bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of Class-3 Semaphorin Receptors, Neuropilins and Plexins, as Therapeutic Targets in a Pan-Cancer Study
Authors: Zhang Xiaoli , Shao Shuai , Li Lang
Published: July 2020

Class-3 semaphorins (SEMA3s), initially characterized as axon guidance cues, have been recognized as key regulators for immune responses, angiogenesis, tumorigenesis and drug responses. The functions of SEMA3s are attributed to the activation of downstream signaling cascades mainly mediated by cell surface receptors neuropilins (NRPs) and plexins (PLXNs), yet their roles in human cancers are not completely understood. Here, we provided a detailed pan-cancer analysis of NRPs and PLXNs in their expression, and association with key signal transducers, patient survival, tumor microenvironment (TME), and drug responses. The expression of NRPs and PLXNs were dysregulated in many cancer types, and the majority of them were further dysregulated in metastatic tumors, indicating a role in metastatic progression. Importantly, the expression of these genes was frequently associated with key transducers, patient survival, TME, and drug responses; however, the direction of the association varied for the particular gene queried and the specific cancer type/subtype tested. Specifically, NRP1, NRP2, PLXNA1, PLXNA3, PLXNB3, PLXNC1, and PLXND1 were primarily associated with aggressive phenotypes, whereas the rest were more associated with favorable prognosis. These data highlighted the need to study each as a separate entity in a cancer type- and subtype-dependent manner.
Sex and Gender Disparities in Melanoma
Authors: Bellenghi Maria , Puglisi Rossella , Pontecorvi Giada , De Feo Alessandra , Carè Alessandra , Mattia Gianfranco
Published: July 2020

Worldwide, the total incidence of cutaneous melanoma is higher in men than in women, with some differences related to ethnicity and age and, above all, sex and gender. Differences exist in respect to the anatomic localization of melanoma, in that it is more frequent on the trunk in men and on the lower limbs in women. A debated issue is if—and to what extent—melanoma development can be attributed to gender-specific behaviors or to biologically intrinsic differences. In the search for factors responsible for the divergences, a pivotal role of sex hormones has been observed, although conflicting results indicate the involvement of other mechanisms. The presence on the X chromosome of numerous miRNAs and coding genes playing immunological roles represents another important factor, whose relevance can be even increased by the incomplete X chromosome random inactivation. Considering the known advantages of the female immune system, a different cancer immune surveillance efficacy was suggested to explain some sex disparities. Indeed, the complexity of this picture emerged when the recently developed immunotherapies unexpectedly showed better improvements in men than in women. Altogether, these data support the necessity of further studies, which consider enrolling a balanced number of men and women in clinical trials to better understand the differences and obtain actual gender-equitable healthcare.
XRN2 Links RNA:DNA Hybrid Resolution to Double Strand Break Repair Pathway Choice
Authors: Dang Tuyen T. , Morales Julio C.
Published: July 2020

It was recently shown that the 5’ to 3’ exoribonuclease XRN2 is involved in the DNA damage response. Importantly, loss of XRN2 abrogates DNA double stranded break repair via the non-homologous end-joining pathway. However, the mechanistic details of how XRN2 functions in the non-homologous end-joining repair process are unknown. In this study, we elucidated that XRN2-mediated RNA:DNA hybrid resolution is required to allow Ku70 binding to DNA ends. These data suggest that XRN2 is required for the initiation of non-homologous end-joining repair. Interestingly, we uncovered a role for XRN2 in the homologous recombination repair pathway. Loss of XRN2 lead to a decrease in the repair of double strand breaks by homologous recombination. Strikingly, when we removed RNA:DNA hybrids by RNaseH1 over-expression, homologous recombination was not restored. We found RNA:DNA hybrid formation at and downstream of the DSB site, suggesting that unregulated transcription inhibits homologous recombination repair. In summary, our results indicate a relation between RNA:DNA hybrid resolution and double strand break repair pathway choice.
High Active PdSn Binary Alloyed Catalysts Supported on B and N Codoped Graphene for Formic Acid Electro-Oxidation
Authors: Chen Dan , Pei Shien , He Zhishun , Shao Haibo , Wang Jianming , Wang Kai , Wang Yong , Jin Yanxian
Published: July 2020

A series of PdSn binary catalysts with varied molar ratios of Pd to Sn are synthesized on B and N dual-doped graphene supporting materials. The catalysts are characterized by X-ray diffraction (XRD) and Transmission electron microscopy (TEM). Formic acid electro-oxidation reaction is performed on these catalysts, and the results reveal that the optimal proportion of Pd:Sn is 3:1. X-ray photoelectron spectroscopy (XPS) measurements show that when compared with 3Pd1Sn/graphene, B and N co-doping into the graphene sheet can tune the electronic structure of graphene, favoring the formation of small-sized metallic nanoparticles with good dispersion. On the other hand, when compared with the monometallic counterparts, the incorporation of Sn can generate oxygenated species that help to remove the intermediates, exposing more active Pd sites. Moreover, the electrochemical tests illustrate that 3Pd1Sn/BN-G catalyst with a moderate amount of Sn exhibits the best catalytic activity and stability on formic acid electro-oxidation, owing to the synergistic effect of the Sn doping and the B, N co-doping graphene substrate.
Buildings Energy Efficiency Analysis and Classification Using Various Machine Learning Technique Classifiers
Authors: Benavente-Peces César , Ibadah Nisrine
Published: July 2020

