bluuebunny/crossref_metadata_2025 huggingface dataset:
CONTRIBUIÇÕES DE UM PROGRAMA DE MENTORIA PARA O DESENVOLVIMENTO PROFISSIONAL DE PROFESSORAS INICIANTES DO ENSINO FUNDAMENTAL – ANOS INICIAIS
Authors: Pinheiro Tarciana dos Santos , De Souza Ana Paula Gestoso
Published: June 2022

Esta pesquisa é de caráter qualitativo descritivo-analítico tendo um ambiente natural como fonte direta de dados, estes são descritivos e a maior preocupação se concentra no processo, com a complexidade das trajetórias dos participantes, ou seja, o significado que as pessoas dão às coisas e à sua vida é objeto de atenção do pesquisador. Analisa a trajetória formativa percorrida por duas professoras iniciantes, atuantes no ensino fundamental –anos iniciais, ao se inserirem em um programa de acompanhamento e apoio ao iniciante, intitulado mentoria. No programa em questão um professor experiente (mentor) auxilia professores iniciantes a desenvolver competências profissionais mais rapidamente e na fundamentação de novas práticas profissionais.  A partir da análise das trajetórias profissionais dessas duas iniciantes compartilhadas em um ambiente virtual de aprendizagem foi possível delinear tensões e aprendizagens que envolvem o percurso inicial de seu desenvolvimento profissional. A pesquisa evidenciou a importância de uma formação contínua, e, assim, traduz a essencial relevância dos programas de indução, uma vez que percebe-se um dos fatores indispensáveis ao desenvolvimento profissional docente: o acompanhamento desses profissionais no início da carreira ameniza as tensões, condiciona novas perspectivas, providencia quebra de paradigmas, amplia os repertórios teóricos/metodológicos dos(as) professores(as). Assim, o programa de mentoria pode ser visto como uma oportunidade e experiência planejada de forma sistemática com o intuito de propiciar que o iniciante se envolva em um movimento de questionamento das situações que vivencia na prática docente e a construção de planos de ação para superação das tensões.
Apresentação
Authors: Mezzomo Frank Antônio , Hahn Fabio André
Published: June 2022

Apresentação
Projetos desenvolvidos em 2008/2009
Authors: Pátaro Cristina Satiê de Oliveira
Published: June 2022

Projetos desenvolvidos em 2008/2009
Projetos desenvolvidos em 2007/2008
Authors: Mezzomo Frank Antônio
Published: June 2022

Projetos desenvolvidos em 2007/2008
Gestos de pesquisa: para depois de amanhã, hoje
Authors: Aleixo Antonio Carlos
Published: June 2022

O texto, ancorado no gênero ensaio, socorre-se, em grande parte, na subjetividade, para traçar um ponto de vista sobre as condições históricas do aparecimento e desenvolvimento do Núcleo de Pesquisas Multidisciplinares (NUPEM), da Faculdade Estadual de Ciências e Letras de Campo Mourão. Procura expor as condições internas da história recente da instituição, especialmente nos últimos dez anos, e tenta localizar a função que o NUPEM vem desempenhando na sua proposta diretriz. Neste caso, são referenciados tanto os atores quanto os objetivos e os desdobramentos de cada período, desde 2007 até 2009.
Rememoração – 10 anos do NUPEM: lembranças e esquecimentos
Authors: Paula Zueleide Casagrande de
Published: June 2022

O presente artigo está caracterizado como artigo-depoimento, tem o sentido memorialístico acerca da construção da história e da memória da Faculdade Estadual de Ciências e Letras de Campo Mourão. Foi elaborado a partir de uma posição político-social da autora, com base em sua memória e com suportes teórico-explicativos pautados nas obras de Michel Foucault, Jacques Le Goff, Ecléia Bosi e Salles Oliveira entre outros inspiradores de ações sócio políticas. Visa apontar a relevância da Iniciação Científica e da Pesquisa para todo o Ensino Superior no País, incluindo neste universo, as instituições desvinculadas do apoio político-econômico concedidos às universidades, como as faculdades. Procura abordar formas de resistências e de lutas que culminaram com a transformação da FECILCAM de faculdade horista à instituição produtora de pesquisa e responsável por seu lugar político de instituição socialmente transformadora da região em que está localizada.
v. 2, n. 2 (2010)
Authors: Mezzomo Frank Antônio
Published: June 2022

Expediente e Sumário
Dificuldades na gestão de negócios: resultados de uma pesquisa de campo junto aos empresários de Campo Mourão via entrevista estruturada
Authors: Bastos Tiago , Crepaldi Jesus , Dias Ricardina , Jesus Marcos Junio Ferreira de , Bastos Luciana Aparecida
Published: June 2022

Este artigo consiste em apresentar os resultados de uma pesquisa de campo junto aos empresários de Campo Mourão, visando detectar as principais dificuldades que os mesmos encontram em seu dia-a-dia na gestão de seus negócios. O trabalho foi realizado utilizando-se da aplicação de questionários aos empresários e, suas respostas, foram analisadas pelo método da estatística descritiva, o qual, mediante seu instrumental técnico, respalda as conclusões contidas neste trabalho.
O planejamento regional da costa oeste do Paraná é integrado?
Authors: Gemeli Vanderleia , Souza Edson Belo Clemente de
Published: June 2022

