bluuebunny/crossref_metadata_2025 huggingface dataset:
The effects and mechanisms of natural products on Helicobacter pylori eradication
Authors: Deng Ruiyi , Chen Xu , Zhao Shiqing , Zhang Qingying , Shi Yanyan
Published: February 2024

Helicobacter pylori (H. pylori) eradication is pivotal for alleviating gastric mucosal inflammation and preventing the progression of gastric diseases. While antibiotic-based therapies have achieved significant success in H. pylori eradication, challenges such as antibiotic resistance, drug toxicity, side effects, nonadherence, inapplicability, and disruption of gastrointestinal microflora have emerged. Updated therapies are urgently needed to suppress H. pylori. Nature has provided multitudinous therapeutic agents since ancient times. Natural products can be a potential therapy endowed with H. pylori eradication efficacy. We summarize the basic information, possible mechanisms, and the latest research progress of some representative natural products in H. pylori eradication, highlighting their safety, accessibility, efficiency, and ability to overcome limitations associated with antibiotic application. This review highlights the potential therapeutic advantages of incorporating ethnomedicine into anti-H. pylori regimens. The findings of this review may provide insights into the development of novel natural products and expand the therapeutic options available for H. pylori eradication.
Hypoxia-inducible factor-2α promotes fibrosis in non-alcoholic fatty liver disease by enhancing glutamine catabolism and inhibiting yes-associated protein phosphorylation in hepatic stellate cells
Authors: Yan Ranran , Cai Hao , Zhou Xiaofeng , Bao Guodan , Bai Zhenzhong , Ge Ri-li
Published: February 2024

Non-alcoholic fatty liver disease (NAFLD) has a high global prevalence and affects approximately one-third of adults, owing to high-fat dietary habits and a sedentary lifestyle. The role of hypoxia-inducible factor 2α (HIF-2α) in NAFLD progression remains unknown. This study aimed to investigate the effects of chronic hypoxia on NAFLD progression by examining the role of hypoxia-inducible factor 2α (HIF-2α) activation and that of hepatic stellate cell (HSC)-derived myofibroblasts through glutaminolysis. We hypothesised that hypoxia exacerbates NAFLD by promoting HIF-2α upregulation and inhibiting phosphorylated yes-associated protein (YAP), and that increasing YAP expression enhances HSC-derived myofibroblasts. We studied patients with NAFLD living at high altitudes, as well as animal models and cultured cells. The results revealed significant increases in HSC-derived myofibroblasts and collagen accumulation caused by HIF-2α and YAP upregulation, both in patients and in a mouse model for hypoxia and NAFLD. HIF-2α and HIF-2α-dependent YAP downregulation reduced HSC activation and myofibroblast levels in persistent chronic hypoxia. Furthermore, hypoxia-induced HIF-2α upregulation promoted YAP and inhibited YAP phosphorylation, leading to glutaminase 1 (GLS1), SLC38A1, α-SMA, and Collagen-1 overexpression. Additionally, hypoxia restored mitochondrial adenosine triphosphate production and reactive oxygen species (ROS) overproduction. Thus, chronic hypoxia-induced HIF-2α activation enhances fibrosis and NAFLD progression by restoring mitochondrial ROS production and glutaminase-1-induced glutaminolysis, which is mediated through the inhibition of YAP phosphorylation and increased YAP nuclear translocation. In summary, HIF-2α plays a pivotal role in NAFLD progression during chronic hypoxia.
Incident comorbidities in patients with chronic hypoparathyroidism after thyroidectomy: a multicenter nationwide study
Authors: Díez Juan J. , Anda Emma , Pérez-Corral Begoña , Paja Miguel , Alcázar Victoria , Sánchez-Ragnarsson Cecilia , Orois Aida , Romero-Lluch Ana R. , Sambo Marcel , Oleaga Amelia , Caballero Águeda , Alhambra María R. , Urquijo Virginia , Delgado-Lucio Ana M. , Fernández-García José C. , Kishore-Doulatram Viyey , Dueñas-Disotuar Suset , Martín Tomás , Peinado Mercedes , Sastre Julia
Published: February 2024

Purpose

Population-based and registry studies have shown that chronic hypoparathyroidism is accompanied by long-term complications. We aimed to evaluate the risk of incident comorbidity among patients with chronic postsurgical hypoparathyroidism in real-life clinical practice in Spain.

Methods

We performed a multicenter, retrospective cohort study including patients with chronic postsurgical hypoparathyroidism lasting ≥3 years with at least a follow-up visit between January 1, 2022 and September 15, 2023 (group H). The prevalence and incidence of chronic complications including chronic kidney disease, nephrolithiasis/nephrocalcinosis, hypertension, dyslipidemia, diabetes, cardiovascular disease, central nervous system disease, mental health disorders, eye disorders, bone mineral density alterations, fracture and cancer were evaluated. Patient data were compared with a group of patients who did not develop hypoparathyroidism, matched by gender, age, and follow-up time after thyroidectomy (group NH).