Energy efficiency is a major concern to achieve sustainability in modern society. Smart cities sustainability depends on the availability of energy-efficient infrastructures and services. Buildings compose most of the city, and they are responsible for most of the energy consumption and emissions to the atmosphere (40%). Smart cities need smart buildings to achieve sustainability goals. Building’s thermal modeling is essential to face the energy efficiency race. In this paper, we show how ICT and data science technologies and techniques can be applied to evaluate the energy efficiency of buildings. In concrete, we apply machine learning techniques to classify buildings based on their energy efficiency. Particularly, our focus is on single-family buildings in residential areas. Along this paper, we demonstrate the capabilities of machine learning techniques to classify buildings depending on their energy efficiency. Moreover, we analyze and compare the performance of different classifiers. Furthermore, we introduce new parameters which have some impact on the buildings thermal modeling, especially those concerning the environment where the building is located. We also make an insight on ICT and remark the growing relevance in data acquisition and monitoring of relevant parameters by using wireless sensor networks. It is worthy to remark the need for an appropriate and reliable dataset to achieve the best results. Moreover, we demonstrate that reliable classification is feasible with a few featured parameters.
Absorption of Cu(II) and Zn(II) from Aqueous Solutions onto Biochars Derived from Apple Tree Branches
Authors: Zhao Shixiang , Ta Na , Wang Xudong
Published: July 2020

The aim of this study was to investigate the adsorption of Cu(II) and Zn(II) onto apple tree branches biochar (BC) produced at 300, 400, 500 and 600 °C (BC300, BC400, BC500, and BC600), respectively. The effect of adsorbent dosage, pH value, contact time, initial concentration of Cu(II) or Zn(II), and temperature on the adsorption process were investigated. The result showed that 5 g BC·L−1 was the optimal dosage to remove Cu(II) and Zn(II) from wastewater and the maximum adsorption efficiency was achieved at a pH of 5.0 for all the BCs when the initial concentration of Cu(II) and Zn(II) were 64 and 65 mg L−1, respectively. Adsorption kinetics and isotherm experiments showed that the pseudo-second order equation and the Langmuir isotherm could best describe the adsorption process, indicating that the adsorption of Cu(II) and Zn(II) onto BCs were monolayer processes and chemisorption was the rate limiting step. The values of ΔG0 for the absorption of Cu(II) and Zn(II) on all BCs were negative, while the values of ΔH0 were positive, suggesting that the absorption was a spontaneous endothermic process. The mechanisms of BC adsorption of metal ions adsorption include surface precipitation, ion exchange, and minor contribution by cation-π interaction. BC500 had highest Cu(II) and Zn(II) adsorption capacity under various conditions (except at pH 2.0). The maximum adsorption capacities of Cu(II) and Zn(II) on BC500 were 11.41 and 10.22 mg·g−1, respectively. Therefore, BC derived from apple tree branches produced at 500 °C can be used as an adsorbent to remove Cu(II) and Zn(II) from wastewater.
Design of Nanomaterials for Hydrogen Storage
Authors: Pasquini Luca
Published: July 2020

The interaction of hydrogen with solids and the mechanisms of hydride formation experience significant changes in nanomaterials due to a number of structural features. This review aims at illustrating the design principles that have recently inspired the development of new nanomaterials for hydrogen storage. After a general discussion about the influence of nanomaterials’ microstructure on their hydrogen sorption properties, several scientific cases and hot topics are illustrated surveying various classes of materials. These include bulk-like nanomaterials processed by mechanochemical routes, thin films and multilayers, nano-objects with composite architectures such as core–shell or composite nanoparticles, and nanoparticles on porous or graphene-like supports. Finally, selected examples of recent in situ studies of metal–hydride transformation mechanisms using microscopy and spectroscopy techniques are highlighted.
Frequency Stability Evaluation in Low Inertia Systems Utilizing Smart Hierarchical Controllers
Authors: Patsalides Minas , Papadimitriou Christina N. , Efthymiou Venizelos , Ciavarella Roberto , Di Somma Marialaura , Wakszyńska Anna , Kosmecki Michał , Graditi Giorgio , Valenti Maria
Published: July 2020

The high penetration of the Renewable Energy Sources and other emerging technologies likely to be installed in future power grids will pose new operational challenges to grid operators. One of the main issues expected to affect the operation of the power grid is the impact of inverter-based technologies to the power system inertia and, hence, to system stability. Consequently, the main challenge of the future grid is the evaluation of the frequency stability in the presence of inverter-based systems and how the aforementioned technology can support frequency stability without the help of the rotating masses of the traditional power grid systems. To assess the above problem, this paper proposes a methodology to evaluate the frequency stability in a projection of the real distribution grid in Cyprus with the time horizon to be the year 2030. The power grid under investigation is evaluated with and without the presence of smart hierarchical controllers for providing support to the power system under disturbance conditions. The advanced controllers were applied to manage the available power resource in a fast and effective manner to maintain frequency within nominal levels. The controllers have been implemented in two hierarchical levels revealing useful responses for managing low-inertia networks. The first is set to act locally within a preselected area and the second level effectively supporting the different areas for optimal operation. After undertaking a significant number of simulations for time-series of one year, it was concluded from the results that the local control approach manages to minimize the frequency excursion effectively and influence all related attributes including the rate of change of frequency (RoCoF), frequency nadir and frequency zenith.
An Updated Survey of Efficient Hardware Architectures for Accelerating Deep Convolutional Neural Networks
Authors: Capra Maurizio , Bussolino Beatrice , Marchisio Alberto , Shafique Muhammad , Masera Guido , Martina Maurizio
Published: July 2020

