bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation of the Impact of Load on the Magnetic Field Strength of the Crane by the Magnetic Metal Memory Technique
Authors: Koson-Schab Agnieszka , Szpytko Janusz
Published: December 2020

The paper deals with the problem of applicability of the metal magnetic memory (MMM) technique in the crane structural inspection and monitoring. The MMM method does not require the external magnetization of a structure that results in reduction of downtime of maintenance operations. Measurement of the intensity of the self-magnetic leakage signal can be an alternative to other non-destructive methods used for inspection of a large crane’s structure and equipment. However, the complexity of the residual magnetization effect in the MMM technique is the problem with its application. Thus, the magnetic flux leakage behavior on the crane girder surface under different measurements and the crane’s load conditions is analyzed based on the results obtained during experiments carried out on the overhead traveling crane.
An Improved Simulation Model for Pedestrian Crowd Evacuation
Authors: Muhammed Danial A. , Rashid Tarik A. , Alsadoon Abeer , Bacanin Nebojsa , Fattah Polla , Mohammadi Mokhtar , Banerjee Indradip
Published: December 2020

This paper works on one of the most recent pedestrian crowd evacuation models—i.e., “a simulation model for pedestrian crowd evacuation based on various AI techniques”—which was developed in late 2019. This study adds a new feature to the developed model by proposing a new method and integrating it into the model. This method enables the developed model to find a more appropriate evacuation area design regarding safety due to selecting the best exit door location among many suggested locations. This method is completely dependent on the selected model’s output—i.e., the evacuation time for each individual within the evacuation process. The new method finds an average of the evacuees’ evacuation times of each exit door location; then, based on the average evacuation time, it decides which exit door location would be the best exit door to be used for evacuation by the evacuees. To validate the method, various designs for the evacuation area with various written scenarios were used. The results showed that the model with this new method could predict a proper exit door location among many suggested locations. Lastly, from the results of this research using the integration of this newly proposed method, a new capability for the selected model in terms of safety allowed the right decision in selecting the finest design for the evacuation area among other designs.
Some Escape Time Results for General Complex Polynomials and Biomorphs Generation by a New Iteration Process
Authors: Jolaoso Lateef Olakunle , Khan Safeer Hussain
Published: December 2020

Biomorphs are graphic objects with very interesting shapes resembling unicellular and microbial organisms such as bacteria. They have applications in different fields like medical science, art, painting, engineering and the textile industry. In this paper, we present for the first time escape criterion results for general complex polynomials containing quadratic, cubic and higher order polynomials. We do so by using a more general iteration method also used for the first time in this field. This also generalizes some previous results. Then, biomorphs are generated using an algorithm whose pseudocode is included. A visualization of the biomorphs for certain polynomials is presented and their graphical behaviour with respect to variation of parameters is examined.
New Computational Geometry Methods Applied to Solve Complex Problems of Radiative Transfer
Authors: Salguero-Andújar Francisco , Cabeza-Lainez Joseph-Maria
Published: December 2020

Diverse problems of radiative transfer remain as yet unsolved due to the difficulties of the calculations involved, especially if the intervening shapes are geometrically complex. The main goal of our investigation in this domain is to convert the equations that were previously derived into a graphical interface based on the projected solid-angle principle. Such a procedure is now feasible by virtue of several widely diffused programs for Algorithms Aided Design (AAD). Accuracy and reliability of the process is controlled in the basic examples by means of subroutines from the analytical software DianaX, developed at an earlier stage by the authors, though mainly oriented to closed cuboidal or curved volumes. With this innovative approach, the often cumbersome calculation procedure of lighting, thermal or even acoustic energy exchange can be simplified and made available for the neophyte, with the undeniable advantage of reduced computer time.
Time-Consistent Investment and Reinsurance Strategies for Mean-Variance Insurers under Stochastic Interest Rate and Stochastic Volatility
Authors: Zhu Jiaqi , Li Shenghong
Published: December 2020

This paper studies the time-consistent optimal investment and reinsurance problem for mean-variance insurers when considering both stochastic interest rate and stochastic volatility in the financial market. The insurers are allowed to transfer insurance risk by proportional reinsurance or acquiring new business, and the jump-diffusion process models the surplus process. The financial market consists of a risk-free asset, a bond, and a stock modelled by Heston’s stochastic volatility model. Interest rate in the market is modelled by the Vasicek model. By using extended dynamic programming approach, we explicitly derive equilibrium reinsurance-investment strategies and value functions. In addition, we provide and prove a verification theorem and then prove the solution we get satisfies it. Moreover, sensitive analysis is given to show the impact of several model parameters on equilibrium strategy and the efficient frontier.
Structure Elucidation and Functional Studies of a Novel β-hairpin Antimicrobial Peptide from the Marine Polychaeta Capitella teleta
Authors: Panteleev Pavel V. , Tsarev Andrey V. , Safronova Victoria N. , Reznikova Olesia V. , Bolosov Ilia A. , Sychev Sergei V. , Shenkarev Zakhar O. , Ovchinnikova Tatiana V.
Published: December 2020

