bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the Palatability of Lamb and Beef Components Used in the Production of Pet Food for Cats
Authors: Watson Pavinee , Thomas David , Hoggard Adrian , Parker Michael , Schreurs Nicola
Published: April 2020

The pet food industry continues to utilise large amounts of inedible meat components from the human food industry. Although used extensively in pet food formulations and as palatants, little is known about the palatability of individual meat components. The objectives of this study were to investigate the palatability of raw meat components commonly used in the production of pet food, using acceptance and preference testing. Those examined were lung, heart, kidney, tripe, liver and mechanically deboned meat (MDM) from lamb and beef. Two-bowl acceptance tests were used to develop an overall ranking of components within each species. Two-bowl preference tests between equivalent beef and lamb components were then used to determine whether a preference was exhibited for one species over the other. For the acceptance of components from lamb and beef, liver was the most palatable within both species, with kidney equivalent to liver when testing lamb components. The MDM was identified as the least palatable component from both species. When examining the preference between equivalent components between species, cats showed preferences for lamb over equivalent beef components, except for heart and liver which showed no difference in intake between the two species. Overall, cats were able to clearly rank the palatability of different components from lamb and beef, as well as between equivalent components from the two species. Selecting highly palatable ingredients whilst still meeting pet food manufacturing guidelines may play a role in improving overall diet palatability and acceptance by cats.
Effects of Increasing Doses of Lactobacillus Pre-Fermented Rapeseed Product with or without Inclusion of Macroalgae Product on Weaner Piglet Performance and Intestinal Development
Authors: Satessa Gizaw Dabessa , Tamez-Hidalgo Paulina , Kjærulff Søren , Vargas-Bello-Pérez Einar , Dhakal Rajan , Nielsen Mette Olaf
Published: April 2020

This study evaluated the effects of increasing doses of pre-fermented rapeseed meal (FRM) without or with inclusion of the brown macroalgae Ascophyllum nodosum (AN) on weaner piglets’ performance and gut development. Ten days pre-weaning, standardized litters were randomly assigned to one of nine isoenergetic and isoproteic diets comprising (on DM basis): no supplement (negative control, NC), 2500 ppm ZnO (positive control, PC), 8, 10, 12, 15 or 25% FRM, and 10% FRM plus 0.6 or 1.0% AN. Fifty piglets receiving the same pre-weaning diets were weaned at 28 days of age and transferred to one pen, where they continued on the pre-weaning diet until day 92. At 41 days, six piglets per treatment were sacrificed for blood and intestinal samplings. The average daily gain was at least sustained at any dose of FRM (increased at 8% FRM, 28–41 days) from 18–41 days similar to PC but unaffected by inclusion of AN. The percentage of piglets that completed the experiment was increased by FRM compared to NC, despite detection of diarrhea symptoms. FRM showed quadratic dose-response effects on colon and mid-jejunum crypts depth, and enterocyte and mid-jejunum villus heights with optimum development at 8% or 10% FRM, respectively, but this was abolished when AN was also added. In conclusion, FRM sustained piglet growth performance and intestinal development similar to ZnO with an optimum inclusion level of 8–10% of dietary DM.
Low Expression of Sirtuin 1 in the Dairy Cows with Mild Fatty Liver Alters Hepatic Lipid Metabolism
Authors: Li Yu , Zou Suping , Ding Hongyan , Hao Ning , Huang Yingying , Tang Jishun , Cheng Jianbo , Feng Shibin , Li Jinchun , Wang Xichun , Wu Jinjie
Published: April 2020

Dairy cows usually experience negative energy balance coupled with an increased incidence of fatty liver during the periparturient period. The purpose of this study was to investigate the effect of hepatic steatosis on the expression of the sirtuin 1 (SIRT1), along with the target mRNA and protein expressions and activities related to lipid metabolism in liver tissue. Control cows (n = 6, parity 3.0 ± 2.0, milk production 28 ± 7 kg/d) and mild fatty liver cows (n = 6, parity 2.3 ± 1.5, milk production 20 ± 6 kg/d) were retrospectively selected based on liver triglycerides (TG) content (% wet liver). Compared with the control group, fatty liver cows had greater concentrations of cholesterol and TG along with the typically vacuolated appearance and greater lipid droplets in the liver. Furthermore, fatty liver cows had greater mRNA and protein abundance related to hepatic lipid synthesis proteins sterol regulatory element binding proteins (SREBP-1c), long-chain acyl-CoA synthetase (ACSL), acyl-CoA carbrolase (ACC) and fatty acid synthase (FAS) and lipid transport proteins Liver fatty acid binding protein (L-FABP), apolipoprotein E (ApoE), low density lipoprotein receptor (LDLR) and microsomal TG transfer protein (MTTP) (p < 0.05). However, they had lower mRNA and protein abundance associated with fatty acid β-oxidation proteins SIRT1, peroxisome proliferator-activated receptor co-activator-1 (PGC-1α), peroxisome proliferator–activated receptor-α (PPARα), retinoid X receptor (RXRα), acyl-CoA 1 (ACO), carnitine palmitoyltransferase 1 (CPT1), carnitine palmitoyltransferase 2 (CPT2) and long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases (LCAD) (p < 0.05). Additionally, mRNA abundance and enzyme activity of enzymes copper/zinc superoxide dismutase (Cu/Zn SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and manganese superoxide dismutase (Mn SOD) decreased and mRNA and protein abundance of p45 nuclear factor-erythroid 2 (p45 NF-E2)-related factor 1 (Nrf1), mitochondrial transcription factor A (TFAM) decreased (p < 0.05). Lower enzyme activities of SIRT1, PGC-1α, Cu/Zn SOD, CAT, GSH-Px, SREBP-1c and Mn SOD (p < 0.05) and concentration of reactive oxygen species (ROS) were observed in dairy cows with fatty liver. These results demonstrate that decreased SIRT1 associated with hepatic steatosis promotes hepatic fatty acid synthesis and inhibits fatty acid β-oxidation. Hence, SIRT1 may represent a novel therapeutic target for the treatment of the fatty liver disease in dairy cows.
Effects of Single Nucleotide Polymorphisms in the SLC27A3 Gene on the Nutritional Value of Sheep Milk
Authors: Pecka-Kiełb Ewa , Kowalewska-Łuczak Inga , Czerniawska-Piątkowska Ewa , Zielak-Steciwko Anna E.
Published: April 2020

