bluuebunny/crossref_metadata_2025 huggingface dataset:
Induction of Cell Death in the Human Acute Lymphoblastic Leukemia Cell Line Reh by Infection with Rotavirus Isolate Wt1-5
Authors: Guerrero Rafael , Guerrero Carlos , Acosta Orlando
Published: July 2020

Cancer is a major health problem that poses a great challenge to health care systems worldwide. Tools for cancer treatment have rapidly advanced in recent years, resulting in therapeutic strategies which are alternative and complementary to conventional treatment. To identify the cell surface receptors used by a tumor cell-adapted rotavirus and the cell death markers induced by its infection, we use Wt1-5, a rotavirus isolate recently adapted to tumor cells, to infect the human acute lymphoblastic leukemia cell line, Reh. The expression of cell surface receptors used by Wt1-5 was determined using flow cytometry and an antibody blocking assay to test for their implication in virus infection. Viral antigens and cell death markers induced by rotavirus infection were followed by flow cytometric analysis. The present study showed that rotavirus Wt1-5 was able to use cell surface proteins such as heat shock proteins (HSPs) 90, 70, 60 and 40, Hsc70, PDI and integrin β3. Rotavirus Wt1-5 induced cytotoxic effects including changes in cell membrane permeability, alteration of mitochondrial membrane potential, DNA fragmentation and activation of cell death signaling. Wt1-5 deserves to be further studied as a candidate oncolytic agent due to its ability to induce apoptosis in lymphoblastic leukemia-derived cells.
Effects of Neuromuscular Electrical Stimulation (NMES) Plus Upper Cervical Spine Mobilization on Forward Head Posture and Swallowing Function in Stroke Patients with Dysphagia
Authors: Jeon Yung Hyun , Cho Kyun Hee , Park Shin Jun
Published: July 2020

After a stroke, forward head posture occurs, resulting in swallowing dysfunction. Neuromuscular electrical stimulation (NMES) combined with upper cervical spine mobilization has demonstrated enhanced recovery of the swallowing function in stroke patients. This study investigated the therapeutic effects of NMES in conjunction with upper cervical mobilization in stroke patients with dysphagia. Thirty-four stroke patients were recruited (in a randomized controlled clinical trial) and divided into an experimental group (n = 17; NMES plus upper cervical spine mobilization) and a control group (n = 17; NMES plus sham mobilization). Forward head posture was measured by craniocervical flexion test (CCFT) and craniovertebral angle (CVA). Swallowing function was measured by variations in video fluoroscopic dysphagia scale (VDS) and penetration–aspiration scale (PAS) scores using the video fluoroscopic swallowing study (VFSS). All measurements were done at baseline and after four weeks of NMES plus mobilization. A significant increase was observed in CCFT, CVA, VDS (total VDS score, oral stage score, pharyngeal stage score), and PAS score in all variations in the experimental group. The CCFT, CVA, pharyngeal stage score, total VDS, and PAS score were significantly higher in the experimental group when compared to the control group. NMES plus upper cervical spine mobilization can be regarded as a promising method to improve swallowing function and forward head posture changes in stroke patients with dysphagia.
Definition of Optimal Ventilation Rates for Balancing Comfort and Energy Use in Indoor Spaces Using CO2 Concentration Data
Authors: Franco Alessandro , Schito Eva
Published: July 2020

Air ventilation rate plays a relevant role in maintaining adequate indoor air quality (IAQ) conditions in public buildings. In general, high ventilation rates ensure good indoor air quality but entail relevant energy consumption. Considering the necessity of balancing IAQ and energy consumption, a correlation between the number of occupants obtained from analysis of CO2 concentration variation is presented as a general element for controlling the operation of heating ventilation and air cooling (HVAC) systems. The specific CO2 exhalation rate is estimated using experimental data in some real conditions in university classrooms. A method for the definition of optimal values of air exchange rate is defined, highlighting that the obtained values are much lower than those defined in current technical standards with possibilities of relevant reduction of the total energy consumption.
Non-Invasive Assessment of Hypoxia and Neovascularization with MRI for Identification of Aggressive Breast Cancer
Authors: Bennani-Baiti Barbara , Pinker Katja , Zimmermann Max , Helbich Thomas H. , Baltzer Pascal A. , Clauser Paola , Kapetas Panagiotis , Bago-Horvath Zsuzsanna , Stadlbauer Andreas
Published: July 2020

The aim of this study was to investigate the potential of magnetic resonance imaging (MRI) for a non-invasive synergistic assessment of tumor microenvironment (TME) hypoxia and induced neovascularization for the identification of aggressive breast cancer. Fifty-three female patients with breast cancer underwent multiparametric breast MRI including quantitative blood-oxygen-level-dependent (qBOLD) imaging for hypoxia and vascular architecture mapping for neovascularization. Quantitative MRI biomarker maps of oxygen extraction fraction (OEF), metabolic rate of oxygen (MRO2), mitochondrial oxygen tension (mitoPO2), microvessel radius (VSI), microvessel density (MVD), and microvessel type indicator (MTI) were calculated. Histopathology was the standard of reference. Histopathological markers (vascular endothelial growth factor receptor 1 (FLT1), podoplanin, hypoxia-inducible factor 1-alpha (HIF-1alpha), carbonic anhydrase 9 (CA IX), vascular endothelial growth factor C (VEGF-C)) were used to confirm imaging biomarker findings. Univariate and multivariate regression analyses were performed to differentiate less aggressive luminal from aggressive non-luminal (HER2-positive, triple negative) malignancies and assess the interplay between hypoxia and neoangiogenesis markers. Aggressive non-luminal cancers (n = 40) presented with significantly higher MRO2 (i.e., oxygen consumption), lower mitoPO2 values (i.e., hypoxia), lower MTI, and higher MVD than less aggressive cancers (n = 13). Data suggest that a model derived from OEF, mitoPO2, and MVD can predict tumor proliferation rate. This novel MRI approach, which can be easily implemented in routine breast MRI exams, aids in the non-invasive identification of aggressive breast cancer.
Primary Thromboprophylaxis in Ambulatory Pancreatic Cancer Patients Receiving Chemotherapy: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Frere Corinne , Crichi Benjamin , Bournet Barbara , Canivet Cindy , Abdallah Nassim Ait , Buscail Louis , Farge Dominique
Published: July 2020

