bluuebunny/crossref_metadata_2025 huggingface dataset:
Red Imported Fire Ants Reduce Invertebrate Abundance, Richness, and Diversity in Gopher Tortoise Burrows
Authors: Epperson Deborah M. , Allen Craig R. , Hogan Katharine F. E.
Published: December 2020

Gopher Tortoise (Gopherus polyphemus) burrows support diverse commensal invertebrate communities that may be of special conservation interest. We investigated the impact of red imported fire ants (Solenopsis invicta) on the invertebrate burrow community at 10 study sites in southern Mississippi, sampling burrows (1998–2000) before and after bait treatments to reduce fire ant populations. We sampled invertebrates using an ant bait attractant for ants and burrow vacuums for the broader invertebrate community and calculated fire ant abundance, invertebrate abundance, species richness, and species diversity. Fire ant abundance in gopher tortoise burrows was reduced by >98% in treated sites. There was a positive treatment effect on invertebrate abundance, diversity, and species richness from burrow vacuum sampling which was not observed in ant sampling from burrow baits. Management of fire ants around burrows may benefit both threatened gopher tortoises by reducing potential fire ant predation on hatchlings, as well as the diverse burrow invertebrate community. Fire-ant management may also benefit other species utilizing tortoise burrows, such as the endangered Dusky Gopher Frog and Schaus swallowtail butterfly. This has implications for more effective biodiversity conservation via targeted control of the invasive fire ant at gopher tortoise burrows.
Synchronization Dynamics in Non-Normal Networks: The Trade-Off for Optimality
Authors: Muolo Riccardo , Carletti Timoteo , Gleeson James P. , Asllani Malbor
Published: December 2020

Synchronization is an important behavior that characterizes many natural and human made systems that are composed by several interacting units. It can be found in a broad spectrum of applications, ranging from neuroscience to power-grids, to mention a few. Such systems synchronize because of the complex set of coupling they exhibit, with the latter being modeled by complex networks. The dynamical behavior of the system and the topology of the underlying network are strongly intertwined, raising the question of the optimal architecture that makes synchronization robust. The Master Stability Function (MSF) has been proposed and extensively studied as a generic framework for tackling synchronization problems. Using this method, it has been shown that, for a class of models, synchronization in strongly directed networks is robust to external perturbations. Recent findings indicate that many real-world networks are strongly directed, being potential candidates for optimal synchronization. Moreover, many empirical networks are also strongly non-normal. Inspired by this latter fact in this work, we address the role of the non-normality in the synchronization dynamics by pointing out that standard techniques, such as the MSF, may fail to predict the stability of synchronized states. We demonstrate that, due to a transient growth that is induced by the structure’s non-normality, the system might lose synchronization, contrary to the spectral prediction. These results lead to a trade-off between non-normality and directedness that should be properly considered when designing an optimal network, enhancing the robustness of synchronization.
Retrofit Decarbonization of Coal Power Plants—A Case Study for Poland
Authors: Qvist Staffan , Gładysz Paweł , Bartela Łukasz , Sowiżdżał Anna
Published: December 2020

Out of 2 TWe of coal power plant capacity in operation globally today, more than half is less than 14 years old. Climate policy related to limiting CO2-emissions makes the longer-term operation of these plants untenable. In this study, we assess the spectrum of available options for the future of both equipment and jobs in the coal power sector by assessing the full scope of “retrofit decarbonization” options. Retrofit decarbonization is an umbrella term that includes adding carbon capture, fuel conversion, and the replacement of coal boilers with new low-carbon energy sources, in each case re-using as much of the existing equipment as economically practicable while reducing or eliminating emissions. This article explores this idea using the Polish coal power fleet as a case study. Retrofit decarbonization in Poland was shown to be most attractive using high-temperature small modular nuclear reactors (SMRs) to replace coal boilers, which can lower upfront capital costs by ~28–35% and levelized cost of electricity by 9–28% compared to a greenfield installation. If retrofit decarbonization is implemented globally by the late 2020s, up to 200 billion tons of otherwise-committed CO2-emissions could be avoided.
Macroeconomic Electric Energy Production Efficiency of Photovoltaic Panels in Single-Family Homes in Poland
Authors: Bukowski Marcin , Majewski Janusz , Sobolewska Agnieszka
Published: December 2020

An increase in energy demand that is caused by fast economic development, a limited and constantly decreasing supply of traditional energy sources, as well as excessive environmental pollution that is caused by an increasing concentration of dust and gases in the atmosphere constitute the main factors that contribute to the ever-increasing interest in renewable sources of energy. The most important and promising renewable source of energy is thought to be solar energy. The aim of the paper is to assess the macroeconomic investment efficiency of photovoltaic installations in order to meet the demand for electric energy in single-family homes in Polish conditions. The conducted analysis comprises market characteristics and legal regulations concerning the sale of electric energy in Poland. Calculations were made for 320 variants that differed with regard to investment location, building orientation, and roof inclination. The results indicate that the most beneficial region for photovoltaic micro-installations, from a social perspective, is the south-east of and central Poland. The highest values of economic efficiency were achieved in the case of a southern roof inclination as well as a south-eastern and south-western building orientation. No big differences were observed in the economic investment efficiency for the panel inclinations. The calculated Discounted Payback Period, depending on the calculation method, equals 5.4 to 10 years. The results of the study confirm that the implemented support instruments for investments in photovoltaic installations producing energy for single-family house demand is economically viable.
Software Solution for Modeling, Sizing, and Allocation of Active Power Filters in Distribution Networks
Authors: Buła Dawid , Grabowski Dariusz , Lewandowski Michał , Maciążek Marcin , Piwowar Anna
Published: December 2020

