bluuebunny/crossref_metadata_2025 huggingface dataset:
Activation of A2A Receptor by PDRN Reduces Neuronal Damage and Stimulates WNT/β-CATENIN Driven Neurogenesis in Spinal Cord Injury
Authors: Irrera Natasha , Arcoraci Vincenzo , Mannino Federica , Vermiglio Giovanna , Pallio Giovanni , Minutoli Letteria , Bagnato Gianluca , Anastasi Giuseppe Pio , Mazzon Emanuela , Bramanti Placido , Squadrito Francesco , Altavilla Domenica , Bitto Alessandra
Published: May 2018

Spinal cord injury (SCI) is a complex clinical and progressive condition characterized by neuronal loss, axonal destruction and demyelination. In the last few years, adenosine receptors have been studied as a target for many diseases, including neurodegenerative conditions. The aim of this study was to investigate the effects of an adenosine receptor agonist, PDRN, in an experimental model of SCI. Moreover, since adenosine receptors stimulation may also activate the Wnt pathway, we wanted to study PDRN effects on Wnt signaling following SCI. Spinal trauma was induced by extradural compression of spinal cord at T5-T8 level in C57BL6/J mice. Animals were randomly assigned to the following groups: Sham (n= 10), SCI (n= 14), SCI+PDRN (8 mg/kg/i.p.;n= 14), SCI+PDRN+DMPX (8 and 10 mg/kg/i.p., respectively;n= 14). DMPX was used as an adenosine receptor antagonist to evaluate whether adenosine receptor block might prevent PDRN effects. PDRN systemically administered 1 h following SCI, protected from tissue damage, demyelination, and reduced motor deficits evaluated after 10 days. PDRN also reduced the release of the pro-inflammatory cytokines TNF-α and IL-1β, reduced BAX expression and preserved Bcl-2. Furthermore, PDRN stimulated Wnt/β-catenin pathway and decreased apoptotic process 24 h following SCI, whereas DMPX administration prevented PDRN effects on Wnt/β-catenin signaling. These results confirm PDRN anti-inflammatory activity and demonstrate that a crosstalk between Wnt/β-catenin signaling is possible by adenosine receptors activation. Moreover, these data let us hypothesize that PDRN might promote neural repair through axonal regeneration and/or neurogenesis.
A Haar Wavelet Decision Feedback Channel Estimation Method in OFDM Systems
Authors: Tang Ruiguang , Zhou Xiao , Wang Chengyou
Published: May 2018

Channel estimation is a key technology in improving the performance of the orthogonal frequency division multiplexing (OFDM) system. The pilot-based channel estimation method decreases the spectral efficiency and data transmission rate. Some conventional channel estimation methods cannot suppress the noise effectively, which affects the quality of the final received signals. To solve these two problems, a Haar wavelet decision feedback (DF) channel estimation method, also named the Haar wavelet method, is proposed in this paper. The proposed Haar wavelet method can suppress the noise existing at the estimated channel impulse response (CIR) effectively, based on a time-domain threshold which is a standard deviation of noise obtained by wavelet decomposition. At the same time, the proposed Haar wavelet method just requires inserting one block pilot symbol in front of the first OFDM symbol, which therefore improves the data transmission rate and spectrum efficiency greatly. Simulation results are shown to verify the effectiveness of the proposed Haar wavelet method in multipath channel propagation conditions.
A Tunable Mid-Infrared Solid-State Laser with a Compact Thermal Control System
Authors: Yu Deyang , He Yang , Zhang Kuo , Pan Qikun , Chen Fei , Guo Lihong
Published: May 2018

Tunable mid-infrared lasers are widely used in laser spectroscopy, gas sensing and many other related areas. In order to solve heat dissipation problems and improve the environmental temperature adaptability of solid-state laser sources, a tunable all-fiber laser pumped optical parametric oscillator (OPO) was established, and a compact thermal control system based on thermoelectric coolers, an automatic temperature control circuit, cooling fins, fans and heat pipes was integrated and designed for the laser. This system is compact, light and air-cooling which satisfies the demand for miniaturization of lasers. A mathematical model and method was established to estimate the cooling capacity of this thermal control system under different ambient environments. A finite-element model was built and simulated to analyze the thermal transfer process. Experiments in room and high temperature environments were carried out and showed that the substrate temperature of a pump module could be maintained at a stable value with controlled precision to 0.2 degrees, while the output power stability of the laser was within ±1%. The experimental results indicate that this compact air-cooling thermal control system could effectively solve the heat dissipation problem of mid-infrared solid-state lasers with a one hundred watts level pump module in room and high temperature environments.
The Effects of Low Air Temperatures on Thermoregulation and Sleep of Young Men While Sleeping Using Bedding
Authors: Tsuzuki Kazuyo , Okamoto-Mizuno Kazue , Mizuno Koh
Published: May 2018

