bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Regulation of Heme Oxygenase-1 Expression by E2F Transcription Factor 2 in Lung Fibroblast Cells: Relevance to Idiopathic Pulmonary Fibrosis
Authors: Ye Qinmao , Taleb Sarah J. , Wang Heather , Parinandi Narasimham L. , Kass Daniel J. , Rojas Mauricio , Wang Cankun , Ma Qin , Zhao Jing , Zhao Yutong
Published: October 2022

Idiopathic pulmonary fibrosis (IPF) is a fatal chronic lung disease. Heme oxygenase-1 (HMOX1/HO-1) is an enzyme that catalyzes the degradation of heme. The role of HO-1 in the pathogenesis of IPF has been studied; however, the molecular regulation of HO-1 and its role in IPF are still unclear. In this study, we found that HO-1 protein levels significantly increased in lung myofibroblasts in IPF patients and in lungs in a murine model of bleomycin-induced lung fibrosis. In addition, we observed that administration of a E2F transcription factor inhibitor elevated HO-1 mRNA and protein levels in lung fibroblasts. Downregulation of E2F2 by siRNA transfection increased HO-1 mRNA and protein levels, while overexpression of E2F2 reduced HO-1 levels. However, overexpression of E2F2 did not alter hemin-induced HO-1 protein levels. Furthermore, modulation of HO-1 levels regulated TGF-β1-induced myofibroblast differentiation without altering the phosphorylation of Smad2/3 in lung fibroblast cells. Moreover, the phosphorylation of protein kinase B (Akt) was significantly upregulated in HO-1-depleted lung fibroblast cells. In summary, this study demonstrated that E2F2 regulates the baseline expression of HO-1, but has no effect on modulating HO-1 expression by hemin. Finally, elevated HO-1 expression contributes to the TGF-β1-induced lung myofibroblast differentiation through the activation of the serine/threonine kinase AKT pathway. Overall, our findings suggest that targeting E2F2/HO-1 might be a new therapeutic strategy to treat fibrotic diseases such as IPF.
Modelling Hyperglycaemia in an Epithelial Membrane Model: Biophysical Characterisation
Authors: Reis Ana , Teixeira Joana P. F. , Silva Ana M. G. , Ferreira Mariana , Gameiro Paula , de Freitas Victor
Published: October 2022

Biomimetic models are valuable platforms to improve our knowledge on the molecular mechanisms governing membrane-driven processes in (patho)physiological conditions, including membrane permeability, transport, and fusion. However, current membrane models are over simplistic and do not include the membrane’s lipid remodelling in response to extracellular stimuli. Our study describes the synthesis of glycated dimyristoyl-phosphatidylethanolamine (DMPE-glyc), which was structurally characterised by mass spectrometry (ESI-MS) and quantified by NMR spectroscopy to be further incorporated in a complex phospholipid (PL) membrane model enriched in cholesterol (Chol) and (glyco)sphingolipids (GSL) designed to mimic epithelial membranes (PL/Chol/GSL) under hyperglycaemia conditions. Characterisation of synthesised DMPE-glyc adducts by tandem mass spectrometry (ESI-MS/MS) show that synthetic DMPE-glyc adducts correspond to Amadori products and quantification by 1H NMR spectroscopy show that the yield of glycation reaction was 8%. The biophysical characterisation of the epithelial membrane model shows that excess glucose alters the thermotropic behaviour and fluidity of epithelial membrane models likely to impact permeability of solutes. The epithelial membrane models developed to mimic normo- and hyperglycaemic scenarios are the basis to investigate (poly)phenol-lipid and drug–membrane interactions crucial in nutrition, pharmaceutics, structural biochemistry, and medicinal chemistry.
Effects of Psychotropic Medication on Somatic Sterol Biosynthesis of Adult Mice
Authors: Balog Marta , Anderson Allison C , Heffer Marija , Korade Zeljka , Mirnics Karoly
Published: October 2022

Polypharmacy is commonly used to treat psychiatric disorders. These combinations often include drugs with sterol biosynthesis inhibiting side effects, including the antipsychotic aripiprazole (ARI), and antidepressant trazodone (TRZ). As the effects of psychotropic medications are poorly understood across the various tissue types to date, we investigated the effects of ARI, TRZ, and ARI + TRZ polypharmacy on the post-lanosterol biosynthesis in three cell lines (Neuro2a, HepG2, and human dermal fibroblasts) and seven peripheral tissues of an adult mouse model. We found that both ARI and TRZ strongly interfere with the function of 7-dehydrocholesterol reductase enzyme (DHCR7) and lead to robust elevation in 7-dehydrocholesterol levels (7-DHC) and reduction in desmosterol (DES) across all cell lines and somatic tissues. ARI + TRZ co-administration resulted in summative or synergistic effects across the utilized in vitro and in vivo models. These findings suggest that at least some of the side effects of ARI and TRZ are not receptor mediated but arise from inhibiting DHCR7 enzyme activity. We propose that interference with sterol biosynthesis, particularly in the case of simultaneous utilization of medications with such side effects, can potentially interfere with functioning or development of multiple organ systems, warranting further investigation.
Aortic Aneurysm and Dissection: Heterogeneity and Molecular Mechanisms
Authors: Lu Hong S. , Sawada Hisashi , Wu Congqing
Published: October 2022

