bluuebunny/crossref_metadata_2025 huggingface dataset:
Characteristics of expression and regulation of sirtuins in chicken (Gallus gallus)
Authors: Ren Junxiao , Xu Naiyi , Ma Zheng , Li Yanmin , Li Cuicui , Wang Yanbin , Tian Yadong , Liu Xiaojun , Kang Xiangtao
Published: November 2016

Sirtuins (SIRT1–SIRT7) are a family of NAD+-dependent protein deacetylases that are linked to post-translational regulation of many metabolic processes. There are few reports available for chicken sirtuins (designated cSIRT1–cSIRT7), whose expression and regulation in the liver have yet to be explored. In the present study, we characterized the expression and regulation of sirtuin family members in chicken liver. The results showed that the sirtuin family members in chicken share the same conserved functional SIR2 domains. All the sirtuin family members were expressed extensively in all tissues examined, and the expression levels of cSIRT1, cSIRT2, cSIRT4, cSIRT6, and cSIRT7 in the liver increased significantly with sexual maturity. However, all sirtuin family members were downregulated (P < 0.05) in chicken livers and cultured primary hepatocytes treated with 17β-estradiol. We concluded that the expression levels of some chicken sirtuin family members in the liver were upregulated with sexual maturation, but might not be regulated directly by estrogen. Whereas estrogen could be used as an inhibitor of all sirtuins, both in vivo and in vitro.
On the consistency problem for modular lattices and related structures
Authors: Herrmann Christian , Tsukamoto Yasuyuki , Ziegler Martin
Published: November 2016

The consistency problem for a class of algebraic structures asks for an algorithm to decide, for any given conjunction of equations, whether it admits a non-trivial satisfying assignment within some member of the class. For the variety of all groups, this is the complement of the triviality problem, shown undecidable by by Adyan [Algorithmic unsolvability of problems of recognition of certain properties of groups. (Russian) Dokl. Akad. Nauk SSSR (N.S.) 103 (1955) 533–535] and Rabin [Recursive unsolvability of group theoretic problems, Ann. of Math. (2) 67 (1958) 172–194]. For the class of finite groups, it amounts to the triviality problem for profinite completions, shown undecidable by Bridson and Wilton [The triviality problem for profinite completions, Invent. Math. 202 (2015) 839–874]. We derive unsolvability of the consistency problem for the class of (finite) modular lattices and various subclasses; in particular, the class of all subspace lattices of finite-dimensional vector spaces over a fixed or arbitrary field of characteristic [Formula: see text] and expansions thereof, e.g. the class of subspace ortholattices of finite-dimensional Hilbert spaces. The lattice results are used to prove unsolvability of the consistency problem for (finite) rings with unit and (finite) representable relation algebras. These results in turn apply to equations between simple expressions in Grassmann–Cayley algebra and to functional and embedded multivalued dependencies in databases.
Relative Gorenstein global dimension
Authors: Bennis Driss , García Rozas J. R. , Oyonarte Luis
Published: November 2016

We study the relative Gorenstein projective global dimension of a ring with respect to a weakly Wakamatsu tilting module [Formula: see text]. We prove that this relative global dimension is finite if and only if the injective dimension of every module in Add[Formula: see text] and the [Formula: see text]-projective dimension of every injective module are both finite (indeed these three dimensions have a common upper bound). When RC satisfies some extra conditions we prove that the relative Gorenstein projective global dimension of [Formula: see text] is always bounded above by the [Formula: see text]-projective global dimension of [Formula: see text], these two dimensions being equal when the class of all [Formula: see text]-Gorenstein projective [Formula: see text]-modules is contained in the Bass class of [Formula: see text] relative to [Formula: see text]. Of course we also give the dual results concerning the relative Gorenstein injective global dimension.
Stochastic spatial model for the division of labor in social insects
Authors: Arcuri Alesandro , Lanchier Nicolas
Published: November 2016

Motivated by the study of social insects, we introduce a stochastic model based on interacting particle systems in order to understand the effect of communication on the division of labor. Members of the colony are located on the vertex set of a graph representing a communication network. They are characterized by one of two possible tasks, which they update at a rate equal to the cost of the task they are performing by either defecting by switching to the other task or cooperating by anti-imitating a random neighbor in order to balance the amount of energy spent in each task. We prove that, at least when the probability of defection is small, the division of labor is poor when there is no communication, better when the communication network consists of a complete graph, but optimal on bipartite graphs with bipartite sets of equal size, even when both tasks have very different costs. This shows a non-monotonic relationship between the number of connections in the communication network and how well individuals organize themselves to accomplish both tasks equally.
Fokker–Planck equations in the modeling of socio-economic phenomena
Authors: Furioli Giulia , Pulvirenti Ada , Terraneo Elide , Toscani Giuseppe
Published: November 2016

We present and discuss various one-dimensional linear Fokker–Planck-type equations that have been recently considered in connection with the study of interacting multi-agent systems. In general, these Fokker–Planck equations describe the evolution in time of some probability density of the population of agents, typically the distribution of the personal wealth or of the personal opinion, and are mostly obtained by linear or bilinear kinetic models of Boltzmann type via some limit procedure. The main feature of these equations is the presence of variable diffusion, drift coefficients and boundaries, which introduce new challenging mathematical problems in the study of their long-time behavior.
Continuum dynamics of the intention field under weakly cohesive social interaction
Authors: Degond Pierre , Liu Jian-Guo , Merino-Aceituno Sara , Tardiveau Thomas
Published: November 2016

