bluuebunny/crossref_metadata_2025 huggingface dataset:
Circulating MicroRNAs as Specific Biomarkers in Atrial Fibrillation: A Meta-Analysis
Authors: Menezes Junior Antônio da Silva , Ferreira Lara Cristina , Barbosa Laura Júlia Valentim , Silva Daniela de Melo e , Saddi Vera Aparecida , Silva Antonio Márcio Teodoro Cordeiro
Published: February 2023

Atrial fibrillation (AF) is the most frequently occurring supraventricular arrhythmia. Although microRNAs (miRNAs) have been associated with AF pathogenesis, standard protocols for quantifying and selecting specific miRNAs for clinical use as biomarkers should be optimized. In this study, we evaluated the clinical application of miRNAs as biomarkers for the prognosis and diagnosis of AF. Literature searches were conducted on PubMed, Cochrane Library, and EMBASE. We included prospective or retrospective observational studies that had been published as of 14 February 2022; our main objective was to analyze the relationship between circulating miRNAs and AF. The data were extracted using the descriptors “Atrial fibrillation AND miRNA”, “Atrial fibrillation AND diagnostic AND miRNA”, and “Atrial fibrillation AND prognosis AND miRNA”. No filters were applied for period delimitation, type of publication, or language. Studies using samples isolated from blood plasma and TaqMan and RT-qPCR for detecting and quantifying miRNAs were selected, and those that used atrial tissue samples were excluded. We identified 272 articles and excluded 102 duplicated articles. Two authors independently read the titles and abstracts of 170 out of 272 articles and selected 56 potential articles, 6 of which were selected for final review. Our analysis revealed a significant association between AF and miR-4798 [OR = 1.90 (95% CI 1.45–2.47)], AF and miRNA-133a [2.77 (2.73–2.82)], AF and miRNA-150 [3.77 (1.50–9.46); I2 = 70%], AF and miRNA-21 [2.23 (1.20–4.17); I2 = 99%], AF and hsa-miRNA4443 [2.32 (2.20–2.44)], and AF and miR-20a-5p [3.67 (1.42–9.49)]. The association between miRNAs and AF showed an OR of 2.51 [95% CI 1.99–3.16; I2 = 99%]. Our meta-analysis demonstrated that circulating miRNAs are potential biomarkers of AF, as they exhibit stable expression post–sample collection. In addition to regulating cellular processes, such as proliferation, differentiation, development, and cell death, miRNAs were found to be linked to arrhythmia development.
Maternal Pea Protein Intake Provides Sex-Specific Protection against Dyslipidemia in Offspring from Obese Pregnancies
Authors: Rideout Todd C. , Andreani Gabriella A. , Pembroke Jillian , Choudhary Divya , Browne Richard W. , Mahmood Saleh , Patel Mulchand S.
Published: February 2023

Increased consumption of dietary pulse protein has been shown to assist in body weight regulation and improve a range of metabolic health outcomes. We investigated if the exchange of casein for yellow pea protein (YPPN) in an obese-inducing maternal diet throughout pregnancy and lactation offered protection against obesity and dyslipidemia in offspring. Sixty female Sprague Dawley rats were fed a low-calorie control diet (CON), a high-caloric obesity-inducing diet (with casein protein (CP), HC-CP), or an isocaloric/macronutrient-matched HC diet supplemented with YPPN isolate (HC-PPN) in pre-pregnancy, gestation, and lactation. Body weight (BW) and metabolic outcomes were assessed in male and female offspring at weaning and in adulthood after consuming the CON diet in the postnatal period. Consumption of the HC-PPN diet did not protect against maternal obesity but did improve reproductive success compared with the HC-CP group (72.7% versus 43.7%) and reduced total energy, fat, and protein in maternal milk. Male, but not female, offspring from mothers fed the HC-CP diet demonstrated hyperphagia, obesity, dyslipidemia, and hepatic triglyceride (TG) accumulation as adults compared with CON offspring. Isocaloric exchange of CP for YPPN in a high-calorie obese-inducing diet did not protect against obesity but did improve several aspects of lipid metabolism in adult male offspring including serum total cholesterol, LDL/VLDL cholesterol, triglycerides (TGs), and hepatic TG concentration. Our results suggest that the exchange of CP for YPPN in a maternal obese-inducing diet selectively protects male offspring from the malprogramming of lipid metabolism in adulthood.
Intuitive Eating Behaviour among Young Malay Adults in Malaysian Higher Learning Institutions
Authors: Muhammad Rosmaliza , Ismail Wan Nur Diana Rajab aka Wan , Firdus Syauqina , Abdul Hamid Syahrul Bariah , Mohd Asmawi Ummi Mohlisi , Md Nor Norazmir
Published: February 2023

