bluuebunny/crossref_metadata_2025 huggingface dataset:
Immobilization of single argon atoms in nano-cages of two-dimensional zeolite model systems
Authors: Zhong Jian-Qiang , Wang Mengen , Akter Nusnin , Kestell John D. , Boscoboinik Alejandro M. , Kim Taejin , Stacchiola Dario J. , Lu Deyu , Boscoboinik J. Anibal
Published: July 2017

The confinement of noble gases on nanostructured surfaces, in contrast to bulk materials, at non-cryogenic temperatures represents a formidable challenge. In this work, individual Ar atoms are trapped at 300 K in nano-cages consisting of (alumino)silicate hexagonal prisms forming a two-dimensional array on a planar surface. The trapping of Ar atoms is detected in situ using synchrotron-based ambient pressure X-ray photoelectron spectroscopy. The atoms remain in the cages upon heating to 400 K. The trapping and release of Ar is studied combining surface science methods and density functional theory calculations. While the frameworks stay intact with the inclusion of Ar atoms, the permeability of gasses (for example, CO) through them is significantly affected, making these structures also interesting candidates for tunable atomic and molecular sieves. These findings enable the study of individually confined noble gas atoms using surface science methods, opening up new opportunities for fundamental research.
Erratum: Growth of organic crystals via attachment and transformation of nanoscopic precursors
Authors: Jiang Yuan , Kellermeier Matthias , Gebauer Denis , Lu Zihao , Rosenberg Rose , Moise Adrian , Przybylski Michael , Cölfen Helmut
Published: July 2017

Nature Communications 8: Article number: 15933 (2017); Published 21 June 2017; Updated 17 July 2017 The original version of this Article contained an error in the spelling of the author Denis Gebauer, which was incorrectly given as Denis Gebaue. This has now been corrected in both the PDF and HTML versions of the Article.
Tid1-S regulates the mitochondrial localization of EGFR in non-small cell lung carcinoma
Authors: Wang T-H , Lin Y-H , Yang S-C , Chang P-C , Wang T-CV , Chen C-Y
Published: July 2017

The epidermal growth factor receptor (EGFR) is the major driver of non-small cell lung carcinoma (NSCLC). Mitochondrial accumulation of EGFR has been shown to promote metastasis in NSCLC, yet little is known about how the mitochondrial localization of EGFR is regulated. In this work, we show that Tid1 (also known as mitochondrial HSP40) is involved in the mitochondrial localization of EGFR, and that the DnaJ domain of Tid1-S is essential for the Tid1-S-mediated transportation of EGFR into mitochondria. Overexpression of Tid1-S increased the migration and invasion of NSCLC cells cultured in vitro. High levels of EGFR and Tid1-S were detected in the mitochondria of cancerous lesions from stage IV NSCLC patients, and high levels of mitochondrial Tid1-S/EGFR were correlated with lymph node metastasis and poor overall survival of NSCLC patients. We thus conclude that Tid1-S critically governs the mitochondrial localization of EGFR through the mtHSP70 transportation pathway, and that the mitochondrial accumulation of EGFR appears to promote metastasis in NSCLC.
Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm
Authors: Cerrone Marina , Montnach Jerome , Lin Xianming , Zhao Yan-Ting , Zhang Mingliang , Agullo-Pascual Esperanza , Leo-Macias Alejandra , Alvarado Francisco J. , Dolgalev Igor , Karathanos Thomas V. , Malkani Kabir , Van Opbergen Chantal J.M. , van Bavel Joanne J.A. , Yang Hua-Qian , Vasquez Carolina , Tester David , Fowler Steven , Liang Fengxia , Rothenberg Eli , Heguy Adriana , Morley Gregory E. , Coetzee William A. , Trayanova Natalia A. , Ackerman Michael J. , van Veen Toon A.B. , Valdivia Hector H. , Delmar Mario
Published: July 2017

Plakophilin-2 (PKP2) is a component of the desmosome and known for its role in cell–cell adhesion. Mutations in human PKP2 associate with a life-threatening arrhythmogenic cardiomyopathy, often of right ventricular predominance. Here, we use a range of state-of-the-art methods and a cardiomyocyte-specific, tamoxifen-activated, PKP2 knockout mouse to demonstrate that in addition to its role in cell adhesion, PKP2 is necessary to maintain transcription of genes that control intracellular calcium cycling. Lack of PKP2 reduces expression of Ryr2 (coding for Ryanodine Receptor 2), Ank2 (coding for Ankyrin-B), Cacna1c (coding for CaV1.2) and Trdn (coding for triadin), and protein levels of calsequestrin-2 (Casq2). These factors combined lead to disruption of intracellular calcium homeostasis and isoproterenol-induced arrhythmias that are prevented by flecainide treatment. We propose a previously unrecognized arrhythmogenic mechanism related to PKP2 expression and suggest that mutations in PKP2 in humans may cause life-threatening arrhythmias even in the absence of structural disease.
Synthesis of ordered carbonaceous frameworks from organic crystals
Authors: Nishihara Hirotomo , Hirota Tetsuya , Matsuura Kenta , Ohwada Mao , Hoshino Norihisa , Akutagawa Tomoyuki , Higuchi Takeshi , Jinnai Hiroshi , Koseki Yoshitaka , Kasai Hitoshi , Matsuo Yoshiaki , Maruyama Jun , Hayasaka Yuichiro , Konaka Hisashi , Yamada Yasuhiro , Yamaguchi Shingi , Kamiya Kazuhide , Kamimura Takuya , Nobukuni Hirofumi , Tani Fumito
Published: July 2017

