bluuebunny/crossref_metadata_2025 huggingface dataset:
Identify successful performance of urban creativity indicators dimensions between 22 regions of Tehran Metropolitan: a comparative study
Authors: Rahimzadeh Azad , Jafarpour Ghalehteimouri Kamran
Published: June 2024

Creative cities are known as places for innovation and the growth of creativity, and in fact, the creative city is a favourable environment for training human creativity and attracting the creative class, providing an appropriate foundation for educating its residents' creativity. The aim of this study was to determine the extent to which Tehran's neighbourhoods have the characteristics of a creative city. The descriptive-analytical research method was used for this purpose. Documents are used to collect data in four dimensions: quality of life, human capital, social capital, and innovation. The statistical population of the study was the 22 districts of Tehran, and the research indicators included 28 indicators of the creative city in the form of 7 general dimensions. The data analysis method used was quantitative–qualitative. Using the multi-indicator ELECTRE-II method for ranking areas based on creative city indicators, districts 1 and 6 in Tehran are ranked first, and districts 17, 16, 10, and 9 are ranked last in terms of creativity. Finally, Tehran has the potential to become a creative city, but this requires the removal of existing flaws and obstacles, as well as well-codified and legal planning.
An integrated approach to strengthening maritime security: a case study of Gwadar Port of Pakistan
Authors: khan Asif , Shi Congmei , Ali Fayyaz
Published: June 2024

Gwadar Port is a vital component of Pakistan’s development and an essential element in the Belt and Road Initiative and the China–Pakistan Economic Corridor. Its completion holds the promise of economic growth for Baluchistan and Pakistan and provides a crucial trade route for land-locked Central Asian nations. Gwadar Port faces many significant challenges related to security due to the region’s poverty, instability, and particularly its separatist activities. To address this, a study has been conducted to determine how to improve maritime security at Gwadar Port. The study uses a literature review, comparative analysis, and field research to examine the factors that affect port security. Terrorist acts, particularly those planned by organizations such as the Baluchistan People’s Liberation Army, pose the greatest security risks. Additionally, the unique management approach of Gwadar Port presents other security challenges. This study on the experience of port management in China and Pakistan offers strategies for enhancing security management in Gwadar Port. These strategies include improved coordination between entities, increased security measures, and strengthened cooperation with the Chinese stakeholders engaged in port projects. Prioritizing the development of local communities and education is crucial to ensure the well-being of both port workers and the surrounding population. The conclusion stresses the significance of international cooperation, the sharing of information, and improved foresight to strengthen the security framework of Gwadar Port. Integrating best practices and refining approaches will improve safety management, safeguard the facilities, and foster their sustainable growth.
Time Varying Crustal Anisotropy at Whakaari/White Island Volcano
Authors: Mengesha D. Y. , Savage M. K. , Jolly A. D. , Ebinger C. J.
Published: June 2024

Whakaari/White Island has been the most active New Zealand volcano in the 21st century, producing small phreatic and phreatomagmatic eruptions, which are hard to predict. The most recent eruption occurred in 2019, tragically claiming the lives of 22 individuals and causing numerous injuries. We employed shear‐wave splitting analyses to investigate variations in anisotropy between 2018 and 2020, during quiescence, unrest, and the eruption. We examined spatial and temporal variations in 3,499 shear‐wave splitting and 2,656 Vp/Vs ratio measurements. Comparing shear‐wave splitting parameters from similar earthquake paths across different times indicates that the observed temporal changes are unlikely to result from variations in earthquake paths through media with spatial variability. Instead, these changes may stem from variations in anisotropy over time, likely caused by changes in crack alignment due to stress or varying fluid content.
A New Model of Electron Pitch Angle Distributions and Loss Timescales in the Earth's Radiation Belts
Authors: Glauert S. A. , Atkinson J. W. , Ross J. P. , Horne R. B.
Published: June 2024

As the number of satellites on orbit grows it is increasingly important to understand their operating environment. Physics‐based models can simulate the behavior of the Earth's radiation belts by solving a Fokker‐Planck equation. Three‐dimensional models use diffusion coefficients to represent the interactions between electromagnetic waves and the electrons. One‐dimensional radial diffusion models neglect the effects of energy diffusion and represent the losses due to the waves with a loss timescale. Both approaches may use pitch angle distributions (PADs) to create boundary conditions, to map observations from low to high equatorial pitch angles and to calculate phase‐space density from observations. We present a comprehensive set of consistent PADs and loss timescales for 2 ≤ L* ≤ 7, 100 keV ≤ E ≤ 5 MeV and all levels of geomagnetic activity determined by the Kp index. These are calculated from drift‐averaged diffusion coefficients that represent all the VLF waves that typically interact with radiation belt electrons and show good agreement with data. The contribution of individual waves is demonstrated; magnetosonic waves have little effect on loss timescales when lightning‐generated whistlers are present, and chorus waves contribute to loss even in low levels of geomagnetic activity. The PADs vary in shape depending on the dominant waves. When chorus is dominant the distributions have little activity dependence, unlike the corresponding loss timescales. Distributions peaked near 90° are formed by plasmaspheric hiss for L* ≤ 3 and E < 1 MeV, and by EMIC waves for L* > 3 and E > 1 MeV. When hiss dominates, increasing activity broadens the distribution but when EMIC waves dominate increasing activity narrows the distribution.
Longitudinal Recovery of Suspended Sediment Downstream of Large Dams in the US
Authors: Prajapati R. , Gardner J. , Pavelsky T. , Talchabhadel R.
Published: June 2024

