bluuebunny/crossref_metadata_2025 huggingface dataset:
Guaranteeing consistently high quality Australian pork: are we any closer?
Authors: Channon H. A. , D'Souza D. N. , Dunshea F. R.
Published: November 2017

Considerable investment has been made by the Australian pork industry over several decades, to address key factors that affect pork quality, so as to improve consumer acceptability of pork and pork products. These outcomes have been utilised to inform on-farm quality assurance programs, develop effective solutions to negate boar taint issues associated with the production of entire males, drive continuous improvement in animal management and encourage new technologies to be implemented in both the production and processing sectors of the Australian pork supply chain. Australian Pork Limited’s Strategic Plan 2015–20 is focused on maintaining profitable and sustainable growth in existing markets and developing strong foundations to support new opportunities, both in Australia and internationally. Guaranteeing that pork available for purchase is always consistently high in eating quality will support ongoing consumer demand for pork through increased consumption frequency. However, achieving this on an everyday basis presents industry with significant challenges due to the many complex interactions among the production, processing and post-slaughter factors experienced by pigs, carcasses and pork that can influence final product quality, either singularly or in combination. The present paper describes recent quantitative studies to determine the size and effect of pathway parameters on eating quality attributes of fresh pork and knowledge gaps identified. Outcomes of consumer sensory studies to inform the development of a non-prescriptive cuts-based eating quality system for pork and commercially validate particular pathway interventions are detailed. Through the implementation of validated pathway interventions to optimise pork eating quality, the overall industry objective is to reduce eating quality fail rates of different pork cuts to less than 10%.
Novel marine polysaccharides and maternal nutrition to stimulate gut health and performance in post-weaned pigs
Authors: O'Doherty J. V. , Bouwhuis M. A. , Sweeney T.
Published: November 2017

Post-weaning complications in piglets are characterised by a reduction in feed intake and growth, atrophy of small-intestine architecture, upregulation of intestinal inflammatory cytokines, alterations in gastrointestinal microflora, diarrhoea and heightened susceptibility to infection. Traditional measures to reduce weaning-associated intestinal dysfunction have centred on dietary inclusion of antibiotic growth promoters in weaning pig diets, or high concentrations of dietary minerals in the form of zinc oxide. However, these strategies are under scrutiny because of their role in promoting multi-drug resistant bacteria and the accumulation of minerals in the environment. Up to recently, the main focus on finding alternatives to in-feed antibiotic growth promoters has been on dietary manipulations post-weaning, through the use of feed additives in the post-weaning diet. However, there are also other strategies that could enhance the growth and health of the newly weaned pig. One of these strategies is the use of maternal nutrition to improve growth and health in her offspring. The development of the immune system begins in utero and is further developed after the colonisation of the gastrointestinal tract with microbiota during birth and post-natal life. The early establishment of this relationship is fundamental to the development and long-term maintenance of gut homeostasis. There are significant efforts being made to identify natural alternatives to support the development of the piglet gastrointestinal tract, in particular during the weaning period. Chemodiversity in nature, including microorganisms, terrestrial plants, seaweeds and marine organisms, offers a valuable source of novel bioactives. This review will discuss the development of the intestinal tract in the pig during gestation, lactation and post-weaning periods and the factors that influence intestinal health post-weaning. It will also discuss how feeding marine bioactives in both the maternal diet and the piglet diet can be used to alleviate the negative effects associated with weaning.
The role of genomics in pig improvement
Authors: Garrick D. J.
Published: November 2017

Genomic prediction uses marker genotypes distributed throughout the genome to track the inheritance of chromosome fragments and quantify their contribution to the superiority or inferiority of breeding merit. It does this by using a so-called training population of historical animals with both genotype and phenotypic measures. Genotyping adds additional costs to an improvement program, so these costs must be offset elsewhere for there to be net benefit from adopting genomics in pig improvement. Genomic information is used implicitly or explicitly to predict the merit of young selection candidates more reliably than is the case when using only pedigree and phenotypic performance information. More accurate genomic prediction of index merit in young selection candidates results in faster genetic progress. Further, the technology allows good use to be made of phenotypic measures from non-traditional sources, including descendants of nucleus animals whose performance is measured in the commercial sector. This facilitates nucleus selection to include more reliable predictions for disease-resistance, and carcass and meat-quality traits, other traits with low heritability or those measured late in life, and to directly target selection for crossbred rather than purebred performance. Collectively, these features allow genomic prediction to provide a more balanced response to selection with respect to the entire portfolio of traits that influence income and costs in pig-production systems. Achieving the full cost–benefit potential from using genomics will not occur from simply genotyping nucleus animals and using this information in prediction, it requires innovation, ongoing phenotyping and genotyping, and re-examination of all the systems and processes involved in pig improvement.
Innovation in an expanding market: Australian pork is not a commodity
Authors: Bittner E. P. , Ashman H. , Hastie M. , van Barneveld R. J. , Hearn A. H. , Thomson N. , Dunshea F. R.
Published: November 2017

