bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptomic response for revealing the molecular mechanism of oat flowering under different photoperiods
Authors: Zhang Man , Jiang Yuan , Dong Haixiao , Shan Xiaohui , Tian Juan , Sun Moke , Ma Feiyue , Ren Changzhong , Yuan Yaping
Published: October 2023

Proper flowering is essential for the reproduction of all kinds of plants. Oat is an important cereal and forage crop; however, its cultivation is limited because it is a long-day plant. The molecular mechanism by which oats respond to different photoperiods is still unclear. In this study, oat plants were treated under long-day and short-day photoperiods for 10 days, 15 days, 20 days, 25 days, 30 days, 40 days and 50 days, respectively. Under the long-day treatment, oats entered the reproductive stage, while oats remained vegetative under the short-day treatment. Forty-two samples were subjected to RNA-Seq to compare the gene expression patterns of oat under long- and short-day photoperiods. A total of 634-5,974 differentially expressed genes (DEGs) were identified for each time point, while the floral organ primordium differentiation stage showed the largest number of DEGs, and the spikelet differentiation stage showed the smallest number. Gene Ontology (GO) analysis showed that the plant hormone signaling transduction and hormone metabolism processes significantly changed in the photoperiod regulation of flowering time in oat. Moreover, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Mapman analysis revealed that the DEGs were mainly concentrated in the circadian rhythm, protein antenna pathways and sucrose metabolism process. Additionally, transcription factors (TFs) involved in various flowering pathways were explored. Combining all this information, we established a molecular model of oat flowering induced by a long-day photoperiod. Taken together, the long-day photoperiod has a large effect at both the morphological and transcriptomic levels, and these responses ultimately promote flowering in oat. Our findings expand the understanding of oat as a long-day plant, and the explored genes could be used in molecular breeding to help break its cultivation limitations in the future.
A randomized controlled trial to evaluate the efficacy of metacognitive training for older adults with depression (MCT-Silver) in Portugal: study protocol
Authors: Pinho Lara Guedes de , Silva Celso , Fonseca César , Morgado Bruno , Lopes Manuel , Moritz Steffen , Jelinek Lena , Schneider Brooke C.
Published: October 2023

Introduction

Depression is one of the most common psychological disorders in later life. Although psychological interventions are recommended by treatment guidelines, most older adults with depression remain untreated. The aim of this study is to evaluate the efficacy of the Portuguese version of Metacognitive Training for Depression in later life (MCT-Silver).

Methods

This is a study protocol of an observer-blind, parallel-group, randomized controlled trial to compare the efficacy of MCT-Silver with a treatment as usual (TAU) control group among older adults (age 65 years and older) with depressive symptoms according to the Montgomery-Asberg Depression Scale. Participants will be tested at three assessment time points (baseline, immediately following the intervention [8 weeks], and 3 months after the intervention). The primary outcome is change in self-rated depression symptoms assessed by the Beck Depression Inventory (BDI-II). Secondary outcomes include clinician-rated depression, self-esteem, dysfunctional beliefs, metacognitive beliefs, ruminations, attitudes toward aging and quality of life. A self-designed subjective appraisal rating scale consisting of 21-items will be used to assess participant acceptance of MCT-Silver.

Discussion

MCT-Silver is an innovative intervention, which aims to reduce dysfunctional thoughts as well as depression-related behaviors and coping strategies through the metacognitive perspective. Until now, the training has only been tested in Germany. It is expected that after 8 weeks of treatment and 3 months later, the experimental group will demonstrate significant reductions in depressive symptoms, metacognitive beliefs, dysfunctional attitudes and ruminative responses compared to the TAU group. Moreover, quality of life, self-esteem, and attitudes towards aging will be significantly improved in MCT-Silver compared to the TAU group.

Clinical trial registration

ClinicalTrials.gov, NCT05640492.
Smoking cessation policy and treatments derived from the protective motivation of smokers: a study on graphic health warning labels
Authors: Pang Qiwei , Wang Lu , Yao Jinge , Yuen Kum Fai , Su Miao , Fang Mingjie
Published: October 2023

Introduction

Smoking is a leading public health risk. Many countries are reducing the demand for tobacco through graphic health warning labels (GHWLs). This study aims to explore smokers’ perceptions of GHWLs and analyze the effect of GHWLs on their behavioral intentions to quit smoking.

Methods

A theoretical model is designed by synthesizing protection motivation theory, an extension of the extended parallel process model, and the theory of planned behavior. We collected a cross-sectional sample of 547 anonymous smokers through a stratified random sampling strategy. GHWLs published in 2011 by the US Food and Drug Administration were used in the survey to assess smokers’ responses to them, and then the hypotheses are validated through structural equation models.

Results

The results suggest that perceived severity, perceived vulnerability, response efficacy, and health anxiety have a significant impact on smokers’ protection motivation. Furthermore, smokers’ protection motivation directly impacts the behavioral intention to quit smoking and indirectly influences intention to quit through attitudes.

