bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of chondrogenic potential of human dermal fibroblasts after modification with differentiation media and cytokine TGF-β3
Authors: Bozhokin Mikhail S. , Marchenko Daria M. , Mikhaylova Elena R. , Rakhimov Bulat R. , Bozhkova Svetlana A. , Korneva Yulia S. , Aleksandrova Svetlana A. , Khotin Mikhail G.
Published: September 2024

BACKGROUND: Hyaline cartilage is an avascular tissue that envelopes the surface of major joints. The regenerative capacity of this tissue is restricted due to its high content of extracellular matrix proteins and its modest number of cells. Articular cartilage recovery remains relevant and yet unresolved to date. Utilizing biomedical products that contain modified cells is a promising approach.
AIM: To examine the effects of various culture media on the chondrogenic modification of cells and to compare the results.
MATERIALS AND METHODS: Human dermal fibroblasts and rabbit multipotent mesenchymal stromal cells were modified using the chondrogenic differentiation medium StemPro or recombinant TGF-β3 protein. Alterations in the expression of genes involved in chondrogenesis (Acan, Tgfβ3, Col2α1, Comp) were assessed using the real-time polymerase chain reaction method ΔΔCt.
RESULTS: It was demonstrated that human dermal fibroblasts can induce chondrogenic modification when used in protein media. This cell type is easy to harvest, does not necessitate special cultivation conditions, is readily scalable, and is suitable for allogeneic transplantation.
CONCLUSION: The obtained data can be employed to develop tissue engineering products for the regeneration of hyaline cartilage using allogeneic dermal fibroblasts.
Haloperidol-induced experimental osteoporosis and its correction with dimephosphone
Authors: Ilyina Roza Ju. , Muchamedzhanova Lubov R. , Ziganshina Liliya E.
Published: September 2024

BACKGROUND: There are reports of the negative impact of neuroleptics long-term use on bone metabolism. Dimephosphone is a drug with proven antirachitic effect.
AIM: To study under experimental conditions the effect of haloperidol on the microelement composition of the bone of experimental rats and the possibilities of its correction with dimephosphone.
MATERIAL AND METHODS: Wistar rats were divided into groups (10 animals in each): the first was the control group; the second group consisted of animals that were administered haloperidol; the third group used haloperidol with dimephosphone. Rats of the second and third groups were administered haloperidol intraperitoneally for 90 days, starting with 15 mg/day and reducing the dose to 5 mg/day. Rats of the third group were administered orally a 15% dimephosphone solution at a dose of 208 mg/kg/day. After the animals were withdrawn from the experiment, the lumbar vertebrae were dissected and the teeth were removed. The content of calcium, magnesium, strontium, zinc and copper ions in bone and teeth was determined by atomic absorption spectrophotometry. Statistical processing was performed using the SPSS 13.0 program.
RESULTS: Long-term use of therapeutic doses of haloperidol in the experiment caused a decrease in the content of calcium and zinc ions by 1.5 times, copper — by 1.7 times in the bones of animals, an increase in the concentration of strontium ions by 1.1 times, which indicated the resorptive effect of the neuroleptic. With the combined use of haloperidol and dimephosphone, the content of calcium ions (by 1.2 times), copper (by 1.4 times) and zinc ions (by 1.1 times) significantly increased; the amount of strontium ions decreased (by 1.1 times) only in bone tissue.
CONCLUSION: Dimephosphone reduces the negative effect of long-term haloperidol administration on the microelement composition of rat bones.
EFISIENSI BENTUK RANGKA JEMBATAN DENGAN KOMBINASI TIPE WARREN DAN K-TRUSS MATERIAL HOLLOW BJ 37
Authors: Sulton Mohammad , Al Ansyorie Mohammad Musthofa , Ramadhan Defa Gilang
Published: September 2024

Abstrak : Kekuatan struktur jembatan menjadi hal utama yang harus diperhatikan mengingat pembangunan sebuah jembatan berfungsi untuk menahan beban yang bekerja pada jembatan. Salah satu hal yang berpengaruh terhadap kekuatan struktur jembatan rangka adalah pemilihan bentuk rangka jembatan. Jembatan dengan tipe k-truss dan warren memiliki kelebihan pada berat struktur dan kekuatan jembatan. Jembatan rangka memiliki nilai yang paling efisien apabila memiliki nilai lendutan dan berat paling minimum. Metode yang dilakukan dengan cara menganalisis bentuk rangka dengan kombinasi tipe k-truss dan warren, kemudian memodifikasi serta memberikan konfigurasi breaching untuk mendapatkan satu bentuk rangka terbaik ditinjau dari nilai lendutan dan berat jembatan. Kemudian membuat benda uji jembatan model untuk dilakukan uji pembebanan statis secara analisis dan uji model. Kekuatan struktur jembatan ditinjau dari nilai lendutan dan berat struktur jembatan. Nilai efisiensi model jembatan dihitung dengan mengalikan nilai lendutan dan berat jembatan. Jembatan yang paling efisien apabila memiliki nilai efisiensi terkecil. Berdasarkan hasil analisis menggunakan program aplikasi SAP 2000 didapat satu bentuk rangka yang memiliki nilai efisiensi terbaik sebesar 0,0652 yaitu pada rangka kode KF 5. Ukuran rangka jembatan KF 5 memiliki panjang segmen bentang ke-1 sampai ke-5 secara berurutan 550 mm, 700 mm, 500 mm, 700 mm, dan 550 mm.. Nilai lendutan pada rangka hasil optimasi akibat beban stastis pada ½ bentang secara analisis sebesar 0,3412 mm sedangkan secara uji model sebesar 0,5275 mm.
PENGEMBANGAN WEBSITE PAWANUDO BERBASIS CARRD.CO SEBAGAI MEDIA PEMBELAJARAN SEJARAH PERJUANGAN FISIK PAHLAWAN NASIONAL PEREMPUAN INDONESIA
Authors: Sulistiowati Irma , Fitria Utama Hari , Ningtyas Dwi Wahyu , Zaky Azymah Muhammad
Published: September 2024

