bluuebunny/crossref_metadata_2025 huggingface dataset:
Autoeficacia e intención emprendedora en estudiantes pertenecientes a universidades privadas de Lima Metropolitana
Authors:   , Benito Sulca Yasmin Carolina , Urbina Bautista Dharly Yardel
Published: October 2023

El objetivo del presente estudio fue determinar la relación entre la autoeficacia e intención emprendedora en estudiantes de universidades privadas de Lima Metropolitana, así como identificar diferencias en estas variables según el tipo de carrera. La muestra estuvo conformada por 143 universitarios entre 18 y 25 años, donde 31.5% fueron hombres y 68.5% mujeres; 57% de los estudiantes pertenecen a la facultad de humanidades y 43% a la facultad de negocios. Se aplicó la validación peruana de la Escala de Autoeficacia General (EAG) y la adaptación colombiana del Cuestionario de Intención Emprendedora (CIE). Se obtuvo una relación positiva y significativa entre la autoeficacia y dos dimensiones de la escala de CIE: autoeficacia emprendedora (r= .43) e intención emprendedora (r= .41). Además, se encontró diferencias en las dimensiones actitud hacia la conducta emprendedora, autoeficacia emprendedora e intención emprendedora, obteniendo los estudiantes de carreras de negocios puntajes significativamente más altos que los estudiantes de humanidades, aunque dichas diferencias fueron pequeñas. Según estos hallazgos, se confirma la relación entre la autoeficacia e intención emprendedora, contribuyendo así en la toma de decisión por parte del estudiante para incursionar en un negocio propio, desde la formulación de la idea de creación hasta el proceso para llevarlo a cabo.
Ocurrencia de complicaciones en pacientes adultos con enfermedad inflamatoria intestinal e infección por Clostridiodes difficile: Una revisión sistemática
Authors:   , Caro Valera Luis Augusto , Sánchez Rojas Ryan Joshua
Published: October 2023

Introducción: La enfermedad inflamatoria intestinal (EII) es una patología crónica e idiopática, que presenta alta comorbilidad con infecciones por Clostridioides difficile (CDI). No se conoce exactamente cuál es el efecto de esta comorbilidad sobre los desenlaces hospitalarios en pacientes con EII; por lo que el objetivo fue evaluar la magnitud de asociación entre CDI y tiempo de hospitalización, readmisión luego de evento, número de readmisiones, visitas a emergencia, obstrucción intestinal, colectomías, cirugías abdominales, y muerte. Métodos: Revisión sistemática, en base al Cochrane Handbook, la población fueron personas con EII, ya sea enfermedad de Crohn o Colitis ulcerativa; la exposición fue CDI, y los desenlaces mencionados en el objetivo. La búsqueda se hizo mediante MEDLINE/Pubmed, LILACS/Scielo, Cochrane database, Web of Science, Scopus, Google Scholar y Google Académico. Para los desenlaces numéricos se extrajeron medias con desviación estándar, o fueron estimadas a partir de las medianas reportadas. Para los desenlaces dicotómicos se extrajo número de eventos y total para expuestos y no expuestos. Para los desenlaces que tuvieron 3 o más estudios, se hicieron meta-análisis con modelos aleatorios y varianza inversa, calculando ya sea diferencia de medias u odds ratios (OR). La calidad de estudios se evaluó con la escala Newcastle Ottawa, y sesgos de publicación con Funnel Plots. Resultados: De los 1386 artículos encontrados, 25 cumplieron con los criterios de selección. De este modo, los pacientes con CDI tienen 3.56 más días de tiempo de hospitalización (IC95%: +1.73; +5.40), 66% más probabilidad de ser readmitidos (OR 1.66; IC95%: 1.17; 2.34), 5.49 veces la probabilidad de fallecer que los que no tuvieron la infección (OR 5.49; IC 95%: 4.2; 7.18). Así mismo, estos pacientes mostraron una tendencia mayor a ser hospitalizados, colectomizados y operados del abdomen, aunque no llegaron a poseer un resultado estadísticamente significativo. En cuanto, al número de exacerbaciones, obstrucción intestinal y visitas a emergencia no se obtuvo los suficientes artículos para realizar metaanálisis. Conclusiones: CDI aumento estadísticamente significativo el tiempo hospitalización, readmisiones y mortalidad en los pacientes con EII.
Exploration of Increasing Students’ Critical Thinking in the Guided Discovery Learning Model on Light Waves
Authors: Zahra Syarifah , Suwarna Iwan Permana
Published: October 2023

This study explored increasing students' critical thinking in the guided discovery learning model on light waves at one of the high schools in South Tangerang City. The research design employed a quasi-experimental nonequivalent control group design with students from class XI IPA 3 as the experimental group and class XI IPA 5 as the control group. Data was collected using a pretest and posttest instrument comprising ten essay questions and was analyzed using the n-gain method. The N-gain analysis indicated a significant improvement in critical thinking skills, with a score of 0,60 for the experimental group and 0,25 for the control group. This study concludes that using the guided discovery learning model effectively enhances students' critical thinking skills in light waves at one of the high schools in South Tangerang City. The implications of this study indicate that implementing the guided discovery learning model in teaching light wave material is highly relevant for enhancing students' critical thinking abilities. This approach enables students to actively engage, analyze information, and solve problems, thereby fostering the development of their critical thinking skills. By acquiring critical thinking skills through guided discovery learning, students become better prepared to tackle complex challenges and support integrating critical thinking development into diverse subjects within the curriculum.
On Duality Principles and Related Convex Dual Formulations Suitable for Local Non-Convex Variational Optimization
Authors: Botelho Fabio
Published: October 2023

