bluuebunny/crossref_metadata_2025 huggingface dataset:
Reducing Generalization of Conditioned Fear: Beneficial Impact of Fear Relevance and Feedback in Discrimination Training
Authors: Herzog Katharina , Andreatta Marta , Schneider Kristina , Schiele Miriam A. , Domschke Katharina , Romanos Marcel , Deckert Jürgen , Pauli Paul
Published: June 2021

Anxiety patients over-generalize fear, possibly because of an incapacity to discriminate threat and safety signals. Discrimination trainings are promising approaches for reducing such fear over-generalization. Here we investigated the efficacy of a fear-relevant vs. a fear-irrelevant discrimination training on fear generalization and whether the effects are increased with feedback during training. Eighty participants underwent two fear acquisition blocks, during which one face (conditioned stimulus, CS+), but not another face (CS−), was associated with a female scream (unconditioned stimulus, US). During two generalization blocks, both CSs plus four morphs (generalization stimuli, GS1–GS4) were presented. Between these generalization blocks, half of the participants underwent a fear-relevant discrimination training (discrimination between CS+ and the other faces) with or without feedback and the other half a fear-irrelevant discrimination training (discrimination between the width of lines) with or without feedback. US expectancy, arousal, valence ratings, and skin conductance responses (SCR) indicated successful fear acquisition. Importantly, fear-relevant vs. fear-irrelevant discrimination trainings and feedback vs. no feedback reduced generalization as reflected in US expectancy ratings independently from one another. No effects of training condition were found for arousal and valence ratings or SCR. In summary, this is a first indication that fear-relevant discrimination training and feedback can improve the discrimination between threat and safety signals in healthy individuals, at least for learning-related evaluations, but not evaluations of valence or (physiological) arousal.
Attitudes of Local Population Towards the Impacts of Tourism Development: Evidence From Czechia
Authors: Linderová Ivica , Scholz Petr , Almeida Nuno
Published: June 2021

Increasing the socio-economic effects caused by the tourism development in the local population, they adopt some attitudes according to the impacts directly or indirectly perceived. However, some of this impact can be considered positive or negative, according to different perspectives. The issue of the resident-tourist relationship has been much-discussed recently. Therefore, many case studies are being conducted that address the impacts on both residents and tourists. The goal of this manuscript is to analyze the attitudes of local residents to the development of tourism in the urban monument zone Předhradí. Primary data were collected in a questionnaire survey for residents who have a permanent residence in a municipality of Předhradí in 2020. In our research, we tried to identify the significant negative impacts of tourism development. In the same way, we evaluate how the locals see positive effects on their quality of life conducted with tourism development. The research finds out that local respondents perceived some negative impacts to increase the economic perspective, as they referred to in the higher traffic load or increased noise. The pandemic crises are perceived as a game-changer in the tourism industry. For that reason, we suggest the primary considerations for future research not only with the academic perfective as for the practical point of view. The local population’s entrepreneurship attitudes must be one of the tools to assume the resilience toward the tourism development impacts.
The Relationship of Competitive Cognitive Anxiety and Motor Performance: Testing the Moderating Effects of Goal Orientations and Self-Efficacy Among Chinese Collegiate Basketball Players
Authors: Peng Fan , Zhang Li-Wei
Published: June 2021

The purpose of this study was to examine the moderating effects of goal orientations and self-efficacy between competitive cognitive anxiety and motor performance under conditions featuring different levels of ego-threat. Eighty-one (40 females) collegiate-level basketball players (M age = 20.26 years and SD = 2.68) completed Sport Competitive Anxiety Test, Ego Orientation in Sport Questionnaire, and General Self-Efficacy Scale prior to the experiment. Athletes participated in two sessions of free-throw tasks. After the first session, which was under a control condition, participants performed in a free-throw competitive session while being provided opponents’ scores that induced different levels of competitive cognitive anxiety. Performance is defined as the accuracy (%) in two free-throw sessions. A hierarchical multiple regression showed that high level of task-orientation and low level of ego-orientation can buffer the impairment of competitive cognitive anxiety on motor performance. The relationship between competitive cognitive anxiety and motor performance did not vary with self-efficacy. An a repeated-measured analysis of covariance after cluster analysis revealed that a high-task/low-ego profile benefited athletes the most regarding the impairment of competitive cognitive anxiety. Together, ego- and task-orientations and “goal profile” moderate the relationship between competitive cognitive anxiety and motor performance; however, self-efficacy may not serve as a moderator variable in between.
Disruption of Neural Activity and Functional Connectivity in Adolescents With Major Depressive Disorder Who Engage in Non-suicidal Self-Injury: A Resting-State fMRI Study
Authors: Huang Qian , Xiao Muni , Ai Ming , Chen Jianmei , Wang Wo , Hu Lan , Cao Jun , Wang Mengyao , Kuang Li
Published: June 2021

Background: Non-suicidal self-injury (NSSI), which commonly occurs during adolescence, often co-occurs with major depressive disorder (MDD). However, the underlying neurobiological mechanisms in adolescents with MDD who engage in NSSI remain unclear. The current study examined the aberrant local neural activity in certain areas of the visual regions and the default mode network (DMN) and the resting-state functional connectivity (rs-FC) in changed brain regions in adolescents with MDD who engage in NSSI and adolescents with MDD only.

