bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Polymorphism of the β1 Adrenoreceptor and CYP2D6 on the Therapeutic Effects of Metoprolol
Authors: Yuan H , Huang Z , Yang G , Lv H , Sang H , Yao Y
Published: February 2013

Metoprolol is a lipophilic β1 selective adrenergic receptor antagonist used in antihypertensive therapy. It is metabolized by the protein product of the cytochrome P450 2D6 ( CYP2D6) gene. Few studies have been performed on the association between the β1 adrenoreceptor, CYP2D6 polymorphism and blood pressure. The one reported here is a prospective, observational, clinical study in Chinese Han hypertensive patients on the combined influences of CYP2D6 and β1 adrenoreceptor polymorphisms on the therapeutic effects of metoprolol in 300 out-patients with essential hypertension. The same dose of metoprolol achieved different therapeutic effects in patients with different CYP2D6 and β1 adrenoreceptor polymorphisms. Additionally, different doses of metoprolol could achieve the same therapeutic effects in patients with different CYP2D6 and β1 adrenoreceptor polymorphisms. Knowledge of the combination of CYP2D6 and β1 adrenoreceptor polymorphisms may be used to guide antihypertensive therapy using β1 adrenoreceptor antagonists.
Infrared Thermography Based on Artificial Intelligence for Carpal Tunnel Syndrome Diagnosis
Authors: Papež B Jesenšek , Palfy M , Turk Z
Published: February 2013

Thermography for the measurement of surface temperatures is well known in industry, although is not established in medicine despite its safety, lack of pain and invasiveness, easy reproducibility, and low running costs. Promising results have been achieved in nerve entrapment syndromes, although thermography has never represented a real alternative to electromyography. Here an attempt is described to improve the diagnosis of carpal tunnel syndrome with thermography using a computer-based system employing artificial neural networks to analyse the images. Method reliability was tested on 112 images (depicting the dorsal and palmar sides of 26 healthy and 30 pathological hands), with the hand divided into 12 segments and compared relative to a reference. Palmar segments appeared to have no beneficial influence on classification outcome, whereas dorsal segments gave improved outcome with classification success rates near to or over 80%, and finger segments influenced by the median nerve appeared to be of greatest importance. These are preliminary results from a limited number of images and further research will be undertaken as our image database grows.
Plasma Ghrelin Levels and Weight Loss in Chinese Uygur Patients with Chronic Obstructive Pulmonary Disease
Authors: Ying BW , Song XB , Fan H , Wang LL , Li YS , Cheng Z , Cheng H , Wen FQ
Published: February 2013

Ghrelin is an important orexigenic hormone that reduces fat oxidation and increases adiposity. This study investigated plasma ghrelin levels in Chinese Uygur patients with chronic obstructive pulmonary disease (COPD). Plasma ghrelin and anabolic and catabolic factors were measured in 38 patients and 24 control subjects. COPD patients were divided into two groups based on body mass index (BMI): underweight (BMI ≤ 20 kg/m2, n = 18) or normoweight (BMI ≤ 20 kg/m2, n = 20). Plasma ghrelin levels were found to be significantly higher in underweight than in normoweight patients or healthy controls. Circulating tumour necrosis factor-α and interleukin-6 concentrations were significantly higher in underweight than in normoweight patients, whereas insulin concentrations were significantly lower. Plasma ghrelin levels correlated negatively with forced expiratory volume in 1 s (FEV1; r = 0.35), but did not significantly correlate with FEV1/forced vital capacity. Plasma ghrelin levels were elevated in underweight COPD patients and were associated with cachexia and abnormal pulmonary function.
Detection of Coagulase Activity in Pathogenic Candida Species
Authors: Yigit N , Aktas AE , Ayyildiz A
Published: February 2013

The coagulase activities of various Candida spp were assessed using a classical tube test. A total of 125 clinical Candida isolates representing eight species ( C. albicans, C. parapsilosis, C. tropicalis, C. glabrata, C. kefyr, Geotrichum candidum, C. krusei and C. guilliermondii) were incubated with rabbit, sheep or human plasma. After 24 h incubation, 29/64 (45.3%) C. albicans isolates and 23/61 (37.7%) non-albicans Candida strains were positive for coagulase in rabbit plasma, whereas 20/64 (31.2%) C. albicans and 6/61 (9.8%) non-albicans Candida isolates were positive for coagulase in sheep plasma. None of the tested Candida isolates reacted positively in human plasma. These results suggest that rabbit plasma is the most appropriate medium for detection of coagulase activity in Candida spp. Variations in coagulase production by Candida spp may be related to their pathogenicity. It is concluded that the laboratory detection of coagulase activity in clinical isolates of Candida spp may help the diagnosis of Candida-related infections.
Primary Amelanotic Malignant Melanoma of the Parotid Gland: A Case Report
Authors: Gao N , Li L-J , Li Y , Wang L
Published: February 2013

Amelanotic malignant melanoma in the oral region is extremely rare and has not previously been reported in the parotid gland. This present case report describes an amelanotic malignant melanoma in the parotid gland, with no other primary lesion detectable. The medical history, pathology and immunohistochemical analysis of the case are described and the relevant literature is reviewed in order to help in the recognition of this uncommon tumour. Amelanotic malignant melanoma is a melanoma subtype with little or no pigmentation and, because of this lack of pigmentation and its wide-ranging clinical appearance, it often defies clinical diagnosis. A high level of vigilance is, therefore, necessary in diagnosing such a tumour in the parotid gland. Where it is suspected, positive expression of S-100 protein and human melanoma black 45 (HMB45) using immunohistochemical analysis can be considered reliable methods of confirming diagnosis.
Clear Lens Phacoemulsification with Continuous Curvilinear Capsulorhexis and Foldable Intraocular Lens Implantation for the Treatment of a Patient with Bilateral Anterior Lenticonus due to Alport Syndrome
Authors: Liu YB , Tan SJ , Sun ZY , Li X , Huang BY , Hu QM
Published: February 2013

