bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Effectiveness of Laser-Assisted Bleaching of the Teeth Discolored due to Regenerative Endodontic Treatment
Authors: Shokouhinejad Noushin , Khoshkhounejad Mehrfam , Hamidzadeh Fatemeh
Published: July 2022

Regenerative endodontic treatments (RETs) as a valuable treatment option to save the immature necrotic teeth, have been reported to be associated with discoloration which is an inevitable unfavorable outcome. The present study aimed to compare three laser-assisted protocols with conventional walking bleaching in terms of bleaching efficacy. Seventy-two human incisor teeth underwent regenerative treatment. A triple antibiotic paste containing minocycline, ciprofloxacin, and metronidazole was used as an intracanal medicament. A human blood clot was applied as a scaffold and capped by a hydraulic calcium silicate-based cement. Ten weeks after the RET procedure, a four-session bleaching course started. Teeth were assigned to four groups: (1) 35% hydrogen peroxide gel, (2) 35% hydrogen peroxide gel + Nd: YAG laser, (3) 35% hydrogen peroxide gel + 980 nm diode laser, and (4) 35% hydrogen peroxide gel + 810 nm diode laser. The color changes (ΔE) were measured before and after bleaching sessions. The data were analyzed using Kruskal–Wallis nonparametric test. The statistical significance level was set at 0.05. Significant discoloration, exceeding the perceptibility threshold (ΔE > 3.7) was observed in all of the samples ten weeks after RET. There was no significant difference between groups in terms of RET-induced discoloration values (

p
>
0.05

). Bleaching either by using 35% hydrogen peroxide or 35% hydrogen peroxide activated by different lasers used in this study resulted in significant tooth whitening (

p
<
0.05

). There was no statistically significant difference among the groups in terms of bleaching efficacy (

p
>
0.05

). Internal bleaching by using 35% hydrogen peroxide is as effective as laser-assisted protocols for correction of crown discoloration in teeth that have undergone RET.
Intervention of Shugan Xiaozhi Decoction on Nonalcoholic Fatty Liver Disease via Mediating Gut‐Liver Axis
Authors: Yang Huili , Feng Lian , Xu Linyi , Jiang Dansheng , Zhai Fenfen , Tong Guangdong , Xing Yufeng
Published: July 2022

Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disease with an increasing incidence rate but few therapies. Shugan Xiaozhi decoction (SX) has demonstrated beneficial effects in treating NAFLD with an unclear mechanism. This study was aimed at investigating the therapeutic mechanism of SX on high‐fat diet‐induced NAFLD rats via the gut‐liver axis. Hepatic steatosis and integrity of intestinal mucosa in NAFLD rats were assessed by histopathological staining. The level of lipid and inflammation were estimated by enzyme‐linked immunosorbent assay. Western Blotting was used to detect apolipoprotein (apo) B48 expression. 16S rRNA analysis was used to measure the changes of gut microbial composition after SX treatment. The expressions of zona occludens 1 protein (ZO‐1), occludin, and secretory immunoglobulin A (sIgA) in the colon were detected by immunostaining to investigate the intestinal barrier function. Our study found that SX reduced hepatic steatosis, the levels of alanine aminotransferase, aspartate aminotransferase, total cholesterol, and triglyceride and apoB48 expression but increased peroxisome proliferator activated receptor α (PPARα) level. Moreover, SX altered the diversity of gut microbiota, upregulating the relative abundance of f_Prevotellaceae, while downregulating f_Bacteroidales_ S24-7, f_Lachnospiraceae, f_Ruminococcaceae, f_Erysipelotrichaceae, and f_Desulfovibrionaceae. By increasing the expression of ZO‐1 and occludin and decreasing the level of proinflammatory factors, including sIgA, lipopolysaccharide, tumor necrosis factor‐α, interleukin‐1β, monocyte chemotactic protein‐1, and transforming growth factor‐β1, SX improved intestinal mucosal integrity and barrier function. Our study illustrated that the gut‐liver axis was a potential way for SX to ameliorate NAFLD, that is, by regulating the expression of PPARα, apoB48, and modulating gut microbiota to protect the intestinal barrier function, and thus alleviate lipid deposition and inflammatory response in the liver.
Epidemiological Characteristics of Pediatric Ocular Trauma in China: A Multicenter Retrospective Hospital-Based Study
Authors: Zhang Yaxin , Feng Kang , Yan Hua
Published: July 2022

Purpose. The objective of the study was to obtain the incidence characteristics of pediatric ocular trauma in mainland China and provide some feasible suggestions of prevention. Methods. The patients with pediatric ocular injuries, who were (≤14) hospitalized between January 1, 2019, and December 31, 2020, were included. Patient demographics (age and sex), injury natural characteristics (causes, site, and types), geographic location, and interval of hospital admission were analyzed. Results. A total of 13525 cases were identified, and 1973 (14.6%) occurred in children (male accounts 70.9%) with a mean (SD) age of 6.5 (3.4) years. Cases among minors in 2020 dropped by 8.3% compared to 2019, while the former vulnerability to eye injuries has been shifted from the outdoors to home (51.4%, 1015/1973). The main causes of injury are flying objects (31.9%), traffic injury (23.5%), and blunt injury (21.8%), which lead to the main types of injury such as penetrating injury (48.3%), rupture of the globe (18.1%), and contusion trauma (13.1%). If the VA after injury is above CF, the VA after discharge is more likely to be above CF (OR 18.3, 11.9–28.1;

p
<
0.001

). Similarly, age (OR 2.0, 1.3–3.1;

p
=
0.001

) and intraocular pressure after injury (OR 0.9, 0.9–1.0;

p
=
0.009

) also affect the intraocular pressure at discharge. Conclusions. In mainland China, the main injury types are penetrating injury and rupture of the globe with home being the most dangerous place. Prevention strategies should focus on parents’ education and protective measures indoors. Visual acuity after injury can be used as a predictor of visual acuity after treatment.
Effects of Different Types of Early Restrictive Fluid Resuscitation on Immune Function and Multiorgan Damage on Hemorrhagic Shock Rat Model in a Hypothermic Environment
Authors: Xu Linlin , Li Lin , Zu Jianyu , Huang Xinyuan , Tian Lin , Sun Yingjie , Diao Yugang
Published: July 2022