Aortic aneurysms and dissections (AAD) are devastating aortic diseases with high risks for aortic rupture, leading to uncontrolled bleeding and death [...]
Clinical Applications of Mixed Reality and 3D Printing in Congenital Heart Disease
Authors: Lau Ivan , Gupta Ashu , Ihdayhid Abdul , Sun Zhonghua
Published: October 2022

Understanding the anatomical features and generation of realistic three-dimensional (3D) visualization of congenital heart disease (CHD) is always challenging due to the complexity and wide spectrum of CHD. Emerging technologies, including 3D printing and mixed reality (MR), have the potential to overcome these limitations based on 2D and 3D reconstructions of the standard DICOM (Digital Imaging and Communications in Medicine) images. However, very little research has been conducted with regard to the clinical value of these two novel technologies in CHD. This study aims to investigate the usefulness and clinical value of MR and 3D printing in assisting diagnosis, medical education, pre-operative planning, and intraoperative guidance of CHD surgeries through evaluations from a group of cardiac specialists and physicians. Two cardiac computed tomography angiography scans that demonstrate CHD of different complexities (atrial septal defect and double outlet right ventricle) were selected and converted into 3D-printed heart models (3DPHM) and MR models. Thirty-four cardiac specialists and physicians were recruited. The results showed that the MR models were ranked as the best modality amongst the three, and were significantly better than DICOM images in demonstrating complex CHD lesions (mean difference (MD) = 0.76, p = 0.01), in enhancing depth perception (MD = 1.09, p = 0.00), in portraying spatial relationship between cardiac structures (MD = 1.15, p = 0.00), as a learning tool of the pathology (MD = 0.91, p = 0.00), and in facilitating pre-operative planning (MD = 0.87, p = 0.02). The 3DPHM were ranked as the best modality and significantly better than DICOM images in facilitating communication with patients (MD = 0.99, p = 0.00). In conclusion, both MR models and 3DPHM have their own strengths in different aspects, and they are superior to standard DICOM images in the visualization and management of CHD.
Surgical Management of Synucleinopathies
Authors: Sriram Sai , Root Kevin , Chacko Kevin , Patel Aashay , Lucke-Wold Brandon
Published: October 2022

Synucleinopathies represent a diverse set of pathologies with significant morbidity and mortality. In this review, we highlight the surgical management of three synucleinopathies: Parkinson’s disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA). After examining underlying molecular mechanisms and the medical management of these diseases, we explore the role of deep brain stimulation (DBS) in the treatment of synuclein pathophysiology. Further, we examine the utility of focused ultrasound (FUS) in the treatment of synucleinopathies such as PD, including its role in blood–brain barrier (BBB) opening for the delivery of novel drug therapeutics and gene therapy vectors. We also discuss other recent advances in the surgical management of MSA and DLB. Together, we give a diverse overview of current techniques in the neurosurgical management of these pathologies.
Retinal Microvascular Abnormalities and Systemic Arterial Stiffness Are the First Manifestation of Cardiovascular Abnormalities in Patients with Untreated Moderate to Severe Obstructive Sleep Apnoea and with Low to Intermediate Cardiovascular Risk—A Pilot Study
Authors: Seshadri Swathi , Shokr Hala , Gherghel Doina
Published: October 2022

This study aimed to investigate macro- and microvascular function parameters and their relationship with known markers of cardiovascular risk in patients with untreated moderate to severe obstructive sleep apnoea (OSA). Fourteen patients with moderate to severe OSA and fourteen controls were included in the present study. General assessments included BMI, systemic blood pressure (BP) and circulating markers for oxidative stress and endothelial function. Additional assessments included 24 h BP and heart rate monitoring, as well as the assessment of heart rate variability. Macro- and microvascular assessments included augmentation index, carotid intima-media thickness, brachial artery flow-mediated dilation, as well as various retinal microvascular function assessments, using the Dynamic Retinal Vessel Analyzer. All participants completed the Short Form Health Survey, Functional Outcomes of Sleep Questionnaire, and Epworth Sleepiness Scale. The results show that, in comparison to controls, BMI (p = 0.003) and AIx (p = 0.025) were significantly higher in the OSA group. There was, however, no significant difference between groups with regard to other measured systemic general, vascular and circulatory parameters (all p > 0.05). Nevertheless, the retinal microvascular function showed various alterations in the OSA patients, including a delayed reaction time in response to flicker (p = 0.047), as well as a decreased dilation amplitude (p = 0.004), dilation slope (p = 0.004), and post-flicker constriction (p = 0.015). In addition, the observed SlopeAD alterations correlated negatively with BMI values only in the OSA group (r = −0.46, p = 0.045). In conclusion, individuals with untreated moderate to severe OSA but without overt CVD, exhibit signs of increased arterial stiffness and retinal microvascular dysfunction, which can be early indicators for future vascular complications.
The Endometriotic Neoplasm Algorithm for Risk Assessment (e-NARA) Index Sheds Light on the Discrimination of Endometriosis-Associated Ovarian Cancer from Ovarian Endometrioma
Authors: Kawahara Naoki , Kawaguchi Ryuji , Maehana Tomoka , Yamanaka Shoichiro , Yamada Yuki , Kobayashi Hiroshi , Kimura Fuminori
Published: October 2022

