bluuebunny/crossref_metadata_2025 huggingface dataset:
Precision Medicine in Pediatric Cancer: Current Applications and Future Prospects
Authors: Ahmed Atif A. , Vundamati Divya S. , Farooqi Midhat S. , Guest Erin
Published: December 2018

Precision oncologic medicine is an emerging approach for cancer treatment that has recently taken giant steps in solid clinical practice. Recent advances in molecular diagnostics that can analyze the individual tumor’s variability in genes have provided greater understanding and additional strategies to treat cancers. Although tumors can be tested by several molecular methods, the use of next-generation sequencing (NGS) has greatly facilitated our understanding of pediatric cancer and identified additional therapeutic opportunities. Pediatric tumors have a different genetic make-up, with a fewer number of actionable targets than adult tumors. Nevertheless, precision oncology in the pediatric population has greatly improved the survival of patients with leukemia and solid tumors. This review discusses the current status of pediatric precision oncology and the different clinical scenarios in which it can be effectively applied.
Impact of Hydrous Manganese and Ferric Oxides on the Behavior of Aqueous Rare Earth Elements (REE): Evidence from a Modeling Approach and Implication for the Sink of REE
Authors: Liu Haiyan , Pourret Olivier , Guo Huaming , Martinez Raul E. , Zouhri Lahcen
Published: December 2018

In this study, models were used for the first time to investigate the fate and transport of rare earth elements (REE) in the presence of hydrous manganese and ferric oxides in groundwaters from the coastal Bohai Bay (China). Results showed that REE sorption is strongly dependent on pH, as well as hydrous manganese and ferric oxide content. Higher proportions of REE were sorbed by hydrous manganese oxide as compared to hydrous ferric oxides, for example in the presence of neodymium. In this case, a mean 28% of this element was sorbed by hydrous manganese oxide, whereas an average 7% sorption was observed with hydrous ferric oxides. A contrasting REE sorption behavior was observed with hydrous manganese and ferric oxide for all investigated groundwaters. Specifically, REE bound to hydrous manganese oxides showed decreasing sorption patterns with increasing atomic number. The opposite trend was observed in the presence of hydrous ferric oxides. In addition, these results suggested that light REE (from La to Sm) rather than heavy REE (from Eu to Lu) are preferentially scavenged by hydrous manganese oxide. However, the heavy REE showed a greater affinity for hydrous ferric oxides compared to light REE. Therefore, both hydrous manganese and ferric oxide are important scavengers of REE. This study shows the implication of hydrous manganese and ferric oxide sorption for the sink of REE in groundwater.
Defense Mechanisms of Two Pioneer Submerged Plants during Their Optimal Performance Period in the Bioaccumulation of Lead: A Comparative Study
Authors: Li Dian , Zhang Linglei , Chen Min , He Xiaojia , Li Jia , An Ruidong
Published: December 2018

Ceratophyllum demersum L. and Hydrilla verticillata (L.f.) Royle, two pioneer, submerged plants, effectively remove heavy metals from contaminated water. The present work evaluates the bioaccumulation and defense mechanisms of these plants in the accumulation of lead from contaminated water during their optimal performance period. C. demersum and H. verticillata were investigated after 14 days of exposure to various lead concentrations (5–80 μM). The lead accumulation in both C. demersum and H. verticillata increased with an increasing lead concentration, reaching maximum values of 2462.7 and 1792 mg kg−1 dw, respectively, at 80 μM. The biomass and protein content decreased significantly in C. demersum when exposed to lead. The biomass of H. verticillata exposed to lead had no significant difference from that of the controls, and the protein content increased for the 5–10 μM exposure groups. The malondialdehyde (MDA) content and superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO) activities were much higher in C. demersum, suggesting considerable damage from lipid peroxidation and sensitivity to lead stress. Enzyme inhibition and inactivation were also observed in C. demersum at high lead concentrations (40–80 μM). The excellent growth status, low damage from lipid peroxidation, and high activity of catalase (CAT) and phenylalanine ammonia-lyase (PAL) observed in H. verticillata illustrate its better tolerance under the same lead stress.
Role of Social and Informational Support while Deciding on Pregnancy Termination for Medical Reasons
Authors: Zaręba Kornelia , Makara-Studzińska Marta , Ciebiera Michał , Gierus Jacek , Jakiel Grzegorz
Published: December 2018

Background: Poland is a country with restrictive laws concerning abortion, which is only allowed if the mother’s life and health are in danger, in case of rape, and severe defects in the fetus. This paper specifies the forms of support expected by women considering termination from their family, people in their surroundings and professional medical personnel. Methods: Between June 2014 and May 2016 patients eligible to terminate a pregnancy for medical reasons were asked to complete an anonymous survey consisting of sixty questions to determine patient profile and forms of support expected from the society, family and professional medical personnel as well as to assess informational support provided. Results: Women do not take into consideration society’s opinion on pregnancy termination (95%). The majority of the respondents think that financial support from the state is not sufficient to provide for sick children (81%). Despite claiming to have a medium standard of life (75%), nearly half of the respondents (45%) say that they do not have the financial resources to take care of a sick child. The women have informed their partner (97%) and closest family members (82%) and a low percentage have informed friends (32%). Nearly one third (31%) have not talked to the attending gynecologist about their decision. Conclusions: The decision to terminate a pregnancy is made by mature women with a stable life situation—supported by their partner and close family. They do not expect systemic support, as they believe it is marginal, and only seek emotional support from their closest family. They appreciate support provided by professional medical personnel if it is personal.
How Outdoor Trees Affect Indoor Particulate Matter Dispersion: CFD Simulations in a Naturally Ventilated Auditorium
Authors: Hong Bo , Qin Hongqiao , Jiang Runsheng , Xu Min , Niu Jiaqi
Published: December 2018

