bluuebunny/crossref_metadata_2025 huggingface dataset:
The Balanced Scorecard: Fashion or Virus?
Authors: Madsen Dag , Slåtten Kåre
Published: June 2015

The Balanced Scorecard (BSC) is a one of the most popular and contagious management ideas of our time. In previous research the diffusion and institutionalization of the BSC has been viewed through different theoretical lenses, most notably the management fashion perspective. Recently the virus perspective has been introduced as an alternative theory of how management ideas spread, but so far no study has applied this theory in the context of the BSC. In this paper we show that the fashion and virus perspectives provide complementary insights into the diffusion and institutionalization of the BSC. The fashion perspective is particularly well suited for explaining the infectiousness of the BSC and the ways in which organizations are exposed to the BSC idea. The virus theory can better explain how the BSC idea is implemented as a practice in organizations, and the different trajectories that the BSC idea may take in different contexts. A combination of these two perspectives provides a fuller picture of the diffusion and institutionalization of the BSC.
Development of UAS Design Based on Wideband Antenna Architecture
Authors: Drummond Franklin , Huff Gregory
Published: June 2015

An Unmanned Aerial System (UAS) has been developed which is based on an aerodynamically functionalized planar wideband antenna. The antenna utilizes a planar circular dipole metallization scheme. The aerodynamic structure implements a planform similar to the Nutball flier, a hobbyist flight architecture. The resulting codesign achieved a large impedance bandwidth defined by a voltage standing wave ratio (VSWR) less than 2 from 100 MHz to over 2 GHz and omnidirectional dipole-like radiation patterns at the lower frequency region and more directional patterns at higher frequencies.
Reverse Signaling Contributes to Control of Chronic Inflammation by Anti-TNF Therapeutics
Authors: Sipos Orsolya , Török Annamária , Kalic Tanja , Duda Ernő , Filkor Kata
Published: June 2015

Anti-tumor necrosis factor (TNF) monoclonal antibodies and TNF receptor ectodomain fusion protein are in clinical use to neutralize circulating TNF and ameliorate symptoms of many autoimmune diseases and pathological conditions with chronic inflammation. In this paper we present data to prove that reverse signaling, elicited by agonist molecules interacting with the membrane-bound TNF of myeloid cells, significantly contributes to the therapeutic effect of these anti-TNF medicines. Interaction of agonist monoclonals with cell surface TNF significantly attenuates the expression of pro-inflammatory cytokines and induces changes in the production of extracellular and intracellular signaling molecules. This phenomenon is not dependent on the Fc portion of antibodies as Fab constructs are as efficient as full antibody molecules.
Hypoxia, Oxidative Stress and Fat
Authors: Netzer Nikolaus , Gatterer Hannes , Faulhaber Martin , Burtscher Martin , Pramsohler Stephan , Pesta Dominik
Published: June 2015

Metabolic disturbances in white adipose tissue in obese individuals contribute to the pathogenesis of insulin resistance and the development of type 2 diabetes mellitus. Impaired insulin action in adipocytes is associated with elevated lipolysis and increased free fatty acids leading to ectopic fat deposition in liver and skeletal muscle. Chronic adipose tissue hypoxia has been suggested to be part of pathomechanisms causing dysfunction of adipocytes. Hypoxia can provoke oxidative stress in human and animal adipocytes and reduce the production of beneficial adipokines, such as adiponectin. However, time-dose responses to hypoxia relativize the effects of hypoxic stress. Long-term exposure of fat cells to hypoxia can lead to the production of beneficial substances such as leptin. Knowledge of time-dose responses of hypoxia on white adipose tissue and the time course of generation of oxidative stress in adipocytes is still scarce. This paper reviews the potential links between adipose tissue hypoxia, oxidative stress, mitochondrial dysfunction, and low-grade inflammation caused by adipocyte hypertrophy, macrophage infiltration and production of inflammatory mediators.
Introduction to Oxidative Stress in Biomedical and Biological Research
Authors: Breitenbach Michael , Eckl Peter
Published: June 2015

Oxidative stress is now a well-researched area with thousands of new articles appearing every year. We want to give the reader here an overview of the topics in biomedical and basic oxidative stress research which are covered by the authors of this thematic issue. We also want to give the newcomer a short introduction into some of the basic concepts, definitions and analytical procedures used in this field.
Biodiversity Loss and the Ecological Footprint of Trade
Authors: Lazarus Elias , Lin David , Martindill Jon , Hardiman Jeanette , Pitney Louisa , Galli Alessandro
Published: June 2015

Human pressure on ecosystems is among the major drivers of biodiversity loss. As biodiversity plays a key role in supporting the human enterprise, its decline puts the well-being of human societies at risk. Halting biodiversity loss is therefore a key policy priority, as reflected in the 2020 Aichi Biodiversity Targets under strategic goal A. The Ecological Footprint has become a widely used metric for natural capital and ecosystem accounting, and is frequently cited in the sustainability debate, where it is often used for tracking human-induced pressures on ecosystems and biodiversity. Given its potential role as an indirect metric for biodiversity-related policies, this paper breaks down the Ecological Footprint into its components and analyzes resource and ecosystem service flows at an international level. We discuss its usefulness in tracking the underlying drivers of habitat impacts and biodiversity loss. We find that: China is a major net importer of all biomass biocapacity components; the largest net exporters of forest biocapacity are not low-income countries; a very high proportion of the Ecological Footprint of fishing grounds is traded internationally; Singapore and at least three Middle East countries are almost wholly reliant on net imports for the cropland biocapacity they consume.
Learning a Flexible K-Dependence Bayesian Classifier from the Chain Rule of Joint Probability Distribution
Authors: Wang Limin , Zhao Haoyu
Published: June 2015

