bluuebunny/crossref_metadata_2025 huggingface dataset:
Cycling of Pt, Pd, and Rh Derived from Catalytic Converters: Potential Pathways and Biogeochemical Processes
Authors: Eliopoulos Ioannis-Porfyrios , Eliopoulos George , Sfendoni Theodora , Economou-Eliopoulos Maria
Published: July 2022

The present study is an integrated approach to the Pt, Pd, and Rh cycling derived from catalytic converters along highway roadsides of the Athens Basin, including their contents, the dispersed Pt- and Pd-bearing nano- and microparticles in dust and bioaccumulation in plants, aiming to assess the auto-catalyst-derived environmental impact to the large city of Athens and the potential human health risk. The determined mean values of 314 Pt, 510 Pd, and 23 Rh (all in μg/kg) in dust samples are much lower than the 2070 μg/kg Pt and 1985 μg/kg Pd contents in gully pots in the Katechaki peripheral highway and higher than the mean values of 230 Pt, 300 Pd, and 13 Rh (all in μg/kg) in the soil samples. With the exception of two samples from gully pots, from 51% to 70% of the samples (for the Pd and Pt, respectively) fall in the range from 100 to 400 μg/kg. The calculated accumulation factors showed means of 3.88 μg/kg Pd and 2.95 μg/kg Pt for plants and tree leaves, but any significant difference (t-test) is lacking, and they are much lower than those reported for roots of plants (literature data). Although the Pt, Pd, and Rh bioaccumulation factors for shoots of plants/crops are relatively low, the increasing number of cars with catalytic converters in Greece and the relatively high bioaccumulation in the food chain may highlight a potential risk for human health and ecosystems, and the need for special attention on their bioaccumulation and bioaccessibility on a global scale.
Mass Spectrometry-Based Peptide Profiling of Haemolymph from Pterostichus melas Exposed to Pendimethalin Herbicide
Authors: Aiello Donatella , Giglio Anita , Talarico Federica , Vommaro Maria Luigia , Tagarelli Antonio , Napoli Anna
Published: July 2022

Pendimethalin-based herbicides are used worldwide for pre-emergence selective control of annual grasses and weeds in croplands. The endurance of herbicides residues in the environment has an impact on the soil biodiversity and fertility, also affecting non-target species, including terrestrial invertebrates. Carabid beetles are known as natural pest control agents in the soil food web of agroecosystems, and feed on invertebrates and weed seeds. Here, a mass spectrometry untargeted profiling of haemolymph is used to investigate Pterostichus melas metabolic response after to pendimethalin-based herbicide exposure. Mass spectrometric data are examined with statistical approaches, such as principal component analysis, for possible correlation with biological effects. Those signals with high correlation are submitted to tandem mass spectrometry to identify the associated biomarker. The time course exposure showed many interesting findings, including a significant downregulation of related to immune and defense peptides (M-lycotoxin-Ls4a, Peptide hormone 1, Paralytic peptide 2, and Serine protease inhibitor 2). Overall, the observed peptide deregulations concur with the general mechanism of uptake and elimination of toxicants reported for Arthropods.
In Silico Analysis of PORD Mutations on the 3D Structure of P450 Oxidoreductase
Authors: Nurhafizuddin Muhammad , Azizi Aziemah , Ming Long Chiau , Shafqat Naeem
Published: July 2022

Cytochrome P450 oxidoreductase (POR) is a membrane-bound flavoprotein that helps in transferring electrons from its NADPH domain to all cytochrome P450 (CYP450) enzymes. Mutations in the POR gene could severely affect the metabolism of steroid hormones and the development of skeletal muscles, a condition known as Cytochrome P450 oxidoreductase deficiency (PORD). PORD is associated with clinical presentations of disorders of sex development, Antley and Bixler’s syndrome (ABS), as well as an abnormal steroid hormone profile. We have performed an in silico analysis of POR 3D X-ray protein crystal structure to study the effects of reported mutations on the POR enzyme structure. A total of 32 missense mutations were identified, from 170 PORD patients, and mapped on the 3D crystal structure of the POR enzyme. In addition, five of the missense mutations (R457H, A287P, D210G, Y181D and Y607C) were further selected for an in-depth in silico analysis to correlate the observed changes in POR protein structure with the clinical phenotypes observed in PORD patients. Overall, missense mutations found in the binding sites of POR cofactors could lead to a severe form of PORD, emphasizing the importance of POR cofactor binding domains in transferring electrons to the CYP450 enzyme family.
Three New Stigmatellin Derivatives Reveal Biosynthetic Insights of Its Side Chain Decoration
Authors: Okoth Dorothy A. , Hug Joachim J. , Garcia Ronald , Müller Rolf
Published: July 2022

Myxobacteria generate natural products with unique chemical structures, which not only feature remarkable biological functions, but also demonstrate unprecedented biosynthetic assembly strategies. The stigmatellins have been previously described as potent inhibitors of the mitochondrial and photosynthetic respiratory chain and originate from an unusual polyketide synthase assembly line. While previous biosynthetic investigations were focused on the formation of the 5,7-dimethoxy-8-hydroxychromone ring, side chain decoration of the hydrophobic alkenyl chain in position 2 was investigated less thoroughly. We report here the full structure elucidation, as well as cytotoxic and antimicrobial activities of three new stigmatellins isolated from the myxobacterium Vitiosangium cumulatum MCy10943T with side chain decorations distinct from previously characterized members of this compound family. The hydrophobic alkenyl chain in position 2 of the herein described stigmatellins feature a terminal carboxylic acid group (1), a methoxy group at C-12′ (2) or a vicinal diol (3). These findings provide further implications considering the side chain decoration of these aromatic myxobacterial polyketides and their underlying biosynthesis.
Super Early Scan of PSMA PET/CT in Evaluating Primary and Metastatic Lesions of Prostate Cancer
Authors: Mao Juanli , Gao Mingjun , Cui Bin , Zhang Yingying , Wang Xiaojiao , Liang Siyu , Zuo Changjing , Chen Peng , Dong Aisheng
Published: July 2022