This study used computational fluid dynamics (CFD) models, coupling with a standard k-ε model based on the Reynolds-averaged Navier-Stokes (RANS) approach and a revised generalized drift flux model, to investigate effects of outdoor trees on indoor PM1.0, PM2.5, and PM10 dispersion in a naturally ventilated auditorium. Crown volume coverage (CVC) was introduced to quantify outdoor trees. Simulations were performed on various CVCs, oncoming wind velocities and window opening sizes (wall porosities were 3.5 and 7.0%, respectively, for half and fully opened windows). The results were as follows: (1) A vortex formed inside the auditorium in the baseline scenario, and the airflow recirculation created a well-mixed zone with little variation in particle concentrations. There was a noticeable decrease in indoor PM10 with the increasing distance from the inlet boundary due to turbulent diffusion. (2) Assuming that pollution sources were diluted through the inlet, average indoor particle concentrations rose exponentially with increasing oncoming wind speed. PM10 changed most significantly due to turbulent diffusion and surface deposition reduction intensified by the increased wind velocity. (3) Increasing the window opening improved indoor cross-ventilation, thus reducing indoor particle concentrations. (4) When 2.87 m3/m2 ≤ CVC ≤ 4.73 m3/m2, indoor PM2.5 could meet requirements of the World Health Organization’s air quality guidelines (IT-3) for 24-hour mean concentrations; and (5) average indoor particle concentrations had positive correlations with natural ventilation rates (R2 = 0.9085, 0.961, 0.9683 for PM1.0, PM2.5, and PM10, respectively, when the wall porosity was 3.5%; R2 = 0.9158, 0.9734, 0.976 for PM1.0, PM2.5, and PM10, respectively, when the wall porosity was 7.0%).
Acquiring Knowledge about the Use of a Newly Developed Electronic Fetal Heart Rate Monitor: A Qualitative Study Among Birth Attendants in Tanzania
Authors: Rivenes Lafontan Sara , Sundby Johanne , Kidanto Hussein L. , Mbekenga Columba K. , Ersdal Hege L.
Published: December 2018

In an effort to reduce newborn mortality, a newly developed strap-on electronic fetal heart rate monitor was introduced at several health facilities in Tanzania in 2015. Training sessions were organized to teach staff how to use the device in clinical settings. This study explores skilled birth attendants’ perceptions and experiences acquiring and transferring knowledge about the use of the monitor, also called Moyo. Knowledge about this learning process is crucial to further improve training programs and ensure correct, long-term use. Five Focus group discussions (FGDs) were carried out with doctors and nurse-midwives, who were using the monitor in the labor ward at two health facilities in Tanzania. The FGDs were analyzed using qualitative content analysis. The study revealed that the participants experienced the training about the device as useful but inadequate. Due to high turnover, a frequently mentioned challenge was that many of the birth attendants who were responsible for training others, were no longer working in the labor ward. Many participants expressed a need for refresher trainings, more practical exercises and more theory on labor management. The study highlights the need for frequent trainings sessions over time with focus on increasing overall knowledge in labor management to ensure correct use of the monitor over time.
Development and Validation of ICT Self-Efficacy Scale: Exploring the Relationship with Cyberbullying and Victimization
Authors: Musharraf Sadia , Bauman Sheri , Anis-ul-Haque Muhammad , Malik Jamil Ahmad
Published: December 2018

The purpose of this study was to develop and validate the ICT Self-Efficacy Scale and the association of cyberbullying and victimization with ICT self-efficacy. Sample 1 (436 university students) was used to identify the factor structure of the Scale, and sample 2 (1115 university students) provided the data to confirm the factor structure (CFA), and to compute the internal consistency reliability, and convergent validity of the scale. Findings demonstrate that the new scale is a reliable and valid domain-specific measure to assess ICT Self-Efficacy for university students. Suggestions for further research with the scale are provided.
Job Burnout, Work-Family Conflict and Project Performance for Construction Professionals: The Moderating Role of Organizational Support
Authors: Wu Guangdong , Wu Yue , Li Hongyang , Dan Chenglong
Published: December 2018

Construction professionals are prone to work-family conflict and job burnout, which in turn can affect project performance during implementation of delivery. To cope with this, a questionnaire survey was undertaken with construction professionals in the Chinese construction industry and 373 valid responses were received. A theoretical model introducing organizational support as a moderating variable was developed and tested with structural equation modeling. The results showed that there is a positive correlation between work-family conflict and job burnout for construction professionals. Involving organizational support can alleviate the impact of work-family conflict on professionals’ job burnout. Meanwhile, both work-family conflict and job burnout have negative effects on project performance. Therefore, an atmosphere and culture of humanized management should be established within the construction enterprises. Additionally, organizational incentives such as regulations and rules should be formulated assisting employees achieve work-family balance. Such incentives could contribute to the construction industry and improve project performance. Furthermore, this study provides a new theoretical perspective for the management of job burnout and work-family conflict in the construction industry, complementing the existing body of knowledge.
Fair but Risky? Recycle Pricing Strategies in Closed-Loop Supply Chains
Authors: He Jianhong , Zhang Lei , Fu Xiao , Tsai Fu-Sheng
Published: December 2018

