bluuebunny/crossref_metadata_2025 huggingface dataset:
Inverter-Less Integration of Roof-Top Solar PV with Grid Connected Industrial Drives
Authors: Khan M. Ryyan , Alam Intekhab , Khan M. Rezwan
Published: February 2023

Green energy from Solar PV is getting increased attention in the industries due to the falling price of solar panels in the world market. A grid-tied inverter is one of the major components in such a system, where the DC energy from PV is converted to AC and synchronized with the grid to obtain power sharing between the PV and the grid for the industrial drives. In this paper, a DC link has been proposed instead of an AC link for interconnection between the solar PV system and the grid to run those industrial drives. In most modern industrial applications, induction motors are driven by VVVF (Variable Voltage and Variable Frequency) inverters to achieve efficient speed control. The inverters commonly have a rectifier section at the front end that rectifies the input AC to DC and the DC is then used in PWM mode to generate the required voltage and frequency for the induction motor operating under variable speed and load conditions. Such an inverter can use both AC or DC as the input so long the supply voltage has the right value for the inverter to operate. In our proposition, we eliminate the grid-tied inverter and use a DC link, created from the rectified AC and the regular Solar PV, to obtain the power-sharing between the PV output and the grid. Using the DC link output directly to energize the VVVF inverter has an impact on the performance of the inverter. In the proposed system, the solar PV array is designed in such a way that the grid remains as the supplementary power source only to supplement any shortfall in the PV output due to variable sunshine conditions. The control circuit used in this novel technique is inexpensive, efficient, and simple in design when compared to the grid-tied inverters. The proposed system has been implemented at Niagara Textiles in Gazipur, Bangladesh. The experimental/practical results are presented to validate the basic concept. Around a 20% reduction in the cost of energy has been reported in this paper, with a more than 90% efficient system. This will definitely make solar PV energy more competitive with regular energy and attractive to industries for its simplicity.
Energy Efficiency of Tall Buildings: A Global Snapshot of Innovative Design
Authors: Ali Mir M. , Al-Kodmany Kheir , Armstrong Paul J.
Published: February 2023

Design priorities for tall and supertall buildings have for some time shifted to achieving more energy efficiency to address the energy needs of the increasing global population. Engineers and architects aim to achieve energy conservation through active and passive approaches, pursuing technological innovations and adopting climate-responsive design. Because of the green movement currently dominating the building industry, tall buildings that need a massive amount of energy to build and operate, and the practical desire to switch from non-renewable to clean renewable energy resources, intense attention has been given to the energy efficiency of tall buildings in the recent past. Due to the vast array of energy-efficient design features, equipment, and applications available now, it is timely to examine the pros and cons of these issues. This review paper is an attempt to comprehensively present and deliberate these issues. It illustrates and discusses the concepts and applications through a few case studies from several continents worldwide. The review shows that the design of tall buildings focusing on energy conservation is an evolutionary process and there is a need for further research about how to face the associated challenges to improve energy efficiency by developing creative solutions and strategies, as well as applying additional innovative technologies.
Chromium in Water and Carcinogenic Human Health Risk
Authors: Georgaki Maria-Nefeli , Charalambous Marianna , Kazakis Nerantzis , Talias Michael A. , Georgakis Charalampos , Papamitsou Theodora , Mytiglaki Christina
Published: February 2023

Understanding the extent of human health risks with an emphasis on carcinogenesis development attributable to potentially toxic chemicals is critical to effective prevention and mitigation strategies. Chromium (Cr), mainly the hexavalent chromium (Cr (VI)), is a chemical associated with cancer when found in drinking water, making it a major public health issue. This study assessed a possible carcinogenic human health risk among the general population due to exposure to total or hexavalent chromium. We performed a systematic review of the international scientific literature, according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) protocol to determine the human risk of cancer mortality and morbidity. In total, 76 articles were checked for eligibility, 13 of which were included in the final systematic review. Only scientific articles from January 2000 to November 2022 published on PubMed were included. Data from both epidemiological ecological studies (Relative Risk and Rate Ratio—RR and Standardized Mortality Rate—SMR) and epidemiological case studies (Lifetime Cancer Risk—LCR, Incremental Lifetime Cancer Risk—ILCR, Cancer Risk—CR, Hazard Quotient—HQ, Hazard Index—HI, Health Risk Assessment—HRA, Disability-Adjusted Life Year—DALY, and Chronic Daily Intake Index—CDI) were included for the overall assessment of carcinogenicity in the general population. According to most articles, there is credible evidence that hexavalent chromium via water is indicated as a major contributor to the global burden of cancer in humans. Some of them emphasize malignant neoplasms in the lung, liver, stomach, and genitourinary system. Although the health index data of the case studies are based on a limited number of samples, they raise concerns about the possibility of an increase in the degree of carcinogenesis. However, there are significant limitations due to the lack of information on the dose and duration of exposure in the target group. Further research involving extensive analysis of the association of the two variables is needed, which depends on more complete information extraction and advanced methodologies.
Epigenetic Regulation in Breast Cancer: Insights on Epidrugs
Authors: Kim Ayoung , Mo Kyumin , Kwon Hyeonseok , Choe Soohyun , Park Misung , Kwak Woori , Yoon Hyunho
Published: February 2023

