bluuebunny/crossref_metadata_2025 huggingface dataset:
In vitro and in vivo tropism and biodistribution of recombinant simian adenovirus type 25
Authors: Vavilova Irina V. , Ozharovskaia Tatiana A. , Zubkova Olga V. , Popova Olga , Voronina Daria V. , Goldovskaya Polina P. , Zrelkin Denis I. , Dzharullaeva Alina Sh. , Dolzhikova Inna V. , Shcheblyakov Dmitry V. , Logunov Denis Yu. , Gintsburg Alexander L.
Published: November 2024

Introduction. Recombinant adenoviruses are widely used in the development of vaccines for a variety of infectious diseases. Despite numerous clinical studies, only a few types of human (types 5 and 26) and simian (isolate Y25) adenoviruses are currently used to produce vaccine formulations. Different types of adenoviruses vary in their cellular tropism, which plays a key role in their ability to elicit an immune response.
The aim of this study was to investigate the cellular tropism of the simian adenovirus type 25 in vitro and its biodistribution in vivo in comparison with human adenoviruses types 5 and 26.
Materials and methods. The efficiency of in vitro transduction was evaluated on 15 different cell lines using recombinant adenovirus vectors expressing the enhanced green fluorescent protein (EGFP) reporter gene. In vivo biodistribution and bioluminescence imaging were evaluated in BALB/c mice after administration of recombinant adenoviral vectors encoding the luciferase reporter gene. The acute toxicity of a recombinant simian adenovirus type 25 vector was assessed in mice and rats following intramuscular or intravenous administration.
Results. Recombinant simian adenovirus effectively transduces a wide range of cells. At the same time, a higher tropism to human glioblastoma cells (GL-6) was found compared to the other two studied adenoviruses. In vivo experiments have shown that recombinant adenoviruses are mainly localized at the injection site, and transgene expression persists for 21 days. Acute toxicity studies demonstrated that simian adenovirus type 25 vector was well-tolerated, with no animal deaths or detectable toxic effects.
Conclusion. The new platform based on the recombinant simian adenovirus type 25 is not inferior to the existing and well-established delivery systems based on human adenovirus types 5 and 26. Due to its high level of gene transfer and favorable safety profile, the use of the simian adenovirus type 25 in medicine has the potential to offer many benefits for the development of vaccines against future infectious diseases.
Pediatric Dermatological Emergencies: A Systematic Review
Authors: Joshua Dr. Sophia , Elias Dr. Shaji
Published: November 2024

Pediatric dermatological emergencies encompass a wide array of conditions that demand immediate medical attention and can have significant implications for a child's health and wellbeing. Despite the critical nature of these conditions, a comprehensive overview of the current state of knowledge is essential to improve recognition and management. This systematic review aims to address this gap by synthesizing the latest evidence related to pediatric dermatological emergencies, with a specific focus on studies published between 2020 and 2022. The primary objective of this systematic review is to provide an in-depth analysis of the epidemiology, clinical presentation, diagnostic approaches, management strategies, and outcomes of pediatric dermatological emergencies. A systematic search was conducted across prominent databases, including PubMED, Embase, and Web of Science. The review includes 21 studies that met stringent inclusion criteria, which were subsequently analyzed for patient demographics, clinical characteristics, diagnostic methodologies, therapeutic interventions, and patient outcomes. The findings from the 21 selected studies consistently highlight the critical importance of early recognition and timely intervention in the management of pediatric dermatological emergencies. On average, approximately 85% of cases demonstrated improved outcomes when appropriate care was initiated promptly. The review discusses various common conditions, including severe drug reactions, cutaneous infections, inflammatory skin disorders, and traumatic dermatological emergencies, shedding light on their epidemiology and clinical features. This systematic review underscores the significance of a multidisciplinary approach, involving dermatologists, pediatricians, and critical care specialists, in addressing pediatric dermatological emergencies. It also emphasizes the evolving insights into the pathophysiology and management of these conditions and highlights the unique ...
Impacto da retinopatia diabética em crianças e adolescentes portadores de Diabetes Mellitus tipo 1
Authors: Gomes da Fonte Rafael José , Rios Sampaio Bianca , Barriquelo de Lima Lívia , Ribeiro Freixêdas Laís , Gomes Paixão Borges Viviane , Lima Barhum Lopes Maria Eduarda , Carvalho Alves Egüez Jelski Michele , Gomes Andressa Regina , Santos de Oliveira Emanuelle , Silva Gouveia Edivânia Jaqueline , Souza Silva Glauciene , Zanfrilli Pedro Henrique , Souza Moreira Dayse Camylle , Azevedo Silva Bruno , Silva Lima José Victor , Hillesheim Vinicius  Reimer
Published: November 2024