The growing Asian middle class, the proliferation of export markets and a more discerning domestic consumer base are creating new opportunities and challenges for the Australian pork industry. To fully capitalise on these opportunities and face these new challenges, the right questions need to be asked by the Australian pork industry. We need to know not only what our consumers want, but who our consumers are. The present paper aims to demonstrate that novel approaches to investigate consumer attitudes will be required, and it cannot be assumed that current productions systems, products and marketing strategies are optimal for the changing environment and the creation of new premium market opportunities. With new markets and new products come new consumers; identifying who those consumers are, the networks they operate within as food consumers, and what influences their purchasing decisions are the key to their adopting Australian pork as premium produce in a new global market.
Neurophysiological assessment of animal welfare
Authors: Tilbrook A. J. , Ralph C. R.
Published: November 2017

Livestock industries such as the pork industry are striving to continuously improve the welfare of animals. Inherent to the success of this is the ability to rigorously assess the welfare of animals in the field. While much progress has been made towards the development of methodology to assess the welfare of animals, there have been major challenges to establishing practical and definitive procedures to assess the welfare of animals. These include, but are not limited to, establishing a universally accepted definition of animal welfare and the choice of measures that are taken from the animal to assess its welfare. Measures of biological functioning and affective (emotional) state of the animal have been common, but there have been many limitations in terms of practical application. Some of the reasons for this include the choice of physiological measures, which are often restrictive in providing information about welfare, affective measures being restricted to specific behavioural measures and the biological-functioning and affective-states approaches being undertaken in isolation. Biological and affective functioning are integrated and controlled by the brain. Many of the regions of the brain involved in the regulation of biological and emotional functioning have been identified. Furthermore, there is considerable knowledge about the roles and interactions among the neurophysiological systems in these brain regions. We propose a strategy to use this knowledge to develop procedures to assess animal welfare. The initial phase is to identify the neural pathways that regulate the physiological and emotional processes that allow animals to adapt and cope. The next phase is to determine the activity of these pathways in conscious animals in the field. This requires the identification of biomarkers of specific neuronal activity that can be measured in the conscious animal in the field. Emerging technologies are offering promise in the identification of such biomarkers and some of these are already applicable to the pig. There is now the opportunity to apply this strategy within the pork industry to assess the welfare of pigs throughout the value chain.
Genetic improvement and dissemination for the global commercial swine industry
Authors: Culbertson M. S. , Herring W. O. , Holl J. W. , Casey D.
Published: November 2017

Commercial swine production has become an increasingly globalised industry, with global meat trade demanding that all regions compete on cost and differentiation of pork products. The utilisation of continually improving genetic populations can be one input that helps maintain, or increases, the competitiveness of an individual producer or regional industry. So as to deliver these improving genetic populations, genetic providers of today must focus on developing and implementing best science that delivers improvement on traits affecting commercial profitability. Providers must also efficiently multiply and disseminate the improved merit to the commercial hog production level. The swine-genetics industry has made considerable progress in driving a faster genetic gain over the past 30 years by systematically combining ever-changing computing power, accurate data capture and emerging genomics information. The combination of these technologies today has resulted in hundreds of thousands of animals being genotyped for tens of thousands of markers, and this information is being combined with extensive phenotypic data to deliver rates of genetic gain nearly double what we were able to achieve 20 years ago. As importantly, this scientific advancement can then be combined with the ability to continue to understand and evaluate emerging traits related to animal robustness, well-being and consumer demand, resulting in the most comprehensive definition of selection targets in the history of modern animal improvement. Finally, managing the dissemination of these genes through boar stud and multiplication systems helps ensure that the commercial level minimises lag and utilises the highest-merit genetics available.
What does the ‘closed herd’ really mean for Australian breeding companies and their customers?
Authors: Bunter K. L. , Hermesch S.
Published: November 2017

The perception that the genetic background of the Australian pig population is limiting for genetic improvement of commercial pigs in Australia is considered in the context of well established theory combined with practical evidence. The diversity of pig breeds used in modern commercial pig-breeding programs is diminished worldwide relative to all the pig breeds available. Australia is no different in this respect. The use of predominantly three main breeds (Large White, Landrace, Duroc) and synthetic lines, with contributions from other minor breeds to form the basis of a cross-breeding system for commercial pig production is well established internationally. The Australian concern of relatively small founder populations is potentially of relevance, from a theoretical perspective, for (1) the prevalence of defects or the presence of desirable alleles, and (2) the loss of genetic variation or increase in inbreeding depression resulting from increased inbreeding in closed nucleus lines, potentially reducing response to selection. However, rates of response achieved in Australian herds are generally commensurate with the performance recording and selection emphasis applied, and do not appear to be unduly restricted. Moreover, favourable alleles present in unrepresented breeds are frequently present in the three major breeds elsewhere, and therefore would be expected to be present within the Australian populations. Wider testing would provide confirmation of this. Comparison of estimates of effective population size of Australian populations with experimental selection lines overseas (e.g. INRA) or other intensely selected species (e.g. Holstein cattle) suggest adequate genetic diversity to achieve ongoing genetic improvement in the Australian pig industry. However, fitness traits should be included in breeding goals. What remains to be seen is whether novel phenotypes or genotypes are required to meet future challenges, which might be imposed by changes in the environment (e.g. climate change, disease) or market needs. Given probable overlap in genetic merit across Australian and foreign populations for unselected attributes, we suggest that sufficient genetic resources are already present in Australian herds to continue commercial progress within existing Australian populations that have adapted to Australian conditions.
Current and future antimicrobial resistance issues for the Australian pig industry
Authors: Abraham S. , O'Dea M. , Page S. W. , Trott D. J.
Published: November 2017