The case of a 29-year-old man is reported who presented with a gradually progressive, painless decrease in vision over 10 years. Anterior segment examination with a slit lamp revealed anterior lenticonus in both eyes. The patient had previously been diagnosed with bilateral sensorineural deafness, however investigations revealed microscopic haematuria and renal insufficiency that subsequently led to a diagnosis of classical Alport syndrome. Since the patient's quality of vision was severely affected by the bulging anterior lens capsule, surgical treatment was required. Clear lens phacoemulsification with continuous curvilinear capsulorhexis and foldable intraocular lens implantation were performed in each eye 2 days apart. One week after surgery, visual acuity was excellent in both eyes. Clear lens phacoemulsification with continuous curvilinear capsulorhexis and foldable intraocular lens implantation was a safe and effective therapeutic choice in this patient for the management of anterior lenticonus due to Alport syndrome.
Probiotics in Clinical Practice: An Overview
Authors: Zuccotti GV , Meneghin F , Raimondi C , Dilillo D , Agostoni C , Riva E , Giovannini M
Published: February 2013

The observation that intestinal bacterial microflora might be able to influence immune system surveillance through changed nutritional habits has raised awareness of the role of probiotics. These are live microorganisms that are able to reach the gastrointestinal tract and alter its microfloral composition, producing beneficial health effects when consumed in adequate amounts. Recent clinical trials have evaluated the clinical effectiveness of probiotics in the treatment and prevention of a wide range of acute and chronic gastrointestinal diseases, and also non-gastrointestinal diseases, such as atopy, respiratory infections, vaginitis and hypercholesterolaemia. Probiotic supplements are generally regarded as safe because the microorganisms they contain are identical to those found in human gastrointestinal and vaginal microflora. Guidelines on the use of probiotics in the clinical setting require periodical updates for the latest data to be included in clinical applications. The purpose of this clinical report is to review current evidence on the use of probiotics in a variety of gastrointestinal and non-gastrointestinal conditions.
Mesenchymal Stem Cells Genetically Modified with the Angiopoietin-1 Gene Enhanced Arteriogenesis in a Porcine Model of Chronic Myocardial Ischaemia
Authors: Chen SL , Zhu CC , Liu YQ , Tang LJ , Yi L , Yu BJ , Wang DJ
Published: February 2013

The direct injection by thoracoscope of mesenchymal stem cells (MSCs) that had been genetically modified to express angiopoietin-1 was investigated in a porcine model to determine their effect on arteriogenesis and the effectiveness of this technique. Chronic myocardial ischaemia was established using a thoracoscope to insert an ameroid constrictor around the left circumflex coronary artery. Six weeks after establishing the ischaemia, 20 pigs were randomly divided into three groups to receive injections by thoracoscope of either genetically-modified MSCs, unmodified MSCs or phosphate-buffered saline into the ischaemic border area. The injections were repeated 1 month later. The genetically modified MSCs were found to restore blood flow significantly more than the other observed treatments and immunohistochemical evaluation of arteriogenesis supported this finding. In conclusion, the injection of MSCs that had been genetically modified to express angiopoietin-1 improved arteriogenesis and increased collateral blood flow in the myocardial ischaemic area. Thoracoscope delivery of the injection was safe and minimally invasive.
How to Increase the Diagnostic Value of Malignancy-Related Ascites: Discriminative Ability of the Ascitic Tumour Markers
Authors: Tuzun Y , Yilmaz Ş , Dursun M , Canoruc F , Çelik Y , Çil T , Boyraz T
Published: February 2013

Making a differential diagnosis between malignant and non-malignant ascites is an important clinical issue, but cytological examination has a relatively low diagnostic sensitivity. This study aimed to find a discriminative model that distinguished between malignancy-related and non-malignant ascites. The study included 107 patients: 50 with non-malignant and 57 with malignant ascites. Ascites was analysed using a range of tumour markers and standard cytology. Standardized canonical discriminant function coefficients were used to distinguish between ascites types. The combination of carbohydrate antigen (CA) 15-3, carcinoembryonic antigen (CEA) and cytokeratin 19 fragments (CYFRA-21.1) discriminated between malignancy-related ascites and non-malignant ascites with an accuracy of 98.8% compared with an accuracy of 77.8% for cytological examination. In conclusion, the use of a discriminant function constructed from a combination of CA15-3, CEA and CYFRA-21.1 could distinguish malignant from non-malignant ascites with greater accuracy than cytological examination. Further studies in larger population groups are warranted.
The Effects of Open Lung Ventilation on Respiratory Mechanics and Haemodynamics in Atelectatic Infants after Cardiopulmonary Bypass
Authors: Cui Q , Zhou H , Zhao R , Liu J , Yang X , Zhu H , Zheng Q , Gu C , Yi D
Published: February 2013

Acute lung injury (ALI) frequently occurs in infants after cardiopulmonary bypass (CPB) surgery and it sometimes develops into acute respiratory distress syndrome in critically ill infants, which can be life threatening. This study investigated the effects of open lung ventilation on the haemodynamics and respiratory mechanics of 64 infants (34 males; 30 females) with a mean ± SD age of 8.3 ± 0.3 months who developed ALI following CPB surgery. Open lung ventilation significantly improved the respiratory mechanics and oxygenation parameters of the infants, including the partial pressure of oxygen in arterial blood (PaO2), the ratio of PaO2/FiO2 (fraction of inspired oxygen), peak inspiratory pressure, static compliance and airway resistance. It is concluded that open lung ventilation can greatly improve oxygenation and respiratory mechanics in infants with ALI following CPB surgery.
Correlation of Slime Production Investigated via Three Different Methods in Coagulase-Negative Staphylococci with Crystal Violet Reaction and Antimicrobial Resistance
Authors: Bozkurt H , Kurtoglu MG , Bayram Y , Keşli R , Berktaş M
Published: February 2013