Dams disrupt the natural flow of water and sediment along rivers. Reservoirs trap a significant amount of sediment, which substantially alters downstream hydrology, channel morphology, and sediment transport capacity. The longitudinal recovery of suspended sediment concentration (SSC) along rivers is potentially a new metric for estimating downstream responses to dams over space, rather than time, but is rarely quantified due to the lack of spatial SSC data. Satellites can estimate SSC along rivers where no field data exist and provide a high enough spatial resolution for assessing downstream recovery at the scale of tens to hundreds of kilometers. Here, we use a recently published database of spatially explicit SSC observations derived from Landsat to quantify if a river recovers or not, the SSC recovery percentage, and SSC recovery length downstream of large dams across the Contiguous United States (CONUS). Rivers recover SSC downstream of most dams (71%). The chance of a river recovering and the length of river required to recover SSC is associated primarily with the size of the reservoir (mean storage, km3) and the size of the river (mean discharge, m3/s). Rivers were more likely to recover SSC downstream of run‐of‐river, navigation dams compared to large storage or hydropower dams. Our results suggest that rivers typically recover suspended sediment downstream of dams, influenced by factors like dam storage, purpose, and river channel characteristics.
Atmospheric pCO2 Response to Stimulated Organic Carbon Export: Sensitivity Patterns and Timescales
Authors: Holzer Mark , DeVries Tim , Pasquier Benoît
Published: June 2024

The ocean's organic carbon export is a key control on atmospheric pCO2 and stimulating this export could potentially mitigate climate change. We use a data‐constrained model to calculate the sensitivity of atmospheric pCO2 to local changes in export using an adjoint approach. A perpetual enhancement of the biological pump's export by 0.1 PgC/yr could achieve a roughly 1% reduction in pCO2 at average sensitivity. The sensitivity varies roughly 5‐fold across different ocean regions and is proportional to the difference between the mean sequestration time τseq of regenerated carbon and the response time τpre of performed carbon, which is the reduction in the preformed carbon inventory per unit increase in local export production. Air‐sea CO2 disequilibrium modulates the geographic pattern of τpre, causing particularly high sensitivities (2–3 times the global mean) in the Antarctic Divergence region of the Southern Ocean.
Contribution to Environmental Perchlorate by Stratospheric Volcanic Eruptions
Authors: Kennedy Joshua A. , Cole‐Dai Jihong , Cox Thomas S. , Peterson Kari M. , Ferris David G.
Published: June 2024

Perchlorate in the environment originates from both natural and anthropogenic sources. A previous study of a 300‐year Greenland ice core perchlorate record found that anthropogenic impact on environmental perchlorate became significant starting around 1980, while natural formation is the only significant source of environmental perchlorate prior to that. The study also found increased perchlorate deposition in the Arctic following certain volcanic eruptions and suggested that at least some volcanic eruptions could enhance natural perchlorate production. Here we compare the perchlorate record with the volcanic record from sulfate in the same Greenland ice core and find that only stratospheric eruptions—large eruptions injecting volcanic substances directly into the stratosphere—enhance perchlorate production. No contribution to naturally formed perchlorate is detected from non‐stratospheric eruptions. The high‐resolution ice core perchlorate data are used to quantify contributions from volcanic eruptions, non‐volcanic natural processes, as well as from human activities during different periods. For the location in the Arctic in the perchlorate Pre‐Anthropogenic Era (1701–1979), the magnitude (0.26 μg m−2 yr−1 on average) of perchlorate produced during sporadic stratospheric eruptions is comparable to that (0.23 μg m−2 yr−1) produced by non‐volcanic natural processes. In the Anthropogenic Era (1980–2006), the magnitude of both the volcanic and non‐volcanic natural perchlorate production is similar to the enhancement (0.29 μg m−2 yr−1) by human activities.
Genetic influence on vascular smooth muscle cell apoptosis
Authors: McVey David G. , Andreadi Catherine , Gong Peng , Stanczyk Paulina J. , Solomon Charles U. , Turner Lenka , Yan Liu , Chen Runji , Cao Junjun , Nelson Christopher P. , Thompson John R. , Yu Haojie , Webb Tom R. , Samani Nilesh J. , Ye Shu
Published: June 2024

Vascular smooth muscle cell (VSMC) proliferation, migration, and apoptosis play important roles in many physiological processes and pathological conditions. To identify genetic influences on VSMC behavior, we measured these traits and undertook genome-wide association studies in primary umbilical artery-derived VSMCs from >2000 individuals. Although there were no genome-wide significant associations for VSMC proliferation or migration, genetic variants at two genomic loci (7p15.3 and 7q32.3) showed highly significant associations with VSMC apoptosis (P = 1.95 × 10−13 and P = 7.47 × 10−9, respectively). The lead variant at the 7p51.3 locus was associated with increased expression of the GSDME and PALS2 genes in VSMCs. Knockdown of GSDME or PALS2 in VSMCs attenuated apoptotic cell death. A protein co-immunoprecipitation assay indicated that GSDME complexed with PALS2. PALS2 knockdown attenuated activated caspase-3 and GSDME fragmentation, whilst GSDME knockdown also reduced activated caspase-3. These findings provide new insights into the genetic regulation of VSMC apoptosis, with potential utility for therapeutic development.
Deacetylated MDH1 and IDH1 aggravates PANoptosis in acute liver failure through endoplasmic reticulum stress signaling
Authors: Shi Chunxia , Wang Yukun , Guo Jin , Zhang Danmei , Zhang Yanqiong , Gong Zuojiong
Published: June 2024

