bluuebunny/crossref_metadata_2025 huggingface dataset:
Diatom and Dinocyst Production, Composition and Flux from the Annual Cycle Sediment Trap Study in the Barents Sea
Authors: Agafonova Elizaveta , Novichkova Ekaterina , Novigatsky Alexander , Kravchishina Marina , Klyuvitkin Alexey , Bulokhov Anton
Published: December 2022

This paper presents the diatom and palynomorph data from a sediment trap deployed in the northern part of the East Barents Sea for an annual cycle from August 2017 to August 2018. The average monthly fluxes of diatoms and dinoflagellate cysts in the photic layer of the northeastern part of the Barents Sea varies from 10.4 × 103 to 640.8 × 103 valves m−2 day−1 and from 0.3 × 103 to 90.0 × 103 cysts m−2 day−1, respectively. Their fluxes are related to the low irradiance of the photic layer during the sea-ice cover period, dominance of southward currents, modern climate, and nepheloid layer conditions. Based on redundancy analysis of the relationship between the fluxes of diatoms and dinoflagellate cysts and organic carbon fluxes, sea-ice covers, and the seasonal cycle of light availability we determined the following. First, sea-ice-associated diatoms and dinocysts are exported to the sediment trap from the melting sea ice with a two-week delay. Second, the appearance of freshwater diatoms and green algae in the sinking material accumulating from March 2018 to July 2018 is also related to the melting of sea ice. And third, the presence of Coscinodiscus radiatus, C. perforatus, Shionodiscus oestrupii and Operculodinium centrocarpum in the diatoms and dinocysts species composition throughout the year indicates the advection of Atlantic waters into the Barents Sea up to 80° N.
Using a Motivational Paradigm to Develop an Exercise Program for Nurses with High Risk of Metabolic Syndrome
Authors: Lee Wen-Ping , Wu Pao-Yuan , Chen Li-Chin , Shih Whei-Mei
Published: December 2022

Nurses are frontline care providers whose health is vital to providing good quality of care to patients. The purpose of this study was to develop an exercise program for high-risk metabolic syndrome nurses based on the transtheoretical model. The transtheoretical model was used in this study due to its popular use in exercise behavior change and it can clearly identify the stage of exercise so as to plan an effective program to promote health. This was a quasi-experimental pilot study with a total of 40 participants who met the inclusion criteria. Exercise programs were developed for three groups distinguished by their commitment to exercising for health. Sixteen (40%) nurses moved one step forward, six (15%) nurses moved backward, and eighteen (45%) nurses maintained at the same stage over time (stable sedentary, 40%; stable active, 5%). Bowker’s test of symmetry, χ2 = 14.00 (p < 0.01), revealed that the population exercising increased significantly after the intervention. After the program, the perceived benefits from exercise in the decisional balance significantly increased to 1.53 (t = 2.223, p < 0.05), perceived exercise barriers significantly decreased to 3.10 (t = −3.075, p < 0.05), and self-efficacy significantly increased to 2.90 (t = 3.251, p < 0.01), respectively. Applying the transtheoretical model to health behavior enables significant change. The benefits of applying the transtheoretical model for promoting exercise include increasing perceived exercise benefits and self-efficacy, decreasing perceived exercise barriers, and increasing physical activity levels.
Diagnostic Performances of Ultrasound-Based Models for Predicting Malignancy in Patients with Adnexal Masses
Authors: Velayo Clarissa L. , Reforma Kareen N. , Sicam Renee Vina G. , Diwa Michele H. , Sy Alvin Duke R. , Tantengco Ourlad Alzeus G.
Published: December 2022

This study compared the diagnostic performance of different ultrasound-based models in discriminating between benign and malignant ovarian masses in a Filipino population. This was a prospective cohort study in women with findings of an ovarian mass on ultrasound. All included patients underwent a physical examination before level III specialist ultrasonographic and Doppler evaluation using the different International Ovarian Tumor Analysis (IOTA) Group’s risk models. Serum CA-125 and a second-generation multivariate index assay (MIA2G) were also determined for all patients. The ovarian imaging and biomarker results were correlated with the histological findings. A total of 260 patients with completed ultrasound, CA-125, MIA2G, and histopathologic results was included in the study. The presence of papillae with blood flow and irregular cyst walls during the ultrasound were significantly associated with a 20-fold (OR: 20.13, CI: 8.69–46.67, p < 0.01) and 10-fold (OR: 10.11, CI: 5.30–19.28, p < 0.01) increase in the likelihood of a malignant lesion, respectively. All individual sonologic procedures performed well in discerning malignant and benign ovarian lesions. IOTA-LR1 showed the highest accuracy (82.6%, 95% CI: 77.5–87%) for identifying ovarian cancer. IOTA-ADNEX showed the highest sensitivity (93.3%, 95% CI: 87.2–97.1%) while IOTA-LR2 exhibited the highest specificity (84.4%, 95% CI: 77.3–90%). Among the different serial test combinations, IOTA-LR1 with MIA2G and IOTA-LR2 with MIA2G showed the highest diagnostic accuracy (AUROC = 0.82). This study showed that all individual ultrasound-based models performed well in discerning malignant and benign ovarian lesions, with IOTA-LR1 exhibiting the highest accuracy.
Development and Assessment of the Scale of Personal Trust and Connections (PerTC): Preliminary Data from a Hospital Employee Group
Authors: Symvoulakis Emmanouil K. , Volkos Panagiotis , Kamekis Apostolos , Merou Konstantina , Rachiotis Georgios , Morgan Myfanwy , Linardakis Manolis
Published: December 2022

