bluuebunny/crossref_metadata_2025 huggingface dataset:
Sosialisasi Mutu Pada Industri Ikan Layur Kering Mawardi di Desa Weru Kecamatan Paciran Kabupaten Lamongan Jawa Timur
Authors: Kandias Khunaifi , Intyas Candra Adi
Published: March 2024

Pengolahan hasil perikanan Kabupaten Lamongan dikelompokkan menjadi dua berdasarkan skala usahanya, yaitu skala usaha perorangan (rumah tangga) dan usaha pengolahan hasil perikanan skala industri. Usaha pengolahan hasil perikanan di Kabupaten Lamongan pada umumnya masih berskala rumah tangga.. Tujuan dari PKM ini adalah untuk mendeskripsikan ; profil UKM Ikan Kering Mawardi, aspek produksi (aspek teknis, proses produksiÂ  dan analisis profitabilitas), teknis sosialisasi mutu,dan sarana pengembangan mutu UKM Ikan Kering Mawardi. Metode yang digunakan adalah deskriptif kualitatif yaitu meliputi wawancara,observasi dan dokumentasi dengan cara peneliti turun langsung ke lapang. Teknik sampling yang digunakan yaitu purposive sampling dengan narasumber yang sudah ditentukan yaitu Bapak Mawardi. Hasil dari penelitian ini bahwa adanya sosialisasi UKM Ikan Kering Mawardi. bertujuan untuk memberikan pandangan mengenai manajemen pengembangan usaha ditinjau dari aspek produksi dan aspek pemasaran. Sosialisasi memberikan perubahan terhadap UKM Mawardi yaitu pelaku usaha khususnya UKM Mawardi merasa sangat terbantu mengenai pengadaan alat yang dihibahkan oleh program sosialisasi, Pelaku usaha mempunyai wawasan lebih luas mengenai pengendalian mutu, cara pemasaran khususnya media online, dan cara pengemasan produk yang baik dan menarik, serta wawasan mengenai kandungan gizi yang ada di dalam produknya, Meningkatkan harga jual dengan kemasan yang lebih modern dan menarik dan tentu saja higienis. Sementara untuk pengembangan usaha atau peningkatan pendapatan maupun produksi belum bisa dijalankan dikarenakan masih proses untuk memperluas pasar agar produksi tersebut dapat berkembang
Aspek Teknis (Kontruksi) Tambak terhadap Produktivitas Budidaya Udang Vanname (Litopenaeus vannamei) secara Intensif di PT. Andulang Shrimp Farm, Sumenep, Jawa Timur
Authors: Rizky Putri Nurhanida , Cahyanurani Annisa' Bias , Fahruddin Faisal
Published: March 2024

PT. Andulang Shrimp Farm merupakan salah satu perusahaan yang aktif dalam memproduksi dan pengembangan budidaya udang vaname yang telah menerapkan sistem teknologi intensif. Daya dukung tambak sangat dipengaruhi oleh kesesuaian aspek teknis, terutama dari segi konstruksi, mengingat penerapan sistem budidaya intensif memunculkan permasalahan berupa penurunan daya dukung tambak bagi kehidupan udang yang dibudidayakan. Oleh karena itu, diperlukan adanya analisis kesesuaian aspek teknis (konstruksi) guna mendukung aktivitas budidaya berkelanjutan serta meningkatkan produktivitas. Tujuan dari penelitian ini adalah untuk mengkaji kesesuaian lahan pada PT. Andulang Shrimp Farm dalam menerapkan tambak intensif serta pengaruhnya terhadap produktivitas udang. Hasil skoring yang diperoleh menunjukkan persentase kesesuaian konstuksi tambak sebesar 88%, sehingga dapat disimpulkan bahwa konstruksi tambak di PT. Andulang Shrimp Farm termasuk dalam kategori sangat sesuai untuk tambak intensif. Masing â€“ masing petak pada penelitian ini memiliki produktivitas yang tinggi. Petak A1 dengan luas 2.500m2 memiliki produktivitas 2.12kg/m2 dengan ABW 20,53 gr, SR 87,22% dan FCR 1,16. Sedangkan petak D5 dengan luas 1.116m2 memiliki produktivitas 1.58 kg/m2 dengan ABW 11,73 gr, SR 91,44% dan FCR 1,29.
Analisis Pengembangan Kawasan Hutan Mangrove Di Wilayah Pesisir Pantai Kabupaten Karawang
Authors: Fikri Muhamad Rom Ali , Sam'un Mohamad
Published: March 2024

