bluuebunny/crossref_metadata_2025 huggingface dataset:
Gestão dos Colegiados e Governança: ação coletiva e transição do Colegiado nos territórios Açu-Mossoró e Sertão do Apodi (RN)
Authors: Nunes Emanoel Márcio , SILVA Márcia Regina Farias da , SÁ Vinícius Claudino de , GONDIM Maria de Fátima Rocha
Published: April 2024

O objetivo é analisar a contribuição da Gestão dos Colegiados territoriais, considerando a governança e a importância da ação coletiva em processos de dinamização e transição para a sustentabilidade dos territórios Açu-Mossoró e Sertão do Apodi, no Rio Grande do Norte. Como metodologia, foram aplicados 77 questionários junto à plenária do colegiado territorial, visando obter características que definem sua identidade, e sobre as estratégias de gestão na definição de ações e projetos que impulsionam o desenvolvimento territorial. Conclui-se, que é imprescindível pensar numa gestão do colegiado que fortaleça a tomada de decisão, com a finalidade da transição dos colegiados para a sustentabilidade. E é justamente o que vem a se constituir um dos grandes desafios, no sentido de melhorar as estruturas de governança (sociedade civil e governos municipal, estadual e federal), para a efetivação de estratégias de inclusão que venham a integrar a agricultura familiar organizada à agroindústria familiar e a construir e ampliar mercados locais e regionais.
Programa Nacional de Fortalecimento da Agricultura Familiar: uma análise da distribuição regional dos recursos no Noroeste do Rio Grande do Sul
Authors: Endruweit Battisti Iara Denise , Writzl Darlan Nei , Rotta Edemar
Published: April 2024

O Programa Nacional de Fortalecimento da Agricultura Familiar (Pronaf) é o principal programa do governo federal de apoio à agricultura familiar. Por isso, é importante avaliar esta política pública, para verificar se, apesar dos valores elevados concedidos, consegue atender a um grupo diverso de beneficiários. O objetivo é identificar fatores que influenciam a distribuição de recursos do Pronaf em municípios que integram uma das nove Regiões Funcionais de Planejamento do Rio Grande do Sul (RS), a RF7, composta pelos COREDEs Celeiro, Noroeste Colonial, Fronteira Noroeste e Missões. Trata-se de uma pesquisa com abordagem quantitativa com alcance correlacional. Os dados foram coletados em bases públicas - Instituto Brasileiro de Geografia e Estatística (IBGE) e Banco Central do Brasil (BCB), para o ano de 2017. Para a análise fez-se uso de técnicas estatísticas univariada e bivariada no software estatístico R. Os resultados demonstram que há uma correlação direta dos recursos acessados por meio do Pronaf nos municípios da RF7 com a quantidade de commodities (soja, milho e trigo) produzidas pela agricultura familiar, o número de tratores per capita e o percentual de estabelecimentos que utilizam agrotóxicos. Há correlação indireta entre o percentual de estabelecimentos classificados como Pronaf B (menor renda) e o percentual de estabelecimentos dirigidos por mulheres. Além disso, não foi verificada relação com o percentual de estabelecimentos dirigidos por pessoas com menos de 25 anos. Espera-se que, com este estudo, os formuladores de políticas públicas consigam detectar algumas fragilidades do Pronaf e aplicar melhorias para atender à heterogeneidade da agricultura familiar.
Walt Whitman. Leaves of Grass / Grashalme: Zweisprachige Fassung der Erstausgabe von 1855
Authors: Schöberlein Stefan
Published: April 2024

Walt Whitman. Leaves of Grass / Grashalme: Zweisprachige Fassung der Erstausgabe von 1855. Translated by Walter Grünzweig and a team of translators at TU Dortmund University. Aachen: Rimbaud, 2022. 226 pp. 
Walt Whitman: A Current Bibliography, Summer/Fall 2023
Authors: Folsom Ed
Published: April 2024

Walt Whitman: A Current Bibliography, Summer/Fall 2023
Efectos del reemplazo parcial de harina de trigo con harina de banano verde sobre las propiedades reológicas de la masa y las propiedades de calidad de pan
Authors: Rodriguez Sandoval Eduardo , Manjarres-Pinzon Gustavo , Castro-Sanchez Adriana , Lopez-Ochoa Juan David , Gil-Gonzalez Jesus
Published: April 2024

Objetivo: Evaluar la sustitución parcial de harina de trigo con harina de banano verde verificando las propiedades de calidad del pan molde. Metodología: Se caracterizó la harina de banano verde en sus propiedades fisicoquímicas, de empastamiento y composicionales. Se evaluaron las propiedades reológicas y farinográficas de las masas, y se determinaron las propiedades fisicoquímicas, texturales, de color y macroestructurales por análisis de imágenes de los panes con sustitución del 5%, 10% y 20% con harina de banano verde. Resultados: La harina de banano verde mostró un alto contenido de almidón y fibra dietaría con valores de 85.1 g/100 g y 8.6 g/ 100g, respectivamente. El incremento en el porcentaje de sustitución con harina de banano de rechazo resultó en mayores valores de G’ y G’’, y menor estabilidad en las masas con respecto al tratamiento control. No se observaron diferencias significativas en las propiedades fisicoquímicas de los panes con los diferentes tratamientos. A mayor sustitución de la harina de trigo por harina de banano (20%) se aumentó la firmeza de la miga, la dureza y la masticabilidad, y disminuyó su cohesividad y resilencia. El color de la miga es más oscuro y con una tonalidad marcada a amarillo a medida que se aumenta la sustitución por harina de banano verde. Conclusiones: La sustitución de harina de trigo por harina de banano verde por encima del 10% puede presentar deficiencias en la calidad del pan molde, siendo más evidente en las  propiedades texturales y de color
Analysis of the extent of heavy vehicle overloading on Namibian trunk roads and evaluation of the effectiveness of existing mitigation measures
Authors: Agoro G , Johannes P , Ambunda R
Published: April 2024