Trust and empathy constitute basic elements of healthcare delivery. In recent years, the quest for greater efficiency in healthcare has also indicated the necessity of these values. The study aims to develop and assess a 10-item tool, namely, the Personal Trust and Connections (PerTC) scale. The study was conducted at a general hospital in eastern Crete, Greece. A total of 218 healthcare professionals participated over a six-week period in 2021. The 10-item PerTC scale encompasses emotional, social, and cognitive reliance variables. The scale was tested for reliability, and scale scores were assessed for convergent validity. PerTC scale was found with high internal consistency (Cronbach’s α = 0.863). At a multivariate level, younger age (p = 0.016), more work experience years (p = 0.001), the experience of a recent family crisis event (p = 0.028), and use of the internet in free time (p = 0.028) were significantly related to increased total scores of the PerTC scale. The new scale is an easy-to-use metric tool with good overall reliability. PerTC may be a suitable instrument to indirectly identify determinants and drivers in order to explore pathways to collectively build on trustful interaction and altruistic connection within a healthcare environment.
Definite Identifiers of Silver Dye Bleach Prints
Authors: Chun Suk Fong , Hofmann-Sievert Rita , Stigter Sanneke
Published: December 2022

Among diverse contemporary colour prints, silver dye bleach prints and chromogenic prints are difficult to differentiate. They share similar visual characteristics and can use identical supports and surface finishes. However, their image-forming dyes differ, resulting in disparate conservation and restoration needs. This study aimed to determine practical measures for unambiguously differentiating between these two print types. Identifying characteristics—referred to here as ‘identifiers’—were collected from popular conservation sources and a mixed-method questionnaire survey. The accuracy and feasibility of these identifiers were evaluated against known prints sets. Examinations made use of water droplets, various light sources, digital 3D microscopy, and spectrophotometry. Results dichotomised these identifiers into ‘definite’ or ‘indefinite’ with ‘definite identifiers’ being able to discriminate independently. Only five out of 23 entries were termed definite identifiers. Azo dyes—image dyes of silver dye bleach prints—were established as the only constant definite identifiers of this print type. These findings were integrated into a flowchart to guide differentiation with the main recommendation being to deviate from indefinite identifiers to save time and effort. Parts of this work have been submitted in partial fulfilment of the requirements for a Master of Science degree at the University of Amsterdam in 2020.
Dye Identification in Mounting Textiles of Traditional Korean Paintings from the Late Joseon Dynasty
Authors: Tamburini Diego , Kim-Marandet Meejung , Kim Sang-ah
Published: December 2022

In the framework of the ‘Amorepacific Project for the conservation of Korean pictorial art’ (2018–2023) at the British Museum, three traditional Korean paintings have been investigated with the aim of supporting their conservation and obtaining information about the dyes used in the mounting textiles and other mounting elements. The paintings include a rare example of late 18th-century traditional Korean portraiture (accession number 1996,0329,0.1); a late 19th-century two-panel screen silk painting of Pyeongsaeng-do-Scenes of life (accession number 2016,3028.1); and a late 19th-century twelve-panel screen silk painting representing the Five Confucian virtues (accession number 1957,1214,0.1). The mounting textiles were investigated non-invasively by using digital microscopy and fibre optic reflectance spectroscopy (FORS), and the results guided a minimally invasive sampling campaign. Fourteen samples were analysed by using high-pressure liquid chromatography coupled with diode array and tandem mass spectrometry detectors (HPLC-DAD-MS/MS), leading to the identification of the natural dyes indigo, sappanwood (Biancaea sappan, formerly Caesalpinia sappan), amur cork tree (Phellodendron amurense) and safflower (Carthamus tinctorius) in the mounting elements of the 18th-century portrait. These results confirmed some of the non-invasive observations and were in agreement with the production date of the painting. Both natural and synthetic dyes were identified in the mounting textiles of the panel screens. Among the synthetic dyes, fuchsin (C.I. 42510), methyl violet 3B (C.I. 42536), methyl blue (C.I. 42780) and benzopurpurin 4B (C.I. 23500) were identified. These are early synthetic dyes first synthesised between the 1860s and the 1880s, suggesting that the silk textiles are likely to have been dyed in the last part of the 19th century.
Janissaries and Urban Notables in Local Politics: Struggle for Power and Factional Strife in the Late Eighteenth-Century Anatolian Town of Adana
Authors: Yıldız Aysel
Published: December 2022

The transformations that occurred in the Ottoman Empire in the eighteenth century, summarized by one author as more army, more taxes, more bureaucracy, and more state intrusion in the Ottoman provinces, radically changed provincial life in the Ottoman domains. Growing tax and manpower demands not only increased socio-economic pressure on the provinces but also redefined the sultan’s relationship with local authorities. Accompanied by the increasingly frequent stationing of the Janissary corps in the Ottoman provinces, especially in the seventeenth century, the Ottoman cities and towns saw new elite configurations and new types of power struggles and came under greater economic pressure. The rising number of registered Janissaries changed the internal dynamics of the towns, shaped local politics, and created new struggles for power in the cities where corps regiments were stationed, pushing the Janissaries into local politics, whether as rivals or allies of the local elite. As elsewhere, the southern Anatolian town of Adana witnessed such changes in its social structure, local politics, and relations with the imperial authority. Although similarities are to be seen with the eighteenth century provincial power struggles in the Anatolian and Arabian cities of Gaziantep and Aleppo in terms of intense factional strife and the active involvement of the Janissaries and their pretenders in local politics, the power struggle in Adana was between several Janissary officers, one of whom subsequently managed to become the urban notable (ayan) of the town.
A Novel Model Fusion Approach for Greenhouse Crop Yield Prediction
Authors: Gong Liyun , Yu Miao , Cutsuridis Vassilis , Kollias Stefanos , Pearson Simon
Published: December 2022