This study investigated slime production by coagulase-negative staphylococci (CNS) using the standard tube (ST), Congo red agar (CRA) plate and Christensen's tube (CT) methods, and compared the results with those of the crystal violet reaction (CVR) test. The potential correlation between slime production and antimicrobial resistance was also evaluated. In total, 205 CNS strains were isolated from biological samples: 92 (44.9%) were shown to produce slime by the ST method; 96 (46.8%) by the CRA plate method; 90 (43.9%) by the CT method; and 89 (43.4%) strains were CVR positive. Eighty-three (40.5%) CNS strains were positive for slime production by the ST, CRA and CT methods. The findings of the ST, CRA and CT test methods were consistent with each other but were not related to CVR positivity. Based on the ST method, rates of antibiotic resistance to several antimicrobial agents were higher in slime-positive strains than in slime-negative strains and, in some cases, this was statistically significant.
Stromal Cell-Derived Factor-1 and Vascular Endothelial Growth Factor May Play an Important Role in the Process of Neovascularization of Herniated Intervertebral Discs
Authors: Jia C-Q , Zhao J-G , Zhang S-F , Qi F
Published: February 2013

Increased levels of stromal cell-derived factor (SDF)-1 occur in certain pathological situations, however the relationship between SDF-1α expression and human intervertebral disc herniation is unknown. Vascular endothelial growth factor (VEGF) plays an essential role in the formation of new blood vessels. The present study used immunohistochemistry to measure the expression of SDF-1α and VEGF in surgical specimens of human intervertebral discs to evaluate their role in neovascularization. The frequency of expression of SDF-1α and VEGF was found to be significantly higher in extruded and sequestrated herniated intervertebral discs compared with bulging, protruding and prolapsed herniated discs and control discs and there was high correlation between SDF-1α and VEGF expressions. It is concluded that SDF-1α and VEGF are expressed in human intervertebral discs and their interaction may be important in accumulating endothelial progenitor cells during the neovascularization processes in herniated intervertebral discs.
The Renal Protective Effects of Cilostazol on Suppressing Pathogenic Thrombospondin-1 and Transforming Growth Factor-β Expression in Streptozotocin-Induced Diabetic Rats
Authors: Wang X , Yan L , Chen W , Xu L , Zhang X
Published: February 2013

Diabetic nephropathy is a major complication facing patients with diabetes mellitus. The renal protective effects of the phosphodiesterase III inhibitor, cilostazol, were investigated in rats with streptozotocin-induced diabetes. Expression of thrombospondin-1 (TSP-1) and transforming growth factor-β (TGF-β) in the kidney was measured by immunohistochemistry and real-time reverse transcription-quantitative polymerase chain reaction analysis in diabetic rats, cilostazol-treated diabetic rats and control rats. Ultrastructural changes in the kidney were also analysed using microscopy. Four weeks after the induction of diabetes, TSP-1 and TGF-β expression was significantly increased in the kidneys of diabetic rats compared with the control and was significantly lower in cilostazol-treated diabetic rats than in the untreated diabetic rats. Microscopy revealed characteristic renal pathology in the diabetic group, which was rarely seen in the cilostazol-treated diabetic rats. In conclusion, this study indicates that cilostazol treatment of diabetic rats effectively prevents pathological kidney changes, possibly via the down-regulation of TSP-1 and TGF-β expression compared with untreated rats.
E-Cadherin Expression in Endometrial Malignancies: Comparison between Endometrioid and Non-Endometrioid Carcinomas
Authors: Yalta T , Atay L , Atalay F , Çaydere M , Gonultas M , Ustun H
Published: February 2013

This study examined the frequency of E-cadherin expression in endometrial biopsy or hysterectomy specimens from patients diagnosed with endometrial adenocarcinoma and in normal endometrial tissue specimens. E-cadherin expression was detected by immunohistochemistry using monoclonal antibody to E-cadherin. Specimens were classified as positive when ≥ 5% of the tumour cells showed staining for E-cadherin, irrespective of the pattern of staining. Twenty-three endometrioid carcinomas and nine non-endometrioid (four papillary serous and five clear cell) carcinomas were studied, along with 10 normal endometrial tissue specimens as controls. E-cadherin expression was significantly less frequent in non-endometrioid carcinomas compared with endometrioid carcinomas and controls. There was no statistically significant difference in the frequency of E-cadherin expression between endometrioid carcinomas and controls. In conclusion, this study demonstrated that uterine non-endometrioid (papillary serous and clear cell) carcinomas were less likely to express E-cadherin compared with endometrioid carcinomas and normal endometrial tissue. This may help to explain the more aggressive behaviour of non-endometrioid carcinomas.
Clinical Analysis of Head and Neck Cancer Cases in South-West China 1953–2002
Authors: Gao N , Li Y , Li L-J , Wen Y-M
Published: February 2013