As one of the most common types of graphical models, the Bayesian classifier has become an extremely popular approach to dealing with uncertainty and complexity. The scoring functions once proposed and widely used for a Bayesian network are not appropriate for a Bayesian classifier, in which class variable C is considered as a distinguished one. In this paper, we aim to clarify the working mechanism of Bayesian classifiers from the perspective of the chain rule of joint probability distribution. By establishing the mapping relationship between conditional probability distribution and mutual information, a new scoring function, Sum_MI, is derived and applied to evaluate the rationality of the Bayesian classifiers. To achieve global optimization and high dependence representation, the proposed learning algorithm, the flexible K-dependence Bayesian (FKDB) classifier, applies greedy search to extract more information from the K-dependence network structure. Meanwhile, during the learning procedure, the optimal attribute order is determined dynamically, rather than rigidly. In the experimental study, functional dependency analysis is used to improve model interpretability when the structure complexity is restricted.
General Hyperplane Prior Distributions Based on Geometric Invariances for Bayesian Multivariate Linear Regression
Authors: Von Toussaint Udo
Published: June 2015

Based on geometric invariance properties, we derive an explicit prior distribution for the parameters of multivariate linear regression problems in the absence of further prior information. The problem is formulated as a rotationally-invariant distribution of \(L\)-dimensional hyperplanes in \(N\) dimensions, and the associated system of partial differential equations is solved. The derived prior distribution generalizes the already known special cases, e.g., 2D plane in three dimensions.
Understanding Cultural Geography as a Pseudo-Diffusion Process: The Case of the Veneto Region
Authors: Ferilli Guido , Sacco Pier , Buscema Massimo , Blessi Giorgio
Published: June 2015

In this paper, we study the cultural geography of the Veneto Region on the basis of a pseudo-diffusion approach to the analysis of the inherent semantic spatial data. We find somewhat surprising results, and, in particular, that Venice, indisputably the Region’s cultural hub in terms of concentration of activities and facilities, global visibility and attraction of resources, plays a marginal role in determining the momentum of cultural initiative at the regional level as of 2007 data. The areas with the greater momentum are relatively marginal ones but characterized by a strong presence of design-oriented companies that are actively engaging in culture-driven innovation in a context of gradually horizontally-integrated clusters. Our findings call for a revision of the traditional policy approaches that identify centralities in terms of concentration of activities and facilities based on past dynamics, and to design policies accordingly. We argue in favour of a more forward-looking, evidence-based approach.
Investigating Gender and Racial/Ethnic Invariance in Use of a Course Management System in Higher Education
Authors: Li Yi , Wang Qiu , Campbell John
Published: June 2015

This study focused on learning equity in colleges and universities where teaching and learning depends heavily on computer technologies. The study used the Structural Equation Modeling (SEM) to investigate gender and racial/ethnic heterogeneity in the use of a computer based course management system (CMS). Two latent variables (CMS usage and scholastic aptitudes)—with two moderation covariates (gender and ethnicity)—were used to explore their associational relationships with students’ final grades. More than 990 students’ CMS data were collected from courses at a Midwest public university in the United States. The final model indicated that there was gender and racial/ethnic invariance in the use of the CMS. Additionally, CMS use was significantly positively associated with students’ academic achievement. These findings have policy and practical implications for understanding the correlation between technology use and academic achievement in colleges and universities. This study also pointed out future research directions for technology use in higher education.
Dehydriding Process and Hydrogen–Deuterium Exchange of LiBH4–Mg2FeD6 Composites
Authors: Li Guanqiao , Matsuo Motoaki , Aoki Katsutoshi , Ikeshoji Tamio , Orimo Shin-ichi
Published: June 2015

The dehydriding process and hydrogen–deuterium exchange (H–D exchange) of xLiBH4 + (1 − x)Mg2FeD6 (x = 0.25, 0.75) composites has been studied in detail. For the composition with x = 0.25, only one overlapping mass peak of all hydrogen and deuterium related species was observed in mass spectrometry. This implied the simultaneous dehydriding of LiBH4 and Mg2FeD6, despite an almost 190 °C difference in the dehydriding temperatures of the respective discrete complex hydrides. In situ infrared spectroscopy measurements indicated that H–D exchange between [BH4]− and [FeD6]4− had occurred during ball-milling and was promoted upon heating. The extent of H–D exchange was estimated from the areas of the relevant mass signals: immediately prior to the dehydriding, more than two H atoms in [BH4]− was replaced by D atoms. For x = 0.75, H–D exchange also occurred and about one to two H atoms in [BH4]− was replaced by D atoms immediately before the dehydriding. In contrast to the situation for x = 0.25, firstly LiBH4 and Mg2FeD6 dehydrided simultaneously with a special molar ratio = 1:1 at x = 0.75, and then the remaining LiBH4 reacted with the Mg and Fe derived from the dehydriding of Mg2FeD6.
What Is the Relationship between Outdoor Time and Physical Activity, Sedentary Behaviour, and Physical Fitness in Children? A Systematic Review
Authors: Gray Casey , Gibbons Rebecca , Larouche Richard , Sandseter Ellen , Bienenstock Adam , Brussoni Mariana , Chabot Guylaine , Herrington Susan , Janssen Ian , Pickett William , Power Marlene , Stanger Nick , Sampson Margaret , Tremblay Mark
Published: June 2015

