bluuebunny/crossref_metadata_2025 huggingface dataset:
Glycation and α-glucosidase inhibitors from boesenbergia rotunda and lansium parasiticum
Authors:   , Potipiranun Thammatee
Published: September 2023

Glycation is spontaneous non-enzymatic reaction between protein and glycation precursors; methylglyoxal (MG) that initially yields advanced glycation end-products (AGEs), which ultimately trigger to several severe complications. Therefore, the inhibition of AGEs formation is the imperative approach for alleviating diabetic complications. Moreover, one of typical diabetic therapy is α-glucosidase inhibition to reduce glucose level in bloodstream. Therefore, this research aims to study bioactivity and mechanism of natural products to inhibit glycation and α-glucosidase. From preliminary study of some phenolic compounds, flavanones from fingerroot (Boesenbergia rotunda) could inhibit glycation. The dichloromethane extract of fingerroot afforded three flavanones (pinocembrin, pinostrobin, and alpinetin), two chalcones (cardamomin and boesenbergin B), two dihydrochalcones (panduratin A and isopanduratin A), and one kavalactone (demethoxyyangonin). Most of isolated compounds showed higher AGEs formation inhibition than aminoguanidine (AG). Subsequent evaluation in MG-trapping assay indicated that their trapping potency was relatively comparable to AG. Their structure-activity relationships (SAR) of MG-trapping activity were investigated using the comparison of the structures of flavonoids. In addition, this is the first time that pinocembrin was tested ⍺-glucosidase inhibitory activity. In this study, we found a promising α-glucosidase inhibition (IC50 2.5-4.3 mg/mL) in the extract of Lansium parasiticum (syn L. domesticum; Meliaceae) fruit peels, which is a popular fruit in South-east Asia. It has been recognized as an important source of diverse triterpenoids, particularly a rare and unique skeleton of onoceranoids. A novel onoceranoid triterpene, named lamesticumin G along with four known compounds (lansionic acid, 3β-hydroxyonocera-8(26),14-dien-21-one, methyl lansiolate and lansiolic acid) were isolated from the ethyl acetate extract of the fruit peels of Lansium parasiticum. The structure of lamesticumin G was fully characterized using spectroscopic data. Lamesticumin G inhibited α-glucosidase (maltase) with IC50 value of 2.27 mM, while other compounds showed no inhibition.
Removal of mixed VOCs of benzene, Toluene and xylene by a multistage corona discharge system
Authors:   , Ditthawat Nitikorn
Published: September 2023

The oxidation of mixed volatile organic compounds (VOCs) of benzene, tol-uene and xylene in non-thermal plasma corona discharge was investigated. An initial total VOCs concentration of 100 ppm and feed flow rate of 50.0 ml/min were firstly fixed to investigate the effects of an applied voltage and input frequency on VOCs conversion, power consumption and CO₂ and CO selectivities. For a single stage plasma system, under the optimum applied voltage of 8 kV and input frequency of 500 Hz, the system gave the high CO₂ selectivity with the low CO selectivity and the lowest power consumption. At the optimum operationable conditions, the effect of reactant feed flow rate and stage number were examined. The increase in reactant feed flow rate from 25 to 125 ml/min corresponding to the reduction of residence time from 0.92 to 0.19 s resulted in decreases in both of VOCs conversion and CO₂ selectivity from 97.7 to 63.9% and 84.0 to 22.6%, respectively while the CO selectivity was increased from 8.2 to 62.4%. An increase in stage number from one to two stages caused an increase in the total VOCs conversion approximately 100% especially for the feed flow rate of 100 ml/min.
Kinetics study for fischer-tropsch synthesis over cobalt catalyst by kinetic monte-carlo simulation
Authors:   , Pugingna Nuttawut
Published: September 2023

Kinetic Monte-Carlo simulation was utilized to study the kinetic behaviors of C₁ and C₂ formation in the Fischer-Tropsch synthesis over the cobalt surface by varying reaction conditions. Two reaction models: the carbide mechanism and the CO insertion mechanism were applied to this simulation. The production rates and fractional coverages of each adsorbed species could be calculated under the steady state. Moreover, the combination between carbide and CO insertion mechanism was also studied to suggest the possibility of each reaction mechanism, and compared the results with the reported experimental results under the same conditions. It was ob-served that the C–C bond formation was mainly performed through the insertion of adsorbed CO into a metal–methyl bond. Based on the CO insertion mechanism, the results obviously presented that the process conditions essentially influenced on the reactivity and selectivity of the reaction. The system exhibited non-reactive regions at the temperature below 463 K and the H₂/CO feed molar ratio lower than 1.1. In the steady reactive states, the surface is predominantly covered with adsorbed H and CO, and played an important role in the hydrocarbon chain growth.
Effecst of water on the methane hydrate formation and dissociation with hollow silica and activated carbon
Authors:   , Rungrussamee Sarocha
Published: September 2023

