bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting the Risk of Malignancy of Lung Nodules Diagnosed as Indeterminate on Radial Endobronchial Ultrasound-Guided Biopsy
Authors: Zo Sungmin , Woo Sook-young , Kim Seonwoo , Lee Jung Eun , Jeong Byeong-Ho , Um Sang-Won , Kim Hojoong , Kwon O Jung , Lee Ho Yun , Lee Kyungjong
Published: November 2020

The next diagnostic step in cases of indeterminate radial probe endobronchial ultrasound (radial EBUS)-guided biopsy results remains uncertain. This study aimed to identify risk factors for malignancy based on clinical findings, chest computed tomography (CT), and radial EBUS images, and to estimate the risk of malignancy in lung nodules that showed indeterminate radial EBUS-guided biopsy results by constructing a nomogram. This retrospective study included 157 patients with indeterminate results on an initial radial EBUS biopsy performed at the Samsung Medical Center from January 2017 to December 2018, but with a definitive final diagnosis. Medical records, chest CT, radial EBUS images, and the final diagnoses were reviewed. Patients were randomly divided into training and validation sets. Factors related to malignancy were identified through logistic regression analysis, and a nomogram was constructed using the training set and subsequently applied to the validation set. Six factors in univariable and multivariable analyses, including upper lobe location, spiculation, satellite nodules, echogenicity, presence of dots or linear arcs, and patency of vessels and bronchi predicted malignancy. A nomogram was constructed based on these predictors. The area under the curve (AUC) value of the nomogram was 0.858 using the chest CT factors, which improved to 0.952 when radial EBUS factors were added. The calibration curve showed good agreement between the actual and nomogram-predicted malignancy outcomes. The utility of radial EBUS images for revealing risk factors of malignancy was confirmed. Furthermore, our nomogram was able to predict the probability of malignancy in lung nodules with indeterminate radial EBUS-guided biopsy results.
Emerging Treatment Options for Gastroenteropancreatic Neuroendocrine Tumors
Authors: Cives Mauro , Pelle’ Eleonora , Strosberg Jonathan
Published: November 2020

Treatment options for neuroendocrine tumors (NETs) and carcinomas (NECs) are expanding. Early-phase studies have shown preliminary evidence of the antitumor activity of alpha-emitting peptide receptor radionuclide therapy (PRRT), and novel radiopeptides incorporating somatostatin receptor antagonists (rather than agonists) have been developed. Several tyrosine kinase inhibitors (TKIs) with antiangiogenic potential have been evaluated in patients with NETs, including lenvatinib, axitinib, cabozantinib and pazopanib. Recently, two phase 3 clinical trials have demonstrated the efficacy and safety of surufatinib, an inhibitor of vascular endothelial growth factor receptor (VEGFR)-1, -2, -3, fibroblast growth factor receptor (FGFR)-1 and colony stimulating factor-1 receptor (CSF-1R), in patients with pancreatic and extra-pancreatic NETs. Multiple clinical trials of combination immunotherapy have been recently completed, but interpretation of the results is hampered by small samples sizes and discordant outcomes. This review summarizes recent data on emerging treatments for neuroendocrine neoplasms.
Lyso-Gb3 Increases αvβ3 Integrin Gene Expression in Cultured Human Podocytes in Fabry Nephropathy
Authors: Trimarchi Hernán , Ortiz Alberto , Sánchez-Niño Maria Dolores
Published: November 2020

Background: Podocyturia in Fabry nephropathy leads to glomerulosclerosis and kidney disease progression. Integrins are involved in podocyte attachment to the glomerular basement membrane. We hypothesized that in Fabry nephropathy, lyso-Gb3 could modulate αvβ3 expression in podocytes. Together with UPAR, the αvβ3 integrin is a key mechanism involved in podocyte detachment and podocyturia. Methods: In cultured human podocytes stimulated with lyso-Gb3, the mRNA expression of the ITGAV and ITGB3 genes encoding integrins αv and β3, respectively, was analyzed by RT-qPCR. Results: In cultured human podocytes, lyso-Gb3 at concentrations encountered in the serum of Fabry patients increased ITGAV and ITGB3 mRNA levels within 3 to 6 h. This pattern of gene expression is similar to that previously observed for PLAUR (UPAR) gene expression but is in contrast to the delayed (24 h) upregulation of other markers of podocyte stress and mediators of injury, such as CD80, TGFβ1, CD74, Notch1, and HES. Conclusions: Human podocyte stress in response to glycolipid overload in Fabry nephropathy, exemplified by lyso-Gb3, is characterized by an early increase in the expression of components of the αvβ3/UPAR system, which contrasts with the delayed rise in the expression of other mediators of podocyte injury. This suggests that the αvβ3/UPAR system may be a therapeutic target in Fabry nephropathy.
Twin Marine Hydrokinetic Cross-Flow Turbines in Counter Rotating Configurations: A Laboratory-Scaled Apparatus for Power Measurement
Authors: Doan Minh N. , Kai Yuriko , Obi Shinnosuke
Published: November 2020

