bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding Blame Politics in China's Decentralized System of Environmental Governance: Actors, Strategies and Context
Authors: Ran Ran
Published: August 2017

Decentralized environmental governance theory suggests that decentralization can produce better environmental performance mainly because lower-level governments are closer to the people and environmental issues and are considered more legitimate than the national government. However, China's decentralized system of environmental governance has been often regarded as a key factor in creating pollution problems rather than in solving them. To explain this puzzle, this article, using Blame Avoidance Behaviour in government theory as a theoretical framework, examines how blame avoidance behaviour shapes China's decentralized system of environmental governance from three perspectives: first, actors and the chain of blame shaped by the hierarchical power structure among environmental policymakers and implementers; second, the strategies of discursive domination and decentralization for blaming environmental problems on local officials; and lastly, the contextual factor of “hierarchical governmental trust.” Drawing on documentary discursive analysis and extensive fieldwork, this article suggests that the dysfunction of China's decentralized environmental governance structure may in fact be an outcome of a blame-shifting game between central and local governments.
China's War on Air Pollution: Can Existing Governance Structures Support New Ambitions?
Authors: Wong Christine , Karplus Valerie J.
Published: August 2017

Unprecedented and highly visible degraded air quality in China's urban centres has prompted a step change in central government control efforts in recent years. This “War on Air Pollution” has included a mixture of administrative controls, regulatory clampdowns, economic incentives and public education campaigns. A critical constraint on how policies are designed and implemented is the central government's capacity to access accurate cost information, and monitor, evaluate and enforce the policies at subordinate levels of government. We examine in detail the directives and arrangements that underpin China's “War on Air Pollution” at the provincial level, taking Hebei province as a case study. Located upwind of Beijing, Hebei's heavy industries have been a particular focus of the environmental policies. The current approach, which requires highly specific and costly local actions, yet allocates funds centrally, suffers from misaligned incentives and does not address longstanding weaknesses in local policy monitoring, evaluation and enforcement.
The Black American Jacobins: Revolution, Radical Abolition, and the Transnational Turn
Authors: Dahl Adam
Published: August 2017

While scholars of African American political thought have done a remarkable job centering focus on black thinkers, they still largely frame their endeavor in reference to the geo-political boundaries of the U.S. nation-state, thereby ignoring the transnational and diasporic dynamics of black politics. The consequence is that alternative traditions of thought in the Americas—e.g., Caribbean traditions—are cast as irrelevant to questions of racial exclusion in U.S. political thinking. I seek to correct nation-centric perspectives on U.S. political thought and development by demonstrating the utility of the “transnational turn.” Drawing on the framework developed in C.L.R. James’sThe Black Jacobins,I trace how an influential cohort of abolitionists in the antebellum United States looked to the Haitian Revolution as a model for the overthrow of slavery. Engaging the writings and speeches of David Walker, James Theodore Holly, and Frederick Douglass, I then argue that radical abolitionists operated in the same ideological problem-space as Haitian revolutionaries and adopted a specific model of revolution as much indebted to Haitian political thought as Anglo-American models of anti-colonial revolt. By implication, racially egalitarian movements and moments in U.S. political development cannot be adequately understood with exclusive reference to national traditions of thought.
Democratic Peace: Progress and Crisis
Authors: Hobson Christopher
Published: August 2017

When work on the democratic peace first emerged it contributed to the revitalization of liberal thought and represented an important contribution to International Relations (IR). Yet innovation has been replaced by stagnation. Coding and correlation are debatedad infinitum, while little attention is given to growing economic inequality, voter alienation, a decline in traditional parties, rising populism and a wide array of related trends that raise serious doubts about the health of democracies at the center of the zone of peace. Yet if democratic institutions are not functioning as they are meant to, and norms of compromise are disappearing domestically, what hope can there be that these will facilitate cooperative behavior between democracies? Rather than promoting peace, could it be that capitalism in its contemporary neoliberal form is undermining or hollowing out democracy? The static understanding of democracy adopted by this research means that such questions have been largely overlooked. In response, I focus on two major changes impacting established democracies and consider their significance for democratic peace arguments: the decline of democratic institutions and culture, as well as how neoliberalism is reshaping the relationship between democracy and capitalism. In developing this argument, I propose that the template that the democratic peace research program offers for studying the world is emblematic of—and contributing to—a troubling contracting of our political vision. An excessive concern with methodological rigor, combined with a narrow understanding of what qualifies as valid research, has resulted in a body of scholarship that is remarkably sophisticated but has surprisingly little to say about democracy and its place in the world.
Public Sociology in the Age of Social Media
Authors: Healy Kieran
Published: August 2017

I informally examine how the idea of public sociology has been affected by the rise of social media. New social media platforms disintermediate communication, make people more visible, and encourage public life to be measured. They tend to move the discipline from a situation where some people self-consciously do “public sociology” to one where more sociologists unselfconsciously do sociology in public. I discuss the character of such “latently public” work, the opportunities and difficulties it creates for individuals, and its tendency to be associated with academic fields that believe in what they are doing.
Small Wetlands Retain Lion’s Share of Nutrients
Authors: Witman Sarah
Published: August 2017

Still-water ecosystems are key to combating explosive algae growth.
Sediment Cores Reveal Ocean Current’s Past Life
Authors: Witman Sarah
Published: August 2017

East Asia’s Black Current may have rerouted in the past 10,000 years or so.
The Competing Climate Effects of Elevation and Albedo
Authors: Cook Terri
Published: August 2017

