bluuebunny/crossref_metadata_2025 huggingface dataset:
Unraveling the role of disulfidptosis-related LncRNAs in colon cancer: a prognostic indicator for immunotherapy response, chemotherapy sensitivity, and insights into cell death mechanisms
Authors: Chi Hao , Huang Jinbang , Yan Yang , Jiang Chenglu , Zhang Shengke , Chen Haiqing , Jiang Lai , Zhang Jieying , Zhang Qinghong , Yang Guanhu , Tian Gang
Published: October 2023

Background: Colon cancer, a prevalent and deadly malignancy worldwide, ranks as the third leading cause of cancer-related mortality. Disulfidptosis stress triggers a unique form of programmed cell death known as disulfidoptosis, characterized by excessive intracellular cystine accumulation. This study aimed to establish reliable bioindicators based on long non-coding RNAs (LncRNAs) associated with disulfidptosis-induced cell death, providing novel insights into immunotherapeutic response and prognostic assessment in patients with colon adenocarcinoma (COAD).

Methods: Univariate Cox proportional hazard analysis and Lasso regression analysis were performed to identify differentially expressed genes strongly associated with prognosis. Subsequently, a multifactorial model for prognostic risk assessment was developed using multiple Cox proportional hazard regression. Furthermore, we conducted comprehensive evaluations of the characteristics of disulfidptosis response-related LncRNAs, considering clinicopathological features, tumor microenvironment, and chemotherapy sensitivity. The expression levels of prognosis-related genes in COAD patients were validated using quantitative real-time fluorescence PCR (qRT-PCR). Additionally, the role of ZEB1-SA1 in colon cancer was investigated through CCK8 assays, wound healing experiment and transwell experiments.

Results: disulfidptosis response-related LncRNAs were identified as robust predictors of COAD prognosis. Multifactorial analysis revealed that the risk score derived from these LncRNAs served as an independent prognostic factor for COAD. Patients in the low-risk group exhibited superior overall survival (OS) compared to those in the high-risk group. Accordingly, our developed Nomogram prediction model, integrating clinical characteristics and risk scores, demonstrated excellent prognostic efficacy. In vitro experiments demonstrated that ZEB1-SA1 promoted the proliferation and migration of COAD cells.

Conclusion: Leveraging medical big data and artificial intelligence, we constructed a prediction model for disulfidptosis response-related LncRNAs based on the TCGA-COAD cohort, enabling accurate prognostic prediction in colon cancer patients. The implementation of this model in clinical practice can facilitate precise classification of COAD patients, identification of specific subgroups more likely to respond favorably to immunotherapy and chemotherapy, and inform the development of personalized treatment strategies for COAD patients based on scientific evidence.
Characteristics and antioxidant activity of Maillard reaction products from β-lactoglobulin and isomaltooligosaccharide
Authors: Wang Qingyu , Li Jiayang , Tu Yaqi , Cai Jianping , Ren Fazheng , Zhang Hao
Published: October 2023

Starch-derived isomaltooligosaccharide (IMO) is potentially used as prebiotics in infant formulas. Given that they are non-digestible carbohydrates rich in reducing substrates, it’s crucial to understand if they can interact with β-lactoglobulin (β-LG) to produce Maillard reaction products (MRPs) and how these MRPs might influence the nutritional properties of β-LG. In our investigation, we conjugated β-LG with IMO to generate MRPs. Using a spectrophotometer, we identified the intermediates and assessed browning. We also evaluated changes in free amino groups and structural alterations. The antioxidative activity of the resulting compounds was assessed using DPPH and the ferric reducing/antioxidant power (FRAP) assay. Our data revealed increased visible absorption and fluorescence intensity, suggesting the formation of intermediate and browning products. The content of free amino groups diminished by 33%, supporting the conjugation of IMO with β-LG. However, circular dichroism results indicated no significant alterations in the secondary structure of β-LG. Notably, the β-LG-IMO MRPs exhibited enhanced 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and ferric reducing/antioxidant power (FRAP). The findings provide insights into the characteristics and antioxidant activities of the conjugates derived from IMO and dairy protein in infant formula.
Imaging-based adipose biomarkers for predicting clinical outcomes of cancer patients treated with immune checkpoint inhibitors: a systematic review
Authors: Pei Xinyu , Xie Ye , Liu Yixuan , Cai Xinyang , Hong Lexuan , Yang Xiaofeng , Zhang Luyao , Zhang Manhuai , Zheng Xinyi , Ning Kang , Fang Mengyuan , Tang Huancheng
Published: October 2023

Background

Since the application of Immune checkpoint inhibitors (ICI), the clinical outcome for metastatic cancer has been greatly improved. Nevertheless, treatment response varies in patients, making it urgent to identify patients who will receive clinical benefits after ICI therapy. Adipose body composition has proved to be associated with tumor response. In this systematic review, we aimed to summarize the current evidence on imaging adipose biomarkers that predict clinical outcomes in patients treated with ICI in various cancer types.

Methods

Embase and PubMed were searched from database inception to 1st February 2023. Articles included investigated the association between imaging-based adipose biomarkers and the clinical outcomes of patients treated with ICI. The methodological quality of included studies was evaluated through Newcastle- Ottawa Quality Assessment Scale and Radiomics Quality Score tools.