In this work, we have proposed a novel methodology for greenhouse tomato yield prediction, which is based on a hybrid of an explanatory biophysical model—the Tomgro model, and a machine learning model called CNN-RNN. The Tomgro and CNN-RNN models are calibrated/trained for predicting tomato yields while different fusion approaches (linear, Bayesian, neural network, random forest and gradient boosting) are exploited for fusing the prediction result of individual models for obtaining the final prediction results. The experimental results have shown that the model fusion approach achieves more accurate prediction results than the explanatory biophysical model or the machine learning model. Moreover, out of different model fusion approaches, the neural network one produced the most accurate tomato prediction results, with means and standard deviations of root mean square error (RMSE), r2-coefficient, Nash-Sutcliffe efficiency (NSE) and percent bias (PBIAS) being 17.69 ± 3.47 g/m2, 0.9995 ± 0.0002, 0.9989 ± 0.0004 and 0.1791 ± 0.6837, respectively.
Growth and Yield of Okra Exposed to a Consortium of Rhizobacteria with Different Organic Carriers under Controlled and Natural Field Conditions
Authors: Perveen Rabia , Hussain Azhar , Ditta Allah , Dar Abubakar , Aimen Ayesha , Ahmad Maqshoof , Alataway Abed , Dewidar Ahmed Z. , Mattar Mohamed A.
Published: December 2022

Crop yields, soil fertility, and soil quality decline due to the overuse of chemical fertilizers and other agrochemicals. The damaging effects of these agrochemicals on the environment can be minimized by integration with eco-friendly approaches, i.e., biofertilizers. These eco-friendly biofertilizers containing plant growth-promoting rhizobacteria, (PGPR) not only solubilize mineral nutrients for crop uptake but also release phytohormones for their growth improvements. The objective of this research is to use these PGPR’s capacity to promote growth in order to increase okra production. For this purpose, different organic carriers were used, i.e., Press mud, Charcoal, Biochar, Peat, and Compost for PGPR’s inoculation. Before being used as a consortium with various carrier materials, the pre-isolated and characterized PGPR strains (AN-35, ZM-27, and ZM-63) were tested for compatibility against one another. The PGPR consortium and carriers were applied in the following treatments, i.e., T0: (control), T1: PGPR, T2: Peat + PGPR, T3: Pressmud+ PGPR, T4: Compost + PGPR, T5: Charcoal + PGPR, and T6: Biochar + PGPR in the present pot and field studies. Under the pot experiment, the results depicted that all treatments showed a significant increase in okra growth, nutrient contents, and yield of okra along with increasing the microbial biomass in the soil but the treatment containing PGPR consortium with peat caused the maximum increase. Similarly, the results of the field experiment also showed a significant increase under all treatments but the maximum increase in nutrient contents, growth attributes, and yield of okra was found under the treatment containing PGPR consortium with peat (T2). Therefore, this study recommends the use of peat and studied the PGPR consortium as a suitable carrier to develop carrier-based biofertilizers for sustainable okra production.
Low Activation of Knee Extensors and High Activation of Knee Flexors in Female Fencing Athletes Is Related to the Response Time during the Marche-Fente
Authors: Kim Tae-Whan , Lee Jin-seok , Jo Iseul
Published: December 2022

Reaction time is important to determine the performance of fencing. The purpose of this study was to investigate the reaction time and muscle activity and compare the movement among genders during Marche-fente. Fifteen Korean national Fleuret fencing athletes participated and were instructed to perform Marche-fente. Reaction time was measured with Plug & Play equipment and muscle activation was obtained by electromyography. The male athletes (0.94 ± 0.08 s) were faster than female athletes, who a performance of 1.03 ± 0.05 s. As the knee extensors activation was increased, the movement and response time was shorter (rectus femoris (RF); r = −0.526, p < 0.05, vastus lateralis oblique (VLO); r = −0.628, p < 0.05). In phase 1, men activated more knee extensors in the dominant leg, whereas the activation of knee flexors was increased to maintain a stable posture in women. Additionally, women used other muscles instead of large muscles such as RF and VLO in phase 2. In conclusion, female athletes activated knee flexors rather than knee extensors when moving the center of mass or generating a greater force. Less use of knee extensors is associated with knee injuries; therefore, exercise which activates knee extensors is required for females.
Convergent Validation of a Self-Reported Commuting to and from School Diary in Spanish Adolescents
Authors: Gálvez-Fernández Patricia , Herrador-Colmenero Manuel , Campos-Garzón Pablo , Molina-Soberanes Daniel , Saucedo-Araujo Romina Gisele , Aranda-Balboa María Jesús , Lara-Sánchez Amador Jesús , Segura-Jiménez Víctor , Henriksson Pontus , Chillón Palma
Published: December 2022