We argue that a Nash bargaining model with behavioral factors (i.e., fairness concern and risk aversion) should be introduced to the price strategizing process in the context of a closed-loop supply chain. We consider three different pricing models: The first is when both the manufacturer and the retailer have fairness concerns; the second is when both the manufacturer and the retailer have risk aversion; and the final is when the manufacturer has risk aversion but the retailer has both risk aversion and fairness concern. Then we examine the model with game theory. The results have shown that fairness and risk aversion change the optimal pricing strategy, which affects the expected profits of retailers and manufacturers. The impacts of two (relatively irrational) behavioral factors on the wholesale and retail prices of new products, the recycle price and recycle transfer price of the waste products, are not the same. For new products, the wholesale price is most affected by behavioral factors and the sales price scores second. For waste recycling products, the transfer price is most affected by behavioral factors and the recycle price scores second. When considering fairness and risk aversion in retail, fairness concern is good for both manufacturers and retailers. This innovative pricing strategy model adds implications for sustainability in supply chain operations.
Tradeoff Analysis between Spectral and Energy Efficiency Based on Sub-Channel Activity Index in Wireless Cognitive Radio Networks
Authors: Semba Yawada Prince , Trung Dong Mai
Published: December 2018

In recent years, there has been a rapid evolution of wireless technologies that has led to the challenge of high demand for spectral resources. To overcome this challenge, good spectrum management is required that calls for more efficient use of the spectrum. In this paper, we present a general system, which makes a tradeoff between the spectral efficiency (SE) and energy efficiency (EE) in the cellular cognitive radio networks (CCRN) with their respective limits. We have analyzed the system taking into account the different types of power used in the CCRN, namely the spectrum detection power (Zs) and the relay power (Zr). Optimal policy for emission power allocation formulated in the function of sub-channel activity index (SAI) as an optimization problem in order to maximize spectrum utilization and minimize the energy consumption in the base station of the secondary system energy consumption, is subject to different constraints of the main user system. We also evaluate the collaborative activity index of the sub-channel describing the activity of the primary users in the CCRN. The theoretical analyses and simulation results sufficiently demonstrate that the SE and EE relationship in the CCRN is not contrary and thus the achievement of optimal tradeoff between SE and EE. By making a rapprochement with a cognitive cellular network where SBSs adopts an equal power allocation strategy for sub-channels, the results of our proposed scheme indicate a significant improvement. Therefore, the model proposed in this paper offers a better tradeoff between SE and EE.
Immunomodulation as a Therapy for Aspergillus Infection: Current Status and Future Perspectives
Authors: Lauruschkat Chris D. , Einsele Hermann , Loeffler Juergen
Published: December 2018

Invasive aspergillosis (IA) is the most serious life-threatening infectious complication of intensive remission induction chemotherapy and allogeneic stem cell transplantation in patients with a variety of hematological malignancies. Aspergillus fumigatus is the most commonly isolated species from cases of IA. Despite the various improvements that have been made with preventative strategies and the development of antifungal drugs, there is an urgent need for new therapeutic approaches that focus on strategies to boost the host’s immune response, since immunological recovery is recognized as being the major determinant of the outcome of IA. Here, we aim to summarize current knowledge about a broad variety of immunotherapeutic approaches against IA, including therapies based on the transfer of distinct immune cell populations, and the administration of cytokines and antibodies.
DPYD, TYMS and MTHFR Genes Polymorphism Frequencies in a Series of Turkish Colorectal Cancer Patients
Authors: Amirfallah Arsalan , Calibasi Kocal Gizem , Unal Olcun Umit , Ellidokuz Hulya , Oztop Ilhan , Basbinar Yasemin
Published: December 2018

Fluoropyrimidine-based chemotherapy is extensively used for the treatment of solid cancers, including colorectal cancer. However, fluoropyrimidine-driven toxicities are a major problem in the management of the disease. The grade and type of the toxicities depend on demographic factors, but substantial inter-individual variation in fluoropyrimidine-related toxicity is partly explained by genetic factors. The aim of this study was to investigate the effect of dihydropyrimidine dehydrogenase (DPYD), thymidylate synthase (TYMS), and methylenetetrahydrofolate reductase (MTHFR) polymorphisms in colorectal cancer patients. Eighty-five patients who were administered fluoropyrimidine-based treatment were included in the study. The DPYD, TYMS and MTHFR polymorphisms were scanned by a next generation Sequenom MassARRAY. Fluoropyrimidine toxicities were observed in 92% of all patients. The following polymorphisms were detected: DPYD 85T>C (29.4% heterozygote mutants, 7.1% homozygote mutants), DPYD IVS 14+1G>A (1.2% heterozygote mutants), TYMS 1494del TTAAAG (38.4% heterozygote mutants, 24.7% homozygote mutants), MTHFR 677C>T (43.5% heterozygote mutants, 9.4% homozygote mutants) and MTHFR 1298A>C (8.2% heterozygote mutants, 2.4% homozygote mutants). A statistically significant association was demonstrated between MTHFR 677C>T and fluoropyrimidine-related toxicity (p value = 0.007). Furthermore, MTHFR 1298A>C was associated with hematopoietic toxicity (p value = 0.008). MTHFR polymorphisms may be considered as related factors of fluoropyrimidine toxicity and may be useful as predictive biomarkers for the determination of the colorectal cancer patients who can receive the greatest benefit from fluoropyrimidine-based treatments.
Analysis of Trauma Intensity during Surgical Bone Procedures Using NF-κB Expression Levels as a Stress Sensor: An Experimental Study in a Wistar Rat Model
Authors: Salles Marcos Barbosa , Allegrini Jr. Sergio , Yoshimoto Marcelo , Pérez-Díaz Leticia , Calvo-Guirado José Luis , Gehrke Sergio Alexandre
Published: December 2018

