bluuebunny/crossref_metadata_2025 huggingface dataset:
Bench top CUT&RUN with antibodies-online™ CUT&RUN Sets v3
Authors: Online Gmbh Antibodies
Published: November 2019

CUT&RUN (Cleavage Under Targets and Release Using Nuclease) offers a novel approach to pursue epigenetics. The method is designed to map genome wide transcription factor binding sites, chromatin-associated complexes, and histone variants and post-translational modifications. Here we provide an updated CUT&RUN protocol that incorporates two variants, one is optimized to further reduce background noise which especially helps when working with low cell numbers and abundant antigens. In CUT&RUN is performed in situ on immobilized, intact cells without crosslinking. DNA fragmentation is achieved using micrococcal nuclease that is fused to Protein A and/or Protein G (pA/G-MNase). The fusion protein is directed to the desired target through binding of the Protein A/G moiety to the Fc region of an antibody bound to the target. DNA under the target is subsequently cleaved and released and the pA/G-MNase-antibody- chromatin complex is free to diffuse out of the cell. DNA cleavage products are extracted and then processed by next generation sequencing (NGS). All steps from live cells to sequencing-ready libraries can be performed in a single tube on the benchtop or a microwell in a high-throughput pipeline, and the entire procedure can be performed in one day.
Power in a single case multiple baseline design v1
Authors: Bouwmeester Samantha , Jongerling Joran
Published: November 2019

A randomization test can be used to statistically test hypotheses in multiple baseline designs to complement the commonly used visual inspection analysis. A crossed factor simulation study was performed to investigate the power of the Koehler and Levin (1998)randomization test in an multiple baseline design. The results show that the degree of autocorrelation of the observations, the number of participants, the effect size, the overlap of possible start moments of the intervention between participants, the ratio of the number of measurements in the baseline- and intervention phase, a gradually emerging effect, and the number of measurements had strong main effects on the power. The two-way interactions between number of participants and effect size, and between the number of measurements and the number of start moments of the intervention also had a large effect. An online tool was developed to calculate the power of a multiple baseline design given several design characteristics.
Marchantia Cryopreservation of Gemmae v1
Authors: Rebmann Marius , Tomaselli Marta , Silvestri Linda , Sauret-Gueto Susana , Lim Michelle
Published: November 2019

Simplified cryopreservation protocol for Marchantia polymorpha gemmae, based on (Tanaka et al. 2016, Plant and Cell Physiology). Enables long term storage of gemmae at -80°C.
Retahílas y canciones infantiles de procedencia europea en el exilio americano
Authors: Ruiz Fernández María Jesús , Pelegrín Graciela
Published: November 2019

La memoria infantil del exilio español se puede encontrar en los repertorios publicados en América a partir de 1939. Estas colecciones revelan diversos procesos de re-creación que ocurren en el flujo migratorio (a veces de ida y vuelta) de canciones y retahílas, así como en las imbricaciones entre poesía popular y poesía de autor.
«Bonitas y escogidas canciones»: estudio de una colección de cancioneros populares mexicanos
Authors: Gómez Mutio Ana Rosa
Published: November 2019

Este trabajo analiza los rasgos líricos de ocho cancioneros de la imprenta de Antonio Vanegas Arroyo para brindar una imagen general de los cuadernillos de canciones, de la razón de su éxito y de las características que buscaba su público. Todo ello abordado no sólo desde su contexto de producción, como desde su formato, desde su temática y a su público de dirección.
La poesía medieval como objeto de estudio en el siglo XXI: evanescencia y fijación en el medio digital
Authors: Del Rio Riande Gimena , Martínez Cantón Clara Isabel , González Blanco Elena
Published: November 2019

Las humanidades digitales como una forma de aproximación a la poesía medieval es tema que llama la atención en la actualidad, sobre todo tomando en cuenta que la poesía medieval ha pasado por difusión oral, impresa y digital. La poesía medieval en el medio digital ha ocupado un lugar privilegiado en los estudios académicos, de donde surgen nuevos elementos a estudiar: catálogos, índices, repositorios, bases de datos, etc. Además, la digitalización posibilita el uso de otras herramientas que constituyen bases de datos híbridas que conforman, a su vez, constelaciones. Ejemplo de todo ello es el proyecto POSDATA.
Enacting Citizenship and the Right to the City: Towards Inclusion through Deepening Democracy?
Authors: Hintjens Helen , Kurian Rachel
Published: November 2019

In this introductory article, the main theoretical concerns guiding this thematic issue are briefly discussed, alongside an overview of relevant literature on rights and urban citizenship. We draw on the work of Engin on ‘enacted citizenship,’ and combine Hannah Arendt’s ‘right to have rights’ with Henri Lefebvre’s ‘right to the city,’ for inspiration. The hope is that these concepts or theoretical tools help our contributors explore the ‘grey areas’ of partial inclusion and exclusion, and to connect the informal with the formal, migrants with professionals, locals with those from elsewhere. Since the contributions in this issue come from practitioners as well as scholars, we are interested in very different forms of urban citizenship being enacted in a range of settings, in such a way as to overcome, or at least side-step, social, economic and political exclusion within specific urban settings. In this introduction we reflect on urban migrants organising and mobilising to enact their own citizenship rights within specific urban spaces, and present each of the eight published articles, briefly illustrating the range of approaches and urban citizenship issues covered in this thematic issue. The examples of urban enacted citizenship practices include efforts to construct economic livelihoods, gain access to health care, promote political participation, reweave the social fabric of poor neighbourhoods, and provide sanctuary. All of which, our contributors suggest, requires the engagement of the local urban authorities to allow room for the informal, and to accept the need for improved dialogue and improved access to public services.
BANKRUPTCY REHABILITATION PROCEDURES: IMPORTANCE AND FORMS OF IMPLEMENTATION IN THE RUSSIAN AND FOREIGN LEGISLATION ON INSOLATABILITY
Authors: Zakirova M. A.
Published: November 2019

