bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Personality, Resilience and Life Conditions on Depression and Anxiety in 104 Patients Having Survived Acute Autoimmune Thrombotic Thrombocytopenic Purpura
Authors: Falter Tanja , Böschen Sibylle , Schepers Markus , Beutel Manfred , Lackner Karl , Scharrer Inge , Lämmle Bernhard
Published: January 2021

Autoimmune thrombotic thrombocytopenic purpura (iTTP) is a life-threatening, relapsing disease in which an acquired deficiency of the enzyme ADAMTS13 leads to generalised microvascular thrombosis. Survivors have a high prevalence of depression and impaired cognitive function. The aim of this study was to determine whether life circumstances and personality have an influence on the development and severity of depression and anxiety in iTTP patients and how they impact the quality of life. With validated questionnaires, we examined the prevalence of depression and anxiety symptoms in 104 iTTP patients, as well as parameters of subjective cognitive deficits, quality of life, attitude to life and resilience. iTTP patients had significantly more depressive symptoms (p < 0.001), a tendency to have anxiety disorders (p = 0.035) and a significantly worse cognitive performance (p = 0.008) compared to the controls. Sex, age, physical activity and partnership status had no significant influence on depression, whereas the number of comorbidities did. Lower scores of resilience, attitude to life and quality of life were reported by patients compared to controls. iTTP patients had a high prevalence of depression and anxiety, as well as a more negative attitude to life and low resilience. Resilience correlated negatively with the severity of the depression. Furthermore, quality of life and cognitive performance were significantly reduced.
Structural Analysis of a Barge Midship Section Considering the Still Water and Wave Load Effects
Authors: Salazar-Domínguez Cristian M. , Hernández-Hernández José , Rosas-Huerta Edna D. , Iturbe-Rosas Gustavo E. , Herrera-May Agustín L.
Published: January 2021

Structural failures in the barge midship sections can cause operational delay, sinking, cargo loss and environmental damage. These failures can be generated by the barge and cargo weights, and wave load effects on the midships sections. These load types must be considered in the design of the barge midship sections. Here, we present the structural analysis of a barge midship section that has decreased up to 36.4% of its deck thickness caused by corrosion. This analysis is developed using finite element method (FEM) models that include the barge and cargo weights, and wave load effects. The FEM models regarded three cargo tanks in the midship section, containing the main longitudinal and transverse structural elements. In addition, the hull girder section modulus and the required deck thickness of the barge were calculated using Lloyd’s Register rules. These rules were applied to estimate the permissible bending stresses at deck and bottom plates under sagging and hogging conditions, which agreed well with those of the FEM models. Based on FEM models, the maximum compressive normal stress and von Mises stress of the hull girder structure were 175.54 MPa and 215.53 MPa, respectively. These stress values do not overcome the yield strength (250 MPa) of the barge material, allowing a safe structural behavior of the barge. The structural modeling of the barge midship section can predict its structural behavior under different sagging and hogging conditions, considering the cargo, weight and wave loads.
Rapid Antifungal Susceptibility Testing of Yeasts and Molds by MALDI-TOF MS: A Systematic Review and Meta-Analysis
Authors: Knoll Miriam Alisa , Ulmer Hanno , Lass-Flörl Cornelia
Published: January 2021

Due to the growing burden of fungal infections and a recent rise in antifungal resistance, antifungal susceptibility testing (AFST) is of increasing importance. The common methods of AFST have turnaround times of 24 to 48 h, and the available rapid methods are limited by applicability, cost-efficiency or accuracy. Given the urgency of adequate antifungal treatment in invasive mycoses, the need for the rapid and reliable detection of resistance is evident. In this systematic review and meta-analysis, we evaluated the diagnostic accuracy of AFST based on matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Twelve studies were reviewed, and data for the comparative analysis of their accuracy and methodology were systematically extracted. Compared to broth dilution as the gold standard, MALDI-TOF MS-based AFST reached a pooled sensitivity and specificity of 91% (95% Confidence Interval [CI], 84% to 96%) and 95% (95% CI, 90% to 98%), respectively. A comparative analysis showed that the sensitivity was higher for the semi-quantitative matrix-assisted laser desorption ionization Biotyper antibiotic susceptibility test rapid assay (MBT ASTRA) technique (96%) than for the correlate composite index (CCI) approach (85%), which is based on spectrum changes. Turnaround times below eight hours reached better diagnostic values than longer incubation periods, qualifying MALDI-TOF MS-based AFST as a rapid and accurate method for the detection of antifungal resistance.
Age by Single Nucleotide Polymorphism Interactions on Bronchodilator Response in Asthmatics
Authors: Voorhies Kirsten , Sordillo Joanne E. , McGeachie Michael , Ampleford Elizabeth , Wang Alberta L. , Lasky-Su Jessica , Tantisira Kelan , Dahlin Amber , Kelly Rachel S. , Ortega Victor E. , Lutz Sharon M. , Wu Ann C.
Published: January 2021