The porous materials, hollow silica (HSC) and activated carbon (AC), were selected to investigate their effects with water on methane hydrate formation and dissociation. The methane hydrate formation with the porous materials was conducted at 8 MPa and 277 K. The dissociation of hydrates was achieved by thermal stimulation with 294 K temperature driving force. The result showed that the hydrate formation of the system with 1:14 HSC to water ratio, the system with excess water, provided the highest methane consumed and water conversion to hydrates. That is because the HSC has low density allowing it to float on water and small particle size leading to the highest volume of water in the system. However, the excess water increased the mass transfer resistance obstructing the gas to dissolve into the water. The system with 1:0.8 AC to water ratio, water deficit, had the lowest methane consumed and water conversion to hydrates. That is because its hydrophilic nature and capillary effect caused by AC small pores. This resulted in the insufficient water to convert to hydrates and led to the low methane consumed. However, the AC has a large surface area and can adsorb methane. And that may aid the gas solubility and fast induction time of the system with the AC. The final methane recovery was about the same in the systems with the HSC and AC at the same temperature driving force (294 K). Therefore, the amount of water and type of porous materials could play important roles on the methane hydrate formation.
Taxonomic revision and pollination biology of orchid genera Crepidium Blume and Dienia Lindl. (MALAXIDINAE, ORCHIDACEAE) in Thailand
Authors:   , Nuammee Anchalee
Published: September 2023

The terrestrial orchid Crepidium Blume and Dienia Lindl. in Thailand were enumerated by Gunnar Seidenfaden under Malaxis with key and drawings. Because of no species descriptions in publication, it is difficult to delimit species boundary. Moreover, recent discovery of new species results in unreliable assessment of species, therefore, the taxonomic revision of Crepidium and Dienia in Thailand should be carried out. The results indicated that the column and transversal callus on labellum are taxonomically important characters to delimit these two genera. In addition, morphological characters, i.e. stem and leaf morphology, labellum auricles, margins and number of teeth on distal margin of labellum, and high ridges on labellum cavity, are useful characters to classify and identify to species. It is suggested that only 1 species of Dienia and 25 species of Crepidium are distributed in Thailand. Four species are new records for Thailand, namely, C. bahanense (Hand.-Mazz.) S. C. Chen & J. J. Wood, C. josephianum (Rchb. f.) Marg., C. maximowiczianum (King & Pantl.) Szlach. and C. micranthum (Hook. f.) Szlach. and a new species, C. falcifolium Nuammee, Seelanan & H. A. Pedersen. Furthermore, the two new combinations are made and substantiated, i.e. C. chamaeorchis (Schltr.) Nuammee, Seelanan, Suddee & H. A. Pedersen and C. szemaoense (Tang & F. T. Wang) Nuammee, Seelanan, Suddee & H. A. Pedersen.

The investigation on pollination biology of C. acuminatum (D. Don) Szlach and D. ophrydis (J. Koenig) Ormerod & Seidenf. indicated that C. acuminatum is likely cross-pollinated orchid that relies on pollinators for successful pollination. In contrary, D. ophrydis is autonomous self-pollination assisted by rain whose pollination mechanism was assisted by viscous drops and water adhesion. Both C. acuminatum and D. ophrydis produced and secreted nectar to possibly attract pollinators. The much lower percentages of natural fruit set of C. acuminatum may be due to self-incompatibility, pollinator limitation, coupling with other environmental factors. Whilst, D. ophrydis may employ nectar as attractant to pollinators in order to increasing chance for outcrossing but autonomous self-pollination assisted by rain thus seem to be an important strategy that guarantees fruit set when pollinator’s visits are scarce.
Conversion of n-Pentane to aromatics: effect of surface modification by chemical liquid deposition and promoter over nano scale Ga/HZSM-5
Authors:   , Sintapanin Sukkatad
Published: September 2023

This work studied the effects of chemical liquid deposition, i.e., TEOS concentration and multicycle silylation on Ga/HZSM-5 on their catalytic performance in aromatization of n-pentane. The parent nano scale HZSM-5 zeolite catalysts were incorporated with Ga by impregnation method followed by silylation via chemical liquid deposition (CLD) using tetraethyl orthosilicate (TEOS) by varying concentrations and number of cycles. Moreover, the small amount of Pt, Zn, La and P was introduced by co-impregnation method to study the effect of promoter. The physical and chemical properties of catalysts were characterized by N2 physisorption, XRD, NH3-TPD, IPA-TPD, TPO, TPR, SEM and 27Al MAS NMR techniques. Catalytic activity was tested in a continuous flow fixed-bed reactor at 500 °C, atmospheric pressure, and WHSV of 5 h-1. The results indicated that the addition of Ga greatly improved the aromatics selectivity due to the presence of GaO+ species which acted as active site for dehydrogenation reaction. The TEOS concentration slightly increased the p-xylene selectivity while multicycle silylation significantly improved the p-xylene in mixed xylenes due to the formation of uniformity inert silica layer. The silylated catalyst exhibited the lower of n-pentane conversion resulting from its lower external acid sites. The co-impregnation with P promoted the aromatics selectivity because there was formation of SAPO interfaces, pentahedrally aluminium framework and medium acid sites.
Development of group-contribution models for use in surfactant selection – case studies of single and mixed surfactant applications
Authors:   , Chanachichalermwong Woramet
Published: September 2023

