bluuebunny/crossref_metadata_2025 huggingface dataset:
A miniaturized multicellular platform to mimic the 3D structure of the alveolar-capillary barrier
Authors: Licciardello Michela , Traldi Cecilia , Cicolini Martina , Bertana Valentina , Marasso Simone Luigi , Cocuzza Matteo , Tonda-Turo Chiara , Ciardelli Gianluca
Published: April 2024

Several diseases affect the alveoli, and the efficacy of medical treatments and pharmaceutical therapies is hampered by the lack of pre-clinical models able to recreatein vitrothe diseases. Microfluidic devices, mimicking the key structural and compositional features of the alveoli, offer several advantages to medium and high-throughput analysis of new candidate therapies. Here, we developed an alveolus-on-a-chip recapitulating the microanatomy of the physiological tissue by including the epithelium, the fibrous interstitial layer and the capillary endothelium. A PDMS device was obtained assembling a top layer and a bottom layer obtained by replica molding. A polycaprolactone/gelatin (PCL-Gel) electrospun membrane was included within the two layers supporting the seeding of 3 cell phenotypes. Epithelial cells were grown on a fibroblast-laden collagen hydrogel located on the top side of the PCL-Gel mats while endothelial cells were seeded on the basolateral side of the membrane. The innovative design of the microfluidic device allows to replicate both cell-cell and cell-extracellular matrix interactions according to thein vivocell arrangement along with the establishment of physiologically relevant air-liquid interface conditions. Indeed, high cell viability was confirmed for up to 10 days and the formation of a tight endothelial and epithelial barrier was assessed by immunofluorescence assays.
A synthetic biology approach for the treatment of pollutants with microalgae
Authors: Webster Luke J. , Villa-Gomez Denys , Brown Reuben , Clarke William , Schenk Peer M.
Published: April 2024

The increase in global population and industrial development has led to a significant release of organic and inorganic pollutants into water streams, threatening human health and ecosystems. Microalgae, encompassing eukaryotic protists and prokaryotic cyanobacteria, have emerged as a sustainable and cost-effective solution for removing these pollutants and mitigating carbon emissions. Various microalgae species, such as C. vulgaris, P. tricornutum, N. oceanica, A. platensis, and C. reinhardtii, have demonstrated their ability to eliminate heavy metals, salinity, plastics, and pesticides. Synthetic biology holds the potential to enhance microalgae-based technologies by broadening the scope of treatment targets and improving pollutant removal rates. This review provides an overview of the recent advances in the synthetic biology of microalgae, focusing on genetic engineering tools to facilitate the removal of inorganic (heavy metals and salinity) and organic (pesticides and plastics) compounds. The development of these tools is crucial for enhancing pollutant removal mechanisms through gene expression manipulation, DNA introduction into cells, and the generation of mutants with altered phenotypes. Additionally, the review discusses the principles of synthetic biology tools, emphasizing the significance of genetic engineering in targeting specific metabolic pathways and creating phenotypic changes. It also explores the use of precise engineering tools, such as CRISPR/Cas9 and TALENs, to adapt genetic engineering to various microalgae species. The review concludes that there is much potential for synthetic biology based approaches for pollutant removal using microalgae, but there is a need for expansion of the tools involved, including the development of universal cloning toolkits for the efficient and rapid assembly of mutants and transgenic expression strains, and the need for adaptation of genetic engineering tools to a wider range of microalgae species.
ASCL1 promotes Scrt2 expression in the neural tube
Authors: Goes Carolina Purcell , Botezelli Vitória Samartin , De La Cruz Shirley Mirna , Cruz Mário Costa , Azambuja Ana Paula , Simoes-Costa Marcos , Yan Chao Yun Irene
Published: April 2024

ASCL1 is a transcription factor that directs neural progenitors towards lineage differentiation. Although many of the molecular mechanisms underlying its action have been described, several of its targets remain unidentified. We identified in the chick genome a putative enhancer (cE1) upstream of the transcription factor Scratch2 (Scrt2) locus with a predicted heterodimerization motif for ASCL1 and POU3F2. In this study, we investigated the role of ASCL1 and this enhancer in regulating the expression of the Scrt2 in the embryonic spinal cord. We confirmed that cE1 region interacted with the Scrt2 promoter. cE1 was sufficient to mediate ASCL1-driven expression in the neural tube through the heterodimerization sites. Moreover, Scrt2 expression was inhibited when we removed cE1 from the genome. These findings strongly indicate that ASCL1 regulates Scrt2 transcription in the neural tube through cE1.
Transforming informal communication in the urgent shift to teleworking: a case study in Japan
Authors: Watanabe Kentaro , Umemura Hiroyuki , Mori Ikue , Amemiya Saya , Yamamoto Yuji
Published: April 2024

Introduction

While informal communication is essential for employee performance and wellbeing, it is difficult to maintain in telework settings. This issue has recently been becoming more prominent worldwide, especially because of the coronavirus disease 2019 (COVID-19). Nevertheless, how employees managed their informal communication in the sudden shift to teleworking is still understudied. This study fills this research gap by clarifying how an organization's employees improvised informal communication during the urgent shift to teleworking.

Methods

An exploratory case study of a large construction firm in Japan was conducted, focusing on how employees improvised informal communication during teleworking in response to COVID-19. The authors conducted semi-structured interviews with 24 employees and applied a qualitative thematic analysis to the collected data.