Endogenous antimicrobial peptides (AMPs) are evolutionary ancient molecular factors of innate immunity that play a key role in host defense. Among the most active and stable under physiological conditions AMPs are the peptides of animal origin that adopt a β-hairpin conformation stabilized by disulfide bridges. In this study, a novel BRICHOS-domain related AMP from the marine polychaeta Capitella teleta, named capitellacin, was produced as the recombinant analogue and investigated. The mature capitellacin exhibits high homology with the known β-hairpin AMP family—tachyplesins and polyphemusins from the horseshoe crabs. The β-hairpin structure of the recombinant capitellacin was proved by CD and NMR spectroscopy. In aqueous solution the peptide exists as monomeric right-handed twisted β-hairpin and its structure does not reveal significant amphipathicity. Moreover, the peptide retains this conformation in membrane environment and incorporates into lipid bilayer. Capitellacin exhibits a strong antimicrobial activity in vitro against a wide panel of bacteria including extensively drug-resistant strains. In contrast to other known β-hairpin AMPs, this peptide acts apparently via non-lytic mechanism at concentrations inhibiting bacterial growth. The molecular mechanism of the peptide antimicrobial action does not seem to be related to the inhibition of bacterial translation therefore other molecular targets may be assumed. The reduced cytotoxicity against human cells and high antibacterial cell selectivity as compared to tachyplesin-1 make it an attractive candidate compound for an anti-infective drug design.
Fishery Wastes as a Yet Undiscovered Treasure from the Sea: Biomolecules Sources, Extraction Methods and Valorization
Authors: Caruso Gabriella , Floris Rosanna , Serangeli Claudio , Di Paola Luisa
Published: December 2020

The search for new biological sources of commercial value is a major goal for the sustainable management of natural resources. The huge amount of fishery by-catch or processing by-products continuously produced needs to be managed to avoid environmental problems and keep resource sustainability. Fishery by-products can represent an interesting source of high added value bioactive compounds, such as proteins, carbohydrates, collagen, polyunsaturated fatty acids, chitin, polyphenolic constituents, carotenoids, vitamins, alkaloids, tocopherols, tocotrienols, toxins; nevertheless, their biotechnological potential is still largely underutilized. Depending on their structural and functional characteristics, marine-derived biomolecules can find several applications in food industry, agriculture, biotechnological (chemical, industrial or environmental) fields. Fish internal organs are a rich and underexplored source of bioactive compounds; the fish gut microbiota biosynthesizes essential or short-chain fatty acids, vitamins, minerals or enzymes and is also a source of probiotic candidates, in turn producing bioactive compounds with antibiotic and biosurfactant/bioemulsifier activities. Chemical, enzymatic and/or microbial processing of fishery by-catch or processing by-products allows the production of different valuable bioactive compounds; to date, however, the lack of cost-effective extraction strategies so far has prevented their exploitation on a large scale. Standardization and optimization of extraction procedures are urgently required, as processing conditions can affect the qualitative and quantitative properties of these biomolecules. Valorization routes for such raw materials can provide a great additional value for companies involved in the field of bioprospecting. The present review aims at collecting current knowledge on fishery by-catch or by-products, exploring the valorization of their active biomolecules, in application of the circular economy paradigm applied to the fishery field. It will address specific issues from a biorefinery perspective: (i) fish tissues and organs as potential sources of metabolites, antibiotics and probiotics; (ii) screening for bioactive compounds; (iii) extraction processes and innovative technologies for purification and chemical characterization; (iv) energy production technologies for the exhausted biomass. We provide a general perspective on the techno-economic feasibility and the environmental footprint of the production process, as well as on the definition of legal constraints for the new products production and commercial use.
Early Hemodynamics after Aortic Valve Replacement
Authors: Aitaliyev Serik , Rumbinaitė Egle , Mėlinytė-Ankudavičė Karolina , Nekrošius Rokas , Keturakis Vytenis , Benetis Rimantas
Published: December 2020

Background and objectives: The aims of this study were to investigate changes in the hemodynamics associated with different types of aortic prostheses and to evaluate patient-prosthesis mismatch (PPM) at rest and after exercise. Materials and Methods: We retrospectively analyzed 150 patients who presented with indications for aortic valve replacement (AVR) with/without concomitant surgery from March 2019 to January 2020. The study population included 90 (60%) men and 60 (40%) women (mean age, 67.33 ± 10.22 years; range, 37–88 years). Echocardiography data such as peak and mean transprosthetic pressure gradients (Gmax, Gmean), velocity (V), effective orifice area (EOA), and indexed EOA (iEOA) were derived at rest and after exercise at baseline and before discharge. The study patients performed the six-minute walk test (6MWT) on the 5th–7th postoperative day. Results: Stented tissue valves showed excellent performance at rest and after exercise in comparison with mechanical valves, which showed favorable hemodynamics at rest only. At the time of discharge, moderate PPM was observed in 7/74 patients (9.5%) at rest and 5/98 (3.3%) patients after exercise. None of the patients showed severe PPM. EOA and iEOA were not significantly different between the groups. However, the stented group showed more pronounced changes in EOA and iEOA after exercise, whereas the changes in the mechanical valve group did not reach significance. Conclusions: In the early postoperative period, mechanical valves and stented valves showed favorable resting hemodynamics. The PPM rate measured after exercise was lower than that at rest.
Functions of Ionic Liquids in Preparing Membranes for Liquid Separations: A Review
Authors: Zheng Dayuan , Hua Dan , Hong Yiping , Ibrahim Abdul-Rauf , Yao Ayan , Pan Junyang , Zhan Guowu
Published: December 2020

