bluuebunny/crossref_metadata_2025 huggingface dataset:
Dental sectioning for intraoral equine cheek teeth extractions: 29 cases
Authors: Leps Alexis , Korsos Szabolcs , Clarysse Michèle , Vlaminck Lieven
Published: February 2024

The objectives of this retrospective study were to describe cheek teeth extraction by the sectioning technique, the decision making to use this technique and its potentially associated complications. Sectioning for dental extraction purpose was used in 29/461 (6.3%) of cases. Oro-sinusal fistula was the main post-operative complication, with 4/29 (13.7%) cases developing a macroscopic communication between the alveolus of the tooth extracted and the adjacent sinus compartment. All teeth where sectioning was attempted were successfully extracted. Sectioning for dental extraction appears to be a safe technique that can be used instead of or in addition too other minimal invasive cheek teeth extraction techniques. Thorough preoperative planning including oroscopic examination and medical imaging modalities are required to help in decision making, as well as excellent sedation and analgesia and horse compliance.
Enterovirus-D68 – a reemerging non-polio enterovirus that causes severe respiratory and neurological disease in children
Authors: Grizer Cassandra S. , Messacar Kevin , Mattapallil Joseph J.
Published: February 2024

The past decade has seen the global reemergence and rapid spread of enterovirus D68 (EV-D68), a respiratory pathogen that causes severe respiratory illness and paralysis in children. EV-D68 was first isolated in 1962 from children with pneumonia. Sporadic cases and small outbreaks have been reported since then with a major respiratory disease outbreak in 2014 associated with an increased number of children diagnosed with polio-like paralysis. From 2014-2018, major outbreaks were reported every other year in a biennial pattern with > 90% of the cases occurring in children under the age of 16. With the outbreak of SARS-CoV-2 and the subsequent COVID-19 pandemic, there was a significant decrease in the prevalence EV-D68 cases along with other respiratory diseases. However, since the relaxation of pandemic social distancing protocols and masking mandates the number of EV-D68 cases have begun to rise again-culminating in another outbreak in 2022. Here we review the virology, pathogenesis, and the immune response to EV-D68, and discuss the epidemiology of EV-D68 infections and the divergence of contemporary strains from historical strains. Finally, we highlight some of the key challenges in the field that remain to be addressed.
Global Surface Pressure Pattern for a Compressible Elliptical Cavity Flow Using Pressure-Sensitive Paint
Authors: Huang Yi-Xuan , Chung Kung-Ming
Published: February 2024

The flow field in a cavity depends on the properties of the upstream boundary layer and the cavity geometry. Comprehensive studies for rectangular cavities have been conducted. This experimental study determines the global surface pressure pattern for elliptical cavities (eccentricities of 0, 0.66 and 0.87) in a naturally developed turbulent boundary layer using pressure-sensitive paint. The ratio between the length (major axis) and the depth is 4.43–21.5, and the freestream Mach number is 0.83. The mean surface pressure distribution of an elliptical cavity resembles that of a rectangular cavity. A change in the value of eccentricity (wall curvature) affects the region for an adverse pressure gradient in an open cavity, an extension of the plateau in a transitional–closed cavity and flow expansion near the front and rear edges. The boundaries between an open, transitional and closed cavities vary.
Theoretical Study of the Motion of a Cut Sugar Beet Tops Particle along the Inner Surface of the Conveying and Unloading System of a Topping Machine
Authors: Pascuzzi Simone , Bulgakov Volodymyr , Holovach Ivan , Ivanovs Semjons , Aboltins Aivars , Ihnatiev Yevhen , Rucins Adolfs , Trokhaniak Oleksandra , Paciolla Francesco
Published: February 2024

One of the most delicate operations in the sugar beet harvesting process is removing the tops from the heads of the root crops without any mechanical damages. The aim of this study is to improve the design of the conveying and unloading system of the sugar beet topper machine. In this paper, a mathematical model of the motion of a cut beet tops particle M, along the conveying and unloading system, has been developed to support the evaluation of kinematic and design parameters, depending on the rotational speed of the thrower blade, the air flow speed, the required ejection speed of particle M, and the position of the trailer that moves alongside the harvester. It has been established that increasing the speed Va of the top particle M, which has left the end of the blade of the thrower, leads to an increase in the arc coordinate S(t) of its movement along the cylindrical section of the casing. Within the range of the speed change from 4 m·s–1 to 8 m·s–1, the value of the arc coordinate S(t) increases by 1.4 times during time t = 0.006 s. Moreover, a rapid decrease in speed V is observed with an increase in the length x of the discharge chute.
Remediation of Cadmium and Lead in Mine Soil by Ameliorants and Its Impact on Maize (Zea mays L.) Cultivation
Authors: Chen Qiyue , Wang Lei , Li Bo , He Siteng , Li Yang , He Yongmei , Liang Xinran , Zhan Fangdong
Published: February 2024