Despite the significance of dietary knowledge interventions, there is a lack of established studies on intuitive eating behaviour among young Malay adults in Malaysia. This cross-sectional study aimed to determine the intuitive eating score, identify the intuitive eating factors, and determine the association of intuitive eating with weight-control behaviours and binge eating. A total of 367 respondents completed self-administered questionnaires on sociodemographic characteristics, namely the Intuitive Eating Scale (IES-2) and The Diabetes Eating Problems Survey (DEPS). The findings reported IES-2 mean scores of 3.52 ± 0.32 and 3.47 ± 0.35 for both men and women. No difference in total IES-2 scores was found between genders for Unconditional Permission to Eat (UPE) and Reliance on Hunger and Satiety Cue (RHSC) subscales (p > 0.05). However, among all four subscales of IES-2, there was a gender difference in the mean EPR and B-FCC subscale scores (p < 0.05). A statistically significant difference was found in intuitive eating, which refers to a belief in one’s body’s ability to tell one how much to eat, in women across living areas (p < 0.05). The result shows that there is a relationship between weight-control behaviour and binge eating and dieting, with the coefficient of the relationship (R2) of 0.34. As a result, intuitive eating throughout young adulthood is likely to be related to a decreased prevalence of obesity, dieting, poor weight-management behaviours, and binge eating.
Acknowledgements upon Conclusion of the Pathogens Special Issue “Epidemiology, Surveillance and Control of Infectious Diseases”
Authors: Heuer Cord , Dunowska Magdalena
Published: February 2023

One Health is a popular headline for an all-inclusive concept of our scientific work [...]
Strong Association between Diarrhea and Concentration of Enterotoxigenic Escherichia coli Strain TW10722 in Stools of Experimentally Infected Volunteers
Authors: Vedøy Oda Barth , Steinsland Hans , Sakkestad Sunniva Todnem , Sommerfelt Halvor , Hanevik Kurt
Published: February 2023

Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of diarrheal illness in children and travelers in low- and middle-income countries. When volunteers are infected with ETEC strains, as part of experimental infection studies, some do not develop diarrhea. To improve our understanding of how these volunteers are protected, we investigated the association between stool ETEC DNA concentration, as determined by quantitative PCR, and the development and severity of disease in 21 volunteers who had been experimentally infected with ETEC strain TW10722. We found a strong association between maximum stool ETEC DNA concentration and the development of diarrhea: all of the 11 volunteers who did not develop diarrhea had <0.99% TW10722-specific DNA in their stools throughout the follow-up period of up to 9 days, while all of the 10 volunteers who did develop diarrhea had maximum DNA concentrations of ≥0.99%. Most likely, these maximum stool TW10722 DNA concentrations reflect the level of intestinal colonization and the risk of experiencing diarrhea, thereby, seems to be directly dependent on the level of colonization. Thus, the development and availability of vaccines and other prophylactic measures, even if they only partially reduce colonization, could be important in the effort to reduce the burden of ETEC diarrhea.
Genomic Epidemiology of Streptococcus pneumoniae Isolated in a Tertiary Hospital in Beijing, China, from 2018 to 2022
Authors: Fan Shuaihua , Duan Ning , Chen Wenjing , Zhao Xiuying , Wang Lijun , Du Pengcheng , Guo Jun
Published: February 2023

Streptococcus pneumoniae is one of the most common bacterial pathogens of a wide range of community-acquired infections. It has been more and more recognized that this bacterium could also play a role as a cause of nosocomial infections. In this study, by retrospective analysis of the phenotypic resistance characteristics and genomic characteristics of 52 S. pneumoniae isolates in a hospital in Beijing, China, from 2018 to 2022, we explored the carriage of resistance genes and mutations in penicillin-binding proteins corresponding to the resistances, and identified the population diversity based on the prediction of serotypes and identification of sequence types (STs). The isolates displayed resistances to erythromycin (98%), tetracycline (96%), sulfonamide (72%) and penicillin G (42%). Among the 52 isolates, 41 displayed multiple-drug resistance. In total, 37 STs and 21 serotypes were identified, and the clonal complex 271 serogroup 19 was the most prevalent subtype. Only 24 isolates (46.2%) of 7 serotypes were covered by the 13-valent pneumococcal conjugate vaccination. The isolates showed high carriages of resistance genes, including tet(M) (100%) and erm(B) (98.1%); additionally, 32 isolates (61.5%) had mutations in penicillin-binding proteins. We also observed 11 healthcare-associated infections and 3 cases infected by different subtypes of isolates. We did not find nosocomial transmissions between the patients, and these cases might be associated with the asymptomatic colonization of S. pneumoniae in the human population. Our results called for further active surveillance of these subtypes, as well as the continuous optimization of the treatment protocols.
Composting of Wild Boar Carcasses in Lithuania Leads to Inactivation of African Swine Fever Virus in Wintertime
Authors: Carrau Tessa , Malakauskas Alvydas , Masiulis Marius , Bušauskas Paulius , Japertas Sigitas , Blome Sandra , Deutschmann Paul , Friedrichs Virginia , Pileviečienė Simona , Dietze Klaas , Beltrán-Alcrudo Daniel , Hóvári Márk , Flory Gary A.
Published: February 2023

