bluuebunny/crossref_metadata_2025 huggingface dataset:
Magnetic Analysis of MgFe Hydrotalcites as Powder and Dispersed in Thin Films within a Keratin Matrix
Authors: Dinelli Franco , Modestino Michele , Galluzzi Armando , Posati Tamara , Seri Mirko , Zamboni Roberto , Sotgiu Giovanna , Polichetti Massimiliano
Published: July 2023

Hydrotalcites (HTlcs) are a class of nanostructured layered materials that may be employed in a variety of applications, from green to bio technologies. In this paper, we report an investigation on HTlcs made of Mg and Fe, recently employed to improve the growth in vitro of osteoblasts within a keratin sponge. We carried out an analysis of powder materials and of HTlcs dispersed in keratin and spin-coated on a Si/SiO2 substrate at different temperatures. A magnetic study of the powders was carried out with a Quantum Design Physical Property Measurement System equipped with a Vibrating Sample Magnetometer. The data gathered prove that these HTlcs are fully paramagnetic, and keratin showed a very small magnetic response. Optical and Atomic Force Microscopy analyses of the thin films provide a detailed picture of clusters randomly dispersed in the films with various dimensions. The magnetic properties of these films were characterized using the Nano Magneto Optical Kerr Effect (NanoMOKE) down to 7.5 K. The data collected show that the local magnetic properties can be mapped with a micrometric resolution distinguishing HTlc regions from keratin ones. This approach opens new perspectives in the characterization of these composite materials.
Coffee Consumption and Risk of Hypertension in Adults: Systematic Review and Meta-Analysis
Authors: Haghighatdoost Fahimeh , Hajihashemi Parisa , de Sousa Romeiro Amanda Maria , Mohammadifard Noushin , Sarrafzadegan Nizal , de Oliveira Cesar , Silveira Erika Aparecida
Published: July 2023

Objectives: The association between coffee intake and hypertension (HTN) risk is controversial. Therefore, this systematic review and meta-analysis aimed at summarizing the current evidence on the association of coffee with hypertension risk in observational studies. Methods: PubMed/Medline and Web of Science were searched for observational studies up to February 2023. Observational studies which assessed the risk of HTN in the highest category of coffee consumption in comparison with the lowest intake were included in the current meta-analysis (registration number: CRD42022371494). The pooled effect of coffee on HTN was evaluated using a random-effects model. Results: Twenty-five studies i.e., thirteen cross-sectional studies and twelve cohorts were identified to be eligible. Combining 13 extracted effect sizes from cohort studies showed that higher coffee consumption was associated with 7% reduction in the risk of HTN (95% CI: 0.88, 0.97; I2: 22.3%), whereas combining 16 effect sizes from cross-sectional studies illustrated a greater reduction in HTN risk (RR = 0.79, 95% CI: 0.72, 0.87; I2 = 63.2%). These results varied by studies characteristics, such as the region of study, participants’ sex, study quality, and sample size. Conclusions: An inverse association was found between coffee consumption and hypertension risk in both cross-sectional and cohort studies. However, this association was dependent on studies characteristics. Further studies considering such factors are required to confirm the results of this study.
Impact of Lung-Related Polygenic Risk Scores on Chronic Obstructive Pulmonary Disease Risk and Their Interaction with w-3 Fatty Acid Intake in Middle-Aged and Elderly Individuals
Authors: Kim Ki-Song , Park Sunmin
Published: July 2023

Chronic obstructive pulmonary disease (COPD) is a complex, progressive respiratory disorder with persistent airflow limitation and tissue destruction. We aimed to explore the genetic impact of COPD and its interaction with nutrient intake in 8840 middle-aged and elderly individuals from the Ansan/Ansung cohorts. Participants were diagnosed with COPD if the ratio of forced expiratory volume in 1 s (FEV1) to forced vital capacity (FVC) was less than 0.7 using spirometry, and if they were previously diagnosed with COPD by a physician. Genome-wide association studies (GWAS) were performed to screen for genetic variants associated with COPD risk. Among them, we selected the genetic variants that exhibited interactions using the generalized multifactor dimensionality reduction (GMDR) method. The polygenic risk score (PRS) was computed by summing the number of risk alleles in the SNP-SNP interaction models that adhered to specific rules. Subsequently, participants were categorized into low-PRS, medium-PRS, and high-PRS groups. The participants with COPD exhibited significantly lower FEV1/FVC ratios (0.64) than those without COPD (0.82). It was positively associated with inflammation markers (serum C-reactive protein and white blood cell levels). A higher proportion of COPD participants were smokers and engaged in regular exercise. The 5-SNP model consisted of FAM13A_rs1585258, CAV1_rs1997571, CPD_rs719601, PEPD_rs10405598, and ITGA1_rs889294, and showed a significant association with COPD risk (p < 0.001). Participants in the high-PRS group of this model had a 2.2-fold higher risk of COPD than those in the low-PRS group after adjusting for covariates. The PRS interacted with w-3 fatty acid intake and exercise, thus influencing the risk of COPD. There was an increase in COPD incidence among individuals with a higher PRS, particularly those with low consumption of w-3 fatty acid and engaged in high levels of exercise. In conclusion, adults with a high-PRS are susceptible to COPD risk, and w-3 fatty acid intake and exercise may impact the risk of developing COPD, potentially applying to formulate precision medicines to prevent COPD.
Large Iodine Variability in Retail Cows’ Milk in the U.S.: A Follow-Up Study among Different Retail Outlets
Authors: Roseland Janet M. , Phillips Katherine M. , Vinyard Bryan T. , Todorov Todor , Ershow Abby G. , Pehrsson Pamela R.
Published: July 2023

