bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of 5-hydroxytryptamine on the short-circuit current across the small intestine of the gerbil (Gerbillus cheesmani) in different dietary states
Authors: Al-Balool FY
Published: October 2021

The effects of serosally added 5-hydroxytryptamine (5-HT, 100 microM) on the short circuit-current (Isc) across jejunum and ileum taken from fed, starved and undernourished (Gerbillus cheesmani) were investigated. The effects of the neurotoxin, tetrodotoxin (TTX, 10 microM) on the basal Isc as well as on the maximum increase in Isc induced by 5-HT were also studied. There were regional variations in the basal Isc as well as in the way by which the small intestine responds to 5-HT. The basal Isc was greater in jejunum than in ileum and such differences were TTX-sensitive. The maximum increase in Isc, which results from addition of 5-HT, was higher in jejunum than in ileum under all three feeding conditions. TTX reduced the maximum increase in Isc induced by 5-HT across stripped and intact intestine of the two regions in the three nutritional states. The 5-HT-induced Isc in the jejunum of both starved and undernourished gerbils and in the ileum of starved animals was the function of both submucosal and myenteric plexus. In jejunum and ileum taken from starved and undernourished gerbils the 5-HT-induced Isc was both chloride- and bicarbonate-dependent. Thus the results indicated that both starvation and undernourishment increase that response and such increases were TTX-sensitive and both chloride- and bicarbonate-dependent.
Responsiveness and habituation of soluble ICAM-1 to acute psychosocial stress in men:
determinants and effect of stress-hemoconcentration
Authors: von Känel R , Preckel D , Kudielka BM , Fischer JE
Published: October 2021

We studied the psychophysiology of soluble intercellular adhesion molecule-1 (sICAM-1) in 25 apparently healthy middle-aged men who underwent an acute psychosocial stressor three times with one week apart. Measures of the biological stress response were obtained at week one and three. The magnitude of the sICAM-1 stress response showed no habituation between individual visits. At week one, cognitive stress appraisal independently predicted integrated sICAM-1 area under the curve (AUC) between rest, immediately post-stress, and 45 min and 105 min post-stress (beta=0.67, p=0.012, deltaR(2)=0.41). Diastolic blood pressure AUC (beta=-0.45, p=0.048, deltaR(2)=0.21) and heart rate AUC (beta=0.44, p=0.055, deltaR(2)=0.21) were independent predictors of sICAM-1 AUC at week three. Adjustment for hemoconcentration yielded a decrease in sICAM-1 levels from rest to post-stress (p<0.001). Stress responsiveness of plasma sICAM-1 was predicted by stress perception and hemodynamic reactivity and affected by stress-hemoconcentration but unrelated to cortisol reactivity and not readily adapting to repeated stress.
Blood pressure and heart rate variability response to noninvasive ventilation in patients with exacerbations of chronic obstructive pulmonary disease
Authors: Skyba P , Joppa P , Orolín M , Tkáčová R
Published: October 2021

Sympathetic activation and parasympathetic withdrawal are commonly observed during acute exacerbations of chronic obstructive pulmonary disease (COPD). We have demonstrated previously that noninvasive positive-pressure ventilation (NPPV) improves parasympathetic neural control of heart rate in patients with obstructive sleep apnea. We hypothesized that NPPV may exert such beneficial effects in COPD as well. Therefore, we assessed the acute effects of NPPV on systemic blood pressure and indexes of heart rate variability (HRV) in 23 patients with acute exacerbations of COPD. The measurements of HRV in the frequency domain were computed by an autoregressive spectral technique. The use of NPPV resulted in significant increases of oxygen saturation (from 89.2+/-1.0 to 92.4+/-0.9 %, p<0.001) in association with reductions in systolic and diastolic blood pressures and heart rate (from 147+/-3 to 138+/-3 mm Hg, from 86+/-2 to 81+/-2 mm Hg, from 85+/-3 to 75+/-2 bpm, p<0.001 for all variables), and increases in ln-transformed high frequency band of HRV (from 6.4+/-0.5 to 7.4+/-0.6 ms(2)/Hz, p<0.01). Reductions in heart rate and increases in ln-transformed HF band persisted after NPPV withdrawal. In conclusion, these findings suggest that NPPV may cause improvements in the neural control of heart rate in patients with acute exacerbations of COPD.
Morphological changes in the hippocampus following nicotine and kainic acid administration
Authors: Riljak V , Milotová M , Jandová K , Pokorný J , Langmeier M
Published: October 2021

Using histochemical analysis (NADPH-diaphorase, Fluoro-Jade B dye and bis-benzimide 33,342 Hoechst) we studied the influence of intraperitoneal administration of nicotine (NIC), kainic acid (KA) and combination of both these substances on hippocampal neurons and their changes. In experiments, 35-day-old male rats of the Wistar strain were used. Animals were pretreated with 1 mg/kg of nicotine 30 min prior to the kainic acid application (10 mg/kg). After two days, the animals were transcardially perfused with 4 % paraformaldehyde under deep thiopental anesthesia. Cryostat sections were stained to identify NADPH-diaphorase positive neurons that were then quantified in the CA1 and CA3 areas of the hippocampus, in the dorsal and ventral blades of the dentate gyrus and in the hilus of the dentate gyrus. Fluoro-Jade B positive cells were examined in the same areas in order to elucidate a possible neurodegeneration. In animals exposed only to nicotine the number of NADPH-diaphorase positive neurons in the CA3 area of the hippocampus and in the hilus of the dentate gyrus was higher than in controls. In contrast, KA administration lowered the number of NADPH-diaphorase positive cells in all studied hippocampal areas and in both blades of the dentate gyrus. Massive cell degeneration was observed in CA1 and CA3 areas of the hippocampus and in the hilus of the dentate gyrus after kainic acid administration. Animals exposed to kainic acid and pretreated with nicotine exhibited degeneration to a lesser extent and the number of NADPH-diaphorase positive cells was higher compared to rats, which were exposed to kainic acid only.
Integrative hypothesis for Huntington’s disease: a brief review on experimental evidence.
Authors: Pérez-De La Cruz V , Santamaría A
Published: October 2021

