bluuebunny/crossref_metadata_2025 huggingface dataset:
Long Noncoding RNA Linc00152 Functions as a Tumor Propellant in Pan-Cancer
Authors: Xu Shouping , Wan Lin , Yin Huizi , Xu Hongbiao , Zheng Wei , Shen Meiying , Zhang Zhongmin , Pang Da
Published: December 2017

Background/Aims: The oncogenic role of linc00152 in pan-cancer is unclear. Methods: In this study, RNA-Seq of 33 breast specimens was performed, and the expression of linc00152 was validated by qPCR using 50 paired breast cancer tissues and adjacent normal tissues. This result combined with the expression of linc00152 in pan-cancer was revalidated by Gene Expression Omnibus and The Cancer Genome Atlas data. Next, the oncogenic roles of linc00152 in view of prognosis, chemoresistance, genomic and epigenetic regulation, including DNA methylation and histone modification, potential biological function enrichment, and basic molecular function in pan-cancer, were also evaluated in vitro and in vivo. Results: Linc00152 is upregulated in pan-cancer, especially in progressive cancer, and the high expression of linc00152 may lead to a worse prognosis and chemoresistance in pan-cancer patients. Amplification, DNA hypomethylation, promoter-like lncRNA characteristics and super-enhancer regulation are the drivers that lead to the upregulation of linc00152 in pan-cancer. Meanwhile, linc00152 was involved in cancer-related pathways, infection and immune response-associated pathways by enriched analysis using TCGA data. Finally, linc00152 was confirmed to promote the proliferation, migration and invasion in MDA-MB-231, SGC-7901 and 786-O. Moreover, RIP and RNA pull-down assays indicated that linc00152 can bind to EZH2 directly. Conclusion: All of the results indicated that linc00152 acted as an oncogenic propellant from various perspectives, and it may be an effective therapy target in pan-cancer.
Amphibians from Santa Isabel do Rio Negro, Brazilian Amazonia
Authors: Menin Marcelo , Carvalho Vinicius Tadeu de , Almeida Alexandre P. , Gordo Marcelo , Oliveira Deyla P. , Luiz Luciana F. , Campos Juliana V. , Hrbek Tomas
Published: December 2017

A species list of amphibians from Santa Isabel do Rio Negro in Brazilian Amazonia is provided. Collections were made from March–April 2012 along each of two 3-km trails with the following sampling methods: (1) pitfall traps with drift fences; (2) visual and auditory surveys; and (3) chance encounters. The trail at Daraá is north of the Rio Negro, whereas the other in Ayuanã is south of the river. Forty species of anurans and one salamander species representing 20 genera and nine families were recorded. The species composition was compared with those of 16 other studies conducted in the Guiana, Imeri, and Jaú areas of endemism, where species richness varies from 21–63, and similarity indices range from 23–100%. The anuran fauna at our sites resembles that of Flota Faro in eastern Amazonia more than it does that of the nearest site in the Departamento del Guainía of Colombia. The index of similarity is extremely variable between sites of the same and distinct areas of endemism. This pattern also was observed in the cluster analysis. As expected, geographically close areas have similar faunal compositions. However, the anuran fauna of Parque Nacional do Jaú (Jaú area of endemism) resembles that of Manaus (Guiana area of endemism) more closely than it does that of the Ayuanã River, which belongs to the same area of endemism as Parque Nacional do Jaú. The limits of the areas of endemism are better defined by the presence / absence of other terrestrial vertebrates, such as birds and mammals, than by the assemblage of amphibians and squamate reptiles.
Sex Differences in Hemodynamic and Microvascular Mechanisms of Myocardial Ischemia Induced by Mental Stress
Authors: Sullivan Samaah , Hammadah Muhammad , Al Mheid Ibhar , Wilmot Kobina , Ramadan Ronnie , Alkhoder Ayman , Isakadze Nino , Shah Amit , Levantsevych Oleksiy , Pimple Pratik M. , Kutner Michael , Ward Laura , Garcia Ernest V. , Nye Jonathon , Mehta Puja K. , Lewis Tené T. , Bremner J. Douglas , Raggi Paolo , Quyyumi Arshed A. , Vaccarino Viola
Published: December 2017

Objective—

To investigate sex-specific vascular mechanisms for mental stress-induced myocardial ischemia (MSIMI).

Approach and Results—

Baseline data from a prospective cohort study of 678 patients with coronary artery disease underwent myocardial perfusion imaging before and during a public speaking stressor. The rate-pressure product response was calculated as the difference between the maximum value during the speech minus the minimum value during rest. Peripheral vasoconstriction by peripheral arterial tonometry was calculated as the ratio of pulse wave amplitude during the speech over the resting baseline; ratios <1 indicate a vasoconstrictive response. MSIMI was defined as percent of left ventricle that was ischemic and as a dichotomous variable. Men (but not women) with MSIMI had a higher rate-pressure product response than those without MSIMI (6500 versus 4800 mm Hg bpm), whereas women (but not men) with MSIMI had a significantly lower peripheral arterial tonometry ratio than those without MSIMI (0.5 versus 0.8). In adjusted linear regression, each 1000-U increase in rate-pressure product response was associated with 0.32% (95% confidence interval, 0.22–0.42) increase in inducible ischemia among men, whereas each 0.10-U decrease in peripheral arterial tonometry ratio was associated with 0.23% (95% confidence interval, 0.11–0.35) increase in inducible myocardial ischemia among women. Results were independent of conventional stress-induced myocardial ischemia.

