bluuebunny/crossref_metadata_2025 huggingface dataset:
Variations in the Presence of Carotid Intraplaque Hemorrhage Across Age Categories: What Age Groups Are Most Likely to Benefit From Plaque Imaging?
Authors: Larson Anthony S. , Benson John C. , Brinjikji Waleed , Savastano Luis , Lanzino Giuseppe , Huston John , Nasr Deena
Published: December 2020

Although carotid artery intraplaque hemorrhage (IPH) is a known risk-factor for cerebral ischemic events in patients of advanced age, its prevalence in younger cohorts is less certain. The purpose of this study was to assess the prevalence of carotid artery IPH across the age spectrum. A retrospective review was completed of all adult patients from our institution who underwent neck MRA with high-resolution carotid plaque imaging between 2017 and 2020. The mean ages of patients with and without IPH were calculated. The prevalence of IPH was compared between patients that were categorized into age groups. Patients with and without a cerebral ischemic event (e.g., stroke, retinal ischemia) were included. Unilateral anterior circulation ischemic events in patients without atrial fibrillation were presumed to be likely related to ipsilateral carotid artery disease. Multiple regression analysis was performed to determine independent associations with IPH. 634 patients were included (1,268 carotid arteries). Increasing age (OR: 1.04; 95% CI: 1.02–1.06; P = 0.001) was independently associated with IPH. 211 patients had unilateral anterior circulation ischemic events. The mean age of patients with carotid IPH was 71.4 years (SD = 9.9), compared to 62.8 years (SD = 15.8) of those without (P ≤ 0.0001). The prevalence of IPH increased with age in all patients (P = 0.0002). Among patients with ipsilateral anterior circulation ischemic events, each age category above 50 years had a significantly higher prevalence of IPH when compared to patients 18–50 years (P ≤ 0.05 for all comparisons). The prevalence of carotid IPH increases with age and is rare in patients under 50 years. The approximate threshold age for IPH development is likely around 50 years.
Whom to Believe? Understanding and Modeling Brain Activity in Source Credibility Evaluation
Authors: Kawiak Andrzej , Wojcik Grzegorz M. , Schneider Piotr , Kwasniewicz Lukasz , Wierzbicki Adam
Published: December 2020

Understanding how humans evaluate credibility is an important scientific question in the era of fake news. Source credibility is among the most important aspects of credibility evaluations. One of the most direct ways to understand source credibility is to use measurements of brain activity of humans performing credibility evaluations. Nevertheless, source credibility has never been investigated using such a method before. This article reports the results of an experiment during which we have measured brain activity during source credibility evaluation, using EEG. The experiment allowed for identification of brain areas that were active when a participant made positive or negative source credibility evaluations. Based on experimental data, we modeled and predicted human source credibility evaluations using EEG brain activity measurements with F1 score exceeding 0.7 (using 10-fold cross-validation).
Case Report: Lymphocytosis Associated With Fatal Hepatitis in a Thymoma Patient Treated With Anti-PD1: New Insight Into the Immune-Related Storm
Authors: Argentiero Antonella , Solimando Antonio Giovanni , Ungaro Valentina , Laforgia Mariarita , Strippoli Sabino , Pinto Dario , Negri Antonio , Ferraiuolo Simona , Zito Alfredo , Guida Michele
Published: December 2020

Recent advances in tumor immunotherapy have made it possible to efficiently unleash immune effectors, reacting against neoplastic cells. Although these approaches primarily aim to eradicate malignancy, immune-related adverse events (irAEs) often influence patients’ prognosis, constituting a new spectrum of side effects. Taking into account the typical microenvironment and the intricate equilibrium between the anti-tumor response and the immune cells, the thymoma constitutes a unicum in the immune-oncology field. We report a fatal immune-mediated adverse events’ storm in a thymoma patient treated with Pembrolizumab, leading to hepatotoxicity accompanied by lymphocytosis, thrombocytopenia, and thyroid dysfunction, unveiling a novel potential pathophysiological effect of immunotherapy. The clinical proficiency of the immune checkpoint inhibitors in thymoma patients warrants timely prevention and management of off-target consequences in order to optimize this promising therapeutic option. This case report describes a unique consequence of irAEs, emerging as a red flag warranting a multidisciplinary approach.
A Novel Predictive Tool for Determining the Risk of Early Death From Stage IV Endometrial Carcinoma: A Large Cohort Study
Authors: Song Zixuan , Wang Yizi , Zhou Yangzi , Zhang Dandan
Published: December 2020

Background

Endometrial carcinoma is a common gynecological malignancy. Stage IV endometrial carcinoma is associated with a high risk of early death; however, there is currently no effective prognostic tool to predict early death in stage IV endometrial cancer.

Methods

Surveillance, Epidemiology, and End Results (SEER) data from patients with stage IV endometrial cancer registered between 2004 and 2015 were used in this study. Important independent prognostic factors were identified by univariate and multivariate logistic regression analyses. A nomogram of all-cause and cancer-specific early deaths was constructed using relevant risk factors such as tumor size, histological grade, histological classification, and treatment (surgery, radiotherapy, chemotherapy).

