bluuebunny/crossref_metadata_2025 huggingface dataset:
Erosive pustulöse Dermatose der Kopfhaut: häufig übersehen und selten diagnostiziert
Authors: Braun-Falco Markus
Published: February 2020

Erosive pustular dermatosis of the scalp (EPDS) is an uncommon disease and primarily affects older men who have photodamaged bald scalp, as was confirmed by our case series. EPDS is probably an overlooked disease, whose diagnosis is often missed because of a higher incidence of other cutaneous diseases affecting the same area and usually secondary to chronic actinic damage, such as actinic keratosis, basal cell carcinoma, and squamous cell carcinoma. For the first time, we report a case series of misdiagnosed EPDS with the aim of understanding why a diagnosis of EPDS was initially missed and try to give some tips to avoid future diagnostic delay.
Prospective Cohort Study to Assess the Effect of Storage Duration, Leuko-Filtration, and Gamma Irradiation on Cell-Free DNA in Red Cell Components
Authors: Gupta Nitesh , Khetan Dheeraj , Chaudhary Rajendra , Shukla Jai Shankar
Published: February 2020

Introduction: Damage to a cell and the loss of integrity of its cell membrane leads to the release of endogenous immunogenic molecules, which together are classified as “damage-associated molecular patterns” (DAMPs). Cell-free DNA (cf-DNA) released from nucleosomes may serve as a proco­agulant cofactor and may be an important mediator of immunomodulatory and proinflammatory effects associated with blood transfusion. Objectives: To assess the levels of cf-DNA in supernatants of stored red cell components and the effect of leukoreduction and gamma irradiation on the release of cf-DNA during storage. Methods: This is a prospective cohort study on 99 stored red cell components, randomly divided into three groups – buffy coat (BC)-depleted, leuko-filtered (LP), and irradiated (IR) packed red blood cells. Red cell supernatants were drawn over a period of 21 days at three different time points (day 0, 7, and 21) from the study units. cf-DNA extraction was done and quantified by a bench top fluorometer. Change in cf-DNA content, rate of change (μg/day), and percent change were estimated and compared across different groups. Results: cf-DNA content increased (p = 0.000) with storage duration in the BC (median = 238.66 μg, interquartile range [IQR] = 168.42 on day 21 vs. median = 9.44 μg, IQR = 5.23 on day 0) and IR groups (p = 0.000) (median = 245.55 μg, IQR = 253.88 on day 21 vs. median = 7.07 μg, IQR = 13.58 on day 0), while there was a decreasing trend (p = 0.032) in the LP group (median = 4.55 μg, IQR = 10.73 on day 21 vs. median = 8.66 μg, IQR = 6.56 on day 0). The median rate of change in cf-DNA content (11.13 μg/day) and percent change in cf-DNA content (median = 4,106.16%) was highest in the IR group. Conclusions: Stored red cell components contain significant amount of cf-DNA. Release of cf-DNA is further aggravated by irradiation while leukoreduction leads to a decrease in cf-DNA content.
Frequent Premature Atrial Contractions in Cryptogenic Stroke Predict Atrial Fibrillation Detection with Insertable Cardiac Monitoring
Authors: Todo Kenichi , Iwata Tomonori , Doijiri Ryosuke , Yamagami Hiroshi , Morimoto Masafumi , Hashimoto Tetsuya , Sonoda Kazutaka , Yamazaki Hidekazu , Junpei Koge , Okazaki Shuhei , Sasaki Tsutomu , Mochizuki Hideki
Published: February 2020

Objective: To determine whether frequent premature atrial contractions (PAC) predict atrial fibrillation (AF) in cryptogenic stroke patients, we analyzed the association between frequent PACs in 24-h Holter electrocardiogram recording and AF detected by insertable cardiac monitoring (ICM). Methods: We retrospectively analyzed a database of 66 consecutive patients with cryptogenic stroke who received ICM implantation between October 2016 and March 2018 at 5 stroke centers. We included the follow-up data until June 2018 in this analysis. We defined frequent PACs as the upper quartile of the 66 patients. We analyzed the association of frequent PACs with AF detected by ICM. Results: Frequent PACs were defined as >222 PACs per a 24-h period. The proportion of patients with newly detected AF by ICM was higher in patients with frequent PACs than those without (50% [8/16] vs. 22% [11/50], p < 0.05). Frequent PACs were associated with AF detection and time to the first AF after adjustment for CHADS2 score after index stroke, high plasma ­B-type natriuretic peptide (BNP; >100 pg/mL) or serum ­N-terminal pro-BNP levels (>300 pg/mL), and large left atrial diameter (≥45 mm). Conclusion: High frequency of PACs in cryptogenic stroke may be a strong predictor of AF detected by ICM.
Energetics of a Rotating Wind-forced Horizontal Convection Model of a Reentrant Channel
Authors: Zemskova Varvara E. , White Brian L. , Scotti Alberto
Published: February 2020