This article develops duality principles, a related convex dual formulation and a primal dual one suitable for the local optimization of non-convex primal formulations for a large class of models in physics and engineering. The results are based on standard tools of functional analysis, calculus of variations and duality theory. In particular, we develop applications to a Ginzburg-Landau type equation. We emphasize the novelty here is that the first dual variational formulation developed is convex for a primal formulation which is originally non-convex. Finally, we also highlight the second primal dual variational formulation presented has a large region of convexity around any critical point.
Effects of casein hydrolysate prepared with savinase on the quality of breads made by frozen dough
Authors: Bekiroglu Hatice , Ozulku Gorkem , Sagdic Osman
Published: October 2023

The effect of casein savinase hydrolysate (CSH) usage on frozen dough (1%, 1.5% and 2%, g/100 g flour) was investigated in terms of rheological, thermal, structural characteristics of wheat doughs and the textural and color properties of corresponding breads. Rheological measurement showed that CSH addition into dough led to a reduction of Gʹ and Gʹʹ values, but a similar trend wasn’t observed in frozen dough samples. The increase of protein band intensity was observed for control dough (CD) after frozen storage (-30 °C, 28 days) while there was no increases in band intensities of the doughs with CSH. The freezable water content of unfrozen doughs was decreased gradually with addition of CSH depended on concentration level. Frozen storage caused a notable reduction in α-helices structure of CD sample (p<0.05) while no significant variation was observed for the doughs containing CSH (p>0.05). Lowest specific volume reduction and hardness increment were observed for the breads containing 1.5% and 2% CSH. Frozen storage caused a significant reduction on b* value of bread crust (p<0.05) while no significant effect was observed for L* and a* value during frozen storage (p > 0.05). Overall, CSH incorporation into frozen dough can be an alternative that could reduce the quality deteriorations of frozen bread.
Correlation of Anthropometric Characteristics and Movement Behavior with Lower Extremity Muscle Power and Dynamic Knee Valgus in Adolescent Basketball Athletes
Authors: Stojiljkovic Aleksandar , Karagianni Eleni , Antoniou Varsamo , Pepera Garyfallia
Published: October 2023

Obesity in adolescents combined with the increased exposure time to electronic devices, enhances a sedentary lifestyle and is linked to the risk of developing musculoskeletal injuries. The aim of this study was to identify possible correlations between anthropometric characteristics, body composition, exposure time to electronic devices or screen time (ST) and physical activity (PA) level along with muscle power and dynamic knee valgus (DKV) in young athletes to avoid future injuries. A total of 125 adolescent basketball athletes (66.4% boys), 12 to 17 years old (13.94±1.58 years) participated. Anthropometric and demographic characteristics were recorded. PA level was assessed through IPAQ and ST through a single question from the HELENA study. The Counter Movement Jump test was used for the evaluation of the lower limb muscle power, while the Single Leg Drop Jump test was used for the evaluation of the dynamic knee valgus via two-dimensional (2D) kinematic analysis. The Pearson index showed a statistically significant positive correlation between muscle power and height (r=0.788, p<0.001), while the Spearman index showed a smaller but equally statistically significant positive correlation with BMI (rs =0.651, p<0.001) and age (rs=0.579, p<0.001). The ST (rs= -0.194, p=0.03) showed a weak negative correlation with DKV, while PA (r=0.85, p<0.001) showed a high, statistically significant, positive correlation. The anthropometric characteristics of adolescent athletes can determine their ability to produce muscle power. A high level of physical activity could play a key role in compensating and thus reducing the negative effects of high exposure to screen time on the dynamic knee valgus
Recent Progress in Mass Spectrometry-based Metabolomics in Major Depressive Disorder Research
Authors: Liu Mingxia , Ma Wen , He Yi , Sun Zuoli , Yang Jian
Published: October 2023

Major depressive disorder (MDD) is a serious mental illness with a heavy social burden, but its underlying molecular mechanisms remain unclear. Mass spectrometry (MS)-based metabolomics is providing new insights into the heterogeneous pathophysiology, diagnosis, treatment, and prognosis of MDD by revealing multi-parametric biomarker signatures at the metabolite level. In this comprehensive review, recent developments of MS-based metabolomics in MDD research are summarized from the perspective of analytical platforms (liquid chromatography-MS, gas chromatography-MS, supercritical fluid chromatography-MS, etc.), strategies (untargeted, targeted, and pseudotargeted metabolomics), key metabolite changes (monoamine neurotransmitters, amino acids, lipids, etc.), and antidepressant treatments (both western and traditional Chinese medicines). Depression sub-phenotypes, comorbid depression, and multi-omics approaches are also highlighted to stimulate further advances in MS-based metabolomics in the field of MDD research.
Effect of L. crispatus M247 Administration on Pregnancy Out-comes in Women Undergoing IVF: a Controlled, Retrospective, Observational and Open-label Study
Authors: Di Pierro Francesco , Sinatra Francesco , Cester Maddalena , Da Ros Lucia , Pistolato Mara , Da Parè Vania , Fabbro Laura , Maccari Daniela , Dotto Silvia , Sossai Sara , Fabozzi Gemma , Bertuccioli Alexander , Cazzaniga Massimiliano , Recchia Martino , Zerbinati Nicola , Guasti Luigina , Baffoni Andrea
Published: October 2023

