bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of the Market Position of Natural Gas in China Based on Energy Production and Consumption Elasticity
Authors: Zhang Ce Ming , Peng Shi Ni
Published: September 2011

In this paper, five different energy of cross price elasticity were calculated, the substitutional relation between different energy sources in China were discussed. The status of natural gas in energy market was analysed. The results show that only LNG take a role of complements to natural gas, while electricity, gasoline, diesel oil and coal have alternative relations with natural gas. Besides,electricity has the strongest ability to substitute natural gas. Conversely, if it is possible for natural gas to replace other energy sources , LNG will be the best substitute and be followed by gasoline,diesel oil,and power. And the natural gas has the weakest ability to substitute coal in China.
Photosensitization of Nitrogen Doped TiO2 Nanoparticles with Na-Chlorophyllin Copper for Degradation of Methyl Orange
Authors: Gong Jian Yu , Yang Chang Zhu , Pu Wen Hong , Zhang Jing Dong
Published: September 2011

Nitrogen doped titanium dioxide nanoparticals (N-TiO2) were prepared by the sol-hydrothermal method using urea as N sources. SEM showed the sphericity of as-prepared nanoparticals. XRD indicated that N-TiO2 was anatase crystal after thermal treatment. While Na-chlorophyllin copper (Na-chl-Cu) was used as to sensitize the N-TiO2, the photocurrent of Na-chl-Cu/N-TiO2 was 50 ìA double than that of N-TiO2 under visible light illumination. Thus, the visible light photoelectrocatalytic degradation properties of Na-chl-Cu/N-TiO2 were investigated using methyl orange (MO) as the objective pollution. When 1.8 V anodic bias potential and visible light were simultaneously applied, the highest degradation efficiency of MO over the Na-chl-Cu/N-TiO2 was obtained.
Comparative Evaluation of the Chemical Composition of Essential Oil from Twig, Leaf and Root of Clerodendrum inerme (L.) Gaertn
Authors: Li Dong Hai , Wang Zhao , Liang Zhen Yi , Yang Xiao Bo , Xu Jing
Published: September 2011

This study was designed to comparative evaluate the chemical composition of the essential oil from twig, leaf and root parts ofClerodendrum inerme(L.)Gaertn. GC-MS analyses of the oils revealed the presence of 8, 8 and 6 compounds in the essential oils obtained by hydrodistillation from different parts, respectively. Higher amounts of esters were found to compose a major chemotype in diverse parts of the plant. Dibutyl phthalate was the main component of all the essential oils attaining in average 34.22%, 59.28% and 44.27% and the other kind of esters accounted for 38.30%, 17.28% and 10.89% in the twig, leaf and root parts, respectively. In addition, some pharmaceutical components such as stigmasterol, linoleic acid and ferruginol were discovered. The study offers theoretic basis for utilization of the traditional folk herbC. inerme.
Study on Mercury Adsorption Performance of Modified Fly Ash
Authors: Zhu Ting Yu , Kuang Jun Yan , Xu Wen Qing , Ye Meng , Guo Yang Yang , Liu Wen
Published: September 2011

The fly ash was modified by Cl, Fe, Cu salts to investigate the mercury adsorption performance and analyze the impacts on it. Experiment results indicate that significant improvement of removal efficiencies with FeCl3、CuCl2 and CuBr2 impregnation onto fly ash. The modification adjusted the pore structure of fly ash and formed more pores to absorb Hg, also it was found that new active sites were generated after the treatment, which can oxidize Hg0 and improve chemical adsorption.
COMSOL Simulations for Flow Channels of Low-Temperature Fuel Cell
Authors: Chung Chin Ming , Lin Ching Huei , Lin Che Wei
Published: September 2011

A multiple physical finite elements to analyze software (COMSOL Multiphysics) is employed to investigate serpentine flow channels of a small proton exchange membrane fuel cell with power 0.5W. Distributions of the oxygen flow, the liquid water, and the electric current density in three different designed flow channels are simulated and compared. Results show that increasing the channel width is conducive to increase the oxygen flow and reduce the production of liquid water. It thus enhances the electric current density. However, it will reduce the oxygen flow, produce more liquid water, and depress the current density if the channel width is decreased.
Using Biological Process to Destroy Lignocellulosic Structure for Energy Conversion
Authors: Li Po Min , Lee Ching Wei , Lin Jhan Shan
Published: September 2011

Lignocellulose is one of the richest biological resources on earth. Yet, due to the robust structure, lignocelluloses are still not effectively utilized. This research adopted the fungi from the nature—Corilus versicolor—to culture and destroy the lignocellusic structure. The object of the research was banana stalks, a common Taiwan agricultural waste. The banana stalks were physically pretreated to banana wood flour before use. Through changes of the grains’ sizes of the banana wood flour, and proportions of the compounded carbons of the banana wood flour in the media, the optimal degradation activity was determined. The result showed that on the 12th day the 5mm grains used to replace the compounded carbons reached the maximal microbial activity, 70 times of a general medium. On the 14th day near 18% of celluloses were consumed by white-rot fungi. The result has contribution to lignocellulosic structure breakdown and a great step for energy conversion in subsequent stage.
Electron Microscopic Studies on Phosphate Binding Processes in the Presence of Iron
Authors: Subasinghe Nalaka Deepal
Published: September 2011