Conclusions—

Women and men have distinct cardiovascular reactivity mechanisms for MSIMI. For women, stress-induced peripheral vasoconstriction with mental stress, and not increased hemodynamic workload, is associated with MSIMI, whereas for men, it is the opposite. Future studies should examine these pathways on long-term outcomes.
Quantification of the Arrhythmogenic Effects of Spontaneous Atrial Extrasystole Using High-Resolution Epicardial Mapping
Authors: Teuwen Christophe P. , Kik Charles , van der Does Lisette J.M.E. , Lanters Eva A.H. , Knops Paul , Mouws Elisabeth M.J.P. , Bogers Ad J.J.C. , de Groot Natasja M.S.
Published: December 2017

Background

Atrial extrasystoles (AES) can initiate atrial fibrillation. However, the impact of spontaneous AES on intra-atrial conduction is unknown. The aims of this study were to examine conduction disorders provoked by AES and to correlate these conduction differences with patient characteristics, mapping locations, and type of AES.

Methods and Results

High-resolution epicardial mapping (electrodes N=128 or N=192; interelectrode distance, 2 mm) of the entire atrial surface was performed in patients (N=164; 69.5% male; age 67.2±10.5 years) undergoing open-chest cardiac surgery. AES were classified as premature, aberrant, or prematurely aberrant. Conduction delay and conduction block were quantified during sinus rhythm and AES and subsequently compared. Median incidence of conduction delay and conduction block during sinus rhythm was 1.2% (interquartile, 0%−2.3%) and 0.4% (interquartile, 0%−2.1%). In comparison, the median incidence of conduction delay and conduction block during 339 AES was respectively 2.8% (interquartile, 1.3%−4.6%) and 2.2% (interquartile, 0.3%−5.1%) and differed between the types of AES (prematurely aberrant>aberrant>premature). The degree of prematurity was not associated with a higher incidence of conduction disorders (
P
>0.05). In contrast, a higher degree of aberrancy was associated with a higher incidence of conduction disorders; AES emerging as epicardial breakthrough provoked most conduction disorders (
P
≥0.002). AES caused most conduction disorders in patients with diabetes mellitus and left atrial dilatation (
P
<0.05).

Conclusions

Intraoperative high-resolution epicardial mapping showed that conduction disorders are mainly provoked by prematurely aberrant AES, particularly in patients with left atrial dilation and diabetes mellitus or emerging as epicardial breakthrough.
Prognostic Implications of Magnetic Resonance–Derived Quantification in Asymptomatic Patients With Organic Mitral Regurgitation
Authors: Penicka Martin , Vecera Jan , Mirica Daniela C. , Kotrc Martin , Kockova Radka , Van Camp Guy
Published: December 2017

Background:

Magnetic resonance imaging (MRI) is an accurate method for the quantitative assessment of organic mitral regurgitation (OMR). The aim of the present study was to compare the discriminative power of MRI quantification and the recommended Doppler echocardiography (ECHO)–derived integrative approach to identify asymptomatic patients with OMR and adverse outcome.

Methods:

The study population consisted of 258 asymptomatic patients (63±14 years, 60% men) with preserved left ventricular ejection fraction (>60%) and chronic moderate and severe OMR (flail 25%, prolapse 75%) defined by using the ECHO-derived integrative approach. All patients underwent MRI to quantify regurgitant volume (RV) of OMR by subtracting the aortic forward flow volume from the total left ventricular stroke volume. Severe OMR was defined as RV≥60 mL.

Results:

Mean ECHO-derived RV was on average 17.1 mL larger than the MRI-derived RV (
P
<0.05). Concordant grading of OMR severity with both techniques was observed in 197 (76%) individuals with 62 (31%) patients having severe OMR (MRI SEV-ECHO SEV) and 135 (69%) patients having moderate OMR (MRI MOD-ECHO MOD). The remaining 61 (24%) individuals had discordant findings (MRI SEV-ECHO MOD or MRI MOD-ECHO SEV) between the 2 techniques. The majority of these differences in OMR classification were observed in patients with late systolic or multiple jets (both κ<0.2). Patients with eccentric jets showed moderate agreement (κ=0.53; 95% confidence interval, 0.41–0.64). In contrast, a very good agreement (κ=0.90; 95% confidence interval, 0.82–0.98) was observed in a combination of holosystolic, central, and single jet. During a median follow-up of 5.0 years (interquartile range, 3.5–6.0 years), 38 (15%) patients died and 106 (41%) either died or developed indication for mitral valve surgery. In separate Cox regression analyses, the MRI-derived left ventricular end-systolic volume index, RV, and OMR category (severe versus moderate), and the ECHO-derived OMR category were independent predictors of all-cause mortality (all
P
<0.05). The MRI-derived RV showed the largest area under the curve to predict mortality (0.72) or its combination with the development of indication for mitral valve surgery (0.83).

Conclusions:

The findings of the present study suggest that the MRI-derived assessment of OMR can better identify patients with severe OMR and adverse outcome than ECHO-derived integrative approach warranting close follow-up and perhaps, early mitral valve surgery.
Risk Factors of Sudden Cardiac Death in the Young
Authors: Jayaraman Reshmy , Reinier Kyndaron , Nair Sandeep , Aro Aapo L. , Uy-Evanado Audrey , Rusinaru Carmen , Stecker Eric C. , Gunson Karen , Jui Jonathan , Chugh Sumeet S.
Published: December 2017

Background:

Prevention of sudden cardiac arrest (SCA) in the young remains a largely unsolved public health problem, and sports activity is an established trigger. Although the presence of standard cardiovascular risk factors in the young can link to future morbidity and mortality in adulthood, the potential contribution of these risk factors to SCA in the young has not been evaluated.

Methods:

We prospectively ascertained subjects who experienced SCA between the ages of 5 and 34 years in the Portland, Oregon, metropolitan area (2002–2015, catchment population ≈1 million). We assessed the circumstances, resuscitation outcomes, and clinical profile of subjects who had SCA by a detailed evaluation of emergency response records, lifetime clinical records, and autopsy examinations. We specifically evaluated the association of standard cardiovascular risk factors and SCA, and sports as a trigger for SCA in the young.