O Diabetes Mellitus tipo 1 (DM1) é uma doença autoimune que se manifesta principalmente na infância ou adolescência, caracterizando-se pelo aumento dos níveis de glicose no sangue conhecida como hiperglicemia. Essa condição afeta principalmente crianças e adolescentes, mas também pode surgir em adultos jovens. Nesse contexto, o Brasil ocupa a terceira posição entre os cinco países com maior incidência de DM 1 e apresenta um aumento anual no número de novos casos. O principal objetivo do tratamento é, principalmente, prevenir o surgimento ou a progressão das complicações crônicas decorrentes do diabetes descontrolado. Este estudo busca enfatizar a importância do controle e tratamento adequado do diabetes para mitigar os danos causados ​​pelo mau gerenciamento da condição. Foi realizada uma revisão de literatura entre 2020 e 2024, com pesquisa em plataformas online, usando descritores como "Retinopatia Diabética", "Diabetes Mellitus tipo 1" e "Complicações diabéticas em jovens", resultando na seleção de seis artigos. Quando não tratado de forma adequada, o diabetes pode evoluir para complicações graves, que incluem risco de coma, morte, disfunção sexual, alterações de humor, hipoglicemia e transtornos de ansiedade. O diagnóstico precoce e o acompanhamento são feitos por meio de exames laboratoriais. O tratamento envolve doses diárias de insulina, controle rigoroso dos níveis de glicose, prática de atividade física e uma dieta balanceada. Assim, é essencial aumentar a conscientização do público e melhorar a abordagem dos profissionais de saúde, reduzindo os riscos de comorbidades e mortalidade, além de promover a triagem precoce.
Mathematical Creativity and Giftedness in Diverse Educational Settings
Authors: Fredericks Brenton , Kainose Mhlolo Michael
Published: November 2024

As the Chair of the Local Organising Committee (LOC) for the 13th MCG Conference, it is my honour and privilege to extend a heartfelt acknowledgment to all those who have contributed to the success of this distinguished event. This conference, dedicated to advancing the frontiers of Mathematical Creativity and Giftedness, has been an exemplary testament to the power of collaboration, dedication, and intellectual exchange.
Analisis Penguatan Implementasi Program Kelas Ibu dalam Upaya Pencegahan Stunting di Kota Denpasar
Authors: Erawati Gusti Nyoman Ayu , Sumantra I Ketut , Sujana I Putu
Published: November 2024

Kelas ibu merupakan salah satu strategi intervensi sensitif dengan pendekatan keluarga untuk meningkatkan pengetahuan, ketrampilan sasaran prioritas 1000 Hari Pertama Kehidupan (HPK) pada ibu hamil dan ibu balita, dengan harapan terjadi perubahan perilaku yang bisa mencegah stunting di Kota Denpasar. Penelitian ini bertujuan mengeksplorasi faktor internal dan eksternal serta menganalisis strategi dalam implementasi penguatan program kelas ibu. Pengumpulan data dilakukan dengan menggunakan kuisioner dan selanjutnya dilakukan Fokus Group Diskusi. Analisis data menggunakan matrik IFAS, EFAS dan QSPM. Hasil penelitian didapatkan implementasi kelas ibu dalam katagori sangat baik (80,74%) terdiri dari variabel komunikasi katagori baik (71,75%), ketersediaan sumber daya katagori sangat baik (80,84%), sikap dan komitmen katagori baik (70,37%) dan katagori struktur birokrasi sangat baik (100%). Hasil analisis data faktor internal yang dominan yaitu sebagai kekuatan ketersediaan petugas terlatih, media informasi yang cukup dan terbatasnya sarana pendukung kelas ibu. Sedangkan faktor eksternal yang dominan yaitu keterkaitan rencana lintas program terhadap kelas ibu dengan memasukkan muatan lokal (yoga ibu hamil, pijat bayi) dan tantangan terbesar yaitu kurang maksimalnya keterlibatan tokoh masyarakat. Strategi prioritas penguatan kelas ibu yaitu sosialisasi kelas ibu terstruktur, sistematis dan berkelanjutan dengan mengintensifkan keterlibatan tenaga kesehatan dan lembaga swadaya masyarakat (LSM).
Pengaruh Tarikan Guna Lahan SPBU Terhadap Kinerja Jalan Talangagung
Authors: Agustin Imma Widyawati , Widartiningsih Nurizka Aprilia
Published: November 2024

In every region, but particularly in emerging ones, transportation plays a significant role in economic activity. Kepanjen District, one of Malang Regency's sub-districts, serves as the hub of the region's local activities. One of the main collector roads in Kepanjen District, Talangagung Road connects Kepanjen District with Blitar City. This portion of road is used for a number of purposes, one of which is a gas station. Determining the traction model and the impact of gas station traction in Talangagung on the road’s level of service of Talangagung is the goal of this study. Correlation analysis would be the initial method employed, followed by multiple linear analysis. Thus, a model will be created, and a simulation derived from that model will follow. The resulting gas station model is YSPBU = 1,526 + 1,159 (X2) with weekday Adjusted R2 = 0,864 and weekend Adjusted R2 = 0,832 (X4). The quantity of visits is the affecting factor. This indicates that the average impact of gas stations on Jalan Talangagung's volume is 8.56%, and it is largest during the week. On the other hand, petrol stations have an average 7.61% influence on Jalan Talangagung's volume on weekends.
Partisipasi Masyarakat Terhadap Pengelolaan Jaringan Irigasi Subak Jaka di Kabupaten Tabanan
Authors: Yasa I Made , Partama I GD Yudha , Yastika I Putu Edi
Published: November 2024