Breast cancer remains a common cause of cancer-related death in women. Therefore, further studies are necessary for the comprehension of breast cancer and the revolution of breast cancer treatment. Cancer is a heterogeneous disease that results from epigenetic alterations in normal cells. Aberrant epigenetic regulation is strongly associated with the development of breast cancer. Current therapeutic approaches target epigenetic alterations rather than genetic mutations due to their reversibility. The formation and maintenance of epigenetic changes depend on specific enzymes, including DNA methyltransferases and histone deacetylases, which are promising targets for epigenetic-based therapy. Epidrugs target different epigenetic alterations, including DNA methylation, histone acetylation, and histone methylation, which can restore normal cellular memory in cancerous diseases. Epigenetic-targeted therapy using epidrugs has anti-tumor effects on malignancies, including breast cancer. This review focuses on the importance of epigenetic regulation and the clinical implications of epidrugs in breast cancer.
Integrative Analysis of the Identified Transcriptome and Proteome Major Metabolism Pathways Involved in the Development of Grafted Apricot Hybrids
Authors: Sun Xiying , Tian Li , Xu Wanyu , Feng Luying , Jia Wenqing , Liu Yiteng , Chen Zhuo , Zhang Shulin , Zhang Xianliang , Ru Guangxin
Published: February 2023

Plant distant grafting can produce stable genetic variation, which is a new method for germplasm innovation. Two chimeras, peach/apricot (PA) and apricot/peach (AP), were created through two-way grafting between peach and apricot. The leaves, flowers and fruit phenotypes of chimeras were significantly different to self-rooted rootstock. In order to investigate the causes of such changes, transcriptome and proteome integrative analyses were conducted on apricots from these two chimeras. Many differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) that may be connected to the development of grafted apricot hybrids were identified and explored based on function. Moreover, we found 76 genes in forward-grafted PA and 46 in reverse-grafted AP that overlapped both in DEGs and DEPs (DEGs/DEPs) via transcriptome–proteome integrative analysis. Mapping the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database in PA and AP, the top significant enrichment pathways of DEGs/DEPs included lipid metabolism (fatty acid elongation, cutin, suberine and wax biosynthesis, fatty acid degradation and alpha-linolenic acid metabolism) and carbohydrate metabolism (glycolysis/gluconeogenesis, starch and sucrose metabolism and galactose metabolism), revealing that lipid metabolism and carbohydrate metabolism may play an irreplaceable role in the development of grafted apricot hybrids. Taken together, this work uncovered numerous candidate transcripts and proteins involved in the development of grafted apricot hybrids. The molecular mechanisms provide new insights into this important process in other heterografting hybrids.
Ingestion of Species-Specific dsRNA Alters Gene Expression and Can Cause Mortality in the Forest Pest, Ips calligraphus
Authors: Wallace Mary , Rieske Lynne K.
Published: February 2023

Ips calligraphus (Germar) is a conifer pest that causes economically and ecologically significant tree mortality, particularly when forests are stressed. As forests become increasingly vulnerable to pest outbreaks due to habitat fragmentation, invasive species, or climate change, innovative management strategies are needed to augment traditional approaches. Manipulating the RNA interference (RNAi) pathway is emerging as a novel pest management technology that could serve as a means of managing I. calligraphus while minimizing non-target effects. Demonstrating effectiveness of exogenous double-stranded RNA (dsRNA) in inducing changes in gene expression and causing mortality is an essential step. In this study, oral ingestion of dsRNA caused significant changes in gene expression and increased mortality for two of the three target dsRNAs tested. Additionally, we sequenced 5 mRNA libraries from adult beetles to assemble a transcriptome, from which we identified sequences of target genes for dsRNAs, and 10 genes in the I. calligraphus transcriptome putatively involved in the RNAi pathway. We demonstrate that oral ingestion of exogenous dsRNA can trigger the RNAi pathway. This is the first published study to artificially trigger the RNAi pathway in an Ips spp. and the first step in evaluating the potential for pest management strategies utilizing RNAi against this pest.
An Agent-Based Model of a Sustainable Forest Operation in a Theoretical Lowland Dipterocarp Forest Modeled after Mount Makiling Forest Reserve, Philippines
Authors: Arnejo Zenith , Barua Leonardo , Ramirez Paul Joseph , Tiburan Jr. Cristino , Bantayan Nathaniel
Published: February 2023

This study investigates the integration of assisted natural regeneration (ANR) and selective logging (SL) to guarantee a sustainable forest operation in the Philippines using agent-based modeling. To assess the sustainability of the operation in light of the revenue from timber harvesting and the health of the forest in terms of the total number of trees, various simulations were run on a theoretical forest modeled after the Mount Makiling Forest Reserve in the Philippines. The findings of the simulation have shown that, even after many years of continuous use, the performance of SL on a healthy forest similar to the theoretical forest is substantially identical with and without ANR. The “with ANR” setup, however, was able to demonstrate a considerably better and more stable harvest value over the final 100 years than the “without ANR” setup. In terms of ensuring sustainable forest cover, simulation findings showed that even after 500 years of continuous SL activity, the forest cover could be maintained to up to 80% with ANR. The model has shown that with the right combination of reforestation efforts and timber harvesting methods, a sustainable forest operation can contribute to the country’s economic needs for timber production while ensuring that the forest is actively managed.
Optimizing Fuel Treatments Allocation to Protect the Wildland–Urban Interface from Large-Scale Wildfires in Greece
Authors: Bachantourian Margarita , Kalabokidis Kostas , Palaiologou Palaiologos , Chaleplis Kyriakos
Published: February 2023

