bluuebunny/crossref_metadata_2025 huggingface dataset:
Fruit growth and development in apple: a molecular, genomics and epigenetics perspective
Authors: Jahed Khalil R. , Hirst Peter M.
Published: April 2023

Fruit growth and development are physiological processes controlled by several internal and external factors. This complex regulatory mechanism comprises a series of events occurring in a chronological order over a growing season. Understanding the underlying mechanism of fruit development events, however, requires consideration of the events occurring prior to fruit development such as flowering, pollination, fertilization, and fruit set. Such events are interrelated and occur in a sequential order. Recent advances in high-throughput sequencing technology in conjunction with improved statistical and computational methods have empowered science to identify some of the major molecular components and mechanisms involved in the regulation of fruit growth and have supplied encouraging successes in associating genotypic differentiation with phenotypic observations. As a result, multiple approaches have been developed to dissect such complex regulatory machinery and understand the genetic basis controlling these processes. These methods include transcriptomic analysis, quantitative trait loci (QTLs) mapping, whole-genome approach, and epigenetics analyses. This review offers a comprehensive overview of the molecular, genomic and epigenetics perspective of apple fruit growth and development that defines the final fruit size and provides a detailed analysis of the mechanisms by which fruit growth and development are controlled. Though the main emphasis of this article is on the molecular, genomic and epigenetics aspects of fruit growth and development, we will also deliver a brief overview on events occurring prior to fruit growth.
Tuber indicum and T. lijiangense colonization differentially regulates plant physiological responses and mycorrhizosphere bacterial community of Castanopsis rockii seedlings
Authors: Huang Lanlan , Li Yongmei , Yuan Jing , Wan Shanping , Colinas Carlos , He Xinhua , Shi Xiaofei , Wang Yanliang , Yu Fuqiang
Published: April 2023

Black truffles and white truffles are widely studied around the world, but their effects on plant growth and physiological responses, and on the mycorrhizosphere bacterial community of the host plant remain unclear. Here, mycorrhizal colonization of Castanopsis rockii by Tuber indicum (Chinese black truffle) and T. lijiangense (Chinese white truffle), respectively, was induced in a greenhouse study, and their effects on host growth, physiological responses and mycorrhizosphere bacterial communities were compared. The results show that colonization of both Tuber species significantly increased leaf photosynthetic rate, leaf P concentration and mycorrhizosphere acid phosphatase activity, as well as richness of mycorrhizosphere bacterial communities of C. rockii seedlings. However, T. indicum colonization on the one hand significantly decreased tartrate content, bacterial acid phosphatase, phoC gene abundance in the mycorrhizosphere, and peroxidase (POD) activity of ectomycorrhizal root tips, but on the other hand increased mycorrhizosphere pH and superoxide dismutase (SOD) of ectomycorrhizal root tips, compared to T. lijiangense colonization. Moreover, principal coordinate and β-diversity analyses show significant differences in mycorrhizosphere bacterial community composition between T. indicum and T. lijiangese colonized C. rockii seedlings. Finally, the relative abundance of the bacterium Agromyces cerinus significantly correlated to mycorrhizosphere acid phosphatase activity and leaf P concentration, suggesting that this bacterium might play an important role in P mobilization and acquisition. Overall, these results suggest that T. indicum and T. lijiangense differently regulate their host plant’s physiological responses and mycorrhizosphere bacterial community.
Genome-wide association analysis revealed genetic variation and candidate genes associated with the yield traits of upland cotton under drought conditions
Authors: Sun Fenglei , Ma Jun , Shi Weijun , Yang Yanlong
Published: April 2023

Drought is one of the major abiotic stresses seriously affecting cotton yield. At present, the main cotton-producing areas in China are primarily arid and semiarid regions. Therefore, the identification of molecular markers and genes associated with cotton yield traits under drought conditions is of great importance for stabilize cotton yield under such conditions. In this study, resequencing data were used to conduct a genome-wide association study (GWAS) on 8 traits of 150 cotton germplasms. Under drought stress, 18 SNPs were significantly correlated with yield traits (single-boll weight (SBW) and seed (SC)), and 8 SNPs were identified as significantly correlated with effective fruit shoot number (EFBN) traits (a trait that is positively correlated with yield). Finally, a total of 15 candidate genes were screened. The combined results of the GWAS and transcriptome data analysis showed that four genes were highly expressed after drought stress, and these genes had significantly increased expression at 10, 15 and 25 DPA of fiber development. qRT-PCR was performed on two samples with drought tolerance extremes (drought-resistant Xinluzao 45 and drought-sensitive Xinluzao 26), revealing that three of the genes had the same differential expression pattern. This study provides a theoretical basis for the genetic analysis of cotton yield traits under drought stress, and provides gene resources for improved breeding of cotton yield traits under drought stress.
Phenylpropanoid amides from Solanum rostratum and their phytotoxic activities against Arabidopsis thaliana
Authors: Liu Zhixiang , Ma Xiaoqing , Zhang Nan , Yuan Linlin , Yin Hongrui , Zhang Lingling , An Tong , Xu Yubin
Published: April 2023

Introduction

Solanum rostratum, an annual malignant weed, has seriously damaged the ecological environment and biodiversity of invasion area. This alien plant gains a competitive advantage by producing some new phytotoxic substances to inhibit the growth of native plants, thus achieving successful invasion. However, the chemical structures, inhibitory functions and action mechanisms of phytotoxic substances of S. rostratum remain unclear.

