bluuebunny/crossref_metadata_2025 huggingface dataset:
TikTok and Researcher Positionality: Considering the Methodological and Ethical Implications of an Experimental Digital Ethnography
Authors: Zhao Yang
Published: January 2024

In this article I examine the opportunities and challenges arising from an experimental digital ethnography I conducted as a digital content creator in response to social restrictions during COVID-19. To explore the perceptions and performances of masculinity among young Uzbek men in Uzbekistan, I created 50 TikTok videos between 2021 and 2022. These videos received more than 300,000 likes in total, not only significantly broadening the reach of my research recruitment but also serving as a substantial source of ethnographic data during the pandemic. Throughout the creation of these digital videos, I assumed a dual role as an agent in the research and an object of observation. This dual role underscores the agency of both researchers and the researched in navigating the digital platform, which allows for the challenging of conventional research gazes and relationships. This digital approach also unveils the complex spatial dynamics that underlie interactions in both online and offline realms, shedding light on how digital platforms can both enhance and constrain research efforts. Moreover, this article delves into the ethical implications of this experimental digital ethnography, which revolve around potential physical and mental risks to researchers, challenges related to the re-definition of research participation, and issues pertaining to obtaining informed consent. The findings provide insights and make contributions to problematising the conceptualisation of digital spaces, online communities/publics and digital ethnography. I conclude by offering insights for researchers who face restrictions in field access or are interested in studying youth culture on social media platforms, particularly in the role of a content creator, an area that has been relatively underexplored in previous research.
Structure and properties study of magnesium oxide porous ceramics prepared by Organic Foam Template Method
Authors: Sun Minghui , Wang Shaolong , Zhang Yani , Wang Tun , Li Shengchun , Zhang Lei , Lei Fang , Fan Lingcong , Shi Ying , Xie Jianjun
Published: January 2024

Magnesium oxide (MgO) porous ceramics with high porosity, compressive strength and low thermal conductivity were prepared by Organic Foam Template Method. The effects of the sintering temperature, polycarboxylic acid (PCE) dispersant and pore size of organic foam template on the properties of MgO porous ceramics were investigated. The experiment results showed that with the increase of sintering temperature, the MgO porous ceramic shrinkage, skeleton density and compressive strength increased. PCE could increase the fluidity of slurry and make the framework clearer, as well as reduce the cracks formed in the process of drying effectively. When the content of PCE was 0.5 wt-%, the porosity, compressive strength and thermal conductivity of MgO porous ceramics were 88.5%, 1.6 MPa and 0.045 W/(m·K), respectively. In addition, as the pore size of the organic foam template decreased, the porosity decreased, and the resistance increased and the thermal conductivity increased.
Curriculum effectiveness for secondary-aged students with severe intellectual disabilities or profound and multiple learning difficulties in Australia: Teacher perspectives
Authors: Rendoth Tess , Duncan Jill , Foggett Judith , Colyvas Kim
Published: January 2024

The active inclusion of students within education systems relies on a curriculum that caters to all. This article presents partial findings from Australian mixed methods research examining 46 teacher perspectives on the curriculum and its ability to support their practice in supporting students aged 12-19 years with severe intellectual disability or profound and multiple learning difficulties who attend specialist school settings. Results reveal that Australian teachers see the current curriculum as insufficient in its design and content and unable to cater to their students educational and social capacities or needs. Strengths essential to the reform process are highlighted, emergent challenges discussed and recommendations for future action are presented.
Physical education orientation and activity among youth ice hockey coaches
Authors: Raudasoja Iina , Vasankari Tommi , Simula Mikko , Villberg Jari , Vähä-Ypyä Henri , Kokko Sami
Published: January 2024

Sixty-two percent of Finnish children and adolescents aged 9–15 participate in sports club activities, yet only one-third of sports participants reach the recommended 60 min of daily moderate-to-vigorous physical activity. The inadequate physical activity of participants underlines the need for physical education among those participating in sports club activities. Sports coaches have significant potential in promoting participants’ weekly overall physical activity beyond sports training, given their role of authority. However, coaches in Finland mainly work on a voluntary basis, with varying knowledge and capabilities. The aim of the study was to investigate the physical education orientation and activity of sports coaches, and the associations of these with the moderate-to-vigorous physical activity of young sports participants. Questionnaire data from coaches ( n = 26) and young sports participants aged 6–18 ( n = 201), and accelerometer data from young sports participants ( n = 293) were included in the analysis. The results indicated that even though sports coaches recognized the importance of physical education, they have not fully embraced their role as physical educators and promoters of weekly overall physical activity among young sports participants. The role of families was considered most significant in physical education. Coaches perceived their possibilities to influence the physical activity behavior of the participants as limited. Coaching education and experience had a positive relationship with the coaches’ activity in implementing physical education, highlighting the importance of coaching education. The findings suggest that there is a need to increase collaboration with coaches and families to ensure an adequate amount of weekly overall physical activity of children and adolescents.
Rethinking the Healthcare Facilities: The Role of the Buffer Space
Authors: Gola Marco , Johnson Alexander Achille , La Milia Daniele Ignazio , Cadeddu Chiara , Bardini Francesco , Bianconi Barbara , Bisceglia Raffaella , Pumpo Marcello Di , Genovese Cristina , Grieco Albino , Piras Giuseppe , Guerra Rocco , Damiani Gianfranco , Favaretti Carlo , Montagna Maria Teresa , Capolongo Stefano , Ricciardi Walter
Published: January 2024

Objectives:

A working group conducted a survey on the use of the principle of buffer space (BS), which in case of emergencies, could benefit healthcare settings. The aim of the preliminary investigation is to define new research lines in hospitals’ functional design.

