bluuebunny/crossref_metadata_2025 huggingface dataset:
Acetabular complications are the most common cause for revision surgery following proximal femoral endoprosthetic replacement
Authors: Lex Johnathan Robert , Evans Scott , Parry Michael C. , Jeys Lee , Stevenson Jonathan D.
Published: September 2021

Aims

Proximal femoral endoprosthetic replacements (PFEPRs) are the most common reconstruction option for osseous defects following primary and metastatic tumour resection. This study aimed to compare the rate of implant failure between PFEPRs with monopolar and bipolar hemiarthroplasties and acetabular arthroplasties, and determine the optimum articulation for revision PFEPRs.

Methods

This is a retrospective review of 233 patients who underwent PFEPR. The mean age was 54.7 years (SD 18.2), and 99 (42.5%) were male. There were 90 patients with primary bone tumours (38.6%), 122 with metastatic bone disease (52.4%), and 21 with haematological malignancy (9.0%). A total of 128 patients had monopolar (54.9%), 74 had bipolar hemiarthroplasty heads (31.8%), and 31 underwent acetabular arthroplasty (13.3%).

Results

At a mean 74.4 months follow-up, the overall revision rate was 15.0%. Primary malignancy (p < 0.001) and age < 50 years (p < 0.001) were risk factors for revision. The risks of death and implant failure were similar in patients with primary disease (p = 0.872), but the risk of death was significantly greater for patients who had metastatic bone disease (p < 0.001). Acetabular-related implant failures comprised 74.3% of revisions; however, no difference between hemiarthroplasty or arthroplasty groups (p = 0.209), or between monopolar or bipolar hemiarthroplasties (p = 0.307), was observed. There was greater radiological wear in patients with longer follow-up and primary bone malignancy. Re-revision rates following a revision PFEPR was 34.3%, with dual-mobility bearings having the lowest rate of instability and re-revision (15.4%).

Conclusion

Hemiarthroplasty and arthroplasty PFEPRs carry the same risk of revision in the medium term, and is primarily due to acetabular complications. There is no difference in revision rates or erosion between monopolar and bipolar hemiarthroplasties. The main causes of failure were acetabular wear in the hemiarthroplasty group and instability in the arthroplasty group. These risks should be balanced and patient prognosis considered when contemplating the bearing choice. Dual-mobility, constrained bearings, or large diameter heads (> 32 mm) are recommended in all revision PFEPRs. Cite this article: Bone Joint J 2021;103-B(10):1633–1640.
Two-stage reconstruction of infected Charcot foot using internal fixation
Authors: Kavarthapu Venu , Budair Basil
Published: September 2021

Aims

In our unit, we adopt a two-stage surgical reconstruction approach using internal fixation for the management of infected Charcot foot deformity. We evaluate our experience with this functional limb salvage method.

Methods

We conducted a retrospective analysis of prospectively collected data of all patients with infected Charcot foot deformity who underwent two-stage reconstruction with internal fixation between July 2011 and November 2019, with a minimum of 12 months’ follow-up.

Results

We identified 23 feet in 22 patients with a mean age of 56.7 years (33 to 70). The mean postoperative follow-up period was 44.7 months (14 to 99). Limb salvage was achieved in all patients. At one-year follow-up, all ulcers have healed and independent full weightbearing mobilization was achieved in all but one patient. Seven patients developed new mechanical skin breakdown; all went on to heal following further interventions. Fusion of the hindfoot was achieved in 15 of 18 feet (83.3%). Midfoot fusion was achieved in nine of 15 patients (60%) and six had stable and painless fibrous nonunion. Hardware failure occurred in five feet, all with broken dorsomedial locking plate. Six patients required further surgery, two underwent revision surgery for infected nonunion, two for removal of metalwork and exostectomy, and two for dynamization of the hindfoot nail.

Conclusion

Two-stage reconstruction of the infected and deformed Charcot foot using internal fixation and following the principle of ‘long-segment, rigid and durable internal fixation, with optimal bone opposition and local antibiotic elusion’ is a good form of treatment provided a multidisciplinary care plan is delivered. Cite this article: Bone Joint J 2021;103-B(10):1611–1618.
Polychromatic annular folded lenses using freeform gradient-index optics
Authors: Lippman David H. , Chou Robert , Desai Ankur X. , Kochan Nicholas S. , Yang Tianyi , Schmidt Greg R. , Bentley Julie L. , Moore Duncan T.
Published: September 2021

The annular folded lens (AFL) is a design form offering large aperture, high-resolution imaging in a very axially compact package. The folded optic can be made monolithic for easier fabrication and alignment, yet the introduction of refractive surfaces with a dispersive optical material gives way to chromatic aberrations. AFL designs using homogeneous media are generally limited to the monochromatic regime with polychromatic performance greatly reduced. By introducing freeform gradient-index (F-GRIN) media, monolithic AFL designs can achieve higher monochromatic performance as well as provide color correction for diffraction-limited polychromatic imaging. Monochromatic and polychromatic design methodologies are surveyed where the F-GRIN is constrained to remain feasible for fabrication.
Combined anterior segment OCT and wavefront-based autorefractor using a shared beam
Authors: Ruggeri Marco , Belloni Giulia , Chang Yu-Cherng , Durkee Heather , Masetti Ettore , Cabot Florence , Yoo Sonia H. , Ho Arthur , Parel Jean-Marie , Manns Fabrice
Published: September 2021