Methods

In this study, to clarify the chemical structures of phytotoxic substances from S. rostratum, we isolated phenylpropanoid amides from the plant. Their structures were identified by comprehensive HR-ESIMS, NMR and ECD data. And the inhibitory functions of isolated phenylpropanoid amides on one model plant (Arabidopsis thaliana) were also investigated. In addition, the action mechanisms of active phenylpropanoid amides were revealed by antioxidant-related enzymes [Catalase (CAT), Peroxidase (POD), Superoxide dismutase (SOD)] activities and corresponding molecular docking analyses.

Results and Discussion

Phytochemical research on the whole plant of S. rostratum led to the isolation and identification of four new phenylpropanoid amides (1−4), together with two known analogues (5−6). All the compounds showed phytotoxic effects with varying levels on the seed germination and root elongation of one model plant (Arabidopsis thaliana), especially compound 2 and 4. Likewise, compounds 2 and 4 displayed potent inhibitory effects on antioxidant-related enzyme (POD). In addition, compounds 2 and 4 formed common conventional hydrogen bonds with residues Ala34 and Ser35 in POD revealed by molecular docking analyses. These findings not only helped to reveal the invasion mechanism of S. rostratum from the perspective of “novel weapons hypothesis”, but also opened up new ways for the exploitation and utilization of S. rostratum.
Restraint stress induced anxiety and sleep in mice
Authors: Xu Yong-Xia , Liu Guo-Ying , Ji Zhang-Zhang , Li Yue-Yun , Wang Yan-Li , Wu Xue-Yan , Liu Jun-Lin , Ma Dan-Xia , Zhong Ming-Kui , Gao Chao-Bing , Xu Qi
Published: April 2023

In humans and animals, exposure to changes in internal or external environments causes acute stress, which changes sleep and enhances neurochemical, neuroendocrine, and sympathetic activities. Repeated stress responses play an essential role in the pathogenesis of psychiatric diseases and sleep disorders. However, the underlying mechanism of sleep changes and anxiety disorders in response to acute stress is not well established. In the current study, the effects of restraint stress (RS) on anxiety and sleep–wake cycles in mice were investigated. We found that after RS, the mice showed anxiety-like behavior after RS manipulation and increased the amounts of both non-rapid eye movement (NREM) and rapid eye movement (REM) sleep in the dark period. The increase in sleep time was mainly due to the increased number of episodes of NREM and REM sleep during the dark period. In addition, the mice showed an elevation of the EEG power spectrum of both NREM and REM sleep 2 h after RS manipulation. There was a significant reduction in the EEG power spectrum of both NREM and REM sleep during the darkperiod in the RS condition. The expression of the c-Fos protein was significantly increased in the parabrachial nucleus, bed nucleus of the stria terminalis, central amygdala, and paraventricular hypothalamus by RS manipulation. Altogether, the findings from the present study indicated that neural circuits from the parabrachial nucleus might regulate anxiety and sleep responses to acute stress, and suggest a potential therapeutic target for RS induced anxiety and sleep alterations.
Effectiveness of non-pharmaceutical interventions in nine fields of activity to decrease SARS-CoV-2 transmission (Spain, September 2020–May 2021)
Authors: Barbeito Inés , Precioso Daniel , Sierra María José , Vegas-Azcárate Susana , Fernández Balbuena Sonia , Vitoriano Begoña , Goméz-Ullate David , Cao Ricardo , Monge Susana ,   the Study Group for Non-Pharmaceutical Interventions in Spain
Published: April 2023

Background

We estimated the association between the level of restriction in nine different fields of activity and SARS-CoV-2 transmissibility in Spain, from 15 September 2020 to 9 May 2021.

Methods

A stringency index (0–1) was created for each Spanish province (n = 50) daily. A hierarchical multiplicative model was fitted. The median of coefficients across provinces (95% bootstrap confidence intervals) quantified the effect of increasing one standard deviation in the stringency index over the logarithmic return of the weekly percentage variation of the 7-days SARS-CoV-2 cumulative incidence, lagged 12 days.

Results

Overall, increasing restrictions reduced SARS-CoV-2 transmission by 22% (RR = 0.78; one-sided 95%CI: 0, 0.82) in 1 week, with highest effects for culture and leisure 14% (0.86; 0, 0.98), social distancing 13% (0.87; 0, 0.95), indoor restaurants 10% (0.90; 0, 0.95) and indoor sports 6% (0.94; 0, 0.98). In a reduced model with seven fields, culture and leisure no longer had a significant effect while ceremonies decreased transmission by 5% (0.95; 0, 0.96). Models R2 was around 70%.