Background:

The global experience of the COVID-19 pandemic highlighted challenges faced by hospitals when responding promptly to emergencies, including spatial reorganization and suspension of ordinary medical activities for ensuring adequate management of the emergency surge of patients.

Methods:

The group designed questionnaires to be administered to healthcare staff and healthcare designers aimed at understanding varied conceptions and features of BSs. Content across the two surveys overlapped significantly, allowing for direct comparisons of responses, while also including tailored questions in relation to the respective experience and skills of the two groups of respondents.

Results:

102 healthcare professionals and 56 designers took part to the survey. Analysis of the responses permitted for initial recommendations regarding BS typology including (a) proximity to the emergency department (ED), intensive care units (ICUs), and inpatient wards (IWs); (b) location within hospitals but separate from other medical areas; (c) need for independent access; (d) organizational and spatial features similar to ED, ICUs, and IWs; (e) existing as a fully flexible operational space; and (f) BS bed capacity to be approximately 12% of ED beds.

Conclusions:

Although the analysis is related to the Italian context, the expansion of this preliminary research to alternate healthcare facilities and geographic areas is necessary for reaching a wide consensus by different professionals on this field. It serves as a starting point for future investigations regarding the implementation of BS in hospital settings.
Willingness to take human papillomavirus vaccine and its associated factors among school adolescent girls: A case of school-based dose one human papillomavirus vaccine campaign in Dire Dawa, Ethiopia
Authors: Kassie Nigus , Sema Alekaw , Amsalu Bezabih , Sintayehu Yitagesu , Abie Alemwork , Mengist Betlhem , Yibabie Shegaye , Talie Enyew
Published: January 2024

Objective:

This study aimed to evaluate the willingness of human papillomavirus vaccine and its associated factors in Dire Dawa in 2022. Ethiopia is undergoing human papillomavirus vaccine implementation in the country with the support of the Global Alliance for Vaccines and Immunization. However, there is a scarcity of data on problems encountered and the receiver’s acceptance of the vaccine.

Methods:

An institution-based cross-sectional study was conducted among 634 female adolescents from 1 April to 1 May 2022. A simple random sampling technique was used to select study participants. Pretested structured self-administered questionnaires were used to collect data. Data were entered into EPI-data 3.1 and exported to Statistical Package for the Social Sciences version 23.00 software for analysis. Descriptive analysis was conducted to summarize data with text, tables, and figures. A binary logistic regression model was fitted to identify factors associated with the willingness of the human papillomavirus vaccine. Adjusted odds ratios with 95% confidence intervals and p-values less than 0.05 were used to determine the association between independent and dependent variables. Hosmer and Lemeshow test was used to determine model fitness.

Results:

The magnitude of human papillomavirus vaccine willingness among adolescent girls was 56% with 95% CI (52.1%–59.9%). Discussion on sexual and reproductive health issues (AOR = 7.67, 95% CI: 3.82, 15.42), adolescent girls who were received permission from their family to take human papillomavirus vaccine (AOR = 4.49, 95% CI: 2.40, 8.43), adolescent girls who had good knowledge on human papillomavirus vaccine (AOR = 5.78, 95% CI: 3.18, 10.15), and adolescent girls who had favorable attitude toward human papillomavirus vaccine (AOR = 3.06, 95% CI: 1.73, 5.43) were positively associated with willingness to take human papillomavirus vaccine.

Conclusion:

Knowledge about cervical cancer and the human papillomavirus vaccine as well as willingness to take the human papillomavirus vaccine was low. Factors such as maternal education, adolescents’ discussion on sexual and reproductive health issues, parent approval of human papillomavirus vaccine, and knowledge and attitude toward human papillomavirus vaccine were significantly associated with willingness to take the human papillomavirus vaccine. Therefore, public health education regarding cervical cancer and human papillomavirus vaccination is still needed and has to be targeted at not only respondents but also their families.
Using MI-LASSO to study populist radical right voting in times of pandemic
Authors: Chan Ka Ming , Stephenson Laura B
Published: January 2024

As immigration issues waned in salience during the COVID-19 pandemic, populist radical right (PRR) parties repositioned themselves by politicizing various pandemic policies. In light of this changing political landscape, scholars have analyzed what factors are associated with PRR voting. Yet, most studies focus on small sets of covariates that could easily ignore other key determinants. To address this limitation, we use MI-LASSO logistic regression, which is a more inductive data-driven approach that can incorporate a huge number of covariates. Our research analyzes the key determinants of voting for the People’s Party of Canada—a PRR party that rose rapidly during the pandemic. Using the 2021 Canadian Election Study dataset ( N = 14,841), we confirm that PRR voters in the pandemic were both protest and policy-oriented voters. They were protest voters since anti-establishment attitudes consistently correlate with their vote choice. On the other hand, PRR voters’ policy concern was about pandemic policies rather than immigration, as nativist attitudes never emerge as key determinants. Additionally, we uncover that the ideological placement of the mainstream right party and the defense of hate speech are strong correlates, while conventional variables like sociodemographics are not. These findings enrich our understanding of PRR voting during the pandemic.
Effect of diazoxide on a cat with insulinoma
Authors: Sato Shiori , Hori Koji , Tanabe Gaku , Maeda Shingo , Momoi Yasuyuki , Yonezawa Tomohiro
Published: January 2024

