bluuebunny/crossref_metadata_2025 huggingface dataset:
Addressing the Compartmentalization of Specific Integrin Heterodimers in Mouse Sperm
Authors: Frolikova Michaela , Valaskova Eliska , Cerny Jiri , Lumeau Audrey , Sebkova Natasa , Palenikova Veronika , Sanchez-Hernandez Noemi , Pohlova Alzbeta , Manaskova-Postlerova Pavla , Dvorakova-Hortova Katerina
Published: February 2019

Integrins are transmembrane cell receptors involved in two crucial mechanisms for successful fertilization, namely, mammalian intracellular signaling and cell adhesion. Integrins α6β4, α3β1 and α6β1 are three major laminin receptors expressed on the surface of mammalian cells including gametes, and the presence of individual integrin subunits α3, α6, β1 and β4 has been previously detected in mammalian sperm. However, to date, proof of the existence of individual heterodimer pairs in sperm and their detailed localization is missing. The major conclusion of this study is evidence that the β4 integrin subunit is expressed in mouse sperm and that it pairs with subunit α6; additionally, there is a detailed identification of integrin heterodimer pairs across individual membranes in an intact mouse sperm head. We also demonstrate the existence of β4 integrin mRNAs in round spermatids and spermatogonia by q-RT-PCR, which was further supported by sequencing the PCR products. Using super-resolution microscopy accompanied by colocalization analysis, we located integrin subunits as follows: α6/β4-inner apical acrosomal membrane and equatorial segment; α3, α6/β1, β4-plasma membrane overlaying the apical acrosome; and α3/β1-outer acrosomal membrane. The existence of α6β4, α3β1 and α6β1 heterodimers was further confirmed by proximity ligation assay (PLA). In conclusion, we delivered detailed characterization of α3, α6, β1 and β4 integrin subunits, showing their presence in distinct compartments of the intact mouse sperm head. Moreover, we identified sperm-specific localization for heterodimers α6β4, α3β1 and α6β1, and their membrane compartmentalization and the presented data show a complexity of membranes overlaying specialized microdomain structures in the sperm head. Their different protein compositions of these individual membrane rafts may play a specialized role, based on their involvement in sperm-epithelium and sperm-egg interaction.
Potential of Novel Sequence Type of Burkholderia cenocepacia for Biological Control of Root Rot of Maize (Zea mays L.) Caused by Fusarium temperatum
Authors: Tagele Setu Bazie , Kim Sang Woo , Lee Hyun Gu , Lee Youn Su
Published: February 2019

In this study, two Burkholderia strains, strain KNU17BI2 and strain KNU17BI3, were isolated from maize rhizospheric soil, South Korea. The 16S rRNA gene and multilocus sequence analysis and typing (MLSA-MLST) were used for the identification of the studied strains. Strain KNU17BI2, which belonged to Burkholderia cenocepacia, was of a novel sequence type (ST) designated ST-1538, while strain KNU17BI3 had a similar allelic profile with the seven loci of Burkholderia contaminans strain LMG 23361. The strains were evaluated in vitro for their specific plant growth promoting (PGP) traits, such as zinc solubilization, phosphate solubilization, ammonia production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, indole acetic acid (IAA) production, siderophore, and hydrolytic enzyme activity. Interestingly, the strains exhibited a positive effect on all of the tested parameters. The strains also showed broad-spectrum antifungal activity against economically important phytopathogens in the dual culture assay. Furthermore, the strains were evaluated under greenhouse conditions for their in vivo effect to promote plant growth and to suppress the root rot of maize that is caused by Fusarium temperatum on four Korean maize cultivars. The results of the greenhouse study revealed that both of the strains were promising to significantly suppress fusarium root rot and enhance plant growth promotion on the four maize cultivars. This study, for the first time, reported in vitro antifungal potential of B. cenocepacia of novel ST against economically important plant pathogens viz., F. temperatum, Fusarium graminearum, Fusarium moniliforme, Fusarium oxysporum f.sp. melonis, Fusarium subglutinans, Phytophthora drechsleri, and Stemphylium lycopersici. This is also the first report of zinc solubilization by B. cenocepacia. Moreover, the present research work reports, for the first time, about the potential of B. cenocepacia and B. contaminans to control the root rot of maize that is caused by F. temperatum. Therefore, we recommend further studies to precisely identify the bioactive chemical compounds behind such activities that would be novel sources of natural products for biological control and plant growth promotion of different crops.
Heme Oxygenase 1 Impairs Glucocorticoid Receptor Activity in Prostate Cancer
Authors: Leonardi Daiana B. , Anselmino Nicolás , Brandani Javier N. , Jaworski Felipe M. , Páez Alejandra V. , Mazaira Gisela , Meiss Roberto P. , Nuñez Myriam , Nemirovsky Sergio I. , Giudice Jimena , Galigniana Mario , Pecci Adalí , Gueron Geraldine , Vazquez Elba , Cotignola Javier
Published: February 2019

