bluuebunny/crossref_metadata_2025 huggingface dataset:
The Possibility of Regenerating a Pine Stand through Natural Regeneration
Authors: Przybylski Paweł , Konatowska Monika , Jastrzębowski Szymon , Tereba Anna , Mohytych Vasyl , Tyburski Łukasz , Rutkowski Paweł
Published: August 2021

Scots pine (Pinus sylvestris L.) is a widespread species in Eurasia, but its natural range can be significantly altered by a variety of stressors. The ability of pine to regenerate naturally is significantly reduced by its occurrence in unsuitable habitats. The processes of natural selection of pine from select habitats can be followed in stands of national parks where forestry activities are excluded. The possibility of pine regeneration is influenced by the following factors: characteristics of produced seeds, competition, and genetic characteristics. In the present study, selected factors associated with limiting the natural regeneration potential of pine were analysed. The present work generated important information related to the natural regeneration potential of pine in Central and Eastern Europe. The main objective of the analyses was to discuss the possibility of the natural regeneration of pine stands without human intervention. In addition, the genetic diversity of naturally germinating seedlings was analysed. The obtained results confirmed the high reproductive potential of pine despite the advanced age of the studied trees. The obtained seeds produced by old growth Scots pine trees had high viability, while a significant difference was observed in terms of the average number of cones per dominant tree between studied stands. Thus, the number of cones was the main element determining the regeneration potential of the stands. It should be emphasised that the number of cones did not influence the occurrence of natural regeneration. Based on the obtained results, the regeneration potential of pine stands depends mainly on the habitat and the competitive pressure. In addition, a correlation between genetic parameters and the regeneration potential of stands should be established, which may be the beginning of further research on the process discussed in this publication.
Pyrrolizidine Alkaloids in the Food Chain: Is Horizontal Transfer of Natural Products of Relevance?
Authors: Chmit Mohammad Said , Horn Gerd , Dübecke Arne , Beuerle Till
Published: August 2021

Recent studies have raised the question whether there is a potential threat by a horizontal transfer of toxic plant constituents such as pyrrolizidine alkaloids (PAs) between donor-PA-plants and acceptor non-PA-plants. This topic raised concerns about food and feed safety in the recent years. The purpose of the study described here was to investigate and evaluate horizontal transfer of PAs between donor and acceptor-plants by conducting a series of field trials using the PA-plant Lappula squarrosa as model and realistic agricultural conditions. Additionally, the effect of PA-plant residues recycling in the form of composts or press-cakes were investigated. The PA-transfer and the PA-content of soil, plants, and plant waste products was determined in form of a single sum parameter method using high-performance liquid chromatography mass spectroscopy (HPLC-ESI-MS/MS). PA-transfer from PA-donor to acceptor-plants was frequently observed at low rates during the vegetative growing phase especially in cases of close spatial proximity. However, at the time of harvest no PAs were detected in the relevant field products (grains). For all investigated agricultural scenarios, horizontal transfer of PAs is of no concern with regard to food or feed safety.
Potential of Ultraviolet-Visible Spectroscopy for the Differentiation of Spanish Vinegars According to the Geographical Origin and the Prediction of Their Functional Properties
Authors: González-Domínguez Raúl , Sayago Ana , Fernández-Recamales Ángeles
Published: August 2021

High-quality wine vinegars with unique organoleptic characteristics are produced in southern Spain under three Protected Designations of Origin (PDO), namely “Jerez”, “Condado de Huelva” and “Montilla-Moriles”. To guarantee their authenticity and avoid frauds, robust and low-cost analytical methodologies are needed for the quality control and traceability of vinegars. In this study, we propose the use of ultraviolet-visible spectroscopy in combination with multivariate statistical tools to discriminate Spanish wine vinegars according to their geographical origin, as well as to predict their physicochemical and functional properties. Linear discriminant analysis provided a clear clustering of vinegar samples according to the PDO with excellent classification performance (98.6%). Furthermore, partial least squares regression analysis demonstrated that spectral data can serve as accurate predictors of the total phenolic content and antioxidant activity of vinegars. Accordingly, UV-Vis spectroscopy stands out as a suitable analytical tool for simple and rapid authentication and traceability of vinegars.
No Glycation Required: Interference of Casein in AGE Receptor Binding Tests
Authors: Zenker Hannah E. , Teodorowicz Malgorzata , Wichers Harry J. , Hettinga Kasper A.
Published: August 2021

For the determination of the binding of heated cow’s milk whey proteins such as β-lactoglobulin to the receptors expressed on immune cells, inhibition ELISA with the soluble form of the receptor for advanced glycation end products (sRAGE) and scavenger receptor class B (CD36) has been successfully used in the past. However, binding to heated and glycated caseins in this read-out system has not been tested. In this study, inhibition ELISA was applied to measure the binding of cow’s milk casein alone, as well as all milk proteins together, which underwent differential heat treatment, to sRAGE and CD36, and we compared those results to a dot blot read out. Moreover, binding to sRAGE and CD36 of differentially heated milk protein was measured before and after in vitro digestion. Casein showed binding to sRAGE and CD36, independent from the heat treatment, in ELISA, while the dot blot showed only binding to high-temperature-heated milk protein, indicating that the binding is not related to processing but to the physicochemical characteristics of the casein. This binding decreased after passage of casein through the intestinal phase.
In Vitro Bioaccessibility and Antioxidant Activity of Polyphenolic Compounds from Spent Coffee Grounds-Enriched Cookies
Authors: Castaldo Luigi , Lombardi Sonia , Gaspari Anna , Rubino Mario , Izzo Luana , Narváez Alfonso , Ritieni Alberto , Grosso Michela
Published: August 2021

