bluuebunny/crossref_metadata_2025 huggingface dataset:
Impacts of observation frequency on proximity contact data and modeled transmission dynamics
Authors: Qian Weicheng , Stanley Kevin Gordon , Osgood Nathaniel David
Published: February 2023

Transmission of many communicable diseases depends on proximity contacts among humans. Modeling the dynamics of proximity contacts can help determine whether an outbreak is likely to trigger an epidemic. While the advent of commodity mobile devices has eased the collection of proximity contact data, battery capacity and associated costs impose tradeoffs between the observation frequency and scanning duration used for contact detection. The choice of observation frequency should depend on the characteristics of a particular pathogen and accompanying disease. We downsampled data from five contact network studies, each measuring participant-participant contact every 5 minutes for durations of four or more weeks. These studies included a total of 284 participants and exhibited different community structures. We found that for epidemiological models employing high-resolution proximity data, both the observation method and observation frequency configured to collect proximity data impact the simulation results. This impact is subject to the population’s characteristics as well as pathogen infectiousness. By comparing the performance of two observation methods, we found that in most cases, half-hourly Bluetooth discovery for one minute can collect proximity data that allows agent-based transmission models to produce a reasonable estimation of the attack rate, but more frequent Bluetooth discovery is preferred to model individual infection risks or for highly transmissible pathogens. Our findings inform the empirical basis for guidelines to inform data collection that is both efficient and effective.
The association of different presentations of maternal depression with children’s socio-emotional development: A systematic review
Authors: Morales María Francisca , Girard Lisa-Christine , Raouna Aigli , MacBeth Angus
Published: February 2023

Maternal depression from the perinatal period onwards is a global health priority associated with an increased likelihood of suboptimal socio-developmental outcomes in offspring. An important aspect of this association is the extent to which sustained maternal depression impacts on these outcomes. The current review synthesised the evidence on maternal depression from the perinatal period onwards and offspring internalising, externalising, and social competence outcomes. We also identified sources of methodological bias. A systematic review following PRISMA guidelines was conducted. Longitudinal studies targeting biological mothers with depressive symptomology, detailing onset, using repeated validated measures, and assessing children’s outcomes between three and 12 years were included. Twenty-four studies met inclusion criteria. Findings supported the validity of different presentations of maternal depression, including consistent identification of a group of chronically depressed mothers across countries. Mothers within this group reported poorer internalising, externalising, and social competence outcomes for their offspring, with the highest levels of child problems associated with greater maternal chronicity and symptom severity. Results differed by measurement type with mothers rating poorer outcomes in comparison to teachers reports. For timing of depression, evidence was inconsistent for independent effects of antenatal or postnatal depression on child outcomes. There was substantial variability in study quality assessment. Assessing different presentations of maternal depression is essential for capturing the longitudinal associations between maternal depression and offspring outcomes to inform targets of early intervention. Chronicity, severity, and concurrent maternal depression have important implications for children’s development and should be targeted in future programme planning. Further research in low- and middle-income countries is warranted.
Referral to the NHS Diabetes Prevention Programme and conversion from nondiabetic hyperglycaemia to type 2 diabetes mellitus in England: A matched cohort analysis
Authors: Ravindrarajah Rathi , Sutton Matt , Reeves David , Cotterill Sarah , Mcmanus Emma , Meacock Rachel , Whittaker William , Soiland-Reyes Claudia , Heller Simon , Bower Peter , Kontopantelis Evangelos
Published: February 2023

Background

The NHS Diabetes Prevention Programme (NDPP) is a behaviour change programme for adults who are at risk of developing type 2 diabetes mellitus (T2DM): people with raised blood glucose levels, but not in the diabetic range, diagnosed with nondiabetic hyperglycaemia (NDH). We examined the association between referral to the programme and reducing conversion of NDH to T2DM.

Methods and findings

Cohort study of patients attending primary care in England using clinical Practice Research Datalink data from 1 April 2016 (NDPP introduction) to 31 March 2020 was used. To minimise confounding, we matched patients referred to the programme in referring practices to patients in nonreferring practices. Patients were matched based on age (≥3 years), sex, and ≥365 days of NDH diagnosis. Random-effects parametric survival models evaluated the intervention, controlling for numerous covariates. Our primary analysis was selected a priori: complete case analysis, 1-to-1 practice matching, up to 5 controls sampled with replacement. Various sensitivity analyses were conducted, including multiple imputation approaches. Analysis was adjusted for age (at index date), sex, time from NDH diagnosis to index date, BMI, HbA1c, total serum cholesterol, systolic blood pressure, diastolic blood pressure, prescription of metformin, smoking status, socioeconomic status, a diagnosis of depression, and comorbidities.

A total of 18,470 patients referred to NDPP were matched to 51,331 patients not referred to NDPP in the main analysis. Mean follow-up from referral was 482.0 (SD = 317.3) and 472.4 (SD = 309.1) days, for referred to NDPP and not referred to NDPP, respectively. Baseline characteristics in the 2 groups were similar, except referred to NDPP were more likely to have higher BMI and be ever-smokers. The adjusted HR for referred to NDPP, compared to not referred to NDPP, was 0.80 (95% CI: 0.73 to 0.87) (p < 0.001). The probability of not converting to T2DM at 36 months since referral was 87.3% (95% CI: 86.5% to 88.2%) for referred to NDPP and 84.6% (95% CI: 83.9% to 85.4%) for not referred to NDPP. Associations were broadly consistent in the sensitivity analyses, but often smaller in magnitude. As this is an observational study, we cannot conclusively address causality. Other limitations include the inclusion of controls from the other 3 UK countries, data not allowing the evaluation of the association between attendance (rather than referral) and conversion.