This article proposes an experimental apparatus design to measure the power of a cross-flow marine hydrokinetic turbine system operating in a laboratory water tunnel. Data, from one Hall sensor output signal, was processed to capture the three types of torque exerted on the turbines: mechanical loss, brake, and hydrodynamic torque. The method was then applied to compare the power of a twin turbine system in different counter-rotating configurations. Controlled by a hysteresis brake, the tip-speed-ratio was varied in a constant freestream velocity of 0.316 m/s. While the braking torque was independent of the speed, the mechanical loss was found to depend on the system rotational speed and the amount of mass mounted on the mechanical support. In a counter-rotating configuration, the turbines were synchronized through a pair of spur gears and timing pulleys. Operating at the average chord based Reynolds number of 8000, each turbine had three NACA0012 blades mounted at 15∘ pitch angle. The power coefficient results of 8 turbine configurations showed the tendency of power enhancement of counter-rotating configurations due to blade interaction and increase in blockage ratio. Comparison of the results suggested direct application in a river flow scenario and manipulation of the blade interaction for optimal power production.
Is the Frequency of Candidemia Increasing in COVID-19 Patients Receiving Corticosteroids?
Authors: Riche Cezar V. W. , Cassol Renato , Pasqualotto Alessandro C.
Published: November 2020

Corticosteroids have potent anti-inflammatory and immunosuppressive effects. Recently, these medications have gained importance in the treatment of severe COVID-19. Here we present data demonstrating a marked (10-fold) increase in frequency of candidemia in hospitalized patients with COVID-19 receiving corticosteroids in Brazil. Overall mortality was 72.7%, despite antifungal therapy. Physicians should be aware of the potential risk for candidemia among severely ill COVID-19 patients receiving high-doses of corticosteroids.
Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by Radiotherapy
Authors: Wojakowska Anna , Zebrowska Aneta , Skowronek Agata , Rutkowski Tomasz , Polanski Krzysztof , Widlak Piotr , Marczak Lukasz , Pietrowska Monika
Published: November 2020

Background: In general, the serum metabolome reflects the patient’s body response to both disease state and implemented treatment. Though serum-derived exosomes are an emerging type of liquid biopsy, the metabolite content of these vesicles remains under researched. The aim of this pilot study was to compare the metabolite profiles of the whole serum and serum-derived exosomes in the context of differences between cancer patients and healthy controls as well as patients’ response to radiotherapy (RT). Methods: Serum samples were collected from 10 healthy volunteers and 10 patients with head and neck cancer before and after RT. Metabolites extracted from serum and exosomes were analyzed by the gas chromatography–mass spectrometry (GC–MS). Results: An untargeted GC–MS-based approach identified 182 and 46 metabolites in serum and exosomes, respectively. Metabolites that differentiated cancer and control samples, either serum or exosomes, were associated with energy metabolism. Serum metabolites affected by RT were associated with the metabolism of amino acids, sugars, lipids, and nucleotides. Conclusions: cancer-related features of energy metabolism could be detected in both types of specimens. On the other hand, in contrast to RT-induced changes observed in serum metabolome, this pilot study did not reveal a specific radiation-related pattern of exosome metabolites.
Effects of IPO Offer Price Ranges on Initial Subscription, Initial Turnover and Ownership Structure—Evidence from Indian IPO Market
Authors: Sandhu Harsimran , Guhathakurta Kousik
Published: November 2020

In this paper, we establish the significance and effects of initial public offer (IPO) offer price ranges on subscription, initial trading, and post-IPO ownership structures. The primary market in India provides a unique setting for estimating the effect of various initial public offer (IPO) price ranges and IPO issue factors on the initial demand for an IPO among investors, measured by full IPO subscription/oversubscription, initial turnover (liquidity), and the post-IPO listing ownership structure among investors (ownership). For the IPO pre-listing stage, this study uses firth logistic regression to estimate the effect of various IPO offer price ranges (low to high) and various IPO issue factors on the full subscription/oversubscription of an IPO in each investor category. For the post-IPO listing stage, the study uses OLS regression to estimate the effect of various IPO offer price ranges (low to high) and various IPO issue factors on the initial trading ratio (IPO listing day trading) and the ownership percentage between institutional and individual investors. We find that all investor categories show a lesser likelihood for full subscription or oversubscription of an IPO issue at the lowest range of IPO offer prices. At the post-listing stage, the results indicate a diverse IPO offer price range in which individuals and institutions maximize their respective ownership holdings after the IPO listing. The results further show that lower promoter holdings diffuse higher ownership among individual shareholders by targeting lower IPO offer prices, thus increasing control.
A New Application for the Goal Programming—The Target Decision Rule for Uncertain Problems
Authors: Gaspars-Wieloch Helena
Published: November 2020

The goal programming (GP) is a well-known approach applied to multi-criteria decision making (M-DM). It has been used in many domains and the literature offers diverse extensions of this procedure. On the other hand, so far, some evident analogies between M-DM under certainty and scenario-based one-criterion decision making under uncertainty (1-DMU) have not been revealed in the literature. These similarities give the possibility to adjust the goal programming to an entirely new domain. The purpose of the paper is to create a novel method for uncertain problems on the basis of the GP ideas. In order to achieve this aim we carefully examine the analogies occurring between the structures of both issues (M-DM and 1-DMU). We also analyze some differences resulting from a different interpretation of the data. By analogy to the goal programming, four hybrids for 1-DMU are formulated. They differ from each other in terms of the type of the decision maker considered (pessimist, optimist, moderate). The new decision rule may be helpful when solving uncertain problems since it is especially designed for neutral criteria, which are not taken into account in existing procedures developed for 1-DMU.
Development of Scheelite Tailings-Based Ceramic Formulations with the Potential to Manufacture Porcelain Tiles, Semi-Stoneware and Stoneware
Authors: Figueirêdo Julliana Marques R. de , Costa Fabiana Pereira da , Fernandes Jucielle Veras , Rodrigues Alisson Mendes , Neves Gelmires de Araújo , Menezes Romualdo Rodrigues , Santana Lisiane Navarro de Lima
Published: November 2020

