bluuebunny/crossref_metadata_2025 huggingface dataset:
NEDD9 Restrains dsDNA Damage Response during Non-Small Cell Lung Cancer (NSCLC) Progression
Authors: Tikhomirova Mariya , Topchu Iuliia , Mazitova Aleksandra , Barmin Vitaly , Ratner Ekaterina , Sabirov Alexey , Abramova Zinaida , Deneka Alexander Y.
Published: May 2022

DNA damaging modalities are the backbone of treatments for non-small cell lung cancer (NSCLC). Alterations in DNA damage response (DDR) in tumor cells commonly contribute to emerging resistance to platinating agents, other targeted therapies, and radiation. The goal of this study is to identify the previously unreported role of NEDD9 scaffolding protein in controlling DDR processes and sensitivity to DNA damaging therapies. Using a siRNA-mediated approach to deplete NEDD9 in a group of human and murine KRAS/TP53-mutant NSCLC cell lines, coupled with a set of cell viability and clonogenic assays, flow cytometry analysis, and Western blotting, we evaluated the effects of NEDD9 silencing on cellular proliferation, DDR and epithelial-to-mesenchymal transition (EMT) signaling, cell cycle, and sensitivity to cisplatin and UV irradiation. Using publicly available NSCLC datasets (TCGA) and an independent cohort of primary NSCLC tumors, subsequent in silico and immunohistochemical (IHC) analyses were performed to assess relevant changes in NEDD9 RNA and protein expression across different stages of NSCLC. The results of our study demonstrate that NEDD9 depletion is associated with the increased tumorigenic capacity of NSCLC cells. These phenotypes were accompanied by significantly upregulated ATM-CHK2 signaling, shifting towards a more mesenchymal phenotype in NEDD9 depleted cells and elevated sensitivity to UV-irradiation. IHC analyses revealed an association between reduced NEDD9 protein expression and a decrease in overall (OS) and progression-free survival (PFS) of the NSCLC patients. These data, for the first time, identified NEDD9 as a negative regulator of ATM kinase activity and related DDR signaling in numerous KRAS/TP53 mutated NSCLC, with its effects on the regulation of DDR-dependent EMT signaling, sensitivity to DNA damaging modalities in tumor cells, and the survival of the patients.
Bispecific BCMA-CD3 Antibodies Block Multiple Myeloma Tumor Growth
Authors: Wu Lijun , Huang Yanwei , Sienkiewicz John , Sun Jinying , Guiang Liselle , Li Feng , Yang Liming , Golubovskaya Vita
Published: May 2022

BCMA antigen is overexpressed in multiple myeloma cells and has been shown to be a promising target for novel cellular and antibody therapeutics. The humanized BCMA (clone 4C8A) antibody that effectively targeted multiple myeloma in a CAR (chimeric antigen receptor) format was used for designing several formats of bispecific BCMA-CD3 antibodies. Several different designs of univalent and bivalent humanized BCMA-CD3 CrossMAB and BCMA-FAB-CD3 ScFv-Fc antibodies were tested for binding with BCMA-positive cells and T cells and for killing by real time cytotoxic activity and IFN-gamma secretion with CHO-BCMA target cells and with multiple myeloma MM1S and H929 cell lines. All BCMA-CD3 antibodies demonstrated specific binding by FACS to CHO-BCMA, multiple myeloma cells, and to T cells with affinity Kd in the nM range. All antibodies with T cells specifically killed CHO-BCMA and multiple myeloma cells in a dose-dependent manner. The BCMA-CD3 antibodies with T cells secreted IFN-gamma with EC50 in the nM range. In addition, three BCMA bispecific antibodies had high in vivo efficacy using an MM1S xenograft NSG mouse model. The data demonstrate the high efficacy of novel hBCMA-CD3 antibodies with multiple myeloma cells and provide a basis for future pre-clinical and clinical development.
diTFPP, a Phenoxyphenol, Sensitizes Hepatocellular Carcinoma Cells to C2-Ceramide-Induced Autophagic Stress by Increasing Oxidative Stress and ER Stress Accompanied by LAMP2 Hypoglycosylation
Authors: Chiu Chien-Chih , Chen Yen-Chun , Bow Yung-Ding , Chen Jeff Yi-Fu , Liu Wangta , Huang Jau-Ling , Shu En-De , Teng Yen-Ni , Wu Chang-Yi , Chang Wen-Tsan
Published: May 2022