Acute liver failure (ALF) is a disease with a high mortality rate and poor prognosis, whose pathogenesis is not fully understood. PANoptosis is a recently proposed mode of cell death characterized by pyroptosis, apoptosis, and necroptosis, but it cannot be explained by any of them alone. This study aims to explore the role of PANoptosis in ALF and the impact and mechanism of deacetylated malate dehydrogenase 1 (MDH1) and isocitrate dehydrogenase 1 (IDH1) on PANoptosis. Our results found that, compared with the control group, the cell viability in the lipopolysaccharide (LPS)/D-galactosamine (D-Gal) group decreased, lactate dehydrogenase (LDH) release increased, cell death increased, and the levels of PANoptosis-related molecules RIPK1, GSDMD, caspase-3, MLKL, IL-18, IL-1β increased, indicating that PANoptosis increased during ALF. Deacetylated MDH1 at K118 and IDH1 at K93 increased the expression of PANoptosis-related molecules RIPK1, GSDMD, caspase-3, MLKL, IL-18, and IL-1β in vivo and in vitro. The deacetylation weakened the inhibitory effect of histone deacetylase (HDAC) inhibitor ACY1215 on PANoptosis-related molecules, suggesting that deacetylated MDH1 at K118 and IDH1 at K93 aggravated PANoptosis during ALF. Deacetylated MDH1 at K118 and IDH1 at K93 also promoted the expression of endoplasmic reticulum stress-related molecules BIP, ATF6, XBP1, and CHOP in vivo and in vitro. The use of endoplasmic reticulum stress inhibitor 4-PBA weakened the promotion effect of deacetylated MDH1 K118 and IDH1 K93 on PANoptosis. The results suggested that deacetylated MDH1 at K118 and IDH1 at K93 may aggravate PANoptosis in ALF through endoplasmic reticulum stress signaling. In conclusion, deacetylated MDH1 and IDH1 may aggravate PANoptosis in ALF, and the mechanism may act through endoplasmic reticulum stress signaling.
Quantum coherence and interference of a single moiré exciton in nano-fabricated twisted monolayer semiconductor heterobilayers
Authors: Wang Haonan , Kim Heejun , Dong Duanfei , Shinokita Keisuke , Watanabe Kenji , Taniguchi Takashi , Matsuda Kazunari
Published: June 2024

The moiré potential serves as a periodic quantum confinement for optically generated excitons, creating spatially ordered zero-dimensional quantum systems. However, a broad emission spectrum resulting from inhomogeneity among moiré potentials hinders the investigation of their intrinsic properties. In this study, we demonstrated a method for the optical observation of quantum coherence and interference of a single moiré exciton in a twisted semiconducting heterobilayer beyond the diffraction limit of light. We observed a single and sharp photoluminescence peak from a single moiré exciton following nanofabrication. Our findings revealed the extended duration of quantum coherence in a single moiré exciton, persisting beyond 10 ps, and an accelerated decoherence process with increasing temperature and excitation power density. Moreover, quantum interference experiments revealed the coupling between moiré excitons in different moiré potential minima. The observed quantum coherence and interference of moiré exciton will facilitate potential applications of moiré quantum systems in quantum technologies.
Sub-100-fs energy transfer in coenzyme NADH is a coherent process assisted by a charge-transfer state
Authors: Jaiswal Vishal Kumar , Aranda Ruiz Daniel , Petropoulos Vasilis , Kabaciński Piotr , Montorsi Francesco , Uboldi Lorenzo , Ugolini Simone , Mukamel Shaul , Cerullo Giulio , Garavelli Marco , Santoro Fabrizio , Nenov Artur
Published: June 2024

Excitation energy transfer (EET) is a key photoinduced process in biological chromophoric assemblies. Here we investigate the factors which can drive EET into efficient ultrafast sub-ps regimes. We demonstrate how a coherent transport of electronic population could facilitate this in water solvated NADH coenzyme and uncover the role of an intermediate dark charge-transfer state. High temporal resolution ultrafast optical spectroscopy gives a 54±11 fs time constant for the EET process. Nonadiabatic quantum dynamical simulations computed through the time-evolution of multidimensional wavepackets suggest that the population transfer is mediated by photoexcited molecular vibrations due to strong coupling between the electronic states. The polar aqueous solvent environment leads to the active participation of a dark charge transfer state, accelerating the vibronically coherent EET process in favorably stacked conformers and solvent cavities. Our work demonstrates how the interplay of structural and environmental factors leads to diverse pathways for the EET process in flexible heterodimers and provides general insights relevant for coherent EET processes in stacked multichromophoric aggregates like DNA strands.
Rule-based omics mining reveals antimicrobial macrocyclic peptides against drug-resistant clinical isolates
Authors: Cheng Zhuo , He Bei-Bei , Lei Kangfan , Gao Ying , Shi Yuqi , Zhong Zheng , Liu Hongyan , Liu Runze , Zhang Haili , Wu Song , Zhang Wenxuan , Tang Xiaoyu , Li Yong-Xin
Published: June 2024