New ceramic formulations based on scheelite tailing were developed, and their potential in the ceramic industry was evaluated. Green bodies with different contents of scheelite tailing (0–8 wt%) were sintered (1150 °C, 1200 °C, and 1250 °C) and characterized in terms of the main mineralogical phases, microstructure, and physico-mechanical properties. The mullite was the main phase identified in all sintered temperatures. This result was also ratified with the aid of scanning electron microscope (SEM) images, in which small needles of the mullite were detected. The presence of mullite is required because it contributes to increasing the mechanical resistance of the material. The physico-mechanical properties measured (water absorption, linear shrinkage, apparent porosity, and flexural strength) were compared to the ISO 13006, and the samples sintered at 1150 °C presented potential to be used as semi-stoneware, while those sintered at 1200 °C and 1250 °C can be employed stoneware and porcelain tiles, respectively.
An A- and B-Site Substitutional Study of SrFeO3−δ Perovskites for Solar Thermochemical Air Separation
Authors: Farr Tyler P. , Nguyen Nhu Pailes , Bush H. Evan , Ambrosini Andrea , Loutzenhiser Peter G.
Published: November 2020

An A‑ and B‑site substitutional study of SrFeO3−δ perovskites (A’xA1−xB’yB1−yO3−δ, where A = Sr and B = Fe) was performed for a two‑step solar thermochemical air separation cycle. The cycle steps encompass (1) the thermal reduction of A’xSr1−xB’yFe1−yO3−δ driven by concentrated solar irradiation and (2) the oxidation of A’xSr1−xB’yFe1−yO3−δ in air to remove O2, leaving N2. The oxidized A’xSr1−xB’yFe1−yO3−δ is recycled back to the first step to complete the cycle, resulting in the separation of N2 from air and concentrated solar irradiation. A-site substitution fractions between 0 ≤ x ≤ 0.2 were examined for A’ = Ba, Ca, and La. B-site substitution fractions between 0 ≤ y ≤ 0.2 were examined for B’ = Cr, Cu, Co, and Mn. Samples were prepared with a modified Pechini method and characterized with X-ray diffractometry. The mass changes and deviations from stoichiometry were evaluated with thermogravimetry in three screenings with temperature- and O2 pressure-swings between 573 and 1473 K and 20% O2/Ar and 100% Ar at 1 bar, respectively. A’ = Ba or La and B’ = Co resulted in the most improved redox capacities amongst temperature- and O2 pressure-swing experiments.
Are Single Polymer Network Hydrogels with Chemical and Physical Cross-Links a Promising Dynamic Vibration Absorber Material? A Simulation Model Inquiry
Authors: Kari Leif
Published: November 2020

Tough, doubly cross-linked, single polymer network hydrogels with both chemical and physical cross-links display a high loss factor of the shear modulus over a broad frequency range. Physically, the high loss factor is resulting from the intensive adhesion–deadhesion activities of the physical cross-links. A high loss factor is frequently required by the optimization processes for optimal performance of a primary vibration system while adopting a dynamic vibration absorber, in particular while selecting a larger dynamic vibration absorber mass in order to avoid an excess displacement amplitude of the dynamic vibration absorber springs. The novel idea in this paper is to apply this tough polymer hydrogel as a dynamic vibration absorber spring material. To this end, a simulation model is developed while including a suitable constitutive viscoelastic material model for doubly cross-linked, single polymer network polyvinyl alcohol hydrogels with both chemical and physical cross-links. It is shown that the studied dynamic vibration absorber significantly reduces the vibrations of the primary vibration system while displaying a smooth frequency dependence over a broad frequency range, thus showing a distinguished potential for the tough hydrogels to serve as a trial material in the dynamic vibration absorbers in addition to their normal usage in tissue engineering.
An Efficient Framework for Adequacy Evaluation through Extraction of Rare Load Curtailment Events in Composite Power Systems
Authors: Abdel Menaem Amir , Valiev Rustam , Oboskalov Vladislav , Hassan Taher S. , Rezk Hegazy , Ibrahim Mohamed N.
Published: November 2020

With the growing robustness of modern power systems, the occurrence of load curtailment events is becoming lower. Hence, the simulation of these events constitutes a challenge in adequacy indices assessment. Due to the rarity of the load curtailment events, the standard Monte Carlo simulation (MCS) estimator of adequacy indices is not practical. Therefore, a framework based on the enhanced cross-entropy-based importance sampling (ECE-IS) method is introduced in this paper for computing the adequacy indices. The framework comprises two stages. Using the proposed ECE-IS method, the first stage’s purpose is to identify the samples or states of the nodal generation and load that are greatly significant to the adequacy indices estimators. In the second stage, the density of the input variables’ conditional on the load curtailment domain obtained by the first stage are used to compute the nodal and system adequacy indices. The performance of the ECE-IS method is verified through a comparison with the standard MCS method and the recent techniques of rare events simulation in literature. The results confirm that the proposed method develops an accurate estimation for the nodal and system adequacy indices (loss of load probability (LOLP), expected power not supplied (EPNS)) with appropriate convergence value and low computation time.
Design of Fractional Order Controllers Using the PM Diagram
Authors: Garrido Santiago , Monje Concepción A. , Martín Fernando , Moreno Luis
Published: November 2020