Conclusion

Increased restrictions decreased COVID-19 transmission. Limitations include remaining collinearity between fields, and somewhat artificial quantification of qualitative restrictions, so the exact attribution of the effect to specific areas must be done with caution.
Progress in biological age research
Authors: Li Zhe , Zhang Weiguang , Duan Yuting , Niu Yue , Chen Yizhi , Liu Xiaomin , Dong Zheyi , Zheng Ying , Chen Xizhao , Feng Zhe , Wang Yong , Zhao Delong , Sun Xuefeng , Cai Guangyan , Jiang Hongwei , Chen Xiangmei
Published: April 2023

Biological age (BA) is a common model to evaluate the function of aging individuals as it may provide a more accurate measure of the extent of human aging than chronological age (CA). Biological age is influenced by the used biomarkers and standards in selected aging biomarkers and the statistical method to construct BA. Traditional used BA estimation approaches include multiple linear regression (MLR), principal component analysis (PCA), Klemera and Doubal’s method (KDM), and, in recent years, deep learning methods. This review summarizes the markers for each organ/system used to construct biological age and published literature using methods in BA research. Future research needs to explore the new aging markers and the standard in select markers and new methods in building BA models.
Cancer in female firefighters: The clinicobiological, psychological, and social perspectives
Authors: Kunz Kenneth Robert , Turcotte Kate , Pawer Samantha , Zheng Alex , Purewal Amanat , Wellar Alyssa , Karmali Shazya , Garis Len , Thomas Larry S. , Pike Ian
Published: April 2023

Objectives

This study explored how demographic characteristics, life experiences, and firefighting exposures have an impact on cancer among female firefighters, and described the types and biologic characteristics of cancers as reported by women in the fire service.

Methods

The online survey was available from June 2019 to July 2020. Questions related to demographic characteristics, lifestyle factors, firefighting exposures, and cancer diagnoses. Descriptive analyses characterized variables by the presence or absence of cancer. Qualitative data provided insight into both firefighting and cancer experiences among women.

Results

There were 1,344 female firefighter respondents from 12 different countries, 256 of whom provided information on their cancer diagnosis. North American respondents made up 92% of the total. Those with cancer were older, had been in the fire service longer, had more career fires and toxic exposures, and were less likely to still be in active service. They also reported more tobacco use, and more full-term pregnancies. There were no differences in family history of cancer between the two groups. The average age at diagnosis was 39.0 years. The major types of cancer reported included breast (25.4%), cervical (21.1%), melanoma (20.7%), base cell/skin (16.4%), and uterine (14.8%). The cancer was detected when seeking medical attention for symptoms (42.1%), during routine health screening (29.8%), and during specific cancer screening (28.1%). The stage of cancer was reported by 44.5%, and 30.9% included the histopathological grade. Treatments included surgery (72.7%), chemotherapy (14.8%), radiotherapy (13.7%), and observation (13.7%). Challenges associated with cancer included psychosocial (33.2%), financial (18.8%), physical (6.6%), and spiritual (6.3%). Concerns about reporting a cancer experience to their employer included the desire to keep health information private (11.3%), a feeling of vulnerability (7.4%), and being perceived as weak (7.0%). Lack of support from their employer or insurer was also noted.

Conclusion

Female firefighters experienced a wide variety of different types of cancers which may come earlier than similar cancers in the public. These findings can help inform resource allocation, the development of new policies, and the need for broader presumptive coverage to support female firefighters diagnosed with cancer.
Patterns of better breast cancer care in countries with higher human development index and healthcare expenditure: Insights from GLOBOCAN 2020
Authors: Azadnajafabad Sina , Saeedi Moghaddam Sahar , Mohammadi Esmaeil , Delazar Sina , Rashedi Sina , Baradaran Hamid Reza , Mansourian Morteza
Published: April 2023

Background

The huge burden of breast cancer (BC) necessitates the profound and accurate knowledge of the most recent cancer epidemiology and quality of care provided. We aimed to evaluate BC epidemiology and quality of care and examine the effects of socioeconomic development and healthcare expenditure on disparities in BC care.

Methods

The results from the GLOBOCAN 2020 study were utilized to extract data on female BC, including incidence and mortality numbers, crude rates, and age-standardized rates [age-standardized incidence rates (ASIRs) and age-standardized mortality rates (ASMRs)]. The mortality-to-incidence ratio (MIR) was calculated for different locations and socioeconomic stratifications to examine disparities in BC care, with higher values reflecting poor quality of care and vice versa. In both descriptive and analytic approaches, the human development index (HDI) and the proportion of current healthcare expenditure (CHE) to gross domestic product (CHE/GDP%) were used to evaluate the values of MIR.

Results

Globally, 2,261,419 (95% uncertainty interval (UI): 2,244,260–2,278,710) new cases of female BC were diagnosed in 2020, with a crude rate of 58.5/100,000 population, and caused 684,996 (675,493–694,633) deaths, with a crude rate of 17.7. The WHO region with the highest BC ASIR (69.7) was Europe, and the WHO region with the highest ASMR (19.1) was Africa. The very high HDI category had the highest BC ASIR (75.6), and low HDI areas had the highest ASMR (20.1). The overall calculated value of female BC MIR in 2020 was 0.30, with Africa having the highest value (0.48) and the low HDI category (0.53). A strong statistically significant inverse correlation was observed between the MIR and HDI values for countries/territories (Pearson's coefficient = −0.850, p-value < 0.001). A significant moderate inverse correlation was observed between the MIR and CHE/GDP values (Pearson's coefficient = −0.431, p-value < 0.001).