Overloading of heavy vehicles reduces pavement life and increases pavement life cycle costs. As part of Namibia's strategy to control heavy vehicle overloading on the road network, weighbridge facilities have been constructed at strategic locations on primary routes (trunk roads). The study analysed the extent of heavy vehicle overloading on Namibian trunk roads, as well as the effectiveness of existing overloading mitigation measures. The dataset comprised heavy vehicle loading information from the year 2015 to 2019, from ten static weighbridge facilities. The parameters investigated include overloading magnitude, the effect of overloading on road pavement life, and the effectiveness of current overload mitigation measures. The results showed that 13.3% of the vehicles weighed were overloaded, with a compounded decrease in overloading of approximately 0.5% per annum. Despite the overloading decrease, the study found that the estimated road service life was reduced by as much as nine years over the study period. High levels of weighbridge avoidance and poor stakeholder coordination in mitigating overloading were identified. The study recommends deploying high-speed weigh-in-motion systems, an increase in fines charged for overloading offenses, and developing a demerit point-based system for habitual offenders to strengthen mitigation measures.
A comparison of traditional road safety assessment methods and the newly developed 'road safety deserts' approach
Authors: Vanderschuren M J W A , Newlands A G
Published: April 2024

Road fatalities were labelled a pandemic as early as 1973 (BMJ 1973). The number of road fatalities reached 1.35 million in 2016. Currently over 3 500 people perish every day on the world's roads. South Africa has one of the highest road fatality rates in the world, with a fatality rate of 25.9 deaths per 100 000 population (WHO 2018). In order to understand and improve the road safety situation of a region, effective road safety assessments must be carried out. This paper presents a comparison of four different road safety assessment approaches, both traditional and novel, and serves as a proof of concept for the 'road safety desert' methodology, a new technique adapted from the 'transit desert' concept. This new approach to road safety assessment explores the possibility of geo-coded supply and demand comparisons to identify 'road safety deserts' - areas that have a comparatively higher road safety risk. This paper shows that there are several unique and effective ways to assess road safety, and that each approach incorporates different characteristics within their methodologies. It is recommended that road safety analysis is conducted using a multitude of methods, so as to improve understanding and intervention selection.
'Our machines are disturbingly lively, and we ourselves Mghteningly inert': Reconsidering ironic kinship in Neill Blomkamps science fiction film Chappie
Authors: du Plooy Belinda
Published: April 2024

Neill Blomkamp's 2015 science fiction film Chappie engages with the familiar narrative trope of robot sentience. Blomkamp confronts viewers with a naïve and vulnerable childlike robot protagonist that is more human and likeable than any of the stilted and stereotypical real human characters in the film. It is the mechanical creature with which the viewer readily identifies and sympathises. Blomkamp facilitates, not only between his characters, but also with the audience, a kinship of the sort that Donna Haraway in Staying with trouble calls affinity groups or assemblages of 'oddkin'. The immediately sympathetic response of the viewing audience to the mechanical robot is a key strategy in the way that Blomkamp applies irony in this film, which Haraway also identifies as central to her idea of the cyborg as an alternative and potentially liberatory myth. In this article, I engage in a close reading of the film, focusing on the broad network of speculative and science fiction narrative traditions within which this film operates. I consider possible reasons why the film was misread and met with criticism when it was first released. I also specifically investigate the strategies and techniques Blomkamp uses in his depiction of the robot character and how his use of its childlikeness and vulnerability and its engagement with violence and sacrifice are central to the film's ironic engagement with the central argument about the dangers of dehumanisation and the need to recuperate humaneness.
Análise comparativa do comportamento verbal nos três níveis de suporte do autismo
Authors: Gaiato Mayra Helena Bonifácio , Zotesso Marina Cristina , Silveira Rodrigo da Rosa , Ferreira Lidiane
Published: April 2024

OBJETIVO: O presente trabalho teve por objetivo avaliar e comparar a habilidade do comportamento verbal em crianças com distintos níveis de suporte do TEA. MÉTODO: Foram avaliadas onze crianças diagnosticadas com autismo e com faixa etária entre 2 e 7 anos e que apresentassem diversidade entre si quanto ao nível de suporte TEA. Para a averiguação do repertório de comunicação, eles foram avaliados a partir de um instrumento elaborado por uma equipe de profissionais especializados, investigando o comportamento não verbal, ecoico, mando, tato e intraverbal em três tentativas. RESULTADOS: Apesar do número reduzido de participantes, os resultados indicaram que pacientes no nível 3 de suporte apresentam maior comprometimento na comunicação comparado aos demais. O estudo destacou a importância do rastreio de habilidades comportamentais para um planejamento com maior eficácia para a intervenção e concomitantemente evolução clínica, respeitando assim as particularidades e singularidades de cada pessoa no espectro. CONCLUSÃO: Concluiu-se assim, a importância da análise de comportamentos e a investigação detalhada para cada paciente, a fim de que as intervenções sejam focadas em suas reais necessidades.
Management of bipolar disorder at work
Authors: Michel Bourin
Published: April 2024

Bipolar disorder can lead to problems at work, whether in social relationships or the difficulty of certain tasks. The aim of this article is to distinguish between the different challenges that bipolar patients must overcome. Whether it concerns working hours, teamwork, or the choice of profession. Advice is offered to bipolar people to help them in their work, but also what legal protections they are entitled to.
Evaluation of the treatment outcome of tibia shaft fracture by close reduction and internal fixation with interlocking intramedullary nail, Freetown, Sierra Leone, 2023
Authors: Desmond Maada Kangbai , Nella Clemens-Kangbai , Isaac Olufemi Smalle
Published: April 2024