Luas wilayah kawasan hutan mangrove di Kabupaten Karawang mengalami penurunan. Hal ini menjadi ancaman bagi masyarakat yang tinggal disekitarnya. Hutan mangrove mempunyai banyak fungsi dan manfaat yang penting dalam kehidupan secara biologis, ekologi, fisik dan sosial ekonomi. Kesuksesan pengelolaan mangrove juga ditentukan pada implementasi kebijakan stakeholder diwilayah pesisir. Desa Sukakerta salah satu dari daerah yang mempunya ekosistem mangrove di pesisir pantai Kabupaten Karawang. Masyarakat setempat juga berupaya untuk melakukan pngelolaan guna melestarikan ekosistem mangrove disana. Sehingga kondisi dan factor-faktor yang mendukung dalam pengelolaan ekosistem mangrove perlu sekali dianalasis untuk pengembangan kedepan. Penelitian bertujuan untuk menganalisis pengembangan hutan mangarove sebagai kawasan konservasi dan eduwisata serta strategi pengelolaannya. Metode yang dikgunakan adalah wawancara dengan instansi setempat dan warga lokal di Desa Sukakerta, Cilamaya Wetan, Karawang. Analisa data menggunakan metode SWOT dan kemudian dideskriptifkan. Penelitian memperoleh hasil bahwa pengelolaan hutan mangrove sebagai wilayah konservasi ditunjukkan dengan adanya kegiatan reboisasi dan dibentuknya KUB kreasi alam bahari yang fokus dalam pengelolaan dan pelestarian mangrove. Sedangkan pengelolaan sebagai kawasan eduwisata ditunjukkan dengan dibukanya area untuk sarana edukasi dan penelitian bagi seluruh kalangan masyarakat. Hasil analisis SWOT menunjukkan pengelolaan hutan mangrove di Desa Sukakerta, Kecamatan Cilamaya Wetan, Kabupaten Karawang sudah baik dengan nilai (0,12; 0,84) dengan menunjukkan titik kordinat pada kuadran I yaitu agresif sehingga harus terus dikembangkan. KUB kreasi alam bahari adalah salah satu organisasi yang fokus dengan pengelolaan mangrove di Sukakerta sehingga dapat dijadikan sarana penggerak masyarakat untuk turut serta dalam proses pengelolaan. perlu dibuat perencanaan pembangunan/pengembangan dan pengelolaan kawasan mangrove secara komprehensif dan terintegrasi dengan melibatkan berbagai lintas sektor dari unsur pemerintah, swasta dan masyarakat. Kemudian disusul dengan membuat program kerja dan usulan pembangunan kawasan mangrove serta melakukan jejaring kinerja (network system) pembangunanÂ  antara pemerintah, swasta dan masyarakat.
Kerusakan Pesisir Akibat Sedimentasi dan Abrasi di Pantai Karawang
Authors: Haryani Eny Budi Sri
Published: March 2024

Wilayah pesisir Kabupaten Karawang sangat dipengaruhi oleh gelombang laut Pantai Utara Jawa, Indonesia. Beberapa desa telah rusak wilayah pesisirnya sebagai akibat sedimentasi dan abrasi. Kondisi ini harus diatasi dan oleh karena itu harus diidentifikasi kerusakan yang terjadi dan bagaimana cara mengatasinya. Tujuan dari penelitian ini adalah: (1) mengidentifikasi wilayah pesisir Karawang yang mengalami sedimentasi dan abrasi; (2) mengidentifikasi penyebab terjadinya sedimentasi dan abrasi, serta merumuskan solusi penanganannya. Pengumpulan data terdiri dari data primer dan data sekunder, dengan melakukan survey dan pengukuran langsung meliputi data topografi dan kondisi fisik di sekitar pantai, melihat kondisi abrasi dan sedimentasi melalui citra satelit dari google earth, serta melakukan wawancara dengan beberapa warga setempat untuk konfirmasi data dan informasi. Lokasi survey terdiri dari 6 (enam) titik yaitu daerah yang terkena sedimentasi dan abrasi serta mengalami kerusakan garis pantai, yaitu sepanjang Â± 75 km di 6 (enam) kecamatan dengan penelitian dilakukan selama bulan Juli-September 2019. Hasil penelitian ini, bahwa sedimentasi dan abrasi telah merusak kondisi fisik pesisir dan kehidupan sosial di Karawang, dengan lokasi yang rusak akibat sedimentasi adalah di 4 desa pesisir yaitu Muara Cilamaya Lama, Sukajaya, Pusakajaya Utara, dan Tambaksari. Sedangkan lokasi yang rusak akibat abrasi adalah di 2 desa pesisir yaitu desa Sedari dan Tanjungpakis. Kerusakan tersebut terjadi akibat gempuran ombak dari laut ke daratan, yang menyebabkan kerusakan dan perubahan garis pantai menjorok ke daratan karena sedimentasi, terputusnya jalan transportasi, merusak fasilitas sosial, mengancam keselamatan nelayan selama melaut, mengganggu kegiatan budidaya ikan, dan merusak kelestarian ekosistem pesisir. Solusi untuk penanganannya antara lain dengan membangun bangunan pantai, baik dengan pendekatan struktur keras maupun lunak.
Antibiotic residue analysis of tetracycline and chloramphenicol in banana shrimp (Fenneropenaeus merguiensis) from a traditional shrimp farming in Brondong, Lamongan
Authors: Andriyono Sapto , Fatmawati Alma Ika , Hidayati Nuning Vita , Fitrani Mirna , Rahardja Boedi Setya
Published: March 2024