An unaddressed and important issue is the role age plays in modulating response to short acting β2-agonists in individuals with asthma. The objective of this study was to identify whether age modifies genetic associations of single nucleotide polymorphisms (SNPs) with bronchodilator response (BDR) to β2-agonists. Using three cohorts with a total of 892 subjects, we ran a genome wide interaction study (GWIS) for each cohort to examine SNP by age interactions with BDR. A fixed effect meta-analysis was used to combine the results. In order to determine if previously identified BDR SNPs had an age interaction, we also examined 16 polymorphisms in candidate genes from two published genome wide association studies (GWAS) of BDR. There were no significant SNP by age interactions on BDR using the genome wide significance level of 5 × 10−8. Using a suggestive significance level of 5 × 10−6, three interactions, including one for a SNP within PRAG1 (rs4840337), were significant and replicated at the significance level of 0.05. Considering candidate genes from two previous GWAS of BDR, three SNPs (rs10476900 (near ADRB2) [p-value = 0.009], rs10827492 (CREM) [p-value = 0.02], and rs72646209 (NCOA3) [p-value = 0.02]) had a marginally significant interaction with age on BDR (p < 0.05). Our results suggest age may be an important modifier of genetic associations for BDR in asthma.
Impact of Particle Size on Performance of Selective Laser Sintering Walnut Shell/Co-PES Powder
Authors: Yu Yueqiang , Jiang Minzheng , Wang Suling , Guo Yanling , Jiang Ting , Zeng Weiliang , Zhuang Yu
Published: January 2021

The agricultural and forestry waste walnut shell and copolyester hot-melt adhesives (Co-PES) powder were selected as feedstock. A kind of low-cost, low-power consumption, and environmentally friendly walnut shell/Co-PES powder composites (WSPC) was used for selective laser sintering (SLS). Though analyzing the size and morphology of walnut shell particle (≤550 μm) as well as performing an analysis of surface roughness, density, and mechanical test of WSPC parts with different particle sizes, results showed that the optimal mechanical performance (tensile strength of 2.011 MPa, bending strength of 3.5 MPa, impact strength of 0.718 KJ/m2) as walnut shell powder particle size was 80 to 120 μm. When walnut shell powder particle diameter was 120 to 180 μm, the minimum value of surface roughness of WSPC parts was 15.711 μm and density was approximately the maximum (0.926 g/cm3).
Acousto-Optic Cells with Phased-Array Transducers and Their Application in Systems of Optical Information Processing
Authors: Balakshy Vladimir , Kupreychik Maxim , Mantsevich Sergey , Molchanov Vladimir
Published: January 2021

This paper presents the results of theoretical and experimental studies of anisotropic acousto-optic interaction in a spatially periodical acoustic field created by a phased-array transducer with antiphase excitation of adjacent sections. In this case, contrary to the nonsectioned transducer, light diffraction is absent when the optical beam falls on the phased-array cell at the Bragg angle. However, the diffraction takes place at some other angles (called “optimal” here), which are situated on the opposite sides to the Bragg angle. Our calculations show that the diffraction efficiency can reach 100% at these optimal angles in spite of a noticeable acousto-optic phase mismatch. This kind of acousto-optic interaction possesses a number of interesting regularities which can be useful for designing acousto-optic devices of a new type. Our experiments were performed with a paratellurite (TeO2) cell in which a shear acoustic mode was excited at a 9∘ angle to the crystal plane (001). The piezoelectric transducer had to nine antiphase sections. The efficiency of electric to acoustic power conversion was 99% at the maximum frequency response, and the ultrasound excitation band extended from 70 to 160 MHz. The experiments have confirmed basic results of the theoretical analysis.
Gravity-Driven Separation of Oil/Water Mixture by Porous Ceramic Membranes with Desired Surface Wettability
Authors: Ren Chunlei , Chen Wufeng , Chen Chusheng , Winnubst Louis , Yan Lifeng
Published: January 2021

Porous Al2O3 membranes were prepared through a phase-inversion tape casting/sintering method. The alumina membranes were embedded with finger-like pores perpendicular to the membrane surface. Bare alumina membranes are naturally hydrophilic and underwater oleophobic, while fluoroalkylsilane (FAS)-grafted membranes are hydrophobic and oleophilic. The coupling of FAS molecules on alumina surfaces was confirmed by Thermogravimetric Analysis and X-ray Photoelectron Spectroscopy measurements. The hydrophobic membranes exhibited desired thermal stability and were super durable when exposed to air. Both membranes can be used for gravity-driven oil/water separation, which is highly cost-effective. The as-calculated separation efficiency (R) was above 99% for the FAS-grafted alumina membrane. Due to the excellent oil/water separation performance and good chemical stability, the porous ceramic membranes display potential for practical applications.
Structure of Plasma (re)Polymerized Polylactic Acid Films Fabricated by Plasma-Assisted Vapour Thermal Deposition
Authors: Krtouš Zdeněk , Hanyková Lenka , Krakovský Ivan , Nikitin Daniil , Pleskunov Pavel , Kylián Ondřej , Sedlaříková Jana , Kousal Jaroslav
Published: January 2021

