bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary Structural Characterization of Selenium Nanoparticle Composites Modified by Astragalus Polysaccharide and the Cytotoxicity Mechanism on Liver Cancer Cells
Authors: Ji Haiyu , Lou Xiaowei , Jiao Jianshuang , Li Yang , Dai Keyao , Jia Xiaoyu
Published: February 2023

Astragalus alcohol soluble polysaccharide (AASP) could present superior water solubility and antitumor activity with high concentration. Selenium nanoparticles (SeNPs) have received growing attention in various fields, but their unstable property increases the application difficulties. In the present study, functionalized nano-composites (AASP−SeNPs) were synthesized through SeNPs using AASP (average molecular weight of 2.1 × 103 Da) as a surface modifier, and the preliminary structural characteristics and inhibitory mechanism on liver cancer (HepG2) cells were investigated. Results showed that AASP−SeNPs prepared under a sodium selenite/AASP mass ratio of 1/20 (w/w) were uniformly spherical with a mean grain size of 49.80 nm and exhibited superior dispersivity and stability in water solution. Moreover, the composites could dose-dependently inhibit HepG2 cell proliferation and induce apoptosis through effectively regulating mitochondria-relevant indicators including ΔΨm depletion stimulation, intracellular ROS accumulation, Bax/Bcl-2 ratio improvement, and Cytochrome c liberation promotion. These results provide scientific references for future applications in functional food and drug industries.
Meroterpenoids with Immunosuppressive Activity from Edible Fungus Craterellus odoratus
Authors: Dai Quan , Pang Li-Ting , Zhang Fa-Lei , Wang Gang-Qiang , Li Xue-Mei , Liu Ji-Kai , Feng Tao
Published: February 2023

Two unusual polyketide–sesquiterpene metabolites, craterodoratins T (1) and U (2), along with the known compound craterellin A (3), were isolated from the higher fungus Craterellus odoratus. The structures of isolated compounds were characterized based on nuclear magnetic resonance (NMR) and mass spectrum (MS) spectroscopic analysis, while the absolute configuration of the compounds was determined by theoretical NMR and electronic circular dichroism (ECD) calculations. Compound 1 possessed a rare structure with two aromatic groups. Compounds 1 and 3 showed immunosuppressive activity with IC50 values ranging from 5.516 to 19.953 μM.
Investigation of the Protective Effect for GcMAF by a Glycosidase Inhibitor and the Glycan Structure of Gc Protein
Authors: Kanie Yoshimi , Maegawa Yuya , Wei Yi , Kanie Osamu
Published: February 2023

O-linked α-N-acetylgalactosamine (α-GalNAc) in the Gc protein is essential for macrophage activation; thus, the GalNAc-attached form of Gc protein is called Gc macrophage activating factor (GcMAF). O-linked glycans in Gc proteins from human plasma mainly consist of trisaccharides. GcMAF is produced when glycans on the Gc protein are hydrolyzed by α-Sia-ase and β-Gal-ase, leaving an α-GalNAc. Upon hydrolysis of α-GalNAc present on GcMAF, the protein loses the macrophage-activating effect. In contrast, our synthesized pyrrolidine-type iminocyclitol possessed strong in vitro α-GalNAc-ase inhibitory activity. In this study, we examined the protective effects of iminocyclitol against GcMAF via inhibition of α-GalNAc-ase activity. Detailed mass spectrometric analyses revealed the protective effect of the inhibitor on GcMAF. Furthermore, structural information regarding the glycosylation site and glycan structure was obtained using tandem mass spectrometric (MS/MS) analysis of the glycosylated peptides after tryptic digestion.
Enhanced Physicochemical Stability of the L-DOPA Extract of Mucuna pruriens Seeds by Adding Phyllanthus emblica
Authors: Vilairat Chayarit , Kobtrakul Khwanlada , Vimolmangkang Sornkanok
Published: February 2023

Levodopa (L-DOPA) is an essential drug for the treatment of Parkinson’s disease. Currently, L-DOPA can be produced by chemical synthesis and can also be found naturally in many herbs, especially Mucuna Pruriens (MP). According to clinical research, the MP extract containing L-DOPA for the treatment of Parkinson’s disease could reduce side effects more than the synthetic one. Unfortunately, MP extracts can be easily degraded. Changes in physical and chemical properties such as the appearance (color, melt, solid lump) and the reduction of L-DOPA content in the extract were commonly observed. Therefore, it is necessary to develop an extraction procedure to stabilize the extract of L-DOPA. This study attempted to enhance the extraction process by modifying the traditional acidification approach using hydrochloric acid, citric acid, or ascorbic acid. According to the stability test results, using Phyllanthus emblica water (PEW) as a solvent improved the preservative properties more than other solvents. The color of the PEW-MP powder changed slightly after 12 months of accelerated storage, but the amount of L-DOPA remained the highest (73.55%). Moreover, L-DOPA was only detected in MP and PEW-MP, but not PEW alone (the HPTLC chromatogram at Rf 0.48 and the HPLC chromatogram at Rt 6.0 min). The chemical profiles of PEW and L-DOPA observed in the chromatograms indicated that they are independently separated. As a result, they can be applied to a quality control process. Therefore, PEW was proven to be a powerful solvent for L-DOPA herbal extract that could be readily used as a raw material for herbal products.
Neuroprotective Effects of Savinin on LPS-Induced Neuroinflammation In Vivo via Regulating MAPK/NF-κB Pathway and NLRP3 Inflammasome Activation
Authors: Tang Siqi , Li Chunying , Suo Zongwu , Xu Yi , Wei Kaixin , Zhao Lei , Huang Hao , Liu Xiangqian , Liu Dongxu , Li Xiaojun
Published: February 2023