Hepatocellular carcinoma (HCC), the most common type of liver cancer, is the leading cause of cancer-related mortality worldwide. Chemotherapy is the major treatment modality for advanced or unresectable HCC; unfortunately, chemoresistance results in a poor prognosis for HCC patients. Exogenous ceramide, a sphingolipid, has been well documented to exert anticancer effects. However, recent reports suggest that sphingolipid metabolism in ceramide-resistant cancer cells favors the conversion of exogenous ceramides to prosurvival sphingolipids, conferring ceramide resistance to cancer cells. However, the mechanism underlying ceramide resistance remains unclear. We previously demonstrated that diTFPP, a novel phenoxyphenol compound, enhances the anti-HCC effect of C2-ceramide. Here, we further clarified that treatment with C2-ceramide alone increases the protein level of CERS2, which modulates sphingolipid metabolism to favor the conversion of C2-ceramide to prosurvival sphingolipids in HCC cells, thus activating the unfolded protein response (UPR), which further initiates autophagy and the reversible senescence-like phenotype (SLP), ultimately contributing to C2-ceramide resistance in these cells. However, cotreatment with diTFPP and ceramide downregulated the protein level of CERS2 and increased oxidative and endoplasmic reticulum (ER) stress. Furthermore, insufficient LAMP2 glycosylation induced by diTFPP/ceramide cotreatment may cause the failure of autophagosome–lysosome fusion, eventually lowering the threshold for triggering cell death in response to C2-ceramide. Our study may shed light on the mechanism of ceramide resistance and help in the development of adjuvants for ceramide-based cancer therapeutics.
Selective Deoxygenation of Sludge Palm Oil into Diesel Range Fuel over Mn-Mo Supported on Activated Carbon Catalyst
Authors: Alsultan Abdulkareem , Asikin-Mijan Nurul , Obeas Laith , Islam Aminul , Mansir Nasar , Teo Siow , Razali Siti , Nassar Maadh , Mohamad Surahim , Taufiq-Yap Yun
Published: May 2022

Originating from deoxygenation (DO) technology, green diesel was innovated in order to act as a substitute for biodiesel, which contains unstable fatty acid alkyl ester owing to the existence of oxygenated species. Green diesel was manufactured following a process of catalytic DO of sludge palm oil (SPO). An engineered Mn(0.5%)-Mo(0.5%)/AC catalyst was employed in a hydrogen-free atmosphere. The influence of Manganese (Mn) species (0.1–1 wt.%) on DO reactivity and the dissemination of the product were examined. The Mn(0.5%)-Mo(0.5%)/AC formulation gave rise to a superior harvest of approximately 89% liquid hydrocarbons; a higher proportion of diesel fraction selectivity n-(C15+C17) was obtained in the region of 93%. Where acid and basic active sites were present on the Mn(0.5%)-Mo(0.5%)/AC catalyst, decarboxylation and decarbonylation reaction mechanisms of SPO to DO were enhanced. Evidence of the high degree of stability of the Mn(0.5%)-Mo(0.5%)/AC catalyst during five continuous runs was presented, which, in mild reaction conditions, gave rise to a consistent hydrocarbon harvest of >72% and >94% selectivity for n-(C15+C17).
Neuropharmacological and Antidiarrheal Potentials of Duabanga grandiflora (DC.) Walp. Stem Bark and Prospective Ligand–Receptor Interactions of Its Bioactive Lead Molecules
Authors: Jahan Israt , Khan Mohammad Forhad , Sayeed Mohammed Abu , Arshad Laiba , Hossen Md. Amjad , Jakaria Md. , Ağagündüz Duygu , Haque Md. Areeful , Capasso Raffaele
Published: May 2022

Duabanga grandiflora (DC.) Walp. is an ethnomedicinally significant plant used to treat various illnesses, but there is little scientific evidence to support its use. This study explored the pharmacological activities of methanol extract of D. grandiflora stem barks (MEDG) through in vivo approaches in Swiss albino mice and a computer-aided molecular approach. The forced swimming test (FST), tail suspension test (TST), elevated plus maze (EPM), and hole board test (HBT) were used to determine anti-depressant and anxiolytic activity in experimental mice. In addition, anti-diarrheal studies were performed using castor oil-induced diarrhea, castor oil-induced enter pooling, and the charcoal-induced gastrointestinal motility test. MEDG showed substantial depletions in the immobility times in both FST and TST after treatment with the MEDG extract, whereas moderate anxiolytic activity was manifested at a higher dose (400 mg/kg) compared with the control. Correspondingly, MEDG extract revealed a significant reduction in wet feces and decreased the small intestinal transit of charcoal meal in castor oil-induced diarrhea and charcoal-induced gastrointestinal motility test. In the computer-aided molecular approaches, vanillin displayed a promising binding score for both anxiolytic and anti-diarrheal activities, while duabanganal C showed a promising score for the anti-depressant activity. The present experimental findings along with a computer-aided model conclude that MEDG could be a possible Phyto therapeutic agent with potential anti-depressant, anxiolytic and anti-diarrheal activity.
Physicochemical and Biological Evaluation of Chitosan-Coated Magnesium-Doped Hydroxyapatite Composite Layers Obtained by Vacuum Deposition
Authors: Predoi Daniela , Ciobanu Carmen Steluta , Iconaru Simona Liliana , Raaen Steinar , Badea Monica Luminita , Rokosz Krzysztof
Published: May 2022

In the present work, the effectiveness of vacuum deposition technique for obtaining composite thin films based on chitosan-coated magnesium-doped hydroxyapatite Ca10−xMgx(PO4)6 (OH)2 with xMg = 0.025 (MgHApCh) was proved for the first time. The prepared samples were exposed to three doses (0, 3, and 6 Gy) of gamma irradiation. The MgHApCh composite thin films nonirradiated and irradiated were evaluated by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) studies. The biological evaluation of the samples was also presented. All the results obtained from this study showed that the vacuum deposition method allowed for obtaining uniform and homogeneous layers. Fine cracks were observed on the MgHApCh composite thin films’ surface after exposure to a 6 Gy irradiation dose. Additionally, after gamma irradiation, a decrease in Ca, P, and Mg content was noticed. The MgHApCh composite thin films with doses of 0 and 3 Gy of gamma irradiation showed a cellular viability similar to that of the control. Samples with 6 Gy doses of gamma irradiation did not cause significantly higher fibroblast cell death than the control (p > 0.05). On the other hand, the homogeneous distribution of pores that appeared on the surface of coatings after 6 Gy doses of gamma irradiation did not prevent the adhesion of fibroblast cells and their spread on the coatings. In conclusion, we could say that the thin films could be suitable both for use in bone implants and for other orthopedic and dentistry applications.
Open Surgery including Lymphadenectomy without Adjuvant Therapy for Uterine-Confined Intermediate- and High-Risk Endometrioid Endometrial Carcinoma
Authors: Otsuka Isao , Matsuura Takuto , Mitani Takahiro , Otsuka Koji , Kanamoto Yoshihisa
Published: May 2022