Membranes are widely used for liquid separations such as removing solute components from solvents or liquid/liquid separations. Due to negligible vapor pressure, adjustable physical properties, and thermal stability, the application of ionic liquids (ILs) has been extended to fabricating a myriad of membranes for liquid separations. A comprehensive overview of the recent developments in ILs in fabricating membranes for liquid separations is highlighted in this review article. Four major functions of ILs are discussed in detail, including their usage as (i) raw membrane materials, (ii) physical additives, (iii) chemical modifiers, and (iv) solvents. Meanwhile, the applications of IL assisted membranes are discussed, highlighting the issues, challenges, and future perspectives of these IL assisted membranes in liquid separations.
Ball Spin Forming for Flexible and Partial Diameter Reduction in Tubes
Authors: Hirama Shota , Ikeda Takayuki , Gondo Shiori , Kajikawa Shohei , Kuboki Takashi
Published: December 2020

This paper proposed a new ball spin forming equipment, which can form a reduced diameter section on the halfway point of a tube. The effects of forming process parameters on the surface integrity and deformation characteristics of the product were investigated. The proposed method can reduce the diameter in the middle portion of the tube, and the maximum diameter reduction ratio was over 10% in one pass. When the feed pitch of the ball die was more than 2.0 mm/rev, spiral marks remained on the surface of tube. Torsional deformation, axial elongation and an increase in thickness appeared in the tube during the forming process. All of them were affected by the feed pitch and feed direction of the ball die, while they were not affected by the rotation speed of the tube. By sending the ball die towards the fixed part of the tube or by increasing the feed pitch, torsional deformation and elongation decreased, and the amount of thickening increased. When the tube was pressed perpendicularly to the axis without axial feed, a diameter reduction ratio of 21.1% was achieved without defects using a ball diameter of 15.9 mm. The polygonization of the tube was suppressed by reducing the pushing pitch. The ball spin forming has a high advantage in flexible diameter reduction processing on the halfway point of the tube for producing different diameter tubes.
Application of a Low-Cost Cellulose-Based Bioadsorbent for the Effective Recovery of Terbium Ions from Aqueous Solutions
Authors: Alcaraz Lorena , Saquinga Dayana Nathaly , López Floralba , Lima Lola De , Alguacil Francisco J. , Escudero Esther , López Félix A.
Published: December 2020

The preparation of a low-cost cellulose-based bioadsorbent from cellulosic material extracted from rose stems (CRS) was carried out; rose stems are considered agricultural waste. After the required pretreatment of this waste and further treatment with an acidic mixture of acetic and nitric acids, the CRS product was produced. The resulting bioadsorbent was characterized by several techniques, such as X-ray diffraction, which revealed diffraction maxima related to the cellulose structure, whose calculated crystallinity index (CrI) was 75%. In addition, Fourier Transform Infrared spectroscopy (FTIR), 13C Nuclear Magnetic Resonance (NMR), and X-ray Photoelectron Spectroscopy (XPS) showed signs of acetylation of the sample. The thermal properties of the solid were also evaluated through Thermogravimetric Analysis (TGA). Scanning Electron Microscopy (SEM) showed cellulose fibers before and after the adsorption process, and some particles with irregular shapes were also observed. The CRS bioadsorbent was used for the effective adsorption of valuable Tb(III) from an aqueous solution. The adsorption data showed a good fit to the Freundlich isotherm and pseudo-second-order kinetic models; however, chemisorption was not ruled out. Finally, desorption experiments revealed the recovery of terbium ions with an efficiency of 97% from the terbium-loaded bioadsorbent.
The Interaction between Grains during Columnar-to-Equiaxed Transition in Laser Welding: A Phase-Field Study
Authors: Xiong Lingda , Wang Chunming , Wang Zhimin , Jiang Ping
Published: December 2020

A phase-field model was applied to study CET (columnar-to-equiaxed transition) during laser welding of an Al-Cu model alloy. A parametric study was performed to investigate the effects of nucleation undercooling for the equiaxed grains, nucleation density and location of the first nucleation seed ahead of the columnar front on the microstructure of the fusion zone. The numerical results indicated that nucleation undercooling significantly influenced the occurrence and the time of CET. Nucleation density affected the occurrence of CET and the size of equiaxed grains. The dendrite growth behavior was analyzed to reveal the mechanism of the CET. The interactions between different grains were studied. Once the seeds ahead of the columnar dendrites nucleated and grew, the columnar dendrite tip velocity began to fluctuate around a value. It did not decrease until the columnar dendrite got rather close to the equiaxed grains. The undercooling and solute segregation profile evolutions of the columnar dendrite tip with the CET and without the CET had no significant difference before the CET occurred. Mechanical blocking was the major blocking mechanism for the CET. The equiaxed grains formed first were larger than the equiaxed grains formed later due to the decreasing of undercooling. The size of equiaxed grain decreased from fusion line to center line. The numerical results were basically consistent with the experimental results obtained by laser welding of a 2A12 Al-alloy.
Self-Diffusion in Liquid Copper, Silver, and Gold
Authors: Dubinin Nikolay
Published: December 2020