In a previous study, large variability in iodine content was found among samples of store brand retail milk at a single time point in a sampling taken from 24 nationwide U.S. locations for the USDA FoodData Central database, but the sampling plan was not designed to detect differences among locations. This follow-up study was carried out to evaluate iodine levels in retail milk across the U.S. over time. Milk samples (2% fat) were collected bimonthly in fourteen locations for one year and analyzed in duplicate. Control materials were used to support accuracy of results and ensure precision across analytical batches. The overall mean and standard error (SE) for iodine concentration were 82.5 (7.0) µg/240 mL serving, which was comparable to the previous national mean [85.0 (5.5) µg/240 mL]. A similar wide range among individual samples was detected (27.9–282 µg/240 mL). For some locations, the mean iodine concentration differed significantly from others, and differed from the national average by amounts ranging from −47 µg to +37 µg per serving. The between-sample range within location was large for some (up to 229 µg/serving) and minimal for others (as little as 13.2 µg/serving). These findings suggest iodine intake from some retail milk supplies could be over- or underestimated relative to the national average, even if the national average is suitable for population-wide intake estimates.
Meat Consumption and Availability for Its Reduction by Health and Environmental Concerns: A Pilot Study
Authors: Turnes Andrea , Pereira Paula , Cid Helena , Valente Ana
Published: July 2023

(1) Background: Excessive meat consumption has raised multiple health and environmental concerns; however, there are no data on the population’s willingness to reduce its intake for these reasons. The current study aims to assess the frequency of meat intake and readiness to limit consumption due to concern about the impact on health and the environment in residents of the Lisbon metropolitan region. (2) Methods: This analytical cross-sectional observational study was carried out in 197 residents in the metropolitan region of Lisbon. The participants were divided into two groups by age (GI: 20–29 years; GII: 40–64 years). Meat consumption and willingness to reduce it were assessed through a questionnaire. (3) Results: Most participants (67%) reported not having knowledge about the ecological footprint of meat. Being a less frequent meat consumer (<1 time per day) is associated with a willingness 3.6 times higher (p < 0.001) to reduce meat consumption due to sensitivity to the impact on health and 4.0 times higher (p < 0.001) due to environmental reasons. (4) Conclusions: Lower meat consumption frequency was associated with reductions in this consumption for environmental and health reasons.
Personalized Nutritional Strategies to Reduce Knee Osteoarthritis Severity and Ameliorate Sarcopenic Obesity Indices: A Practical Guide in an Orthopedic Setting
Authors: Zmerly Hassan , El Ghoch Marwan , Itani Leila , Kreidieh Dima , Yumuk Volkan , Pellegrini Massimo
Published: July 2023

Knee osteoarthritis (KOA) is one of the most common joint diseases, especially in individuals with obesity. Another condition within this population, and which presents frequently, is sarcopenic obesity (SO), defined as an increase in body fat and a decrease in muscle mass and strength. The current paper aims to describe recent nutritional strategies which can generally improve KOA clinical severity and, at the same time, ameliorate SO indices. Searches were carried out in the PubMed and Science Direct databases and data were summarized using a narrative approach. Certain key findings have been revealed. Firstly, the screening and identification of SO in patients with KOA is important, and to this end, simple physical performance tests and anthropometric measures are available in the literature. Secondly, adherence to a Mediterranean diet and the achievement of significant body weight loss by means of low-calorie diets (LCDs) remain the cornerstone nutritional treatment in this population. Thirdly, supplementation with certain micronutrients such as vitamin D, essential and non-essential amino acids, as well as whey protein, also appear to be beneficial. In conclusion, in the current review, we presented a detailed flowchart of three different nutritional tracks that can be adopted to improve both KOA and SO based on joint disease clinical severity.
Genomics and Antimicrobial Susceptibility of Clinical Pseudomonas aeruginosa Isolates from Hospitals in Brazil
Authors: Camargo Carlos Henrique , Yamada Amanda Yaeko , Souza Andreia Rodrigues de , Lima Marisa de Jesus de Castro , Cunha Marcos Paulo Vieira , Ferraro Pedro Smith Pereira , Sacchi Claudio Tavares , Santos Marlon Benedito Nascimento dos , Campos Karoline Rodrigues , Tiba-Casas Monique Ribeiro , Freire Maristela Pinheiro , Barretti Pasqual
Published: July 2023

Pseudomonas aeruginosa, an opportunistic pathogen causing infections in immunocompromised patients, usually shows pronounced antimicrobial resistance. In recent years, the frequency of carbapenemases in P. aeruginosa has decreased, which allows use of new beta-lactams/combinations in antimicrobial therapy. Therefore, the in vitro evaluation of these drugs in contemporary isolates is warranted. We evaluated the antimicrobial susceptibility and genomic aspects of 119 clinical P. aeruginosa isolates from 24 different hospitals in Brazil in 2021–2022. Identification was performed via MALDI-TOF-MS, and antimicrobial susceptibility was identified through broth microdilution, gradient tests, or disk diffusion. Whole-genome sequencing was carried out using NextSeq equipment. The most active drug was cefiderocol (100%), followed by ceftazidime–avibactam (94.1%), ceftolozane–tazobactam (92.4%), and imipenem–relebactam (81.5%). Imipenem susceptibility was detected in 59 isolates (49.6%), and the most active aminoglycoside was tobramycin, to which 99 (83.2%) isolates were susceptible. Seventy-one different sequence types (STs) were detected, including twelve new STs described herein. The acquired resistance genes blaCTX-M-2 and blaKPC-2 were identified in ten (8.4%) and two (1.7%) isolates, respectively. Several virulence genes (exoSTUY, toxA, aprA, lasA/B, plcH) were also identified. We found that new antimicrobials are effective against the diverse P. aeruginosa population that has been circulating in Brazilian hospitals in recent years.
Cutaneous Manifestations of Mycobacterium tuberculosis: A Literature Review
Authors: Nguyen Kevin H. , Alcantara Cheldon Ann , Glassman Ira , May Nicole , Mundra Akaash , Mukundan Abinanda , Urness Bianca , Yoon Sonyeol , Sakaki Roajhaan , Dayal Surbi , Chowdhury Tanzila , Harshavardhan Shakila , Ramanathan Vadakupattu , Venketaraman Vishwanath
Published: July 2023