Background: Magnetic resonance (MR) relaxometry provides a noninvasive tool to discriminate endometriosis-associated ovarian cancer (EAOC) from ovarian endometrioma (OE) with high accuracy. However, this method has a limitation in discriminating malignancy in clinical use because the R2 value depends on the device manufacturer and repeated imaging is unrealistic. The current study aimed to reassess the diagnostic accuracy of MR relaxometry and investigate a more powerful tool to distinguish EAOC from OE. Methods: This retrospective study was conducted at our institution from December, 2012, to May, 2022. A total of 150 patients were included in this study. Patients with benign ovarian tumors (n = 108) mainly received laparoscopic surgery, and cases with suspected malignancy (n = 42) underwent laparotomy. Information from a chart review of the patients’ medical records was collected. Results: A multiple regression analysis revealed that the age, the tumor diameter, and the R2 value were independent malignant predicting factors. The endometriotic neoplasm algorithm for risk assessment (e-NARA) index provided high accuracy (sensitivity, 85.7%; specificity, 87.0%) to discriminate EAOC from OE. Conclusions: The e-NARA index is a reliable tool to assess the probability of malignant transformation of endometrioma.
Special Issue “Role of STAT3 in Oncogenesis”
Authors: Ahn Kwang Seok
Published: October 2022

This Biomedicines Special Issue was designed to attract articles that focused on the pleiotropic role of the signal transducer and activator of transcription 3 (STAT3) transcription factor in different facets of tumorigenesis [...]
Convergent Evolution of Adhesive Properties in Leaf Insect Eggs and Plant Seeds: Cross-Kingdom Bioinspiration
Authors: Büscher Thies H. , Gorb Stanislav N.
Published: October 2022

Plants and animals are often used as a source for inspiration in biomimetic engineering. However, stronger engagement of biologists is often required in the field of biomimetics. The actual strength of using biological systems as a source of inspiration for human problem solving does not lie in a perfect copy of a single system but in the extraction of core principles from similarly functioning systems that have convergently solved the same problem in their evolution. Adhesive systems are an example of such convergent traits that independently evolved in different organisms. We herein compare two analogous adhesive systems, one from plants seeds and one from insect eggs, to test their properties and functional principles for differences and similarities in order to evaluate the input that can be potentially used for biomimetics. Although strikingly similar, the eggs of the leaf insect Phyllium philippinicum and the seeds of the ivy gourd Coccinia grandis make use of different surface structures for the generation of adhesion. Both employ a water-soluble glue that is spread on the surface via reinforcing fibrous surface structures, but the morphology of these structures is different. In addition to microscopic analysis of the two adhesive systems, we mechanically measured the actual adhesion generated by both systems to quantitatively compare their functional differences on various standardized substrates. We found that seeds can generate much stronger adhesion in some cases but overall provided less reliable adherence in comparison to eggs. Furthermore, eggs performed better regarding repetitive attachment. The similarities of these systems, and their differences resulting from their different purposes and different structural/chemical features, can be informative for engineers working on technical adhesive systems.
Investigation on the Anisotropic Wetting Properties of Water Droplets on Bio-Inspired Groove Structures Fabricated by 3D Printing and Surface Modifications
Authors: Mai Ngoc Phuong Uyen , Chen Po-Yu
Published: October 2022

The self-driving structure to orientate the water movement has attracted considerable attention. Inspired by the wedgelike structures of biological materials in nature, such as spider silks and cactus spines, anisotropic spreading can be realized by combining Laplace pressure gradient and hydrophilic surface. In this study, a series of groove patterns were fabricated by a combination of 3D printing and surface modification. PLA pattern was modified by the atmospheric pressure plasma, followed by grafting with hydrolyzed APTES. This work reports the anisotropic transport of water droplets on a series of designed dart-shaped groove patterns with specific angles in the main arrow and tail regions. This structure can induce capillary force to regulate droplets from the main cone to two wedgelike, whereas the droplets are hindered toward the opposite side is oat the vicinity of the groove’s tail. By means of the experiment, the mechanism of water transport in this pattern was revealed. This study can contribute a potential approach to manipulate and apply anisotropic wetting in many fields.
Trace Determination of Grouper Nervous Necrosis Virus in Contaminated Larvae and Pond Water Samples Using Label-Free Fiber Optic Nanoplasmonic Biosensor
Authors: Chen Yuan-Yu , Wu Chih-Lu , Hsu Chia-Wei , Wang Chih-Hui , Su Chung-Rui , Huang Chun-Jen , Chen Hau-Ren , Chau Lai-Kwan , Wang Shau-Chun
Published: October 2022