Results

A total of 2,040 patients with stage IV endometrial carcinoma were included in this study. Of these, 299 patients experienced early death (≤3 months) and 282 died from cancer-specific causes. The nomogram of all-cause and cancer-specific early deaths showed good predictive power and clinical practicality with respect to the area under the receiver operating characteristic curve and decision curve analysis. The internal validation of the nomogram revealed a good agreement between predicted early death and actual early death.

Conclusions

We developed a clinically useful nomogram to predict early mortality from stage IV endometrial carcinoma using data from a large cohort. This tool can help clinicians screen high-risk patients and implement individualized treatment regimens.
COVID-19 Pneumonia in Children: From Etiology to Management
Authors: Parisi Giuseppe Fabio , Indolfi Cristiana , Decimo Fabio , Leonardi Salvatore , Miraglia del Giudice Michele
Published: December 2020

COVID-19 is less serious in children than in adults. However, respiratory management dominates the clinical picture of hospitalized COVID-19 even in children. In some case series, deterioration of the clinical picture wherein dyspnea, cyanosis, and the onset of acute respiratory distress syndrome (ARDS) emerged ~8–10 days after the onset of SARS-CoV-2 infection, which could rapidly progress to multiple organ failure and death. This review aimed to evaluate the characteristics of COVID-19 pneumonia in pediatric populations, beginning from its etiology and pathological mechanisms and closing with its clinical management.
Sanguisorba officinalis L. Suppresses Triple-Negative Breast Cancer Metastasis by Inhibiting Late-Phase Autophagy via Hif-1α/Caveolin-1 Signaling
Authors: Wang Neng , Muhetaer Gulizeba , Zhang Xiaotong , Yang Bowen , Wang Caiwei , Zhang Yu , Wang Xuan , Zhang Juping , Wang Shengqi , Zheng Yifeng , Zhang Fengxue , Wang Zhiyu
Published: December 2020

Sanguisorba officinalis L. (SA) is a common herb for cancer treatment in the clinic, particularly during the consolidation phase to prevent occurrence or metastasis. Nevertheless, there are limited studies reporting the molecular mechanisms about its anti-metastatic function. It is well demonstrated that autophagy is one of the critical mechanisms accounting for metastasis and anti-cancer pharmacological actions of Chinese herbs. On the threshold, the regulatory effects and molecular mechanisms of SA in suppressing autophagy-related breast cancer metastasis were investigated in this study. In vitro findings demonstrated that SA potently suppressed the proliferation, colony formations well as metastasis process in triple-negative breast cancer. Network and biological analyses predicted that SA mainly targeted caveolin-1 (Cav-1) to induce anti-metastatic effects, and one of the core mechanisms was via regulation of autophagy. Further experiments—including western blotting, transmission electron microscopy, GFP-mRFP-LC3 immunofluorescence, and lysosomal-activity detection—validated SA as a potent late-stage autophagic inhibitor by increasing microtubule-associated light chain 3-II (LC3-II) conversion, decreasing acidic vesicular-organelle formation, and inducing lysosomal dysfunction even under conditions of either starvation or hypoxia. Furthermore, the anti-autophagic and anti-metastatic activity of SA was Cav-1-dependent. Specifically, Cav-1 knockdown significantly facilitated SA-mediated inhibition of autophagy and metastasis. Furthermore, hypoxia inducible factor-1α (Hif-1α) overexpression attenuated the SA-induced inhibitory activities on Cav-1, autophagy, and metastasis, indicating that SA may have inhibited autophagy-related metastasis via Hif-1α/Cav-1 signaling. In both mouse breast cancer xenograft and zebrafish xenotransplantation models, SA inhibited breast cancer growth and inhibited late-phase autophagy in vivo, which was accompanied by suppression of Hif-1α/Cav-1 signaling and the epithelial-mesenchymal transition. Overall, our findings not only indicate that SA acts as a novel late-phase autophagic inhibitor with anti-metastatic activities in triple-negative breast cancer, but also highlight Cav-1 as a regulator in controlling late-phase autophagic activity.
Fast-Velocity Eccentric Cycling Exercise Causes Greater Muscle Damage Than Slow Eccentric Cycling
Authors: Ueda Hisashi , Tsuchiya Yosuke , Ochi Eisuke
Published: December 2020