Conclusions

This study highlighted that MIR of BC was higher in less developed areas and less wealthy countries. MIR as an indicator of the quality of care showed that locations with higher healthcare expenditure had better BC care. More focused interventions in developing regions and in those with limited resources are needed to alleviate the burden of BC and resolve disparities in BC care.
Born too soon in a resource-limited setting: A 10-year mixed methods review of a special care baby unit for refugees and migrants on the Myanmar-Thailand border
Authors: Hashmi Ahmar , Darakamon Mu Chae , Aung Ko Ko , Mu Mu , Misa Prapatsorn , Jittamala Podjanee , Chu Cindy , Phyo Aung Pyae , Turner Claudia , Nosten Francois , McGready Rose , Carrara Verena I.
Published: April 2023

Background

Preterm birth is a major public health concern with the largest burden of morbidity and mortality falling within low- and middle-income countries (LMIC).

Materials and methods

This sequential explanatory mixed methods study was conducted in special care baby units (SCBUs) serving migrants and refugees along the Myanmar-Thailand border. It included a retrospective medical records review, qualitative interviews with mothers receiving care within SCBUs, and focus group discussions with health workers. Changes in neonatal mortality and four clinical outcomes were described. A mix of ethnographic phenomenology and implementation frameworks focused on cultural aspects, the lived experience of participants, and implementation outcomes related to SCBU care.

Results

From 2008–2017, mortality was reduced by 68% and 53% in very (EGA 28–32 weeks) and moderate (EGA 33–36 weeks) preterm neonates, respectively. Median SCBU stay was longer in very compared to moderate preterm neonates: 35 (IQR 22, 48 days) vs. 10 days (IQR 5, 16). Duration of treatments was also longer in very preterm neonates: nasogastric feeding lasted 82% (IQR 74, 89) vs. 61% (IQR 40, 76) of the stay, and oxygen therapy was used a median of 14 (IQR 7, 27) vs. 2 (IQR 1, 6) days respectively. Nine interviews were conducted with mothers currently receiving care in the SCBU and four focus group discussions with a total of 27 local SCBU staff. Analysis corroborated quantitative analysis of newborn care services in this setting and incorporated pertinent implementation constructs including coverage, acceptability, appropriateness, feasibility, and fidelity. Coverage, acceptability, and appropriateness were often overlapping outcomes of interest highlighting financial issues prior to or while admitted to the SCBU and social issues and support systems adversely impacting SCBU stays. Interview and FGD findings highlight the barriers in this resource-limited setting as they impact the feasibility and fidelity of providing evidence-based SCBU care that often required adaptation to fit the financial and environmental constraints imposed by this setting.

Discussion

This study provides an in-depth look at the nature of providing preterm neonatal interventions in a SCBU for a vulnerable population in a resource-limited setting. These findings support implementation of basic evidence-based interventions for preterm and newborn care globally, particularly in LMICs.
The COVID-19 pandemic and Bitcoin: Perspective from investor attention
Authors: Wan Jieru , Wu You , Zhu Panpan
Published: April 2023

The response of the Bitcoin market to the novel coronavirus (COVID-19) pandemic is an example of how a global public health crisis can cause drastic market adjustments or even a market crash. Investor attention on the COVID-19 pandemic is likely to play an important role in this response. Focusing on the Bitcoin futures market, this paper aims to investigate whether pandemic attention can explain and forecast the returns and volatility of Bitcoin futures. Using the daily Google search volume index for the “coronavirus” keyword from January 2020 to February 2022 to represent pandemic attention, this paper implements the Granger causality test, Vector Autoregression (VAR) analysis, and several linear effects analyses. The findings suggest that pandemic attention is a granger cause of Bitcoin returns and volatility. It appears that an increase in pandemic attention results in lower returns and excessive volatility in the Bitcoin futures market, even after taking into account the interactive effects and the influence of controlling other financial markets. In addition, this paper carries out the out-of-sample forecasts and finds that the predictive models with pandemic attention do improve the out-of-sample forecast performance, which is enhanced in the prediction of Bitcoin returns while diminished in the prediction of Bitcoin volatility as the forecast horizon is extended. Finally, the predictive models including pandemic attention can generate significant economic benefits by constructing portfolios among Bitcoin futures and risk-free assets. All the results demonstrate that pandemic attention plays an important and non-negligible role in the Bitcoin futures market. This paper can provide enlightens for subsequent research on Bitcoin based on investor attention sparked by public emergencies.
Active aging awareness and well-being among older adults in Portugal
Authors: Costa Andreia , Henriques Joana , Alarcão Violeta , Henriques Adriana , Madeira Teresa , Virgolino Ana , Sousa Joana , Feteira-Santos Rodrigo , Arriaga Miguel , Rocha Jorge , Nogueira Paulo
Published: April 2023

Objective

This study aims to assess the active aging awareness of older adults in mainland Portugal and their levels of overall well-being and to identify social and health-related factors.

Methods

A cross-sectional study was conducted with a representative sample of 613 older adults, aged 65 or older, who participated in the PROKnos – Knowing Social Prescribing needs of the elderly study in Portugal. The questionnaire consisted of the Active Ageing Awareness Questionnaire and the World Health Organization – Five Well-Being Index, as well as sociodemographic, economic, and health status questions. Correlation coefficients, t-tests for independent samples, and one-way ANOVA were used to explore potential associations between variables.