We investigate the long-time dynamics of an opinion formation model inspired by a work by Borghesi, Bouchaud and Jensen. First, we derive a Fokker–Planck-type equation under the assumption that interactions between individuals produce little consensus of opinion (grazing collision approximation). Second, we study conditions under which the Fokker–Planck equation has non-trivial equilibria and derive the macroscopic limit (corresponding to the long-time dynamics and spatially localized interactions) for the evolution of the mean opinion. Finally, we compare two different types of interaction rates: the original one given in the work of Borghesi, Bouchaud and Jensen (symmetric binary interactions) and one inspired from works by Motsch and Tadmor (non-symmetric binary interactions). We show that the first case leads to a conservative model for the density of the mean opinion whereas the second case leads to a non-conservative equation. We also show that the speed at which consensus is reached asymptotically for these two rates has fairly different density dependence.
Blocks of defect zero in finite groups with conjugate subgroups of a given prime order
Authors: Li Tianze , Liu Yanjun , Qian Guohua
Published: November 2016

Let [Formula: see text] be a finite group and [Formula: see text] be a prime. In this note, we show that if [Formula: see text] and all subgroups of [Formula: see text] of order [Formula: see text] are conjugate, then either [Formula: see text] has a [Formula: see text]-block of defect zero, or [Formula: see text] and [Formula: see text] is a direct product of a simple group [Formula: see text] and an odd order group. This improves one of our previous works.
A note on cosilting modules
Authors: Pop Flaviu
Published: November 2016

The notion of cosilting module was recently introduced as a generalization of the notion of cotilting module. In this paper, we give a characterization of (partial) cosilting modules in terms of two-term cosilting complexes. Moreover, we show that to every cosilting module could be associated a particular [Formula: see text]-structure in the derived module category.
Regularity of weak solutions under pure order differential operators by means of the continuous wavelet transform with rotations
Authors: Navarro Jaime , Elizarraraz David
Published: November 2016

By introducing a rotation parameter besides translations and dilations, the continuous wavelet transform has been defined with respect to an admissible function not necessarily radial to study the local regularity of weak solutions [Formula: see text] under [Formula: see text], where [Formula: see text] is of class [Formula: see text] in a neighborhood of some point [Formula: see text] in [Formula: see text], and [Formula: see text] is a partial differential operator of order [Formula: see text]. The method used to study this fact is through the local convergence of the continuous wavelet transform.
A kinetic model for horizontal transfer and bacterial antibiotic resistance
Authors: Knopoff Damian A. , Sánchez Sansó Juan M.
Published: November 2016

This paper presents a mathematical model for bacterial growth, mutations, horizontal transfer and development of antibiotic resistance. The model is based on the so-called kinetic theory for active particles that is able to capture the main complexity features of the system. Bacterial and immune cells are viewed as active particles whose microscopic state is described by a scalar variable. Particles interact among them and the temporal evolution of the system is described by a generalized distribution function over the microscopic state. The model is derived and tested in a couple of case studies in order to confirm its ability to describe one of the most fundamental problems of modern medicine, namely bacterial resistance to antibiotics.
A Rare Case of Coexistence of Borderline Lepromatous Leprosy with Tuberculosis Verrucosa Cutis
Authors: Dey Biswajit , Gochhait Debasis , Prabhakaran Nagendran , Chandrashekar Laxmisha , Behera Biswanath
Published: November 2016

Occurrence of pulmonary tuberculosis with leprosy is known but association of cutaneous tuberculosis with leprosy is rare. We report a case of borderline lepromatous leprosy coexistent with tuberculosis verrucosa cutis in a 29-year-old male, who presented with multiple skin coloured nodules and hyperkeratotic scaly lesions of 3-month duration. Dual infections are associated with high mortality and morbidity. Therefore early diagnosis and management helps to reduce mortality and to mitigate the effects of morbidity.
Improving Energy Efficiency of Cooperative Femtocell Networks via Base Station Switching Off
Authors: Lee Woongsup , Jung Bang Chul
Published: November 2016

Recently, energy efficiency (EE) of cellular networks has become an important performance metric, and several techniques have been proposed to increase the EE. Among them, turning off base stations (BSs) when not needed is considered as one of the most powerful techniques due to its simple operation and effectiveness. Herein, we propose a novel BS switching-off technique for cooperative femtocell networks where multiple femtocell BSs (FBSs) simultaneously send packets to the same mobile station (MS). Unlike conventional schemes, cooperative operation of FBSs, also known as coordinated multipoint (CoMP) transmission, is considered to determine which BSs are turned off in the proposed technique. We first formulate the optimization problem to find the optimal set of FBSs to be turned off. Then, we propose a suboptimal scheme operating in a distributed manner in order to reduce the computational complexity of the optimal scheme. The suboptimal scheme is based on throughput ratio (TR) which specifies the importance of a particular FBS for the cooperative transmission. Through simulations, we show that the energy consumption can be greatly reduced with the proposed technique, compared with conventional schemes. Moreover, we show that the suboptimal scheme also achieves the near-optimal performance even without the excessive computations.
Fetal Valproate Syndrome with Limb Defects: An Indian Case Report
Authors: Goyal Manisha , Gupta Ashok , Sharma Manish , Mathur Priyanshu , Bansal Naresh
Published: November 2016