Aim of our study was to retrospectively evaluate whether the oral administration of L. crispatus (M247) could increase pregnancy and live birth rates in women undergoing assisted reproductive technology procedures. Enrolled women (N=160) were divided into two groups: treated (N=80) or untreated (N=80) with the probiotic strain. The odds ratio for a treated woman to get a positive pregnancy test (PPT) was 1.56. In women aged 30–40 years, M247 increased the probability of a PPT in correlation with the progressive rise in BMI, reaching 47% (35% in controls) with a BMI of 35 (odds ratio: 2.00). The CAID statistics showed that in a woman of the blastocyst subgroup, below 43 years, with a BMI over 18.6, treatment with M247 increased the chance of a PPT from 28.4% to 44.5% (odds ratio: 2.08; p<0.05). Considering live births, rate of probiotic group was 12.5% versus 7.5% (odds ratio=1.76). Considering only the blastocyst subgroup, the treatment increased the number of live births by 200% (odds ratio: 3.64; p=0.05). As confirmed also by statistical indices like NNT, NNH and LHH, the use of M247 demonstrated a risk-benefit ratio to the full advantage of the benefits.
The Effect of a Preconception Maternal Mentoring Program on Fetal Growth among Indonesian Women: Results of a Cluster Randomized Controlled Trial
Authors: Hadi Hamam , Nurunniyah Siti , Gittelsohn Joel , Alfiana Ratih Devi , Fatimatasari Fatimatasari , Lewis Emma Clare , Nurdiati Detty Siti
Published: October 2023

The prevalence of stunting in young Indonesian children is the highest among Southeast Asian Nations, and is associated with poor growth during the prenatal and early postnatal periods. A maternal mentoring program was developed for Indonesian women to improve birth outcomes. A cluster randomized control trial (CRCT) was conducted in three sub-districts of the Special Re-gion of Yogyakarta, Indonesia. A total of 384 eligible participants were randomly allocated to either intervention (received maternal mentoring program and standard care; n=189) or control (received standard care only; n=195). The maternal mentoring program provided preconception health education; health monitoring; and text message reminders for preconception women. Fetal growth was measured between gestational weeks 27-30 using estimated fetal weight generated from ultrasonographic measurement. Birth weight was measured within 24 hours of birth. A structured questionnaire captured women’s’ demographics, pregnancy readiness, and body mass index (BMI. After adjustment, fetal weight was 14% (95% CI: 5.1-23.0) higher in the intervention group than in the control group, and the average weight-for-length Z-score at birth was 0.16 (95% CI: 004-0.30) higher in the intervention group than in the control group. A maternal mentoring program was associated with improved fetal growth and birth weight in this population.
Child Safety Seat‒Use Behavior Among Parents of Newborns: A Trans-Theoretical Model‒Guided Intervention in Shantou, China, 2021
Authors: Peng Yixiang , Wu Feng , Yang Jingzhen , Li Liping
Published: October 2023

We evaluated the effect of a trans-theoretical model–based intervention on child safety seat (CSS)‒use behaviors among parents of newborns in Shantou, China. Parents’ knowledge, attitude, and self-efficacy scores on CSS use were significantly higher at one, three, and six months after the intervention compared with scores at baseline. Parents’ stage of CSS use also advanced as the intervention progressed. The trans-theoretical model‒based intervention and evidence generated from this study may provide guidance for future individualized interventions to improve CSS use. (Am J Public Health. 2023;113(12):1271–1275. https://doi.org/10.2105/AJPH.2023.307415 )
La metáfora conceptual del discurso politico en alemán y español
Authors: Corbacho Sánchez Alfonso
Published: October 2023

El presente estudio se centra en la metáfora cognitiva circunscrita al lenguaje político del alemán y el español, sometiendo a examen un corpus léxico procedente de varios métodos de enseñanza del alemán como lengua extranjera. Para ello, las muestras seleccionadas se presentan de forma sistemática de acuerdo con la taxonomía habitual de la metáfora cognitiva junto a sus formas equivalentes en español. Los resultados revelan, en primer lugar, que en el lenguaje político se aprecia un número importante de metáforas conceptuales; y además ponen de manifiesto las convergencias y las divergencias entre estas dos lenguas que sirven de vehículo a dos culturas diferentes. Este trabajo también reafirma la utilidad del análisis contrastivo para la traducción y para la enseñanza y el aprendizaje del alemán y el español como lenguas extranjeras, y desde luego contribuye a comprender el grado en que se diferencian estas dos lenguas.
RESEÑA: CASTELLANO MARTÍNEZ, José María, Fundamentos nocionales y traductológicos para la traducción de textos turísticos promocionales. Granada: Comares, 154 páginas. ISBN:
Authors: Guijarro Moya Rafael
Published: October 2023

Reseña elaborada por Rafael Guijarro Moya
MARTINO ALBA, Pilar; VEGA CERNUDA, Miguel Ángel (coords.), La Gran Guerra en la literatura y las artes. Análisis de testimonios. OMMPRESS TRADUCCIÓN, 2018. ISBN: 978-84-17387-25-9.
Authors: Holzer Peter J.
Published: October 2023

Reseña de la publicación de referencia.
Artificial Intelligence (AI) and Machine Learning (ML) Enabler for Asset Data Sustainability and Governance
Authors: Jadhav Satyaprasad
Published: October 2023

The Asset Information of an operating plant are increasingly recognized as a vital part of the facility and have more recently been characterized as "The Plant Information Asset", reflecting the value in the data that sits alongside the physical asset or facility. The generation of the Plant Information Asset is achieved in parallel during each phase of the Contractor's project cycle, coordinated to record the precise details of the As-Built installation.