Objective. This study was aimed at investigating the effects of different types of fluid restriction fluid resuscitation on the immune dysfunction and organ injury of hemorrhagic shock rats under a hypothermic environment. Methods. SD rats were divided into sham operation group (SHAM), hemorrhagic shock model group (HS), crystal liquid limited resuscitation group (CRLLR), colloidal liquid limited resuscitation group (COLLR), and nonlimited resuscitation group (NLR); rats in each group were placed in a low-temperature environment of 0-5°C for 30 min, and then, a hemorrhagic shock rat model was prepared. Sodium lactate Ringer’s restricted resuscitation solution, hydroxyethyl starch restricted resuscitation solution, and hydroxyethyl starch were used for resuscitation, and hemodynamic examination was performed. The mortality rate, inflammatory factors, oxidative stress factors, and immune function were detected by ELISA. The dysfunction and injury of the intestinal, lung, liver, and kidney were examined by histological methods. Results. Hemorrhagic shock resulted in decreased immune function and activation of inflammation. Unrestricted fluid infusion further activated the inflammatory response. The crystalloid-restricted fluid infusion performed effectively to regulate inflammatory response, promote antioxidative activity, and reduce the immunosuppressive reaction. Rehydration could regulate the coagulation. The hydroxyethyl starch reduced the expression of platelet glycoproteins Ib and IIb/IIIa and blocked the binding of fibrinogen to activated platelets, thereby inhibiting intrinsic coagulation and platelet adhesion and aggregation. Rats in the CRLLR group showed to relieve the injury of the lung, liver, kidney, and intestine from hemorrhagic shock in low-temperature environment. Conclusion. The early application of restrictive crystalloid resuscitation in hemorrhagic shock rats in hypothermic environment showed the best therapy results. Early LR-restrictive fluid replacement promotes the balance of inflammatory response and the recovery of immunosuppressive state, resists oxidative stress, stabilizes the balance of coagulation and fibrinolysis, improves coagulation function, and relieves organ injury.
Research Based on the Application and Exploration of Artificial Intelligence in the Field of Traditional Music
Authors: Yang Yi , Huang Xia
Published: July 2022

Traditional Chinese music has undergone trials and tribulations. To date, traditional music has been gradually improved, preserved, and passed down, both in terms of theoretical works and traditional music varieties. However, the current state of traditional music is still a cause for concern. Whether it is scholars engaged in the study of traditional music, universities, or local government agencies, there are still areas that need to be improved individually. The need to cultivate a future audience for traditional music and to make full use of new media such as the Internet is a priority at this stage. The emergence of any new technology is bound to have some impact on the existing social system, and the emergence of artificial intelligence is no exception. Due to the limitation of technology, only a small number of basic applications have been developed, and it is the future mission of research workers whether they can develop more advanced products for music lovers to experience on the basis of ensuring the basic maturity of these applications. In this paper, we introduce the convolutional deep belief network model based on the restricted Boltzmann machine and apply the convolutional deep belief network algorithm to the music melody recognition. Firstly, it was pretrained by an unsupervised greedy layer-by-layer algorithm. Then, the network parameters were fine-tuned by a supervised network training method, and the recognition ability of the model was improved by adjusting the network parameters. The experimental results show that the recognition effect of the system is more obvious under the condition that the length of music samples is 3 s, and the recognition effect is better when the number of model layers is 2 than that when the number of layers is 1.
Efficacy Investigation of TACE Combined with Lenvatinib and Sintilimab in Intermediate-Stage Hepatocellular Carcinoma
Authors: Zhang Menglong , Lai Weiguo , Zhang Jian , Hu Bijuan , Huang Liyin , Chu Cunkun
Published: July 2022

Objective. To evaluate the effectiveness of transarterial chemoembolization (TACE) combined with lenvatinib and sintilimab in treating patients with midstage hepatocellular carcinoma (HCC). Methods. Sixty-two patients with midstage HCC were enrolled in this study. All of them were firstly treated in our hospital between September 1, 2019, and March 1, 2020. According to different treatment regimens, they were divided into the control group (31 cases, TACE group) and the observation group (31 cases, TACE combined with lenvatinib and sintilimab group). Each patient was followed up for at least 30 months to compare the short-term clinical efficacy and survival rate between the two groups. Results. The objective response rate (ORR) and disease control rate (DCR) of the observation group at 3 months were 77.4% and 93.5%, respectively, which were higher than those of the control group (

P
<
0.05

). The 2-year cumulative overall survival rate of the observation group was 64.5%, which was significantly higher than that of the control group (

P
<
0.05

). The survival curve of the disease-free survival rate in the observation group was higher than that in the control group, and the difference was statistically significant (