Despite recent advances in the carbonization of organic crystalline solids like metal-organic frameworks or supramolecular frameworks, it has been challenging to convert crystalline organic solids into ordered carbonaceous frameworks. Herein, we report a route to attaining such ordered frameworks via the carbonization of an organic crystal of a Ni-containing cyclic porphyrin dimer (Ni2-CPDPy). This dimer comprises two Ni–porphyrins linked by two butadiyne (diacetylene) moieties through phenyl groups. The Ni2-CPDPy crystal is thermally converted into a crystalline covalent-organic framework at 581 K and is further converted into ordered carbonaceous frameworks equipped with electrical conductivity by subsequent carbonization at 873–1073 K. In addition, the porphyrin’s Ni–N4 unit is also well retained and embedded in the final framework. The resulting ordered carbonaceous frameworks exhibit an intermediate structure, between organic-based frameworks and carbon materials, with advantageous electrocatalysis. This principle enables the chemical molecular-level structural design of three-dimensional carbonaceous frameworks.
DNA end resection requires constitutive sumoylation of CtIP by CBX4
Authors: Soria-Bretones Isabel , Cepeda-García Cristina , Checa-Rodriguez Cintia , Heyer Vincent , Reina-San-Martin Bernardo , Soutoglou Evi , Huertas Pablo
Published: July 2017

DNA breaks are complex DNA lesions that can be repaired by two alternative mechanisms: non-homologous end-joining and homologous recombination. The decision between them depends on the activation of the DNA resection machinery, which blocks non-homologous end-joining and stimulates recombination. On the other hand, post-translational modifications play a critical role in DNA repair. We have found that the SUMO E3 ligase CBX4 controls resection through the key factor CtIP. Indeed, CBX4 depletion impairs CtIP constitutive sumoylation and DNA end processing. Importantly, mutating lysine 896 in CtIP recapitulates the CBX4-depletion phenotype, blocks homologous recombination and increases genomic instability. Artificial fusion of CtIP and SUMO suppresses the effects of both the non-sumoylatable CtIP mutant and CBX4 depletion. Mechanistically, CtIP sumoylation is essential for its recruitment to damaged DNA. In summary, sumoylation of CtIP at lysine 896 defines a subpopulation of the protein that is involved in DNA resection and recombination.
Robust hierarchical 3D carbon foam electrode for efficient water electrolysis
Authors: Pham Tung Ngoc , Sharifi Tiva , Sandström Robin , Siljebo William , Shchukarev Andrey , Kordas Krisztian , Wågberg Thomas , Mikkola Jyri-Pekka
Published: July 2017

Herein we report a 3D heterostructure comprising a hierarchical macroporous carbon foam that incorporates mesoporous carbon nanotubes decorated with cobalt oxide nanoparticles as an unique and highly efficient electrode material for the oxygen evolution reaction (OER) in electrocatalytic water splitting. The best performing electrode material showed high stability after 10 h, at constant potential of 1.7 V vs. RHE (reversible hydrogen electrode) in a 0.1 M KOH solution and high electrocatalytic activity in OER with low overpotential (0.38 V vs RHE at 10 mA cm−2). The excellent electrocatalytic performance of the electrode is rationalized by the overall 3D macroporous structure and with the firmly integrated CNTs directly grown on the foam, resulting in a large specific surface area, good electrical conductivity, as well as an efficient electrolyte transport into the whole electrode matrix concurrent with an ability to quickly dispose oxygen bubbles into the electrolyte. The eminent properties of the three-dimensional structured carbon matrix, which can be synthesized through a simple, scalable and cost effective pyrolysis process show that it has potential to be implemented in large-scale water electrolysis systems.
How cholesteryl ester transfer protein can also be a potential triglyceride transporter
Authors: Chirasani Venkat R. , Senapati Sanjib
Published: July 2017

CETP transfers cholesteryl esters (CEs) and triglycerides (TGs) between different lipoproteins and came in limelight as a drug-target against CVD. In the search for detailed mechanism of lipid transfer through CETP, enormous effort is devoted employing crystallographic, cryo-EM, and Molecular Dynamics (MD) studies. However, these studies primarily focused on CE-bound CETP structure and CE transfer mechanism. With the reported correlation that CETP looses significant CE transfer activity upon inhibiting TG transfer, it is of tremendous importance to understand the structure and dynamics of TG-bound CETP. Our results from large-scale all-atom and coarse-grained MD simulations show that CETP can accommodate two TG molecules in parallel N-N orientation with TG oleate chains majorly attaining the tuning-fork conformation. In TG-bound form, CETP not only maintained its secondary structures but also exhibited similar bending-twisting motions as reported for CE-CETP crystal structure. Obtained structural information are further validated by correlating to available functional data of 2–8 fold slower transfer rate of TG through CETP, where we show that TGs make 20% additional contacts with CETP compared to CEs. Identified CETP residues facilitating TG binding also match very well with reported mutagenesis data. The study could accelerate the drug-designing processes to combat CETP functionality and CVD.
Improved Liver R2* Mapping by Averaging Decay Curves
Authors: Zhang Xinyuan , Peng Jie , Wang Changqing , Feng Yanqiu , Feng Qianjin , Li Xinzhong , Chen Wufan , He Taigang
Published: July 2017

Liver R2* mapping is often degraded by the low signal-to-noise ratio (SNR) especially in the presence of severe iron. This study aims to improve liver R2* mapping at low SNRs by averaging decay curves before the process of curve-fitting. Independently filtering echo images by nonlocal means (NLM) demonstrated improved quality of R2* mapping, but may introduce new errors due to the nonlinear nature of the NLM filter, during which the averaging weights may vary with different image contents at multiple echo times. In addition, the image denoising effect of the NLM may decline when no sufficient similar patches are available. To overcome these drawbacks, we proposed to filter decay curves instead of images. In this novel scheme, decay curves were averaged in a local window, each with a weight assigned according to the curve-similarity measured by the distance between one of the neighboring curves and the targeted one. The proposed method was tested on simulated, phantom and patient data. The results demonstrate that the proposed method can provide more accurate R2* mapping compared with the NLM algorithm, and hence has the potential to improve diagnosis and therapy in patients with liver iron.
Thickness dependence of anomalous Nernst coefficient and longitudinal spin Seebeck effect in ferromagnetic NixFe100−x films
Authors: Kannan Harsha , Fan Xin , Celik Halise , Han Xiufeng , Xiao John Q.
Published: July 2017