Penelitian ini bertujuan untuk mengembangkan media pembelajaran Sejarah berupa website Pawanudo berbasis Carrd.co serta menguji efektivitas produk pengembangan melalui beberapa uji coba. Metode penelitian dan pengembangan ini menggunakan model milik Sugiyono (2016) dengan tahap sebagai berikut, (1) analisa potensi masalah, (2) pengumpulan data, (3) desain produk, (4) validasi desain, (5) revisi desain, (6) uji coba produk, (8) revisi produk, (9) uji coba pemakaian, (10) revisi produk, (11) produksi massal. Hasil validasi materi diperoleh persentase sebesar 93 persen sedangkan hasil validasi media memperoleh persentase sebesar 85 persen. Hasil uji coba kelompok kecil sebesar 70 persen sedangkan hasil uji kelompok besar memperoleh persentase 84 persen. Berdasarkan data validasi dan hasil uji coba dapat dinyatakan kesimpulan sebagai berikut (1) Proses penelitian dan pengembangan dapat dilaksanakan dengan baik sesuai dengan analisa pada tahap awal. (2) Efektivitas website Pawanudo secara keseluruhan masuk dalam kategori efektif (3) Respon peserta didik dan perolehan hasil evaluasi terbilang sangat baik. Website Pawanudo memiliki potensi untuk dikembangkan lebih lanjut sebagai media pembelajaran sejarah dengan mengusung materi yang berbeda.
Supporting Students’ Physical Activity Self-Efficacy within High School Physical Education
Authors: Murfay Ken , Beighle Aaron , Erwin Heather , Aiello Erin , Pyszynski Sarah
Published: September 2024

Research examining physical activity (PA) is significant because of the known benefits of PA. Studying high school (HS) students is particularly important due to the consistent decline in PA as students age. One strategy to increase PA is to increase someone’s self-efficacy (confidence) to be physically active which is commonly positively associated with PA. Most of the research on how to support physical activity self-efficacy (PASE) comes from outside-of-school interventions with minimal literature on how to support students’ PASE within HS physical education (PE). This study attempted to identify and share practices that current HS PE teachers were implementing to support students’ PASE and further develop previous recommendations to support PASE. The study participants were 14 current HS PE teachers that participated in one individual semi-structured interview that lasted approximately 50-60 minutes. Interview questions were designed to ask participants about their daily in-person class routines and how they support PASE based on the four sources of self-efficacy. The results include three themes to assist in supporting HS students’ PASE which include: (a) Maximizing initial success, (b) Redefining success, and (c) Increasing PA awareness and monitoring. All themes and examples coincide with previous recommendations on how best to support PASE. PE teachers have the opportunity to design PA experiences that maximize the chances of students interpreting their experiences as successful and allow students to manage their PA participation. Implementing these types of strategies can hopefully increase students’ overall participation in PA by supporting their PASE.
A Study on the Reading-Writing Combined English Instruction in Junior High School Based on Thematic Contexts
Authors: Yu Danxia
Published: September 2024

Context is an effective way for students to master English and a crucial method to stimulate their interest in learning. This paper tries to explore a teaching strategy that combines reading and writing in English based on thematic contexts through practical teaching cases. The first step is to activate students’ background information by creating thematic contexts; the second step is to integrate necessary textual information for writing around thematic contexts; the next step is to explore thematic contexts to broaden students’ reading perspectives and overcome their limitations in thinking; the last step is to apply thematic contexts to improve the comprehensive use of reading and writing skills. The reading-writing combined English instruction based on thematic contexts can achieve the goal of coordinated and high-quality development of students’ English reading and writing abilities.
EMBA Practical Teaching Case Analysis—Taking Private Board of Directors as an Example
Authors: Zhang Bowen , Mei Shiqi , Ti Haowei , Huang Liang
Published: September 2024

Executive Master of Business Administration (EMBA) is the cradle of entrepreneurs and professional managers. As founders, entrepreneurs have been in a homogeneous environment for a long time. In addition to understanding the companies they founded, they don’t know as much about other companies as professional managers, and in the private board of directors, they can see the outside world. First of all, Private Board of Directors is different from traditional business school education. The teaching model of Private Board of Directors makes learning more interactive. Traditional business school education is standardized and focuses on knowledge logic. But private board meetings are personalized, and learning from each other puts more emphasis on self-perception. Private boards are organized differently. Since the private board of directors has strict control over the size of the group, has strict requirements for member qualifications, and has a complete set of activity rules, the longer it takes, the longer it will take. The longer the students stay together, the more in-depth the communication between the private board members will be. Entrepreneurs, as leaders, are rarely challenged within the company. But the private board of directors is different. All members are equal. The practical teaching principle of Hong Kong Asia Business College’s EMBA is to fully unleash the effectiveness of existing models and innovative models.
Comparison of Four Automatic Detection Algorithm with Initial Parameters for DICOM Artificial Brain Shift
Authors: Watanabe Kaoru , Mori Takumi , Noborio Hiroshi , Koeda Masanao
Published: September 2024

