bluuebunny/crossref_metadata_2025 huggingface dataset:
Fondazione Telethon and Unione Italiana Lotta alla Distrofia Muscolare, a successful partnership for neuromuscular healthcare research of value for patients
Authors: Ambrosini Anna , Baldessari Danila , Pozzi Silvia , Battaglia Manuela , Beltrami Elena , Merico Anna Maria , Rasconi Marco , Monaco Lucia
Published: October 2021

In 2001, Fondazione Telethon and the Italian muscular dystrophy patient organisation Unione Italiana Lotta alla Distrofia Muscolare joined their efforts to design and launch a call for grant applications specifically dedicated to clinical projects in the field of neuromuscular disorders. This strategic initiative, run regularly over the years and still ongoing, aims at supporting research with impact on the daily life of people with a neuromuscular condition and is centred on macro-priorities identified by the patient organisation. It is investigator-driven, and all proposals are peer-reviewed for quality and feasibility. Over the years, this funding program contributed to strengthening the activities of the Italian neuromuscular clinical network, reaching many achievements in healthcare research. Moreover, it has been an enabling factor for innovative therapy experimentation at international level and prepared the clinical ground to make therapies available to Italian patients. The ultimate scope of healthcare research is to ameliorate the delivery of care. In this paper, the achievements of the funded studies are analysed also from this viewpoint, to ascertain to which extent they have fulfilled the original goals established by the patient organisation. The evidence presented indicates that this has been a highly fruitful program. Factors that contributed to its success, lessons learned, challenges, and issues that remain to be addressed are discussed to provide practical examples of an experience that could inspire also other organizations active in the field of rare disease research.
Demographic characteristics, clinical and laboratory features, and the distribution of pathogenic variants in the CFTR gene in the Cypriot cystic fibrosis (CF) population demonstrate the utility of a national CF patient registry
Authors: Yiallouros Panayiotis K. , Matthaiou Andreas Μ. , Anagnostopoulou Pinelopi , Kouis Panayiotis , Libik Malgorzata , Adamidi Tonia , Eleftheriou Adonis , Demetriou Artemios , Ioannou Phivos , Tanteles George A. , Costi Constantina , Fanis Pavlos , Macek Milan , Neocleous Vassos , Phylactou Leonidas A.
Published: October 2021

Background

Specialized clinical care for cystic fibrosis (CF) in Cyprus, a small island country, has been implemented since the 1990s. However, only recently, a national CF patient registry has been established for the systematic recording of patients’ data. In this study, we aim to present data on the epidemiological, genotypic and phenotypic features of CF patients in the country from the most recent data collection in 2019, with particular emphasis on notable rare or unique cases.

Results

Overall, data from 52 patients are presented, 5 of whom have deceased and 13 have been lost to follow-up in previous years. The mean age at diagnosis was 7.2 ± 12.3 years, and the mean age of 34 alive patients by the end of 2019 was 22.6 ± 13.2 years. Patients most commonly presented at diagnosis with acute or persistent respiratory symptoms (46.2%), failure to thrive or malnutrition (40.4%), and dehydration or electrolyte imbalance (32.7%). Sweat chloride levels were diagnostic (above 60 mmol/L) in 81.8% of examined patients. The most common identified mutation was p.Phe508del (F508del) (45.2%), followed by p.Leu346Pro (L346P) (6.7%), a mutation detected solely in individuals of Cypriot descent. The mean BMI and FEV1z-scores were 0.2 ± 1.3 and − 2.1 ± 1.7 across all age groups, respectively, whereas chronicPseudomonas aeruginosacolonization was noted in 26.9% of patients. The majority of patients (74.5%) were eligible to receive at least one of the available CFTR modulator therapies. In 25% of patients we recovered rare or unique genotypic profiles, including the endemic p.Leu346Pro (L346P), the rare CFTR-dup2, the co-segregated c.4200_4201delTG/c.489 + 3A > G, and the polymorphism p.Ser877Ala.

Conclusions

CF patient registries are particularly important in small or isolated populations, such as in Cyprus, with rare or unique disease cases. Their operation is necessary for the optimization of clinical care provided to CF patients, enabling their majority to benefit from evolving advances in precision medicine.
Risk of autoimmune diseases in patients with RASopathies: systematic study of humoral and cellular immunity
Authors: Siano M. A. , Marchetti V. , Pagano S. , Di Candia F. , Alessio M. , De Brasi D. , De Luca A. , Pinna V. , Sestito S. , Concolino D. , Tartaglia M. , Strisciuglio P. , D’Esposito V. , Cabaro S. , Perruolo G. , Formisano P. , Melis D.
Published: October 2021

Background

Abnormalities of the immune system are rarely reported in patients affected by RASopathies. Aim of the current study was to investigate the prevalence of immune system dysfunction in a cohort of patients affected by RASopathies.

Study design

A group of 69 patients was enrolled: 60 at the Federico II University, Naples, 7 at University Magna Graecia of Catanzaro, 2 at “Scuola Medica Salernitana”, Salerno. An age- and sex-matched control group was also enrolled. Autoimmune disorders were investigated according to international consensus criteria. Immune framework was also evaluated by immunoglobulin levels, CD3, CD4, CD8, CD19, CD56 lymphocyte subpopulations, autoantibodies levels and panel of inflammatory molecules, in both patients and controls.

