bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Five Soy Proteins on Lean Chicken Breast Systems with Varying Moisture Contents: Cooking Loss, Texture, Microstructure, and T2 NMR
Authors: Lin Weilun , Barbut Shai
Published: January 2025

With increasing global meat consumption, meat–plant hybrid products have gained interest as a sustainable alternative. Soy proteins have been used in small quantities (2–3%) as meat extenders, yet limited data exist on their use at higher levels. Here, five commercial soy proteins (four isolates: SPI-A to -D; one concentrate: SPC) were used for meat replacement in lean meat batters with 0/40/80% added water. Cooking loss, texture, light micrographs, and T2 relaxation were analyzed. At 33% and 66% meat replacement, soy protein treatments maintained comparable or reduced cooking loss; SPI-D and SPC were the least and most effective, respectively. Complete replacement eliminated cooking loss in 0% and 40% water systems but failed to form self-supporting gels in the 80% system. At 33% replacement, SPI-A to -C generally increased hardness, whereas increasing the replacement level further to 66% decreased it. In the 0% and 80% systems, SPI-A treatments exhibited hardness comparable to controls, SPI-D treatments drastically reduced hardness, and SPC treatments maintained greater hardness than the controls even at 66% replacement. Micrographs offered potential explanations for these macroscopic measurements. NMR T2 data indicated that soy proteins restricted water mobility both pre- and post-cooking. Specifically, in the 40% and 80% systems, the T22 peaks (expelled liquid) of the hybrid samples containing 33% SPI-A were ~350 ms and 760 ms, compared to ~570 ms and 1170 ms for the meat controls, respectively. In conclusion, most soy proteins (except SPI-D) enhanced water binding, with SPI-A showing optimal texture and SPC showing promise as a more economical alternative.
Comparison of Volatile and Non-Volatile Compounds of Ice-Stored Large Yellow Croaker (Larimichthys crocea) Affected by Different Post-Harvest Handling Methods
Authors: Zheng Yao , Ma Yuan , Lin Na , Yang Xu , Wu Junjie , Guo Quanyou
Published: January 2025

To compare the impact of different post-harvest handling methods on volatile and non-volatile compounds, a total of 54 live large yellow croakers were subjected to commercial slaughter (CS), spinal cord cutting (SCC), or spinal cord cutting and bleeding (SCCB). The fish samples were ice-stored for 72 h, followed by the analysis of volatile compounds using gas chromatography–ion mobility spectrometry and non-volatile compounds using LC-MS-based untargeted metabolomics. The results revealed the detection of a total of 28 volatile organic compounds, with 23 being successfully identified, predominantly including alcohols, aldehydes, esters, ketones, and heterocyclic compounds. Substances such as (E)-2-nonenal and 2-butanone are highly sensitive to post-harvest handling methods during ice storage. Furthermore, 943 non-volatile metabolites were identified, showing significant differences in 180, 100, 117, and 186 metabolites across comparisons of SCC 0 h/CS 0 h, SCCB 0 h/CS 0 h, SCC 72 h/CS 72 h, and SCCB 72 h/CS 72 h, respectively. Notably, the altered metabolic pathways mainly involved fatty acid and amino acid metabolism, including pathways like glycerophospholipid metabolism and arginine biosynthesis. This study revealed the potential mechanisms underlying the enhancement of fish quality through spinal cord cutting and bleeding.
Asymptotic Periodicity of Bounded Mild Solutions for Evolution Equations with Non-Densely Defined and Fractional Derivative
Authors: Zuo Jiabin , Taqbibt Abdellah , Chaib Mohamed , Elomari M’hamed
Published: January 2025

In the present article, we establish conditions for the asymptotic periodicity of bounded mild solutions in two distinct cases of evolution equations. The first class involves non-densely defined operators, while the second class incorporates densely defined operators with fractional derivatives that generate a semigroup of contractions. Our method integrates the theory of spectral properties of uniformly bounded continuous functions defined on the positive real semi-axis. Additionally, we apply extrapolation theory to evolution equations with non-densely defined operators. To illustrate our main results, we provide a concrete example.
Representation of Special Functions by Multidimensional A- and J-Fractions with Independent Variables
Authors: Dmytryshyn Roman , Sharyn Serhii
Published: January 2025

The paper deals with the problem of representing special functions by branched continued fractions, particularly multidimensional A- and J-fractions with independent variables, which are generalizations of associated continued fractions and Jacobi continued fractions, respectively. A generalized Gragg’s algorithm is constructed that enables us to compute, by the coefficients of the given formal multiple power series, the coefficients of the corresponding multidimensional A- and J-fractions with independent variables. Presented below are numerical experiments for approximating some special functions by these branched continued fractions, which are similar to fractals.
Association of BMP15 and GDF9 Gene Polymorphisms with Litter Size in Hu Sheep
Authors: Zhang Yuting , Wang Haitao , Li Tingting , Zhang Na , Chen Jieran , Yang Hengqian , Peng Shiyu , Ma Runlin , Wang Daxiang , Liu Qiuyue , Wang Yuanyuan
Published: January 2025