Introduction: The tibia shaft is a common site for bone fractures and open fractures. Tibia shaft fracture is mostly caused by high-energy trauma such as a fall from a height or a road traffic accident. The objective of this study was to evaluate the treatment outcome of tibia shaft fracture managed by close reduction and internal fixation with interlocking Intramedullary Nails (IMN) and to determine factors that are associated with tibia fracture infection. Methods: This retrospective study was conducted at the Emergency Trauma Hospital, Freetown, Sierra Leone. We analyzed the medical records of a mixed cohort of patients who were treated for, tibia shaft fracture by closed reduction and internal fixation with interlocking intramedullary nails for the period January 2020 to January 2022; with at least 15 months of follow-up. We later used both bivariate and multivariate logistic regressions to determine the factors that are associated with wound infection post-internal fixation. Results: A total of Ninety (n = 90) patients’ medical records were reviewed of which 79 were included for analysis. The median age was 30 years (Inter Quartile Range: 16-67 years). The average hospital stay was 20 days (Inter Quartile Range: 6-156 days). The mean fracture nailing time was 11 days. Infection and non-union rates were at 18.99% and 3.8% respectively. Type of tibia shaft fracture (AOR = 25.51, 95% CI = 2.06-422.75,) and characteristics of wound (AOR = 29.51, 95% CI = 2.06-422.75) were significantly associated with wound infection. The treatment outcome categorization of IMN was successful in 88.61% of the patients. Conclusion: This study concludes that the type of tibia shaft fracture and characteristics of the wound are major predictors of wound infection. The findings of this study suggest that IMN fixation of closed and Gustilo type 1 Tibia shaft fracture led to successful healing in the majority of the cases with a low non-union rate.
Nanopore Sequencing of poliovirus isolates v1
Authors: Troman Catherine , Bujaki Erika , Akello Joyce , Fitz Shannon , Shaw Alex , Martin Javier , Grassly Nick
Published: April 2024

This protocol is adapted from "Direct Detection of poliovirus and Nanopore Sequencing (DDNS) - Stool" to make it suitable for use with cell culture isolates in either a liquid form or that have been transported to the lab via FTA card. The protocol aims to amplify the VP1 region of poliovirus through a single PCR using the Q8/Y7 primer set. We use barcoded primers as this greatly simplifies the subsequent library preparation process. Primer sequences for the barcoded Q8/Y7 primers are found in Dataset S1 of Shaw et al (2020). This protocol is for use with Oxford Nanopore v14 chemistry sequencing reagents and can be used with the MinION Mk1B or GridION sequencer. Within the protocol steps, quality control checks are included and follow the workflow set out in the document "Quality Control and Data Recording for DDNS".
Preparation of Free Floating Coronal Mouse Brain Sections v1
Authors: Erb madalynn.erb
Published: April 2024

Protocol to prepare free floating mouse brain sections for immunostaining or in situ hybridization.
BaseScope In Situ Hybridization v1
Authors: Erb madalynn.erb
Published: April 2024

BaseScope in situ hybridization on mouse brain sections
Preparation, Processing and Preservation of Deceased Donor Kidney Tissue for Multiomic Studies v1
Authors: Jain Sanjay , Knoten Amanda , Conlon Kristy
Published: April 2024

Multiomic technologies are increasingly being used on human samples and generating multidimensional data. Preanalytical and tissue procurement factors can have a lasting and negative effect on omic results. For these data to be biologically and clinically useful, it is essential that high quality samples are obtained with rigor and reproducibility. By using kidney tissue procurement as an example, we present an end-to-end detailed pipeline from deceased donor patients from consent to tissue procurement, processing and preservation. This approach has been extensively tested on various atlasi projects and multiple tissue types including the Human Biomolecular Atlas Program (HuBMAP) and the Kidney Precision Medicine Project (KPMP).
Microbe/Phage Wastewater DNA/RNA Concentration and Extraction (Nanotrap® and NucleoMag® RNA Water) v1
Authors: Rasile Brett , Maas Kendra , Anand Archana , Hajkowski Michael
Published: April 2024

Wastewater epidemiology is a method used to study the diseases affecting a population. Conventional methods of wastewater collection for composite samples have involved the usage of autosamplers that require installation in sewers with appropriate expertise and certification. Tampon-based wastewater sampling is a cost-effective and simple way to collect wastewater to study microbial pathogens. It is also a safer alternative as tampons can be deployed and retrieved without opening manhole covers. In this protocol we detail a wastewater sampling strategy using tampons to obtain composite samples. Next, wastewater-based epidemiology has predominantly been targeted, where scientists have looked for specific pathogens and use biomarkers for the same. Notably, traditional wastewater filtration methods (i.e. skim milk filtrations and membrane filtration) require large sample sizes with varying nucleic acid yields (Ahmed 2022). Recently, Nanotrap‱ technology has been shown to have a higher nucleic acid yield using a smaller sample size when compared to traditional membrane filtration techniques, making it a better tool for scientists to concentrate microbial nucleic acids in wastewater for wastewater epidemiology. Specifically, the Ceres Nanotrap‱ technology was developed to trace severe acute respiratory syndrome (SARS-CoV-2) in public wastewater and Nanotrap‱ particles have been used specifically to track viral pathogenic shedding in wastewater of: Endogenous pepper mottle virus (PMMoV), Influenza A, CrAssphage, and monkey pox. However, there is little known about the success rates of different downstream analysis techniques to concentrate microbes in wastewater in an untargeted fashion. Therefore, it remains unclear whether one method will work better than the other if one were to be interested in characterizing the microbial community composition and abundance in a wastewater sample. To this end, in this protocol we detail a wastewater processing method involving Ceres Nanotrap‱ technology and NucleoMag‱ RNA Water.
Nuclei Isolation for Human Ovary Explants v1
Authors: Martin Nicolas
Published: April 2024

