bluuebunny/crossref_metadata_2025 huggingface dataset:
Lighting a Path to the Straight and Narrow Practice of Faith Integration in Accounting
Authors: Underwood Julia
Published: May 2024

This edition of the CBAR brings its readers a rare opportunity to see the impact of one article on another article within the same publication. In Pathways to Integration: The Dialogical Approach by Underwood and Havens, a unique engagement model to faith integration is explained. The CBFA has supported Dialogical Conferences for the past four years, and we have developed and implemented five Dialogical Conferences during that period. The usefulness of the Dialogical Approach is evidenced in Implementing Faith in Accounting: Application in a Student Auditing Project Through Service to the University by LaShaw, Lambert and Sloan.
“Gain all You Can, Save all You Can, Give all You Can”: An Exercise in Giving
Authors: Tippens T. Kyle , Lynn Monty L. , Litton James D. , Brister Jozell
Published: May 2024

Theologically-informed approaches to giving are critical to faith-based business curricula, yet few resources are available for use in personal financial planning or other courses to expose students to the practice of giving. Based on a recently developed model of giving (Brister, Litton, Lynn, and Tippens, 2016), we offer background content for discussion and an exercise to catalyze thinking and practice about lifetime giving from a Christian perspective.
Impact of Student-Led Instruction vs. Professor-Led Instruction on Course Performance and Student Perceptions of Learning
Authors: Hammond Rachel
Published: May 2024

This study examines the difference in student performance when exposed to either student-led or professor-led instruction on Faith and Work topics, and whether the difference in instruction method has an impact on student beliefs about their learning and their perceptions about student-led instruction. Quiz scores and post-course survey results indicate that there was no difference in student performance based on instruction method, but student perceptions about learning varied. The results suggest that the process of creating student presentations positively influences learning, but that learning content from peers is not viewed as having a positive impact on learning.
Preparing Business Students for Participation in God’s Creative and Redemptive Purpose for the World
Authors: Windes Deborah L. , White Lynn S. , Harkema Kyle J. , Hamilton Rick A. , Samudio Courtney N.
Published: May 2024

Institutions of higher education are under an increasing amount of pressure to prove that students can justify tuition payments in terms of monetary gain. Christian liberal arts colleges, however, have a much higher calling to prepare students for service to God’s kingdom. This paper describes recent efforts by one college to combine business education with vocational calling, shaping a curriculum and assessing its effectiveness in order to better prepare students for lives of faithfulness and service.
“Grandpa, Wake Up! It’s the 21st Century!” An Opportunity for Gen Zs to Educate the Baby Boomers
Authors: Redmer Timothy A. O. , Redmer Alyssa , Redmer Kaylee , Redmer Mayah , Redmer Miriam
Published: May 2024

Baby Boomer teachers are now beginning to teach Generation Z students, many of whom were born in the 21st century. These students have grown up with state-of-the art technology and social media, which is a much different environment than many of their Baby Boomer teachers. The purpose of this paper is to compare and contrast the viewpoints of both the Baby Boomer teachers and the Generation Z students. Initially, the study focused on the characteristics of the various generations from the Silent Generation to Generation Z. This knowledge formed the basis of an empirical survey to further understand characteristics, beliefs, skills, and practices of Baby Boomers and Generation Zs and how they can better communicate with each other and coexist in an educational environment. With the COVID-19 pandemic, the real-time application of a new teaching model, using much greater technology at all levels of education, became an instant reality. The Generation Z authors were able to provide a firsthand experience in this paper on the impact of these new teaching methods on their educational experience. This paper can serve as a guide going forward on how educational institutions can effectively adapt to real-world situations in consideration of the different generations of individuals being involved.
From Data to Love: Teaching Management of Knowledge Assets In the Christian Business Classroom
Authors: Martinez Richard J.
Published: May 2024

This paper provides a roadmap of how discussion related to the nature of knowledge, the uses of knowledge, and the management of knowledge assets could be done in a Christian business classroom. I offer some of the insights I have incorporated regarding integration of our Christian faith and scripture in this discussion. What is most important for this paper is that we can envision a unique role for Christian business educators, who will have much to add to the story of knowledge management, and who will teach these processes in unique ways to students who will often use knowledge for unique purposes.
Book Review: The Way of the Shepherd: 7 Ancient Secrets to Managing Productive People
Authors: Fields W. Calvin
Published: May 2024

Book Review: The Way of the Shepherd: 7 Ancient Secrets to Managing Productive People
Spirituality in the Classroom: Two Integration Exercises For Management (and other) Classes
Authors: Smith Yvonne S.
Published: May 2024

How can a professor intentionally create space for the Spirit in a business class? One way is to use exercises and assignments that integrate a spiritual dimension into class content. This paper presents two exercises that students have found to be beneficial in “learning in righteousness.” Teaching notes are included. The exercises have been tested in management classrooms but can be adapted for many business classes.
Introducing the Ambassador Scorecard: A Christian Approach to HR Professional Excellence
Authors: Pregitzer Michael
Published: May 2024

This paper describes a framework – the Ambassador Scorecard – that Christian human resource professionals can use to help them evaluate their vocational success. Similar to other balanced scorecard methods, this framework offers a “performance dashboard” and holistic approach measuring success based on four parameters: business knowledge, human resource expertise, cultural awareness, and Christian calling. This paper discusses the key elements of the Ambassador Scorecard, describes its use in the classroom, and discusses applications to other business disciplines. Most importantly, this paper will hopefully encourage HR professionals to pursue excellence in their vocation while maintaining accountability to Christ.
The Impact of Christian Education and Curriculum on Illegal Media File Sharing Attitudes and Behavior
Authors: Lewer Joshua J. , Gerlich R. Nicholas , Lucas Doyle
Published: May 2024

