bluuebunny/crossref_metadata_2025 huggingface dataset:
Grid Quality Services from Smart Boilers: Experimental Verification on Realistic Scenarios for Micro-Grids with Demand-Side Management Oriented to Self-Consumption
Authors: Dimitrakakis Georgios S. , Georgakas Konstantinos G. , Topalis Evangelos S. , Vovos Panagis N.
Published: April 2024

The deeper penetration of renewables in the energy mix is an intense requirement in order to reduce global carbon dioxide emissions. In addition, new technologies are being developed, such as electric mobility and Distributed Generation (DG) in urban areas. However, the unpredictable fluctuations in energy generation from roof-installed PVs and the switching operation of their inverters greatly aggravate the already-present grid quality problems. In this paper, the Smart Boiler (SB) concept for grid quality improvement is presented. Furthermore, its experimental verification is implemented on a flexible testbed that accurately emulates several realistic scenarios for the low voltage distribution grid, under complex operating conditions. The proposed low-cost electronic kit, which contains a converter of fairly simple topology and requires connection to the internet, is used to upgrade conventional domestic boilers to smart devices. The SB automatically regulate the local reactive power flow, helping to stabilize the voltage level and suppress the grid current harmonic content, with both services provided in a matter of seconds. The higher the active power consumed and the denser the SB cluster, the wider the beneficial impact on the affected network area. While this service is provided, excess energy generated by PVs is temporarily stored as heat in the boiler tanks, given the users’ hot water consumption habits. The whole application, as a powerful demand-side management tool, proves beneficial for both the network operator and the end-user, especially when self-consumption is desirable in order to achieve a Nearly Zero Energy Building.
Agricultural Wastes and Their By-Products for the Energy Market
Authors: Zielińska Magdalena , Bułkowska Katarzyna
Published: April 2024

The conversion of lignocellulosic agricultural waste into biofuels and other economically valuable compounds can reduce dependence on fossil fuels, reduce harmful gas emissions, support the sustainability of natural resources, including water, and minimize the amount of waste in landfills, thus reducing environmental degradation. In this paper, the conversion of agricultural wastes into biomethane, biohydrogen, biodiesel, bioethanol, biobutanol, and bio-oil is reviewed, with special emphasis on primary and secondary agricultural residues as substrates. Some novel approaches are mentioned that offer opportunities to increase the efficiency of waste valorization, e.g., hybrid systems. In addition to physical, chemical, and biological pretreatment of waste, some combined methods to mitigate the negative effects of various recalcitrant compounds on waste processing (alkali-assisted thermal pretreatment, thermal hydrolysis pretreatment, and alkali pretreatment combined with bioaugmentation) are evaluated. In addition, the production of volatile fatty acids, polyhydroxyalkanoates, biochar, hydrochar, cellulosic nanomaterials, and selected platform chemicals from lignocellulosic waste is described. Finally, the potential uses of biofuels and other recovered products are discussed.
Elevational Earth-Sheltered Buildings with Horizontal Overhang Photovoltaic-Integrated Panels—New Energy-Plus Building Concept in the Territory of Serbia
Authors: Nešović Aleksandar , Kowalik Robert , Bojović Milan , Janaszek Agata , Adamczak Stanisław
Published: April 2024

The global scientific community is intensively promoting energy-plus buildings. Following the leading world trends, this paper presents a new energy-plus building concept—elevational earth-sheltered buildings with three different types of horizontal overhang photovoltaic-integrated panels: wooden support columns covered with clay tiles, steel pipes as support columns covered with sheet steel, and concrete support columns with concrete coverage. In this instance, the specific multi-numerical case study building model for the city of Kragujevac (located in central Serbia with favorable climatic conditions) was performed over 7 months (from 1 October to 30 April), taking into account the soil temperature, the effects of solar shading, the performance of the heating system—a ground source heat pump—and the characteristics of the artificial and automatic lighting control system. The simulation results show that the optimal depth of a horizontal overhang (energy-plus status) depends on the occupant’s habits, in addition to meteorological conditions. The presented methodology can be used for any other location, both in Europe and the world.
Design and Analysis of a Controllable Reactor Solid-State Circuit Breaker for Enhanced Fault Current Interruption in AC/DC Microgrids
Authors: Bakhshi Ali , Moghim Ali , Hojabri Mojgan
Published: April 2024

The occurrence of short-circuit faults in AC/DC microgrids gives rise to exceptionally high currents with rapid escalation, particularly in DC feeders where current zero-crossing is absent. This study introduces a comprehensive design procedure for a solid-state breaker tailored to address this challenge. A key innovation of the proposed solid-state circuit breaker lies in the incorporation of a current limiter reactor, which effectively constrains the current flow in both the load commutation switch and main breakers. Additionally, the inclusion of a resistive branch diminishes energy dissipation in the main breakers, safeguarding them against voltage stress. Consequently, the operational efficiency of the breaker is significantly enhanced, ensuring swift and efficient fault current interruption in vulnerable AC/DC microgrid scenarios. The efficacy of the proposed solid-state breaker was rigorously examined through analytical studies, and the results were validated using MATLAB/Simulink simulations. This breakthrough design represents a promising advancement in the realm of microgrid protection, offering a robust solution for mitigating the impact of short-circuit faults in AC/DC systems.
Simulation-Based Evaluation of the Impact of an Electrochromic Glazing on the Energy Use and Indoor Comfort of an Office Room
Authors: Teixeira Henriqueta , Moret Rodrigues A. , Aelenei Daniel , Gomes M. Glória
Published: April 2024