Discussion

These findings have practical implications for the implementation and improvement of GHWLs policies. Meanwhile, this study enriches the literature on public health protection measures (i.e., GHWLs) and smokers’ behavioral intention to quit smoking.
The phenomenological model of depression: from methodological challenges to clinical advancements
Authors: Frohn Oskar Otto , Martiny Kristian Moltke
Published: October 2023

In this article our overall aim is to illustrate how phenomenological psychopathology can advance the clinical work on depression. To do so, we start by unfolding the current phenomenological model of depression. We argue that this model faces a methodological challenge, which we define as ‘the challenge of patho-description’. Mental disorders, such as depression, influence how people are able to access and describe their own experiences. This becomes a challenge for phenomenological psychopathology since its methodology is based on people’s ability to describe their own experiences. To deal with this challenge, in the case of depression, we turn to the framework of phenomenological interview. We interview 12 participants (7 women, 5 men, age-range from 29 to 57 years) with moderate and severe depression. From the interview results, we show how phenomenological interview deals with the challenge of patho-description and how patho-description in depression conceals experiential nuances. We unfold these nuances and describe how people with depression pre-reflectively experience a variety of feelings, a type of agency, overly positive self-image, and relations in a hyper-social way. These descriptive nuances not only strengthen the phenomenological model of depression, but they also help advance the clinical work on depression. We firstly illustrate how the descriptive nuances can be added to current manuals and rating scales to advance diagnostic work. Secondly, we illustrate how phenomenological, ‘bottom-up’, and embodied approaches function at the pre-reflective level of experience, and that further effort at this level can help advance therapy for depression.
Remote crisis intervention and suicide risk management in COVID-19 frontline healthcare workers
Authors: Robles Rebeca , Infante Sara , Feria Miriam , Arango Iván , Tirado Elsa , Rodríguez-Delgado Andrés , Miranda Edgar , Fresán Ana , Becerra Claudia , Escamilla Raul , Madrigal de León Eduardo Angel
Published: October 2023

Introduction

Despite the propagation of virtual mental health services for vulnerable groups during COVID-19 pandemic, the implementation and evaluation of remote evidence-based practices (EBP) to manage them in low- and middle-income countries remains scarce. In the current study, we describe and evaluate the implementation process and clinical impact of brief, remote, manualized EBP for crisis intervention and suicide risk management among healthcare workers attending patients with COVID-19 (COVID-19-HCWs) in Mexico.

Methods

The implementation process comprised community engagement of volunteer mental health specialists, creation of new clinical teams with different disciplines and skills, intervention systematization through manuals and education through 4-h remote training as main strategies. Mexican COVID-19-HCWs who had used a free 24-h helpline rated their pre- and post-intervention emotional distress. Therapists recorded patients’ pre-intervention diagnosis, severity, and suicide risk, the techniques used in each case, and their post-treatment perception of COVID-19-HCWs’ improvement at the end of the intervention.

Results

All techniques included in the intervention manual were employed at least in one case (n = 51). At the beginning of the intervention, 65.9% of the COVID-19-HCWs were considered moderately ill or worse according to Clinical Global Impression-Severity (CGI-S) scores, whereas at the end, 79.4% of them were perceived as much or very much improved according to CGI-Improvement scores (CGI-I), and their emotional distress had been significantly reduced (p < 0.001).

Discussion

This prospective study provides evidence that implementation of remote EBP is feasible and useful to reduce emotional distress and suicide risk among COVID-19-HCWs from a middle-income country. However, this study was limited by lack of a control group, improvement ratings provided by therapists and non-anonymous satisfaction ratings.
ICEBERG study: an indirect adjusted comparison estimating the long-term benefit of esketamine nasal spray when compared with routine treatment of treatment resistant depression in general psychiatry
Authors: Oliveira-Maia Albino J. , Morrens Joachim , Rive Benoit , Godinov Yordan , Cabrieto Jedelyn , Perualila Nolen , Barbreau Sebastien , Mulhern-Haughey Siobhán
Published: October 2023

Background

Treatment resistant depression (TRD) affects 10–30% of patients with major depressive disorder. In 4-week trials, esketamine nasal spray (NS) was efficacious vs. placebo when both were initiated in addition to a new selective serotonin or serotonin norepinephrine reuptake inhibitor. However, comparison with an extended range of real-world treatments (RWT) is lacking.

Methods

ICEBERG was an adjusted indirect treatment comparison using propensity score-based inverse probability weighting, performed on 6-month response and remission data from patients receiving esketamine NS plus oral antidepressant from the SUSTAIN-2 (NCT02497287; clinicaltrials.gov) study, compared with patients receiving other RWT from the European Observational TRD Cohort (EOTC; NCT03373253; clinicaltrials.gov) study. SUSTAIN-2 was a long-term open-label study of esketamine NS, while the EOTC was conducted at a time when esketamine NS was not available as RWT. Threshold and sensitivity analyses were conducted to assess how robust the primary analyses were.

Results

Patients receiving esketamine NS had a higher probability of 6-month response (49.7% [95% confidence interval (CI) 45.6–53.9]) and remission (33.6% [95% CI 29.7–37.6]) vs. patients receiving RWT (26.4% [95% CI 21.5–31.4] and 18.2% [95% CI 13.9–22.5], respectively), according to rescaled average treatment effect among treated estimates. Resulting adjusted odds ratios (OR) and relative risk (RR) favoured esketamine NS over RWT for 6-month response (OR 2.756 [95% CI 2.034–3.733], p < 0.0001; RR 1.882 [95% CI 1.534–2.310], p < 0.0001) and remission (OR 2.276 [95% CI 1.621–3.196], p < 0.0001; RR 1.847 [95% CI 1.418–2.406], p < 0.0001). Threshold analyses suggested that differences between the two studies were robust, and results were consistent across extensive sensitivity analyses.

