bluuebunny/crossref_metadata_2025 huggingface dataset:
Voltage-Sensing Domain of the Third Repeat of Human Skeletal Muscle NaV1.4 Channel As a New Target for Spider Gating Modifier Toxins
Authors: Myshkin M. Yu. , Paramonov A. S. , Kulbatsky D. S. , Surkova E. A. , Berkut A. A. , Vasilevsky A. A. , Lyukmanova E. N. , Kirpichnikov M. P. , Shenkarev Z. O.
Published: April 2021

Voltage-gated sodium channels (NaV) have a modular architecture and contain five membrane domains. The central pore domain is responsible for ion conduction and contains a selectivity filter, while the four peripheral voltage-sensing domains (VSD-I/IV) are responsible for activation and rapid inactivation of the channel. Gating modifier toxins from arthropod venoms interact with VSDs, influencing the activation and/or inactivation of the channel, and may serve as prototypes of new drugs for the treatment of various channelopathies and pain syndromes. The toxin-binding sites located on VSD-I, II and IV of mammalian NaV channels have been previously described. In this work, using the example of the Hm-3 toxin from the crab spider Heriaeus melloteei, we showed the presence of a toxin-binding site on VSD-III of the human skeletal muscle NaV1.4 channel. A developed cell-free protein synthesis system provided milligram quantities of isolated (separated from the channel) VSD-III and its 15N-labeled analogue. The interactions between VSD-III and Hm-3 were studied by NMR spectroscopy in the membrane-like environment of DPC/LDAO (1 : 1) micelles. Hm-3 has a relatively high affinity to VSD-III (dissociation constant of the complex Kd ~6 M), comparable to the affinity to VSD-I and exceeding the affinity to VSD-II. Within the complex, the positively charged Lys25 and Lys28 residues of the toxin probably interact with the S1S2 extracellular loop of VSD-III. The Hm-3 molecule also contacts the lipid bilayer surrounding the channel.
Удосконалення програмування підготовки кваліфікованих спортсменів у веслуванні
Authors: Русанова Ольга
Published: April 2021

Анотація. Стаття присвячена питанням розробки, формалізації та алгоритмізації програмування й шляхів його практичної реалізації відповідно до системи управління тренувальними і змагальними навантаженнями спортсменів у веслуванні. Мета. Вивчити стан програмування й шляхи його реалізації у веслуванні. Методи. Аналіз, систематизація та узагальнення даних літературних джерел, метод евристичного моделювання. Результати. Основні теоретичні здобутки та розроблені підходи до програмування (аналітико-системний; програмно-цільовий; перспективно прогностичний) посідають важливе місце в арсеналі сучасної теорії та методики спортивної підготовки. Аргументовано, що система програмування відзначається цілісністю і є складно організованою, ієрархічно впорядкованою сукупністю елементів, поєднаних між собою зв’язками, які є результатом взаємного впливу окремих складових. У системі програмування обґрунтовано шляхи практичного застосування та її реалізації у процесі підготовки кваліфікованих спортсменів у веслуванні. Отримані результати дозволили створити алгоритмізовану систему критерій-орієнтованого програмування, що складається з кількох функціональних блоків, а саме: первинний контроль з визначенням індивідуальних параметрів тренувальних навантажень, рівня спеціальної роботоздатності та функціональної підготовленості спортсмена на певному етапі тренувального процесу; формування тренувальної програми для визначеного періоду з урахуванням індивідуальних параметрів навантаження спортсмена; оцінювання результатів контролю та визначення відповідності отриманих показників модельним; корекція завдань тренувальної програми в разі неповної реалізації програми попереднього періоду. В результаті програма піддається корекції, що найчастіше виражається у доповненні завдань нового періоду невиконаними завданнями попереднього; проведення комплексу тренувальних заходів на основі скоригованої програми; оцінка ефективності застосування розробленої програми, аналізу досягнутих критеріїв. Рух циклу закінчується тоді, коли оцінка результатів контролю відповідає встановленим вимогам.

Ключові слова: програмування, веслування, системний підхід, алгоритм програмування.
Організаційно-методичне забезпечення функціонування спортивно-технічних видів спорту в Україні
Authors: Когут Ірина , Маринич Вікторія , Шитікова Єлизавета
Published: April 2021

Анотація. У статті визначено організаційну структуру управління спортивно-технічними видами спорту в Україні.  Ороаналізовано методичне забезпечення діяльності гуртків спортивно-технічного профілю. Мета. Обґрунтування організаційно-методичних засад функціонування спортивно-технічних видів спорту у вітчизняних закладах позашкільної освіти. Методи. Аналіз науково-методичної літератури; синтез та узагальнення; порівняння та зіставлення. Результати. Спортивно-технічні види є складовою неолімпійських видів спорту. У сфері фізичної культури та спорту вони представлені національними федераціями, що координуються Міністерством молоді та спорту України. Зазначені види спорту представлені гуртками, що функціонують у закладах позашкільної освіти, та на державному рівні підпорядковуються Міністерству освіти і науки України. У результаті аналізу методичного забезпечення діяльності гуртків спортивно-технічного профілю встановлено необхідність його вдосконалення з урахуванням основних засад реформування системи позашкільної освіти. Визначено структуру управління спортивно-технічними видами спорту в Україні та координацію їх діяльності між державним та громадським сектором, обґрунтовано шляхи удосконалення їх методичного забезпечення.