Electrochromic glazing alters its optical properties in the absence/presence of an electrical charge, varying from clear to dark to control daylighting and solar heat gains. This study aims to evaluate the impact of an electrochromic glazing, with indoor glare or temperature control, on the energy performance and thermal and visual comfort of an office room under three European climates, using a calibrated simulation model. The novelty of the paper lies in its combined performance assessment, using different standards and metrics. The results showed reduced climatization energy requirements with temperature control, but significantly increased artificial lighting energy use. Glare control achieved useful illuminance levels during 74–80% of working hours. Concerning temperature control, working hours within thermal comfort increased (21–43%) under a free-float regime. Moreover, the performance of this glazing was compared to that of a clear glazing with/without a reflective film and a thermochromic glazing for different solar orientations. The electrochromic glazing with glare control showed the highest energy savings (14–36%) for a western orientation, and the lowest negative impact on daylighting for a northern orientation. The best glare reduction was achieved with the reflective film. Considering the free-float regime, the electrochromic glazing, with temperature control, showed the highest increase in working hours within thermal comfort (6–9%) for a western orientation.
Development and Validation of a Novel Zero-Dimensional Heat Rejection Model for High-Efficiency Engines
Authors: Furia Francesca , Ravaglioli Vittorio , Cerofolini Alberto , Bussi Carlo
Published: April 2024

In recent years, the trend towards the performance maximization of modern internal combustion engines has led to the creation of accurate simulation models to optimize the engine design and operating conditions. Temperature management is crucial to achieve the performance goals of an internal combustion engine without affecting the component’s reliability. Formula 1 mandates that only a limited number of experimental tests can be performed, which leads to the necessity of simulators capable of substituting empirical tests. Furthermore, the requirement of adapting the vehicle setup before each race weekend to maximize the performance on each circuit layout necessitates short computational time. To address this, the development of a zero-dimensional model of the thermal flows within an engine is presented in this paper. This model allows to precisely compute the dynamic variations of all the heat flows inside the combustion engine, excluding only the radiative ones and the engine components’ temperatures. The new simulation approach has been developed and validated on a Formula 1 engine and shown to be precise and fast. The results demonstrate the value of the proposed model with an average engine fluid temperature error of less than 1 °C for a computational cost comparable with on-board applications.
Geochemical Characteristics of the Paleozoic Marine Source Rocks and Ultra-Deep Hydrocarbon Accumulation Mode of the Awati Sag
Authors: Song Zezhang , Zhang Ziyu , Ding Xiaoheng , Zhang Yuanyin , Bai Zhongkai , Liu Lihong , Gao Yongjin
Published: April 2024

The Lower Paleozoic of the Awati Sag and its periphery is a region with relatively low levels of exploration and stands as a frontier for ultra-deep hydrocarbon exploration. Based on outcrop and core samples, this study integrated organic geochemical analysis, total organic carbon (TOC) logging interpretation, and one-dimensional and two-dimensional hydrocarbon accumulation simulations, to clarify the primary source rock of the Lower Paleozoic and its characteristics, as well as its hydrocarbon accumulation mode. The findings indicate the following: (1) The Lower Paleozoic features two sets of industrial source rocks. The Yuertusi Formation, with its considerable thickness (approximately 200 m), widespread distribution, and elevated TOC (averaging approximately 5% from experimental data and logging interpretation), stands out as the Lower Paleozoic’s most pivotal source rock. (2) The Yuertusi and Saergan Formations are in a high-to-over-mature stage, with the Yuertusi initiating oil generation in the early Silurian and transitioning to gas by the late Permian. The Saergan began producing oil in the Carboniferous, followed by gas in the late Permian. (3) The potential ultra-deep gas reservoirs in the Awati Sag are mainly distributed in the structural traps closer to the deep faults in five potential target formations. Deep natural gas typically exhibits mixed-source signatures, with the mixing notably pronounced along the Shajingzi Fault Belt due to influential basin-controlling faults.
Coupling Mechanism of Multiple-Thermal-Fluid Multi-Cycle Stimulation in Ultra-Heavy-Oil Reservoirs
Authors: Ma Hongfei , Bo Bing , Xu Anzhu , Wang Shuqin , Wang Chenggang , Liu Minghui , Shan Fachao , Zhao Lun , Ma Gang
Published: April 2024

Multiple-thermal-fluid (MTF) stimulation technology has been successfully applied in heavy-oil reservoir development, resulting in the significant enhancement of oil production. However, the underlying mechanism of multi-component coupling remains unclear. This paper constructs a coupling model for MTF stimulation, investigates the coupling mechanism of different media in various zones during multiple-cycle stimulation operations, and compares the implementation effect with field results. The findings reveal that (1) based on media distribution, the area from near-wellbore to far well locations can be divided into four zones: high-temperature oil-viscosity-reduction zones, compound action zones, energy-replenishment zones, and unaffected zones. (2) In the high-temperature oil-viscosity-reduction zone, the latent heat of vaporization is released by steam, and ultra-heavy oil absorbs heat and reduces its viscosity, which plays a dominant role in the production of MTF. In the compound action zone, hot water, CO2, and N2 exhibit a synergistic effect which enhances overall performance. In the energy-replenishment zone, a small amount of N2 provides pressure maintenance and an additional energy supply. (3) As more cycles of stimulation are conducted, the compound action zone expands, while the energy-replenishment zone contracts. Simultaneously, there is a decrease in contribution rate from the high-temperature viscosity-reduction zone to oil production but an increase from both the compound action zone and energy-replenishment zone up to 30%. Based on the dynamic law of representative wells, this paper proposes a multi-media zonal coupling mechanism, providing a reference for subsequent research on MTF stimulation mechanisms and the adjustment of production measures.
Exploring Data Input Problems in Mixed Reality Environments: Proposal and Evaluation of Natural Interaction Techniques
Authors: Zhang Jingzhe , Chen Tiange , Gong Wenjie , Liu Jiayue , Chen Jiangjie
Published: April 2024