Results

The active aging awareness levels were significantly higher for women (p = 0.031), and those who were younger (p = 0.011), more educated (p < 0.001), had a better financial situation (p < 0.001), and had better health (p < 0.001). The same pattern was found for well-being, except in relation to gender, as men had higher levels (p = 0.016). These variables were found to be correlated.

Discussion

Even though active aging is an important strategy to implement, it is indispensable to consider the perceptions and conditions that need to be in place before that. This study reveals that several social and health-related factors are associated with well-being and active aging awareness, as well as the differences between groups that exist in mainland Portugal in relation to that. This emphasizes how vital it is to address social inequalities in active aging efforts, which are not necessarily uncovered when only considering actual active aging measures.
Veterans Health Administration response to 2021 recall of Philips Respironics devices: A case study
Authors: Belkora Jeffrey K. , Fields Barry , Shamim-Uzzaman Q. Afifa , Stratford Donna , Alfandre David , Hollingshaus Scott , Yackel Edward , Geppert Cynthia , Nechanicky Penny , Nichols Ardene , Williams Katherine , Reichert Jill , Whooley Mary A. , Francis Joe , Sarmiento Kathleen F.
Published: April 2023

This case study describes, for the time frame of June 2021 through August 2022, the U.S. Veterans Health Administration (VHA) organizational response to a manufacturer's recall of positive airway pressure devices used in the treatment of sleep disordered breathing. VHA estimated it could take over a year for Veterans to receive replacement devices. Veterans awaiting a replacement faced a dilemma. They could continue using the recalled devices and bear the product safety risks that led to the recall, or they could stop using them and bear the risks of untreated sleep disordered breathing. Using a program monitoring approach, we report on the processes VHA put in place to respond to the recall. Specifically, we report on the strategic, service, and operational plans associated with VHA's response to the recall for Veterans needing replacement devices. In program monitoring, the strategic plan reflects the internal process objectives for the program. The service plan articulates how the delivery of services will intersect the customer journey. The operational plan describes how the program's resources and actions must support the service delivery plan. VHA's strategic plan featured a clinician-led, as opposed to primarily legal or administrative response to the recall. The recall response team also engaged with VHA's medical ethics service to articulate an ethical framework guiding the allocation of replacement devices under conditions of scarcity. This framework proposed allocating scarce devices to Veterans according to their clinical need. The service plan invited Veterans to schedule visits with sleep providers who could assess their clinical need and counsel them accordingly. The operational plan distributed devices according to clinical need as they became available. Monitoring our program processes in real time helped VHA launch and adapt its response to a recall affecting more than 700,000 Veterans.
Perception and use of flywheel resistance training amongst therapists in sport
Authors: de Keijzer Kevin L. , Raya-González Javier , López Samanés Álvaro , Moreno Perez Victor , Beato Marco
Published: April 2023

Flywheel (isoinertial) resistance training is a valid strength training method that has been incorporated in sport for decades, yet little is known about how therapists working in sport apply flywheel resistance training. We aimed to describe and understand current application and perception of flywheel resistance training amongst therapists working in sport. Seventy- three therapists (13 ± 10 years of experience) started part of this survey with 52 completing the entire electronic questionnaire. Nine multiple choice questions on application and perceptions of flywheel training (prerequisites, use of technology, barriers, and upper- and lower-body exercises) preceded two 6-point Likert scale statements on strength and reduction of injury likelihood. Most therapists (47/73) either used or intended to use flywheel training with their athletes and stated familiarisation would be a priority prior to initiating training. Although more than half suggested they were confident flywheel training could enhance strength (27/52) and muscular prehabilitation outcomes (40/52), many remained unsure. Nonetheless, it appears that therapists would mostly include flywheel training within prehabilitation (40/52) or during the later stages of rehabilitation (37/52). To monitor progress, therapists slightly prefer power (30/52) over velocity outputs, while few would not use them at all. Although therapists would prescribe most exercises - the squat, rotational exercise, and unilateral leg curl would be the most selected. Meanwhile, therapists reported remain most unsure or would avoid prescribing the lateral squat and unilateral hip extension. The biggest perceived barriers to flywheel training are equipment cost/space, evidence, and scheduling. The investigation provides valuable insight into the application and perception of flywheel training amongst therapists working in sport.
Sustainable beef labeling in Latin America and the Caribbean: Initiatives, developments, and bottlenecks
Authors: Lerma Leonardo Moreno , Díaz Baca Manuel Francisco , Burkart Stefan
Published: April 2023