Patients with pancreatic cancer (PC) carry the highest risk of venous thromboembolism (VTE) amongst all cancer patients. Appropriate use of primary thromboprophylaxis might significantly and safely reduce its burden. We performed a systematic review of published studies and meeting abstracts using MEDLINE and EMBASE through July 2020 to evaluate the efficacy and safety of primary thromboprophylaxis in ambulatory PC patients receiving chemotherapy. The Mantel–Haenszel random effect model was used to estimate the pooled event-based risk ratio (RR) and the pooled absolute risk difference (RD) with a 95% confidence interval (CI). Five randomized controlled studies with 1003 PC patients were included in this meta-analysis. Compared to placebo, thromboprophylaxis significantly decreased the risk of VTE (pooled RR 0.31, 95% CI 0.19–0.51, p < 0.00001, I2 = 8%; absolute RD −0.08, 95% CI −0.12–−0.05, p < 0.00001, I2 = 0%), with an estimated number needed to treat of 11.9 patients to prevent one VTE event. Similar reductions of VTE were observed in studies with parenteral (RR 0.30, 95% CI 0.17–0.53) versus oral anticoagulants (RR 0.37, 95% CI 0.14–0.99) and in studies using prophylactic doses of anticoagulants (RR 0.34, 95% CI 0.17–0.70) versus supra-prophylactic doses of anticoagulants (RR 0.27, 95% CI 0.08–0.90). The pooled RR for major bleeding was 1.08 (95% CI 0.47–2.52, p = 0.85, I2 = 0%) and the absolute RD was 0.00 (95% CI −0.02–0.03, p = 0.85, I2 = 0%). Evidence supports a net clinical benefit of thromboprophylaxis in ambulatory PC patients receiving chemotherapy. Adequately powered randomized phase III studies assessing the most effective anticoagulant and the optimal dose, schedule and duration of thromboprophylaxis to be used are warranted.
Colorectal Cancer and Bone Tissue: Fantastic Relations and Where to Find Them
Authors: Gigante Isabella , Tutino Valeria , De Nunzio Valentina , Notarnicola Maria
Published: July 2020

Colorectal cancer (CRC) is the third most common cancer worldwide. There is a need for the early diagnosis of CRC for a better prognostic outcome. It is, therefore, crucial to understand the CRC pathogenesis in all its aspects. In many cases, one of the main causes of cancer-related deaths is the presence of metastases. In this context, an often overlooked aspect is the metastatic tropism, since CRC, like other cancers, is more prone to metastasize some organs rather than others. Beyond the liver and lung, and differently from other types of cancers, a not usual site of CRC metastases is the bone. However, it may assume a crucial role in the development and the outcome of the disease. Therefore, this review aims to discuss the complex relations between bone markers and CRC pathogenesis, suggesting the use of these molecules as potential targets for therapeutic purposes. Different osteogenic molecules, some of whom are growth factors and are implicated in the different osteogenic pathways, have been proved to also be involved in CRC progression. Some of them are oncogenes, while others oncosuppressors, and in a future perspective, some of them may represent new potential CRC biomarkers.
Histopathological Classification of Breast Cancer Images Using a Multi-Scale Input and Multi-Feature Network
Authors: Sheikh Taimoor Shakeel , Lee Yonghee , Cho Migyung
Published: July 2020

Diagnosis of pathologies using histopathological images can be time-consuming when many images with different magnification levels need to be analyzed. State-of-the-art computer vision and machine learning methods can help automate the diagnostic pathology workflow and thus reduce the analysis time. Automated systems can also be more efficient and accurate, and can increase the objectivity of diagnosis by reducing operator variability. We propose a multi-scale input and multi-feature network (MSI-MFNet) model, which can learn the overall structures and texture features of different scale tissues by fusing multi-resolution hierarchical feature maps from the network’s dense connectivity structure. The MSI-MFNet predicts the probability of a disease on the patch and image levels. We evaluated the performance of our proposed model on two public benchmark datasets. Furthermore, through ablation studies of the model, we found that multi-scale input and multi-feature maps play an important role in improving the performance of the model. Our proposed model outperformed the existing state-of-the-art models by demonstrating better accuracy, sensitivity, and specificity.
Transcriptomic Profile of Lymphovascular Invasion, a Known Risk Factor of Pancreatic Ductal Adenocarcinoma Metastasis
Authors: Takahashi Hideo , Katsuta Eriko , Yan Li , Tokumaru Yoshihisa , Katz Matthew H.G. , Takabe Kazuaki
Published: July 2020

