bluuebunny/crossref_metadata_2025 huggingface dataset:
Authentification de l’alimentation des ruminants à partir de la composition de leurs produits et tissus
Authors: PRACHE S. , MARTIN B. , NOZIERE P. , ENGEL E. , BESLE J.M. , FERLAY A. , MICOL D. , CORNU A. , CASSAR-MALEK I. , ANDUEZA D.
Published: May 2020

Les consommateurs sont demandeurs d’informations et de garanties sur les conditions d’élevage des animaux et en particulier sur leur alimentation. Ces préoccupations conduisent les filières animales à prendre des engagements sur les conditions d’élevage et d’alimentation des animaux, engagements spécifiés dans des cahiers des charges. Il apparaît ainsi un besoin d’outils analytiques permettant de garantir le respect de ces engagements et/ou d’aider à les instruire. La nature de l’alimentation influence fortement la composition des tissus et produits animaux. Ceci est dû à des composés spécifiques qui sont soit directement transférés de l’aliment au produit, soit transformés ou produits par les microorganismes du rumen ou le métabolisme animal sous l’effet de l’alimentation. Certains de ces composés peuvent donc, en retour, être utilisés pour tracer l’alimentation. De plus, les différences de composition des tissus et produits provoquent des modifications de leurs propriétés optiques, qui peuvent également être mises à profit. Cet article présente les différentes approches analytiques développées actuellement, les principaux résultats obtenus lors de la comparaison d’alimentations contrastées, puis lors de l’alternance de régimes alimentaires avec les questions de latence et de persistance des biomarqueurs d’intérêt, et enfin quelques exemples d’application sur le terrain avec certaines filières animales intéressées par ces outils.
Mode de conduite en élevage et tendreté de la viande bovine
Authors: OURY M.P. , PICARD B. , ISTASSE L. , MICOL D. , DUMONT R.
Published: May 2020

La tendreté est un paramètre important dans l’évaluation de la qualité de la viande bovine. Elle est sous la dépendance d’une combinaison complexe de paramètres, parmi lesquelles les facteurs technologiques jouent un rôle essentiel. Cependant, lorsque l’environnement péri-abattage est maîtrisé, il demeure une variabilité de la tendreté des viandes, mal perçue par le consommateur, et qui peut être attribuée aux facteurs d’élevage. Au sein d’une même race, le type morphologique n’a pas d’impact sur la tendreté des viandes et la force de cisaillement des muscles. La tendreté et la force de cisaillement de la viande de bœuf et de taurillon n’évoluent pas avant l’âge de 2 ans. Avant 35 mois, elles n’évoluent que très rarement chez la femelle mais au-delà, un effet négatif de l’âge peut parfois être mis en évidence. Parmi les facteurs liés au mode de conduite, il apparaît que la gestation et le vêlage ne détériorent pas la tendreté de la viande et que l’activité physique est favorable à la tendreté des muscles locomoteurs sans influencer celle des muscles non locomoteurs. Concernant la conduite alimentaire, il faut retenir que la croissance compensatrice, l’augmentation du niveau alimentaire et de la durée de finition peuvent influencer favorablement la tendreté de la viande, mais ces effets positifs restent à confirmer. La nature de l’alimentation a également des effets limités sur la tendreté de la viande, variables selon les aliments étudiés et les conditions d’expérimentation.
Relations entre pratiques de finition et performances à l’abattage de vaches de réforme Simmental Française : intérêt du caractère mixte de la race
Authors: GAILLARD C. , INGRAND S. , BLANQUET J. , DEVEVEY C. , ROUX M.
Published: May 2020

L’objectif de ce travail a été d’étudier les performances bouchères de vaches de réforme de race Simmental Française à partir de données d’abattage, puis de les mettre en relation avec les modes de production des animaux appréhendés en amont à travers des enquêtes en élevage.
Une Analyse Factorielle Multiple (AFCM) réalisée sur les caractéristiques d’abattage de 2743 vaches de réforme issues de 122 élevages de trois départements (Côte d’Or, Haute-Marne, Jura) a permis de définir quatre profils de performances bouchères, en fonction du poids de carcasse et de la conformation.
Une enquête directe dans vingt-cinq élevages, choisis par stratification, a conduit à distinguer par AFCM cinq profils d’exploitations en fonction des pratiques mises en œuvre et des ressources alimentaires mobilisées particulièrement lors de la finition.
Le croisement entre ces deux typologies met en évidence une grande diversité dans la valorisation du caractère mixte de la race. La gamme des pratiques d’élevage observées, va du non-engraissement jusqu’au tout-engraissement des vaches de réforme avec des écarts de performances entre groupes extrêmes de 70 kg de poids de carcasse et de plus d’une classe de conformation. Les performances les plus élevées (carcasse de poids>= 380 kg, classée R- à R=) sont associées à une forte mobilisation de ressources alimentaires, notamment l’ensilage de maïs mais également à la mise en œuvre de pratiques spécifiques telles que le tarissement ou la prise en compte des critères de morphologie bouchère dans la sélection des vaches laitières.
Les liaisons observées entre pratiques d’élevage et performances révèlent donc différentes logiques de fonctionnement d’élevage mais aussi différentes perceptions des éleveurs concernant le type génétique recherché. Les combinaisons identifiées entre pratiques et niveau de performances semblent configurer un gradient d’expression de la mixité, avec la possibilité de déplacer un curseur situant le niveau d’équilibre lait-viande exprimé par l’éleveur en fonction de ses objectifs et de ses contraintes. Le réglage de ce curseur qui pourrait constituer un atout des races mixtes souligne toutefois la difficulté inhérente à l’élaboration de programmes de sélection, recherchant cet équilibre entre aptitudes laitières et bouchères.
Формирование паттернов в системе реакции-диффузии с дробными производными по времени
Authors: Зенюк Дмитрий Алексеевич , Zenyuk Dmitry Alexeyevich , Малинецкий Георгий Геннадьевич , Malinetskii Georgii Gennad'evich
Published: May 2020

