bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of the value of adjuvant radiotherapy for treatment of gastric adenocarcinoma based on pattern of post-surgical progression
Authors: Wang Peng , Zhou Haihua , Han Gaohua , Ni Qingtao , Dai Shengbin , Huang Junxing , Dai Chunlei , Yu Lei
Published: July 2021

Purpose

To assess the value of adjuvant radiotherapy for treatment of gastric adenocarcinoma and to investigate subgroups of patients suitable for adjuvant radiotherapy.

Methods and materials

Data from 785 patients with gastric adenocarcinoma who had undergone D1/D2 radical resection and adjuvant chemotherapy were collected, the site of first progression was determined, and the relationship between the rate of local recurrence and clinicopathologic features was analyzed.

Results

By the end of the follow-up period, progression was observed in 405 patients. Local recurrence was observed as the first progression in 161 cases. The local recurrence rate was significantly lower than the non-local progression rate (20.5% vs 31.5%, p=0.007). Multivariate Cox regression analysis showed a significant relationship among degree of differentiation, T stage, N stage, and rate of local recurrence.

Conclusions

Not all patients with gastric carcinoma required adjuvant radiotherapy. However, patients with poorly differentiated cancer cells, advanced T stage (T3/T4), and positive lymph nodes, which included patients in the T4N1-2M0 subgroup, were recommended for adjuvant radiotherapy.
Inherited unbalanced translocation (4p16.3p15.32 duplication/8p23.3p23.2deletion) in the four generation pedigree with intellectual disability/developmental delay
Authors: Hao Dongmei , Li Yajuan , Chen Lisha , Wang Xiliang , Wang Mengxing , Yu Yuexin
Published: July 2021

Chromosomal copy number variants (CNVs) are an important cause of congenital malformations and mental retardation. This study reported a large Chinese pedigree (4-generation, 76 members) with mental retardation caused by chromosome microduplication/microdeletion. There were 10 affected individuals with intellectual disability (ID), developmental delay (DD), and language delay phenotypes. SNP array analysis was performed in the proband and eight patients and found all of them had a microduplication of chromosome 4p16.3p15.2 and a microdeletion of chromosome 8p23.3p23.2. The high-resolution karyotyping analysis of the proband had unbalanced karyotype [46, XY, der(8)t(4;8)(p15.2;p23.1)mat], his mother had balanced karyotype [46, XX, t(4;8) (p15.2;p23.1)], whereas his father had normal karyotype [46,XY]. Fluorescence in situ hybridization (FISH) analysis further confirmed that the proband’s mother had a balanced translocation between the short arm terminal segment of chromosome 4 and the short arm end segment of chromosome 8, ish t(4;8)(8p + ,4q + ;4p + ,8q +). In conclusion, all the patients inherited chromosomes 8 with 4p16.3p15.2 duplication and 8p23.3p23.2 deletion from their parental balanced translocation, which might be the cause of the prevalence of intellectual disability. Meanwhile, 8p23.3p23.2 deletion, rather than 4p16.3p15.2 duplication might cause a more severe clinical syndrome.
Development of a rabies virus-based retrograde tracer with high trans-monosynaptic efficiency by reshuffling glycoprotein
Authors: Jia Fan , Li Li , Liu Haizhou , Lv Pei , Shi Xiangwei , Wu Yang , Ling Chen , Xu Fuqiang
Published: July 2021

Rabies virus (RV) is the most widely used vector for mapping neural circuits. Previous studies have shown that the RV glycoprotein can be a target to improve the retrograde transsynaptic tracing efficiency. However, the current versions still label only a small portion of all presynaptic neurons. Here, we reshuffled the oG sequence, a chimeric glycoprotein, with positive codon pair bias score (CPBS) based on bioinformatic analysis of mouse codon pair bias, generating ooG, a further optimized glycoprotein. Our experimental data reveal that the ooG has a higher expression level than the oG in vivo, which significantly increases the tracing efficiency by up to 12.6 and 62.1-fold compared to oG and B19G, respectively. The new tool can be used for labeling neural circuits Therefore, the approach reported here provides a convenient, efficient and universal strategy to improve protein expression for various application scenarios such as trans-synaptic tracing efficiency, cell engineering, and vaccine and oncolytic virus designs.
MiR-585-3p suppresses tumor proliferation and migration by directly targeting CAPN9 in high grade serous ovarian cancer
Authors: Lu Xiaoyuan , Li Guilin , Liu Sicong , Wang Haihong , Chen Buze
Published: July 2021

Background

Aberrant expression of microRNAs (miRNAs) contributes to the development of high grade serous ovarian cancer (HGSOC). However, the molecular mechanism by which miRNA-585-3p mediates high-grade serous ovarian carcinogenesis is unclear. This study aims to investigate the specific mechanism of action of miR-585-3p in HGSOC.

Methods

Expression of miR-585-3p in HGSOC tissues and cell lines was detected by qRT-PCR. Cell viability and migration were detected using MTT and transwell system. The expression of target genes and target proteins of miR-585-3p was detected by dual luciferase reporter assay and western blot.

Results

The expression of miR-585-3p was significantly lower in HGSOC tissues and cells than in normal ovarian tissues and cell lines. In HGSOC tissues, CAPN9 expression was inversely correlated with miR-585-3p expression. MiR-585-3p inhibited the proliferation and migration of HGSOC cells. MiR-585-3p bound to the 3'-untranslated region (UTR) of CAPN9 and inhibits CAPN9 expression. Overexpression of CAPN9 reduced the inhibitory effect of miR-585-3p in HGSOC cells.

