bluuebunny/crossref_metadata_2025 huggingface dataset:
DEVELOPMENT OF GARDEN LAND AS AN EFFORT FOR CULTIVATION OF FRUIT PLANTS IN POTS FOR PKK CADRES
Authors: Syntia Rita , Kenedi Ary Kiswanto , Asnawi
Published: February 2022

This PKM is motivated by the inadequate empowerment of PKK cadres in Aceh Tamiang Regency in utilizing the coral environment in their respective villages and the lack of maximum knowledge of cadres in cultivating potential plants through the tabulakar and tabulampot systems. The purpose of this PKM is to train PKK cadres in Aceh Tamiang Regency to maximize the potential of the yard in rural areas by using the tabulampot system so as to increase community welfare with a creative economic system. The method of implementing this PKM activity is to use the method of applying science and technology which is carried out with a system of training and mentoring for tabulampot activities. The results of PKM that have been achieved are increasing the knowledge and skills of PKK cadres in Aceh Tamiang Regency in cultivating potential plants through the system and tabulampot.
Examining the Daylighting Performance of Office Buildings on the Example of the Rectorate Building in Izmir
Authors: Kızılorenli Ecenur , Alakavuk Ebru
Published: February 2022

The efficient use of energy resources has affected all areas of life, as well as architecture. There are many ways to increase energy efficiency in buildings. Supporting the efficient use of daylight in the design process, reducing the energy to be used for artificial illumination and reducing heating costs are among the applications that support energy efficiency. The issue of supporting daylighting by reducing artificial illumination draws attention, especially in office buildings, due to the high number of hours used during the day. Insufficient light and lack of visual comfort in offices used during the day increase the consumption of electrical energy. Within the scope of this study, a parametric study was conducted to optimize the daylighting performance of an office building. For this purpose, rectorate building of a university, which is used as an office building, was selected for the case study, and the daylight condition of this building was examined. For increasing the daylighting performance of the building some revisions were proposed. Results of this research are discussed with visuals and simulation values.
The Results of the Experimental Study of the Method of Directional Radiation Pattern Transformation of a Conical Horn Radiator Operating in Millimetric (Sub-THz) Band
Authors: Astakhov Sergey P. , Stroev Nikolay N. , Yakimenko Igor V. , Sulimsky Evgeny S.
Published: February 2022

The article describes the experimental study of one of techniques of implementation of a machine-vision illuminant operating within the millimetre (sub-THz) band and forming a directional radiation pattern (DRP) with parameters compliant with the specifics of functioning of radio vision systems. In view of this, experimental research of the method of formation of a DRP configuration of a millimetre (sub-THz) machine vision system illuminant based on a conical horn radiator adapted to receiver field of view was studied experimentally using a quasi-optic system. The results of the study of the DRP configuration of such illuminant designed using a quasi-optic system installed in the aperture of a conical horn radiator are presented.
Dynamically Controlled LED Luminaire for Contrast Visualisation of Biological Tissues During Surgical Procedures
Authors: Aladov Andrey V. , Zakgeim Alexander L. , Semyashkina Julia V. , Chernyakov Anton E.
Published: February 2022

The article reviews the main theoretical, engineering and technological, circuit-engineering and software aspects of development of a dynamically controlled luminaire based on light emitting diodes for contrast visualisation of biological tissues during surgical procedures. The design concept of such surgical luminaire is proposed, which combines high-quality white lighting and coloured accent lighting increasing the contrast of visualisation of particular tissues and borders between them. The calculation model of the luminaire optical system allows maximising the level of illumination and uniformity of illuminance and colour of the surgical area. The software of the luminaire allows to independently modifying intensity of radiation of six coloured light emitting diodes with blue (460 nm), turquoise (505 nm), green (530 nm), green-yellow (550 nm), orange (590 nm) and red (630 nm) light colours for synthesis of coloured lighting of virtually any chromaticity. The level of general lighting by means of phosphor light emitting diodes can also be varied within a wide range. Chromaticity and level of lighting are adjusted by means of pulse-duration modulation of light emitting diode current and the light parameters of the luminaire are controlled by a remote computer via a radio channel.
This medical luminaire is primarily designed for lighting during surgical procedures and it can also be used for visual diagnostics based on the colour of analysed tissues.
Case Report: Our Experience with Unilateral Regional Block in a Geriatric Patient Undergoing Emergency Femoral Fracture Operation
Authors: TURAN Deniz , EPOZDEMIR Serdar
Published: February 2022

Elderly patients who face the necessity of surgery are in the group with a high potential to cause problems in the daily practice of anesthesia. The presence of cardiac, endocrine, renal, cerebral and respiratory diseases in patients in this age group increases the risk of peroperative and postoperative morbidity and mortality. In this case, we aimed to present the anesthesia management of a 110 year-old female patient who underwent emergency surgery for a femur fracture.
Circumcision of Male Children in the Community Service Project: The Characteristics and Prospective Follow up Study
Authors: Daryanto Besut , Seputra Kurnia Penta , Amorga Reza , Naim Hamdan Yuwafi
Published: February 2022