Minimally invasive surgery may not be an appropriate surgical approach in intermediate- and high-risk endometrial carcinoma, even though adjuvant therapy is given. The objective of this study was to evaluate the results of open surgery including lymphadenectomy without adjuvant therapy in patients with uterine-confined intermediate- and high-risk endometrioid endometrial carcinoma. Two hundred fifty-six patients with uterine-confined endometrioid endometrial carcinoma were treated with open surgery, including pelvic with or without para-aortic lymphadenectomy. Of the 81 patients with uterine-confined intermediate- or high-risk disease, 77 were treated with systematic lymphadenectomy without adjuvant therapy. Seven patients developed recurrence, comprising 5.5% (3/55) and 18.2% (4/22) of the intermediate- and high-risk patients, respectively. The time to recurrence was 1–66 months. The sites of recurrence were the vaginal apex (n = 2), lung (n = 2), vaginal sidewall (n = 1), pelvic lymph nodes (n = 1), and para-aortic to supraclavicular nodes (n = 1). Of these, five patients were alive without disease after salvage treatment, but two understaged high-risk patients died of disease. The five-year disease-specific survival rates of intermediate- and high-risk patients were 100% and 90%, respectively. The present study indicated that patients with uterine-confined intermediate- and high-risk endometrioid endometrial carcinoma had excellent survival when treated with open surgery, including lymphadenectomy alone. The safety of omitting adjuvant therapy should be evaluated in prospective randomized trials comparing open surgery with minimally invasive surgery.
Potential Distribution and Medicinal Uses of the Mexican Plant Cuphea aequipetala Cav. (Lythraceae)
Authors: Garibay-Castro Luis Rafael , Gutiérrez-Yurrita Pedro Joaquín , López-Laredo Alma Rosa , Hernández-Ruíz Jesús , Trejo-Espino José Luis
Published: May 2022

Carrying out studies that lead us to obtain information on both the cultural and biological heritage of a locality, region, or country allows us to create appropriate strategies for the conservation of biocultural diversity. In this context, the objective of this study was to model the potential distribution of Cuphea aequipetala Cav. within the Mexican territory, to identify the main environmental variables that delimit its habitat, and to obtain information from traditional knowledge through the medicinal uses that the inhabitants of nearby communities give to the plant. Potential distribution modeling was performed with MaxEnt together with 19 bioclimatic variables of Worldclim plus three variables closely related to the habitat of the species. Data on its presence were obtained in situ within the Lagunas de Zempoala National Park and from iNaturalist. Information on the medicinal uses of the plant was obtained through semi-structured surveys with people who were in continuous contact with it. The potential range of C. equipetala Cav. was 3205.63 km2, which represents 0.16% of the Mexican territory. The altitude, precipitation in the driest period, average temperature of the warmest quarter, and average temperature of the driest quarter were the variables that had the greatest effects on the potential distribution (49%), and these factors mainly delimited the suitability of the habitat. C. aequipetala Cav. is still used in traditional medicine, mainly for conditions related to cancer, shocks, and inflammation. Finally, it was found that the potential distribution coincided with the states of the country where its medicinal use was reported. This information is important, since it constitutes the basis for performing actions targeting the conservation of this species of medicinal relevance. For example, potential distribution areas can be integrated into habitat restoration and conservation plans to prevent anthropogenic activities, such as felling, that directly affect the habitat. This information can also be used as a reference to promote the preservation of medicinal uses among the young population of the distribution areas.
Quantum Estimates for Different Type Intequalities through Generalized Convexity
Authors: Almutairi Ohud Bulayhan
Published: May 2022

This article estimates several integral inequalities involving (h−m)-convexity via the quantum calculus, through which Important integral inequalities including Simpson-like, midpoint-like, averaged midpoint-trapezoid-like and trapezoid-like are extended. We generalized some quantum integral inequalities for q-differentiable (h−m)-convexity. Our results could serve as the refinement and the unification of some classical results existing in the literature by taking the limit q→1−.
Energy Transition Pathways for Deep Decarbonization of the Greater Montreal Region: An Energy Optimization Framework
Authors: Aliakbari Sani Sajad , Maroufmashat Azadeh , Babonneau Frédéric , Bahn Olivier , Delage Erick , Haurie Alain , Mousseau Normand , Vaillancourt Kathleen
Published: May 2022