The traditional herb Eleutherococcus henryi Oliv. is commonly used to treat inflammatory conditions including rheumatism, arthritis, and hepatitis, as well as mental fatigue and amnesia, according to traditional Chinese medicine (TCM) theory. Savinin is a natural lignan obtained from the roots of E. henryi. The present study was undertaken to determine whether savinin can relieve LPS-induced neuroinflammation and if so, what the mechanism is. Groups of male C57BL/6 mice were administered savinin (5, 10, 20 mg/kg) and DEX (10 mg/kg) by gavage once daily for a continuous 7 days. On the 5th day of continuous pre-administration, LPS (2.5 mg/kg) was injected into the lateral ventricles of the mice for modeling 48 h. We found that treatment with savinin decreased the levels of neuroinflammatory cytokines and histopathological alterations dramatically. Consequently, it improved the LPS-induced neuroinflammatory response in mice. Furthermore, savinin inhibited the up-regulated expression of related proteins in the activated MAPK/NF-κB and NLRP3 inflammasome signaling pathways caused by LPS. Docking studies demonstrated the binding of savinin to three receptors (MAPK, NF-κB and NLRP3) using a well-fitting mode. These findings suggest that savinin may suppress neuroinflammation induced by LPS in vivo via modulating MAPK/NF-κB and NLRP3 signaling pathways.
Study on Thermal Effect of Aluminum-Air Battery
Authors: Cai Yajun , Tong Yunwei , Liu Yingjie , Li Xinyu , Chen Beiyang , Liu Feng , Zhou Baowei , Liu Yichun , Qin Zhenbo , Wu Zhong , Hu Wenbin
Published: February 2023

The heat released from an aluminum-air battery has a great effect on its performance and operating life during the discharge process. A theoretical model was proposed to evaluate the resulting thermal effect, and the generated heat was divided into the following sources: anodic aluminum oxidation reaction, cathodic oxygen reduction reaction, heat production against the battery internal resistance, and hydrogen-evolution reaction. Quantitative analysis was conducted on each part, showing that all heat production sources increased with discharge current density. It should be noted that the heat caused by hydrogen evolution accounted for the most, up to 90%. Furthermore, the regulation strategy for inhibiting hydrogen evolution was developed by addition of hybrid additives to the electrolyte, and the hydrogen-evolution rate was greatly reduced by more than 50% as was the generated heat. This research has important guidance for the thermal effect analysis of aluminum–air batteries, together with control of the thermal management process by inhibiting hydrogen evolution, thus promoting their practical application.
The Impact of Diet and Physical Activity on Psoriasis: A Narrative Review of the Current Evidence
Authors: Duchnik Ewa , Kruk Joanna , Tuchowska Aleksandra , Marchlewicz Mariola
Published: February 2023

Psoriasis is an inflammatory disease with strong genetic links and numerous features of autoimmunity that are also influenced by environment and lifestyle, including nutritional factors and physical activity (PA), with regards to the condition of patients. Recent reports in the field of nutrigenomics indicate a significant impact of nutrients in modulating microRNAs. However, few studies have evaluated the effect of nutritional systems and PA on treating psoriasis. This narrative review updates information regarding the current dietary recommendations for individuals with psoriasis and discusses the role of diet and PA in psoriasis prevention and treatment. Application of nutrigenetics in psoriasis therapy is also discussed. The PubMed and Google Scholar databases were searched using the MeSH terms for “nutrigenomics”, “dietetics”, “diet therapy”, “diet”, “physical activity”, and “exercise” in conjunction with the MeSH terms for “psoriasis” and “dermatology”. Evidence has shown that patients with psoriasis should have a personalized anti-inflammatory diet. Psoriasis patients are less physically active; most performed exercises of low-to-moderate intensity and were less likely to undertake regular exercise. Identifying nutrigenomic discoveries and the current lifestyle interventions associated with psoriasis can help physicians and physical therapists develop educational programs to manage and protect against the disease.
Nutrients and Dementia: Prospective Study
Authors: Takeuchi Hikaru , Kawashima Ryuta
Published: February 2023

The association of diet and nutrients with dementia risk is an interesting research topic. Middle-aged and older Europeans not diagnosed with dementia within two years of baseline were followed up and their data were analysed until 2021. The association between the nutrient quintiles measured by the web-based 24 h dietary and the risk of developing dementia was examined using a Cox proportional hazard model after adjusting for potential confounding factors. Approximately 160,000 subjects and 1200 cases were included in the analysis of each nutrient. A greater risk of dementia was associated with (a) no alcohol intake (compared with moderate to higher intake), (b) higher intake of total sugars and carbohydrates (compared with lower intake), (c) highest or lowest fat intake (compared with moderate intake), (d) quintiles of highest or lowest magnesium intake (compared with the quintile of the second highest intake), and (e) highest protein intake (compared with moderate intake). Overall, the present results are congruent with the importance of a moderate intake of certain nutrients.
Λ Polarization and Vortex Rings in Heavy-Ion Collisions at NICA Energies
Authors: Ivanov Yuri B. , Soldatov Alexei A.
Published: February 2023