Background & Objectives: Circumcision is a procedure that involves removing the prepuce, the fold of skin covering the head of the penis. Various medical and non-medical conditions are known indications for circumcision. Various medical benefits and complications are also known to be associated with circumcision. This study aims to provide an epidemiological characteristics of the indications and complications of circumcision. Materials and Methods: This research is a prospective follow up study conducted in the context of a community service project on November 28th-29th, 2020. Circumcision was carried out according to medical and non-medical indications. Circumcision is performed by means of the dorsumcision technique. The operators were urology residents, surgery resident, and urologists. Results: There were a total of 49 participants registered in this circumcision activity, but 5 people had not passed pre-screening conditions, so they were excluded. There were 44 participants who underwent circumcision with a mean age of 10.3 years, a BMI of 19.9 and a penis length of 5.26 cm. 14 participants (31.8%) did not experience phimosis, while the rest had phimosis to varying degrees. There were 6 participants who experienced bleeding after circumcision and 5 participants who experienced delayed wound healing at 1 week post-procedure monitoring. In the second week after the procedure, all participants experienced no symptoms of complications. Conclusion: Effective and safe circumcision is performed by medical and non-medical indications. There are no long-term complications associated with circumcision.
Metastatic Colorectal Cancer: Redox Metabolism and Malignancy
Authors: AP Burlaka , OI Yevtushenko , ÐA Burlaka , OI Yatsyna , SN Lukin , II Ganusevich
Published: February 2022

Investigating the tumor microenvironment has a direct impact on the understanding of the mechanisms which regulate cancer progression and treatment. Tumor cells undergoing profound changes in their own intrinsic metabolism affect the microenvironment. Colorectal cancer (CRC) is the third most common cancer with the predominant metastases (Mts) into the liver. Mts in liver tissues (LT), adjacent to Mts (AMT) and remote tissues (RMT, taken at the distance of 5 cm from Mts) from 25 patients with metachronous liver Mts after the liver resection as well as blood and urine were studied by electron paramagnetic resonance (EPR) including the spin-trapping EPR for the detection and quantification of the activity of N2 iron-sulfur proteins, levels of NO-N2 complexes, labile iron pool (LIP), lactoferrin (Lf), superoxide and NO radicals. Activity of metalloproteinase MMP-2 and MMP-9 were determined. In adjacent and remote LT low activity of N2 in mitochondrial electron transport chain (EPR signal with g=1.94), loss of functions of detoxification system (cytochrome P450, g=2.25), appearance and growth of NO-N2 complexes (g=2.007) are obtained. Intensive EPR signals from LIP (g=2.2-2.4) and Lf (g=4.3) are registered. Superoxide generation rates were of up to 6 times higher than in the reference LT tissues and blood (p<0.001). NO levels are of 1.7 times higher for the AMT compared to RMT (p<0.05) while being 15 times higher for blood comparing to the reference species (p<0.001). Activity of MMP-2 and MMP-9 was registered both in AMT and RMT and is in 1.7 times higher in ALT (p<0.05). The obtained results can be used to estimate the functional state of organs and tissues with distant metastases, the risk of recurrence, to correct the antitumor therapeutic procedures.
The Impacts of Immunotherapy on Cancer
Authors: Morris Rachel , Scott Shayna N. , Mortimer Toni , Schekall Abigail , O’Neill Kim L.
Published: February 2022

Since the start of the 21st century, immunotherapies geared to treating cancer have continued to be refined. After a slow decline in cancer mortality rate during the last few decades, the SARS-CoV-2 pandemic is now expected to possibly increase advanced cancer stage and mortality in the future, due to delayed diagnosis and treatment resulting from recent reorganization in healthcare. Thus, the development of new cancer immunotherapies has never been more urgent. Novel research focusing on MOTO-CARs or CAR-Ms may have particular importance in revolutionizing CAR-T cell therapy. The following mini-review outlines a brief history of immunotherapy discoveries and new treatments against cancer, and reports the recent progress in checkpoint inhibitors, cancer vaccines, CAR-T cell therapies, and other major immunotherapies.
Desenvolvimento Sustentável no Brasil e o desafio da implementação da Política Nacional de Resíduos Sólidos
Authors: Almeida Rosana Santos de , Marques Agílio Tomaz , Gadelha Hugo Sarmento , Castro Filho Hiran Mendes , Maia Adryele Gomes , Santos Suzana Araújo dos , Medeiros Raquel Formiga de , Silva Matheus Matos Ferreira
Published: February 2022

A presente pesquisa tem como objetivo analisar os desafios que surgiram através da implementação da Política Nacional dos Resíduos Sólidos (PNRS) à luz do princípio do desenvolvimento sustentável. Nos últimos anos, grandes impactos têm surgido no Brasil em decorrência do aumento do consumo de produtos gerado pelo capitalismo, e consequente o descarte de resíduos sólidos vêm agravando a poluição ambiental fazendo com que o governo e a sociedade se preocupem com a forma de descarte desses resíduos. A partir dessa preocupação foi criada a Lei 12.305/2010 que deu origem à PNRS onde o art. 6º da referida lei prevê o desenvolvimento sustentável como um de seus princípios norteadores que se podem perceber por meio de diversas outras normas previstas como a gestão do ciclo dos materiais e a responsabilidade compartilhada dos poluidores. A pesquisa foi elaborada por meio da utilização do método de abordagem dedutivo, através da pesquisa bibliográfica, e documental. Através dos resultados encontrados foi possível constatar que muito embora a Lei 12.305/2010 tenha sido um passo importante na busca de soluções para o descarte de resíduos sólidos nas últimas décadas, ainda não se mostra suficiente para solucionar a questão, tendo em vista que os municípios não estão sequer colocando em prática as determinações previstas do referido dispositivo legal, sendo indispensável a ação integrada entre o governo, estados e os municípios, bem como da contribuição das empresas privadas e da população em geral.
Importância da produção de mudas para a arborização urbana: viveiros públicos de Teresina, Piauí, Brazil
Authors: Moraes Lorran André , Passos Tainara Lima de Sousa , Santos Leilson Alves dos , Lima Adriana de Sousa , Batista Waldiléia Ferreira de Melo , Santos Kelly Polyana Pereira , Machado Roselis Ribeiro Barbosa
Published: February 2022

