bluuebunny/crossref_metadata_2025 huggingface dataset:
Penyuluhan Pemanfaatan Limbah Pertanian Untuk Pakan Ternak Alternatif Menggunakan Mesin Pencacah Rumput Multifungsi dengan Proses Amoniase pada Kelompok Ternak Makmur Desa Kebondalem, Jombang
Authors: Fatkhurrohman  , Mufti Mohammad , Fathurahman Mohamad Rifan
Published: January 2023

Kuantitas pakan ternak di Desa Kebondalem berupa rumput hijau saat musim hujan sangat melimpah, namun saat musim kemarau kuantitasnya sangat sedikit. Disisi lain, saat musim kemarau limbah pertanian seperti jerami padi, bonggol jagung dan pohon kedelai kuantitasnya sangat melimpah tetapi belum sepenuhnya dimanfaatkan oleh kelompok ternak Makmur sebagai pakan ternak. Pemanfaatan limbah pertanian dapat ditingkatkan kembali dengan membuat pakan ternak alternatif dari limbah pertanian sebagai upaya pemenuhan kuantitas pakan ternak. Metode amoniase dipilih untuk pembuatan pakan ternak alternatif karena hemat biaya dan mudah dilakukan. Kebutuhan pakan ternak kelompok ternak Makmur mencapai 600 kg/hari sehingga dalam proses pencacahan limbah pertanian sebelum pembuatan amoniase dilakukan menggunakan mesin pencacah rumput multifungsi. Mesin yang digunakan mempunyai kapasitas 500 kg/jam. Berdasarkan perhitungan didapatkan bahwa untuk memenuhi pakan ternak selama musim kemarau (6 bulan) dibutuhkan pakan sebanyak 108.000 kg, dengan menggunakan mesin pencacah rumput multifungsi dibutuhkan waktu 27 hari untuk memenuhi kebutuhan pakan ternak selama 6 bulan dengan estimasi penggunaan mesin yaitu 8 jam/hari. Kegiatan penyuluhan pengoperasian mesin dan pembuatan pakan ternak alternatif metode amoniase telah dilakukan. Dari perhitungan yang dilakukan dan kesiapan kelompok ternak dalam menerima materi penyuluhan yang diberikan, sangat besar kemungkinan masalah terkait kuantitas pakan ternak yang sedikit di musim kemarau sudah terselesaikan.
Pengembangan Kapasitas Produksi Merchandise Desa Wisata Edukasi Rawagede Berbasis Pembelajaran Hyflex
Authors: Wongsonadi Sri Koeswantono , Sasmita Karta , Hikmah  , Hadiyanti Puji , Hutama Nararia
Published: January 2023

Pengabdian ini merupakan bentuk penerapan dari roadmap pengabdian yang telah disusun berdasarkan  keadaan desa binaan yang dilakukan oleh prodi Pendidikan Masyarakat Fakultas Ilmu Pendidikan.  Roadmap kegiatan pengabdian ini dilaksanakan selama 3 tahun. Tahun pertama melakukan kegiatan  pengabdian dengan materi Pengembangan Kapasitas Produksi Merchandise Desa Wisata Edukasi  “Rawagede” berbasis Pembelajaran HyFlex. Tahun kedua membangun usaha kelompok yang  berkelanjutan, materi kegiatan pengabdian berfokus pada jejaring kemitraan usaha. Tahun ketiga berfokus  untuk melihat dampak setelah adanya Pengembangan Kapasitas Produksi Merchandise Desa Wisata  Edukasi “Rawagede” berbasis Pembelajaran HyFlex, materi yang diberikan adalah pendampingan usaha  Merchandise berkelanjutan. Pengabdian ini dilatarbelakangi oleh potensi Desa wisata Sirna Jaya sebagai  aset mengembangkan human capital dan social capital masyarakat. Melalui desa wisata, banyak  masyarakat terlibat dalam bersinergis mengembangkan desa wisata. Produk wisata merupakan bagian  dari penerapan sapta pesona yang berwujud kenangan, kenangan tersebut diwujudkan dalam bentuk  cinderamata. Harapannya cinderamata mampu menjadi kenangan yang berwujud bagi wisatawan yang  berkunjung. Kegiatan pembelajaran HyFlex sendiri memberikan level flexibilitas baru untuk blended  learning, metode pembelajaran ini memberikan kesempatan bagi warga belajar untuk memilih apakah  akan menghadiri pembelajaran secara daring, luring atau keduanya. Luaran pengabdian berbentuk  publikasi artikel pada jurnal nasional, video kegiatan pada kanal Youtube dan booklet kegiatan yang dapat  di hak patenkan.
Politik Hukum dalam Legislasi Nasional
Authors: Hariyanto Hariyanto
Published: January 2023

In general, the product of legislation is a work that is normative. So that everything that is normative is open to legal deviations. This potential deviation from the law will create what is called a legal error (legal gap). Therefore, this paper aims to formulate the character of legal products that are responsive and participatory (democratic) in the reform era. This paper is a library research or normative legal research. The approach used is a normative juridical approach. Data sources such as primary legal materials related to the issue of law formation. While secondary legal materials such as books, journals, and all secondary materials related to the focus of the study. The analysis uses the doctrinal method. This paper concludes that the responsive and participatory character of legal products in the reform era requires a public space that is free from the interests of political elites and power. There needs to be civil society involvement in the formation of laws and regulations, starting from the stage of drafting academic texts to public notification before the ratification of the legislation.

