bluuebunny/crossref_metadata_2025 huggingface dataset:
PANCREATIC CARCINOMA IN INFANCY — AN ELECTRON MICROSCOPIC STUDY —
Authors: Kakudo Kennichi , Sakurai Masami , Miyaji Toru , Ikeda Yoshikazu , Satani Minoru , Manabe Hisao
Published: December 2008

A case of a malignant epithelial tumor of tail of the pancreas in a 3‐year‐old Japanese girl is presented. This is the fourth case reported with ultrastructural study. This type of pancreatic carcinoma of the infant has no endocrine granules or endocrine function. The ultrastructural study suggests that the tumor is derived from a primitive epithelial cell with differentiation toward acinar cell.
FAMILIAL NEPHROPATHY ASSOCIATED WITH HEPATIC TYPE OF GLYCOGEN STORAGE DISEASE
Authors: Sonobe Hiroshi , Ogawa Katsuo , Takahashi Isao
Published: December 2008

The female patient was diagnosed as having Von Gierke's disease at 14 years of age, based on clinical manifestations, laboratory examination and liver biopsy. At 19 years of age she had uremia and died from its deterioration at 24 years of age. The parents were consanguineous, and a 27‐year‐old sister is presently hospitalized for renal Insufficiency with hepatomegaly. On autopsy, the patient's kidneys were highly contracted and contained a number of small cysts, mainly in the medulla. Histological examination indicated periglomerular fibrosis, glomerular hyalinization, tubular atrophy or cystic dilatation and intersitial fibrosis with round cell infiltration. These findings correspond to Fanconi's familial juvenile nephronophthlsis, except for age. The liver was markedly enlarged and Indicated severe glycogen deposits, but the kidney did not contain glycogen deposits. It can, therefore, be presumed that the renal lesions were not a secondary consequence of long‐term glycogen deposits but that renal and hepatic lesions were associated with each other.
SANFILIPPO B SYNDROME — A CASE REPORT —
Authors: Shimamura Kayako , Hakozaki Hando , Takahashi Kiyoshi , Kimura Atsushi , Fujino Junko , Suzuki Yoshiyuki , Nakamura Norimasa
Published: December 2008

An autopsy case of a 9 years and 5 months old gargoyle girl diagnosed as Sanfilippo B syndrome by the biochemical demonstration of a large amount of heparan sulfate in urine and some organs and of deficiency of α‐N‐acetyl‐D‐glucosaminidase in the liver and brain was reported. The morphological changes characterized by cytoplasmic swelling and vacuolization were more generalized than those which had been described in previously reported cases. Histochemically, accumulation of variable amounts of acidic glycosaminoglycans and compound lipids, presumably gangliosides and phospholipids, was substantiated in the vacuolated cells of various visceral organs and in the ballooned neuronal cells. Ultrastructurally, numerous inclusions found in these cells were largely divided Into two types; flocculent reticulogranular and osmiophilic, mostly laminated materials, many of which were bound by a single unit membrane. Enzyme cytochemistry proved acid phosphatase activity in the majority of the inclusions in fibroblasts and nbrocytes biopsied from the skin. Rough endoplasmic reticulum in these cells was markedly dilated with reticulogranular materials. The morphological changes of the present case and their pathogenesis were discussed.
GYTOLOGICAL CHARACTERIZATION AND HISTOGENESIS OF EWING'S SARCOMA
Authors: Takahashi Kiyoshi , Sato Tadashi , Kojima Mizu
Published: December 2008

In addition to a light‐microscopical and histochemical investigation of primary and metastatic lesions in 27 cases of Ewing's sarcoma, biopsy materials from the primary bone lesions of 7 patients with this neoplasm were examined histochemically, enzyme ‐cytochemically and electron microscopically to elucidate the histogenesis and nature of the neoplasm. Ultrastructural observation has revealed that besides intracytoplasmic and extracellular deposition of glycogen the tumor cells possess several cytological features characterized by intracytoplasmic microfilaments of varying thickness up to 80 Å, occasional appearance of dense patches, fat droplets, desmosomal connections and reminiscent attachment bodies. The tumor cells are mostly round, oval or polygonal in shape, but spindle or elongated cells are intermingled and occasionally contain well‐developed rough endoplasmic reticulum, resembling pericytes or fibroblasts. In the intercellular spaces amongst the tumor cells, varying amounts of variable‐shaped amorphous materials are found, which are compatible with acid mucopolysaccharides and glycoproteins histochemically verified. These findings may suggest that Ewing's sarcoma is a highly malignant neoplasm originating from a transitional cell developed from pericytes to vascular smooth msucle cells in the bone marrow.
TWO AUTOPSY CASES OF PRIMARY ADENOACANTHOMA OF THE STOMACH
Authors: Taira Yoshitsugi , Ogata Hirohumi , Tsuchiyama Hideo
Published: December 2008

The incidence of primay adenoacanthoma of the stomach is extremely low. In the Sasebo City Hospital, there were only two autopsy cases of adenoacanthoma among 46 cases of gastric cancer during the past four years. The first case was a 45 year‐old male with adenoacanthoma. The second case was a49 year‐old female with metachronous triple cancers; adenoacanthoma of the stomach, squamous cell carcinoma of the uterus and squamous cell carcinoma of the tongue. Histological examination showed muconodular adenocarcinoma of the stomach at the time of first operation and adenoacanthoma at the time of autopsy. These two cases suggest that adenoacanthomas of the stomach may originate from squamous metaplasia or squamous differentiation of a preexisting glandular carcinoma.
CONGENITAL BRAIN AND FACIAL ANOMALIES IN THE D1 TRISOMY SYNDROME—REPORT OF A CASE AND A REVIEW OF LITERATURE—
Authors: Miyasaki Kichihei , Murao* Shinichi , Nakamura** Kazushige , Hashimoto*** Yoshiho
Published: December 2008