Conclusions

The NDPP was associated with reduced conversion rates from NDH to T2DM. Although we observed smaller associations with risk reduction, compared to what has been observed in RCTs, this is unsurprising since we examined the impact of referral, rather than attendance or completion of the intervention.
MiR-942-5p inhibits tumor migration and invasion through targeting CST1 in esophageal squamous cell carcinoma
Authors: Zhang Liangming , Yu Sunxing , Yin Xiaoqing , Tu Mingshu , Cai Liqing , Zhang Yi , Yu Lili , Zhang Songgao , Pan Xiaojie , Huang Yi
Published: February 2023

Introduction

Cysteine Protease Inhibitor 1 (CST1), a cystatin superfamily protein with the effect on the inhibition of cysteine protease activity, is reported to be involved in the development of many malignancies. MiR-942-5p has been demonstrated its regulatory effects on some malignancies. However, the roles of CST1 and miR-942-5p on esophageal squamous cell carcinoma (ESCC) are still unknown up to now.

Methods

The expression of CST1 in ESCC tissues was analyzed by TCGA database, immunohistochemistry, and RT-qPCR, respectively. Matrigel-uncoated or-coated transwell assay was used to determine the effect of CST1 on migration and invasion of ESCC cells. Regulatory effect of miR-942-5p on CST1 was detected by dual luciferase assay.

Results

CST1 was ectopically highly expressed in ESCC tissues, and had the effect on promoting the migration and invasion of ESCC cells by upregulating phosphorylated levels of key effectors including MEK1/2, ERK1/2, and CREB in MEK/ERK/CREB pathway. Dual-luciferase assay results showed that miR-942-5p had a regulatory effect on targeting CST1.

Conclusions

CST1 plays a carcinogenic role on ESCC, and miR-942-5p can regulate the migration and invasion of ESCC cells by targeting CST1 to downregulate MEK/ERK/CREB signaling pathway, suggesting that miR-942-5p/CST1 axis might be a promising target for diagnosis and treatment of ESCC.
Patients’ preferences in dental care: A discrete-choice experiment and an analysis of willingness-to-pay
Authors: Felgner Susanne , Henschke Cornelia
Published: February 2023

Introduction

Dental diseases are a major problem worldwide. Costs are a burden on healthcare systems and patients. Missed treatments can have health and financial consequences. Compared to other health services, dental treatments are only covered in parts by statutory health insurance (SHI). Using the example of dental crowns for a cost-intensive treatment, our study aims to investigate whether (1) certain treatment attributes determine patients’ treatment choice, and (2) out-of-pocket payments represent a barrier to access dental care.

Methods

We conducted a discrete-choice-experiment by mailing questionnaires to 10,752 people in Germany. In presented scenarios the participants could choose between treatment options (A, B, or none) composed of treatment attribute levels (e.g., color of teeth) for posterior (PT) and anterior teeth (AT). Considering interaction effects, we used a D-efficient fractional factorial design. Choice analysis was performed using different models. Furthermore, we analyzed willingness-to-pay (WTP), preference of choosing no and SHI standard care treatment, and influence of socioeconomic characteristics on individual WTP.

Results

Out of n = 762 returned questionnaires (response rate of r = 7.1), n = 380 were included in the analysis. Most of the participants are in age group "50 to 59 years" (n = 103, 27.1%) and female (n = 249, 65.5%). The participants’ benefit allocations varied across treatment attributes. Aesthetics and durability of dental crowns play most important roles in decision-making. WTP regarding natural color teeth is higher than standard SHI out-of-pocket payment. Estimations for AT dominate. For both tooth areas, "no treatment" was a frequent choice (PT: 25.7%, AT: 37.2%). Especially for AT, treatment beyond SHI standard care was often chosen (49.8%, PT: 31.3%). Age, gender, and incentive measures (bonus booklet) influenced WTP per participant.

Conclusion

This study provides important insights into patient preferences for dental crown treatment in Germany. For our participants, aesthetic for AT and PT as well as out-of-pocket payments for PT play an important role in decision-making. Overall, they are willing to pay more than the current out-of-pockt payments for what they consider to be better crown treatments. Findings may be valuable for policy makers in developing measures that better match patient preferences.
Is massage a legitimate part of nursing care? A qualitative study
Authors: Da Rocha Rodrigues Gora , Anex Adrien , Boegli Monique , Bollondi Pauly Catherine , Curtin François , Luthy Christophe , Desmeules Jules , Cedraschi Christine
Published: February 2023

Introduction

The use of massage therapy has received increased attention in the treatment of chronic pain. However, barriers can hinder its use in nursing care. This study uses a qualitative methodology to explore professionals’ experiences regarding touch massage (TM) and identify barriers and facilitators for the implementation of this intervention.

