bluuebunny/crossref_metadata_2025 huggingface dataset:
Indirect Selection for Seed Yield in Sacha-Inchi (Plukenetia volubilis) in Brazil
Authors: Delgado Jhon Paul Mathews , Chaves Francisco Célio Maia , Lopes Ricardo , Meneses Carlos , Valente Magno Sávio Ferreira , Rodrigues Filipe Almendagna , Pasqual Moacir , Ramos Santiago Ferreyra , de Aguiar Ananda Virginia , Lopes Maria Teresa Gomes
Published: October 2022

Breeding programs for improvement of sacha-inchi, Plukenetia volubilis L., generally aim to select individuals with greater seed yield since there is a strong correlation between seed yield and oil production. However, the manual removal of seed husks for evaluating this trait is laborious and costly, thereby discouraging breeding efforts. Accordingly, the objective of the present study was to estimate gains from indirect selection of seed production in sacha-inchi progenies, focusing on maximizing efficiency in improvement programs. Genetic parameters along with direct and indirect selection gains were estimated for seed yield traits in 12 open-pollinated progenies. Strong genetic correlations were observed between total number of fruits (TNF), total weight of fruits (TWF), and total weight of seeds (TWS) per plant (r > 0.96). Notably, all three traits demonstrated high heritability (h2 > 0.81). Therefore, plants with high TNF and TWF (Cuzco, Dos de Mayo, Shanao, Aucaloma, and AM-7) can be used to indirectly select the genetic traits of higher seed yields (GS% = 23%). Genetic gain for dry seed production with a selection index of 42% was estimated at 23%, which corresponds to 118 kg·ha−1. Future sacha-inchi improvement programs can select progenies with high TNF and TWF to facilitate the selection of progenies with high TWS.
Shelf Life Prediction and Kinetics of Quality Changes in Pineapple (Ananas comosus) Varieties at Different Storage Temperatures
Authors: Mohd Ali Maimunah , Hashim Norhashila , Abd Aziz Samsuzana , Lasekan Ola
Published: October 2022

Shelf life estimation is an important factor to predict the freshness of fruits. This study aimed to investigate the shelf life and the changes in the physicochemical properties of three different pineapple varieties, namely MD2, Josapine, and Morris stored at 5, 10, and 25 °C. The effect of storage temperature on pineapple changes in total soluble solids, pH, moisture content, firmness, and colour was evaluated for 21 days of the storage period. It was revealed that different storage temperatures have a significant effect on the shelf life and quality of different pineapple varieties. The firmness and moisture content showed high regression coefficients, hence were used for the shelf life prediction of pineapple based on kinetic models. By using first-order kinetics, the coefficient of determination (R2) values for quality changes in pineapples ranged from 0.893 to 0.992. The results also demonstrated that the samples stored at 10 °C had the longest shelf life in relation to the changes in firmness and moisture content of the fruit. The findings indicated that shelf life estimation plays an important role to improve the quality preservation of fresh fruits and vegetables during storage.
Greater Sunlight Exposure during Early Fruit Development Increases Polyphenol Concentration, Soluble Solid Concentration, and Fruit Mass of Cider Apples
Authors: Karl Adam Duerr , Peck Gregory Michael
Published: October 2022

The environmental factors that influence cider apple fruit quality, particularly bitter and astringent polyphenols, are not well understood. Five experiments were conducted to investigate how sunlight affects fruit and juice quality. In three studies, shade cloth was placed over entire trees or individual branches at different phenological stages, durations, and opacities. Influence of canopy microclimate was investigated by harvesting fruit from different sections of the tree canopy. In a final study, opaque paper bags were placed over fruit three weeks after full bloom (WAFB) until harvest. Polyphenol concentrations increased rapidly during the first five WAFB and were diluted as fruit grew larger. At harvest, fruit from unshaded trees had 32% greater total polyphenol concentrations and were 11% larger than trees shaded 1–5 WAFB. Shading branches later in the growing season reduced yield but had a modest and inconsistent reduction on polyphenol concentrations. Juice from fruit harvested from the top of the tree canopy had 33% greater polyphenol concentrations and 14% greater soluble solid concentrations than juice from the interior of the canopy. Bagging fruit had inconsistent impacts on polyphenol concentrations. We hypothesize that there is a source sink relationship between carbohydrate availability and polyphenol synthesis in apple fruit during the early stages of fruit development when most polyphenols are produced. Additionally, greater carbohydrate availability in canopies with greater sunlight exposure resulted in larger fruit and improved juice quality from a cider making perspective.
Comparative Study on Genome Size and Phytochemical Profile of Three Potential Species of Acacia: Threatened and Endemic to Saudi Arabia
Authors: Khan Salim , Al-Qurainy Fahad , Al-hashimi Abdulrahman , Nadeem Mohammad , Tarroum Mohamed , Salih Abdalrhaman M. , Shaikhaldein Hassan O.
Published: October 2022