Antimicrobial use and antimicrobial resistance (AMR) in intensive pig production and its potential impacts to human and animal health are very much under the spotlight, both internationally, and within Australia. While the majority of AMR of medical importance is associated with the exclusive use of antimicrobials in humans, resistance in zoonotic foodborne pathogens such as Salmonella and Campylobacter, and livestock commensal bacteria such as Escherichia coli and Enterococcus spp., is under increased scrutiny. This is primarily due to the current reliance on many of the same drug classes as used in human medicine for treatment and control of bacterial diseases of livestock. Furthermore, the development of multidrug resistance in pathogens such as enterotoxigenic E. coli may drive off-label use of critically important drug classes such as 3rd-generation cephalosporins. This could lead to the emergence and amplification of resistance genes of potential public health significance in both pathogens and commensal bacteria. Livestock-associated and community-associated methicillin-resistant Staphylococcus aureus has also recently been detected in Australian pigs as a result of human-to-animal transmission and are a potential public health issue for in-contact piggery workers. Australia is in a unique position compared with many of its international trading partners due to its isolation, ban on importation of livestock and conservative approach to antimicrobial registration, including reservation of the fluoroquinolone class for use in humans and companion animals only. Cross-sectional AMR surveys of pathogens and commensals in healthy pigs have identified only low frequency of resistance to critically important drug classes. Nevertheless, resistance to critically important antimicrobials has emerged and careful antimicrobial stewardship is required to ensure that these low levels do not increase. In this report, we review AMR of significance to the Australian pig industry and identify potential prevention and control measures.
What are they thinking? Consumer attitudes to meat production in Australia
Authors: Bray H. J. , Buddle E. A. , Ankeny R. A.
Published: November 2017

Meat production has come under increasing scrutiny from consumers and citizens who feel that certain practices are unethical and negatively affect farm-animal welfare. Animal welfare can be viewed as both a scientific and social concept, and purchasing products with animal welfare claims can be considered an act of ‘ethical consumption’. The present paper reviews research that examines consumer attitudes to animal welfare and highlights tensions between consumer and citizen attitudes and behaviours, and assumptions that are made within these studies. We present our own research into motivations to purchase free-range eggs as an example of research that attempts to unpack these assumptions, in particular, that such purchases are made out of concern for animal welfare. We present a further example of our own research that attempts to identify how attitudes to meat production are socially constructed. We conclude with recommended strategies to engage the broader community in discussions about animal production, so as to improve industry–community communication about farm-animal welfare in meat-production industries.
Consumers want pork with ‘adjectives'
Authors: D'Souza D. N. , Cleary D. , Hewitt R. J. E.
Published: November 2017

Pork is the most consumed meat globally, but its consumption varies widely across the major pork-consuming nations. Consumers consider a wide variety of intrinsic and extrinsic cues, and credence attributes, when making purchasing and consumption decisions for food products. Brand recognition has been an important extrinsic cue for consumers, especially in the case of pork-product quality. However, the branding of fresh pork products in Australia has not been very prominent, due to the dominance of retailer ‘home-brand’ labels. However, increasingly these retailer labels are using information and branding relating to adjectives (credence attributes), for example, animal welfare, production systems, environment. The role of these credence attributes in Australia are now very much regarded by consumers as surrogate indicators of pork quality. The present paper will look at consumer preferences and attitudes to pork and the role credence attributes play when consumers purchase pork. In addition, the paper looks at the role of retailers in delivering pork with adjectives.
Plant circadian networks and responses to the environment
Authors: Muchapirei Chenjerai I. , Valentine Shannon-Leigh , Roden Laura C.
Published: November 2017

There are regular, and therefore predictable, environmental changes on Earth due to the rotation of the planet on its axis and its orbit around the sun. Thus organisms have adapted their metabolism, physiology and behaviour to minimise stresses caused by unfavourable conditions and maximise efficiency of growth. Additionally, most organisms are able to anticipate these changes and accordingly maximise metabolic efficiency and growth, because they have a complex biological time-keeping system commonly referred to as the circadian clock. Multiple pathways in plants are organised in a temporal manner through circadian clock-regulation of gene transcription and post-translational modifications. What is becoming more apparent is the bidirectional nature of interactions between the clock and stress response pathways. Until recently, the focus of many studies had been on the unidirectional, hierarchical control of biological processes by the circadian clock, and impacts on the clock in response to environmental stress had been largely ignored. Studies of interactions of the circadian clock with the environment have primarily been to understand mechanisms of entrainment. We review the evidence and implications of the reciprocal interactions between the clock and the environment.
The involvement of programmed cell death in inflated leaf petiole morphogenesis in Trapa pseudoincisa
Authors: Ni Xi-Lu , Tan Ling-ling , Zhou Ya-fu , Liu Wen-zhe , Li Chang-xiao
Published: November 2017