Spent coffee ground (SCG) is a significant by-product generated by the coffee industry. It is considered a great source of bioactive molecules well-recognized for exerting biological properties. This study aimed to implement SCG in a baked foods, such as cookies (SCGc), to increase their bioactive potential. A comprehensive study of the polyphenolic fraction of the SCG and SCGc using a high-resolution mass spectrometry analysis was performed. Moreover, the polyphenol bioaccessibility and change in antioxidant activity during simulated gastrointestinal digestion (GiD) were assessed. Data showed that SCGc provided 780 mg of melanoidins, 16.2 mg of chlorogenic acid (CGA), 6.5 mg of caffeine, and 0.08 mg of phenolic acids per 100 g of sample. Moreover, the 5-caffeoylquinic acid was the most relevant CGA found in SCG (116.4 mg/100 g) and SCGc (8.2 mg/100 g) samples. The antioxidant activity evaluated through three spectrophotometric tests, and the total phenolic compounds of SCGc samples exhibited significantly higher values than the control samples. Furthermore, during simulated GiD, the highest bioaccessibility of SCGc polyphenols was observed after the colonic stage, suggesting their potential advantages for human health. Therefore, SCG with high content in bioactive molecules could represent an innovative ingredient intended to fortify baked food formulations.
Differences in the Levels of the Selected Phytoestrogens and Stable Isotopes in Organic vs. Conventional Hops and Beer
Authors: Golubović Jelena B. , Heath Ester , Košir Iztok Jože , Ogrinc Nives , Potočnik Doris , Strojnik Lidija , Heath David
Published: August 2021

Xanthohumol (XN), isoxanthohumol (IX) and 8-prenylnaringenin (8-PN) are important prenylflavonoids present in hops with potential beneficial properties. In this study, we examined differences in the content of XN, IX and 8-PN in hops and beer produced under organic and conventional production regimes. A An ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) method for analysing XN, IX and 8-PN in hops and beer was developed and validated, with LOQ ranging from 0.5 to 10 ng/mL. Finally, we examined 15N/14N and 12C/13C isotope ratios in the hops and beer using isotope ratio mass spectrometry (IRMS). The results show no statistically significant difference in the content of the selected prenylflavonoids between organic and conventionally produced hops and beer—in the whole sample group, as well as between the matched pairs. Stable isotope analysis indicated that only δ15N values are statistically higher in organically produced hops and beer. However, the differentiation according to the type of production could not be made solely based on the δ15N signature, but it could be used to provide supporting evidence.
Adsorptive Removal of Heavy Metal Ions, Organic Dyes, and Pharmaceuticals by DNA–Chitosan Hydrogels
Authors: Chan Kayee , Morikawa Kohki , Shibata Nobuyuki , Zinchenko Anatoly
Published: August 2021

DNA–chitosan (DNA–CS) hydrogel was prepared by in situ complexation between oppositely charged DNA and chitosan polyelectrolytes via electrostatic cross-linking to study its adsorption characteristics. The DNA–chitosan hydrogel matrix contains (i) cationic (NH3+) and anionic (PO4−) sites for electrostatic binding with ionic species, (ii) -OH and -NH2 groups and heteroaromatic DNA nucleobases for chelation of heavy metal ions, and (iii) DNA double-helix for recognition and binding to small organic molecules of various structures and polarities. DNA–CS hydrogels efficiently bind with Hg2+, Pb2+, Cd2+, and Cu2+ metal cations of significant environmental concern. Adsorption capacities of DNA–CS hydrogels for studied metal ions depend on hydrogel composition and pH of solution and reach ca. 50 mg/g at neutral pHs. Hydrogels with higher DNA contents show better adsorption characteristics and notably higher adsorption capacity to Hg2+ ions. Because of the co-existence of cationic and anionic macromolecules in the DNA–CS hydrogel, it demonstrates an affinity to both anionic (Congo Red) and cationic (Methylene Blue) dyes with moderate adsorption capacities of 12.6 mg/g and 29.0 mg/g, respectively. DNA–CS hydrogel can also be used for adsorptive removal of pharmaceuticals on conditions that their molecules are sufficiently hydrophobic and have ionogenic group(s). Facile preparation and multitarget adsorption characteristics of DNA–CS hydrogel coupled with sustainable and environmentally friendly characteristics render this system promising for environmental cleaning applications.
Using Functional Annotations to Study Pairwise Interactions in Urinary Tract Infection Communities
Authors: Lara Elena G. , van der Windt Isabelle , Molenaar Douwe , de Vos Marjon G. J. , Melkonian Chrats
Published: August 2021