Pengelolaan jaringan irigasi mempunyai peranan penting dan strategis dalam sektor pertanian. Partisipasi masyarakat subak dalam mengelola jaringan irigasi dampaknya pada efisiensi penggunaan sumber daya air, secara tidak langsung akan membantu meningkatkan produksi pertanian. Penelitian ini dilaksanakan di Subak Jaka Kabupaten Tabanan, Provinsi Bali dari bulan Nopember 2022 sampai dengan Januari 2023. Tujuan Penelitian ini adalah: 1) menganalisis tingkat partisipasi masyarakat terhadap pengelolaan jaringan irigasi, 2) menganalisis faktor-faktor yang mempengaruhi tingkat partisipasi masyarakat subak dalam pengelolaan jaringan irigasi; dan 3) menyusun strategi penguatan partisipasi masyarakat subak dalam mengelola jaringan irigasi. Metode penelitian ini menggunakan Mix Method yaitu pertama fokus ke analisis kualitatif untuk menganalisis tingkat partisipasi, analisis kuantitatif menggunakan Analisis Regresi Linier Berganda untuk mengetahui besarnya pengaruh variabel-variabel bebas dan analisis SWOT. Hasil penelitian menyimpulkan bahwa: a) tingkat partisipasi masyarakat Subak Jaka terhadap pengelolaan jaringan irigasi dalam kategori sedang; b) faktor-faktor signifikan yang mempengaruhi tingkat partisipasi masyarakat subak dalam pengelolaan jaringan irigasi adalah faktor pendapatan dan jumlah tanggungan, dan c) strategi untuk meningkatkan partisipasi masyarakat yaitu: 1) meningkatkan penerapan awig-awig/peraturan subak secara konsisten; 2) meningkatkan sumber daya manusia masyarakat Subak dalam pengelolaan jaringan irigasi.
Strategi dan Evaluasi Program Vaksinasi Covid-19 pada Lansia di Unit Pelaksana Teknis Daerah Puskesmas Tampaksiring II Kabupaten Gianyar
Authors: Andari I Dewa Ayu Sruti , Maba I Wayan , Wiryawan I Wayan Gde , Sudipa Nyoman
Published: November 2024

Lansia merupakan sasaran proritas program vaksinasi COVID-19 karena memiliki resiko lebih tinggi untuk mengalami penyakit kritis dan dapat menjadi fatal. Case Fatality Rate sebesar 11% pada usia di atas 60 tahun. Lansia yang belum mengakses vaksinasi COVID-19 dosis pertama sebanyak 47,86%. Tujuan penelitian ini adalah untuk menganalisis faktor internal dan eksternal serta menyusun strategi peningkatan capaian vaksinasi COVID-19 pada lansia di Unit Pelaksana Teknis Daerah Puskesmas Tampaksiring II. Penelitian ini menggunakan pendekatan kualitatif kuantitatif dengan subyek penelitian dilakukan secara purposive sampling. Pengumpulan data menggunakan kuisioner yang dilanjutkan dengan Focus Grup Disscusion. Analisis data mengunakan matrik Internal Faktor Analisis Strategi dan Eksternal Faktor Analisis Strategi dengan analisa SWOT. Hasil penelitian program vaksinasi COVID-19 pada lansia di Unit Pelaksana Teknis Daerah Puskesmas Tampaksiring II termasuk katagori sangat baik. Faktor internal yang dominan sebagai kekuatan adalah tersedianya pedoman dan kebijakan vaksinasi, sumber daya manusia kesehatan, sarana prasarana, logistik, mekanisme pelayanan vaksinasi serta dukungan lintas sektor. Faktor kelemahan yang dominan adalah informasi capaian vaksinasi COVID-19 pada lansia yang terintegrasi belum tersedia ditiap dusun wilayah kerja puskesmas. Faktor eksternal sebagai ancaman terbesar adalah kondisi fisik dan komorbid pada lansia saat mengikuti vaksinasi sedangkan sebagai peluang yang dominan adalah dukungan lintas sektor dan partisipasi aparat desa dalam membantu lansia mengakses vaksinasi COVID-19. Strategi peningkatan capaian vaksinasi melalui strategi Strenght Opportunity, dengan memfasilitasi lokasi vaksinasi yang lebih mudah diakses seperti di balai dusun atau banjar dengan melibatkan aparat desa dan dukungan lintas sektor.
Strategi Pengelolaan Daerah Aliran Sungai Ayung Berkelanjutan di Gianyar, Bali
Authors: Sudipa Nyoman , Vipriyanti Nyoman Utari , Yastika Putu Edi
Published: November 2024

Daerah Aliran Sungai salah satu sumber daya penting bagi keberlangsungan hidup manusia. Fungsi Daerah Aliran Sungai adalah sebagai pemasok air dengan kuantitas dan kualitas yang baik yang dapat dipergunakan sebagai sumber irigasi, sumber air minum dan sebagai atraksi wisata. Sungai Ayung banyak dimanfaatkan untuk kegiatan pertanian, perkebunan dan aktivitas pariwisata seperti atraksi arung jeram dan atraksi pariwisata lainnya. Di sisi lain Sungai Ayung telah mengalami tekanan dari berbagai beban pencemar dari aktivitas domestik, limbah pertanian, limbah peternakan dan limbah dari aktivitas pariwisata yang ada di sepanjang Sungai Ayung, terutama masalah kesehatan lingkungan perairan menjadi hal penting agar terhindar dari pencemaran, baik secara fisik, kimia maupun mikrobiologi. Tujuan penelitian adalah merumuskan strategi pengelolaan Sungai Ayung berkelanjutan di Gianyar, Bali. Metode penelitian menggunakan pendekatan Analytical Hierarchy Process (AHP) untuk menganalisis permasalahan yang lebih kompleks dan menghasilkan alternatif-alternatif yang merupakan strategi dalam pengelolaan Sungai Ayung secara berkelanjutan. Hasil penelitian menunjukkan bahwa aspek teknologi perlu diperhatikan dalam menentukan strategi pengelolaan Sungai Ayung berkelanjutan. Aktor yang memiliki pengaruh yang kuat dalam pengelolaan Sungai Ayung adalah pemerintah. Prioritas tujuan dari pengelolaan Sungai Ayung adalah yaitu terwujudnya Sungai Ayung yang bersih dan meningkatnya kualitas air Sungai Ayung. Alternatif strategi pengelolaan Sungai Ayung berkelanjutan secara dominan dilakukan melalui melakukan sosialisasi dan pembinaan.
Strategi Implementasi Pilar 1 Program Sanitasi Total Berbasis Masyarakat (STBM) di Desa Dauh Puri Kaja Kota Denpasar
Authors: Erawati Ni Ketut Ana , Pandawani Ni Putu , Vipriyanti Nyoman Utari
Published: November 2024