Trapa plants (Trapaceae) have an inflated leaf petiole called a spongy airbag. The aims of this study were to assess the involvement of programmed cell death (PCD) in the process of inflated leaf petiole morphogenesis. In this paper, light and transmission electron microscopy (TEM) were used to investigate cytological events and the development of inflated leaf petiole. During this process, the inflated leaf petiole of Trapa pseudoincisa L. undergoes a developmental process, changing from solid to hollow phase. Debris from the degraded cells was seldom observed in the transverse sections of leaf petioles, but some degraded cells with an abnormal morphology were observed in longitudinal sections. Cytoplasmic changes, such as disrupted vacuoles, degraded plastids, and the emergence of secondary vacuoles were observed during leaf petiole morphogenesis. In addition, gel electrophoresis and TUNEL assays were used to evaluate DNA cleavage during petiole morphogenesis. DNA internucleosomal cleavage and TUNEL-positive nuclei indicate that the typical PCD features of DNA cleavage occurred early in the process. These results revealed that PCD plays a critical role in inflated leaf petiole morphogenesis. Additionally, a trans-disciplinary systems approach is required that recognises the necessity for integration of cytological and molecular characteristics for identification of aerenchyma type.
Isolation and characterisation of microsatellites for the endangered Slater’s skink, Liopholis slateri (Squamata : Scincidae), via next-generation sequencing
Authors: Gardner Michael G. , Ansari Mina H. , Treilibs Claire E. , Johnston Angharad , Pavey Chris R. , Bull C. Michael
Published: November 2017

We characterised 14 new polymorphic microsatellite loci for the endangered lizard Liopholis slateri. Initially, 454 shotgun sequencing was used to identify 46 loci, which were trialled for amplification. Subsequently, 14 of these loci were screened for variation in 21 individuals from scat-derived DNA samples collected from Owen Springs Reserve in central Australia. All 14 loci were polymorphic, with observed heterozygosity ranging from 0.19 to 0.86 and the number of alleles per locus ranging from 2 to 10. These loci will be useful in understanding the genetic variation and connectivity within and among extant L. slateri populations.
Think tanks, Tories and the austerity discourse coalition
Authors: Pautz Hartwig
Published: November 2017

Political parties change their policy positions for a variety of reasons. Among these can be changing interpretations of economic or other kinds of knowledge. The article investigates whether think tanks, as producers, synthesisers and disseminators of knowledge, may have contributed to changes in the British Conservative Party’s positions on public spending, debt and deficit following the Global Financial Crisis and the onset of the Great Recession between 2008 and 2009. The article does not seek to attribute causality to think tanks’ activities and the Conservatives’ changing positions on public spending. However, it proposes that think tanks were relevant actors in the British ‘austerity discourse coalition’ and were, more specifically, relevant for the Conservatives’ adoption of a strict austerity agenda. The article also emphasises that think tanks should be considered as relevant actors in any analysis of the link between the cognitive dimension of public policy and political action.
Electrophysiological Measures of Aging Pharynx Function in C. elegans Reveal Enhanced Organ Functionality in Older, Long-lived Mutants
Authors: Russell Joshua Coulter , Burnaevskiy Nikolay , Ma Bridget , Mailig Miguel Arenas , Faust Franklin , Crane Matt , Kaeberlein Matt , Mendenhall Alexander
Published: November 2017

The function of the pharynx, an organ in the model system Caenorhabditis elegans, has been correlated with life span and motility (another measure of health) since 1980. In this study, in order to further understand the relationship between organ function and life span, we measured the age-related decline of the pharynx using an electrophysiological approach. We measured and analyzed electropharyngeograms (EPG) of wild type animals, short-lived hsf-1 mutants, and long-lived animals with genetically decreased insulin signaling or increased heat shock pathway signaling; we recorded a total of 2,478 EPGs from 1,374 individuals. As expected, the long-lived daf-2(e1370) and hsf-1OE(uthIs235) animals maintained pharynx function relatively closer to the youthful state during aging, whereas the hsf-1(sy441) and wild type animals’ pharynx function deviated significantly further from the youthful state at advanced age. Measures of the amount of variation in organ function can act as biomarkers of youthful physiology as well. Intriguingly, the long-lived animals had greater variation in the duration of pharynx contraction at older ages.
Development and psychometric properties of the Hookah Smoking Initiation for Women Questionnaire (HIWQ)
Authors: Shahbazi Sighaldeh S , Baheiraei A , Ebadi A , Khaki I , Kelishadi R , Majdzadeh R
Published: November 2017