Scientific research is increasingly conclusive regarding the responsibility of food production in environmental issues, a situation that contrasts with greater consumer awareness. In this context, sustainability labels for meat have emerged, which offer a guarantee that production is based on principles of animal welfare, and carbon neutrality, among others. Since research on this subject is scarce, the objective of this article is to identify and analyze the initiatives and development of this type of labeling in Latin America and the Caribbean. For this purpose, a qualitative-descriptive study is carried out based on primary and secondary sources. The results show different types of progress in the main producing countries, with Uruguay and Brazil standing out as success stories. At the same time, difficulties are highlighted in the different stages of the production chain, such as low demand, or traceability. It is concluded that, despite the challenges, the transition to sustainable cattle farming is inevitable. However, this process will not happen spontaneously, but must be coordinated up with other types of strategies and the actions of various actors including governments, policymakers, and NGOs.
Intra-osseous plasma rich in growth factors enhances cartilage and subchondral bone regeneration in rabbits with acute full thickness chondral defects: Histological assessment
Authors: Torres-Torrillas Marta , Damia Elena , Romero Ayla del , Pelaez Pau , Miguel-Pastor Laura , Chicharro Deborah , Carrillo José M. , Rubio Mónica , Sopena Joaquín J.
Published: April 2023

Background

Intra-articular (IA) combined with intra-osseous (IO) infiltration of plasma rich in growth factors (PRGF) have been proposed as an alternative approach to treat patients with severe osteoarthritis (OA) and subchondral bone damage. The aim of the study is to evaluate the efficacy of IO injections of PRGF to treat acute full depth chondral lesion in a rabbit model by using two histological validated scales (OARSI and ICRS II).

Methodology

A total of 40 rabbits were included in the study. A full depth chondral defect was created in the medial femoral condyle and then animals were divided into 2 groups depending on the IO treatment injected on surgery day: control group (IA injection of PRGF and IO injection of saline) and treatment group (IA combined with IO injection of PRGF). Animals were euthanized 56 and 84 days after surgery and the condyles were processed for posterior histological evaluation.

Results

Better scores were obtained in treatment group in both scoring systems at 56- and 84-days follow-up than in control group. Additionally, longer-term histological benefits have been obtained in the treatment group.

Conclusions

The results suggests that IO infiltration of PRGF enhances cartilage and subchondral bone healing more than the IA-only PRGF infiltration and provides longer-lasting beneficial effects.
A Double-Layer Model Predictive Control Approach for Collision-Free Lane Tracking of On-Road Autonomous Vehicles
Authors: Yang Weishan , Chen Yuepeng , Su Yixin
Published: April 2023

This paper proposes a double-layer model predictive control (MPC) algorithm for the integrated path planning and trajectory tracking of autonomous vehicles on roads. The upper module is responsible for generating collision-free lane trajectories, while the lower module is responsible for tracking this trajectory. A simplified vehicle model based on the friction cone is proposed to reduce the computation time for trajectory planning in the upper layer module. To achieve dynamic and accurate collision avoidance, a polygonal distance-based dynamic obstacle avoidance method is proposed. A vertical load calculation method for the tires is introduced to design the anti-rollover constraint in the lower layer module. Numerical simulations, with static and dynamic obstacle scenarios, are conducted on the MATLAB platform and compared with two state-of-the-art MPC algorithms. The results demonstrate that the proposed algorithm outperforms the other two algorithms regarding computation time and collision avoidance efficiency.
Automatic Aluminum Alloy Surface Grinding Trajectory Planning of Industrial Robot Based on Weld Seam Recognition and Positioning
Authors: Zhao Hong , Wen Ke , Lei Tianjian , Xiao Yinan , Pan Yang
Published: April 2023

In this paper, we propose a novel method for planning grinding trajectories on curved surfaces to improve the grinding efficiency of large aluminum alloy surfaces with welds and defect areas. Our method consists of three parts. Firstly, we introduce a deficiency positioning method based on a two-dimensional image and three-dimensional point cloud, which enables us to accurately and quickly locate the three-dimensional defective areas. Secondly, we propose a 2D weld positioning method based on the defect area and obtain the spatial position of the 3D weld by combining the relationship between 2D and 3D images. Additionally, we propose an orthogonal projection method from the point cloud to the aluminum alloy surface to calculate the weld reinforcement. Thirdly, we present a space spiral grinding trajectory planning method for complex curved surfaces based on the characteristics of the weld reinforcement, spatial position, and spatial position information of the defect area. This method shortens the grinding time of the defect area and improves efficiency. Simulation and experimental results show that our grinding trajectory planning method is more efficient than other grinding methods in removing defects from the surface of aluminum alloys. Moreover, the defect area after grinding is smoother than before.
The Effects of Trichoderma viride T23 on Rhizosphere Soil Microbial Communities and the Metabolomics of Muskmelon under Continuous Cropping
Authors: Zhang Zhaoran , Tang Shuangshuang , Liu Xiaodi , Ren Xuelian , Wang Suna , Gao Zenggui
Published: April 2023