Acacias are widely distributed in tropical and subtropical regions of the world and have both economic as well as medicinal value. The estimation of genome size is very important as it changes due to the change in noncoding DNA sequence as well as genome duplication among organisms for their evolutionary aspects. Three potential species of the genus Acacia including Acacia etbaica, Acacia johnwoodii and Acacia origena, which are threatened and nearly endemic to Saudi Arabia, were collected. The present study was carried out to determine the genome size (2C DNA contents), total phenolic content (TPC), total flavonoid (TFC) and some bioactive compounds in these species for their comparison. The genome size ranged from 1.91 pg (A. etbaica) to 2.45 pg/2C (A. origena) among the Acacia species, which correspond to genome sizes 1843.15–2364.25 Mbp, respectively. The variation was observed in genome size within Acacia species as nuclei were extracted using different extraction buffers except for GB and MB01 buffers. The FTIR analysis revealed the presence of various functional groups in compounds that might be responsible for different types of phytochemicals in these Acacia species. Total flavonoid content (TFC) ranged from 0.647 (A. origena) to 1.084 mg QE/g DW (A. etbaica), whereas the total phenolic f content (TPC) ranged between 15.322 (A. origena) to 28.849 (A. johnwoodii) mg/g DW of GAE. HPLC analysis revealed the presence of quercetin 3-β-D-glucoside and luteolin 7-rutinoside in the leaves of all three Acacia species in considerable amounts, and these might have good health-promoting effects. This is our first study on genome size (2C DNA content) using flow cytometry and phytochemical profiling on these Acacias. Thus, estimated genome size and phytochemical study of these species could help to understand the biosynthesis of secondary metabolites under various genes and the evolutionary relationships among them.
Identification and Comparative Analysis of the Rosaceae RCI2 Gene Family and Characterization of the Cold Stress Response in Prunus mume
Authors: Yang Lichen , Li Ping , Qiu Like , Ahmad Sagheer , Wang Jia , Zheng Tangchun
Published: October 2022

Rare cold inducible 2 (RCI2) proteins are a group of low molecular weight proteins that widely exist in various tissues of plants and play crucial roles in plant growth and development and abiotic stress responses. Genome-wide identification and analysis of RCI2 have not been documented in Rosaceae plants. Therefore, we identified 23 RCI2 genes from seven Rosaceae plants, which were classified into three subfamilies. The RoRCI2 protein encodes a highly conserved domain of Pmp3. Three homologous PmRCI2s genes from Prunus mume were cloned and named PmRCI2-1, PmRCI2-2, and PmRCI2-3. The results of subcellular localization prediction showed that three PmRCI2s localized to membrane structures, and the abscisic acid response element were found to have the largest number in the promoter sequences of PmRCI2s. The results of quantitative real-time PCR (qRT-PCR) showed that PmRCI2-3 was significantly induced by low temperature and highly expressed in stems and buds during the endodormancy stage. Our study improves the understanding of the RCI2 family of Rosaceae plants regarding the cold responses and provides a theoretical basis for the cold-resistant breeding of P. mume.
Inheritance of Fruit Weight, Size and Organic Acid Content in a Distant Hybrid Population of Longan ‘Huanongzao’ and Lychee ‘Ziniangxi’
Authors: Wang Jiahui , Liu Liqin , Dong Jiahui , Zhu Lifei , Wang Yicheng , Hu Guibing , Shi Shengyou
Published: October 2022

The type and content of organic acids in fruits are important factors that determine the traits, such as the nutrition and flavor of fruits. Fruit weight and size are also very important economic characters in fruit breeding research. To investigate the inheritance patterns in longan, fruit organic acid contents, weight, and size were measured in the distant hybridization progenies of longan ‘Huanongzao’ and lychee ‘Ziniangxi’. The results revealed that the malic acid accumulation exhibited mid-parent heterosis, citric acid content showed a tendency towards depression, and oxalic acid, ketoglutaric acid, fumaric acid, gallic acid, and total acid contents exhibited transgressive inheritance. The inheritance patterns in fruit weight, length and diameter tended towards smaller and degraded. The coefficient of variation (CV) for the organic acids ranged from 35.77–90.20%, and the heritability (H2) was 72.35–97.44%. High CV (55.44%) coupled with medium H2 (28.72%) were observed in fruit weight, however, low CV (12.29% and 12.50%) coupled with high H2 (68.95% and 69.25%) were shown in fruit length and diameter. In this study, the genetic variation of acid contents, fruit weight and size in the F1 population of longan and lychee were analyzed for the first time, providing an informative and theoretical basis for the selection and breeding of longan cultivars.
Quantification of Procedure Time and Infant Distress Produced (as Crying) When Percutaneous Achilles Tenotomy Is Performed under Topical Local Anaesthesia: A Preliminary Study
Authors: Vinyals Rodriguez Marta , Ey Batlle Anna , Jordan Iolanda , Míguez González Paula
Published: October 2022