Deep Neural Networks (DNNs) are nowadays a common practice in most of the Artificial Intelligence (AI) applications. Their ability to go beyond human precision has made these networks a milestone in the history of AI. However, while on the one hand they present cutting edge performance, on the other hand they require enormous computing power. For this reason, numerous optimization techniques at the hardware and software level, and specialized architectures, have been developed to process these models with high performance and power/energy efficiency without affecting their accuracy. In the past, multiple surveys have been reported to provide an overview of different architectures and optimization techniques for efficient execution of Deep Learning (DL) algorithms. This work aims at providing an up-to-date survey, especially covering the prominent works from the last 3 years of the hardware architectures research for DNNs. In this paper, the reader will first understand what a hardware accelerator is, and what are its main components, followed by the latest techniques in the field of dataflow, reconfigurability, variable bit-width, and sparsity.
Epigenetic Basis of Lead-Induced Neurological Disorders
Authors: Wang Tian , Zhang Jie , Xu Yi
Published: July 2020

Environmental lead (Pb) exposure is closely associated with pathogenesis of a range of neurological disorders, including Alzheimer’s disease (AD), Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS), attention deficit/hyperactivity disorder (ADHD), etc. Epigenetic machinery modulates neural development and activities, while faulty epigenetic regulation contributes to the diverse forms of CNS (central nervous system) abnormalities and diseases. As a potent epigenetic modifier, lead is thought to cause neurological disorders through modulating epigenetic mechanisms. Specifically, increasing evidence linked aberrant DNA methylations, histone modifications as well as ncRNAs (non-coding RNAs) with AD cases, among which circRNA (circular RNA) stands out as a new and promising field for association studies. In 23-year-old primates with developmental lead treatment, Zawia group discovered a variety of epigenetic changes relating to AD pathogenesis. This is a direct evidence implicating epigenetic basis in lead-induced AD animals with an entire lifespan. Additionally, some epigenetic molecules associated with AD etiology were also known to respond to chronic lead exposure in comparable disease models, indicating potentially interlaced mechanisms with respect to the studied neurotoxic and pathological events. Of note, epigenetic molecules acted via globally or selectively influencing the expression of disease-related genes. Compared to AD, the association of lead exposure with other neurological disorders were primarily supported by epidemiological survey, with fewer reports connecting epigenetic regulators with lead-induced pathogenesis. Some pharmaceuticals, such as HDAC (histone deacetylase) inhibitors and DNA methylation inhibitors, were developed to deal with CNS disease by targeting epigenetic components. Still, understandings are insufficient regarding the cause–consequence relations of epigenetic factors and neurological illness. Therefore, clear evidence should be provided in future investigations to address detailed roles of novel epigenetic factors in lead-induced neurological disorders, and efforts of developing specific epigenetic therapeutics should be appraised.
Assessment of the Levels of Oxidative Stress, Muscle Damage, and Psychomotor Abilities of Special Force Soldiers during Military Survival Training
Authors: Różański Paweł , Jówko Ewa , Tomczak Andrzej
Published: July 2020

The aim of this study was to analyze the changes in biochemical markers of oxidative stress and muscle damage, as well as psychomotor abilities during a military survival training. The study included 15 soldiers of special unit (SU), that completed 48 h military survival training combined with sleep deprivation. Before the training (P1), after 24 h (P2), and after 48 h of training (P3), blood samples were taken to measure biochemical markers. At the same time points, the measurements of divided attention and handgrip strength were conducted. Glutathione peroxidase activity decreased significantly at P3, in comparison with P1 and P2 (p < 0.0001), however, no changes were observed in other biochemical markers (i.e., lipid hydroperoxides, creatine kinase and superoxide dismutase activity) throughout the survival training (p > 0.05). The divided attention index was improved significantly at P2 and P3, as compared to P1 (p < 0.05). A tendency to change in maximum strength was found during the training period (main time effect; p = 0.08). Moreover, the strength differentiation (i.e., 50% maximum strength; 50%max) was higher at P3 than at P1 and P2 (p < 0.05). In conclusion, the 48 h survival training in the SU soldiers does not cause oxidative stress or muscle tissue damage, as well as any deterioration, and even improvement in psychomotor abilities. However, the change in strength differentiation (i.e., the production above 60%max instead of target 50%max) after the training may point to deterioration in motor control. Although it should be confirmed in further study with a more numerous group of soldiers, our findings indicate that the special unit soldiers will be able to perform, in a correct manner, specialized tasks related to their long-term activities, especially those which require divided attention. However, participation in long-term survival training, even with low workload, combined with sleep deprivation, results in a deterioration in motor control which may indicate the relevance of monitoring coordination motor abilities/skills in the training process of special unit soldiers.
Differences in Sense of Belonging, Pride, and Mental Health in the Daegu Metropolitan Region due to COVID-19: Comparison between the Presence and Absence of National Disaster Relief Fund
Authors: Kim Young-Jae , Cho Jeong-Hyung , Kim E-Sack
Published: July 2020