Em face da urbanização, a sociedade tem sido afetada no que diz respeito à qualidade de vida e ao bem-estar. Preservar e integrar espécies vegetais no ambiente urbano tem sido sinônimo de sobrevivência. Dessa forma, os viveiros de mudas se apresentam como elemento essencial para a arborização urbana.  Neste sentido, o presente trabalho apresenta informações de dois viveiros de mudas da cidade de Teresina, capital do Piauí, apresentando um diagnóstico acerca de suas dinâmicas, compreendendo como são realizadas cada etapa e fases do processo de coleta, armazenamento e beneficiamento das sementes, produção, manejo, controle, identificação e distribuição das mudas. Apresenta-se, ainda, dados sobre a rotina de produção de mudas e os processos que envolvem desde a coleta das sementes a sua distribuição à população. A abordagem técnica da pesquisa é qualitativa, embasada na pesquisa bibliográfica, de campo e a documental. Nessa pesquisa, observou-se que são produzidas nos viveiros mudas de aproximadamente 80 espécies botânicas. Os resultados demonstram a importância desses locais possuem para a população, a Secretaria de Meio Ambiente e para a comunidade cientifica, sendo ainda, de grande relevância para o planejamento futuro. Portanto, arborizar vai além de plantar mudas em vários lugares, requer planejamento bem gerenciado, assim o conhecimento da dinâmica em viveiros de mudas bem como o repasse dessas informações gera condições de segurança na qualidade e no bem-estar das futuras gerações.
Antibacterial activity of Kombucha against Escherichia coli and Staphylococcus aureus and Minas Frescal cheese production with Scoby
Authors: Coelho Eduardo Bergamaski , Saraiva Bruna Barnei , Rodrigues Bruna Moura , Silva Júnior Ranulfo Combuca da , Campanholi Katieli da Silva Souza , Maricato Dayse Maria Bernardo , Pintro Paula Matumoto , Pozza Magali Soares dos Santos
Published: January 2022

Functional ingredients have become common in food products. The fermented Kombucha drink stands out for its probiotic and antimicrobial properties that beneficially contribute to food use. The present study aimed at assessing the antimicrobial activity of Kombucha in experimentally contaminated cheeses. Therefore, Minas Frescal cheeses were prepared, inoculated with Escherichia coli and divided into three treatments:  1) lactic acid bacteria (BioRich®); 2) lactic bacteria (BioRich ®) and 10 g of Scoby (symbiotic culture of bacteria and yeasts); 3) 10 g of Scoby. The gelatinous film (Scoby) was used to produce the Kombucha extract. The experimental design was a 3 x 4 factorial arrangement with three treatments and four evaluation times. Data was analyzed with ANOVA, and treatments means were compared with the Tukey test (p £ 0.05). The parameters of minimum inhibitory concentration, color, texture, pH, acidity, water activity, counting of yeasts, lactobacilli, and total coliforms were evaluated on days 0, 3, 6, and 9 of storage. Antibacterial action of Kombucha was compared against Escherichia coli and Staphylococcus aureus bacteria for minimum inhibitory concentration. Acidity, bacterial counting, yeasts, coliforms, lactobacilli, hardness, and gumminess showed significant changes with the addition of Kombucha to cheeses (p £ 0.05). However, However, the addition of Kombucha to the cheeses did not reduce the coliform content and negatively altered the texture parameters.
Análise da influência da mídia nas decisões do Tribunal do Júri
Authors: Almeida Rosana Santos de , Maciel Jonatas Claudio Farias , Medeiros Raquel Formiga de , Gadelha Hugo Sarmento , Castro Filho Hiran Mendes , Santos Suzana Araújo dos , Varejão Marcela da Silva , Marques Agílio Tomaz
Published: February 2022

A informação jornalística, apreciada por grande parte da população tem o dever de levar a notícia dos fatos ocorridos na sociedade, sua função primeira é de transmitir com autenticidade a informação. Com garantias constitucionais, amparada nas liberdades de opinião e pensamento a mídia livre é o pleno sinal de uma democracia forte e coesa, no entanto o poder de manobra de tais meios construindo um juízo de valor na sociedade implica diretamente interferindo no Tribunal do Júri, cujos jurados são membros da sociedade. Dessa forma, o presente estudo se consolida na análise da liberdade de imprensa na legislação pátria, enfocando como a mídia interfere nas informações de ordem criminal através de sua cobertura sensacionalista, realizando um pré-julgamento do acusado e interferindo na decisão do Júri Popular. Neste sentido evocaram-se formas que garantem a liberdade de informação e ao mesmo tempo demonstram meios de se coibir os excessos e se restabelecer a verdade dos fatos. Na realização da pesquisa, foi empregado o método de abordagem dedutivo e métodos de procedimento científico, com as regras e normas seguidas pelo rigor da ciência, histórico na avaliação dos institutos jurídicos surgidos, comparativo na explicação dos fenômenos ou fatos constantes, monográfico estudando o caso com profundidade, a fim de ilustrar o papel dos meios de comunicação interessados na audiência pela ampla veiculação, muitas vezes de forma destorcida, de informações criminais que comovem a população.
Dermatophytosis by Trichophyton verrucosum in domestic feline – case report
Authors: Melo Danilo Rocha de , Santos Juliany Nunes dos , Rocha Evelyn Carla da Frota , Oliveira Angélica Prado de , Santos Jôiciglecia Pereira dos , Souza Valesca Ferreira Machado de , Rodrigues Valquíria Tatiele da Silva , Vieira Layze Cilmara Alves da Silva
Published: February 2022