Plasma polymer films typically consist of very short fragments of the precursor molecules. That rather limits the applicability of most plasma polymerisation/plasma-enhanced chemical vapour deposition (PECVD) processes in cases where retention of longer molecular structures is desirable. Plasma-assisted vapour thermal deposition (PAVTD) circumvents this limitation by using a classical bulk polymer as a high molecular weight “precursor”. As a model polymer in this study, polylactic acid (PLA) has been used. The resulting PLA-like films were characterised mostly by X-ray photoelectron spectroscopy (XPS) and nuclear magnetic resonance (NMR) spectroscopy. The molecular structure of the films was found to be tunable in a broad range: from the structures very similar to bulk PLA polymer to structures that are more typical for films prepared using PECVD. In all cases, PLA-like groups are at least partially preserved. A simplified model of the PAVTD process chemistry was proposed and found to describe well the observed composition of the films. The structure of the PLA-like films demonstrates the ability of plasma-assisted vapour thermal deposition to bridge the typical gap between the classical and plasma polymers.
Ecotoxicity and Essential Properties of Fine-Recycled Aggregate
Authors: Mariaková Diana , Anna Mocová Klára , Fořtová Kristina , Ryparová Pavla , Pešta Jan , Pavlů Tereza
Published: January 2021

This article deals with the possibility of utilization of secondary-raw materials as a natural sand replacement in concrete. Four types of waste construction materials were examined—recycled aggregate from four different sources. The natural aggregate was examined as well as used as the reference sample. All the samples were tested to evaluate the water absorption, particle size distribution, and particle density. The basic chemical reactions in the view of ecotoxicology are investigated and measured based on Czech standards. Chemical analysis, Lemna growth inhibition test, freshwater algae, daphnia acute, and mustard germination toxicity test were made and discussed in this paper. Based on the physical and geometrical properties and ecotoxicology of examined waste materials, this work evaluated them as suitable for utilization in concrete as a sand replacement.
Estimating a Stoichiometric Solid’s Gibbs Free Energy Model by Means of a Constrained Evolutionary Strategy
Authors: Grau Turuelo Constantino , Pinnau Sebastian , Breitkopf Cornelia
Published: January 2021

Modeling of thermodynamic properties, like heat capacities for stoichiometric solids, includes the treatment of different sources of data which may be inconsistent and diverse. In this work, an approach based on the covariance matrix adaptation evolution strategy (CMA-ES) is proposed and described as an alternative method for data treatment and fitting with the support of data source dependent weight factors and physical constraints. This is applied to a Gibb’s Free Energy stoichiometric model for different magnesium sulfate hydrates by means of the NASA9 polynomial. Its behavior is proved by: (i) The comparison of the model to other standard methods for different heat capacity data, yielding a more plausible curve at high temperature ranges; (ii) the comparison of the fitted heat capacity values of MgSO4·7H2O against DSC measurements, resulting in a mean relative error of a 0.7% and a normalized root mean square deviation of 1.1%; and (iii) comparing the Van’t Hoff and proposed Stoichiometric model vapor-solid equilibrium curves to different literature data for MgSO4·7H2O, MgSO4·6H2O, and MgSO4·1H2O, resulting in similar equilibrium values, especially for MgSO4·7H2O and MgSO4·6H2O. The results show good agreement with the employed data and confirm this method as a viable alternative for fitting complex physically constrained data sets, while being a potential approach for automatic data fitting of substance data.
Voltage Profile Enhancement and Loss Minimization Using Optimal Placement and Sizing of Distributed Generation in Reconfigured Network
Authors: Haider Waseem , Hassan S Jarjees Ul , Mehdi Arif , Hussain Arif , Adjayeng Gerardo Ondo Micha , Kim Chul-Hwan
Published: January 2021

Power loss and voltage instability are major problems in distribution systems. However, these problems are typically mitigated by efficient network reconfiguration, including the integration of distributed generation (DG) units in the distribution network. In this regard, the optimal placement and sizing of DGs are crucial. Otherwise, the network performance will be degraded. This study is conducted to optimally locate and sizing of DGs into a radial distribution network before and after reconfiguration. A multi-objective particle swarm optimization algorithm is utilized to determine the optimal placement and sizing of the DGs before and after reconfiguration of the radial network. An optimal network configuration with DG coordination in an active distribution network overcomes power losses, uplifts voltage profiles, and improves the system stability, reliability, and efficiency. For considering the actual power system scenarios, a penalty factor is also considered, this penalty factor plays a crucial role in the minimization of total power loss and voltage profile enhancement. The simulation results showed a significant improvement in the percentage power loss reduction (32% and 68.05% before and after reconfiguration, respectively) with the inclusion of DG units in the test system. Similarly, the minimum bus voltage of the system is improved by 4.9% and 6.53% before and after reconfiguration, respectively. The comparative study is performed, and the results showed the effectiveness of the proposed method in reducing the voltage deviation and power loss of the distribution system. The proposed algorithm is evaluated on the IEEE-33 bus radial distribution system, using MATLAB software.
Dynamics of a Pair of Paramagnetic Janus Particles under a Uniform Magnetic Field and Simple Shear Flow
Authors: Sobecki Christopher , Zhang Jie , Wang Cheng
Published: January 2021