Data input within mixed reality environments poses significant interaction challenges, notably in immersive visual analytics applications. This study assesses five numerical input techniques: three benchmark methods (Touch-Slider, Keyboard, Pinch-Slider) and two innovative multimodal techniques (Bimanual Scaling, Gesture and Voice). An experimental design was employed to compare these techniques’ input efficiency, accuracy, and user experience across varying precision and distance conditions. The findings reveal that multimodal techniques surpass slider methods in input efficiency yet are comparable to keyboards; the voice method excels in reducing cognitive load but falls short in accuracy; and the scaling method marginally leads in user satisfaction but imposes a higher physical load. Furthermore, this study outlines these techniques’ pros and cons and offers design guidelines and future research directions.
Does the Artificial Enhancement and Release Activity Affect the Genetic Diversity of Marbled Rockfish Sebastiscus marmoratus in Zhoushan Waters?
Authors: Jiang Senping , Chen Xinyi , Li Pengfei , Wang Haoxue , Li Jiji , Xu Kaida , Ye Yingying
Published: April 2024

Artificial enhancement and release activity is an important method in the restoration of fishery resources. In order to understand the possible genetic effect of hatchery-released populations on wild populations during the artificial enhancement and release activities of Sebastiscus marmoratus in Zhoushan waters, we utilized mitochondrial DNA control region sequences to examine the genetic diversity in four S. marmoratus populations, including one farmed population, one released population and two wild populations. A total of 68 haplotypes from 123 individuals were detected, including 3 shared haplotypes. Haplotype diversity ranged from 0.944 to 0.980, with a mean of 0.966. The nucleotide diversity ranged from 0.020 to 0.025, with a mean of 0.022. Analysis of Molecular Variance (AMOVA) indicated that the primary genetic variation occurs within populations and the index of genetic differentiation between populations (FST) among the four populations showed no differentiation. The results indicate that the current artificial enhancement and release has not impacted the S. marmoratus population in Zhoushan waters. Continued long-term monitoring is essential to protect the high-quality germplasm resources of S. marmoratus.
Emotional Eating among College Students in Israel: A Study during Times of War
Authors: Houminer Klepar Nourit , Davidovitch Nadav , Dopelt Keren
Published: April 2024

Emotional eating, the act of consuming food to cope with negative emotions rather than responding to hunger cues, can lead to overeating in an attempt to regulate and alleviate these emotions. This study aimed to assess emotional eating among college students in Israel, specifically during times of war, which present unique and heightened stressors that accumulate on top. A total of 575 participants from the Ashkelon Academic College completed an online questionnaire examining background information, stress levels, and emotional eating symptoms. Our findings indicate that factors, such as being female, not having children, younger age, lower body satisfaction, higher BMI, and increased stress, are predictors of heightened emotional eating. These results highlight risk factors predisposing college students to engage in emotional eating. Developing targeted interventions, particularly campus-based programs to address emotional eating by promoting healthy coping strategies, a positive body image, and stress management skills is needed. In addition, raising awareness concerning emotional eating risks during challenging life transitions and distressing situations is necessary. The college leadership, led by the departments of Nutrition, Psychology, and Public Health, in collaboration with stakeholders in the Israeli Ministry of Health, must consider the mental effects of war on students and their involvement in emotional eating.
Pharmacological Significance of Boraginaceae with Special Insights into Shikonin and Its Potential in the Food Industry
Authors: Gautam Shweta , Lapčík Lubomír , Lapčíková Barbora
Published: April 2024

Shikonin is a naphthoquinone pigment present in the hairy roots of the plant species from the Boraginaceae family. The compound has been well investigated for its highly efficient medicinal, antioxidant, and antimicrobial properties. Various extraction methodologies have been employed to maximise yield while minimising waste production of shikonin and its derivatives. Despite substantial research on shikonin and Boraginaceae plants, a research gap persists in the food industry and extraction technologies. This review addresses crucial aspects of shikonin deserving of further exploration. It begins by elucidating the attributes of the Boraginaceae plants and their medicinal traits in folklore. It proceeds to focus on the roots of the plant and its medicinal properties, followed by extraction procedures explored in the last fifteen years, emphasising the novel technologies that have been chosen to improve the yield extract while minimising extraction times. Furthermore, this review briefly outlines studies employing cell culture techniques to enhance in vitro shikonin production. Lastly, attention is directed towards research in the food industry, particularly on shikonin-loaded biodegradable films and the antioxidant activity of shikonin. This review concludes by summarising the future potential in food science and prominent research gaps in this field.
Metabolic Syndrome According to Dietary and Health-Related Lifestyle in Male Cancer Survivors and Non-Cancer over 40 Years of Age
Authors: Meng Huan , Choi Yongchul , Yim Kitae
Published: April 2024