Glucocorticoids are used during prostate cancer (PCa) treatment. However, they may also have the potential to drive castration resistant prostate cancer (CRPC) growth via the glucocorticoid receptor (GR). Given the association between inflammation and PCa, and the anti-inflammatory role of heme oxygenase 1 (HO-1), we aimed at identifying the molecular processes governed by the interaction between HO-1 and GR. PCa-derived cell lines were treated with Hemin, Dexamethasone (Dex), or both. We studied GR gene expression by RTqPCR, protein expression by Western Blot, transcriptional activity using reporter assays, and nuclear translocation by confocal microscopy. We also evaluated the expression of HO-1, FKBP51, and FKBP52 by Western Blot. Hemin pre-treatment reduced Dex-induced GR activity in PC3 cells. Protein levels of FKBP51, a cytoplasmic GR-binding immunophilin, were significantly increased in Hemin+Dex treated cells, possibly accounting for lower GR activity. We also evaluated these treatments in vivo using PC3 tumors growing as xenografts. We found non-significant differences in tumor growth among treatments. Immunohistochemistry analyses revealed strong nuclear GR staining in almost all groups. We did not observe HO-1 staining in tumor cells, but high HO-1 reactivity was detected in tumor infiltrating macrophages. Our results suggest an association and crossed modulation between HO-1 and GR pathways.
Low-Frequency Mutational Heterogeneity of Invasive Ductal Carcinoma Subtypes: Information to Direct Precision Oncology
Authors: Myers Meagan B. , McKim Karen L. , Banda Malathi , George Nysia I. , Parsons Barbara L.
Published: February 2019

Information regarding the role of low-frequency hotspot cancer-driver mutations (CDMs) in breast carcinogenesis and therapeutic response is limited. Using the sensitive and quantitative Allele-specific Competitor Blocker PCR (ACB-PCR) approach, mutant fractions (MFs) of six CDMs (PIK3CA H1047R and E545K, KRAS G12D and G12V, HRAS G12D, and BRAF V600E) were quantified in invasive ductal carcinomas (IDCs; including ~20 samples per subtype). Measurable levels (i.e., ≥ 1 × 10−5, the lowest ACB-PCR standard employed) of the PIK3CA H1047R, PIK3CA E545K, KRAS G12D, KRAS G12V, HRAS G12D, and BRAF V600E mutations were observed in 34/81 (42%), 29/81 (36%), 51/81 (63%), 9/81 (11%), 70/81 (86%), and 48/81 (59%) of IDCs, respectively. Correlation analysis using available clinicopathological information revealed that PIK3CA H1047R and BRAF V600E MFs correlate positively with maximum tumor dimension. Analysis of IDC subtypes revealed minor mutant subpopulations of critical genes in the MAP kinase pathway (KRAS, HRAS, and BRAF) were prevalent across IDC subtypes. Few triple-negative breast cancers (TNBCs) had appreciable levels of PIK3CA mutation, suggesting that individuals with TNBC may be less responsive to inhibitors of the PI3K/AKT/mTOR pathway. These results suggest that low-frequency hotspot CDMs contribute significantly to the intertumoral and intratumoral genetic heterogeneity of IDCs, which has the potential to impact precision oncology approaches.
Down-Regulation of Astrocytic Kir4.1 Channels during the Audiogenic Epileptogenesis in Leucine-Rich Glioma-Inactivated 1 (Lgi1) Mutant Rats
Authors: Kinboshi Masato , Shimizu Saki , Mashimo Tomoji , Serikawa Tadao , Ito Hidefumi , Ikeda Akio , Takahashi Ryosuke , Ohno Yukihiro
Published: February 2019

The dysfunction of astrocytic inwardly rectifying potassium (Kir) 4.1 channels, which mediate the spatial potassium-buffering function of astrocytes, is known to be involved in the development of epilepsy. Here, we analyzed the Kir4.1 expressional changes in Leucine-Rich Glioma-Inactivated 1 (Lgi1) mutant rats, which is a model of autosomal dominant lateral temporal lobe epilepsy in humans, to clarify the role of astrocytic Kir4.1 channels in Lgi1-related epileptogenesis. Priming acoustic stimulation (at postnatal day 16) conferred seizure susceptibility on Lgi1 mutant rats, which evoked audiogenic seizures with test stimulation at eight weeks. In the seizure-susceptible Lgi1 mutant rats (before test stimulation), astrocytic Kir4.1 expression was down-regulated region-specifically in the cerebral cortex, hippocampus, and amygdala. In addition, prophylactic treatments of Lgi1 mutant rats with valproic acid (VPA, 30 mg/kg and 200 mg/kg) for two weeks prevented both the development of seizure susceptibility and the down-regulation of Kir4.1 expression in astrocytes. The present study demonstrated for the first time that the astrocytic Kir4.1 expression was reduced in the Lgi1-related seizure model, suggesting that the down-regulation of Kir4.1 channels in astrocytes is involved in audiogenic epileptogenesis caused by Lgi1 mutation. In addition, VPA seemed to have a prophylactic effect on Lgi1-related seizures.
Histological and Cytological Characterization of Anther and Appendage Development in Asian Lotus (Nelumbo nucifera Gaertn.)
Authors: Zhang Dasheng , Chen Qing , Liu Qingqing , Liu Fengluan , Cui Lijie , Shao Wen , Wu Shaohua , Xu Jie , Tian Daike
Published: February 2019

The lotus (Nelumbo Adans.) is a perennial aquatic plant with important value in horticulture, medicine, food, religion, and culture. It is rich in germplasm and more than 2000 cultivars have been cultivated through hybridization and natural selection. Microsporogenesis and male gametogenesis in the anther are important for hybridization in flowering plants. However, little is known about the cytological events, especially related to the stamen, during the reproduction of the lotus. To better understand the mechanism controlling the male reproductive development of the lotus, we investigated the flower structure of the Asian lotus (N. nucifera). The cytological analysis of anther morphogenesis showed both the common and specialized cytological events as well as the formation of mature pollen grains via meiosis and mitosis during lotus anther development. Intriguingly, an anatomical difference in anther appendage structures was observed between the Asian lotus and the American lotus (N. lutea). To facilitate future study on lotus male reproduction, we categorized pollen development into 11 stages according to the characterized cytological events. This discovery expands our knowledge on the pollen and appendage development of the lotus as well as improving the understanding of the species differentiation of N. nucifera and N. lutea.
Genomic Analysis of Shewanella sp. O23S—The Natural Host of the pSheB Plasmid Carrying Genes for Arsenic Resistance and Dissimilatory Reduction
Authors: Uhrynowski Witold , Radlinska Monika , Drewniak Lukasz
Published: February 2019