The behaviour of microbial communities depends on environmental factors and on the interactions of the community members. This is also the case for urinary tract infection (UTI) microbial communities. Here, we devise a computational approach that uses indices of complementarity and competition based on metabolic gene annotation to rapidly predict putative interactions between pair of organisms with the aim to explain pairwise growth effects. We apply our method to 66 genomes selected from online databases, which belong to 6 genera representing members of UTI communities. This resulted in a selection of metabolic pathways with high correlation for each pairwise combination between a complementarity index and the experimentally derived growth data. Our results indicated that Enteroccus spp. were most complemented in its metabolism by the other members of the UTI community. This suggests that the growth of Enteroccus spp. can potentially be enhanced by complementary metabolites produced by other community members. We tested a few putative predicted interactions by experimental supplementation of the relevant predicted metabolites. As predicted by our method, folic acid supplementation led to the increase in the population density of UTI Enterococcus isolates. Overall, we believe our method is a rapid initial in silico screening for the prediction of metabolic interactions in microbial communities.
Suggested Absence of Horizontal Transfer of Retrotransposons between Humans and Domestic Mammal Species
Authors: Wanner Nicole , Faulk Christopher
Published: August 2021

Transposable element sequences are usually vertically inherited but have also spread across taxa via horizontal transfer. Previous investigations of ancient horizontal transfer of transposons have compared consensus sequences, but this method resists detection of recent single or low copy number transfer events. The relationship between humans and domesticated animals represents an opportunity for potential horizontal transfer due to the consistent shared proximity and exposure to parasitic insects, which have been identified as plausible transfer vectors. The relatively short period of extended human–animal contact (tens of thousands of years or less) makes horizontal transfer of transposons between them unlikely. However, the availability of high-quality reference genomes allows individual element comparisons to detect low copy number events. Using pairwise all-versus-all megablast searches of the complete suite of retrotransposons of thirteen domestic animals against human, we searched a total of 27,949,823 individual TEs. Based on manual comparisons of stringently filtered BLAST search results for evidence of vertical inheritance, no plausible instances of HTT were identified. These results indicate that significant recent HTT between humans and domesticated animals has not occurred despite the close proximity, either due to the short timescale, inhospitable recipient genomes, a failure of vector activity, or other factors.
Organization of DNA Replication Origin Firing in Xenopus Egg Extracts: The Role of Intra-S Checkpoint
Authors: Ciardo Diletta , Haccard Olivier , Narassimprakash Hemalatha , Arbona Jean-Michel , Hyrien Olivier , Audit Benjamin , Marheineke Kathrin , Goldar Arach
Published: August 2021

During cell division, the duplication of the genome starts at multiple positions called replication origins. Origin firing requires the interaction of rate-limiting factors with potential origins during the S(ynthesis)-phase of the cell cycle. Origins fire as synchronous clusters which is proposed to be regulated by the intra-S checkpoint. By modelling the unchallenged, the checkpoint-inhibited and the checkpoint protein Chk1 over-expressed replication pattern of single DNA molecules from Xenopus sperm chromatin replicated in egg extracts, we demonstrate that the quantitative modelling of data requires: (1) a segmentation of the genome into regions of low and high probability of origin firing; (2) that regions with high probability of origin firing escape intra-S checkpoint regulation and (3) the variability of the rate of DNA synthesis close to replication forks is a necessary ingredient that should be taken in to account in order to describe the dynamic of replication origin firing. This model implies that the observed origin clustering emerges from the apparent synchrony of origin firing in regions with high probability of origin firing and challenge the assumption that the intra-S checkpoint is the main regulator of origin clustering.
Litigation in Anesthesia and Intensive Care Units: An Italian Retrospective Study
Authors: Petrucci Emiliano , Vittori Alessandro , Cascella Marco , Vergallo Alessandro , Fiore Gilberto , Luciani Antonio , Pizzi Barbara , Degan Giulia , Fineschi Vittorio , Marinangeli Franco
Published: August 2021

Anesthesiologists consider professional insurance and its medico-legal problems as a remarkable aspect of their job. “Associazione Anestesisti Rianimatori Ospedalieri Italiani—Emergenza ed Area Critica” (AAROI-EMAC) is the Italian professional association of anesthesiologists and intensivists that works to train its subscribers on safety measures. This is a retrospective observational study on an insurance complaints database for anesthetic accidents that result in injuries to patients. The analyzed period runs from 1 January 2014 to 31 December 2016. A total of 1309 complaints related to 873 insurance claims were analyzed. Criminal complaints comprised 805 (64.4%) of the total, and civil complaints were 445 (35.6%). The iatrogenic damage claimed included: death (58% of the cases); peripheral nerve damage (8%); spinal cord injuries (5%); unspecified injuries (7%); dental damage (4%); infections (3%); needing second surgical procedure (2%); and other injuries (13%). There is a statistical significance between the size of the hospital and the number of the claims: small hospital complaints comprised 40.1% of the cases, while complaints against medium-sized and large hospitals constituted 20.6% of the cases (χ2GL = 8 = 39.87, p = 0.00). In Italy, anesthesiologists and intensivists are often involved in litigation even when they are not directly responsible for iatrogenic injuries, and the most frequent claims in ICU are related post-operative complications.
Healthcare Safety Nets during the COVID-19 Pandemic Based on Double Diamond Model: A Concept Analysis
Authors: Park Bom-Mi , Lee Hyun-Jung
Published: August 2021