We numerically investigate the dynamics of a pair of circular Janus microparticles immersed in a Newtonian fluid under a simple shear flow and a uniform magnetic field by direct numerical simulation. Using the COMSOL software, we applied the finite element method, based on an arbitrary Lagrangian-Eulerian approach, and analyzed the dynamics of two anisotropic particles (i.e., one-half is paramagnetic, and the other is non-magnetic) due to the center-to-center distance, magnetic field strength, initial particle orientation, and configuration. This article considers two configurations: the LR-configuration (magnetic material is on the left side of the first particle and on the right side of the second particle) and the RL-configuration (magnetic material is on the right side of the first particle and on the left side of the second particle). For both configurations, a critical orientation determines if the particles either attract (below the critical) or repel (above the critical) under a uniform magnetic field. How well the particles form a chain depends on the comparison between the viscous and magnetic forces. For long particle distances, the viscous force separates the particles, and the magnetic force causes them to repel as the particle orientation increases above the configuration’s critical value. As the initial distance decreases, a chain formation is possible at a steady orientation, but is more feasible for the RL-configuration than the LR-configuration under the same circumstances.
Modeling Recoveries of US Leading Banks Based on Publicly Disclosed Data
Authors: Siarka Pawel
Published: January 2021

The credit risk management process is a critical element that allows financial institutions to withstand economic downturns. Unlike the methods regarding the probability of default, which have been deeply addressed after the financial crisis in 2008, recovery rate models still need further development. As there are no industry standards, leading banks are modeling recovery rates using internal models developed with different assumptions. Therefore, the outcomes are often incomparable and may lead to confusion. The author presents the concept of a unified recovery rate analysis for US banks. He uses data derived from FR Y-9C reports disclosed by the Federal Reserve Bank of Chicago. Based on the historical recoveries and credit portfolio book values, the author examines the distribution function of recoveries. The research refers to a credit card portfolio and covers nine leading US banks. The author leveraged Vasicek’s one-factor model with the asset correlation parameter and implemented it for recovery rate analysis. This experiment revealed that the estimated latent correlation ranges from 0.2% to 1.5% within the examined portfolios. They are large enough to impact the recovery rate volatility and cannot be treated as negligible. It was shown that the presented method could be applied under US Comprehensive Capital Analysis and Review exercise.
IgE-Mediated Fish Allergy in Children
Authors: Buyuktiryaki Betul , Masini Marzio , Mori Francesca , Barni Simona , Liccioli Giulia , Sarti Lucrezia , Lodi Lorenzo , Giovannini Mattia , du Toit George , Lopata Andreas Ludwig , Marques-Mejias Maria Andreina
Published: January 2021

Fish allergy constitutes a severe problem worldwide. Its prevalence has been calculated as high as 7% in paediatric populations, and in many cases, it persists into adulthood with life-threatening signs and symptoms. The following review focuses on the epidemiology of Immunoglobulin E (IgE)-mediated fish allergy, its pathogenesis, clinical manifestations, and a thorough approach to diagnosis and management in the paediatric population. The traditional approach for managing fish allergy is avoidance and rescue medication for accidental exposures. Food avoidance poses many obstacles and is not easily maintained. In the specific case of fish, food is also not the only source of allergens; aerosolisation of fish proteins when cooking is a common source of highly allergenic parvalbumin, and elimination diets cannot prevent these contacts. Novel management approaches based on immunomodulation are a promising strategy for the future of these patients.
Laparoscopic versus Open Transverse-Incision Approach for Right Hemicolectomy: A Systematic Review and Meta-Analysis
Authors: Feo Claudio F. , Paliogiannis Panagiotis , Fancellu Alessandro , Zinellu Angelo , Ginesu Giorgio C. , Feo Carlo V. , Porcu Alberto
Published: January 2021

Background and Objectives: There is general agreement on the benefits of laparoscopy for treatment of rectal and left colon cancers, whereas findings regarding the comparison of laparoscopic and open right colonic resections are discordant. The aim of this systematic review and meta-analysis was to assess the outcomes and advantages of laparoscopic versus transverse-incision open surgery for management of right colon cancer. Materials and Methods: A systematic review was performed according to the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines. Comparative studies evaluating the results of laparoscopic and transverse-incision open right hemicolectomies were analyzed. The measured outcomes were mean operative time, time to feeding, duration of hospital stay, and number of lymph nodes harvested. Results: A total of 5 studies including 318 patients met the inclusion criteria. Meta-analysis revealed no differences in time to resume oral feeding, hospital stay, and number of lymph nodes harvested in between groups, but mean length of surgery was significantly longer in the laparoscopic group. Conclusion: These data confirm that the preferred approach to right hemicolectomy is yet unclear. Laparoscopy has a longer operative time than transverse-incision open surgery, and no significant short-term benefits were observed for the studied parameters. Well-designed randomized control trials (RCTs) might help to identify the differences between these two techniques for the surgical treatment of right colon cancer.
Commercial Pressure Retarded Osmosis Systems for Seawater Desalination Plants
Authors: Makabe Ryo , Ueyama Tetsuro , Sakai Hideyuki , Tanioka Akihiko
Published: January 2021