Tantangan pembangunan kesehatan khususnya dalam bidang higiene dan sanitasi masih sangat besar, sehingga diperlukan intervensi secara terpadu melalui pendekatan sanitasi. Seiring dengan meningkatnya arus globalisasi berbagai sektor, membawa dampak pada permasalahan sanitasi lingkungan. Perhatian pemerintah dalam tujuan pembangunan berkelanjutan (sustainable development goals/SDGs) pada sektor lingkungan hidup adalah memastikan masyarakat mencapai akses universal air bersih dan sanitasi layak, namun masih terdapat permasalahan penduduk Buang Air Besar Sembarangan (BABS) di Kota Denpasar. Penelitian ini bertujuan untuk menganalisis kekuatan, kelemahan, peluang, tantangan dan merumuskan strategi dalam implementasi pilar 1 program Sanitasi Total Berbasis Masyarakat. Design penelitian dengan pendekatan kualitatif dan kuantitatif dengan subyek penelitian dilakukan secara purposive sampling. Pengumpulan data dilakukan dengan menggunakan kuisioner dan FGD. Hasil penelitian didapatkan implementasi pilar 1 program sanitasi total berbasis masyarakat berada dalam katagori cukup (54,57%) terdiri dari variabel komunikasi dalam katagori sangat baik (80.47%), ketersediaan sumber daya dalam katagori cukup (53.06%), sikap dan komitment dari pelaksana program dalam katagori baik (78.80%) dan struktur birokrasi dalam katagori cukup (48.39%).
Pengaruh Tarikan Guna Lahan SPBU Terhadap Kinerja Jalan Talangagung
Authors: Agustin Imma Widyawati , Widartiningsih Nurizka Aprilia
Published: November 2024

Di setiap daerah, terutama di daerah yang sedang berkembang, transportasi memainkan peran penting dalam kegiatan ekonomi. Kecamatan Kepanjen, salah satu kecamatan di Kabupaten Malang, berfungsi sebagai pusat kegiatan lokal di wilayah tersebut. Salah satu jalan kolektor utama di Kecamatan Kepanjen adalah Jalan Talangagung yang menghubungkan Kecamatan Kepanjen dengan Kota Blitar. Ruas jalan ini digunakan untuk beberapa keperluan, salah satunya adalah SPBU. Menentukan model intreaksi dan dampak interaksi SPBU di Talangagung terhadap tingkat pelayanan jalan Talangagung merupakan tujuan dari penelitian ini. Analisis korelasi akan menjadi metode awal yang digunakan, diikuti dengan analisis linier berganda. Dengan demikian, sebuah model akan dibuat, dan disimulasi yang berasal dari model tersebut. Model SPBU yang dihasilkan adalah YSPBU = 1.526 + 1.159 (X2) dengan nilai R2weekday = 0.864 dan nilai R2 weekend = 0.832 (X4). Kuantitas kunjungan merupakan faktor yang mempengaruhi. Hal ini menunjukkan bahwa dampak rata-rata SPBU terhadap volume Jalan Talangagung adalah 8,56%, dan terbesar pada weekday. Di sisi lain, SPBU memiliki pengaruh rata-rata 7,61% terhadap volume Jalan Talangagung pada weekend.
Graphical modeling of additive color mixing. Perception of objects with different color shades for different observers
Authors: Popova Teodora Petrova , Ignatov Ignat , Ignatov Alexander I. , Angushev Ivan
Published: November 2024

Aim

To research subjective perceptions in additive color mixing.

Material and methods

A survey was administered to 79 individuals, who determined the colors they perceived in two photographs. Graphical modeling is applied to determine the result of color mixing.

Results

In additive mixing of monochromatic colors, a new color is obtained. Interestingly, different individuals perceive observed images in different ways. Mix neighboring colors on the spectrum and those in different ranges. Distinction between the physical mixing of laser beams with different colors was found. The visual perception in the presence of two colors is subjective and is determined by the viewer's greater sensitivity to one of the colors. Due to additive color mixing, additional parts of objects appear golden or orange when there is a yellow color in a picture with blue tones. When the background is violet, the sensitivity of the blue cones decreases.

Conclusions

It has been confirmed that visual perception, in the presence of two colors is subjective and is determined by the viewer's greater sensitivity to one of the colors. A method is proposed that physically demonstrates the mixing of green and red colors, resulting in the colors yellow or orange.
Problems of diagnostic of the state of pulp and pulpal diagnoses in children
Authors: Kaskova Lyudmyla , Yanko Nataliia , Vashchenko Iryna , Khmil Olena
Published: November 2024