African swine fever (ASF) continues to spread and persist in the Eurasian wild boar population. The infection pressure resulting from infected carcasses in the environment can be a major contributor to disease persistence and spread. For this reason, it is crucial to find a safe and efficient method of carcass disposal under different circumstances. In the presented study, we investigated open-air composting of carcasses under winter conditions in northeastern Europe, i.e., Lithuania. We can demonstrate that the ASF virus (ASFV) is inactivated in both entire wild boar carcasses and pieces thereof in a time- and temperature-dependent manner. Composting piles reached up to 59.0 °C, and ASFV was shown to be inactivated. However, the ASFV genome was still present until the end of the 112-day sampling period. While further studies are needed to explore potential risk factors (and their mitigation), such as destruction of composting piles by scavengers or harsh weather conditions, composting seems to present a valid method to inactivate the ASFV in wild boar carcasses where rendering or other disposal methods are not feasible. In summary, composting provides a new tool in our toolbox of ASF control in wild boar and can be considered for carcass disposal.
Uncovering a Complex Virome Associated with the Cacao Pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata
Authors: Espinal Roy Bogardid Ardón , de Santana Sabrina Ferreira , Santos Vinícius Castro , Lizardo Gabriela Nicolle Ramos , Silva Raner José Santana , Corrêa Ronan Xavier , Loguercio Leandro Lopes , Góes-Neto Aristóteles , Pirovani Carlos Priminho , Fonseca Paula Luize Camargos , Aguiar Eric Roberto Guimarães Rocha
Published: February 2023

Theobroma cacao is one of the main crops of economic importance in the world as the source of raw material for producing chocolate and derivatives. The crop is the main source of income for thousands of small farmers, who produce more than 80% of the world’s cocoa supply. However, the emergence, re-emergence and proliferation of pathogens, such as Ceratocystis spp., the causative agent of Ceratocystis wilt disease and canker disease, have been affecting the sustainability of many crops. Fungal control is laborious, often depending on fungicides that are expensive and/or toxic to humans, prompting researchers to look for new solutions to counteract the proliferation of these pathogens, including the use of biological agents such as mycoviruses. In this study, we investigated the diversity of microorganisms associated with the T. cacao pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata with a focus on the virome using RNA sequencing data available in public databases. We used a comprehensive bioinformatics pipeline containing several steps for viral sequence enrichment and took advantage of an integrated assembly step composed of different assemblers followed by sequence similarity searches using NCBI nonredundant databases. Our strategy was able to identify four putative C. cacaofunesta viruses (hypovirus, sclerotimonavirus, alphapartitivirus and narnavirus) and six C. fimbriata viruses (three alphaendornaviruses, one victorivirus and two mitoviruses). All the viral sequences identified showed similarity to viral genomes in public databases only at the amino acid level, likely representing new viral species. Of note, we present the first report of viruses associated with the cacao pathogens C. cacaofunesta and C. fimbriata and the second report of viral species infecting members of the Ceratocystidaceae family. Our findings highlight the need for further prospective studies to uncover the real diversity of fungus-infecting viruses that can contribute to the development of new management strategies.
Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion
Authors: Jonker Annelize , Michel Anita L.
Published: February 2023

Domestic ruminant abortions due to infectious agents represent an important cause of economic losses in the agricultural industry. This study aimed to optimise and apply qPCR assays for detection of Chlamydiales in domestic ruminant abortion cases. Primers and probes for detection of the order Chlamydiales, Chlamydia abortus, Chlamydia pecorum, Parachlamydia acanthamoeba and Waddlia chondrophila were taken from the literature to create one singleplex and two duplex assays and the assays were optimised. Placentitis and pneumonia are pathological lesions associated with Chlamydiales infection. In a previous study, twenty-five clinical cases had pathological lesions of placentitis or pneumonia. These cases were investigated further by application of the qPCR assays in this study. Chlamydiales were detected in 16 cases. C. abortus, P. acanthamoeba and W. chondrophila were detected in bovine; and C. pecorum and W. chondrophila in ovine and caprine cases. Chlamydiales were detected in three previously inconclusive cases. Identification was improved from genus to species level (C. pecorum). Four cases remained inconclusive. In conclusion, detection of Chlamydiales and differentiation to species level was improved. This study reports the first detection of P. acanthamoeba and W. chondrophila in abortion cases in South Africa, indicating a potentially significant role in abortions in this country.
Addressing Microbial Resistance Worldwide: Challenges over Controlling Life-Threatening Fungal Infections
Authors: Martins-Santana Leonardo , Rezende Caroline Patini , Rossi Antonio , Martinez-Rossi Nilce Maria , Almeida Fausto
Published: February 2023

Fungal infections are a serious global concern because of their ability to spread and colonize host tissues in immunocompromised individuals. Such infections have been frequently reported worldwide and are currently gaining clinical research relevance owing to their resistant character, representing a bottleneck in treating affected people. Resistant fungi are an emergent public health threat. The upsurge of such pathogens has led to new research toward unraveling the destructive potential evoked by these species. Some fungi—grouped into Candida, Aspergillus, and Cryptococcus—are causative agents of severe and systemic infections. They are associated with high mortality rates and have recently been described as sources of coinfection in COVID-hospitalized patients. Despite the efforts to elucidate the challenges of colonization, dissemination, and infection severity, the immunopathogenesis of fungal diseases remains a pivotal characteristic in fungal burden elimination. The struggle between the host immune system and the physiological strategies of the fungi to maintain cellular viability is complex. In this brief review, we highlight the relevance of drug resistance phenotypes in fungi of clinical significance, taking into consideration their physiopathology and how the scientific community could orchestrate their efforts to avoid fungal infection dissemination and deaths.
Cisplatin-Loaded Thermosensitive Liposomes Functionalized with Hyaluronic Acid: Cytotoxicity and In Vivo Acute Toxicity Evaluation
Authors: Gomes Isabela Pereira , Silva Juliana de Oliveira , Cassali Geovanni Dantas , De Barros André Luís Branco , Leite Elaine Amaral
Published: February 2023