The paper is related to the problem of modeling and optimizing power systems supplying, among others, nonlinear loads. A software solution that allows the modeling and simulation of power systems in the frequency domain as well as the sizing and allocation of active power filters has been developed and presented. The basic assumptions for the software development followed by the models of power system components and the optimization assumptions have been described in the paper. On the basis of an example of a low-voltage network, an analysis of the selection of the number and allocation of active power filters was carried out in terms of minimizing losses and investment costs under the assumed conditions for voltage total harmonic distortion (THD) coefficients in the network nodes. The presented examples show that the appropriate software allows for an in-depth analysis of possible solutions and, furthermore, the selection of the optimal one for a specific case, depending on the adopted limitations, expected effects, and investment costs. In addition, a very high computational efficiency of the adopted approach to modeling and simulation has been demonstrated, despite the use of (i) element models for which parameters depend on the operating point (named iterative elements), (ii) active filter models taking into account real harmonics reduction efficiency and power losses, and (iii) a brute force algorithm for optimization.
Shape Influence of Active Material Micro-Structure on Diffusion and Contact Stress in Lithium-Ion Batteries
Authors: Clerici Davide , Mocera Francesco , Somà Aurelio
Published: December 2020

Electrochemical-mechanical modelling is a key issue to estimate the damage of active material, as direct measurements cannot be performed due to the particles nanoscale. The aim of this paper is to overcome the common assumptions of spherical and standalone particle, proposing a general approach that considers a parametrized particle shape and studying its influence on the mechanical stresses which arise in active material particles during battery operation. The shape considered is a set of ellipsoids with variable aspect ratio (elongation), which aims to approximate real active material particles. Active material particle is divided in two domains: non-contact domain and contact domain, whether contact with neighbouring particles affects stress distribution or not. Non-contact areas are affected by diffusion stress, caused by lithium concentration gradient inside particles. Contact areas are affected simultaneously by diffusion stress and contact stress, caused by contact with neighbouring particles as a result of particle expansion due to lithium insertion. A finite element model is developed in Ansys™APDL to perform the multi-physics computation in non-spherical domain. The finite element model is validated in the spherical case by analytical models of diffusion and contact available for simple geometry. Then, the shape factor is derived to describe how particle shape affects mechanical stress in non-contact and contact domains.
Polybenzimidazole-Based Polymer Electrolyte Membranes for High-Temperature Fuel Cells: Current Status and Prospects
Authors: Zhou Zhengping , Zholobko Oksana , Wu Xiang-Fa , Aulich Ted , Thakare Jivan , Hurley John
Published: December 2020

Polymer electrolyte membrane fuel cells (PEMFCs) expect a promising future in addressing the major problems associated with production and consumption of renewable energies and meeting the future societal and environmental needs. Design and fabrication of new proton exchange membranes (PEMs) with high proton conductivity and durability is crucial to overcome the drawbacks of the present PEMs. Acid-doped polybenzimidazoles (PBIs) carry high proton conductivity and long-term thermal, chemical, and structural stabilities are recognized as the suited polymeric materials for next-generation PEMs of high-temperature fuel cells in place of Nafion® membranes. This paper aims to review the recent developments in acid-doped PBI-based PEMs for use in PEMFCs. The structures and proton conductivity of a variety of acid-doped PBI-based PEMs are discussed. More recent development in PBI-based electrospun nanofiber PEMs is also considered. The electrochemical performance of PBI-based PEMs in PEMFCs and new trends in the optimization of acid-doped PBIs are explored.
A Novel Feature Set for Low-Voltage Consumers, Based on the Temporal Dependence of Consumption and Peak Demands
Authors: Claeys Robbert , Azaioud Hakim , Cleenwerck Rémy , Knockaert Jos , Desmet Jan
Published: December 2020

This paper proposes a novel feature construction methodology aiming at both clustering yearly load profiles of low-voltage consumers, as well as investigating the stochastic nature of their peak demands. These load profiles describe the electricity consumption over a one-year period, allowing the study of seasonal dependence. The clustering of load curves has been extensively studied in literature, where clustering of daily or weekly load curves based on temporal features has received the most research attention. The proposed feature construction aims at generating a new set of variables that can be used in machine learning applications, stepping away from traditional, high dimensional, chronological feature sets. This paper presents a novel feature set based on two types of features: respectively the consumption time window on a daily and weekly basis, and the time of occurrence of peak demands. An analytic expression for the load duration curve is validated and leveraged in order to define the the region that has to be considered as peak demand region. The clustering results using the proposed set of features on a dataset of measured Flemish consumers at 15-min resolution are evaluated and interpreted, where special attention is given to the stochastic nature of the peak demands.
SIoT: A New Strategy to Improve the Network Lifetime with an Efficient Search Process
Authors: Zannou Abderrahim , Boulaalam Abdelhak , Nfaoui El Habib
Published: December 2020