Results

Totally, 22 studies including 2256 patients were selected. Non-small cell lung cancer (NSCLC) had the most articles (6 studies), followed by melanoma (5 studies), renal cell carcinoma (RCC) (3 studies), urothelial carcinoma (UC) (2 studies), head and neck squamous cell carcinoma (HNSCC) (1 study), gastric cancer (1 study) and liver cancer (1 study). The remaining 3 studies investigated metastatic solid tumors including various types of cancers. Adipose biomarkers can be summarized into 5 categories, including total fat, visceral fat, subcutaneous fat, intramuscular fat and others, which exerted diverse correlations with patients’ prognosis after being treated with ICI in different cancers. Most biomarkers of body fat were positively associated with survival benefits. Nevertheless, more total fat was predictable of worse outcomes in NSCLC, while inter-muscular fat was associated with poor clinical benefits in UC.

Conclusion

There is relatively well-supported evidence for imaging-based adipose biomarkers to predict the clinical outcome of ICI. In general, most of the studies show that adipose tissue is positively correlated with clinical outcomes. This review summarizes the significant biomarkers proven by researches for each cancer type. Further validation and large independent prospective cohorts are needed in the future. The protocol of this systematic review has been registered at the International Prospective Register of Systematic Reviews (http://www.crd.york.ac.uk/PROSPERO, registration no: CRD42023401986).
A clinical-radiomics nomogram based on multimodal ultrasound for predicting the malignancy risk in solid hypoechoic breast lesions
Authors: Shiyan Guo , Liqing Jiang , Yueqiong Yan , Yan Zhang
Published: October 2023

Background

In routine clinical examinations, solid hypoechoic breast lesions are frequently encountered, but accurately distinguishing them poses a challenge. This study proposed a clinical-radiomics nomogram based on multimodal ultrasound that enhances the diagnostic accuracy for solid hypoechoic breast lesions.

Method

This retrospective study analyzed ultrasound strain elastography (SE) and automated breast volume scanner images (ABVS) of 423 solid hypoechoic breast lesions from 423 female patients in our hospital between August 2019 and May 2022. They were assigned to the training (n=296) and validation (n=127) groups in a 7:3 ratio by generating random numbers. Radiomics features were extracted and screened from ABVS and SE images, followed by the calculation of the radiomics score (Radscore) based on these features. Subsequently, a nomogram was constructed through multivariate logistic regression to assess the malignancy risk in breast lesions by combining Radscore with Breast Imaging Reporting and Data System (BI-RADS) scores and clinical risk factors associated with breast malignant lesions. The diagnostic performance, calibration performance, and clinical usefulness of the nomogram were assessed by the area under the curve (AUC) of the receiver operating characteristic curve, the calibration curve, and the decision analysis curve, respectively.

Results

The diagnostic performance of the nomogram is significantly superior to that of both the clinical diagnostic model (BI-RADS model) and the multimodal radiomics model (SE+ABVS radiomics model) in training (AUC: 0.972 vs 0.930 vs 0.941) and validation group (AUC:0.964 vs 0.916 vs 0.933). In addition, the nomogram also exhibited a favorable goodness-of-fit and could lead to greater net benefits for patients.

Conclusion

The nomogram enables a more effective assessment of the malignancy risk of solid hypoechoic breast lesions; therefore, it can serve as a new and efficient diagnostic tool for clinical diagnosis.
The effect of compression and combined compression-tactile stimulation on lower limb somatosensory acuity
Authors: Marchant Ashleigh , Wallwork Sarah B. , Ball Nick , Witchalls Jeremy , Waddington Gordon
Published: October 2023

Background

Lower limb somatosensation and proprioception are important for maintaining balance. Research has shown that compression garments or exposure to textured surfaces, can enhance somatosensation however, little is known about the effect of combined compression and texture on somatosensory acuity in the lower limb. This study aimed to assess the effects of combined compression socks with a plantar textured sole, on lower limb somatosensory acuity.

Methods

Thirty participants completed a somatosensory acuity task (active movement extent discrimination apparatus; AMEDA) under three conditions: barefoot (control condition), standard knee-high compression sock (compression sock), and knee-high compression sock with internal rubber nodules situated on the sole (textured-compression sock). Somatosensory acuity was assessed between the different sock conditions for the (i) entire group, (ii) high performers, and (iii) low performers. It was hypothesized that low performers would see gains wearing either sock, but the greatest improvement would be in the textured-compression sock condition.

Results

AMEDA scores were not significantly different between conditions when the entire group was analyzed (p = 0.078). The low performers showed an improvement in somatosensory acuity when wearing the compression sock (p = 0.037) and the textured compression sock (p = 0.024), when compared to barefoot, but there was no difference between the two sock conditions (p > 0.05). The high performers did not show any improvement (p > 0.05 for all).