This protocol uses fresh frozen human ovary explants to isolate nuclei suspension.
Brain Homogenization and MSD Protocol for Mouse Brain and Serum v1
Authors: Vermilyea Scott
Published: April 2024

This protocol details about the homogenization and MSD protocol of the mouse brain using various panel kits.
The design and manufacture of massively scalable inertial focusing prototype microfluidic devices v1
Authors: Carvell Thomas
Published: April 2024

Microfluidics is a rapidly expanding field and microfluidic devices have been used in a variety of biomedical applications such as cell sorting, disease diagnostics and various lab-on-a-chip systems. There is great demand for manufacturing techniques capable of fabricating ever more intricate microstructures for increasingly complex applications whilst remaining cost-effective for use in biomedical research. Conventional manufacturing techniques can be used to fabricate many complex microchannel architectures but are often expensive, low throughput, have poor microfeature resolution or are unsuitable to be used at scale. To address this issue, we describe a manufacturing technique that employs stereolithography 3D printing to produce a base substrate which can be sealed with a laser patterned adhesive layer cover and stacked in a compact configuration. The technique provides for the design and manufacturing of a device that can bemassively parallelized at relatively low cost and with a small laboratory footprint. This paper aims to fully explain the design and manufacturing process undertaken to allow the use of this technique in future research.
Baiting Pythium myriotylum from Infested Soil v2
Authors: Weerasuriya Nimalka
Published: April 2024

Baiting Pythium from seedlings in infested soil or infected hulls.
Persistence of a new coronavirus infection in a patient with primary central nervous system large B-cell lymphoma with assessment of the humoral immune response against SARS-CoV-2
Authors: Polyakov Yu. Yu. , Baryakh E. A. , Misyurina E. N. , Zhelnova E. I. , Mingalimov M. A. , Kardovskaya S. A. , Smolyarchuk M. Ya. , Tolstykh T. N. , Chudnova T. S. , Ivanova D. D. , Kochneva O. L. , Lebedev D. V. , Abueva A. U. , Chistov A. M. , Zotina E. N. , Samsonova I. V. , Lysenko M. A.
Published: April 2024

Treatment of immunocompromised patients with novel coronavirus infection (COVID-19) presents significant challenges. Currently, there are no unified approaches to the treatment of persistent COVID-19 in hematological malignancies. There is a need to develop recommendations for the management of such patients, chemotherapy protocols, as well as therapy for COVID-19 in case of SARS-CoV-2 virus persistence. Doctors are faced with cases of virus persistence, clinical manifestations during a long course of the infectious process and are not provided with methodological recommendations for patient supervision. As scientific data on the persistent COVID-19 course in patients with lymphoproliferative diseases accumulates, it is planned to create recommendations for the treatment of COVID-19 for patients in this group. This article describes a clinical case of persistent COVID-19 course in a comorbid patient with primary central nervous system large B-cell lymphoma during chemotherapy.
Significance of immunophenotypic, cytogenetic, and molecular markers in adult patients with T-cell lymphoblastic leukemia
Authors: Vasileva A. N. , Aleshina O. A. , Kotova E. S. , Biderman B. V. , Obukhova T. N. , Galtseva I. V. , Dvirnyk V. N. , Zakharko E. I. , Sudarikov A. B. , Parovichnikova E. N.
Published: April 2024

Background. Current chemotherapy protocols for T-cell acute lymphoblastic leukemia (T-ALL) allow achieving a 5-year overall survival of 60–90 %, but relapsed and refractory forms remain incurable situations.

Aim. To determine the significance of immunophenotypic, cytogenetic and molecular markers in adult T-ALL patients receiving therapy according to the ALL-2016 protocol.

Materials and methods. From December 2016 to June 2022, 113 patients with primary T-ALL were included in the study. Cytogenetic study was performed in 104 (92 %) patients; anomalies in the IKZF1 and NOTCH1 genes were investigated in 43 (38 %) patients.

Results. The worst prognosis was in patients with ETP and near-ETP variants of T-ALL (3-year disease-free survival was 54 % in ETP group, 33 % in near-ETP group vs TI/II – 79 %, TIII – 89 %, TIV – 75 %). In early T-ALL variants, abnormal karyotype was most common (ETP – 80.7 %, near-ETP – 60 %). Aberrations in NOTCH1 gene were found in 53 % of cases (in 23 out of 43 patients), and no mutations were found in IKZF1 gene in our study. In the group with no NOTCH1 abnormalities, the overall survival was significantly worse than in the group with abnormalities (NOTCH1– – 52 % vs NOTCH1+ –81 %; p = 0.05).
Resolution on the results of the regional conference “Modern view of the multiple myeloma patient treatment in real clinical practice”. February 21, 2024, Irkutsk
Authors: Editorial Article
Published: April 2024

Во время региональной конференции «Современный взгляд на терапию пациента с множественной миеломой в реальной клинической практике» была затронута важная тема, посвященная болевому синдрому. С докладами о проблемах болевого синдрома выступили Али Мурадович Мудунов, Максим Бокманович Пак и Вадим Евгеньевич Груздев.
Cladribine chemotherapy of multifocal, multisystemic form of Rosai–Dorfman disease: literature review and case report
Authors: Potapenko V. G. , Abramov D. S. , Baykov V. V. , Grigorieva T. L. , Selinkina M. S. , Nikolskaya L. O. , Emile J.-F.
Published: April 2024