The Social Internet of Things (SIoT) means that every node can use a set of nodes that are considered as friends to search for a specific service. However, this is a slow process because each node is required to manage a high number of friends. Thus, the SIoT issue consists of how to select the right friends that improve the network navigability. The enhancement of the network navigability boosts the search for a service to be rapid but not guaranteed. Furthermore, sending requests from the shortest paths involves the rapid search, but the network lifetime can be reduced due to the number of requests that can be transmitted and processed by the nodes that have low power energy. This paper proposes a new approach that improves the network navigability, speeds up the search process, and increases the network lifetime. This approach aims at creating groups dynamically by nodes where each group has a master node, second, using a consensus algorithm between master nodes to agree with a specific capability, finally adopting a friendship selection method to create a social network. Thus, the friends will be sorted periodically for the objective of creating simultaneously a balance between the energy consumption and the rapid search process. Simulation results on the Brightkite location-based online social network dataset demonstrate that our proposal outperforms baseline methods in terms of some parameters of network navigability, path length to reach the providers, and network lifetime.
Soft Selective Sweep on Chemosensory Genes Correlates with Ancestral Preference for Toxic Noni in a Specialist Drosophila Population
Authors: Ferreira Erina A. , Lambert Sophia , Verrier Thibault , Marion-Poll Frédéric , Yassin Amir
Published: December 2020

Understanding how organisms adapt to environmental changes is a major question in evolution and ecology. In particular, the role of ancestral variation in rapid adaptation remains unclear because its trace on genetic variation, known as soft selective sweep, is often hardly recognizable from genome-wide selection scans. Here, we investigate the evolution of chemosensory genes in Drosophila yakuba mayottensis, a specialist subspecies on toxic noni (Morinda citrifolia) fruits on the island of Mayotte. We combine population genomics analyses and behavioral assays to evaluate the level of divergence in chemosensory genes and perception of noni chemicals between specialist and generalist subspecies of D. yakuba. We identify a signal of soft selective sweep on a handful of genes, with the most diverging ones involving a cluster of gustatory receptors expressed in bitter-sensing neurons. Our results highlight the potential role of ancestral genetic variation in promoting host plant specialization in herbivorous insects and identify a number of candidate genes underlying behavioral adaptation.
Life Threatening Reactive Left Ventricular Hypertrophy Due to Increased Blood Flow after Coronary Artery Bypass Grafting
Authors: Balan Robert , Flora Christian , Elsner Dietmar , Massoudy Parwis
Published: December 2020

A 68-year-old patient with triple vessel coronary artery disease was scheduled for elective coronary artery bypass grafting. Three days after the uneventful surgery, the patient went into cardiogenic shock, which was deemed to be caused by a dynamic left ventricular outflow tract obstruction and high-grade mitral regurgitation, which both had not been present before surgery. On an emergency basis, surgical transvalvular septal myectomy and mitral valve replacement were performed. After initial extra corporeal membrane oxygenation (ECMO) therapy and a prolonged (intensive care unit) ICU stay the patient finally recovered and is well one and a half years after surgery.
Adverse Childhood Experiences on Reproductive Plans and Adolescent Pregnancy in the Gulf Resilience on Women’s Health Cohort
Authors: Flaviano Megan , Harville Emily W.
Published: December 2020

We investigated if adverse childhood experiences (ACEs) and ACE sub-types were associated with increased odds of planning to have children and adolescent pregnancy. The Gulf Resilience on Women’s Health (GROWH) is a diverse cohort of reproductive-age women living in southeastern Louisiana during the 2010 Deepwater Horizon oil spill. In our sample of 1482 women, we used multinomial logistic regression to model odds ratios of wanting future children and assessed effect measure modification by educational attainment. We also estimated odds ratios of adolescent pregnancy with binomial logistic regression. Exposure to ACEs increased odds of wanting future children across all ACE sub-types. Among women with lower educational attainment, three or more ACEs (overall, childhood, and adolescence) had over two times the odds of wanting future children. History of ACE and the various sub-types, except for emotional abuse, were associated with increased risk of adolescent pregnancy. ACEs may be linked to adolescent pregnancy and reproductive plans, and variations by educational status highlighted social discrepancies and importance of social context in evaluation and intervention.
Participatory Action Research Applied to an Ear, Nose, and Throat Specialty Service Redesign in Remote Australia: A Mixed-Methods Study of Key Stakeholder Perspectives
Authors: Jacups Susan P. , Kinchin Irina , Edwards Layla
Published: December 2020

This mixed-methods study reports on the key stakeholders’ perspectives on the ear, nose, and throat (ENT) service redesign in remote Australia, using a participatory action research (PAR) approach. A primary health care (PHC) clinician survey was conducted to assess local needs and possible educational gaps in clinical knowledge. This was followed by an internal stakeholder forum and a follow-up survey with Torres and Cape Hospital and Health Service staff to gain their perspectives on current service delivery and table ideas for a new ENT health service model. Qualitative data were analyzed inductively and grouped in emerging themes. Quantitative data were imported into tables and analyzed descriptively. PAR allowed for input from 19 PHC clinicians, 10 face-to-face stakeholders perspectives, and 18 stakeholder follow-up survey respondents. Four themes emerged: 1. Training for health workers in ENT management; 2. Improved local service access; 3. New referral pathways to improve continuity of care; and 4. Introduction of telehealth. PAR engaged key stakeholders, identifying gaps in ENT service delivery, and guided the development of the new service model. The inclusion of stakeholders throughout the service redesign process is likely to create a more sustainable model of care which already has local “buy-in”.
Understanding Challenges to Well-Being among Latina FarmWorkers in Rural Idaho Using in an Interdisciplinary, Mixed-Methods Approach
Authors: Curl Cynthia L. , Meierotto Lisa , Som Castellano Rebecca L.
Published: December 2020