Cisplatin (CDDP) is a potent antitumor drug used in first-line chemotherapy against several solid tumors, including breast cancer. However, toxicities and drug resistance limit its clinical application. Thermosensitive liposome (TSL) functionalized with hyaluronic acid (HA) containing cisplatin (TSL-CDDP-HA) was developed by our research group aiming to promote the release of CDDP in the tumor region under hyperthermia conditions, as well as to decrease toxicity. Thus, this study aimed to evaluate this new formulation (HA-coated TSL-CDDP) concerning in vitro behavior and in vivo toxicity compared to non-coated TSL-CDDP and free CDDP. Cytotoxicity assays and nuclear morphology were carried out against triple-negative breast cancer cells (MDA-MB-231), while an in vivo toxicity study was performed using healthy Swiss mice. The results showed an increase (around 3-fold) in cytotoxicity of the cationic formulation (non-coated TSL-CDDP) compared to free CDDP. On the other hand, TSL-CDDP treatment induced the appearance of 2.5-fold more senescent cells with alteration of nuclear morphology than the free drug after hyperthermia condition. Furthermore, the association of liposomal formulations treatment with hyperthermia increased the percentage of apoptotic cells compared to those without heating. The percentage of apoptotic cells was 1.7-fold higher for TSL-CDDP-HA than for TSL-CDDP. For the in vivo toxicity data, the TSL-CDDP treatment was also toxic to healthy cells, inducing nephrotoxicity with a significant increase in urea levels compared to the saline control group (73.1 ± 2.4 vs. 49.2 ± 2.8 mg/mL). On the other hand, the HA-coated TSL-CDDP eliminated the damages related to the use of CDDP since the animals did not show changes in hematological and biochemical examinations and histological analyses. Thus, data suggest that this new formulation is a potential candidate for the intravenous therapy of solid tumors.
Semantic Analysis of the Philosophical Discourse of the Transhumanism Concept in the Works of Russian Scholars
Authors: Rozhkov Alexandr , Gura Alena , Arutyunyan Margarita
Published: February 2023

The purpose of this research paper is to semantically analyze the concept of transhumanism in the publications of Russian scientists, as well as to study the influence of the idea of transhumanism as the leading philosophy of human improvement on the global differentiation of the world through a comparative analysis of the level of life expectancy in the Russian Federation, the USA, and China. Findings indicate that, in general, when setting the right goals based on the Russian cosmism and transhumanism concepts can and should bring certain positive benefits to humanity. First of all, this involves treatment of incurable and complex diseases and enhancement of human capabilities in space expansion. The scientific contribution and practical significance of the research lie in the development of three exponential models of life expectancy growth in accordance with the indicator of economic development that is based on the gross domestic product per capita level.
Observation of Giant Angular Goos-Hanchen Shifts Enhanced by Surface Plasmon Resonance in Subwavelength Grating
Authors: Petrov Nikolai I. , Sokolov Yuri M. , Stoiakin Vladimir V. , Danilov Viktor A. , Popov Vladimir V. , Usievich Boris A.
Published: February 2023

The effect of the Goos-Hanchen (GH) angular shift for a visible light beam under the excitation of surface plasmon resonance (SPR) in a metal subwavelength grating has been investigated. Extremely large angular GH shifts have been demonstrated for a subwavelength grating with an optimal depth. The high sensitivity of the beam shape transformation and the GH shift to a change in the angle of incidence near the SPR has been shown by rigorous electromagnetic simulation and demonstrated experimentally. The focusing of the reflected beam near the subwavelength grating surface has been demonstrated.
A Silicon-Based On-Chip 64-Channel Hybrid Wavelength- and Mode-Division (de)Multiplexer
Authors: Yin Yuxiang , Yu Hang , Tu Donghe , Huang Xingrui , Yu Zhiguo , Guan Huan , Li Zhiyong
Published: February 2023

An on-chip 64-channel hybrid (de)multiplexer for wavelength-division multiplexing (WDM) and mode-division multiplexing (MDM) is designed and demonstrated on a 220 nm SOI platform for the demands of large capacity optical interconnections. The designed hybrid (de)multiplexer includes a 4-channel mode (de)multiplexer and 16-channel wavelength-division (de)multiplexers. The mode (de)multiplexer is comprised of cascaded asymmetric directional couplers supporting coupling between fundamental TE mode and higher-order modes with low crosstalks in a wide wavelength range. The wavelength-division (de)multiplexers consist of two bi-directional micro-ring resonator arrays for four 16-channel WDM signals. Micro-heaters are placed on the micro-resonators for thermal tuning. According to the experimental results, the excess loss is <3.9 dB in one free spectral range from 1522 nm to 1552 nm and <5.6 dB in three free spectral ranges from 1493 nm to 1583 nm. The intermode crosstalks are −23.2 dB to −33.2 dB, and the isolations between adjacent and nonadjacent wavelength channels are about −17.1 dB and −22.3 dB, respectively. The thermal tuning efficiency is ∼2.22 mW/nm over one free spectral range.
Orchard Management and Incorporation of Biochemical and Molecular Strategies for Improving Drought Tolerance in Fruit Tree Crops
Authors: Devin Sama Rahimi , Prudencio Ángela S. , Mahdavi Sayyed Mohammad Ehsan , Rubio Manuel , Martínez-García Pedro J. , Martínez-Gómez Pedro
Published: February 2023