Results

Four informal communication strategies (structured informality, casual tech, implicit communication, and face-to-face opportunity seeking) that were improvised amid the sudden shift to teleworking caused by COVID-19 were identified.

Discussion

The findings can inform concrete means for the effective and dynamic transition of informal communication to teleworking settings during emergencies, thus contributing to informal communication studies as well as the promotion of resilient business operations and employee wellbeing in response to future crises.
A comprehensive evaluation of marker-based, markerless methods for loose garment scenarios in varying camera configurations
Authors: Ray Lala Shakti Swarup , Zhou Bo , Suh Sungho , Lukowicz Paul
Published: April 2024

In support of smart wearable researchers striving to select optimal ground truth methods for motion capture across a spectrum of loose garment types, we present an extended benchmark named DrapeMoCapBench (DMCB+). This augmented benchmark incorporates a more intricate limb-wise Motion Capture (MoCap) accuracy analysis, and enhanced drape calculation, and introduces a novel benchmarking tool that encompasses multicamera deep learning MoCap methods. DMCB+ is specifically designed to evaluate the performance of both optical marker-based and markerless MoCap techniques, taking into account the challenges posed by various loose garment types. While high-cost marker-based systems are acknowledged for their precision, they often require skin-tight markers on bony areas, which can be impractical with loose garments. On the other hand, markerless MoCap methods driven by computer vision models have evolved to be more cost-effective, utilizing smartphone cameras and exhibiting promising results. Utilizing real-world MoCap datasets, DMCB+ conducts 3D physics simulations with a comprehensive set of variables, including six drape levels, three motion intensities, and six body-gender combinations. The extended benchmark provides a nuanced analysis of advanced marker-based and markerless MoCap techniques, highlighting their strengths and weaknesses across distinct scenarios. In particular, DMCB+ reveals that when evaluating casual loose garments, both marker-based and markerless methods exhibit notable performance degradation (>10 cm). However, in scenarios involving everyday activities with basic and swift motions, markerless MoCap outperforms marker-based alternatives. This positions markerless MoCap as an advantageous and economical choice for wearable studies. The inclusion of a multicamera deep learning MoCap method in the benchmarking tool further expands the scope, allowing researchers to assess the capabilities of cutting-edge technologies in diverse motion capture scenarios.
The role of tubular cells in the pathogenesis of Fabry nephropathy
Authors: Rozenfeld Paula , Feriozzi Sandro , Braun Fabian
Published: April 2024

The pathophysiology of Fabry nephropathy (FN) is induced by galactosidase A deficiency with a chronic exposure of glycolipids to every lineage of renal cells. Tissue damage is attributed to the activation of molecular pathways, resulting in tissue fibrosis and chronic kidney disease. Podocytes have been the primary focus in clinical pathophysiological research because of the striking accumulation of large glycolipid deposits observable in histology. Yet, the tubular interstitium makes up a large portion of the whole organ, and therefore, its role must be further considered in pathogenic processes. In this review, we would like to propose Fabry tubulopathy and its ensuing functional effects as the first pathological signs and contributing factors to the development of FN. We will summarize and discuss the current literature regarding the role of tubular cells in Fabry kidney pathophysiology. Starting from clinical and histological evidence, we will highlight the data from animal models and cell cultures outlining the pathophysiological pathways associated with tubular interstitial injury causing renal fibrosis in Fabry nephropathy.
An adaptive energy management strategy for airports to achieve carbon neutrality by 2050 via waste, wind, and solar power
Authors: Goh Hui Hwang , Suo Wenxuan , Liang Xue , Zhang Dongdong , Dai Wei , Kurniawan Tonni Agustiono , Goh Kai Chen
Published: April 2024

After the border lockdown, global traffic has returned, making green airports a government goal to reach carbon neutrality by 2050. Sustainable aviation fuel (SAF) use for commercial aircraft alone won't help achieve net-zero emissions (NEZ). An independent renewable energy supply system at airports is urgently needed to implement green airports worldwide. This study develops a renewable energy power supply system that integrates wind, photovoltaic (PV), and waste-to-energy (WTE) sources to investigate a new adaptive model predictive control (MPC) energy management strategy based on airport operational characteristics. Simulations of Copenhagen Airport’s energy programme confirmed this energy management strategy’s feasibility. Energy management scenarios for two typical summer and winter load days were simulated for case studies. Field data from Copenhagen was used for simulation analysis, and experimental modelling of the airport’s energy scheme proved the energy management program’s practicality. The simulations show that Copenhagen airport renewable energy generates half of its electricity in winter and 81.0% in summer. A total operating cost of USD 36,087.83/day in summer and USD 102,061.20/day in winter is needed to reduce carbon emissions by 41.19 Mt/day in summer and 43.96 Mt/day in winter. This approach should help deploy green airports worldwide and reach carbon neutrality by 2050.
Gross ecosystem product accounting in Miyun County: the supply and use of ecosystem services
Authors: Shen Yu-qian , Yi Xiao , Chen Meng , Ouyang Zhi-yun
Published: April 2024