Most people do not use heating systems during nocturnal sleep in their bedrooms during the cold season in Japan; instead, they like to use extra blankets and quilts for sleeping. To investigate the effect of low air temperature (Ta) on sleep and thermoregulation when sleeping with bedding, 12 healthy young men participated in sleep experiments over three non-consecutive nights. The experimental conditions were at a Ta of 3 °C, 10 °C and 17 °C, with a relative humidity (Rh) of 50% and an air velocity of <0.2 m/s in the climatic chambers. The 4.3-clo bedding consisted of cotton sheets, an acrylic blanket, a down-filled quilt and a futon mattress. The average sleep efficiency index (SEI) remained >94% for each of the three Tas, and no disturbances were found by Ta in the sleep parameters, although the duration of rapid eye movement (REM) sleep showed variations at 3 °C. The skin temperature (Ts) of the forehead decreased in proportion to Ta and remained stable. The decreasing rate in rectal temperature (Tre), Ts of forehead and thigh during sleep were significantly greater at 3 °C than at 10 °C and 17 °C. The innermost microclimate temperature (Tmicro) made by the bedding did not correlate with the Tre. The young men were significantly more dissatisfied with 3 °C in the sleep quality evaluation.
Development of an Experimental Model for Analyzing Drug Resistance in Colorectal Cancer
Authors: Elbadawy Mohamed , Usui Tatsuya , Yamawaki Hideyuki , Sasaki Kazuaki
Published: May 2018

Colorectal cancer (CRC) is one of the most common cancers, for which combination treatment of chemotherapy is employed. However, most patients develop drug resistance during the course of treatment. To clarify the mechanisms of drug resistance, various research models have been developed. Recently, we established a human CRC patients-derived three-dimensional (3D) culture system using an air-liquid interface organoid method. It contained numerous cancer stem cells and showed resistance to 5-fluorouracil and Irinotecan. In this review, we introduce conventional and our established models for studying drug resistance in CRC.
A Survey on Formal Verification Techniques for Safety-Critical Systems-on-Chip
Authors: Grimm Tomás , Lettnin Djones , Hübner Michael
Published: May 2018

The high degree of miniaturization in the electronics industry has been, for several years, a driver to push embedded systems to different fields and applications. One example is safety-critical systems, where the compactness in the form factor helps to reduce the costs and allows for the implementation of new techniques. The automotive industry is a great example of a safety-critical area with a great rise in the adoption of microelectronics. With it came the creation of the ISO 26262 standard with the goal of guaranteeing a high level of dependability in the designs. Other areas in the safety-critical applications domain have similar standards. However, these standards are mostly guidelines to make sure that designs reach the desired dependability level without explicit instructions. In the end, the success of the design to fulfill the standard is the result of a thorough verification process. Naturally, the goal of any verification team dealing with such important designs is complete coverage as well as standards conformity, but as these are complex hardware, complete functional verification is a difficult task. From the several techniques that exist to verify hardware, where each has its pros and cons, we studied six well-established in academia and in industry. We can divide them into two categories: simulation, which needs extremely large amounts of time, and formal verification, which needs unrealistic amounts of resources. Therefore, we conclude that a hybrid approach offers the best balance between simulation (time) and formal verification (resources).
Assessing the Difference between Soil and Water Assessment Tool (SWAT) Simulated Pre-Development and Observed Developed Loading Regimes
Authors: Zeiger Sean J. , Hubbart Jason A.
Published: May 2018

The purpose of this research was to assess the difference between Soil and Water Assessment Tool (SWAT) simulated pre-development and contemporary developed loading regimes in a mixed-land-use watershed of the central United States (US). Native land cover based on soil characteristics was used to simulate pre-development loading regimes using The Soil and Water Assessment Tool (SWAT). Loading targets were calculated for each major element of a pre-development loading regime. Simulated pre-development conditions were associated with increased retention and decreased export of sediment and nutrients when compared to observed developed conditions. Differences between simulated pre-development and observed developed maximum daily yields (loads per unit area) of suspended sediment (SS), total phosphorus (TP), and total inorganic nitrogen (TIN) ranged from 35.7 to 59.6 Mg km−2 (SS); 23.3 to 52.5 kg km−2 (TP); and, 113.2 to 200.8 kg km−2 (TIN), respectively. Average annual maximum daily load was less during simulated pre-development conditions when compared to observed developed conditions by ranges of 1,307 to 6,452 Mg day−1 (SS), 0.8 to 5.4 kg day−1 (TP), and 4.9 to 26.9 kg day−1 (TIN), respectively. Hydrologic modeling results indicated that the differences in annual maximum daily load were causally linked to land use and land cover influence on sediment and nutrient loading. The differences between SWAT simulated pre-development and observed contemporary loading regimes from this study point to a need for practical loading targets that support contemporary management and integrated flow and pollutant loading regimes.
Relationship between Body Mass Index, Cardiorespiratory and Musculoskeletal Fitness among South African Adolescent Girls
Authors: Bonney Emmanuel , Ferguson Gillian , Smits-Engelsman Bouwien
Published: May 2018

Background: Cardiorespiratory and musculoskeletal fitness are important health indicators that support optimal physical functioning. Understanding the relationship between body mass index and these health markers may contribute to the development of evidence-based interventions to address obesity-related complications. The relationship between body mass index, cardiorespiratory and musculoskeletal fitness has not been well explored, particularly in female adolescents. The aim of this study was to investigate the association between body mass index, cardiorespiratory and musculoskeletal fitness among South African adolescent girls in low-income communities. Methods: This cross-sectional study included 151 adolescent girls, aged 13–16 years. Cardiorespiratory fitness was measured using the 20 m shuttle run test and musculoskeletal fitness was assessed using a variety of field-based tests. Height and weight were measured with standardised procedures and body mass index (BMI) was derived by the formula [BMI = weight (kg)/height (m)2]. Participants were categorised into three BMI groups using the International Obesity Task Force age- and gender-specific cut-off points. Pearson correlations were used to determine the association between body mass index, cardiorespiratory fitness and measures of musculoskeletal fitness at p ≤ 0.05. Results: Overweight and obese girls were found to have lower cardiorespiratory fitness, decreased lower extremity muscular strength, greater grip strength, and more hypermobile joints compared to normal-weight peers. BMI was negatively associated with cardiorespiratory fitness and lower extremity muscular strength. Conclusions: The findings indicate that increased body mass correlates with decreased cardiorespiratory and musculoskeletal fitness. Interventions should be developed to target these important components of physical fitness in this demographic group.
Evaluation of Self-Propelled High-Energy Ultrasonic Atomizer on Azoxystrobin and Tebuconazole Application in Sunlit Greenhouse Tomatoes
Authors: Li Yan-Jie , Li Yi-Fan , Chen Rong-Hua , Li Xue-Sheng , Pan Can-Ping , Song Jian-Li
Published: May 2018