Introduction: Percutaneous tenotomy of the Achilles tendon is part of the clubfoot management procedure known as the Ponseti method and is necessary for most infants requiring this treatment. However, the need to apply general anaesthesia or sedation during this procedure remains controversial. To our knowledge, no previous studies have been conducted to quantify infant distress, expressed as crying, when tenotomy is performed under local anaesthesia. Material and Methods: This clinical, prospective, cross-sectional, and observational study was composed of infants subjected to percutaneous Achilles tenotomy with local anaesthesia at an outpatient clinic. The degree of distress was measured using two smartphone apps (voice recorder and timer) in two iPhones, with each apparatus placed one meter from the baby. The following parameters were determined: procedure duration, crying duration, average crying intensity and maximum crying intensity. In addition, the following data were obtained: age, complications (if any) and the caregiver’s satisfaction with the process. Results: Among the 85 infants submitted to percutaneous tenotomy, the mean age was 1.95 (+/−1.632) months (ranging from 0 to 7 months), the mean duration of the procedure was 8.134 (+/−5.97) seconds, (range 2.1 to 33.5 s), the infants’ mean crying intensity was 88.99 dB and the maximum crying intensity was 96.56 dB. No vascular or anaesthetic-related complications were recorded. 96% of the caregivers were absolutely satisfied with the process. Conclusions: Percutaneous Achilles tenotomy performed under local anaesthesia can safely be performed at the outpatient clinic. The procedure is fast and the crying time and intensity (mean values: 84 s and 89 dB, respectively) are minimal and tolerable. Knowledge of these parameters provides more accurate knowledge about the procedure. The caregivers consulted were absolutely satisfied with the tenotomy performed under local anaesthesia. In future studies, these parameters can be used for comparison with related surgical approaches.
A Framework for Interfacing and Partnering with Environmental Justice Communities as a Prelude to Human Health and Hazard Identification in the Vulnerable Census Tracts of Columbus, Ohio
Authors: Lochotzki Heather , Williams Karen Patricia , Colen Cynthia G. , Adetona Olorunfemi , Tavares Charleta B. , Ginn Georgina M. , Haynes Rejeana , Im Wansoo , Bils Tanya , Hood Darryl B.
Published: October 2022

Columbus, Ohio is one of the more prosperous, well-educated, and progressive cities in the United States. However, it ranks as the second worst life expectancy at birth, has a census tract wealth gap (27-year disparity), and one of the higher infant mortality rates in the country. These data suggest that there are likely several high-risk, vulnerable neighborhoods in Columbus with residents experiencing disparate and adverse outcomes. Illustrative of this fact are studies that have examined the social processes and mechanisms through which neighborhood contexts are at the forefront, including exposures to chemical stressors such as particulate matter (PM2.5) as well as non-chemical stressors including violence, social determinants of health, zoning, and land use policies. It is documented that disparate and adverse outcomes are magnified in the vulnerable neighborhoods on the Near East Side as compared to Columbus city proper, Franklin County and/or the state of Ohio. As such, we developed a nuanced community engagement framework to identify potential environmental hazards associated with adverse pregnancy outcomes in those census tracts. The refined framework uses a blended version of traditional community-based participatory research (CBPR) models and is referred to as E6, Enhancing Environmental Endeavors via e-Equity, Education, and Empowerment.
Oral Health Knowledge, Attitudes and Practices of People Living with Diabetes in South Asia: A Scoping Review
Authors: Poudel Prakash , Rawal Lal B. , Kong Ariana , Yadav Uday N. , Sousa Mariana S. , Karmacharya Biraj , Pradhan Shaili , George Ajesh
Published: October 2022

Diabetes increases the risk of oral health complications. This review aimed to synthesise the current evidence on the oral health knowledge, attitudes and practices of people living with diabetes in South Asian countries and provide recommendations on possible strategies for addressing the gaps in oral health care in this population, including the role of non-dental professionals. Using a scoping review framework, six electronic databases (Ovid Medline, CINAHL, ProQuest Central, Scopus, Web of Science and Embase) were searched to identify the relevant literature published between January 2000 and December 2021. The data were extracted into three main categories based on the review’s aims, and further refined into sub-categories. A total of 23 studies were included. The review identified that while people with diabetes living in South Asian countries had some level of awareness about oral health and limited care practices to maintain good oral health, there were gaps in knowledge, and there were areas where their oral health practices and attitudes could be improved. The findings suggest a need for developing targeted oral health policies as well as implementing integrated oral health care interventions involving non-dental professionals to improve the oral health outcomes of people with diabetes.
Stress Levels, Attitude toward Vaccination and Personal Protective Equipment of Students at Wroclaw Medical University during the COVID-19 Pandemic
Authors: Kosendiak Aureliusz Andrzej , Adamczak Bartosz , Bania Julia , Kontek Sylwiusz
Published: October 2022

The study was conducted in March of 2021 on the students at Wroclaw Medical University from different years and faculties. Students who had mandatory physical education classes in the current year and met other eligibility criteria were enrolled. The aim of the study was to examine the impact of the pandemic on the lives of medical students. Ultimately, 660 responses to the study were included. To determine the level of stress, KPS questionnaires were used, which distinguish five types of stress. Moreover, the attitudes towards and status of vaccination were examined using PPE and declarative fettle. The findings pointed to a significantly higher level of stress in males compared to females (overall stress, 5.35 and 4.66, p = 0.0002), and increased external stress in some faculties (5.71 in dentistry, compared to overall 4.83, p = 0.009). Furthermore, medical students in their 2nd year were more stressed compared to those in their 1st year (overall stress 4.95 and 4.15, p < 0.0001). By knowing where the stress is highest, we can fight it more effectively and efficiently, by directing resources right where they are needed the most. A study about stress levels during the pandemic compared to physical activity should be developed.
WtsWrng Interim Comparative Effectiveness Evaluation and Description of the Challenges to Develop, Assess, and Introduce This Novel Digital Application in a Traditional Health System
Authors: Sampietro-Colom Laura , Fernandez-Barcelo Carla , Abbas Ismail , Valdasquin Blanca , Rabasseda Nicolau , García-Lorenzo Borja , Sanchez Miquel , Sans Mireia , Garcia Noemi , Granados Alicia
Published: October 2022