Aim and objectives: It is well known that the transcription factor NF-κB regulates multiple aspects of innate and adaptive immune functions and functions as a pivotal mediator of inflammatory responses. In the present study, we evaluated the trauma generated (inflammatory reaction) after osteotomy bone surgical procedures and placement of implants in the femoral cortical bone of Wistar rats. Surgical stress was evaluated measuring the release and activation of the NF-κB factor. Materials and Methods: Rats were divided into four groups (n = 10) and submitted to different surgical treatments: Control Group (G1 group), only bone perforation was performed without irrigation; Implant Group (G2 group), a titanium implant was inserted after bone perforation without irrigation; Irrigated Group (G3 group) perforations were performed with intense irrigation; and Vitaminic Compound Group (G4 group) surgical perforation was performed without irrigation and a vitaminic compound containing the principal ions present in the natural bone structure was used to fill the bone defect. All animals were euthanized six hours after the surgical procedure and NF-κB levels were determined through immunohistochemical stain followed by direct counting of labeled and unlabeled osteocytes. Results: Among different treated groups, the overall mean of the NF-κB positive cell count in all positions were higher for G1 group (33.4 ± 2.45 cells). NF-κB values were lower in the G2 group (28.9 ± 2.70 cells), whereas in the G3 group (24.3 ± 2.72 cells) as well as in G4 group still lesser NF-κB positive cells were counted (26.5 ± 2.60 cells). Conclusions: The results here presented suggest that maneuvers performed during osteotomy procedures can significantly affect inflammation levels. The NF-κB activation during the surgical procedures can be minimized and/or controlled thought the adequate irrigation or application of adequate substances.
Magneto-Dielectric Behaviour of M-Type Hexaferrite/Polymer Nanocomposites
Authors: Sanida Aikaterini , Stavropoulos Sotirios , Speliotis Thanassis , Psarras Georgios C.
Published: December 2018

In the present study two sets of nanocomposites consisting of an epoxy resin and BaFe12O19 or SrFe12O19 nanoparticles were successfully developed and characterized morphologically and structurally via scanning electron microscopy and X-ray diffraction spectra. The dielectric response of the nanocomposites was investigated by means of broadband dielectric spectroscopy and their magnetic properties were derived from magnetization tests. Experimental data imply that the incorporation of the ceramic nanoparticles enhances significantly the dielectric properties of the examined systems and their ability to store electrical energy. Dielectric spectra of all systems revealed the presence of three distinct relaxation mechanisms, which are attributed both to the polymer matrix and the nanoinclusions: Interfacial polarization, glass to rubber transition of the polymer matrix and the re-orientation of small polar side groups of the polymer chain. The magnetic measurements confirmed the ferromagnetic nature of the nanocomposites. The induced magnetic properties increase with the inclusion of hexaferrite nanoparticles. The nanocomposites with SrFe12O19 nanoparticles exhibit higher values of coercive field, magnetization, magnetic saturation and remanence magnetization. A magnetic transition was detected in the ZFC/FC curves in the case of the BaFe12O19/epoxy nanocomposites.
A Parameter-Based Ostrowski–Grüss Type Inequalities with Multiple Points for Derivatives Bounded by Functions on Time Scales
Authors: Kermausuor Seth , Nwaeze Eze R.
Published: December 2018

In this paper, we present some Ostrowski–Grüss-type inequalities on time scales for functions whose derivatives are bounded by functions for k points via a parameter. The 2D versions of these inequalities are also presented. Our results generalize some of the results in the literature. As a by-product, we apply our results to the continuous and discrete calculus to obtain some interesting inequalities in this direction.
CFD Investigation of the Effect of the Feed Spacer on Hydrodynamics in Spiral Wound Membrane Modules
Authors: Han Zhiming , Terashima Mitsuharu , Liu Bing , Yasui Hidenari
Published: December 2018