In neurosurgery to remove brain tumors, DICOM data, a medical imaging standard, is generated preoperatively using CT and MRI. This data is used for surgical planning. However, brain deformities, known as brain shifts, can occur during surgery and deviate from the preoperative surgical plan. Deviations from the surgical plan due to brain shift are a life-threatening problem as they reduce the success rate of surgery. Brain shift has not yet been elucidated and DICOM data acquired by MRI and CT for surgical planning and post-operative management are stored and archived at hospitals and discarded after a certain period. To address these issues, we started research around 2018 with the goal of modelling brain shifts. This will enable surgical planning to take brain shifts into account during pre-operative conferences. The corresponding OpenCV feature points are extracted from the pre-operative and post-operative DICOM and the brain shift is extracted from their motion vectors. Here, the feature point extraction algorithms BRISK, AKAZE, ORB, and SIFT are compared and it is experimentally confirmed that BRISK and AKAZE have better brain shift extraction capability than the other two algorithms.
Peran Pengalaman sebagai Pereduksi Bias Heuristik dan Herding Effect dalam Keputusan Investasi
Authors: Praditha Riza , Bahtiar Bahtiar , Razak Linda Arisanty
Published: September 2024

Latar Belakang: Pengambilan keputusan investasi tidak selalu didasari oleh analisis fundamental, bahkan umumnya investor dapat bertindak irrasional dalam mengambil keputusan. Hal ini yang mengakibatkan timbulnya anomali di pasar investasi. Tujuan: Penelitian ini bertujuan untuk menguji pengaruh faktor heuristic bias keterwakilan dan herding effect terhadap keputusan investasi, serta peran pengalaman sebagai pereduksi.Metode Penelitian: Objek penelitian ini adalah persepsi perilaku investor emas (logam mulia) terhadap keputusannya membeli emas. Metode analisis yang digunakan adalah regresi linier berganda dan Uji Sampel Berpasangan.Hasil Penelitian: Hasil penelitian menunjukkan bahwa bias heuristik keterwakilan terbukti mempengaruhi keputusan investor dalam membeli emas, sedangkan herding effect tidak menunjukkan pengaruh yang signifikan terhadap keputusan membeli emas.Keaslian/Kebaruan Penelitian: Temuan menarik dalam penelitian ini adalah pengalaman investor terbukti berperan dalam mengurangi bias keputusan yang terjadi akibat heuristic bias dan herding effect.
Determinan Return Saham dengan Profitabilitas sebagai Variabel Intervening
Authors: Hadiwibowo Imam , Saraswati Erwin
Published: September 2024

Latar Belakang: Di tengah krisis pandemi COVID-19, jumlah investor yang tertarik berinvestasi dengan modal minim meningkat, meskipun sering tanpa melakukan riset yang mendalam. Kemajuan teknologi dan perkembangan pesat pasar modal, yang difasilitasi oleh banyaknya platform transaksi online, memudahkan proses investasi yang mendorong lebih banyak orang untuk berinvestasi, yang potensial berkontribusi pada kemajuan ekonomi negara. Investasi dianggap sebagai strategi penting untuk memenuhi kebutuhan masa depan dan mencari keuntungan. Tujuan: Tujuan dari penelitian ini adalah untuk menguji dan mendapatkan bukti empiris pengaruh Debt to Equity Ratio (DER) dan Earning per Share (EPS) terhadap return saham, dan mempertimbangkan variabel profitabilitas sebagai variabel Intervening.Metode Penelitian: Penelitian ini menggunakan sampel 50 perusahaan makanan dan minuman dan Perusahaan Industri Rokok dari tahun 2020 – 2023. Data Penelitian ini diolah menggunakan alat analisis eviews12. Analisis data yang digunakan adalah statistik deskriptif, uji asumsi klasik, uji koefisien determinasi, dan path analysis.Hasil Penelitian: Hasil Penelitian menunjukkan bahwa DER dan EPS tidak berpengaruh terhadap return saham, DER berpengaruh negatif terhadap profitabilitas, EPS berpengaruh positif terhadap profitabilitas, profitabilitas tidak berpengaruh terhadap return saham, dan profitabilitas tidak mempu memediasi pengaruh DER dan EPS terhadap return saham perusahaan makanan dan minuman dan perusahaan sektor rokok tahun 2020 – 2023.Keaslian/Kebaruan Penelitian: Kebaruan penelitian ini terletak pada analisis bagaimana profitabilitas perusahaan berperan sebagai variabel intervening antara berbagai determinan dengan return saham. Penelitian yang menggabungkan variabel intervening ini dapat memberikan wawasan baru tentang mekanisme internal yang mempengaruhi return saham, yang tidak sepenuhnya dijelaskan oleh penelitian - penelitian sebelumnya.
Determinan Audit Delay pada Perusahaan Makanan dan Minuman yang Terdaftar di BEI Tahun 2020-2022
Authors: Putri Emy Silvia , Pujiati Lilik
Published: September 2024