The aim of this study was to identify social, cultural and workplace-related risk factors affecting well-being among Latina farmworkers in rural Idaho. We recruited 70 Latina farmworkers from southwestern Idaho in 2019. We employed an inter-disciplinary, mixed-methods approach—including surveys, focus groups, interviews, and pesticide biomonitoring—to characterize multiple domains that influence well-being, including food security and access, housing conditions, social supports, access to medical care, and workplace safety. Six major themes emerged as primary challenges to Latina farmworkers’ well-being. In the public sphere, study participants identified these challenges as long working hours, concerns regarding pesticide exposure, and lack of enforcement of regulatory protections. Participants’ concerns regarding pesticide exposure were underscored by biological sampling results; multiple biomarkers of pesticide exposure were detected in all samples, with the highest concentrations measured in samples collected from women who reported mixing, loading or applying pesticides. Within the private sphere, food security and provisioning, childcare responsibilities, and social isolation were identified as significant challenges to well-being. Gender, ethnicity, and geography emerged as important, intersecting statuses that shaped the life experiences of these agricultural workers. Our findings suggest that gender may play a particularly critical role in the unique challenges facing Latina farmworkers. As a result, the services and regulations needed to support well-being in this population may be highly specific, and almost certainly include attention to work–family dynamics, pesticide exposure, and social connections.
Inter- and Intra-Day Comparisons of Smartphone-Derived Heart Rate Variability across Resistance Training Overload and Taper Microcycles
Authors: Williams Tyler D. , Esco Michael R. , Fedewa Michael V. , Bishop Phillip A.
Published: December 2020

The purposes of this study were: (1) to determine if smartphone-derived heart rate variability (HRV) could detect changes in training load during an overload microcycle and taper, and (2) to determine the reliability of HRV measured in the morning and measured immediately prior to the testing session. Twelve powerlifters (male = 10, female = 2) completed a 3-week resistance training program consisting of an introduction microcycle, overload microcycle, and taper. Using a validated smartphone application, daily measures of resting, ultra-short natural logarithm of root mean square of successive differences were recorded in the morning (LnRMSSDM) and immediately before the test session (LnRMSSDT) following baseline, post-overload, and post-taper testing. LnRMSSDM decreased from baseline (82.9 ± 13.0) to post-overload (75.0 ± 9.9, p = 0.019), while post-taper LnRMSSDM (81.9 ± 7.1) was not different from post-overload (p = 0.056) or baseline (p = 0.998). No differences in LnRMSSDT (p < 0.05) were observed between baseline (78.3 ± 9.0), post-overload (74.4 ± 10.2), and post-taper (78.3 ± 8.0). LnRMSSDM and LnRMSSDT were strongly correlated at baseline (ICC = 0.71, p < 0.001) and post-overload (ICC = 0.65, p = 0.010), whereas there was no relationship at post-taper (ICC = 0.44, p = 0.054). Bland–Altman analyses suggest extremely wide limits of agreement (Bias ± 1.96 SD) between LnRMSSDM and LnRMSSDT at baseline (4.7 ± 15.2), post-overload (0.5 ± 16.9), and post-taper (3.7 ± 15.3). Smartphone-derived HRV, recorded upon waking, was sensitive to resistance training loads across an overload and taper microcycles in competitive strength athletes, whereas the HRV was taken immediately prior to the testing session was not.
Staying Home, Distancing, and Face Masks: COVID-19 Prevention among U.S. Women in The COPE Study
Authors: Anderson Katherine M. , Stockman Jamila K.
Published: December 2020

The novel coronavirus (COVID-19) pandemic has significantly impacted United States residents. Prevention behaviors are critical to minimizing transmission of SARS-CoV-2 in the U.S., to ultimately reduce the health, social, and economic burdens of COVID-19. Yet, health behavior decision-making is complex, and uptake of preventative behaviors has been variable. Women may provide pro-prevention behavior modeling to their networks, facilitating uptake diffusion. The COPE Study enrolled 491 women residing in the United States from May to June of 2020; women completed an online survey of COVID-19 experiences and prevention behaviors. We employed binary logistic modeling to identify factors predicting women’s practice of (1) staying home except for essential activities, (2) physical distancing in public, and (3) wearing a face mask in public. Findings demonstrate that women’s prevention behaviors are influenced by multilevel factors. Women living in urban environments, having minimal formal education, or having a household annual income of USD 30,000–50,000 are less likely to practice prevention behaviors. Cultural context may be an important factor in the decision-making process. Results aid in the identification what interventional “levers” may warrant consideration to promote uptake of such behaviors, and whom to engage. Because women are modelers of behavior, it is critical to engage them in prevention behavior interventions.
Workshops as Tools for Developing Collaborative Practice across Professional Social Worlds in Telemonitoring
Authors: Nickelsen Niels Christian Mossfeldt , Bal Roland
Published: December 2020

