bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring Lobbying Practices in Israel’s Nonprofit Advocacy Organizations: An Application of the Libby Lobbying Model
Authors: Libby Patricia , Deitrick Laura , Mano Rita
Published: October 2017

Nonprofit and voluntary associations around the world are the primary vehicle for representing the voices of citizens in the policy-making process. As scholars who are committed to advancing the role of civil society and the citizen, it is incumbent upon us to provide theoretical and practical frameworks that can assist nonprofits with this important work. In developed nations, the similarity between societal values and structures in democratic countries makes it possible to assess and advance best practices for policy advocacy regardless of the origin of those advocacy models. This research introduces a recently developed conceptual framework originally deployed to diagnose nonprofit organizations in the U.S. engaged in legislative advocacy. Applied to 12 Israeli nonprofit organizations involved in legislative advocacy and seeking to advance change through the legislative process, this paper assesses and expands the proposed model confirming that most facets of the U.S. framework were commonly used by Israeli nonprofits. There is also evidence that culturally embedded norms are the main source for deviations from the model applied in the U.S.
A Lookahead Behavior Model for Multi-Agent Hybrid Simulation
Authors: Yang Mei , Peng Yong , Ju Ru-Sheng , Xu Xiao , Yin Quan-Jun , Huang Ke-Di
Published: October 2017

In the military field, multi-agent simulation (MAS) plays an important role in studying wars statistically. For a military simulation system, which involves large-scale entities and generates a very large number of interactions during the runtime, the issue of how to improve the running efficiency is of great concern for researchers. Current solutions mainly use hybrid simulation to gain fewer updates and synchronizations, where some important continuous models are maintained implicitly to keep the system dynamics, and partial resynchronization (PR) is chosen as the preferable state update mechanism. However, problems, such as resynchronization interval selection and cyclic dependency, remain unsolved in PR, which easily lead to low update efficiency and infinite looping of the state update process. To address these problems, this paper proposes a lookahead behavior model (LBM) to implement a PR-based hybrid simulation. In LBM, a minimal safe time window is used to predict the interactions between implicit models, upon which the resynchronization interval can be efficiently determined. Moreover, the LBM gives an estimated state value in the lookahead process so as to break the state-dependent cycle. The simulation results show that, compared with traditional mechanisms, LBM requires fewer updates and synchronizations.
A Transparent Decision Support Tool in Screening for Laryngeal Disorders Using Voice and Query Data
Authors: Minelga Jonas , Verikas Antanas , Vaiciukynas Evaldas , Gelzinis Adas , Bacauskiene Marija
Published: October 2017

The aim of this study is a transparent tool for analysis of voice (sustained phonation /a/) and query data capable of providing support in screening for laryngeal disorders. In this work, screening is concerned with identification of potentially pathological cases by classifying subject’s data into ’healthy’ and ’pathological’ classes as well as visual exploration of data and automatic decisions. A set of association rules and a decision tree, techniques lending themselves for exploration, were generated for pathology detection. Data pairwise similarities, estimated in a novel way, were mapped onto a 2D metric space for visual inspection and analysis. Accurate identification of pathological cases was observed on unseen subjects using the most discriminative query parameter and six audio parameters routinely used by otolaryngologists in a clinical practice: equal error rate (EER) of 11.1% was achieved using association rules and 10.2% using the decision tree. The EER was further reduced to 9.5% by combining results from these two classifiers. The developed solution can be a useful tool for Otolaryngology departments in diagnostics, education and exploratory tasks.
Muscle–Bone Crosstalk: Emerging Opportunities for Novel Therapeutic Approaches to Treat Musculoskeletal Pathologies
Authors: Maurel Delphine , Jähn Katharina , Lara-Castillo Nuria
Published: October 2017

Osteoporosis and sarcopenia are age-related musculoskeletal pathologies that often develop in parallel. Osteoporosis is characterized by a reduced bone mass and an increased fracture risk. Sarcopenia describes muscle wasting with an increasing risk of injuries due to falls. The medical treatment of both diseases costs billions in health care per year. With the impact on public health and economy, and considering the increasing life expectancy of populations, more efficient treatment regimens are sought. The biomechanical interaction between both tissues with muscle acting on bone is well established. Recently, both tissues were also determined as secretory endocrine organs affecting the function of one another. New exciting discoveries on this front are made each year, with novel signaling molecules being discovered and potential controversies being described. While this review does not claim completeness, it will summarize the current knowledge on both the biomechanical and the biochemical link between muscle and bone. The review will highlight the known secreted molecules by both tissues affecting the other and finish with an outlook on novel therapeutics that could emerge from these discoveries.
Assessing Social and Territorial Vulnerability on Real Estate Submarkets
Authors: Barreca Alice , Curto Rocco , Rolando Diana
Published: October 2017