The ability of an ecosystem to provide services differs from its actual consumption and use by human society. Overuse of ecosystems can degrade ecosystems. In order to evaluate the supply capacity and use status of the ecosystem in Miyun County, so as to better apply the ecosystem services to different policies and management, the supply and use of 10 types of ecosystem services in Miyun County were calculated by using value indicators, namely potential gross ecosystem product (GEP) and actual gross ecosystem product. The results show that: (1) In 2020, potential gross ecosystem product of Miyun County is 254.32 billion yuan, and actual gross ecosystem product is 53.28 billion yuan, accounting for 21% of the potential gross ecosystem product. (2) Among all kinds of ecosystem services, the contribution of water conservation services in potential gross ecosystem product is the highest, and the contribution of climate regulation services in actual gross ecosystem product is the highest. (3) The contribution of wetland was the highest in potential gross ecosystem product, while the contribution of forest was the highest in actual gross ecosystem product. (4) Natural ecosystem area and vegetation coverage are the main factors affecting potential gross ecosystem product, while actual gross ecosystem product is mainly affected by GDP and population. (5) By studying the potential supply and actual use of ecosystem services, we evaluated and distinguished between the services that ecosystem could produce and the services that were actually used by humans, and compared the supply capacity and actual use to assess the sustainability of ecosystem services, in order to formulate different policies and management measures for gross ecosystem product surplus and deficit regions. Potential gross ecosystem product could provide data support for the assessment of ecological protection benefits. Actual gross ecosystem product could be included in the system of national accounts, and as a standard for ecological compensation and ecological trading. Confusing potential supply and actual use in policy application would affect the effect of policy implementation, and distinguishing them could ensure policy implementation. The relative size of the relationship between the two could reflect the present and future levels of human well-being in a region. Both together provided management basis and policy-making support for guiding regional ecological protection and sustainable development.
Immunoglobulins and serum proteins impair anti-tumor NK cell effector functions in malignant ascites
Authors: Hrvat Antonio , Benders Sonja , Kimmig Rainer , Brandau Sven , Mallmann-Gottschalk Nina
Published: April 2024

Introduction

Malignant ascites indicates ovarian cancer progression and predicts poor clinical outcome. Various ascites components induce an immunosuppressive crosstalk between tumor and immune cells, which is poorly understood. In our previous study, imbalanced electrolytes, particularly high sodium content in malignant ascites, have been identified as a main immunosuppressive mechanism that impaired NK and T-cell activity.

Methods

In the present study, we explored the role of high concentrations of ascites proteins and immunoglobulins on antitumoral NK effector functions. To this end, a coculture system consisting of healthy donor NK cells and ovarian cancer cells was used. The anti-EGFR antibody Cetuximab was added to induce antibody-dependent cellular cytotoxicity (ADCC). NK activity was assessed in the presence of different patient ascites samples and immunoglobulins that were isolated from ascites.

Results

Overall high protein concentration in ascites impaired NK cell degranulation, conjugation to tumor cells, and intracellular calcium signaling. Immunoglobulins isolated from ascites samples competitively interfered with NK ADCC and inhibited the conjugation to target cells. Furthermore, downregulation of regulatory surface markers CD16 and DNAM-1 on NK cells was prevented by ascites-derived immunoglobulins during NK cell activation.

Conclusion

Our data show that high protein concentrations in biological fluids are able to suppress antitumoral activity of NK cells independent from the mechanism mediated by imbalanced electrolytes. The competitive interference between immunoglobulins of ascites and specific therapeutic antibodies could diminish the efficacy of antibody-based therapies and should be considered in antibody-based immunotherapies.
Microglial- neuronal crosstalk in chronic viral infection through mTOR, SPP1/OPN and inflammasome pathway signaling
Authors: Argandona Lopez Catalina , Brown Amanda M.
Published: April 2024

HIV-infection of microglia and macrophages (MMs) induces neuronal injury and chronic release of inflammatory stimuli through direct and indirect molecular pathways. A large percentage of people with HIV-associated neurologic and psychiatric co-morbidities have high levels of circulating inflammatory molecules. Microglia, given their susceptibility to HIV infection and long-lived nature, are reservoirs for persistent infection. MMs and neurons possess the molecular machinery to detect pathogen nucleic acids and proteins to activate innate immune signals. Full activation of inflammasome assembly and expression of IL-1β requires a priming event and a second signal. Many studies have demonstrated that HIV infection alone can activate inflammasome activity. Interestingly, secreted phosphoprotein-1 (SPP1/OPN) expression is highly upregulated in the CNS of people infected with HIV and neurologic dysfunction. Interestingly, all evidence thus far suggests a protective function of SPP1 signaling through mammalian target of rapamycin (mTORC1/2) pathway function to counter HIV-neuronal injury. Moreover, HIV-infected mice knocked down for SPP1 show by neuroimaging, increased neuroinflammation compared to controls. This suggests that SPP1 uses unique regulatory mechanisms to control the level of inflammatory signaling. In this mini review, we discuss the known and yet-to-be discovered biological links between SPP1-mediated stimulation of mTOR and inflammasome activity. Additional new mechanistic insights from studies in relevant experimental models will provide a greater understanding of crosstalk between microglia and neurons in the regulation of CNS homeostasis.
The state of the art in the treatment of severe aplastic anemia: immunotherapy and hematopoietic cell transplantation in children and adults
Authors: Piekarska Agnieszka , Pawelec Katarzyna , Szmigielska-Kapłon Anna , Ussowicz Marek
Published: April 2024