Banana shrimp is one of the potential export shrimp cultivated in Brondong, Lamongan. Antibiotic residues, including pollutants, can come from cultivation pond areas and environmental pollution. The antibiotics in shrimp are tetracycline groups (oxytetracycline, chlortetracycline, and tetracycline) and chloramphenicol.Â  This study aims to determine the presence of antibiotic residues in banana shrimp and whether the quality of the shrimp has met health safety standards based on antibiotic residue analysis using estimated daily intake (EDI) and hazard quotient (HQ) calculations. Examination of antibiotic residues is carried out at the UPT. Quality Testing and Development of Marine and Fisheries Products in Surabaya. This study used an observation method by taking shrimp samples, measuring pond water quality parameters, and testing the content of antibiotic residues in the laboratory. The examination results showed that residues of the tetracycline and chloramphenicol groups were not detected in banana shrimp. Calculations of EDI and HQ cannot be performed because antibiotic residues are not detected. The HQ value is below one, which indicates a low-risk level category. Banana shrimp from a farming pond in Brondong, Lamongan is safe for human consumption.
POLA PERTUMBUHAN IKAN PETEK (Leiognathus equulus) YANG DIDARATKAN DI PELABUHAN IKAN MUARA ANGKE, JAKARTA
Authors: Yunita Lauura Hermala , Gelis Ester Restiana Endang , Wulanda Yoppie , Heltria Septy , Magwa Rizky Janatul , Ramdhani Farhan
Published: March 2024

Ikan petek (Leiognathus equulus) merupakan ikan hasil tangkapan sampingan oleh nelayan di Pelabuhan Muara Angke, Jakarta , ikan ini merupakan salah satu aset terpentingÂ pada ekosistem perairan. Penelitian ini bertujuan untuk menganalisis hubungan panjang bobot ikan petek yang didaratkan di Pelabuhan Muara Angke Jakarta. Penelitian berlangsung pada bulan April Tahun 2021 dilakukan di Pelabuhan Muara Angke Jakarta. Persamaan hubungan untuk panjang bobot adalah W = aLb. sampel ikan yang didapat yaitu berjumlah 30 ekor dengan ukuran antara 125 â€“ 164 mm dan berat berkisar antara 33 â€“ 67 gr. Ukuran mendominasi pada ukuran 133 â€“ 140 mm yaitu sebanyak 11 ekor sementara pada ukuran 157 â€“ 164 mm hanya terdapat 1 ekor. Hasil analisis menunjukan bahwa nilai b yaitu 2.7412 hal ini menunjukkan bahwa pola pertumbuhan allometrik negatif dimana pertumbuhan panjang ikan lebih cepat daripada pertumbuhan beratnya dan memiliki nilai r yaitu 1 artinya pertumbuhan panjang sangat mempengaruhi pertumbuhan berat pada ikan.Kata Kunci : Allometrik Negatif, Leiognathus equulus, Muara Angke, Panjang-BobotÂ
Return on assets, return on equity, earnings per share, dividend yield, and book-to-market ratio’s effects on stock return
Authors: Atmariani Annisa Aulia Rahma , Agustia Dian
Published: March 2024