We review recent studies of vortical motion and the resulting polarization of Λ hyperons in heavy-ion collisions at NICA energies, in particular, within the model of three-fluid dynamics (3FD). This includes predictions of the global Λ polarization and ring structures that appear in Au+Au collisions. The global Λ polarization in Au+Au collisions is calculated, including its rapidity and centrality dependence. The contributions of the thermal vorticity and meson-field term (proposed by Csernai, Kapusta, and Welle) to the global polarization are considered. The results are compared with data from recent STAR and HADES experiments. It is predicted that the polarization maximum is reached at sNN≈ 3 GeV if the measurements are performed with the same acceptance. It is demonstrated that a pair of vortex rings are formed, one at forward rapidities and another at backward rapidities, in ultra-central Au+Au collisions at sNN> 4 GeV. The vortex rings carry information about the early stage of the collision, in particular, the stopping of baryons. It is shown that these rings can be detected by measuring the ring observable RΛ, even in the midrapidity region at sNN= 5–20 GeV. At forward/backward rapidities, the RΛ signal is expected to be stronger. The possibility of observing the vortex-ring signal against the background of non-collective transverse polarization is discussed.
Taugt17b1 Overexpression in Trichoderma atroviride Enhances Its Ability to Colonize Roots and Induce Systemic Defense of Plants
Authors: Chi Shengqi , Xue Xiaoyu , Zhang Ronghuan , Zhang Li , Yu Jinfeng
Published: February 2023

Trichoderma atroviride, a soil fungus, has important applications in the biocontrol of plant diseases. Glycosyltransferases enhance the root colonization ability of Trichoderma spp. This study aimed to functionally characterize glycosyltransferase Taugt17b1 in T. atroviride. We investigated the effect of Taugt17b1 overexpression in T. atroviride H18-1-1 on its biocontrol properties, especially its ability to colonize roots. Our results demonstrated that the overexpression of the Taugt17b1 increases the T. atroviride colony growth rate, improves its root colonization ability, promotes the growth and activity of the defensive enzymatic system of plants, and prevents plant diseases. This study put forth a new role of T. atroviride glycosyltransferase and furthered the understanding of the mechanisms by which fungal biocontrol agents exert their effect.
Understanding Tick-Borne Encephalitis Virus Foci, a Tale of Two Mountains
Authors: Cabezas-Cruz Alejandro , Banović Pavle
Published: February 2023

What factors influence the formation and disappearance of tick-borne encephalitis virus (TBEV) foci [...]
Mechanisms of a Mycobacterium tuberculosis Active Peptide
Authors: Rao Komal Umashankar , Li Ping , Welinder Charlotte , Tenland Erik , Gourdon Pontus , Sturegård Erik , Ho James C. S. , Godaly Gabriela
Published: February 2023

Multidrug-resistant tuberculosis (MDR) continues to pose a threat to public health. Previously, we identified a cationic host defense peptide with activity against Mycobacterium tuberculosis in vivo and with a bactericidal effect against MDR M. tuberculosis at therapeutic concentrations. To understand the mechanisms of this peptide, we investigated its interactions with live M. tuberculosis and liposomes as a model. Peptide interactions with M. tuberculosis inner membranes induced tube-shaped membranous structures and massive vesicle formation, thus leading to bubbling cell death and ghost cell formation. Liposomal studies revealed that peptide insertion into inner membranes induced changes in the peptides’ secondary structure and that the membranes were pulled such that they aggregated without permeabilization, suggesting that the peptide has a strong inner membrane affinity. Finally, the peptide targeted essential proteins in M. tuberculosis, such as 60 kDa chaperonins and elongation factor Tu, that are involved in mycolic acid synthesis and protein folding, which had an impact on bacterial proliferation. The observed multifaceted targeting provides additional support for the therapeutic potential of this peptide.
Pharmacodynamic and Pharmacokinetic Drug Interactions between Fexuprazan, a Novel Potassium-Competitive Inhibitor, and Aspirin, in Healthy Subjects
Authors: Oh JungJin , Yang Eunsol , Jang In-Jin , Lee Hyejung , Yoo Hokyun , Chung Jae-Yong , Lee SeungHwan , Oh Jaeseong
Published: February 2023

Acid-reducing agents are commonly used for the prevention of aspirin-induced gastrointestinal complications such as peptic ulcers. As a novel potassium-competitive acid blocker, fexuprazan is expected to prevent aspirin-induced gastrointestinal complications. This randomized, open-label study aimed to evaluate the pharmacodynamic and pharmacokinetic interactions between aspirin and fexuprazan in healthy Koreans. Subjects randomized to the aspirin group received 500 mg aspirin in combination with 80 mg fexuprazan. For the fexuprazan group, fexuprazan 80 mg was administered alone and then in combination with aspirin 500 mg. Platelet aggregation inhibited by aspirin and the pharmacokinetic parameters of aspirin and fexuprazan were compared between monotherapy and combination therapy. A total of 22 subjects completed the study. The platelet aggregation-inhibitory activity and systemic exposure to aspirin were not significantly affected by fexuprazan coadministration. The systemic exposure of fexuprazan was decreased up to 20% by aspirin coadministration, which was not regarded as clinically meaningful considering the previously reported exposure–response relationship. In conclusion, there were no clinically relevant pharmacodynamic or pharmacokinetic interactions between aspirin and fexuprazan. This finding suggests the potential of fexuprazan for the prevention of aspirin-induced gastrointestinal complications, serving as a baseline for optimizing its therapeutic application with aspirin.
Measuring Creatinine Clearance Is the Most Accurate Way for Calculating the Proper Continuous Infusion Meropenem Dose for Empirical Treatment of Severe Gram-Negative Infections among Critically Ill Patients
Authors: Troisi Carla , Cojutti Pier Giorgio , Rinaldi Matteo , Laici Cristiana , Siniscalchi Antonio , Viale Pierluigi , Pea Federico
Published: February 2023