Conclusions

miR-585-3p is significantly down-regulated in HGSOC tissues and cell lines. MiR-585-3p inhibits the proliferation and migration of HGSOC cells by targeting CAPN9.
Diagnostic yield of routine daily blood culture in patients on veno-arterial extracorporeal membrane oxygenation
Authors: de Roux Quentin , Renaudier Marie , Bougouin Wulfran , Boccara Johanna , Fihman Vincent , Lepeule Raphaël , Cherait Chamsedine , Fiore Antonio , Hemery François , Decousser Jean-Winoc , Langeron Olivier , Mongardon Nicolas
Published: July 2021

Background

Bloodstream infections (BSIs) are frequent on veno-arterial extracorporeal membrane oxygenation (V-A ECMO). Performing routine blood cultures (BCs) may identify early paucisymptomatic BSIs. We investigated the contribution of systematic daily BCs to detect BSIs on V-A ECMO.

Methods

This was a retrospective study including all adult patients requiring V-A ECMO and surviving more than 24 h. Our protocol included routine daily BCs, from V-A ECMO insertion up to 5 days after withdrawal; other BCs were performed on-demand.

Results

On the 150 V-A ECMO included, 2146 BCs were performed (1162 routine and 984 on-demand BCs); 190 (9%) were positive, including 68 contaminants. Fifty-one (4%) routine BCs revealed BSIs; meanwhile, 71 (7%) on-demand BCs revealed BSIs (p = 0.005). Performing routine BCs was negatively associated with BSIs diagnosis (OR 0.55, 95% CI [0.38; 0.81],p = 0.002). However, 16 (31%) BSIs diagnosed by routine BCs would have been missed by on-demand BCs. Independent variables for BSIs diagnosis after routine BCs were: V-A ECMO for cardiac graft failure (OR 2.43, 95% CI [1.20; 4.92],p = 0.013) and sampling with on-going antimicrobial therapy (OR 2.15, 95% CI [1.08; 4.27],p = 0.029) or renal replacement therapy (OR 2.05, 95% CI [1.10; 3.81],p = 0.008). Without these three conditions, only two BSIs diagnosed with routine BCs would have been missed by on-demand BCs sampling.

Conclusions

Although routine daily BCs are less effective than on-demand BCs and expose to contamination and inappropriate antimicrobial therapy, a policy restricted to on-demand BCs would omit a significant proportion of BSIs. This argues for a tailored approach to routine daily BCs on V-A ECMO, based on risk factors for positivity.
Estimating the wave 1 and wave 2 infection fatality rates from SARS-CoV-2 in India
Authors: Purkayastha Soumik , Kundu Ritoban , Bhaduri Ritwik , Barker Daniel , Kleinsasser Michael , Ray Debashree , Mukherjee Bhramar
Published: July 2021

Objective

There has been much discussion and debate around the underreporting of COVID-19 infections and deaths in India. In this short report we first estimate the underreporting factor for infections from publicly available data released by the Indian Council of Medical Research on reported number of cases and national seroprevalence surveys. We then use a compartmental epidemiologic model to estimate the undetected number of infections and deaths, yielding estimates of the corresponding underreporting factors. We compare the serosurvey based ad hoc estimate of the infection fatality rate (IFR) with the model-based estimate. Since the first and second waves in India are intrinsically different in nature, we carry out this exercise in two periods: the first wave (April 1, 2020–January 31, 2021) and part of the second wave (February 1, 2021–May 15, 2021). The latest national seroprevalence estimate is from January 2021, and thus only relevant to our wave 1 calculations.

Results

Both wave 1 and wave 2 estimates qualitatively show that there is a large degree of “covert infections” in India, with model-based estimated underreporting factor for infections as 11.11 (95% credible interval (CrI) 10.71–11.47) and for deaths as 3.56 (95% CrI 3.48–3.64) for wave 1. For wave 2, underreporting factor for infections escalate to 26.77 (95% CrI 24.26–28.81) and to 5.77 (95% CrI 5.34–6.15) for deaths. If we rely on only reported deaths, the IFR estimate is 0.13% for wave 1 and 0.03% for part of wave 2. Taking underreporting of deaths into account, the IFR estimate is 0.46% for wave 1 and 0.18% for wave 2 (till May 15). Combining waves 1 and 2, as of May 15, while India reported a total of nearly 25 million cases and 270 thousand deaths, the estimated number of infections and deaths stand at 491 million (36% of the population) and 1.21 million respectively, yielding an estimated (combined) infection fatality rate of 0.25%. There is considerable variation in these estimates across Indian states. Up to date seroprevalence studies and mortality data are needed to validate these model-based estimates.
Pharmacokinetic evaluation of Chalcone derivatives with antimalarial activity in New Zealand White Rabbits
Authors: Sinha Shweta , Prakash Ajay , Medhi Bikash , Sehgal Alka , Batovska Daniela I. , Sehgal Rakesh
Published: July 2021

Objective

Malaria is a major global health concern with the urgent need for new treatment alternatives due to the alarming increase of drug-resistant Plasmodium strains. Chalcones and its derivatives are important pharmacophores showing antimalarial activity. Determination of the pharmacokinetic variables at the preliminary step of drug development for any drug candidates is an essential component of in vivo antimalarial efficacy tests. Substandard pharmacokinetic variables are often responsible for insufficient therapeutic effect. Therefore, three chalcone derivatives, 1, 2, and 3, having antimalarial potency were studied further for potential therapeutic efficacy.