The article is devoted to the study of problems associated with the application of bankruptcy rehabilitation procedures in Russia.  The Russian and foreign legislation on insolvency is analyzed, and possible ways to improve the regulatory framework governing the use of bankruptcy rehabilitation procedures in Russian practice are
ORGANIZATION OF MULTIMODAL TRANSPORTATION UNDER UNCERTAINTY AND RISK
Authors: Krygina I. E.
Published: November 2019

The article provides a terminological interpretation of the concept of multimodal transportation and the signs that determine it. We consider the features of the transport and logistics system of our country, dictating specific requirements for logistics schemes for delivering goods. Also considered is the concept of risk, its types and factors as applied to multimodal transport. In conclusion, a unified procedure for risk management in logistics systems is formulated.
ANALYSIS OF ADMINISTRATIVE REGULATIONS FOR THE PROVISION OF PUBLIC SERVICES OF THE PENSION FUND OF THE RUSSIAN FEDERATION
Authors: Kolmakov S. V.
Published: November 2019

The quality of rendered public services depends, among other things, on the elaboration of regulatory legal acts in this area and their actual implementation by state authorities and organizations. The final regulatory legal act establishing the procedure for interaction and the standard for the provision of a specific service is the administrative regulation. This article examines the administrative regulations for the provision of public services by the Pension Fund of the Russian Federation for compliance with federal regulatory legal acts of the Russian Federation. The emphasis is on the electronic form of interaction between the state and the person.
SUSTAINABLE DEVELOPMENT AND BUSINESS PROCESS MANAGEMENT
Authors: Lisovsky A. L.
Published: November 2019

Business process management to date has not explicitly focused on sustainability as a change objective or driver. Although, approaches relating BPM and Sustainability already exist, e.g. Green BPM is the sum of all management activities that help to monitor and reduce the environmental impact of business processes in their design, improvement, implementation, or operation stages, as well as lead to cultural change within the process lifecycle. The intention behind Green BPM is the incorporation of environmental objectives into the management of business processes. To achieve this objective, BPM has to be extended by ecologically oriented complements, as are the consideration of environmental strategy as a part of the process strategy, or the awareness for energy consumption and pollution. Together with an earlier article consolidates several contributions of the BPM foundations in three underlying process change traditions: (1) the Quality Control tradition, (2) the Business Management tradition, and (3) the Information Systems (IS) tradition. These three traditions propose different approaches to business process change and each emphasizes some practices over others. Currently, there is a tendency of combining the various traditions in a comprehensive BPM approach.
Comparative analysis of concurrent and sequential chemoradiation in locally advanced lung cancer: a single institution experience
Authors: Srivastava Shelly , Saini Surendra Kumar , Agarwal S. K.
Published: November 2019

Background: Outcome of various treatment regimen are dismal in non-small cell lung cancer. This analysis is done to find possible care in authors institutional set up and to see how these protocols have effect in Indian patients in term of toxicity.Methods: Medical records and data on patients who had been diagnosed with non-small cell lung cancer histologically or cytologically, and who had been treated with sequential chemoradiation and concurrent chemoradiation at the hospital from January 2007 to March 2015 was retrospectively reviewed and analyzed. Two groups of sequential chemoradiotherapy and concurrent chemoradiotherapy were formed and compared for outcomes.Results: Of the 114 evaluable patients in sequential chemoradiotherapy group, the median survival time was 16.0 months and the 1, 3- and 5-years overall survival were 57.0, 26.9 and 21.2%, respectively. Median progression free survival (PFS was 13.0 months and the 1, 3 and 5 years PFS were 52.6, 14.6 and 7.8%, respectively. In concurrent chemoradiotherapy group (105 patients), the overall median survival time was 15 months and the 1, 3- and 5-year overall survival were 56.2, 20.6 and 14.7%, respectively. Median PFS was 13 months and the 1, 3 and 5-year PFS were 48.8, 19.7 and 10.3%, respectively. Grade 3 and 4 toxicity in both regimen groups are same and statistically not significant.Conclusions: Analysis confirm dismal outcome with standard treatment and signifies to search for care beyond conventional chemoradiotherapy.
Thyroid hormones reference ranges during each trimester of normal pregnancy subjects in Andhra Pradesh, South India
Authors: Uma V. , Babu A. V. Suresh , N. Hanumanth
Published: November 2019