Assessment of glomerular filtration rate (GFR) is necessary for dose adjustments of beta-lactam that are excreted by the kidneys, such as meropenem. The aim of this study was to compare the daily dose of 24 h-continuous infusion (CI) meropenem when GFR was calculated by means of measured creatinine clearance (mCLCR) or estimated by the CKDEPI (eGFRCKDEPI), Cockcroft–Gault (eGFRCG), and MDRD (eGFRMDRD) equations. Adult critically ill patients who underwent therapeutic drug monitoring (TDM) for the assessment of 24 h-CI meropenem steady state concentration (Css) and for whom a 24 h-urine collection was performed were retrospectively enrolled. Meropenem clearance (CLM) was regressed against mCLCR, and meropenem daily dose was calculated based on the equation infusion rate = daily dose/CLM. eGFRCKDEPI, eGFRCG, and eGFRCKDEPI were regressed against mCLCR in order to estimate CLM. Forty-six patients who provided 133 meropenem Css were included. eGFRCKDEPI overestimated mCLCR up to 90 mL/min, then mCLCR was underestimated. eGFRCG and eGFRMDRD overestimated mCLCR across the entire range of GFR. In critically ill patients, dose adjustments of 24 h-CI meropenem should be based on mCLCR. Equations for estimation of GFR may lead to gross under/overestimates of meropenem dosages. TDM may be highly beneficial, especially for critically ill patients with augmented renal clearance.
BrCYP71A15 Negatively Regulates Hg Stress Tolerance by Modulating Cell Wall Biosynthesis in Yeast
Authors: Anwar Ali , Zhang Shu , Wang Lixia , He Lilong , Gao Jianwei
Published: February 2023

Over the past two decades, heavy metal pollution has been a common problem worldwide, greatly threatening crop production. As one of the metal pollutants, Mercury (Hg) causes damage to plant cells and reduces cellular and biochemical activities. In this study, we identified a novel cytochrome P450 family gene, BrCYP71A15, which was involved in Hg stress response in yeast. In Chinese cabbage, the BrCYP71A15 gene was located on chromosome A01, which was highly expressed in roots. Additionally, the expression level of BrCYP71A15 was induced by different heavy metal stresses, and the BrCYP71A15 protein exhibited a strong interaction with other proteins. Overexpression of BrCYP71A15 in yeast cells showed no response to a number of heavy metal stresses (Cu, Al, Co, Cd) in yeast but showed high sensitivity to Hg stress; the cells grew slower than those carrying the empty vector (EV). Moreover, upon Hg stress, the growth of the BrCYP71A15-overexpressing cells increased over time, and Hg accumulation in yeast cells was enhanced by two-fold compared with the control. Additionally, BrCYP71A15 was translocated into the nucleus under Hg stress. The expression level of cell wall biosynthesis genes was significantly influenced by Hg stress in the BrCYP71A15-overexpressing cells. These findings suggested that BrCYP71A15 might participate in HG stress tolerance. Our results provide a fundamental basis for further genome editing research and a novel approach to decrease Hg accumulation in vegetable crops and reduce environmental risks to human health through the food chain.
The Amelioration of Grazing through Physiological Integration by a Clonal Dune Plant
Authors: Evans Jonathan P. , Meckstroth Shelby , Garai Julie
Published: February 2023

Rhizomatous growth and associated physiological integration can allow a clonal dune species to potentially compensate for the selective removal of leaves associated with herbivory. Hydrocotyle bonariensis is a rhizomatous clonal plant species that is abundant in the coastal dune environments of the southeastern United States that are inhabited by large feral horse populations. H. bonariensis has been shown to integrate resources among ramets within extensive clones as an adaptation to resource heterogeneity in sandy soils. In this study, we hypothesized that clonal integration is a mechanism that promotes H. bonariensis persistence in these communities, despite high levels of herbivory by feral horses. In a field experiment, we used exclosures to test for herbivory in H. bonariensis over a four-month period. We found that feral horses utilized H. bonariensis as a food species, and that while grazing will suppress clonal biomass, H. bonariensis is able to maintain populations in a high grazing regime with and without competition present. We then conducted an experiment in which portions of H. bonariensis clones were clipped to simulate different levels of grazing. Half of the clones were severed to eliminate the possibility of integration. We found that after 12 weeks, the mean number of leaves and ramets increased as the grazing level increased, for integrated clones. Integrated clones had significantly increased biomass production compared to the severed equivalents. Our research suggests that rhizomatous growth and physiological integration are traits that allow clonal plant species to maintain populations and to tolerate grazing in coastal dune environments.
Canola with Stacked Genes Shows Moderate Resistance and Resilience against a Field Population of Plasmodiophora brassicae (Clubroot) Pathotype X
Authors: Tonu Nazmoon Naher , Wen Rui , Song Tao , Guo Xiaowei , Murphy Lee Anne , Gossen Bruce Dean , Yu Fengqun , Peng Gary
Published: February 2023