Spacers are designed to create a feed channel, but they are also obstacles to the flow in spiral wound membrane modules. The geometry of the feed spacer influences the flow pattern, which was investigated by using a three-dimensional Computational Fluid Dynamics (CFD) model. For the conventional feed spacer, unavoidable disadvantages were caused by its line contact with the membrane. The pillar-like feed spacer was designed to achieve area contact, which made it possible to enhance the porosity and minimize the adverse effects from the dead zone caused by the transverse filament. Through reductions in the connecting filament’s diameter, the channel porosity reached 0.979. Regarding the maximum porosity, the dimensionless power number was reduced by 47.31% at Reynolds number 150 in comparison with a previously studied commercial spacer. Furthermore, a modified friction factor, as a dimensionless parameter, was employed to investigate the shear stress at the membrane’s surface. At dimensionless power number 106, the enhancement of the modified friction factor increased by approximately 22.27% in comparison with the results of a previous study. Based on the numerical prediction, the homogenization of shear stress distribution, which changed the flow profile near the membrane, was featured through contour plots.
Antifouling Properties of PES Membranes by Blending with ZnO Nanoparticles and NMP–Acetone Mixture as Solvent
Authors: Ahmad Abdul Latif , Sugumaran Jayasree , Shoparwe Noor Fazliani
Published: December 2018

In this study, the antifouling properties of polyethersulfone (PES) membranes blended with different amounts of ZnO nanoparticles and a fixed ratio of N-methyl-2-pyrrolidone (NMP)-acetone mixture as a solvent were investigated. The properties and performance of the fabricated membranes were examined in terms of hydrophilicity, porosity, pore size, surface and cross-section image using scanning electron microscopy (SEM), surface roughness using atomic force microscopy (AFM), pure water flux, and humic acid filtration. Addition of ZnO as expected was found to improve the hydrophilicity as well as to encourage pore formation. However, the agglomeration of ZnO at a higher concentration cannot be avoided even when dissolved in a mixed solvent. The presence of highly volatile acetone contributed to the tight skin layer of the membrane which shows remarkable antifouling ability with the highest flux recovery ratio and negligible irreversible fouling. ZnO NPs in acetone/NMP mixed solvent shows an improvement in flux and rejection, but, the fouling resistance was moderate compared to the pristine membrane.
Diffusion Bonding of Ti2AlNb Alloy and High-Nb-Containing TiAl Alloy: Interfacial Microstructure and Mechanical Properties
Authors: Bian Hong , Lei Yuzhen , Fu Wei , Hu Shengpeng , Song Xiaoguo , Feng Jicai
Published: December 2018

In this study, reliable Ti2AlNb/high-Nb-containing TiAl alloy (TAN) joints were achieved by diffusion bonding. The effects of bonding temperature and holding time on the interfacial microstructure and mechanical properties were fully investigated. The interfacial structure of joints bonded at various temperatures and holding times was characterized by scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and X-ray diffraction (XRD). The results show that the typical microstructure of the Ti2AlNb substrate/O phase/Al(Nb,Ti)2 + Ti3Al/Ti3Al/TAN substrate was obtained at 970 °C for 60 min under a pressure of 5 MPa. The formation of the O phase was earlier than the Al(Nb,Ti)2 phase when bonding temperature was relatively low. When bonding temperature was high enough, the Al(Nb,Ti)2 phase appeared earlier than the O phase. With the increase of bonding temperature and holding time, the Al(Nb,Ti)2 phase decomposed gradually. As the same time, continuous O phase layers became discontinuous and the Ti3Al phase coarsened. The maximum bonding strength of 66.1 MPa was achieved at 970 °C for 120 min.
Mycoextraction: Rapid Cadmium Removal by Macrofungi-Based Technology from Alkaline Soil
Authors: Chen Miaomiao , Wang Likun , Hou Junliang , Yang Shushen , Zheng Xin , Chen Liang , Li Xiaofang
Published: December 2018

Fungi are promising materials for soil metal bioextraction and thus biomining. Here, a macrofungi-based system was designed for rapid cadmium (Cd) removal from alkaline soil. The system realized directed and rapid fruiting body development for subsequent biomass harvest. The Cd removal efficiency of the system was tested through a pot culture experiment. It was found that aging of the added Cd occurred rapidly in the alkaline soil upon application. During mushroom growth, the soil solution remained considerably alkaline, though a significant reduction in soil pH was observed in both Cd treatments. Cd and dissolved organic carbon (DOC) in soil solution generally increased over time and a significant correlation between them was detected in both Cd treatments, suggesting that the mushroom‒substratum system has an outstanding ability to mobilize Cd in an alkaline environment. Meanwhile, the growth of the mushrooms was not affected relative to the control. The estimated Cd removal efficiency of the system was up to 12.3% yearly thanks to the rapid growth of the mushroom and Cd enrichment in the removable substratum. Transcriptomic analysis showed that gene expression of the fruiting body presented considerable differences between the Cd treatments and control. Annotation of the differentially expressed genes (DEGs) indicated that cell wall sorption, intracellular binding, and vacuole storage may account for the cellular Cd accumulation. In conclusion, the macrofungi-based technology designed in this study has the potential to become a standalone biotechnology with practical value in soil heavy metal removal, and continuous optimization may make the system useful for biomining.
Fucoidan Exerts Anticancer Effects Against Head and Neck Squamous Cell Carcinoma In Vitro
Authors: Blaszczak Wiktoria , Lach Michal Stefan , Barczak Wojciech , Suchorska Wiktoria Maria
Published: December 2018