We present numerical results for an idealized rotating, buoyancy- and windforced channel as a simple model for the Southern Ocean branch of the Meridional Overturning Circulation (MOC). Differential buoyancy forcing is applied along the top horizontal surface, with surface cooling at one end (to represent the pole) and surface warming at the other (to represent the equatorial region) and a zonally re-entrant channel to represent the Antarctic Circumpolar Current (ACC). Zonally-uniform surface wind forcing is applied with a similar pattern to the westerlies and easterlies with varying magnitude relative to the buoyancy forcing. The problem is solved numerically using a 3D DNS model based on a finite-volume solver for the Boussinesq Navier-Stokes equations with rotation. The overall dynamics, including large-scale overturning, baroclinic eddying, turbulent mixing, and resulting energy cascades are studied by calculating terms in the energy budget using the local Available Potential Energy framework. The basic physics of the overturning in the Southern Ocean are investigated at multiple scales and the output from the fully-resolved DNS simulations is compared with the results from previous studies of the global (ECCO2) and Southern Ocean eddy-permitting state estimates. We find that both the magnitude and shape of the zonal wind stress profile are important to the spatial pattern of the overturning circulation. However, the available potential energy budget and the diapycnal mixing are not significantly affected by the surface wind stress and are primarily set by the buoyancy forcing at the surface.
Using Finger Puppets in the Primary Care Setting to Support Caregivers Talking With Their Infants: A Feasibility Pilot Study
Authors: Domek Gretchen J. , Szafran Lauren Heller , Bonnell Levi N. , Berman Stephen , Camp Bonnie W.
Published: February 2020

Disadvantaged children often show disparities in early language development. We tested the feasibility, acceptability, and preliminary efficacy of introducing finger puppets in the primary care setting at the 2-month well visit to support caregivers talking with their infants. Caregivers completed a sociodemographic survey and were contacted by phone 2 weeks later to assess initial usage and satisfaction. Ages & Stages Questionnaires (ASQ-3) were independently recorded at well visits. A family cumulative risk score was calculated from the sociodemographic survey. Thirty-four caregiver-child pairs were enrolled. Caregivers reported high satisfaction with the intervention. ASQ-3 Communication and Total scores at 6, 12, and 18 months were significantly higher for high puppet users across all age levels with no significant interactions with age or cumulative risk. Finger puppets provide a low-cost way to promote language-rich interactions. Preliminary evidence suggests that high puppet usage may have long-lasting effects on child development and should be further evaluated.
Spatially organizing future genders: An artistic intervention in the creation of a hir-toilet
Authors: Skoglund Annika , Holt Robin
Published: February 2020

Toilets, a neglected facility in the study of human relations at work and beyond, have become increasingly important in discussions about future experiences of gender diversity. To further investigate the spatial production of gender and its potential expressions, we transformed a unisex single-occupancy toilet at Uppsala University into an all-gender or ‘hir-toilet’.1 With the aim to disrupt and expose the dominant spatial organization of the two binary genders, we inaugurated the hir-toilet with the help of a performance artist. We describe and analyse internal and external responses thereto, using Lefebvre’s work on dialectics and space. Focusing on how space is variously lived, conceived and perceived, our analysis questions the very rationale of gender categorizations. The results contribute to a renewed critique of binary thinking in the organization of workplaces by extending our understanding of how space and human relations mutually constitute each other.
Indonesian online newspaper reporting of suicidal behavior: Compliance with World Health Organization media guidelines
Authors: Nisa Nazriatun , Arifin Muhammad , Nur Muhammad Fauzan , Adella Shylvana , Marthoenis Marthoenis
Published: February 2020

Background:

Despite the lack of attention, suicide is a major public and mental health problem in Indonesia. Research on how Indonesian newspapers portray suicide case has been lacking.

Aim:

To assess the quality of Indonesian online newspaper reporting suicide news and its compliance with the World Health Organization (WHO) mass media reporting guidelines.

Methods:

The content of suicide news reported in online newspapers was searched and scrutinized against the WHO suicide reporting guidelines.

Results:

Within the duration of 6 months, there were 548 suicide news reported. This consisted of 16.8% attempted suicide and 83.2% completed suicide. Approximately 90.3% reported age, 97.3% reported gender and 64.3% reported the marital status of the victims. Furthermore, 68% reported the methods of suicide in headline or title, 40.5% shown the picture of the victims and 56.6% shown illustration only, and 67.2% reported life event related to suicide act.

Conclusion:

The vast majority of online newspapers in Indonesia exaggerate suicide news. They rarely inform the readers that there is hope and help available. The majority also do not conform to the WHO media guidelines.
Sexually transmitted infection diagnoses, sexualised drug use and associations with pre-exposure prophylaxis use among men who have sex with men in the UK
Authors: Hibbert Matthew P , Brett Caroline E , Porcellato Lorna A , Hope Vivian D
Published: February 2020

Previous research has focused on acceptability of pre-exposure prophylaxis (PrEP) use, but few community-based studies have been conducted regarding actual use, and PrEP use in the context of sexualised drug use remains understudied. A national online cross-sectional study recruited men who have sex with men (MSM) via social media (April–June 2018). Multivariable logistic regression was used to investigate factors associated with PrEP use. Bivariate analyses compared engaging in condomless anal intercourse (CAI) under the influence of specific drugs and recent sexually transmitted infection (STI) diagnoses (past 12 months) between MSM taking PrEP and those not. Overall, 6% (99/1581) MSM reported current PrEP use. Factors associated with PrEP use were increasing age, recent genitourinary medicine (GUM) attendance (95% versus 45%, aOR = 6.25, 95%CI 2.05, 19.03), an HIV test in the past three months (89% versus 23%, aOR = 14.22, 95%CI 6.76, 29.90), and recent engagement in chemsex (21% versus 4%, aOR = 3.56, 95%CI 1.78, 7.11). MSM taking PrEP were more likely to have had an STI diagnosis (42% versus 8%), most commonly chlamydia (26% versus 3%) and gonorrhoea (25% versus 4%). Considering the elevated levels of self-reported STI diagnoses among those on PrEP, there was a high level of engagement with sexual health services, which may help reduce onward STI transmission.
Implementing Mycoplasma genitalium testing across a London-based sexual health service: A quality improvement project
Authors: Khan H , Alam V , Yin L , Tincknell L , Wallis E , Sethi G
Published: February 2020

