bluuebunny/crossref_metadata_2025 huggingface dataset:
STC3141 improves acute lung injury through neutralizing circulating histone in rat with experimentally-induced acute respiratory distress syndrome
Authors: Ge Yangyang , Wang Chenchen , Yao Chenye , Wang Yong , Zheng Yuduo , Luo Junjie , Chen Jiayi , Wang Yu , Wang Fuquan , Wang Li , Lin Yun , Shi Lin , Yao Shanglong
Published: May 2023

Background: Acute respiratory distress syndrome (ARDS) remains a challenge because of its high morbidity and mortality. Circulation histones levels in ARDS patients were correlated to disease severity and mortality. This study examined the impact of histone neutralization in a rat model of acute lung injury (ALI) induced by a lipopolysaccharide (LPS) double-hit.

Methods: Sixty-eight male Sprague-Dawley rats were randomized to sham (N = 8, received saline only) or LPS (N = 60). The LPS double-hit consisted of a 0.8 mg/kg intraperitoneal injection followed after 16 h by 5 mg/kg intra-tracheal nebulized LPS. The LPS group was then randomized into five groups: LPS only; LPS +5, 25, or 100 mg/kg intravenous STC3141 every 8 h (LPS + L, LPS + M, LPS + H, respectively); or LPS + intraperitoneal dexamethasone 2.5 mg/kg every 24 h for 56 h (LPS + D). The animals were observed for 72 h.

Results: LPS animals developed ALI as suggested by lower oxygenation, lung edema formation, and histological changes compared to the sham animals. Compared to the LPS group, LPS + H and +D groups had significantly lower circulating histone levels and lung wet-to-dry ratio, and the LPS + D group also had lower BALF histone concentrations; the blood neutrophils and platelets counts in LPS + D group did not change, meanwhile, the LPS + L, +M and +H groups had significantly lower neutrophil counts and higher platelet counts in the blood; the total number of BALF WBC, platelet counts, MPO and H3 were significantly lower in the LPS + L, +M, +H and +D groups than in the LPS only group; and the degree of inflammation was significantly less in the LPS + L, +M, +H and +D groups, moreover, inflammation in the LPS + L, +M and +H animals showed a dose-dependent response; finally, the LPS + L, +M, +H and +D groups had improved oxygenation compared to the LPS group, and there were no statistical differences in PCO2 or pH among groups. All animals survived.

Conclusion: Neutralization of histone using STC3141, especially at high dose, had similar therapeutic effects to dexamethasone in this LPS double-hit rat ALI model, with significantly decreased circulating histone concentration, improved acute lung injury and oxygenation.
Cardioprotective effect of curcumin on myocardial ischemia/reperfusion injury: a meta-analysis of preclinical animal studies
Authors: Zeng Yi-Fan , Guo Qi-Hao , Wei Xin-Yu , Chen Si-Yu , Deng Sheng , Liu Ji-Jia , Yin Ni , Liu Yan , Zeng Wen-Jing
Published: May 2023

Objective: This meta-analysis aimed to determine the efficacy of curcumin in preventing myocardial ischemia/reperfusion (I/R) injury in animal models.

Methods: Studies published from inception to January 2023 were systematically searched in databases including PubMed, Web of Science, Embase, China’s National Knowledge Infrastructure (CNKI), Wan-Fang database, and VIP database (VIP). The SYRCLE’s RoB tool was used to determine methodological quality. Sensitivity analysis and subgroup analysis were performed when there was high heterogeneity. Publication bias was assessed using a funnel plot.

Results: Thirty-seven studies involving 771 animals were included in this meta-analysis with methodology quality scores ranging from 4 to 7. The results indicated that curcumin treatment significantly improved myocardial infarction size standard mean difference (SMD) = −5.65; 95% confidence interval (CI): 6.94, −4.36; p < 0.01; I2 = 90%). The sensitivity analysis for infarct size showed that the results were stable and reliable. However, the funnel plot was asymmetric. The subgroup analysis included species, animal model, dose, administration, and duration. The results showed that the subgroup dose was statistically significant between subgroups. In addition, curcumin treatment improved cardiac function, myocardial injury enzymes, and oxidative stress levels in animal models of myocardial I/R injury. The funnel plot revealed that there is publication bias for creatine kinase and lactate dehydrogenase. Finally, we performed a meta-analysis of inflammatory cytokines and apoptosis index. The results showed that curcumin treatment downregulated serum inflammatory cytokine levels and myocardial apoptosis index.

Conclusion: This meta-analysis suggests that curcumin has excellent potential for the treatment of myocardial I/R injury in animal models. However, this conclusion needs to be further discussed and verified in large animal models and human clinical trials.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/, identifier CRD42022383901.
Alterations in the gut microbiota and serum metabolomics of spontaneous cholestasis caused by loss of FXR signal in mice
Authors: Wei Shizhang , He Tingting , Zhao Xu , Jing Manyi , Li Haotian , Chen Lisheng , Zheng Ruimao , Zhao Yanling
Published: May 2023

Background: Farnesoid X receptor (FXR) is a key metabolic target of bile acids (BAs) and is also a target for drugs against several liver diseases. However, the contribution of FXR in the pathogenesis of cholestasis is still not fully understood. The purpose of this study is to provide a comprehensive insight into the metabolic properties of FXR-involved cholestasis in mice.