Methods: A total of 67 adolescents with MDD were divided into two groups based on their NSSI behavior: the NSSI group (n = 31) and an age-, gender-, and education-matched MDD group (n = 36). The Hamilton Depression Rating Scale (HAMD) was used to assess the severity of MDD. Amplitude of low-frequency fluctuation (ALFF) analysis was used to detect alterations in local neural activity. Brain regions with aberrant neural activity were considered regions of interest (ROI). ALFF-based rs-FC analysis was used to further explore the underlying changes in connectivity between ROI and other areas in the NSSI group. Correlation analyses were performed to examine the relationship between neural changes and clinical characteristics.

Results: There was no significant difference in HAMD scores between the two groups. ALFF analysis revealed that, compared to adolescents with MDD only, adolescents with MDD who engaged in NSSI displayed significantly enhanced neural activity in the right fusiform gyrus (FFG. R) and the right median cingulate and paracingulate gyri (DCG. R). Significantly reduced rs-FC of the FFG. R-bilateral medial orbital of the superior frontal gyrus (ORBsupmed. L/R)/bilateral medial superior frontal gyrus (SFGmed. L/R), FFG. R-bilateral posterior cingulate gyrus (PCG. L/R), DCG. R-left pallidum (PAL. L), DCG. R-right superior temporal gyrus (STG. R), and DCG. R-right postcentral gyrus (PoCG. R)/right inferior parietal lobule (IPL. R) was found in adolescents with MDD who were engaged in NSSI. Additionally, no significant correlations were observed between ALFF or rs-FC values and the HAMD scores between the two groups.

Limitations: Owing to the cross-sectional design, the alterations in ALFF and rs-FC values in the FFG. R and DCG. R could not demonstrate that it was a state or feature in adolescents with MDD who engaged in NSSI. Additionally, the sample size was relatively small.

Conclusions: This study highlights changes in regional brain activity and remote connectivity in the FFG. R and DCG. R in adolescents with MDD who engage in NSSI. This could provide a new perspective for further studies on the neurobiological mechanism of NSSI behavior in adolescents with MDD.
Stress and Problematic Smartphone Use Severity: Smartphone Use Frequency and Fear of Missing Out as Mediators
Authors: Yang Haibo , Liu Bingjie , Fang Jianwen
Published: June 2021

Problematic smartphone use (PSU) has been linked with stress. Higher levels of stress likely increased problematic smartphone use. We investigated relations between stress, fear of missing out, and problematic smartphone use. The aim of the current study was to analyze the mediating role of fear of missing out (FOMO) and smartphone use frequency (SUF) between stress and PSU. We surveyed a broad sample of 2,276 Chinese undergraduate students in July 2019, using the FOMO Scale, Smartphone Addiction Scale-Short Version, Smartphone Use Frequency Scale, and Depression Anxiety Stress Scale-21. The results showed that stress was associated with PSU severity. Gender differences were found in PSU severity. Furthermore, FOMO was positively associated with SUF and PSU severity. Structural equation modeling demonstrated that FOMO acted as a mediator between stress and PSU severity. FOMO and SUF acted as a chain of mediators between stress and PSU severity. SUF did not account for relations between stress and PSU severity. The study indicates that FOMO may be an important variable accounting for why some people with increased stress levels may overuse their smartphones.
Multiperspective and Multimethod Evaluation of Flexible and Integrative Psychiatric Care Models in Germany: Study Protocol of a Prospective, Controlled Multicenter Observational Study (PsychCare)
Authors: Soltmann Bettina , Neumann Anne , March Stefanie , Weinhold Ines , Häckl Dennis , Kliemt Roman , Baum Fabian , Romanos Marcel , Schwarz Julian , von Peter Sebastian , Ignatyev Yuriy , Arnold Katrin , Swart Enno , Heinze Martin , Schmitt Jochen , Pfennig Andrea
Published: June 2021

Background: New cross-sectoral mental health care models have been initiated in Germany to overcome the fragmentation of the German health care system. Starting in 2013, flexible and integrative psychiatric care model projects according to §64b SGB V German Social Law (FIT64b) have been implemented. The study “PsychCare” combines quantitative and qualitative primary data with routine health insurance data for the evaluation of these models. Effects, costs and cost-effectiveness from the perspectives of patients, relatives and care providers are compared with standard care. Additionally, quality indicators for a modern, flexible and integrated care are developed. This article describes the rationale, design and methods of the project.

Methods: “PsychCare” is built on a multiperspective and multimethod design. A controlled prospective multicenter cohort study is conducted with three data collection points (baseline assessment, follow-up after 9 and 15 months). A total of 18 hospitals (10 FIT64b model and 8 matched control hospitals) have consecutively recruited in- and outpatients with pre-specified common and/or severe psychiatric disorders. Primary endpoints are differences in change of health-related quality of life and treatment satisfaction. Sociodemographic and service receipt data of the primary data collection are linked with routine health insurance data. A cost-effectiveness analysis, a mixed method, participatory process evaluation by means of qualitative surveys and the development of quality indicators are further elements of “PsychCare.”

Discussion and Practical Implications: The results based on data from different methodological approaches will provide essential conclusions for the improvement of hospital based mental health care in Germany. This should result in the identification of key FIT64b elements that can be efficiently implemented into standard care in Germany and re-structure the care strongly aligned to patient needs.