Recent national guidelines recommended testing for Mycoplasma genitalium (MG) in clinically-indicated conditions (CIC) including non-gonococcal urethritis (NGU), pelvic inflammatory disease (PID) and epididymo-orchitis. Over five months in 2018 a quality improvement project (QIP) was carried out across three London sexual health clinics with the aim of increasing MG testing rates in CICs. Three Plan-Do-Study-Act (PDSA) cycles were completed: improving IT access, an education event and reminder emails for clinicians who did not test in CIC. To measure testing rates ten patients from each CIC were randomly selected each week and MG testing outcomes were collected. As a balancing measure, we identified the rate of inappropriate MG testing. MG testing rates in patients with NGU increased to 90% following QIP initiation (baseline rate 60%) and this increase was sustained. No increase in MG testing was seen in PID and epididymo-orchitis. Inappropriate MG test rates were high (median of 11%) but remained constant throughout the QIP period. As MG testing is expanding across the UK, we outline a QIP integrating MG testing into a busy multi-site, sexual health service improving testing uptake while not increasing inappropriate testing.
Torpedo maculopathy: A case series – insights into basic pathology
Authors: Kerwat Doaa , Jamall Omer , Antonakis Serafeim , Almeida Goncalo C
Published: February 2020

Torpedo maculopathy is a benign retinal disorder whose pathophysiological origins are currently poorly understood. A number of theories have been postulated, with stable developmental anomalies of the retinal pigment epithelium taking the forefront. Four clinical cases are outlined of patients with macular torpedo lesions, with differing clinical presentation. In all four cases, this reveals very thin retinal pigment epithelium and outer retina associated with the lesion. In a single case, the oldest patient of the group, there is the additional finding of subretinal and intraretinal fluid accumulation. The contrast between this case and the other cases suggests that while initially this benign pathology might start with structurally normal retina with no fluid accumulation, dysgenetic changes in the retinal pigment epithelium might lead to secondary accumulation of fluid over time. Whether indeed this disorder might be progressive in nature, or whether in fact it is a static, non-progressive developmental abnormality as formerly thought, requires further elucidation.
Rutin promotes osteogenic differentiation of periodontal ligament stem cells through the GPR30-mediated PI3K/AKT/mTOR signaling pathway
Authors: Zhao Bin , Xiong Yixuan , Zhang Yunpeng , Jia Linglu , Zhang Wenjing , Xu Xin
Published: February 2020

Rutin is one of the flavonoids found in fruits and vegetables. Recent reports have revealed that rutin is a major player in proliferation and bone development. However, data on how rutin regulates the proliferation of periodontal ligament stem cells (PDLSCs), as well as the differentiation of osteogenic cells are scanty. Here, our findings showed that rutin enhanced PDLSCs proliferation, increased ALP activity, and matrix mineralization. Moreover, rutin significantly promoted the expression of osteogenic genes and elevated phosphorylated AKT and mTOR. Treatment with LY294002 reversed these effects by inhibiting PI3K. We also found that the expression levels of GPR30 were increased by rutin. Interestingly, this upregulation was not altered after the addition of LY294002. In addition, G15, a selective antagonist of GPR30, could reduce the beneficial effects induced by rutin and interfere with the modulation of PI3K/AKT/mTOR signal transduction. Collectively, our findings revealed that rutin increased proliferation and osteogenic differentiation of PDLSCs through GPR30-mediated PI3K/AKT/mTOR signal transduction. Therefore, it could be deduced that rutin as a certain flavonoid possesses therapeutic value for periodontal bone regeneration and tissue engineering.

Impact statement

In our study, the effects and mechanisms of rutin on the osteogenic differentiation and proliferation of PDLSCs were investigated. Our findings might provide basic knowledge and guidance to understand and use rutin in the bioengineering of the periodontal tissues and regeneration of bones. The following is a short description of the main findings: rutin promotes the osteogenic differentiation and proliferation of PDLSCs; PI3K/AKT/mTOR signal pathway mediates the effects of rutin on PDLSCs; rutin activates PI3K/AKT/mTOR signal pathway via GPR30.
Regioselective synthesis of new 7,8-dichlorobenzofuro[3,2-c]quinoline-6,9,10(5H)-triones from reactions of 4-hydroxy-2-quinolones with 3,4,5,6-tetrachloro-1,2-benzoquinone
Authors: Aly Ashraf A , Hassan Alaa A , Mohamed Nasr K , Abd El-Haleem Lamiaa E , Bräse Stefan
Published: February 2020

A novel series of 7,8-dichlorobenzofuro[3,2- c]quinoline-6,9,10(5 H)-triones was obtained regioselectively in good yields. The products were formed by the reactions of the 4-hydroxy-2(1 H)-quinolinones with 3,4,5,6-tetrachloro-1,2-benzoquinone in tetrahydrofuran as the solvent. Infrared, nuclear magnetic resonance (two-dimensional nuclear magnetic resonance), mass spectra and elemental analysis were used to elucidate the structures of new compounds.
Application of Uniform Experimental Design in Optimizing Excitation Parameters for Magnetic Frequency Mixing Measurements
Authors: Chang Yu , Jiao Jingpin , Liu Xiucheng , Li Guanghai , He Cunfu , Wu Bin
Published: February 2020