On 58 cases of D1 trisomy, which were classified as those with seven separate D chromosomes, including the one in this report, congenital brain and facial anomalies were reviewed and discussed. Congenital brain defects in this syndrome might be classified into three groups: (I) grossly normal brain, (II) absence of olfactory bulbi and tracts, but normally‐separated cerebral hemisphere, and (III) completely or {incompletely uncleaved hemispheres with absence of olfactory bulbi and tracts. 25.9% of the cases were found in group I, 56.9% in group II, and 17.2% in group III, respectively. Although facial anomalies predicted the brain defects in group III, malformations of the face were not concerned with the brain defects in group I and II. There were no cases with both, normal brain and face.
TUMOR GROWTH OF THE RETICULOENDOTHELIAL SYSTEM
Authors: Kojima Mizu
Published: December 2008

From the author's hitherto studies on the reticuloendothelial system (RES) it was concluded that the RES is not a single cell system of identical origin, morphology and function but is a group of several types of cells of different origin. From this point of view the heterogeneity of tumors of the RES was studied to reveal the following results. Tumors of the “Reticulo‐endothel” (Aschoff) reveal pictures of endothelioma, while tumors of histiocytes in connective tissue show findings of fibrohistiocytoma. Histiocytes and reticulum cells of lymph nodes are respectively independent cells, and reticulum cells do not partake in the development of histiocytosis or histiocytic sarcoma. Follicular lymphoma is a neoplastic growth of reticulum cells having desmosomes in lymph follicles, and tumor cells of the majority of reticulum cell sarcoma are similar to the cells forming the lymph node anlage in the early fetal stage (lymphoreticular cell). Ewing's sarcoma is considered to be a kind of angiopericytoma.
MALIGNANT FIBROUS HISTIOCYTOMA OF BONE
Authors: Yumoto Tokichi , Mori Yoshihiro , Inada Osamu , Tanaka Toshio
Published: December 2008

Existence of an entity called malignant fibrous histiocytoma of bone was emphasized, based on two of our own cases experienced recently and on previous reports related to this tumor. Histologically the tumor resembles markedly the malignant fibrous histiocytoma originating in the soft part. In addition, an attempt was made to clarify its biological behavior, histological subclassification and criteria to be malignant and differential diagnoses from several other bone diseases which sometimes mimic this malignant bone tumor.
ACCELERATED SERUM SICKNESS IN THE RABBIT III. HISTOPATHOLOGICAL STUDY OF THE DEVELOPMENT OF TISSUE INJURIES OF THE HEART
Authors: Shigematsu Hidekazu , Sano Motoaki , Sinjuro Tohru , Kobayashi* Yutaka
Published: December 2008

Histological analysis was performed on the lesions of the coronary artery and myocardium in accelerated serum sickness of the rabbit. Angiitis was seen to occur on the arterioles in the myocardium and was characterized by fibrinoid necrosis of the media with monocyte accumulation. Myocarditis was observed to be rather prominent in the right ventricle, where monocyte accumulation was associated with scattered degeneration or necrosis of myofibrils. In both lesions, the localization of antigen could hardly be detected in the inflammatory foci, suggesting the irrelevancy between the development of the tissue damages and the permanent deposition of immune complexes. Monocytes were seen to participate in the repair of the tissue injury.
MORPHOLOGY OF THE WARTHIN‐FINKELDEY GIANT CELLS IN MONKEYS WITH EXPERIMENTALLY INDUCED MEASLES
Authors: Tajima Masanori , Kudow Satoru
Published: December 2008

The lymphoid tissues of 9 monkeys infected experimentally with wild type measles virus were examined by light and electron microscopy. Multinucleated giant cells of the Warthin‐Finkeldey type were found 7 to 11 days after virus inoculation. The giant cells occurred mostly in the germinal center of lymphatic follicles, where they underwent degeneration and disappeared rapidly. Lymphoid and reticular types of giant cells were recognized. The ultrastructural evidence suggested that some of the nuclei contained in giant cells were formed by an aberrant nuclear cleavage. The majority of giant cells, however, were postulated to arise from virus‐mediated cell fusion, although direct evidence of cell fusion was not seen. Peculiar nuclear changes, in which some nuclei and fragments of the outer nuclear membrane were contained in a common outer membrane of the nuclear envelope, were observed in all lymphoid giant cells. Both cytoplasmic and intranuclear inclusions were seen to be composed of viral nucleoprotein strands. The former were detected in all giant cells of both types, and the latter in occasional nuclei, providing the direct evidence that giant cell formation resulted from replication of the virus.
SYNAPTIC RIBBON IN THE HUMAN PINEALOCYTE
Authors: Kurumado Katsumaro , Mori Wataru
Published: December 2008

An electron microscopic study of a pineal gland which had been obtained from, a 3‐year‐old girl at autopsy revealed the presence of synaptic ribbon in it. Namely, in some parenchymal cells observed, synaptic ribbons, each of which was ca. 700 nm in length and with synaptic vesicles of up to 60 nm in diameter gathering on the surface, were found in the cytoplasmic area adjacent to the cell membrane. The substructure of the synaptic ribbon, i.e., parallel striae running inside along the longitudinal direction, was definitely observed. There is a hypothesis which has been almost accepted that the mammalian pinealocyte is homologous to the photoreceptor cell of the pineal organ in lower animals, and one of the morphological evidences which supports this speculation is the presence of sensory cell elements in both. Since, this kind of work has been hardly done with human materials, the above finding may offer a meaningful information which discloses a part of the real function of the pineal gland in man.
CEROID‐LIKE HISTIOCYTIC GRANULOMA OF GALL‐BLADDER — A PREVIOUSLY UNDESCRIBED LESION —
Authors: Takahashi Kiyoshi , Oka Kuniyuki , Hakozaki Hando , Kojima Mizu
Published: December 2008