Huntington's disease (HD) is a demential, neurodegenerative inheritable disease affecting middle-aged patients. HD is characterized by uncontrolled choreiform movements, psychiatric symptoms and cognitive decline. Histopathological changes in HD brains reveal a considerable damage to basal ganglia, particularly affecting middle-sized spiny neurons from the caudate-putamen region. Neurochemical changes are specifically oriented to deplete GABAergic and cholinergic systems, while molecular alterations include an increased expression of CAG trinucleotide at exon 1 from the huntingtin (htt) gene, as well as aggregation of mutant htt. Although several hypotheses regarding the mechanisms by which neurotoxicity is triggered in HD brains have been suggested on the basis of experimental evidence, so far it remains not clear which of them are predominant or whether they are complementary. Recent experimental evidence through transgenic mice models reveal an interesting interaction between expanded CAG triplets, mutant htt, and the increase in toxic metabolites from the kynurenine pathway. Further evidence supports the assumption that different toxic mechanisms (i.e. excitotoxicity, energy metabolism impairment, inflammatory events, oxidative stress, etc.) are confluent and depend on each other. In this review we will briefly summarize some of those findings and propose a final integrative hypothesis for HD.
NMDA receptor activation induces mitochondrial dysfunction, oxidative stress and apoptosis in cultured neonatal rat cardiomyocytes
Authors: Gao X , Xu X , Pang J , Zhang C , Ding JM , Peng X , Liu Y , Cao J-M
Published: October 2021

Glutamate is a well-characterized excitatory neurotransmitter in the central nervous system (CNS). Recently, glutamate receptors (GluRs) were also found in peripheral tissues, including the heart. However, the function of GluRs in peripheral organs remains poorly understood. In the present study, we found that N-methyl-D-aspartate (NMDA) could increase intracellular calcium ([Ca(2+)]i) level in a dose-dependent manner in cultured neonatal rat cardiomyocytes. NMDA at 10(-4) M increased the levels of reactive oxygen species (ROS), cytosolic cytochrome c (cyto c), and 17-kDa caspase-3, but depolarized mitochondrial membrane potential, leading to cardiomyocyte apoptosis. In addition, NMDA treatment induced an increase in bax mRNA but a decrease in bcl-2 mRNA expression in the cardiomyocytes. The above effects of NMDA were blocked by the NMDA receptor antagonist (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine hydrogen maleate (MK-801), and by ROS scavengers glutathione (GSH) and N-acetylcystein (NAC). These results suggest that stimulation of NMDA receptor in the cardiomyocyte may lead to apoptosis via a Ca(2+), ROS, and caspase-3 mediated pathway. These findings suggest that NMDA receptor may play an important role in myocardial pathogenesis.
Effect of monosodium glutamate on apoptosis and Bcl-2/Bax protein level in rat thymocyte culture
Authors: Pavlović V , Cekić S , Kocić G , Sokolović D , Živković V
Published: October 2021

Monosodium glutamate (MSG), the sodium salt of glutamate, is commonly used as a flavor enhancer in modern nutrition. Recent studies have shown the existence of glutamate receptors on lymphocytes, thymocytes and thymic stromal cells. In this study, we evaluated the in vitro effect of different MSG concentrations on rat thymocyte apoptosis and expression of two apoptosis-related proteins, Bcl-2 and Bax. Rat thymocytes, obtained from male Wistar rats, were exposed to increasing concentrations of MSG (ranging from 1 mM to 100 mM) for 24 h. Apoptosis was detected using the Annexin V-FITC/PI apoptosis detection kit and cells were analyzed using a flow cytometer. Expression of Bcl-2 and Bax proteins were determined with flow cytometry using respective monoclonal antibodies. Exposure to MSG resulted in a dose-dependent decrease in cell survival (as determined by trypan blue exclusion method). Annexin V-FITC/PI also confirmed that MSG increased, in a dose-dependent manner, apoptotic cell death in rat thymocyte cultures. MSG treatment induced downregulation of Bcl-2 protein, while Bax protein levels were not significantly changed. Our data showed that MSG significantly modulates thymocyte apoptosis rate in cultures. The temporal profile of Bcl-2 and Bax expression after MSG treatment suggests that downregulation of Bcl-2 protein and the resulting change of Bcl-2/Bax protein ratio may be an important event in thymocyte apoptosis triggered by MSG.
Increased insulin sensitivity in patients with anorexia nervosa: the role of adipocytokines
Authors: Dostálová I , Smitka K , Papežová H , Kvasničková H , Nedvídková J
Published: October 2021

