bluuebunny/crossref_metadata_2025 huggingface dataset:
Increasing CB2 Receptor Activity after Early Life Stress Prevents Depressive Behavior in Female Rats
Authors: Andersen Susan L.
Published: April 2024

Early adversity, the loss of the inhibitory GABAergic interneuron parvalbumin, and elevated neuroinflammation are associated with depression. Individuals with a maltreatment history initiate medicinal cannabis use earlier in life than non-maltreated individuals, suggesting self-medication. Female rats underwent maternal separation (MS) between 2 and 20 days of age to model early adversity or served as colony controls. The prelimbic cortex and behavior were examined to determine whether MS alters the cannabinoid receptor 2 (CB2), which has anti-inflammatory properties. A reduction in the CB2-associated regulatory enzyme MARCH7 leading to increased NLRP3 was observed with Western immunoblots in MS females. Immunohistochemistry with stereology quantified numbers of parvalbumin-immunoreactive cells and CB2 at 25, 40, and 100 days of age, revealing that the CB2 receptor associated with PV neurons initially increases at P25 and subsequently decreases by P40 in MS animals, with no change in controls. Confocal and triple-label microscopy suggest colocalization of these CB2 receptors to microglia wrapped around the parvalbumin neuron. Depressive-like behavior in MS animals was elevated at P40 and reduced with the CB2 agonist HU-308 or a CB2-overexpressing lentivirus microinjected into the prelimbic cortex. These results suggest that increasing CB2 expression by P40 in the prelimbic cortex prevents depressive behavior in MS female rats.
Correction: D’Amico et al. Toxic Exposure to Endocrine Disruptors Worsens Parkinson’s Disease Progression through NRF2/HO-1 Alteration. Biomedicines 2022, 10, 1073
Authors: D’Amico Ramona , Gugliandolo Enrico , Siracusa Rosalba , Cordaro Marika , Genovese Tiziana , Peritore Alessio Filippo , Crupi Rosalia , Interdonato Livia , Di Paola Davide , Cuzzocrea Salvatore , Fusco Roberta , Impellizzeri Daniela , Di Paola Rosanna
Published: April 2024

Errors in Figures [...]
The Significance of Selected Myokines in Predicting the Length of Rehabilitation of Patients after COVID-19 Infection
Authors: Mińko Alicja , Turoń-Skrzypińska Agnieszka , Rył Aleksandra , Mańkowska Katarzyna , Cymbaluk-Płoska Aneta , Rotter Iwona
Published: April 2024

In the context of the global COVID-19 pandemic, understanding the intricate mechanisms of the body’s response to infection and inflammation has become a priority for the medical and research communities. It has been proven that during COVID-19 infection, molecules are secreted—namely organokines, which may directly or indirectly play a role in the pathophysiology of COVID-19. The objective of this study was to scrutinize the potential correlation between the levels of selected myokines (myostatin, agrin, irisin, and myonectin) and the duration of rehabilitation in post-COVID-19 patients. Additionally, the study aimed to investigate whether there is a correlation between the levels of these myokines and the length of hospitalization during COVID-19 treatment. The study was conducted at the Rehabilitation Hospital in Szczecin (Poland). Patients in the study participated in a comprehensive rehabilitation program following COVID-19 treatment. In order to assess the effectiveness of rehabilitation, the following tests were performed: a 6 min walk test with an assessment of exercise tolerance (Borg scale), an assessment of dyspnea severity (mMRC scale), a spirometric assessment of respiratory function, a measurement of arm strength, and an assessment of fatigue using the Fatigue Assessment Scale (FAS). Myokine levels were measured using commercially available enzyme-linked immunosorbent assays (ELISA) according to the manufacturer’s instructions. Statistical analysis was performed using Statistica 13.1 software. Lower concentrations of irisin and myonectin and higher concentrations of myostatin correlated with longer rehabilitation time. Baseline levels of specific myokines in post-COVID-19 patients could play a crucial role in anticipating the duration of rehabilitation. The duration of hospitalization for the infection may influence myokine levels in patients recovering from COVID-19.
Radiological Imaging Findings of Adrenal Abnormalities in TAFRO Syndrome: A Systematic Review
Authors: Kurokawa Ryo , Baba Akira , Kano Rui , Kaneko Yo , Kurokawa Mariko , Gonoi Wataru , Abe Osamu
Published: April 2024