In this study, a self-propelled high-energy ultrasonic atomizer was evaluated in terms of deposition on the canopy, the loss to the ground, and fungicide residues in cherry tomato and tomato. Artificial collectors fixed to the upper side and underside of the leaves at different depths and heights were used to collect the depositions. A reliable analytical method for determination of azoxystrobin and tebuconazole in artificial collectors and residue samples was developed by using liquid chromatography triple-quadrupole mass spectrometry. The results showed that the atomizer distributed the droplets evenly throughout the greenhouse with good uniformity (CVs below 39%). The ratio of depositions on the internal and external sides was 66–83%, and the ratio of depositions on the underside and upper side was 39–50%. There were no significant differences in depositions between two different height crops. The residues of azoxystrobin and tebuconazole in tomato and cherry tomato fruits were far below the maximum residue limits at harvest time. In general, self-propelled high-energy ultrasonic atomizer used in a greenhouse could increase the depositions, especially on the underside and internal side of the canopies, and lead to a reduction of operator exposure risk.
Special Issue “Decision Models in Green Growth and Sustainable Development”
Authors: Zhang Ning , Gong Zaiwu , Yin Kedong , Wang Yuhong
Published: May 2018

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Obstacles and Future Prospects: Considerations on Health Promotion Activities for Older Workers in Europe
Authors: Magnavita Nicola
Published: May 2018

The ageing of workers is one of the most important issues for occupational health and safety in Europe. The ageing of the active population means that health promotion is a necessity rather than a mere option. This review considers barriers and perspectives for workplace health promotion for older workers. Lack of awareness on the part of management and inflexibility in the occupational health and safety system appear to be major barriers. To overcome these, it will be necessary to disseminate knowledge regarding the effectiveness of health promotion actions for older workers, encourage greater involvement on the part of social partners, recover resources by replacing medical consumerism and bureaucratic practices, adopt an integrated approach combining the prevention of occupational risks and the promotion of healthy lifestyles, and recognize subsidiarity and the ability of working communities to regulate themselves.
Stress Response and Cognitive Performance Modulation in Classroom versus Natural Environments: A Quasi-Experimental Pilot Study with Children
Authors: Mygind Lærke , Stevenson Matt P. , Liebst Lasse S. , Konvalinka Ivana , Bentsen Peter
Published: May 2018

Stress during childhood can have mental and somatic health influences that track throughout life. Previous research attributes stress-reducing effects to natural environments, but has mainly focused on adults and often following leisurely relaxation in natural environments. This pilot study explores the impact of natural environments on stress response during rest and mental load and cognitive performance in 47 children aged 10–12 years in a school context. Heart rate variability measures indexing tonic, event, and phasic vagal tone and attention scores were compared across classroom and natural environments. Tonic vagal tone was higher in the natural environment than the classrooms, but no differences were found in event or phasic vagal tone or cognitive performance measures. These findings suggest a situational aspect of the conditions under which natural environments may give rise to stress-buffering influences. Further research is warranted to understand the potential benefits in a real-life context, in particular with respect to the underpinning mechanisms and effects of accumulated exposure over time in settings where children spend large proportions of time in natural environments.
Time to Rethink Refugee and Migrant Health in Europe: Moving from Emergency Response to Integrated and Individualized Health Care Provision for Migrants and Refugees
Authors: Puchner Karl , Karamagioli Evika , Pikouli Anastasia , Tsiamis Costas , Kalogeropoulos Athanasios , Kakalou Eleni , Pavlidou Elena , Pikoulis Emmanouil
Published: May 2018

In the last three years, the European Union (EU) is being confronted with the most significant influx of migrants and refugees since World War II. Although the dimensions of this influx—taking the global scale into account—might be regarded as modest, the institutional response to that phenomenon so far has been suboptimal, including the health sector. While inherent challenges of refugee and migrant (R&M) health are well established, it seems that the EU health response oversees, to a large extend, these aspects. A whole range of emergency-driven health measures have been implemented throughout Europe, yet they are failing to address adequately the changing health needs and specific vulnerabilities of the target population. With the gradual containment of the migratory and refugee waves, three years after the outbreak of the so-called ‘refugee crisis’, we are, more than ever, in need of a sustainable and comprehensive health approach that is aimed at the integration of all of migrants and refugees—that is, both the new and old population groups that are already residing in Europe—in the respective national health systems.
Prognostic Value of RNASEH2A-, CDK1-, and CD151-Related Pathway Gene Profiling for Kidney Cancers
Authors: Yang Chin-An , Huang Hsi-Yuan , Yen Ju-Chen , Chang Jan-Gowth
Published: May 2018