Epilepsy is a common disorder and exposure to antiepileptic drugs during pregnancy increases the risk of teratogenicity. Older AEDs such as valproate and phenobarbital are associated with a higher risk of major malformations in the fetus than newer AEDs like lamotrigine and levetiracetam. Exposure to valproic acid during first trimester can result in fetal valproate syndrome (FVS), comprising typical facial features, developmental delay, and a variety of malformations such as neural tube defects, cardiac and genitourinary malformations, and limb defects. We are presenting an Indian case of FVS with major limb defects.
Performance Analysis of the Effect of Nonlinear Low Noise Amplifier for Wideband Spectrum Sensing in the Poisson Field of Interferers
Authors: Man Pati Bipun , Taparugssanagorn Attaphongse
Published: November 2016

A cognitive radio (CR) device likely consists of a low-cost low noise amplifier (LNA) due to the mass-production reason. Nevertheless, the operation of a low-cost LNA becomes highly nonlinear causing intermodulation (IM) interference. The most important task of CR devices is to sense the wideband spectrum to increase opportunistic throughput. In noncooperative secondary networks, the IM interference usually can be ignored for the narrowband spectrum sensing, while the IM interference needs to be taken into account along with interference from other CR devices in the wideband case. Our contribution is to study the effects of a nonlinear LNA for the second case in environments modeled by Poisson field of interferers reflecting more realistic scenario. As shown in the simulation results, the performance of the receiver is degraded in all the cases due to the nonlinearity of LNA. The adaptive threshold setting based on the multivariate Gaussian mixture model is proposed to improve the receiver performance.
Experimental Study of Active Vibration Control of Planar 3-

R

RR Flexible Parallel Robots Mechanism
Authors: Zhang Qinghua , Zhang Xianmin , Wei Junyang
Published: November 2016

An active vibration control experiment of planar 3-RRR flexible parallel robots is implemented in this paper. Considering the direct and inverse piezoelectric effect of PZT material, a general motion equation is established. A strain rate feedback controller is designed based on the established general motion equation. Four control schemes are designed in this experiment: three passive flexible links are controlled at the same time, only passive flexible link 1 is controlled, only passive flexible link 2 is controlled, and only passive flexible link 3 is controlled. The experimental results show that only one flexible link controlled scheme  suppresses elastic vibration and cannot suppress the elastic vibration of the other flexible links, whereas when three passive flexible links are controlled at the same time, they are able to effectively suppress the elastic vibration of all of the flexible links. In general, the experiment verifies that a strain rate feedback controller is able to effectively suppress the elastic vibration of the flexible links of plane 3-RRR flexible parallel robots.
Thymic Epidermoid Cyst: Clinical and Imaging Manifestations of This Rare Anterior Mediastinal Mass
Authors: Qureshi Jawad M. , Pagano Brian , Mueller Jeffrey , Schumacher Lana , Velosa Claudia , Hartman Matthew S.
Published: November 2016

Thymic epidermoid cysts are an extremely rare entity. These arise from epidermal cells that migrate to the thymus. The radiologic diagnosis of this rare lesion is challenging. We describe a case of an otherwise healthy 35-year-old woman who presented with an acute onset of chest pain and shortness of breath. She was found to have an anterior mediastinal mass. The imaging findings were, however, not characteristic for any single diagnostic entity. Since the imaging was inconclusive, surgical resection was performed for definitive diagnosis. The mass was found to be a thymic epidermoid cyst. This case underlines the significance for radiologists to be aware that epidermoid cysts can occur in the thymus and should be considered in the differential diagnosis for a heterogeneous anterior mediastinal mass.
Sound Radiation Characteristics of a Rectangular Duct with Flexible Walls
Authors: Raviprolu Praveena , Jade Nagaraja , Balide Venkatesham
Published: November 2016

Acoustic breakout noise is predominant in flexible rectangular ducts. The study of the sound radiated from the thin flexible rectangular duct walls helps in understanding breakout noise. The current paper describes an analytical model, to predict the sound radiation characteristics like total radiated sound power level, modal radiation efficiency, and directivity of the radiated sound from the duct walls. The analytical model is developed based on an equivalent plate model of the rectangular duct. This model has considered the coupled and uncoupled behaviour of both acoustic and structural subsystems. The proposed analytical model results are validated using finite element method (FEM) and boundary element method (BEM). Duct acoustic and structural modes are analysed to understand the sound radiation behaviour of a duct and its equivalence with monopole and dipole sources. The most efficient radiating modes are identified by vibration displacement of the duct walls and for these the radiation efficiencies have been calculated. The calculated modal radiation efficiencies of a duct compared to a simple rectangular plate indicate similar radiation characteristics.
Scavenger Receptor Class B, Type I, a CD36 Related Protein inMacrobrachium nipponense: Characterization, RNA Interference, and Expression Analysis with Different Dietary Lipid Sources
Authors: Ding Zhili , Luo Na , Kong Youqin , Li Jingfen , Zhang Yixiang , Cao Fang , Ye Jinyun
Published: November 2016