Tuberculosis is an ancient disease that humanity struggled with for centuries and continues to struggle with. The bacteria Mycobacterium tuberculosis often infects the lungs through respiratory transmission and manifests itself through various symptoms, including cutaneous infections. Cutaneous tuberculosis (CTB) comprises about 1% to 1.5% of all extrapulmonary manifestations and is often accompanied by polymorphous lesions, including papules, nodules, plaques, ulcers, gummas, and verrucous lesions. CTB is most commonly observed in low-income, HIV, and immunosuppressed populations, similar to intrapulmonary manifestations. The main pathogen for CTB is M. tuberculosis but less commonly with M. bovis and BCG vaccine, and the modes of transmission are largely classified into exogenous and endogenous CTB. Current treatment options for CTB include oral therapy of antibiotic medications such as rifampicin, streptomycin, ethambutol, isoniazid, and pyrazinamide, which is occasionally combined with surgical intervention.
Tick-Borne Encephalitis Virus and Borrelia burgdorferi Seroprevalence in Balkan Tick-Infested Individuals: A Two-Centre Study
Authors: Jakimovski Dejan , Mateska Sofija , Dimitrova Emilija , Bosilkovski Mile , Mijatović Dragana , Simin Verica , Bogdan Ivana , Grujić Jasmina , Budakov-Obradović Zorana , Meletis Eleftherios , Kostoulas Polychronis , Cabezas-Cruz Alejandro , Banović Pavle
Published: July 2023

Lyme borreliosis (LB) and tick-borne encephalitis (TBE) are important tick-borne diseases in Europe. This study aimed to investigate the seroreactivity against Borrelia burgdorferi and TBE virus (TBEV) in tick-infested individuals in North Macedonia and Serbia. Serum samples were collected from tick-infested individuals and from healthy individuals in the same regions. Samples were tested for anti-Borrelia IgG reactivity and TBEV-neutralizing antibodies. Results showed higher seroreactivity against Borrelia antigens in patients and healthy donors from Novi Sad compared to those from the Skopje region. However, there was no statistically significant difference between tick-infested patients and healthy donors within each region. No TBEV-neutralizing antibodies were detected in participants from Novi Sad or in the control groups, except for one person from North Macedonia who had a moderate TBEV-neutralizing reaction. The study highlights the need for improved surveillance and diagnostic capabilities for LB and TBE in these regions. It also suggests the potential existence of TBEV foci in North Macedonia. The findings provide a complementary understanding of the LB and TBE epidemiology in the studied regions; however, further research is needed to investigate the presence and distribution of Borrelia spp. and TBEV in ticks to assess the significance of detected seroreactivity.
Luteolin Protects Pancreatic β Cells against Apoptosis through Regulation of Autophagy and ROS Clearance
Authors: Han Ming , Lu Yuting , Tao Yunhua , Zhang Xinwen , Dai Chengqiu , Zhang Bingqian , Xu Honghong , Li Jingya
Published: July 2023

Diabetes, which is mainly characterized by increased apoptosis and dysfunction of beta (β) cells, is a metabolic disease caused by impairment of pancreatic islet function. Previous studies have demonstrated that death-associated protein kinase-related apoptosis-inducing kinase-2 (Drak2) is involved in regulating β cell survival. Since natural products have multiple targets and often are multifunctional, making them promising compounds for the treatment of diabetes, we identified Drak2 inhibitors from a natural product library. Among the identified products, luteolin, a flavonoid, was found to be the most effective compound. In vitro, luteolin effectively alleviated palmitate (PA)-induced apoptosis of β cells and PA-induced impairment of primary islet function. In vivo, luteolin showed a tendency to lower blood glucose levels. It also alleviated STZ-induced apoptosis of β cells and metabolic disruption in mice. This function of luteolin partially relied on Drak2 inhibition. Furthermore, luteolin was also found to effectively relieve oxidative stress and promote autophagy in β cells, possibly improving β cell function and slowing the progression of diabetes. In conclusion, our findings show the promising effect of Drak2 inhibitors in relieving diabetes and offer a potential therapeutic target for the protection of β cells. We also reveal some of the underlying mechanisms of luteolin’s cytoprotective function.
Cardioprotective Agents for the Primary Prevention of Trastuzumab-Associated Cardiotoxicity: A Systematic Review and Meta-Analysis
Authors: Goulas Kyriakos , Farmakis Dimitrios , Constantinidou Anastasia , Kadoglou Nikolaos P. E.
Published: July 2023