Phosphates are widely used as a substitute material for bones and teeth in medical sciences. It is known that phosphate and iron have a strong affinity for each other. In this study, process of formation of iron phosphate was closely monitored using scanning electron microscope equipped with backscatter electron image and energy dispersive X-ray imaging facilities. Different stages of formation of the iron-phosphate material in an environment rich in phosphate and iron were observed. Initial stage of absorbing iron on phosphate-rich substrate is the most important stage of the entire process. X-ray mapping provides strong visual evidence to track down the dispersion of major elements during this process.
Compressive Strength of Binary and Ternary Blended Cement Mortars Containing Fly Ash and Silica Fume under Autoclaved Curing
Authors: Wongkeo Watcharapong , Thongsanitgarn Pailyn , Chaipanich Arnon
Published: September 2011

Cement industry is a one of the major sources of environmental pollution therefore the reduction of cement demand should be improved. Fly ash and silica fume is a by-product of industries and it should be reused to reduce the waste pollution. Thus, this study investigated the use of fly ash and silica fume as a cement replacement in binary and ternary blended cements on compressive strength and physical properties of mortar. Autoclaved curing at 130 °C and 20 psi of pressure for 9 h was used in this study. The results show that the compressive strength of binary blended cement mortar with FA tends to decrease with increased FA replacement and shows compressive strength lower than PC control. However, compressive strength of binary blended cement mortar with SF was improved and shows compressive strength higher than that of PC control. The compressive strength of ternary blended cement mortar was higher than binary blended cement at the same level replacement and it increases with increased SF replacement. Moreover, ternary blended cement mortar containing 10%SF by weight contribute in giving compressive strength higher than PC control. The incorporation of FA with SF can enhance workability of blended cement mortar containing only SF replacement.
Power Control Based on Pub/Sub for DFIG Wind Farm
Authors: Qiao Ling Ling , Tian Zhi Gang
Published: September 2011

Power control in large wind farm has a big impact on the Grid, especially in low connectivity ratio. Publish subscribe technology is asynchronous loosely coupled, and multi-to-multipoint communication as adapted for large distributed real-time control of network communications needs in the wind farm. This paper presents publish subscribe-based real-time power command-delivery method. It is well used to deliver the control of wind farm, reducing the time-delay in control loops. Simulation results show that the method further improves the quality of the power control of common coupling in large wind farms.
Migration Patterns of Heavy Metals in Soil in Wastewater Irrigation Area in the Suburb of Eastern Inner Mongolia
Authors: Hu Ri Cha , Sun Li Bo
Published: September 2011

Based on the soil test of heavy metals in wastewater irrigation area in eastern Inner Mongolia, this paper analyses the content of heavy metals Cr, Cu, Cd, Pb in different layers of soil samples and reveals the internal relations between heavy metal concentration gradient in the vertical direction and soil properties, thus providing a scientific basis to guide sewage irrigation, solve the problem of water shortage in wastewater irrigation area and carry out environmental impact assessment. Soil is an important subsystem in natural environmental systems, through which many pollutants, especially heavy metals, produced by human activities, enter the food chain and ultimately do harm to human health. Currently, heavy metal ion contamination to the soil caused by wastewater irrigation has become one of the focuses in the study of soil chemistry and agricultural environment pollution. For this reason, it is particularly important to study the migration mechanism of heavy metals in soil and to provide a reliable theoretical basis for the prevention and management of soil contaminants.
The Involvement of Nitric Oxide and Hydrogen Peroxide in eATP-Inhibited Pollen Germination and Tube Growth of Paulownia Tomentosa In Vitro
Authors: Zhang Yuan Hua , She Xiao Ping
Published: September 2011

Role of nitric oxide(NO) and hydrogen peroxide(H2O2) in eATP-inhibited reduction of in vitro pollen germination and tube growth of Paulownia tomentosa Steud. were studied. Results showed that exposure of the pollen to a series of eATP (0.2, 0.4, 0.6, 0.8, 1.0 mM) concentration for 3h resulted in not only the reduction of pollen germination but also the reduction of tube growth. Also, NOS inhibitor NG-nitro-L-Arg-methyl eater (L-NAME ), NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3- oxide(c-PTIO) and two scavengers of H2O2, ascorbic acid (ASC) and catalase (CAT) not only largely prevented eATP-inhibited pollen germination but also tube growth. These results indicated that NO and H2O2are involved in eATP inhibited pollen germination and tube growth.
Developmental Changes of Growth Hormone Mrna Abundance in Pituitary Tissue of Jinhua and Landrace Gilts
Authors: Miao Zhi Guo , Li Guo Wang , Wang Shi Zhu , Chang Xin Yao , Xie Hong Bing , Liu Chang Zhong
Published: September 2011

In this pepar we investigated the developmental patterns of expression of growth hormone (GH) gene in pituitary tissue in pigs of different breeds and their effects on the carcass fat contents. 3 Jinhua gilts and 3 Landrace gilts were sampled at 35, 80 and 125 days of age, respectively. Carcass fat contents were determined. Pituitary tissue was sampled and total RAN was extracted to determine GH mRNA expression levels by semi-quantitative RT-PCR. The results showed that the contents of carcass fat increased with growth and showed significant differences (P﹤0.05) between different age groups in the two breeds. Furthermore, carcass fat contents in Jinhua gilts were higher than that in Landrace gilts during growth (P﹤0.05). GH mRNA expression levels decreased with age and displayed breed differences. Jinhua gilts showed lower abundance of GH mRNA compared with Landrace gilts at 35, 80 and 125 days of age (P﹤0.05). In addition, GH mRNA expression level was negatively related to carcass fat content in Jinhua and Landrace gilts (r = -0.790 (P = 0.01), r = -0.755 (P = 0.02), respectively).
SPSS for Water Pollution Contribution of Tonghui River in Beijing Based on Principal Component Analysis
Authors: Zhang Li Ping , Pang Jie , Ke Lei , Shi Yuan Yuan , Yang Mu Qi , Hu Qian Yu , Guo Yuan Jie
Published: September 2011