The continuous cropping can restrict the large scale and intensive cultivation of muskmelon, and the use of Trichoderma preparation to alleviate the negative effects is an effective mean. Although the impact on rhizosphere soil microbial communities and metabolites after applying Trichoderma are still unclear. In this study, we applied the fermentation broth of Trichoderma viride T23 to muskmelon under continuous cropping, collected rhizosphere soil samples at 60 days after transplantation, and investigated the changes in the microbial communities and metabolites of muskmelon by using high−throughput sequencing and metabolomic analysis, respectively. The results showed that T. viride T23 could effectively reduce the disease index of muskmelon wilt (65.86 to 18) and significantly increase the soil pH value (6.06 to 6.40). Trichoderma viride T23 induced drastic shifts in the richness, structure, and composition of rhizosphere microbial communities, and Proteobacteria, Bacteroidetes, and Actinobacteria were the dominant bacterial phyla. Bioactive substances such as scopoletin, erythronic acid, and palmitic acid were significantly upregulated in the rhizosphere soil, which enhanced soil activity. Overall, T. viride T23 resolves the continuous cropping limitation in muskmelon by improving soil physicochemical properties, elevating the biomass and diversity of soil microbial communities, and stimulating the production of soil active substances.
Do System of Rice Intensification Practices Produce Rice Plants Phenotypically and Physiologically Superior to Conventional Practice?
Authors: Thakur Amod Kumar , Mandal Krishna Gopal , Verma Om Prakash , Mohanty Rajeeb Kumar
Published: April 2023

The System of Rice Intensification (SRI), an agro-ecological approach to rice cultivation developed in Madagascar, has generated considerable interest worldwide. Having not been developed at a research establishment but rather from observation and testing on farmers’ fields, SRI attracted considerable controversy, for example, with unwarranted objections that it lacked of scientific evidence, and being characterized as based on ‘unconfirmed field observations’ (UFOs). One 2004 article concluded that “the system of rice intensification does not fundamentally change the physiological yield potential of rice”. This assertion was not based on any physiological examination of rice plants grown using SRI methodology, however, or on any systematic comparison with what would be considered as best management practices (BMPs), recommended practices (RPs), or farmer practices (FPs). Other dismissals of SRI have had contestable data selection, analytical methods, and presentation of results. The published literature provides abundant evidence that the earlier evaluations of SRI were either not well-informed or objective, and possibly, they discouraged others from embarking on systematic evaluations of their own. This article examines the results of 78 studies in the published literature where SRI results were explicitly compared with RPs, including BMPs or FPs. The results from 27 countries, plus several large-scale evaluations, show that in 80% of the evaluations, grain yield was higher under SRI than with RPs or FPs. SRI gave 24% higher grain yield than RPs and 56% more than FPs, while reducing seed, water, and fertilizer inputs. Beyond the descriptive evidence, this paper considers that the phenotypical changes and physiological improvements in SRI-grown rice plants could account for the reported enhancement in yield. More research remains to be undertaken to elucidate casual mechanisms, but abundant evidence shows that this is a subject deserving considerable scientific effort.
Post-Stroke Infections: Insights from Big Data Using Clinical Data Warehouse (CDW)
Authors: Jung Moa , Park Hae-Yeon , Park Geun-Young , Lee Jong In , Kim Youngkook , Kim Yeo Hyung , Lim Seong Hoon , Yoo Yeun Jie , Im Sun
Published: April 2023

This study analyzed a digitized database of electronic medical records (EMRs) to identify risk factors for post-stroke infections. The sample included 41,236 patients hospitalized with a first stroke diagnosis (ICD-10 codes I60, I61, I63, and I64) between January 2011 and December 2020. Logistic regression analysis was performed to examine the effect of clinical variables on post-stroke infection. Multivariable analysis revealed that post-stroke infection was associated with the male sex (odds ratio [OR]: 1.79; 95% confidence interval [CI]: 1.49–2.15), brain surgery (OR: 7.89; 95% CI: 6.27–9.92), mechanical ventilation (OR: 18.26; 95% CI: 8.49–44.32), enteral tube feeding (OR: 3.65; 95% CI: 2.98–4.47), and functional activity level (modified Barthel index: OR: 0.98; 95% CI: 0.98–0.98). In addition, exposure to steroids (OR: 2.22; 95% CI: 1.60–3.06) and acid-suppressant drugs (OR: 1.44; 95% CI: 1.15–1.81) increased the risk of infection. On the basis of the findings from this multicenter study, it is crucial to carefully evaluate the balance between the potential benefits of acid-suppressant drugs or corticosteroids and the increased risk of infection in patients at high risk for post-stroke infection.
The Impact of Drugs on Hydrogen Sulfide Homeostasis in Mammals
Authors: Alsaeedi Asrar , Welham Simon , Rose Peter , Zhu Yi-Zhun
Published: April 2023

Mammalian cells and tissues have the capacity to generate hydrogen sulfide gas (H2S) via catabolic routes involving cysteine metabolism. H2S acts on cell signaling cascades that are necessary in many biochemical and physiological roles important in the heart, brain, liver, kidney, urogenital tract, and cardiovascular and immune systems of mammals. Diminished levels of this molecule are observed in several pathophysiological conditions including heart disease, diabetes, obesity, and immune function. Interestingly, in the last two decades, it has become apparent that some commonly prescribed pharmacological drugs can impact the expression and activities of enzymes responsible for hydrogen sulfide production in cells and tissues. Therefore, the current review provides an overview of the studies that catalogue key drugs and their impact on hydrogen sulfide production in mammals.
Evaluation of the Effect of Photodynamic Therapy on CAM-Grown Sarcomas
Authors: Kerkhoff Maximilian , Grunewald Susanne , Schaefer Christiane , Zöllner Stefan K. , Plaumann Pauline , Busch Maike , Dünker Nicole , Ketzer Julia , Kersting Josephine , Bauer Sebastian , Hardes Jendrik , Streitbürger Arne , Dirksen Uta , Hartmann Wolfgang , Guder Wiebke K.
Published: April 2023