Background: Lately, patients suffering from chronic obstructive pulmonary disease use telemonitoring services from home. We discuss three professional groups’ idea of good care in terms of living as a chronically ill patient. Methods: We scrutinize a workshop consisting of the following: (1) presentation of pre-workshop interviews focusing on good patient flows; (2) presentation of the participants’ photos illustrating their idea of the good life with telemonitoring; (3) discussion of what the three social worlds of care can do together. We understand workshops as learning events founded on the symbolic interactionist idea of learning as reflexism. That is, the process where participants make joint action an object of attention. Results: We propose that not only people, but also objects such as applications, gold standards, and financial arrangement are actively involved in hampering collaboration across social worlds. The contribution is a discussion of the contemporary challenges of technological intensification into healthcare processes seen as a learning event. Conclusion: Workshops constitute useful tools to understand more of how professional groups seek to adopt new technologies and learn about the larger structure of telemonitoring. Developing joint action among social worlds appears to be one of the main challenges of technologically driven innovation in healthcare.
Psychosocial Barriers of Public Transport Use and Social Exclusion among Older Adults: Empirical Evidence from Lahore, Pakistan
Authors: Al-Rashid Muhammad Ahmad , Goh Hong Ching , Harumain Yong Adilah Shamsul , Ali Zulfiqar , Campisi Tiziana , Mahmood Tahir
Published: December 2020

Transport planning and public health have been intertwined historically. The health impact of public transport services, such as social exclusion, is a widely discussed research topic. Social exclusion is a paramount concern for older adults’ health in the wake of emerging global challenges. However, there remains a significant research gap on how psychosocial barriers faced by older adults in using public transport services influence the social exclusion behavior. The present research provides empirical evidence and shows the impact of certain psychosocial barriers of public transportation on older adults’ social exclusion. A total of 243 Pakistani older adults (aged 60–89 years old) voluntarily participated in this cross-sectional study. The participants provided self-reports on their psychosocial barriers (including perceived norms, attitude, personal ability, habits, neighborhood social constraints, and intention) and the corresponding social exclusion. Partial Least Square Structural Equation Modeling (PLS-SEM) was utilized for the data analysis. The structural path model supported the significant associations between psychosocial barriers and social exclusion. Except for perceived descriptive norms, all other psychosocial barriers predicted older adults’ social exclusion. The research portrays the significance of the psychosocial factors to examine social exclusion and offers practical implications for urban and transport planners. The concerned policymakers can use the research findings to develop age-sensitive, socially sustainable, and healthy cities.
Postnatal Care Service Utilisation in Ethiopia: Reflecting on 20 Years of Demographic and Health Survey Data
Authors: Mekonnen Tensae , Dune Tinashe , Perz Janette , Ogbo Felix Akpojene
Published: December 2020

Background: Most maternal deaths in the world occur during the postpartum period, especially within the first two days following delivery. This makes postnatal care (PNC) critical to improving the chances of maternal and child survival. Over the past 20 years, the proportion of women receiving antenatal care (ANC) in Ethiopia has increased while the proportion of those receiving PNC has remained low. This study aimed to understand the trends, determinants and urban–rural variations of PNC service utilisation. Methods: This study draws on the Ethiopian Demographic and Health Survey (EDHS) data for the years 2000 (n = 4552), 2005 (n = 4467), 2011 (n = 4445) and 2016 (n = 4275) to estimate the trends and determinants of PNC service utilisation. Multivariate logistic regression models with adjustment for clustering and sampling weights were used to investigate the association between the independent factors, the study factors and PNC service utilisation. Results: Over the twenty-year period of the EDHS, the proportion of Ethiopian women who received PNC services increased from 5.6% (95% CI: 4.6–6.9%) in 2000 to 18.5% (95% CI: 16.4–20.7%) in 2016. Similarly, women who received PNC services in urban areas increased from 15.2% (95% CI: 23.6–30.7%) in 2000 to 47% (95% CI: 60.4–67.3%) in 2016. Women who were in the wealthy quintile, had ANC visits, delivered in a health facility, and delivered by caesarean section were most likely to have PNC. The present study also showed that whilst birth spacing was a significant factor among urban women, wealth index, ANC visits, and perception of health facility distance were significant factors among rural women. Conclusions: The study suggests low levels of utilisation of PNC among Ethiopian women from rural districts. Geographically targeted interventions with a focus on low-socioeconomic rural women, and those with no previous contacts with the health system during pregnancy, are needed to improve PNC in Ethiopia.
Calmodulin Supports TRPA1 Channel Association with Opioid Receptors and Glutamate NMDA Receptors in the Nervous Tissue
Authors: Cortés-Montero Elsa , Rodríguez-Muñoz María , Ruiz-Cantero M. Carmen , Cobos Enrique J. , Sánchez-Blázquez Pilar , Garzón-Niño Javier
Published: December 2020