Results

We included 337 patients in group H (median [IQR] age, 45 [36-56] years; median time of follow-up, 8.9 [6.0-13.0] years; women, 84.3%) and 669 in group NH (median age, 47 [37-55] years; median time of follow-up, 8.0 [5.3-12.0] years; women, 84.9%). No significant differences were found in the prevalence of comorbidities at the time of thyroidectomy between both groups. In multivariable adjusted analysis, patients with chronic hypoparathyroidism had significantly higher risk of incident chronic kidney disease (OR, 3.45; 95% CI, 1.72-6.91; P<0.001), nephrolithiasis (OR, 3.34; 95% CI, 1.55-7.22; P=0.002), and cardiovascular disease (OR, 2.03; 95% CI, 1.14-3.60; P=0.016), compared with patients without hypoparathyroidism. On the contrary, the risk of fracture was decreased in patients with hypoparathyroidism (OR, 0.09; 95% CI, 0.01-0.70; P=0.021).

Conclusion

This study demonstrates that, in the clinical practice of Spanish endocrinologists, a significant increase in the risk of chronic kidney disease, nephrolithiasis and cardiovascular disease, as well as a reduction in the risk of fractures is detected. These results are of interest for the development of new clinical guidelines and monitoring protocols for patients with hypoparathyroidism.
A bi-layer optimization method of the grid-connected microgrid based on the multi-strategy of the beluga whale algorithm
Authors: Zhong Xianjing , Sun Xianbo , Wu Yuhan
Published: February 2024

Endeavoring to enhance the penetration rate of renewable energy sources, concurrently ensuring economic and operational stability, this study proposes a novel bi-layer optimization method of the wind–solar-storage AC/DC microgrid (MG). First, by incorporating a superordinate electric and seasonal hydrogen hybrid energy storage system (E&SHESS), the topology structure of the microgrid is established. Subsequently, to rectify the intrinsic limitations of the conventional beluga whale optimization (BWO) algorithm, this paper proposes a multi-strategy hybrid improvement to BWO (MHIBWO). This innovative improvement integrates an MTent strategy, a step size adjustment mechanism, and a crisscross strategy. Then, constructing a bi-layer iterative model based on the topology, annual net income and grid-connected friendliness are introduced as optimization objectives for the outer and inner layers, respectively, utilizing MHIBWO and CPLEX for resolution. Through a nested iteration of the two layers, the model outputs the capacity scheme with the best performance of economy and stability. Finally, the simulation unequivocally demonstrated the superiority of MHIBWO and the model proposed. In addition, based on the real data of the Elia power station, the validity of the method in operation is tested using the fuzzy C-means algorithm (FCMA) to extract and aggregate typical days, thereby presenting a sophisticated solution for the field of microgrids optimization configuration.
Short-term optimization scheduling method of cascade hydropower and photovoltaic complementary system based on pumping station operation strategy
Authors: Hanxuan Liu , Linjun Shi , Hao Fu , Feng Wu
Published: February 2024

With the rapid development of photovoltaic power generation, how to improve the photovoltaic grid connection rate is an urgent problem to be solved. This article proposes an optimized scheduling method for the water and photovoltaic complementary system, taking into account the operation strategy of pump stations to improve the photovoltaic grid connection rate. Firstly, a multi-objective optimization scheduling model is constructed to consider both power generation and output fluctuation, and the uncertainty of photovoltaic power generation is analyzed from multiple perspectives. Then, taking the cascade hydropower stations and surrounding photovoltaic power stations in a river basin in Sichuan as an example, the operation strategy of pump stations is introduced into the water and photovoltaic complementary system, considering different weather scenarios, to reduce the photovoltaic curtailment rate. The study verifies that the introduction of pump stations can effectively increase the photovoltaic grid connection rate, and quantitatively analyzes the pump station capacity configuration under different photovoltaic penetration rates.
Driving sustainable competitiveness: unveiling the nexus of green intellectual capital and environmental regulations on greening SME performance
Authors: Sohu Jan Muhammad , Hongyun Tian , Junejo Ikramuddin , Akhtar Sadaf , Ejaz Faisal , Dunay Anna , Hossain Md Billal
Published: February 2024

Within the evolving landscape of contemporary business, where sustainability and innovation intertwine, our study explores a strategic gap, presenting the rationale behind choosing green intellectual capital (GIC), absorptive capacity (AC), green innovation (GI), environmental regulations (ERs), and competitive advantage (CA) as main constructs. This model investigates the complex nature of the competitive landscape of SMEs within the vibrant manufacturing sector using the latest version of SmartPLS 4 for analyzing complex data. Drawing upon the theory of the natural resource-based view (NRBV), this study uncovers that GIC advances GI and AC, driving SMEs toward sustainable CA, particularly within the context of ER. These findings propose vital insights for SMEs looking to leverage their valuable resource, contributing to greening operations and reaching sustainable competitiveness while also contributing to theoretical developments in understanding resource-based perceptions on greening the manufacturing of SMEs. Findings reveal the significant impact of GIC on AC and GI and their subsequent impact on CA. Furthermore, ER uncovers the essential role in shaping the link between GIC and AC, thus enhancing SMEs’ capacity to achieve sustainable competitiveness. This study discloses how SMEs utilize GIC to identify ER and achieve CA and how the NRBV develops an understanding of SMEs utilizing their unique resource endowments to drive GI and achieve CA. This study examines the mediating role of AC and GI in this relationship.
Perceptions of wildlife in rehabilitation from images and statements
Authors: Buckanoff Halley , Williams Kai
Published: February 2024