The soil in a lead–zinc mining area, contaminated with heavy metals like cadmium (Cd) and lead (Pb), poses a risk to crops such as maize. Experiments using biochar and sepiolite as soil ameliorants in potted maize showed these substances can mitigate heavy metal contamination. Biochar increased potassium and phosphorus in the soil and maize, while sepiolite significantly boosted overground phosphorus by 73.2%. Both ameliorants transformed Cd and Pb into a more stable state in the soil, reducing their accumulation in maize, especially with biochar, which effectively inhibited metal migration during leaching events. This study provided insights for further improvement of soil amendments and multi-factor application experiments.
The Impact of Patient-Specific Positions on the Relationship between Iliac Blood Vessels and Lumbar Intervertebral Discs: Anatomical Significance and Clinical Implications
Authors: Bečulić Hakija , Begagić Emir , Džidić-Krivić Amina , Pugonja Ragib , Jaganjac Belma , Imamović-Bošnjak Melica , Selimović Edin , Čeliković Amila , Skomorac Rasim , Efendić Alma , Alić Fahrudin , Mašović Anes , Terzić-Salihbašić Selma , Tandir-Lihić Lejla , Pojskić Mirza
Published: February 2024

This study explores the anatomical relationship between iliac blood vessels and the lower lumbar spine during supine and prone patient positions. The average height of participants was 174.02 cm ± 9.01, while the average weight was 80.38 kg ± 13.48. Body mass index (BMI) analyses showed differences (p = 0.002), with 34.7% classified as normal weight, 53.1% as overweight, and 12.2% as moderately obese. The study examined the distances between iliac arteries and veins in relation to intervertebral anterior and posterior disc contours. Patient positioning significantly affected these measurements at both L4/L5 and L5/S1 levels. The findings highlight the critical influence of body position on anatomical relationships in the context of lower lumbar spine surgery. The study underscores the importance of preoperative awareness of vascular anatomy to prevent iatrogenic lesions during spine surgery, contributing valuable insights for optimizing surgical approaches and minimizing complications in spine surgery, particularly microdiscectomy.
Patterns of Wolf Dispersal Respond to Harvest Density across an Island Complex
Authors: Roffler Gretchen H. , Pilgrim Kristine L. , Williams Benjamin C.
Published: February 2024

Wolves are highly mobile predators and can disperse across a variety of habitats and over long distances. However, less is known about dispersal capabilities across water and among islands. The biogeography of island systems fosters spatially structured local populations, and their degree of connectivity may influence the dynamics and long-term viability of the regional population. We sought to quantify wolf dispersal rate, distance, and dispersal sex bias throughout Prince of Wales Island, a 6670 km2 island in southeast Alaska, and the surrounding islands that constitute the wildlife management unit (9025 km2). We also investigated patterns of dispersal in relation to hunting and trapping intensity and wolf population density. We used DNA data collected during 2012–2021 long-term monitoring efforts and genotyped 811 wolves, 144 of which (18%) were dispersers. Annual dispersal rates were 9–23% and had a weakly positive relationship with wolf density. Wolves dispersed 41.9 km on average (SD = 23.7 km), and males and females did not disperse at different rates. Of the dispersing wolves, 107 died, and the majority (n = 81) died before they were able to settle. The leading manner of death was trapping (97% of mortalities), and wolves tended to disperse from areas with low harvest density to areas where harvest density was relatively higher. Dispersal occurred both to and from small islands and the larger Prince of Wales Island, indicating bidirectional as opposed to asymmetrical movement, and the genetic overlap of wolf groups demonstrates connectivity throughout this naturally patchy system. Island ecosystems have different predator–prey dynamics and recolonization processes than large, intact systems due to their isolation and restricted sizes; thus, a better understanding of the degree of population connectivity including dispersal patterns among islands in the Prince of Wales archipelago could help inform the management and research strategies of these wolves.
Catastrophic Streptococcus pyogenes Disease: A Personalized Approach Based on Phenotypes and Treatable Traits
Authors: Ruiz-Rodríguez Juan Carlos , Chiscano-Camón Luis , Maldonado Carolina , Ruiz-Sanmartin Adolf , Martin Laura , Bajaña Ivan , Bastidas Juliana , Lopez-Martinez Rocio , Franco-Jarava Clara , González-López Juan José , Ribas Vicent , Larrosa Nieves , Riera Jordi , Nuvials-Casals Xavier , Ferrer Ricard
Published: February 2024

Streptococcal toxic shock syndrome (STTS) is a critical medical emergency marked by high morbidity and mortality, necessitating swift awareness, targeted treatment, and early source control due to its rapid symptom manifestation. This report focuses on a cohort of 13 patients admitted to Vall d’Hebron University Hospital Intensive Care Unit, Barcelona, from November 2022 to March 2023, exhibiting invasive Streptococcus pyogenes infections and meeting institutional sepsis code activation criteria. The primary infections were community-acquired pneumonia (61.5%) and skin/soft tissue infection (30.8%). All patients received prompt antibiotic treatment, with clinical source control through thoracic drainage (30.8%) or surgical means (23.1%). Organ support involved invasive mechanical ventilation, vasopressors, and continuous renal replacement therapy as per guidelines. Of note, 76.9% of patients experienced septic cardiomyopathy, and 53.8% required extracorporeal membrane oxygenation (ECMO). The study identified three distinct phenotypic profiles—hyperinflammatory, low perfusion, and hypogammaglobulinemic—which could guide personalized therapeutic approaches. STTS, with a mean SOFA score of 17 (5.7) and a 53.8% requiring ECMO, underscores the need for precision medicine-based rescue therapies and sepsis phenotype identification. Integrating these strategies with prompt antibiotics and efficient source control offers a potential avenue to mitigate organ failure, enhancing patient survival and recovery in the face of this severe clinical condition.
Amelioration of Functional, Metabolic, and Morphological Deterioration in the Retina following Retinal Detachment by Green Tea Extract
Authors: Chu Kai On , Yip Yolanda Wong Ying , Chan Kwok Ping , Wang Chi Chiu , Ng Danny Siu Chun , Pang Chi Pui
Published: February 2024