The present study aimed to report a case of dermatophytosis caused by Trichophyton verrucosum in a domestic feline. The patient was admitted to the University Veterinary Hospital of the Federal University of West Bahia, with dermatological complaints. On physical examination, alopecic lesions with an erythematous, hyperpigmented appearance and the presence of papules were noted, which were distributed throughout the body, with a greater predominance in the thoracic limbs, ventral region and head. Dermatological samples were collected from different areas of the body with lesions, which were sent for mycological culture and research for ectoparasites at the Laboratory of Clinical Analysis and Veterinary Microbiology of the same institution. The fungal examination showed the growth of colonies with a prominent center and flat periphery with salmon coloring and a subtly granular texture, demonstrating on microscopy hyaline septate fungal filaments, the presence of few piriform to ovoid shaped microconidia, in addition to several chlamydospores and hyphae with engorgement. terminal, compatible with “pearl chains”, confirming a case of dermatophytosis by T. verrucosum. The literature approaches this fungus as a cause of disease in cattle and other ruminants, however there is still little information about its distribution and epidemiology in felines in Brazil, revealing the need for epidemiological studies in the area. It is concluded, therefore, that T. verrucosum infection in felines should be considered as a differential diagnosis of other dermatological conditions in the small animal clinic.
Efeitos da Musicoterapia aplicada às crianças com condições complexas crônicas
Authors: Bubadué Renata de Moura , Reis Fabiano Pereira dos , Silva Francisco das Chagas dos Santos da , Santos Erika Eberlline Pacheco dos , Primeira Marcelo Ribeiro
Published: February 2022

Este artigo tem como objetivo descrever os efeitos da musicoterapia aplicada a crianças com condições crônicas complexas sob a ótica do familiar. Pesquisa interventiva e participativa realizada com onze crianças com condições crônicas complexas de saúde no período de março a abril de 2021 em uma cidade de médio porte do centro-oeste do Brasil. A pesquisa foi aprovada pelo Comitê de Ética sob o CAAE 41238820.1.0000.5595. Segundo os familiares, houve mudança de comportamento durante e após as sessões de musicoterapia. A musicoterapia melhorou a comunicação, o foco, a interação, o relaxamento e o vínculo das crianças com os profissionais de saúde e familiares.
Estudo de viabilidade econômica do uso de mictório seco
Authors: Carvalho Matheus Vieira , Morais Lucas Barros Figueiredo de , Farto Cindy Deina , Athayde Junior Gilson Barbosa
Published: February 2022

É inegável que a escassez hídrica vem causando racionamentos em alguns grandes centros urbanos brasileiros. Visto isso, a utilização de aparelhos economizadores de água surge como uma das alternativas para a conservação e uso eficiente desse recurso, dentre eles, destacam-se os mictórios secos, que, apesar dos custos de aquisição e de operação, trazem o benefício da economia de água. Este artigo propôs analisar a viabilidade econômica do uso de mictórios secos, para um tempo de vida útil de projeto de 20 anos, com dados referentes ao município de João Pessoa – PB, comparando-os com três tipos de aparelhos sanitários: mictório convencional (cenário 1), bacia sanitária de acionamento duplo (cenário 2) e bacia sanitária de acionamento único (cenário 3). Na categoria residencial, a utilização do mictório seco não foi analisada para o cenário 1, enquanto, no cenário 2, a viabilidade ocorre a partir de 20 usos diários para a tarifação intermediária e 7 usos diários para a tarifa de residências de padrão alto. Já no cenário 3, a viabilidade é verificada a partir de 7, 4 e 3 usos diários para as tarifas de padrão popular, médio e alto, respectivamente. Nas categorias comercial, industrial e pública, os mictórios secos mostram-se viáveis economicamente nos cenários em que substituem as bacias sanitárias. No cenário 2, os números de usos diários para que ocorra a viabilidade econômica foram a partir de 5, 4 e 4, no cenário 3, 2, 2 e 2 para as categorias comercial, industrial e pública, respectivamente.
Avaliação do nível de conhecimento da prescrição de AINEs para equinos no nordeste brasileiro
Authors: Santos Júnior Dinamérico de Alencar , Carvalho Caio Victor Damasceno , Bomfim Felipe Pires de Souza , Pereira Matheus Teixeira Borges , Carvalho Saulo de Almeida , Miranda Neto Eldinê Gomes de , Escodro Pierre Barnabé
Published: February 2022

No presente estudo foram aplicados 100 questionários (50 por grupo) com o objetivo de identificar quais os principais AINEs recomendados por médicos veterinários (G1) e vendedores/balconistas de lojas agropecuárias (G2) na região Nordeste do Brasil para o tratamento das afecções dos sistemas locomotor e digestório da espécie equina.  Para as afecções do sistema locomotor: como primeira opção, a fenilbutazona foi descrita como principal AINE nos dois grupos (G1: 58% e G2: 28%). Como segunda opção, o flunixin meglumine foi o mais recomendado (G1: 32% e G2: 24%). Como terceira opção, o meloxicam foi o mais recomendado também por ambos (G1:26% e G2: 16%). Para as afecções do sistema digestório: como primeira opção, o flunixin meglumine foi o fármaco mais recomendado (G1: 54% e G2: 40%). Como segunda opção, para o G1, flunixin meglumine (36%) e para o G2, a dipirona (30%). Como terceira opção, para o G1, o meloxicam e o DMSO foram os mais citados (18% cada) e para o G2, a fenilbutazona (18%). Os AINEs não seletivos são os mais recomendados por veterinários e vendedores de lojas agropecuárias do Nordeste do Brasil para tratamento das afecções locomotoras e digestivas em equinos, enquanto que os inibidores seletivos para COX-2 ainda são pouco recomendados. O desconhecimento das dosagens técnicas foi constatado em ambos os grupos, com destaque para o G2. Medidas de controle na venda de AINEs e orientação sobre seu uso correto aos profissionais do cavalo fazem-se necessárias.
A reciclagem dos resíduos plásticos de Manaus (AM): O caso das entidades de catadores
Authors: Silvestrim Eneida Guerra , Rivas Alexandre Almir Ferreira , Vieira Michael Raphael Soares , Santana Genilson Pereira
Published: February 2022