Spin Seebeck effect (SSE) measured for metallic ferromagnetic thin films in commonly used longitudinal configuration contains the contribution from anomalous Nernst effect (ANE). The ANE is considered to arise from the bulk of the ferromagnet (FM) and the proximity-induced FM boundary layer. We fabricate a FM alloy with zero Nernst coefficient to mitigate the ANE contamination of SSE and insert a thin layer of Cu to separate the heavy metal (HM) from the FM to avoid the proximity contribution. These modifications to the experiment should permit complete isolation of SSE from ANE in the longitudinal configuration. However, further thickness dependence studies and careful analysis of the results revealed, ANE contribution of the isolated FM alloy is twofold, surface and bulk. Both surface and bulk contributions, whose magnitudes are comparable to that of the SSE, can be modified by the neighboring layer. Hence surface contribution to the ANE in FM metals is an important effect that needs to be considered.
Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix
Authors: Bodenberger Nicholas , Kubiczek Dennis , Trösch Laura , Gawanbacht Ali , Wilhelm Susanne , Tielker Denis , Rosenau Frank
Published: July 2017

3D cell culture is a helpful approach to study cell-cell interaction in a native-like environment, but is often limited due the challenge of retrieving cells from the material. In this study, we present the use of recombinant lectin B, a sugar-binding protein with four binding cavities, to enable reversible cell integration into a macroporous protein hydrogel matrix. By functionalizing hydrogel precursors with saccharose, lectin B can both bind to sugar moieties on the cellular surface as well as to the modified hydrogel network. Confocal microscopy and flow cytometry analysis revealed cells to be integrated into the network and to adhere and proliferate. Furthermore, the specificity and reversibility was investigated by using a recombinantly produced yellow fluorescent - lectin B fusion protein and a variety of sugars with diverging affinities for lectin B at different concentrations and elution times. Cells could be eluted within minutes by addition of L-fucose to the cell-loaded hydrogels to make cells available for further analysis.
Role of HDAC9-FoxO1 Axis in the Transcriptional Program Associated with Hepatic Gluconeogenesis
Authors: Chen Jizheng , Zhang Zhilei , Wang Ning , Guo Min , Chi Xiumei , Pan Yu , Jiang Jing , Niu Junqi , Ksimu Sulaiman , Li John Zhong , Chen Xinwen , Wang Qian
Published: July 2017

Histone deacetylase 9 (HDAC9) regulates hepatic gluconeogenesis by deacetylating Forkhead box O 1 (FoxO1). HDAC9 upregulation is involved in hepatitis C virus (HCV)-associated exaggerated gluconeogenesis. Herein, we found in addition to FoxO1, HDAC9 also regulates other gluconeogenic transcription factors, including peroxisomeproliferator-activated receptor-γ coactivator-1α (PGC-1α), cyclic AMP-responsive element-binding protein (CREB), and glucocorticoid receptor (GR). Unlike FoxO1, which is regulated by post-translational modification responses to HDAC9, HDAC9 regulates PGC-1α, CREB and GR by altering gene expression. Similar to PGC-1α, CREB and GR were found to be novel regulatory targets of FoxO1 by examination of the FoxO1 binding site in their promoter. PGC-1α, CREB and GR were upregulated in response to HDAC9 via FoxO1 deacetylation. These findings indicate that HDAC9-FoxO1 signalling contributes to gluconeogenesis by modulating the expression of gluconeogenic transcription factors. In particular, metabolic profiling demonstrated a clear shift towards gluconeogenesis metabolism, and HDAC9-FoxO1 signalling can be strongly induced to upregulate gluconeogenic transcription factors following HCV infection. The positive correlation between HDAC9 and gluconeogenic transcription factor expression levels in the livers of both HCV-infected patients and normal individuals further emphasizes the clinical relevance of these results. Thus, HDAC9-FoxO1 signalling axis is involved in regulating gluconeogenic transcription factors, gluconeogenesis, and HCV-induced type 2 diabetes.
Partitioning Evapotranspiration into Green and Blue Water Sources in the Conterminous United States
Authors: Velpuri Naga Manohar , Senay Gabriel B.
Published: July 2017

In this study, we combined two 1 km actual evapotranspiration datasets (ET), one obtained from a root zone water balance model and another from an energy balance model, to partition annual ET into green (rainfall-based) and blue (surface water/groundwater) sources. Time series maps of green water ET (GWET) and blue water ET (BWET) are produced for the conterminous United States (CONUS) over 2001–2015. Our results indicate that average green and blue water for all land cover types in CONUS accounts for nearly 70% and 30% of the total ET, respectively. The ET in the eastern US arises mostly from GWET, and in the western US, it is mostly BWET. Analysis of the BWET in the 16 irrigated areas in CONUS revealed interesting results. While the magnitude of the BWET gradually showed a decline from west to east, the increase in coefficient of variation from west to east confirmed greater use of supplemental irrigation in the central and eastern US. We also established relationships between different hydro-climatology zones and their blue water requirements. This study provides insights on the relative contributions and the spatiotemporal dynamics of GWET and BWET, which could lead to improved water resources management.
CID fragmentation, H/D exchange and supermetallization of Barnase-Barstar complex
Authors: Kostyukevich Yury , Shulga Aleksej A. , Kononikhin Alexey , Popov Igor , Nikolaev Eugene , Deyev Sergey
Published: July 2017

The barnase-barstar complex is one of the most stable protein-protein complexes and has a very wide range of possible applications. Here we report the use of top-down mass spectrometry for the investigation of the structure of this complex, its ionization via ESI, isolation and fragmentation. It was found that the asymmetry of the resulting charge state distributions of the protein monomer product ions increased as the charge state of the precursor ions increased. For the investigation of the 3D structure of the complex, the gas phase H/D exchange reaction was used. In addition, supermetallized ions of the complex with Zn were produced and investigated. It was observed that an increase in the number of metals bound to the complex results in a change in complex stability and the charge distribution between protein fragment. Analysis of the fragmentation pattern of the supermetallized complex [bn-b* + 5Zn]10+ indicated that this ion is present in different conformations with different charges and Zn distributions. Since Zn cannot migrate, such structures must be formed during ionization.
GeneXpert MTB/RIF assay in the diagnosis of urinary tuberculosis from urine specimens
Authors: Pang Yu , Shang Yuanyuan , Lu Jie , Liang Qian , Dong Lingling , Li Yunxu , Zhao Liping , Jiang Guanglu , Huang Hairong
Published: July 2017