The development of renewable energy technologies is of global importance. To realize a sustainable society, fossil-resource-independent technologies, such as solar- and wind-power generation, should be widely adopted. Pressure retarded osmosis (PRO) is one such potential renewable energy technology. PRO requires salt water and fresh water, both of which can be found at seawater desalination plants. The total power generation capacity of PRO, using concentrated seawater and fresh water, is 3 GW. A large amount of energy is required for seawater desalination; therefore, the introduction of renewable energy should be prioritized. Kyowakiden Industry Co., Ltd., has been working on introducing PRO to seawater desalination plants since 2001 and is attracting attention for its ongoing PRO pilot plant with a scale of 460 m3/d, using concentrated seawater and treated sewage water. In this study, we evaluated the feasibility of introducing PRO in existing desalination plants. The feasibility was examined based on technology, operation, and economy. Based on the number of seawater desalination plants in each country and the electricity charges, it was determined whether the introduction of PRO would be viable.
Compression Behavior of Sheets Metals of Pure Titanium 2 and Ti6Al4V Alloy under High Temperature: Evaluation of the Tension–Compression Asymmetry
Authors: Ayllón Jorge , Miguel Valentín , Martínez-Martínez Alberto , Coello Juana , Naranjo Jesús Andrés , García-Sevilla Francisco
Published: January 2021

Determining the intrinsic indices of sheet metals under compression states at high temperatures is vital to accurately predict the behavior of the material in warm/hot forming processes. Nevertheless, the literature contains little previous experimental data in this regard due to the difficulty of carrying out specific test methodologies in sheet metals. The authors of the present manuscript previously developed an approach to evaluate the in-plane compression behavior under a wide range of test conditions, which was applied here to characterize pure titanium and Ti6Al4V alloy until 750 °C. This procedure allowed us to quantify the asymmetric and anisotropic tension–compression (T-C) response of the materials involved and their evolution with temperature and strain rate. The asymmetry detected at room temperature showed a higher compression response in all cases, mostly reaching differences of around 10%. For the lowest strain rate studied, the typical assumed symmetric T-C behavior was observed from 300 and 450 °C onwards, for the rolling and transverse direction, respectively. In addition, stepped compression tests led us to deduce the anisotropy indices, which were different from those found under tension, in contrast to the r-values applied by most authors. Using the experimental results, a factor related to the asymmetry found was proposed to formulate an extended constitutive model. The asymmetry and anisotropy data supplied for compression under warm/hot conditions are the main novelty of this research.
Physical Activity-Related Metabolites Are Associated with Mortality: Findings from the Atherosclerosis Risk in Communities (ARIC) Study
Authors: Xu Jun , Liu Guning , Hegde Sheila M. , Palta Priya , Boerwinkle Eric , Gabriel Kelley P. , Yu Bing
Published: January 2021

Habitual physical activity can diminish the risk of premature death. Identifying a pattern of metabolites related to physical activity may advance our understanding of disease etiology. We quantified 245 serum metabolites in 3802 participants from the Atherosclerosis Risk in Communities (ARIC) study using chromatography–mass spectrometry. We regressed self-reported moderate-to-vigorous intensity leisure-time physical activity (LTPA) against each metabolite, adjusting for traditional risk factors. A standardized metabolite risk score (MRS) was constructed to examine its association with all-cause mortality using the Cox proportional hazard model. We identified 10 metabolites associated with LTPA (p < 2.04 × 10−4) and established that an increase of one unit of the metabolic equivalent of task-hours per week (MET·hr·wk−1) in LTPA was associated with a 0.012 SD increase in MRS. During a median of 27.5 years of follow-up, we observed 1928 deaths. One SD increase of MRS was associated with a 10% lower risk of death (HR = 0.90, 95% CI: 0.85–0.95). The highest vs. the lowest MRS quintile rank was associated with a 22% reduced risk of death (HR = 0.78, 95% CI: 0.62–0.94). The effects were consistent across race and sex groups. In summary, we identified a set of metabolites associated with LTPA and an MRS associated with a lower risk of death. Our study provides novel insights into the potential mechanisms underlying the health impacts of physical activity.
Quantification of All-Trans Retinoic Acid by Liquid Chromatography–Tandem Mass Spectrometry and Association with Lipid Profile in Patients with Type 2 Diabetes
Authors: Morgenstern Jakob , Fleming Thomas , Kliemank Elisabeth , Brune Maik , Nawroth Peter , Fischer Andreas
Published: January 2021

Retinoic acids are vitamin A metabolites that have numerous essential functions in humans, and are also used as drugs to treat acne and acute promyelocytic leukemia. All-trans retinoic acid (atRA) is the major occurring metabolite of retinoic acid in humans. This study provides a sensitive and specific liquid chromatography–tandem mass spectrometry approach in order to quantify atRA in human plasma samples. The isolation of atRA by hyperacidified liquid–liquid extraction using hexane and ethyl acetate resulted in a recovery of 89.7 ± 9.2%. The lower limit of detection was 20 pg·mL−1, and 7 point calibration displayed good linearity (R2 = 0.994) in the range of 50–3200 pg mL−1. Selectivity was guaranteed by the use of two individual mass transitions (qualifier and quantifier), and precision and accuracy were determined intraday and interday with a coefficient variation of 9.3% (intraday) and 14.0% (interday). Moreover, the method could be used to isolate atRA from hyperlipidemic samples. Applying this method to plasma samples from patients with poorly controlled Type 2 diabetes significantly decreased atRA plasma levels as compared to those of the healthy controls. In addition, atRA concentrations were highly associated with increased low-density lipoprotein (LDL) and decreased high-density lipoprotein (HDL) cholesterol levels.
3D-Printed Microfluidic Droplet Generator with Hydrophilic and Hydrophobic Polymers
Authors: Warr Chandler A. , Hinnen Hunter S. , Avery Saroya , Cate Rebecca J. , Nordin Gregory P. , Pitt William G.
Published: January 2021