Background: The Thyroid hormones normal levels are very much essential for fetal neural development and good pregnancy outcome. Thyroid insufficiency during pregnancy may be associated with adverse obstetric outcome and fetal neurodevelopmental deficits. The main objective of the study is to estimate and establish the reference ranges of thyroid hormones as it varies from place to place and inter dependent on the iodine levels as the American Thyroid Association strongly recommends to refer to population defined trimester-specific reference ranges.Methods: A longitudinal study was conducted at obstetric clinic, Kakinada city, Andhra Pradesh over a period of 18 months. The total number of normal pregnancy subjects included in this study was 126. Thyroid hormones estimation was done by Competitive electrochemiluminescence (T3 and T4) and Sandwich electrochemiluminescence (TSH) at Thyorocare Laboratory. Only 47 subjects were having all three trimester thyroid hormone levels estimated during each trimester and were followed up to full term. The 2.5th and 97.5th percentiles were calculated as the reference intervals for thyroid hormone levels during each trimester.Results: The thyroid hormone levels during first, second and third trimesters median with reference intervals - Total Triiodothyronine (T3) : 163(100.2-230), 168(121-219.2), 168(110.2-222.6) ng/dl ,Total Thyroxine (T4) : 8.4(4.05-16.64) , 10.2(4.09-18.7), 11.2(3.8-16.7) μg/dl and Thyroid Stimulating Hormone (TSH ):2.52(1.6-7.9) 3.01(1.28-7.6) 3(1.5-8.4) μIU/mlConclusions: The trimester-specific reference intervals and median for thyroid hormones during normal pregnancy have been established for pregnant South Indian women in Andhra Pradesh by full term follow-up during pregnancy using 2.5th and 97.5th percentiles and median.
Uptake of hormonal implants contraceptives compared to other forms of contraceptives in Abuja, Nigeria
Authors: Ayogu Malachy E. , Eruemulor Chibuzor C. , Olibe Augustine O.
Published: November 2019

Background: Subdermal implants are a long-term, efficacious, and easily reversible contraceptive with few adverse effects that pose no risk to the health of the users. It is increasingly accepted as a method of contraception in sub-Sahara Africa like ours. Despite this, its uptake in Nigeria has not reached optimum level. This study is aimed at evaluating the socio-demographic characteristics of subdermal implants users in Abuja, Nigeria, its acceptance rate, efficacy, and discontinuation rate.Methods: The case notes of all the women from the family planning unit were studied. Data on socio-demographic characteristics, uptake rate, side effects profile and the discontinuation rates between January 2013 and December 2017 were retrieved and analyzed using SPSS software and results expressed in numbers and percentagesResults: Out of a total of 1,716 clients that were for contraception during the period under review, 786 accepted Jadelle/Implanon accounting for 45.8% of the implants acceptance rate. Of the 786 acceptors, 485(28.3%) chose Implanon, whereas 301(17.5%) preferred Jadelle. Discontinuation rate for both implants was 20.7%; the main reasons cited by the women were desire to have more children; 51(31.35%) and irregular vaginal bleeding; 30(18.4%). The Pearl Index for the implants in the study was zero. The peak parity for implant users was para 3. Approximately 72.1% the users of all forms of contraception were Christians, while 25.4% were Muslims. About 74.0% of the users had tertiary education.Conclusions: Jadelle/Implanon uptake was relatively high compared to other forms of contraception.
A prospective study of cervical lesions diagnosed by liquid based cytology in Western Rajasthan, India population
Authors: Bagga Neha , Elhence Poonam , Rao Meenakshi , Nalwa Aasma , Khera Sudeep , Bharti Jyotsna Naresh , Singh Pratibha , Shekhar Shashank
Published: November 2019

Background: Carcinoma cervix is the second most common malignancy of women in India after breast cancer. The present study was conducted to determine the spectrum of cervical lesions by liquid-based cytology in Western Rajasthan population.Methods: It is a Prospective study on 1087 cervical samples carried over a period of 1 year. Cervical samples were taken and processed by SurePath™ LBC.Results: Of total 1087 cases 959 were negative for intraepithelial lesion or malignancy (88.22%). 88 cases (8.09%) were reported as unsatisfactory. Among the non- neoplastic cases- bacterial vaginosis was reported in 209 cases (21.8%), Candida in 77 cases (8.02%), both Candida and bacterial vaginosis in 12 cases (1.25%), reactive cellular changes in 193 cases (20.12%), and Trichomonas vaginalis in 01 case. Among pre-malignant and malignant lesions, 40 cases (4.17%) the distribution was as follows-atypical squamous cells of undetermined significance 16(1.67%), atypical squamous cell-cannot rule out high grade 08 cases (0.83%), Low grade squamous intraepithelial lesion 04 cases (0.42%), high grade squamous intraepithelial lesion 07 cases (0.73%), Atypical glandular cell favoring neoplastic 01 case (0.15%), and squamous cell carcinoma 04 cases (0.42%). Histopathological co-relation of premalignant and malignant lesions was further studied.Conclusions: Liquid based cytology is an effective screening and diagnostic procedure for cervical abnormalities. Among pre-malignant and malignant lesions, histo-pathological correlation increased with increased grade of severity of lesions. To the best of knowledge, this is the largest study of liquid based cytology in the Western Rajasthan.
A comparative evaluation of I-gel and laryngeal mask airway supreme in laparoscopic surgeries: a randomized comparative study
Authors: Verma Reetu , Kumar Nitin , . Hemlata , Verma Sateesh , Singh Dinesh
Published: November 2019