The aims of this study were to design the Hookah Smoking Initiation for Women Questionnaire (HIWQ) and determine its psychometric properties. This was a sequential exploratory mixed-methods design consisting of qualitative and quantitative phases. This study was conducted from August 2012 to July 2013 in Tehran. In the qualitative phase, semi-structured interviews were held with 36 Iranian women for developing a preliminary item pool. Consequently, during the quantitative phase, the psychometric properties of the questionnaire were determined with the collaboration of 323 women living in various geographical locations in Tehran, Iran. Content validity of the questionnaire was examined by a panel of experts. The questionnaire’s construct validity was assessed using exploratory factor analysis and confirmatory factor analyses. The Cronbach’s alpha coefficient was calculated to check the questionnaire’s internal consistency reliability. Moreover, its stability was tested using the test–retest method. Exploratory factor analysis indicated that the HIQW could best be explained by a six-factor solution: ‘drawing the attention of other people’, the need to having fun and being relaxed, ‘hookah smoking in the family’, ‘availability of hookah’, ‘curiosity’ and ‘having a positive attitude toward hookah’. It also was found that the construct and content validity, and the reliability of the questionnaire were satisfactory (α = 0.83, ICC = 0.94). The HIQW was valid and reliable. Therefore, healthcare professionals can use it for evaluating the hookah smoking initiation in women. Future studies are required to refine this questionnaire and assess its applicability in different cultures and contexts.
Higher Moments of Arithmetic Functions in Short Intervals: A Geometric Perspective
Authors: Hast Daniel Rayor , Matei Vlad
Published: November 2017

We study the geometry associated to the distribution of certain arithmetic functions, including the von Mangoldt function and the Möbius function, in short intervals of polynomials over a finite field $\mathbb{F}_{q}$. Using the Grothendieck–Lefschetz trace formula, we reinterpret each moment of these distributions as a point-counting problem on a highly singular complete intersection variety. We compute part of the ℓ-adic cohomology of these varieties, corresponding to an asymptotic bound on each moment for fixed degree n in the limit as $q \to \infty $. The results of this paper can be viewed as a geometric explanation for asymptotic results that can be proved using analytic number theory over function fields.
Incidence trends, rates, and ethnic variations of primary CNS tumors in Texas from 1995 to 2013
Authors: Ambe Solomon N , Lyon Kristopher A , Nizamutdinov Damir , Fonkem Ekokobe
Published: November 2017

Background

Although rare, primary central nervous system (CNS) tumors are associated with significant morbidity and mortality. Texas is a representative sample of the United States population given its large population, ethnic disparities, geographic variations, and socio-economic differences. This study used Texas data to determine if variations in incidence trends and rates exist among different ethnicities in Texas.

Methods

Data from the Texas Cancer Registry from 1995 to 2013 were examined. Joinpoint Regression Program software was used to obtain the incidence trends and SEER*Stat software was used to produce average annual age-adjusted incidence rates for both nonmalignant and malignant tumors in Texas from 2009 to 2013.

Results

The incidence trend of malignant primary CNS tumors in whites was stable from 1995 to 2002, after which the annual percent change decreased by 0.99% through 2013 (95% CI, -1.4, -0.5; P = .04). Blacks and Asian/Pacific Islanders showed unchanged incidence trends from 1995 to 2013. Hispanics had an annual percent change of -0.83 (95% CI, -1.4, -0.2; P = .009) per year from 1995 through 2013. From 2009 to 2013, the incidence rates of nonmalignant and malignant primary CNS tumors were highest among blacks, followed by whites, Hispanics, Asians, and American Indians/Alaskan Natives.

Conclusions

Consistent with the 2016 Central Brain Tumor Registry of the United States report, the black population in Texas showed the highest total incidence of CNS tumors of any other race studied. Many factors have been proposed to account for the observed differences in incidence rate including geography, socioeconomic factors, and poverty factors, although the evidence for these external factors is lacking.
Risk Factors for Clinician-Diagnosed Lyme Arthritis, Facial Palsy, Carditis, and Meningitis in Patients From High-Incidence States
Authors: Kwit Natalie A , Nelson Christina A , Max Ryan , Mead Paul S
Published: November 2017

Background

Clinical features of Lyme disease (LD) range from localized skin lesions to serious disseminated disease. Information on risk factors for Lyme arthritis, facial palsy, carditis, and meningitis is limited but could facilitate disease recognition and elucidate pathophysiology.

Methods

Patients from high-incidence states treated for LD during 2005–2014 were identified in a nationwide insurance claims database using the International Classification of Diseases, Ninth Revision code for LD (088.81), antibiotic treatment history, and clinically compatible codiagnosis codes for LD manifestations.

Results

Among 88022 unique patients diagnosed with LD, 5122 (5.8%) patients with 5333 codiagnoses were identified: 2440 (2.8%) arthritis, 1853 (2.1%) facial palsy, 534 (0.6%) carditis, and 506 (0.6%) meningitis. Patients with disseminated LD had lower median age (35 vs 42 years) and higher male proportion (61% vs 50%) than nondisseminated LD. Greatest differential risks included arthritis in males aged 10–14 years (odds ratio [OR], 3.5; 95% confidence interval [CI], 3.0–4.2), facial palsy (OR, 2.1; 95% CI, 1.6–2.7) and carditis (OR, 2.4; 95% CI, 1.6–3.6) in males aged 20–24 years, and meningitis in females aged 10–14 years (OR, 3.4; 95% CI, 2.1–5.5) compared to the 55–59 year referent age group. Males aged 15–29 years had the highest risk for complete heart block, a potentially fatal condition.