This work presents a modeling and controller tuning method for non-rational systems. First, a graphical tool is proposed where transfer functions are represented in a four-dimensional space. The magnitude is represented in decibels as the third dimension and a color code is applied to represent the phase in a fourth dimension. This tool, which is called Phase Magnitude (PM) diagram, allows the user to visually obtain the phase and the magnitude that have to be added to a system to meet some control design specifications. The application of the PM diagram to systems with non-rational transfer functions is discussed in this paper. A fractional order Proportional Integral Derivative (PID) controller is computed to control different non-rational systems. The tuning method, based on evolutionary computation concepts, relies on a cost function that defines the behavior in the frequency domain. The cost value is read in the PM diagram to estimate the optimum controller. To validate the contribution of this research, four different non-rational reference systems have been considered. The method proposed here contributes first to a simpler and graphical modeling of these complex systems, and second to provide an effective tool to face the unsolved control problem of these systems.
Intrinsic Discontinuities in Solutions of Evolution Equations Involving Fractional Caputo–Fabrizio and Atangana–Baleanu Operators
Authors: Angstmann Christopher Nicholas , Jacobs Byron Alexander , Henry Bruce Ian , Xu Zhuang
Published: November 2020

There has been considerable recent interest in certain integral transform operators with non-singular kernels and their ability to be considered as fractional derivatives. Two such operators are the Caputo–Fabrizio operator and the Atangana–Baleanu operator. Here we present solutions to simple initial value problems involving these two operators and show that, apart from some special cases, the solutions have an intrinsic discontinuity at the origin. The intrinsic nature of the discontinuity in the solution raises concerns about using such operators in modelling. Solutions to initial value problems involving the traditional Caputo operator, which has a singularity inits kernel, do not have these intrinsic discontinuities.
Vertical Bone Gain after Sinus Lift Procedures with Beta-Tricalcium Phosphate and Simultaneous Implant Placement—A Cross-Sectional Study
Authors: Aragoneses Lamas Juan Manuel , Gómez Sánchez Margarita , Cuadrado González Leví , Suárez García Ana , Aragoneses Sánchez Javier
Published: November 2020

Objectives: The objective of this cross-sectional study was to evaluate the vertical bone gain achieved after the sinus lift procedure with beta-tricalcium phosphate (β-TCP) used as a bone substitute and simultaneous implant placement. Methods: One hundred and twenty-eight sinus lift procedures (utilizing a synthetic ceramic containing 99.9% tricalcium phosphate as a bone substitute) and simultaneous implant placements were performed on 119 patients. The lateral window approach surgical protocol for maxillary sinus lift was performed on the patients. The implants were evaluated using cone-beam computed tomography (CBCT) at 6 months following placement. The vertical bone gain was considered a primary variable, while implant length, diameter, and location were considered secondary variables. Results: The ANOVA results showed no statistical difference in vertical bone gain with implant parameters like implant length, width, and position. Pearsons correlation revealed a statistically significant positive correlation with vertical bone gain and implant length and diameter. A further multivariate linear regression analysis was performed and it observed statistically significant associations between the variables in the study after adjusting for confounding factors. Conclusions: This study concluded that there was vertical bone gain with the usage of β-TCP in maxillary sinus lift surgical procedure with immediate implant placement and that implant variables like length and diameter had a significant influence on the average bone gain values. The implant position did not have a statistically significant influence but there was considerable variation in the bone gain between first, second premolar, and molar regions.
Theoretical Analysis of the Stationary Transport of 1:1 Salt Ions in a Cross-Section of a Desalination Channel, Taking into Account the Non-Catalytic Dissociation/Recombination Reaction of Water Molecules
Authors: Urtenov Makhamet , Gudza Vitaly , Chubyr Natalia , Shkorkina Inna
Published: November 2020

In electromembrane systems, the theoretical study of salt ion transport usually uses mathematical models of salt ion transport in the depleted diffusion layer of ion-exchange membranes. This study uses a one-dimensional mathematical model of salt ion transport in a cross-section of a desalination channel formed by anion-exchange and cation-exchange membranes, taking into account an effect of a dissociation/recombination reaction of water molecules. The reaction on the one hand leads to an overlimiting mass transfer due to the effect of exaltation of the limiting current. On the other hand, an appearance of new electric charge carriers (hydrogen and hydroxyl ions) can reduce the space charge that occurs in membranes and suppress an electroconvective mechanism of overlimiting transport. Thus, there is a problem of studying these phenomena together, taking into account their mutual influence, and this article is devoted to the solution of this problem. Theoretically, using a method of mathematical modeling and numerical research, main regularities are established; in particular, it is shown that the dissociation/recombination reaction of water molecules does not lead to the destruction of the double electric layer at the membranes, but also creates a new double electric layer in the middle of the desalination channel. Thus, the space charge and the dissociation/recombination reaction significantly affect each other and simultaneously the transport of salt ions.
Fatigue Behavior of Conventional and Stationary Shoulder Friction Stir Welded EN AW-5754 Aluminum Alloy Using Load Increase Method
Authors: Baqerzadeh Chehreh Abootorab , Grätzel Michael , Bergmann Jean Pierre , Walther Frank
Published: November 2020

The load increase method, which is highly efficient in rapidly identifying the fatigue performance and strength of materials, is used in this study to investigate friction stir welded (FSW) EN AW-5754 aluminum alloys. Previous investigations have demonstrated the accuracy and efficiency of this method compared to Woehler tests. In this study, it is shown that the load increase method is a valid, accurate and efficient method for describing the fatigue behavior of FSW weld seams. The specimen tests were performed on 2 mm thick aluminum sheets using conventional and stationary tool configurations. It is shown that an increase in fatigue strength of the FSW EN AW-5754 aluminum alloys can be achieved by using the stationary shoulder tool configuration rather than the conventional one.
Recycling Potential of Lithium–Sulfur Batteries—A First Concept Using Thermal and Hydrometallurgical Methods
Authors: Schwich Lilian , Sabarny Paul , Friedrich Bernd
Published: November 2020