This research intends to investigate the ability of Return on assets (ROA), return on equity (ROE), earnings per share (EPS), dividend yield (DY), and book-to-market ratio (BMR) on stock returns. Stock investment is one strategy for investors to channel their finances while boosting wealth due to the high return on equities earned. In this study, company information is represented by financial ratios, which play a significant role in guiding investors in identifying which companies have the potential to give the highest number of returns. With 164 total observations, this research used 56 companies that were part of the LQ45 index on the Indonesian Stock Exchange (IDX) in the 2019–2022 timeframe as a sample study with the aim to examine the effects of ROA, ROE, EPS, dividend yield, and book-to-market ratio on stock return. Using STATA 14.0 software, double linear regression analysis is used in the hypothesis test. The results of this research, as proven by the regression analysis, are as follows: (1) the book-to-market ratio negatively affects stock return; and (2) ROA, ROE, EPS, and dividend yield have no bearing on stock return. This study emphasizes the value of diversifying investment strategies and avoiding a narrow range of financial ratios when choosing which stocks to buy. Investors should evaluate general business conditions and take a wider range of issues into account.
Faktor-faktor yang mempengaruhi kualitas perkawinan pada keluarga suami istri bekerja di Kota Bogor
Authors: Fiscarini Santy , Herawati Tin , Sumarwan Ujang
Published: March 2024

Kualitas perkawinan mencerminkan hubungan harmonis suami istri yang menjadi faktor pencegahan terjadinya perceraian. Tujuan penelitian menganalisis faktor-faktor yang memengaruhi kualitas perkawinan keluarga dengan suami-istri bekerja. Desain penelitian yaitu cross sectional study. Lokasi penelitian di Kota Bogor, Jawa Barat. Tempat penelitian di instansi pemerintahan dan badan usaha milik daerah (BUMD). Populasi penelitian ini adalah suami pada keluarga suami-istri bekerja di instansi pemerintahan dan BUMD Kota Bogor. Jumlah responden sebanyak 200 orang suami (60 orang suami bekerja PNS dan 60 orang suami bekerja pegawai BUMD). Data diperoleh dengan wawancara langsung menggunakan kuesioner terstruktur. Data diolah dan dianalisis dengan SPSS for windows dan SEM-PLS (Structural Equation Modeling - Partial Least Square). Hasil penelitian menunjukkan bahwa kualitas perkawinan pada keluarga suami istri bekerja dipengaruhi oleh interaksi suami istri. Hasil uji SEM juga menunjukkan terdapat pengaruh antar variabel lain pada penelitian ini relasi gender berpengaruh positif terhadap gaya pengasuhan ayah dan interaksi suami istri. Interaksi suami istri berpengaruh positif terhadap gaya pengasuhan ayah. Implikasi penelitian ini adalah pentingnya interaksi suami istri untuk meningkatkan kualitas perkawinan pada keluarga suami istri bekerja.
The perversion of local novel morality and its implementation of learning faces a demographic bonus
Authors: Minto Deri Wan , Putriani Ananda , Wahyuni Sri , Francisco Eleonor
Published: March 2024

The background is based on the phenomenon of deviating aspects of morality which often color Minangkabau novels. As a result, there are writers who criticize especially social problems that occur in local communities. The purpose of this research is to describe aspects of deviating aspects of morality in local novels, especially Ismet Fanany's Bulan Susut by Ismet Fanany and their implementation towards learning in schools in the face of the demographic bonus era. Qualitative and analytical descriptive methods form the basis of this research type and method. Based on the findings (1) Aspects of conscience with a total of 9 data findings with a percentage of 21.96%. (2) Aspects of freedom and responsibility with a total of 11 data findings with a percentage of 26.83%. (3) Aspects of rights and obligations with a total of 8 data findings with a percentage of 19.51%. (4) Aspects of values and norms with a total of 13 data findings with a percentage of 31.70%. Implications in this research are interpreted not to be taught research that is obtained openly which is direct in nature, but this research will utilize positive values as an effort to form good character for learning challenges in the era of demographic bonuses.
Identitas politikus selebritas Ridwan Kamil dalam akun Instagram @ridwankamil
Authors: Purwaramdhona Ananda Bintang , Adji Muhamad , Prabasmoro Tisna
Published: March 2024

Instagram menjadi ruang publik baru di dunia politik yang digunakan untuk memberikan pengaruh dan menarik simpati publik oleh seorang politikus, salah satunya Ridwan Kamil. Artikel ini bertujuan menjelaskan proses membangun dan mempertahankan identitas politikus selebritas Ridwan Kamil dalam akun Instagram @ridwankamil sebagai cara melakukan proyek hegemoni. Metode campuran digunakan dalam artikel ini melalui pendekatan humaniora digital berbasis korpus, kerangka teori analisis wacana kritis, hegemoni, politikus selebritas, dan presentasi diri. Hasil penelitian menunjukkan dua temuan. Pertama, Ridwan Kamil membangun identitas politikus selebritas melalui akun Instagram melalui citra visual untuk menunjukkan ciri fisik dirinya. Kedua, Ridwan Kamil kerap menggunakan kata “Bandung” dan “Jawa Barat” sebagai titik nodal yang mengacu pada wilayah kekuasaan politik yang dimilikinya, lalu menggunakan kata “saya” dan “kita” serta tiga teknik presentasi diri, yakni self-branding, life streaming, dan micro-celebrity untuk mempertahankan identitas politikus selebritas. Artikel ini berargumen bahwa identitas politikus selebritas merupakan proyek hegemoni karena rantai kesetaraan dan ilusi kedekatan yang diciptakan politikus selebritas mengaburkan relasi kuasa, tetapi di sisi lain menunjukkan dirinya sebagai penguasa.
A linguodidactic model of discourse analysis
Authors: Tarnaeva Larisa Petrovna
Published: March 2024