This study aims to investigate muscle damage occurring in the early and recovery phases after fast-velocity and slow-velocity eccentric cycling. Eleven untrained men (age, 20.0 ± 1.7 years; height, 171.3 ± 6.8 cm; weight, 61.8 ± 7.7 kg; and %body fat, 13.2 ± 2.9%) performed slow-velocity maximal isokinetic eccentric cycling (slow-velocity; 30°/s) with one leg and fast-velocity (fast-velocity; 210°/s) isokinetic eccentric cycling with the other leg. Changes in maximal voluntary isokinetic concentric contraction (MVCC) torque at velocities of 30 and 210°/s, range of motion (ROM), and muscle soreness were assessed by pressure using a digital muscle stiffness instrument; thigh circumference, muscle echo intensity, and muscle stiffness were assessed before exercise, and immediately after exercise, 1 day, and 4 days after exercise. Comparing with the results obtained for slow-velocity cycling (post: 215.9 ± 32.3 Nm, day 1: 192.9 ± 47.4 Nm, day 4: 184.3 ± 47.2 Nm) and before exercise, MVCC after fast-velocity cycling significantly decreased at immediately (160.4 ± 43.5 Nm), 1 day (143.6 ± 54.1 Nm), and 4 days (150.1 ± 44.5 Nm) after exercise (p < 0.05). Significant increase in muscle soreness for vastus lateralis was observed after fast-velocity cycling (41.2 ± 16.9 mm) compared with slow-velocity cycling (23.7 ± 12.2 mm) 4 days after exercise (p < 0.05). However, no significant difference in muscle soreness was observed for rectus femoris and vastus medialis at any time points after exercise. In addition, no significant differences were observed in the ROM, thigh circumference, muscle echo intensity, and muscle stiffness. In conclusion, fast-velocity eccentric cycling causes a decrease in muscle strength and an increase in soreness as compared to slow-velocity eccentric cycling.
Chloroplast Electron Chain, ROS Production, and Redox Homeostasis Are Modulated by COS-OGA Elicitation in Tomato (Solanum lycopersicum) Leaves
Authors: Moreau Sophie , van Aubel Géraldine , Janky Rekin’s , Van Cutsem Pierre
Published: December 2020

The stimulation of plant innate immunity by elicitors is an emerging technique in agriculture that contributes more and more to residue-free crop protection. Here, we used RNA-sequencing to study gene transcription in tomato leaves treated three times with the chitooligosaccharides–oligogalacturonides (COS-OGA) elicitor FytoSave® that induces plants to fend off against biotrophic pathogens. Results showed a clear upregulation of sequences that code for chloroplast proteins of the electron transport chain, especially Photosystem I (PSI) and ferredoxin. Concomitantly, stomatal conductance decreased by half, reduced nicotinamide adenine dinucleotide phosphate [NAD(P)H] content and reactive oxygen species production doubled, but fresh and dry weights were unaffected. Chlorophyll, β-carotene, violaxanthin, and neoxanthin contents decreased consistently upon repeated elicitations. Fluorescence measurements indicated a transient decrease of the effective PSII quantum yield and a non-photochemical quenching increase but only after the first spraying. Taken together, this suggests that plant defense induction by COS-OGA induces a long-term acclimation mechanism and increases the role of the electron transport chain of the chloroplast to supply electrons needed to mount defenses targeted to the apoplast without compromising biomass accumulation.
Accurate Detection and Evaluation of the Gene-Editing Frequency in Plants Using Droplet Digital PCR
Authors: Peng Cheng , Zheng Ming , Ding Lin , Chen Xiaoyun , Wang Xiaofu , Feng Xuping , Wang Junmin , Xu Junfeng
Published: December 2020

Gene-editing techniques are becoming powerful tools for modifying target genes in organisms. Although several methods have been reported that detect mutations at targeted loci induced by the CRISPR/Cas system in different organisms, they are semiquantitative and have difficulty in the detection of mutants in processed food samples containing low initial concentrations of DNA and may not accurately quantify editing frequency, especially at very low frequencies in a complex polyploid plant genome. In this study, we developed a duplexed dPCR-based method for the detection and evaluation of gene-editing frequencies in plants. We described the design, performance, accurate quantification, and comparison with other detection systems. The results show that the dPCR-based method is sensitive to different kinds of gene-editing mutations induced by gene-editing. Moreover, the method is applicable to polyploid plants and processed food samples containing low initial concentrations of DNA. Compared with qPCR and NGS-based methods, the dPCR method has a lower limit of detection (LOD) of the editing frequency and a better relationship with the expected editing frequency in detecting the edited region of gene-edited rice samples. Taken together, the duplexed dPCR assay is accurate and precise, and it will be a powerful tool for the detection and evaluation of gene-editing frequencies in plants in gene-editing technology.
Blockchain Technology for Sustainable Waste Management
Authors: Taylor Phillip , Steenmans Katrien , Steenmans Ine
Published: December 2020

The global waste and resource crises necessitate and give great impetus for better and more sustainable management of waste. Increasingly, resource and waste streams that once were sent to landfill or incinerated are now reused, recycled, or recovered. Yet, while many laws and policies have been adopted for this very purpose, a number of recurrent challenges persist across interventions seeking to further facilitate the necessary, widespread transitions to sustainable waste management. This perspective article explores the suitability of blockchain technology in overcoming these challenges. In particular, we discuss the opportunities and challenges for blockchain in (1) offering clarity in property rights of products and wastes, (2) supporting law and policy goals by incentivizing sustainable waste management, and (3) maintaining anonymity and privacy for institutions and individuals.
Measuring Perseverance and Passion in Distance Education Students: Psychometric Properties of the Grit Questionnaire and Associations With Academic Performance
Authors: Xu Kate M. , Meijs Celeste , Gijselaers Hieronymus J. M. , Neroni Joyce , de Groot Renate H. M.
Published: December 2020