The objective of this systematic review was to examine the relationship between outdoor time and: (1) physical activity, (2) cardiorespiratory fitness, (3) musculoskeletal fitness, (4) sedentary behaviour; or (5) motor skill development in children aged 3–12 years. We identified 28 relevant studies that were assessed for quality using the GRADE framework. The systematic review revealed overall positive effects of outdoor time on physical activity, sedentary behaviour, and cardiorespiratory fitness, although causality could not be assumed due to a lack of RCTs. Motor skill development was unrelated to outdoor time; however, this relationship was only examined in a single study of preschool children. No studies were found that examined associations between outdoor time and musculoskeletal fitness.
Position Statement on Active Outdoor Play
Authors: Tremblay Mark , Gray Casey , Babcock Shawna , Barnes Joel , Bradstreet Christa , Carr Dawn , Chabot Guylaine , Choquette Louise , Chorney David , Collyer Cam , Herrington Susan , Janson Katherine , Janssen Ian , Larouche Richard , Pickett William , Power Marlene , Sandseter Ellen , Simon Brenda , Brussoni Mariana
Published: June 2015

A diverse, cross-sectorial group of partners, stakeholders and researchers, collaborated to develop an evidence-informed Position Statement on active outdoor play for children aged 3–12 years. The Position Statement was created in response to practitioner, academic, legal, insurance and public debate, dialogue and disagreement on the relative benefits and harms of active (including risky) outdoor play. The Position Statement development process was informed by two systematic reviews, a critical appraisal of the current literature and existing position statements, engagement of research experts (N = 9) and cross-sectorial individuals/organizations (N = 17), and an extensive stakeholder consultation process (N = 1908). More than 95% of the stakeholders consulted strongly agreed or somewhat agreed with the Position Statement; 14/17 participating individuals/organizations endorsed it; and over 1000 additional individuals and organizations requested their name be listed as a supporter. The final Position Statement on Active Outdoor Play states: “Access to active play in nature and outdoors—with its risks— is essential for healthy child development. We recommend increasing children’s opportunities for self-directed play outdoors in all settings—at home, at school, in child care, the community and nature.” The full Position Statement provides context for the statement, evidence supporting it, and a series of recommendations to increase active outdoor play opportunities to promote healthy child development.
The Impact of Different Degrees of Feedback on Physical Activity Levels: A 4-Week Intervention Study
Authors: Van Hoye Karen , Boen Filip , Lefevre Johan
Published: June 2015

Assessing levels of physical activity (PA) and providing feedback about these levels might have an effect on participant’s PA behavior. This study discusses the effect of different levels of feedback—from minimal to use of a feedback display and coach—on PA over a 4-week intervention period. PA was measured at baseline, during and immediately after the intervention. Participants (n = 227) were randomly assigned to a Minimal Intervention Group (MIG-no feedback), Pedometer Group (PG-feedback on steps taken), Display Group (DG-feedback on steps, minutes of moderate to vigorous physical activity and energy expenditure) or Coaching Group (CoachG-same as DG with need-supportive coaching). Two-way ANCOVA showed no significant Group × Time interaction effect for the different PA variables between the MIG and PG. Also no differences emerged between PG and DG. As hypothesized, CoachG had higher PA values throughout the intervention compared with DG. Self-monitoring using a pedometer resulted in more steps compared with a no-feedback condition at the start of the intervention. However, adding individualized coaching seems necessary to increase the PA level until the end of the intervention.
Predicting the Probability of Failure of Cementitious Sewer Pipes Using Stochastic Finite Element Method
Authors: Alani Amir , Faramarzi Asaad
Published: June 2015

In this paper, a stochastic finite element method (SFEM) is employed to investigate the probability of failure of cementitious buried sewer pipes subjected to combined effect of corrosion and stresses. A non-linear time-dependant model is used to determine the extent of concrete corrosion. Using the SFEM, the effects of different random variables, including loads, pipe material, and corrosion on the remaining safe life of the cementitious sewer pipes are explored. A numerical example is presented to demonstrate the merit of the proposed SFEM in evaluating the effects of the contributing parameters upon the probability of failure of cementitious sewer pipes. The developed SFEM offers many advantages over traditional probabilistic techniques since it does not use any empirical equations in order to determine failure of pipes. The results of the SFEM can help the concerning industry (e.g., water companies) to better plan their resources by providing accurate prediction for the remaining safe life of cementitious sewer pipes.
Quantitative Structure-Antioxidant Activity Models of Isoflavonoids: A Theoretical Study
Authors: Castellano Gloria , Torrens Francisco
Published: June 2015