Antimicrobial resistance remains a significant global threat, driving up mortality rates worldwide. Ribosomally synthesized and post-translationally modified peptides have emerged as a promising source of novel peptide antibiotics due to their diverse chemical structures. Here, we report the discovery of new aminovinyl-(methyl)cysteine (Avi(Me)Cys)-containing peptide antibiotics through a synergistic approach combining biosynthetic rule-based omics mining and heterologous expression. We first bioinformatically identify 1172 RiPP biosynthetic gene clusters (BGCs) responsible for Avi(Me)Cys-containing peptides formation from a vast pool of over 50,000 bacterial genomes. Subsequently, we successfully establish the connection between three identified BGCs and the biosynthesis of five peptide antibiotics via biosynthetic rule-guided metabolic analysis. Notably, we discover a class V lanthipeptide, massatide A, which displays excellent activity against gram-positive pathogens, including drug-resistant clinical isolates like linezolid-resistantS. aureusand methicillin-resistantS. aureus, with a minimum inhibitory concentration of 0.25 μg/mL. The remarkable performance of massatide A in an animal infection model, coupled with a relatively low risk of resistance and favorable safety profile, positions it as a promising candidate for antibiotic development. Our study highlights the potential of Avi(Me)Cys-containing peptides in expanding the arsenal of antibiotics against multi-drug-resistant bacteria, offering promising drug leads in the ongoing battle against infectious diseases.
Exploring plant-derived phytochrome chaperone proteins for light-switchable transcriptional regulation in mammals
Authors: Kong Deqiang , Zhou Yang , Wei Yu , Wang Xinyi , Huang Qin , Gao Xianyun , Wan Hang , Liu Mengyao , Kang Liping , Yu Guiling , Yin Jianli , Guan Ningzi , Ye Haifeng
Published: June 2024

Synthetic biology applications require finely tuned gene expression, often mediated by synthetic transcription factors (sTFs) compatible with the human genome and transcriptional regulation mechanisms. While various DNA-binding and activation domains have been developed for different applications, advanced artificially controllable sTFs with improved regulatory capabilities are required for increasingly sophisticated applications. Here, in mammalian cells and mice, we validate the transactivator function and homo-/heterodimerization activity of the plant-derived phytochrome chaperone proteins, FHY1 and FHL. Our results demonstrate that FHY1/FHL form a photosensing transcriptional regulation complex (PTRC) through interaction with the phytochrome, ΔPhyA, that can toggle between active and inactive states through exposure to red or far-red light, respectively. Exploiting this capability, we develop a light-switchable platform that allows for orthogonal, modular, and tunable control of gene transcription, and incorporate it into a PTRC-controlled CRISPRa system (PTRCdcas) to modulate endogenous gene expression. We then integrate the PTRC with small molecule- or blue light-inducible regulatory modules to construct a variety of highly tunable systems that allow rapid and reversible control of transcriptional regulation in vitro and in vivo. Validation and deployment of these plant-derived phytochrome chaperone proteins in a PTRC platform have produced a versatile, powerful tool for advanced research and biomedical engineering applications.
Group-theoretic error mitigation enabled by classical shadows and symmetries
Authors: Zhao Andrew , Miyake Akimasa
Published: June 2024

Estimating expectation values is a key subroutine in quantum algorithms. Near-term implementations face two major challenges: a limited number of samples required to learn a large collection of observables, and the accumulation of errors in devices without quantum error correction. To address these challenges simultaneously, we develop a quantum error-mitigation strategy called symmetry-adjusted classical shadows, by adjusting classical-shadow tomography according to how symmetries are corrupted by device errors. As a concrete example, we highlight global U(1) symmetry, which manifests in fermions as particle number and in spins as total magnetization, and illustrate their group-theoretic unification with respective classical-shadow protocols. We establish rigorous sampling bounds under readout errors obeying minimal assumptions, and perform numerical experiments with a more comprehensive model of gate-level errors derived from existing quantum processors. Our results reveal symmetry-adjusted classical shadows as a low-cost strategy to mitigate errors from noisy quantum experiments in the ubiquitous presence of symmetry.
Chlorophyll a in lakes and streams of the United States (2005–2022)
Authors: Spaulding Sarah A. , Platt Lindsay R. C. , Murphy Jennifer C. , Covert Alex , Harvey Judson W.
Published: June 2024

The concentration of chlorophyll a in phytoplankton and periphyton represents the amount of algal biomass. We compiled an 18-year record (2005–2022) of pigment data from water bodies across the United States (US) to support efforts to develop process-based, machine learning, and remote sensing models for prediction of harmful algal blooms (HABs). To our knowledge, this dataset of nearly 84,000 sites and over 1,374,000 pigment measurements is the largest compilation of harmonized discrete, laboratory-extracted chlorophyll data for the US. These data were compiled from the Water Quality Portal (WQP) and previously unpublished U.S. Geological Survey’s National Water Quality Laboratory (NWQL) data. Data were harmonized for reporting units, pigment type, duplicate values, collection depth, site name, negative values, and some extreme values. Across the country, data show great variation by state in sampling frequency, distribution, and methods. Uses for such data include the calibration of models, calibration of field sensors, examination of relationship to nutrients and other drivers, evaluation of temporal trends, and other applications addressing local to national scale concerns.
Comprehensive Dataset on Electrical Load Profiles for Energy Community in Ireland
Authors: Trivedi Rohit , Bahloul Mohamed , Saif Aziz , Patra Sandipan , Khadem Shafi
Published: June 2024