Ключові слова: організаційно-методичне забезпечення, спортивно-технічні види спорту.
Carnaval Carioca: ausência de investimentos e sua importância para a gestão municipal
Authors: Cardoso Felipe Sanzi Silva , Correia Isabela Rodrigues , Silva Liliane Aparecida da
Published: April 2021

O presente trabalho tem como objetivo analisar de forma abrangente a problemática da falta de uma política de investimentos no carnaval carioca, no sentido de repensar algumas medidas da administração municipal que comprometem a festividade, podendo afetar diretamente a economia; além de mostrar como o investimento na festividade é tão importante. Apresentam-se o conceito e recursos econômicos advindos da cultura do carnaval, mostrando que o evento tem uma grande importância na captação de receita gerando recursos fundamentais para o gerenciamento municipal. Mostraremos que é preciso uma gestão eficaz e inovadora para que se consiga, não só um retorno dos investimentos aos cofres públicos, assim como a preservação da cultura, o desenvolvimento socioeconômico e o bem-estar da população de uma forma geral. Consequentemente apontaremos as mudanças positivas nas políticas públicas através da captação desses recursos. Sua finalidade é a obtenção de argumentos importantes que explicam o grande sucesso na área econômica e do turismo por causa do carnaval, evidenciando a importância de um programa de ação do governo nos setores e na área cultural.
Pre-Operative Localization of Axillary Lymph Nodes with Tattoo Ink: A Preliminary Report
Authors: Myers Kelly S , Aggarwal Sachin , Oluyemi Eniola T , Habibi Mehran , Ambinder Emily B , Azizi Armina , Sujlana Parvinder , Hung Jessica , Khouri Nagi , Eisner David , Di Carlo Philip A , Cimino-Mathews Ashley M , Camp Melissa S
Published: April 2021

Background: Pre-operative localization options in the axilla are limited. This study aimed to explore the utility of pre-operative localization of axillary lymph nodes using tattoo ink with multidisciplinary correlations.Methods: In this prospective, Institutional Review Board (IRB)-approved study, 19 lymph nodes in 17 patients underwent pre-operative localization with ultrasound-guided injection of Spot tattoo ink. The success rate of intraoperative identification of the tattooed node as well as the frequency in which the tattooed node was also a sentinel node were recorded. Radiologic, surgical and pathologic images were collected. Results: Tattoo ink localization was successful in 16/17 (94.1%) of patients. Tattoo ink did not hinder pathologic evaluation in any cases but was taken up by additional adjacent nodes in 1/17 successful localizations (5.9%). Successful sentinel lymph node biopsy (SLNB) occurred in 13 patients in whom 14 lymph nodes underwent pre-operative tattoo ink localization. Nine of the 14 (64.2%) tattooed lymph nodes were also a sentinel node.Conclusion: In this study, pre-operative localization of axillary lymph nodes with tattoo ink was highly successful In patients undergoing SLNB, a significant number of the tattooed nodes were not sentinel nodes (35.8%), suggesting the importance of targeted lymph node excision in addition to SLNB.
COVID-19-free Surgical Pathways for Breast Cancer Patients
Authors: Grendele Sara , Pellegrini Alice , Bardelli Matteo , Cisternino Giovanni , Melina Marica , Serra Margherita , Taffurelli Mario , Zanotti Simone
Published: April 2021

NA
ESL RURAL PRIMARY STUDENTS’ PERFORMANCE AND PERCEPTIONS OF LEARNING ENGLISH VOCABULARY THROUGH GAMES
Authors: Salim Sulaihah , Abd Halim Nisha , Syed Adnan Sharifah Nurul Izati , Mat Zin Siti Fatimah , Nik Din Nik Nur Fathiha , Yaacob Aizan
Published: April 2021

This action research investigated the use of games to boost ESL rural
primary pupils’ motivation to learn English vocabulary. 18 Year 6
pupils from a rural school in Gua Musang Kelantan were selected
by using convenient sampling. Multiple methods such as pre–test,
post–test, observation, and interviews with teachers and pupils were
used for data collection. This paper highlighted pupils’ voices of the
effectiveness of vocabulary learning as the result of the pre and posttest
showed a significant difference in which pupils have acquired
the new words effectively and there was an improvement after having
a game-based lesson. The findings indicated that the pupils showed
significant improvement in their vocabulary scores after using games.
Students reported that learning vocabulary by using games was fun
and interesting, as it increased their motivation to learn the English
language, helped them to memorise the vocabulary items faster, and
increased their interaction in class. Furthermore, the competitive and
repetitive nature of the game helped in vocabulary retention.
EFIKASI KENDIRI GURU SEBAGAI MEDIATOR TERHADAP HUBUNGAN ANTARA IKLIM SEKOLAH DAN KOMITMEN GURU
Authors: Mat Jusoh Musliza , Ismail Siti Noor , Abdullah Anis Salwa
Published: April 2021