The concept of social vulnerability is widely studied in literature in order to identify particularly socially fragile sectors of the population. For this purpose, several studies have adopted indexes to measure the economic and social conditions of the population. The aim of this paper is to investigate the link between social and territorial vulnerability and the real estate market, by means of an exploratory analysis related to the possibility that spatial analyses can help to identify spatial latent components and variables in the process of price determination. A three phase approach is proposed, using the geographical segmentation of Turin and its related submarkets as a case study. After the identification and analysis of a set of three social and territorial vulnerability indicators, a traditional hedonic approach was applied to measure their influence on property listing prices. Subsequently, spatial analyses were investigated to focus on the spatial components of the indicators and property prices; their spatial autocorrelation was measured and the presence of spatial dependence was taken into account by applying a spatial regression. Results demonstrated that two indicators were spatially correlated with property prices and had a significant and negative influence on them. The proposed approach may help not only to identify the most vulnerable urban areas characterized by the lowest property prices, but also to support the future modification to the actual geographical segmentation of Turin.
Application of Sodium Selenite in the Prevention and Treatment of Cancers
Authors: Kieliszek Marek , Lipinski Boguslaw , Błażejak Stanisław
Published: October 2017

Selenium is an essential trace element that occurs in nature, in both inorganic and organic forms. This element participates in numerous biochemical processes, including antioxidant potential, but the mechanism of its anti-cancer action is still not well known. It should be noted that the anti-cancer properties of selenium depends on its chemical form, therapeutic doses, and the tumor type. Higher nutritional doses of selenium can stimulate human immune system. There are several hypotheses concerning the anticancer activity of selenium, including oxidation of sulfhydryl groups in proteins causing their conformational alterations. Conformational changes in proteins have the ability to weaken the activity of enzymes involved in the metabolism of cancer cells. In case of human fibrinogen sodium selenite, but not selenate, it inhibits protein disulfide exchange reactions, thus preventing formation of a hydrophobic polymer termed parafibrin, circulatory accumulation, of which is associated with numerous degenerative diseases. Parafibrin can specifically form a protein coat around tumor cells that is completely resistant to degradation induced with lymphocyte protease. In this way, cancer cells become protected against destruction by the organism’s immune system. Other possible mechanisms of anticancer action of selenium are being still investigated.
Solitary Intra-Osseous Myofibroma of the Jaw: A Case Report and Review of Literature
Authors: Dhupar Anita , Carvalho Karla , Sawant Poonam , Spadigam Anita , Syed Shaheen
Published: October 2017

Myofibroma is a rare benign spindle cell neoplasm in children that usually affects both soft tissue and bone in the head and neck region. Approximately one third of these cases are seen within jaw bones as solitary lesions. Solitary intra-osseous myofibroma of the jaw bone shares its clinical, radiographic and histological features with other spindle cell tumors. The rarity of this lesion can make diagnosis difficult for clinicians and pathologists. We report a case of a solitary intra-osseous myofibroma in the mandible of a nine-year-old child.
Pediatric Perioperative Pulmonary Arterial Hypertension: A Case-Based Primer
Authors: Shah Shilpa , Szmuszkovicz Jacqueline
Published: October 2017

The perioperative period is an extremely tenuous time for the pediatric patient with pulmonary arterial hypertension. This article will discuss a multidisciplinary approach to preoperative planning, the importance of early identification of pulmonary hypertensive crises, and practical strategies for postoperative management for this unique group of children.
3D NAND Flash Based on Planar Cells
Authors: Silvagni Andrea
Published: October 2017

In this article, the transition from 2D NAND to 3D NAND is first addressed, and the various 3D NAND architectures are compared. The article carries out a comparison of 3D NAND architectures that are based on a “punch-and-plug” process—with gate-all-around (GAA) cell devices—against architectures that are based on planar cell devices. The differences and similarities between the two classes of architectures are highlighted. The differences between architectures using floating-gate (FG) and charge-trap (CT) devices are also considered. Although the current production of 3D NAND is based on GAA cell devices, it is suggested that architectures with planar cell devices could also be viable for mass production.
Non-Invasive Assessment of Skin Barrier Properties: Investigating Emerging Tools for In Vitro and In Vivo Applications
Authors: Duffy Emer , Guzman Keana De , Wallace Robert , Murphy Ronan , Morrin Aoife
Published: October 2017

There is increasing interest in the development of non-invasive tools for studying the properties of skin, due to the potential for non-destructive sampling, reduced ethical concerns and the potential comparability of results in vivo and in vitro. The present research focuses on the use of a range of non-invasive approaches for studying skin and skin barrier properties in human skin and human skin equivalents (HSE). Analytical methods used include pH measurements, electrical sensing of the epidermis and detection of volatile metabolic skin products. Standard probe based measurements of pH and the tissue dielectric constant (TDC) are used. Two other more novel approaches that utilise wearable platforms are also demonstrated here that can assess the electrical properties of skin and to profile skin volatile species. The potential utility of these wearable tools that permit repeatability of testing and comparability of results is considered through application of our recently reported impedance-based tattoo sensors and volatile samplers on both human participants and HSEs. The HSE exhibited a higher pH (6.5) and TDC (56) than human skin (pH 4.9–5.6, TDC 29–36), and the tattoo sensor revealed a lower impedance signal for HSEs, suggesting the model could maintain homeostasis, but in a different manner to human skin, which demonstrated a more highly resistive barrier. Characterisation of volatiles showed a variety of compound classes emanating from skin, with 16 and 27 compounds identified in HSEs and participants respectively. The continuing development of these tools offers potential for improved quality and relevance of data, and potential for detection of changes that are undetectable in traditional palpable and visual assessments, permitting early detection of irritant reactions.
Relationship between Bone Health Biomarkers and Cardiovascular Risk in a General Adult Population
Authors: Vassalle Cristina , Sabatino Laura , Cecco Pietro Di , Maltinti Maristella , Ndreu Rudina , Maffei Silvia , Pingitore Alessandro
Published: October 2017