With modern technological advances, distance education has become an increasingly important education delivery medium for, for example, the higher education provided by open universities. Among predictive factors of successful learning in distance education, the effects of non-cognitive skills are less explored. Grit, the dispositional tendency to sustain trait-level passion and long-term goals, has raised much research interest and gained importance for predicting academic achievement. The Grit Questionnaire, measuring Perseverance of Effort and Consistency of Interests, has been shown to be a reliable instrument in traditional university student populations. However, the measurement and predictive validity of this questionnaire is still unknown for adult distance education university students who differ from traditional students in various ways (e.g., having a wider range of student ages). Based on a sample of 2,027 students from a distance education university, this study assessed the psychometric properties of the two-factor structure grit measured by the Grit Questionnaire. The findings suggest that the short form of the Grit Questionnaire is a potentially useful assessment tool for measuring the grit construct for distance learning higher education and that the Consistency of Interests factor is especially relevant to consider the improvement of learning performance for distance education in terms of courses credit and exam attempts. The measurement precision of the Perseverance of Effort factor, however, should be improved in future research to provide higher measurement accuracy and broader item coverage.
How Political and Social Trust Can Impact Social Distancing Practices During COVID-19 in Unexpected Ways
Authors: Woelfert Frederike S. , Kunst Jonas R.
Published: December 2020

In times of the coronavirus, complying with public health policies is essential to save lives. Understanding the factors that influence compliance with social distancing measures is therefore an urgent issue. The present research investigated the role of political and social trust for social distancing using a variety of methods. In Study 1 (N = 301), conducted with a sample from the United Kingdom in the midst of the virus outbreak (i.e., the first wave), neither political nor social trust had main associations with self-reported social distancing tendencies. However, both factors interacted such that social trust was associated with lower social distancing tendencies among participants with low levels of political trust. In Study 2, using an experimental longitudinal design and again conducted with a sample collected from the UK (N = 268) during the first wave of the pandemic, social distancing practices increased over time, independent of an experimental manipulation of political trust. Moreover, while the interaction between political and social trust from the first study could not be conceptually replicated, social trust was positively related to social distancing intentions. Moving from the individual to the country level and assessing actual behavior at both the first and second wave of the pandemic, in Study 3 (N = 65 countries), country-level political trust was related to less social distancing during the first wave. Social trust was related to a higher growth rate of infections. Against the background of these inconsistent findings, we discuss the potential positive and unexpected negative effects of social trust for social distancing.
Scrutinizing Social Identity Theory in Corporate Social Responsibility: An Experimental Investigation
Authors: Paruzel Agnieszka , Danel Martin , Maier Günter W.
Published: December 2020

Corporate social responsibility (CSR) is widely established by companies that aim to contribute to society and minimize their negative impact on the environment. In CSR research, employees’ reactions to CSR have extensively been researched. Social identity theory is often used as a theoretical background to explain the relationship between CSR and employee-related outcomes, but until now, a sound empirical examination is lacking, and causality remains unclear. CSR can unfold its effect mainly because of three theoretically important aspects of CSR initiatives, which increase identification, i.e., distinctiveness, prestige, and salience of the out-group. This study examines how far identification can explain the effect of CSR on employees. In an experimental vignette study (N = 136 employees), CSR was manipulated in three degrees (positive, neutral, and negative) to examine its effects on job satisfaction, organizational commitment, and organizational citizenship behavior (OCB). In the vignettes, information on distinctiveness, prestige, and salience of the out-group were presented. Regression analyses showed that CSR significantly predicted commitment and job satisfaction, but not OCB. We found mediation effects of CSR on commitment, job satisfaction, and OCB through identification, but the effect of CSR on identification explained only little variance which indicates additional underlying mechanisms. The applicability of social identity theory for explaining CSR is discussed. Moreover, we discuss further explaining mechanisms.
Understanding the Wellbeing Effects of a Community Music Program for People With Disabilities: A Mixed Methods, Person-Centered Study
Authors: MacGlone Una M. , Vamvakaris Joy , Wilson Graeme B. , MacDonald Raymond A. R.
Published: December 2020

People with disabilities face inequalities in mental wellbeing, for which social exclusion is a contributing factor. Musical activities offer a promising but complex intervention, making impacts on a population with highly varied characteristics and needs challenging to capture. This paper reports on a mixed methods, person-centered study investigating a community music intervention for such a population. Three groups of adult service users with varied disabilities (either physical, learning, or both), took part in weekly music workshops in different locations. Music staff, housing and resource center staff, as well as participants and members of their families, took part in semi-structured interviews. A quantitative measure administered by service staff was used to rate service users’ social development. Two lay researchers, both individuals with a disability contributed to all aspects of the study. Interviews were analyzed through thematic analysis. Improvements in individuals’ self-expression, confidence, mood, and social skills were consistent with previous findings. Differences in effect between centers included: Group 1, some of whom had previous experience of workshops, showed an improvement in musical skills; Group 2 showed a mixed response, some participated with enthusiasm but others chose art activities over music workshops; Group 3 had lasting positive impact, this group had very limited opportunities for music due to their rural location. Quantitative analysis showed significant increase over all groups in communication, interaction with others, and joint attention. The intervention was beneficial for participants in separate locations in similar ways, but also highlighted that context and prior experience mediated effects in distinct ways. The lay researchers enhanced the qualitative analysis by emphasizing (1) the importance of recognizing participants’ self-expression in non-verbal modes of communication and (2) the importance of having music staff with a disability to provide a positive role model. This paper proposes that mixed methods person-centered research is the most suitable approach to capture and understand the multiple and varied effects of this complex intervention for a diverse group of participants.
Predictive Effect of Positive Youth Development Attributes on Delinquency Among Adolescents in Mainland China
Authors: Zhu Xiaoqin , Shek Daniel T. L.
Published: December 2020