The Ministry of Education Malaysia aims to develop an educational system that is capable in improving achievement and innovation. This goal requires high commitment and precision from all parties, especially teachers that play the main role in educational institutions. Thus, a positive school climate will produce high-quality human capital, while self-efficacy acts as a driving force to pursue the goals. The effectiveness of these two elements enhances the commitment of teachers in order to ensure the success of educational institutions. This study was developed to examine the relationship between school climate and teacher commitment, as well as the indirect relationship through self-efficacy as a mediator construct. Teachers were selected randomly from 18 high-achievement secondary schools in Kelantan, with a total of 360 respondents. A cross-sectional survey method was applied to collect data. The questionnaire consisted of 44 items. The IBM SPSS software was used to analyze the correlation and regression tests. The Pearson correlation analysis confirmed positive and significant relationships between the three variables, namely the weak correlation for school climate and commitment (r=.24, p<.01), as well as between school climate and self-efficacy (r=.23, p<.01). In contrast, the correlation was high between selfefficacy and commitment (r=.85, p<.01). Regression analysis for the effect of school climate on teacher commitment showed that R2=.06, p<.01 was significant. The beta value showed that the school’s climate contributed β=.24 (24%), significantly affecting commitment. Multiple regression analysis of school climate and self-efficacy on teacher commitment confirmed that R2=.72, p<.01 was significant. However, beta values showed that school climate contribution decreased to 0.05 (5%) and did not significantly affect commitment as the self-efficacy predictor was included in the regression model. The beta value showed that self-efficacy contributed 0.84 (84%), significantly influencing commitment and acting as a full mediator variable for school climate and teachers’ commitment. The finding of this study proposes significant school climate relationships to enhance teachers’ self-efficacy and commitment. This study also contributes significantly to the theory and the extension of knowledge by explaining the role of school climate in enhancing teachers’ commitment, as well as highlighting the mediator role of self-efficacy that has a significant effect on teacher commitment as well. Therefore, the school administrators should provide a better, condusive school climate to encourage higher self-efficacy among teachers, through self-confidence in group guidance, student assignments, innovative education and attitude towards homework. As a result, teachers could have more opportunity to develop their commitment to schools.
Integration and Local Finnish Governments: Local Turn or State Responsibility?
Authors: Kettunen Pekka
Published: April 2021

Local governments have become more important in the integration of immigrants, constituting a local turn in integration policy. However, the empirical evidence is still limited and variation between countries is presumably high. The present paper analyses the role played by Finnish local governments in the integration process, with municipal governments being highly autonomous from a general European perspective. The paper delineates the basic features of local integration policy, i.e. who does what, and thereafter it assesses the integration plans of local governments and how they justify the current policy. A closer look at Finnish integration policy, however, suggests that state administration actually has a strong role and that most resources are being directed to integration courses administered by the labour administration of the state. What remains for the local governments is to foresee that their services are suitable for immigrants, too. There is an asymmetry between local governments, though, as immigrants mostly live in larger cities, and half of them in the capital city area. While local governments are obliged to assess their integration policies, such assessments mostly deal with outputs and the feedback of service users. In sum, only a weak local turn seems to be emerging in Finland.
Bajapuik Tradition the Traditional Marriage in Minangkabau
Authors: Anisa Hafizatul , Aman Aman , Kumalasari Dyah
Published: April 2021

Bajapuikis a tradition of the Minangkabau people native to the Padang Pariaman area which is carried out in a traditional marriage process. Bajapuik is the custom of the Minang community in respecting the position of men from Padang Pariaman but not detrimental to women as the biological bundo in the rumah gadang. Bajapuik has become a mandatory tradition at wedding ceremonies in Padang Pariaman by picking up / buying the groom using money whose nominal value is determined by means of family congregation and traditional leaders. Writing this article aims to describe the cultural customs of marriage in Minangkabau with a focus on the study of the Bajapuik tradition in Padang Pariaman. The method used in writing this scientific article is a qualitative method with a descriptive approach.
PREDIKSI KELANCARAN PEMBAYARAN CICILAN CALON DEBITUR DENGAN METODE K-NEAREST NEIGHBOR
Authors: Rizky Sri Ayu , Yesputra Rolly , Santoso Santoso
Published: April 2021

Abstract: In this research, a prediction system has been successfully developed to predict whether or not a prospective money borrower will run smoothly. Prospective borrowers who will borrow, some of the data that meet the criteria will be inputted by the office clerk into a prediction application system interface to be processed using the Data Mining method, namely the K-Nearest Neighbor Algorithm with the Codeigniter programming language 3. The results of the Euclidean calculation process are based on predetermined criteria Between training data (training) to testing data (test) will be displayed with a table that has been sorted from smallest to largest containing 9 closest neighbors according to the K value that has been determined, namely 9. The nine neighbors will be taken the dominant category. This dominant category can be used as a guideline that makes it easier for the leader to make a decision on the next borrower.            Keywords: Data Mining; Euclidean; K-Nearest Neighbor; Prospective Borrowers;  Abstrak: Dalam penelitian ini telah berhasil dibuat sebuah sistem prediksi untuk memprediksi lancar atau tidak lancarnya seorang calon peminjam uang. Calon peminjam uang yang akan meminjam, sebagian datanya yang memenuhi kriteria akan diinputkan petugas kantor ke dalam sebuah interface sistem aplikasi prediksi untuk diolah menggunakan metode Data Mining yaitu Algoritma K-Nearest Neighbor dengan bahasa pemrograman Codeigniter 3. Hasil proses perhitungan Euclidean berdasarkan kriteria yang sudah ditentukan antara data training (latih) ke data testing (uji) tersebut akan ditampilkan dengan sebuah tabel yang sudah diurutkan dari yang terkecil ke terbesar berisi 9 tetangga terdekat sesuai dengan nilai K yang sudah ditentukan yaitu 9.  Sembilan tetangga tersebut akan diambil kategori yang dominan. Kategori yang dominan tersebut bisa dijadikan suatu pedoman yang memudahkan pimpinan dalam mengambil sebuah keputusan kepada calon peminjam selanjutnya. Kata kunci: Debitur; Data Mining; Euclidean; K-Nearest Neighbor
SISTEM INFORMASI GEOGRAFIS PEMETAAN SEKOLAH MENENGAH KEJURUAN DI KABUPATEN LABUHANBATU MENGGUNAKAN WEBGIS
Authors: Zahara Lisma , Munthe Ibnu Rasyid , Ritonga Ali Akbar
Published: April 2021