(1) Background: Litter size is one of the most important economic traits of sheep. The FecB locus has been extensively studied due to its significant impact on litter size in Hu sheep, and BMP15 and GDF9 have also been reported as major genes associated with litter size in sheep. This study aimed to identify variants of BMP15 and GDF9 and perform an association analysis of these variants with litter size in the Hu sheep breed. (2) Methods: In this study, exons of the BMP15 and GDF9 genes were fully sequenced to identify polymorphisms in Hu sheep. Population genetic parameters and haplotype frequencies were estimated, and an association analysis between these polymorphic loci and litter size was performed. Additionally, the protein structures of the wild-type and mutated BMP15 and GDF9 genes were predicted. (3) Results: The polymorphisms of the BMP15 and GDF9 genes were investigated within their exon regions, revealing mutations at four previously reported sites: BMP15 c.31_33CTTdel and GDF9 (G2, G3, and G4) in Hu sheep, with no novel variants were detected. Genetic analysis indicated that the GDF9-G3 and GDF9-G4 loci have low polymorphisms, whereas the BMP15 c.31_33CTTdel and the GDF9-G2 locus are moderately polymorphic. The mutation sites in the BMP15 and GDF9 genes were under Hardy–Weinberg equilibrium. Association analysis revealed that the BMP15 c.31_33CTTdel and GDF9 (G2, G3, and G4) mutations are not associated with litter size in Hu sheep. Protein structure prediction indicated that the mutations in BMP15 and GDF9 resulted in alterations to their tertiary structures. (4) Conclusions: In this study, four reported mutations in the BMP15 and GDF9 genes can also be detected in the Hu sheep breed. In these mutations, the G2 and G3 mutations of GDF9 did not alter the amino acid sequence, while the BMP15 c.31_33CTTdel mutation and the GDF9 G4 mutation resulted in protein structure alteration. Furthermore, the BMP15 c.31_33CTTdel mutation and the GDF9 mutations (G2, G3, G4) were associated with an increased tendency in litter size. However, no significant difference was observed (p > 0.05). This study provides valuable insights for improving the lambing performance of Hu sheep.
Identifying Caregiving Youth and Associated Mental Health Concerns in a Medical Clinic Setting
Authors: Pulgaron Elizabeth R. , Llano Gabriella , Guevara Gabriela , LaMarca Tara Kenworthy , Wurm Gwen , Gwynn Lisa , Siskowski Connie , Belkowitz Julia
Published: January 2025

Background/ Objectives: Despite the high estimated prevalence and the documented impact of caregiving on children, there is no systematic process to identify or study caregiving youth in the healthcare setting. The aim of this study was to pilot screening in school-based clinics to identify caregiving youth and their associated mental health outcomes. Methods: From March 2021 to March 2022, ninth- to twelfth-grade students were surveyed regarding caregiving and validated mental health screeners during intake at three Title 1 school-based health clinics in Miami, FL. Results: Thirty-nine percent of participants self-identified as caregivers. The most common caregiving tasks were cleaning (n = 20, 50%), keeping company (n = 19, 48%), shopping/cooking (n = 14, 35%), dressing (n = 13, 33%), mobility support (n = 12, 30%), and medical support (n = 11, 28%). Compared to their non-caregiving counterparts, caregiving youth had higher scores on mental health screeners, and caregivers were more likely to endorse clinically significant levels of depression (p = 0.050). Conclusions: Screening in the healthcare system was effective at identifying caregiving youth in school-based clinics whose mental health may be impacted by caregiving responsibilities. Pediatricians should actively screen for both caregiving and mental health concerns. Future studies are needed to ensure the caregiving screening tool is reliable and valid for broad-scale provider use.
Plant Probiotic Potential of Native Rhizobia to Enhance Growth and Sugar Content in Agave tequilana Weber var. Blue
Authors: Maranto-Gómez Víctor Manuel , Rincón-Molina Clara Ivette , Manzano-Gómez Luis Alberto , Gen-Jiménez Adriana , Maldonado-Gómez Julio César , Villalobos-Maldonado Juan José , Ruiz-Valdiviezo Víctor Manuel , Rincón-Rosales Reiner , Rincón-Molina Francisco Alexander
Published: January 2025

Beneficial soil microorganisms, particularly plant probiotic bacteria (PPB), play a pivotal role in promoting plant growth, development, and overall health through root colonization. PPB-based biofertilizers offer a sustainable and eco-friendly alternative to conventional agricultural inputs. This study evaluates the plant probiotic potential of three native bacterial strains Rhizobium sp. ACO-34A, Sinorhizobium mexicanum ITTG R7T, and Sinorhizobium chiapasense ITTG S70T to enhance the growth, quality, and sugar content of Agave tequilana. A comprehensive genomic and functional analysis was conducted for each strain to assess their plant probiotic traits. Additionally, a greenhouse inoculation assay was performed on six-month-old agave seedlings at the “piña” stage to evaluate the effects of these strains on plant growth and sugar content. Comparative genomic analysis revealed that these rhizobial strains harbor genes associated with key plant probiotic traits, reinforcing their role in enhancing plant development. The results demonstrated significant effects (p < 0.05) on growth and sugar content in inoculated plants. ACO-34A increased plant height by 35.4%, fresh weight by 41.5%, and inulin content by 57.3%, while ITTG-R7T showed improvements of 26.4%, 35.2%, and 38.2%, respectively, compared to the control, and ITTG S70T also exhibited enhancements, although to a lesser extent, with increases of 23.5% in plant height, 28.9% in fresh weight, and 31.2% in inulin content. These findings highlight the biofertilizer potential of these native rhizobial strains, particularly Rhizobium sp. ACO-34A, positioning them as promising candidates for the sustainable cultivation of A. tequilana.
Cytomegalovirus Blood DNAemia in Patients with Severe SARS-CoV-2 Pneumonia
Authors: Mesland Jean-Baptiste , Collienne Christine , Montiel Virginie , Werion Alexis , Hantson Philippe , Wittebole Xavier , Laterre Pierre-François , Gerard Ludovic
Published: January 2025