Lymphovascular invasion (LVI) is an aggressive pathologic feature and considered a risk factor for distant metastasis. We hypothesized that pancreatic ductal adenocarcinomas (PDACs) with LVI are associated with shorter survival, as well as aggressive cancer biology and lymphangiogenesis in transcriptomic analysis. Utilizing the cancer genome atlas (TCGA)-PDAC cohort, we found that positive LVI was significantly associated with positive perineural invasion (PNI) (p = 0.023), and higher American Joint Committee on Cancer (AJCC) T (p = 0.017) and N (p < 0.001) categories. Furthermore, positive LVI was associated with shorter overall survival (OS) (p = 0.014) and was an independent risk factor of poor OS. Although there was no association between LVI status and lymphangiogenesis, epithelial-mesenchymal transition (EMT), or metastasis-related genes, Gene Set Enrichment Analysis revealed a strong association with cell-proliferation-related gene sets such as mitotic spindles (Normalized enrichment score (NES) = 1.76, p = 0.016) and G2/M checkpoints (NES = 1.75, p = 0.036), as well as with transforming growth factor beta (TGF-beta) signaling (NES = 1.61, p = 0.043), which is a known mechanism of metastasis in PDACs. In conclusion, positive LVI was an independent risk factor of poor OS in PDACs. We found that PDACs with LVI were possibly associated with accelerated cell proliferation and enhanced TGF-beta signaling independent of lymphangiogenesis. Transcriptomic profiling elucidates more precise tumor biology of LVI-positive PDACs.
Emerging Contribution of PancRNAs in Cancer
Authors: Mercatelli Neri , Palombo Ramona , Paronetto Maria Paola
Published: July 2020

“Cancer” includes a heterogeneous group of diseases characterized by abnormal growth beyond natural boundaries. Neoplastic transformation of cells is orchestrated by multiple molecular players, including oncogenic transcription factors, epigenetic modifiers, RNA binding proteins, and coding and noncoding transcripts. The use of computational methods for global and quantitative analysis of RNA processing regulation provides new insights into the genomic and epigenomic features of the cancer transcriptome. In particular, noncoding RNAs are emerging as key molecular players in oncogenesis. Among them, the promoter-associated noncoding RNAs (pancRNAs) are noncoding transcripts acting in cis to regulate their host genes, including tumor suppressors and oncogenes. In this review, we will illustrate the role played by pancRNAs in cancer biology and will discuss the latest findings that connect pancRNAs with cancer risk and progression. The molecular mechanisms involved in the function of pancRNAs may open the path to novel therapeutic opportunities, thus expanding the repertoire of targets to be tested as anticancer agents in the near future.
Friend or Foe: S100 Proteins in Cancer
Authors: Allgöwer Chantal , Kretz Anna-Laura , von Karstedt Silvia , Wittau Mathias , Henne-Bruns Doris , Lemke Johannes
Published: July 2020

S100 proteins are widely expressed small molecular EF-hand calcium-binding proteins of vertebrates, which are involved in numerous cellular processes, such as Ca2+ homeostasis, proliferation, apoptosis, differentiation, and inflammation. Although the complex network of S100 signalling is by far not fully deciphered, several S100 family members could be linked to a variety of diseases, such as inflammatory disorders, neurological diseases, and also cancer. The research of the past decades revealed that S100 proteins play a crucial role in the development and progression of many cancer types, such as breast cancer, lung cancer, and melanoma. Hence, S100 family members have also been shown to be promising diagnostic markers and possible novel targets for therapy. However, the current knowledge of S100 proteins is limited and more attention to this unique group of proteins is needed. Therefore, this review article summarises S100 proteins and their relation in different cancer types, while also providing an overview of novel therapeutic strategies for targeting S100 proteins for cancer treatment.
Ultra-Short Circulating Tumor DNA (usctDNA) in Plasma and Saliva of Non-Small Cell Lung Cancer (NSCLC) Patients
Authors: Li Feng , Wei Fang , Huang Wei-Lun , Lin Chien-Chung , Li Liang , Shen Macy M. , Yan Qingxiang , Liao Wei , Chia David , Tu Michael , Tang Jason H. , Feng Ziding , Kim Yong , Su Wu-Chou , Wong David T. W.
Published: July 2020

Mutations identified in the epidermal growth factor receptor (EGFR) predict sensitivity to EGFR-targeted therapy for non-small cell lung carcinoma (NSCLC). We previously reported that Electric Field-Induced Release and Measurement (EFIRM)-based liquid biopsy could detect EGFR ctDNA with >94% concordance with tissue-based genotyping. A side-by-side comparison of concordance of EFIRM and droplet digital PCR (ddPCR) for the detection of the two front-line actionable EFGR mutations was performed with paired plasma and saliva samples from 13 NSCLC patients. Deep sequencing analysis based on single-strand DNA library preparation was employed to determine the size distributions of EGFR L858R ctDNA in plasma and saliva samples. EFIRM detected both EGFR mutations with 100% sensitivity in both plasma and saliva samples, whereas ddPCR detected EGFR mutations with sensitivities of 84.6% and 15.4%, respectively. In saliva samples, the majority of EGFR L858R ctDNA fragments detected were <80 bp. Deep sequencing analysis of ctDNA enriched for the EGFR L858R mutation revealed the significant presence of EGFR L858R ctDNA as ultra-short circulating tumor DNA (usctDNA) with the size of 40–60 bp in patient plasma and saliva. Most of usctDNAs are not amplifiable with the current ddPCR assay. Further examination using cell lines and patient biofluids revealed that the majority of usctDNAs were predominately localized in the exosomal fraction. Our study revealed the abundant existence of EGFR ctDNA in the plasma and saliva of NSCLC patients is usctDNA. usctDNA is a novel type of targets for liquid biopsy that can be efficiently detected by EFIRM technology.
Real-World Outcomes of Patients with Refractory or Relapsed FLT3-ITD Acute Myeloid Leukemia: A Toulouse-Bordeaux DATAML Registry Study
Authors: Dumas Pierre-Yves , Bertoli Sarah , Bérard Emilie , Largeaud Laetitia , Bidet Audrey , Delabesse Eric , Leguay Thibaut , Leroy Harmony , Gadaud Noémie , Rieu Jean Baptiste , Vial Jean-Philippe , Vergez François , Lechevalier Nicolas , Luquet Isabelle , Klein Emilie , Sarry Audrey , de Grande Anne-Charlotte , Pigneux Arnaud , Récher Christian
Published: July 2020