Conventional bacteriological methods are not generally helpful in diagnosing urinary tuberculosis (UTB). GeneXpert is endorsed for the detection of pulmonary tuberculosis, whereas the data on its utility for urine specimens is limited. In this study, we aimed to evaluate its performance on urine specimens in a country with high TB incidence. A total of 163 suspected UTB patients were consecutively enrolled in the analysis, including 37 (22.7%) culture-positive and 44 (27.0%) clinically diagnosed UTB cases. Compared with conventional culture, the sensitivity of GeneXpert (94.6%) was significantly higher than that of smear microscopy (40.5%, P < 0.001). When setting clinical diagnosis as gold standard, 51 out of 81 clinically diagnosed UTB cases were detected by GeneXpert, demonstrating a sensitivity of 63.0%, which was significantly higher than that of smear microscopy (18.5%, P < 0.001) and culture (45.7%, P = 0.027), respectively. In addition, the proportion of UTB cases in the migrant population was significantly higher than that in the resident population (P = 0.019). To conclude, our data demonstrate that GeneXpert outperforms AFB smear and culture for the detection of MTB in urine samples, which provides an alternative for the diagnosis of UTB. The migrant population and previously diagnosed TB cases are high risk factors for developing UTB cases.
Efficacy of Cyclophosphamide treatment for immunoglobulin G4-related disease with addition of glucocorticoids
Authors: Yunyun Fei , Yu Chen , Panpan Zhang , Hua Chen , Di Wu , Lidan Zhao , Linyi Peng , Li Wang , Qingjun Wu , Xuan Zhang , Yan Zhao , Xiaofeng Zeng , Fengchun Zhang , Wen Zhang
Published: July 2017

Aim to evaluate the efficacy and safety of glucocorticoid monotherapy vs combination therapy of cyclophosphamide (CYC) for IgG4 related disease (IgG4-RD). 102 newly diagnosed IgG4-RD patients were enrolled and assigned to 2 groups: Group I was prednisone monotherapy (0.5–1.0 mg/kg.d, tapered gradually) and Group II was glucocorticoid and CYC (50–100 mg per day). Patients were assessed at different periods. Primary end point was relapse rate; secondary end points included response, remission rate and adverse effects. 52 patients were in Group I and 50 in Group II. At 1 month, both groups achieved obvious improvement. Accumulated relapse rate during 1 year was 38.5% in Group 1, including 12 cases with clinical relapse and 8 patients manifesting only serological relapse; whereas there was 12.0% of relapse in Group 2, only 1 with clinical relapse and other 5 patients got serological relapse. The mean flare time in Group II was significantly longer than that in Group I. All relapsing patients in Group I were sensitive to immunosuppressants. Most patients involving more than 6 organs in Group I relapsed during 1 year. IgG4 levels of relapse cases were significantly higher than non-relapsing patients at baseline. Bile duct, lacrimal glands and lymph nodes were commonly relapsed organs in Group I.
Photoluminescence associated with the site occupations of Ho3+ ions in BaTiO3
Authors: Lu Da-Yong , Guan Dong-Xue
Published: July 2017

A nominal (Ba1−x
Ho
x
)Ti1−x/4O3 (x = 0.01) (BHTH) ceramic with a single-phase tetragonal structure was prepared at 1400 °C using the solid-state reaction method. The analysis on the defect chemistry revealed that the real formula of BHTH is (Ba1−x
Ho3x/4)(Ti1−x/4Ho
x/4)O3 with Ba vacancies via electron paramagnetic resonance (EPR). Photoluminescence (PL) was investigated on the basis of excitation with different wavelength lasers. The results indicated that under 488-nm excitation, PL and Raman scattering can occur simultaneously as two distinct optical processes for BHTH ceramic powders, and the strongest PL band at 564 nm was discovered and verified to originate from the 5G6/5F1 → 5I7 transition of Ho3+ ions on the Ti sites in the BaTiO3 lattice. Upon 532- and 638-nm excitations, three PL bands of 5F4/5S2 → 5I8, 5F5 → 5I8, and 5F4/5S2 → 5I7 transitions are attributed to the contributions from Ho3+ ions on the Ba sites. The common Raman spectrum of BaTiO3 can be observed without PL disturbance using 785-nm excitation wavelength. The PL effect may provide a probe for the site occupations of Ho3+ ions in widely-used BaTiO3 dielectric ceramics co-doped with Ho3+ and other dopants.
Trainee Surgeons Affect Operative Time but not Outcome in Minimally Invasive Total Hip Arthroplasty
Authors: Weber Markus , Benditz Achim , Woerner Michael , Weber Daniela , Grifka Joachim , Renkawitz Tobias
Published: July 2017