Latar Belakang: Meningkatnya kegiatan di Bursa Efek Indonesia menyebabkan peningkatan pendapatan dari saham-saham perusahaan publik sehingga diperlukan adanya audit laporan keuangan. Laporan keuangan tahunan dari perusahaan yang diperdagangkan di bursa harus diserahkan ke BAPEPAM-LK paling lambat tiga bulan setelah tahun fiskal berakhir. Menurut BEI, 68 perusahaan yang tercatat belum mengajukan laporan keuangan yang telah diaudit. Hal ini menunjukkan bahwa sejumlah besar bisnis masih mengalami keterlambatan dalam pelaporan keuangan yang telah diaudit. Tujuan: Tujuan dari penelitian ini adalah untuk menguji pengaruh ukuran perusahaan, ukuran KAP, profitabilitas dan solvabilitas terhadap audit delay pada perusahaan makanan dan minuman yang terdaftar di Bursa Efek Indonesia pada tahun 2020-2022. Metode Penelitian: Strategi yang digunakan dalam penelitian ini adalah purposive sampling, dengan ukuran sampel sebanyak dua puluh perusahaan. Informasi yang digunakan berasal dari laporan tahunan dan laporan keuangan perusahaan makanan dan minuman tahun 2020–2022 yang diperoleh dari situs web BEI (www.idx.co.id). Pengujian menggunakan analisis regresi berganda dengan dibantu menggunakan perangkat lunak SPSS versi 22.Hasil Penelitian: Hasil penelitian menunjukkan berdasarkan analisis yang telah dilakukan bahwa ukuran perusahaan tidak berpengaruh signifikan terhadap audit delay. Ukuran KAP berpengaruh negatif terhadap audit delay. Profitabilitas tidak berpengaruh terhadap audit delay. Solvabilitas berpengaruh positif terhadap audit delay.Keaslian/Kebaruan Penelitian: Penelitian ini memperluas studi sebelumnya dengan menambahkan satu variabel independen baru, yaitu Ukuran KAP, yang berfungsi sebagai variabel moderasi.
Clinical outcomes of reverse total shoulder arthroplasty (RTSA)
Authors: Mansotra Sahil , Hurkat Harshal , Oberoi Inderpreet Singh , Solanki Devendra Singh
Published: September 2024

Patients who have been diagnosed with glenohumeral arthritis due to inflammatory, degenerative and traumatic causes usually have a progressive course of disease and is very often not controlled with medication and conservative treatment. Most patients eventually end up with disabling pain, dysfunction and limited mobility requiring surgical intervention. Few treatment options are available when it comes to surgical intervention. These primarily include anatomical shoulder arthroplasty hemiarthroplasty and the more recent reverse total shoulder arthroplasty. Although approved by the United States Food and Drug Administration (FDA) in 2004, reverse total shoulder arthroplasty (RSA) has recently become popular worldwide as a treatment of choice for glenohumeral arthritis with promising outcomes and minimal complications.

Reverse Total Shoulder Arthroplasty (RTSA) is indicated for variety of shoulder conditions that involve a loss of rotator cuff function or massive tears, inflammatory pathology, gleno humeral arthritis complex proximal humerus trauma and other pathologies that cannot be effectively treated with traditional shoulder arthroplasty. RTSA can restore shoulder function and alleviate pain in these individuals. The goal of this study confirms previously reported improvements in pain, function scores, and range of motion, in patients treated with RTSA and to record clinical outcomes in Asian population.

To evaluate the clinical outcomes of patients undergoing reverse total shoulder arthroplasty (RTSA).

The present study was undertaken as a prospective, observational study among 30 Patients undergoing RTSA for various indications and attending the study hospital for medical care. The deltopectoral surgical approach was used on all participant placed in the beach chair position. Patients were followed up for 1 year postoperatively to asses outcome of RTSA.

Study showed participants were elderly(>60yrs) and female predominance. Mean body weight of participants was 57.37 ±4.25 kg, (95%CI 55.78-58.96 kg). Among 19 (63.3%) participants affected hand was right, 10 (33.3%) had left hand and 1 (3.3%) had bilateral limb affected. Study demonstrated significant improvements in all clinical outcomes measured using Constant Murley, ASES, SANE, SST scores and pain relief following shoulder surgery (p<0.05).

The study demonstrated significant improvements in functional outcomes and pain relief following shoulder surgery. The Constant Murley, ASES, SANE, and SST scores all showed consistent and substantial enhancement from preoperative to multiple postoperative time points, up to 1 year. Pain reduction was evident with a decrease in the VAS score.
Pueblos indígenas: brechas entre los sistemas de licenciamiento y fiscalización ambiental y los estándares internacionales
Authors: Indacochea Manuel , Acevedo Angela , Márquez Brander , León Diana
Published: September 2024

La implementación de sistemas nacionales de licenciamiento y fiscalización es fundamental para lograr el equilibrio entre el crecimiento económico y la preservación del entorno natural. Los países de la región han desarrollado marcos normativos y procedimientos de licenciamiento y fiscalización aplicables a proyectos de diversos sectores como la industria hasta la infraestructura, y consideran aspectos ambientales, ecológicos, sociales y culturales. En general, el diseño e implementación de proyectos de inversión con potenciales impactos adversos en territorios de pueblos indígenas o en los recursos naturales que utilizan, implica retos específicos para los sistemas nacionales de licenciamiento y fiscalización ambiental y social. Esta nota aborda algunos de los retos comunes que enfrentan los Gobiernos, pueblos indígenas y el sector privado en el marco de la implementación de procesos de licenciamiento y fiscalización ambiental y social. La nota aspira a contribuir diálogo sobre un conjunto de temas relevantes para la gestión oportuna y eficaz de asuntos sociales en proyectos con presencia de pueblos indígenas, a fin de fortalecer las buenas prácticas e identificar oportunidades de mejora.
Labor Market Effect of Granting Amnesty to Venezuelan Refugees and Migrants in the Dominican Republic
Authors: Loschmann Craig , Luzes Marta , Rivera Rivera Alejandra , van der Werf Cynthia
Published: September 2024