Two recent phase 3 trials showed that outcomes for relapsed/refractory (R/R) FLT3-mutated acute myeloid leukemia (AML) patients may be improved by a single-agent tyrosine kinase inhibitor (TKI) (i.e., quizartinib or gilteritinib). In the current study, we retrospectively investigated the characteristics and real-world outcomes of R/R FLT3-internal tandem duplication (ITD) acute myeloid leukemia (AML) patients in the Toulouse-Bordeaux DATAML registry. In the study, we included 316 patients with FLT3-ITD AML that received intensive chemotherapy as a first-line treatment. The rate of complete remission (CR) or CR without hematological recovery (CRi) was 75.2%, and 160 patients were R/R after a first-line TKI-free treatment (n = 294). Within the subgroup of R/R patients that fulfilled the main criteria of the QUANTUM-R study, 48.9% received an intensive salvage regimen; none received hypomethylating agents or low-dose cytarabine. Among the R/R FLT3-ITD AML patients with CR1 durations < 6 months who received intensive TKI-free treatment, the rate of CR or CRi after salvage chemotherapy was 52.8%, and these results allowed a bridge to be transplanted in 39.6% of cases. Finally, in this QUANTUM-R standard arm-matched cohort, the median overall survival (OS) was 7.0 months and 1-, 3- and 5-year OS were 30.2%, 23.7% and 21.4%, respectively. To conclude, these real-world data show that the intensity of the second-line treatment likely affects response and transplantation rates. Furthermore, the results indicate that including patients with low-intensity regimens, such as low-dose cytarabine or hypomethylating agents, in the control arm of a phase 3 trial may be counterproductive and could compromise the results of the study.
Application of Enzymes in Regioselective and Stereoselective Organic Reactions
Authors: Mu Ruipu , Wang Zhaoshuai , Wamsley Max C. , Duke Colbee N. , Lii Payton H. , Epley Sarah E. , Todd London C. , Roberts Patty J.
Published: July 2020

Nowadays, biocatalysts have received much more attention in chemistry regarding their potential to enable high efficiency, high yield, and eco-friendly processes for a myriad of applications. Nature’s vast repository of catalysts has inspired synthetic chemists. Furthermore, the revolutionary technologies in bioengineering have provided the fast discovery and evolution of enzymes that empower chemical synthesis. This article attempts to deliver a comprehensive overview of the last two decades of investigation into enzymatic reactions and highlights the effective performance progress of bio-enzymes exploited in organic synthesis. Based on the types of enzymatic reactions and enzyme commission (E.C.) numbers, the enzymes discussed in the article are classified into oxidoreductases, transferases, hydrolases, and lyases. These applications should provide us with some insight into enzyme design strategies and molecular mechanisms.
Prediction of a Stable Organic Metal-Free Porous Material as a Catalyst for Water-Splitting
Authors: Li Hengshuai , Hu Haiquan , Bai Chenglin , Bao Chunjiang , Liu Cailong , Wang Qinglin , Guo Feng , Feng Zhenbao , Yu Hanwen , Chen Ming , Qu Konggang
Published: July 2020

It is of practical significance to find organic metal-free catalyst materials. We propose a new graphene-like carbon nitride structure, which was able to meet these requirements well. Its primitive cell consists of eight carbon atoms and six nitrogen atoms. Hence, we called this structure g–C8N6. The stability of the structure was verified by phonon spectroscopy and molecular dynamics simulations. Then its electronic structure was calculated, and its band edge position was compared with the redox potential of water. We analyzed its optical properties and electron–hole recombination rate. After the above analysis, it is predicted that it is a suitable photocatalyst material. To improve its photocatalytic performance, two methods were proposed: applied tensile force and multilayer stacking. Our research is instructive for the photocatalytic application of this kind of materials.
Synthesis of Biogenic Palladium Nanoparticles Using Citrobacter sp. for Application as Anode Electrocatalyst in a Microbial Fuel Cell
Authors: Matsena Mpumelelo Thomas , Tichapondwa Shepherd Masimba , Chirwa Evans Martin Nkhalambayausi
Published: July 2020

Palladium (Pd) is a cheap and effective electrocatalyst that is capable of replacing platinum (Pt) in various applications. However, the problem in using chemically synthesized Pd nanoparticles (PdNPs) is that they are mostly fabricated using toxic chemicals under severe conditions. In this study, we present a more environmentally-friendly process in fabricating biogenic Pd nanoparticles (Bio-PdNPs) using Citrobacter sp. isolated from wastewater sludge. Successful fabrication of Bio-PdNPs was achieved under anaerobic conditions at pH six and a temperature of 30 °C using sodium formate (HCOONa) as an electron donor. Citrobacter sp. showed biosorption capabilities with no enzymatic contribution to Pd(II) uptake during absence of HCOONa in both live and dead cells. Citrobacter sp. live cells also displayed high enzymatic contribution to the removal of Pd(II) by biological reduction. This was confirmed by Scanning Electron Microscope (SEM), Electron Dispersive Spectroscopy (EDS), and X-ray Diffraction (XRD) characterization, which revealed the presence Bio-PdNPs deposited on the bacterial cells. The bio-PdNPs successfully enhanced the anode performance of the Microbial Fuel Cell (MFC). The MFC with the highest Bio-PdNPs loading (4 mg Bio-PdNP/cm2) achieved a maximum power density of 539.3 mW/m3 (4.01 mW/m2) and peak voltage of 328.4 mV.
High-Complexity WO3-Based Catalyst with Multi-Catalytic Species via 3D Printing
Authors: Wang Xiaofeng , Guo Wei , Abu-Reziq Raed , Magdassi Shlomo
Published: July 2020