The aim: to analyze diagnostic methods of the pulp status in children and align pulpal diagnoses with international and Ukrainian classifications.
Material and methods: The authors searched for articles via Google using keywords such as «pulpitis classification», «pulp diagnostic in children», «reversible pulpitis», «irreversible pulpitis», «pulp necrosis» combined with «primary teeth», «permanent immature teeth». Also evidence of primary data was evaluated by levels of evidence statements and grades of recommendations for questions relating to diagnosis.
Results: Level of evidence of primary data about methods of pulpal state diagnosis varies from 2 to expert opinion, grade of recommendation is C-D. Sensibility tests can be used for pulp diagnosing in primary and immature permanent teeth, in conjunction with other clinical diagnostic aids. Such conditions of pulp as complete pulp necrosis, pulpless and infected root canal system, previously treated pulpitis, and previously initiated therapy could be included in the Ukrainian classification. Pulp hyperplasia only partially corresponds pulpitis chronica hypertrophica and may be associated with any form of reversible and irreversible pulpitis. In international classifications, partial pulp necrosis includes pulpitis chronica gangraenosa and pulpitis acuta purulenta from the Ukrainian variant.
Conclusions: Pulpal diagnosis in children relies on observation of clinical and periapical radiographic findings. While the Ukrainian classification covers various forms of pulpitis, it could be expanded to include pulp conditions, but only some of them completely correspond diagnoses of the Ukrainian classification. Therefore, the development of new classification of the status of the pulp in children is promising and challenging task.
Multimodal management of breast architectural distortion: an analytical literature review
Authors: Kovtun Andrii , Gurando Andrii , Telniy Vadym , Kozarenko Tetiana
Published: November 2024

The aim: The objective of this literature review was to determine the optimal diagnostic algorithm for breast cancer detection associated with architectural distortion.

Materials and methods: Pubmed, Google Scholar, Web of Science, and Scopus databases were used to search for materials on architectural distortion, associated pathologies, and radiological imaging methods.

Conclusions: Architectural distortions may represent both benign and malignant pathology, but ultrasound, magnetic resonance, and roentgenological signs may not always with confidence determine the etiology of these changes. Therefore, the correct diagnostic algorithm which uses contrast imaging methods and digital breast tomosynthesis can prevent potentially exceeding radiation exposure, and unnecessary biopsies, change surgical operation volumes, decrease patient stress from procedures, and reduce the number of resources spent. After analyzing a number of scientific sources, we recommend morphological verification of all architectural distortions that are not the result of surgery, trauma, or that accumulate contrast media during breast imaging.
Features of the macroscopic structure of the posterior inferior tibiofibular ligament based on anatomical study
Authors: Kucher Ivan V. , Liabakh Andrii P.
Published: November 2024

Aim

to study the anatomy of the posterior inferior tibiofibular ligament (PITFL) and specify the features of its morphology and linear parameters.

Material and methods

The peculiarities of morphology and linear parameters of PITFL on 10 fresh amputated lower limbs were studied. The average age of the patients was 64.7 ± 9.3 (7 males, 3 females). Macroscopic characteristics, insertion, orientation in relation to the corresponding bony and ligamentous anatomical structures were studied, and linear parameters of PITFL were measured using a caliper. Average values were calculated.

Results

PITFL was detected in all the studied samples. It represented a strong, compact anatomical structure of a trapezoidal or triangular shape. The proximal width of the PITFL averaged 20 ± 3.65 mm, while the distal width was 36.6 ± 4.62 mm. The attachment length of PITFL to the posterior part of the tibia was 28.6 ± 5.13 mm and to the fibula was 17.4 ± 3.2 mm. The ligament is closely connected to the posterior intermalleolar ligament, the inferior transverse ligament, and the tendinous sheaths of the posterior tibial and fibular muscles.

Conclusions

The obtained results of the study of linear and morphological parameters of PITFL should contribute to a better understanding of the anatomy of the posterior part of the ankle joint and improve surgical approaches to the treatment of posterior malleolus fractures and related PITFL injuries.
Features of the clinical course of arterial hypertension in patients with vitamin D deficiency
Authors: Tarasiuk Alina , Kondratiuk Vitalii , Bychkov Oleg Anatoliiovych , Ostashevska Tetiana
Published: November 2024

Aim

To determine the peculiarities of the daily profile of blood pressure in patients with arterial hypertension (AH) and vitamin D deficiency and insufficiency, to evaluate the parameters of the lipid profile and quality of life in these patients.

Material and methods

A total number of 97 patients with uncomplicated AH stage 1 and 2 were divided into 3 groups depending on the level of vitamin D in the blood serum. All patients underwent office blood pressure (BP) measurement and daily ambulatory BP monitoring for 24 hours, biochemical blood test with the determination of indicators of lipid metabolism, questionnaires for quality of life indicators. Statistical processing of the study results was carried out using “IBM SPSS Statistics 22”.

Results

"Non-dippers" are most often identified in groups 1 and 2. Their prevalence is 1.8 times higher than in group 3 (p <0.05). The frequency of "night-peackers" in group 1 is 1.7 times higher than in group 2 and 2.6 times higher than in group 3 (p < 0.05). Patients with AH and vitamin D deficiency have severe lipid metabolism disorders (p < 0.05). The correlation analysis shows a direct relationship of average strength between them and average daily systolic and diastolic BP indicators (р<0.05).

Conclusions

Patients with AH and vitamin D deficiency has an increase in cardiovascular risk, which is manifested by increase in pressure load indicators, frequency of detection of "night-peakers" and lipid metabolism disorders which is worsens of vascular accidents prognosis.
Psychological well–being in breast cancer patients: the role of social support in managing anxiety and depression
Authors: Zaviazkina Nataliia V. , Nefedova Tetiana
Published: November 2024

Aim

To investigate the role of social support in the relationship between psychological well-being, anxiety, and depression among breast cancer patients

Material and methods

A cross-sectional study included 98 women with breast cancer. Measures used were the Psychological General Well-being Index (PGWBI), the Hospital Anxiety and Depression Scale (HADS), the Multidimensional Scale of Perceived Social Support (MSPSS), and a sociodemographic questionnaire. Descriptive statistics, Cronbach's alpha, and regression analyses with moderation and mediation were conducted. T-tests estimated statistical significance in regression. Mediation effects were assessed using the quasi-Bayesian Monte Carlo method with bootstrapping.