Retinal detachment (RD) can result in the loss of photoreceptors that cause vision impairment and potential blindness. This study explores the protective effects of the oral administration of green tea extract (GTE) in a rat model of RD. Various doses of GTE or epigallocatechin gallate (EGCG), the most active ingredient in green tea catechins, were administered to Sprague Dawley (SD) rats with experimentally induced retinal detachment. The rats received sub-retinal injections of hyaluronic acid (0.1%) to induce RD and were given different doses of GTE and EGCG twice daily for three days. Notably, a low dose of GTE (142.9 mg/kg) caused significantly higher signal amplitudes in electroretinograms (ERGs) compared to higher GTE doses and any doses of EGCG. After administration of a low dose of GTE, the outer nuclear layer thickness, following normalization, of the detached retina reduced to 82.4 ± 8.2% (Mean ± SEM, p < 0.05) of the thickness by RD treatment. This thickness was similar to non-RD conditions, at 83.5 ± 4.7% (Mean ± SEM) of the thickness following RD treatment. In addition, the number of TUNEL-positive cells decreased from 76.7 ± 7.4 to 4.7 ± 1.02 (Mean ± SEM, p < 0.0001). This reduction was associated with the inhibition of apoptosis through decreased sphingomyelin levels and mitigation of oxidative stress shown by a lowered protein carbonyl level, which may involve suppression of HIF-1α pathways. Furthermore, GTE showed anti-inflammatory effects by reducing inflammatory cytokines and increasing resolving cytokines. In conclusion, low-dose GTE, but not EGCG, significantly alleviated RD-induced apoptosis, oxidative stress, inflammation, and energy insufficiency within a short period and without affecting energy metabolism. These findings suggest the potential of low-dose GTE as a protective agent for the retina in RD.
Dynamic Modeling, Dynamic Characteristics, and Slight Self-Guidance Ability at High Speeds of Independently Rotating Wheelset for Railway Vehicles
Authors: Jia Xingheng , Yin Yuming , Wang Wenjun
Published: February 2024

For better small-radius curve negotiation performance, the independently rotating wheelset has the potential to be equipped in urban rail transit. As a crucial part of the running gear, its dynamic characteristics directly affect the railway vehicle’s stability and curve negotiation ability. This study follows a model–simulation–experiment method to delve into the dynamic process and steady convergent process of the independently rotating wheelset. An improved mathematical dynamic model of the independently rotating wheelset is established, considering the gravitational restoring forces of the wheelset and different creepages between the left and right wheels. In addition, the gyroscopic effects on the independently rotating wheelset with positive wheel tread conicity and at high speeds are introduced and analyzed. With variations in the longitudinal speed and yaw suspension coefficients, three kinds of motions, derailment, hunting, and offset running, occur on the independently rotating wheelset. We find that the gyroscopic effects contribute to the slight self-guidance ability of the independently rotating wheelset, causing a hunting motion at high speeds. Through sufficient simulations, the improved mathematical dynamic model is verified to be closer to the dynamic model built in the general multibody system simulation software SIMPACK 2018 than the classical mathematical dynamic model. Further, we perform experiments on a scaled independently rotating wheelset experiment system. The dynamic characteristics derived from theoretical analysis, especially the slight self-guidance ability at high speeds, are verified.
Ranking of Technologies for Intralogistic Bulk Material Handling Processes Using Fuzzy Step-Wise Weight Assessment Ratio Analysis and Axial-Distance-Based Aggregated Measurement Methods
Authors: Tadić Snežana , Krstić Mladen , Božić Mladen , Dabić-Miletić Svetlana , Zečević Slobodan
Published: February 2024

The logistics network is considered the provider of logistics activities in supply chains. The fluctuating requirements of customers and the logistics network’s complex structure are only a few of the factors that cause challenges to its management. Industrial facilities are particularly vulnerable to challenges because material handling operations dominate in addition to manufacturing activities. Disruptions at industrial plants are disseminated through the logistics network, affecting all supply chain participants. As a result, reducing material handling time and costs to decrease material losses, pollution, and productivity is vital to their business. Due to their distinctive properties and significant share in finished goods, bulk materials are particularly vulnerable to issues during manufacturing. Accordingly, this study aims to rank and select technologies for handling bulk materials in an industrial plant where the production of construction materials is performed. This paper proposes four alternative solutions for the observed case study, and nine criteria were selected for the evaluation. A new hybrid multi-criteria decision-making model is proposed. The model combines Fuzzy Step-Wise Weight Assessment Ratio Analysis (SWARA), used to determine the weight of criteria, and the Axial-Distance-Based Aggregated Measurement (ADAM) method, used to rank alternative solutions. The model results indicate that the pneumatic conveyor is the best ranked alternative that significantly increases productivity, reduces losses, and improves working conditions. The key contributions of this study are its analysis of the efficiency of the technologies proposed for bulk material handling and the development and implementation of a model framework for the ranking of these technologies.
Centralized Database Access: Transformer Framework and LLM/Chatbot Integration-Based Hybrid Model
Authors: Bratić Diana , Šapina Marko , Jurečić Denis , Žiljak Gršić Jana
Published: February 2024