The current research was undertaken to use the genetic potential of animals to obtain high-quality dairy products. Single nucleotide polymorphisms (SNPs) in SLC27A3 gene were identified in Zošľachtená valaška sheep using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Correlations between genotypes and milk composition and nutritional value were analysed This study showed that milk from sheep with TT genotype in the SNP4 locus was characterised by higher (p < 0.01) fat and dry matter content and lower lactose concentration, compared to sheep with AA and TA genotypes, respectively. Moreover, it was found that animals with GG genotype in SNP1 produced milk with higher C18:1n9c, C18:1n7t, CLA, and other unsaturated fatty acids (UFAs) content than sheep with TT. Additionally, milk from animals with CC at the SNP3 locus had significantly higher (p < 0.01) levels of UFAs than milk from sheep with other genotypes in the SNP3. In summary, it may be concluded that milk from animals with TT genotype of SNP4 is characterised by higher fat and dry matter content. Whereas, milk from sheep with GG in SNP1 and with CC in SNP3 is characterised by higher content of UFAs, which increases milk value as material for functional food production.
Copy Number Variations and Expression Levels of Guanylate-Binding Protein 6 Gene Associated with Growth Traits of Chinese Cattle
Authors: Hao Dan , Wang Xiao , Thomsen Bo , Kadarmideen Haja N. , Wang Xiaogang , Lan Xianyong , Huang Yongzhen , Qi Xinglei , Chen Hong
Published: April 2020

Association studies have indicated profound effects of copy number variations (CNVs) on various phenotypes in different species. In this study, we identified the CNV distributions and expression levels of guanylate-binding protein 6 (GBP6) associated with the growth traits of Chinese cattle. The results showed that the phenotypic values of body size and weight of Xianan (XN) cattle were higher than those of Nanyang (NY) cattle. The medium CNV types were mostly identified in the XN and NY breeds, but their CNV distributions were significantly different (adjusted p < 0.05). The association analysis revealed that the body weight, cannon circumference and chest circumference of XN cattle had significantly different values in different CNV types (p < 0.05), with CNV gain types (Log22−ΔΔCt > 0.5) displaying superior phenotypic values. We also found that transcription levels varied in different tissues (p < 0.001) and the CNV gain types showed the highest relative gene expression levels in the muscle tissue, consistent with the highest phenotypic values of body weight and cannon circumference among the three CNV types. Consequently, our results suggested that CNV gain types of GBP6 could be used as the candidate markers in the cattle-breeding program for growth traits.
Flexible Foraging Response of Wintering Hooded Cranes (Grus monacha) to Food Availability in the Lakes of the Yangtze River Floodplain, China
Authors: Wei Zhenhua , Zheng Meng , Zhou Lizhi , Xu Wenbin
Published: April 2020

Wetlands are disappearing or degrading at an unprecedented rate due to the increase in human encroachment and disturbance, eventually leading to habitat loss for waterbirds, which is the primary cause of the decline in the Hooded Crane (Grus monacha) population. The Hooded Cranes have to constantly adjust their foraging strategies to survive to cope with this situation. In order to study how cranes respond to food resources in mosaic habitat, we surveyed a total of 420 food quadrats and 736 behavioral samples from three habitats during three wintering periods in Shengjin Lake and Caizi Lake. We measured temporal and between-habitat differences in foraging time budget, foraging frequency, and foraging success rate. Akaike’s information criterion was selected between the models of food abundance and availability. The results indicated that the wintering cranes spent the majority of their time (66.55%) foraging and shifted their foraging behaviors based upon food abundance and availability in different habitats. Our analyses also indicated that cranes were willing to forage more food with poor sediment penetrability in sub-optimal habitats. Foraging time budget was based on the food depth, and the foraging frequency and foraging success rate were based on food abundance. Cranes adopted flexible foraging strategies in response to the alternative food resources in mosaic wetland habitats, as it could mitigate the negative impacts of habitat loss and facilitate survival.
The Effect of Integrating Genomic Information into Genetic Evaluations of Chinese Merino Sheep
Authors: Wei Chen , Luo Hanpeng , Zhao Bingru , Tian Kechuan , Huang Xixia , Wang Yachun , Fu Xuefeng , Tian Yuezhen , Di Jiang , Xu Xinming , Wu Weiwei , Tulafu Hanikezi , Yasen Maerziya , Zhang Yajun , Zhao Wensheng
Published: April 2020