Materials and methods: In this study, an alpha-naphthylisothiocyanate (ANIT)-induced cholestasis mouse model and FXR−/− mice were established to investigate the effect of FXR on cholestasis. The effect of FXR on liver and ileal pathology was evaluated. Simultaneously, Untargeted metabolomics combined with 16s rRNA gene sequencing analysis was applied to reveal the involvement of FXR in the pathogenesis of cholestasis.

Results: The results showed that ANIT (75 mg/kg) induced marked cholestasis in WT and FXR −/− mice. It is noteworthy that FXR−/− mice developed spontaneous cholestasis. Compared with WT mice, significant liver and ileal tissue damage were found. In addition, 16s rRNA gene sequencing analysis revealed gut microbiota dysbiosis in FXR−/− mice and ANIT-induced cholestasis mice. Differential biomarkers associated with the pathogenesis of cholestasis caused by FXR knockout were screened using untargeted metabolomics. Notably, Lactobacillus_ johnsonii_FI9785 has a high correlation with the differential biomarkers associated with the pathogenesis and progression of cholestasis caused by FXR knockout.

Conclusion: Our results implied that the disorder of the intestinal flora caused by FXR knockout can also interfere with the metabolism. This study provides novel insights into the FXR-related mechanisms of cholestasis.
Exercise-induced angiogenesis is attenuated by captopril but maintained under perindopril treatment in hypertensive rats
Authors: Macedo Anderson G. , Miotto Danyelle S. , Tardelli Lidieli P. , Santos Carlos F. , Amaral Sandra L.
Published: May 2023

Angiogenesis is an important exercise-induced response to improve blood flow and decrease vascular resistance in spontaneously hypertensive rats (SHR), but some antihypertensive drugs attenuate this effect. This study compared the effects of captopril and perindopril on exercise-induced cardiac and skeletal muscle angiogenesis. Forty-eight Wistar rats and 48 SHR underwent 60 days of aerobic training or were kept sedentary. During the last 45 days, rats were treated with captopril, perindopril or water (Control). Blood pressure (BP) measurements were taken and histological samples from the tibialis anterior (TA) and left ventricle (LV) muscles were analyzed for capillary density (CD) and vascular endothelial growth factor (VEGF), VEGF receptor-2 (VEGFR-2) and endothelial nitric oxide synthase (eNOS) protein level. Exercise increased vessel density in Wistar rats due to higher VEGFR-2 (+17%) and eNOS (+31%) protein level. Captopril and perindopril attenuated exercise-induced angiogenesis in Wistar rats, but the attenuation was small in the perindopril group, and this response was mediated by higher eNOS levels in the Per group compared to the Cap group. Exercise increased myocardial CD in Wistar rats in all groups and treatment did not attenuate it. Both exercise and pharmacological treatment reduced BP of SHR similarly. Rarefaction was found in TA of SHR compared to Wistar, due to lower levels of VEGF (−26%) and eNOS (−27%) and treatment did not avoid this response. Exercise prevented these reductions in control SHR. While rats treated with perindopril showed angiogenesis in the TA muscle after training, those rats treated with captopril showed attenuated angiogenesis (−18%). This response was also mediated by lower eNOS levels in Cap group compared with Per and control group. Myocardial CD was reduced in all sedentary hypertensive compared with Wistar and training restored the number of vessels compared with sedentary SHR. In conclusion, taken into account only the aspect of vessel growth, since both pharmacological treatments reduced BP in SHR, the result of the present study suggests that perindopril could be a drug of choice over captopril for hypertensive practitioners of aerobic physical exercises, especially considering that it does not attenuate angiogenesis induced by aerobic physical training in skeletal and cardiac muscles.
Flower-inducing technology facilitates speed breeding in cassava
Authors: Rodrmguez Erika Paola Barinas , Morante Nelson , Salazar Sandra , Hyde Peter T. , Setter Tim L. , Kulakow Peter , Aparicio Johan Steven , Zhang Xiaofei
Published: May 2023

Cassava is a tropical crop that provides daily carbohydrates to more than 800 million people. New cassava cultivars with improved yield, disease resistance, and food quality are critical to end hunger and reduce poverty in the tropics. However, the progress of new cultivar development has been dragged down by difficulties obtaining flowers from desired parental plants to enable designed crosses. Inducing early flowering and increasing seed production are crucial to improving the efficiency of developing farmer-preferred cultivars. In the present study, we used breeding progenitors to evaluate the effectiveness of flower-inducing technology, including photoperiod extension, pruning, and plant growth regulators. Photoperiod extension significantly reduced the time to flowering in all 150 breeding progenitors, especially late-flowering progenitors which were reduced from 6-7 months to 3-4 months. Seed production was increased by using the combination of pruning and plant growth regulators. Combining photoperiod extension with pruning and the PGR 6-benzyladenine (synthetic cytokinin) produced significantly more fruits and seeds than only photoperiod extension and pruning. Another growth regulator, silver thiosulfate, commonly used to block the action of ethylene, did not show a significant effect on fruit or seed production when combined with pruning. The present study validated a protocol for flower induction in cassava breeding programs and discussed factors to consider in implementing the technology. By inducing early flowering and increasing seed production, the protocol helped move one step further for speed breeding in cassava.
Hotspots, trends, and advice: a 10-year visualization-based analysis of painting therapy from a scientometric perspective
Authors: Liang Qianrong , Ye Jiarong , Lu Yingyin , Dong Junjie , Shen Heyong , Qiu Hongzhong
Published: May 2023