Clinical Trial Registration: German Clinical Trial Register, identifier DRKS 00022535.
Reinforcement Learning With Human Advice: A Survey
Authors: Najar Anis , Chetouani Mohamed
Published: June 2021

In this paper, we provide an overview of the existing methods for integrating human advice into a reinforcement learning process. We first propose a taxonomy of the different forms of advice that can be provided to a learning agent. We then describe the methods that can be used for interpreting advice when its meaning is not determined beforehand. Finally, we review different approaches for integrating advice into the learning process.
Case Report: Superior Vena Cava Resection and Reconstruction for Invasive Thyroid Cancer: Report of Three Cases and Literature Review
Authors: Chen Wenjie , Lei Jianyong , Wang Yichao , Tang Xiaojun , Liu Bin , Li Zhihui , Zhou Qinghua
Published: June 2021

Background: Thyroid cancer with massive invasion into the cervical and mediastinal great veins is extremely rare, and the surgical treatment is controversial, thus posing a great challenge for head and neck surgeons. Here, we report our successful experiences in reconstructing the superior vena cava (SVC) system to treat thyroid cancer with an extensive tumor thrombus growing intraluminally into the SVC.

Case Presentation: From September 2019 to September 2020, three patients with superior vena cava syndrome(SVCS) caused by tumor thrombus invasion from thyroid cancer were continuously included in this series. After preoperative evaluation, radical resection and reconstruction of the SVC system with expanded polytetrafluoroethylene (EPTFE) grafts were performed. In addition, bypass support from the right internal jugular vein to the right femoral vein was routinely prepared intraoperatively to prevent a rise in central venous pressure (CVP). Postoperatively, SVC-related syndrome improved immediately after the operation. Imaging examination showed good function of the reconstructed venous system. The patients recovered well with no surgical complications and remain under continuous follow-up.

Conclusions: Tumor growth into the SVC does not seem to be an absolute contraindication for surgery for thyroid carcinoma. Comprehensive treatment, including reconstruction of the SVC, is effective for relieving symptoms and preventing disease progression and is thus worth advocating. In addition, bypass support from the internal jugular vein to the femoral vein is easy to implement and can improve the safety of the operation.
Metabolomic and Lipidomic Approaches to Evaluate the Effects of Eucommia ulmoides Leaves on Milk Quality and Biochemical Properties
Authors: Teng Zhanwei , Wang Linfeng , Du Hongyan , Yang Gaiqing , Fu Tong , Lian Hongxia , Sun Yu , Liu Shenhe , Zhang Liyang , Gao Tengyun
Published: June 2021

Eucommia ulmoides leaves (EUL) contain a variety of natural bioactive compounds including chlorogenic acid, geniposide acid, and aucubin. These bioactive chemicals improve immune function and regulate lipid metabolism. The aim of this study was to investigate the effects of EUL on the biochemical properties of milk. Twenty Holstein dairy cows were randomly allocated to two groups fed a control (CTR, diet without EUL, n = 10) or EUL (diet containing 3% EUL, dry matter, n = 10) diet for 55 d. At the end of the experimental period (d 55), milk samples were collected and analyzed to determine their composition. Though levels of milk fat, protein, lactose, and total milk solids were similar between the groups, small molecules, metabolites, lipids, and cytokines differed. Compared with the CTR group, the EUL group had an improved cluster of differentiation (CD)4/CD8 ratio (P < 0.05) and lower interleukin (IL)-8 and IL-6 content (P < 0.05). Metabolomics analysis identified 14 metabolites including 7Z, 10Z, 13Z, 16Z, 19Z-docosapentaenoic acid (FC = 3.129), adrenic acid (FC = 2.830), and eicosapentaenoic acid (FC=1.685) as having significantly increased in the EUL group (P < 0.05) while 11 metabolites, including indole-2-carboxylic acid (FC = 0.636), cholic acid (FC = 0.430), and creatine (FC = 0.784) had significantly decreased (P < 0.05). Based on a constructed metabolome map, linoleic acid metabolism had the highest impact value for EUL. A total of 21 lipid classes and 1,094 lipid species were detected in the milk by lipidomic analysis, among which 40 differed significantly between the CTR and EUL groups. The present findings showed that the EUL altered milk composition. Correlation analysis showed that 7Z, 10Z, 13Z, 16Z, 19Z-docosapentaenoic acid, adrenic acid, and eicosapentaenoic acid levels were negatively correlated with those of the inflammatory factors IL-6 and IL-8 (P < 0.05), indicating that EUL improved milk quality by reducing inflammatory factors and increasing the CD4/CD8 ratio. Overall, our data demonstrate that EUL had positive effects on milk antioxidant parameters, immune indices, and micro-composition metabolism, thereby improving milk quality.
Case Report: Toxigenic Corynebacterium ulcerans Diphtheria-Like Infection in a Horse in the United Kingdom
Authors: Zendri Flavia , Isgren Cajsa Marie , Sinovich Matthew , Richards-Rios Peter , Hopkins Katie L. , Russell Katherine , Groves Natalie , Litt David , Fry Norman K. , Timofte Dorina
Published: June 2021

Corynebacterium ulcerans(C. ulcerans) may cause diphtheria in humans and can be carried by a wide range of animal species including dairy cows and, more recently, dogs and cats that have been increasingly involved in zoonotic trasmission. We isolated and characterized, by WGS, a toxigenicC. ulceransstrain from a diseased horse in the United Kingdom showing clinical signs of respiratory diphtheria comparable to those seen in people. Our results indicate a role for horses as reservoirs for zoonoticC. ulcerans.
Adaptive Versioning in Transactional Memory Systems
Authors: Poudel Pavan , Sharma Gokarna
Published: June 2021