Results

Frequent upper respiratory tract infections were recorded in 2 patients; pneumonia, psoriasis and alopecia in single patients. Low IgA levels were detected in 8/44 patients (18.18%), low CD8 T cells in 13/35 patients (37.14%). Anti-tg and anti-TPO antibodies were detected in 3/24 patients (12.5%), anti r-TSH in 2 cases (8.33%), all in euthyroidism. Serum IgA and CD8 levels were significantly lower in patients than in controls (p 0.00685; p 0.000656 respectively). All tested patients showed increased inflammatory molecules compared to controls. These findings may anticipate the detection of overt autoimmune disease.

Conclusions

Patients affected by RASopathies are at risk to develop autoimmune disorders. Routine screening for autoimmunity is recommended in patients with RASopathy.
Subjective cognitive impairment and presenteeism mediate the associations of rumination with subjective well-being and ill-being in Japanese adult workers from the community
Authors: Toyoshima Kuniyoshi , Ichiki Masahiko , Inoue Takeshi , Shimura Akiyoshi , Masuya Jiro , Fujimura Yota , Higashi Shinji , Kusumi Ichiro
Published: October 2021

Background

In recent years, the roles of rumination, subjective cognitive impairment (SCI), and presenteeism have been emphasized in occupational mental health. However, associations between rumination, SCI, presenteeism, and psychological well-being are not fully understood. We hypothesized that SCI and presenteeism mediate the associations between rumination and subjective well-being (SWB) and subjective ill-being (SIB). Hence, we investigated the mediating roles of SCI and presenteeism in this study.

Methods

A total of 458 adult workers (mean age, 40.8±11.9 years; 44.1% male), who were recruited in Tokyo using convenience sampling, were analyzed in this study. The Ruminative Responses Scale, Cognitive Complaints in Bipolar Disorder Rating Assessment, Work Limitations Questionnaire 8, and Subjective Well-being Inventory were used to evaluate rumination, SCI, presenteeism, and psychological well-being (SWB and SIB), respectively. Path analyses were performed to evaluate the relations between these parameters.

Results

The path analysis indicated that rumination, SCI, and presenteeism were directly and negatively associated with SWB and SIB. Regarding indirect effects, rumination was negatively associated with SWB and SIB via SCI, presenteeism, and both SCI and presenteeism. Furthermore, SCI was negatively associated with SWB and SIB via presenteeism.

Conclusions

The results suggest that SCI and presenteeism mediate the associations of rumination with SWB and SIB in Japanese adult workers. To address the psychological well-being associated with rumination, evaluating SCI and presenteeism simultaneously may be useful in occupational mental health. This study provides key insights into the development of comprehensive intervention strategy based on the biopsychosocial perspective for worker’s psychological well-being.
A retrospective medical chart review of clinical outcomes in children and adolescents with attention-deficit/hyperactivity disorder treated with guanfacine extended-release in routine Canadian clinical practice
Authors: van Stralen Judy , Gill Simerpal K. , Reaume Christopher J. , Handelman Kenneth
Published: October 2021

Objective

This study evaluated clinical outcomes in children and adolescents with attention-deficit/hyperactivity disorder (ADHD) treated with the α2-adrenoceptor agonist guanfacine extended-release (GXR) in routine Canadian clinical practice.

Methods

This retrospective chart review focused on patients with ADHD aged 6–17 years initiating treatment with GXR as monotherapy or adjunctive therapy. Patients were followed for up to 12 months after GXR initiation and, if they had received prior ADHD pharmacotherapy, for 12 months before GXR initiation. The primary outcome was change in ADHD symptoms and functionality based on physician assessments, classified as improvement, no change, or worsening relative to the time of GXR initiation. Treatment-emergent adverse events (TEAEs) were evaluated. Clinical outcomes were also analyzed post hoc according to whether GXR treatment was received as monotherapy or adjunctive therapy, and by select psychiatric comorbidities. Exploratory analyses were conducted in patients who had received prior ADHD pharmacotherapy to evaluate clinical outcomes after initiating GXR.

Results

Improvements in ADHD symptoms were reported for 232/330 (70.3%) patients. Functional improvements in school performance and home life were reported for 213/330 (64.5%) and 209/330 (63.3%) patients, respectively. The most frequent TEAEs (≥ 5%) were somnolence, headache, insomnia, presyncope, and decreased appetite. Improvements in ADHD symptoms were observed when GXR was received as either monotherapy (35/60 [58.3%]) or adjunctive therapy (197/270 [73.0%]). Improvements in ADHD symptoms and functionality were observed in the majority of patients with select psychiatric comorbidities. Among patients who had experienced worsening of symptoms with prior ADHD pharmacotherapy, 44/54 (81.5%) experienced symptom improvement, 33/44 (75.0%) who had previously experienced worsening of school performance improved, and 34/48 (70.8%) who had previously experienced worsening of home life improved.