Results

In vivo pharmacokinetic studies of these three derivatives were performed on New Zealand White rabbits. The three derivatives were administered intra-peritoneally or orally at effective dose concentration and blood samples at different time points were collected. The determination of drug concentration was done through reverse phase-high performance liquid chromatography. The peak plasma concentration of derivative 1, 2, and 3 were 1.96 ± 0.46 µg/mL (intraperitoneal route), 69.89 ± 5.49 µg/mL (oral route), and 3.74 ± 1.64 µg/mL (oral route). The results indicate a very low bioavailability of these derivatives. The present study gives a benchmark to advance the investigation of more derivatives in order to revamp the pharmacokinetic variables while maintaining both potency and metabolic constancy.
Lewy Body Dementia Association’s Industry Advisory Council: proceedings of the second annual meeting
Authors: Goldman Jennifer G. , Boeve Bradley F. , Armstrong Melissa J. , Galasko Doug R. , Galvin James E. , Irwin David J. , Leverenz James B. , Marder Karen , Abler Victor , Biglan Kevin , Irizarry Michael C. , Keller Bill , Lai Robert , Munsie Leanne , Belleville Michael , Chaney Ondrea , Richard Ian , Taylor Angela , Graham Todd
Published: July 2021

In 2019, the Lewy Body Dementia Association formed an Industry Advisory Council to bring together a collaborative group of stakeholders with the goal of accelerating clinical research into Lewy body dementia treatments. At the second annual meeting of the Industry Advisory Council, held virtually on June 18, 2020, the key members presented ongoing and planned efforts toward the council’s goals. The meeting also featured a discussion about the effects of the COVID-19 pandemic on Lewy body dementia clinical research, lessons learned from that experience, and how those lessons can be applied to the design and conduct of future clinical trials. This report provides a brief summary of the meeting proceedings with a focus on efforts to improve and adapt future Lewy body dementia clinical research.
Pre-existing asthma as a comorbidity does not modify cytokine responses and severity of COVID-19
Authors: Luo Jian , Chen Yi-Ling , Chen Wentao , Duncan David A. , Mentzer Alexander , Knight Julian C. , Ogg Graham , Klenerman Paul , Pavord Ian D. , Xue Luzheng
Published: July 2021

Background

A significant portion of COVID-19 sufferers have asthma. The impacts of asthma on COVID-19 progression are still unclear but a modifying effect is plausible as respiratory viruses are acknowledged to be an important trigger for asthma exacerbations and a different, potentially type-2 biased, immune response might occur. In this study, we compared the blood circulating cytokine response to COVID-19 infection in patients with and without asthma.

Methods

Plasma samples and clinical information were collected from 80 patients with mild (25), severe (36) or critical (19) COVID-19 and 29 healthy subjects at the John Radcliffe Hospital, Oxford, UK. The concentrations of 51 circulating proteins in the plasma samples were measured with Luminex and compared between groups.

Results

Total 16 pre-existing asthma patients were found (3 in mild, 10 in severe, and 3 in critical COVID-19). The prevalence of asthma in COVID-19 severity groups did not suggest a clear correlation between asthma and COVID-19 severity. Within the same COVID-19 severity group, no differences were observed between patients with or without asthma on oxygen saturation, CRP, neutrophil counts, and length of hospital stay. The mortality in the COVID-19 patients with asthma (12.5%) was not higher than that in patients without asthma (17.2%). No significant difference was found between asthmatic and non-asthmatic in circulating cytokine response in different COVID-19 severity groups, including the cytokines strongly implicated in COVID-19 such as CXCL10, IL-6, CCL2, and IL-8.

Conclusions

Pre-existing asthma was not associated with an enhanced cytokine response after COVID-19 infection, disease severity or mortality.
Quantitative ultrasound shear wave elastography (USWE)-measured tissue stiffness correlates with PIRADS scoring of MRI and Gleason score on whole-mount histopathology of prostate cancer: implications for ultrasound image-guided targeting approach
Authors: Ageeli Wael , Wei Cheng , Zhang Xinyu , Szewcyk-Bieda Magdalena , Wilson Jennifer , Li Chunhui , Nabi Ghulam
Published: July 2021

Objective

To correlate quantitative tissue stiffness measurements obtained by transrectal ultrasound shear wave elastography (USWE) with PI-RADS scoring of multiparametric magnetic imaging resonance (mpMRI) using Gleason scores of radical prostatectomy as a reference standard.

Patients and methods

196 men with localised prostate cancer were prospectively recruited into the study and had quantitative prostate tissue stiffness measurements in kilopascals (kPa) using transrectal USWE prior to radical prostatectomy. PI-RADS scores of mpMRI were also obtained in all the men. Imaging and histopathology of radical prostatectomy specimen were oriented to each other using patient specific customised 3D moulds to guide histopathology grossing of radical prostatectomy specimens. All included patients had confirmed PCa on TRUS-guided biopsies, had both USWE and mpMRI imaging data, and underwent radical prostatectomy. Chi-square test with 95% confidence interval was used to assess the difference between Gleason score (GS) of radical prostatectomy and PI-RADS classification, as well as GS of radical prostatectomy and stiffness (in Kpa) using USWE. The correlation coefficient (r) was calculated in order to investigate relation between PI-RADS classification and tissue stiffness in kPa.