Microemulsion is the surfactant/oil/water system that is applied to many applications such as enhanced oil recovery, cleaning agents, environmental remediation and drug delivery system. To select the suitable surfactant to form an efficient microemulsion system for each specific condition and application, a systematic selection method is needed to reduce time and valuable resources that may be required in the design of an interest product. This work developed a systematic method to select suitable surfactants for a specific application based on several properties including but not limited to types of emulsion, emulsion stability, solubility and toxicology. Since not all models are available in the literature, this work developed properties models based on the molecular structure of the surfactants by using the concept of Group-Contribution (GC) based on Marrero and Gani method. One important key of surfactant properties for Hydrophilic-Lipophilic property through an Hydrophilic-Lipophilic Deviation (HLD) equation is the characterictic curvature (Cc) that indicates the hydrophobicity or hydrophilicity of a surfactant based on their molecular structure. This work developed the GC-model for prediction of characteristic curvature of anionic and nonionic surfactants to use in the HLD equation to aid in the design of surfactant formulation. Krafft point—another key property to indicate the solubility of anionic surfactant, was also modeled based on the GC concept. The application of this work was performed through case studies in the fields of enhanced oil recovery, soil remediation and detergency.
Development of in situ gel containing asiaticoside-cyclodextrin complex for periodontal tissue regeneration
Authors:   , Soe Hay
Published: September 2023

Asiaticoside (AS) is active herbal compound isolated from Centella asiatica. It has the potential benefit in promoting type I collagen (COL I) synthesis and osteogenic differentiation in human periodontal ligament cells. However, it has low aqueous solubility which may hamper the bioavailability. Thus, the main objective of this study was to develop the thermoresponsive in situ gels containing AS by using cyclodextrin (CD). To determine the solubility enhancement of AS, phase-solubility profiles were investigated and revealed that CDs enhanced the aqueous solubility of AS. Hydroxypropyl-beta-CD (HPβCD) and sulfobutyl-ether-beta-CD (SBEβCD) were selected due to highly solubilization enhancement of AS. The mucoadhesive polymer i.e., chitosan (CS) or thermogelling agent i.e., poloxamer 407 (P407) was added to investigate the effect of polymer to AS/SBEβCD binary complex. The resulted ternary systems synergistically improved the solubility of AS. Solution-state characterization (1H-NMR) and solid-state characterization (DSC, FT-IR and PXRD) of binary complex (AS/HPβCD or AS/SBEβCD) as well as ternary complex (AS/SBEβCD/CS or AS/SBEβCD/P407) were performed. It indicated that there were some interactions and possibly formed AS/CD inclusion complex. The enhancement of AS dissolution was achieved in both binary and ternary system when compared with intact AS alone. The morphology and particle size were analyzed by TEM and DLS techniques. The large aggregate size provided the evidence that AS solubility was enhanced through the AS/CD aggregate formation or micelle-like behavior. AS loaded nanoparticles were prepared via ionic gelation using anionic SBEβCD and cationic CS. Thermally triggered in situ gels containing AS with the proper SBEβCD/CS weight ratio were developed. The physicochemical and chemical characterizations i.e., appearance, pH, viscosity, gelation temperature, gelation time, in vitro gelling capacity, drug content and entrapment efficiency were within acceptable range. In vitro mucoadhesion and the in vitro release studies revealed that nanoencapsulated in situ gels had excellent mucoadhesive property and performed the sustained release of AS. These formulations somewhat negative effect to ligament cells. However, the CD encapsulated platforms containing AS at low content could express the COL I synthesis. Therefore, it may be promising candidate that can deliver AS for periodontal tissue regeneration.
Protective effects of eriodictyol on hydrogen peroxide-induced cell death in SH-SY5Y cells
Authors:   , Thongin Saowarose
Published: September 2023

Oxidative stress and mitochondrial dysfunction are the major causes of neuronal death in neurodegenerative disorders. Eriodictyol is a flavonoid extracted from the whole plant of Dendrobium ellipsophyllum (thai name: Ueang Thong). The antioxidant and anti-inflammatory effects of eriodictyol have been widely reported.  The present study aimed to investigate the protective effect and mechanisms of eriodictyol on hydrogen peroxide (H2O2)-induced cell death in human neuroblastoma SH-SY5Y cells. The protective effect of eriodictyol on cytotoxicity induced by H2O2 was evaluated using resazurin assay. The effects on intracellular reactive oxygen species (ROS) and mitochondria membrane potential were evaluated by 2´-7’ dichlorodihydrofluorescein diacetate (DCFH-DA) assay and tetramethylrhodamine ethyl ester (TMRE) assay, respectively. The effects of eriodictyol on mitochondria-mediated apoptosis cascade were determined using western blot analysis. The results showed that pretreatment with eriodictyol (0.1, 1 and 10 µM) increased cell viability (p < 0.001), reduced intracellular ROS (p < 0.05) and increased TMRE fluorescent intensity (p < 0.05) compared to H2O2-treated alone. H2O2 induced mitochondria-mediated apoptotic signalling by decreasing Bcl-2/BAX ratio, increasing the expressions of  cytochrome c, caspase-3 and cleaved caspase-3 while eriodictyol pretreatment reduced these events. In conclusion, eriodictyol can protect against H2O2-induced cytotoxicity in SH-SY5Y cells via an antioxidant effect, and consequently inhibit mitochondria-mediated apoptosis cascade.
Anticancer effects of mansonone G derivative on human colorectal cancer cells
Authors:   , Chanvijit Savinee
Published: September 2023