Conclusion

In Canadian routine clinical practice, most children and adolescents with ADHD treated with GXR experienced improvements in ADHD symptoms and in functionality both at school and at home.
A rare familial rearrangement of chromosomes 9 and 15 associated with intellectual disability: a clinical and molecular study
Authors: Lemskaya Natalya A. , Romanenko Svetlana A. , Rezakova Mariia A. , Filimonova Elena A. , Prokopov Dmitry Yu. , Dolskiy Alexander A. , Perelman Polina L. , Maksimova Yulia V. , Shorina Asia R. , Yudkin Dmitry V.
Published: October 2021

Background

There are many reports on rearrangements occurring separately in the regions of chromosomes 9p and 15q affected in the case under study. 15q duplication syndrome is caused by the presence of at least one extra maternally derived copy of the Prader–Willi/Angelman critical region. Trisomy 9p is the fourth most frequent chromosome anomaly with a clinically recognizable syndrome often accompanied by intellectual disability. Here we report a new case of a patient with maternally derived unique complex sSMC resulting in partial trisomy of both chromosomes 9 and 15 associated with intellectual disability.

Case presentation

We characterise a supernumerary derivative chromosome 15: 47,XY,+der(15)t(9;15)(p21.2;q13.2), likely resulting from 3:1 malsegregation during maternal gametogenesis. Chromosomal analysis showed that a phenotypically normal mother is a carrier of balanced translocation t(9;15)(p21.1;q13.2). Her 7-year-old son showed signs of intellectual disability and a number of physical abnormalities including bilateral cryptorchidism and congenital megaureter. The child’s magnetic resonance imaging showed changes in brain volume and in structural and functional connectivity revealing phenotypic changes caused by the presence of the extra chromosome material, whereas the mother’s brain MRI was normal. Sequence analyses of the microdissected der(15) chromosome detected two breakpoint regions: HSA9:25,928,021-26,157,441 (9p21.2 band) and HSA15:30,552,104-30,765,905 (15q13.2 band). The breakpoint region on chromosome HSA9 is poor in genetic features with several areas of high homology with the breakpoint region on chromosome 15. The breakpoint region on HSA15 is located in the area of a large segmental duplication.

Conclusions

We discuss the case of these phenotypic and brain MRI features in light of reported signatures for 9p partial trisomy and 15 duplication syndromes and analyze how the genomic characteristics of the found breakpoint regions have contributed to the origin of the derivative chromosome. We recommend MRI for all patients with a developmental delay, especially in cases with identified rearrangements, to accumulate more information on brain phenotypes related to chromosomal syndromes.
Association of CaMK2A and MeCP2 signaling pathways with cognitive ability in adolescents
Authors: Lee Li-Ching , Su Ming-Tsan , Huang Hsing-Ying , Cho Ying-Chun , Yeh Ting-Kuang , Chang Chun-Yen
Published: October 2021

The glutamatergic signaling pathway is involved in molecular learning and human cognitive ability. Specific single variants (SNVs, formerly single-nucleotide polymorphisms) in the genes encoding N-methyl-d-aspartate receptor subunits have been associated with neuropsychiatric disorders by altering glutamate transmission. However, these variants associated with cognition and mental activity have rarely been explored in healthy adolescents. In this study, we screened for SNVs in the glutamatergic signaling pathway to identify genetic variants associated with cognitive ability. We found that SNVs in the subunits of ionotropic glutamate receptors, including GRIA1, GRIN1, GRIN2B, GRIN2C, GRIN3A, GRIN3B, and calcium/calmodulin-dependent protein kinase IIα (CaMK2A) are associated with cognitive function. Plasma CaMK2A level was correlated positively with the cognitive ability of Taiwanese senior high school students. We demonstrated that elevating CaMK2A increased its autophosphorylation at T286 and increased the expression of its downstream targets, including GluA1 and phosphor- GluA1 in vivo. Additionally, methyl-CpG binding protein 2 (MeCP2), a downstream target of CaMK2A, was found to activate the expression of CaMK2A, suggesting that MeCP2 and CaMK2A can form a positive feedback loop. In summary, two members of the glutamatergic signaling pathway, CaMK2A and MeCP2, are implicated in the cognitive ability of adolescents; thus, altering the expression of CaMK2A may affect cognitive ability in youth.
Epidemiology and prevention of respiratory syncytial virus infections in children in Italy
Authors: Azzari Chiara , Baraldi Eugenio , Bonanni Paolo , Bozzola Elena , Coscia Alessandra , Lanari Marcello , Manzoni Paolo , Mazzone Teresa , Sandri Fabrizio , Checcucci Lisi Giovanni , Parisi Salvatore , Piacentini Giorgio , Mosca Fabio
Published: October 2021

Respiratory syncytial virus (RSV) is the leading global cause of respiratory infections in infants and the second most frequent cause of death during the first year of life. This highly contagious seasonal virus is responsible for approximately 3 million hospitalizations and 120,000 deaths annually among children under the age of 5 years. Bronchiolitis is the most common severe manifestation; however, RSV infections are associated with an increased long-term risk for recurring wheezing and the development of asthma. There is an unmet need for new agents and a universal strategy to prevent RSV infections starting at the time of birth. RSV is active between November and April in Italy, and prevention strategies must ensure that all neonates and infants under 1 year of age are protected during the endemic season, regardless of gestational age at birth and timing of birth relative to the epidemic season. Approaches under development include maternal vaccines to protect neonates during their first months, monoclonal antibodies to provide immediate protection lasting up to 5 months, and pediatric vaccines for longer-lasting protection. Meanwhile, improvements are needed in infection surveillance and reporting to improve case identification and better characterize seasonal trends in infections along the Italian peninsula. Rapid diagnostic tests and confirmatory laboratory testing should be used for the differential diagnosis of respiratory pathogens in children. Stakeholders and policymakers must develop access pathways once new agents are available to reduce the burden of infections and hospitalizations.
A supervised exercise intervention during cancer treatment for adolescents and young adults—FiGHTING F!T: study protocol of a randomised controlled trial
Authors: Munsie Claire , Ebert Jay , Collins Joanne , Plaster Megan , Joske David , Ackland Timothy
Published: October 2021

Background

High-quality evidence supports the integration of exercise to mitigate treatment-related side effects in a wide range of paediatric and adult cancer cohorts. However, the implementation of exercise in adolescent and young adult (AYA) cancer patients is yet to be explored in depth. FiGHTINGF!T is a randomised controlled cross over trial designed to determine if a supervised, structured, and progressive exercise programme can reduce the decline in physical fitness (V02peak) associated with cancer treatment in AYAs from diagnosis.