There are significant considerations about the prevention of cardiotoxicity caused by trastuzumab therapy in patients with breast cancer, leading to discontinuation. Recently, randomized controlled trials (RCTs) have evaluated the effects of early commitment of beta-blockers (BBs), angiotensin receptor blockers (ARBs) and angiotensin converting enzyme inhibitors (ACEIs) during trastuzumab chemotherapy in order to prevent the related cardiotoxicity. The present systematic review and meta-analysis of six RCTs included patients who have predominantly non-metastatic, HER2-positive, breast cancer and received trastuzumab as primary or adjuvant therapy. Those patients did not have any obvious cardiac dysfunction or any previous therapy with cardioprotective agent. We evaluated the efficacy of the aforementioned medications for primary prevention of cardiotoxicity, using random effects models. Any preventive treatment did not reduce cardiotoxicity occurrence compared to controls (Odds ratios (OR) = 0.92, 95% CI 0.54–1.56, p = 0.75). Results were similar for ACEIs/ARBs and beta-blockers. Treatment with ACEIs/ARBs led to a slight, but significant, increase in LVEF in patients compared to the placebo group. Only two studies reported less likelihood of discontinuation of trastuzumab treatment. More adequately powered RCTs are needed to determine the efficacy of routine prophylactic therapy.
Recent Progress in Extracellular Vesicle-Based Carriers for Targeted Drug Delivery in Cancer Therapy
Authors: Tang Yaqin , Liu Xingyou , Sun Meng , Xiong Su , Xiao Nianting , Li Jianchao , He Xiao , Xie Jing
Published: July 2023

Extracellular vesicles (EVs) are small, membrane-based vesicles released by cells that play a critical role in various physiological and pathological processes. They act as vehicles for transporting a variety of endogenous cargo molecules, enabling intercellular communication. Due to their natural properties, EVs have emerged as a promising “cell-free therapy” strategy for treating various diseases, including cancer. They serve as excellent carriers for different therapeutics, including nucleic acids, proteins, small molecules, and other nanomaterials. Modifying or engineering EVs can improve the efficacy, targeting, specificity, and biocompatibility of EV-based therapeutics for cancer therapy. In this review, we comprehensively outline the biogenesis, isolation, and methodologies of EVs, as well as their biological functions. We then focus on specific applications of EVs as drug carriers in cancer therapy by citing prominent recent studies. Additionally, we discuss the opportunities and challenges for using EVs as pharmaceutical drug delivery vehicles. Ultimately, we aim to provide theoretical and technical support for the development of EV-based carriers for cancer treatment.
Comparison of Bovine- and Porcine-Derived Decellularized Biomaterials: Promising Platforms for Tissue Engineering Applications
Authors: El-Husseiny Hussein M. , Mady Eman A. , Kaneda Masahiro , Shimada Kazumi , Nakazawa Yasumoto , Usui Tatsuya , Elbadawy Mohamed , Ishihara Yusuke , Hirose Moeko , Kamei Yohei , Doghish Ahmed S. , El-Mahdy Hesham A. , El-Dakroury Walaa A. , Tanaka Ryou
Published: July 2023

Animal-derived xenogeneic biomaterials utilized in different surgeries are promising for various applications in tissue engineering. However, tissue decellularization is necessary to attain a bioactive extracellular matrix (ECM) that can be safely transplanted. The main objective of the present study is to assess the structural integrity, biocompatibility, and potential use of various acellular biomaterials for tissue engineering applications. Hence, a bovine pericardium (BP), porcine pericardium (PP), and porcine tunica vaginalis (PTV) were decellularized using a Trypsin, Triton X (TX), and sodium dodecyl sulfate (SDS) (Trypsin + TX + SDS) protocol. The results reveal effective elimination of the cellular antigens with preservation of the ECM integrity confirmed via staining and electron microscopy. The elasticity of the decellularized PP (DPP) was markedly (p < 0.0001) increased. The tensile strength of DBP, and DPP was not affected after decellularization. All decellularized tissues were biocompatible with persistent growth of the adipose stem cells over 30 days. The staining confirmed cell adherence either to the peripheries of the materials or within their matrices. Moreover, the in vivo investigation confirmed the biocompatibility and degradability of the decellularized scaffolds. Conclusively, Trypsin + TX + SDS is a successful new protocol for tissue decellularization. Moreover, decellularized pericardia and tunica vaginalis are promising scaffolds for the engineering of different tissues with higher potential for the use of DPP in cardiovascular applications and DBP and DPTV in the reconstruction of higher-stress-bearing abdominal walls.
Environmentally Friendly Strategies for Formulating Vegetable Oil-Based Nanoparticles for Anticancer Medicine
Authors: Freire Nathália , Barbosa Raquel de Melo , García-Villén Fátima , Viseras César , Perioli Luana , Fialho Rosana , Albuquerque Elaine
Published: July 2023

The development of green synthesized polymeric nanoparticles with anticancer studies has been an emerging field in academia and the pharmaceutical and chemical industries. Vegetable oils are potential substitutes for petroleum derivatives, as they present a clean and environmentally friendly alternative and are available in abundance at relatively low prices. Biomass-derived chemicals can be converted into monomers with a unique structure, generating materials with new properties for the synthesis of sustainable monomers and polymers. The production of bio-based polymeric nanoparticles is a promising application of green chemistry for biomedical uses. There is an increasing demand for biocompatible and biodegradable materials for specific applications in the biomedical area, such as cancer therapy. This is encouraging scientists to work on research toward designing polymers with enhanced properties and clean processes, containing oncology active pharmaceutical ingredients (APIs). The nanoencapsulation of these APIs in bio-based polymeric nanoparticles can control the release of the substances, increase bioavailability, reduce problems of volatility and degradation, reduce side effects, and increase treatment efficiency. This review discusses the use of green chemistry for bio-based nanoparticle production and its application in anticancer medicine. The use of castor oil for the production of renewable monomers and polymers is proposed as an ideal candidate for such applications, as well as more suitable methods for the production of bio-based nanoparticles and some oncology APIs available for anticancer application.
Application of Nanomedicine in Immunotherapy: Recent Advances and Prospects
Authors: Longo João Paulo Figueiró , Muehlmann Luis Alexandre
Published: July 2023