Este estudo busca analisar a (des)integração do planejamento regional dentre os municípios lindeiros ao Lago de Itaipu (Foz do Iguaçu, Santa Terezinha de Itaipu, São Miguel do Iguaçu, Missal, Santa Helena, Diamante do Oeste, Entre Rios do Oeste, Pato Bragado, Marechal Cândido Rondon, Mercedes, Guaíra, Terra Roxa, Medianeira, Itaipulândia e São José das Palmeiras). Sendo a concepção regional uma realidade entre esses municípios, o fluxo de pessoas, de serviços e de mercadorias densifica a relação entre eles, implicando políticas que os articulem. A partir do momento em que esses municípios se encontram inseridos em uma mesma realidade e numa mesma dinâmica de desenvolvimento, tornase fundamental analisar o papel do planejamento para melhor compreender a região em estudo. A percepção da integração ou da falta dela no planejamento regional se fará pela análise dos Planos Diretores desses municípios, da atuação de instituições públicas e privadas e dos programas implantados por essas instituições.
Inserção externa e desenvolvimento econômico: um estudo das experiências brasileira e chinesa no período 1990-2008
Authors: Araújo Eliane , Araújo Elizangela Luzia
Published: June 2022

Este artigo analisa os padrões de desenvolvimento de dois países – Brasil e China – entre 1980 e 2008. É discutida a diferença de estratégias entre as duas economias, destacando-se o sucesso da estratégia chinesa pautada no forte incentivo ao setor exportador, na condução independente de políticas econômicas, sobretudo a administração do câmbio, que lograram ao país alcançar altas taxas de crescimento nas últimas décadas, assim como a condição de grande competidor mundial. Ao contrário, o Brasil, ao optar pela adoção das propostas do “Consenso de Washington”, implementa sua estratégia de estabilização cuja combinação de políticas - taxas de juros elevadas e sobrevalorização cambial - têm causado um forte viés anti-exportador e pró-importação, com o desestímulo aos investimentos produtivos, com sérias conseqüências para a economia brasileira.
Avaliação do desempenho comercial do Mercosul: 1994-2005
Authors: Bastos Luciana Aparecida
Published: June 2022

Avaliação do desempenho comercial do Mercosul: 1994-2005
Diferenças de gênero na juventude: um estudo a partir das vivências de estudantes de ensino médio do município de Campo Mourão
Authors: Luz Lucimar da , Pátaro Cristina Satiê de Oliveira
Published: June 2022

Diferenças de gênero na juventude: um estudo a partir das vivências de estudantes de ensino médio do município de Campo Mourão
Organização, catalogação e descrição dos autos da vara civil da comarca de Campo Mourão (1972-1974)
Authors: Ferro Fernanda Cristina , Mezzomo Frank Antônio
Published: June 2022

Organização, catalogação e descrição dos autos da vara civil da comarca de Campo Mourão (1972-1974)
Hermenêutica e psicanálise: disparidades e complementaridades na interpretação
Authors: Santos Isabela Karina , Hellmann Risolete Maria
Published: June 2022

Este artigo refere-se ao estudo das complementaridades e disparidades entre a interpretação na psicanálise e na hermenêutica. Tomou-se, como ponto de partida, o pensamento de Freud, Lacan, Schleiermacher e Gadamer, entre outros. Os resultados revelam que a hermenêutica e a psicanálise convergem na sua concepção compreensiva na metodologia de pesquisa, valem-se, metodologicamente, da interpretação dos significados da linguagem (essência das pesquisas qualitativas), e buscam compreender o modo como os fenômenos se manifestam por meio da linguagem verbal ou não-verbal, levando em conta a subjetividade humana. Quanto às suas disparidades, pontuam-se: a perspectiva de mundo da mudança radical que a psicanálise se encontra e, por outro lado, a manutenção do status quo da hermenêutica, à natureza dos fenômenos de que tratam e as diferenças nas formas de interpretar.
Sobre o poder da imagem plástica: quando a dessemelhança muda os rumos da representação
Authors: Santos Jefferson Fernando Voss dos
Published: June 2022

Trilhando os percursos analíticos vivenciados pela Análise de Discurso francesa e seus desdobramentos a partir do método arqueológico de Michel Foucault, o texto que segue procura argumentar a favor da eficácia da imagem plástica em corroborar na ruptura ou permanência da memória social a partir dos elementos linguísticos que integra e de sua função/disfunção analógica. Para tanto, recuperaremos a problemática foucaultiana da representação em seus níveis de semelhança e similitude e, por fim, analisaremos um texto imagético a fim de demonstrar esse funcionamento discursivo. Os resultados mostram que a imagem plástica pode suscitar nuances de dessemelhança e produzir sentidos que vão além dos efeitos resultantes pela relação analógica entre representação e realidade.
A busca por uma compreensão unitária dos direitos fundamentais
Authors: Barros Solange Aparecida Barbosa de Morais , Buzato Luiz Fernando Taques Fonseca
Published: June 2022

SCHAFER, Jairo. Classificação dos direitos fundamentais: do sistema geracional ao sistema unitário: uma proposta de compreensão. Porto Alegre: Livraria do Advogado, 2005.
Crenças e ensino de línguas: foco no professor, no aluno e na formação de professores
Authors: Jasnievski Célia Carrião
Published: June 2022

BARCELOS , Ana M. F e ABRAHÃO, Maria H. V. (Orgs.). Crenças e ensino de línguas: foco no professor, no aluno e na formação de professores. Campinas, SP: Pontes Editores, 2006.
Apresentação
Authors: Mezzomo Frank Antônio
Published: June 2022

Apresentação
Ramón Fogel: breves perspectivas histórico-sociais nas relações entre Brasil e Paraguai
Authors: Baller Leandro , Silva Giovani José da
Published: June 2022

Entrevista com Ramón Fogel
As representações do sertão na visão de Antônio Rolim de Moura
Authors: Canova Loiva
Published: June 2022