This paper describes a comprehensive energy-related dataset, collected from residential electricity households within an energy community in Ireland, as part of StoreNet project. The data includes local weather parameters and per household power (W) and energy (Wh) components for various aspects such as active power consumption, PV generation, grid import and export, charging and discharging, and the state of charge of energy storage. Additionally, it provides weather data for the location at a 1-minute temporal resolution for the year 2020. The dataset has been validated by comparing measurements that should yield identical results to standard load profiles, with no significant inconsistencies discovered. Validation examples have also been given through the published articles where this dataset has been used to analyse peer-to-peer energy trading benefits for the energy community and decision-making support for aggregators. The dataset aids in understanding patterns in electrical load curves and the duty cycle of energy storage within an energy community. Furthermore, it can assist in comprehending the impact on distribution networks caused by distributed energy storage.
MEDUSA: Marine benthic Ecological Data from Underwater imagery Surveys of sub-Antarctic Crozet environments
Authors: Lelièvre Yann , Motreuil Sébastien , Specq Léa , Marschal Christian , Dubois Philippe , Wauters Lisa , Guéné Mathilde , Saucède Thomas
Published: June 2024

Inscribed on the UNESCO World Heritage list, the sub-Antarctic Crozet archipelago is located in a region facing significant environmental changes impacting a poorly known marine biodiversity. Underwater imagery constitutes a valuable non-invasive approach for gathering ecological data and improving our knowledge of ecosystems’ vulnerability. We here compiled two datasets, encompassing 17 video-imagery surveys of Crozet nearshore environments conducted in 2021 and 2022 at two sites of Ile de la Possession: Baie du Marin and Crique du Sphinx. Faunal abundance and algal cover data related to each survey are also provided. A total of 755 images were analysed, comprising 52 faunal and 14 algal taxa identified in 2021, as well as 45 faunal and 14 algal taxa identified in 2022. Video-transects were performed in shallow waters by scuba divers using a GoPro®HERO7 multiple camera set-up, and in deeper waters using a remotely operated vehicle. These data provide a first baseline for biodiversity and ecosystem studies, and for monitoring the long-term dynamics of Crozet benthic habitats facing natural and anthropogenic disturbances.
Targeting the ZMIZ1-Notch1 signaling axis for the treatment of tongue squamous cell carcinoma
Authors: Pang Yunqing , Sun Yunjie , Wu Yuyan , Li Jiamin , Qin Pingchuan , Guo Shanchuan , Zhou Wenlian , Chen Jian , Wang Jing
Published: June 2024

Zinc finger MIZ-type containing 1 (ZMIZ1) is a transcriptional coactivator related to the protein inhibitors of activated STATs (PIAS) family. Mounting evidence suggests that ZMIZ1 plays a crucial role in the occurrence and development of cancers. The function of ZMIZ1 in tongue squamous cell carcinoma (TSCC) and the mechanisms underpinning its role in this disease have not been fully clarified. We performed qualitative ZMIZ1 protein expression analyses using immunohistochemistry in 20 patient-derived, paraffin-embedded TSCC tissue sections. We used RNAi to knock down ZMIZ1 expression in the CAL-27 TSCC cell line and quantified the impact of ZMIZ1 knock down on proliferation, migration and apoptosis via CCK-8, scratch assay and flow cytometry, respectively. We used qRT-PCR and western blotting to investigate the role of ZMIZ1 in this cell line. Finally, we established a model of lung metastasis in nude mice to replicate the in vitro results. ZMIZ1 protein was significantly more abundant in TSCC case tissue samples. ZMIZ1 knockdown reduced the invasion and metastases of TSCC tumor cells and promoted apoptosis. ZMIZ1 knockdown was associated with the down-regulation of Notch signaling pathway related factors Jagged1 and Notch1, and invasion and metastasis related factors MKP-1, SSBP2 and MMP7 in vitro and in vivo, at the mRNA level. In vitro and in vivo data suggest that knock down of ZMIZ1 may inhibit TSCC invasion and metastasis by modulating Notch signaling. ZMIZ1 inhibition may therefore represent a new therapeutic target for TSCC.
Effect of salt-alkali stress on seed germination of the halophyte Halostachys caspica
Authors: Zhang Rui , Zhang Huizhen , Wang Lai , Zeng Youling
Published: June 2024

The increasing global phenomenon of soil salinization has prompted heightened interest in the physiological ecology of plant salt and alkali tolerance. Halostachys caspica belonging to Amaranthaceae, an exceptionally salt-tolerant halophyte, is widely distributed in the arid and saline-alkali regions of Xinjiang, in Northwest China. Soil salinization and alkalinization frequently co-occur in nature, but very few studies focus on the interactive effects of various salt and alkali stress on plants. In this study, the impacts on the H. caspica seed germination, germination recovery and seedling growth were investigated under the salt and alkali stress. The results showed that the seed germination percentage was not significantly reduced at low salinity at pH 5.30–9.60, but decreased with elevated salt concentration and pH. Immediately after, salt was removed, ungerminated seeds under high salt concentration treatment exhibited a higher recovery germination percentage, indicating seed germination of H. caspica was inhibited under the condition of high salt-alkali stress. Stepwise regression analysis indicated that, at the same salt concentrations, alkaline salts exerted a more severe inhibition on seed germination, compared to neutral salts. The detrimental effects of salinity or high pH alone were less serious than their combination. Salt concentration, pH value, and their interactions had inhibitory effects on seed germination, with salinity being the decisive factor, while pH played a secondary role in salt-alkali mixed stress.
Biomechanical analysis of adjacent segments after correction surgery for adult idiopathic scoliosis: a finite element analysis
Authors: Wang Dong-hai , Wu Dan-ni , Xin Da-qi , Shi Qin , Wang Wen-xuan , Xing Wen-hua , Yang Hui-lin
Published: June 2024