Acquired aplastic anemia (AA) is an immune-mediated bone marrow (BM) failure where marrow disruption is driven by a cytotoxic T-cell–mediated autoimmune attack against hematopoietic stem cells. The key diagnostic challenge in children, but also in adults, is to exclude the possible underlying congenital condition and myelodysplasia. The choice of treatment options, either allogeneic hematopoietic cell transplantation (alloHCT) or immunosuppressive therapy (IST), depends on the patient’s age, comorbidities, and access to a suitable donor and effective therapeutic agents. Since 2022, horse antithymocyte globulin (hATG) has been available again in Europe and is recommended for IST as a more effective option than rabbit ATG. Therefore, an update on immunosuppressive strategies is warranted. Despite an improved response to the new immunosuppression protocols with hATG and eltrombopag, some patients are not cured or remain at risk of aplasia relapse or clonal evolution and require postponed alloHCT. The transplantation field has evolved, becoming safer and more accessible. Upfront alloHCT from unrelated donors is becoming a tempting option. With the use of posttransplant cyclophosphamide, haploidentical HCT offers promising outcomes also in AA. In this paper, we present the state of the art in the management of severe AA for pediatric and adult patients based on the available guidelines and recently published studies.
Seasonal variability of coastal pH and CO2 using an oceanographic buoy in the Canary Islands
Authors: González Aridane G. , Aldrich-Rodríguez Ariadna , González-Santana David , González-Dávila Melchor , Santana-Casiano J. Magdalena
Published: April 2024

Ocean acidification, caused by the absorption of carbon dioxide (CO2) from the atmosphere into the ocean, ranks among the most critical consequences of climate change for marine ecosystems. Most studies have examined pH and CO2 trends in the open ocean through oceanic time-series research. The analysis in coastal waters, particularly in island environments, remains relatively underexplored. This gap in our understanding is particularly important given the profound implications of these changes for coastal ecosystems and the blue economy. The present study focuses on the ongoing monitoring effort that started in March 2020 along the east coast of Gran Canaria, within the Gando Bay, by the CanOA-1 buoy. This monitoring initiative focuses on the systematic collection of multiple variables within the CO2 system, such as CO2 fugacity (fCO2), pH (in total scale, pHT), total inorganic carbon (CT), and other hydrographic variables including sea surface salinity (SSS), sea surface temperature (SST) and wind intensity and direction. Accordingly, the study allows the computation of the CO2 flux (FCO2) between the surface waters and the atmosphere. During the study period, stational (warm and cold periods) behavior was found for all the variables. The lowest SST values were recorded in March, with a range of 18.8-19.3°C, while the highest SST were observed in September and October, ranging from 24.5-24.8°C. SST exhibited an annual increase with a rate of 0.007°C yr-1. Warmer months increased SSS, while colder periods, influenced by extreme events like tropical storms, led to lower salinity (SSS=34.02). The predominant Trade Winds facilitated the arrival of deeper water, replenishing seawater. The study provided insights into atmospheric CO2. Atmospheric fCO2 averaged 415 ± 4 µatm (2020-2023). Surface water fCO2sw presented variability, with the highest values recorded in September and October, peaking at 437 µatm in September 2021. The lowest values for fCO2sw were found in February 2021 (368 µatm). From 2020 to 2023, surface water fCO2sw values displayed an increasing rate of 1.9 µatm yr-1 in the study area. The assessment of fCO2sw decomposition into thermal and non-thermal processes revealed the importance of SST on the fCO2sw. Nevertheless, in the present study, it is crucial to remark the impact of non-thermal factors on near-shallow coastal regions. Our findings highlight the influence of physical factors such as tides, and wind effect to horizontal mixing in these areas. The CT showed a mean concentration of 2113 ± 8 μmol kg-1 and pH at in-situ temperature (pHT,IS) has a mean value of 8.05 ± 0.02. The mean FCO2 from 2020 to 2023 was 0.34 ± 0.04 mmol m-2 d-1 (126 ± 13 mmol m-2 yr-1) acting as a slight CO2 source. In general, between May and December were the months when the area was a source of CO2. Extrapolating to the entire 6 km2 of Gando Bay, the region sourced 33 ± 4 Tons of CO2 yr-1.
Community changes of gut microbes highlight their importance in the adaptation of copepods to toxic dinoflagellates
Authors: Yang Jing , Xu Zhimeng , Chen Yi , Xu Huo , Gao Zuyuan , Zhang Xiaodong , Pang Mengwen , Zhang Shuwen , Liu Hongbin
Published: April 2024