A crucial risk governance priority of the Greek forest managers is to reduce damages in the wildland–urban interface (WUI) by controlling wildfire behavior through fuel management practices. To support decisions for where management should be applied and how, this study experimented with new methods for fuel treatments allocation over a typical Mediterranean fire-prone landscape in the peninsula of Kassandra (an area of 350 km2), northern Greece. The Minimum Travel Time (MTT) fire simulation algorithm and the Treatment Optimization Model were used to produce eight spatial exclusionary and non-exclusionary datasets that were used as criteria for the spatial optimization of fuel management interventions. We used the Multicriteria Decisions Analysis method with Geographical Information Systems to cartographically intersect the criteria to produce two priority maps for two forest management scenarios (i.e., a control and a realistic one). The results revealed that 48 km2 of the study area was characterized as high-priority locations in the control scenario (i.e., with equally weighted management priorities), while 60 km2 was assigned to the high-priority class in the realistic scenario (i.e., with different weighted management priorities). Further analysis showed a substantial variation in treatment priority among the four major forest land cover types (broadleaves, sparse Mediterranean shrublands, conifers, and dense Mediterranean shrublands), revealing that the latter two had the highest selection values. Our methodological framework has already been operationally used by the Greek Forest Service branch of Kassandra to decide the most effective landscape fuel treatment allocation.
Behavior and Capacity of Moment-Frame Members and Connections during Fire
Authors: Chinivar Supriya N. , Sener Kadir C.
Published: February 2023

This paper focuses on investigating the structural behavior of members and connections that are part of moment frames under the combined effect of bending moment and thermally induced axial force during a compartment-fire event. The finite-element analysis method was employed to conduct this study using models benchmarked against experimental data from several past studies while utilizing temperature-dependent material models. A numerical parametric study on typical floor beams with slender elements for compression were conducted under combined bending and axial loading to develop interaction-capacity curves at temperatures representing fire events. The results were compared against the member-strength equations provided in the AISC Specification. The analysis results demonstrated that the AISC beam-column strength equations including the combined effects of axial-load and bending moment provided reasonable estimates for member-slenderness ratios greater than 60, but overestimated the strength of beams with slender elements for low member-slenderness ratios. Combined-load-strength studies were also conducted on a typical connection used in moment frames. The moment-connection behavior was governed by failure modes exhibited at the ends of floor beams. Therefore, the interaction equations available for beam columns resulted in conservative estimates and are recommended for calculating moment-connection capacity during compartment-fire scenarios.
Editing the Melanocortin-4 Receptor Gene in Channel Catfish Using the CRISPR-Cas9 System
Authors: Khalil Karim , Elaswad Ahmed , Abdelrahman Hisham , Michel Maximillian , Chen Wenbiao , Liu Shikai , Odin Ramjie , Ye Zhi , Drescher David , Vo Khoi , Bugg William S. , Qin Guyu , Yang Yujia , Backenstose Nathan J. C. , Liu Zhanjiang , Cone Roger D. , Dunham Rex
Published: February 2023

The melanocortin-4 receptor (MC4R) plays a critical role in homeostasis and the regulation of body weight. Polymorphisms in the mc4r gene have been discovered and linked to growth, carcass composition, and meat quality traits. Therefore, we used the CRISPR-Cas9 system to target the mc4r gene in the most important freshwater aquaculture species in the USA, channel catfish, Ictalurus punctatus. Guide RNAs were designed to direct the Cas9 to the coding sequence of the channel catfish mc4r gene. gRNA(s)-Cas9 mixtures were delivered into one-cell embryos using electroporation and microinjection. For each treatment, the nature and rate of mutations were analyzed. Hatching and survival rates were calculated. The overall mutation rates were 30.6% and 66.7–90.6% for electroporation and microinjection, respectively. Mutated fish generated via electroporation or microinjection exhibited 38% and 20% improvement in body weight, respectively, when compared with the full-sib control. The mean feed conversion ratio of the mutants was 1.18 compared with 1.57 in the control fish. The improved growth and feed conversion indicate that the generation of mc4r-edited fish could economically benefit aquaculture production.
CRISPR/Cas9-Mediated Disruption of Endo16 Cis-Regulatory Elements in Sea Urchin Embryos
Authors: Xing Lili , Wang Lingyu , Roos Femke , Lee Michelle , Wray Gregory A.
Published: February 2023

Sea urchins have become significant mariculture species globally, and also serve as invertebrate model organisms in developmental biology. Cis-regulatory elements (enhancers) control development and physiology by regulating gene expression. Mutations that affect the function of these sequences may contribute to phenotypic diversity. Cis-regulatory targets offer new breeding potential for the future. Here, we use the CRISPR/Cas9 system to disrupt an enhancer of Endo16 in developing Lytechinus variegatus embryos, in consideration of the thorough research on Endo16’s regulatory region. We designed six gRNAs against Endo16 Module A (the most proximal region of regulatory sequences, which activates transcription in the vegetal plate and archenteron, specifically) and discovered that Endo16 Module A-disrupted embryos failed to undergo gastrulation at 20 h post fertilization. This result partly phenocopies morpholino knockdowns of Endo16. Moreover, we conducted qPCR and clone sequencing experiments to verify these results. Although mutations were not found regularly from sequencing affected individuals, we discuss some potential causes. In conclusion, our study provides a feasible and informative method for studying the function of cis-regulatory elements in sea urchins, and contributes to echinoderm precision breeding technology innovation and aquaculture industry development.
Untargeted Metabolite Profiling of Adipose Tissue in Rats Exposed to Mepiquat
Authors: Hu Chuanqin , Song Xinyu , Shao Zhenzhen , Liu Yingli , Wang Jing , Sun Baoguo
Published: February 2023