Variations in surface reflectivity are as important as surface elevation changes in determining regional climate at nonpolar latitudes, according to a new modeling study.
What Caused the Ongoing Flooding on Lake Ontario?
Authors: Wendel JoAnna
Published: August 2017

The floodwaters have also affected residents downstream along the Saint Lawrence River. Although politicians quickly blamed regulations, scientists say it was a perfect storm of natural factors.
Physiological and ecological implications of an iron- or hydrogen-oxidizing member of the Zetaproteobacteria, Ghiorsea bivora, gen. nov., sp. nov.
Authors: Mori Jiro F , Scott Jarrod J , Hager Kevin W , Moyer Craig L , Küsel Kirsten , Emerson David
Published: August 2017

Chemosynthetic Fe-oxidizing communities are common at diffuse-flow hydrothermal vents throughout the world’s oceans. The foundational members of these communities are the Zetaproteobacteria, a class of Proteobacteria that is primarily associated with ecosystems fueled by ferrous iron, Fe(II). We report here the discovery of two new isolates of Zetaproteobacteria isolated from the Mid-Atlantic Ridge (TAG-1), and the Mariana back-arc (SV-108), that are unique in that they can utilize either Fe(II) or molecular hydrogen (H2) as sole electron donor and oxygen as terminal electron acceptor for growth. Both strains precipitated Fe-oxyhydroxides as amorphous particulates. The cell doubling time on H2 vs Fe(II) for TAG-1 was 14.1 vs 21.8 h, and for SV-108 it was 16.3 vs 20 h, and it appeared both strains could use either H2 or Fe(II) simultaneously. The strains were close relatives, based on genomic analysis, and both possessed genes for the uptake NiFe-hydrogenase required for growth on H2. These two strains belong to Zetaproteobacteria operational taxonomic unit 9 (ZetaOTU9). A meta-analysis of public databases found ZetaOTU9 was only associated with Fe(II)-rich habitats, and not in other environments where known H2-oxidizers exist. These results expand the metabolic repertoire of the Zetaproteobacteria, yet confirm that Fe(II) metabolism is the primary driver of their physiology and ecology.
Erratum: E3 Ubiquitin ligase ZNRF4 negatively regulates NOD2 signalling and induces tolerance to MDP
Authors: Bist Pradeep , Cheong Wan Shoo , Ng Aylwin , Dikshit Neha , Kim Bae Hoon , Pulloor Niyas Kudukkil , Khameneh Hanif Javanmard , Hedl Matija , Shenoy Avinash R. , Balamuralidhar Vanniarajan , Malik Najib Bin Abdul , Hong Michelle , Neutzner Albert , Chin Keh-Chuang , Kobayashi Koichi S. , Bertoletti Antonio , Mortellaro Alessandra , Abraham Clara , MacMicking John D. , Xavier Ramnik J. , Sukumaran Bindu
Published: August 2017

Nature Communications 8 Article number: 15865 (2017); Published: 28 June 2017; Updated: 18 August 2017 The previously published version of this Article contained a line number in the Abstract within the sentence ‘A fraction of RIP2 localizes to the endoplasmic reticulum (ER), where it interacts withZNRF4 under either unstimulated and muramyl dipeptide-stimulated conditions’.
Correction: Corrigendum: Adrenergic-mediated loss of splenic marginal zone B cells contributes to infection susceptibility after stroke
Authors: McCulloch Laura , Smith Craig J. , McColl Barry W.
Published: August 2017

Nature Communications 8 Article number: 15051 (2017); Published: 19 April 2017; Updated: 18 August 2017 The affiliation details for Barry W. McColl are incorrect in this Article. The correct affiliation details for this author are given below: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components
Authors: Ning Bo , Zhao Wei , Qian Chen , Liu Pinghua , Li Qingtian , Li Wenyuan , Wang Rong-Fu
Published: August 2017

Despite much progress in the comprehension of the complex process of somatic cell reprogramming, many questions regarding the molecular mechanism of regulation remain to be answered. At present, the knowledge on the negative regulation of reprogramming process is indeed poor in contrary to the identification of positive regulators. Here we report for the first time that ubiquitin-specific protease 26 negatively regulates somatic cell-reprogramming process by stabilizing chromobox (CBX)-containing proteins CBX4 and CBX6 of polycomb-repressive complex 1 through the removal of K48-linked polyubiquitination. Thus, accumulated CBX4 and CBX6 repress the expression of pluripotency genes, such as Sox2 and Nanog, through PRC1 complexes to ubiquitinate histone H2A at their promoters. In all, our findings have revealed an essential role for ubiquitin-specific protease 26 in cellular reprogramming through polycomb-repressive complex 1.
Reinforcement determines the timing dependence of corticostriatal synaptic plasticity in vivo
Authors: Fisher Simon D. , Robertson Paul B. , Black Melony J. , Redgrave Peter , Sagar Mark A. , Abraham Wickliffe C. , Reynolds John N.J.
Published: August 2017