Results

The initial linear regression analysis showed a strong negative relationship between anxiety/depression (HADS) and psychological well-being (PGWBI), with higher HADS scores were linked to lower psychological well-being. The regression analysis for social support (MSPSS) indicated a positive but small effect on psychological well-being. The moderation analysis showed no significant interaction between social support and anxiety/depression on psychological well-being. However, the mediation analysis demonstrated that social support significantly improves psychological well-being by reducing anxiety and depression, with a substantial proportion of the effect mediated.

Conclusions

The study found that while social support does not moderate the relationship between anxiety, depression and psychological well-being, it significantly enhances psychological well-being by reducing anxiety and depression.
Molecular investigate of slim and adhesion genes of "Staphylococcus saprophyticus" isolated from pregnant women with asymptomatic bacteriuria in Thi-Qar governorates
Authors: Jabur Sanaa Ghali
Published: November 2024

Aim

Aims: to investigate and compared the frequency of biofilm formation (ica A) and adhesion genes (clf A, cnaA, fnbA and fnbB) in chromosome and plasmid of ''S. saprophyticus".

Material and methods

Materials and methods: 48 isolates about "S. saprophyticus" were selective randomly from total 1019 isolated from pregnant women with asymptomatic bacteriuria where the samples were collected in AL Habboby Hospital and Al-Hussein teaching, and bent Al-Huda hospitals from the first of April 2022 until the first of September 2023 and bacteria of study were identified depend on previous method.

Results

Results: PCR appeared, higher existence percentage of study virulence factors genes was in chromosome that reached 399.9% of study bacteria, more than in its in plasmid 66.5%, frequency rate of study genes in chromosome was as: ica A and fnb A genes were complete, 100% (48/48), while cna A and Clf A genes percentage were identical and came in second grade that arrived significant rate 83.3% (40/48), the lowest results were showed 33.3% (16/48) isolates harbored fnbB gene, while they were decreased in itś plasmid as following : ica A and fnb A genes were 12.5% (6/48) and 22.9% (11/48)respectively, the cna A and Clf A genes percentage 6.2 % (3/48) and 4.1% (2/48) respectively, fnb B gene 20.8% (10/48).

Conclusions

Conclusion: Depend on above data may be ica A, fnb A, clf A, cna A genes more occurrence in comparison to fnb B genes among "S. saprophyticus" and plasmid may double the rate of same virulence study genes.
Intracranial arachnoid cyst complicated with subdural hygroma in a 10-year-old child: A case report and literature review
Authors: El Kacemi Inas , Dokponou Yao Christian Hugues , Gyamera Rosina T. , Hakkou Mehdi , Oudrhiri Mohammed Y. , Boutarbouch Mahjouba , Melhaoui Adyl , Arkha Yasser , Ouahabi Abdessamad El
Published: November 2024

Arachnoid cysts are abnormal collections of fluids within the arachnoid membrane after a doubling of the structure. These benign lesions represent only 1% of all intracranial space-occupying lesions. The affected patient can remain asymptomatic for several years, which is an incidental finding in most cases. Cysts have been reported to rupture after a minor head trauma, causing subdural hygroma or hematoma; however, spontaneous rupture complicated with subdural hygroma has been rarely reported. Herein, we report a rare case of a 10-year-old boy whose pregnancy follow-up, delivery, and parent’s medical history were unremarkable. The patient was admitted for a temporal arachnoid cyst complicated with subdural hygroma, which was determined to be caused by intracranial hypertensive syndrome. He was successfully treated through endoscopic arachnoid cyst fenestration, wherein a pathway was created for the cyst to communicate with the subarachnoid space through the basal cisterns. The choice of treatment for arachnoid cysts remains controversial. Endoscopic treatment is considered when an ipsilateral subdural hygroma is present. Intracranial hypertensive syndrome subsided immediately after surgery. No post-operative complications occurred. The child recovered uneventfully in the post-operative period and was discharged 5 days after surgery. He underwent a follow-up computed tomography 1 month later, confirming a progressive regression of the hygroma as well as arachnoid cyst.
Efficacy and safety of stereotactic radiotherapy in oligometastases
Authors: Dong Huan , Li Rong , Huang He-Qing , Chen Xing-Hua , Zhou Zi-Yan , Kang Min
Published: November 2024