X

2

=
4.313

,

P
<
0.05

). Conclusion. TACE combined with lenvatinib and sintilimab in the treatment of Barcelona Clinic Liver Cancer (BCLC) stage B hepatocellular carcinoma can effectively control the tumor progression and prolong the survival time of patients. Those preliminary findings need validation in larger studies, with a prospective design and longer follow-up.
Artificial Intelligence‐Based Data‐Driven Strategy to Accelerate Research, Development, and Clinical Trials of COVID Vaccine
Authors: Sharma Ashwani , Virmani Tarun , Pathak Vipluv , Sharma Anjali , Pathak Kamla , Kumar Girish , Pathak Devender
Published: July 2022

The global COVID‐19 (coronavirus disease 2019) pandemic, which was caused by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), has resulted in a significant loss of human life around the world. The SARS‐CoV‐2 has caused significant problems to medical systems and healthcare facilities due to its unexpected global expansion. Despite all of the efforts, developing effective treatments, diagnostic techniques, and vaccinations for this unique virus is a top priority and takes a long time. However, the foremost step in vaccine development is to identify possible antigens for a vaccine. The traditional method was time taking, but after the breakthrough technology of reverse vaccinology (RV) was introduced in 2000, it drastically lowers the time needed to detect antigens ranging from 5–15 years to 1–2 years. The different RV tools work based on machine learning (ML) and artificial intelligence (AI). Models based on AI and ML have shown promising solutions in accelerating the discovery and optimization of new antivirals or effective vaccine candidates. In the present scenario, AI has been extensively used for drug and vaccine research against SARS‐COV‐2 therapy discovery. This is more useful for the identification of potential existing drugs with inhibitory human coronavirus by using different datasets. The AI tools and computational approaches have led to speedy research and the development of a vaccine to fight against the coronavirus. Therefore, this paper suggests the role of artificial intelligence in the field of clinical trials of vaccines and clinical practices using different tools.
The Mechanism of Rac1 in Regulating HCC Cell Glycolysis Which Provides Underlying Therapeutic Target for HCC Therapy
Authors: Ren Yin-Xiang , Li Xiao-Bin , Liu Wei , Yang Xu-Guang , Liu Xin , Luo Yu
Published: July 2022

Aim. To explore the role of Rac1 on sorafenib resistance in hepatocellular carcinoma. Methods. CCK-8, wound healing assay, Transwell, and cell cycle assay were used to detect the tumor cells development. Cell viability was assessed by MTT. The glycolytic pathway was revealed by cellular metabolism assays. Result. We recovered that Rac1 upregulation was related to HCC patients’ poorer prognosis. Forced expression of Rac1 promoted cell development and sorafenib chemoresistance in HCC cells. Rac1 inhibitor EHop-016 and sorafenib combination markedly prevented cell viability, G2/M phase cycle arrest, and apoptosis than single therapy. Furthermore, combination therapy decreased glycolysis in HCC cells. In vivo, the tumor growth was significantly prevented by combination therapy single therapy. Conclusion. Our research declares that Rac1 inhibition could block sorafenib resistance in HCC by decreasing glycolysis, which would provide an underlying target for HCC therapy.
Nd2O3, Cr2O3, and V2O3 Nanoparticles via Calcination: Synthesis, Characterization, Antimicrobial and Antioxidant Activities
Authors: Al-Fakeh Maged S. , Al-Otaibi Najla F.
Published: July 2022

Nd2O3, Cr2O3, and V2O3 nanoparticles were prepared by calcining the precursor materials that are novel mixed ligand complexes: [Nd(BDC)(ADMPY)(OAc)].H2O, [Cr(BDC)(ADM PY)Cl].H2O, and [V(BDC)(ADMPY)Cl].H2O, where BDC = 1,4-benzenedicarboxylic acid and ADMPY = 2-amino-4,6-dimethyl pyrimidine. The generated compounds were examined through several techniques such as elemental analysis (C.H.N), UV-Vis spectroscopy, thermal analysis (thermogravimetric, differential thermogravimetry, and differential thermal analysis), FT-IR spectra, X-ray diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscope (TEM). The TEM micrographs showed that neodymium oxide nanoparticles assumed agglomerated platelet-like particles, with particle sizes around 30.16 nm, while chromium oxide NPs showed solid block material with compact density and fewer pores with nearly spherical shape and 56.12 nm size. The vanadium oxide NPs were an agglomeration of small spherical nanoparticles of 28.4 nm size. The antimicrobial properties of the samples were assessed using two strains of Gram-positive bacteria, two strains of Gram-negative bacteria, and one strain of yeast. The antimicrobial results demonstrated that a large spectrum of activity characterizes the tested compounds because they are active on Gram-positive and Gram-negative bacteria, especially on Gram-positive strains. The antioxidant activity of prepared compounds was assessed by scavenging free radicals of DPPH. Metal oxide NPs also showed promising results as antioxidants.
Exosomal miR-27b-3p Derived from Hypoxic Cardiac Microvascular Endothelial Cells Alleviates Rat Myocardial Ischemia/Reperfusion Injury through Inhibiting Oxidative Stress-Induced Pyroptosis via Foxo1/GSDMD Signaling
Authors: Zhang Baojian , Sun Chao , Liu Yaozhong , Bai Fan , Tu Tao , Liu Qiming
Published: July 2022