Researchers often report higher metabolic syndrome (MetS) pr\4;evalence among cancer survivors than among non-cancer individuals. This study aims to explore the impact of cancer presence, activity type, and dietary lifestyle on MetS in males over 40 years of age. Participants (n = 9846; 618 cancer survivors, 9228 non-cancer) were selected by extracting data from a Korean government database spanning the years 2016 to 2021. Physical activity patterns, dietary habits, and MetS factors were measured, and a multiple logistic regression analysis was statistically processed for an odds ratio (OR). MetS was present in 32.8% of cancer survivors and 28.6% of non-cancer individuals. Gastric cancer survivors exhibited a 16% lower OR for MetS versus non-cancer participants. The ORs were higher by 1.60-, 1.45-, and 1.26-fold for colorectal, urinary, and other cancers, respectively. Cancer survivors with high calorie, carbohydrate, and fat intakes exhibited ORs of 2.01 (95% CI 1.28−4.04), 2.33 (95% CI 1.28−4.54), and 1.39 (95% CI 1.05−2.37) compared to the recommended level. The high fiber-intake group reduced the MetS OR by 20%. In conclusion, The MetS prevalence was higher in survivors with colorectal cancer, urinary cancer, and other cancers, while it was lower in patients with gastric cancer. Survivors with low rates of eating three meals a day, high skipping breakfast, increased eating-out rate, and no nutritional learning opportunity displayed higher MetS prevalence. Additionally, cancer survivors who had more strength and leisure activities had a lower OR of MetS.
Chitosan Coating with Rosemary Extract Increases Shelf Life and Reduces Water Losses from Beef
Authors: de Lima Allison F. , Leite Ricardo H. de L. , Pereira Marília W. F. , Silva Maria R. L. , de Araújo Thiago L. A. C. , de Lima Júnior Dorgival M. , Gomes Marina de N. B. , Lima Patrícia de O.
Published: April 2024

This study aimed to evaluate the influence of films based on chitosan and rosemary extract on the physicochemical, microbiological, and oxidative characteristics of beef. Refrigerated steaks of Longissimus dorsi were distributed in a factorial arrangement (4 × 4) into four treatments consisting of four edible films (control; chitosan; chitosan + 4% rosemary extract; and chitosan + 8% rosemary extract) and four days of aging (0, 2, 4, and 8 days). Incorporating 4% or 8% rosemary extract into the chitosan film improved the characteristics of the films in terms of moisture absorption and elasticity. The edible coatings with chitosan and rosemary extract and the different days of aging increased the tenderness and decreased the lipid oxidation of beef. In addition, the chitosan films containing rosemary extract increased the water-holding capacity and decreased the cooking losses of beef. The films containing 4% and 8% rosemary extract decreased the development of mesophilic and psychrotrophic bacteria and Staphylococcus ssp. in beef. We recommend incorporating 4% rosemary extract into chitosan-based coatings to preserve the quality of refrigerated beef.
Effect of Chitosan and Hyperbranched Poly-L-Lysine Treatment on Quality of Cucumber (Cucumis sativus L.) during Storage
Authors: Sun Jianrui , Li Jinglan , Ren Ruirui , Yao Linlin , Tong Li , Yuan Jiangfeng , Wang Dahong
Published: April 2024

To enhance the storage time of cucumbers, this research investigated the impact of chitosan (CS) and hyperbranched poly-L-lysine (HBPL) on the quality and nutritional attributes of cucumbers when stored at a temperature of 25 °C. The results demonstrated that sensory evaluation scores for cucumbers treated with a CS–HBPL combination were significantly higher than the control (CK), CS, and HBPL groups. On the 18th day of storage, cucumbers in the CK group exhibited significant decay and softening; however, there was a decrease in hardness observed in the CS–HBPL group and no decay or noticeable sour taste was detected. Furthermore, compared to the CK group, treatment with CS–HBPL effectively delayed cucumber decay and weight loss rate while significantly inhibiting decreases in cucumber hardness and growth of surface microorganisms. Additionally, it substantially reduced losses of soluble protein content as well as vitamin C (Vc), reducing sugars, and total phenolic compounds within cucumbers, which were 4.7 mg/g, 4.7 mg/g, 0.94 mg/g, and 0.52 mg/kg, respectively. Moreover, compared to the CK group, combined treatment with CS–HBPL significantly inhibited malondialdehyde (MDA) accumulation and reducing relative electrolyte permeability within cucumbers, which were 1.45 μmol·g−1FW and 29.82%. Furthermore, it notably enhanced activities of superoxide dismutase (SOD) and catalase (CAT), while exerting a significant inhibitory effect on polyphenol oxidase (PPO). In summary, the combined CS–HBPL treatment successfully prolonged cucumber shelf life at room temperature, enabling new possibilities for extending cucumber shelf life.
Canola Seed Protein: Pretreatment, Extraction, Structure, Physicochemical and Functional Characteristics
Authors: Zhu Huipeng , Wang Lu , Li Xiaoyu , Shi John , Scanlon Martin , Xue Sophia , Nosworthy Matthew , Vafaei Nazanin
Published: April 2024

