bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated Clarification and Purification of Monoclonal Antibodies by Membrane Based Separation of Aqueous Two-Phase Systems
Authors: Kruse Thomas , Schmidt Axel , Kampmann Markus , Strube Jochen
Published: July 2019

Therapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for the subsequent clarification and capture operations in the downstream process. As an alternative approach to the conventional downstream process, a selective mAb extraction via an aqueous two-phase system (ATPS) directly from the cultivation broth of a mAb producing industrial relevant chinese hamster ovary (CHO) cell line was investigated. An efficient purification of the mAb was accomplished by the ATPS composition. The phase separation was realized by a newly developed membrane based phase separator. Moreover, a complete cell removal was integrated into this process by the used membrane. A selectivity between both phases was achieved by membrane modification. Yields up to 93% in the light phase and removal of process related impurities were obtained after aqueous two-phase extraction (ATPE). Phase separation performance as well as contact angles on the membrane were characterized for different ATPS. ATPE directly from the cultivation broth in combination with the new membrane based phase separation led to a mAb yield of 78% with a simultaneous reduction of deoxyribonucleic acid (DNA) and host cell protein (HCP) load.
Optimization of Ultrasound-Assisted Extraction of Polyphenols from Myrtus communis L. Pericarp
Authors: Bouaoudia-Madi Nadia , Boulekbache-Makhlouf Lila , Madani Khodir , Silva Artur M.S. , Dairi Sofiane , Oukhmanou–Bensidhoum Sonia , Cardoso Susana M.
Published: July 2019

Response surface methodology (RSM) was used to optimize the extraction of phenolics from pericap of Myrtus communis using ultrasound-assisted extraction (UAE). The results were compared with those obtained by microwave-assisted extraction (MAE) and conventional solvent extraction (CSE) methods. The individual compounds of the optimized extract obtained by UAE were identified by ultra-high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (UHPLC-DAD-ESI-MSn). The yield of total phenolic compounds (TPC) was affected more significantly by ethanol concentration, irradiation time, liquid solvent-to-solid ratio (p < 0.0001) and amplitude (p = 0.0421) and optimal parameters conditions set by the RSM model were 70% (v/v), 7.5 min and 30%, respectively. The experimental yield of TPC (241.66 ± 12.77 mg gallic acid equivalent/g dry weight) confirmed the predicted value (235.52 ± 9.9 mg gallic acid equivalent/g dry weight), allowing also to confirm the model validity. Under optimized conditions, UAE was more efficient than MAE and CSE in extracting antioxidants, which comprised mostly myricetin glycosides. Globally, the present work demonstrated that, compared to MAE and CSE, UAE is an efficient method for phenolic extraction from M. communis pericarp, enabling to reduce the working time and the solvent consumption.
Devices and Protocols for Upper Limb Robot-Assisted Rehabilitation of Children with Neuromotor Disorders
Authors: Falzarano Valeria , Marini Francesca , Morasso Pietro , Zenzeri Jacopo
Published: July 2019

Neuromotor disorders negatively affect the sensorimotor system, limiting the ability to perform daily activities autonomously. Rehabilitation of upper limb impairments is therefore essential to improve independence and quality of life. In the last two decades, there has been a growing interest in robot-assisted rehabilitation as a beneficial way to promote children recovery process. However, a common understanding of the best drivers of an effective intervention has not been reached yet. With this aim, the current study reviewed the existing literature on robot-assisted rehabilitation protocols for upper extremities in children, with the goal of examining the effects of robotic therapy on their sensorimotor recovery process. A literature search was conducted in several electronic database to identify the studies related to the application of robotic therapy on upper limbs in the pediatric population. We analyzed three reviews and 35 studies that used 14 different robotic devices, and an overview of their characteristics, applications in the clinical setting and results is provided. Besides, the potential benefits of robot-assisted assessment and therapy are discussed to identify the key factors yielding positive outcomes in children. Finally, this review aim to lay the foundations for more effective neuroplasticity-enhancement protocols and elicit insights into robot-based approaches.
Modeling of Fatigue-Strength Development in Cold-Emulsion Asphalt Mixtures Using Maturity Method
Authors: Chelelgo Kiplagat , Gariy Zachary C. Abiero , Shitote Stanley Muse
Published: July 2019

Emulsion asphalts are cost-effective, environmentally friendly, and sustainable alternatives to hot-mix asphalts. Laboratory curing protocols currently used to simulate field curing of emulsion asphalts have been observed to offer conflicting predictions. This study employed the maturity method to account for the combined effects of temperature and time on fatigue-strength development in emulsion asphalts. An emulsion asphalt, composed of 55% reclaimed asphalt pavement, 45% virgin aggregates, 6.2% bitumen emulsion, and 4% pre-mix water was designed following the Asphalt Institute procedure. A total of 168 specimens from the mix were variously cured at 5 °C, 25 °C, 40 °C, and 50 °C for time intervals of 1, 3, 5, 7, 14, 21, and 28 days, before being tested for fatigue-strengths on the four-point bending test jig. It was observed that fatigue-strengths increased with an increase in cure temperature but decreased with an increase in cure duration. A parabolic hyperbolic fatigue-maturity model was developed from results of specimens cured at 5 °C, 25 °C, and 40 °C and validated with results from specimens cured at 50 °C. A strong correlation was observed between predicted fatigue-maturity and laboratory-determined fatigue-strengths at test strain levels between 125 µm/m and 200 µm/m. The study concluded that the laboratory characterization of emulsion asphalts should consider the curing history of the mix.
Measurement System of Metal Magnetic Memory Method Signals around Rectangular Defects of a Ferromagnetic Pipe
Authors: Villegas-Saucillo J. Jesús , Díaz-Carmona José Javier , Cerón-Álvarez Carlos A. , Juárez-Aguirre Raúl , Domínguez-Nicolás Saúl M. , López-Huerta Francisco , Herrera-May Agustín L.
Published: July 2019