The nucleotide degrading enzyme gene RNASEH2A (ribonuclease H2 subunit A) has been found to be overexpressed in cancers. Our aim was to understand the role of RNASEH2A in cancer prognostication and to establish a scoring system based on the expressions of genes interacting with RNASEH2A. We screened the nucleotide degrading enzyme gene expression in RNAseq data of 14 cancer types derived from The Cancer Genome Atlas (TCGA) and found that RNASEH2A overexpression was associated with poor patient survival only in renal cell carcinomas (RCCs). Further cluster analyses of samples with poor outcomes revealed that cluster of differentiation 151 (CD151) upregulation correlated with low cyclin dependent kinase 1 (CDK1) and high RNASEH2A expression. The combination of low CD151 expression and high RNASEH2A expression resulted in impaired proliferation in four kidney cancer cell lines, suggesting potential synthetic dosage lethality (SDL) interactions between the two genes. A prognostication scoring system was established based on the expression levels of RNASEH2A-, CDK1-, and CD151-related genes, which could effectively predict the overall survival in a TCGA clear cell RCC cohort (n = 533, 995.3 versus 2242.2 days, p < 0.0001), in another clear cell renal cell carcinoma (ccRCC) cohort E-GEOD-22541 (n = 44, 390.0 versus 1889.2 days, p = 0.0007), and in a TCGA papillary RCC (pRCC) cohort (n = 287, 741.6 versus 1623.7 days, p < 0.0001). Our results provide a clinically applicable prognostication scoring system for renal cancers.
Therapeutics for Inflammatory-Related Diseases Based on Plasmon-Activated Water: A Review
Authors: Yang Chih-Ping , Liu Yu-Chuan
Published: May 2018

It is recognized that the properties of liquid water can be markedly different from those of bulk one when it is in contact with hydrophobic surfaces or is confined in nano-environments. Because our knowledge regarding water structure on the molecular level of dynamic equilibrium within a picosecond time scale is far from completeness all of water’s conventionally known properties are based on inert “bulk liquid water” with a tetrahedral hydrogen-bonded structure. Actually, the strength of water’s hydrogen bonds (HBs) decides its properties and activities. In this review, an innovative idea on preparation of metastable plasmon-activated water (PAW) with intrinsically reduced HBs, by letting deionized (DI) water flow through gold-supported nanoparticles (AuNPs) under resonant illumination at room temperature, is reported. Compared to DI water, the created stable PAW can scavenge free hydroxyl and 2,2-diphenyl-1-picrylhydrazyl radicals and effectively reduce NO release from lipopolysaccharide-induced inflammatory cells. Moreover, PAW can dramatically induce a major antioxidative Nrf2 gene in human gingival fibroblasts. This further confirms its cellular antioxidative and anti-inflammatory properties. In addition, innovatively therapeutic strategy of daily drinking PAW on inflammatory-related diseases based on animal disease models is demonstrated, examples being chronic kidney disease (CKD), chronic sleep deprivation (CSD), and lung cancer.
Structural Biology of STAT3 and Its Implications for Anticancer Therapies Development
Authors: Sgrignani Jacopo , Garofalo Maura , Matkovic Milos , Merulla Jessica , Catapano Carlo V. , Cavalli Andrea
Published: May 2018

Transcription factors are proteins able to bind DNA and induce the transcription of specific genes. Consequently, they play a pivotal role in multiple cellular pathways and are frequently over-expressed or dysregulated in cancer. Here, we will focus on a specific “signal transducer and activator of transcription” (STAT3) factor that is involved in several pathologies, including cancer. For long time, the mechanism by which STAT3 exerts its cellular functions has been summarized by a three steps process: (1) Protein phosphorylation by specific kinases, (2) dimerization promoted by phosphorylation, (3) activation of gene expression by the phosphorylated dimer. Consequently, most of the inhibitors reported in literature aimed at blocking phosphorylation and dimerization. However, recent observations reopened the debate and the entire functional mechanism has been revisited stimulating the scientific community to pursue new inhibition strategies. In particular, the dimerization of the unphosphorylated species has been experimentally demonstrated and specific roles proposed also for these dimers. Despite difficulties in the expression and purification of the full length STAT3, structural biology investigations allowed the determination of atomistic structures of STAT3 dimers and several protein domains. Starting from this information, computational methods have been used both to improve the understanding of the STAT3 functional mechanism and to design new inhibitors to be used as anticancer drugs. In this review, we will focus on the contribution of structural biology to understand the roles of STAT3, to design new inhibitors and to suggest new strategies of pharmacological intervention.
Elevated LV Mass and LV Mass Index Sign on the Athlete’s ECG: Athletes’ Hearts are Prone to Ventricular Arrhythmia
Authors: Yılmaz Mücahid , Kayançiçek Hidayet
Published: May 2018

