Prediksi Protein Target Bioaktif Ekstrak Metanol Buah Belimbing (Averrhoa carambola) dalam Regulasi Tekanan Darah
Authors: Munawaroh Siti , Purwaningroom Dian Laila , Putri Dianita Rifqia , Rosjidi Cholik Harun 
Published: October 2020

AbstractBlood pressure regulation is basically control of amount of blood flow to certain tissues according to their metabolic needs. The complexity of the mechanisms involved leading to the assumption that there are many functional and structural proteins involved in blood pressure regulation. Indonesian people, especially those living on the island of Java, usually consume star fruit Averrhoa carambola to reduce blood pressure, but the content of bioactive compounds and the mechanism of their interference with proteins is still unknown. This study aimed to determine the bioactive compound content of Averrhoa carambola's methanol extract and its mechanism of interference with the target proteins. An amount of 200 g Averrhoa carambola dried simplicia was extracted by the maceration method using absolute methanol. The extract was then subjected to phytochemical tests using the Gas Chromatography-Mass Spectrometry (GC-MS) method. Based on the results obtained from the GC-MS phytochemical test, the names of the bioactive compounds found in the Averrhoa carambola methanol extract were obtained. The compounds are then analyzed for their target protein in the human body using the STITCH database (http://stitch.embl.de/). The proteins that had been predicted to be the target of active compounds of Averrhoa carambola methanol extract were analyzed for interactions between proteins using the STRINGdb database (https://string-db.org/cgi/input.pl). Based on this research, it can be concluded that Averrhoa carambola fruit methanol extract can help lower blood pressure by producing NO and acting as antioxidants. However, further research (in-vitro and in-vivo) needs to be done to prove the mechanism.

AbstrakRegulasi tekanan darah adalah jumlah kontrol aliran darah ke jaringan tertentu sesuai dengan kebutuhan metabolismenya. Kompleksnya mekanisme yang terlibat, memunculkan asumsi terdapat banyak protein fungsional maupun struktural yang terlibat dalam regulasi tekanan darah. Masyarakat Indonesia, terutama yang tinggal di pulau Jawa biasa mengkonsumsi buah belimbing untuk menurunkan tekanan darah, namun kandungan senyawa bioaktif dan mekanisme intervensinya terhadap protein-protein belum diketahui. Tujuan penelitian ini adalah untuk mengetahui kandungan senyawa bioaktif ekstrak metanol buah Averrhoa carambola dan mekanisme intervensinya terhadap protein-protein targetnya. Simplisia kering buah Averrhoa carambola sebanyak 200 g diekstraksi dengan metode maserasi menggunakan metanol absolut. Dari ekstrak tersebut dilakukan uji fitokimia dengan metode Gas chromatography–mass spectrometry (GC-MS) sehingga didapatkan nama-nama senyawa bioaktif yang terdapat pada ekstrak metanol Averrhoa carambola. Senyawa-senyawa tersebut kemudian dianalisis protein targetnya dalam tubuh manusia menggunakan database STITCH (http://stitch.embl.de/). Kemudian dianalisis interaksi antar protein menggunakan database STRINGdb (https://string-db.org/cgi/input.pl). Berdasarkan penelitian ini, ekstrak metanol buah Averrhoa carambola dapat membantu menurunkan tekanan darah dengan memproduksi NO dan berperan sebagai antioksidan. Namun, penelitian lebih lanjut (in-vitro dan in-vivo) perlu dilakukan untuk membuktikan mekanismenya