Oil and gas pipeline networks require the periodic inspection of their infrastructure, which can cause gas and oil leakage with several damages to the environment and human health. For this, non-destructive testing (NDT) techniques of low-cost and easy implementation are required. An option is the metal magnetic memory (MMM) method, which could be used for real-time monitoring defects of ferromagnetic structures based on the analysis of self-magnetic leakage fields distribution around each defect. This method only requires magnetic sensors with high resolution and a data processing system. We present a measurement system of tangential and normal MMM signals of three rectangular defects of an ASTM A-36 steel pipe. This system is formed by a magnetoresistive sensor, an Arduino nano and a virtual instrumentation. The measured magnetic signals have non-uniform distributions around the rectangular defects, which have small differences with respect to the results obtained of a 2D magnetic dipole model. The size of each rectangular defect is related to the amplitude and shape of its tangential and normal MMM signals. The proposed system could be used for real-time monitoring of the size and location of rectangular defects of ferromagnetic pipes. This system does not require expensive equipment, operators with high skill level or a special treatment of the ferromagnetic samples.
Evaluation of Three Auditory-Sculptural Qualities Created by an Icosahedral Loudspeaker
Authors: Sharma Gerriet K. , Frank Matthias , Zotter Franz
Published: July 2019

The icosahedral loudspeaker (IKO) was previously established as an electroacoustic instrument enabling the musical creation and orchestration of sculptural sound phenomena in the room. This is technically achieved by manipulating the strengths of the available acoustic reflection paths by using the IKO’s acoustic beamforming capabilities. In its use, listeners perceive auditory sculptures whose characterization needs investigation. We present a proposed set of sculptural quality attributes directionality, contour, and plasticity and a series of listening experiments investigating them. The experiments employ documented beam layouts using a selected set of sounds as conditions, and they evaluate the recognizability, perceivable grading, and discernibility of the proposed sculptural qualities.
Antioxidant Properties of Thai Traditional Herbal Teas
Authors: Tipduangta Pratchaya , Julsrigival Jakaphun , Chaithatwatthana Kritsaya , Pongterdsak Nusrin , Tipduangta Pramote , Chansakaow Sunee
Published: July 2019

Recipes for Thai traditional herbal teas have been formulated based on Thai traditional medicine, which tries to achieve a balance of the body’s elements (fire, water, wind and earth) in each season. This study aims to assess the benefits of Thai traditional herbal teas through evaluating their antioxidant properties and measuring the satisfaction levels of healthy volunteers. Antioxidant activity tests performed on 11 tea recipes and on green tea (positive control) included 2,2-diphenyl-1-picrylhydrazyl (DPPH), a radical scavenging assay and a 2,2’-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) assay. In addition, total phenolic contents were determined using a Folin-Ciocalteu assay. Tests of satisfaction were performed on volunteer subjects aged between 18 and 30 years old, using the criteria of color, flavor, taste and overall satisfaction. Among the 11 tea recipes tested, the summer recipe 1 containing Aegle marmelos (L.) Correa, Stevia rebaudiana (Bert.) Bertoni, Pandanus amaryllifolius Roxb. and Morus alba L. demonstrated the most promising antioxidant properties (p > 0.05) and a phenolic content (p > 0.05), which was comparable to green tea. Furthermore, it got the highest satisfaction scores in every assessed category. Therefore, this study shows that Thai traditional herbal teas, developed based on the concepts of Thai traditional medicine, could be a good choice for people looking for health-promoting beverages.
Monitoring Radiotherapeutic Response in Prostate Cancer Patients Using High Throughput FTIR Spectroscopy of Liquid Biopsies
Authors: Medipally Dinesh K.R. , Nguyen Thi Nguyet Que , Bryant Jane , Untereiner Valérie , Sockalingum Ganesh D. , Cullen Daniel , Noone Emma , Bradshaw Shirley , Finn Marie , Dunne Mary , Shannon Aoife M. , Armstrong John , Lyng Fiona M. , Meade Aidan D.
Published: July 2019

Radiation therapy (RT) is used to treat approximately 50% of all cancer patients. However, RT causes a wide range of adverse late effects that can affect a patient’s quality of life. There are currently no predictive assays in clinical use to identify patients at risk of normal tissue radiation toxicity. This study aimed to investigate the potential of Fourier transform infrared (FTIR) spectroscopy for monitoring radiotherapeutic response. Blood plasma was acquired from 53 prostate cancer patients at five different time points: prior to treatment, after hormone treatment, at the end of radiotherapy, two months post radiotherapy and eight months post radiotherapy. FTIR spectra were recorded from plasma samples at all time points and the data was analysed using MATLAB software. Discrimination was observed between spectra recorded at baseline versus follow up time points, as well as between spectra from patients showing minimal and severe acute and late toxicity using principal component analysis. A partial least squares discriminant analysis model achieved sensitivity and specificity rates ranging from 80% to 99%. This technology may have potential to monitor radiotherapeutic response in prostate cancer patients using non-invasive blood plasma samples and could lead to individualised patient radiotherapy.
Wnt/β-Catenin Signaling in Liver Cancers
Authors: Wang Wenhui , Smits Ron , Hao Haiping , He Chaoyong
Published: July 2019