Materials and methods

This study is part of a larger research program aimed at investigating the impact of TM on the experiences of patients with chronic pain hospitalized in two units of an internal medicine rehabilitation ward. Health care professionals (HCPs) were trained either to provide TM or to use of a massage-machine device according to their units. At the end of the trial, two focus groups were conducted with HCPs from each unit who took part in the training and agreed to discuss their experience: 10 caregivers from the TM group and 6 from the machine group. The focus group discussions were tape-recorded, transcribed and analyzed using thematic content analysis.

Results

Five themes emerged from thematic content analysis: perceived impact on patients, HCPs’ affective and cognitive experiences, patient-professionals relationships, organizational tensions, and conceptual tensions. Overall, the HCPs reported better general outcomes with TM than with the machine. They described positive effects on patients, HCPs, and their relationships. Regarding interventions’ implementation, the HCPs reported organizational barriers such as patients’ case complexity, work overload, and lack of time. Conceptual barriers such as ambivalence around the legitimacy of TM in nursing care were reported. TM was often described as a pleasure care that was considered a complementary approach and was overlooked despite its perceived benefits.

Conclusion

Despite the perceived benefits of TM reported by the HCPs, ambivalence arose around the legitimacy of this intervention. This result emphasizes the importance of changing HCPs’ attitudes regarding a given intervention to facilitate its implementation.
Involvement and possible role of transglutaminases 1 and 2 in mediating fibrotic signalling, collagen cross-linking and cell proliferation in neonatal rat ventricular fibroblasts
Authors: Al-U’datt Doa’a G. F. , Tranchant Carole C. , Al-Husein Belal , Hiram Roddy , Al-Dwairi Ahmed , AlQudah Mohammad , Al-shboul Othman , Jaradat Saied , Alqbelat Jenan , Almajwal Ali
Published: February 2023

Transglutaminase (TG) isoforms control diverse normal and pathophysiologic processes through their capacity to cross-link extracellular matrix (ECM) proteins. Their functional and signalling roles in cardiac fibrosis remain poorly understood, despite some evidence of TG2 involvement in abnormal ECM remodelling in heart diseases. In this study, we investigated the role of TG1 and TG2 in mediating fibrotic signalling, collagen cross-linking, and cell proliferation in healthy fibroblasts by siRNA-mediated knockdown. siRNA for TG1, TG2 or negative control was transfected into cultured neonatal rat ventricular fibroblasts and cardiomyocytes. mRNA expression of TGs and profibrotic, proliferation and apoptotic markers was assessed by qPCR. Cell proliferation and soluble and insoluble collagen were determined by ELISA and LC-MS/MS, respectively. TG1 and TG2 were both expressed in neonatal rat cardiomyocytes and fibroblasts before transfection. Other TGs were not detected before and after transfection. TG2 was predominantly expressed and more effectively silenced than TG1. Knocking down TG1 or TG2 significantly modified profibrotic markers mRNA expression in fibroblasts, decreasing connective tissue growth factor (CTGF) and increasing transforming growth factor-β1 compared to the negative siRNA control. Reduced expression of collagen 3A1 was found upon TG1 knockdown, while TG2 knockdown raised α-smooth muscle actin expression. TG2 knockdown further increased fibroblast proliferation and the expression of proliferation marker cyclin D1. Lower insoluble collagen content and collagen cross-linking were evidenced upon silencing TG1 or TG2. Transcript levels of collagen 1A1, fibronectin 1, matrix metalloproteinase-2, cyclin E2, and BCL-2-associated X protein/B-cell lymphoma 2 ratio were strongly correlated with TG1 mRNA expression, whereas TG2 expression correlated strongly with CTGF mRNA abundance. These findings support a functional and signalling role for TG1 and TG2 from fibroblasts in regulating key processes underlying myocardial ECM homeostasis and dysregulation, suggesting that these isoforms could be potential and promising targets for the development of cardiac fibrosis therapies.
Reverberation effect of communication in a public goods game
Authors: Bershadskyy Dmitri
Published: February 2023

Using a public goods laboratory experiment, this paper analyzes the extent to which face-to-face communication keeps its efficiency gains even after its removal. This is important as communication in real world is costly (e.g. time). If the effect of communication is long-lasting, the number of communication periods could be minimized. This paper provides evidence that there is a lasting positive effect on contributions even after communication was removed. Yet, after the removal, the contributions are lower and abate over time to the previous magnitude. This is referred to as the reverberation effect of communication. As we do not observe an effect of endogenizing communication, the strongest driver of the size of the contributions is the existence of communication or its reverberation. Eventually, the experiment provides evidence for a strong end-game effect after communication was removed, insinuating communication does not protect from the end-game behavior. In total, the results of the paper imply, that the effects of communication are not permanent but communication should be repeated. Simultaneously, results indicate no need for permanent communication. Since communication is conducted using video-conference tools, we present results from a machine learning based analysis of facial expressions to predict contribution behavior on group level.
Ankle exoskeleton torque controllers based on soleus muscle models
Authors: Pridham Paul S. , Stirling Leia
Published: February 2023