Objectives: Intense exercise elevates all heart chambers’ dimensions, left ventricular mass (LV mass), and left ventricular mass index (LV mass index). The relationship between increased ventricular arrhythmias and sudden cardiac death with LV dilatation and elevated LV mass has been previously demonstrated. We investigated whether sports-related LV dilatation and elevated LV mass and LV mass index cause an increase in ventricular repolarization heterogeneity. Patients and Methods: This prospective observational study recruited 565 participants. There were 226 (female: 28) athletes and 339 (female: 45) healthy controls between 17 and 42 years of age. They were evaluated using 12-lead-electrocardiography and transthoracic echocardiography. Electrocardiograms were obtained at a rate of 50 mm/s and an amplitude of 10 mV, including at least 3 QRS complexes for each derivation. They were taken with 12 standard deviations. Transmural dispersion of repolarization indexes (TDR) (Tp-Te interval, Tp-Te/QT ratio and Tp-Te/QTc ratio, Tp-Te(d)) were measured from precordial derivations. Measurements weretakenwith a program which was generated with MATLAB codes. Results: Tp-Te interval, Tp-Te/QT ratio, Tp-Te/QTc ratio, Tp-Te(d), PW (posterior wall thickness), IVS (interventricular septal thickness), LVEDD (left ventricular end-diastolic diameter), LV mass (left ventricular mass), and LV mass index (left ventricular mass index) for the athlete group were significantly higher than for the control group. Correlation analyses revealed that TDR indexes significantly correlated with PW, IVS, LVEDD, LV mass, and LV mass index. Conclusion: LV mass and LV mass index increase in well-trained athletes, and this increase leads to an increase in TDR indexes. The increased frequency of ventricular arrhythmia and sudden cardiac death may be explained with increasing ventricular repolarization heterogeneity in these individuals.
Consensus in Gestational Diabetes MELLITUS: Looking for the Holy Grail
Authors: Agarwal Mukesh M.
Published: May 2018

The world’s pre-eminent diabetes, obstetric, endocrine, and health organizations advocate a plethora of diverse algorithms for the screening, diagnosis, management, and follow-up of gestational diabetes mellitus (GDM). Additionally, there are regional recommendations of local health societies. Several of these proposals for GDM are contentious because some of them were developed from unscientific studies, based on expert-opinion, catered to preserve resources, and subjectively modified for convenience. Due to the wide variety of choices available, the approach to GDM can be extremely diverse even within the same hospital. This lack of consensus creates major problems in addressing prevalence, complications, efficacy of treatment, and follow-up of GDM. Moreover, it becomes nearly impossible to compare the numerous studies. Furthermore, the lack of consensus confuses the health care providers of obstetric health who look to the experts for guidance. Therefore, a clear, objective, “evidence-based” global approach, which is simple, easy to follow, and validated by corroborative research, is crucial. We contend that, despite decades of research, a single acceptable global guideline is not yet on the horizon.
Residual Fatigue Properties of Asphalt Pavement after Long-Term Field Service
Authors: Cui Peide , Xiao Yue , Fang Mingjing , Chen Zongwu , Yi Mingwei , Li Mingliang
Published: May 2018

Asphalt pavement is widely used for expressways due to its advantages of flexibility, low cost, and easy maintenance. However, pavement failures, including cracking, raveling, and potholes, will appear after long-term service. This research evaluated the residual fatigue properties of asphalt pavement after long-term field service. Fatigue behavior of specimens with different pavement failure types, traffic load, service time, and layers were collected and characterized. Results indicate that after long-term field service, surface layer has a longer fatigue life under small stress levels, but shorter fatigue life under large stress levels. Longer service time results in greater sensitivity to loading stress, while heavier traffic results in shorter fatigue life. Surface and underneath layers present very close fatigue trend lines in some areas, indicating that the fatigue behavior of asphalt mixture in surface and underneath layers are aged to the same extent after eight to ten years of field service.
Waste-Based Pervious Concrete for Climate-Resilient Pavements
Authors: Ho Hsin-Lung , Huang Ran , Hwang Lih-Chuan , Lin Wei-Ting , Hsu Hui-Mi
Published: May 2018

For the sake of environmental protection and circular economy, cement reduction and cement substitutes have become popular research topics, and the application of green materials has become an important issue in the development of building materials. This study developed green pervious concrete using water-quenched blast-furnace slag (BFS) and co-fired fly ash (CFFA) to replace cement. The objectives of this study were to gauge the feasibility of using a non-cement binder in pervious concrete and identify the optimal binder mix design in terms of compressive strength, permeability, and durability. For filled percentage of voids by cement paste (FPVs) of 70%, 80%, and 90%, which mixed with CFFA and BFS as the binder (40 + 60%, 50 + 50%, and 60 + 40%) to create pervious concrete with no cement. The results indicate that the complete (100%) replacement of cement with CFFA and BFS with no alkaline activator could induce hydration, setting, and hardening. After a curing period of 28 days, the compressive strength with different FPVs could reach approximately 90% that of the control cement specimens. The cementless pervious concrete specimens with BFS:CFFA = 7:3 and FPV = 90% presented better engineering properties and permeability.
Coextrusion-Based 3D Plotting of Ceramic Pastes for Porous Calcium Phosphate Scaffolds Comprised of Hollow Filaments
Authors: Jo In-Hwan , Koh Young-Hag , Kim Hyoun-Ee
Published: May 2018