Liver cancer is among the leading global healthcare issues associated with high morbidity and mortality. Liver cancer consists of hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA), hepatoblastoma (HB), and several other rare tumors. Progression has been witnessed in understanding the interactions between etiological as well as environmental factors and the host in the development of liver cancers. However, the pathogenesis remains poorly understood, hampering the design of rational strategies aiding in preventing liver cancers. Accumulating evidence demonstrates that aberrant activation of the Wnt/β-catenin signaling pathway plays an important role in the initiation and progression of HCC, CCA, and HB. Targeting Wnt/β-catenin signaling potentiates a novel avenue for liver cancer treatment, which may benefit from the development of numerous small-molecule inhibitors and biologic agents in this field. In this review, we discuss the interaction between various etiological factors and components of Wnt/β-catenin signaling early in the precancerous lesion and the acquired mechanisms to further enhance Wnt/β-catenin signaling to promote robust cancer formation at later stages. Additionally, we shed light on current relevant inhibitors tested in liver cancers and provide future perspectives for preclinical and clinical liver cancer studies.
The Use of Optimal Treatment for DLBCL Is Improving in All Age Groups and Is a Key Factor in Overall Survival, but Non-Clinical Factors Influence Treatment
Authors: Wong Doo Nicole , White Victoria M. , Martin Kara , Bassett Julie K. , Prince H. Miles , Harrison Simon J. , Jefford Michael , Winship Ingrid , Millar Jeremy L. , Milne Roger L. , Seymour John F. , Giles Graham G.
Published: July 2019

Introduction: Diffuse large B cell lymphoma (DLBCL) is an aggressive form of non-Hodgkin lymphoma for which a cure is usually the therapeutic goal of optimal treatment. Using a large population-based cohort we sought to examine the factors associated with optimal DLBCL treatment and survival. Methods: DLBCL cases were identified through the population-based Victorian Cancer Registry, capturing new diagnoses for two time periods: 2008–2009 and 2012–2013. Treatment was pre-emptively classified as ‘optimal’ or ‘suboptimal’, according to compliance with current treatment guidelines. Univariable and multivariable logistic regression models were fitted to determine factors associated with treatment and survival. Results: Altogether, 1442 DLBCL cases were included. Based on multivariable analysis, delivery of optimal treatment was less likely for those aged ≥80 years (p < 0.001), women (p = 0.012), those with medical comorbidity (p < 0.001), those treated in a non-metropolitan hospital (p = 0.02) and those who were ex-smokers (p = 0.02). Delivery of optimal treatment increased between 2008–2009 and the 2012–2013 (from 60% to 79%, p < 0.001). Delivery of optimal treatment was independently associated with a lower risk of death (hazard ratio (HR) = 0.60 (95% confidence interval (CI) 0.45–0.81), p = 0.001). Conclusion: Delivery of optimal treatment for DLBCL is associated with hospital location and category, highlighting possible demographic variation in treatment patterns. Together with an increase in the proportion of patients receiving optimal treatment in the more recent time period, this suggests that treatment decisions in DLBCL may be subject to non-clinical influences, which may have implications when evaluating equity of treatment access. The positive association with survival emphasizes the importance of delivering optimal treatment in DLBCL.
STAT5a/b Deficiency Delays, but does not Prevent, Prolactin-Driven Prostate Tumorigenesis in Mice
Authors: Boutillon Florence , Pigat Natascha , Sackmann Sala Lucila , Reyes-Gomez Edouard , Moriggl Richard , Guidotti Jacques-Emmanuel , Goffin Vincent
Published: July 2019

The canonical prolactin (PRL) Signal Transducer and Activator of Transcription (STAT) 5 pathway has been suggested to contribute to human prostate tumorigenesis via an autocrine/paracrine mechanism. The probasin (Pb)-PRL transgenic mouse models this mechanism by overexpressing PRL specifically in the prostate epithelium leading to strong STAT5 activation in luminal cells. These mice exhibit hypertrophic prostates harboring various pre-neoplastic lesions that aggravate with age and accumulation of castration-resistant stem/progenitor cells. As STAT5 signaling is largely predominant over other classical PRL-triggered pathways in Pb-PRL prostates, we reasoned that Pb-Cre recombinase-driven genetic deletion of a floxed Stat5a/b locus should prevent prostate tumorigenesis in so-called Pb-PRLΔSTAT5 mice. Anterior and dorsal prostate lobes displayed the highest Stat5a/b deletion efficiency with no overt compensatory activation of other PRLR signaling cascade at 6 months of age; hence the development of tumor hallmarks was markedly reduced. Stat5a/b deletion also reversed the accumulation of stem/progenitor cells, indicating that STAT5 signaling regulates prostate epithelial cell hierarchy. Interestingly, ERK1/2 and AKT, but not STAT3 and androgen signaling, emerged as escape mechanisms leading to delayed tumor development in aged Pb-PRLΔSTAT5 mice. Unexpectedly, we found that Pb-PRL prostates spontaneously exhibited age-dependent decline of STAT5 signaling, also to the benefit of AKT and ERK1/2 signaling. As a consequence, both Pb-PRL and Pb-PRLΔSTAT5 mice ultimately displayed similar pathological prostate phenotypes at 18 months of age. This preclinical study provides insight on STAT5-dependent mechanisms of PRL-induced prostate tumorigenesis and alternative pathways bypassing STAT5 signaling down-regulation upon prostate neoplasia progression.
Myasthenia Gravis: Pathogenic Effects of Autoantibodies on Neuromuscular Architecture
Authors: Koneczny Inga , Herbst Ruth
Published: July 2019