The recently developed by us semi-analytical representation of the mean spherical approximation in conjunction with the linear trajectory approximation is applied to the quantitative study of self-diffusivities in liquid Cu, Ag and Au at different temperatures. The square-well model is employed for the description of the interatomic pair interactions in metals under study. It is found that our theoretical results are in good agreement with available experimental and computer-simulation data and can be considered as a prediction when such data are absent.
Fabrication and Characterization of Single-Crystal Diamond Membranes for Quantum Photonics with Tunable Microcavities
Authors: Heupel Julia , Pallmann Maximilian , Körber Jonathan , Merz Rolf , Kopnarski Michael , Stöhr Rainer , Reithmaier Johann Peter , Hunger David , Popov Cyril
Published: December 2020

The development of quantum technologies is one of the big challenges in modern research. A crucial component for many applications is an efficient, coherent spin–photon interface, and coupling single-color centers in thin diamond membranes to a microcavity is a promising approach. To structure such micrometer thin single-crystal diamond (SCD) membranes with a good quality, it is important to minimize defects originating from polishing or etching procedures. Here, we report on the fabrication of SCD membranes, with various diameters, exhibiting a low surface roughness down to 0.4 nm on a small area scale, by etching through a diamond bulk mask with angled holes. A significant reduction in pits induced by micromasking and polishing damages was accomplished by the application of alternating Ar/Cl2 + O2 dry etching steps. By a variation of etching parameters regarding the Ar/Cl2 step, an enhanced planarization of the surface was obtained, in particular, for surfaces with a higher initial surface roughness of several nanometers. Furthermore, we present the successful bonding of an SCD membrane via van der Waals forces on a cavity mirror and perform finesse measurements which yielded values between 500 and 5000, depending on the position and hence on the membrane thickness. Our results are promising for, e.g., an efficient spin–photon interface.
Long Carbon Fibers for Microwave Absorption: Effect of Fiber Length on Absorption Frequency Band
Authors: Breiss Hanadi , El Assal Aicha , Benzerga Ratiba , Méjean Chloé , Sharaiha Ala
Published: December 2020

This work presents lightweight epoxy foams loaded with very low weight percentages (≤0.5 wt.%) of carbon fibers (CFs) with different lengths (3 mm, 6 mm, and 12 mm) as broadband microwave absorbing materials for anechoic chamber application. The effect of CF length on microwave absorption, especially on the absorption frequency band, is investigated for frequencies between 1 and 15 GHz. For the elaboration of composites, three different methods—spatula, shear mixing, and ultrasounds—are used for the dispersion of CFs. The observation of these CFs, after the dispersion step, shows a high fiber breakage rate when shear mixing is used, unlike when spatula or ultrasounds methods are used. On the other hand, the characterization of the elaborated composites highlights a correlation between the mixing methods, hence the fiber brakeage, and the measured reflection coefficient (reflection loss) of the composites. As a result, the minimum value of the reflection coefficient is shifted toward the high frequencies when the fiber breakage is observed, suggesting that short CFs absorb at high frequencies while long CFs absorb at low frequencies. Dielectric properties, extracted from the measurement in free space, of composites elaborated with different fiber lengths (3 mm, 6 mm, and 12 mm) confirm that short CFs (3 mm) show maximum losses at high frequencies (around 15 GHz) while long CFs (12 mm) show maximum dielectric losses at low frequencies (below 4 GHz). However, no significant variation is observed on the real part of the relative permittivity, as a function of fiber length, for these porous composites loaded with very low CF rates. A hybrid composite, with a mix of different CF lengths, is prepared and characterized. The simulation of the absorption performance of a pyramidal absorber, based on this hybrid composite, is compared to the one of pyramidal absorber based on composites loaded with a single length of carbon fibers. The pyramidal absorber-based hybrid composite predicts the best absorption performance, especially at the low frequency band. The simulated reflection coefficient of this absorber is less than −12 dB in all the studied frequency range, and less than −40 dB for frequencies higher than 3 GHz. This result confirms the interest of using a mix of carbon fiber lengths to achieve a broadband microwave absorber.
Fabrication of a 3D Multi-Depth Reservoir Micromodel in Borosilicate Glass Using Femtosecond Laser Material Processing
Authors: Owusu-Ansah Ebenezer , Dalton Colin
Published: December 2020

Micromodels are ideal candidates for microfluidic transport investigations, and they have been used for many applications, including oil recovery and carbon dioxide storage. Conventional fabrication methods (e.g., photolithography and chemical etching) are beset with many issues, such as multiple wet processing steps and isotropic etching profiles, making them unsuitable to fabricate complex, multi-depth features. Here, we report a simpler approach, femtosecond laser material processing (FLMP), to fabricate a 3D reservoir micromodel featuring 4 different depths—35, 70, 140, and 280 µm, over a large surface area (20 mm × 15 mm) in a borosilicate glass substrate. The dependence of etch depth on major processing parameters of FLMP, i.e., average laser fluence (LFav), and computer numerically controlled (CNC) processing speed (PSCNC), was studied. A linear etch depth dependence on LFav was determined while a three-phase exponential decay dependence was obtained for PSCNC. The accuracy of the method was investigated by using the etch depth dependence on PSCNC relation as a model to predict input parameters required to machine the micromodel. This study shows the capability and robustness of FLMP to machine 3D multi-depth features that will be essential for the development, control, and fabrication of complex microfluidic geometries.
Machine Learning-Based Pipeline for High Accuracy Bioparticle Sizing
Authors: Luo Shaobo , Zhang Yi , Nguyen Kim Truc , Feng Shilun , Shi Yuzhi , Liu Yang , Hutchinson Paul , Chierchia Giovanni , Talbot Hugues , Bourouina Tarik , Jiang Xudong , Liu Ai Qun
Published: December 2020