This paper examines the labor market impact of an amnesty program in the Dominican Republic, that grants Venezuelan refugees and migrants an ID card that allows them to work legally. To identify the effect, we compare the outcomes of those who received and did not receive ID cards before and after they were issued, leveraging the unexpected timing of their distribution. Our findings reveal negligible effects on the extensive margin, but indicate positive effects on the quality of employment, particularly in the likelihood of having a written contract and working in the formal sector. However, no discernible impact is observed on the likelihood of being overqualified for ones job or salary. The results suggest that the amnesty has helped Venezuelans integrate into the formal labor market, yet additional reforms such as title validation may be necessary to address the remaining barriers limiting full socio-economic inclusion.
Mirror, Mirror on the Wall: Which Jobs Will AI Replace After All?: A New Index of Occupational Exposure
Authors: Benites Miguel , Parrado Eric
Published: September 2024

This paper introduces the AI Generated Index of Occupational Exposure (GENOE), a novel measure quantifying the potential impact of artificial intelligence on occupations and their associated tasks. Our methodology employs synthetic AI surveys, leveraging large language models to conduct expert-like assessments. This approach allows for a more comprehensive evaluation of job replacement likelihood, minimizing human bias and reducing assumptions about the mechanisms through which AI innovations could replace job tasks and skills. The index not only considers task automation, but also contextual factors such as social and ethical considerations and regulatory constraints that may affect the likelihood of replacement. Our findings indicate that the average likelihood of job replacement is estimated at 0.28 in the next year, increasing to 0.38 and 0.44 over the next five and ten years, respectively. To validate our methodology, we successfully replicate other measures of occupational exposure that rely on human expert assessments, substituting these with AI-based evaluations. The GENOE index provides valuable insights for policymakers, employers, and workers, offering a data-driven foundation for strategic workforce planning and adaptation in the face of rapid technological change.
Mediação e suas escolas tradicionais
Authors: Salles Sergio , Faza Geovana
Published: September 2024

A fim de contribuir para o atual debate em torno da prática da mediação, objetiva-se, com este paper, sob as lentes das chamadas escolas de mediação e o impacto que elas possuem na atuação do facilitador, abordar o histórico de cada uma, sua fundamentação teórica e algumas técnicas que lhes caracterizam, assim como refletir sobre o papel do mediador de acordo com cada abordagem. As distinções entre os modelos serão feitas a partir de prismas diversos, análise bibliográfica e reflexões próprias, e as técnicas atinentes a cada uma foram retiradas de bibliografia dos próprios precursores das escolas, considerando, inclusive, atualizações realizadas após décadas de experiência prática, a exemplo da metodologia de Harvard e o programa desenvolvido por esta universidade.
EVALUATION OF THE EFFICACY OF CHLORINE-CONTAINING COMMERCIAL PRODUCTS FOR WATER DECONTAMINATION AGAINST COLIFORM BACTERIA
Authors: Sachkovska V. I. , Zinchenko O. Yu. , Zhukov B. S. , Kravets S. S.
Published: September 2024

Problem. Water is a key factor in the transmission of intestinal infections. During war conflicts, the risk of outbreaks of such infections increases due to infrastructure damage, the inaccessibility of central water supplies, and the impossibility of governmental institutions to control drinking water quality. In such situations, the need arises for household water treatment methods.
Aim. To evaluate the efficacy of seven commercial water treatment products available in the Ukrainian market against enteric bacteria and to compare their efficacy.
Methods. The activity of water purification products against the model microorganism Escherichia coli ATCC25922 was evaluated using serial dilution methods followed by viable count assessment.
The main results: High efficacy of the studied preparations against enteric bacteria was detected. In each test, a significant decrease in the initial titer of Escherichia coli from 1,2·10 6 CFU/cm 3 to ˂ 10 CFU/cm 3 was observed after the exposure time declared by the manufacturer.
Conclusions: The studied water treatment products from Ukrainian and foreign manufacturers were effective against enteric bacteria and met the WHO criteria for efficiency, regardless of the nature of the active compound (chlorine dioxide, sodium dichloroisocyanurate, or polyhexamethylene guanidine hydrochloride).
CIDADES, ECOLOGIA HUMANA E CRIMINOLOGIA AMBIENTAL: UMA RELEITURA DA OBRA DE ROBERT EZRA PARK
Authors: Guimarães Claudio Alberto Gabriel
Published: September 2024

Políticas públicas que indiquem caminhos para enfrentar os mais diversos tipos de violência oriundos das cada vez mais frequentes vulnerabilidades a que estão sujeitos os habitantes das cidades são uma temática imprescindível no debate acadêmico contemporâneo, razão pela qual os fundamentos teóricos que apontem aportes epistemológicos, metodológicos e criminológicos para alcance de tal intuito devem ser apresentados, debatidos e atualizados, ou seja, pretende-se aprofundar a discussão sobre a consecução de políticas públicas efetivas, eficazes e eficientes que se constituam óbice a um contexto de crescente violência, sobretudo a criminal. Essa é a razão pela qual é primordial revisitar a obra de Robert Ezra Park e, consequentemente, o estudo da produção da escola sociológica de Chicago no âmbito da ecologia humana, trabalho que apresenta pelo menos 100 anos de contribuição para a produção científica e, portanto, os fundamentos analíticos nas mais diferenciadas áreas das ciências humanas. Foi adotado o método indutivo para construção do argumento, com procedimento descritivo baseado na técnica de pesquisa exclusivamente bibliográfica.
Optimizacija uporabe CI/CD cevovodov, DevOps pristopa in oblaka
Authors: Škarabot Mihael
Published: September 2024