Excitation parameter preferences are key factors affecting the performance of magnetic frequency mixing detection. A uniform experimental design method was used to analyze this influence. Using fuzzy theory, a comprehensive model is established for evaluating the effect of magnetic frequency mixing. A polynomial is selected as the regression function to express explicitly the correlation between the excitation parameters and the frequency-mixing effect. The excitation parameters were then optimized using genetic algorithm. Magnetic frequency mixing experiments were conducted to measure the surface hardness of some ferromagnetic materials. Frequency mixing is further enhanced under the optimal settings, resulting in an improvement in the measurement sensitivity. The results of this study support the application of the magnetic frequency mixing technique in non-destructive testing.
Cognitive correlates of attention-deficit hyperactivity disorder in children and adolescents with high intellectual ability
Authors: Cadenas María , Hartman Catharina , Faraone Stephen , Antshel Kevin , Borges África , Hoogeveen Lianne , Rommelse Nanda
Published: February 2020

Background

There is an ongoing debate as to whether attention-deficit hyperactivity disorder (ADHD) in highly intelligent individuals has a similar presentation as in average intelligent individuals. The aim of this study was to examine the cognitive correlates of ADHD in highly intelligent children and adolescents with ADHD.

Method

Two independent samples (N = 204 and N = 84) of (1) high intelligence quotient (IQ) (IQ ≥ 120) children and adolescents with ADHD were used, carefully matched on age, gender, ADHD severity, and IQ with (2) control participants with high intelligence, (3) participants with ADHD with an average intelligence (IQ 90–110), and (4) control participants with an average intelligence. These samples were selected from the Dutch node of the International Multicenter ADHD Genetics (NeuroIMAGE) and Tracking Adolescents’ Individual Lives Survey (TRAILS) cohorts, respectively, in which a large battery of cognitive tasks was administered. Linear mixed models were used to examine the main effects of ADHD and IQ and their interaction on cognitive performance.

Results

ADHD-control group differences were not moderated by IQ; mostly equally large ADHD-control differences in cognitive performance were found for high versus average intelligent groups. The small moderating effects found mostly indicated somewhat milder cognitive problems in highly intelligent individuals with ADHD. Overall, highly intelligent children and adolescents with ADHD performed at the level of the average intelligent control children.

Conclusions

Our findings indicate the cognitive profile of ADHD is similar in highly versus average intelligent individuals with ADHD, although ADHD-related cognitive deficits may be easily overlooked in the high intelligence population when compared to the typical (i.e., average intelligent) control group.
The predictive ability of indirect genetic models is reduced when culled animals are omitted from the data
Authors: Ask Birgitte , Christensen Ole F. , Heidaritabar Marzieh , Madsen Per , Nielsen Hanne M.
Published: February 2020

Background

Physical removal of individuals from groups causes reductions in group sizes and changes in group composition, which may affect the predictive ability of estimates of indirect genetic effects of animals on phenotypes of group mates. We hypothesized that including indirect genetic effects of culled animals and of animals without phenotypes in the analysis affects estimates of genetic parameters, improves predictive ability, and reduces bias of predicted breeding values. We tested this by applying different editing procedures, i.e. omission of individuals or groups from the data, and genetic models, i.e. a classical and an indirect genetic model (IGM) without or with weighting of indirect genetic effects based on the relative proportion of time spent in the pen or space allowance. Data consisted of average daily gain for 123,567 pigs in 11,111 groups, from which 3% of individuals in 25% of groups were prematurely removed from the group.

Results

The estimate of total heritability was higher (0.29 to 0.34) than that of direct heritability (0.23 to 0.25) regardless of the editing procedures and IGM used. Omission of individuals or groups from the data reduced the predictive ability of estimates of indirect genetic effects by 8 to 46%, and the predictive ability of estimates of the combined direct and indirect genetic effects by up to 4%. Omission of full groups introduced bias in predicted breeding values. Weighting of indirect genetic effects reduced the predictive ability of their estimates by at least 19% and of the estimates of the combined direct and indirect genetic effects by 1%.

Conclusions

We identified significant indirect genetic effects for growth in pigs. Culled animals should neither be removed from the data nor accounted for by weighting their indirect genetic effects in the model based on the relative proportion of time spent in the pen or space allowance, because it will reduce predictive ability and increase bias of predicted breeding values. Information on culled animals is important for prediction of indirect genetic effects and must be accounted for in IGM analyses by including fixed regressions based on relative time spent within the pen or relative space allowance.
Correction to: The performance of coalescent-based species tree estimation methods under models of missing data
Authors: Nute Michael , Chou Jed , Molloy Erin K. , Warnow Tandy
Published: February 2020

After publication of [1], the authors were informed by John A. Rhodes of a counterexample to Theorem 11 of [1].
Antibiotic use among twelve Canadian First Nations communities: a retrospective chart review of skin and soft tissue infections
Authors: Jeong Dahn , Nguyen Ha Nhan Thi , Tyndall Mark , Schreiber Yoko S.
Published: February 2020

Background

Previous publications indicated an emerging issue with community-acquired methicillin-resistantStaphylococcus aureus(CA-MRSA), particularly skin and soft tissue infections (SSTIs), in Indigenous communities in Canada. The objectives of this analysis were to explore the prevalence of SSTIs due to CA-MRSA and patterns of antimicrobial use in the community setting.