Transactional memory has been receiving much attention from both academia and industry. In transactional memory, program code is split into transactions, blocks of code that appear to execute atomically. Transactions are executed speculatively and the speculative execution is supported through data versioning mechanism. Lazy versioning makes aborts fast but penalizes commits, whereas eager versioning makes commits fast but penalizes aborts. However, whether to use eager or lazy versioning to execute those transactions is still a hotly debated topic. Lazy versioning seems appropriate for write-dominated workloads and transactions in high contention scenarios whereas eager versioning seems appropriate for read-dominated workloads and transactions in low contention scenarios. This necessitates a priori knowledge on the workload and contention scenario to select an appropriate versioning method to achieve better performance. In this article, we present an adaptive versioning approach, called Adaptive, that dynamically switches between eager and lazy versioning at runtime, without the need of a priori knowledge on the workload and contention scenario but based on appropriate system parameters, so that the performance of a transactional memory system is always better than that is obtained using either eager or lazy versioning individually. We provide Adaptive for both persistent and non-persistent transactional memory systems using performance parameters appropriate for those systems. We implemented our adaptive versioning approach in the latest software transactional memory distribution TinySTM and extensively evaluated it through 5 micro-benchmarks and 8 complex benchmarks from STAMP and STAMPEDE suites. The results show significant benefits of our approach. Specifically, in persistent TM systems, our approach achieved performance improvements as much as 1.5× for execution time and as much as 240× for number of aborts, whereas our approach achieved performance improvements as much as 6.3× for execution time and as much as 170× for number of aborts in non-persistent transactional memory systems.
Approximation Algorithms for Sorting λ-Permutations by λ-Operations
Authors: Miranda Guilherme Henrique Santos , Alexandrino Alexsandro Oliveira , Lintzmayer Carla Negri , Dias Zanoni
Published: June 2021

Understanding how different two organisms are is one question addressed by the comparative genomics field. A well-accepted way to estimate the evolutionary distance between genomes of two organisms is finding the rearrangement distance, which is the smallest number of rearrangements needed to transform one genome into another. By representing genomes as permutations, one of them can be represented as the identity permutation, and, so, we reduce the problem of transforming one permutation into another to the problem of sorting a permutation using the minimum number of rearrangements. This work investigates the problems of sorting permutations using reversals and/or transpositions, with some additional restrictions of biological relevance. Given a value λ, the problem now is how to sort a λ-permutation, which is a permutation whose elements are less than λ positions away from their correct places (regarding the identity), by applying the minimum number of rearrangements. Each λ-rearrangement must have size, at most, λ, and, when applied to a λ-permutation, the result should also be a λ-permutation. We present algorithms with approximation factors of O(λ2), O(λ), and O(1) for the problems of Sorting λ-Permutations by λ-Reversals, by λ-Transpositions, and by both operations.
Research on Task Satellite Selection Method for Space Object Detection LEO Constellation Based on Observation Window Projection Analysis
Authors: Zhang Shengyu , Zhu Zhencai , Hu Haiying , Li Yuqing
Published: June 2021

Aiming at the task planning and scheduling problem of space object detection LEO constellation (SODLC) for detecting space objects in deep space background, a method of SODLC task satellite selection based on observation window projection analysis is proposed. This method projects the spatial relative relationships of the SODLC observation blind zone, observation range, and the initial spatial position of the objects onto the surface of the earth for detectable analysis of satellites and targets and binds the dynamic observation conditions to the satellite trajectory after projection calculation of the visible relationship between target changes. On this basis, combined with the features of SODLC with high orbital symmetry, the task satellite selection is divided into two steps: orbit plane selection and task satellite selection. The orbit planes are selected based on the longitude range of the ascending node with the geographic location of the targets, and the task satellites are selected according to the relative motion relationship between the satellites and the targets together with the constraints of observable conditions. The selection method simplifies the calculation process of scheduling and selecting task satellites. Simulation analysis prove the method has better task satellite selection efficiency. The method has high practical value for task planning and scheduling for event-driven SODLC.
Aroma Profile, Microbial and Chemical Quality of Ensiled Green Forages Mixtures of Winter Cereals and Italian Ryegrass
Authors: Worku Alemayehu , Tóth Tamás , Orosz Szilvia , Fébel Hedvig , Kacsala László , Húth Balázs , Hoffmann Richárd , Yakubu Haruna Gado , Bazar George , Tóthi Róbert
Published: June 2021