The occurrence of head and neck cancers varies worldwide. This study retrospectively analysed the incidence and types of 8646 cases of head and neck cancers in south-west China that were treated at the West China Stomatology Hospital of Sichuan University between 1953 and 2002. Overall mean patient age was 50.3 years and the overall male:female ratio was 2.38:1; mean age increased and the male:female ratio decreased over the study period. Peak incidence occurred between the ages of 40 and 60 years. Primary tumours most frequently developed in the tongue, followed by the bucca and gingiva. Histologically, squamous cell carcinomas were most frequently recorded. The parotid gland and palate were the most common locations for salivary gland tumours. Over the study period the incidence of head and neck cancers increased with time and the rate of increase was greater in females than males. The frequency of histological types and topography were similar to previous reports.
Possible Involvement of Brain Tumour Stem Cells in the Emergence of a Fast-Growing Malignant Meningioma after Surgical Resection and Radiotherapy of High-Grade Astrocytoma: Case Report and Preliminary Laboratory Investigation
Authors: Hu W , Shen F , Chen G , Shen G , Liu W , Zhou J
Published: February 2013

The case of a 62-year old man diagnosed with radiation-induced meningioma (RIM) after treatment for astrocytoma with an unusually short latency period of 7 months is reported. The patient first presented with a 2-month history of memory decline. Magnetic resonance imaging (MRI) showed a tumour in the left parieto-temporal lobe. Gross total resection was performed and the tumour was confirmed to be an astrocytoma. The patient received cranial radiotherapy 2 weeks later, however 7 months after radiation treatment the patient presented with headache and vomiting. MRI showed massive meningeal enhancement in the left frontal lobe, which progressively enlarged. The patient's clinical condition deteriorated and a second craniotomy was performed with complete removal of the secondary tumour, which was shown to be a malignant meningioma. Immunohistochemical staining identified CD133-positive cells in both tumours. A rare fraction of brain tumour stem cells (BTSC) was isolated from the primary astrocytoma using a serum-free culture system, suggesting that BTSC may have been involved in the rapid emergence of RIM after resection and radiation of the primary astrocytoma.
Acute Urinary Retention Secondary to a Urethral Calculus in a Bladder-Drained Kidney Pancreas Transplant Patient – a Metallic Clip Nidus
Authors: Smith JBK , Sairam K , Olsburgh J
Published: February 2013

Urological expertise is usually required for the management of any urological complications of bladder-drained pancreatic allografts whether they are the result of simultaneous pancreas–kidney transplants, pancreas after kidney transplants, or pancreas transplants alone. This study presents a case of urinary retention secondary to prostatic urethra calculus impaction, the nidus of which was found to be metallic staples from the donor duodenal segment of a pancreatic allograft. Knowledge of the pre-transplant benchwork gave a high index of suspicion to the urological sequelae of this case and, in particular, the presence of calculi should suggest a metal clip nidus. We examine the methods of exocrine pancreatic drainage, donor duodenum preparation and case management.
Co-Existent de novo Myelodysplastic Syndrome and T-cell Non-Hodgkin Lymphoma: a Common Origin or Not?
Authors: Huang H-H , Zhu J-Y , Han J-Y , Shen L-J , Xu L , Chen X-L , Zheng L-Y , Chen F-Y
Published: February 2013

The co-existence of de novo myelodysplastic syndrome (MDS) and non-Hodgkin lymphoma (NHL) prior to therapy is an extremely unusual finding. We report here a case of co-existent de novo MDS-refractory cytopenia with multilineage dysplasia and T-cell NHL, including clinical features, histopathological findings, molecular assessment, treatment course and outcomes. Other cases from the literature showing co-existence of both disorders are also reviewed; to date 19 similar cases have been reported. Among all cases (including the present patient), eight cases were diagnosed with de novo MDS and NHL simultaneously, which were considered to be true coincidences. The mechanisms responsible for the appearance of co-existence have not yet been ascertained, however in the present case a common chromosomal abnormality (20q deletion) was found in bone marrow and lymph node preparations. We conclude, therefore, that the co-existent de novo MDS and T-cell NHL seen in the present case may have a common origin.
Efficacy and Safety of Valsartan/Hydrochlorothiazide Fixed-dose Combination Compared with Amlodipine Monotherapy as First-line Therapy for Mild to Moderate Hypertension
Authors: Chao CL , Lin YH , Lin LC , Lin LY , Tsai CT , Wang YC , Hwang JJ , Chen JC , Chiang FT
Published: February 2013

This double-blind, active- and randomized-controlled study compared the efficacy and safety of a fixed-dose combination of valsartan/hydrochlorothiazide 80 mg/12.5 mg once daily ( n = 32) with amlodipine monotherapy 5 mg once daily ( n = 33) for 8 weeks in patients with mild to moderate hypertension. Non-inferiority of valsartan/hydrochlorothiazide to amlodipine was demonstrated by comparable reductions in sitting systolic blood pressure (SBP), sitting diastolic blood pressure (DBP), and daytime, night-time and 24-h SBP and DBP on ambulatory blood pressure monitoring. Between-group comparisons of adverse events and changes in laboratory parameters did not reach statistical significance, except for uric acid which showed a significant increase in the valsartan/hydrochlorothiazide group compared with the amlodipine group, but was still below the laboratory's upper limit of normal. In conclusion, the use of the fixed-dose combination of valsartan/hydrochlorothiazide 80 mg/12.5 mg once daily as a starting regimen in patients with mild to moderate hypertension was shown to have non-inferior efficacy and comparable safety for daily practice compared with amlodipine 5 mg once daily monotherapy.
Obstructive Sleep Apnoea, Cigarette Smoking and Plasma Orexin-A in a Sleep Clinic Cohort
Authors: Aksu K , Güven S Firat , Aksu F , Ciftci B , Ciftci T Ulukavak , Aksaray S , Şipit T , Peker Y
Published: February 2013