Genomic evaluations are a method for improving the accuracy of breeding value estimation. This study aimed to compare estimates of genetic parameters and the accuracy of breeding values for wool traits in Merino sheep between pedigree-based best linear unbiased prediction (PBLUP) and single-step genomic best linear unbiased prediction (ssGBLUP) using Bayesian inference. Data were collected from 28,391 yearlings of Chinese Merino sheep (classified in 1992–2018) at the Xinjiang Gonaisi Fine Wool Sheep-Breeding Farm, China. Subjectively-assessed wool traits, namely, spinning count (SC), crimp definition (CRIM), oil (OIL), and body size (BS), and objectively-measured traits, namely, fleece length (FL), greasy fleece weight (GFW), mean fiber diameter (MFD), crimp number (CN), and body weight pre-shearing (BWPS), were analyzed. The estimates of heritability for wool traits were low to moderate. The largest h2 values were observed for FL (0.277) and MFD (0.290) with ssGBLUP. The heritabilities estimated for wool traits with ssGBLUP were slightly higher than those obtained with PBLUP. The accuracies of breeding values were low to moderate, ranging from 0.362 to 0.573 for the whole population and from 0.318 to 0.676 for the genotyped subpopulation. The correlation between the estimated breeding values (EBVs) and genomic EBVs (GEBVs) ranged from 0.717 to 0.862 for the whole population, and the relative increase in accuracy when comparing EBVs with GEBVs ranged from 0.372% to 7.486% for these traits. However, in the genotyped population, the rank correlation between the estimates obtained with PBLUP and ssGBLUP was reduced to 0.525 to 0.769, with increases in average accuracy of 3.016% to 11.736% for the GEBVs in relation to the EBVs. Thus, genomic information could allow us to more accurately estimate the relationships between animals and improve estimates of heritability and the accuracy of breeding values by ssGBLUP.
Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders
Authors: Akhtar Muhammad Faheem , Ahmad Ejaz , Mustafa Sheeraz , Chen Zhe , Shi Zhendan , Shi Fangxiong
Published: April 2020

The past three decades revolutionized the goose industry in the world. China holds the world’s largest goose breeds stock by 95% of the global total. To optimize the goose industry and cope with ever increasing poultry meat and egg demands, there is a dire need to focus on reproduction, as most geese breeds exhibit poor reproductive performance. The present study was conducted with the aim to add a contribution in the goose industry and research by the histological visualizing step wise development of germ cells during spermatogenesis by microscopy and a histological technique. Yangzhou goose is a synthetic breed developed by using local goose germplasm resources of China. It is popular in the Chinese goose industry due to high productivity and adaptability. This research evaluated the steps of spermiogenesis and stages along with morphological changes in the seminiferous epithelium in Yangzhou goose ganders. For the assessment of various stages of the seminiferous epithelium cycle, testis sections were embedded in molten paraffin wax. The initial steps of spermiogenesis were depicted by changes in acrosomic granules, whereas further stages were identified by nuclear morphological changes. Ten steps of spermiogenesis and nine stages of seminiferous epithelium were identified. Four types of spermatogonia Ad, Ap1, Ap2 and B were recognized. The results depicted a clear variation in the diameter of seminiferous tubules (ST), epithelium height (EH), luminal tubular diameter (LD), number of seminiferous tubules per field and the Johnsen score. Microscopy indicated that the stages of seminiferous epithelium were similar to other birds and mammals and the ST diameter, EH, LD and Johnsen score are positively correlated while the number of seminiferous tubules per field is negatively correlated with the ST diameter, EH, LD and Johnsen score. Fertility in Yangzhou ganders can further be improved by visualizing the histological development of germs cells in testis tissues during spermatogenesis after onset of breeding season and maturity. Our results suggest that Yangzhou ganders reach complete sexual maturity at 227 days of age.
Mouth Pain in Horses: Physiological Foundations, Behavioural Indices, Welfare Implications, and a Suggested Solution
Authors: Mellor David J.
Published: April 2020

A proposition addressed here is that, although bitted horses are viewed by many equestrians as being largely free of bit-related mouth pain, it seems likely that most behavioural signs of such pain are simply not recognised. Background information is provided on the following: the major features of pain generation and experience; cerebrocortical involvement in the conscious experience of pain by mammals; the numerous other subjective experiences mammals can have; adjunct physiological responses to pain; some general feature of behavioural responses to pain; and the neural bases of sensations generated within the mouth. Mouth pain in horses is then discussed. The areas considered exclude dental disease, but they include the stimulation of pain receptors by bits in the interdental space, the tongue, the commissures of the mouth, and the buccal mucosa. Compression, laceration, inflammation, impeded tissue blood flow, and tissue stretching are evaluated as noxious stimuli. The high pain sensitivity of the interdental space is described, as are likely increases in pain sensitivity due to repeated bit contact with bruises, cuts, tears, and/or ulcers wherever they are located in the mouth. Behavioural indices of mouth pain are then identified by contrasting the behaviours of horses when wearing bitted bridles, when changed from bitted to bit-free bridles, and when free-roaming unbitted in the wild. Observed indicative behaviours involve mouth movements, head-neck position, and facial expression (“pain face”), as well as characteristic body movements and gait. The welfare impacts of bit-related pain include the noxiousness of the pain itself as well as likely anxiety when anticipating the pain and fear whilst experiencing it, especially if the pain is severe. In addition, particular mouth behaviours impede airflow within the air passages of the upper respiratory system, effects that, in their turn, adversely affect the air passages in the lungs. Here, they increase airflow resistance and decrease alveolar gas exchange, giving rise to suffocating experiences of breathlessness. In addition, breathlessness is a likely consequence of the low jowl angles commonly maintained during dressage. If severe, as with pain, the prospect of breathlessness is likely to give rise to anxiety and the direct experience of breathlessness to fear. The related components of welfare compromise therefore likely involve pain, breathlessness, anxiety, and fear. Finally, a 12-point strategy is proposed to give greater impetus to a wider adoption of bit-free bridles in order to avoid bit-induced mouth pain.
Analysis of Astragalus Polysaccharide Intervention in Heat-Stressed Dairy Cows’ Serum Metabolomics
Authors: Zeng Hanfang , Xi Yumeng , Li Yeqing , Wang Zedong , Zhang Lin , Han Zhaoyu
Published: April 2020