Conclusion

ICEBERG supports that, at 6 months, esketamine NS has a substantial and significant benefit over RWT for patients with TRD. While results may be affected by unobserved confounding factors, threshold analyses suggested these were unlikely to impact the study conclusions.

To view an animated summary of this publication, please click on the Supplementary video.
Guiding principles for the implementation of a standardized psychological autopsy to understand and prevent suicide: a stakeholder analysis
Authors: Balt Elias , Heesen Karlijn , Popma Arne , Gilissen Renske , Mérelle Saskia , Creemers Daan
Published: October 2023

Background

Around 700,000 people die by suicide each year. While the global number of suicides declined over the last decade, the rates remained unchanged in the Netherlands. With this study, we aimed to provide guiding principles for the implementation of a national standardized psychological autopsy to better understand and prevent suicide, by exploring stakeholder perceptions and needs, and barriers to implementation.

Methods

We interviewed 47 representative stakeholders from various fields (e.g., mental healthcare professionals, policy advisors, researchers). A semi structured interview design was used, based on the RE-AIM and Consolidated Framework for Implementation Research (CFIR) theoretical frameworks.

Results

Themes relating to stakeholder perceptions and needs for a standardized psychological autopsy included valorization, accountability, integrability and the needs of the bereaved. Stakeholders believed that participation in a psychological autopsy can help bereaved in their process of grief but noted that evidence to frame the psychological autopsy as postvention is insufficient. The primary focal point should accordingly be to better understand and prevent suicide. Several key limitations of the proposed psychological autopsy approach were detailed, both methodological and implementational.

Conclusion

The stakeholder analysis delineates guiding principles for implementation. Stakeholders believe that a standardized psychological autopsy has merit, provided that key considerations, including valorization and accountability, are integrated in its design. Routine evaluation should be ensured. The findings may guide policy makers and researchers in their endeavors to support a learning, community-based approach for suicide prevention based on a standardized psychological autopsy.
Case report: 10 years follow-up of psychosis due to Fahr’s disease complicated by a left temporal stroke
Authors: De Pieri Marco , Poglia Greta , Bartolomei Javier
Published: October 2023

Fahr’s disease (FD) is a rare disorder, characterized by basal ganglia calcification and presenting with movement disorders, speech impairment, cognitive deficits, and neuropsychiatric symptoms. Psychotic disorders related to FD are barely described in the literature, and knowledge is missing concerning pathophysiology, course, and management. Here, we report on the long-term follow-up of a patient who had three acute episodes of FD-psychosis characterized by bizarre delusions and behavioral disorganization, without hallucinations. Genetic and metabolic causes of FD were ruled out. In all three episodes, olanzapine monotherapy rapidly and completely resolved psychosis, without inducing metabolic syndrome and extrapyramidal symptoms. In addition to the acute decompensations, the patient presented a tame, introverted, industrious, and perfectionistic personality, which we could interpret as the “parkinsonian personality” described for many other basal ganglia disorders. Moreover, bizarre appearance, reduced affectivity, abulia, concrete speech, and stiff motricity in the context of a mild asymmetric extrapyramidal syndrome characterized the mental status. The cognitive profile was initially marked by executive difficulties and partial agnosia, with an IQ of 86. In the course of 10 years, the patient suffered from an ischemic stroke in the left superior temporal gyrus, which provoked a decline in memory and executive functions, without any impact on the psychiatric picture. Antiphospholipid antibody syndrome emerged as the underlying cause; thus, for the first time in the literature, an overlap of FD and antiphospholipid antibody syndrome is described here. This case report stresses once more the need for better integration of psychiatry and neurology and for the investigation of secondary causes of late-onset psychosis.
Finite Element–Boundary Element Acoustic Backscattering with Model Reduction of Surface Pressure Based on Coherent Clusters
Authors: Krysl Petr , Abawi Ahmad T.
Published: October 2023

Computing backscattering of harmonic acoustic waves from underwater elastic targets of arbitrary shape is a problem of considerable practical significance. The finite element method is commonly applied to the discretization of the target; on the other hand, the boundary element method naturally incorporates the radiation boundary condition at infinity. The coupled model tends to be expensive, primarily due to the need to manipulate large, dense, and complex matrices and to repeatedly solve systems of complex linear algebraic equations of significant size for each frequency of interest. In this article, we develop a model reduction transformation based on the notion of coherence applied to the surface pressures, which considerably reduces the size of the systems to be solved. We found that the proposed model reduction approach delivers acceptably accurate results at a fraction of the cost of the full model. A typical speedup of an order of magnitude was realized in our numerical experiments. Our approach enables backscattering computations with considerably larger models than have been feasible to date.
Fast Trajectory Generation with a Deep Neural Network for Hypersonic Entry Flight
Authors: Li Haochen , Chen Haibing , Tan Chengpeng , Jiang Zaiming , Xu Xinyi
Published: October 2023