Pada umumnya produk legislasi merupakan sebuah karya yang bersifat normatif. Sehingga semua yang bersifat normatif ini terbuka sekali terjadinya penyimpangan-penyimpangan hukum. Potensi penyimpangan dari hukum ini akan mencipatakan apa yang disebut sebagai kekosongan hukum (legal gap). Oleh karena itu, tulisan ini bertujuan merumuskan karakter produk hukum yang responsif dan partisipatif (demokratis) dalam era reformasi. Tulisan ini merupakan penelitian kepustakaan (library research) atau penelitian hukum normatif. Pendekatan yang digunakan adalah pendekatan yuridis normatif. Sumber data seperti bahan hukum  primer yang terkait dengan isu pembentukan hukum. Sedangkan bahan hukum sekunder seperti  buku, jurnal, dan semua bahan sekunder yang terkait dengan fokus kajian. Analisis menggunakan metode doktrinal. Tulisan ini menyimpulkan bahwa karakter produk hukum yang responsif dan partisipatif dalam era reformasi diperlukan ruang publik yang bebas dari kepentingan elit politik dan dominasi kekuasaan. Perlu adanya keterlibatan masyarakat (civil society) dalam pembentukan peraturan perundang-undangandari menyusun naskah akademik hingga mengadakan public hearing sebelum undang-undang disahkan
Kesadaran Hukum Masyarakat Muslim Pesisir dalam Mengeluarkan Zakat di Desa Wedung Kecamatan Wedung Kabupaten Demak
Authors: Shobirin Shobirin , Abdullah Junaidi
Published: January 2023

This research was conducted because the majority religion in Wedung Village Wedung District Demak Regency is Islam. However, zakat is only prioritized to be given to religious leaders, so the poor are excluded. The purpose of this study was to determine the legal awareness of the coastal Muslim community regarding issuing zakat, and the factors that influence it. This type of research is empirical juridical with a qualitative approach. Primary data sources were obtained directly from interviews with informants in Wedung Village, and secondary data sources were obtained from relevant books and journals. All data or information obtained is then processed using descriptive data analysis techniques. The results of the research conclusions show that coastal communities in Wedung Village Wedung District Demak Regency have a good level of awareness in issuing zakat fitrah and maal every year because they consider issuing zakat as a religious obligation even though issuing zakat is not through LAZ and BAZNAS. Meanwhile, the factors that influence the formation of community awareness in Wedung Village to issue zakat are knowledge from madrasa benches, recitation assemblies, sermons, or lectures by clerics. Stages of change Legal awareness related to issuing zakat is manifested in the form of choices, actions, goals, and utilization of the local community.

Urgensi Penelitian ini dilakukan karena agama mayoritas di Desa Wedung Kecamatan Wedung Kabupaten Demak adalah Islam. Namun demikian, zakat hanya diutamakan diberikan kepada tokoh agama, sehingga orang-orang miskin dikesampingkan. Tujuan Penelitian ini adalah untuk mengetahui kesadaran hukum masyarakat Muslim Pesisir terhadap mengeluarkan zakat, dan faktor yang mempengaruhinya. Jenis penelitian ini adalah yuridis empiris dengan pendekatan kualitatif. Sumber data primer didapatkan secara langsung wawancara dengan para informan Desa Wedung, dan sumber data sekunder berasal dari buku maupun jurnal yang relevan. Semua data atau informasi yang didapat selanjutnya diolah dengan menggunakan teknik analisis data berupa deskriptif. Hasil kesimpulan penelitian menunjukkan bahwa masyarakat pesisir di Desa Wedung Kecamatan Wedung Kabupaten Demak memiliki tingkat kesadaran yang baik dalam mengeluarkan zakat fitrah dan maal setiap tahunnya sebab menganggap mengeluarkan zakat sebagai sebuah kewajiban beragama meskipun dalam mengeluarkan zakat tidak melalui LAZ dan BAZNAS. Sementara itu faktor-faktor yang mempengaruhi terbentuknya kesadaran masyarakat Desa Wedung mengeluarkan zakat yaitu pengetahuan dari bangku madrasah, majelis-majelis pengajian, khutbah atau ceramah para kyai. Tahapan perubahan Kesadaran hukum terkait mengeluarkan zakat diwujudkan dalam bentuk pilihan, tindakan, sasaran dan pemanfaatan dari masyarakat setempat.
THE IMPACT OF COVID-19 VACCINATION ON DEVELOPMENT WORLD ECONOMY: SOCIO-ECONOMIC ASPECT
Authors: Yakushenka. K.
Published: January 2023

The article is devoted to the study of the process of vaccination against coronavirus
infection on the development of the world economy in order to consider the stimulating
measures and conditions necessary for the restoration of economic activity and growth. The dynamics of the implementation of vaccination campaigns is given, the analysis of the key global players in the vaccine market is carried out. Social and economic trends affecting the vaccination process have been identified (increased risk of uneven access to vaccinations and their distribution; refusal of certain categories of citizens from vaccination due to the influence of the processes of economic and informational uncertainty; immunological inequality; lack of a single vaccine space). Evidence of the impact of vaccination against COVID-19 on the development of the world economy from the point of view of socioeconomic approach is given.
ECONOMIC THEORY AND ETHICS
Authors: Kuregyan. S.
Published: January 2023

The article is devoted to the study of modern problems of economic theory, which is understood as the synthesis of political economy and economics. Among the urgent problems of economic theory, it is necessary to single out, first of all, the problem of man, as well as the influence of ethics on economic relations, that is, the subject of economic theory.
Analisis Framing Berita Pencalonan Kandidat Di Portal Berita Online Lokal Di Pilkada Pacitan 2020
Authors: Hendriyanto Agoes , Purwasito Andrik , Rais Wakit Abdullah , Hastjaryo Sri
Published: January 2023