Training of young surgeons in total hip arthroplasty (THA) is crucial, but might affect operative time and outcome especially in minimally invasive (MIS) THA. We asked whether the learning curve of orthopaedic residents trained on MIS THA has an impact on (1) operative time (2) complication rates and (3) early postoperative outcome. In a retrospective analysis of over 1000 MIS THAs from our institutional joint registry, operative time, complication rates, patient reported outcome measures (Western Ontario and McMaster Universities Arthritis Index [WOMAC] and Euro-Qol 5D-5L [EQ-5D]) within the first year and responder rates for positive outcome as defined by the Outcome Measures in Rheumatology and Osteoarthritis Research Society International consensus responder (OMERACT-OARSI) criteria were compared between trainee and senior surgeons. Mean operative time was nine minutes longer for trainees compared to senior surgeons (78.1 ± 25.4 min versus 69.3 ± 23.8 min, p < 0.001). Dislocation (p = 0.21), intraoperative fracture (p = 0.84) and infection rates (p = 0.58) were comparably low in both groups. Both trainee and senior THAs showed excellent improvement of EQ-5D (0.34 ± 0.26 versus 0.32 ± 0.23, p = 0.40) and WOMAC (45.9 ± 22.1 versus 44.9 ± 20.0, p = 0.51) within the first year after surgery without clinical relevant differences. Similarly, responder rates for positive outcome were comparable between trainees with 92.9% and senior surgeons with 95.2% (p = 0.17). MIS THA seems to be a safe procedure during the learning curve of young orthopaedic specialists, but requires higher operative time.
Improving nitrogen source utilization from defatted soybean meal for nisin production by enhancing proteolytic function of Lactococcus lactis F44
Authors: Liu Jiaheng , Zhou Jianjian , Wang Lihong , Ma Zelin , Zhao Guangrong , Ge Zhiqiang , Zhu Hongji , Qiao Jianjun
Published: July 2017

Nisin, one kind of natural antimicrobial peptide, is produced by certain Lactococcus lactis strains, which generally require expensive high-quality nitrogen sources due to limited ability of amino acids biosynthesis. Here we use defatted soybean meal (DSM) as sole nitrogen source to support L. lactis growth and nisin production. DSM medium composition and fermentation conditions were optimized using the methods of Plackett-Burman design and central composite design. The highest nisin production of 3879.58 IU/ml was obtained in DSM medium, which was 21.3% higher than that of commercial medium. To further increase the utilization ability of nitrogen sources, we enhanced the proteolytic function in L. lactis through rationally expressing the related enzymes, which were selected according to the compositions of amino acids and molecular weight of peptides in DSM medium. Significantly, an artificial proteolytic system consisting of a heterologous protease (NprB), an oligopeptides transporter subunit (OppA) and two peptidases (PepF and PepM) was introduced into L.lactis. The constructed strain BAFM was capable of achieving efficient biomass accumulation and nisin yield with 30% decreased amount of DSM hydrolysates, which further reduced the cost of nisin production. The strategy described here offers opportunities for low-cost L. lactis fermentation and large-scale nisin production in industry.
Semi-quantitative analysis of multiple chemical mixtures in solution at trace level by surface-enhanced Raman Scattering
Authors: Zou Sumeng , Hou Mengjing , Li Jianghao , Ma Lingwei , Zhang Zhengjun
Published: July 2017

Surface-enhanced Raman scattering (SERS) technology combines with chemometric method of principal component analysis (PCA) was used to calculate the composition of chemical mixtures in solution. We reported here that there exists composition discrepancy between molecules in solution and molecules adsorbed on Ag@Al2O3 nanorods substrates due to difference in adsorption kinetics of each component. We proposed here a way to calculate the adsorption kinetics factor for each component using a standard sample as the reference, with which one could correct the predictions given by PCA. We demonstrate the validity of this approach in estimating the compositions of mixtures with two, three and four components of 1, 4-Benzenedithiol, 2-Naphthalenethiol, 4-Mercaptobenzoic acid, and 4-Mercaptopyridine molecules, with acceptable errors. Furthermore, a general formula applied to more complex mixtures was proposed to calculate compositions in solution.
Synthesis of renewable high-density fuel with isophorone
Authors: Wang Wei , Liu Yanting , Li Ning , Li Guangyi , Wang Wentao , Wang Aiqin , Wang Xiaodong , Zhang Tao
Published: July 2017

1,1,3-Trimethyl-5-(2,4,4-trimethylcyclohexyl)cyclohexane, a renewable high density fuel, was first produced in a high overall carbon yield (~70%) with isophorone which can be derived from hemicellulose. The synthetic route used this work contains three steps. In the first step, 3,3,5-trimethylcyclohexanone was synthesized by the selective hydrogenation of isophorone. Among the investigated catalysts, the Pd/C exhibited the highest activity and selectivity. Over this catalyst, a high carbon yield (99.0%) of 3,3,5-trimethylcyclohexanone was achieved under mild conditions (298 K, 2 MPa H2, 1 h). In the second step, 3,5,5-trimethyl-2-(3,3,5-trimethylcyclohexylidene)cyclohexanone was produced in a high carbon yield (76.4%) by the NaOH catalyzed self-aldol condensation of 3,3,5-trimethylcyclohexanone which was carried out in a round bottom flask attached to the Dean–Stark apparatus. In the third step, the 3,5,5-trimethyl-2-(3,3,5-trimethylcyclohexylidene)cyclohexanone was hydrodeoxygenated under solvent-free conditions. High carbon yield (93.4%) of 1,1,3-trimethyl-5-(2,4,4-trimethylcyclohexyl)cyclohexane was obtained over the Ni/SiO2 catalyst. The 1,1,3-trimethyl-5-(2,4,4-trimethylcyclohexyl)cyclohexane as obtained has a density of 0.858 g mL−1 and a freezing point of 222.2 K. As a potential application, it can be blended into conventional fuels (such as RP-1, RG-1, etc.) for rocket propulsion.
Two-Year Follow-Up of Vascular Events in Peripheral Arterial Disease Treated With Antiplatelet Agents: A Prospective Observational Multicenter Cohort Study (SEASON)
Authors: Higashi Yukihito , Miyata Tetsuro , Shigematsu Hiroshi , Origasa Hideki , Fujita Masatoshi , Matsuo Hiroshi , Naritomi Hiroaki , Matsuda Hiroaki , Nakajima Masahide , Awano Hideto
Published: July 2017