Water scarcity is one of the greatest concerns for agronomy worldwide. In recent years, many water resources have been depleted due to multiple factors, especially mismanagement. Water resource shortages lead to cropland expansion, which likely influences climate change and affects global agriculture, especially horticultural crops. Fruit yield is the final aim in commercial orchards; however, drought can slow tree growth and/or decrease fruit yield and quality. It is therefore necessary to find approaches to solve this problem. The main objective of this review is to discuss the most recent horticultural, biochemical, and molecular strategies adopted to improve the response of temperate fruit crops to water stress. We also address the viability of cultivating fruit trees in dry areas and provide precise protection methods for planting fruit trees in arid lands. We review the main factors involved in planting fruit trees in dry areas, including plant material selection, regulated deficit irrigation (DI) strategies, rainwater harvesting (RWH), and anti-water stress materials. We also provide a detailed analysis of the molecular strategies developed to combat drought, such as Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) through gene overexpression or gene silencing. Finally, we look at the molecular mechanisms associated with the contribution of the microbiome to improving plant responses to drought.
Syntenic Cell Wall QTLs as Versatile Breeding Tools: Intraspecific Allelic Variability and Predictability of Biomass Quality Loci in Target Plant Species
Authors: Pancaldi Francesco , van Loo Eibertus N. , Senio Sylwia , Al Hassan Mohamad , van der Cruijsen Kasper , Paulo Maria-João , Dolstra Oene , Schranz M. Eric , Trindade Luisa M.
Published: February 2023

Syntenic cell wall QTLs (SQTLs) can identify genetic determinants of biomass traits in understudied species based on results from model crops. However, their effective use in plant breeding requires SQTLs to display intraspecific allelic variability and to predict causative loci in other populations/species than the ones used for SQTLs identification. In this study, genome assemblies from different accessions of Arabidopsis, rapeseed, tomato, rice, Brachypodium and maize were used to evaluate the intraspecific variability of SQTLs. In parallel, a genome-wide association study (GWAS) on cell wall quality traits was performed in miscanthus to verify the colocalization between GWAS loci and miscanthus SQTLs. Finally, an analogous approach was applied on a set of switchgrass cell wall QTLs retrieved from the literature. These analyses revealed large SQTLs intraspecific genetic variability, ranging from presence–absence gene variation to SNPs/INDELs and changes in coded proteins. Cell wall genes displaying gene dosage regulation, such as PAL and CAD, displayed presence–absence variation in Brachypodium and rapeseed, while protein INDELs were detected for the Brachypodium homologs of the rice brittle culm-like 8 locus, which may likely impact cell wall quality. Furthermore, SQTLs significantly colocalized with the miscanthus and switchgrass QTLs, with relevant cell wall genes being retained in colocalizing regions. Overall, SQTLs are useful tools to screen germplasm for relevant genes and alleles to improve biomass quality and can increase the efficiency of plant breeding in understudied biomass crops.
Diagnosis and Mobile Application of Apple Leaf Disease Degree Based on a Small-Sample Dataset
Authors: Li Lili , Wang Bin , Li Yanwen , Yang Hua
Published: February 2023

The accurate segmentation of apple leaf disease spots is the key to identifying the classification of apple leaf diseases and disease severity. Therefore, a DeepLabV3+ semantic segmentation network model with an actors spatial pyramid pool module (ASPP) was proposed to achieve effective extraction of apple leaf lesion features and to improve the apple leaf disease recognition and disease severity diagnosis compared with the classical semantic segmentation network models PSPNet and GCNet. In addition, the effects of the learning rate, optimizer, and backbone network on the performance of the DeepLabV3+ network model with the best performance were analyzed. The experimental results show that the mean pixel accuracy (MPA) and mean intersection over union (MIoU) of the model reached 97.26% and 83.85%, respectively. After being deployed into the smartphone platform, the detection time of the detection system was 9s per image for the portable and intelligent diagnostics of apple leaf diseases. The transfer learning method provided the possibility of quickly acquiring a high-performance model under the condition of small datasets. The research results can provide a precise guide for the prevention and precise control of apple diseases in fields.
Illumina RNA and SMRT Sequencing Reveals the Mechanism of Uptake and Transformation of Selenium Nanoparticles in Soybean Seedlings
Authors: Xiong Yuzhou , Xiang Xumin , Xiao Chunmei , Zhang Na , Cheng Hua , Rao Shen , Cheng Shuiyuan , Li Li
Published: February 2023