68Ga-prostate specific membrane antigen (PSMA)-11 PET/CT has been widely used in the diagnosis of prostate cancer (PCa); however, the urine lead shielding resulting from the urinary metabolism of tracers may obstruct the detection of surrounding metastasis. In this research, the additive value of super early scanning in diagnosing primary lesions and metastasis in the pelvic cavity was evaluated. Firstly, the differentiation efficiency of 68Ga-PSMA-11 PET scanned at 3 min post-injection (min P.I.) was measured in PSMA-positive (22rv1 cells) and PSMA-negative (PC3 cells) model mice. Secondly, 106 patients were scanned at 3 min P.I. for the pelvic cavity and then scanned as a standard protocol at 45 min P.I. In the results, the differential diagnosis of PSMA expression was completely reflected as early as 3 min P.I. for mice models. For patients, when correlated with the Gleason score, the quantitative results of the super early scan displayed a comparable correlation coefficient with the routine scan. The target to bladder ratios increased from 1.44 ± 2.40 at 45 min to 10.10 ± 19.10 at 3 min (p < 0.001) for the primary lesions, and it increased from 0.99 ± 1.88 to 9.27 ± 23.03 for metastasis. Meanwhile, the target to background ratios increased from 2.21 ± 2.44 at 3 min to 19.13 ± 23.93 at 45 min (p < 0.001) for the primary lesions, and it increased from 1.68 ± 2.71 to 12.04 ± 18.73 (p < 0.001) for metastasis. In conclusion, super early scanning of 68Ga-PSMA-11 PET/CT added referable information for metastasis detection in order to avoid disturbing tracer activity in the urinary system.
Paramagnetic Properties and Moderately RapidConformational Dynamics in the Cobalt(II) Calix[4]arene Complex by NMR
Authors: Zapolotsky Eugeny Nikolaevich , Babailov Sergey Pavlovich , Kostin Gennadiy Alexandrovich
Published: July 2022

1H NMR measurements are reported for the CD2Cl2/CDCl3 solutions of the Co(II) calix[4]arenetetraphosphineoxide complex (I). Temperature dependences of the 1H NMR spectra of I have been analyzed using the line shape analysis, taking into account the temperature variation of paramagnetic chemical shifts, within the frame of the dynamic NMR method. Conformational dynamics of the 2:1 Co(II) calix[4]arene complexes was conditioned by the pinched cone ↔pinched cone interconversion of I (with activation Gibbs energy ΔG≠(298K) = 40 ± 3 kJ/mol. Due to substantial temperature dependence of paramagnetic shifts, complex I can be used as model compound for designing an NMR thermosensor reagent for local temperature monitoring.
Comparison of Different Fixation Methods for Combined Histological and Biomolecular Analysis of Fixed and Decalcified Bone Samples
Authors: Al-Maawi Sarah , Valenzuela Priscilia , Dohle Eva , Heselich Anja , Sader Robert , Ghanaati Shahram
Published: July 2022

The combination of histological and biomolecular analyses provides deep understanding of different biological processes and is of high interest for basic and applied research. However, the available analytical methods are still limited, especially when considering bone samples. This study compared different fixation media to identify a sufficient analytical method for the combination of histological, immuno-histological and biomolecular analyses of the same fixed, processed and paraffin embedded bone sample. Bone core biopsies of rats’ femurs were fixed in different media (RNAlater + formaldehyde (R + FFPE), methacarn (MFPE) or formaldehyde (FFPE)) for 1 week prior to decalcification by EDTA and further histological processing and paraffin embedding. Snap freezing (unfixed frozen tissue, UFT) and incubation in RNAlater were used as additional controls. After gaining the paraffin sections for histological and immunohistological analysis, the samples were deparaffined and RNA was isolated by a modified TRIZOL protocol. Subsequently, gene expression was evaluated using RT-qPCR. Comparable histo-morphological and immuno-histological results were evident in all paraffin embedded samples of MFPE, FFPE and R + FFPE. The isolated RNA in the group of MFPE showed a high concentration and high purity, which was comparable to the UFT and RNAlater groups. However, in the groups of FFPE and R + FFPE, the RNA quality and quantity were statistically significantly lower when compared to MFPE, UFT and RNAlater. RT-qPCR results showed a comparable outcome in the group of MFPE and UFT, whereas the groups of FFPE and R + FFPE did not result in a correctly amplified gene product. Sample fixation by means of methacarn is of high interest for clinical samples to allow a combination of histological, immunohistological and biomolecular analysis. The implementation of such evaluation method in clinical research may allow a deeper understanding of the processes of bone formation and regeneration.
Tailoring of AlAs/InAs/GaAs QDs Nanostructures via Capping Growth Rate
Authors: Ruiz Nazaret , Fernandez Daniel , Luna Esperanza , Stanojević Lazar , Ben Teresa , Flores Sara , Braza Verónica , Gallego-Carro Alejandro , Bárcena-González Guillermo , Yañez Andres , Ulloa José María , González David
Published: July 2022