Results

There was a statistically significant correlation between USWE-measured tissue stiffness and GS (χ2 (2, N = 196) = 23.577, p < 0.001). Also, there was a statistically significant correlation between Gleason score and PI-RADS score (χ2 (2, N = 196) = 12.838, p = 0.002). High PIRADS on MRI and high stiffness on USWE (> 100 kPa) detected more than 80% and 90% high risk prostate cancer disease. However, a weak correlation coefficient of 0.231 was observed between PI-RADS score and level of tissue stiffness measured in kPa.

Conclusion

Quantitative USWE and mpMRI using PI-RADS classification provide a good degree of prediction for Gleason score of clinically significant prostate cancer (csPCa). Stiffer lesions on ultrasound showed a weak correlation with PI-RADS scoring system. USWE could be used to target suspected prostate cancer.
mRNP granule proteins Fmrp and Dcp1a differentially regulate mRNP complexes to contribute to control of muscle stem cell quiescence and activation
Authors: Roy Nainita , Sundar Swetha , Pillai Malini , Patell-Socha Farah , Ganesh Sravya , Aloysius Ajoy , Rumman Mohammed , Gala Hardik , Hughes Simon M. , Zammit Peter S. , Dhawan Jyotsna
Published: July 2021

Background

During skeletal muscle regeneration, satellite stem cells use distinct pathways to repair damaged myofibers or to self-renew by returning to quiescence. Cellular/mitotic quiescence employs mechanisms that promote a poised or primed state, including altered RNA turnover and translational repression. Here, we investigate the role of mRNP granule proteins Fragile X Mental Retardation Protein (Fmrp) and Decapping protein 1a (Dcp1a) in muscle stem cell quiescence and differentiation.

Methods

Using isolated single muscle fibers from adult mice, we established differential enrichment of mRNP granule proteins including Fmrp and Dcp1a in muscle stem cells vs. myofibers. We investigated muscle tissue homeostasis in adult Fmr1-/- mice, analyzing myofiber cross-sectional area in vivo and satellite cell proliferation ex vivo. We explored the molecular mechanisms of Dcp1a and Fmrp function in quiescence, proliferation and differentiation in a C2C12 culture model. Here, we used polysome profiling, imaging and RNA/protein expression analysis to establish the abundance and assembly status of mRNP granule proteins in different cellular states, and the phenotype of knockdown cells.

Results

Quiescent muscle satellite cells are enriched for puncta containing the translational repressor Fmrp, but not the mRNA decay factor Dcp1a. MuSC isolated from Fmr1-/- mice exhibit defective proliferation, and mature myofibers show reduced cross-sectional area, suggesting a role for Fmrp in muscle homeostasis. Expression and organization of Fmrp and Dcp1a varies during primary MuSC activation on myofibers, with Fmrp puncta prominent in quiescence, but Dcp1a puncta appearing during activation/proliferation. This reciprocal expression of Fmrp and Dcp1a puncta is recapitulated in a C2C12 culture model of quiescence and activation: consistent with its role as a translational repressor, Fmrp is enriched in non-translating mRNP complexes abundant in quiescent myoblasts; Dcp1a puncta are lost in quiescence, suggesting stabilized and repressed transcripts. The function of each protein differs during proliferation; whereas Fmrp knockdown led to decreased proliferation and lower cyclin expression, Dcp1a knockdown led to increased cell proliferation and higher cyclin expression. However, knockdown of either Fmrp or Dcp1a led to compromised differentiation. We also observed cross-regulation of decay versus storage mRNP granules; knockdown of Fmrp enhances accumulation of Dcp1a puncta, whereas knockdown of Dcp1a leads to increased Fmrp in puncta.

Conclusions

Taken together, our results provide evidence that the balance of mRNA turnover versus utilization is specific for distinct cellular states.
Transcriptomic analysis of equine chorioallantois reveals immune networks and molecular mechanisms involved in nocardioform placentitis
Authors: El-Sheikh Ali Hossam , Loux Shavahn C. , Kennedy Laura , Scoggin Kirsten E. , Dini Pouya , Fedorka Carleigh E. , Kalbfleisch Theodore S. , Esteller-Vico Alejandro , Horohov David W. , Erol Erdal , Carter Craig N. , Smith Jackie L. , Ball Barry A.
Published: July 2021

Nocardioform placentitis (NP) continues to result in episodic outbreaks of abortion and preterm birth in mares and remains a poorly understood disease. The objective of this study was to characterize the transcriptome of the chorioallantois (CA) of mares with NP. The CA were collected from mares with confirmed NP based upon histopathology, microbiological culture and PCR for Amycolatopsis spp. Samples were collected from the margin of the NP lesion (NPL, n = 4) and grossly normal region (NPN, n = 4). Additionally, CA samples were collected from normal postpartum mares (Control; CRL, n = 4). Transcriptome analysis identified 2892 differentially expressed genes (DEGs) in NPL vs. CRL and 2450 DEGs in NPL vs. NPN. Functional genomics analysis elucidated that inflammatory signaling, toll-like receptor signaling, inflammasome activation, chemotaxis, and apoptosis pathways are involved in NP. The increased leukocytic infiltration in NPL was associated with the upregulation of matrix metalloproteinase (MMP1, MMP3, and MMP8) and apoptosis-related genes, such as caspases (CASP3 and CASP7), which could explain placental separation associated with NP. Also, NP was associated with downregulation of several placenta-regulatory genes (ABCG2, GCM1, EPAS1, and NR3C1), angiogenesis-related genes (VEGFA, FLT1, KDR, and ANGPT2), and glucose transporter coding genes (GLUT1, GLUT10, and GLUT12), as well as upregulation of hypoxia-related genes (HIF1A and EGLN3), which could elucidate placental insufficiency accompanying NP. In conclusion, our findings revealed for the first time, the key regulators and mechanisms underlying placental inflammation, separation, and insufficiency during NP, which might lead to the development of efficacious therapies or diagnostic aids by targeting the key molecular pathways.
Preventive interventions to reduce the burden of rheumatic heart disease in populations at risk: a systematic review protocol
Authors: Shimanda Panduleni Penipawa , Shumba Tonderai Washington , Brunström Mattias , Söderberg Stefan , Lindholm Lars , Iipinge Scholastika Ndatinda , Norström Fredrik
Published: July 2021