We have combined an anterior segment (AS) optical coherence tomography (OCT) system and a wavefront-based aberrometer with an approach that senses ocular wavefront aberrations using the OCT beam. Temporal interlacing of the OCT and aberrometer channels allows for OCT images and refractive error measurements to be acquired continuously and in real-time. The system measures refractive error with accuracy and precision comparable to that of clinical autorefractors. The proposed approach provides a compact modular design that is suitable for integrating OCT and wavefront-based autorefraction within the optical head of the ophthalmic surgical microscope for guiding cataract surgery or table-top devices for simultaneous autorefraction and ocular biometry.
Simultaneous measurements of foveal and peripheral aberrations with accommodation in myopic and emmetropic eyes
Authors: Romashchenko Dmitry , Papadogiannis Petros , Unsbo Peter , Lundström Linda
Published: September 2021

The difference in peripheral retinal image quality between myopic and emmetropic eyes plays a major role in the design of the optical myopia interventions. Knowing this difference under accommodation can help to understand the limitations of the currently available optical solutions for myopia control. A newly developed dual-angle open-field sensor was used to assess the simultaneous foveal and peripheral (20

∘

nasal visual field) wavefront aberrations for five target vergences from -0.31 D to -4.0 D in six myopic and five emmetropic participants. With accommodation, the myopic eyes showed myopic shifts, and the emmetropic eyes showed no change in RPR. Furthermore, RPR calculated from simultaneous measurements showed lower intra-subject variability compared to the RPR calculated from peripheral measurements and target vergence. Other aberrations, as well as modulation transfer functions for natural pupils, were similar between the groups and the accommodation levels, foveally and peripherally. Results from viewing the same nearby target with and without spectacles by myopic participants suggest that the accommodative response is not the leading factor controlling the amplitude of accommodation microfluctuations.
Quantum limited timing jitter of soliton molecules in a mode-locked fiber laser
Authors: Zou Defeng , Li Zeqing , Qin Peng , Song Youjian , Hu Minglie
Published: September 2021

Soliton molecules in mode-locked lasers are expected to be ideal self-organization patterns, which warrant stability and robustness against perturbations. However, recent ultra-high resolution optical cross-correlation measurements uncover an intra-molecular timing jitter, even in stationary soliton molecules. In this work, we found that the intra-molecular timing jitter has a quantum origin. Numerical simulation indicates that amplified spontaneous emission (ASE) noise induces a random quantum diffusion for soliton pulse timing, which cannot be compensated by soliton binding mechanism. By suppressing indirectly coupled timing jitter at close-to-zero cavity dispersion, a record-low 350 as rms intra-soliton-molecular jittering is obtained from an Er-fiber laser in experiment. This work provides insight into the fundamental limits for the instability of multi-soliton patterns.
Improved shadow correction for the marine optical buoy, MOBY
Authors: Voss Kenneth , Leymarie Edouard , Flora Stephanie , Carol Johnson B. , Gleason Arthur , Yarbrough Mark , Feinholz Michael , Houlihan Terrance
Published: September 2021

A 3-D instrument self-shading correction has been developed for the MOBY upwelling radiance measurements. This correction was tested using the 23 year time series of MOBY measurements, at the Lanai, Hawaii site. The correction is small (less than 2%) except when the sun and collectors are aligned within 20° azimuth on opposite sides of the main MOBY structure. Estimates of the correction uncertainty were made with a Monte-Carlo method and the variation of the model input parameters at this site. The correction uncertainty was generally less than 1%, but increased to 30% of the correction in the strongest shadow region.
Virtual transparency in ϕ-OTDR using second order Raman amplification and pump modulation
Authors: Nuño Javier , Martin-Lopez Sonia , Ania-Castañón Juan D. , Gonzalez-Herraez Miguel , Martins Hugo F.
Published: September 2021

In distributed optical fibre sensors, distributed amplification schemes have been investigated in order to increase the measurement range while avoiding the limitation imposed by the fibre attenuation and the nonlinear effects. Recently, the use of Raman amplification with an engineered intensity modulation has been demonstrated as an efficient way to produce a virtually lossless trace employing a single-end configuration. In this paper, we propose the combination of this technique with a simultaneous second order Raman pumping scheme for increasing the measurement range. The optimal modulation profile has been numerically analyzed and we experimentally demonstrate a sensor able to detect perturbations along 70 km of fibre, with a minimal SNR penalty along the total length. Thanks to this new approach, the sensitivity in the worst point is considerably improved, and the ASD noise floor is also reduced. The measurement range is extended approximately 15 km compared with the equivalent first order pumping case.
Continuous-wave long-distributed-cavity laser using cat-eye retroreflectors
Authors: Sheng Quan , Wang Meng , Ma Hanchao , Qi Yue , Liu Junjie , Xu Degang , Shi Wei , Yao Jianquan
Published: September 2021