The objective of this study was to evaluate the aroma profile, microbial and chemical quality of winter cereals (triticale, oats, barley and wheat) and Italian ryegrass (Lolium multiflorum Lam., IRG) plus winter cereal mixture silages detected with an electronic nose. Four commercial mixtures (mixture A (40% of two cultivars of winter triticale + 30% of two cultivars of winter oats + 20% of winter barley + 10% of winter wheat), mixture B (50% of two cultivars of winter triticale + 40% of winter barley + 10% of winter wheat), mixture C (55% of three types of Italian ryegrass + 45% of two cultivars of winter oat), mixture D (40% of three types of Italian ryegrass + 30% of two cultivars of winter oat + 15% of two cultivars of winter triticale + 10% of winter barley + 5% of winter wheat)) were harvested, wilted and ensiled in laboratory-scale silos (n = 80) without additives. Both the principal component analysis (PCA) score plot for aroma profile and linear discriminant analysis (LDA) classification revealed that mixture D had different aroma profile than other mixture silages. The difference was caused by the presence of high ethanol and LA in mixture D. Ethyl esters such as ethyl 3-methyl pentanoate, 2-methylpropanal, ethyl acetate, isoamyl acetate and ethyl-3-methylthiopropanoate were found at different retention indices in mixture D silage. The low LA and higher mold and yeast count in mixture C silage caused off odour due to the presence of 3-methylbutanoic acid, a simple alcohol with unpleasant camphor-like odor. At the end of 90 days fermentation winter cereal mixture silages (mixture A and B) had similar aroma pattern, and mixture C was also similar to winter cereal silages. However, mixture D had different aromatic pattern than other ensiled mixtures. Mixture C had higher (p < 0.05) mold and yeast (Log10 CFU (colony forming unit)/g) counts compared to mixture B. Mixture B and C had higher acetic acid (AA) content than mixture A and D. The lactic acid (LA) content was higher for mixture B than mixture C. In general, the electronic nose (EN) results revealed that the Italian ryegrass and winter cereal mixtures (mixture D) had better aroma profile as compared to winter cereal mixtures (mixture A and B). However, the cereal mixtures (mixture A and B) had better aroma quality than mixture C silage. Otherwise, the EN technology is suitable in finding off odor compounds of ensiled forages.
Assessing the Genetic Diversity and Population Structure of a Tunisian Melon (Cucumis melo L.) Collection Using Phenotypic Traits and SSR Molecular Markers
Authors: Chikh-Rouhou Hela , Mezghani Najla , Mnasri Sameh , Mezghani Neila , Garcés-Claver Ana
Published: June 2021

The assessment of genetic diversity and structure of a gene pool is a prerequisite for efficient organization, conservation, and utilization for crop improvement. This study evaluated the genetic diversity and population structure of 24 Tunisian melon accessions, by using 24 phenotypic traits and eight microsatellite (SSR) markers. A considerable phenotypic diversity among accessions was observed for many characters including those related to agronomical performance. All the microsatellites were polymorphic and detected 30 distinct alleles with a moderate (0.43) polymorphic information content. Shannon’s diversity index (0.82) showed a high degree of polymorphism between melon genotypes. The observed heterozygosity (0.10) was less than the expected heterozygosity (0.12), displaying a deficit in heterozygosity because of selection pressure. Molecular clustering and structure analyses based on SSRs separated melon accessions into five groups and showed an intermixed genetic structure between landraces and breeding lines belonging to the different botanical groups. Phenotypic clustering separated the accessions into two main clusters belonging to sweet and non-sweet melon; however, a more precise clustering among inodorus, cantalupensis, and reticulatus subgroups was obtained using combined phenotypic–molecular data. The discordance between phenotypic and molecular data was confirmed by a negative correlation (r = −0.16, p = 0.06) as revealed by the Mantel test. Despite these differences, both markers provided important information about the diversity of the melon germplasm, allowing the correct use of these accessions in future breeding programs. Together they provide a powerful tool for future agricultural and conservation tasks.
Connecting the Lab and the Field: Genome Analysis of Phyllobacterium and Rhizobium Strains and Field Performance on Two Vegetable Crops
Authors: Flores-Félix José David , Velázquez Encarna , Martínez-Molina Eustoquio , González-Andrés Fernando , Squartini Andrea , Rivas Raúl
Published: June 2021

The legume nodules are a rich source not only of rhizobia but also of endophytic bacteria exhibiting plant growth-promoting mechanisms with potential as plant biostimulants. In this work we analyzed the genomes of Phyllobacterium endophyticum PEPV15 and Rhizobium laguerreae PEPV16 strains, both isolated from Phaseolus vulgaris nodules. In silico analysis showed that the genomes of these two strains contain genes related to N-acyl-homoserine lactone (AHL) and cellulose biosynthesis, involved in quorum sensing and biofilm formation, which are essential for plant colonization. Several genes involved in plant growth promotion such as those related to phosphate solubilization, indole acetic acid production, siderophore biosynthesis and nitrogen fixation were also located in both genomes. When strains PEPV15 and PEPV16 were inoculated in lettuce and carrot in field assays, we found that both significantly increased the yield of lettuce shoots and carrot roots by more than 20% and 10%, respectively. The results of this work confirmed that the genome mining of genes involved in plant colonization and growth promotion is a good strategy for predicting the potential of bacterial strains as crops inoculants, opening new horizons for the selection of bacterial strains with which to design new, effective bacteria-based plant biostimulants.
Evaluation of a New Generation of Coated Fertilizers to Reduce the Leaching of Mineral Nutrients and Greenhouse Gas (N2O) Emissions
Authors: Benlamlih Fatima Zahra , Lamhamedi Mohammed S. , Pepin Steeve , Benomar Lahcen , Messaddeq Younès
Published: June 2021