This systematic review article aims to investigate the clinical and radiological imaging characteristics of adrenal abnormalities in patients with thrombocytopenia, anasarca, fever, reticulin fibrosis, renal dysfunction, and organomegaly (TAFRO) syndrome. We searched the literature in PubMed, the Cochrane Library, and the Web of Science Core Collection. Ultimately, we analyzed 11 studies with 22 patients plus our 1 patient, totaling 23 patients. The mean age was 47.0 ± 12.6 years. There were 20 male and 3 female patients, respectively. The histopathological analysis of lymph nodes was conducted in 15 patients (65.2%), and the diagnosis was consistent with TAFRO syndrome in all 15 patients. Among the 23 patients, 11 patients (18 adrenal glands) showed adrenal ischemia/infarction, 9 patients (13 adrenal glands) showed adrenal hemorrhage, and 4 patients (7 adrenal glands) showed adrenomegaly without evidence of concurrent ischemia/infarction or hemorrhage. One patient demonstrated unilateral adrenal hemorrhage and contralateral adrenomegaly. In patients with adrenal ischemia/infarction, the adrenal glands displayed poor enhancement through contrast-enhanced computed tomography (CT). In patients with adrenal hemorrhage, the adrenal glands revealed high attenuation through non-enhanced CT and hematoma through magnetic resonance imaging. Adrenomegaly, with or without adrenal ischemia/infarction or hemorrhage, was observed in all patients (23/23, 100%). The subsequent calcification of the affected adrenal glands was frequently observed (9/14, 64.3%) when a follow-up CT was performed. Abdominal pain was frequent (15/23, 65.2%), all of which occurred after the disease’s onset, suggesting the importance of considering TAFRO syndrome as a cause of acute abdomen. Given the absence of evidence of adrenal abnormalities in non-TAFRO-idiopathic multicentric Castleman disease (iMCD), they may serve as diagnostic clues for differentiating TAFRO syndrome from non-TAFRO-iMCD.
Beyond Sleep: Investigating User Needs in Today’s Bedrooms
Authors: Dincer Demet , Tietz Christian , Dalci Kerem
Published: April 2024

This study investigates the current use of bedrooms to fill a knowledge gap in the existing sleep studies literature by focusing on user preferences and their needs. Despite substantial research into the optimal physical conditions for sleep, there remains a gap in understanding how individuals use their bedrooms today. As an initial step to bridge this gap, we employed a mixed-method research approach, integrating quantitative and qualitative data results. A survey including multiple-choice and open-ended questionnaire items was undertaken in Australia with 304 participants. Our research findings indicate that ‘having a separate bedroom in a house’ is the most common arrangement. However, other arrangements, such as living in a bedsit or sharing a bedroom, are indicators of diverse bedroom conditions. In total, 70% of respondents stated that they would like to make changes to their bedrooms for physical, functional, comfort-based, and aesthetic reasons, with comfort ranked as the highest. The majority of respondents stating they would not make any modifications in their bedrooms were found to be owner-occupiers. Mattress quality and privacy emerged as the most significant factors impacting the respondents’ bedroom experience, and female participants were reported to place higher importance on cleanliness compared to male participants. Additionally, preferences for sleeping alone or with a partner were found to shift with age. Based on our findings, we suggest that survey-based sleep studies should evaluate the bedroom arrangements before the individual’s sleep habits. Further studies are needed to understand how privacy needs influence sleep quality. From a built environment viewpoint, this study emphasizes the need to develop bedroom design solutions tailored to optimize bedroom conditions, particularly for rental tenants who often have limited control over the physical conditions of their sleep environment.
A Retrospective Review and Comprehensive Tumour Profiling of Advanced Non-Melanomatous Cutaneous Spindle Cell Neoplasms Treated with Immune-Checkpoint Inhibitors
Authors: McLean Luke S. , Lim Annette M. , Angel Christopher , Young Richard J. , Pizzolla Angela , Archer Stuart , Solomon Benjamin J. , Thai Alesha A. , Lewin Jeremy , Rischin Danny
Published: April 2024

Non-melanomatous cutaneous spindle cell neoplasms are a rare group of malignancies that present a diagnostic challenge, and for which there is a lack of consensus on how to best manage patients with advanced disease and only limited reports of immune-checkpoint inhibitor (ICI) responses. In this study, we performed a single-center retrospective review of treatment outcomes for all advanced non-melanomatous cutaneous spindle cell neoplasms treated with ICIs. Blinded histopathology reviews occurred to confirm each diagnosis. Comprehensive tumour profiling included whole exome sequencing for tumour mutational burden (TMB) and ultraviolet(UV) signatures, and immunohistochemistry for immune-cell infiltration (CD4/CD3/CD8/CD103/CD20) and immune-checkpoint expression (PD-L1/LAG3/TIGIT). Seven patients were identified. The objective response rate was 86% (6/7) with five complete responses (CR). Responses were durable with two patients in CR > 30 months after ICI commencement. All patients had high TMB and UV signatures. One patient had PD-L1 100% (combined positive score) with abundant immune-cell infiltration and LAG3 expression. In advanced non-melanomatous cutaneous spindle cell neoplasms, excellent responses to ICIs with durable disease control were observed. ICIs are worthy of further exploration in these patients. UV signatures and high TMB could be used to help select patients for treatment.
Emerging and Clinically Accepted Biomarkers for Hepatocellular Carcinoma
Authors: Fares Sami , Wehrle Chase J. , Hong Hanna , Sun Keyue , Jiao Chunbao , Zhang Mingyi , Gross Abby , Allkushi Erlind , Uysal Melis , Kamath Suneel , Ma Wen Wee , Modaresi Esfeh Jamak , Linganna Maureen Whitsett , Khalil Mazhar , Pita Alejandro , Kim Jaekeun , Walsh R. Matthew , Miller Charles , Hashimoto Koji , Schlegel Andrea , Kwon David Choon Hyuck , Aucejo Federico
Published: April 2024