People form perceptions of the approachability of wild animals from images and statements pertaining to non-domestic animals under human care. The exact perceptions have been previously studied in primates. A survey using constructed images and statements about wild animals in care was circulated via email and social media to stakeholders in wildlife rehabilitation. Respondents were asked to categorize each statement or image as representing a pet, education animal, or a releasable wild animal. The results, specifically the finding that 94% of the respondents chose wild animal when presented with a best-practice image, supported our hypothesis that best-practice images and technical names provoked perceptions that the animals were releasable wild animals. Contrary to our hypothesis that not-recommended practices and anthropomorphic labels would lead to perceptions of the animals as pets, we instead found that these scenarios produced an ambiguous grouping of the categories by the respondents. When presented with an ambiguous image, 48% of the respondents labeled it as a pet and 42% labeled it as a wild animal. Practitioners of wildlife rehabilitation should always use clear technical language and employ best-practice images in their public communications to appropriately portray the animals as wild denizens temporarily in care.
Enhancing multi-objective evolutionary algorithms with machine learning for scheduling problems: recent advances and survey
Authors: Zhang Wenqiang , Xiao Guanwei , Gen Mitsuo , Geng Huili , Wang Xiaomeng , Deng Miaolei , Zhang Guohui
Published: February 2024

Multi-objective scheduling problems in workshops are commonly encountered challenges in the increasingly competitive market economy. These scheduling problems require a trade-off among multiple objectives such as time, energy consumption, and product quality. The importance of each optimization objective typically varies in different time periods or contexts, necessitating decision-makers to devise optimal scheduling plans accordingly. In actual production, decision-makers confront intricate multi-objective scheduling problems that demand balancing clients’ requirements and corporate interests while concurrently striving to reduce production cycles and costs. In solving various problems, multi-objective evolutionary algorithms have attracted the attention of researchers and gradually become one of the mainstream methods to solve these problems. In recent years, research combining multi-objective evolutionary algorithms with machine learning technology has shown great potential, opening up new prospects for improving the performance of multi-objective evolutionary methods. This article comprehensively reviews the latest application progress of machine learning in multi-objective evolutionary algorithms for scheduling problems. We review various machine learning techniques employed for enhancing multi-objective evolutionary algorithms, particularly focusing on different types of reinforcement learning methods. Different categories of scheduling problems addressed using these methods were also discussed, including flow-shop scheduling issues, job-shop scheduling challenges, and more. Finally, we highlighted the challenges faced by the field and outlined future research directions.
IL-6 signaling drives self-renewal and alternative activation of adipose tissue macrophages
Authors: Ackermann Jan , Arndt Lilli , Fröba Janine , Lindhorst Andreas , Glaß Markus , Kirstein Michaela , Hobusch Constance , Wunderlich F. Thomas , Braune Julia , Gericke Martin
Published: February 2024

Introduction

Obesity is associated with chronic low-grade inflammation of adipose tissue (AT) and an increase of AT macrophages (ATMs) that is linked to the onset of type 2 diabetes. We have recently shown that neutralization of interleukin (IL)-6 in obese AT organ cultures inhibits proliferation of ATMs, which occurs preferentially in alternatively activated macrophage phenotype.

Methods

In this study, we investigated AT biology and the metabolic phenotype of mice with myeloid cell-specific IL-6Rα deficiency (Il6raΔmyel) after normal chow and 20 weeks of high-fat diet focusing on AT inflammation, ATM polarization and proliferation. Using organotypical AT culture and bone marrow derived macrophages (BMDMs) of IL-4Rα knockout mice (Il4ra-/-) we studied IL-6 signaling.

Results

Obese Il6raΔmyel mice exhibited no differences in insulin sensitivity or histological markers of AT inflammation. Notably, we found a reduction of ATMs expressing the mannose receptor 1 (CD206), as well as a decrease of the proliferation marker Ki67 in ATMs of Il6raΔmyel mice. Importantly, organotypical AT culture and BMDM data of Il4ra-/- mice revealed that IL-6 mediates a shift towards the M2 phenotype independent from the IL-6/IL-4Rα axis.

Discussion

Our results demonstrate IL-4Rα-independent anti-inflammatory effects of IL-6 on macrophages and the ability of IL-6 to maintain proliferation rates in obese AT.
Metabolic changes in fibroblast-like synoviocytes in rheumatoid arthritis: state of the art review
Authors: Hu Zhipeng , Li Yuan , Zhang Lili , Jiang Yayi , Long Caiyi , Yang Qiyue , Yang Maoyi
Published: February 2024

Fibroblast-like synoviocytes (FLS) are important components of the synovial membrane. They can contribute to joint damage through crosstalk with inflammatory cells and direct actions on tissue damage pathways in rheumatoid arthritis (RA). Recent evidence suggests that, compared with FLS in normal synovial tissue, FLS in RA synovial tissue exhibits significant differences in metabolism. Recent metabolomic studies have demonstrated that metabolic changes, including those in glucose, lipid, and amino acid metabolism, exist before synovitis onset. These changes may be a result of increased biosynthesis and energy requirements during the early phases of the disease. Activated T cells and some cytokines contribute to the conversion of FLS into cells with metabolic abnormalities and pro-inflammatory phenotypes. This conversion may be one of the potential mechanisms behind altered FLS metabolism. Targeting metabolism can inhibit FLS proliferation, providing relief to patients with RA. In this review, we aimed to summarize the evidence of metabolic changes in FLS in RA, analyze the mechanisms of these metabolic alterations, and assess their effect on RA phenotype. Finally, we aimed to summarize the advances and challenges faced in targeting FLS metabolism as a promising therapeutic strategy for RA in the future.
The impact of photodynamic therapy on immune system in cancer – an update
Authors: Dudzik Tomasz , Domański Igor , Makuch Sebastian
Published: February 2024