This experiment was conducted to investigate the effects of astragalus polysaccharides (APS) on serum metabolism of dairy cows under heat stress. Thirty healthy Holstein dairy cows were randomly divided into three groups (10 cows in each group). In the experimental group, 30 mL/d (Treatment I) and 50 mL/d (Treatment II) of APS injection were injected into the neck muscle respectively. Each stage was injected with APS for 4 days (8:00 a.m. every day) and stopped for 3 days. Serum hormone and antioxidant indexes of dairy cows were investigated. Through repeated measurement analysis of variance, the results have shown that cortisol (COR) (F = 6.982, p = 0.026), triiodothyronine (T3) (F = 10.005, p = 0.012) and thyroxine (T4) (F = 22.530, p = 0.002) at different time points were significantly different. COR showed a downward trend, T3 and T4 showed an upward trend. At each time point, different concentrations of APS have significant effects on COR (F = 30.298, p = 0.000 < 0.05), T3 (F = 18.122, p = 0.001), and T4 (F = 44.067, p = 0.000 < 0.05). However, there were no significant differences in serum insulin (INS), glucagon (GC) and heat shock protein 70 (HSP70) between different time points (p > 0.05) and at each time point (p > 0.05). Additionally, the results have also shown that there were also no significant differences in serum Superoxide dismutase (SOD), malondialdehyde (MDA) and lactate dehydrogenase (LDH) between different time points (p > 0.05) and at each time point (p > 0.05). However, the injection of APS had a significant impact on glutathione peroxidase (GSH-Px) (F = 9.421, p = 0.014) at different times, and showed a trend of rising first and then falling. At each time point, APS of different concentrations had no significant effect on GSH-Px (p > 0.05). Furthermore, we used gas chromatography–mass spectrometry (GC-MS) non-targeted metabolomics to determine the potential markers of APS for heat-stressed dairy cows. Twenty metabolites were identified as potential biomarkers for the diagnosis of APS in heat-stressed dairy cows. These substances are involved in protein digestion and absorption, glutathione metabolism, prolactin signaling pathway, aminoacyl-tRNA biosynthesis, pentose and glucuronate interconversions, and so on. Our findings suggest that APS have an effect on the serum hormones of heat-stressed dairy cows, and regulate the metabolism of heat-stressed dairy cows through glucose metabolism and amino acid metabolism pathways.
Improvement of Cecal Commensal Microbiome Following the Insect Additive into Chicken Diet
Authors: Józefiak Agata , Benzertiha Abdelbasset , Kierończyk Bartosz , Łukomska Anna , Wesołowska Izabela , Rawski Mateusz
Published: April 2020

Gastrointestinal microbiota play an important role in regulating the metabolic processes of animals and humans. A properly balanced cecal microbiota modulates growth parameters and the risk of infections. The study examined the effect of the addition of 0.2% and 0.3% of Tenebrio molitor and Zophobas morio on cecal microbiome of broilers. The material was the cecum digesta. The obtained DNA was analyzed using 16S rRNA next generation sequencing. The results of the study show that the addition of a relatively small amount of Z. morio and T. molitor modulates the broiler cecum microbiome composition. The most positive effect on cecal microbiota was recorded in the 0.2% Z. morio diet. A significant increase in the relative amount of genus Lactobacillus, represented by the species Lactobacillus agilis and the amount of bacteria in the Clostridia class, was observed. Moreover, the addition of 0.2% ZM resulted in a significant increase of relative abundance of the family Bifidobacteriaceae with the highest relative abundance of genus Bifidobacterium pseudolongum. The obtained results indicate that the addition of a relatively small amount of insect meal in broiler diet stimulates colonization by probiotic and commensal bacteria, which may act as barriers against infection by pathogenic bacteria.
Long-Term Socialization with Humans Affects Human-Directed Behavior in Goats
Authors: Mastellone Vincenzo , Scandurra Anna , D’Aniello Biagio , Nawroth Christian , Saggese Fiorella , Silvestre Pasqualino , Lombardi Pietro
Published: April 2020