In the present study, 13 cases of a peculiar gall‐bladder granuloma characterized by marked proliferation of ceroid‐fllled brown histiocytes were pathomorphologically, histochemically and ultrastructurally examined to define the pathologic features of such a lesion previously undescribed in the literature. The lesion grossly showed a granulomatous appearance of yellow brown to dark brown color developing in the wall of gall‐bladder. Histologically, there was proliferation of histiocytes containing abundant brown pigment granules In their cytoplasm. The pigment granules proved to have staining characteristics closely resembling those of lipogenic ceroid‐like pigment. Ultrastructurally, these granules showed membrane‐bound, pleomorphic osmiophilic inclusions of heterogenous materials. With regard to the pathogenesis of this granuloma, it may be suggested that lipid components of bile juice, particularly unsaturated fatty acids and phospholipids, play an Important role as a source of ceroidogenesis In the proliferating histiocytes.
A HISTOPATHOLOGICAL STUDY ON DIABETIC NEPHROPATHY — LIGHT AND ELECTRON MICROSCOPIC OBSERVATIONS
Authors: Yajima Gompachi
Published: December 2008

A historical review and the present author's study on diabetic nephropathy have been presented.

Three characteristic lesions in KW‐lesion, namely nodular, exudative and doughnut lesion may all be regarded as to be caused by severe circulatory disturbance occurring in intraglomerular branches of the afferent artery. This circulatory disturbance is considered to be brought about by sudden spasm of the peripheral portion of the afferent branches. The diffuse form of Bell may be regarded as to be a slighter type without severe mesangiolysis. Arteriolar hyalinosis and arteriosclerosis and/or atherosclerosis may be caused by intraglomerular circulatory disturbances. The most important problem in diabetic nephropathy, including KW‐lesion and Bell's diffuse form, is to elucidate the mechanism leading to the occurrence of spasm of the peripheral portion of intraglomerular afferent branches. Becker4 found fixation of antiactomyosin‐antibody in the mesangial tissue by the immunofluorescent method. Many studies along this line have been made, but none are adequate in fully explaining the mechanism involved.
INHIBITION OF SENILE AMYLOIDOSIS OF MICE BY BIS‐CARBOXYETHYL GERMANIUM SESQUIOXIDE
Authors: Kuga Norito , Oboshi Shoichi , Sato Hiroshi , Sato Ryuichi
Published: December 2008

A mouse strain, ICR/SLC, was involved In spontaneous amyloidosis with high incidence. The amyloid deposition in this strain was seen mainly in the mucosal propria of duodenum and terminal ileum, liver, spleen, adrenal cortices, and renal glomeruli.

The mice, orally administered more than 300 mg/kg of organic germanium for 22 months since 5 weeks old, did not develop amyloidosis. Half of the mice, given 30 mg/kg of organic germanium for 22 months developed amyloidosis. The mice given 5% carboxymethylcellulose, the solvent of organic germanium, were affected with systemic amyloidosis with high frequency. The results showed that the organic germanium successfully inhibited the occurrence of senile amyloidosis with dose response. The agent did not have any apparent relation to the incidence of hepatic cell carcinoma or pulmonary adenoma which is frequently combined with aged mice. Although the actual mechanism involved is not clear, the evidence of the inhibition of senile amyloidosis by organic germanium may give a light to elucidate the pathogenesis of amyloidosis.
AN AUTOPSY CASE OF H.C.G. SECRETING INTRACRANIAL TERATOMA
Authors: Hirano Torn , Kawai Kioko , Tsuchiyama Hideo
Published: December 2008

An autopsy case of Intracranial malignant teratoma, In an 8 years old female, is presented in this reprot. Clinically, pregnancy test was positive and human chorionic gonadotropins of high titer were found in the urine but precocious puberty was not seen. The tumor was composed of differentiated teratomatous components and embryonal carcinoma with small foci of choriocarcinoma. Embryonal carcinoma seemed to undergo trophoblastic differentiation. These histological findings have been discussed from the viewpoint of its histogenesis and differentiation.
ULTRASTRUCTURE OF THE GIANT CELL IN DE QUERVAIN'S SUBACUTE THYROIDITIS
Authors: Satoh Masanobu
Published: December 2008

The author made an electron microscopic observation on multinucleated giant cells which appear in the thyroid gland in subacute thyroiditis (de Quervain). Numerous typical desmosomes, which intermingled with myriad of mitochondria and small electron dense granules, were found in the cytoplasm of giant cell. Desmosome is one peculiar to the intercellular junction of follicular epithelium of the thyroid gland, and the fact that many desmosomes were found in the cytoplasm is one of the proofs showing this giant cell to be possibly derived from the follicular epithelium.
AN ULTRASTRUCTURAL STUDY ON ANTIBODY PRODUCTION OF THE LYMPH NODES OF RATS WITH SPECIAL REFERENCE TO THE ROLE OF GERMINAL CENTERS
Authors: Terashima Kazuo , Imai Yutaka , Kasajima Takeshi , Tsunoda Kikiya , Takahashi Kiyoshi , Kojima Mizu
Published: December 2008

Antibody‐containing cells in lymph nodes, especially in germinal centers, and in thoracic duct of rats sensitized by Injection of horseradish peroxidase were studied with the immunoperoxidase method. In the primary response, the large germinal center cells began to produce antibody on the 9th day and predominated thereafter In germinal centers, added by intercellular antibody deposition in its late stage. The “ordinary” medium‐sized germinal center cells were a minor component. In the secondary response, the intracellular antibody positivity disappeared immediately after the antigenic rechallenge but reappeared from 6 hours later In the large germinal center cells which further transformed from 48 to 72 hours into the “specifically differentiated” medium‐sized germinal center cells that disappeared by 96 hours after the secondary injection to enter the thoracic duct. Antibody‐containing cells outside the germinal centers appeared 5 to 6 days after the primary injection and were mainly comprised of plasmocytic cell series which was classified Into lnterfollicular large cells, proplasmocytes and plasmocytes. Some results conflicting with the cytologlcal identity of both cell series were presented.
ESTABLISHMENT and CHARACTERISTICS OF A HUMAN PANCREATIC CANCER CELL LINE (HGC‐25)
Authors: Akagi Tadaatsu , Kimoto Tetsuo
Published: December 2008