Penelitian  analisis framing   berita pencalonan kandidat di portal berita  online lokal di pilkada Pacitan 2020. Tujuan penelitian  untuk melihat bagaimana berita pencalonan kandidat di portal berita online lokal yakni www.pacitanku.com dan www.lintas7.net di pilkada Pacitan 2020.  Penelitian kritis  dengan analisis framing Entman dengan seleksi isu dan penekanan serta penonjolan di teks berita.  Hasil penelitian sebagai berikut. Pertama, politikus santun dengan penonjolan; Gagarin kader santun dan  Sudijono politikus santun. Kedua, kader rakyat,   dengan penonjolan Gagarin kader rakyat. Ketiga, pengusaha suskes dengan penonjolan Winarni pengusaha sukses. Keempat, Guru besar dengen penonjolan Sudijono sang guru. Kelima, Pembangunan Sektor Agraris dan Wisata; dengan penonjolan Roni Wahyono rencanakan pembangunan dengan ahli dan pembangunan agrowisata.
A Neonatal Sepsis Prediction Algorithm Using Electronic Medical Record (EMR) Data from Mbarara Regional Referral Hospital (MRRH)
Authors: Ezeobi Dennis Peace , Musiimenta Angella , Wasswa William , Kyoyagala Stella
Published: January 2023

BackgroundNeonatal sepsis is a significant cause of neonatal death and has been a major challenge worldwide. The difficulty in early diagnosis of neonatal sepsis leads to delay in treatment. The early diagnosis of neonatal sepsis has been predicted to improve neonatal outcomes. The use of machine learning techniques with the relevant screening parameters provides new ways of understanding neonatal sepsis and having possible solutions to tackle the challenges it presents. This work proposes an algorithm for predicting neonatal sepsis using electronic medical record (EMR) data from Mbarara Regional Referral Hospital (MRRH) that can improve the early recognition and treatment of sepsis in neonates.Methods A retrospective analysis was performed on datasets composed of de-identified electronic medical records collected between 2015 to 2019. The dataset contains records of 482 neonates hospitalized in Mbarara Regional Referral Hospital, Uganda. The proposed algorithm implements Support Vector Machine (SVM), Logistic regression (LR), K-nearest neighbor (KNN), Naïve Bayes (NB), and Decision tree (DT) algorithms, which were trained, tested, and compared based on the acquired data. The performance of the proposed algorithm was evaluated by comparing it with the physician's diagnosis. The experiment used a Stratified K-fold cross-validation technique to evaluate the performance of the models. Statistical significance of the experimental results was carried out using the Wilcoxon Signed-Rank Test. ResultsThe results of this study show that the proposed algorithm (with the lowest Sensitivity of 95%, lowest Specificity of 95%) outperformed the physician diagnosis (Sensitivity = 89%, Specificity = 11%). SVM model with radial basis function, polynomial kernels, and DT model (with the highest AUROC values of 98%) performed better than the other models in predicting neonatal sepsis as their results were statistically significant.ConclusionsThe study provides evidence that the combination of maternal risk factors, neonatal clinical signs, and laboratory tests effectively diagnose neonatal sepsis. Based on the study result, the proposed algorithm can help identify neonatal sepsis cases as it exceeded clinicians' sensitivity and specificity. A prospective study is warranted to test the algorithm's clinical utility, which could provide a decision support aid to clinicians.
Experimental study on a novel double Y-shaped filter and its dust removal characteristics
Authors: Ji Ben , Jiang Bingyou , Yuan Liang , Ren Bo , Zheng Yuannan , Su Mingqing , Zhu Zhihui , Lin Hanyi , Yu Changfei , Zhu Yufeng
Published: January 2023

For efficient and energy-saving purification of underground coal dust, a double Y-shaped filter device with a pneumatic fan as the power source was presented and tested. Numerical simulation results showed that the dust removal resistance of the double Y-shaped filter was lower than that of the conventional parallel filter. Next, filtration performance was evaluated and the morphology of water film on the filter was analyzed. It was discovered that a moderate filtration velocity was beneficial in increasing dust removal efficiency and the density of the fiber grid was proportional to the dust removal efficiency of the equipment. Conversely, an excess filtration velocity will increase the dust removal resistance and decrease the dust removal performance. According to quality factor evaluation, the optimum performance parameters for this equipment were 60 mesh density fiber grid, 0.6 MPa spray pressure, and 5 m/s filtration velocity. Under these conditions, the dust removal efficiency was 98.2%, and the dust removal resistance was 169.6 Pa. The results obtained in this study showed that this device can provide a new idea for efficient, low resistance and dust removal without electricity in underground coal mines.
Study on PM diffusion and distribution of trackless rubber-tyred vehicle under different driving conditions in underground coal mining environment
Authors: Nie Wen , Liu Chengyi , Hua Yun , Bao Qiu , Niu Wenjin , Jiang Chenwang
Published: January 2023

Particulate matter (PM) is one of the most harmful exhaust pollutants to human health. In this study, the PM diffusion and distribution emitted by trackless rubber-tyred vehicle under different driving conditions in coal mine was analyzed with numerical simulations and field measurements. The results show that when the vehicle velocity was constant, the proportion of PM with a concentration below 6 mg/m3 was the highest owing to the influences of diffusion and airflow dilution. However, when the diffusion distance is less than 3 m, the PM concentration far exceeds the occupational exposure limit (C = 10 mg/m3) specified in the coal mine safety regulations.With increasing vehicle velocity, the PM concentration gradient at a diffusion distance of 0–6 m showed the most significant slope. Besides, the concentration fluctuation of PM was the largest and relatively high when the diffusion distance was 5–15m. Therefore, the area 15 m from the exhaust gas pipe opening of the trackless rubber-tyred vehicle should be controlled.
Global Evolutional Trend of Safety in Coal Mining Industry. A Bibliometric Analysis
Authors: APPIAH AUGUSTINE , Li Zhigang , Ofori Elvis Kwame , Mintah Clement
Published: January 2023