The present analysis was intended to evaluate the real-world management of peripheral arterial disease (PAD) in Asia, and to explore cardiovascular events in patients with PAD undergoing antiplatelet therapy over 2 years of follow-up. The Surveillance of cardiovascular Events in Antiplatelet-treated arteriosclerosis Obliterans patients in JapaN (SEASON) registry is a prospective observational multicenter study of cardiovascular events in antiplatelet-treated patients with PAD in Japan. The SEASON registry included 11,375 patients who were scheduled to receive treatment for PAD. Two analysis populations were defined: a real-world population (RWP; n = 10,322) and a definite PAD population (DPP; n = 3992) who had ankle-brachial pressure index (ABPI) <0.9 and intermittent claudication, or a history of lower limb revascularization. The primary outcome measure was the rate of the composite of cerebrovascular, cardiovascular, and peripheral vascular events. The composite event rates (95% confidence interval) were 3.28 (3.00–3.57) and 5.71 (5.13–6.34) events per 100 patient-years in the RWP and DPP groups, respectively. Fontaine IV classification and ABPI <0.4 at baseline were both identified as strong risk factors for vascular events. These findings contribute to understanding the situation for real-world patients with PAD receiving antiplatelet therapy.
Correction: Corrigendum: A UK wide cohort study describing management and outcomes for infants with surgical Necrotising Enterocolitis
Authors: Allin Benjamin , Long Anna-May , Gupta Amit , Knight Marian , Lakhoo Kokila ,   British Association of Paediatric Surgeons Congenital Anomalies Surveillance System Necrotising Enterocolitis Collaboration, Kazmierski Marcin , Kenny Simon , Lopes Joana , Cusick Eleri , Parsons Gilian , McCabe Amanda , Upadhyaya Manasvi , Walker Gregor , De Coppi Paulo , Besarovic Sania , Thakkar Hemanshoo , Tullie Lucinda , Sutcliffe Jonathan , Eradi Bala , Ross Andrew , Maphango Nomsa , Motiwale Sandeep , Salloum Adnan , Pardy Caroline , Waly Ramy , Charlesworth Paul , Craigie Ross , Lall Anupam , Lindley Richard , Johal Navroop , Njere Ike , Mortell Alan , Nandi Bip , Jones Abigail , Fouad Dina , tan Yew-Wei , Kufeji Dorothy , Stanwell Joanna , Lakshminarayanan Bhanu , Burge David , Wetherill Charlotte , Niyogi Anindya , Parsons Chris , Doyle Miriam , Turner Alex , Yardley Ian , Shrestha Ram , Mullassery Dhanya , Paramalingham Saravankumar , Ragazzi Simone
Published: July 2017

Scientific Reports 7: Article number: 41149; published online: 27 January 2017; updated: 17 July 2017. The Acknowledgements section in this Article is incomplete. “This article is submitted as an observational study in the category of original articles. A small sub-set of information from this article has been presented at the annual congress of the British Association of Paediatric Surgeons”.
Proton-propelled methyl-tert-butyl ether extraction for highly efficient mass spectrometric analysis of phospholipids in human plasma
Authors: Zhou Qiuhong , Ye Xiaoxue , Nie Bei
Published: July 2017

Proton-propelled selective phospholipid extraction was found to be a clean and effective technique for mass spectrometric analysis, paving a new avenue for multidimensional separation and isolation with considerable resolution.
An electrochemical aptasensor for detection of bovine interferon gamma
Authors: Crulhas Bruno P. , Hadley Dustin , Liu Ying , Shin Dong-Sik , Stybayeva Gulnaz , Imanbekova Meruyert , Hill Ashley E. , Pedrosa Valber , Revzin Alexander
Published: July 2017

Bovine tuberculosis (TB) is an infectious disease caused byMycobacterium tuberculosis(MTB) in which it is hard to identify the pathological symptoms.
Optimizing sampling strategies for NMR-based metabolomics of human feces: pooled vs. unpooled analyses
Authors: Lamichhane Santosh , Sundekilde Ulrik Kræmer , Blædel Trine , Dalsgaard Trine K. , Larsen Lesli H. , Dragsted Lars O. , Astrup Arne , Bertram Hanne C.
Published: July 2017

This study focused on elucidating fecal sample collection for 1H NMR-based metabolite profiling.
Prediction of fatty methyl esters and physical properties of soybean oil/biodiesel blends from near and mid-infrared spectra using the data fusion strategy
Authors: Luna Aderval S. , Lima Igor C. A. , Henriques Cristiane A. , de Araujo Lucia R. R. , Fortunato da Rocha Werickson , da Silva Juliana V.
Published: July 2017

The data fusion was compared to the one sensor methodology to evaluate chemical and physical properties of soybean oil/biodiesel blends.
An amorphous FeMoS4 nanorod array toward efficient hydrogen evolution electrocatalysis under neutral conditions
Authors: Ren Xiang , Wang Weiyi , Ge Ruixiang , Hao Shuai , Qu Fengli , Du Gu , Asiri Abdullah M. , Wei Qin , Chen Liang , Sun Xuping
Published: July 2017

As a durable catalyst, an FeMoS4 nanorod array on carbon cloth shows high activity for hydrogen evolution in neutral media, achieving a geometrical catalytic current density of 10 mA cm−2 at an overpotential of 204 mV.
Surface-initiated synthesis of bulk-imprinted magnetic polymers for protein recognition
Authors: Boitard Charlotte , Rollet Anne-Laure , Ménager Christine , Griffete Nébéwia
Published: July 2017

We developed a novel pathway to synthesize protein imprinted polymer coatings on magnetic nanoparticles in water at room temperature.
A novel enzymatic method for synthesis of glycopeptides carrying natural eukaryotic N-glycans
Authors: Xu Yangyang , Wu Zhigang , Zhang Peiru , Zhu He , Zhu Hailiang , Song Qitao , Wang Li , Wang Faxing , Wang Peng George , Cheng Jiansong
Published: July 2017

Production of glycopeptides carrying complex-type N-glycans via three steps of enzymatic reactions.
Pushing the limits of automated glycan assembly: synthesis of a 50mer polymannoside
Authors: Naresh K. , Schumacher F. , Hahm H. S. , Seeberger P. H.
Published: July 2017

