bluuebunny/crossref_metadata_2025 huggingface dataset:
Emotion Analysis of Personalized Color of Beauty Package Based on NCD Image Positioning
Authors: Wang Feng , Xu Jifeng , Zhang Hanning , Yin Jun
Published: August 2021

Purpose:Based on the Nippon Color & Design (NCD) system, this study aims to accurately discover the color preferences and image positioning of different female groups in China and, thus, to establish a color reference system, which is suitable for the packaging of beauty products.

Methods:We first selected middle-aged women in the rural area of northern China as the studied group. Then, we conducted an extensive questionnaire survey on their color preferences focusing on their psychological characteristics and perception rules of emotional needs for colors. After that, we extracted colors from samples and classified them using the NCD system research method. Finally, we conducted a systematic analysis and verification to determine the color emotional preference of this group.

Conclusion:We investigate the color preference of targeted customers to establish the image space of beauty product packaging. By applying SPSS for the factor analysis, we study the rural women in northern China, who are less concerned about fashion. We focus on the accurate positioning between the interaction of color, women, and environment throughout the color design process. We have conducted a series of comparative analyses of popular beauty products among the rural women. The results can accurately reflect the brand definition and positioning of color semantics and, thus, provide invaluable information for future beauty packaging designs and marketing promotion strategy.
Chronic, Multi-Site Recordings Supported by Two Low-Cost, Stationary Probe Designs Optimized to Capture Either Single Unit or Local Field Potential Activity in Behaving Rats
Authors: Francoeur Miranda J. , Tang Tianzhi , Fakhraei Leila , Wu Xuanyu , Hulyalkar Sidharth , Cramer Jessica , Buscher Nathalie , Ramanathan Dhakshin R.
Published: August 2021

Rodent models of cognitive behavior have greatly contributed to our understanding of human neuropsychiatric disorders. However, to elucidate the neurobiological underpinnings of such disorders or impairments, animal models are more useful when paired with methods for measuring brain function in awake, behaving animals. Standard tools used for systems-neuroscience level investigations are not optimized for large-scale and high-throughput behavioral battery testing due to various factors including cost, time, poor longevity, and selective targeting limited to measuring only a few brain regions at a time. Here we describe two different “user-friendly” methods for building extracellular electrophysiological probes that can be used to measure either single units or local field potentials in rats performing cognitive tasks. Both probe designs leverage several readily available, yet affordable, commercial products to facilitate ease of production and offer maximum flexibility in terms of brain-target locations that can be scalable (32–64 channels) based on experimental needs. Our approach allows neural activity to be recorded simultaneously with behavior and compared between micro (single unit) and more macro (local field potentials) levels of brain activity in order to gain a better understanding of how local brain regions and their connected networks support cognitive functions in rats. We believe our novel probe designs make collecting electrophysiology data easier and will begin to fill the gap in knowledge between basic and clinical research.
Adjusting Reported COVID-19 Deaths for the Prevailing Routine Death Surveillance in India
Authors: Shewade Hemant Deepak , Parameswaran Giridara Gopal , Mazumder Archisman , Gupta Mohak
Published: August 2021

In India, the “low mortality” narrative based on the reported COVID-19 deaths may be causing more harm than benefit. The extent to which COVID-19 deaths get reported depends on the coverage of routine death surveillance [death registration along with medical certification of cause of death (MCCD)] and the errors in MCCD. In India, the coverage of routine death surveillance is 18.1%. This is compounded by the fact that COVID-19 death reporting is focused among reported cases and the case detection ratio is low. To adjust for the coverage of routine death surveillance and errors in MCCD, we calculated a correction (multiplication) factor at national and state level to produce an estimated number of COVID-19 deaths. As on July 31, 2020, we calculated the infection fatality ratio (IFR) for India (0.58:100–1.16:100) using these estimated COVID-19 deaths; this is comparable with the IFR range in countries with near perfect routine death surveillance. We recommend the release of excess deaths data during COVID-19 (at least in states with high death registration) and post-mortem COVID-19 testing as a surveillance activity for a better understanding of under-reporting. In its absence, we should adjust reported COVID-19 deaths for the coverage of routine death surveillance and errors in MCCD. This way we will have a clear idea of the true burden of deaths and our public health response will never be inadequate. We recommend that “reported” or “estimated” is added before the COVID-19 death data and related indicators for better clarity and interpretation.
It’s a Man’s World? Gender Spillover Effects on Performance in a Male-Dominated Industry
Authors: Kräft Charlotte
Published: August 2021

Getting more women into male-dominated industries has become the nucleus of public debate in many industrialized countries. However, it is still not clear how growing female representation impacts the individual performance of workers in these sectors. The research setting of this study is the Norwegian oil industry as a typically male-dominated sector. Using a fixed-effects regression model, the present paper investigates two different constellations: 1) how growing female representation impacts the individual performance of workers at the same hierarchical level (within-ranks); 2) how growing female representation at the next highest rank impacts the performance of subordinated workers (downward-flowing). Consistent with prevailing theory, the within-ranks analysis reveals that the performance of men in relation to a higher share of female peers follows a cubic pattern. This shows that men’s performance is the highest in gender-balanced teams. For women, this relationship cannot be confirmed. In terms of downward-flowing effects, female supervisors in this particular industry are estimated to have a negative effect on the performance of both, men and women. This result on negative downward-flowing effects requires a deeper analysis on the corporate cultural background.
Mycobacterial Cell Wall Stimulant in the Treatment of Squamous Cell Carcinoma: A Case Series Regarding Treatment in Equine, Bovine and Caprine Patients
Authors: Halleran Jennifer , Yau Katie , Paegelow Jillian , Streeter Robert , Foster Derek
Published: August 2021