Droplet generation has been widely used in conventional two-dimensional (2D) microfluidic devices, and has recently begun to be explored for 3D-printed droplet generators. A major challenge for 3D-printed devices is preventing water-in-oil droplets from sticking to the interior surfaces of the droplet generator when the device is not made from hydrophobic materials. In this study, two approaches were investigated and shown to successfully form droplets in 3D-printed microfluidic devices. First, several printing resin candidates were tested to evaluate their suitability for droplet formation and material properties. We determined that a hexanediol diacrylate/lauryl acrylate (HDDA/LA) resin forms a solid polymer that is sufficiently hydrophobic to prevent aqueous droplets (in a continuous oil flow) from attaching to the device walls. The second approach uses a fully 3D annular channel-in-channel geometry to form microfluidic droplets that do not contact channel walls, and thus, this geometry can be used with hydrophilic resins. Stable droplets were shown to form using the channel-in-channel geometry, and the droplet size and generation frequency for this geometry were explored for various flow rates for the continuous and dispersed phases.
Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon
Authors: Khaled Salma , Gantois Nausicaa , Ayoubi Aisha , Even Gaël , Sawant Manasi , El Houmayraa Jinane , Nabot Mathieu , Benamrouz-Vanneste Sadia , Chabé Magali , Certad Gabriela , El Safadi Dima , Dabboussi Fouad , Hamze Monzer , Viscogliosi Eric
Published: January 2021

Molecular data concerning the prevalence and subtype (ST) distribution of the intestinal parasite Blastocystis sp. remain scarce in the Middle East. Accordingly, we performed the first molecular epidemiological survey ever conducted in the Syrian population. A total of 306 stool samples were collected from Syrian refugees living in 26 informal tented settlements (ITS) subjected or not to water, sanitation, and hygiene (WASH) interventions in North Lebanon, then screened for the presence of Blastocystis sp. by real-time polymerase chain reaction followed by subtyping. The overall prevalence of the parasite was shown to reach 63.7%. Blastocystis sp. colonization was not significantly associated with gender, age, symptomatic status, abdominal pain or diarrhea. In contrast, WASH intervention status of ITS was identified as a risk factor for infection. Among a total of 164 subtyped isolates, ST3 was predominant, followed by ST1, ST2, and ST10. No particular ST was reported to be associated with age, gender, symptomatic status, digestive disorders, or WASH intervention status of ITS. Intra-ST diversity of ST1 to ST3 was low suggesting large-scale anthroponotic transmission. Moreover, comparative analysis of ST1 to ST3 genotypes revealed that the circulation of the parasite between Syrian refugees and the host population was likely limited.
The HrpG/HrpX Regulon of Xanthomonads—An Insight to the Complexity of Regulation of Virulence Traits in Phytopathogenic Bacteria
Authors: Teper Doron , Pandey Sheo Shankar , Wang Nian
Published: January 2021

Bacteria of the genus Xanthomonas cause a wide variety of economically important diseases in most crops. The virulence of the majority of Xanthomonas spp. is dependent on secretion and translocation of effectors by the type 3 secretion system (T3SS) that is controlled by two master transcriptional regulators HrpG and HrpX. Since their discovery in the 1990s, the two regulators were the focal point of many studies aiming to decipher the regulatory network that controls pathogenicity in Xanthomonas bacteria. HrpG controls the expression of HrpX, which subsequently controls the expression of T3SS apparatus genes and effectors. The HrpG/HrpX regulon is activated in planta and subjected to tight metabolic and genetic regulation. In this review, we cover the advances made in understanding the regulatory networks that control and are controlled by the HrpG/HrpX regulon and their conservation between different Xanthomonas spp.
Metagenomics Approaches for the Detection and Surveillance of Emerging and Recurrent Plant Pathogens
Authors: Piombo Edoardo , Abdelfattah Ahmed , Droby Samir , Wisniewski Michael , Spadaro Davide , Schena Leonardo
Published: January 2021

Globalization has a dramatic effect on the trade and movement of seeds, fruits and vegetables, with a corresponding increase in economic losses caused by the introduction of transboundary plant pathogens. Current diagnostic techniques provide a useful and precise tool to enact surveillance protocols regarding specific organisms, but this approach is strictly targeted, while metabarcoding and shotgun metagenomics could be used to simultaneously detect all known pathogens and potentially new ones. This review aims to present the current status of high-throughput sequencing (HTS) diagnostics of fungal and bacterial plant pathogens, discuss the challenges that need to be addressed, and provide direction for the development of methods for the detection of a restricted number of related taxa (specific surveillance) or all of the microorganisms present in a sample (general surveillance). HTS techniques, particularly metabarcoding, could be useful for the surveillance of soilborne, seedborne and airborne pathogens, as well as for identifying new pathogens and determining the origin of outbreaks. Metabarcoding and shotgun metagenomics still suffer from low precision, but this issue can be limited by carefully choosing primers and bioinformatic algorithms. Advances in bioinformatics will greatly accelerate the use of metagenomics to address critical aspects related to the detection and surveillance of plant pathogens in plant material and foodstuffs.
Genomic Features of MCR-1 and Extended-Spectrum β-Lactamase-Producing Enterobacterales from Retail Raw Chicken in Egypt
Authors: Sadek Mustafa , Ortiz de la Rosa José Manuel , Abdelfattah Maky Mohamed , Korashe Dandrawy Mohamed , Nordmann Patrice , Poirel Laurent
Published: January 2021