The aim of this study was to examine the convergent validity of self-reported diary times for commuting to and from school with device-measured positional data (Global Positioning System; GPS) in Spanish adolescents. Methods: Cross-sectional data were obtained from four Spanish public secondary schools in 2021, comprising 47 adolescents and 141 home–school and school–home trips. Participants self-reported the time they left and arrived at home and school through a commuting diary. They wore a GPS device recording the objective time during three trips (i.e., one home–school trip and two school–home trips). Agreement between commuting diary and GPS data regarding home–school trips and school–home trips was evaluated using Bland–Altman plots. Results: Total commuting time differed by 1 min (95% limits of agreement were 16.1 min and −18.1 min) between subjective and objective measures (adolescents reported 0.8 more minutes in home–school trips and 1 more minute in school–home trips compared to objective data). Passive commuters reported 0.7 more minutes and active commuters reported 1.2 more minutes in the total commuting time compared to objective data. Conclusions: Self-reported commuting diaries may be a useful tool to obtain commuting times of adolescents in epidemiological research or when tools to measure objective times are not feasible.
The Effect of Mealworm Frass on the Chemical and Microbiological Properties of Horticultural Peat in an Incubation Experiment
Authors: Nogalska Anna , Przemieniecki Sebastian Wojciech , Krzebietke Sławomir Józef , Załuski Dariusz , Kosewska Agnieszka , Skwierawska Małgorzata , Sienkiewicz Stanisław
Published: December 2022

Insect farming is growing in popularity, and in addition to insect meal, it generates waste products such as exuviae and frass, which can be recycled in agriculture. The aim of this incubation experiment was to evaluate the effect of Tenebrio molitor L. frass on selected chemical and biological properties of deacidified peat, which is widely used in horticulture. The optimal rate of frass fertilizer in peat for growing vegetables and ornamental plants was determined, with special emphasis on mineral nitrogen levels. Peat was fertilized with five nitrogen rates, 0, 50, 100, 200, and 400 mg dm−3, and supplied with frass or urea. The study demonstrated that frass can be used as organic fertilizer. An increase in the nitrogen rate significantly increased mineral nitrogen content and electrical conductivity and decreased Ca content in peat. Both frass and urea increased the ammonification rate at the beginning of incubation and the nitrification rate from the second week of the experiment. Higher frass rates (5 and 10 g dm−3) increased the content of plant-available nutrients (nitrogen, phosphorus, magnesium, potassium, and sodium) in peat as well as the abundance of microorganisms supporting organic matter mineralization. Unlike frass, urea increased the counts of nitrogen-fixing bacteria in peat.
“It Needs a Full-Time Dedicated Person to Do This Job in Our Local Communities with Our Aboriginal Health Services”—Aboriginal and Torres Strait Islander Health Workers and Practitioners Perspectives on Supporting Smoking Cessation during Pregnancy
Authors: Mersha Amanual Getnet , Maddox Raglan , Maidment Sian , Booth Kade , Briscoe Karl , Hussein Paul , Longbottom Hayley , Bar-Zeev Yael , Kennedy Michelle
Published: December 2022

Background: Aboriginal and Torres Strait Islander women deserve improved smoking cessation support. Aboriginal health workers (AHW) and practitioners (AHP) can be central to the provision of culturally safe smoking cessation care (SCC). The objective of this study is to explore attitudes and the perceived role of AHWs/AHPs toward providing SCC to Aboriginal and Torres Strait Islander pregnant women. Method: A mixed-method study using quantitative and qualitative data was conducted among AHW/AHPs in 2021 across Australia. Descriptive and analytical statistics were used to characterise AHWs’/AHPs’ attitudes towards SCC and to evaluate the factors associated with perceptions of who is best placed to provide SCC. Results: From the total AHW/AHP workforce, 21.2% (223) completed the survey. Less than half (48.4%) believed that AHW/AHP were best placed to provide SCC for pregnant women. The majority believed that group-based supports (82.5%) and cultural support programs (63.7%) were the best strategies to support Aboriginal and Torres Strait Islander pregnant women to quit smoking. Conclusion: This study highlights the need to enhance SCC offered to Aboriginal and Torres Strait Islander pregnant women. A targeted workforce dedicated to smoking cessation should be resourced, including funding, standardised training, and ongoing SCC support tailored to Aboriginal and Torres Strait Islander pregnant women.
Can Population Mobility Make Cities More Resilient? Evidence from the Analysis of Baidu Migration Big Data in China
Authors: Chen Yu , Li Keyang , Zhou Qian , Zhang Yuxin
Published: December 2022

Knowledge spillover and capital agglomeration caused by population migration behavior are of great significance for improving the carrying capacity and adaptability of the urban economy and promoting high-quality economic development. Based on the big data collected on urban migration during the Spring Festival travel period, this paper constructs geographic, economic and geo-economic matrices, introduces two instrumental variables, and uses a spatial econometric model to investigate the mechanism between population mobility and urban economic resilience. The results show that (1) urban economic resilience exhibits spatial correlation, and the correlation order is geo-economic matrix > economic matrix > geography matrix; (2) the economic resilience of inflow areas is significantly affected by the net inflow of population, and the urban economic resilience index increases by 0.36–0.56% when the population mobility index increases by one unit; (3) in the case of economic and geo-economic matrices, there is a spatial interaction relationship of neighbor-companion in the mechanism of population migration on urban economic resilience; and (4) the mechanism is significantly impacted by innovation input and fixed asset investment, with positive moderating effects. In the geographical and economic matrices, the innovation input effect has a negative externality, while in the economic and geo-economic matrices, the fixed asset investment effect has a positive externality.
The Influence of Body Mass Index on Growth Factor Composition in the Platelet-Rich Plasma in Patients with Knee Osteoarthritis
Authors: Wiciński Michał , Szwedowski Dawid , Wróbel Łukasz , Jeka Sławomir , Zabrzyński Jan
Published: December 2022