O sertão, a partir dos escritos de Antônio Rolim de Moura, ganha uma pluralidade de significados e aspectos diferenciados que compõem um conjunto de representações expressas em juízos, saberes, intenções e projeções. No fazer cotidiano da colonização, nas descobertas do novo espaço e dos personagens singulares que o habitavam, nas estratégias de sobrevivência diante dos perigos e hostilidades da natureza bruta, no gozo de suas paisagens, no aproveitamento dos seus recursos, o sertão se transforma. É com este sentido que se reflete as imagens construídas por Antônio Rolim de Moura do sertão matogrossense no setecentos.
Salve!, amanhã é outro dia!” o medo e a culpa do homem austríaco na segunda guerra mundial
Authors: Mendes Domenica Cristina
Published: June 2022

O presente artigo tem como objetivo apresentar um panorama geral da sociedade austríaca no ano de 1945. Com base em um romance literário de autoria de Johannes Mario Simmel, escritor austríaco que sobreviveu aos acontecimentos da Segunda Guerra Mundial, é possível analisar e identificar o medo e a culpa que os austríacos, independente de sua classe social, idade, ideologia e religião sentiam nesse período. Johannes apresenta uma história baseada em fatos reais, da qual participou, onde assume uma posição crítica e resistente ao movimento nazista e aos conflitos da guerra.
A construção social da identidade de gênero e as mulheres na prisão
Authors: Priori Claudia
Published: June 2022

Este artigo discute a questão da identidade cultural enquanto uma construção social e histórica, bem como a construção social da identidade de gênero, especialmente as múltiplas identidades femininas, uma vez que a identidade não é única e estável. Identidade e Gênero são dois conceitos muito dinâmicos que dependendo o contexto social, político e econômico em que ocorrem assumem significados e efeitos de sentido distintos. Além disso, o artigo trata também da identidade feminina nas prisões, em que o gênero é utilizado marcadamente para definir as relações sociais.
Território e identidade: apontamentos sobre a (i)migração ucraniana no centro do estado do Paraná (1895-1960)
Authors: Hahn Fábio André , Luz Leandro Domingos
Published: June 2022

Território e identidade: apontamentos sobre a (i)migração ucraniana no centro do estado do Paraná (1895-1960)
As contribuições teóricas do sociólogo Pierre Bourdieu: reflexões acerca da construção de um objeto de pesquisa
Authors: Cruz Ricardo Alexandre da
Published: June 2022

As contribuições teóricas do sociólogo Pierre Bourdieu: reflexões acerca da construção de um objeto de pesquisa
Impacts of Various Nutraceutical Milk Replacer Supplements on the Health and Performance of High-Risk Holstein Calves
Authors: Davis Emily M. , Liang Yu , Batchelder Tyler A. , Sellers Matthew D. , Ballou Michael A.
Published: June 2022

The objectives of this study were to determine the impacts of supplementing various nutraceuticals in milk replacers, including a blend of probiotics, β-glucan extract, mannanoligosaccharide extract, or a non-immunoglobulin rich extract, from colostrum on the performance and health of high-risk calves and to determine carry-over effects into the immediate post-weaned period. One hundred bull calves were acquired from a local calf ranch within 24 h of birth and randomly assigned to one of five dietary treatments added to milk replacer only: (1) Control (CON), no additive; (2) Immu-PRIME (ImmPr), 1.5 g/d ImmPr first 3 d only (Sterling Technology, Brookings, SD, USA); (3) Beta glucan (BG), 1 g/d BG extract (ImmuOligo, Irvine, CA, USA); (4) Mannanoligosaccharide + Bacillus subtilis (MOS+Bs), 3 g/d CEREVIDA EXCELL-M + 4 x 109 CFU/d Bacillus subtilis (MB Nutritional Sciences, Lubbock, TX, USA); and (5) PROVIDA CALF (PRO), proprietary blend of 2 x 109 CFU/d of a Lactobacillus casei and an Enterococcus faecium + 2 x 109 CFU/d Saccharomyces cerevisiae (MB Nutritional Sciences, Lubbock, TX, USA). Calves were weaned at d 56, comingled, and treatment carry-over effects evaluated through d 84. Starter intake was measured daily and BW weekly. Peripheral blood samples were collected and analyzed for hematology and serum was analyzed for haptoglobin concentrations. Polymorphonuclear leukocyte (PMNL) function was assessed through surface L-selectin expression, phagocytic, and oxidative burst activities against Escherichia coli. Data were analyzed using Proc Mixed in SAS (SAS 9.4, Cary, NC). The BG calves consumed the most starter from d 1 to 28 and pre-weaned average daily gain was greater for both the PRO and BG when compared to the CON group. There was a tendency for BG, MOS+Bs, and PRO to have reduced serum haptoglobin throughout the study. Total leukocyte, neutrophil, and lymphocyte counts were reduced among MOS+Bs calves, whereas BG calves tended to have the greatest neutrophil:lymphocyte ratio. Oxidative burst function for PMNL was reduced among BG and PRO treatments, suggesting a decreased need for neutrophil function. Supplementing BG, MOS+Bs, and PRO all influenced the performance and health of high-risk calves, but mechanistically appear to be different.
Patellar Tendon Shear Wave Velocity Is Higher and has Different Regional Patterns in Elite Competitive Alpine Skiers than in Healthy Controls
Authors: Götschi Tobias , Hanimann Jonas , Schulz Nicole , Huser Simon , Held Victoria , Frey Walter O. , Snedeker Jess G. , Spörri Jörg
Published: June 2022