The increased use of fertilizers in agriculture and forest and horticulture nurseries contributes to the pollution of water resources and greenhouse gas emissions. The objective of this study is to evaluate a new generation of fertilizers coated with new biodegradable polymers in terms of physical quality, release kinetics, and their effect on reducing nitrate leaching and N2O emissions and compare them to uncoated fertilizers (Urea, monoammonium phosphate (MAP), and KCl) having the same mineral nutrient concentration. In a peat-based substrate, the release of mineral nutrients was similar in both types of fertilizer. Two hours after application, Urea released 34% more urea than Biodrix N, the difference disappearing after one day. The leaching of cumulative ammonium nitrogen after 20 days was reduced by 40% and 26% respectively by Aminaex and Biodrix N compared to Urea. In a peat-based substrate containing 30% (v/v) of compost, the cumulative nitrate leaching was reduced by 54% by Biodrix N and by 41% by Aminaex compared to Urea. The highest average N2O flux was observed on the first day for Urea, whereas for Aminaex and Biodrix N, N2O emissions increased on the third day, reaching a peak of efflux on day 10. A 10-day delay of the N2O efflux emissions and a longer period of emissions were observed in treatments containing Aminaex and Biodrix N compared to Urea. Cumulative N2O efflux was 142, 154, and 171 mg m−2, respectively, for Urea, Aminaex, and Biodrix N over a 20-day period. These new biodegradable polymer-coated nitrogen fertilizers can reduce mineral nutrient leaching in the event of heavy rainfall and lower maximum N2O emissions in comparison with conventional nitrogen sources.
Spontaneous Behaviors of Post-Orchiectomy Pain in Horses Regardless of the Effects of Time of Day, Anesthesia, and Analgesia
Authors: Trindade Pedro Henrique Esteves , Taffarel Marilda Onghero , Luna Stelio Pacca Loureiro
Published: June 2021

This prospective and longitudinal study aimed to identify spontaneous post-orchiectomy pain behaviors in horses regardless of the effects of anesthesia, analgesia, and recording time of day. Twenty-four horses divided into four groups were submitted to: inhalation anesthesia only (GA), or combined with previous analgesia (GAA), or orchiectomy under pre (GCA), or postoperative (GC) analgesia. The data obtained from the subtraction of frequency and/or duration of 34 behaviors recorded during seven 60-min time-points in the 24 h after the anesthesia from those recorded in the mirrored time-points in the 24 h before the anesthesia (delta) were compared over time and among groups by Friedman and Kruskal–Wallis tests, respectively (p < 0.05). Time of day influenced the behaviors of walk, look out the window, rest the pelvic limb, and rest standing still. The only pain-related behaviors were decreased mirrored proportional differences in time spent drinking, and eating, and increased mirrored proportional differences in the frequency or duration of look at the wound, retract the pelvic limb, expose the penis, and look at the back of the stall. In conclusion, confounding factors rather than pain may influence several suggestive pain-related behaviors documented in the literature.
Porcine Circovirus 3a Field Strains in Free-Living Wild Boars in Paraná State, Brazil
Authors: de Souza Tatiana Carolina Gomes Dutra , Gava Danielle , Schaefer Rejane , Leme Raquel Arruda , da Silva Porto Gisele , Alfieri Amauri Alcindo
Published: June 2021

Porcine circovirus 3 (PCV-3) was identified in domestic pigs worldwide. Although PCV-3 has also been detected in wild boars, information regarding its circulation in this free-living animal species is scarce. To investigate PCV-3 occurrence in free-living wild boars in Brazil, 70 serum samples collected between January 2017 and June 2019 in Paraná state, Brazil were analyzed by PCR assay. Amplicons measuring 330 bp in length were amplified in seven (10.0%) of the serum samples and confirmed to be PCV3-specific by nucleotide (nt) sequencing. As the amplified products from the serum samples yielded only intermediate levels of viral DNA, lung samples from the seven PCR-positive wild boars were also evaluated by PCR. Of these samples, five lung samples were positive and provided high levels of viral DNA. The three lung samples that presented the highest levels of viral DNA were selected for amplification and sequencing of the whole PCV-3 genome. The three full-length sequences obtained were grouped in PCV-3 clade “a”, and the sequences exhibited 100% nucleotide similarity among them. The PCV-3 field strains of this study showed nucleotide and amino acid similarities of 98.5–99.8% and 98.8–100%, respectively, with whole-genome PCV-3 sequences from around the world.
Energy Requirements of Beef Cattle: Current Energy Systems and Factors Influencing Energy Requirements for Maintenance
Authors: Cabezas-Garcia Edward H. , Lowe Denise , Lively Francis
Published: June 2021