Myasthenia gravis (MG) is an autoimmune disease of the neuromuscular junction (NMJ). Autoantibodies target key molecules at the NMJ, such as the nicotinic acetylcholine receptor (AChR), muscle-specific kinase (MuSK), and low-density lipoprotein receptor-related protein 4 (Lrp4), that lead by a range of different pathogenic mechanisms to altered tissue architecture and reduced densities or functionality of AChRs, reduced neuromuscular transmission, and therefore a severe fatigable skeletal muscle weakness. In this review, we give an overview of the history and clinical aspects of MG, with a focus on the structure and function of myasthenic autoantigens at the NMJ and how they are affected by the autoantibodies’ pathogenic mechanisms. Furthermore, we give a short overview of the cells that are implicated in the production of the autoantibodies and briefly discuss diagnostic challenges and treatment strategies.
Effect of Additives Ag and Rare‐Earth Elements Y and Sc on the Properties of Hydrogen Sensors Based on Thin SnO2 Films during Long‐Term Testing
Authors: Maksimova Nadezhda K. , Almaev Aleksei V. , Sevastyanov Evgeniy Yu. , Potekaev Aleksandr I. , Chernikov Evgeniy V. , Sergeychenko Nadezhda V. , Korusenko Petr M. , Nesov Sergey N.
Published: July 2019

The paper presents the results of an investigation of the nanostructure, elements, and phase composition of thin (100–140 nm) tin dioxide films obtained via magnetron sputtering and containing Ag, Y, Sc, Ag + Y, and Ag + Sc additives in the volume. Electrical and gas‐sensitive characteristics of hydrogen sensors based on these films with dispersed Pt/Pd layers deposited on the surface were studied. The additives had a significant effect on the nanostructure of the films, the density of oxygen adsorption sites on the surface of tin dioxide, the band bending at the grain boundaries of tin dioxide, the resistance values in pure air, and the responses to hydrogen in the concentration range of 50–2000 ppm. During the long‐term tests of most of the samples studied, there was an increase in the resistance of the sensors in clean air and in the response to hydrogen. It has been established that the joint introduction of Ag + Y into the volume of films prevents the increase in the resistance and response. For these sensors based on thin films of Pt/Pd/SnO2:Sb, Ag, Y the responses to 100 and 1000 ppm of H2 are 25 and 575, correspondingly, the response time at exposure to 100 and 1000 ppm of H2 are 10 and 90 s, the recovery time at exposure to 100 and 1000 ppm of H2 17 and 125 s. Possible mechanisms of the effect of additives on the properties of sensors and the stability of their parameters during long‐term operation were considered.
Lipid-Rich Necrotic Core of Basilar Artery Atherosclerotic Plaque: Contrast-Enhanced Black Blood Imaging on Vessel Wall Imaging
Authors: Lee Young Kwang , Kwak Hyo Sung , Chung Gyung Ho , Hwang Seung Bae
Published: July 2019

Purpose: We wished to evaluate the lipid-rich necrotic core (LRNC) using contrast-enhanced T1-weighted (CE-T1W) black-blood (BB) imaging for vessel walls. Methods: Ninety-five patients with basilar artery (BA) stenosis who underwent magnetic resonance angiography between January 2016 and August 2018 were enrolled into this present study. CE-T1W BB imaging was considered as a reference method for identifying an LRNC. Results: Ten (10.5%) patients were identified as having an LRNC on CE-T1W BB imaging. Of these patients, 9 had acute symptoms. The extent of stenosis in patients with an LRNC on CE-T1W BB imaging was significantly greater than that of patients without an LRNC (p < 0.001). The maximum wall thickness in patients with an LRNC on CE-T1W imaging was significantly thicker than that of patients without an LRNC (p = 0.008). Conclusions: Identification of an LRNC on CE-T1W BB imaging was associated with high-grade stenosis and massive plaque burden from BA atherosclerosis.
Improving Parameter Estimation of Entropic Uncertainty Relation in Continuous-Variable Quantum Key Distribution
Authors: Chen Ziyang , Zhang Yichen , Wang Xiangyu , Yu Song , Guo Hong
Published: July 2019

The entropic uncertainty relation (EUR) is of significant importance in the security proof of continuous-variable quantum key distribution under coherent attacks. The parameter estimation in the EUR method contains the estimation of the covariance matrix (CM), as well as the max-entropy. The discussions in previous works have not involved the effect of finite-size on estimating the CM, which will further affect the estimation of leakage information. In this work, we address this issue by adapting the parameter estimation technique to the EUR analysis method under composable security frameworks. We also use the double-data modulation method to improve the parameter estimation step, where all the states can be exploited for both parameter estimation and key generation; thus, the statistical fluctuation of estimating the max-entropy disappears. The result shows that the adapted method can effectively estimate parameters in EUR analysis. Moreover, the double-data modulation method can, to a large extent, save the key consumption, which further improves the performance in practical implementations of the EUR.
Results Analysis of Russian Students’ Participation in the Online International Educational Project X-Culture
Authors: Zakharova Irina , Kobicheva Aleksandra , Rozova Natalia
Published: July 2019