Competitive alpine skiers are exposed to enormous forces acting on their bodies–particularly on the knee joint and hence the patellar tendon - during both the off-season preparation and in-season competition phases. However, factors influencing patellar tendon adaptation and regional pattern differences between alpine skiers and healthy controls are not yet fully understood, but are essential for deriving effective screening approaches and preventative countermeasures. Thirty elite competitive alpine skiers, all members of the Swiss Alpine Ski Team, and 38 healthy age-matched controls were recruited. A set of two-dimensional shear wave elastography measurements of the PT was acquired and projected into three-dimensional space yielding a volumetric representation of the shear wave velocity profile of the patellar tendon. Multivariate linear models served to quantify differences between the two cohorts and effects of other confounding variables with respect to regional shear wave velocity. A significant (p < 0.001) intergroup difference was found between skiers (mean ± SD = 10.4 ± 1.32 m/s) and controls (mean ± SD = 8.9 ± 1.59 m/s). A significant sex difference was found within skiers (p = 0.024), but no such difference was found in the control group (p = 0.842). Regional SWV pattern alterations between skiers and controls were found for the distal region when compared to the mid-portion (p = 0.023). Competitive alpine skiers exhibit higher SWV in all PT regions than healthy controls, potentially caused by long-term adaptations to heavy tendon loading. The presence of sex-specific differences in PT SWV in skiers but not in controls indicates that sex effects have load-dependent dimensions. Alterations in regional SWV patterns between skiers and controls suggest that patellar tendon adaptation is region specific. In addition to the implementation of 3D SWE, deeper insights into long-term tendon adaptation and normative values for the purpose of preventative screening are provided.
A Review on the Construction of Carbon-Based Metal Compound Composite Cathode Materials for Room Temperature Sodium-Sulfur Batteries
Authors: Wang Xueyu , Guo Daying , Yang Lin , Jin Minghuan , Chen Xi’an , Wang Shun
Published: June 2022

Room temperature sodium-sulfur batteries are one of the most attractive energy storage systems due to their low cost, environmental friendliness, and ultra-high energy density. However, due to the inherent slow redox kinetics and the shuttle of polysulfides, the road of room temperature sodium-sulfur batteries to practical application is still full of difficulties. As a sulfur cathode, which is directly related to battery performance, a lot of research efforts have been devoted to it and many strategies have been proposed to solve the shuttle effect problem of sulfur cathodes. This paper analyzes the existing problems and solutions of sodium-sulfur batteries, mainly discusses and summarizes the research progress of constructing carbon-based cathode materials for sodium-sulfur batteries, and expounds the current research popular from two main directions. That is to construct advanced cathode materials based on two mechanisms of adsorption and electrocatalysis. Finally, the research direction of advanced sodium-sulfur batteries is prospected.
Long-Term Outcomes for Chinese COPD Patients After PCI: A Propensity Score Matched, Double-Cohort Study
Authors: Zheng Yitian , Qi Yu , Seery Samuel , Wang Wenyao , Zhao Wei , Shen Tao , Zhou Lequn , Yang Jie , Li Chen , Wang Xuliang , Gao Jun , Meng Xiangbin , Dong Erdan , Tang Yi-Da
Published: June 2022

Objectives

The aim of this study was to analyze long-term outcomes of Chinese coronary artery disease (CAD) patients with (and without) chronic obstructive pulmonary disease (COPD) after percutaneous coronary intervention (PCI).

Background

Chronic obstructive pulmonary disease is a chronic condition which often develops in conjunction with CAD. PCI is a core therapy for CAD, although we still need to understand CAD-COPD outcomes and to identify factors that influence prognoses, across ethnicities.

Methods

This double-cohort study involved 12,343 Chinese CAD patients who received PCI. Baseline characteristics were collected in two independent, specialty centers. Propensity-score matching was performed to control confounding factors, using a nearest neighbor matching method within a 0.02 caliper and on a propensity score scale of 0.1 for each center. Comorbid CAD-COPD cases were compared to non-COPD patients in terms of major adverse cardiac events (MACEs).

Results

Patients with COPD were generally older than those without COPD (65.4 ± 9.2 vs. 58.2 ± 10.3, p < 0.001). There were no significant differences in the end points between COPD and non-COPD groups after PCI (All p > 0.05); however, the incidence of MACEs increased after 450 days. Further subgroup analysis suggests that COPD is approximately four times more prevalent among those aged over 75 years (HR, 3.818; 95%CI, 1.10–13.29; p = 0.027) and those aged below 55 years (HR = 4.254; 95% CI, 1.55–11.72; p = 0.003).

Conclusion

Having COPD does not appear to have a significant impact on CAD outcomes 2 years after PCI, and beyond. However, an increasing number of MACEs was observed after 450 days, which suggests that there may be a double-stage effect of COPD on PCI prognosis. There is a need for focused comorbidity management, specifically for those aged below 55 years and above 75 years.
Assessing the Hemodynamic Impact of Anterior Leaflet Laceration in Transcatheter Mitral Valve Replacement: An in silico Study
Authors: Kohli Keshav , Wei Zhenglun Alan , Sadri Vahid , Siefert Andrew W. , Blanke Philipp , Perdoncin Emily , Greenbaum Adam B. , Khan Jaffar M. , Lederman Robert J. , Babaliaros Vasilis C. , Yoganathan Ajit P. , Oshinski John N.
Published: June 2022

Background

A clinical study comparing the hemodynamic outcomes of transcatheter mitral valve replacement (TMVR) with vs. without Laceration of the Anterior Mitral leaflet to Prevent Outflow Obstruction (LAMPOON) has never been designed nor conducted.

Aims

To quantify the hemodynamic impact of LAMPOON in TMVR using patient-specific computational (in silico) models.

Materials

Eight subjects from the LAMPOON investigational device exemption trial were included who had acceptable computed tomography (CT) data for analysis. All subjects were anticipated to be at prohibitive risk of left ventricular outflow tract (LVOT) obstruction from TMVR, and underwent successful LAMPOON immediately followed by TMVR. Using post-procedure CT scans, two 3D anatomical models were created for each subject: (1) TMVR with LAMPOON (performed procedure), and (2) TMVR without LAMPOON (virtual control). A validated computational fluid dynamics (CFD) paradigm was then used to simulate the hemodynamic outcomes for each condition.