Zooplankton grazers, like copepods, can feed on toxic microalgae and live normally. We hypothesize that gut microbial communities (GMCs) may contribute to the detoxification of the host by changing their compositions and recruiting more beneficial bacteria. Here, we measured the physiological responses of two copepod species (Acartia sp. and Paracalanus sp.) fed with toxic (Alexandrium tamarense) and non-toxic (Alexandrium andersonii) dinoflagellates, respectively. Both copepods maintained consistently high survival rates but slightly reduced ingestion rates when feeding upon the toxic dinoflagellate (when compared to the non-toxic one), suggesting a compensatory mechanism. The compositional variation of copepod GMCs, at the amplicon sequence variant (ASV) level, was mostly significantly different among copepod host species (R = 0.83, by ANOSIM test), while diet type played minor but significant roles. Under the toxic diet, Acartia sp. enriched only five ASVs while Paracalanus sp. recruited a wide range of taxa (38 ASVs) mostly belonging to Alphaproteobacteria (e.g., Rhodobacteraceae) and Gammaproteobacteria (e.g., Alteromonadaceae). In contrast, when clustering GMCs by predicted functions, diet type was the key regulating factor, suggesting the functional convergence of copepod GMCs in response to algal toxins. This can be explained by the fact that most of the enriched bacteria under the toxic diet have similar functions on detoxification and maintaining the host homeostasis. This study deepens our understanding of the roles of GMC in the detoxification and adaptation mechanisms of copepods during harmful algal blooms.
Strategic insights in manufacturing site selection: a multi-method approach using factor rating, analytic hierarchy process, and best worst method
Authors: Sharma Parveen , Singh Ghatorha Kashmir , Kang Amardeep Singh , Cepova Lenka , Kumar Ajay , Phanden Rakesh Kumar
Published: April 2024

The current study focuses on selecting the most suitable site location for a manufacturing industry using the Factor Rating Method (FRM). The study considers six key factors: Raw Materials Availability, Location, Availability of Labor, Transport, Availability of Utilities, and Environmental Impact. The FRM assign weights to each factor based on their relative importance. The results indicate that Raw Materials Availability holds the highest weight, suggesting its critical influence on site selection decisions. Subsequently, the Analytic Hierarchy Process (AHP) and Best Worst Method (BWM) are utilized to prioritize three available location alternatives through pairwise criteria comparisons. The analysis reveals that Location C emerges as the most favorable option, effectively meeting the manufacturing industry’s requirements. The successful application of these methods demonstrates their value in aiding decision-making processes related to site location selection. By considering multiple factors and utilizing structured methodologies, organizations can make informed choices aligned with their specific needs and goals. This research contributes to the existing body of knowledge by providing insights into effective site selection strategies for the manufacturing industry. Further research opportunities exist in incorporating additional factors, addressing real-world constraints, and conducting sensitivity analyses to enhance the accuracy and applicability of site location decision-making.
Evaluation of bacterial safety approaches of platelet blood concentrates: bacterial screening and pathogen reduction
Authors: Rezvany Mohammad Reza , Moradi Hasan-Abad Amin , Sobhani-Nasab Ali , Esmaili Mohammad Ali
Published: April 2024

This mini-review analyzed two approaches to screening bacterial contamination and utilizing pathogen reduction technology (PRT) for Platelet concentrates (PCs). While the culture-based method is still considered the gold standard for detecting bacterial contamination in PCs, efforts in the past two decades to minimize transfusion-transmitted bacterial infections (TTBIs) have been insufficient to eliminate this infectious threat. PRTs have emerged as a crucial tool to enhance safety and mitigate these risks. The evidence suggests that the screening strategy for bacterial contamination is more successful in ensuring PC quality, decreasing the necessity for frequent transfusions, and improving resistance to platelet transfusion. Alternatively, the PRT approach is superior regarding PC safety. However, both methods are equally effective in managing bleeding. In conclusion, PRT can become a more prevalent means of safety for PCs compared to culture-based approaches and will soon comprehensively surpass culture-based bacterial contamination detection methods.
Risk factors for anxiety and its impacts on acute exacerbation in older patients with chronic obstructive pulmonary disease
Authors: Mou Yan , Shan Lin , Liu Yunhuan , Wang Yue , He Zhengming , Li Xiangyang , Zhu Huili , Ge Haiyan
Published: April 2024

Background

Anxiety is common in patients with chronic obstructive pulmonary disease (COPD), especially in older patients with the definition of age over 60 years old. Few studies have focused on anxiety in older COPD patients. This study aimed to analyze the risk factors of anxiety in older COPD patients and the impacts of anxiety on future acute exacerbation.

Methods

The general information, questionnaire data, previous acute exacerbation and pulmonary function were collected. Hamilton Anxiety Rating Scale (HAMA) was used to evaluate the anxiety of older COPD patients. The patients were followed up for one year, the number and the degrees of acute exacerbations of COPD were recorded.

Results

A total of 424 older COPD patients were included in the analysis. 19.81% (N = 84) had anxiety symptoms, and 80.19% (N = 340) had no anxiety symptoms. There were increased pack-years, more comorbidities, and more previous acute exacerbations in older COPD patients with anxiety compared to those without anxiety (P < 0.05). Meanwhile, a higher modified Medical Research Council (mMRC), a higher COPD assessment test (CAT) score and a shorter six-minute walking distance (6MWD) were found in older COPD patients with anxiety (P < 0.05). The BODE index, mMRC, CAT score, comorbidities and acute exacerbations were associated with anxiety. Eventually, anxiety will increase the risk of future acute exacerbation in older COPD patients (OR = 4.250, 95% CI: 2.369–7.626).