The use of thin AlA capping layers (CLs) on InAs quantum dots (QDs) has recently received considerable attention due to improved photovoltaic performance in QD solar cells. However, there is little data on the structural changes that occur during capping and their relation to different growth conditions. In this work, we studied the effect of AlA capping growth rate (CGR) on the structural features of InAs QDs in terms of shape, size, density, and average content. As will be shown, there are notable differences in the characteristics of the QDs upon changing CGR. The Al distribution analysis in the CL around the QDs was revealed to be the key. On the one hand, for the lowest CGR, Al has a homogeneous distribution over the entire surface, but there is a large thickening of the CL on the sides of the QD. As a result, the QDs are lower, lenticular in shape, but richer in In. On the other hand, for the higher CGRs, Al accumulates preferentially around the QD but with a more uniform thickness, resulting in taller QDs, which progressively adopt a truncated pyramidal shape. Surprisingly, intermediate CGRs do not improve either of these behaviors, resulting in less enriched QDs.
Design and Simulation of Efficient SnS-Based Solar Cell Using Spiro-OMeTAD as Hole Transport Layer
Authors: Tiwari Pooja , Alotaibi Maged F. , Al-Hadeethi Yas , Srivastava Vaibhava , Arkook Bassim , Sadanand Sadanand , Lohia Pooja , Dwivedi Dilip Kumar , Umar Ahmad , Algadi Hassan , Baskoutas Sotirios
Published: July 2022

In the present paper, the theoretical investigation of the device structure ITO/CeO2/SnS/Spiro-OMeTAD/Mo of SnS-based solar cell has been performed. The aim of this work is to examine how the Spiro-OMeTAD HTL affects the performance of SnS-based heterostructure solar cell. Using SCAPS-1D simulation software, various parameters of SnS-based solar cell such as work function, series and shunt resistance and working temperature have been investigated. With the help of Spiro-OMeTAD, the suggested cell’s open-circuit voltage was increased to 344 mV. The use of Spiro-OMeTAD HTL in the SnS-based solar cell resulted in 14% efficiency increase, and the proposed heterojunction solar cell has 25.65% efficiency. The cell’s performance is determined by the carrier density and width of the CeO2 ETL (electron transport layer), SnS absorber layer and Spiro-OMeTAD HTL (hole transport layer). These data reveal that the Spiro-OMeTAD solar cells could have been a good HTL (hole transport layer) in regards to producing SnS-based heterojunction solar cell with high efficiency and reduced cost.
miR-218: A Stress-Responsive Epigenetic Modifier
Authors: Schell Grant , Roy Bhaskar , Prall Kevin , Dwivedi Yogesh
Published: July 2022

Understanding the epigenetic role of microRNAs (miRNAs) has been a critical development in the field of neuropsychiatry and in understanding their underlying pathophysiology. Abnormalities in miRNA expression are often seen as key to the pathogenesis of many stress-associated mental disorders, including major depressive disorder (MDD). Recent advances in omics biology have further contributed to this understanding and expanded the role of miRNAs in networking a diverse array of molecular pathways, which are essentially related to the stress adaptivity of a healthy brain. Studies have highlighted the role of many such miRNAs in causing maladaptive changes in the brain’s stress axis. One such miRNA is miR-218, which is debated as a critical candidate for increased stress susceptibility. miR-218 is expressed throughout the brain, notably in the hippocampus and prefrontal cortex (PFC). It is expressed at various levels through life stages, as seen by adolescent and adult animal models. Until now, a minimal number of studies have been conducted on human subjects to understand its role in stress-related abnormalities in brain circuits. However, several studies, including animal and cell-culture models, have been used to understand the impact of miR-218 on stress response and hypothalamic-pituitary-adrenal (HPA) axis function. So far, expression changes in this miRNA have been found to regulate signaling pathways such as glucocorticoid signaling, serotonergic signaling, and glutamatergic signaling. Recently, the developmental role of miR-218 has generated interest, given its increasing expression from adolescence to adulthood and targeting the Netrin-1/DCC signaling pathway. Since miR-218 expression affects neuronal development and plasticity, it is expected that a change in miR-218 expression levels over the course of development may negatively impact the process and make individuals stress-susceptible in adulthood. In this review, we describe the role of miR-218 in stress-induced neuropsychiatric conditions with an emphasis on stress-related disorders.
Nutrition and Physical Exercise in Women
Authors: Rocha-Rodrigues Sílvia , Afonso José , Sousa Mónica
Published: July 2022

While the benefits of nutrition and physical exercise are commonly studied separately, their concomitant integration has the potential to produce greater benefits in women than strategies focusing only on one or the other [...]
Gut Microbiota Characteristics of People with Obesity by Meta-Analysis of Existing Datasets
Authors: Gong Jinhua , Shen Yun , Zhang Hongcheng , Cao Man , Guo Muyun , He Jianquan , Zhang Bangzhou , Xiao Chuanxing
Published: July 2022