We developed a fast (<20 min), label-free fiber optic particle plasmon resonance (FOPPR) immunosensing method to detect nervous necrosis virus (NNV), which often infects high-value economic aquatic species, such as grouper. Using spiked NNV particles in a phosphate buffer as samples, the standard calibration curve obtained was linear (R2 = 0.99) and the limit of detection (LOD) achieved was 2.75 × 104 TCID50/mL, which is superior to that obtained using enzyme-linked immunosorbent assay (ELISA). By using an enhancement method called fiber optic nanogold-linked immunosorbent assay (FONLISA), the LOD can be further improved to <1 TCID50/mL, which is comparable to that found by the conventional qPCR method. Employing the larvae homogenate samples of NNV-infected grouper, the results obtained by the FOPPR biosensor agree with those obtained by the quantitative polymerase chain reaction (qPCR) method. We also examined pond water samples from an infected container in an indoor aquaculture facility. The lowest detectable level of NNV coat protein was found to be 0.17 μg/mL, which is one order lower than the LOD reported by ELISA. Therefore, we demonstrated the potential of the FOPPR biosensor as an outbreak surveillance tool, which is able to give warning indication even when the trend of larvae death toll increment is still not clear.
A Novel Activated Biochar-Based Immunosensor for Rapid Detection of E. coli O157:H7
Authors: Sobhan Abdus , Jia Fei , Kelso Lisa Cooney , Biswas Sonatan Kumar , Muthukumarappan Kasiviswanathan , Cao Changyong , Wei Lin , Li Yanbin
Published: October 2022

E. coli O157:H7, one of the major foodborne pathogens, can cause a significant threat to the safety of foods. The aim of this research is to develop an activated biochar-based immunosensor that can rapidly detect E. coli O157:H7 cells without incubation in pure culture. Biochar was developed from corn stalks using proprietary reactors and then activated using steam-activation treatment. The developed activated biochar presented an enhanced surface area of 830.78 m2/g. To develop the biosensor, the gold electrode of the sensor was first coated with activated biochar and then functionalized with streptavidin as a linker and further immobilized with biotin-labeled anti-E. coli polyclonal antibodies (pAbs). The optimum concentration of activated biochar for sensor development was determined to be 20 mg/mL. Binding of anti-E. coli pAbs with E. coli O157:H7 resulted in a significant increase in impedance amplitude from 3.5 to 8.5 kΩ when compared to an only activated biochar-coated electrode. The developed immunosensor was able to detect E. coli O157:H7 cells with a limit of detection of 4 log CFU/mL without incubation. Successful binding of E. coli O157:H7 onto an activated biochar-based immunosensor was observed on the microelectrode surface in scanning electron microscopy (SEM) images.
Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent Detection
Authors: Sompark Chalermwoot , Chawjiraphan Wireeya , Sukmak Manatsaphon , Cha’on Ubon , Anutrakulchai Sirirat , Pongprayoon Prapasiri , Putnin Thitirat , Pimalai Dechnarong , Pinrod Visarute , Japrung Deanpen
Published: October 2022

The instability of human serum albumin (HSA) in urine samples makes fresh urine a requirement for microalbumin analyses using immunoturbidimetry. Here, we determined the ability of an aptasensor-based fluorescent platform to detect microalbumin in old, boric acid-preserved urine samples. Our results show that the cleavage site of protease enzymes on urine albumin protein differed from the binding position of the aptamer on HSA protein, suggesting the aptasensor may be effective for albumin detection in non-fresh urine. Furthermore, the addition of boric acid in urine samples over a short term (at ambient temperature (Ta) and 4 °C), long term (−20 and −80 °C), and following freeze–thawing (1–3 cycles) did not significantly affect albumin stability, as analyzed using the aptasensor. Therefore, boric acid stabilized has in urine stored over a short- and long-term. Thus, the aptasensor developed by us is applicable for HSA detection in boric acid-preserved urine that has been stored for 7-d at Ta and 4 °C, and in the long-term at −80 °C.
Compensation Mechanisms May Not Always Account for Enhanced Multisensory Illusion in Older Adults: Evidence from Sound-Induced Flash Illusion
Authors: Zhou Heng , Liu Xiaole , Yu Junming , Yue Chunlin , Wang Aijun , Zhang Ming
Published: October 2022

Sound-induced flash illusion (SiFI) is typical auditory dominance phenomenon in multisensory illusion. Although a number of studies have explored the SiFI in terms of age-related effects, the reasons for the enhanced SiFI in older adults are still controversial. In the present study, older and younger adults with equal visual discrimination were selected to explore age differences in SiFI effects, and to explore the neural indicators by resting-state functional magnetic resonance imaging (rs-fMRI) signals. A correlation analysis was calculated to examine the relationship between regional homogeneity (ReHo) and the SiFI. The results showed that both younger and older adults experienced significant fission and fusion illusions, and fission illusions of older adults were greater than that of younger adults. In addition, our results showed ReHo values of the left middle frontal gyrus (MFG), the right inferior frontal gyrus (IFG) and right superior frontal gyrus (SFG) were significantly positively correlated with the SiFI in older adults. More importantly, the comparison between older and younger adults showed that ReHo values of the right superior temporal gyrus (STG) decreased in older adults, and this was independent of the SiFI. The results indicated that when there was no difference in unisensory ability, the enhancement of multisensory illusion in older adults may not always be explained by compensation mechanisms.
Activation Patterns of Functional Brain Network in Response to Action Observation-Induced and Non-Induced Motor Imagery of Swallowing: A Pilot Study
Authors: Xiong Hao , Chen Jin-Jin , Gikaro John M. , Wang Chen-Guang , Lin Feng
Published: October 2022