Methods/design

A total of 40 AYAs recently diagnosed and due to commence systemic treatment (± 2 weeks) for a primary haematological malignancy or solid tumour will be recruited and randomised to either an immediate exercise intervention or usual care (delayed exercise) for 10 weeks. This randomised controlled crossover trial will see both groups engage in a supervised exercise intervention from either diagnosis (baseline assessment) for 10 weeks (0–10 weeks) or following an interim assessment to 20 weeks (10–20 weeks). The bi-weekly tailored exercise programme will combine aerobic and resistance exercises and be supervised by an Accredited Exercise Physiologist. Participants will complete a range of assessments at 0, 10, and 20 weeks including cardiopulmonary exercise tests, 1 repetition maximum strength measures, physical functioning, and self-reported quality of life measurements. Patient-reported treatment-related toxicities will be recorded on a weekly basis.

Discussion

The FiGHTINGF!T trial will provide insight into the potential benefits of a supervised exercise programme in AYAs undergoing cancer treatment. This trial will contribute to the evidence supporting the necessary integration of exercise during cancer treatment, specifically in the under-reported AYA cohort.

Trial registration

This trial was registered retrospectively with the Australia New Zealand Clinical Trial registry (ACTRN12620000663954). Registered on 10 June 2020
Effectiveness of expiratory technique and induced sputum in obtaining good quality sputum from patients acutely hospitalized with suspected lower respiratory tract infection: a statistical analysis plan for a randomized controlled trial
Authors: Cartuliares Mariana Bichuette , Skjøt-Arkil Helene , Rosenvinge Flemming Schønning , Mogensen Christian Backer , Skovsted Thor Aage , Pedersen Andreas Kristian
Published: October 2021

Background

Targeted antimicrobial treatment is essential to avoid unnecessary use of broad-spectrum antibiotics and antimicrobial resistance. Targeted treatment relies on a precise microbiological diagnosis — in pneumonia, this poses a challenge as the usefulness of Gram stains and cultures is highly dependent on the quality of the sputum sample.

This study aims to examine adverse effects and quality of sputum samples obtained by expiratory techniques (forced expiratory technique and sputum induction) compared with tracheal suction. The hypothesis is that expiratory techniques are non-inferior to tracheal suction in obtaining samples from the lower respiratory tract. This statistical analysis plan (SAP) describes the study design, method, and data analysis of the trial to increase transparency, avoid reporting bias or data-driven analysis and increase the study’s reproducibility.

Method

The design is a pragmatic, non-inferiority, parallel-arm randomized controlled trial including 280 patients admitted with suspected lower respiratory infection to two emergency departments. Patients are randomized to a usual care group, where sputum samples are collected by tracheal suction or to an intervention group where sputum samples are collected by forced expiratory technique and sputum induction. The statistical analysis will follow an intention-to-treat protocol. This SAP is developed and submitted before the end of recruitment, database closure, and statistical analyses.

Discussion

The results of this study will provide valuable knowledge to clinical practice by comparing adverse effects and sputum sample quality associated with different sample methods.

Trial registration

Clinicaltrials.gov, NCT04595526. Submitted on October 19, 2020
Emergence of the invasive malaria vector Anopheles stephensi in Khartoum State, Central Sudan
Authors: Ahmed Ayman , Khogali Rua , Elnour Mohammed-Ahmed B. , Nakao Ryo , Salim Bashir
Published: October 2021

The emergence of the Asian invasive malaria vector, Anopheles stephensi, has been identified in Khartoum, the capital city of Sudan. This is the first report that confirms the geographical expansion of this urban mosquito into Central Sudan. We urgently recommend the launch of a national entomological survey to determine the distribution of this invasive disease vector and to generate essential information about its bionomics and susceptibility to available malaria control measures.

Graphical Abstract
Asthma, allergies and respiratory symptoms in different activity groups of swimmers exercising in swimming halls
Authors: Päivinen Marja , Keskinen Kari , Putus Tuula , Kujala Urho M. , Kalliokoski Pentti , Tikkanen Heikki O.
Published: October 2021

Background

Respiratory symptoms are common in competitive swimmers. However, among these and in swimmers at other activity levels the swimming distance, the total spent time in swimming halls and their medical background varies. Our objectives were, first, to assess their medical histories and the associations with respiratory symptoms among swimmers in different activity groups and then second, to study the pulmonary function findings and related medications in competitive swimmers who exercise in swimming hall environments the most.

Methods

First, 1118 participants consisting of 133 competitive-, 734 fitness- and 251 occasional swimmers answered questionnaires concerning their medical background, their respiratory symptoms in connection to swimming distance and their amount of time spent in swimming halls. Secondly, in 130 competitive swimmers, pulmonary function was tested by spirometry and a specific questionnaire was used to assess respiratory symptoms, medical histories and prescribed medication.