High-energy battery systems are gaining attention in the frame of global demands for electronic devices and vehicle electrification. This context leads to higher demands in terms of battery system properties, such as cycle stability and energy density. Here, Lithium–Sulfur (Li–S) batteries comprise an alternative to conventional Li-Ion battery (LIB) systems and can be asserted to next-generation electric storage systems. They offer a promising solution for contemporary needs, especially for applications requiring a higher energy density. In a global environment with increasing sustainable economics and ambitions towards commodity recirculation, the establishing of new technologies should also be evaluated in terms of their recycling potential. In this sense, innovative recycling considers highly valuable metals but also mobilizes all technologically relevant materials for reaching a high Recycling Efficiency (RE). This study uses an approach in which the recycling of Li–S batteries is addressed. For this purpose, a holistic recycling process using both thermal and hydrometallurgical steps is suggested for a safe treatment in combination with a maximum possible recycling efficiency. According to the batteries’ chemical composition, the containing elements are recovered separately, while a multi-step treatment is chosen. Hence, a thermal treatment in combination with a subsequent mechanical comminution separates a black mass powder containing all recoverable resources from the metal casing. The black mass is then treated further in an aqueous solution using different solid/liquid ratios: 1:20, 1:50, 1:55, and 1:100. Different basic and acidic leaching solutions are compared with one another: sulfuric acid (H2SO4), nitric acid (HNO3), hydrochloric acid (HCl), and NaOH. For further precipitation steps, different additives for a pH adjustment are also contrasted: sodium hydroxide (NaOH) and potassium hydroxide (KOH). The results are evaluated by both purity and yield; chemical analysis is performed by ICP-OES (inductively coupled plasma optical emission spectrometry). The aim of this recycling process comprises a maximum yield for the main Li–S battery fractions: Li, S, C, and Al. The focal point for the evaluation comprises lithium yields, and up to 93% of lithium could be transferred to a solid lithium carbonate product.
Towards a More Reliable Identification of Isomeric Metabolites Using Pattern Guided Retention Validation
Authors: Opialla Tobias , Kempa Stefan , Pietzke Matthias
Published: November 2020

Reliable analyte identification is critical in metabolomics experiments to ensure proper interpretation of data. Due to chemical similarity of metabolites (as isobars and isomers) identification by mass spectrometry or chromatography alone can be difficult. Here we show that isomeric compounds are quite common in the metabolic space as given in common metabolite databases. Further, we show that retention information can shift dramatically between different experiments decreasing the value of external or even in-house compound databases. As a consequence the retention information in compound databases should be updated regularly, to allow a reliable identification. To do so we present a feasible and budget conscious method to guarantee updates of retention information on a regular basis using well designed compound mixtures. For this we combine compounds in “Ident-Mixes”, showing a way to distinctly identify chemically similar compounds through combinatorics and principle of exclusion. We illustrate the feasibility of this approach by comparing Gas chromatography (GC)–columns with identical properties from three different vendors and by creating a compound database from measuring these mixtures by Liquid chromatography–mass spectrometry (LC–MS). The results show the high influence of used materials on retention behavior and the ability of our approach to generate high quality identifications in a short time.
Corynebacterium matruchotii Demography and Adhesion Determinants in the Oral Cavity of Healthy Individuals
Authors: Esberg Anders , Barone Angela , Eriksson Linda , Lif Holgerson Pernilla , Teneberg Susann , Johansson Ingegerd
Published: November 2020

Corynebacterium matruchotii may be key in tooth biofilm formation, but information about demographics, bacterial partners, and binding ligands is limited. The aims of this study were to explore C. matruchotii’s demography by age and colonization site (plaque and saliva), in vitro bacterial–bacterial interactions in coaggregation and coadhesion assays, and glycolipids as potential binding ligands in thin-layer chromatogram binding assays. C. matruchotii prevalence increased from 3 months to 18 years old, with 90% and 100% prevalence in saliva and tooth biofilm, respectively. C. matruchotii aggregated in saliva in a dose-dependent manner but lacked the ability to bind to saliva-coated hydroxyapatite. In vivo, C. matruchotii abundance paralleled that of Actinomyces naeslundii, Capnocytophaga sp. HMT 326, Fusobacterium nucleatum subsp. polymorphum, and Tannerella sp. HMT 286. In vitro, C. matruchotii bound both planktonic and surface-bound A. naeslundii, Actinomyces odontolyticus, and F. nucleatum. In addition, C. matruchotii exhibited the ability to bind glycolipids isolated from human erythrocytes (blood group O), human granulocytes, rabbit intestine, human meconium, and rat intestine. Binding assays identified candidate carbohydrate ligands as isoglobotriaosylceramide, Galα3-isoglobotriaosylceramide, lactotriaosylceramide, lactotetraosylceramide, neolactotetraosylceramide, and neolactohexaosylceramide. Thus, C. matruchotii likely uses specific plaque bacteria to adhere to the biofilm and may interact with human tissues through carbohydrate interactions.
Bioleaching of Iron, Copper, Lead, and Zinc from the Sludge Mining Sediment at Different Particle Sizes, pH, and Pulp Density Using Acidithiobacillus ferrooxidans
Authors: Rouchalova Dana , Rouchalova Kamila , Janakova Iva , Cablik Vladimir , Janstova Sarah
Published: November 2020