Shewanella sp. O23S is a dissimilatory arsenate reducing bacterial strain involved in arsenic transformations within the abandoned gold mine in Zloty Stok (SW Poland). Previous physiological studies revealed that O23S may not only release arsenic from minerals, but also facilitate its immobilization through co-precipitation with reduced sulfur species. Given these uncommon, complementary characteristics and the application potential of the strain in arsenic-removal technologies, its genome (~5.3 Mbp), consisting of a single chromosome, two large plasmids (pSheA and pSheB) and three small plasmid-like phages (pSheC-E) was sequenced and annotated. Genes encoding putative proteins involved in heavy metal transformations, antibiotic resistance and other phenotypic traits were identified. An in-depth comparative analysis of arsenic respiration (arr) and resistance (ars) genes and their genetic context was also performed, revealing that pSheB carries the only copy of the arr genes, and a complete ars operon. The plasmid pSheB is therefore a unique natural vector of these genes, providing the host cells arsenic respiration and resistance abilities. The functionality of the identified genes was determined based on the results of the previous and additional physiological studies, including: the assessment of heavy metal and antibiotic resistance under various conditions, adhesion-biofilm formation assay and BiologTM metabolic preferences test. This combined genetic and physiological approach shed a new light on the capabilities of O23S and their molecular basis, and helped to confirm the biosafety of the strain in relation to its application in bioremediation technologies.
EDTA Chelation Therapy for the Treatment of Neurotoxicity
Authors: Fulgenzi Alessandro , Ferrero Maria Elena
Published: February 2019

Neurotoxicity can be caused by numerous direct agents, of which toxic metals, organophosphorus pesticides, air pollution, radiation and electromagnetic fields, neurotoxins, chemotherapeutic and anesthetic drugs, and pathogens are the most important. Other indirect causes of neurotoxicity are cytokine and/or reactive oxygen species production and adoptive immunotherapy. The development of neurodegenerative diseases has been associated with neurotoxicity. Which arms are useful to prevent or eliminate neurotoxicity? The chelating agent calcium disodium ethylenediaminetetraacetic acid (EDTA)—previously used to treat cardiovascular diseases—is known to be useful for the treatment of neurodegenerative diseases. This review describes how EDTA functions as a therapeutic agent for these diseases. Some case studies are reported to confirm our findings.
The HslV Protease from Leishmania major and Its Activation by C-terminal HslU Peptides
Authors: Kebe Ndeye Mathy , Samanta Krishnananda , Singh Priyanka , Lai-Kee-Him Joséphine , Apicella Viviana , Payrot Nadine , Lauraire Noémie , Legrand Baptiste , Lisowski Vincent , Mbang-Benet Diane-Ethna , Pages Michel , Bastien Patrick , Kajava Andrey V. , Bron Patrick , Hernandez Jean-François , Coux Olivier
Published: February 2019

HslVU is an ATP-dependent proteolytic complex present in certain bacteria and in the mitochondrion of some primordial eukaryotes, including deadly parasites such as Leishmania. It is formed by the dodecameric protease HslV and the hexameric ATPase HslU, which binds via the C-terminal end of its subunits to HslV and activates it by a yet unclear allosteric mechanism. We undertook the characterization of HslV from Leishmania major (LmHslV), a trypanosomatid that expresses two isoforms for HslU, LmHslU1 and LmHslU2. Using a novel and sensitive peptide substrate, we found that LmHslV can be activated by peptides derived from the C-termini of both LmHslU1 and LmHslU2. Truncations, Ala- and D-scans of the C-terminal dodecapeptide of LmHslU2 (LmC12-U2) showed that five out of the six C-terminal residues of LmHslU2 are essential for binding to and activating HslV. Peptide cyclisation with a lactam bridge allowed shortening of the peptide without loss of potency. Finally, we found that dodecapeptides derived from HslU of other parasites and bacteria are able to activate LmHslV with similar or even higher efficiency. Importantly, using electron microscopy approaches, we observed that the activation of LmHslV was accompanied by a large conformational remodeling, which represents a yet unidentified layer of control of HslV activation.
Mechanisms of PKC-Mediated Enhancement of HIF-1α Activity and its Inhibition by Vitamin K2 in Hepatocellular Carcinoma Cells
Authors: Xia Jinghe , Ozaki Iwata , Matsuhashi Sachiko , Kuwashiro Takuya , Takahashi Hirokazu , Anzai Keizo , Mizuta Toshihiko
Published: February 2019

