Green synthesis of selenium nanoparticles using clove and lemon grass and its antibacterial activity against E. faecalis
Authors: Padmalochini S , Rajeshkumar S , Lakshmi T , Tharani M 
Published: February 2023

Introduction: Nanotechnology is the branch of science and engineering which involves designing and using devices and systems by
manipulating atoms and molecules at nanoscale. Nanotechnology has a wide application in the field of medicine and dentistry which
resulted in the emergence of a new field known as Nanomedicine. In this study Clove and lemongrass are preferred for Green synthesis
of Selenium because to check the anti- bacterial activity of the clove and lemongrass extracted selenium nanoparticle against
enterococcus faecalis. Selenium has anti- cancer and anti- microbial properties, Gold which has DNA labeling, biosensor, drug delivery,
cancer therapy and anti- microbial properties.
Aim:The aim of this study is to evaluate the antibacterial activity of selenium nanoparticles prepared using clove and lemongrass against
e faecalis.
Materials and methods: Antibacterial activity of Selenium nanoparticles acts against the strain e faecalis. Mueller Hinton Agar was
utilized for this activity to determine the zone of inhibition. Mueller hinton Agar were prepared and sterilized for 15 minutes at 121℃.
Media poured into the sterilized plates and let it stable for solidification. The wells were cut using a 9mm sterile polystyrene tip and the
test organisms were swabbed. The selenium nanoparticles with different concentrations (25μL, 50μL, 100μL) were loaded and in the
fourth well standard antibiotic amoxyrite was loaded. The plates were incubated for 24 hours at 37℃. After the incubation time the zone
of inhibition was measured.
Result: The results of the minimum inhibitory concentration values of the selenium nanoparticles is shown in table 1. In 100 μL
concentration the MIC value decreased. The minimum bactericidal concentration of the selenium nanoparticles is shown in figure 4. In
100 μL concentration there is an increased MBC effect seen. The MIC of e faecalis is shown in Graph 1. The X axis represents the different
concentrations of selenium nanoparticles (25μL, 50μL, 100μL) and standard antibiotic amoxyrite. The Y axis represents the absorbents.
As the concentration of the nanoparticle increased the MIC also increased.
Discussion: In the present study MIC and MBC values have increased with respect to 100 μL concentration of the selenium nanoparticles.
In a study conducted by Rangrazi et al have concluded with the same results that the minimum inhibitory concentration (MIC) and
minimum bactericidal concentration (MBC) of the selenium nanoparticles against gram-negative and gram-positive bacteria showed MIC
values of 0.068, 0.137, and 0.274 mg ml−1. The results proved that the concentration of 0.274 mg ml−1 had a higher bactericidal effect
than the other concentrations. As the concentration of selenium nanoparticles increased to 0.274 mg ml−1 the bacteria were completely
killed.
Conclusion: Within the limits of the study it can be concluded that the green synthesized selenium nanoparticles using clove and
lemongrass have remarkable antibacterial potentiality and can be added to dental materials to enhance their properties. Further animal
studies are required for the evaluation of the efficacy of the nanoparticles.