Background: An abnormally high body mass index is strongly associated with knee osteoarthritis. Usually, obese patients are excluded from clinical trials involving PRP intra-articular injections. Growth factors have been demonstrated to have a disease-modifying effect on KOA treatment, even though data on their influence on treatment effectiveness in obese patients are lacking. Purpose: To prospectively compare the level of selected growth factors including transforming growth factor-b (TGF-β), epidermal growth factor (EGF), fibroblast growth factor, insulin-like growth factor-1 (IGF-1), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), and fibroblast growth factor-2 (FGF-2) in platelet-rich plasma (PRP) in obese patients and patients with normal BMI. Methods: A total of 49 patients were included in the study according to inclusion and exclusion criteria. The groups strongly differed in body mass index (median values 21.6 vs. 32.15). Concentrations of growth factors were measured with an enzyme-linked immunosorbent assay. Statistical significance was determined with the Mann-Whitney U test. The compliance of the distribution of the results with the normal distribution was checked using the Shapiro–Wilk test separately for both groups. Results: There were no statistically significant differences in median marker levels between groups. Statistically significant Pearson correlations were observed between IGF-1 serum level and age (weak negative, r = −0.294, p = 0.041) and gender (moderate positive, r = 0.392, 0.005). Conclusions: BMI does not influence the level of selected growth factors in patients with knee osteoarthritis. Obese and non-obese patients had similar compositions of PDGF, TGF-β, EGF, FGF-2, IGF-1, and VEGF. PRP can be used in both groups with similar effects associated with growth factors’ influence on articular cartilage.
Evaluating Healthcare Performance in Low- and Middle-Income Countries: A Pilot Study on Selected Settings in Ethiopia, Tanzania, and Uganda
Authors: Belardi Paolo , Corazza Ilaria , Bonciani Manila , Manenti Fabio , Vainieri Milena
Published: December 2022

The literature reports some experiences regarding the design of integrated healthcare Performance Evaluation Systems (PES) applied in Low- and Middle-income Countries (LMIC). This study describes the design of an integrated and bottom-up PES aimed at evaluating healthcare services delivery in rural settings. The analysis involved four hospitals and their relative health districts in Ethiopia, Tanzania, and Uganda. The evaluation process was undertaken for those indicators that could be evaluated using the same reference standard. The evaluation scores were determined through the international standards identified in the literature or through benchmarking assessment. Both administrative and health data were extracted from the hospitals’ registers and District Health Information Systems (DHIS) from 2017 to 2020. We defined 128 indicators: 88 were calculated at the hospital level and 40 at the health district level. The evaluation process was undertaken for 48 indicators. The evaluated indicators are represented using effective graphical tools. In settings characterised by multiple healthcare providers, this framework may contribute to achieving good governance through performance evaluation, benchmarking, and accountability. It may promote evidence-based decision-making in the planning and allocation of resources, thus ultimately fostering quality improvement processes and practices, both at the hospital and health district level.
Understanding the Interplay between Antimicrobial Resistance, Microplastics and Xenobiotic Contaminants: A Leap towards One Health?
Authors: Piergiacomo Federica , Brusetti Lorenzo , Pagani Leonardo
Published: December 2022

According to the World Health Organization, the two major public health threats in the twenty-first century are antibiotic-resistant bacteria and antibiotic-resistant genes. The reason for the global prevalence and the constant increase of antibiotic-resistant bacteria is owed to the steady rise in overall antimicrobial consumption in several medical, domestic, agricultural, industrial, and veterinary applications, with consequent environmental release. These antibiotic residues may directly contaminate terrestrial and aquatic environments in which antibiotic-resistance genes are also present. Reports suggest that metal contamination is one of the main drivers of antimicrobial resistance (AMR). Moreover, the abundance of antibiotic-resistance genes is directly connected to the predominance of metal concentrations in the environment. In addition, microplastics have become a threat as emerging contaminants because of their ubiquitous presence, bio-inertness, toughness, danger to aquatic life, and human health implications. In the environment, microplastics and AMR are interconnected through biofilms, where genetic information (e.g., ARGs) is horizontally transferred between bacteria. From this perspective, we tried to summarize what is currently known on this topic and to propose a more effective One Health policy to tackle these threats.
Urban Vitality Measurement and Influence Mechanism Detection in China
Authors: Pan Jinghu , Zhu Xiuwei , Zhang Xin
Published: December 2022