Abstract: Labuhanbatu Regency is a district that has many Vocational High Schools (SMK) which are very attractive to the community. Because each Vocational High School has its own vocational fields, for example Health, Automotive, Offices, and others. The existence of this Geographic Information System makes it easy for users to find Vocational High School routes. Vocational High Schools are schools that make students or prospective students even more skilled. In making this Geographic Information System requires Systems Development Life Cycle to analyze gradually, and Webgis to display maps as well as GPS to ascertain the location of the earth's surface and use UML design such as activity diagrams, sequence diagrams, and usecase. There is also a waterfall method in making this Geographic Information System such as its analysis, implementation, design, maintenance and also the testing phase. The Testing Phase uses the BlackBox to test the system whether it is running well or not and is suitable for use by users. Keywords: BlackBox Testing; Geographic Information System; Google Maps; GPS; UML; Vocational High School  Abstrak: Kabupaten Labuhanbatu termasuk kabupaten yang memiliki banyak Sekolah Menengah Kejuruan (SMK) yang sangat diminati masyarakat. Karena setiap SMK memiliki kejuruan masing-masing dalam bidangnya misalkan Kesehatan, Otomotif, Perkantoran, dan lain-lain. Adanya Sistem Informasi Geografi ini memudahkan para pengguna untuk mencari rute Sekolah Menengah Kejuruan. Sekolah Menengah Kejuruan adalah sekolah yang menjadikan para siswa atau calon siswa lebih trampil lagi. Dalam pembuatan Sistem Informasi Geografi ini memerlukan Systems Development Life Cycle menganalisis secara bertahap, dan Webgis untuk menampilkan peta juga GPS untuk memastikan letak permukaan bumi dan menggunakan Perancangan UML seperti activity diagram, sequence diagram, dan usecase. Terdapat juga Metode waterfall dalam membuat Sistem Informasi Geografi ini seperti analisanya, implementasi, desain, pemeliharaan dan juga tahap pengujian. Tahap Pengujian menggunakan BlackBox untuk menguji Sistem apakah berjalan baik atau tidak dan sudah layak digunakan bagi pengguna. Kata kunci:  Sekolah Menengah Kejuruan; GPS; Peta Google; Sistem Informasi Geografi; Uji  BlackBox; UML
Çocuk İstismarı ve İhmalini Anlamak: Ekolojik Bir Yaklaşım
Authors: Türkkan Tuğba , Odacı Hatice , Bülbül Kenan
Published: April 2021

Çocuk istismarı ve ihmali, özellikle son yıllarda ruh sağlığı alanında çalışan profesyoneller için bir endişe kaynağı olmuştur. İlgili literatürde çocuk istismarını anlamak, istismara yol açan risk faktörlerini tanımlamak ve çocuğa yönelik istismarı önlemek için çeşitli yaklaşımlar bulunmaktadır. Ne yazık ki, bu yaklaşımların genellikle çocuk istismarı gibi karmaşık bir sosyal sorunu anlamak ve ortadan kaldırmak için yetersiz kaldığı görülmektedir. Son yıllarda, çocuk istismarının etiyolojisi ve süreçlerini açıklamak üzere daha kapsamlı bir yaklaşım olan ekolojik modelin ilgili literatüre dahil edildiği dikkat çekmektedir. Ekolojik yaklaşıma göre çocuk istismarı, tek başına hareket eden bir etkinin değil, çok sayıda faktörün etkileşimi sonucunda meydana gelir. İstismara yönelik araştırma ve uygulamaları yürütmek için sistematik bir çerçeve sağlayan bu model, çocuk istismarı ve ihmalini anlamaya katkı sağlayan en kapsamlı modeldir. Bu çalışmada çocuk istismarı olgusu ekolojik model çerçevesinde değerlendirilmekte ve bu kapsamda alanda çalışan uzmanlara makro, mezo ve mikro düzeyde pratik öneriler sunulmaktadır.
Yaşlanmayla İlgili Kalıp Yargı Tehdidinin Bellek Performansı Üzerindeki Etkisi
Authors: Özmen Gülpek Duygu , Güleç Hayriye , Tekman Hasan Gürkan
Published: April 2021

Bu çalışmanın amacı yaşlanmayla ilgili kalıp yargı tehdidinin 55-70 yaş arasındaki kişilerin bellek performansı üzerindeki etkisini incelemektir. Katılımcılar negatif kalıp yargı tehdidi koşulu, pozitif kalıp yargı tehdidi koşulu ve kontrol koşulu olmak üzere üç deneysel koşula rastgele olarak atanmışlardır. Manipülatif metinlerin sunulmasının ardından kalıp yargı tehdidinin bellek performansı üzerindeki etki süresini gözleyebilmek için katılımcılara art arda iki bellek görevi verilmiştir. Çalışmanın bulguları kalıp yargı tehdidi ile ilgili metinlere maruz kalan katılımcıların bellek performansının gruplar arasında anlamlı şekilde birbirinden farklılaşmadığını göstermiştir. Katılımcıların ardışık iki kelime listesinde hatırladıkları kelime sayıları benzerdir. Katılımcıların hatırladıkları kelime sayısı ile cinsiyetleri ve eğitim düzeyleri arasında anlamlı ilişkiler bulunmuştur. Kadınlar erkeklere göre, üniversite ve üzeri eğitim görenler, üniversite altı eğitim görenlere göre daha iyi bellek performansı göstermişlerdir.
Análise do sistema de patentes no Brasil, no espaço temporal de 2000 a 2018
Authors: Pereira Gyzah Amui Barros , Queiroz Lucas de Aquino Amorim , Martins Luciana da Costa e Silva , Aguiar Maristela Mendes , Mendes Naiara Rosa , Gaydeczka Beatriz , Okura Mônica Hitomi , Granato Ana Claudia , Malpass Geoffroy Roger Pointer
Published: April 2021