Seventeen isoflavonoids from isoflavone, isoflavanone and isoflavan classes are selected from Dalbergia parviflora. The ChEMBL database is representative from these molecules, most of which result highly drug-like. Binary rules appear risky for the selection of compounds with high antioxidant capacity in complementary xanthine/xanthine oxidase, ORAC, and DPPH model assays. Isoflavonoid structure-activity analysis shows the most important properties (log P, log D, pKa, QED, PSA, NH + OH ≈ HBD, N + O ≈ HBA). Some descriptors (PSA, HBD) are detected as more important than others (size measure Mw, HBA). Linear and nonlinear models of antioxidant potency are obtained. Weak nonlinear relationships appear between log P, etc. and antioxidant activity. The different capacity trends for the three complementary assays are explained. Isoflavonoids potency depends on the chemical form that determines their solubility. Results from isoflavonoids analysis will be useful for activity prediction of new sets of flavones and to design drugs with antioxidant capacity, which will prove beneficial for health with implications for antiageing therapy.
The Effect of Electrospun Gelatin Fibers Alignment on Schwann Cell and Axon Behavior and Organization in the Perspective of Artificial Nerve Design
Authors: Gnavi Sara , Fornasari Benedetta , Tonda-Turo Chiara , Laurano Rossella , Zanetti Marco , Ciardelli Gianluca , Geuna Stefano
Published: June 2015

Electrospun fibrous substrates mimicking extracellular matrices can be prepared by electrospinning, yielding aligned fibrous matrices as internal fillers to manufacture artificial nerves. Gelatin aligned nano-fibers were prepared by electrospinning after tuning the collector rotation speed. The effect of alignment on cell adhesion and proliferation was tested in vitro using primary cultures, the Schwann cell line, RT4-D6P2T, and the sensory neuron-like cell line, 50B11. Cell adhesion and proliferation were assessed by quantifying at several time-points. Aligned nano-fibers reduced adhesion and proliferation rate compared with random fibers. Schwann cell morphology and organization were investigated by immunostaining of the cytoskeleton. Cells were elongated with their longitudinal body parallel to the aligned fibers. B5011 neuron-like cells were aligned and had parallel axon growth when cultured on the aligned gelatin fibers. The data show that the alignment of electrospun gelatin fibers can modulate Schwann cells and axon organization in vitro, suggesting that this substrate shows promise as an internal filler for the design of artificial nerves for peripheral nerve reconstruction.
A Structural Overview of RNA-Dependent RNA Polymerases from the Flaviviridae Family
Authors: Wu Jiqin , Liu Weichi , Gong Peng
Published: June 2015

RNA-dependent RNA polymerases (RdRPs) from the Flaviviridae family are representatives of viral polymerases that carry out RNA synthesis through a de novo initiation mechanism. They share a ≈ 600-residue polymerase core that displays a canonical viral RdRP architecture resembling an encircled right hand with palm, fingers, and thumb domains surrounding the active site. Polymerase catalytic motifs A–E in the palm and motifs F/G in the fingers are shared by all viral RdRPs with sequence and/or structural conservations regardless of the mechanism of initiation. Different from RdRPs carrying out primer-dependent initiation, Flaviviridae and other de novo RdRPs utilize a priming element often integrated in the thumb domain to facilitate primer-independent initiation. Upon the transition to the elongation phase, this priming element needs to undergo currently unresolved conformational rearrangements to accommodate the growth of the template-product RNA duplex. In the genera of Flavivirus and Pestivirus, the polymerase module in the C-terminal part of the RdRP protein may be regulated in cis by the N-terminal region of the same polypeptide. Either being a methyltransferase in Flavivirus or a functionally unclarified module in Pestivirus, this region could play auxiliary roles for the canonical folding and/or the catalysis of the polymerase, through defined intra-molecular interactions.
The N-Acetylglutamate Synthase Family: Structures, Function and Mechanisms
Authors: Shi Dashuang , Allewell Norma , Tuchman Mendel
Published: June 2015

N-acetylglutamate synthase (NAGS) catalyzes the production of N-acetylglutamate (NAG) from acetyl-CoA and l-glutamate. In microorganisms and plants, the enzyme functions in the arginine biosynthetic pathway, while in mammals, its major role is to produce the essential co-factor of carbamoyl phosphate synthetase 1 (CPS1) in the urea cycle. Recent work has shown that several different genes encode enzymes that can catalyze NAG formation. A bifunctional enzyme was identified in certain bacteria, which catalyzes both NAGS and N-acetylglutamate kinase (NAGK) activities, the first two steps of the arginine biosynthetic pathway. Interestingly, these bifunctional enzymes have higher sequence similarity to vertebrate NAGS than those of the classical (mono-functional) bacterial NAGS. Solving the structures for both classical bacterial NAGS and bifunctional vertebrate-like NAGS/K has advanced our insight into the regulation and catalytic mechanisms of NAGS, and the evolutionary relationship between the two NAGS groups.
Synthesis, DNA Binding, and Antiproliferative Activity of Novel Acridine-Thiosemicarbazone Derivatives
Authors: De Almeida Sinara , Lafayette Elizabeth , Da Silva Lúcia , Amorim Cézar , De Oliveira Tiago , Ruiz Ana , De Carvalho João , De Moura Ricardo , Beltrão Eduardo , De Lima Maria , Júnior Luiz
Published: June 2015