Squamous cell carcinoma (SCC) is a common dermatological neoplasia found in large animal species. Treatment options, such as surgery and cryotherapy may be difficult or not feasible. Alternative therapies, such as immunomodulating drugs, can potentially be used for companion large animals. The hypothesis of the following retrospective study is: following multiple intravenous and intralesional injections of a mycobacterial cell wall stimulant (MCW) regression of SCC in equine, bovine and caprine patients will be observed. In this observational-retrospective case series, patients included are 2 bovine, 2 caprine and 3 equine patients. The medical records at two different teaching veterinary hospitals were searched for cases with a positive histopathological diagnosis of squamous cell carcinoma that were subsequently treated with MCW, as either the sole therapy, or in conjunction with other therapies. Seven cases were included in this retrospective study. The median duration of therapy was 56.5 days, with 3 of the 7 patients being euthanized. Significant complications were seen in 3/7 patients. Repeated injections of a MCW may lead to reduction in lesion size of SCC in some cases, but long-term resolution is unlikely and the risk of significant complications is high; due to limited sample size and the variety in species, it is difficult to conclude if MCW is an effective therapy for SCC.
Special Issue on Novel Approaches for Structural Health Monitoring
Authors: Surace Cecilia
Published: August 2021

Crucial mechanical systems and civil structures or infrastructures, such as bridges, railways, buildings, wind turbines, aeroplanes and more are subjected during their lifetime to natural deterioration of their structural integrity [...]
Work Category Affects the Exposure to Allergens and Endotoxins in an Animal Facility Laboratory in Italy: A Personal Air Monitoring Study
Authors: Di Renzi Simona , Chiominto Alessandra , Marcelloni Anna Maria , Melis Paola , Riviello Maria Cristina , Wirz Annarita , Sisto Renata , Massari Stefania , Paba Emilia , D’Ovidio Maria Concetta
Published: August 2021

Scientists and technicians who work in contact with laboratory animals are exposed to complex biological mixtures from animals, bedding and feed. The main objective of this study was to characterize the exposures to endotoxins and animal allergens in a biomedical research institution located in Central Italy by means of air sampling in the breathing zone of the staff during daily work activities. Forty-two inhalable dust samples were collected for endotoxins and allergens analysis. Filter extracts were analyzed using a Kinetic-QCL LAL kit for endotoxins; ELISA assays were performed for Mus m 1, Rat n 1, Can f 1, Fel d 1 and Equ c 4 detection. Laboratory animal attendants (LAAs) showed endotoxin concentrations significantly higher (4.59 ng/m3) than researchers (0.57 ng/m3), researchers working only in an office (0.56 ng/m3) and technicians (0.37 ng/m3). Endotoxin concentrations exceeding the recommended occupational exposure limit proposed by the Dutch Expert Committee on Occupational Safety were found in the case of two subjects in the animal attendants category. With regards to rat and mouse allergens, a higher average dose was found for mouse than rat allergens. Also for these bio-contaminants, the LAAs are confirmed as the work category at higher risk of exposure (15.85 ng/m3), followed by technicians (10.67 ng/m3), researchers (2.73 ng/m3) and researchers in an office (0.08 ng/m3). Fel d 1 was also detected (average: 0.11 ng/m3) highlighting a passive transport between living and occupational settings. Our data could be useful to improve the control and preventive measures, ensuring lower levels of allergens and endotoxins in animal facilities.
Effective Photocatalytic Hydrogen Evolution Using Covalent Triazine Framework-Derived Carbon Nitride Nanofiber Containing Carbon Vacancies for Visible-Light-Driven
Authors: Zhang Liangjing , Wang Aiwu , Zhang Mei , Jin Zhengyuan , Yi Huan , Wang Meng
Published: August 2021

In this study, a novel fibrous carbon nitride (FCN) was prepared from laminated covalent triazine framework (CTF) via pyrolysis, using functionalized 2,5-thiophenedicarboxylic acid and melamine as the precursors. A carbon vacancy was produced by two-step calcination in argon and air atmospheres. These carbon vacancies further exposed the edges and diffusion channels of the FCN nanofibers, which accelerated photogenerated charge transfer and provided more active sites. The FCN was characterized using various techniques and used for H2 evolution under visible-light irradiation. The as-synthesized FCN exhibited excellent stability, and its photocatalytic activity for H2 evolution under visible-light irradiation was 66 times higher than that of bare C3N4 (BCN), attaining a maximum H2 evolution rate of 102.63 μmol in 6 h. The FCN remained stable following visible-light irradiation at the end of 10 cycles. The FCN benefited from the absorption of solar energy and a large number of active sites. These advantages facilitated the efficient separation of photoexcited electron-hole pairs to hinder charge recombination. This work generates new insights into the preparation of highly effective FCN photocatalysts that may be put to various applications, especially in the fields of energy and environment.
Additivity between Key Odorants in Pig House Air
Authors: Hansen Michael Jørgen , Adamsen Anders Peter S. , Wu Chuandong , Feilberg Anders
Published: August 2021