Background

Rheumatic heart disease is preventable, yet associated with significant health burden, mostly in low-resourced settings. It is prevalent among children and young adults living in impoverished areas. Primordial, primary, and secondary preventive measures have been recommended through health interventions and comprehensive programmes, although most implemented interventions are the high-resourced settings. The proposed review aims to synthesise the evidence of prevention effectiveness of implemented health interventions for the prevention of rheumatic heart disease.

Methods and design

This article describes a protocol for a systematic review. A predefined search strategy will be used to search for relevant literature published from the year 2000 to present. Electronic databases Medline, Web of Science, Scopus, and Cochrane Central Register of Controlled Trials will be searched for the studies, as well as reference lists of relevant studies included. Risk of bias and quality appraisal will be done for the included studies using ROBINS-I tool and Cochrane tool for assessing risk of bias in randomised control trials. Findings will be analysed in subgroups based on the level of intervention and prevention strategy implemented. We will present the findings in descriptive formats with tables and flow diagrams.

Discussion

This review will provide evidence on the prevention effectiveness of interventions or strategies implemented for the prevention of RHD. The findings of this will be significant for policy, practice, and research in countries planning to implement interventions.

Registration

PROSPERO ID: CRD42020170503.
Co-existence of Alport syndrome and C3 glomerulonephritis in a proband with family history
Authors: Ding Yin , Tang Xuanli , Du Yuanyuan , Chen Hongyu , Yu Dongrong , Zhu Bin , Yuan Bohan
Published: July 2021

Background

Alport syndrome and C3 glomerulonephritis (C3GN) are rare kidney diseases, frequently responsible for familial haematuria, proteinuria, and renal impairment. With the rapid development of molecular genetic testing, Alport syndrome causes have been restricted mostly to variants in the COL4A5 or COL4A3/COL4A4 genes. Moreover, a broad range of genetic contributors in the complement and complement-regulating proteins are definitely implicated in the pathogenesis of C3GN.

Methods

We sought a family with persistent microscopic haematuria associated with renal failure. Clinicopathologic and follow-up data were obtained, and molecular genetic testing was used to screen for pathogenic variants.

Results

We describe a three-generation family with Alport syndrome showing a dominant maternal inheritance. Notably, renal biopsy showed the concurrent histological evidence of C3GN in the proband harbouring an uncommon heterozygous variation in CFHR5, c.508G > A. The alteration leads to replacement of a highly conserved residue at position 170 of the β-strand subunit of CFHR5 (p.Val170Met). In silico analysis showed that the variation was predicted to deregulate complement activation by altering the structural properties and enhancing C3b binding capacity to compete with Complement Factor H (CFH), which was in line with experimental data previously published.

Conclusions

The comorbidity findings between Alport syndrome and C3GN indicate an underlying overlap and require further study.
COVID-19 pandemic in BRICS countries and its association with socio-economic and demographic characteristics, health vulnerability, resources, and policy response
Authors: Zhu Jingmin , Yan Wenxin , zhu Lin , Liu Jue
Published: July 2021

Background

Little attention has been paid to the comparison of COVID-19 pandemic responses and related factors in BRICS (Brazil, Russia, India, China, and South Africa) countries. We aimed at evaluating the association of daily new COVID-19 cases with socio-economic and demographic factors, health vulnerability, resources, and policy response in BRICS countries.

Methods

We conducted a cross-sectional study using data on the COVID-19 pandemic and other indicators of BRICS countries from February 26, 2020 to April 30, 2021. We compared COVID-19 epidemic in BRICS countries and analyzed related factors by log-linear Generalized Additive Model (GAM) models.

Results

In BRICS countries, India had the highest totally of confirmed cases with 18.76 million, followed by Brazil (14.45 million), Russia (4.81 million), and South Africa (1.58 million), while China (0.10 million) had the lowest figure. South Africa had the lowest rate of administered vaccine doses (0.18 million) among BRICS countries as of April 30, 2021. In the GAM model, a 1 unit increase in population density and policy stringency index was associated with a 5.17% and 1.95% growth in daily new COVID-19 cases (P < 0.001), respectively. Exposure–response curves for the effects of policy stringency index on daily new cases showed that there was a rapid surge in number of daily new COVID-19 cases when the index ranged from 0 to 45. The number of infections climbed slowly when the index ranged from 46 to 80, and decreased when the index was above 80 (P < 0.001). In addition, daily new COVID-19 cases (all P < 0.001) were also correlated with life expectancy at birth (-1.61%), extreme poverty (8.95%), human development index (-0.05%), GDP per capita (-0.18%), diabetes prevalence (0.66%), proportion of population aged 60 and above (2.23%), hospital beds per thousand people (-0.08%), proportion of people with access to improved drinking water (-7.40%), prevalence of open defecation (0.69%), and annual tourist/visitor arrivals (0.003%), after controlling other confounders. Different lag structures showed similar results in the sensitivity analysis.