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death and the sixth most diagnosed malignancy worldwide. Serum alpha-fetoprotein (AFP) is the traditional, ubiquitous biomarker for HCC. However, there has been an increasing call for the use of multiple biomarkers to optimize care for these patients. AFP, AFP-L3, and prothrombin induced by vitamin K absence II (DCP) have described clinical utility for HCC, but unfortunately, they also have well established and significant limitations. Circulating tumor DNA (ctDNA), genomic glycosylation, and even totally non-invasive salivary metabolomics and/or micro-RNAS demonstrate great promise for early detection and long-term surveillance, but still require large-scale prospective validation to definitively validate their clinical validity. This review aims to provide an update on clinically available and emerging biomarkers for HCC, focusing on their respective clinical strengths and weaknesses.
Radiomics Machine Learning Analysis of Clear Cell Renal Cell Carcinoma for Tumour Grade Prediction Based on Intra-Tumoural Sub-Region Heterogeneity
Authors: Alhussaini Abeer J. , Steele J. Douglas , Jawli Adel , Nabi Ghulam
Published: April 2024

Background: Renal cancers are among the top ten causes of cancer-specific mortality, of which the ccRCC subtype is responsible for most cases. The grading of ccRCC is important in determining tumour aggressiveness and clinical management. Objectives: The objectives of this research were to predict the WHO/ISUP grade of ccRCC pre-operatively and characterise the heterogeneity of tumour sub-regions using radiomics and ML models, including comparison with pre-operative biopsy-determined grading in a sub-group. Methods: Data were obtained from multiple institutions across two countries, including 391 patients with pathologically proven ccRCC. For analysis, the data were separated into four cohorts. Cohorts 1 and 2 included data from the respective institutions from the two countries, cohort 3 was the combined data from both cohort 1 and 2, and cohort 4 was a subset of cohort 1, for which both the biopsy and subsequent histology from resection (partial or total nephrectomy) were available. 3D image segmentation was carried out to derive a voxel of interest (VOI) mask. Radiomics features were then extracted from the contrast-enhanced images, and the data were normalised. The Pearson correlation coefficient and the XGBoost model were used to reduce the dimensionality of the features. Thereafter, 11 ML algorithms were implemented for the purpose of predicting the ccRCC grade and characterising the heterogeneity of sub-regions in the tumours. Results: For cohort 1, the 50% tumour core and 25% tumour periphery exhibited the best performance, with an average AUC of 77.9% and 78.6%, respectively. The 50% tumour core presented the highest performance in cohorts 2 and 3, with average AUC values of 87.6% and 76.9%, respectively. With the 25% periphery, cohort 4 showed AUC values of 95.0% and 80.0% for grade prediction when using internal and external validation, respectively, while biopsy histology had an AUC of 31.0% for the classification with the final grade of resection histology as a reference standard. The CatBoost classifier was the best for each of the four cohorts with an average AUC of 80.0%, 86.5%, 77.0% and 90.3% for cohorts 1, 2, 3 and 4 respectively. Conclusions: Radiomics signatures combined with ML have the potential to predict the WHO/ISUP grade of ccRCC with superior performance, when compared to pre-operative biopsy. Moreover, tumour sub-regions contain useful information that should be analysed independently when determining the tumour grade. Therefore, it is possible to distinguish the grade of ccRCC pre-operatively to improve patient care and management.
NMR Metabolomics of Primary Ovarian Cancer Cells in Comparison to Established Cisplatin-Resistant and -Sensitive Cell Lines
Authors: Ghini Veronica , Sorbi Flavia , Fambrini Massimiliano , Magherini Francesca
Published: April 2024

Cancer cell lines are frequently used in metabolomics, such as in vitro tumor models. In particular, A2780 cells are commonly used as a model for ovarian cancer to evaluate the effects of drug treatment. Here, we compare the NMR metabolomics profiles of A2780 and cisplatin-resistant A2780 cells with those of cells derived from 10 patients with high-grade serous ovarian carcinoma (collected during primary cytoreduction before any chemotherapeutic treatment). Our analysis reveals a substantial similarity among all primary cells but significant differences between them and both A2780 and cisplatin-resistant A2780 cells. Notably, the patient-derived cells are closer to the resistant A2780 cells when considering the exo-metabolome, whereas they are essentially equidistant from A2780 and A2780-resistant cells in terms of the endo-metabolome. This behavior results from dissimilarities in the levels of several metabolites attributable to the differential modulation of underlying biochemical pathways. The patient-derived cells are those with the most pronounced glycolytic phenotype, whereas A2780-resistant cells mainly diverge from the others due to alterations in a few specific metabolites already known as markers of resistance.
A Dynamic Range Preservation Readout Integrated Circuit for Multi-Gas Sensor Array Applications
Authors: Kwon Soon-Kyu , Kim Hyeon-June
Published: April 2024