This paper researches on sewage discharge condition along Tonghui River in Beijing. According to stationing principle and field investigation, there are 18 sewage outlets along Tonghui River and select 7 main water quality pollution indicators and sewage flow to assess the sewage outlets’ pollution distribution. By means of SPSS soft applied in principal component analysis method, paper analysis on the main pollution indicators and the main pollution contribution sewage outlets. The result shows 3 extracted principal components reflect 88.92% information of primitive variables. Then solve the formula of the 3 extracted principal components as F1, F2, F3. According to the contribution percentage of variance in table Ⅲ, the function of comprehensive evaluation expression can be deduced as F= 0.727F1+0.187F2+0.086F3. The results show that among the 18 sewage outlets, the worst pollution contribution is No.16 outlet, the 18 sewage outlets’ pollution contribution to Tonghui River is in the order of NO.16> NO.11> NO.12> NO.3> NO.8> NO.9> NO.13> NO.14> NO.17> NO.10> NO.15> NO.6> NO.18> NO.5> NO.1> NO.7> NO.4> NO.2. The assessment result is tally with the actual situation. Administrative means, technical means and management means are the effective measures to improve water quality of Tonghui River.
Research on the Characteristics and Culture Conditions of Saccharomyces boulardii
Authors: Du Li Ping , Hao Rui Xue , Xiao Dong Guang , Guo Li Li , Gai Wei Dong
Published: September 2011

The morphological, physiological and biochemical characteristics of Saccharomyces boulardii were studied for the preparation of highly active yeast. The optimal condition of the flask culture including inoculum size, culture temperature, pH value, were investigated separately. The results showed that S.boulardii was asporogenous and had better heat tolerance and acid tolerance growing well at 37°C and pH 2.0. We also found that thermal death temperature was 55~56°C and S.boulardii is well ethanol-tolerant, for the highest concentration of ethanol that S.boulardii can tolerate was 20%. The optimal culture conditions in a flask culture was YEPD medium with 50ml/250ml medium volume, natural pH, 5% inoculum size, 32°C culture temperature, 160 r/min shaker speed, and 24 hours cultivation period. Under these conditions, suspension was diluted 10 times and the optical density at 600 nm was up to 0.85.
Effect of Pyruvate and Lactic Acid on the Development of Porcine Embryos
Authors: Fu Bo , Liu Di , Ma Hong , Li Zhong Qiu , Wang Liang , Bai Jing
Published: September 2011

This study aimed to investigate the effects of pyruvate and lactic acid on the earlier development of porcine embryos. 5.56 mmol/L glucose in culture medium (NCSU-23) was replaced with 0.2 mmol/L pyruvate and 5.7 mmol/L lactic acid, namely mNCSU-23. Parthenogenetic embryos and nuclear transferred embryos were transferred into NCSU-23 or mNCSU-23 medium according to the experimental design. Parthenogenetic embryos and nuclear transferred embryos were evaluated for the numbers of 5-8 cells stage on Day 2. Blastocyst rates and the numbers of nuclei in the blastocyst were determined on Day 6. From the results, we observed a higher proportion of the embryos reaching 5-8 cells stage at 48h post-activation in mNCSU/NCSU treatment than control during the first part of in vitro culture(IVC) (P < 0.05). In addition, we also observed that the rates of blastocysts formation in mNCSU/NCSU treatments on Day 6 were significantly higher than other treatments, with mNCSU-23/mNCSU-23 treatment having the lowest rates of blastocysts formation on Day 6 (P < 0.05). Our results have demonstrated that replacing glucose with pyruvate and lactic acid during the first part of IVC may be beneficial to the development of the porcine embryos and stepwise culture system(mNCSU-23/NCSU-23) was optimal.
Romoval of Zinc Ion from Aqueous Solution by Adsorption onto Solidifying Landfilled Sludge
Authors: Zhu Wei Qin , Zhang Zhi , Shan Jian Li
Published: September 2011

In this paper, the removal behaviour of Zn2+from aqueous solution by adsorption onto solidifying landfilled sludge and its mechanism were studied. The results showed that Zn2+adsorption capacity reached optimum when initial pH was 3.0. When initial Zn2+concentration was150mg/L, the removal efficiency could reach more than 90% with dosage of solidifying landfilled sludge being 6g/L. Moreover, the adsorption isotherm could conform to Freundlich equations best. The desorption ratio for adsorbed Zn2+reached above 90% by HCl solution with pH value being 1.0, however, it was only about 10% despite of EDTA concentration. FTIR analysis showed that main site for Zn2+adsorption onto solidifying landfilled sludge was Me-O group from metal oxide and hydroxyl group from fatty alcohol.
Tellurium Extraction from the Unique Independent Tellurium Ores in China by Bioleaching
Authors: Guo Ya Fei , Deng Tian Long , Zhang Nan , Liao Meng Xia
Published: September 2011