Globally, the amounts of metal ore deposits have been declining, so the research directions investigating the extraction of metals from materials that are classified as waste are gaining more importance every year. High concentrations of Cu, Pb, Zn, and Fe were analyzed in the sludge sediment (Zlaté Hory, Czech Republic), which is a waste product of the mining industry. In the bioleaching process, bacterial cells have been established as being able to convert metals from solid to liquid phase. However, the most important parameters of bioleaching are particle size, pH, and pulp density, thus our research focused on their optimization. The acidophilic and mesophilic bacteria Acidithiobacillus ferrooxidans were applied due to the high Fe content in the sample. The recovery of metals in the leachate was determined by F-AAS and the residual metal concentrations in the waste fraction were analyzed by XRF. The grain size fractions <40 µm –200 µm were investigated. The atomic absorption spectrometry (AAS) results show that the highest Fe (76.48%), Cu (82.01%), and Pb (88.90%) recoveries were obtained at particle size of 71–100 μm. Zn was dissolved for all fractions above 90%. Experiments with different pH values were performed at a pH of 1.6–2.0. The highest dissolution rates of Zn, Fe, and Cu were achieved with a suspension pH of 1.8, where 98.73% of Zn, 85.42% of Fe, and 96.44% of Cu were recovered. Due to the high percentage dissolution of metals, experiments were performed under pilot conditions in a bioreactor at a pulp density of 2.5% and 4.2% (w/v). From an economic point of view, the leaching time of 28 days was evaluated as sufficient.
Imaging and Characterization of Sustained Gadolinium Nanoparticle Release from Next Generation Radiotherapy Biomaterial
Authors: Mueller Romy , Moreau Michele , Yasmin-Karim Sayeda , Protti Andrea , Tillement Olivier , Berbeco Ross , Hesser Jürgen , Ngwa Wilfred
Published: November 2020

Smart radiotherapy biomaterials (SRBs) present a new opportunity to enhance image-guided radiotherapy while replacing routinely used inert radiotherapy biomaterials like fiducials. In this study the potential of SRBs loaded with gadolinium-based nanoparticles (GdNPs) is investigated for magnetic resonance imaging (MRI) contrast. GdNP release from SRB is quantified and modelled for accurate prediction. SRBs were manufactured similar to fiducials, with a cylindrical shell consisting of poly(lactic-co-glycolic) acid (PLGA) and a core loaded with GdNPs. Magnetic resonance imaging (MRI) contrast was investigated at 7T in vitro (in agar) and in vivo in subcutaneous tumors grown with the LLC1 lung cancer cell line in C57/BL6 mice. GdNPs were quantified in-phantom and in tumor and their release was modelled by the Weibull distribution. Gd concentration was linearly fitted to the R1 relaxation rate with a detection limit of 0.004 mmol/L and high confidence level (R2 = 0.9843). GdNP loaded SRBs in tumor were clearly visible up to at least 14 days post-implantation. Signal decrease during this time showed GdNP release in vivo, which was calculated as 3.86 ± 0.34 µg GdNPs release into the tumor. This study demonstrates potential and feasibility for SRBs with MRI-contrast, and sensitive GdNP quantification and release from SRBs in a preclinical animal model. The feasibility of monitoring nanoparticle (NP) concentration during treatment, allowing dynamic quantitative treatment planning, is also discussed.
Nanomaterial-Based CO2 Sensors
Authors: Rezk Marwan Y. , Sharma Jyotsna , Gartia Manas Ranjan
Published: November 2020

The detection of carbon dioxide (CO2) is critical for environmental monitoring, chemical safety control, and many industrial applications. The manifold application fields as well as the huge range of CO2 concentration to be measured make CO2 sensing a challenging task. Thus, the ability to reliably and quantitatively detect carbon dioxide requires vastly improved materials and approaches that can work under different environmental conditions. Due to their unique favorable chemical, optical, physical, and electrical properties, nanomaterials are considered state-of-the-art sensing materials. This mini-review documents the advancement of nanomaterial-based CO2 sensors in the last two decades and discusses their strengths, weaknesses, and major applications. The use of nanomaterials for CO2 sensing offers several improvements in terms of selectivity, sensitivity, response time, and detection, demonstrating the advantage of using nanomaterials for developing high-performance CO2 sensors. Anticipated future trends in the area of nanomaterial-based CO2 sensors are also discussed in light of the existing limitations.
Improved Hardness and Thermal Stability of Nanocrystalline Nickel Electrodeposited with the Addition of Cysteine
Authors: Kolonits Tamás , Czigány Zsolt , Péter László , Bakonyi Imre , Gubicza Jenő
Published: November 2020

Experiments were conducted for the study of the effect of cysteine addition on the microstructure of nanocrystalline Ni films electrodeposited from a nickel sulfate-based bath. Furthermore, the thermal stability of the nanostructure of Ni layers processed with cysteine addition was also investigated. It was found that with increasing cysteine content in the bath, the grain size decreased, while the dislocation density and the twin fault probability increased. Simultaneously, the hardness increased due to cysteine addition through various effects. Saturation in the microstructure and hardness was achieved at cysteine contents of 0.3–0.4 g/L. Moreover, the texture changed from (220) to (200) with increasing the concentration of cysteine. The hardness of the Ni films processed with the addition of 0.4 g/L cysteine (∼6800 MPa) was higher than the values obtained for other additives in the literature (<6000 MPa). This hardness was further enhanced to ∼8400 MPa when the Ni film was heated up to 500 K. It was revealed that the hardness remained as high as 6000 MPa even after heating up to 750 K, while for other additives, the hardness decreased below 3000 MPa at the same temperature.
LINC00473 as an Immediate Early Gene under the Control of the EGR1 Transcription Factor
Authors: Aliperti Vincenza , Vitale Emilia , Aniello Francesco , Donizetti Aldo
Published: November 2020