Fucoidans have been reported to exert anticancer effects with simultaneous low toxicity against healthy tissue. That correlation was observed in several cancer models, however, it has never been investigated in head and neck cancer before. To magnify the efficacy of conventional therapy, the administration of agents like fucoidan could be beneficial. The aim of this study was to evaluate the anticancer effect of Fucus vesiculosus (FV) extract alone and with co-administration of cisplatin in head and neck squamous cell carcinoma (HNSCC) in vitro. MTT assay results revealed an FV-induced inhibition of proliferation in all tested cell lines (H103, FaDu, KB). Flow cytometric cell cycle analysis showed an FV-induced, dose-dependent arrest in either S/G2 phase (H103, FaDu) or G1 arrest (KB). Furthermore, a dose-dependent gain in apoptotic fraction was observed. Western blot analysis confirmed the induction of apoptosis. A significant dose-dependent increase in reactive oxygen species (ROS) production was revealed in the H103 cell line, while FaDu cells remained unresponsive. On the contrary, an HPV-positive cell line, KB, demonstrated a dose-dependent decrease in ROS synthesis. Moreover, fucoidan enhanced the response to cisplatin (synergistic effect) in all cell lines with the HPV-positive one (KB) being the most sensitive. These results have been confirmed by flow-cytometric apoptosis analysis. In conclusion, we confirmed that fucoidan exhibits anticancer properties against HNSCC, which are manifested by the induction of apoptosis, regulation of ROS production, cell cycle arrest, and inhibition of proliferation.
Combined In Vitro Studies and in Silico Target Fishing for the Evaluation of the Biological Activities of Diphylleia cymosa and Podophyllum hexandrum
Authors: Rocha Marina Pereira , Campana Priscilla Rodrigues Valadares , Scoaris Denise de Oliveira , Almeida Vera Lucia de , Lopes Julio Cesar Dias , Shaw Julian Mark Hugh , Silva Claudia Gontijo
Published: December 2018

This paper reports the in silico prediction of biological activities of lignans from Diphylleia cymosa and Podophyllum hexandrum combined with an in vitro bioassays. The extracts from the leaves, roots and rhizomes of both species were evaluated for their antibacterial, anticholinesterasic, antioxidant and cytotoxic activities. A group of 27 lignans was selected for biological activities prediction using the Active-IT system with 1987 ligand-based bioactivity models. The in silico approach was properly validated and several ethnopharmacological uses and known biological activities were confirmed, whilst others should be investigated for new drugs with potential clinical use. The extracts from roots of D. cymosa and from rhizomes and roots of P. hexandrum were very effective against Bacillus cereus and Staphylococcus aureus, while podophyllotoxin inhibited the growth of Staphylococcus aureus and Escherichia coli. D. cymosa leaves and roots showed anticholinesterasic and antioxidant activities, respectively. The evaluated extracts showed to be moderately toxic to THP-1 cells. The chromatographic characterization indicated that podophyllotoxin was the major constituent of P. hexandrum extract while kaempferol and its hexoside were the main constituents of D. cymosa leaves and roots, respectively. These results suggest that the podophyllotoxin could be the major antibacterial lignan, while flavonoids could be responsible for the antioxidant activity.
Phyto-Mediated Photo Catalysed Green Synthesis of Silver Nanoparticles Using Durio Zibethinus Seed Extract: Antimicrobial and Cytotoxic Activity and Photocatalytic Applications
Authors: Sumitha Samuggam , Vasanthi Sethu , Shalini Sivadasan , Chinni Suresh V. , Gopinath Subash C.B. , Anbu Periasamy , Bahari Mohammed Baidi , Harish Rajak , Kathiresan Sathasivam , Ravichandran Veerasamy
Published: December 2018

In the present study, we have developed a green approach for the synthesis of silver nanoparticles (DSAgNPs) using aqueous extract of Durio zibethinus seed and determined its antibacterial, photocatalytic and cytotoxic effects. Surface plasmon resonance confirmed the formation of DSAgNPs with a maximum absorbance (λmax) of 420 nm. SEM and TEM images revealed DSAgNPs were spherical and rod shaped, with a size range of 20 nm and 75 nm. The zeta potential was found to be −15.41 mV. XRD and EDX analyses confirmed the nature and presence of Ag and AgCl. DSAgNPs showed considerable antibacterial activity, exhibited better cytotoxicity against brine shrimp, and shown better photocatalytic activity against methylene blue. Based on the present research work, it can be concluded that DSAgNPs could be used in the field of water treatment, pharmaceuticals, biomedicine, biosensor and nanotechnology in near future.
Analysis of Targeted Metabolites and Molecular Structure of Starch to Understand the Effect of Glutinous Rice Paste on Kimchi Fermentation
Authors: Jeong Duyun , Lee Jong-Hee , Chung Hyun-Jung
Published: December 2018

Bachu (Chinese cabbage) kimchi, a Korean traditional fermented dish, were prepared with or without the addition of glutinous (waxy) rice paste and their characteristics including pH, total bacteria count, total starch content, sugar metabolites, and molecular structure of starch were examined periodically for 20 days to investigate the effect of adding glutinous rice paste to kimchi during fermentation. The pH and total bacteria count showed that the fermentation of kimchi added with glutinous rice paste (GRP kimchi) progressed a little more quickly than that of control kimchi without glutinous rice paste. The GRP kimchi had higher glucose content but lower fructose content than control kimchi. Interestingly, maltose was only detected in GRP kimchi during fermentation. The GRP kimchi contained much greater amount of mannitol throughout fermentation than control kimchi. Total starch content in GRP kimchi gradually decreased during fermentation, which might have contributed to its greater glucose content and the larger amount of maltose production. In GRP kimchi, peak height and area for all degrees of polymerization (DP) of starch decreased during fermentation and its average chain length decreased while the proportion of short chains increased as fermentation processed, indicating degradation of starch chains by enzymes presented in the kimchi.
Fabrication of Si/graphene/Si Double Heterostructures by Semiconductor Wafer Bonding towards Future Applications in Optoelectronics
Authors: Naito Takenori , Tanabe Katsuaki
Published: December 2018