Conclusions

The risk and manifestations of disseminated LD vary by age and sex. Provider education regarding at-risk populations and additional investigations into pathophysiology could enhance early case recognition and improve patient management.
Antibody and B Cell Subset Perturbations in Human Immunodeficiency Virus-Uninfected Patients With Cryptococcosis
Authors: Rohatgi Soma , Nakouzi Antonio , Carreño Leandro J , Slosar-Cheah Magdalena , Kuniholm Mark H , Wang Tao , Pappas Peter G , Pirofski Liise-anne
Published: November 2017

The importance of antibody immunity in protection against Cryptococcus neoformans remains unresolved. We measured serum C neoformans-specific and total antibody levels and peripheral blood B cell subsets of 12 previously healthy patients with cryptococcosis (cases) and 21 controls. Before and after adjustment for age, sex, and race, cryptococcal capsular polysaccharide immunoglobulin G was higher in cases than controls, whereas total B and memory B cell levels were lower. These associations parallel previous findings in patients with human immunodeficiency virus-associated cryptococcosis and suggest that B cell subset perturbations may also associate with disease in previously normal individuals with cryptococcosis.
Trans-sylvian Approach to Microvascular Decompression for Trigeminal Neuralgia in Syndromic Cranial Base Settling
Authors: Sarkar Mrityunjoy , Pillai Ashok
Published: November 2017

BACKGROUND AND IMPORTANCE

The lateral suboccipital approach for microvascular decompression (MVD) of the trigeminal nerve has become a standard-of-care over the past several decades. Syndromic cranial base settling, a rare but known cause for trigeminal neuralgia (TN), poses significant dilemmas in clinical management. In such cases, distorted anatomy may render surgery via the suboccipital approach difficult or even impossible.

CLINICAL PRESENTATION

A 34-yr-old male with osteogenesis imperfecta and severe basilar invagination suffered from TN that was refractory to medication and stereotactic radiosurgery. MVD of the fifth nerve root was performed via a pterional trans-sylvian approach after 3-dimensional trajectory modeling suggested the feasibility of this approach. He experienced complete resolution of TN and remains free of both pain and medications after 18 mo of follow-up.

CONCLUSION

This microsurgical trajectory, previously undescribed for TN, provides good visualization of the trigeminal root entry zone, thereby offering an excellent surgical option for severe TN in the setting of abnormal posterior fossa anatomy.
The Emergence of the Rehabilitative Strategy
Authors: Caracheo Armando , Bickenbach Jerome , Stucki Gerold
Published: November 2017

The aim of this essay was to show the impact that driving forces have had on the emergence of rehabilitation as a health strategy in the United States. Specifically, this essay (1) identifies the driving forces that have addressed the development of rehabilitation, (2) examines how the rehabilitative strategy has been driven by the driving forces through turning points and facilitators, and (3) identifies the barriers of each force against the development of rehabilitation. Critical and scoping review of the literature was conducted from the late 1910s until the end of the century. War, economy, the power of the state, and science were identified as the driving forces that led rehabilitation to become a health strategy complementing prevention, promotion, cure, and palliation. World War I and II played as a stimulus for rehabilitation, federal funding facilitated its development, acts, amendments, and governmental programs enabled its implementation, and the acknowledgment of physical medicine and rehabilitation as medical field contributed to its international recognition as health strategy. World Health Organization's International Classification of Functioning, Disability and Health is also identified as an emerging facilitator of rehabilitation. Based on the example of United States, this article closes with recommendations toward the implementation of rehabilitation as a health strategy in countries where so far this has not occurred.
Lymphoma Involving Skin and Subcutaneous Fat of the Breast
Authors: Kwon Miri , Ko Eun Young , Han Boo-Kyung , Ko Young Hye , Cho Eun Yoon , Cho Soo Youn , Kim Shin Young
Published: November 2017

Breast lymphoma is uncommon, and it usually involves breast parenchyma and ipsilateral axillary lymph nodes. We report rare cases of cutaneous T-cell lymphoma involving the skin and subcutaneous fat layers mimicking panniculitis of the breasts in a 33-year-old male patient who presented with a growing palpable mass and pain in both breasts and in 17-year-old female patient who presented with an indolent palpable breast mass for 1 year. Extranodal natural killer/T-cell nasal-type and subcutaneous panniculitis-like T-cell lymphomas were diagnosed after skin punch biopsies. Here we report the clinical manifestations, mammographic and ultrasound findings, and the difficulties in histological diagnosis of the cases.
Cleidocranial Dysplasia: A Review of Clinical, Radiological, Genetic Implications and a Guidelines Proposal
Authors: Farrow Emilie , Nicot Romain , Wiss Axel , Laborde Amélie , Ferri Joël
Published: November 2017

In this review, we aimed to depict the clinical, radiological, and genetic features of cleidocranial dysplasia (CCD) and to suggest management guidelines, based on our experience of 8 cases, with an emphasis given to dental complications.