Plasticity at synapses between the cortex and striatum is considered critical for learning novel actions. However, investigations of spike-timing-dependent plasticity (STDP) at these synapses have been performed largely in brain slice preparations, without consideration of physiological reinforcement signals. This has led to conflicting findings, and hampered the ability to relate neural plasticity to behavior. Using intracellular striatal recordings in intact rats, we show here that pairing presynaptic and postsynaptic activity induces robust Hebbian bidirectional plasticity, dependent on dopamine and adenosine signaling. Such plasticity, however, requires the arrival of a reward-conditioned sensory reinforcement signal within 2 s of the STDP pairing, thus revealing a timing-dependent eligibility trace on which reinforcement operates. These observations are validated with both computational modeling and behavioral testing. Our results indicate that Hebbian corticostriatal plasticity can be induced by classical reinforcement learning mechanisms, and might be central to the acquisition of novel actions.
Ancestor of land plants acquired the DNA-3-methyladenine glycosylase (MAG) gene from bacteria through horizontal gene transfer
Authors: Fang Huimin , Huangfu Liexiang , Chen Rujia , Li Pengcheng , Xu Shuhui , Zhang Enying , Cao Wei , Liu Li , Yao Youli , Liang Guohua , Xu Chenwu , Zhou Yong , Yang Zefeng
Published: August 2017

The origin and evolution of land plants was an important event in the history of life and initiated the establishment of modern terrestrial ecosystems. From water to terrestrial environments, plants needed to overcome the enhanced ultraviolet (UV) radiation and many other DNA-damaging agents. Evolving new genes with the function of DNA repair is critical for the origin and radiation of land plants. In bacteria, the DNA-3-methyladenine glycosylase (MAG) recognizes of a variety of base lesions and initiates the process of the base excision repair for damaged DNA. The homologs of MAG gene are present in all major lineages of streptophytes, and both the phylogenic and sequence similarity analyses revealed that green plant MAG gene originated through an ancient horizontal gene transfer (HGT) event from bacteria. Experimental evidence demonstrated that the expression of the maize ZmMAG gene was induced by UV and zeocin, both of which are known as DNA-damaging agents. Further investigation revealed that Streptophyta MAG genes had undergone positive selection during the initial evolutionary period in the ancestor of land plants. Our findings demonstrated that the ancient HGT of MAG to the ancestor of land plants probably played an important role in preadaptation to DNA-damaging agents in terrestrial environments.
Caesarean Section is associated with reduced perinatal cytokine response, increased risk of bacterial colonization in the airway, and infantile wheezing
Authors: Liao Sui-Ling , Tsai Ming-Han , Yao Tsung-Chieh , Hua Man-Chin , Yeh Kuo-Wei , Chiu Chih-Yung , Su Kuan-Wen , Huang Shih-Yin , Kao Chuan-Chi , Lai Shen-Hao , Huang Jing-Long
Published: August 2017

The relationship between cesarean section (CS) and allergic disorders such as asthma and wheezing has been inconsistent, and the mechanisms for their connection remained largely unknown. We aimed to investigate whether CS is associated with infantile wheeze and to explore the connection between CS and several risk factors known to correlate with allergy development. Mononuclear cells were isolated from cord blood and assessed for cytokine responses by ELISA. Bacteria from nasopharyngeal specimens were identified with traditional culture methods. Infant lung function tests were performed at 6 and 12 months of age. IgE levels and clinical outcomes were assessed at 12 months. The result showed that children delivered by CS were associated with increased risk of wheezing (aHR 1.63; 95% CI: 1.01–2.62) and decreased compliance of the respiratory system at 12 months (p = 0.045). In addition, CS was associated with reduced TLR1–2- triggered TNF-α and IL-6 responses at birth. By12 months of age, children delivered by CS had significantly less airway bacterial clearance. Our findings suggested that CS was associated with decreased pro-inflammatory cytokine response to TLR1–2 stimulation, followed by higher abundance of bacterial colonization in the airway during late infancy, thus increasing the risk of infantile wheezing.
Genetic determinants restricting the reassortment of heterologous NSP2 genes into the simian rotavirus SA11 genome
Authors: Mingo Rebecca , Zhang Shu , Long Courtney P. , LaConte Leslie E. W. , McDonald Sarah M.
Published: August 2017

Rotaviruses (RVs) can evolve through the process of reassortment, whereby the 11 double-stranded RNA genome segments are exchanged among strains during co-infection. However, reassortment is limited in cases where the genes or encoded proteins of co-infecting strains are functionally incompatible. In this study, we employed a helper virus-based reverse genetics system to identify NSP2 gene regions that correlate with restricted reassortment into simian RV strain SA11. We show that SA11 reassortants with NSP2 genes from human RV strains Wa or DS-1 were efficiently rescued and exhibit no detectable replication defects. However, we could not rescue an SA11 reassortant with a human RV strain AU-1 NSP2 gene, which differs from that of SA11 by 186 nucleotides (36 amino acids). To map restriction determinants, we engineered viruses to contain chimeric NSP2 genes in which specific regions of AU-1 sequence were substituted with SA11 sequence. We show that a region spanning AU-1 NSP2 gene nucleotides 784–820 is critical for the observed restriction; yet additional determinants reside in other gene regions. In silico and in vitro analyses were used to predict how the 784–820 region may impact NSP2 gene/protein function, thereby informing an understanding of the reassortment restriction mechanism.
Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
Authors: Hur Joonseok , Kim Kyoohyun , Lee SangYun , Park HyunJoo , Park YongKeun
Published: August 2017