Powered exoskeletons are typically task-specific, but to facilitate their wider adoption they should support a variety of tasks, which requires generalizeable controller designs. In this paper, we present two potential controllers for ankle exoskeletons based on soleus fascicles and Achilles tendon models. The methods use an estimate of the adenosine triphosphate hydrolysis rate of the soleus based on fascicle velocity. Models were evaluated using muscle dynamics from the literature, which were measured with ultrasound. We compare the simulated behavior of these methods against each other and to human-in-the-loop optimized torque profiles. Both methods generated distinct profiles for walking and running with speed variations. One of the approaches was more appropriate for walking, while the other approach estimated profiles similar to the literature for both walking and running. Human-in-the-loop methods require long optimizations to set parameters per individual for each specific task, the proposed methods can produce similar profiles, work across walking and running, and be implemented with body-worn sensors without requiring torque profile parameterization and optimization for every task. Future evaluations should examine how human behavior changes due to external assistance when using these control models.
A novel resilience analysis methodology for airport networks system from the perspective of different epidemic prevention and control policy responses
Authors: Guo Jiuxia , Yang Zongxin , Zhong Qingwei , Sun Xiaoqian , Wang Yinhai
Published: February 2023

As the COVID-19 pandemic fades, the aviation industry is entering a fast recovery period. To analyze airport networks’ post-pandemic resilience during the recovery process, this paper proposes a Comprehensive Resilience Assessment (CRA) model approach using the airport networks of China, Europe, and the U.S.A as case studies. The impact of COVID-19 on the networks is analyzed after populating the models of these networks with real air traffic data. The results suggest that the pandemic has caused damage to all three networks, although the damages to the network structures of Europe and the U.S.A are more severe than the damage in China. The analysis suggests that China, as the airport network with less network performance change, has a more stable level of resilience. The analysis also shows that the different levels of stringency policy in prevention and control measures during the epidemic directly affected the recovery rate of the network. This paper provides new insights into the impact of the pandemic on airport network resilience.
The impact of adjuvant chemotherapy on survival in mucinous and non-mucinous rectal adenocarcinoma patients after TME surgery
Authors: Vernmark Karolina , Knutsen Annika , Loftås Per , Sun Xiao-Feng
Published: February 2023

Introduction

The value of adjuvant chemotherapy for rectal cancer patients is debated and varies in different subgroups. One such subgroup is mucinous adenocarcinoma (MAC), which is more treatment resistant compared to non-mucinous adenocarcinoma (NMAC). To date, mucinous histology is not taken into account when deciding on adjuvant treatment strategy. This is the first study to exclusively include patients with rectal cancer, then separate MAC and NMAC and compare the survival in patients that had or did not have adjuvant chemotherapy.

Material and methods

The study included retrospective register data from 365 Swedish patients with stage II-IV rectal adenocarcinoma, 56 patients with MAC and 309 patients with NMAC. All patients were considered curative, had surgery with total mesorectal excision in 2004–2013, and were followed up until death or 2021.

Results

Patients with MAC that had adjuvant chemotherapy had better overall survival (OS, HR 0.42; CI 95%: 0.19–0.93;p= 0.032) and a trend towards better cancer-specific survival (CSS, HR 0.41 CI 95%: 0.17–1.03;p= 0.057) compared to patients without chemotherapy (HR 0.42; CI 95%: 0.19–0.93;p= 0.032). The difference in OS was still significant even after adjusting for sex, age, stage, differentiation, neoadjuvant chemotherapy and preoperative radiotherapy (HR 0.40; CI 95%: 0.17–0.92;p= 0.031). There was no such difference in the NMAC patients except in the stage-by-stage subgroup analyses where patients in stage IV had better survival after adjuvant chemotherapy.

Conclusions

There may be a difference in treatment response to adjuvant chemotherapy between MAC and NMAC patients. Patients with MAC could possibly benefit from adjuvant chemotherapy in stages II-IV. Further studies are however needed to confirm these results.
Multidrug resistant Klebsiella Pneumoniae reservoir and their capsular resistance genes in cow farms of district Peshawar, Pakistan
Authors: Saddam Saddam , Khan Muddasir , Jamal Muhsin , Rehman Sadeeq Ur , Slama Petr , Horky Pavel
Published: February 2023

Klebsiella pneumoniae is a major zoonotic pathogen that causes a variety of severe illnesses as well as mastitis. The distribution of mastitis-causing K. Pneumoniae and its virulence factors vary by country and geographical location. The present study aimed to find out the occurrence of Multidrug-resistant (MDR) K. Pneumoniae and their capsular resistance genes which were undocumented previously in cow farms of district Peshawar, Pakistan. A total of 700 milk samples from symptomatic mastitic cows were screened for MDR K. Pneumoniae. Furthermore, the characterization of capsular resistance genes was done by molecular techniques. Among these samples, K. pneumoniae was found 180/700 (25.7%), while MDR K. pneumoniae was found 80/180 (44.4%). The antibiogram analysis revealed high resistance to Vancomycin (95%) while highly sensitive to Ceftazidime (80%). The distribution of capsular genes shows the most common serotype K2 gene 39/80 (48.7%), followed by serotype K1 gene 34/80 (42.5%), serotype K5 17/80 (21.2%), and serotype K54 13/80 (16.2), respectively. Moreover, the co-occurrence of serotypes K1+K2 was found at 11.25%, KI+K5 was 05%, K1+K54 was 3.75%, and K2+K5 was 7.5%, respectively. A statistically significant association (p ≤ 0.05) was found between predicted and discovered K. pneumoniae values. In conclusion, the presence of MDR K. pneumoniae in combination with capsular genes may be a possible threat to dairy farm animals and humans in Peshawar, Pakistan. It may give us special attention to follow up on hygienic practices in livestock management.
Numerical simulation of periodic surface structures created by direct laser interference patterning
Authors: Heinrich Martin , Voisiat Bogdan , Lasagni Andrés Fabián , Schwarze Rüdiger
Published: February 2023