Background. Exosomes derived from cardiac microvascular endothelial cells (CMECs) under hypoxia can mediate cardiac repair functions and alleviate pyroptosis and oxidative stress during ischemia-reperfusion (I/R) injury. This study is aimed at investigating the effect and mechanism of miR-27b-3p underlying hypoxic CMECs-derived exosomes against I/R injury. Methods. CMECs were isolated from the left ventricle of Sprague-Dawley rats, followed by culturing under hypoxic conditions or pretreatment with the miR-27b-3p inhibitor. CMECs-derived exosomes were added into H9C2 cells before hypoxia/reoxygenation (H/R) or injected into the rat heart before I/R injury. An in vivo I/R injury model was established by ligating and releasing the left anterior descending coronary artery. Expression of pyroptosis-related factors was detected using Western blot, and heart infarcted size was determined by the 2,3,5-triphenyl-2H-tetrazpinolium chloride staining method. Dual-Luciferase Reporter assays were performed to analyze the interactions of nmiR-27b-3p-forkhead box O1 (Foxo1) and Gasdermin D- (GSDMD-) Foxo1. Chromatin-immunoprecipitation (ChIP) assays were performed to validate the interactions between forkhead box O1 (Foxo1) and Gasdermin D (GSDMD) and Foxo1-mediated histone acetylation of GSDMD. Results. CMECs were successfully identified from left ventricle of Sprague-Dawley rats. The expressions of Foxo1 and pyroptosis-related proteins (GSDMD, NLPR3, cleaved caspase 1, IL-1β, and IL-18) were upregulated in the rat heart after I/R injury. Treatment of CMEC-derived exosomes, especially that under hypoxic conditions, significantly reduced pyroptosis in the rat heart. miR-27b-3p was significantly upregulated in CMEC-derived exosomes under hypoxic conditions, and miR-27b-3p inhibition in exosomes alleviated its cytoprotection and inhibited oxidative stress in H9C2 cells. Treatment with Foxo1 overexpression plasmids aggravated in vitro H/R and in vivo I/R injury by upregulating pyroptosis-related proteins. Further experiments validated that miR-27b-3p negatively targeted Foxo1, which bound to the promoter region of GSDMD. Conclusions. These results demonstrated a great therapeutic efficacy of miR-27b-3p overexpression in hypoxic CMEC-derived exosomes in preventing the development of myocardial damage post I/R injury through inhibiting Foxo1/GSDMD signaling-induced oxidative stress and pyroptosis.
A De Novo Transcriptome Analysis Identifies Cold‐Responsive Genes in the Seeds of Taxillus chinensis (DC.) Danser
Authors: Fu Jine , Wan Lingyun , Song Lisha , He Lili , Jiang Ni , Long Hairong , Huo Juan , Ji Xiaowen , Hu Fengyun , Wei Shugen , Pan Limei
Published: July 2022

Taxillus chinensis (DC.) Danser, a parasitic plant of the Loranthaceae family, grows by attacking other plants. It has a long history of being used in Chinese medicine to treat multiple chronic diseases. We previously observed that T. chinensis seeds are sensitive to cold. In this study, we performed transcriptome sequencing for T. chinensis seeds treated by cold (0°C) for 0 h, 12 h, 24 h, and 36 h. TRINITY assembled 257,870 transcripts from 223,512 genes. The GC content and N50 were calculated as 42.29% and 1,368, respectively. Then, we identified 42,183 CDSs and 35,268 likely proteins in the assembled transcriptome, which contained 1,622 signal peptides and 6,795 transmembrane domains. Next, we identified 17,217 genes (FPKM > 5) and 2,333 differentially expressed genes (DEGs) in T. chinensis seeds under cold stress. The MAPK pathway, as an early cold response, was significantly enriched by the DEGs in the T. chinensis seeds after 24 h of cold treatment. Known cold‐responsive genes encoding abscisic acid‐associated, aquaporin, C‐repeat binding factor (CBF), cold‐regulated protein, heat shock protein, protein kinase, ribosomal protein, transcription factor (TF), zinc finger protein, and ubiquitin were deregulated in the T. chinensis seeds under cold stress. Notably, the upregulation of CBF gene might be the consequences of the downregulation of MYB and GATA TFs. Additionally, we identified that genes encoding CDC20, YLS9, EXORDIUM, and AUX1 and wound‐responsive family protein might be related to novel mechanisms of T. chinensis seeds exposed to cold. This study is first to report the differential transcriptional induction in T. chinensis seeds under cold stress. It will improve our understanding of parasitic plants in response to cold and provide a valuable resource for future studies.
Anti-Extra Domain B Splice Variant of Fibronectin Antibody–Drug Conjugate Eliminates Tumors with Enhanced Efficacy When Combined with Checkpoint Blockade
Authors: Hooper Andrea T. , Marquette Kimberly , Chang Chao-Pei Betty , Golas Jonathon , Jain Sadhana , Lam My-Hanh , Guffroy Magali , Leal Mauricio , Falahatpisheh Hadi , Mathur Divya , Chen Ting , Kelleher Kerry , Khandke Kiran , Muszynska Elwira , Loganzo Frank , Rosfjord Edward , Lucas Judy , Kan Zhengyan , Subramanyam Chakrapani , O'Donnell Christopher , Neri Dario , Gerber Hans-Peter , May Chad , Sapra Puja
Published: July 2022