Results

LAMPOON exposed on average 2 ± 0.6 transcatheter valve cells (70 ± 20 mm2 total increase in outflow area) which provided an additional pathway for flow into the LVOT. As compared to TMVR without LAMPOON, TMVR with LAMPOON resulted in lower peak LVOT velocity, lower peak LVOT gradient, and higher peak LVOT effective orifice area by 0.4 ± 0.3 m/s (14 ± 7% improvement, p = 0.006), 7.6 ± 10.9 mmHg (31 ± 17% improvement, p = 0.01), and 0.2 ± 0.1 cm2 (17 ± 9% improvement, p = 0.002), respectively.

Conclusion

This was the first study to permit a quantitative, patient-specific comparison of LVOT hemodynamics following TMVR with and without LAMPOON. The LAMPOON procedure achieved a critical increment in outflow area which was effective for improving LVOT hemodynamics, particularly for subjects with a small neo-left ventricular outflow tract (neo-LVOT).
Experience and Discussion on Introducing the “Internet Plus” Classroom Into Teaching Physiological Experimentation
Authors: Guo Yan-hong , Chen Jian , Xin Min , Wang Yong , Tan Jie , Tian Jing , Liao Wei-yong , Zhang Xiao-ling , Su Min , Pang Yong-jun , Huang Wen-jun
Published: June 2022

The “Internet Plus” education model, enabled by the internet, is a type of “Distance learning” education models. To explore the application of “Internet Plus” education in teaching physiological experimentation at Guilin Medical University, undergraduate clinical medicine students from the 2011 to 2013 classes were used as the historical control group for teaching an experimentation class using the traditional teaching model: “teacher-teaching + student-operation.” Undergraduate clinical medicine students from the 2014 to 2018 classes were used as the teaching reform group and were taught using an “Internet Plus” educational model: “microlectures-previewing + test + feedback + student-operation.” The experimentation operating examination results for the two groups of students were analyzed, questionnaire surveys were administered to students in the experimental group and the relevant teachers, and the survey results were counted. The experimentation operating examination results were significantly higher for the students in the teaching reform group (89.3 points) than for the control group students (84.4 points). The excellent rate was higher (69.8% for the reform group and 54.5% for the control group), whereas the failure rate was lower (1.9% for the teaching reform group and 4.3% for the control group). Additionally,90.1% of the students identified with this reform method, and more than 90% believed that the method improved their interest and confidence in surgical operations during pharmacological and pathophysiological experimentation classes in subsequent courses. All teachers in the physiological experimentation course identified with this teaching method, and 81.8% of the teachers of relevant disciplines in a later stage believed that this learning method was more conducive than traditional teaching to cultivating student interest in learning, proactivity, and other factors. Application of an “Internet Plus” educational model for physiological experimentation teaching can help cultivate hands-on operating abilities and improve the learning interest of students.
Research on China's Environmental Governance Mode: Quality Driven or Quantity Driven?
Authors: Ma Yubin , Chen Haotian , Zhao Yating , Li Zhengda
Published: June 2022

High-quality environmental governance (EG) is closely related to its governance mode. Nevertheless, few studies have examined the EG modes from the dual perspectives of quality and quantity. This article utilizes panel data of 30 Chinese provinces from 2003 to 2020 to research the influence of environmental governance efficiency (EGE) and investment (EGI) on EG through a fixed-effect mode. The outcomes show that China’s EG is driven mainly by quantitative EGI. EGE and EGI show significant geographic regions, economic development levels, resource endowments, and stage heterogeneity to EG. In light of these conclusions, this article argues that the future needs to reasonably allocate EGI based on consideration of the heterogeneity of geographical regions, economic development levels, and resource endowments to optimize EGI structure and increase EGE in each province to achieve high-quality EG.
Oral Phenotype of Singleton–Merten Syndrome: A Systematic Review Illustrated With a Case Report
Authors: Riou Margot Charlotte , de La Dure-Molla Muriel , Kerner Stéphane , Rondeau Sophie , Legendre Adrien , Cormier-Daire Valerie , Fournier Benjamin P. J.
Published: June 2022

Background: Singleton–Merten syndrome type 1 (SGMRT1) is a rare autosomal dominant disorder caused by IFIH1 variations with blood vessel calcifications, teeth anomalies, and bone defects.

Aim: We aimed to summarize the oral findings in SGMRT1 through a systematic review of the literature and to describe the phenotype of a 10-year-old patient with SGMRT1 diagnosis.

Results: A total of 20 patients were described in the literature, in nine articles. Eight IFIH1 mutations were described in 11 families. Delayed eruption, short roots, and premature loss of permanent teeth were the most described features (100%). Impacted teeth (89%) and carious lesions (67%) were also described. Our patient, a 10-year-old male with Singleton–Merten syndrome, presented numerous carious lesions, severe teeth malposition, especially in the anterior arch, and an oral hygiene deficiency with a 100% plaque index. The panoramic X-ray did not show any dental agenesis but revealed very short roots and a decrease in the jaw alveolar bone height. The whole-genome sequencing analysis revealed a heterozygous de novo variant in IFIH1 (NM_022168.4) c.2465G > A (p.Arg822Gln).

Conclusion: Confused descriptions of oral features occurred in the literature between congenital findings and “acquired” pathology, especially carious lesions. The dental phenotype of these patients encompasses eruption anomalies (delayed eruption and impacted teeth) and lack of root edification, leading to premature loss of permanent teeth, and it may contribute to the diagnosis. An early diagnosis is essential to prevent teeth loss and to improve the quality of life of these patients.