The most common craniofacial features of CCD that stand out are a patency of the anterior fontanelle, an inverted pear-shaped calvaria, a hypertelorism, a general midface retrusion, and a mandible prognathism, associated with an excessive mobility of the shoulders, a short stature, and teeth abnormalities such as supernumerary teeth and failure of eruption, in particular. RUNX2 is the only gene in which mutation is known to cause CCD, but mutations are detected in only 65% of all patients with a clinical diagnosis of CCD. Panoramic radiography is a valuable adjunct in confirming the diagnosis of CCD.

Our experience allowed us to conclude that orthodontically aided eruption should always be attempted. However, to stabilize the occlusion and to improve facial esthetics, we recommend associated orthognathic surgery. When orthodontic treatment is partially efficient, prosthetic treatment options bring satisfactory results, in terms of occlusion. Nevertheless, when orthodontic treatment fails, we recommend to preserve as many native teeth as possible, and to combine orthognathic preprosthetic surgery and implant-supported prosthesis.

In any case, an individualized treatment protocol, depending on the needs and demand of the patient, the age at diagnosis and social and economic circumstances, should be put forward.
The Generation and Propagation of the Human Alpha Rhythm
Authors: Halgren Mila , Ulbert István , Bastuji Hélène , Fabó Dániel , Erőss Lorand , Rey Marc , Devinsky Orrin , Doyle Werner K. , Mak-McCully Rachel , Halgren Eric , Wittner Lucia , Chauvel Patrick , Heit Gary , Eskandar Emad , Mandell Arnold , Cash Sydney S.
Published: November 2017

The alpha rhythm is the longest studied brain oscillation and has been theorized to play a key role in cognition. Still, its physiology is poorly understood. In this study, we used micro and macro electrodes in surgical epilepsy patients to measure the intracortical and thalamic generators of the alpha rhythm during quiet wakefulness. We first found that alpha in posterior cortex propagates from higher-order anterosuperior areas towards the occipital pole, consistent with alpha effecting top-down processing. This cortical alpha leads pulvinar alpha, complicating prevailing theories of a thalamic pacemaker. Finally, alpha is dominated by currents and firing in supragranular cortical layers. Together, these results suggest that the alpharhythm likely reflects short-range supragranular feedback which propagates from higher to lower-order cortex and cortex to thalamus. These physiological insights suggest how alpha could mediate feedback throughout the thalamocortical system.
Length and time scales of cell-cell signaling circuits in agar
Authors: Doong Joy , Parkin James , Murray Richard M.
Published: November 2017

A community of genetically heterogeneous cells embedded in an unmixed medium allows for sophisticated operations by retaining spatial differentiation and coordinating division-of-labor. To establish the principles of engineering reliable cell-cell communication in a heterogeneous environment, we examined how circuit parameters and spatial placement affect the range of length and time scales over which simple communication circuits interact. We constructed several “sender” and “receiver” strains with quorum-sensing signaling circuits. The sender cell colony produces acyl homoserine lactones (AHL), which diffuse across the semisolid medium. The receiver cell colony detects these signal molecules and reports by fluorescence. We have found that a single colony of one sender variant is sufficient to induce receiver response at more than 1.5cm separation. Furthermore, AHL degradase expression in receiver colonies produces a signal threshold effect and reduces the response level in subsequent receiver colonies. Finally, our investigation on the spatial placement of colonies gave rise to the design of a multicellular long-range communication array consisting of two alternating colony types. Its signal response successfully propagated colony-by-colony along a six-colony array spanning 4.8cm at a transmission velocity of 12.8 hours per colony or 0.075cm per hour. In addition, we have developed a reaction-diffusion model that recreates the observed behaviors of the many performed experiments using data-informed parameter estimates of signal diffusion, gene expression, and nutrient consumption. These results demonstrate that a mixed community of colonies can enable new patterning programs, and the corresponding model will facilitate the rational design of complex communication networks.
Species comparison of liver proteomes reveals links to naked mole-rat longevity and human aging
Authors: Heinze Ivonne , Bens Martin , Calzia Enrico , Holtze Susanne , Dakhovnik Oleksandr , Sahm Arne , Kirkpatrick Joanna M. , Szafranski Karol , Romanov Natalie , Holzer Kerstin , Singer Stephan , Ermolaeva Maria , Platzer Matthias , Hildebrandt Thomas , Ori Alessandro
Published: November 2017