Hypoxia-inducible factor 1 (HIF-1) plays important roles in cancer cell biology. HIF-1α is reportedly activated by several factors, including protein kinase C (PKC), in addition to hypoxia. We investigated the role of PKC isoforms and the effects of vitamin K2 (VK2) in the activation process of HIF-1α. Human hepatocellular carcinoma (HCC)-derived Huh7 cells were cultured under normoxic and hypoxic (1% O2) conditions with or without the PKC stimulator TPA. The expression, transcriptional activity and nuclear translocation of HIF-1α were examined under treatment with PKC inhibitors, siRNAs against each PKC isoform and VK2. Hypoxia increased the expression and activity of HIF-1α. TPA increased the HIF-1α activity several times under both normoxic and hypoxic conditions. PKC-δ siRNA-mediated knockdown, PKC-δ inhibitor (rottlerin) and pan-PKC inhibitor (Ro-31-8425) suppressed the expression and transcriptional activity of HIF-1α. VK2 significantly inhibited the TPA-induced HIF-1α transcriptional activity and suppressed the expression and nuclear translocation of HIF-1α induced by TPA without altering the HIF-1α mRNA levels. These data indicate that PKC-δ enhances the HIF-1α transcriptional activity by increasing the nuclear translocation, and that VK2 might suppress the HIF-1α activation through the inhibition of PKC in HCC cells.
Smart Traffic Lights over Vehicular Named Data Networking
Authors: Al-qutwani Majed , Wang Xingwei
Published: February 2019

The existing traffic light system fails to deal with the increase in vehicular traffic requirements due to fixed time programming. Traffic flow suffers from vehicle delay and congestion. A new networking technology called vehicular ad hoc networking (VANET) offers a novel solution for vehicular traffic management. Nowadays, vehicles communicate with each other (V2V), infrastructure (V2I), or roadside units (V2R) using IP-based networks. Nevertheless, IP-based networks demonstrate low performance with moving nodes as they depend on communication with static nodes. Currently, the research community is studying a new networking architecture based on content name called named data networking (NDN) to implement it in VANET. NDN is suitable for VANET as it sends/receives information based on content name, not content address. In this paper, we present one of VANET’s network applications over NDN, a smart traffic light system. Our system solves the traffic congestion issue as well as reducing the waiting time of vehicles in road intersections. This system replaces the current conventional system with virtual traffic lights (VTLs). Instead of installing traffic lights at every intersection, we utilize a road side unit (RSU) to act as the intersection controller. Instead of a light signal, the RSU collects the orders of vehicles that have arrived or will arrive at the intersection. After processing the orders according to the priority policy, the RSU sends an instant message for every vehicle to pass the intersection or wait for a while. The proposed system mimics a human policeman intersection controlling. This approach is suitable for autonomous vehicles as they only receive signals from the RSU instead of processing many images. We provide a map of future work directions for enhancing this solution to take into account pedestrian and parking issues.
Anomaly-Based Method for Detecting Multiple Classes of Network Attacks
Authors: Gurina Anastasia , Eliseev Vladimir
Published: February 2019

The article discusses the problem of detecting network attacks on a web server. The attention is focused on two common types of attacks: “denial of service” and “code injection”. A review and an analysis of various attack detection techniques are conducted. A new lightweight approach to detect attacks as anomalies is proposed. It is based on recognition of the dynamic response of the web server during requests processing. An autoencoder is implemented for dynamic response anomaly recognition. A case study with the MyBB web server is described. Several flood attacks and SQL injection attack are modeled and successfully detected by the proposed method. The efficiency of the detection algorithm is evaluated, and the advantages and disadvantages of the proposed approach are analyzed.
Dinuclear Gold Complexes Supported by Wide Bite Angle Diphosphines for Preorganization-Induced Selective Dual-Gold Catalysis
Authors: Lankelma Marianne , Vreeken Vincent , Siegler Maxime A. , van der Vlugt Jarl Ivar
Published: February 2019

The synthesis, reactivity, and potential of well-defined dinuclear gold complexes as precursors for dual-gold catalysis is explored. Using the preorganizing abilities of well-known wide bite angle diphosphine ligands, DBFPhos and DPEPhos, dinuclear Au(I)–Au(I) complexes 1 and 2 are used as precursors to form well-defined monocationic species with either a chlorido- or acetylido-ligand bridging the two gold centers. These compounds are active catalysts for the dual-gold heterocycloaddition of a urea-functionalized alkyne, and the preorganization of both Au-centers affords efficient σ,π-activation of the substrate, even at high dilution, significantly outperforming benchmark mononuclear catalysts.
Water Oxidation Catalysts: The Quest for New Oxide-Based Materials
Authors: Mavrokefalos Christos K. , Patzke Greta R.
Published: February 2019

The expected shortage of fossil fuels as well as the accompanying climate change are among the major challenges of the 21st century. A global shift to a sustainable energy landscape is, therefore, of utmost importance. Over the past few years, solar technologies have entered the energy market and have paved the way to replace fossil-based energy sources, in the long term. In particular, electrochemical solar-to-hydrogen technologies have attracted a lot of interest—not only in academia, but also in industry. Solar water splitting (artificial photosynthesis) is one of the most active areas in contemporary materials and catalysis research. The development of low-cost, efficient, and stable water oxidation catalysts (WOCs) remains crucial for artificial photosynthesis applications, because WOCs still represent a major economical and efficient bottleneck. In the following, we summarize recent advances in water oxidation catalysts development, with selected examples from 2016 onwards. This condensed survey demonstrates that the ongoing quest for new materials and informed catalyst design is a dynamic and rapidly developing research area.
Fully Ion Implanted Normally-Off GaN DMOSFETs with ALD-Al2O3 Gate Dielectrics
Authors: Yoshino Michitaka , Ando Yuto , Deki Manato , Toyabe Toru , Kuriyama Kazuo , Honda Yoshio , Nishimura Tomoaki , Amano Hiroshi , Kachi Tetsu , Nakamura Tohru
Published: February 2019