Purpose/Introduction: Osteoporosis (OP) and cardiovascular (CV) disease emerge as closely related conditions, showing common risk factors and/or pathophysiological mechanisms. The aim of this study was to evaluate the association between bone health markers (BHM) and individual CV risk factors and overall CV risk (FRAMINGHAM-FRS, and PROCAM scores) in a general adult population. Methods: In 103 subjects (21 males; age: 56 ± 12 years), vitamin D (25(OH)D), osteocalcin (OC), bone alkaline phospatase (BALP), procollagen I aminoterminal propeptide (P1NP), CTx-telopeptide, as well clinical history and life style were evaluated. Results: Aging (p < 0.001) and glycemia (p < 0.05) emerged as independent 25(OH)D predictors. Aging (p < 0.001), male sex (p < 0.05), and obesity (p < 0.05) represented independent OC determinants. Aging (p < 0.05) was the only independent BALP determinant. After multivariate adjustment, low 25(OH)D (<20 ng/mL) (Odds ratio OR (95% confidence intervals CI)) (5 (1.4–18) p < 0.05) and elevated OC (>75th percentile-16.6 ng/mL) (6.7 (1.9–23.8) p < 0.01) were found to be significant FRS predictors, while subjects with elevated OC and/or BALP (>75th percentile-9.8 μg/L) showed a higher CV risk as estimated by PROCAM (3.6 (1.2–10.7) p < 0.05). CTx and P1NP did not significantly correlate with CV risk factors or scores. Conclusion: As we go further into bone and CV physiology, it is evident that a close relationship exists between these diseases. Further studies are needed to investigate mechanisms by which bone turnover markers are related to metabolic risk and could modulate CV risk. This knowledge may help to develop possible multiple-purpose strategies for both CV disease and OP prevention and treatment.
Entropy Analysis of Short-Term Heartbeat Interval Time Series during Regular Walking
Authors: Shi Bo , Zhang Yudong , Yuan Chaochao , Wang Shuihua , Li Peng
Published: October 2017

Entropy measures have been extensively used to assess heart rate variability (HRV), a noninvasive marker of cardiovascular autonomic regulation. It is yet to be elucidated whether those entropy measures can sensitively respond to changes of autonomic balance and whether the responses, if there are any, are consistent across different entropy measures. Sixteen healthy subjects were enrolled in this study. Each subject undertook two 5-min ECG measurements, one in a resting seated position and another while walking on a treadmill at a regular speed of 5 km/h. For each subject, the two measurements were conducted in a randomized order and a 30-min rest was required between them. HRV time series were derived and were analyzed by eight entropy measures, i.e., approximate entropy (ApEn), corrected ApEn (cApEn), sample entropy (SampEn), fuzzy entropy without removing local trend (FuzzyEn-g), fuzzy entropy with local trend removal (FuzzyEn-l), permutation entropy (PermEn), conditional entropy (CE), and distribution entropy (DistEn). Compared to resting seated position, regular walking led to significantly reduced CE and DistEn (both p ≤ 0.006; Cohen’s d = 0.9 for CE, d = 1.7 for DistEn), and increased PermEn (p < 0.0001; d = 1.9), while all these changes disappeared after performing a linear detrend or a wavelet detrend (<~0.03 Hz) on HRV. In addition, cApEn, SampEn, FuzzyEn-g, and FuzzyEn-l showed significant decreases during regular walking after linear detrending (all p < 0.006; 0.8 < d < 1), while a significantly increased ApEn (p < 0.0001; d = 1.9) and a significantly reduced cApEn (p = 0.0006; d = 0.8) were observed after wavelet detrending. To conclude, multiple entropy analyses should be performed to assess HRV in order for objective results and caution should be paid when drawing conclusions based on observations from a single measure. Besides, results from different studies will not be comparable unless it is clearly stated whether data have been detrended and the methods used for detrending have been specified.
Signal Consensus in TSP of the Same Grid in Road Network
Authors: Li Dongyuan , Li Chengshuai , Wang Zidong , Wang Deqiang , Xing Jianping , Zhang Bo
Published: October 2017

In this paper, we propose a consensus algorithm with input constraints for traffic light signals in transit signal priority (TSP). TSP ensures control strategy of traffic light signals can be adjusted and applied according to the real-time traffic status, and provides priority for buses. We give the convergence conditions of the consensus algorithms with and without input constraints in TSP respectively and analyze the convergence performance of them by using matrix theory and graph theory, and PTV-VISSIM is used to simulate the traffic accident probability of three cases at intersections. Simulation results are presented that a consensus is asymptotically reached for all weights of priority; the algorithm with input constraints is more suitable for TSP than the algorithm without input constraints, and the traffic accident rate is reduced.
Exploring Data Model Relations in OpenStreetMap
Authors: Pruvost Hippolyte , Mooney Peter
Published: October 2017