Case summary

The patient was a castrated male American Shorthair cat, approximately 14 years old, weighing 3.4 kg. The patient had chronic kidney disease (CKD) (International Renal Interest Society stages 3–4) as an underlying disease. The cat was examined at a hospital for intermittent lethargy and seizures. Hypoglycaemia was repeatedly observed, and the insulin level was 1.78 ng/ml (reference interval 0.27–0.69) when the blood glucose was 49 mg/dl. Although the cat was tentatively diagnosed with insulinoma, surgery was not recommended because of the severe CKD. Although frequent feeding and prednisolone treatment were initially attempted, blood glucose decreased to 24–42 mg/dl. Diazoxide was additionally prescribed at a dose of 5.2 mg/kg q12h. The cat’s clinical signs improved, and the blood glucose was in the range of 75–103 mg/dl during the first 2 months. It was maintained at >50 mg/dl until the patient died of renal failure 161 days after the start of diazoxide treatment. With regard to adverse events, vomiting once every 2–3 days without weight loss and non-regenerative anaemia were observed, which might have been at least partially caused by diazoxide treatment. An insulinoma was definitively diagnosed via pathological autopsy.

Relevance and novel information

This is the first reported case of long-term treatment with diazoxide in a cat with insulinoma. Since it was effective in situations where conventional therapies were unsuccessful, diazoxide could be useful as a new therapeutic option for cats with insulinoma. Since adverse events, such as progression of vomiting frequency and non-regenerative anaemia, were observed, careful monitoring was required during administration.
Early Wound and Sternal Management Following Pediatric Cardiac Surgery
Authors: Gal Dana B. , Cleveland John D. , Kipps Alaina K.
Published: January 2024

Early postoperative wound management following congenital heart surgery remains an area without equipoise. Precautionary restrictions can impact quality of life, development, and delay access to other needed care. The influence of different practices on wound healing and complications is unknown. We surveyed Pediatric Acute Care Cardiology Collaborative member centers regarding postoperative wound closure, wound vacuum-assisted closure (VAC) use, sternal precautions, and restrictions in the early postoperative period. We analyzed responses using descriptive statistics. Responses were submitted by 35/46 (76%) centers. Most centers perform primary skin closure with subcutaneous sutures. Wound covers are removed after 48 h at 43% (15/35) of centers and after ≥72 h at 34% (12/35) of centers. For delayed sternal closure, 16 centers close skin with interrupted, externalized sutures, 5 utilize wound VAC-assisted closure, and 12 use variable practices. Generally, 33 centers use wound VACs for wound care. Patient selection for VAC use and length of therapy varies. We found great variability in duration of sternal precautions and in activity, bathing, and submersion restrictions. Finally, 29 centers require a waiting period between cardiothoracic surgery and other surgeries such as tracheostomy or gastrostomy tube placement. Postoperative wound and sternal management lack consistency across North American pediatric heart institutes. Some restrictive practices may prolong length of stay and/or negatively impact quality of life and neurodevelopment. Practices may also impact wound infection rates. Research linking practices with clinical outcomes is needed to better define standards of care and reduce potential negative consequences of overly conservative or aggressive practices.
A Prospective Multicenter ≥2 Years Clinical Study of the Active Apex Correction (APC) Technique in Early Onset Scoliosis (EOS) Patients
Authors: Hammad Ahmad M. , Emar Mohammad , Shahin Farah , Aljuba Ayat , Hasani Asala , Awad Mohammad , Abdelnabi Siraj , Gayaswal Daksh , Armouti Mohammad , Shekhar Shubhankar , Garg Bhavuk , Ahmad Alaaeldin A.
Published: January 2024

Study Design

prospective multicenter study.

Objectives

Active apex correction (APC) is posterior tethering technique for correction of early onset scoliosis (EOS) via reverse modulation at the apex. Active apex correction has been increasingly used worldwide. This study aimed to assess short-term outcomes of multicenter study with ≥2 years of APC on spine length, curve correction, complications, unplanned surgeries, and proposed low crankshaft phenomena incidence.

Methods

Prospective multicenter study including 24 EOS patients treated by APC; involves inserting and compressing pedicle screws on convex side of apex proximal and distal to most wedged vertebra allowing apex modulation according to Hueter-Volkmann law. Excluded patients with <2 years follow-up whom APC was not primary surgery.