Genetic resistance is a cornerstone for managing clubroot (Plasmodiophora brassicae). However, when used repeatedly, a clubroot resistance (CR) gene can be broken rapidly. In this study, canola inbred/hybrid lines carrying one or two CR genes (Rcr1/CRaM and Crr1rutb) were assessed against P. brassicae pathotype X by repeated exposure to the same inoculum source under a controlled environment. Lines carrying two CR genes, either Rcr1 + Crr1rutb or CRaM + Crr1rutb, showed partial resistance. Selected lines were inoculated with a field pathotype X population (L-G3) at 5 × 106 resting spores/g soil, and all clubs were returned to the soil they came from six weeks after inoculation. The planting was repeated for five cycles, with diseased roots being returned to the soil after each cycle. The soil inoculum was quantified using qPCR before each planting cycle. All lines with a single CR gene were consistently susceptible, maintaining high soil inoculum levels over time. The lines carrying two CR genes showed much lower clubroot severity, resulting in a 10-fold decline in soil inoculum. These results showed that the CR-gene stacking provided moderate resistance against P. brassicae pathotype X, which may also help reduce the pathogen inoculum buildup in soil.
Growth, Flowering, and Fruit Production of Strawberry ‘Albion’ in Response to Photoperiod and Photosynthetic Photon Flux Density of Sole-Source Lighting
Authors: Park Yujin , Sethi Rashmi , Temnyk Stephanie
Published: February 2023

Beyond producing leafy greens, there is a growing interest in strawberry production on indoor vertical farms. Considering that sole-source lighting is one of the most important components for successful indoor crop production, we investigated how photosynthetic photon flux density (PPFD) and the photoperiod of sole-source lighting affected plant growth, flowering, and fruit production in strawberry ‘Albion.’ Bare-rooted strawberry plants were grown in deep water culture hydroponics inside an indoor vertical farm at 21 °C under white + blue + red light-emitting diodes at a PPFD of 200, 300, or 450 µmol∙m−2∙s−1 with a 12-h or 16-h photoperiod. Under both photoperiods, increasing PPFD from 200 to 450 µmol∙m−2∙s−1 linearly increased crown diameter by 18–64%, shoot fresh and dry mass by 38–80%, and root fresh and dry mass by 19–48%. Under a PPFD ≥ 300 µmol∙m−2∙s−1, root fresh and dry biomass increased by 95–108% and 41–44%, respectively, with an increasing photoperiod from 12 to 16 h. In addition, increasing the photoperiod from 12 to 16 h accelerated flowering by 17–21 days under a PPFD ≥ 300 µmol∙m−2∙s−1 and first fruit harvest by 17 days at a PPFD of 450 µmol∙m−2∙s−1. Regardless of PPFD, strawberry fruit production (g·m−2·month−1) increased by 372–989% under a 16-h photoperiod in comparison to under a 12-h photoperiod. In contrast, there was little effect of PPFD on fruit production. Our results suggest that increasing the PPFD or photoperiod can increase strawberry plant growth, but increasing the photoperiod can have a dominant effect on increasing early fruit production in strawberry ‘Albion’.
Comparative Transcriptomic Analysis Reveals the Negative Response Mechanism of Peanut Root Morphology and Nitrate Assimilation to Nitrogen Deficiency
Authors: Li Lijie , Cheng Xiangguo , Kong Xiangjun , Jia Peipei , Wang Xiaohui , Zhang Lei , Zhang Xiaotian , Zhang Yi , Zhang Zhiyong , Zhang Baohong
Published: February 2023

Root architecture plays a fundamental role in crop yield, which is sensitive to nitrogen fertilizer. Although it is well studied that nitrogen fertilizer significantly promotes peanut (Arachis hypogaea L.) growth and yield, less information was available on how its root development responds to nitrogen deficiency. In this study, the growth and development of roots were inhibited, as indicated by the significantly decreased root dry weight and length and the lateral root number, especially under 10 days of nitrogen deficiency treatment. The activities and the expression of the genes related to nitrogen assimilation enzymes including nitrate reductase, glutamine synthetase, glutamate dehydrogenase, and glutamine oxoglutarate aminotransferase and the genes encoding the nitrate transporters were significantly decreased under 10 days of nitrogen deficiency treatment, which may lead to a decrease in nitrate content, as indicated by the significantly decreased nitrogen balance index. Transcriptome sequencing revealed a total of 293 (119 up- and 174 downregulated) and 2271 (1165 up- and 1106 downregulated) differentially expressed genes (DEGs) identified after five and ten days of nitrogen deficiency treatments, respectively. Bioinformatic analysis showed that these DEGs were mainly involved in nitrate transportation and assimilation, phytohormone signal transduction, and the lignin biosynthesis pathway. Furthermore, a putative schematic diagram of nitrogen deficiency inhibiting root growth was established, which gives us a better understanding of nitrogen metabolism in peanut roots and a theoretical basis for improving nitrogen use efficiency.
Genome-Wide Association Study of Agronomic and Physiological Traits Related to Drought Tolerance in Potato
Authors: Alvarez-Morezuelas Alba , Barandalla Leire , Ritter Enrique , Ruiz de Galarreta Jose Ignacio
Published: February 2023