The biomechanical aspects of adjacent segment degeneration after Adult Idiopathic Scoliosis (AdIS) corrective surgery involving postoperative changes in motion and stress of adjacent segments have yet to be investigated. The objective of this study was to evaluate the biomechanical effects of corrective surgery on adjacent segments in adult idiopathic scoliosis by finite element analysis. Based on computed tomography data of the consecutive spine from T1–S1 of a 28-year-old male patient with adult idiopathic scoliosis, a three-dimensional finite element model was established to simulate the biomechanics. Two posterior long-segment fixation and fusion operations were designed: Strategy A, pedicle screws implanted in all segments of both sides, and Strategy B, alternate screws instrumentation on both sides. The range of motion (ROM), Maximum von Mises stress value of intervertebral disc (IVD), and Maximum von Mises stress of the facet joint (FJ) at the fixation adjacent segment were calculated and compared with data of the preoperative AdIS model. Corrective surgery decreased the IVD on the adjacent segments, increased the FJ on the adjacent segments, and decreased the ROM of the adjacent segments. A greater decrease of Maximum von Mises stress was observed on the distal adjacent segment compared with the proximal adjacent segment. The decrease of Maximum von Mises stress and increment of Maximum von Mises stress on adjacent FJ in strategy B was greater than that in strategy A. Under the six operation modes, the change of the Maximum von Mises stress on the adjacent IVD and FJ was significant. The decrease in ROM in the proximal adjacent segment was greater than that of the distal adjacent segment, and the decrease of ROM in strategy A was greater than that in strategy B. This study clarified the biomechanical characteristics of adjacent segments after AdIS corrective surgery, and further biomechanical analysis of two different posterior pedicle screw placement schemes by finite element method. Our study provides a theoretical basis for the pathogenesis, prevention, and treatment of adjacent segment degeneration after corrective surgery for AdIS.
Effects of weight loss intervention on anxiety, depression and quality of life in women with severe obesity and polycystic ovary syndrome
Authors: Kataoka Josefin , Olsson Marie , Lindgren Eva , Larsson Ingrid , Schmidt Johanna , Benrick Anna , Stener-Victorin Elisabet
Published: June 2024

Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women that is associated with an increased risk of anxiety and depression and with a lower health-related quality of life (HRQoL). PCOS is closely associated with obesity, which per se can lead to symptoms of anxiety and depression and lower HRQoL. The first-line treatment for PCOS is weight loss through lifestyle intervention, which has been shown to improve all symptoms of the syndrome. The aim of this study was to investigate symptoms of anxiety and depression and HRQoL in women with severe obesity (BMI ≥ 35) with and without PCOS, and to evaluate the effect of a one-year structured weight loss intervention. A total of 246 women with severe obesity (PCOS n = 63, non-PCOS n = 183) were included. The comprehensive psychopathological rating scale self-rating scale for affective symptoms (CPRS-S-A) and the short form-36 (SF-36) were used to assess symptoms of anxiety and depression and HRQoL. In total 72 women of the 246 women with severe obesity completed a one-year weight loss programme and were followed up and compared with baseline data. In women with severe obesity, there were no differences in symptoms of anxiety and depression and HRQoL between women with and without PCOS at baseline. Clinically relevant anxiety symptoms were present in 71.3% (PCOS) and 65.6% (non-PCOS), and depression symptoms were present in 56.4% (PCOS) and 52.2% (non-PCOS). Significant weight loss improved physical HRQoL in all women, but reduced symptoms of anxiety and depression only in women without PCOS. There were no differences when comparing the changes between the groups. Women with severe obesity are severely affected by symptoms of anxiety and depression, independent of PCOS. Weight loss improved symptoms of anxiety and depression in women without PCOS, but there were no differences between groups in change from baseline to follow-up.

Trial registration number: Clinical trial.gov: NCT01319162, March 18, 2011. Date of registration and enrolment of the first subject September 2011.
Induction and antiviral activity of ferret myxovirus resistance (Mx) protein 1 against influenza A viruses
Authors: Farrukee Rubaiyea , Schwab Lara S. U. , Barnes James B. , Brooks Andrew G. , Londrigan Sarah L. , Hartmann Gunther , Zillinger Thomas , Reading Patrick C.
Published: June 2024