Esse trabalho teve como objetivo analisar o sistema de patentes no Brasil, no espaço temporal de 2000 a 2018. Os procedimentos metodológicos consistiram em levantamento de dados das bases do Instituto Nacional da Propriedade Industrial (INPI) e da Organização Mundial da Propriedade Intelectual (World Intellectual Property Organization – WIPO), os quais serviram de apoio para a extração das informações para esta pesquisa. O trabalho apresentou uma análise da evolução da propriedade intelectual no Brasil, além de análise comparativa com outros países. Para a pesquisa foram utilizados os modelos de avaliação descritiva, quantitativa e transversal. A partir dos resultados obtidos verificou-se que o percentual dos depósitos de patentes é significativamente maior em relação às concessões; as concessões para os não-residentes são maiores do que para os residentes; dos residentes, as Universidades e as Instituições Públicas são os principais impulsionadores de patentes. O Brasil está entre os maiores escritórios de patentes do mundo. Ainda foi possível observar que a China possui o maior desenvolvimento e crescimento de patentes entre os anos de 2004 a 2018. Como conclusão cabe destacar que o Brasil, ao longo do período analisado, conquistou avanços em relação à Propriedade Intelectual, por meio do fortalecimento das políticas internas, a participação de tratados internacionais, investimentos de multinacionais, e Universidades e Instituições Públicas produtivas, os mecanismos efetivos para essa conquista.
Potencialidades e fragilidades do clima de segurança do paciente: Scoping review
Authors: Barboza Ana Raquel Campos de Almeida , Figueiredo Karla Crozeta , Miranda Fernanda Moura D’Almeida , Reis Cláudia Tartaglia , Silva Thaiane Almeida , Siqueira Carolina Poite de , Figueredo Alda Souza
Published: April 2021

Objetivos: mapear os estudos que utilizaram o Safety Attitudes Questionnaire em unidades críticas hospitalares; identificar os pontos fortes e fracos do clima de segurança do paciente por meio dos domínios do instrumento e relacionar as estratégias de fortalecimento para a segurança do paciente. Método: Scoping Review realizada nas bases de dados Literatura Latino-Americana e do Caribe em Ciências da Saúde, Public/Publisher Medical Literature Analysis and Retrievel System Online, Cumulative Index to Nursing and Allied Health Literature, Scopus; Web of Science, Joanna Briggs Institute Library of Systematic Reviews e outras fontes com estudos publicados entre 2006 - 2020 que utilizaram o instrumento Safety Attitudes Questionnaire. Resultados: Incluídos 25 estudos que avaliaram o clima de segurança no Centro Cirúrgico e Unidade de Terapia Intensiva. Evidenciou-se um clima de segurança fraco com escores abaixo do recomendado. Na avaliação dos escores dos seis domínios do instrumento, os dados dos estudos incluídos apontaram a “Percepção da gerência” como ponto mais fraco e a “Satisfação no Trabalho” foi o único item que apresentou escore forte. Estratégias para melhorias da qualidade da assistência e fortalecimento da segurança do paciente foram sugeridas nos estudos, como educação em serviço e implantação de sistemas de notificação de eventos adversos. Conclusão: Este estudo permitiu reconhecer os pontos fortes e fracos mapeados na literatura, o que favorece o planejamento para construção de novas práticas assistenciais em saúde, na qualidade da assistência e no desenvolvimento organizacional.
A cooperação judiciária como técnica de gestão processual para a modificação de competência visando evitar decisões conflitantes
Authors: Costa Rosalina Moitta Pinto da
Published: April 2021

O trabalho analisa a cooperação judiciária como técnica de gestão processual para a modificação de competência a fim de evitar decisões conflitantes. Utilizando o método dedutivo com revisão doutrinária como opção metodológica, analisa-se a cooperação judiciária como técnica capaz de dar concretude ao princípio da eficiência, pela modificação de competência. Como os atos de cooperação, permitindo o diálogo institucional entre juízes e órgãos do Poder Judiciário para responder às situações concretas, caracterizam-se pela falta proposital de um regramento predefinido pelo legislador, conclui-se que os atos de cooperação que visam a reunião de processos, ainda que estejam autorizados pelo sistema processual, importam em uma nova forma de modificação de competência como atos de gestão processual que são, para respeitar o princípio da eficiência processual.
Tidal stream use by black guillemots Cepphus grylle in relation to a marine renewable energy development
Authors: Johnston DT , Furness RW , Robbins AMC , Tyler GA , McIlvenny J , Masden EA
Published: April 2021