This paper addresses the challenges associated with the centralized storage of educational materials in the context of a fragmented and disparate database. In response to the increasing demands of modern education, efficient and accessible retrieval of materials for educators and students is essential. This paper presents a hybrid model based on the transformer framework and utilizing an API for an existing large language model (LLM)/chatbot. This integration ensures precise responses drawn from a comprehensive educational materials database. The model architecture uses mathematically defined algorithms for precise functions that enable deep text processing through advanced word embedding methods. This approach improves accuracy in natural language processing and ensures both high efficiency and adaptability. Therefore, this paper not only provides a technical solution to a prevalent problem but also highlights the potential for the continued development and integration of emerging technologies in education. The aim is to create a more efficient, transparent, and accessible educational environment. The importance of this research lies in its ability to streamline material access, benefiting the global scientific community and contributing to the continuous advancement of educational technology.
Causes of Summer Ozone Pollution Events in Jinan, East China: Local Photochemical Formation or Regional Transport?
Authors: Wang Baolin , Sun Yuchun , Sun Lei , Liu Zhenguo , Wang Chen , Zhang Rui , Zhu Chuanyong , Yang Na , Fan Guolan , Sun Xiaoyan , Xia Zhiyong , Xu Hongyu , Pan Guang , Zhang Zhanchao , Yan Guihuan , Xu Chongqing
Published: February 2024

Simultaneous measurements of atmospheric volatile organic compounds (VOCs), conventional gases and meteorological parameters were performed at an urban site in Jinan, East China, in June 2021 to explore the formation and evolution mechanisms of summertime ozone (O3) pollution events. O3 Episode Ⅰ, O3 Episode II, and non-O3 episodes were identified based on the China Ambient Air Quality Standards and the differences in precursor concentrations. The O3 concentrations in Episode I and Episode II were 145.4 μg/m3 and 166.4 μg/m3, respectively, which were significantly higher than that in non-O3 episode (90 μg/m3). For O3 precursors, VOCs and NOx concentrations increased by 48% and 34% in Episode I, and decreased by 21% and 27% in Episode II compared to non-O3 episode days. The analysis of the m,p-xylene to ethylbenzene ratio (X/E) and OH exposure demonstrated that the aging of the air masses in Episode II was significantly higher than the other two episodes, and the differences could not be explained by localized photochemical consumption. Therefore, we speculate that the high O3 concentrations in Episode II were driven by the regional transport of O3 and its precursors. Backward trajectory simulations indicated that the air masses during Episode II were concentrated from the south. In contrast, the combination of high precursor concentrations and favorable meteorological conditions (high temperatures and low humidity) led to an excess of O3 in Episode I. Positive matrix factorization (PMF) model results indicated that increased emissions from combustion and gasoline vehicle exhausts contributed to the elevated concentrations of VOCs in Episode I, and solvent usage may be an important contributor to O3 formation. The results of this study emphasize the importance of strengthening regional joint control of O3 and its precursors with neighboring cities, especially in the south, which is crucial for Jinan to mitigate O3 pollution.
Charge Scheduling of Electric Vehicle Fleets: Maximizing Battery Remaining Useful Life Using Machine Learning Models
Authors: Geerts David , Medina Róbinson , van Sark Wilfried , Wilkins Steven
Published: February 2024

Reducing greenhouse emissions can be done via the electrification of the transport industry. However, there are challenges related to the electrification such as the lifetime of vehicle batteries as well as limitations on the charging possibilities. To cope with some of these challenges, a charge scheduling method for fleets of electric vehicles is presented. Such a method assigns the charging moments (i.e., schedules) of fleets that have more vehicles than chargers. While doing the assignation, the method also maximizes the total Remaining Useful Life (RUL) of all the vehicle batteries. The method consists of two optimization algorithms. The first optimization algorithm determines charging profiles (i.e., charging current vs time) for individual vehicles. The second algorithm finds the charging schedule (i.e., the order in which vehicles are connected to a charger) that maximizes the RUL in the batteries of the entire fleet. To reduce the computational effort of predicting the battery RUL, the method uses a Machine Learning (ML) model. Such a model predicts the RUL of an individual battery while taking into account common stress factors and fabrication-related differences per battery. Simulation results show that charging a single vehicle as late as possible maximizes the RUL of that single vehicle, due to the lower battery degradation. Simulations also show that the ML model accurately predicts the RUL, while taking into account fabrication-related variability in the battery. Additionally, it was shown that this method schedules the charging moments of a fleet, leading to an increased total RUL of all the batteries in the vehicle fleet.
Application of Adipose Stem Cells in 3D Nerve Guidance Conduit Prevents Muscle Atrophy and Improves Distal Muscle Compliance in a Peripheral Nerve Regeneration Model
Authors: Trâmbițaș Cristian , Cordoș Bogdan Andrei , Dorobanțu Dorin Constantin , Vintilă Cristian , Ion Alexandru Petru , Pap Timea , Camelia David , Puiac Claudiu , Arbănași Emil Marian , Ciucanu Claudiu Constantin , Mureșan Adrian Vasile , Arbănași Eliza Mihaela , Russu Eliza
Published: February 2024