Članek obravnava ključne koncepte neprekinjene integracije, izdaje in nameščanja (CI/CD+CD) kot temeljne elemente za razvoj sodobne programske opreme za nudenje SaaS (ang. Software-as-a-Service). Poudarja pomen DevOps metod in naprednih arhitekturnih pristopov, kot so mikro storitve in modularni monoliti, ki omogočajo večjo skalabilnost, dostopnost, zanesljivost in učinkovitost rešitev. Različni tipi CI/CD cevovodov, prilagojeni specifičnim potrebam, optimizirajo proces razvoja in zagotavljanja programske opreme. Članek izpostavlja prednosti uporabe javnih oblačnih storitev oziroma hiperoblačnih storitev, ki zaradi svoje zanesljivosti, elastične skalabilnosti in transparentnih stroškov predstavljajo idealno infrastrukturo za globalne SaaS rešitve. Prav tako poudarja pomembnost avtomatizacije testiranja in posodobitev skozi CI/CD cevovode, kar prispeva k redkejšim napakam in večji učinkovitosti. Poleg tega članek obravnava strategije za učinkovito upravljanje stroškov v hiperoblaku, vključno z rezervacijami in varčevalnimi načrti, ter različne načine elastičnega skaliranja, kot so vertikalno in horizontalno skaliranje zabojnikov in strežnikov. Poudarja tudi pomen uporabe PaaS storitev za zmanjšanje kompleksnosti in izboljšanje zanesljivosti. Zaključuje z ugotovitvijo, da učinkovita strategija uporabe CI/CD cevovodov in javnih oblakov omogoča podjetjem hitrejše prilagajanje tržnim potrebam ter zagotavljanje visokokakovostnih storitev na globalnem trgu, kar je ključno za uspeh sodobnih SaaS rešitev.
Liberties of the genome: insertions of mitochondrial DNA fragments into nuclear genome
Authors: Golubenko M. V. , Puzyrev V. P.
Published: September 2024

The transition of detached fragments of mitochondrial DNA into the nucleus and their integration into chromosomal DNA is a special kind of genetic variability that highlights the relation between the two genomes and their interaction in a eukaryotic cell. The human genome contains several hundreds of insertions of mtDNA fragments (NUMTS). This paper presents an overview of the current state of research in this area. To date, evidence has been obtained that the occurrence of new mtDNA insertions in the nuclear genome is a seldom but not exceptionally rare event. The integration of new mtDNA fragments into the nuclear genome occurs during double-strand DNA break repair through the non-homologous end joining mechanism. Along with evolutionarily stable “genetic fossils” that were integrated into the nuclear genome millions of years ago and are shared by many species, there are NUMTS that could be species-specific, polymorphic in a species, or “private”. Partial copies of mitochondrial DNA in the human nuclear genome can interfere with mtDNA during experimental studies of the mitochondrial genome, such as genotyping, heteroplasmy assessment, mtDNA methylation analysis, and mtDNA copy number estimation. In some cases, the insertion of multiple copies of the complete mitochondrial genome sequence may mimic paternal inheritance of mtDNA. The functional signiﬁcance of NUMTS is poorly understood. For instance, they may be a source of variability for expression and splicing modulation. The role of NUMTS as a cause of hereditary diseases is negligible, since only a few cases of diseases caused by NUMTS have been described so far. In addition, NUMTS can serve as markers for evolutionary genetic studies. Of particular interest is the meaning of NUMTS in eukaryotic genome evolution. The constant flow of functionally inactive DNA sequences from mitochondria into the nucleus and its significance could be studied in view of the modern concepts of evolutionary theory suggesting non-adaptive complexity and the key role of stochastic processes in the formation of genomic structure.
Generation and analysis of mouse embryonic stem cells with knockout of the Mcph1 (microcephalin) gene
Authors: Yunusova A. M. , Smirnov A. V. , Shnaider T. A. , Pristyazhnuk I. E. , Korableva S. Y. , Battulin N. R.
Published: September 2024

Chromatin is not randomly distributed within the nucleus, but organized in a three-dimensional structure that plays a critical role in genome functions. Сohesin and condensins are conserved multi-subunit protein complexes that participate in mammalian genome organization by extruding chromatin loops. The fine temporal regulation of these complexes is facilitated by a number of other proteins, one of which is microcephalin (Mcph1). Mcph1 prevents condensin II from associating with chromatin through interphase. Loss of Mcph1 induces chromosome hypercondensation; it is not clear to what extent this reorganization aﬀects gene expression. In this study, we generated several mouse embryonic stem cell (mESC) lines with knockout of the Mcph1 gene and analyzed their gene expression profile. Gene Ontology analyses of diﬀerentially expressed genes (DEGs) after Mcph1 knockout revealed gene categories related to general metabolism and olfactory receptor function but not to cell cycle control previously described for Mcph1. We did not find a correlation between the DEGs and their frequency of lamina association. Thus, this evidence questions the hypothesis that Mcph1 knockout-mediated chromatin reorganization governs gene expression in mESCs. Among the negative eﬀects of Mcph1 knockout, we observed numerous chromosomal aberrations, including micronucleus formation and chromosome fusion. This confirms the role of Mcph1 in maintaining genome integrity described previously. In our opinion, dysfunction of Mcph1 may be a kind of “Rosetta stone” for deciphering the function of condensin II in the interphase nucleus. Thus, the cell lines with knocked-out Mcph1 can be used to further study the influence of chromatin structural proteins on gene expression.
Advancing The Blue Economy Through Sustainable Mariculture: The Prospect of Pearl Oyster and Sponge Cultivation in St. Vincent and The Grenadines
Authors: Providence Kevin Antonio
Published: September 2024