This study was designed to investigate tellurium bioleaching from the unique independent low-grade tellurium ores containing tetradymite, joseite, pyrite, chalcopyrite, silica, and a minor amount of dolomite, which is the first discovered scattered element deposit in the world. For the bioleaching, the tellurium extractions from the low-grade ores by with and without the adapted bacteria of acidophilic Thiobacillus ferrooxidans were examined. The effects of pH, pulp density, and temperature for bioleaching were studied systematically. After 20 days of bioleaching at 30°C, an 80 % of the tellurium was extracted in laboratory study. Leaching kinetics indicated that diffusion through the product layer was the rate controlling process during tellurium bioleaching, and the relative activation energies during tellurium bioleaching stage was calculated to be 34.75 kJ/mol.
Association of Prkag3 Gene Polymorphisms with Meat Quality Traits in Chinese Meat-Type Quality Chicken Populations
Authors: Yin Hua Dong , Zhao Xiao Ling , Zhang Zhi Chao , Zhou Na Na , Zhu Qing
Published: September 2011

In this study, we aimed to detect the single nucleotide polymorphism (SNPs) of the chicken PRKAG3 gene and discern the potential association with meat quality traits. A total of 240 meat-type quality chickens were screened by using single-strand conformational polymorphism analysis (SSCP) and DNA sequencing. Two SNPs (g.1744G>C and g.3207A>G) were identified in chicken PRKAG3 with medium polymorphism (0.25C were significantly associated with water content, crude protein, and crude ash (P<0.05/0.01). The SNP genotypes g.3207A>G were significantly associated with water content, and crude protein (P<0.01). The results suggested that PRKAG3 gene is potential major genes or is in close linkage disequilibrium with the QTL affecting meat quality traits in this population of chickens.
Visual Analysis on the Research of Oil Crop
Authors: Zhao Rong Ying , Chen Rui , Dang Zhan Hai , Dang Zhao , Zhang Gui
Published: September 2011

Oil crops occupy an important position in the world economy in the past twenty-five years. The production of oil crops has increased twice and oil products are mainly used for food, fodder, mechanical, smelt, soap, paint, rubber, plastic, medical and other areas. Information visualization provides new ways for scientometrics and knowmetrics in recent years. This paper does the visual analysis of international oil crop. Based on analytic techniques such as co-citation analysis, co-appearance analysis and information visualization, this paper maps the co-citation network, hot words of most cited papers and front words of oil crop using the CiteSpace software. It identifies the content of high cited documents, analyzes the research focus of international oil crop.
Genetic Differentiation and Systematic Evolution of Sichuan Rhesus Macaques
Authors: Li Di Yan , Yao Yong Fang , Cheng An Chun , Xu Huai Liang , Ni Qing Yong , Zeng Wen , Bi Feng Jun , Yang Ze Xia , Chen Xiao Yue
Published: September 2011

The rhesus macaque (Macaca mulatta) is perhaps the most widely distributed nonhuman primate, is used more extensively than any other primate species as animal models for the study of biomedicine. In this study, we sequenced and analysed a fragment of 491 bp of mitochondrial DNA (mtDNA) partial control region in 230 individuals belonging to 8 populations of Sichuan province of China. Among the 230 individuals, we observed 56 different haplotypes defined by 110 polymorphic sites. The average number of nucleotide differences (k) was 18.844, indicating that very high genetic differentiation and the extant Sichuan wild rhesus macaques population consists of diverse genetic groups in their maternal lineage. The mtDNA haplotypes of these Sichuan wild rhesus macaques clustered into two main clades (CladeⅠand Clade Ⅱ), may correspond toM. m. vestitusandM.m.lasiotis.
Optimization of the Sporogenous Medium of Irpex Lacteus Using Response Surface Methodology
Authors: Zhang Na , Chen Qing Yan , Pan Ruo Chuan , Yao Xue Feng , Wang Yan Feng , Lu Jia Hui , Teng Li Rong
Published: September 2011

Response surface methodology (RSM) was used to optimize sporulation medium components for improving spores concentration in I. lacteus fermentation broth. Five factors of sporulation medium components, glucose (X1), peptone (X2), yeast extract (X3) MgSO4·7H2O (X4) and VB1 (X5), were chosen to optimize through a five-level, five-factor central composite design (CCD) base on single factor experiments. The concentration of medium components which influenced the sporulation of I. lacteus was further optimized using RSM. The results show that The medium that consists of 4.15 g/L peptone, 2.04 g/L yeast extract, 5.10 g/L glucose, 0.17 g/L MgSO4·7H2O and 0.03 g/L VB1 enhance spores concentration significantly . The final spores concentration (2.19×105 /mL) by using the optimized medium was about five times higher than normal medium.
Lab Studies of MEOR Strains Optimization for High Salinity Reservoirs
Authors: Wu Jing Chun , Lv Xiao Ming , Ou Zhi Jie , Yin Dai Yin
Published: September 2011

.Aiming at the high salinity at low-permeability reservoirs, a strain named S-4 that can yield a kind of bio-surfactants was screened from the produced water of oil wells in use of the Hemolysis of the bio-surfactants and its characteristics of making the color of blue gel plate change in its formation process. The laboratory results show that the surface tension of the strains fermentation liquor becomes less than 30mN/m after cultured by crude oil as the carbon source, and it would remain at the value of 31mN/m even if the concentration becomes a half; this bio-surfactant keeps stable when the salinity is in the range of 4000mg/L～50000mg/L; In physical experiments the strains can improve crude oil recovery ratio by 4% which shows a good oil displacement effect.
Application of QuEchERS Method in Determining Imidaclothiz Residue in Soil and Citrus by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry
Authors: Qin Li , Liu Fu Guang , Wu Hui Ming , Zhu Guo Nian
Published: September 2011