In this work, the acridine nucleus was used as a lead-compound for structural modification by adding different substituted thiosemicarbazide moieties. Eight new (Z)-2-(acridin-9-ylmethylene)-N-phenylhydrazinecarbothioamide derivatives (3a–h) were synthesized, their antiproliferative activities were evaluated, and DNA binding properties were performed with calf thymus DNA (ctDNA) by electronic absorption and fluorescence spectroscopies. Both hyperchromic and hypochromic effects, as well as red or blue shifts were demonstrated by addition of ctDNA to the derivatives. The calculated binding constants ranged from 1.74 × 104 to 1.0 × 106 M−1 and quenching constants from −0.2 × 104 to 2.18 × 104 M−1 indicating high affinity to ctDNA base pairs. The most efficient compound in binding to ctDNA in vitro was (Z)-2-(acridin-9-ylmethylene)-N- (4-chlorophenyl) hydrazinecarbothioamide (3f), while the most active compound in antiproliferative assay was (Z)-2-(acridin-9-ylmethylene)-N-phenylhydrazinecarbothioamide (3a). There was no correlation between DNA-binding and in vitro antiproliferative activity, but the results suggest that DNA binding can be involved in the biological activity mechanism. This study may guide the choice of the size and shape of the intercalating part of the ligand and the strategic selection of substituents that increase DNA-binding or antiproliferative properties.
Roles of B Cell-Intrinsic TLR Signals in Systemic Lupus Erythematosus
Authors: Ma Kongyang , Li Jingyi , Fang Yongfei , Lu Liwei
Published: June 2015

Toll-like receptors (TLRs) are a large family of pattern recognition receptors. TLR signals are involved in the pathogenesis of systemic lupus erythematosus. Mouse and human B cells constitutively express most TLRs. Many B cell subpopulations are highly responsive to certain TLR ligation, including B-1 B cells, transitional B cells, marginal zone B cells, germinal center B cell and memory B cells. The B cell-intrinsic TLR signals play critical roles during lupus process. In this review, roles of B cell-intrinsic TLR2, 4, 7, 8 and 9 signals are discussed during lupus pathogenesis in both mouse model and patients. Moreover, mechanisms underlying TLR ligation-triggered B cell activation and signaling pathways are highlighted.
A New Treatment Strategy for Inactivating Algae in Ballast Water Based on Multi-Trial Injections of Chlorine
Authors: Sun Jinyang , Wang Junsheng , Pan Xinxiang , Yuan Haichao
Published: June 2015

Ships’ ballast water can carry aquatic organisms into foreign ecosystems. In our previous studies, a concept using ion exchange membrane electrolysis to treat ballast water has been proven. In addition to other substantial approaches, a new strategy for inactivating algae is proposed based on the developed ballast water treatment system. In the new strategy, the means of multi-trial injection with small doses of electrolytic products is applied for inactivating algae. To demonstrate the performance of the new strategy, contrast experiments between new strategies and routine processes were conducted. Four algae species including Chlorella vulgaris, Platymonas subcordiformis, Prorocentrum micans and Karenia mikimotoi were chosen as samples. The different experimental parameters are studied including the injection times and doses of electrolytic products. Compared with the conventional one trial injection method, mortality rate time (MRT) and available chlorine concentration can be saved up to about 84% and 40%, respectively, under the application of the new strategy. The proposed new approach has great potential in practical ballast water treatment. Furthermore, the strategy is also helpful for deep insight of mechanism of algal tolerance.
Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments
Authors: Cui Yanhua , Hu Tong , Qu Xiaojun , Zhang Lanwei , Ding Zhongqing , Dong Aijun
Published: June 2015

Plasmids are widely distributed in different sources of lactic acid bacteria (LAB) as self-replicating extrachromosomal genetic materials, and have received considerable attention due to their close relationship with many important functions as well as some industrially relevant characteristics of the LAB species. They are interesting with regard to the development of food-grade cloning vectors. This review summarizes new developments in the area of lactic acid bacteria plasmids and aims to provide up to date information that can be used in related future research.
Broader Autism Phenotype in Siblings of Children with ASD—A Review
Authors: Pisula Ewa , Ziegart-Sadowska Karolina
Published: June 2015

Although less pronounced, social, cognitive, and personality characteristics associated with autism spectrum disorders (ASD) may be present in people who do not meet ASD diagnostic criteria, especially in first-degree relatives of individuals with ASD. Research on these characteristics, referred to as broader autism phenotype (BAP), provides valuable data on potential expressions of autism-specific deficits in the context of family relations. This paper offers a review of research on BAP in siblings of individuals with ASD, focusing on reports regarding social, communication, and cognitive deficits, published from 1993 to 2014. The studies are divided into two groups based on participants’ age: papers on preschool and older siblings of individuals with ASD; and publications on infants at risk for ASD. On the basis of this review, suggestions are offered for further research and its significance for our understanding of the genetic determinants of autism.
Identification of Eastern United States Reticulitermes Termite Species via PCR-RFLP, Assessed Using Training and Test Data
Authors: Garrick Ryan , Collins Benjamin , Yi Rachel , Dyer Rodney , Hyseni Chaz
Published: June 2015