Results

Mean age 85.97 ± 32.43 months, 71% congenital scoliosis, mean follow-up 35.54 ± 12.36 months. At final follow-up, statistically significant improvement in Cobbs angle (∆ = 23.96%, P < .0001), spinal length T1-T12 (∆ = 12.83%, P < .0001), T1-L5 (∆ = 13.41%, P < .0001) but not in apical vertebral translation (AVT) albeit clinical improvement (∆ = 7.9%, P = .36) compared to preoperative measurements. Comparing immediate postoperative measurements to >2 years follow-up, statistically significant improvement in spinal length T1-T12 (∆ = 6.03%, P = .0002) and T1-L5 (∆ = 6.26%, P < .0001) but not in Cobbs angle (∆ = 4.93%, P = .3) or AVT (∆ = 14.77%, P = .25). 9 complications requiring 3 unplanned surgeries recorded in all patients including 2 broken rods, 2 adding-on and 4 screw dislodgement.

Conclusion

Active apex correction is a novel technique that has been incorporated in several countries as treatment modality for EOS. Short-term outcomes are promising in terms of clinical improvement, complication rates and decreased need for multiple operations or unplanned surgeries.
Measuring the Impact of Surgical and Non-surgical Facial Cosmetic Interventions Using FACE-Q Aesthetic Module Scales: A Systematic Review and Meta-Analysis
Authors: Gallo Lucas , Kim Patrick , Churchill Isabella F. , Gallo Matteo , Yuan Morgan , Voineskos Sophocles H. , Thoma Achilles , Pusic Andrea L. , Klassen Anne F. , Cano Stefan J.
Published: January 2024

Background: The FACE-Q Aesthetic module measures patient-important outcomes following surgical and non-surgical facial cosmetic procedures. Objective: The primary aim of this systematic review was to summarize the pre- to post-intervention mean differences of facial aesthetic interventions that evaluate outcomes using the FACE-Q Face Overall, Psychological, and Social scales. Methods: Ovid Medline, Embase, Cochrane, and Web of Science databases were searched on December 20, 2022 with the assistance of a health-research librarian (CRD42023404238). Studies that examined any surgical or non-surgical facial aesthetic intervention in adult patients and used FACE-Q Aesthetics Face Overall, Psychological, and/or Social scales to measure participants before and after treatment were included for analysis. Results: Of 914 potential articles screened, 35 studies met the inclusion criteria. Most studies evaluated surgical (n = 22, 62.9%) versus non-surgical facial cosmetic interventions (n = 13, 37.1%). Rhinoplasty [37.0 points, 95% CI 24.7-49.3, P < 0.01] demonstrated the largest weighted increase in Face Overall scores, whereas the largest increase in Psychological [67.1 points, 95% CI 62.9–71.3, P < 0.01] and Social [63.9 points, 95% CI 53.2–74.6, P < 0.01] scores was demonstrated by a single study evaluating surgical forehead lifts, respectively. Conclusions: This meta-analysis leverages FACE-Q Aesthetic module scoring to present the expected mean differences in Face Overall, Psychological, and Social scale scores for various surgical and non-surgical facial cosmetic interventions. The findings from this review may be used to indirectly compare interventions and contribute to sample size calculations when planning future studies.
Assessment of Research Ethics Knowledge of Pediatricians
Authors: Salayev Kamran , Aslanova Ulviyya , Guliyeva Narmin , Geraybeyli Geray , Munir Kerim
Published: January 2024

Objectives. To assess knowledge of ethics knowledge among child healthcare professionals. Methods. We translated the Test of Residents’ Ethics Knowledge for Pediatrics (TREK-P) in Azeri and administered it to: (i) third-year medical students (n = 21), (ii) pediatrics residents (n = 24), (iii) practicing pediatricians (n = 21), and (iv) fellows (n = 9) who participated in a Fogarty International Center/National Institute of Health (Fogarty/NIH) R25 research ethics education program. The difference in the TREK-P score between the groups and the correlation between the TREK-P score and other factors were evaluated. Results. The fellows scored significantly higher than the other groups ( P = .006). There was no significant difference between the other 3 groups. Within a joined group of pediatricians and fellows, previous training on ethics was the only factor that correlated with the higher TREK-P scores ( P < .05). Conclusions. The higher scores in fellows support the effectiveness of postgraduate Fogarty/NIH training programs in research ethics.
A Dental Extraction Revealing a Multisystem Burkitt’s Lymphoma: A Case Report
Authors: Chait Fatima , Bahlouli Nourrelhouda , Laasri Khadija , Sfar Kaouthar , Lamalmi Najat , Allali Nazik , El Haddad Siham , Chat Latifa
Published: January 2024

Burkitt’s lymphoma is rare but highly aggressive and very fast-growing B-cell non-Hodgkin’s lymphoma (NHL). It can affect any organ such as the central nervous system, jaw, intestines, kidneys, ovaries, and other organs. It results from the malignant evolution and proliferation of B-type lymphoid cells. The diagnosis is based on a biopsy of a tumor mass or bone marrow aspiration revealing the presence of tumor cells. We report the case of a 7 year old child who was referred for a gingival swelling evolving since 1 month following a dental extraction. Imaging and anatomopathological examination after biopsy concludes to a multi systemic Burkitt’s lymphoma. A chemotherapy was immediately started with spectacular complete remission.
Myths About Breastfeeding as Voiced by Lactating Nurses and Midwives: A Qualitative Study at the Greater Accra Region of Ghana
Authors: Acheampong Angela Kwartemaa , Abukari Alhassan Sibdow
Published: January 2024

Introduction

Breastfeeding has proven over the years to be one of the most effective means to ensure child survival. The advocators and myth busters of breastfeeding myths are supposed to be health professionals. However very little is known about myths surrounding breastfeeding as expressed by these health professionals (nurses and midwives). Hence this study.