На примере абстрактной модели брюсселятора рассмотрены сценарии формирования сложных паттернов в нелинейных средах с диффузией и дифференциальными операторами нецелого порядка. С помощью стандартной техники линейного анализа получены точные выражения для критических значений параметров, при которых в системе наблюдаются неустойчивости определенного типа. Для бифуркации Хопфа и бифуркации коразмерности 2 критерии устойчивости существенно зависят от порядка дробной производной. Предсказания линейной теории подкреплены результатами численного анализа.
Параллельный алгоритм моделирования течения в системах ротор-статор на основе рeберно-ориентированных схем
Authors: Абалакин Илья Владимирович , Abalakin Ilya Vladimirivich , Бахвалов Павел Алексеевич , Bakhvalov Pavel Alekseevich , Бобков Владимир Георгиевич , Bobkov Vladimir Georgievich , Горобец Андрей Владимирович , Gorobets Andrei Vladimirovich
Published: May 2020

Описывается численная методика расчeта динамики газа в системах ротор--статор на основе скользящих сеток и рeберно-ориентированных схем. Особое внимание уделяется параллельной реализации алгоритма с использованием комбинированного MPI+OpenMP распараллеливания для кластерных систем. Показана параллельная эффективность в расчетах с использованием до 1400 ядер, а также на ускорителях Intel Xeon Phi. Проводится верификация алгоритма на линейной акустической задаче. Работоспособность алгоритма демонстрируется на примере расчeта модельного вентилятора.
Kondisi Thermal Masjid Al-Fairus Pekalongan
Authors: Taqwim S M Ainut , Hardiman Gagoek , Budi Wahyu Setia
Published: May 2020

Alfairus Mosque is a mosque that has a design concept that adopts the design of the Nabawi mosque. The building that adopted the design of the Nabawi mosque in Medina has a characteristic high and wide openings on the outer facade with a combination of arch ornaments like most Persian mosque designs. This research focuses on the effect of openings and air movement in space which is a variable used to measure thermal comfort. In the implementation of this research, the existing measurements were carried out when there were no worship activities and measurements when there were worship activities. From the results of the study it was found that the temperature difference was very significant when data collection was in a condition without worship and there were worship activities. In data collection when empty conditions were taken at 10:00 WIB and at the time of worship activities were taken at the time of the Friday prayer service. When empty conditions are obtained the highest effective temperature is 27.80 ° C, while during worship services the highest effective temperature reaches 32.20 ° C. And at the highest temperature at each measuring point is the location of the center of the mosque where the location of the measuring point is far from the opening or hole of the mosque. While the lowest temperature is at the location close to the opening of the wall hole where the wind blows from that direction. In this study the lowest temperature was at the north side because at the time of the study the wind blew from the north to the south. Air movement greatly affects the decrease and increase in effective temperature in space, air movement is influenced by openings and wind direction. The higher the air movement the lower the effective temperature.

 
Implementasi Teori Waktu Dengung Pada Balai Musik Jazz di Surakarta Studi Kasus : Auditorium Dan Jazz Club
Authors: Audryn Talisa , Sumadyo Amin , Marlina Avi
Published: May 2020

Surakarta has high interest in the development of music such as Jazz music. This issue can be seen from the existence of the largest Jazz music community in Surakarta that actively organizes regular events named SoJazz Society. Surakarta also has an annual routine event named Solo City Jazz. However, all of Jazz music events in Surakarta do not have an appropriate place acoustically based on reverberation time. The purpose of this design is to implementing reverberation time in the interior design of the auditorium and jazz club of Jazz Music Hall in Surakarta. According to the planning location in Surakarta, there is a possibility of  collaboration between Jazz music and Central Javanese music because the city of Surakarta is famous for traditional performing arts. Beside that, Jazz music and Traditional music also have the same reverberation time range. The method used in this design is quantitative regarding to reverberation time. The result of this design are Auditorium and Jazz Club which are designed with the arrangement of centralized speaker and the application of Heradesign, Thermatex, Plasterboard, cloth, carpet and wood materials in this design. Auditorium designed with hexagonal shape, the distance between spectators and performers is not more than 40m.
Kajian Implementasi Desain Universal Pada Taman Samarendah
Authors: Noviana Mafazah , Hidayati Zakiah
Published: May 2020

City Parks in terms of social functions can be used as a place for social interaction, a means for sports, play and recreation. As a public facility, city parks must accommodate all groups of people, ranging from normal people, children, disabled people and the elderly. One way to provide facilities for all visitors is to apply universal design principles. Universal design aims to facilitate everyone's life through the creation of products, the built environment and communication to be used by as many people as possible and provide added value for everyone. The purpose of this study is to examine the implementation of universal design principles in Taman Samarendah.The location of the study was in Taman Samarendah, using a descriptive qualitative research method. Seven principles of universal design and Permen PUPR No.14/PRT/2017 becomes the guideline and standardization in this study. The results of this study indicate that Samarendah Park has not fully applied the universal design principles and accessibility standards. The most universal principles of design that are not applied are the principle of tolerance for error and the principle of low physical effort. The principle that is most widely applied is the size and space for approach.
Analisis Motor Ability Terhadap Keterampilan Teknik Permainan Futsal pada Anak Usia 11 Tahun
Authors: Sutirta Habibi , Abdul Karim Abdurrauf
Published: May 2020