A floating cell line (HGC‐25) was established from the metastatic ascitic fluid of a human pancreatic adenocarcinoma of ductal cell origin. The cell line was characterized by the growth in suspension with a doubling time of 15.6 hr, a high cloning efficiency in soft agar and a modal chromosome number of 72. Electron microscopic examination revealed terminal bars in a small number of cells. Production of mucin and immunoglobulins and phagocytosis were not demonstrated. Heterotransplantation of the cells produced tumors, being undifferentiated carcinoma histologically. These characteristics mentioned above confirm that HGC‐25 cell line is a human pancreatic cancer ceU line.
PULMONARY CHANGES IN CONGENITAL HEART DISEASE OF CHILDHOOD
Authors: ITOH Hiroshi , OGAWA Kyoichi , TORIYAMA Akira , KIMURA Ken , CHEONG Wong Ki , MITO Hisashi
Published: December 2008

One hundred and fifty lungs from the cases below 15 years of age with various congenital heart diseases and 80 controls were used for histometrical and histological studies. Gases with congenital heart disease were divided into two groups of the increased and the decreased pulmonary blood flow. In the former group, the thickness of the pulmonary arterial media was the same as that of controls in the neonatal period, and though the wall thickness gradually decreased in a pattern seen In controls, the thickness was constantly larger than that of controls. In some cases, the media increased gradually within 6 months after birth. Pneumonia and massive pulmonary hemorrhage were seen in a higher incidence in autopsy cases. Pneumonia In younger infants was histologically characteristic and possibly more correlated to their death. In the latter group, most of the cases were with the thinner medias of the pulmonary arteries. Massive pulmonary hemorrhage was not common in the latter group.
ELEKTRONENMIKROSKOPISCHE UND ELEKTRONENMIKROSKOPISCH‐HISTOCHEMISCHE UNTERSUCHUNGEN UBER NEBENNIERENRINDE DER RATTE MIT AMINOGLUTÄTHIMID (ELIPTEN, CIBA) ÜND UBER IHREN REPARATURPROZESS.
Authors: Itoh Gen
Published: December 2008

Amlnoglutethimide blocks the conversion of free cholesterln to D́5‐pregnenolone. It's effects on the fasciculata cells were studied electron microscopically in Wistar rats fed with this chemical for a maximum of seven days. The animals surviving after this treatment were also examined to follow the process of restoration from the damage. Four to seven days after the initiation of the feeding, the mitochondria were enlarged, variously deformed and contained intramitochondrial vacuoles. The lipid droplets were increased in size. Three to four days after withdrawal of amlnoglutethimide, so‐called “Intramitochondrial mitochondria” appeared. The picture suggesting division of the mitochondrial matrx was also occasionally encountered. These features suggested formation of new mitochondria from preexisting mitochondria. The mltohchondrla resumed their normal shape and appearance starting about ten days after withdrawal of amlnoglutethimide. The electron microscopic histochemlcal method demonstrated tubular crystals of digitonin‐cholesterid in the intramitochondrial vacuoles as well as in the lipid droplets 4 days after the initiation of the feeding. The withdrawal of amlnoglutethimide was followed with disappearance of these crystals from the sites of deposition.
MESENCHYMAL CHONDROSARCOMA A CASE REPORT WITH AN ULTRASTRUCTURAL STUDY and REVIEW OF JAPANESE LITERATURES
Authors: Mikata Atsuo , Iri Hisami , Inuyama YuMo
Published: December 2008

A case of mesenchymal chondrosarcoma occurring in the left maxilla of a 30‐year‐old Japanese male was reported. Electron microscopic observation confirmed transition of undifferentiated cells to chondrocytes and calcification in similar manner as previously described with physiological processes. Matrix vesicles were thought to be the cytoplasmic products of tumor chondrocytes. Electron‐dense finely granular deposits resembling the organic matrix of cartilagenous calcification were seen within the intercellular matrix. Matrix vesicles were also closely related to these deposits. Japanese cases of this rare tumor were reviewed and 8 cases, Including the present case as 6th among them, were accepted. Clinical characteristics were similar to those of a series reported by SALVADOR et al.
PATHOLOGY OF NEOPLASMS AND OTHER LESIONS INDUCED IN RATS WITH MOLONEY MURINE SARCOMA VIRUS
Authors: Hirano Takahiro , Miyajima Hiroaki , Kaziwara Kyo , Watanabe Tasuku
Published: December 2008

Moloney murine sarcoma virus (M‐MSV) was injected directly into the fetuses of Sprague‐Dawley rats during the late stage of gestation and into the neonates within 24 hours after birth. Ninety rats developed 188 neoplastic lesions during the 8‐week period of observation. Nearly all of the neoplasms were of mesenchymal derivation. Sixty percent of these neoplasms revealed more complex histologic features than those previously reported for neoplasms induced in rodents with M‐MSV and were designated “malignant mesenchymoma” which developed preferentially in the proximal parts of the extremities, distant from the inoculation site. Rhabdomyosarcoma and osteosarcoma which developed in a pure form at the various sites were the next most common tumor type. Osteosarcoma developing in a pure form and as a component of malignant mesenchymoma in the humerus and femur was comparable to that of juxtacortical osteosarcoma of man. The development of excessive bones were observed in the forelimb and/or hind leg, suggesting a type of skeletal malformation. The reaction to M‐MSV merits attention as a model for the study of an osteosarcoma and malignant mesenchymoma as well as rhabdomyosarcoma and also for the study of viral teratogenesis in man, as “rubella syndrome”.
AN UNUSUAL CASE WHICH BEGAN WITH SUBCUTANEOUS PANNICULITIS FOLLOWED BY FEVER, SEVERE HEPATIC INVOLVEMENT AND HYPERLIPIDEMIA
Authors: Miyasaki Kichihei , Ooiso Yutaka , Nakamura Isao , Oimomi Munetada , Tai Tadashi , Saito Kunihiko
Published: December 2008