The handover of information consists of the documents and data created during the Engineering process and relationships between documents to Tags and documents to vendor, etc.

Industry studies estimate that as much as 70% of industry capital and operating expense is invested into the processing, maintenance, and handling of plant engineering information. Without proper information management practices in place the value and usefulness of asset-related material can quickly degrade to a point where its actual use in Operations and Maintenance is jeopardized. Data delivered to the Owner/Operator (O/O) from a Contractors (external entity) not adhering to certain specifications always increases data governance costs and has a negative impact on asset commissioning and overall response effort for Asset Information Sustainability and Governance.

Artificial Intelligence (AI) And Machine Learning (ML) can now offer significant capabilities to optimize the quality check process and ensure quality data and Information handover from contractor to Owner Operator. Specifically, the Optical Character Recognition and neuro-linguistic programming methods are used to perform quality check inside the documents itself and check for tag patterns, as-built stamp, tag to document relationships, revisions, metadata etc. This study focuses on how Artificial Intelligence (AI) And Machine Learning (ML) can be enabler to sustain Asset Data and Information Management during Engineering, operation, maintenance, and HSE.
The specific regulatory networks between different variants in papillary thyroid carcinoma: A transcriptomics study
Authors: Xie Li-Jun , Yang Shi-ci , He Rui , Wang Tingting , Yang Zhi-yu , Song Shu , Feng Jiao-jiao , Zhu Gao-Hong
Published: October 2023

Objective: To analyze the molecular pathogenesis or characteristics based on transcriptomics techniques to diagnose and treat papillary thyroid carcinoma (PTC) caused by mutations.
Methods: We conducted transcriptome sequencing to identify differentially expressed genes (DEGs) in PTC-derived cell lines TPC-1, BCPAP, IHH4, and CVPTC. Additionally, gene expression profiling was performed using microarray in GEO database to screen out representative dataset chips and analyze DEGs of PTC tissues in the clinic. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses on DEGs were performed using DAVID database. We established the protein-protein interaction (PPI) network using STRING database and built the transcription factor (TF) regulation network based on module analysis for identifying master regulators along with gene modules.
Results: We identified 4,353 and 3,250 DEGs among cell line (CVPTC/BCPAP/IHH4 or TPC-1/BCPAP/IHH4) and normal thyroid cells independently. After screening GSE27155 dataset, we identified 1,075 DEGs among PTC tissue samples (classic/RET_PTC mutation/high cellular variant) and normal tissue samples. The DEGs in the three groups were enriched in different pathways like cell proliferation, signal dysregulation, immune dysregulation, angiogenesis and cancer pathways. By constructing the PPI network and performing the module analysis, we determined top 10 key genes in the three groups separately. The results showed that 22 and 61 important TFs were significantly enriched in PPI of the two cell groups respectively and only one important TF was significantly enriched in PPI of PTC tissues.
Conclusion: We identified the transcriptomic features of PTC cell lines and clinical PTC tissues associated with invasive behavior.
Obstetric and neonatal outcomes of extremely obese pregnant women after late preterm gestation
Authors: Choodinatha Harshitha Kallubhavi , Lee Jin , Lee Min Jung , Choi Bo Young , Kim Hyeon Ji , Park Jee Yoon
Published: October 2023

Background
Maternal obesity characterized by high body mass index(BMI) is linked to adverse maternal and neonatal outcomes. However, insufficient data exist about the impact of extreme obesity on pregnancy within the Asian population.
Objective
To determine the obstetric and neonatal outcomes of pregnant women with extreme obesity at birth after late preterm gestation.
Methods
A retrospective study on extremely obese pregnant women with BMI ≥ 40kg/m2, obesity class III, excluding fetal death in utero and preterm births before 34 weeks of gestation. Obstetric and neonatal outcomes were reviewed.
Results
The study involved 94 extremely obese pregnant women (median BMI at delivery: 42.4 kg/m2). In relation to the prepregnancy BMI obesity class II category, higher chronic hypertension rates were found in allegedly extremely obese women compared to those with lower pre-pregnancy BMI (34% vs. 10%, p = 0.012), while the tocolytics use for preterm labour was higher in the BMI < 35.0 kg/m2 group (26% vs. 5%, p = 0.007). Adverse neonatal outcomes, including neonatal intensive care unit admission, use of respiratory support and jaundice were higher in the pre-pregnancy BMI < 35.0 kg/m2 group than the BMI ≥ 35.0 kg/m2 group (all p-values < 0.05). The use of neonatal support increased with lower pre-pregnancy BMI and with higher weight gain during pregnancy.
Conclusions
In extremely obese women(class III) who delivered during late preterm gestation, the use of tocolytics for preterm labour and the incidence of adverse neonatal respiratory outcomes appeared to be higher among those who were less obese before pregnancy than among those who were already extremely obese.
Quality of Life in Women with Breast Cancer Undergoing Neoadjuvant Chemotherapy: Comparison Between PICC and PICC-port
Authors: Pinelli Fulvio , Barbani Francesco , Defilippo Barbara , Fundarò Angela , Nella Alessandra , Selmi Valentina , Romagnoli Stefano , Villa Gianluca
Published: October 2023