Methods

A retrospective chart review was conducted as part of an environmental scan to assess antibiotic prescriptions in 12 First Nations communities across five provinces in Canada including Alberta, Saskatchewan, Manitoba, Ontario, and Québec. Charts were randomly selected from nursing stations and patients who had accessed care in the previous 12 months and were ≥ 18 years were included in the review. Data was collected from September to December, 2013 on antibiotic prescriptions, including SSTIs, clinical symptoms, diagnostic information including presence of CA-MRSA infection, and treatment.

Results

A total of 372 charts were reviewed, 60 from Alberta, 70 from Saskatchewan, 120 from Manitoba, 100 from Ontario, and 22 from Québec. Among 372 patients, 224 (60.2%) patients had at least one antibiotic prescription in the previous 12 months and 569 prescriptions were written in total. The prevalence of SSTIs was estimated at 36.8% (137 cases of SSTIs in 372 charts reviewed). In 137 cases of SSTIs, 34 (24.8%) were purulent infections, and 55 (40.2%) were due to CA-MRSA.

Conclusions

This study has identified a high prevalence of antibiotic use and SSTIs due to CA-MRSA in remote and isolated Indigenous communities across Canada. This population is currently hard to reach and under-represented in standard surveillance system and randomized retrospective chart reviews can offer complimentary methodology for monitoring disease burden, treatment and prevention.
Thinking in paracoccidioidomycosis: a delayed diagnosis of a neglected tropical disease, case report and review of clinical reports and eco-epidemiologic data from Colombia since the 2000
Authors: Arias Ramos Deving , Alzate John Alexander , Giraldo Montoya Ángela María , Trujillo Yessica Andrea , Arias Ramos Leidy Yurany
Published: February 2020

Background

Paracoccidioidomycosis is a neglected tropical disease, endemic in several countries of South America including Colombia. We report a case of a patient with Chronic Multifocal Paracoccidioidomycosis with long-standing symptoms and a delayed diagnosis caused by several barriers to achieve it. We did a review of the papers published in Colombia about this disease, focusing in clinical data and eco-epidemiology with the finding of a lack of new information on this topic since the 2000 in our region.

Case presentation

We present a 54-year-old man, farmer in his youth, with a chronic ulcerated lesion in the lower lip similar to a lip carcinoma, a deforming lesion in the nose, and respiratory symptoms with emphysematous lung. Lip biopsy with silver methenamine stain revealed small and large budding yeasts that resembles a “mariner’s wheel” confirming Chronic Multifocal Paracoccidioidomycosis. He was treated successfully but subsequently lost to follow up.

Conclusions

It is very important to focus attention, reinforce the search and create networks for the study of neglected tropical diseases. The presented case illustrates a usual clinical presentation, but with a delayed diagnosis due to the difficulties that still occur in some regions like ours for the early recognition of a case of chronic multifocal paracoccidioidomycosis.
Sacroiliac joint variation associated with diffuse idiopathic skeletal hyperostosis
Authors: Yahara Yasuhito , Yasuda Taketoshi , Kawaguchi Yoshiharu , Suzuki Kayo , Seki Shoji , Kondo Miho , Makino Hiroto , Kamei Katsuhiko , Kanamori Masahiko , Kimura Tomoatsu
Published: February 2020

Background

Diffuse idiopathic skeletal hyperostosis (DISH) is characterized by the ossification of vertebral bodies and peripheral entheses. However, variations in sacroiliac (SI) joint change in patients with DISH have not been fully clarified. The purpose of this study was to evaluate SI joint variation in patients with DISH in comparison with a non-DISH population.

Methods

A total of 342 SI joints in 171 patients (DISH+, n = 86; DISH-, n = 85) who had undergone lumbar spine surgery were analyzed by computed tomography examination. SI joint variations were classified into four types: Type 1, normal or tiny peripheral bone irregularity; Type 2, subchondral bone sclerosis and osteophytes formation; Type 3, vacuum phenomenon; and Type 4, bridging osteophyte and bony fusion. The type of bridging osteophyte in SI joints and the prevalence of ossification in each spinal segment from C1 to SI joint were also examined.

Results

The most common SI joint variation in the DISH+ group was bony fusion (Type 4), with 71.6% exhibiting anterior paraarticular bridging. On the other hand, SI joint vacuum phenomenon (Type 3) was the most frequent change (57.1%) in the DISH- group. The middle to lower thoracic spine and SI joints were highly affected in DISH and caused bony ankylosis.

Conclusions

Anterior paraarticular bridging was the most common type of SI joint change in patients with DISH who underwent lumbar spine surgery. The present results regarding variations of SI joint changes in DISH should help understand the etiology of DISH.
Barriers and opportunities for breast cancer organizations to focus on environmental health and disease prevention: a mixed-methods approach using website analyses, interviews, and focus groups
Authors: Ohayon Jennifer Liss , Nost Eric , Silk Kami , Rakoff Michele , Brody Julia Green
Published: February 2020

Background

Breast cancer is the most commonly diagnosed cancer among women worldwide and most cases are not due to high risk inherited genes. In response, breast cancer activists successfully advocated for innovative research on environmental chemical exposures as a possible cause. Since then, new evidence supports hypotheses that common industrial and consumer chemicals are linked to the disease, and expert panels recommend reducing exposures. We evaluated whether these research results and recommendations are translated back into the work of breast cancer organizations and what barriers and opportunities influence their ability to focus on environmental factors.