Surface structuring using nano-second lasers can be used to enhance certain properties of a material or even to introduce new ones. One way to create these structures efficiently is direct laser interference patterning using different polarization vector orientations of the interfering beams. However, experimentally measuring the fabrication process of these structures is very challenging due to small length and time scales. Therefore, a numerical model is developed and presented for resolving the physical effects during formation the predicting the resolidified surface structures. This three-dimensional, compressible computational fluid dynamics model considers the gas, liquid, and solid material phase and includes various physical effects, such as heating due to the laser beam for both parallel and radial polarization vector orientations, melting, solidification, and evaporation, Marangoni convection, and volumetric expansion. The numerical results reveal a very good qualitatively and quantitatively agreement with experimental reference data. Resolidified surface structures match both in overall shape as well as crater diameter and height, respectively. Furthermore, this model gives valuable insight on different quantities during the formation of these surface structures, such as velocity and temperature. In future, this model can be used to predict surface structures based on various process input parameters.
Estimation of patient flow in hospitals using up-to-date data. Application to bed demand prediction during pandemic waves
Authors: Garcia-Vicuña Daniel , López-Cheda Ana , Jácome María Amalia , Mallor Fermin
Published: February 2023

Hospital bed demand forecast is a first-order concern for public health action to avoid healthcare systems to be overwhelmed. Predictions are usually performed by estimating patients flow, that is, lengths of stay and branching probabilities. In most approaches in the literature, estimations rely on not updated published information or historical data. This may lead to unreliable estimates and biased forecasts during new or non-stationary situations. In this paper, we introduce a flexible adaptive procedure using only near-real-time information. Such method requires handling censored information from patients still in hospital. This approach allows the efficient estimation of the distributions of lengths of stay and probabilities used to represent the patient pathways. This is very relevant at the first stages of a pandemic, when there is much uncertainty and too few patients have completely observed pathways. Furthermore, the performance of the proposed method is assessed in an extensive simulation study in which the patient flow in a hospital during a pandemic wave is modelled. We further discuss the advantages and limitations of the method, as well as potential extensions.
Sociometric network analysis in illicit drugs research: A scoping review
Authors: Zakimi Naomi , Greer Alissa , Bouchard Martin , Dhillon Arshpreet , Ritter Alison
Published: February 2023

Background

Sociometric or whole network analysis, a method used to analyze relational patterns among social actors, emphasizes the role of social structure in shaping behaviour. Such method has been applied to many aspects of illicit drug research, including in the areas of public health, epidemiology, and criminology. Previous reviews about social networks and drugs have lacked a focus on the use of sociometric network analysis for illicit drugs research across disciplines. The current scoping review aimed to provide an overview of the sociometric network analysis methods used in illicit drugs research and to assess how such methods could be used for future research.

Methods

A systematic search of six databases (Web of Science, ProQuest Sociology Collection, Political Science Complete, PubMed, Criminal Justice Abstracts, and PsycINFO) returned 72 relevant studies that met the inclusion criteria. To be included, studies had to mention illicit drugs and use whole social network analysis as one of their methods. Studies were summarized quantitatively and qualitatively using a data-charting form and a description of the studies’ main topics.

Results

Sociometric network analysis in illicit drugs research has grown in popularity in the last decade, using mostly descriptive network metrics, such as degree centrality (72.2%) and density (44.4%). Studies were found to belong to three study domains. The first, drug crimes investigated network resilience and collaboration patterns in drug trafficking networks. The second domain, public health, focused on the social networks and social support of people who use drugs. Finally, the third domain focused on the collaboration networks of policy, law enforcement, and service providers.

Conclusion

Future illicit drugs research using whole network SNA should include more diverse data sources and samples, incorporate mixed and qualitative methods, and apply social network analysis to study drug policy.
Strategy and additive technologies as the catalyst for outsourcing, process innovation and operational effectiveness
Authors: Tegethoff Thomas , Santa Ricardo , Cayón Edgardo , Scavarda Annibal
Published: February 2023

Purpose

There is rising interest in Industry 4.0 as a factor in the competitiveness of the organization. Although many companies are aware of the importance of Industry 4.0, the development of such initiatives in Colombia is slow. Consequently, this research investigates the impact of additive technologies as part of the Industry 4.0 concept on operational effectiveness and, therefore, the competitiveness of the organization and tries to establish the factors that hinder the adequate implementation of such new, innovative technologies.

Design/Methodology/Approach

Structural equation modeling was used to analyze the antecedents and outcomes of operational effectiveness. To this end, 946 usable questionnaires were collected from managers and personnel from Colombian organizations.