Here, the actions of melittin, the active molecule of apitoxin or bee venom, were investigated on human red blood cells (RBCs) using quantitative phase imaging techniques. High-resolution real-time 3-D refractive index (RI) measurements and dynamic 2-D phase images of individual melittin-bound RBCs enabled in-depth examination of melittin-induced biophysical alterations of the cells. From the measurements, morphological, intracellular, and mechanical alterations of the RBCs were analyzed quantitatively. Furthermore, leakage of haemoglobin (Hb) inside the RBCs at high melittin concentration was also investigated.
Biodiversity conservation gaps in the Brazilian protected areas
Authors: Oliveira Ubirajara , Soares-Filho Britaldo Silveira , Paglia Adriano Pereira , Brescovit Antonio D. , de Carvalho Claudio J. B. , Silva Daniel Paiva , Rezende Daniella T. , Leite Felipe Sá Fortes , Batista João Aguiar Nogueira , Barbosa João Paulo Peixoto Pena , Stehmann João Renato , Ascher John S. , de Vasconcelos Marcelo Ferreira , De Marco Paulo , Löwenberg-Neto Peter , Ferro Viviane Gianluppi , Santos Adalberto J.
Published: August 2017

Although Brazil is a megadiverse country and thus a conservation priority, no study has yet quantified conservation gaps in the Brazilian protected areas (PAs) using extensive empirical data. Here, we evaluate the degree of biodiversity protection and knowledge within all the Brazilian PAs through a gap analysis of vertebrate, arthropod and angiosperm occurrences and phylogenetic data. Our results show that the knowledge on biodiversity in most Brazilian PAs remain scant as 71% of PAs have less than 0.01 species records per km2. Almost 55% of Brazilian species and about 40% of evolutionary lineages are not found in PAs, while most species have less than 30% of their geographic distribution within PAs. Moreover, the current PA network fails to protect the majority of endemic species. Most importantly, these results are similar for all taxonomic groups analysed here. The methods and results of our countrywide assessment are suggested to help design further inventories in order to map and secure the key biodiversity of the Brazilian PAs. In addition, our study illustrates the most common biodiversity knowledge shortfalls in the tropics.
Mast cell activation in the acupoint is important for the electroacupuncture effect against pituitrin-induced bradycardia in rabbits
Authors: Zhu Haining , Wang Xuezhi , Huang Meng , Jing Yi , Zhang Di , Ding Guanghong
Published: August 2017

This research was conducted to verify the structural and functional characteristics of mast cells in the electroacupuncture (EA) effects on bradycardia. First, we examined the mast cell density at PC 6, adjacent acupoint LU 7, and a non-acupoint. We tested the effects of EA at PC 6 on heart rate (HR) and blood pressure (BP) in rabbits with pituitrin-induced bradycardia. We also injected sodium cromolyn (Cro), a mast cell membrane stabilizer, at PC 6 30 min before EA to investigate if it affected the EA effects. The results showed that in both PC 6 and LU 7, the mast cell densities were higher than in the non-acupoint (P < 0.05). EA could induce mast cell degranulation at PC 6, which could be suppressed by sodium cromolyn (P < 0.05). EA improved HR, though the change was relatively small in the initial stage with a significant change at 35 min after modelling (P < 0.05). BP significantly improved at 10 min after the onset of pituitrin-induced bradycardia (P < 0.05). The EA effects on both HR and BP were suppressed by sodium cromolyn (P < 0.05). Therefore, we concluded that mast cells in the acupoint are important for the EA effects against pituitrin-induced bradycardia in rabbits.
Genetics of body fat mass and related traits in a pig population selected for leanness
Authors: Reyer Henry , Varley Patrick F. , Murani Eduard , Ponsuksili Siriluck , Wimmers Klaus
Published: August 2017

Obesity is characterized as the excessive accumulation of body fat and has a complex genetic foundation in humans including monogenic high-risk mutations and polygenic contributions. Domestic pigs represent a valuable model on an obesity-promoting high-caloric diet while constantly evaluated for body characteristics. As such, we investigated the genetics of obesity-related traits, comprising subcutaneous fat thickness, lean mass percentage, and growth rate, in a pig population. We conducted genome-wide association analyses using an integrative approach of single-marker regression models and multi-marker Bayesian analyses. Thus, we identified 30 genomic regions distributed over 14 different chromosomes contributing to the variation in obesity-related traits. In these regions, we validated the association of four candidate genes that are functionally connected to the regulation of appetite, processes of adipogenesis, and extracellular matrix formation. Our findings revealed fundamental genetic factors which deserves closer attention regarding their roles in the etiology of obesity.
A comparative analysis of longitudinal computed tomography and histopathology for evaluating the potential of mesenchymal stem cells in mitigating radiation-induced pulmonary fibrosis
Authors: Perez Jessica R. , Lee Sangkyu , Ybarra Norma , Maria Ola , Serban Monica , Jeyaseelan Krishinima , Wang Li Ming , Seuntjens Jan , Naqa Issam El
Published: August 2017

Radiation-induced pulmonary fibrosis (RIPF) is a debilitating side effect that occurs in up to 30% of thoracic irradiations in breast and lung cancer patients. RIPF remains a major limiting factor to dose escalation and an obstacle to applying more promising new treatments for cancer cure. Limited treatment options are available to mitigate RIPF once it occurs, but recently, mesenchymal stem cells (MSCs) and a drug treatment stimulating endogenous stem cells (GM-CSF) have been investigated for their potential in preventing this disease onset. In a pre-clinical rat model, we contrasted the application of longitudinal computed tomography (CT) imaging and classical histopathology to quantify RIPF and to evaluate the potential of MSCs in mitigating RIPF. Our results on histology demonstrate promises when MSCs are injected endotracheally (but not intravenously). While our CT analysis highlights the potential of GM-CSF treatment. Advantages and limitations of both analytical methods are contrasted in the context of RIPF.
Transcriptome changes in Arabidopsis thaliana infected with Pseudomonas syringae during drought recovery
Authors: Gupta Aarti , Senthil-Kumar Muthappa
Published: August 2017