High accuracy measurement of size is essential in physical and biomedical sciences. Various sizing techniques have been widely used in sorting colloidal materials, analyzing bioparticles and monitoring the qualities of food and atmosphere. Most imaging-free methods such as light scattering measure the averaged size of particles and have difficulties in determining non-spherical particles. Imaging acquisition using camera is capable of observing individual nanoparticles in real time, but the accuracy is compromised by the image defocusing and instrumental calibration. In this work, a machine learning-based pipeline is developed to facilitate a high accuracy imaging-based particle sizing. The pipeline consists of an image segmentation module for cell identification and a machine learning model for accurate pixel-to-size conversion. The results manifest a significantly improved accuracy, showing great potential for a wide range of applications in environmental sensing, biomedical diagnostical, and material characterization.
Eosinopenia in COVID-19 Patients: A Retrospective Analysis
Authors: Ndieugnou Djangang Narcisse , Peluso Lorenzo , Talamonti Marta , Izzi Antonio , Gevenois Pierre Alain , Garufi Alessandra , Goffard Jean-Christophe , Henrard Sophie , Severgnini Paolo , Vincent Jean-Louis , Creteur Jacques , Taccone Fabio Silvio
Published: December 2020

Objectives: The aim of this study was to assess the diagnostic role of eosinophils count in COVID-19 patients. Methods: Retrospective analysis of patients admitted to our hospital with suspicion of COVID-19. Demographic, clinical and laboratory data were collected on admission. Eosinopenia was defined as eosinophils < 100 cells/mm3. The outcomes of this study were the association between eosinophils count on admission and positive real-time reverse transcription polymerase chain reaction (rRT-PCR) test and with suggestive chest computerized tomography (CT) of COVID-19 pneumonia. Results: A total of 174 patients was studied. Of those, 54% had positive rRT-PCR for SARS-CoV-2. A chest CT-scan was performed in 145 patients; 71% showed suggestive findings of COVID-19. Eosinophils on admission had a high predictive accuracy for positive rRT-PCR and suggestive chest CT-scan (area under the receiver operating characteristic—ROC curve, 0.84 (95% CIs 0.78–0.90) and 0.84 (95% CIs 0.77–0.91), respectively). Eosinopenia and high LDH were independent predictors of positive rRT-PCR, whereas eosinopenia, high body mass index and hypertension were predictors for suggestive CT-scan findings. Conclusions: Eosinopenia on admission could predict positive rRT-PCR test or suggestive chest CT-scan for COVID-19. This laboratory finding could help to identify patients at high-risk of COVID-19 in the setting where gold standard diagnostic methods are not available.
The T3SS of Shigella: Expression, Structure, Function, and Role in Vacuole Escape
Authors: Bajunaid Waad , Haidar-Ahmad Nathaline , Kottarampatel Anwer Hasil , Ourida Manigat France , Silué Navoun , F. Tchagang Caetanie , Tomaro Kyle , Campbell-Valois François-Xavier
Published: December 2020

Shigella spp. are one of the leading causes of infectious diarrheal diseases. They are Escherichia coli pathovars that are characterized by the harboring of a large plasmid that encodes most virulence genes, including a type III secretion system (T3SS). The archetypal element of the T3SS is the injectisome, a syringe-like nanomachine composed of approximately 20 proteins, spanning both bacterial membranes and the cell wall, and topped with a needle. Upon contact of the tip of the needle with the plasma membrane, the injectisome secretes its protein substrates into host cells. Some of these substrates act as translocators or effectors whose functions are key to the invasion of the cytosol and the cell-to-cell spread characterizing the lifestyle of Shigella spp. Here, we review the structure, assembly, function, and methods to measure the activity of the injectisome with a focus on Shigella, but complemented with data from other T3SS if required. We also present the regulatory cascade that controls the expression of T3SS genes in Shigella. Finally, we describe the function of translocators and effectors during cell-to-cell spread, particularly during escape from the vacuole, a key element of Shigella’s pathogenesis that has yet to reveal all of its secrets.
T Cell Immunity and the Quest for Protective Vaccines against Staphylococcus aureus Infection
Authors: Armentrout Erin , Liu George , Martins Gislâine
Published: December 2020