This study introduces a readout integrated circuit (ROIC) tailored for multi-gas sensor arrays featuring a proposed baseline calibration scheme aimed at mitigating the issue of sensor baseline variation. Unlike previous approaches, the proposed scheme stores each sensor’s baseline value and dynamically updates the signal extraction range accordingly during ROIC operation. This adjustment allows for the optimal use of the ROIC’s dynamic range, enhancing sensor uniformity and accuracy without the need for complex additional circuitry or advanced post-processing algorithms. We fabricated a prototype ROIC using a 180 nm CMOS process, achieving a low power consumption of 0.43 mW and a conversion rate of 50 kSPS. The prototype boasts an integrated noise level of 9.9 μVRMS across a frequency range of 0.1 Hz to 5 kHz and a dynamic range of 142.6 dB, coupled with superior linearity, indicated by a maximum integral non-linearity (INL) of −75.71 dB. This design significantly reduces sensor offset scattering to within 1 LSB of the A/D reference scale. In this study, the efficacy of the proposed scheme was validated using Figaro TGS-2600. The ROIC targets a sensitivity range from 0.54 to 0.23 for gas concentrations ranging from 5 ppm to 20 ppm and a resolution of 39 Ω for sensor resistance range from 10 kΩ to 90 kΩ. The enhancements in performance make the proposed ROIC a promising solution for precise gas concentration detection in sensor applications.
Editorial: Celebrating Diversity and Advancements in Pediatric Cardiology—A Journey through Specialized Research
Authors: Mapelli Massimo , Zagni Paola , Picciolli Irene
Published: April 2024

The field of pediatric cardiology is as vast and diverse as the young patients it serves (Figure 1) [...]
Effect of Iron Content on the Pitting Corrosion Behavior of Laser-Cladded Ni-Cr-Mo Alloy Coating in a Simulated Seawater Environment
Authors: Liu Quanbing , Liu Zongde , Shen Yue , Kong Yao , Li Jiaxuan , Zhang Yutong , Ning Huaqing
Published: April 2024

In order to study the effect of iron content on the pitting corrosion behavior of a Ni-Cr-Mo alloy coating in a simulated seawater environment, a Ni-Cr-Mo-xFe (x = 0, 5, 10, 15, 20, 25) alloy coating was prepared through laser cladding technology. These coatings primarily consist of a γ-Ni solid solution phase, with observable iron segregation in the interdendritic regions when the iron content reaches 25 wt%. After 42 days of salt spray corrosion, it was found that pitting began to appear on the surface when the iron content in the coating increased to 10 wt%. The results of electrochemical behavior revealed that the coatings with iron contents in a range of 10–25 wt% exhibited metastable pitting characteristics, and the impedance modulus decreased with the increase in iron content. Pitting corrosion occurs due to selective corrosion of the dendritic regions. When the iron content exceeds 10 wt%, the accumulation of iron in the outer layer of the passivation film would lead to an excess of cationic vacancies, and the stability of the passive film is then reduced. This study provides a reference for the control of the iron content in a Ni-Cr-Mo alloy coating when applied in marine environments.
Adsorption Layer Properties and Foam Behavior of Aqueous Solutions of Whey Protein Isolate (WPI) Modified by Vacuum Cold Plasma (VCP)
Authors: Ommat Mohammadi Elham , Yeganehzad Samira , Hesarinejad Mohammad Ali , Dabestani Mohsen , von Klitzing Regine , Miller Reinhard , Schneck Emanuel
Published: April 2024

For years, cold plasma processing has been used as a non-thermal technology in industries such as food. As interfacial properties of protein play a remarkable role in many processes, this study investigates the effect of cold plasma on the foaming and interfacial behavior of WPI. The objective of this study is to evaluate the effect of different gases (air, 1:1 argon–air mixture, and sulfur hexafluoride (SF6)) used in low-pressure cold plasma (VCP) treatments of whey protein isolate (WPI) on the surface and foaming behavior of aqueous WPI solutions. Dynamic surface dilational elasticity, surface tension isotherms, surface layer thickness, and the foamability and foam stability were investigated in this study. VCP treatment did not significantly affect the adsorption layer thickness. However, an increase in induction time, surface pressure equilibrium value, and aggregated size is observed after SF6VCP treatment, which can be attributed to the reaction of WPI with the reactive SF6 species of the cold plasma. The surface dilational elastic modulus increased after VCP treatment, which can be related to the increased mechanical strength of the protein layer via sulfonation and aggregate formation. VCP treatment of WPI increases the foam stability, while the average diameter of foam bubbles and liquid drainage in the foam depends on the gas used for the cold plasma.
Determinants of the Management of Native Vegetation on Farms
Authors: Kaine Geoff , Wright Vic
Published: April 2024

The clearing of native vegetation on private agricultural land has contributed greatly to the loss of ecosystems and biodiversity worldwide. Native vegetation on private land may be cleared for a variety of reasons, of which the expansion of agriculture is only one. In this study, we investigate how the clearing of native vegetation on private land is influenced by (1) the utilitarian, social and hedonic objectives of landholders and (2) the way in which the presence of native vegetation interacts with the farm system to contribute to, or detract from, achieving those objectives. Using data from a survey of agricultural landholders in New South Wales, Australia, we found that the landholders’ management of native vegetation was strongly influenced by their perceptions of the opportunities and threats the native vegetation on their properties presented to them. The implications are drawn for predicting the clearing of native vegetation and designing effective policy interventions to influence the extent of clearing.
Fatigue Analysis of the Nickel-Based Superalloy Inconel 617 by Fatigue Experiments and EBSD Data-Based Finite Element Simulations in Correlation with E·m Theories
Authors: Liesegang Moritz , Beck Tilmann
Published: April 2024