The OpenStreetMap (OSM) geographic data model has three principal object types: nodes (points), ways (polygons and polylines), and relations (logical grouping of all three object types to express real-world geographical relationships). While there has been very significant analysis of OSM over the past decade or so, very little research attention has been given to OSM relations. In this paper, we provide an exploratory overview of relations in OSM for four European cities. In this exploration, we undertake analysis of relations to assess their complexity, composition and flexibility within the OSM data model. We show that some of the patterns discovered by researchers related to OSM nodes and ways also exist in relations. We find some other interesting aspects of relations which we believe can act as a catalyst for a more sustained future research effort on relations in OSM. These aspects include: the potential influence of bulk imports of geographical data to OSM, tagging of relations, and contribution patterns of edits to OSM relations.
SARchaeology—Detecting Palaeochannels Based on High Resolution Radar Data and Their Impact of Changes in the Settlement Pattern in Cilicia (Turkey)
Authors: Rutishauser Susanne , Erasmi Stefan , Rosenbauer Ralph , Buchbach Ralf
Published: October 2017

The fertile alluvial plain of Cilicia is bordered by the Taurus and Amanus mountain ranges to the west, north and east and the Mediterranean Sea to the south. Since the Neolithic Period, Plain Cilicia was an important interface between Anatolia and the Levant. The alluvial plain is dominated by three rivers: Tarsus, Seyhan and Ceyhan. The avulsion history of the lower course of the rivers Seyhan and Ceyhan during the Holocene remains an unresolved issue. The knowledge about how former river courses have changed is essential for the identification of ancient toponyms with archaeological sites. The analysis of silted up riverbeds based on high-resolution digital elevation models (TanDEM-X) and historic satellite imagery (CORONA) in this paper provide the first indications for the reconstruction of former river channels. Further evidence is given by the evaluation of the settlement patterns from 3rd to 1st millennium BC.
Association between Obesity and Puberty Timing: A Systematic Review and Meta-Analysis
Authors: Li Wenyan , Liu Qin , Deng Xu , Chen Yiwen , Liu Shudan , Story Mary
Published: October 2017

This systematic review and meta-analysis examined the associations between obesity and puberty timing based on scientific evidence. Eight electronic databases were searched up to February 2017 for eligible studies, and two reviewers screened the articles and extracted the data independently. A total of 11 cohort studies with 4841 subjects met the inclusion criteria. Compared with the group of normal-weight girls, the obese group had more girls with menarche (RR: 1.87, 95% CI: 1.59–2.19, 2 studies). The number of girls with early puberty was significantly higher in the obese group than the normal weight group (RR: 2.44, 95% CI: 1.32–4.52, 5 studies). However, no differences were detected between girls who were obese or normal weight at age of menarche (WMD: −0.53 years, 95% CI: −1.24–0.19, 2 studies). There is no consistent result in the relationship between obesity and timing of pubertal onset in boys. Obesity may contribute to early onset of puberty in girls, while in boys, there is insufficient data. Given the limited number of cohort studies included in this meta-analysis, high-quality studies with strong markers of puberty onset, as well as standardized criteria for defining obesity are needed.
Impact of Psychosocial Environment on Young Children’s Emotional and Behavioral Difficulties
Authors: Grazuleviciene Regina , Andrusaityte Sandra , Petraviciene Inga , Balseviciene Birute
Published: October 2017

Objective: The impact of maternal psychosocial stress on young children’s mental difficulties is unclear. This study investigated the joint effects of the socioeconomic status and parent-child relationships on emotional and behavioral difficulties in preschool children. Methods: The case-control study included 1416 mothers and their 4–6 year-old children pairs, living in Kaunas city, Lithuania. The parent-child relationships were measured using the Parent-Child Dysfunctional Interaction subscale. Children’s mental health difficulties were assessed by the Strengths and Difficulties Questionnaire. We used logistic regression models to indicate the strength of the associations. Results: Lower socioeconomic status women more often than higher ones reported pathological mother-child relations. Low education level was associated with statistically significant increase adjusted odds ratios for emotional symptoms and total behavioral difficulties. With reference to the group of better-educated mothers and normal mother-child relations, low education and pathological mother-child relations statistically significantly increased the risk of total difficulties in 4–6 year-old children; the adjusted odds ratios were 2.45; 95% CI 1.51–3.99. Conclusions: Pathological mother-child relations strengthened the effect of lower education on the increased risk of emotional and behavioral difficulties in preschool-age children. Measures oriented towards health behavior and psychosocial difficulties management may decrease children’s emotional and behavioral difficulties.
Endocrine Disruptors Leading to Obesity and Related Diseases
Authors: Petrakis Demetrios , Vassilopoulou Loukia , Mamoulakis Charalampos , Psycharakis Christos , Anifantaki Aliki , Sifakis Stavros , Docea Anca , Tsiaoussis John , Makrigiannakis Antonios , Tsatsakis Aristides
Published: October 2017