Seabirds that forage within tidal streams may be vulnerable to collision or habitat change due to tidal stream turbines. The black guillemot Cepphus grylle is considered to be the seabird species most at risk from tidal stream turbines in UK waters. Using GPS tracking of adult breeding black guillemots, carried out on the island of Stroma, Caithness, in 2016 and 2017, we examined habitat use within the Inner Sound of the Pentland Firth in relation to the MeyGen tidal lease area (MTLA). We found foraging areas of black guillemots within the Inner Sound to be influenced by tidal velocity and seafloor depth. The velocities and depths which black guillemots selected while foraging within a 1 km boundary of the lease area significantly differed from those concurrently occurring within the MTLA (velocities: foraging = 0.79 m s-1, MTLA = 1.57 m s-1; depths: foraging = 24.55 m, MTLA = 32.09 m). This disparity between the used habitat and the conditions within the MTLA may indicate a reduced potential for interactions with turbines. The potential for collision with turbine blades is further reduced by black guillemots predominantly associating with mean tidal velocities slower than the 1 m s-1 cut-in speeds of the MeyGen turbines. However, as more turbines are constructed within the lease area (up to 398 turbines proposed), habitat and hydrodynamic conditions may be altered to become more suitable for foraging, so monitoring black guillemot foraging behaviour post-construction is strongly recommended.
Gene flow across a major biogeographic barrier is not increasing under climate change for the barnacle Catomerus polymerus
Authors: Ayre DJ , Rosser N
Published: April 2021

On the south-east coast of Australia, the impermeability of the Southeast Australian Biogeographic Barrier (SEABB) limits the dispersal of many intertidal taxa, but current flow across the SEABB is expected to be increasing under climate change. The surf barnacle Catomerus polymerus has extensive populations on either side of the barrier that both mitochondrial cytochrome c oxidase subunit I (mtCOI) sequence data and surveys using 4-6 microsatellite loci imply have been separated for millennia (>167000 yr). Nevertheless, these data sets (based on collections from 2005-2012) also imply that there has been some recent dispersal across the SEABB. If trans-barrier migration is now possible, then in the absence of selection the differentiation of lineages should be eroded. Next-generation sequencing provides the opportunity to both better quantify levels of population differentiation attributable to the SEABB and to determine if there is regional selection. Our data (3801 single nucleotide polymorphisms) for individuals collected in 2019 within 3 eastern and 4 western sites support earlier reports of low population differentiation within eastern and western regions (FST < 0.021) and the strong regional separation of eastern and western lineages (FRT = 0.23). Moreover, in contrast to earlier studies, we did not detect any putative migrants, with all individuals assigning most strongly to their sampling region. Most strikingly, analysis using BAYESCAN implied that 47 loci (1.24%) of our surveyed loci show evidence of significant regional diversifying selection, which implies that even if C. polymerus larvae are able to cross the barrier, they will be strongly disfavoured by selection.
“Our eyes are sentinels unto our judgments”: Embodied Perception in The Changeling
Authors: Smith M. Burdick
Published: April 2021

This essay argues that Thomas Middleton and William Rowley's The Changeling (1622) draws on debates about sense perception in the period to interrogate the effects of dramatic representation. After a brief overview of early modern perceptual theory, this essay demonstrates that the play's villain, De Flores, manipulates other characters’ perception through language. In fact, De Flores uses theatrical language to manipulate how other characters perceive their environment, indicating the theater's ability to manipulate audiences. By affecting how characters perceive, De Flores affects other characters’ ability to process and react to their environment, which impedes their judgment. The essay argues that much of The Changeling's dramatic action unfolds through a conflict between two models of perception—presentational and representational—that undergird much of the play's dramatic conflict. In the play, pervasive anxiety about judgment, particularly how perception affects judgment, is structured around the distinction between these two models of perception. Considering the play alongside representational and presentational models indicates how early modern dramatists engage with intellectual theories to consider how representation works and how spaces are experienced. In this way, the theater refracts and dramatizes theories about perception.
POLITRAUMA DURING COVID-19 PANDEMIC: AN INCREASING INCIDENCE OF DOMESTIC VIOLENCE
Authors: Socea B. , Bogaciu Cristiana , Nica Anca A. , Smaranda A.C. , Ciobotaru V.P. , Crăciun Roxana I. , Carâp A.C. , Slavu I. , Tulin A. , Alecu L. , Badiu C.D. , Constantin V.D.
Published: April 2021

The Covid-19 pandemic changed certain social habits and practices. The pandemic also changed the profile of polytrauma patients who presented in the emergency room. The etiology of polytraumas has undergone changes in the context of social distancing. While road accidents, falls and physical assaults, including gunshot trauma on the streets have decreased, we are confronting with an alarming increase in domestic violence. We analyzed the etiology of polytraumas from a period of 3 months that overlapped with the pandemic and compared it with the similar periods of the previous 3 years. We found an increase of about 4 times the incidence of physical aggression through domestic violence.
Psicoanálisis de la Corrupción - Política y ética en el Perú contemporáneo
Authors: Morales-Soto Nelson Raúl
Published: April 2021

Resumen
GRAMMAR-NAZI ANALYSIS IN ENGLISH LEARNING PROCESS AMONG MILLENIALS IN INDONESIA
Authors: Soehendro Eunike Imanuela , Septiani Ika Wuri , Zhafarina Zhafira , Jumanto Jumanto
Published: April 2021