Obesity is a complex chronic, relapsing, progressive disease. Association studies have linked microbiome alterations with obesity and overweight. However, the results are not always consistent. An integrated analysis of 4282 fecal samples (2236 control (normal weight) group, 1152 overweight, and 894 simple obesity) was performed to identify obesity-associated microbial markers. Based on a random effects model and a fixed effects model, we calculated the odds ratios of the metrics, including bacterial alpha-diversity, beta-diversity, Bacteroidetes/Firmicutes ratio, common genera, and common pathways, between the simple obesity and control groups as well as the overweight and control groups. The random forest model was trained based on a single dataset at the genus level. Feature selection based on feature importance ranked by mean decrease accuracy and leave-one-out cross-validation was conducted to improve the predictive performance of the models. Chao1 and evenness possessed significant ORs higher than 1.0 between the obesity and control groups. Significant bacterial community differences were observed between the simple obesity and the control. The ratio of Bacteroidetes/Firmicutes was significantly higher in simple obesity patients. The relative abundance of Lachnoclostridium and Faecalitalea were higher in people with simple obesity, while 23 genera, including Christensenellaceae_R-7_group, Akkermansia, Alistipes, and Butyricimonas, etc., were significantly lower. The random forest model achieved a high accuracy (AUC = 0.83). The adenine and adenosine salvage pathway (PWY-6609) and the L-histidine degradation I pathway (HISDEG-PWY) were clustered in obese patients, while amino acid biosynthesis and degradation pathways (HISDEG-PWY, DAPLYSINESYN-PWY) were decreased. This study identified obesity microbial biomarkers, providing fertile targets for the management of obesity.
Cutoff Points of Waist Circumference for Predicting Incident Non-Alcoholic Fatty Liver Disease in Middle-Aged and Older Korean Adults
Authors: Lee Jun-Hyuk , Jeon Soyoung , Lee Hye Sun , Kwon Yu-Jin
Published: July 2022

This study aimed to determine the optimal cutoff value of waist circumference (WC) for predicting incident NAFLD. In this community-based prospective cohort study, we analyzed data from 5400 participants without NAFLD at baseline aged 40–69 years. NAFLD was defined as a NAFLD-liver fat score >−0.640. A Cox proportional hazards regression model was used to estimate the hazard ratio (HR) and 95% confidence interval (CI) for an association between body composition and NAFLD incidence. The predictive power of each body composition indicator was assessed by Harrell’s concordance index for Cox models. During a mean follow-up period of 12 years, there were 2366 new-onset NAFLD events. Compared with men with WC < 81 cm, the adjusted HR (95% CI) for incident NAFLD in those with WC ≥ 81 cm was 2.44 (2.23–2.67). Compared with women with WC < 78.5 cm, the adjusted HR (95% CI) for incident NAFLD in those with WC ≥ 78.5 cm was 2.54 (2.25–2.87). WC was the most significant risk factor for predicting incident NAFLD among body composition indicators in middle-aged and older Korean adults. The optimal WC cutoff point for predicting incident NALFD was 81 cm in men and 78.5 cm in women, which might assist in the early detection and prevention of NAFLD.
Quantification of Phytochemicals, Cellular Antioxidant Activities and Antiproliferative Activities of Raw and Roasted American Pistachios (Pistacia vera L.)
Authors: Yuan Wang , Zheng Bisheng , Li Tong , Liu Rui Hai
Published: July 2022

The consumption of pistachios has been linked to many potential health benefits. Phytochemicals in pistachios, including phenolics, vitamin E and carotenoids, have been considered to make contributions to the health benefits. The objectives of this study were (1) to explore the phytochemical profiles (total phenolics and total flavonoids, including both free and bound forms), selected phytochemicals, vitamin E and carotenoids of raw and roasted pistachios; (2) to determine total antioxidant activity and cellular antioxidant activity (CAA); and (3) to explore antiproliferative activities of pistachio extracts against human breast, liver and colon cancer cells in vitro. Both raw and roasted pistachios contained high total phenolics, at 479.9 ± 10.2 (raw) and 447.9 ± 9.4 (roasted) mg GAE/100 g, respectively, and high flavonoids, at 178.4 ± 10.6 (raw) and 144.1 ± 7.4 (roasted) mg GAE/100 g, respectively. The contributions of the free form to the total phenolics in pistachios were 82% (raw) and 84% (roasted), respectively, and the contributions of the free form to the total flavonoids in pistachios were 65% (raw) and 70% (roasted), respectively. Gentisic acid and catechin were the major phenolics in raw and roasted pistachios, respectively. Both raw and roasted pistachios had similar total antioxidant activity evaluated by Oxygen-Radical-Scavenging Capacity (ORAC) assay, at 7387.9 ± 467 (raw) and 7375.3 ± 602 (roasted) μmol TE/100 g, respectively. Both raw and roasted pistachio extracts exhibited cellular antioxidant activity inhibiting peroxyradical radical-induced oxidation, with CAA values of 77.39 ± 4.25 (wash) and 253.71 ± 19.18 (no wash) μmol QE/100 g of raw pistachios and 115.62 ± 3.02 (wash) and 216.76 ± 6.6 (no wash) μmol QE/100 g of roasted pistachios. Roasted pistachios contained more vitamin E when compared with raw pistachios, while raw pistachios contained more carotenoids than the roasted pistachios. Additionally, the free form of roasted pistachios extracts exhibited superior antiproliferation activity against HepG2, Caco-2 and MDA-MB-231 cancer cells in a dose-dependent manner, with EC50 34.73 ± 1.64, 36.66 ± 3.3 and 7.41 ± 0.82 mg per mL, respectively. These results provided new knowledge about the phytochemical profiles, antioxidant activity, cellular antioxidant activity and antiproliferative activity of raw and roasted pistachios.
Bolus Injection of Liraglutide Raises Plasma Glucose in Normal Rats by Activating Glucagon-like Peptide 1 Receptor in the Brain
Authors: Hsu Chia-Chen , Cheng Juei-Tang , Hsu Ping Hao , Li Yingxiao , Cheng Kai-Chun
Published: July 2022