The purpose of the paper is to assess the experience of Russian students in the online project class. The authors studied the impact of the international project “X-Culture” on the level of development of socio-cultural competencies of students and their knowledge of the English language. An additional aspect of the analysis was the question of the connection of students’ perception of the goal of participation in the international project “X-Culture” with their self-motivation and self-assessment of the success of the results achieved. The study used both quantitative and qualitative methods—student testing, database analysis provided by the project “X-culture”, and focus group records. The result of the participation has been inconsistent. As expected, such sociocultural competence as the level of proficiency in English increased among the majority of students, as confirmed by the values of the student’s criterion for the results of language testing, conducted before and after the project. At the same time, average indicators of sociocultural competences such as “interpersonal skills”, “creativity”, “leadership”, and “friendliness” have deteriorated during the project that is confirmed by the trends lines of time series. The focus group revealed differences in terms of participation in the X-Culture project, their connection with self-motivation, and student satisfaction with the results achieved. Students who have achieved high levels of sociocultural competences have set themselves the goals of participation in the project related to the improvement of professional competencies and intercultural communications. At the same time, students were most satisfied with their activities in the project and the results achieved, aimed at obtaining new professional knowledge and skills, and students whose goal was simply to obtain an international certificate of the project participant.
Machine Learning and Embedded Computing in Advanced Driver Assistance Systems (ADAS)
Authors: Ball John E. , Tang Bo
Published: July 2019

Advanced driver assistance systems (ADAS) are rapidly being developed for autonomous vehicles [...]
Soft-Error Vulnerability Estimation Approach Based on the SET Susceptibility of Each Gate
Authors: Batagin Armelin Fábio , Alves de Barros Naviner Lírida , d’Amore Roberto
Published: July 2019

Soft-Error Vulnerability (SEV) is a parameter used to evaluate the robustness of a circuit to the induced Soft Errors (SEs). There are many techniques for SEV estimation, including analytical, electrical and logic simulations, and emulation-based approaches. Each of them has advantages and disadvantages regarding estimation time, resources consumption, accuracy, and restrictions over the analysed circuit. Concerning the ionising radiation effects, some analytical and electrical simulation approaches take into account how the circuit topology and the applied input patterns affect their susceptibilities to Single Event Transient (SET) at the gate level. On the other hand, logic simulation and emulation techniques usually ignore these SET susceptibilities. In this context, we propose a logic simulation-based probability-aware approach for SEV estimation that takes into account the specific SET susceptibility of each circuit gate. For a given operational scenario, we extract the input patterns applied to each gate and calculate its specific SET susceptibility. For the 38 analysed benchmark circuits, we obtained a reduction from 15.27% to 0.68% in the average SEV estimation error, when comparing the estimated value to a reference obtained at the transistor level. The results point out an improvement of the SEV estimation process by considering the specific SET susceptibilities.
Numerical Simulation of Crystalline Silicon Heterojunction Solar Cells with Different p-Type a-SiOx Window Layer
Authors: Hsu Chia-Hsun , Zhang Xiao-Ying , Lin Hai-Jun , Lien Shui-Yang , Cho Yun-Shao , Ye Chang-Sin
Published: July 2019

In this study, p-type amorphous silicon oxide (a-SiOx) films are deposited using a radio-frequency inductively-coupled plasma chemical vapor deposition system. Effects of the CO2 gas flow rate on film properties and crystalline silicon heterojunction (HJ) solar cell performance are investigated. The experimental results show that the band gap of the a-SiOx film can reach 2.1 eV at CO2 flow rate of 10 standard cubic centimeters per minute (sccm), but the conductivity of the film deteriorates. In the device simulation, the transparent conducting oxide and contact resistance are not taken into account. The electrodes are assumed to be perfectly conductive and transparent. The simulation result shows that there is a tradeoff between the increase in the band gap and the reduction in conductivity at increasing CO2 flow rate, and the balance occurs at the flow rate of six sccm, corresponding to a band gap of 1.95 eV, an oxygen content of 34%, and a conductivity of 3.3 S/cm. The best simulated conversion efficiency is 25.58%, with an open-circuit voltage of 741 mV, a short-circuit current density of 42.3 mA/cm2, and a fill factor of 0.816%.
A Letter from the Editor-in-Chief to Our Readers
Authors: Sciubba Enrico
Published: July 2019

I would like to take the occasion of writing this newsletter to draw a sort of summary of our publishing activities at Energies and of the results and perspectives [...]
A Comparative Study of the Oil-Free Centrifugal Water Chillers with the Flooded or Falling Film Evaporator—A Case Study
Authors: Hung Kuo-Shu , Chung Jenn-Chyi , Liu Chung-Che , Lin Jun-Jie , Wang Chi-Chuan
Published: July 2019