Field-grown plants experience cycles of drought stress and recovery due to variation in soil moisture status. Physiological, biochemical and transcriptome responses instigated by recovery are expected to be different from drought stress and non-stressed state. Such responses can further aid or antagonize the plant’s interaction with the pathogen. However, at molecular level, not much is known about plant-pathogen interaction during drought recovery. In the present study, we performed a microarray-based global transcriptome profiling and demonstrated the existence of unique transcriptional changes in Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tomato DC3000 at the time of drought recovery (drought recovery pathogen, DRP) when compared to the individual drought (D) or pathogen (P) or drought recovery (DR). Furthermore, the comparison of DRP with D or DR and P transcriptome revealed the presence of a few common genes among three treatments. Notably, a gene encoding proline dehydrogenase (AtProDH1) was found to be commonly up-regulated under drought recovery (DR), DRP and P stresses. We also report an up-regulation of pyrroline-5-carboxylate biosynthesis pathway during recovery. We propose that AtProDH1 influences the defense pathways during DRP. Altogether, this study provides insight into the understanding of defense responses that operate in pathogen-infected plants during drought recovery.
Inflammatory cytokine-induced changes in neural network activity measured by waveform analysis of high-content calcium imaging in murine cortical neurons
Authors: Clarkson Benjamin D. S. , Kahoud Robert J. , McCarthy Christina B. , Howe Charles L.
Published: August 2017

During acute neuroinflammation, increased levels of cytokines within the brain may contribute to synaptic reorganization that results in long-term changes in network hyperexcitability. Indeed, inflammatory cytokines are implicated in synaptic dysfunction in epilepsy and in an array of degenerative and autoimmune diseases of the central nervous system. Current tools for studying the impact of inflammatory factors on neural networks are either insufficiently fast and sensitive or require complicated and costly experimental rigs. Calcium imaging offers a reasonable surrogate for direct measurement of neuronal network activity, but traditional imaging paradigms are confounded by cellular heterogeneity and cannot readily distinguish between glial and neuronal calcium transients. While the establishment of pure neuron cultures is possible, the removal of glial cells ignores physiologically relevant cell-cell interactions that may be critical for circuit level disruptions induced by inflammatory factors. To overcome these issues, we provide techniques and algorithms for image processing and waveform feature extraction using automated analysis of spontaneous and evoked calcium transients in primary murine cortical neuron cultures transduced with an adeno-associated viral vector driving the GCaMP6f reporter behind a synapsin promoter. Using this system, we provide evidence of network perturbations induced by the inflammatory cytokines TNFα, IL1β, and IFNγ.
Frequency, moisture content, and temperature dependent dielectric properties of potato starch related to drying with radio-frequency/microwave energy
Authors: Zhu Zhuozhuo , Guo Wenchuan
Published: August 2017

To develop advanced drying methods using radio-frequency (RF) or microwave (MW) energy, dielectric properties of potato starch were determined using an open-ended coaxial-line probe and network analyzer at frequencies between 20 and 4,500 MHz, moisture contents between 15.1% and 43.1% wet basis (w.b.), and temperatures between 25 and 75 °C. The results showed that both dielectric constant (ε′) and loss factor (ε″) were dependent on frequency, moisture content, and temperature. ε′ decreased with increasing frequency at a given moisture content or temperature. At low moisture contents (≤25.4% w.b.) or low temperatures (≤45 °C), ε″ increased with increasing frequency. However, ε″ changed from decrease to increase with increasing frequency at high moisture contents or temperatures. At low temperatures (25–35 °C), both ε′ and ε″ increased with increasing moisture content. At low moisture contents (15.1–19.5% w.b.), they increased with increasing temperature. The change trends of ε′ and ε″ were different and dependent on temperature and moisture content at their high levels. The penetration depth (d
p
) decreased with increasing frequency. RF treatments may provide potential large-scale industrial drying application for potato starch. This research offers useful information on dielectric properties of potato starch related to drying with electromagnetic energy.
Retinal Vascular Imaging Markers and Incident Chronic Kidney Disease: A Prospective Cohort Study
Authors: Yip Wanfen , Ong Peng Guan , Teo Boon Wee , Cheung Carol Yim-lui , Tai E Shyong , Cheng Ching-Yu , Lamoureux Ecosse , Wong Tien Yin , Sabanayagam Charumathi
Published: August 2017