The purpose of this paper is to examine the ethics and economics behind file sharing and to empirically test the role a Christian education has on illegal file sharing. The empirical results are interesting and find that Christian education has no effect on ethical attitudes or actual stealing behavior, and suggest that faculty at Christian colleges and universities cannot assume that discussions about Christian principles and moral attitudes will automatically be seen in the student behavior that follows. Integration of faith perspectives into actual practice likely needs to be intentionally addressed with specific behavioral examples as the discussion points.
Refocusing the Project Class on Partnering with the Client and Partnering with God
Authors: Harman Les
Published: May 2024

After extensive research, including a ten-year satisfaction survey, this paper presents a major shift in the pedagogy of teaching client-based project classes. Six new teaching strategies were implemented, specifically aimed at focusing marketing students on new partnering strategies to build equity in the client relationship. This resulted in superior marketing plans, highly satisfied students and clients, and a significantly greater percentage of the plan being implemented. Furthermore, spiritual integration occurred in and out of the classroom at a remarkable level as the refined class provided an ideal environment for students to partner with God.
Teaching Young Dogs Old Tricks! Management and Spiritual Lessons From America’s Most Selective School — Clown College
Authors: Sayler Larry W. , Filby Ivan L.
Published: May 2024

Our success in integrating faith and learning for a postmodern generation lies not in learning new tricks but using old evangelistic practices we learned when we first came to Christ. By doing so, we are teaching young dogs (our students) old tricks. The focus of this paper is on how one of the authors uses top 10 lists to tell his personal story to communicate business and spiritual principles. It begins with a discussion of top 10 lists and how they can be used effectively in a classroom. The main portion of the paper presents “Top 10 Lessons from America’s Most Selective College,” which communicates 10 important business and spiritual truths learned at Clown College. The final portion of the paper discusses how others might use personal stories to integrate faith and learning in the business classroom.
Preparing for the Rookie Year: Lessons from Those Who Have Gone Before
Authors: Seibert Kent W.
Published: May 2024

Christian business faculty prepare students for careers in the marketplace. But do they do enough to help students with their crucial rookie year in business? This paper proposes that faculty could do more and that the place to begin is to learn from the experiences of prior rookies. It provides an overview on the process of organizational entry and the lives of Christians in business as well as data from interviews of recent Christian college graduates who have entered the business world. The essay highlights dozens of stories of their experiences during their rookie years. Consideration is then turned to how faculty can use this information in their teaching and advising to help facilitate students’ transition from college to corporation. Finally, implications for future research are discussed.
A Radical, Reformed Christian Rejoinder to Martinez’s “Teaching Strategic Management from A Christian Perspective”
Authors: VanderVeen Steve
Published: May 2024

Dr. Richard Martinez wrote an excellent paper (which appeared in the Volume 9, 2003 issue of The JBIB) titled “Teaching Strategic Managementfrom A Christian Perspective,” examining “strategic management topics chosen according to their prominence in the leading strategic management textbooks” (Martinez, 2003, p. 71). His goal was to “spark discussion in the pursuit of developing a Christian framework for analyzing business principles” (Martinez, 2003, p. 69), and he felt his “framework [would] be amenable to further development by Christian scholars and teachers who will want to add their insights to the discussion” (Martinez, 2003, p. 95).
Measuring the Financial Transparency of Christian Colleges
Authors: Sayler Larry W. , LaShaw Margie Ness
Published: May 2024

This study measures the financial transparency of 100 U.S.-based Christian schools by assessing 1) their response to a request for IRS Form 990, 2) their response to a request for audited financial statements, and 3) whether or not they have membership in the Evangelical Council for Financial Accountability.
Internet Site: Scruples for Marketplace Christians, http://www.scruples.org
Authors: Johnson Sharon G.
Published: May 2024

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Empowered Leaders: The Ten Principles of Christian Leadership by Hans Finzel
Authors: Johnson Sharon G.
Published: May 2024

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Neither Poverty nor Riches: A Biblical Theology of Material Possessions by Craig L. Blomberg
Authors: Smith Yvonne S.
Published: May 2024

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The “Senior Blessing” at Abilene Christian University’s College of Business Administration
Authors: Fowler William , Neill John D.
Published: May 2024

The mission of Abilene Christian University (ACU) is to “educate students for Christian service and leadership throughout the world.” The universitycommunity takes this mission seriously as do we in the College of Business Administration (COBA). Not unlike most Christian business schools, we try to implement our mission by integrating faith and learningin various ways throughout the curriculum and co-curriculum. For example, we attempt to teach the various business disciplines from a Christian worldview, and many professors begin each class session by asking for the Lord’s blessing on the class. In addition, the university has a daily chapelservice that is mandatory for undergraduate students. Once a month, we have a specialized COBA chapel that is organized by students in the College of Business. Both COBA faculty and students have served as speakers during the last year. In addition to these fairly typical attempts at biblical integration, we will describe in detail in this paper one unique co-curricular activity that serves as a capstone of our attempts to integrate faith and learning. This activity is called the “Senior Blessing.”
Balancing Invitation with Intrusion in Teaching
Authors: Johnson Sharon G.
Published: May 2024