Immediate early genes play an essential role in cellular responses to different stimuli. Many of them are transcription factors that regulate the secondary response gene expression. Non-coding RNAs may also be involved in this regulatory cascade. In fact, they are emerging as key actors of gene expression regulation, and evidence suggests that their dysregulation may underly pathological states. We previously took a snapshot of both coding and long non-coding RNAs differentially expressed in neuronal cells after brain-derived neurotrophic factor stimulation. Among these, the transcription factor EGR1 (a well-known immediate early gene) and LINC00473 (a primate-specific long non-coding RNA) that has emerged as an interesting RNA candidate involved in neuronal function and in cancer. In this work, we demonstrated that LINC00473 gene expression kinetics resembled that of immediate early genes in SH-SY5Y and HEK293T cells under different cell stimulation conditions. Moreover, we showed that the expression of LINC00473 is under the control of the transcription factor EGR1, providing evidence for an interesting functional relationship in neuron function.
Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia
Authors: Ogawa Kenjirou , Ishii Ayumi , Shindo Aimi , Hongo Kunihiro , Mizobata Tomohiro , Sogon Tetsuya , Kawata Yasushi
Published: November 2020

Neurological dementias such as Alzheimer’s disease and Lewy body dementia are thought to be caused in part by the formation and deposition of characteristic insoluble fibrils of polypeptides such as amyloid beta (Aβ), Tau, and/or α-synuclein (αSyn). In this context, it is critical to suppress and remove such aggregates in order to prevent and/or delay the progression of dementia in these ailments. In this report, we investigated the effects of spearmint extract (SME) and rosmarinic acid (RA; the major component of SME) on the amyloid fibril formation reactions of αSyn, Aβ, and Tau proteins in vitro. SME or RA was added to soluble samples of each protein and the formation of fibrils was monitored by thioflavin T (ThioT) binding assays and transmission electron microscopy (TEM). We also evaluated whether preformed amyloid fibrils could be dissolved by the addition of RA. Our results reveal for the first time that SME and RA both suppress amyloid fibril formation, and that RA could disassemble preformed fibrils of αSyn, Aβ, and Tau into non-toxic species. Our results suggest that SME and RA may potentially suppress amyloid fibrils implicated in the progression of Alzheimer’s disease and Lewy body dementia in vivo, as well.
Genetic Diversity of Cryptosporidium in Bactrian Camels (Camelus bactrianus) in Xinjiang, Northwestern China
Authors: Cao Yangwenna , Cui Zhaohui , Zhou Qiang , Jing Bo , Xu Chunyan , Wang Tian , Qi Meng , Zhang Longxian
Published: November 2020

Cryptosporidium species are ubiquitous enteric protozoan pathogens of vertebrates distributed worldwide. The purpose of this study was to gain insight into the zoonotic potential and genetic diversity of Cryptosporidium spp. in Bactrian camels in Xinjiang, northwestern China. A total of 476 fecal samples were collected from 16 collection sites in Xinjiang and screened for Cryptosporidium by PCR. The prevalence of Cryptosporidium was 7.6% (36/476). Six Cryptosporidium species, C. andersoni (n = 24), C. parvum (n = 6), C. occultus (n = 2), C. ubiquitum (n = 2), C. hominis (n = 1), and C. bovis (n = 1), were identified based on sequence analysis of the small subunit (SSU) rRNA gene. Sequence analysis of the gp60 gene identified six C. parvum isolates as subtypes, such as If-like-A15G2 (n = 5) and IIdA15G1 (n = 1), two C. ubiquitum isolates, such as subtype XIIa (n = 2), and one C. hominis isolate, such as Ixias IkA19G1 (n = 1). This is the first report of C. parvum, C. hominis, C. ubiquitum, and C. occultus in Bactrian camels in China. These results indicated that the Bactrian camel may be an important reservoir for zoonotic Cryptosporidium spp. and these infections may be a public health threat in this region.
Bioanalytical Assay Development and Validation for the Pharmacokinetic Study of GMC1, a Novel FKBP52 Co-chaperone Inhibitor for Castration Resistant Prostate Cancer
Authors: Ekpenyong Oscar , Cooper Candace , Ma Jing , Guy Naihsuan C. , Payan Ashley N. , Ban Fuqiang , Cherkasov Artem , Cox Marc B. , Liang Dong , Xie Huan
Published: November 2020