Nickel-based superalloys exhibit pronounced elastic anisotropy and, hence, the local grain orientation strongly affects the stress and strain distribution in the material under mechanical loadings. Therefore, the crack initiation and failure behaviour of components made from nickel-based superalloys are complex and hardly predictable. A better fundamental understanding of the phenomena that occur in nickel-based superalloys under a quasistatic and cyclic load is therefore desired. Previously, a continuum mechanics-based model has been successfully developed, considering the grain structure, the elastic anisotropy, and the Schmid factor, based on data from electron backscatter diffraction (EBSD). The E·m model was confirmed by the finite element method (FEM) simulations and experimental observations regarding the resulting average stresses and strains in the individual grains as well as the formation of slip bands under a quasistatic load with few restrictions. The behaviour under cyclic loadings has been investigated in this work to correlate the mechanical behaviour, simulated by the previously developed FE models, with the local stiffness and Schmid factors considering fatigue failure. For this purpose, the fatigue behaviour of Inconel 617 samples was characterised up to the high-cycle fatigue (HCF) regime, accompanied by EBSD measurements for stress amplitudes that resulted in strains close to the elastic–plastic regime. The EBSD data were used to create digital twins of the samples to simulate the mechanical reaction to a displacement similar to the associated strain of the fatigue tests. An analysis of the fractured samples by scanning electron microscopy was performed to retrace the location of the crack initiation supported by the EBSD measurements before and after fatigue testing. Two samples were investigated in detail that showed different fracture types. Sample 1 showed transcrystalline failure in a grain that showed a high Young’s modulus, Schmid factor, and resolved shear stress that indicates a failure due to the properties of the grain itself. In contrast, an intercrystalline failure was observed for sample 2 that showed large differences in the orientation and, hence, largely different mechanical properties in the area of failure as well. The observed failure types, the resulting stresses and strains calculated by the FE model, and the consideration of the E·m model showed an agreement of all the methods. Therefore, the findings of this work complement previous investigations of the mechanical behaviour of coarse-grained anisotropic nickel-based superalloys with a focus on the orientations of the grains towards the loading direction.
Exploring Morphological Population Variability: Host Plant and Habitat Dependency in the Protected Moth Gortyna borelii (Lepidoptera, Noctuidae)
Authors: Rákosy László , Martin Mihai Alexandru , Sitar Geanina Magdalena , Crișan Andrei , Sitar Cristian
Published: April 2024

In this paper, we discuss the evolutionary implications of the correlation between different species of Peucedanum plants and the distribution of Gortyna borelii moth populations in Romania. We highlight geographic separation and isolation among these populations due to anthropogenic landscape fragmentation, which hinders genetic exchange. A geometric morphometric analysis was utilized to visualize and compare the morphometric variations in relation to the environmental variables, particularly the host plant. Additionally, the distribution of G. borelii populations across Europe and in Romania that are correlated with the host plant was analyzed. The significant morphological and morphometric differences between the analyzed populations support our working hypothesis, according to which the use of different Peucedanum species by the larvae of G. borelii leads to an intraspecific diversification correlated with the host plant species. The newly discovered population of G. borelii in Romania holds substantial conservation importance, necessitating protection measures, including demarcating habitat areas and raising awareness among stakeholders. G. borelii is a protected species at the European level (Habitats Directive 92/43/EEC, Appendices II and IV), considered endangered due to the isolation of its populations and anthropogenic pressures exerted through agricultural practices. Understanding the impact of agricultural practices on their habitat is crucial for effective management strategies. Overall, this study sheds light on the complex interplay between ecological adaptation, host plant specialization, and speciation dynamics in phytophagous insects, emphasizing the importance of conservation efforts to preserve G. borelii populations and their habitats.
Negative Outcomes of Blepharoplasty and Thyroid Disorders: Is Compensation Always Due? A Case Report with a Literature Review
Authors: Defraia Beatrice , Focardi Martina , Grassi Simone , Chiavacci Giulia , Faccioli Simone , Romano Gianmaria Federico , Bianchi Ilenia , Pinchi Vilma , Innocenti Alessandro
Published: April 2024

Background: Plastic surgery is one of the medical specialties with the highest risk of recurrent medical malpractice claims. The frequency of civil lawsuits represents an issue for the micro- and macro-economy of practitioners of these health treatments. This paper aims to discuss the medico-legal aspects and claim path in a case of a cosmetic blepharoplasty complicated by lagophthalmos wrongly related to the procedure but due to missed hyperthyroidism. Case Description and Literature Review: A 48-year-old woman who underwent cosmetic blepharoplasty with undiagnosed hyperthyroidism claimed that the lagophthalmos that occurred some months after the procedure was due to medical malpractice, due to an over-resection of the exuberant lower eyelid tissue. The review question was, “Are thyroid disfunctions usually considered contraindications to be communicated to patients who undergo blepharoplasty?”, and the databases MEDLINE via PubMed, Embase, Scopus, Ovid, ISI Web of Science, Cochrane, and Google Scholar were used. Results and Discussion: There were 21 eligible papers. The case highlights the importance and complexity of causal inference (such as unknown thyroid dysfunctions), related informed consent involving information on possible complications unrelated to malpractice, and guidelines recommending endocrinological consultation for cosmetic/functional blepharoplasty in patients at risk (e.g., female patients with a known history of thyroid disease).
Effectiveness of Autologous Platelet Concentrates in the Sinus Lift Surgery: Findings from Systematic Reviews and Meta-Analyses
Authors: Gasparro Roberta , Di Lauro Alessandro Espedito , Campana Maria Domenica , Rosiello Nicola , Mariniello Mauro , Sammartino Gilberto , Marenzi Gaetano
Published: April 2024