Throughout their evolutionary history, humans have tried to domesticate a variety of wild terrestrial mammals, resulting in a limited number that has been successfully domesticated. Among these domesticated species, domestic goats (Capra aegagrus hircus) are a useful model species to study the effects of ontogenesis on the socio-cognitive abilities of domestic non-companion animals in their interactions with humans. To this end, the behavioral responses of two groups of goats with a different background of human socialization (high and low socialization) were compared in the impossible task test, an experimental paradigm aimed to study socio-cognitive skills and the tendency to interact with humans. Our results show that, when the task became impossible to solve, goats with a higher level of socialization interacted with the experimenter for a greater amount of time than subjects in the low socialization group, whereas the latter group exhibited increased door directed behavior. Overall, highly socialized goats made more social contact with humans compared to the other group in the impossible task paradigm.
Identification of Ancestry Informative Marker (AIM) Panels to Assess Hybridisation between Feral and Domestic Sheep
Authors: Somenzi Elisa , Ajmone-Marsan Paolo , Barbato Mario
Published: April 2020

Hybridisation of wild populations with their domestic counterparts can lead to the loss of wildtype genetic integrity, outbreeding depression, and loss of adaptive features. The Mediterranean island of Sardinia hosts one of the last extant autochthonous European mouflon (Ovis aries musimon) populations. Although conservation policies, including reintroduction plans, have been enforced to preserve Sardinian mouflon, crossbreeding with domestic sheep has been documented. We identified panels of single nucleotide polymorphisms (SNPs) that could act as ancestry informative markers able to assess admixture in feral x domestic sheep hybrids. The medium-density SNP array genotyping data of Sardinian mouflon and domestic sheep (O. aries aries) showing pure ancestry were used as references. We applied a two-step selection algorithm to this data consisting of preselection via Principal Component Analysis followed by a supervised machine learning classification method based on random forest to develop SNP panels of various sizes. We generated ancestry informative marker (AIM) panels and tested their ability to assess admixture in mouflon x domestic sheep hybrids both in simulated and real populations of known ancestry proportions. All the AIM panels recorded high correlations with the ancestry proportion computed using the full medium-density SNP array. The AIM panels proposed here may be used by conservation practitioners as diagnostic tools to exclude hybrids from reintroduction plans and improve conservation strategies for mouflon populations.
Salmonella in Free-Ranging Quokkas (Setonix brachyurus) from Rottnest Island and the Mainland of Western Australia
Authors: Martínez-Pérez Pedro , Hyndman Timothy H. , Fleming Patricia A.
Published: April 2020

Salmonella is a genus of Gram-negative, motile, and facultative anaerobic bacteria with a worldwide distribution that contaminates multiple substrates (vegetation, food, soil, and water) and inhabits the gastrointestinal tract of birds, reptiles, and mammals, including humans. Rottnest Island is a popular tourist destination and is abundantly inhabited by quokkas (Setonix brachyurus), a charismatic small wallaby. Current data on the association between Salmonella and quokkas on Rottnest Island are outdated by approximately 30 years. Additionally, previous studies on quokkas on this island and mainland Western Australia did not perform physical examinations or any diagnostic tests. The aim of the project was to assess the prevalence of Salmonella spp. in quokkas from Rottnest Island and mainland Western Australia and correlate the presence of the bacterium with the health of the animal. Ninety-two quokkas from Rottnest Island (n = 71) and populations on the mainland (n = 21) were screened for Salmonella, and a prevalence of 47.9% and 4.8%, respectively, was determined. A total of 16 serovars were identified from 37 isolates; five of these serovars had previously not been described in the quokka. Salmonella appeared to have an effect on the haematology and blood chemistry of quokkas on Rottnest Island consistent with subclinical salmonellosis. The health of Rottnest Island quokkas, and their potential impact on the health of the visitors to the island, should continue to be monitored carefully.
Welfare Challenges of Dairy Cows in India Identified Through On-Farm Observations
Authors: Mullan Siobhan , Bunglavan Surej J. , Rowe Elizabeth , Barrett David C. , Lee Michael R. F. , Ananth Deepa , Tarlton John
Published: April 2020

India has the largest population of dairy cattle in the world at over 48 million animals, yet there has been little formal assessment of their welfare reported. Through observations of dairy cows on 38 farms in Kerala, India, we aimed to investigate the welfare of these animals and the practicality of animal-based assessments within common farming systems. Substantial welfare challenges were identified. All cows were close-tied (less than 1 m length) via a halter that pierced the nasal septum when housed, which was for the entire day (50% of farms) or part thereof. When outside access was available, it was also usually restricted by close-tying, longline tether, or hobbling. Ad libitum water was only available on 22% of farms and food access was also restricted (mean of 4.3 h/day). Future work should focus on encouraging dairy farmers in India to improve the welfare of their dairy cattle by: ceasing to tie and tether cattle (or at least providing tied and tethered cattle with exercise opportunities); providing unlimited access to drinking water and a readier supply of food (especially quality green forage/fodder); cleaning housing more frequently; providing strategies to prevent heat stress; breeding cattle suited to environmental conditions and with increased resistance to heat stress; and carrying out welfare assessments more regularly using a validated protocol and rectifying the causes of poor welfare. Such changes could substantially improve the welfare of tens of millions of cattle.
High Salt Diet Affects the Reproductive Health in Animals: An Overview
Authors: Abdelnour Sameh A. , Abd El-Hack Mohamed E. , Noreldin Ahmed E. , Batiha Gaber Elsaber , Beshbishy Amani Magdy , Ohran Husein , Khafaga Asmaa F. , Othman Sarah I. , Allam Ahmed A. , Swelum Ayman A.
Published: April 2020