Objective

The study aimed at exploring the myths about breastfeeding as voiced by lactating nurses and midwives.

Method

Qualitative exploratory descriptive design was used to investigate the subject matter. Twenty-five nurses and midwives were purposely selected to participate in the study after they had voluntarily opted to be part of the study. Data was audiotaped, transcribed verbatim and inductively analyzed.

Results

It was evident that participants believed in myths about breastfeeding. The main themes discovered were breastfeeding myth beliefs and sociocultural myth influences. Six sub-themes identified were green stools/diarrhoea, childhood disease, teeth discoloration, child becoming stupid, culture and close relatives.

Conclusion

Health professionals believe in unfounded myths about breastfeeding. Steps should be taken for them to get better lactation support. The outcomes of this study underscore the need for health professionals to address breastfeeding myths, understand the sociocultural context, and support evidence-based practice. Improving lactation support and education for nurses and midwives can lead to better clinical practice and, as a result, help to promote successful and healthy breastfeeding in Ghana.
Examining gender effects in autistic written language skills: A small sample exploratory study
Authors: Price Johanna R , Biebesheimer Emily C , Chen Kong
Published: January 2024

Background and aims

Gender differences in the written language of autistic individuals are an overlooked but important area of research. We contend that the gender differences in spoken language of autistic individuals may extend to written language, mirroring the gender differences of writing in the general population and reflecting the shared dimensionality of oral and written language. Our research question was: Do autistic adolescent females demonstrate written language characteristics, across persuasive, expository, and narrative genres, that are distinct from those of autistic adolescent males and non-autistic (NA) adolescent females?

Methods

We performed a secondary, exploratory analysis on writing samples collected from 18 participants (11 autistic males, three autistic females, and four NA females) from a larger investigation of autistic adolescents’ writing skills. Each participant completed three writing samples—one persuasive, one expository, and one narrative (for a total of 54 writing samples). We compared sample length (total number of words), writing productivity (words written per minute), syntactic length (mean length of T-unit in words), vocabulary diversity (type-token ratio), and macrostructure of autistic females’ samples to autistic males’ and NA females’ samples.

Results

Based on non-parametric analyses using variable medians, autistic males, but not autistic females, wrote significantly shorter expository samples than NA females. Autistic males’ writing productivity was significantly lower in the persuasive and expository genres than both autistic females and NA females. Several other comparisons of sample length, productivity, vocabulary diversity, and persuasive and narrative macrostructure yielded large effect sizes but were not statistically significant.

Conclusions

Though our small sample sizes prevent us from drawing generalizable conclusions, we observed that some gender-specific findings of the current study differ from previous findings based on a single autistic group (females and males combined). Combining data of autistic females with autistic males may cloud the distinct written language characteristics of each group.

Implications

Our findings, especially when situated in the context of relevant literature, suggest that larger-scale investigation of gender differences in written language is essential in order to more fully describe the unique characteristics of autistic females. Clinicians should be prepared to support autistic writers’ needs for producing written language to meet their developmental, academic, social, and employment-related goals.
A polluting war: Risk, experts, and the politics of monitoring wartime environmental harm in Eastern Ukraine
Authors: van der Vet Freek
Published: January 2024

War always harms the environment. As the fog of war produces unreliable data, it also obstructs our capacity to monitor those harms. While some call for more data collection to advance a clear narrative of the origins of environmental harm, sociologists of risk and professional risk assessors find that the urgency of environmental hazards depends not on data alone but on who has the authority to define those risks. Without a clear understanding of how environmental experts engage in the socio-political struggles over the interpretations of risk during armed conflict, we may undervalue, first, how assessments and adequate action remain practically and politically difficult, and second, how data may be misused. Drawing on interviews with environmental experts and reports, I examine the politics of environmental expert knowledge on conflict pollution in the war in Donbas (2014–2022), a region hosting over 4500 hazardous industrial enterprises. By going beyond technological evidence collection, the article broadens our understanding of the obstacles of knowledge production and the attribution of environmental harm in highly politicized and violent contexts. Based on insights from environmental politics, the article finds that experts manage three issues undermining the reliability of environmental risks in an active warzone: pre-existing industrial pollution, environmental damage spread across government and non-government-controlled territories, and disinformation.
Qualification of geotechnical parameters with 3D geotechnical modelling to improve mine planning reliability
Authors: Aquino Eduardo da Rosa , Sotomayor Juan Manuel Girao , Torres Vidal Félix Navarro
Published: January 2024

Mine planning and pit design involve making the best decisions and recognising good practices for the profitable exploitation of mineral resources. A pit design project begins with the delimitation of the ore body in the form of blocks, then going through pit optimisation, then pit design and sequencing and ends with the economic evaluation. Several techno-economic indicators are used in these stages and have a direct impact on mine planning. The research methodology proposes an in-depth study of the geotechnical parameters of the pit to be conducted in mine planning in a more reliable and assertive way, with the aid of 3D geotechnical modelling. From a geotechnical point of view, an initial validation of the methodology was performed in an example of application in an iron mine, where a change in the planned slope angles of a pit over a 5-year period was suggested. The suggested new pit guarantees safety factors that suit the minimum stability requirements and proposes a 1.49% higher ore availability and a 0.90% increase in the net present value.
Preservice Music Teachers’ Classroom Management Beliefs
Authors: Gee Jennifer L. , Alejo Megan , Hill Jordan
Published: January 2024