Nanomedicine is a special medical field focused on the application of nanotechnology to provide innovations for healthcare in different areas, including the treatment of a wide variety of diseases, including cancer [...]
Suppressing the Zero-Frequency Components in Single Quantitative Phase Imaging by Filtering the Low-Frequency Intrinsic Mode Function Components
Authors: Long Jiale , Meng Chuisong , Ding Yi , Xi Jiangtao
Published: July 2023

In off-axis quantitative phase imaging, existing methods of suppressing the zero-frequency component (ZFC) always cause the loss of high-frequency phase information, thus degrading the accuracy of phase reconstruction. To overcome these problems, this paper proposes to preserve the high-frequency information by filtering the intrinsic mode function. In this method, empirical mode decomposition is employed to decompose the interferometric image into a series of intrinsic mode function (IMF) components from high to low frequencies. The decomposed low-frequency IMF components are processed by Gaussian high-pass filters for ZFC suppression, and the high-frequency IMF components and the filtered low-frequency IMF components are combined to obtain the reconstructed hologram. Hilbert transform is then performed on the reconstructed hologram to filter out the conjugate image, leaving only the original image. In order to verify the performance of our proposed method, the phase maps processed by our proposed method are compared with those processed by the Fourier filtering method, wavelet transform-based method and Laplace operator method. The experimental results show that the proposed method not only suppresses ZFC but also achieves higher accuracy in phase reconstruction.
Genome-Wide Identification and Functional Characterization of FAR1-RELATED SEQUENCE (FRS) Family Members in Potato (Solanum tuberosum)
Authors: Chen Qingshuai , Song Yang , Liu Kui , Su Chen , Yu Ru , Li Ying , Yang Yi , Zhou Bailing , Wang Jihua , Hu Guodong
Published: July 2023

FAR1-RELATED SEQUENCE (FRS) transcription factors are generated by transposases and play vital roles in plant growth and development, light signaling transduction, phytohormone response, and stress resistance. FRSs have been described in various plant species. However, FRS family members and their functions remain poorly understood in vegetative crops such as potato (Solanum tuberosum, St). In the present study, 20 putative StFRS proteins were identified in potato via genome-wide analysis. They were non-randomly localized to eight chromosomes and phylogenetic analysis classified them into six subgroups along with FRS proteins from Arabidopsis and tomato. Conserved protein motif, protein domain, and gene structure analyses supported the evolutionary relationships among the FRS proteins. Analysis of the cis-acting elements in the promoters and the expression profiles of StFRSs in various plant tissues and under different stress treatments revealed the spatiotemporal expression patterns and the potential roles of StFRSs in phytohormonal and stress responses. StFRSs were differentially expressed in the cultivar “Xisen 6”, which is exposed to a variety of stresses. Hence, these genes may be critical in regulating abiotic stress. Elucidating the StFRS functions will lay theoretical and empirical foundations for the molecular breeding of potato varieties with high light use efficiency and stress resistance.
Characterization of Targeted Phenolic Compounds in Globe Artichoke Heads and Waste from Vegetatively and “Seed”-Propagated Genotypes
Authors: Bonasia Anna , Conversa Giulia , Lazzizera Corrado , Elia Antonio
Published: July 2023

In the globe artichoke, both the edible portion and the waste biomass are recognized as valuable sources of bioactive compounds. For this study, heads with 30 cm–long floral stems including two to three leaves were harvested from five genotypes, which included two traditional vegetative-propagated varietal types (“Brindisino” and “Violetto di Foggia”) and three “seed”-propagated hybrids (“Tempo”, “Opal”, and “Madrigal”). The study aimed to determine the total and individual polyphenolic concentrations (measured spectrophotometrically and using HPLC) and antioxidant activity (AA) in different artichoke parts, namely the “hearts” (H), head waste (HW), stem waste (SW), and leaf waste (LW). “Brindisino” SW exhibited the highest accumulation of luteolin (26,317 mg kg−1 F.W.), while “Tempo” H displayed the highest cynarin content (190 mg kg−1 F.W.). “Tempo” HW and H showed the highest levels of apigenin (640 mg kg−1 F.W.), and the greatest source of chlorogenic acid was found in the HW of “Opal” and the H of “Brindisino” (4300 mg kg−1 F.W.). The hybrids generally exhibited lower total polyphenolic concentrations than the traditional genotypes, particularly evident in the LW. The SW demonstrated the highest concentration of total polyphenols (18,000 mg kg−1 F.W.), followed by the edible H and non-edible HW (12,000 mg kg−1 F.W.), while the LW exhibited the lowest concentration (2000 mg kg−1 F.W.). Interestingly, the AA did not precisely align with the total polyphenolic concentration, showing slight variations between the examined parts and genotypes.
Identification and Expression Analysis of DFR Gene Family in Brassica napus L.
Authors: Qian Xingzhi , Zheng Wenyin , Hu Jian , Ma Jinxu , Sun Mengyuan , Li Yong , Liu Nian , Chen Tianhua , Wang Meiqi , Wang Ling , Hou Xinzhe , Cai Qingao , Ye Zhaoshun , Zhang Fugui , Zhu Zonghe
Published: July 2023