Here we demonstrate an efficient, long-distributed-cavity laser which uses a cat-eye retroreflector configuration to facilitate cavity alignment. The cavity parameters were optimized to meet the small stability region of the cavity, given the long working distance. We also found that intracavity spherical aberration seriously impacts laser efficiency, and an aspheric lens is used to correct the aberration when long working distance and receivers with compact dimensions are desired. The end-pumped Nd:YVO4 laser delivered 5.91 W continuous-wave output power at a long working distance of 5 m, under 16.6 W incident diode pump. Significantly, the fluctuation in output power over the whole working distance range of 1–5 m was less than 10%, this being achieved without any other cavity parameters being adjusted.
Multi-channel filter for UDWDM system designed based on stacking of Fabry-Perot etalons
Authors: Bhagavan Netheti V. S. , Msandeep Kumar M. , Krishna Podagatlapalli G.
Published: September 2021

The simulated multichannel comb filter [MCF] built on the stacking of the Fabry - Perot etalons that work in the telecommunication region between near-infra-red wavelengths (1538 −1558 nm) and (2333 - 2373 nm) has been demonstrated. Present work illustrates the dependence of defect modes produced on the stacked Fabry - Perot etalons. The FWHM of each channel is ∼0.043 picometers for the stacking of 20000 etalons. The spacing between the channels was ∼0.79 picometers and nearly uniform throughout the telecommunication band. The simulated MCF consists of ∼78 uniform channels within a range of ∼0.1 nm centered at 1550 nm.
The critical balance between dopamine D2 receptor and RGS for the sensitive detection of a transient decay in dopamine signal
Authors: Urakubo Hidetoshi , Yagishita Sho , Kasai Haruo , Kubota Yoshiyuki , Ishii Shin
Published: September 2021

In behavioral learning, reward-related events are encoded into phasic dopamine (DA) signals in the brain. In particular, unexpected reward omission leads to a phasic decrease in DA (DA dip) in the striatum, which triggers long-term potentiation (LTP) in DA D2 receptor (D2R)-expressing spiny-projection neurons (D2 SPNs). While this LTP is required for reward discrimination, it is unclear how such a short DA-dip signal (0.5–2 s) is transferred through intracellular signaling to the coincidence detector, adenylate cyclase (AC). In the present study, we built a computational model of D2 signaling to determine conditions for the DA-dip detection. The DA dip can be detected only if the basal DA signal sufficiently inhibits AC, and the DA-dip signal sufficiently disinhibits AC. We found that those two requirements were simultaneously satisfied only if two key molecules, D2R and regulators of G protein signaling (RGS) were balanced within a certain range; this balance has indeed been observed in experimental studies. We also found that high level of RGS was required for the detection of a 0.5-s short DA dip, and the analytical solutions for these requirements confirmed their universality. The imbalance between D2R and RGS is associated with schizophrenia and DYT1 dystonia, both of which are accompanied by abnormal striatal LTP. Our simulations suggest that D2 SPNs in patients with schizophrenia and DYT1 dystonia cannot detect short DA dips. We finally discussed that such psychiatric and movement disorders can be understood in terms of the imbalance between D2R and RGS.
Severe acute respiratory syndrome coronavirus (SARS-CoV-2) is not detected in the vagina: A prospective study
Authors: Takmaz Ozguc , Kaya Eren , Erdi Burak , Unsal Gozde , Sharifli Pari , Agaoglu Nihat Bugra , Ozbasli Esra , Gencer Serap , Gungor Mete
Published: September 2021

Objective

To determine whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is present in the vagina of women diagnosed with coronavirus disease-19 (COVID-19) pneumonia.

Study design

The study was conducted prospectively in a university affiliated hospital. Forty-one women of reproductive age whose nasopharyngeal PCR test were positive for SARS-CoV-2 and clinically diagnosed with pneumonia were included in the study. Vaginal swabs were obtained for SARS-CoV-2 PCR tests when the patients were admitted to the inpatient service before pneumonia treatment was initiated.

Results

Vaginal swab samples of 38 patients were analysed with SARS-CoV-2 PCR tests. None of the vaginal swabs were positive for SARS-CoV-2.

Conclusions

SARS-CoV-2 does not infect the vagina of women diagnosed with SARS-CoV-2 pneumonia.
An expanding HIV epidemic among older adults in Ukraine: Implications for patient-centered care
Authors: Rozanova Julia , Zeziulin Oleksandr , Rich Katherine M. , Altice Frederick L. , Kiriazova Tetiana , Zaviryukha Irina , Sosidko Tetiana , Gulati Komal , Carroll Constance , Shenoi Sheela V.
Published: September 2021

Introduction

The Eastern Europe and Central Asian (EECA) region has the highest increase in HIV incidence and mortality globally, with suboptimal HIV treatment and prevention. All EECA countries (except Russia) are low and middle-income (LMIC). While LMIC are home to 80% of all older people living with HIV (OPWH), defined as ≥50 years, extant literature observed that newly diagnosed OPWH represent the lowest proportion in EECA relative to all other global regions. We examined HIV diagnoses in OPWH in Ukraine, a country emblematic of the EECA region.

Methods

We analysed incident HIV diagnoses from 2015–2018 and mortality trends from 2016–2018 for three age groups: 1) 15–24 years; 2) 25–49 years; and 3) ≥50 years. AIDS was defined as CD4<200cells/mL. Mortality was defined as deaths per 1000 patients newly diagnosed with HIV within the same calendar year. Mortality rates were calculated for 2016, 2017, and 2018, compared to age-matched general population rates, and all-cause standardized mortality ratios (SMRs) were calculated.