Systematic Review Registration: [https://www.crd.york.ac.uk/prospero/], identifier [CRD42022300025].
A Prospective Review on Selectable Marker-Free Genome Engineered Rice: Past, Present and Future Scientific Realm
Authors: Singh Rajveer , Kaur Navneet , Praba Umesh Preethi , Kaur Gurwinder , Tanin Mohammad Jafar , Kumar Pankaj , Neelam Kumari , Sandhu Jagdeep Singh , Vikal Yogesh
Published: June 2022

As a staple food crop, rice has gained mainstream attention in genome engineering for its genetic improvement. Genome engineering technologies such as transgenic and genome editing have enabled the significant improvement of target traits in relation to various biotic and abiotic aspects as well as nutrition, for which genetic diversity is lacking. In comparison to conventional breeding, genome engineering techniques are more precise and less time-consuming. However, one of the major issues with biotech rice commercialization is the utilization of selectable marker genes (SMGs) in the vector construct, which when incorporated into the genome are considered to pose risks to human health, the environment, and biodiversity, and thus become a matter of regulation. Various conventional strategies (co-transformation, transposon, recombinase systems, and MAT-vector) have been used in rice to avoid or remove the SMG from the developed events. However, the major limitations of these methods are; time-consuming, leftover cryptic sequences in the genome, and there is variable frequency. In contrast to these methods, CRISPR/Cas9-based marker excision, marker-free targeted gene insertion, programmed self-elimination, and RNP-based delivery enable us to generate marker-free engineered rice plants precisely and in less time. Although the CRISPR/Cas9-based SMG-free approaches are in their early stages, further research and their utilization in rice could help to break the regulatory barrier in its commercialization. In the current review, we have discussed the limitations of traditional methods followed by advanced techniques. We have also proposed a hypothesis, “DNA-free marker-less transformation” to overcome the regulatory barriers posed by SMGs.
Construction and Validation of a Novel Prognostic Signature of Idiopathic Pulmonary Fibrosis by Identifying Subtypes Based on Genes Related to 7-Methylguanosine Modification
Authors: Huang Tao , He Wei-Ying
Published: June 2022

Background: Idiopathic pulmonary fibrosis (IPF) is the interstitial lung disease with the highest incidence and mortality. The lack of specific markers results in limited treatment methods for IPF patients. Numerous prognostic signatures represented effective indexes in predicting the survival of patients in various diseases; however, little is investigated on their application in IPF.

Methods: This study attempted to explore the clinical markers suitable for IPF by constructing a prognostic signature from the perspective of 7-methylguanosine (m7G). An m7G-related prognostic signature (m7GPS) was established based on the discovery cohort with the LASSO algorithm and was verified by internal and external validation cohorts. The area under the curve (AUC) values were utilized to assess the accuracy of m7GPS in predicting the prognosis of IPF patients and the ability of m7GPS in screening IPF patients. Kaplan-Meier curves and Cox regression analyses were used to identify the relationship of m7GPS with the prognosis of IPF individuals. Enrichment analyses, CIBERSORT algorithm, and weighted gene co-expression network analysis were applied to explore the underlying mechanisms and correlation of m7GPS in IPF.

Results: The two m7G regulatory genes can divide IPF into subtypes 1 and 2, and subtype 2 demonstrated a poor prognosis for IPF patients (p < 0.05). For the first time in this field, the m7GPS was constructed. m7GPS made it feasible to predict the 1–5 years survival status of IPF patients (AUC = 0.730–0.971), and it was an independent prognostic risk factor for IPF patients (hazard ratio > 1, p < 0.05). The conspicuous ability of m7GPS to screen IPF patients from the healthy was also revealed by an AUC value of 0.960. The roles of m7GPS in IPF may link to inflammation, immune response, and immune cell levels. Seven genes (CYR61, etc.) were identified as hub genes of m7GPS in IPF. Three drugs (ZM447439-1050, AZD1332-1463, and Ribociclib-1632) were considered sensitive to patients with high m7GPS risk scores.

Conclusion: This study developed a novel m7GPS, which is a reliable indicator for predicting the survival status of IPF patients and is identified as an effective marker for prognosis and screening of IPF patients.
Clinical Values of the Identified Hub Genes in Systemic Lupus Erythematosus
Authors: Xiao Lu , Zhan Feng , Lin Shudian
Published: June 2022

Objective

This study was conducted to identify the biomarkers and mechanisms associated with systemic lupus erythematosus(SLE) at a transcriptome level.

Methods

Microarray datasets were downloaded, and differentially expressed genes (DEGs) were identified. Enrichment and protein–protein interaction networks were analyzed, and hub genes were discovered. The levels of top 10 hub genes were validated by another dataset. The diagnostic accuracy of the hub genes was evaluated with the area under the curve of the receiver operating characteristic curve (ROC-AUC). The odds ratios (OR) and 95% confidence intervals (CI) of the relationship between clinical manifestations and hub genes were estimated with multivariable logistic regression. The relationships between the expression levels of the 10 identified hub genes and SLEDAI scores were subjected to linear correlation analysis. Changes in the expression levels of the hub genes during patient follow-up were examined through one-way repeated measures ANOVA.

Results

A total of 136 DEGs were identified. Enrichment analysis indicated that DEGs were primarily enriched in type I interferon-associated pathways. The identified hub genes were verified by the GSE65391 dataset. The 10 hub genes had good diagnostic performances. Seven (except IFI6, OAS1 and IFIT3) of the 10 hub genes were positively associated with SLEDAI. The combination models of IFIT3, ISG15, MX2, and IFIH1 were effective in diagnosing mucosal ulcers among patients with SLE. The expression levels of IRF7, IFI35, IFIT3, and ISG15 decreased compared with the baseline expression (not significantly).