The research is correlational and inferential, so it is necessary to design and examine the contribution of strength, speed, agility, and explosive power toward technical skill in futsal. The results of the research reveal that (1) there is significant contribution of strength towards students' Technical Skillin futsal at SD Rama Sejahtera Kecamatan Panakukang Kota Makassar by 50,8% proved from the result of analysis which obtained observation regression value (Ro) = 28,903 (p<0,05) with determination coeffiecient by 0,508. (2) there is significant contribution of speed towards students' technical skill in futsal at SD Rama Sejahtera Kecamatan Panakukang Kota Makassar by 38,8% proved from the result of analysis which obtained observation regression value (Ro) = 17,730 (p<0,05) with determination coeffiecient by 0,388. (3) there is significant contribution of agility towards students' technical skill in futsal at SD Rama Sejahtera Kecamatan Panakukang Kota Makassar by 28,0% proved from the result of analysis which obtained observation regression value (Ro) = 10,914 (p<0,05) with determination coeffiecient by 0,280. (4) there is significant contribution of explosive power towards students' technical skill in futsal at SD Rama Sejahtera Kecamatan Panakukang Kota Makassar by 62,3% proved from the result of analysis which obtained observation regression value (Ro) = 46,194 (p<0,05) with determination coeffiecient by 0,623. (5) there are strength, speed, agility, and explosive power contribution towards students' technical skill in futsal at SD Rama Sejahtera Kecamatan Panakukang Kota Makassar by 66,9% proved from the result of analysis which obtained observation regression value (Ro) = 12,619 (p<0,05) with determination coeffiecient by 0,699.
Hubungan Antara Tingkat Kepercayaan Diri Dengan Prestasi Belajar Pendidikan Jasmani
Authors: Mulya Gumilar , Agustryani Resty
Published: May 2020

This study aims to examine the level of confidence relationship with physical education learning achievement in elementary schools in the Tawang sub-district, Tasikmalaya City. The research method uses descriptive-correlational. The sample in this study is the sixth-grade students selected representing several schools in the District of Tawang as many as 60 students employing a purposive sampling technique. The instrument used was a confidential questionnaire. Data processing procedures to obtain answers about whether or not the hypothesis is accepted following the proposed significance are: (1) looking for averages, (2) looking for standard deviations, (3) testing normality through the Lillefors test (4) testing the correlation of two variables. Based on the results of research and statistical calculations on hypothesis testing the relationship of the level of confidence with physical education learning achievement, the authors can conclude as follows; there is a significant relationship between self-confidence and student achievement in class VI in Tawang District, Tasikmalaya City. Because the significance value obtained is greater than 0.05, and the value of Yxy (0.90)> (0.250) rtable (0.05) (60) then H0 is rejected and Ha is accepted, so there is a significant relationship between self-confidence and achievement learning (significant).
Interposition Arthroplasty of the Elbow After Proximal Ulna Reconstruction With the Masquelet Technique
Authors: Carroll John J. , Henderson Chance , Watson Jeff , Larsen Karl , Kobayashi Ky
Published: May 2020

Introduction:

Periarticular bone loss in the upper extremity poses challenges for a functional reconstruction.

Case:

A 32-year-old man sustained an open elbow fracture-dislocation from a high-velocity gunshot resulting in significant bone and soft-tissue loss about the proximal ulna. He underwent bony reconstruction with the Masquelet technique and delayed interposition arthroplasty in an attempt to preserve function.

Conclusions:

The Masquelet technique, coupled with interposition arthroplasty, may be used to address significant articular bony defects about the proximal ulna, allowing patients to maintain function and limit pain.
Shoulder Hemiarthroplasty After Previous Pectoralis Major Transfer for Irreparable Subscapularis Tear
Authors: Paclot Julien , Gasbarro Gregory , Kirsch Jacob M. , Neyton Lionel
Published: May 2020

Case:

A 30-year-old woman with an irreparable subscapularis tear presented with persistent painful anterior instability despite several open and arthroscopic stabilization procedures. A pectoralis major tendon transfer (PMTT) was performed. The patient subsequently developed progressive glenohumeral arthritis over the next 10 years, ultimately necessitating shoulder arthroplasty.

Conclusions:

PMTT provides valuable anterior soft-tissue reconstruction stabilization for subscapularis insufficiency in a multioperated shoulder. Ten years later, the transfer was found to be intact and managed like a native subscapularis during anatomic shoulder replacement, thus avoiding a reverse arthroplasty in a young patient.
A novel approach reveals genomic landscapes of single-strand DNA breaks with nucleotide resolution in human cells
Authors: Gao Fan , Cai Ye , Tang Lu , Wu Chun-Lin , Cao Huifen , Xu Dongyang , Salazar-García Lorena , Han Xueer , Wang Fang , Ricciardi Natalie , Wahlestedt Thor , Cai DingDing , Wang Huifang , Chin Mario P. S. , Timmons James A. , Wahlestedt Claes , Kapranov Philipp
Published: May 2020