Purpose

Research on painting therapy is available worldwide and painting therapy is widely applied as a psychological therapy in different fields with diverse clients. As an evidence-based psychotherapy, previous studies have revealed that painting therapy has favorable therapeutic effects. However, limited studies on painting therapy used universal data to assemble in-depth evidence to propose a better recommendation on it for the future use. Large-scale retrospective studies that used bibliometric methodology are lacking. Therefore, this study presented a broad view of painting therapy and provided an intensively analytical insight into the structure of knowledge regarding painting therapy employing bibliometric analysis of articles. CiteSpace software was used to evaluate scientific research on painting therapy globally published from January 2011 to July 2022.

Methods

Publications related to painting therapy from 2011 to 2022 were searched using the Web of Science database. This study employed bibliometric techniques to perform co-citation analysis of authors, visualize collaborations between countries/regions as network maps, and analyze keywords and subjects relevant to painting therapy by using CiteSpace software.

Results

In total, 871 articles met the inclusion criteria. We found that the number of painting therapy publications generally trended incrementally. The United States and United Kingdom made the most contributions to painting therapy research and had the greatest impact on the practical application in other countries. Arts in Psychotherapy and Frontiers in Psychology occupied key publishing positions in this research field. The application groups were mainly children, adolescents, and females, and Western countries paid high attention to painting therapy. The main areas of application of painting therapy were Alzheimer’s disease and other psychosomatic disease fields. Identified research priorities for painting therapy were emotion regulation and mood disorder treatment, personality disorder treatment, personal self-esteem enhancement, and medical humanistic care. Three keywords, “depression,” “women,” and “recovery,” had the strongest citation bursts, which emphasized the research trends.

Conclusion

The general trend for painting therapy research is positive. Our findings provide useful information for researchers on painting therapy to determine new directions in relate to popular issues, collaborators, and research frontiers. Painting therapy holds a promising future, and further studies could explore the clinical implications of this therapy in terms of mechanisms and criteria for assessing efficacy.
The good, the bad, and the ugly: a comprehensive study of temperament and personality traits as correlates of self-reported disruptive behavior problems in male and female adolescents
Authors: Muris Peter , Bakker Ireen , Peulen Myrthe , van Mulekom Sanne , Meesters Cor
Published: May 2023

Purpose

The aim of the present study was to provide a comprehensive picture of temperament and personality traits as correlates of self-reported disruptive behavior problems in male and female adolescents.

Methods

Two-hundred-and-sixty-three non-clinical adolescents aged 12–18 years completed a survey containing standardized scales to measure the HEXACO personality traits, impulsivity, effortful control, Dark Triad traits, and symptoms of oppositional-defiant disorder (ODD) and conduct disorder (CD).

Results

The results showed that good traits (in particular, honesty-humility, agreeableness, and the regulative trait of effortful control) were negatively associated, while bad and ugly traits (especially impulsivity and the Dark Triad traits of psychopathy and Machiavellianism) were positively associated with symptoms of ODD and CD. In addition, regression analyses indicated that both types of disruptive behavior problems were associated with a unique set of temperament and personality correlates, and that specific correlates also differed for both genders.

Conclusions

It is concluded that research on the role of temperament and personality should adopt a broad perspective, taking good, bad, and ugly traits from various theoretical models as well as gender differences into account.
Did China's dual-credit policy promote innovation in new energy vehicle enterprises? An empirical study based on the PSM-DID method
Authors: Sun Jianguo , Tian Mingfu
Published: May 2023

Introduction

Based on the microdata of 36 A-share new energy vehicle (NEV) enterprises from 2015 to 2021, this study empirically investigates whether the introduction of the double credit policy (DCP) promoted the innovation of NEV enterprises.

Methods

The propensity score matching difference-in-differences (PSM-DID) method was used.

Results

The following results were found. First, the introduction of the DCP inhibits the innovation of NEV enterprises. However, as the DCP stabilizes, the inhibitory effect gradually decreases and shows a tendency to turn into a facilitating effect. Second, the DCP affects the innovation output of enterprises by influencing their research and development (R&D) investment. R&D investment has a mediating role in the DCP, affecting the innovation of new energy vehicle enterprises. Third, at the firm level, the inhibitory effect of the DCP is more evident in non-state-owned enterprises (non-SOE) and insignificant for state-owned enterprises (SOE), while at the regional level, the inhibitory effect of the DCP is more evident for non-eastern regions and insignificant for eastern regions.

Discussion

This study finds the inhibitory effect of the implementation of the DCP on the innovation of NEV enterprises and have potential guiding significance for the future formulation of NEV market development policies and the promotion of high-quality development of the NEV industry.
Wastewater monitoring of SARS-CoV-2 in on-grid, partially and fully off-grid Bedouin communities in Southern Israel
Authors: Yaniv Karin , Craddock Hillary A. , Mahameed Fareed , Shagan Marilou , Salah Ikram , Lakkakula Satish , Resnick Keren , Haber Corinne , Davidovitch Nadav , Moran-Gilad Jacob , Kushmaro Ariel , Lipchin Clive
Published: May 2023

Background

Wastewater based epidemiology (WBE) has become an important tool in SARS-CoV-2 surveillance and epidemiology. While WBE measurements generally correlate with observed case numbers in large municipal areas on sewer grids, there are few studies on its utility in communities that are off-grid (non-sewered).