Rosai–Dorfman disease is the most frequent variant of non-Langerhans cell histiocytosis. Local forms can be resected or irradiated. If the process involves multiple organs, systemic chemotherapy can cure some patients. This article includes literature review and a case report of a 34-year-old patient with multifocal, multisystemic form of Rosai–Dorfman disease with bone and pleural involvement. The diagnosis was based on histological, immunohistochemical, and molecular studies of tumor tissue. Since November 2021, 6 courses of chemotherapy with cladribine and 8 infusions of zolendronic acid were carried out with achievement of durable remission. The tolerance was acceptable.
Immunophenotyping of blood and bone marrow cells as a way to search for differentiation syndrome risk factors in acute promyelocytic leukemia
Authors: Semenova A. A. , Galtseva I. V. , Troitskaya V. V. , Kapranov N. M. , Davydova Yu. O. , Nikiforova K. A. , Loseva A. G. , Ermolaev A. A. , Surimova V. A. , Kulikov S. M. , Parovichnikova E. N.
Published: April 2024

Background. Differentiation syndrome (DS) is a potentially fatal complication of therapy for acute promyelocytic leukemia (APL) with an incidence of up to 48 %. To date, no reliable DS risk factors have been found, with the exception of leukocytosis at the APL onset.

Aim. To determine the risk factors associated with DS in patients with APL during induction therapy with arsenic trioxide (ATO) and tretinoin (ATRA).

Materials and methods. The study included 39 patients with APL, 29 (74.4 %) of them were classified as low-risk according to ELN (European Leukemia Net), 10 (25.6 %) were classified as high-risk. At the disease onset, cytological and molecular (chimeric transcript PML::RARα, FLT3-ITD mutation) bone marrow studies were performed, the expression of 28 differentiation antigens by blood and bone marrow blast cells was determined (markers of early precursors, myeloid and lymphoid differentiation, cell adhesion molecules, chemokine receptors, integrins, selectin), body mass index (BMI) and the leukocytes number dynamics during induction course were assessed. All patients received ATRA and ATO therapy. Patients from the high-risk group at the onset received 1–3 injections of idarubicin (12 mg/m2) and dexamethasone (8–10 mg/m2 2 times a day) to prevent DS until leukocytosis reduced. In cases of DS, dexamethasone was prescribed at a dose of 10 mg/m2 2 times a day; in cases of severe DS, the induction course was interrupted.

Results. Of the 39 patients, 12 (30.8 %) were diagnosed with DS: 20 % of high-risk patients (2/10) and 34.5 % of low-risk patients (10/29). There was no statistically significant association of leukocytosis more than 10 × 109 /L at onset, microgranular morphology of blast cells, bcr3-variant PML::RARα, FLT3-ITD mutation with DS. In multivariate analysis, the probability of DS was associated with BMI ≥30 kg/m2 and mean fluorescence intensity of CD38 antigen by blast cells, regardless of risk group. based on the results of the ROC-analysis, the threshold value of mean CD38 fluorescence intensity was set at 25,000 cu, if exceeded, DS is highly likely to develop.

Conclusion. the high incidence of DS among low-risk patients is probably due to the lack of prophylactic glucocorticosteroids administration for the development of leukocytosis during ATRA and ATO therapy. BMI ≥30 kg/m2 and mean CD38 fluorescence intensity more than 25,000 cu were identified as statistically significant DS risk factors.
Objectification of the method for glomerular filtration rate assessing in patients with diffuse large B-cell lymphoma during induction immunochemotherapy
Authors: Nozdricheva A. S. , Lysenko I. B. , Guskova N. K. , Nikolaeva N. V. , Gaysultanova Ya. S. , Dimitriadi S. N. , Ishonina O. G.
Published: April 2024

Aim. To study the glomerular filtration rate (GFR) dynamics calculated by creatinine and cystatin C during induction immunochemotherapy in patients with newly diagnosed diffuse large B-cell lymphoma in order to objectify the method for estimation.

Materials and methods. The open longitudinal study included 39 patients with newly diagnosed diffuse large B-cell lymphoma who received specialized treatment at the Oncohematology Department of National Medical Research Centre for Oncology (Rostov-on-Don) in 2021. Patients received induction immunochemotherapy according to the R-CHOP regimen (rituximab, doxorubicin, cyclophosphamide, vincristine, and prednisolone) in combination with accompanying therapy (allopurinol 300 mg/day). Blood sampling was carried out at 0, 24, 48, 72, 120 hours and on 21st day of the 1st therapy cycle. Patients were divided into 2 groups depending on the GFR level, calculated by creatinine, before treatment: group A – 27 (69 %) patients with GFR >90 ml/min/1.73 m2, group B – 12 (31 %) patients with GFR <90 ml/min/1.73 m2.

Results. During the immunochemotherapy in patients with initially reduced GFR, a further decrease was observed with the restoration of the initial level by day 21 of therapy. When calculating GFR by cystatin C, in contrast to the calculation by creatinine, it revealed the dependence of GFR level on pathological process stage: GFR in group A patients with stages I–II is 20.4 % lower than in patients with stages III–IV, in group B – by 30.5 %. The use of the fisher test at GFR thresholds of 90 and 60 ml/min/1.73 m2 revealed a greater advantage in establishing absolute GFR levels, especially in the range of 60 to 90 ml/min/1.73 m2.