Anorexia nervosa (AN) is characterized by self-induced starvation leading to severe weight and fat loss. In the present study, we measured fasting plasma levels of adiponectin, leptin, resistin, insulin and glucose in 10 women with a restrictive type of AN and in 12 healthy women (C). Insulin sensitivity was determined according to homeostasis model assessment of insulin resistance (HOMA-R). Plasma resistin, leptin and insulin levels were significantly decreased, whereas plasma adiponectin levels were significantly increased in patients with AN compared to the C. HOMA-R was significantly decreased in patients with AN compared to the C group. Plasma adiponectin and leptin concentrations negatively and positively correlated with the body mass index and percentage body fat in both groups. Plasma adiponectin levels were negatively related to plasma insulin levels in the AN group only. In conclusion, we demonstrated that AN is associated with significantly decreased plasma leptin and resistin levels, markedly increased plasma adiponectin levels and increased insulin sensitivity. Plasma leptin and adiponectin levels were related to the body size and adiposity. Hyperadiponectinemia could play a role in increased insulin sensitivity of patients with AN. Neither body size and adiposity nor insulin sensitivity are the major determinants of plasma resistin levels in AN.
Miniature excitatory synaptic ion currents in the earthworm Lumbricus terrestris body wall muscles
Authors: Volkov EM , Nurullin LF , Nikolsky E , Vyskočil F
Published: October 2021

The miniature excitatory postsynaptic currents (MEPCs) of the muscle cells of the earthworm Lumbricus terrestris were recorded by glass microelectrodes. In a single synaptic zone, three types of MEPC were recorded: a fast single-exponential type that decayed with tau =0.9 ms, a slow single-exponential with tau = 9.2 ms and a two-exponential MEPC with tau = 1.3 and 8.5 ms, respectively. The muscle cells of earthworms contain populations of yet-unidentified ionic channels that might be different from the common nicotinic and muscarinic groups of acetylcholine receptors, since these MEPCs are not sensitive to d-tubocurarine, atropine, benzohexonium or proserine. Alternatively, besides ACh receptors, the membrane may contain receptors for another yet-unidentified excitatory transmitter.
Nifedipine-sensitive vascular reactivity of femoral arteries in WKY: the effect of pertussis toxin pretreatment and endothelium removal
Authors: Líšková S , Kuneš J , Zicha J
Published: October 2021

Maintenance of norepinephrine (NE)-induced contraction is dependent on Ca(2+) influx through L-type voltage-dependent Ca(2+) channels (VDCC), which is opposed by nitric oxide. Adrenergic receptors are coupled with different G proteins, including inhibitory G proteins (Gi) that can be inactivated by pertussis toxin (PTX). Our study was aimed to investigate the effects of endothelium removal, PTX pretreatment and acute VDCC blockade by nifedipine on the contractions of femoral arteries stimulated by norepinephrine. We used 12-week-old male WKY, half of the rats being injected with PTX (10 microg/kg i.v., 48 h before the experiment), which considerably reduced their blood pressure (BP). Contractions of isolated arteries were measured using Mulvany-Halpern myograph. NE dose-response curves determined in femoral arteries from PTX-treated WKY rats were shifted to the right compared to those from control WKY. On the contrary, removal of endothelium augmented NE dose-response curves shifting them to the left. Acute VDCC blockade by nifedipine (10(-7) M) abolished all differences in NE dose-response curves which were dependent on the presence of either intact endothelium or functional Gi proteins because all NE dose-response curves were identical to the curve seen in vessels with intact endothelium from PTX-treated animals. We can conclude that BP reduction after PTX injection is accompanied by the attenuation of NE-induced contraction of femoral arteries irrespective of endothelium presence. Moreover, our data indicate that both vasodilator action of endothelium and Gi-dependent vasoconstrictor effect of norepinephrine operate via the control of Ca(2+) influx through VDCC.
The Use of Location-Based Augmented Reality as Information Media in The Digital Map Application of Jakabaring Sport City
Authors: Arifiani Siska , Manurung Miranda , Ginardi Raden Venantius Hari
Published: October 2021

Jakabaring Sport City (JSC) is an integrated sports facility complex and a public tourist spot in Palembang. Due to its extensive area and numerous facilities, visitors need a map with distinct markers for the landmark to make it easier when walking around. Recent technology advancements and its expanding role in various fields invites the question on how to integrate technology in the tourism industry. This journal aims to create an android-based digital map application with augmented reality (AR) as a guiding medium that will display directions towards the Jakabaring exit gate. This app is built using Maps SDK for Android to build the digital maps, Navigation SDK for Android to run navigation, and Maps for Unity to display AR objects that will show the directions towards the exit. The development focuses on a location-based service using GPS to track the user's whereabouts and provide real-time location data around the user. This application has a location search feature, a guiding direction (route and navigation) to a designed location, and an AR camera feature to display where the JSC exit gate is located. Every feature is working well and able to help users while visiting JSC. However, this application could still be improved in the AR feature because on its current version, it is only able to display directions to the JSC exit gate when users are within a radius of one kilometer or less to the exit.
Designing Motorcycle Security System Using E-KTP Sensor and SMS Gateway
Authors: Maulidin Juani Khuswara , Hiswara Abrar , Sari Rafika
Published: October 2021