A comparative study regarding the performance of real-scale oil-free centrifugal chillers having the flooded evaporator or falling film evaporator was conducted in this study. The nominal capacity for the test chillers was around 200~230 USRT (US refrigeration ton) (703~809 kW). The compressors of the two chillers were identical and R-134a was used as the working fluid. Both evaporators employed the same enhanced tubes (GEWA-B) to fulfill phase change. Tests were conducted in full, 75%, 50%, and 25% loading. Test results indicate that both chillers contained a comparable system performance with an integrated part-load value of around 8.62~8.63. The overall heat transfer coefficient for the flooded evaporator was appreciably higher (20~40%) than the falling film evaporator. This is because the falling film flowrate was below the threshold value and the heat transfer was dominated by evaporation mode. Yet, the heat transfer performance for the falling film evaporator was further jeopardized due to starvation of the film flowrate (partial dry-out), especially in the middle or bottom of the tube bundle. This phenomenon became even more pronounced at partial loading (25%), whereas the flooded evaporator did not reveal such a performance dip at partial loading.
Preliminary Design of Multistage Radial Turbines Based on Rotor Loss Characteristics under Variable Operating Conditions
Authors: Tong Zheming , Cheng Zhewu , Tong Shuiguang
Published: July 2019

The loading-to-flow diagram is a widely used classical method for the preliminary design of radial turbines. This study improves this method to optimize the design of radial turbines in the early design phase under variable operating conditions. The guide vane outlet flow angle is a key factor affecting the off-design performance of the radial turbine. To optimize the off-design performance of radial turbines in the early design phase, we propose a hypothesis that uses the ratio of the mean velocity of the fluid relative to the rotor passage with respect to the circumferential velocity of the rotor as an indicator to indirectly and qualitatively estimate the rotor loss, as it plays a key role in the off-design efficiency. Theoretical analysis of rotor loss characteristics under different types of variable operating conditions shows that a smaller design value of guide vane outlet flow angle results in a better off-design performance in the case of a reduced mass flow. In contrast, radial turbines with a larger design value of guide vane outlet flow angle can obtain a better off-design performance with increased mass flow. The above findings were validated with a mean-line model method. Furthermore, this study discusses the optimization of the design value of guide vane outlet flow angle based on the matching of rotor loss characteristics with specified variable operating conditions. It provides important guidance for the design optimization of multistage radial turbines with variable operating conditions in compressed air energy storage (CAES) systems.
Colonization of Pinus radiata D. Don Seedling Roots by Biocontrol Bacteria Erwinia billingiae and Bacillus simplex
Authors: Mesanza Nebai , Crawford Bryan D. , Coulson Thomas J.D. , Iturritxa Eugenia , Patten Cheryl L.
Published: July 2019

Erwinia billingiae S31R1 and Bacillus simplex S11R41, isolated from the rhizosphere of a healthy tree located in a Pinus radiata D. Don plantation with high presence of fungal pathogens, are antagonists of pine root rot fungi Heterobasidion annosum and Armillaria mellea in vitro and in young trees. For effective biocontrol of these pathogens, the bacteria must stably colonize P. radiata roots following their application. To determine root colonization patterns, the bacteria were transformed with stable plasmids encoding green fluorescent protein (GFP). Transformed E. billingiae was visualized on roots 24 days after soil inoculation by confocal and epifluorescence microscopy, and GFP was detected by ELISA 31 days after inoculation. The presence of E. billingiae microcolonies, in some cases in root intercellular spaces, suggests that bacterial growth was active and localized. Fluorescence of B. simplex S11R41 was visualized on P. radiata roots 31 days after inoculation and its colonization pattern changed from scattered cells to localized microcolonies. Although the populations decreased over time, microcolony formation and localization in specific regions of roots indicated that E. billingiae, normally considered to be an epiphyte, and B. simplex can stably colonize roots of P. radiata.
Warming Alters Plant Chemical and Nutrient Compositions by Affecting Metabolites in Cunninghamia lanceolata (Lamb.) Hook
Authors: Zhang Qiufang , Yang Zhijie , Chen Tingting , Gong Xiaoying , Xiong Decheng , Ye Wangmin , Chen Yuehmin , Yang Yusheng
Published: July 2019

Research Highlights: Warming alters the chemical composition of Cunninghamia lanceolata (Lamb.) Hook, resulting in increased production of macromolecular compounds that protect against heat stress. Background and Objectives: Low latitude forests are experiencing obvious climatic warming; however, the plant physiological responses to warming are not well understood. As warming induces moisture stress, we hypothesized that warming activates metabolites (i.e., lipids, phenolic compounds, amino acids) and causes damage to the leaves, exemplified by the increased concentrations of reactive oxygen species. Materials and Methods: We conducted a warming experiment in a C. lanceolata plantation. Plant physiological traits associated with nutrient status, reactive oxygen species, antioxidant enzymes species, and metabolites were measured. Results: Warming altered the chemical composition of C. lanceolata as it increased C:N ratios of leaves and roots. In particular, the concentrations of N and P in leaves and roots were significantly decreased under the warming condition, which might be related to the biomass production, namely, a dilution effect. Under the warming condition, most of the phospholipid compounds and proteins significantly increased. Leaf C, carbohydrates, amino acids, organic acids, flavonoids, and phenolic compounds were identified to have significantly lower concentrations under the warming treatment than those under the control treatment. These results suggested that moisture stress under the warming treatment may drive C deficiency and metabolic restriction in plants. Conclusions: Under the warming condition, C. lanceolata changed its energy utilization strategy and invested more resources to produce macromolecular compounds for protecting against heat stress. Warming in sub-tropical forests alters plant chemical properties, and thus may have an important consequence for nutrient cycling and soil C sequestration.
Most Southern Scots Pine Populations Are Locally Adapted to Drought for Tree Height Growth
Authors: Vizcaíno-Palomar Natalia , González-Muñoz Noelia , González-Martínez Santiago , Alía Ricardo , Benito Garzón Marta
Published: July 2019