More than half of the world’s population live in cities, and by 2050, it is expected that this proportion will reach almost 68%. These densely populated cities consume more than 75% of the world’s primary energy and are responsible for the emission of around 70% of anthropogenic carbon. Providing sustainable energy for the growing demand in cities requires multifaceted planning approach. In this study, we modeled the energy system of the Greater Montreal region to evaluate the impact of different environmental mitigation policies on the energy system of this region over a long-term period (2020–2050). In doing so, we have used the open-source optimization-based model called the Energy–Technology–Environment Model (ETEM). The ETEM is a long-term bottom–up energy model that provides insight into the best options for cities to procure energy, and satisfies useful demands while reducing carbon dioxide (CO2) emissions. Results show that, under a deep decarbonization scenario, the transportation, commercial, and residential sectors will contribute to emission reduction by 6.9, 1.6, and 1 million ton CO2-eq in 2050, respectively, compared with their 2020 levels. This is mainly achieved by (i) replacing fossil fuel cars with electric-based vehicles in private and public transportation sectors; (ii) replacing fossil fuel furnaces with electric heat pumps to satisfy heating demand in buildings; and (iii) improving the efficiency of buildings by isolating walls and roofs.
SiC and FeCrAl as Potential Cladding Materials for APR-1400 Neutronic Analysis
Authors: Alrwashdeh Mohammad , Alameri Saeed A.
Published: May 2022

The aim of this study is to investigate the potential improvement of accident-tolerant fuels in pressurized water reactors for replacing existing reference zircaloy (Zr) fuel-cladding systems. Three main strategies for improving accident-tolerant fuels are investigated: enhancement of the present state-of-the-art zirconium fuel-cladding system to improve oxidation resistance, replacement of the current referenced fuel-cladding system material with an alternative high-performance oxidation-resistant cladding, and replacement of the current fuel with alternative fuel forms. This study focuses on a preliminary analysis of the neutronic behavior and properties of silicon carbide (SiC)-fuel and FeCrAl cladding systems, which provide a better safety margin as accident-tolerant fuel systems for pressurized water reactors. The typical physical behavior of both cladding systems is investigated to determine their general neutronic performance. The multiplication factor, thermal neutron flux spectrum, 239Pu inventory, pin power distribution, and radial power are analyzed and compared with those of a reference Zr fuel-cladding system. Furthermore, the effects of a burnable poison rod (Gd2O3) in different fuel assemblies are investigated. SiC cladding assemblies present a softer neutron spectrum and a lower linear power distribution compared with the conventional Zr-fuel-cladding system. Additionally, the SiC fuel-cladding system exhibits behaviors that are consistent with the neutronic behavior of conventional Zr fuel-cladding systems, thereby affording greater economic and safety improvements.
Performance Assessment of an Air-Type BIPVT Collector with Perforated Baffles through Indoor and Outdoor Experiments
Authors: Kim Jin-Hee , Yu Ji-Suk , Gaucher-Loksts Erin , Roy Benjamin , Delisle Véronique , Kim Jun-Tae
Published: May 2022

The performance of air-type PVT and BIPVT collectors has been extensively studied. As a system that generates heat and power, PVT collector testing has some particularities especially when using air as a heat recovery fluid and a building-integrated design (BIPVT). The electrical and thermal experimental performance of such collectors are currently being evaluated using in-house methods or PV and/or solar thermal collector standards. The use of a wide range of methods, testing conditions and experimental setups makes it difficult not only to compare the performance of different designs, but also to have confidence in the results obtained. This study evaluates the performance of an air-type BIPVT collector with in-channel perforated baffle plates for heat transfer enhancement designed for a building-integrated façade. As part of a joint research project between Korea and Canada, the proposed collector’s performance was evaluated through indoor (Canada) and outdoor experiments (Korea). Limited comparison of the results obtained with the two testing methods could be performed due to differences in environmental testing conditions, BIPVT collector area and experimental setup. Nevertheless, the limited measurement points under comparable testing conditions indicate that the results from the indoor and outdoor experiments have a similar trend. A comparison between the studied collector having a full PV absorber and a BIPVT collector with a hybrid PV/solar thermal collector absorber using a similar indoor experimental setup and testing conditions was performed. It showed that under still air conditions, for an irradiance level of approximately 820 W/m2 and with a low flow rate, the BIPVT collector with a hybrid PV/solar thermal absorber has a thermal and electrical efficiency of 25.1% and 5.9%, respectively. Under similar conditions, the BIPVT collector with a full PV absorber has a thermal efficiency of 23.9% and an electrical efficiency of 13.5%. At higher flowrates, both units have similar thermal efficiencies, however, the BIPVT collector with a PV absorber remains with an electrical efficiency that is more than double that of the unit with a hybrid PV/solar thermal absorber.
Multi-Dimensional Evaluation Framework for the Sustainable Development of Forest Health Bases and Site Selection for Application in China
Authors: Fan Chenjing , Zhou Lingling , Gai Zhenyu , Shen Shiguang , Liu Chu , Li Shiqi
Published: May 2022