Apesar da Política Nacional de Resíduos Sólidos no Brasil ser bem instituída, o país reaproveita muito pouco os seus resíduos sólidos urbanos, como papel, plástico, entre outros; ou seja, a maior parte é despejada em aterros e locais inadequados. No caso da região Norte com área de 45,2 do território brasileiro e com a maior diversidade do mundo, o mercado de recicláveis ainda é baixo, principalmente na cidade de Manaus. Portanto, neste trabalho as entidades de catadores de Manaus que trabalham com materiais plásticos recicláveis foram estudadas a fim de mostrar um diagnóstico da reciclagem de resíduos plásticos. Dessa forma, um questionário contendo perguntas sobre o processo de funcionamento das entidades de catadores foi aplicado. O espaço amostral deste estudo constou de sete entidades de catadores para um erro experimental de 2,5%. Os resultados deste estudo mostram que os resíduos plásticos não são a principal fonte de renda entre os materiais recicláveis das entidades de catadores de Manaus. Além disso, as entidades de catadores e o poder público não trocam informações sobre os resíduos sólidos urbano da cidade de Manaus, o que dificulta o processo de reciclagem, principalmente de resíduos plásticos. Considerando o volume total de resíduos sólidos plásticos de 91.080 kg, vendido ao ano temos uma receita total de R$ 213.072,00/ano. Apenas para o resíduo plástico. A falta de sincronização de informação e gestão entre o poder público e as entidades de catadores explicam em parte a quantidade de apenas cerca de 0,27% de resíduos sólidos urbanos sendo reciclados na cidade de Manaus. Por outro lado, a reciclagem dos resíduos plásticos é uma alternativa para a redução das emissões de efeito estufa e pode ser fonte de créditos de carbono, trazendo benefícios para a Amazônia
Laboratórios on-line: Espaços do ensino remoto e possíveis contribuições para formação humana integral na educação básica
Authors: Costa Fernanda Alves , Maldaner Jair José , Rythowem Marcelo , Cavalcante Rivadavia Porto , Sena Rosa Maria Machado de , Victor Valcí Ferreira , Monteiro Claudio de Castro , Souza Wallysonn Alves de
Published: February 2022

O uso das Tecnologias de Informação e Comunicação (TICs) tornou-se essencial nas atividades das instituições de educação por meio do ensino remoto com a suspensão das aulas presenciais por causa da pandemia da Covid-19. Devido às dificuldades de acesso aos laboratórios tradicionais para realização de práticas pelos estudantes da educação básica, tornou-se essencial a utilização de laboratórios remotos e virtuais para realização de práticas, que podem ser acessados via internet independente da localização geográfica dos estudantes, uma série destes têm sido desenvolvidos por algumas instituições, destaca-se a Universidade Federal de Santa Catarina (UFSC) através do grupo de pesquisa do Laboratório de Experimentação Remota (RexLab). Esse novo contexto passou a exigir pesquisas sobre projetos voltados para integração das TICs na educação básica sob a perspectiva da formação humana integral. Este artigo tem como objetivo de relatar sobre experiências exitosas dos projetos voltados para uso de tecnologias educacionais que utilizam os laboratórios remotos e virtuais voltado para o ensino médio no intuito de aplicação de práticas de disciplinas das ciências aplicadas, desenvolvido pelo grupo de pesquisa do RExLab da UFSC com apoio do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ).
Representações sociais de acadêmicos de Enfermagem sobre o câncer do colo do útero
Authors: Silva Maicla Oliveira da , Santos Charles Souza , Bastos Norma Lopes de Magalhaes Velasco , Machado Juliana Costa , Oliveira Zulmerinda Meira , Melo Angelle Matias , Sousa Micaela Leão de , Souza Evelin Matos Marambaia , Ribeiro Marcela Rossi , Santos Maycon Brandão dos , Farias Anadir de Almeida , Barreto Rebecca Calheira , Leal Letícia Souza , Lôbo Marcio Pereira , Gomes Antonio Marcos Tosoli
Published: February 2022