Korea’s Daegu Metropolitan City once had the second highest rate of COVID-19 infection after Wuhan in China. Following the outbreak, the government provided the first national disaster relief fund to citizens as financial aid. This study investigated whether the sense of regional belonging, pride, and mental health among 550 citizens of Daegu differed between the times before and after COVID-19, based on the presence or absence of the disaster relief fund. Frequency analysis, descriptive statistical analysis, and t-tests were conducted using the SPSS 25.0 program. Results showed that the sense of belonging was higher after COVID-19 than before, while pride was lower. Individuals who received the disaster relief fund showed higher levels of regional belonging and pride with statistical significance. The prevalence of melancholy and depression increased after COVID-19, but the presence or absence of the fund did not lead to a significant difference. Thus, in case of a future national disaster level, provision of the disaster relief fund can raise the sense of regional belonging and pride, in order to elicit communication among local residents toward overcoming difficulties. Furthermore, during challenging disaster situations, central and local governments should provide diverse programs for the citizens’ mental health care.
Indocyanine Green Loaded Modified Mesoporous Silica Nanoparticles as an Effective Photothermal Nanoplatform
Authors: Wang Yiyu , Niu Chunqing , Fan Sisi , Li Yuwei , Li Xiang , Dai Yujun , Shi Jian , Wang Xinyu
Published: July 2020

Photothermal therapy possesses great advantages for the treatment of drug-resistant tumors. Herein, Near Infrared (NIR)-triggered photothermal nanoparticles were developed through loading indocyanine green (ICG), a kind of NIR dye, into amino group-modified silica nanoparticles (SiO2-NH2 NPs). SiO2-NH2 NPs were prepared with immobilization of the amino groups into the framework of silica nanoparticles (SiO2 NPs) by employing (3-aminopropyl)-triethoxysilane (APTES). Before and after the modification of the amino group, the particle sizes of SiO2 NPs showed similar value, around 100 nm. ICG was further adsorbed into SiO2-NH2 NPs by electrostatic attraction to enable SiO2-NH2@ICG NPs as a kind of photothermal agent. The loading rate of ICG to SiO2-NH2 was greatly increased compared to unmodified SiO2, and the stability of ICG was also improved. Moreover, the SiO2-NH2@ICG NPs exhibited efficient photothermal effects due to ICG transforming laser power into local heat through the connected ICG, when NIR laser irradiation turned on for a couple of minutes. Finally, the in vitro antitumor efficacy of SiO2-NH2@ICG NPs was investigated by recording cell proliferation rate and further chronicled the apoptotic morphology evidence by a Calcein-AM/PI fluorescent staining assay, indicating the efficient photothermal targeted therapy for the HepG2 tumor cells.
Functional Markers for Precision Plant Breeding
Authors: Salgotra Romesh K. , Stewart C. Neal
Published: July 2020

Advances in molecular biology including genomics, high-throughput sequencing, and genome editing enable increasingly faster and more precise cultivar development. Identifying genes and functional markers (FMs) that are highly associated with plant phenotypic variation is a grand challenge. Functional genomics approaches such as transcriptomics, targeting induced local lesions in genomes (TILLING), homologous recombinant (HR), association mapping, and allele mining are all strategies to identify FMs for breeding goals, such as agronomic traits and biotic and abiotic stress resistance. The advantage of FMs over other markers used in plant breeding is the close genomic association of an FM with a phenotype. Thereby, FMs may facilitate the direct selection of genes associated with phenotypic traits, which serves to increase selection efficiencies to develop varieties. Herein, we review the latest methods in FM development and how FMs are being used in precision breeding for agronomic and quality traits as well as in breeding for biotic and abiotic stress resistance using marker assisted selection (MAS) methods. In summary, this article describes the use of FMs in breeding for development of elite crop cultivars to enhance global food security goals.
Transcriptomic Profile of Primary Culture of Skeletal Muscle Cells Isolated from Semitendinosus Muscle of Beef and Dairy Bulls
Authors: Ciecierska Anna , Motyl Tomasz , Sadkowski Tomasz
Published: July 2020

The aim of the study was to identify differences in the transcriptomic profiles of primary muscle cell cultures derived from the semitendinosus muscle of bulls of beef breeds (Limousin (LIM) and Hereford (HER)) and a dairy breed (Holstein-Friesian (HF)) (n = 4 for each breed). Finding a common expression pattern for proliferating cells may point to such an early orientation of the cattle beef phenotype at the transcriptome level of unfused myogenic cells. To check this hypothesis, microarray analyses were performed. The analysis revealed 825 upregulated and 1300 downregulated transcripts similar in both beef breeds (LIM and HER) and significantly different when compared with the dairy breed (HF) used as a reference. Ontological analyses showed that the largest group of genes were involved in muscle organ development. Muscle cells of beef breeds showed higher expression of genes involved in myogenesis (including erbb-3, myf5, myog, des, igf-1, tgfb2) and those encoding proteins comprising the contractile apparatus (acta1, actc1, myh3, myh11, myl1, myl2, myl4, tpm1, tnnt2, tnnc1). The obtained results confirmed our hypothesis that the expression profile of several groups of genes is common in beef breeds at the level of proliferating satellite cells but differs from that observed in typical dairy breeds.
Special Issue “Molecular and Cellular Mechanisms of Preeclampsia”
Authors: Huppertz Berthold
Published: July 2020