The general proposition of the positive youth development (PYD) approach is that developmental assets such as psychosocial competence can promote healthy adolescent development and reduce problem behavior. Despite that many Western studies have shown that PYD attributes are negatively related to adolescent delinquency, not all empirical findings support the negative associations. Although different dimensions of PYD attributes may bear differential relationships with delinquency, this possibility has not been properly examined so far. In addition, related studies in mainland China do not exist. Finally, the possible mediating role of life satisfaction in linking PYD attributes to delinquency has rarely been studied. To address the research gaps and understand how PYD attributes are associated with adolescent delinquency and the underlying mediating effect of life satisfaction, matched longitudinal data were collected from 2,648 mainland Chinese secondary school students (1,109 girls, Mean age = 13.12 ± 0.81 years at Wave 1) at two waves which were separated by one year. On each occasion, participants completed a questionnaire containing validated measures of PYD attributes, life satisfaction, and delinquency. Congruent with the general theoretical prediction of the PYD approach, different PYD attributes were inversely related to concurrent and future adolescent delinquency in separate regression analyses. In addition, the negative predictions were mediated by life satisfaction. When all PYD attributes were included in a single path analysis model, three findings were observed. First, two PYD dimensions, including self-identity and general PYD attributes, showed robust negative predictions on delinquency via life satisfaction. Second, prosocial attributes displayed a weak and unstable negative predictive effect. Third, cognitive-behavioral competence showed an unexpected positive predictive effect on delinquency directly or via its negative effects on life satisfaction. The present findings add value to the existing literature by revealing the predictive role of PYD attributes on life satisfaction and delinquency among mainland Chinese adolescents. The findings also reinforce the importance of investigating individual dimensions of PYD attributes simultaneously in the research field. The present study suggests that it is promising to cultivate PYD attributes as a strategy to reduce delinquency among adolescents in mainland China.
Impulsivity and Venturesomeness in an Adult ADHD Sample: Relation to Personality, Comorbidity, and Polygenic Risk
Authors: Grimm Oliver , Weber Heike , Kittel-Schneider Sarah , Kranz Thorsten M. , Jacob Christian P. , Lesch Klaus-Peter , Reif Andreas
Published: December 2020

While impulsivity is a basic feature of attention-deficit/hyperactivity disorder (ADHD), no study explored the effect of different components of the Impulsiveness (Imp) and Venturesomeness (Vent) scale (IV7) on psychiatric comorbidities and an ADHD polygenic risk score (PRS). We used the IV7 self-report scale in an adult ADHD sample of 903 patients, 70% suffering from additional comorbid disorders, and in a subsample of 435 genotyped patients. Venturesomeness, unlike immediate Impulsivity, is not specific to ADHD. We consequently analyzed the influence of Imp and Vent also in the context of a PRS on psychiatric comorbidities of ADHD. Vent shows a distinctly different distribution of comorbidities, e.g., less anxiety and depression. PRS showed no effect on different ADHD comorbidities, but correlated with childhood hyperactivity. In a complementary analysis using principal component analysis with Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition ADHD criteria, revised NEO Personality Inventory, Imp, Vent, and PRS, we identified three ADHD subtypes. These are an impulsive–neurotic type, an adventurous–hyperactive type with a stronger genetic component, and an anxious–inattentive type. Our study thus suggests the importance of adventurousness and the differential consideration of impulsivity in ADHD. The genetic risk is distributed differently between these subtypes, which underlines the importance of clinically motivated subtyping. Impulsivity subtyping might give insights into the organization of comorbid disorders in ADHD and different genetic background.
Among Persons With Multiple Sclerosis (MS), Objective Sleep, Psychological Functioning, and Higher Physical Activity Scores Remained Stable Over 2 Years—Results From a Small Study Under Naturalistic Conditions
Authors: Sadeghi Bahmani Dena , Gonzenbach Roman , Kesselring Jürg , Bansi Jens , Motl Robert W. , Cordier Dominik , Rothen Oliver , Niedermoser Daryl , Gerber Markus , Brand Serge
Published: December 2020

Background: Persons with multiple sclerosis (PwMS) are at increased risk to report poor sleep patterns and lower physical activity indices. To date, data on longitudinal objectively sleep assessment is missing. In the present study, we investigated the pattern of objective sleep and subjective physical activity indices over a period of 13.5 months, under naturalistic conditions.

Method: 13.5 months after their first assessment, a total of 16 PwMS (mean age = 49.13 median EDSS score: 5; 11 females) were reassessed on their objective sleep via portable sleep-electroencephalogram (EEG-) devices, along with their subjective sleep patterns (symptoms of insomnia, restless legs syndrome (RLS), and sleep-disordered breathing), physical activity indices, psychological functioning (symptoms of depression, fatigue, daytime sleepiness), and MS-related information (fatigue, EDSS; disease-modifying treatments). While the baseline assessment was performed in a rehabilitation center, the follow-up assessment took place at participants' naturalistic and familiar setting.