The study aims to develop a scientifically substantiated, theoretically validated, and practically tested linguodidactic model of discourse analysis. The paper analyzes the evolution of the discourse direction in foreign language teaching, marked by stages such as focusing on the formation of the discourse component of communicative competence, developing a discourse-oriented approach to foreign language teaching, viewing discourse as an independent object of foreign language education and creating models of professionally oriented foreign language education based on discourse analysis. The main directions in the study of discourse were analyzed, with a focus on concepts that formed the methodological basis for developing the linguodidactic model of discourse analysis, specifically linguistic and constructionist approaches to discourse analysis. The scientific novelty of the study lies in proposing the linguodidactic model of discourse analysis aimed at professionally oriented foreign language education, based on the theoretical understanding of the fundamental principles of discourse theory. The work uncovered relevant concepts in linguodidactics related to discourse analysis, providing a basis for extrapolating them to issues in foreign language education. The linguodidactic significance of the sociolinguistic approach to discourse analysis was identified in terms of directing students’ attention to the nuances of verbal interactions among representatives of different organizations and public institutions. The importance of critical discourse analysis and discursive psychology in linguodidactics was also noted, with discourse being viewed as a form of social practice. The linguodidactic model of discourse analysis developed in the study includes a system of interconnected methodological techniques, facilitating a phased transition from analyzing and interpreting meanings encapsulated in verbal and nonverbal components of professionally oriented foreign language discourse to the actual production of discursive utterances.
Todo a punto. Todo en orden
Authors: Santos de Unamuno Carmen
Published: March 2024

Las cosas, a veces, no son lo que parecen y el maltrato a la mujer queda escondido detrás de la puerta de la casa de la pareja. Ni siquiera una doctora bien formada y con interés por sus pacientes se da cuenta siempre de lo que no se cuenta en la consulta. Cuando llega la enfermedad, cambian muchas cosas en el seno de la familia, también en su relación con el sistema sanitario. Los cuidados en casa son necesarios, también la compasión y la entrega. Pero esa pareja tiene una historia cuyo peso no se hará notar hasta el final. El sufrimiento del enfermo y del cuidador son abordados por la doctora que se da de bruces con una realidad ignorada. La atención a las necesidades físicas del enfermo puede ser exquisita por parte de la cuidadora, pero si no hay amor, por muy limpias que estén las úlceras, la soledad y el miedo harán que sea muy difícil que la muerte llegue con la serenidad deseada.
nueva y vieja alianza. Reflexiones acerca de la Medicina Narrativa
Authors: Ricci Ricardo Teodoro
Published: March 2024

El presente artículo tiene como tema central la Medicina Narrativa. Intenta expresar sus características más relevantes y busca encontrar parentescos cercanos o lejanos con otras disciplinas y otras corrientes tanto científicas como filosóficas. De ese modo encontramos que, la definición inicial de medicina narrativa aparentemente carece de profundidad y peso específico, muestra por el contrario una sólida fundamentación teórica y empírica, así como una sabia apertura a nuevos aportes y reflexiones.
Acercamiento al emprendimiento informal en Tamaulipas
Authors: Mellado Ibarra César Iván , Sánchez Tovar Yesenia
Published: March 2024

El presente estudio tuvo por objetivo caracterizar el emprendimiento informal en Tamaulipas identificando las motivaciones y efectos de la actividad económica informal en las mujeres emprendedoras.  El estudio cualitativo se realizó a través de cuatro entrevistas a profundidad a mujeres dueñas de negocios de reciente creación sin estar registrados formalmente. Los hallazgos permiten identificar que el principal motivo de emprender informalmente es la falta de oportunidades laborales o trabajos con bajos salarios. Se encontró que el hecho de no estar constituidos formalmente es debido a la falta de conocimiento de las ventajas que la operación en un marco regulatorio genera.
Conductas Externalizantes: Tratamiento Cognitivo-Conductual en Escenarios Escolares
Authors: Mendoza González Brenda , Villasana Hernández Karen Nelly
Published: March 2024