Conclusions

Strong policy response is crucial to control the pandemic, such as effective containment and case management. Our findings also highlighted the importance of reducing socio-economic inequalities and strengthening the resilience of health systems to better respond to public health emergencies globally.

Graphic abstract
“Putting your own oxygen mask on first”: a qualitative study of siblings of adults with anorexia or bulimia
Authors: Karlstad Jannike , Moe Cathrine F. , Wattum Mari , Adelsten Stokland Ragni , Brinchmann Berit S.
Published: July 2021

Background

In families where one of the siblings has an eating disorder (ED), research indicates that the siblings without eating disorders (EDs) experience insufficient care and negative changes in family life. The illness then takes up a great deal of space within the family. Support from the siblings without EDs is considered to be important for the recovery of the sibling with ED. A key issue is how to involve siblings without EDs in treatment and establish what kind of support they themselves need. A majority of the research on EDs and family has focused on children and adolescents with EDs. The aim of this study is to expand knowledge about the experiences and coping strategies of sisters and brothers of adult women with anorexia nervosa or bulimia nervosa.

Methods

This qualitative study used a constructivist grounded theory approach. Individual semi-structured interviews were conducted with 10 sisters and brothers (aged 20–31 years). They were recruited from eating disorders and general psychiatrics units and from user organisations for patients with eating disorders within Norway. An iterative process of data collection, coding and analysis was employed in order to generate a theory about these participants’ experiences and strategies.

Results

The core category is “put your own oxygen mask on first”. It indicates that the siblings realize that they need to take care of themselves first, in order to be able to stay involved with their sister with the ED. The three subcategories; “taking a new role”, “distancing” and “adapted care” describe how the siblings handle their situation as the relatives of adult sisters with an ED. The siblings without ED became more responsible and independent and assumed a caregiving role. They downplayed their own needs to reduce their parents’ burden. This new role became difficult to fulfil and the siblings experienced that over time they needed more distance. Eventually, they developed a more manageable way of caring for their sister.

Conclusions

The findings suggest that the ED challenged the boundaries within the family. The siblings without ED assumed a caregiver role, gradually leading to exhaustion and the need to distance from the sister with the ED, to take care of themselves.
Seasonality impacts collective movements in a wild group-living bird
Authors: Papageorgiou Danai , Rozen-Rechels David , Nyaguthii Brendah , Farine Damien R.
Published: July 2021

Background

A challenge faced by animals living in groups with stable long-term membership is to effectively coordinate their actions and maintain cohesion. However, as seasonal conditions alter the distribution of resources across a landscape, they can change the priority of group members and require groups to adapt and respond collectively across changing contexts. Little is known about how stable group-living animals collectively modify their movement behaviour in response to environment changes, such as those induced by seasonality. Further, it remains unclear how environment-induced changes in group-level movement behaviours might scale up to affect population-level properties, such as a population’s footprint.

Methods

Here we studied the collective movement of each distinct social group in a population of vulturine guineafowl (Acryllium vulturinum), a largely terrestrial and non-territorial bird. We used high-resolution GPS tracking of group members over 22 months, combined with continuous time movement models, to capture how and where groups moved under varying conditions, driven by seasonality and drought.

Results

Groups used larger areas, travelled longer distances, and moved to new places more often during drier seasons, causing a three-fold increase in the area used at the population level when conditions turned to drought. By contrast, groups used smaller areas with more regular movements during wetter seasons.

Conclusions

The consistent changes in collective outcomes we observed in response to different environments raise questions about the role of collective behaviour in facilitating, or impeding, the capacity for individuals to respond to novel environmental conditions. As droughts will be occurring more often under climate change, some group living animals may have to respond to them by expressing dramatic shifts in their regular movement patterns. These shifts can have consequences on their ranging behaviours that can scale up to alter the footprints of animal populations.
Analytical investigation of the feather decoration technique of a seventeenth to eighteenth century Chinese imperial hanging screen
Authors: Gu An , Wang Na , Kong Yan-ju , Hou Jia-yu , Li Gen , Lei Yong
Published: July 2021

Decoration with feathers is a universal phenomenon in human history. Objects decorated with feathers were regarded as fine artworks and hence were enormously prized. In ancient China, dotting a surface with blue kingfisher feathers was a famous, complex and delicate decoration technique called diancui. Although various ancient diancui artworks appear in many museums around the world and researchers have realized significant results in studies of the history, technique and conservation of diancui, some key historical details are still not clear. In this research performed during restoration, an important object from the Palace Museum, the “Feather Decoration Hanging Screen with Birds and Flowers Pattern”, was analyzed by various scientific technologies. This object is a Chinese imperial artwork of the Qing dynasty (seventeenth to eighteenth century) decorated with kingfisher feathers and the feathers of several other birds, and it represents the highest level of this period. As a typical and valuable case, the results provide important clues for solving questions arising from related academic fields.
Coastal wetlands in Lianyungang, Jiangsu Province, China: probably the most important site globally for the Asian Dowitcher (Limnodromus semipalmatus)
Authors: Yang Ziyou , Li Jing , Han Yongxiang , Hassell Chris J. , Leung Kar-Sin Katherine , Melville David S. , Yu Yat-tung , Zhang Lin , Choi Chi-Yeung
Published: July 2021

Background

Despite an increasing number of surveys and a growing interest in birdwatching, the population and distribution of Asian Dowitcher (Limnodromus semipalmatus), a species endemic to the East Asian–Australasian and Central Asian Flyways, remains poorly understood, and published information about the species is largely outdated. In boreal spring 2019, over 22,432 Asian Dowitchers were recorded in a coastal wetland at Lianyungang, Jiangsu Province, China, constituting 97.5% of its estimated global population.