Dihydroflavonol 4-reductase (DFR) is a key enzyme in the flavonoid biosynthetic pathway and is essential for the formation of plants’ color. In this study, 26 BnDFR genes were identified using 6 Arabidopsis DFR genes as reference. The physicochemical properties, subcellular localization, and conserved structure of BnDFR proteins were analyzed; the evolutionary relationship, collinearity analysis, and expression characteristics of BnDFR genes were studied; and the correlation between the expression level of BnDFR genes and anthocyanin content in rape petals were analyzed. The results showed that the 26 BnDFRs were located in chloroplasts, cytoplasm, nuclei, and mitochondria, distributed on 17 chromosomes, and divided into 4 groups; members of the same group have a similar function, which may be related to the environmental response elements and plant hormone response elements. Intraspecific collinearity analysis showed 51 pairs of collinear genes, and interspecific collinearity analysis showed 30 pairs of collinear genes. Analysis of the expression levels of BnDFRs and anthocyanin content in different color rape petals showed that BnDFR6 and BnDFR26 might play an important role in the synthesis of anthocyanins in rape petals. This provides theoretical guidance for further analysis of the anthocyanin anabolism mechanism involved in the DFR gene in Brassica napus.
Effect of Climate and Competition on Radial Growth of Pinus sylvestris var. mongolica Forest in Hulunbuir Sandy Land of Inner Mongolia, China
Authors: Wen Shuo , Shi Zhongjie , Zhang Xiao , Pan Leilei , Kwon Semyung , Li Yuheng , Yang Xiaohui , Li Hanzhi
Published: July 2023

(1) Background: The forest of Pinus sylvestris var. mongolica is an important semi-arid ecosystem in Hulunbuir sandy land that plays a key role in the carbon cycle and wind erosion control. It is crucial to explore the main factors affecting the radial growth of trees of P. sylvestris var. mongolica. (2) Methods: The study established the tree-ring chronology of P. sylvestris var. mongolica and analyzed the relationships among the radial growth, competition index, and climate variables using correlation analysis and a linear mixed effect model to explore the influence of competition and climate on radial growth of P. sylvestris var. mongolica. (3) Results: The results indicated that tree growth is mainly affected by the maximum average temperature (Tmax) and precipitation in June and July of the current year and that tree growth significantly decreased with increasing competition pressure. Analysis of the linear mixed effect model showed that tree age, competition intensity, self-calibrating Palmer drought severity index (scPDSI) from May to July, and vapor pressure deficit (VPD) have a significant impact on radial growth. (4) Conclusions: The competition plays a dominant role in radial growth of P. sylvestris var. mongolica compared to climate factors. This study helps to understand the growth mechanism of P. sylvestris var. mongolica forests under climate change and provides a scientific basis for effective management of semi-arid forests.
Protein–Protein Interactions and Quantitative Phosphoproteomic Analysis Reveal Potential Mitochondrial Substrates of Protein Phosphatase 2A-B’ζ Holoenzyme
Authors: Elshobaky Ahmed , Lillo Cathrine , Hodén Kristian Persson , Kataya Amr R. A.
Published: July 2023

Protein phosphatase 2A (PP2A) is a heterotrimeric conserved serine/threonine phosphatase complex that includes catalytic, scaffolding, and regulatory subunits. The 3 A subunits, 17 B subunits, and 5 C subunits that are encoded by the Arabidopsis genome allow 255 possible PP2A holoenzyme combinations. The regulatory subunits are crucial for substrate specificity and PP2A complex localization and are classified into the B, B’, and B” non-related families in land plants. In Arabidopsis, the close homologs B’η, B’θ, B’γ, and B’ζ are further classified into a subfamily of B’ called B’η. Previous studies have suggested that mitochondrial targeted PP2A subunits (B’ζ) play a role in energy metabolism and plant innate immunity. Potentially, the PP2A-B’ζ holoenzyme is involved in the regulation of the mitochondrial succinate/fumarate translocator, and it may affect the enzymes involved in energy metabolism. To investigate this hypothesis, the interactions between PP2A-B’ζ and the enzymes involved in the mitochondrial energy flow were investigated using bimolecular fluorescence complementation in tobacco and onion cells. Interactions were confirmed between the B’ζ subunit and the Krebs cycle proteins succinate/fumarate translocator (mSFC1), malate dehydrogenase (mMDH2), and aconitase (ACO3). Additional putative interacting candidates were deduced by comparing the enriched phosphoproteomes of wild type and B’ζ mutants: the mitochondrial regulator Arabidopsis pentatricopeptide repeat 6 (PPR6) and the two metabolic enzymes phosphoenolpyruvate carboxylase (PPC3) and phosphoenolpyruvate carboxykinase (PCK1). Overall, this study identifies potential PP2A substrates and highlights the role of PP2A in regulating energy metabolism in mitochondria.
Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas
Authors: Farooq Nisma , Khan Muhammad Omar , Ahmed Muhammad Zaheer , Fatima Samia , Nawaz Muhammad Asif , Abideen Zainul , Nielsen Brent L. , Ahmad Niaz
Published: July 2023