Salinity is a reliable issue of crop productivity loss in the world and in certain tropical and subtropical zones. However, tremendous progress in the genetic improvement of plants for salinity tolerance has been made over several decades. In light of this, halophytic plants can be used as animal feeds and have promising features because they are a good feed resource. However, the main constraint of saline pasture systems is the extreme concentration of NaCl salt in drinking water and forage plants for grazing animals. Ecological reports revealed that excess diet salt causes mortality and morbidity worldwide. Animal fed halophytic forages may have adverse effects on growth performance and reproductive function in males and females due to inducing reductions in hormone regulation, such as testosterone, FSH, LH, and leptin. It was indicated that high salt intake promotes circulating inflammatory factors in the placenta and is associated with adversative effects on pregnancy. This review focuses on the scientific evidence related to the effect of high salt intake on growth performance, spermatogenesis, sperm function, and testicular morphology changes in male animals. In addition, the review will also focus on its effect on some female reproductive features (e.g., ovarian follicle developments, placental indices, and granulosa cell function).
Exogenous Oleic Acid and Palmitic Acid Improve Boar Sperm Motility via Enhancing Mitochondrial Β-Oxidation for ATP Generation
Authors: Zhu Zhendong , Li Rongnan , Feng Chengwen , Liu Ruifang , Zheng Yi , Hoque S. A. Masudul , Wu De , Lu Hongzhao , Zhang Tao , Zeng Wenxian
Published: April 2020

It takes several hours for mammalian sperm to migrate from the ejaculation or insemination site to the fertilization site in the female reproductive tract in which glucose, amino acids, and fatty acids are regarded as the primary substrates for ATP generation. The present study was designed to investigate whether oleic acid and palmitic acid were beneficial to boar sperm in vitro; and if yes, to elucidate the mechanism that regulates sperm motility. Therefore, the levels of oleic acid and palmitic acid, motility, membrane integrity, acrosome integrity, and apoptosis of sperm were evaluated. Moreover, the enzymes involved in mitochondrial β-oxidation (CPT1: carnitine palmitoyltransferase 1; ACADVL: long-chain acyl-coenzyme A dehydrogenase) were detected with immunofluorescence and Western blotting. Consequently, the ATP content and the activities of CPT1, ACADVL, malate dehydrogenase (MDH), and succinate dehydrogenase (SDH) were also measured. We observed that CPT1 and ACADVL were expressed in boar sperm and localized in the midpiece. The levels of oleic acid and palmitic acid were decreased during storage at 17 °C. The addition of oleic acid and palmitic acid significantly increased sperm motility, progressive motility, straight-line velocity (VSL), membrane integrity, and acrosome integrity with a simultaneous decrease in sperm apoptosis after seven days during storage. When sperm were incubated with oleic acid and palmitic acid at 37 °C for 3 h, the activities of CPT1 and ACADVL, the ATP level, the mitochondrial membrane potential, the activities of MDH and SDH, as well as sperm motility patterns were significantly increased compared to the control (p < 0.05). Moreover, the addition of etomoxir to the diluted medium in the presence of either oleic acid or palmitic acid and the positive effects of oleic acid and palmitic acid were counteracted. Together, these data suggest that boar sperm might utilize oleic acid and palmitic acid as energy substrates for ATP production via β-oxidation. The addition of these acids could improve sperm quality.
Injection of Raffinose Family Oligosaccharides at 12 Days of Egg Incubation Modulates the Gut Development and Resistance to Opportunistic Pathogens in Broiler Chickens
Authors: Stadnicka Katarzyna , Bogucka Joanna , Stanek Magdalena , Graczyk Radomir , Krajewski Krzysztof , Maiorano Giuseppe , Bednarczyk Marek
Published: April 2020

The aim was to investigate the impact of an automatic in ovo injection of the raffinose family oligosaccharides (RFO) extracted from the seeds of Lupinus luteus L, on the chicken performance and resistance in a production environment. At day 12 of incubation, a total of 57,900 eggs (Ross 308) were divided into two groups: 1/ Control, injected with 0.9% NaCl and 2/ RFO group, injected with 1.9 mg/egg of the lupin seed extract, dissolved in 0.2 mL NaCl. The performance parameters, biochemical indices (lipid profile, hepatic parameters), gut histomorphology and duodenum structure, oxidative stability of the meat and microbiological counts of the major commensal microbiota species were analyzed. Mortality, body weight, and feed conversion ratio (FCR) were not affected. By day 42, several health indices were improved with RFO and were reflected in a beneficial lipid blood profile, increased villi surface and better combating opportunistic pathogens through reduction of Clostridia and decreased coccidia counts. The RFO increased meat oxidation, but only at the beginning of the storage. The RFO sourced from local legumes can be considered a promising prebiotic for broiler chickens. In ovo delivery of prebiotics and/or synbiotics should be further optimized as an important strategy for the earliest possible modulation of chicken resistance.
Separate and Synergic Effects of Lactobacillus uvarum LUHSS245 and Arabinogalactan on the In Vitro Antimicrobial Properties as Well as on the Fecal and Metabolic Profile of Newborn Calves
Authors: Zavistanaviciute Paulina , Lele Vita , Antanaitis Ramūnas , Televičius Mindaugas , Ruzauskas Modestas , Zebeli Qendrim , Bartkiene Elena
Published: April 2020

