bluuebunny/crossref_metadata_2025 huggingface dataset:
Overexpressed KCNK1 regulates potassium channels affecting molecular mechanisms and biological pathways in bladder cancer
Authors: Zhang Wei , Chen Xiao-Song , Wei Ying , Wang Xiao-Min , Chen Xian-Jin , Chi Bang-Teng , Huang Lin-Qing , He Rong-Quan , Huang Zhi-Guang , Li Qi , Chen Gang , He Juan , Wu Mei
Published: April 2024

Background

This study aimed to explore the expression, molecular mechanism and its biological function of potassium two pore domain channel subfamily K member 1 (KCNK1) in bladder cancer (BC).

Methods

We integrated large numbers of external samples (n = 1486) to assess KCNK1 mRNA expression levels and collected in-house samples (n = 245) for immunohistochemistry (IHC) experiments to validate at the KCNK1 protein level. Single-cell RNA sequencing (scRNA-seq) analysis was performed to further assess KCNK1 expression and cellular communication. The transcriptional regulatory mechanisms of KCNK1 expression were explored by ChIP-seq, ATAC-seq and ChIA-PET data. Highly expressed co-expressed genes (HECEGs) of KCNK1 were used to explore potential signalling pathways. Furthermore, the immunoassay, clinical significance and molecular docking of KCNK1 were calculated.

Results

KCNK1 mRNA was significantly overexpressed in BC (SMD = 0.58, 95% CI [0.05; 1.11]), validated at the protein level (p < 0.0001). Upregulated KCNK1 mRNA exhibited highly distinguishing ability between BC and control samples (AUC = 0.82 [0.78–0.85]). Further, scRNA-seq analysis revealed that KCNK1 expression was predominantly clustered in BC epithelial cells and tended to increase with cellular differentiation. BC epithelial cells were involved in cellular communication mainly through the MK signalling pathway. Secondly, the KCNK1 transcription start site (TSS) showed promoter-enhancer interactions in three-dimensional space, while being transcriptionally regulated by GRHL2 and FOXA1. Most of the KCNK1 HECEGs were enriched in cell cycle–related signalling pathways. KCNK1 was mainly involved in cellular metabolism–related pathways and regulated cell membrane potassium channel activity. KCNK1 expression was associated with the level of infiltration of various immune cells. Immunotherapy and chemotherapy (docetaxel, paclitaxel and vinblastine) were more effective in BC patients in the high KCNK1 expression group. KCNK1 expression correlated with age, pathology grade and pathologic_M in BC patients.

Conclusions

KCNK1 was significantly overexpressed in BC. A complex and sophisticated three-dimensional spatial transcriptional regulatory network existed in the KCNK1 TSS and promoted the upregulated of KCNK1 expression. The high expression of KCNK1 might be involved in the cell cycle, cellular metabolism, and tumour microenvironment through the regulation of potassium channels, and ultimately contributed to the deterioration of BC.
The effect of preoperative TSH levels on perioperative complications in patients undergoing pituitary surgery
Authors: Kirbas Gizem , Ozkalkanli Murat Yasar , Tuzen Ahmet Salih
Published: April 2024

Introduction

Pituitary surgery involves special conditions for the anaesthetist due to the anatomical localisation and the role of the pituitary gland in hormonal balance. The aim of this study was to retrospectively investigate the effect of TSH levels on perioperative complication rates in patients undergoing pituitary surgery.

Methods

In this study, patients who underwent pituitary surgery at Izmir Katip Celebi University Ataturk Training and Research Hospital between January 2017 and November 2022 were retrospectively screened. Two patients out of the 137 were excluded from the study as they underwent simultaneous aneurysm surgery along with pituitary tumor surgery. A total of 135 patients meeting the criteria were analyzed. Patients were divided into three groups according to TSH levels. Perioperative complication rates and mortality rates were compared between the three groups.

Findings

The study included a total of 135 patients, with an age range of 16–76 years. Among the groups with low–normal–high TSH levels, there were no statistically significant difference observed, in postoperative complications, such as epistaxis, rhinorrhea, postoperative nausea/vomiting, seizures, hypertension, and hypotension. When looking at the incidence of postoperative diabetes insipidus, a significant difference was found between the groups. When examining the mortality rates on the 28th day, a significant difference was found between the groups, with a mortality rate of 25% in the high TSH group (p < 0.5).

Conclusions

The risk of perioperative mortality is higher in patients with TSH levels above normal.
Aptamer-based protein molecule detection via cyclic reverse transcription coupling with self-priming hairpin-triggered CRISPR-Cas12a system
Authors: Gao Jie , Yang Xiaoqing
Published: April 2024