A normally-off GaN double-implanted vertical MOSFET (DMOSFET) with an atomic layer deposition (ALD)-Al2O3 gate dielectric film on a free-standing GaN substrate fabricated by triple ion implantation is presented. The DMOSFET was formed with Si ion implanted source regions in a Mg ion implanted p-type base with N ion implanted termination regions. A maximum drain current of 115 mA/mm, maximum transconductance of 19 mS/mm at a drain voltage of 15 V, and a threshold voltage of 3.6 V were obtained for the fabricated DMOSFET with a gate length of 0.4 μm with an estimated p-type base Mg surface concentration of 5 × 1018 cm−3. The difference between calculated and measured Vths could be due to the activation ratio of ion-implanted Mg as well as Fermi level pinning and the interface state density. On-resistance of 9.3 mΩ·cm2 estimated from the linear region was also attained. Blocking voltage at off-state was 213 V. The fully ion implanted GaN DMOSFET is a promising candidate for future high-voltage and high-power applications.
Physicochemical and Biocompatibility Properties of Type I Collagen from the Skin of Nile Tilapia (Oreochromis niloticus) for Biomedical Applications
Authors: Song Wen-Kui , Liu Dan , Sun Lei-Lei , Li Ba-Fang , Hou Hu
Published: February 2019

The aim of this study is to investigate the physicochemical properties, biosafety, and biocompatibility of the collagen extract from the skin of Nile tilapia, and evaluate its use as a potential material for biomedical applications. Two extraction methods were used to obtain acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from tilapia skin. Amino acid composition, FTIR, and SDS-PAGE results showed that ASC and PSC were type I collagen. The molecular form of ASC and PSC is (α1)2α2. The FTIR spectra of ASC and PSC were similar, and the characteristic peaks corresponding to amide A, amide B, amide I, amide II, and amide III were 3323 cm−1, 2931 cm−1, 1677 cm−1, 1546 cm−1, and 1242 cm−1, respectively. Denaturation temperatures (Td) were 36.1 °C and 34.4 °C, respectively. SEM images showed the loose and porous structure of collagen, indicting its physical foundation for use in applications of biomedical materials. Negative results were obtained in an endotoxin test. Proliferation rates of osteoblastic (MC3T3E1) cells and fibroblast (L929) cells from mouse and human umbilical vein endothelial cells (HUVEC) were increased in the collagen-treated group compared with the controls. Furthermore, the acute systemic toxicity test showed no acute systemic toxicity of the ASC and PSC collagen sponges. These findings indicated that the collagen from Nile tilapia skin is highly biocompatible in nature and could be used as a suitable biomedical material.
Endoscopic, Single-Session Management of Encrusted, Forgotten Ureteral Stents
Authors: Ulker Volkan , Celik Orcun
Published: February 2019

Background and Objectives: Remained or forgotten ureteral double-J stents may cause serious complications. Removing of an encrusted, forgotten stent can be challenging. We present our experience with heavily encrusted ureteral stents and discuss the endourologic treatment options as well as their effectivity. Materials and Methods: Eleven men and six women (mean 48.58 ± 14.48 years of age) with 18 encrusted forgotten stents (mean 16.4 ± 13.25 months of indwelling) were treated at our clinic. All patients underwent the operation after negative urine cultures were obtained. Their medical records were retrospectively reviewed and analyzed in terms of number of interventions required to remove the stent, operation time, complications, hospital stay and stone-free rate. Results: According to the Forgotten-Encrusted-Calcified (FECal) classification, the most common form of stent encrustation was grade III (64.7%) and 17.6% of the stents were fragmented. Four of 17 patients were initially treated with extracorporeal shock-wave lithotripsy. The patients required a mean of two endoscopic interventions for removing the encrusted stent and all stents were removed endoscopically in a single session. The mean operating time was 63.3 ± 41.8 minutes. Cystolithotripsy followed by ureteroscopy was the most common intervention (41.1%). Of the 17 patients, peroperative and postoperative complications were Clavien grade I in two, grade II in two and grade IIIb in one. The mean hospital stay was 1.3 ± 0.99 days. All patients were stone-free after a month of stent removal. Conclusions: The endourological removal of the encrusted forgotten stents in a single session is feasible and effective with a minimal morbidity. The treatment strategy should be to minimize the number of interventions.
The Role of the Adenoids in Pediatric Chronic Rhinosinusitis
Authors: Belcher Ryan , Virgin Frank
Published: February 2019

There are several mechanisms by which the adenoids contribute to pediatric chronic rhinosinusitis (PCRS), particularly with children aged 12 years and younger. Understanding the role that the adenoids play in PCRS is crucial when attempting to treat these patients. A literature review was performed to address this problem and provide information surrounding this topic. This review will provide a better understanding of how adenoids contribute to PCRS, and also of the medical and surgical treatment options.
Design of a Micromachined Z-axis Tunneling Magnetoresistive Accelerometer with Electrostatic Force Feedback
Authors: Yang Bo , Wang Binlong , Yan Hongyu , Gao Xiaoyong
Published: February 2019