The review aims to comprehensively present the impact of exposure to endocrine disruptors (EDs) in relation to the clinical manifestation of obesity and related diseases, including diabetes mellitus, metabolic syndrome, cardiovascular diseases, carcinogenesis and infertility. EDs are strong participants in the obesity epidemic scenery by interfering with cellular morphological and biochemical processes; by inducing inflammatory responses; and by presenting transcriptional and oncogenic activity. Obesity and lipotoxicity enhancement occur through reprogramming and/or remodeling of germline epigenome by exposure to EDs. Specific population groups are vulnerable to ED exposure due to current dietary and environmental conditions. Obesity, morbidity and carcinogenicity induced by ED exposure are an evolving reality. Therefore, a new collective strategic approach is deemed essential, for the reappraisal of current global conditions pertaining to energy management.
Diurnal Hypothalamic-Pituitary-Adrenal Axis Measures and Inflammatory Marker Correlates in Major Depressive Disorder
Authors: Doolin Kelly , Farrell Chloe , Tozzi Leonardo , Harkin Andrew , Frodl Thomas , O’Keane Veronica
Published: October 2017

Dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis and inflammatory systems is a consistent finding in patients with Major Depressive Disorder (MDD). Cortisol is often assessed by measurement of the cortisol awakening response (CAR) and/or diurnal cortisol levels. Some methods of cortisol measurement overestimate cortisol concentration due to detection of other glucocorticoids including the relatively inert cortisone, therefore this study aimed to assess the presence of both cortisol and cortisone, and the cortisol-cortisone catalyzing enzyme 11β-hydroxysteroiddehydrogenase type 1 (11β-HSD1), in depressed patients and controls. Because the HPA axis is known to regulate the body’s immune system, relationships between measures of cytokines and cortisol were also assessed. Saliva samples were collected from 57 MDD patients and 40 healthy controls at five post-wakening time points (0, +30, +60, +720 and +750 min). Glucocorticoid concentrations were measured by liquid chromatography mass spectrometry. Whole blood mRNA expression of several inflammatory markers was measured by quantitative polymerase chain reaction. This study replicated the common finding of elevated morning cortisol and reduced CAR reactivity in MDD and found no differences in cortisone or 11β-HSD1 mRNA measures. There was a negative association between interleukin 1-β (IL-1β) mRNA and morning cortisol reactivity within the depressed group, indicating that dysregulation of the HPA axis and immune system may be interconnected.
Production and Use of Hymenolepis diminuta Cysticercoids as Anti-Inflammatory Therapeutics
Authors: Smyth Kendra , Morton Claire , Mathew Amanda , Karuturi Sahil , Haley Cliff , Zhang Min , Holzknecht Zoie , Swanson Chelsea , Lin Shu , Parker William
Published: October 2017

Helminthic therapy has shown considerable promise as a means of alleviating some inflammatory diseases that have proven resistant to pharmaceutical intervention. However, research in the field has been limited by a lack of availability to clinician scientists of a helminth that is relatively benign, non-communicable, affordable, and effectively treats disease. Previous socio-medical studies have found that some individuals self-treating with helminths to alleviate various diseases are using the rat tapeworm (cysticercoid developmental stage of Hymenolepis diminuta; HDC). In this study, we describe the production and use of HDCs in a manner that is based on reports from individuals self-treating with helminths, individuals producing helminths for self-treatment, and physicians monitoring patients that are self-treating. The helminth may fit the criteria needed by clinical scientists for clinical trials, and the methodology is apparently feasible for any medical center to reproduce. It is hoped that future clinical trials using this organism may shed light on the potential for helminthic therapy to alleviate inflammatory diseases. Further, it is hoped that studies with HDCs may provide a stepping stone toward population-wide restoration of the biota of the human body, potentially reversing the inflammatory consequences of biota depletion that currently affect Western society.
Effect of Wafer Level Underfill on the Microbump Reliability of Ultrathin-Chip Stacking Type 3D-IC Assembly during Thermal Cycling Tests
Authors: Lee Chang-Chun
Published: October 2017

The microbump (μ-bump) reliability of 3D integrated circuit (3D-IC) packaging must be enhanced, in consideration of the multi-chip assembly, during temperature cycling tests (TCT). This research proposes vehicle fabrications, experimental implements, and a nonlinear finite element analysis to systematically investigate the assembled packaging architecture that stacks four thin chips through the wafer level underfill (WLUF) process. The assembly of μ-bump interconnects by daisy chain design shows good quality. Results of both TCT data and the simulation indicate that μ-bumps with residual SnAg solders can reach more than 1200 fatigue life cycles. Moreover, several important design factors in the present 3D-IC package influence μ-bump reliability. Analytical results show that the μ-bump’s thermo-mechanical reliability can be improved by setting proper chip thickness, along with a WLUF that has a low elastic modulus and a small coefficient of thermal expansion.
Molecular Affinity of Mabolo Extracts to an Octopamine Receptor of a Fruit Fly
Authors: Dacanay Francoise , Ladra Ma. , Junio Hiyas , Nellas Ricky
Published: October 2017