Photodynamic therapy (PDT) is a therapeutic approach that has gained significant attention in recent years with its promising impact on the immune system. Recent studies have shown that PDT can modulate both the innate and adaptive arms of the immune system. Currently, numerous clinical trials are underway to investigate the effectiveness of this method in treating various types of cancer, as well as to evaluate the impact of PDT on immune system in cancer treatment. Notably, clinical studies have demonstrated the recruitment and activation of immune cells, including neutrophils, macrophages, and dendritic cells, at the treatment site following PDT. Moreover, combination approaches involving PDT and immunotherapy have also been explored in clinical trials. Despite significant advancements in its technological and clinical development, further studies are needed to fully uncover the mechanisms underlying immune activation by PDT. The main objective of this review is to comprehensively summarize and discuss both ongoing and completed studies that evaluate the impact of PDT of cancer on immune response.
Systemic immune-inflammatory biomarkers (SII, NLR, PLR and LMR) linked to non-alcoholic fatty liver disease risk
Authors: Liu Ke , Tang Shiyun , Liu Chenhao , Ma Jianli , Cao Xiyu , Yang Xiuli , Zhu Yi , Chen Ke , Liu Ya , Zhang Chuantao , Liu Yi
Published: February 2024

Background

Systemic immune-inflammatory biomarkers including systemic immune inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-to-monocyte ratio (LMR) have been demonstrated to be associated with the risk and severity of various liver diseases. However, studies on their role and clinical significance in metabolic diseases, especially in nonalcoholic fatty liver disease (NAFLD), are limited and results are inconsistent.

Methods

10821 adults aged 20 years or older were enrolled in this cross-sectional study, sourced from six cycles of the National Health and Nutrition Examination Survey (NHANES). Survey-weighted logistic regression was employed to investigate the correlation between systemic immune-inflammatory biomarkers (SII, NLR, PLR, and LMR) and NAFLD risk. Restricted cubic spline regression models and segmented regression models were used to describe nonlinear relationships and threshold effects. Subgroup and sensitivity analyses were also conducted.

Results

After adjusting for all confounding variables, there was a significant positive association observed between ln-transformed SII (OR= 1.46, 95% CI: 1.27-1.69, P <0.001), NLR (OR= 1.25, 95% CI: 1.05-1.49, P =0.015), LMR (OR= 1.39, 95% CI: 1.14-1.69, P = 0.002) with NAFLD. A nonlinear dose-response relationship with an inverted “U”-shaped threshold of 4.64 was observed between ln(PLR) and NAFLD risk. When ln(PLR) was below 4.64, each unit increase in ln(PLR) was associated with a 0.55-fold increase in the risk of NAFLD (OR= 1.55, 95% CI: 1.05-2.31, P <0.05). Conversely, when ln(PLR) exceeded 4.64, each unit increase in ln(PLR) was associated with a 0.40-fold decrease in the risk of NAFLD (OR= 0.60, 95% CI. 0.44-0.81, P <0.05).

Conclusion

ln-transformed SII, NLR, and LMR were linearly associated with NAFLD risk. ln(PLR) showed an inverted “U”-shaped nonlinear dose-response relationship with the risk of NAFLD.
Sensorineural hearing loss and cognitive impairment: three hypotheses
Authors: Zhao He , Wang Yan , Cui Limei , Wang Hanjing , Liu Sha , Liang Tiantian , Liu Dawei , Qiu Jingjing , Chen Liang , Sun Yan
Published: February 2024

Sensorineural hearing loss (SNHL) is a category of hearing loss that often leads to difficulty in understanding speech and other sounds. Auditory system dysfunction, including deafness and auditory trauma, results in cognitive deficits via neuroplasticity. Cognitive impairment (CI) refers to an abnormality in the brain’s higher intellectual processes related to learning, memory, thinking and judgment that can lead to severe learning and memory deficits. Studies have established a strong correlation between SNHL and CI, but it remains unclear how SNHL contributes to CI. The purpose of this article is to describe three hypotheses regarding this relationship, the mainstream cognitive load hypothesis, the co-morbidity hypothesis, and the sensory deprivation hypothesis, as well as the latest research progress related to each hypothesis.
Stroke-associated pneumonia according to mCDC criteria: impact on prognosis and antibiotic therapy
Authors: Rabaneda-Lombarte Neus , Faura Júlia , Ezcurra-Díaz Garbiñe , Olivé-Gadea Marta , Álvarez-Larruy Marta , Vidal-de Francisco Diana , Domínguez-Mayoral Ana , Avellaneda Carla , Freijo Mari Mar , Zapata-Arriaza Elena , Serrano-Heras Gemma , Alcahut-Rodríguez Cristian , Fernández-Pérez Isabel , Moniche Francisco , Pérez-Sánchez Soledad , Millán Mònica , Rubiera Marta , Dorado Laura , Maisterra Olga , Montaner Joan , Bustamante Alejandro
Published: February 2024

Objective

The modified Centers for Disease Control and Prevention (mCDC) criteria have been proposed for diagnosing and managing stroke-associated pneumonia (SAP). The objective was to investigate the impact of SAP on stroke outcome depending on whether or not it conforms to mCDC criteria. Our secondary objective was to identify the responsible factors for antibiotic initiation in stroke patients.