Background
Peripherally inserted central catheters (PICCs) and new type of arm-port, the PICC-port, are currently used for neoadjuvant chemotherapy treatment in patients with breast cancer. We aimed to compare Quality of Life (QoL) of patients receiving one of these two devices investigating overall satisfaction, psychological impact as well as the impact on professional, social and sport activities, and local discomfort.
Methods
We did a prospective observational before-after study of PICCs versus PICC-ports. Adult (aged ≥18 years) female with breast cancer candidate to neoadjuvant chemotherapy were included. The primary outcome was QoL according to the Quality-of-Life Assessment Venous Device Catheters (QLAVD) questionnaire assessed 12 months after device implantation.
Results
Between May 2019 and November 2020, of 278 individuals screened for eligibility, 210 were enrolled. PICC-ports were preferred over PICCs with a QLAVD score of 29 [25; 32] vs 31 [26; 36.5] (p = 0.014). Specifically, most QLAVD constructs related to psychological impact, social aspects, and discomfort were in favor of PICC-ports vs PICC, especially in women under the age of 60. Overall, pain scores at insertion and during therapy administration were not significantly different between the two groups, as well as infection, secondary malpositioning, thrombosis or obstruction of the device.
Conclusions
In women with breast cancer undergoing neoadjuvant chemotherapy, PICC-ports were overall better accepted than PICCs in terms of QoL, especially in those who were younger. Device-related complications were similar.
Oncoproteins E6/E7 of the Human Papillomavirus Types 16 & 18 Cooperate in Modulating Oncogenes and Tumor Suppressor Proteins in Colorectal Cancer
Authors: Fernandes Queenie , Therachiyil Lubna , Younis Shahd M , Dermime Said , Moustafa Ala-Eddin Al
Published: October 2023

Background This study presents a novel analysis of the oncogenes and tumor suppressor proteins directly modulated by E6/E7 of high-risk HPV types 16 and 18, in two mutational cell models of colorectal cancer (CRC).Methods Two distinct mutational cell models of colorectal cancer, HCT 116 (KRAS mutant) & HT-29 (TP53 mutant) were transfected with E6/E7 of HPV16 and HPV18, individually and in combination (co-transfection), via lipofection. Further, we utilized a liquid chromatography mass spectrometry (LC-MS/MS) approach to analyze and compare the proteomes of KRAS mutant HCT 116 and TP53 mutant HT-29 cell models of CRC, expressing E6/E7 of HPVs type 16 and 18, both, individually and in combination.Results We generated six stably transfected cell lines, namely, HCT 116 HPV16+, HCT 116 HPV18+, HCT 116 HPV16+/18+, HT-29 HPV16+, HT-29 HPV18+, HT-29 HPV16+/18+. Our proteomics data revealed a significantly higher, HPV-induced, modulation of oncogenes and tumor suppressor proteins in the TP53 mutant model of CRC, as compared to the KRAS mutant cell model (p ≤ 0.01). Less than 1% of the genes were found to be commonly modulated by HPV, between the two models of CRC. In addition, we report that HT-29 cells, expressing E6/E7 of both HPVs 16 and 18, significantly reduced the downregulation of oncogenes as compared to cells expressing E6/E7 of either HPV16 or HPV18 individually (p-value is ≤ 0.00001).Conclusion Our data imply that coinfections with the two most frequently observed high-risk HPV types worldwide, leads to the sustenance of a pro-oncogenic environment in CRC; that is suggestive of the cooperative role of the viruses in inflicting cancer. Further, HPV modulates different sets of oncogenes/tumor suppressor proteins in CRC models of varying mutational backgrounds, thus highlighting the importance of personalized therapies for diseases with mutational heterogeneities.
Identification of autophagy related genes predicting response of trastuzumab resistance in breast cancer patients
Authors: liu yaping , hao xinlei , li yuntao , hong yang
Published: October 2023

Background The emergence of trastuzumab resistance is the barrier to effective clinical outcomes for HER2 + Breast Cancer (BC). However, the relationship between the expression of autophagy related genes in HER2 + BC and their correlation with prognosis remains unclear. This study aims to identify the potential predictive markers through bioinformatics analysis and experiment validation. Methods Gene expression profile dataset GSE29431 was obtained from GEO database. Autophagy related differentially expressed genes (ARGs) of HER2 + BC were identified by R software. Multiple bioinformatics analyses were conducted to identify functional hub genes. The prognostic significance of these hub genes was validated, and the correlations between ARGs, clinicopathological parameters, and patient prognosis were analyzed using The Cancer Genome Atlas (TCGA) cohort. Results A total of 73 ARGs were identified between 41 HER2 + BC patients and 12 normal samples. Functional analysis, including Gene Ontology (GO), protein-protein interaction (PPI), and Kyoto Encyclopedia of Genes and Genomes (KEGG), revealed important functional genes related to macroautophagy, late endosome formation, and ubiquitin-like metabolism, which were identified as autophagy-related hub genes. Additionally, PPP1R15A, VAMP7, PTK6, CASP3 demonstrated strong predictive power in the TCGA cohort through prognostic correlation analysis (p < 0.05). The results of qRT-PCR and immunohistochemistry demonstrated increased expression of VAMP7 and PTK6 in BC patients with trastuzumab resistance, further confirming their prognostic significance in a trastuzumab adjuvant treatment cohort using our clinical data. Conclusions Patients with high expression of VAMP7 and PTK6 exhibit poor efficacy and prognosis in HER2 + BC, particularly in the context of trastuzumab neoadjuvant chemotherapy.
Substituting maize with blends of Solanum tuberosum chips and Ensete ventricosum corm on egg production and quality in Bovans Brown
Authors: Kecheroo Yisehakk Kebede , Weji Gossaye
Published: October 2023