Distant metastasis remains a leading cause of treatment failure in patients with cancer. While salvage treatments such as surgery, targeted therapy, and immunotherapy provide some benefits, they have inherent limitations. This study examines the efficacy and safety of stereotactic radiotherapy (SRT) in managing oligometastatic tumors. We retrospectively analyzed data from 96 patients with oligometastatic tumors who were treated with SRT at the Department of Radiotherapy of the First Affiliated Hospital of Guangxi Medical University between September 2019 and March 2023. Descriptive statistics were used to analyze patient characteristics, tumor response, and survival outcomes, whereas a single-sample ratio test was used to compare SRT efficacy across different tumor sites. Kaplan–Meier analysis was used to determine progression-free survival (PFS) and overall survival (OS). Three months post-treatment, the complete response, partial response, stable disease, and progressive disease rates were 26.32%, 50.00%, 21.05%, and 2.63% for liver oligometastases; 0.00%, 39.13%, 43.48%, and 17.39% for lung oligometastases; and 8.57%, 62.86%, 25.71%, and 2.86% for brain oligometastases, respectively. The objective response rate was 65.62%, and the disease control rate was 93.75%. The median PFS was 18 months, and the median OS was 27 months. The primary lesions were mainly located in the liver and lungs. Compared with previous studies where stereotactic ablative body radiotherapy achieved a median OS of 16 months for liver metastases and surgery yielded an OS of 7 – 24 months for non-small cell lung cancer with brain metastases, our SRT outcomes were significantly improved. Adverse events (≥grade 3), primarily hematological toxicity, occurred in only 5.21% of patients. SRT was both effective and well-tolerated among patients with oligometastatic tumors, offering a promising treatment option.
Simulated study relating dose-volume histogram metrics to three-dimensional gamma analysis results
Authors: Bonatto Larisse Neumann , Bruno Alexandre Colello , Baffa Oswaldo , Pavoni Juliana Fernandes
Published: November 2024

This study aims to investigate the relationship between the three-dimensional gamma index (3DGI) results and its clinical significance, proposing a correlation between changes in dose values in the dose-volume histogram (DVH) of the evaluated structures and 3DGI percentages of approval. In this study, we created 30 plans by including 1 mm displacement errors in the lateral, vertical, and longitudinal axes of each table, and compared them to a simulated, error-free treatment plan for prostate cancer. The plans were made in a commercial treatment planning system, using 6MV Varian 120-leaf multileaf collimators (MLC) linear accelerators and intensity modulated radiation therapy sliding window technique. We used a calculation algorithm-based MATLAB programming to evaluate the 3DGI analysis of fluence maps. The acceptance criteria were 3%/2 mm, 10% dose threshold, and 95% gamma passing rate. We compared 3DGI results with differences in structures DVH. DVH metrics versus 3DGI analysis showed a correlation between the percentage of point’s approval and planning target volume (PTV) coverage, presenting a degree of agreement of 0.85%, 0.96%, and 0.94% in the longitudinal, lateral, and vertical axes, respectively. In summary, it was possible to establish a linear relationship between the percentage of points approved in the 3DGI analysis and the PTV dose. A similar behavior was also observed in bladder and rectum DVH, but for these structures, the relationship was not the same in the three displaced axes.
Faculty Readiness on Computational Sustainability: A Literature Synthesis on the Readiness Dimensions
Authors: Tuan Rahim Tuan NurNadzirah 'Asyikin , Ismail Azniah , Ubaidullah Nor Hasbiah , Fathil Nur Saadah , Kamaruddin Kamalia Azma , Zakaria @ Mohamad Aznida Hayati , Mohd Zulkefli Nurul Akhmal
Published: November 2024

Computational sustainability has become a key topic bridging environmental science, computer science, and sustainability research. This literature review explores the readiness dimensions necessary for advancing computational sustainability projects and examines how computational tools are applied to address sustainability challenges across various domains. This study examines 33 case studies and 56 empirical research papers that demonstrate the use of computational tools to improve readiness in a variety of scenarios, including technology readiness, faculty readiness, teaching readiness, e-learning readiness, and green education. By extensively reviewing previous material, this synthesis identifies recurring themes and emerging trends in readiness assessment across many sustainability sectors, case studies, and empirical research. The study blends several views and approaches, resulting in a better understanding of how readiness aspects might aid in the application and efficacy of computational tools in sustainability research. The literature synthesis highlights the dimensions of readiness in this study, ranking technological knowledge (35%), content knowledge (25%), teaching strategies (20%), training (15%), and equipment/software (5%) based on their significance in determining how well societies are prepared to effectively adopt sustainable practices. This literature synthesis explores readiness factors in computational sustainability, highlighting recent advancements and trends. The review focused on English-language publications from 2018 to 2024, with additional research from 2010 to 2017. This comprehensive analysis of faculty readiness for computational sustainability aims to enhance its effectiveness, paving the way for broader studies that benefit researchers, faculties, students, policymakers, and society.
Assessing remote learning's feasibility: A comprehensive analysis of Philippine public-school teachers’ use of learning management systems and blended learning approaches
Authors: Prestoza Mark Jhon
Published: November 2024

This study examines the ever-changing environment of blended learning, with a special emphasis on how teachers in public schools in the Philippines use Google Classroom in the shifting educational landscape. Based on a mix of academic views and practical research, the study seeks to improve our understanding of the difficulties and possibilities related to blended learning. The conceptual framework, derived from Graham et al.'s blended teaching matrix, classifies interactions into four quadrants, offering a complete structure for comprehending blended learning situations. The study aims to identify the blended teaching approaches used by instructors, evaluate the utilization of Google Classroom features and Google Apps for Education, and ascertain the frequency and dependence of Google Classroom as a key teaching tool. Data was gathered from 123 public-school teachers at the Schools Division Office of Isabela through a systematic survey. The results demonstrate a prevalent use of Google Classroom features, highlighting its significance in facilitating the transmission of lessons, communication between students, distribution of assignments, and improvement of learning activities. An examination of the features of Google Classroom, Google Apps for Education, and Google Docs file templates reveals a wide range of use patterns among instructors, ranging from seldom to regular utilization. Teachers demonstrate favorable attitudes towards the adaptability and compatibility of Google Classroom, emphasizing its seamless connection with portable devices. Challenges arise from the sporadic Wi-Fi connections, which have a negative influence on the quality of blended learning. The research suggests implementing customized professional development program for teachers and highlights the need to include adaptive technological frameworks in curriculum design. This study provides essential knowledge on the implementation of blended learning methods by public school teachers. It gives suggestions for policymaking, curriculum design, and teacher training in response to the ever-changing educational environment.
Factors influencing the educational expectations of Chinese youth
Authors: Yifan Wu , Weiwei Pu
Published: November 2024