El objetivo fue evaluar la eficacia de un programa de intervención cognitivo conductual para disminuir comportamientos externalizantes en ambientes escolares. Participaron 99 alumnos, 57 niñas y 42 niños de cuarto a sexto grado de primaria. Es una investigación cuantitativa, con diseño experimenta A-B, A es el pretratamiento y B es el programa de intervención cognitivo-conductual, manipulándose la variable: conductas externalizantes. El análisis de resultados consistió en: a) Inspección visual de traslapamiento de conductas externalizantes; b) inspección visual del cambio de tendencia en los datos (fases A-B). Se concluye que el programa de intervención fue efectivo reduciéndose las conductas externalizantes evaluadas, por lo que se ofrece una alternativa de intervención breve para la disminución de las conductas externalizantes en el aula.
Gobierno corporativo y cambio climático: política, innovación, estrategias y legitimidad
Authors: Rodriguez Jasso Alan Francisco , García Flores Francisco , Briseño García Arturo
Published: March 2024

Se busca conocer las prácticas de gobernanza abordadas en la literatura para enfrentar el cambio climático mediante la identificación de variables, métodos, regiones y fuentes de información que faciliten análisis novedosos para los investigadores. Al respecto, se realiza una revisión sistemática de 45 estudios entre 1980 y 2019 publicados en Web of Science. Los resultados sugieren que la política, innovación, operaciones y la legitimidad son variables comunes para abordar el cambio climático. Los hallazgos muestran que mínimos cuadrados ordinarios y los datos de panel son métodos comunes en la literatura. Economías emergentes como India, Brasil, Indonesia, Irán, Corea del Sur y México ofrecen un contexto interesante para explorar. En este sentido, la crisis climática es una de las principales amenazas para la sociedad moderna.
Development and validation of an analytical procedure for the determination of residual protein A in active substances of therapeutic monoclonal antibodies
Authors: Nozdrina E. V. , Mazalev D. A. , Rogozina D. R. , Zhivoderov S. P. , Lyagoskin I. V. , Shukurov R. R.
Published: March 2024

SCIENTIFIC RELEVANCE. An important quality-control issue for therapeutic monoclonal antibodies (mAbs) is the determination of residual protein A leaching from the carrier during the purification of mAbs by affinity chromatography. However, unrelated compounds (immunoglobulins) present in the sample can complicate the immunoenzymatic detection of protein A (matrix effect), potentially leading to false-negative test results. To increase the efficiency of enzyme-linked immunosorbent assay (ELISA), it is necessary to develop a sample preparation step that can irreversibly break the bond in the protein A–mAb complex.

AIM. This study aimed to develop and validate an analytical procedure for the determination of residual protein A in active substances of therapeutic mAbs by ELISA with a test kit comprising in-house reagents.

MATERIALS AND METHODS. Recombinant protein A was used as an antigen. Polyclonal antibodies to protein A were produced by immunising chickens, selecting immunised eggs, and isolating antibodies from these eggs. Protein A-specific antibodies were purified by affinity chromatography. Residual protein A was determined using sandwich ELISA with preliminary sample preparation. The analytical procedure was validated in accordance with the requirements of the State Pharmacopoeia of the Russian Federation (Validation of Analytical Procedures, OFS.1.1.0012).

RESULTS. The authors obtained, isolated, and purified chicken IgY antibodies to recombinant protein A. The authors selected sample preparation conditions for the determination of residual protein A by ELISA and optimum compositions of buffer solutions, including the composition of a denaturing buffer to disrupt the protein A–mAb complex. The developed analytical procedure was validated. According to the measurements of protein A, the accuracy of the analytical procedure ranged within 83–108% of the nominal value, the interlaboratory precision ranged within 96–116%, and the repeatability was up to 13%. The lower limit of quantitation was 10 ng/mL with a minimum required dilution of 1:10. The analytical range extended from 10 to 40 ng/mL. The analytical procedure showed results comparable to those obtained with a similar test kit from an international manufacturer.

CONCLUSIONS. The developed analytical procedure for the determination of residual protein A by ELISA with a test kit comprising in-house reagents can minimise the matrix effect in therapeutic mAbs. This analytical procedure will alleviate import substitution and reduce quality control costs for Russian immunobiologicals.
Quantitative mass spectrometry with ¹⁸O labelling as an alternative approach for determining protease activity: an example of trypsin
Authors: Konstantinov M. A. , Zhdanov D. D. , Toropygin I. Yu.
Published: March 2024

SCIENTIFIC RELEVANCE. In the quality control of proteolytic enzyme components of medicinal products, the activity of proteases is determined by spectrophotometry, which involves mea­suring the amidase or esterase activity using a synthetic substrate and the proteolytic activity using the Anson method. These methods require special substrates and have low sensitivity; their specificity may be insufficient, which may lead to serious errors. Quantitative mass spectrometry is an alternative approach to protease activity assays, which involves adding an isotope-labelled peptide to hydrolysates of the test enzyme. This approach allows determining the activity of proteases, notably, by the hydrolysis of specific peptide bonds, while simulta­neously confirming the identity and specificity of the test sample. Quantitative mass spectrometry has high sensitivity and does not require special substrates.