Optimal entry flight of hypersonic vehicles requires achieving specific mission objectives under complex nonlinear flight dynamics constraints. The challenge lies in rapid generation of optimal or near-optimal flight trajectories with significant changes in the initial flight conditions during entry. Deep Neural Networks (DNNs) have shown the capability to capture the inherent nonlinear mapping between states and optimal actions in complex control problems. This paper focused on comprehensive investigation and evaluation of a DNN-based method for three-dimensional hypersonic entry flight trajectory generation. The network is designed using cross-validation to ensure its performance, enabling it to learn the mapping between flight states and optimal actions. Since the time-consuming training process is conducted offline, the trained neural network can generate a single optimal control command in about 0.5 milliseconds on a PC, facilitating onboard applications. With the advantages in mapping capability and calculating speed of DNNs, this method can rapidly generate control action commands based on real-time flight state information from the DNN model. Simulation results demonstrate that the proposed method maintains a high level of accuracy even in scenarios where the initial flight conditions (including altitude, velocity, and flight path angle) deviate from their nominal values, and it has certain generalization ability.
Effect of Calcium Cyanamide as an Alternative Nitrogen Source on Growth, Yield, and Nitrogen Use Efficiency of Short-Day Onion
Authors: Simelane Mzwakhile Petros Zakhe , Soundy Puffy , Maboko Martin Makgose
Published: October 2023

Effective nitrogen (N) management in agriculture is vital to optimize crop growth and yield while minimizing environmental impact. Conventional nitrogen (N) sources, such as urea, have limitations in promoting growth and reducing N leaching. A two-year field experiment was carried out to investigate the effects of calcium cyanamide (CaCN2) as a slow-release N source on short-day onion growth, yield, and N use efficiency (NUE). Six types of N sources were administered: (i) an initial application of 80 kg ha−1 N in the form of CaCN2 before planting; (ii) an initial application of 80 kg ha−1 N in the form of CaCN2 before planting, followed by a topdressing of 50 kg ha−1 N in the form of limestone ammonium nitrate (LAN); (iii) an initial application of 80 kg ha−1 N in the form of CaCN2 before planting, followed by a topdressing of 50 kg ha−1 N in the form of urea; (iv) an initial application of 80 kg ha−1 N in the form of LAN before planting, followed by a topdressing of 50 kg ha−1 N in the form of LAN; (v) an initial application of 80 kg ha−1 N in the form of urea before planting, followed by a topdressing of 50 kg ha−1 N in the form of urea; and (vi) control (0 kg ha−1 N). Preplant CaCN2 (80 kg ha−1 N) outperformed the standard fertilizers used in onion as an N source (urea and LAN) by improving growth and yield, and reducing N leaching. Preplant CaCN2 topdressed with either LAN or urea led to a significant increase in plant growth and total yield compared to using LAN or urea alone. The application of CaCN2, followed by topdressing with either LAN or urea, decreased onion bolting by 1.6% and 1.83%, respectively, compared to the control. The study suggests that applying LAN or urea as a topdressing to preplant CaCN2 enhances N utilization efficiency, leading to increased onion bulb yield and quality while reducing N leaching. This approach can help mitigate farm-level environmental pollution and provide valuable insights for improving onion production and sustainable agriculture practices in South Africa.
Environmental Consequences of Shelf Life Extension: Conventional versus Active Packaging for Fresh-Cut Salads
Authors: Villanova-Estors Raquel , Murcia-Velasco Diana Alexandra , Correa-Guimarães Adriana , López-Carballo Gracia , Hernández-Muñoz Pilar , Gavara Rafael , Navas-Gracia Luis Manuel
Published: October 2023

The use of active coatings in fresh food packaging is an innovative technique that optimizes the functional properties of films, resulting in a longer product shelf life and reduced food waste. But, which is more sustainable, active packaging (AP) or conventional packaging (CP) for the packaging of fresh-cut products? To answer this research question, this study analyzes the environmental performance of AP during its life cycle for packaging a minimally processed fresh salad mix compared with CP, in terms of its manufacture and use. The AP is a bag that includes a bioactive component, oregano essential oil (OEO), which is an inhibitor of microbial growth, incorporated into an ethylene vinyl alcohol copolymer (EVOH) coating on a conventional polypropylene (PP) film. To this end, a Life Cycle Assessment (LCA) was carried out based on ISO 14040 and 14044, using the ReCiPe methodology. The results showed that using active packaging has a beneficial affect, reducing the amount of produced food by 30% compared with conventional packaging over the same period. The reductions in the studied impact categories were greater than 50% in most of them, with a 62% reduction in global warming. The proposed sensitivity analysis showed the difference between the disposal or treatment of waste generated by the packaging production process and the packaged product, indicating that this step is of great importance for the environmental impacts and sustainability of this process. In 80% of the scenarios analyzed, the AP achieved better results than the CP in terms of damage categories.
Using Partial Least Squares and Regression to Interpret Temperature and Precipitation Effects on Maize and Soybean Genetic Variance Expression
Authors: Ashworth Amanda J. , Allen Fred L. , Saxton Arnold M.
Published: October 2023