Intrude/intrusion: To thrust or force in or upon someone or something especially without permission, welcome, or fitness; to cause to enter as if by force; the act of intruding or the state of being intruded, especially: theact of wrongfully entering upon, seizing, or taking possession of the property of another; the forcible entry of molten rock or magma into or between otherrock formations.Inviting: To offer an incentive or inducement to or to entice; to increase the likelihood of; to request the presence or participation of.
God’s Economy: Teaching Students Key Biblical Principles
Authors: Surdyk Lisa Klein
Published: May 2024

Surdyk shares how she uses the Bible as a required text in her class and tells why she integrates biblical principles into her economics courses.
What Would Amos, Isaiah, and Micah Say to the Modern-Day United States? A Framework for Understanding the Economic Environment of Business in the Modern-Day United States and for Reflecting on the Events of September 11, 2001
Authors: Lemler Brad
Published: May 2024

Lemler presents a biblical integration project in which his students:1) study the ethical economic framework God set in the Pentateuch,2) observe in the Prophets the way Israel neglected God’s standard, and3) note how the behavior of the modern-day United States compares to that of Old Testament Israel.
Teaching and Learning: Connections and Distinctions
Authors: Johnson Sharon G.
Published: May 2024

I recently viewed a teacher training video in which the speaker asserted that the purpose of teaching is to cause people to learn. He argued this based on the fact that the Hebrew word for teaching and learning are the same. The Hebrew word lamad is variously translated as to learn, to study, to teach, and to train.
A Reply to Chewning’s “God is Infinitely WISE: We Have Access to His Wisdom”
Authors: Fields W. Calvin
Published: May 2024

The article “God is Infinitely WISE” by Dick Chewning looks at the epistemology of wisdom. Chewning appeals to a trilogy of concepts, including knowledge, understanding, and wisdom, in that order. Later, he elaboratesthe topic of his paper — wisdom— with further distinctions.
Work Before and After the Fall: A Project for the Managerial/Cost Accounting Course
Authors: Lemler Brad
Published: May 2024

Lemler gives an overview of how he uses a discussion on pre-fall and post-fall work to integrate faith with the managerial/cost accounting course.
Peran Guru Dalam Mengatasi Kesulitan Belajar Matematika
Authors: Mukminah Mukminah , Hirlan Hirlan , Hasanah Miswatun
Published: May 2024

Kesulitan belajar matematika siswa diketahui dari nilai siswa yang masih dibawah rata-rata, siswa tidak dapat menyelesaikan tugas dengan tepat waktu, masih kesulitan menyelesaikan dalam menjawab soal sehingga menunjukkan bahwa siswa belum menguasai materi yang diberikan oleh guru. Tujuan dari penelitian ini adalah mendeskripsikan kesulitan belajar matematika siswa kelas IV di sekolah X serta peran guru dalam mengatasi kesulitan belajar matematika siswa. Jenis penelitian ini adalah penelitian kualitatif deskriptif, subjek pada penelitian ini adalah guru dan siswa sedangkan objek pada penelitian ini adalah peran guru dalam mengatasi kesulitan belajar matematika. Tehnik pengumpulan data yang digunakan adalah observasi, wawancara dan dokumentasi. Langkah-langkah analisis data yang digunakan meliputi reduksi data, display data, dan conclusion drawing. Kesulitan belajar matematika siswa kelas IV SD di sekolah X disebabkan karena siswa memiliki karakter yang berbeda-beda diantaranya adalah kesulitan memahami simbol matematika, kesulitan dalam perhitungan dan kesulitan memahami maksud soal. Peran guru dalam mengatasi kesulitan belajar matematika siswa Guru sebagai fasilitator, pengelola, motivator, dan evaluator.
Characteristics, clinical manifestations and management of leprosy in KwaZulu-Natal, South Africa: A 20-year retrospective study
Authors: Sons J S , Mkhize Z N , Dlova N C , Aldous C , Bhat P R , Chateau A V
Published: May 2024

Background. Although leprosy, a neglected tropical disease, has been eliminated (<1 case per 10 000 population) in South Africa (SA) since 1926, according to the World Health Organization, new cases continue to be reported. The management of leprosy poses several challenges, including patient adherence, education and insufficient training of healthcare practitioners.
Objectives. To describe the biographical profile, clinical manifestations and treatment outcomes in patients with leprosy in KwaZulu-Natal Province.
Methods. This retrospective study aimed to analyse the clinical data of leprosy patients in SA from 2002 to 2022. Data collected included patient demographics, comorbidities, cutaneous and neurological manifestations of leprosy, complications, treatment and adverse reactions. Descriptive statistics were used to summarise the data.
Results. The study analysed the clinical data of 194 leprosy patients from 2002 to 2022. The majority of patients were male and middle aged, with a disproportionate representation of black South Africans. Regarding socioeconomic status, 80% were unemployed and 40% were social grant recipients. Most cases were clustered in urban centres and diagnosed at secondary care facilities, with 15% being HIV positive. The majority of patients (90%) were classified as having multibacillary leprosy. Common symptoms included upper respiratory tract involvement, hair loss and painful nerves, with the face and limbs being most frequently affected. Cutaneous morphology predominantly included plaques and hypopigmented patches, while neurological signs included ulnar nerve tenderness, muscle weakness and sensory deficits. Debilitating neurological complications were found in one-fifth of patients. Despite initiation of multidrug therapy in most patients, a significant proportion (27.3%) did not complete the full course of treatment, and treatment reactions were noted in 33.5% of patients.
Conclusion. These findings emphasise the urgent need for enhanced patient and healthcare worker education, particularly in primary healthcare settings, to improve adherence to treatment, advocate for prophylactic measures and prevent new cases. Achieving leprosy-free status in SA requires the collaboration of many role-players to address these challenges and improve healthcare practices.
Will South Africa meet the Sustainable Development Goals target for maternal mortality by 2030?
Authors: Pillay Y , Moodley J M
Published: May 2024