Introduction: Cytomegalovirus (CMV) DNAemia has been described in critically ill patients, including patients with severe acute respiratory syndrome-coronavirus2 (SARS-CoV-2) infection. Our objective is to evaluate the prevalence and clinical impact of CMV DNAemia among patients undergoing invasive mechanical ventilation (IMV) for severe SARS-CoV-2 infection and to explore the association between CMV DNAemia levels and clinical outcomes. Methods: In this retrospective monocentric study, we included patients admitted in a tertiary ICU for severe COVID-19 and who required IMV. We aimed to compare clinical and demographic variables between patients with and without CMV DNAemia. Univariate and Cox regression analyses were performed to identify factors associated with CMV DNAemia. Results: During the study period, CMV blood DNAemia occurred in 30/135 patients (22%). Patients with CMV blood DNAemia had longer ICU and hospital length of stay, as well as longer duration of IMV, and were more likely to have received dexamethasone. However, there was no significant difference in ICU mortality between patients with and without CMV DNAemia (64.8% vs. 56.7%, p = 0.42). The Cox regression analysis showed that dexamethasone was the only factor independently associated with CMV blood DNAemia (HR 4.23 [1.006–17.792], p = 0.049). Conclusions: In patients with severe SARS-CoV-2 pneumonia requiring IMV, CMV DNAemia is common and associated with prolonged ventilation and increased LOS but not with increased mortality.
Cree Food Knowledge and Being Well
Authors: Robin Tabitha , Hart Michael Anthony
Published: January 2025

This paper explores the crucial role of Cree elders’ knowledge in revitalizing Indigenous food sovereignty, focusing on food as a cornerstone of Cree identity, spirituality, and wellbeing. Based on a study of Cree elders in Manitoba in 2020, this paper highlights the depth of Cree food knowledge, intertwined with spiritual practices, language, and land ethics. Using an Indigenous research paradigm, ten Cree elders were interviewed and shared their experiences of traditional communal practices, the detrimental impacts of colonialism on food systems, and the spiritual connections between food, land, and community. The elders emphasized the need for education and the preservation of Cree languages, which encode critical knowledge for sustaining food practices. Through their stories, elders illustrated how food sovereignty is not merely about physical sustenance but involves maintaining sacred relationships and responsibilities to the land and all its inhabitants. This research underscores the importance of Cree knowledge in reclaiming and sustaining Indigenous food systems, essential for the health and resilience of Cree communities.
Worry and Depressive Symptoms in Adolescents with Neurodevelopmental Disorders
Authors: Stewart Suzanne , Houghton Stephen John , Glasgow Ken , Macqueen Leslie
Published: January 2025

Although worry is a normal cognitive process experienced by adolescents, for a significant number, it can reach intense and uncontrollable levels. If left untreated, these worries can lead to significant mental health problems that are maintained into adulthood. Adolescents with neurodevelopmental disorders (NDDs) may be more prone to cognitive biases (that precede worry) and therefore highly vulnerable to worry. Limited research has examined worry in adolescents with NDDs; however, most studies have focused on measuring anxiety. The present research administered an instrument specifically developed to measure worry to 404 10 to 16-year-old mainstream school-aged adolescents, 204 (123 males, 81 females) of whom had a formally diagnosed NDD. A measure of depressive symptoms was also administered. Confirmatory factor analysis revealed a satisfactory fitting model for worry. Multivariate analysis of variance revealed no interaction effects or main effect for worry according to NDD/non-NDD status. There were, however, main effects for sex, with females scoring significantly higher than males on worry about academic success and the future; worry about peer relationships; combined worry score; and depressive symptoms. The findings of this study offer psychologists and educators a brief validated measure of worry that is suited to mainstream school adolescents with or without NDDs. The wider implications of the findings in the context of education and intervention for students with NDDs are discussed.
Use of Semantic Web Technologies to Enhance the Integration and Interoperability of Environmental Geospatial Data: A Framework Based on Ontology-Based Data Access
Authors: Ranatunga Sajith , Ødegård Rune Strand , Jetlund Knut , Onstein Erling
Published: January 2025

This study addresses the challenges of integrating heterogeneous environmental geospatial data by proposing a framework based on ontology-based data access (OBDA). Geospatial data are important for decision-making in various domains, such as environmental monitoring, disaster management, and urban development. Data integration is a common challenge within these domains due to data heterogeneity and semantic discrepancies. The proposed framework uses semantic web technologies to enhance data interoperability, accessibility, and usability. Several practical examples were demonstrated to validate its effectiveness. These examples were based in Lake Mjøsa, Norway, addressing both spatial and non-spatial scenarios to test the framework’s potential. By extending the GeoSPARQL ontology, the framework supports SPARQL queries to retrieve information based on user requirements. A web-based SPARQL Query Interface (SQI) was developed to execute queries and display the retrieved data in tabular and visual format. Utilizing free and open-source software (FOSS), the framework is easily replicable for stakeholders and researchers. Despite some limitations, the study concludes that the framework is able to enhance cross-domain data integration and semantic querying in various informed decision-making scenarios.
Adult Leigh Syndrome Associated with the m.15635T>C Mitochondrial DNA Variant Affecting the Cytochrome b (MT-CYB) Gene
Authors: Tropeano Concetta Valentina , La Morgia Chiara , Achilli Alessandro , Iommarini Luisa , Tioli Gaia , Caporali Leonardo , Olivieri Anna , Valentino Maria Lucia , Liguori Rocco , Barboni Piero , Martinuzzi Andrea , Tonon Caterina , Lodi Raffaele , Torroni Antonio , Carelli Valerio , Ghelli Anna Maria
Published: January 2025

