Assessment of Cerebral Tumors and Metastases by Two-Photon Excitation Microscopy
Authors:   Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, Romania Carol Davila University Central Emergency Military Hospital, Bucharest, Romania Carol Davila University of Medicine and Pharmacy, Bucharest, Roman, Enache Adrian , Eftimie Lucian G. ,   Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, Romania Carol Davila University Central Emergency Military Hospital, Bucharest, Romania Department of Special Motricity and Medical Recovery, The National, Graur Ana M. ,   Carol Davila University Central Emergency Military Hospital, Bucharest, Romania, Glogojeanu Remus R. ,   Department of Special Motricity and Medical Recovery, The National University of Physical Education and Sports, Bucharest, Romania, Geambașu Adina ,   Department of Special Motricity and Medical Recovery, The National University of Physical Education and Sports, Bucharest, Romania, Voinea Oana C. ,   Carol Davila University of Medicine and Pharmacy, Bucharest, Romania, Costache Daniel O. ,   Carol Davila University Central Emergency Military Hospital, Bucharest, Romania Carol Davila University of Medicine and Pharmacy, Bucharest, Romania, Costache Raluca S. ,   Carol Davila University Central Emergency Military Hospital, Bucharest, Romania Carol Davila University of Medicine and Pharmacy, Bucharest, Romania, Sajin Maria ,   Carol Davila University of Medicine and Pharmacy, Bucharest, Romania, Stanciu George A. ,   Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, Romania
Published: August 2023

Objective: The study subject approaches a topic of cerebral pathology, namely the development of a pathological diagnosis that is as accurate as possible for the cerebral tumors and the metastases, using a new method that complements the optical microscopic examination and immunohistochemistry to correctly diagnose cerebral malignancies. Methods: Two-photon excitation (TPE) microscopy techniques are non-linear optical imaging methods that are gaining momentum in the investigation of fixed tissue sections, fresh tissue, or even for in vivo experiments. This method can be used for offering complementary information on the tissue architecture. We argue that using non-linear optical microscopy (which includes TPE microscopy) images collected on this category of brain tissue samples, can facilitate the interpretation of brain tumors and metastases, thus it is a solution worthy to explore. Scanning large areas of the lesions is important to avoid false-negative diagnoses because lesions are often non-uniform. Finding potential invasion sites is also restricted when using a narrow field-of-view imaging method. Results: Our study demonstrated that TPE microscopy can detect specific cellular features of cerebral tumors and metastases in good correlation with histopathological results. This approach can improve the accuracy of the cerebral tumors diagnosis and possibly other neoplasms. Conclusion: TPE microscopy is very important for future research because it could prevent the false or inadequate diagnosis of specific lesions and differentiate a primary tumor from a metastasis. The significance of the findings is attributable to cross-disciplinary cooperation and the constant use of a working standard across all of the experiments in this study.