This paper presents the design, simulation, fabrication and experiments of a micromachined z-axis tunneling magnetoresistive accelerometer with electrostatic force feedback. The tunneling magnetoresistive accelerometer consists of two upper differential tunneling magnetoresistive sensors, a middle plane main structure with permanent magnetic films and lower electrostatic feedback electrodes. A pair of lever-driven differential proof masses in the middle plane main structure is used for sensitiveness to acceleration and closed-loop feedback control. The tunneling magnetoresistive effect with high sensitivity is adopted to measure magnetic field variation caused by input acceleration. The structural mode and mass ratio between inner and outer proof masses are optimized by the Ansys simulation. Simultaneously, the magnetic field characteristic simulation is implemented to analyze the effect of the location of tunneling magnetoresistive sensors, magnetic field intensity, and the dimension of permanent magnetic film on magnetic field sensitivity, which is beneficial for the achievement of maximum sensitivity. The micromachined z-axis tunneling magnetoresistive accelerometer fabricated by the standard deep dry silicon on glass (DDSOG) process has a device dimension of 6400 μm (length) × 6400 μm (width) × 120 μm (height). The experimental results demonstrate the prototype has a maximal sensitivity of 8.85 mV/g along the z-axis sensitive direction under the gap of 1 mm. Simultaneously, Allan variance analysis illustrate that a noise floor of 86.2 μg/Hz0.5 is implemented in the z-axis tunneling magnetoresistive accelerometer.
Nonlinear Vibration of a Micro Piezoelectric Precision Drive System
Authors: Li Chong , Zhong Wei , Fang Jiwen , Sun Lining
Published: February 2019

A micro piezoelectric precision drive system is proposed, which is advantageous due its small size, large transmission ratio, and large output torque. The working principle of the proposed piezoelectric precision drive system is presented, and the nonlinear dynamic model and equations of the system are established. Using the Linz Ted-Poincaré and perturbation methods, the nonlinear approximate solutions of the dynamic equations are calculated. The results indicate that the nonlinear intensity of the drive system is inversely proportional to the number of meshing movable teeth. It was also noted that the rotor is most affected by the nonlinear phenomenon. These results can be utilized both to optimize the dimensions of the piezoelectric precision drive system and to reduce the intensity of vibrations during operation.
High Precision Thermoforming 3D-Conformable Electronics with a Phase-Changing Adhesion Interlayer
Authors: Wu Kang , Zhou Qifeng , Zou Huaping , Leng Kangmin , Zeng Yifan , Wu Zhigang
Published: February 2019

Modern design-conscious products have raised the development of advanced electronic fabricating technologies. These widely used industrial technologies show high compatibility for inorganic materials and capacity for mass production. However, the morphology accuracy is hard to ensure and cracks happen easily, which could cause the degradation of device performance and life span. In order to make high precision 3D conformable electronics, a thermal phase-changing adhesion interlayer and modified fabricating processes are used in self-developed equipment. The working principles and influencing factors such as heating time and geometry parameters are studied quantitatively. The accuracy of fabricated patterns is enhanced by this new technology and serpentine designed structures. The delamination or detachment are significantly alleviated. Due to the operation convenience and compatibility with existing materials, the presented fabrication method has great potential for mass production of 3D curved conformable electronics.
Characterization of Carbonate Crust from a Recently Discovered Methane Seep on the North Atlantic Continental Margin of the USA
Authors: Gabitov Rinat , Borrelli Chiara , Buettner Jacob , Kirkland Brenda , Skarke Adam , Trail Dustin , Garner Brittany , Testa Maurice , Wahidi Mahnaz , Hoff Christopher , Khasanov Salavat , Panieri Giuliana , Thirumalai Roobanvenkatesh , Thomas Jay , Weremeichik Jeremy , Zverkova Irina
Published: February 2019

This study is focused on mineralogical and chemical characterization of an authigenic carbonate rock (crust) collected at a recently discovered cold seep on the US North Atlantic continental margin. X-ray diffraction (XRD) and scanning electron microscopy (SEM) indicate that the carbonate rock is composed of microcrystalline aragonite cement, white acicular aragonite crystals (AcAr), equant quartz crystals, small microcrystalline aluminosilicates, and trace amounts of iron sulfide microcrystals. Element/calcium ratios were measured with laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS) using a calcite standard, which was prepared by annealing USGS certified carbonate powder (MACS-3). The occurrence of microscopic, non-carbonate inclusions precluded evaluation of trace elements in the aragonite cement, but allowed for in situ analysis of AcAr crystals. Carbon and oxygen isotopes were analyzed via isotope ratio mass spectrometry (IRMS) and expressed as δ13C and δ18O. Low δ13C values suggest that aragonite grew as a result of anaerobic oxidation of methane and observed δ18O values indicate that the temperature of aragonite crystallization was 1.7–1.9 °C.
Multivariate Analysis for Assessing Sources, and Potential Risks of Polycyclic Aromatic Hydrocarbons in Lisbon Urban Soils
Authors: Cachada Anabela , Dias Ana Claudia , Reis Amélia Paula , Ferreira da Silva Eduardo , Pereira Ruth , Duarte Armando da Costa , Patinha Carla
Published: February 2019