The verification of odor abatement technologies for livestock production based on chemical odorants requires a method for conversion into an odor value that reflects the significance of the individual odorants. The aim of the present study was to compare the SOAV method (Sum of Odor Activity Values) with the odor detection threshold measured by olfactometry and to investigate the assumption of additivity. Synthetic pig house air with odorants at realistic concentration levels was used in the study (hydrogen sulfide, methanethiol, trimethylamine, butanoic acid, and 4-methylphenol). An olfactometer with only PTFE in contact with sample air was used to estimate odor threshold values (OTVs) and the odor detection threshold for samples with two to five odorants. The results show a good correlation (R2 = 0.88) between SOAV estimated based on the OTVs for panelists in the present study and values found in the literature. For the majority of the samples, the ratio between the odor detection threshold and SOAV was not significantly different from one, which indicates that the OAV for individual odorants in a mixture can be considered additive. In conclusion, the assumption of additivity between odorants measured in pig house air seems reasonable, but the strength of the method is determined by the OTV data used.
Seabirds in Conditions of Local Chronic Oil Pollution, Davis Sea, Antarctica
Authors: Golubev Sergey
Published: August 2021

Oil spills are rare in Antarctica. They threaten flying birds and penguins. This is the first report on the interactions of seabirds with oil in the area of the Mirny Station (East Antarctica). The purpose of the study is to determine the total number of seabird species interacting with oil in and around the Mirny Station, to assess the extent of pollution and to identify the most important sites of interactions. Oil pollution is found on the ground, on the continental ice and, on the seawater surface, both directly in the Mirny and beyond. Five species of seabirds were in contact with oil. Oil pollution threats have been identified for breeding and molting Adélie Penguins (Pygoscelis adeliae) and vagrant Macaroni Penguins (Eudyptes chrysolophus). Less affected by oil pollution during the breeding season were tube-nosed bird species and skuas. The most important places of interaction of seabirds with oil are at Cape Mabus, on the islands of Zykov, Tokarev, and Stroiteley. Evidence of long-term oil pollution of the environment is indicative of the chronic nature of the impacts on the coastal ecosystem.
Intravesical Instillation of Azacitidine Suppresses Tumor Formation through TNF-R1 and TRAIL-R2 Signaling in Genotoxic Carcinogen-Induced Bladder Cancer
Authors: Wang Shao-Chuan , Chang Ya-Chuan , Wu Min-You , Yu Chia-Ying , Chen Sung-Lang , Sung Wen-Wei
Published: August 2021

Azacitidine, an inhibitor of DNA methylation, shows therapeutic effects against several malignancies by inducing apoptosis and inhibiting tumor cell proliferation. However, the anti-tumor effects of azacitidine on urinary bladder urothelial carcinoma (UBUC), especially following intravesical instillation (IVI), are not established. Here, UBUC cell lines were used to analyze the in vitro therapeutic effects of azacitidine. Potential signaling pathways were investigated by antibody arrays and Western blotting. The N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)-induced rat UBUC model was used for in vivo quantitative analysis of tumor burden. Azacitidine significantly inhibited DNMT expression in UBUC cell lines and reduced cell viability and clonogenic activity, as determined by MTT and colony formation assays, while also inducing significant cytotoxic effects in the form of increased sub-G1 and Annexin V-PI populations (all p < 0.05). Antibody arrays confirmed the in vitro suppression of TNF-R1 and the induction of TRAIL-R2 and their downstream signaling molecules. TNF-R1 suppression reduced claspin and survivin expression, while TRAIL-R2 activation induced cytochrome C and caspase 3 expression. Rats with BBN-induced bladder cancer had a significantly reduced tumor burden and Ki67 index following IVI of azacitidine (p < 0.01). Our study provides evidence for a reduction in BBN-induced bladder cancer by IVI of azacitidine through alterations in the TRAIL-R2 and TNF-R1 signaling pathways. These findings might provide new insights for further clinical trials.
Predictors of Invasive Adenocarcinomas among Pure Ground-Glass Nodules Less Than 2 cm in Diameter
Authors: Hsu Wen-Chi , Huang Pei-Ching , Pan Kuang-Tse , Chuang Wen-Yu , Wu Ching-Yang , Wong Ho-Fai , Yang Cheng-Ta , Wan Yung-Liang
Published: August 2021