Partial least squares (PLS) is a statistical technique that can evaluate the association of large numbers of external environmental variables with biological responses. PLS is a good method for analyzing the relative importance of variables and compressing the data for regression analyses. The objective of this study was to use PLS and regression analyses on soybean (Glycine max L.) and maize (Zea mays L.) variety trial results for five (soybean) or three (maize) maturity group (MG) tests, at five Tennessee locations spanning 14 years, in order to determine the environmental effects (weekly minimum and maximum air temperature and precipitation) on the expression of yield genetic variance (Vg). Overall, PLS excelled at identifying combinations of weather variables to develop models with high R2 values (41–59%) relative to the regression analysis (R2 = 34–44%), but they did not address the effects of specific variables as in regression analysis. In both maize and soybean, differences in genetic variance occurred among MG tests and locations. Overall, precipitation was the driving variable for maize Vg, indicating maize is more sensitive to rain events during the growing season than soybean, i.e., with each cm of precipitation, maize Vg increased by 11.38–23.78 (Mg ha−1)2. The results suggest that ensuring adequate water, particularly during weeks 3 and 6, is critical for maize Vg, regardless of the MG test and location. Genetically modified soybean cultivars responded similarly to conventional cultivars, suggesting no Vg response differences due to the glyphosate tolerance trait. These results have important implications for irrigation timing for the maximum expression of genetic differences in maize and soybean cultivars, particularly for management planning during future stochastic weather events.
Yield Performance of Intercropped Marantha arundinacea L. (Arrowroot) in Two Rubber Plantation Designs
Authors: Huang Jianxiong , Pan Jian , Wei Yuanxu , Dong Tianzhan , Zhang Xiaoyan , Tu Hanqi , Wang Xiuquan
Published: October 2023

Developing rubber agroforestry systems is crucial to ensure the sustainable development of natural rubber cultivation. This study focuses on the starch crop Maranta arundinacea (arrowroot) and assesses its productivity and influencing factors when intercropped in 6–7-year-old conventional single-row and double-row rubber plantations. We analyze various aspects, including light resources, root distribution, soil nutrients, arrowroot growth characteristics, and product quality. The results indicate that the daily average photosynthetically active radiation (PAR) in the double-row rubber agroforestry system intercropping area ranges from 896.4 to 940.2 μmol·m−2·s−1. Additionally, the rubber tree roots near the intercropping area are less dense (107.0 g cm−3). In contrast, the conventional single-row rubber agroforestry system has a significantly lower daily average PAR of only 145.7 μmol·m−2·s−1, and the nearby rubber tree roots are more abundant (616.2 g cm−3). Although soil nutrient levels were slightly lower in the intercropping area on the double-row treatment compared to the single-row treatment, there was no statistical difference (p < 0.05). Arrowroot’s photosynthetic capacity in the double-row rubber agroforestry system intercropping area is significantly greater than in the single-row rubber agroforestry system intercropping area. The yield per unit area in the former (23.46–27.47 t·ha−1) is also significantly higher than in the latter (2.87–4.75 t·ha−1, p < 0.05), with higher starch content. Therefore, arrowroot exhibits higher productivity when intercropped in double-row rubber agroforestry systems, making it suitable for establishing a “rubber–arrowroot” agroforestry model to enhance the yield per unit area of rubber plantations.
An Epidemiological Assessment of Cryptosporidium and Giardia spp. Infection in Pet Animals from Taiwan
Authors: Hsu Chia-Hui , Liang Chi , Chi Shi-Chien , Lee Kuan-Ju , Chou Chung-Hsi , Lin Chen-Si , Yang Wen-Yuan
Published: October 2023

Cryptosporidium spp. and Giardia duodenalis are enteric protozoan pathogens in humans. and animals. Companion animals infected with zoonotic species/assemblages are a matter of major public concern around the world. The objectives of the present study were to determine the prevalences of Cryptosporidium spp. and G. duodenalis infections and their co-infection statuses in dogs and cats living in Taiwan and to identify the species and assemblages. Fecal samples were collected from local animal shelters (n = 285) and a veterinary hospital (n = 108). Nested polymerase chain reaction (PCR) was performed using the SSU-rRNA, β-giardin, and glutamate dehydrogenase genes for Cryptosporidium spp. and G. duodenalis, respectively. Results showed that the overall prevalences of Cryptosporidium and G. duodenalis were 7.38% (29/393) and 10.69% (42/393). In addition, co-infection was detected in 1.02% (4/393) of all samples. Sample source, clinical sign, and breed may be risk factors that influence the infection rate. In Cryptosporidium-positive samples, C. canis and C. felis were detected most frequently. Although the canine-specific assemblages C and D (37/42) were dominant, the zoonotic human-specific assemblage A (1/42) was also found in Giardia-positive samples. Phylogenetic analysis revealed that most positive samples belonged to host-specific subtypes/assemblages, while some Cryptosporidium or Giardia-positive samples could be zoonotic. The findings suggested that pet animals could be a cause of zoonotic transmission, causing human cryptosporidiosis and giardiasis in Taiwan.
Rhodotorula mucilaginosa ZTHY2 Attenuates Cyclophosphamide-Induced Immunosuppression in Mice
Authors: Kang Kai , Deng Xinyi , Xie Weitian , Chen Jinjun , Lin Hongying , Chen Zhibao
Published: October 2023