The purpose of this study was to investigate the beliefs of preservice music educators regarding classroom management. Our sample consisted of 210 preservice music educators that were identified through National Association of Schools of Music–affiliated institutions. Participants completed a modified version of the Classroom Management Beliefs Survey developed by Kwok. Participants attributed the highest importance to creating a safe environment for all students and building a classroom community of care and respect for others, whereas they considered rearranging seating for desired student interactions and integrating a system of rewards and consequences as least important. There were statistically significant differences between certain categorical variables and participants’ indicated level of importance for aspects of classroom management. The implications of these findings for music teacher educators are also discussed.
Regulation of tumor metastasis and CD8+ T cells infiltration by circRNF216/miR-576-5p/ZC3H12C axis in colorectal cancer
Authors: Du Wenqi , Quan Xin , Wang Chaoqun , Song Qiuya , Mou Jie , Pei Dongsheng
Published: January 2024

Background

The tumor immune microenvironment (TIME) is an important regulator of tumor progression, growth and metastasis. In addition, tumor metastasis is one of the principal obstacles to the treatment of colorectal cancer (CRC). Circular RNAs (circRNAs) have been recognized as important regulators in the development of malignancies. However, their specific roles and mechanisms in both CRC metastasis and TIME have not been thoroughly investigated.

Methods

High-throughput next-generation sequencing technology and real-time fluorescence quantitative PCR technology were performed to identify differential circRNAs in CRC. Functional assays including transwell assay, wound healing assay, and metastasis models were conducted to assess the effect of circRNF216 on CRC metastasis. In addition, luciferase reporter, western blot, RNA immunoprecipitation (RIP), and fluorescent in situ hybridization (FISH) were performed to explore the underlying mechanism of circRNF216. The level of immune infiltration was assessed by bioinformatics analysis and flow cytometry in CRC model. Furthermore, rescue and mutation experiments were used for verification.

Results

circRNF216 was identified as a putative tumor suppressor that is downregulated in CRC tissues and cells. Overexpression of circRNF216 inhibits metastasis in vitro and vivo. Mechanistically, circRNF216 acts as a competitive endogenous RNA (ceRNA) for miR-576-5p, alleviating miR-576-5p repression on its target ZC3H12C, which in turn downregulated N-cadherin. Additionally, circRNF216 could enhance the infiltration level of CD8+ T cells by upregulating ZC3H12C, ultimately inhibiting the development of CRC, which suggests that circRNF216 is a potential biomarker for the treatment of CRC.

Conclusions

Here, we provide novel mechanistic insight revealing how circRNF216 functioned in CRC metastasis and TIME via the circRNF216/miR-576-5p/ZC3H12C pathway. Therefore, circRNF216 holds promise as a potential therapeutic target and novel diagnostic marker for CRC.
Complete chloroplast genomes of 13 species of sect. Tuberculata Chang (Camellia L.): genomic features, comparative analysis, and phylogenetic relationships
Authors: Ran Zhaohui , Li Zhi , Xiao Xu , An Mingtai , Yan Chao
Published: January 2024

Background

Sect. Tuberculata belongs to Camellia, and its members are characterized by a wrinkled pericarp and united filaments. All the plants in this group, which are endemic to China, are highly valuable for exploring the evolution of Camellia and have great potential for use as an oil source. However, due to the complex and diverse phenotypes of these species and the difficulty of investigating them in the field, their complex evolutionary history and interspecific definitions have remained largely unelucidated.

Results

Therefore, we newly sequenced and annotated 12 chloroplast (cp) genomes and retrieved the published cp genome of Camellia anlungensis Chang in sect. Tuberculata. In this study, comparative analysis of the cp genomes of the thirteen sect. Tuberculata species revealed a typical quadripartite structure characterized by a total sequence length ranging from 156,587 bp to 157,068 bp. The cp.genome arrangement is highly conserved and moderately differentiated. A total of 130 to 136 genes specific to the three types were identified by annotation, including protein-coding genes (coding sequences (CDSs)) (87–91), tRNA genes (35–37), and rRNA genes (8). The total observed frequency ranged from 23,045 (C. lipingensis) to 26,557 (C. anlungensis). IR region boundaries were analyzed to show that the ycf1 gene of C. anlungensis is located in the IRb region, while the remaining species are present only in the IRa region. Sequence variation in the SSC region is greater than that in the IR region, and most protein-coding genes have high codon preferences. Comparative analyses revealed six hotspot regions (tRNA-Thr(GGT)-psbD, psbE-petL, ycf15-tRNA-Leu(CAA), ndhF-rpl32, ndhD, and trnL(CAA)-ycf15) in the cp genomes that could serve as potential molecular markers. In addition, the results of phylogenetic tree construction based on the cp genomes showed that the thirteen sect. Tuberculata species formed a monophyletic group and were divided into two evolutionarily independent clades, confirming the independence of the section.