Results:

Of 3775 SCAs in all age groups, 186 (5%) occurred in the young (mean age 25.9±6.8, 67% male). In SCA in the young, overall prevalence of warning signs before SCA was low (29%), and 26 (14%) were associated with sports as a trigger. The remainder (n=160) occurred in other settings categorized as nonsports. Sports-related SCAs accounted for 39% of SCAs in patients aged ≤18, 13% of SCAs in patients aged 19 to 25, and 7% of SCAs in patients aged 25 to 34. Sports-related SCA cases were more likely to present with shockable rhythms, and survival from cardiac arrest was 2.5-fold higher in sports-related versus nonsports SCA (28% versus 11%;
P
=0.05). Overall, the most common SCA-related conditions were sudden arrhythmic death syndrome (31%), coronary artery disease (22%), and hypertrophic cardiomyopathy (14%). There was an unexpectedly high overall prevalence of established cardiovascular risk factors (obesity, diabetes mellitus, hypertension, hyperlipidemia, smoking) with ≥1 risk factors in 58% of SCA cases.

Conclusions:

Sports was a trigger of SCA in a minority of cases, and, in most patients, SCA occurred without warning symptoms. Standard cardiovascular risk factors were found in over half of patients, suggesting the potential role of public health approaches that screen for cardiovascular risk factors at earlier ages.
Helicity and Vorticity of Pulmonary Arterial Flow in Patients With Pulmonary Hypertension: Quantitative Analysis of Flow Formations
Authors: Schäfer Michal , Barker Alex J. , Kheyfets Vitaly , Stenmark Kurt R. , Crapo James , Yeager Michael E. , Truong Uyen , Buckner J. Kern , Fenster Brett E. , Hunter Kendall S.
Published: December 2017

Background

Qualitative and quantitative flow hemodynamic indexes have been shown to reflect right ventricular (
RV
) afterload and function in pulmonary hypertension (
PH
). We aimed to quantify flow hemodynamic formations in pulmonary arteries using 4‐dimensional flow cardiac magnetic resonance imaging and the spatial velocity derivatives helicity and vorticity in a heterogeneous PH population.

Methods and Results

Patients with PH (n=35) and controls (n=10) underwent 4‐dimensional flow magnetic resonance imaging study for computation of helicity and vorticity in the main pulmonary artery (
MPA
), the right pulmonary artery, and the
RV
outflow tract. Helicity and vorticity were correlated with standard
RV
volumetric and functional indexes along with
MPA
stiffness assessed by measuring relative area change. Patients with
PH
had a significantly decreased helicity in the
MPA
(8 versus 32 m/s
2
;
P
<0.001), the right pulmonary artery (24 versus 50 m/s
2
;
P
<0.001), and the
RV
outflow tract–
MPA
unit (15 versus 42 m/s
2
;
P
<0.001). Vorticity was significantly decreased in patients with PH only in the right pulmonary artery (26 versus 45 1/s;
P
<0.001). Total helicity computed correlated with the cardiac magnetic resonance imaging–derived ventricular‐vascular coupling (−0.927;
P
<0.000), the
RV
ejection fraction (0.865;
P
<0.0001), cardiac output (0.581;
P
<0.0001), mean pulmonary arterial pressure (−0.581;
P
=0.0008), and relative area change measured at the
MPA
(0.789;
P
<0.0001).

Conclusions

The flow hemodynamic character in patients with PH assessed via quantitative analysis is considerably different when compared with healthy and normotensive controls. A strong association between helicity in pulmonary arteries and ventricular‐vascular coupling suggests a relationship between the mechanical and flow hemodynamic domains.
Prodromus of a fern flora for Bolivia. XXVII. Pteridaceae
Authors: KESSLER MICHAEL , SMITH ALAN R. , PRADO JEFFERSON
Published: December 2017

We provide a synopsis to the Pteridaceae s.l. in Bolivia, a family comprising four subfamilies, 27 genera, and 154 species. These totals, approximating those for Dryopteridaceae and Polypodiaceae, make this one of the most genus-and species-rich families in Bolivia. New combinations are made for two species of Argyrochosma: A. flava and A. tenera. Eight species of Pteridaceae are considered endemic: Adiantum solomonii, A. squamulosum, Cheilanthes glutinosa, Eriosorus angustus, E. ascendens, E. madidiensis, Pteris boliviensis, and P. lellingeri.
Can a Simple Dynamical System Describe the Interplay between Drag and Buoyancy in Terrain-Induced Canopy Flows?
Authors: De Roo Frederik , Banerjee Tirtha
Published: December 2017

Under nonneutral stratification and in the presence of topography, the dynamics of turbulent flow within a canopy is not yet completely understood. This has, among other consequences, serious implications for the measurement of surface–atmosphere exchange by means of eddy covariance: for example, the measurement of carbon dioxide fluxes is strongly influenced if drainage flows occur during night, when the flow within the canopy decouples from the flow aloft. An improved physical understanding of the behavior of scalars under canopy turbulence in complex terrain is urgently needed. In the present work, the authors investigate the dynamics of turbulent flow within sloped canopies, focusing on the slope wind and potential temperature. The authors concentrate on the presence of oscillatory behavior in the flow variables in terms of switching of flow regimes by conducting linear stability analysis. The authors revisit and correct the simplified theory that exists in the literature, which is based on the interplay between the drag force and the buoyancy. The authors find that the simplified description of this dynamical system cannot exhibit the observed richness of the dynamics. To augment the simplified dynamical system’s analysis, the authors make use of large-eddy simulation of a three-dimensional hill covered by a homogeneous forest and analyze the phase synchronization behavior of the buoyancy and drag forces in the momentum budget to explore the turbulent dynamics in more detail.
Impact of Winter Ural Blocking on Arctic Sea Ice: Short-Time Variability
Authors: Chen Xiaodan , Luo Dehai , Feldstein Steven B. , Lee Sukyoung
Published: December 2017

Using daily reanalysis data from 1979 to 2015, this paper examines the impact of winter Ural blocking (UB) on winter Arctic sea ice concentration (SIC) change over the Barents and Kara Seas (BKS). A case study of the sea ice variability in the BKS in the 2015/16 and 2016/17 winters is first presented to establish a link between the BKS sea ice variability and UB events. Then the UB events are classified into quasi-stationary (QUB), westward-shifting (WUB), and eastward-shifting (EUB) UB types. It is found that the frequency of the QUB events increases significantly during 1999–2015, whereas the WUB events show a decreasing frequency trend during 1979–2015.