Staphylococcus aureus is a wide-spread human pathogen, and one of the top causative agents of nosocomial infections. The prevalence of antibiotic-resistant S. aureus strains, which are associated with higher mortality and morbidity rates than antibiotic-susceptible strains, is increasing around the world. Vaccination would be an effective preventive measure against S. aureus infection, but to date, every vaccine developed has failed in clinical trials, despite inducing robust antibody responses. These results suggest that induction of humoral immunity does not suffice to confer protection against the infection. Evidence from studies in murine models and in patients with immune defects support a role of T cell-mediated immunity in protective responses against S. aureus. Here, we review the current understanding of the mechanisms underlying adaptive immunity to S. aureus infections and discuss these findings in light of the recent S. aureus vaccine trial failures. We make the case for the need to develop anti-S. aureus vaccines that can specifically elicit robust and durable protective memory T cell subsets.
Metabolic Engineering for Unusual Lipid Production in Yarrowia lipolytica
Authors: Park Young-Kyoung , Nicaud Jean-Marc
Published: December 2020

Using microorganisms as lipid-production factories holds promise as an alternative method for generating petroleum-based chemicals. The non-conventional yeast Yarrowia lipolytica is an excellent microbial chassis; for example, it can accumulate high levels of lipids and use a broad range of substrates. Furthermore, it is a species for which an array of efficient genetic engineering tools is available. To date, extensive work has been done to metabolically engineer Y. lipolytica to produce usual and unusual lipids. Unusual lipids are scarce in nature but have several useful applications. As a result, they are increasingly becoming the targets of metabolic engineering. Unusual lipids have distinct structures; they can be generated by engineering endogenous lipid synthesis or by introducing heterologous enzymes to alter the functional groups of fatty acids. In this review, we describe current metabolic engineering strategies for improving lipid production and highlight recent researches on unusual lipid production in Y. lipolytica.
Simultaneous Genome Sequencing of Prosthecochloris ethylica and Desulfuromonas acetoxidans within a Syntrophic Mixture Reveals Unique Pili and Protein Interactions
Authors: Kyndt John A. , Van Beeumen Jozef J. , Meyer Terry E.
Published: December 2020

Strains of Chloropseudomonas ethylica, 2-K, N2, and N3 are known to be composed of a syntrophic mixture of a green sulfur bacterium and a sulfur-reducing colorless component. Upon sequence analysis, the green sulfur photosynthetic bacterial component of strain N3 was dominant and was readily sequenced, but the less abundant sulfur-reducing bacterial component was apparent only when analyzed by metagenomic binning. Whole-genome comparison showed that the green bacterium belonged to the genus Prosthecochloris and apparently was a species for which there was no genome sequence on file. For comparison, we also sequenced the genome of Prosthecochloris sp. DSM 1685, which had previously been isolated from the 2-K mixture in pure culture and have shown that all three Prosthecochloris genomes belong to a new species, which we propose to be named Prosthecochloris ethylica comb. nov. Whole genomes were also sequenced for the isolated Desulfuromonas strains DSM 1675 (from strain 2-K) and DSM 1676 (from strain N2) and shown to be nearly identical to the genome found in the N3 mixture. The genome of the green sulfur bacterium contains large genes for agglutination proteins, similar to the ones proposed to be involved in larger photosynthetic consortia of Chlorochromatium aggregatum. In addition, we also identified several unique “tight adhesion (tad)” pili genes that are presumably involved in the formation of cell–cell interactions. The colorless component, on the other hand, contained a unique large multiheme cytochrome C and unique genes for e-pili (geopilin) formation, genetically clustered with a conserved ferredoxin gene, which are all expected to play an electron transfer role in the closed sulfur cycle in the syntrophic mixture. The findings from the simultaneous genome sequencing of the components of Cp. ethylica have implications for the phenomenon of direct interspecies interactions and coupled electron transfer in photosynthetic symbionts. The mechanisms for such interactions appear to be more common in the environment than originally anticipated.
A Personalized Patient-Centered Intervention to Empower through Physical Activity the Patient in the Dialysis Center: Study Protocol for a Pragmatic Nonrandomized Clinical Trial
Authors: Manfredini Fabio , Lamberti Nicola , Battaglia Yuri , Straudi Sofia , Belvederi Murri Martino , Donadi Maria , Piva Giovanni , Fabbian Fabio , López-Soto Pablo Jesús , Grassi Luigi , Manfredini Roberto , Basaglia Nino , Storari Alda
Published: December 2020

Sedentariness of patients affected by end-stage kidney disease (ESKD) expose them to high risk of unfavorable clinical outcomes. Exercise training is effective in improving physical function, quality of life (QoL) and long-term outcomes. However, the existing barriers related to patients, programs and dialysis staff limit patient participation and call for new strategies. This pragmatic nonrandomized trial will test the impact on ESKD population of an intervention proposed by an exercise facilitator regularly present in a dialysis center. The patient will be free to choose among three-month walking and/or resistance low-intensity training programs: (a) guided physical activity increase; (b) home-based exercise; (c) in-hospital (pre/post dialysis) supervised exercise; (d) performance assessment only. The first phase will define feasibility and the characteristics and preference of responders. The second phase will evaluate safety and patients’ adherence. Outcome measures will be collected at baseline, after three-month and at six-month follow-up. They will include: aerobic capacity, QoL, gait speed, strength, depression and long-term clinical outcomes (hospitalization and mortality). The trial was approved by the Area-Vasta Emilia-Romagna Centro Ethics Committee with approval number 48/2019. Written informed consent will be obtained from all participants. The results of the study will be presented in international congresses, published in peer-reviewed journals and communicated to the patient community. Registration details: Clinicaltrials.gov NCT04282616 [Registered:24/02/2020].
Impact of Quercetin Encapsulation with Added Phytosterols on Bilayer Membrane and Photothermal-Alteration of Novel Mixed Soy Lecithin-Based Liposome
Authors: Toopkanloo Sahar Pakbaten , Tan Tai Boon , Abas Faridah , Alharthi Fahad A. , Nehdi Imededdine Arbi , Tan Chin Ping
Published: December 2020