Background: GMC1 (2-(1H-benzimidazol-2-ylsulfanyl)-N-[(Z)-(4-methoxyphenyl) methylideneamino] acetamide) effectively inhibits androgen receptor function by binding directly to FKBP52. This is a novel mechanism for the treatment of castration resistant prostate cancer (CRPC). Methods: an LC-MS/MS method was developed and validated to quantify GMC1 in plasma and urine from pharmacokinetics studies in rats. An ultra-high-performance liquid chromatography (UHPLC) system equipped with a Waters XTerra MS C18 column was used for chromatographic separation by gradient elution with 0.1% (v/v) formic acid in water and methanol. A Sciex 4000 QTRAP® mass spectrometer was used for analysis by multiple reaction monitoring (MRM) in positive mode; the specific ions [M+H]+m/z 340.995 → m/z 191.000 and [M+H]+ m/z 266.013 → m/z 234.000 were monitored for GMC1 and internal standard (albendazole), respectively. Results: GMC1 and albendazole had retention times of 1.68 and 1.66 min, respectively. The calibration curves for the determination of GMC1 in rat plasma and urine were linear from 1–1000 ng/mL. The LC-MS/MS method was validated with intra- and inter-day accuracy and precision within the 15% acceptance limit. The extraction recovery values of GMC1 from rat plasma and urine were greater than 95.0 ± 2.1% and 97.6 ± 4.6%, respectively, with no significant interfering matrix effect. GMC1 is stable under expected sample handling, storage, preparation and LC-MS/MS analysis conditions. Conclusions: Pharmacokinetic evaluation of GMC1 revealed that the molecule has a biexponential disposition in rats, is distributed rapidly and extensively, has a long elimination half-life, and appears to be eliminated primarily by first order kinetics.
Evaluation of Actinium-225 Labeled Minigastrin Analogue [225Ac]Ac-DOTA-PP-F11N for Targeted Alpha Particle Therapy
Authors: Qin Yun , Imobersteg Stefan , Blanc Alain , Frank Stephan , Schibli Roger , Béhé Martin P. , Grzmil Michal
Published: November 2020

The overexpression of cholecystokinin B receptor (CCKBR) in human cancers led to the development of radiolabeled minigastrin analogues for targeted radionuclide therapy, which aims to deliver cytotoxic radiation specifically to cancer cells. Alpha emitters (e.g., actinium-225) possess high potency in cancer cell-killing and hold promise for the treatment of malignant tumors. In these preclinical studies, we developed and evaluated CCKBR-targeted alpha particle therapy. The cellular uptake and cytotoxic effect of actinium-225 labeled and HPLC-purified minigastrin analogue [225Ac]Ac-PP-F11N were characterized in the human squamous cancer A431 cells transfected with CCKBR. Nude mice bearing A431/CCKBR tumors were used for biodistribution and therapy studies followed by histological analysis and SPECT/CT imaging. In vitro, [225Ac]Ac-PP-F11N showed CCKBR-specific and efficient internalization rate and potent cytotoxicity. The biodistribution studies of [225Ac]Ac-PP-F11N revealed CCKBR-specific uptake in tumors, whereas the therapeutic studies demonstrated dose-dependent inhibition of tumor growth and extended mean survival time, without apparent toxicity. The histological analysis of kidney and stomach indicated no severe adverse effects after [225Ac]Ac-PP-F11N administration. The post-therapy SPECT-CT images with [111In]In-PP-F11N confirmed no CCKBR-positive tumor left in the mice with complete remission. In conclusion, our study demonstrates therapeutic efficacy of [225Ac]Ac-PP-F11N without acute radiotoxicity in CCKBR-positive cancer model.
Stability of Engineered Micro or Nanobubbles for Biomedical Applications
Authors: Park Beomjin , Yoon Semi , Choi Yonghyun , Jang Jaehee , Park Soomin , Choi Jonghoon
Published: November 2020

A micro/nanobubble (MNB) refers to a bubble structure sized in a micrometer or nanometer scale, in which the core is separated from the external environment and is normally made of gas. Recently, it has been confirmed that MNBs can be widely used in angiography, drug delivery, and treatment. Thus, MNBs are attracting attention as they are capable of constructing a new contrast agent or drug delivery system. Additionally, in order to effectively use an MNB, the method of securing its stability is also being studied. This review highlights the factors affecting the stability of an MNB and the stability of the MNB within the ultrasonic field. It also discusses the relationship between the stability of the bubble and its applicability in vivo.
Effect of Aqueous Extracts from Solidago Canadensis L. Leaves on Germination and Early Growth Stages of Three Cultivars of Raphanus Sativus L. Var. Radicula Pers
Authors: Możdżeń Katarzyna , Barabasz-Krasny Beata , Zandi Peiman , Kliszcz Angelika , Puła Joanna
Published: November 2020

Solidago canadensis L. is an expansive perennial that forms persistent, species-poor plant communities. It often spreads in fallow areas, displacing native floristic ingredients. Its expansiveness is largely due to morphological features, but it can also be the effect of allelopathic interaction. The aim of the experiment was to investigate the effect of aqueous extracts (decoction, infusion, and maceration) from dry S. canadensis leaves on germination and early growth stages of Raphanus sativus L. var. radicula Pers., in three cultivars: ‘Rowa’, ‘Półdługa’, and ‘Krakowianka’. In comparison to the control, the percentage of germinated radish seeds of ‘Rowa’ cultivar was statistically lower on the infusion and macerate. Regardless of the cultivar, the smallest changes in germination were found in seeds watered with decoction, and the largest with macerate. Seedlings length was most inhibited on macerate substrates, and least with infusion. Regardless of the form of the extract, each of them negatively affected the initial growth of radish seedlings. A fresh mass of ‘Rowa’ seedlings was inhibited by all Canadian goldenrod extracts. In relation to the control, the ‘Krakowianka’ cultivar was the least sensitive to S. canadensis extracts. The total chlorophyll content was the lowest in the seedlings of the ‘Rowa’ and ‘Półdługa’ cultivars germinated on macerate, compared to the control and two others extracts. The percentage of electrolyte leakage depended on the type of extract used and the radish cultivar. The study showed that depending on the cultivar, the aqueous extracts from S. canadensis leaves decreasing of germination and early growth of R. sativus.