Potato (Solanum tuberosum L.) is often considered a water-sensitive crop and its production can be threatened by drought events, making water stress tolerance a trait of increasing interest. In this study, a panel of 144 tetraploid potato genotypes was evaluated for two consecutive years (2019 and 2020) to observe the variation of several physiological traits such as chlorophyll content and fluorescence, stomatal conductance, NDVI, and leaf area and circumference. In addition, agronomic parameters such as yield, tuber fresh weight, tuber number, starch content, dry matter and reducing sugars were determined. GGP V3 Potato array was used to genotype the population, obtaining a total of 18,259 high-quality SNP markers. Marker-trait association was performed using GWASpoly package in R software and Q + K linear mixed models were considered. This approach allowed us to identify eighteen SNP markers significantly associated with the studied traits in both treatments and years, which were related to genes with known functions. Markers related to chlorophyll content and number of tubers under control and stress conditions, and related to stomatal conductance, NDVI, yield and reducing sugar content under water stress, were identified. Although these markers were distributed throughout the genome, the SNPs associated with the traits under control conditions were found mainly on chromosome 11, while under stress conditions they were detected on chromosome 4. These results contribute to the knowledge of the mechanisms of potato tolerance to water stress and are useful for future marker-assisted selection programs.
Experimental Study on Tin Slag Polymer Concrete Strengthening under Compression with Metallic Material Confinement
Authors: Manda Muhamad Soffi , Mat Rejab Mohd Ruzaimi , Hassan Shukur Abu , Wahit Mat Uzir , Nurhadiyanto Didik
Published: February 2023

Studies on the external strengthening of tin slag polymer concrete by fibre-reinforced plastic confinement have provided strength enhancement of tin slag polymer concrete up to 128% with carbon fibre-reinforced plastic confinement. However, the effect of metallic material confinement has yet to be studied. This article presents the experimental finding on tin slag polymer concrete strengthening through metallic material confinement under compressive loads. Machined mild steel metal tube has been employed to strengthen tin slag polymer concrete core in partial and fully confinement prior to compression testing. Through this study, compressive strength of tin slag polymer concrete short column has been enhanced with the metal tube confinement application from 59.19 MPa (unconfined) to 95.86 MPa (partial metal confinement) and 131.84 (full metal confinement) representing 61.95% and 122.74% of strength enhancement percentage. Material behaviour analysis through stress versus strain curves has revealed that the strain softening curve is modified by metal tube confinement before a fracture occurs on both partial and full metal confinement samples compared to the control sample (unconfined). In addition, the failure modes have indicated that the high ductility of metallic confinement material has effectively confined tin slag polymer concrete from sudden fracture where the metal tube in partial confinement indicates ductile expansion while the metal tube in full confinement has shown ductile crushing. In general, it was concluded that metallic material confinement on tin slag polymer concrete under compressive load has resulted in providing strength enhancement and modified the failure mode of tin slag polymer concrete. Finally, further research is recommended, especially by initiating numerical analysis to facilitate parametric studies on tin slag polymer concrete for structural material design.
Impact of Different Ratios of Lignin Waste and Liquid Glass on the Performance Characteristics of Biopolyurethane Foams
Authors: Kairytė Agnė , Šeputytė-Jucikė Jurga , Członka Sylwia , Vėjelis Sigitas , Vaitkus Saulius
Published: February 2023

In the current study, biopolyurethane foam was modified with 2.5–10 wt.% lignin waste (LigW) and liquid glass (LG)-modified LigW particles at different LigW/LG ratios—1:1 and 1:2—and their impact on performance characteristics—i.e., rheology, foaming times, apparent density, thermal conductivity before and after aging, dimensional stability at ambient and elevated conditions, compressive and tensile strengths, short-term water absorption by partial immersion, and water vapor permeability—was determined and evaluated. Structural analysis was implemented and structural parameters were taken into consideration as well. During the study, it was determined that 2.5–10 wt.% particles at the LigW/LG ratio of 1:2 showed a superior impact on the physical and mechanical properties of bioPUR foams. The apparent density only insignificantly increased and was in a density range suitable for commercially available polyurethanes. For particles at 10 wt.% and LigW/LG ratio of 1:1, the thermal conductivity value improved by 3.2%, the compressive strength increased by 153%, while the tensile strength improved by 23.5%, indicating sufficient interfacial adhesion between the filler and polymer matrix. Moreover, the short-term water absorption by partial immersion remained almost unchanged, while the water vapour diffusion resistance factor improved from 43 to 48. Additionally, the incorporation of LigW/LG 1:1 and LigW/LG 1:2 particles made it possible to obtain dimensionally and structurally stable closed-cell bioPUR foams for possible application as thermal insulation in building envelopes.
The Effect of Sub- and Near-Critical Carbon Dioxide Assisted Manufacturing on Medical Thermoplastic Polyurethane
Authors: Baru Sarn-ii , Matthews Siobhan , Marchese Eric , Walsh Philip , Coffey Austin
Published: February 2023