Extra domain B splice variant of fibronectin (EDB+FN) is an extracellular matrix protein (ECM) deposited by tumor-associated fibroblasts, and is associated with tumor growth, angiogenesis, and invasion. We hypothesized that EDB+FN is a safe and abundant target for therapeutic intervention with an antibody–drug conjugate (ADC). We describe the generation, pharmacology, mechanism of action, and safety profile of an ADC specific for EDB+FN (EDB-ADC). EDB+FN is broadly expressed in the stroma of pancreatic, non–small cell lung (NSCLC), breast, ovarian, head and neck cancers, whereas restricted in normal tissues. In patient-derived xenograft (PDX), cell-line xenograft (CLX), and mouse syngeneic tumor models, EDB-ADC, conjugated to auristatin Aur0101 through site-specific technology, demonstrated potent antitumor growth inhibition. Increased phospho-histone H3, a pharmacodynamic biomarker of response, was observed in tumor cells distal to the target site of tumor ECM after EDB-ADC treatment. EDB-ADC potentiated infiltration of immune cells, including CD3+ T lymphocytes into the tumor, providing rationale for the combination of EDB-ADC with immune checkpoint therapy. EDB-ADC and anti-PD-L1 combination in a syngeneic breast tumor model led to enhanced antitumor activity with sustained tumor regressions. In nonclinical safety studies in nonhuman primates, EDB-ADC had a well-tolerated safety profile without signs of either on-target toxicity or the off-target effects typically observed with ADCs that are conjugated through conventional conjugation methods. These data highlight the potential for EDB-ADC to specifically target the tumor microenvironment, provide robust therapeutic benefits against multiple tumor types, and enhance activity antitumor in combination with checkpoint blockade.
Common Trajectories of Highly Effective CD19-Specific CAR T Cells Identified by Endogenous T-cell Receptor Lineages
Authors: Wilson Taylor L. , Kim Hyunjin , Chou Ching-Heng , Langfitt Deanna , Mettelman Robert C. , Minervina Anastasia A. , Allen E. Kaitlynn , Métais Jean-Yves , Pogorelyy Mikhail V. , Riberdy Janice M. , Velasquez M. Paulina , Kottapalli Pratibha , Trivedi Sanchit , Olsen Scott R. , Lockey Timothy , Willis Catherine , Meagher Michael M. , Triplett Brandon M. , Talleur Aimee C. , Gottschalk Stephen , Crawford Jeremy Chase , Thomas Paul G.
Published: July 2022

Current chimeric antigen receptor-modified (CAR) T-cell products are evaluated in bulk, without assessing functional heterogeneity. We therefore generated a comprehensive single-cell gene expression and T-cell receptor (TCR) sequencing data set using pre- and postinfusion CD19-CAR T cells from blood and bone marrow samples of pediatric patients with B-cell acute lymphoblastic leukemia. We identified cytotoxic postinfusion cells with identical TCRs to a subset of preinfusion CAR T cells. These effector precursor cells exhibited a unique transcriptional profile compared with other preinfusion cells, corresponding to an unexpected surface phenotype (TIGIT+, CD62Llo, CD27−). Upon stimulation, these cells showed functional superiority and decreased expression of the exhaustion-associated transcription factor TOX. Collectively, these results demonstrate diverse effector potentials within preinfusion CAR T-cell products, which can be exploited for therapeutic applications. Furthermore, we provide an integrative experimental and analytic framework for elucidating the mechanisms underlying effector development in CAR T-cell products.

Significance:

Utilizing clonal trajectories to define transcriptional potential, we find a unique signature of CAR T-cell effector precursors present in preinfusion cell products. Functional assessment of cells with this signature indicated early effector potential and resistance to exhaustion, consistent with postinfusion cellular patterns observed in patients.

This article is highlighted in the In This Issue feature, p. 2007
Age-Adjusted Charlson Comorbidity Index Predicts Survival in Endometrial Cancer Patients
Authors: Di Donato Violante , D’Oria Ottavia , Giannini Andrea , Bogani Giorgio , Fischetti Margherita , Santangelo Giusi , Tomao Federica , Palaia Innocenza , Perniola Giorgia , Muzii Ludovico , Benedetti Panici Pierluigi
Published: July 2022

Objective: Comorbidity scores are increasingly used to reduce potential confounding in oncologic research. This is of paramount importance in endometrial cancer (EC) since it is characterized by quite indolent behavior. Here, we aim to evaluate the impact of various comorbidities and concurrent medications used on survival outcomes, adopting the age-adjusted Charlson comorbidity index (A-CCI). Design: This is an observational study. Charts of 257 EC patients were retrieved. Methods: We retrospectively evaluated data of patients who underwent surgical treatment for EC. A-CCI was calculated by summing the weighted comorbidities and age of each patient. A binomial value was assigned to different comorbidities and different drugs. Oncologic outcomes were evaluated using Cox proportional hazard models adjusted for age. Results: A-CCI ≥3 correlated with more aggressive tumor features (47.6% vs. 26.8%, p = 0.001), higher risk of recurrence (29.7% vs. 11.6%, p = 0.001), death (20.7% vs. 7.1%, p = 0.002), and death due to disease (16.6% vs. 6.3%, p = 0.012). Considering comorbidities and drugs at parsimonious multivariable analysis model: cardiac disease, liver disease, and proton pump inhibitors (PPIs) use were independent predictors of disease-free survival. Cardiac disease, autoimmune disease, and PPIs use were independent predictors of overall survival. Diabetes was the only independent predictor for cause-specific survival. Limitations: The major limitation of the present study is its retrospective nature and the relatively small sample size that limit the possibility to have firm conclusions. Conclusion: Patients with EC are characterized by a high burden of comorbidities. Comorbidities are associated directly with survival outcomes. Further attention is needed to improve the active management of comorbidities soon after EC treatments. Interventional studies are needed to improve patients’ outcomes.
Pregnancy-Related Acute Kidney Injury: Do We Know What to Do?
Authors: Shah Silvi , Verma Prasoon
Published: July 2022