Methods

We conducted a prospective, multicenter, observational study of ischemic stroke patients with moderate to severe stroke (NIHSS≥4) admitted within 24 h. For 7 days, mCDC criteria were assessed daily, and infections and antibiotics were recorded. Pneumonias were divided into those fulfilling mCDC criteria (mCDC-SAP) or not (other pneumonias, OPn). The effect of each type of pneumonia on 3-month outcome was evaluated in separated logistic regression models. Factors associated with antibiotic initiation were explored using a random forest analysis.

Results

Of the 342 patients studied, infections were diagnosed in 72 (21.6%), including 39 (11.7%) cases of pneumonia. Of them, 25 (7.5%) fulfilled mCDC criteria. Antibiotics were used in 92% of mCDC-SAP and 64.3% of OPn. In logistic regression analysis, mCDC-SAP, but not OPn, was an independent predictor of poor outcome [OR, 4.939 (1.022–23.868)]. The random forest analysis revealed that fever had the highest importance for antibiotic initiation.

Interpretation

The mCDC criteria might be useful for detecting clinically relevant SAP, which is associated with poor outcomes. Isolated signs of infection were more important for antibiotic initiation than compliance with pre-defined criteria. Therefore, adherence to mCDC criteria might result in antibiotic saving without compromising clinical outcome.
Prediction of pulmonary infection in patients with severe myelitis by NPAR combined with spinal cord lesion segments
Authors: Yang Fan , Dong Ruirui , Wang Yating , Guo Junshuang , Zang Qiuling , Wen Lijun , Huang Peipei , Qin Jinjin , Song Dandan , Ren Zhiping , Teng Junfang , Miao Wang
Published: February 2024

Objectives

To investigate the risk factors of pulmonary infection in patients with severe myelitis and construct a prediction model.

Methods

The clinical data of 177 patients with severe myelitis at admission from the First Affiliated Hospital of Zhengzhou University from January 2020 to December 2022 were retrospectively analyzed. The predicting factors associated with pulmonary infection were screened by multivariate logistic regression analysis, and the nomogram model was constructed, and the predictive efficiency of the model was evaluated, which was verified by calibration curve, Hosmer–Lemeshow goodness-of-fit test and decision curve analysis.

Results

Of the 177 patients with severe myelitis, 38 (21.5%) had pulmonary infection. Multivariate logistic regression analysis showed that neutrophil percentage to albumin ratio (NPAR) (OR = 6.865, 95%CI:1.746–26.993, p = 0.006) and high cervical cord lesion (OR = 2.788, 95%CI:1.229–6.323, p = 0.014) were independent risk factors for pulmonary infection, and the combined nomogram could easily predict the occurrence of pulmonary infection, with a C-index of 0.766 (95% CI: 0.678–0.854). The calibration curve, Hosmer-Lemeshow goodness-of-fit test (χ2 = 9.539, p = 0.299) and decision curve analysis showed that the model had good consistency and clinical applicability.

Conclusion

The nomogram model constructed based on NPAR combined with high cervical cord lesion at admission has good clinical application value in predicting pulmonary infection in patients with severe myelitis, which is conducive to clinicians’ evaluation of patients.
Binocular function in the aging visual system: fusion, suppression, and stereoacuity
Authors: Song Yutong , Wang Xi , Liao Meng , Baldwin Alex S. , Liu Longqian
Published: February 2024

Introduction

Changes in vision that occur in normal healthy aging can be seen in fundamental measures of monocular vision. However, the nature of the changes in binocular vision with age remain unclear.

Methods

A total of 28 older (53–66 years) and 28 younger adults (20–31 years) were enrolled in this study. We performed a battery of tests to assess differences in monocular contrast thresholds and various binocular visual functions including dichoptic masking weight and strength, the binocular balance point for fused stimuli, and stereoacuity in the aging and control groups.

Results

Aging significantly increased monocular contrast thresholds (p < 0.001). Although this suggests that aging reduces the effective “input gain” to vision, we also found a significantly elevated contribution of those weaker signals to interocular suppression (p < 0.001). Consequently, there was no significant net difference in the strength of interocular suppression (p = 0.065). We did not find a significant difference of absolute balance point between the two groups (p = 0.090). Lastly, the mean stereoacuity was worse in the older group compared to the younger group (p = 0.002).

Discussion

Our findings confirm previous results showing differences in contrast sensitivity and stereoacuity with aging. Furthermore, we find a change in interocular suppression that is a possible consequence of the change in contrast sensitivity. It is suggestive of a cortical system that maintains a homeostatic balance in interocular suppression across the lifespan.
Optical measurement of glutamate release robustly reports short-term plasticity at a fast central synapse
Authors: Hain Paul Jakob Habakuk , Moser Tobias
Published: February 2024

Introduction

Recently developed fluorescent neurotransmitter indicators have enabled direct measurements of neurotransmitter in the synaptic cleft. Precise optical measurements of neurotransmitter release may be used to make inferences about presynaptic function independent of electrophysiological measurements.

Methods

Here, we express iGluSnFR, a genetically encoded glutamate reporter in mouse spiral ganglion neurons to compare electrophysiological and optical readouts of presynaptic function and short-term synaptic plasticity at the endbulb of Held synapse.