Colistin is considered as a last resort agent for treatment of severe infections caused by carbapenem-resistant Enterobacterales (CRE). Recently, plasmid-mediated colistin resistance genes (mcr type) have been reported, mainly corresponding to mcr-1 producers. Those mcr-1-positive Enterobacterales have been identified not only from human isolates, but also from food samples, from animal specimens and from environmental samples in various parts of the world. Our study focused on the occurrence and characterization of mcr-1-positive Enterobacterales recovered from retail raw chicken in Egypt. From the 345 retail chicken carcasses collected, a total of 20 samples allowed to recover mcr-1-positive isolates (Escherichia coli, n = 19; Citrobacter freundii, n = 1). No mcr-2- to mcr-10-positive isolate was identified from those samples. The colistin resistance trait was confirmed for all those 20 isolates with a positivity of the Rapid Polymyxin NP (Nordmann-Poirel) test. Minimum inhibitory concentrations (MICs) of colistin for all MCR-1-producing isolates ranged between 4 and 16 μg/mL. Noticeably, 9 out of the 20 mcr-1-positive isolates produced an extended-spectrum β-lactamase (ESBL), respectively producing CTX-M-9 (n = 2), CTX-M-14 (n = 4), CTX-M-15 (n = 2), and SHV-12 (n = 1). Noteworthy, the fosA4 gene encoding resistance to fosfomycin was found in a single mcr-1-positive E. coli isolate, in which both genes were located on different conjugative plasmids. The pulsed-field gel electrophoresis (PFGE) patterns were identified, corresponding to 10 different sequence types (STs), highlighting the genetic diversity of those different E. coli. Whole-genome sequencing revealed three major types of mcr-1-bearing plasmids, corresponding to IncI2, IncX4, and IncHI2 scaffolds. The occurrence of MCR-1-producing multidrug-resistant Enterobacterales in retail raw chicken is of great concern, considering the possibility of transmission to humans through the food chain.
Corynebacterium glutamicum Mechanosensing: From Osmoregulation to L-Glutamate Secretion for the Avian Microbiota-Gut-Brain Axis
Authors: Nakayama Yoshitaka
Published: January 2021

After the discovery of Corynebacterium glutamicum from avian feces-contaminated soil, its enigmatic L-glutamate secretion by corynebacterial MscCG-type mechanosensitive channels has been utilized for industrial monosodium glutamate production. Bacterial mechanosensitive channels are activated directly by increased membrane tension upon hypoosmotic downshock; thus; the physiological significance of the corynebacterial L-glutamate secretion has been considered as adjusting turgor pressure by releasing cytoplasmic solutes. In this review, we present information that corynebacterial mechanosensitive channels have been evolutionally specialized as carriers to secrete L-glutamate into the surrounding environment in their habitats rather than osmotic safety valves. The lipid modulation activation of MscCG channels in L-glutamate production can be explained by the “Force-From-Lipids” and “Force-From-Tethers” mechanosensing paradigms and differs significantly from mechanical activation upon hypoosmotic shock. The review also provides information on the search for evidence that C. glutamicum was originally a gut bacterium in the avian host with the aim of understanding the physiological roles of corynebacterial mechanosensing. C. glutamicum is able to secrete L-glutamate by mechanosensitive channels in the gut microbiota and help the host brain function via the microbiota–gut–brain axis.
Study on the Strength Evolution Characteristics of Cemented Tailings Backfill from the Perspective of Porosity
Authors: Deng Hongwei , Liu Yao , Zhang Weiyou , Yu Songtao , Tian Guanglin
Published: January 2021

At present, the filling mining method is widely used. To study strength evolution laws of cemented tailings backfill (CTB) under different curing ages, in the experiment, mine tailings were used as aggregates, ordinary Portland cement (PC32.5) was used as cementing materials, and different additives (lime and fly ash) were added to make filling samples with the solids mass concentration at 74% and the cement-sand ratios 1:4, 1:6 and 1:8. Based on the nuclear magnetic resonance (NMR) technology, the porosity test of filling samples with curing ages of 3 d, 7 d and 28 d was carried out, and the uniaxial compressive strength test was carried out on the servo universal material testing machine. The relationship between the uniaxial compressive strength and porosity of backfills and the curing age in the three groups was studied, and change laws of the porosity variation and strength growth rate of backfills were analyzed. Based on the variation in porosity, the strength evolution model of the CTB under different curing ages was established, and the model was fitted and verified with test data. Results show that the uniaxial compressive strength, porosity, porosity variation, and strength growth rate of the three groups of backfills gradually increase with the increase of the curing age, the porosity of backfill basically increases with the decrease of the cement–sand ratio, and the porosity of backfill decreases with the increase of the curing age. Porosity variations and relative strength values of the three groups of backfills under different cement-sand ratios obey an exponential function, and the two have a good correlation, indicating that the established filling strength evolution model can well reflect strength evolution laws of the CTB with the change of curing age.
Fly Ash Utilisation in Mullite Fabrication: Development of Novel Percolated Mullite
Authors: Koshy Pramod , Ho Naomi , Zhong Vicki , Schreck Luisa , Koszo Sandor Alex , Severin Erik J. , Sorrell Charles Christopher
Published: January 2021