Science and technology have evolved quickly during the two decades of the 21st century, but healthcare systems are grounded in last century’s structure and processes. Changes in the way health care is provided are demanded; digital transformation is a key driver making healthcare systems more accessible, agile, efficient, and citizen-centered. Nevertheless, the way healthcare systems function challenges the development (Innovation + Development and regulatory requirements), assessment (methodological guidance weaknesses), and adoption of digital applications (DAs). WtsWrng (WW), an innovative DA which uses images to interact with citizens for symptom triage and monitoring, is used as an example to show the challenges faced in its development and clinical validation and how these are being overcome. To prove WW’s value from inception, novel approaches for evidence generation that allows for an agile and patient-centered development have been applied. Early scientific advice from NICE (UK) was sought for study design, an iterative development and interim analysis was performed, and different statistical parameters (Kappa, B statistic) were explored to face development and assessment challenges. WW triage accuracy at cutoff time ranged from 0.62 to 0.94 for the most frequent symptoms attending the Emergency Department (ED), with the observed concordance for the 12 most frequent diagnostics at hospital discharge fluctuating between 0.4 to 0.97; 8 of the diagnostics had a concordance greater than 0.8. This experience should provoke reflective thinking for DA developers, digital health scientists, regulators, health technology assessors, and payers.
Health Benefits Quantification for New-Energy Vehicles Promotion: A Case Study of Beijing
Authors: Wang Yue , Wen Yang , Xu Yingying , Shi Lei , Yang Xuan
Published: October 2022

Considering that the promotion of new-energy vehicles (NEVs) is a potential measure to address urban air pollution, the Chinese government has launched subsidy schemes to improve its market penetration. The purpose of this study is to quantify the economic benefits of NEV promotion from 2016 to 2019 and compare them with the cost of government subsidies in Beijing, so the effectiveness of the NEV policies can be evaluated and valuable recommendations can be provided for decision-making. The exposure–response model and the cost of illness approach was applied to evaluate the health and economic benefits of NEV promotion. Our results are as follows: (1) promoting NEVs can reduce the PM2.5 concentration significantly, the average annual concentration reduction was between 3.23 μg/m3 and 4.61 μg/m3; (2) at least 37,545 illnesses and deaths in Beijing could be reduced through NEV promotion annually, internal disease (15–64) was the most affected illness; (3) the economic benefits of NEV promotion were far more than the cost and the net benefits stock reached 33.71 billion CNY in 2019, accounting for 0.95% of Beijing’s GDP in the same period. This study provides references in the perspective of environmental economics for the policy formulation of promoting NEVs.
Can Professionalization Alleviate Job Burnout in Construction Workers in China? A Multivariable Mediating Model
Authors: Ni Guodong , Miao Xinyue , Li Li , Li Huaikun , Wang Shaobo , Niu Miaomiao
Published: October 2022

Burnout is at all-time highs across modern professions. As a typical labor-intensive industry, the high-pressure and task-driven nature of the construction industry makes construction workers more prone to burnout. It is still unclear whether increasing the professionalization level can lessen the many harmful consequences of job burnout on construction workers’ employment. Therefore, this study examined the influencing mechanism of professionalization on job burnout in the construction industry. First, a theoretical model based on the conservation of resources (COR) theory was developed with workload, job insecurity, and work–family conflict as moderating variables. A reliable sample of 441 Chinese construction workers were then recruited in the investigation. The data analysis was supported by confirmatory factor analysis (CFA) and structural equation modeling (SEM). The results indicated that: (i) an increase in the professionalization level could be directly effective in alleviating job burnout among construction workers; (ii) workload and work–family conflict could play an independent and continuous mediating role between professionalization and job burnout; and (iii) while job insecurity caused by a low professionalization did not have a direct impact on job burnout, it could have an indirect impact on job burnout through workload and work–family conflict, respectively. This study enriches the literature on job burnout among construction workers, as well as provides a theoretical basis and practical management guidance for Chinese construction companies to alleviate job burnout in workers from a professionalization standpoint.
The Waning of BNT162b2 Vaccine Effectiveness for SARS-CoV-2 Infection Prevention over Time: A Test-Negative Study in Health Care Professionals of a Health Department from January 2021 to December 2021
Authors: Jiménez-Sepúlveda Natali , Chico-Sánchez Pablo , Castro-García José Miguel , Escribano-Cañadas Isabel , Merino-Lucas Esperanza , Ronda-Pérez Elena , Sánchez-Payá José , Gras-Valentí Paula ,   on behalf of the COVID-19 Preventive Medicine Group
Published: October 2022

The duration of protection of vaccines against SARS-CoV-2 infection has been evaluated in previous studies, but uncertainty remains about the persistence of effectiveness over time and the ideal timing for booster doses. Therefore, the aim of this study was to evaluate BNT162b2 vaccine effectiveness against SARS-CoV-2 infection in health care workers (HCWs) at a tertiary hospital depending on time elapsed since the completion of a two-dose vaccination regimen. We conducted a case–control with negative test study between 25 January and 12 December 2021 that included 1404 HCWs who underwent an active infection diagnostic test (AIDT) to rule out SARS-CoV-2 infection due to COVID-19 suspicion or prior close contact with patients diagnosed with COVID-19. The adjusted vaccine effectiveness (aVE) for the prevention of SARS-CoV-2 infection 12 to 120 days after completing the full two-dose vaccination regimen was 91.9%. Then, aVE decreased to 63.7% between 121 to 240 days after completing the full two-dose regimen and to 37.2% after 241 days since the second dose. Vaccination against SARS-CoV-2 infection in HCWs remains highly effective after 12 to 120 days have elapsed since the administration of two doses of the BNT162b2 vaccine; however, effectiveness decreases as time elapses since its administration.
Remote, Whole-Body Interval Training Improves Muscular Endurance and Cardiac Autonomic Control in Young Adults
Authors: García-Suárez Patricia Concepción , Canton-Martínez Ermilo , Rentería Iván , Moura Antunes Barbara , Machado-Parra Juan Pablo , Aburto-Corona Jorge Alberto , Gómez-Miranda Luis Mario , Jiménez-Maldonado Alberto
Published: October 2022