The scavenger receptor class B, type I (SR-BI), is a member of the CD36 superfamily comprising transmembrane proteins involved in mammalian and fish lipid homeostasis regulation. We hypothesize that this receptor plays an important role inMacrobrachium nipponenselipid metabolism. However, little attention has been paid to SR-BI in commercial crustaceans. In the present study, we report a cDNA encodingM. nipponensescavenger receptor class B, type I (designated as MnSR-BI), obtained from a hepatopancreas cDNA library. The complete MnSR-BI coding sequence was 1545 bp, encoding 514 amino acid peptides. The MnSR-BI primary structure consisted of a CD36 domain that contained two transmembrane regions at the N- and C-terminals of the protein. SR-BI mRNA expression was specifically detected in muscle, gill, ovum, intestine, hepatopancreas, stomach, and ovary tissues. Furthermore, its expression in the hepatopancreas was regulated by dietary lipid sources, with prawns fed soybean and linseed oils exhibiting higher expression levels. RNAi-based SR-BI silencing resulted in the suppression of its expression in the hepatopancreas and variation in the expression of lipid metabolism-related genes. This is the first report of SR-BI in freshwater prawns and provides the basis for further studies on SR-BI in crustaceans.
Changes in Serum Concentrations of Fibroblast Growth Factor 23 and Soluble Klotho in Hemodialysis Patients after Total Parathyroidectomy
Authors: Liao Shang-Chih , Moi Sin-Hua , Chou Fong-Fu , Yang Cheng-Hong , Chen Jin-Bor
Published: November 2016

Background. We examined the changes in circulating fibroblast growth factor 23 (FGF23) and Klotho concentrations in hemodialysis patients after parathyroidectomy (PTX). Methods. We enrolled a cohort of hemodialysis patients who received PTX. Postoperatively, patients received calcium supplements and/or vitamin D analogue (calcitriol) to maintain serum calcium within 7.0–8.0 mg/dL. Information on clinical parameters including bone-mineral metabolic variables was collected pre-PTX and on days 5 and 90 after PTX. Concomitantly, serum full-length FGF23 and α-Klotho levels were measured. The relationship between FGF23 and clinical parameters was analyzed by single linear regression. Results. Forty-six participants (33 women; 13 men) were enrolled in the study. Their mean age was 56.49 years. Serum FGF23 and α-Klotho concentrations were elevated on days 5 and 90 after PTX compared to baseline (p>0.05). Serum FGF23 concentrations negatively correlated with serum calcium concentrations pre-PTX (Beta -0.31; R2 0.0949; p=0.040), day 5 post-PTX (Beta -0.31; R2 0.0982; p=0.036), and day 90 post-PTX (Beta -0.39; R2 0.1528; p=0.008). Conclusions. There was no change in circulating FGF23 and Klotho concentrations after PTX in hemodialysis patients given postoperative calcium supplements and/or vitamin D analogue. Serum FGF23 concentrations pre-PTX and at days 5 and 90 after PTX were inversely related to serum calcium concentrations.
Peripheral Giant Cell Granuloma in a Child Associated with Ectopic Eruption and Traumatic Habit with Control of Four Years
Authors: Volpato Luiz Evaristo Ricci , Leite Cristhiane Almeida , Anhesini Brunna Haddad , Aguilera Jéssica Marques Gomes da Silva , Borges Álvaro Henrique
Published: November 2016

Peripheral giant cell granuloma (PGCG) is a nonneoplastic lesion that may affect any region of the gingiva or alveolar mucosa of edentulous and toothed areas, preferentially in the mandible and rarely occurring in children. This report describes the clinical and histopathological findings of a PGCG diagnosed in the maxilla of a 9-year-old boy associated with a tooth erupting improperly and a traumatic habit. The patient did not present anything noteworthy on extraoral physical examination or medical history, but the habit of picking his teeth and “poking” the gingiva. The oral lesion consisted of an asymptomatic, rounded, pink colored, smooth surface, soft tissue injury with fibrous consistency and approximated size of 1.5 cm located in the attached gingiva between the upper left permanent lateral incisor and the primary canine of the same side. Excisional biopsy was performed through curettage and removal of the periosteum, periodontal ligament, and curettage of the involved teeth with vestibular access. The histopathological analysis led to the diagnosis of PGCG. The prompt diagnosis and treatment of the PGCG resulted in a more conservative surgery and a reduced risk for tooth and bone loss and recurrence of the lesion. After four years of control, patient had no relapse of the lesion and good gingival and osseous health.
Genetic Diversity of Cowpea (Vigna unguiculata (L.) Walp.) Accession in Kenya Gene Bank Based on Simple Sequence Repeat Markers
Authors: Wamalwa Emily N. , Muoma John , Wekesa Clabe
Published: November 2016

Increased agricultural production is an urgent issue. Projected global population is 9 million people by mid of this century. Estimation projects death of 1 million people for lack of food quality (micronutrient deficit) and quantity (protein deficit). Majority of these people will be living in developing countries. Other global challenges include shrinking cultivable lands, salinity, and flooding due to climate changes, new emerging pathogens, and pests. These affect crop production. Furthermore, they are major threats to crop genetic resources and food security. Genetic diversity in cultivated crops indicates gene pool richness. It is the greatest resource for plant breeders to select lines that enhance food security. This study was conducted by Masinde Muliro University to evaluate genetic diversity in 19 cowpea accessions from Kenya national gene bank. Accessions clustered into two major groups. High divergence was observed between accessions from Ethiopia and Australia and those from Western Kenya. Upper Volta accessions were closely related to those from Western Kenya. Low variation was observed between accessions from Eastern and Rift Valley than those from Western and Coastal regions of Kenya. Diversity obtained in this study can further be exploited for the improvement of cowpea in Kenya as a measure of food security.
Disruption of Autophagic Degradation with ROC-325 Antagonizes Renal Cell Carcinoma Pathogenesis
Authors: Carew Jennifer S. , Espitia Claudia M. , Zhao William , Han Yingchun , Visconte Valeria , Phillips James , Nawrocki Steffan T.
Published: November 2016

Purpose: Although autophagy plays important roles in malignant pathogenesis and drug resistance, there are few clinical agents that disrupt this pathway, and the potential therapeutic benefit of autophagy inhibition remains undetermined. We used medicinal chemistry approaches to generate a series of novel agents that inhibit autophagic degradation.