A 25‐year‐old man was at first dermatologically suspected as suffering from Weber‐Christian syndrome because of subcutaneous panniculitis, but his skin lesions disappeared completely during the course. Hyperlipidemla, disturbances In liver function, and leukemoid reaction became remarkable, and he died of subarachnoid hemorrhage eleven months after onset. Necropsy revealed subarachnoid hemorrhage at the base of the brain, lipogranulomatous and inflammatory lesions in the upper lobe of the left lung, a remarkable fatty liver, splenomegaly, pericarditis, and foam cells in the spleen, liver, and bone marrow. A comparison with 57 autopsy cases of Weber‐Christian syndrome reported in the literature showed our case to be an exceptional instance of Weber‐Christian syndrome, If the present case is not to be regarded as a different disease entity. ACTA PATH. JAP. 27:213–224, 1977.
NOONAN SYNDROME WITH HYPERTROPHIC OBSTRUCTIVE CARDIOMYOPATHY
Authors: Tanimura Akira , Hayashi Itsuro , Adachi Kyo , Nakashima Teruyuki , Ota Katsutoshi , Toshima Hidenori
Published: December 2008

A case of a 20 years old female who had Noonan syndrome associated with obstructive cardiomyopathy was presented. It is well known that Noonan syndrome is frequently complicated with cardiac anomaly, and although no autopsies were performed two cases have been diagnosed clinically as Noonan syndrome accompanied with idiopathic cardiomyopathy in our country. The present case would be the first autopsied case in Japan of Noonan syndrome associated with idiopathic obstructive cardiomyopathy.
VIRAL PLACENTITIS ‐ A CASE REPORT ‐
Authors: Saito Kiyoko , Koizumi Fumitomo , Sumiyoshi Yuichi
Published: December 2008

A case of viral placentitis, In a 20 year‐old pregnant woman suffering from chronic glomerulonephritis, was presented. In light microscopic findings of the placenta, many necrotic foci with intranuclear Inclusion‐ bearing cells revealed in chorionic villi and by electron microscopy numerous immature and mature virus particles, probably herpes simplex were proved in intranuclear inclusion‐bearing cells.
CHRONIC INTESTINAL BLEEDING DUE TO ARTERIOVENOUS MALFORMATION OF THE COLON
Authors: Matsumoto Masao , Ishigueo Minoru , Kato Muneaki , Sugihara Takao
Published: December 2008

A case of chronic intestinal bleeding due to arteriovenous malformation of the ascending colon was reported. Vascular abnormalities of the bowel represent the most common cause of gastrointestinal bleeding, the source of which could not be disclosed by conventional diagnostic procedures; selective abdominal angiography is the only most reliable technique in such cases. An acquaintance with these conditions may be of importance in the search for the cause of intestinal hemorrhages of otherwise obscure origin, and more attention should be paid to the clinicopathologic features of this entity.
MEDIAN RHOMBOID GLOSSITIS ASSOCIATED WITH AMYLOID DEPOSITION
Authors: Yamaoka Yutaka , Suzuki Atsumi , Hatakeyama Setsuko , Noda Mieko , Hiraga Mitsugu , Sekiyama Saburo
Published: December 2008

There are some controversies over the pathogenesis and clinical features of median rhomboid glossitis. A case of median rhomboid glossitis associated with amyloid deposition was presented. Clinically there was no organ involved in amyloid other than the tongue. It was suspected that median rhomboid glossitis occurred first and that amyloid was induced later.
SITE OF ORIGIN OF 2,7‐FAA‐INDUCED RAT LEUKEMIA
Authors: Takayama Shojiro , FUjiwara Mutsunori
Published: December 2008

Oral administration of N,N'‐2,7‐fluorenylenebis‐acetamide (2,7‐PAA) induces mature granulocytic and erythroblastic leukemia in rats. We compared the primary site of mature granulocytic leukemia with that of erythroblastic leukemia. The nodular foci of solitary lesions and conspicuously large foci of scattered lesions were considered to be the primary site. They appeared mainly in the bone marrow of various bones. The primary site of mature granulocytic leukemia appeared more frequently in short bones than in long bones. On the contrary, that of erythroblastic leukemia appeared more frequently in long bones than in short bones. The origin of mature granulocytic leukemia seems to be concerned with bone tissue, while erythroblastic leukemia may have little relation with bone tissue.
HEMANGIOSARCOMA OF THE BREAST—CASE REPORT WITH SCANNING ELECTRON MICROSCOPIC STUDY—
Authors: Hamazaki Mikage , Tanaka Toshio
Published: December 2008

Hemangiosarcoma of the breast in a 22‐year‐old female was presented. She died of massive pulmonary metastasis and apparent internal hemorrhage from the involved liver by metastasis after 40 months of the illness. Intense scanning electron microscopic (SEM) study was undertaken on this lesion. At the same time, in order to compare SEM findings of angiosarcoma with several other benign disorders, the following lesions were chosen, namely, chronically‐inflamed veins in the gastric submucosa, benign cavernous hemangiomas of the skin and liver, and organizing thrombi with papillary structures. Some of these lesions exhibited focal similarities in surface structures to those from angiosarcoma. Besides various characteristics by SEM in angiosarcoma, cell clusters protruding into the vascular cavity were found to be the most unique feature which we have never observed in any other lesions.
HEPATIC SARCOMA ASSOCIATED WITH HEPATOMA
Authors: Nagamine Yoshiaki , Sasaki Kohsuke , Kaku Kohhei , Takahashi Manabu
Published: December 2008