Urban soils quality may be severely affected by polycyclic aromatic hydrocarbons (PAHs) contamination, as is the case of Lisbon (Portugal). However, to conduct a risk assessment analysis in an urban area can be a very difficult task due to the patchy nature and heterogeneity of these soils. Thus, the present study aims to provide an example on how to perform the first tier of a risk assessment plan in the case of urban soils using a simpler, cost effective, and reliable framework. Thus, a study was conducted in Lisbon to assess the levels of PAH, their potential risks to the environment and human health, and to identify their major sources. Source apportionment was performed by studying PAHs profiles, their relationship with potentially toxic elements, and general characteristics of soil using multivariate statistical methods. Results showed that geostatistical tools are useful for evaluating the spatial distribution and major inputs of PAHs in urban soils, as well as to identify areas of potential concern, showing their usefulness in risk assessment analysis and urban planning. Particularly, the prediction maps obtained allowed for a clear identification of areas with the highest levels of PAHs (close to the airport and in the city center). The high concentrations found in soils from the city center should be a result of long-term accumulation due to diffuse pollution mostly from traffic (through atmospheric emissions, tire debris and fuel exhaust, as well as pavement debris). Indeed, most of the sites sampled in the city center were historical gardens and parks. The calculation of potential risks based on different models showed that there is a high discrepancy among guidelines, and that risks will be extremely associated with the endpoint or parameters used in the different models. Nevertheless, this initial approach based on total levels was useful for identifying areas where a more detailed risk assessment is needed (close to the airport and in the city center). Therefore, the use of prediction maps can be very useful for urban planning, for example, by crossing information obtained with land uses, it is possible to define the most problematic areas (e.g., playgrounds and schools).
Chromo-Fluorogenic Detection of Soman and Its Simulant by Thiourea-Based Rhodamine Probe
Authors: Li Shengsong , Zheng Yongchao , Chen Weiqiang , Zheng Meiling , Zheng He , Zhang Zhe , Cui Yan , Zhong Jinyi , Zhao Chonglin
Published: February 2019

Here, we introduced a novel thiourea-based rhodamine compound as a chromo-fluorogenic indicator of nerve agent Soman and its simulant diethyl chlorophosphate (DCP). The synthesized probe N-(rhodamine B)-lactam-2-(4-cyanophenyl) thiourea (RB-CT), which has a rhodamine core linked by a cyanophenyl thiosemicarbazide group, enabled a rapidly and highly sensitive response to DCP with clear fluorescence and color changes. The detection limit was as low as 2 × 10−6 M. The sensing mechanism showed that opening of the spirolactam ring following the phosphorylation of thiosemicarbazides group formed a seven-membered heterocycle adduct, according to MS analysis and TD-DFT calculations. RB-CT exhibited high detecting selectivity for DCP, among other organophosphorus compounds. Moreover, two test kits were employed and successfully used to detect real nerve agent Soman in liquid and gas phase.
The Inhibition of Radial and Axial Micromovement of Bone Scaffold with Gelfoam® and Titanium Mesh Fixation and Its Effects on Osteointegration
Authors: Kwon Jane , Lee Dong Joon , Kocher Mallory , Kim Yong-Il , Wu Te-Ju , Whitley John , Ko Ching-Chang
Published: February 2019

A major drawback of nanocomposite scaffolds in bone tissue engineering is dimensional shrinkage after the fabrication process. Shrinkage yields gaps between the scaffold and host bone in the defect site and eventually causes failure in osteointegration by micromovement. The present study was conducted using titanium (Ti) mesh and Gelfoam® to prevent radial and axial micromovement, respectively. A critical-sized defect (CSD) was created in the center of the calvarium of Sprague Dawley rats to implant porous polydopamine-laced hydroxyapatite collagen calcium silicate (HCCS-PDA), a novel nanocomposite scaffold. Gelfoam® was applied around the edge of the defect, and then the HCCS-PDA scaffold was inserted in the defect area. Ti mesh was placed between the periosteum and skin right, above the inserted scaffold site. There were two test groups, with a fixture (Gelfoam® and Ti mesh) and without a fixture, each group contained five animals. The rats were sacrificed after three months post-operation. The explanted calvaria underwent micro-CT scanning and a push-out test to quantify osteointegration and mechanical strength between the scaffold and host bone. Histological analysis of undecalcified bone was performed by grinding resin infiltrated calvaria blocks to prepare 10 μm slices. Osteointegration was higher in the group with fixation than without fixation. Movement of the HCCS-PDA scaffold in the gap resulted in diminished osteointegration. With fixation, the movement was inhibited and osteointegration became prominent. Here we present a successful method of preventing axial and radial movement of scaffolds using Gelfoam® and Ti mesh. Applying this fixture, we expect that an HCCS-PDA scaffold can repair CSD more effectively.
A Plant-Based Meal Stimulates Incretin and Insulin Secretion More Than an Energy- and Macronutrient-Matched Standard Meal in Type 2 Diabetes: A Randomized Crossover Study
Authors: Kahleova Hana , Tura Andrea , Klementova Marta , Thieme Lenka , Haluzik Martin , Pavlovicova Renata , Hill Martin , Pelikanova Terezie
Published: February 2019

Diminished postprandial secretion of incretins and insulin represents one of the key pathophysiological mechanisms behind type 2 diabetes (T2D). We tested the effects of two energy- and macronutrient-matched meals: A standard meat (M-meal) and a vegan (V-meal) on postprandial incretin and insulin secretion in participants with T2D. A randomized crossover design was used in 20 participants with T2D. Plasma concentrations of glucose, insulin, C-peptide, glucagon-like peptide-1 (GLP-1), amylin, and gastric inhibitory peptide (GIP) were determined at 0, 30, 60, 120, and 180 min. Beta-cell function was assessed with a mathematical model, using C-peptide deconvolution. Repeated-measures ANOVA was used for statistical analysis. Postprandial plasma glucose responses were similar after both test meals (p = 0.64). An increase in the stimulated secretion of insulin (by 30.5%; 95% CI 21.2 to 40.7%; p < 0.001), C-peptide (by 7.1%; 95% CI 4.1 to 9.9%; p < 0.001), and amylin (by 15.7%; 95% CI 11.8 to 19.7%; p < 0.001) was observed following consumption of the V-meal. An increase in stimulated secretion of GLP-1 (by 19.2%; 95% CI 12.4 to 26.7%; p < 0.001) and a decrease in GIP (by −9.4%; 95% CI −17.3 to −0.7%; p = 0.02) were observed after the V-meal. Several parameters of beta-cell function increased after the V-meal, particularly insulin secretion at a fixed glucose value 5 mmol/L, rate sensitivity, and the potentiation factor. Our results showed an increase in postprandial incretin and insulin secretion, after consumption of a V-meal, suggesting a therapeutic potential of plant-based meals for improving beta-cell function in T2D.
Gluten Free Wheat: Are We There?
Authors: García-Molina María Dolores , Giménez María José , Sánchez-León Susana , Barro Francisco
Published: February 2019