We report on a sporadic patient suffering Leigh syndrome characterized by bilateral lesions in the lenticular nuclei and spastic dystonia, intellectual disability, sensorineural deafness, hypertrophic cardiomyopathy, exercise intolerance, and retinitis pigmentosa. Complete sequencing of mitochondrial DNA revealed the heteroplasmic nucleotide change m.15635T>C affecting a highly conserved amino acid position (p.Ser297Pro) in the cytochrome b (MT-CYB) gene on a haplogroup K1c1a background, which includes a set of four non-synonymous polymorphisms also present in the same gene. Biochemical studies documented respiratory chain impairment due to complex III defect. This variant fulfils the criteria for being pathogenic and was previously reported in a sporadic case of fatal neonatal polyvisceral failure.
Reinforcing Nrf2 Signaling: Help in the Alzheimer’s Disease Context
Authors: la Torre Annamaria , Lo Vecchio Filomena , Angelillis Valentina Soccorsa , Gravina Carolina , D’Onofrio Grazia , Greco Antonio
Published: January 2025

Oxidative stress plays a role in various pathophysiological diseases, including neurogenerative diseases, such as Alzheimer′s disease (AD), which is the most prevalent neuro-pathology in the aging population. Oxidative stress has been reported to be one of the earliest pathological alterations in AD. Additionally, it was demonstrated that in older adults, there is a loss of free radical scavenging ability. The Nrf2 transcription factor is a key regulator in antioxidant defense systems, but, with aging, both the amount and the transcriptional activity of Nrf2 decrease. With the available treatments for AD being poorly effective, reinforcing the antioxidant defense systems via the Nrf2 pathway may be a way to prevent and treat AD. To highlight the predominant role of Nrf2 signaling in defending against oxidative stress and, therefore, against neurotoxicity, we present an overview of the natural compounds that exert their own neuroprotective roles through the activation of the Nrf2 pathway. This review is an opportunity to promote a holistic approach in the treatment of AD and to highlight the need to further refine the development of new potential Nrf2-targeting drugs.
Differences in GIP Receptor Expression by Feeding Status in the Mouse Brain
Authors: Song Do Kyeong , Jung Narae , Sung Yeon-Ah , Hong Young Sun , Lee Hyejin
Published: January 2025

Gastric inhibitory polypeptide (GIP) contributes to energy metabolism regulation. We investigated differences in GIP receptor expression in the brain by feeding status among lean and obese mice and the effect of acute central GIP administration on the expression of appetite-regulating hypothalamic neuropeptides. We divided the mice into four groups: fed/lean, fasted/lean, fed/obese, and fasted/obese. The arcuate nucleus (ARC), paraventricular nucleus of the hypothalamus, and nucleus of the solitary tract in the brainstem were harvested. GIP (6 nmol) or saline was injected for the acute intracerebroventricular administration test, followed by the collection of hypothalamic tissue after 2 h. Fed/obese mice had higher ARC GIP receptor mRNA levels than fasted/obese and lean mice. This difference was not observed among lean mice by feeding status. Obese mice had higher blood GIP levels than lean mice. Fed/obese mice had higher blood GIP levels than fasted/obese mice. This difference was not observed among lean mice by feeding status. GIP administration significantly increased proopiomelano-cortin (Pomc) mRNA levels (GIP: 7.59 ± 0.14; saline: 3.44 ± 1.38 arbitrary units; p = 0.030). Increased GIP receptor expression in the ARC in obese mice indicates its central nervous system involvement in energy balance regulation. GIP potentially regulates POMC-mediated appetite regulation in the hypothalamus. It is possible that POMC neurons are targets of GIP action in the brain.
Combined Bulked Segregant Analysis-Sequencing and Transcriptome Analysis to Identify Candidate Genes Associated with Cold Stress in Brassica napus L
Authors: Jiang Jiayi , Li Rihui , Wang Kaixuan , Xu Yifeng , Lu Hejun , Zhang Dongqing
Published: January 2025

Cold tolerance in rapeseed is closely related to its growth, yield, and geographical distribution. However, the mechanisms underlying cold resistance in rapeseed remain unclear. This study aimed to explore cold resistance genes and provide new insights into the molecular mechanisms of cold resistance in rapeseed. Rapeseed M98 (cold-sensitive line) and D1 (cold-tolerant line) were used as parental lines. In their F2 population, 30 seedlings with the lowest cold damage levels and 30 with the highest cold damage levels were selected to construct cold-tolerant and cold-sensitive pools, respectively. The two pools and parental lines were analyzed using bulk segregant sequencing (BSA-seq). The G’-value analysis indicated a single peak on Chromosome C09 as the candidate interval, which had a 2.59 Mb segment with 69 candidate genes. Combined time-course and weighted gene co-expression network analyses were performed at seven time points to reveal the genetic basis of the two-parent response to low temperatures. Twelve differentially expressed genes primarily involved in plant cold resistance were identified. Combined BSA-seq and transcriptome analysis revealed BnaC09G0354200ZS, BnaC09G0353200ZS, and BnaC09G0356600ZS as the candidate genes. Quantitative real-time PCR validation of the candidate genes was consistent with RNA-seq. This study facilitates the exploration of cold tolerance mechanisms in rapeseed.
Chiral Layered Zinc Phosphonates: Exfoliation and Chiroptical Properties
Authors: Yang Zi-Xin , Wu Sheng-Fu , Bao Song-Song , Ma Xiu-Fang , Zheng Tao , Zheng Li-Min
Published: January 2025