Orexin-A is a neuropeptide involved in the regulation of food intake and the sleep-wake cycle. This study investigated plasma orexin-A levels in a sleep clinic cohort, adjusting for smoking habits, in 76 participants comprising 41 with obstructive sleep apnoea (OSA) (apnoea-hypopnoea index [AHI] 44.1 ± 19.1 events/h) and 35 without OSA (AHI 6.3 ± 4.7 events/h). Plasma orexin-A levels were significantly lower in OSA patients (15.0 ± 4.6 ng/ml) compared with those without OSA (31.4 ± 6.5 ng/ml). In non-OSA subjects, there was no significant difference between never smokers and ex/current smokers in plasma orexin-A levels (32.9 ± 9.5 versus 29.7 ± 8.9 ng/ml, respectively) whereas, in the OSA sub-group, orexin-A levels were significantly lower in never smokers than in ex/current smokers (4.0 ± 1.2 versus 21.4 ± 7.0 ng/ml). A significant inverse relationship was found between plasma orexin-A levels and AHI amongst never smokers, but there was no significant relationship amongst ex/current smokers. These results confirm previous studies demonstrating lower levels of plasma orexin-A in OSA patients and indicate that smoking may affect orexin-A levels and AHI.
Clinicopathological Variables Associated with Lymph Node Metastasis and Prognostic Factors in pT2 Gastric Cancer
Authors: Wang A , Guo P , Sun Z , Xu H
Published: February 2013

This retrospective study investigated the clinicopathological variables associated with lymph node metastasis and prognosis in 325 patients with pT2 gastric cancer in order to set out a foundation for the surgical management of this condition. Univariate and multivariate analyses identified factors that were predictive of lymph node metastasis. Cox regression analysis evaluated the effect of prognostic factors on survival. Tumour location, maximum tumour diameter, lymphatic invasion and total retrieved lymph nodes (tLN) were revealed as independent factors for lymph node metastasis in pT2a gastric cancer, whereas histological type, lymphatic invasion and tLN were associated with lymph node metastasis in patients with pT2b gastric cancer. Maximum tumour diameter, lymphatic invasion and metastatic lymph node (mLN) ratio, but not tLN, were independent prognostic factors in pT2a cancer. Maximum tumour diameter and mLN ratio, but not tLN, were independent prognostic factors in pT2b cancer. Overall, it is concluded that maximum tumour diameter and mLN ratio are important prognostic factors in pT2 gastric cancer.
Prediction of Functional Outcome in Stunned Myocardium after Myocardial Infarction using BMIPP and Tetrofosmin Imaging
Authors: Ueda T , Toyama T , Seki R , Hoshizaki H , Oshima S , Taniguchi K , Endo K , Kurabayashi M
Published: February 2013

The predictive value of combined 123iodine-labelled 15-( p-iodophenyl)-3 R,S-methyl pentadecanoic acid imaging (123I-BMIPP) and early technetium-99m (99mTc)-tetrofosmin imaging was compared with combined 123I-BMIPP and delayed 99mTc-tetrofosmin or 123I-BMIPP and thallium-201 (201Tl) imaging for functional outcome of stunned myocardium after acute myocardial infarction (AMI) in 37 patients with AMI. All patients underwent successful percutaneous coronary intervention with/without stenting within 24 h of symptoms. Resting 201Tl, 99mTc-tetrofosmin and 123I-BMIPP imaging were performed within 10 days of hospital admission; 99mTc-tetrofosmin imaging was also performed 6 months later. Segments were mismatched when the 123I-BMIPP score was greater than the 99mTc-tetrofosmin or 201Tl scores, and were matched when all scores were the same. Left ventricular function was estimated using wall motion score. Sensitivity and regional wall motion were significantly better in mismatching 99mTc-tetrofosmin-early/123I-BMIPP segments than mismatching 201Tl/123I-BMIPP or 99mTc-tetrofosmin-delayed/123I-BMIPP segments. It is concluded that mismatching of 123I-BMIPP and early 99mTc-tetrofosmin uptake can predict improvement in wall motion of stunned myocardium better than the other two imaging combinations.
Impact of Cystic Fibrosis Transmembrane Conductance Regulator Gene Mutation on the Occurrence of Chronic Pancreatitis in Japanese Patients
Authors: Aoyagi H , Okada T , Hasatani K , Mibayashi H , Hayashi Y , Tsuji S , Kaneko Y , Yamagishi M
Published: February 2013

DNA analyses of the cystic fibrosis transmembrane conductance regulator ( CFTR) gene in Japanese patients with idiopathic chronic pancreatitis (ICP) were performed to determine the relationship between the CFTR mutation and ICP. The study included patients with alcoholic pancreatitis ( n = 20), patients with ICP ( n = 20) and healthy volunteers (controls; n = 110). The poly-T region in intron 8 of the CFTR gene was analysed by direct sequencing. The CFTR coding region was screened using single-strand conformational polymorphism and direct sequencing. In the controls, frequencies of the 5T genotype and 5T allele were 4.5% and 3.6%, respectively. The frequency of the 5T genotype was significantly higher in the ICP group (20%) versus controls, but was not significantly different in alcoholic chronic pancreatitis patients (5%). Thus, the CFTR gene mutation, especially the 5T genotype, appears to have some relationship to ICP prevalence in Japanese patients independent of cystic fibrosis.
Human Cytomegalovirus Glycoprotein Genotypes in the Genital Tract Tissue of Tubal Pregnancy Patients
Authors: Qian H-L , Cai T , Jin H-M
Published: February 2013