Three-dimensional (3D) printing has recently been introduced into the field of chemistry as an enabling tool employed to perform reactions, but so far, its use has been limited due to material and structural constraints. We have developed a new approach for fabricating 3D catalysts with high-complexity features for chemical reactions via digital light processing printing (DLP). PtO2-WO3 heterogeneous catalysts with complex shapes were directly fabricated from a clear solution, composed of photo-curable organic monomers, photoinitiators, and metallic salts. The 3D-printed catalysts were tested for the hydrogenation of alkynes and nitrobenzene, and displayed excellent reactivity in these catalytic transformations. Furthermore, to demonstrate the versatility of this approach and prove the concept of multifunctional reactors, a tungsten oxide-based tube consisting of three orderly sections containing platinum, rhodium, and palladium was 3D printed.
Deepening the Mechanisms of Visceral Pain Persistence: An Evaluation of the Gut-Spinal Cord Relationship
Authors: Lucarini Elena , Parisio Carmen , Branca Jacopo J. V. , Segnani Cristina , Ippolito Chiara , Pellegrini Carolina , Antonioli Luca , Fornai Matteo , Micheli Laura , Pacini Alessandra , Bernardini Nunzia , Blandizzi Corrado , Ghelardini Carla , Di Cesare Mannelli Lorenzo
Published: July 2020

The management of visceral pain is a major clinical problem in patients affected by gastrointestinal disorders. The poor knowledge about pain chronicization mechanisms prompted us to study the functional and morphological alterations of the gut and nervous system in the animal model of persistent visceral pain caused by 2,4-dinitrobenzenesulfonic acid (DNBS). This agent, injected intrarectally, induced a colonic inflammation peaking on day 3 and remitting progressively from day 7. In concomitance with bowel inflammation, the animals developed visceral hypersensitivity, which persisted after colitis remission for up to three months. On day 14, the administration of pain-relieving drugs (injected intraperitoneally and intrathecally) revealed a mixed nociceptive, inflammatory and neuropathic pain originating from both the peripheral and central nervous system. At this time point, the colonic histological analysis highlighted a partial restitution of the tunica mucosa, transmural collagen deposition, infiltration of mast cells and eosinophils, and upregulation of substance P (SP)-positive nerve fibers, which were surrounded by eosinophils and MHC-II-positive macrophages. A significant activation of microglia and astrocytes was observed in the dorsal and ventral horns of spinal cord. These results suggest that the persistence of visceral pain induced by colitis results from maladaptive plasticity of the enteric, peripheral and central nervous systems.
Implications of Insulin-Like Growth Factor-1 in Skeletal Muscle and Various Diseases
Authors: Ahmad Syed Sayeed , Ahmad Khurshid , Lee Eun Ju , Lee Yong-Ho , Choi Inho
Published: July 2020

Skeletal muscle is an essential tissue that attaches to bones and facilitates body movements. Insulin-like growth factor-1 (IGF-1) is a hormone found in blood that plays an important role in skeletal myogenesis and is importantly associated with muscle mass entity, strength development, and degeneration and increases the proliferative capacity of muscle satellite cells (MSCs). IGF-1R is an IGF-1 receptor with a transmembrane location that activates PI3K/Akt signaling and possesses tyrosine kinase activity, and its expression is significant in terms of myoblast proliferation and normal muscle mass maintenance. IGF-1 synthesis is elevated in MSCs of injured muscles and stimulates MSCs proliferation and myogenic differentiation. Mechanical loading also affects skeletal muscle production by IGF-1, and low IGF-1 levels are associated with low handgrip strength and poor physical performance. IGF-1 is potentially useful in the management of Duchenne muscular dystrophy, muscle atrophy, and promotes neurite development. This review highlights the role of IGF-1 in skeletal muscle, its importance during myogenesis, and its involvement in different disease conditions.
Microplasma-Sprayed V2O5/C Double-Layer Coating for the Parts of Mini-Hydropower Systems
Authors: Zhilkashinova Almira , Abilev Madi , Zhilkashinova Assel
Published: July 2020

The development of novel designs for hydropower plants is of high interest nowadays. Studies have shown the negative effect of fluid flow on the turbines of mini-hydropower plants when using them in the conditions of the mountain river. To reduce the damage caused by cavitation, a microplasma coating technique has been chosen. Due to its wetting ability, low density, high thermal conductivity, high heat resistance and low chemical activity, graphite has been studied as a coating material. Vanadium pentoxide has been added as an interlayer to increase the wear resistance, corrosion resistance, and adhesion of the system. The microstructure of the system was studied using scanning electron microscopy and transmission electron microscopy. Functional properties of the system were tested by microhardness tests, wear resistance tests (friction), corrosion tests, and pull-off tests. The surface of the coating was homogeneous without warping, swelling and cracking. The microstructure consisted of regular structures in the form of branches of dendrites. V2O5/C coating resulted in the increase in microhardness up to 2534 MPa. The wear resistance (volume loss) of the sample with double-layer coating was 0.14 mm3 and the maximum adhesion strength was 17.5 MPa. Thus, the double-layer microplasma V2O5/C coating was applied and studied for strengthening the blades of mini-HPP. The microplasma method can find application in modifying the surface of power equipment subjected to the cavitation effect of the river water.
Effects of the Size of Charged Nanoparticles on the Crystallinity of SiC Films Prepared by Hot Wire Chemical Vapor Deposition
Authors: Kim Daseul , Kim Du-Yun , Kwon Ji-Hye , Hwang Nong-Moon
Published: July 2020