Results

Respiratory symptoms were reported by 18% of the studied swimmers. Competitive swimmers had significantly more symptoms than fitness- and occasional swimmers. Naturally competitive swimmers swum more than 2000 m and stayed by the pool more than 90 min, longer than the other activity groups of swimmers. Spirometry testing showed airway obstruction in 15 swimmers, which was 12% of the 130 competitive swimmers. 21 of them, had physician-diagnosed asthma and 16 of these individuals had prescribed medication for it.

Conclusions

Competitive swimmers had the highest swimming hall exposure and reported significantly more respiratory symptoms. A high prevalence of airway obstruction findings in competitive swimmers with asthma and allergies suggests a need for future recommendations for regular testing and special medical care for competitive swimmers.
Underwater image segmentation in the wild using deep learning
Authors: Drews-Jr Paulo , Souza Isadora de , Maurell Igor P. , Protas Eglen V. , C. Botelho Silvia S.
Published: October 2021

Image segmentation is an important step in many computer vision and image processing algorithms. It is often adopted in tasks such as object detection, classification, and tracking. The segmentation of underwater images is a challenging problem as the water and particles present in the water scatter and absorb the light rays. These effects make the application of traditional segmentation methods cumbersome. Besides that, to use the state-of-the-art segmentation methods to face this problem, which are based on deep learning, an underwater image segmentation dataset must be proposed. So, in this paper, we develop a dataset of real underwater images, and some other combinations using simulated data, to allow the training of two of the best deep learning segmentation architectures, aiming to deal with segmentation of underwater images in the wild. In addition to models trained in these datasets, fine-tuning and image restoration strategies are explored too. To do a more meaningful evaluation, all the models are compared in the testing set of real underwater images. We show that methods obtain impressive results, mainly when trained with our real dataset, comparing with manually segmented ground truth, even using a relatively small number of labeled underwater training images.
Scalable estimator of the diversity for de novo molecular generation resulting in a more robust QM dataset (OD9) and a more efficient molecular optimization
Authors: Leguy Jules , Glavatskikh Marta , Cauchy Thomas , Da Mota Benoit
Published: October 2021

Chemical diversity is one of the key term when dealing with machine learning and molecular generation. This is particularly true for quantum chemical datasets. The composition of which should be done meticulously since the calculation is highly time demanding. Previously we have seen that the most known quantum chemical dataset QM9 lacks chemical diversity. As a consequence, ML models trained on QM9 showed generalizability shortcomings. In this paper we would like to present (i) a fast and generic method to evaluate chemical diversity, (ii) a new quantum chemical dataset of 435k molecules, OD9, that includes QM9 and new molecules generated with a diversity objective, (iii) an analysis of the diversity impact on unconstrained and goal-directed molecular generation on the example of QED optimization. Our innovative approach makes it possible to individually estimate the impact of a solution to the diversity of a set, allowing for effective incremental evaluation. In the first application, we will see how the diversity constraint allows us to generate more than a million of molecules that would efficiently complete the reference datasets. The compounds were calculated with DFT thanks to a collaborative effort through the QuChemPedIA@home BOINC project. With regard to goal-directed molecular generation, getting a high QED score is not complicated, but adding a little diversity can cut the number of calls to the evaluation function by a factor of ten
On-farm colorimetric detection of Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in crude bovine nasal samples
Authors: Pascual-Garrigos Ana , Maruthamuthu Murali Kannan , Ault Aaron , Davidson Josiah Levi , Rudakov Grigorii , Pillai Deepti , Koziol Jennifer , Schoonmaker Jon P. , Johnson Timothy , Verma Mohit S.
Published: October 2021

This work modifies a loop-mediated isothermal amplification (LAMP) assay to detect the bovine respiratory disease (BRD) bacterial pathogens Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in a colorimetric format on a farm. BRD causes a significant health and economic burden worldwide that partially stems from the challenges involved in determining the pathogens causing the disease. Methods such as polymerase chain reaction (PCR) have the potential to identify the causative pathogens but require lab equipment and extensive sample processing making the process lengthy and expensive. To combat this limitation, LAMP allows accurate pathogen detection in unprocessed samples by the naked eye allowing for potentially faster and more precise diagnostics on the farm. The assay developed here offers 66.7–100% analytical sensitivity, and 100% analytical specificity (using contrived samples) while providing 60–100% concordance with PCR results when tested on five steers in a feedlot. The use of a consumer-grade water bath enabled on-farm execution by collecting a nasal swab from cattle and provided a colorimetric result within 60 min. Such an assay holds the potential to provide rapid pen-side diagnostics to cattle producers and veterinarians.
A neural network framework for binary classification of radar detections
Authors: Akhtar Jabran
Published: October 2021

A desired objective in radar target detection is to satisfy two very contradictory requirements: offer a high probability of detection with a low false alarm rate. In this paper, we propose the utilization of artificial neural networks for binary classification of targets detected by a depreciated detection process. It is shown that trained neural networks are capable of identifying false detections with considerable accuracy and can to this extent utilize information present in guard cells and Doppler profiles. This allows for a reduction in the false alarm rate with only moderate loss in the probability of detection. With an appropriately designed neural network, an overall improved system performance can be achieved when compared against traditional constant false alarm rate detectors for the specific trained scenarios.
On local spectral properties of operator matrices
Authors: An Il Ju , Ko Eungil , Lee Ji Eun
Published: October 2021