Protein biomarkers (e.g. thrombin) are of great significance for the biological process of the organism, and its aberrant expression is closely associated with the development of diseases. With thrombin, a serine protease that plays a crucial role in maintaining homeostasis and promoting blood clotting, as detection target, this study introduces a novel approach for sensitive and accurate measurement of protein biomarker expression by utilization of cyclic reverse transcription (CRT) in combination with the self-priming hairpin-triggered CRISPR-Cas12a system. In this method, an elegantly designed sensing probe is utilized to specifically bind with the thrombin protein and convert the protein signals to nucleic acids signals, following by the CRT and CRISPR-Cas12a system-based signal amplification strategy. Taking the merit of the two-stage amplification, this assay has the capability to detect thrombin at the fM level. In addition, due to the aptamer sequence’s strong selectivity to thrombin protein and the dual-check process in the signal amplification process (first in the CRT and second in the CRISPR system), the proposed test demonstrates exceptional specificity in detecting thrombin. By re-designing the sensing probe, the established method could be extended to various protein biomarker detection. Ultimately, this assay has successfully enabled the accurate evaluation of biomarker levels in constructed clinical samples, showing significant potential for application in the realm of clinical molecular diagnosis.
Heat transport process associated with the 2021 eruption of Aso volcano revealed by thermal and gas monitoring
Authors: Narita Shohei , Yokoo Akihiko , Ohkura Takahiro , Morita Masaaki , Mori Toshiya , Yoshikawa Shin
Published: April 2024

The thermal activity of a magmatic–hydrothermal system commonly changes at various stages of volcanic activity. Few studies have provided an entire picture of the thermal activity of such a system over an eruptive cycle, which is essential for understanding the subsurface heat transport process that culminates in an eruption. This study quantitatively evaluated a sequence of thermal activity associated with two phreatic eruptions in 2021 at Aso volcano. We estimated plume-laden heat discharge rates and corresponding H2O flux during 2020–2022 by using two simple methods. We then validated the estimated H2O flux by comparison with volcanic gas monitoring results. Our results showed that the heat discharge rate varied substantially throughout the eruptive cycle. During the pre-eruptive quiescent period (June 2020–May 2021), anomalously large heat discharge (300–800 MW) were observed that were likely due to enhanced magma convection degassing. During the run-up period (June–October 2021), there was no evident change in heat discharge (300–500 MW), but this was accompanied by simultaneous pressurization and heating of an underlying hydrothermal system. These signals imply progress of partial sealing of the hydrothermal system. In the co-eruptive period, the subsequent heat supply from a magmatic region resulted in additional pressurization, which led to the first eruption (October 14, 2021). The heat discharge rates peaked (2000–4000 MW) the day before the second eruption (October 19, 2021), which was accompanied by sustained pressurization of the magma chamber that eventually resulted in a more explosive eruption. In the post-eruptive period, enhanced heat discharge (~ 1000 MW) continued for four months, and finally returned to the background level of the quiescent period (< 300 MW) in early March 2022. Despite using simple models, we quantitatively tracked transient thermal activity and revealed the underlying heat transport processes throughout the Aso 2021 eruptive activity.

Graphical abstract
Control of astrocytic Ca2+ signaling by nitric oxide-dependent S-nitrosylation of Ca2+ homeostasis modulator 1 channels
Authors: Puebla Mariela , Muñoz Manuel F. , Lillo Mauricio A. , Contreras Jorge E. , Figueroa Xavier F.
Published: April 2024

Background

Astrocytes Ca2+ signaling play a central role in the modulation of neuronal function. Activation of metabotropic glutamate receptors (mGluR) by glutamate released during an increase in synaptic activity triggers coordinated Ca2+ signals in astrocytes. Importantly, astrocytes express the Ca2+-dependent nitric oxide (NO)-synthetizing enzymes eNOS and nNOS, which might contribute to the Ca2+ signals by triggering Ca2+ influx or ATP release through the activation of connexin 43 (Cx43) hemichannels, pannexin-1 (Panx-1) channels or Ca2+ homeostasis modulator 1 (CALHM1) channels. Hence, we aim to evaluate the participation of NO in the astrocytic Ca2+ signaling initiated by stimulation of mGluR in primary cultures of astrocytes from rat brain cortex.

Results

Astrocytes were stimulated with glutamate or t-ACPD and NO-dependent changes in [Ca2+]i and ATP release were evaluated. In addition, the activity of Cx43 hemichannels, Panx-1 channels and CALHM1 channels was also analyzed. The expression of Cx43, Panx-1 and CALHM1 in astrocytes was confirmed by immunofluorescence analysis and both glutamate and t-ACPD induced NO-mediated activation of CALHM1 channels via direct S-nitrosylation, which was further confirmed by assessing CALHM1-mediated current using the two-electrode voltage clamp technique in Xenopus oocytes. Pharmacological blockade or siRNA-mediated inhibition of CALHM1 expression revealed that the opening of these channels provides a pathway for ATP release and the subsequent purinergic receptor-dependent activation of Cx43 hemichannels and Panx-1 channels, which further contributes to the astrocytic Ca2+ signaling.

Conclusions

Our findings demonstrate that activation of CALHM1 channels through NO-mediated S-nitrosylation in astrocytes in vitro is critical for the generation of glutamate-initiated astrocytic Ca2+ signaling.
Policy perception, job satisfaction and intentions to remain in rural area: evidence from the National Compulsory Service Programme in China
Authors: He Yanrong , Wang Peicheng , Du Yanrong , Li Hange , Chen Yanhua , Zhu Jiming
Published: April 2024

Background

Exploring factors that may influence general practitioners (GPs)’ intentions to remain in rural area is necessary to inform the training and placement of future medical workforce in rural area. However, little is known about how GPs’ perception towards the National Compulsory Service Programme (NCSP) and job satisfaction impact their turnover intention. This paper explores GPs’ intentions to remain in rural China and how their policy perception and job satisfaction predict the intentions.