The rapid growth of the global population has led to an unprecedented demand for dietary protein. Canola seeds, being a widely utilized oil resource, generate substantial meal by-products following oil extraction. Fortunately, canola meals are rich in protein. In this present review, foremost attention is directed towards summarizing the characteristics of canola seed and canola seed protein. Afterwards, points of discussion related to pretreatment include an introduction to pulsed electric field treatment (PEF), microwave treatment (MC), and ultrasound treatment (UL). Then, the extraction method is illustrated, including alkaline extraction, isoelectric precipitation, acid precipitation, micellization (salt extraction), and dry fractionation and tribo-electrostatic separation. Finally, the structural complexity, physicochemical properties, and functional capabilities of rapeseed seeds, as well as the profound impact of various applications of rapeseed proteins, are elaborated. Through a narrative review of recent research findings, this paper aims to enhance a comprehensive understanding of the potential of canola seed protein as a valuable nutritional supplement, highlighting the pivotal role played by various extraction methods. Additionally, it sheds light on the broad spectrum of applications where canola protein demonstrates its versatility and indispensability as a resource.
Production of Plant-Based, Film-Type Scaffolds Using Alginate and Corn Starch for the Culture of Bovine Myoblasts
Authors: Lee Jun-Yeong , Kamel Jihad , Yadav Chandra-Jit , Yadav Usha , Afrin Sadia , Son Yu-Mi , Won So-Yeon , Han Sung-Soo , Park Kyung-Mee
Published: April 2024

Natural scaffolds have been the cornerstone of tissue engineering for decades, providing ideal environments for cell growth within extracellular matrices. Previous studies have favored animal-derived materials, including collagen, gelatin, and laminin, owing to their superior effects in promoting cell attachment, proliferation, and differentiation compared to non-animal scaffolds, and used immortalized cell lines. However, for cultured meat production, non-animal-derived scaffolds with edible cells are preferred. Our study represents the first research to describe plant-derived, film-type scaffolds to overcome limitations associated with previously reported thick, gel-type scaffolds completely devoid of animal-derived materials. This approach has been employed to address the difficulties of fostering bovine muscle cell survival, migration, and differentiation in three-dimensional co-cultures. Primary bovine myoblasts from Bos Taurus Coreanae were harvested and seeded on alginate (Algi) or corn-derived alginate (AlgiC) scaffolds. Scaffold functionalities, including biocompatibility and the promotion of cell proliferation and differentiation, were evaluated using cell viability assays, immunofluorescence staining, and reverse transcription-quantitative polymerase chain reaction. Our results reveal a statistically significant 71.7% decrease in production time using film-type scaffolds relative to that for gel-type scaffolds, which can be maintained for up to 7 days. Film-type scaffolds enhanced initial cell attachment owing to their flatness and thinness relative to gel-type scaffolds. Algi and AlgiC film-type scaffolds both demonstrated low cytotoxicity over seven days of cell culture. Our findings indicated that PAX7 expression increased 16.5-fold in alginate scaffolds and 22.8-fold in AlgiC from day 1 to day 3. Moreover, at the differentiation stage on day 7, MHC expression was elevated 41.8-fold (Algi) and 32.7-fold (AlgiC), providing initial confirmation of the differentiation potential of bovine muscle cells. These findings suggest that both Algi and AlgiC film scaffolds are advantageous for cultured meat production.
Quantitative Proteomics Reveals the Relationship between Protein Changes and Volatile Flavor Formation in Hunan Bacon during Low-Temperature Smoking
Authors: Zou Huiyu , Deng Chuangye , Li Junnian , Lou Aihua , Liu Yan , Luo Jie , Shen Qingwu , Quan Wei
Published: April 2024

This study aimed to investigate the changes in proteins and volatile flavor compounds that occur in bacon during low-temperature smoking (LTS) and identify potential correlations between these changes. To achieve this, a combination of gas chromatography-mass spectrometry and proteomics was employed. A total of 42 volatile flavor compounds were identified in the bacon samples, and, during LTS, 11 key volatile flavor compounds with variable importance were found at a projection value of >1, including 2′,4′-dihydroxyacetophenone, 4-methyl-2H-furan-5-one, Nonanal, etc. In total, 2017 proteins were quantified at different stages of LTS; correlation coefficients and KEGG analyses identified 27 down-regulated flavor-related proteins. Of these, seven were involved in the tricarboxylic acid (TCA) cycle, metabolic pathways, or amino acid metabolism, and they may be associated with the process of flavor formation. Furthermore, correlation coefficient analysis indicated that certain chemical parameters, such as the contents of free amino acids, carbonyl compounds, and TCA cycle components, were closely and positively correlated with the formation of key volatile flavor compounds. Combined with bioinformatic analysis, the results of this study provide insights into the proteins present in bacon at various stages of LTS. This study demonstrates the changes in proteins and the formation of volatile flavor compounds in bacon during LTS, along with their potential correlations, providing a theoretical basis for the development of green processing methods for Hunan bacon.
Cultivation of Bovine Mesenchymal Stem Cells on Plant-Based Scaffolds in a Macrofluidic Single-Use Bioreactor for Cultured Meat
Authors: Gome Gilad , Chak Benyamin , Tawil Shadi , Shpatz Dafna , Giron Jonathan , Brajzblat Ilan , Weizman Chen , Grishko Andrey , Schlesinger Sharon , Shoseyov Oded
Published: April 2024