In September 2015, South Africa (SA) and 192 countries adopted Agenda 2030, which included the Sustainable Development Goals. With a mere 6 years to go before 2030, it is useful to understand what progress SA is making towards their attainment. In this short report, we assess progress towards meeting the maternal mortality target, globally and in SA. The maternal mortality ratio that countries are expected to reach is no more than 70 deaths per 100 000 live births. A range of sources is used to show progress, with an emphasis on the reports of the National Committee on Confidential Enquiries into Maternal deaths, which reports on the number of maternal deaths in health facilities, together with reasons for these deaths and recommendations to reduce preventable mortality.
Zombis del tecnoliberalismo y del régimen ‘live’: ¡danzad, danzad, malditos!
Authors: Herrera Sánchez Sonia
Published: May 2024

Son muchas las visiones críticas sobre el capitalismo digital que se están elaborando en los últimos años desde diferentes disciplinas y saberes, advertencias que hay que tener en cuenta y que hay que poner en diálogo. En medio de la infoxicación de las redes, ¿nos estaremos convirtiendo en siervos y siervas zombificados de un nuevo régimen?
SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
Authors: Forcella Pascal , Ifflander Niklas , Rolando Chiara , Balta Elli-Anna , Lampada Aikaterini , Giachino Claudio , Mukhtar Tanzila , Bock Thomas , Taylor Verdon
Published: May 2024

Neural stem cells (NSCs) are multipotent and correct fate determination is crucial to guarantee brain formation and homeostasis. How NSCs are instructed to generate neuronal or glial progeny is not well understood. Here, we addressed how murine adult hippocampal NSC fate is regulated and described how scaffold attachment factor B (SAFB) blocks oligodendrocyte production to enable neuron generation. We found that SAFB prevents NSC expression of the transcription factor nuclear factor I/B (NFIB) by binding to sequences in the Nfib mRNA and enhancing Drosha-dependent cleavage of the transcripts. We show that increasing SAFB expression prevents oligodendrocyte production by multipotent adult NSCs, and conditional deletion of Safb increases NFIB expression and oligodendrocyte formation in the adult hippocampus. Our results provide novel insights into a mechanism that controls Drosha functions for selective regulation of NSC fate by modulating the post-transcriptional destabilization of Nfib mRNA in a lineage-specific manner.
Pigmentation level of human iPSC-derived RPE does not indicate a specific gene expression profile
Authors: Nakai-Futatsugi Yoko , Jin Jianshi , Ogawa Taisaku , Sakai Noriko , Maeda Akiko , Hironaka Ken-ichi , Fukuda Masakazu , Danno Hiroki , Tanaka Yuji , Hori Seiji , Shiroguchi Katsuyuki , Takahashi Masayo
Published: May 2024

Retinal pigment epithelium (RPE) cells show heterogeneous levels of pigmentation when cultured in vitro. To know whether their color in appearance is correlated with the function of the RPE, we analyzed the color intensities of human-induced pluripotent stem cell-derived RPE cells (iPSC-RPE) together with the gene expression profile at the single-cell level. For this purpose, we utilized our recent invention, Automated Live imaging and cell Picking System (ALPS), which enabled photographing each cell before RNA-sequencing analysis to profile the gene expression of each cell. While our iPSC-RPE were categorized into four clusters by gene expression, the color intensity of iPSC-RPE did not project any specific gene expression profiles. We reasoned this by less correlation between the actual color and the gene expressions that directly define the level of pigmentation, from which we hypothesized the color of RPE cells may be a temporal condition not strongly indicating the functional characteristics of the RPE.
Evolution of olfactory sensitivity, preferences and behavioral responses in Mexican cavefish: fish personality matters
Authors: Blin Maryline , Valay Louis , Kuratko Manon , Pavie Marie , Rétaux Sylvie
Published: May 2024

Animals are adapted to their natural habitats and lifestyles. Their brains perceive the external world
via
their sensory systems, compute information together with that of internal states and autonomous activity, and generate appropriate behavioral outputs. However, how do these processes evolve across evolution? Here, focusing on the sense of olfaction, we have studied the evolution in olfactory sensitivity, preferences and behavioral responses to six different food-related amino acid odors in the two eco-morphs of the fish
Astyanax mexicanus
. To this end, we have developed a high-throughput behavioral setup and pipeline of quantitative and qualitative behavior analysis, and we have tested 489 six-week-old
Astyanax
larvae. The blind, dark-adapted morphs of the species showed markedly distinct basal swimming patterns and behavioral responses to odors, higher olfactory sensitivity and a strong preference for alanine, as compared to their river-dwelling eyed conspecifics. In addition, we discovered that fish have an individual “swimming personality”, and that this personality influences their capability to respond efficiently to odors and find the source. Importantly, the personality traits that favored significant responses to odors were different in surface fish and cavefish. Moreover, the responses displayed by second-generation cave x surface F2 hybrids suggested that olfactory-driven behavior and olfactory sensitivity is a quantitative genetic trait. Our findings show that olfactory processing has rapidly evolved in cavefish at several levels: detection threshold, odor preference, and foraging behavior strategy. Cavefish is therefore an outstanding model to understand the genetic, molecular and neurophysiological basis of sensory specialization in response to environmental change.
Sequence characteristics and an accurate model of abundant hyperactive loci in the human genome
Authors: Hudaiberdiev Sanjarbek , Ovcharenko Ivan
Published: May 2024