Mining safety is recognized as one of the factors influencing the mining industry's long-term viability. Therefore we did a bibliometric analysis to take stock of safety management in the coal mining industry. This study suggests a three-step strategy, comprising literature extraction and screening, bibliometric analysis, and discussion, to provide an in-depth understanding of the present state and development trend of mine safety research. The discovery raises concerns. (i) Coal dust pollution has a direct and indirect impact on the environment. (ii) Most research projects have prioritized technology innovation and development over safety norms. (iii) Most works have come from advanced countries such as China, the United States of America, the United Kingdom and Australia to the neglect of developing nations, leaving a significant vacuum in the literature. (iv) There are more major safety principles in the food business than in the mining industry, indicating a weak safety culture in the mining industry. Additionally, future research goals are provided, such as creating safer policy guidelines to support technological advancements, constructing effective safety mines, and creating solutions to dust pollution and human errors.
Comparison of Quality Performance Metrics in Screening and Surveillance Colonoscopy: A Single-Center Experience
Authors: Love James Stephen , Siegel Michael , Yellen Meredith , Rebhun Jeffrey , Shuja Asim
Published: January 2023

Background and aims:
Current guidelines for screening colonoscopies recommend a minimum adenoma detection rate (ADR) of 25%. There are no established benchmarks for surveillance colonoscopies and data surrounding the utility of other quality metrics in this setting is limited. We aimed to define the relationship between ADR and alternative quality measures in the setting of screening and surveillance colonoscopies and determine whether validated screening quality benchmarks can be extrapolated to surveillance procedures.
Methods:
A retrospective review was conducted of adults who underwent outpatient screening and surveillance colonoscopies at a tertiary health center. ADR, adenomas per colonoscopy (APC), adenomas per positive participant (APP), polyp detection rate (PDR), right-sided polyp detection rate (RSP), and withdrawal times (CWT) were analyzed for screening and surveillance colonoscopies. Normality tests were performed for each continuous variable. Kruskal-Wallis tests were performed to analyze categorical and continuous outcomes. Spearman rank correlations were performed to compare surrogate outcomes to ADR.
Results:
In total, 2646 procedures, 1884 screening and 762 surveillance, were analyzed. Surveillance ADR (CADR) was significantly higher than screening ADR (SADR) (65.6%+0.02 v. 47.0%+0.01%, p<0.001). All alternate quality measures except CWT were significantly higher in surveillance procedures. Among surveillance procedures, there was a strong correlation between CADR and PDR (r=0.956, p<0.01), as well as RSP (r=0.771, p=0.003); correlations between CADR and other alternate quality measures were not significant.
Conclusion:
Colonoscopy quality measures were significantly higher in surveillance procedures when compared to screening procedures despite similar CWT. Higher benchmarks must be established to ensure quality surveillance colonoscopies.
Measurement and influencing factors of industrial carbon unlocking efficiency under the background of "double carbon"
Authors: he yanzi , Wang Wanyu , li haibo
Published: January 2023

Based on the panel data of 11 provinces and cities in the Yangtze River Economic Belt from 2011 to 2018, the DEA model and Malmquist index were used to calculate the industrial carbon unlocking efficiency of the Yangtze River Economic Belt in a static and dynamic way, and the Tobit model was used to empirically test its influencing factors. The results show that:(1)Generally speaking, the industrial carbon unlocking efficiency of the Yangtze river economic belt is on the rise, but compared with the upper and middle reaches of the Yangtze river, the industrial carbon unlocking efficiency of the lower reaches of the Yangtze river has certain advantages. The total factor productivity of industrial carbon unlocking in the Yangtze River Economic Belt has increased steadily on the whole, and the main source of growth is technological progress.༈2༉Industrial carbon unlocking efficiency is affected by many factors. Environmental regulation and opening to the outside world have a promoting effect on industrial carbon unlocking efficiency, while government investment and innovation level have a restraining effect on industrial carbon unlocking efficiency, and the effect of human capital level is not significant.
Symptomatic intracranial hemorrhage mediates the association between eosinophils and 90-day outcomes after mechanical thrombectomy for acute ischemic stroke
Authors: Yu Shuhong , Wang Xiaocui , Huang Hao , Luo Yi , Guo Zhiliang
Published: January 2023

Background
Decreased eosinophil level was associated with poor outcome after mechanical thrombectomy in patients with acute ischemic stroke (AIS), but pathogenesis of this association is elusive. We aimed to assess the mediation effect of intracranial bleeding complications on the aforementioned association.
Methods
A total of three hundred and twenty-eight consecutive AIS patients experiencing mechanical thrombectomy between May 2017 and March 2021 were analyzed. Hemorrhagic transformation (HT) were categorized as symptomatic intracranial hemorrhage (sICH) and parenchymal hematoma (PH) according to previously published criteria. Regression analysis was used to assess the effect of eosinophils on HT, and its effect on poor outcome. Mediation analysis was utilized to assess the proportion of total effect by HT on the association between eosinophils and poor outcome.
Results
Multivariater analysis revealed that eosinophils was independently associated with sICH after adjusting for potential confounders (odds ratio, 0.00; 95% CI, 0.00–0.01; P = 0.0141), which is consistent with the result of eosinophils (dichotomous) as a categorical variable (odds ratio, 0.22; 95% CI, 0.11–0.46; P < 0.0001). And the risk of PH in higher eosinophils was 0.36 fold higher than in patients in the low eosinophil group (OR, 0.36, 95% CI, 0.19–0.67; P = 0.0013). Eosinophils was negative associated with poor outcome (odds ratio, 0.00; 95% CI, 0.00–0.02; P = 0.0021). And mediation analysis found that sICH partially mediated the negative relationship between eosinophils and poor outcome (indirect effect=-0.1896; 95%CI: -0.3654 – -0.03, P < 0.001); however, PH did not mediate the association between eosinophils and poor outcome (P = 0.12).
Conclusion
This study showed an important effect of sICH on the association between eosinophils and poor outcome.
Global prevalence of self-harm during the COVID-19 pandemic: A systematic review and meta-analysis
Authors: Cheng Huan , Wang Lu , Zou Haiou , Qu Yanhua
Published: January 2023