Results

From 2015–2018, the proportion of OPWH annually diagnosed with HIV increased from 11.2% to 14.9% (p<0.01). At the time of diagnosis, OPWH were also significantly (p<0.01) more likely to have AIDS (43.8%) than those aged 25–49 years (29.5%) and 15–24 years (13.3%). Newly diagnosed OPWH had the same-year mortality ranging from 3 to 8 times higher than age-matched groups in the Ukrainian general population.

Conclusions

These findings suggest a reassessment of HIV testing, prevention and treatment strategies in Ukraine is needed to bring OPWH into focus. OPWH are more likely to present with late-stage HIV and have higher mortality rates. Re-designing testing practices is especially crucial since OPWH are absent from targeted testing programs and are increasingly diagnosed as they present with AIDS-defining symptoms. New strategies for linkage and treatment programs should reflect the distinct needs of this target population.
District level correlates of COVID-19 pandemic in India during March-October 2020
Authors: Tamrakar Vandana , Srivastava Ankita , Saikia Nandita , Parmar Mukesh C. , Shukla Sudheer Kumar , Shabnam Shewli , Boro Bandita , Saha Apala , Debbarma Benjamin
Published: September 2021

Background

COVID-19 is affecting the entire population of India. Understanding district level correlates of the COVID-19’s infection ratio (IR) is essential for formulating policies and interventions.

Objective

The present study aims to investigate the district level variation in COVID-19 during March-October 2020. The present study also examines the association between India’s socioeconomic and demographic characteristics and the COVID-19 infection ratio at the district level.

Data and methods

We used publicly available crowdsourced district-level data on COVID-19 from March 14, 2020, to October 31, 2020. We identified hotspot and cold spot districts for COVID-19 cases and infection ratio. We have also carried out two sets of regression analysis to highlight the district level demographic, socioeconomic, household infrastructure facilities, and health-related correlates of the COVID-19 infection ratio.

Results

The results showed on October 31, 2020, the IR in India was 42.85 per hundred thousand population, with the highest in Kerala (259.63) and the lowest in Bihar (6.58). About 80 percent infected cases and 61 percent deaths were observed in nine states (Delhi, Gujarat, West Bengal, Uttar Pradesh, Andhra Pradesh, Maharashtra, Karnataka, Tamil Nadu, and Telangana). Moran’s- I showed a positive yet poor spatial clustering in the COVID-19 IR over neighboring districts. Our regression analysis demonstrated that percent of 15–59 aged population, district population density, percent of the urban population, district-level testing ratio, and percent of stunted children were significantly and positively associated with the COVID-19 infection ratio. We also found that, with an increasing percentage of literacy, there is a lower infection ratio in Indian districts.

Conclusion

The COVID-19 infection ratio was found to be more rampant in districts with a higher working-age population, higher population density, a higher urban population, a higher testing ratio, and a higher level of stunted children. The study findings provide crucial information for policy discourse, emphasizing the vulnerability of the highly urbanized and densely populated areas.
Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk
Authors: Wijenayake Sanoji , Eisha Shafinaz , Tawhidi Zoya , Pitino Michael A. , Steele Michael A. , Fleming Alison S. , McGowan Patrick O.
Published: September 2021

Milk is a highly complex, heterogeneous biological fluid that contains non-nutritive, bioactive extracellular vesicles called exosomes. Characterization of milk-derived exosomes (MDEs) is challenging due to the lack of standardized methods that are currently being used for milk pre-processing, storage, and exosome isolation. In this study, we tested: 1) three pre-processing methods to remove cream, fat, cellular debris, and casein proteins from bovine milk to determine whether pre-processing of whole milk prior to long-term storage improves MDE isolations, 2) the suitability of two standard exosome isolation methods for MDE fractionation, and 3) four extraction protocols for obtaining high quality RNA from bovine and human MDEs. MDEs were characterized via Transmission Electron Microscopy (TEM), Nanoparticle Tracking Analysis (NTA), and western immunoblotting for CD9, CD63, and Calnexin protein markers. We also present an optimized method of TEM sample preparation for MDEs. Our results indicate that: 1) Removal of cream and fat globules from unpasteurized bovine milk, prior to long-term storage, improves the MDE yield but not purity, 2) Differential ultracentrifugation (DUC) combined with serial filtration is better suited for bovine MDE isolation compared to ExoQuick (EQ) combined with serial filtration, however both methods were comparable for human milk, and 3) TRIzol LS is better suited for RNA extraction from bovine MDEs isolated by EQ and DUC methods. 4) TRIzol LS, TRIzol+RNA Clean and Concentrator, and TRIzol LS+RNA Clean and Concentrator methods can be used for RNA extractions from human MDEs isolated by EQ, yet the TRIzol LS method is better suited for human MDEs isolated by DUC. The QIAzol + miRNeasy Mini Kit produced the lowest RNA yield for bovine and human MDEs.
Geographical variation in demand, utilization, and outcomes of hospital services for COVID-19 in Brazil: A descriptive serial cross-sectional study
Authors: Pereira Claudia Cristina de Aguiar , Martins Mônica , Lima Sheyla Maria Lemos , de Andrade Carla Lourenço Tavares , Soares Fernando Ramalho Gameleira , Portela Margareth Crisóstomo
Published: September 2021

Objective

To analyze the geographical variation in the provision of health services, namely in demand, patterns of utilization, and effectiveness in the Brazilian Health Regions in four different periods of the COVID-19 pandemic, from February 2020 to March 2021.