The experiment was conducted in the Sodo district of the Gurage zone, in the central highlands of Ethiopia, to ascertain the effects of substituting enset (Ensete ventricosum) corm and potato (Solanum tuberosum) chips for corn on the quantity and quality of eggs laid by Bovans brown layers. A total of 132 Bovans brown layers aged 24 weeks were used in the investigation. All 132 of the Bovans brown layers in the study were 24 weeks old. In place of maize grain, the layers were fed feed that contained 0% (T1), 15% (T2), 30% (T3), and 45% (T4) of a potato chip and enset corm mixture. The trial was totally randomized and comprised four treatments. With 11 birds apiece, each treatment was carried out three times. A 12-week experiment was conducted. Hens were weighed at the start and end of the trial. Dry matter intake, hen-day egg production (HDEP), egg weight, and egg mass were all tracked daily. Every 15 days, four eggs per replicate were evaluated for four different egg quality metrics (egg shell weight and thickness, albumen weight and height, Haugh unit and yolk weight, and color). Mix of potato chips and enset corms with 3568 kcal/kg DM, 4.28% CP, 0.17% ether extract, and 2.40% crude fiber. The average daily increases of T1 (2.08g), T4 (1.99g), and T3 (1.85g) were comparable and significantly larger (P 0.05) than T3 (1.28g). The egg mass and feed conversion ratios among treatments were statistically comparable (P < 0.05). T1's (81.08%) HDEP was equivalent to and significantly (P 0.05) higher than T3 (84.07%), T4 (83.61%), and T2 (83.34%). Compared to T1 and T3, T4 showed a higher eggshell weight (P 0.05). As a result, enset corm and potato chips can be combined to substitute maize in layer rations when it is scarce in enset and potato-growing regions.
Effects of Sucrose and 6-Benzylaminopurine Concentrations on Shoot Regeneration and Vitrification in Aronia melanocarpa: Insights for Plant Tissue Culture Systems
Authors: Bayhan Nida , Yucesan Buhara
Published: October 2023

This study focuses on investigating the effects of sucrose and 6-Benzylaminopurine (BAP) concentrations on shoot regeneration and the development of vitrification in Aronia melanocarpa. Initially, the presence of BAP, in combination with varying sucrose concentrations, leads to a substantial increase in shoot number. The highest shoot formation is observed in the MS medium containing 3% sucrose and 5.0 mg/L BAP. Furthermore, sucrose concentration plays a crucial role in shoot growth, with higher concentrations promoting more extensive shoot development. However, when sucrose levels were at 3% combined with higher BAP concentrations (1.0 or 2.5 mg/L), an increased incidence of vitrification was observed over time. Interestingly, lower sucrose concentrations (1% or 2%) combined with 0.5 mg/L or 2.5 mg/L BAP initially delayed vitrification but eventually led to its occurrence. Microscopic analysis of leaf samples with varying levels of vitrification indicates significant differences in stoma characteristics, such as density and size, further confirming the detrimental impact of vitrification on cellular structures and physiological processes. The recovery of vitrified plants was evaluated using different growth media combinations. The absence of BAP in the medium led to higher recovery percentages without necrosis, while the addition of 0.5 mg/L BAP promoted shoot growth but potentially inhibited root development. During acclimatization, vitrified plants exhibited stunted shoot growth, shorter and narrower leaves, reduced root numbers and lengths, and decreased survival rates, particularly under lower humidity conditions. The cultivation period required for the recovery of the crop was determined to be six weeks under controlled greenhouse conditions.
Cerebral filariasis infection with Litomosoides in Molossus barnesi (Chiroptera: Molossidae) in the brazilian eastern amazon, with comments on Molossinema Wimsatti Georgi, Georgi, Jiang and Fronguillo, 1987
Authors: Conga David , de Araújo Camille , de Souza Natália , Corrêa Jean , Santos Josye , Figueiredo Edigleise , Bernal Marcella , Andrade Sara , Silva Neuder da , Pereira Washington , Gardner Scott , Notarnicola Juliana
Published: October 2023

During the health evaluation of 30 specimens of bats captured in periurban forest areas in the city of Belém–Pará, Brazil, a case of cerebral filariasis was observed. In the histopathological examination, adult filariae were found in the meningeal membranes in the brain tissue of Molossus barnesi (Molossidae) and classified morphologically as Litomosoides by the shape of the spicules –left spicule with a handle longer than the blade; right spicule curved, with a sclerotized heel supporting a dorsal notch; the area rugosa constituted by a ventral band of small longitudinal crests; tail rounded in males; long esophagus with a slightly glandular distal portion, and a muscular bent vagina. All the specimens lack a stoma (buccal capsule). We compared our filarioids with the description and of specimens of Molossinema wimsatti. Morphological characters from M. wimsatti resemble the genus Litomosoides. Thus, we believe that M. wimsatti is a synonym of L. molossi Esslinger, 1973, and filarioid specimens from material reported by Lichtenfels et al. (1981) and from de Souto et al. (2021) most probably correspond to Litomosoides. We suggest that the reduction of the buccal capsule could be attributable to the ectopic location. No evidence of tissue responses by the host were observed. This is the first record of Litomosoides infecting brain tissue of Molossus barnesi from Brazil, representing a new host record. More specimens of bats should be examined in order to find filarioids in the brain and verify its taxonomic position using molecular techniques.
Comparison of breathing pattern and diaphragmatic motion in patients with unilateral cervical radiculopathy and asymptomatic group
Authors: Yousefiyan Raziyeh , Yoosefinejad Amin Kordi , Jalli Reza , Rezaei Iman
Published: October 2023