This study used highly lipophilic agents with an aim to increase the oxidant inhibitory activity and enhance photothermal stability of a novel mixed soy lecithin (ML)-based liposome by changing the composition of formulation within the membrane. Specifically, the development and optimization of the liposome intended for improving Trolox equivalent antioxidant capacity (TEAC) value and %TEAC loss was carried out by incorporating a natural antioxidant, quercetin (QU). In this context, a focus was set on QU encapsulation in ML-based liposomes and the concentration-dependent solubility of QU was investigated and calculated as encapsulation efficiency (EE). To explore the combined effects of the incorporation of plant sterols on the integrity and entrapment capacity of mixed phospholipid vesicles, conjugation of two types of phytosterols (PSs), namely β-sitosterol (βS) and stigmasterol (ST), to mixed membranes at different ratios was also performed. The EE measurement revealed that QU could be efficiently encapsulated in the stable ML-based liposome using 0.15 and 0.1 g/100 mL of βS and ST, respectively. The aforementioned liposome complex exhibited a considerable TEAC (197.23%) and enhanced TEAC loss (30.81%) when exposed to ultraviolet (UV) light (280–320 nm) over a 6 h duration. It appeared that the presence and type of PSs affect the membrane-integration characteristics as well as photodamage transformation of the ML-based liposome. The association of QU with either βS or ST in the formulation was justified by their synergistic effects on the enhancement of the EE of liposomes. Parallel to this, it was demonstrated that synergistic PS effects could be in effect in the maintenance of membrane order of the ML-based liposome. The findings presented in this study provided useful information for the development and production of stable QU-loaded ML-based liposomes for food and nutraceutical applications and could serve as a potential mixed lipids-based delivery system in the disease management using antioxidant therapy.
High-Temperature Atomic Layer Deposition of GaN on 1D Nanostructures
Authors: Austin Aaron J. , Echeverria Elena , Wagle Phadindra , Mainali Punya , Meyers Derek , Gupta Ashish Kumar , Sachan Ritesh , Prassana S. , McIlroy David N.
Published: December 2020

Silica nanosprings (NS) were coated with gallium nitride (GaN) by high-temperature atomic layer deposition. The deposition temperature was 800 °C using trimethylgallium (TMG) as the Ga source and ammonia (NH3) as the reactive nitrogen source. The growth of GaN on silica nanosprings was compared with deposition of GaN thin films to elucidate the growth properties. The effects of buffer layers of aluminum nitride (AlN) and aluminum oxide (Al2O3) on the stoichiometry, chemical bonding, and morphology of GaN thin films were determined with X-ray photoelectron spectroscopy (XPS), high-resolution x-ray diffraction (HRXRD), and atomic force microscopy (AFM). Scanning and transmission electron microscopy of coated silica nanosprings were compared with corresponding data for the GaN thin films. As grown, GaN on NS is conformal and amorphous. Upon introducing buffer layers of Al2O3 or AlN or combinations thereof, GaN is nanocrystalline with an average crystallite size of 11.5 ± 0.5 nm. The electrical properties of the GaN coated NS depends on whether or not a buffer layer is present and the choice of the buffer layer. In addition, the IV curves of GaN coated NS and the thin films (TF) with corresponding buffer layers, or lack thereof, show similar characteristic features, which supports the conclusion that atomic layer deposition (ALD) of GaN thin films with and without buffer layers translates to 1D nanostructures.
Identification of Barriers to Adherence to a Weight Loss Diet in Women Using the Nominal Group Technique
Authors: De Leon Angela , Roemmich James N. , Casperson Shanon L.
Published: December 2020

Background: At any given time, a majority of women are engaged in some type of weight loss diet; however, these efforts are difficult to sustain for long-term weight control. Because women are more likely to develop obesity and suffer a greater severity of obesity-related health and economic consequences, we sought to identify the key factors that make adhering to a weight loss diet difficult for overweight/obese women. Methods: Ten nominal group technique (NGT) sessions aimed at identifying perceived barriers to adherence to a weight loss diet were conducted as part of a weight loss study for overweight/obese women (n = 33) during the controlled feeding weight loss phase. Results: Individual-level barriers to emerge from the sessions included knowing when to stop eating, being able to control cravings and emotional eating, and sustaining healthier dietary habits. Environmental-level barriers included family/social events that bring people together, especially those centered around food and drink, eating out, cost, and busy schedules. Conclusions: These findings offer a deeper understanding of barriers women find most salient to adhering to a weight loss diet, providing direction for the clinical application of weight loss programs.
Chlorella vulgaris Ameliorates Oxidative Stress and Improves the Muscle Regenerative Capacity of Young and Old Sprague-Dawley Rats
Authors: Zainul Azlan Nurhazirah , Mohd Yusof Yasmin Anum , Makpol Suzana
Published: December 2020