Myxovirus resistance (Mx) proteins are products of interferon stimulated genes (ISGs) and Mx proteins of different species have been reported to mediate antiviral activity against a number of viruses, including influenza A viruses (IAV). Ferrets are widely considered to represent the ‘gold standard’ small animal model for studying pathogenesis and immunity to human IAV infections, however little is known regarding the antiviral activity of ferret Mx proteins. Herein, we report induction of ferret (f)Mx1/2 in a ferret lung cell line and in airway tissues from IAV-infected ferrets, noting that fMx1 was induced to higher levels that fMx2 both in vitro and in vivo. Overexpression confirmed cytoplasmic expression of fMx1 as well as its ability to inhibit infection and replication of IAV, noting that this antiviral effect of fMx1was modest when compared to cells overexpressing either human MxA or mouse Mx1. Together, these studies provide the first insights regarding the role of fMx1 in cell innate antiviral immunity to influenza viruses. Understanding similarities and differences in the antiviral activities of human and ferret ISGs provides critical context for evaluating results when studying human IAV infections in the ferret model.
Taxonomic review, palaeoecological, and palaeobiogeographical significances of Campanian Tethyan oysters from the North Eastern Desert, Egypt
Authors: Moneer El Sayed M. , Bazeen Youssef S. , El-Sheikh Islam , Samir Ahmed
Published: June 2024

The Late Cretaceous was a time of high eustatic sea level that enabled extensive epicontinental seaways and carbonate platforms across the Tethyan Realm, providing favorable habitats for oyster communities to flourish. This study focuses on the Campanian Tethyan oysters from the North Eastern Desert of Egypt regarding taxonomy, palaeoecology, and palaeobiogeography. Three oyster species, Nicaisolopha nicaisei (Coquand, 1862), Pycnodonte (Phygraea) vesicularis (Lamarck, 1806), and Ambigostrea bretoni (Thomas and Peron, 1891), were identified from the Campanian succession in two studied sections. The sampled specimens of the genus Nicaisolopha have undergone a systematic palaeontological revision. As a result, N. tissoti (Thomas and Peron, 1891) is considered herein a junior synonym of N. nicaisei (Coquand, 1862). Palaeobiogeographically, the likely primary migration pattern of the studied oysters suggests an east–west trend along the Southern Tethys margin. All identified oysters in this study exhibit a Tethyan affinity and are primarily abundant in two main provinces: the Southern Tethys and the Western Tethys. The macrofaunal contents are categorized into two fossil associations: the Nicaisolopha nicaisei association of the middle-late Campanian age and the Pycnodonte vesicularis association of the late Campanian age. These macrofaunal associations indicate a deepening trend during the middle-late Campanian age, suggesting a transition from shallow inner neritic to middle neritic environments. Additionally, it is observed that Pycnodonteinae tend to grow larger under eutrophic conditions, low-energy environments, and nutrient-rich waters with high carbonate contents.
Ex vivo investigation on the effect of minimally invasive endodontic treatment on vertical root fracture resistance and crack formation
Authors: Rathke Andreas , Frehse Henry , Bechtold Maria
Published: June 2024

The evidence base on minimally invasive endodontic (MIE) treatment is limited. This study investigated the influence of MIE shaping on vertical root fracture (VRF) resistance and crack formation of root canal filled teeth. Human maxillary central incisors were randomized into six groups (n = 18, power = 0.9) and embedded in acrylic blocks with artificial periodontal ligaments. The root canals were either instrumented to size #40 and 0.04 taper (+MIE) or enlarged to ISO size #80 (−MIE). The canals were filled with cement-based (C) or adhesive resin-based (A) sealers in single-cone technique. The controls received no treatment or were left unfilled. After chewing simulation (staircase method, 25–150 N, 120,000×), the crack formation on the root surface was analyzed using stereomicroscope/digital imaging and classified (no defect, craze line, vertical crack, horizontal crack). Subsequently, the samples were loaded until fracture. The incidence of defects (56% vertical cracks) was not significantly different between the groups (p ≥ 0.077). VRF resistance was significantly higher in untreated teeth than in +MIE/C (p = 0.020) but did not significantly differ between the other groups (p ≥ 0.068). Minimal canal shaping did not reduce the risk of vertical root fracture and defects of root canal filled teeth.
Active offset-frequency control of optical frequency comb via sum-frequency mixing of passively mode-locked laser and continuous-wave laser
Authors: Jeong Hansol , Kim Deok Woo , Kim Hyunhak , Cha Myoungsik , Moon Han Seb
Published: June 2024

We propose a method for actively controlling the frequency of an optical frequency comb (OFC) using sum-frequency generation (SFG) with a nonlinear crystal. For the first time, OFC generation was experimentally demonstrated via sum-frequency mixing of a narrowband continuous wave (CW) laser and a passively mode-locked fiber laser. By adjusting the optical frequency of the CW laser, we successfully controlled the offset-frequency of the SFG-OFC, which was mapped from the OFC of the pulse pump laser. Furthermore, by comparing the spectral widths of the SFG-OFC modes generated from two CW lasers with different spectral widths, we confirmed that the spectral characteristics of the SFG-OFC modes depended on the spectral features of the CW laser.
The association of thyroid autoimmunity with ovarian reserve in women with type 1 diabetes with and without polycystic ovary syndrome
Authors: Łebkowska Agnieszka , Krentowska Anna , Adamska Agnieszka , Uruska Aleksandra , Rogowicz-Frontczak Anita , Araszkiewicz Aleksandra , Ożegowska Katarzyna , Leśniewska Monika , Sowa Paweł , Wender-Ożegowska Ewa , Zozulińska-Ziółkiewicz Dorota , Kowalska Irina
Published: June 2024