Rhodotorula mucilaginosa (R. mucilaginosa) can enhance the immune and antioxidant function of the body. However, whether R. mucilaginosa has an immunoregulatory effect on cyclophosphamide (CTX)-induced immunosuppressed animals remains to be clarified. In this study, the R. mucilaginosa ZTHY2 that we isolated from the coastal waters of the South China Sea previously was prepared in order to investigate its immunoprotective effect on CTX-induced immunosuppression in mice, and the effects were compared to those of Lactobacillus acidophilus (LA) (a well-known probiotic). Seventy-two male SPF mice were divided into six groups: The C group (control); IM group (immunosuppressive model group) (+CTX); Rl, Rm, and Rh groups (+CTX+low, medium, and high concentration of R. mucilaginosa, respectively); and PC (positive control) group (+CTX+LA). After a 28-day feeding trial, blood samples were taken for biochemical and serum immunological analysis, and the thymus and spleen were collected to analyze the organ index, lymphocyte proliferation and differentiation, and antioxidant capacity. The findings showed that R. mucilaginosa ZTHY2 improved the spleen and thymus indices, effectively attenuated immune organ atrophy caused by CTX, and enhanced the proliferation of T and B lymphocytes induced by ConA and LPS. R. mucilaginosa ZTHY2 promoted the secretion of cytokines and immunoglobulins and significantly increased the contents of IL-2, IL-4, IL-6, TNF-α, IFN-γ, IgA, IgG, IgM, CD4, CD8, CD19, and CD20 in serum. The proportion of CD4+, CD8+, CD19+, and CD20+ lymphocytes in spleen, thymus, and mesenteric lymph nodes were increased. In addition, R. mucilaginosa ZTHY2 reduced the reactive oxygen species (ROS) and malondialdehyde (MDA) levels and increased glutathione (GSH), total superoxide dismutase (SOD), and catalase (CAT) levels. Our results indicated that R. mucilaginosa ZTHY2 can significantly enhance the immune function of immunosuppressed mice, and improving antioxidant capacity thus attenuates CTX-induced immunosuppression and immune organ atrophy.
Evaluation of the Available Energy Value and Amino Acid Digestibility of Brown Rice Stored for 6 Years and Its Application in Pig Diets
Authors: He Beibei , Shi Jingjing , Liu Kuanbo , Cheng Junlin , Wang Weiwei , Wang Yongwei , Li Aike
Published: October 2023

Long-term storage may reduce the nutritional quality of brown rice, so the present study aimed to evaluate the nutritional values of long-term-stored nutrition in pig diets. In Exp. 1, 18 Landrace × Yorkshire (L × Y) barrows with an initial body weight (IBW) of 25.48 ± 3.21 kg were randomly assigned to three treatments, including a corn-based diet, one-year-stored brown rice (BR1) diet, and six-year-stored brown rice (BR6) diet, to determine the digestible energy (DE) and metabolizable energy (ME) values of stored brown rice. In Exp. 2, 24 barrows (L × Y; IBW: 22.16 ± 2.42 kg) fixed with ileal T-cannula were randomly allotted to four dietary treatments, including a corn diet, two stored brown rice diets, and a nitrogen-free diet, to evaluate the amino acid (AA) digestibility of the stored brown rice. In Exp. 3 and 4, 108 crossbred weaned piglets (L × Y; IBW: 9.16 ± 0.89 kg) and 90 crossbred growing pigs (L × Y; IBW: 48.28 ± 3.51 kg) were allotted to three treatment diets, including a control diet and two stored brown rice diets, respectively, to investigate the application of stored brown rice in weaned piglets and fully grown pig diets. The results indicated that there was no significant difference in the DE and ME values between corn and stored brown rice (p > 0.05), while the apparent ileal digestibility (AID) of arginine, histidine, asparagine + aspartic acid (Asx), and the standardized ileal digestibility (SID) of arginine and histidine were higher in the stored brown rice diet compared to the corn diet (p < 0.05). Compared to the corn, the stored brown rice showed no significant effects on growth performance, nutrient-apparent total tract digestibility (ATTD), and serum biochemical indices (p > 0.05) but showed decreased activity in the various digestive enzymes in the duodenum, jejunum, and ileum of the weaned piglets (p < 0.05). Also, the stored brown rice diet showed no significant effects on growth performance, carcass traits, meat quality, as well as the fatty acid profiles in the longissimus dorsi muscle of fully grown pigs compared with the corn diet (p > 0.05). In conclusion, the brown rice stored for 6 years under good conditions had no obvious changes in the available energy and nutrient values. Although it may reduce digestive enzyme activity in the small intestines of the piglets, the stored brown rice showed no obvious adverse effects on growth performance and meat quality and can be effectively used in pig diets.
Increased Cattle Feeding Precision from Automatic Feeding Systems: Considerations on Technology Spread and Farm Level Perceived Advantages in Italy
Authors: Romano Elio , Brambilla Massimo , Cutini Maurizio , Giovinazzo Simone , Lazzari Andrea , Calcante Aldo , Tangorra Francesco Maria , Rossi Paolo , Motta Ambra , Bisaglia Carlo , Bragaglio Andrea
Published: October 2023

Automation reduces the impact of farming on climate change and helps farmers adapt to its financial impact. Automatic feeding systems (AFSs) increase the ruminant’s feeding precision and ease operators’ workload. Such systems exist on a spectrum, requiring varied levels of operator support and installation complexity. A recent survey on farmers pointed out that those already running an AFS and those willing to buy one appreciate its ease of use, the resulting animal welfare, and the resulting overall benefits (increased production, farm profitability, and reduced feed waste). Whether technologically complex or not, studies have confirmed the benefits that farmers perceive to be underlying the remarkable reduction in the environmental impact of feeding operations (AFSs are electrically powered), the increase in animal welfare resulting from reduced conflicts around accessing the feed bunk, and the constant availability of fresh feed. However, their introduction should follow accurate and holistic structural and economic planning for existing and newly built facilities. The availability of public subsidies plays an essential role in pushing farmers to adopt the most modern digital technologies, whose benefits may even increase when farmers couple them with interconnected sensors to monitor animals’ physiological states.
Oxidative Stress Mediated Therapy in Patients with Rheumatoid Arthritis: A Systematic Review and Meta-Analysis
Authors: Djordjevic Katarina , Milojevic Samanovic Andjela , Veselinovic Mirjana , Zivkovic Vladimir , Mikhaylovsky Victor , Mikerova Maria , Reshetnikov Vladimir , Jakovljevic Vladimir , Nikolic Turnic Tamara
Published: October 2023