The Blue Economy is estimated to be worth over USD 2.5 trillion annually, or almost 3% of the world’s GDP in 2020. St. Vincent and the Grenadines (SVG) in the Caribbean region has a vast and rich marine space that is underutilized. This study aimed to explore the feasibility of pearl oyster and sponge farming to advance the country’s Blue Economy through sustainable mariculture. A qualitative approach was applied, using a literature review and thematic analysis of data gathered through semi—structured interviews and focus group discussions with relevant stakeholders. Among several species evaluated, the result of the study revealed that the Pinctada imbricata oyster species and Hippospongia lachne sponge species are the most suitable for cultivation in SVG coastal waters, specifically in the Ashton Lagoon of Union Island. The study concluded that while there is strong support from the stakeholders in SVG for pearl oyster and sponge farming in the country, challenges still exist. Nevertheless, it has been found that by learning and adopting the strategies of the leading Small Island Developing States (SIDS) in the Blue Economy development combined with the critical enablers such as meticulous planning, stakeholder collaboration, establishment of legal frameworks, and ecosystem—based management, pearl oyster and sponge mariculture in the identified location in SVG could potentially aid the expansion of the country’s Blue Economy.
Financial Socialization in Christian Premarital Counseling With Emerging Adults
Authors: Irby Courtney Ann
Published: September 2024

Research on financial socialization among emerging adults has retrospectively examined what they have learned from their parents growing up. Building on this work, this article explores how these lessons are not static but can become reexamined through interpersonal relationships. It reports on findings from a qualitative study of four Christian premarital programs (two evangelical Protestants and two Catholics) and 70 interviews with participating couples and leaders of these and other programs. By contributing observational data on how the methods of socialization operate, it analyzes both explicit lessons on money and implicit models for how premarital counselors construct normative frameworks on how to interrogate emerging adult’s past and plan for their financial future. Specifically, it documents how financial advice was often relegated to supplemental materials due to leaders’ lack of confidence on money matters but also how the broader programming provided young couples with opportunities to identify and reflect on their family financial socialization. Implications for professionals interested in improving premarital and financial counseling are discussed.
Aplikasi Edible Coating dari Lidah Buaya dan Karagenan untuk Memperpanjang Umur Simpan Buah Tomat (Lycopersicum esculentum Mill.)
Authors: Siregar Tagor M. , Elysia Vanessa
Published: September 2024

Tomato fruit (Lycopersicum esculentum Mill.) is a food material with a high water content so it is easy to decompose. This shows that efforts to prevent reduction in food quality can be developed in the form of edible coating from aloe vera gel with concentration of 0%, 30%, 40%, and 50%, and carrageenan with concentration of 1%, 2%, and 3%. The aims of this research were to determine the concentration of aloe vera gel and carrageenan based on the characteristics of edible film and to determine the effect of edible coating and storage time on the quality of tomato. The first stage of this research is making edible film based on analysis parameters such as water vapor transmission rate (WVTR), tensile strength, elongation, and thickness. The selected concentration used in the second stage of this research for making edible coating was 40% of aloe vera gel and 3% of carrageenan. The selected concentration has a WVTR value of 1.758±0.20 g.mm/m2.hour, tensile strength value of 0.248±0.00 N/cm2, elongation value of 25.164±0.10%, and thickness value of 0.074±0.00 mm. Tomato samples coated with edible coating and control tomatoes is stored for 9 days at room temperature (24-25oC and 65–70% relative humidity aproximately) and samples are analyzed every 3 days based on analysis parameters such as vitamin C content, weight loss, hardness level, pH, ohue, lycopene content, and total dissolved solids value. The application of edible coating and storage time can inhibit the decrease of vitamin C levels, lycopene levels, weight loss, hardness, also can inhibit the increase in pH value and total dissolved solids value. Meanwhile, the change in color (hue) of tomato is not affect by the use of edible coating.
Keywords: aloe vera, carrageenan, edible coating, storage time, tomato
Karakteristik Fisik dan Kandungan Gizi Tepung Pisang Lokal Lampung dengan Metode Perebusan
Authors: Nirmagustina Dwi Eva , Hidayat Beni , Zukryandry Zukryandry
Published: September 2024