Background: Peripheral nerve injuries (PNIs) represent a significant clinical problem, and standard approaches to nerve repair have limitations. Recent breakthroughs in 3D printing and stem cell technologies offer a promising solution for nerve regeneration. The main purpose of this study was to examine the biomechanical characteristics in muscle tissue distal to a nerve defect in a murine model of peripheral nerve regeneration from physiological stress to failure. Methods: In this experimental study, we enrolled 18 Wistar rats in which we created a 10 mm sciatic nerve defect. Furthermore, we divided them into three groups as follows: in Group 1, we used 3D nerve guidance conduits (NGCs) and adipose stem cells (ASCs) in seven rats; in Group 2, we used only 3D NGCs for seven rats; and in Group 3, we created only the defect in four rats. We monitored the degree of atrophy at 4, 8, and 12 weeks by measuring the diameter of the tibialis anterior (TA) muscle. At the end of 12 weeks, we took the TA muscle and analyzed it uniaxially at 10% stretch until failure. Results: In the group of animals with 3D NGCs and ASCs, we recorded the lowest degree of atrophy at 4 weeks, 8 weeks, and 12 weeks after nerve reconstruction. At 10% stretch, the control group had the highest Cauchy stress values compared to the 3D NGC group (0.164 MPa vs. 0.141 MPa, p = 0.007) and the 3D NGC + ASC group (0.164 MPa vs. 0.123 MPa, p = 0.007). In addition, we found that the control group (1.763 MPa) had the highest TA muscle stiffness, followed by the 3D NGC group (1.412 MPa), with the best muscle elasticity showing in the group in which we used 3D NGC + ASC (1.147 MPa). At failure, TA muscle samples from the 3D NGC + ASC group demonstrated better compliance and a higher degree of elasticity compared to the other two groups (p = 0.002 and p = 0.008). Conclusions: Our study demonstrates that the combination of 3D NGC and ASC increases the process of nerve regeneration and significantly improves the compliance and mechanical characteristics of muscle tissue distal to the injury site in a PNI murine model.
Establishment of a Human Immunocompetent 3D Tissue Model to Enable the Long-Term Examination of Biofilm–Tissue Interactions
Authors: Murkar Rasika , von Heckel Charlotte , Walles Heike , Moch Theresia Barbara , Arens Christoph , Davaris Nikolaos , Weber André , Zuschratter Werner , Baumann Sönke , Reinhardt Jörg , Kopp Sascha
Published: February 2024

Different studies suggest an impact of biofilms on carcinogenic lesion formation in varying human tissues. However, the mechanisms of cancer formation are difficult to examine in vivo as well as in vitro. Cell culture approaches, in most cases, are unable to keep a bacterial steady state without any overgrowth. In our approach, we aimed to develop an immunocompetent 3D tissue model which can mitigate bacterial outgrowth. We established a three-dimensional (3D) co-culture of human primary fibroblasts with pre-differentiated THP-1-derived macrophages on an SIS-muc scaffold which was derived by decellularisation of a porcine intestine. After establishment, we exposed the tissue models to define the biofilms of the Pseudomonas spec. and Staphylococcus spec. cultivated on implant mesh material. After 3 days of incubation, the cell culture medium in models with M0 and M2 pre-differentiated macrophages presented a noticeable turbidity, while models with M1 macrophages presented no noticeable bacterial growth. These results were validated by optical density measurements and a streak test. Immunohistology and immunofluorescent staining of the tissue presented a positive impact of the M1 macrophages on the structural integrity of the tissue model. Furthermore, multiplex ELISA highlighted the increased release of inflammatory cytokines for all the three model types, suggesting the immunocompetence of the developed model. Overall, in this proof-of-principle study, we were able to mitigate bacterial overgrowth and prepared a first step for the development of more complex 3D tissue models to understand the impact of biofilms on carcinogenic lesion formation.
Running-Time Analysis of Brain Storm Optimization Based on Average Gain Model
Authors: Mai Guizhen , Liu Fangqing , Hong Yinghan , Liu Dingrong , Su Junpeng , Yang Xiaowei , Huang Han
Published: February 2024

The brain storm optimization (BSO) algorithm has received increased attention in the field of evolutionary computation. While BSO has been applied in numerous industrial scenarios due to its effectiveness and accessibility, there are few theoretical analysis results about its running time. Running-time analysis can be conducted through the estimation of the upper bounds of the expected first hitting time to evaluate the efficiency of BSO. This study estimates the upper bounds of the expected first hitting time on six single individual BSO variants (BSOs with one individual) based on the average gain model. The theoretical analysis indicates the following results. (1) The time complexity of the six BSO variants is O(n) in equal coefficient linear functions regardless of the presence or absence of the disrupting operator, where n is the number of the dimensions. Moreover, the coefficient of the upper bounds on the expected first hitting time shows that the single individual BSOs with the disrupting operator require fewer iterations to obtain the target solution than the single individual BSOs without the disrupting operator. (2) The upper bounds on the expected first hitting time of single individual BSOs with the standard normally distributed mutation operator are lower than those of BSOs with the uniformly distributed mutation operator. (3) The upper bounds on the expected first hitting time of single individual BSOs with the U−12,12 mutation operator are approximately twice those of BSOs with the U(−1,1) mutation operator. The corresponding numerical results are also consistent with the theoretical analysis results.
Sustainable and Efficient Structural Systems for Tall Buildings: Exploring Timber and Steel–Timber Hybrids through a Case Study
Authors: Ascione Fabrizio , Esposito Francesco , Iovane Giacomo , Faiella Diana , Faggiano Beatrice , Mele Elena
Published: February 2024