Transient receptor potential ankyrin member 1 (TRPA1) belongs to the family of thermo TRP cation channels that detect harmful temperatures, acids and numerous chemical pollutants. TRPA1 is expressed in nervous tissue, where it participates in the genesis of nociceptive signals in response to noxious stimuli and mediates mechanical hyperalgesia and allodynia associated with different neuropathies. The glutamate N-methyl-d-aspartate receptor (NMDAR), which plays a relevant role in allodynia to mechanical stimuli, is connected via histidine triad nucleotide-binding protein 1 (HINT1) and type 1 sigma receptor (σ1R) to mu-opioid receptors (MORs), which mediate the most potent pain relief. Notably, neuropathic pain causes a reduction in MOR antinociceptive efficacy, which can be reversed by blocking spinal NMDARs and TRPA1 channels. Thus, we studied whether TRPA1 channels form complexes with MORs and NMDARs that may be implicated in the aforementioned nociceptive signals. Our data suggest that TRPA1 channels functionally associate with MORs, delta opioid receptors and NMDARs in the dorsal root ganglia, the spinal cord and brain areas. These associations were altered in response to pharmacological interventions and the induction of inflammatory and also neuropathic pain. The MOR-TRPA1 and NMDAR-TRPA1 associations do not require HINT1 or σ1R but appear to be mediated by calcium-activated calmodulin. Thus, TRPA1 channels may associate with NMDARs to promote ascending acute and chronic pain signals and to control MOR antinociception.
Current Trends and Future Prospects of Molecular Targeted Therapy in Head and Neck Squamous Cell Carcinoma
Authors: Kitamura Naoya , Sento Shinya , Yoshizawa Yasumasa , Sasabe Eri , Kudo Yasusei , Yamamoto Tetsuya
Published: December 2020

In recent years, advances in drug therapy for head and neck squamous cell carcinoma (HNSCC) have progressed rapidly. In addition to cytotoxic anti-cancer agents such as platinum-based drug (cisplatin and carboplatin) and taxane-based drugs (docetaxel and paclitaxel), epidermal growth factor receptor-tyrosine kinase inhibitors (cetuximab) and immune checkpoint inhibitors such as anti-programmed cell death-1 (PD-1) antibodies (nivolumab and pembrolizumab) have come to be used. The importance of anti-cancer drug therapy is increasing year by year. Therefore, we summarize clinical trials of molecular targeted therapy and biomarkers in HNSCC from previous studies. Here we show the current trends and future prospects of molecular targeted therapy in HNSCC.
Androgen Reduces Mitochondrial Respiration in Mouse Brown Adipocytes: A Model for Disordered Energy Balance in Polycystic Ovary Syndrome
Authors: Lerner Avi , Kewada Drashti , Ahmed Ayan , Hardy Kate , Christian Mark , Franks Stephen
Published: December 2020

Polycystic ovary syndrome (PCOS) is a common endocrinopathy that is associated with an adverse metabolic profile including reduced postprandial thermogenesis. Although abnormalities in adipose tissue function have been widely reported in women with PCOS, less is known about direct effects of androgen on white and, particularly, brown adipocytes. The purpose of this study was to investigate the effect of the nonaromatizable androgen dihydrotestosterone (DHT) on (1) lipid accumulation and expression of adipogenic markers in immortalized mouse brown adipose cell lines (IMBATs), (2) mitochondrial respiration in IMBATs, (3) mitochondrial DNA content and gene expression, (4) expression of brown adipose tissue (BAT) markers and thermogenic activation. In addition, we profiled the relative levels of 38 adipokines secreted from BAT explants and looked at androgen effects on adipokine gene expression in both IMBATs and immortalized mouse white adipose (IMWATs) cell lines. Androgen treatment inhibited IMBAT differentiation in a dose-dependent manner, reduced markers of adipogenesis, and attenuated the β-adrenoceptor-stimulated increase in uncoupling protein-1 (UCP1) expression. In explants of mouse interscapular BAT, androgen reduced expression of UCP1, peroxisome proliferator-activated receptor-γ coactivator-1 (PCG-1) and Cidea. Significantly, as well as affecting genes involved in thermogenesis in BAT, androgen treatment reduced mitochondrial respiration in IMBATs, as measured by the Seahorse XF method. The results of this study suggest a role for excess androgen in inhibiting brown adipogenesis, attenuating the activation of thermogenesis and reducing mitochondrial respiration in BAT. Together, these data provide a plausible molecular mechanism that may contribute to reduced postprandial thermogenesis and the tendency to obesity in women with PCOS.
Correction: Karimi, P., et al. Crocetin Prevents RPE Cells from Oxidative Stress through Protection of Cellular Metabolic Function and Activation of ERK1/2. Int. J. Mol. Sci. 2020, 21, 2949
Authors: Karimi Padideh , Gheisari Ali , Gasparini Sylvia J. , Baharvand Hossein , Shekari Faezeh , Satarian Leila , Ader Marius
Published: December 2020

The authors wish to make the following correction to Figure 6B of this article [...]
Infrared Comb Spectroscopy of Buffer-Gas-Cooled Molecules: Toward Absolute Frequency Metrology of Cold Acetylene
Authors: Santamaria Luigi , Di Sarno Valentina , Aiello Roberto , De Rosa Maurizio , Ricciardi Iolanda , De Natale Paolo , Maddaloni Pasquale
Published: December 2020