Gluten proteins, major determinants of the bread-making quality of wheat, are related to several digestive disorders. Advances in plant genetic breeding have allowed the production of wheat lines with very low gliadin content through the use of RNAi and gene editing technologies. In this review, we carried out a comprehensive study of the application of these cutting-edge technologies towards the development of wheat lines devoid of immunogenic gluten, and their genetic, nutritional and clinical characterization. One line, named E82, showed outstanding nutritional properties, with very low immunogenic gluten and a low stimulation capacity of T-cells from celiac patients. Moreover, a clinical trial with non-celiac wheat sensitivity (NCWS) patients showed that the consumption of bread made with this E82 low gliadin line induced positive changes in the gut microbiota composition.
Agreement among Mediterranean Diet Pattern Adherence Indexes: MCC-Spain Study
Authors: Olmedo-Requena Rocío , González-Donquiles Carmen , Dávila-Batista Verónica , Romaguera Dora , Castelló Adela , Molina de la Torre Antonio José , Amiano Pilar , Dierssen-Sotos Trinidad , Guevara Marcela , Fernández-Tardón Guillermo , Lozano-Lorca Macarena , Alguacil Juan , Peiró Rosana , Huerta José María , Gracia-Lavedan Esther , Aragonés Nuria , Fernández-Villa Tania , Solans Marta , Gómez-Acebo Inés , Castaño-Vinyals Gemma , Kogevinas Manolis , Pollán Marina , Martín Vicente
Published: February 2019

There are many different methods used to measure the degree of adherence to a Mediterranean diet (MD), limiting comparison and interpretation of their results. The concordance between different methodologies has been questioned and their evaluation recommended. The aim of this study was to evaluate the agreement among five indexes that measure adherence to a Mediterranean dietary pattern. The study population included healthy adults selected in the Multi-Case Control Spain (MCC-Spain) study recruited in 12 provinces. A total of 3640 controls were matched to cases by age and sex. To reach the aim, the following scores of adherence to a Mediterranean dietary pattern were calculated: Mediterranean diet score (MDS), alternative Mediterranean diet (aMED), relative Mediterranean diet (rMED), dietary score (DS) and literature-based adherence score (LBAS). The relative frequency of subjects with a high level of adherence to a MD varied from 22% (aMED index) to 37.2% (DS index). Similarly, a high variability was observed for the prevalence of a low level of MD: from 24% (rMED) to 38.4% (aMED). The correlation among MDS, aMED and rMED indexes was moderate, except for MDS and aMED with a high coefficient of correlation 0.75 (95% CI 0.74–0.77). The Cohen’s Kappa coefficient among indexes showed a moderate–fair concordance, except for MDS and aMED with a 0.56 (95% CI 0.55–0.59) and 0.67 (95% CI 0.66–0.68) using linear and quadratic weighting, respectively. The existing MD adherence indexes measured the same, although they were based on different constructing algorithms and varied in the food groups included, leading to a different classification of subjects. Therefore, concordance between these indexes was moderate or low.
The Differences in Postprandial Serum Concentrations of Peptides That Regulate Satiety/Hunger and Metabolism after Various Meal Intake, in Men with Normal vs. Excessive BMI
Authors: Adamska-Patruno Edyta , Ostrowska Lucyna , Goscik Joanna , Fiedorczuk Joanna , Moroz Monika , Kretowski Adam , Gorska Maria
Published: February 2019

The energy balance regulation may differ in lean and obese people. The purposes of our study were to evaluate the hormonal response to meals with varying macronutrient content, and the differences depending on body weight. Methods. The crossover study included 46 men, 21–58 years old, normal-weight and overweight/obese. Every subject participated in two meal-challenge-tests with high-carbohydrate (HC), and normo-carbohydrate (NC) or high-fat (HF) meals. Fasting and postprandial blood was collected for a further 240 min, to determine adiponectin, leptin and total ghrelin concentrations. Results. In normal-weight individuals after HC-meal we observed at 60min higher adiponectin concentrations (12,554 ± 1531 vs. 8691 ± 1070 ng/mL, p = 0.01) and significantly (p < 0.05) lower total ghrelin concentrations during the first 120 min, than after HF-meal intake. Fasting and postprandial leptin levels were significantly (p < 0.05) higher in overweigh/obese men. Leptin concentrations in normal-weight men were higher (2.72 ± 0.8 vs. 1.56 ± 0.4 ng/mL, p = 0.01) 180 min after HC-meal than after NC-meal intake. Conclusions. Our results suggest that in normal-body weight men we can expect more beneficial leptin, adiponectin, and total ghrelin response after HC-meal intake, whereas, in overweight/obese men, the HC-meal intake may exacerbate the feeling of hunger, and satiety may be induced more by meals with lower carbohydrate content.