Methods

We used a Python script to evaluate the frequency of environmental terms on the websites of 81 breast cancer organizations (> 14,000 associated URLs) and conducted two focus groups and 20 interviews with leaders of breast cancer organizations. We also analyzed the frequency of terms on two trusted, national cancer websites.

Results

40% of organizations include information on environmental chemicals on their websites, but references are infrequent and rarely cite specific chemicals of concern. Most organizations (82%) discuss other risk factors such as exercise, diet, family history, or genetics. From interviews and focus groups, we identified four types of barriers to addressing environmental chemicals: 1) time and resource constraints, 2) limited knowledge of the state of the research and lack of access to experts, 3) difficulties with messaging, including concern that cultural and economic factors make it difficult for individuals to reduce their exposures, and 4) institutional obstacles, such as the downplaying of environmental risks by industry interests. Participants expressed the desire for easy-to-adopt educational programs and increased federal funding for scientist-advocate research partnerships.

Conclusion

Our research underscores the need for environmental breast cancer experts and trusted cancer organizations to increase research translation activities so that breast cancer organizations can communicate new science on environmental factors in their online and in-person work. Moreover, our research highlights how most groups are focusing on providing resources to diagnosed women, including addressing problems with healthcare access, which displaces their ability to work on breast cancer prevention.
MRI radiomics for early prediction of response to vaccine therapy in a transgenic mouse model of pancreatic ductal adenocarcinoma
Authors: Eresen Aydin , Yang Jia , Shangguan Junjie , Li Yu , Hu Su , Sun Chong , Velichko Yury , Yaghmai Vahid , Benson Al B. , Zhang Zhuoli
Published: February 2020

Background

There is a lack of well-established clinical tools for predicting dendritic cell (DC) vaccination response of pancreatic ductal adenocarcinoma (PDAC). DC vaccine treatment efficiency was demonstrated using histological analysis in pre-clinical studies; however, its usage was limited due to invasiveness. In this study, we aimed to investigate the potential of MRI texture features for detection of early immunotherapeutic response as well as overall survival (OS) of PDAC subjects following dendritic cell (DC) vaccine treatment in LSL-KrasG12D;LSL-Trp53R172H;Pdx-1-Cre (KPC) transgenic mouse model of pancreatic ductal adenocarcinoma (PDAC).

Materials and methods

KPC mice were treated with DC vaccines, and tumor growth was dynamically monitored. A total of a hundred and fifty-two image features of T2-weighted MRI images were analyzed using a kernel-based support vector machine model to detect treatment effects following the first and third weeks of the treatment. Moreover, univariate analysis was performed to describe the association between MRI texture and survival of KPC mice as well as histological tumor biomarkers.

Results

OS for mice in the treatment group was 54.8 ± 22.54 days while the control group had 35.39 ± 17.17 days. A subset of three MRI features distinguished treatment effects starting from the first week with increasing accuracy throughout the treatment (75% to 94%). Besides, we observed that short-run emphasis of approximate wavelet coefficients had a positive correlation with the survival of the KPC mice (r = 0.78, p < 0.001). Additionally, tissue-specific MRI texture features showed positive association with fibrosis percentage (r = 0.84, p < 0.002), CK19 positive percentage (r = − 0.97, p < 0.001), and Ki67 positive cells (r = 0.81, p < 0.02) as histological disease biomarkers.

Conclusion

Our results demonstrate that MRI texture features can be used as imaging biomarkers for early detection of therapeutic response following DC vaccination in the KPC mouse model of PDAC. Besides, MRI texture can be utilized to characterize tumor microenvironment reflected with histology analysis.
CLEAR: coverage-based limiting-cell experiment analysis for RNA-seq
Authors: Walker Logan A. , Sovic Michael G. , Chiang Chi-Ling , Hu Eileen , Denninger Jiyeon K. , Chen Xi , Kirby Elizabeth D. , Byrd John C. , Muthusamy Natarajan , Bundschuh Ralf , Yan Pearlly
Published: February 2020

Background

Direct cDNA preamplification protocols developed for single-cell RNA-seq have enabled transcriptome profiling of precious clinical samples and rare cell populations without the need for sample pooling or RNA extraction. We term the use of single-cell chemistries for sequencing low numbers of cells limiting-cell RNA-seq (lcRNA-seq). Currently, there is no customized algorithm to select robust/low-noise transcripts from lcRNA-seq data for between-group comparisons.

Methods

Herein, we present CLEAR, a workflow that identifies reliably quantifiable transcripts in lcRNA-seq data for differentially expressed genes (DEG) analysis. Total RNA obtained from primary chronic lymphocytic leukemia (CLL) CD5+ and CD5− cells were used to develop the CLEAR algorithm. Once established, the performance of CLEAR was evaluated with FACS-sorted cells enriched from mouse Dentate Gyrus (DG).

Results

When using CLEAR transcripts vs. using all transcripts in CLL samples, downstream analyses revealed a higher proportion of shared transcripts across three input amounts and improved principal component analysis (PCA) separation of the two cell types. In mouse DG samples, CLEAR identifies noisy transcripts and their removal improves PCA separation of the anticipated cell populations. In addition, CLEAR was applied to two publicly-available datasets to demonstrate its utility in lcRNA-seq data from other institutions. If imputation is applied to limit the effect of missing data points, CLEAR can also be used in large clinical trials and in single cell studies.