In this paper, we focus on a$2 \times 2$2×2operator matrix$T_{\epsilon _{k}}$Tϵkas follows:$$\begin{aligned} T_{\epsilon _{k}}= \begin{pmatrix} A & C \\ \epsilon _{k} D & B\end{pmatrix}, \end{aligned}$$Tϵk=(ACϵkDB),where$\epsilon _{k}$ϵkis a positive sequence such that$\lim_{k\rightarrow \infty }\epsilon _{k}=0$limk→∞ϵk=0. We first explore how$T_{\epsilon _{k}}$Tϵkhas several local spectral properties such as the single-valued extension property, the property$(\beta )$(β), and decomposable. We next study the relationship between some spectra of$T_{\epsilon _{k}}$Tϵkand spectra of its diagonal entries, and find some hypotheses by which$T_{\epsilon _{k}}$Tϵksatisfies Weyl’s theorem anda-Weyl’s theorem. Finally, we give some conditions that such an operator matrix$T_{\epsilon _{k}}$Tϵkhas a nontrivial hyperinvariant subspace.
Multiple positive solutions for a system of $(p_{1}, p_{2}, p_{3})$-Laplacian Hadamard fractional order BVP with parameters
Authors: Rao Sabbavarapu Nageswara , Ahmadini Abdullah Ali H.
Published: October 2021

In this article, we are pleased to investigate multiple positive solutions for a system of Hadamard fractional differential equations with $(p_{1}, p_{2}, p_{3})$
(

p
1

,

p
2

,

p
3

)
-Laplacian operator. The main results rely on the standard tools of different fixed point theorems. Finally, we demonstrate the application of the obtained results with the aid of examples.
Exploring carer burden amongst those caring for a child or adolescent with an eating disorder during COVID-19
Authors: Maunder Kristen , McNicholas Fiona
Published: October 2021

Background

Carer burden amongst carers of youth with an eating disorder is substantial and if not addressed can lead to negative outcomes for the patient, carer and family. The Coronavirus Disease 2019 (COVID-19) pandemic has made caring for youth with an ED even more onerous and preliminary research is beginning to emerge demonstrating the profound negative impact the pandemic is having upon individuals with EDs and their carers.

Main

In this review, we briefly summarize what is known about carer burden in families where a young person has an ED, consider the additional impact consequent to COVID-19 and highlight the need for interventions aimed at alleviating this. Pre-COVID-19 research identifies high levels of psychological and physical strain amongst those caring for a child with an ED. Themes are beginning to emerge as to why COVID-19 may further exacerbate carer burden: (1) reduced access to ED services; (2) increased physical vulnerability and exacerbation of psychiatric co-morbidity amongst youth with EDs; (3) increased practical demands placed on carers; and (4) social isolation and decreased social support.

Conclusion

The COVID-19 pandemic poses a specific threat to the mental health of youth with EDs and their carers. Given the salient role families play in caring for youth with an ED, attending to carer burden is imperative. Supporting carers through all phases of their child’s ED journey by offering adaptive and flexible supportive services which accommodate time constraints, geographic barriers and possible COVID-19 spread is essential.
The effects of corticotomy and piezocision in orthodontic canine retraction: a randomized controlled clinical trial
Authors: Fernandes Larissa Salgado da Matta Cid Pinto , Figueiredo Daniel Santos Fonseca , Oliveira Dauro Douglas , Houara Ricardo Gontijo , Rody Wellington José , Gribel Bruno Frazão , Soares Rodrigo Villamarim
Published: October 2021

Background

The aims of this study were to evaluate the efficacy of alveolar corticotomy (AC) and piezocision (PZ) in accelerating maxillary canine retraction, and their effects on multiple bone remodeling expression in gingival crevicular fluid (GCF). A split-mouth, randomized controlled clinical trial was performed at the Department of Orthodontics of Pontifical Catholic University of Minas Gerais, Brazil. Eligibility criteria included orthodontic need for first maxillary premolars extractions, followed by canine retraction. Fifty-one adult patients were recruited and randomly assigned to 3 groups (allocation ratio 1:1:1). Random allocation of surgical or control interventions to each side of the maxillary arch was also conducted: G1 − AC × Control, G2 − PZ × Control, and G3 − AC × PZ. Both the definition of the group and the decision of the experimental or control sides were randomized by the software. Intraoral digital scans were performed before, 7 and 14 days after the beginning of canine retraction, and subsequently, at every 14 days until a maximum period of 6 months. GCF samples were collected before, and 1, 2, 4, 8, and 12 weeks. The primary outcome consisted in the cumulative distal movement of the canines and was measured by digital model superimposition. The secondary outcome consisted in GCF bone remodeling samples that were quantified in a multiplex immunoassay. The measurements examinator was properly blinded.

Results

Forty-seven patients, 19 males and 28 females, were analyzed (mean age 20.72, SD = 6.66, range 15 to 38). Statistically significant differences in canine distal movement between AC and control in G1 were not observed (p > 0.05). In G2, PZ showed lower cumulative incisal and cervical measurements than control from the 2nd to the 24th week (p < 0.05). In G3, PZ showed a lower cumulative incisal and cervical measurements than AC from the16th to the 24th week (p < 0.05). In all groups, differences on biomarkers expression occurred at specific timepoints (p < 0.05), but a distinct pattern was not observed.