Benign lesions, atypical adenomatous hyperplasia (AAH), and malignancies such as adenocarcinoma in situ (AIS), minimally invasive adenocarcinoma (MIA), and invasive adenocarcinoma (IA) may feature a pure ground-glass nodule (pGGN) on a thin-slide computed tomography (CT) image. According to the World Health Organization (WHO) classification for lung cancer, the prognosis of patients with IA is worse than those with AIS and MIA. It is relatively risky to perform a core needle biopsy of a pGGN less than 2 cm to obtain a reliable pathological diagnosis. The early and adequate management of patients with IA may provide a favorable prognosis. This study aimed to disclose suggestive signs of CT to accurately predict IA among the pGGNs. A total of 181 pGGNs of less than 2 cm, in 171 patients who had preoperative CT-guided localization for surgical excision of a lung nodule between December 2013 and August 2019, were enrolled. All had CT images of 0.625 mm slice thickness during CT-guided intervention to confirm that the nodules were purely ground glass. The clinical data, CT images, and pathological reports of those 171 patients were reviewed. The CT findings of pGGNs including the location, the maximal diameter in the long axis (size-L), the maximal short axis diameter perpendicular to the size-L (size-S), and the mean value of long and short axis diameters (size-M), internal content, shape, interface, margin, lobulation, spiculation, air cavity, vessel relationship, and pleural retraction were recorded and analyzed. The final pathological diagnoses of the 181 pGGNs comprised 29 benign nodules, 14 AAHs, 25 AISs, 55 MIAs, and 58 IAs. Statistical analysis showed that there were significant differences among the aforementioned five groups with respect to size-L, size-S, and size-M (p = 0.029, 0.043, 0.025, respectively). In the univariate analysis, there were significant differences between the invasive adenocarcinomas and the non-invasive adenocarcinomas with respect to the size-L, size-S, size-M, lobulation, and air cavity (p = 0.009, 0.016, 0.008, 0.031, 0.004, respectively) between the invasive adenocarcinomas and the non-invasive adenocarcinomas. The receiver operating characteristic (ROC) curve of size for discriminating invasive adenocarcinoma also revealed similar area under curve (AUC) values among size-L (0.620), size-S (0.614), and size-M (0.623). The cut-off value of 7 mm in size-M had a sensitivity of 50.0% and a specificity of 76.4% for detecting IAs. In the multivariate analysis, the presence of air cavity was a significant predictor of IA (p = 0.042). In conclusion, the possibility of IA is higher in a pGGN when it is associated with a larger size, lobulation, and air cavity. The air cavity is the significant predictor of IA.
The Safety and Efficacy of Tranexamic Acid in Oncology Patients Undergoing Endoprosthetic Reconstruction and a ROTEM-Based Evaluation of Their Hemostatic Profile: A Pilot Study
Authors: Tsantes Andreas G. , Trikoupis Ioannis G. , Papadopoulos Dimitrios V. , Goumenos Stavros , Piovani Daniele , Nikolopoulos Georgios K. , Gialeraki Argyri , Bonovas Stefanos , Papagelopoulos Panayiotis J. , Kontogeorgakos Vasilios A. , Tsantes Argirios E.
Published: August 2021

Background: An endoprosthetic reconstruction in musculoskeletal oncology patients is associated with significant blood loss. The purpose of this study is to evaluate the safety and efficacy of tranexamic acid (TXA) for these patients and to assess any changes in their hemostatic profile using rotational thromboelastometry (ROTEM). Methods: A retrospective observational study was performed including 61 patients with primary or metastatic bone tumors who underwent surgery. Group A (n = 30) received both intravenous and local TXA whereas Group B (n = 31) was the control group. The primary outcomes were perioperative blood loss and blood unit transfusions and the secondary outcomes included the incidence of thromboembolic complications and a change in blood coagulability as reflected by ROTEM parameters. Results: The median difference in blood loss between the two groups was 548.5 mL, indicating a 29.2% reduction in the 72 h blood loss following TXA administration (p < 0.001). TXA also led to a reduced transfusion of 1 red blood cell (RBC) unit per patient (p < 0.001). The two groups had similar rates of thromboembolic complications (p = 0.99). The antifibrinolytic properties of TXA were confirmed by the significantly higher INTEM, FIBTEM and EXTEM LI60 (p < 0.001, p = 0.005 and p < 0.001, respectively) values in the TXA group. Conclusion: Tranexamic acid was associated with a significant reduction in perioperative blood loss and transfusion requirements without a complete shutdown of the fibrinolysis. Larger studies are warranted to assess the frequency of these outcomes in musculoskeletal oncology patients.
Risks and Function of Breast Cancer Susceptibility Alleles
Authors: Torabi Dalivandan Saeideh , Plummer Jasmine , Gayther Simon A.
Published: August 2021

Family history remains one of the strongest risk factors for breast cancer. It is well established that women with a first-degree relative affected by breast cancer are twice as likely to develop the disease themselves. Twins studies indicate that this is most likely due to shared genetics rather than shared epidemiological/lifestyle risk factors. Linkage and targeted sequencing studies have shown that rare high- and moderate-penetrance germline variants in genes involved in the DNA damage response (DDR) including BRCA1, BRCA2, PALB2, ATM, and TP53 are responsible for a proportion of breast cancer cases. However, breast cancer is a heterogeneous disease, and there is now strong evidence that different risk alleles can predispose to different subtypes of breast cancer. Here, we review the associations between the different genes and subtype-specificity of breast cancer based on the most comprehensive genetic studies published. Genome-wide association studies (GWAS) have also been used to identify an additional hereditary component of breast cancer, and have identified hundreds of common, low-penetrance susceptibility alleles. The combination of these low penetrance risk variants, summed as a polygenic risk score (PRS), can identify individuals across the spectrum of disease risk. However, there remains a substantial bottleneck between the discovery of GWAS-risk variants and their contribution to tumorigenesis mainly because the majority of these variants map to the non-protein coding genome. A range of functional genomic approaches are needed to identify the causal risk variants and target susceptibility genes and establish their underlying role in disease biology. We discuss how the application of these multidisciplinary approaches to understand genetic risk for breast cancer can be used to identify individuals in the population that may benefit from clinical interventions including screening for early detection and prevention, and treatment strategies to reduce breast cancer-related mortalities.
Increased HSP70 and TLR2 Gene Expression and Association of HSP70 rs6457452 Single Nucleotide Polymorphism with the Risk of Chronic Obstructive Pulmonary Disease in the Croatian Population
Authors: Hlapčić Iva , Grdić Rajković Marija , Čeri Andrea , Dabelić Sanja , Popović-Grle Sanja , Radić Antolic Margareta , Knežević Jelena , Försti Asta , Rumora Lada
Published: August 2021