High-intensity interval training (HIIT) is an exercise modality acknowledged to maintain physical fitness with more engagement in an active lifestyle compared with other traditional exercise models. Nevertheless, its effects on cardiac control and physical performance in an online-guided setting are not yet clarified. The present work assessed physical fitness and heart rate variability (HRV) before and after an online, home-based HIIT program in college-age students while pandemic lockdowns were in effect. Twenty university students (age: 21.9 ± 2.4 years.) that were solely enrolled in online classes were distributed into three groups: control—CON-(n = 6), 14 min of HIIT—HIIT-14-(n = 8), and 21 min of HIIT—HIIT-21-(n = 6). A maximal push-up test was employed to assess muscular endurance and performance, and resting HRV signals were collected with wireless heart rate monitors and were processed in Kubios HRV Std. (Kubios Oy, Finland). There was an increase in total push-up capacity compared to CON (p < 0.05 HIIT-21 vs. CON; p < 0.001 HIIT-14 vs. CON) after 8 weeks. A significant interaction was observed in high-frequency and low-frequency spectra ratios after the HIIT-21 intervention (p < 0.05). The current work demonstrated that either short- or mid-volume online, whole-body HIIT improves muscle strength, whereas mid-volume HIIT (HIIT-21) was the only intervention that developed a sympathovagal adaptation. This study showed promising results on muscular endurance and cardiac autonomic modulation through whole-body HIIT practice at home.
Vegetation Changing Patterns and Its Sensitivity to Climate Variability across Seven Major Watersheds in China
Authors: Wang Qin , Ju Qin , Wang Yueyang , Shao Quanxi , Zhang Rongrong , Liu Yanli , Hao Zhenchun
Published: October 2022

Climate changes have profound impacts on vegetation and further alter hydrological processes through transpiration, interception, and evaporation. This study investigated vegetation’s changing patterns and its sensitivity to climate variability across seven major watersheds in China based on a hybrid regionalization approach and a novel, empirical index—Vegetation Sensitivity Index (VSI). Vegetation showed linearly increasing trends in most of the seven watersheds, while decreases in vegetation were mostly found in the source regions of the Yangtze River Basin (YZRB) and Yellow River Basin (YRB), the forest and grassland areas of the Songhua River Basin (SHRB) and Liao River Basin (LRB), the Yangtze River Delta, and the Pearl River Delta during the growing season. The selected watersheds can be categorized into 11 sub-regions, and the regionalization result was consistent with the topography and vegetation types; the characteristics of vegetation dynamics were more homogeneous among sub-regions. Vegetation types such as forests and shrubland in the central parts of the YZRB were relatively more vulnerable to climate variations than the grasslands and alpine meadows and tundra (AMT) in the source regions of the YZRB and YRB and the Loess Plateau of the YRB. In arid and semi-arid regions, precipitation had a profound impact on vegetation, while, at low latitudes, solar radiation was the main controlling factor. Such comprehensive investigations of the vegetation–climate relationship patterns across various watersheds are expected to provide a foundation for the exploration of future climate change impacts on ecosystems at the watershed scale.
A Review of Polychlorinated Biphenyls (PCBs) Pollution in the Air: Where and How Much Are We Exposed to?
Authors: Othman Naffisah , Ismail Zaliha , Selamat Mohamad Ikhsan , Sheikh Abdul Kadir Siti Hamimah , Shibraumalisi Nur Amirah
Published: October 2022

Polychlorinated biphenyls (PCBs) were widely used in industrial and commercial applications, until they were banned in the late 1970s as a result of their significant environmental pollution. PCBs in the environment gained scientific interest because of their persistence and the potential threats they pose to humans. Traditionally, human exposure to PCBs was linked to dietary ingestion. Inhalational exposure to these contaminants is often overlooked. This review discusses the occurrence and distribution of PCBs in environmental matrices and their associated health impacts. Severe PCB contamination levels have been reported in e-waste recycling areas. The occurrence of high PCB levels, notably in urban and industrial areas, might result from extensive PCB use and intensive human activity. Furthermore, PCB contamination in the indoor environment is ten-fold higher than outdoors, which may present expose risk for humans through the inhalation of contaminated air or through the ingestion of dust. In such settings, the inhalation route may contribute significantly to PCB exposure. The data on human health effects due to PCB inhalation are scarce. More epidemiological studies should be performed to investigate the inhalation dose and response mechanism and to evaluate the health risks. Further studies should also evaluate the health impact of prolonged low-concentration PCB exposure.
Unraveling the Role of Objective Food Environment in Chinese Elderly’s Diet-Related Diseases Epidemic: Considering Both Healthy Food Accessibility and Diversity
Authors: Zhang Zhaohua , Luo Yuxi , Zhang Zhao , Robinson Derrick , Wang Xin
Published: October 2022