Conclusion

Older COPD patients with anxiety had worsening symptoms, more comorbidities and frequent acute exacerbation. Meanwhile, anxiety may increase the risk of acute exacerbation in the future. Therefore, interventions should be provided to reduce the risk of anxiety in older COPD patients at an early stage.
Harnessing rhizospheric core microbiomes from arid regions for enhancing date palm resilience to climate change effects
Authors: Ben Zineb Ameni , Lamine Mariem , Khallef Ahlem , Hamdi Helmi , Ahmed Talaat , Al-Jabri Hareb , Alsafran Mohammed , Mliki Ahmed , Sayadi Sami , Gargouri Mahmoud
Published: April 2024

Date palm cultivation has thrived in the Gulf Cooperation Council region since ancient times, where it represents a vital sector in agricultural and socio-economic development. However, climate change conditions prevailing for decades in this area, next to rarefication of rain, hot temperatures, intense evapotranspiration, rise of sea level, salinization of groundwater, and intensification of cultivation, contributed to increase salinity in the soil as well as in irrigation water and to seriously threaten date palm cultivation sustainability. There are also growing concerns about soil erosion and its repercussions on date palm oases. While several reviews have reported on solutions to sustain date productivity, including genetic selection of suitable cultivars for the local harsh environmental conditions and the implementation of efficient management practices, no systematic review of the desertic plants’ below-ground microbial communities and their potential contributions to date palm adaptation to climate change has been reported yet. Indeed, desert microorganisms are expected to address critical agricultural challenges and economic issues. Therefore, the primary objectives of the present critical review are to (1) analyze and synthesize current knowledge and scientific advances on desert plant-associated microorganisms, (2) review and summarize the impacts of their application on date palm, and (3) identify possible gaps and suggest relevant guidance for desert plant microbes’ inoculation approach to sustain date palm cultivation within the Gulf Cooperation Council in general and in Qatar in particular.
Love-hate relationship between hepatitis B virus and type 2 diabetes: a Mendelian randomization study
Authors: Yu Yunfeng , Tong Keke , Hu Gang , Yang Xinyu , Wu Jingyi , Bai Siyang , Yu Rong
Published: April 2024

Objective

The impact of hepatitis B virus (HBV) on the risk of type 2 diabetes (T2D) remains a controversial topic. This study aims to analyze the causal relationship between HBV and T2D using Mendelian randomization (MR).

Methods

Single nucleotide polymorphisms on chronic hepatitis B (CHB), liver fibrosis, liver cirrhosis, and T2D were obtained from BioBank Japan Project, European Bioinformatics Institute, and FinnGen. Mendelian randomization was utilized to evaluate exposure-outcome causality. Inverse variance weighted was used as the primary method for MR analysis. To assess horizontal pleiotropy and heterogeneity, we conducted MR-Egger intercept analysis and Cochran’s Q test, and the robustness of the MR analysis results was evaluated through leave-one-out sensitivity analysis.

Results

MR analysis revealed that CHB was associated with a decreased genetic susceptibility to T2D (OR, 0.975; 95% CI, 0.962–0.989; p < 0.001) while liver cirrhosis (OR, 1.021; 95% CI, 1.007–1.036; p = 0.004) as well as liver cirrhosis and liver fibrosis (OR, 1.015; 95% CI, 1.002–1.028; p = 0.020) were associated with an increased genetic susceptibility to T2D. MR-Egger intercept showed no horizontal pleiotropy (p > 0.05). Cochran’s Q showed no heterogeneity (p > 0.05). Leave-one-out sensitivity analysis showed that the results were robust.

Conclusion

CHB has the potential to act as a protective factor for T2D, but its effectiveness is constrained by viral load and disease stage. This protective effect diminishes or disappears as viral load decreases, and it transforms into a risk factor with the progression to liver fibrosis and cirrhosis.
Overview of Proteus mirabilis pathogenicity and virulence. Insights into the role of metals
Authors: Chakkour Mohamed , Hammoud Zeinab , Farhat Solay , El Roz Ali , Ezzeddine Zeinab , Ghssein Ghassan
Published: April 2024

Proteus mirabilis is a Gram-negative bacterium with exclusive molecular and biological features. It is a versatile pathogen acclaimed for its distinct urease production, swarming behavior, and rapid multicellular activity. Clinically, P. mirabilis is a frequent pathogen of the human urinary system where it causes urinary tract infections (UTIs) and catheter-associated urinary tract infections (CAUTIs). This review explores the epidemiology, risk factors, clinical manifestations, and treatment of P. mirabilis infections, emphasizing its association with UTIs. The bacterium’s genome analysis revealed the presence of resistance genes against commonly used antibiotics, an antibiotic-resistant phenotype that poses a serious clinical challenge. Particularly, the emergence of extended-spectrum β-lactamases (ESBLs) and carbapenemases resistant P. mirabilis strains. On a molecular level, P. mirabilis possesses a wide array of virulence factors including the production of fimbriae, urease, hemolysins, metallophores, and biofilm formation. This review thoroughly tackles a substantial gap in understanding the role of metallophores in shaping the virulence factors of P. mirabilis virulence. Siderophores, iron metal chelating and transporting metallophores, particularly contribute to the complex pathogenic strategies, displaying a potential target for therapeutic intervention.
Optimization method for human-robot command combinations of hexapod robot based on multi-objective constraints
Authors: Chen Xiaolei , You Bo , Dong Zheng
Published: April 2024