Background: Recently, there has been growing interest in the research and management of respiratory dysfunctions in patients with neck pain. Although patients with cervical radiculopathy (CR) are predominantly managed as neuromusculoskeletal patients, currently, there is evidence that they also have pulmonary dysfunction. Although many studies have investigated respiratory dysfunction in patients with chronic neck pain, this is the first study to examine the respiratory motion of the diaphragm in CR. The purpose of this study was to compare the breathing pattern and diaphragmatic excursion in patients with unilateral CR with those in an asymptomatic group.
Methods: Twenty-five patients with unilateral CR and 25 asymptomatic individuals between 30 and 55 years old participated in this study. Diaphragmatic motion, breathing pattern, active cervical range of motion (ACROM) and kinesiophobia were investigated in both groups by using fluoroscopy, manual assessment of respiratory motion (MARM), cervical range of motion device, and Tampa Scale of Kinesiophobia (TSK) questionnaire. Statistical significance was set at 0.05.
Results: No statistically significant differences were found between the two groups with regard to sex, age and body mass index (BMI). The mean excursion of the hemi diaphragm on the involved side (the side of CR) was significantly lower than that on the uninvolved side in patients with unilateral CR with a large effect size. The excursion of the involved hemi diaphragm in patients was reduced compared to the matched hemi diaphragm in the control group. There was no significant difference between the hemi diaphragm excursion in the control group. The results of the MARM variables showed that the volume of breathing and the percentage rib cage motion in normal and deep breathing were significantly different between the two groups, but there was no significant difference in the balance of breathing between the two groups. Additionally, the CROM was reduced in these patients in comparison to the control group, and it was less on the involved side than on the uninvolved side.
Conclusion: The results of this study revealed a dysfunctional breathing pattern in normal and deep breathing and a unilateral reduction in diaphragmatic excursion on the side of radiculopathy in patients with unilateral CR compared to the control group.
Hyper-methionine Feeding Influence diabetes nephropathy (DN) by regulating the methylation status of kidney in STZ-induced diabetes rats
Authors: Jiang Yongwei , Deng GuoXiong , Liu Chengyin , Tang Han , Zheng Jing , Kong Xiaomu , Zhao Meimei , Liu Yi , Gao Peng , Li Tianbao , Zhao Hailing , Cao Yongtong , Li Ping , Ma Liang
Published: October 2023

This study aimed to investigate the impact of hyper-methionine (HM) feeding on diabetic nephropathy (DN) pathogenesis by examining and analyzing differential methylation profiles. Eight-week-old male Wistar rats (250–300g) were randomly divided into four groups: control group (healthy, n = 8), streptozocin (STZ) induced DN rats (STZ group, n = 8), STZ-induced DN rats fed with HM (STZ + HM group, n = 8), and the Tangshen Formula (TSF) treatment for STZ + HM rats (TSF group, n = 8). Blood glucose and other metabolic indicators were monitored before treatment and at four-week intervals until 12 weeks. Total DNA was extracted from the aforementioned groups, and DNA methylation profiles were analyzed using reduced representative bisulfite sequencing (RRBS).The STZ group and STZ + HM group exhibited increased levels of blood glucose and urinary albumin/creatinine compared to control group. STZ + HM group showed significantly higher levels of urinary albumin/creatinine (411.90 ± 88.86 mg/g) than the STZ group (238.41 ± 62.52 mg/g). A total of 71,957 differentially methylated regions (DMRs) were identified, with 48,950 (68.02%) being hyper-methylated and 23,007 (31.98%) being hypo-methylated. In-depth analysis of DNA methylation profiles revealed 19 genes, out of 797 methylated genes associated with DN. HM exacerbates DN through altered methylation patterns at specific CpG sites. TSF group demonstrated significant reductions in glucose and urinary albumin/creatinine levels compared to the STZ + HM group. Furthermore, TSF shows potential as an effective treatment for DN by restoring aberrant methylation levels. The identification of specific genes associated with DN provides valuable insights into the underlying mechanisms of DN pathogenesis and offers potential therapeutic targets for further investigation.
Application of rigid bronchoscopy with endoscopic bronchial ultrasound-guided transbronchial node biopsy in pulmonary sarcoidosis
Authors: wan nansheng , meng han , wang zhongli , shi yu , Feng Jing
Published: October 2023

Background
Pathological examination of diseased lymph nodes is crucial for diagnosing pulmonary sarcoidosis, and obtaining quality histological specimens is important for improving the diagnosis rate. This study assessed the diagnostic rate and safety differences associated with performing endobronchial ultrasound-guided transbronchial node biopsy plus rapid on-site evaluation versus traditional endobronchial ultrasound-guided transbronchial needle aspiration plus rapid on-site evaluation for pulmonary sarcoidosis.
Methods
This retrospective study included data from 110 patients diagnosed with pulmonary sarcoidosis after undergoing rigid bronchoscopy combined with endobronchial ultrasound to obtain hilar or mediastinal lymph node specimens between March 2016 and May 2022. A rapid on-site evaluation was used to evaluate all intraoperative samples.
Results
Diagnostic rates of pulmonary sarcoidosis via endobronchial ultrasound-guided transbronchial node biopsy and endobronchial ultrasound-guided transbronchial needle aspiration were 94.0% (63/64) and 72.1% (31/43; relative risk = 1.30, 95% confidence interval: 1.07–1.59; P = 0.002), respectively. Regarding intraoperative safety, four (9%) patients in the endobronchial ultrasound-guided transbronchial needle aspiration group and five (7%) patients in the endobronchial ultrasound-guided transbronchial node biopsy group had intraoperative airway bleeding without intervention (relative risk = 1.02, 95% confidence interval: 0.91–1.15; P = 0.74). There were no other between-group differences in postoperative adverse effects.
Conclusions
Compared with traditional endobronchial ultrasound-guided transbronchial needle aspiration combined with rapid on-site evaluation, endobronchial ultrasound-guided transbronchial node biopsy combined with rapid on-site evaluation is of increased diagnostic value when assessing mediastinal or hilar lymphadenopathy suspected to be pulmonary sarcoidosis. Further, no significant differences in intra- or postoperative complications and adverse reactions were observed.
Pathogenic variants in human DNA damage repair genes mostly arose in recent human history
Authors: Zhao Bojin , Li Jiaheng , Sinha Siddharth , Qin Zixin , Kou Si Hoi , Xiao Fengxia , Lei Huijun , Chen Tianhui , Cao Wenming , Ding Xiaofan , Wang San Ming
Published: October 2023