Reticulitermes termites play key roles in dead wood decomposition and nutrient cycling in forests. They also damage man-made structures, resulting in considerable economic loss. In the eastern United States, five species (R. flavipes, R. virginicus, R. nelsonae, R. hageni and R. malletei) have overlapping ranges and are difficult to distinguish morphologically. Here we present a molecular tool for species identification. It is based on polymerase chain reaction (PCR) amplification of a section of the mitochondrial cytochrome oxidase subunit II gene, followed by a three-enzyme restriction fragment length polymorphism (RFLP) assay, with banding patterns resolved via agarose gel electrophoresis. The assay was designed using a large set of training data obtained from a public DNA sequence database, then evaluated using an independent test panel of Reticulitermes from the Southern Appalachian Mountains, for which species assignments were determined via phylogenetic comparison to reference sequences. After refining the interpretive framework, the PCR-RFLP assay was shown to provide accurate identification of four co-occurring species (the fifth species, R. hageni, was absent from the test panel, so accuracy cannot yet be extended to training data). The assay is cost- and time-efficient, and will help improve knowledge of Reticulitermes species distributions.
Association between Osteopontin Promoter Gene Polymorphisms and Haplotypes with Risk of Diabetic Nephropathy
Authors: Cheema Balneek , Iyengar Sreenivasa , Sharma Rajni , Kohli Harbir , Bhansali Anil , Khullar Madhu
Published: June 2015

Background: Osteopontin (OPN) C-443T promoter polymorphism has been shown as a genetic risk factor for diabetic nephropathy (DN) in type 2 diabetic patients (T2D). Methods: In the present study we investigated the association of three functional promoter gene polymorphisms C-443T, delG-156G, and G-66T and their haplotypes with the risk of DN and estimated Glomerular Filtration Rate (eGFR) in Asian Indians T2D patients using Real time PCR based Taqman assay. A total of 1165 T2D patients, belonging to two independently ascertained Indian Asian cohorts, were genotyped for three OPN promoter polymorphisms C-443T (rs11730582), delG-156G (rs17524488) and G-66T (rs28357094). Results: -156G allele and GG genotypes (delG-156G) and haplotypes G-C-G and T-C-G (G-66T, C-443T, delG-156G) were associated with decreased risk of DN and higher eGFR. Haplotype G-T-delG and T-T-delG (G-66T, C-443T, delG-156G) were identified as risk haplotypes, as shown by lower eGFR. Conclusion: This is the first study to report an association of OPN promoter gene polymorphisms; G-66T and delG-156G and their haplotypes with DN in T2D. Our results suggest an association between OPN promoter gene polymorphisms and their haplotypes with DN.
Nanocomposites Based on Thermoplastic Polymers and Functional Nanofiller for Sensor Applications
Authors: Coiai Serena , Passaglia Elisa , Pucci Andrea , Ruggeri Giacomo
Published: June 2015

Thermoplastic polymers like polyolefins, polyesters, polyamide, and styrene polymers are the most representative commodity plastics thanks to their cost-efficient manufacturing processes, excellent thermomechanical properties and their good environmental compatibility, including easy recycling. In the last few decades much effort has been devoted worldwide to extend the applications of such materials by conferring on them new properties through mixing and blending with different additives. In this latter context, nanocomposites have recently offered new exciting possibilities. This review discusses the successful use of nanostructured dispersed substrates in designing new stimuli-responsive nanocomposites; in particular, it provides an updated description of the synthetic routes to prepare nanostructured systems having the typical properties of thermoplastic polymers (continuous matrix), but showing enhanced optical, conductive, and thermal features dependent on the dispersion topology. The controlled nanodispersion of functional labeled clays, noble metal nanoparticles and carbon nanotubes is here evidenced to play a key role in producing hybrid thermoplastic materials that have been used in the design of devices, such as NLO devices, chemiresistors, temperature and deformation sensors.
Fractional Slot Concentrated Windings: A New Method to Manage the Mutual Inductance between Phases in Three-Phase Electrical Machines and Multi-Star Electrical Machines
Authors: Barre Olivier , Napame Bellemain
Published: June 2015

Mutual inductance is a phenomenon caused by the circulation of the magnetic flux in the core of an electrical machine. It is the result of the effect of the current flowing in one phase on the other phases. In conventional three-phase machines, such an effect has no influence on the electrical behaviour of the device. Although these machines are powered by power inverters, no problem should occur. The result is not the same for multi-star machines. If these machines are using a conventional winding structure, namely distributed windings, and are powered by voltage source converters, current ripples appear in the power supply lines. These current ripples are related to magnetic couplings between the stars. Designers should check these current ripples in order to stay within the limits imposed by the specifications. These electric current disturbances also provide torque ripples. With concentrated windings, a new degree of freedom appears; the configuration—number of slots/number of poles—can have a positive impact. The circulation of the magnetic flux is the initial phenomenon that produces the mutual inductance. The main goal of this discussion is to describe a design method that is able to produce not only a machine with low mutual inductance between phases, but also a multi-star machine where the stars and the phases are magnetically decoupled or less coupled. This discussion only takes into account the machines that use permanent magnets mounted on the rotor surface. This article is part of a study aimed at designing a high efficiency generator using fractional-slot concentrated-windings (FSCW).
The Complement of Binary Klein Quadric as a Combinatorial Grassmannian
Authors: Saniga Metod
Published: June 2015