Conclusions

AC and PZ were not effective to accelerate maxillary canine retraction and did not induce a distinct pattern of biomarker expression.

Trial registration

NCT03089996. Registered 24 March 2017 - Registered.
Remote sensing detection enhancement
Authors: Ma Tian J.
Published: October 2021

Big Data in the area of Remote Sensing has been growing rapidly. Remote sensors are used in surveillance, security, traffic, environmental monitoring, and autonomous sensing. Real-time detection of small moving targets using a remote sensor is an ongoing, challenging problem. Since the object is located far away from the sensor, the object often appears too small. The object’s signal-to-noise-ratio (SNR) is often very low. Occurrences such as camera motion, moving backgrounds (e.g., rustling leaves), low contrast and resolution of foreground objects makes it difficult to segment out the targeted moving objects of interest. Due to the limited appearance of the target, it is tough to obtain the target’s characteristics such as its shape and texture. Without these characteristics, filtering out false detections can be a difficult task. Detecting these targets, would often require the detector to operate under a low detection threshold. However, lowering the detection threshold could lead to an increase of false alarms. In this paper, the author will introduce a new method that improves the probability to detect low SNR objects, while decreasing the number of false alarms as compared to using the traditional baseline detection technique.
Dynamic response of thickened tailings in shaking table testing
Authors: Alshawmar Fahad , Fall Mamadou
Published: October 2021

In this study, an instrumented thickened tailings deposit model was designed, built and employed to evaluate the behaviour of layered thickened tailings to dynamic loading by using a shaking table equipment. The thickened tailings were deposited subsequently in three thin layers in a flexible laminar shear box mounted on top of the shaking table. Cyclic loading with a peak horizontal acceleration of 0.13 g and a frequency of 1 Hz was applied to the layered tailings deposit. Different types of sensors were placed to monitor the accelerations, displacements, volumetric water content and pore water pressures at the intermediate depth of each layer. Results indicated that the acceleration for the bottom and middle layers were similar to that of the base of the shaking table; but, this was not the case for the top layer. The measurements of vertical displacements indicated that all layers of thickened tailings experienced initially contraction and subsequently dilation during the shaking. The excess pore water pressure ratios were found to exceed unity through all layers of thickened tailings when the shaking ended. In other words, the results showed that the layered thickened tailings are susceptible to liquefaction under the considered testing conditions. It is also found that upward pore water migration to the top layer and downward pore water flow to the bottom layer occurred in the thickened tailings deposit. This water migration generated additional pore water pressure and also impacted the vertical displacement and liquefaction susceptibility of the thickened tailings material. The results of this study give a better understanding of the dynamic behaviour of thickened tailings, which is crucial for the safety of thickened tailings systems as well as sustainable mining.
Implant-abutment screw removal torque values between customized titanium abutment, straight titanium abutment, and hybrid zirconia abutment after a million cyclic loading: an in vitro comparative study
Authors: Klongbunjit Disayut , Aunmeungtong Weerapan , Khongkhunthian Pathawee
Published: October 2021

Purpose

The aim of this study was to compare removal torque values after mechanical cyclic loading and bending moment after the static compression testing of customized titanium abutment compared with prefabricated and hybrid abutments.

Materials and methods

The study was developed according to ISO 14801:2016. Sixty implants were divided into three groups equally: Straight titanium abutment group, Customized titanium abutment group, and Hybrid zirconia abutment group. Abutments were fabricated with zirconia restoration. Forty five implants underwent for cyclic loading. The removal torque values were measured after a fatigue test was conducted at 0 cycles (control), 50,000 cycles and 1,000,000 cycles. In the second experiment, 15 implants were divided into the same groups. Then, bending moments were investigated.

Results

The mean initial removal torque value was significantly higher than 50,000 cycles and 1,000,000 cycles (P < 0.001). The comparison of mean removal torque value between types of abutments was not significantly different (P > 0.05), and the bending moments of all abutments were not significantly different (P > 0.05).

Conclusions

From the boundary of this in-vitro study, it could be concluded that customized titanium abutment and hybrid abutment were not significantly different in terms of removal torque values after the fatigue test. The bending moment between types of abutment were not significantly different. Thus, it could be concluded that abutment type does not significantly influence abutment stability or fracture strength.
The influence of laser-microgrooved implant and abutment surfaces on mean crestal bone levels and peri-implant soft tissue healing: a 3-year longitudinal randomized controlled clinical trial
Authors: Ahamed Assma Syed , Prakash P. S. G. , Crena Jasmine , Victor Dhayanand John , Subramanian Sangeetha , Appukuttan Devapriya
Published: October 2021

Purpose

The study aimed to compare clinical and radiographic parameters of peri-implant site of laser-microgrooved implants with either laser-microgrooved or regular machined prosthetic abutment post 3 years of early loading.