This paper demonstrates the utility of coextrusion-based 3D plotting of ceramic pastes (CoEx-3DP) as a new type of additive manufacturing (AM) technique, which can produce porous calcium phosphate (CaP) ceramic scaffolds comprised of hollow CaP filaments. In this technique, green filaments with a controlled core/shell structure can be produced by coextruding an initial feedrod, comprised of the carbon black (CB) core and CaP shell, through a fine nozzle in an acetone bath and then deposited in a controlled manner according to predetermined paths. In addition, channels in CaP filaments can be created through the removal of the CB cores during heat-treatment. Produced CaP scaffolds had two different types of pores with well-defined geometries: three-dimensionally interconnected pores (~360 × 230 μm2 in sizes) and channels (>100 μm in diameter) in hollow CaP filaments. The porous scaffolds showed high compressive strengths of ~12.3 ± 2.2 MPa at a high porosity of ~73 vol % when compressed parallel to the direction of the hollow CaP filaments. In addition, the mechanical properties of porous CaP scaffolds could be tailored by adjusting their porosity, for example, compressive strengths of 4.8 ± 1.1 MPa at a porosity of ~82 vol %. The porous CaP scaffold showed good biocompatibility, which was assessed by in vitro cell tests, where several the cells adhered to and spread actively with the outer and inner surfaces of the hollow CaP filaments.
Genetic Markers for Coronary Artery Disease
Authors: Veljkovic Nevena , Zaric Bozidarka , Djuric Ilona , Obradovic Milan , Sudar-Milovanovic Emina , Radak Djordje , Isenovic Esma R.
Published: May 2018

Coronary artery disease (CAD) and myocardial infarction (MI) are recognized as leading causes of mortality in developed countries. Although typically associated with behavioral risk factors, such as smoking, sedentary lifestyle, and poor dietary habits, such vascular phenotypes have also long been recognized as being related to genetic background. We review the currently available data concerning genetic markers for CAD in English and non-English articles with English abstracts published between 2003 and 2018. As genetic testing is increasingly available, it may be possible to identify adequate genetic markers representing the risk profile and to use them in a clinical setting.
The Clinical Importance of Cystatin C and Hepatic Artery Resistive Index in Liver Cirrhosis
Authors: Stulic Milos , Culafic Djordje , Obrenovic Radmila , Jankovic Goran , Alempijevic Tamara , Lalosevic Milica , Dostanic Natasa , Kovacevic Sandra , Culafic Milica
Published: May 2018

Background: Data suggest cystatin C (CysC) levels and hepatic artery resistive index (HARI) correspond to the progression of chronic liver disease. We aimed to evaluate the clinical significance of these parameters in assessment of fibrosis in patients with liver cirrhosis. Methods: The cross-sectional study included 63 patients with liver cirrhosis. A control group consisted of 30 age- and gender-matched healthy persons. Results: We confirmed significantly higher values of CysC in patients with cirrhosis compared to control group (p = 0.036). Average value of HARI in the examined group was increased (0.72 ± 0.06) and there was the statistically significant difference compared to controls (0.66 ± 0.03) (p < 0.001). We found statistically significant correlation between HARI and CysC in the study group. Analyzing the possibility of distinguishing healthy subjects from patients with fibrosis, we have found that the area under the curve is far greater in the HARI index than CysC. Comparison of CysC among Child–Pugh stages and correlation with a model for end-stage liver disease (MELD) score showed statistically significant results. Conclusion: We confirmed HARI is a more accurate parameter than CysC in discriminating healthy subjects from patients with fibrosis, while CysC could be a better indicator of the stage of liver cirrhosis.
Liquation Cracking in the Heat-Affected Zone of IN738 Superalloy Weld
Authors: Chen Kai-Cheng , Chen Tai-Cheng , Shiue Ren-Kae , Tsay Leu-Wen
Published: May 2018

The main scope of this study investigated the occurrence of liquation cracking in the heat-affected zone (HAZ) of IN738 superalloy weld, IN738 is widely used in gas turbine blades in land-based power plants. Microstructural examinations showed considerable amounts of γ’ uniformly precipitated in the γ matrix. Electron probe microanalysis (EPMA) maps showed the γ-γ’ colonies were rich in Al and Ti, but lean in other alloy elements. Moreover, the metal carbides (MC), fine borides (M3B2 and M5B3), η-Ni3Ti, σ (Cr-Co) and lamellar Ni7Zr2 intermetallic compounds could be found at the interdendritic boundaries. The fracture morphologies and the corresponding EPMA maps confirmed that the liquation cracking in the HAZ of the IN738 superalloy weld resulted from the presence of complex microconstituents at the interdendritic boundaries.
Predicting the Microstructure of a Valve Head during the Hot Forging of Steel 21-4N
Authors: Ji Hongchao , Huang Xiaomin , Ma Chenjun , Pei Weichi , Liu Jinping , Wang Baoyu
Published: May 2018

Valve microstructure is important during hot forging. Austenitic 21-4N steel is often used in exhaust valves. In this study, the microstructure evolution of the forging valve process was predicted using the internal state variables (i.e., average grain size, recrystallized fraction, and dislocation density) modus for 21-4N. First, 21-4N was subjected to hot compression tests on a Gleeble-1500D and static grain growth tests in a heating furnace. A set of uniform viscoplastic constitutive equations was established based on experimental data. Next, the determined unified constitutive equations were conducted in DEFORM-3D, and the microstructure evolution of 21-4N during forging was calculated. Finally, the simulation results of grain size evolution were validated via experiments. Results showed good consistency between the simulations and experiments. Thus, the models adequately predicted the microstructure evolution.
Antibodies towards Tyrosine Amyloid-Like Fibrils Allow Toxicity Modulation and Cellular Imaging of the Assemblies
Authors: Zaguri Dor , Kreiser Topaz , Shaham-Niv Shira , Gazit Ehud
Published: May 2018