Social media, which was originally used to communicate with other people via online, has begun to be used to exchange knowledge so that it makes it easy for many people to learn more flexibly and without boundaries. Indonesian people are more motivated to learn and practice English through social media, considering that the ability to speak English is a special value when applying to job. However, the main function of social media itself, namely the freedom of expression and opinion, is also an inhibiting factor in learning English. Lots of social media users seem to be less wise in their opinions, especially on grammar issues and do not hesitate to criticize grammatical errors in English posts. The term grammar-nazi is usually attached to these people who tend to correct the grammar. This is the main focus of researchers to conduct grammar-nazi analysis in the process of learning English among millennials in Indonesia which is carried out online. Our research process includes data collection through observation, open coding techniques, axial coding, selective coding, synthesis of results, drawing conclusions, and providing suggestions. Some opinions such as Lauren & Connie (2005) and Mohd Amin et al.  (2016) in their research provides an overview of the responses of users who showed a positive  with this phenomenon. Meanwhile, research by James E. Carroll (2016) and Sherman & Jaroslav (2014) shows a negative response disagreeing with this phenomenon. The results of this study are expected to be able to provide a sufficient account on grammar-nazi phenomenon in the process of learning English among millennials in Indonesia.
FUNCTIONS OF CONVERSATIONAL IMPLICATURE IN TV SHOW “F.R.I.E.N.D.S”: PRAGMATICS APPROACH
Authors: Cristina Vivian , Afriana Afriana
Published: April 2021

The aimed of this research is to analyze the function of the conversational implicature found in a famous American TV show, named F.R.I.E.N.D.S. The researcher used the conversational implicature theory by Grice (1975) to identify the utterance and the speech acts theory by Searle (1976) to categorize the function. The design of this research is qualitative research. The researcher used the interview method by Sudaryanto (2015) to collect data, the pragmatic identity method by Sudaryanto (2015) to analyze the data, and used the informal presentation method by Sudaryanto (2015) to present the research result. The researcher collected ten data that contain conversational implicature, and two of the data categorized as representatives/ assertives, three data categorized as directives, two data categorized as commissives, two data categorized as expressive, and the remaining data categorized as declarations. The result concludes that out of the ten analyzed data, directives is the most used speech acts in the TV show.
HEROINE AND PRINCESS: WOMEN IMAGE PORTRAYED IN SELECTED DISNEY’S STORIES
Authors: Daulay Resneri
Published: April 2021

The purpose of this study is to revealed the women image portrayed in selected Disney’s stories. There are several stories of Disney’s movie adaptation that are used as object in this research. There are Sleeping Beauty (Disney, 2014), Snow White (Brothers Grimm, 1812), Beauty and The Beast (Gabrielle-Suzanne Barbot, 1740), Cinderella (Giambattista Basile, 1634), Mulan (Guo Maoqian, 1992), and Brave (Disney, 2012).  In addition, the aim of this research is also to identify the characteristics of female characters in selected Disney’s stories related to the Heroine and Princess attitude. This study used qualitative research; descriptive qualitative methods are used to analyze data. This study used three main concepts of semiotics theory by Roland Barthes, there are meaning of denotation, connotation, and myth. This study also applied feminism approach accordance to women attitude as heroine and princess. In this study, the researcher found some results. First, this study indicated the women image portrayed in Disney’s stories contains two images, they are women as heroine and princess. Women image as heroine is revealed in Beauty and the Beast, Mulan and Brave. Meanwhile, women as princess is portrayed in Sleeping Beauty, Snow White and Cinderella. Then, there are several characteristics found in Disney’s stories that represent the woman’s character as heroine and princess. They are from the feminism such as submissive, kind and gentle, domestic role, damsel in distress, emotional balance, craving for freedom (independent and brave), willing to sacrifice and has ability to stand up to the antagonist.
STRATEGIES OF POLITENESS USED BY THE AMBONESE IN REFUSING INVITATIONS
Authors: Azwan Azwan
Published: April 2021

The aims of this research is to determine the strategies of politeness refusal used by the Ambonese in refusing invitations, and to determine the effects of social relationship towards the strategies of politeness used by the Ambonese in refusing invitations, The research used a descriptive qualitative approach. The data were collected from direct observation by recording the conversations in any situation between the interlocutors. The data were analyzed based on Yassi’s six models of politeness system, and classifies the strategies of politeness which are formulated by Brown and Levinson, The results show that Ambonese speakers mostly use long refusal sequences, In the interaction between the strangers, Ambonese speakers tend to use bald on record strategy such as direct refusal as a head act of refusals sequence, In intimate relationship, Ambonese speakers tend to combine addressed form, reason, promise, joke or offer new solution as positive politeness strategy. In hierarchal relationship, Ambonese speakers tend to use negative politeness strategies such as give deference and expressing apology by an inferior to a superior person, Culturally, Ambonese people tend to be influenced by Pela gandong to maintain the solidarity in responding and addressing.
A Custom-Made Device for Reproducibly Depositing Pre-metered Doses of Nebulized Drugs on Pulmonary Cells in vitro
Authors: Horstmann Justus C. , Thorn Chelsea R. , Carius Patrick , Graef Florian , Murgia Xabier , de Souza Carvalho-Wodarz Cristiane , Lehr Claus-Michael
Published: April 2021