Methods

In 2019 and 2020, we conducted field surveys at Lianyungang to determine the numbers of Asian Dowitchers using the area during both southward and northward migrations. We also assessed the distribution and abundance of Asian Dowitchers elsewhere along the China coast by searching literature and consulting expert opinion.

Results

The coastal wetlands of Lianyungang are the most important stopover site for Asian Dowitchers during both northward and southward migrations; they supported over 90% of the estimated global population during northward migration in two consecutive years (May 2019 and 2020). This area also supported at least 15.83% and 28.42% (or 30.74% and 53.51% using modelled estimates) of the global population during southward migration in 2019 and 2020 respectively. Coastal wetlands in the west and north of Bohai Bay also have been important stopover sites for the species since the 1990s. Although comprehensive, long-term monitoring data are lacking, available evidence suggests that the population of the species may have declined.

Conclusions

The high concentration of Asian Dowitchers at Lianyungang during migration means the species is highly susceptible to human disturbances and natural stochastic events. The coastal wetlands of Lianyungang should be protected and potentially qualify for inclusion in China’s forthcoming nomination for World Heritage listing of Migratory Bird Sanctuaries along the Coast of Yellow Sea-Bohai Gulf of China (Phase II) in 2023. Additional research is needed to understand Asian Dowitchers’ distribution and ecology, as well as why such a high proportion of their population rely on the Lianyungang coast.
Changes in plant debris and carbon stocks across a subalpine forest successional series
Authors: Wang Zhihui , Zhao Lianjun , Bai Yi , Li Fei , Hou Jianfeng , Li Xuqing , Jiang Yurui , Deng Yuyue , Zheng Bingqian , Yang Wanqin
Published: July 2021

Background

As a structurally and functionally important component in forest ecosystems, plant debris plays a crucial role in the global carbon cycle. Although it is well known that plant debris stocks vary greatly with tree species composition, forest type, forest origin, and stand age, simultaneous investigation on the changes in woody and non-woody debris biomass and their carbon stock with forest succession has not been reported. Therefore, woody and non-woody debris and carbon stocks were investigated across a subalpine forest successional gradient in Wanglang National Nature Reserve on the eastern Qinghai-Tibet Plateau.

Results

Plant debris ranged from 25.19 to 82.89 Mg∙ha− 1 and showed a global increasing tendency across the subalpine forest successional series except for decreasing at the S4 successional stage. Accordingly, the ratios of woody to non-woody debris stocks ranged from 26.58 to 208.89, and the highest and lowest ratios of woody to non-woody debris stocks were respectively observed in mid-successional coniferous forest and shrub forest, implying that woody debris dominates the plant debris. In particular, the ratios of coarse to fine woody debris stocks varied greatly with the successional stage, and the highest and lowest ratios were found in later and earlier successional subalpine forests, respectively. Furthermore, the woody debris stock varied greatly with diameter size, and larger diameter woody debris dominated the plant debris. Correspondingly, the carbon stock of plant debris ranged from 10.30 to 38.87 Mg∙ha− 1 across the successional series, and the highest and lowest values were observed in the mid-coniferous stage and shrub forest stage, respectively. Most importantly, the carbon stored in coarse woody debris in later successional forests was four times higher than in earlier successional forests.

Conclusions

The stock and role of woody debris, particularly coarse woody debris, varied greatly with the forest successional stage and dominated the carbon cycle in the subalpine forest ecosystem. Thus, preserving coarse woody debris is a critical strategy for sustainable forest management.
Impact of learning through credit and value creation on the efficiency of Japanese commercial banks
Authors: Aduba Joseph , Izawa Hiroshi
Published: July 2021

This study investigates the learning curve of commercial banks regarding the efficiency of credit and value creation. However, current empirical methods for accessing the learning curve in organizations are not suitable for use in financial institutions. Considering bank-specific characteristics, weintroduce a dynamic learning curve using a cost function adjusted to capture learning-by-doing in banks.Using the model, we test several hypotheses on the impact of bank intermediary experience (learning) on the efficiency of credit and value creation in Japanese commercial banks. The findings show that bank intermediary learning significantly improves the cost efficiency gain in the gross value created, total credit created, and investment. However, bank intermediary experience has no significant effect on the efficiency of the economic value created for all the banks analyzed. These findings have practical implications for evaluating cost dynamics in bank credit and value creation, risk management, lending to the real sector, and shareholder value creation.
How can we categorise ‘nationality’ and ‘second generation’ in surveys without (re)producing stigmatisation?
Authors: Chimienti Milena , Guichard Eduardo , Bolzman Claudio , Le Goff Jean-Marie
Published: July 2021