This study investigated human cytomegalovirus (HCMV) glycoprotein genotypes in the genital tract tissue of 125 tubal pregnancy patients. The HCMV glycoprotein-B N-terminus (gBn, 54 − 485 NT), gB endoprotease cleavage site (gBclv, 1284 − 1600 NT) and glycoprotein-H (gH, −58 − 213 NT) gene fragments were amplified by nested polymerase chain reaction and sequenced to identify gB and gH genotypes. Of 16 gBn-positive samples, four were gBn1, one was gBn2 and 11 were the gBn3 genotype. Of 13 positive gBclv samples, seven were gBclv1, two were gBclv2 and four were the gBclv3 genotype. Of 20 positive gH samples, 10 were gH1, six were gH2 and four were a combined gH1/gH2 genotype. In 10 of the samples that were positive for the gBn and gBclv genotypes, the gBn and gBclv genotypes were not consistent (four were gBclv1-gBn3). This study showed that: (i) HCMV infection with the gB1–gB3 glycoprotein genotypes is present in tubal pregnancy; (ii) the gBclv and gBn genotypes are not strictly consistent; and (iii) intragenetic variability within the gB gene due to homologous recombination occurs frequently.
Effects of Alendronate on the Proliferation and Osteogenic Differentiation of MG-63 Cells
Authors: Xiong Y , Yang HJ , Feng J , Shi ZL , Wu LD
Published: February 2013

Previous studies of the direct actions of bisphosphonates on bone have mainly been limited to their effects on bone-resorbing osteoclasts and little is known about the direct effects of bisphosphonates on osteoblasts. Here we report the direct effects of alendronate on the proliferation and osteogenic differentiation of the MG-63 osteoblast-like cell line. Cell proliferation was determined with the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay, osteogenic differentiation was evaluated with an alkaline phosphatase bioassay and by analysis of gene expression by reverse transcription-polymerase chain reaction, and the extent of calcium deposition was measured using Alizarin Red S staining. Alendronate significantly increased cell numbers over control values, with the greatest effect at 10−8 M. Alkaline phosphatase activity and gene expression of bone morphogenetic protein 2, type I collagen and osteocalcin were increased after alendronate treatment. Alendronate also stimulated calcium deposition. We conclude that alendronate, apart from inhibiting osteoclastic bone resorption, is also a promoter of osteoblast proliferation and maturation.
The Efficacy of Serum Carcinoembryonic Antigen (CEA), Cancer Antigen 125 (CA125), Carbohydrate Antigen 19-9 (CA19-9), Carbohydrate Antigen 15-3 (CA15-3), α-Fetoprotein (AFP) and Human Chorionic Gonadotropin (hCG) Levels in Determining the Malignancy of Solitary Pulmonary Nodules
Authors: Bekci TT , Senol T , Maden E
Published: February 2013

We investigated the utility of the tumour markers carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), carbohydrate antigen 19-9 (CA19-9), carbohydrate antigen 15-3 (CA15-3), α-fetoprotein (AFP) and human chorionic gonadotropin (hCG) for the differential diagnosis of benign and malignant solitary pulmonary nodules in 42 hospitalized patients. Routine medical history and physical examination of each patient was performed and each patient also had a chest X-ray and a thoracic computed tomography scan. The following diagnostic procedures were also undertaken: bronchoscopy, transthoracic needle aspiration biopsy, sputum cytology and culture, analysis of sputum acid-fast bacilli and thoracotomy. Measurement of serum levels of tumour antigens by Immulite® 2000 radioimmunoassay found that three tumour markers, CEA, CA125 and CA15-3, could be used in the diagnosis of malignant solitary pulmonary nodules. More research is now required involving a larger group of patients.
Expression of CD34, α-Smooth Muscle Actin and Transforming Growth Factor-β1 in Squamous Intraepithelial Lesions and Squamous Cell Carcinoma of the Cervix
Authors: Li Q , Huang W , Zhou X
Published: February 2013

This retrospective study investigated CD34, α-smooth muscle actin (α-SMA), and transforming growth factor-β1 (TGF-β1) expression in stromal cells of cervical intraepithelial neoplasias (CINs; n = 30), invasive cervical squamous cell carcinomas (SCCs; n = 38) and adjacent normal cervix. Normal cervix and CINs contained diffuse CD34-positive stromal cells but no α-SMA-positive myofibroblasts. In contrast, 34 of 38 SCCs were free of CD34-positive stromal cells and all contained α-SMA-positive stromal myofibroblasts; adjacent normal tissue contained CD34-positive stromal cells and no α-SMA-positive myofibroblasts. More intense TGF-β1 expression was observed in SCC cells than in normal cervical epithelium or CINs. This study shows that the disappearance of CD34-positive stromal cells and appearance of α-SMA-positive stromal myofibroblasts may be associated with transformation of cervical CIN to SCC. These findings support the suggestion that over-production of TGF-β1 in SCC cells is one potential mechanism mediating the transformation of stromal cells to myofibroblasts in cervical carcinogenesis.
What Should be the Primary Treatment in Atrial Fibrillation: Ventricular Rate Control or Sinus Rhythm Control with Long-term Anticoagulation?
Authors: Okcun B , Yigit Z , Yildiz A , Uzunhasan I , Orta K , Baskurt M , Kaya A , Kucukoglu S
Published: February 2013

Recent trials have favoured ventricular rate control in atrial fibrillation (AF) management, however the present study investigated whether the restoration and maintenance of sinus rhythm with long-term anticoagulation therapy was superior in terms of embolic events and death in 534 patients with an AF duration > 48 h. Patients were randomized and received sinus rhythm control with either aspirin (group 1) or warfarin (group 2), or they were given ventricular rate control (group 3). Cardioversion to sinus rhythm was attempted in 425 patients and was successful in 387 (91.1%) of them. After 3 years' follow-up there were 12, two and 15 embolic events in groups 1, 2 and 3 respectively (significant difference between groups 1 and 2, and 2 and 3) and overall mortalities were four, two and 12, respectively (significant difference between groups 2 and 3). It is concluded that patients with an AF duration > 48 h might benefit considerably from sinus rhythm restoration and long-term warfarin therapy in terms of embolic events and mortality.
Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells
Authors: Huang S-Y , Zhang D-S , Han J-Q , Zhang N , Zhang S-Z , Mu W-L , Wei F-C
Published: February 2013