Background: Supraglottic airway device results in less hemodynamic responses during laparoscopic surgery but supraglottic airway device to be used should have higher oropharyngeal seal pressure than peak pressure for effective ventilation as laparoscopic surgery also leads to higher airway pressure. In this study the efficiency of the I-gel with SLMA is compared in patients undergoing laparoscopic cholecystectomy surgeries.Methods: Sixty patients were randomized in to two groups, group A where I-gel was considered for airway management and group B where LMA Supreme was the device chosen for airway management.Results: Oropharyngeal seal pressure was significantly lower in group A than group B, 5 minutes after insertion of airway device it was 24.90±3.03 cm H2O and 27.30±3.41 cm H2O in group A and group B, respectively and 5 minutes after creation of pneumoperitoneum it was 25.53±3.17 cm H2O and 27.57±3.36 cm H2O in group A and group B, respectively. There was significant difference in the difference between inspiratory and expiratory tidal volume between the groups at all the time periods being higher in group A than group B. Hemodynamics were comparable between the two groups. Time taken to insert the airway device and Ryle’s tube insertion was significantly lesser in group B in comparison to group A. The percentage of complications was higher in group A than group B with no significant (p>0.05) association.Conclusions: Both the I-gel and SLMA devices can be used safely in laparoscopic cholecystectomy in non-obese patients. But in SLMA group oropharyngeal seal pressure was higher with lesser leak volume in comparison to I-gel group.
Profile of urinary tract stone in Makassar, Indonesia
Authors: Sulaksono Tommy , Syahrir Syakri , Palinrungi Muhammad Asykar
Published: November 2019

Background: Urinary tract stone disease (urolithiasis) is still a significant health issue throughout the world. In Asia, regions with very high urolithiasis incidence stretch from Sudan, Saudi Arabia, United Arab Emirates, Pakistan, India, Myanmar, Thailand, Indonesia, and Philippines. Demographic and regional variations in cases of urolithiasis may provide clues to their etiology and prevalence. The aim of this study was to determine the characteristics of urolithiasis patients and their management in Makassar.Methods: This was a single centre retrospective descriptive study using data from patient medical records at Dr. Wahidin Sudirohusodo Hospital Makassar in 2015-2017. Patients’ demographic, clinical, and management characteristics were recorded.Results: From 1,166 urolithiasis patients, author found men were more dominant than women with ratio of 2.2: 1. Age distribution were more common in the range of 40-60 years (58.32%). The distribution of patients with high Body Mass Index (BMI) were relatively common included overweight 20.5% and obese 13.81%. The majority of cases were unilateral urolithiasis (89.28%), with stones were largely found in kidney (59.41%). Extracorporeal Shock Wave Lithotripsy (ESWL) was the method more commonly performed (48.13%) followed by lithotripsy.Conclusions: This study showed that urolithiasis were commonly found in the age group of 40-60 years, predominantly male, and high BMI were relatively common. The majority of cases were unilateral urolithiasis, mostly located in kidney. Urolithiasis were mostly managed by ESWL and lithotripsy.
Fusarium solani infection after antimicrobial treatment of a severe bacterial peritonitis: a case report and review of the literature
Authors: Intra Jari , Sarto Cecilia , Auricchio Sara , Pozzi Marco , Pieruzzi Federico , Brambilla Paolo
Published: November 2019

Fungal peritonitis is a rare but serious complication of peritoneal dialysis. This infection has been reported to be mostly caused by Candida species, and less frequently by a variety of other yeasts and moulds, such as Aspergillus, Penicillium, and  Fusarium spp. are commonly isolated from soil, plants and environmental surfaces, and rarely from non-immunosuppressed subjects. In this report, author describe a case of infection caused by Fusarium solani in a 59-year-old man undergoing continuous ambulatory peritoneal dialysis. The fungus was recovered from cultures of peritoneal dialysate and the pathogen identification was carried out by mass spectrometry. The patient's outcome was favorable without complications after liposomal amphotericin B treatment along with peritoneal dialysis catheter removal.
Factors and characteristics of physical violence among healthcare workers of a tertiary hospital in Enugu, Nigeria
Authors: Onyia Sussan U. Arinze , Chinawa Awoere T. , Ndu Anne C. , Okwor Tochi J. , Agwu-Umahi Olanike I. , Obionu Ifeoma , Kassy Wilson C. , Aguwa Emmanuel N. , Okeke Theodora A.
Published: November 2019