Whilst reflexive migration studies have criticised the use of categories such as ‘nationality’ and ‘second generation’ in quantitative research, several gaps on how to develop such reflexivity remain. In qualitative data, the co-construction of knowledge seems feasible during fieldwork, whereas the deductive process of quantitative research limits such interactions and is more at risk of reproducing a ‘state thought’. Through a longitudinal database, the LIVES-FORS cohort survey of the National Center of Competence in Research LIVES – Overcoming Vulnerability: Life Course Perspectives and FORS – the Swiss Centre of Expertise in Social Sciences (herafter LCS), we engage in this discussion and provide some answers. The LCS is an annual longitudinal survey that, in 2013, started following a cohort of young adults born between 1988 and 1997 who grew up in Switzerland. The underlying hypothesis of the LCS is that migrants’ descendants have access to different resources (and often a lack thereof) to Swiss natives. In this paper, we discuss both the theoretical and empirical challenges to using the categories ‘nationality’ and ‘second generation’. We show the fluidity and subjectivity of these categories. By changing the definition of the category ‘second generation’, we increased the proportion of ‘second-generation’ participants from 43 to almost 62% of the sample. Looking across the five waves of the survey, we notice a 2% unexplained variation in the first nationality mentioned by the participants and 31% missing values regarding the nationality at birth – which are both indicators that nationality is a subjective category as well as a legal one. We illustrate that the static and neutral conceptions of these categories reproduce a false and stigmatised image of migrant descendants. To avoid these pitfalls we suggest developing multilevel geographical comparisons to consider the effects of time (age and historical), to use a wider range of information in order to be more precise, to examine different nationalities instead of focusing on the traditional nationalities of labour immigrants in a given country and to explore the reasons for the lack of answers to certain questions. Thus the questionnaires should include both more flexibility in the possibilities for answers and details and more-open questions regarding sensitive issues about the definition of the self. They should be developed through a participative and bottom-up process fostering mixed methods.
Impact of the Panama Canal expansion on Latin American and Caribbean ports: difference in difference (DID) method
Authors: Miller Kahuina , Hyodo Tetsuro
Published: July 2021

The expanded Panama Canal opened on June 26, 2016. This expansion is the third set of locks that enabled the canal to double its capacity through the addition of new traffic lanes, which allowed neo-Panamax and some post-Panamax vessels to transit across the canal. The widening of the canal has increased maritime traffic within Latin America and the Caribbean (LAC). Major ports in the regions have made huge investments in port expansion and infrastructural development to accommodate neo-Panamax vessels. In this study, we investigated the impact of the Panama Canal expansion (PCE) on the Latin America and the Caribbean (LAC) ports by using the Difference in Difference (DID) method. This impact was evaluated for 100 major and regular ports within the three sub-regions of LAC, namely Caribbean, Central, and South America, before and after the treatment effect, that is, the PCE. The findings from the model revealed that the average container port throughput (TEUs) for the treated ports (DTrp) was more than that of the controlled ports (CONTp) with transshipment hub, Central America, and South America having 20%, 12%, and 34% growth, respectively, since the PCE (the treatment) except for the Caribbean ports (DTrp), which experienced losses of 8% within the LAC region from 2010 to 2019.
Psychometric properties of the thyroid-specific quality of life questionnaire ThyPRO in Singaporean patients with Graves’ disease
Authors: Liew Huiling , Watt Torquil , Nan Luo , Tan Alvin W. K. , Chan Yiong Huak , Chew Daniel Ek Kwang , Dalan Rinkoo
Published: July 2021

Background

Graves’ disease is the most common cause of hyperthyroidism. It results in accelerated tissue metabolism with multi-organ involvement ranging from cardiovascular to neuropsychological function. This results in a negative impact on the quality of life (QOL) of the individual patient. We aim to evaluate the psychometric properties of ThyPRO, a Thyroid-related Patient Reported Outcome questionnaire, and validate its use in our multi-ethnic Asian patients with Graves’ hyperthyroidism.

Methods

Forty-seven consecutive Graves’ hyperthyroidism patients answered the ThyPRO questionnaire at baseline and at 4 months after treatment initiation. Data were recorded for thyroid related symptoms and signs, thyroid function tests and thyroid volume. We analyzed the internal consistency using Cronbach’s alpha, construct validity by evaluating relationship between clinical variables and ThyPRO scales, ceiling and floor effects, and responsiveness of ThyPRO to treatment based on Cohen’s effect size.

Results

Correlations between individual scale scores and free thyroxine concentrations were moderate and statistically significant: 0.21–0.64 (p <  0.05). There was high internal consistency between the items in this instrument, Cronbach’s alpha > 0.7 for all scales. ThyPRO was responsive to the changes in QOL after treatment (Effect Size: 0.20–0.77) in 9 of the 14 scales including the hyperthyroid symptoms and psychosocial scales (Tiredness, Cognitive complaints, Anxiety, Emotional susceptibility, Impact on Social, Daily and Sex life).

Conclusion

This study provides evidence that ThyPRO has satisfactory measurement properties in hyperthyroid Graves’ disease patients in Singapore population with the potential to complement clinical care.
Vibrational spectroscopy and chemometrics in GSR: review and current trend
Authors: Jain Bharti , Yadav Poonam
Published: July 2021

Background

This review represents many significant methods of chemometrics applied as data assessment methods originated by many hyphenated analytical techniques containing their application since its origin to today.

Main body of the abstract

The study has been divided into many parts, which contain many multivariate regression methods. The main aim of this study is to investigate the chemometrics tools used in GSR (gunshot residue) or forensic ballistics.

Short conclusion

As a final point, the end of part of this review deals with the applicability of chemometric methods in forensic ballistics. We select to give an elaborate description of many significant tools established with their algorithm in admire of utilizing and accepting them by researchers not very aware with chemometrics.