Background: Little is know about the prevalence of self-harm worldwide during the COVID-19. This study aimed to estimate the prevalence of self-harm worldwide during COVID-19. An attempt was also performed to explore the causes of heterogeneity by analyzing the characteristics in the included studies.
Methods: By using permutations of COVID-19, self-harm or relevant search terms, we searched the following electronic databases for studies prior to January 2022: Web of Science, PubMed, MEDLINE, Embase, PsycINFO, Cochrane Database of Systematic Reviews, China National Knowledge Infrastructure (CNKI), Wanfang Database and systematically reviewed the evidence according to MOOSE guidelines. We employed the Cochran’s chi-squared test (Cochran’s Q), I2 test and subgroup analysis to assess and address the heterogeneity. Funnel plots, Egger’s and Begg’s tests were utilized to evaluate publication bias, while trim-and-fill method was done to identify possible asymmetry and determine the robustness of the results.
Results: 16 studies that met the inclusion and exclusion criteria were identified, with the sample sizes ranging from 228 to 49,227. The methodological quality of the included studies were mostly at the medium level. By using random effect model, the pooled prevalence of self-harm was 15.8% (95%CI 13.3-18.3). Based on subgroup analysis, the following characteristics of the included studies reported a higher prevalence of self-harm: studies conducted in Asia or prior to July 2020, cross-sectional studies, samples recruited in hospital or school, adolescents, female, the purpose of self-harm (NSSI), mental symptoms and restriction experiences.
Conclusions: We provide first meta-analytic estimated prevalence of self-harm based on large sample from different countries and populations. The incidence of self-harm during COVID-19 was not encouraging and requires attention and intervention. The obvious heterogeneity between included studies requires more high-quality and prospective studies in the future to obtain a more accurate prevalence of self-harm. In addition, this study also provides new directions for future research, including the identification of high-risk groups for self-harm, the formulation and implementation of prevention and intervention programs, and the long-term impact of COVID-19 on self-harm, etc.
Carbon dioxide emissions, economic growth, energy usage, trade openness and foreign direct investment: Evidence from G8 Countries
Authors: AYDIN Fatma Fehime
Published: January 2023

This study aims to examine the effects of economic growth, energy usage, trade openness and foreign direct investment on carbon dioxide (CO2) emissions for G8 countries using annual data for the period 1990–2018. For this purpose, this study primarily follows the cross-section dependence and heterogeneity tests. Then, unit root and cointegration tests, cointegration analyzes and causality analyzes are performed in the study. Finally, the article estimates short-term parameters and long-term parameters to capture possible dynamic relationships between variables. The Westerlund Error Correction Model (ECM) panel test for cointegration shows that there is a cointegration relationship between these variables for both the entire panel and the cross-section units. Augmented Mean Group (AMG) estimator method shows that economic growth has no effect on CO2 emissions in the majority of the countries studied, energy usage increases CO2 emissions, while foreign direct investments and trade openness do not affect CO2 emissions in some countries, but positively effects in some countries and negatively in others. According to the results obtained from the Pooled Mean Group (PMG) analysis, it has been determined that economic growth, energy usage and trade openness are important to explain the change in CO2 emissions in the long run, while energy usage and trade openness are important to explain the change in CO2 emissions in the short run too, but economic growth is not. According to Dumitrescu Hurlin panel causality results, it is seen that there is no causal relationship between CO2 emissions, economic growth and energy use. While there is a unidirectional causality from CO2 emissions to foreign direct investments, it has been determined that there is a bidirectional causality between trade openness and CO2 emissions. When the results are examined in general, this study provides important ideas about the determinants of CO2 emissions in the G8 countries.
Epidemiology and Genomic characteristics of Wenzhou viruses in rodents from the southeast coast of China
Authors: Qinghua Xie , Zhu Changqiang , Ai Lele , Nie Danyue , Wu Yifan , Wang Chongcai , He Ji , Tan Weilong , Zhang Lingling
Published: January 2023