Findings

Initial findings show that management is aware of Industry 4.0 concepts and implements strategies for such initiatives. Nevertheless, neither process innovation nor additive technologies have a significant impact on operational effectiveness and therefore on the competitiveness of the organization.

Practical implications

The implementation of new innovative technologies requires the closure of the digital gap between urban and rural areas and between large and medium and small enterprises. Similarly, the concept of Industry 4.0 as a new, innovative manufacturing concept requires a transversal implementation to increase the competitiveness of the organization.

Originality/Value

The value of this paper lies in discussing the current technological and human capabilities and strategies that Colombian organizations, as an example of a developing nation, should improve to leverage the benefits of Industry 4.0 to remain competitive. The results are probably generalizable to other regions in developing countries throughout the world.
The metabolic profile of waist to hip ratio–A multi-cohort study
Authors: Lind Lars , Ahmad Shafqat , Elmståhl Sölve , Fall Tove
Published: February 2023

Background

The genetic background of general obesity and fat distribution is different, pointing to separate underlying physiology. Here, we searched for metabolites and lipoprotein particles associated with fat distribution, measured as waist/hip ratio adjusted for fat mass (WHRadjfatmass), and general adiposity measured as percentage fat mass.

Method

The sex-stratified association of 791 metabolites detected by liquid chromatography–mass spectrometry (LC-MS) and 91 lipoprotein particles measured by nuclear magnetic spectroscopy (NMR) with WHRadjfatmass and fat mass were assessed using three population-based cohorts: EpiHealth (n = 2350) as discovery cohort, with PIVUS (n = 603) and POEM (n = 502) as replication cohorts.

Results

Of the 193 LC-MS-metabolites being associated with WHRadjfatmass in EpiHealth (false discovery rate (FDR) <5%), 52 were replicated in a meta-analysis of PIVUS and POEM. Nine metabolites, including ceramides, sphingomyelins or glycerophosphatidylcholines, were inversely associated with WHRadjfatmass in both sexes. Two of the sphingomyelins (d18:2/24:1, d18:1/24:2 and d18:2/24:2) were not associated with fat mass (p>0.50). Out of 91, 82 lipoprotein particles were associated with WHRadjfatmass in EpiHealth and 42 were replicated. Fourteen of those were associated in both sexes and belonged to very-large or large HDL particles, all being inversely associated with both WHRadjfatmass and fat mass.

Conclusion

Two sphingomyelins were inversely linked to body fat distribution in both men and women without being associated with fat mass, while very-large and large HDL particles were inversely associated with both fat distribution and fat mass. If these metabolites represent a link between an impaired fat distribution and cardiometabolic diseases remains to be established.
The use of virtual reality in screening for preclinical Alzheimer’s disease: A scoping review protocol
Authors: Tian Yuan , Kuruvilla Maneesh V. , Park Mira
Published: February 2023

Introduction

Preclinical Alzheimer’s disease (AD) represents the earliest phase of AD, often years before the onset of mild cognitive impairment (MCI). There is a pressing focus on identifying individuals in the preclinical AD phase to alter the trajectory or impact of the disease potentially. Increasingly, Virtual Reality (VR) technology is being used to support a diagnosis of AD. While VR technology has been applied to the assessment of MCI and AD, studies about how best to utilize VR as a screening tool for preclinical AD are limited and discordant. The objectives of this review are to synthesize the evidence pertaining to the use of VR as a screening tool for preclinical AD as well as to identify factors that need to be considered when utilizing VR to screen for preclinical AD.

Methods and analysis

The methodological framework proposed by Arksey and O’Malley (2005) will be introduced to guide the conduction of the scoping review, and Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for scoping reviews (PRISMA-ScR) (2018) will be used to organize and structure the review. PubMed, Web of Science, Scopus, ScienceDirect and Google Scholar will be used to search for literature. Obtained studies will be screened for eligibility based on predefined exclusion criteria. A narrative synthesis of eligible studies will be performed, after tabulating the extracted data from existing literature, to answer the research questions.

Ethics and dissemination

Ethical approval is not required for this scoping review. Findings will be disseminated through conference presentations, publication in a peer-reviewed journal, and discussions among professional networks in the research domain combining neuroscience and information and communications technology (ICT).

Registration details

This protocol has been registered on Open Science Framework (OSF). Relevant materials and potential following updates are available at https://osf.io/aqmyu.
Evaluation of calculation processes of apparent diffusion coefficient subtraction method (ASM) imaging
Authors: Barham Majd , Kuroda Masahiro , Yoshimura Yuuki , Hamada Kentaro , Khasawneh Abdullah , Sugimoto Kohei , Konishi Kohei , Tekiki Nouha , Sugianto Irfan , Bamgbose Babatunde O. , Ishizaka Hinata , Shimizu Yudai , Nakamitsu Yuki , Al-Hammad Wlla E. , Kamizaki Ryo , Kurozumi Akira , Matsushita Toshi , Ohno Seiichiro , Asaumi Junichi
Published: February 2023