Maxillary sinus augmentation is one of the most predictable procedures for the rehabilitation of the posterior maxilla. The current overview aimed to summarize the findings provided by systematic reviews (SRs) and meta-analyses on the effectiveness of autologous platelet concentrates (APCs) in sinus lift and to assess the methodological quality of the included SRs. Three electronic databases have been explored. SRs and meta-analyses addressing the effectiveness of APCs in sinus lift technique were included. Clinical, radiographic and histomorphometric findings were considered for APCs as solely grafting materials and APCs in combination with biomaterials. Outcomes were implant survival rate (ISR), implant stability (IS), implant failure (IF), postoperative complications, histomorphometric findings, radiographic bone gain, bone volume and bone density. The methodological quality of the included SRs was assessed using the updated version of “A Measurement Tool to Assess Systematic Review” (AMSTAR-2). Thirty SRs were included. The methodological quality of the included reviews ranged from critically low (3 studies) to high (9 studies). The included SRs showed favorable clinical outcomes, short-term new bone formation and no biological complications when APCs were used both as solely graft material or in combination with other biomaterials. However, no significant additional effects in the long-term period were observed. APCs did not add any further positive effects compared to the physiological healing derived by the natural blood clot. The current overview of SRs highlighted the need for high-quality SRs evaluating the role of APCs in sinus lift though network meta-analyses, in order to identify the most powerful material for sinus lift augmentation. The use of APCs improves the healing of soft tissues and the postoperative quality of life in the short-term period. Thus, its application can be recommended.
Effect of Probiotics Lactobacillus rhamnosus and Lactobacillus plantarum on Caries and Periodontal Diseases: A Systematic Review
Authors: D’Agostino Silvia , Valentini Giulia , Iarussi Francesca , Dolci Marco
Published: April 2024

Caries and periodontitis are the most prevalent oral diseases worldwide. Major factors contributing to the development of these oral conditions include poor oral hygiene, dental biofilm formation, high carbohydrates diet, smoking, other systemic diseases, and genetic factors. Various preventive measures have been established to mitigate the risk of caries and periodontal disease development. The present review aims to discuss the role of the probiotics Lactobacillus rhamnosus and Lactobacillus plantarum in the prevention and treatment of caries and periodontal diseases. The study was conducted in accordance with PRISMA guidelines and was registered on PROSPERO. The search involved PubMed, Web of Science, and Scopus and considered the PICO format. Studies were screened by two reviewers independently, and disagreements were solved by consensus with a third reviewer. Data extraction included details about the type of probiotics, strains, and purpose of administration. A total of 15 RCTs were included, of which just 1 was about tooth cavities. Overall, 87% of the included studies were good-quality papers regarding the Jadad Scale. Several studies agreed on the potential of probiotics L. rhamnosus and L. plantarum, both alone and combined, to prevent and improve clinical outcomes in caries and periodontal treatments, weaker evidence is provided for the microbiological benefit.
Out-of-School Exposure to English in EFL Teenage Learners: Is It Related to Academic Performance?
Authors: Tran Linh , Miralpeix Imma
Published: April 2024

Learning a Foreign Language (FL) beyond the classroom has become common practice thanks to advances in technology and the use of English as a Lingua Franca. This study explores the types and amount of out-of-school informal exposure to English that Spanish secondary school students typically receive in their daily lives. Informed by recent literature on the influence of extramural activities on FL proficiency, the second aim of this study is to investigate the potential relationship between out-of-school exposure and academic performance, as measured by English school grades. Data were obtained from a questionnaire answered by secondary school students aged 12–16 (N = 2015) regarding the different types and amounts of activities they perform in English outside school. Findings revealed that teenage learners were most frequently exposed to English through audiovisual input. Social media interaction, along with reading and writing (with or without digital support), were closely associated with their English marks. Other popular activities, such as listening to music or playing video games, were not found to be related to proficiency or even showed a negative correlation with it, while less popular activities, such as watching subtitled movies and series, could have greater potential for language learning. This study contributes to the understanding of informal practices in FL learning settings and provides insights that can help bridge interactive language practices and formal curriculum to create holistic learning experiences for language learners.
Can We Measure the Level of Socio-Emotional Competencies of Adolescents?
Authors: Portela-Pino Iago , Domínguez-Alonso Jose , Alvariñas-Villaverde Myriam
Published: April 2024

The present study aimed to evaluate the evidence of validity and reliability of the Social Emotional Competence Questionnaire (SECQ) in Spanish students and to verify if gender is a determining factor in their development. An intentional sampling was carried out on 429 students between 12 and 16 years old. Three factorial structures were tested: the original model, the original re-specified model, and the six-factor model that was achieved. The results maintain that the original re-specified model presented values with the best absolute fit, a slightly more favorable comparative fit, and a parsimonious fit with lower values than the other models. The reliability analysis showed considerable internal consistency. Boys were shown to have better self-management, and girls showed greater management in their social relationships. It is concluded that the questionnaire has adequate psychometric properties and is expressly useful in the population of interest with a relevant weight in the evaluation of socio-emotional competence.
Digital Game-Based Heritage Education: Analyzing the Potential of Heritage-Based Video Games
Authors: Camuñas-García Daniel , Cáceres-Reche María Pilar , Cambil-Hernández María de la Encarnación , Lorenzo-Martín Manuel Enrique
Published: April 2024