A 102 step automated synthesis produces a 50mer glycan and provides a basis for investigations into carbohydrate materials.
A DNA nanoribbon as a potent inhibitor of metallo-β-lactamases
Authors: Ouyang Xiangyuan , Chang Ya-Nan , Yang Ke-Wu , Wang Wen-Ming , Bai Jian-Jian , Wang Jian-Wei , Zhang Yue-Juan , Wang Si-Yao , Xie Bin-Bin , Wang Li-Li
Published: July 2017

A DNA nanoribbon has been discovered that inhibits the activity of metallo-β-lactamases by binding to the enzyme.
Visible light sensitization of benzoyl azides: cascade cyclization toward oxindoles via a non-nitrene pathway
Authors: Bagal Dattatraya B. , Park Sung-Woo , Song Hyun-Ji , Chang Sukbok
Published: July 2017

Visible light photocatalytic activation of benzoyl azides is implemented for a cascade cyclization toward synthetically versatile oxindoles and spirooxindoles.
Using anion recognition to control the folding and unfolding of a single chain phosphorescent polymer
Authors: Ji Xiaofan , Guo Chenxing , Ke Xian-Sheng , Chi Xiaodong , Sessler Jonathan L.
Published: July 2017

Calix[4]pyrrole-based anion recognition allows control over the folding and unfolding of a single PMMA polymeric chain bearing pendant calix[4]pyrrole and Pt(ii) porphyrin subunits.
Intense redox-driven chiroptical switching with a 580 mV hysteresis actuated through reversible dimerization of an azoniahelicene
Authors: Brandt Jochen R. , Pospíšil Lubomír , Bednárová Lucie , da Costa Rosenildo Correa , White Andrew J. P. , Mori Tadashi , Teplý Filip , Fuchter Matthew J.
Published: July 2017

Electrochemically-reversible dimerisation of an azoniahelicene provides a chiroptical switching system that exhibits significant hysteresis and thereby a large area of bistability.
Post-synthetic transformation of a Zn(ii) polyhedral coordination network into a new supramolecular isomer of HKUST-1
Authors: Chen Yao , Wojtas Lukasz , Ma Shengqian , Zaworotko Michael J. , Zhang Zhenjie
Published: July 2017

A Zn-based porphyrin containing metal–organic material (porphMOM-1) was post-synthetically transformed into a Cu-based porphyrin-encapsulating metal–organic material (porph@HKUST-1-β). HKUST-1-β is a new supramolecular isomer of HKUST-1.
Nonhelical heterometallic [Mo2M(npo)4(NCS)2] string complexes (M = Fe, Co, Ni) with high single-molecule conductance
Authors: Chang Wei-Cheng , Chang Che-Wei , Sigrist Marc , Hua Shao-An , Liu Tsai-Jung , Lee Gene-Hsiang , Jin Bih-Yaw , Chen Chun-hsien , Peng Shie-Ming
Published: July 2017

Nonhelical metal strings, [MoMoM(npo)4(NCS)2], exhibited better overlaps of dx2−y2 between neighbouring metal centres, stronger MoMo bonds, and superior single-molecule conductance.
Developing force fields when experimental data is sparse: AMBER/GAFF-compatible parameters for inorganic and alkyl oxoanions
Authors: Kashefolgheta Sadra , Vila Verde Ana
Published: July 2017

A hybrid approach using ab initio simulations and experimental data yielding reliable, internally consistent parameters for ion–water and anion–cation interactions.
Theoretical tuning of the singlet–triplet energy gap to achieve efficient long-wavelength thermally activated delayed fluorescence emitters: the impact of substituents
Authors: Wang Lijuan , Li Tao , Feng Peicheng , Song Yan
Published: July 2017

Long-wavelength TADF emitters could be achieved with both small ΔEST and high kr through introducing small electron-withdrawing substituents!
Styryl dye formation promoted by catalytic centers of piperazine bound to a silica surface traced by single molecule fluorescence microscopy
Authors: Lino Aline M. , Gehlen Marcelo H.
Published: July 2017

Knoevenagel condensation producing a fluorescent dye is studied at a single molecule level establishing a new method to investigate the molecular rate of amino catalysis.
Multiscale molecular simulations on interfacial adsorption and permeation of nanoporous graphynes
Authors: Yang Jie , Xu Zhijun , Yang Xiaoning
Published: July 2017

Unique interfacial adsorption and permeation on nanoporous graphynes have been revealed by a multiscale simulation strategy.
The molecular and supramolecular aspects in mononuclear manganese(iii) Schiff-base spin crossover complexes
Authors: Wang Shi , Li Ya-Juan , Ju Fei-Fei , Xu Wu-Tan , Kagesawa Koichi , Li Yong-Hua , Yamashita Masahiro , Huang Wei
Published: July 2017

A cooperative SCO process with a hysteresis width of 18 K is the largest one reported in d4 SCO chemistry.
Potassium isopropyl xanthate (PIX): an ultra-efficient palladium scavenger
Authors: Ren Hong , Strulson Christopher A. , Humphrey Guy , Xiang Rong , Li Guangtao , Gauthier Donald R. , Maloney Kevin M.
Published: July 2017

The increasing employment of palladium-catalyzed reactions in the synthesis of active pharmaceutical ingredients (APIs) has created a pressing need for ultra-efficient palladium removal of the resulting metal contaminants.
Synthesis and investigation of well-defined silane terminated and segmented waterborne hybrid polyurethanes
Authors: Zhao Hui , Huang Da , Hao Tong-Hui , Hu Guo-Hua , Ye Gong-bo , Jiang Tao , Zhang Qun-Chao
Published: July 2017

The properties of waterborne polyurethanes were improved significantly by incorporating silane both in the backbone and side chains.
Fabrication of 4-aminophenol sensor based on hydrothermally prepared ZnO/Yb2O3nanosheets
Authors: Rahman Mohammed M. , Alam M. M. , Asiri Abdullah M. , Awual Md Rabiul
Published: July 2017