Experimental Design: ROC-325 was selected as a lead compound for further evaluation. Comprehensive in vitro and in vivo studies were conducted to evaluate the selectivity, tolerability, and efficacy of ROC-325 in preclinical models of renal cell carcinoma (RCC) with HCQ serving as a comparator. Markers of autophagy inhibition and cell death were evaluated in tumor specimens.

Results: ROC-325 exhibited superior in vitro anticancer effects compared with the existing autophagy inhibitor hydroxychloroquine (HCQ) in 12 different cancer cell lines with diverse genetic backgrounds. Focused studies of the mechanism of action and efficacy of ROC-325 in RCC cells showed that drug treatment induced hallmark characteristics of autophagy inhibition, including accumulation of autophagosomes with undegraded cargo, lysosomal deacidification, p62 stabilization, and disruption of autophagic flux. Subsequent experiments showed that ROC-325 antagonized RCC growth and survival in an ATG5/7-dependent manner, induced apoptosis, and exhibited favorable selectivity. Oral administration of ROC-325 to mice bearing 786-0 RCC xenografts was well tolerated, was significantly more effective at inhibiting tumor progression than HCQ, and inhibited autophagy in vivo.

Conclusions: Our findings demonstrate that ROC-325 has superior preclinical anticancer activity compared with HCQ and support the clinical investigation of its safety and preliminary efficacy in patients with RCC and other autophagy-dependent malignancies. Clin Cancer Res; 23(11); 2869–79. ©2016 AACR.
An miRNA–DNMT1 Axis Is Involved in Azacitidine Resistance and Predicts Survival in Higher-Risk Myelodysplastic Syndrome and Low Blast Count Acute Myeloid Leukemia
Authors: Solly Françoise , Koering Catherine , Mohamed Aminetou Mint , Maucort-Boulch Delphine , Robert Guillaume , Auberger Patrick , Flandrin-Gresta Pascale , Adès Lionel , Fenaux Pierre , Kosmider Olivier , Tavernier-Tardy Emmanuelle , Cornillon Jérôme , Guyotat Denis , Campos Lydia , Mortreux Franck , Wattel Eric
Published: November 2016

Purpose: Azacitidine inhibits DNA methyltransferases, including DNMT1, and is currently the standard of care for patients with higher-risk myelodysplastic syndrome (HRMDS) or low blast count acute myeloid leukemia (AML).

Experimental Design: The expression of 754 miRNAs was compared in azacitidine-resistant and azacitidine-sensitive myelodysplastic syndrome cells. We investigated the role of differentially expressed miRNAs on DNMT1 expression and azacitidine resistance in vitro. We next evaluated anti-DNMT1 miRNA expression in pretreatment bone marrow samples derived from 75 patients treated with azacitidine for HRMDS or AML.

Results: Seven miRNAs, including 5 that in silico targeted the DNMT1 3′ UTR, were repressed in azacitidine-resistant cells in which DNMT1 protein levels were significantly higher. Ectopic anti-DNMT1 miRNA expression decreased DNMT1 expression and increased azacitidine sensitivity, whereas specific inhibition of endogenous anti-DNMT1 miRNAs increased DNMT1 expression and triggered azacitidine resistance. In patients treated with azacitidine, decreased expression of anti-DNMT1 miRNAs was associated with poor outcome. miR-126* had the strongest prognostic impact. Patients with miR-126*low myelodysplastic syndrome had significantly lower response rates (P = 0.04) and higher relapse rates (P = 0.03), as well as shorter progression-free (PFS; P = 0.004) and overall survival (OS; P = 0.004). Multivariate analysis showed that age, miR-126* expression, and revised International Prognostic Scoring System risk independently predicted PFS and OS. In 15 patient samples collected over time, decreased miRNA expression levels were associated with secondary resistance.

Conclusions: A decreased expression of anti-DNMT1 miRNAs might account for azacitidine resistance in HRMDS and AML, and measuring miRNA expression before and during treatment might help predict primary or secondary azacitidine resistance. Clin Cancer Res; 23(12); 3025–34. ©2016 AACR.
Telencephalic Neuronal Activation Associated with Spatial Memory in the Terrestrial Toad Rhinella arenarum: Participation of the Medial Pallium during Navigation by Geometry
Authors: Sotelo María Inés , Daneri M. Florencia , Bingman Verner Peter , Muzio Rubén N.
Published: November 2016