Mepiquat (Mep) is a contaminant produced by Maillard reaction with reducing sugar, free lysine and an alkylating agent under typical roasting conditions, particularly in the range of 200–240 °C. It has been reported that exposure to Mep is harmful to rats. However, its metabolic mechanism is still not clear. In this study, untargeted metabolomics was used to reveal the effect of Mep on the metabolic profile of adipose tissue in Sprague-Dawley rats. Twenty-six differential metabolites were screened out. Eight major perturbed metabolic pathways were found, which were linoleic acid metabolism, Phenylalanine, tyrosine, and tryptophan biosynthesis, phenylalanine metabolism, arachidonic acid metabolism, Glycine, serine, and threonine metabolism, glycerolipid metabolism, Alanine, aspartate, and glutamate metabolism, and glyoxylate and dicarboxylic acid metabolism. This study lays a solid foundation for clarifying the toxic mechanism of Mep.
Improving Pea Protein Emulsifying Capacity by Glycosylation to Prepare High-Internal-Phase Emulsions
Authors: Morell Pere , López-García Adrián , Hernando Isabel , Quiles Amparo
Published: February 2023

Pea protein has been extensively studied because of its high nutritional value, low allergenicity, environmental sustainability, and low cost. However, the use of pea protein in some food products is hindered due to the low functionality of pea protein, especially as an emulsifier. High-internal-phase emulsions (HIPEs) are attracting attention because of their potential application in the replacement of hydrogenated plastic fats in foods. In this study, the use of glycated pea protein isolate (PPI) as an emulsifier to prepare HIPEs is proposed. The functionalization of a commercial PPI in two ratios of maltodextrin (MD) (1:1 and 1:2) via glycosylation (15 and 30 min), to act as an emulsifier in HIPEs, is investigated. HIPE properties, such as oil loss and texture, were evaluated and related to microstructural properties. Glycated-PPI-stabilized HIPEs showed high consistency, firmness, viscosity, and cohesiveness values; a tight and homogeneous structure; and physical stability throughout storage. The results showed that emulsions were more stable when using a 1:2 ratio and 30 min of heat treatment. However, the reaction time was more determinant for improving the textural properties when a 1:1 ratio was used for glycosylation than when a 1:2 ratio was used. Glycosylation with MD via the Maillard reaction is a suitable method to enhance the emulsifying and stabilizing properties of PPI.
Polyphenol and Flavonoid Stability of Wild Blueberry (Sideroxylon mascatense) during Air- and Freeze-Drying and Storage Stability as a Function of Temperature
Authors: Al Hasani Shaima , Al-Attabi Zahir , Waly Mostafa , Al-Habsi Nasser , Al-Subhi Lyutha , Shafiur Rahman Mohammad
Published: February 2023

Būt (Sideroxylon mascatense) is an indigenous wild blueberry found in Oman. It has a very short season and is commonly preserved by drying. The aims of this study were to determine the physico-chemical characteristics and stability of phytochemicals (i.e., polyphenols and flavonoids) in the berries during drying (i.e., freeze-drying at −40 °C and air-drying at 60 and 90 °C) and the polyphenol stability of the dried berries as a function of storage temperature (i.e., 90, 70, 60, 40, 20, and −20 °C). The moisture content of fresh berry flesh was 64.5 g/100 g sample (wet basis). The crude protein and fat contents were higher in the seeds than in the flesh. Glucose and fructose were the main sugars and their concentrations were highest in the sample air-dried at 60 °C. The initial total polyphenol content (TPC) and total flavonoid content (TFC) of the flesh were 2.009 mg gallic acid equivalent (GAE)/g dry-solids and 0.199 mg catechin equivalent (CE)/g dry-solids, respectively. The samples air-dried at 90 °C and freeze-dried at −40 °C had higher TPC (i.e., 2.638 mg GAE/g dry-solids) and TFC (i.e., 0.395 mg CE/g dry-solids), respectively. There was a significant difference between the TPC and TFC of fresh and dried wild berries (p < 0.05). The freeze-dried wild berries retained a high TPC compared to the air-dried samples. The polyphenol storage stability of freeze-dried wild berries at different storage temperatures showed two phases: an initial release phase followed by a decay phase. The polyphenol storage stability was modeled using the Peleg model and the kinetic parameters were correlated with the storage temperature.
Estimation of Impacts of Global Factors on World Food Prices: A Comparison of Machine Learning Algorithms and Time Series Econometric Models
Authors: Ulussever Talat , Ertuğrul Hasan Murat , Kılıç Depren Serpil , Kartal Mustafa Tevfik , Depren Özer
Published: February 2023