A simple method for the determination of imidaclothiz residue in soil and citrus samples was established by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring (MRM) mode. Imidaclothiz was extracted from soil and citrus samples using a procedure known as the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. Experiments on 3 fortification concentrations were carried out, and the limit of determination is 1.0×10-10g. The recoveries ranging from 80.7% to 118.9% with high correlation coefficient (r>0.99) were obtained within the linear range (0.01-1.0mg/kg). Based on the above method, we also carried out field trials. Results showed that the degradation of imidaclothiz coincided with the fitted equations Ct=0.1028e-0.0266t, Ct=0.1778e-0.0311tin soil and Ct=1.3612e-0.0632t, Ct=0.7162e-0.0968tin citrus, respectively, with the average half-lives of 24.2 d in soil and 9.1 d in citrus, respectively. The results indicate that the developed method is fast, accurate, and easy to operate. It also demonstrates that the method can meet the requirements of determination of imidaclothiz in soil and citrus.
Toxicity of Fe0 Nanoparticles on the Denitrifying Bacteria-Alcaligenes Eutrophus
Authors: Dong Mei Ying , Wang Xue , Huang Fang , Jin Zhao Hui , Li Tie Long
Published: September 2011

The bio-toxicity of Fe0 nanoparticles on the denitrifying bacterial- Alcaligenes eutrophus was detected by the two methods of detecting inhibition of the growth of microorganisms and nitrification inhibition rate. The results showed that Fe0 nanoparticles had obvious toxicity on the growth and nitrification inhibition rate. Besides, there had distinct relationship between dose and toxicity of Fe0 nanoparticles. Meanwhile, we also studied effects of different kinds of Fe0 nanoparticles on microorganism, including bimetallic nanoparticles(nano Fe–Ni) and coated iron nanoparticles (sodium oleate–Fe0nanoparticles). We detected that the bio-toxicity of these three Fe0 nanoparticles followed the sequence of Ni-containing nanoparticles>unmodified NZVI>the sodium oleate–Fe0 nanoparticles. In conclusion, Fe0 nanoparticles had visible toxicity on the denitrifying bacterial, but we can decrease the toxicity of Fe nanoparticles by surface coating and decoration of nano-metal materials.
Extraction of Pectin from Pomelo Peel
Authors: Ma Yu Xiang , Liu Ben Guo , Hu Liang Bin , Wang Miao Yan
Published: September 2011

The extraction of pectin from pomelo peel was optimized to maximize pectin yield Y in this study. A central composite design of response surface methodology involving extracting time, extracting temperature, liquid-solid ratio and pH was used, and second-order model for Y was employed to generate the response surfaces. The optimum condition for Y was determined as follows: extracting time 1h, extracting temperature 95 °C, liquid-solid ratio 25, pH 1. Under the optimum condition, the pectin yield was 20.98%.
Probiotic Characteristics of Conjugated Linoleic Acid Producing Bacteria
Authors: Li Jing Yan , Zhang Lan Wei , Guo Chun Feng , Yi Hua Xi , Zhang Ying Chun , Li Qi
Published: September 2011

The aim of this study was to select CLA producing bacteria from fermented diary products and human faeces, then evaluate their resistance to simulated gastrointestinal stress and adhesiveness to human intestinal epithelial cells according typically assess methods. Additionally, hydrophobicity, resistance to opportunistic pathogens and phenol ability were analysed. Results showed that three L. paracasei subp. Paracasei (F0721, IN 5.22 and Q9), two Lactobacillus acidophilus (F0221 and M6) and one Lactobacillus plantarum (G14) exert the ability to product conjugated linoleic aicd ranging from 77.21 to 111.59 μg/ml in the presence of 0.5 mg/ml linoleic acid (LA) in MRSc broth under anaerobic conditions at 37°C for 40 h. Strain F0721, IN5.22 and G14 showed higher resistance to gastric acid than other strains at pH 2.0, F0721 and IN5.22 showed higher tolerance ability at pH 3.0. F0221 showed the highest bile salt tolerance than other strains at 0.15%, 0.3% and 0.5% bile concentrations, whereas highest sensitivity to the bile stress was found for IN5.22. F0221, IN5.22 and G14 strains showed the highest levels of adherences (range from 106 to 129 cfu/100 cells), and intestine origin bacteria (F0221, F0721 and IN5.22) exhibited higher hydrophobicity values and resistant to phenol ability as compared to the dairy products.
Blocking Effect of HOCl on Adhesion of Marine Microbes
Authors: Liu Huoan , Chen Shuang Kou
Published: September 2011

In this manuscript, we systematically investigated the effect of hypochlorous acid on adhesion of biofouling marine bacteria and diatom utilizing the sea water antifouling electrolysis method. Results showed that when current density was 20 mA/cm2, HClO could block 99.98% of the adhesion of marine bacteria after 3 days, 100% of diatom adhesion after 7 days and 97.28% of diatom adhesion after 70 days of 8h/ day electrolysis, suggesting that hypochlorous acid could effectively block the adhesion of marine bacteria and diatom.
Computational Analysis of Microrna Profiles and their Target Genes Involved in Multidrug Resistance of Breast Cancer Cell
Authors: Chen Guo Qing , Zhao Zhi Wei
Published: September 2011

Multidrug resistance remains a major clinical obstacle to successful treatment of breast cancer and leads to poor prognosis for the patients. Recently studies have shown that microRNAs play an important role in breast cancer cell resistance to chemotherapeutic agents. In this study, microRNA expression profiles of MCF-7/AdrVp and MCF-7 were analyzed using microarray. Gene Ontology and pathways mapping tools were employed to analysis systemically the biological processes and signaling pathways affected by differential expression microRNAs. The results indicated that breast cancer cell resistant to chemotherapy was associated with a group of microRNAs. Gene Ontology and pathway mapping are valid and effective approach to analysis the function of microRNAs and the results could be a guideline for further investigation.
QSAR Studies on some of Dichloroacetyl Oxazolidine Herbicide Safeners
Authors: Zhao Hong Jie , Ye Fei
Published: September 2011