In the context of global aging, people’s awareness of health is deepening, and the rapid economic development has drawn widespread attention to the health tourism industry. As a way of experiencing health, forest health tourism is becoming increasingly favored, and the site selection and construction of forest health bases (FHBs) have also developed accordingly. To ensure sustainability in the process of the site selection and construction of FHBs, the suitability of regional development and the relative coordination of the market, environment, and resource levels should be considered. Although there have been numerous studies on sustainable forestry management, a comprehensive sustainability assessment framework based on development suitability and coordination in three dimensions is needed to guide the site selection and the construction of FHBs. The following tasks were carried out in this study: (1) based on market sustainability goals, environmental optimization goals, and ecological resource sustainability goals, a comprehensive sustainability evaluation framework for development suitability indicators and coordination indicators in three dimensions was established; (2) via the use of this framework, the construction potential of FHBs in 41,636 towns in China was evaluated; the evaluation results show that the towns in Anhui, Jiangxi, Guangdong, Guangxi, Fujian, Zhejiang, Hunan, Hubei, Guizhou, and other provinces of China generally have superior conditions for the development of FHBs; (3) a multi-dimensional comprehensive analysis of FHB site selection sustainability based on development suitability and coordination was carried out for four batches of approved pilots. The comprehensive analysis results demonstrate the worsening evaluation results of the four batches. The proposed framework can provide a reference for FHB development policies for countries worldwide.
Linking Changes in Fatty Acid Composition to Postharvest Needle Abscission Resistance in Balsam Fir Trees
Authors: MacDonald Gaye E. , Lada Rajasekaran R. , Caldwell Claude D. , Udenigwe Chibuike C. , MacDonald Mason T.
Published: May 2022

Balsam fir needle retention and fatty acid profile changes due to cold acclimation throughout autumn, but little is known about the relationship between these two phenomena. The objective was to examine differences in FAs in contrasting needle abscission resistant balsam fir genotypes throughout autumn and early winter. Branches from genotypes with low and high needle abscission resistance (NAR) were collected from September to January and analyzed for FA composition. High NAR genotypes retained needles 120–130% longer than low NAR genotypes and NAR increased through autumn in both genotypes. There was approximately a 3:1 ratio of unsaturated: saturated FAs, which increased by 4% in favor of unsaturated fatty acids through autumn. Palmitic, palmitoleic, and linolenic acid content was significantly higher in high NAR versus low NAR genotypes; arachidic, oleic, linoleic, pinolenic, coniferonic, icosadienoic, and sciadonic acids were lower in high NAR genotypes versus low. Linolenic acid was of particular interest because it tended to decrease throughout autumn, to the point that high NAR genotypes were significantly lower in linolenic acid than low NAR genotypes in January. These changes may be linked to an increase in abscisic acid and/or jasmonic acid synthesis depleting linolenic acid stores and promoting postharvest needle abscission resistance.
Some Methods for the Degradation-Fragility Degree Determination and for the Consolidation of Treatments with Paraloid B72 of Wood Panels from Icon-Type Heritage Objects
Authors: Avram Anamaria , Ionescu Constantin Ștefan , Lunguleasa Aurel
Published: May 2022

The main objective of this paper is to develop methods for assessing the deterioration of wooden panels of iconic heritage objects and the effectiveness of consolidation treatments, methods that are easy to apply to the field of wood restoration. During the research, four evaluation methods were identified, respectively: the density method, the excessive porosity method, the Brinell hardness method, and the Mark hardness method. Each method was exemplified on five wooden panels (icons), and when needed, degraded specimens were used and/or treated with Paraloid B72. One of the main conclusions of the research is that, although all methods are minimally invasive and do not require cutting of these heritage objects, the applicability of each is done depending on the type of degradation, often requiring a combined analysis between two or several methods. Additionally, the classification of the cultural good in one of the five degrees of embrittlement-degradation help to design a technological flow regarding the treatments of consolidation/restoration of the heritage object.
Habitat Characteristics of Magnolia Based on Spatial Analysis: Landscape Protection to Conserve Endemic and Endangered Magnolia sulawesiana Brambach, Noot., and Culmsee
Authors: Kinho Julianus , Arini Diah Irawati Dwi , Abdulah Lutfy , Susanti Ruliyana , Irawan Arif , Yulianti Mira , Subarudi Subarudi , Imanuddin Rinaldi , Wardani Marfuah , Denny Denny , Kalima Titi , Hardjana Asef Kurniyawan , Susilo Adi , Heriansyah Ika , Tampang Ana
Published: May 2022

Based on habitat preferences, in this study, we investigated the spatial distribution of the Magnolia genus in the northern part of Sulawesi. Habitat characteristics, especially temperature, precipitation, and topography, were determined using spatial analysis. The temperature and precipitation datasets were obtained from WorldClim BIO Variables V1, and topographical data were obtained from the Google Earth Engine. Data collection began in 2008–2009 and was completed in 2019–2020. In total, we analyzed 786 waypoints. The genus distribution was then predicted based on the most suitable habitat characteristics and mapped spatially. This study confirmed that Magnolia spp. distribution is affected by the annual temperature range, precipitation seasonality, and elevation. We discovered endemic and endangered species, Magnolia sulawesiana Brambach, Noot., and Culmsee, that were previously distributed exclusively in the central part of Sulawesi. Five waypoints of the endemic species were found in the conservation area of the Gunung Ambang Nature Reserve and on the border of Bogani Nani Wartabone Nation Park. In general, M. sulawesiana is distributed at higher elevations than other Magnolia species. This study provides a scientific basis for forest officers to develop in-situ and ex-situ conservation strategies and landscape protection measures to maintain the sustainable use of the genus, especially the sustainability of endemic species.
Broad-Leaved Tree Growth Modulated by Industrial Air Pollution in the Northern Romania (Baia Mare Region)
Authors: Sidor Cristian Gheorghe , Cuciurean Cosmin Ilie , Popa Ionel , Leca Ștefan , Vlad Radu , Badea Ovidiu
Published: May 2022