A number of restricted diffusion (RD) imaging techniques, such as diffusion kurtosis (DK) imaging and Q space imaging, have been developed and proven to be useful for the diagnosis of diseases, including cerebral gliomas and cerebrovascular infarction. In particular, apparent diffusion coefficient (ADC) subtraction method (ASM) imaging has become available recently as a novel RD imaging technique. ASM is based on the difference between the ADC values in an image pair of two ADC maps, ADC basic (ADCb) and ADC modify (ADCm), which are created from diffusion-weighted images taken using short and long effective diffusion times, respectively. The present study aimed to assess the potential of different types of ASM imaging by comparing them with DK imaging which is the gold-standard RD imaging technique. In the present basic study using both polyethylene glycol phantom and cell-containing bio-phantom, three different types of ASM images were created using different calculation processes. ASM/A is an image calculated by dividing the absolute difference between ADCb and ADCm by ADCb several times. By contrast, ASM/S is an image created by dividing the absolute difference between ADCb and ADCm by the standard deviation of ADCb several times. As for positive ASM/A image (PASM/A), the positive image, which was resultant after subtracting ADCb from ADCm, was divided by ADCb several times. A comparison was made between the types of ASM and DK images. The results showed the same tendency between ASM/A in addition to both ASM/S and PASM/A. By increasing the number of divisions by ADCb from three to five times, ASM/A images transformed from DK-mimicking to more RD-sensitive images compared with DK images. These observations suggest that ASM/A images may prove useful for future clinical applications in RD imaging protocols for the diagnosis of diseases.
Capsid structure of a fungal dsRNA megabirnavirus reveals its previously unidentified surface architecture
Authors: Wang Han , Salaipeth Lakha , Miyazaki Naoyuki , Suzuki Nobuhiro , Okamoto Kenta
Published: February 2023

Rosellinia necatrix megabirnavirus 1-W779 (RnMBV1) is a non-enveloped icosahedral double-stranded (ds)RNA virus that infects the ascomycete fungus Rosellinia necatrix, a causative agent that induces a lethal plant disease white root rot. Herein, we have first resolved the atomic structure of the RnMBV1 capsid at 3.2 Å resolution using cryo-electron microscopy (cryo-EM) single-particle analysis. Compared with other non-enveloped icosahedral dsRNA viruses, the RnMBV1 capsid protein structure exhibits an extra-long C-terminal arm and a surface protrusion domain. In addition, the previously unrecognized crown proteins are identified in a symmetry-expanded cryo-EM model and are present over the 3-fold axes. These exclusive structural features of the RnMBV1 capsid could have been acquired for playing essential roles in transmission and/or particle assembly of the megabirnaviruses. Our findings, therefore, will reinforce the understanding of how the structural and molecular machineries of the megabirnaviruses influence the virulence of the disease-related ascomycete fungus.
The alphavirus nonstructural protein 2 NTPase induces a host translational shut-off through phosphorylation of eEF2 via cAMP-PKA-eEF2K signaling
Authors: Treffers Emmely E. , Tas Ali , Scholte Florine E. M. , de Ru Arnoud H. , Snijder Eric J. , van Veelen Peter A. , van Hemert Martijn J.
Published: February 2023

Chikungunya virus (CHIKV) is a reemerging alphavirus. Since 2005, it has infected millions of people during outbreaks in Africa, Asia, and South/Central America. CHIKV replication depends on host cell factors at many levels and is expected to have a profound effect on cellular physiology. To obtain more insight into host responses to infection, stable isotope labeling with amino acids in cell culture and liquid chromatography-tandem mass spectrometry were used to assess temporal changes in the cellular phosphoproteome during CHIKV infection. Among the ~3,000 unique phosphorylation sites analyzed, the largest change in phosphorylation status was measured on residue T56 of eukaryotic elongation factor 2 (eEF2), which showed a >50-fold increase at 8 and 12 h p.i. Infection with other alphaviruses (Semliki Forest, Sindbis and Venezuelan equine encephalitis virus (VEEV)) triggered a similarly strong eEF2 phosphorylation. Expression of a truncated form of CHIKV or VEEV nsP2, containing only the N-terminal and NTPase/helicase domains (nsP2-NTD-Hel), sufficed to induce eEF2 phosphorylation, which could be prevented by mutating key residues in the Walker A and B motifs of the NTPase domain. Alphavirus infection or expression of nsP2-NTD-Hel resulted in decreased cellular ATP levels and increased cAMP levels. This did not occur when catalytically inactive NTPase mutants were expressed. The wild-type nsP2-NTD-Hel inhibited cellular translation independent of the C-terminal nsP2 domain, which was previously implicated in directing the virus-induced host shut-off for Old World alphaviruses. We hypothesize that the alphavirus NTPase activates a cellular adenylyl cyclase resulting in increased cAMP levels, thus activating PKA and subsequently eukaryotic elongation factor 2 kinase. This in turn triggers eEF2 phosphorylation and translational inhibition. We conclude that the nsP2-driven increase of cAMP levels contributes to the alphavirus-induced shut-off of cellular protein synthesis that is shared between Old and New World alphaviruses. MS Data are available via ProteomeXchange with identifier PXD009381.
A spinner of yarns: Conversations with Auntie Mady
Authors: Minier Márta
Published: February 2023