It is a well-felt recent phenomenal fact that global food prices have dramatically increased and attracted attention from practitioners and researchers. In line with this attraction, this study uncovers the impact of global factors on predicting food prices in an empirical comparison by using machine learning algorithms and time series econometric models. Covering eight global explanatory variables and monthly data from January 1991 to May 2021, the results show that machine learning algorithms reveal a better performance than time series econometric models while Multi-layer Perceptron is defined as the best machine learning algorithm among alternatives. Furthermore, the one-month lagged global food prices are found to be the most significant factor on the global food prices followed by raw material prices, fertilizer prices, and oil prices, respectively. Thus, the results highlight the effects of fluctuations in the global variables on global food prices. Additionally, policy implications are discussed.
Impact of Starch-Rich Food Matrices on Black Rice Anthocyanin Accessibility and Carbohydrate Digestibility
Authors: Ou Sean Jun Leong , Fu Amanda Simin , Liu Mei Hui
Published: February 2023

Anthocyanins reduce starch digestibility via carbohydrase-inhibitory pathways, but food matrix effects during digestion may also influence its enzymatic function. Understanding anthocyanin-food matrix interactions is significant as the efficiency of carbohydrase inhibition relies on anthocyanin accessibility during digestion. Therefore, we aimed to evaluate the influence of food matrices on black rice anthocyanin accessibility in relation to starch digestibility in common settings of anthocyanin consumption—its co-ingestion with food, and consumption of fortified food. Our findings indicate that black rice anthocyanin extracts (BRAE) had reduced intestinal digestibility of bread to a larger extent for the co-digestion of BRAE with bread (39.3%) (4CO), than BRAE-fortified bread (25.9%) (4FO). Overall anthocyanin accessibility was about 5% greater from the co-digestion with bread than fortified bread across all digestion phases. Differences in anthocyanin accessibility were also noted with changes to gastrointestinal pH and food matrix compositions—with up to 10.1% (oral to gastric) and 73.4% (gastric to intestinal) reductions in accessibility with pH changes, and 3.4% greater accessibility in protein matrices than starch matrices. Our findings demonstrate that the modulation of starch digestibility by anthocyanin is a combined result of its accessibility, food matrix composition, and gastrointestinal conditions.
Effects of Hevea brasiliensis Intercropping on the Volatiles of Pandanus amaryllifolius Leaves
Authors: Zhang Ang , Lu Zhiqing , Yu Huan , Zhang Yaoyu , Qin Xiaowei , Ji Xunzhi , He Shuzhen , Zong Ying , Zhong Yiming , Li Lihua
Published: February 2023

Pandanus amaryllifolius Roxb. is a special tropical spice crop resource with broad development prospects. It is widely cultivated under a Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. canopy to improve the comprehensive benefits to Hevea brasiliensis plantations in Hainan Provence, China. However, the effects of intercropping with Hevea brasiliensis on the component number and relative contents of volatile substances in different categories in the Pandanus amaryllifolius leaves are still unknown. Therefore, a Hevea brasiliensis and Pandanus amaryllifolius intercropping experiment was set up to clarify the differences between several cultivated patterns on volatile substances in the Pandanus amaryllifolius leaves, and the key regulatory factors of volatile substances. The results showed that the soil pH was significantly decreased, while soil bulk density, alkali-hydrolyzable nitrogen and available phosphorus contents were significantly increased under the intercropping pattern. The component numbers of esters in volatile substances were increased by 6.20%, while the component numbers of ketones were decreased by 4.26% under the intercropping pattern. Compared with the Pandanus amaryllifolius monoculture, the relative contents of pyrroles, esters and furanones were significantly increased by 8.83%, 2.30% and 8.27%, respectively, while the relative contents of ketones, furans and hydrocarbons were decreased by 1.01%, 10.55% and 9.16% under the intercropping pattern, respectively. The relative contents of pyrroles, esters, furanones, ketones, furans and hydrocarbons were associated with changes in soil pH, soil available phosphorus content and air temperature. The results indicated that the reduction in soil pH and enhancement in soil-available phosphorus may be the main reasons for promoting the relative content of pyrroles and reducing the relative content of hydrocarbons under an intercropping pattern. Overall, Hevea brasiliensis intercropping with Pandanus amaryllifolius could not only improve soil properties, but also significantly increase the relative contents of the main volatile substances in Pandanus amaryllifolius leaves, which could provide a theoretical basis for the application and promotion of high-quality production patterns of Pandanus amaryllifolius.
Disease Occurrence in- and the Transferal of Zoonotic Agents by North American Feedlot Cattle
Authors: Koyun Osman Y. , Balta Igori , Corcionivoschi Nicolae , Callaway Todd R.
Published: February 2023