Methods

Descriptive serial cross-sectional study based on secondary data on COVID-19 hospitalizations from SIVEP-Gripe, a public and open-access database of Severe Acute Respiratory Illness records collected by the Brazilian Ministry of Health, and COVID-19 case notification data from Brasil.io, a repository of public data. Fifty-six epidemiological weeks were split into four periods. The following variables were considered for each Brazilian Health Region, per period: number of hospitalizations, hospitalizations per 100,000 inhabitants, hospitalizations per 100 new cases notified in the Health Region, percentage of hospitalizations with ICU use, percentages of hospitalizations with invasive and non-invasive ventilatory support, percentage of hospitalizations resulting in death and percentage of hospitalizations with ICU use resulting in death. Descriptive statistics of the variables were obtained across all 450 Health Regions in Brazil over the four defined pandemic periods. Maps were generated to capture the spatiotemporal variation and trends during the first year of the COVID-19 pandemic in Brazil.

Results

There was great variation in how COVID-19 hospitalizations grew and spread among Health Regions, with higher numbers between June and August 2020, and, especially, from mid-December 2020 to March 2021. The variation pattern in the proportion of ICU use in the hospitalizations across the Health Regions was broad, with no intensive care provision in large areas in the North, Northeast, and Midwest. The proportions of hospitalizations and hospitalizations with ICU use resulting in deaths were remarkably high, reaching medians of 34.0% and 62.0% across Health Regions, respectively.

Conclusion

The Heath Regions in Brazil are highly diverse, showing broad disparities in the capacity to respond to the demands imposed by COVID-19, services provided, use and outcomes.
Gazing through time and beyond the health sector: Insights from a system dynamics model of cardiovascular disease in Australia
Authors: Peng Cindy Q. , Lawson Kenny D. , Heffernan Mark , McDonnell Geoff , Liew Danny , Lybrand Sean , Pearson Sallie-Anne , Cutler Henry , Kritharides Leonard , Trieu Kathy , Huynh Quan , Usherwood Tim , Occhipinti Jo-An
Published: September 2021

Objective

To construct a whole-of-system model to inform strategies that reduce the burden of cardiovascular disease (CVD) in Australia.

Methods

A system dynamics model was developed with a multidisciplinary modelling consortium. The model population comprised Australians aged 40 years and over, and the scope encompassed acute and chronic CVD as well as primary and secondary prevention. Health outcomes were CVD-related deaths and hospitalisations, and economic outcomes were the net benefit from both the healthcare system and societal perspectives. The eight strategies broadly included creating social and physical environments supportive of a healthy lifestyle, increasing the use of preventive treatments, and improving systems response to acute CVD events. The effects of strategies were estimated as relative differences to the business-as-usual between 2019–2039. Probabilistic sensitivity analysis produced uncertainty intervals of interquartile ranges (IQR).

Findings

The greatest reduction in CVD-related deaths was seen in strategies that improve systems response to acute CVD events (8.9%, IQR: 7.7–10.2%), yet they resulted in an increase in CVD-related hospitalisations due to future recurrent admissions (1.6%, IQR: 0.1–2.3%). This flow-on effect highlighted the importance of addressing underlying CVD risks. On the other hand, strategies targeting the broad environment that supports a healthy lifestyle were effective in reducing both hospitalisations (7.1%; IQR: 5.0–9.5%) and deaths (8.1% reduction; IQR: 7.1–8.9%). They also produced an economic net benefit of AU$43.3 billion (IQR: 37.7–48.7) using a societal perspective, largely driven by productivity gains. Overall, strategic planning to reduce the burden of CVD should consider the varying effects of strategies over time and beyond the health sector.
Prevalence of HER2 overexpression and amplification in cervical cancer: A systematic review and meta-analysis
Authors: Itkin Boris , Garcia Agustin , Straminsky Samanta , Adelchanow Eduardo Daniel , Pereyra Matias , Haab Gabriela Acosta , Bardach Ariel
Published: September 2021

The reported rates of HER2 positivity in cervical cancer (CC) range from 0% to 87%. The importance of HER2 as an actionable target in CC would depend on HER2 positivity prevalence. Our aim was to provide precise estimates of HER2 overexpression and amplification in CC, globally and by relevant subgroups. We conducted a PRISMA compliant meta-analytic systematic review. We searched Medline, EMBASE, Cochrane database, and grey literature for articles reporting the proportion of HER2 positivity in CC. Studies assessing HER2 status by immunohistochemistry or in situ hybridization in invasive disease were eligible. We performed descriptive analyses of all 65 included studies. Out of these, we selected 26 studies that used standardized American Society of Clinical Oncology / College of American Pathologists (ASCO/CAP) Guidelines compliant methodology. We conducted several meta-analyses of proportions to estimate the pooled prevalence of HER2 positivity and subgroup analyses using geographic region, histology, tumor stage, primary antibody brand, study size, and publication year as moderators. The estimated pooled prevalence of HER2 overexpression was 5.7% (CI 95%: 1.5% to 11.7%) I2= 87% in ASCO/CAP compliant studies and 27.0%, (CI 95%: 19.9% to 34.8%) I2= 96% in ASCO/CAP non-compliant ones, p < 0.001. The estimated pooled prevalence of HER2 amplification was 1.2% (CI 95%: 0.0% to 5.8%) I2= 0% and 24.9% (CI 95%: 12.6% to 39.6%) I2= 86%, respectively, p = 0.004. No other factor was significantly associated with HER2 positivity rates. Our results suggest that a small, but still meaningful proportion of CC is expected to be HER2-positive. High heterogeneity was the main limitation of the study. Variations in previously reported HER2 positivity rates are mainly related to methodological issues.
Strangely mined bitcoins: Empirical analysis of anomalies in the bitcoin blockchain transaction network
Authors: Óskarsdóttir María , Mallett Jacky
Published: September 2021