This article reflects on an oral history interview about expatriate life and transcultural belonging with lifelong traveller and multiple immigrant Mady Gerrard and reads Gerrard’s own rich and illuminating life writing, a memoir entitled Full Circle (). Having survived the Holocaust as a teenage girl, Gerrard returned to Hungary only to realize that the Soviet-enforced Communist regime was just another dictatorship. She left the country with her 3-year-old daughter, along with many other political refugees, in 1956 and settled in Wales, which she exchanged later (mostly in response to professional challenges) for Canada and the United States but returned to her adopted home, Wales, for her mature years. Our conversations were cut short by the COVID-19 lockdowns and then Mady’s death in the autumn of 2021, but the article attempts to channel her vivacious spirit and life-affirming wisdom and contextualize her legacy as important both to the memory of the Holocaust and as a contemporary woman’s material/tangible and intangible legacy (encompassing her handicraft work, writing and public appearances). The contribution consists of a framing piece of academic writing and a more creative and somewhat (auto-)ethnographic vignette capturing our encounters.
Structural Analysis of Calcium Oxide Derived from Waste Egg’s
Shells and their Application for Knoevenagel Condensation Reactions
Authors: Rathod S.B. , Pawar N.M. , Shirke P.M. , Shinde B.L. , Dhage V.N.
Published: February 2023

The present study demonstrates the simple and easy synthesis of calcium oxide (CaO) from waste
egg’s shells. Fine egg’s shells powder were calcinated at 500, 700 and 800 ºC. The structural and
morphological evaluation of calcium oxide derived from waste egg’s shell were examined by using
XRD, FT-IR, SEM, EDS and TEM techniques. The sintered calcium oxide was used as heterogeneous
catalyst for the synthesis of 5-arylidene malononitrile and 5-arylidene barbituric acid derivatives by
the condensation of various aromatic aldehyde and active methylene compounds via Knoevenagel
condensations reaction under ultrasound irradiation method. The reaction completed within a very
short reaction time with high yields of product. The present method describes easy synthesis of calcium
oxide catalyst from the waste egg’s shells, short reaction times, simple product isolation, excellent
yield and reusability of the catalyst.
Mobilfunk ja – aber Masten und Antennen bitte nicht hier! Diskursive Aushandlungsprozesse um die Standortwahl von Sendeanlagen in Deutschland
Authors: Dittel Julia , Weber Florian , Berr Karsten , Kühne Olaf
Published: February 2023

A stable and nationwide mobile network coverage is increasingly regarded as essential. This requires an expansion of mobile networks throughout the country. However, the installation of masts and antennas has often been the subject of conflict in Germany, especially due to fears about potential health effects of electromagnetic fields. Transmitters become both a trigger and a target of social protests. From a discourse-theoretical perspective, we identify central lines of argumentation in the German cellular network conflicts. A triangulation of quantitatively oriented and qualitative analysis components forms the methodological basis. Regional case studies provide an opportunity to identify local differences. A comparison of past and recent conflicts reveals discursive shifts over time. Debates about the possible health effects of mobile radio were particularly contentious in the context of UMTS mobile phone expansion at the turn of the millennium. The (planned) installation of transmitters at the local level often sparked this discussion. During the expansion of the 5G network, in addition to the debate about the health risks of the technology, questions are now being raised about whether and to whom the expansion is necessary. Compared to the previous period of investigation, local conflicts about transmitters now appear to be less virulent and can be found primarily in southern and southwestern Germany.
DISTINCTIVE COLLECTIVIST IDENTITY ON DIGITAL ADS: HARI RAYA AND LEBARAN 2021
Authors: Marta Rustono Farady , Supina Supina , Rashid Sabrina Mohd , Karolina Cut Meutia , Naseer Moazzam
Published: February 2023

Every family has a particular moment, especially in Malaysia and Indonesia, where the majority of the population is Muslim, where Eid al-Fitr has established a tradition for bringing family members closer together. Simply said, the tradition is known as "Hari Raya” whereas, in Indonesia, the term "Lebaran" is used. The year 2021 will be remembered for the social restraints put in place to prevent the spread of COVID-19. This study aims to capture this once-a-year event, which will then be interpreted using Oswald's Marketing Semiotics strategy to juxtapose the two digital advertising media on the Youtube Channel for cellular operator brands in each country in the Interpretivism Paradigm using a qualitative approach based on Hofstede's Cultural Dimension Theory. The Hari Raya 2021 Maxis advertisement symbolizes the State of Malaysia, while the 2021 Lebaran advertisement from the Indosat Ooredoo Provider represents the State of Indonesia, both of which are displayed in binary opposition. The study's findings reveal a consistent pattern in promoting the country's collectivist identity, with marketing distinctiveness arising from a variety of key components. The dominant color is green, which has a characteristic Malaysian Muslim nuance in its advertising storyline with the theme "Raya Si Sakan," which represents the Malaysian Cultural Dimension of Short-Term Orientation and Masculinity. In contrast to the majority of the color yellow, the title "Bulan yang Baik," reflects the warmth of friendship in terms of the identical dimensions of Indonesian culture, which tends towards long-term orientation and femininity in terms of the majority of the color yellow.
The Changing and Growing Roles of Independent Central Banks Now Do Require a Reconsideration of Their Mandate
Authors: Goodhart Charles , Lastra Rosa
Published: February 2023

In this paper, we analyse why the changing and growing roles of independent Central Banks now do require a reconsideration of their mandate.