Methods

We conducted a cross-sectional, online survey from December 2021 to February 2022 to investigate GPs’ perception towards NCSP, job satisfaction, and intentions to remain in rural area. Eligible participants were GPs who were required to provide health services as part of NCSP at township health centres of 9 provinces which could represent all NCSP GPs in China. Multinomial logistic regression analyses were performed to explore the associations between policy perceptions, job satisfaction, and intentions to remain.

Results

Of 3615 GPs included in the analysis, 442 (12.2%) would like to remain in rural area and 1266 (35.0%) were unsure. Results of the multinomial logistic regression analyses showed that compared with GPs who would leave, GPs with higher perception scores for the restriction on taking postgraduate exam (RRR: 1.93, 95% CI 1.72, 2.16) and the commitment to work for six years (RRR: 1.53, 95% CI 1.31, 1.78) were more likely to remain. In contrast, GPs who had higher perception scores for completing standardised residency training (RRR: 0.75, 95% CI 0.64, 0.88) and passing National Medical Licensing Examinations (RRR: 0.74, 95% CI 0.62, 0.87) were more likely to leave. GPs who were satisfied with the freedom of choosing work methods (RRR: 1.52, 95% CI 1.25, 1.84) and chances of promotion (RRR: 1.60, 95% CI 1.32, 1.94) were more likely to remain.

Conclusions

This study highlights the significance of policy perception and job satisfaction on GPs’ intentions to remain in rural area. Factors such as career advancement and the empowerment of GPs to build on and use their skills and abilities should be taken into account when designing rural placement programmes.
Banana bunchy top virus movement protein induces resistance in banana against Fusarium wilt
Authors: Wang Weiying , Wan Wenqiang , Chen Qian , Wei Taiyun , Zhang Hongxiang
Published: April 2024

Banana Fusarium wilt, which is known as Panama disease and caused by Fusarium oxysporum f. sp. cubense (Foc), is a destructive disease that can lead to plant death and complete loss of banana plantations. For obtaining resistant varieties against Fusarium wilt, research is required to elucidate the mechanisms of genetic resistance to Foc. In this research, the banana plants infected with banana bunchy top virus (BBTV) showed resistance to Foc. RNA-seq results showed that the infection with Foc upregulated many resistance-related genes in BBTV-positive plants, especially genes related to ROS production. It was also found that more H2O2 in BBTV-positive plants was induced during Foc infection. The movement protein (MP) of BBTV could increase H2O2 levels by promoting the accumulation of MaSGT1a (suppressor of the G-two allele of SKP1) via direct interaction. Thus, the resistance to Foc induced by BBTV may attribute to MP increasing MaSGT1a protein levels to induce ROS production. MaSGT1a could promote the accumulation of R proteins MaRPM1 and MaRPP8, and increase H2O2 levels in Nicotiana benthamiana. The expression of R proteins in Nicotiana benthamiana caused an increase in H2O2 levels and promoted resistance to Foc. Thus, the MP of BBTV could induce plant defense through interaction with MaSGT1a. We speculate that MaSGT1a is likely involved in R gene-mediated resistance and thereby promotes the resistance of banana against Foc.
Adjunctive immunotherapeutic agents in patients with sepsis and septic shock: a multidisciplinary consensus of 23
Authors: Girardis Massimo , Coloretti Irene , Antonelli Massimo , Berlot Giorgio , Busani Stefano , Cortegiani Andrea , De Pascale Gennaro , De Rosa Francesco Giuseppe , De Rosa Silvia , Donadello Katia , Donati Abele , Forfori Francesco , Giannella Maddalena , Grasselli Giacomo , Montrucchio Giorgia , Oliva Alessandra , Pasero Daniela , Piazza Ornella , Romagnoli Stefano , Tascini Carlo , Viaggi Bruno , Tumbarello Mario , Viale Pierluigi
Published: April 2024

Background

In the last decades, several adjunctive treatments have been proposed to reduce mortality in septic shock patients. Unfortunately, mortality due to sepsis and septic shock remains elevated and NO trials evaluating adjunctive therapies were able to demonstrate any clear benefit. In light of the lack of evidence and conflicting results from previous studies, in this multidisciplinary consensus, the authors considered the rational, recent investigations and potential clinical benefits of targeted adjunctive therapies.

Methods

A panel of multidisciplinary experts defined clinical phenotypes, treatments and outcomes of greater interest in the field of adjunctive therapies for sepsis and septic shock. After an extensive systematic literature review, the appropriateness of each treatment for each clinical phenotype was determined using the modified RAND/UCLA appropriateness method.

Results

The consensus identified two distinct clinical phenotypes: patients with overwhelming shock and patients with immune paralysis. Six different adjunctive treatments were considered the most frequently used and promising: (i) corticosteroids, (ii) blood purification, (iii) immunoglobulins, (iv) granulocyte/monocyte colony-stimulating factor and (v) specific immune therapy (i.e. interferon-gamma, IL7 and AntiPD1). Agreement was achieved in 70% of the 25 clinical questions.

Conclusions

Although clinical evidence is lacking, adjunctive therapies are often employed in the treatment of sepsis. To address this gap in knowledge, a panel of national experts has provided a structured consensus on the appropriate use of these treatments in clinical practice.
Diagnostic features of paediatric catatonia: multisite retrospective cohort study
Authors: Smith Joshua R. , York Tasia , Baldwin Isaac , Fuchs Catherine , Fricchione Gregory , Luccarelli James
Published: April 2024

Background

Catatonia is a neuropsychiatric disorder characterised by psychomotor changes that can affect individuals across the lifespan. Although features of catatonia have been described in adults, the most common clinical symptoms among paediatric patients with catatonia are not well characterised.