Action observation (AO) combined with motor imagery (MI) was verified as more effective in improving limb function than AO or MI alone, while the underlying mechanism of swallowing was ambiguous. The study aimed at exploring the efficacy of AO combined with MI in swallowing. In this study, twelve subjects performed the motor imagery of swallowing (MI-SW) during magnetoencephalography (MEG) scanning, and trials were divided into three groups: the non-induced group (control group, CG), male AO-induced group (M-AIG), and female AO-induced group (F-AIG). We used event-related spectral perturbations (ERSPs) and phase locking value (PLV) to assess the degree of activation and connectivity of the brain regions during MI-SW in the three groups. The results showed that compared to CG, F-AIG and M-AIG significantly activated more brain regions in the frontoparietal, attention, visual, and cinguloopercular systems. In addition, M-AIG significantly activated the sensorimotor cortex compared to CG and F-AIG. For the brain network, F-AIG and M-AIG increased the diffusion of non-hub hot spots and cold hubs to the bilateral hemispheres which enhanced interhemispheric functional connectivity and information transmission efficiency in the MI-SW task. This study provided supporting evidence that AO induction could enhance the effect of MI-SW and supported the application of AO-induced MI-SW in clinical rehabilitation.
On the Embodiment of Social Cognition Skills: The Inner and Outer Body Processing Differently Contributes to the Affective and Cognitive Theory of Mind
Authors: Canino Silvia , Raimo Simona , Boccia Maddalena , Di Vita Antonella , Palermo Liana
Published: October 2022

A specific interpretation of embodiment assigns a central role to the body representations (BR) in cognition. In the social cognition domain, BR could be pivotal in representing others’ actions and states. However, empirical evidence on the relationship between different BR and social cognition, in terms of Theory of Mind (ToM), in the same sample of participants is missing. Here, this relationship was explored considering individual differences in the action-oriented BR (aBR), nonaction-oriented BR (NaBR), and subjective predisposition toward internal bodily sensations (interoceptive sensibility, ISe). Eighty-two healthy adults were given behavioral measures probing aBR, NaBR, ISe, and affective/cognitive ToM. The results suggest that NaBR, which mainly relies on exteroceptive signals, predicts individual differences in cognitive ToM, possibly because it can allow differentiating between the self and others. Instead, the negative association between affective ToM and ISe suggests that an alteration of the internal body state representation (i.e., over-reporting interoceptive sensations) can affect emotional processing in social contexts. The finding that distinct aspects of the body processing from within (ISe) and from the outside (NaBR) differently contribute to ToM provides empirical support to the BR role in social cognition and can be relevant for developing interventions in clinical settings.
Attachment and Depressive Symptoms in Adolescence: The Mediatory Role of Emotion Awareness
Authors: Carapeto Maria João , Domingos Raquel , Veiga Guida
Published: October 2022

Attachment seems to influence depression through emotion regulation. However, no study has yet examined the mediatory role of emotion awareness, a particular subset of emotion regulation abilities, in the relationship between attachment and depressive problems in early and middle adolescence. The aim of this study is to examine the direct and indirect effects of attachment on depressive symptoms in adolescence, considering the mediatory role of emotion awareness dimensions. A sample of adolescents (n = 223) filled up self-report questionnaires on attachment, emotion awareness and depression. Serial mediation models suggest direct effects on depression: negative for secure attachment and positive for anxious/ambivalent attachment. Anxious/ambivalent attachment has a positive indirect effect through lower differentiation of emotions. Both secure and anxious/ambivalent attachment have indirect positive effects on depression through the sequence of bodily unawareness and differentiation of emotions. Differentiating emotions has a central role in mediating the relationship between attachment and depressive symptoms, and the lack of bodily awareness of emotions contributes to such mediation.
Exploring the Mechanisms Linking Transformational Leadership, Perceived Organizational Support, Creativity, and Performance in Hospitality: The Mediating Role of Affective Organizational Commitment
Authors: Wang Chung-Jen
Published: October 2022

Integrating leadership and creativity theories, this study proposed and examined a model linking transformational leadership, perceived organizational support, affective organizational commitment, creativity, and performance. Structural equation modeling was thus performed using 362 employee–supervisor dyads from the international tourist hotels in Taiwan. The results indicated that both transformational leadership and perceived organizational support were significantly and positively related to employee affective organizational commitment, creativity, and performance, while affective organizational commitment had significantly positive influences on creativity and performance. Support was also found for the significant and positive mediating relationships between affective organizational commitment and the influences of both transformational leadership and perceived organizational support on creativity and performance. The theoretical and practical implications of these findings for hospitality are also discussed.
The Light and Dark Sides of Student Engagement: Profiles and Their Association with Perceived Autonomy Support
Authors: Yang Dong , Cai Zhenyu , Tan Yu , Zhang Chen , Li Mengti , Fei Cheng , Huang Ronghuai
Published: October 2022