Amphibians are central to discussions of vertebrate evolution because they represent the transition from aquatic to terrestrial life, a transition with profound consequences for the selective pressures shaping brain evolution. Spatial navigation is one class of behavior that has attracted the interest of comparative neurobiologists because of the relevance of the medial pallium/hippocampus, yet, surprisingly, in this regard amphibians have been sparsely investigated. In the current study, we trained toads to locate a water goal relying on the boundary geometry of a test environment (Geometry-Only) or boundary geometry coupled with a prominent, visual feature cue (Geometry-Feature). Once learning had been achieved, the animals were given one last training session and their telencephali were processed for c-Fos activation. Compared to control toads exposed to the test environment for the first time, geometry-only toads were found to have increased neuronal labeling in the medial pallium, the presumptive hippocampal homologue, while geometry-feature toads were found to have increased neuronal labeling in the medial, dorsal, and lateral pallia. The data indicate medial pallial participation in guiding navigation by environmental geometry and lateral, and to a lesser extent dorsal, pallial participation in guiding navigation by a prominent visual feature. As such, participation of the medial pallium/hippocampus in spatial cognition appears to be a conserved feature of terrestrial vertebrates even if their life history is still tied to water, a brain-behavior feature seemingly at least as ancient as the evolutionary transition to life on land.
Multiple Magnetic Foreign Bodies Causing Severe Digestive Tract Injuries in a Child
Authors: Si Xinmin , Du Baofeng , Huang Lei
Published: November 2016

Foreign body (FB) ingestion is a common emergency as well as a major cause of accidental injury and represents a severe public health problem in childhood, especially in infants. Most cases of FB ingestion reported in children aged between 6 months and 3 years depend primarily on the fact that young children are more likely to explore objects using their mouth and are not able to distinguish edible objects from nonedible ones, their teeth are physiologically lacking, and they have poor swallowing coordination. Although, sometimes it can cause serious complications, FB ingestion generally has a low mortality rate. However, accidental ingestion of magnetic toys, as a rare kind of FB mostly encountered in children, has now become more common due to the increased availability of objects and toys with magnetic elements. The majority of magnetic FB traverse the gastrointestinal (GI) system spontaneously without complication, but in rare cases may cause severe damages to the GI tract due to its special pathogenesis [Kay and Wyllie: Curr Gastroenterol Rep 2005;7: 212–218]. Ingestion of multiple magnets may be related to increased morbidity resulting in a delay of recognition of FB injury that can lead to serious complications and require surgical resolution.
Dietary and Alcohol Intake and Central Nervous System Tumors in Adults: Results of the CERENAT Multicenter Case-Control Study
Authors: Allès Benjamin , Pouchieu Camille , Gruber Anne , Lebailly Pierre , Loiseau Hugues , Fabbro-Peray Pascale , Letenneur Luc , Baldi Isabelle
Published: November 2016

Background: Little is known about the relationship between diet and central nervous system (CNS) tumors, especially in terms of their histological subtypes. This study investigated the overall associations between food groups, alcohol intake and CNS tumors, and in particular about the associations between neuroepithelial tumors and meningiomas. Methods: Data were collected through the CERENAT (CEREbral tumors: a NATional study) case-control study conducted in France during the period 2004-2010. Data were available for 1,479 subjects (494 cases, including 201 neuroepithelial tumors, 193 meningiomas, 100 other CNS tumors, and their 985 matched controls). Conditional logistic regressions for matched sets were adjusted based on the participants' educational level, occupation, smoking status and frequency of food group consumption. Results: A heavy consumption of grilled meat and poultry was associated with neuroepithelial tumors in a dose-related relationship (ORQ4vsQ1 = 3.72, 95% CI 1.62-8.52, p = 0.005). Higher fruit and vegetable intake was inversely associated with meningiomas (for fruits: ORQ4vsQ1 = 0.38, 95% CI 0.17-0.87, p = 0.06, for vegetables ORQ4vsQ1 = 0.26, 95% CI 0.11-0.62, p = 0.007). Consumption of alcohol on a daily basis was inversely associated with CNS tumors especially for meningiomas (ORQ4vsQ1 = 0.33, 95% CI 0.18-0.61, p = 0.001). Conclusions: Results obtained in terms of grilled meat, fruits and vegetables consumption were in line with those published in epidemiological literature. Contradictions in results between neuroepithelial tumors and meningiomas confirmed the need to analyze the effects of dietary factors on the basis of the histological subtypes of CNS tumors.
Immunohistochemical Heterogeneity of the Endothelium of Blood and Lymphatic Vessels in the Developing Human Liver and in Adulthood
Authors: Nikolić Ivan , Todorović Vera , Petrović Aleksandar , Petrović Vladimir , Jović Marko , Vladičić Jelena , Puškaš Nela
Published: November 2016

The endothelium of liver sinusoids in relation to the endothelium of other blood vessels has specific antigen expression similar to the endothelium of lymphatic vessels. Bearing in mind that there is no consensus as to the period or intensity of the expression of certain antigens in the endothelium of blood and lymphatic vessels in the liver, the aim of our study was to immunohistochemically investigate the dynamic patterns of the expression of CD31, CD34, D2-40, and LYVE-1 antigens during liver development and in adulthood on paraffin tissue sections of human livers of 4 embryos, 38 fetuses, 6 neonates, and 6 adults. The results show that, in a histologically immature liver at the end of the embryonic period, CD34 molecules are expressed only on vein endothelium localized in developing portal areas, whereby the difference between portal venous branches and CD34-negative central veins belongs to the collecting venous system. In the fetal period, with aging, expression of CD34 and CD31 molecules on the endothelium of central veins and blood vessels of the portal areas increases. Sinusoidal endothelium shows light and sporadic CD34 immunoreactivity in the late embryonic and fetal periods, and is lost in the neonatal and adult periods, unlike CD31 immunoreactivity, which is poorly expressed in the fetal and neonatal periods but is present in adults. The endothelium of sinusoids and lymphatic vessels express LYVE-1, and the endothelium of lymphatic vessels express LYVE-1 and D2-40 but not CD34. Similarity between the sinusoidal and lymphatic endothelium includes the fact that both types are LYVE-1 positive and CD34 negative.
Sodium Pentosan Polysulfate Reduced Renal Ischemia-Reperfusion-Induced Oxidative Stress and Inflammatory Responses in an Experimental Animal Model
Authors: Hardi Péter , Nagy Tibor , Fazekas Gábor , Arató Endre , Menyhei Gábor , Sétáló Jr. György , Vecsernyés Mónika , Pintér Örs , Takács Ildikó , Bohonyi Noémi , Jancsó Gábor
Published: November 2016