Heat shock protein 70 (Hsp70) engages Toll-like receptors (TLR) 2 and 4 when found in the extracellular compartment and contributes to inflammation in chronic obstructive pulmonary disease (COPD). Since there is growing evidence for the genetic risk factors for COPD, the gene expression of HSP70, TLR2 and TLR4 was determined, as well as the association between HSP70, TLR2 and TLR4 single nucleotide polymorphisms, (SNPs) and COPD. The gene expression was assessed in peripheral blood cells of 137 COPD patients and 95 controls by a quantitative polymerase chain reaction (qPCR), while a total of nine SNPs were genotyped by TaqMan allelic discrimination real-time PCR. HSP70 and TLR2 gene expression was increased in COPD patients compared to the controls, regardless of the disease severity and smoking status of participants. The rs6457452 SNP of HSP70 was associated with COPD, indicating the protective role of the T allele (OR = 0.46, 95% CI = 0.24–0.89, p = 0.022). Furthermore, COPD C/T heterozygotes showed a decreased HSP70 mRNA level compared to COPD C/C homozygotes. In conclusion, HSP70 and TLR2 may have a role in the pathogenesis of COPD, and the HSP70 rs6457452 variant might influence the genetic susceptibility to COPD in the Croatian population.
Etiopathogenesis and Diagnostic Strategies in Autoimmune Hepatitis
Authors: Domerecka Weronika , Kowalska-Kępczyńska Anna , Michalak Agata , Homa-Mlak Iwona , Mlak Radosław , Cichoż-Lach Halina , Małecka-Massalska Teresa
Published: August 2021

Autoimmune hepatitis (AIH) is a chronic liver disease with the incidence of 10 to 17 per 100,000 people in Europe. It affects people of any age, but most often occurs in the 40–60 age group. The clinical picture is varied, from asymptomatic to severe acute hepatitis or liver failure. The disease onset is probably associated with the impaired function of T lymphocytes, the development of molecular mimicry, intestinal dysbiosis, or infiltration with low density neutrophils, which, alongside autoantibodies (i.e., ANA, ASMA), implicate the formation of neutrophil extracellular traps (NETs), as a component of the disease process, and mediate the inappropriate immune response. AIH is characterized with an increased activity of aminotransferases, elevated concentration of serum immunoglobulin G, the presence of circulating autoantibodies and liver inflammation. The result of the histological examination of the liver and the presence of autoantibodies, although not pathognomonic, still remain a distinguishing feature. The diagnosis of AIH determines lifelong treatment in most patients. The treatment is implemented to prevent the development of cirrhosis and end-stage liver failure. This work focuses mainly on the etiopathogenesis and diagnosis of AIH.
Species-Specific Nitrogen Resorption Efficiency in Quercus mongolica and Acer mono in Response to Elevated CO2 and Soil N Deficiency
Authors: Tobita Hiroyuki , Kitao Mitsutoshi , Uemura Akira , Utsugi Hajime
Published: August 2021

To test the effects of elevated CO2 and soil N deficiency on N resorption efficiency (NRE) from senescing leaves in two non-N2-fixing deciduous broadleaved tree species, Japanese oak (Quercus mongolica var. grosseserrata Blume) and Painted maple (Acer mono Maxim. var. glabrum (Lév. Et Van’t.) Hara), potted seedlings were grown in a natural daylight phytotron with either ambient or elevated CO2 conditions (36 Pa and 72 Pa CO2) and with two levels of N (52.5 and 5.25 mg N pot−1 week−1 for high N and low N, respectively). We examined the N content (Nmass) of mature and senescent leaves, as well as photosynthesis and the growth of plants, and calculated both the mass-based NRE (NREmass) and leaf area-based NRE (NREarea). In both species, the Nmass of mature leaves decreased with high CO2 and low N, whereas the leaf mass per area (LMA) increased under elevated CO2, regardless of N treatments. In Q. mongolica, both the maximum rate of carboxylation (Vcmax) and the maximum electron transport rate (Jmax) were reduced by elevated CO2 and low N, but Vcmax exhibited an interactive effect of N and CO2 treatments. However, in A. mono, both the Vcmax and Jmax decreased under elevated CO2, regardless of N treatments. The partitioning of N for the photosynthetic function within leaves was also significantly decreased by elevated CO2 in both species and increased under low N in A. mono. The Nmass of senesced leaves decreased under low N in both species and exhibited an increase (Q. mongolica) or no effect (A. mono) by elevated CO2. The NREarea of Q. mongolica was affected by CO2 and N treatments, with a decrease under elevated CO2 compared to ambient CO2 and under low N compared to high N. The NREarea of A. mono was also affected by CO2 and N treatments and decreased under elevated CO2; however, unlike in the case of Q. mongolica, it increased under low N. We speculate that these interspecific differences in the responses of leaf N allocation, indicated by the photosynthetic (Vcmax and Jmax) and morphological (LMA) responses to elevated CO2, may have affected the NRE during defoliation under high CO2 and soil N-deficient conditions.
Assessing Forest Level Response to the Death of a Dominant Tree within a Premontane Tropical Rainforest
Authors: Flores III Manuel R. , Aparecido Luiza Maria Teophilo , Miller Gretchen R. , Moore Georgianne W.
Published: August 2021