Video games have emerged as a promising tool for enhancing engagement with cultural heritage. However, there is limited knowledge about how existing games can effectively fulfill this role. This study compiled and analyzed 100 video games based on cultural heritage by adapting an existing framework that includes 10 game attributes for a comparative statistical analysis of the games’ engagement features. These features include narrative-driven gameplay, information capsules, task-driven learning, ease of play, identity-driven content, open-world exploration, people-centered perspectives, meaningfulness, simulation, and verisimilitude. The analysis revealed that while the majority of games adhered to most of the recommended attributes, features such as task-driven learning, open-world exploration, and simulation were either uncommon or rare. These findings highlight a significant opportunity to develop games that incorporate these underrepresented features, thereby providing more immersive and engaging experiences in heritage education. Furthermore, the study offers a systematic overview of existing games that represent cultural heritage, serving as a valuable resource for developers, designers, and educators in this field.
DCGFuzz: An Embedded Firmware Security Analysis Method with Dynamically Co-Directional Guidance Fuzzing
Authors: Wang Yunzhi , Li Yufeng
Published: April 2024

Microcontroller Units (MCUs) play a vital role in embedded devices due to their energy efficiency and scalability. The firmware in MCUs contains vulnerabilities that can lead to digital and physical harm. However, testing MCU firmware faces challenges due to various tool limitations and unavailable firmware details. To address this problem, research is turning to fuzzing and rehosting. Due to the inherent imbalance in computational resources of the fuzzing algorithm and the lack of consideration for the computational resource requirements of rehosting methods, some hardware behavior-related paths are difficult to discover. In this work, we propose a novel Dynamically Co-directional Guidance Fuzzing (DCGFuzz) method to improve security analysis efficiency. Our method dynamically correlates computational resource allocation in both fuzzing and rehosting, computing a unified power schedule score. Using the power schedule score, we adjust test frequencies for various paths, boosting testing efficiency and aiding in the detection of hardware-related paths. We evaluated our approach on nine real-world pieces of firmware. Compared to the previous approach, we achieved a maximum increase of 47.9% in path coverage and an enhancement of 27.6% in effective model coverage during the fuzzing process within 24 h.
Light Intensity: A Key Ecological Factor in Determining the Growth of Pseudolarix amabilis Seedlings
Authors: Tong Jie , Ouyang Dawei , Wang Ji , Yan Xueqin , Fu Rurao , Chen Fusheng , Fang Xiangmin , Bu Wensheng , Lin Xiaofan , Li Jianjun
Published: April 2024

The notable absence of juvenile Pseudolarix amabilis trees in forest understories suggests their vulnerability to ecological niche competition, leading to limited survival prospects. This study examines the key factors limiting the growth of P. amabilis seedlings by investigating the effects of five ecological factors: light intensity, rainfall, groundwater level, soil type, and type of fertilization, on the growth of one-year-old P. amabilis seedlings. Our results demonstrate that increasing the light intensity promotes plant growth by augmenting the leaf count, leaf biomass, plant height, stem biomass, root biomass, and total biomass. Further analysis reveals that increased light intensity influences biomass allocation, reducing the specific leaf area and leaf–stem biomass ratio, and favoring root and stem growth over leaf investment. Rainfall, groundwater level, fertilization type, and rhizosphere soil type primarily influence root growth by impacting the soil’s physicochemical properties. Specifically, rising groundwater levels lower the soil temperature and increase the soil moisture, total potassium content, and soil pH, leading to reductions in root biomass, plant height, net height increment, leaf number, and total biomass. When groundwater levels reach 21 cm and 28 cm, submerging the surface soil layer, root biomass decreases by 1.6 g/plant (−51.6%) and 2.3 g/plant (−74.2%), respectively. Further analysis reveals a gradual decrease in the root–shoot ratio above the 14 cm groundwater level, while the specific leaf area and leaf–stem biomass ratio remains unaffected, indicating stronger belowground root stress compared to aboveground stem and leaf components. The results highlight light intensity as the key ecological factor determining the growth of P. amabilis seedlings. These findings underscore the importance of considering light intensity in the management of natural stands, the cultivation of artificial forests, and the nursery cultivation of endangered P. amabilis.
Investigation of Crypthecodinium cohnii High-Cell-Density Fed-Batch Cultivations
Authors: Dubencovs Konstantins , Suleiko Arturs , Suleiko Anastasija , Didrihsone Elina , Grube Mara , Shvirksts Karlis , Vanags Juris
Published: April 2024