The paper focuses on tall timber buildings. The major aim of this paper is to identify the most sustainable and efficient structural system to increase the height of timber buildings, also considering steel–timber hybrid structures. First of all, a brief review of tall buildings’ evolution is presented to understand why tall timber buildings are considered nowadays and which are the most adopted structural solutions. Then, the case study of the tallest timber building in the world is selected and utilized as an archetype. Once the model has been validated, seven alternative structural systems are considered by varying the horizontal load resisting system and preserving the same member cross-sections as the reference building. The variants are tested and compared in terms of material consumption, vibration characteristics and lateral load response. Using the best structural system, the height of the building is increased, pushing the structures beyond the current limits and identifying the most efficient option. The idea is to preserve the column cross-sections and balance the increase in gravity loads due to the additional floors by replacing the concrete floors with timber counterparts. With the same structural system, equivalent steel–timber hybrid solutions are finally tested and compared in terms of sustainability to timber-only counterparts and to the original project. The results of analyses show that the use of steel elements combined with timber can lead to optimized and sustainable structural solutions.
Interface Modulation of CoNi Alloy Decorated with Few-Layer Reduced Graphene Oxide for High-Efficiency Microwave Absorption
Authors: Xie Hai , Li Jinmei , Zhang Yaoming , Yang Juan , Wang Tingmei , Wang Qihua
Published: February 2024

Metal-organic frameworks (MOFs)-derived microwave absorbers with tunable components and microstructures show great potential in microwave absorption. Herein, we report a facile thermal reduction approach for synthesizing CoNi alloy/reduced graphene oxide (CoNi/rGO) composites from bimetallic CoNi-MOFs. By tuning the ratio of graphene oxide (GO) in the precursors, the resulting CoNi/rGO-2 composite demonstrates optimal microwave absorption performance with a minimum reflection loss (RLmin) of −66.2 dB at 7.6 GHz in the C band. Moreover, the CoNi/rGO-2 with 50 wt% filler loading achieves a maximum effective absorption bandwidth (EAB) of 6.8 GHz (10.6–17.4 GHz) at a thickness of 2.5 mm, almost spanning the entire Ku band and a portion of the X band. The outstanding performance of CoNi/rGO-2 is ascribed to the high magnetic loss from the CoNi alloy and the incorporation of rGO, which induces interfacial polarization to enhance the dielectric loss and improve the impedance matching of composite. These favorable findings highlight the considerable potential and superiority of the CoNi/rGO-2 composite as an electromagnetic wave absorption material. This work sets forth a viable strategy for designing high-efficiency alloy/rGO absorbers.
Preoperative Assessment of Medication-Related Osteonecrosis of the Jaw Using [18F]fluoride Positron Emission Tomography (PET)/CT and [18F]fluorodeoxyglucose PET/MRI in Correlation with Histomorphometry and Micro-CT—A Prospective Comparative Study
Authors: Reinert Christian Philipp , Pfannenberg Christina , Gückel Brigitte , Dittmann Helmut , la Fougère Christian , Nikolaou Konstantin , Reinert Siegmar , Schönhof Rouven , Hoefert Sebastian
Published: February 2024

Objectives: The purpose of this study was to investigate the imaging characteristics of medication-related osteonecrosis of the jaw (MRONJ) using [18F]fluoride positron emission tomography/computed tomography (PET/CT) and [18F]fluorodeoxyglucose (FDG) PET/magnetic resonance imaging (MRI) for preoperative assessment and to correlate them with microarchitectural and histomorphometric data with respect to clinical findings. Methods: Twelve patients (five female; mean age 75 ± 7.6 yr) with symptomatic MRONJ underwent both scans on the same day, and imaging findings were used to plan surgical interventions for seven patients. Bone tracer uptake was classified as high, medium, or low, and surgical samples were evaluated using Micro-CT and histomorphometric analysis. Results: CT showed medullary sclerosis in all patients, and MRI revealed gadolinium enhancement in four patients. PET imaging revealed remarkably elevated [18F]fluoride uptake and moderately increased [18F]FDG uptake in MRONJ compared to healthy jawbones, with both differences being statistically significant. [18F]fluoride uptake was associated with necrosis, bacteria, and inflammatory tissue. Micro-CT data did not show significant differences, but histomorphometric analysis revealed higher osteocyte and lacunae densities in the high [18F]fluoride uptake group, and more necrotic bone in the medium [18F]fluoride uptake group. Bacteria were observed in all areas. Conclusions: In summary, [18F]fluoride PET accurately identified MRONJ extent, revealing functional changes in jawbone remodeling not visible on CT. [18F]FDG PET showed differences in bone and soft tissue, though less pronounced. This method aids in evaluating disease activity and guiding treatment planning, requiring further research for optimal surgical approaches based on tracer uptake.
AI-Assisted Programming Tasks Using Code Embeddings and Transformers
Authors: Kotsiantis Sotiris , Verykios Vassilios , Tzagarakis Manolis
Published: February 2024