The blockchain technology introduced by bitcoin, with its decentralised peer-to-peer network and cryptographic protocols, provides a public and accessible database of bitcoin transactions that have attracted interest from both economics and network science as an example of a complex evolving monetary network. Despite the known cryptographic guarantees present in the blockchain, there exists significant evidence of inconsistencies and suspicious behavior in the chain. In this paper, we examine the prevalence and evolution of two types of anomalies occurring in coinbase transactions in blockchain mining, which we reported on in earlier research. We further develop our techniques for investigating the impact of these anomalies on the blockchain transaction network, by building networks induced by anomalous coinbase transactions at regular intervals and calculating a range of network measures, including degree correlation and assortativity, as well as inequality in terms of wealth and anomaly ratio using the Gini coefficient. We obtain time series of network measures calculated over the full transaction network and three sub-networks. Inspecting trends in these time series allows us to identify a period in time with particularly strange transaction behavior. We then perform a frequency analysis of this time period to reveal several blocks of highly anomalous transactions. Our technique represents a novel way of using network science to detect and investigate cryptographic anomalies.
The effects of induced emotions on environmental preferences and behavior: An experimental study
Authors: Ibanez Lisette , Roussel Sébastien
Published: September 2021

Communication policies employed by policymakers and non-governmental organizations (NGOs) often appeal to the emotions to persuade people to adopt virtuous behavior. The aim of this paper is to study the impact of induced emotions on pro-environmental behavior (PEB). We design a three-stage laboratory experiment. In the first stage, we determine the level of the subjects’ environmental awareness. In the second stage, subjects read scripts that place them in realistic hypothetical scenarios designed to induce specific emotions. We implement a 2 x 2 in-between design by varying both the valence and social dimension of the four emotional states induced: happiness, sadness, pride and shame. In the third stage, subjects play a modified dictator game in which the recipient is an environmental non-governmental organization (ENGO). We show that the emotional states of subjects can influence PEB. In particular, negative emotions significantly reduce the average individual amount of donations made to ENGOs. We also find that the precise impact of the emotional states is more complex and appears to be dependent on individuals’ characteristics and awareness for environmental issues. For instance, in positive emotional states, men donate significantly less than women. In addition, a high level of environmental awareness increases donations in subjects experiencing shame and decreases their likelihood to donate when feeling pride. Also, we observe behavioral consistency for negative emotions and rather compensatory behavior for positive emotions.
Dental health assessed using panoramic radiograph and adverse events in chronic kidney disease stage 4–5 patients transitioning to dialysis and transplantation–A prospective cohort study
Authors: Järvisalo Mikko J. , Jokihaka Viljami , Hakamäki Markus , Lankinen Roosa , Helin Heidi , Koivuviita Niina S. , Hellman Tapio , Metsärinne Kaj
Published: September 2021

Background and aims

Oral health could potentially be a modifiable risk factor for adverse outcomes in chronic kidney disease (CKD) patients transitioning from predialysis treatment to maintenance dialysis and transplantation. We aimed to study the association between an index of radiographically assessed oral health, Panoramic Tomographic Index (PTI), and cardiovascular and all-cause mortality, major adverse cardiovascular events (MACEs) and episodes of bacteremia and laboratory measurements during a three-year prospective follow-up in CKD stage 4–5 patients not on maintenance dialysis at baseline.

Methods

Altogether 190 CKD stage 4–5 patients without maintenance dialysis attended panoramic dental radiographs in the beginning of the study. The patients were followed up for three years or until death. MACEs and episodes of bacteremia were recorded during follow-up. Laboratory sampling for C-reactive protein and leukocytes was repeated tri-monthly.

Results

PTI was not associated with baseline laboratory parameters or C-reactive protein or leukocytes examined as repeated measures through the 3-year follow-up. During follow-up, 22 patients had at least one episode of bacteremia, but only 2 of the bacteremias were considered to be of oral origin. PTI was not associated with incident bacteremia during follow-up. Thirty-six patients died during follow-up including 17 patients due to cardiovascular causes. During follow-up 42 patients were observed with a MACE. PTI was independently associated with all-cause (HR 1.074 95% CI 1.029–1.122, p = 0.001) and cardiovascular (HR 1.105, 95% CI 1.057–1.157, p<0.0001) mortality, as well as, incident MACEs (HR 1.071 95% CI 1.031–1.113, p = 0.0004) in the multivariable Cox models adjusted for age and kidney transplantation or CKD treatment modality during follow-up.