Brassica species show varying levels of resistance to salt stress. To understand the genetics underlying these differential stress tolerance patterns in Brassicas, we exposed two widely cultivated amphidiploid Brassica species having different genomes, Brassica juncea (AABB, n = 18) and Brassica napus (AACC, n = 19), to elevated levels of NaCl concentration (300 mM, half the salinity of seawater). B. juncea produced more biomass, an increased chlorophyll content, and fewer accumulated sodium (Na+) and chloride (Cl−) ions in its photosynthesizing tissues. Chlorophyll fluorescence assays revealed that the reaction centers of PSII of B. juncea were more photoprotected and hence more active than those of B. napus under NaCl stress, which, in turn, resulted in a better PSII quantum efficiency, better utilization of photochemical energy with significantly reduced energy loss, and higher electron transport rates, even under stressful conditions. The expression of key genes responsible for salt tolerance (NHX1 and AVP1, which are nuclear-encoded) and photosynthesis (psbA, psaA, petB, and rbcL, which are chloroplast-encoded) were monitored for their genetic differences underlying stress tolerance. Under NaCl stress, the expression of NHX1, D1, and Rubisco increased several folds in B. juncea plants compared to B. napus, highlighting differences in genetics between these two Brassicas. The higher photosynthetic potential under stress suggests that B. juncea is a promising candidate for genetic modifications and its cultivation on marginal lands.
Integrative Metabolic and Transcriptomic Profiling in Camellia oleifera and Camellia meiocarpa Uncover Potential Mechanisms That Govern Triacylglycerol Degradation during Seed Desiccation
Authors: Chen Mingjie , Zhang Yi , Du Zhenghua , Kong Xiangrui , Zhu Xiaofang
Published: July 2023

Camellia seed oil is a top-end quality of cooking oil in China. The oil quality and quantity are formed during seed maturation and desiccation. So far, it remains largely unresolved whether lipid degradation occurs and contributes to Camellia oil traits. In this study, three different Camellia germplasms, C. oleifera cv. Min 43 (M43), C. meiocarpa var. Qingguo (QG), and C. meiocarpa cv Hongguo (HG) were selected, their seed oil contents and compositions were quantified across different stages of seed desiccation. We found that at the late stage of desiccation, M43 and QG lost a significant portion of seed oil, while such an event was not observed in HG. To explore the molecular bases for the oil loss In M43, the transcriptomic profiling of M43 and HG was performed at the early and the late seed desiccation, respectively, and differentially expressed genes (DEGs) from the lipid metabolic pathway were identified and analyzed. Our data demonstrated that different Camellia species have diverse mechanisms to regulate seed oil accumulation and degradation, and that triacylglycerol-to-terpenoid conversion could account for the oil loss in M43 during late seed desiccation.
Co-Inoculation with Arbuscular Mycorrhizal Fungi and Dark Septate Endophytes under Drought Stress: Synergistic or Competitive Effects on Maize Growth, Photosynthesis, Root Hydraulic Properties and Aquaporins?
Authors: Gong Minggui , Bai Na , Wang Pengfei , Su Jiajie , Chang Qingshan , Zhang Qiaoming
Published: July 2023

Arbuscular mycorrhizal fungi (AMF) and dark septate fungi (DSE) were simultaneously colonized in the root cells of maize. Single AMF and DSE symbiosis have been proven to improve the drought tolerance of maize. However, the effects of both fungi coexisting in maize roots under drought stress are not yet known. In this study, pot experiments of maize seedlings were conducted through four inoculation treatments (single AMF inoculation of Rhizophagus irregularis, single DSE inoculation of Exophiala pisciphila, co-inoculation of AMF + DSE and non-mycorrhizal inoculation) under well-watered (WW) and drought-stressed (DS) conditions. AMF and DSE colonization status, maize physiology and aquaporin gene expression in maize roots were investigated. The objective of this paper was to evaluate whether AMF and DSE had competitive, independent or synergistic effects on regulating the drought tolerance of maize. When maize seedlings of three inoculation treatments were subjected to drought stress, single AMF inoculation had the highest shoot and root dry weight, plant height, root length, osmotic root hydraulic conductivity and hydrostatic root hydraulic conductivity in maize seedlings. However, co-inoculation of AMF + DSE induced the highest stomatal conductance in maize leaves and the lowest H2O2 and O2•− concentration, membrane electrolyte leakage, intercellular CO2 concentration and gene expression level of ZmPIP1;1, ZmPIP1;2, ZmPIP2;1, ZmPIP2;5 and ZmPIP2;6. In addition, co-inoculation of AMF + DSE also obviously down-regulated the GintAQPF1 and GintAQPF2 expression in R. irregularis compared with single AMF inoculation treatment. Under DS stress, there were competitive relationships between AMF and DSE with regard to regulating mycorrhizal colonization, maize growth, root hydraulic conductivity and the gene expression of aquaporins in R. irregularis, but there were synergistic relationships with regard to regulating membrane electrolyte leakage, oxidative damage, photosynthesis and the aquaporin gene expression of maize seedlings. The obtained results improve our knowledge about how the mechanisms of AMF and DSE coexist, promoting the drought tolerance of host plants.
Control Efficacy of UAV-Based Ultra-Low-Volume Application of Pesticide in Chestnut Orchards
Authors: Arakawa Takumi , Kamio Shinji
Published: July 2023