Non-classical crystallization suggests that crystals can grow with nanoparticles as a building block. In this case, the crystallization behavior depends on the size and charge of the nanoparticles. If charged nanoparticles (CNPs) are small enough, they become liquid-like and tend to undergo epitaxial recrystallization. Here, the size effect of SiC CNPs on film crystallinity was studied in the hot-wire chemical vapor deposition process. To do this, SiC nanoparticles were captured under different processing conditions—in this case, wire temperature, precursor concentration and the filament bias. Increasing the temperature of tungsten wires and decreasing the ratio of (SiH4 + CH4)/H2 reduced the size of the SiC nanoparticles. When the nanoparticles were small enough, an epitaxial SiC film approximately 100-nm-thick was grown, whereas larger nanoparticles produced polycrystalline SiC films. These results suggest that the size of the CNPs is an important process variable when growing films by means of non-classical crystallization.
Artificial Ageing of Photocatalytic Nanocomposites for the Protection of Natural Stones
Authors: Roveri Marco , Goidanich Sara , Toniolo Lucia
Published: July 2020

During the last ten years, photocatalytic nanocomposites combining titania nanoparticles with silicon-based matrices have received increasing attention in the stone conservation research field, because they offer an effective multifunctional approach to the issue of stone protection. However, much work still has to be done in studying the behaviour of these nanocomposites in real environmental conditions and understanding to what extent they are able to retain their effectiveness and compatibility once applied on outdoor surfaces. The latter is a key information that should lie at the basis of any successful conservation and maintenance campaign. The present study provides insight into this relevant topic trough laboratory testing by assessing the artificial ageing of two silane-based photocatalytic nanocomposites, previously selected through an accurate testing on different natural stones. Three accelerated ageing procedures, based on artificial solar irradiation, heating and rain wash-out, allowed simulating about two years of outdoor exposure to some of the weathering factors to which stones are normally subjected. The results provided quite accurate information about the long-term behaviour of the products and on the role that the stone properties play therein. It was shown that, when the products are able to penetrate deeply enough inside the stone pores, they retain much of their hydrophobising and photocatalytic properties and maintain a good compatibility with the stone substrates, even after partial chemical degradation of the alkyl-silica matrices has occurred on the very stone surface.
Edible Coatings Formulated with Antifungal GRAS Salts to Control Citrus Anthracnose Caused by Colletotrichum gloeosporioides and Preserve Postharvest Fruit Quality
Authors: Martínez-Blay Victoria , Pérez-Gago María B. , de la Fuente Beatriz , Carbó Rosario , Palou Lluís
Published: July 2020

The in vitro antifungal activity of various generally recognized as safe (GRAS) salts against Colletotrichum gloeosporioides, the causal agent of citrus postharvest anthracnose, was evaluated as mycelial growth reduction on potato dextrose agar (PDA) dishes amended with salt aqueous solutions at different concentrations. The most effective treatments [0.2% ammonium carbonate (AC), 2% potassium sorbate (PS), 0.2% potassium carbonate (PC), 0.1% sodium methylparaben (SMP), 0.1% sodium ethylparaben (SEP), 2% sodium benzoate (SB) and 2% potassium silicate (PSi)] were selected as antifungal ingredients of composite edible coatings formulated with hydroxypropyl methylcellulose (HPMC)-beeswax (BW) matrixes. Stable coatings containing these salts were applied in in vivo curative experiments to “Nadorcott” mandarins and “Valencia” oranges artificially inoculated with C. gloeosporioides and those containing 2% PS, 2% SB and 2% PSi were the most effective to reduce anthracnose severity with respect to control fruit (up to 70% on mandarins). The effect of these selected coatings on the quality of non-inoculated and cold-stored “Valencia” oranges was determined after 28 and 56 days at 5 °C and 90% RH, followed by 7 days of shelf life at 20 °C. None of the coatings significantly reduced weight loss of coated oranges, but they modified their internal atmosphere, increasing the CO2 content. Overall, the coatings did not adversely affect the physicochemical and sensory attributes of the fruit.
Phenomenon of Electromagnetic Field Resonance in Metal and Dielectric Gratings and Its Possible Practical Applications
Authors: Bellucci Stefano , Bendziak Andrii , Vernyhor Oleksandr , Fitio Volodymyr M.
Published: July 2020

Calculations of the field distribution in the structure of the dielectric substrate/buffer layer/volume phase grating/analyzed medium were performed. It is shown that in the presence of a buffer layer with a low refractive index in the dielectric waveguide leads to a shift of the maximum field at the waveguide resonance into analyzed medium. As a result, the spectral and angular sensitivity of the corresponding sensor increases. Based on the waveguide equation, analytical expressions are obtained that connect the spectral and angular sensitivity of the sensor to the sensitivity of the propagation constant change due to the refractive index change of the analyzed medium. The conditions for the excitation of the resonance of surface plasmon–polariton waves in the structure with a metal or dielectric grating on a metal substrate are also given. The fields that occur at resonance for silver and gold gratings are calculated.
Comparative Analysis of the Core Proteomes among the Pseudomonas Major Evolutionary Groups Reveals Species-Specific Adaptations for Pseudomonas aeruginosa and Pseudomonas chlororaphis
Authors: Nikolaidis Marios , Mossialos Dimitris , Oliver Stephen G. , Amoutzias Grigorios D.
Published: July 2020