Conclusions

Radiographically assessed dental health is independently associated with all-cause and cardiovascular mortality and MACEs but not with the incidence of bacteremia in CKD stage 4–5 patients transitioning to maintenance dialysis and renal transplantation during follow-up.
Variation in winter site fidelity within and among individuals influences movement behavior in a partially migratory ungulate
Authors: Fullman Timothy J. , Person Brian T. , Prichard Alexander K. , Parrett Lincoln S.
Published: September 2021

Many animals migrate to take advantage of temporal and spatial variability in resources. These benefits are offset with costs like increased energetic expenditure and travel through unfamiliar areas. Differences in the cost-benefit ratio for individuals may lead to partial migration with one portion of a population migrating while another does not. We investigated migration dynamics and winter site fidelity for a long-distance partial migrant, barren ground caribou (Rangifer tarandus granti) of the Teshekpuk Caribou Herd in northern Alaska. We used GPS telemetry for 76 female caribou over 164 annual movement trajectories to identify timing and location of migration and winter use, proportion of migrants, and fidelity to different herd wintering areas. We found within-individual variation in movement behavior and wintering area use by the Teshekpuk Caribou Herd, adding caribou to the growing list of ungulates that can exhibit migratory plasticity. Using a first passage time–net squared displacement approach, we classified 78.7% of annual movement paths as migration, 11.6% as residency, and 9.8% as another strategy. Timing and distance of migration varied by season and wintering area. Duration of migration was longer for fall migration than for spring, which may relate to the latter featuring more directed movement. Caribou utilized four wintering areas, with multiple areas used each year. This variation occurred not just among different individuals, but state sequence analyses indicated low fidelity of individuals to wintering areas among years. Variability in movement behavior can have fitness consequences. As caribou face the pressures of a rapidly warming Arctic and ongoing human development and activities, further research is needed to investigate what factors influence this diversity of behaviors in Alaska and across the circumpolar Arctic.
Pesticides use and health impacts on farmers in Thailand, Vietnam, and Lao PDR: Protocol for a survey of knowledge, behaviours and blood acetyl cholinesterase concentrations
Authors: Hughes David , Thongkum Wisit , Tudpor Kukiat , Turnbull Niruwan , Yukalang Nachalida , Sychareun Vanphanom , Van Vo Thang , Win Latt Latt , Watkins Alan , Jordan Sue
Published: September 2021

Pesticides use in Southeast Asia has increased steadily, driven by the growth of large-scale commercial farming, as well as a desire to maximise food production in rural subsistence economies. Given that use of chemical pesticides, such as organophosphates and carbamates, has known potential health impacts, there are concerns about the safety of agricultural workers, and a need for a better evidence base to underpin regulation and worker education. This study, undertaken in 9 districts in Lao PDR, Thailand and Vietnam, will interview agricultural workers to investigate how they use pesticides, their knowledge of risks and self-protective practices, and their self-reported illness symptoms. In each district researchers will recruit and interview 120 participants engaged in vegetable farming, who have recently used pesticides, making a total of 1080 subjects divided equally between the three study countries. Workers’ degree of pesticides exposure will be determined from acetyl cholinesterase concentrations in capillary blood samples collected using field test kits, and these data will be analysed together with the interview findings. Country findings will be compared and contrasted, and general patterns noted. Knowledge gained about risky behaviours, self-protective practices and degree of association with serious pesticides exposure will assist policy makers and inform health improvement programmes.
Herpes simplex virus 1 evades cellular antiviral response by inducing microRNA-24, which attenuates STING synthesis
Authors: Sharma Nikhil , Wang Chenyao , Kessler Patricia , Sen Ganes C.
Published: September 2021

STING is a nodal point for cellular innate immune response to microbial infections, autoimmunity and cancer; it triggers the synthesis of the antiviral proteins, type I interferons. Many DNA viruses, including Herpes Simplex Virus 1 (HSV1), trigger STING signaling causing inhibition of virus replication. Here, we report that HSV1 evades this antiviral immune response by inducing a cellular microRNA, miR-24, which binds to the 3’ untranslated region of STING mRNA and inhibits its translation. Expression of the gene encoding miR-24 is induced by the transcription factor AP1 and activated by MAP kinases in HSV1-infected cells. Introduction of exogenous miR-24 or prior activation of MAPKs, causes further enhancement of HSV1 replication in STING-expressing cells. Conversely, transfection of antimiR-24 inhibits virus replication in those cells. HSV1 infection of mice causes neuropathy and death; using two routes of infection, we demonstrated that intracranial injection of antimiR-24 alleviates both morbidity and mortality of the infected mice. Our studies reveal a new immune evasion strategy adopted by HSV1 through the regulation of STING and demonstrates that it can be exploited to enhance STING’s antiviral action.
IRF3-mediated pathogenicity in a murine model of human hepatitis A
Authors: Sun Lu , Li You , Misumi Ichiro , González-López Olga , Hensley Lucinda , Cullen John M. , McGivern David R. , Matsuda Mami , Suzuki Ryosuke , Sen Ganes C. , Hirai-Yuki Asuka , Whitmire Jason K. , Lemon Stanley M.
Published: September 2021