Over the last few decades, massive research efforts have been put into deciphering the etiology of the pregnancy pathology preeclampsia [...]
Robust and Scalable Angiogenesis Assay of Perfused 3D Human iPSC-Derived Endothelium for Anti-Angiogenic Drug Screening
Authors: van Duinen Vincent , Stam Wendy , Mulder Eva , Famili Farbod , Reijerkerk Arie , Vulto Paul , Hankemeier Thomas , van Zonneveld Anton Jan
Published: July 2020

To advance pre-clinical vascular drug research, in vitro assays are needed that closely mimic the process of angiogenesis in vivo. Such assays should combine physiological relevant culture conditions with robustness and scalability to enable drug screening. We developed a perfused 3D angiogenesis assay that includes endothelial cells (ECs) from induced pluripotent stem cells (iPSC) and assessed its performance and suitability for anti-angiogenic drug screening. Angiogenic sprouting was compared with primary ECs and showed that the microvessels from iPSC-EC exhibit similar sprouting behavior, including tip cell formation, directional sprouting and lumen formation. Inhibition with sunitinib, a clinically used vascular endothelial growth factor (VEGF) receptor type 2 inhibitor, and 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO), a transient glycolysis inhibitor, both significantly reduced the sprouting of both iPSC-ECs and primary ECs, supporting that both cell types show VEGF gradient-driven angiogenic sprouting. The assay performance was quantified for sunitinib, yielding a minimal signal window of 11 and Z-factor of at least 0.75, both meeting the criteria to be used as screening assay. In conclusion, we have developed a robust and scalable assay that includes physiological relevant culture conditions and is amenable to screening of anti-angiogenic compounds.
Modelling Epithelial Ovarian Cancer in Mice: Classical and Emerging Approaches
Authors: Zakarya Razia , Howell Viive M. , Colvin Emily K.
Published: July 2020

High-grade serous epithelial ovarian cancer (HGSC) is the most aggressive subtype of epithelial ovarian cancer. The identification of germline and somatic mutations along with genomic information unveiled by The Cancer Genome Atlas (TCGA) and other studies has laid the foundation for establishing preclinical models with high fidelity to the molecular features of HGSC. Notwithstanding such progress, the field of HGSC research still lacks a model that is both robust and widely accessible. In this review, we discuss the recent advancements and utility of HGSC genetically engineered mouse models (GEMMs) to date. Further analysis and critique on alternative approaches to modelling HGSC considers technological advancements in somatic gene editing and modelling prototypic organs, capable of tumorigenesis, on a chip.
A Dipeptidyl Peptidase-4 Inhibitor Inhibits Foam Cell Formation of Macrophages in Type 1 Diabetes via Suppression of CD36 and ACAT-1 Expression
Authors: Terasaki Michishige , Yashima Hironori , Mori Yusaku , Saito Tomomi , Matsui Takanori , Hiromura Munenori , Kushima Hideki , Osaka Naoya , Ohara Makoto , Fukui Tomoyasu , Hirano Tsutomu , Yamagishi Sho-ichi
Published: July 2020

Dipeptidyl peptidase-4 (DPP-4) inhibitors have been reported to play a protective role against atherosclerosis in both animal models and patients with type 2 diabetes (T2D). However, since T2D is associated with dyslipidemia, hypertension and insulin resistance, part of which are ameliorated by DPP-4 inhibitors, it remains unclear whether DPP-4 inhibitors could have anti-atherosclerotic properties directly by attenuating the harmful effects of hyperglycemia. Therefore, we examined whether a DPP-4 inhibitor, teneligliptin, could suppress oxidized low-density lipoprotein (ox-LDL) uptake, foam cell formation, CD36 and acyl-coenzyme A: cholesterol acyltransferase-1 (ACAT-1) gene expression of macrophages isolated from streptozotocin-induced type 1 diabetes (T1D) mice and T1D patients as well as advanced glycation end product (AGE)-exposed mouse peritoneal macrophages and THP-1 cells. Foam cell formation, CD36 and ACAT-1 gene expression of macrophages derived from T1D mice or patients increased compared with those from non-diabetic controls, all of which were inhibited by 10 nmol/L teneligliptin. AGEs mimicked the effects of T1D; teneligliptin attenuated all the deleterious effects of AGEs in mouse macrophages and THP-1 cells. Our present findings suggest that teneligliptin may inhibit foam cell formation of macrophages in T1D via suppression of CD36 and ACAT-1 gene expression partly by attenuating the harmful effects of AGEs.
Heat Transfer Enhancement of Impingement Cooling by Adopting Circular-Ribs or Vortex Generators in the Wall Jet Region of A Round Impingement Jet
Authors: Takeishi Ken-Ichiro , Krewinkel Robert , Oda Yutaka , Ichikawa Yuichi
Published: July 2020

In the near future, when designing and using Double Wall Airfoils, which will be manufactured by 3D printers, the positional relationship between the impingement cooling nozzle and the heat transfer enhancement ribs on the target plate naturally becomes more accurate. Taking these circumstances into account, an experimental study was conducted to enhance the heat transfer of the wall jet region of a round impingement jet cooling system. This was done by installing circular ribs or vortex generators (VGs) in the impingement cooling wall jet region. The local heat transfer coefficient was measured using the naphthalene sublimation method, which utilizes the analogy between heat and mass transfer. As a result, it was clarified that, within the ranges of geometries and Reynolds numbers at which the experiments were conducted, it is possible to improve the averaged Nusselt number Nu up to 21% for circular ribs and up to 51% for VGs.
The Birth of the Journal of Molecular Pathology
Authors: Troncone Giancarlo
Published: July 2020