Enhancers and promoters are classically considered to be bound by a small set of TFs in a sequence-specific manner. This assumption has come under increasing skepticism as the datasets of ChIP-seq assays of TFs have expanded. In particular, high-occupancy target (HOT) loci attract hundreds of TFs with seemingly no detectable correlation between ChIP-seq peaks and DNA-binding motif presence. Here, we used a set of 1,003 TF ChIP-seq datasets (HepG2, K562, H1) to analyze the patterns of ChIP-seq peak co-occurrence in combination with functional genomics datasets. We identified 43,891 HOT loci forming at the promoter (53%) and enhancer (47%) regions. HOT promoters regulate housekeeping genes, whereas HOT enhancers are involved in tissue-specific process regulation. HOT loci form the foundation of human super-enhancers and evolve under strong negative selection, with some of these loci being located in ultraconserved regions. Sequence-based classification analysis of HOT loci suggested that their formation is driven by the sequence features, and the density of mapped ChIP-seq peaks across TF-bound loci correlates with sequence features and the expression level of flanking genes. Based on the affinities to bind to promoters and enhancers we detected 5 distinct clusters of TFs that form the core of the HOT loci. We report an abundance of HOT loci in the human genome and a commitment of 51% of all TF ChIP-seq binding events to HOT locus formation thus challenging the classical model of enhancer activity and propose a model of HOT locus formation based on the existence of large transcriptional condensates.
Purging viral latency by a bifunctional HSV-vectored therapeutic vaccine in chronically SIV-infected macaques
Authors: Wen Ziyu , Li Pingchao , Yuan Yue , Wang Congcong , Li Minchao , Wang Haohang , Shi Minjuan , He Yizi , Cui Mingting , Chen Ling , Sun Caijun
Published: May 2024

The persistence of latent viral reservoirs remains the major obstacle to eradicating human immunodeficiency virus (HIV). We herein found that ICP34.5 can act as an antagonistic factor for the reactivation of HIV latency by herpes simplex virus type I (HSV-1), and thus recombinant HSV-1 with ICP34.5 deletion could more effectively reactivate HIV latency than its wild-type counterpart. Mechanistically, HSV-ΔICP34.5 promoted the phosphorylation of HSF1 by decreasing the recruitment of protein phosphatase 1 (PP1α), thus effectively binding to the HIV LTR to reactivate the latent reservoirs. In addition, HSV-ΔICP34.5 enhanced the phosphorylation of IKKα/β through the degradation of IκBα, leading to p65 accumulation in the nucleus to elicit NF-κB pathway-dependent reactivation of HIV latency. Then, we constructed the recombinant HSV-ΔICP34.5 expressing simian immunodeficiency virus (SIV) env, gag, or the fusion antigen sPD1-SIVgag as a therapeutic vaccine, aiming to achieve a functional cure by simultaneously reactivating viral latency and eliciting antigen-specific immune responses. Results showed that these constructs effectively elicited SIV-specific immune responses, reactivated SIV latency, and delayed viral rebound after the interruption of antiretroviral therapy (ART) in chronically SIV-infected rhesus macaques. Collectively, these findings provide insights into the rational design of HSV-vectored therapeutic strategies for pursuing an HIV functional cure.
Purging viral latency by a bifunctional HSV-vectored therapeutic vaccine in chronically SIV-infected macaques
Authors: Wen Ziyu , Li Pingchao , Yuan Yue , Wang Congcong , Li Minchao , Wang Haohang , Shi Minjuan , He Yizi , Cui Mingting , Chen Ling , Sun Caijun
Published: May 2024

The persistence of latent viral reservoirs remains the major obstacle to eradicating human immunodeficiency virus (HIV). We herein reported that recombinant herpes simplex virus type I (HSV-1) with ICP34.5 deletion could more effectively reactivate HIV latency than its wild-type counterpart. Mechanistically, HSV-ΔICP34.5 promoted the phosphorylation of HSF1 by decreasing the recruitment of protein phosphatase 1 (PP1α), thus effectively binding to the HIV LTR to reactivate the latent reservoirs. In addition, HSV-ΔICP34.5 enhanced the phosphorylation of IKKα/β through the degradation of IκBα, leading to p65 accumulation in the nucleus to elicit NF-κB pathway-dependent reactivation of HIV latency. Then, we constructed the recombinant HSV-ΔICP34.5 expressing simian immunodeficiency virus (SIV) env, gag, or the fusion antigen sPD1-SIVgag as an HIV therapeutic vaccine, aiming to achieve a functional cure by simultaneously reactivating viral latency and eliciting antigen-specific immune responses. Results showed that these constructs effectively elicited SIV-specific immune responses, reactivated SIV latency, and delayed viral rebound after the interruption of antiretroviral therapy (ART) in chronically SIV-infected rhesus macaques. Collectively, these findings provide insights into the rational design of HSV-vectored therapeutic strategies for pursuing an HIV functional cure.
Direct modulation of TRPM8 ion channels by rapamycin and analog macrolide immunosuppressants
Authors: Tóth Balázs István , Bazeli Bahar , Janssens Annelies , Lisztes Erika , Racskó Márk , Kelemen Balázs , Herczeg Mihály , Nagy Tamás Milán , Kövér Katalin E. , Mitra Argha , Borics Attila , Bíró Tamás , Voets Thomas
Published: May 2024