In order to find activity of herbicide safener, The MM+ and Polak-Ribiere algorithm were employed to calculate the properties of dichloroacetyl oxazolidine compounds. The QSAR equation of this series compounds was obtained by using (Least-Squares Regression) PLS regression. The results showed that log p and dipole moment played a major role, variable surface area (aprox), hydration energy, refractivity and polarizability played a secondary role. The equation building of this series compounds can not only forecast the activity of new compound, but also lead design of higher activity herbicide safener.
Novel Approach to Fabricate an Amperometric Biosensor for Glucose Based on Nafion, Azure І and Gold Nanoparticles
Authors: Zhou Yong
Published: September 2011

A novel approach to fabricate an amperometric biosensor for the detection of glucose was described. By means of self-assembled technique and the opposite-charged adsorption, Nafion(Naf), Azure І (Azu), gold nanoparticles (nano-Au) and glucose oxidase (GOD) were immobilized on the gold electrode subsequently. The GOD immobilized on the nano-Au monolayer was stable and retained its functional activity. The stepwise self-assemble procedure of the immunosensor was further characterized by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The factors influencing the performance of the resulting biosensor were studied in detail. The biosensor could detect the glucose in a range of 5.1×10-6~4.0×10-3mol/L.
Improvement of Vindoline Production in a Catharanthus roseus callus Line
Authors: Xiang Bei Bei , Zhu Ye Rong , Wang Wen Juan , Bai Yan Ling , Wang Yong
Published: September 2011

The influence of polyploidity on the accumulation of vindoline in Catharanthus roseus callus cultures was investigated. The callus line (T1) was induced from a tetraploid leaf. Total alkaloids in the callus were extracted and analyzed by LC-MS for qualitification and HPLC for quantification. Results showed that T1 callus cultures could accumulate vindoline to a high level. The highest accumulation of vindoline found in the callus was 0.11 mg g−1 DW. Our results demonstrate that polyploidity could influence the chlorophyll content or chloroplast development and improve vindoline biosynthesis in callus cultures. The T1 callus cultures also accumulated a substance which was not present in the diploid callus. The substance was preliminarily identified by LC-MS as deacetylvindoline, the direct precursor of vindoline biosynthesis. T1 callus could be used for genetic manipulation of the biosynthesis of vinblastine and vincristine, the two important antitumor drugs, and therefore, have potential commercial value.
Analysis for Effect of Dimehypo on Proteolytic Enzyme Activity in the Midgut of the Spider Pardosa pseudoannulata with a Piezoelectric Quartz Crystal Impedance
Authors: Fu Wen Hui , Fang Jun , Wang Zhi
Published: September 2011

A piezoelectric quartz crystal impedance technique has been applied for in situ monitoring of the whole process of the proteolytic enzyme-catalyzed hydrolyzation of casein and the effect of pesticide dimehypo (formula: C5H11NNa2O6S4) on the proteolytic enzyme activity in the midgut of the spider Pardosa pseudoannulata, based on its real-time reponses to the density-viscosity change of the tested solution due to the hydrolyzation occurring on the casein molecules. The results indicated that the hydrolyzation was significantly increased in the presence of low-dose dimehypo, where significantly inhibited in the presence of high-dose dimehypo, and a correlation was found precisely between the enzyme activity reflected by the final frequency shift after the hydrolyzation and the concentration of dimehypo, with a significant frequency response observed even at a 2.88 x 10-4 volume ratio of dimehypo to distilled water. The present work may have presented a novel method feasible for study on the mechanism of excitability of spiders under low-dose dimehypo pesticide and quick detection of proteolytic enzyme activity.
The Study of Influence of Fluorine Ether Acids on Property of Magnetic Fe3O4 Nanoparticles
Authors: Wang Fan , Li De Cai
Published: September 2011

For prepare magnetic liquids with good property, the authors prepared Magnetic Fe3O4 nanoparticles by co-precipitation and magnetic Fe3O4 nanoparticles were modified by the surfactant. Analyzed the product of Magnetic Fe3O4 nanoparticles by X-ray Diffraction Analysis, Transmission Electron Scanning, Saturation Magnetization Test and the result indicate that Magnetic Fe3O4 nanoparticles modified by the surfactant have good dispersity, but it’s saturation magnetization is lower than Magnetic Fe3O4 nanoparticles which notmodified by the surfactant.
The Effect of Asparagus officinalis Polysaccharide on Erythrocyte of S180 Mice Played in Immunological Reaction of Lymphocytes
Authors: Ji Chen Feng , Ji Yu Bin , Min Yong Cui
Published: September 2011

To study Asparagus officinalis polysaccharide on the function of erythrocyte in S180 mice on immunological reaction of lymphocyte. Suspensions of lymphocytes and autologous plasma were separated from whole blood of healthy mice. The erythrocytes were separated from whole blood of Asparagus officinalis polysaccharide mice. Using the autologous plasm as reactive medium, the role of erythrocytes in regulating the immunological reaction of lymphocytes was mesured. The expression of CD25 on lymphocytes was measured by flow cytometry. The immunological regulating ability of erythrocyte in control group is much lower than normal group, and in Asparagus officinalis polysaccharide group is much higher than control group. According to the effects of erythrocyte CD35 on the immune-response of lymphocyte and the different of the expression of CD25 on lymphocytes, we prove that Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice.
Study on Accurate Strength Analysis of Blade Wheel in Vehicular Hydraulic Retarder Based on Sequential Coupling Method
Authors: Wei Wei , Zou Bo , Yan Qing Dong , Li Hui Yuan
Published: September 2011