This review article provides an in-depth analysis of the growing field of AI-assisted programming tasks, specifically focusing on the use of code embeddings and transformers. With the increasing complexity and scale of software development, traditional programming methods are becoming more time-consuming and error-prone. As a result, researchers have turned to the application of artificial intelligence to assist with various programming tasks, including code completion, bug detection, and code summarization. The utilization of artificial intelligence for programming tasks has garnered significant attention in recent times, with numerous approaches adopting code embeddings or transformer technologies as their foundation. While these technologies are popular in this field today, a rigorous discussion, analysis, and comparison of their abilities to cover AI-assisted programming tasks is still lacking. This article discusses the role of code embeddings and transformers in enhancing the performance of AI-assisted programming tasks, highlighting their capabilities, limitations, and future potential in an attempt to outline a future roadmap for these specific technologies.
Enhancing Bromelain Recovery from Pineapple By-Products: A Sustainable Approach for Value Addition and Waste Reduction
Authors: Chiarelli Peter G. , Martinez Bismarck , Nakamura Takashi , Mis Solval Kevin
Published: February 2024

Pineapple by-products are good sources of bromelain, a complex enzyme with commercial applications. This study evaluated the feasibility of producing bromelain powders from pineapple waste using an organic solvent-free approach. Pineapple by-products (from var. MD2), including cores, peels, crowns, stems, and basal stems, were homogenized with deionized water, and the pH of the mixture was adjusted to 4.5 and 9 (isoelectric points of fruit bromelain and stem bromelain), clarified, ultra-filtered, and freeze-dried to produce bromelain powders. The enzymatic activity of the bromelain powders was measured using the gelatin and casein digestion methods. The bromelain powders from the crowns did not show significant enzymatic activity (p < 0.05). Meanwhile, bromelain powders produced from the cores and peels had an enzymatic activity of 694 gelatin digesting units (GDU)/g and 124 casein digesting units (CDU)/mg, and 1179 GDU/g and 217 CDU/mg, respectively. Bromelain powders from the basal stems showed the highest enzymatic activity (2909 GDU/g and 717 CDU/mg). Increasing the pH of the liquid mixture before the purification and freeze drying significantly (p < 0.05) reduced the enzymatic activity of the bromelain powders. Using a practical and organic solvent-free approach, this study demonstrates the feasibility of producing bromelain powders with high enzymatic activity from pineapple waste.
Rainfall Infiltration through Stratified Colluvial Deposits: Analytical Approach vs. Numerical Modelling
Authors: Paronuzzi Paolo , Fedrigo Dario , Bolla Alberto
Published: February 2024

This work investigates the rainfall infiltration process within homogeneous and stratified colluvial deposits caused by short (1–3 h) and intense (40–90 mm/h) rainfall, using both analytical and numerical infiltration modelling. The findings of the investigation demonstrate that the classic Green–Ampt model can be employed effectively to study homogeneous colluvial covers with permeability equal to or lower than kw = 10−5 m/s and that are subject to a 1 h rainfall with intensity I ≥ 45–50 mm/h. In these circumstances, a top-down saturation front forms within the colluvial deposit, leading to the saturation of a 70–100 cm-thick layer. This critical condition occurs every 5–10 years in the mountain area of the Friuli Venezia Giulia Region (NE Italy), which corresponds to a lower return period of critical hydrologic events when compared with other mountain basins in the Alps due to the higher initial degree of saturation characterising colluvial covers in this area (70–95%). When analysing stratified colluvial covers, the Dagan–Bresler approximate model, as well as the numerical modelling, emphasised the strong influence that abrupt variations in the permeability of the various soil layers have on the infiltration process at depth. In particular, the presence of a top organic soil horizon that is rich in macro-pores and is characterised by a higher permeability (k = 10−4 m/s) actually reduces the possibility of surficial ponding, which is the basic condition of the “piston” models. The highly permeable top soil allows for a rapid downward infiltration up to contact with the underlying colluvial material, which is less permeable (k = 10−5 m/s). Therefore, a perched water table forms starting from the organic soil–colluvium interface, originating pore–water overpressures within the colluvial deposit, with maximum values in the order of 5–10 kPa.
Hydrogeological Characteristics of the Makaresh Carbonate Karst Massif (Central Albania)
Authors: Eftimi Romeo , Liso Isabella Serena , Parise Mario
Published: February 2024

Carbonate rocks cover about 23% of Albania, with exploitable karst water resources estimated at 2.84 × 109 m3/year (about 65% of the total exploitable groundwater resources in the country). The Kruja tectonic zone is characterized by the presence of SE–NW-oriented carbonate structures, rich in fresh and thermal groundwaters. More than 80% of the thermal springs in Albania are present in this tectonic zone. One of its most interesting carbonate structures, with the presence of both cold and thermal waters, is the small karst structure of Makaresh, with a surface of 22 km2. The purpose of this article is to describe the hydrogeological characteristics of this massif; based on the physico-chemical characteristics, groundwaters of the study area are classified as cold waters (belonging to the local flow system) and thermal waters (originating in intermediate/deep flow systems). The former are mainly of HCO3-Ca or HCO3-Ca-Mg type (electrical conductivity 580–650 μS/cm, Temperature 13.9–16.6 °C). Thermal waters are mainly of the Cl-Na-Ca type (EC 7200–7800 μS/cm, T 18.5–22.5 °C); they are further characterized by high hydrogen sulfide concentration, up to about 350 mg/L. The presence of two groundwater types in the Makaresh massif is connected to the presence of two groundwater circulation systems. The main factors of the groundwater physico-chemical quality are the dissolution of rocks and minerals contained therein, the presence of hypogenic speleogenesis, and the mixing of the groundwater of the two systems. The hydrogeological studies proved that karst rocks contain considerable freshwater resources, partly used for water supply. Thermal waters are not currently exploited due to their temperature, but they are potentially suitable for thermal uses by drilling boreholes to a depth of about 1000 m.
Genome-Wide Analysis of the Oat (Avena sativa) HSP90 Gene Family Reveals Its Identification, Evolution, and Response to Abiotic Stress
Authors: Peng Jinghan , Liu Siyu , Wu Jiqiang , Liu Tianqi , Liu Boyang , Xiong Yi , Zhao Junming , You Minghong , Lei Xiong , Ma Xiao
Published: February 2024