Objetivo: Analisar a estrutura representacional dos acadêmicos de enfermagem sobre o câncer do colo do útero. Metodologia: estudo qualitativo sustentado na Teoria das Representações Sociais, em sua abordagem estrutural, realizada com 57 acadêmicos do 1º ao 6º semestre do curso de Enfermagem da Universidade Estadual do Sudoeste da Bahia, campus de Jequié-Ba. Dados coletados por questionário de evocações livres ao termo indutor câncer do colo do útero, por meio de formulário produzido na plataforma google forms e analisados pela técnica do quadrante de quatro casas, auxílio do software Ensemble de Programmes Permettant l’analyse dês Evocations 2005 e pela análise de similitude. Resultados: Evidenciaram uma estrutura representacional dos acadêmicos de enfermagem sobre o câncer do colo do útero constituída por três dimensões: individual, social e imagética que foram gerenciadas por um núcleo central formado pelos cognemas papanicolau, prevenção e tratamento. As dimensões e o núcleo central desta estrutura representacional demonstraram os conhecimentos socialmente elaborados pelos participantes. Conclusão: A estrutura representacional apresenta uma possível demonstração do conhecimento dos futuros profissionais de enfermagem, abrindo espaço para criação de práticas colaborativas que contribuirá ainda mais para seu desenvolvimento dentro e fora da instituição, abordando a importância da saúde da mulher, controle e prevenção do câncer do colo do útero e demais cuidados, onde o enfermeiro possui fundamental colaboração.
Imunocards: aprendendo as defesas do organismo jogando
Authors: Vale Filho Maurício de Almeida , Santos Pablo de Castro , Maia Allyssandra Maria Lima Rodrigues
Published: February 2022

O estudo da Imunologia revela a importância de se conhecer a atuação do Sistema Imunológico nas defesas do organismo e sua habilidade de resposta à ação de substâncias imuno estimulantes e/ou invasão de microrganismos do ambiente. Esse conteúdo deve ser ministrado na disciplina de Biologia, sobretudo por entender-se, que na educação básica, a formação conceitual e o estabelecimento de relações entre estes conceitos são primordiais para a formação do indivíduo.  Nessa perspectiva, o trabalho teve como objetivo desenvolver um jogo de cartas do tipo baralho, de caráter lúdico, educativo e investigativo e inseri-lo no contexto do ensino da Imunologia para alunos do ensino médio, de uma escola estadual do município de Russas-CE. Metodologias diversificadas como o uso de jogos tem elevada aceitação dentre os estudantes, e estes, mediante a atividade, demonstram melhor compreensão global dos temas tratados na disciplina.  A pesquisa foi realizada com base no método interpretativo em consonância com a abordagem quali-quantitativa. A análise interpretativa dos dados qualitativos revelaram uma boa percepção dos estudantes acerca do tema proposto, logo, os mesmos apresentaram conhecimentos prévios importantes para uma boa compreensão do conteúdo nas aulas de Biologia. Os resultados quantitativos por sua vez evidenciaram que o jogo IMUNOCARDS auxiliou na compreensão do estudo da Imunologia, bem como, facilitou a aquisição de informações e a produção de novos conhecimentos. Os dados permitiram ainda, perceber que o jogo se mostrou uma ferramenta criativa, agradável, atraente, lúdica e inovadora no ensino de Biologia.
Feeling Like a Fraud: Imposter Syndrome in Political Science
Authors: Benson Thomas , Gentry Bobbi , Shugars Sarah
Published: February 2022

Imposter syndrome can present feelings of intellectual self-doubt – among other things – and it is not experienced equally or in a universal manner, with intersectional identities able to shape and compound how imposter syndrome is experienced. This chapter offers numerous recommendations to help manage imposter syndrome for oneself or to help others. This manuscript is part of Strategies for Navigating Graduate School and Beyond, a forthcoming volume for those interested in pursuing graduate education in political science (Fall 2022 publication).
State intervention and the labyrinth of communal conflict in Ghana: How Kwame Nkrumah missed the opportunity to address the Bawku conflict
Authors: Dramani Aminu , SEBASTIAN PAALO
Published: February 2022

Most scholarly accounts assert that governments’ conflict resolution and peacebuilding efforts fail to address the root causes, prolong, or worsen the conflict situation. This supposed failure arises mainly because state actors pursue strategic interests in the peace processes, which provokes adversarial inter-and intra-group relations, especially in Africa where communal conflicts remain a great threat to economies and security. Nonetheless, the discussions fail to adequately investigate the details of state interventions and how they affect the potential for peace in some notorious conflict-affected areas. This paper examines how the Opoku-Afari Committee Report, the government of Ghana’s first official response to the Bawku conflict in 1957, protracts and further complicates the conflict. findings further the existing knowledge on the conflict by drawing out specifics of the state failure which are usually obscured in the literature.
Assessment of the Effect of the Rate of Internal Displaced Persons on Food Price Variations Time Series from Year 2009 to 2020 in Badakhshan, Afghanistan.
Authors: Uba Matthew Ndubuisi
Published: February 2022

Internally displacement of persons is a major factor leading to food insecurity in several countries. Displacement is caused by conflict or environmental disaster. Displacement leads to less production of agricultural produce resulting to food prices increases. Secondary data was analyzed using R-Programming, Variables such as Year (2009 to 2020), Food Price Index from 2009 to 2020, Conflict stock displacement from 2009 to 2020 and Environmental Disaster Displacement from 2009 to 2020 were extracted from the dataset for analysis. Their trends were observed using time series. Regression results revealed that food price was increasing significantly with respect to time with a p-value of 2.2e-16 <0.05. Also, the internally displaced persons caused by conflict has a significant impact on the increasing food price with a p-value of 7.137e-08 <0.05, but the internally displaced persons caused by environmental disaster has no significant impact on the increasing food price.
Maternal Low-Protein Diet Deregulates DNA Repair and DNA Replication Pathways in Female Offspring Mammary Gland Leading to Increased Chemically Induced Rat Carcinogenesis in Adulthood
Authors: Zapaterini Joyce R. , Fonseca Antonio R. B. , Bidinotto Lucas T. , Colombelli Ketlin T. , Rossi André L. D. , Kass Laura , Justulin Luis A. , Barbisan Luis F.
Published: February 2022