Conclusion. The data obtained confirm that the determination of GFR by cystatin C in patients with diffuse large B-cell lymphoma is a more sensitive method that objectively reflects the functional state of the kidneys, especially when values are within the “gray area” – from 90 to 60 ml/min/1.73 m2.
SIMULTANEOUS CORONARY BYPASS AND ESOPHAGECTOMY IN PATIENTS WITH ESOPHAGEAL TUMOR
Authors: Komarov Roman N. , Tkachev Maxim I. , Rogova Tatyana G. , Baluczkaya Anastasia V. , Karmanova Yulia I. , Gabdrafikova Kamila I. , Kuz`min Sergey O. , Hovanskij Dmitry V.
Published: April 2024

Highlights

The article presents data on international practices regarding simultaneous coronary bypass surgery and esophagectomy. There are very few works devoted to this topic in the literature, whereas such patients can show up unexpectedly but cannot safely undergo multiple procedures. Studying the international practices will allow specialists to make the right decision in each individual case.

 

Abstract

Coronary heart disease (CHD) remains the leading cause of death from cardiovascular disease, accounting for nearly 50% of deaths. However, recent data show a significant change in the composition of patients with CHD: patients with a concomitant diagnosis of esophageal cancer, a disease for which a new generation of novel immune and targeted therapies has altered and significantly increased life expectancy, predominate. However, the treatment algorithm for such patients is not fully known. If myocardial revascularization is performed first, radical surgery regarding the tumor will be delayed. If first to perform surgical intervention on the esophagus, then the number of complications increases, which according to some authors is 30–40 times higher than in patients without cardiac disease. One-stage intervention and simultaneous surgery allows to avoid such problems. This article summarizes the world experience of such interventions.
THORACOSCOPIC LEFT ATRIAL APPENDAGE AMPUTATION CAN BE AN ALTERNATIVE TO ENDOVASCULAR OCCLUSION
Authors: Khalimov Zakir Z. , Mamchur Sergey E. , Mamchur Irina N. , Bokhan Nikita S. , Kozyrin Kirill A. , Ivanova Anna V.
Published: April 2024

Highlights

We present a clinical case of a patient with atrial fibrillation and a high bleeding risk according to HAS-BLED bleeding risk score who underwent thoracoscopic left atrial appendage (LAA) occluder implantation due to lack of endovascular access. Thoracoscopic amputation of LAA also can be performed in cases where an endovascular access cannot be achieved due to occlusion of the inferior vena cava or thrombosis of LAA. However, it is necessary to monitor the condition of the stump when conducting thoracoscopic amputation of LAA, and stop anticoagulation therapy only in case of non-embologenic stump.

 

Abstract

Atrial fibrillation is the most common rhythm disorder that can be conservatively treated using antiarrhythmic therapy, and anticoagulants can be used to prevent thromboembolic complications. However, prolonged use of anticoagulants is complicated by the risk of bleeding. Left atrial appendage (LAA) occluder implantation is an alternative way of preventing of thromboembolic and hemorrhagic complications. The article presents a clinical case of thoracoscopic LAA amputation after unsuccessful endovascular LAA occluder implantation in the patient with the inferior vena cava occlusion.
ASSOCIATIONS BETWEEN QUALITY OF LIFE WITH PSYCHOLOGICAL STATE, DISORDERS OF THE CARDIOVASCULAR SYSTEM AND PECULIARITIES OF INFECTIOUS PNEUMONIA 3 MONTHS AFTER COVID-19
Authors: Gus'kova Olga A. , Yaroslavskaya Elena I. , Prilenskij Boris Y. , Petelina Tatyana I.
Published: April 2024

Highlights

The severity of pneumonia and the presence of cardiovascular diseases have a negative impact on the physical aspect of quality of life. Women`s quality of life indicators tend to be worse than men`s. Regular work activity is a factor that improves the physical aspect of the quality of life.

 

Abstract

Aim. To analyze the relationship between the quality of life (QoL) and the psychological and somatic state of patients 3 months after Coronavirus disease 2019 (COVID-19) pneumonia.

Methods. The study is a part of the “Prospective Follow-up Study of Patients after COVID-19 Pneumonia” that involves data on 351 patients (age 53±11) 3 months after hospitalization. Women accounted for 51% of all patients. Cardiologist and clinical psychologist examined cardiovascular and psychological health of patients. We assessed QoL by using the SF-36 survey, and psychometric properties by using the GAD-7, PHQ-9 and PSS-10. We estimated the severity of the COVID-19 using the discharge summaries information.

Results. The prevalence of stress symptoms (27,4% vs 5,1%, p = 0,030) and depression (18,00 [13,00–25,00] vs 20,00 [15,00–24,00], p = 0,032) were higher in patients with cardiovascular disorders. Moreover, QoL was lower in this group of patients in all subscales except for “Mental health” and “Mental health aspect”. Women were more 3 times more likely to experience psychological and emotional disorders compared to men (29,1% vs 16,2%, ОR = 2,615 95%; 95% CI 1,695–4,035; p<0,001). At the same time, all QoL indicators in women were significantly lower. For example, the average score on subscales “Mental health aspect” was equal to 47,17 [41,33–51,35] in women and 50,16 [46,65–52,35] in men (p<0,001), and the average score on subscale “Physical health aspect” was equal to 66,32 [60,71–72,62] in women and 63,82 [56,04–70,93] in men (p = 0,003). The QoL scores on physical health subscales were higher in patients with mild and moderate lung lesions. Scores on the “Social functioning” subscale were higher in unmarried patients (87,00 [62,50–100,00] in married patients and 100,00 [75,10–100,00] in unmarried patients; p = 0,017), and scores on the subscale “Physical health aspect” were lower in unemployed patients (48,73 [44,43–52,31] in employed patients and 44,84 [41,32–49,73] in unemployed patients; p<0,001).