Conclusions

In summary, we obtained the cp genomes of thirteen sect. Tuberculata plants and performed the first comparative analysis of this group. These results will help us better characterize the plants in this section, deepen our understanding of their genetic characteristics and phylogenetic relationships, and lay the theoretical foundation for their accurate classification, elucidation of their evolutionary changes, and rational development and utilization of this section in the future.
Fine mapping and identification of two NtTOM2A homeologs responsible for tobacco mosaic virus replication in tobacco (Nicotiana tabacum L.)
Authors: Wang Xuebo , Shen Zhan , Li Caiyue , Bai Yalin , Li Yangyang , Zhang Wenhui , Li Zunqiang , Jiang Caihong , Cheng Lirui , Yang Aiguo , Liu Dan
Published: January 2024

Background

Tobacco mosaic virus (TMV) is a widely distributed viral disease that threatens many vegetables and horticultural species. Using the resistance gene N which induces a hypersensitivity reaction, is a common strategy for controlling this disease in tobacco (Nicotiana tabacum L.). However, N gene-mediated resistance has its limitations, consequently, identifying resistance genes from resistant germplasms and developing resistant cultivars is an ideal strategy for controlling the damage caused by TMV.

Results

Here, we identified highly TMV-resistant tobacco germplasm, JT88, with markedly reduced viral accumulation following TMV infection. We mapped and cloned two tobamovirus multiplication protein 2A (TOM2A) homeologs responsible for TMV replication using an F2 population derived from a cross between the TMV-susceptible cultivar K326 and the TMV-resistant cultivar JT88. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9)-mediated loss-of-function mutations of two NtTOM2A homeologs almost completely suppressed TMV replication; however, the single gene mutants showed symptoms similar to those of the wild type. Moreover, NtTOM2A natural mutations were rarely detected in 577 tobacco germplasms, and CRISPR/Cas9-mediated variation of NtTOM2A led to shortened plant height, these results indicating that the natural variations in NtTOM2A were rarely applied in tobacco breeding and the NtTOM2A maybe has an impact on growth and development.

Conclusions

The two NtTOM2A homeologs are functionally redundant and negatively regulate TMV resistance. These results deepen our understanding of the molecular mechanisms underlying TMV resistance in tobacco and provide important information for the potential application of NtTOM2A in TMV resistance breeding.
Washed microbiota transplantation improves symptoms and intestinal barrier function in patients with functional bowel disorders: a propensity-score matching analysis
Authors: Ye Xiao-Yan , Chen Jun-Yi , Wu Li-Hao , Luo Dan-Ping , Ye Xiao-Huo , Wu Li-Quan , He Xing-Xiang
Published: January 2024

Background

Alterations in the intestinal microbiota may play a role in the pathogenesis of functional bowel disorders (FBDs). Probiotics are widely used to improve intestinal dysbacteriosis in FBDs. In the context of FBDs, washed microbiota transplantation (WMT) appear to be a promising therapeutic option. We aimed to compare probiotics with WMT by using a propensity-score matching analysis (PSMA).

Methods

We conducted a retrospective investigation of 103 patients with FBDs, including irritable bowel syndrome (IBS), functional constipation (FC), functional diarrhea (FDr), functional abdominal bloating (FAB). Patients were divided into the WMT group or probiotics group (taking probiotics capsules). Data on the following parameters were matched for PSMA: age; sex; disease course; body mass index; anxiety; insomnia; tobacco smoking; alcohol consumption; and levels of D-lactate, diamine oxidase, and lipopolysaccharide. Intestinal barrier function (IBF) and symptoms were evaluated both before and after treatment initiation. Prognostic factors were assessed by Cox proportional hazards regression analysis.

Results

PSMA identified in 34 matched pairs (11 IBS, 12 FC, 7 FDr, and 4 FAB in the probiotics group and 14 IBS, 13 FC, 5 FDr, and 2 FAB in the WMT group. Improvement of FBD symptoms was greater with WMT than probiotics (P = 0.002). The WMT group had significantly fewer patients with intestinal barrier damage than the probiotics group (38.2% vs. 67.6%, P = 0.041). This improvement of FBD with WMT was further reflected as a reduction in D-lactate levels (P = 0.031). Increased D-lactate levels which were identified as a prognostic factor for FBDs (HR = 0.248, 95%CI 0.093–0.666, P = 0.006) in multivariate Cox regression analysis.

Conclusion

WMT could improve symptoms and IBF in patients with FBDs. Increased D-lactate levels in patients with FBDs may predict a favorable response to WMT treatment.
In vitro susceptibility of nontuberculous mycobacteria in China
Authors: Ren Weicong , Tan Yaoju , Ma Zichun , Shang Yuanyuan , Li Shanshan , Zhang Xuxia , Wang Wei , Yao Cong , Yuan Jinfeng , Li Liang , Pang Yu
Published: January 2024

Objectives

This study aimed to measure the prevalence of resistance to antimicrobial agents, and explore the risk factors associated with drug resistance by using nontuberculous Mycobacteria (NTM) isolates from China.

Methods

A total of 335 NTM isolates were included in our analysis. Broth dilution method was used to determine in vitro drug susceptibility of NTM isolates.