Conclusions

lcRNA-seq coupled with CLEAR is widely used in our institution for profiling immune cells (circulating or tissue-infiltrating) for its transcript preservation characteristics. CLEAR fills an important niche in pre-processing lcRNA-seq data to facilitate transcriptome profiling and DEG analysis. We demonstrate the utility of CLEAR in analyzing rare cell populations in clinical samples and in murine neural DG region without sample pooling.
Intraoperative surgical site infection control and prevention: a position paper and future addendum to WSES intra-abdominal infections guidelines
Authors: De Simone Belinda , Sartelli Massimo , Coccolini Federico , Ball Chad G. , Brambillasca Pietro , Chiarugi Massimo , Campanile Fabio Cesare , Nita Gabriela , Corbella Davide , Leppaniemi Ari , Boschini Elena , Moore Ernest E. , Biffl Walter , Peitzmann Andrew , Kluger Yoram , Sugrue Michael , Fraga Gustavo , Di Saverio Salomone , Weber Dieter , Sakakushev Boris , Chiara Osvaldo , Abu-Zidan Fikri M. , ten Broek Richard , Kirkpatrick Andrew W. , Wani Imtiaz , Coimbra Raul , Baiocchi Gian Luca , Kelly Micheal D. , Ansaloni Luca , Catena Fausto
Published: February 2020

Background

Surgical site infections (SSI) represent a considerable burden for healthcare systems. They are largely preventable and multiple interventions have been proposed over past years in an attempt to prevent SSI.

We aim to provide a position paper on Operative Room (OR) prevention of SSI in patients presenting with intra-abdominal infection to be considered a future addendum to the well-known World Society of Emergency Surgery (WSES) Guidelines on the management of intra-abdominal infections.

Methods

The literature was searched for focused publications on SSI until March 2019. Critical analysis and grading of the literature has been performed by a working group of experts; the literature review and the statements were evaluated by a Steering Committee of the WSES.

Results

Wound protectors and antibacterial sutures seem to have effective roles to prevent SSI in intra-abdominal infections. The application of negative-pressure wound therapy in preventing SSI can be useful in reducing postoperative wound complications.

It is important to pursue normothermia with the available resources in the intraoperative period to decrease SSI rate.

The optimal knowledge of the pharmacokinetic/pharmacodynamic characteristics of antibiotics helps to decide when additional intraoperative antibiotic doses should be administered in patients with intra-abdominal infections undergoing emergency surgery to prevent SSI.

Conclusions

The current position paper offers an extensive overview of the available evidence regarding surgical site infection control and prevention in patients having intra-abdominal infections.
Y-box binding protein-1 is crucial in acquired drug resistance development in metastatic clear-cell renal cell carcinoma
Authors: D’Costa Ninadh M. , Lowerison Matthew R. , Raven Peter A. , Tan Zheng , Roberts Morgan E. , Shrestha Raunak , Urban Matthew W. , Monjaras-Avila Cesar U. , Oo Htoo Zarni , Hurtado-Coll Antonio , Chavez-Munoz Claudia , So Alan I.
Published: February 2020

Background

Renal cell carcinoma (RCC) is a highly vascular tumor and patients with low risk metastatic RCC of clear-cell histological sub-type (mccRCC) are treated with tyrosine-kinase inhibitors (TKIs), sunitinib, as the first-line of treatment. Unfortunately, TKI resistance eventually develops, and the underlying molecular mechanism is not well understood.

Methods

RCC cell-line with metastatic clear-cell histology (Caki-1), and patient samples were analysed to identify the role of Y-box binding protein 1 (YB-1) and ATP-binding cassette sub-family B member 1 (ABCB-1) in acquired sunitinib-resistance development. Caki-1 was conditioned with increasing sunitinib doses to recapitulate acquired resistance development in clinics. Sunitinib-conditioned and wild-type Caki-1 were subjected to cell viability assay, scratch assay, chicken embryo chorioallantoic membrane engraftment and proteomics analysis. Classical biochemical assays like flow cytometry, immunofluorescent staining, immunohistochemical staining, optical coherence tomography imaging, Western Blot and RT-PCR assays were applied to determine the possible mechanism of sunitinib-resistance development and the effect of drug treatments. Publicly available data was also used to determine the role of YB-1 upregulation in ccRCC and the patients’ overall survival.

Results

We demonstrate that YB-1 and ABCB-1 are upregulated in sunitinib-resistant in vitro, ex vivo, in vivo and patient samples compared to the sensitive samples. This provides evidence to a mechanism of acquired sunitinib-resistance development in mccRCC. Furthermore, our results establish that inhibiting ABCB-1 with elacridar, in addition to sunitinib, has a positive impact on reverting sunitinib-resistance development in in vitro, ex vivo and in vivo models.

Conclusion

This work proposes a targeted therapy (elacridar and sunitinib) to re-sensitize sunitinib-resistant mccRCC and, possibly, slow disease progression.
Arenavirus as a potential etiological agent of odontogenic tumours in humans
Authors: de Feo Marco , De Leo Cristina , Romeo Umberto , Muti Paola , Blandino Giovanni , Di Agostino Silvia
Published: February 2020

Odontogenic tumors (OT) are considered rare events and their epidemiologic data are scarce and under-estimated in developing countries because there is no systematic collection of clinical features including histological analyses of the tissue samples. Furthermore, there is an underestimation of the disease relevance and affected people are often marginalized in spite of severe functional impairment of aero-digestive tract. Etiology of OT in humans is still unknown and it represents an important therapeutic and diagnostic challenge.