Atmospheric pollutants over the last century have led to increased negative impacts on the environment, especially on forest ecosystems. In the Baia Mare region of Romania, the influence of pollution on the neighboring forests of the municipality has been reported since 1970, and its negative effects have been reported mainly in the form of reduced tree growth, which implies significant losses of wood biomass. The objective of this study is to analyze the temporal and spatial effect of industrial pollution on the auxological processes of beech trees in this region. Quantification of auxological changes was performed by analyzing the resilience, recovery and resistance indices. The most intense negative effect of local pollution with heavy metal dusts, sulfur and nitrogen oxides, and sulfuric acid vapors, on the auxological processes of beech trees was found in the period 1960–1990, with a maximum in the period 1970–1980, when the mining activity was at its highest intensity. Beech trees responded to the negative effect of pollution by significantly reducing their growth during the period affected by local pollution, and after 1990 they resumed their auxological activity close to normal. In addition, it was noted that the index that best captures the effect of pollution over time is the resilience index. Tree growth resilience, recovery, and resistance assessment and analysis significantly contributes to our understanding of trees response to environment pollution more broadly creating also the base for strategic planning initiatives with valuable insight into the efforts of making the forests more resilient and resistant.
Multifidelity Analysis of a Solo Propeller: Entropy Rise Using Vorticity Dynamics and Kinetic Energy Dissipation
Authors: Siddappaji Kiran , Turner Mark
Published: May 2022

Propellers for electric aviation are used in solo- and multirotor applications. Multifidelity analysis with reduced cycle time is crucial to explore several designs for energy minimization and range maximization. A low-fidelity design tool, py_BEM, is developed for design and analysis of a reverse-engineered solo 2-bladed propeller using blade-element momentum theory with physics enhancements including local Reynolds number effect, boundary-layer rotation, airfoil polar at large AoAs and stall delay. Spanwise properties from py_BEM are converted into 3D blade geometry using T-Blade3. S809 and NACA airfoil polar are utilized, obtained by XFOIL. Lift, drag, performance losses, wake analysis, comparison of 3D steady CFD with low fidelity tool, kinetic energy dissipation, entropy and exergy through irreversibility are analyzed. Spanwise thrust and torque comparison between low and high fidelity reveals the effect of blade rotation on the polar. Vorticity dynamics and boundary-vorticity flux methods describe the onset of flow separation and entropy rise. Various components of drag and loss are accounted. The entropy rise in the boundary layer and downstream propagation and mixing out with freestream are demonstrated qualitatively. Irreversibility is accounted downstream of the rotor using the second-law approach to understand the quality of available energy. The performance metrics are within 5% error for both fidelities.
The Evolution of Cooperation in Two-Dimensional Mobile Populations with Random and Strategic Dispersal
Authors: Weishaar Kyle , Erovenko Igor V.
Published: May 2022

We investigate the effect of the environment dimensionality and different dispersal strategies on the evolution of cooperation in a finite structured population of mobile individuals. We consider a population consisting of cooperators and free-riders residing on a two-dimensional lattice with periodic boundaries. Individuals explore the environment according to one of the four dispersal strategies and interact with each other via a public goods game. The population evolves according to a birth–death–birth process with the fitness of the individuals deriving from the game-induced payouts. We found that the outcomes of the strategic dispersal strategies in the two-dimensional setting are identical to the outcomes in the one-dimensional setting. The random dispersal strategy, not surprisingly, resulted in the worst outcome for cooperators.
Recent Advances in Understanding the Structures of Translesion Synthesis DNA Polymerases
Authors: Ling Justin A. , Frevert Zach , Washington M. Todd
Published: May 2022

DNA damage in the template strand causes replication forks to stall because replicative DNA polymerases are unable to efficiently incorporate nucleotides opposite template DNA lesions. To overcome these replication blocks, cells are equipped with multiple translesion synthesis polymerases that have evolved specifically to incorporate nucleotides opposite DNA lesions. Over the past two decades, X-ray crystallography has provided a wealth of information about the structures and mechanisms of translesion synthesis polymerases. This approach, however, has been limited to ground state structures of these polymerases bound to DNA and nucleotide substrates. Three recent methodological developments have extended our understanding of the structures and mechanisms of these polymerases. These include time-lapse X-ray crystallography, which allows one to identify novel reaction intermediates; full-ensemble hybrid methods, which allow one to examine the conformational flexibility of the intrinsically disordered regions of proteins; and cryo-electron microscopy, which allows one to determine the high-resolution structures of larger protein complexes. In this article, we will discuss how these three methodological developments have added to our understanding of the structures and mechanisms of translesion synthesis polymerases.
Identification of Pathogenicity Loci in Magnaporthe oryzae Using GWAS with Neck Blast Phenotypic Data
Authors: Myint Nyein Nyein Aye , Korinsak Siripar , Chutteang Cattleya , Laosatit Kularb , Thunnom Burin , Toojinda Theerayut , Siangliw Jonaliza L.
Published: May 2022