The Pseudomonas genus includes many species living in diverse environments and hosts. It is important to understand which are the major evolutionary groups and what are the genomic/proteomic components they have in common or are unique. Towards this goal, we analyzed 494 complete Pseudomonas proteomes and identified 297 core-orthologues. The subsequent phylogenomic analysis revealed two well-defined species (Pseudomonas aeruginosa and Pseudomonas chlororaphis) and four wider phylogenetic groups (Pseudomonas fluorescens, Pseudomonas stutzeri, Pseudomonas syringae, Pseudomonas putida) with a sufficient number of proteomes. As expected, the genus-level core proteome was highly enriched for proteins involved in metabolism, translation, and transcription. In addition, between 39–70% of the core proteins in each group had a significant presence in each of all the other groups. Group-specific core proteins were also identified, with P. aeruginosa having the highest number of these and P. fluorescens having none. We identified several P. aeruginosa-specific core proteins (such as CntL, CntM, PlcB, Acp1, MucE, SrfA, Tse1, Tsi2, Tse3, and EsrC) that are known to play an important role in its pathogenicity. Finally, a holin family bacteriocin and a mitomycin-like biosynthetic protein were found to be core-specific for P. cholororaphis and we hypothesize that these proteins may confer a competitive advantage against other root-colonizers.
Efficient Quantum Simulation of an Anti-P-Pseudo-Hermitian Two-Level System
Authors: Zheng Chao , Tian Jin , Li Daili , Wen Jingwei , Wei Shijie , Li Yansong
Published: July 2020

Besides Hermitian systems, quantum simulation has become a strong tool to investigate non-Hermitian systems, such as PT-symmetric, anti-PT-symmetric, and pseudo-Hermitian systems. In this work, we theoretically investigate quantum simulation of an anti-P-pseudo-Hermitian two-level system in different dimensional Hilbert spaces. In an arbitrary phase, we find that six dimensions are the minimum to construct the anti-P-pseudo-Hermitian two-level subsystem, and it has a higher success probability than using eight dimensions. We find that the dimensions can be reduced further to four or two when the system is in the anti-PT-symmetric or Hermitian phase, respectively. Both qubit-qudit hybrid and pure-qubit systems are able to realize the simulation, enabling experimental implementations in the near future.
Efficient Electricity Management System for Optimal Peak/Off-Peak Hour Pricing
Authors: Bandari Naresh , Raghavendra Kummara Venkata Guru , Heo Sujin , Kumar Seelam Vasavi Sai Viswanada Prabhu Deva , Uddin Waqar , Sama Lenin Kumar Reddy , Punnoose Dinah , Kim Hee-Je
Published: July 2020

With the advent of new technologies and an alarming increase in the world’s population, there has been a rapid increase in energy consumption. Consequently, this has resulted in a surge in developing sources that generate electricity and concurrently escalating global warming levels. Owing to their contributions in vast applications, dependence on renewable energy is a reliable option. However, it is known that a complete and efficient utilization of the incoming solar radiation is not feasible, taking into account the various losses associated. Our proposal addresses concerns resulting in the efficient utilization of solar energy based on optimal cost analysis by the mathematical procedure. This methodology when used along with a battery-based photovoltaic (PV) system effectively reduces the amount of electricity imported from the grid. The implementation of this method scales down the monthly electricity consumption by 67.1%. Our findings were established considering South Korea’s residential electricity tariff system. Our system works based on a principle where the batteries are charged with solar PV during off-peak hours and discharged during peak hours. The state of charge of the battery could be monitored using a web server. In situations, wherein the load demand cannot be sustained by the batteries, grid power can be utilized during peak hours. The sequence of these events can be implemented by a series of algorithms. Our proposed system also helps in achieving the goal-7 of the sustainable development goals (SDG) prescribed by the United Nations (UN), which is to boost the consumption of renewable energy which ultimately results in monetary savings to a large extent.
A Non-WSSUS Channel Simulator for V2X Communication Systems
Authors: Jaime-Rodríguez José Jimmy , Gómez-Vega Carlos Antonio , Gutiérrez Carlos A. , Luna-Rivera José Martín , Campos-Delgado Daniel Ulises , Velázquez Ramiro
Published: July 2020

This paper presents a simulator of non-wide sense stationary uncorrelated scattering (non-WSSUS) multipath fading channels for the performance analysis of vehicle-to-everything (V2X) communication systems. The proposed simulator is constructed with the combination of the Monte Carlo and sum-of-cisoids (SOC) principles, and it is suitable for multicarrier transmission schemes such as those defined for dedicated short-range communications (DSRC) and cellular-based V2X (C-V2X) communications. The channel simulator provides an accurate and flexible solution to reproduce the time and frequency (TF) correlation properties of non-WSSUS vehicular channels under arbitrary isotropic and non-isotropic scattering conditions. Furthermore, the proposed simulator allows velocity variations and non-linear trajectories of the mobile stations (MSs). To demonstrate the practical value of the presented simulator, we evaluate the bit error rate (BER) performance of two channel estimation techniques that are considered for IEEE 802.11p transceivers, namely the least squares (LS) estimator and the spectral temporal averaging (STA) technique. The BER performance of both channel estimators was analyzed by considering three propagating scenarios for road safety applications. Our results show that the non-stationary characteristics of the vehicular multipath fading channel have nearly no effects on the LS estimator’s BER performance. In contrast, the performance of the STA estimator is significantly affected by the channel’s non-stationary characteristics. A variation of the original STA technique that applies only a temporal averaging is introduced in this work to improve the system’s BER in non-WSSUS channels.
Passive Round-Trip-Time Positioning in Dense IEEE 802.11 Networks
Authors: Martin-Escalona Israel , Zola Enrica
Published: July 2020