In this study, arabinogalactan (ARB) and Lactobacillus uvarum LUHS245 antimicrobial properties against pathogenic bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa 17-331, Acinetobacter baumanni 17-380, Proteus mirabilis, MRSA M87fox, Enterococcus faecalis 86, Enterococcus faecium 103, Bacillus cereus 18 01, and Streptococcus mutans) and resistance to antibiotics were evaluated and the role of their supplementation on the main metabolic and fecal variables of newborn calves was established. The animal trial involved 48 Holstein female calves randomly allocated in four homogeneous groups of 12 animals each, on the basis of body weight in the second day of life. Calves were fed with a standard milk replacer diet from the second day of life until 14th day, either unsupplemented or supplemented with 50 mL of LUHS245 (≥7.5 log10 CFU mL−1), 30 g of ARB, or with both (50 mL of LUHS245 and 30 g ARB). In vitro data showed that the LUHS245 inhibited the growth of Salmonella enterica and Bacillus cereus (inhibition zones 13.0 and 21.3 mm, respectively). Supplementation of LUHS245 and ARB either alone or together, lowered total bacterial count in the feces and reduced lactate and serum alanine aminotransferase concentrations in blood. This study showed that LUHS245 supplementation alone or together with ARB seemed to have some positive influence on certain health parameters in newborn calves. Further research with larger cohorts of animals is warranted to validate the beneficial effects of the tested supplements.
Monoclonal Antibodies as an Antibacterial Approach Against Bacterial Pathogens
Authors: Zurawski Daniel V. , McLendon Molly K.
Published: April 2020

In the beginning of the 21st century, the frequency of antimicrobial resistance (AMR) has reached an apex, where even 4th and 5th generation antibiotics are becoming useless in clinical settings. In turn, patients are suffering from once-curable infections, with increases in morbidity and mortality. The root cause of many of these infections are the ESKAPEE pathogens (Enterococcus species, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species, and Escherichia coli), which thrive in the nosocomial environment and are the bacterial species that have seen the largest rise in the acquisition of antibiotic resistance genes. While traditional small-molecule development still dominates the antibacterial landscape for solutions to AMR, some researchers are now turning to biological approaches as potential game changers. Monoclonal antibodies (mAbs)—more specifically, human monoclonal antibodies (Hu-mAbs)—have been highly pursued in the anti-cancer, autoimmune, and antiviral fields with many success stories, but antibody development for bacterial infection is still just scratching the surface. The untapped potential for Hu-mAbs to be used as a prophylactic or therapeutic treatment for bacterial infection is exciting, as these biologics do not have the same toxicity hurdles of small molecules, could have less resistance as they often target virulence proteins rather than proteins required for survival, and are narrow spectrum (targeting just one pathogenic species), therefore avoiding the disruption of the microbiome. This mini-review will highlight the current antibacterial mAbs approved for patient use, the success stories for mAb development, and new Hu-mAb products in the antibacterial pipeline.
A Heuristic Solution Method for Multi-Depot Vehicle Routing-Based Waste Collection Problems
Authors: Shi Yanjun , Lv Lingling , Hu Fanyi , Han Qiaomei
Published: April 2020

This paper addresses waste collection problems in which urban household and solid waste are brought from waste collection points to waste disposal plants. The collection of waste from the collection points herein is modeled as a multi-depot vehicle routing problem (MDVRP), aiming at minimizing the total transportation distance. In this study, we propose a heuristic solution method to address this problem. In this method, we firstly assign waste collection points to waste disposal plants according to the nearest distance, then each plant solves the single-vehicle routing problem (VRP) respectively, assigning customers to vehicles and planning the order in which customers are visited by vehicles. In the latter step, we propose the sector combination optimization (SCO) algorithm to generate multiple initial solutions, and then these initial solutions are improved using the merge-head and drop-tail (MHDT) strategy. After a certain number of iterations, the optimal solution in the last generation is reported. Computational experiments on benchmark instances showed that the initial solutions obtained by the sector combination optimization algorithm were more abundant and better than other iterative algorithms using only one solution for initialization, and the solutions with distance gap were obtained using the merge-head and drop-tail strategy in a lower CPU time compared to the Tabu search algorithm.
Learning a Path from Real Navigation: The Advantage of Initial View, Cardinal North and Visuo-Spatial Ability
Authors: Muffato Veronica , Meneghetti Chiara
Published: April 2020

Background: Spatial cognition research strives to maximize conditions favoring environment representation. This study examined how initial (egocentric) navigation headings interact with allocentric references in terms of world-based information (such as cardinal points) in forming environment representations. The role of individual visuo-spatial factors was also examined. Method: Ninety-one undergraduates took an unfamiliar path in two learning conditions, 46 walked from cardinal south to north (SN learning), and 45 walked from cardinal north to south (NS learning). Path recall was tested with SN and NS pointing tasks. Perspective-taking ability and self-reported sense of direction were also assessed. Results: Linear models showed a better performance for SN learning and SN pointing than for NS learning and NS pointing. The learning condition x pointing interaction proved SN pointing more accurate than NS pointing after SN learning, while SN and NS pointing accuracy was similar after NS learning. Perspective-taking ability supported pointing accuracy. Conclusions: These results indicate that initial heading aligned with cardinal north prompt a north-oriented representation. No clear orientation of the representation emerges when the initial heading is aligned with cardinal south. Environment representations are supported by individual perspective-taking ability. These findings offer new insight on the environmental and individual factors facilitating environment representations acquired from navigation.
Targeted Therapies: Friends or Foes for Patient’s NK Cell-Mediated Tumor Immune-Surveillance?
Authors: Damele Laura , Ottonello Selene , Mingari Maria Cristina , Pietra Gabriella , Vitale Chiara
Published: April 2020