Retinal microvascular changes indicating microvascular dysfunction have been shown to be associated with chronic kidney disease (CKD) in cross-sectional studies, but findings were mixed in prospective studies. We aimed to evaluate the relationship between retinal microvascular parameters and incident CKD in an Asian population. We examined 1256 Malay adults aged 40–80 years from the Singapore Malay Eye Study, who attended both the baseline (2004–07) and the follow-up (2011–13) examinations and were free of prevalent CKD. We measured quantitative retinal vascular parameters (arteriolar and venular calibre, tortuosity, fractal dimension and branching angle) using a computer-assisted program (Singapore I Vessel Assessment, SIVA) and retinopathy (qualitative parameter) using the modified Airlie house classification system from baseline retinal photographs. Incident CKD was defined as an estimated glomerular filtration rate (eGFR) < 60 mL/min/1.73 m2 + 25% decrease in eGFR during follow-up. Over a median follow-up period of 6 years, 78 (6.21%) developed CKD (70.5% had diabetes). In multivariable models, smaller retinal arterioles (hazards ratio [95% confidence interval] = 1.34 [1.00–1.78]), larger retinal venules (2.35 [1.12–5.94] and presence of retinopathy (2.54 [1.48–4.36]) were associated with incident CKD. Our findings suggest that retinal microvascular abnormalities may reflect subclinical renal microvascular abnormalities involved in the development of CKD.
Functional characterisation of brassinosteroid receptor MtBRI1 in Medicago truncatula
Authors: Cheng Xiaofei , Gou Xiaoping , Yin Hongju , Mysore Kirankumar S. , Li Jia , Wen Jiangqi
Published: August 2017

Brassinosteroids are phytohormones involved in plant development and physiological processes. Brassinosteroids Insensitive 1 (BRI1) is required for BR perception and initiation of subsequent signal transduction in Arabidopsis. In this study, the orthologue of BRI1 in the model legume species Medicago truncatula, MtBRI1, was identified and characterised. Three allelic Tnt1 insertion mutants, mtbri1-1, mtbri1-2, and mtbri1-3, were obtained from the M. truncatula Tnt1 insertion population. mtbri1 mutants displayed characteristic bri1 mutant phenotypes: extreme dwarfness, dark green curled leaves, short primary roots, less lateral roots, and insensitive to exogenous brassinolide (BL). Moreover, mtbri1 mutants show decreased total nodule number and defects in nitrogen fixation. MtBRI1 is able to complement an Arabidopsis BRI1 mutant, bri1-5. Similar to the interaction of BRI1 and BAK1 in Arabidopsis, MtBRI1 interacts with MtSERK1 in vivo. Global gene expression profiling revealed that the expression of BR biosynthesis genes and SAUR genes are significantly altered in mtbri1 mutants. MapMan analysis indicated that genes involved in signaling, hormone, cell wall, and biotic stress responses are over-represented in differentially expressed genes. Taken together, the results indicate that MtBRI1 is the BR receptor in M. truncatula and that BR signaling may play a conserved role in balancing plant growth and defenses.
Investigation of Copper Cysteamine Nanoparticles as a New Type of Radiosensitiers for Colorectal Carcinoma Treatment
Authors: Liu Zhipeng , Xiong Li , Ouyang Guoqing , Ma Lun , Sahi Sunil , Wang Kunpeng , Lin Liangwu , Huang He , Miao Xiongying , Chen Wei , Wen Yu
Published: August 2017

Copper Cysteamine (Cu-Cy) is a new photosensitizer and a novel radiosensitizer that can be activated by light, X-ray and microwave to produce singlet oxygen for cancer treatment. However, the killing mechanism of Cu-Cy nanoparticles on cancer cells is not clear yet and Cu-Cy nanoparticles as novel radiosensitizers have never been tested on colorectal cancers. Here, for the first time, we investigate the treatment efficiency of Cu-Cy nanoparticles on SW620 colorectal cells and elucidate the underlying mechanisms of the effects. The results show that X-ray activated Cu-Cy nanoparticles may kill SW620 cancerscells is in a dose-dependent manner. The JC-1 staining shows the mitochondrial membrane potential is decreased after the treatment. The observations confirm that Cu–Cy nanoparticles may improve X-ray radiotherapy on cancer treatment and X-ray activated Cu-Cy nanoparticles can be efficiently destroy colorectal cancer cells by inducing apoptosis as well as autophagy. As a new type of radiosensitizers and photosensitizers, Cu-Cy nanoparticles have a good potential for colorectal cancer treatment and the discovery of autophagy induced by X-ray irradiated Cu-Cy nanoparticles sheds a good insight to the mechanism of Cu-Cy for cancer treatment as a new radiosensitizers.
Exposure to agricultural pesticide impairs visual lateralization in a larval coral reef fish
Authors: Besson Marc , Gache Camille , Bertucci Frédéric , Brooker Rohan M. , Roux Natacha , Jacob Hugo , Berthe Cécile , Sovrano Valeria Anna , Dixson Danielle L. , Lecchini David
Published: August 2017

Lateralization, i.e. the preferential use of one side of the body, may convey fitness benefits for organisms within rapidly-changing environments, by optimizing separate and parallel processing of different information between the two brain hemispheres. In coral reef-fishes, the movement of larvae from planktonic to reef environments (recruitment) represents a major life-history transition. This transition requires larvae to rapidly identify and respond to sensory cues to select a suitable habitat that facilitates survival and growth. This ‘recruitment’ is critical for population persistence and resilience. In aquarium experiments, larval Acanthurus triostegus preferentially used their right-eye to investigate a variety of visual stimuli. Despite this, when held in in situ cages with predators, those larvae that previously favored their left-eye exhibited higher survival. These results support the “brain’s right-hemisphere” theory, which predicts that the right-eye (i.e. left-hemisphere) is used to categorize stimuli while the left-eye (i.e. right-hemisphere) is used to inspect novel items and initiate rapid behavioral-responses. While these experiments confirm that being highly lateralized is ecologically advantageous, exposure to chlorpyrifos, a pesticide often inadvertently added to coral-reef waters, impaired visual-lateralization. This suggests that chemical pollutants could impair the brain function of larval fishes during a critical life-history transition, potentially impacting recruitment success.
Mutation of the Slt2 ortholog from Cryphonectria parasitica results in abnormal cell wall integrity and sectorization with impaired pathogenicity
Authors: So Kum-Kang , Ko Yo-Han , Chun Jeesun , Kim Jung-Mi , Kim Dae-Hyuk
Published: August 2017