Incorporating thermally labile active pharmaceutical ingredients for manufacturing multifunctional polymeric medical devices is restricted due to their tendency to degrade in the hot melt extrusion process. In this study, the potential of sub- and near-critical carbon dioxide (CO2) as a reversible plasticiser was explored by injecting it into a twin-screw hot melt extrusion process of Pellethane thermoplastic polyurethane to decrease its melt process temperature. Its morphological, throughput, thermal, rheological, and mechanical performances were also evaluated. The resultant extrudates were characterised using scanning electron microscopy, parallel plate rotational rheometer, differential scanning calorimetry, thermogravimetric analysis, and tensile testing. The process temperature decreased from 185 to 160 °C. The rheology indicated that the reduction in melt viscosity was from 690 Pa.s to 439 Pa.s (36%) and 414 Pa.s (40%) at 4.14 and 6.89 MPa, respectively. The tensile modulus in the elastomeric region is enhanced from 5.93 MPa, without CO2 to 7.71 MPa with CO2 at both 4.14 and 6.89 MPa. The results indicate that the employment of both sub- and near-critical CO2 as a processing aid is a viable addition to conventional hot melt extrusion and that they offer more opportunities for thermosensitive drugs to be more stable in the molten stream of Pellethane thermoplastic polyurethane.
Study of Genetic Variation in Bermuda Grass along Longitudinal and Latitudinal Gradients Using Spectral Reflectance
Authors: Zhang Jingxue , Han Mengli , Wang Liwen , Chen Minghui , Chen Chen , Shen Sicong , Liu Jiangui , Zhang Chao , Shang Jiali , Yan Xuebing
Published: February 2023

Genetic variation among populations within plant species can have huge impact on canopy biochemistry and structure across broad spatial scales. Since canopy spectral reflectance is determined largely by canopy biochemistry and structure, spectral reflectance can be used as a means to capture the variability of th genetic characteristics of plant species. In this study, we used spectral measurements of Bermuda grass [Cynodon dactylon (L.) Pers.] at both the leaf and canopy levels to characterize the variability of plant traits pertinent to phylogeographic variation along the longitudinal and latitudinal gradients. An integration of airborne multispectral and hyperspectral data allows for the exploitation of spectral variations to discriminate between the five different genotypic groups using ANOVA and RF models. We evaluated the spectral variability among high-latitude genotypic groups and other groups along the latitudinal gradients and assessed spectral variability along longitudinal gradients. Spectral difference was observed between genetic groups from the northern regions and those from other regions along the latitudinal gradient, which indicated the usefulness of spectral signatures for discriminating between genetic groups. The canopy spectral reflectance was better suited to discriminate between genotypes of Bermuda grass across multiple scales than leaf spectral data, as assessed using random forest models. The use of spectral reflectance, derived from remote sensing, for studying genetic variability across landscapes is becoming an emerging research topic, with the potential to monitor and forecast phenology, evolution and biodiversity.
Travelling Ionospheric Disturbance Direction of Propagation Detection Using Swarm A-C In-Situ Electron Density
Authors: Haralambous Haris , Paul Krishnendu Sekhar
Published: February 2023

We demonstrate a simple method to estimate the direction of propagation of travelling ionospheric disturbances using in-situ electron density measurements in the topside ionosphere by exploiting the side-by-side flying configuration of Swarm A and C satellites. Corresponding cases of TIDs recorded on detrended GPS total electron content (TEC) maps over different continents are used to validate this approach. This simple method could be suitable to detect the direction of propagation of TID wavefronts over the ocean and the polar regions where ground-based GNSS stations are unavailable.
Boba Shop, Coffee Shop, and Urban Vitality and Development—A Spatial Association and Temporal Analysis of Major Cities in China from the Standpoint of Nighttime Light
Authors: Zhou Yuquan , He Xiong , Zikirya Bahram
Published: February 2023

Nighttime light (NTL) is a critical indicator of urban vitality and development. Using NTL as a representation of urban vitality and development, the study explores how different fresh-made beverage shops, namely boba and coffee shops, proxy various facets of urban vitality and development in four megacities in China. Existing studies mostly discuss urban vitality as a broad concept and seldom investigate the diverse urban vitality and development represented by different indicators. This study selects Beijing, Shanghai, Guangzhou, and Shenzhen as case study regions and explores (1) their urban vitality pattern represented by NTL. (2) the heterogeneous spatial distribution of boba and coffee shops; (3) how boba and coffee shops represent urban vitality differently; and (4) how boba and coffee shops portray the economy and population growth aspect of urban development differently. We acquired NTL data from remote sensing images to measure urban vitality and development. Cross-sectionally, the majority of urban vitality and development represented by NTL concentrates in urban centers. The distribution of coffee shops assimilates the spatial pattern of urban vitality represented by NTL while boba shops have a greater spatial extent in metropolitan fringes. Longitudinally, from 2012 to 2020, the global and local bivariate Moran’s I analysis between NTL and beverage shops shows that the coffee shops capture urban vitality and development better than boba shops in Beijing, while the pattern is reversed in Guangzhou and Shenzhen. Examining the evolving spatial dynamics between beverage shops’ growth and urban development using bivariate Moran’s I and Getis–Ord Gi/Mann–Kendall emerging hot spot analysis, we found that the locations with the most intense economic growth have seen the most spatial expansion of coffee shops. In contrast, those with the fastest population growth have seen the greatest spatial development of boba businesses. These results indicate that coffee shops represent the economic aspect of urban vitality while boba shops emphasize the population growth aspects. By examining the dynamic spatio–temporal relationship between small beverage shops and urban vitality and development represented by NTL data, this study broadens the usage of remote sensing data in urban studies and expands on previous research and offers insights for urban planners and geographers to reference when choosing indicators of urban vitality and growth.
Monitoring Land Subsidence along the Subways in Shanghai on the Basis of Time-Series InSAR
Authors: Zhang Jinhua , Ke Changqing , Shen Xiaoyi , Lin Jinxin , Wang Ru
Published: February 2023