HAV-infected Ifnar1-/- mice recapitulate many of the cardinal features of hepatitis A in humans, including serum alanine aminotransferase (ALT) elevation, hepatocellular apoptosis, and liver inflammation. Previous studies implicate MAVS-IRF3 signaling in pathogenesis, but leave unresolved the role of IRF3-mediated transcription versus the non-transcriptional, pro-apoptotic activity of ubiquitylated IRF3. Here, we compare the intrahepatic transcriptomes of infected versus naïve Mavs-/- and Ifnar1-/- mice using high-throughput sequencing, and identify IRF3-mediated transcriptional responses associated with hepatocyte apoptosis and liver inflammation. Infection was transcriptionally silent in Mavs-/- mice, in which HAV replicates robustly within the liver without inducing inflammation or hepatocellular apoptosis. By contrast, infection resulted in the upregulation of hundreds of genes in Ifnar1-/- mice that develop acute hepatitis closely modeling human disease. Upregulated genes included pattern recognition receptors, interferons, chemokines, cytokines and other interferon-stimulated genes. Compared with Ifnar1-/- mice, HAV-induced inflammation was markedly attenuated and there were few apoptotic hepatocytes in livers of infected Irf3S1/S1Ifnar1-/- mice in which IRF3 is transcriptionally-inactive due to alanine substitutions at Ser-388 and Ser-390. Although transcriptome profiling revealed remarkably similar sets of genes induced in Irf3S1/S1Ifnar1-/- and Ifnar1-/- mice, a subset of genes was differentially expressed in relation to the severity of the liver injury. Prominent among these were both type 1 and type III interferons and interferon-responsive genes associated previously with apoptosis, including multiple members of the ISG12 and 2’-5’ oligoadenylate synthetase families. Ifnl3 and Ifnl2 transcript abundance correlated strongly with disease severity, but mice with dual type 1 and type III interferon receptor deficiency remained fully susceptible to liver injury. Collectively, our data show that IRF3-mediated transcription is required for HAV-induced liver injury in mice and identify key IRF3-responsive genes associated with pathogenicity, providing a clear distinction from the transcription-independent role of IRF3 in liver injury following binge exposure to alcohol.
El exilio infantil de la Guerra Civil española en la URSS y México. Ayuda internacional, experiencias de vida y legados
Authors: Garrido Caballero Magdalena
Published: September 2021

El estudio propone un recorrido histórico por el exilio de los «niños de la guerra» que recalaron en México y la URSS durante la Guerra Civil española entre 1937 y 1938 y sus experiencias, a través de fuentes diversas, testimonios, memorias, bibliografía especializada, recabadas a lo largo de distintos proyectos de investigación, y se adentra en la narrativa audiovisual para abordar las percepciones de los exiliados, y también en la prensa para recoger su impacto en las sociedades de acogida, aspecto este último que ha de ser estudiado en profundidad para ahondar en los legados contemporáneos.
Synthesis, Characterization and Antimicrobial Evaluation of Novel Dimethyl
Triazene Incorporated Phenylamino Pyrimidine Derivatives
Authors: Jadvani Vidhya V. , Naliapara Yogesh T.
Published: September 2021

A series of novel (E)-4-(4-(3,3-dimethyltriaz-1-en-1-yl)phenyl)-N-phenylpyrimidin-2-amine derivatives
have been synthesized from the condensation of (E)-3-(dimethylamino)-1-(4-(E)-3,3-dimethyltriaz-
1-en-1-yl)phenyl)prop-2-en-1-one with several guanidinium hydro-chloride. The newly synthesized
compounds were examined for in vitro antimicrobial activity against some antibacterial and fungal
strains. The synthesized compounds were characterized by 1H NMR, 13C NMR, IR, mass and elemental
analyses.
Comparison of Two Different Treatment Methods to Treat the Temporomandibular Joint Disorders (Myofascial Pain) in a Short Period of Time - A Retrospective Study
Authors: Albageah Hamad Nasser , Alwakeel Abdulaziz Abdulhakim
Published: September 2021

BACKGROUND Temporomandibular joint(TMJ)is the third most common site of pain in the orofacial area, while the masseter muscle was the primary painful masticatory muscle. The temporal and frontal region were pain areas indicated by temporomandibular joint disorder (TMDs) patients. The purpose of this study was to compare two different treatment modalities, physical therapy and occlusal appliance to treat myofascial pain. METHODS This retrospective study comprises of all orofacial pain patients attending orofacial pain clinics of Dental University Hospital, King Saud University in Riyadh, Saudi Arabia. Patients were categorized into two groups, the first group: patients treated by the occlusal appliance (hard type). The second group: patients treated by physiotherapy home exercises, including posture position modification. Physical therapy included muscle stretching and isometric tension against resistance exercises and guided jaw movements. Methods of clinical examination was based on the research diagnostic criteria for temporomandibular disorders (RDC/TMD) criteria. The data of pain level was collected based on the visual analog scale (VAS). RESULTS 16.1 % of patients were male, and 83.9 % of the patients were female. With the mean age being 31.1 years old. 92.9 % were Saudi patients and 7.1 % were non-Saudi. 50 % of the patients were using an occlusal appliance, and 50 % went for physiotherapy. The independent t-test showed a highly significant difference between different management methods with a P – value of 0.038 and a mean difference of 0.32143. 80.5 % of the patients reported masseter muscle pain as one of their main complaints. CONCLUSIONS A significant difference was observed between physiotherapy and occlusal appliances with education in treating patients with myofascial pain. Patients using the occlusal appliances showed a high percentage of pain reduction (85.7 %) compared to physiotherapy treatment (57.1 %) in a short period of time. Henceforth, patient’s education plays a significant role in pain reduction. KEY WORDS Temporomandibular Joint Disorder, Occlusal Appliance, Myofascial Pain, Physiotherapy