School engagement has assumed an important place in current developmental psychology and educational research due to its potential to address students’ low achievement, high dropout rates, and misbehavior. Although much has been written about the antecedents and outcomes of student engagement, literature on how students’ level of engagement differs in response to different teaching styles was missing on a large scale. Understanding the patterns and risks linked with student engagement provides opportunities for targeted intervention. This study explored primary school students’ engagement and burnout profiles and how different profiles interacted with perceived classroom teaching styles (i.e., autonomy-supportive & autonomy suppressive). Latent profile analysis resulted in four student engagement subgroups: moderately engaged, engaged, moderately burned out, and burned out. Students clustered into engagement groups were likely to report higher autonomy support from teachers. In contrast, burned-out groups were more likely to rate teachers’ teaching styles as suppressive (i.e., autonomy suppressive). Collectively, the study indicated that autonomy-supportive teaching behaviors are pivotal in understanding student engagement and school burnout. Thus, tailored teacher-focused intervention programs that enhance teachers’ awareness of autonomy-supportive teaching is important. The significance of the findings with the demand-resource model (in the education context) was discussed.
A Post-Analysis of the Introduction of the EU Directive 92/57/EEC in the Swedish Construction Industry
Authors: Berglund Leif , Johansson Jan , Johansson Maria , Nygren Magnus , Samuelson Björn , Stenberg Magnus
Published: October 2022

The EU directive 92/57/EEC focuses on ensuring that health and safety-related matters are taken into consideration during every stage of construction-related work and has been introduced into the regulations of the member countries. In 2006, Sweden was tasked by the European Commission to clarify its implementation of the directive, including which management roles and responsibilities were to come into effect during both the planning and eventual execution of construction work—changes that ultimately were introduced into the national regulations in 2009. Focusing on the accident trends in the construction industry in the years immediately following these regulatory changes, we find that the new management roles and responsibilities had no apparent effect on the accident rates. Furthermore, we argue that there is a need to broaden the analysis regarding the implementation of the EU directive 92/57/EEC to also include nation-specific changes to health and safety management and policy. These qualitative studies should also include a dedicated focus on how changes to management structures and processes may affect the prevalence of occupational diseases specifically.
Use of Processed Sugarcane Bagasse Ash in Concrete as Partial Replacement of Cement: Mechanical and Durability Properties
Authors: Memon Shazim Ali , Javed Usman , Shah Muhammad Izhar , Hanif Asad
Published: October 2022

Using biomass waste as supplementary cementing material (SCM) in concrete has attracted researchers’ attention for efficient waste utilization and reducing cement demand. Sugarcane bagasse ash (SCBA) is one such example of biomass waste. It is an agricultural waste obtained when sugarcane bagasse from the sugar industry is used for power generation and disposed of in open-air dumping sites. Its waste disposal causes the generation of particulate matter, degrading air quality. In this study, the effect of processed SCBA as SCM in concrete has been investigated. The processing of the SCBA involved the removal of fibrous and carbon-containing particles by sieving followed by grinding. The SCBA was ground for 45 min until the surface area was comparable to that of cement and was then used for further characterization and incorporation into concrete. The 45 min grinding time resulted in 2.92 times higher pozzolanic reactivity of the SCBA. The SCBA was incorporated by replacing cement in different weight fractions (10%, 20%, 30%, 40%) in concrete. Test results showed that the concrete workability increased with SCBA incorporation, whereas the resulting concrete density was reduced. The results of the mechanical properties, including compressive sstrength and hardened density, were enhanced upon the cement replacement by SCBA. Concrete containing 30% SCBA can be used for structural applications as its 28 days compressive strength was 21 MPa, which complies with ACI 318-16 specifications. Concrete resistance against scaling and leaching due to adverse effects of sulfuric and hydrochloric acid considerably increased with SCBA addition and was due to microstructure densification by secondary hydrates formation as lower portlandite content was detected by thermogravimetric analysis. Hence, SCBA processing increases its reactivity, as reflected by the improved mechanical properties and greater durability of SCBA-incorporated concrete.
Deep Learning-Enriched Stress Level Identification of Pretensioned Rods via Guided Wave Approaches
Authors: Zhang Zi , Tang Fujian , Cao Qi , Pan Hong , Wang Xingyu , Lin Zhibin
Published: October 2022

By introducing pre-compression/inverse moment through prestressing tendons or rods, prestressed concrete (PC) structures could overcome conventional concrete weakness in tension, and thus, these tendons or rods are widely accepted in a variety of large-scale, long-span structures. Unfortunately, prestressing tendons or rods embedded in concrete are vulnerable to degradation due to corrosion. These embedded members are mostly inaccessible for visual or direct destructive assessments, posing challenges in determining the prestressing level and any corrosion-induced damage. As such, ultrasonic guided waves, as one of the non-destructive examination methods, could provide a solution to monitor and assess the health state of embedded prestressing tendons or rods. The complexity of the guided wave propagation and scattering in nature, as well as high variances stemming from the structural uncertainty and noise interference PC structures may experience under complicated operational and harsh environmental conditions, often make traditional physics-based methods invalid. Alternatively, the emerging machine learning approaches have potential for processing the guided wave signals with better capability of decoding structural uncertainty and noise. Therefore, this study aimed to tackle stress level prediction and the rod embedded conditions of prestressed rods in PC structures through guided waves. A deep learning approach, convolutional neural network (CNN), was used to process the guided wave dataset. CNN-based prestress level prediction and embedding condition identification of rods were established by the ultrasonic guided wave technique. A total of fifteen scenarios were designed to address the effectiveness of the stress level prediction under different noise levels and grout materials. The results demonstrate that the deep learning approaches exhibited high accuracy for prestressing level prediction under structural uncertainty due to the varying surrounding grout materials. With different grout materials, accuracy could reach up to 100% under the noise level of 90 dB, and still maintain the acceptable range of 75% when the noise level was as high as 70 dB. Moreover, the t-distributed stochastic neighbor embedding technology was utilized to visualize the feature maps obtained by the CNN and illustrated the correlation among different categories. The results also revealed that the proposed CNN model exhibited robustness with high accuracy for processing the data even under high noise interference.
Study on Mechanical Properties of Heap Deposited Fly Ash Based Geopolymers with Different Alkaline Activator Properties
Authors: Sisol Martin , Marcin Michal , Dvořák Karel , Suďová Michaela , Ivanková Viera
Published: October 2022