Based on theory of fluid-solid interaction (FSI), a three dimensional finite element computational model of blade wheel in a vehicular hydraulic retarder was built considering accurate fluid pressure and centrifugal stress with unidirectional FSI method, and viscous numerical simulation of computational fluid dynamics and then strength analysis were implemented, and the reliability of blade wheel was verified. The results showed that incipient fault of safety existed in the root of rotor blade, and the method of accurate strength analysis was credible and facilitate on the platform of commercial code of ANSYS/Workbench.
Digital Oil Depot Designing and the Research of Visual Interactive Technology
Authors: Gao Ying , Zhang Jin Hui , Ji Wei Jun , Guo Shu Xia
Published: September 2011

According to situation of the digital oil depot building and the needs of the current fuel guaranteeing, it makes use of virtual reality technology and three-dimensional visualization technology to build a realist and operating easily digital depot roaming platform. It analysis of the functions and features of each module of the “digital oil depot roaming system” and implements the scene. What’s more, it explores the viewpoint of the system management and collision detection technology and uses DI-Guy software to analysis detailed the simulation of virtual characters. Through modifying character models and creating new actions, it implements the combination of virtual characters and three-dimensional scene with programming by comparing three methods, as well as the realization of the visual interactive electronic technology manual of the digital oil depot system.
Stability Analysis for the Milling of Lateral Walls
Authors: Jiang Ai Jun , Wu Kai
Published: September 2011

The stability model was established and the two-dimensional and three-dimensional lobes were plotted for the milling of lateral walls in order to avoid chatter occurring in the milling of lateral walls. The combination of milling parameters of free chatter can be selected in the stable zone of stability lobes. The stability of milling lateral walls influenced by radial depth of cut and teeth number of the milling cutter was analyzed. It is shown that the stable zone of stability lobes expands gradually as the spindle speed increases. The stable zone become wider as the radial depth of cut increases. The axial depth of cut limits is increased and higher productivity can be obtained as the teeth of the milling cutter decreases.
Wavelet Grey Moment Vector and Hidden Markov Model Based Fault Diagnosis for Ball Bearing
Authors: Xuan Jian Ping , Xu Zeng Bing , Wu Bo , Shi Tie Lin
Published: September 2011

The paper introduces a new approach to detect the fault of bearing based on wavelet grey moment vector and hidden Markov modeling (HMM). Because of non-stationary characteristics of vibration signals of faulty bearings, we propose a new method to extract the wavelet grey moment vectors from these signals. The grey moment vectors are used as feature parameters to train HMMs to establish the database. Fault modes of bearings can be identified by select the HMM with the highest probability. The experimental results show that the proposed approach is effective and accurate to detect the faulty bearing for every single fault.
Size Effect of Waste Compact Disc Shred on Properties of Concrete
Authors: Tang Wai Ching , Lo Yiu , Cui Hong Zhi
Published: September 2011

In this research, the mechanical and fracture behaviours of concrete containing waste CD shreds were investigated using the three-point bending notched beam test, according to RILEM recommendations. The size effect of waste CD shred on concrete properties was the focus of this research. The study indicates that the fracture energy and modified characteristic length were found to increase significantly with increasing the size and volume fraction of CD shreds due to anchoring and bridging effects. In other words, concrete with higher amounts and larger sizes of CD shreds exhibit higher cracking resistance and the brittleness decreases accordingly. However, the strength properties were found to decrease when concrete with larger portion and size of CD shreds.
Scheduling Flexible Manufacturing Systems with Petri Nets Based on the Cell Enumeration Method
Authors: Gu Shen Shen
Published: September 2011

In the field of modern manufacturing, flexible manufacturing systems (FMS) is very important because it can scheduleand optimize multipurpose machines to produce multiple types of products. When applying the FMS technology, Petri Net is used to model the machines, parts and the whole manufacturing progress. The core concern of FMS is to make sure that the manufacturing system can transfer from the original state to the final state, which is called reachabilty. Therefore, reachability analysis is one of the most important problems of FMS. When Petri Net is acyclic, the reachability analysis can be performed by finding a integer solution to a set of linear equation, named fundamental equation, which is known to be NP-complete. In this paper, a novel approach for finding the integer solution is applied by adopting a revised version of the cell enumeration method for an arrangement of hyperplanes in discrete geometry to identify firing count vector solution(s) to the fundamental equation on a bounded integer set with a complexity bound of O((nu)n¡m),where n is the number of nodes, m is the number of arcs and u is the upper bound of the number of firings for all individual arcs.
Scheme Comparison on Unsymmetrical Loading Tunnel Location near Tunnel Portal
Authors: Yu Shu Qiang , Cheng Xu Dong , Qin Peng Ju
Published: September 2011

In the process of tunnel excavation, due to the complexity of geological and geomorphic conditions etc, in the given tunnel heading shape conditions, it is more important to determine the tunnel location near tunnel portal. The large-scale finite numerical simulation software ANSYS is adopted to analyze that stress and displacement of tunnel surrounding rock vary with unsymmetrical location, including burial depth, slope angle and thickness of overburden layer. The analysis results show that: Under the condition of reinforcement in advance, it is proper for the tunnel excavation to reduce burial depth and thickness of overburden layer. This paper provides the theory basis for rational selection of support types and parameters and proper determination of tunnel location.
Research on Testing Method of Dynamic Characteristic for MEMS-Gyroscope
Authors: Zhang Nuer , Ren Yong Feng , Li Sheng Kun
Published: September 2011