Conclusion

These findings demonstrate that additional sensory feedback may be beneficial to individuals with lower baseline somatosensory acuity but is unlikely to provide benefit for those with higher somatosensory acuity.
Preliminary study of neurofilament light chain as a biomarker for hypoxia-induced neuronal injury in dogs
Authors: Yun Taesik , Chae Yeon , Koo Yoonhoi , Lee Dohee , Kim Hakhyun , Yang Mhan-Pyo , Kang Byeong-Teck
Published: October 2023

Neurofilament light chain (NfL) is a neuroaxonal protein in the nervous system. NfL has recently been demonstrated to be a biomarker for various neurological diseases. In this study, we investigated the potential role of NfL in hypoxia-induced neuronal injury in dogs. Serum NfL levels were determined using a single-molecule array. Serum NfL concentrations were significantly higher in hypoxemic dogs without neurological signs (n = 6, 175.5 pg/mL) than in healthy dogs (n = 15, 15.9 pg/mL; p < 0.0001). Therefore, neuronal injury should be considered in dogs with hypoxemia caused by cardiopulmonary diseases, even in the absence of neurological signs.
Earth-Venus Mission Analysis via Weak Capture and Nonlinear Orbit Control
Authors: De Angelis Giulio , Carletta Stefano , Pontani Mauro , Teofilatto Paolo
Published: October 2023

Exploration of Venus is recently driven by the interest of the scientific community in understanding the evolution of Earth-size planets, and is leading the implementation of missions that can benefit from new design techniques and technology. In this work, we investigate the possibility to implement a microsatellite exploration mission to Venus, taking advantage of (i) weak capture, and (ii) nonlinear orbit control. This research considers the case of a microsatellite, equipped with a high-thrust and a low-thrust propulsion system, and placed in a highly elliptical Earth orbit, not specifically designed for the Earth-Venus mission of interest. In particular, to minimize the propellant mass, phase (i) of the mission was designed to inject the microsatellite into a low-energy capture around Venus, at the end of the interplanetary arc. The low-energy capture is designed in the dynamical framework of the circular restricted 3-body problem associated with the Sun-Venus system. Modeling the problem with the use of the Hamiltonian formalism, capture trajectories can be characterized based on their state while transiting in the equilibrium region about the collinear libration point L1. Low-energy capture orbits are identified that require the minimum velocity change to be established. These results are obtained using the General Mission Analysis Tool, which implements planetary ephemeris. After completing the ballistic capture, phase (ii) of the mission starts, and it is aimed at driving the microsatellite toward the operational orbit about Venus. The transfer maneuver is based on the use of low-thrust propulsion and nonlinear orbit control. Convergence toward the desired operational orbit is investigated and is proven analytically using the Lyapunov stability theory, in conjunction with the LaSalle invariance principle, under certain conditions related to the orbit perturbing accelerations and the low-thrust magnitude. The numerical results prove that the mission profile at hand, combining low-energy capture and low-thrust nonlinear orbit control, represents a viable and effective strategy for microsatellite missions to Venus.
Exposure to Cattle Slurry of Different Concentrations Influence Germination and Initial Growth of Selected Grass and Legume Species
Authors: Gselman Anastazija , Sem Vilma , Grobelnik Mlakar Silva
Published: October 2023

In addition to improving soil quality, the fertilisation of grassland with cattle slurry is often associated with seed dispersal. Most studies focus on the effects of cattle slurry on the germination and early development of weed species, but less is known about how slurry affects the germination process of grasses and forage legumes. The aim of Experiment I of our study was therefore to investigate the influence of soaking time in cattle slurry of different concentrations on Lolium multiflorum, Dactylis glomerata, Trifolium pratense and Trifolium repens. Seeds were soaked in undiluted (100%) and diluted cattle slurries (50% and 25%) for 14, 28, 42, 56, 70 and 84 days. Experiment II was conducted to study the initial growth of studied plants from seeds soaked in cattle slurry of different concentrations for 14 days. After the germination test, which was carried out under controlled conditions, the germination index (GI) was calculated. The results (Experiment I) show that a short soaking in cattle slurry (14 days) has no negative effect on the germination process for all species. However, a longer soaking resulted in significantly reduced and delayed germination, especially in undiluted slurry for grasses and diluted slurries for clovers. The slurry concentration (Experiment II) only influenced the root growth of L. multiflorum. Seedlings grown from seeds soaked in undiluted slurry had a 17% higher relative root length than the control and developed significantly longer root systems than the other two slurry concentrations.
Development of Seeding Rate Monitoring System Applicable to a Mechanical Pot-Seeding Machine
Authors: Kim Seung-Jun , Lee Hyeon-Seung , Hwang Seok-Joon , Kim Jeong-Hun , Jang Moon-Kyeong , Nam Ju-Seok
Published: October 2023

In this study, we developed a monitoring system to accurately track the seeding rate and to identify the locations where the mechanical pot-seeding machine failed to sow seeds correctly. The monitoring system employs diverse image processing techniques, including the Hough transform, hue–saturation–value color space conversion, image morphology techniques, and Gaussian blur, to accurately pinpoint the seeding rate and the locations where seeds are missing. To determine the optimal operating conditions for the seeding rate monitoring system, a factorial experiment was conducted by varying the brightness and saturation values of the image data. When the derived optimal operating conditions were applied, the system consistently achieved a 100% seed recognition rate across various seeding conditions. The monitoring system developed in this study has the potential to significantly reduce the labor required for supplementary planting by enabling the real-time identification of locations where seeds were not sown during pot-seeding operations.
Path Planning and Control System Design of an Unmanned Weeding Robot
Authors: Yang Tengxiang , Jin Chengqian , Ni Youliang , Liu Zhen , Chen Man
Published: October 2023