AIM. This study aimed to investigate the possibility of enzymatic activity assay and enzyme identification by quantitative mass spectrometry with ¹⁸O labelling through an example of trypsin with casein.

MATERIALS AND METHODS. The study used trypsin, casein, and H₂¹⁸O (Izotop, Russia). Peptide separation was performed using an Agilent 1100 HPLC system; mass spectra were obtained using a Bruker Ultraflex II MALDI-TOF/TOF mass spectrometer. Quantitative mass spectrometry was performed using a standard peptide, which was obtained from casein by tryptic digestion and HPLC purification. For ¹⁸O labelling, the authors dried the peptide and incubated it in H₂¹⁸О water. The quantitative analysis of the product was carried out using MALDI-TOF mass spectrometry. The authors used quantitative mass spectrometry with ¹⁸O labelling to determine enzymatic activity and calculate the Michaelis constant (KM).

RESULTS. Following the tryptic digestion of casein, the authors identified the fragments corre­sponding to casein chains. The authors produced the isotope-labelled standard peptide and calculated its concentration using mass spectrometry. The authors determined the rate of casein digestion by trypsin and calculated the KM for trypsin, which was 13.65±0.60 μM. The standard deviation for repeated measurements showed that the mass-spectrometric method had a lower error of measurement than the spectrophotometric method. The sensitivity threshold for the mass-spectrometric method was 0.50±0.08 μM.

CONCLUSIONS. The results obtained with trypsin confirm the possibility of enzymatic activity determination by the proposed method of quantitative mass spectrometry with ¹⁸O labelling. According to the sensitivity evaluation results, this method can be used for the simultaneous determination of enzyme activity, identity, and specificity. The proposed mass spectrometry approach is universal, it does not require expensive materials and reagents, and it can be easily adapted to determine the activity of virtually any protease.
Bandwidth and information capacity of a resource: space, frequency, time
Authors:   ITMO University, 197101, Russia, St. Petersburg, Kronverksky ave., 49, lit. A., Grishentsev A.Yu.
Published: March 2024

Studies of the bandwidth of the resource: space, frequency, time during spatial multiplexing in information transmission, storage and processing systems have been carried out. Bandwidth estimation is relevant in the development of communication systems with spatial multiplexing, as well as in problems of information theory. Limitations in spatial multiplexing are investigated. Theorems for estimating the amount of information passing through a given linear and flat area of space at certain values of the time interval and frequency band are formulated and proved. A theorem for estimating the amount of information passing through a rectangular parallelepiped due to three beams of electromagnetic radiation through three mutually perpendicular faces of a rectangular parallelepiped is formulated and proved. As an example, the amount of information contained in one cubic meter of space filled with electromagnetic radiation of a certain type is estimated. Thus, for frequencies in the range from 3 •108 Hz to 6•1019 Hz, one cubic meter of space contains 3,207•1034 bits of information, which is equivalent to the amount of information contained due to quantum states in a substance weighing 1,444 g at 20 °C. Practical significance: the research results can be useful for the development of information theory and the practice of developing communication systems with spatial multiplexing.
ADAPTING REGIONAL TELEVISION CONTENT TO THE FORMAT OF SOCIAL NETWORKS (ON THE EXAMPLE OF THE VITA TV CHANNEL)
Authors:   Vinnytsia State Pedagogical University named after Mykhailo Kotsyubynskyi, Odobetska Iryna
Published: March 2024

The article examines the peculiarities of the regional media and the specifics of creating the content of the TV channel’s social networks. The prerequisites for the need to distribute TV content in modern forms of the virtual environment are provided. Ukraine is actively implementing digital technologies in television, which leads to the improvement of the quality and diversity of local media, as a result of which regional television becomes more flexible, interactive and adaptive to changes in consumer preferences and technological capabilities. The growing popularity of social media is being integrated into regional television strategies for audience engagement, engagement and rapid response to events. Regional TV channels increasingly use social networks to interact with viewers, publish news and ensure rapid dissemination of information. The process of adaptation of television content of the information and television agency «VITA» to social networks has been studied. Key words: social networks, content, television, VITA TV channel, text, users, mass media.
STRUCTURE OF MEDIAENVIRONMENT THROUGH THE PRISM OF LEXICAL INNOVATIONS
Authors:   Odessа Polytechnic National University, Balandina Nadiya
Published: March 2024