Motorcycle demand is increasing as well as the cases of vehicle theft in Indonesia. Not only increase theft cases, number of new modes of theft of the motorcycle is also increase. According to the Statistics Supervisory Agency (BPS) data from 2015 to 2018, the data on the number of motorcycles is increasing, starting every year around 126,508,776 motorcycles are sold in Indonesia. In Indonesia, it is estimated that hundreds of motorbikes are stolen every month. How is the feasibility of motorcycle safety devices and the accuracy of the RFID module. Creating a motorcycle security system using a microcontroller-based RFID-RC522 module that can be read accurately and can be monitored in real time using received SMS and test the feasibility of a motorcycle safety device that can be monitored via cellphone. Making this tool using the Prototype method. The prototype is a development method from the previous system. The results of this research are in accordance with the analysis and design that has been made to get the result of an E-KTP-based motorcycle safety device to increase the security of personal items because this device can activate and deactivate motorcycles by using SMS. This tool can also help the community in maintaining security around the surrounding environment so that they can add a double security system to motorcycle vehicles.
Evaluation of Web-Based Car Park Information System at PPPSRS Puri Casablanca Jakarta
Authors: Sanusi Beno Aditya , Herlawati Herlawati , Kustanto Prio , Harviansyah Muhammad , Yana Anisa Feby , Kurniawan Ervan Dwi
Published: October 2021

Problems in conventional and manual parking are the inability to obtain complete, informative, and interactive parking information because the manual system cannot provide complete parking information. Information technology can be implemented in a parking system to help provide more accurate, fast, and easily accessible information. In addition, the parking system interface can be made user-friendly, hence, the parking attendants can operate it properly. This parking system uses a client-server model with a shared database. The author found problems in the car parking system at PPPSRS Puri Casablanca Jakarta, i.e. still using the previous version of XAMPP. The research methodology used in this study are: Observation Methods, Literature Methods, and Interview Methods. The proposed Car Parking application showed acceptable for use by parking attendants of PPPSRS Puri Casablanca Apartment Jakarta. The menu choices presented are sufficient and the web design is simple so it doesn't confuse the parking attendant.
English-Language Periodicals in Parisian Reading Rooms and the Cross-Channel Transfer of Editorial Innovation (1800–65)
Authors: Cooper-Richet Diana
Published: October 2021

In the historical context of the development and modernization of the press, of an increasingly intense transnational circulation of ideas and of editorial styles, this essay sets out to analyze the reasons why reading rooms specialized in the foreign-language press, especially in English—for which the market was narrow—were successful in Paris during the first half of the nineteenth century. It examines the consequences of the circulation of the normally difficult to access British periodicals and newspapers, such as the Edinburgh Review, the Quarterly Review and the Westminster Review present in these reading rooms, on the transformation of the French media system. In the 1850s and 1860s, the wind started to change direction. By then, on the other side of the Channel, Alexander Macmillan and Mathew Arnold had become fervent admirers of the famous Revue des deux mondes. This turnabout testifies to the complexity of the mechanisms at work behind transnational cultural transfers and media innovation in France and in Britain at the time.
‘Essentially an American Institution Planted on Foreign Soil’: The American Library in Paris, the Paris Herald, the Paris Tribune and Ex Libris
Authors: Cannon Nissa Ren
Published: October 2021

In 1920, the American Library in Paris (ALP) was incorporated, with the desire to ‘be a somewhat adequate representation of American life and thought’ in the city. This paper will argue that the ALP - an institution established for overseas soldiers in 1918, which became its own entity in 1920 and celebrated a century of service in 2020 - would do more than represent America in the interwar period: it would play a role in shaping American identity as well.

Through archival materials, this paper explores the ALP’s representation in the three periodicals most imbricated with its interwar existence: the Paris editions of the Chicago Tribune and the New York Herald, and the little magazine, Ex Libris. I argue that the ALP - in both its physical and psychic forms - was an important site for the formation of transnational American identity in the interwar period, and that it strived to weigh in on conversations about emerging literary movements, including modernism and the Harlem Renaissance. American identity, as the Library represented it, combined national exceptionalism with a true desire for transnational cooperation. It was firmly at home on international soil, and well-versed in the era’s literary debates.
English-Language Periodicals and Reading Rooms in Nineteenth-Century Italy as Spaces of Intercultural Contact and Exchange
Authors: Richet Isabelle
Published: October 2021

This paper discusses the symbiotic relationship that developed between English-language periodicals published in Italy and major reading rooms in Rome and Florence. This relationship took various configurations – from Luigi Piale in Rome, who opened a reading room and published the weekly The Roman Advertiser, to the Gabinetto Vieusseux in Florence that provided access to the many English-language periodicals published in Italy – and created important spaces of transnational cultural interaction. The paper looks at the cultural practices and the forms of sociability represented by the reading of periodicals and the patronizing of reading rooms as ‘imported traditions’ brought to Italy by the many British cultured travellers and residents in the nineteenth century. It identifies the actors who promoted these cultural practices (editors, librarians, cosmopolitan intellectuals) and analyses their role as mediating figures who created in-between spaces where cross-cultural exchanges unfolded. The paper also discusses the broader transnational cultural dynamic at work as those cultural practices imported from England favoured a greater engagement of British visitors and expatriates with the Italian political and cultural environment.
Crossing the Atlantic: Reading Rooms and Foreign-Language Periodicals in Nineteenth-Century Rio de Janeiro
Authors: Guardini Vasconcelos Sandra
Published: October 2021