Results

We show iGluSnFR robustly and approximately linearly reports glutamate release from the endbulb of Held during synaptic transmission and allows assessment of short-term plasticity during high-frequency train stimuli. Furthermore, we show that iGluSnFR expression slightly alters the time course of spontaneous postsynaptic currents, but is unlikely to impact measurements of evoked synchronous release of many synaptic vesicles.

Discussion

We conclude that monitoring glutamate with optical sensors at fast and large central synapses like the endbulb of Held is feasible and allows robust quantification of some, but not all aspects of glutamate release.
Simultaneous occurrence of extralobar pulmonary sequestration, esophageal duplication, and bronchogenic cysts in a Chinese child: a rare case report
Authors: Zhao Huashan , Zhai Yunpeng , Guo Rui , Xu Hongxiu , Huang Sai , Lv Longfei , Zhang Shisong
Published: February 2024

The occurrence of simultaneous extralobar pulmonary sequestration, esophageal duplication, and bronchogenic cysts is relatively low. We report the case of a 9-month-old Chinese child who had a right lung cyst, detected in utero and was closely monitored until birth. At age 9 months, contrast-enhanced computed tomography revealed right mediastinal extralobar pulmonary sequestration and two cysts. The patient did not exhibit any abnormalities. However, the parents were concerned about the disease. Following positive psychological counseling to the parents, surgery was the strong desire. Subsequently, successful thoracoscopic surgery was performed, excising the three lesions. No postoperative complications occurred. Postoperative pathology confirmed extralobar pulmonary sequestration syndrome combined with esophageal duplication and bronchogenic cysts. The patient was followed-up at 1 and 12 months postoperatively and recovered well with no abnormal space occupation. In such cases, preoperative imaging examinations should be carefully performed, and intraoperative exploration should correspond to that before surgery to avoid lesion omission.
Chiral phthalimides against penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus: molecular docking and in vitro analysis
Authors: Khan Aamina Azam , Khan Momin , Khan Sher Wali , Siddique Nayyer , Abid Rimsha , Zulfiqar Shandana , Rahman Sidra , Ali Muhammad
Published: February 2024

Staphylococcus aureus (S. aureus) is a commensal bacterium and an opportunistic pathogen causing a wide variety of infections ranging from localized skin and soft tissue infections to life-threatening severe bacteremia, osteomyelitis, endocarditis, atopic dermatitis, prosthetic joint infection, staphylococcal food poisoning, medical device-related infections, and pneumonia. It is attributed to an acquired resistant gene, mecA, encoding penicillin-binding protein 2a (PBP2a). PBP2a is an essential protein responsible for the resistivity of methicillin-resistant S. aureus (MRSA) to various beta-lactam antibiotics. The antimicrobial treatment alternatives for MRSA are increasingly limited. Therefore, developing alternative therapeutic options for its treatment is the need of the day. Phthalimides and their N-substituted derivatives are of biological importance as they possess extensive biological and pharmaceutical properties and can serve as an excellent therapeutic option for MRSA. This study uses three chiral phthalimides (FIA, FIB, and FIC) to check their in silico and in vitro inhibitory effects. Molecular docking of these chiral phthalimides against PBP2a of MRSA was performed initially. After promising results, these novel compounds were screened through the agar-well diffusion method and micro-broth dilution assay to investigate their in vitro inhibitory activities with FIB being the strongest anti-staphylococcal agent yielding a 21 mm zone of inhibition and a minimum inhibitory concentration (MIC) of 0.022 ug, respectively. The zones of inhibition obtained through the in vitro activity showed that these chiral phthalimides possess substantial anti-MRSA activities and have the potential to be considered as alternative chemotherapeutics to treat the infections caused by MRSA after the confirmation of their cytotoxic and pharmacokinetic studies.
Evaluating the effectiveness of a novel somatostatin receptor 2 antagonist, ZT-01, for hypoglycemia prevention in a rodent model of type 2 diabetes
Authors: D’Souza Ninoschka C. , Aiken Julian A. , Hoffman Emily G. , Atherley Sara C. , Champsi Sabrina , Aleali Nadia , Shakeri Dorsa , El-Zahed Maya , Akbarian Nicky , Nejad-Mansouri Mehran , Bavani Parinaz Z. , Liggins Richard L. , Chan Owen , Riddell Michael C.
Published: February 2024

Background: Elevated levels of somatostatin blunt glucagon counterregulation during hypoglycemia in type 1 diabetes (T1D) and this can be improved using somatostatin receptor 2 (SSTR2) antagonists. Hypoglycemia also occurs in late-stage type 2 diabetes (T2D), particularly when insulin therapy is initiated, but the utility of SSTR2 antagonists in ameliorating hypoglycemia in this disease state is unknown. We examined the efficacy of a single-dose of SSTR2 antagonists in a rodent model of T2D.

Methods: High-fat fed (HFF), low dose streptozotocin (STZ, 35 mg/kg)-induced T2D and HFF only, nondiabetic (controls-no STZ) rats were treated with the SSTR2 antagonists ZT-01/PRL-2903 or vehicle (n = 9–11/group) 60 min before an insulin tolerance test (ITT; 2–12 U/kg insulin aspart) or an oral glucose tolerance test (OGTT; 2 g/kg glucose via oral gavage) on separate days.