In recent years, Shanghai has entered a stage of microscale land subsidence, but the uneven subsidence is still significant, with long-term impacts on the operational safety of subways and other infrastructures. On the basis of 154 high-resolution Terra Synthetic Aperture Radar-X (TerraSAR-X) images captured from 2013 to 2020 and the time-series persistent scatterer-interferometric SAR (PS-InSAR) method, the land subsidence along the subways in Shanghai was acquired, and the levelling data of 56 benchmarks were used to validate the measurements derived by PS-InSAR. The results indicated that the two data sets agreed well, with a correlation coefficient of 0.9 and maximum D-value of 4.0 mm derived from six pairs of comparative data sequences. The proportion of PS points showing deformation rates between −3.0 mm/a and 3.0 mm/a reached 99.4%. These results indicated that the land subsidence trend along the subway was relatively stable overall, while significant deformation was distributed mainly along the suburban subways, especially the lines that were newly open to traffic, such as Line 5 and the Pujiang line (PJ Line); along these lines, the proportions of PS points with deformation rates exceeding ±3 mm/a were 7.2% and 7.6%, respectively, and the proportions were much smaller in the other lines. The maximum cumulative deformation (MCD) along the subways was located between Jiangchuan Road Station and Xidu Station of Line 5 with a value of −66.4 mm, while the second and third MCDs were −48.2 mm along Line 16 and −44.5 mm along PJ Line, respectively. Engineering constructions, such as human-induced ground loads, foundation pit constructions, and road constructions, were the main factors affecting local land subsidence. The analysis results also showed that land subsidence was relatively significant during the period before the subways were open to traffic due to subway construction, while land subsidence clearly slowed after the subway lines were open to traffic. This deceleration in land subsidence was closely related to the rise in the groundwater level.
Baseline Assessment of Ecological Quality Index (EQI) of the Marine Coastal Habitats of Tonga Archipelago: Application for Management of Remote Regions in the Pacific
Authors: Peirano Andrea , Barsanti Mattia , Delbono Ivana , Candigliota Elena , Cocito Silvia , Hokafonu Ta’hirih , Immordino Francesco , Moretti Lorenzo , Matoto Atelaite Lupe
Published: February 2023

The loss of coral habitats and associated biodiversity have direct effects both on the physical dynamics of the coast and on natural resources, threatening the survival of local populations. Conservative actions, such as the creation of new Marine Protected Areas, are urgent measures needed to face climate change. Managers need fast and simple methods to evaluate marine habitats for planning conservation areas. Here, we present the application of an Ecological Quality Index (EQI), developed for regional-scale habitat maps of the Atlas of the Marine Coastal Habitats of the Kingdom of Tonga, by processing Copernicus Sentinel-2 imagery. Both the habitat mapping classification and the EQI application were focused on the importance of coral reef, seagrass and mangrove habitats, both as natural defense and sustenance for the local populations. Twelve main Pacific reef habitats were evaluated through a three-level EQI score assigned to six parameters: nursery ground, connectivity, species reservoir, fish attraction, biodiversity and primary production. The EQI was integrated into a developed georeferenced database associated to the QGIS software providing the ability to identify on the maps the area of interest and the associated habitats, and to quantify their ecological relevance. The EQI is proposed as a tool that can offer to stakeholders and environmental managers a simple and direct indicator of the value of the marine coastal environment. The index may be handled for management purposes of vast areas with remote and uninhabited islands.
From Strain to Loads: Development of a Measurement Solution for Wind Turbine Transmission Input Loads during Drivetrain Testing
Authors: Bilen Eren , Azzam Baher , Schelenz Ralf , Runkel Tim , Raddatz Malte , Jacobs Georg
Published: February 2023

As wind energy is paving the way for the energy transition from fossil to renewable energy sources, the ongoing trend of increasing the rated power of wind turbines aims to reduce the overall cost of wind energy. The resulting increase in drivetrain loads motivates the need for wind turbine (WT) drivetrain testing in the development phase of critical components such as the WT main gearbox (GB). While several WT system test benches allow for the application of emulated rotor loads in six degrees of freedom (6-DOF), the drivetrain input loads can significantly differ from the GB 6-DOF input loads due to the design of the drivetrain under test. However, currently available load measurement solutions are not capable of sensing GB input loads in 6-DOF. Thus, this work aims to develop a methodology for converging signals from a purposely designed sensor setup and turbine specific design parameters to compute the GB 6-DOF input loads during WT testing. Strain gauges (SG) and accelerometers have been installed on the low-speed shaft (LSS) of a WT drivetrain under test at the 4MW WT system test bench at the Center for Wind Power Drives. Using the data of the aforementioned sensors, a methodology for computing the GB input loads is developed. The methodology is validated through comparison to the applied loads data provided by the aforementioned test bench. The results demonstrate the high promise of the proposed method for estimating the GB input loads during WT drivetrain testing.