Background: Wenzhou virus (WENV), a member of the Mammarenavirus genus in the Arenaviridae family, has been detected in wild rodents from eight provinces in China and Southeast Asia. The IgG-antibodies to WENV have been detected in healthy people and patients with unknown fever andrespiratory symptoms. However, since WENV was discovered, its potential harmfulness has been underestimated due to its mild symptoms after human infection, similar to respiratory diseases. Thus, it is imperative to further understand the pathogenicity of WENV and its mechanisms, to improve WENV surveillance in wild rodents, particularly Rattus norvegicus, and to detect the prevalence of WENV as needed.
Results: During 2017-2021, a total of 390 wild rodents were collected from six provinces in eastern and southern coastal areas, containing nine species of rats, of which 36.4% were Rattus norvegicus. Samples of each tissue were collected and PCR amplification was performed. Four WENV-positive samples were detected, all from Rattus norvegicus. No genomic sequence of WENV was detected in Rattus flavipectus, Rattus losea, Suncus murinus, Apodemus agrarius, Mus musculus, Microtus fortis, Micromys minutus, and Niviventer niviventer from Jiangsu, Zhejiang, Fujian, Hainan, Guangdong and Guangxi Provinces. The full-length strain of WENV (HNHKW40/2020) was amplified from Rattus norvegicus in Hainan, which was most closely related to Wufeng/ WFS. The nucleotide sequence homology was 84.5-89.4% and the amino acid sequence homology was 91.6-98.9%. The sequences obtained in Xiamen, Fujian Provinces had more than 90% homology with WENV, which may be a strain of WENV. 83% of the positive sequences detected in Wuxi, Jiangsu Province were homologous to Lassa virus (LASV), which may be a member of LASV. Phylogenetic analysis indicated that all WENVs clustered together to form the Old World group with LASV and Loei River virus (LORV).
Conclusions: In this study, we systematically examined the prevalence of WENV amongrodents inthe southeast coast of China and characterized the genome of a newly discovered WENV strain, confirming that rodents of Rattus norvegicus played a main role in WENV transmission. The need to enhance WENV surveys in wild rodents and humans was emphasized.
Effects of self-directed learning on clinical competence: The mediating role of clinical learning environment among internship nursing students
Authors: Vasli Parvaneh , Asadiparvar-Masouleh Hanieh
Published: January 2023

Introduction: It is crucial to recognize the factors affecting the clinical competence of internship nursing students. This study aimed to determine the effects of self-directed learning on the clinical competence of internship nursing students with the mediating role of the clinical learning environment.
Methods: This cross-sectional research was performed on 300 internship nursing students selected by convenience sampling. Data were collected in one stage using three tools of Self-Directed Learning Readiness Scale for Nursing Education, Education Environment Measure, and Clinical Competence Questionnaire. Data analysis was performed in SPSS version 21 and Smart-PLS version 3 using Partial least squares-SEM with considering a p-value of 0.05.
Results: The results showed that 20.5% of the variance in clinical competence could be explained by self-directed and clinical learning environments. Self-directed learning had a positive and significant effect on clinical competence (path coefficient=0.14, 95% CI: 0.02, 0.26; p=0.027), and on clinical learning environment as well (path coefficient=0.41, 95% CI: 0.31, 0.52; p<0.001). A relationship was reported between clinical learning environment and clinical competence (path coefficient=0.38, 95% CI: 0.25, 0.50; p<0.001). The indirect effect of self-directed learning on clinical competence was positive and significant (path coefficient=0.11, 95% CI: 0.07, 0.17; p<0.001). Self-directed learning has a significant total effect on clinical competence (path coefficient=0.30, 95% CI: 0.19, 0.40; p<0.001).
Conclusions: It is recommended that nursing education managers and instructors consider some plans to enhance self-directed learning among nursing students and improve the clinical learning environment.
Methyl jasmonate and β-cyclodextrin shake hands to boost withaferin A production from the hairy root culture of Withania somnifera
Authors: Karami Mahbobeh , Naghavi Mohammad Reza , Nasiri Jaber , Farzin Narjes
Published: January 2023

For the first time, an effort was made to study potential effects of leaf and stem explants of three genotypes of Withania somnifera (L.) Dunal (i.e., G1, G2, and G3) for hairy root induction via applying four strains of Agrobacterium rhizogenes (i.e., LBA 9402, A4, and ATTC 15834) and A. tumefaciens (i.e., C58C1). Furthermore, to improve withaferin A production, the superior treatment was subjected to elicitation with β-cyclodextrin (β-CD) and methyl jasmonate (MeJA) (alone or in combination) after 48 h exposure time. Among 24 treatments resulted from multiplying three main factors, the superiority of “G3/ATCC/LEAF” treatment was verified in terms of “induction rate” and “number of induced roots” alongside “growth curve”, and utilized subsequently for elicitation assay. The highest amounts of withaferin A were quantified for both hairy root sample groups of “0.5 mM β-CD + 100 µM MeJA” (9.57 mg/g DW) and “5.0 mM β-CD + 100 µM MeJA” (17.45 mg/g DW), with the fold change values of 6.84-fold and 12.46-fold compared to control, respectively. Similarly, either individual or simultaneous applications of both elicitors, apart from some fluctuations, could overall boost transcript magnitudes of the four selected genes of HMGR, SQS, SMT-1, and SDS/CYP710A involved in withanolides biosynthetic pathway as compared with control. The results, altogether, demonstrated the superiority of leaf explant and Agropine-type strain of ATCC 15834 for hairy root induction, followed by synergistic effect of exogenous application of both MeJA and β-CD on the in vitro production of withaferin A, and possibly other types of withanolides in the hairy root culture of W. somnifera.
His-Purkinje System Pacing versus Biventricular Pacing in clinical efficacy: A Systematic Review and Meta-Analysis
Authors: Wang Ya , Liu Fangchao , Liu Mengyao , Wang Zefeng , Lu Xiangfeng , Huang Jianfeng , Gu Dongfeng
Published: January 2023