Rapamycin (sirolimus), a macrolide compound isolated from the bacterium
Streptomyces hygroscopicus
, is widely used as oral medication for the prevention of transplant rejection and the treatment of lymphangioleiomyomatosis. It is also incorporated in coronary stent coatings to prevent restenosis and in topical preparations for the treatment of skin disorders. Rapamycin’s
in vivo
activities are generally ascribed to its binding to the protein FKBP12, leading to potent inhibition of the mechanistic target of rapamycin kinase (mTOR) by the FKBP12-rapamycin complex. The specific rapamycin-induced interaction between domains from mTOR and FKBP12 is also frequently employed in cell biological research, for rapid chemically-induced protein dimerization strategies. Here we show that rapamycin activates TRPM8, a cation channel expressed in sensory nerve endings that serves as the primary cold sensor in mammals. Using a combination of electrophysiology, Saturation Transfer Triple-Difference (STTD) NMR spectroscopy and molecular docking-based targeted mutagenesis, we demonstrate that rapamycin directly binds to TRPM8. We identify a rapamycin-binding site in the groove between voltage sensor-like domain and the pore domain, distinct from the interaction sites of cooling agents and known TRPM8 agonists menthol and icilin. Related macrolide immunosuppressants act as partial TRPM8 agonists, competing with rapamycin for the same binding site. These findings identify a novel molecular target for rapamycin and provide new insights into the mechanisms of TRPM8 activation, which may assist in the development of therapies targeting this ion channel. Moreover, our findings also indicate that caution is needed when using molecular approaches based on rapamycin-induced dimerization to study ion channel regulation.
Two long-axis dimensions of hippocampal-cortical integration support memory function across the adult lifespan
Authors: Nordin Kristin , Pedersen Robin , Falahati Farshad , Johansson Jarkko , Grill Filip , Andersson Micael , Korkki Saana M. , Bäckman Lars , Zalesky Andrew , Rieckmann Anna , Nyberg Lars , Salami Alireza
Published: May 2024

The hippocampus is a complex structure critically involved in numerous behavior-regulating systems. In young adults, multiple overlapping spatial modes along its longitudinal and transverse axes describe the organization of its functional integration with neocortex, extending the traditional framework emphasizing functional differences between sharply segregated hippocampal subregions. Yet, it remains unknown whether these modes (i.e., gradients) persist across the adult human lifespan, and relate to memory and molecular markers associated with brain function and cognition. In two independent samples, we demonstrate that the principal anteroposterior and second-order, mid-to-anterior/posterior hippocampal modes of neocortical functional connectivity, representing distinct dimensions of macroscale cortical organization, manifest across the adult lifespan. Specifically, individual differences in topography of the second-order gradient predicted episodic memory and mirrored dopamine D1 receptor distribution, capturing shared functional and molecular organization. Older age was associated with less distinct transitions along gradients (i.e., increased functional homogeneity). Importantly, a youth-like gradient profile predicted preserved episodic memory – emphasizing age-related gradient dedifferentiation as a marker of cognitive decline. Our results underscore a critical role of mapping multidimensional hippocampal organization in understanding the neural circuits that support memory across the adult lifespan.
The effect of social anxiety on threat acquisition and extinction: a systematic review and meta-analysis
Authors: Wake Shannon , Hedger Nicholas , van Reekum Carien M. , Dodd Helen
Published: May 2024

Although exposure-based therapy has been found to be effective at alleviating symptoms of social anxiety disorder, it often does not lead to full remission, and relapse after treatment is common. Exposure therapy is based on theoretical principles of extinction of conditioned fear responses. However, there are inconsistencies in findings across experiments that have investigated the effect of social anxiety on threat conditioning and extinction processes. This systematic review and meta-analysis aimed to examine whether elevated levels of social anxiety are associated with abnormalities in threat conditioning and extinction processes. A second aim was to examine the sensitivity of various study designs and characteristics to detect social anxiety-related differences in threat conditioning and extinction. A systematic search was conducted, which identified twenty-three experiments for inclusion in the review. The findings did not demonstrate compelling evidence that high levels of social anxiety are associated with atypical threat conditioning or extinction. Further, when systematically examining the data, there was no convincing support that the use of a particular psychophysiological measure, subjective rating, or experimental parameter yields more consistent associations between social anxiety and conditioning processes during threat acquisition or extinction. Meta-analyses demonstrated that during threat extinction, the use of anxiety ratings as a dependent variable, socially relevant unconditioned stimuli, and a higher reinforcement schedule produced more detectable effects of social anxiety on compromised extinction processes compared to any other dependent variable (subjective or physiological) or experimental parameter. Overall, the results of this study suggest that social anxiety is not reliably related to deficits in conditioning and extinction processes in the context of laboratory-based Pavlovian conditioning paradigms.
Regulation of cellular and molecular markers of epithelial-mesenchymal transition by Brazilin in breast cancer cells
Authors: Cayetano-Salazar Lorena , Hernandez-Moreno Jose A. , Bello-Martinez Jorge , Olea-Flores Monserrat , Castañeda-Saucedo Eduardo , Ramirez Monica , Mendoza-Catalán Miguel A. , Navarro-Tito Napoleon
Published: May 2024