Herpes simplex virus thymidine kinase (HSV-TK) and Escherichia coli cytosine deaminase (CD) can convert innocuous prodrugs into cytotoxic metabolites and are being investigated for use in gene therapy for cancer. Human adenoid cystic carcinoma (ACC-2) cells transduced with a CD/HSV-TK fusion gene (ACC-2/CD-TK cells) were found to be more sensitive to radiation than ACC-2 cells when exposed to 5-fluorocytosine (5-FC; 40 μg/ml) plus ganciclovir (0.1 μg/ml) for 48 h before irradiation. Analysis of radiation survival curves for cells exposed to 5-FC plus ganciclovir before irradiation showed that ACC-2 cells had a higher capacity for sublethal damage repair (Dq value) and greater cellular radiosensitivity (D0 value) than ACC-2/CD-TK cells. Colony formation rate after 2 Gy of irradiation was significantly greater for ACC-2 than for ACC-2/CD-TK cells when cells were treated with 5-FC plus ganciclovir before irradiation. This study, therefore, indicates that addition of radiation might substantially improve the therapeutic potential of CD-TK fusion gene therapy of human adenoid cystic carcinomas.
Endotoxin Tolerance of RAW264.7 Correlates with p38-dependent Up-regulation of Scavenger Receptor-A
Authors: Xiang Q , Wen L , Liu MH , Zhang Y , Qu JF , Tian J
Published: February 2013

Pre-exposure to lipopolysaccharide (LPS) leads to hyposensitivity to secondary LPS stimulation, known as endotoxin tolerance. The role of macrophage scavenger receptor-A (SR-A) in endotoxin tolerance is unknown. In this study, LPS was shown to induce SR-A expression in the mouse macrophage cell line, RAW264.7, in dose- and time-dependent manners, which correlated with inflammatory cytokine suppression in RAW264.7 on secondary LPS stimulation. Over-expression of SR-A in RAW264.7 suppressed tumour necrosis factor (TNF)-α release and nuclear factor (NF)-kB activation, demonstrating the involvement of SR-A in endotoxin tolerance. LPS-pre-treated RAW264.7 cells could bind and internalize more fluorescein isothiocyanate (FITC)-LPS than untreated cells and both the SR-A ligand, fucoidan, and anti-SR-A 2F8 antibodies completely suppressed LPS-induced binding and internalization of FITC-LPS by RAW264.7. LPS-induced SR-A expression on RAW264.7 was completely suppressed by the p38-specific inhibitor, SB203580, but not by inhibition of toll-like receptor 4 (TLR4) signalling with MTS510, demonstrating that p38- but not TLR4-dependent up-regulation of SR-A was involved in endotoxin tolerance through binding and internalization of LPS.
Recombinant Human Erythropoietin Protects against Experimental Spinal Cord Trauma Injury by Regulating Expression of the Proteins MKP-1 and p-ERK
Authors: Huang H , Fan S , Ji X , Zhang Y , Bao F , Zhang G
Published: February 2013

The present study explored the tissue-protective effect of erythropoietin in rats after experimental spinal cord injury (SCI) produced by dropping a weight onto surgically exposed spinal cord. Sixty rats were randomized to sham operation (spinal cord exposure; control), SCI plus intraperitoneal saline injection, or SCI plus intraperitoneal erythropoietin injection. Locomotor function was evaluated with Basso, Beattie and Bresnahan scores 1 day (24 h) and 7 days later, and rats were then killed for analysis of lesion site tissue. Compared with saline-treated SCI rats, erythropoietin-treated SCI rats showed significantly less locomotor dysfunction and faster locomotor recovery. Immunohistochemistry showed that erythropoietin-treated SCI rats had a significantly lower phospho-extracellular signal-regulated kinase (p-ERK) protein expression and a significantly higher mitogen-activated protein kinase phosphatase-1 (MKP-1) protein expression than saline-treated SCI rats. Haematoxylin–eosin staining showed progressive disruption of dorsal white matter and neuron loss after SCI; lesions were less severe and there was more neuron regeneration in the erythropoietin group than in the saline group. It is concluded that erythropoietin reduces pathological changes and SCI severity via down-regulation of p-ERK and up-regulation of MKP-1.
The Neuroprotective and Anti-inflammatory Effects of Diltiazem in Spinal Cord Ischaemia–Reperfusion Injury
Authors: Fansa I , Altug ME , Melek I , Ucar E , Kontas T , Akcora B , Atik E , Duman T
Published: February 2013

The protective effects of diltiazem were examined in a rabbit model of spinal cord ischaemia–reperfusion induced by infrarenal aortic occlusion for 30 min. In the diltiazem group ( n = 6), an intravenous infusion (2 μg/kg per min) was started 10 min before ischaemia induction; normal saline solution was infused in the control group ( n = 6). Neurological function was assessed using modified Tarlov criteria 24 h after surgery. Plasma samples were analysed for interleukin (IL)-6 and IL-10. Spinal tissue was analysed for malondialdehyde, nitric oxide and reduced glutathione activities. Tarlov scores of the diltiazem-treated rabbits indicated significantly improved hind-limb motor function compared with the control group. The diltiazem group also had better quantitative and qualitative histopathological findings. Diltiazem infusion significantly reduced IL-6 levels 3 and 24 h after reperfusion compared with the control group. The mean IL-10 level in the diltiazem group was significantly higher than in the control group 24 h after reperfusion. It is concluded that diltiazem has cytoprotective and anti-inflammatory properties, leading to reduced spinal cord injury.
Exercise Training and Endothelial Progenitor Cells in Haemodialysis Patients
Authors: Manfredini F , Rigolin GM , Malagoni AM , Catizone L , Mandini S , Sofritti O , Mauro E , Soffritti S , Boari B , Cuneo A , Zamboni P , Manfredini R
Published: February 2013