Given a hyperbolic quadric of PG(5, 2), there are 28 points off this quadric and 56 lines skew to it. It is shown that the (286; 563)-configuration formed by these points and lines is isomorphic to the combinatorial Grassmannian of type G2(8). It is also pointed out that a set of seven points of G2(8) whose labels share a mark corresponds to a Conwell heptad of PG(5, 2). Gradual removal of Conwell heptads from the (286; 563)-configuration yields a nested sequence of binomial configurations identical with part of that found to be associated with Cayley-Dickson algebras (arXiv:1405.6888).
Use of Iron Powder to Obtain High Yields of Leptothrix Sheaths in Culture
Authors: Suzuki Tomoko , Kunoh Tatsuki , Nakatsuka Daisuke , Hashimoto Hideki , Tamura Katsunori , Kunoh Hitoshi , Takada Jun
Published: June 2015

The Leptothrix species, Fe-oxidizing bacteria, produce an extracellular, microtubular sheath with a complicated organic–inorganic hybrid nature. We have discovered diverse industrial functions for this material, e.g., electrode material for Li-ion batteries, catalyst enhancers, pigments, plant growth promoters, and plant protectants. To consistently obtain material with the qualitative and quantitative stability needed for industrial applications, we focused on developing an optimum culture system for sheath synthesis by the Leptothrix sp. strain OUMS1. Although we have used Fe plates as an Fe source in the liquid silicon-glucose-peptone medium (SGP), the plates do not yield a consistent quality or precise mass, and formation of Fe-encrusted sheath is restricted to a surface of the plates, which limits harvest yield. In this study, to obtain a high yield of sheaths, we cultured OUMS1 in SGP supplemented with Fe powders. The addition of Fe powders to the medium (up to 14.0 g/L) did not adversely influence growth of OUMS1. The final yield of sheaths was about 10-fold greater than in the Fe plate culture. The sheaths also maintained a microtubular form and crystalline texture similar to those produced on Fe plates in SGP. The results proved the usefulness of Fe powder for consistently high yields of Fe-encrusted sheaths of stable quality.
Role and Regulation of Glutathione Metabolism in Plasmodium falciparum
Authors: Müller Sylke
Published: June 2015

Malaria in humans is caused by one of five species of obligate intracellular protozoan parasites of the genus Plasmodium. P. falciparum causes the most severe disease and is responsible for 600,000 deaths annually, primarily in Sub-Saharan Africa. It has long been suggested that during their development, malaria parasites are exposed to environmental and metabolic stresses. One strategy to drug discovery was to increase these stresses by interfering with the parasites’ antioxidant and redox systems, which may be a valuable approach to disease intervention. Plasmodium possesses two redox systems—the thioredoxin and the glutathione system—with overlapping but also distinct functions. Glutathione is the most abundant low molecular weight redox active thiol in the parasites existing primarily in its reduced form representing an excellent thiol redox buffer. This allows for an efficient maintenance of the intracellular reducing environment of the parasite cytoplasm and its organelles. This review will highlight the mechanisms that are responsible for sustaining an adequate concentration of glutathione and maintaining its redox state in Plasmodium. It will provide a summary of the functions of the tripeptide and will discuss the potential of glutathione metabolism for drug discovery against human malaria parasites.
Integrated LC-MS/MS Analytical Systems and Physical Inspection for the Analysis of a Botanical Herbal Preparation
Authors: Lai Kuan-Ming , Cheng Yung-Yi , Tsai Tung-Hu
Published: June 2015

The herbal decoction process is generally inconvenient and unpleasant. To avoid using herbal medicine decoctions, various high-quality industrial and pharmaceutical herbal decoction products have been used in clinical applications for more than ten years in Taiwan. However, the consistency and standardization of the quality of these herbal medicines are goals that remain to be achieved. The aim of study was to develop a validated liquid chromatography-tandem electrospray ionization mass spectrometry (LC-MS/MS) method to determine the biomarkers astragaloside I, astragaloside IV, formononetin, cinnamic acid, paeoniflorin and gingerol in the herbal preparation known as Huangqi-Guizhi-Wuwu (HGW). To investigate the physical quality of HGW, methods such as scanning electron microscopy, light microscopy with Congo red and potassium iodine staining, solubility measurements, swelling power tests, and crude fiber analysis were used to identify additives in commercial pharmaceutical products. The optimal LC-MS/MS multiple reaction-monitoring system included a gradient program using 5 mM ammonium acetate buffer with 0.05% formic acid/methanol. The results demonstrate deviations in biomarker content across different brands. In addition to the herbal extract, starch and excipients in the pharmaceutical granule, and crushed crude herb powder was added to the pharmaceutical products to increase their herbal ingredient content. In conclusion, a rigorous examination should be performed to certify the quality of the herbal products.
Computational Studies of Benzoxazinone Derivatives as Antiviral Agents against Herpes Virus Type 1 Protease
Authors: Mello Juliana , Botelho Nathália , Souza Alessandra , Oliveira Riethe , Brito Monique , Abrahim-Vieira Bárbara , Sodero Ana , Castro Helena , Cabral Lucio , Miceli Leonardo , Rodrigues Carlos
Published: June 2015