Conclusions

In this work, the clinical values of the identified hub genes in SLE were demonstrated.
Factors Associated With Deep Sedation Practice in Mechanically Ventilated Patients: A Post hoc Analysis of a Cross–Sectional Survey Combined With a Questionnaire for Physicians on Sedation Practices
Authors: Ma Penglin , Wang Tao , Gong Yichun , Liu Jingtao , Shi Wei , Zeng Lin
Published: June 2022

Purpose

The study aimed to explore factors associated with deep sedation practice in intensive care units (ICUs).

Materials and Methods

A post hoc analysis was conducted for a cross–sectional survey on sedation practices in mechanically ventilated (MV) patients, combined with a questionnaire for physicians regarding their preferences for light sedation (P–pls Score) in 92 Chinese ICUs.

Results

There were 457 and 127 eligible MV patients in the light and deep sedation groups respectively. A multivariable logistic regression analysis demonstrated that the control mode of mechanical ventilation, plasma lactate level, and the Sequential Organ Failure Assessment (SOFA) score were independent risk factors for deep sedation practice (p <0.01). Notably, the adjusted odds ratio (95% CI) of the average P–pls score in the ICU ≤ 2 for deep sedation practice was 1.861 (1.163, 2.978, p = 0.01). In addition, the areas under curves of receiver operating characteristics (AUC–ROC) of the model to predict the probability of deep sedation practice were 0.753 (0.699, 0.806) and 0.772 (0.64, 0.905) in the training set and the validation set, respectively. The 28–day mortality was increased in patients with exposure to deep sedation practice but not significantly.

Conclusion

Both factors related to stressful stimuli and the ICU physicians' perception of patient tolerability in mechanical ventilation were likely associated with deep sedation practice in MV patients.
Optical Coherence Tomography Biomarkers in Predicting Treatment Outcomes of Diabetic Macular Edema After Dexamethasone Implants
Authors: Huang Yu-Te , Chang Yen-Chieh , Meng Ping-Ping , Lin Chun-Ju , Lai Chun-Ting , Hsia Ning-Yi , Chen Huan-Sheng , Tien Peng-Tai , Bair Henry , Lin Jane-Ming , Chen Wen-Lu , Tsai Yi-Yu
Published: June 2022

Purpose

To identify optical coherence tomography (OCT) biomarkers that may predict functional and anatomical outcomes in diabetic macular edema (DME) patients treated with intravitreal dexamethasone (DEX) implant.

Materials and Methods

Sixty-four eyes from 50 patients with DME were enrolled. Best-corrected visual acuity (BCVA) and OCT biomarkers including central retinal thickness (CRT), subretinal fluid (SRF), intraretinal cysts (IRC), ellipsoid zone disruption (EZD), disorganization of retinal inner layers (DRIL), hard exudate (HE), hyperreflective foci (HRF), epiretinal membrane (ERM), and vitreomacular interface (VMI) changes were evaluated at baseline and at 3, 6, and 12 months after treatment. Multiple logistic analysis was performed to evaluate each OCT biomarker as a predictive factor for functional and anatomical improvement at the end of treatment.

Results

The presence of SRF at baseline was associated with a favorable outcome, with CRT improving by more than 100 μm after treatment from multivariate logistic regression analysis [odds ratio 6.16 (1.75–21.6)]. In addition, baseline SRF predicted a greater CRT improvement from multiple regression analysis (model R-square 0.11, p = 0.006). The reduction of DRIL, SRF, LONLC, IRC, and EZD were correlated with better CRT improvement (more than 100 μm) (P < 0.05). SRF and EZD recovery can also predict better visual prognosis (P < 0.05).

Conclusion

OCT biomarkers can be used to predict who may benefit the most after DEX treatment. We suggest that the DEX implant should be considered as a first line treatment in DME patients with SRF.
Road Toward a New Model of Care for Idiopathic Pulmonary Fibrosis in the Lazio Region
Authors: Di Bidino Rossella , Rogliani Paola , Sebastiani Alfredo , Ricci Alberto , Varone Francesco , Sgalla Giacomo , Iovene Bruno , Bruni Teresa , Flore Maria Chiara , D'Ascanio Michela , Cavalli Francesco , Savi Daniela , Di Michele Loreta , Cicchetti Americo , Richeldi Luca
Published: June 2022

A timely, confirmed diagnosis of Idiopathic Pulmonary Fibrosis (IPF) has a significant impact on the evolution of the disease. The current model of care in the Lazio region (in Italy) was assessed on the basis of real-world data provided by the four reference centers responsible for diagnosing and treating IPF. The 5-year, population-based, retrospective longitudinal study provided the data that is at the basis of the current proposal for a new clinical and therapeutic pathway (DTCP) and has been shared with regional decision makers. A DTCP must be defined and based on four pillars: GPs, pulmonologists, IPF centers, and telemedicine. Each must play a role within a sort of hub-and-spoke model. IPF centers remain the hubs, while spokes are identified in trained GPs and pulmonologists.
The Clinical and Genetic Features in Chinese Children With Steroid-Resistant or Early-Onset Nephrotic Syndrome: A Multicenter Cohort Study
Authors: Zhu Xiujuan , Zhang Yanqin , Yu Zihua , Yu Li , Huang Wenyan , Sun Shuzhen , Li Yingjie , Wang Mo , Li Yongzhen , Sun Liangzhong , Yang Qing , Deng Fang , Shao Xiaoshan , Liu Ling , Liu Cuihua , Qin Yuanhan , Feng Shipin , Zhu Hongtao , Yang Fang , Zheng Weimin , Zheng Wanqi , Zhong Rirong , Hou Ling , Mao Jianhua , Wang Fang , Ding Jie
Published: June 2022