Single strand breaks (SSBs) represent the major form of DNA damage, yet no technique exists to map these lesions genome-wide with nucleotide-level precision. Herein, we present a method, termed SSiNGLe, and demonstrate its utility to explore the distribution and dynamic changes of genome-wide SSBs in response to different biological and environmental stimuli. We validate SSiNGLe using two very distinct sequencing techniques and apply it to derive global profiles of SSBs in different biological states. Strikingly, we show that patterns of SSBs in the genome are non-random, specific to different biological states, enriched in regulatory elements, exons, introns, specific types of repeats and exhibit differential preference for the template strand between exons and introns. Furthermore, we show that breaks likely contribute to naturally occurring sequence variants. Finally, we demonstrate strong links between SSB patterns and age. Overall, SSiNGLe provides access to unexplored realm of cellular biology, not obtainable with current approaches.
Dental Caries Status among the Elderly in the Rural Areas of Yunnan Province, China: A Cross-Sectional Study
Authors: Zhang Shinan , Ding Hui , Shen Xun , Pu Lingli , Li Yingmei , Cai Le , Yao Lan
Published: May 2020

Background: Elder population is considered as a vulnerable group with high risk of dental caries. The older adults living in rural areas of Yunnan province account for approximately half of the province’s total population. However, few studies have addressed their dental caries status. The purpose of the present study was to investigate the dental caries status and their associated factors among 65-74 years old people in the rural areas of Yunnan province, China. Methods: This cross-sectional study recruited participants through a multistage and stratified strategy, and comprised a clinical oral examination and a questionnaire survey. Two trained and calibrated dentists, wearing LED headlights, conducted the clinical assessment with dental mirrors and CPI probes. Caries experience was measured using the DMFT and DF-Root indices. A face-to-face questionnaire survey was conducted to collect the participants’ socio-demographic information, oral health-related knowledge and practices. A multi-factor ANCOVA test was used to detect the factors influencing coronal and root caries. Results: 1175 elderly residents aged 65-74 were included. The majority of the surveyed elderly respondents (87%) experienced coronal caries (DMFT>0), and 56% of the respondents had root caries. Their overall mean DMFT and DF-root scores were 10.4±10.9 and 2.4±3.8, respectively. In total, 12% of the participants were edentulous. In the final model, female (β = 2.872, p < 0.001) and residents who living in less developed areas (Jianchuan district) (β = 9.601, p < 0.001) had higher mean DMFT scores. DF-Root scores were also higher among female (β = 0.739, p < 0.001), residents who living in less developed areas (Jianchuan district) (β = 2.082, p < 0.001). In addition, elderly who had lower annual family income were more likely to had more root caries (β = 0.642, p < 0.001) . Conclusion: The dental caries status among 65-to 74-year-old in the rural areas of Yunnan province, China, was poor. Their coronal and root caries experience both varied by gender and place of residence. Cost-effective prevention and treatment programs for caries management should be implemented to benefit the elderly population in the rural areas of Yunnan province.
The experience of nurses whose role requires allocating patients to outlier and over census beds during access-block: a review of the literature
Authors: Metcalfe Lauren-Leigh , Frost Steven Alfred , Christensen Martin
Published: May 2020

Background

Strategies intended to streamline ED patient admission and discharge have been introduced to alleviate access block. To meet policy, local hospitals design demand management processes which are intended to meet the changing needs of the facility by directing the patient journeys throughout the hospital. Given the crucial role patient flow managers and after-hours nurse managers play in managing patient flow there is a need to explore the experience of these nurse managers while managing access block.
Objectives

To gain a better understanding of the nurse managers experience of allocating patients to outlier or over-census beds.
Methods

A systematic literature search was undertaken and reported using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline. The search was for research papers which described the nurse manager’s experiences utilising outlier or surge beds at times of access block. The review included all relevant literature, and papers were not excluded based on study design or methodology.
Results

The electronic search identified 2,057 papers. The inclusion/exclusion criteria removed 2,010 papers, leaving 47 papers for further abstracts, or full text screening. Finally, five papers were identified for possible inclusion in the review. Two co-authors independently assessed the five, final papers for inclusion, yet, none contained the nurse managers experience of allocation patients to outlier beds.
Conclusion

The outlier and over-census protocols are two strategies employed in response to periods of peak demand. The application of these strategies is the responsibility of the nurse manager. Up until now, evidence remains unreported on the experience of nurse managers responsible for managing resources at times of access block. In an attempt to address this gap we have planned to address this as an area of future research.
Effects of IL-1β-induced ADAMTS-4/-5 and TAK1 on the degradation of cartilage aggrecan in the progression of human osteoarthritis
Authors: Zhang Qiqi , Xu Shenglin , Fang Yinglei , Hua Changcheng , Wang Xiaohe , Hu Yong
Published: May 2020

Objective

Interleukin-1β (IL-1β) is a potentially important cytokine involved in several pathological processes of osteoarthritis (OA). It is well known that a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-4/-5) and transforming growth factor-β activated kinase 1 (TAK1) contribute to the degradation of human OA cartilage aggrecan. The purpose of this study was to investigate the mechanism by which IL-1β induced the expression of ADAMTS-4/-5 and TAK1 in human OA cartilage aggrecan degradation.
Methods

The pathological changes of cartilage tissues and chondrocytes were observed by histomorphological analysis. OA chondrocytes were isolated from human articular cartilage tissues and stimulated with 10 ng/ml of IL-1β at the per-designed times (24 h, 48 h, 72 h). Expression of ADAMTS-4/-5 and TAK1 in cartilage tissues and chondrocytes were measured using immunohistochemistry (IHC) and Western blot (WB).
Results