Aims

The goal of this study was to characterise the symptoms of catatonia demonstrated by paediatric patients, and to explore demographic and diagnostic factors associated with greater catatonia severity.

Method

We conducted a multicentre retrospective cohort study, from 1 January 2018 to 6 January 2023, of patients aged 18 and younger with a clinical diagnosis of catatonia and symptom assessment using the Bush Francis Catatonia Rating Scale (BFCRS).

Results

A total of 143 patients met inclusion criteria. The median age was 15 (interquartile range: 13–16) years and 66 (46.2%) patients were female. Neurodevelopmental disabilities were present in 55 (38.5%) patients. Patients demonstrated a mean of 6.0 ± 2.1 signs of catatonia on the Bush Francis Catatonia Screening Item, with a mean BFCRS score of 15.0 ± 5.9. Among the 23 items of the BFCRS, six were present in >50% of patients (staring, mutism, immobility/stupor, withdrawal, posturing/catalepsy, rigidity), and four were present in <20% of cases (waxy flexibility, mitgehen, gegenhalten, grasp reflex). In an adjusted model, patients with neurodevelopmental disorders demonstrated greater BFCRS severity than those with other diagnoses.

Conclusions

Catatonia was diagnosed in a range of mental health conditions. Further research is needed to define optimal diagnostic criteria for catatonia in paediatric patients, and clarify the clinical course of the disorder.
Endoscopic Retrolabyrinthine Craniotomy for Exposure of the Trigeminal Nerve Root Entry Zone: Volumetric Analysis of Anatomic Exposure in the Cadaver
Authors: Chau Dominic , Olszewski Adam , D'Agostino Anna K. , Ellsperman Susan , Slattery William H. , Lekovic Gregory P.
Published: April 2024

BACKGROUND AND OBJECTIVES:

Exposure of the root entry zone (REZ) of the trigeminal nerve (TN) for microvascular decompression is commonly obtained with a retrosigmoid approach, with or without endoscopic assistance. We hypothesized that adequate exposure of the TN REZ could be obtained through an endoscopic retrolabyrinthine (RL) approach. We aim to quantify exposure of the REZ of the TN using endoscopic RL approach, with and without drilling of the suprameatal tubercle of the internal auditory canal.

METHODS:

Surgical dissection was performed bilaterally on 3 embalmed cadaveric human heads at the anatomy laboratory of the House Institute. Heads were scanned for volumetric analysis using 3D Slicer software both before and after dissection. Extent of exposure was quantified in 2 ways: first, by assessment of the surgeon's ability to visualize 16 predetermined anatomic landmarks with the endoscope and second, we estimated the “working” area by placing fiducials under the fully endoscopic view and calculating the resultant 3D volume.

RESULTS:

Using the standard endoscopic RL approach, an average of 13.8 landmarks (range 12-16) was visualized. The estimated working volume exposed by the RL on each side of each head varied from 189.28 to 527.85 mm3. Drilling of the suprameatal tubercle provided both increases in landmark visualization and, on average, an additional 55 mm3 of working volume.

CONCLUSION:

The endoscopic RL approach is a viable alternative to the standard retrosigmoid approach. Potential advantages of the RL include a more lateral trajectory that minimizes the need for cerebellar retraction and a shorter working distance and shallower angle to the cerebellopontine angle. Potential disadvantages include longer surgery time, increased technical difficulty of exposure, and potential for cerebrospinal fluid leak and or hearing loss.
Multidisciplinary Surgical Approach Using Augmented Reality Preplanning for Resection of Giant Thoracic Schwannoma With Robotic-Assisted Thoracoscopic Mobilization
Authors: Monek Adam C. , Mitha Rida , Andrews Edward , Sarkaria Inderpal S. , Agarwal Nitin , Hamilton D. Kojo
Published: April 2024

BACKGROUND AND IMPORTANCE:

In adults, primary spinal cord tumors account for 5% of all primary tumors of the central nervous system, with schwannomas making up about 74% of all nerve sheath tumors. Thoracic schwannomas can pose a threat to neurovasculature, presenting a significant challenge to safe and complete surgical resection. For patients presenting with complex pathologies including tumors, a dual surgeon approach may be used to optimize patient care and improve outcomes.

CLINICAL PRESENTATION:

A 73-year-old female previously diagnosed with a nerve sheath tumor of the fourth thoracic vertebra presented with significant thoracic pain and a history of falls. Imaging showed that the tumor had doubled in size ranging from T3 to T5. Augmented reality volumetric rendering was used to clarify anatomic relationships of the mass for perioperative evaluation and decision-making. A dual surgeon approach was used for complete resection. First, a ventrolateral left video-assisted thoracoscopic surgery was performed with robotic assistance followed by a posterior tumor resection and thoracic restabilization. The patient did well postoperatively.