Breast cancer is the most common invasive neoplasm and the leading cause of cancer death in women worldwide. The main cause of mortality in cancer patients is invasion and metastasis, where the epithelial-mesenchymal transition (EMT) is a crucial player in these processes. Pharmacological therapy has plants as its primary source, including isoflavonoids. Brazilin is an isoflavonoid isolated from Haematoxilum brasiletto that has shown antiproliferative activity in several cancer cell lines. In this study, we evaluated the effect of Brazilin on canonical markers of EMT such as E-cadherin, vimentin, Twist, and matrix metalloproteases (MMPs). By Western blot, we evaluated E-cadherin, vimentin, and Twist expression and the subcellular localization by immunofluorescence. Using gelatin zymography, we determined the levels of secretion of MMPs. We used Transwell chambers coated with matrigel to determine the in vitro invasion of breast cancer cells treated with Brazilin. Interestingly, our results show that Brazilin increases 50% in E-cadherin expression and decreases 50% in vimentin and Twist expression, MMPs, and cell invasion in triple-negative breast cancer (TNBC) MDA-MB-231 and to a lesser extend in MCF7 ER+ breast cancer cells. Together, these findings position Brazilin as a new molecule with great potential for use as complementary or alternative treatment in breast cancer therapy in the future.
ROL DE ENFERMERÍA EN PESQUISA PRECOZ DEL TRASTORNO DEL ESPECTRO AUTISTA EN NIÑOS: REVISIÓN NARRATIVA
Authors: Hernández Díaz Alejandro , Sepúlveda Pérez Mauricio , Castro Sanhueza María Fernanda
Published: May 2024

INTRODUCCIÓN: El quehacer de la enfermería cumple un rol fundamental en el proceso de detección temprana de Autismo, por ello debe poner en práctica sus habilidades profesionales enfocada en la familia desplegando diferentes roles disciplinares. OBJETIVO: Analizar la evidencia científica disponible sobre el rol de enfermería en la pesquisa precoz del trastorno del espectro autista. METODOLOGÍA: Se realizó una revisión narrativa, la investigación se realizó desde julio 2022 hasta abril del 2023, los textos incluidos fueron en idioma español, inglés y portugués. Se utilizaron 4 bases de datos: Pubmed, Scielo, CUIDEN, y Dialnet. RESULTADOS: Fueron encontrados y evaluados 146 estudios de acuerdo a criterios de inclusión y exclusión de ello emergieron 6 artículos finalmente identificados, 4 americanos, 1 japonés y 1 europeo, todos vinculados con la temática a investigar propuestos. CONCLUSIONES: Del rol educativo: el más mencionado y reconocido por la disciplina, se evidencia asociación intrínseca hacia la familia, se menciona carencias formativas en el curriculum universitario, se menciona innovar en tecnologías educativas; del rol gestión: se valida el instrumento M-Chat como acción ejecutiva y evaluativa reconocida por enfermeras, pero se menciona falta de estandarizaciones técnicas, se reconoce poca vinculación intersectorial, falta nivelar protocolos de actuación; del rol asistencial: destaca intervenciones en identificación de signos tempranos, emerge coacción multidisciplinaria, clave es afrontamiento familiar; del rol investigativo: falta desarrollo y ampliar publicaciones; emerge dimensión ética bien vista por enfermeras poco mencionada por familia, se destaca labor de acompañamiento y cuidados humanizados siempre ligado a gestión.
IMPLEMENTACIÓN DE METODOLOGÍAS ACTIVO-PARTICIPATIVAS PARA EL APRENDIZAJE SIGNIFICATIVO DEL CUIDADO HUMANIZADO EN LA CARRERA DE ENFERMERÍA
Authors: Hormazábal Lara Jonás , Ortega Ortega Tamara , Ureta Narváez Inay
Published: May 2024

La ley 20.584 que alude a los derechos y deberes que tienen las personas en relación con acciones vinculadas a la atención de salud, menciona que un derecho del usuario es recibir un trato digno a partir del personal de salud. El cumplimento de este derecho va en desmedro, en base a los antecedentes recopilados sobre la situación de la carrera de enfermería en Chile, donde éstos evidencian que existe insatisfacción usuaria con respecto a la atención específicamente de esta área, además de la escasa interacción enfermera/o - usuario debido a la pandemia COVID-19 (2020-2022), por el uso de aislamientos y elementos de protección personal. Esta situación abre la necesidad de hacer hincapié en intervenir las metodologías de enseñanza-aprendizaje que se imparten en las carreras de enfermería del país de diferentes instituciones de educación superior con énfasis en el cuidado humanizado. El siguiente estudio tiene como objetivo aplicar metodologías activo-participativas para la adquisición del aprendizaje significativo del cuidado humanizado en la asignatura de enfermería en salud mental de la carrera de enfermería de una universidad chilena en el segundo semestre del año 2022. Se realizó un estudio en base al modelo de investigación-acción, a 68 estudiantes de la asignatura aplicando herramientas metodológicas para el aprendizaje y tecnologías de la información y las comunicaciones (TICS). Los resultados del proyecto evidencian conocimiento destacado y satisfactorio sobre el cuidado humanizado de 82% (prepráctica clínica) a 90,2% (post práctica clínica) posterior a la aplicación del proyecto, aportando a profundizar el conocimiento y mejorar la relación terapéutica con el usuario logrando efectividad en el proceso de atención de salud, desde el rol del estudiante de enfermería.
IMPLEMENTACIÓN DE LAS GUÍAS DE LA RNAO EN LA CARRERA DE ENFERMERÍA DE LA UNIVERSIDAD AUTÓNOMA DE CHILE
Authors: Benavides-Roca María , Sirguiado Davis Marcela , Núñez-González Adrian , Benavides-Roca Luis
Published: May 2024