Small-scale treefall gaps are among the most important forms of forest disturbance in tropical forests. These gaps expose surrounding trees to more light, promoting rapid growth of understory plants. However, the effects of such small-scale disturbances on the distribution of plant water use across tree canopy levels are less known. To address this, we explored plant transpiration response to the death of a large emergent tree, Mortoniodendron anisophyllum Standl. & Steyerm (DBH > 220 cm; height ~40 m). Three suppressed, four mid-story, and two subdominant trees were selected within a 50 × 44 m premontane tropical forest plot at the Texas A&M Soltis Center for Research and Education located in Costa Rica. We compared water use rates of the selected trees before (2015) and after (2019) the tree gap using thermal dissipation sap flow sensors. Hemispherical photography indicated a 40% increase in gap fraction as a result of changes in canopy structure after the treefall gap. Micrometeorological differences (e.g., air temperature, relative humidity, and vapor pressure deficit (VPD)) could not explain the observed trends. Rather, light penetration, as measured by sensors within the canopy, increased significantly in 2019. One year after the tree fell, the water usage of trees across all canopy levels increased modestly (15%). Moreover, average water usage by understory trees increased by 36%, possibly as a result of the treefall gap, exceeding even that of overstory trees. These observations suggest the possible reallocation of water use between overstory and understory trees in response to the emergent tree death. With increasing global temperatures and shifting rainfall patterns increasing the likelihood of tree mortality in tropical forests, there is a greater need to enhance our understanding of treefall disturbances that have the potential to redistribute resources within forests.
Effects of Application Methods of Boron on Tomato Growth, Fruit Quality and Flavor
Authors: Xu Weinan , Wang Pengju , Yuan Luqiao , Chen Xin , Hu Xiaohui
Published: August 2021

The effect of application methods with different boron levels on the growth, fruit quality and flavor of tomato (Solanum lycopersicum L., cv. ‘Jinpeng No.1’) were investigated under greenhouse conditions. Seven treatments used included two application methods (leaf and root application) with four boron levels (0, 1.9, 3.8 and 5.7 mg∙L−1 H3BO3). Experimental outcomes revealed that both application methods significantly increased net photosynthetic rate and chlorophyll content, and stabilized leaf structure of tomato. Leaf spray of 1.9 mg∙L−1 H3BO3 was more effective at improving plant growth and photosynthetic indices in tomato compared to other treatments. Additionally, root application of 3.8 mg∙L−1 H3BO3 resulted in better comprehensive attributes of fruit quality and flavor than other treatments in terms of amounts of lycopene, β-carotene, soluble protein, the sugar/acid ratio and characteristic aromatic compounds in fruit. The appropriate application of boron can effectively improve the growth and development of tomato, and change the quality and flavor of fruit, two application methods with four boron levels had different effects on tomato.
Validation and Cultural Adaptation of Explanatory Model Interview Catalogue (EMIC) in Assessing Stigma among Recovered Patients with COVID-19 in Saudi Arabia
Authors: Al-Zamel Lamia A. , Al-Thunayan Shatha F. , Al-Rasheed Afnan A. , Alkathiri Munirah A. , Alamri Faisal , Alqahtani Faleh , Alali Amer S. , Almohammed Omar A. , Asiri Yousif A. , Bashatah Adel S. , AlRuthia Yazed
Published: August 2021

Stigma is a negative feeling affecting many patients with various health conditions, especially the contagious ones such as COVID-19. The Explanatory Model Interview Catalogue (EMIC) is one of the valid and reliable stigma-measuring tools; however, it has not been translated and validated in Arabic. Therefore, the aim of this study was to translate and validate the EMIC in Arabic among a sample of Arabic-speaking adults who recently recovered from COVID-19 in Saudi Arabia. The 12 items of the EMIC scale were forward- and backward-translated and reviewed by all authors to check the face and content validity prior to approving the final version of the Arabic 12-item EMIC. A total of 174 participants aged ≥18 years who contracted COVID-19 and recovered as of 29 July 2020 were interviewed. The Cronbach’s alpha of the Arabic version of the 12-item EMIC was 0.79, indicating an acceptable level of internal consistency. Using principal component analysis with varimax rotation, two factors explained more than 60% of the variance of the translated EMIC scale. The mean EMIC score was 5.91, implying a low level of stigma among participants. Married participants (β = 2.93; 95%CI 0.88 to 4.98, p = 0.005) and those with a family history of mental illness (β = 2.38; 95%CI 0.29 to 4.46, p = 0.025) were more likely to have higher EMIC scores in comparison to their counterparts who were unmarried and had no family history of mental illness. On the contrary, older adults were less likely to have high EMIC scores (β = −0.11; 95%CI −0.21 to −0.01, p = 0.03). Future studies with larger samples of patients with COVID-19 and various health conditions should be conducted to examine the validity and reliability of the Arabic version of the EMIC among different patient populations and to unveil the factors that may play a role in patients’ feelings of stigmatization in this part of the world.
Occurrence of Extended Spectrum Beta Lactamase (ESBL) Producers, Quinolone and Carbapenem Resistant Enterobacteriaceae Isolated from Environmental Samples along Msimbazi River Basin Ecosystem in Tanzania
Authors: Kimera Zuhura I. , Mgaya Fauster X. , Mshana Stephen E. , Karimuribo Esron D. , Matee Mecky I. N.
Published: August 2021