North America is a large producer of beef and contains approximately 12% of the world’s cattle inventory. Feedlots are an integral part of modern cattle production in North America, producing a high-quality, wholesome protein food for humans. Cattle, during their final stage, are fed readily digestible high-energy density rations in feedlots. Cattle in feedlots are susceptible to certain zoonotic diseases that impact cattle health, growth performance, and carcass characteristics, as well as human health. Diseases are often transferred amongst pen-mates, but they can also originate from the environment and be spread by vectors or fomites. Pathogen carriage in the gastrointestinal tract of cattle often leads to direct or indirect contamination of foods and the feedlot environment. This leads to the recirculation of these pathogens that have fecal–oral transmission within a feedlot cattle population for an extended time. Salmonella, Shiga toxin-producing Escherichia coli, and Campylobacter are commonly associated with animal-derived foods and can be transferred to humans through several routes such as contact with infected cattle and the consumption of contaminated meat. Brucellosis, anthrax, and leptospirosis, significant but neglected zoonotic diseases with debilitating impacts on human and animal health, are also discussed.
Dynamics and Stability of a Fractional-Order Tumor–Immune Interaction Model with B-D Functional Response and Immunotherapy
Authors: Feng Xiaozhou , Liu Mengyan , Jiang Yaolin , Li Dongping
Published: February 2023

In this paper, we investigate a fractional-order tumor–immune interaction model with B-D function item and immunotherapy. First, the existence, uniqueness and nonnegativity of the solutions of the model are established. Second, the local and global asymptotic stability of some tumor-free equilibrium points and a unique positive equilibrium point are obtained. Finally, we use numerical simulation method to visualize and verify the theoretical conclusions. It is known that the fractional-order parameter β has a stabilization effect, and the tumor cells can be destroyed or controlled by using immunotherapy.
Some Local Fractional Hilbert-Type Inequalities
Authors: Vuković Predrag
Published: February 2023

The main purpose of this paper is to prove some new local fractional Hilbert-type inequalities. Our general results are applicable to homogeneous kernels. Furthermore, the best possible constants in terms of local fractional hypergeometric function are obtained. The obtained results prove that the employed method is very simple and effective for treating various kinds of local fractional Hilbert-type inequalities.
New Method to Detect and Characterize Active Be Star Candidates in Open Clusters
Authors: Granada Anahí , Ghoreyshi Maziar R. , Jones Carol E. , Eenmäe Tõnis
Published: February 2023

With the aim of better understanding the physical conditions under which Be stars form and evolve, it is imperative to further investigate whether poorly studied young open clusters host Be stars. In this work, we explain how data from Gaia DR2 and DR3 can be combined to recover and characterize active Be stars in open clusters. We test our methodology in four open clusters broadly studied in the literature, known for hosting numerous Be stars. In addition, we show that the disk formation and dissipation approach that is typically used to model long term Be star variability, can explain the observed trends for Be stars in a (GDR3-GDR2) versus GDR3 plot. We propose that extending this methodology to other open clusters, and, in particular, those that are poorly studied, will help to increase the number of Be candidates. Eventually, Be stars may eclipse binary systems in open clusters.
Development of a Polyherbal Topical Gel for the Treatment of Acne
Authors: Chellathurai Benedict Jose , Anburose Ramyadevi , Alyami Mohammad H. , Sellappan Mohan , Bayan Mohammad F. , Chandrasekaran Balakumar , Chidambaram Kumarappan , Rahamathulla Mohamed
Published: February 2023

The present work aimed to formulate and evaluate a polyherbal gel using Aloe barbadensis and extract of Vigna radiata for the treatment of acne, a disorder of the skin in which hair follicles and sebaceous glands are blocked, causing inflammation and redness of the skin. Aloe barbadensis pulp was collected and mixed with the extract of Vigna radiata and formulated into a gel using Carbopol 940, triethanolamine, and propylene glycol as the gelling agent, viscosity modifier, and pH modifier, respectively. The gel was evaluated for its antimicrobial properties against Staphylococcus aureus, Escherichia coli, and Candida albicans. Antimicrobial agents, such as gentamycin and fluconazole, were used as the standards. The developed formulation showed promising zone of inhibition. The gel was further evaluated for its physicochemical properties. The formulation showed a promising effect on acne together with the additive effect of Aloe barbadensis on skin.
Bioengineered Water-Responsive Carboxymethyl Cellulose/Poly(vinyl alcohol) Hydrogel Hybrids for Wound Dressing and Skin Tissue Engineering Applications
Authors: Capanema Nádia Sueli Vieira , Mansur Alexandra Ancelmo Piscitelli , Carvalho Isadora Cota , Carvalho Sandhra Maria , Mansur Herman Sander
Published: February 2023

The burden of chronic wounds is growing due to the increasing incidence of trauma, aging, and diabetes, resulting in therapeutic problems and increased medical costs. Thus, this study reports the synthesis and comprehensive characterization of water-responsive hybrid hydrogels based on carboxymethyl cellulose (CMC) and poly(vinyl alcohol) (PVA) using citric acid (CA) as the chemical crosslinking agent, with tunable physicochemical properties suitable to be applied as a wound dressing for soft tissue engineering applications. They were produced through an eco-friendly process under mild conditions. The hydrogels were designed and produced with flexible swelling degree properties through the selection of CMC molecular mass (Mw = 250 and 700 kDa) and degree of functionalization (DS = 0.81), degree of hydrolysis of PVA (DH > 99%, Mw = 84–150 kDa) associated with synthesis parameters, CMC/PVA ratio and extension of chemical crosslinking (CA/CMC:PVA ratio), for building engineered hybrid networks. The results demonstrated that highly absorbent hydrogels were produced with swelling degrees ranging from 100% to 5000%, and gel fraction from 40% to 80%, which significantly depended on the concentration of CA crosslinker and the presence of PVA as the CMC-based network modifier. The characterizations indicated that the crosslinking mechanism was mostly associated with the chemical reaction of CA carboxylic groups with hydroxyl groups of CMC and PVA polymers forming ester bonds, rendering a hybrid polymeric network. These hybrid hydrogels also presented hydrophilicity, permeability, and structural features dependent on the degree of crosslinking and composition. The hydrogels were cytocompatible with in vitro cell viability responses of over 90% towards model cell lines. Hence, it is envisioned that this research provides a simple strategy for producing biocompatible hydrogels with tailored properties as wound dressings for assisting chronic wound healing and skin tissue engineering applications.
Hydrogel Encapsulation of Genome-Engineered Stem Cells for Long-Term Self-Regulating Anti-Cytokine Therapy
Authors: Collins Kelsey H. , Pferdehirt Lara , Saleh Leila S. , Savadipour Alireza , Springer Luke E. , Lenz Kristin L. , Thompson Dominic M. , Oswald Sara J. , Pham Christine T. N. , Guilak Farshid
Published: February 2023