Urban vitality is the life force of a city. In this paper, starting from three subsystems of population, economy, and function, the comprehensive index system for measuring urban vitality was constructed respectively from three scales: grid, prefecture-level administrative region, and urban agglomeration. GIS spatial analysis methods were used to measure the urban vitality index and analyze the spatial distribution pattern. Then, the MGWR was used to reveal the main factors affecting the difference in urban vitality and analyze the influence mechanism of urban vitality. Accordingly, countermeasures and suggestions for creating vibrancy were put forward. The result shows the following: At the grid scale, urban vitality presents a spatial distribution pattern of “large dispersion, small agglomeration”, which has significant differentiation characteristics of city scale and hierarchy. At the administrative region scale, the overall vitality of cities at the prefecture level and above in China is not high, and the spatial differences are large. The spatial scan identified 28 vigorous cities with high potential, belonging to 6 vigorous clusters. On the scale of urban agglomeration, according to the degree of vitality, there are three gradients. The spatial difference in urban vitality was affected by the internal characteristics and external environment.
Bedtime and the Budget: Longitudinal, Actor–Partner Connections between Sleep Quality and Financial Management Behaviors in Newlywed Couples
Authors: Saxey Matthew T. , Dew Jeffrey P. , Yorgason Jeremy B.
Published: December 2022

Research from the American Psychological Association suggests that approximately 67% of U.S. adults are getting more or less sleep than desired, and over 80% of U.S. adults ages 18–43 are stressed about finances. Cross-sectional evidence suggests that there may be a connection between the two. That is, some cross-sectional research suggests a contemporaneous association between sleep quality and finances. Using two waves of newlywed dyadic data (N = 1497 couples), we estimated a longitudinal structural equation model to test actor–partner associations between husbands’ and wives’ sleep quality and financial management behaviors. In these associations, we examined husbands’ and wives’ marital satisfaction as potential mediating variables. We found that both husbands’ and wives’ sleep quality longitudinally predicted their own and their partner’s financial management behaviors. Additionally, husbands’ and wives’ sleep quality—through wives’ marital satisfaction—indirectly and longitudinally predicted wives’ financial management behaviors. As financial practitioners encourage newlywed couples to consistently experience quality sleep, their financial management behaviors may benefit. We suggest that for newlywed couples, both partners’ bedtime may be longitudinally connected to both partners’ management of their budget.
Prediction of Asbestos-Related Diseases (ARDs) and Chrysotile Asbestos Exposure Concentrations in Asbestos-Cement (AC) Manufacturing Factories in Zimbabwe
Authors: Mutetwa Benjamin , Moyo Dingani , Brouwer Derk
Published: December 2022

The use of historical asbestos measurement data in occupational exposure assessment is essential as it allows more quantitative analysis of possible exposure response relationships in asbestos-related disease (ARD) occurrence. The aim of this study was to predict possible ARDs, namely lung cancer, mesothelioma, gastrointestinal cancer, and asbestosis, in two chrysotile asbestos cement (AC) manufacturing factories. Prediction of ARDs was done using a specific designed job-exposure matrix for airborne chrysotile asbestos fibre concentrations obtained from the Harare and Bulawayo AC factories and through application of OSHA’s linear dose effect model in which ARDs were estimated through extrapolation at 1, 10, 20, and 25 years of exposure. The results show that more cancer and asbestosis cases are likely to be experienced among those exposed before 2008 as exposure levels and subsequently cumulative exposure were generally much higher than those experienced after 2008. After a possible exposure period of 25 years, overall cancer cases predicted in the Harare factory were 325 cases per 100,000 workers, while for the Bulawayo factory, 347 cancer cases per 100,000 workers exposed may be experienced. Possible high numbers of ARDs are likely to be associated with specific tasks/job titles, e.g., saw cutting, kollergang, fettling table, ground hard waste, and possibly pipe-making operations, as cumulative exposures, though lower than reported in other studies, may present higher risk of health impairment. The study gives insights into possible ARDs, namely lung cancer, mesothelioma, gastrointestinal cancer, and asbestosis, that may be anticipated at various cumulative exposures over 1, 10, 20, and 25 years of exposure in AC manufacturing factories in Zimbabwe. Additionally, results from the study can also form a basis for more in-depth assessment of asbestos cancer morbidity studies in the AC manufacturing industries.
Does Pandemic Fatigue Prevent Farmers’ Participation in the Rural Tourism Industry: A Comparative Study between Two Chinese Villages
Authors: Qiu Mengyuan , Ni Yueli , Utomo Sulistyo
Published: December 2022

Rural tourism is an important income generation method for farmers post-pandemic. However, few studies have focused on how pandemic fatigue has affected their willingness to participate in rural tourism development. We conducted a quasi-experiment to test these effects using data from two Chinese villages. Shanlian village, which was more severely affected by COVID-19, was the experimental group, while Huashu village was set as the control group. Our results reveal that both physical and mental fatigue hinder farmers’ intention to engage in rural tourism. Further, there were significant interaction effects between physical and mental fatigue on the farmers’ participation in rural tourism. For farmers with low physical fatigue, the higher their mental fatigue, the less willing they were to participate in rural development. Conversely, for the higher physical fatigue group, farmers with low levels of mental fatigue were still more willing to participate in rural tourism development. These findings reduce the current research gap concerning the relationship between pandemic fatigue and farmers’ participation in rural tourism and indicate that practitioners and policymakers should consider farmers’ fatigue management as an important factor for the sustainability of rural tourism during the ongoing COVID-19 crisis.
Emergence of Anaplasma Species Related to A. phagocytophilum and A. platys in Senegal
Authors: Zobba Rosanna , Murgia Claudio , Dahmani Mustapha , Mediannikov Oleg , Davoust Bernard , Piredda Roberta , Schianchi Eleonora , Scagliarini Alessandra , Pittau Marco , Alberti Alberto
Published: December 2022