Moreover, it is shown that the variation of the BKS-SIC is related to downward infrared radiation (IR) and surface sensible and latent heat flux changes due to different zonal movements of the UB. Calculations show that the downward IR is the main driver of the BKS-SIC decline for QUB events, while the downward IR and surface sensible heat flux make comparable contributions to the BKS-SIC variation for WUB and EUB events. The SIC decline peak lags the QUB and EUB peaks by about 3 days, though QUB and EUB require lesser prior SIC. The QUB gives rise to the largest SIC decline likely because of its longer persistence, whereas the BKS-SIC decline is relatively weak for the EUB. The WUB is found to cause a SIC decline during its growth phase and an increase during its decay phase. Thus, the zonal movement of the UB has an important impact on the SIC variability in BKS.
Evaluation of Extratropical Cyclone Precipitation in the North Atlantic Basin: An Analysis of ERA-Interim, WRF, and Two CMIP5 Models
Authors: Booth James F. , Naud Catherine M. , Willison Jeff
Published: December 2017

The representation of extratropical cyclone (ETC) precipitation in general circulation models (GCMs) and the Weather Research and Forecasting (WRF) Model is analyzed. This work considers the link between ETC precipitation and dynamical strength and tests if parameterized convection affects this link for ETCs in the North Atlantic basin. Lagrangian cyclone tracks of ETCs in ERA-Interim (ERAI), GISS and GFDL CMIP5 models, and WRF with two horizontal resolutions are utilized in a compositing analysis. The 20-km-resolution WRF Model generates stronger ETCs based on surface wind speed and cyclone precipitation. The GCMs and ERAI generate similar composite means and distributions for cyclone precipitation rates, but GCMs generate weaker cyclone surface winds than ERAI. The amount of cyclone precipitation generated by the convection scheme differs significantly across the datasets, with the GISS model generating the most, followed by ERAI and then the GFDL model. The models and reanalysis generate relatively more parameterized convective precipitation when the total cyclone-averaged precipitation is smaller. This is partially due to the contribution of parameterized convective precipitation occurring more often late in the ETC’s life cycle. For reanalysis and models, precipitation increases with both cyclone moisture and surface wind speed, and this is true if the contribution from the parameterized convection scheme is larger or not. This work shows that these different models generate similar total ETC precipitation despite large differences in the parameterized convection, and these differences do not cause unexpected behavior in ETC precipitation sensitivity to cyclone moisture or surface wind speed.
Coordination of platelet agonist signaling during the hemostatic response in vivo
Authors: Shen Jian , Sampietro Sara , Wu Jie , Tang Juan , Gupta Shuchi , Matzko Chelsea N. , Tang Chaojun , Yu Ying , Brass Lawrence F. , Zhu Li , Stalker Timothy J.
Published: December 2017

Key Points

Coordinated thromboxane A2 and ADP/P2Y12 signaling is required for platelet accumulation in the outer shell region of hemostatic plugs. Platelet activation within the hemostatic plug core region is predominantly mediated by thrombin.
Dynamic Risk Management of Commodity Operations: Model and Analysis
Authors: Devalkar Sripad K. , Anupindi Ravi , Sinha Amitabh
Published: December 2017

Adverse commodity prices can cause significant negative cash flows and expose firms that deal in commodities to financial distress. In this paper we consider the dynamic risk management problem for a commodity processor operating in a partially complete market, facing both price uncertainty and significant financial distress costs. The firm procures an input commodity, processes it to produce an output commodity, and trades the output commodity in each period over a multiperiod horizon. The firm’s objective is to dynamically and jointly plan operational and financial hedging decisions to maximize the market-based value of cash flows under a time-consistent risk measure. We show that the optimal operational policy has the same price and horizon dependent threshold structure that obtains when markets are complete. We also characterize conditions under which a myopic policy is optimal. We present numerical studies to illustrate that (i) compared to using a myopic policy or a policy that neglects to model financial distress costs, the advantage of using the optimal policy is significant for firms that face moderate financial distress costs and have excess procurement capacity; (ii) more acute financial distress costs reduce the firm’s market-based value and throughput; and (iii) financial hedging provides considerable benefit by reducing the incidence of large negative cash flows.