An autopsy case with simultaneous occurrence of hepatoma and spindle cell sarcoma in the liver was presented. These tumors arose in the left and right lobes and showed no continuity. The sarcoma metastasized in hilar lymph node of the liver and in the cervical and lumbar vertebrae, but no metastasis of the hepatoma was found except in the opposite lobe of the liver. Literature on such combinations was briefly reviewed.
IMMUNOHISTOCHEMICAL OBSERVATION OF LYSOZYME IN MACROPHAGES AND GIANT CELLS IN HUMAN GRANULOMAS
Authors: Yamashita Kiyoaki , Iwamoto Toshiyuki , Iijima Soichi
Published: December 2008

Lysozyme activity of macrophages and giant cells in various human granulomas were examined with immunoperoxidase bridge method in tissue sections. Various numbers of epithelioid cells and giant cells of epithelioid cell granulomas of tuberculosis, sarcoidosis and Crohn's disease exhibited intense granular cytoplasmic lysozyme activity. Foreign body granulomas induced with various substances showed negative or faintly positive lysozyme stain. Macrophages and giant cells of aspergillus granuloma associated with thymus hypoplasia and T‐cell depression contained no lysozyme. The results suggest that cell‐mediated immunology plays an important role for the lysozyme synthesis of macrophages in granuloma.
IMMUNOHISTOCHEMICAL OBSERVATION OF LYSOZYME IN MACROPHAGES IN LEPROSY
Authors: Yamashita Kiyoaki , Iwamoto Toshiyuki , Iijima Soichi
Published: December 2008

Lysozyme activities of skin granulomas of 24 patients in leprosy were studied. Lepra cells of all 15 lepromatous leprosy showed strong lysozyme activity in cytoplasma. In the specimens stained with lysozyme and Ziehl‐Neelsen's carbolfuchsin double stain conspicuous lysozyme activity around M. leprae were observed. One borderline case was negative. Lysozyme of epithelioid cells and giant cells of 10 tuberculoid types were completely negative. These results suggest that lysozyme plays only a small role in the disposal of M. leprae in macrophages and other mechanisms than bacteriolytic function of lysozyme are responsible for the defence against these bacilli.
INFANTILE CONVULSION SUSPECTED OF PYRIDOXINE RESPONSIVE SEIZURES
Authors: Miyasaki Kichihei , Matsumoto Junji , Murao Shinichi , Nakamuba Kazushige , Yokoyama Sumiyoshi , Hayano Masayoshi , Nakamura Hajime
Published: December 2008

A 6‐month‐old boy, having suffered repeated convulsive seizures since birth, died of severe epilepsy, the disease remaining unknown. However, when a brother, born after the death of the present case, displayed the same clinical symptoms and was diagnosed clinically as having pyridoxine dependent convulsion, the deceased became suspected of having been a case of familial pyridoxine dependency. Pathologically, bilateral cerebellar hemorrhages were found accompanied by subdural and subarachnoidal hemorrhages. Histologically, neuronal loss and diffuse astrocyte hyperplasia were found hi the brain: Beside marginal gliosis, proliferations of astroglia and glial fibers were found, especially hi the pons, medulla oblongata, and cervical cord, although the fibrillary gliosis may be representative for a physiological intermediate state. Foamy cells were found in the lymph nodes, but not hi the brain. Observed hi the kidneys were substances deposited hi the glomeruli and Bowman's capsules and materials hi the tubular lumen.
DISSEMINATED ATYPICAL MYCOBACTERIOSIS IN A CHILD
Authors: Yun Kankatsu , Matsuo Takeshi , Kohno Shigeru , Ikeda Takayoshi , Sakai* Masayoshi , Yoshimoto* Masaaki , Nakashita** Yoshio
Published: December 2008

A case of disseminated mycobacterial disease caused by mycobacterium sculoflaceum is presented in a three‐year‐old female. Atypical acid‐fast organisms were found in cultures from the tissue of lymph node and bone marrow, gastric juice and feces. Histologically the mesenterlc lymph nodes contained numerous large macrophages which had large numbers of acid‐fast bacilli. Gaseation necrosis was absent. Distinguishing features are the extensive involvement of lymph nodes and bones and the preference for intra‐cellular presence of acid‐fast bacilli.

Up to the present in Japan there are only 17 reported cases of atypical mycobacteriosis in children.
LETTERER‐SIWE DISEASE WITH LIPID‐CONTAINING VACUOLATED CELLS
Authors: Kawai Kioko , Harada Tomoyuki , Matsukuma Hidetaka , Nakashita* Yoshio
Published: December 2008

A case of Letterer‐Siwe disease of a 1‐year‐2‐month‐old boy, which clinicopathologically satisfied 7 out of the 8 characteristics of the disease by Siwe's criteria, excluding pathologic changes in the bone is reported.

The vacuolated histiocytes appeared in the promerating reticuloendothelial cells in this case; the cells were positive by general fat reaction; the fats consisted of cholesterol fats, phospholipids and neutral lipid. Electron microscopy showed the occurrence of lipid droplets with cholesterol crystals and atypical form of Langerhans cell granule. Granulomatous changes were found in the lymph nodes and spleen, and this case was also simllar in part to chronic granulomatous disease; however, celoid and lipofuscin pigments were negative in the proliferating cells. Cytomegalic inclusion disease as a complication was found in the lungs and intestine, and bilirubin stones in the liver.
TUMOR CELL PHAGOCYTOSIS AND CYTOTOXICITY OF LYMPHOBLASTOID CELLS FOLLOWING CONCANAVALIN A TREATMENT
Authors: Kimoto Tetsuo , Hyodoh Fuminori , Nishitani Koji , Namba Masayoshi , Ueki Ayako
Published: December 2008