Selenium (Se) is an essential element for mammals, and its deficiency in the diet is a global problem. Agronomic biofortification through exogenous Se provides a valuable strategy to enhance human Se intake. Selenium nanoparticles (SeNPs) have been regarded to be higher bioavailability and less toxicity in comparison with selenite and selenate. Still, little has been known about the mechanism of their metabolism in plants. Soybean (Glycine max L.) can enrich Se, providing an ideal carrier for Se biofortification. In this study, soybean sprouts were treated with SeNPs, and a combination of next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing was applied to clarify the underlying molecular mechanism of SeNPs metabolism. A total of 74,662 nonredundant transcripts were obtained, and 2109 transcription factors, 9687 alternative splice events, and 3309 long non-coding RNAs (lncRNAs) were predicted, respectively. KEGG enrichment analysis of the DEGs revealed that metabolic pathways, biosynthesis of secondary metabolites, and peroxisome were most enriched both in roots and leaves after exposure to SeNPs. A total of 117 transcripts were identified to be putatively involved in SeNPs transport and biotransformation in soybean. The top six hub genes and their closely coexpressed Se metabolism-related genes, such as adenylylsulfate reductase (APR3), methionine-tRNA ligase (SYM), and chloroplastic Nifs-like cysteine desulfurases (CNIF1), were screened by WGCNA and identified to play crucial roles in SeNPs accumulation and tolerance in soybean. Finally, a putative metabolism pathway of SeNPs in soybean was proposed. These findings have provided a theoretical foundation for future elucidation of the mechanism of SeNPs metabolism in plants.
Epidemiology and Control of Plant Diseases
Authors: Vitale Alessandro
Published: February 2023

I am pleased to present this edition of the Special Issue of Plants, dedicated to multifaceted topic of epidemiology and control of plant diseases in agricultural systems [...]
Positive Effect Induced by Plasma Treatment of Seeds on the Agricultural Performance of Sunflower
Authors: Florescu Ioana , Radu Ioan , Teodoru Andrei , Gurau Lorena , Chireceanu Constantina , Bilea Florin , Magureanu Monica
Published: February 2023

The need for efficient technologies to enhance productivity in agriculture strongly motivates research on plasma treatment of seeds and plants. In this study, the influence of plasma treatment on sunflower (Helianthus annuus L.) seeds was evidenced throughout the entire life span of the plants. The seeds were packed in a DBD reactor operated in air and treated in plasma for 10 min, using a sinusoidal voltage of 16 kV amplitude at 50 Hz frequency. Early growth observation of plants under laboratory conditions showed that, after a slower start, the plasma-treated seeds developed faster and produced taller seedlings with greater total mass as compared to the control samples. Results obtained from mature plants cultivated in the field revealed a positive effect of plasma exposure with respect to capitulum size, number of seeds per capitulum and mass per thousand seeds, resulting in a remarkable increase in crop yield. The plasma effect lasted for at least two weeks of seed storage; however, it was considerably affected by the sowing period.
Localization of Lipid Droplets in Embryonic Axis Radicle Cells of Soybean Seeds under Various Imbibition Regimes Indicates Their Role in Desiccation Tolerance
Authors: Khanam Salma , Atsuzawa Kimie , Kaneko Yasuko
Published: February 2023

Desiccation tolerance allows plant seeds to remain viable during desiccation and subsequent re-hydration. In this study, we tried to develop an experimental system to understand the difference between desiccation tolerant and desiccation sensitive radicle cells by examining excised embryonic axes after re-desiccation and subsequent imbibition under various regimes. Embryonic axes excised from soybean (Glycine max (L.) Merr.) seeds imbibed for 3 h to 15 h which remained attached to the cotyledons during imbibition would grow normally after 24 h of desiccation and re-imbibition on wet filter paper. By contrast, when the embryonic axes excised after 3 h imbibition of seeds were kept on wet filter paper for 12 h to 16 h, their growth was significantly retarded after 24 h of desiccation and subsequent re-imbibition. Numerous lipid droplets were observed lining the plasma membrane and tonoplasts in radicle cells of desiccation tolerant embryonic axes before and after desiccation treatment. By contrast, the lipid droplets lining the plasma membrane and tonoplasts became very sparse in radicle cells that were placed for longer times on wet filter paper before desiccation. We observed a clear correlation between the amount of lipid droplets lining plasma membranes and the ability to grow after desiccation and re-imbibition of the excised embryonic axes. In addition to the reduction of lipid droplets in the cells, a gradual increase in starch grains was observed. Large starch grains accumulated in the radicle cells of those axes that failed to grow further.
Energy Consumption vs. Tensile Strength of Poly[methyl methacrylate] in Material Extrusion 3D Printing: The Impact of Six Control Settings
Authors: Vidakis Nectarios , Petousis Markos , Mountakis Nikolaos , Moutsopoulou Amalia , Karapidakis Emmanuel
Published: February 2023

The energy efficiency of material extrusion additive manufacturing has a significant impact on the economics and environmental footprint of the process. Control parameters that ensure 3D-printed functional products of premium quality and mechanical strength are an established market-driven requirement. To accomplish multiple objectives is challenging, especially for multi-purpose industrial polymers, such as the Poly[methyl methacrylate]. The current paper explores the contribution of six generic control factors (infill density, raster deposition angle, nozzle temperature, print speed, layer thickness, and bed temperature) to the energy performance of Poly[methyl methacrylate] over its mechanical performance. A five-level L25 Taguchi orthogonal array was composed, with five replicas, involving 135 experiments. The 3D printing time and the electrical consumption were documented with the stopwatch approach. The tensile strength, modulus, and toughness were experimentally obtained. The raster deposition angle and the printing speed were the first and second most influential control parameters on tensile strength. Layer thickness and printing speed were the corresponding ones for the energy consumption. Quadratic regression model equations for each response metric over the six control parameters were compiled and validated. Thus, the best compromise between energy efficiency and mechanical strength is achievable, and a tool creates significant value for engineering applications.
Biomaterials in Medical Applications
Authors: Wang Hsiuying
Published: February 2023