Pregnancy-related AKI is a global health problem and is associated with a higher risk of both maternal and fetal morbidity and mortality. Risk factors for developing AKI during pregnancy include older age, history of preeclampsia, and comorbidities like diabetes. Hyperemesis gravidarum is a common cause of AKI during the first trimester, and conditions such as preeclampsia, acute fatty liver disease of pregnancy, thrombotic thrombocytopenic purpura, hemolytic uremic syndrome, and placental abruption are important causes of AKI later in the pregnancy. Diagnosis of pregnancy-related AKI is challenging due to the lack of standard criteria and overlap of clinical manifestations among different etiologies. Timely diagnosis of pregnancy-related AKI is instrumental. Specific treatment includes steroids and immunosuppressive therapy for glomerulonephritis, prompt delivery for severe preeclampsia and acute fatty liver of pregnancy, plasmapheresis for thrombotic thrombocytopenic purpura, and eculizumab for the atypical hemolytic uremic syndrome. Due to the high complexity, management of pregnancy-related AKI should be performed by a multidisciplinary team consisting of a nephrologist, obstetrician, and neonatologist.
Treatment in a Stroke Unit and Risk Factor Control Reduce Recurrent Stroke Risk
Authors: Shani S.D. , Varma Ravi Prasad , Sarma Sankara P. , Sreelakshmi R.S. , Harikrishnan Ramachandran , Kutty V. Raman , Sylaja P.N.
Published: July 2022

Introduction: Risk factor control is an important predictor of risk of stroke recurrence. The attributable fraction which estimates the excess risk among the exposed stroke survivors has not been studied previously. We studied the attributable fraction for stroke recurrence in consecutive incident cases of recurrent stroke. Methods: A case-control study with incident cases of recurrent stroke and controls matched for age and poststroke period was done. A structured interview was done to collect data on sociodemographic variables, lifestyle, and medication adherence. The risk factors, treatment of index stroke, and outcome were collected. Logistic regression analysis was done to find out the factors associated with stroke recurrence. Attributable fraction and average attributable fraction were calculated. Results: Among the 103 matched pairs, more than 70% were rural residents. Male gender (OR 2.59; 95% CI 1.05–6.42), the presence of depression (OR 8.67; 95% CI 2.80–26.84), memory problem (OR 10.12; 95% CI 2.48–41.34), uncontrolled diabetes (OR 3.19; 95% CI 1.42–7.19), cardioembolic stroke (OR 4.45; 95% CI 1.12–17.62), and index stroke not being treated in a stroke unit (OR 6.60; 95% CI 2.86–15.23) were associated with increased risk of stroke recurrence. The maximum average attributable fraction for stroke recurrence risk was attributed to index stroke not being treated in the stroke unit and uncontrolled diabetes. Conclusion: The index stroke treated in a comprehensive stroke care unit and control of risk factors can reduce recurrent stroke risk among stroke survivors. This population-attributable risk is important in planning secondary stroke prevention strategies.
Nodal Assessment in Endometrial Atypical Hyperplasia
Authors: Capozzi Vito Andrea , Sozzi Giulio , Butera Diana , Chiantera Vito , Ghi Tullio , Berretta Roberto
Published: July 2022

Objective: Atypical endometrial hyperplasia (AH) is the neoplastic precursor more often associated with endometrial cancer (EC). Nowadays, 25–50% of patients subjected to hysterectomy for preoperative AH are diagnosed with EC at the final pathological analysis. Furthermore, there is no consensus on which preoperative AH patients would benefit from sentinel lymph node mapping. This study aimed to evaluate nodal assessment and preoperative cancer risk factors in preoperative AH patients undergoing nodal surgical staging. Methods: Patients undergoing surgical treatment for AH were retrospectively included in the analysis. Patients were divided into two groups (AH and EC groups) based on the final surgical pathology. The ESGO/ESTRO/ESP risk classification was used for EC cases. Design: This was a retrospective study. Results: Of the 207 AH patients treated, 152 cases met the inclusion criteria. Among preoperative AH patients with final EC diagnosis, 39 patients were in the low-risk group (25.7%), 8 in the intermediate-risk group (5.3%), 4 in high-intermediate (2.6%), and 3 patients were allocated in the high-risk group (2.0%). Fifty-four total patients underwent nodal surgical staging. Only one nodal micrometastasis (0.7%) was found at ultrastaging. Multivariate analysis showed abnormal uterine bleeding (AUB) (p = 0.01), hypertension (p < 0.01), and endometrial thickness ≥20 mm (p = 0.02) statistically more represented in patients with EC at final surgical analysis. EC risk was 2.9 (95% CI: 1.29–6.48) in AUB, 2.7 (95% CI: 1.06–6.92) in hypertension, and 3.1 (95% CI: 1.19–7.97) in endometrial thickness ≥20 mm cases. Limitations: The present study has limitations inherent in its retrospective nature. Conclusion: The overall risk of nodal metastases in preoperative AH patients was low. Conversely, 9.9% of the preoperative AH patients belonged to the intermediate or high-risk group for EC at the final histological examination. Preoperative cancer risk factors would identify AH patients for whom nodal staging could be suggested.
Nucleus Composition in Transition-based Dependency
Parsing
Authors: Nivre Joakim , Basirat Ali , Dürlich Luise , Moss Adam
Published: July 2022