La enfermería basada en evidencia se posiciona como una alternativa fiable ante las necesidades actuales de la enfermería, a partir de eso las guías de buenas prácticas de la RNAO (Asociación Profesional de Enfermeras de Ontario) son una herramienta optimizadora del sistema de salud, abarcando las áreas clínicas y académicas de la enfermería. El objetivo del presente estudio es describir la experiencia de la implementación de las guías de buenas prácticas de la RNAO en el pregrado de la carrera de Enfermería de la Universidad Autónoma de Chile, sede Talca. Este artículo reflexiona sobre el fortalecimiento del modelo curricular de la carrera de enfermería a partir de la implementación de un marco para la acción que se encuentra respaldado por la evidencia científica del programa de Best Practice Spot-light Organizations (BPSO®).
CALIDAD Y SEGURIDAD ASISTENCIAL: LESIONES POR PRESIÓN UN INDICADOR SENSIBLE DE CUIDADO Y BRECHA PENDIENTE EN LA POLÍTICA PÚBLICA DE SALUD
Authors: Pinilla Carrasco Nicole
Published: May 2024

INTRODUCCIÓN: Las úlceras por presión, actualmente llamadas lesiones, son un problema de salud pública de gran impacto, especialmente en personas con dependencia severa, donde el inmovilismo es común. A nivel mundial, las LPP representan millones de eventos adversos anuales, con miles de casos con resultado de muerte. OBJETIVO: Reflexionar sobre la persistencia de las lesiones por presión, a pesar de las medidas preventivas desarrolladas. Se busca destacar la importancia ética de abordar esta problemática en la Atención Primaria, especialmente en el cuidado domiciliario, y abogar por el desarrollo de evidencia objetiva para respaldar la implementación de nuevas políticas públicas orientadas a la vigilancia y prevención. CONCLUSIONES: La lesiones por presión es un problema persistente en Chile, siendo considerada un evento centinela prevenible. Aunque existen regulaciones en el sistema sanitario, la atención domiciliaria, parte integral de la atención primaria de salud, se ve en desmedro. El cuidado perdido, donde las intervenciones de enfermería no se realizan, aumenta el riesgo de lesiones por presión por lo que es crucial capacitar al personal de enfermería para seguir el plan de cuidado y promover una cultura de prevención. Las lesiones por presión, tienen un impacto negativo en los individuos y el sistema de salud, generando costos adicionales y sobrecarga en los niveles de atención. Se necesita una revisión de la normativa para incluir directrices específicas para la atención domiciliaria, desarrollar protocolos adaptados y proporcionar capacitación adecuada. Esto garantizaría una atención más integral, reduciría la carga sobre el sistema de salud y promovería la equidad en el acceso a la atención de calidad.
MADELEINE LEININGER COMO REFERENTE METODOLÓGICO DE LA ETNOENFERMERÍA
Authors: Briñez Ariza Karol Johanna , Ortiz Whitaker María Carolina
Published: May 2024

INTRODUCCIÓN: La etnoenfermería se considera como un método propio de enfermería para investigar el cuidado. Se identificó en la literatura académica en español una necesidad de describir la propuesta metodológica de Leininger como referente para la investigación en enfermería. El cuidado culturalmente congruente invita a reconocer las dimensiones de la estructura cultural y social como determinantes para la salud, y así entender comportamientos en salud y respuestas de las personas. OBJETIVO: describir la propuesta metodológica de la etnoenfermería. REFLEXIÓN: a partir de tres preguntas orientadoras se indaga la comprensión de los fenómenos respondidos desde la etnoenfermería con articulación entre enfermería-cuidado-cultura y claridad en el método de etnoenfermería propuesto por Leininger. CONCLUSIÓN: plantear en las investigaciones a Leininger como referente metodológico permite encontrar articulación en los fenómenos culturales que están involucrados en los procesos salud-enfermedad, se requiere conocimiento metodológico y teórico de la propuesta de Leininger para brindar cuidado culturalmente congruente.
Análise do sistema logístico em uma empresa de óleo de palma situada em Tomé Açu/PA
Authors: Carmo Thairla da Graça , Albuquerque Mikely Gomes , Santos Jordy Lopes dos , Brandão Rayra , Drago Ivana Ribeiro , Baia Simone Andrea Lima do Nascimento
Published: May 2024

Os processos de logística são inerentes as organizações que precisam dispor de materiais na quantidade certa, no lugar certo, com qualidade adequada, no momento certo e a um preço razoável e acessível afim de diminuir otimizar custos com foco na satisfação do cliente. O presente trabalho buscou demonstrar a importância do sistema logístico em uma empresa de óleo de palma localizada em Tomé Açu/PA. A metodologia utilizada foi por meio de um estudo de caso de natureza qualitativa, bem como utiliza-se de análises de conteúdo bibliográfico, possuindo caráter explicativo. Cabe ressaltar que é preciso ter um processo da cadeia de suprimentos em que haja eficiência desde o momento inicial da produção até o processo final.