Results: Statistically, symptoms of depression and fatigue, subjective sleep, and physical activity levels did neither increase, nor decrease over time, although descriptively, both moderate and vigorous physical activity levels decreased, and fatigue and subjective insomnia increased. Time awake after sleep onset statistically significantly decreased, while light sleep duration increased by trend.

Conclusions: Among a smaller sample of PwMS, objective sleep in their naturalistic setting remained fairly stable over a mean time lapse of 13.5 months after clinic discharge. Physical activity levels descriptively decreased. The present results are of clinical and practical importance for treatment counseling: PwMS can be reassured that their sleep quality does not deteriorate, once they have left a rehabilitation center. Further, they should be encouraged to keeping their physical activity levels as stable as possible.
Arsenic Exposure and Cancer-Related Proteins in Urine of Indigenous Bolivian Women
Authors: De Loma Jessica , Gliga Anda R. , Levi Michael , Ascui Franz , Gardon Jacques , Tirado Noemi , Broberg Karin
Published: December 2020

Indigenous people living in the Bolivian Andes are exposed through their drinking water to inorganic arsenic, a potent carcinogen. However, the health consequences of arsenic exposure in this region are unknown. The aim of this study was to evaluate associations between arsenic exposure and changes in cancer-related proteins in indigenous women (n = 176) from communities around the Andean Lake Poopó, Bolivia. Arsenic exposure was assessed in whole blood (B-As) and urine (as the sum of arsenic metabolites, U-As) by inductively coupled plasma-mass spectrometry (ICP-MS). Cancer-related proteins (N = 92) were measured in urine using the proximity extension assay. The median B-As concentration was 2.1 (range 0.60–9.1) ng/g, and U-As concentration was 67 (12–399) μg/L. Using linear regression models adjusted for age, urinary osmolality, and urinary leukocytes, we identified associations between B-As and four putative cancer-related proteins: FASLG, SEZ6L, LYPD3, and TFPI2. Increasing B-As concentrations were associated with lower protein expression of SEZ6L, LYPD3, and TFPI2, and with higher expression of FASLG in urine (no association was statistically significant after correcting for multiple comparisons). The associations were similar across groups with different arsenic metabolism efficiency, a susceptibility factor for arsenic toxicity. In conclusion, arsenic exposure in this region was associated with changes in the expression of some cancer-related proteins in urine. Future research is warranted to understand if these proteins could serve as valid biomarkers for arsenic-related toxicity.
A Framework for Automatic Behavior Generation in Multi-Function Swarms
Authors: Engebraaten Sondre A. , Moen Jonas , Yakimenko Oleg A. , Glette Kyrre
Published: December 2020

Multi-function swarms are swarms that solve multiple tasks at once. For example, a quadcopter swarm could be tasked with exploring an area of interest while simultaneously functioning as ad-hoc relays. With this type of multi-function comes the challenge of handling potentially conflicting requirements simultaneously. Using the Quality-Diversity algorithm MAP-elites in combination with a suitable controller structure, a framework for automatic behavior generation in multi-function swarms is proposed. The framework is tested on a scenario with three simultaneous tasks: exploration, communication network creation and geolocation of Radio Frequency (RF) emitters. A repertoire is evolved, consisting of a wide range of controllers, or behavior primitives, with different characteristics and trade-offs in the different tasks. This repertoire enables the swarm to online transition between behaviors featuring different trade-offs of applications depending on the situational requirements. Furthermore, the effect of noise on the behavior characteristics in MAP-elites is investigated. A moderate number of re-evaluations is found to increase the robustness while keeping the computational requirements relatively low. A few selected controllers are examined, and the dynamics of transitioning between these controllers are explored. Finally, the study investigates the importance of individual sensor or controller inputs. This is done through ablation, where individual inputs are disabled and their impact on the performance of the swarm controllers is assessed and analyzed.
Vadose Zone Monitoring as a Key to Groundwater Protection
Authors: Dahan Ofer
Published: December 2020

Currently, monitoring programs designed for groundwater protection are mainly based on information from observation wells. This, however, creates a paradox, since identification of pollution in well water is clear evidence that the groundwater is already polluted. The poor state of contaminated aquifers all over the world, and the inability, in practice, to fully remediate contaminated aquifers suggest that groundwater monitoring alone has failed to provide the vital information required to prevent groundwater pollution. That said, groundwater pollution initiates on the land surface, and the contaminants have to traverse the unsaturated zone, long before reaching the water table. Therefore, monitoring programs that can provide real-time information on the hydraulic and chemical state of the unsaturated zone are essential for achieving early warnings of pollution potential and providing imperative protection from pollution hazards. Currently, most of the commercially available monitoring technologies are rather limited in their capability to provide early alerts of pollution processes taking place deep in the unsaturated zone, above the water table. Accordingly, monitoring technologies for the unsaturated zone have to be engineered as “off-the-shelf” commercial products, made available for application by practitioners in all fields of hydrology. From scientific and technological points of view, such ambitions are not out of reach. Yet they require an urgent call for a revolutionary shift in monitoring focus, from the groundwater itself to the unsaturated zone above it.
Dietary Probiotics or Synbiotics Supplementation During Gestation, Lactation, and Nursery Periods Modifies Colonic Microbiota, Antioxidant Capacity, and Immune Function in Weaned Piglets
Authors: Wang Kai , Zhu Qian , Kong Xiangfeng , Song Mingtong , Azad Md. Abul Kalam , Xiong Liang , Zheng Yuzhong , He Qinghua
Published: December 2020