A Si/graphene/Si planar double heterostructure has been fabricated by means of semiconductor wafer bonding. The interfacial mechanical stability and interlayer electrical connection have been verified for the structure. To the best of our knowledge, this is the first realization of a monolayer-cored double heterostructure. In addition, a double heterostructure with bilayer graphene has been prepared for bandgap generation and tuning by application of a bias voltage. These structures move towards the realization of versatile graphene optoelectronics, such as an electrically pumped graphene laser. Our Si/graphene/Si double heterostructure is positioned to form a new basis for next-generation nanophotonic devices with high photon and carrier confinements, earth abundance (C, Si), environmental safety (C, Si), and excellent optical and electrical controllability by silicon clads.
Electrochemical Enantiomer Recognition Based on sp3-to-sp2 Converted Regenerative Graphene/Diamond Electrode
Authors: Gao Jingyao , Zhang Haoyang , Ye Chen , Yuan Qilong , Chee Kuan W. A. , Su Weitao , Yu Aimin , Yu Jinhong , Lin Cheng-Te , Dai Dan , Fu Li
Published: December 2018

It is of great significance to distinguish enantiomers due to their different, even completely opposite biological, physiological and pharmacological activities compared to those with different stereochemistry. A sp3-to-sp2 converted highly stable and regenerative graphene/diamond electrode (G/D) was proposed as an enantiomer recognition platform after a simple β-cyclodextrin (β-CD) drop casting process. The proposed enantiomer recognition sensor has been successfully used for d and l-phenylalanine recognition. In addition, the G/D electrode can be simply regenerated by half-minute sonication due to the strong interfacial bonding between graphene and diamond. Therefore, the proposed G/D electrode showed significant potential as a reusable sensing platform for enantiomer recognition.
A Region Merging Segmentation with Local Scale Parameters: Applications to Spectral and Elevation Data
Authors: Dekavalla Maria , Argialas Demetre
Published: December 2018

Region merging is the most effective method for the segmentation of remote sensing data. The quality and the size of the resulted image objects is controlled by a global heterogeneity threshold, termed as the scale parameter. However, the multidimensional nature of the visible features in a scene defies the use of an even optimum single global scale parameter. In this study, a novel region merging segmentation method is proposed, where a local scale parameter is defined for each image object by its internal and external heterogeneity measures (i.e., local variance and Moran’s I). This method allows image objects with low internal and external heterogeneity to be further merged with higher scale parameter values, since they are more likely to be a part of an adjacent object, than objects with high internal and external heterogeneity. The proposed method was applied in spectral and elevation data and its results were evaluated visually and with supervised and unsupervised evaluation methods. The comparison with multi-resolution segmentation (MRS) showed that the proposed region merging method can produce improved segmentation results in terms of maximizing intra-object homogeneity and inter-object heterogeneity as well as in the delimitation of specific target objects, present in spectral and elevation data. The unsupervised evaluation results of the (1) Côte d’Azur, (2) Manchester, and (3) Szada images from the SZTAKI-INRIA building detection dataset showed that the proposed method (overall goodness, OGf (1): 0.7375, (2): 0.7923, (3): 0.7967) performs better than MRS (OGf (1): 0.7224, (2): 0.7648, (3): 0.7823). The higher values of OGf indicate their ability to produce segmentation results with reduced over-segmentation effects and without the need of presegmented input data, in contrast to the objective heterogeneity and relative homogeneity (OHRH) hybrid segmentation method (OGf (1): 0.5864, (2): 0.5151, (3): 0.6983).
A Comparative Assessment of Different Modeling Algorithms for Estimating Leaf Nitrogen Content in Winter Wheat Using Multispectral Images from an Unmanned Aerial Vehicle
Authors: Zheng Hengbiao , Li Wei , Jiang Jiale , Liu Yong , Cheng Tao , Tian Yongchao , Zhu Yan , Cao Weixing , Zhang Yu , Yao Xia
Published: December 2018