Oats (Avena sativa) are an important cereal crop and cool-season forage worldwide. Heat shock protein 90 (HSP90) is a protein ubiquitously expressed in response to heat stress in almost all plants. To date, the HSP90 gene family has not been comprehensively reported in oats. Herein, we have identified twenty HSP90 genes in oats and elucidated their evolutionary pathways and responses to five abiotic stresses. The gene structure and motif analyses demonstrated consistency across the phylogenetic tree branches, and the groups exhibited relative structural conservation. Additionally, we identified ten pairs of segmentally duplicated genes in oats. Interspecies synteny analysis and orthologous gene identification indicated that oats share a significant number of orthologous genes with their ancestral species; this implies that the expansion of the oat HSP90 gene family may have occurred through oat polyploidization and large fragment duplication. The analysis of cis-acting elements revealed their influential role in the expression pattern of HSP90 genes under abiotic stresses. Analysis of oat gene expression under high-temperature, salt, cadmium (Cd), polyethylene glycol (PEG), and abscisic acid (ABA) stresses demonstrated that most AsHSP90 genes were significantly up-regulated by heat stress, particularly AsHSP90-7, AsHSP90-8, and AsHSP90-9. This study offers new insights into the amplification and evolutionary processes of the AsHSP90 protein, as well as its potential role in response to abiotic stresses. Furthermore, it lays the groundwork for understanding oat adaptation to abiotic stress, contributing to research and applications in plant breeding.
A Simple, Fast, Sensitive LC-MS/MS Method to Quantify NAD(H) in Biological Samples: Plasma NAD(H) Measurement to Monitor Brain Pathophysiology
Authors: Ishima Tamaki , Kimura Natsuka , Kobayashi Mizuki , Nagai Ryozo , Osaka Hitoshi , Aizawa Kenichi
Published: February 2024

Nicotinamide adenine dinucleotide (NAD) is a cofactor in redox reactions and an essential mediator of energy metabolism. The redox balance between NAD+ and NADH affects various diseases, cell differentiation, and aging, and in recent years there has been a growing need for measurement techniques with improved accuracy. However, NAD(H) measurements, representing both NAD+ and NADH, have been limited by the compound’s properties. We achieved highly sensitive simultaneous measurement of NAD+ and NADH under non-ion pairing, mobile phase conditions of water, or methanol containing 5 mM ammonium acetate. These were achieved using a simple pre-treatment and 7-min analysis time. Use of the stable isotope 13C5-NAD+ as an internal standard enabled validation close to BMV criteria and demonstrated the robustness of NAD(H) determination. Measurements using this method showed that brain NAD(H) levels correlate strongly with plasma NAD(H) levels in the same mouse, indicating that NAD(H) concentrations in brain tissue are reflected in plasma. As NAD(H) is involved in various neurodegenerative diseases and cerebral ischemia, as well as brain diseases such as mitochondrial myopathies, monitoring changes in NADH levels in plasma after drug administration will be useful for development of future diagnostics and therapeutics.
The Model of Interstitial Cystitis for Evaluating New Molecular Strategies of Interstitial Regeneration in Humans
Authors: Mormone Elisabetta , Cisternino Antonio , Capone Lorenzo , Caradonna Eugenio , Sbarbati Andrea
Published: February 2024

Given the recent evidence in the clinical application of regenerative medicine, mostly on integumentary systems, we focused our interests on recent bladder regeneration approaches based on mesenchymal stem cells (MSCs), platelet-rich plasma (PRP), and hyaluronic acid (HA) in the treatment of interstitial cystitis/bladder pain syndrome (IC/BPS) in humans. IC/BPS is a heterogeneous chronic disease with not-well-understood etiology, characterized by suprapubic pain related to bladder filling and urothelium dysfunction, in which the impairment of immunological processes seems to play an important role. The histopathological features of IC include ulceration of the mucosa, edema, denuded urothelium, and increased detection of mast cells and other inflammatory cells. A deeper understanding of the molecular mechanism underlying this disease is essential for the selection of the right therapeutic approach. In fact, although various therapeutic strategies exist, no efficient therapy for IC/BPS has been discovered yet. This review gives an overview of the clinical and pathological features of IC/BPS, with a particular focus on the molecular pathways involved and a special interest in the ongoing few investigational therapies in IC/BPS, which use new regenerative medicine approaches, and their synergetic combination. Good knowledge of the molecular aspects related to stem cell-, PRP-, and biomaterial-based treatments, as well as the understanding of the molecular mechanism of this pathology, will allow for the selection of the right and best use of regenerative approaches of structures involving connective tissue and epithelia, as well as in other diseases.