Mansonone G (MG), 1,2-naphthoquinone, isolated from the heartwood of Mansonia gagei Drumm (Chan-Cha-Mod), exhibited several pharmacological effects including anti-bacterial, anti-estrogenic and anti-adipogenic effects. However, anticancer activity of MG and its derivatives on colorectal cancer (CRC) has never been investigated. Therefore, the objective of this study was to investigate the cytotoxic effect of MG and its derivatives and to determine the mechanism(s) underlying cytotoxicity of the most potent MG derivative on two CRC cell lines, HCT-116 cells carrying p53 wild-type and HT-29 cells carrying p53 mutant. In the present study, MG and its derivatives could inhibit viability of HCT-116 and HT-29 cells in a concentration-dependent manner. Of all MG and its derivatives, G07 was the most potent cytotoxic agent toward the cancer cells and less toxic to normal cells, PCS201-010 and CRL-1790. Mechanistic studies revealed that G07 could induce apoptosis through ROS generation in both HCT-116 and HT-29 cells and this effect was abolished by N-acetyl cysteine (NAC). Western blot analysis revealed that G07 downregulated the expression of Bcl-2 and Bcl-xl proteins in both cells and upregulated the expression of Bak protein in HT-29 cells. Moreover, G07 downregulated AKT signaling pathway and modulated ERK1/2 signaling pathway by inhibiting ERK1/2 phosphorylation in HCT-116 cells and activating ERK1/2 phosphorylation in HT-29 cells. Taken together, the present study demonstrated that G07 exerted a potent anticancer effect toward human colorectal cancer cells which was associated with induction of apoptosis, generation of ROS and modulation of ERK1/2 and AKT signaling pathways, suggesting that G07 is a promising compound for further development as an anticancer agent in CRC treatment.
Pharmacokinetic Study Of Cucurbitacin B From Trichosanthes Cucumerina L. In Rats
Authors:   , Hunsakunachai Natthaphon
Published: September 2023

Cucurbitacin B is a triterpenoid, majorly found in Cucurbitaceae family, particularly in the Trichosanthes cucumerina L. fruits. The pharmacological properties of cucurbitacin B have been studied for decades, particularly an anti-tumor and anti-inflammatory activity. However, the pharmacokinetic profile of this compound is still limited and the investigation is needed for further pharmaceutical product development. This study aimed to investigate the pharmacokinetic profile of cucurbitacin B after administering the compound at different doses and routes to the rats. Male Wistar rats, weighed approximately 400-500 g, were treated with cucurbitacin B extracted from Trichosanthes cucumerina L. The cucurbitacin B was administered at 0.1 mg/kg intravenously or at 1, 2, and 4 mg/kg orally. Blood, urine, feces, and internal organs were collected after administration at a designated time. The level of cucurbitacin B in biological samples was determined by liquid chromatography-tandem mass spectrometry. The absolute oral bioavailability of cucurbitacin B was approximately 10%. The maximum concentration in plasma after normalization by dose ranged from 4.85-7.81 µg/L and the time to reach maximum value was approximately within 30 min after oral dosing. The level of cucurbitacin B in plasma increased proportionally to the given dose. After intravenous administration, cucurbitacin B had a large volume of distribution of about 51.65 L/kg and exhibited a high tissue to plasma concentration ratio, approximately 60 to 280-fold in several organs. A negligible amount of unchanged cucurbitacin B could be detected in urine and feces and accounted for less than 1% of the administered dose. Cucurbitacin B had low oral bioavailability but could be distributed extensively into internal organs with a high volume of distribution and tissue to plasma ratio. Only negligible amounts of unchanged cucurbitacin B were excreted via urine and feces suggesting that the compound might be biotransformed before undergoing an excretion. Further studies of the metabolic pathway and tissue uptake mechanism are required to strategize the future development of cucurbitacin B into clinical studies.
Cross-cultural adaptation and psychometric properties of the Thai version of the Patient-Reported Outcomes Measurement Information System-29 (PROMIS-29)
Authors:   , Rawang Polake
Published: September 2023

Abstract 

Purpose    To culturally adapt the Patient-Reported Outcomes Measurement Information System-29 into Thai (T-PROMIS-29) and evaluate the reliability and validity of the culturally adapted questionnaire.

Methods    The translation was performed using the Functional Assessment of Chronic Illness Therapy (FACIT) translation guidelines. Internal consistency and test-retest reliability at a 1-week interval for the translated measure were computed. Construct validity was evaluated by computing correlations between the T-PROMIS-29 scores and selected SF-36 and Fear Avoidance Belief Questionnaire (FABQ) scale scores for both convergent and discriminant validity. 

Results    The study sample comprised of 241 participants with chronic low back pain. Internal consistencies were good to excellent, with Cronbach’s alphas ranging from 0.83 to 0.94. The test-retest reliability of all T-PROMIS-29 domains were moderate to good, with ICCs(2,1) ranging from 0.57 to 0.74. Convergent and discriminant construct validity were satisfactory with moderate correlation coefficients in convergent validity (Spearman correlation coefficients 0.45 to 0.67) and low correlation coefficient in discriminant validity (Spearman correlation coefficients 0.17 to 0.48).    