In China, education is an indispensable part of the growth of teenagers, and the results of education are also the achievements that teenagers should achieve at this stage. Educational expectation as a socio-psychological variable has an important impact on student's educational results. In the “Wisconsin Model”, educational expectation as an intermediary variable has been a concern by researchers Unfortunately, limited studies were found about the influencing factors of educational expectation. As a result, this study will examine the influencing factors on the educational expectations of Chinese teens using a sample of teenagers aged 13 to 15 as well as the feedback from their parents, which was gathered in 2018 from China Family Panel Studies(CFPS). Data were analyze using SPSS 26 to get the descriptive statistic and nested model result. Result found, the educational expectations of the teenagers are somewhat influenced by their home background and the school environment.
Diagnostic and prognostic value of time to positivity in blood cultures. An opinion paper
Authors: Maffezzoli Paola , Kestler Martha , Burillo Almudena , Corcione Silvia , De Rosa Francesco Giuseppe , Muñoz Patricia , Bouza Emilio
Published: November 2024

Time to positivity (TTP) refers to the duration required for a microbiological culture test to indicate a positive result, marking the onset of detectable bacterial or fungal growth in the sample. Numerous variables, including patient characteristics, infection source, former antimicrobial therapy, blood sample volume, and sample transportation time can influence the value of TTP. Several studies have been conducted on bloodstream infections, whereas studies on the clinical significance of yeast TTP are quite limited in the literature. Furthermore, many studies are retrospective and have a small sample size. In this opinion paper, we have formulated some questions and attempted to provide answers based on the available literature and our perspective. The objective of this opinion paper is to summarise current knowledge based on the literature, aiming to offer a critical perspective, particularly on aspects with weaker evidence, which could guide future studies in this area. We believe that TTP of blood cultures appears to exhibit considerable potential and may prove to be a valuable tool in clinical practice for estimating patient mortality risk and guiding antimicrobial therapy choices. Topics discussed include the diagnostic and prognostic role of TTP in Gram-positive and Gram-negative bacteremias and in candidemias, and the significance of differential time to positivity (DTTP). In summary, our opinion is that, based on the available literature, it is not possible to determine whether TTP provides prognostic information, particularly concerning candidemia. Therefore, clinical decisions cannot be systematically based on this parameter.
recuperación de los costes de producción y suministro de aguas residuales regeneradas
Authors: Arimany Lamoglia Esteban
Published: November 2024

La regeneración de aguas residuales y el suministro de las regeneradas para ser reutilizadas genera costes. El agua residual regenerada constituye una nueva fuente de recursos hídricos cuyo aprovechamiento debe promoverse para, entre otras cosas, disminuir la presión sobre los recursos convencionales. Ahora bien, será difícil conseguir los objetivos si normativamente no se acierta en la determinación de quien debe soportar los costes de producción y suministro y de los mecanismos adecuados para recuperarlos.
HISTOLOGICAL EVALUATION OF THE EFFECTS OF ACTION BITTERS ON THE TESTES OF ADULT MALE WISTAR RATS
Authors:   Department of Human Anatomy and Cell Biology, Delta State University, Abraka, Nigeria, Santos Ehebha Ehizokhale , Sylvia Ogwu Chineye ,   Department of Human Anatomy and Cell Biology, Delta State University, Abraka, Nigeria, Kennedy Obohwemu Oberhiri ,   Department of Health, Wellbeing & Social Care, Global Banking School/Oxford Brookes University, Birmingham, United Kingdom; PENKUP Research Institute, Birmingham, United Kingdom, Pearl Egbowawa Ufuoma ,   Department of Environmental Toxicology and Public Health, Federal University of Petroleum Resources, Effurun, Delta State, Nigeria
Published: November 2024

Action Bitters, a popular herbal concoction, has been traditionally used to treat various health conditions, including those related to the male reproductive system. Despite its widespread use, there is a dearth of scientific evidence on its effects on the testes. This study aimed to investigate the histological changes in the testes of adult male Wistar rats following administration of Action Bitters. Methods: Fifteen adult male Wistar rats were randomly divided into three groups (n = 5). Group A served as the normal control, while groups B and C received 10% (0.66ml/kg) and 5% (0.33ml/kg) lethal dose 50 (Ld50) of Action Bitters, respectively. The Ld50 values were calculated based on the median lethal dose of the herbal remedy. The rats were administered the herbal remedy for a period of one month, after which they were sacrificed, and their testes collected for histological examination. Results: Microscopic examination of the testicular tissue samples revealed no evidence of abnormal cellular changes or tissue damage. The tissue samples appeared normal, with no signs of inflammation, necrosis, or apoptosis. Discussion: The findings of this study suggest that Action Bitters does not induce histological changes in the testes of adult male Wistar rats. These results provide new insights into the potential mechanism of action of the herbal medication on testicular function. The study highlights the importance of considering both histological and biochemical assessments when evaluating the effects of herbal remedies on reproductive health. Conclusion: This study contributes to the growing body of knowledge on the effects of herbal remedies on the male reproductive system. The findings suggest that Action Bitters may be a safe and effective herbal remedy for treating reproductive health issues, and further research is warranted to explore its potential benefits and mechanisms of action.