Aiming at the demand by unmanned farms for unmanned operation in the entire process of field management, an unmanned plant protection robot for field management was developed based on a platform comprising a traditional high-clearance spray rod sprayer, integrated unmanned driving technology, image recognition technology, intelligent control technology, and precision operation technology. According to the agricultural machinery operation mode, agricultural machinery path planning, linear path tracking, and header path tracking algorithms were developed. Based on the overall structure and working principle of the chassis, the robot control system, steering control system, and operation control system were set. Based on the YOLOv5 image recognition algorithm, the crop–weed recognition model was developed. After 6000 rounds of training, the accuracy, recall, and mean average precision of the model were 87.7%, 84.5%, and 79.3%, respectively. Finally, a field experiment was carried out with the unmanned plant protection robot equipped with a complete system. Results show that the average lateral error of the robot is 0.036 m, the maximum lateral error is 0.2 m, the average root mean square error is 0.053 m, the average velocity error is 0.034 m/s, and the average root mean square error of velocity is 0.045 m/s when the robot works in a straight line. In weeding operations, the area ratio of weedy zones to field is 25%, which saves 75% of the herbicide compared to that dispensed in full spraying mode. The unmanned plant protection robot designed in this study effectively achieves machinery’s autonomous operation, providing valuable insights for research in unmanned farming and autonomous agricultural machinery.
Agricultural Tractor Retail and Wholesale Residual Value Forecasting Model in Western Europe
Authors: Herranz-Matey Ivan , Ruiz-Garcia Luis
Published: October 2023

The residual value of agricultural tractors plays a pivotal role in the financial viability of agribusiness enterprises. Nevertheless, there is a dearth of comprehensive studies concerning the prognostication of both retail and wholesale residual values specific to agricultural tractors within the context of Western Europe. This research introduces an innovative methodology for assessing the residual worth of agricultural tractors, with particular consideration given to the substantial pricing discrepancies between retail and wholesale transactions. Leveraging publicly available auction data, we develop a polynomial regression model aimed at forecasting the intricate relationship between retail and wholesale residual values. Notably, the model demonstrates an exceptional robustness, surpassing previous research endeavors, as evidenced by a remarkably low root-mean-square error (RMSE) of 0.0159 and a combined adjusted coefficient of determination (RSqAdj) of 0.9997. The findings of this study offer invaluable insights into a diverse array of stakeholders, empowering them to make well-informed decisions regarding machinery specifications, investment strategies, and asset disposal choices, thereby facilitating optimal financial performance.
Performance Evaluation of a Virtual Test Model of the Frame-Type ROPS for Agricultural Tractors Using FEA
Authors: Lim Ryu-Gap , Kim Wan-Soo , Do Young-Woo , Siddique Md. Abu Ayub , Kim Yong-Joo
Published: October 2023

In this study, a model of the frame-type ROPS (rollover protective structure) for an agricultural tractor was developed using FEA (finite-element analysis). Various boundary conditions were applied as input variables to replace the actual test of the ROPS with a virtual test. An optimization study was carried out based on the boundary conditions of the bolt, considering the ROPS part directly mounted on the tractor and the folding connection to the ROPS. The results of the virtual test were compared with those of the actual test, and the error was determined. The maximum error of the evaluation model was 7.0% for the force applied on the load and 9.6% for the corresponding ROPS deformation. All mounting bolts of the ROPS required modeling. In particular, we had to establish free boundary conditions for axial rotation to implement the folding connection. In addition, a simulation of the frame-type ROPS was conducted according to the mesh size. A convenient simulation time was obtained for a mesh size of 8~10 mm. Compared with the actual test, the accuracy was the highest with a mesh size of 6~8 mm.
Analysis of Linkage on Interaction of Main Aspects (Genotype by Environment Interaction, Stability and Genetic Parameters) of 1000 Kernels in Maize (Zea mays L.)
Authors: Nowosad Kamila , Bocianowski Jan , Kianersi Farzad , Pour-Aboughadareh Alireza
Published: October 2023

The assessment of 1000-kernel weight holds significant importance in determining maize grain yield, and elucidating its underlying genetic mechanisms is imperative for enhancing its overall performance. The material for the study consisted of 26 doubled-haploid (DH) maize lines obtained from crossing two cultivars with flint kernels. Lines were planted in the northern part of the Lower Silesia voivodship in Poland over ten years (2013–2022). The 1000-kernel weight was assessed. The purposes of the research were as follows: (1) to assess genotype by environment interaction (GEI by the additive main effects and multiplicative interaction (AMMI) model; (2) the selection of stable DH lines and environment-specific lines; and (3) the estimation of parameters related to additive and additive–additive gene interaction (epistasis). The results indicate the significant effects of genotype and environment, as well as the GEI, on the 1000-kernel weight. Estimates of additive gene action effects were statistically significant in every year of the study, except 2022. Estimates of epistasis (total additive-by-additive interaction) effects for 1000-kernel weight were statistically significant in 2013, 2015, and 2017 (positive effects), as well as in 2018 and 2020 (negative effects). The lines KN07 and KN10 are recommended for further inclusion in the breeding program due to their stability and highest average of 1000-kernel weight.
Feasibility of Photovoltaic Systems for the Agrifood Industry in the New Energy and Climate Change Context
Authors: García José L. , Perdigones Alicia , Benavente Rosa M. , Álvarez José , Baptista Fátima , Mazarrón Fernando R.
Published: October 2023