The amino acid tyrosine forms cytotoxic amyloid-like fibrils by molecular self-assembly. However, the production of antibodies towards tyrosine assemblies, reflecting their presentation to the immune system, was not demonstrated yet. Here, we describe the production of antibodies that specifically recognize tyrosine in its fibrillated form. The antibodies were demonstrated to specifically bind self-assembled tyrosine, in contrast to its non-aggregated form or disintegrated fibrils. The antibodies could be used for immunostaining of tyrosine fibrils in cultured cells. Furthermore, confocal microscopy allowed a demonstration of the intracellular presence of the metabolite amyloids in a neuroblastoma cell model. Finally, pre-incubation of tyrosine fibrils with the antibodies resulted in significant reduction in their cytotoxicity. Taken together, we provide an experimental proof for the immunogenicity of tyrosine amyloid fibrillary assemblies. These specific antibodies against tyrosine structures could be further used as a research tool to study the dynamics, toxicity and cellular localization of the assemblies.
Betaine Attenuates Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats via Inhibiting Inflammatory Response
Authors: Yang Jia-mei , Zhou Ru , Zhang Min , Tan Huan-ran , Yu Jian-qiang
Published: May 2018

Background: Pulmonary arterial hypertension (PAH) is characterized by increased pulmonary vascular resistance, leading to right ventricular failure and death. Recent studies have suggested that chronic inflammatory processes are involved in the pathogenesis of PAH. Several studies have demonstrated that betaine possesses outstanding anti-inflammatory effects. However, whether betaine exerts protective effects on PAH by inhibiting inflammatory responses in the lungs needs to be explored. To test our hypothesis, we aimed to investigate the effects of betaine on monocrotaline-induced PAH in rats and attempted to further clarify the possible mechanisms. Methods: PAH was induced by monocrotaline (50 mg/kg) and oral administration of betaine (100, 200, and 400 mg/kg/day). The mean pulmonary arterial pressure, right ventricular systolic pressure, and right ventricle hypertrophy index were used to evaluate the development of PAH. Hematoxylin and eosin staining and Masson staining were performed to measure the extents of vascular remodeling and proliferation in fibrous tissue. Monocyte chemoattractant protein-1 (MCP-1) and endothelin-1 (ET-1) were also detected by immunohistochemical staining. Nuclear factor-κB (NF-κB), tumor necrosis factor alpha (TNF-α), and interleukin-1β (IL-1β) were assessed by Western blot. Results: This study showed that betaine improved the abnormalities in right ventricular systolic pressure, mean pulmonary arterial pressure, right ventricle hypertrophy index, and pulmonary arterial remodeling induced by monocrotaline compared with the PAH group. The levels of MCP-1 and ET-1 also decreased. Western blot indicated that the protein expression levels of NF-κB, TNF-α, and IL-1β significantly decreased (p < 0.01). Conclusion: Our study demonstrated that betaine attenuated PAH through its anti-inflammatory effects. Hence, the present data may offer novel targets and promising pharmacological perspectives for treating monocrotaline-induced PAH.
The Chloroplast Genome of Lilium henrici: Genome Structure and Comparative Analysis
Authors: Liu Hai-Ying , Yu Yan , Deng Yi-Qi , Li Juan , Huang Zi-Xuan , Zhou Song-Dong
Published: May 2018

Lilium henrici Franchet, which belongs to the family Liliaceae, is an endangered plant native to China. The wild populations of L. henrici have been largely reduced by habitat degradation or loss. In our study, we determined the whole chloroplast genome sequence for L. henrici and compared its structure with other Lilium (including Nomocharis) species. The chloroplast genome of L. henrici is a circular structure and 152,784 bp in length. The large single copy and small single copy is 82,429 bp and 17,533 bp in size, respectively, and the inverted repeats are 26,411 bp in size. The L. henrici chloroplast genome contains 116 different genes, including 78 protein coding genes, 30 tRNA genes, 4 rRNA genes, and 4 pseudogenes. There were 51 SSRs detected in the L. henrici chloroplast genome sequence. Genic comparison among L. henrici with other Lilium (including Nomocharis) chloroplast genomes shows that the sequence lengths and gene contents show little variation, the only differences being in three pseudogenes. Phylogenetic analysis revealed that N. pardanthina was a sister species to L. henrici. Overall, this study, providing L. henrici genomic resources and the comparative analysis of Lilium chloroplast genomes, will be beneficial for the evolutionary study and phylogenetic reconstruction of the genus Lilium, molecular barcoding in population genetics.
Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite
Authors: Marc Simona , Kirovski Danijela , Mircu Călin , Hutu Ioan , Otavă Gabriel , Paul Cristina , Boldura Oana Maria , Tulcan Camelia
Published: May 2018

The objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves. Methods: Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that received colostrum without clinoptilolite, and experimental groups I (E1) and II (E2) that received colostrum supplemented with 0.5% and 2% clinoptilolite, respectively. The concentration of total protein and protein fractions (albumin, α1-globulin, α2-globulin, β-globulin and γ-globulin) were analyzed by electrophoresis on cellulose acetate. Results: At hour 30 after birth, concentrations of γ-globulins, β-globulin and total protein in E1 group of calves were higher than in control group by 42.11% (p < 0.05), 28.48% (p > 0.05) and 18.52% (p > 0.05), respectively, and were higher, but not significantly, in group E2 compared to the control group. This was in accordance with a significant lower albumin/globulin ratio in groups E1 and E2 (29.35%, p < 0.05 and 35.87%, p < 0.05, respectively) than in control group at 30 h postpartum, which indicates an obvious increase of the globulins fraction in experimental groups. The conclusion: Clinoptilolite was effective in improving passive transfer in new-born calves, but it was more effective if added in colostrum with a dose of 0.5% than with a dose of 2%.
Four New Lignans from Kadsura Interior and Their Bioactivity
Authors: Liu Jiu-Shi , Zhang Jin , Qi Yao-Dong , Jia Xiao-Guang , Zhang Ben-Gang , Liu Hai-Tao
Published: May 2018