Steroid-resistant nephrotic syndrome (SRNS) is one of the major causes of end-stage kidney disease (ESKD) in children and young adults. For approximately 30% of children with SRNS results from a genetic cause. In this study, genotype-phenotype correlations in a cohort of 283 pediatric patients with SRNS or early-onset NS (nephrotic syndrome presenting within the first year of life) from 23 major pediatric nephrology centers in China were analyzed. All patients were performed with next-generation sequencing and Sanger sequencing. The overall mutation detection rate was 37.5% (106 of 283 patients). WT1 was the most frequently detected mutation, followed by NPHS1, NPHS2, and ADCK4, and these four major causative genes (WT1, NPHS1, NPHS2, and ADCK4) account for 73.6% of patients with monogenic SRNS. Thirteen of 106 individuals (12.3%) carried mutations in ADCK4 that function within the coenzyme Q10 biosynthesis pathway. In the higher frequently ADCK4-related SRNS, two mutations, c.737G>A (p.S246N) and c.748G>C (p.D250H), were the most prevalent. Our study provides not only definitive diagnosis but also facilitate available targeted treatment for SRNS, and prediction of prognosis and renal outcome. Our indications for genetic testing are patients with FSGS, initial SRNS, cases of positive family history or those with extra-renal manifestations.
Case Report: The Experience of Managing a Moderate ARDS Caused by SARS-CoV-2 Omicron BA.2 Variant in Chongqing, China: Can We Do Better?
Authors: Peng Junnan , Li Qiaoli , Dong Jing , Yuan Guodan , Wang Daoxin
Published: June 2022

Background

The severe coronavirus disease 2019 (COVID-19) pandemic is still raging worldwide, and the Omicron BA.2 variant has become the new circulating epidemic strain. However, our understanding of the Omicron BA.2 variant is still scarce. This report aims to present a case of a moderate acute respiratory distress syndrome (ARDS) caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Omicron BA.2 variant and to discuss some management strategies that may benefit this type of case.

Case Presentation

A 78-year-old man, who had four negative nucleic acid tests and a fifth positive, was admitted to our hospital. This patient was generally good upon admission and tested negative for anti-SARS-CoV-2 antibodies even after receiving two doses of the COVID-19 vaccine. On the 7th day of hospitalization, he developed a moderate ARDS. Improved inflammatory index and decreased oxygen index were primarily found in this patient, and a series of treatments, including anti-inflammation and oxygen therapies, were used. Then this patient’s condition improved soon and reached two negative results of nucleic acid tests on the 18th day of hospitalization.

Conclusion

At-home COVID-19 rapid antigen test could be complementary to existing detection methods, and the third booster dose of COVID-19 vaccine may be advocated in the face of the omicron BA.2 variant. Anti-inflammatory and oxygen therapies are still essential treatments for ARDS patients infected with SARS-CoV-2 Omicron BA.2 variant.
A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 Months
Authors: Mandhan Prerna , Sharma Mansi , Pandey Sushmita , Chandel Neha , Chourasia Nidhi , Moun Amit , Sharma Divyani , Sukar Rubee , Singh Niyati , Mathur Shubhangi , Kotnala Aarti , Negi Neetu , Gupta Ashish , Kumar Anuj , Suresh Kumar R. , Kumar Pramod , Singh Shalini
Published: June 2022

An effective and rapid diagnosis has great importance in tackling the ongoing COVID-19 pandemic through isolation of the infected individuals to curb the transmission and initiation of specialized treatment for the disease. It has been proven that enhanced testing capacities contribute to efficiently curbing SARS-CoV-2 transmission during the initial phases of the outbreaks. RT-qPCR is considered a gold standard for the diagnosis of COVID-19. However, in resource-limited countries expenses for molecular diagnosis limits the diagnostic capacities. Here, we present interventions of two pooling strategies as 5 sample pooling (P-5) and 10 sample pooling (P-10) in a high-throughput COVID-19 diagnostic laboratory to enhance throughput and save resources and time over a period of 6 months. The diagnostic capacity was scaled-up 2.15-folds in P-5 and 1.8-fold in P-10, reagents (toward RNA extraction and RT-qPCR) were preserved at 75.24% in P-5 and 86.21% in P-10, and time saved was 6,290.93 h in P-5 and 3147.3 h in P-10.
Palmitic Amide Triggers Virus Life Cycle via Enhancing Host Energy Metabolism
Authors: Zhang Xinyi , Zhuang Jianjian , Huang Liquan , Zhang Xiaobo
Published: June 2022

Viruses contribute to the mortality of organisms, consequentially altering biological species composition of an ecosystem and having a threat on human health. As the most famous model for the initiation of virus infection, the Hershey-Chase experiment has revealed that on infection, the bacteriophage genomic DNA is injected into its host bacterium, while the viral capsid is left on the outer membrane of host cell. However, little is known about the injection of any other materials into the cytoplasm of host cells along with genomic DNA to trigger the virus life cycle. In this study, the results showed that palmitic amide packaged in the virions of GVE2, a bacteriophage infecting deep-sea hydrothermal vent thermophile Geobacillus sp. E263, promoted virus infection. Palmitic amide was interacted with acetate kinase to increase its enzymatic activity, thus enhancing the acetate-mediated energy metabolism. Furthermore, palmitic amide promoted tricarboxylic acid cycle (TCA cycle) to support virus infection. These data indicated that palmitic amide, packaged in the virions, might serve as a second messenger at the initiation step of virus infection by enhancing the host energy metabolism. Therefore our study revealed a novel mechanism for the initiation of the virus life cycle.