Results: This rodent model of T2D is characterized by higher baseline glucose and HbA1c levels relative to HFF controls. T2D rats also had lower c-peptide levels at baseline and a blunted glucagon counterregulatory response to hypoglycemia when subjected to the ITT. SSTR2 antagonists increased the glucagon response and reduced incidence of hypoglycemia, which was more pronounced with ZT-01 than PRL-2903. ZT-01 treatment in the T2D rats increased glucagon levels above the control response within 60 min of dosing, and values remained elevated during the ITT (glucagon Cmax: 156 ± 50 vs. 77 ± 46 pg/mL, p < 0.01). Hypoglycemia incidence was attenuated with ZT-01 vs. controls (63% vs. 100%) and average time to hypoglycemia onset was also delayed (103.1 ± 24.6 vs. 66.1 ± 23.6 min, p < 0.05). ZT-01 administration at the OGTT onset increased the glucagon response without exacerbating hyperglycemia (2877 ± 806 vs. 2982 ± 781), potentially due to the corresponding increase in c-peptide levels (6251 ± 5463 vs. 14008 ± 5495, p = 0.013).

Conclusion: Treatment with SSTR2 antagonists increases glucagon responses in a rat model of T2D and results in less hypoglycemia exposure. Future studies are required to determine the best dosing periods for chronic SSTR2 antagonism treatment in T2D.
Superior haplotypes towards the development of blast and bacterial blight-resistant rice
Authors: Alam Shamshad , Sundaram Krishna Tesman , Singh Uma Maheshwar , Srinivas Prasad Madamshetty , Laha Gouri Sankar , Sinha Pallavi , Singh Vikas Kumar
Published: February 2024

Rice blast and bacterial leaf blight, are major disease, significantly threatens rice yield in all rice growing regions under favorable conditions and identification of resistance genes and their superior haplotypes is a potential strategy for effectively managing and controlling this devastating disease. In this study, we conducted a genome-wide association study (GWAS) using a diverse set of 147 rice accessions for blast and bacterial blight diseases in replications. Results revealed 23 (9 for blast and 14 for BLB) significant marker-trait associations (MTAs) that corresponded to 107 and 210 candidate genes for blast and BLB, respectively. The haplo-pheno analysis of the candidate genes led to the identification of eight superior haplotypes for blast, with an average SES score ranging from 0.00 to 1.33, and five superior haplotypes for BLB, with scores ranging from 1.52cm to 4.86cm superior haplotypes. Among these, superior haplotypes LOC_OS12G39700-H4 and LOC_Os06g30440-H33 were identified with the lowest average blast scores of 0.00-0.67, and superior haplotype LOC_Os02g12660-H39 exhibited the lowest average lesion length (1.88 - 2.06cm) for BLB. A total of ten accessions for blast and eight accessions for BLB were identified carrying superior haplotypes were identified. These haplotypes belong to aus and indx subpopulations of five countries (Bangladesh, Brazil, India, Myanmar, and Pakistan). For BLB resistance, eight accessions from six countries (Bangladesh, China, India, Myanmar, Pakistan, and Sri Lanka) and four subpopulations (aus, ind1A, ind2, and ind3) were identified carrying superior haplotypes. Interestingly, four candidate genes, LOC_Os06g21040, LOC_Os04g23960, LOC_Os12g39700, and LOC_Os01g24640 encoding transposon and retrotransposon proteins were among those with superior haplotypes known to play a crucial role in plant defense responses. These identified superior haplotypes have the potential to be combined into a single genetic background through haplotype-based breeding for a broader resistance spectrum against blast and bacterial blight diseases.
Regulation of tissue growth in plants – A mathematical modeling study on shade avoidance response in Arabidopsis hypocotyls
Authors: Favre Patrick , van Schaik Evert , Schorderet Martine , Yerly Florence , Reinhardt Didier
Published: February 2024

Introduction

Plant growth is a plastic phenomenon controlled both by endogenous genetic programs and by environmental cues. The embryonic stem, the hypocotyl, is an ideal model system for the quantitative study of growth due to its relatively simple geometry and cellular organization, and to its essentially unidirectional growth pattern. The hypocotyl of Arabidopsis thaliana has been studied particularly well at the molecular-genetic level and at the cellular level, and it is the model of choice for analysis of the shade avoidance syndrome (SAS), a growth reaction that allows plants to compete with neighboring plants for light. During SAS, hypocotyl growth is controlled primarily by the growth hormone auxin, which stimulates cell expansion without the involvement of cell division.

Methods

We assessed hypocotyl growth at cellular resolution in Arabidopsis mutants defective in auxin transport and biosynthesis and we designed a mathematical auxin transport model based on known polar and non-polar auxin transporters (ABCB1, ABCB19, and PINs) and on factors that control auxin homeostasis in the hypocotyl. In addition, we introduced into the model biophysical properties of the cell types based on precise cell wall measurements.

Results and Discussion

Our model can generate the observed cellular growth patterns based on auxin distribution along the hypocotyl resulting from production in the cotyledons, transport along the hypocotyl, and general turnover of auxin. These principles, which resemble the features of mathematical models of animal morphogen gradients, allow to generate robust shallow auxin gradients as they are expected to exist in tissues that exhibit quantitative auxin-driven tissue growth, as opposed to the sharp auxin maxima generated by patterning mechanisms in plant development.
Meta-analysis of exercise intervention on health behaviors in middle-aged and older adults
Authors: Liu Min , Mei Dong-hui , Zhang Ya-lu , Kang Ning , Wang Dong-min , Chen Gong
Published: February 2024

Objective

To systematically review and analyze the effects of exercise interventions on health behavior among middle-aged and older adults.