Dependency-based approaches to syntactic analysis assume that syntactic structure can be analyzed in terms of binary asymmetric dependency relations holding between elementary syntactic units. Computational models for dependency parsing almost universally assume that an elementary syntactic unit is a word, while the influential theory of Lucien Tesnière instead posits a more abstract notion of nucleus, which may be realized as one or more words. In this article, we investigate the effect of enriching computational parsing models with a concept of nucleus inspired by Tesnière. We begin by reviewing how the concept of nucleus can be defined in the framework of Universal Dependencies, which has become the de facto standard for training and evaluating supervised dependency parsers, and explaining how composition functions can be used to make neural transition-based dependency parsers aware of the nuclei thus defined. We then perform an extensive experimental study, using data from 20 languages to assess the impact of nucleus composition across languages with different typological characteristics, and utilizing a variety of analytical tools including ablation, linear mixed-effects models, diagnostic classifiers, and dimensionality reduction. The analysis reveals that nucleus composition gives small but consistent improvements in parsing accuracy for most languages, and that the improvement mainly concerns the analysis of main predicates, nominal dependents, clausal dependents, and coordination structures. Significant factors explaining the rate of improvement across languages include entropy in coordination structures and frequency of certain function words, in particular determiners. Analysis using dimensionality reduction and diagnostic classifiers suggests that nucleus composition increases the similarity of vectors representing nuclei of the same syntactic type.
A new genus of scutigerid centipedes (Chilopoda) from Western Australia, with new characters for morphological phylogenetics of Scutigeromorpha
Authors: EDGECOMBE GREGORY D. , BARROW LAUREN
Published: July 2022

The scutigerid centipede Allothereua incola Verhoeff, 1925, originally described from the Kimberley region of northwestern Australia, is abundantly represented in the Pilbara region of Western Australia. It forms the basis for a new genus, Pilbarascutigera, resembling Thereuopoda Verhoeff, 1904, and Thereuopodina Verhoeff, 1905, in the form of its female gonopods and in having short, triangular spicula on the tergal plates. Pilbarascutigera unites with Thereuopoda in cladistic analysis of morphological characters based on a pairing of slender, needle-like bristles (Tastborsten sensu Verhoeff) and spines on the posterior tergal plates and the arrangement of sensilla on the metarthron of the female gonopod. Scanning electron microscopic documentation of P. incola informs on numerous aspects of scutigeromorph morphology, including the female subanal plates, tergal prominences, and peristomatic structures.
Taxonomic review of Eurasian Paradelia Ringdahl (Diptera: Anthomyiidae)with descriptions of two new species
Authors: MICHELSEN VERNER
Published: July 2022

Paradelia Ringdahl is a modest-sized, anthomyiid genus found in cool-temperate to arctic situations in northern and elevated parts of Eurasia and North America. A high percentage of the known Eurasian species are more widespread, Holarctic in distribution. The monophyly of the genus is well supported, though it is less easy to place unambiguously in a phylogeny. Most species are rarely collected and there exists no substantial information on the biology of immatures and adults even for the only common species, P. intersecta (Meigen). The Eurasian species are reviewed with illustrateddescriptions and an identification key to males and females. Two new species are described: P. uleforsi sp. nov. from Europe and Mongolia and P. manni sp. nov. from northern Pakistan, making a total of 15 named, recognized species in Eurasia. Known distributions are substantially widened for several species.
Late Neogene Lophophaenidae (Nassellaria, Radiolaria) from the eastern equatorial Pacific
Authors: TRUBOVITZ SARAH , RENAUDIE JOHAN , LAZARUS DAVID , NOBLE PAULA
Published: July 2022

Lophophaenidae is a clade of polycystine radiolarians that was highly abundant and diverse in the Late Neogene–Recent eastern equatorial Pacific (EEP). Despite their importance in fossil plankton assemblages, lophophaenids have been neglected because of their generally small size, complex morphology, and weak taxonomic framework. These challenges have left many lophophaenid concepts poorly defined or lacking formal description. Here we address this with a review of 101 lophophaenid taxa observed in EEP Middle Miocene–Recent marine sediments. We discuss existing lophophaenid genera Amphiplecta Haeckel 1881, Arachnocorallium Haeckel 1887, Arachnocorys Haeckel 1860, Botryopera Haeckel 1887, Ceratocyrtis Bütschli 1882, Lithomelissa Ehrenberg 1847, Lophophaena Ehrenberg 1847, and Peromelissa Haeckel 1881, including full species lists. We describe Pelagomanes n. gen., 23 new species: ​​Amphiplecta kikimorae n. sp., Arachnocorys jorogumoae n. sp., Botryopera amabie n. sp., Botryopera babayagae n. sp., Botryopera bolotniki n. sp., Ceratocyrtis? chimii n. sp., Ceratocyrtis vila n. sp., Lithomelissa alkonost n. sp., Lithomelissa babai n. sp., Lithomelissa dybbuki n. sp., Lithomelissa sirin n. sp., Lophophaena arie n. sp., Lophophaena casperi n. sp., Lophophaena domovoi n. sp., Lophophaena gozui n. sp., Lophophaena ikiryo n. sp., Lophophaena ikota n. sp., Lophophaena kaonashii n. sp., Lophophaena leshii n. sp., Lophophaena rusalkae n. sp., Lophophaena shishigae n. sp., Lophophaena ushionii n. sp., and Pelagomanes ibburi n. sp., and one new subspecies, Arachnocorys pentacantha wanii n. subsp. In addition, we document 35 taxa in open nomenclature, and revise generic assignments of 10 species. The names of 32 previously-described species are upheld, but with clarified synonymies, discussion, and illustrations. This work contributes a practical framework for identifying tropical Late Neogene–Recent lophophaenid taxa, and demonstrates their rich morphological diversity.
How Well Do Multisatellite Products Capture the Space–Time Dynamics of Precipitation? Part II: Building an Error Model through Spectral System Identification
Authors: Guilloteau Clement , Foufoula-Georgiou Efi , Kirstetter Pierre , Tan Jackson , Huffman George J.
Published: July 2022