Lampung Province is the third largest banana producer in Indonesia with various types of bananas. It affects the differences of physical characteristics and nutritional content. Besides the type of banana, the processing can also affect the differences in its characteristics. The objective of the research was to determine the physical characteristics and nutritional content of banana flour from 8 types of bananas grown in Lampung Province as a source of local flour with the boiling method. The study was designed in a completely randomized block design with 3 replications. The results showed that the type of banana affected the physical characteristics and nutritional content of banana flour. Nangka bananas had the highest yield (42.06%) and muli bananas had the lowest yield (24.45%). Ambon kuning and tanduk had the highest degree of whiteness (44.60 and 41.20) and this is influenced by the color of the black pith. Muli banana flour was the highest protein content (3.54%). Fat content in nangka banana flour is the highest (1.09%). Ash of muli banana and ambon banana flour were the highest content (2.99% and 2.68%). Crude fiber of muli banana flour and kepok manado banana flour were the highest content (3.17% and 3.21%). The starch, amylose, and ratio of amylose/amylopectin of kepok manado and tanduk banana flour were the same and the highest content (respectively ranging between 74.53–76.29%, 26.18–28.92%, and 0.35–0.41). Vitamin C and total acid in local banana flour ranged from 0.51–2.34 mg/100 g and 0.62–2.25 respectively, muli bananas had the highest content of vitamin C and total acid with values of 2.34 mg/100 g and 2.25%. The boiling process can be applied as one of pre-treatment before making local Lampung banana flour.
Keywords: banana flour, boiling method, local banana, nutritional
Analisis Keberlanjutan Agroindustri Ikan Lemuru (Sardinella sp.) (Studi Kasus di Kecamatan Puger, Kabupaten Jember)
Authors: Purnomo Bambang Herry , Wibowo Yuli , Suryaningrat Ida Bagus , Novijanto Noer , Suryadharma Bertung , Fahmi Achmadi Anwarul Anwarul
Published: September 2024

The capture fisheries agroindustry faces serious business sustainability threats in the future, both from economic, resource, environmental, technological and social aspects. Lemuru fish (Sardinella sp.) based agroindustry in Puger District, Jember Regency also faces a similar threat. This research aims to analyze the sustainability of the lemuru fish processing business and provide recommendations for improving its sustainability. The analytical method used is Rapfish (Rapid Appraisal for Fisheries), while the recommendations are prepared descriptively. The results of the analysis show that the Lemuru agro-industry sustainability index is in the less sustainable category with a score of 42.61. The environmental dimension has the lowest index value in the bad category, followed by the social and technological dimensions which are categorized as less sustainable, while the resource and economic dimensions are categorized as quite sustainable. Efforts to improve the environmental dimension can be made by organizing mentoring programs to develop environmental management efforts. For the social and technological dimensions, technological guidance, management and horizontal diversification of processed lemuru waste can be carried out into various innovative products.
Keywords: capture fisheries, lemuru, sustainability analysis, Rapfish
Strongyloides stercoralis in Low-Income Immigrants from Portuguese Speaking African Countries in Lisbon, Portugal
Authors: Carinton Diamarize , Teodósio Rosa , Belo Silvana , L. Maurício Isabel
Published: September 2024

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Recovery como Objetivo Terapêutico na Esquizofrenia: O Dever de Oferecer Mais
Authors: Amorim Maria João , Perestrelo Passos Patrícia
Published: September 2024

N/a.
Sensitivity Analysis of C. auris, S. cerevisiae, and C. cladosporioides by Irradiation with Far-UVC, UVC, and UVB
Authors: Gierke Anna-Maria , Hessling Martin
Published: September 2024

Background: The World Health Organization has published a list of pathogenic fungi with prioritizing groups and calls for research and development of antifungal measures, with Candida auris belonging to the group with high priority.
Methods: The photosensitivity towards short wavelength ultraviolet irradiation (Far-UVC, UVC, and UVB) was investigated and compared to other yeasts (Saccharomyces cerevisiae) and a mold (Cladosporium cladosporioides). The observed 1-log reduction doses were compared to literature values of other representatives of the genus Candida, but also with S. cerevisiae, Aspergillus niger, and A. fumigatus.
Results: For the determined 1-log reduction doses, an increase with higher wavelengths was observed. A 1-log reduction dose of 4.3 mJ/cm2 was determined for C. auris when irradiated at 222 nm, a dose of 6.1 mJ/cm2 at 254 nm and a 1-log reduction dose of 51.3 mJ/cm2 was required when irradiated with UVB.
Conclusions: It was observed that S. cerevisiae is a possible surrogate for C. auris for irradiation with Far-UVC and UVB due to close 1-log reduction doses. No surrogate suitability was verified for C. cladosporioides in relation to A. niger and A. fumigatus for irradiation with a wavelength of 254 nm and for A. niger at 222 nm.
Immunohistochemical Investigation of P16 Expression in Curettage Biopsies
Authors: Yurtkal Aslıhan , Canday Müjde , Beşeren Hatice
Published: September 2024

Our study aims to investigate the immunohistochemical expression of the P16 molecule, which is involved in the cell cycle and plays a role in developing endometrial cancer in normal epithelium, endometrial polyp, and precursor lesions. A total of 68 patients underwent endometrial sampling for various reasons at the Department of Obstetrics and Gynecology, Faculty of Medicine, Kafkas University, between 2020 and 2021 were included in the study. The selected cases were categorized into four groups: proliferative endometrium, endometrial hyperplasia without atypia, atypical hyperplasia / endometrioid intraepithelial neoplasia (AH / EIN) and endometrial polyp. There were no cases with a diagnosis of endometrial tumors in our study. All patients’ pathology samples were re-evaluated, and P16 immunohistochemistry was applied to tissue samples. Among patients diagnosed with atypical endometrial hyperplasia, 72.7% exhibited moderate P16 protein expression, 18.2% had low expression, and 9.1% had high protein expression. The number of patients diagnosed with AH / EIN had a very low frequency in the study population. Among patients diagnosed with endometrial polyps, 50.0% showed moderate P16 protein expression, 20.0% exhibited low protein expression, and 30.0% had high protein expression. High P16 expression has been reported to be significantly associated with endometrial cancer in the literature. P16 expression is significant in precancerous lesions and stages of cancer development. Larger-scale studies with more cases are needed in this regard.