A phytochemical investigation of the stems of Kadsura interior has led to an isolation of four new lignans, named kadsutherin E–H (1–4), together with two known lignans (5–6). The structures of the four new compounds were established on the basis of comprehensive spectroscopic analyses. Compounds 1–6 exhibited inhibition against adenosine diphosphate (ADP) induced platelet aggregation. Among the isolated compounds, kadsutherin F (2) showed the strongest anti-platelet aggregation activity with an inhibition of 49.47%.
Synthesis of 8-Fluoro-3,4-dihydroisoquinoline and Its Transformation to 1,8-Disubstituted Tetrahydroisoquinolines
Authors: Hargitai Csilla , Nagy Tamás , Halász Judit , Simig Gyula , Volk Balázs
Published: May 2018

A simple procedure for the synthesis of 8-fluoro-3,4-dihydroisoquinoline is described below, based on a directed ortho-lithiation reaction. This key intermediate was then applied in various transformations. Fluorine–amine exchange afforded the corresponding 8-amino-3,4-dihydroisoquinolines, suitable starting compounds for the synthesis of 1-substituted 8-amino-tetrahydroisoquinolines. On the other hand, reduction and alkylation reactions of 8-fluoro-3,4-dihydroisoquinoline led to novel 1,2,3,4-tetrahydroisoquinoline derivatives that can be used as building blocks in the synthesis of potential central nervous system drug candidates.
Hyperbranched Polyglycerol Derivatives as Prospective Copper Nanotransporter Candidates
Authors: Quadir Mohiuddin , Fehse Susanne , Multhaup Gerhard , Haag Rainer
Published: May 2018

Hyperbranched polyglycerol (hPG) has been used as a multivalent scaffold to develop a series of nanocarriers capable of high-affinity encapsulation of copper (Cu). A rationally selected set of Cu-complexing motifs has been conjugated to hPG hydroxyl groups to render the constructs potentially usable as exogenous sources of Cu for addressing different pathological conditions associated with Cu-deficiency. We have utilized a newly discovered route to attach Cu-binding domains exclusively within a hPG core by selective differentiation between the primary and secondary hydroxyl groups of the polyol. These hPG-derivatives were found to form a stable complex with Cu ions depending on the type of immobilized ligands and corresponding degree of functionalization. In addition, these Cu-bearing nano-complexes demonstrated moderately cationic surface charge resulting in adjustable protein-binding characteristics and low cellular toxicity profile. We envision that these Cu-loaded hPG nanocarriers can be used as a stable platform to transport the metal ion across the systemic circulation to supply bioavailable quantity of Cu in disease-afflicted tissues.
Antifeedant Effects of Essential Oil, Extracts, and Isolated Sesquiterpenes from Pilgerodendron uviferum (D. Don) Florin Heartwood on Red Clover Borer Hylastinus obscurus (Coleoptera: Curculionidae)
Authors: Espinoza Javier , Urzúa Alejandro , Bardehle Leonardo , Quiroz Andrés , Echeverría Javier , González-Teuber Marcia
Published: May 2018

The beetle Hylastinus obscurus Marsham (Coleoptera: Curculionidae), endemic to Europe and Northern Africa, is one of the most important red clover pests in Chile. As commercial insecticides are less effective against this pest, plant secondary metabolites have been considered as an alternative for its control. Here, we have investigated the chemical composition of essential oil (EO), petroleum ether extract (PEE), and dichloromethane extract (DCME) from Pilgerodendron uviferum heartwood. Additionally, the effects of EO and extracts on the feeding behavior (% of weight shift) of H. obscurus have been evaluated. The composition of EO, PEE, and DCME were analyzed using gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The results showed the presence of a similar mixture of sesquiterpenes in the essential oil and in both of the extracts, which accounted for circa 60% of the total mixture. Sesquiterpenes were further isolated using chromatographic methods and were structurally characterized by optical rotation, GC–MS, FTIR, and 1D and 2D NMR experiments. The physicochemical properties of the isolated sesquiterpenes, including lipophilicity and vapor pressure, were also determined. The sesquiterpenes were identified as the following: (−)-trans-calamenene (1), cadalene (2), (−)-cubenol (3), (−)-epi-cubenol (4), (−)-torreyol (5), and (−)-15-copaenol (6). The antifeedant activity of EO, extracts, and isolated sesquiterpenes were evaluated using artificial diets in a non-choice test. Relative to the control, the EO, DCME extract, and the isolated sesquiterpenes, namely, (−)-trans-calamenene (1), cadalene (2), and (5) torreyol, were found to be the most effective treatments against H. obscurus. Our study showed that the compounds occurring in P. uviferum heartwood were effective in reducing the adult growth of H. obscurus. The physicochemical properties of the isolated sesquiterpenes might have been associated with antifeedant effects.