The purpose of this study was to analyze the concept of the “healthcare safety net” during the COVID-19 pandemic. Walker and Avant’s process of concept analysis was used in this systematic literature review. The attributes of the concept of a healthcare safety net during the COVID-19 pandemic were found to be: (a) capacity, (b) accessibility, (c) health equality, and (d) education. In consideration of these defining criteria, antecedents to the concept were identified as: (a) the COVID-19 pandemic, (b) health inequalities (internal factors and external factors), and (c) healthcare systems (health insurance, screening, protective equipment, medicine, and medical services). Consequences of the concept were: (a) meeting healthcare needs, (b) quality of life, and (c) a decrease in morbidity and mortality. A healthcare safety net is an important concept during the COVID-19 pandemic. In situations like COVID-19, healthcare safety nets are designed to meet safety needs, improve quality of life, and reduce patient turnover and mortality. Based on the results of this study, the development of standardized tools for measuring a healthcare safety net as well as that of policies and systems for resolving a healthcare safety net in the COVID-19 situation is recommended.
Lung Ultrasound in Pediatrics and Neonatology: An Update
Authors: Ammirabile Angela , Buonsenso Danilo , Di Mauro Antonio
Published: August 2021

The potential role of ultrasound for the diagnosis of pulmonary diseases is a recent field of research, because, traditionally, lungs have been considered unsuitable for ultrasonography for the high presence of air and thoracic cage that prevent a clear evaluation of the organ. The peculiar anatomy of the pediatric chest favors the use of lung ultrasound (LUS) for the diagnosis of respiratory conditions through the interpretation of artefacts generated at the pleural surface, correlating them to disease-specific patterns. Recent studies demonstrate that LUS can be a valid alternative to chest X-rays for the diagnosis of pulmonary diseases, especially in children to avoid excessive exposure to ionizing radiations. This review focuses on the description of normal and abnormal findings during LUS of the most common pediatric pathologies. Current literature demonstrates usefulness of LUS that may become a fundamental tool for the whole spectrum of lung pathologies to guide both diagnostic and therapeutic decisions.
Effect of Physical Activity on Adolescent Obesity Status over Time: A Latent Growth Modeling Approach
Authors: Chung Sophia Jihey
Published: August 2021

This study aims to examine the longitudinal influence of physical activity (PA) on obesity among normal-weight adolescents in South Korea. A total of 1347 data samples from the Korean Children and Youth Panel Survey from 2011 to 2014 were used. Latent growth modeling was applied. PA was assessed by the hours spent on PA during physical education (PE) classes in the last week. Body mass index for age was considered to determine obesity status. PA during PE positively predicted the intercept of obesity status, but it was not associated with the slope of obesity status. Although a longitudinal effect of PA was not identified, PA could influence the initial status of obesity. Because obesity in early years could steadily influence future obesity status, early detection and intervention for adolescent obesity are necessary. Moreover, more studies examining the effect of PA during PE on obesity status among adolescents should be conducted to identify the association between PA during PE and obesity status over time.
A Blockchain and Artificial Intelligence-Based, Patient-Centric Healthcare System for Combating the COVID-19 Pandemic: Opportunities and Applications
Authors: Jabarulla Mohamed Yaseen , Lee Heung-No
Published: August 2021

The world is facing multiple healthcare challenges because of the emergence of the COVID-19 (coronavirus) pandemic. The pandemic has exposed the limitations of handling public healthcare emergencies using existing digital healthcare technologies. Thus, the COVID-19 situation has forced research institutes and countries to rethink healthcare delivery solutions to ensure continuity of services while people stay at home and practice social distancing. Recently, several researchers have focused on disruptive technologies, such as blockchain and artificial intelligence (AI), to improve the digital healthcare workflow during COVID-19. Blockchain could combat pandemics by enabling decentralized healthcare data sharing, protecting users’ privacy, providing data empowerment, and ensuring reliable data management during outbreak tracking. In addition, AI provides intelligent computer-aided solutions by analyzing a patient’s medical images and symptoms caused by coronavirus for efficient treatments, future outbreak prediction, and drug manufacturing. Integrating both blockchain and AI could transform the existing healthcare ecosystem by democratizing and optimizing clinical workflows. In this article, we begin with an overview of digital healthcare services and problems that have arisen during the COVID-19 pandemic. Next, we conceptually propose a decentralized, patient-centric healthcare framework based on blockchain and AI to mitigate COVID-19 challenges. Then, we explore the significant applications of integrated blockchain and AI technologies to augment existing public healthcare strategies for tackling COVID-19. Finally, we highlight the challenges and implications for future research within a patient-centric paradigm.
The Chinese Government’s Response to the Pandemic: Measures, Dynamic Changes, and Main Patterns
Authors: He Yuxi , Li Maorui , Zhong Qixi , Li Qi , Yang Ruishi , Lin Jing , Zhang Xiaojun
Published: August 2021