Background: Healthcare workers (HCWs) especially those involved in patients’ care are at high risk of violence. The study is aimed at determining factors and characteristics of physical violence among HCWs in a tertiary hospital in Nigeria.Methods: The descriptive cross-sectional study was carried out among 412 HCWs from March to July, 2018. Data entry and analysis were done using Epi InfoTM 3.5.4 and Chi square and Fischer’s exact test where applicable were used to ascertain factors associated with physical workplace violence.Results: Respondents included 111 (26.9%) doctors and 301 (73.1%) nurses. Prevalence of physical violence was 12.6%. Of those who experienced workplace violence, the perpetrators were mainly patients’ relatives 37 (71.2%) and patients 8 (15.4%). In majority of cases, 28 (53.8%), no action was taken against the assailant. Prevalence of physical violence was significantly higher among nurses (15.3%) than doctors (5.4%) p=0.007. It is also higher among females (14.2%) than males (7.8%), although not significantly. Furthermore, HCWs who work in newborn units were most likely to experience physical violence.Conclusions: The study revealed that physical violence is prevalent among HCWs and violence prevention programs should be instituted to address it.
Knowledge and attitude regarding HIV/AIDS among patients attending medicine outpatient department in C. C. M. Medical College and Hospital, Durg
Authors: Gupta Suresh C. , Jain Karan J. , Tiwari Anjana , Padmey Indu , Bankar Nandkishor J. , Alam Md. Naushad
Published: November 2019

Background: Acquired immunodeficiency syndrome (AIDS) is one of the deadliest diseases that modern medicine has ever had to tackle. Understanding the knowledge about HIV/AIDS in general populations will help in formulating strategy for prevention and treatment. With this background, this study was conducted to assess the knowledge and awareness about HIV/AIDS among general population.Methods: A cross sectional study was conducted at C.C.M. Medical College and Hospital, Durg during December 2018 to January 2019. The data was collected from 450 patients attending the medicine outpatient department. Study subjects were selected using convenient sampling technique. Patients giving consent and willing to participate were included in the study. The data was collected in predesigned and pretested questionnaire.Results: 77.11% study participants were aware that unprotected sex can lead to transmission of HIV, 73.78% knew that shaking hands will not transmit the disease. 79.8% knew that use of condom can prevent transmission of HIV/AIDS. Overall males had better knowledge than females. 76.85% said they would behave friendly with people having HIV/AIDS and 69.78% were willing to take care of relatives with HIV.Conclusions: This study indicates that majority participants have good knowledge about difference between HIV and AIDS, modes of transmission and prevention though they were not completely aware of it. The proportion of those with lack of correct knowledge though comparatively less, needs to be taken as a challenge and addressed through well planned health educational activities at various platforms including teaching hospital premises.
Prevalence of health risk behaviours among the school going adolescents of Gwalior township
Authors: Swami Piyush Datt , Sukla Pradeep , Kumar Sanjeev
Published: November 2019

Background: The high-risk behaviors are defined as behaviors which threat health and well-being of adolescences, youth and other society’s members. Habits and behaviour that acquired in adolescent have influence in adulthood as well. Health risk behaviors such as alcohol use, smoking, substance use, unsafe driving, unintentional injuries and violence and sexual risk behaviors among adolescents is widely prevalent. Adolescent have tendency to experiments new things, they want to explore new things because of this they have risk taking behavior, this nature is also due to hormonal surge and emotional predominance. The aim and objectives of this study was to estimate the prevalence of health risk behaviors and on the basis of key findings suggest recommendations.Methods: This study is based on self-reported experiences of health risk behavior by school going adolescents. Adolescents are selected by the simple random sampling, and data is collected by the semi-structured, self-administered questionnaire.Results: 26% adolescents experience road traffic accident, 4.5% experience assault. 11% were used to smoke, 9% nonsmoking products, 2.67% uses alcohol some time in their life. 9% adolescents experience symptoms of RTI.Conclusions: Adolescent stage is a period of various changes so they need proper care, support and counseling to cope with this transition phase.
Assessment of infrastructure of first referral unit facilities in Surguja division: a responsibility of providing emergency obstetric care
Authors: Ekka Abha , Gupta Shubra A. , Sahu Divya , Minj Anmol Madhur , Soni G. P.
Published: November 2019

Background: Availability of emergency obstetric care (EmOC) is one of interventions to reduce maternal and newborn deaths. The health system fails when effective and affordable health interventions do not reach the population, when Poor infrastructure, drugs and equipment are lacking, and qualified human resources are scarce. The objective of the present study was to assess the availability of EmOC infrastructure in first referral units (FRUs) of Surguja division, Chhattisgarh.Methods: A cross sectional study was designated with 13 FRUs of Surguja division. A semi structured, closed-ended questionnaires was observed on the basis of check list, reviewing record, and interview by available staff.Results: FRUs of Surguja division for physical infrastructure scored 68.5%, for essential medicine 69.2%, for equipments 50.7%, for instruments 45.3% and for availability of blood 34.6%, for health man power category of specialist score was 25.7% but for supportive staff 65.4%.Conclusions: Our study revealed three existing bottlenecks in the healthcare delivery system as inadequate civil infrastructure, short fall of specialists as well as inadequate supplies of drugs and equipment, hampering the function of facility.
Socio-demographic characteristics of tuberculosis patients registered under RNTCP in urban area of Jodhpur, Rajasthan
Authors: Fatehpuria Chandan M. , Bhagora Ankit , Bairwa Yogprakash , Bairwa Mahesh C.
Published: November 2019