Reducing production costs, known as scaling, is a significant obstacle in the advancement of cultivated meat. The cultivation process hinges on several key components, e.g., cells, media, scaffolds, and bioreactors. This study demonstrates an innovative approach, departing from traditional stainless steel or glass bioreactors, by integrating food-grade plant-based scaffolds and thermoplastic film bioreactors. While thermoplastic films are commonly used for constructing fluidic systems, conventional welding methods are cost-prohibitive and lack rapid prototyping capabilities, thus inflating research and development expenses. The developed laser welding technique facilitates contamination-free and leakproof sealing of polyethylene films, enabling the efficient fabrication of macrofluidic systems with various designs and dimensions. By incorporating food-grade plant-based scaffolds, such as rice seeded with bovine mesenchymal stem cells, into these bioreactors, this study demonstrates sterile cell proliferation on scaffolds within macrofluidic systems. This approach not only reduces bioreactor prototyping and construction costs but also addresses the need for scalable solutions in both research and industrial settings. Integrating single-use bioreactors with minimal shear forces and incorporating macro carriers such as puffed rice may further enhance biomass production in a scaled-out model. The use of food-grade plant-based scaffolds aligns with sustainable practices in tissue engineering and cultured-meat production, emphasizing its suitability for diverse applications.
Valorization of Dairy and Fruit/Berry Industry By-Products to Sustainable Marinades for Broilers’ Wooden Breast Meat Quality Improvement
Authors: Klementaviciute Jolita , Zavistanaviciute Paulina , Klupsaite Dovile , Rocha João Miguel , Gruzauskas Romas , Viskelis Pranas , El Aouad Noureddine , Bartkiene Elena
Published: April 2024

The study aims to improve the quality of wooden breast meat (WBM) via the use of newly developed marinades based on selected strains of lactic acid bacteria (LAB) in combination with the by-products of the dairy and fruit/berry industries. Six distinct marinades were produced based on milk permeate (MP) fermented with Lacticaseibacillus casei (Lc) and Liquorilactobacillus uvarum (Lu) with the addition of apple (ApBp) and blackcurrant (BcBp) processing by-products. The microbiological and acidity parameters of the fermented marinades were evaluated. The effects of marinades on the microbiological, technical, and physicochemical properties of meat were assessed following 24 and 48 h of WBM treatment. It was established that LAB viable counts in marinades were higher than 7.00 log10 colony-forming units (CFU)/mL and, after 48 h of marination, enterobacteria and molds/yeasts in WBM were absent. Marinated (24 and 48 h) WBM showed lower dry-matter and protein content, as well as water holding capacity, and exhibited higher drip loss (by 8.76%) and cooking loss (by 12.3%) in comparison with controls. After WBM treatment, biogenic amines decreased; besides, the absence of spermidine and phenylethylamine was observed in meat marinated for 48 h with a marinade prepared with Lu. Overall, this study highlights the potential advantages of the developed sustainable marinades in enhancing the safety and quality attributes of WBM.
Development of a Simultaneous Normal-Phase HPLC Analysis of Lignans, Tocopherols, Phytosterols, and Squalene in Sesame Oil Samples
Authors: Yuenyong Jitkunya , Bennett Chonlada , Jiamyangyuen Sudarat , Mahatheeranont Sugunya , Sookwong Phumon
Published: April 2024

The objective of this study was to develop a simultaneous analytical method for the determination of lignans, tocols, phytosterols, and squalene using high-performance liquid chromatography coupled with a diode array and fluorescence detector (HPLC-DAD-FLD). The method employed a VertisepTM UPS silica HPLC column (4.6 × 250 mm, 5 µm) with a mobile phase mixture of n-hexane/tetrahydrofuran/2-propanol. This approach enabled the simultaneous analysis of ten compounds within 22 min. The linear correlation (R2) exceeded 0.9901. The limit of detection (LOD) and limit of quantitation (LOQ) were up to 0.43 µg mL−1 for lignans and tocopherols and up to 326.23 µg mL−1 for phytosterol and squalene. The precision and accuracy of the intra-day and inter-day variation were less than 1.09 and 3.32% relative standard deviations (RSDs). Furthermore, the developed method was applied for the analysis of targeted compounds in twenty-eight sesame oil samples (1775–8965 µg g−1 total lignans, 29.7–687.9 µg g−1 total tocopherols, 2640–9500 µg g−1 phytosterol, and 245–4030 µg g−1 squalene). The HPLC method that has been developed was proven to be a reliable and effective tool for the determination of those functional compounds among sesame oil samples.
Chromatographic and Thermal Characteristics, and Hydrolytic and Oxidative Stability of Commercial Pomegranate Seed Oil
Authors: Siol Marta , Dudek Agnieszka , Bryś Joanna , Mańko-Jurkowska Diana , Gruczyńska-Sękowska Eliza , Makouie Sina , Palani Bharani Kumar , Obranović Marko , Koczoń Piotr
Published: April 2024