Chiral layered coordination polymers have attracted considerable attention not only because of their intriguing physicochemical properties but also because of their ability to exfoliate into chiral nanosheets. Chiral metal phosphonates with layered structures are of particular interest due to their relatively high thermal and water stabilities, but their corresponding nanosheets are rarely reported on. Herein, we report on a pair of enantiopure zinc phosphonates with the formula S- and R-Zn8(cyampH)8Cl8 (S-Zn, R-Zn), where S- and R-cyampH2 represent S- and R-(1-cyclohexylamino)methylphosphonic acids. They have layered structures in which the {ZnO3Cl} tetrahedra are connected by {PO3C} tetrahedra via corner-sharing. By doping analogous chromophore ligands S- and R-(1-naphthalenethylamino)methylphosphonic acid (S-, R-nempH2), we obtained compounds S- and R-Zn8(cyampH)8-n(nempH)nCl8 (S-, R-Zn-x%, x = 2, 4, 6), which exhibited obvious circular dichroism (CD) and circularly polarized luminescence (CPL) properties. The bulk samples of S-Zn and S-Zn-4% were further subjected to exfoliation in acetone, resulting in chiral nanosheets of one–three-layer thicknesses.
Association of Immune-Mediated Inflammatory Diseases and Fracture Risk in Patients with Type 2 Diabetes: A Nationwide Population-Based Study
Authors: Kim Yuna , Kwon Oh Chan , Kim Ryul , Kim Jong Hoon , Kim Min-Jae , Park Min-Chan , Kim Jie-Hyun , Youn Young Hoon , Park Hyojin , Han Kyungdo , Chun Jaeyoung
Published: January 2025

Background: Immune-mediated inflammatory diseases (IMIDs) are associated with chronic inflammation that may increase fracture risk; however, their impact within specific populations, such as those with type 2 diabetes mellitus (T2DM), remains unclear. This study aimed to evaluate the association between IMIDs and fracture risk, leveraging a large, high-quality population-based cohort of patients with T2DM. Methods: A total of 2,120,900 patients with T2DM without a previous history of fractures were enrolled from the nationwide health check-up database provided by the Korean National Health Insurance Service. The outcomes were overall, osteoporotic, non-osteoporotic, vertebral and hip fractures. Multivariable Cox proportional hazard regression analysis was performed to estimate the adjusted hazard ratios (aHRs) with 95% confidence intervals (CIs) according to the presence of IMIDs. Results: The presence of ulcerative colitis (aHR: 1.31), rheumatoid arthritis (aHR: 1.19), ankylosing spondylitis (aHR: 1.32), and psoriasis (aHR: 1.14) were significantly associated with the risk of overall fractures. Compared with controls, patients with a single IMID (aHR: 1.18) and at least two IMIDs (aHR: 1.29) had a significantly increased risk of overall fractures, showing a dose–response relationship. Similar results were observed for osteoporotic, vertebral, and hip fractures. Conclusions: The presence of IMIDs in patients with T2DM was associated with an increased risk of fractures, particularly osteoporotic, vertebral, and hip fractures. This study highlights the significant impact of IMIDs on fracture risk within a diabetic population, emphasizing the need for careful monitoring and tailored management strategies.
Colistin Use for the Treatment of Multi-Drug-Resistant Gram-Negative Severe Infections in ICU Patients: A Single-Center Study
Authors: Rojek Stanislaw Wojciech , Wojtowicz Iga , Taccone Fabio Silvio , Duszynska Wieslawa
Published: January 2025

Background: Colistin is increasingly used to treat severe infections caused by multi-drug-resistant (MDR) bacteria, particularly in critically ill patients. Its effectiveness, especially in monotherapy, remains controversial. This study aimed to evaluate the effectiveness and toxicity of colistin therapy in severe MDR infections. Methods: This retrospective study included patients treated with colistin (CMS) at the ICU. Patients’ treatments were divided into four subgroups: monotherapy vs. combination therapy, empirical vs. targeted therapy, intravenous vs. intravenous plus inhaled therapy, and standard doses with and without a loading dose. The primary outcome was clinical cure. Secondary outcomes included microbiological eradication, survival rate, and drug-related toxicity, particularly acute kidney injury (AKI). Exclusion criteria included Gram-positive infection, inhaled therapy alone, use of colistin <5 days. Results: A total of 150 patients (mean age 60 ± 18 years, APACHE II score 17 ± 10) were included. The most frequent condition was hospital-acquired pneumonia (n = 140, 93.3%). The most common pathogen was MDR Acinetobacter baumannii (n = 146, 97.3%). In most patients, colistin therapy was targeted (n = 113, 75.3%) and combined with other antibiotics (n = 124, 82.7%). Inhaled CMS was added in 47 (31.3%) patients. Mean duration of therapy was 10 ± 4 days. Clinical cure occurred in 64 (42.7%) patients, microbiological eradication in 20 (13.3%). AKI developed in 65 (53.7%) patients. Inhaled CMS improved the clinical cure rates (57.4% vs. 37.0%, p = 0.003). Conclusions: Intravenous CMS was mainly used for MDR Acinetobacter baumannii-related pneumonia. Clinical cure was observed in 42.7% of patients, but renal toxicity was high. Combining intravenous and inhaled CMS may improve outcomes.
Recent Advances and Clinical Outcomes of Endometriosis
Authors: Krentel Harald , De Wilde Rudy Leon , de Wilde Maya Sophie
Published: January 2025