Magnaporthae oryzae (M. oryzae) is the most destructive disease of rice worldwide. In this study, one hundred and two isolates of M. oryzae were collected from rice (Oryzae sativa L.) from 2001 to 2017, and six rice varieties with resistance genes Pizt, Pish, Pik, Pib, and Pi2 were used in a genome-wide association study to identify pathogenicity loci in M. oryzae. Genome-wide association analysis was performed using 5338 single nucleotide polymorphism (SNPs) and phenotypic data of neck blast screening by TASSEL software together with haplotype block and SNP effect analysis. Twenty-seven significant SNPs were identified on chromosomes 1, 2, 3, 4, 5, 6, and 7. Many predicted genes (820 genes) were found in the target regions of six rice varieties. Most of these genes are described as putative uncharacterized proteins, however, some genes were reported related to virulence in M. oryzae. Moreover, this study revealed that R genes, Pik, Pish, and Pi2, were broad-spectrum resistant against neck blast disease caused by Thai blast isolate. Haplotype analysis revealed that the combination of the favorable alleles causing reduced virulence of isolates against IRBLz5-CA carrying Pi2 gene contributes 69% of the phenotypic variation in pathogenicity. The target regions and information are useful to develop marker-specific genes to classify blast fungal isolates and select appropriate resistance genes for rice cultivation and improvement.
A Study of the Genomic Variations Associated with Autistic Spectrum Disorders in a Russian Cohort of Patients Using Whole-Exome Sequencing
Authors: Gibitova Ekaterina A. , Dobrynin Pavel V. , Pomerantseva Ekaterina A. , Musatova Elizaveta V. , Kostareva Anna , Evsyukov Igor , Rychkov Sergey Y. , Zhukova Olga V. , Naumova Oxana Y. , Grigorenko Elena L.
Published: May 2022

This study provides new data on the whole-exome sequencing of a cohort of children with autistic spectrum disorders (ASD) from an underexplored Russian population. Using both a cross-sectional approach involving a control cohort of the same ancestry and an annotation-based approach involving relevant public databases, we explored exonic single nucleotide variants and copy-number variation potentially involved in the manifestation of ASD. The study results reveal new potential ASD candidate-variants found in the studied Russian cohort and show a high prevalence of common ASD-associated genomic variants, especially those in the genes known to be associated with the manifestation of intellectual disabilities. Our screening of an ASD cohort from a previously understudied population allowed us to flag at least a few novel genes (IGLJ2, FAM21A, OR11H12, HIP1, PRAMEF10, and ZNF717) regarding their potential involvement in ASD.
The Role of Emotional Intelligence in Engagement in Nurses
Authors: Pérez-Fuentes María del Carmen , Molero Jurado María del Mar , Gázquez Linares José Jesús , Oropesa Ruiz Nieves Fátima
Published: May 2022

Aware that engagement in the healthcare field needs high levels of emotional intelligence, we began this study to determine relationship between engagement and emotional intelligence in nurses. The objective of this study was to determine the explanatory value of the components of emotional intelligence for engagement in a sample of nurses. The final study sample was made up of 2126 working nursing professionals. Data was obtained by distributing, an ad hoc questionnaire was used to collect sociodemographic information, and to collect professional and employment information, the Utrecht Work Engagement Scale and the Reduced Emotional Intelligence Inventory for Adults were used. The results showed that nurses with higher levels of emotional intelligence also scored more highly in engagement, with the interpersonal factor being the greatest predictor of engagement. This study has significant practical implications for the creation of intervention programs and activities to improve the performance of nurses in the workplace.
Associations between Training Load and Well-Being in Elite Beach Soccer Players: A Case Report
Authors: Costa Júlio A. , Figueiredo Pedro , Prata Alberto , Reis Tiago , Reis Joana F. , Nascimento Luís , Brito João
Published: May 2022

The current case study aimed to quantify within-subjects correlations between training load and well-being in elite male beach soccer players. Data were obtained over three consecutive days during the preparation camp for the FIFA Beach Soccer World Cup Russia 2021. The session rating of perceived exertion (s-RPE) and external training load metrics using global positioning system (GPS) were recorded. Eleven players reported perceived well-being (sleep quality, fatigue, muscle soreness and stress) using a Likert scale (Hooper Index) before breakfast. Within-subjects correlation coefficients between workload and well-being were calculated. Workload metrics and perceived well-being indices were significantly lower on day three than on days one and two. The Hooper Index presented a very large positive correlation with s-RPE (r = 0.86 [0.67, 0.94], 95% confidence interval, CI), exposure time (r = 0.88 [0.71, 0.95]), total distance (r = 0.83 [0.60, 0.93]), high-speed distance (r = 0.77 [0.50, 0.91]), and number of sprints (r = 0.75 [0.47, 0.90]). Sleep quality presented a moderate to large positive correlation with s-RPE (r = 0.51 [0.11, 0.77]), exposure time (r = 0.50 [0.10, 0.76]), high-speed distance (r = 0.53 [0.15, 0.78]), number of sprints (r = 0.62 [0.28, 0.83]) and total distance (r = 0.41 [0.18, 0.78]). Fatigue presented a large to very large positive correlation with s-RPE (r = 0.85 [0.66, 0.94]), exposure time (r = 0.90 [0.78, 0.96]), total distance (r = 0.86 [0.68, 0.94]), high-speed distance (r = 0.65 [0.31, 0.84]) and number of sprints (r = 0.56 [0.18, 0.79]). Muscle soreness presented a large to very large positive correlation with s-RPE (r = 0.79 [0.56, 0.91]), exposure time (r = 0.83 [0.62, 0.93]), total distance (r = 0.81 [0.59, 0.92]), high-speed distance (r = 0.75 [0.47, 0.89]) and number of sprints (r = 0.59 [0.22, 0.81]). Overall, workload presented a meaningful correlation with perceived well-being indices in elite male beach soccer players during a training camp. These findings suggest that workload metrics and perceived well-being indices can be implemented into the daily routine of an elite beach soccer team, which may assist coaches, sports scientists, and practitioners in better preparing players for beach soccer competitions.
The Spatiotemporal Evolutionary Trend and Driving Factors of the Coupling Coordinated Development between Regional Green Finance and Ecological Environment
Authors: Shi Tao
Published: May 2022