We assessed the biological function of CpSlt2, an ortholog of the cell wall integrity (CWI) MAPK of Saccharomyces cerevisiae, in the chestnut blight fungus Cryphonectria parasitica. The CpSlt2-null mutant exhibited marked changes in colonial growth, near absence of conidiation and aerial hyphae, and abnormal pigmentation. In addition, the CpSlt2-null mutant exhibited CWI-related phenotypic defects including hypersensitivity to cell wall-disturbing agents and other stresses. Electron microscopy revealed the presence of abnormal hyphae such as intrahyphal hyphae. In addition, virulence assays indicated that the CpSlt2 gene plays an important role in fungal pathogenesis. As cultivation of the mutant strains progressed, the majority of the colonies showed sporadic sectorization and mycelia from the sectored area stably maintained the sectored phenotype. Although mycelial growth was partially recovered, the sectored progeny had dramatically impaired virulence, confirming the CpSlt2 gene has a role in pathogenicity. Compared to a previous mutant of the CpBck1 gene, a MAPKKK gene in CWI pathway, the CpSlt2-null mutant showed similar, although not identical, phenotypic changes and most phenotypic changes were less severe than those of the CpBck1-null mutant. These results suggest that the unique sectorization is CWI pathway-specific, though the components in the same CWI pathway have common and specific functions.
Emotional over- and under-eating in early childhood are learned not inherited
Authors: Herle Moritz , Fildes Alison , Steinsbekk Silje , Rijsdijk Fruhling , Llewellyn Clare H.
Published: August 2017

Emotional overeating (EOE) has been associated with increased obesity risk, while emotional undereating (EUE) may be protective. Interestingly, EOE and EUE tend to correlate positively, but it is unclear whether they reflect different aspects of the same underlying trait, or are distinct behaviours with different aetiologies. Data were from 2054 five-year-old children from the Gemini twin birth cohort, including parental ratings of child EOE and EUE using the Child Eating Behaviour Questionnaire. Genetic and environmental influences on variation and covariation in EUE and EOE were established using a bivariate Twin Model. Variation in both behaviours was largely explained by aspects of the environment completely shared by twin pairs (EOE: C = 90%, 95% CI: 89%-92%; EUE: C = 91%, 95% CI: 90%-92%). Genetic influence was low (EOE: A = 7%, 95% CI: 6%-9%; EUE: A = 7%, 95% CI: 6%-9%). EOE and EUE correlated positively (r = 0.43, p < 0.001), and this association was explained by common shared environmental influences (BivC = 45%, 95% CI: 40%-50%). Many of the shared environmental influences underlying EUE and EOE were the same (rC = 0.50, 95% CI: 0.44, 0.55). Childhood EOE and EUE are etiologically distinct. The tendency to eat more or less in response to emotion is learned rather than inherited.
Altered Lipid Metabolism in Recovered SARS Patients Twelve Years after Infection
Authors: Wu Qi , Zhou Lina , Sun Xin , Yan Zhongfang , Hu Chunxiu , Wu Junping , Xu Long , Li Xue , Liu Huiling , Yin Peiyuan , Li Kuan , Zhao Jieyu , Li Yanli , Wang Xiaolin , Li Yu , Zhang Qiuyang , Xu Guowang , Chen Huaiyong
Published: August 2017

Severe acute respiratory syndrome-coronavirus (SARS-CoV) and SARS-like coronavirus are a potential threat to global health. However, reviews of the long-term effects of clinical treatments in SARS patients are lacking. Here a total of 25 recovered SARS patients were recruited 12 years after infection. Clinical questionnaire responses and examination findings indicated that the patients had experienced various diseases, including lung susceptibility to infections, tumors, cardiovascular disorders, and abnormal glucose metabolism. As compared to healthy controls, metabolomic analyses identified significant differences in the serum metabolomes of SARS survivors. The most significant metabolic disruptions were the comprehensive increase of phosphatidylinositol and lysophospha tidylinositol levels in recovered SARS patients, which coincided with the effect of methylprednisolone administration investigated further in the steroid treated non-SARS patients with severe pneumonia. These results suggested that high-dose pulses of methylprednisolone might cause long-term systemic damage associated with serum metabolic alterations. The present study provided information for an improved understanding of coronavirus-associated pathologies, which might permit further optimization of clinical treatments.
A broadband reflective-type half-wave plate employing optical feedbacks
Authors: Zhou Gaochao , Zhu Bo , Zhao Junming , Zhu Guanghao , Jin Biaobing , Feng Yijun , Kang Lin , Xu Weiwei , Chen Jian , Wu Peiheng
Published: August 2017