Acute kidney injury (AKI) remains an independent risk factor for mortality and morbidity after vascular surgery (affecting the renal arteries) or aortic surgery (requiring suprarenal aortic clamping). These types of vascular surgery produce renal ischemia/reperfusion (I/R) injury, a common cause of AKI. The present studies aimed at monitoring the course of renal I/R injury at the cellular level and investigating the efficacy of long-term preoperative and single-shot intraoperative administration of sodium pentosan polysulfate (PPS) to protect renal tissue from acute I/R injury both in native and diabetic kidneys in rats. Western blot analyses of the proapoptotic (bax) and antiapoptotic (bcl-2) signaling pathways, as well as the extent of DNA damage (phospho-p53), were performed. Oxidative stress followed upon the termination of malondialdehyde, reduced glutathione, thiol group, and superoxide dismutase plasma levels. Inflammatory changes were measured by the determination of serum tumor necrosis factor-α and interleukin-1 levels. Morphological changes were detected by histological examinations. Our results showed that the long-term administration of PPS has an advantage in reducing I/R kidney injury in diabetic rats, while high-dose, single-shot parenteral administration of PPS prior to revascularization might be useful in nondiabetic rats.
Usefulness of Discriminability and Response Bias Indices for the Evaluation of Recognition Memory in Mild Cognitive Impairment and Alzheimer Disease
Authors: Russo María Julieta , Cohen Gabriela , Campos Jorge , Martin Maria Eugenia , Clarens María Florencia , Sabe Liliana , Barcelo Ernesto , Allegri Ricardo F.
Published: November 2016

Background: Most studies examining episodic memory in Alzheimer disease (AD) have focused on patients' impaired ability to remember information. This approach provides only a partial picture of memory deficits since other factors involved are not considered. Objective: To evaluate the recognition memory performance by using a yes/no procedure to examine the effect of discriminability and response bias measures in amnestic mild cognitive impairment (a-MCI), AD dementia, and normal-aging subjects. Methods: We included 43 controls and 45 a-MCI and 51 mild AD dementia patients. Based on the proportions of correct responses (hits) and false alarms from the Rey Auditory Verbal Learning Test (RAVLT), discriminability (d′) and response bias (C) indices from signal detection theory (SDT) were calculated. Results: Results showed significant group differences for d′ (F (2) = 83.26, p < 0.001), and C (F (2) = 6.05, p = 0.00). The best predictors of group membership were delayed recall and d′ scores. The d′ measure correctly classified subjects with 82.98% sensitivity and 91.11% specificity. Conclusions: a-MCI and AD dementia subjects exhibit less discrimination accuracy and more liberal response bias than controls. Furthermore, combined indices of delayed recall and discriminability from the RAVLT are effective in defining early AD. SDT may help enhance diagnostic specificity.
Pamidronate Attenuates Oxidative Stress and Energetic Metabolism Changes but Worsens Functional Outcomes in Acute Doxorubicin-Induced Cardiotoxicity in Rats
Authors: Carvalho Paula Bernardo de , Gonçalves Andréa de Freitas , Alegre Patrícia Helena Correa , Azevedo Paula Schmidt , Roscani Meliza Goi , Bergamasco Carolina Marabesi , Modesto Pamela N. , Fernandes Ana Angélica , Minicucci Marcos Ferreira , Paiva Sergio Alberto Rupp , Antonio Leonardo , Zornoff Mamede , Polegato Bertha Furlan
Published: November 2016

Background: Cardiotoxicity is the major side effect of doxorubicin. As mechanisms that are involved in cardiotoxicity are ambiguous, new methods for attenuating cardiotoxicity are needed. Recent studies have shown that bisphosphonates can decrease oxidative stress. Therefore, the objective of this study was to evaluate the effect of pamidronate on preventing acute doxorubicin-induced cardiotoxicity. Methods: Sixty-four male Wistar rats were allocated into four groups: the control group (C), the pamidronate group (P), the doxorubicin group (D) and the doxorubicin/pamidronate group (DP). The rats in the P and DP groups received pamidronate injections (3 mg/kg, IP). After 24 hours, the rats in the D and DP groups received doxorubicin injections (20 mg/kg, IP). Forty-eight hours after doxorubicin injection, the rats were killed. Echocardiography, isolated heart study and biochemical analysis were performed. Results: Doxorubicin-induced acute cardiotoxicity showed increased matrix metalloproteinases (MMP)-2 activation, oxidative damage and induced alterations in myocardial energetic metabolism. Pamidronate did not inhibit MMP-2 activation but attenuated oxidative stress and improved myocardial energetic metabolism. Regarding cardiac function, the DP group exhibited a decrease in the left ventricular ejection fraction in the echocardiography and a decrease in +dP/dt in the isolated heart study compared with other groups. The same DP group presented serum hypocalcaemia. Conclusions: Despite its ability to reduce oxidative stress and improve energy metabolism in the heart, pamidronate worsened systolic function in rats treated with doxorubicin, and therefore we cannot recommend its use in conjunction with anthracycline chemotherapy.
Cilostazol Suppresses IL-23 Production in Human Dendritic Cells via an AMPK-Dependent Pathway
Authors: Shi Quanxing , Yin Zhao , Liu Peilin , Zhao Bei , Zhang Zhong , Mao Shuai , Wei Tengfei , Rao Mengmeng , Zhang Liguo , Wang Shouli
Published: November 2016