Conclusions    The findings support the reliability and validity of the T-PROMIS-29 scale scores. The measure can be used to assess key quality of life domains in individuals from Thailand with chronic low back pain.
Application of adversarial networks in search for four top quark production in CMS
Authors:   , Wachirapusitanand Vichayanun
Published: September 2023

One burden of high energy physics data analysis is uncertainty within the measurement, both systematically and statistically. Even with sophisticated neural network techniques that are used to assist in high energy physics measurements, the resulting measurement may suffer from both types of uncertainties. Fortunately, most types of systematic uncertainties are based on knowledge from information such as theoretical assumptions, for which the range and behaviour are known. It has been proposed to mitigate such systematic uncertainties by using a new type of neural network called adversarial neural network (ANN) that would make the discriminator less sensitive to these uncertainties, but this has not yet been demonstrated in a real LHC analysis. This work investigates ANNs using as a benchmark the search for the production of four top quarks, an extremely rare physics process at the LHC and one of the important processes that can prove or disprove the Standard Model. The search for four top quarks in some cases is sensitive to large systematic uncertainties. Discriminators based on traditional and adversarial neural networks are trained and chosen via hyperparameter adjustment. The expected cross section upper limit and expected significance for four top quark production is calculated using traditional neural networks and adversarial neural networks based on simulated proton-proton collisions within the Compact Muon Solenoid detector within Large Hadron Collider, and are compared to existing results. The improvement and further considerations to the search for rare processes at the LHC will be discussed.
Effects Of Organic Molecule Orientations On Perovskite Structure Of Formamidinium Lead Iodide
Authors:   , Sukmas Wiwittawin
Published: September 2023

Latterly, an emergence of the hybrid organic-inorganic perovskites has captivated an increasing rate of world-wide attention due to their approving physical properties. Formamidinium lead iodide (FAPI), a promising compound owing to its high photovoltaic performance, consists of an organic molecule, i.e. the formamidinium (FA) cation, dwelling in the centre of the cubic unit cell, caged by the inorganic framework, PbI6. By adopting the ab initio method based on the density functional theory including the spin-orbit coupling (SOC) effects, the effects of the FA cation on the cubic FAPI were thoroughly and systematically investigated. Solidly armed with Euler’s rotations, energy landscapes responsible for various sets of orientations of the FA cation were evaluated accordingly. From the energy landscapes, the flipping energy barriers are interpreted as thermal agitations needed to flip the FA cation over. The highest energy barrier amongst all those of other orientations is 24.7 meV which is tantamount to T ~ 286 K––the temperature over which the FA molecules randomly reorient. Moreover, it is found that a relatively lowest energy structure when the FA cation is directed along (90°,60°, 45°) direction. Owing to the structural optimisation, the I-Pb-I becomes angled with less than 7°. The H-I distances are optimal and confined only in the shells in accordance with the pair distribution function of the optimal configuration. The resulting configuration additionally breaks the inversion symmetry that leads to the Rashba/Dresselhaus effect within the electronic band structure. The largest Rashba splitting parameter determined along the direction in the k–space is around 3.0 for the (90°,60°, 45°) configuration.
Effects of aldosterone on striatin and caveolin-1 protein levels in rat kidney: role of mineralocorticoid receptor
Authors:   , Inthachart Kevalin
Published: September 2023

The mineralocorticoid hormone aldosterone is a steroid hormone which plays an important role in maintenance of sodium, potassium, and acid-base balance. Aldosterone actions are mediated by mineralocorticoid receptor (MR) that could operate via genomic or nongenomic pathways. It has been shown that MR forms a complex with several molecules including scaffolding proteins, such as striatin and caveolin-1 (cav-1). Previous in vitro studies demonstrated that aldosterone increased striatin and cav-1 protein abundance. In addition, both striatin and cav-1 could interact with MR. However, there is no in vivo study of aldosterone effects on striatin and cav-1 protein levels in rat kidney. Male Wistar rats were divided into two main groups: 30 minutes and 2 hours. Each main group further divided into 3 subgroups: sham (normal saline solution; ip), aldosterone (Aldo: 150 µg/kg BW) or eplerenone [mineralocorticoid receptor (MR) blocker, 15 mg/kg BW] 30 minutes before aldosterone injection. Thirty minutes or two hours after aldosterone administration, protein abundance and localization of striatin and cav-1 were determined by Western blot analysis and immunohistochemistry, respectively. In addition, the protein interaction of striatin/MR, cav-1/MR, and cav-1/striatin was measured by co-immunoprecipitation and Western blot analysis. The results from 30-minute group showed that aldosterone increased protein abundances of striatin and cav-1 to 150% (p<0.05) and 200% (p<0.001), respectively. Eplerenone had no significant effect on striatin levels, whereas cav-1 protein was partially blocked by eplerenone. For 2-hour group, protein abundances of striatin and cav-1 were remained after aldosterone injection. Interestingly, eplerenone pretreatment significantly suppressed cav-1 protein levels. Aldosterone stimulated striatin and cav-1 protein immunoreactivity in both cortex and medulla. Eplerenone minimized striatin and cav-1 immunostaining in the cortex. For protein interaction, the data showed that either striatin or cav-1 was able to interact with MR in rat kidney. This is the first in vivo study demonstrating that, in rat kidney, aldosterone differently modulates striatin and cav-1 protein levels. In a rapid action, aldosterone increases striatin via MR-independent manner, whereas it partially induces cav-1 through MR-dependent pathway. For a longer effect, aldosterone had no significant alterations on striatin and cav-1 protein levels. Blockage of MR could disturb aldosterone actions on cav-1 protein.
Catalytic applications of CuO loaded mesoporous CeO₂ and CuO loaded mesoporous CeO₂-ZrO₂ catalyst
Authors:   , Jampa Sureerat
Published: September 2023