Background: Tuberculosis affects all socio-economic persons and has an enormous economic impact on many countries. India accounts for a quarter of the global burden of tuberculosis. It is a chronic stigmatized public health and social challenge in almost all communities of the developing countries. The aim of the present study was to assess the socio-demographic characteristics of tuberculosis patients in urban area and their relation with current tuberculosis epidemiological situation.Methods: A retrospective type of study was conducted from July, 2010 to September, 2010 in tuberculosis units and data was collected from tuberculosis registers and all patients were interviewed with their consent. All collected data were transferred in a computer and analyzed using Excel worksheet. Tuberculosis patients were not on directly observed treatment, short-course (DOTS) regime and not gave consent, excluded for the study.Results: In our study, total of 363 tuberculosis patients interviewed in which maximum number i.e., 90 (24.79%) of the study population belonged to the age group 26-35 years, 216 (59.51%) were male, 195 (53.72%) were from urban population, 269 (74.1%) Hindu by religion, 278 (76.58%) married, 139 (38.29%) illiterate, 126 (34.71%) unemployed, 122 (33.61%) belonged to socioeconomic class V, 202 (55.65%) were from nuclear families. 262 (72.18%) patients had pulmonary tuberculosis and 101 (27.82%) had extra-pulmonary tuberculosis Majority of patients 264 (67.77%) were in category-I followed by 81 (22.7%) in category II. And 36 (9.92%) patients were being treated as category-III.Conclusions: It has a dreadful impact on the economic wellbeing of individual, their families and the entire community.
Assessment of knowledge, attitude and practice about menstruation and menstrual hygiene among adolescent girls in rural area of district Kathua, Jammu and Kashmir
Authors: Sangra Sonika , Choudhary Neha , Kouser Wahida , Fazal Ishrat
Published: November 2019

Background: Good hygiene practices are very much needed during menstrual period. Adolescent girls especially in rural areas often lack information about good menstrual hygienic practices. Cultural beliefs about menstruation like food taboos, not going to the religious places also have a negative impact on the adolescent girls in the rural areas. This study was undertaken with an aim to evaluate the knowledge, attitude and practices of adolescent girls regarding menstruation and menstrual hygiene.Methods: This cross- sectional study was conducted among adolescent girls from 8th to 12th class, from 5 schools of zone Budhi; which is a field practice area of department of community medicine, GMC Kathua; by simple random sampling technique. Out of total 323 girls, 300 girls willing to participate in the study (convenience sampling) were included. A semi- structured self- administered questionnaire was used.Results: A total of 300 adolescent girls were enrolled in the study. Majority of adolescent girls had fair to good knowledge and practice scores regarding menstruation, menstrual hygiene and its management but poor attitude scores due to some cultural taboos like restrictions in kitchen, religious places etc.Conclusions: Proper IEC activities need to be performed at school level and Anganwadi level to create awareness among adolescent girls regarding menstrual hygiene management so that they are able to make appropriate choices so that it enable them to lead a healthy life and prevent the girls from reproductive tract infections.
Magnitude and pattern of birth defect babies at Bhopal district early intervention center under RBSK’s 4Ds approach: an observational study
Authors: Bhatia Padma , Sawlani Harshima , Shinde Mohan , Tarachandani Rajesh
Published: November 2019

Background: As per World Health Organization (WHO) congenital anomalies are also known as birth defects, congenital disorders or congenital malformations. Most common and severe congenital anomalies are congenital heart diseases, neural tube defect and Down’s syndrome. No data on the magnitude of congenital anomalies is available in India. Thus, the study aims to determine the magnitude and pattern of babies with birth defect reporting at District Early Intervention Center, Bhopal in children aged 0 to 6 years.Methods: This was an observational study conducted at DEIC, Bhopal under Rashtriya Bal Swasthya Karyakram services, over a period of one year. Children between 0 to 6 years visiting the center and belonging to Bhopal district were included in the study. Data entry was by Microsoft Excel and analysis was by Epi info.Results: 2891 children were registered for the study out of affected children 18.7% of them were diagnosed with Developmental delays while birth defect was observed in 4.3% cases. Of the birth defect cases majority i.e. 37.7% had congenital heart disease and 16.7% had congenital hearing loss. No case of retinopathy of prematurity was registered.Conclusions: The study also helps to know the magnitude and pattern of congenital anomalies. Any effective health intervention will reduce both direct costs and out-of-pocket expenditure.
Impact of educational intervention on the tuberculosis knowledge among the medical students, Chennai
Authors: Manoharan Aravind , Chellaiyan Vinoth Gnana , M. Jasmine , Liaquathali Fasna
Published: November 2019

Background: Knowledge of tuberculosis has recognized as important tool to prevent the occupational risk of contracting tuberculosis among the medical students. Though tuberculosis health education has been recognized in the medical college’s curriculum, the studies suggest the lack of knowledge of this killer disease among the medical students. This study was conducted with objective to assess level of knowledge of tuberculosis among the medical students and impact of education intervention on their knowledge.Methods: A quasi experimental study was conducted in a tertiary care teaching hospital, Chennai from January to March 2019 with a sample size of 119. The participants were assessed using a pre-tested, semi- structured questionnaire. An educational intervention was given through audio visual aid and post-test was conducted after one month. The median scores were calculated and Wilcoxon signed ranks test was applied.Results: Among the study participants, the mean age was 20.32±0.7 years. There was a significant improvement in the adequate knowledge of etiology, risk factors, clinical features and treatment following health education intervention. The post-test median score was statistically significant in all the domains of knowledge.Conclusions: The study showed poor existing knowledge of tuberculosis among the medical students which improved with an educational intervention which implies a need to revamp the undergraduate curriculum to improve the knowledge and practices among medical students.
A cross sectional study of thrombocytopenia in malaria positive cases in a tertiary care hospital of Bareilly
Authors: Agarwal Ajay K. , Katiyar Ghanshyam D. , Khan Swati , Chaudhary Bharat C. , Sharma Mahendra , Kumar Dharmendra
Published: November 2019