In the era of personalized/precision medicine, the relevance of molecular pathology cannot be overemphasized [...]
Stiffness Prediction of Connections between CHS Tubes and Externally Welded I-Beams: FE Analyses and Analytical Study
Authors: Di Benedetto Sabatino , Latour Massimo , Rizzano Gianvittorio
Published: July 2020

Double-tee profiles are the most popular members in Europe and the USA for steel structures. However, more efficient cross-sections, such as circular hollow sections (CHSs), could be adopted, since they can provide higher aesthetic, economic and mechanical benefits, with the only drawback of more complex connections such as in the case of I-beams welded to the external surface of circular hollow profiles. Based on the ring model theory, developed by Togo, a rule to design the flexural resistance of such a connection has been included in the Eurocode 3 part 1.8, while no formulations are provided to predict the corresponding initial stiffness. The present work aims at filling this knowledge gap, adopting an approach based on experimental, numerical and analytical work. A monotonic and a cyclic test have been performed on two beam-to-column sub-assemblies; the experimental outcomes have been exploited to validate a finite element (FE) model developed in Abaqus and used to numerically perform the monotonic loading simulations of 30 joints. Afterwards, starting from the extracted information about stiffness, a regression analysis was carried out to define the coefficient of a design equation analytically derived applying the component method approach. The regression analysis is characterized by a coefficient of variation equal to 0.19.
Effective 1D Time-Dependent Schrödinger Equations for 3D Geometrically Correlated Systems
Authors: Pandey Devashish , Oriols Xavier , Albareda Guillermo
Published: July 2020

The so-called Born–Huang ansatz is a fundamental tool in the context of ab-initio molecular dynamics, viz., it allows effectively separating fast and slow degrees of freedom and thus treating electrons and nuclei with different mathematical footings. Here, we consider the use of a Born–Huang-like expansion of the three-dimensional time-dependent Schrödinger equation to separate transport and confinement degrees of freedom in electron transport problems that involve geometrical constrictions. The resulting scheme consists of an eigenstate problem for the confinement degrees of freedom (in the transverse direction) whose solution constitutes the input for the propagation of a set of coupled one-dimensional equations of motion for the transport degree of freedom (in the longitudinal direction). This technique achieves quantitative accuracy using an order less computational resources than the full dimensional simulation for a typical two-dimensional geometrical constriction and upto three orders for three-dimensional constriction.
Compositional Variations of Cr-Spinel in High-Mg Intrusions of the Primorsky Ridge (Western Baikal Region, Russia)
Authors: Mekhonoshin Aleksey S. , Kolotilina Tatiana B. , Doroshkov Artemy A. , Pikiner Evgeniya E.
Published: July 2020

Composition variations of Cr-spinel in high-Mg rocks of the Primorsky Ridge (Western Baikal region, Russia) are reported here. A specific feature of Cr-spinels in ultramafic rocks of the Primorsky Ridge is their noticeably high Ti content (up to 6.5 wt.%) compared to spinels in mantle peridotites. The presence of high TiO2 content in Cr-spinels enclosed in olivine crystals may be a clear indication of the primary magmatic nature of Ti enrichment. Two types of Cr-spinel were identified in ultramafic rocks from all intrusions. Cr-spinels of Type I are enclosed in the inner part of olivine crystals and are homogeneous Al-rich chromites and Fe2+-rich chromites. They are characterized by variable content of TiO2 (1.0–5.3 wt.%), moderately high Cr# (0.7–0.83), and low Fe3+# (0.20–0.34). Cr-spinels of type II occur in the interstitial space and occur as homogeneous and zoned grains with Al-rich chromite and Fe2+-rich chromite cores. Al-rich chromite cores have a composition similar to that of the Cr-spinel enclosed in olivine crystals. Fe2+-rich chromite cores have relatively high MgO (3.8–6.2 wt.%), Al2O3 (8–9 wt.%), and TiO2 (2.6–2.8 wt.%) content, low MnO (0.34–0.52 wt.%) content, and a low Fe3+# (0.25–0.27) ratio.
Effect of a =X-NH-Fragment, (X = C, N), on Z/E Isomerization and ON/OFF Functionality of Isatin Arylhydrazones, ((Arylamino)Methylene)Indolin-2-Ones and Their Anions
Authors: Tisovský Pavol , Csicsai Klaudia , Donovalová Jana , Šandrik Róbert , Sokolík Róbert , Gáplovský Anton
Published: July 2020

The subject of this work was the study of thermally and photochemically stimulated Z ↔ E isomerization and hydrazo ↔ azo tautomerism of Z- and E-isomers of isatin arylhydrazones and ((arylamino)methylene)indolin-2-ones and their anions. Using NMR, UV-Vis spectroscopy, kinetic measurements, and HPLC, we studied the relationship of structure, (Z- and E-isomers), of these compounds and hydrazo=azo tautomerism. The ON/OFF functionality of these compounds and their anions using light to stimulate switching between ON and OFF states was investigated. We pointed out the characterization of the effect of =N- and =CH- structural fragments and aryl structure on ON and OFF states of isatin arylhydrazones and ((arylamino)methylene)indolin-2-ones.
The GABAB Receptor—Structure, Ligand Binding and Drug Development
Authors: Evenseth Linn Samira Mari , Gabrielsen Mari , Sylte Ingebrigt
Published: July 2020