Biologic therapies have revolutionized treatment options for rheumatoid arthritis (RA) but their continuous administration at high doses may lead to adverse events. Thus, the development of improved drug delivery systems that can sense and respond commensurately to disease flares represents an unmet medical need. Toward this end, we generated induced pluripotent stem cells (iPSCs) that express interleukin-1 receptor antagonist (IL-1Ra, an inhibitor of IL-1) in a feedback-controlled manner driven by the macrophage chemoattractant protein-1 (Ccl2) promoter. Cells were seeded in agarose hydrogel constructs made from 3D printed molds that can be injected subcutaneously via a blunt needle, thus simplifying implantation of the constructs, and the translational potential. We demonstrated that the subcutaneously injected agarose hydrogels containing genome-edited Ccl2-IL1Ra iPSCs showed significant therapeutic efficacy in the K/BxN model of inflammatory arthritis, with nearly complete abolishment of disease severity in the front paws. These implants also exhibited improved implant longevity as compared to the previous studies using 3D woven scaffolds, which require surgical implantation. This minimally invasive cell-based drug delivery strategy may be adapted for the treatment of other autoimmune or chronic diseases, potentially accelerating translation to the clinic.
“Are Not Our Interests the Same?”: Black Protest, the Lost Cause, and Coalition Building in Readjuster Virginia
Authors: Barnes Bryant K.
Published: February 2023

Virginia’s Readjuster Party was the most successful interracial political coalition in the post-Reconstruction South. Initially arising from a conflict over the payment of Virginia’s massive public debt, the new party became a force of liberal reform and democracy in the Old Dominion. It represented an alternative path before Jim Crow segregation and disfranchisement became the norm. While the Readjusters have long interested historians, the significant work performed by Black Readjusters in building and sustaining the always-tenuous coalition has gone understudied. Knowing their white counterparts were anxious about interracial political alliances, Black Readjusters eased these anxieties by using the Lost Cause. Black Readjusters condemned carpetbaggers as corrupt and repurposed the myth of the faithful slave to strengthen the interracial coalition, press for their own demands, and demonstrate their status as true southerners. The strategy and its seeming contradictions succeeded in some cases and failed in others, and its ultimate effects remain unclear. By shifting focus to Black Readjusters’ coalition-building labors, this article centers Black political activism and challenges the presumptions scholars make about interracial politics and white supremacy.
Novel Haplotype in the HHEX Gene Promoter Associated with Body Length in Pigs
Authors: Luo Yabiao , Xu Qiao , Xue Mingming , Wang Yubei , Yang Xiaoyang , Chan Shuheng , Tang Qiguo , Wang Feng , Sun Ruiping , Chao Zhe , Fang Meiying
Published: February 2023

The screening of important candidate genes and the identification of genetic markers are important for molecular selection in the pig industry. The hematopoietically expressed homeobox (HHEX) gene plays an important role in embryonic development and organogenesis; however, the genetic variation and expression pattern of the porcine HHEX gene remains to be clarified. In this study, semiquantitative RT-PCR and immunohistochemistry results showed the specific expression of the HHEX gene in porcine cartilage tissues. A novel haplotype consisting of two SNPs rs80901185 (T > C) and rs80934526 (A > G) was detected in the promoter region of the HHEX gene. The expression of the HHEX gene was significantly higher in Yorkshire pigs (TA haplotype) than in Wuzhishan pigs (CG haplotype), and a population analysis showed that this haplotype was significantly associated with body length. An analysis subsequently revealed that the –586 to –1 bp region of the HHEX gene promoter showed the highest activity. Furthermore, we found that the activity of the TA haplotype was significantly higher than that of the CG haplotype by changing the potential binding of transcription factors YY1 and HDAC2. In summary, we conclude that the porcine HHEX gene may contribute to the breeding of pigs for body length traits.
Special Issue “Genomics of Stroke” 2022
Authors: Limborska Svetlana A. , Filippenkov Ivan B.
Published: February 2023

Stroke is one of the greatest medical threats to human health and quality of life in modern society [...]
Histological and Microscopic Analysis of Fats in Heart, Liver Tissue, and Blood Parameters in Experimental Mice
Authors: Basheer Sehrish , Malik Imran Riaz , Awan Fazli Rabbi , Sughra Kalsoom , Roshan Sadia , Khalil Adila , Iqbal Muhammad Javed , Parveen Zahida
Published: February 2023