Background/Aims: Cilostazol has been previously demonstrated to inhibit IL-23 production in human synovial macrophages via a RhoA/ROCK-dependent pathway. However, whether cilostazol affects IL-23 production in human dendritic cells remains largely unknown. The present study was designed to investigate this question and elucidate the possible underlying mechanisms. Methods: Human monocyte-derived dendritic cells (mo-DCs) were pretreated with or without cilostazol and then incubated with zymosan. Enzyme-linked immunosorbent assay (ELISA) and real time PCR analyses were used to measure IL-23 protein expression and RNA levels, respectively, whereas Western blotting was used to measure the expression and phosphorylation level of AMPK. Results: Our results demonstrated that cilostazol suppressed zymosan-induced IL-23 protein production in a concentration dependent manner without affecting dendritic cell viability. In addition, it was found that cilostazol suppressed the expression of the p19 and p40 subunits of IL-23. Moreover, cilostazol mimicked the effect of the AMPK agonist A-769662, as demonstrated by the fact that IL-23 production was also inhibited by A-769662, and the effect of cilostazol on IL-23 production was blocked by the AMPK antagonist Compound C. More importantly, Western blotting demonstrated that cilostazol led to an increased phosphorylation of AMPK. Conclusion: Collectively, our data suggest that cilostazol inhibits the production of IL-23 in human mo-DCs, potentially via the activation of AMPK. This suggests that cilostazol could be an effective anti-inflammatory agent in IL-23- and dendritic cell-related diseases.
Evaluation of KIM-1 and NGAL as Early Indicators for Assessment of Gentamycin-Induced Nephrotoxicity In Vivo and In Vitro
Authors: Luo Qi-Hui , Chen Meng-Lu , Chen Zheng-Li , Huang Chao , Cheng An-Chun , Fang Jing , Tang Li , Geng Yi
Published: November 2016

Background/Aims: The aminolycoside Gentamicin is a widely used antibiotic, applied in equine medicine. Despite its clinical use, concerns remain regarding the potential toxic side-effects, such as nephrotoxicity. Early detection of renal damage is critical in preclinical drug development. This study was aimed to determine whether kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) may be early indicators in the assessment of Gentamycin-induced nephrotoxicity. Methods: In our study, a model of Gentamicin-induced nephrotoxicity in male Sprague Dawley rats treated for up to 7 days at 50 or 100mg/kg/day was used to monitor the expressions of novel biomarkers of renal toxicity during the progression of acute kidney injury (AKI). Additionally, biomarkers were assessed in human kidney proximal epithelial cells (HK-2) treated with Gentamicin for 2, 6, 12, 24, 36 or 48h in vitro. Results: Repeated administration of Gentamicin to rats for 1, 3, or 7 days resulted in a dose- and time-dependent increase in the expression of KIM-1 and NGAL. The expressions of the two biomarkers changed prior to renal tubule damage and increases in serum creatinine (SCr) and blood urea nitrogen (BUN) levels, suggesting their usefulness for predicting Gentamicin-induced acute kidney injury (AKI) in vivo. Conclusions: In contrast, no significant increase in the expression of the biomarker genes and proteins were evident in HK-2 cells after treated by Gentamycin for up to 48h, suggesting that they may not be suitable endpoints for sensitive detection of nephrotoxic effects in vitro.
Clinical Utility of Serum Cystatin C in Predicting Diabetic Nephropathy Among Patients with Diabetes Mellitus: a Meta-Analysis
Authors: Zhou Baoqin , Zou Honghong , Xu Gaosi
Published: November 2016

Background/Aims: Clinically, there is lack of predictors for diabetic nephropathy (DN) in diabetes mellitus (DM) without microalbuminuria, macroalbuminuria or retinopathy. Methods: PubMed, Chinese Biomedical Database, Cochrane Library, EMBASE and Elsevier Database were searched from inception to August 13, 2016. Studies involving patients with DM and containing data on cystatin C measurements and the measured glomerular filtration rate (mGFR) were included. Pooled sensitivity, specificity, positive predictive value, negative predictive value and other diagnostic indices were evaluated using a random effect model. Results: The meta-analysis enrolled 9 studies with 1417 patients. The pooled sensitivity and specificity of serum cystatin C for predicting DN were 0.88 (95% CI 0.85 - 0.91) and 0.85 (95% CI 0.82 - 0.87), respectively. The pooled positive and negative predictive values of serum cystatin C for predicting DN were 7.04 (95% CI 4.33 - 11.43) and 0.13 (95% CI 0.09 - 0.20), respectively. The area under the summary receiver operating characteristic (SROC) curve was 0.9549, and the diagnostic odds ratio was 66.80 (95% CI 27.92 - 159.86). Conclusion: Serum cystatin C is an early predictor of DN among patients with DM.