Background: To find out correlation of thrombocytopenia with malaria. Malaria is a protozoal disease caused by infection with parasite of genus Plasmodium. Thrombocytopenia is a common and early sign of malarial infection and 60-80% thrombocytopenia is observed in malarial cases and present more frequently and severe in complicated P. falciparum malaria.Methods: A cross sectional study done in Central Pathological Lab of Department of Pathology, RMCH, Bareilly. Blood samples collected in ethylenediaminetetraacetic acid vial and blood smear was examined for malaria parasite within red blood cells. Malaria rapid test was done for detection of Plasmodium species and platelet count was done.Results: 780 cases of malaria was studied from September 2018 to December 2018, male predominance of 54.5%, maximum malarial positive cases 26.92% in the age group of 21-30 years, maximum 86.28% cases were of P. vivax, and thrombocytopenia was observed in 91.54% cases.Conclusions: Mostly developing countries with limited resources and trained health manpower are malaria-endemic region of world. Thrombocytopenia is associated with both P. vivax and P. falciparum infections. In our study significance association between malaria and thrombocytopenia has been observed. We suggest malaria should be a consideration in all patients with fever and thrombocytopenia.
A retrospective study comparing post-aural versus endaural approach for myringoplasty in cases of chronic otitis media
Authors: Nayyar Supreet Singh , Kaur Pawandeep
Published: November 2019

Background: Patients diagnosed with chronic otitis media mucosal disease with a mild degree of conductive hearing loss require myringoplasty as their treatment. Various approaches to myringoplasty are defined. The aim of the present study was to compare outcomes of the post-aural versus end aural approach for myringoplasty.

Methods: A retrospective cohort study of 26 patients operated over a period of one year at tertiary otorhinolaryngology center.  

Results: Distribution of approaches among post-aural and end aural was 11 and 15 patients respectively. Otorrhea was the presenting complaint in 65% (n=17) of patients. Preoperative mean pure-tone average (PTA) of all patients was 34.8 dBHL (range 28 to 40 dBHL) while postoperatively 3 months mean PTA was 21.63 and 25.13 dBHL for patients undergoing end aural and post-aural approaches respectively (p=0.008). The success rate in terms of no re-perforation was 76.9% overall, 81.81% for the end aural approach and 73.33% for the post-aural approach with no statistically significant difference (p=0.612). Disease-free survival, as calculated with Kaplan-Meier analysis, was 9.7 and 13.9 months respectively (p=0.807). Cosmetic outcome was analyzed using the scar cosmesis assessment and rating (SCAR) scale. Mean SCAR scale score in our series was 5.36 and 6.20 for patients with end aural and post-aural approaches respectively with no statistically significant difference.

Conclusions: Both approaches, end aural and post-aural, are good approaches for the purpose of myringoplasty with no statistically significant difference between the two in terms of re-perforation rates or cosmetic outcomes. However, based on our study, the end aural approach has better hearing outcomes in terms of hearing improvement.

 
Neural network technology to search for targets in remote sensing images of the Earth
Authors:   Aylamazyan Program Systems Institute of Russian Academy of Sciences, Peter the First Street, 4 “a”, Veskovo Village, Yaroslavl Region, Russia, Abramov Nikolay , Talalaev Alexander ,   Aylamazyan Program Systems Institute of Russian Academy of Sciences, Peter the First Street, 4 “a”, Veskovo Village, Yaroslavl Region, Russia, Fralenko Vitaly ,   Aylamazyan Program Systems Institute of Russian Academy of Sciences, Peter the First Street, 4 “a”, Veskovo Village, Yaroslavl Region, Russia, Shishkin Oleg ,   Aylamazyan Program Systems Institute of Russian Academy of Sciences, Peter the First Street, 4 “a”, Veskovo Village, Yaroslavl Region, Russia, Khachumov Vyacheslav ,   Aylamazyan Program Systems Institute of Russian Academy of Sciences, Peter the First Street, 4 “a”, Veskovo Village, Yaroslavl Region, Russia,   The Peoples' Friendship University of Russia, Miklukho-Maklaya Street, 6, Moscow, Russia
Published: November 2019

The paper introduces how multi-class and single-class problems of searching and classifying target objects in remote sensing images of the Earth are solved. To improve the recognition efficiency, the preparation tools for training samples, optimal configuration and use of deep learning neural networks using high-performance computing technologies have been developed. Two types of CNN were used to process ERS images: a convolutional neural network from the nnForge library and a network of the Darknet type. A comparative analysis of the results is obtained. The research showed that the capabilities of convolutional neural networks allow solving simultaneously the problems of searching (localizing) and recognizing objects in ERS images with high accuracy and completeness.
Identification of thawed and frozen soil state in some Siberia regions by multi-temporal Sentinel 1 radar data in 2017-2018
Authors: Rodionova N V
Published: November 2019