The intake of various types and amounts of dietary fats influences metabolic and cardiovascular health. Hence, this study evaluated the impact of routinely consumed Pakistani dietary fats on their cardiometabolic impact. For this, we made four groups of mice, each comprising 5 animals: (1) C-ND: Control mice on a normal diet, (2) HFD-DG: High-fat diet mice on a normal diet plus 10% (w/w) desi ghee, (3) HFD-O: Mice on normal diet plus 10% (w/w) plant oil (4) HFD-BG: Mice on normal diet plus 10% (w/w) banaspati ghee. Mice were fed for 16 weeks, and blood, liver, and heart samples were collected for biochemical, histological, and electron microscopic analysis. The physical factors indicated that mice fed on HFD gained more body weight than the C-ND group. Blood parameters do not show significant differences, but overall, the glucose and cholesterol concentrations were raised in the mice fed with a fat-rich diet, with the highest concentrations in the HFD-BG group. The mice fed with HFD-BG and HFD-O had more lipid droplets in the liver, compared to HFD-DG and C-ND.
An Introduction and Overview of RON Receptor Tyrosine Kinase Signaling
Authors: Hunt Brian G. , Fox Levi H. , Davis James C. , Jones Angelle , Lu Zhixin , Waltz Susan E.
Published: February 2023

RON is a receptor tyrosine kinase (RTK) of the MET receptor family that is canonically involved in mediating growth and inflammatory signaling. RON is expressed at low levels in a variety of tissues, but its overexpression and activation have been associated with malignancies in multiple tissue types and worse patient outcomes. RON and its ligand HGFL demonstrate cross-talk with other growth receptors and, consequentially, positions RON at the intersection of numerous tumorigenic signaling programs. For this reason, RON is an attractive therapeutic target in cancer research. A better understanding of homeostatic and oncogenic RON activity serves to enhance clinical insights in treating RON-expressing cancers.
Characterization of NDM-5-Producing Escherichia coli Strains Isolated from Pediatric Patients with Bloodstream Infections in a Chinese Hospital
Authors: Huang Lili , Hu Hongye , Xu Chen , Zhou Mi , Li Yuanyuan , Li Yunbing , Wu Shuyan , Dong Ning
Published: February 2023

Escherichia coli (E. coli) bloodstream infections (BSIs) are among the most predominant causes of death in infants and children worldwide. NDM-5 (New Delhi Metallo-lactamase-5) is responsible for one of the main mechanisms of carbapenem resistance in E. coli. To analyze the phenotypic and genomic characteristics of NDM-5-producing E. coli from bloodstream infections (BSIs), a total of 114 E. coli strains was collected from a children’s hospital in Jiangsu province, China. Eight blaNDM-5-carrying E. coli strains were identified which were all carbapenem-resistant and carried diverse antimicrobial resistance genes apart from blaNDM-5. They belonged to six distinct sequence types (STs) and serotypes including one each for ST38/O7:H8, ST58/O?:H37, ST131/O25:H4, ST156/O11:H25 and ST361/O9:H30 and three strains are originating from a single clone belonging to ST410/O?:H9. Apart from blaNDM-5, the E. coli strains isolated from BSIs also carried other β-lactamase genes, including blaCMY-2 (n = 4), blaCTX-M-14 (n = 2), blaCTX-M-15 (n = 3), blaCTX-M-65 (n = 1), blaOXA-1 (n = 4) and blaTEM-1B (n = 5). The blaNDM-5 genes were located on three different types of plasmids, which were IncFII/I1 (n = 1), IncX3 (n = 4) and IncFIA/FIB/FII/Q1 (n = 3). The former two types were conjugatively transferable at frequencies of 10−3 and 10−6, respectively. The dissemination of NDM-producing strains, which exhibit resistance to the last-line antibiotics, carbapenems, may increase the muti-antimicrobial resistance burden among E. coli BSIs and further threaten public health.
In Situ Simulation Training for Frailty
Authors: Dunnell Liam , Barnard Anna Nicole , Chu Katie , Barling Ania , Birns Jonathan , Walker Grace
Published: February 2023

Background: People living with frailty account for a significant proportion of hospital inpatients and are at increased risk of adverse events during admission. The understanding of frailty remains variable among hospital staff, and there is a need for effective frailty training across multidisciplinary teams. Simulation is known to be advantageous for improving human factor skills in multidisciplinary teams. In situ simulation can increase accessibility and promote ward team learning, but its effectiveness with respect to frailty has not been explored. Method: A single-centre, multi-fidelity, inter-professional in situ frailty simulation programme was developed. One-hour sessions were delivered weekly using frailty-based clinical scenarios. Mixed-method evaluation was used, with data collected pre- and post-session for comparison. Results: In total, 86 multidisciplinary participants attended 19 sessions. There were significant improvements in self-efficacy rating across 10 of 12 human factor domains and in all frailty domains (p < 0.05). The common learning themes were situational awareness, communication and teamwork. Participants commented on the value of learning within ward teams and having the opportunity to debrief. Conclusion: In situ simulation can improve the self-efficacy of clinical and human factor skills related to frailty. The results are limited by the nature of self-reporting methods, and further studies assessing behavioural change and clinical outcomes are warranted.