The level of IL-1β in synovial fluid in the normal group was significantly lower than that in OA group. The expression of ADAMTS-4/-5 and TAK1 were simultaneously upregulated in human OA cartilage tissues,and ADAMTS-4 was more positively correlated with TAK1 than ADAMTS-5. Furthermore, the expression of ADAMTS-4/-5 and TAK1 were increased by stimulation with IL-1β in human OA chondrocytes, but no positive correlation was found.
Conclusion

It is proposed that reducing the expression of ADAMTS-4/-5 in prevention of OA may involve the inhibition of TAK1 activity.
Maintenance Efficacy of Low Dose Imiglucerase for Gaucher Disease
Authors: Qiu Zhengqing , Zhang Baohui , Song Yuhang , Zhang Hongbing , Wu Yuting
Published: May 2020

Background

Imiglucerase is the recommended treatment for Gaucher disease (GD), a hereditary metabolic disease. In high risk adults and all children, the minimum recommended dose for long-term maintenance is 30 U/kg/2 weeks. However, the extremely high cost of this enzyme largely hinders its clinical use. The minimal maintenance dose of imiglucerase has thus been a subject of debate. We aimed to analyze the long-term maintenance outcomes of imiglucerase at dosage < 20 U/kg/2 weeks after standard dose.
Methods

Seventeen patients with GD type 1 or GD type 3 were enrolled for analysis. We evaluated maintenance efficacy of imiglucerase on hemoglobin, platelet, visceral volumes and bone conditions during the 7-year follow-up.
Results

Parameters on hemoglobin, platelet, liver, and spleen volumes of all patients were stabilized or improved. Seven out of 14 patients showed bone mineral density improvement. Three out of 16 patients showed worse bone pain; 6 out of 15 patients showed worse Erlenmeyer flask; 6 out of 15 patients showed worse bone infarction; 1 out of 16 patients showed worse marrow infiltration and 3 out of 15 patients showed worse osteonecrosis.
Conclusions

Imiglucerase less than 20 U/kg/2 weeks is enough to maintain blood and visceral parameters, but is not sufficient to stabilize skeletal conditions.
Is Current Depressive Symptoms Associated with the Perception of Poor Neighborhood Quality in Childhood? A Population-based Study
Authors: Guo Jing , Zhu Yingxue , Fang Liming , Fu Mingqi , Li Min
Published: May 2020

Background Elderly depressive symptoms are an increasing important issue worldwide. Poor neighborhood quality in childhood may increase the risk of depressive symptoms in old age from the perspective of life span theory. The aims of this study were to examine the association between the perception of neighborhood quality during childhood and depressive symptoms in older age.Methods Data was taken from the China Health and Retirement Longitudinal Study (CHARLS), and a total of 7207 individuals aged 60 years or older were included. Robust multivariable linear regression analysis was applied to estimate the association between the perception of childhood community quality and depressive symptoms, and to examine the interaction effects of education and childhood community quality on depressive symptoms.Results This study suggested that individuals who perceived the childhood community as unsafe, deficient in close relationship, unclean demonstrated higher risk in suffering from depression. Furthermore, a significant gender difference has been found. However, no significant interaction effect of education revealed.Conclusion This study proposed that the perception of neighborhood quality during childhood is an important factor associated with depressive symptoms in old age. We urge that older adults’ mental health issues could be examined from a childhood neighborhood quality perspective, and call for further steps to promote neighborhood quality lived by Chinese citizens.
Modulation of Plasma Sphingosine-1-Phosphate Levels via Dietary Salt Intervention in Chinese Adults:an Intervention Trial
Authors: Ma Qiong , Chu Chao , Xue Yan-bo , Hu Jia-wen , Zheng Wen-ling , Yan Yu , Wang Ke-ke , Wang Yang , Yuan Yue , Liao Yue-yuan , Chen Chen , Mu Jianjun
Published: May 2020

Background Sphingosine-1-phosphate (S1P), a pleiotropic bioactive sphingolipid metabolite, is involved in various pathophysiological processes,including blood pressure regulation. Salt is a crucial factor for blood pressure modulation,especially in salt-sensitive individuals who may develop earlier, more severe subclinical target organ damage than salt-resistant individuals.However, the relationships among salt intake, circulating S1P levels, and blood pressure changes are unknown. Thus, we conducted this intervention trial to explore the effect of dietary salt intake on plasma S1P levels and examine the relationship between S1P and blood pressure in Chinese adults.Methods Forty-two participants (aged 18–65 years) were recruited from a rural community in Shaanxi, China. All participants first maintained their normal diet for 3 days, then sequentially ate a low-sodium diet (3.0 g/day NaCl) for 7 days, followed by a high-sodium diet (18.0 g/day NaCl) for 7 days. We assessed their plasma S1P concentrations on the last day of each intervention phase by liquid chromatography–tandem mass spectrometry. We classified the subjects who demonstrated at least a 10% increase in mean arterial pressure upon transitioning from a low-salt to a high-salt diet as salt-sensitive and the others as salt-resistant. Differences in repeated measures were analyzed by repeated-measures analysis of variance.Results Plasma S1P levels decreased significantly from the baseline to low-salt diet period and increased from the low-salt tohigh-salt diet period. We observed this response in both salt-sensitive and salt-resistant individuals. Plasma S1P levels positively correlated with 24-hour urinary sodium excretion, but not 24-hour urinary potassium excretion. In line with plasma S1P level responses to salt intervention, systolic blood pressure and mean arterial pressure decreased from the baseline to low-salt diet period and increased from the low-salt to high-salt period.Systolic blood pressure positively correlated with plasma S1P; the correlation was stronger in salt-sensitive individuals than in salt-resistant individuals.Conclusion Low-salt intervention decreased plasma S1P levels, whereas high-salt intervention reversed this changein Chinese adults. This finding provides evidence that salt moderation may be a high-efficiency, low-cost intervention for regulating circulating S1P levels, with implications for salt-induced blood pressure modulation.Trial registration:NCT02915315.Registered 27 September,2016,http://www.clinicaltrials.gov
Relationship between physical strength measurements and anthropometric measurements: examples from Santal labourer population of Birbhum District, West Bengal, India
Authors: MALAKAR BISWANATH , Roy Subrata K. , Pal Baidyanath
Published: May 2020