Objective: The aim of this meta-analysis is to explore all the available literature to obtain updated data about the potential use of antioxidants in the treatment of rheumatoid arthritis (RA) and its ability to reduce disease progression and cardiovascular risk. Methods: This systematic review and meta-analysis was performed strictly in accordance with the PRISMA guidelines. English and Chinese databases were searched with a retrieval time up to March 2023. These databases included the PubMed, Embase, Medline Complete, Web of Sciences and Cochrane Collaboration, Wanfang, China National Knowledge Infrastructure, and VIP databases. This literature search was formulated by the two researchers independently. The search strategy consists of reading, collecting the literature, and conducting the preliminary screening. After that, they provide the final selection of the literature according to the inclusion criteria and data extraction. Also, for all studies, the risk bias was assessed to evaluate the quality of the included references. The content of the risk assessment of bias included the following criteria: random allocation method, allocation plan hiding, blind method, completeness of result data, and selectivity of reporting of results, as well as other biases. The main outcomes were clinical efficiency of antioxidant therapy (C-reactive protein, DAS28 score, HAQ, Number of tender joints, etc.) and oxidative stress indicators (catalase, superoxide dismutase, or total antioxidant capacity). Results: We observed, in most of the studies, the small or moderate effects of antioxidant treatment. The mean effect size is 0.525, and that means that moderate effects were observed in 30 selected RCTs. Also, this effect is confirmed in the 1652 patients with RA with the mean confidence interval of 0.276 (lower limit) and 0.983 (upper limit). Cohen coefficient was calculated at 0.05. Conclusion: The existing evidence is that antioxidants can reduce systemic and local oxidative stress and can reduce damage as the main agent involved in autoimmune diseases such as rheumatoid arthritis.
Replacement of Vitamin E by an Extract from an Olive Oil By-Product, Rich in Hydroxytyrosol, in Broiler Diets: Effects on Growth Performance and Breast Meat Quality
Authors: Corrales Nereida L. , Sevillano Fernando , Escudero Rosa , Mateos Gonzalo G. , Menoyo David
Published: October 2023

The hypothesis of this experiment was that a liquid rich in hydroxytyrosol (HT) obtained from “alperujo”, an olive oil by-product, could replace part of the added vitamin E (VE) as an antioxidant in poultry diets. There were five diets that differed exclusively in the substitution of supplemental VE (0 to 40 mg/kg, with differences of 10 mg/kg) by HT (30 to 0 mg/kg, with differences of 7.5 mg/kg). The basal diet was based on corn and soybean meal and provided 10 mg VE/kg. From 0 to 39 d of age, the growth performance of the birds was not affected by diet. The birds were slaughtered at 39 d of age to evaluate the quality of the breast, and malonaldehyde concentration, pH, color, and drip loss were measured. In terms of meat lipid oxidation, the combination of 22.5 mg HT/kg and 10 mg of added VE/kg equalized to a diet supplemented with 40 mg VE/kg. Meat color improved in broilers fed 7.5 mg HT/kg and 30 mg VE/kg. It is concluded that once the nutritional requirements of the birds in VE are satisfied, the dietary supplementation with the olive oil by-product rich in HT can be used as a strategy to spare VE in broiler diets.
The Anti-Oxidative, Anti-Inflammatory, Anti-Apoptotic, and Anti-Necroptotic Role of Zinc in COVID-19 and Sepsis
Authors: Briassoulis George , Briassoulis Panagiotis , Ilia Stavroula , Miliaraki Marianna , Briassouli Efrossini
Published: October 2023

Zinc is a structural component of proteins, functions as a catalytic co-factor in DNA synthesis and transcription of hundreds of enzymes, and has a regulatory role in protein–DNA interactions of zinc-finger proteins. For many years, zinc has been acknowledged for its anti-oxidative and anti-inflammatory functions. Furthermore, zinc is a potent inhibitor of caspases-3, -7, and -8, modulating the caspase-controlled apoptosis and necroptosis. In recent years, the immunomodulatory role of zinc in sepsis and COVID-19 has been investigated. Both sepsis and COVID-19 are related to various regulated cell death (RCD) pathways, including apoptosis and necroptosis. Lack of zinc may have a negative effect on many immune functions, such as oxidative burst, cytokine production, chemotaxis, degranulation, phagocytosis, and RCD. While plasma zinc concentrations decline swiftly during both sepsis and COVID-19, this reduction is primarily attributed to a redistribution process associated with the inflammatory response. In this response, hepatic metallothionein production increases in reaction to cytokine release, which is linked to inflammation, and this protein effectively captures and stores zinc in the liver. Multiple regulatory mechanisms come into play, influencing the uptake of zinc, the binding of zinc to blood albumin and red blood cells, as well as the buffering and modulation of cytosolic zinc levels. Decreased zinc levels are associated with increasing severity of organ dysfunction, prolonged hospital stay and increased mortality in septic and COVID-19 patients. Results of recent studies focusing on these topics are summarized and discussed in this narrative review. Existing evidence currently does not support pharmacological zinc supplementation in patients with sepsis or COVID-19. Complementation and repletion should follow current guidelines for micronutrients in critically ill patients. Further research investigating the pharmacological mechanism of zinc in programmed cell death caused by invasive infections and its therapeutic potential in sepsis and COVID-19 could be worthwhile.
U2-Net: A Very-Deep Convolutional Neural Network for Detecting Distracted Drivers
Authors: Alsrehin Nawaf O. , Gupta Mohit , Alsmadi Izzat , Alrababah Saif Addeen
Published: October 2023