Fly ash is an aluminosilicate and the major by-product from coal combustion in power stations; its increasing volumes are major economic and environmental concerns, particularly since it is one of the largest mineral resources based on current estimates. Mullite (3Al2O3·2SiO2) is the only stable phase in the Al2O3-SiO2 system and is used in numerous applications owing to its high-temperature chemical and mechanical stabilities. Hence, fly ash offers a potential economical resource for mullite fabrication, which is confirmed by a review of the current literature. This review details the methodologies to utilise fly ash with different additives to fabricate what are described as porous interconnected mullite skeletons or dense mullite bodies of approximately stoichiometric compositions. However, studies of pure fly ash examined only high-Al2O3 forms and none of these works reported long-term, high-temperature, firing shrinkage data for these mullite bodies. In the present work, high-SiO2 fly ashes were used to fabricate percolated mullite, which is demonstrated by the absence of firing shrinkage upon long-term high-temperature soaking. The major glass component of the fly ash provides viscosities suitably high for shape retention but low enough for ionic diffusion and the minor mullite component provides the nucleating agent to grow mullite needles into a direct-bonded, single-crystal, continuous, needle network that prevents high-temperature deformation and isolates the residual glass in the triple points. These attributes confer outstanding long-term dimensional stability at temperatures exceeding 1500 °C, which is unprecedented for mullite-based compositions.
Mineralogical Analysis of the Kestel Mine: An Early Bronze Age Source of Tin Ore in the Taurus Mountains, Turkey
Authors: Powell Wayne , Yazgan Evren , Johnson Michael , Yener K. Aslıhan , Mathur Ryan
Published: January 2021

Since its discovery in 1987, the Early Bronze Kestel Mine has been a topic of archaeological and geological controversy. The initial interpretation of the extensive marble-hosted galleries as the oldest known tin mine was challenged due to the low tin grade in remaining hematite-quartz veins, and it was suggested that Kestel was more likely mined for gold. Mineralogical analysis of the remaining mineralization was compared to a heavy mineral concentrate extracted from the soil preserved within the mine. The compositionally complex, arsenate-rich mineral assemblage from the mine sediment, contrasts with that of the remaining surface mineralization. Thus, the outcropping veins do not represent the nature of the extracted ore. Only one grain of gold was found in the heavy mineral concentrate, whereas cassiterite composed 1.5% of the sample. Cassiterite occurs in complex assemblages with arsenates, clays, hematite, quartz, and dolomite, bearing resemblance to hematite-arsenate tin mineralization that occurs near Kayseri, 60 km to the northeast. These findings indicate that although gold was a trace component of the Kestel ore, cassiterite was the mineral of interest to the Early Bronze Age miners, and that Kestel represents the earliest evidence thus far for an emerging pattern of local tin exploitation.
Development of in-House Industrial Fluosilicic Acid Certified Reference Material: Certification of H2SiF6 Mass Fraction
Authors: Kounbach Said , Ben Embarek Mokhtar , Chemaa Abdeljalil , Boulif Rachid , Benhida Rachid , Beniazza Redouane
Published: January 2021

Fluosilicic acid is a by-product of the chemical phosphate industry, mainly during the manufacture of phosphoric acid and triple super phosphate (TSP). To ensure the accurate measurement of the H2SiF6 mass fraction in this by-product, method validation is required, which needs a certified reference material (CRM) with its traceability to the International System of Units (SI). This work describes the development of a certified reference material of fluosilicic acid, which is commercially unavailable. Details of all steps, such as sample preparation, homogeneity and stability studies, value assignment, establishment of metrological traceability, and uncertainty estimation of the certified reference material, are fully described. The H2SiF6 mass fraction in the CRM was quantified by two analytical methods, i.e., UV-VIS as a primary method of analysis and flame mode atomic absorption spectroscopy (AAS) as a second method. It is worth noting that the results obtained from each method were in good agreement. The CRM certified value and corresponding expanded uncertainty, obtained from the combined standard uncertainty multiplied by the coverage factor (k = 2), for a confidence interval of 95%, was (91.5 ± 11.7) g·kg−1. The shelf life of the developed CRM is determined to be 1 year, provided that storage conditions are ensured. The developed CRM can be applied to validate analytical methods, improve the accuracy of measurement data as well as to establish the meteorological traceability of analytical results.
Antioxidant and Antiproliferative Activity of the Ethanolic Extract of Equisetum myriochaetum and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin
Authors: Olazarán-Santibañez Fabián , Rivera Gildardo , Vanoye-Eligio Venancio , Mora-Olivo Arturo , Aguirre-Guzmán Gabriel , Ramírez-Cabrera Mónica , Arredondo-Espinoza Eder
Published: January 2021

Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC50) value of 6.78 μg/mL against SiHa cells.