The present review compared features of the UK system for predicting energy requirements in beef cattle with a number of feeding systems developed from research institutes consortiums around the world. In addition, energy requirements for maintenance calculated from studies conducted at the Agri-Food and Biosciences Institute (AFBI) in Northern Ireland since the 1990s were compared with compiled data from recent peer-review papers published over the last decade (2009–2020). The mean metabolisable energy requirement for the maintenance (MEm) of growing cattle was 0.672 MJ/kg0.75 according to values obtained from calorimetry studies conducted at AFBI. This value is respectively 8.2 and 19.5% greater than the MEm values obtained by the Agricultural and Food Research Council (AFRC), and the National Academies of Sciences, Engineering and Medicine (NASEM) equations, but it is in close agreement with the Institut National de la Recherche Agronomique (INRA) approach, when assuming a Bos taurus bull (300 kg LW) and an efficiency for converting energy for maintenance (km) of 0.65. Most of the literature data on energy requirements for the maintenance for this animal category were obtained from studies conducted with Bos indicus animals and their crossbreds in Brazilian conditions with this confirming lower requirements of these animals when compared to pure Bos taurus cattle. A simulation of the total ME requirements calculated for an Angus × Friesian steer (LW = 416 kg) offered good quality grass silage, indicated that both AFRC and NASEM systems overestimate (38.5 and 20.5%, respectively) the observed efficiency of converting ME for growth (kg). When the total ME requirements (maintenance + growth) were assessed, both systems underpredicted total ME requirement in 15.8 and 22.1 MJ/d. The mean MEm requirements for suckler cows obtained from the literature (0.596 MJ/kg0.75) is on average 19.1% greater than predictions given by both AFRC and INRA (lactation) equations when considering a 550 kg cow and a km value of 0.72. Although no differences in net energy requirements for maintenance (NEm) were detected between dry and lactating suckler cows, as expected the later displayed greater variation as a result of differences in milk production. On this regard, the INRA model recognise increased NEm requirements for lactating animals compared to dry cows. The re-evaluation of the concept of diet metabolisability and the analysis of existing data on compensatory growth responses are recommended for future updates of the British system (AFRC) having in to account the particularities of grass-based systems in the UK.
Towards Machine Recognition of Facial Expressions of Pain in Horses
Authors: Andersen Pia Haubro , Broomé Sofia , Rashid Maheen , Lundblad Johan , Ask Katrina , Li Zhenghong , Hernlund Elin , Rhodin Marie , Kjellström Hedvig
Published: June 2021

Automated recognition of human facial expressions of pain and emotions is to a certain degree a solved problem, using approaches based on computer vision and machine learning. However, the application of such methods to horses has proven difficult. Major barriers are the lack of sufficiently large, annotated databases for horses and difficulties in obtaining correct classifications of pain because horses are non-verbal. This review describes our work to overcome these barriers, using two different approaches. One involves the use of a manual, but relatively objective, classification system for facial activity (Facial Action Coding System), where data are analyzed for pain expressions after coding using machine learning principles. We have devised tools that can aid manual labeling by identifying the faces and facial keypoints of horses. This approach provides promising results in the automated recognition of facial action units from images. The second approach, recurrent neural network end-to-end learning, requires less extraction of features and representations from the video but instead depends on large volumes of video data with ground truth. Our preliminary results suggest clearly that dynamics are important for pain recognition and show that combinations of recurrent neural networks can classify experimental pain in a small number of horses better than human raters.
Leukocytes Ratios in Feline Systemic Inflammatory Response Syndrome and Sepsis: A Retrospective Analysis of 209 Cases
Authors: Gori Eleonora , Pierini Alessio , Lippi Ilaria , Lubas George , Marchetti Veronica
Published: June 2021

Sepsis is a challenging condition in which hematological prognostic and diagnostic markers in cats are limited. The aims of this study were to test if there are any differences in leukocyte ratios (NLR, BLR and BNLR) between healthy, SIRS and septic cats (sick cats), and if, within sick cats, NLR, BLR and BNLR may be prognostic markers. A retrospective medical database study included 76 healthy cats (blood-donors), 54 SIRS and 79 septic cats. SIRS group was defined if cats fulfilled SIRS criteria. Sepsis was confirmed with an infectious focus on cytology or a positive culture for bacterial infection. Leukocyte ratios were compared among the three study groups and between survivors and non-survivors in sick cats. NLR resulted significantly higher in the sick group compared to healthy cats (p < 0.0001), although NLR was not different between SIRS and sepsis. An NLR > 4.53 had a sensitivity of 76% and a specificity of 93.4% to detect SIRS/sepsis (OR 44.8 95%CI 17–107). Only BLR and BNLR were significantly different between SIRS and sepsis. NLR was associated with mortality in the sick group (p = 0.04). Although NLR resulted higher in sick cats than healthy, BLR and BNLR demonstrated as promising tools in differentiating SIRS from sepsis. NLR was associated with mortality in sick cats.
Lorenz Plot Analysis in Dogs with Sinus Rhythm and Tachyarrhythmias
Authors: Romito Giovanni , Guglielmini Carlo , Poser Helen , Baron Toaldo Marco
Published: June 2021

The Lorenz plot (LP), a graphical representation of heart rate variability, has been poorly studied in dogs to date. The present study aimed to describe the graphic features of LP in dogs with sinus rhythm (SR) and tachyarrhythmias, and to analyze the usefulness of its pattern recognition. One hundred and nineteen canine Holter recordings were retrospectively evaluated. Cardiac rhythms were classified as: SR; SR with frequent (>100) premature ectopies (atrial, SR-APCs; ventricular, SR-VPCs; atrial and ventricular, SR-APCs-VPCs); atrial fibrillation (AF); and AF with frequent VPCs (AF-VPCs). Lorenz plots were studied qualitatively and quantitatively, and classified by distinct LP patterns (LPPs). Repeatability and reproducibility of LPP classification and diagnostic value were determined. Recordings included: 48 SR, 9 SR-APCs, 35 SR-VPCs, 5 SR-APCs-VPCs, 4 AF, and 18 AF-VPCs. Ten LPPs were identified: comet (n = 12), torpedo (n = 3), Y-shaped (n = 6), diamond (n = 10), diamond with a central silent zone (n = 17), double side-lobe (DSL) (n = 47), triple side-lobe (n = 1), quadruple side-lobe (n = 2), fan (n = 18), and fan with DSL (n = 3). Repeatability and reproducibility of LPP classification were excellent. The DSL pattern was both highly sensitive (91.3%) and specific (94.5%) for SR with frequent premature ectopies, either APCs, or VPCs, or both. The remaining LPPs had lower diagnostic value (high specificity but low sensitivity). Distinct rhythms imprint distinct and reproducible LPPs in dogs. The majority of canine LPPs are specific but insensitive indicators of SR and tachyarrhythmias.
Colistin Resistance and ESBL Production in Salmonella and Escherichia coli from Pigs and Pork in the Thailand, Cambodia, Lao PDR, and Myanmar Border Area
Authors: Lay Khin Khin , Jeamsripong Saharuetai , Sunn Kyaw Phyoe , Angkititrakul Sunpetch , Prathan Ransiya , Srisanga Songsak , Chuanchuen Rungtip
Published: June 2021