Reading rooms, a totally unprecedented kind of institution in a country newly independent from Portuguese rule, started being set up in nineteenth-century Rio de Janeiro in the late 1820s. Catering mainly for the foreign communities, their fare consisted for the most part in imported printed material. Although it might sound like an exaggeration to claim that Rio was flooded with newspapers, books and periodicals, they became gradually and increasingly available to its inhabitants. The establishment of the British Subscription Library in 1826, the Portuguese Circulating Library in 1837, and the Biblioteca Fluminense in 1847 played a pivotal role in the circulation of British and French periodicals, allowing for the dissemination of news, ideas, key political, social and economic issues, as well as the diffusion of fiction and literary news. This essay reveals the presence and circulation of some of the foreign periodicals in nineteenth-century Rio de Janeiro libraries and reading rooms and traces their impact on the local Republic of Letters.
Control of Ship Roll and Yaw Angles During Turning Motion
Authors: GÖKSU Burak , BAYRAMOĞLU Kubilay
Published: October 2021

The aim of this paper is Ships are exposed to assorted hydrodynamic forces originating from internal and external influences. The adverse effect of rolling caused by turning motion should be reduced with anti-rolling systems, to ensure safe navigation. If this effect is not eliminated, it may prevent the ship from keeping its course safely. In this study, fin stabilizer system the computational analysis of the roll motion formed by the turning motion and wave effect is included in fin stabilizer system. The rudder motion that allows the ship to have the desired maneuvering angle; calculations of the fin system, which decreases the excessive roll moment, and the roll motion caused by these two effects are examined using MATLAB software. The analysis period has been determined as 300 seconds, which is the time to reach the desired turning angle of the ship. Linear quadratic tracking (LQT) algorithm has been used to solve partially unknown continuous-time problems such as roll motion in this study.
AUTOMATION OF CONTROL OF OPTICAL PARAMETERS OF IMAGE CONVERTER TUBES
Authors: Kvitovskiy Oleg A. , Nikulin Dmitry M.
Published: October 2021

The article proposes a scheme of an installation with a digital camera for automating the measurement viewing area picture quality and the output brightness non-uniformity ratio of an image converter tubes. The requirements for the CMOS-matrix and the lens for the digital camera used are formulated.
IMPROVING VIDEO CONFERENCE SECURITY WITH ARTIFICIAL INTELLIGENCE
Authors: Kizin Vasily S. , Novikov Sergei N.
Published: October 2021

As virtual workspaces become more sophisticated, the challenge of protecting an organization's sensitive data from accidental or intentional disclosure and compliance is becoming more challenging. With AI-powered security solutions specifically designed to monitor collaboration platforms, organizations can identify and respond to risks in real time to protect their data and that of their employees.
DESIGN STAGE IN THE LIFE CYCLE OF AN OPTICAL DEVICE
Authors: Lepen Alexandr U.
Published: October 2021

Any product, including optical devices, during its existence goes through a series of states from the idea of creation to disposal, which is called the product life cycle. Design is a complex and creative process of a specialist (designer), invariant to various types and complexity of devices. Designing requires the designer to in addition to special, subject-specific knowledge, as well as knowledge of the methodology, tools and rules for the implementation of project procedures. Modern design of optical devices is carried out in a software environment, the so-called the system of information support of the product life cycle, which makes it necessary for students to master the appropriate software.
OCCURRENCE OF DEFECTS THE PRODUCTION OF MASKS
Authors: Iisenko Anastasiya V. , Nikulin Dmitry M.
Published: October 2021

The article discusses the main stages of the route for the production of masks, during which defects are formed. The main defects and possible reasons for their appearance are given. A method of correcting defects in the form of punctures and undergrowth by the laser method is considered. The disadvantages of the laser method in correcting the elements of the topological pattern of masks are presented.
ORGANIZATION OF WORKS ON METROLOGICAL SUPPORT IN STRUCTURAL DIVISIONS OF THE ENTERPRISE
Authors: Bazaka Alexandra A.
Published: October 2021

The article discusses a set of scientific and organizational - technical measures for metrological support in the structural units of radio technical flight support. The proposed set of measures aimed at increasing the reliability of control of the measured parameters, maintaining the uniformity and required accuracy of measurements, ensuring continuous availability and safety of flights, as well as reliable air traffic control.
MULTI-LEVEL ARCHITECTURE OF KNOWLEDGE MANAGEMENT SYSTEM TO IMPROVE INFORMATION SECURITY LEVEL
Authors: Mongush Amyrtaa K. , Karmanov Igor N.
Published: October 2021

Information security is a major concern because security risks can greatly affect the assets of an organization. To increase the level of information security, it is proposed to use a knowledge management system with a multi-level architecture in order to improve knowledge exchange and facilitate decision-making, as well as reduce dependence on individual information security experts.
DEVELOPMENT OF A MODEL OF AN AUGMENTED REALITY INFORMATION SYSTEM WITH GEO-LINKED DATA
Authors: Safin Renat M. , Bugakov Petr Yu.
Published: October 2021