The online appendix is available at https://doi.org/10.1287/msom.2017.0647 .
Knowledge Creation Across Worldviews: How Metaphors Impact and Orient Group Creativity
Authors: Biscaro Claudio , Comacchio Anna
Published: December 2017

Metaphors are central in organization theory for they help the creation of knowledge by altering concepts or generating new ones. Yet, despite their importance, little is known about how metaphors are used in processes of knowledge creation across worldviews. In such contexts, participants maintain their specialization, work separately, and resort to interpretable devices like metaphors to create together. With a longitudinal study of a multidisciplinary scientific project aimed at repairing broken spinal cord tissues, we show how metaphors facilitate collective knowledge creation. We contribute to the theory of knowledge creation across worldviews by showing the consequences of the diverse creative outcomes of metaphors on the orientation and stability of the collective work. Moreover, we propose how to control and predict the creative outcomes of metaphors by modifying the knowledge bases that are engaged in the creative process. We contribute to the theory on cycles of knowledge creation by showing that a metaphor can extend knowledge both horizontally and vertically, and by specifying the relationships between the knowledge created and the elements that simultaneously populate the creative cycles.
Desenvolvimento e Características Psicométricas das Facetas Adicionais da Versão em Português Europeu do WHOQOL-SRPB (Spirituality, religiousness and personal beliefs)
Authors: Costa Catré Maria Nazarete , Ferreira Joaquim Armando , Pessoa Teresa , Catré Acácio , Catré Maria Costa , Pereira Marco
Published: December 2017

O grupo WHOQOL-SRPB da Organização Mundial de Saúde (OMS) desenvolveu o WHOQOL-SRPB, um instrumento de medida que avalia o domínio da espiritualidade, religiosidade e crenças pessoais da qualidade de vida (WHOQOL-SRPB Group, 2006). A validação das versões nacionais desse instrumento de medida obedece a um protocolo definido pela OMS/Grupo WHOQOL, que envolve as seguintes etapas: (1) tradução dos instrumentos; (2) etapa qualitativa; (3) desenvolvimento das escalas de resposta; (4) estudo de campo quantitativo. Os dois estudos que compõem o presente artigo têm os seguintes objetivos: (1) apresentar os resultados da etapa qualitativa, com grupos focais, que conduziram ao desenvolvimento de duas novas facetas – SP9. Relação com os outros e SP10. Estilo de vida (Estudo 1); e (2) apresentar as características psicométricas destas duas facetas adicionais (Estudo 2). O estudo qualitativo foi composto por 12 grupos focais e o estudo de campo quantitativo abrangeu uma amostra de 606 participantes. Os resultados atestam a fiabilidade e validade das novas facetas portuguesas, constituindo‑se como um acréscimo relevante e original ao questionário WHOQOL-SRPB.
UMA ANÁLISE DO CONSUMO DE VIAGENS POR MORADORES DA COMUNIDADE DA ROCINHA À LUZ DA TAXONOMIA DE HOLT
Authors: Falcão Roberto Pessoa de Queiroz , Toda Favio Akiyoshi
Published: December 2017

Consumidores de comunidades de baixa renda, outrora discriminados, passaram a estar mais presentes nos destinos turísticos, seja pelo acesso ampliado às viagens, em função de preços mais competitivos dos transportes aéreos, ou por sua ascensão econômica. Contudo, o consumo de viagens pode ser visto como um fenômeno além de seu aspecto utilitário de locomoção, repouso e relaxamento. Ele se manifesta como sendo recheado de aspectos simbólicos, que não raro, são influenciadores na decisão de compra. Este trabalho teve objetivo investigar significados do consumo de viagens entre clientes, residentes da comunidade da Rocinha, Brasil. As metáforas de Holt foram adotadas como arcabouço teórico e parâmetro de análise dos significados observados nas falas dos entrevistados. Tais metáforas representam classificações dos significados do consumo vistos sob as perspectivas de possível interação entre pessoas mediada pelo serviço. A coleta de dados foi realizada através de 12 entrevistas em profundidade com clientes, seguida de triangulação com dois agentes de viagem da Rocinha. Foi realizada uma codificação das entrevistas e posterior análise do conteúdo pelo protocolo de análise de conteúdo de Bardin. Trata-se de um estudo qualitativo, de abordagem interpretativista, que não busca realizar generalizações.
TURISMO GASTRONÔMICO: UMA PESQUISA BIBLIOMÉTRICA EM BASES DE DADOS NACIONAIS E INTERNACIONAIS
Authors: Ribeiro-Martin Clarissa de Souza , Silveira-Martins Elvis
Published: December 2017

Esta pesquisa objetiva realizar um levantamento bibliométrico sobre o construto turismo gastronômico em bases de dados nacionais e internacionais. Foram analisados 45 artigos identificados nas bases de dados periódicos CAPES, Spell, Proquest, Ebsco. Realizaram-se diferentes análises como: evolução temporal, identificação dos periódicos, cálculo da lei de Bradford, tipos de pesquisas realizadas, centralidade de Freeman, redes de autores, mensuração do poder de Bonacich, comparativo com a Lei de Lotka, redes de universidades, trabalhos com maior número de referências, principais palavras dos objetivos. Verificou-se, entre outros aspectos, que o core do assunto são os periódicos: Casos, Rosa dos Ventos, além das revistas Estudios y Perspectivas en Turismo, Tourism & Management e TuryDes. Ainda, o autor mais referenciado é Michael Hall, no entanto o trabalho com maior número de referências é de Erik Cohen. O pesquisador Emilio Morales-Fernández e as universidades de Aveiro e Loyola Andalucía realizam o maior número de parcerias científicas.
TURISMO DE AVENTURA EM TRÊS COROAS: UMA ANÁLISE DA SUSTENTABILIDADE A PARTIR DOS CRITÉRIOS DO ADVENTURE TOURISM DEVELOPMENT INDEX
Authors: Decol Felipe , Lanzer Rosane Maria
Published: December 2017