The role of the agrifood industry is key to mitigating the impact of climate change, as it is one of the industrial sectors with the highest energy consumption. The optimisation of photovoltaic systems in agroindustries faces problems such as the fluctuation of energy prices or the evident seasonal nature of some producers. This paper provides a global view of the profitability and optimal sizing of photovoltaic (PV) systems in the new energy context. For this purpose, almost 4 million cases were analysed, including different consumption patterns, energy prices, etc. Some general conclusions can be drawn from the results. First, the adaptation to the new context requires adjustments in the sizing of PV systems in all industries analysed, which is also associated with changes in the return on investment. Second, seasonality strongly conditions the optimal size of PV installations, the return on the investment and the potential savings. Finally, in the face of future energy price variations, the ratio “Savings/payback” seems to be an appropriate reference for sizing, combining savings and profitability. In addition, they may justify special subsidies to seasonal industries. The conclusions of this paper should be considered to optimise the design of PVs.
The Hoof Color of Australian White Sheep Is Associated with Genetic Variation of the MITF Gene
Authors: Su Peng , Wu Hui , Huang Yangming , Lu Xiaofang , Yin Jing , Zhang Qingfeng , Lan Xianyong
Published: October 2023

Studying the characteristics of mammalian hoof colors is important for genetic improvements in animals. A deeper black hoof color is the standard for breeding purebred Australian White (AUW) sheep and this phenotype could be used as a phenotypic marker of purebred animals. We conducted a genome-wide association study (GWAS) analysis using restriction site associated DNA sequencing (RAD-seq) data from 577 Australian White sheep (black hoof color = 283, grey hoof color = 106, amber hoof color = 186) and performed association analysis utilizing the mixed linear model in EMMAX. The results of GWAS demonstrated that a specific single-nucleotide polymorphism (SNP; g. 33097911G>A) in intron 14 of the microphthalmia-associated transcription factor (MITF) gene was significantly associated with the hoof color in AUW sheep (p = 9.40 × 10−36). The MITF gene plays a key role in the development, differentiation, and functional regulation of melanocytes. Furthermore, the association between this locus and hoof color was validated in a cohort of 212 individuals (black hoof color = 122, grey hoof color = 38, amber hoof color = 52). The results indicated that the hoof color of AUW sheep with GG, AG, and AA genotypes tended to be black, grey, and amber, respectively. This study provided novel insights into hoof color genetics in AUW sheep, enhancing our comprehension of the genetic mechanisms underlying the diverse range of hoof colors. Our results agree with previous studies and provide molecular markers for marker-assisted selection for hoof color in sheep.
Melatonin Protects Bovine Spermatozoa by Reinforcing Their Antioxidant Defenses
Authors: Lavrentiadou Sophia N. , Sapanidou Vasiliki , Tzekaki Elena E. , Margaritis Ioannis , Tsantarliotou Maria P.
Published: October 2023

Cryopreserved semen is widely used in assisted reproductive techniques. Post-thawing spermatozoa endure oxidative stress due to the high levels of reactive oxygen and nitrogen species, which are produced during the freezing/thawing process, and the depletion of antioxidants. To counteract this depletion, supplementation of sperm preparation medium with antioxidants has been widely applied. Melatonin is a hormone with diverse biological roles and a potent antioxidant, with an ameliorative effect on spermatozoa. In the present study, we assessed the effect of melatonin on thawed bovine spermatozoa during their handling. Cryopreserved bovine spermatozoa were thawed and incubated for 60 min in the presence or absence of 100 μΜ melatonin. Also, the effect of melatonin was assessed on spermatozoa further challenged by the addition of 100 μΜ hydrogen peroxide. Spermatozoa were evaluated in terms of kinematic parameters (CASA), viability (trypan blue staining) and antioxidant capacity (glutathione and NBT assay, determination of iNOS levels by Western blot analysis). In the presence of melatonin, spermatozoa presented better kinematic parameters, as the percentage of motile and rapid spermatozoa was higher in the melatonin group. They also presented higher viability and antioxidant status, as determined by the increased cellular glutathione levels and the decreased iNOS protein levels.
Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus Luteum
Authors: Thaqi Granit , Berisha Bajram , Pfaffl Michael W.
Published: October 2023

The study aimed to evaluate the mRNA expression levels of various local novel adipokines, including vaspin, adiponectin, visfatin, and resistin, along with their associated receptors, heat shock 70 protein 5, adiponectin receptor 1, and adiponectin receptor 2, in the corpus luteum (CL) during luteal regression, also known as luteolysis, in dairy cows. We selected Fleckvieh cows in the mid-luteal phase (days 8–12, control group) and administered cloprostenol (PGF analog) to experimentally induce luteolysis. We collected CL samples at different time points following PGF application: before treatment (days 8–12, control group) and at 0.5, 2, 4, 12, 24, 48, and 64 h post-treatment (n = 5) per group. The mRNA expression was measured via real-time reverse transcription polymerase chain reaction (RT-qPCR). Vaspin was characterized by high mRNA levels at the beginning of the regression stage, followed by a significant decrease 48 h and 64 h after PGF treatment. Adiponectin mRNA levels were elevated 48 h after PGF. Resistin showed upregulation 4 h post PGF application. In summary, the alterations observed in the adipokine family within experimentally induced regressing CL tissue potentially play an integral role in the local regulatory processes governing the sequence of events culminating in functional luteolysis and subsequent structural changes in the bovine ovary.
The Yeast and Hypha Phases of Candida krusei Induce the Apoptosis of Bovine Mammary Epithelial Cells via Distinct Signaling Pathways
Authors: Miao Yuhang , Ding Tao , Liu Yang , Zhou Xuezhang , Du Jun
Published: October 2023