Diabetes is commonly treated with glucagon-like peptide-1 receptor (GLP-1R) agonists including liraglutide and others. However, liraglutide was found to raise plasma glucose levels in normal rats. The current study aims to determine how liraglutide causes this contentious condition in rats, both normal and diabetic. An adrenalectomy was performed to investigate the relationship between steroid hormone and liraglutide. To investigate the effect of central liraglutide infusion on blood glucose in rats, rats were intracerebroventricularly administrated with liraglutide with or without HPA axis inhibitors such as berberine and dexamethasone. The results showed that a single injection of liraglutide caused a temporary increase in blood glucose in healthy rats. Another GLP-1R agonist, Exendin-4 (Ex-4), increased blood sugar in a manner similar to that of liraglutide. The effects of liraglutide were also blocked by guanethidine pretreatment and vanished in normal rats with adrenalectomy. Additionally, central infusion of liraglutide via intracerebroventricular (icv) injection into normal rats also causes a temporary increase in blood glucose that was blocked by GLP-1R antagonists or the inhibitors such as berberine and dexamethasone. Similarly, central liraglutide treatment causes temporary increases in plasma glucose, adrenocorticotropic hormone (ACTH), and cortisol levels, which were reversed by inhibitors for the hypothalamic-pituitary-adrenal (HPA) axis. In normal rats, the temporary glucose-increasing effect of liraglutide was gradually eliminated during consecutive daily treatments, indicating tolerance formation. Additionally, liraglutide and Ex-4 cross-tolerance was also discovered in normal rats. Liraglutide was more effective in diabetic rats than in normal rats in activating GLP-1R gene expression in the isolated adrenal gland. Interestingly, the effect of liraglutide on glycemic control varied depending on whether the rats were diabetic or not. In normal rats, bolus injection of liraglutide, such as Ex-4, may stimulate the HPA axis, resulting in hyperglycemia. The cross-tolerance of liraglutide and Ex-4 provided a novel perspective on GLP-1R activation.
Research Progress of Conjugated Nanomedicine for Cancer Treatment
Authors: Zhao Bin , Chen Sa , Hong Ye , Jia Liangliang , Zhou Ying , He Xinyu , Wang Ying , Tian Zhongmin , Yang Zhe , Gao Di
Published: July 2022

The conventional cancer therapeutic modalities include surgery, chemotherapy and radiotherapy. Although immunotherapy and targeted therapy are also widely used in cancer treatment, chemotherapy remains the cornerstone of tumor treatment. With the rapid development of nanotechnology, nanomedicine is believed to be an emerging field to further improve the efficacy of chemotherapy. Until now, there are more than 17 kinds of nanomedicine for cancer therapy approved globally. Thereinto, conjugated nanomedicine, as an important type of nanomedicine, can not only possess the targeted delivery of chemotherapeutics with great precision but also achieve controlled drug release to avoid adverse effects. Meanwhile, conjugated nanomedicine provides the platform for combining several different therapeutic approaches (chemotherapy, photothermal therapy, photodynamic therapy, thermodynamic therapy, immunotherapy, etc.) with the purpose of achieving synergistic effects during cancer treatment. Therefore, this review focuses on conjugated nanomedicine and its various applications in synergistic chemotherapy. Additionally, the further perspectives and challenges of the conjugated nanomedicine are also addressed, which clarifies the design direction of a new generation of conjugated nanomedicine and facilitates the translation of them from the bench to the bedside.
Impacts of Short-Term Grazing Intensity on the Plant Diversity and Ecosystem Function of Alpine Steppe on the Qinghai–Tibetan Plateau
Authors: Hao Xinghai , Yang Juejie , Dong Shikui , Shen Hao , He Fengcai , Zhi Yangliu , Kwaku Emmanuella A. , Tu Danjia , Dou Shengyun , Zhou Xueli , Yang Zhengrong
Published: July 2022

Livestock grazing is the primary land use of grasslands worldwide. Grazing has been asserted to alter grassland ecosystem functions, such as productivity, nutrient cycling, and biodiversity conservation. However, few studies have focused on the impact of grazing intensity on the ecosystem multifunctionality (EMF) of alpine grasslands. We conducted a field experiment of manipulating sheep grazing intensity effects on alpine steppe by surveying plant community characteristics and ecosystem functions. Our results showed that plant community composition was altered with increasing grazing intensity, and the dominant species shifted from grasses and sedges to forbs. EMF was the highest under no grazing (CK) and the lowest under heavy grazing (HG), but there was insignificant difference between CK and HG. HG significantly decreased some indicators that reflected nutrient cycling functions, such as soil available nitrogen, plant leaf nitrogen (PN) and phosphorus content (PP). Furthermore, plant diversity had strong correlations with SOC, total nitrogen (TN), and PN. The results could provide scientific bases for biodiversity conservation and sustainable grazing management of alpine steppe.
Highly Enhancing the Interfacial and Mechanical Properties of Basalt Fiber/Poly(phthalazinone ether nitrile ketone) Composite by Thermoplastic Sizing Agents with Different Structures
Authors: Jia Hang , Liu Cheng , Qiao Yue , Zhang Yu , Fan Kaiyuan , Zhang Manxia , Jian Xigao
Published: July 2022