O turismo de aventura, visto como forma de turismo alternativo, deve seguir os princípios da sustentabilidade. A pesquisa foi realizada em Três Coroas, destino de turismo de aventura, caracterizado principalmente pelo rafting. O objetivo deste trabalho foi avaliar a sustentabilidade das atividades do turismo de aventura em Três Coroas utilizando critérios do Adventure Tourism Development Index (ATDI). Para realização do estudo, utilizou-se de pesquisa de abordagem descritiva, de caráter qualitativo e quantitativo. Os dados da pesquisa foram coletados por meio de entrevistas, observação participante e pesquisas bibliográficas e utilizados para a avaliação e classificação do turismo de aventura do município, de acordo com os critérios do ATDI. Foi avaliada como positiva a relação entre o poder público e os empresários, os quais colocam o turismo como fator estratégico para o desenvolvimento do município, as boas condições da qualidade dos serviços e da infraestrutura. As deficiências foram encontradas na falta de controle sobre a capacidade de carga suportada pelo ambiente, problemas com a poluição do Rio Paranhana e a carência de serviços básicos para a população. A implementação de atividades inovadoras e atração de nichos menores poderiam valorizar o produto turístico sem a necessidade de aumento da demanda e infraestrutura.
Transformative Learning and Education for Sustainable Development
Authors: Balsiger Jörg , Förster Ruth , Mader Clemens , Nagel Ueli , Sironi Helene , Wilhelm Sandra , Zimmermann Anne B
Published: December 2017

Is higher education capable of promoting learning for change? Can transformative learning nurture spaces for innovation in education for sustainable development? A call to action from saguf.
Reduzierter Fleischkonsum als Vehikel für die große Transformation: Voraussetzungen und Realisierungschancen
Authors: Stoll-Kleemann Susanne , Johanna Schmidt Uta
Published: December 2017

Reduzierter Fleischkonsum in Industrieländern gilt als Kernstück einer großen gesellschaftlichen Transformation. Voraussetzungen dafür und Realisierungschancen haben Mitglieder der Deutschen Gesellschaft für Humanökologie im Rahmen einer sozialwissenschaftlichen
Metastudie erforscht.
Examining of the relationship between math anxiety and math achievement of elementary school students
İlkokul öğrencilerinin matematik kaygısı ile matematik başarıları arasındaki ilişkinin incelenmesi
Authors: Mutlu Yılmaz , Söylemez İhsan , Yasul Ali Fuad
Published: December 2017

This study aims to investigate relation between math anxiety and mathmatical achievement of 3hd and 4th grade  elementary school students. The participants of the study were constituted of totally 474 students, while 288 of those were attending 3hd grade, other 186 attending 4th grade. They were used math anxiety scale (13 items) developed by Mutlu & Söylemez (2017) for 3hd and 4th grades and math achievement tests for 3hd (16 items) and 4th (24 items) grades developed by Fidan (2013) as data gathering tools. They were also used pearson product-moment correlation, linear regression, t-test, oneway anova and two-step clustering methods to analyse data. The data concerning math anxiety and achievement were analysed through pearson product-moment correlation and linear regression. The relation level was found -547 between students’ math anxiety and math achievement. Besides, it was located that math anxiety explains 35% total varience of math achievement. With all that, it was used two-step clustering analyse method over math anxiety and achievement. There were found significant differences between participants’ math anxiety and math achievement scores.Extended English abstract is in the end of Full Text PDF (TURKISH) file.ÖzetBu çalışma, ilkokul 3. ve 4. sınıf öğrencilerinin matematik kaygısı ile matematik başarısı arasındaki ilişkiyi incelemeyi amaçlamaktadır. Çalışmanın katılımcılarının 288’i 3. sınıf, 186’sı 4. sınıf olmak üzere toplamda 474 öğrenciden oluşmaktadır. Çalışmada veri toplamak üzere ilkokul 3. ve 4. sınıflar için Mutlu ve Söylemez (2017) tarafından geliştirilmiş olan 13 maddelik bir kaygı ölçeği ile 3. sınıflar için 16 maddeden oluşan, 4. Sınıflar için ise 24 maddeden oluşan Fidan (2013) tarafından geliştirilen matematik başarı testleri kullanılmıştır. Verilerin analizinde Pearson Momentler Çarpımı Korelasyonu, doğrusal regresyon, t-testi ve iki adımlı kümeleme analizi yapılmıştır. Öğrencilerin matematik kaygısı ve matematik başarısı puanları arasında ilişki düzeyi -.547 olarak bulunmuştur. Ayrıca matematik kaygısının matematik başarısına ilişkin toplam varyansın %35’ini açıkladığı tespit edilmiştir. Bununla beraber matematik kaygısı ve matematik başarısı ilişkisini incelemek üzere iki adımlı kümeleme analizi yapılmıştır. Katılımcıların matematik kaygı ve matematik başarı puanları üzerinde yapılan kümeleleme analizi ile gruplar belirlenmiş, grupların matematik başarıları ve matematik kaygıları arasında anlamlı farklılıklar olduğu tespit edilmiştir.
A new ELISA for autoantibodies to steroid 21-hydroxylase
Authors: del Pilar Larosa Maria , Chen Shu , Steinmaus Nora , Macrae Hannah , Guo Liang , Masiero Stefano , Garelli Silvia , Costa Miriam Dalla , Bossowski Artur , Furmaniak Jadwiga , Betterle Corrado , Smith Bernard Rees
Published: December 2017

Background:

A new ELISA for autoantibodies to steroid 21-hydroxylase (21-OH Ab) is described.

Methods:

In the assay test sample autoantibodies form a bridge between 21-OH coated onto the plate well and liquid phase 21-OH-biotin. Bound 21-OH-biotin is detected by the addition of streptavidin peroxidase and colorogenic peroxidase substrate.

Results:

Of 100 samples from patients with autoimmune Addison’s disease, 86 (86%) were positive for 21-OH Ab ELISA whereas 84 (84%) were positive in an immunoprecipitation assay based on 125I-labeled 21-OH. Six (0.6%) of 928 healthy adult blood donors and 1 (2.0%) of 49 adult patients with type 1 diabetes mellitus (T1DM) were positive by ELISA. No samples from adult patients with Graves’ disease (GD; n=50), celiac disease (n=29), systemic lupus erythematosis (n=9) or rheumatoid arthritis (n=20) were positive by ELISA. However, 2/51 (3.9%) children with GD, 3/69 (4.3%) children with Hashimoto’s thyroiditis (HT) and 3/119 (2.5%) children with T1DM alone or associated with autoimmune thyroid disorders were ELISA positive.