We review the recent developments in precision ro-vibrational spectroscopy of buffer-gas-cooled neutral molecules, obtained using infrared frequency combs either as direct probe sources or as ultra-accurate optical rulers. In particular, we show how coherent broadband spectroscopy of complex molecules especially benefits from drastic simplification of the spectra brought about by cooling of internal temperatures. Moreover, cooling the translational motion allows longer light-molecule interaction times and hence reduced transit-time broadening effects, crucial for high-precision spectroscopy on simple molecules. In this respect, we report on the progress of absolute frequency metrology experiments with buffer-gas-cooled molecules, focusing on the advanced technologies that led to record measurements with acetylene. Finally, we briefly discuss the prospects for further improving the ultimate accuracy of the spectroscopic frequency measurement.
The Lipid A from the Lipopolysaccharide of the Phototrophic Bacterium Rhodomicrobium vannielii ATCC 17100 Revisited
Authors: Komaniecka Iwona , Susniak Katarzyna , Choma Adam , Heine Holger , Holst Otto
Published: December 2020

The structure of lipid A from lipopolysaccharide (LPS) of Rhodomicrobium vannielii ATCC 17100 (Rv) a phototrophic, budding bacterium was re-investigated using high-resolution mass spectrometry, NMR, and chemical degradation protocols. It was found that the (GlcpN)-disaccharide lipid A backbone was substituted by a GalpA residue that was connected to C-1 of proximal GlcpN. Some of this GalpA residue was β-eliminated by alkaline de-acylation, which indicated the possibility of the presence of another so far unidentified substituent at C-4 in non-stoichiometric amounts. One Manp residue substituted C-4′ of distal GlcpN. The lipid A backbone was acylated by 16:0(3-OH) at C-2 of proximal GlcpN, and by 16:0(3-OH), i17:0(3-OH), or 18:0(3-OH) at C-2′ of distal GlcpN. Two acyloxy-acyl moieties that were mainly formed by 14:0(3-O-14:0) and 16:0(3-O-22:1) occupied the distal GlcpN of lipid A. Genes that were possibly involved in the modification of Rv lipid A were compared with bacterial genes of known function. The biological activity was tested at the model of human mononuclear cells (MNC), showing that Rv lipid A alone does not significantly stimulate MNC. At low concentrations of toxic Escherichia coli O111:B4 LPS, pre-incubation with Rv lipid A resulted in a substantial reduction of activity, but, when higher concentrations of E. coli LPS were used, the stimulatory effect was increased.
Assessment of Synthetic Membranes for Artificial Blood Feeding of Culicidae
Authors: Dias Luciana S. , Caldeira Jonatas C. , Bauzer Luiz G. S. R. , Lima José B. P.
Published: December 2020

Potential pathogen transmission through hematophagy in Culicidae is a major public-health problem, and several studies have been performed to better understand this phenomenon. Research on these insects often requires the maintenance of colonies in the laboratory. Due to the hematophagic habits of these organisms, blood must be provided in order to guarantee the reproduction of individuals that constitute the colonies. Some species of mammals and birds are used as a direct blood source in many laboratories. Due to current bioethical parameters, the direct use of animals has been replaced by artificial blood feeding by using synthetic membranes to simulate animal skin. In this study, the efficiency of collagen and latex in the artificial feeding of mosquitoes of the Aedes aegypti and Culex quinquefasciatus species was evaluated and compared with Parafilm®, a standard membrane that is frequently used for this purpose. Important aspects of the feeding and reproduction of these insects were considered. For both species, latex showed the poorest performance. Collagen membrane performed well in most parameters, but was not as efficient as Parafilm® for fecundity in Aedes aegypti, and for the percentage of engorged females in Culex quinquefasciatus. We concluded that, although collagen is more resistant and easier to handle, Parafilm® was the most efficient among the three evaluated membranes for the artificial blood feeding of mosquitoes.
WRKY Transcription Factors in Nicotiana tabacum Modulate Plant Immunity against Whitefly via Interacting with MAPK Cascade Pathways
Authors: Yao Dan-Mei , Zou Chi , Shu Yan-Ni , Liu Shu-Sheng
Published: December 2020

WRKY transcription factors are key regulators of many plant processes, most notably coping with biotic and abiotic stresses. Recently, the function of WRKY in plant defense against phloem-feeding insects such as whitefly (Bemisia tabaci) has been brought to attention. In this study, we found that the expression levels of Nicotiana tabacum WRKY4, WRKY6 and WRKY10 were significantly upregulated when tobacco plants were infested with whiteflies or treated with salicylic acid. Compared to controls, whiteflies lived longer and laid more eggs on NtWRKY-silenced tobacco plants but performed less well on NtWRKY-overexpressing plants. The three NtWRKYs interacted with five mitogen-activated protein kinases (NtMAPKs) in vivo and in vitro. These results suggest that the WRKYs in tobacco positively modulate plant defense against whiteflies through interaction with the mitogen-activated protein kinase cascade (MAPK cascade) pathways, and thus provide new insights into plant defense against phloem-feeding insects.
Energy Efficiency for Social Buildings in Morocco, Comparative (2E) Study: Active VS. Passive Solutions Via TRNsys
Authors: Gargab Fatima Zohra , Allouhi Amine , Kousksou Tarik , El-Houari Haytham , Jamil Abdelmajid , Benbassou Ali
Published: December 2020