A facile hydrothermal process was used to prepare nanostructures of ZnO/Yb2O3in alkaline medium, which were applied for efficient chemical sensor development. The sensor fabricated with ZnO/Yb2O3nanostructures may be a promising sensitive chemical sensor for the effective detection of environmental effluents.
Hot plasmonic electron-driven catalytic reactions on patterned metal–insulator–metal nanostructures
Authors: Kim Sun Mi , Lee Changhwan , Goddeti Kalyan C. , Park Jeong Young
Published: July 2017

We fabricated two-dimensional (2D) arrays of metal–insulator–metal (MIM) plasmonic nanoislands designed to efficiently shuttle hot plasmonic electrons. These MIM nanostructures exhibit higher catalytic activity under light irradiation, revealing a significant impact on the catalytic activity for CO oxidation.
Poly(methylhydrosiloxane) as a green reducing agent in organophosphorus-catalysed amide bond formation
Authors: Hamstra Daan F. J. , Lenstra Danny C. , Koenders Tjeu J. , Rutjes Floris P. J. T. , Mecinović Jasmin
Published: July 2017

In situ reduction of phosphine oxide by poly(methylhydrosiloxane) leads to efficient amidation reaction between carboxylic acids and amines.
An anchoring strategy leads to enhanced proton conductivity in a new metal–organic framework
Authors: Lo Tien H. N. , Nguyen My V. , Tu Thach N.
Published: July 2017

An anchoring strategy affords a 900-fold enhancement in the proton conductivity of a new Zr-based metal–organic framework.
Phosphinodifluoroalkylation of alkynes using P(O)H compounds and ethyl difluoroiodoacetate
Authors: Zhang Pengbo , Ying Jianxi , Tang Guo , Zhao Yufen
Published: July 2017

The first phosphinodifluoroalkylation of alkynes is achieved and the simultaneous addition of both difluoromethylene and phosphinoyl groups across the alkynes with high regio- and stereoselectivity is of great significance.
Investigation of high performance TiO2nanorod array perovskite solar cells
Authors: Liu Changwen , Zhu Ruixue , Ng Annie , Ren Zhiwei , Cheung Sin Hang , Du Lili , So Shu Kong , Zapien Juan Antonio , Djurišić Aleksandra B. , Lee Phillips David , Surya Charles
Published: July 2017

Record high and hysteresis free perovskite based solar cells are achieved by crystal engineering and optimization of carrier transport pathway.
Maricite NaFePO4/C/graphene: a novel hybrid cathode for sodium-ion batteries
Authors: Rahman Md Mokhlesur , Sultana Irin , Mateti Srikanth , Liu Junnan , Sharma Neeraj , Chen Ying
Published: July 2017

A maricite hybrid cathode of NaFePO4/C/graphene with a novel microstructure is produced by a modified ball-milling process based on a solid-state reaction. This structure is capable of delivering high sodium storage capacity with outstanding cycle stability.
Structural evolutions and significantly reduced thermal degradation of red-emitting Sr2Si5N8:Eu2+via carbon doping
Authors: Liu Lihong , Wang Le , Li Yuanqiang , Zhang Chenning , Cho Yujin , Or Siu Wing , Zhou Tianliang , Takeda Takashi , Hirosaki Naoto , Xie Rong-Jun
Published: July 2017

Carbon-doping in Sr2Si5N8:Eu2+ results in enhanced reduction of Eu3+ and the covalence of Eu–(N,C) bonds, finally leading to enhanced thermal degradation.
Variable Practice to Enhance Speech Learning in Ultrasound Biofeedback Treatment for Childhood Apraxia of Speech: A Single Case Experimental Study
Authors: Preston Jonathan L. , Leece Megan C. , McNamara Kerry , Maas Edwin
Published: July 2017

Purpose

The purpose of this study was to evaluate the role of practice variability, through prosodic variation during speech sound training, in biofeedback treatment for children with childhood apraxia of speech. It was hypothesized that variable practice would facilitate speech sound learning.

Method

Six children ages 8–16 years with persisting speech sound errors due to childhood apraxia of speech participated in a single-subject experimental design. For each participant, 2 speech sound targets were treated with ultrasound visual feedback training: one with prosodic variation (i.e., practicing sound targets in words and phrases spoken fast, slow, loud, as a question, command, and declarative), and one without prosodic variation. Each target was treated for half of the 1-hr session for 14 treatment sessions.

Results

As measured by standardized effect sizes, all participants showed greater change on generalization probes for sound targets treated under the prosodic variation condition with mean effect sizes (
d
2
) of 14.5 for targets treated with prosodic variation and 8.3 for targets treated without prosodic variation. The average increase in generalization scores was 38% in the prosodic variation condition compared to 31% without.

Conclusions

Ultrasound visual feedback may facilitate speech sound learning and learning may be enhanced by treating speech sounds with explicit prosodic variation.

Supplemental Materials

https://doi.org/10.23641/asha.5150119
Identifying the Dimensionality of Oral Language Skills of Children With Typical Development in Preschool Through Fifth Grade
Authors: Lonigan Christopher J. , Milburn Trelani F.
Published: July 2017

Purpose

Language is a multidimensional construct from prior to the beginning of formal schooling to near the end of elementary school. The primary goals of this study were to identify the dimensionality of language and to determine whether this dimensionality was consistent in children with typical language development from preschool through 5th grade.

Method

In a large sample of 1,895 children, confirmatory factor analysis was conducted with 19–20 measures of language intended to represent 6 factors, including domains of vocabulary and syntax/grammar across modalities of expressive and receptive language, listening comprehension, and vocabulary depth.

Results

A 2-factor model with separate, highly correlated vocabulary and syntax factors provided the best fit to the data, and this model of language dimensionality was consistent from preschool through 5th grade.

Conclusion

This study found that there are fewer dimensions than are often suggested or represented by the myriad subtests in commonly used standardized tests of language. The identified 2-dimensional (vocabulary and syntax) model of language has significant implications for the conceptualization and measurement of the language skills of children in the age range from preschool to 5th grade, including the study of typical and atypical language development, the study of the developmental and educational influences of language, and classification and intervention in clinical practice.

Supplemental Materials

https://doi.org/10.23641/asha.5154220