Methods and materials

To explore the applicability of wastewater surveillance in our region, five Bedouin communities along the Hebron Stream in Southern Israel (Negev desert) were sampled. One point (El-Sayed) represents a community with partial connection to the sewer grid system and another point (Um Batin) represents a community with no access to the sewer grid system. The towns of Hura, Lakia, and Tel Al-Sabi/Tel Sheva were on-grid. A total of 87 samples were collected between August 2020 to January 2021 using both grab and composite sampling. RNA was extracted from the raw sewage and concentrated sewage. RT-qPCR was carried out with N1, N2, and N3 gene targets, and findings were compared to human case data from the Israeli Ministry of Health.

Results

SARS-CoV-2 was detected consistently over time in on-grid Bedouin towns (Lakia, Tel Sheva/Tel as-Sabi, and Hura) and inconsistently in smaller, off-grid communities (El-Sayed and Um Batin). The trend in maximum copy number/L appears to be driven by population size. When comparing case numbers normalized to population size, the amount of gene copies/L was inconsistently related to reported case numbers. SARS-CoV-2 was also detected from sewage-impacted environmental waters representing communities with no access to the wastewater grid. When grab sampling and composite sampling data were compared, results were generally comparable however composite sampling produced superior results.

Conclusions

The mismatch observed between detected virus and reported cases could indicate asymptomatic or “silent” community transmission, under-testing within these communities (due to factors like mistrust in government, stigma, misinformation) or a combination therein. While the exact reason for the mismatch between environmental SARS-CoV-2 signals and case numbers remains unresolved, these findings suggest that sewage surveillance, including grab sampling methodologies, can be a critical aspect of outbreak surveillance and control in areas with insufficient human testing and off-grid communities.
Use of the superficial inferior epigastric vein in breast reconstruction with a deep inferior epigastric artery perforator flap
Authors: Varnava Charalampos , Wiebringhaus Philipp , Kampshoff David , Hirsch Tobias , Kueckelhaus Maximilian
Published: May 2023

Background

Autologous breast reconstruction is highly regarded in reconstructive surgery after mastectomy. DIEP flap reconstruction represents the gold standard for autologous breast reconstruction. The major advantages of DIEP flap reconstruction are its adequate volume, large vascular caliber and pedicle length. Despite reliable anatomy, there are procedures where the plastic surgeon's creativity is required, not only to shape the new breast, but also to overcome microsurgical challenges. An important tool in these cases is the superficial epigastric vein (SIEV).

Methods

150 DIEP flap procedures performed between 2018 and 2021 were retrospectively evaluated for SIEV use. Intraoperative and postoperative data were analyzed. Rate of anastomosis revision, total and partial flap loss, fat necrosis and donor site complications were evaluated.

Results

In a total of 150 breast reconstructions with a DIEP flap performed in our clinic, the SIEV was used in 5 cases. The indication for using the SIEV was to improve the venous drainage of the flap or as a graft to reconstruct the main artery perforator. Among the 5 cases, no flap loss occurred.

Conclusions

Use of the SIEV is an excellent method to expand the microsurgical options in breast reconstruction with DIEP flap surgery. It provides a safe and reliable procedure to improve venous outflow in cases of inadequate outflow from the deep venous system. The SIEV could also provide a very good option for fast and reliable application as an interposition device in case of arterial complications.
Knowledge, Attitude and Practices Towards Dengue Fever Among Slum Dwellers: A Case Study in Dhaka City, Bangladesh
Authors: Rahman Md. Mostafizur , Tanni Kamrun Nahar , Roy Tuly , Islam Md. Rakibul , Al Raji Rumi Md. Alim , Sadman Sakib Mohammed , Abdul Quader Masrur , Bhuiyan Nafee-Ul-Islam , Shobuj Ifta Alam , Sayara Rahman Afra , Haque Md. Iftekharul , Faruk Fariha , Tahsan Fahim , Rahman Farzana , Alam Edris , Md. Towfiqul Islam Abu Reza
Published: May 2023

Objectives: This study intends to evaluate Dhaka city slum dwellers’ responses to Dengue fever (DF).

Methods: 745 individuals participated in a KAP survey that was pre-tested. Face-to-face interviews were performed to obtain data. Python with RStudio was used for data management and analysis. The multiple regression models were applied when applicable.

Results: 50% of respondents were aware of the deadly effects of DF, its common symptoms, and its infectious nature. However, many were unaware that DF could be asymptomatic, a previously infected person could have DF again, and the virus could be passed to a fetus. Individuals agreed that their families, communities, and authorities should monitor and maintain their environment to prevent Aedes mosquito breeding. However, overall 60% of the study group had inadequate preventative measures. Many participants lacked necessary practices such as taking additional measures (cleaning and covering the water storage) and monitoring potential breeding places. Education and types of media for DF information were shown to promote DF prevention practices.