This paper aims to highlight the potential of solar water heater installations in Morocco. The project involves the comparison of active and passive solutions for energy efficiency in buildings. To this end, a numerical simulation model of solar water heater installations is created under TRNsys. Three hot water demand scenarios (Low, Standard, and High) were taken into account for the six climatic zones defined in the Moroccan thermal regulation of constructions. The same software (TRNsys) is used to model a pilot building consisting of 16 flats. Energy efficiency actions have been applied to the building envelope (insulation and glazing) and simulations are made for the six areas. The simulation results comparing energy and financial savings show the influence of subsidized gas prices on solar water heaters’ relevance despite significant energy savings. This work proves that solar water heaters will be a primary obligation for Morocco, taking into account changes in butane gas prices.
MixInYeast: A Multicenter Study on Mixed Yeast Infections
Authors: Medina Narda , Soto-Debrán Juan , Seidel Danila , Akyar Isin , Badali Hamid , Barac Aleksandra , Bretagne Stéphane , Cag Yasemin , Cassagne Carole , Castro Carmen , Chakrabarti Arunaloke , Dannaoui Eric , Cardozo Celia , Garcia-Rodriguez Julio , Guitard Juliette , Hamal Petr , Hoenigl Martin , Jagielski Tomasz , Khodavaisy Sadegh , Lo Cascio Giuliana , Martínez-Rubio María , Meletiadis Joseph , Muñoz Patricia , Ochman Elżbieta , Peláez Teresa , Perez-Ayala Balzola Ana , Prattes Juergen , Roilides Emmanuel , Ruíz-Pérez de Pipaón Maite , Stauf Raphael , Steinmann Jörg , Suárez-Barrenechea Ana , Tejero Rocío , Trovato Laura , Viñuela Lourdes , Wongsuk Thanwa , Żak Iwona , Zarrinfar Hossein , Lass-Flörl Cornelia , Arikan-Akdagli Sevtap , Alastruey-Izquierdo Ana ,   on behalf of MixInYeast Study Group from EFISG
Published: December 2020

Invasive candidiasis remains one of the most prevalent systemic mycoses, and several studies have documented the presence of mixed yeast (MY) infections. Here, we describe the epidemiology, clinical, and microbiological characteristics of MY infections causing invasive candidiasis in a multicenter prospective study. Thirty-four centers from 14 countries participated. Samples were collected in each center between April to September 2018, and they were sent to a reference center to confirm identification by sequencing methods and to perform antifungal susceptibility testing, according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST). A total of 6895 yeast cultures were identified and MY occurred in 150 cases (2.2%). Europe accounted for the highest number of centers, with an overall MY rate of 4.2% (118 out of 2840 yeast cultures). Of 122 MY cases, the most frequent combinations were Candida albicans/C. glabrata (42, 34.4%), C. albicans/C. parapsilosis (17, 14%), and C. glabrata/C. tropicalis (8, 6.5%). All Candida isolates were susceptible to amphotericin B, 6.4% were fluconazole-resistant, and two isolates (1.6%) were echinocandin-resistant. Accurate identification of the species involved in MY infections is essential to guide treatment decisions.
Semi-Destructive and Non-Destructive Tests of Timber Structure of Various Moisture Contents
Authors: Jaskowska-Lemańska Justyna , Przesmycka Elżbieta
Published: December 2020

The condition of heritage and historic timber constructions depends on how they are exploited. Numerous environmental factors degrade the physical and mechanical properties of timber and hence, affect the load-bearing capacity of constructions. As a result, frequent evaluations of their technical condition become necessary. Currently, modern technologies allow for extensive diagnostics of timber constructions using non-destructive and semi-destructive methods; yet, in contrast to classical laboratory tests, there is insufficient knowledge of the impact of individual factors on the results of such studies. This article presents an assessment of the influence of the moisture content of timber elements on the results of ultrasonic stress wave, sclerometric, and resistance drilling tests. Additionally, computed tomography scans were performed on selected samples to demonstrate the destruction mechanism occurring during the semi-destructive tests. The research involved three types of wood: pine, spruce, and fir of different moisture contents. The results reveal a strong relation between the moisture of timber and all the tests conducted in terms of both hygroscopic and capillary moisture.
Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance System
Authors: Matsumoto Takumi , Tashiro Yuichiro , Komasa Satoshi , Miyake Akiko , Komasa Yutaka , Okazaki Joji
Published: December 2020

Primary stability and osseointegration are major challenges in dental implant treatments, where the material surface properties and wettability are critical in the early formation of hard tissue around the implant. In this study, a quartz crystal microbalance (QCM) was used to measure the nanogram level amount of protein and bone marrow cells adhered to the surfaces of titanium (Ti) surface in real time. The effects of ultraviolet (UV) and atmospheric-pressure plasma treatment to impart surface hydrophilicity to the implant surface were evaluated. The surface treatment methods resulted in a marked decrease in the surface carbon (C) content and increase in the oxygen (O) content, along with super hydrophilicity. The results of QCM measurements showed that adhesion of both adhesive proteins and bone marrow cells was enhanced after surface treatment. Although both methods produced implants with good osseointegration behavior and less reactive oxidative species, the samples treated with atmospheric pressure plasma showed the best overall performance and are recommended for clinical use. It was verified that QCM is an effective method for analyzing the initial adhesion process on dental implants.