Satellite precipitation products, as all quantitative estimates, come with some inherent degree of uncertainty. To associate a quantitative value of the uncertainty to each individual estimate, error modeling is necessary. Most of the error models proposed so far compute the uncertainty as a function of precipitation intensity only, and only at one specific spatiotemporal scale. We propose a spectral error model that accounts for the neighboring space–time dynamics of precipitation into the uncertainty quantification. Systematic distortions of the precipitation signal and random errors are characterized distinctively in every frequency–wavenumber band in the Fourier domain, to accurately characterize error across scales. The systematic distortions are represented as a deterministic space–time linear filtering term. The random errors are represented as a nonstationary additive noise. The spectral error model is applied to the IMERG multisatellite precipitation product, and its parameters are estimated empirically through a system identification approach using the GV-MRMS gauge–radar measurements as reference (“truth”) over the eastern United States. The filtering term is found to be essentially low-pass (attenuating the fine-scale variability). While traditional error models attribute most of the error variance to random errors, it is found here that the systematic filtering term explains 48% of the error variance at the native resolution of IMERG. This fact confirms that, at high resolution, filtering effects in satellite precipitation products cannot be ignored, and that the error cannot be represented as a purely random additive or multiplicative term. An important consequence is that precipitation estimates derived from different sources shall not be expected to automatically have statistically independent errors.

Significance Statement

Satellite precipitation products are nowadays widely used for climate and environmental research, water management, risk analysis, and decision support at the local, regional, and global scales. For all these applications, knowledge about the accuracy of the products is critical for their usability. However, products are not systematically provided with a quantitative measure of the uncertainty associated with each individual estimate. Various parametric error models have been proposed for uncertainty quantification, mostly assuming that the uncertainty is only a function of the precipitation intensity at the pixel and time of interest. By projecting satellite precipitation fields and their retrieval errors into the Fourier frequency–wavenumber domain, we show that we can explicitly take into account the neighboring space–time multiscale dynamics of precipitation and compute a scale-dependent uncertainty.
Estimating the Ocean Interior from Satellite Observations in the Kerguelen Area (Southern Ocean): A Combined Investigation Using High-Resolution CTD Data from Animal-Borne Instruments
Authors: Liu Lei , Xue Huijie
Published: July 2022

Observational surface data are utilized to reconstruct the subsurface density and geostrophic velocity fields via the “interior + surface quasigeostrophic” (isQG) method in a subdomain of the Antarctic Circumpolar Current (ACC). The input variables include the satellite-derived sea surface height (SSH), satellite-derived sea surface temperature (SST), satellite-derived or Argo-based sea surface salinity (SSS), and a monthly estimate of the stratification. The density reconstruction is assessed against a newly released high-resolution in situ dataset that is collected by a southern elephant seal. The results show that the observed mesoscale structures are reasonably reconstructed. In the Argo-SSS-based experiment, pattern correlations between the reconstructed and observed density mostly exceed 0.8 in the upper 300 m. Uncertainties in the SSS products notably influence the isQG performance, and the Argo-SSS-based experiment yields better density reconstruction than the satellite-SSS-based one. Through the two-dimensional (2D) omega equation, we further employ the isQG reconstructions to diagnose the upper-ocean vertical velocities (denoted wisQG2D), which are then compared against the seal-data-based 2D diagnosis of wseal. Notable discrepancies are found between wisQG2D and wseal, primarily because the density reconstruction does not capture the seal-observed smaller-scale signals. Within several subtransects, the Argo-SSS-based wisQG2D reasonably reproduce the spatial structures of wseal, but present smaller magnitude. We also apply the isQG reconstructions to the 3D omega equation, and the 3D diagnosis of wisQG3D is very different from wisQG2D, indicating the limitations of the 2D diagnostic equation. With reduced uncertainties in satellite-derived products in the future, we expect the isQG framework to achieve better subsurface estimations.
Revisiting the White Boys From Portland to Ukraine: Anomie and Right-Wing Extremism
Authors: Blazak Randall
Published: July 2022

The 2022 mass shooting in Buffalo, New York that targeted black grocery shoppers followed a now-familiar pattern. A white male, radicalized by online disinformation campaigns, including the narrative that whites are being systematically “replaced” in society, engaged in an act of domestic terrorism to further the cause of white nationalism. This article charts the ways that right-wing extremism has evolved in the first two decades of the twentieth-first century. This recent history begins with the racist skinhead and patriot militia movements that dominated the literature 20 years ago and moves through the rise of the alt-right, lone-wolf attacks, the January 6, 2021, Capitol attack, and finally, situates a globalized movement in a pandemic era of de-globalization that has both direct and indirect connection to the 2022 Russian invasion of Ukraine. The theoretical theme throughout is Emile Durkheim’s concept of anomie and how white males have responded to the rapid pace of social change. Conclusions are drawn from a wide variety of interviews, from Proud Boys in Portland, Oregon to Ukrainian soldiers, stationed on the Ukraine–Poland border.