Conclusion: Slum dwellers lack awareness and preventative activities that put them at risk for DF. Authorities must improve dengue surveillance. The findings suggest efficient knowledge distribution, community stimulation, and ongoing monitoring of preventative efforts to reduce DF. A multidisciplinary approach is needed to alter dwellers’ behavior since DF control can be done by raising the population’s level of life. People and communities must perform competently to eliminate vector breeding sites.
Gender Differences and the Impact of Partnership and Children on Quality of Life During the COVID-19 Pandemic
Authors: Hettich-Damm Nora , Petersen Juila , Zahn Daniela , Baumkoetter Rieke , Wild Philipp S. , Muenzel Thomas , Schuster Andreas K. , Koenig Jochem , Lackner Karl , Pfeiffer Norbert , Beutel Manfred E. , Engwicht Elisabeth
Published: May 2023

Objectives: The COVID-19 pandemic and its protective measures have changed the daily lives of families and may have affected quality of life (QoL). The aim of this study was to analyze gender differences in QoL and to examine individuals living in different partnership and family constellations.

Methods: Data from the Gutenberg COVID-19 cohort study (N = 10,250) with two measurement time points during the pandemic (2020 and 2021) were used. QoL was assessed using the EUROHIS-QOL questionnaire. Descriptive analyses and autoregressive regressions were performed.

Results: Women reported lower QoL than men, and QoL was significantly lower at the second measurement time point in both men and women. Older age, male gender, no migration background, and higher socioeconomic status, as well as partnership and children (especially in men), were protective factors for QoL. Women living with children under 14 and single mothers reported significantly lower QoL.

Conclusion: Partnership and family were protective factors for QoL. However, women with young children and single mothers are vulnerable groups for lower QoL. Support is especially needed for women with young children.
Changing Colorectal Cancer Trends in Asians: Epidemiology and Risk Factors
Authors: Pardamean Carissa Ikka , Sudigyo Digdo , Budiarto Arif , Mahesworo Bharuno , Hidayat Alam Ahmad , Baurley James W. , Pardamean Bens
Published: May 2023

Once an infrequent disease in parts of Asia, the rate of colorectal cancer in recent decades appears to be steadily increasing. Colorectal cancer represents one of the most important causes of cancer mortality worldwide, including in many regions in Asia. Rapid changes in socioeconomic and lifestyle habits have been attributed to the notable increase in the incidence of colorectal cancers in many Asian countries. Through published data from the International Agency for Cancer Research (IARC), we utilized available continuous data to determine which Asian nations had a rise in colorectal cancer rates. We found that East and South East Asian countries had a significant rise in colorectal cancer rates. Subsequently, we summarized here the known genetics and environmental risk factors for colorectal cancer among populations in this region as well as approaches to screening and early detection that have been considered across various countries in the region.
Kluczowe zmiany w samodzielności i ustrojowej niezależności jednostek samorządu terytorialnego kadencji 2018–2023
Authors: Springer Beata
Published: May 2023

The article deals with the most important changes in the independence and systemic autonomy of local self-government units of the 2018–2023 term. Both normative solutions and actual actions and behaviours that have a fundamental impact on the functioning of local self-government will be analysed. For a more complete picture of the current term, it will be necessary to briefly characterise the changes introduced after 2015. It is also important for the overall analysis to look from the perspective of those currently in power and characterise their vision of the state and the role of local government in the political system. The idea of a strong, effective state determines the perception of the role of local government and the relationship at the interface between local government and government administration.
Three-Dimensional Drone Exploration with Saliency Prediction in Real Unknown Environments
Authors: Xie Ming-Ru , Jung Shing-Yun , Chen Kuan-Wen
Published: May 2023

In this paper, we propose a three-dimensional autonomous drone exploration system (ADES) with a lightweight and low-latency saliency prediction model to explore unknown environments. Several studies have applied saliency prediction in drone exploration. However, these studies are not sufficiently mature. For example, the computational complexity and the size of the developed prediction models have not been considered. In addition, some studies have only proposed saliency prediction models without actually applying them to drones. The ADES system proposed in this paper has a small and fast saliency prediction model and uses a novel drone exploration approach based on visual-inertial odometry to solve the practical problems encountered during drone exploration, such as collisions with and the repeated exploration of salient objects. The proposed ADES system performs comparably to the state-of-the-art, multiple-discontinuous-image saliency prediction network TA-MSNet and enables drones to explore unknown environments with high efficiency.
Field–Road Operation Classification of Agricultural Machine GNSS Trajectories Using Spatio-Temporal Neural Network
Authors: Chen Ying , Li Guangyuan , Zhou Kun , Wu Caicong
Published: May 2023

The classification that distinguishes whether machines are driving on roads or working in fields based on their global navigation satellite system (GNSS) trajectories is essential for effective management of cross-regional agricultural machinery services in China. In this paper, a novel field–road classification method utilizing multiple deep neural networks (MultiDNN) is proposed to enhance the accuracy of field and road point classification. The MultiDNN model incorporates a bi-directional long short-term memory network (BiLSTM), a topology adaptive graph convolution network (TAG), and a self-attention network (ATT) to effectively extract spatio-temporal features for field–road classification. The BiLSTM is used to capture temporal relationships along the time axis of a trajectory, providing global contextual information for each point. Then, the TAG network is used to obtain the spatio-temporal relationships between adjacent points in a trajectory, offering local contextual information for each point. Finally, the ATT network assigns varying weights to features to emphasize important characteristics. The performance of the MultiDNN model was evaluated using a wheat harvesting trajectory dataset, and the results showed that it achieved a high degree of accuracy, up to 89.75%, outperforming the best baseline method (GCN) by 2.79%.
Diversity of Summer Weed Communities in Response to Different Plum Orchard Floor Management in-Row
Authors: Lisek Jerzy
Published: May 2023