Haemodialysis patients have few endothelial progenitor cells (EPCs) and an unfavourable cardiovascular outcome. The effects on peripheral blood CD34+ cells and EPCs of a 6-month walking exercise programme were studied. Thirty dialysis patients (20 males, age 67 ± 12 years) were prescribed exercise (two daily 10-min home walking sessions at moderate intensity, group E, n = 16) or not prescribed exercise (control, group C, n = 14). On entry and after 6 months peripheral blood CD34+ cells, EPCs (assessed as CD34+ cells co-expressing AC133 and vascular endothelial growth factor receptor 2 [VEGFR2], and as endothelial colony-forming units [e-CFU]) and exercise capacity (6-min walking distance, 6MWD) were evaluated. In group E, 6MWD and e-CFU increased significantly during the study period, with no significant changes in CD34+ or CD34+AC133+VEGFR2+ cell numbers. The change in e-CFU was directly and significantly correlated to patient-reported training load. Group C showed no significant change in any variable. In haemodialysis patients, moderate-intensity exercise selectively increased the number of e-CFU.
Evaluation of the Relationship between Anxiety and Depression and Bruxism
Authors: Gungormus Z , Erciyas K
Published: February 2013

This study examined the relationship between anxiety, depression and bruxism in 99 patients with temporomandibular disorders (TMD). Patients were divided into two groups: 58 patients with bruxism and 41 without bruxism. Symptoms of TMD were evaluated according to the Craniomandibular Index. The psychological condition of patients was assessed using the Beck Depression Inventory (BDI), the Hospital Anxiety Depression Scales (HADS) and the Hamilton Anxiety Rating Scale (HAMA). The mean anxiety and depression scores (HADS and HAMA) for patients with bruxism were statistically significant higher in patients with bruxism compared with those without bruxism. Thus, there may be an association between bruxism and higher levels of anxiety and/or depression in patients with TMD.
Quality of Life and the Effect on Social Status among Slovenian Women after Breast Cancer Treatment
Authors: Gorišek B , Krajnc P , Krajnc I
Published: February 2013

This study investigated whether the type of surgical procedure used to treat breast cancer (mastectomy versus breast-conserving lumpectomy) had any effect on the quality of life and social status of women. The prospective analysis included 382 women newly diagnosed with non-metastatic breast cancer who had undergone a surgical intervention for breast carcinoma at our institution: 198 patients had undergone mastectomy with axillary lymphadenectomy and 184 patients had undergone breast-conserving lumpectomy with local axillary lymphadenectomy. The post-mastectomy women reported significantly more financial problems, a lowered social status and more physical symptoms compared with the breast-conserving post-lumpectomy patients. The patients that underwent lumpectomy were more satisfied with their body image and their sexual life. Since the adverse financial effects of wage loss can significantly decrease a patient's quality of life, this study indicates that postmastectomy patients in particular need to be protected more effectively against a decline in their social status.
Effects of Losartan on Fibrinolytic Parameters and von Willebrand Factor in Chinese Subjects with Hypertension: A Comparative Study versus Atenolol
Authors: Liu J , Sun N-L , Yang J , Huang J-H
Published: February 2013

To compare the effects of losartan and atenolol on plasma fibrinolytic parameters and von Willebrand factor (vWF), Chinese subjects with mild-to-moderate hypertension were randomized to receive losartan (50 mg/day; n = 30) or atenolol (50 mg/day; n = 30) for 8 weeks. If target blood pressure (< 140/90 mmHg) was not achieved at week 4, hydrochlorothiazide (12.5 mg/day) was also administered. Plasma levels of tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1) and vWF were determined at baseline and after treatment. Between-group baseline characteristics and blood pressure decrease were comparable. Losartan significantly reduced plasma PAI-1 and vWF and PAI-1/tPA ratio. Atenolol significantly increased plasma tPA, but PAI-1, vWF and PAI-1/tPA ratio were unchanged. In conclusion, losartan, but not atenolol improved the fibrinolytic system and reduced plasma vWF levels in Chinese hypertensives.
A New Cell Culture System for Infection with Hepatitis B Virus that Fuses HepG2 Cells with Primary Human Hepatocytes
Authors: Jiang Y , Wang AH , Shao LH , Wang G , Yao YY , Sai LT , Chen FZ , Zheng F , Li Y , Ma LX
Published: February 2013

Hepatitis B virus (HBV) infection exhibits a very narrow host range and shows a strong tropism for liver parenchymal cells, however none of the previously established experimental models can reproduce the natural process of HBV infection. In the present study, primary human hepatocytes were fused with HepG2 cells to establish the hybrid HepCHLine-4 cell line with high susceptibility to HBV. The HepCHLine-4 cells expressed HBV-specific antigen when co-incubated with HBV-positive serum from a hepatitis B patient. Post-infection, HBV relaxed circular DNA and covalently closed circular DNA were detected in HepCHLine-4 cells using a nested polymerase chain reaction, and HBV-specific particles were visualized by electron microscopy of the culture media of HepCHLine-4 cells. HepG2 cells were not susceptible to HBV infection under the same conditions. The HepCHLine-4 cells can be sub-cultured for > 12 months while maintaining susceptibility to HBV and may, therefore, be useful for studying HBV infection and the viral life cycle in human hepatocytes.