Essential oils extracted from plants are composed of volatile organic compounds that can affect insect behavior. Identifying the active components of the essential oils to their biochemical target is necessary to design novel biopesticides. In this study, essential oils extracted from Diospyros discolor (Willd.) were analyzed using gas chromatography mass spectroscopy (GC-MS) to create an untargeted metabolite profile. Subsequently, a conformational ensemble of the Drosophila melanogaster octopamine receptor in mushroom bodies (OAMB) was created from a molecular dynamics simulation to resemble a flexible receptor for docking studies. GC-MS analysis revealed the presence of several metabolites, i.e. mostly aromatic esters. Interestingly, these aromatic esters were found to exhibit relatively higher binding affinities to OAMB than the receptor’s natural agonist, octopamine. The molecular origin of this observed enhanced affinity is the π -stacking interaction between the aromatic moieties of the residues and ligands. This strategy, computational inspection in tandem with untargeted metabolomics, may provide insights in screening the essential oils as potential OAMB inhibitors.
GC-MS Profiling of Volatile Components in Different Fermentation Products of Cordyceps Sinensis Mycelia
Authors: Zhang Hongyang , Li Yahui , Mi Jianing , Zhang Min , Wang Yuerong , Jiang Zhihong , Hu Ping
Published: October 2017

The fermentation products of Cordyceps sinensis (C. sinensis) mycelia are sustainable substitutes for natural C. sinensis. However, the volatile compositions of the commercial products are still unclear. In this paper, we have developed a simultaneous distillation-extraction (SDE) and gas chromatography-mass spectrometry (GC-MS) method for the profiling of volatile components in five fermentation products. A total of 64, 39, 56, 52, and 44 components were identified in the essential oils of Jinshuibao capsule (JSBC), Bailing capsule (BLC), Zhiling capsule (ZLC), Ningxinbao capsule (NXBC), and Xinganbao capsule (XGBC), respectively. 5,6-Dihydro-6-pentyl-2H-pyran-2-one (massoia lactone) was first discovered as the dominant component in JSBC volatiles. Fatty acids including palmitic acid (C16:0) and linoleic acid (C18:2) were also found to be major volatile compositions of the fermentation products. The multivariate partial least squares-discriminant analysis (PLS-DA) showed a clear discrimination among the different commercial products as well as the counterfeits. This study may provide further chemical evidences for the quality evaluation of the fermentation products of C. sinensis mycelia.
Dermaseptin-PH: A Novel Peptide with Antimicrobial and Anticancer Activities from the Skin Secretion of the South American Orange-Legged Leaf Frog, Pithecopus (Phyllomedusa) hypochondrialis
Authors: Huang Linyuan , Chen Dong , Wang Lei , Lin Chen , Ma Chengbang , Xi Xinping , Chen Tianbao , Shaw Chris , Zhou Mei
Published: October 2017

The dermaseptin peptides, mainly derived from the skin secretions of Hylidae frogs, belong to a superfamily of antimicrobial peptides and exhibit diverse antimicrobial and anticancer activities with low cytotoxicity. Here, we reported a novel dermaseptin peptide, from the South American orange-legged leaf frogs, Pithecopus (Phyllomedusa) hypochondrialis, processing the shortest peptide length, namely Dermaseptin-PH. The complementary DNA (cDNA) encoding biosynthetic precursor of Dermaseptin-PH was initially identified by the rapid amplification of cDNA ends PCR (RACE-PCR) technique from the skin secretion. The predicted primary structure was confirmed by a combination of reverse-phase high performance liquid chromatography (RP-HPLC) and MS/MS fragmentation from the skin secretion. Chemically-synthetic Dermaseptin-PH was investigated using a range of bioactivity assessment assays to evaluate the biological activities and cytotoxicity of Dermaseptin-PH. Dermaseptin-PH inhibited the growth of Gram-negative bacteria, Gram-positive bacteria, and pathogenic yeast Candida albicans. In addition, Dermaseptin-PH showed a broad-spectrum of anticancer activities against several cancer cell lines including MCF-7, H157, U251MG, MDA-MB-435S, and PC-3. The potent antimicrobial and anticancer activities of Dermaseptin-PH make it a promising candidate in the discovery of new drugs for clinical applications, and the relatively short sequence of Dermaseptin-PH can provide new insight for the research and structural modification of new peptide drugs.
Design of Novel, Water Soluble and Highly Luminescent Europium Labels with Potential to Enhance Immunoassay Sensitivities
Authors: Sund Henri , Blomberg Kaj , Meltola Niko , Takalo Harri
Published: October 2017

To meet the continual demands of more-sensitive immunoassays, the synthesis of novel luminescent Eu(III) chelate labels having similar substituted 4-(phenylethynyl)pyridine chromophores in three different chelate structure classes are reported. Significantly enhanced luminescence intensities were obtained, evidently caused by the intra-ligand charge transfer (ILCT) mediated sensitization, but the alternative ligands triplet state process cannot be ruled out. Based on the present study, even quite small changes on the chelate structure, and, especially, on the substituents’ donor/acceptor strength on both ends of 4-(phenylethynyl)pyridine subunits have an unpredictable effect on the luminescence. The highest observed brightness was 16,400 M−1cm−1 in solution and 69,500 M−1cm−1 on dry surface, being 3.4 and 8.7 fold higher compared to the reference chelate. The new label chelates provide solutions for improved assay sensitivity up-to tenfold from the present concepts.
Diosgenin-Rich Yam Extract Enhances Cognitive Function: A Placebo-Controlled, Randomized, Double-Blind, Crossover Study of Healthy Adults
Authors: Tohda Chihiro , Yang Ximeng , Matsui Mie , Inada Yuna , Kadomoto Emika , Nakada Shotaro , Watari Hidetoshi , Shibahara Naotoshi
Published: October 2017