The genus Anaplasma (Anaplasmataceae, Rickettsiales) includes tick-transmitted bacterial species of importance to both veterinary and human medicine. Apart from the traditionally recognized six Anaplasma species (A. phagocytophilum, A. platys, A. bovis, A. ovis, A. centrale, A. marginale), novel strains and candidate species, also of relevance to veterinary and human medicine, are emerging worldwide. Although species related to the zoonotic A. platys and A. phagocytophilum have been reported in several African and European Mediterranean countries, data on the presence of these species in sub-Saharan countries are still lacking. This manuscript reports the investigation of Anaplasma strains related to zoonotic species in ruminants in Senegal by combining different molecular tests and phylogenetic approaches. The results demonstrated a recent introduction of Candidatus (Ca) Anaplasma turritanum, a species related to the pathogenic A. platys, possibly originating by founder effect. Further, novel undetected strains related to Candidatus (Ca) Anaplasma cinensis were detected in cattle. Based on groEL and gltA molecular comparisons, we propose including these latter strains into the Candidatus (Ca) Anaplasma africanum species. Finally, we also report the emergence of Candidatus (Ca) A. boleense in Senegal. Collectively, results confirm that Anaplasma species diversity is greater than expected and should be further investigated, and that Anaplasma routine diagnostic procedures and epidemiological surveillance should take into account specificity issues raised by the presence of these novel strains, suggesting the use of a One Health approach for the management of Anaplasmataceae in sub-Saharan Africa.
Memory Macrophages
Authors: Kloc Malgorzata , Kubiak Jacek Z. , Zdanowski Robert , Ghobrial Rafik M.
Published: December 2022

Immunological memory is a crucial part of the immune defense that allows organisms to respond against previously encountered pathogens or other harmful factors. Immunological memory is based on the establishment of epigenetic modifications of the genome. The ability to memorize encounters with pathogens and other harmful factors and mount enhanced defense upon subsequent encounters is an evolutionarily ancient mechanism operating in all animals and plants. However, the term immunological memory is usually restricted to the organisms (invertebrates and vertebrates) possessing the immune system. The mammalian immune system, with innate and adaptive branches, is the most sophisticated among vertebrates. The concept of innate memory and memory macrophages is relatively new and thus understudied. We introduce the concept of immunological memory and describe types of memory in different species and their evolutionary status. We discuss why the traditional view of innate immune cells as the first-line defenders is too restrictive and how the innate immune cells can accumulate and retain immunologic memory. We describe how the initial priming leads to chromatin remodeling and epigenetic changes, which allow memory macrophage formation. We also summarize what is currently known about the mechanisms underlying development of memory macrophages; their molecular and metabolic signature and surface markers; and how they may contribute to immune defense, diseases, and organ transplantation.
Circulating miRNA Expression Profiles and Machine Learning Models in Association with Response to Irinotecan-Based Treatment in Metastatic Colorectal Cancer
Authors: Pliakou Evangelia , Lampropoulou Dimitra Ioanna , Dovrolis Nikolas , Chrysikos Dimosthenis , Filippou Dimitrios , Papadimitriou Christos , Vezakis Antonios , Aravantinos Gerasimos , Gazouli Maria
Published: December 2022

Colorectal cancer represents a leading cause of cancer-related morbidity and mortality. Despite improvements, chemotherapy remains the backbone of colorectal cancer treatment. The aim of this study is to investigate the variation of circulating microRNA expression profiles and the response to irinotecan-based treatment in metastatic colorectal cancer and to identify relevant target genes and molecular functions. Serum samples from 95 metastatic colorectal cancer patients were analyzed. The microRNA expression was tested with a NucleoSpin miRNA kit (Machnery-Nagel, Germany), and a machine learning approach was subsequently applied for microRNA profiling. The top 10 upregulated microRNAs in the non-responders group were hsa-miR-181b-5p, hsa-miR-10b-5p, hsa-let-7f-5p, hsa-miR-181a-5p, hsa-miR-181d-5p, hsa-miR-301a-3p, hsa-miR-92a-3p, hsa-miR-155-5p, hsa-miR-30c-5p, and hsa-let-7i-5p. Similarly, the top 10 downregulated microRNAs were hsa-let-7d-5p, hsa-let-7c-5p, hsa-miR-215-5p, hsa-miR-143-3p, hsa-let-7a-5p, hsa-miR-10a-5p, hsa-miR-142-5p, hsa-miR-148a-3p, hsa-miR-122-5p, and hsa-miR-17-5p. The upregulation of microRNAs in the miR-181 family and the downregulation of those in the let-7 family appear to be mostly involved with non-responsiveness to irinotecan-based treatment.
Porcine Relaxin but Not Serelaxin Shows Residual Bioactivity after In Vitro Simulated Intestinal Digestion—Clues for the Development of New Relaxin Peptide Agonists Suitable for Oral Delivery
Authors: Pacini Lorenzo , D’Ercole Annunziata , Papini Anna Maria , Bani Daniele , Nistri Silvia , Rovero Paolo
Published: December 2022