This study was conducted to investigate the effect of dietary probiotics or synbiotics supplementation on colonic microbiota, antioxidant capacity, and immune function in weaned piglets. A total of 64 pregnant Bama mini-sows and then 128 of their weaned piglets were randomly assigned into control group, antibiotics group, probiotics group, or synbiotics group. The results showed that colonic Firmicutes and Bifidobacterium abundances in the probiotics group and total bacteria, Bacteroidetes, and Lactobacillus abundances in the synbiotics group were increased (P < 0.05), while Escherichia coli abundance in the synbiotics group was decreased (P = 0.061) compared with the control group. Firmicutes, Bifidobacterium, and total bacteria abundances were increased (P < 0.05) in the probiotics and synbiotics groups compared with the antibiotics group. Probiotics supplementation up-regulated (P < 0.05) the mRNA expression of GPR109A compared with the control and antibiotics groups. Dietary probiotics or synbiotics supplementation improved the antioxidant capacity by increasing (P < 0.05) the colonic CAT, GSH-Px, SOD, and T-AOC levels and plasma CAT, GSH, GSH-Px, and SOD levels and by decreasing (P < 0.05) the colonic and plasma MDA and H2O2 levels. Compared to the control group, the colonic IL-10, IFN-α, and sIgA concentrations and plasma IgA and IgM concentrations were significantly increased (P < 0.05) in the probiotics and synbiotics groups. Spearman's correlation analysis showed that the changed colonic microbiota, such as Lactobacillus and Bifidobacterium were correlated with the alteration of antioxidant indexes, cytokines, and immunoglobulins. In conclusion, dietary probiotics or synbiotics supplementation during gestation, lactation, and nursery periods could be used as an alternative for antibiotics in terms of gut health of weaned piglets.
Effect of Quercetin Supplementation in Extender on Sperm Kinematics, Extracellular Enzymes Release, and Oxidative Stress of Egyptian Buffalo Bulls Frozen–Thawed Semen
Authors: El-Khawagah Ahmed R. M. , Kandiel Mohamed M. M. , Samir Haney
Published: December 2020

Buffalo spermatozoa are more sensitive for cryopreservation compared to other species. This study aimed to evaluate the consequences of quercetin against cryodamage of buffalo frozen–thawed spermatozoa characteristics. Semen of Egyptian bulls (n= 4) was extended in OptiXcell extender incorporated with quercetin at 0 (control), 2.5, 5.0, 10.0, 20.0, 40.0, and 80.0 μM before cryopreservation. Frozen–thawed semen was evaluated for sperm motility by computer-assisted sperm analyzer (CASA), viability, morphology, membrane, and acrosome integrities. The kinematics parameters including average path velocity (VAP; μm/s), straight linear velocity (VSL; μm/s), curvilinear velocity (VCL; μm/s), amplitude of lateral head displacement (ALH; μm), beat cross frequency (BCF; Hz), linearity [LIN, (VSL/VCL) × 100], and straightness [STR, (VSL/VAP) × 100] were assessed. The sperm-free extender was evaluated for aspartate aminotransferase (AST), alanine aminotransferase (ALT), and H2O2. Homogenized sperm cells were evaluated for oxidative stress biomarkers [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX)], and lipid peroxidation [malondialdehyde (MDA)]. The highest values of total motility, progressive motility, viability, intact acrosome, and membrane integrity substantially improved with 10 μM of quercetin. STR (%) was substantially low (P< 0.01), and VCL (μm/s) and ALH (μm) were markedly high (P< 0.05) in 10 μM of quercetin. The outflow of ALT enzyme to extracellular fluid was lower with 10 μM of quercetin (P< 0.001) and higher at 2.5 μM of quercetin. The spermatozoa leaked AST was markedly lower at 5.0, 10 (P< 0.001) and 20 μM (P< 0.05) of quercetin. The activity of antioxidant enzymes was eminently low at all quercetin concentrations, and this was accompanied by the decrease in H2O2in the media. SOD activity at 10–80 μM, CAT at 5.0–40 μM, and GPX at 2.5–80.0 μM of quercetin in spermatozoa were substantially low. MDA level significantly (P< 0.001) decreased at all quercetin concentrations. In conclusion, the incorporation of quercetin at the level of 10 μM is promising in improving buffalo semen characteristics and lower the freezing–thawing oxidative stress.
Feature Selection from Lyme Disease Patient Survey Using Machine Learning
Authors: Vendrow Joshua , Haddock Jamie , Needell Deanna , Johnson Lorraine
Published: December 2020

