyweight athletes (χ2 = 15.16; p = 0.019). Therefore, a high number of matches ended in the golden score in the Olympic Games Tokyo 2020, with a high number of penalties in this competition.
Expression characteristics of piRNAs in ovine luteal phase and follicular phase ovaries
Authors: Li Chunyan , Zhang Rensen , Zhang Zijun , Ren Chunhuan , Wang Xiangyu , He Xiaoyun , Mwacharo Joram Mwashigadi , Zhang Xiaosheng , Zhang Jinlong , Di Ran , Chu Mingxing
Published: September 2022

PIWI-interacting RNAs (piRNAs), as a novel class of small non-coding RNAs that have been shown to be indispensable in germline integrity and stem cell development. However, the expressed characteristics and regulatory roles of piRNAs during different reproductive phases of animals remain unknown. In this study, we investigated the piRNAs expression profiles in ovaries of sheep during the luteal phase (LP) and follicular phase (FP) using the Solexa sequencing technique. A total of 85,219 and 1,27,156 piRNAs tags were identified in ovine ovaries across the two phases. Most expressed piRNAs start with uracil. piRNAs with a length of 24 nt or 27–29 nts accounted for the largest proportion. The obvious ping-pong signature appeared in the FP ovary. The piRNA clusters in the sheep ovary were unevenly distributed on the chromosomes, with high density on Chr 3 and 1. For genome distribution, piRNAs in sheep ovary were mainly derived from intron, CDS, and repeat sequence regions. Compared to the LP ovary, a greater number of expressed piRNA clusters were detected in the FP ovary. Simultaneously, we identified 271 differentially expressed (DE) piRNAs between LP and FP ovaries, with 96 piRNAs upregulated and 175 piRNAs downregulated, respectively. Functional enrichment analysis (GO and KEGG) indicated that their target genes were enriched in reproduction-related pathways including oocyte meiosis, PI3K-Akt, Wnt, and TGF-β signaling pathways. Together, our results highlighted the sequence and expression characteristics of the piRNAs in the sheep ovary, which will help us understand the roles of piRNAs in the ovine estrus cycle.
Respiratory dysbiosis in cats with spontaneous allergic asthma
Authors: Vientós-Plotts Aida I. , Ericsson Aaron C. , McAdams Zachary L. , Rindt Hansjorg , Reinero Carol R.
Published: September 2022

Deviations from a core airway microbiota have been associated with the development and progression of asthma as well as disease severity. Pet cats represent a large animal model for allergic asthma, as they spontaneously develop a disease similar to atopic childhood asthma. This study aimed to describe the lower airway microbiota of asthmatic pet cats and compare it to healthy cats to document respiratory dysbiosis occurring with airway inflammation. We hypothesized that asthmatic cats would have lower airway dysbiosis characterized by a decrease in richness, diversity, and alterations in microbial community composition including identification of possible pathobionts. In the current study, a significant difference in airway microbiota composition was documented between spontaneously asthmatic pet cats and healthy research cats mirroring the finding of dysbiosis in asthmatic humans.FilobacteriumandAcinetobacterspp. were identified as predominant taxa in asthmatic cats without documented infection based on standard culture and could represent pathobionts in the lower airways of cats.Mycoplasma felis, a known lower airway pathogen of cats, was identified in 35% of asthmatic but not healthy cats.

This article has been published alongside “Temporal changes of the respiratory microbiota as cats transition from health to experimental acute and chronic allergic asthma” (1).
Adaptive mechanisms in no flow vs. low flow ischemia in equine jejunum epithelium: Different paths to the same destination
Authors: Dengler Franziska , Sternberg Felix , Grages Marei , Kästner Sabine BR , Verhaar Nicole
Published: September 2022

Intestinal ischemia reperfusion injury (IRI) is a frequent complication of equine colic. Several mechanisms may be involved in adaptation of the intestinal epithelium to IRI and might infer therapeutic potential, including hypoxia-inducible factor (HIF) 1α, AMP-activated protein kinase (AMPK), nuclear factor-erythroid 2-related factor 2 (NRF2), and induction of autophagy. However, the mechanisms supporting adaptation and thus cellular survival are not completely understood yet. We investigated the activation of specific adaptation mechanisms in both no and low flow ischemia and reperfusion simulated in equine jejunum epitheliumin vivo. We found an activation of HIF1α in no and low flow ischemia as indicated by increased levels of HIF1α target genes and phosphorylation of AMPKα tended to increase during ischemia. Furthermore, the protein expression of the autophagy marker LC3B in combination with decreased expression of nuclear-encoded mitochondrial genes indicates an increased rate of mitophagy in equine intestinal IRI, possibly preventing damage by mitochondria-derived reactive oxygen species (ROS). Interestingly, ROS levels were increased only shortly after the onset of low flow ischemia, which may be explained by an increased antioxidative defense, although NFR2 was not activated in this setup. In conclusion, we could demonstrate that a variety of adaptation mechanisms manipulating different aspects of cellular homeostasis are activated in IRI irrespective of the ischemia model, and that mitophagy might be an important factor for epithelial survival following small intestinal ischemia in horses that should be investigated further.
Neural Adaptive Robust Motion-Tracking Control for Robotic Manipulator Systems
Authors: Galvan-Perez Daniel , Yañez-Badillo Hugo , Beltran-Carbajal Francisco , Rivas-Cambero Ivan , Favela-Contreras Antonio , Tapia-Olvera Ruben
Published: September 2022

This paper deals with the motion trajectory tracking control problem based on output feedback and artificial neural networks for anthropomorphic manipulator robots under disturbed operating scenarios. This class of manipulator robots constitutes nonlinear dynamic systems subjected to disturbance torques induced mainly by work payload. Parametric uncertainty and possible dynamic modeling errors stand for other kind of disturbances that can deteriorate the efficiency and robustness of the tracking of controlled nonlinear robotic system trajectories. In fact, the presence of unknown dynamic disturbances is unavoidable in industrial robotic engineering systems. Therefore, for high-precision applications, such as laser cutting, marking, or welding, effective control schemes should be designed to guarantee adequate motion profile tracking planned on this class of disturbed nonlinear robotic system. In this context, a new adaptive robust motion trajectory tracking control scheme based on output feedback and artificial neural networks of anthropomorphic manipulator robots is presented. Three-layer B-spline artificial neural networks and time-series modeling are properly exploited in the design of novel adaptive robust motion tracking controllers for robotic applications of laser manufacturing. In this way, dependency on detailed nonlinear mathematical modeling of robotic systems is considerably reduced, and real-time estimation of uncertain dynamic disturbances is not required. Furthermore, several cases studies to demonstrate the motion planning tracking control robustness for a class of MIMO nonlinear robotic systems are described. blue Insights for the extension of the introduced output-feedback adaptive neural control design approach for other architecture of nonlinear robotic systems are depicted.