The γ-aminobutyric acid (GABA) type B receptor (GABAB-R) belongs to class C of the G-protein coupled receptors (GPCRs). Together with the GABAA receptor, the receptor mediates the neurotransmission of GABA, the main inhibitory neurotransmitter in the central nervous system (CNS). In recent decades, the receptor has been extensively studied with the intention being to understand pathophysiological roles, structural mechanisms and develop drugs. The dysfunction of the receptor is linked to a broad variety of disorders, including anxiety, depression, alcohol addiction, memory and cancer. Despite extensive efforts, few compounds are known to target the receptor, and only the agonist baclofen is approved for clinical use. The receptor is a mandatory heterodimer of the GABAB1 and GABAB2 subunits, and each subunit is composed of an extracellular Venus Flytrap domain (VFT) and a transmembrane domain of seven α-helices (7TM domain). In this review, we briefly present the existing knowledge about the receptor structure, activation and compounds targeting the receptor, emphasizing the role of the receptor in previous and future drug design and discovery efforts.
Thiyl Radicals: Versatile Reactive Intermediates for Cyclization of Unsaturated Substrates
Authors: Lynch Dylan M. , Scanlan Eoin M.
Published: July 2020

Sulfur centered radicals are widely employed in chemical synthesis, in particular for alkene and alkyne hydrothiolation towards thioether bioconjugates. The steadfast radical chain process that enables efficient hydrothiolation has been explored in the context of cascade reactions to furnish complex molecular architectures. The use of thiyl radicals offers a much cheaper and less toxic alternative to the archetypal organotin-based radical methods. This review outlines the development of thiyl radicals as reactive intermediates for initiating carbocyclization cascades. Key developments in cascade cyclization methodology are presented and applications for natural product synthesis are discussed. The review provides a chronological account of the field, beginning in the early seventies up to very recent examples; a span of almost 50 years.
Adsorption Capacity of Vitamin B12 and Creatinine on Highly-Mesoporous Activated Carbons Obtained from Lignocellulosic Raw Materials
Authors: Lupaşcu Tudor , Petuhov Oleg , Ţîmbaliuc Nina , Cibotaru Silvia , Rotaru Andrei
Published: July 2020

Enterosorbents are widely-used materials for human body detoxification, which function by immobilizing and eliminating endogenous and exogenous toxins. Here, activated carbons, obtained from the lignocellulosic raw vegetal materials of indigenous provenance, have been studied. Walnut shell and wood from local species of nuts and apple-trees were carbonized, and further activated at high temperatures with water vapors in a rotary kiln. A second activation was carried out, in a fluidized bed reactor, but for shorter times. The textural properties of the samples were determined from the adsorption isotherms of nitrogen at 77 K, allowing the obtaining of highly mesoporous materials, while the adsorption capacity permitted an essential rise of six to seven times in the maximal adsorption values of the metabolites, which was determined by the reactivation process. A kinetic study of vitamin B12 and creatinine immobilization was performed, the optimal immobilization time for the apple-tree wood reactivated carbons being 2 times longer than for those originating from walnut shells. An additional investigation was also performed in specific conditions that simulate the real environment of immobilization: the temperature of a febrile human body (at the temperature T = 38 °C) and the characteristic acidity of the urinary tract and stomach (at the pH of 5.68 and 2.53, respectively). The activated carbonic adsorbents studied here, together with the results of the immobilization studies, show that these procedures can conduct a good incorporation of some endogenous metabolic products, such as vitamin B12 and creatinine, therefore presenting a good opportunity for their use as forthcoming commercial enterosorbents.
Anticancer Potential and Capsianosides Identification in Lipophilic Fraction of Sweet Pepper (Capsicum annuum L.)
Authors: Chilczuk Barbara , Marciniak Beata , Stochmal Anna , Pecio Łukasz , Kontek Renata , Jackowska Izabella , Materska Małgorzata
Published: July 2020

This study aimed to determine the health-promoting properties of sweet pepper by comparing the activity of fractions with variable lipophilicity. Fractions from red pericarp: aqueous (F1), 40% MeOH (F2), and 70% MeOH (F3) were analyzed for antiradical activity (with DPPH• and ABTS+•), and the contents of total phenolic compounds (TP), flavonoids (TF), and dihydroxycinnamic acids (TDHCA). The anticancer potential of the fractions was evaluated in vitro using different cancer cell lines: human colorectal carcinoma (HCT116) and PC-3 (prostate cancer cell). Fibroblast-like cells of L929 obtained from subcutaneous adipose tissue of mouse were used as normal cells. The highest content of TP, TF, and TDHCA along with the strongest antiradical activity was observed for fraction F2, while the strongest anticancer properties against PC-3 were observed in fraction F3. Fraction F3 primarily contained capsianoside derivatives, which had been isolated through chromatographic methods and identified by spectral methods. These analyses helped in identifying 8 compounds, including 3 new compounds.
Unravelling the Potential of Salivary Volatile Metabolites in Oral Diseases. A Review
Authors: Pereira Jorge A. M. , Porto-Figueira Priscilla , Taware Ravindra , Sukul Pritam , Rapole Srikanth , Câmara José S.
Published: July 2020