The search for a unique and globally available location solution has attracted researchers for a long time. However, a solution for indoor scenarios, where high accuracy is needed, and Global Positioning System (GPS) is not available, has not been found yet. Despite the number of proposals in the literature, some require too long a calibration time for constructing the fingerprinting map, some rely on the periodic broadcast of positioning information that may downgrade the data communication channel, while others require specific hardware components that are not expected to be carried on commercial off-the-shelf (COTS) wireless devices. The scalability of the location solution is another key parameter for next-generation internet of things (IoT) and 5G scenarios. A passive solution for indoor positioning of WiFi devices is first introduced here, which merges a time-difference of arrival (TDOA) algorithm with the novel fine time measurements (FTM) introduced in IEEE 802.11mc. A proof of concept of the WiFi passive TDOA algorithm is detailed in this paper, together with a thorough discussion on the requirements of the proposed algorithm.
New Design of Copper–Inconel 601 Ground Electrode Spark Plug Based on a Thermo-Mechanical Model
Authors: Tahri Chawki , Klocker Helmut , Beaugiraud Bernard , Bertoni Christophe , Feulvarch Eric , Bergheau Jean-Michel
Published: July 2020

Inconel 601 is one material of choice for intermediate- to high-temperature protective coatings for spark plugs’ ground electrodes. Production of ground electrodes of spark plugs implies the following operations: the tamping of the copper core in an Inconel 601 cup, cold-forming of the assembly, annealing, welding, and bending of the final spark plug. On the production line, the use of Inconel 601 as a protective coating for ground electrodes leads to possible cracking in the welded area after bending. In the present paper, possible causes of cracking are analyzed. It is clearly shown that a combination of Copper –Inconel interface oxidation, Inconel yielding during the heat treatment, and micro-movements during bending lead to cracks in the welded area of the ground electrode. First, the detrimental effect of gaps, between Copper and Inconel 601, is shown experimentally. Second, a thermo-mechanical analysis combined with SEM (Scanning Electron Microscopy) observations identified the annealing treatment and interface oxidation as the main cause of gaps. Third, bending simulations show the relation between these gaps and cracking. Finally, a new ground electrode design, preventing cracks, is suggested.
Direct Form Digital Robust RST Control Based on Chebyshev Sphere Optimization Applied in a DC-DC Power Converter
Authors: C. das Neves Cleonor , B. Junior Walter , Medeiros Renan L. P. de , Ayres Junior Florindo A. C. , V. Bessa Iury , Bessa Isaías V. , de M. Veroneze Gabriela , E. S. e Silva Luiz , J. S. Farias Nei
Published: July 2020

This paper presents a novel direct form to design a digital robust control using RST structure (i.e., name given because of the R, S and T polynomials computed) based on convex optimization such as Chebyshev sphere; this approach was applied to a DC-DC Buck converter. This methodology takes into account parametric uncertainties and a Chebyshev sphere constraint in order to ensure robust performance and stability of the system in the discrete domain. For this purpose, a mathematical model for the DC-DC Buck converter is presented when considering uncertainties in electrical variables, such as load resistance, inductance, capacitance, and source voltage variation, also to obtain the discrete model of the system by using the bilinear transformation. The proposed methodology is compared with two other approaches designed in a discrete domain: the classical pole placement and the robust methodology based on the Kharitonov theorem. Wide-ranging experiments are performed in order to evaluate the behavior of the control methodologies when the system is subject to parametric variations of the load resistance and voltage setpoint variation. The results show that the proposed methodology outperforms the other approaches in 90% of the tests and ensures robust stability and robust performance when the system is subjected to a parametric uncertainties family.
Degradation of Hydrophobic, Anti-Soiling Coatings for Solar Module Cover Glass
Authors: Lisco Fabiana , Bukhari Farwah , Uličná Soňa , Isbilir Kenan , Barth Kurt L. , Taylor Alan , Walls John M.
Published: July 2020

Soiling of solar module cover glass is a serious problem for solar asset managers. It causes a reduction in power output due to attenuation of the incident light, and reduces the return on investment. Regular cleaning is required to mitigate the effect but this is a costly procedure. The application of transparent hydrophobic, anti-soiling coatings to the cover glass is a promising solution. These coatings have low surface energy and contaminants do not adhere well. Even if soiling does remain on the coated surface, it is much more easily removed during cleaning. The performance of the coatings is determined using the water contact angle and roll-off angle measurements. However, although hydrophobic coatings hold out great promise, outdoor testing revealed degradation that occurs surprisingly quickly. In this study, we report on results using laboratory-based damp heat and UV exposure environmental tests. We used SEM surface imaging and XPS surface chemical analysis to study the mechanisms that lead to coating degradation. Loss of surface fluorine from the coatings was observed and this appeared to be a major issue. Loss of nanoparticles was also observed. Blistering of surfaces also occurs, leading to loss of coating material. This was probably due to the movement of retained solvents and was caused by insufficient curing. This mechanism is avoidable if care is taken for providing and carrying out carefully specified curing conditions. All these symptoms correlate well with observations taken from parallel outdoor testing. Identification of the mechanisms involved will inform the development of more durable anti-soiling, hydrophobic coatings for solar application.