We conducted environmental surveillance of antimicrobial resistance (AMR) bacteria in the Msimbazi river basin in Tanzania to determine the occurrence of extended-spectrum β-lactamase (ESBL)-producing, carbapenem resistant Enterobacteriaceae (CRE) and quinolone resistant Escherichia coli and Klebsiella spp. A total of 213 Enterobacteriaceae isolates were recovered from 219 samples. Out of the recovered isolates, 45.5% (n = 97) were Klebsiella pneumoniae and 29.6% (n = 63) were Escherichia coli. K. pneumoniae isolates were more resistant in effluent (27.9%) compared to the E. coli (26.6%). The E. coli had a higher resistance in river water, sediment and crop soil than the K. pneumoniae (35 versus 25%), respectively. Higher resistance in K. pneumoniae was found in nalidixic acid (54.6%) and ciprofloxacin (33.3%) while the E. coli isolates were highly resistant to ciprofloxacin (39.7%) and trimethoprim/sulfamethoxazole (38%). Resistance increased from 28.3% in Kisarawe, where the river originates, to 59.9% in Jangwani (the middle section) and 66.7% in Upanga West, where the river enters the Indian Ocean. Out of 160 E. coli and K. pneumoniae isolates, 53.2% (n = 85) were resistant to more than three classes of the antibiotic tested, occurrence being higher among ESBL producers, quinolone resistant and carbapenem resistant strains. There is an urgent need to curb environmental contamination with antimicrobial agents in the Msimbazi Basin.
A Comprehensive Review on Types, Methods and Different Regions Related to Water–Energy–Food Nexus
Authors: Bian Zeyang , Liu Dan
Published: August 2021

Water, food, and energy are three of the most important resources for long-term survival and development. The term “nexus” is used to underline the need of controlling these primary components collectively rather than separately because they are interconnected and linked. With the purpose of better understanding nexus thinking and showcasing nexus analysis approaches and tools, this study explores the current state of the approach to the water–energy–food relationship, which has gotten a lot of attention in recent years. Water–energy, water–food, water–energy–food, water–energy, and climate are the four forms of nexus. This paper examines a variety of methodologies based on their principal objectives and provides a basic overview of a wide range of currently available methods and instruments for analyzing the water–energy–food (WEF) nexus. According to this study, the quantity of studies on the water–energy–food nexus has increased significantly, as the scientific community’s ability to analyze water, food, and energy interlinkages at a greater resolution. The integration and optimization of this multi-centric nexus is explored, with focus on four regions—Asia, Europe, America, and Africa—as a case study. The WEF nexus should be used in case studies to help illustrate its intricacies. Furthermore, this study builds a methodology and frameworks to find study linkages between water, energy, food, and other components, for a nexus analysis and discuss the major challenges and its solutions. This study also includes a scientometric analysis that looks at the countries and keyword mapping. Furthermore, the study is being planned, with an emphasis on quantitative analysis of the water–energy–food nexus which is helpful for the water security at local and global scale. This study aids in the coordination of research efforts to solve the difficult issues in nexus research and create sustainable and adaptable water, energy, and food systems.
Relation of Physical Activity Level to Postural Balance in Obese and Overweight Spanish Adult Males: A Cross-Sectional Study
Authors: Delfa-de la Morena José Manuel , Castro Eliane A. , Rojo-Tirado Miguel Ángel , Bores-García Daniel
Published: August 2021

The aim of this study was to analyze the influence of physical activity level on postural control in obese and overweight Spanish adult males. Forty-three males aged between 25 and 60 years old were included. Anthropometric, body composition, and physical activity variables were assessed, and postural control was evaluated using the Sensory Organization Test. No correlation was found between the level of physical activity and postural control, assessed by the Sensory Organization Test within the whole sample. However, within the group with a higher total fat mass percentage, non-sedentary individuals presented improved scores on the somatosensory organization test when compared to sedentary individuals (96.9 ± 1.8 vs. 95.4 ± 1.2; p < 0.05) and poorer scores on the composite equilibrium score (73.4 ± 7.2 vs. 79.2 ± 6.9; p < 0.05). The altered integration of somatosensory inputs most likely affects the tuning, sequencing, and execution of balance strategies in sedentary men with a high total fat mass percentage.
A Health Risk Assessment of Lead and Other Metals in Pharmaceutical Herbal Products and Dietary Supplements Containing Ginkgo biloba in the Mexico City Metropolitan Area
Authors: Rojas Patricia , Ruiz-Sánchez Elizabeth , Ríos Camilo , Ruiz-Chow Ángel , Reséndiz-Albor Aldo A.
Published: August 2021

The use of the medicinal plant Ginkgo biloba has increased worldwide. However, G. biloba is capable of assimilating both essential and toxic metals, and the ingestion of contaminated products can cause damage to health. The aim of this study was to investigate the safety of manganese (Mn), copper (Cu), lead (Pb), arsenic (As), and cadmium (Cd) in 26 items containing Ginkgo biloba (pharmaceutical herbal products, dietary supplements, and traditional herbal remedies) purchased in the metropolitan area of Mexico City. Metal analysis was performed using a graphite furnace atomic absorption spectrometer. All of the products were contaminated with Pb, 54% of them with As, and 81% with Cd. The lowest values of Pb, As, and Cd were detected in pharmaceutical herbal products > dietary supplements > traditional herbal remedies. The daily intake dose (DID) of pharmaceutical herbal products was within the established limits for the five metals. Dietary supplements and traditional herbal remedies exceeded the DID limits for Pb. The hazard quotients estimation and non-carcinogenic cumulative hazard estimation index for Mn, As, and Cd indicated no human health risk. Our results suggest that products containing G. biloba for sale in Mexico are not a health risk.
Methodological Framework for Resilience Assessment of Electricity Infrastructure in Conditions of Slovak Republic
Authors: Dvorak Zdenek , Chovancikova Nikola , Bruk Jozef , Hromada Martin
Published: August 2021