Lassa fever is an acute viral haemorrhagic illness caused by Lassa virus, a member of the arenavirus family of viruses. The disease is endemic in the rodent population in West-East Africa. Humans usually become infected with Lassa virus through exposure to the food or household items contaminated with urine or feces of infected rats. It is also reported person-to-person infections. About 80% of people infected by Lassa virus have no symptoms but the virus establishes a life-long persistent infection.

The present commentary significance is to start, for the first time ever, a systematic collection of clinical features and tissue sample collection at the St. Mary’s Hospital in Lacor (Gulu) North Uganda where the considered pathologies have an important frequency. The systematic collection will allow to corroborate the possible association between arenaviruses infection and pathogenesis of odontogenic tumors in humans.
Elucidating Decorin’s role in the preovulatory follicle
Authors: Kedem A. , Ulanenko-Shenkar K. , Yung Y. , Yerushalmi G. M. , Maman E. , Hourvitz A.
Published: February 2020

Background

DCN (decorin) is a proteoglycan known to be involved in regulating cell proliferation, collagen fibril organization and migration. In our global transcriptome RNA-sequencing approach to systematically identify new ovulation-associated genes, DCN was identified as one of the highly regulated genes. We therefore hypothesize that DCN may have a role in ovulatory processes such as cell migration and proliferation.

Aim

To characterize the expression, regulation and function of the proteoglycan DCN in the human ovarian follicles during the preovulatory period.

Methods

The in-vivo expression of DCN mRNA in mural (MGCs) and cumulus (CGCs) granulosa cells was characterized using quantitative RT-PCR and western blot.

A signaling study was performed by treating human MGCs cultures with gonadotropins and different stimulators and inhibitors to determine their effect on DCN expression by qRT- PCR and elucidate the pathways regulating these proteins.

In a functional study, KGN granulosa cell line was used to study cell migration with a scratch assay.

Results

DCN mRNA expression was significantly higher in MGCs compared to CGCs. DCN mRNA was significantly higher in CGCs surrounding mature metaphase II (MII) oocytes compared to CGCs of germinal vesicle (GV) and metaphase I (MI) oocytes.

hCG significantly increased DCN mRNA and protein expression levels in cultured MGCs. Using signal transduction activators and inhibitors, we demonstrated that DCN induction by LH/hCG is carried out via PKA, PKC, ERK/MEK, and PI3K pathways.

We showed that DCN expression is also induced in high-density cell cultures, in a dose-dependent pattern. In addition, progesterone induced a significant increase in DCN secretion to the media. MGCs from follicles of endometriosis patients exhibited reduced (about 20% of) mRNA transcriptions levels compared to MGCs follicles of control patients. More significantly, we found that DCN has an inhibiting effect on KGN cell migration.

Conclusions

Our study indicates that DCN is a unique ovulatory gene.

Our findings support the hypothesis that DCN plays an important new role during the preovulatory period and ovulation, and stress its involvement in endometriosis infertility. A better understanding of DCN role in ovulation and endometriosis may provide treatment for some types of infertility.
A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells
Authors: Lee Sooncheol , Micalizzi Douglas , Truesdell Samuel S. , Bukhari Syed I. A. , Boukhali Myriam , Lombardi-Story Jennifer , Kato Yasutaka , Choo Min-Kyung , Dey-Guha Ipsita , Ji Fei , Nicholson Benjamin T. , Myers David T. , Lee Dongjun , Mazzola Maria A. , Raheja Radhika , Langenbucher Adam , Haradhvala Nicholas J. , Lawrence Michael S. , Gandhi Roopali , Tiedje Christopher , Diaz-Muñoz Manuel D. , Sweetser David A. , Sadreyev Ruslan , Sykes David , Haas Wilhelm , Haber Daniel A. , Maheswaran Shyamala , Vasudevan Shobha
Published: February 2020

Background

Quiescence (G0) is a transient, cell cycle-arrested state. By entering G0, cancer cells survive unfavorable conditions such as chemotherapy and cause relapse. While G0 cells have been studied at the transcriptome level, how post-transcriptional regulation contributes to their chemoresistance remains unknown.

Results

We induce chemoresistant and G0 leukemic cells by serum starvation or chemotherapy treatment. To study post-transcriptional regulation in G0 leukemic cells, we systematically analyzed their transcriptome, translatome, and proteome. We find that our resistant G0 cells recapitulate gene expression profiles of in vivo chemoresistant leukemic and G0 models. In G0 cells, canonical translation initiation is inhibited; yet we find that inflammatory genes are highly translated, indicating alternative post-transcriptional regulation. Importantly, AU-rich elements (AREs) are significantly enriched in the upregulated G0 translatome and transcriptome. Mechanistically, we find the stress-responsive p38 MAPK-MK2 signaling pathway stabilizes ARE mRNAs by phosphorylation and inactivation of mRNA decay factor, Tristetraprolin (TTP) in G0. This permits expression of ARE mRNAs that promote chemoresistance. Conversely, inhibition of TTP phosphorylation by p38 MAPK inhibitors and non-phosphorylatable TTP mutant decreases ARE-bearing TNFα and DUSP1 mRNAs and sensitizes leukemic cells to chemotherapy. Furthermore, co-inhibiting p38 MAPK and TNFα prior to or along with chemotherapy substantially reduces chemoresistance in primary leukemic cells ex vivo and in vivo.

Conclusions

These studies uncover post-transcriptional regulation underlying chemoresistance in leukemia. Our data reveal the p38 MAPK-MK2-TTP axis as a key regulator of expression of ARE-bearing mRNAs that promote chemoresistance. By disrupting this pathway, we develop an effective combination therapy against chemosurvival.