Copper oxide (CuO) loaded mesoporous ceria (CeO₂) and Copper oxide (CuO) loaded mesoporous ceria-zirconia (CeO₂-ZrO₂) catalysts are successfully synthesized for preferential oxidation of CO (CO-PROX) and autothermal steam reforming of methanol (ASRM). Mesoporous CeO₂ and mesoporous CeO₂-ZrO₂ as catalyst supports with high specific surface area and ordered structure are prepared via a nanocasting process by using MCM-48 as template. Deposition-precipitation technique is chosen to load various Cu contents (1 to 9 wt.%) on the synthesized mesoporous supports. The synthesized CuO loaded mesoporous CeO₂ (Cu-C) and CuO loaded mesoporous CeO₂-ZrO₂ (Cu-CZ) catalysts are characterized by N₂ adsorption-desorption, X-ray diffraction, transmission electron microscopy, atomic absorption spectroscopy, X-ray photoelectron spectroscopy, and temperature-programmed reduction by H₂. The catalytic performance of Cu-C and Cu-CZ catalysts for the CO-PROX reaction in presence of excess hydrogen and autothermal steam reforming of methanol (ASRM) reaction are investigated. The Cu-C and Cu-CZ catalysts illustrate an excellent efficiency for these reactions.
Development of composite polyelectrolyte complex membrane for cationic dye and heavy metal sorption
Authors:   , Chueasupcharoen Wanwanut
Published: September 2023

The sorption process is most useful in water treatment because it provides high sorption efficiency, low cost and rapid method. Polyelectrolyte complex (PECs) is a novel polymeric membrane prepared by mixing between oppositely charged polyelectrolyte solution of PDADMAC and PSS. Activated carbon (ACs) is used to improved adsorption efficiency in PECs. Non-stoichiometric PDADMAC/PSS membrane were prepared by mixing excess PSS concentration to get the negative charges on the membrane surface. Sorption efficiency was evaluated by methylene blue, cationic dye and heavy metal ions based on the same material, the difference sorption mechanism were observe. In the cationic dye, the adsorption mechanism operated by excess PSS on membrane surface via electrostatic interaction. The maximum adsorption capacity occur in a removal salt 1:2 PDADMAC:PSS mole ratio (R-1:2) without AC which is 4.9 mg g-1 with 10 mg l-1 of methylene blue concentration. Furthermore, R-1-2 fitted with pseudo-second order kinetic model and Temkin isotherm model with physical adsorption type and also regenerated by salt solution. In contrast, the heavy metal ions sorption operated ion exchange mechanism, 1:2 PDADMAC:PSS without AC show highest sorption capacity which is 30 mg g-1 with 50 mg l -1 of Cu2+ ions concentration. The mechanism occur between Na+ on PSS chain and Cu2+ ions in solution. The metal ions sorption fitted with pseudo-second order kinetic model and Freundlich isotherm model with chemical sorption type and bilayer surface sorption and shows high selective of Pb2+ and Cr 3+metal ions.
Color Stability, Water Sorption And Water Solubility Of Various Artificial Acrylic Resin Teeth
Authors:   , Foungfu Piyaporn
Published: September 2023

Color stability, water sorption and water solubility of the acrylic resin artificial teeth are the success keys to the appearance and mechanical properties of the denture teeth. The objective of the present study was to compare the color stability, water sorption and water solubility of 3 commercial artificial teeth: linear PMMA (NEW), resin composite (CPX) and cross-linked PMMA (ANT) and 1 color-liked heat-polymerized acrylic resin (MAJ). 80 colume-shaped specimens (9 mm/ 2 mm thickness) were fabricated for color stability test using a spectrophotometer after immersing in water or coffee solution at 1, 7, 28, 56 and 84 days. 40 colume-shape specimens ( 9 mm/ 0.5 mm thickness) were fabricated for the water sorption and water solubility tests using the weight measurement method at 1, 8, 36 and 92 days. The repeated 2-way ANOVA was calculated at the confidential level of 95. The results demonstrated the different color change depending on the type of denture teeth (MAJ>NEW>CPX>ANT) from 28 to 84 days, while the color change showed no significant difference at 1 day storage. The water sorption results demonstrated the greatest MAJ, followed by NEW, CPX and ANT respectively at 36 and 92 days, while the water solubility showed the greatest MAJ, followed by CPX, NEW and ANT respectively. This study confirmed the effect of the composition of acrylic resin denture teeth on the color stability, water sorption and water solubility. Moreover, the effect of the fabrication method as well as the type of storage solution was reported.
The efficacy and safety of herbal medicine combination for management of leg symptoms due to chronic venous diseasethai patients : a double blind ed
Authors:   , Udombhornprabha Anan
Published: September 2023