Mammals display wide range of variation in their lifespan. Investigating the molecular networks that distinguish long-from short-lived species has proven useful to identify determinants of longevity. Here, we compared the liver of long-lived naked mole-rats (NMRs) and the phylogenetically closely related, shorter-lived, guinea pigs using an integrated omic approach. We found that NMRs livers display a unique expression pattern of mitochondrial proteins that result in distinct metabolic features of their mitochondria. For instance, we observed a generally reduced respiration rate associated with lower protein levels of respiratory chain components, particularly complex I, and increased capacity to utilize fatty acids. Interestingly, we show that the same molecular networks are affected during aging in both NMR and humans, supporting a direct link to the extraordinary longevity of both species. Finally, we identified a novel longevity pathway and validated it experimentally in the nematodeC. elegans.
Cholesterol Synthesis Pathway Genes in Prostate Cancer are consistently downregulated when tissue confounding is minimized
Authors: Rye Morten Beck , Bertilsson Helena , Andersen Maria K. , Rise Kjersti , Bathen Tone F. , Drabløs Finn , Tessem May-Britt
Published: November 2017

The relationship between cholesterol and prostate cancer has been extensively studied for decades, where high levels of cellular cholesterol are generally associated with cancer progression and less favorable outcomes. However, the role of in vivo cellular cholesterol synthesis in this process is unclear, and data on the transcriptional activity of cholesterol synthesis pathway genes in tissue from prostate cancer patients are inconsistent. A common problem with cancer tissue data from patient cohorts is the presence of heterogeneous tissue which confounds molecular analysis of the samples. In this study we present a method to minimize systematic confounding from stroma tissue in seven patient cohorts consisting of 1713 prostate cancer and 230 normal tissue samples. When confounding was minimized, differential gene expression analysis over all cohorts showed robust and consistent downregulation of nearly all genes in the cholesterol synthesis pathway. Additional analysis also identified cholesterol synthesis as the most significantly altered metabolic pathway in prostate cancer. This surprising observation is important for our understanding of how prostate cancer cells regulate cholesterol levels in vivo. Moreover, we show that tissue heterogeneity explains the lack of consistency in previous expression analysis of cholesterol synthesis genes in prostate cancer.
Dihydrolipoamide dehydrogenase suppression induce human tau phosphorylation by increasing whole body glucose levels in aC.elegansmodel of Alzheimer’s Disease
Authors: Ahmad Waqar
Published: November 2017

The microtubule associated tau protein becomes hyperphosphorylated in Alzheimer’s disease (AD). While hyperphosphorylation promotes neurodegeneration, the cause and consequences of this abnormal modification are poorly understood. As impaired energy metabolism is an important hallmark of AD progression, we tested whether it could trigger phosphorylation of human tau protein in a transgenicC. elegansmodel of AD. We found that inhibition of a mitochondrial enzyme of energy metabolism, dihydrolipoamide dehydrogenase (DLD) resulted in elevated whole-body glucose levels as well as increased phosphorylation of tau. Hyperglycemia and tau phosphorylation were induced by either epigenetic suppression of thedld-1gene or by inhibition of the DLD enzyme by the inhibitor, 2-methoxyindole-2-carboxylic acid (MICA). Although the calcium ionophore A23187 could reduce tau phosphorylation induced by either chemical or genetic suppression of DLD, it was unable to reduce tau phosphorylation induced by hyperglycemia. While inhibition of thedld-1gene or treatment with MICA partially reversed the inhibition of acetylcholine neurotransmission by tau, neither treatment affected tau inhibited mobility. Conclusively, any abnormalities in energy metabolism were found to significantly affect the AD disease pathology.
Bayesian adaptive stimulus selection for dissociating models of psychophysical data
Authors: Cooke James R. H. , Selen Luc P. J. , van Beers Robert J. , Medendorp W. Pieter
Published: November 2017

Comparing models facilitates testing different hypotheses regarding the computational basis of perception and action. Effective model comparison requires stimuli for which models make different predictions. Typically, experiments use a predetermined set of stimuli or sample stimuli randomly. Both methods have limitations; a predetermined set may not contain stimuli that dissociate the models whereas random sampling may be inefficient. To overcome these limitations, we expanded the psi-algorithm (Kontsevich & Tyler, 1999) from estimating the parameters of a psychometric curve to distinguishing models. To test our algorithm, we applied it to two distinct problems. First, we investigated dissociating sensory noise models. We simulated ideal observers with different noise models performing a 2-afc task. Stimuli were selected randomly or using our algorithm. We found using our algorithm improved the accuracy of model comparison. We also validated the algorithm in subjects by inferring which noise model underlies speed perception. Our algorithm converged quickly to the model previously proposed (Stocker & Simoncelli, 2006), whereas if stimuli were selected randomly model probabilities separated slower and sometimes supported alternative models. Second, we applied our algorithm to a different problem; comparing models of target selection under body acceleration. Previous work found target choice preference is modulated by whole body acceleration (Rincon-Gonzalez et al., 2016). However, the effect is subtle making model comparison difficult. We show that selecting stimuli adaptively could have led to stronger conclusions in model comparison. We conclude that our technique is more efficient and more reliable than current methods of stimulus selection for dissociating models.

Data Availability

All data and code will be posted on our institutional repository system following acceptance. In the meantime feel free to contact the authors if you would like any of the code.