Cell‐to‐cell interaction was investigated in various malignant tumor cells (human ovarial tumor, lung cancer, carcinoma of larynx and hamster melanoma cell) and in human lymphoblastoid cells (T‐cell (MOLT‐4 cell), thymoma cells and B‐cells (Burkitt lymphoma cell)). Live lymphoblastoid cells did not adhere to the cell surfaces of tumor cells nor the lymphoblastoid cells were ingested by tumor cells wihout immunologic and specific treatment. Tumor cells as well as T‐cells and B‐cells had receptors to concanavalin A on their surfaces, and they showed marked cell binding of tumor cells and lymphoblastoid cells. Moreover, tumor cells that phagocytized lymphoblasts underwent marked cell destruction within 4 hours of cell binding. The cytolytic mechanism of the target tumor cell was probably related to contact with the lymphoblastoid cells and was increased by ingestive activity, and metabolic disturbance by lymphotoxin in tumor cells.
CHILDHOOD CORONARY SCLEROSIS
Authors: Sakurai Isamu , Tosaka Akira , Yamada Tsutomu , Kuwahara Takeichiro , Masubuchi Kazuo
Published: December 2008

Childhood coronary sclerosis was studied histologically, histochemically immunohistochemically and biochemically on 60 autopsy cases aged from new‐born to 20 years. Intimal thickening often started even in the newborn babies particularly at branching or bending points of the arteries, where fibrocellular proliferation with acid mucopolysaccharide accumulation was the main histological feature. Microthrombi were occasionally demonstrated even in infants, and fatty streaks appeared generally in the second decade. Microthrombi were usually smaller and fewer than fatty streaks. Betalipoprotein and fibrinogen infiltrations into intima were proven in some cases. Mural vascularization was occasionally observed especially in the areas beneath intimal thickening. Pressure effect of the blood stream and anatomically specific points in the arteries seemed to be important as initial factors of coronary sclerosis, where plasma constituents might pass in an easier manner into intimas by increased permeability. Secondarily mural capillaries might play some role in developing atheromas which would produce an eccentrically located luminal obstruction later in adulthood, by local circulatory disturbances within the arterial walls.
SINUS HISTIOCYTOSIS WITH MASSIVE LYMPHADENOPATHY (RO SAI AND DORFMAN) AND SIGNIFICANT SKIN INVOLVEMENT
Authors: Tanaka Noboru , Asao Takeshi
Published: December 2008

Clinicopathological features of this female patient described here, showing unusual and extremely marked sinus histiocytosis of lymph nodes with considerable systemic lymphadenopathy, accompanying fever as well as acceleration of erythrocyte sedimentation rate fairly well coincided with the symptoms of “Sinus Histiocytosis With Massive Lymphadenopathy” (Rosai and Dorfman). The onset of the present case was in 1966 when the patient was 10 years of age, and she has been followed‐up for over 10 years. While persisting to display active clinical manifestations, immunological abnormality with thymus involution was demonstrated — deterioration of cell‐mediated immunity, but with non‐remarkable humoral immunological data, except for hypergamma‐globulinemia with elevation of IgG. In 1973 lymphadenopathy as well as skin eruption extended over the extremities and abnormalities of hematological and serological nature began to improve and at the present date the patient is uneventful. This is the first recorded case of “Sinus Histiocytosis With Massive Lymphadenopathy” (Rosai and Dorfman) in Japan. Considerable skin involvement in the upper and lower extremities was a significant feature and unique in this case.
FOUR AUTOPSY CASES OF NEONATAL GIANT CELL HEPATITIS DIED SUDDENLY AND UNEXPECTEDLY FROM INTRACRANIAL HEMORRHAGE
Authors: Sugai Masayoshi , Kono Rin , Hiroki Tadakazu , Kunita Yukie , Tokudome Shogo
Published: December 2008

The authors present four cases of neonatal giant cell hepatitis who died suddenly and unexpectedly from intracranial hemorrhage, autopsied recently at the Tokyo‐to Medical Examiner Office. This series consisted of one male and 3 female infants ranging from 37 to 52 days of age. The cause of death of all cases was thought to be massive intracranial hemorrhage. Referring to the connection between intracranial hemorrhage and neonatal giant cell hepatitis, it was assumed that the former must have occurred due to the hemorrhagic tendencies of the latter, superimposing to the hemorrhage at birth trauma of the head. As to the formation of multinucleated giant cell, it is considered to be of degenerative nature of hepatocytes, i.e. the giant cell is formed by fusion of hepatocytes as a result of the disappearance of bile canaliculi, although the etiology of this hepatitis is still unknown.
DIFFERENTIATION OF MOUSE T‐LYMPHOCYTES 1. CHARACTERIZATION OF THYMOCYTE PRECURSORS IN BONE MARROW BY ONE G VELOCITY SEDIMENTATION
Authors: Nakao Satoshi , Namba Yuziro , Hanaoka Masao
Published: December 2008

Bone marrow cells were transferred into 700 RX‐irradiated syngeneic mice, and reganeration of the thymus was demonstrated by determining the total amount of DNA in the thymus. The amount of DNA in the regenerated thymus 9 days after X‐irradiation paralleled the number of cells injected, the range being 0 to 106 cells. These results indicate that regeneration during this period reflects the proliferation of thymocyte precursors existing in bone marrow cells, therefore such can be used as an indicator to determine the number of thymocyte precursors.

Thymocyte precursors were separated from spleen colony forming cells and soft agar colony forming cells by one G velocity sedimentation. Thymocyte precursors (more than 4.3 mm/hour sedimentation rate) sedimented faster than did spleen colony forming cells (peak at about 3.0 mm/hour) and Thy‐1 antieen was already evidenced on the cell surface as demonstrated by complement dependent cytotoxicity. Soft agar colony forming cells also sedimented faster, suggesting that cells destined to become one type of cell are larger than multi‐potential stem cells.
A FREEZE‐FRACTURE STUDY OF TUBULAR MYELIN IN FETAL RABBIT LUNG
Authors: Manabe Toshiaki
Published: December 2008

A freeze‐fracture replication study was undertaken to clarify the relationship between intracellular lamellar bodies of type II alveolar epithelial cells and intra‐alveolar contents in late gestation rabbit fetuses. The interior of the inclusion bodies was composed of multiple stacks and/or whorls of membranes devoid of membrane‐associated particles, while the limiting membrane of intracellular lamellar inclusion bodies was studded with membrane‐associated particles of 150Å diameter.