Muscle atrophy in ageing is a multifactorial degenerative process impacted by cellular ageing biology, which includes oxidative stress. Chlorella vulgaris is a coccoid green eukaryotic microalga rich in antioxidants. The aim of this study was to determine the effect of C. vulgaris in ameliorating oxidative stress, thus elucidating its mechanism in improving muscle mass, strength and function in young and old rats. Fifty-six male Sprague-Dawley (SD) rats aged 3 months (young) and 21 months (old) were divided into three groups: Group 1 (control) was given distilled water; Group 2 was treated with 150 mg/kg body weight (BW) of C. vulgaris; and Group 3 was treated with 300 mg/kg BW of C. vulgaris for three months. Grip and muscle strength and muscle integrity were determined on days 0, 30, 60, and 90 of treatment. Urine and blood were collected on days 0 and 90 of treatment for oxidative stress marker determination, while the gastrocnemius muscles were collected for muscle oxidative stress analysis. Increased grip strength of the front and hind paws was observed in young C. vulgaris-treated rats on days 30, 60, and 90 compared to the untreated control on the same days (p < 0.05). There was a significant increase in lean bone mineral content (BMC) in young rats treated with 300 mg/kg BW C. vulgaris compared to untreated rats on days 30 and 60. The fat mass was significantly decreased in young and old C. vulgaris-treated rats on day 90 compared to the untreated control. The total path was significantly increased for old rats treated with 300 mg/kg BW C. vulgaris on days 60 and 90 compared to day 0. Young and old C. vulgaris-treated rats demonstrated a significant decrease in urinary isoprostane F2t and plasma creatine kinase-MM (CKMM) compared to the control on day 90. A significant decrease in malondialdehyde (MDA) and 4-hydroxyalkenal (HAE) levels were observed in young and old rats treated with C. vulgaris. C. vulgaris improved the muscle mass, strength, and function in young and old rats. This effect could be due to its potency in ameliorating oxidative stress in the skeletal muscle of young and old rats.
Analysis of the Impact of a Multi-Strain Probiotic on Body Composition and Cardiorespiratory Fitness in Long-Distance Runners
Authors: Smarkusz-Zarzecka Joanna , Ostrowska Lucyna , Leszczyńska Joanna , Orywal Karolina , Cwalina Urszula , Pogodziński Damian
Published: December 2020

Use of probiotic supplements, the benefits of which have not been proven in sportspeople, is becoming more widespread among runners. The aim of this study was to evaluate the effect of a multi-strain probiotic on body composition, cardiorespiratory fitness and inflammation in the body. The randomised, double-blind study included 66 long-distance runners. The intervention factor was a multi-strain probiotic or placebo. At the initial and final stages of the study, evaluation of body composition and cardiorespiratory fitness was performed and the presence of inflammation determined. In the group of men using the probiotic, an increase in lean body mass (p = 0.019) and skeletal muscle mass (p = 0.022) was demonstrated, while in the group of women taking the probiotic, a decrease in the content of total body fat (p = 0.600) and visceral fat (p = 0.247) was observed. Maximum oxygen consumption (VO2max) increased in women (p = 0.140) and men (p = 0.017) using the probiotic. Concentration of tumour necrosis factor-alpha decreased in women (p = 0.003) and men (p = 0.001) using the probiotic and in women (p = 0.074) and men (p = 0.016) using the placebo. Probiotic therapy had a positive effect on selected parameters of body composition and cardiorespiratory fitness of study participants and showed a tendency to reduce inflammation.
The Gut Microbiome, Aging, and Longevity: A Systematic Review
Authors: Badal Varsha D. , Vaccariello Eleonora D. , Murray Emily R. , Yu Kasey E. , Knight Rob , Jeste Dilip V. , Nguyen Tanya T.
Published: December 2020

Aging is determined by complex interactions among genetic and environmental factors. Increasing evidence suggests that the gut microbiome lies at the core of many age-associated changes, including immune system dysregulation and susceptibility to diseases. The gut microbiota undergoes extensive changes across the lifespan, and age-related processes may influence the gut microbiota and its related metabolic alterations. The aim of this systematic review was to summarize the current literature on aging-associated alterations in diversity, composition, and functional features of the gut microbiota. We identified 27 empirical human studies of normal and successful aging suitable for inclusion. Alpha diversity of microbial taxa, functional pathways, and metabolites was higher in older adults, particularly among the oldest-old adults, compared to younger individuals. Beta diversity distances significantly differed across various developmental stages and were different even between oldest-old and younger-old adults. Differences in taxonomic composition and functional potential varied across studies, but Akkermansia was most consistently reported to be relatively more abundant with aging, whereas Faecalibacterium, Bacteroidaceae, and Lachnospiraceae were relatively reduced. Older adults have reduced pathways related to carbohydrate metabolism and amino acid synthesis; however, oldest-old adults exhibited functional differences that distinguished their microbiota from that of young-old adults, such as greater potential for short-chain fatty acid production and increased butyrate derivatives. Although a definitive interpretation is limited by the cross-sectional design of published reports, we integrated findings of microbial composition and downstream functional pathways and metabolites, offering possible explanations regarding age-related processes.