(1) Background: The governance measures that governments deploy vary substantially across countries and even within countries; there is, however, limited knowledge of the responses of local governments or from different areas in the same country. (2) Methods: By using grounded theory and an automatic text processing method, this study analyses the pandemic governance measures, the pandemic governance pattern, and possible factors across 28 provinces in mainland China based on the text of 28 official provincial government Sina microblogs dating from 20 January to 1 July 2020. (3) Results and discussion: The provincial pandemic governance patterns in China are divided into a pathogen-control pattern, a diagnosis and treatment consolidation pattern, a balanced promotion pattern, a quick-adjustment response pattern, and a recovery-oriented pattern. The pandemic severity, economic development, public health service, and population structure may all have an impact on pandemic governance measures. (4) Conclusions: The conclusions of this study may help us to reconstruct governance systems related to global public health emergencies from the perspective of normalisation, as well as providing important clarification for management and a reference for countries seeking to curb the global spread of a pandemic.
Characteristics of COVID-19-Related Free Telephone Consultations by Public Health Nurses in Japan: A Retrospective Study
Authors: Sumikawa Yuka , Honda Chikako , Yoshioka-Maeda Kyoko , Yamamoto-Mitani Noriko
Published: August 2021

This study aimed to (1) describe the characteristics of community residents who used coronavirus disease 2019 (COVID-19)-related telephone consultation systems by public health nurses (PHNs) and (2) analyze the concerns they had during the first wave of COVID-19 in Japan. Among 1126 telephone consultations, PHNs recorded telephone consultations between 25 March, and 30 April, 2020, in City A, Japan. We analyzed 1017 consultations involving 799 (79%) community residents (resident group) and 218 (21%) organizational representatives (organization group) located in City A. Half of the consultations were made during midmorning, and most of the consultations were regarding COVID-19 symptoms. Among the resident group, visiting a primary care doctor was the most common recommendation by the PHNs; there was no difference in provision of consultation by sex. Health- and welfare-related organizations mainly consulted PHNs about “having COVID-19-related symptoms” and “undergoing PCR testing,” and PHNs’ recommended them to visit a primary care doctor and coordinate PCR testing. The results suggest that public health centers should provide more helpful information on COVID-19 that accurately reflects the concerns of the population.
The Isolation and Identification of Anthocyanin-Related GSTs in Chrysanthemum
Authors: Li Yajing , Liu Xiaofen , Li Fang , Xiang Lili , Chen Kunsong
Published: August 2021

Anthocyanin is the crucial pigment for the coloration of red chrysanthemum flowers, which synthesizes in the cytosol and is transported to the vacuole for stable storage. In general, glutathione S-transferases (GSTs) play a vital role in this transport. To date, there is no functional GST reported in chrysanthemums. Here, a total of 94 CmGSTs were isolated from the chrysanthemum genome, with phylogenetic analysis suggesting that 16 members of them were clustered into the Phi subgroup which was related to anthocyanin transport. Among them, the expression of CmGST1 was positively correlated with anthocyanin accumulation. Protein sequence alignment revealed that CmGST1 included anthocyanin-related GST-specific amino acid residues. Further transient overexpression experiments in tobacco leaves showed that CmGST1 could promote anthocyanin accumulation. In addition, a dual-luciferase assay demonstrated that CmGST1 could be regulated by CmMYB6, CmbHLH2 and CmMYB#7, which was reported to be related to anthocyanin biosynthesis. Taken together, we suggested that CmGST1 played a key role in anthocyanin transport and accumulation in chrysanthemums.
Sociocultural Dimensions of Children’s Physical Activity in Contemporary Pastoralist Maasai Society
Authors: Tian Xiaojie , Kidokoro Tetsuhiro , Mwangi Francis Mundia
Published: August 2021

Children’s physical activity (CPA) in low- and middle-income regions has received increasing attention, but research is still very limited. This study explores the CPA in contemporary pastoralist Maasai society in rural Kenya by considering its sociocultural dimensions. The physical activity of 25 children (15 girls and 10 boys) was documented with mixed methods, including an epidemiological assessment of the CPA and semi-structured interviews with the targeted children regarding their daily activities. These methods were integrated with the ethnographic data on children’s socialization in the same area. Results showed a very high level of moderate-to-vigorous physical activity (MVPA) of these children with significant gender differences especially outside school. Children reported their continued social participation in local gender–age labor divisions outside of school. As their activities outside school strongly contributed to their empirical learning of local knowledge and skills, a high MVPA plays an active role in enhancing the children’s ability to access and manage livestock and different natural resources. Findings from this study first show that the CPA is not merely physical, but also has significant sociocultural meanings in the process of in situ learning of local wisdom. We call more attention to children’s social roles in future investigations of CPA among less examined populations.
Development and Validation of an Instrument to Measure Work-Related Stress among Rescue Workers in Traumatic Mass-Casualty Disasters
Authors: Chen Yu-Long , Tzeng Wen-Chii , Chao En , Chiang Hui-Hsun
Published: August 2021