The aim of the study was to investigate the relation between thyroid autoimmunity (TAI), reflected as the presence of thyroid peroxidase antibodies (TPOAb), and parameters of ovarian reserve in women with type 1 diabetes (T1DM) and polycystic ovary syndrome (PCOS). We studied 83 euthyroid women with T1DM (age – 26 ± 5 years, BMI – 24 ± 3 kg/m2) – 12 with PCOS and positive TPOAb (PCOS + TPOAb), 29 with PCOS with negative TPOAb (PCOS + noTPOAb), 18 without PCOS with positive TPOAb (noPCOS + TPOAb), 24 without PCOS with negative TPOAb (noPCOS + noTPOAb). Serum concentrations of anti-Müllerian hormone (AMH), sex hormones, TSH, thyroid hormones and TPOAb were assessed. The prevalence of TAI was comparable between PCOS and noPCOS. We did not observe differences in hormonal profile or AMH concentration between two PCOS groups—PCOS + TPOAb and PCOS + noTPOAb (p > 0.05). Women with PCOS + TPOAb had lower FSH concentration and higher LH/FSH index than noPCOS + noTPOAb (p = 0.027; p = 0.019, respectively). Moreover, PCOS + TPOAb had lower oestradiol level than noPCOS + TPOAb (p = 0.041). AMH concentration was higher in both groups with PCOS, independent of TPOAb presence, than in noPCOS + noTPOAb (both p < 0.001). The presence of positive TPOAb titre was not related to the studied parameters of ovarian reserve – AMH and ovarian follicle number. In multiple linear regression analysis, the only significant predictor of AMH in the whole studied group with T1DM was total daily insulin dose U/kg (β = − 0.264; p = 0.022). The presence of TAI did not affect the hormonal profile or ovarian reserve in women with T1DM with and without PCOS.
HTR2B as a novel biomarker of chronic obstructive pulmonary disease with lung squamous cell carcinoma
Authors: Li Yue , Wang Yu , Wu Ruhao , Li Pengfei , Cheng Zhe
Published: June 2024

Chronic obstructive pulmonary disease (COPD) is often associated with lung squamous cell carcinoma (LUSC), which has the same etiology (smoking, inflammation, oxidative stress, microenvironmental changes, and genetics). Smoking, inflammation, and airway remodeling are the most important and classical mechanisms of COPD comorbidity in LUSC patients. Cancer can occur during repeated airway damage and repair (airway remodeling). Changes in the inflammatory and immune microenvironments, which can cause malignant transformation of some cells, are currently being revealed in both LUSC and COPD patients. We obtained the GSE76925 dataset from the Gene Expression Omnibus database. Screening for possible COPD biomarkers was performed using the LASSO regression model and a random forest classifier. The compositional patterns of the immune cell fraction in COPD patients were determined using CIBERSORT. HTR2B expression was analyzed using validation datasets (GSE47460, GSE106986, and GSE1650). HTR2B expression in COPD cell models was determined via real-time quantitative PCR. Epithelial–mesenchymal transition (EMT) marker expression levels were determined after knocking down or overexpressing HTR2B. HTR2B function and mechanism in LUSC were analyzed with the Kaplan‒Meier plotter database. HTR2B expression was inhibited to detect changes in LUSC cell proliferation. A total of 1082 differentially expressed genes (DEGs) were identified in the GSE76925 dataset (371 genes were significantly upregulated, and 711 genes were significantly downregulated). Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the DEGs were mainly enriched in the p53 signaling and β-alanine metabolism pathways. Gene Ontology enrichment analysis indicated that the DEGs were largely related to transcription initiation from the RNA polymerase I promoter and to the regulation of mononuclear cell proliferation. The LASSO regression model and random forest classifier results revealed that HTR2B, DPYS, FRY, and CD19 were key COPD genes. Immune cell infiltration analysis indicated that these genes were closely associated with immune cells. Analysis of the validation sets suggested that HTR2B was upregulated in COPD patients. HTR2B was significantly upregulated in COPD cell models, and its upregulation was associated with increased EMT marker expression. Compared with that in bronchial epithelial cells, HTR2B expression was upregulated in LUSC cells, and inhibiting HTR2B expression led to the inhibition of LUSC cell proliferation. In conclusions, HTR2B might be a new biomarker and therapeutic target in COPD patients with LUSC.
The interplay of group size and flow velocity modulates fish exploratory behaviour
Authors: Mozzi Gloria , Nyqvist Daniel , Ashraf Muhammad Usama , Comoglio Claudio , Domenici Paolo , Schumann Sophia , Manes Costantino
Published: June 2024

Social facilitation is a well-known phenomenon where the presence of organisms belonging to the same species enhances an individual organism’s performance in a specific task. As far as fishes are concerned, most studies on social facilitation have been conducted in standing-water conditions. However, for riverine species, fish are most commonly located in moving waters, and the effects of hydrodynamics on social facilitation remain largely unknown. To bridge this knowledge gap, we designed and performed flume experiments where the behaviour of wild juvenile Italian riffle dace (Telestes muticellus) in varying group sizes and at different mean flow velocities, was studied. An artificial intelligence (AI) deep learning algorithm was developed and employed to track fish positions in time and subsequently assess their exploration, swimming activity, and space use. Results indicate that energy-saving strategies dictated space use in flowing waters regardless of group size. Instead, exploration and swimming activity increased by increasing group size, but the magnitude of this enhancement (which quantifies social facilitation) was modulated by flow velocity. These results have implications for how future research efforts should be designed to understand the social dynamics of riverine fish populations, which can no longer ignore the contribution of hydrodynamics.