Based on the 30 inland provincial samples of China from 2003 to 2019, this article analyzes the evolutionary characteristics of the coupling coordination between green finance and the ecological environment (CCFE) using ArcGIS 10.5 software and employs the spatial Durbin model to analyze the driving factors of the CCFE. The results can be concluded as follows: (1) the CCFE of China is at a moderately low level, with a fluctuating upward trend. Spatially, it presents a spatial distribution pattern—higher in the east and lower in other regions. In terms of types, the regions of the CCFE are more in primary coordination and basic un-coordination and less in moderate un-coordination and moderate coordination. There are more regions of the green finance lagged type, and relatively few regions have achieved the financial ecological synchronization type. (2) The CCFE hotspots are concentrated in the Pearl River Delta, with a spatial “increase–decrease” development trend. Additionally, the CCFE cold spots are concentrated in the upper Yellow River Basin, with a relatively stable spatial scope. (3) The CCFE shows a positive spillover effect and accumulative delivery effect in the economic geospatial space. The population urbanization rate and the number of granted patent applications have a significant positive impact on the CCFE, and the percentage of secondary industries to GDP has a negative impact accordingly. Spatially, the percentage of secondary industries to GDP and the number of granted patent applications of nearby provinces in the economic geospatial space have a negative impact on the local CCFE. (4) The impact and spatial effect of different factors on the CCFE are obviously different. Finally, policy implications on the coordinated development of green finance and the ecological environment are also made.
Comparison of Brazilian Social Interest Housing Projects Considering Sustainability
Authors: Vitorio Junior Paulo Cezar , Yepes Víctor , Kripka Moacir
Published: May 2022

Considering the importance of the development of new housing projects, the purpose of this research is to provide a model oriented to the identification of the most sustainable alternative in single-family housing projects of social interest from the perspective of life cycle thinking (LCT) and the analytical hierarchical process (AHP). A ceramic masonry project and a concrete masonry project were evaluated. In the environmental dimension, the results showed that the ceramic masonry project had more significant environmental impacts and greater damage to human health and the availability of resources and ecosystems. In the social dimension, it was found that there are discrepancies between the salaries in the construction supply chain and that the concrete masonry project had better social characteristics than the ceramic masonry project. The economic dimension revealed that the concrete masonry project was more attractive. Relating the environmental, social, and economic dimensions’ results, through the combination of LCT and AHP, it was found that the concrete masonry project presented a combination of more sustainable characteristics than the ceramic masonry project in the majority of the results. Among the implications of the study carried out here is the advancement of sustainability applied to the construction sector.
Multi-Scenario Simulation and Trade-Off Analysis of Ecological Service Value in the Manas River Basin Based on Land Use Optimization in China
Authors: Du Yongjun , Li Xiaolong , He Xinlin , Li Xiaoqian , Yang Guang , Li Dongbo , Xu Wenhe , Qiao Xiang , Li Chen , Sui Lu
Published: May 2022

Rapid socio-economic development has had a significant impact on land use/cover (LULC) changes, which bring great pressure to the ecological environment. LULC changes affect ecosystem services by altering the structure and function of ecosystems. It is of great significance to reveal the internal relationship between LULC changes and ecosystem service value (ESV) for the protection and restoration of ecological environments. In this study, based on the spatial and temporal evolution of ecological service values in the Manas River basin from 1980 to 2020 and considering ecological and economic benefits, we coupled the gray multi-objective optimization model (GMOP) and patch-generating land-use simulation (PLUS) model (GMOP–PLUS model) to optimize the LULC structure under three scenarios (a natural development scenario, ND; ecological priority development scenario, (EPD); and balanced ecological and economic development scenario, EED) in 2030, and analyzed the trade-offs and synergies in the relationships among the four services. We found that from 1980 to 2020, farmland and construction land expanded 2017.90 km2 and 254.27 km2, respectively, whereas the areas of grassland and unused land decreased by 1617.38 km2 and 755.86 km2, respectively. By 2030, the trend of LULC changes will be stable under the ND scenario, the area of ecological land will increase by 327.42 km2 under the EPD scenario, and the area of construction land will increase most under the EED scenario, reaching 65.01 km2. From 1980 to 2020, the ESV exhibited an upward trend in the basin. In 2030, the ESV will increase by 7.18%, 6.54%, and 6.04% under the EPD, EED, and ND scenarios, respectively. The clustering of the four services is obvious in the desert area and around the water system with “low–low synergy” and “high–high synergy”; the plain area and mountainous area are mainly “high–low trade-off” and “low–high trade-off” relationships. This paper provides a scientific reference for coordinating economic development and ecological protection in the basin. It also provides a new technical approach to address the planning of land resources in the basin.