In the last 20 years there has been a huge increase in the number of novel drugs for cancer treatment. Most of them exploit their ability to target specific oncogenic mutations in the tumors (targeted therapies–TT), while others target the immune-checkpoint inhibitor molecules (ICI) or the epigenetic DNA modifications. Among them, TT are the longest established drugs exploited against a wide spectrum of both solid and hematological tumors, often with reasonable costs and good efficacy as compared to other innovative therapies (i.e., ICI). Although they have greatly improved the treatment of cancer patients and their survival, patients often relapse or develop drug-resistance, leading to the impossibility to eradicate the disease. The outcome of TT has been often correlated with their ability to affect not only tumor cells, but also the repertoire of immune cells and their ability to interact with cancer cells. Thus, the possibility to create novel synergies among drugs an immunotherapy prompted scientists and physicians to deeply characterize the effects of TT on immune cells both by in-vitro and by ex-vivo analyses. In this context, NK cells may represent a key issue, since they have been shown to exert a potent anti-tumor activity, both against hematological malignancies and solid tumors. In the present review we will discuss most recent ex-vivo analyses that clarify the effect of TT treatment on patient’s NK cells comparing them with clinical outcome and previous in-vitro data.
Recent Advances in Immunotherapy for Hepatocellular Carcinoma
Authors: Nakano Shigeharu , Eso Yuji , Okada Hirokazu , Takai Atsushi , Takahashi Ken , Seno Hiroshi
Published: April 2020

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death since most patients are diagnosed at advanced stage and the current systemic treatment options using molecular-targeted drugs remain unsatisfactory. However, the recent success of cancer immunotherapies has revolutionized the landscape of cancer therapy. Since HCC is characterized by metachronous multicentric occurrence, immunotherapies that induce systemic and durable responses could be an appealing treatment option. Despite the suppressive milieu of the liver and tumor immunosurveillance escape mechanisms, clinical studies of checkpoint inhibitors in patients with advanced HCC have yielded promising results. Here, we provide an update on recent advances in HCC immunotherapies. First, we describe the unique tolerogenic properties of hepatic immunity and its interaction with HCC and then review the status of already or nearly available immune checkpoint blockade-based therapies as well as other immunotherapy strategies at the preclinical or clinical trial stage.
Biobanking in Molecular Biomarker Research for the Early Detection of Cancer
Authors: Lommen Kim , Odeh Selena , de Theije Chiel C. , Smits Kim M.
Published: April 2020

Although population-wide screening programs for several cancer types have been implemented in multiple countries, screening procedures are invasive, time-consuming and often perceived as a burden for patients. Molecular biomarkers measurable in non-invasively collected samples (liquid biopsies) could facilitate screening, as they could have incremental value on early diagnosis of cancer, but could also predict prognosis or monitor treatment response. Although the shift towards biomarkers from liquid biopsies for early cancer detection was initiated some time ago, there are many challenges that hamper the development of such biomarkers. One of these challenges is large-scale validation that requires large prospectively collected biobanks with liquid biopsies. Establishing those biobanks involves several considerations, such as standardization of sample collection, processing and storage within and between biobanks. In this perspective, we will elaborate on several issues that need to be contemplated in biobanking, both in general and for certain specimen types specifically, to be able to facilitate biomarker validation for early detection of cancer.
Hippo-YAP1 Is a Prognosis Marker and Potentially Targetable Pathway in Advanced Gallbladder Cancer
Authors: García Patricia , Rosa Lorena , Vargas Sergio , Weber Helga , Espinoza Jaime A. , Suárez Felipe , Romero-Calvo Isabel , Elgueta Nicole , Rivera Vanessa , Nervi Bruno , Obreque Javiera , Leal Pamela , Viñuela Eduardo , Aguayo Gloria , Muñiz Sabrina , Sagredo Alfredo , Roa Juan C. , Bizama Carolina
Published: April 2020

Gallbladder cancer is an aggressive disease with late diagnosis and no efficacious treatment. The Hippo-Yes-associated protein 1 (YAP1) signaling pathway has emerged as a target for the development of new therapeutic interventions in cancers. However, the role of the Hippo-targeted therapy has not been addressed in advanced gallbladder cancer (GBC). This study aimed to evaluate the expression of the major Hippo pathway components mammalian Ste20-like protein kinase 1 (MST1), YAP1 and transcriptional coactivator with PDZ-binding motif (TAZ) and examined the effects of Verteporfin (VP), a small molecular inhibitor of YAP1-TEA domain transcription factor (TEAD) protein interaction, in metastatic GBC cell lines and patient-derived organoids (PDOs). Immunohistochemical analysis revealed that advanced GBC patients had high nuclear expression of YAP1. High nuclear expression of YAP1 was associated with poor survival in GBC patients with subserosal invasion (pT2). Additionally, advanced GBC cases showed reduced expression of MST1 compared to chronic cholecystitis. Both VP treatment and YAP1 siRNA inhibited the migration ability in GBC cell lines. Interestingly, gemcitabine resistant PDOs with high nuclear expression of YAP1 were sensitive to VP treatment. Taken together, our results suggest that key components of the Hippo-YAP1 signaling pathway are dysregulated in advanced gallbladder cancer and reveal that the inhibition YAP1 may be a candidate for targeted therapy.