The essential role of the objective food environment in achieving healthy aging has been widely recognized worldwide. However, the existing empirical evidence is mostly based on Western cases, and how the objective food environment associates with health outcomes among Chinese elderly remains poorly understood. By merging nationally representative micro survey data with Baidu-based spatial data on the location of food outlets, this study develops accessibility and diversity indicators to explore the relationship between food environment and diet-related diseases among Chinese elderly and investigates how healthy lifestyles moderate this relationship. The results show that improvement in healthy food accessibility and diversity decreases both the probability and the number of diet-related diseases that the elderly suffer. Having more healthy lifestyle factors is associated with a lower risk of suffering from diet-related diseases and strengthens the negative effect of healthy food environment on suffered diet-related diseases. Heterogeneity effect analysis suggests that the relationship between objective food environment and diet-related diseases differs by city scale and income level. The findings of this study shed light on designing tailor-made policies for non-Western countries to promote healthy aging.
Associations of Gait Speed, Cadence, Gait Stability Ratio, and Body Balance with Falls in Older Adults
Authors: Nascimento Marcelo de Maio , Gouveia Élvio Rúbio , Gouveia Bruna R. , Marques Adilson , Martins Francisco , Przednowek Krzysztof , França Cíntia , Peralta Miguel , Ihle Andreas
Published: October 2022

To investigate the association between gait speed (GS), cadence (CAD), gait stability ratio (GSR), and body balance (BB) with falls in a large sample of older adults. The analysis included 619 individuals—305 men and 314 women (69.50 ± 5.62 years)—residing in the Autonomous Region of Madeira, Portugal. Mobility in GS, CAD, and GSR was assessed using the 50-foot walk test and BB by the Fullerton Advanced Balance scale. The frequency of falls was obtained by self-report. Linear regression analysis showed that higher performance in GS and BB was able to reduce the risk of falling by up to 0.34 and 0.44 times, respectively. An increase in the GSR value enhanced the risk of falling by up to 0.10 times. Multinomial analysis indicated that, in relation to the highest tertile (reference), older adults classified with GS and BB performance in the lowest tertile (lowest) had an increased chance (OR) of falling by up to 149.3% and 48.8%, respectively. Moreover, in relation to the highest tertile, the performance of the GSR classified in the lowest and medium tercile showed an increase in the chance of falling by up to 57.4% and 56.4%, respectively.
“I Would Rather Take the Vaccine Than Undergo Weekly Testing”: Correlates of Health Workers’ Support for COVID-19 Vaccine Mandates
Authors: Iliyasu Zubairu , Garba Rayyan M. , Aliyu Mansur A. , Gajida Auwalu U. , Amole Taiwo G. , Umar Amina A. , Abdullahi Hadiza M. , Tsiga-Ahmed Fatimah I. , Kwaku Aminatu A. , Kowalski Meira S. , Salihu Hamisu M. , Aliyu Muktar H.
Published: October 2022

This study examined the support for vaccine mandates and uptake among clinical and non-clinical staff at a tertiary hospital in northern Nigeria, focusing on variation of survey responses based on job position, socio-demographic characteristics, and perceived risk of infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Using an explanatory, sequential, mixed-methods design and deploying a pragmatic paradigm, 370 healthcare workers were administered structured questionnaires. This was followed by in-depth interviews with a sub-sample of respondents to further clarify the responses regarding support for the coronavirus disease 2019 (COVID-19) vaccine mandate. Findings demonstrated that less than one-half of respondents supported the COVID-19 mandate, and only one in three had received the recommended COVID-19 vaccine doses. Support for the vaccine mandate and vaccine uptake were predicted by profession, work experience, number of children, health status, and risk perception. Support for the vaccine mandate was ascribed to ethical and professional duty, whereas opposition was associated with respect for autonomy and human rights. This study documents the need to enhance support for vaccine mandates and uptake among healthcare workers through sustainable strategies, as Nigeria’s healthcare workers are considered a source of trust and role models for the rest of society.
Transcriptomic Responses to Chilling Reveal Potential Chilling Tolerance Mechanisms in Cucumber
Authors: Wang Xiang , Mi Shuang , Miao Huaiqi
Published: October 2022

Chilling is a devastating stress that has led to a crisis of production for cucumber (Cucumis sativus L.). To determine the molecular mechanisms underlying chilling responses in cucumber, we investigated physiological changes and transcriptomic responses to chilling stress in the chilling-tolerant inbred line CC and chilling-susceptible inbred line R1461. Physiological analysis showed that CC had a higher survival rate, lower H2O2 accumulation, and ion leakage than R1461 after chilling treatment. RNA-seq analysis identified 938 differentially expressed genes (DEGs) in response to chilling and revealed that chilling stress regulated the transcript levels of genes related to hormones, including auxin, salicylic acid (SA), jasmonic acid (JA), and ethylene. RT-qPCR and pharmacological analysis suggested that cucumber chilling tolerance was associated with variation in the gene expression involved in ethylene biosynthesis and signaling. Exogenously applying 1-aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene, improved the chilling tolerance of cucumber, while the exogenous application of the ethylene inhibitor AgNO3 impaired the chilling tolerance of cucumber. After ACC treatment, the difference in chilling tolerance between CC and R1461 disappeared, suggesting that the different chilling tolerance level between CC and R1461 is dependent on the ethylene biosynthesis and signaling pathway. In addition, a comparison of cucumber lines with different chilling tolerances revealed that chilling tolerance is highly associated with the up-regulation of C-repeat binding factor (CBF) genes, while natural variation in the promoter of CsCBF1 is associated with chilling response. This study thus provides information on transcriptomic responses in different varieties of chilling-tolerant cucumber and reveals potential chilling tolerance mechanisms that could be used to improve chilling tolerance in cucumber.
Costimulatory CD226 Signaling Regulates Proliferation of Memory-like NK Cells in Healthy Individuals with Latent Mycobacterium tuberculosis Infection
Authors: Murillo Oscar , Moreira Josimar Dornelas , Kujur Weshely , Velasco-Alzate Karen , Sen Santara Sumit , Konduru Nagarjun V. , Mulik Sachin
Published: October 2022