Natural biomaterials are materials extracted from living organisms or their by-products [...]
Mock-Ups in Plastic Conservation Research: Processing and Aging of 3D Celluloid Specimens Simulating Historical Objects
Authors: Elsässer Christina , Mayr Veronika , Montag Peter , Angelin Eva Mariasole , Hilbig Harald , Grosse Christian Ulrich , Pamplona Marisa
Published: February 2023

The preparation of mock-ups in heritage science studies represents a valid alternative for investigation purposes, avoiding extensive sampling of cultural heritage objects. This work presents for the first time the successful preparation of three dimensional (3D) mock-ups made of celluloid, considering a combination of historical industrial production strategies and small-scale lab facilities. Prefabricated transparent celluloid sheets were acquired and then shaped through compression molding for creating mock-ups with 3D geometries. These reflected common and representative shapes encountered in the collection of the Deutsches Museum. Visual inspection of the mock-ups allowed determining the best compression molding conditions. Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) confirmed the absence of molecular heterogeneity due to the processing method. Artificial aging of the mock-ups was conducted to reach degradation states comparable with naturally aged objects. ATR-FTIR investigation offered first insights into the induced artificial degradation. Ion chromatography (IC) and gel permeation chromatography (GPC) analyses allowed to assess the extent of the artificial aging of the celluloid mock-ups and confirmed the occurrence of loss of camphor, denitration, and main chain polymer scission, the latter being the predominant decay path. The comparison with historical objects highlighted that the mock-ups are representative of moderately aged artifacts. As such, this study paves the way for implementing moderately aged celluloid 3D mock-ups in heritage science research, enabling in-depth testing for the scope of conservation.
Molecular Dynamic and Dissipative Particle Dynamic Simulation on the Miscibility of NR/CR Blends
Authors: Ma Yanbin , Yuan Xiaoqin , Jiang Ruifeng , Liao Jianhe , Yu Rentong , Chen Yongping , Liao Lusheng
Published: February 2023

Natural rubber (NR) exhibits good elasticity, flexural resistance, wear resistance, and excellent mechanical properties, and it has been widely used in aerospace, transportation, medical, and health fields. For NR, however, the resistance to thermal-oxidation and ozone aging is fairly poor. Although aging properties of NR can be significantly improved with the incorporation of chloroprene rubber (CR) according to some references, the miscibility between NR and CR, the morphologies of the binary blends, and so on are revealed ambiguously. In this work, molecular dynamics simulation (MD) and dissipative particle dynamics (DPD) simulation were carried out to predict the compatibility between natural rubber and chloroprene rubber in view of Flory–Huggins parameters. The morphologies of the blends were obtained with the use of the DPD method. The simulation results were furtherly examined by means of Fourier transform infrared spectroscopy (FT-IR) and dynamic mechanical analysis (DMA). It was found that the miscibility between NR and CR is poor. Nevertheless, the miscibility could be improved when the content of CR is 50% or 90%. In addition, spinodal decomposition with a critical temperature of 390 K would take place according to the phase diagram. Microphase structure such as spherical, lamellar, and bicontinuous phases can be found with different contents of CR in the blends with the results of morphologies analysis.
Curing, Properties and EMI Absorption Shielding of Rubber Composites Based on Ferrites and Carbon Fibres
Authors: Kruželák Ján , Kvasničáková Andrea , Džuganová Michaela , Hašková Lenka , Dosoudil Rastislav , Hudec Ivan
Published: February 2023

In this work, magnetic soft ferrites, namely manganese–zinc ferrite, nickel–zinc ferrite and combinations of both fillers, were incorporated into acrylonitrile-butadiene rubber to fabricate composite materials. The total content of ferrites was kept constant—300 phr. The second series of composites was fabricated with a similar composition. Moreover, carbon fibres were incorporated into rubber compounds in constant amount—25 phr. The work was focused on investigation of the fillers on absorption shieling performance of the composites, which was investigated within the frequency range 1–6 GHz. Then, the physical–mechanical properties of the composites were evaluated. The achieved results demonstrated that the absorption shielding efficiency of both composite types increased with increasing proportion of nickel–zinc ferrite, which suggests that nickel–zinc ferrite demonstrated better absorption shielding potential. Higher electrical conductivity and higher permittivity of composites filled with carbon fibres and ferrites resulted in their lower absorption shielding performance. Simultaneously, they absorbed electromagnetic radiation at lower frequencies. On the other hand, carbon fibres reinforced the rubber matrix, and subsequent improvement in physical–mechanical properties was recorded.
Expected Impact of Industry 4.0 on Employment in Selected Professions in the Czech Republic and Germany
Authors: Milichovský František , Kuba Karel
Published: February 2023