Methods

A Meta-analysis was performed using NoteExpress software to screen randomized controlled trials (RCTs) published between January 1, 2000, and March 31, 2023, which were identified through databases including CNKI, Web of Science, Pubmed, and 6 more sources, based on predefined literature inclusion criteria. Following the quality assessment, we conducted both the overall and subgroup Meta-analyses of the exercise intervention moderator variables using Review Manager 5.4.1 software, encompassing data among the 18 RCTs. The effect size was measured as the standardized mean difference within its corresponding 95% confidence interval. Heterogeneity was assessed using the I2 metrics.

Results

(1) The overall results indicate a significant impact of exercise intervention on health behaviors, characterized by a large effect size [SMD = 1.02 > 0.8, 95% CI (0.73, 1.32), P < 0.001]. (2) The highest degree of heterogeneity in the relationship between exercise interventions and health behaviors was associated with the duration of one exercise session (I2= 71.2%), which was the most influential moderator variable. (3) The aerobic and resistance intervention in group exercise lasting 30–60 min per time a day, 6–7 times per week over a period of 8–12 weeks demonstrated the most substantial effect size.

Conclusion

(1) The exercise intervention significantly promotes the health behavior in middle-aged and older adults, emphasizing the importance of carefully considering the duration of individual exercise sessions when designing and implementing exercise intervention. (2) Considering the accessibility for middle-aged and older adults, the optimal exercise intervention should include the means of group practice, the types of aerobic and resistance exercise, with a duration of 30–60 min per time a day, beginning with a lower weekly frequency and gradually increase to 6–7 times per week, and lasting for 8 to 12 weeks.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42024506750.
COVID-19 vaccine hesitancy and attitudes of subjects with disability and their carers in Saudi Arabia: a cross-sectional study
Authors: Sayed Anwar A.
Published: February 2024

The COVID-19 pandemic has caused significant disruption to countries worldwide, including Saudi Arabia. The fast preventative measures and the mass vaccine enrollment were vital to contain the pandemic in the country. However, vaccine hesitancy was a significant obstacle to taking the vaccine but was not previously explored. One hundred eighty-six subjects with disabilities were enrolled in this study in an attempt to explore their hesitancy and attitudes toward COVID-19 vaccines. Most participants were previously diagnosed with COVID-19 and had a close family who was also diagnosed with it. Most of them were willing to be vaccinated but had not received previous vaccinations. Official sources of information, e.g., TV/radio, were an essential factor driving their intention to get vaccinated. Beliefs that drove participants’ vaccine acceptance included vaccine safety, sufficient testing before its release, and its ability to protect from infection. The results of this seminal study provide insights to public health policymakers, which should be considered and taken together in light of other studies addressing the population’s vaccine hesitancy.
Sensory and nutritional evaluation of nine types of millet substituted for polished white rice in select Indian meal preparations
Authors: Anitha Seetha , Arjun Priya , Palli Nagarekha C. , Sreekanth N. , Miruthika Devi S. A. , Pandey Sangeeta , Krishnan Sridhar , Prasad Shyam , Sharma Shashi , Chidambara Murthy K. N. , Botha Rosemary , Upadhyay Shweta , Kane-Potaka Joanna
Published: February 2024

This study was conducted to test the suitability of using nine types of millets namely finger millet, pearl millet, white and yellow sorghum, little millet, barnyard millet, proso millet, kodo millet, and browntop millet in seven popular Indian meal preparations based on sensory characteristics and nutrient value. The popular Indian meal preparations tested were boiled grain, dosa, idli, bisi belle bath, pulao, puttu, and pongal. In total, 53 variations in meal preparations were developed using the millets and seven polished white rice-based meal preparations were developed as control. The main findings indicated that meal preparation crafted from various millets garnered overall sensory scores closely resembling to those derived from polished white rice. Notably, little millet exhibited high scores in pongal and dosa, and achieved elevated overall sensory scores compared to meal preparation from polished white rice. Bisi belle bath made of barnyard millet scored higher in overall sensory score than polished white rice. Moreover, there was significant association between some types of millets’ overall sensory characteristics (p < 0.005) with polished white rice-based meal preparations. In terms of nutrient value, all the millet-based meal preparations had significantly high nutritional value compared to those made with polished white rice (p < 0.05). Especially calcium content of the meal prepared with finger millet was significantly higher compared to polished white rice-based meals (p < 0.05). Puttu, idli, and dosa prepared with finger millet had calcium content of 59.4, 10.8, and 70.9 mg/100 g compared to those prepared with the polished white rice which had only 1.3, 6.3, and 9.2 mg/100 g. The magnesium content of all millet-based meal preparations was generally several-folds higher compared to the polished white rice-based meal preparations (p < 0.05). There is a significant difference in the fiber content of the meals prepared with millets compared to the meals prepared with polished white rice (p < 0.05). This study was conducted using millets that are locally available and does not represent all the millet varieties available globally, as each type of millet has a wide range of varieties. Therefore, it is important to understand and choose the type and variety of millet while enhancing the nutritional value of diets.