Background: Muscular/ physical strength are fundamental in order to satisfy basic needs for survival and productivity, especially among labourer population in developing countries. Research suggests physical body dimension is one of the key determinants of strength; however, the relationship between overall physical body dimension and strength is yet to explored. Aims: to explore the relationship between strength measurements and anthropometric profile of Santal labourer population.Subjects and methods: Cross sectional data on strength (handgrip and back strength) and anthropometric measurements were taken from 536 healthy adult Santal labourers (Male=251, Female=285) aged between 18 to 65 years of Birbhum district, West Bengal. Statistical analysis including Pearson’s correlation and canonical correlation analysis (CCA) were used. Results: Strength negatively correlated with age and positively correlated with most of anthropometric measurements. CCA between strength and anthropometry were 0.765 (p<0.001, explained 85.53% total variance) and 0.611 (p<0.001, explained 71.41% total variance) for males and females respectively. Cross-loading value of CCA indicate fat-free-mass, forearm circumference and weight for male and fat-free mass, chest circumference and wrist breadth for female were most important predictors of strength.Conclusion: Fat-free mass, forearm circumference, weight, chest circumference, wrist breadth were the key measurements for overall strength of Santal labourer population.
Midwives’ expectations from pregnant women regarding their participation in safe delivery: a qualitative study
Authors: Bayrami Roghieh , Baghaei Rahim
Published: May 2020

Background : Pregnant women’s participation in safe delivery is associated with safety assurance and improvement. Safety and care quality improvement in developed countries has so far been based on research and clinical evidence. Nonetheless, stakeholders’ preferences and expectations were not much taken into account. This study aimed to explore midwives’ expectations from pregnant women regarding their participation in safe delivery. Methods : This qualitative study was conducted in 2019 in three public hospitals in Urmia, Iran. Data were collected through 24 in-depth semi-structured interviews with 24 midwives. Sampling was done purposively and continued up to data saturation. Data were analyzed through Graneheim and Lundman’s qualitative content analysis. Results: Midwives’ expectations from pregnant women regarding their participation in safe delivery were grouped into the two main themes of effective communication (with two main categories) and empowerment of pregnant women (with three categories). The five main categories of these two themes were client’s interaction with midwife, confidence in midwife, readiness for delivery, health literacy improvement, and participation in safe behaviors, respectively. Conclusions : Understanding midwives’ expectations regarding pregnant women participation in safe delivery is important to the development of policies for care quality and safety improvement in perinatal care. Keywords : Patient participation, Safe delivery, Qualitative study
Where is the best cup position for Crowe III hips with the presence of a false acetabulum in total hip arthroplasty?
Authors: Shen Junmin , Sun Jingyang , Du Yinqiao , Ma Haiyang , Zhang Bohan , Zhou Yonggang
Published: May 2020

Background

We aimed to evaluate the clinical and radiological results of three different cup positions for Crowe III hips with a false acetabulum.
Methods

From January 2008 to December 2018, we retrospectively evaluated 61 hips (57 patients) which were classified as Crowe type III and presented a false acetabulum. Based on the position of cup on postoperative radiographs, all hips were divided into three groups: anatomic group (A group), false acetabular group (F group) and middle group (M group).
Results

There were no significant differences in comparison of anteversion, inclination and leg length discrepancy among three groups. The cup size of A group was significantly smaller than other two groups. In the A group, autograft was utilized in 5 hips (33.3%) and a S-ROM stem was used more frequently. By the comparison of horizontal distance, the F group showed an overmuch lateralization of rotation center. At the last follow-up, the mean Harris hip scores were significantly improved in three groups. There were 2 patients from the M group with both a moderate limp and positive Trendelenburg sign. During the follow-up period, one hip of the M group performed an acetabular revision by the reason of acetabular fracture.
Conclusions

All three cup positions for Crowe III hips with a false acetabulum showed good clinical outcomes and no significant difference. However, compared with the anatomical position, the mid-position allows for larger acetabular cups and liners. Further, the mid-position allows for more mediazation compared with the false acetabular position.
Long Non-coding RNA PTCSC3 Promotes Apoptosis of Human Papillary Thyroid Carcinomas by Regulating S100A4
Authors: Wu Qiong , Jiang Liang , Wu Jiang , Dong HaiFang , Zhao Yaping
Published: May 2020