The effect of five methods of in-row weed management on the species composition and diversity of summer weed communities in a plum orchard was evaluated. Different methods of orchard floor management (OFM) were implemented for seven consecutive years from 2009 to 2015. Festuca rubra L. ssp. rubra–rhizomatous perennial grass was sown as a cover crop in the alleys of the orchard, in the tree planting year. In the seventh year of OFM implementation, the treatments were ranked according to the decreasing value of the Shannon–Wiener floristic diversity index as follows: tillage, post-emergence herbicides spraying, mowing, mulch, and weedy control. The highest value of Simpson dominance index was found in the control treatment. In plots with such treatments as control, mowing, tillage, and mulch, the dominant species was F. rubra. This meant that the rhizomatous cover crop from the alleys penetrated and affected the in-row flora. Vegetation of mulched plots was characterized by low value of density and soil cover. The obtained results indicated that the flora developing in the control, sprayed with post-emergence herbicides, tilled and mowed plots had greater potential to provide ecosystem services, than the flora of mulched plots.
Research and Design of Hybrid Optimized Backpropagation (BP) Neural Network PID Algorithm for Integrated Water and Fertilizer Precision Fertilization Control System for Field Crops
Authors: Zhu Fenglei , Zhang Lixin , Hu Xue , Zhao Jiawei , Meng Zihao , Zheng Yu
Published: May 2023

China’s field crops such as cotton, wheat, and tomato have been produced on a large scale, but their cultivation process still adopts more traditional manual fertilization methods, which makes the use of chemical fertilizers in China high and causes waste of fertilizer resources and ecological environmental damage. To address the above problems, a hybrid optimization of genetic algorithms and particle swarm optimization (GA–PSO) is used to optimize the initial weights of the backpropagation (BP) neural network, and a hybrid optimization-based BP neural network PID controller is designed to realize the accurate control of fertilizer flow in the integrated water and fertilizer precision fertilization control system for field crops. At the same time, the STM32 microcontroller-based precision fertilizer application control system for integrated water and fertilizer application of large field crops was developed and the performance of the controller was verified experimentally. The results show that the controller has an average maximum overshoot of 5.1% and an average adjustment time of 68.99 s, which is better than the PID and PID control algorithms based on BP neural network (BP–PID) controllers; among them, the hybrid optimization of PID control algorithm based on BP neural network by particle swarm optimization and genetic algorithm(GA–PSO–BP–PID) controller has the best-integrated control performance when the fertilizer application flow rate is 0.6m3/h.
Predicting SARS-CoV-2 Variant Using Non-Invasive Hand Odor Analysis: A Pilot Study
Authors: Gokool Vidia A. , Crespo-Cajigas Janet , Ramírez Torres Andrea , Forsythe Liam , Abella Benjamin S. , Holness Howard K. , Johnson Alan T. Charlie , Postrel Richard , Furton Kenneth G.
Published: May 2023

The adaptable nature of the SARS-CoV-2 virus has led to the emergence of multiple viral variants of concern. This research builds upon a previous demonstration of sampling human hand odor to distinguish SARS-CoV-2 infection status in order to incorporate considerations of the disease variants. This study demonstrates the ability of human odor expression to be implemented as a non-invasive medium for the differentiation of SARS-CoV-2 variants. Volatile organic compounds (VOCs) were extracted from SARS-CoV-2-positive samples using solid phase microextraction (SPME) coupled with gas chromatography–mass spectrometry (GC–MS). Sparse partial least squares discriminant analysis (sPLS-DA) modeling revealed that supervised machine learning could be used to predict the variant identity of a sample using VOC expression alone. The class discrimination of Delta and Omicron BA.5 variant samples was performed with 95.2% (±0.4) accuracy. Omicron BA.2 and Omicron BA.5 variants were correctly classified with 78.5% (±0.8) accuracy. Lastly, Delta and Omicron BA.2 samples were assigned with 71.2% (±1.0) accuracy. This work builds upon the framework of non-invasive techniques producing diagnostics through the analysis of human odor expression, all in support of public health monitoring.
Abundance and Distribution of Microplastics in Invertebrate and Fish Species and Sediment Samples along the German Wadden Sea Coastline
Authors: Polt Laura , Motyl Larissa , Fischer Elke Kerstin
Published: May 2023