Do recent advances positively influence clinical outcomes [...]
Critical Ischemia Following Hyaluronic Acid Filler Injection: A Case Report
Authors: Turcza Jakub Filip , Bartosinska Joanna , Raczkiewicz Dorota
Published: January 2025

Background: Vascular complications, following the application of non-permanent, hyaluronic acid-based tissue fillers are a rare but very serious and rapidly progressive disorder that can, in extreme cases, lead to skin necrosis, blindness, or a stroke. Interest in aesthetic procedures is constantly growing, so awareness and knowledge of the correct and comprehensive treatment of complications are important. The human face is an area characterised by complex innervation and vascularisation. There are high-risk areas in which the application of fillers should be carried out with particular care using appropriate techniques and instruments, as well as preparations with specific rheology. The aetiopathogenesis of vascular complications is complex—involving partial or complete vessel occlusion, the presence of local inflammation in the affected tissues, and potential arteriospasm of the vessels supplying the area, resulting in tissue dysperfusion and ischaemia. Methods: In this article, the case of a patient who developed signs of a vascular compromise on the forehead area after improperly administering filler in the glabellar and nasal areas is presented. Result: The diagnostic and therapeutic management applied, including above all high doses of hyaluronidase, Doppler ultrasound diagnostics, and general medications, as well as a complementary treatment of the distant effects of the complication, i.e., erythema and tissue loss in the forehead area, by autologous injection procedures and laser therapy, resulted in a full recovery and a very good aesthetic result. Conclusions: This case proves that complications after aesthetic medicine procedures, including vascular complications are possible. Education of patients and doctors, proper diagnosis, and initiation of appropriate treatment at an early stage of the problem can bring very good therapeutic results for the patient.
Patient Recruitment Characteristics for Wearable-Sensor-Based Outcome Assessment in Trauma Surgery
Authors: Braun Benedikt J. , Hofmann Kira , Meierhofer Chiara N. , Menger Maximilian M. , Maisenbacher Tanja C. , Vogel Carolina , Haas Dannik , Marmor Meir T. , Histing Tina , Braun Eva-Marie ,   The AO Smart Digital Solutions Task Force
Published: January 2025

Background/Objectives: Using a patient’s own wearable sensor to obtain objective outcome data is a growing field in trauma allowing for the assessment of the recovery trajectory back toward the pre-injury performance. The aim of this study was to analyze recruitment characteristics and reasons for declined study participation in an orthopedic trauma study that measures postoperative recovery using wearables. Methods: Data from 225 patients screened for participation in a wearable-sensor-based outcome study were assessed. The influence of age, sex and injury location on study participation was analyzed. Reasons for patients declining to participate were investigated from the screening log. Availability and type of sensor system in patients agreeing to participate were analyzed. Results: Overall, 48% of patients agreed to participate. Age was the only significantly different factor between agreeing and declining patients (p < 0.05). The main reasons to decline study participation were technical difficulties with or inability to use the wearable device, lack of availability of a wearable, and general disinterest to participate in a study. Notably, 7% declined due to data safety concerns. Conclusions: The results show that age, availability of the wearable, and technical ability to use a wearable are the main obstacles impacting objective outcome measurement using a personal wearable device. In studies including geriatric patients, a dedicated device requiring no patient handling can be a valid option to improve enrollment. Understanding the reasons for declining to participate will facilitate the development of future sensor-based studies to address concerns of technical handling through alternative means of data harvesting and increase the inclusion rate. These outcomes will guide future study designs to optimize patient inclusion.
Albumin–Bilirubin (ALBI) Score Predicts Long-Term Survival in Elderly Patients with Decompensated Heart Failure
Authors: Jurkiewicz Michał , Szczurek-Wasilewicz Wioletta , Skrzypek Michał , Krych Sebastian , Gąsior Mariusz , Szyguła-Jurkiewicz Bożena
Published: January 2025

Background/Objective: Risk stratification in elderly patients with heart failure (HF) is very difficult. There is a lack of reliable tools for assessing the risk of death in this population of patients. The aim of this study was to determine the association between albumin–bilirubin (ALBI) score and long-term mortality in hospitalized elderly patients with decompensated HF. Methods: The study included 242 consecutive HF patients aged over 65 years hospitalized for worsening chronic HF at our institution between 2019 and 2023. The ALBI score was calculated according to the appropriate formula. Results: The median (IQR) age of the study population was 68.00 (range 66.0 to 74.6) years and 21.1% were female. The mean follow-up time was 352 ± 293 days. During the follow-up period, 47.1% patients died. The ALBI score generated good prognostic power (area under the curve = 0.822, p < 0.01) and specificity (86%) as well as acceptable sensitivity (68%) when predicting long-term mortality. Patients with higher ALBI scores (≥−2.191) had significantly worse long-term survival than patients with lower ALBI scores (<−2.191) [37 (25.2%) vs. 77 (81.1%); log rank p < 0.001]. Conclusions: This is the first study demonstrating that ALBI score has good prognostic power and allows for the successful prediction of mortality during long-term follow-up in the analyzed cohort.
Clinical Data Mega-Collection of Obesity and Obesity-Related Trials: Primary Inclusion Criteria from All Studies and Highlights of Clinical Efficacy Analysis of GLP-1 Drugs
Authors: Nguyen Trung Tin , Elmaleh David R.
Published: January 2025