CONCLUSION:

Although surgical treatment of large thoracic dumbbell tumors presents a myriad of risks, perioperative evaluation with augmented reality, new robotic surgical techniques, and a dual surgeon approach can be implemented to mitigate these risks.
Urban Public Works in Spatial Equilibrium: Experimental Evidence from Ethiopia
Authors: Franklin Simon , Imbert Clément , Abebe Girum , Mejia-Mantilla Carolina
Published: April 2024

This paper evaluates a large urban public works program randomly rolled out across neighborhoods of Addis Ababa, Ethiopia. We find the program increased public employment and reduced private labor supply among beneficiaries and improved local amenities in treated locations. We then combine a spatial equilibrium model and unique commuting data to estimate the spillover effects of the program on private sector wages across neighborhoods: under full program rollout, wages increased by 18.6 percent. Using our model, we show that welfare gains to the poor are six times larger when we include the indirect effects on private wages and local amenities. (JEL H76, I38, J22, J31, O15, O18, R23)
Resisting Social Pressure in the Household Using Mobile Money: Experimental Evidence on Microenterprise Investment in Uganda
Authors: Riley Emma
Published: April 2024

I examine whether changing the form of disbursement of a microfinance loan enables female microfinance borrowers to overcome intra-household sharing pressure and grow their businesses. Using a field experiment with 3,000 borrowers in Uganda, I compare the disbursement of a loan as cash to disbursement onto a digital account. After 8 months, women who received their microfinance loan on the digital account had 11 percent higher (US$70) business capital and 15 percent higher (US$18) profits compared to those who received their loan as cash. Impacts were greatest for women who experienced pressure to share money with others in the household at baseline. (JEL C93, D13, G51, J16, O12, O16)
CircSeqAlignTk: An R package for end-to-end analysis of RNA-seq data for circular genomes
Authors: Sun Jianqiang , Fu Xi , Cao Wei
Published: April 2024

RNA sequencing (RNA-seq) technology has become one of the standard tools for studying biological mechanisms at the transcriptome level. Advances in RNA-seq technology have led to the development of numerous publicly available tools for RNA-seq data analysis. Most of these tools target linear genome sequences despite the necessity of studying organisms with circular genome sequences. For example, studying the infection mechanisms of viroids which comprise 246–401 nucleotides circular RNAs and target plants may prevent tremendous economic and agricultural damage. Unfortunately, using the available tools to construct workflows for the analysis of circular genome sequences is difficult, especially for non-bioinformaticians. To overcome this limitation, we present CircSeqAlignTk, an easy-to-use and richly documented R package. CircSeqAlignTk offers both command line and graphical user interfaces for end-to-end RNA-seq data analysis, spanning alignment to the visualisation of circular genome sequences, via a series of functions. Moreover, it includes a feature to generate synthetic sequencing data that mirrors real RNA-seq data from biological experiments. CircSeqAlignTk not only provides an easy-to-use analysis interface for novice users but also allows developers to evaluate the performance of alignment tools and new workflows.
Evaluation of the administrative management model in a municipality in Peru, incorporating the Intelligent Organization Theory
Authors: Tarrillo Carrasco José Elider , Becerra Santa Cruz José Humberto , Luna Rioja Carlos Hugo , Saldaña Castillo José Carloman , Morán Santamaría Rogger Orlando , Cúneo Fernández Francisco Eduardo , Lizana Guevara Nikolays Pedro , Coronel Benites Cynthia Vanessa
Published: April 2024

Background This study begins with the analysis of the current management models and their degree of effectiveness in municipal administration. Its aim is to design an administrative management model that enables effective administration in the District Municipality of Nueva Cajamarca, Peru, based on the theory of intelligent organizations. Method The research type employed in this study is diagnostic-propositional, utilizing both deductive and inductive methods, in alignment with a mixed-method approach and a non-experimental nature of the study. Data was collected from three distinct populations, including the 189 municipal employees engaged in administrative roles, who were subjected to a 50-question survey. This survey aimed to assess their perceptions regarding the current management model and its relationship with administrative effectiveness. Additionally, interviews were conducted with three experts to gain deeper insights into the behavior of the variables under investigation. Results Finally, documentary information about the management models currently in use was collected. This facilitated the triangulation of data collection, processing, analysis, and inferences from three sources of information. The results reveal a positive, direct, and significant correlation between the management model and administrative effectiveness. It becomes evident that the current management model is deficient, resulting in a low level of administrative effectiveness. Conclusion The management model based on the theory of intelligent organizations was validated using a rubric by experts in effective management. The main pillars of this model include transformational leadership, structural change, and cultural change.
Automatic Vehicle Fueling System using PLC Controlled Robotic Arm - A Simulation Design
Authors: Venugopal Chitra , Thangavel Bhuvaneswari
Published: April 2024

The objective of this research is to simulate an automatic fuelling system using a PLC LogixPro simulation. The system includes the “FASS” concept, which is Fast, Accurate, Safe and Simple, to allow car users to have an efficient fuel filling system. The design concept consists of three processes – identification of the vehicle, payment, and filling with the fuel. The first process identifies the presence of the car by the in-floor weight sensors. The weight sensor identifies the car, locks it in position, and activates the payment system. The second process activates the payment system. After payment is completed, the fuel cap will be opened to enable the system to start filling the fuel. If the payment doesn’t go through the car will be released, manual operation will be initialized, and the entire system will be reset. A timer is included in the payment section to process the payment. In the third process, the filling arm is extended to the car, the fuel cap is opened, the fuel pump is inserted into the tank, and fuel is directed into the tank. Once the tank is full, filling is stopped, the pump is ejected, the fuel cap is closed, and the arm returned back to its position. Thus, an automatic vehicle fuelling system is created to overcome the problems of poor safety and longer waiting time during peak hours. The fuel cap is activated and deactivated by pressure and the sensor filler is stopped by a level sensor. The pump insert is activated and deactivated by a photosensor.
LGBTQ+ individuals are not explicitly represented in emergency medicine simulation curricula
Authors: Bod Jessica , Buck Samuel , Chandler Iris , Goldflam Katja , Tsyrulnik Alina , Coughlin Ryan , Fujimoto Jessica , Joseph Melissa , Della-Giustina David , Phadke Manali , Boatright Dowin
Published: April 2024