Monitoring strategies are becoming increasingly important as microplastic contamination increases. To find potentially suitable organisms and sites for biota monitoring in the German Wadden Sea, we collected invertebrates (n = 1585), fish (n = 310), and sediment cores (n = 12) at 10 sites along the coast of Lower Saxony between 2018 and 2020. For sample processing of biota, the soft tissue was digested and the sediment samples additionally underwent a subsequent density separation step. Microplastic particles were identified using Nile red and fluorescence microscopy, followed by polymer composition analysis of a subset of particles via µRaman spectroscopy. All investigated species, sediment cores, and sites contained microplastics, predominantly in the morphology class of fragments. Microplastics were found in 92% of Arenicola marina, 94% of Littorina littorea, 85% of Mytilus edulis, and 79% of Platichthys flesus, ranging from 0 to 248.1 items/g. Sediment core samples contained MPs ranging from 0 to 8128 part/kg dry weight of sediment. In total, eight polymers were identified, predominantly consisting of polyethylene, polyvinylchloride, and polyethylene terephthalate. Considering the sampling, processing, and results, the species Mytilus edulis and Platichthys flesus are suitable species for future microplastic monitoring in biota.
Dietary Fishmeal Can Be Partially Replaced with Non-Grain Compound Proteins through Evaluating the Growth, Biochemical Indexes, and Muscle Quality in Marine Teleost Trachinotus ovatus
Authors: Su Zeliang , Ma Yongcai , Chen Fang , An Wenqiang , Zhang Guanrong , Xu Chao , Xie Dizhi , Wang Shuqi , Li Yuanyou
Published: May 2023

In the context of human food shortages, the incorporation of non-grain feedstuff in fish feed deserves more research attention. Here, the feasibility and appropriate ratio of non-grain compound protein (NGCP, containing bovine bone meal, dephenolized cottonseed protein, and blood cell meal) for dietary fishmeal (FM) replacement were explored in golden pompano (Trachinotus ovatus). Four isonitrogenous (45%) and isolipidic (12%) diets (Control, 25NGP, 50NGP, and 75NGP) were prepared. Control contained 24% FM, whereas the FM content of 25NGP, 50NGP, and 75NGP was 18%, 12%, and 6%, respectively, representing a 25%, 50%, and 75% replacement of FM in Control by NGCP. Juvenile golden pompano (initial weight: 9.71 ± 0.04 g) were fed the four diets for 65 days in sea cages. There was no significant difference between the 25NGP and Control groups in terms of weight gain, weight gain rate, and specific growth rate; contents of crude protein, crude lipid, moisture, and ash in muscle and whole fish; muscle textural properties including hardness, chewiness, gumminess, tenderness, springiness, and cohesiveness; and serum biochemical indexes including total protein, albumin, blood urea nitrogen, HDL cholesterol, total cholesterol, and triglycerides. However, the golden pompano in 50NGP and 75NGP experienced nutritional stress, and thus some indicators were negatively affected. In addition, compared to the Control group, the expression levels of genes related to protein metabolism (mtor, s6k1, and 4e-bp1) and lipid metabolism (pparγ, fas, srebp1, and acc1) of the 25NGP group showed no significant difference, but the 4e-bp1 and pparγ of the 75NGP group were significantly upregulated and downregulated, respectively (p < 0.05), which may explain the decline in fish growth performance and muscle quality after 75% FM was replaced by NGCP. The results suggest that at least 25% FM of Control can be replaced by NGCP, achieving a dietary FM content of as low as 18%; however, the replacement of more than 50% of the dietary FM negatively affects the growth and muscle quality of golden pompano.
Seasonal Variation and Factors Affecting Trypanosoma theileri Infection in Wild Sika Deer (Ezo Sika Deer Cervus nippon yesoensis) in Eastern Hokkaido
Authors: Hong Yujon , Suganuma Keisuke , Ohari Yuma , Kayano Mitsunori , Nakazaki Kenji , Fukumoto Shinya , Kawazu Shin-ichiro , Inoue Noboru
Published: May 2023

Trypanosoma (Megatrypanum) spp. are isolated from domestic and wild ruminants, including deer, worldwide. The prevalence of trypanosomes in mammals is influenced by a number of factors such as host age and vector abundance. However, the seasonal variation of and factors affecting trypanosome infection in the wild deer population remain elusive. In this study, we analyzed the seasonal variation in trypanosome prevalence and the factors that affect Trypanosoma theileri Laveran, 1902, infection in wild sika deer (Ezo sika deer) Cervus nippon yesoensis (Heude, 1884) in Eastern Hokkaido through a two-year survey. Seasonal variation in the prevalence of trypanosome infection in the deer population ranged from 0 to 41% as per hematocrit concentration and 17 to 89% as per PCR results. In general, the prevalence of T. theileri by PCR in 2020 was higher than that in 2019. Moreover, the prevalence was significantly higher in the aged population than among the younger population. These findings may explain why individual conditions and sampling season were associated with trypanosome prevalence. This is the first study to investigate the seasonal variation in and risk factors affecting trypanosome infection in wild deer.
Simultaneous Occurrence of Hypospadias and Bilateral Cleft Lip and Jaw in a Crossbred Calf: Clinical, Computer Tomographic, and Genomic Characterization
Authors: Marc Simona , Mizeranschi Alexandru Eugeniu , Paul Cristina , Otavă Gabriel , Savici Jelena , Sicoe Bogdan , Torda Iuliu , Huțu Ioan , Mircu Călin , Ilie Daniela Elena , Carabaș Mihai , Boldura Oana Maria
Published: May 2023