Rescue workers are a population at high-risk for mental problems as they are exposed to work-related stress from confrontation with traumatic events when responding to a disaster. A reliable measure is needed to assess rescue workers’ work-related stress from their surveillance of a disaster scene to help prevent severe PTSD and depressive symptoms. The purpose of this study was to develop and validate the Work-Related Stress Scale (WRSS) designed to measure stress in rescue workers after responding to traumatic mass-casualty events. An exploratory sequential mixed methods procedure was employed. The qualitative phase of the item generation component involved in-depth interviews of 7 experienced rescue workers from multiple specialties who had taken part in 1 or 2 mass-casualty events: the 2018 Hualien earthquake or the 2016 Tainan earthquake. In the quantitative phase, a modified Delphi approach was used to achieve consensus ratings by the same 7 raters on the items and to assess content validity. Construct validity was determined by confirmatory factor analysis using a broader sample of 293 rescue workers who had taken part in 1 of 2 mass-casualty events: the 2018 Hualien earthquake or the 2021 Hualien train derailment. The final WRSS consists of 16 items total and 4 subscales: Physical Demands, Psychological Response, Environmental Interruption, and Leadership, with aggregated alphas of 0.74–0.88. The WRSS was found to have psychometric integrity as a measure of stress in rescue workers after responding to a disaster.
A Biomechanical Comparison of the Safety-Bar, High-Bar and Low-Bar Squat around the Sticking Region among Recreationally Resistance-Trained Men and Women
Authors: Kristiansen Eirik , Larsen Stian , Haugen Markus E. , Helms Eric , van den Tillaar Roland
Published: August 2021

Barbell placement can affect squat performance around the sticking region. This study compared kinematics, kinetics, and myoelectric activity of the safety-bar squat with the high-bar, and low-bar squat around the sticking region. Six recreationally resistance-trained men (26.3 ± 3.1 years, body mass: 81 ± 7.7 kg) and eight women (22.1 ± 2.2 years, body mass: 65.7 ± 10.5 kg) performed three repetition maximums in all three squat conditions. The participants lifted the least load with the safety bar followed by the high-bar and then the low-bar squat. Greater myoelectric activity of the gluteus maximus was observed during safety-bar squats than high-bar squats. Also, larger knee extension moments were observed for the safety bar compared with low-bar squat. Men had higher myoelectric activity in the safety-bar condition for the gluteus maximus during all regions in comparison with women, and greater knee valgus at the second occurrence of peak barbell velocity. Our findings suggest that the more upright torso inclination during the safety-bar could allow greater gluteus maximus contribution to the hip extensor moment. Moreover, low-bar squats allowed the greatest loads to be lifted, followed by the high-bar and safety-bar squats, possibly due to the larger hip moments and similar knee moments compared to the other squat conditions. Therefore, when the goal is to lift the greatest load possible among recreationally trained men and women, they should first attempt squatting with a low-bar technique, and if the goal is to increase myoelectric activity in the gluteus maximus, a safety-bar squat may be the more effective than the high- bar squat.
Behaviour of Children and Adolescents and the Use of Mobile Phones in Primary Schools in the Czech Republic
Authors: Kopecký Kamil , Fernández-Martín Francisco-Domingo , Szotkowski René , Gómez-García Gerardo , Mikulcová Klára
Published: August 2021

Today’s young people spend most of their time in contact with mobile devices. Their excessive use carries many risks, such as addiction, cyberbullying and social disruption. Based on this, this study analysed the mobile phone use of young Czechs between 7 and 17 years old (n = 27.177) and assessed the differences in their behaviour according to the mobile device use policies of their schools. The results show that the use of mobile phones was linked to the one of the social networks, YouTube and videogames for the most part. Similarly, those young people who had them at school preferred to use them, instead of practicing sports or social activities. On the other hand, in the centres in which the use of mobile phones was prohibited, they felt bored and without activities to do. Therefore, it will be necessary for schools to implement educational policies that encourage activities and areas of social interaction in the school, especially during recess. However, at the same time, it is recommended not to prohibit the use of technological devices in the educational centre, since this fact encourages students to use them secretly and increases their desire to use them. To this end, its use in the classroom is advocated from an educational perspective, thus promoting collaborative learning and increasing student motivation.
A Walk in the Park? Examining the Impact of App-Based Weather Warnings on Affective Reactions and the Search for Information in a Virtual City
Authors: Tomczyk Samuel , Rahn Maxi , Markwart Henriette , Schmidt Silke
Published: August 2021

Background: Warning apps can provide personalized public warnings, but research on their appraisal and impact on compliance is scarce. This study introduces a virtual city framework to examine affective reactions when receiving an app-based warning, and subsequent behavioral intentions. Methods: In an online experiment, 276 participants (M = 41.07, SD = 16.44, 62.0% female) were randomly allocated to one of eight groups (warning vs. no warning, thunderstorm vs. no thunderstorm, video vs. vignette). Participants were guided through a virtual city by a mock-up touristic app (t1). Then, the app issued a warning about an impending thunderstorm (t2), followed by a virtual thunderstorm (t3). The virtual city tour was presented via vignettes or videos. ANCOVAs were used to investigate trajectories of momentary anxiety, hierarchical regressions analyzed the impact of momentary anxiety on information seeking. Results: Participants who received a warning message and were confronted with a thunderstorm showed the highest increase in momentary anxiety, which predicted information seeking intentions. Conclusions: The findings underscore the importance of affective appraisal in processing warning messages. The virtual city framework is able to differentiate the impact of warning versus event in an online context, and thus promising for future warning research in virtual settings.
Deviant Peer Affiliation and Non-Suicidal Self-Injury among Chinese Adolescents: Depression as a Mediator and Sensation Seeking as a Moderator
Authors: Wei Chang , Li Jingjing , Yu Chengfu , Chen Yanhan , Zhen Shuangju , Zhang Wei
Published: August 2021