Method

Twenty edentulous sites of systemically and periodontally healthy individuals were allocated into two groups in this prospective, two-armed, randomized double-blinded clinical trial in 1:1 ratio, wherein each site received laser-microgrooved implants with either laser-microgrooved or machined prosthetic abutments. Outcome measures included full-mouth plaque (FMPS%) and bleeding score (FMBS%), site-specific plaque (SPS%) and bleeding score (SBS%), peri-implant sulcus depth (PISD mm), and mean crestal bone loss (MCBL mm) evaluated at baseline (6 weeks), 1 and 3 years post-early loading. Mean and standard deviation of all parameters were estimated, between groups and at different time points using independent and pairedt-test, respectively, Normality was checked using Kolmogorov–Smirnov test and Shapiro–Wilk test,Pvalue ≤ 0.05 was considered as statistically significant.

Results

Three-year follow-up of test group showed statistically significant reduction in SPS, SBS, PISD (Pvalue ≤ 0.001). The MCBL reduced from 1.93 mm to 0.61 mm (Pvalue ≤ 0.001); in other words, a bone gain of 0.15 mm was obtained in the test group.

Conclusion

Within the limitations of the present study, laser-microgrooved implants loaded with laser-microgrooved prosthetic abutments showed superior results clinically and radiographically when compared to loading with machined abutments.
Pharmacokinetics of caffeine self-administered in overdose in a Japanese patient admitted to hospital
Authors: Adachi Koichiro , Beppu Satoru , Terashima Mariko , Fukuda Toshiaki , Tomizawa Jun , Shimizu Makiko , Yamazaki Hiroshi
Published: October 2021

Background

Caffeine (0.1 g) is used as a central nervous system stimulant and as a nontoxic phenotyping probe for cytochrome P450 1A2. However, an increasing number of suicide attempts by caffeine overdose have been recently reported.

Case presentation

A 25-year-old woman (body weight, 43 kg) who intentionally took an overdose of 5.9 g caffeine as a suicide attempt was emergently admitted to Kyoto Medical Center. The plasma concentrations of caffeine and its primary metabolite, N-demethylated paraxanthine, in the current case were 100 and 7.3 μg/mL, 81 and 9.9 μg/mL, 63 and 12 μg/mL, and 21 and 14 μg/mL, at 12, 20, 30, and 56 h after oral overdose, respectively. The observed apparent terminal elimination half-life of caffeine during days 1 and 2 of hospitalization was 27 h, which is several times longer than the reported normal value. This finding implied nonlinearity of caffeine pharmacokinetics over such a wide dose range, which could affect the accuracy of values simulated by a simplified physiologically based pharmacokinetic model founded on a normal dose of 100 mg. Low serum potassium levels (2.9 and 3.5 mM) on days 1 and 2 may have been caused by the caffeine overdose in the current case.

Conclusions

The patient underwent infusion with bicarbonate Ringer’s solution and potassium chloride and was discharged on the third day of hospitalization despite taking a potentially lethal dose of caffeine. The virtual plasma exposures of caffeine estimated using the current simplified PBPK model were higher than the measured values. The present results based on drug monitoring data and additional pharmacokinetic predictions could serve as a useful guide in cases of caffeine overdose.
Entrepreneurship, beekeeping, and health training to decrease community violence in Dar es Salaam, Tanzania: a pilot study for an intervention trial
Authors: Outwater Anne H. , Abraham Alison G. , Iseselo Masunga K. , Sekei Linda Helgesson , Kazaura Method R. , Killewo Japheth
Published: October 2021

Background

High unemployment rates and limited access to resources, services, and economic opportunities are associated with many types of violence. In Dar es Salaam, Tanzania, most violence is experienced by unemployed, poorly educated men between the ages of 20 and 35 years. It is expected that community violence will decrease as the incomes of those most at risk increase. However, economic opportunity through formal employment is rarely available to uneducated men in Dar es Salaam. Giving them access to economic independence through entrepreneurship training is therefore supported by the World Bank and the government of Tanzania. There has been little research on the effectiveness of programs to encourage entrepreneurship.

Methods

To evaluate the feasibility of providing entrepreneurial training programs to young men in Dar es Salaam, especially those without formal employment, a pretest-posttest pilot study was conducted drawing a sample of young men from neighborhood camps called vijiweni. There were four interventions, each implemented in a single camp: Health/Control, Entrepreneurship + Health, Beekeeping + Health, and Entrepreneurship + Beekeeping + Health. The four camps received 2, 6, 6, and 10 training sessions, respectively. No start-up capital was provided. The participants were interviewed at baseline and 3 months, 6 months, and 1 year after the sessions were completed. Data were collected on demographics, household assets, experience of violence, and income.

Results

Fifty-seven respondents attended the first session. At baseline, the camps were not meaningfully different from one another in educational attainment, number of dependents, daily income, assets, or individual members’ roles as victims, perpetrators, or witnesses of violence. Differences were found in age, occupation, and weekly income. Over a period of 2.25 years (from baseline to the end of the project), the weekly income of the Health/Control camp, which had been earning the most, decreased by 37% in a reflection of worsening economic conditions at the time. All three intervention camps increased their income: Beekeeping by 43%, All by 50%, and Entrepreneurship by 146%, with the latter almost reaching the minimum wage level. The most persistently reported constraint was insufficient start-up capital.

Conclusions

The feasibility and potential effectiveness of a short training program on entrepreneurship skills for unemployed, poorly educated young men in urban Tanzania were demonstrated in this study. It has set the stage for an intervention trial to test an updated hypothesis: A 5–7-day intervention about entrepreneurship and microfinance savings groups will lead to increased income and decreased violence.

Trial registration

ClinicalTrials.govNCT 04602416. Registered on 24 October 2020. Retrospectively registered.