Infection with Candida spp. is a significant cause of bovine mastitis globally. We previously found that C. krusei was the main pathogen causing mycotic mastitis in dairy cows in Yinchuan, Ningxia, China. However, whether the infection of this pathogen could induce apoptosis in BMECs remained unclear. In this report, we explored the apoptosis and underlying mechanism of BMECs induced by C. krusei yeast and hypha phases using a pathogen/host cell co-culture model. Our results revealed that both the yeast and hypha phases of C. krusei could induce BMEC apoptosis; however, the yeast phase induced more cell apoptosis than the hypha phase, as assessed via electronic microscopy and flow cytometry assays. This finding was further corroborated via the measurement of the mitochondrial membrane potential (MMP) and the TUNEL test. Infection by both the yeast and hypha phases of C. krusei greatly induced the expression of proteins associated with cell death pathways and important components of toll-like receptor (TLR) signaling, including TLR2 and TLR4 receptors, as determined via a Western blotting assay. BMECs mainly underwent apoptosis after infection by the C. krusei yeast phase through a mitochondrial pathway. Meanwhile, BMEC apoptosis induced by the C. krusei hypha phase was regulated by a death ligand/receptor pathway. In addition, C. krusei-induced BMEC apoptosis was regulated by both the TLR2/ERK and JNK/ERK signaling pathways. These data suggest that the yeast phase and hypha phase of C. krusei induce BMEC apoptosis through distinct cell signaling pathways. This study represents a unique perspective on the molecular processes underlying BMEC apoptosis in response to C. krusei infection.
Optimizing the Adipogenic Induction Protocol Using Rosiglitazone Improves the Physiological Parameters and Differentiation Capacity of Adipose Tissue-Derived Mesenchymal Stem Cells for Horses, Sheep, Dogs, Murines, and Humans
Authors: Heimann Manuela , Elashry Mohamed I. , Klymiuk Michele C. , Eldaey Asmaa , Wenisch Sabine , Arnhold Stefan
Published: October 2023

The investigation of adipose tissue-derived mesenchymal stem cells (ASCs) has received considerable interest in regenerative medicine. A nontoxic adipogenic induction protocol valid for cells of different mammalian species has not been described. This study aims to establish an adipogenic differentiation protocol suitable for horses, sheep, dogs, murines, and human cells. An optimized rosiglitazone protocol, consisting of 5% fetal calf serum in Dulbecco’s Modified Eagle’s Medium, 10 μg/mL insulin, 0.55 μg/mL transferrin, 6.8 ng sodium selenite, 1 μM dexamethasone, and 1–5 μM of rosiglitazone, is compared to the 3-isobutyl-1-methylxantine (IBMX) protocol, where rosiglitazone was replaced with 0.5 mM IBMX and 0.2 mM indomethacin. Cell viability, cytotoxicity, a morphometric analysis of the lipid, and the expression of adipogenic markers for 14 days were assessed. The data revealed that using 5 µM of rosiglitazone promotes the adipogenic differentiation capacity in horse, sheep, and dog cells compared to IBMX induction. Meanwhile, marked reductions in the cell viability and cell number with the IBMX protocol were detected, and rosiglitazone increased the cell number and lipid droplet size, prevented apoptosis, and upregulated FABP-4 and Leptin expression in the cells of most of the species. Our data revealed that the rosiglitazone protocol improves the adipogenesis of ASCs, together with having less toxicity, and should be considered for cell reproducibility and clinical applications targeting obesity.
Effects of Olea europaea L. Polyphenols on the Animal Welfare and Milk Quality in Dairy Cows
Authors: Di Meo Maria Chiara , Giacco Antonia , Zarrelli Armando , Mandrone Vittorio Maria , D’Angelo Livia , Silvestri Elena , De Girolamo Paolo , Varricchio Ettore
Published: October 2023

Here, we evaluated the effect of dietary supplementation with an Olea europaea L. extract on the animal welfare and milk quality of dairy cows. Thirty Italian Holstein–Friesian dairy cows in the mid-lactation phase (90 to 210 days) were blocked into experimental groups based on parity class (namely, primiparous (P) (n = 10), secondiparous (S) (n = 10) and pluriparous (PL) (n = 10)) and received, for 60 days, Phenofeed Dry® at 500 mg/cow/day. Milk and blood samples were collected before the start of the treatment (T0), subsequently every 15 days (T1–T4) and at 45 days after the end of treatment (T5). In the serum, glucose and triglycerides, stress, the thyroid, lactation and sex hormones were measured; in the milk, lysozyme content as well as the fatty acid profile were assessed. In the whole animal, the enriched feed helped to maintain hormonal parameters in the physiological range while producing hypoglycemic (T4 vs. T0, for P and PL p < 0.001) and hypolipidemic effects (T4 vs. T0, for P p < 0.001 and for PL p < 0.01). At the milk level, it resulted in a reduction in total fat (T5 vs. T0, for P, S and PL p < 0.001) and in the saturated fatty acids (SFAs)/monounsaturated fatty acids (MUFAs) ratio paralleled by an increase in polyunsaturated fatty acids (PUFAs) (T5 vs. T0, for P, S and PL p < 0.001), protein content (lysozyme (T4 vs. T0, for P and PL p < 0.001)) and lactose (T5 vs. T0, for P, S and PL p < 0.001). Thus, the inclusion of natural bioactive molecules such as O. europaea L. polyphenols in the dairy cow diet may help to improve animal welfare and milk quality.
Serum Allergen-Specific Immunoglobulin E in Cats with Inflammatory Bronchial Disease
Authors: Hörner-Schmid Lina , Palić Jelena , Mueller Ralf S. , Schulz Bianka
Published: October 2023