Fostered by the advances in the instrumental and analytical fields, in recent years the analysis of volatile organic compounds (VOCs) has emerged as a new frontier in medical diagnostics. VOCs analysis is a non-invasive, rapid and inexpensive strategy with promising potential in clinical diagnostic procedures. Since cellular metabolism is altered by diseases, the resulting metabolic effects on VOCs may serve as biomarkers for any given pathophysiologic condition. Human VOCs are released from biomatrices such as saliva, urine, skin emanations and exhaled breath and are derived from many metabolic pathways. In this review, the potential of VOCs present in saliva will be explored as a monitoring tool for several oral diseases, including gingivitis and periodontal disease, dental caries, and oral cancer. Moreover, the analytical state-of-the-art for salivary volatomics, e.g., the most common extraction techniques along with the current challenges and future perspectives will be addressed unequivocally.
The Inhibitory Activity of Anthraquinones against Pathogenic Protozoa, Bacteria, and Fungi and the Relationship to Structure
Authors: Friedman Mendel , Xu Alexander , Lee Rani , Nguyen Daniel N. , Phan Tina A. , Hamada Sabrina M. , Panchel Rima , Tam Christina C. , Kim Jong H. , Cheng Luisa W. , Land Kirkwood M.
Published: July 2020

Plant-derived anthraquinones were evaluated in cell assays for their inhibitory activities against the parasitic protozoa Trichomonas vaginalis human strain G3 that causes the sexually transmitted disease trichomoniasis in women, Tritrichomonas foetus bovine strain D1 that causes sexually transmitted diseases in farm animals (bulls, cows, and pigs), Tritrichomonas foetus-like strain C1 that causes diarrhea in domestic animals (cats and dogs), and bacteria and fungi. The anthraquinones assessed for their inhibitory activity were anthraquinone, aloe-emodin (1,8-dihydroxy-3-hydroxymethylanthraquinone), anthrarufin (1,5-dihydroxyanthraquinone), chrysazin (1,8-dihydroxyanthraquinone), emodin (1,3,8-trihydroxy-6-methylanthraquinone), purpurin (1,2,4-trihydroxyanthraquinone), and rhein (1,8-dihydroxy-3-carboxyanthraquinone). Their activities were determined in terms of IC50 values, defined as the concentration that inhibits 50% of the cells under the test conditions and calculated from linear dose response plots for the parasitic protozoa, and zone of inhibition for bacteria and fungi, respectively. The results show that the different substituents on the anthraquinone ring seem to influence the relative potency. Analysis of the structure–activity relationships in protozoa indicates that the aloe-emodin and chrysazin with the highest biological activities merit further study for their potential to help treat the diseases in women and domestic and farm animals. Emodin also exhibited antifungal activity against Candida albicans. The suggested mechanism of action and the additional reported beneficial biological properties of anthraquinones suggest that they have the potential to ameliorate a broad spectrum of human diseases.
Cook-EdTM: A Model for Planning, Implementing and Evaluating Cooking Programs to Improve Diet and Health
Authors: Asher Roberta C. , Jakstas Tammie , Wolfson Julia A. , Rose Anna J. , Bucher Tamara , Lavelle Fiona , Dean Moira , Duncanson Kerith , Innes Beth , Burrows Tracy , Collins Clare E. , Shrewsbury Vanessa A.
Published: July 2020

Domestic cooking education programs are typically designed to improve an individual’s food and cooking skills, although not necessarily diet quality. Currently, there are no comprehensive models to guide the planning, implementation and evaluation of domestic cooking education programs that focus on improving diet and health. Our aim was to address this through development of the Cooking Education (“Cook-EdTM”) model, using the PRECEDE-PROCEED model as the underlying Cook-EdTM framework. A review of the food and cooking skills education literature informed the content of the Cook-EdTM model. Cook-EdTM was critiqued by experts in consumer behaviour, cooking and nutrition education research and education until consensus on model content and format was reached. Cook-EdTM leads cooking program developers through eight distinct stages, engaging key stakeholders in a co-design process from the outset to tailor programs to address the need of individuals and inform the development of program content, program delivery, and evaluation. A Cook-EdTM scenario applied in practice is described. The proposed Cook-EdTM model has potential to be adapted for use in domestic cooking education programs delivered in clinical, community, school or research settings. Further research will establish Cook-EdTM’s utility in enhancing program development and in improving food and cooking skills, dietary patterns and health outcomes.
Dietary Strategies for Maintenance of Clinical Remission in Inflammatory Bowel Diseases: Are We There Yet?
Authors: Gkikas Konstantinos , Gerasimidis Konstantinos , Milling Simon , Ijaz Umer Z. , Hansen Richard , Russell Richard K.
Published: July 2020

The etiopathogenesis of Inflammatory bowel disease (IBD) is a result of a complex interaction between host immune response, the gut microbiome and environmental factors, such as diet. Although scientific advances, with the use of biological medications, have revolutionized IBD treatment, the challenge for maintaining clinical remission and delaying clinical relapse is still present. As exclusive enteral nutrition has become a well-established treatment for the induction of remission in pediatric Crohn’s disease, the scientific interest regarding diet in IBD is now focused on the development of follow-on dietary strategies, which aim to suppress colonic inflammation and delay a disease flare. The objective of this review is to present an extensive overview of the dietary strategies, which have been used in the literature to maintain clinical remission in both Crohn’s disease and Ulcerative colitis, and the evidence surrounding the association of dietary components with clinical relapse. We also aim to provide study-related recommendations to be encompassed in future research studies aiming to investigate the role of diet during remission periods in IBD.