Geopolymers can improve environmental protection and are therefore considered a 21st-century material that requires special attention. The main objective of this study was to examine the utilization of fly ash deposited on a heap as a potential raw material for the geopolymerization process. In this work, flexural and compressive strengths, as well as water absorption of geopolymers, were evaluated based on alkaline activator properties. Na2O content, SiO2/Na2O molar ratio and fly ash-to-water ratio were key observed factors. Mechanical strengths tests were conducted after 7, 28, 90, 180, and 360 days. The research findings showed that geopolymer cured at appropriate conditions and with a suitable alkaline activator can reach a compressive strength of more than 55 MPa. The presented study highlighted the possibility of the efficient use of fly ash to produce useful materials, i.e., geopolymers, which contribute to environmentally friendly solutions. This approach is fully in line with the principles of sustainable development.
Exploring the Knowledge Domain of Risk Management in Prefabricated Construction
Authors: Li Tianxin , Li Zhongfu , Li Long , Jiang Peng
Published: October 2022

Risks hinder the role of prefabricated construction (PC) in promoting construction automation. Although existing research has focused on risk management (RM) in PC, the lack of a global perspective has affected the effectiveness of RM. Accordingly, this paper adopts a scientometric analysis to review the knowledge domain of RM in PC. A total of 144 articles were selected from the Scopus database for journal citation analysis, document co-citation analysis, and keyword co-occurrence analysis. The results show that since 2011, the annual publications show an overall upward trend. International Journal of Construction Management, Journal of Building Engineering, and Buildings have been cited more frequently recently. PC research, RM research, environmental sustainability research, and ergonomic research provide a solid foundation for the research on RM in PC. Existing studies are conducted from five knowledge themes, namely, Supply chain and industry, Decision and optimization, Safety and health, Environment and overheating, and Investment and cost. Further, current research content, future research needs, and RM strategies for PC practices are discussed. This study helps stimulate further promising research and enhance the effectiveness of RM in PC in practice.
Effect of Light and Heavy Rare Earth Doping on the Physical Structure of Bi2O2CO3 and Their Performance in Photocatalytic Degradation of Dimethyl Phthalate
Authors: He Qingyun , Liu Xingqiang , Li Feng , Li Fang , Tao Leiming , Yu Changlin
Published: October 2022

In order to solve the problem of environmental health hazards caused by phthalate esters, a series of pure Bi2O2CO3 and light (La, Ce, Pr, Nd, Sm and Eu) and heavy (Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu) rare earth-doped Bi2O2CO3 samples were prepared by hydrothermal method. The crystalline phase composition and physical structure of the samples calcined at 300 °C were studied, and we found that the rare earth ion doping promoted the transformation of Bi2O2CO3 to β-Bi2O3 crystalline phase, thus obtaining a mixed crystal phase photocatalyst constituted by rare earth-ion-doped Bi2O2CO3/β-Bi2O3. The Bi2O3/Bi2O2CO3 heterostructure had a lower band gap and more efficient charge transfer. The fabricated samples were applied to the photocatalytic degradation of dimethyl phthalate (DMP) under a 300 W tungsten lamp, and it was found that the rare earth ion doping enhanced the photocatalytic degradation activity of DMP, in which the heavy rare earth of Er-doped sample reached 78% degradation for DMP at 150 min of light illumination. In addition, the doping of rare earths resulted in a larger specific surface area and a stronger absorption of visible light. At the same time, the formation of Bi2O2CO3/β-Bi2O3 heterogeneous junction enhanced the separation efficiency of photogenerated electrons and holes.
Energy Basics of Catalytic Hydrodesulfurization of Diesel Fuels
Authors: Petrova Daria , Lyubimenko Valentina , Ivanov Evgenii , Gushchin Pavel , Kolesnikov Ivan
Published: October 2022

Currently, Euro 5 (no more than 10 ppm sulfur content) and Euro 6 (less than 10 ppm sulfur content) diesel motor fuels are produced worldwide. High-quality diesel fuels are produced by removing sulfur compounds using a hydrodesulfurization process. This article is devoted to the study of hydrodesulfurization of diesel fuel containing 120 ppm of sulfur compounds in the presence of an Al-Ni-Mo-O catalyst with a 98% diesel fuel purification rate. According to the Langmuir–Hinshelwood–Panchenkov theory, a kinetic model of the process is developed with the calculation of the theoretical change in the entropy and enthalpy of the activation of the hydrodesulfurization process. The mathematical model, for the first time, takes into account the influence of the pressure of substances involved in the process on the transformation of sulfur-containing compounds. A mechanism for diesel fuel hydrotreating from sulfur-containing compounds is formulated using a generalized quantum-chemical principle. The hydrodesulfurization mechanism includes nine stages. The formulated mechanism and developed mathematical model of hydrotreating fully describe the reaction of the hydrodesulfurization of diesel fuel and show the possibility of regulating and controlling this industrially important process.
Photochemical Water Splitting via Transition Metal Oxides
Authors: Mikaeili Fateh , Gilmore Tessa , Gouma Pelagia-Iren
Published: October 2022