The article describes the sequence of works for creation of an augmented reality system with geo-linked data. The criteria for selecting software for creation of a system are formulated, and the main tools that meet these criteria are indicated. The architectural components of the system and the principles of functioning of the augmented reality system model with geo-linked data are described. It will help expand the scope of electronic maps and simplify their use.
OPERATING PRINCIPLE OF THE DEVICE FOR MEASURING THE GEOMETRIC CHARACTERISTICS OF THE GUN BARREL
Authors: Taranova Ludmila S. , Mikhailov Igor O.
Published: October 2021

The article presents the principle of operation of the measuring device, which is based on an internal gauge that moves along the barrel bore using a movement system fixed on the end surface. The article presents the principle of operation of the optoelectronic module, which is designed for contact measurement of deviations of linear dimensions from the reference ones. This measuring device is multifunctional, and is designed not only to determine the diameter of the barrel in many sections, but also to construct its profile, taking into account the curvature of the channel.
ANALYSIS OF A PROJECT THE NATIONAL TRACEABILITY CHAIN FOR INSTRUMENTS MEASURING MASS AND VOLUME OF LIQUID IN A FLOW
Authors: Shakalov Konstantin A. , Vikhareva Nadezhda A.
Published: October 2021

The article provides a comparative analysis of the canceled, current and draft new version of the state verification scheme for measuring the mass and volume of liquid in the flow. The prerequisites and reasons for making changes are considered, as well as the estimated consequences of the changes made for the metrological services using standards and instruments for measuring the mass and volume of liquid in the flow.
OPTICAL SIGHTS WITH INTERCHANGEABLE MAGNIFICATION
Authors: Shmelev Evgeniy V. , Khatsevich Tatiana N.
Published: October 2021

The article notes the development trend of optical sights made with a discrete change in magnification. Using two optical schemes in ideal components as an example, we consider the possibility of deviating from the classical approaches to the implementation of a discrete change in magnification to improve the performance of devices relative to existing analogs.
Proteomic and Biological Analysis of the Effects of Metformin Senomorphics on the Mesenchymal Stromal Cells
Authors: Acar Mustafa Burak , Ayaz-Güner Şerife , Gunaydin Zeynep , Karakukcu Musa , Peluso Gianfranco , Di Bernardo Giovanni , Özcan Servet , Galderisi Umberto
Published: October 2021

Senotherapeutics are new drugs that can modulate senescence phenomena within tissues and reduce the onset of age-related pathologies. Senotherapeutics are divided into senolytics and senomorphics. The senolytics selectively kill senescent cells, while the senomorphics delay or block the onset of senescence. Metformin has been used to treat diabetes for several decades. Recently, it has been proposed that metformin may have anti-aging properties as it prevents DNA damage and inflammation. We evaluated the senomorphic effect of 6 weeks of therapeutic metformin treatment on the biology of human adipose mesenchymal stromal cells (MSCs). The study was combined with a proteome analysis of changes occurring in MSCs’ intracellular and secretome protein composition in order to identify molecular pathways associated with the observed biological phenomena. The metformin reduced the replicative senescence and cell death phenomena associated with prolonged in vitro cultivation. The continuous metformin supplementation delayed and/or reduced the impairment of MSC functions as evidenced by the presence of three specific pathways in metformin-treated samples: 1) the alpha-adrenergic signaling, which contributes to regulation of MSCs physiological secretory activity, 2) the signaling pathway associated with MSCs detoxification activity, and 3) the aspartate degradation pathway for optimal energy production. The senomorphic function of metformin seemed related to its reactive oxygen species (ROS) scavenging activity. In metformin-treated samples, the CEBPA, TP53 and USF1 transcription factors appeared to be involved in the regulation of several factors (SOD1, SOD2, CAT, GLRX, GSTP1) blocking ROS.
Combined Chaetocin/Trichostatin A Treatment Improves the Epigenetic Modification and Developmental Competence of Porcine Somatic Cell Nuclear Transfer Embryos
Authors: Jeong Pil-Soo , Yang Hae-Jun , Park Soo-Hyun , Gwon Min Ah , Joo Ye Eun , Kim Min Ju , Kang Hyo-Gu , Lee Sanghoon , Park Young-Ho , Song Bong-Seok , Kim Sun-Uk , Koo Deog-Bon , Sim Bo-Woong
Published: October 2021

Developmental defects in somatic cell nuclear transfer (SCNT) embryos are principally attributable to incomplete epigenetic reprogramming. Small-molecule inhibitors such as histone methyltransferase inhibitors (HMTi) and histone deacetylase inhibitors (HDACi) have been used to improve reprogramming efficiency of SCNT embryos. However, their possible synergistic effect on epigenetic reprogramming has not been studied. In this study, we explored whether combined treatment with an HMTi (chaetocin) and an HDACi (trichostatin A; TSA) synergistically enhanced epigenetic reprogramming and the developmental competence of porcine SCNT embryos. Chaetocin, TSA, and the combination significantly increased the cleavage and blastocyst formation rate, hatching/hatched blastocyst rate, and cell numbers and survival rate compared to control embryos. In particular, the combined treatment improved the rate of development to blastocysts more so than chaetocin or TSA alone. TSA and combined chaetocin/TSA significantly reduced the H3K9me3 levels and increased the H3K9ac levels in SCNT embryos, although chaetocin alone significantly reduced only the H3K9me3 levels. Moreover, these inhibitors also decreased global DNA methylation in SCNT embryos. In addition, the expression of zygotic genome activation- and imprinting-related genes was increased by chaetocin or TSA, and more so by the combination, to levels similar to those of in vitro-fertilized embryos. These results suggest that combined chaetocin/TSA have synergistic effects on improving the developmental competences by regulating epigenetic reprogramming and correcting developmental potential-related gene expression in porcine SCNT embryos. Therefore, these strategies may contribute to the generation of transgenic pigs for biomedical research.
Identification of the Prognostic Signature Associated With Tumor Immune Microenvironment of Uterine Corpus Endometrial Carcinoma Based on Ferroptosis-Related Genes
Authors: Liu Jinhui , Wang Yichun , Meng Huangyang , Yin Yin , Zhu Hongjun , Ni Tingting
Published: October 2021