The herbal medicine combination containing 2% Asiaticoside and 1% Acemannan in a 2% Acetyl Salicylic Acid in beeswax encapsulation as an ACTIVE treatment ( HMC) was developed as a topical gel preparation as treatment options for Chronic Venous Disease (CVD) with mild-to-moderate severity. Methods: The efficacy and safety of HMC as compared with a CONTROL treatment (C) (2% Acetylsalicylic Acid) was investigated in a double-blind randomized controlled trial, 42 CVD patients, 21 of whom allocated either HMC or C, for 12-week continuous treatment. The efficacy assessment employed Venous Clinical Severity Score (VCSS), Physician-Rating Symptoms Perception Score (PRSPS), Patients Self-Rating Symptoms Score (PSSS) and two types of short-form health-related quality of life scores, a Medical Assessment Study, 14-item Chronic Venous Disease (MOS CIVIQ 14), and a 12-items Genearal Health (MOS SF 12). The short-term safety assessed with patients-self reported adverse events. The primary endpoint was a responder rate defined by 50% reduction of the VCSS after 4-weeks of treatment from baseline. The secondary endpoints assessed improvement of overall sympotms assessed by VCSS, PRSPS, PSSS, MOS CIVIQ 14 and MOS SF12 accordingly. Results: CVD Thai patients(N= 42) in general and regardless of clinical severity class (CEAP1/ CEAP2) or Comorbidity responded 57.14 % to HMC(N= 21) as compared with 9.52% for C(N= 21), the responder rate was significantly dependent on intervention, p= 0.003, P= 0.003 and p= 0.003 respectively. HMC reduced VCSS significantly better than C, overtime at 4-weeks 11.9048(6.4908) VS 22.4702 (7.9438) p<0.001. HMC reduced PRSPS and PSSS significantly better than C, at Basline to 2-weeks p=0.001 and p=0.017, 2-4 weeks p=0.001 and p= 0.004, and 8-12 weeks p= 0.001 and p= 0.003 respectively. After 12-weeks from baseline, both global and subscore for MOS SF 12 MOS CIVIQ-14, treatment with HMC and C significantly improved at p<0.001, p<0.001 (MOS CIVIQ 14) respectively. For adverse drug events reported, no statistical significant different between HMC and C from baseline to the end of 12-week follow-up (p= 0.756) . Conclusion. The herbal medicine combination containing 2% Asiaticoside, 1% Acemannan in 2% Acetyl Salicylic Acid in Beeswax encapsulation (HMC) as a gel preparation topical administration provided clinical responder rate better than the Control. Both HMC and C were not significant different in terms of safety assessment.
Risk of post-traumatic stress disorder and changing of cardiovascular biomarkers among Bangkok firefighters
Authors:   , Khumtong Chinchuta
Published: September 2023

Urban fireﬁghters are at risk of posttraumatic stress disorder (PTSD) because of their occupational exposure to trauma event. PTSD has been recognized as public health problem because it links to many health consequences including cardiovascular disease. This study was conducted in 2 phases. The first phase; a cross-sectional study, was aimed to determine a prevalence of risk of PTSD among all Bangkok firefighters and to access its associated factors. The second phase; a longitudinal study, aimed to access an association between risk of PTSD and changes of cardiovascular biomarker (Brain Natriuretic Peptide (BNP), Troponin T (TnT), and high-sensitive C-reactive protein (hs-CRP)) after 6-months follow-up. In the first phase of the study, all Bangkok firefighters were recruited for self-reported questionnaire. 302 of 1215 firefighters (24.90%) were met the suggested PCL cut-point criteria for civilian (PCL Scores ≥ 30). Health problems, smoking status, alcohol consumption, physical activity, sleep quality, duration of work, and past exposure to major fire in Thailand were associated with risk of PTSD (p-value<0.05). In phase 2, all firefighters from 2 purposively selected firefighter stations were recruited to participate. Blood check-up was performed in August, 2017 and February, 2018. 19 risk of PTSD and 35 non-risk of PTSD firefighters were participated. Brain-derived neurotrophic factor (BDNF), which is a member of the neurotrophin family of growth factors reflected for long-term memory, was investigated as a biomarker of PTSD. At baseline of study, the results showed a significant different BDNF concentration between risk of PTSD and non-risk of PTSD firefighters. Two cardiovascular biomarkers (hs-CRP and BNP) were associated with PTSD. After 6 month follow-up, 27.8% of firefighters were loss to follow-up and 14 of them were excluded because of blood samples. 12 risk of PTSD and 13 non-risk of PTSD firefighters were completed follow-up. General characteristic of remained firefighters was not different from baseline (p-value>0.05). The results showed that only BNP was associated with risk of PTSD (adjusted OR=31.22). In conclusion, firefighter at risk of PTSD was increased risk of cardiovascular biomarker change. Further intervention and policy related to coping mental health among firefighter should be introduced for reducing the risk of cardiovascular disease.
CO2 reforming of methane on Ni and Co containing γ-Al2O3/HY zeolite supported catalysts
Authors:   , Liu Anchittha
Published: September 2023

The carbon dioxide reforming of methane is one of the most effective processes to produce syngas while reducing greenhouse gases. In this study, the catalytic activity and coke resistance were studied on the Ni-based catalysts with or without Co (10%wt) over γ-Al2O3-HY zeolite support. Such catalysts were prepared by the sol-gel method with various different metal loadings and Co/Ni ratio. The activity tests on the carbon dioxide reforming reaction were performed in a fixed-bed reactor with the CH4:CO2 feed volume ratio of 1:1 under atmospheric pressure at 700°C which is the best temperature for the catalysts activity. It has been found that the addition of Co in the bi-metallic showed better catalytic activity and coking resistivity than the Ni monometallic system suggested by the time-on-stream results and the decrease in the amount of Ni oxide and Co oxide confirmed by the absence of their peaks. Therefore, the good catalyst was 7%Ni-3%Co/γ-Al2O3-HY zeolite that had the highest CO2 and CH4 conversion with highest H2 selectivity.
Factors associated with shift work disorder among nurses in a private hospital Bangkok Thailand
Authors:   , Tangkumnerd Marisa
Published: September 2023

Background : Shift work disorder (SWD) is a kind of circadian rhythms sleep disorder related to sleep problem and shift work schedule. SWD is classified by excessive daytime sleepiness and/or insomnia equal or more than 1 month which is related to shift work schedule. Nurse is a health profession who work as a shift schedule. The aim of this study is to identify associated factors of shift work disorder (SWD) among private hospital nurses.