Conclusion. Тhe presence of cardiovascular disorders and severity of the COVID-19 pneumonia affected the physical aspect of QoL. QoL in women is worse compared with men. Regular work activity improves the physical aspect of QoL. There is a decrease in social activity in married patients after COVID-19 compared to unmarried patients.
VARIOUS ASPECTS OF PERCUTANEOUS CORONARY INTERVENTIONS AS PREDICTORS OF DEATH IN MYOCARDIAL INFARCTION AND CORONARY MICROVASCULAR OBSTRUCTION (NO-REFLOW)
Authors: Frolov Alexey A. , Pochinka Ilya G. , Frolov Igor A. , Kuzmichev Kirill V. , Ulanova Nina D. , Mukhin Alexey S. , Sharabrin Evgeny G. , Sinyutin Vadim N.
Published: April 2024

Highlights

The review analyzed the role of various preoperative indicators as predictors of long-term mortality in patients with myocardial infarction and coronary microvascular obstruction (no-reflow) that developed during percutaneous coronary intervention. As a result of a multifactorial analysis, taking into account the confounders available for evaluation, we have found that predilation serves as an independent predictor of death within two years.

 

Abstract

Aim. To evaluate the role of various aspects of percutaneous coronary interventions (PCI) as predictors of long-term death in myocardial infarction (MI) and coronary microvascular obstruction (CMVO, no-reflow).

Methods. The unmatched case-control study included 232 patients with type 1 MI and CMVO developed during PCI. CMVO criteria were as follows: TIMI flow grade <3, Myocardial blush grade <2, ST segment resolution after PCI <70%. The “cases” group consisted of 54 (23.3%) patients who died within the next two years, the “controls” group consisted of 178 (76.7%) patients who survived. The analysis included the following indicators: predilation, high-pressure balloon post-dilation, vacuum assisted manual thromboaspiration, intra-aortic balloon pump, intracoronary administration of isosorbide dinitrate and verapamil hydrochloride, glycoprotein IIb/IIIa inhibitors and “potent” p2y12 inhibitors usage, bare-metal stents, stent implantation with exceeded rated burst pressure, 3 or more stents usage, PCI on more than one artery, the ratio of contrast agent volume to glomerular filtration rate (GFR) >3.0. A univariate comparative analysis of the groups regarding PCI aspects and potential confounders was performed (Mann-Whitney, Fisher). To control the confounders, a multivariate analysis was carried out (logistic regression).

Results. Differences were obtained for the following indicators: “predilation” – in 51 (94%) patients in the “cases” group and in 139 (78%) in the “control” group, p-value = 0.005; “intra-aortic balloon pump” – in 9 (17%) and 7 (4%) patients respectively, p-value = 0.003; “the ratio of contrast agent volume to GFR >3.0” – in 26 (48%) and 48 (27%) patients, p-value = 0.005. Multivariate analysis revealed that only predilation was an independent predictor of death within two years – odds ratio 7.38 (95% confidence interval 1.70–49.04, p-value = 0.005).

Conclusion. Predilation of the infarct-related coronary artery is an independent predictor of death within two years in MI patients who develop CMVO during PCI.
FEATURES OF THE COURSE OF STABLE CORONARY ARTERY DISEASE WITH BORDERLINE CORONARY ARTERY STENOSES DEPENDING ON THE EPICARDIAL FAT THICKNESS
Authors: Yakhontov Davyd A. , Ostanina Yuliya O. , Lukinov Vitaliy L.
Published: April 2024

Highlights

Patients with stable coronary artery disease, borderline coronary artery stenoses (BCAS) and increased thickness of epicardial adipose tissue (EAT) are characterized by development of cardiovascular diseases (CVD) at a younger age and higher levels of proinflammatory markers compared to patients without increased thickness of EAT (in case of comparable obesity class, diabetes type, angina grade and apolipoprotein B levels).

 

Aim. To study the features of the course of stable coronary artery disease with borderline coronary artery stenoses (BCAS) depending on the epicardial fat thickness.

Methods. 201 patients with stable angina of 1–3 grade with BCAS (50–70%) were included in the study. The 1st group consisted of 57 (28,4%) patients with high EAT levels, the 2nd group consisted of 144 (71,6%) patients without normal EAT levels. The results of biochemical blood tests and systemic inflammation markers (high-sensitivity C-reactive protein, IL 1, 6, 8, 10, TNF-a, cystatin С, MMP-9, NTproBNP) concentration were evaluated. The epicardial fat thickness was determined by echocardiography. All patients underwent coronary angiography.

Results. The general EAT thickness was at 6.00 [5.00; 6.50] mm in the 1st group and at 3.00 [2.30; 4.00] mm in the 2nd group (p<0.001). Hypertension (p<0.001), CAD (p<0.001), and myocardial infarction in particular (p = 0.003) were detected at a younger age in the 1st group. There were no significant differences in terms of prevalence of obesity, diabetes mellitus, angina grade and type of prescribed medication. Patients in the 1st group showed higher levels of NTproBNP (p = 0.002) and hsCRP (p = 0.027). Moreover, patients in the 1st group had a higher Apo-A1 levels (p = 0.023) and a lower Apo B/Apo-A1 ratio (p = 0.019). Proinflammatory cytokine levels were comparable, however anti-inflammatory cytokine IL-10 levels (р =0.005) and the frequency of elevated IL-10 levels (p<0.001) were lower in the 1st group. Higher cystatin C levels and the frequency of elevated cystatin C levels (p<0.001) were observed more often in patients with high EAT levels.