Diosgenin, a yam-derived compound, was found to facilitate the repair of axonal atrophy and synaptic degeneration and improve memory dysfunction in a transgenic mouse model of Alzheimer’s disease (AD). It was also found to enhance neuronal excitation and memory function even in normal mice. We hypothesized that diosgenin, either isolated or in an extract, may represent a new category of cognitive enhancers with essential activities that morphologically and functionally reinforce neuronal networks. This study aimed to investigate the effects of a diosgenin-rich yam extract on cognitive enhancement in healthy volunteers. For this placebo-controlled, randomized, double-blind, crossover study, 28 healthy volunteers (age: 20–81 years) were recruited from Toyama Prefecture, Japan, and was randomly assigned to receive either a yam extract or placebo. Preliminary functional animal experiments indicated that an oil solvent mediated the most efficient distribution of diosgenin into the blood and brain after oral administration, and was a critical factor in the cognitive benefits. Therefore, test samples (placebo and yam extract) were prepared with olive oil and formulated as soft capsules. The intake period was 12 weeks, and a 6-week washout period separated the two crossover intake periods. The Japanese version of the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) test was used for neurocognitive assessment, and the adverse effects were monitored through blood testing. Diosgenin-rich yam extract consumption for 12 weeks yielded significant increases in total RBANS score. Among the 12 individual standard cognitive subtests, diosgenin-rich yam extract use significantly improved the semantic fluency. No adverse effects were reported. The diosgenin-rich yam extract treatment appeared to safely enhance cognitive function in healthy adults.
Atomistic Molecular Dynamics Simulations of the Initial Crystallization Stage in an SWCNT-Polyetherimide Nanocomposite
Authors: Nazarychev Victor , Larin Sergey , Lyulin Alexey , Dingemans Theo , Kenny Jose , Lyulin Sergey
Published: October 2017

Crystallization of all-aromatic heterocyclic polymers typically results in an improvement of their thermo-mechanical properties. Nucleation agents may be used to promote crystallization, and it is well known that the incorporation of nanoparticles, and in particular carbon-based nanofillers, may induce or accelerate crystallization through nucleation. The present study addresses the structural properties of polyetherimide-based nanocomposites and the initial stages of polyetherimide crystallization as a result of single-walled carbon nanotube (SWCNT) incorporation. We selected two amorphous thermoplastic polyetherimides ODPA-P3 and aBPDA-P3 based on 3,3′,4,4′-oxydiphthalic dianhydride (ODPA), 2,3′,3,4′-biphenyltetracarboxylic dianhydride (aBPDA) and diamine 1,4-bis[4-(4-aminophenoxy)phenoxy]benzene (P3) and simulated the onset of crystallization in the presence of SWCNTs using atomistic molecular dynamics. For ODPA-P3, we found that the planar phthalimide and phenylene moieties show pronounced ordering near the CNT (carbon nanotube) surface, which can be regarded as the initial stage of crystallization. We will discuss two possible mechanisms for ODPA-P3 crystallization in the presence of SWCNTs: the spatial confinement caused by the CNTs and π–π interactions at the CNT-polymer matrix interface. Based on our simulation results, we propose that ODPA-P3 crystallization is most likely initiated by favorable π–π interactions between the carbon nanofiller surface and the planar ODPA-P3 phthalimide and phenylene moieties.
Examining the Moderating Role of Perceived Lack of Facilitating Conditions on Household Recycling Intention in Kano, Nigeria
Authors: Khalil Muhammad , Abdullah Sabrina , Abd Manaf Latifah , Sharaai Amir , Nabegu Aliyu
Published: October 2017

Improper handling of household solid waste causes problems that affect public health and the environment, as well as the aesthetic nature of cities. This paper aims to determine if the theory of planned behavior (TPB), and its extended version can be used to predict household recycling intention (RI), and whether perceived lack of facilitating conditions have a moderating role on households’ RI in Nigeria. Data from a sample of 393 households from Kano metropolis Nigeria were analyzed using structural equation modeling. The result reveals that the TPB Model predicts households RI in Nigeria and explains 42% of the variance in RI. Attitude is the most important predictor of RI in the TPB model (β = 0.593, p = 0.000). However, after personal norm (PN) was added into the model, the variance in RI increases to 58% and PN becomes the most important predictor of RI in the extended TPB model (β = 0.496, p = 0.000). Perceived lack of facilitating condition was found to have a significant moderating effect on households’ RI. Finally, our findings show that providing households with recycling facilities and local collections holds great promise for improving households’ intention to recycle.
A Novel Unsupervised Segmentation Quality Evaluation Method for Remote Sensing Images
Authors: Gao Han , Tang Yunwei , Jing Linhai , Li Hui , Ding Haifeng
Published: October 2017