The interfacial modification of basalt-fiber-reinforced polymer (BFRP) composites is an essential research field and many techniques have been developed to improve the adhesion between basalt fiber (BF) and the matrix. However, most studies were based on the matrixes of general plastics and epoxy resins. In this work, five different chain structures of thermoplastic sizing agents were used to improve the interfacial properties of unidirectional BF-reinforced soluble and high-temperature-resistant poly(phthalazinone ether nitrile ketone) (BF/PPENK) composites. DMA results showed that the poly(ether nitrile) (PEN)-sized BF/PPENK (BF-PEN/PPENK) composite exhibited the optimal interfacial performance, with a storage modulus (E′) and glass transition temperature (Tg) up to 50 GPa and 288 °C, respectively. Moreover, the tensile strength, compressive strength, flexural strength, and interlaminar shear strength of the BF-PEN/PPENK composite reached 778 MPa, 600 MPa, 1115 MPa and 57 MPa, respectively, and increased by 42%, 49%, 20% and 30% compared with the desized BF/PPENK composite. This study provides some suggestions for the design of sizing agents to modify the interface of BF and high-performance thermoplastic resin.
Equivalent Risk Indicators: VaR, TCE, and Beyond
Authors: Faroni Silvia , Le Courtois Olivier , Ostaszewski Krzysztof
Published: July 2022

While a lot of research concentrates on the respective merits of VaR and TCE, which are the two most classic risk indicators used by financial institutions, little has been written on the equivalence between such indicators. Further, TCE, despite its merits, may not be the most accurate indicator to take into account the nature of probability distribution tails. In this paper, we introduce a new risk indicator that extends TCE to take into account higher-order risks. We compare the quantiles of this indicator to the quantiles of VaR in a simple Pareto framework, and then in a generalized Pareto framework. We also examine equivalence results between the quantiles of high-order TCEs.
Nutritive Value of Cistus ladanifer L. for Ruminants: Seasonal Variation of Chemical Composition, Digestibility, and Antioxidant Activity of Its Morphological Fractions
Authors: Guerreiro Olinda , Soldado David , Dentinho Maria T. P. , Jerónimo Eliana
Published: July 2022

Cistus ladanifer has been used in ruminants feeding systems, but there is no information about the seasonal variation in chemical composition and nutritional value of each morphological fraction during its growth cycle. This study characterized the morphological fractions of C. ladanifer (leaves, stems, flower buds, flowers, and seed heads) throughout the year in chemical composition, in vitro digestibility, and antioxidant activity. The leaves were the morphological fraction more variable throughout the seasons, being characterized by low protein level (48.5–114 g/kg dry matter (DM)), moderate cell-wall content (240–267 g NDF/kg DM), high levels of condensed tannins (CT) (131–246 g/kg DM), and low in vitro organic matter digestibility (IVOMD) (29.3–34.3%). The distinctive chemical composition among various morphological fractions allowed the identification of four distinct groups, the first composed by stems, the second by the leaves, the third by the seed heads, and the last one by the flower buds and flowers. All the morphological fractions are sources of bioactive compounds namely phenolic compounds. Leaves are the fraction with higher nutritive value, especially when collected during winter and spring.
Satellite Network Task Deployment Method Based on SDN and ICN
Authors: Liu Zhiguo , Dong Xiaoqi , Wang Lin , Feng Jianxin , Pan Chengsheng , Li Yunqi
Published: July 2022

With the rapid development of 5G and the Internet of Things, satellite networks are emerging as an indispensable part of realizing wide-area coverage. The growth of the constellation of low-orbit satellites makes it possible to deploy edge computing services in satellite networks. This is, however, challenging due to the topological dynamics and limited resources of satellite networks. To improve the performance of edge computing in a satellite network, we propose a satellite network task deployment method based on SDN (software-defined network) and ICN (information-centric network). In this method, based on the full analysis of satellite network resources, a mission deployment model of a low-orbit satellite network is established. The genetic algorithm is then used to solve the proposed method. Experiments confirm that this method can effectively reduce the response delay of the tasks and the network traffic caused by task processing.
I Can Step Clearly Now, the TENS Is On: Transcutaneous Electric Nerve Stimulation Decreases Sensorimotor Uncertainty during Stepping Movements
Authors: Whittier Tyler T. , Weller Zachary D. , Fling Brett W.
Published: July 2022

Transcutaneous electric nerve stimulation (TENS) is a method of electrical stimulation that elicits activity in sensory nerves and leads to improvements in the clinical metrics of mobility. However, the underlying perceptual mechanisms leading to this improvement are unknown. The aim of this study was to apply a Bayesian inference model to understand how TENS impacts sensorimotor uncertainty during full body stepping movements. Thirty healthy adults visited the lab on two occasions and completed a motor learning protocol in virtual reality (VR) on both visits. Participants were randomly assigned to one of three groups: TENS on first visit only (TN), TENS on second visit only (NT), or a control group where TENS was not applied on either visit (NN). Using methods of Bayesian inference, we calculated the amount of uncertainty in the participants’ center of mass (CoM) position estimates on each visit. We found that groups TN and NT decreased the amount of uncertainty in the CoM position estimates in their second visit while group NN showed no difference. The least amount of uncertainty was seen in the TN group. These results suggest that TENS reduces the amount of uncertainty in sensory information, which may be a cause for the observed benefits with TENS.
Advanced Waveguide Based LOC Biosensors: A Minireview
Authors: Kanjwal Muzafar A. , Ghaferi Amal Al
Published: July 2022