Crypthecodinium cohnii is a marine microalga that can accumulate high amounts of polyunsaturated fatty acids (PUFAs) and thus replace conventional routes of fish oil production. They are associated with the destruction of marine resources and multiple downstream/purification complications. The major drawbacks of using C. cohnii for industrial-scale production are associated with low PUFA productivity. One of the means of increasing the PUFA synthesis rate is to maintain the medium component concentrations at optimal values throughout cultivation, thus increasing PUFA production efficiency, which can result in the successful transfer of the process to pilot and/or industrial scale. The goal of the present research was to develop techniques for increasing the efficiency of PUFA production via C. cohnii cultivation. Multiple experiments were carried out to test and fine-tune the cultivation medium composition and oxygen transfer factors. The biomass yields from individual components, yeast extract, sea salts, and glucose amounted to 5.5, 0.65, and 0.61 g·g−1, respectively. C. cohnii cell susceptibility to mechanical damage was experimentally evaluated. Power inputs of <276.5 W/m3 did not seem to promote cell destruction when Pitched-blade impellers were used. The obtained cultivation conditions were shown to be efficient in terms of increasing the biomass productivity and the omega-3 fatty acid content in C. cohnii. By using the applied methods, the maximal biomass productivity reached 8.0 g·L−1·day−1, while the highest obtained biomass concentration reached 110 g·L−1. A steady increase in the concentration of PUFAs during cultivation was observed from the FTIR data.
Effect of Chemical and Microbial Additives on Fermentation Profile, Chemical Composition, and Microbial Populations of Whole-Plant Soybean Silage
Authors: Gandra Jefferson Rodrigues , Takiya Caio Seiti , Del Valle Tiago Antonio , Pedrini Cibeli de Almeida , Gandra Erika Rosendo de Sena , Antônio Giovani , de Oliveira Euclides Reuter , Severo Igor Kieling , Rennó Francisco Palma
Published: April 2024

This study evaluated the effects of two chemical additives or a microbial inoculant on chemical composition and DM losses in whole-plant soybean silage. One-hundred and twenty mini-silos were used in a completely randomized design experiment with the following treatments: water without chloride (control, CON); a microbial inoculant (INO); a chemical additive containing 35–45% formic acid (FA type); and another chemical additive containing 50–60% propionic acid (PA type). Data were analyzed using mixed models of SAS, and treatment differences were evaluated by the following orthogonal contrasts: C1 = CON vs. additives (INO + FA type + PA type); C2 = INO vs. chemical additives (FA type + PA type); and C3 = PA type vs. FA type. Silage pH and ammonia nitrogen concentration were decreased, and concentrations of lactic acid and acetic acid were increased with additives. Counts of lactic acid bacteria were higher in silages with INO than with chemical additives. DM recovery increased with FA type and PA type. Additives increased DM and CP concentrations. Silage A-fraction proportion was greater with additives. Additives, particularly FA type and PA type, improved chemical composition and fermentative profile and reduced undigestible proportions of protein in whole-plant soybean silage. Chemical additives were more effective in reducing silage DM losses than INO.
Burn Severity and Postfire Salvage Logging Effects on Vegetation and Soil System in a Short-Term Period in Mediterranean Pine Forests
Authors: Peña-Molina Esther , Moya Daniel , Fajardo-Cantos Álvaro , García-Orenes Fuensanta , Mataix-Solera Jorge , Arcenegui Victoria , Lucas-Borja Manuel Esteban , de las Heras Jorge
Published: April 2024

Wildfires are a natural part of the dynamics of Mediterranean forest ecosystems. The fire patterns in the Mediterranean basin have been altered mainly due to changes in land use and climate change. In 2017, a wildfire in Yeste (Spain) burned 3200 hectares of two Mediterranean pine forests. We investigated the effects of burn severity and postfire salvage logging practices on vegetation and soil properties in four experimental areas distributed within the wildfire perimeter. These areas included unburned, low, high, and high burn severity with salvage logging, all located under Pinus halepensis Mill and Pinus pinaster Aiton stands. Salvage logging was applied 18 months after the fire. We established 72 circular plots (nine per treatment and pine species). We collected soil samples to analyze physicochemical and biological soil properties, including pH, electrical conductivity (EC), soil organic matter (SOM) content, carbon from microbial biomass (CBM), basal soil respiration (BSR), metabolic quotient (qCO2), and two enzymatic activities: β-glucosidase (GLU) and phosphatase (PHP). To understand how vegetation changed after fire, we implemented three linear transects per plot to calculate α-diversity indices (richness, Shannon, and Simpson), vegetation coverage (COBV), fraction of bare soil (BSOIL), the number of postfire seedlings (NSeed) and their average height (Hm), and we grouped vegetation into different postfire adaptive strategies: facultative seeder (R+S+), obligate resprouter (R+S−), obligate seeder (R−S+), and non-fire-adapted (R−S−). We ran ANOVA and Tukey’s HSD post hoc tests to evaluate the differences between burn severity and salvage logging practices on the variables examined for each pine stand. We used PCA and correlation analysis to identify plant-soil interactions. Our results suggest that Pinus halepensis stands were more affected by the wildfire than Pinus pinaster stands due to the distinct characteristics of each species (morphology of the leaves, bark thickness, cone structure, etc.) and the significant differences observed in terms of pH, SOM, CBM, qCO2, GLU, PHP, and Nseed. The proportion of obligate resprouter species was higher in Pinus halepensis stands, and the obligate seeder species were higher in Pinus pinaster stands. The study highlighted the importance of monitoring burn severity and postfire management practices to promote forest recovery and reduce wildfire risk. Limiting the negative impact of postfire salvage logging practices can enhance the resilience of vulnerable ecosystems.