Background Medical educational societies have emphasized the inclusion of marginalized populations, including the lesbian, gay, bisexual, transgender and queer (LGBTQ+) population, in educational curricula. Lack of inclusion can contribute to health inequality and mistreatment due to unconscious bias. Little didactic time is spent on the care of LGBTQ+ individuals in emergency medicine (EM) curricula. Simulation based medical education can be a helpful pedagogy in teaching cross-cultural care and communication skills. In this study, we sought to determine the representation of the LGBTQ+ population in EM simulation curricula. We also sought to determine if representations of the LGBTQ+ population depicted stigmatized behavior. Methods We reviewed 971 scenarios from six simulation case banks for LGBTQ+ representation. Frequency distributions were determined for major demographic variables. Chi-Squared or Fisher’s Exact Test, depending on the cell counts, were used to determine if relationships existed between LGBTQ+ representation and bank type, author type, and stigmatized behavior. Results Of the 971 scenarios reviewed, eight (0.82%) scenarios explicitly represented LGBTQ+ patients, 319 (32.85%) represented heterosexual patients, and the remaining 644 (66.32%) did not specify these patient characteristics. All cases representing LGBTQ+ patients were found in institutional case banks. Three of the eight cases depicted stigmatized behavior. Conclusions LGBTQ+ individuals are not typically explicitly represented in EM simulation curricula. LGBTQ+ individuals should be more explicitly represented to reduce stigma, allow EM trainees to practice using gender affirming language, address health conditions affecting the LGBTQ+ population, and address possible bias when treating LGBTQ+ patients.
Abdominal ultrasonography as a tool to quantify adipose tissue in critically ill patients
Authors: York Jesse , Remmert Nicole , Nugent Kenneth
Published: April 2024

Adipose tissue has important roles in both healthy and disease states. Quantifying adipose tissue composition and distribution may help predict outcomes and guide management in intensive care unit patients. Computed tomography is the preferred method for measuring adipose tissue but has drawbacks, such as radiation exposure and the need to transport patients. Ultrasonography is a safer, more convenient alternative for bedside assessment of body fat composition. This review examines the relevance of adipose tissue in critical illness and describes ultrasound techniques to quantify subcutaneous, visceral, and epicardial adipose tissue depots. Standardized protocols to measure subcutaneous fat thickness at multiple sites and approaches to estimate visceral fat using the supraumbilical or xipho-umbilical lines are discussed. A technique to quantify epicardial fat thickness using transthoracic echocardiography is also presented. While more research is needed, bedside ultrasonography shows promise for monitoring adipose tissue changes and their potential associations with outcomes in critically ill patients.
 
Key words: Ultrasonography, critical illness, body composition, subcutaneous adipose tissue, visceral adipose tissue, epicardial adipose tissue
Los “detenidos-desaparecidos” en Colombia y México Aportes para una historia comparada de la desaparición forzada desde las organizaciones de familiares (1968-1988)
Authors: Espinosa Moreno Fernanda
Published: April 2024

Este texto se propone aportar a una historia comparada de la desaparición forzada desde las organizaciones de familiares en Colombia y en México, en el periodo denominado como “guerra sucia” en ambos países, entre 1968-1988. A lo largo del texto reflexionamos sobre el concepto de “detenido-desaparecido” consolidado y propuesto desde las organizaciones de familiares.
Bargaining with Voluntary Disclosure and Endogenous Matching
Authors: Davis Andrew M. , Hyndman Kyle
Published: April 2024

We investigate a bargaining setting between an “informed” player, who has private information, and an “uninformed” player. The informed player has the option to truthfully disclose private information in two unique environments. In the first environment, the informed player is randomly matched with an uninformed player and, after matching, can voluntarily disclose private information prior to a negotiation taking place. In the second environment, the informed player can voluntarily disclose private information before any endogenous matching between players (and subsequent negotiation) takes place. We begin by examining these environments theoretically. When disclosure occurs after matching, we show that low informed types should disclose more often than high informed types to avoid disagreement. However, when disclosure occurs before matching, for a range of parameter values, we show that high informed types should disclose more than low informed types to secure a better match. We test these predictions in a controlled human-subjects experiment and verify many of the theoretical predictions. Among other results, we find that low informed types do indeed disclose their private information to avoid disagreement and that, when it is in their interest to do so, high informed types disclose their private information to secure a better match. Another key insight is that high uninformed types benefit from disclosure regardless of whether it occurs before or after matching.

This paper was accepted by Axel Ockenfels, behavioral economics and decision analysis.

Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2022.03566 .
Amino Acids Oral Treatment for the Amelioration of Skin, Hair, and Nails Conditions: An Open-Label Study
Authors: Nobile Vincenzo , Cestone Enza , Pisati Marta , Roveda Gloria
Published: April 2024

This study aimed at investigating the effect of a food supplement in ameliorating the skin, hair, and nails conditions. An open-label study on 21 women with mild-to-moderate aging all over the face (including wrinkles/fine lines, loss of skin firmness/elasticity, and normal to dry skin), acute telogen effluvium, and brittle/fragile nails was conducted. Skin (moisturization, elasticity, profilometry, and skin thickness), hair (pull test and phototricogram), and nails (clinical analysis of nail plate status) parameters were measured at baseline, and after 28 and 84 days of intake. The clinical analysis carried out by a board-certified dermatologist and the self-evaluation integrated the instrumental measurements. After 28 (D28) and 84 (D84) days of product intake the skin moisturization (superficial moisturization: +6.0% at D28 and +9.2% at D84; deep moisturization: +4.2% at D28 and +7.6 at D84), elasticity (R0 parameter: -11.5% at D28 and -19.3% at D84; R2 parameter: +7.8% at D28 and +11.9 at D84), profilometry (wrinkle depth: -9.5% at D28 and -14.5% at D84; skin smoothness: -4.9% at D28 and -7.7% at D84) and thickness (+0.11 mm at D28 and +0.24 mm at D84) were improved. At D84 the density of telogen hair was reduced by 9.5%; while at day 28 pull test was not indicative for telogen effluvium. The nail status was improved in most of the enrolled subjects. The instrumental measured effects were perceived by both the dermatologist and the enrolled subjects. Our results highlight the test product efficacy in improving the skin, hair, and nails conditions.
Modifiable and Non-Modifiable Factors Associated with Gastric Cancer
Authors: Al-Naimi Noora , Aljumaily Maryam , Al-Amer Rawdhah , Hamdan Aya , Tayyem Reema
Published: April 2024

Numerous studies have investigated the associations between dietary components, behavioral patterns, and susceptibility to gastric cancer (GC). Diet and lifestyle cover a spectrum of both protective and harmful factors associated with GC. Additionally, non-modifiable factors such as age, gender, blood type, family history, and genetic predispositions may influence GC development. This review aims to explore the interplay between modifiable and non-modifiable factors, along with dietary habits and lifestyle practices, in relation to GC risk and the potential underlying mechanisms. We have synthesized the primary findings of observational studies (case-control and cohort), systematic reviews, and meta-analyses pertaining to preventive and deleterious factors affecting the incidence of gastric cancer. A literature search was conducted on Google Scholar, MEDLINE (PubMed), ScienceDirect, and Scopus for articles published in English from 2001 to 2024. The main search terms included body weight and body fat; diet; fruits and vegetables; meats and processed meats; fried and fast foods; milk and dairy products; salty foods; food and dietary patterns; fat and sweets intake; alcohol consumption; smoking; physical activity; age; sex; family history; blood type; genetics; and medication, and the risk of gastric cancer. Unhealthy dietary patterns, consumption of fried and fast foods, salty foods, alcohol, and smoking have been associated with an increased risk of GC. Non-modifiable factors such as advanced age, male sex, family history, genetics, and blood type A were linked to an elevated risk of GC. Conversely, physical activity and high consumption of fresh fruits and vegetables may prevent GC occurrence due to the presence of antioxidants, fiber, and polyphenols. While many studies have demonstrated that dietary patterns loaded with red and processed meats were associated with a high risk of GC, others have yielded inconclusive results. Controversial findings regarding the relationship between body weight and body fat, medications, milk and dairy products, and fat and sweets consumption with the risk of GC were also observed. Adequate diet modification and addressing preventable factors may play a pivotal role in reducing the incidence of gastric cancer.
Sensory Acceptance, Microbiological, and Nutritional Properties of a Sausage-like Meat Product Produced with Partial Inclusion of Rhynchophorus palmarum Larvae
Authors: El-Salous Ahmed , Moreno Luis Zuniga- , Melendez Jesús R. , Castro Evelyn , Espinoza Winston , Castillo Daniel Mancero- , Rodríguez Pablo Nuñez- , Arcos Freddy
Published: April 2024

Rhynchoporus palmarum is widely considered to be a harmful pest of many crops. Nevertheless, it is highly valued as a food source in some countries, providing valuable components, mainly protein and fatty acids. The aim of this study was to develop a sausage with partial incorporation of R. palmarum larvae and to explore its potential as an alternative ingredient, taking into account the sensory acceptance of the final product, as well as nutritional and microbiological aspects. Experimental formulations containing 30, 20, and 10% larvae (F1, F2, F3, respectively) partially replaced pork meat and vegetable fat, and a control (F0) without R. palmarum larvae. One hundred and fifty semi-trained panelists rated the taste, aroma, color, texture, and overall acceptability of the four formulations using a 5-point hedonic scale. F2 showed favorable sensory acceptance for taste, color, texture, and overall acceptability, with no significant statistical difference from F0, but aroma showed a significantly better score than F0. Nutritional data showed increasing patterns for energy, fat, and sodium, while decreasing values for cholesterol, protein, and total carbohydrate for F1, F2, and F3, respectively. The microbiological characteristics of the experimental formulations for aerobic plate count, E. coli count, S. aureus, and Salmonella spp. met the requirements of the Ecuadorian standard NTE 1338:2012 for cooked meat products. This research highlights the potential of Rhynchophorus palmarum larvae as a viable and sustainable option for partial meat substitution in sausage production, offering an innovative approach to improve nutritional value and diversify product offerings in the food industry.
Ancient Indian Diet – A Balanced Diet for the Healthy Diversity of Gut Microbiota and Management of Asthma
Authors: Das Monalisa , Thajuddin Nooruddin , Patra Sanjib , Pundir Megha
Published: April 2024