Studies have shown that maternal malnutrition, especially a low-protein diet (LPD), plays a key role in the developmental mechanisms underlying mammary cancer programming in female offspring. However, the molecular pathways associated with this higher susceptibility are still poorly understood. Thus, this study investigated the adverse effects of gestational and lactational low protein intake on gene expression of key pathways involved in mammary tumor initiation after a single dose of N-methyl-N-nitrosourea (MNU) in female offspring rats. Pregnant Sprague–Dawley rats were fed a normal-protein diet (NPD) (17% protein) or LPD (6% protein) from gestational day 1 to postnatal day (PND) 21. After weaning (PND 21), female offspring (n = 5, each diet) were euthanized for histological analysis or received NPD (n = 56 each diet). At PND 28 or 35, female offspring received a single dose of MNU (25 mg/kg body weight) (n = 28 each diet/timepoint). After 24 h, some females (n = 10 each diet/timepoint) were euthanized for histological, immunohistochemical, and molecular analyses at PDN 29 or 36. The remaining animals (n = 18 each diet/timepoint) were euthanized when tumors reached ≥2 cm or at PND 250. Besides the mammary gland development delay observed in LPD 21 and 28 groups, the gene expression profile demonstrated that maternal LPD deregulated 21 genes related to DNA repair and DNA replication pathways in the mammary gland of LPD 35 group after MNU. We further confirmed an increased γ-H2AX (DNA damage biomarker) and in ER-α immunoreactivity in mammary epithelial cells in the LPD group at PND 36. Furthermore, these early postnatal events were followed by significantly higher mammary carcinogenesis susceptibility in offspring at adulthood. Thus, the results indicate that maternal LPD influenced the programming of chemically induced mammary carcinogenesis in female offspring through increase in DNA damage and deregulation of DNA repair and DNA replication pathways. Also, Cidea upregulation gene in the LPD 35 group may suggest that maternal LPD could deregulate genes possibly leading to increased risk of mammary cancer development and/or poor prognosis. These findings increase the body of evidence of early-transcriptional mammary gland changes influenced by maternal LPD, resulting in differential response to breast tumor initiation and susceptibility and may raise discussions about lifelong prevention of breast cancer risk.
Visual Function is Gradually Restored During Retina Regeneration in Adult Zebrafish
Authors: Hammer Juliane , Röppenack Paul , Yousuf Sarah , Schnabel Christian , Weber Anke , Zöller Daniela , Koch Edmund , Hans Stefan , Brand Michael
Published: February 2022

In comparison to mammals, zebrafish are able to regenerate many organs and tissues, including the central nervous system (CNS). Within the CNS-derived neural retina, light lesions result in a loss of photoreceptors and the subsequent activation of Müller glia, the retinal stem cells. Müller glia-derived progenitors differentiate and eventually restore the anatomical tissue architecture within 4 weeks. However, little is known about how light lesions impair vision functionally, as well as how and to what extent visual function is restored during the course of regeneration, in particular in adult animals. Here, we applied quantitative behavioral assays to assess restoration of visual function during homeostasis and regeneration in adult zebrafish. We developed a novel vision-dependent social preference test, and show that vision is massively impaired early after lesion, but is restored to pre-lesion levels within 7 days after lesion. Furthermore, we employed a quantitative optokinetic response assay with different degrees of difficulty, similar to vision tests in humans. We found that vision for easy conditions with high contrast and low level of detail, as well as color vision, was restored around 7–10 days post lesion. Vision under more demanding conditions, with low contrast and high level of detail, was regained only later from 14 days post lesion onwards. Taken together, we conclude that vision based on contrast sensitivity, spatial resolution and the perception of colors is restored after light lesion in adult zebrafish in a gradual manner.
Emerging Roles of COX7RP and Mitochondrial Oxidative Phosphorylation in Breast Cancer
Authors: Kamada Shuhei , Takeiwa Toshihiko , Ikeda Kazuhiro , Horie Kuniko , Inoue Satoshi
Published: February 2022

Metabolic alterations are critical events in cancers, which often contribute to tumor pathophysiology. While aerobic glycolysis is a known characteristic of cancer-related metabolism, recent studies have shed light on mitochondria-related metabolic pathways in cancer biology, including oxidative phosphorylation (OXPHOS), amino acid and lipid metabolism, nucleic acid metabolism, and redox regulation. Breast cancer is the most common cancer in women; thus, elucidation of breast cancer-related metabolic alteration will help to develop cancer drugs for many patients. We here aim to define the contribution of mitochondrial metabolism to breast cancer biology. The relevance of OXPHOS in breast cancer has been recently defined by the discovery of COX7RP, which promotes mitochondrial respiratory supercomplex assembly and glutamine metabolism: the latter is also shown to promote nucleic acid and fatty acid biosynthesis as well as ROS defense regulation. In this context, the estrogen-related receptor (ERR) family nuclear receptors and collaborating coactivators peroxisome proliferator-activated receptor-γ coactivator-1 (PGC-1) are essential transcriptional regulators for both energy production and cancer-related metabolism. Summarizing recent findings of mitochondrial metabolism in breast cancer, this review will aim to provide a clue for the development of alternative clinical management by modulating the activities of responsible molecules involved in disease-specific metabolic alterations.
Ruvbl2 Suppresses Cardiomyocyte Proliferation During Zebrafish Heart Development and Regeneration
Authors: Sharpe Michka , González-Rosa Juan Manuel , Wranitz Felicia , Jeffrey Spencer , Copenhaver Katherine , Burns C. Geoffrey , Burns Caroline E.
Published: February 2022