The deposition of pre-metered doses (i.e., defined before and not after exposition) at the air–liquid interface of viable pulmonary epithelial cells remains an important but challenging task for developing aerosol medicines. While some devices allow quantification of the deposited dose after or during the experiment, e.g., gravimetrically, there is still no generally accepted way to deposit small pre-metered doses of aerosolized drugs or pharmaceutical formulations, e.g., nanomedicines. Here, we describe a straightforward custom-made device, allowing connection to commercially available nebulizers with standard cell culture plates. Designed to tightly fit into the approximately 12-mm opening of either a 12-well Transwell® insert or a single 24-well plate, a defined dose of an aerosolized liquid can be directly deposited precisely and reproducibly (4.8% deviation) at the air–liquid interface (ALI) of pulmonary cell cultures. The deposited dose can be controlled by the volume of the nebulized solution, which may vary in a range from 20 to 200 μl. The entire nebulization-deposition maneuver is completed after 30 s and is spatially homogenous. After phosphate-buffered saline (PBS) deposition, the viability and barrier properties transepithelial electrical resistance (TEER) of human bronchial epithelial Calu-3 cells were not negatively affected. Straightforward in manufacture and use, the device enables reproducible deposition of metered doses of aerosolized drugs to study the interactions with pulmonary cell cultures grown at ALI conditions.
Long-Read Sequencing to Unravel Complex Structural Variants of CEP78 Leading to Cone-Rod Dystrophy and Hearing Loss
Authors: Ascari Giulia , Rendtorff Nanna D. , De Bruyne Marieke , De Zaeytijd Julie , Van Lint Michel , Bauwens Miriam , Van Heetvelde Mattias , Arno Gavin , Jacob Julie , Creytens David , Van Dorpe Jo , Van Laethem Thalia , Rosseel Toon , De Pooter Tim , De Rijk Peter , De Coster Wouter , Menten Björn , Rey Alfredo Dueñas , Strazisar Mojca , Bertelsen Mette , Tranebjaerg Lisbeth , De Baere Elfride
Published: April 2021

Inactivating variants as well as a missense variant in the centrosomal CEP78 gene have been identified in autosomal recessive cone-rod dystrophy with hearing loss (CRDHL), a rare syndromic inherited retinal disease distinct from Usher syndrome. Apart from this, a complex structural variant (SV) implicating CEP78 has been reported in CRDHL. Here we aimed to expand the genetic architecture of typical CRDHL by the identification of complex SVs of the CEP78 region and characterization of their underlying mechanisms. Approaches used for the identification of the SVs are shallow whole-genome sequencing (sWGS) combined with quantitative polymerase chain reaction (PCR) and long-range PCR, or ExomeDepth analysis on whole-exome sequencing (WES) data. Targeted or whole-genome nanopore long-read sequencing (LRS) was used to delineate breakpoint junctions at the nucleotide level. For all SVs cases, the effect of the SVs on CEP78 expression was assessed using quantitative PCR on patient-derived RNA. Apart from two novel canonical CEP78 splice variants and a frameshifting single-nucleotide variant (SNV), two SVs affecting CEP78 were identified in three unrelated individuals with CRDHL: a heterozygous total gene deletion of 235 kb and a partial gene deletion of 15 kb in a heterozygous and homozygous state, respectively. Assessment of the molecular consequences of the SVs on patient’s materials displayed a loss-of-function effect. Delineation and characterization of the 15-kb deletion using targeted LRS revealed the previously described complex CEP78 SV, suggestive of a recurrent genomic rearrangement. A founder haplotype was demonstrated for the latter SV in cases of Belgian and British origin, respectively. The novel 235-kb deletion was delineated using whole-genome LRS. Breakpoint analysis showed microhomology and pointed to a replication-based underlying mechanism. Moreover, data mining of bulk and single-cell human and mouse transcriptional datasets, together with CEP78 immunostaining on human retina, linked the CEP78 expression domain with its phenotypic manifestations. Overall, this study supports that the CEP78 locus is prone to distinct SVs and that SV analysis should be considered in a genetic workup of CRDHL. Finally, it demonstrated the power of sWGS and both targeted and whole-genome LRS in identifying and characterizing complex SVs in patients with ocular diseases.
Cryptococcal Virulence in Humans: Learning From Translational Studies With Clinical Isolates
Authors: de Sousa Herdson Renney , Frazão Stefânia de , Oliveira Júnior Getúlio Pereira de , Albuquerque Patrícia , Nicola André Moraes
Published: April 2021

Cryptococcosis, an invasive mycosis caused by Cryptococcus spp, kills between 20% and 70% of the patients who develop it. There are no vaccines for prevention, and treatment is based on a limited number of antifungals. Studying fungal virulence and how the host responds to infection could lead to new therapies, improving outcomes for patients. The biggest challenge, however, is that experimental cryptococcosis models do not completely recapitulate human disease, while human experiments are limited due to ethical reasons. To overcome this challenge, one of the approaches used by researchers and clinicians is to: 1) collect cryptococcal clinical isolates and associated patient data; 2) study the set of isolates in the laboratory (virulence and host-pathogen interaction variables, molecular markers); 3) correlate the laboratory and patient data to understand the roles fungal attributes play in the human disease. Here we review studies that have shed light on the cryptococcosis pathophysiology using these approaches, with a special focus on human disease. Isolates that more effectively evade macrophage responses, that secrete more laccase, melanize faster and have larger capsules in the cerebrospinal fluid are associated with poorer patient outcomes. Additionally, molecular studies have also shown that cryptococcal clades vary in virulence, with clinical impact. Limitations of those studies include the use of a small number of isolates or retrospectively collected clinical data. The fact that they resulted in very important information is a reflection of the impact this strategy has in understanding cryptococcosis and calls for international collaboration that could boost our knowledge.