Despite human recombinant H2 relaxin or serelaxin holding promise as a cardiovascular drug, its actual efficacy in chronic treatment of heart failure patients was hampered by the need to be administered by multiple daily IV injections for a long time, with obvious drawbacks in terms of patients’ compliance. This in vitro study aimed at exploring the molecular background for a possible administration of the peptide hormone relaxin by the oral route. Serelaxin and purified porcine relaxin (pRLX) were subjected to simulated intestinal fluid (SIF) enzymatic digestion in vitro to mimic the behavior of gastroprotective formulations. The digestion time course was studied by HPLC, and the relative bio-potency of the intact molecules and their proteolytic fragments was assessed by second messenger (cAMP) response in RXFP1 relaxin receptor-bearing THP-1 human monocytic cells. Both intact proteins (100 ng/mL) induced a significant cAMP rise in THP-1 cells. Conversely, SIF-treated serelaxin showed a brisk (30 s) bioactivity decay, dropping down to the levels of the unstimulated controls at 120 s, whereas SIF-treated pRLX retained significant bioactivity for up to 120 s. After that, it progressively declined to the levels of the unstimulated controls. HPLC analysis indicates that this bioactivity could be ascribed to a minor component of the pRLX sample more resistant to proteolysis. When identified and better characterized, this peptide could be exploited for the development of synthetic relaxin agonists suitable for oral formulations.
The Role of Mononuclear Phagocytes in the Testes and Epididymis
Authors: Shi Xu , Zhao Hu , Kang Yafei , Dong Xinyi , Yu Caiqian , Xie Qinying , Duan Yonggang , Liao Aihua , Huang Donghui
Published: December 2022

The mononuclear phagocytic system (MPS) is the primary innate immune cell group in male reproductive tissues, maintaining the balance of pro-inflammatory and immune tolerance. This article aims to outline the role of mononuclear macrophages in the immune balance of the testes and epididymis, and to understand the inner immune regulation mechanism. A review of pertinent publications was performed using the PubMed and Google Scholar databases on all articles published prior to January 2021. Search terms were based on the following keywords: ‘MPS’, ‘mononuclear phagocytes’, ‘testes’, ‘epididymis’, ‘macrophage’, ‘Mφ’, ‘dendritic cell’, ‘DC’, ‘TLR’, ‘immune’, ‘inflammation’, and ‘polarization’. Additionally, reference lists of primary and review articles were reviewed for other publications of relevance. This review concluded that MPS exhibits a precise balance in the male reproductive system. In the testes, MPS cells are mainly suppressed subtypes (M2 and cDC2) under physiological conditions, which maintain the local immune tolerance. Under pathological conditions, MPS cells will transform into M1 and cDC1, producing various cytokines, and will activate T cell specific immunity as defense to foreign pathogens or self-antigens. In the epididymis, MPS cells vary in the different segments, which express immune tolerance in the caput and pro-inflammatory condition in the cauda. Collectively, MPS is the control point for maintaining the immune tolerance of the testes and epididymis as well as for eliminating pathogens.
Alpha-Lipoic Acid and Its Enantiomers Prevent Methemoglobin Formation and DNA Damage Induced by Dapsone Hydroxylamine: Molecular Mechanism and Antioxidant Action
Authors: Espíndola Kaio Murilo Monteiro , Varela Everton Luiz Pompeu , de Albuquerque Rosyana de Fátima Vieira , Figueiredo Rosiane Araújo , dos Santos Sávio Monteiro , Malcher Nívea Silva , da S. Seabra Pamela Suelen , Fonseca Andréia do Nascimento , de Azevedo Sousa Karla Marcely , de Oliveira Susan Beatriz Batista , Carneiro Agnaldo da Silva , Coleman Michael D. , Monteiro Marta Chagas
Published: December 2022

Dapsone (DDS) therapy can frequently lead to hematological side effects, such as methemoglobinemia and DNA damage. In this study, we aim to evaluate the protective effect of racemic alpha lipoic acid (ALA) and its enantiomers on methemoglobin induction. The pre- and post-treatment of erythrocytes with ALA, ALA isomers, or MB (methylene blue), and treatment with DDS-NOH (apsone hydroxylamine) was performed to assess the protective and inhibiting effect on methemoglobin (MetHb) formation. Methemoglobin percentage and DNA damage caused by dapsone and its metabolites were also determined by the comet assay. We also evaluated oxidative parameters such as SOD, GSH, TEAC (Trolox equivalent antioxidant capacity) and MDA (malondialdehyde). In pretreatment, ALA showed the best protector effect in 2.5 µg/mL of DDS-NOH. ALA (1000 µM) was able to inhibit the induced MetHb formation even at the highest concentrations of DDS-NOH. All ALA tested concentrations (100 and 1000 µM) were able to inhibit ROS and CAT activity, and induced increases in GSH production. ALA also showed an effect on DNA damage induced by DDS-NOH (2.5 µg/mL). Both isomers were able to inhibit MetHb formation and the S-ALA was able to elevate GSH levels by stimulating the production of this antioxidant. In post-treatment with the R-ALA, this enantiomer inhibited MetHb formation and increased GSH levels. The pretreatment with R-ALA or S-ALA prevented the increase in SOD and decrease in TEAC, while R-ALA decreased the levels of MDA; and this pretreatment with R-ALA or S-ALA showed the effect of ALA enantiomers on DNA damage. These data show that ALA can be used in future therapies in patients who use dapsone chronically, including leprosy patients.