Peculiaritie of Distribution of Polymorphic Variants of IL1Β Gene in Patients with Atherosclerosis and Metabolic Syndrome
Authors: Vladimirovna Saranchina Y. , Aleksandrovna Rossova N. , Vladimirovich Khanarin N. , Yur'evna Kilina O. , Vyacheslavovna Dutova S. , Sergeevna Kulakova T. 
Published: April 2019

Objective:

The purpose of the study was to analyze the association of allelic
polymorphism of IL1В gene C>T loci -31 and +3953 with atherosclerotic changes of
artries in patients with Metabolic Syndrome (MS).

Materials and Methods:

The main group of the study included 30 consecutive patients
(24 women and 6 men, mean age - 51.7±2.2 years), for examination and treatment in the
therapeutic Department of the Republican clinical hospital named "G. YA. Remishevskaya"
(Abakan) about arterial hypertension or suspicion of type 2 diabetes. The criteria for inclusion
in the core group included: compliance with the MS criteria according to the IDF criteria
(2006); and the presence of ultrasound markers of Atherosclerosis (AS) according to
the study of brachiocephalic arteries (presence of Atherosclerotic Plaques (ASP) and
stenosis ≥30%). The control group included persons who underwent a planned medical
examination in the Republican clinical hospital name "G. YA. Remishevskaya" (Abakan).
A total of 35 patients (26 women and 9 men, mean age 44.7±1.5 years) were selected. The
study involved the Russian population (Caucasians) living in the territory of the Republic
of Khakassia. All the necessary examination and data collection were conducted including
anamnestic data, anthropometric examination (measurements of length and body mass,
waist circumference) body mass index, laboratory examination of blood biochemical parameters
(glucose and lipid) and instrumental examination (blood pressure measurement,
conducting ECG and ultrasound the brachiocephalic arteries). Single-nucleotide polymorphisms
(SNP) of the promoter region of the IL1B gene at position-31C/T (rs1143627) and
polymorphism in the coding part of the gene in exon 5 +3953C/T (rs 1143634) were studied
by restriction analysis of amplification products (RFLP analysis).

Results:

The risk of development of AS in patients with MS may be higher in carriers of
genotype TT (OR = 1,76; 95% CI: (0,96-3,24)) or T allele (OR = 1,44; 95% CI: (0,82-
2,53)) IL1В gene in the polymorphic locus of the T-31С and genotype CT (OR = 1,85;
95% CI: (0,92-3,37)) or T allele (OR = 1,35; 95% CI: (0,63-2,89)) IL1В gene in the
polymorphic locus of C + 3953T. The most common combination of gene polymorphisms
IL1В was haplotype (-31) ТC/(+3953)СС in both the groups surveyed (40.6% to 36.8%,
respectively). Variant (-31)TT/(+3953)CT in the main group was found significantly more
often (15.8%, at χ2= 4.92, at p=0.03) than in the control group (3.1 %). The value of the
odds ratio in this case was 3.99 (95% CI: (1.08-14.79), which indicates the risk of AS development
against the background of MS in carriers of combined genotype inheritance
(-31)TT/(+3953) CT.

Conclusion:

The risk of development of AS in the background of MS is increased in
carriers of combinations of SNPs (-31)TT/(+3953)CT IL1В gene responsible for hyperproduction
of this cytokine. In this connection, further studies of the association of genes
with MS and AS components should focus on intergenic interactions.