Modernization, a stressful lifestyle, attachment to a Western diet, and the use of preserved and processed foods lead to a loss of homeostasis of intestinal microbial diversity. The use of refined flour and refined sugar, which lack of micro biota-accessible carbohydrates (MAC), means dietary fiber high salt consumption, and saturated fats in fried and packaged foods cause gut microbiota dysbiosis. Microbial dysbiosis caused by high fat, salt, and lack of fiber causes several metabolic diseases, including asthma. The objective of the study is to develop a hypothetic model that “Ancient Indian diet” which is inspired by thousands of years of Indian science, including Yoga, Ayurveda, and Naturopathy may maintain the diversity of the gut microbiota and may inhibits the asthmatic symptoms by enhancing the growth of antiasthmatic bacteria. A keyword search utilizing the phrases "western diet, beneficial microbiota, asthma, gut microbial dysbiosis, Ancient Indian diet" was conducted electronically search through the SCOPUS ,Science Direct ,PubMed, Web of Science, and PsycINFO databases. Search criteria are divided into two arms the first one includes different full-text research papers including how Indian diet altered gut microbial composition. The second one, we included the full text including how presence of certain gut microbiota can prevents the asthmatic attack. To find publications that met the eligibility requirements, the first and third authors separately reviewed each publication's title and abstract using the previously described inclusion criteria. We have included 124 research articles, which is published between 1989 to 2024. A plant-based ancient Indian diet increases the diversity of Bacteroidetes, Actinobacteria, Prevotella, Bifidobacterium, Lactobacillus, Ruminococcus, Roseburia, Lactobacillus, Lachnospira, Akkermansia and shows inhibitory effects against Clostridium, E. coli, Staphylococcus, Haemophilus, Moraxella and Neisseria. The ancient Indian diet a low-fat lacto-vegetarian diet maintains gut microbiota homeostasis, and suppresses the growth of pathogenic asthmatic bacteria and promotes the growth of beneficial asthmatic bacteria.
BRASIL E CANADÁ:
Authors: Danelon Maria Cristina Tavares de Moraes , Luciana Cioffi de Barros Patrícia
Published: April 2024

O presente artigo é fruto de experiências vivenciadas por duas autoras. Ambas atuam na área de inclusão de uma Secretaria Municipal de Educação no interior do estado do Rio de Janeiro- Brasil. Uma delas teve a oportunidade de participar de um programa financiado pela CAPES, no Canadá, cuja premissa era a apresentação de projetos de intervenção pedagógica com uma proposta de trabalho a ser desenvolvida na escola ou na rede de ensino de atuação. A outra está em estágio de pós doutoramento no Programa de Pós-Graduação em Educação Cultura e Comunicação (UERJ). Ambas estão participando de ações do Núcleo de Educação Especial e Inclusiva (NEEI- UERJ). O estudo, em andamento, justifica-se tendo em vista que as práticas, que buscam ser inclusivas, desenvolvidas até o presente momento, na rede de ensino pública municipal, ainda tem encontrado muitas barreiras. O objetivo geral é obter dados primários acerca das ações inclusivas fora do Brasil onde há outros referenciais para confrontar as realidades experenciadas no município onde as autoras atuam. Nesse sentido, uma análise comparativa favorecerá um olhar ampliado sobre a realidade atual da inclusão no município e possibilitará a projeção de políticas públicas mais inclusivas. Espera - se encontrar resultados consistentes que possam vislumbrar melhores práticas para que as pessoas com deficiência tenham, de forma eficaz, garantido o seu direito constitucional à Educação.
Robotic surgery in low- and middle-income countries
Authors: Burke J , Gnanaraj J , Dhanda J , Martins B , Vinck EE , Saklani A , Harji D
Published: April 2024

How is robotic-assisted surgery being developed and implemented in low- and middle-income countries, and what challenges do we face?
The ups and downs of down under
Authors: Fleming Simon
Published: April 2024

An insight into fellowship pathways for surgical trainees in Australia and how they compare to the UK.
Speckle visual cryptography for credential authentication
Authors: Jiao Anqing , Zhu Yupeng , Mi Zhaoke , Zhou Xinlong , Zhu Qiaofen , Ke Changjun , Shi Yishi
Published: April 2024

Based on the high random distribution characteristic of the natural speckle image, a new method of speckle visual cryptography, to the best of our knowledge, is designed by combining the natural speckle image with the secret key in visual cryptography. Specifically, we designed an authentication system for user credentials by combining speckle visual cryptography and the QR code. By using the speckle visual cryptography method, the image of the QR code carrying user authentication information is hidden in the speckle image, and the speckle image is printed on the paper credentials. Through a simulation and analog experiments, we verify the possibility of applying speckle visual cryptography to a user credentials authentication system, compare the improved grayscale reordering algorithm and grayscale reordering algorithm, and prove that the improved grayscale reordering algorithm has more advantages in this system by comparing the PSNR and SSIM. Finally, the y-interference ability and the uniqueness of the virtual secret key in the system are analyzed to prove that the secret key has high anti-interference ability and security.