Gave a test method of the dynamic characteristics about gyroscope. In the process of using Gyroscope, it will be affected by various external factors irresistibly in making the gyroscope working at certain vibration frequency. Designed the experiments of steel plate vibrating to examine the relationship between the sensitive angular rate of gyroscope and vibration frequency. Firstly, fixed the plate on the desk; then made the plate to vibrate, collected the data from the gyroscope which vibrating with steel plate. To analyze the data of collection, gained the dynamic characteristics of gyroscope. In addition, designed rotating floor calibration experiment, calibrated the relationship between input and output of gyroscope and verified the rationality of the steel plate vibrating experiment. The result prove that gain the dynamic characteristics of gyroscope by steel plate vibrating, not only the method is simple but the result also can achieve the good precision.
Signal Decomposition for Three-Axis Gyroscope
Authors: Wang Hong Wei , Qi Chen Jie , Wu Qiu Xin , Surong Qi Mu , Xing Zhen Hua
Published: September 2011

Study Algorithm of roll, pitch and yaw angular velocity for three-axis gyroscope. the gyro have only one sensitive quality, can sensitize the three axial angular velocity of the rotating carrier at the same time. in the case of gyro’s spin angular velocity presence and pitch or yaw angular velocity worked, gyro output signal contain spin, pitch and yaw angular velocity. Through Uniaxial Gravity Accelerometer’s application, the roll, pitch and yaw angular velocity for three-axis gyroscope can be demodulated.
Research on Screw Thread Vision Measurement
Authors: Jiang Jian Feng , Jiang Zhao Xi , Zhang Qing Chun , Chai Guo Zhong , Lu Cong Da
Published: September 2011

Screw thread, as the most basic and widely used mechanic element, has many traditional methods to verify its dimension consistency. Machine vision technology is gradually applied to inspect screw thread in fastener industry because of its fast, precise and repeatable features. Based on analysis of the features and problems of previous vision measurement methods, this paper provides calculation, analysis and simulation of screw thread’s 3D mathematic model, and finally provides corresponding algorithm to correct calculation error resulting from shading effect of backlight projecting.
The Study of Effects Caused by Job Satisfaction on Performance Evaluation of Professional Coaches in China
Authors: Lin Li
Published: September 2011

Our study has conducted an inquiry ,based on the " The Structure scale for Job performance of Chinese professional coaches" and "Job satisfaction scale ", and 213 coaches from 19 provinces in China were involved in our investigation.The gathered evidence demonstrate the results that job satisfaction has significant and positive correlation of effects with task performance,contextual performance,as well as development performance,on the contrary, it has shown significant positive effects on counter-production performance.
Design and Realization of the Dynamic Magnetic Measurement System for Soft Magnetic Material
Authors: Zheng Liang , Ying Zhi Hua , Kong Wei Peng , Sun Feng , Hu Wei Wei , Xu Jun Ming , Qin Hui Bin
Published: September 2011

A dynamic magnetic measurement system for soft magnetic material based on virtual instrument technology is presented. High frequency AC excitation power supply is adopted to drive soft magnetic material. High speed data transport system composed by FPGA chip ALTERA EP1C12Q240C8N and USB microcontroller Cypress CY7C68013A. The problem of phase-shift of hardware integrator which is difficult to control is solved by digital integration. LabVIEW with powerful data processing capabilities is used to simplifies the hardware structure and improve the testing accuracy and automation level of measurement. The paper also points out problems of digital integration and gives a solution.
Test System for Pressure Pulsation in Centrifugal Pump Based on Virtual Instrument Technology
Authors: Luo Yin , Tang Yue , Yuan Shou Qi , Wang Hui
Published: September 2011

A system based on virtual instrument was set up to test and analyze the pressure pulsation in centrifugal pump. It had adjustable sampling time length and frequency which can avoid the disadvantages of data loss and redundancy caused by fixed sampling parameters. A signal preprocessing toolkit compiled by LabVIEW was set up, which can anti the aliasing of the signal by use of the low pass filter, decrease the noise in the concerned frequency by the wavelet de-noising and efficiently reduce the error caused by test system. Meanwhile, the signal’s frequency characteristic could be analyzed in this system automatic, and the detail of the frequency characteristic could be captured by zoom FFT. All of the toolkits could be used as an automatic real-time system. The feasibility of this system has already been evaluated in a centrifugal pump system, and it is testified that this system can realize and analyze the pressure pulsation automatically.
The Performance Test for Trigger Switch of Underwater Plasma Acoustic Source
Authors: Huang Hai , Lei Kai Zhuo , Huang Jian Guo , Zhang Ling Ling , Qu Jian Kang
Published: September 2011

Underwater plasma acoustic source has many advantages and wide applications, but its properties are greatly affected by the trigger switch. In this paper, the working principles of two types spark gap switch (Fig.1) were analyzed. Four aspects of the performance parameter including switching delay, delay jitter, voltage-drop percentage and transmission efficiency of the trigger switches (see Table 1) were researched by test and measurement. Experimental results show that: in normal condition, with the increase of charging voltage, switching delay time tdand delay time jitter for both spark gap switches will decrease. Meanwhile, the switching delay and delay jitter of type A were always greater than type B; but, the transmission efficiency of type A was 20% higher than type B. The result will guide the experiment of underwater plasma acoustic source and the design of new trigger switch.