In recent years, the number of deaths and injuries resulting from traffic accidents has been increasing dramatically all over the world due to distracted drivers. Thus, a key element in developing intelligent vehicles and safe roads is monitoring driver behaviors. In this paper, we modify and extend the U-net convolutional neural network so that it provides deep layers to represent image features and yields more precise classification results. It is the basis of a very deep convolution neural network, called U2-net, to detect distracted drivers. The U2-net model has two paths (contracting and expanding) in addition to a fully-connected dense layer. The contracting path is used to extract the context around the objects to provide better object representation while the symmetric expanding path enables precise localization. The motivation behind this model is that it provides precise object features to provide a better object representation and classification. We used two public datasets: MI-AUC and State Farm, to evaluate the U2 model in detecting distracted driving. The accuracy of U2-net on MI-AUC and State Farm is 98.34 % and 99.64%, respectively. These evaluation results show higher accuracy than achieved by many other state-of-the-art methods.
Reasons for Removal of Miniplates Used in Fixation of Maxillofacial Bone Fractures: Systematic Review and Meta-Analysis
Authors: Jaber Mohamed , Abouseif Nadin , Ibrahim Noor , Hassan Mawada , El-Ameen Alaa Mohamed
Published: October 2023

Maxillofacial fractures are a prevalent occurrence, and the widespread use of miniplates to stabilize and secure these fractures during surgery is common. However, the post-surgical fate of these miniplates remains a subject of contention among surgeons. Some advocate for their removal, while others suggest leaving them in place. This study aims to perform an extensive meta-analysis of pertinent studies to ascertain the destiny of miniplates employed in maxillofacial surgery and discuss the consequences of either their extraction or retention. To execute this meta-analysis, a search was conducted across number of databases, including PubMed, Google Scholar, ResearchGate, Embase, and Scopus. Keywords such as “titanium miniplates”, “screws”, “maxillofacial surgery”, “complications”, and “removal” were utilized to identify pertinent studies. The search was constrained to studies published between 1989 and 2022. The analysis encompassed only studies reporting outcomes related to miniplates in maxillofacial surgery, including complication rates and removal frequencies. Eleven studies conformed to the inclusion criteria and were incorporated into the meta-analysis, encompassing a total of 2240 patients, ten of which were retrospectives and one prospective, with the largest study involving 434 patients. The overall complication rate was determined to be 13.3%. The most prevalent reasons for miniplate removal included patient preference, tooth extraction, and infections. The average time span for miniplate removal was 10.7 months, with the most prolonged reported duration being 24 years. These findings imply that immediate removal might not be imperative in the majority of instances. In conclusion, our meta-analysis underscores the necessity of removing miniplates in cases where hardware leads to a variety of complications and physical discomfort. Principal factors driving plate removal included patient requests, tooth extractions, and infections. Ordinarily, plate removal took place within the span of 6 to 12 months, with the mandible being the most frequent site for removal. These findings accentuate the significance of extended monitoring to assess the trajectory of miniplates in maxillofacial surgery and provide valuable insights for future clinical decisions. To validate these conclusions and gain deeper insights into the factors influencing plate removal in maxillofacial surgery, further research with larger participant cohorts and standardized outcome reporting is imperative.
An Experimental Study on the Effect of Magmatic Thermal Evolution on the Molecular Structure of Low-Rank Coal
Authors: Zhao Wei , Jiang Jingyu , Huang Yongzhen , Zhang Fang , He Wanxing
Published: October 2023

Low-rank coal accounts for over half of China’s proven coal reserves. The possibility of coal and gas outbursts in low-rank coal is higher, especially in the m·agmatic thermal evolution area. The complexity of coal’s molecular structure is one of the reasons for problems during the process of mining operations. Different analysis techniques, including XRD, FTIR and Raman spectroscopy, were used to obtain the molecular characteristics of magmatic thermal evolution coal samples and normal coal samples, so that a comparative study could be carried out to investigate the influence of the magmatic thermal evolution effect on the molecular structure of low-rank coal. The ranges of the aromatic interlayer spacing (d002), average stacking heights (Lc) and stacking layer number (Nave) of the thermally evolved coal samples are 3.41–3.51 Å, 22.76–27.02 Å, and 6.68–7.70, respectively. The ranges of the full width at half maximum ratio (FD1/FG) and the peak integral intensity ratio (ID1/IG) are 2.16–2.19 and 1.55–1.84, respectively. Compared with the normal coal samples, those affected by magmatic thermal evolution have smaller d002, ID1/IG, and FD1/FG values, but larger Lc values. The results indicate that the thermally evolved coal samples have more ordered structures and more developed microcrystalline structure sizes than normal coal samples.