Objective: We investigated the effect of papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) on the apoptosis of human papillary thyroid carcinomas cells by regulating S100A4.Methods: Normal cells Nthy-ori 3-1, cancer cells SW579 and TPC-1 were used to study. Proliferation of human papillary thyroid carcinomas cells was detected by CCK8, clone formation and transwell. Tube formation assay was used to observe the formation of in vitro tubes. Western blot was applied to detect the expressions of S100A4. After S100A4 overexpressed or down expressed, the above experiments were repeated. Tumor volume and weight was measured. Ki67 expression and tumor microvascular density (MVD) were detected by immunohistochemistry. Apoptosis of tissues was detected by TUNEL. Expression of apoptosis-related proteins, VEGF and MMP-9 were detected.Results: PTCSC3 overexpression and S100A4 lowexpression had significantly decreased proliferation, invasion, migration and tube formation of cancer cells. Meanwhil, the expression of Ki67 and MVD in tumor tissues were significantly decreased (p<0.05).Conclusion: Up-regulation of PTCSC3 can inhibit the expression of S10044, and promoted cell apoptosis and tube formation of papillary thyroid carcinomas cells.
Clinical-like cryotherapy in acute arthritis of the knee improves inflammation signs, pain, joint swelling and motor performance in mice
Authors: Castro Paula Aiello Tomé de Souza , Machanocker Dafiner Helen , Perez Raphael Sanches , Cunha Thiago Mattar , Barbosa Germana Medeiros , Cunha Jonathan Emanuel , Oliveira Francisco Fábio Bezerra de , Ramalho Fernando Silva , Russo Thiago Luiz , Cunha Fernando Queiroz , Salvini Tania de Fátima
Published: May 2020

Background

Assess the effect of clinical-like cryotherapy on inflammatory signs (in vivo neutrophil migration, cytokines and joint inflammation), pain, and joint swelling in mice with knee arthritis. Balance and motor coordination were also evaluated.
Methods

Young C57BL/6 mice were randomly divided into 3 groups (n = 10/group): control group: animals were not immunized with antigen-induced arthritis (AIA) and no intervention occurred; AIA group: animals immunized with methylated bovine serum albumin (mBSA) for knee arthritis; AIA + cryotherapy group: animals immunized with mBSA and submitted to cryotherapy. After 21days, the study was divided into 6-hour experimental analysis periods after injection-induced joint inflammation with mBSA (100 ug/joint) and a cryotherapy protocol was applied (ice pack, two 20-minute sessions). Number of synovial fluid neutrophils, cytokine levels, joint histology, pain, joint swelling and motor performance were also analyzed.
Results

Our results showed that the application of cryotherapy in mice with acute knee arthritis reduced inflammatory signs and improved the symptoms of pain and joint swelling, as well as motor coordination and balance in the animals.
Conclusions

Clinical-like cryotherapy used in the acute phase of arthritis reduced inflammatory signs and improved the symptoms of pain, joint swelling, motor coordination and balance in the mice.
A multi-lncRNA signature for suvival prediction in esophageal squamous cell carcinoma (ESCC) patients
Authors: zhang Lan , Li Pan , Xing Chenju , Zhu Di , Zhang Jianying , Wang Weiwei , Jiang Guozhong
Published: May 2020

Background: The aim of this study was to identify prognostic long non-coding RNAs (lncRNAs) and develop a multi-lncRNA signature for suvival prediction in esophageal squamous cell carcinoma (ESCC) patients.Methods: The clinical and gene expression data from 301 ESCC patients were downloaded, including a corhort used as training set from Gene Expression Omnibus database (GSE53624, n=119), another cohort of 98 paired ESCC tumor and normal tissues as test set and an independent validation cohort including 84 ESCC tissues. Survival analyses, Cox regression and Kaplan–Meier analysis were used.Results: we screened a prognostic marker of ESCC from the GSE53624 dataset and named it as the five-lncRNA signature including AC007179.1, MORF4L2-AS1, RP11-488I20.9, RP13-30A9.2, RP4-735C1.6, which could classify patients into high- and low-risk groups with significantly different survival(median survival: 1.75 years vs. 4.01 years, log rank P<0.05). Then test dataset and validation dataset confirmed that the five-lncRNA signature can determine the prognosis of ESCC patients. Predictive independence of the prognostic marker was proved by multivariable Cox regression analyses in the three datasets (P<0.05). In addition, the signature was found to be better than TNM stage in terms of prognosis.Conclusion: The five-lncRNA signature could be a good prognostic biomarker for ESCC patients and has important clinical value.
Repression of BET activity sensitizes oral squamous cell carcinoma to PARP inhibition via inhibiting DNA homologous recombination and immune inhibition
Authors: Liu Xiaojing , Zhang Fenghe , Zhang Xin
Published: May 2020

Background: To investigate the root cause of therapeutic resistance underlying oral squamous cell carcinoma (OSCC) to the PARP inhibitor olaparib and explore the combination with the BET inhibitor JQ1 to augment its treatment effects.Material and Methods: Cell viability were detected in the study. We also evaluated the expression of a series of factors through quantitative real-time PCR, immunofluorescence and western blot. The effect of combining JQ1 and olaparib in xenograft OSCC mouse model was also examined.Results: The sensitivity of Cal27 cells to olaparib was better than Scc25. Functional assays demonstrated that olaparib induced HR repair and upregulated PD-L1 expression, which results in drug resistance of OSCC to olaparib. Variations of these factors in the two cell lines may explain different sensitivity to olaparib. Moreover, the JQ1 BET inhibitor and olaparib synergistically exhibited anti-cancer effects in OSCC in vitro and in vivo and inhibited essential HR repair factors RAD51, BRCA1, and TOPBP1 through the ATR/CHK1 pathway and immune suppression mediated by the PD-L1 pathway.Conclusions: Elevated HR and PD-L1 are involved in resistance mechanisms of OSCC to olaparib, attenuating its anti-tumor effects. Our results suggest that the strong synergistic anti-cancer activity of combining olaparib with BET inhibitor in OSCC may be via suppression of the ATR/CHK1-mediated DNA damage response and PD-L1-related immune escape, indicating this combination strategy as a possible therapy in OSCC.
Thoughts on Higher Medical Education Under Major Public Health Emergencies: Thinking Ahead After COVID-19 Outbreak—A case-control study
Authors: Lin Wei , Chen Yan , Shi Songchang , Liang Jixing , Huang Huibin , Li Liantao , Cai Liangchun , Zong Liyao , Wang Nengying , Wen Junping , Chen Gang
Published: May 2020