Cardiomyocyte proliferation is an important source of new myocardium during heart development and regeneration. Consequently, mutations in drivers of cardiomyocyte proliferation cause congenital heart disease, and infarcted human hearts scar because cardiomyocytes exit the cell cycle postnatally. To boost cardiomyocyte proliferation in either setting, critical regulators must be identified. Through an ENU screen in zebrafish, the liebeskummer (lik) mutant was isolated and described as having elevated cardiomyocyte numbers during embryogenesis. The lik mutation results in a three amino acid insertion into Ruvbl2, a highly conserved ATPase. Because both gain- and loss-of-function properties have been described for ruvbl2lik, it remains unclear whether Ruvbl2 positively or negatively regulates cardiomyocyte proliferation. Here, we demonstrate that Ruvbl2 is a suppressor of cardiomyocyte proliferation during zebrafish heart development and regeneration. First, we confirmed speculation that augmented cardiomyocyte numbers in ruvbl2lik/lik hearts arise by hyperproliferation. To characterize bona fide ruvbl2 null animals, we created a ruvbl2 locus deletion allele (ruvbl2Δ). Like ruvbl2lik/lik mutants, ruvbl2Δ/Δ and compound heterozygote ruvbl2lik/Δ animals display ventricular hyperplasia, demonstrating that lik is a loss of function allele and that ruvbl2 represses cardiomyocyte proliferation. This activity is autonomous because constitutive myocardial overexpression of Ruvbl2 is sufficient to suppress cardiomyocyte proliferation in control hearts and rescue the hyperproliferation observed in ruvbl2Δ/Δ mutant hearts. Lastly, heat-shock inducible overexpression of Ruvbl2 suppresses cardiomyocyte proliferation during heart regeneration and leads to scarring. Together, our data demonstrate that Ruvbl2 functions autonomously as a suppressor of cardiomyocyte proliferation during both zebrafish heart development and adult heart regeneration.
Discovered Key CpG Sites by Analyzing DNA Methylation and Gene Expression in Breast Cancer Samples
Authors: Cao Yan-Ni , Li Qian-Zhong , Liu Yu-Xian
Published: February 2022

Breast cancer is the most common cancer in the world, and DNA methylation plays a key role in the occurrence and development of breast cancer. However, the effect of DNA methylation in different gene functional regions on gene expression and the effect of gene expression on breast cancer is not completely clear. In our study, we computed and analyzed DNA methylation, gene expression, and clinical data in the TCGA database. Firstly, we calculated the distribution of abnormal DNA methylated probes in 12 regions, found the abnormal DNA methylated probes in down-regulated genes were highly enriched, and the number of hypermethylated probes in the promoter region was 6.5 times than that of hypomethylated probes. Secondly, the correlation coefficients between abnormal DNA methylated values in each functional region of differentially expressed genes and gene expression values were calculated. Then, co-expression analysis of differentially expressed genes was performed, 34 hub genes in cancer-related pathways were obtained, of which 11 genes were regulated by abnormal DNA methylation. Finally, a multivariate Cox regression analysis was performed on 27 probes of 11 genes. Three DNA methylation probes (cg13569051 and cg14399183 of GSN, and cg25274503 of CAV2) related to survival were used to construct a prognostic model, which has a good prognostic ability. Furthermore, we found that the cg25274503 hypermethylation in the promoter region inhibited the expression of the CAV2, and the hypermethylation of cg13569051 and cg14399183 in the 5′UTR region inhibited the expression of GSN. These results may provide possible molecular targets for breast cancer.
The Characterization of Structure and Prediction for Aquaporin in Tumour Progression by Machine Learning
Authors: Chen Zheng , Jiao Shihu , Zhao Da , Zou Quan , Xu Lei , Zhang Lijun , Su Xi
Published: February 2022

Recurrence and new cases of cancer constitute a challenging human health problem. Aquaporins (AQPs) can be expressed in many types of tumours, including the brain, breast, pancreas, colon, skin, ovaries, and lungs, and the histological grade of cancer is positively correlated with AQP expression. Therefore, the identification of aquaporins is an area to explore. Computational tools play an important role in aquaporin identification. In this research, we propose reliable, accurate and automated sequence predictor iAQPs-RF to identify AQPs. In this study, the feature extraction method was 188D (global protein sequence descriptor, GPSD). Six common classifiers, including random forest (RF), NaiveBayes (NB), support vector machine (SVM), XGBoost, logistic regression (LR) and decision tree (DT), were used for AQP classification. The classification results show that the random forest (RF) algorithm is the most suitable machine learning algorithm, and the accuracy was 97.689%. Analysis of Variance (ANOVA) was used to analyse these characteristics. Feature rank based on the ANOVA method and IFS strategy was applied to search for the optimal features. The classification results suggest that the 26th feature (neutral/hydrophobic) and 21st feature (hydrophobic) are the two most powerful and informative features that distinguish AQPs from non-AQPs. Previous studies reported that plasma membrane proteins have hydrophobic characteristics. Aquaporin subcellular localization prediction showed that all aquaporins were plasma membrane proteins with highly conserved transmembrane structures. In addition, the 3D structure of aquaporins was consistent with the localization results. Therefore, these studies confirmed that aquaporins possess hydrophobic properties. Although aquaporins are highly conserved transmembrane structures, the phylogenetic tree shows the diversity of aquaporins during evolution. The PCA showed that positive and negative samples were well separated by 54D features, indicating that the 54D feature can effectively classify aquaporins. The online prediction server is accessible at http://lab.malab.cn/∼acy/iAQP.