Background
His-Purkinje system pacing (HPSP), including His-bundle pacing (HBP) and Left bundle branch pacing area (LBBaP), imitates the natural conduction of the heart as an alternative to biventricular pacing (BVP) in cardiac resynchronization therapy (CRT). However, current evidence on the feasibility and efficacy of HPSP were from studies with limited sample size, and the aim of this study was to provide a comprehensive assessment through a systematically review and meta-analysis.
Methods
PubMed, EMBASE, Cochrane Library and Web of Science database were searched from inception to October 27, 2022 to compare the clinical outcomes associated with HPSP and BVP in patients for CRT. Clinical outcomes of interest including QRS duration (QRSd), left ventricular (LV) function and New York Heart Association (NYHA) functional classification, pacing threshold, echocardiographic and clinical response and hospitalization rate of HF were also extracted and summarized for meta-analysis.
Results
A total of 12 studies (nine observational studies and three randomized studies) involving 644 patients were included. The patients were matched on age and sex and were followed for 6–24 months. Compared with BVP, CRT patients treated by HPSP had shorter QRSd [mean difference (MD): -28.03 ms, 95% confidence interval (CI): -35.54 to -20.53, P༜0.001, I2 = 85%], greater LV functional improvement with increased left ventricular ejection fraction (LVEF) (MD: 5.81, 95% CI: 4.24 to 7.38, P༜0.001, I2 = 0%) and decreased left ventricular end-diastolic dimension (LVEDD) (MD: -4.05, 95% CI: -5.89 to -2.20, P༜0.001, I2 = 0%), more improved NYHA functional classification (MD: -0.45, 95% CI: -0.67 to -0.23, P༜0.001, I2 = 70%). In addition, HPSP were more likely to have higher echocardiographic [odds ratio (OR): 2.76, 95% CI: 1.74 to 4.39, P < 0.001, I2 = 0%], clinical (OR: 2.10, 95% CI: 1.16 to 3.80, P = 0.01, I2 = 0%) and super clinical (OR: 3.17, 95% CI: 2.09 to 4.79, P < 0.001, I2 = 0%) responses than BVP, while there was no difference in the hospitalization rate of HF (OR: 0.34, 95% CI: 0.10 to 1.17, P = 0.09, I2 = 0%) between them. When considering threshold change, HBP was less stable than LBBaP (MD: -0.16 V, 95% CI: -0.25 to -0.06, P = 0.0009, I2 = 35%), but had no difference with BVP (MD: 0.11 V, 95% CI: -0.09 to 0.31, P = 0.28, I2 = 0%).
Conclusion
Our findings suggested that HPSP was associated with more remarkable improvement of cardiac function in patients with indication for CRT and a potential alternative to BVP to achieve a physiological pacing through native his-purkinje system.
Prevalence of Antibiotic Residues, Antibiotic Resistance Bacteria and Antibiotic Resistance Genes in Hospital Wastewater: Silent threat to Human Health
Authors: Liyanage Gayani Yasodara , Weerasekara Manjula , Manage Pathmalal Marakkale
Published: January 2023

Hospital wastewater tends to be a hotspot for the environmental spread of antibiotic resistance, as recent studies have shown that antibiotic residues, resistant bacteria, and resistant genes occur in aquatic environments. The present study reports antibiotic residues, bacteria that are resistant to antibiotics, and genes associated with antibiotic resistance in hospital wastewater in Sri Lanka. The highest concentration of AMX (0.001-0.024 µg/ml) and AMP (0.001-0.023 µg/ml) among the selected antibiotics were detected in hospital wastewater effluent. The antibiotic-resistant pathogenic bacteria, E. coli (31 %), Staphylococcus sp. (23%), Acinetobactersp. (19%), Streptococcus sp. (10%), Pseudomonas aerugenosa (8%), Klebsiella pneumonia (6%), Moraxella sp. (3%), Aeromonas hydrophila (1%) and Streptomycessp. (1%) were found as indigenous bacteria which indicate increase the risk of ARGs which directly effect on public health. The study's findings made apparent the significance of developing a specific treatment plant at the site to remove antibiotics before discharging hospital wastewater to the natural environment. Therefore, it is necessary to develop and apply innovative treatment technologies as a national policy to protect people from silent killers who will no longer be susceptible to antibiotics in the future, which has been identified as a challenge in the health sector.
Towards Image - Based Personalization of Glioblastoma Therapy A Clinical and Biological Validation Study of a Novel, Deep Learning - Driven Tumor Growth Model
Authors: Metz Marie-Christin , Ezhov Ivan , Zimmer Lucas , Peeken Jan C. , Buchner Josef A. , Lipkova Jana , Kofler Florian , Waldmannstetter Diana , Delbridge Claire , Diehl Christian , Bernhardt Denise , Schmidt-Graf Friederike , Gempt Jens , Combs Stephanie E. , Zimmer Claus , Menze Bjoern , Wiestler Benedikt
Published: January 2023

Background
The diffuse growth pattern of glioblastoma is one of the main challenges for improving patient survival. Computational tumor growth modeling has emerged as a promising tool to guide personalized therapy. Here, we performed clinical and biological validation of a novel, deep learning - based growth model, aiming to close the gap between the experimental state and clinical implementation.
Methods
124 patients from The Cancer Genome Archive network and 397 patients from the UCSF Glioma MRI Dataset were assessed for correlations between clinical data, genetic pathway activation maps (generated with PARADIGM; TCGA only), and infiltration (Dw) as well as proliferation (r) parameters stemming from a Fisher-Kolmogorov growth model adjusted to the patients’ preoperative images using deep learning. Cox multivariable regression and Spearman correlation were performed to test for statistical significance. To further evaluate clinical potential, we performed the same growth modeling on preoperative MRI data from 30 patients of our institution and compared model-derived tumor volume and recurrence coverage with standard radiotherapy plans.
Results
The parameter ratio Dw/r (p < 0.05 in TCGA) as well as the simulated tumor volume (p < 0.05 in TCGA and UCSF) were significantly inversely correlated with overall survival. Interestingly, we observed a significant correlation between 11 signaling pathways that are associated with proliferation, and the estimated proliferation parameter r. Depending on the cutoff value for tumor cell density, we observed a significant improvement of recurrence coverage without significantly increased radiation volume utilizing model-derived target volumes instead of standard radiation plans.
Conclusion
Identifying a significant correlation between computed growth parameters, and clinical and biological data, we highlight the potential of tumor growth modeling for individualized therapy of glioblastoma. This might improve accuracy of personalized radiation planning in the near future.
Diagnostic Value of D-Dimer Concentration for Pregnancy-related Venous Thromboembolism: A Retrospective Study
Authors: Zhang Chao , Liang Ruxian , Long Yan
Published: January 2023