Conclusions:

The new assay should be useful for screening patients known to be at increased risk of developing clinical autoimmune Addison’s disease, in particular children with HT, GD and/or T1DM.
Early onset preeclampsia is associated with an elevated mean platelet volume (MPV) and a greater rise in MPV from time of booking compared with pregnant controls: results of the CAPE study
Authors: Monteith Cathy , Egan Karl , O’Connor Hugh , Maguire Patricia , Kevane Barry , Szklanna Paulina B. , Cooley Sharon , Malone Fergal , Áinle Fionnuala Ní
Published: December 2017

Objective:

To characterise Mean platelet volume (MPV) in patients with early onset preeclampsia (EOPE) and unaffected controls from time of first antenatal visit until the postpartum.

Materials and methods:

Retrospective secondary analysis of an observational study in an Irish tertiary referral centre with 9000 deliveries annually. The MPV of 27 women with EOPE was compared to 19 unaffected controls. The inclusion criteria for the disease state was the development of EOPE defined by the National Institute for Health and Care Excellence (NICE) guideline, as new onset hypertension presenting after 20 weeks and prior to 34 weeks with significant proteinuria. Between October 2013 and July 2015 we recruited 27 women with EOPE and 19 pregnant controls. Statistical analysis was performed using paired T-test of Mann-Whitney test where appropriate and a P-value <0.05 was deemed significant.

Results:

At time of diagnosis and late in the third trimester MPV was significantly increased to 9.0 (±0.3) fL in cases of EOPE in comparison to 8.5 (±0.6) fL in normotensive controls (P<0.05). There was no significant difference during the first trimester or postpartum when comparing the MPV in EOPE to controls.

Conclusion:

Despite an increased MPV at time of diagnosis of EOPE this study did not demonstrate a potential use for increased MPV as a first trimester screening tool.
Watch out for congenital Zika syndrome in non-endemic regions
Authors: Zare Mehrjardi Mohammad
Published: December 2017

Zika virus (ZIKV), first discovered in 1947, is the most recent member of the TORCH family. It usually causes an asymptomatic or mildly symptomatic disease in infected adults but can lead to severe brain abnormalities in fetuses who are infected in utero by vertical transmission of the virus through the placenta. The constellation of these fetal/neonatal abnormalities is named as congenital Zika syndrome (CZS). Although horizontal transmission of ZIKV is largely dependent on the presence of the vector (i.e. Aedes mosquitoes), women residing in non-endemic areas are still at risk of acquiring the infection once they travel to an endemic region or have unprotected sexual contact with an infected male. Therefore, it is important for physicians practicing in non-endemic regions to be familiar with the clinical and neuroimaging manifestations of CZS and to consider this diagnosis as a potential etiology for congenital microcephaly and other fetal central nervous system abnormalities.
Dental anomalies inCaluromys(Marsupialia, Didelphimorphia, Didelphidae, Caluromyinae) and a reassessment of malformations in New World marsupials (Didelphimorphia, Microbiotheria and Paucituberculata)
Authors: Martin Gabriel M. , Chemisquy María Amelia
Published: December 2017

Dental anomalies have been documented in almost all mammalian orders, and include supernumerary or missing teeth, teeth with aberrant occlusal surfaces and/or roots and teeth in unusual positions. Our objectives were the description and categorization of dental anomalies in all species of the genusCaluromys. We studied 462 crania, recorded and classified dental anomalies in four categories: variations in occlusal/root morphology; teeth in unusual positions; supernumerary/missing teeth; presence of unshed deciduous premolars. We found anomalies in all species, with a percentage ranging from 11% to 6.3%.Caluromys derbianusproduced anomalous M4/m4 and flipped crowns, the other two species produced higher numbers of missing teeth. We infer that flipped crowns might have consequences in mastication, while other anomalies seem to be less functionally important, especially those at the end of the toothrow. Comparisons with other New World marsupials show caluromyines have more anomalies in M4/m4 shape (similar to microbiotherids) and flipped crowns, while didelphids have more supernumerary teeth, and caenolestids have more missing teeth.
Applications of stem cell-derived exosomes in tissue engineering and neurological diseases
Authors: Sun Baichuan , Peng Jiang , Wang Shoufeng , Liu Xuejian , Zhang Kaihong , Zhang Zengzeng , Wang Chong , Jing Xiaoguang , Zhou Chengfu , Wang Yu
Published: December 2017

Exosomes are extracellular vesicles with diameters of 30–100 nm that are key for intercellular communication. Almost all types of cell, including dendritic cells, T cells, mast cells, epithelial cells, neuronal cells, adipocytes, mesenchymal stem cells, and platelets, can release exosomes. Exosomes are present in human body fluids, such as urine, amniotic fluid, malignant ascites, synovial fluid, breast milk, cerebrospinal fluid, semen, saliva, and blood. Exosomes have biological functions in immune response, antigen presentation, intercellular communication, and RNA and protein transfer. This review provides a brief overview of the origin, morphological characteristics, enrichment and identification methods, biological functions, and applications in tissue engineering and neurological diseases of exosomes.
Shroomroot: An Action-Based Digital Game to Enhance Postsecondary Teaching and Learning about Mycorrhizae
Authors: Maddison Julia Amerongen , Kržić Maja , Simard Suzanne , Adderly Christopher , Khan Samia
Published: December 2017