Due to the heavy burden on human drivers when remotely controlling hexapod robots in complex terrain environments, there is a critical need for robot intelligence to assist in generating control commands. Therefore, this study proposes a mapping process framework that generates a combination of human-robot commands based on decision target values, focusing on the task of robot intelligence assisting drivers in generating human-robot command combinations. Furthermore, human-robot state constraints are quantified as geometric constraints on robot motion and driver fatigue constraints. By optimizing and filtering the feasible set of human-robot commands based on human-robot state constraints, instruction combinations are formed and recommended to the driver in real-time, thereby enhancing the efficiency and safety of human-machine coordination. To validate the effectiveness of the proposed method, a remote human-robot collaborative driving control system based on wearable devices is designed and implemented. Experimental results demonstrate that drivers utilizing the human-robot command recommendation system exhibit significantly improved robot walking stability and reduced collision rates compared to individual driving.
The interpositional bypass with a parietal branch of superficial temporal artery graft for symptomatic atherosclerotic anterior cerebral artery stenosis or occlusion
Authors: Tai Chuyang , Ling Cong , Huang Tengchao , Zhang Baoyu , Yang Yang , Wei Lei , Wu Haiwan , Mo Ni , Wang Hui , Chen Chuan
Published: April 2024

Background

For nonmoyamoya patients with anterior cerebral artery (ACA) stenosis or occlusion, whether direct revascularization of the ACA territory can prevent stroke is still unclear. The objective of this study was to investigate the efficacy and safety of a parietal branch of superficial temporal artery-interposed superficial temporal artery-to-ACA bypass (PISAB) for preventing stroke in patients with symptomatic atherosclerotic ACA stenosis or occlusion (SAASO).

Methods

We retrospectively analyzed the data from patients with SAASO who had undergone PISAB in our center between April 2016 and November 2021. The rates of patency, satisfaction (revascularization grades A and B) of bypass, perioperative complications, recurrence of ischemic stroke, changes in bypass flow, and improvements in cerebral blood perfusion were analyzed.

Results

A total of 19 SAASO patients were involved in this study. Sixteen out of 19 (84.2%) patients were free from any cerebral ischemic events after surgery. Only 3 patients (15.8%) had recurrent stroke postoperatively. Two (10.5%) surgery-related complications occurred, including hyperperfusion syndrome and minor stroke. No skin ischemic complications occurred. The average follow-up period was 50.6 ± 18.3 months. The flow rate of the bypass was significantly increased half a year after surgery (56.2 ± 8.0 mL/min vs. 44.3 ± 5.3 mL/min, p < 0.001). The ratio of ipsilateral/contralateral mean transit time in the superior frontal gyrus was decreased significantly after bypass (1.08 ± 0.07 vs. 1.23 ± 0.05, p < 0.001) and continued to decrease 6 months after surgery (1.05 ± 0.04 vs. 1.08 ± 0.07, p = 0.002). The patency rate of PISAB was 94.7% (18/19) 2 years after surgery. The satisfaction rate of bypass was 89.5% (17/19).

Conclusion

The results of this study indicate that PISAB, as a safe superficial bypass, can effectively reduce the risk of stroke in SAASO patients. More precise conclusions will require randomized control studies.
Household food security status and its associated factors among pensioners in Arba Minch town, South Ethiopia
Authors: Mamo Daniel Niguse , Worku Kassahun Misgana , Adem Yonas Fissha , Shibabaw Adamu Ambachew , Habte Aklilu , Haile Yosef
Published: April 2024

Introduction

Food insecurity has remained a serious public health problem in developing countries, such as Ethiopia, over the past two decades. Vulnerable populations, such as pensioners, have been affected by this problem because of emerging socio-demographic changes, a global financial crisis, and climate change, all of which have contributed to the high food prices. Hence, this study aimed to assess household food security status and associated factors among pensioners in Arba Minch town, South Ethiopia.

Methods

A community-based cross-sectional study design was conducted from September to October 2023. Two hundred forty-four pensioners were chosen using a simple random sampling technique. Data were collected, cleaned, and entered into EPI-Data version 4.6 and exported to SPSS version 25 for analysis. Variables with a p-value of ≤0.25 in the bivariate analyses were candidates for the multivariable regression analysis. In the multivariable logistic regression, variables with a p-value of 0.05 were considered to have a significant association with the dependent variable.

Results

A total of 238 retired people were interviewed, with a response rate of 97.5%. Among the interviewed pensioners, 223 (91.4%) households were food insecure. Having more than one dependent member [AOR = 2.4, 95% C.I: 1.30, 6.64], being jobless after retirement [AOR = 3, 95% C.I:1.17, 5.61], and being in the lowest tertile of wealth status [AOR = 2, 95% C.I:1.36, 4.99] were identified as predictors of food insecurity.

Conclusion

The magnitude of household food insecurity was higher compared to the national average, and factors such as the current occupational status of the household head, dependency ratio, and wealth status of the household were significantly associated with household food insecurity. Therefore, policymakers and programmers should provide new strategies focusing on additional income-generating activities and salary increments and consider free services such as school fees and healthcare.
A case report and literature review on tocilizumab-cured acute necrotizing encephalopathy caused by influenza A virus
Authors: Huang YuKun , Zhou Bin , Hong ShaoXian , Cai YaLi
Published: April 2024

Introduction

Acute Necrotizing Encephalopathy (ANE), is a kind of severe Central Nervous System Disease. The commonest pathogen is the influenza virus. The pathogenesis of ANE is bound up to genetic susceptibility and cytokine storm. Interleukin-6 (IL-6) is deemed as the core function in cytokine storm of ANE and that plays a significant role in evaluating the severity of Influenza-Related ANE. Tocilizumab, an IL-6 antagonist, is known to be safe and effective in the treatment of ANE when used early and has an essential role in improving prognosis and preventing disability.