Resection margin adequacy plays a critical role in the local control of sarcomas. Fluorescence-guided surgery has increased complete resection rates and local recurrence-free survival in several oncological disciplines. The purpose of this study was to determine whether sarcomas exhibit sufficient tumor fluorescence (photodynamic diagnosis (PDD)) after administration of 5-aminolevulinic acid (5-ALA) and whether photodynamic therapy (PDT) has an impact on tumor vitality in vivo. Sixteen primary cell cultures were derived from patient samples of 12 different sarcoma subtypes and transplanted onto the chorio-allantoic membrane (CAM) of chick embryos to generate 3-dimensional cell-derived xenografts (CDXs). After treatment with 5-ALA, the CDXs were incubated for another 4 h. Subsequently accumulated protoporphyrin IX (PPIX) was excited by blue light and the intensity of tumor fluorescence was analyzed. A subset of CDXs was exposed to red light and morphological changes of both CAMs and tumors were documented. Twenty-four hours after PDT, the tumors were excised and examined histologically. High rates of cell-derived engraftments on the CAM were achieved in all sarcoma subtypes and an intense PPIX fluorescence was observed. PDT of CDXs resulted in a disruption of tumor-feeding vessels and 52.4% of CDXs presented as regressive after PDT treatment, whereas control CDXs remained vital in all cases. Therefore, 5-ALA mediated PDD and PDT appear to be promising tools in defining sarcoma resection margins (PDD) and adjuvant treatment of the tumor bed (PDT).
Long-Term Culture Performance of a Polyelectrolyte Complex Microcapsule Platform for Hyaline Cartilage Repair
Authors: Arhebamen Ehinor P. , Teodoro Maria T. , Blonka Amelia B. , Matthew Howard W. T.
Published: April 2023

Articular cartilage (AC) tissue repair and regeneration remains an ongoing challenge. One component of the challenge is the limited ability to scale an engineered cartilage graft to clinically relevant sizes while maintaining uniform properties. In this paper, we report on the evaluation of our polyelectrolyte complex microcapsule (PECM) platform technology as a technique for generating cartilage-like spherical modules. Bone marrow-derived mesenchymal stem cells (bMSCs) or primary articular chondrocytes were encapsulated within PECMs composed of methacrylated hyaluronan, collagen I, and chitosan. The formation of cartilage-like tissue in the PECMs over a 90-day culture was characterized. The results showed that chondrocytes exhibited superior growth and matrix deposition compared to either chondrogenically-induced bMSCs or a mixed PECM culture containing both chondrocytes and bMSCs. The chondrocyte-generated matrix filled the PECM and produced substantial increases in capsule compressive strength. The PECM system thus appears to support intracapsular cartilage tissue formation and the capsule approach promotes efficient culture and handling of these micro tissues. Since previous studies have proven the feasibility of fusing such capsules into large tissue constructs, the results suggest that encapsulating primary chondrocytes in PECM modules may be a viable route toward achieving a functional articular cartilage graft.
Establishment of Surgical Difficulty Grading System and Application of MRI-Based Artificial Intelligence to Stratify Difficulty in Laparoscopic Rectal Surgery
Authors: Sun Zhen , Hou Wenyun , Liu Weimin , Liu Jingjuan , Li Kexuan , Wu Bin , Lin Guole , Xue Huadan , Pan Junjun , Xiao Yi
Published: April 2023

(1) Background: The difficulty of pelvic operation is greatly affected by anatomical constraints. Defining this difficulty and assessing it based on conventional methods has some limitations. Artificial intelligence (AI) has enabled rapid advances in surgery, but its role in assessing the difficulty of laparoscopic rectal surgery is unclear. This study aimed to establish a difficulty grading system to assess the difficulty of laparoscopic rectal surgery, as well as utilize this system to evaluate the reliability of pelvis-induced difficulties described by MRI-based AI. (2) Methods: Patients who underwent laparoscopic rectal surgery from March 2019 to October 2022 were included, and were divided into a non-difficult group and difficult group. This study was divided into two stages. In the first stage, a difficulty grading system was developed and proposed to assess the surgical difficulty caused by the pelvis. In the second stage, AI was used to build a model, and the ability of the model to stratify the difficulty of surgery was evaluated at this stage, based on the results of the first stage; (3) Results: Among the 108 enrolled patients, 53 patients (49.1%) were in the difficult group. Compared to the non-difficult group, there were longer operation times, more blood loss, higher rates of anastomotic leaks, and poorer specimen quality in the difficult group. In the second stage, after training and testing, the average accuracy of the four-fold cross validation models on the test set was 0.830, and the accuracy of the merged AI model was 0.800, the precision was 0.786, the specificity was 0.750, the recall was 0.846, the F1-score was 0.815, the area under the receiver operating curve was 0.78 and the average precision was 0.69; (4) Conclusions: This study successfully proposed a feasible grading system for surgery difficulty and developed a predictive model with reasonable accuracy using AI, which can assist surgeons in determining surgical difficulty and in choosing the optimal surgical approach for rectal cancer patients with a structurally difficult pelvis.