Non-suicidal self-injury (NSSI) is an emerging health problem among adolescents. Although previous studies have shown that deviant peer affiliation is an important risk factor for this behavior, the reasons for this relationship are unclear. Based on the integrated theoretical model of the development and maintenance of NSSI and the social development model of delinquency prevention, this study tested whether depression mediated the relationship between deviant peer affiliation and NSSI and whether this mediating effect was moderated by sensation seeking. A sample of 854 Chinese adolescents (31.50% male; Mage = 16.35; SD = 1.15) anonymously completed questionnaires on the study variables. Results of regression-based analyses showed that depression mediated the association between deviant peer affiliation and NSSI, and this effect was stronger among adolescents who reported high sensation seeking. The results demonstrate the role of individual differences in the link between affiliation with deviant peers and NSSI, and have implications for preventing and treating this risky behavior.
Two-Photon Laser Ablation and In Vivo Wide-Field Imaging of Inferior Olive Neurons Revealed the Recovery of Olivocerebellar Circuits in Zebrafish
Authors: Hiyoshi Kanae , Saito Kaito , Fukuda Narumi , Matsuzaki Takahisa , Yoshikawa Hiroshi Y. , Tsuda Sachiko
Published: August 2021

The cerebellum, a brain region with a high degree of plasticity, is pivotal in motor control, learning, and cognition. The cerebellar reserve is the capacity of the cerebellum to respond and adapt to various disorders via resilience and reversibility. Although structural and functional recovery has been reported in mammals and has attracted attention regarding treatments for cerebellar dysfunction, such as spinocerebellar degeneration, the regulatory mechanisms of the cerebellar reserve are largely unidentified, particularly at the circuit level. Herein, we established an optical approach using zebrafish, an ideal vertebrate model in optical techniques, neuroscience, and developmental biology. By combining two-photon laser ablation of the inferior olive (IO) and long-term non-invasive imaging of “the whole brain” at a single-cell resolution, we succeeded in visualization of the morphological changes occurring in the IO neuron population and showed at a single-cell level that structural remodeling of the olivocerebellar circuit occurred in a relatively short period. This system, in combination with various functional analyses, represents a novel and powerful approach for uncovering the mechanisms of the cerebellar reserve, and highlights the potential of the zebrafish model to elucidate the organizing principles of neuronal circuits and their homeostasis in health and disease.
Cigarette Prices and Smoking Behavior in Israel: Findings from a National Study of Adults (2002–2017)
Authors: Shuval Kerem , Stoklosa Michal , Nargis Nigar , Drope Jeffrey , Tzafrir Shay , Keinan-Boker Lital , DeFina Laura F. , Qadan Mahmoud
Published: August 2021

Tobacco taxation and price policies are considered the most effective for lowering demand for tobacco products. While this statement is based on research from numerous countries, scant evidence exists on this topic for Israel. Accordingly, we assessed the association between cigarette prices and smoking prevalence and intensity from a national sample of adults in Israel (2002–2017). Data on smoking behavior were derived from the Israeli Knowledge Attitudes and Practices (KAP) survey, a repeated cross-sectional survey. Price information is from the Economist Intelligence Unit (EIU) since it was not collected in the KAP survey. We used the price of a pack of 20 cigarettes for Marlboro and the local brand. These two price variables were the primary independent variables, and we adjusted for inflation. The dependent variables were current smoking (yes/no) and smoking intensity, defined as the number of cigarettes smoked per week. Multivariable analysis was employed using a two-part model while adjusting for covariates. The first step of the model utilized logistic regression with current smoking as the dependent variable. The second step examining smoking intensity as the dependent variable, used OLS regression. Price elasticity was estimated as well. Analysis revealed that a one-unit increase (Israeli currency) in the price of local brand of cigarettes was related to 2.0% (OR = 0.98; 95%CI 0.98, 0.99) lower odds of being a current smoker, adjusting for covariates including household income. Moreover, a one unit increase in the price of the local brand of cigarettes was related to consuming 1.49 (95% CI −1.97, −1.00) fewer weekly cigarettes, controlling for household income and covariates. Similar results were found with the Marlboro cigarette prices. The total price elasticity of cigarette demand, given by the sum of price elasticities of smoking prevalence and intensity, showed that a 10.0% increase in the price is associated with a 4.6–9.2% lower cigarette consumption among Israeli adults. Thus, increasing cigarette prices will likely lead to a reduction in cigarette smoking thereby improving public health in Israel.
Clinical Outcomes and Oral Health-Related Quality of Life after Periodontal Treatment with Community Health Worker Strategy in Patients with Type 2 Diabetes: A Randomized Controlled Study
Authors: Hsu Yuan-Jung , Chen Yi-Hui , Lin Kun-Der , Lee Mei-Yueh , Lee Yu-Li , Yu Chih-Kai , Kabasawa Yuji , Huang Hsiao-Ling
Published: August 2021