Rapid population growth and ever-increasing energy consumption have resulted in increased environmental pollution and energy demands in recent years. Accordingly, studies and research on innovative and efficient ways of wastewater clean-up and exploiting eco-friendly and renewable energy sources such as sunlight have become a necessity. This review focuses on recent progress with photocatalysis for water splitting capabilities. It introduces photocatalysis and hydrogen as a fuel source, before moving on to explain water splitting. Then, the criteria for ideal photocatalytic materials are discussed along with current material systems and their limitations. Finally, it concludes on the TiO2 systems and their potential in future photocatalysis research.
Novel Self-Forming Nanosized DDS Particles for BNCT: Utilizing A Hydrophobic Boron Cluster and Its Molecular Glue Effect
Authors: Fithroni Abdul Basith , Kobayashi Kazuko , Uji Hirotaka , Ishimoto Manabu , Akehi Masaru , Ohtsuki Takashi , Matsuura Eiji
Published: October 2022

BNCT is a non-invasive cancer therapy that allows for cancer cell death without harming adjacent cells. However, the application is limited, owing to the challenges of working with clinically approved boron (B) compounds and drug delivery systems (DDS). To address the issues, we developed self-forming nanoparticles consisting of a biodegradable polymer, namely, “AB-type Lactosome (AB-Lac)” loaded with B compounds. Three carborane isomers (o-, m-, and p-carborane) and three related alkylated derivatives, i.e., 1,2-dimethy-o-carborane (diC1-Carb), 1,2-dihexyl-o-carborane (diC6-Carb), and 1,2-didodecyl-o-carborane (diC12-Carb), were separately loaded. diC6-Carb was highly loaded with AB-Lac particles, and their stability indicated the “molecular glue” effect. The efficiency of in vitro B uptake of diC6-Carb for BNCT was confirmed at non-cytotoxic concentration in several cancer cell lines. In vivo/ex vivo biodistribution studies indicated that the AB-Lac particles were remarkably accumulated within 72 h post-injection in the tumor lesions of mice bearing syngeneic breast cancer (4T1) cells, but the maximum accumulation was reached at 12 h. In ex vivo B biodistribution, the ratios of tumor/normal tissue (T/N) and tumor/blood (T/Bl) of the diC6-Carb-loaded particles remained stably high up to 72 h. Therefore, we propose the diC6-Carb-loaded AB-Lac particles as a promising candidate medicine for BNCT.
Pan-Cancer Analysis of the Prognostic and Immunotherapeutic Value of MITD1
Authors: Dong Shiqiang , Hou Dingkun , Peng Yun , Chen Xiaoxu , Li Hongzheng , Wang Haitao
Published: October 2022

Microtubule-interacting and trafficking domain containing 1 (MITD1) is associated with abscission during cytokinesis. However, systematic investigation into its role in cancer is lacking. Therefore, we explored the pan-cancer role of MITD1 using multiple databases. Expression and clinical survival, immunological, and enrichment analyses were performed using R packages and online tools. For breast cancer, single-cell level analysis, immunochemistry, and in vitro experiments were performed to explore the mechanism of MITD1. A nomogram was established to predict the prognosis of patients with breast cancer and evaluate the immunotherapy biomarker based on two datasets. In some cancers, high MITD1 expression was associated with a more favorable prognosis. For instance, it inhibited tumor cell proliferation and migration in breast cancer. MITD1 may regulate cancer development by altering the tumor microenvironment, and MITD1 expression may predict the response to immune checkpoint blockade, platinum, and poly ADP-ribose polymerase inhibitor therapies. Our nomogram was used to determine the prognosis of patients with breast cancer. MITD1 can also predict the response to immunotherapy. Our first pan-cancer study of MITD1 has shown that it plays different roles in cancer development and therapy. In breast cancer, MITD1 inhibited cell proliferation and migration and serves as a new biomarker.
Recent Advancements in Molecular Therapeutics for Corneal Scar Treatment
Authors: Ghosh Anwesha , Singh Vijay K. , Singh Vivek , Basu Sayan , Pati Falguni
Published: October 2022

The process of corneal wound healing is complex and induces scar formation. Corneal scarring is a leading cause of blindness worldwide. The fibrotic healing of a major ocular wound disrupts the highly organized fibrillar collagen arrangement of the corneal stroma, rendering it opaque. The process of regaining this organized extracellular matrix (ECM) arrangement of the stromal layer to restore corneal transparency is complicated. The surface retention capacity of ocular drugs is poor, and there is a large gap between suitable corneal donors and clinical requirements. Therefore, a more efficient way of treating corneal scarring is needed. The eight major classes of interventions targeted as therapeutic tools for healing scarred corneas include those based on exosomes, targeted gene therapy, microRNAs, recombinant viral vectors, histone deacetylase inhibitors, bioactive molecules, growth factors, and nanotechnology. This review highlights the recent advancements in molecular therapeutics to restore a cornea without scarring. It also provides a scope to overcome the limitations of present studies and perform robust clinical research using these strategies.