The etiology of feline inflammatory bronchial disease is poorly understood. This study compares the degree of allergen-specific serum IgE responses between cats with feline asthma, chronic bronchitis, mixed inflammation, and clinically healthy cats (HCs). The retrospective case–control study used serum from eighteen cats with eosinophilic inflammation (EI), ten with neutrophilic inflammation (NI), six with mixed inflammation (MI), and fourteen HCs. Affected cats were categorized into groups based on bronchoalveolar lavage cytology. The measurement of IgE for 34 different allergens including fungal organisms, weeds, grasses, trees, mites, and insects was performed using an indirect ELISA. Positive reactions to allergens were detected in the serum of 17/18 cats with EI, 8/10 with NI, 6/6 with MI, and 11/14 HCs (p = 0.364). When overall positive reactions were compared between groups, cats with MI (p = <0.01) had significantly more positive reactions against mite allergens than HCs. Blood eosinophils inversely correlated with the absolute amount of allergen-specific serum IgE expressed in ELISA absorbance units (EAs) (p = 0.014). Sensitization against dust mites seems to be more prevalent in cats with MI. However, positive IgE reactions can be observed in healthy and diseased cats, and, therefore, need to be interpreted in the light of clinical findings and environmental conditions of individual patients.
Extended Versus Intermittent Meropenem Infusion in the Treatment of Nosocomial Pneumonia: A Retrospective Single-Center Study
Authors: Hyun Dong-gon , Seo Jarim , Lee Su Yeon , Ahn Jee Hwan , Hong Sang-Bum , Lim Chae-Man , Koh Younsuck , Huh Jin Won
Published: October 2023

The efficacy of extended meropenem infusions in patients with nosocomial pneumonia is not well defined. Therefore, we compared the clinical outcomes of extended versus intermittent meropenem infusions in the treatment of nosocomial pneumonia. We performed a retrospective analysis of extended versus intermittent meropenem infusions in adult patients who had been treated for nosocomial pneumonia at a medical ICU between 1 May 2018 and 30 April 2020. The primary outcome was mortality at 14 days. Overall, 64 patients who underwent an extended infusion and 97 with an intermittent infusion were included in this study. At 14 days, 10 (15.6%) patients in the extended group and 22 (22.7%) in the intermittent group had died (adjusted hazard ratio (HR), 0.55; 95% confidence interval (CI): 0.23–1.31; p = 0.174). In the subgroup analysis, significant differences in mortality at day 14 were observed in patients following empirical treatment with meropenem (adjusted HR, 0.17; 95% CI: 0.03–0.96; p = 0.045) and in Gram-negative pathogens identified by blood or sputum cultures (adjusted HR, 0.01; 95% CI: 0.01–0.83; p = 0.033). Extended infusion of meropenem compared with intermittent infusion as a treatment option for nosocomial pneumonia may have a potential advantage in specific populations.
Extraction of Bioactive Compounds from Prestonia mollis Leaves and Their Impregnation into Polylactic Acid Using High-Pressure Technologies: Potential for Biomedical Application
Authors: Burgos-Briones Gabriel Alfonso , Verano-Naranjo Lidia , Cejudo-Bastante Cristina , Dueñas-Rivadeneira Alex Alberto , Mantell-Serrano Casimiro , Casas-Cardoso Lourdes
Published: October 2023

Enhanced solvent extraction (ESE) and pressurized liquid extraction (PLE) have been used for the first time to obtain antioxidant compounds from Prestonia mollis leaves. The effects of pressure (100–250 bar), temperature (55–75 °C) and the composition of the extraction solvent (ethanol, water and hydroalcoholic mixtures) were evaluated according to multilevel factorial designs. PLE provided the largest extraction yields compared to ESE, as well as a greater impact of the operating conditions studied. The highest total phenolic content was obtained when using a hydroalcoholic mixture (CO2/ethanol/water 50/25/25) through ESE at 100 bar and 75 °C. The antioxidant capacity of this extract is related to higher concentration levels of the identified flavonoids: Quercetin 3-O-xylosyl-rutinoside, Kaempferol 3-(2G-apiosylrobinobioside) and Kaempferol 4′-glucoside 7-rhamnoside. This extract was tested for the supercritical impregnation of polylactic acid (PLA), which is a polymer widely used in the biomedical industry. The influence of pressure (100–400 bar), temperature (35–55 °C), amount of extract (3–6 mL) and impregnation time (1–2 h) have been evaluated. The best results were obtained by impregnating 3 mL of extract at 100 bar and 55 °C for 2 h, achieving 10% inhibition with DPPH methods. The extract presented a potentially suitable impregnation of PLA for biomedical applications.
Characterization of the Metallothionein Gene Family in Avena sativa L. and the Gene Expression during Seed Germination and Heavy Metal Stress
Authors: Konieczna Wiktoria , Mierek-Adamska Agnieszka , Chojnacka Natalia , Antoszewski Marcel , Szydłowska-Czerniak Aleksandra , Dąbrowska Grażyna B.
Published: October 2023