Background/Objectives: Obesity is heterogeneous and considered a chronic epidemic with significant un-met needs for management, treatment, and prevention. Methods: In this study, we used LizAI’s software TAITAN (alpha version) for the mega-collection and analysis of clinical data from 10,407 trials addressing obesity and obesity-related diseases and their associated publications, mainly on PubMed. Results: We report an intensive growth of clinical trials until the end of 2024 and highlight the use of the body mass index (BMI) as a critical criterion in clinical participant selection despite its limitations. The significant disparities in races, regions, and the sites of trials across all studies have not been addressed, posing the possibility of research in the far future on the applications of precision medicine in weight management. In the latter parts of this paper, we analyze and discuss the clinical efficacy, mainly focusing on the primary endpoints and benchmarks of the recently FDA-approved once-weekly injectable glucagon-like peptide-1 receptor agonist (GLP-1 RA) drugs, including semaglutide and tirzepatide. Both drugs have functioned comparably when considering the 5% weight loss FDA threshold. Tirzepatide outperforms semaglutide and impacts fewer participants as the weight loss level increases from 5 to 20% and has greater effects in different populations, especially in people with type 2 diabetes (T2D). Conclusions: We would, however, like to highlight that (i) the weight loss level should be dependent on the clinically relevant needs of patients, and faster and greater weight loss might not be a win, and (ii) the clinical benefits, safety, and quality of life of patients should be carefully assessed when the weight loss is significant in a short period. In our search, we found that the specificities and impacts of weight loss therapies on organs like the kidneys and heart, different muscle types, bones, and fat accumulation in different parts of body were not investigated or disclosed during the clinical study period and longer term monitoring. In light of scientific needs and remarkable public interest in weight loss, our report provides findings on the buzz around losing weight in clinical trials, and our TAITAN software continues to collect data in real time and enrich its knowledge for future updates.
Prediction of Ablation Index and Lesion Size Index for Local Impedance Drop-Guided Ablation
Authors: Sprenger Lukas , Moser Fabian , Maslova Vera , Zaman Adrian , Nonnenmacher Marc , Willert Sven , Frank Derk , Lian Evgeny
Published: January 2025

(1) Background: The effectiveness of RF ablation for PVI depends on the lesion location and size to achieve continuous and durable lesion lines. AI and LSI are widely accepted lesion metrics for guiding the ablation procedure. LI dynamics is another parameter that guides PVI and does not rely on input variables. Limited data are available on a direct comparison between lesion metrics. Our study aims to compare RF application durations and influencing factors during index-guided (AI and LSI) and LI-guided approaches by predicting lesion metrics using machine learning. (2) Methods: While the coefficients in AI and LSI formulas are not disclosed, we trained custom machine-learning models based on Random Forest and Gradient Boosting Regressors to predict AI and LSI metrics for LI-guided ablations. (3) Results: The median RF application durations differed significantly between the lesion metrics, with 7.32, 19.91, and 11.92 s for AI-, LSI-, and LI-guided procedures, respectively. Mean CF was found to be an important predictor of RF application duration for the AI- and LSI-guided approaches. (4) Conclusions: Depending on the metric used, the significant differences in RF application durations suggest that an AI-guide approach may allow for shorter RF application durations, followed by LSI-guided and LI-guided procedures. Further studies are needed to evaluate the safety and efficacy of these results in a clinical setting.
Overview of Systematic Reviews on Septic Arthritis of the Temporomandibular Joint (SATMJ)
Authors: Lubecka Karolina , Galant Kacper , Chęciński Maciej , Chęcińska Kamila , Bliźniak Filip , Ciosek Agata , Gładysz Tomasz , Cholewa-Kowalska Katarzyna , Chlubek Dariusz , Sikora Maciej
Published: January 2025

Objectives: This overview of systematic reviews was carried out following the PRIOR guidelines. It aimed to collect and compare the results of systematic reviews on the etiology, diagnosis, and treatment standards of septic arthritis of the temporomandibular joint. Methods: ACM, BASE, Google Scholar, PubMed, and Scopus were searched on 5 January 2025, for systematic reviews on SATMJ etiology and treatment. Records underwent selection, AMSTAR 2 evaluation, data extraction, and qualitative synthesis. Results: Three systematic reviews were included, covering 38 reports (93 cases), 37 reports (91 cases), and 25 reports (40 cases), respectively. There are seven source reports common to all three reviews. The reviews co-indicate possible odontogenic etiology, differ in opinions about the impact of chronic diseases, and agree on the superiority of pharmacotherapy, though without consensus on specific antibiotics. Severe complications of SATMJ, including potentially lethal ones, were reported. Conclusions: SATMJ is a serious condition requiring urgent and precise medical intervention, yet no clear management guidelines exist. The low overlap and inconsistency of the previous systematic reviews provide a foundation for a more comprehensive synthesis.
Integration of FTIR Spectroscopy and Machine Learning for Kidney Allograft Rejection: A Complementary Diagnostic Tool
Authors: Ramalhete Luís , Araújo Rúben , Vieira Miguel Bigotte , Vigia Emanuel , Aires Inês , Ferreira Aníbal , Calado Cecília R. C.
Published: January 2025