Background: The spread of coronavirus disease 2019 (COVID-19) has posed great threat to the global population’s health and nearly every medical school throughout the world suspended classes as a precaution against the virus. The aim of this research is to explore the most suitable teaching and learning pattern in medical school during COVID-19 Outbreak. Methods: This was a case-control study which conducted in a medical school. We applied a new blended teaching model based on 5G network that combined team-based learning (TBL) and online interaction to the students before the outbreak and when universities responded to the COVID-19 outbreak by closing campuses, they shifted to different forms of distance learning. Fifty fourth-year medical students receiving the “5+3” pattern courses regarding internal medicine were enrolled in the study. A five-point Likert scale questionnaire that contains 20 items was used to compare the effect of the two kinds of teaching patterns. It evaluated the indicators of core competencies of students including professionalism, attitude towards learning, knowledge and learning skills, teamwork skills, motivation in learning, adaptability and acceptance of the courses and network environment.Results: According to the descriptive statistical analysis of the first part of the questionnaire (question 1-16), the average score of adaptability and acceptance of the courses is 2.60 less than 3, indicating that students are more adapted to other forms of distance learning during COVID-19 outbreak; the average score of the rest of the questions is greater than 3, indicating that blended teaching model based on 5G network is superior to other forms of distance learning. The number of male students who are inclined to the blended teaching model based on 5G network is 0.13 times as much as that of female students (95%CI:0.028~0.602, p=0.009).Conclusion: The results that medical education based on 5G network that combined team-based learning (TBL) and online interaction is a more suitable option to teach medical students.
Encephalatrophy and anti-N-methyl-D-aspartate receptor encephalitis
Authors: Lin Yinyao , Hu Mengyan , Li Haiyan , Liu Sanxin , Men Xuejiao , Shan Yilong , Tan Sha , Huang Xuehong , Lu Zhengqi , Zhang Bingjun
Published: May 2020

Objective: This study aims to find whether there are differences of clinical and laboratory features between anti-NMDAR encephalitis patients with and without brain atrophy. We also compare brain atrophy scale in anti-NMDAR positive patients with and without teratomas. Methods: Brain atrophy scales of 82 patients with anti-NMDAR encephalitis were measured with the median temporal lobe atrophy (MTA) scale and the global cortical atrophy (GCA) scale. They were divided into two groups by brain atrophy status. In addition, 48 female patients with anti-NMDAR encephalitis were divided into two groups with or without teratomas. Percentages of patients with brain atrophy were compared between these two groups. Results: 33 (40.2%) patients had brain atrophy. The GCA (+) group had higher percentage of patients with status epilepticus (p<0.001) than GCA (-) group and the MTA (+) group had higher percentage of patients with memory deficits (p < 0.001) than MTA (-) group. Higher percentages of severe infections were found in GCA(+) and MTA (+) groups than those in GCA(-) and MTA (-) groups (p=0.01 and p = 0.002, respectively). The percentage of patients needing ventilatory support was higher in MTA (+) group than that in MTA (-) group (p=0.015). The modified Rankin Scale (mRS) scores of GCA(+) and MTA (+) groups were significantly higher when compared with GCA(-) and MTA (-) groups both at day one (p<0.001 and p=0.001, respectively) and at discharge (p<0.001 and p = 0.02, respectively). Patients in GCA (+) group had higher median length of hospital stay than those in GCA (-) group (p<0.001). The median onset-to-MR time of GCA(+) group was higher than that of GCA(-) group (p=0.034). Percentage of patients with limited response to treatment was higher in GCA (+) group than that in GCA(-) group (p<0.001). The median 25 hydroxyvitamin D3 level was lower in the GCA (+) group than that in GCA(-) group (p=0.001). Anti-NMDAR positive female patients with ovarian teratomas had higher percentage of GCA(+) than thoes without ovarian teratomas (P=0.006). Conclusions: Brain atrophy are not uncommon in patients with anti-NMDAR encephalitis. MTA and GCA may be associated with severity and prognosis of anti-NMDAR encephalitis.
Nasopharyngeal Microbiota Profiling of SARS-CoV-2 Infected Patients
Authors: De Maio Flavio , Posteraro Brunella , Ponziani Francesca Romana , Cattani Paola , Gasbarrini Antonio , Sanguinetti Maurizio
Published: May 2020

We analyzed the bacterial communities of the nasopharynx in 40 SARS-CoV-2 infected and uninfected patients. All patients had a mild COVID-19 disease. We did not find statistically significant differences in either bacterial richness and diversity or composition. These findings suggest a nasopharyngeal microbiota at least early resilient to SARS-CoV-2 infection.