Background: Uterine corpus endometrial carcinoma (UCEC) is the sixth most common cancer worldwide. Ferroptosis plays an important role in malignant tumors. However, the study of ferroptosis in the endometrial carcinoma remains blank.

Methods: First, we constructed a ferroptosis-related signature based on the expression profiles from The Cancer Genome Atlas database. Then, patients were divided into the high-risk and low-risk groups based on this signature. The signature was evaluated by Kaplan–Meier analysis and receiver operating characteristic (ROC) analysis. We further investigated the relationship between this signature and immune microenvironment via CIBERSORT algorithm, ImmuCellAI, MAF, MSI sensor algorithm, GSEA, and GDSC.

Results: This signature could be an independent prognostic factor based on multivariate Cox regression analysis. GSEA revealed that this signature was associated with immune-related phenotype. In addition, we indicated the different status of immune infiltration and response to the immune checkpoint between low-risk and high-risk groups. Patients in the low-risk group were more likely to present with a higher expression of immune checkpoint molecules and tumor mutation burden. Meanwhile, the low-risk patients showed sensitive responses to chemotherapy drugs.

Conclusion: In summary, the six ferroptosis-related genes signature could be used in molecular subgrouping and accurately predict the prognosis of UCEC.
A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation
Authors: Shen Xiaoxu , Wei Yuanhang , Liu Wei , You Guishuang , Tang Shuyue , Su Zhenyu , Du Mingxin , He Jian , Zhao Jing , Tian Yongtong , Zhang Yao , Ma Menggen , Zhu Qing , Yin Huadong
Published: October 2021

Circular RNA (circRNA) is a class of endogenous non-coding RNAs without 5′ and 3′ ends; an increasing number of studies show that circRNA is involved in skeletal muscle development. From our previous sequencing data, the circRNAome in breast muscle of two chicken lines with a distinct rate of muscle development, which included a fast muscle growing broiler (FMGB) and a slow muscle growing layer (SMGL), we found a novel differentially expressed circRNA generated by intersectin 2 (ITSN2) gene (named circITSN2). We verified that circITSN2 is a skeletal muscle-enriched circRNA that promotes chicken primary myoblast (CPM) proliferation and differentiation. Further molecular mechanism analysis of circITSN2 in chicken myogenesis was performed, and we found circITSN2 directly targeting miR-218-5p. Besides, miR-218-5p inhibits CPM proliferation and differentiation, which is contrary to circITSN2. Commonly, circRNAs act as a miRNA sponge to alleviate the inhibition of miRNAs on mRNAs. Thus, we also identified that a downstream gene LIM domain 7 (LMO7) was inhibited by miR-218-5p, while circITSN2 could block the inhibitory effect of miR-218-5p by targeting it. Functional analysis revealed that LMO7 also accelerates CPM proliferation and differentiation, which was similar to circITSN2 but contrary to miR-218-5p. Taken together, these results suggested that circITSN2 promotes chicken embryonic skeletal muscle development via relieving the inhibition of miR-218-5p on LMO7. Our findings revealed a novel circITSN2/miR-218-5p/LMO7 axis in chicken embryonic skeletal muscle development, which expands our understanding of the complex muscle development regulatory network.
Novel Strategies in C-H Oxidations for Natural Product Diversification—A Remote Functionalization Application Summary
Authors: Junrong Huang , Min Yang , Chuan Dai , Yajun Zhou , Huilong Fang , Lizhi Zhu , Feng Yin , Zigang Li
Published: October 2021

Selectively activating the distal inactive C-H bond for functionalization is one of the on-going challenge in organic synthetic chemistry. In recent years, benefiting from the development of selective synthesis methods, novel methodologies not only make it possible to break non-traditional chemical bonds and attain more diversity in inactive sites, but also provide more possibilities for the diversification of complex natural products. Direct C-H bond functionalization approaches make it feasible to explore structure-activity relationship (SAR), generate metabolites and derivatives, and prepare biological probes. Among them, direct oxidation of inert C-H bonds is one of the most common methods for natural product diversification. In this review, we focus on the application of remote functionalization of inert C-H bonds for natural products derivatization, including the establishment of oxidation methods, the regulation of reaction sites, and the biological activities of derivatives. We highlight the challenges and opportunities of remote functionalization of inert C-H bonds for natural product diversification through selected and representative examples. We try to show that inert C-H bond oxidation, properly regulated and optimized, can be a powerful and efficient strategy in both synthetic and medicinal chemistry.
Combined the Photocatalysis and Fenton-like Reaction to Efficiently Remove Sulfadiazine in Water Using g-C3N4/Ag/γ-FeOOH: Insights Into the Degradation Pathway From Density Functional Theory
Authors: Zhu Yangchen , Zhao Furong , Wang Fei , Zhou Beihai , Chen Huilun , Yuan Rongfang , Liu Yuxin , Chen Yuefang
Published: October 2021