Background: Kidney transplantation is a life-saving treatment for end-stage kidney disease, but allograft rejection remains a critical challenge, requiring accurate and timely diagnosis. The study aims to evaluate the integration of Fourier Transform Infrared (FTIR) spectroscopy and machine learning algorithms as a minimally invasive method to detect kidney allograft rejection and differentiate between T Cell-Mediated Rejection (TCMR) and Antibody-Mediated Rejection (AMR). Additionally, the goal is to discriminate these rejection types aiming to develop a reliable decision-making support tool. Methods: This retrospective study included 41 kidney transplant recipients and analyzed 81 serum samples matched to corresponding allograft biopsies. FTIR spectroscopy was applied to pre-biopsy serum samples, and Naïve Bayes classification models were developed to distinguish rejection from non-rejection and classify rejection types. Data preprocessing involved, e.g., atmospheric compensation, second derivative, and feature selection using Fast Correlation-Based Filter for spectral regions 600–1900 cm−1 and 2800–3400 cm−1. Model performance was assessed via area under the receiver operating characteristic curve (AUC-ROC), sensitivity, specificity, and accuracy. Results: The Naïve Bayes model achieved an AUC-ROC of 0.945 in classifying rejection versus non-rejection and AUC-ROC of 0.989 in distinguishing TCMR from AMR. Feature selection significantly improved model performance, identifying key spectral wavenumbers associated with rejection mechanisms. This approach demonstrated high sensitivity and specificity for both classification tasks. Conclusions: The integration of FTIR spectroscopy with machine learning may provide a promising, minimally invasive method for early detection and precise classification of kidney allograft rejection. Further validation in larger, more diverse populations is needed to confirm these findings’ reliability.
A Linear Periodized Resistance Training Program Is Effective at Reducing Depressive Symptoms but Not Anxiety in Females: A Pilot
Authors: Sawyer Jason , McPherson Matthew , Reuter Meghan , Cacolice Paul A.
Published: January 2025

Background/Objectives: The purpose of this study was to determine whether a periodized resistance training program would influence self-reported depression and anxiety scores in college-aged females. Methods: Eight participants participated in a six-week periodized resistance training program. The participants completed a 3–5 repetition maximum (3–5 RM) for the sumo deadlift (SDL), bench press (BP), barbell back squat (BBS), and standing shoulder press (SSP). These data were used to estimate the 1 repetition maximum (1RM), which in turn was used to develop the periodization program. Following baseline testing, participants participated in two full-body workouts per week for six weeks. Each individual was retested after they completed the 6-week program, performing 3–5 RM for the SDL, BP, BBS, and SSP. To determine symptoms of depression and anxiety, the Beck Depression Inventory (BDI) and Beck Anxiety Inventory (BAI) were distributed before and after participating in the resistance training program. A repeated measures 2 × 2 Analysis of Variance (ANOVA) was used to determine the effect of resistance training had on the outcome measures. Results: There was a significant (p = 0.011) decrease in BDI scores after the 6 weeks of resistance training. There was no statistically significant difference in the BAI scores (p = 0.106). There was no correlation between any individual exercise and the outcome scores. Conclusions: The results of the current study indicate that a periodized resistance training program is effective at reducing self-reported measures of depression using the BDI in college-aged females.
Drug-Induced Serious Cutaneous Reactions in Hospitalized Patients: A Cross-Sectional Study
Authors: Jiamsathit Warisara , Bunarong Kansuda , Papenkort Sonthiya , Cox Anthony R. , Jarernsiripornkul Narumol
Published: January 2025

Background: Serious adverse drug reactions (ADRs) can lead to hospital admission and can be fatal, but some of them are preventable. This study aimed to determine the types and frequencies of serious cutaneous ADRs and the methods employed to manage and prevent them, as well as to assess the factors related to their seriousness. Methods: A cross-sectional study was conducted retrospectively on inpatients and outpatients at a tertiary care hospital. All data were collected from the medical records database over a period of 3 years. Serious cutaneous ADRs were identified in the hospital database using the International Classification of Disease and Related Health Problems, 10th Revision (ICD-10). Results: A total of 2151 cases were retrieved using the ICD-10, and 436 patients were randomly selected for this study. Of these, 218 patients experienced ADRs (50.0%). The major clinical symptoms of the eight serious ADRs included anaphylaxis (38.5%) and urticaria (30.2%). The most commonly suspected drug group was antibiotics (45.0%). The main methods of ADR management were drug treatment (84.4%) and drug withdrawal (81.2%). The primary method of ADR prevention was patient drug allergy cards (52.3%). Factors affecting the severity of ADRs were having an underlying condition (p = 0.031) and the concomitant use of drugs (p = 0.044). Conclusions: Anaphylaxis was the most common serious ADR. Patients with underlying diseases and those taking concomitant drugs are more likely to present with serious ADRs. The prevention of serious ADRs should be promoted at all levels in hospitals to reduce harm and prevent their reoccurrence.