The study aimed to examine the prevalence and genetic characteristics of ESBL-production and colistin resistance in Salmonella and Escherichia coli from pigs and pork in the border area among Thailand, Cambodia, Lao PDR, and Myanmar. Salmonella (n = 463) and E. coli (n = 767) isolates were collected from pig rectal swab from slaughterhouses (n = 441) and pork from retail markets (n = 368) during October 2017 and March 2018. All were determined for susceptibility to colistin and cephalosporins, ESBL production and mcr and ESBL genes. Salmonella was predominantly found in Cambodia (65.8%). Serovars Rissen (35.6%) and Anatum (15.3%) were the most common. The E. coli prevalence in pork was above 91% in all countries. Colistin-resistance rate in E. coli (10.4%) was significantly higher than Salmonella (2.6%). ESBL-producing Salmonella (1.9%) and E. coli (6.3%) were detected. The blaCTX-M-55 and blaCTX-M-14 were identified. The mcr-1 gene was detected in Salmonella (n = 12) and E. coli (n = 68). The mcr-1/blaCTX-M-55 and mcr-3/blaCTX-M-55 co-concurrence was observed in one Salmonella and three E. coli isolates, respectively. In conclusion, pigs and pork serve as carriers of colistin and new generation cephalosporins resistance. Testing for resistance to last line antibiotics should be included in national AMR surveillance program using One Health approach.
Danish GPs’ Experiences When Managing Patients Presenting to General Practice with Symptoms of Acute Lower Respiratory Tract Infections: A Qualitative Study
Authors: Bisgaard Lise , Andersen Camilla Aakjær , Jensen Morten Sig Ager , Bjerrum Lars , Hansen Malene Plejdrup
Published: June 2021

One of the most common indications for antibiotic prescribing in general practice is acute lower respiratory tract infections (LRTI). This study aimed to explore general practitioners’ (GPs’) considerations and experiences when managing patients with symptoms of an acute LRTI. Individual semi-structured interviews were conducted with seven GPs in the North Denmark Region from January to March 2020. Data were analysed by means of systematic text condensation. The analysis revealed four themes: (1) practicalities of assessing patients with LRTI, (2) assessment of the patient, (3) treatment decisions, and (4) patient expectations. The GPs described having developed individual diagnostic strategies and routines when managing patients with symptoms of an acute LRTI. However, a general assessment of the patient was essential to all the GPs and the diagnosis was seldom based on a single symptom or finding. Most GPs described having great faith in abnormal lung auscultation. The use of C-reactive protein testing served several purposes, such as deciding on the severity of the infection, prescribing antibiotics or not, and as a communicative tool. Diagnostic uncertainty is a driver of antibiotic use and clinical practice might benefit from the development of clinical prediction rules for diagnosing pneumonia.
Volatile Organic Compounds (VOCs) Produced by Gluconobacter cerinus and Hanseniaspora osmophila Displaying Control Effect against Table Grape-Rot Pathogens
Authors: Delgado Ninoska , Olivera Matías , Cádiz Fabiola , Bravo Guillermo , Montenegro Iván , Madrid Alejandro , Fuentealba Claudia , Pedreschi Romina , Salgado Eduardo , Besoain Ximena
Published: June 2021

Table grapes (Vitis vinifera) are affected by botrytis bunch rot and summer bunch rot, the latter a complex disease caused by Botrytis cinerea, Aspergillus spp., Penicillium expansum and Rhizopus stolonifer. To search for biocontrol alternatives, a new bioproduct composed of Gluconobacter cerinus and Hanseniaspora osmophila, a consortium called PUCV-VBL, was developed for the control of fungal rots in table grapes. Since this consortium presents new biocontrol species, the effect of their VOCs (volatile organic compounds) was evaluated under in vitro and in vivo conditions. The VOCs produced by the PUCV-VBL consortium showed the highest mycelial inhibition against Botrytis cinerea (86%). Furthermore, H. osmophila was able to inhibit sporulation of A. tubingensis and P. expansum. VOCs’ effect in vivo was evaluated using berries from Red Globe, Thompson Seedless and Crimson Seedless grapes cultivars, demonstrating a mycelial inhibition by VOCs greater than 70% for all evaluated fungal species. The VOC identification of the PUCV-VBL consortium was analyzed by solid-phase microextraction coupled to gas chromatography-mass spectrometry (SPME-GCMS). A total 26 compounds were identified, including 1-butanol 3-methyl, propanoic acid ethyl ester, ethyl acetate, phenylethyl alcohol, isobutyl acetate and hexanoic acid ethyl ester. Our results show that VOCs are an important mode of action of the PUCV-VBL biological consortium.