Results

Clarithromycin (CLA) was the most potent drug for Mycobacterium intracellulare (MI). The resistance rate of 244 MI isolates to CLA was 21%, yielding a minimum inhibitory concentrations (MIC)50 and MIC90 of 8 and 64 mg/L, respectively. 51% of 244 MI isolates exhibited resistance to amikacin (AMK). For 91 Mycobacterium abscessus complex (MABC) isolates, 6 (7%) and 49 (54%) isolates were categorized as resistant to CLA at day 3 and 14, respectively. The resistance rate to CLA for Mycobacterium abscessus subspecies abscessus (MAA) was dramatically higher than that for Mycobacterium abscessus subspecies massiliense (MAM). Additionally, the percentage of patients presenting fever in the CLA-susceptible group was significantly higher than that in the CLA-resistant group.

Conclusions

Our data demonstrate that approximate one fifth of MI isolates are resistant to CLA. We have identified a higher proportion of CLA-resistant MAA isolates than MAM. The patients caused by CLA-resistant MI are at low risk for presenting with fever relative to CLA-susceptible group.
Pooling sputum samples for the Xpert MTB/RIF assay: a practical screening strategy for highly infectious tuberculosis cases
Authors: Zeng Jianfeng , Huang Huan , Liu Xuhui , Huang Zhen , Liu Weijian , Liu Houming , Lu Shuihua
Published: January 2024

The Xpert MTB/RIF test (Xpert) can help in the accurate screening of tuberculosis, however, its widespread use is limited by its high cost and lack of accessibility. Pooling of sputum samples for testing is a strategy to cut expenses and enhance population coverage but may result in a decrease in detection sensitivity due to the dilution of Mycobacterium tuberculosis (Mtb) by sample mixing. We investigated how the mixing ratio affected the detection performance of Xpert. We used frozen sputum samples that had been kept after individual Xpert assays of the sputa from Mtb-confirmed TB patients and non-TB patients. Our results showed that the overall sensitivity of the Xpert pooling assay remained higher than 80% when the mixing ratio was between 1/2 and 1/8. When the mixing ratio was raised to 1/16, the positive detection rate fell to 69.0%. For patients with either a high sputum Mtb smear score ≥ 2+, a time-to-positive culture ≤ 10 days, or an Xpert test indicating a high or medium abundance of bacteria, the pooling assay positivity rates were 93.3%, 96.8%, and 100% respectively, even at a 1/16 mixing ratio. For participants with cavities and cough, the pooling assay positivity rates were 86.2% and 90.0% at a 1/8 ratio, higher than for those without these signs. Our results show that the Xpert pooled assay has a high overall sensitivity, especially for highly infectious patients. This pooling strategy with lower reagent and labor costs could support TB screening in communities with limited resources, thereby facilitating reductions in the community transmission and incidence of TB worldwide.
Changes in health facility readiness for obstetric and neonatal care services in Nepal: an analysis of cross-sectional health facility survey data in 2015 and 2021
Authors: Tuladhar Sabita , Paudel Deepak , Rehfuess Eva , Siebeck Matthias , Oberhauser Cornelia , Delius Maria
Published: January 2024

Background

Nepal is committed to achieving the Sustainable Development Goal (SDG) 2030 target 3.1 of reducing the maternal mortality ratio to 70 deaths per 100,000 live births. Along with increasing access to health facility (HF)-based delivery services, improving HF readiness is critically important. The majority of births in Nepal are normal low-risk births and most of them take place in public HFs, as does the majority of maternal deaths. This study aims to assess changes in HF readiness in Nepal between 2015 and 2021, notably, if HF readiness for providing high-quality services for normal low-risk deliveries improved; if the functionality of basic emergency obstetric and neonatal care (BEmONC) services increased; and if infection prevention and control improved.

Methods

Cross-sectional data from two nationally representative HF-based surveys in 2015 and 2021 were analyzed. This included 457 HFs in 2015 and 804 HFs in 2021, providing normal low-risk delivery services. Indices for HF readiness for normal low-risk delivery services, BEmONC service functionality, and infection prevention and control were computed. Independent sample T-test was used to measure changes over time. The results were stratified by public versus private HFs.

Results

Despite a statistically significant increase in the overall HF readiness index for normal low-risk delivery services, from 37.9% in 2015 to 43.7%, in 2021, HF readiness in 2021 remained inadequate. The availability of trained providers, essential medicines for mothers, and basic equipment and supplies was high, while that of essential medicines for newborns was moderate; availability of delivery care guidelines was low. BEmONC service functionality did not improve and remained below five percent facility coverage at both time points. In private HFs, readiness for good quality obstetrical care was higher than in public HFs at both time points. The infection prevention and control index improved over time; however, facility coverage in 2021 remained below ten percent.

Conclusions

The slow progress and sub-optimal readiness for normal, low-risk deliveries and infection prevention and control, along with declining and low BEmONC service functionality in 2021 is reflective of poor quality of care and provides some proximate explanation for the moderately high maternal mortality and the stagnation of neonatal mortality in Nepal. To reach the SDG 2030 target of reducing maternal deaths, Nepal must hasten its efforts to strengthen supply chain systems to enhance the availability and utilization of essential medicines, equipment, and supplies, along with guidelines, to bolster the human resource capacity, and to implement mechanisms to monitor quality of care. In general, the capacity of local governments to deliver basic healthcare services needs to be increased.