The intra‐alveolar contents were comprised of two components; spherical bodies, and tubular elements. Spherical bodies were identical to the internal contents of the lamellar bodies and also were devoid of membrane‐associated particles. Tubular elements mostly appeared rectangular on cross‐fractured faces, but triangular and hexagonal forms could also be seen. These tubules rested on the surfaces of the spherical bodies and appeared to be formed of the outer lipid monolayer of spherical bodies. The present observations suggest that the tubular element of the alveolar contents is formed through the interaction between the discharged lamellar body content and the alveolar fluid.
STUDY ON THE ULTRASTRUCTURE AND ACETYLCHOLINESTERASE ACTIVITY IN VON RECKLINGHAUSEN'S NEUROFIBROMATOSIS
Authors: Kamata Yoshimasa
Published: December 2008

The cutaneous nodules obtained from seven patients with von Reckling‐hausen's neurofibromatosis were investigated by electron microscopy, and ultrastructural localization of acetylcholinesterase activity was demonstrated in the nerve fibers of this tumor for the first time using Karnovsky's thiocholine method. The enzymatic activity was mainly found in unmyelinated fibers, exactly associated with their axonal membranes, the interspace between the apposing axonal and Schwann cell membrane, and some different mesaxons, which indicated their cholinergic nature. Almost all myelinated fibers and some unmyelinated fibers did not possess the activity. The relationship between axon and Schwann cell was quite similar to that of normal peripheral nervous system, but two striking alterations of the nerves existed: One is the dissociation of unmyelinated fibers, and the other is the degenerative changes of the axon and the myelin sheath. As the evidence of schwannian proliferation, onion bulb formations and collagen pockets were observed. Some signs of fibroblastic proliferation were also found.

From the present study and the review of the literature, the probable histogenesis of this disease was discussed.
LIGHT AND ELECTRONMICROSCOPIC OBSERVATIONS ON HEPATIC HEMATOPOIESIS OF HUMAN FETUSES
Authors: Enzan Hideaki , Takahashi Hiroshi , Kawakami Masahito , Yamashita Seiki , Ohkita T.
Published: December 2008

Focusing on hematopoiesis, especially granulocytopoiesis, livers from ten human fetuses between 7 to 13 weeks of gestation were observed with light and electron microscpe. In the hepatic mesenchymal tissue variable number of granulocytes in varying stage of maturation were found from 10 to 13 weeks of gestation. The granules of neutrophils in human fetal livers were classified into three distinct types. The first granules appeared in myeloblasts. The second granules, possibly formed in Golgi complex, were more larger and observed after the promyelocytic stage. The third granules, predominant in mature neutrophils, were pleomorphic. Other than granulo‐cytic, erythrocytic and stromal cells, unclassifiable cells were occasionally encountered. The role of mesenchymal tissues in the granulocytopoiesis, the development of fetal neutrophfls and the problem of hematopoietic stem cells were discussed.
GROWTH AND CYTOGENETIC CHARACTERISTICS OF NICKEL SULPHIDE‐INDUCED RHABDOMYOSARCOMAS IN RATS
Authors: Yamashiro Shigeto , Gilman J.P.W. , Basrur P.K. , Abandowitz H.M.
Published: December 2008

Rhabdomyosarcomas induced by single intramuscular injections of nickel sulphide (Ni3S2) in Fischer and Hooded rats were cultured in vivo and and in vitro to study their growth characteristics and chromosomal constitution. The tumor cell suspensions cultured in vitro exhibited more myogenic differentiation on the coverslips than those cells grown in vivo in diffusion chambers. A characteristic feature of in vivo cultures was the appearance of microclusters which resembled the primary tumors.

Chromosome analyses of primary tumors revealed that a majority of these had a modal number in the diploid or near diploid range. Fischer rat primary tumor cells exhibited abnormal configurations including rings, dicentrics and triradials. A comparison of the chromosome make‐up of the primary tumors and their metastases was performed on four sets of tumors. Three out of four metastases examined showed the diploid chromosome make‐up characteristic of the primary tumors suggesting that the tumors with the diploid or near diploid chromosome constitution are more likely to produce metastases.
BRAIN TUMORS INDUCED IN HAMSTERS BY INTRACEREBRAL INOCULATION OF SR‐RSV‐INFECTED EMBRYONIC BRAIN CELLS
Authors: Nakazato Yoichi , Ishida Yoichi
Published: December 2008

Brain tumors were induced in Syrian hamsters by intracerebral inoculation of brain cells which were obtained from 12‐day old syngeneic hamster and infected with Schmidt‐Ruppin strain of Rous sarcoma virus (SR‐RSV) in vitro. A total of 212 tumors were developed in all 25 recipients within 19 to 125 days alter transplantation. On the basis of light and electron microscopic study, they were classified into four main groups: astrocytoma (45.8%). pleomorphic glioma (50.0%), sarcoma (3.8%). unclassified (0.4%). The morphological features of these tumors were described, and the advantages of this brain tumor model were discussed.
FETAL CASE OF XERODERMA PIGMENTOSUM
Authors: Okayasu Isao , Satoh Yoshiaki , Irimajiri Toshikatsu , Mitoh Yoshihiro
Published: December 2008

Fetal autopsy case of xeroderma pigmentosum was reported. This male fetus of 24 gestational weeks was prenatally diagnosed as xeroderma pigmentosum by detecting the DNA‐repair defect of the amniotic fluid cells. Autopsy revealed not only maldevelopment of the fetus (stillbirth) in general for the standard, but also showed slight developmental retardation of various organs including kidneys and lungs, which could be examined by microscopic analysis. It was suggested that abnormality of the somatic organs began to appear at the fetal stage in this case of xeroderma pigmentosum.