Interventions engaging community health workers (CHW) for diabetes management aim to improve diabetes care and self-management behaviors among patients. We evaluated the effects of nonsurgical periodontal treatment (NSPT) with the CHW strategy on oral self-care behaviors, periodontal status and oral health-related quality of life (OHQoL) in patients with type 2 diabetes mellitus (T2DM). The participants were randomly assigned to experimental (EG; n = 35) and control (CG; n = 33) groups. All participants received NSPT, whereas the patients in the EG also received one-on-one 30 min lessons from a CHW over 4 weeks. The EG exhibited greater improvement in the probing pocket depth (β = −0.2, effect size [ES] = 0.61) and clinical attachment level (β = −0.2, ES = 0.59) at 1-month follow-up than the CG did. The ES increased over the 1-, 3- and 6-month follow-ups, indicating an increase in OHQoL (ES = 0.19, 0.60, and 0.62, respectively) in the EG. The patients in the EG were more likely to change their oral self-care behaviors than patients in the CG were. The NSPT with CHW strategy had a positive effect on 1-month periodontal treatment outcomes, long-term OHQoL and oral self-care behaviors in patients with T2DM.
Population-Based Data Reveal Factors Associated with Organised and Non-Organised Colorectal Cancer Screening: An Important Step towards Improving Coverage
Authors: Tran Thuy Ngan , Van Hal Guido , Peeters Marc , Jidkova Svetlana , De Schutter Harlinde , Hoeck Sarah
Published: August 2021

We investigated factors associated with organised and non-organised colorectal cancer screening using faecal occult blood tests, based on data from 308 municipalities in Flanders (6.6 million residents, 57% of Belgium) during 2015–2017. Logistic regression with generalized estimating equations was used to assess the associations between municipal characteristics and organised and non-organised screening coverages. Factors associated negatively with both organised and non-organised screening: percentage of people aged 70–74 in the target population [OR (odds ratios) = 0.98, 95%CI (confidence interval): 0.97–0.99 and OR = 0.98, 95%CI: 0.96–0.999, respectively]; negatively with organised screening: average income [OR = 0.97, 95%CI: 0.96–0.98], percentage of people with a non-Belgian/Dutch nationality [OR = 0.962, 95%CI: 0.957–0.967]; positively with organised screening: percentages of men in the target population [OR = 1.13, 95%CI: 1.11–1.14], jobseekers [OR = 1.12, 95%CI: 1.09–1.15] and people with at least one general practitioner (GP) visit in the last year [OR = 1.04, 95%CI: 1.03–1.05]; positively with non-organised screening: number of patients per GP [OR = 1.021, 95%CI: 1.016–1.026], percentage of people with a global medical dossier handled by a preferred GP [OR = 1.025, 95%CI: 1.018–1.031]. This study helps to identify the hard-to-reach subpopulations in CRC screening, and highlights the important role of GPs in the process of promoting screening among non-participants and encouraging non-organised participants to switch to organised screening.
Designing Ecological Security Patterns Based on the Framework of Ecological Quality and Ecological Sensitivity: A Case Study of Jianghan Plain, China
Authors: Su Xueping , Zhou Yong , Li Qing
Published: August 2021

Researchers and managers of natural resource conservation have increasingly emphasized the importance of maintaining a connected network of important ecological patches to mitigate landscape fragmentation, reduce the decline of biodiversity, and sustain ecological services. This research aimed to guide landscape management and decision-making by developing an evaluation framework to construct ecological security patterns. Taking the Jianghan Plain as the study area, we identified key ecological sources by overlaying the spatial patterns of ecological quality (biodiversity, carbon storage, and water yield) and ecological sensitivity (habitat sensitivity, soil erosion sensitivity, and water sensitivity) using the Integrated Valuation of Environmental Services and Tradeoffs (InVEST) model and the Chinese Soil Loss Equation Function. Ecological corridors were obtained by the least-cost path analysis method and circuit theory. A total of 48 ecological sources (3812.95 km2), primarily consisting of water area, forestland, and cropland, were identified. Ninety-one ecological corridors were derived, with a total length of 2036.28 km. Forty barriers and 40 pinch points with the highest improvement coefficient scores or priority scores were selected. There were 11 priority corridors with very high levels of connectivity improvement potential and conservation priority, occupying 16.15% of the total length of corridors. The overall potential for ecological connectivity is high on the Jianghan Plain. Our framework offers a valuable reference for constructing ecological security patterns and identifying sites for ecological restoration at the regional scale.
The Structure of Social Support in Confidant Networks: Implications for Depression
Authors: Wang Liyuan , Young Lindsay E. , Miller Lynn C.
Published: August 2021

Social support differs for depressed and non-depressed individuals. However, the structural features of social supports, as represented via social networks and how they are related to depression, and its mitigation, are unclear. Here, we examine associations between personal support network structures and self-reports of depression and depression mitigation behaviors. Cross-sectional data were collected from participants (n = 1002 adults) recruited from a research volunteer website. Personal support networks were elicited by asking participants to nominate up to six people (i.e., confidants) that they talk to about interpersonal problems (e.g., unpleasant social encounters) and to indicate who knows whom among their confidants. Results show that the confidant networks of depressed and non-depressed participants did not differ in network size or in constraint—i.e., the degree to which confidants’ ties overlap with the ties of the participant. However, depressed participants’ confidants had significantly fewer average ties with one another (mean degree). Irrespective of depression diagnosis, lower network constraint and size predicted greater engagement in depression mitigation behaviors. That is, having relatively large confidant networks within which one can freely navigate one’s personal information can contribute to improvement in depressive outcomes. Implications are further discussed in the discussion section.