Background
Venous thromboembolism includes deep vein thrombosis and pulmonary embolism. Pulmonary embolism is the leading cause of maternal death and results in adverse pregnancy outcomes. Plasma D-dimer detection has high diagnostic value in nonpregnant people. We retrospectively analyzed factors affecting the plasma D-dimer concentration to establish a pregnancy-specific reference interval of plasma D-dimer. Our goal was to explore the diagnostic value of D-dimer for pregnancy-related venous thromboembolism.
Methods
The general condition, complications, and pregnancy outcomes of 2,012 women hospitalized for delivery from 1 January 2020 to 31 December 2020 in Beijing Friendship Hospital Affiliated to Capital Medical University were collected. The D-dimer concentration was collected in the first, second, and third trimesters and within 48 hours after delivery. The trend of the D-dimer concentration and influencing factors were statistically analyzed. Pregnancy-specific D-dimer reference intervals were established.
Results
The plasma D-dimer concentration gradually increased as pregnancy progressed. The median (2.5th–97.5th percentiles) in the first, second, and third trimesters and within 48 hours postpartum were 0.60 (0.30–1.50), 1.15 (0.31–5.38), 1.70 (0.80–4.89), and 4.65 (1.12–40.78) µg/mL, respectively. In the first trimester, multiparity and in vitro fertilization and embryo transfer (IVF-ET) were associated with significantly higher D-dimer concentrations. In the third trimester, twin pregnancy and IVF-ET were associated with significantly higher D-dimer concentrations. Cesarean section was an independent risk factor within 48 hours postpartum. After removal of interfering factors, reference intervals of the D-dimer levels in the first trimesters were established, 95%CI (unilateral) ~ 1.00µg/mL. The optimal cutoff value for VTE in the third trimester predicted by the ROC curve was 3.40 µg/mL.
Conclusions
The plasma D-dimer level gradually increased as pregnancy progressed. Risk factors for abnormally high D-dimer levels included multiparity and IVF-ET in the first trimester, twin pregnancy and IVF-ET in the third trimester, and cesarean section in puerperium. Upper limit value of the D-dimer levels in the first and third trimesters were suggested with 1.00 µg/mL and 3.40 µg/mL.
Epigenome-wide association study of diabetic chronic kidney disease progression in the Korean population: the KNOW-CKD study
Authors: Park Sue K. , Sung Hye Youn , Lee Sangjun , Han Miyeun , An Woo Ju , Ryu Hyunjin , Kang Eunjeong , Park Yong Seek , Lee Seung Eun , Ahn Curie , Oh Kook-Hwan , Ahn Jung-Hyuck
Published: January 2023

Since the etiology of diabetic chronic kidney disease (CKD) is multifactorial, studies on DNA methylation for kidney function deterioration have rarely been performed despite the need for an epigenetic approach. Therefore, this study aimed to identify epigenetic markers associated with CKD progression based on the decline in the estimated glomerular filtration rate in diabetic CKD patients in Korea. An epigenome-wide association study (EWAS) was performed using whole blood samples from 180 CKD patients recruited from the KNOW-CKD cohort. Pyrosequencing was also performed on 133 CKD participants as an external replication analysis. Functional analyses, including the analysis of disease-gene networks, reactome pathways, and protein-protein interaction networks, were conducted to identify the biological mechanisms of CpG sites. A phenome-wide association study was performed to determine the associations between CpG sites and other phenotypes. Two epigenetic markers, cg10297223 on AGTR1 (EWAS: ∆M-value = 0.365, false discovery rate (FDR) = 3.18E-03, pyrosequencing: Beta (SE) = 0.788 (0.397), P-value = 4.90E-02) and cg02990553 on KRT28 (EWAS: ∆M-value = 0.350, FDR = 2.84E-04, pyrosequencing: Beta (SE) = 0.459 (0.912), P-value = 6.10E-01), were found to be associated with diabetic CKD progression. Based on the functional analyses, other phenotypes (blood pressure and cardiac arrhythmia for AGTR1) and biological pathways (keratinization and cornified envelope for KRT28) related to CKD were also identified. cg10297223 on AGTR1 and cg02990553 on KRT28 are associated with diabetic CKD progression in the Korean population. Additional studies on the association between whole blood- and kidney tissue-specific DNA methylation are needed.
Micropropagation studies in Bergenia ligulata: A potential plant remedy to kidney stones
Authors: DEEBA FARAH , Singh Pooja , Warsi Zafar Iqbal , Kumari Mamta , Rahman Laiq Ur
Published: January 2023

Rhizomes of Bergenia are frequently used as antiurolithiatic agents in hilly regions of India, leading to this plant becoming threatened. Furthermore, Bergenia also serves as a diuretic, antitussive, and antipyretic, and is used as a treatment for diarrhoea, vomiting, fever, cough, pulmonary infections, menorrhagia, excessive uterine haemorrhage, and stomach ulcers. Using leaf/petiole explants from Bergenia ligulata, one-step regeneration was demonstrated. MS media supplemented with 0.2 and 0.4 mg L-1 BAP with 0.1 mg L-1 NAA resulted in the highest number of shoots on either explant (leaf/petiole). However, multiple shoots were produced on MS media fortified with 2 mg L-1 BAP and 0.5 mg L-1 Kn. An efficient and rapid regeneration protocol for this plant is presented in this study.