It is now widely accepted that NK cells can acquire memory, and this makes them more effective to protect against some pathogens. Prior reports indicate memory-like NK cells (mlNKs) in murine model of Mycobacterium tuberculosis (Mtb) as well as in healthy individuals with latent TB infection (LTBI). The increased expression of CD226 was evident in mlNKs from LTBI+ people after stimulation with γ-irradiated Mtb (γ-Mtb). We thus evaluated the contribution of costimulatory CD226 signaling in the functionality of mlNKs in LTBI+ people. We found that blockade of CD226 signaling using the antibody- or CRISPR/Cas9-mediated deletion of the CD226 gene in NK cells diminished the proliferation of mlNKs from LTBI+ people. Blocking CD226 signaling also reduced the phosphorylation of FOXO1 and cMyc expression. Additionally, cMyc inhibition using a chemical inhibitor reduced proliferation by mlNKs from LTBI+ people. Moreover, blocking CD226 signaling reduced glycolysis in NK cells, and the inhibition of glycolysis led to reduced effector function of mlNKs from LTBI+ people. Overall, our results provide a role for CD226 signaling in mlNK responses to Mtb.
Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific Oyster Crassostrea gigas
Authors: Jin Qianqian , Huo Chuncao , Yang Wenhao , Jin Kaidi , Cai Shuai , Zheng Yanxin , Huang Baoyu , Wei Lei , Zhang Meiwei , Han Yijing , Zhang Xuekai , Liu Yaqiong , Wang Xiaotong
Published: October 2022

Retinoic acid (RA) plays important roles in various biological processes in animals. RA signaling is mediated by two types of nuclear receptors, namely retinoic acid receptor (RAR) and retinoid x receptor (RXR), which regulate gene expression by binding to retinoic acid response elements (RAREs) in the promoters of target genes. Here, we explored the effect of all-trans retinoic acid (ATRA) on the Pacific oyster Crassostera gigas at the transcriptome level. A total of 586 differentially expressed genes (DEGs) were identified in C. gigas upon ATRA treatment, with 309 upregulated and 277 downregulated genes. Bioinformatic analysis revealed that ATRA affects the development, metabolism, reproduction, and immunity of C. gigas. Four tyrosinase genes, including Tyr-6 (LOC105331209), Tyr-9 (LOC105346503), Tyr-20 (LOC105330910), and Tyr-12 (LOC105320007), were upregulated by ATRA according to the transcriptome data and these results were verified by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. In addition, increased expression of Tyr (a melanin-related TYR gene in C. gigas) and Tyr-2 were detected after ATRA treatment. The yeast one-hybrid assay revealed the DNA-binding activity of the RA receptors CgRAR and CgRXR, and the interaction of CgRAR with RARE present in the Tyr-2 promoter. These results provide evidence for the further studies on the role of ATRA and the mechanism of RA receptors in mollusks.
Intraoperative Tumor Detection Using Pafolacianine
Authors: Dindere Mihaela Elisabeta , Tanca Antoanela , Rusu Mihaela , Liehn Elisa Anamaria , Bucur Octavian
Published: October 2022

Cancer is a leading cause of death worldwide, with increasing numbers of new cases each year. For the vast majority of cancer patients, surgery is the most effective procedure for the complete removal of the malignant tissue. However, relapse due to the incomplete resection of the tumor occurs very often, as the surgeon must rely primarily on visual and tactile feedback. Intraoperative near-infrared imaging with pafolacianine is a newly developed technology designed for cancer detection during surgery, which has been proven to show excellent results in terms of safety and efficacy. Therefore, pafolacianine was approved by the U.S. Food and Drug Administration (FDA) on 29 November 2021, as an additional approach that can be used to identify malignant lesions and to ensure the total resection of the tumors in ovarian cancer patients. Currently, various studies have demonstrated the positive effects of pafolacianine’s use in a wide variety of other malignancies, with promising results expected in further research. This review focuses on the applications of the FDA-approved pafolacianine for the accurate intraoperative detection of malignant tissues. The cancer-targeting fluorescent ligands can shift the paradigm of surgical oncology by enabling the visualization of cancer lesions that are difficult to detect by inspection or palpation. The enhanced detection and removal of hard-to-detect cancer tissues during surgery will lead to remarkable outcomes for cancer patients and society, specifically by decreasing the cancer relapse rate, increasing the life expectancy and quality of life, and decreasing future rates of hospitalization, interventions, and costs.
The Role of Telomerase in Breast Cancer’s Response to Therapy
Authors: Judasz Eliza , Lisiak Natalia , Kopczyński Przemysław , Taube Magdalena , Rubiś Błażej
Published: October 2022