The current investigations were aimed at the determination of the hydrolytic and oxidative stability of commercial pomegranate seed oils provided by four different producers, and to assess the oils’ primary quality parameters. During storage, many changes occur in oils that can significantly affect their quality. The oils were tested for acid and peroxide values, fatty acid profile, and their distribution between the sn-1,3 and sn-2 positions of triacylglycerols. The oxidative stability was also determined, and melting curves were plotted for the oils. The analyzed oils were stored for one month in a dark place at refrigerator temperature. Based on the obtained results, it was found that the acid values for most oils did not exceed the permissible level determined by the Codex Alimentarius. However, in all oils, the peroxide value exceeded the permissible level set by the standard EN ISO 3960:2017-03 and the Codex Alimentarius after the one-month storage period. The examined pomegranate seed oils were found to be valuable sources of polyunsaturated fatty acids, especially punicic acid, which was the most abundant fatty acid present in these oils. In all analyzed oils, linoleic acid predominated in the sn-2 position of the triacylglycerols. Pomegranate seed oils did not exhibit good oxidative stability, as the oxidation induction times for all tested oils were very short. The storage period significantly affected the content of the primary oxidation products and oxidative stability of the oils.
Lacticaseibacillus paracasei JS-3 Isolated from “Jiangshui” Ameliorates Hyperuricemia by Regulating Gut Microbiota and iTS Metabolism
Authors: Wu Jiahui , Aga Lvbu , Tang Leimengyuan , Li Houxier , Wang Nan , Yang Li , Zhang Nan , Wang Xiang , Wang Xueyong
Published: April 2024

Background: A diet high in purines can impair the function of the gut microbiota and disrupt purine metabolism, which is closely associated with the onset of hyperuricemia. Dietary regulation and intestinal health maintenance are key approaches for controlling uric acid (UA) levels. Investigating the impacts of fermented foods offers potential dietary interventions for managing hyperuricemia. Methods: In this study, we isolated a strain with potent UA-degrading capabilities from “Jiangshui”, a fermented food product from Gansu, China. We performed strain identification and assessed its probiotic potential. Hyperuricemic quails, induced by a high-purine diet, were used to assess the UA degradation capability of strain JS-3 by measuring UA levels in serum and feces. Additionally, the UA degradation pathways were elucidated through analyses of the gut microbiome and fecal metabolomics. Results: JS-3, identified as Lacticaseibacillus paracasei, was capable of eliminating 16.11% of uric acid (UA) within 72 h, rapidly proliferating and producing acid within 12 h, and surviving in the gastrointestinal tract. Using hyperuricemic quail models, we assessed JS-3’s UA degradation capacity. Two weeks after the administration of JS-3 (2 × 108 cfu/d per quail), serum uric acid (SUA) levels significantly decreased to normal levels, and renal damage in quails was markedly improved. Concurrently, feces from the JS-3 group demonstrated a significant degradation of UA, achieving up to 49% within 24 h. 16S rRNA sequencing revealed JS-3’s role in gut microbiota restoration by augmenting the probiotic community (Bifidobacterium, Bacteroides unclassified_f-Lachnospiraceae, and norank_fynorank_o-Clostridia_UCG-014) and diminishing the pathogenic bacteria (Macrococus and Lactococcus). Corresponding with the rise in short-chain fatty acid (SCFA)-producing bacteria, JS-3 significantly increased SCFA levels (p < 0.05, 0.01). Additionally, JS-3 ameliorated metabolic disturbances in hyperuricemic quails, influencing 26 abnormal metabolites predominantly linked to purine, tryptophan, and bile acid metabolism, thereby enhancing UA degradation and renal protection. Conclusions: For the first time, we isolated and identified an active probiotic strain, JS-3, from the “Jiangshui” in Gansu, used for the treatment of hyperuricemia. It modulates host–microbiome interactions, impacts the metabolome, enhances intestinal UA degradation, reduces levels of SUA and fecal UA, alleviates renal damage, and effectively treats hyperuricemia without causing gastrointestinal damage. In summary, JS-3 can serve as a probiotic with potential therapeutic value for the treatment of hyperuricemia.
Fermenting Acerola (Malpighia emarginata D.C.) and Guava (Psidium guayaba L.) Fruit Processing Co-Products with Probiotic Lactobacilli to Produce Novel Potentially Synbiotic Circular Ingredients
Authors: Araújo Caroliny M. , de Albuquerque Thatyane Mariano R. , Sampaio Karoliny B. , de Oliveira Jordana N. , da Silva Jaielison Yandro P. , Lima Marcos dos S. , Nascimento Yuri M. do , da Silva Evandro F. , da Silva Marcelo S. , Tavares Josean F. , de Souza Evandro L. , de Oliveira Maria Elieidy G.
Published: April 2024

This study evaluated the effects of acerola and guava fruit processing co-products fermented with probiotic Lactobacillus acidophilus LA-05 and Lacticaseibacillus paracasei L-10 on the abundance of different intestinal bacterial groups and microbial metabolic activity during 48 h of in vitro fecal fermentation. Digested fermented fruit co-products increased the relative abundance of beneficial bacterial groups while overall decreasing or maintaining the relative abundance of non-beneficial bacterial groups, suggesting selective stimulatory effects on beneficial bacterial intestinal populations. The fermented co-products stimulated microbial metabolic activity due to decreased pH, sugar consumption, short-chain fatty acid production, phenolic compound and metabolic profile alteration, and high antioxidant capacity during fecal fermentation. Acerola and guava co-products have high nutritional value and bioactive compounds whose fermentation with probiotics improves their potential functionalities. The results show that fermented fruit co-products could induce beneficial changes in the relative abundance of several bacterial groups as well as in the metabolic activity of the human intestinal microbiota. These results highlight their potential as novel and circular candidates for use as synbiotic ingredients.
Machine Learning Model Stability for Sub-Regional Classification of Barossa Valley Shiraz Wine Using A-TEEM Spectroscopy
Authors: Wang Han , Jeffery David W.
Published: April 2024