Most populations of Scots pine in Spain are locally adapted to drought, with only a few populations at the southernmost part of the distribution range showing maladaptations to the current climate. Increasing tree heights are predicted for most of the studied populations by the year 2070, under the RCP 8.5 scenario. These results are probably linked to the capacity of this species to acclimatize to new climates. The impact of climate change on tree growth depends on many processes, including the capacity of individuals to respond to changes in the environment. Pines are often locally adapted to their environments, leading to differences among populations. Generally, populations at the margins of the species’ ranges show lower performances in fitness-related traits than core populations. Therefore, under expected changes in climate, populations at the southern part of the species’ ranges could be at a higher risk of maladaptation. Here, we hypothesize that southern Scots pine populations are locally adapted to current climate, and that expected changes in climate may lead to a decrease in tree performance. We used Scots pine tree height growth data from 15-year-old individuals, measured in six common gardens in Spain, where plants from 16 Spanish provenances had been planted. We analyzed tree height growth, accounting for the climate of the planting sites, and the climate of the original population to assess local adaptation, using linear mixed-effect models. We found that: (1) drought drove differences among populations in tree height growth; (2) most populations were locally adapted to drought; (3) tree height was predicted to increase for most of the studied populations by the year 2070 (a concentration of RCP 8.5). Most populations of Scots pine in Spain were locally adapted to drought. This result suggests that marginal populations, despite inhabiting limiting environments, can be adapted to the local current conditions. In addition, the local adaptation and acclimation capacity of populations can help margin populations to keep pace with climate change. Our results highlight the importance of analyzing, case-by-case, populations’ capacities to cope with climate change.
Evaluation of O3 Effects on Cumulative Photosynthetic CO2 Uptake in Seedlings of Four Japanese Deciduous Broad-Leaved Forest Tree Species Based on Stomatal O3 Uptake
Authors: Yamaguchi Masahiro , Kinose Yoshiyuki , Matsumura Hideyuki , Izuta Takeshi
Published: July 2019

The current level of tropospheric ozone (O3) is expected to reduce the net primary production of forest trees. Here, we evaluated the negative effects of O3 on the photosynthetic CO2 uptake of Japanese forest trees species based on their cumulative stomatal O3 uptake, defined as the phytotoxic O3 dose (POD). Seedlings of four representative Japanese deciduous broad-leaved forest tree species (Fagus crenata, Quercus serrata, Quercus mongolica var. crispula and Betula platyphylla var. japonica) were exposed to different O3 concentrations in open-top chambers for two growing seasons. The photosynthesis–light response curves (A-light curves) and stomatal conductance were measured to estimate the leaf-level cumulative photosynthetic CO2 uptake (ΣPn_est) and POD, respectively. The whole-plant-level ΣPn_est were highly correlated with the whole-plant dry mass increments over the two growing seasons. Because whole-plant growth is largely determined by the amount of leaf area per plant and net photosynthetic rate per leaf area, this result suggests that leaf-level ΣPn_est, which was estimated from the monthly A-light curves and hourly PPFD, could reflect the cumulative photosynthetic CO2 uptake of the seedlings per unit leaf area. Although the O3-induced reductions in the leaf-level ΣPn_est were well explained by POD in all four tree species, species-specific responses of leaf-level ΣPn_est to POD were observed. In addition, the flux threshold appropriate for the linear regression of the responses of relative leaf-level ΣPn_est to POD was also species-specific. Therefore, species-specific responses of cumulative photosynthetic CO2 uptake to POD could be used to accurately evaluate O3 impact on the net primary production of deciduous broad-leaved trees.
Experimental Investigation of the Behavior of Self-Form Segmental Concrete Masonry Arches
Authors: Abdulhameed Ali A. , Said AbdulMuttalib I.
Published: July 2019

This research aims to introduce a new technique—off-site and self-form segmental concrete masonry arches fabrication, without the need of construction formwork or centering. The innovative construction method in the current study encompasses two construction materials forms the self-form masonry arches, wedge-shape plain concrete voussoirs, and carbon fiber-reinforced polymer (CFRP) composites. The employment of CFRP fabrics was for two main reasons: bonding the voussoirs and forming the masonry arches. In addition, CFRP proved to be efficient for strengthening the extrados of the arch rings under service loadings. An experimental test was conducted on four sophisticated masonry arch specimens. The research parameters were the Keystone thickness and the strengthening of the self-form arch ring at the intrados. The major test finding was that the use of thicker Keystone alters the behavior of the self-form arch and considerably increases the load carrying capacity by 79%. Partial strengthening of the intrados with CFRP fabrics of typical arch ring Keystone resulted considerable increase in the debonding load of fabrication CFRP sheets by 81%, increase in the localized crushing load by 13% and considerably increase voussoir sliding load by 107%.
Landscape of Overlapping Gene Expression in The Equine Placenta
Authors: Dini Pouya , Norris Jamie , El-Sheikh Ali Hossam , Loux Shavahn C. , Carossino Mariano , Esteller-Vico Alejandro , Bailey Ernest , Kalbfleisch Theodore , Daels Peter , Ball Barry A.
Published: July 2019