The quality of the environment as well as public health is convincingly coupled with the functioning of a power subsector. The power subsector plays a pivotal role in the sense that it emerges as the key cross-sectional element for the society’s functioning (production, services, healthcare, education and others). A modern society consists of infrastructure systems that are primarily dependent on continuous electricity supplies. Each and every element of the electric power infrastructure is unique, and thus, its malfunction can disrupt the functioning of an important part of the electric power infrastructure. In conjunction with ensuring the functioning of electric power infrastructure, our attention must be drawn to the resilience issue. As far as the resilience of electric power infrastructure is concerned, it can resist weather-related events ensuring there are no disruptions in continuous electricity supplies. First, in the introductory part, the article presents the legal framework in the Slovak Republic. Second, it describes the current state of the electric power infrastructure of Slovakia. Third, it handles the state of the level of security risk assessment. Later on, in the literature review, besides turning to the issue of resilience assessment, the authors focused on the area of resilience of power engineering. Furthermore, the article scrutinizes resilience assessment in Slovakia, and it briefly examines approaches towards natural threats. In addition, the article demonstrates several approaches towards flood resilience. Having used different methods, the primary concern is to devise a framework for resilience assessment. Therefore, the included case study examines aspects of the proposed framework for resilience assessment. In conclusion, our aim was, in most respects, to outline an innovative methodological framework for increasing the resilience of electricity infrastructure.
Injection of Anabolic Steroids in Men Who Had Sex with Men in Madrid and Barcelona: Prevalence Correlates and Role as a Risk Factor for Transmitted Infections
Authors: Guerras Juan-Miguel , Hoyos Juan , de la Fuente Luis , Román Francisca , Ayerdi Oskar , García-Pérez Jorge-Néstor , García de Olalla Patricia , Belza María-José ,   the Methysos Project Group
Published: August 2021

This study describes the prevalence of anabolic-androgenic steroid (AAS) injection, their main correlates, and the prevalence of specific AAS injection risk behaviours among men who have sex with men (MSM), an area insufficiently addressed in scientific research. Participants were HIV-negative MSM attending four HIV/STI diagnosis services: two clinics and two community programmes in Madrid and Barcelona. Participants answered an online self-administered questionnaire. Crude and adjusted lifetime prevalence and prevalence ratios (PRs) were calculated by different factors and using Poisson regression models with robust variance. Of the 3510 participants, 6.1% (95% CI: 5.3–6.9) had injected AAS before and 3.5% (95% CI: 2.9–4.2) had done so in the last 12 months. In the multivariate analysis, AAS injection was independently associated with being over 40 years old (aPR = 3.6; 95% CI: 2.0–6.5) and being born in Latin America (aPR = 2.5; 95% CI:1.9–3.4), and was less strongly associated (aPRs of around two) with having been recruited into STI clinics, having ever been paid for sex before, injected drugs, used drugs for sex, having been diagnosed with an STI before, and having been diagnosed with HIV at the recruitment consultation. Only three participants, 1.4%, of those who had injected AAS before had shared AAS or equipment for preparation or injecting before. Conclusions: In contrast to drugs, AAS injecting behaviours do not play a relevant, direct role in the transmission of blood-borne infections among MSM. However, AAS injectors have a higher prevalence of sexual risk behaviours. These findings should be confirmed using new studies that employ other sampling procedures.
Influence of Oral Health Care Systems on Future Career Environment of Dental Students in Europe
Authors: Wolf Thomas Gerhard , Otterbach Ernst-Jürgen , Zeyer Oliver , Wagner Ralf Friedrich , Crnić Tin , Ilhan Duygu , Campus Guglielmo
Published: August 2021

Oral healthcare is organized subsidiarily and independently by nation states in Europe and also within the EU and consequently, major differences between the nation states and the various oral healthcare systems in Europe are present. The socialization in the respective catchment area can have an impact on the job choice and the perception of employment opportunities of different professional groups. Therefore, the purpose of this survey was to elucidate the influence of different oral healthcare systems on students living or studying in the respective catchment area. A questionnaire (in English, French, German, Italian, and Spanish) with 18 different components was administered. Data on gender, age, country of origin, university, semester, nationality, expected time of graduation, and forecast for future professional practices were gathered. In addition, 3851 students participated (2863 f/988 m). The sample distribution was uneven with predominantly Bismarckian and Southern European System participants. The National oral health care system was statistically significantly linked (p < 0.01) to the ownership period of a dental practice. Students in Bismarckian and Nordic systems tended to find their own practice earlier than in the Beverdigian system or Southern European and Transitional—East European systems. An association between the oral health care system and vocational training was inhomogeneous, but also significantly different (p < 0.01). The majority (47.51%, n = 1555) would like to work in their own practice, 18.95% (n = 621) want to establish a practice with two or more owners. It was striking that no student would like to work in the investor practice/practice chain of both Nordic, Beveridgian and Transitional—East European countries systems (p < 0.01). The oral health care system in which a dental student grows up/resides/studies influences the career choice/perception of future professional practice.