Case report

This case reports a 2 year 10 month old boy who developed ANE after being infected with influenza A virus (H1N1-2019). After treatment with Tocilizumab, the child's consciousness was clear, no convulsions occurred, the movement of limbs was improved, and the lesions of encephalopathy were significantly reduced.

Conclusion

The early use of Tocilizumab is safe and effective for the treatment of ANE caused by influenza virus.
Lipophilic analogues of D-cysteine prevent and reverse physical dependence to fentanyl in male rats
Authors: Bates James N. , Getsy Paulina M. , Coffee Gregory A. , Baby Santhosh M. , MacFarlane Peter M. , Hsieh Yee-Hsee , Knauss Zackery T. , Bubier Jason A. , Mueller Devin , Lewis Stephen J.
Published: April 2024

We examined whether co-injections of the cell-permeant D-cysteine analogues, D-cysteine ethyl ester (D-CYSee) and D-cysteine ethyl amide (D-CYSea), prevent acquisition of physical dependence induced by twice-daily injections of fentanyl, and reverse acquired dependence to these injections in freely-moving male Sprague Dawley rats. Injection of the opioid receptor antagonist, naloxone HCl (NLX, 1.5 mg/kg, IV), elicited a series of withdrawal phenomena that included cardiorespiratory and behavioral responses, and falls in body weight and body temperature, in rats that received 5 or 10 injections of fentanyl (125 μg/kg, IV), and the same number of vehicle co-injections. Regarding the development of physical dependence, the NLX-precipitated withdrawal phenomena were markedly reduced in fentanyl-injected rats that had received co-injections of D-CYSee (250 μmol/kg, IV) or D-CYSea (100 μmol/kg, IV), but not D-cysteine (250 μmol/kg, IV). Regarding reversal of established dependence to fentanyl, the NLX-precipitated withdrawal phenomena in rats that had received 10 injections of fentanyl (125 μg/kg, IV) was markedly reduced in rats that received co-injections of D-CYSee (250 μmol/kg, IV) or D-CYSea (100 μmol/kg, IV), but not D-cysteine (250 μmol/kg, IV), starting with injection 6 of fentanyl. This study provides evidence that co-injections of D-CYSee and D-CYSea prevent the acquisition of physical dependence, and reverse acquired dependence to fentanyl in male rats. The lack of effect of D-cysteine suggests that the enhanced cell-penetrability of D-CYSee and D-CYSea into cells, particularly within the brain, is key to their ability to interact with intracellular signaling events involved in acquisition to physical dependence to fentanyl.
Antimicrobial photodynamic therapy for dermatological infections: current insights and future prospects
Authors: Almenara-Blasco M. , Pérez-Laguna V. , Navarro-Bielsa A. , Gracia-Cazaña T. , Gilaberte Y.
Published: April 2024

Introduction:

Light-based antimicrobial photodynamic therapy (aPDT) shows promise against a wide range of microorganisms, including drug-resistant strains, with low resistance development likelihood. However, its limitations include the need for light exposure, suitability for local infections, and limited industry interest. Relevant studies on aPDT for various infections are discussed.

Methods:

We conducted an extensive literature review on aPDT encompassing articles published from the year 2000 to the current date. To compile comprehensive data, multiple databases (PubMed, Web of Science, Scopus, and Cochrane Library) were systematically searched for relevant studies in both English and Spanish languages. The search strategy involved a combination of Medical Subject Headings (MeSH) terms and free-text terms. The terms included variations and combinations of “Antimicrobial Photodynamic Therapy,” “aPDT,” “photodynamic inactivation,” “microbial photoinactivation,” among others. To ensure the reliability and quality of the information gathered, articles were screened based on relevance to aPDT in antimicrobial therapy, methodological rigor, and alignment with the objectives of this review.

Results:

aPDT shows promise in treating various cutaneous infections. It effectively targets fungal infections, including caused by dermatophytes and Malassezia spp., with notable results against dermatophytosis and tinea capitis. New photosensitizers, like nanoencapsulated hypericin, hold potential for treating these infections. In onychomycosis, aminolevulinic acid-aPDT or methylene blue-aPDT offers an alternative to conventional therapies, showing cure rates as high as 90%. For bacterial infections, it presents a solution against antibiotic-resistant strains and infected ulcers, with potential benefits for wound healing. Regarding viral infections, it indirectly targets viruses by impacting host cells’ metabolism, showing efficacy against human papillomavirus and herpes viruses. Lastly, aPDT demonstrates success in treating parasitic infections, particularly leishmaniasis, with cure rates of up to 100%.

Discussion:

aPDT is increasingly used in dermatological infections, showing efficacy against various microorganisms and stimulating wound healing. It can combat resistant strains and mixed infections, especially in chronic wounds. Combining photodynamic therapy with antimicrobials is a promising strategy to address antimicrobial resistance, but there are practical limitations. Further research is needed for home-based protocols, portable devices, and adapting photosensitizers, as aPDT holds potential as an alternative treatment for recalcitrant infections and immunosuppressed patients, though challenges remain in application.