The majority of terrestrial plants associate with fungi in symbiotic resource-exchange relationships called mycorrhizae. Because of the importance of these mycorrhizal systems to ecosystem functioning, it is crucial that future resource managers and scientists have a solid understanding of mycorrhizal ecology. Limited interest of postsecondary students in plants and fungi compared with animals, combined with difficulties visualizing below-ground processes, present challenges for learning mycorrhizal concepts. To address this, we created the digital, plant-ecology-centric, action-based game Shroomroot for use in a second year, postsecondary Introduction to Soil Science course. We then assessed effects of Shroomroot on students’ knowledge acquisition and engagement with the topic of mycorrhizal ecology using a pre- and post-test evaluation. Students’ knowledge of mycorrhizal ecology increased significantly after playing Shroomroot, and tended to increase more for items related to Shroomroot gameplay than in rewards-based game content. Student engagement with mycorrhizal content tended to increase after gameplay. These results suggest positive potential for action-based, plant-ecology-oriented digital games in a postsecondary science curriculum. Furthermore, greater understanding of mycorrhizae has the potential to improve our multifaceted relationships with the ecosystems upon which we depend.
A Novel Sterol-Signaling Pathway Governs Azole Antifungal Drug Resistance and Hypoxic Gene Repression in Saccharomyces cerevisiae
Authors: Serratore Nina D , Baker Kortany M , Macadlo Lauren A , Gress Abigail R , Powers Brendan L , Atallah Nadia , Westerhouse Kirsten M , Hall Mark C , Weake Vikki M , Briggs Scott D
Published: December 2017

During antifungal drug treatment and hypoxia, genetic and epigenetic changes occur to maintain sterol homeostasis and cellular function. In this study, we show that SET domain-containing epigenetic factors govern drug efficacy to the medically relevant azole class of antifungal drugs. Upon this discovery, we determined that Set4 is induced when Saccharomyces cerevisiae are treated with azole drugs or grown under hypoxic conditions; two conditions that deplete cellular ergosterol and increase sterol precursors. Interestingly, Set4 induction is controlled by the sterol-sensing transcription factors, Upc2 and Ecm22. To determine the role of Set4 on gene expression under hypoxic conditions, we performed RNA-sequencing analysis and showed that Set4 is required for global changes in gene expression. Specifically, loss of Set4 led to an upregulation of nearly all ergosterol genes, including ERG11 and ERG3, suggesting that Set4 functions in gene repression. Furthermore, mass spectrometry analysis revealed that Set4 interacts with the hypoxic-specific transcriptional repressor, Hap1, where this interaction is necessary for Set4 recruitment to ergosterol gene promoters under hypoxia. Finally, an erg3Δ strain, which produces precursor sterols but lacks ergosterol, expresses Set4 under untreated aerobic conditions. Together, our data suggest that sterol precursors are needed for Set4 induction through an Upc2-mediated mechanism. Overall, this new sterol-signaling pathway governs azole antifungal drug resistance and mediates repression of sterol genes under hypoxic conditions.
Habilidades cognitivas y funciones neuropsicológicas ejecutivas en niños bilingües (lengua nativa- castellano) y monolingües castellano
Authors: Canales Gabriel Ricardo , Velarde Consoli Esther , Ramírez Mendoza Juan , Lingán Huamán Susana
Published: December 2017

El estudio buscó establecer si existen diferencias en los niveles de desarrollo de habilidades cognitivas y funciones ejecutivas en niños bilingües y niños monolingües castellano, pertenecientes al mismo nivel socio- económico. Se examinó a 20 alumnos de 5° y 6° grado de la Comunidad de Socos; 24 alumnos de 5° y 6° grado de la Institución educativa “Virgen del Carmen”, Pilacucho, Huamanga; 24 estudiantes de la I.E. intercultural bilingüe: “Comunidad Shipiba” en Cantagallo, Lima; y 18 estudiantes de 5° y 6° grado de “Santa Rosa de América” del Callao; con edades que fluctúan entre los 10 y 12 años. Se examinó inteligencia con el Test de Raven y funciones neuropsicológicas ejecutivas con algunos sub tests del BANFE (Flores-Ostrosky, 2012). Los resultados mostraron diferencias significativas entre los grupos, tanto en funcionamiento cognitivo como en las funciones ejecutivas.Los alumnos que rindieron más bajo en ambas variables, fueron los niños de Socos, Ayacucho, zona rural y quechua-hablante. Luego se ubicaron los alumnos de Huamanga, capital del departamento de Ayacucho. Los que rindieron mejor fueron los alumnos del Callao y los niños de la comunidad shipiba de Cantagallo- Lima, con influencia de otro tipo de bilinguismo. Se aprecia que los factores disortogénicos: pobreza extrema, marginación social, el problema del bilinguismo quechua- castellano, estarían relacionados con el desarrollo cognitivo y de funciones ejecutivas en los niños. En conclusión se encontró diferencias significativas entre los grupos de estudiantes de diferentes realidades socio- culturales a nivel de funciones neuropsicológicas ejecutivas y en habilidades cognitivas.
Acoustic correlates of female speech under stress based on /i/-vowel measurements
Authors: Tavi Lauri
Published: December 2017

The purpose of this article is to measure and evaluate commonly identified, yet rather inconsistent, acoustic correlates of speech under stress from authentic emergency call recordings. In this study, ten different acoustic parameters are measured from manually segmented /i/-vowels and hypotheses based on previous studies are statistically tested for a set of female emergency call recordings. The statistical analyses confirm that in comparison to the neutral speech group, the speech under stress group differs in fundamental frequency, shimmer, harmonicity, Hammarberg index, F1, F2, F3 and formant dispersion, which mostly supports the findings from previous studies. Conversely, jitter and vowel duration do not show any statistical difference between the speech under stress group and the neutral group. Furthermore, the results substantiate that stress recognition using different acoustic parameters is feasible from data sets as small as vowel segments; however, the effect of inter-speaker variation must not be underestimated. In future research, a stress detection model for telephone bandpass limited speech based on the optimal combination of acoustic parameters will be created.