Lyme disease is a rapidly growing illness that remains poorly understood within the medical community. Critical questions about when and why patients respond to treatment or stay ill, what kinds of treatments are effective, and even how to properly diagnose the disease remain largely unanswered. We investigate these questions by applying machine learning techniques to a large scale Lyme disease patient registry, MyLymeData, developed by the nonprofit LymeDisease.org. We apply various machine learning methods in order to measure the effect of individual features in predicting participants’ answers to the Global Rating of Change (GROC) survey questions that assess the self-reported degree to which their condition improved, worsened, or remained unchanged following antibiotic treatment. We use basic linear regression, support vector machines, neural networks, entropy-based decision tree models, and k-nearest neighbors approaches. We first analyze the general performance of the model and then identify the most important features for predicting participant answers to GROC. After we identify the “key” features, we separate them from the dataset and demonstrate the effectiveness of these features at identifying GROC. In doing so, we highlight possible directions for future study both mathematically and clinically.
Design and Evaluation of a Pediatric Lower-Limb Exoskeleton Joint Actuator
Authors: Goo Anthony , Laubscher Curt A. , Farris Ryan J. , Sawicki Jerzy T.
Published: December 2020

Lower-limb exoskeletons have undergone significant developments for aiding in the ambulation of adults with gait impairment. However, advancements in exoskeletons for the pediatric population have comparatively been lacking. This paper presents a newly developed joint actuator designed to drive the hip and knee joints of a pediatric lower-limb exoskeleton. The performance requirements associated with the actuators were determined based on a target audience of children ages 6–11 years old. The developed actuators incorporate a hybrid belt-chain transmission driven by a frameless brushless DC motor. One actuator underwent benchtop testing to evaluate its performance with respect to their torque production, bandwidth properties, backdrivability in terms of inertia and friction characteristics, speed capabilities, and operational noise levels. As a preliminary validation, a set of actuators were placed in a prototype orthosis to move a pediatric test dummy in gait tracking via state-feedback control. The results showed that the newly developed actuators meet the design specifications and are suitable for use in the pediatric exoskeleton being developed.
Analysis of Aircraft Maintenance Related Accidents and Serious Incidents in Nigeria
Authors: Habib Khadijah , Turkoglu Cengiz
Published: December 2020

The maintenance of aircraft presents considerable challenges to the personnel that maintain them. Challenges such as time pressure, system complexity, sparse feedback, cramped workspaces, etc., are being faced by these personnel on a daily basis. Some of these challenges cause aircraft-maintenance-related accidents and serious incidents. However, there is little formal empirical work that describes the influence of aircraft maintenance to aircraft accidents and incidents in Nigeria. This study, therefore, sets out to explore the contributory factors to aircraft-maintenance-related incidents from 2006 to 2019 and accidents from 2009 to 2019 in Nigeria, to achieve a deeper understanding of this safety critical aspect of the aviation industry, create awareness amongst the relevant stakeholders and seek possible mitigating factors. To attain this, a content analysis of accident reports and mandatory occurrence reports, which occurred in Nigeria, was carried out using the Maintenance Factors and Analysis Classification System (MxFACS) and Hieminga’s maintenance incidents taxonomy. An inter-rater concordance value was used to ascertain research accuracy after evaluation of the data output by subject matter experts. The highest occurring maintenance-related incidents and accidents were attributed to “removal/installation”, working practices such as “accumulation of dirt and contamination”, “inspection/testing”, “inadequate oversight from operator and regulator”, “failure to follow procedures” and “incorrect maintenance”. To identify the root cause of these results, maintenance engineers were consulted via a survey to understand the root causes of these contributory factors. The results of the study revealed that the most common maintenance-related accidents and serious incidents in the last decade are “collision with terrain” and “landing gear events’’. The most frequent failures at systems level resulting in accidents are the “engines” and “airframe structure”. The maintenance factors with the highest contribution to these accidents are “operator and regulatory oversight”, “inadequate inspection” and “failure to follow procedures”. The research also highlights that the highest causal and contributory factors to aviation incidents in Nigeria from 2006 to 2019 are “installation/removal issues”, “inspection/testing issues”, “working practices”, “job close up”, “lubrication and servicing”, all of which corresponds to studies by other researchers in other countries.
Mechanism of Plant Growth Promotion and Disease Suppression by Chitosan Biopolymer
Authors: Chakraborty Moutoshi , Hasanuzzaman Mirza , Rahman Mahfuzur , Khan Md. Arifur Rahman , Bhowmik Pankaj , Mahmud Nur Uddin , Tanveer Mohsin , Islam Tofazzal
Published: December 2020

The chitosan (CHT) biopolymer is a de-acetylated chitin derivative that exists in the outer shell of shrimp, shellfish, lobster or crabs, as well as fungal cell walls. Because of its biodegradability, environmental non-toxicity, and biocompatibility, it is an ideal resource for sustainable agriculture. The CHT emerged as a promising agent used as a plant growth promoter and also as an antimicrobial agent. It induces plant growth by influencing plant physiological processes like nutrient uptake, cell division, cell elongation, enzymatic activation and synthesis of protein that can eventually lead to increased yield. It also acts as a catalyst to inhibit the growth of plant pathogens, and alter plant defense responses by triggering multiple useful metabolic pathways. This review emphasizes the role and mechanisms of CHT as a plant growth promoter and disease suppressor, and its future implications in agriculture.