Sulfadiazine (SDZ) is a common antibiotic pollutant in wastewater. Given that it poses a risk as an environmental pollutant, finding effective ways to treat it is important. In this paper, the composite catalytic material g-C3N4/Ag/γ-FeOOH was prepared, and its degradation performance was studied. g-C3N4/Ag/γ-FeOOH had a superior degradation effect on SDZ than g-C3N4 and γ-FeOOH. Compared with different g-C3N4 loadings and different catalyst dosages (5, 10, 25, and 50 mg/L), 2 mg/L g-C3N4/Ag/γ-FeOOH with a g-C3N4 loading of 5.0 wt% has the highest degradation promotion rate for SDZ, reaching up to 258.75% at 600 min. In addition, the photocatalytic enhancement mechanism of the catalyst was studied. Density functional theory (DFT) calculations indicated that the enhancement of photocatalytic activity was related to the narrowing of the forbidden band and the local electron density of the valence band. The bandgap of the catalyst was gradually narrowed from 2.7 to 1.05 eV, which can increase the light absorption intensity and expand the absorption edge. The density of states diagram showed that the local resonance at the interface could effectively improve the separation efficiency of e−-h+ pairs. Four degradation paths of SDZ were speculated based on DFT calculations. The analysis confirmed that the degradation path of SDZ primarily included Smiles-type rearrangement, SO2 extrusion, and S-N bond cleavage processes.
Assessment of the Ability of CMIP6 GCMS to Simulate the Boreal Summer Intraseasonal Oscillation Over Southeast Asia
Authors: Abatan Abayomi A. , Collins Matthew , Babel Mukand S. , Khadka Dibesh , De Silva Yenushi K.
Published: October 2021

The boreal summer intraseasonal oscillation (BSISO) plays an important role in the intraseasonal variability of a wide range of weather and climate phenomena across the region modulated by the Asian summer monsoon system. This study evaluates the strengths and weaknesses of 19 Coupled Model Intercomparison Project Phase 6 (CMIP6) models to reproduce the basic characteristics of BSISO. The models' rainfall and largescale climates are evaluated against GPCP and ERA5 reanalysis datasets. All models exhibit intraseasonal variance of 30–60-day bandpass-filtered rainfall and convection anomalies but with diverse magnitude when compared with observations. The CMIP6 models capture the structure of the eastward/northward propagating BSISO at wavenumbers 1 and 2 but struggle with the intensity and location of the convection signal. Nevertheless, the models show a good ability to simulate the power spectrum and coherence squared of the principal components of the combined empirical orthogonal function (CEOF) and can capture the distinction between the CEOF modes and red noise. Also, the result shows that some CMIP6 models can capture the coherent intraseasonal propagating features of the BSISO as indicated by the Hovmöller diagram. The contribution of latent static energy to the relationship between the moist static energy and intraseasonal rainfall over Southeast Asia is also simulated by the selected models, albeit the signals are weak. Taking together, some of the CMIP6 models can represent the summertime climate and intraseasonal variability over the study region, and can also simulate the propagating features of BSISO, but biases still exist.
HATCH Score and Left Atrial Size Predict Atrial High-Rate Episodes in Patients With Cardiac Implantable Electronic Devices
Authors: Chen Ju-Yi , Chen Tse-Wei , Lu Wei-Da
Published: October 2021

Background: Patients with sustained atrial high-rate episodes (AHRE) have a high risk of major adverse cardio/cerebrovascular events (MACCE). However, the prediction model and factors for the occurrence of AHRE are unknown. We aimed to identify independent factors and various risk models for predicting MACCE and AHRE.

Methods: We retrospectively enrolled 314 consecutive patients who had cardiac implantable electronic devices (CIEDs). The primary endpoint was MACCE after AHRE ≥3, 6 min, and 6 h. Atrial high-rate episodes was defined as >175 bpm (Medtronic®) lasting ≥30 s. Multivariate Cox and logistic regression analysis with time-dependent covariates were used to determine variables associated with independent risk of MACCE and occurrence of AHRE ≥3 min, respectively.

Results: One hundred twenty-five patients (39.8%) developed AHRE ≥3 min, 103 (32.8%) ≥6 min, and 55 (17.5%) ≥6 h. During follow-up (median 32 months), 77 MACCE occurred (incidence 9.20/100 patient years, 95% CI 5.66–18.39). The optimal AHRE cutoff value was 3 min for MACCE, with highest Youden index 1.350 (AUC, 0.716; 95% CI, 0.638–0.793; p < 0.001). Atrial high-rate episodes ≥3 min−6 h were independently associated with MACCE. HATCH score and left atrial diameter were independently associated with AHRE ≥3 min. The optimal cutoff for HATCH score was 3 and for left atrial diameter was 4 cm for AHRE ≥3 min.

Conclusion: Patients with CIEDs who develop AHRE ≥3 min have an independently increased risk of MACCE. Comprehensive assessment using HATCH score and echocardiography of patients with CIEDs is warranted.