With a view to maintaining the reputation of wine-producing regions among consumers, minimising economic losses caused by wine fraud, and achieving the purpose of data-driven terroir classification, the use of an absorbance–transmission and fluorescence excitation–emission matrix (A-TEEM) technique has shown great potential based on the molecular fingerprinting of a sample. The effects of changes in wine composition due to ageing and the stability of A-TEEM models over time had not been addressed, however, and the classification of wine blends required investigation. Thus, A-TEEM data were combined with an extreme gradient boosting discriminant analysis (XGBDA) algorithm to build classification models based on a range of Shiraz research wines (n = 217) from five Barossa Valley sub-regions over four vintages that had aged in bottle for several years. This spectral fingerprinting and machine learning approach revealed a 100% class prediction accuracy based on cross-validation (CV) model results for vintage year and 98.8% for unknown sample prediction accuracy when splitting the wine samples into training and test sets to obtain the classification models. The modelling and prediction of sub-regional production area showed a class CV prediction accuracy of 99.5% and an unknown sample prediction accuracy of 93.8% when modelling with the split dataset. Inputting a sub-set of the current A-TEEM data into the models generated previously for these Barossa sub-region wines yielded a 100% accurate prediction of vintage year for 2018–2020 wines, 92% accuracy for sub-region for 2018 wines, and 91% accuracy for sub-region using 2021 wine spectral data that were not included in the original modelling. Satisfactory results were also obtained from the modelling and prediction of blended samples for the vintages and sub-regions, which is of significance when considering the practice of wine blending.
Impulsive Control of Variable Fractional-Order Multi-Agent Systems
Authors: Agarwal Ravi P. , Hristova Snezhana , O’Regan Donal
Published: April 2024

The main goal of the paper is to present and study models of multi-agent systems for which the dynamics of the agents are described by a Caputo fractional derivative of variable order and a kernel that depends on an increasing function. Also, the order of the fractional derivative changes at update times. We study a case for which the exchanged information between agents occurs only at initially given update times. Two types of linear variable-order Caputo fractional models are studied. We consider both multi-agent systems without a leader and multi-agent systems with a leader. In the case of multi-agent systems without a leader, two types of models are studied. The main difference between the models is the fractional derivative describing the dynamics of agents. In the first one, a Caputo fractional derivative with respect to another function and with a continuous variable order is applied. In the second one, the applied fractional derivative changes its constant order at each update time. Mittag–Leffler stability via impulsive control is defined, and sufficient conditions are obtained. In the case of the presence of a leader in the multi-agent system, the dynamic of the agents is described by a Caputo fractional derivative with respect to an increasing function and with a constant order that changes at each update time. The leader-following consensus via impulsive control is defined, and sufficient conditions are derived. The theoretical results are illustrated with examples. We show with an example the leader’s influence on the consensus.
Research on Pattern Dynamics Behavior of a Fractional Vegetation-Water Model in Arid Flat Environment
Authors: Gao Xiao-Long , Zhang Hao-Lu , Wang Yu-Lan , Li Zhi-Yuan
Published: April 2024

In order to stop and reverse land degradation and curb the loss of biodiversity, the United Nations 2030 Agenda for Sustainable Development proposes to combat desertification. In this paper, a fractional vegetation–water model in an arid flat environment is studied. The pattern behavior of the fractional model is much more complex than that of the integer order. We study the stability and Turing instability of the system, as well as the Hopf bifurcation of fractional order α, and obtain the Turing region in the parameter space. According to the amplitude equation, different types of stationary mode discoveries can be obtained, including point patterns and strip patterns. Finally, the results of the numerical simulation and theoretical analysis are consistent. We find some novel fractal patterns of the fractional vegetation–water model in an arid flat environment. When the diffusion coefficient, d, changes and other parameters remain unchanged, the pattern structure changes from stripes to spots. When the fractional order parameter, β, changes, and other parameters remain unchanged, the pattern structure becomes more stable and is not easy to destroy. The research results can provide new ideas for the prevention and control of desertification vegetation patterns.
Feasibility of Principal Component Analysis for Multi-Class Earthquake Prediction Machine Learning Model Utilizing Geomagnetic Field Data
Authors: Qaedi Kasyful , Abdullah Mardina , Yusof Khairul Adib , Hayakawa Masashi
Published: April 2024

Geomagnetic field data have been found to contain earthquake (EQ) precursory signals; however, analyzing this high-resolution, imbalanced data presents challenges when implementing machine learning (ML). This study explored feasibility of principal component analyses (PCA) for reducing the dimensionality of global geomagnetic field data to improve the accuracy of EQ predictive models. Multi-class ML models capable of predicting EQ intensity in terms of the Mercalli Intensity Scale were developed. Ensemble and Support Vector Machine (SVM) models, known for their robustness and capabilities in handling complex relationships, were trained, while a Synthetic Minority Oversampling Technique (SMOTE) was employed to address the imbalanced EQ data. Both models were trained on PCA-extracted features from the balanced dataset, resulting in reasonable model performance. The ensemble model outperformed the SVM model in various aspects, including accuracy (77.50% vs. 75.88%), specificity (96.79% vs. 96.55%), F1-score (77.05% vs. 76.16%), and Matthew Correlation Coefficient (73.88% vs. 73.11%). These findings suggest the potential of a PCA-based ML model for more reliable EQ prediction.