This mini review features contemporary advances in mid-infrared (MIR) thin-film waveguide technology and on-chip photonics, promoting high-performance biosensing platforms. Supported by recent developments in MIR thin-film waveguides, it is expected that label-free assimilated MIR sensing platforms will soon supplement the current sensing technologies for biomedical diagnostics. The state-of-the-art shows that various types of waveguide material can be utilized for waveguide spectroscopic measurements in MIR. However, there are challenges to integrating these waveguide platforms with microfluidic/Lab-on-a-Chip (LOC) devices, due to poor light–material interactions. Graphene and its analogs have found many applications in microfluidic-based LOC devices, to address to this issue. Graphene-based materials possess a high conductivity, a large surface-to-volume ratio, a smaller and tunable bandgap, and allow easier sample loading; which is essential for acquiring precise electrochemical information. This work discusses advanced waveguide materials, their advantages, and disease diagnostics with MIR thin-film based waveguides. The incorporation of graphene into waveguides improves the light–graphene interaction, and photonic devices greatly benefit from graphene’s strong field-controlled optical response.
Predictive System Implementation to Improve the Accuracy of Urine Self-Diagnosis with Smartphones: Application of a Confusion Matrix-Based Learning Model through RGB Semiquantitative Analysis
Authors: Kim Seon-Chil , Cho Young-Sik
Published: July 2022

Urinalysis, an elementary chemical reaction-based method for analyzing color conversion factors, facilitates examination of pathological conditions in the human body. Recently, considerable urinalysis-centered research has been conducted on the analysis of urine dipstick colors using smartphone cameras; however, such methods have a drawback: the problem of reproducibility of accuracy through quantitative analysis. In this study, to solve this problem, the function values for each concentration of a range of analysis factors were implemented in an algorithm through urine dipstick RGB semi-quantitative color analysis to enable real-time results. Herein, pH, glucose, ketones, hemoglobin, bilirubin, protein (albumin), and nitrites were selected as analysis factors, and the accuracy levels of the existing equipment and the test application were compared and evaluated using artificial urine. In the semi-quantitative analysis, the red (R), green (G), and blue (B) characteristic values were analyzed by extracting the RGB characteristic values of the analysis factors for each concentration of artificial urine and obtaining linear function values. In addition, to improve the reproducibility of detection accuracy, the measurement value of the existing test equipment was set to an absolute value; using a machine-learning technique, the confusion matrix, we attempted to stabilize test results that vary with environment.
An Explainable Evolving Fuzzy Neural Network to Predict the k Barriers for Intrusion Detection Using a Wireless Sensor Network
Authors: de Campos Souza Paulo Vitor , Lughofer Edwin , Rodrigues Batista Huoston
Published: July 2022

Evolving fuzzy neural networks have the adaptive capacity to solve complex problems by interpreting them. This is due to the fact that this type of approach provides valuable insights that facilitate understanding the behavior of the problem being analyzed, because they can extract knowledge from a set of investigated data. Thus, this work proposes applying an evolving fuzzy neural network capable of solving data stream regression problems with considerable interpretability. The dataset is based on a necessary prediction of k barriers with wireless sensors to identify unauthorized persons entering a protected territory. Our method was empirically compared with state-of-the-art evolving methods, showing significantly lower RMSE values for separate test data sets and also lower accumulated mean absolute errors (MAEs) when evaluating the methods in a stream-based interleaved-predict-and-then-update procedure. In addition, the model could offer relevant information in terms of interpretable fuzzy rules, allowing an explainable evaluation of the regression problems contained in the data streams.
Digital Twin-Based Integrated Monitoring System: Korean Application Cases
Authors: Choi Sangsu , Woo Jungyub , Kim Jun , Lee Ju Yeon
Published: July 2022

A digital twin is a virtual model of a process, product, or service, which is one of the key technologies in the fourth industry. The pairing of the virtual and physical world allows analysis of data and monitoring of systems to head off problems before they occur. This paper presents a digital twin architecture and a system based on an interoperable data model. It explains how to build a digital twin for the integrated control monitoring using edge devices, data analytics, and realistic 3D visualization. The system allows continuous collaboration between field engineers for data gathering, designers for modeling 3D models, and layout engineers for layout changing by generating 3D digital twin models automatically. The system helps stakeholders focus on their respective roles to build digital twins. Examples applied to the Korean automotive parts makers are also introduced in this paper. The system can be easily used by small and medium-sized enterprises (SMEs) as well as large companies. Beyond simply watching the production site with CCTV, the production site can be intuitively managed based on the digital twin.
PLI-VINS: Visual-Inertial SLAM Based on Point-Line Feature Fusion in Indoor Environment
Authors: Zhao Zhangzhen , Song Tao , Xing Bin , Lei Yu , Wang Ziqin
Published: July 2022

In indoor low-texture environments, the point feature-based visual SLAM system has poor robustness and low trajectory accuracy. Therefore, we propose a visual inertial SLAM algorithm based on point-line feature fusion. Firstly, in order to improve the quality of the extracted line segment, a line segment extraction algorithm with adaptive threshold value is proposed. By constructing the adjacent matrix of the line segment and judging the direction of the line segment, it can decide whether to merge or eliminate other line segments. At the same time, geometric constraint line feature matching is considered to improve the efficiency of processing line features. Compared with the traditional algorithm, the processing efficiency of our proposed method is greatly improved. Then, point, line, and inertial data are effectively fused in a sliding window to achieve high-accuracy pose estimation. Finally, experiments on the EuRoC dataset show that the proposed PLI-VINS performs better than the traditional visual inertial SLAM system using point features and point line features.
Smart Insole Based on Flexi Force and Flex Sensor for Monitoring Different Body Postures
Authors: Lakho Rafique Ahmed , Abro Zamir Ahmed , Chen Jun , Min Rui
Published: July 2022