Increasing evidence suggests that overlapping genes are much more common in eukaryotic genomes than previously thought. These different-strand overlapping genes are potential sense–antisense (SAS) pairs, which might have regulatory effects on each other. In the present study, we identified the SAS loci in the equine genome using previously generated stranded, paired-end RNA sequencing data from the equine chorioallantois. We identified a total of 1261 overlapping loci. The ratio of the number of overlapping regions to chromosomal length was numerically higher on chromosome 11 followed by chromosomes 13 and 12. These results show that overlapping transcription is distributed throughout the equine genome, but that distributions differ for each chromosome. Next, we evaluated the expression patterns of SAS pairs during the course of gestation. The sense and antisense genes showed an overall positive correlation between the sense and antisense pairs. We further provide a list of SAS pairs with both positive and negative correlation in their expression patterns throughout gestation. This study characterizes the landscape of sense and antisense gene expression in the placenta for the first time and provides a resource that will enable researchers to elucidate the mechanisms of sense/antisense regulation during pregnancy.
Power and Design Issues in Crossover-Based N-Of-1 Clinical Trials with Fixed Data Collection Periods
Authors: Wang Yanpin , Schork Nicholas J.
Published: July 2019

“N-of-1,” or single subject, clinical trials seek to determine if an intervention strategy is more efficacious for an individual than an alternative based on an objective, empirical, and controlled study. The design of such trials is typically rooted in a simple crossover strategy with multiple intervention response evaluation periods. The effect of serial correlation between measurements, the number of evaluation periods, the use of washout periods, heteroscedasticity (i.e., unequal variances among responses to the interventions) and intervention-associated carry-over phenomena on the power of such studies is crucially important for putting the yield and feasibility of N-of-1 trial designs into context. We evaluated the effect of these phenomena on the power of different designs for N-of-1 trials using analytical theory based on standard likelihood principles assuming an autoregressive lag 1, i.e., AR(1), serial correlation structure among the measurements as well as simulation studies. By evaluating the power to detect effects in many different settings, we show that the influence of serial correlation and heteroscedasticity on power can be substantial, but can also be mitigated to some degree through the use of appropriate multiple evaluation periods. We also show that the detection of certain types of carry-over effects can be heavily influenced by design considerations as well.
An Examination of Device Types and Features Used by Adult Electronic Nicotine Delivery System (ENDS) Users in the PATH Study, 2015–2016
Authors: Coleman Blair , Chang Joanne T. , Rostron Brian L. , Johnson Sarah E. , Das Babita , Del Valle-Pinero Arseima Y.
Published: July 2019

To date no study has reported U.S. nationally representative estimates of current ENDS users by device category (“open” vs. “closed” systems) nor their detailed use behaviors. We examined the proportion of current adult ENDS users (unweighted n = 2671) using either “closed” or “open” systems during Wave 3 (2015–2016) of the Population Assessment of Tobacco and Health (PATH) Study. Demographic characteristics, use patterns, and device characteristics were examined overall and by device type. Among the 5.0% of current users at Wave 3, 43.9% used closed systems and 53.7% used open systems. Compared to closed system users, open system users were more likely to be male (60.7% vs. 48.4%), aged 18–24 (30.4% vs. 21.4%), and non-Hispanic White (76.2% vs. 65.4%), recent former (9.9% vs. 5.6%) or long-term former (20.2% vs. 10.9%) smokers, and use ENDS daily (44.1% vs. 22.5%); they were less likely to be to be current daily smokers (31.7% vs. 48.0%) or never smokers (15.2% vs. 19.5%). Adult ENDS users were nearly evenly split on their use of closed versus open systems; however, several group differences were observed. Disentangling the relationship between device selection and subsequent use patterns remains a public health priority.
Validation of a Short-Form Version of the Danish Need for Recovery Scale against the Full Scale
Authors: Stevens Matthew L. , Crowley Patrick , Garde Anne H. , Mortensen Ole S. , Nygård Clas-Håkan , Holtermann Andreas
Published: July 2019

Introduction: The Need for Recovery (NFR) Scale facilitates the understanding of the factors that can lead to sustainable working and employability. Short-form scales can reduce the burden on researchers and respondents. Our aim was to create and validate a short-form Danish version of the NFR Scale. Methods: Two datasets were used to conduct the exploratory and confirmatory analyses. This was done using qualitative and quantitative methods. The exploratory phase identified several short-form versions of the Danish NFR Scale and evaluated the quality of each through the assessment of content, construct and criterion validity, and responsiveness. These evaluations were then verified through the confirmatory analysis, using the second dataset. Results: A short-form NFR scale consisting of three items (exhausted at the end of a work day, hard to find interest in other people after a work day, it takes over an hour to fully recover from a work day) showed excellent validity and responsiveness compared to the nine-item scale. Furthermore, a short-form consisting of just two items also showed excellent validity and good responsiveness. Conclusion: A short-form NFR scale, consisting of three items from the Danish NFR Scale, seems to be an appropriate substitute for the full nine-item scale.