Conclusion. Patients with stable CAD, borderline coronary artery stenoses and high EAT levels can be characterized by cardiovascular diseases detected at a younger age and higher levels of proinflammatory markers compared with patients with normal EAT levels in case of comparable obesity class, diabetes mellitus, angina grade, and Apo B levels.
OMEGA-3 POLYUNSATURATED FATTY ACIDS ADMINISTRATION IN PREVENTION OF POSTOPERATIVE ATRIAL FIBRILLATION DURING CORONARY ARTERY BYPASS GRAFT SURGERY
Authors: Rubanenko Olesya A. , Rubanenko Anatoly O.
Published: April 2024

Highlights

The appointment of omega-3 polyunsaturated fatty acids for the prevention of postoperative atrial fibrillation during coronary artery bypass grafting in patients with coronary artery disease requires a thorough analysis of the characteristics of a patient and an assessment of clinical and echocardiographic parameters, factors influencing surgical performance, inflammation, and oxidative stress. Moreover, the dosage of the drug impacts the risk of atrial fibrillation. Omega-3 polyunsaturated fatty acids in short-term therapy reduce the frequency of new episodes of this arrhythmia after the intervention, however, this effect is offset by the combined use of statins.

 

Abstract

The article presents the available literature data on the effectiveness of the use of omega-3 polyunsaturated fatty acids in the prevention of postoperative atrial fibrillation in patients with coronary artery disease during coronary artery bypass grafting. Possible mechanisms of the drug’s antiarrhythmic action are highlighted, including its anti-inflammatory and antioxidant effects. The reasons for the decrease in the activity of omega-3 polyunsaturated fatty acids in combination with HMG-CoA reductase inhibitors are discussed.
MECHANISMS OF VASOPROTECTION AND CARDIOPROTECTION IN HYPOXYTHERAPY OF ARTERIAL HYPERTENSION AND CORONARY ARTERY DISEASE
Authors: Ignatenko Grigory A.
Published: April 2024

Highlights

The article presents a modern understanding of the main links of the functional circulatory regulation system and its response to exogenous hypoxia, as well as describes the data available on molecular mechanisms of vasoprotection and cardioprotection involved in response to hypoxytherapy in patients with arterial hypertension and coronary artery disease.

 

 

Abstract

Hypoxic conditioning has taken an important place in the range of non-drug methods for stimulating the adaptive potential of a healthy person and patients with cardiovascular diseases. There are many approaches to the use of various methods of exogenous hypoxia, however, there is no consensus on the essence of sanogenetic mechanisms that provide a multi-level functional system for maintaining oxygen supply and blood circulation. Adaptive reactions of afferent, central and efferent links of the functional circulatory regulation system in hypoxia in a healthy person are described. The results of studies of physiological systemic reactions realized by respiratory and vegetative plasticity using interval (intermittent) hypoxia are presented. In patients with arterial hypertension and coronary artery disease, the facts of chemoreflective stimulation and modulation of lung ventilation, autonomic nervous system status, especially sympathetic activity of peripheral blood vessels and vagal activation of cardiac activity have been established. The main vaso- and cardioprotective properties of interval hypoxytherapy are based on the leveling of oxidative stress, inflammation, activation of angiogenesis, and a shift in the secretory activity of the endothelium towards vasodilating, antiproliferative, and antithrombotic mediators. We present the evidence for the role of shear stress and the state of glycocalyx, angiopoietin and adrenomedulline in the regulation of vascular tone, as well as cytoprotective properties of cytoplasmic and mitochondrial metabolic factors in the formation of ischemic tolerance of cardiomyocytes. The issues of the relationship between systemic (neuronal and humoral) reactions and structural and functional responses of target cells in providing adaptive/compensatory mechanisms for regulating blood circulation under the influence of exogenous hypoxia require further research and evaluation.
Current treatment options for dry eye syndrome in children
Authors: Kovaleva Ludmila A. , Kuznetsova Tatjana V. , Baisangurova Albina A. , Zaitseva Alina A.
Published: April 2024

AIM: To analyze personalized therapy for dry eye syndrome (DES) in children, depending on the etiopathogenesis and severity of the disease.
MATERIAL AND METHODS: In total, 187 children aged 3–17 years with DES were treated. Ophthalmic medicines registered in Russia from the group of local rehydrants replacing tear fluid and stabilizing tear film were used, and a comparative analysis of their effectiveness was carried out.
RESULTS: An individual approach was implemented, and personalized therapy focused on the individual tolerability and effectiveness of medicines was developed. The principle of DES therapy is to replenish the lacrimal fluid deficiency and stabilize all layers of the tear film, considering the etiopathogenesis and severity of clinical symptoms. At this stage, the lipid layer of the tear film was normalized with the help of hypotonic oil-based preparations that can stabilize the lipid layer. All rehydrants of low, medium, and high viscosities contributed to the restoration of the water layer of the joint venture. The deficiency of the mucin layer of the tear film was compensated by hyaluronic acid and carboxymethylcellulose, which are part of the preparations that replace the tear film. The severity of DES associated with the intensity of the clinical changes in the conjunctiva and cornea was considered when designing the DES treatment regimen in children. In cases of mild and extremely severe severity, tear-replacement medicines of low viscosity showed the greatest therapeutic effectiveness. High-viscosity preparations and gels have been proven to be effective in moderate to severe DES. During the desquamation of the conjunctival and corneal epithelium, medicines with regenerating pharmacological action related to tissue repair simulators were included in the treatment regimen. Reparants included in the combined composition of tear-replacement medicines or monopreparations were used.
CONCLUSION: The effectiveness of the DES treatment algorithm in children with various nosologies and features of etiopathogenesis was analyzed considering disease severity. The proposed schemes of personalized tear-replacement and reparative therapy make it possible to prevent a chronic disease course and occurrence of complications and preserve or restore visual acuity.