We propose and demonstrate a type of a broadband half-wave plate that operates in the reflective mode. It consists of a metal grating embedded in a dielectric slab and placed on top of a grounded metal surface. We theoretically show that owing to the optical feedback effect which originates from the wave reflections at the air-dielectric interface, the proposed half-wave plate exhibits a broadened and flattened response when comparing to the case where the feedback effect is absent. Such a prediction is validated using both numerical and experimental works carried out on a half-wave plate designed at 10 GHz. Moreover, our theoretical analysis also reveals that the half-wave plate has an interesting feature of broad angular response. Taking advantage of these features, we experimentally demonstrate that the proposed device can function as a freely tunable linear polarization converter with polarization conversion residues less than −20 dB in a wide frequency band, under the condition that the incident angle is as large as 45 degrees.
Neutron crystallography of photoactive yellow protein reveals unusual protonation state of Arg52 in the crystal
Authors: Yonezawa Kento , Shimizu Nobutaka , Kurihara Kazuo , Yamazaki Yoichi , Kamikubo Hironari , Kataoka Mikio
Published: August 2017

Because of its high pKa, arginine (Arg) is believed to be protonated even in the hydrophobic environment of the protein interior. However, our neutron crystallographic structure of photoactive yellow protein, a light sensor, demonstrated that Arg52 adopts an electrically neutral form. We also showed that the hydrogen bond between the chromophore and Glu46 is a so-called low barrier hydrogen bond (LBHB). Because both the neutral Arg and LBHB are unusual in proteins, these observations remain controversial. To validate our findings, we carried out neutron crystallographic analysis of the E46Q mutant of PYP. The resultant structure revealed that the proportion of the cationic form is higher in E46Q than in WT, although the cationic and neutral forms of Arg52 coexist in E46Q. These observations were confirmed by the occupancy of the deuterium atom bound to the N
η1 atom combined with an alternative conformation of the N(η2)D2 group comprising sp2 hybridisation. Based on these results, we propose that the formation of the LBHB decreases the proton affinity of Arg52, stabilizing the neutral form in the crystal.
Habituation/Fatigue behavior of a synapse memristor based on IGZO–HfO2 thin film
Authors: Jiang Ran , Ma Pengfei , Han Zuyin , Du Xianghao
Published: August 2017

A synaptic memristor based on IGZO and oxygen-deficient HfO2 films has been demonstrated. The memristor exhibits a fatigue response to a monotonic stimulus of voltage pulses, which is analogous to the habituation behavior of biological memory. The occurrence of habituation is nearly simultaneous with the transition from short-term memory to long-term memory. The movement and redistribution of oxygen species with the assistance of polarization in HfO2 layer are responsible for the above results. The observation of habituation behavior proves the potential prospect of memristor on the mimic of biological neuron.
Relationships between serum-induced AhR bioactivity or mitochondrial inhibition and circulating polychlorinated biphenyls (PCBs)
Authors: Park Wook Ha , Kang Sora , Lee Hong Kyu , Salihovic Samira , Bavel Bert van , Lind P. Monica , Pak Youngmi Kim , Lind Lars
Published: August 2017

Metabolic syndrome and mitochondrial dysfunction have been linked to elevated serum levels of persistent organic pollutants (POPs). However, it is not clear which specific POPs contribute to aryl hydrocarbon receptor (AhR)-dependent bioactivity or inhibit mitochondrial function in human subjects. Here, we measured the cumulative bioactivity of AhR ligand mixture (AhR bioactivity) and the effects on mitochondrial function (ATP concentration) in recombinant Hepa1c1c7 cells incubated with raw serum samples obtained from 911 elderly subjects in the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) cohort. Plasma concentrations of 30 POPs and plastic chemicals have previously been determined in the same PIVUS subjects. Linear regression analysis demonstrated that total toxic equivalence (TEQ) values and polychlorinated biphenyls (PCBs) were significantly correlated with AhR bioactivity (positively) and ATP concentration (negatively). Serum AhR bioactivities were positively associated with some PCBs, regardless of their dioxin-like properties, but only dioxin-like PCBs stimulated AhR bioactivity. By contrast, PCBs mediated a reduction in ATP content independently of their dioxin-like properties. This study suggests that AhR bioactivity and ATP concentrations in serum-treated cells may be valuable surrogate biomarkers of POP exposure and could be useful for the estimation of the effects of POPs on human health.
Phospholipase D2 loss results in increased blood pressure via inhibition of the endothelial nitric oxide synthase pathway
Authors: Nelson Rochelle K. , Ya-Ping Jiang , Gadbery John , Abedeen Danya , Sampson Nicole , Lin Richard Z. , Frohman Michael A.
Published: August 2017

The Phospholipase D (PLD) superfamily is linked to neurological disease, cancer, and fertility, and a recent report correlated a potential loss-of-function PLD2 polymorphism with hypotension. Surprisingly, PLD2−/− mice exhibit elevated blood pressure accompanied by associated changes in cardiac performance and molecular markers, but do not have findings consistent with the metabolic syndrome. Instead, expression of endothelial nitric oxide synthase (eNOS), which generates the potent vasodilator nitric oxide (NO), is decreased. An eNOS inhibitor phenocopied PLD2 loss and had no further effect on PLD2−/− mice, confirming the functional relationship. Using a human endothelial cell line, PLD2 loss of function was shown to lower intracellular free cholesterol, causing upregulation of HMG Co-A reductase, the rate-limiting enzyme in cholesterol synthesis. HMG Co-A reductase negatively regulates eNOS, and the PLD2-deficiency phenotype of decreased eNOS expression and activity could be rescued by cholesterol supplementation and HMG Co-A reductase inhibition. Together, these findings identify a novel pathway through which the lipid signaling enzyme PLD2 regulates blood pressure, creating implications for on-going therapeutic development of PLD small molecule inhibitors. Finally, we show that the human PLD2 polymorphism does not trigger eNOS loss, but rather creates another effect, suggesting altered functioning for the allele.