Metallothioneins (MTs) are a family of small proteins rich in cysteine residues. The sulfhydryl group of metallothioneins can bind to metal ions, maintaining metal homeostasis and protecting the cells from damage caused by toxic heavy metals. Moreover, MTs can function as reactive oxygen species scavengers since cysteine thiols undergo reversible and irreversible oxidation. Here, we identified 21 metallothionein genes (AsMTs) in the oat (Avena sativa L.) genome, which were divided into four types depending on the amino acid sequences of putative proteins encoded by identified genes. Analysis of promoter sequences showed that MTs might respond to a variety of stimuli, including biotic and abiotic stresses and phytohormones. The results of qRT-PCR showed that all four types of AsMTs are differentially expressed during the first 48 hours of seed germination. Moreover, stress induced by the application of zinc, cadmium, and a mixture of zinc and cadmium affects the expression of oat MTs variously depending on the MT type, indicating that AsMT1-4 fulfil different roles in plant cells.
Improving Adversarial Robustness via Distillation-Based Purification
Authors: Koo Inhwa , Chae Dong-Kyu , Lee Sang-Chul
Published: October 2023

Despite the impressive performance of deep neural networks on many different vision tasks, they have been known to be vulnerable to intentionally added noise to input images. To combat these adversarial examples (AEs), improving the adversarial robustness of models has emerged as an important research topic, and research has been conducted in various directions including adversarial training, image denoising, and adversarial purification. Among them, this paper focuses on adversarial purification, which is a kind of pre-processing that removes noise before AEs enter a classification model. The advantage of adversarial purification is that it can improve robustness without affecting the model’s nature, while another defense techniques like adversarial training suffer from a decrease in model accuracy. Our proposed purification framework utilizes a Convolutional Autoencoder as a base model to capture the features of images and their spatial structure.We further aim to improve the adversarial robustness of our purification model by distilling the knowledge from teacher models. To this end, we train two Convolutional Autoencoders (teachers), one with adversarial training and the other with normal training. Then, through ensemble knowledge distillation, we transfer the ability of denoising and restoring of original images to the student model (purification model). Our extensive experiments confirm that our student model achieves high purification performance(i.e., how accurately a pre-trained classification model classifies purified images). The ablation study confirms the positive effect of our idea of ensemble knowledge distillation from two teachers on performance.
Influences of Sex on Muscle Architecture and Performance in Elite Field Hockey Players
Authors: Bartolomei Sandro , D’Amico Alessio , Treno Filippo , Cortesi Matteo , Pagliara Stefano , Mignardi Sergio
Published: October 2023

The aim of this study was to compare muscle architecture and performance between male and female elite Field Hockey players and to investigate the relationships between echo intensity and performance. Twenty-one male (24.3 ± 3.6 y; 75.1 ± 8.5 kg; 176.8 ± 6.4 cm) and nineteen female players (27.4 ± 3.9 y; 61.2 ± 7.4 kg; 164.4 ± 4.9 cm) were tested for muscle thickness (MT) and echo intensity (EI) of trapezius (Trap) and vastus lateralis (VL) muscles. Participants were also assessed for bench press power, and 30 m sprint. Results showed a higher VLMT and TrapMT in male players compared to female players (+22.1%; p = 0.004 and +25.8%; p = 0.001 for VLMT and TrapMT, respectively). A lower VLEI was detected in male players compared to female players (−20.7%; p = 0.001), while no significant differences were detected for TrapEI. Male players were faster than female players in a 30 m sprint and more powerful at the bench press (p < 0.001). Significant correlations were detected between VLEI and 30 m sprint (r = 0.74) in female players only. Results indicate that differences exist between male and female elite Field Hockey players in the EI of lower body muscles, while these differences are not present in the upper body muscles. EI, together with other sprint and power assessments, may represent an important parameter for elite Field Hockey players.
Stress Measurements of GIS Epoxy Composite with Transverse Waves Based on the Ultrasonic Pulse-Echo Method
Authors: Zou Zhouyiao , Qiu Haian , Tian Fangyuan , Kuang Cuizhe , He Weiming , Zhou Fusheng , Gu Yu , Ma Haomin , Ruan Youde , Huang Zhiming
Published: October 2023

The mechanical reliability of the gas-insulated metal-enclosed switchgear (GIS) is critical to the safe and stable operation of a power system. The internal stress induced in GIS insulators, which typically lead to cracking, can degrade the mechanical properties and compromise the reliability of the GIS. In this work, ultrasonic nondestructive measurements of the internal stress in a GIS 126 kV epoxy composite with the transverse waves are demonstrated for the first time. Our results show that the velocities of ultrasonic transverse waves increase linearly with the compressive stress and decrease linearly with the tensile stress. The acoustoelastic coefficients are −1.18 × 10−4/MPa and −1.96 × 10−4/MPa for measurements with transverse waves polarized vertically and parallelly to stress, respectively. Based on these acoustoelastic coefficients, the internal stresses are calculated, and the maximum absolute values of relative errors are 35.4% and 26.3% for measurements with the transverse wave polarized vertically and parallelly to stress, respectively.