The segmentation of a high spatial resolution remote sensing image is a critical step in geographic object-based image analysis (GEOBIA). Evaluating the performance of segmentation without ground truth data, i.e., unsupervised evaluation, is important for the comparison of segmentation algorithms and the automatic selection of optimal parameters. This unsupervised strategy currently faces several challenges in practice, such as difficulties in designing effective indicators and limitations of the spectral values in the feature representation. This study proposes a novel unsupervised evaluation method to quantitatively measure the quality of segmentation results to overcome these problems. In this method, multiple spectral and spatial features of images are first extracted simultaneously and then integrated into a feature set to improve the quality of the feature representation of ground objects. The indicators designed for spatial stratified heterogeneity and spatial autocorrelation are included to estimate the properties of the segments in this integrated feature set. These two indicators are then combined into a global assessment metric as the final quality score. The trade-offs of the combined indicators are accounted for using a strategy based on the Mahalanobis distance, which can be exhibited geometrically. The method is tested on two segmentation algorithms and three testing images. The proposed method is compared with two existing unsupervised methods and a supervised method to confirm its capabilities. Through comparison and visual analysis, the results verified the effectiveness of the proposed method and demonstrated the reliability and improvements of this method with respect to other methods.
Lithium Ion Sensors
Authors: Kamenica Megi , Kothur Raghuram , Willows Alison , Patel Bhavik , Cragg Peter
Published: October 2017

The detection and monitoring of lithium in environmental and clinical settings is becoming increasingly important. In this review, sensors incorporating conductive polymers and lithium bronzes are discussed, together with electrochemical and spectroscopic approaches. Ionophore-based methods have been employed extensively, with varying degrees of selectivity and sensitivity, and these are discussed in depth.
Towards Harmonious Coexistence in the Unlicensed Spectrum: Rational Cooperation of Operators
Authors: Bae Sunghwan , Kim Hongseok
Published: October 2017

5G New Radio (NR) operating in the unlicensed spectrum is accelerating the Fourth Industrial Revolution by supporting Internet of Things (IoT) networks or Industrial IoT deployments. Specifically, LTE-Advanced (LTE-A) is looking to achieve spectrum integration through coexistence with multi-radio access technology (RAT) systems in the same unlicensed bands with both licensed-assisted and stand-alone access. The listen-before-talk (LBT) mechanism is mainly considered to enable an LTE operator to protect other incumbent unlicensed systems. In this article, we investigate the behaviors of multiple LTE operators along with the deployment of WiFi networks in the unlicensed spectrum from both short- and long-term points of view. In countries without mandatory LBT requirements, we show that an LTE operator is susceptible to collusion with another LTE operator, thus exploiting scarce spectrum resources by deceiving other wireless networks into thinking that channels are always busy; hence, mandatory usage of LTE with LBT is highly recommended at national level to achieve harmonious coexistence in the unlicensed spectrum. We discuss several possible coexistence scenarios to resolve the operator’s dilemmaas well as to improve unlicensed spectrum efficiency among multi-RAT systems, which is viable in the near future.
Precise GNSS Positioning Using Smart Devices
Authors: Realini Eugenio , Caldera Stefano , Pertusini Lisa , Sampietro Daniele
Published: October 2017

The recent access to GNSS (Global Navigation Satellite System) phase observations on smart devices, enabled by Google through its Android operating system, opens the possibility to apply precise positioning techniques using off-the-shelf, mass-market devices. The target of this work is to evaluate whether this is feasible, and which positioning accuracy can be achieved by relative positioning of the smart device with respect to a base station. Positioning of a Google/HTC Nexus 9 tablet was performed by means of batch least-squares adjustment of L1 phase double-differenced observations, using the open source goGPS software, over baselines ranging from approximately 10 m to 8 km, with respect to both physical (geodetic or low-cost) and virtual base stations. The same positioning procedure was applied also to a co-located u-blox low-cost receiver, to compare the performance between the receiver and antenna embedded in the Nexus 9 and a standard low-cost single-frequency receiver with external patch antenna. The results demonstrate that with a smart device providing raw GNSS phase observations, like the Nexus 9, it is possible to reach decimeter-level accuracy through rapid-static surveys, without phase ambiguity resolution. It is expected that sub-centimeter accuracy could be achieved, as demonstrated for the u-blox case, if integer phase ambiguities were correctly resolved.
Development and Elucidation of a Novel Fluorescent Boron-Sensor for the Analysis of Boronic Acid-Containing Compounds
Authors: Hattori Yoshihide , Ogaki Takuya , Ishimura Miki , Ohta Yoichiro , Kirihata Mitsunori
Published: October 2017

Novel boron-containing drugs have recently been suggested as a new class of pharmaceuticals. However, the majority of current boron-detection techniques require expensive facilities and/or tedious pretreatment methods. Thus, to develop a novel and convenient detection method for boron-based pharmaceuticals, imine-type boron-chelating-ligands were previously synthesized for use in a fluorescent sensor for boronic acid containing compounds. However, the fluorescence quantum yield of the imine-type sensor was particularly low, and the sensor was easily decomposed in aqueous media. Thus, in this paper, we report the development of a novel, convenient, and stable fluorescent boron-sensor based on O- and N-chelation (i.e., 2-(pyridine-2yl)phenol), and a corresponding method for the quantitative and qualitative detection of boronic acid-containing compounds using this commercially available sensor is presented.