Background
Genome stability is maintained by the DNA damage repair (DDR) system composed of multiple DNA repair pathways of hundreds of DDR genes. Germline pathogenic variation (PV) damages function of the affected DDR genes, leading to genome instability and high risk of cancer. Knowing evolutionary origin of human DDR PV is essential to understand the etiology of human cancer. However, answer to the issue remains largely elusive. In the study, we analyzed evolutionary origin for the PVs in human DDR genes.
Methods
We collected 169 DDR genes by referencing various databases, analyzing cross-species genomic data using the phyloFit program of the PHAST package, with visualization using GraphPad Prism software and the ggplot module. Ancient and modern human DDR PV detection and comparison using tools such as SAMtools and variant annotation databases. Evolution selection studies across 20 vertebrates used CodeML in PAML for phylogenetic analysis, and the MEGA were used for sequence alignment. We created an open-access database using a LAMP stack and performed statistical analyses using Pearson correlation and Kruskal-Wallis tests.
Results
Through phylogenic analysis, we ruled out cross-species conservation as the origin; using archeological approach, we identified rich DDR PVs shared between modern and ancient humans, which were mostly dated within the last 10,000 years. We observed similar pattern of quantitative PV distribution between modern and ancient humans. We also detected a group of ATM, BRCA2 and CHEK2 PVs shared between human and Neanderthals.
Conclusions
Our study reveals that human DDR pathogenic variation mostly arose in recent human history, and human high cancer risk caused by DDR PVs can be a by-product of human evolution.
Knowledge, attitudes and practices of critical care unit personnel regarding pediatric palliative care: A cross-sectional study
Authors: Lu Hua , Jin Linfei
Published: October 2023

Background
Few studies have evaluated the perceptions of healthcare providers in China regarding pediatric palliative care, particularly in critical care units (PICUs), where many children receive palliative care. To evaluate the knowledge, attitudes and practices of PICU personnel in China regarding pediatric palliative care.
Methods
This cross-sectional study was conducted in five cities in China (Shanghai, Suzhou, Chongqing, Chengdu and Yunnan) between November 2022 and December 2022.
Results
The analysis included 204 participants (122 females), with 158 nurses and 46 physicians. The average knowledge, attitude and practice scores were 9.75 ± 2.90 points (possible range, 0–13 points), 38.30 ± 3.80 points (possible range, 12–60 points) and 35.48 ± 5.72 points (possible range, 9–45 points), respectively. Knowledge score was higher for physicians than for nurses (P < 0.001) and for personnel with previous training in pediatric palliative care (P = 0.017), with previous theory-based training (P = 0.008), and who worked in a department where pediatric palliative care was available (P = 0.015). According to structural equation modelling knowledge had a direct positive effect on attitude (β = 0.221 (0.088–0.346), p = 0.003), but influence of knowledge or attitude on practice was insignificant.
Conclusions
There is room for improvement in the knowledge, attitudes and practices of PICU personnel in China regarding pediatric palliative care. The findings of this study may facilitate the design and implementation of targeted education/training programs to better inform physicians and nurses in China about pediatric palliative care.
Current and future predicted distribution range of Indian Flying Fox Pteropus medius Temmink, 1825 in Nepal
Authors: Thapa Sanjan , Dahal Dibya Raj , Baral Suraj , Pandey Kishor , Thapa Tej Bahadur , Racey Paul A. , Yu Wenhua , Wu Yi
Published: October 2023

Pteropus medius is exclusively distributed in South Asia. Among four species of fruit bats occurring in Nepal, the Indian Flying Fox is the largest of all. Their known roosts are distributed within an elevation range of 75 to 1,322 m asl throughout east west in the Tarai, Chure and Inner-Tarai region and in central Middle Mountains of the country. The species is known to pollinate and disperse seeds of more than 26 families of flowering plants. Most of the known roosts lie within and in the close vicinity of human settlements and farmlands. Recently, impacts of global warming on roost and foraging habitat of fruit bats including flying foxes have been evidenced in different parts of the globe, however, it is still unknown in Nepal. In addition, most of the known roosting colonies of P. medius are outside the protected areas and are vulnerable due to greater threats. Based upon 44 roosts occurrence coordinates of P. medius and six low-correlated bio-climatic variables (r > 0.75), we built habitat suitability models using Maximum Entropy. The current potential distribution of the species covers an area of 38,688 km2 (About 26% of the country’s total area). Minimum Temperature of Coldest Month (bio 6), Precipitation of Coldest Quarter (bio 19) and Mean Diurnal Range (bio 2) are most influencing predictors. In the future, its overall distribution range in overall will slightly contract, although it will expand at the lower elevations in Tarai, Chure and Inner-Tarai regions. The future distribution is not expected to shift towards northern latitude and higher elevations.