Unmanned aerial vehicle (UAV)-based remote sensing (RS) possesses the significant advantage of being able to efficiently collect images for precision agricultural applications. Although numerous methods have been proposed to monitor crop nitrogen (N) status in recent decades, just how to utilize an appropriate modeling algorithm to estimate crop leaf N content (LNC) remains poorly understood, especially based on UAV multispectral imagery. A comparative assessment of different modeling algorithms (i.e., simple and non-parametric modeling algorithms alongside the physical model retrieval method) for winter wheat LNC estimation is presented in this study. Experiments were conducted over two consecutive years and involved different winter wheat varieties, N rates, and planting densities. A five-band multispectral camera (i.e., 490 nm, 550 nm, 671 nm, 700 nm, and 800 nm) was mounted on a UAV to acquire canopy images across five critical growth stages. The results of this study showed that the best-performing vegetation index (VI) was the modified renormalized difference VI (RDVI), which had a determination coefficient (R2) of 0.73 and a root mean square error (RMSE) of 0.38. This method was also characterized by a high processing speed (0.03 s) for model calibration and validation. Among the 13 non-parametric modeling algorithms evaluated here, the random forest (RF) approach performed best, characterized by R2 and RMSE values of 0.79 and 0.33, respectively. This method also had the advantage of full optical spectrum utilization and enabled flexible, non-linear fitting with a fast processing speed (2.3 s). Compared to the other two methods assessed here, the use of a look up table (LUT)-based radiative transfer model (RTM) remained challenging with regard to LNC estimation because of low prediction accuracy (i.e., an R2 value of 0.62 and an RMSE value of 0.46) and slow processing speed. The RF approach is a fast and accurate technique for N estimation based on UAV multispectral imagery.
Accuracies Achieved in Classifying Five Leading World Crop Types and their Growth Stages Using Optimal Earth Observing-1 Hyperion Hyperspectral Narrowbands on Google Earth Engine
Authors: Aneece Itiya , Thenkabail Prasad
Published: December 2018

As the global population increases, we face increasing demand for food and nutrition. Remote sensing can help monitor food availability to assess global food security rapidly and accurately enough to inform decision-making. However, advances in remote sensing technology are still often limited to multispectral broadband sensors. Although these sensors have many applications, they can be limited in studying agricultural crop characteristics such as differentiating crop types and their growth stages with a high degree of accuracy and detail. In contrast, hyperspectral data contain continuous narrowbands that provide data in terms of spectral signatures rather than a few data points along the spectrum, and hence can help advance the study of crop characteristics. To better understand and advance this idea, we conducted a detailed study of five leading world crops (corn, soybean, winter wheat, rice, and cotton) that occupy 75% and 54% of principal crop areas in the United States and the world respectively. The study was conducted in seven agroecological zones of the United States using 99 Earth Observing-1 (EO-1) Hyperion hyperspectral images from 2008–2015 at 30 m resolution. The authors first developed a first-of-its-kind comprehensive Hyperion-derived Hyperspectral Imaging Spectral Library of Agricultural crops (HISA) of these crops in the US based on USDA Cropland Data Layer (CDL) reference data. Principal Component Analysis was used to eliminate redundant bands by using factor loadings to determine which bands most influenced the first few principal components. This resulted in the establishment of 30 optimal hyperspectral narrowbands (OHNBs) for the study of agricultural crops. The rest of the 242 Hyperion HNBs were redundant, uncalibrated, or noisy. Crop types and crop growth stages were classified using linear discriminant analysis (LDA) and support vector machines (SVM) in the Google Earth Engine cloud computing platform using the 30 optimal HNBs (OHNBs). The best overall accuracies were between 75% to 95% in classifying crop types and their growth stages, which were achieved using 15–20 HNBs in the majority of cases. However, in complex cases (e.g., 4 or more crops in a Hyperion image) 25–30 HNBs were required to achieve optimal accuracies. Beyond 25–30 bands, accuracies asymptote. This research makes a significant contribution towards understanding modeling, mapping, and monitoring agricultural crops using data from upcoming hyperspectral satellites, such as NASA’s Surface Biology and Geology mission (formerly HyspIRI mission) and the recently launched HysIS (Indian Hyperspectral Imaging Satellite, 55 bands over 400–950 nm in VNIR and 165 bands over 900–2500 nm in SWIR), and contributions in advancing the building of a novel, first-of-its-kind global hyperspectral imaging spectral-library of agricultural crops (GHISA: www.usgs.gov/WGSC/GHISA).
Global Land Surface Temperature Influenced by Vegetation Cover and PM2.5 from 2001 to 2016
Authors: Song Zengjing , Li Ruihai , Qiu Ruiyang , Liu Siyao , Tan Chao , Li Qiuping , Ge Wei , Han Xujun , Tang Xuguang , Shi Weiyu , Song Lisheng , Yu Wenping , Yang Hong , Ma Mingguo
Published: December 2018

Land surface temperature (LST) is an important parameter to evaluate environmental changes. In this paper, time series analysis was conducted to estimate the interannual variations in global LST from 2001 to 2016 based on moderate resolution imaging spectroradiometer (MODIS) LST, and normalized difference vegetation index (NDVI) products and fine particulate matter (PM2.5) data from the Atmospheric Composition Analysis Group. The results showed that LST, seasonally integrated normalized difference vegetation index (SINDVI), and PM2.5 increased by 0.17 K, 0.04, and 1.02 μg/m3 in the period of 2001–2016, respectively. During the past 16 years, LST showed an increasing trend in most areas, with two peaks of 1.58 K and 1.85 K at 72°N and 48°S, respectively. Marked warming also appeared in the Arctic. On the contrary, remarkable decrease in LST occurred in Antarctic. In most parts of the world, LST was affected by the variation in vegetation cover and air pollutant, which can be detected by the satellite. In the Northern Hemisphere, positive relations between SINDVI and LST were found; however, in the Southern Hemisphere, negative correlations were detected. The impact of PM2.5 on LST was more complex. On the whole, LST increased with a small increase in PM2.5 concentrations but decreased with a marked increase in PM2.5. The study provides insights on the complex relationship between vegetation cover, air pollution, and land surface temperature.