Method : A questionnaire-based cross-sectional study was conducted among 207 registered nurse in a private hospital in Bangkok, Thailand. Standard questionnaire including insomnia (Bergen Insomnia Scale), fatigue (Fatigue Questionnaire), depression (Patient Health Questionnaire-9) and SWD was administered. The descriptive data were presented by mean and percentage .The prevalence of shift work disorder was calculated. The association between independent variable to SWD were analyzed by Chi-square. Crude and adjusted odd ratio analyses were performed by binary logistic regression to assess the relation.

Results: In this study, mean age of registered nurse were 32.46(± 7.27) years. Most of them were female (94.1 %) and single (71.7%) .The prevalence of SWD among private hospital nurses is 23.9%. In multivariate analyses, we found physical fatigue was increased 3.842 fold-odds of having SWD (AOR = 3.842; 95%CI 1.635, 9.027). Moderate and severe depression and noise exposure during the night were increased 3.224 fold-odds (AOR = 3.224; 95%CI: 1.108, 9.386) and 3.107 fold-odds (AOR = 3.107; 95%CI: 1.261, 7.659) of having SWD respectively. However, insomnia and mental fatigue were not associated with SWD.

Conclusion: One quarter number of nurses in this study reported of SWD and found the factors associated with this were physical fatigue, depression and noise exposure during the night time. Mental health and sleep hygiene management program should consider for enhancing shift work disorder among nurses.
Effectiveness of self-management education through crocheting in improving quality of life and blood glucose level among adult female patients with type 2 diabetes mellitus in public health centers Bangkok Thailand: quasi-experimental study
Authors:   , Nooseisai Monthalee
Published: September 2023

One of Diabetic treatment goal is optimum quality of life. DM patients are suggested to control blood glucose to be in the normal range. Stress may increase blood sugar levels. Diabetes self-management education (DSME) is important for patient’s diabetes management. Outcomes of DSME trend to related with the elements of the DSME interventions. This study aims to assess the effectiveness of self-management education through crocheting program in improving quality of life (QOL) and blood glucose level among adult female patients with type 2 diabetes mellitus in public health centers Bangkok Thailand. A quasi-experimental study with control group was conducted in 2 public health centors. By multistage purposeful sampling technique, 39 adult female patients with type 2 diabetes mellitus were selected from each public health center to be study participants. The education program was implemented for intervention group during September – December 2017 by add up to standard service of public health center, while the control group receive standard care as usual. Descriptive statistics were used to describe participants’ characteristics. The effectiveness of intervention was estimated by regression coefficient of intervention on the change of HbA1c and quality of life. The results indicated that most of participants were housewife (54.5 %), had primary education (79.2 %), had sedentary lifestyle or had some walk (81.8%), were overweight or obese (62.4)%, had moderate stress (64.94 %), had higher awareness and acceptance (55.8% and 50.6%, respectively), had higher knowledge and self-care (67.5% and 51.9%, respectively), and had moderate QOL (64.9%). Average HbA1c (SD) at baseline was 8.26 (.74). After intervention, there were significant change in awareness (p<.001), acceptance (p<.001), QOL (p = .041), and HbA1c (p = .009). To calculate coefficients of intervention on blood glucose level and QOL, hierarchical regression approach was used. For the change of HbA1c, R² was .183, and standardized coefficient of intervention on the change of HbA1c was -.498 (p = .002). For the change of QOL, R² was .054, and standardized coefficient of intervention on the change of HbA1c was .233 (p = .042). The results indicated that, intervention respond around 50% of each unit decrease of HbA1c when influence of other factors was control. The regression equation can explain around 18% of HbA1c variation. For QOL, intervention respond around 23% of each unit increase of QOL when influence of other factors was control, however, this equation can explain around 5% of QOL variation. In conclusion, this self-management education through crocheting program has benefit for female adult patient with type 2 diabetes mellitus in glycemic control and quality of life improvement.
“Krue seumangat; kehamilan sehat tanpa anemia” booklet effect to anemia among pregnant women at Kota Langsa Indonesia : a quasi experimental study
Authors:   , Nahrisah Putri
Published: September 2023

Purpose : To assess the effect of education interventional program by previously developing ane education material (booklet of anemia and supplement reminder) upgrade dietary and iron-folic acid te intake of anemic pregnant women in province Aceh. Patients and methods: It was quasi experimental study using a pre- and posttest design, purposively applied at two municipalities having more than 40% anemic pregnancy. The intervention group (n=70) received two home visits for individual counseling session and was given the paper-based reminder, the control group (n=70) followed the usual antenatal care. The knowledge regard anemia and nutritional anemia score, average daily iron intake according to three days of 24-hours food recall, food frequency questionnaire score of animal and plant iron-rich food, number of iron-folic acid intake, hemoglobin and hematocrit concentration, baby’s birth weight of both groups were measured. A Chi-square, Student’s t-test, Wilcoxon ranked test, Man Whitney U test, Ancova test were used to compare the differences of within and between groups across the time measurements. Results: After the intervention program, there was significant improvement of knowledge score, average daily iron intake (mg/day), food frequency questionnaire score, number of iron-folate intake, hemoglobin and hematocrit concentration in intervention group compared with control group (P<0.005). All anemic pregnancies in intervention group recovered during their third trimester of gestation, differently in the control group where 92.8% anemic pregnancies remained and three babies were delivered with low birth weight. Conclusion: Findings suggest to do education and implementation of reminder were an effective approach to support dietary and iron-folate intake behavior changes.