The paper deals with the identification of thawed/frozen soils in the topsoil layer for
three stations in Siberia: Salekhard, Tiksi and Norilsk by using Sentinel 1B C-band radar data for the period of 2017-2018. Determination of the frozen/thawed soil state is carried out in three ways: 1) by multi-temporal radar data on the basis of a significant in 3-5 dB difference in the backscatter coefficient 0 in the transition of freezing/thawing soil state, 2) by finding the threshold value of 0  at which the temperature in the topsoil layer falls below 00C, 3) by texture features. The first method allows determining the period of time during which the process of freezing/thawing of the soil occurs. The second and third methods allow making local maps of frozen/thawed soils. It is shown that for the studied areas the Spearman correlation coefficient between 0  and air temperature for cross - polarization exceeds the correlation coefficient for co-polarization. The graphs of the AFI (air freezing index) for the period of 2012-2018 are constructed based on the archive data of air temperature for the study areas.
An investigation into the classroom talk of Iranian EFL novice vs. experienced teachers
Authors: Ghajarieh Amir , Jalali Nastaran , Mozaheb Mohammad Amin
Published: November 2019

This study investigates the classroom talk of Iranian EFL novice versus experienced teachers with emphasis on the quality of communicative features through a linguistic lens provided by the SETT (Self-Evaluation of Teacher Talk) framework and TTFS (Teacher Talk Functional Scale) checklist. In so doing, 10 intermediate-level classrooms running by five novice and five experienced teachers were observed, each case twice. Eight distinctive communicative features of TT emerged upon the initial analysis of database obtained from the audio-recordings of 20 class sessions, totaling 30 hours of naturally generated input. Subsequently, the audio-recorded materials were carefully transcribed and analyzed in correspondence with the observation data in an attempt to compare how novice and experienced teachers present their talk. The results indicated both novice and experienced teachers enact communicative aspects of classroom talk; however, the quality of presentation in the case of the experienced group was far better. This in turn highlights the importance of raising awareness regarding TT features in teacher training courses. New communicative aspects of teacher talk highlighted in this study, including the use of L1 and language gradation, would help define new research paths exploring the classroom discourse. Further research inspired by this study needs to explore other aspects of teacher-student interactions in various educational settings. Keywords: classroom talk, teacher talk, discourse, novice teachers, teacher education
Quantitative analysis of the level of p53 and p21(WAF1) mRNA in human colon cancer HT-29 cells treated with inositol hexaphosphate.
Authors: Weglarz Ludmiła , Molin Izabela , Orchel Arkadiusz , Parfiniewicz Beata , Dzierzewicz Zofia
Published: November 2019

The aim of this study was to analyze the molecular mechanism of inositol hexaphosphate (InsP(6)) action through which it may inhibit proliferation of colon cancer cells and cell cycle progression. A kinetic study of p53 and p21(WAF1) mRNA increase was performed on human colon cancer HT-29 cells after treatment with 1, 5 and 10 mM InsP(6) for 6, 12, 24 and 48 h. Real-time-QPCR based on TaqMan methodology was applied to analyze quantitatively the transcript levels of these genes. The transcription of beta-actin and GAPDH genes was assessed in parallel to select the control gene with least variability. The 2(-Delta Delta Ct) method was used to analyze the relative changes in gene transcription. InsP(6) stimulated p53 and p21(WAF1) expression at the mRNA level, with the highest increase in p21(WAF1) mRNA occurring at 24 h, i.e., following the highest increase in p53 mRNA observed at 12 h. Based on these studies it may be concluded that the ability of InsP(6) to arrest the cell cycle may be mediated by the transcriptional up-regulation of the p53-responsive p21(WAF1) gene.
Recombinant VP60 in the form of virion-like particles as a potential vaccine against rabbit hemorrhagic disease virus.
Authors: Gromadzka Beata , Szewczyk Bogusław , Konopa Grazyna , Fitzner Andrzej , Kesy Andrzej
Published: November 2019

Rabbit hemorrhagic disease virus (RHDV) which causes a highly contagious disease of wild and domestic rabbits belongs to the family Caliciviridae. It is a small, positive single-stranded RNA virus with a genome of 7.5 kb and has a diameter of approximately 40 nm. In negatively stained electron micrographs the virus shows typical calicivirus morphology with regularly arranged cup-shaped structures on the surface. It is a major pathogen of rabbits in many countries. Vp60 - a coat protein of molecular mass around 60 kDa is the major antigen of RHDV. It is present as 90 dimeric units per virion particle. We have expressed VP60 gene in the baculovirus system with the aim to use it as a potential vaccine against RHDV and a diagnostic reagent in immunological tests. cDNA of the vp60 gene of strain SGM, was cloned into a baculovirus transfer vector as full-length gene, as well as truncated gene lacking 600 5'-terminal nucleotides. The sequence of SGM VP60 differed markedly from that of the reference strain. Full-length recombinant VP60 protein from the SGM strain self-assembled to form virus-like particles (VLPs). These particles observed by electron microscopy were morphologically similar to native virions and were able to agglutinate human group 0 erythrocytes. After immunization the recombinant particles induced RHDV-specific antibodies in rabbits and guinea pigs. Rabbits immunized with the VLPs were fully protected against challenge with a virulent RHDV.