Herpes simplex virus infections have been described in the medical literature for centuries, yet the the drugs available nowadays for therapy are largely ineffective and low oral bioavailability plays an important role on the inefficacy of the treatments. Additionally, the details of the inhibition of Herpes Virus type 1 are still not fully understood. Studies have shown that several viruses encode one or more proteases required for the production new infectious virions. This study presents an analysis of the interactions between HSV-1 protease and benzoxazinone derivatives through a combination of structure-activity relationships, comparative modeling and molecular docking studies. The structure activity relationship results showed an important contribution of hydrophobic and polarizable groups and limitations for bulky groups in specific positions. Two Herpes Virus type 1 protease models were constructed and compared to achieve the best model which was obtained by MODELLER. Molecular docking results pointed to an important interaction between the most potent benzoxazinone derivative and Ser129, consistent with previous mechanistic data. Moreover, we also observed hydrophobic interactions that may play an important role in the stabilization of inhibitors in the active site. Finally, we performed druglikeness and drugscore studies of the most potent derivatives and the drugs currently used against Herpes virus.
Transcriptomic Analysis of Flower Blooming in Jasminum sambac through De Novo RNA Sequencing
Authors: Li Yong-Hua , Zhang Wei , Li Yong
Published: June 2015

Flower blooming is a critical and complicated plant developmental process in flowering plants. However, insufficient information is available about the complex network that regulates flower blooming in Jasminum sambac. In this study, we used the RNA-Seq platform to analyze the molecular regulation of flower blooming in J. sambac by comparing the transcript profiles at two flower developmental stages: budding and blooming. A total of 4577 differentially-expressed genes (DEGs) were identified between the two floral stages. The Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses revealed that the DEGs in the “oxidation-reduction process”, “extracellular region”, “steroid biosynthesis”, “glycosphingolipid biosynthesis”, “plant hormone signal transduction” and “pentose and glucuronate interconversions” might be associated with flower development. A total of 103 and 92 unigenes exhibited sequence similarities to the known flower development and floral scent genes from other plants. Among these unigenes, five flower development and 19 floral scent unigenes exhibited at least four-fold differences in expression between the two stages. Our results provide abundant genetic resources for studying the flower blooming mechanisms and molecular breeding of J. sambac.
NF-κBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients
Authors: Camargo Rodolfo , Riccardi Daniela , Ribeiro Henrique , Carnevali Luiz , De Matos-Neto Emidio , Enjiu Lucas , Neves Rodrigo , Lima Joanna , Figuerêdo Raquel , De Alcântara Paulo , Maximiano Linda , Otoch José , Batista Miguel , Püschel Gerhard , Seelaender Marilia
Published: June 2015

Cancer cachexia, of which the most notable symptom is severe and rapid weight loss, is present in the majority of patients with advanced cancer. Inflammatory mediators play an important role in the development of cachexia, envisaged as a chronic inflammatory syndrome. The white adipose tissue (WAT) is one of the first compartments affected in cancer cachexia and suffers a high rate of lipolysis. It secretes several cytokines capable of directly regulating intermediate metabolism. A common pathway in the regulation of the expression of pro-inflammatory cytokines in WAT is the activation of the nuclear transcription factor kappa-B (NF-κB). We have examined the gene expression of the subunits NF-κBp65 and NF-κBp50, as well as NF-κBp65 and NF-κBp50 binding, the gene expression of pro-inflammatory mediators under NF-κB control (IL-1β, IL-6, INF-γ, TNF-α, MCP-1), and its inhibitory protein, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκB-α). The observational study involved 35 patients (control group, n = 12 and cancer group, n = 23, further divided into cachectic and non-cachectic). NF-κBp65 and its target genes expression (TNF-α, IL-1β, MCP-1 and IκB-α) were significantly higher in cachectic cancer patients. Moreover, NF-κBp65 gene expression correlated positively with the expression of its target genes. The results strongly suggest that the NF-κB pathway plays a role in the promotion of WAT inflammation during cachexia.
Opportunistic Premise Plumbing Pathogens: Increasingly Important Pathogens in Drinking Water
Authors: Falkinham Joseph , Pruden Amy , Edwards Marc
Published: June 2015

Opportunistic premise plumbing pathogens are responsible for a significant number of infections whose origin has been traced to drinking water. These opportunistic pathogens represent an emerging water borne disease problem with a major economic cost of at least $1 billion annually. The common features of this group of waterborne pathogens include: disinfectant-resistance, pipe surface adherence and biofilm formation, growth in amoebae, growth on low organic concentrations, and growth at low oxygen levels. Their emergence is due to the fact that conditions resulting from drinking water treatment select for them. As such, there is a need for novel approaches to reduce exposure to these pathogens. In addition to much-needed research, controls to reduce numbers and human exposure can be instituted independently by utilities and homeowners and hospital- and building-operators.