Currently, breast cancer appears to be the most widespread cancer in the world and the most common cause of cancer deaths. This specific type of cancer affects women in both developed and developing countries. Prevention and early diagnosis are very important factors for good prognosis. A characteristic feature of cancer cells is the ability of unlimited cell division, which makes them immortal. Telomeres, which are shortened with each cell division in normal cells, are rebuilt in cancer cells by the enzyme telomerase, which is expressed in more than 85% of cancers (up to 100% of adenocarcinomas, including breast cancer). Telomerase may have different functions that are related to telomeres or unrelated. It has been shown that high activity of the enzyme in cancer cells is associated with poor cell sensitivity to therapies. Therefore, telomerase has become a potential target for cancer therapies. The low efficacy of therapies has resulted in the search for new combined and more effective therapeutic methods, including the involvement of telomerase inhibitors and telomerase-targeted immunotherapy.
Comprehensive and Quantitative Analysis of the Changes in Proteomic and Phosphoproteomic Profiles during Stimulation and Repression of Steroidogenesis in MA-10 Leydig Cells
Authors: Demmouche Zoheir B. , Tremblay Jacques J.
Published: October 2022

Leydig cells produce testosterone, a hormone essential for male sex differentiation and spermatogenesis. The pituitary hormone, LH, stimulates testosterone production in Leydig cells by increasing the intracellular cAMP levels, which leads to the activation of various kinases and transcription factors, ultimately stimulating the expression of the genes involved in steroidogenesis. The second messenger, cAMP, is subsequently degraded to AMP, and the increase in the intracellular AMP levels activates AMP-dependent protein kinase (AMPK). Activated AMPK potently represses steroidogenesis. Despite the key roles played by the various stimulatory and inhibitory kinases, the proteins phosphorylated by these kinases during steroidogenesis remain poorly characterized. In the present study, we have used a quantitative LC-MS/MS approach, using total and phosphopeptide-enriched proteins to identify the global changes that occur in the proteome and phosphoproteome of MA-10 Leydig cells during both the stimulatory phase (Fsk/cAMP treatment) and inhibitory phase (AICAR-mediated activation of AMPK) of steroidogenesis. The phosphorylation levels of several proteins, including some never before described in Leydig cells, were significantly altered during the stimulation and inhibition of steroidogenesis. Our data also provide new key insights into the finely tuned and dynamic processes that ensure adequate steroid hormone production.
Identification of Clinical Variants beyond the Exome in Inborn Errors of Metabolism
Authors: Soriano-Sexto Alejandro , Gallego Diana , Leal Fátima , Castejón-Fernández Natalia , Navarrete Rosa , Alcaide Patricia , Couce María L. , Martín-Hernández Elena , Quijada-Fraile Pilar , Peña-Quintana Luis , Yahyaoui Raquel , Correcher Patricia , Ugarte Magdalena , Rodríguez-Pombo Pilar , Pérez Belén
Published: October 2022

Inborn errors of metabolism (IEM) constitute a huge group of rare diseases affecting 1 in every 1000 newborns. Next-generation sequencing has transformed the diagnosis of IEM, leading to its proposed use as a second-tier technology for confirming cases detected by clinical/biochemical studies or newborn screening. The diagnosis rate is, however, still not 100%. This paper reports the use of a personalized multi-omics (metabolomic, genomic and transcriptomic) pipeline plus functional genomics to aid in the genetic diagnosis of six unsolved cases, with a clinical and/or biochemical diagnosis of galactosemia, mucopolysaccharidosis type I (MPS I), maple syrup urine disease (MSUD), hyperphenylalaninemia (HPA), citrullinemia, or urea cycle deficiency. Eight novel variants in six genes were identified: six (four of them deep intronic) located in GALE, IDUA, PTS, ASS1 and OTC, all affecting the splicing process, and two located in the promoters of IDUA and PTS, thus affecting these genes’ expression. All the new variants were subjected to functional analysis to verify their pathogenic effects. This work underscores how the combination of different omics technologies and functional analysis can solve elusive cases in clinical practice.
Development and Applications of CRISPR/Cas9-Based Genome Editing in Lactobacillus
Authors: Mu Yulin , Zhang Chengxiao , Li Taihua , Jin Feng-Jie , Sung Yun-Ju , Oh Hee-Mock , Lee Hyung-Gwan , Jin Long
Published: October 2022

Lactobacillus, a genus of lactic acid bacteria, plays a crucial function in food production preservation, and probiotics. It is particularly important to develop new Lactobacillus strains with superior performance by gene editing. Currently, the identification of its functional genes and the mining of excellent functional genes mainly rely on the traditional gene homologous recombination technology. CRISPR/Cas9-based genome editing is a rapidly developing technology in recent years. It has been widely applied in mammalian cells, plants, yeast, and other eukaryotes, but less in prokaryotes, especially Lactobacillus. Compared with the traditional strain improvement methods, CRISPR/Cas9-based genome editing can greatly improve the accuracy of Lactobacillus target sites and achieve traceless genome modification. The strains obtained by this technology may even be more efficient than the traditional random mutation methods. This review examines the application and current issues of CRISPR/Cas9-based genome editing in Lactobacillus, as well as the development trend of CRISPR/Cas9-based genome editing in Lactobacillus. In addition, the fundamental mechanisms of CRISPR/Cas9-based genome editing are also presented and summarized.
Neurodegenerative Diseases: From Molecular Basis to Therapy
Authors: Agnello Luisa , Ciaccio Marcello
Published: October 2022

Neurodegenerative diseases (NDs) are a heterogeneous group of complex diseases characterized by neuronal loss and progressive degeneration of different areas of the nervous system [...]