This study aims to fabricate smart insoles using wireless Flexi force and bend sensing technology. Polyvinyl chloride (PVC) film was chosen as the substrate to hold all the sensors. The developed smart insole has a three-layer structure (insole-PVC layer-fabric layer) and is calibrated in an isolation laboratory to evaluate its measurement performance. One male volunteer subject exhibited four different body postures, namely tree pose, forward-leaning, squatting, and forward folding pose. Changes in pressure distribution were considered to be similar for the forward, squat, and forward-folded positions. When subjects performed a full squat, the flex sensor exhibited maximum flexion during the squat position, and the flex sensor response against the squat pose was found to be higher by about 18.18% than in the forward lean, respectively. The tree pose has the highest error rate at the first metatarsal, about 18.6%, of which the maximum absolute relative error of the sensor is less than 5%. Plantar pressure distribution and body posture measurements were successfully validated using Flexi force and flex sensors embedded in the smart insole. The smart insole proposed in this research work has broader prospects for clinical application.
Hybrid Clustering and Routing Algorithm with Threshold-Based Data Collection for Heterogeneous Wireless Sensor Networks
Authors: Bilal Muhammad , Munir Ehsan Ullah , Alarfaj Fawaz Khaled
Published: July 2022

The concept of the internet of things (IoT) motivates us to connect bulk isolated heterogeneous devices to automate report generation without human interaction. Energy-efficient routing algorithms help to prolong the network lifetime of these energy-restricted smart devices that are connected by means of wireless sensor networks (WSNs). Current vendor-level advancements enable algorithm-level flexibility to design protocols to concurrently collect multiple application data while enforcing the reduction of energy expenditure to gain commercial success in the industrial stage. In this paper, we propose a hybrid clustering and routing algorithm with threshold-based data collection for heterogeneous wireless sensor networks. In our proposed model, homogeneous and heterogeneous nodes are deployed within specific regions. To reduce unnecessary data transmission, threshold-based conditions are presented to prevent unnecessary transmission when minor or no change is observed in the simulated and real-world applications. We further extend our proposed multi-hop model to achieve more network stability in dense and larger network areas. Our proposed model shows enhancement in terms of load balancing and end-to-end delay as compared to the other threshold-based energy-efficient routing protocols, such as the threshold-sensitive stable election protocol (TSEP), threshold distributed energy-efficient clustering (TDEEC), low-energy adaptive clustering hierarchy (LEACH), and energy-efficient sensor network (TEEN).
Digital Exclusion and the Structural Barriers to Safety Strategies among Men and Non-Binary Sex Workers Who Solicit Clients Online
Authors: Koenig Brett , Murphy Alka , Johnston Spencer , Pearson Jennie , Knight Rod , Gilbert Mark , Shannon Kate , Krüsi Andrea
Published: July 2022

Background: Evidence shows that online solicitation facilitates sex workers’ ability to mitigate the risk of workplace violence. However, little is known about how end-demand sex work criminalization and the regulation of online sex work sites shape men and non-binary sex workers’ ability to maintain their own safety while soliciting services online. Methods: We conducted 21 semi-structured interviews with men and non-binary sex workers in British Columbia between 2020–2021 and examined their ability to enact safety strategies online in the context of end-demand criminalization. Analysis drew on a structural determinants of health framework. Results: Most participants emphasized that sex work is not inherently dangerous and described how soliciting services online facilitated their ability to enact personal safety strategies and remain in control of client interactions. However, participants also described how end-demand criminalization, sex work stigma, and restrictive website policies compromise their ability to solicit services online and to enact safety strategies. Conclusions: Alongside calls to decriminalize sex work, these findings emphasize the need to normalize sex work as a form of labour, promote access to online solicitation among men and non-binary sex workers, and develop standards for online sex work platforms in partnership with sex workers that prioritize sex worker safety.
Surgical Ostioplasty of the Left Main Coronary Artery: An Alternative to Coronary Artery Bypass Grafting in the Treatment of Left Main Stem Isolated Ostial Stenosis—A Case Series
Authors: Perazzo Alvaro , de Oliveira Salerno Pedro Rafael Vieira , Ferreira Paulino Mariana , de Ataide Caliari Vitoria , Ribeiro Isabella Martins , Lorusso Roberto , de Carvalho Lima Ricardo , Salerno Pedro Rafael
Published: July 2022

This study aims to demonstrate the use of surgical ostioplasty of the left main coronary artery as an alternative technique to the conventional use of coronary artery bypass grafting for the treatment of left main stem-isolated ostial stenosis. From 2002 to 2021, five patients—three women (60%) and two men (40%)—presented with a history of angina pectoris and were diagnosed with isolated stenosis of the coronary ostia associated with normal distal coronary arteries. Pre-operative cardiac catheterization and echocardiography were performed to aid in the diagnosis. The patients were submitted to surgical ostioplasty with a posterior approach using a saphenous patch. There were no hospital deaths or myocardial infarctions during the post-operative period. The mean cardiopulmonary bypass time was 82 min (range, 70–95 min), and the mean aortic-clamp time was 62 min (range, 55–75 min). The average time of hospitalization was 6.2 days (range, 4–18 days). Patients with isolated stenosis of the coronary ostia associated with normal distal coronary arteries may be submitted to left main coronary artery ostioplasty as an alternative to coronary artery bypass grafting, the traditional surgical technique for this pathology.