Pesticide spraying using unmanned aerial vehicles (UAVs) has been utilized in many crops, including fruit tree crops, because of its merits in terms of labor-saving and the low risk to the operator. However, its relevance to chestnut, one of the commercially significant fruit trees grown throughout Europe and Asia, has not been studied. In this work, we assessed the effectiveness of UAV-based ultra-low-volume pesticide application in chestnuts. We demonstrated the efficiency of three insecticides applied by a UAV on young chestnut trees. Interestingly, using a reduced amount of one of the pesticides, UAV-based spraying had greater control efficacy than conventional methods. The efficacy of ultra-low-volume pesticide application to adult trees was equivalent to using an air-blast sprayer. The spray coverage was compared in terms of spray volume (20 L vs. 40 L ha−1), flight method (straight flight vs. rotating flight for each tree), the size of the UAVs (8 L vs. 30 L in payload capacity), flow rate (3.8 L vs. 6.0 L min−1), and tree age in order to characterize the droplet deposition of UAV-based spraying. Overall, we showed that spraying pesticides using a UAV could effectively protect chestnut trees. It was debated how tree training, or tree height, affected pest control.
Single-Bud Expression Analysis of Bud Dormancy Factors in Peach
Authors: Puertes Ana , Polat Helin , Ramón-Núñez Luis Andrés , González Matilde , Ancillo Gema , Zuriaga Elena , Ríos Gabino
Published: July 2023

Transcriptomic and gene expression analysis have greatly facilitated the identification and characterization of transcriptional regulatory factors and effectors involved in dormancy progression and other physiological processes orchestrated during bud development in peach and other temperate fruit species. Gene expression measurements are most usually based on average values from several or many individual buds. We have performed single-bud gene analysis in flower buds of peach across dormancy release using amplicons from the master regulatory DORMANCY-ASSOCIATED MADS-BOX (DAM) factors, several jasmonic acid biosynthetic genes, other genes related to flowering development, cell growth resumption, and abiotic stress tolerance. This analysis provides a close view on gene-specific, single-bud variability throughout the developmental shift from dormant to dormancy-released stages, contributing to the characterization of putative co-expression modules and other regulatory aspects in this particular tissue.
Optimization of the Electrospray Process to Produce Lignin Nanoparticles for PLA-Based Food Packaging
Authors: Daassi Rodrigue , Durand Kalvin , Rodrigue Denis , Stevanovic Tatjana
Published: July 2023

The development of new processing methods is required in order to meet the continuous demand for thinner films with excellent barrier properties for food packaging and other applications. In this study, rice husk organosolv lignin nanoparticles were prepared using the electrospray method, which were applied to produce polylactic acid (PLA)-based films for food packaging. The effect of the following electrospray parameters has been investigated: lignin concentration (LC) ranging from 5–50 mg/mL, flow rate (FR) from 0.5–1 mL/min, applied voltage from 10–30 kV, and tip-to-collector distance (TCD) from 10–25 cm, on the morphology, size, polydispersity index (PDI), and Zeta potential (ZP) of lignin nanoparticles (LNPs). The response surface methodology with a Box-Behnken design was applied to optimize these parameters, while dynamic light scattering (DLS) and scanning electron microscopy (SEM) analyses were used to characterize the controlled LNPs. The results showed that the LNPs shape and sizes represent a balance between the solvent evaporation, LC, applied voltage, TCD and FR. The application of optimal electrospray conditions resulted in the production of LNPs with a spherical shape and a minimal size of 260 ± 10 nm, a PDI of 0.257 ± 0.02, and a ZP of −35.2 ± 4.1 mV. The optimal conditions were achieved at LC = 49.1 mg/mL and FR = 0.5 mL/h under an applied voltage of 25.4 kV and TCD = 22 cm. Then, the optimized LNPs were used to improve the properties of PLA-based films. Three types of PLA-lignin blend films were casted, namely lignin/PLA, LNPs/PLA and PLA-grafted LNPs. PLA-grafted LNPs exhibited a more uniform dispersion in PLA for lignin contents of up to 10% than other composite samples. Increasing the lignin content from 5% to 10% in PLA-grafted LNPs resulted in a significant increase in elongation at break (up to four times higher than neat PLA). The presence of PLA-grafted lignin led to a substantial reduction in optical transmittance in the UV range, dropping from 58.7 ± 3.0% to 1.10 ± 0.01%, while maintaining excellent transparency to visible light compared to blends containing lignin or LNPs. Although the antioxidant capacity of unmodified lignin is well-known, a substantial increase in antioxidant capacity was observed in LNPs and PLA-grafted LNP films, with values exceeding 10 times and 12 times that of neat PLA, respectively. These results confirm the significant potential of using studied films in food packaging applications.
Synthesis and Characterization of an Environmentally Friendly Phenol–Formaldehyde Resin Modified with Waste Plant Protein
Authors: Li Hanyin , Wang Sen , Zhang Xiang , Wu Hao , Wang Yujie , Zhou Na , Zhao Zijie , Wang Chao , Zhang Xiaofan , Wang Xian , Li Cheng
Published: July 2023

To develop a lower-cost, excellent-performance, and environmentally friendly phenol–formaldehyde (PF) resin, soybean meal was used to modify PF resin, and soybean meal–phenol–formaldehyde (SMPF) resins were prepared. This reveals the effect of soybean meal on the structural, bonding, and curing properties of PF resin, which are very important for its applications in the wood industry. The resins’ physicochemical properties and curing performance were investigated, showing that SMPF resins have higher curing temperatures than PF resin. The Fourier transform infrared spectroscopy results indicated that a cross-linking reaction occurred between the amino groups of soybean protein and the hydroxymethyl phenol. Moreover, with the addition of soybean meal, the viscosity of SMPF increased while the gel time decreased. It is worth mentioning that SMPF-2 resin has favorable viscosity, short gel time, low curing temperature (135.78 °C), and high water resistance and bonding strength (1.01 MPa). Finally, all the plywoods bonded with SMPF resins have good water resistance and bonding strength, which could meet the standard (GB/T 17657-2013, type I) for plywood. The optimized SMPF resins showed the potential for application to partially replace PF resin in the wood industry.