The topic of Industry 4.0 is more actual for various companies worldwide. Its impact is anywhere in company and government areas. Due to the individual parts of Industry 4.0, such as digitalization and robotization, we express changes impact on human resource management, where the most changes are defined. This contribution is focused on human resource management in the context of the application of Industry 4.0 in engineering companies operating in the Czech Republic and Germany. The main objective of the paper is to define potential connections between Industry 4.0 and its areas with the forfeiture of professions and preparedness for potential job changes. We employed a primary research approach with in-depth interviews and a questionnaire survey to reach a defined goal. The interviews were aimed at top managers and a questionnaire survey of ordinary employees and students/temporary workers. According to the gained results, there exist relevant statistical dependencies between Industry 4.0 knowledge (including its parts) and up-to-date situations in the companies in the Czech Republic and Germany.
A Quantitative Analysis of Chemical Plant Safety Based on Bayesian Network
Authors: Song Qiusheng , Song Li
Published: February 2023

Once a chemical production accident occurs in a chemical plant, it often causes serious economic losses, casualties, and environmental damage. Statistics show that many major accidents in the production and storage of chemicals are mainly caused by human factors. This article considers the influence of the human factor and proposes a quantitative analysis model of a chemical plant based on a Bayesian network. The model takes into account the main human factors in seven aspects: organization, information, job design, human system interface, task environment, workplace design, and operator characteristics. The Bayesian network modeling method and simulation were used to predict the safety quantitative value and safety level of the chemical plant. Using this model, we can quickly calculate the safe quantitative ratio of each factor in the chemical plant. Through the safety quantitative value, safety level, and sensitivity analysis, the safety hazards of chemical companies can be discovered. Immediate improvements of potential safety hazards in chemical plants are very effective in preventing major safety accidents. This model provides an effective method for chemical park managers to monitor and manage chemical plants based on quantitative safety data.
Adaptive Coexistence of Synchronization and Anti-Synchronization for a Class of Switched Chaotic Systems
Authors: Ren Ling , Peng Chenchen
Published: February 2023

This paper addresses the problem of coexistence of synchronization and anti-synchronization (CSAS) for a class of switched chaotic systems by adaptive control method, where the switched system is realized by unified chaotic systems under arbitrary switching signal. Firstly, necessary and sufficient conditions for the CSAS of the chaotic systems are proposed from two perspectives, one is by analyzing the parity of the system expression, and the other is by decomposing the system. Secondly, according to the obtained necessary and sufficient conditions, two algorithms are given to search the synchronization variables and anti-synchronization variables in the chaotic systems. Thirdly, the CSAS of the switched chaotic system can be achieved by a designed adaptive global controller with only one input channel under the arbitrary switching signal. Finally, the numerical simulation results verify the validity and effectiveness of the method we obtained.
Thermal Stress Analysis of Blast Furnace Hearth with Typical Erosion Based on Thermal Fluid-Solid Coupling
Authors: Wang Lei , Chen Liangyu , Yuan Fei , Zhao Lei , Li Yang , Ma Jiaocheng
Published: February 2023

The life of the hearth is the main limiting link of the campaign of a blast furnace. As the equipment for holding molten iron in the furnace, the high-temperature molten iron is in direct contact with the refractory, which makes the refractory have a larger temperature increase. If the temperature gradient inside the refractory is large, it generates large thermal stress and causes the refractory to crack. Blast furnace gas and molten iron intrude into the gap, which directly causes melting erosion and other chemical erosion with carbon bricks. It aggravates the erosion degree of the furnace and seriously affects the production life of the furnace. Therefore, the furnace often occurs with different types of severe depression erosion in the late service of the blast furnace. In this study, the calculation model of the thermal fluid-solid coupling considering the molten iron flow and the solidification of molten iron was established. This calculation model was applied to study thermal stresses in the furnace with severe erosion. Based on the calculation model, the effect of blast furnace production parameters and deadman condition on thermal stresses in the furnace with severe depression erosion were analyzed, including tapping productivity, tapping temperature, cooling intensity, and deadman geometry. The research results are of great significance for prolonging the safe production life of blast furnaces.
Magneto-Thermal Coupling Simulation of Flowing Liquid Induction Heating through Static Mixer-Type Susceptors
Authors: Shi Mingxuan , Xu Qing , Li Yanhua , Deng Lisheng , Dai Xiaoyong
Published: February 2023

As a new non-contact heating technology, induction heating technology has very broad application prospects in the field of fluid food heating. However, its application is inevitably affected by the heat concentration caused by uneven energy distribution. The uneven temperature distribution of the heating process will lead to the decrease in the quality of heating products. Therefore, based on the previous research, in order to improve the uniformity of heat distribution in the heating process, this study selected the susceptor with the greatest potential for efficient and the most uniform heating fluid to carry out the coupling simulation of electromagnetic heat transfer. The susceptor was simulated and optimized in three aspects: different power comparisons, the influence of structural change on temperature distribution uniformity, and the influence of physical property change of metal material on temperature distribution uniformity. The results show that the simulation results are in good agreement with the experimental results, and the error between the experimental and simulation values of the outlet temperature at Kelvin temperature is less than 0.18%. The change of geometric structure had a great influence on the uniformity of temperature distribution, and the uniformity of temperature distribution was inversely proportional to the conductivity. During the simulation, the temperature of the fluid heated by the susceptor was increased from 284.75 K to about 333K. The temperature distribution of the fluid at the outlet of the susceptor was uniform, and the temperature difference was about 1 K.