Congenital abnormalities in animals, including abnormalities of the cleft lip and jaw and hypospadias have been reported in all domesticated species. They are a major concern for breeders due to the increased economic loss they entail. In this article, we described a congenital bilateral cheilognathoschisis (cleft lip and jaw) with campylognathia in association with penile hypospadias and preputial hypoplasia with failure of preputial fusion in a Bos taurus crossbred Piedmontese × Wagyu calf. Clinical examination, computed tomography, and whole genome sequencing were performed to describe and identify a possible cause of the abnormalities. Clinical examination revealed a bilateral cheilognathoschisis of approximately 4 cm in length and 3 cm in width in the widest part, with computer tomography analyses confirming the bilateral absence of the processus nasalis of the incisive bone and the lateral deviation of the processus palatinus towards the left side. Genomic data analyses identified 13 mutations with a high impact on the products of the following overlapped genes: ACVR1, ADGRA2, BHMT2, BMPR1B, CCDC8, CDH1, EGF, F13A1, GSTP1, IRF6, MMP14, MYBPHL, and PHC2 with ADGRA2, EGF, F13A1, GSTP1, and IRF6 having mutations in a homozygous state. The whole genome investigation indicates the involvement of multiple genes in the birth defects observed in this case.
Dynamic Profile of the Yak Mammary Transcriptome during the Lactation Cycle
Authors: Xia Wei , Liu Yili , Loor Juan J. , Bionaz Massimo , Jiang Mingfeng
Published: May 2023

The objective of this study was to assess the transcriptome of the mammary tissue of four yaks during the whole lactation cycle. For this purpose, biopsies of the mammary gland were performed at −30, −15, 1, 15, 30, 60, 120, 180, and 240 days relative to parturition (d). The transcriptome analysis was performed using a commercial bovine microarray platform and the results were analyzed using several bioinformatic tools. The statistical analysis using an overall false discovery rate ≤ 0.05 for the effect of whole lactation and p < 0.05 for each comparison identified >6000 differentially expressed genes (DEGs) throughout lactation, with a large number of DEGs observed at the onset (1 d vs. −15 d) and at the end of lactation (240 d vs. 180 d). Bioinformatics analysis revealed a major role of genes associated with BTA3, BTA4, BTA6, BTA9, BTA14, and BTA28 in lactation. Functional analysis of DEG underlined an overall induction of lipid metabolism, suggesting an increase in triglycerides synthesis, likely regulated by PPAR signaling. The same analysis revealed an induction of amino acid metabolism and secretion of protein, with a concomitant decrease in proteasome, indicating a major role of amino acid handling and reduced protein degradation in the synthesis and secretion of milk proteins. Glycan biosynthesis was induced for both N-glycan and O-glycan, suggesting increased glycan content in the milk. The cell cycle and immune response, especially antigen processing and presentation, were strongly inhibited during lactation, suggesting that morphological changes are minimized during lactation, while the mammary gland prevents immune hyper-response. Transcripts associated with response to radiation and low oxygen were enriched in the down-regulated DEG affected by the stage of lactation. Except for this last finding, the functions affected by the transcriptomic adaptation to lactation in mammary tissue of yak are very similar to those observed in dairy cows.
AgriMFLN: Mixing Features LSTM Networks for Sentiment Analysis of Agricultural Product Reviews
Authors: Liu Runmeng , Wang Haiyan , Li Yuanxi
Published: May 2023

With the rapid development of the Internet, agricultural products have entered e-commerce platforms, and agricultural product reviews have become an important reference for consumers when purchasing agricultural products. However, due to the characteristics of different lengths, rich context-sensitive information, and multi-level information in the sentences of agricultural product reviews, the existing sentiment analysis methods cannot perform well enough to identify the sentiment tendency. To address this issue, we abstract the problem as a binary classification task to extract consumers’ sentiment orientation by proposing a new method. This method utilizes an attention mechanism to assign different weights to different key information in the sentence, thereby extracting abundant semantic information from the sentence. The design of the long short-term memory (LSTM) gate can effectively solve the problem of extracting long sequences and context-related information. The proposed model achieves superior results on two agricultural product datasets compared to other baseline models, providing guidance for merchants to improve agricultural product quality and enhance customer satisfaction.
A High-Sensitivity Magnetic Field Sensor Based on PDMS Flexible Resonator
Authors: Rong Jiamin , Xu Weikang , Xing Enbo , Tang Jun
Published: May 2023

High-sensitivity resonator magnetic sensing requires a significant magnetostrictive response, while the narrow linewidth mode of a high-Q resonator can provide a high-precision frequency resolution. Therefore, a polydimethylsiloxane (PDMS) flexible resonator with both a low Young’s modulus and high optical transmittance is an ideal platform for realizing high-sensitivity magnetic sensing. Based on the sandwich structure of the PDMS flexible resonator, the mechanism of the magnetic field sensitivity of the PDMS flexible resonator sandwich structure is studied, and the impact of changes in the refractive index and radius on the sensor device is analyzed. In order to optimize the sensitivity of the sensor, when an external magnetic field acts on the sandwich structure, the impacts of three aspects on the sensitivity of the sensor are simulated and analyzed: different coupling positions of PDMS flexible resonator, different radii, and PDMS mixing ratios. The trend of sensitivity change is obtained, and the physical explanation of the sensitivity trend is analyzed. By optimizing these three aspects, the magnetic field sensitivity is eventually calculated as 19.02 nm/mT. Based on the existing experimental conditions and the preparation technology of the PDMS flexible resonator, the measured magnetic field sensitivity is 4.23 nm/mT.