Using theoretical and empirical methods, this paper proves that the modern mediaenvironment is a complex configuration made by the material and the virtual components and is reflected in the language in various ways. Innovative lexis with the component media and its systematization has become the key to understanding the mediareality, in particular, detecting the constituent elements of the structure of the mediatized environment. In total, 455 lexemes chosen from the Ukrainian dictionaries, academic publications, and the results provided by Google search engine have been analyzed. The systematization of the lexical units have been done according to the principle of the lexical and semantic field, and as the result macro-, midi-, and mini-fields have been allocated with the subsequent ranging of the lexis into the core, the centre, and the periphery. Within the macro-field MEDIAENVIRONMENT, the midi-fields have been located – TECHNICAL, TECHNOLOGICAL, and SOCIO-COMMUNICATIVE. The conceptual core of the macro-field MEDIAENVIRONMENT has been shown to form lexemes signifying space specified from the point of view of temporality, structuredness, contextuality, and evaluation. TECHNICAL midi-field is represented by the names of media equipment; TECHNOLOGICAL midi-field – by the names of methods, processes, and ways of using media equipment. The structural and semantic framework of the SOCIO-COMMUNICATIVE concentre is represented by mini-fields: WHO – FOR WHAT PURPOSE – WHAT – CHANNEL – TO WHOM – EFFECTS. The senders of information are shown as social institutions, groups, and individuals. The nominations of the addressee reflect the transformation of the passive information consumer to the user. The informational object has been specified according to its form, meaning, and the virtual «shell». The wide array of names for communication channels shows the link between the social field with the technical and technological ones, with their relative autonomy. The effects of consuming the information are verbalized by a smaller number of lexemes, largely of behavioral and evaluative nature. To sum up, the specified groups of neolexemes with the component media create a qualitative-quantitative hierarchical structure, the most numerous parts of which serve as the indicators of the most media-determined spheres of socioenvironment. Key words: mediaenvironment, structure, verbalization, neolexemes with the component media, lexical-semantical field.
INTERACTIVE STYLES: PERSPECTIVES OF EMERGENCE, ESTABLISHMENT AND DEVELOPMENT
Authors:   Ivan Franko National University of Lviv, Bilovska Natalia
Published: March 2024

Stylistics can be considered as a field of study that crosses text theory, linguistics, and journalism. Although different schools create different approaches to stylistics, each stylistic approach will include in its paradigm some basic factors, such as the reader and the author. This article shows how these factors interact with each other and, ultimately, create the basis for the emergence of a new field in Ukrainian journalism – interactive stylistics. The study is devoted to interactive stylistics, which is considering as a field based on the text’s own pragmatic potential in the context of modern humanities methodology. This discipline acquires a new function: to observe and interpretively explain, firstly, the meaning of interactions between agents (author and recipient) in communication, mediated by the media text, and, secondly, the effect that this interaction brings. At the center of interactive stylistics is the author (journalist), who through the text discusses the content of interactions in relation to his own interests, as well as cultural, social and historical contracts with the reader. The meaning of the expressions used and the general meaning of such interactions arises in the context of communication events, based on the perception of the subjects of communication with the surrounding reality through the assimilation and adequate interpretation of new information. In modern Ukrainian science of communication, the study of interactive stylistics acquires significant potential. It profiles itself in the context of interdisciplinarity and aims to explore interactivity, interpretability, as well as intertextuality (in specific media texts or interdiscourse dialogue). Interactive stylistics is able to meet the needs of communicators as a useful source of instructions on how communication subjects interact and has a chance to achieve success both at the domestic and international scientific level. Due to its scientific perspective of applying the above-mentioned methodology, it is harmoniously integrated among the tendentious linguistic and broader social science and humanitarian disciplines in Ukrainian scientific research or in the wider international context. Keywords: interactive stylistics; stylistics; reverse communication; style; interactivity; media text.
FUNCTIONAL PARAMETERS OF ANTHROPONYM AS ONE OF THE VARIETIES OF FACTUAL MATERIAL IN THE MEDIA TEXT
Authors:   Ivan Franko National University of Lviv, Datsyshyn Chrystyna
Published: March 2024

The main objective of the study is to reveal the functional parameters of anthroponyms in the media texts. Methods of investigation: the method of media texts monitoring, the comparative method; the method of contextual analysis, the methods of functional analysis. Results. Anthroponyms in media texts contribute to the exact reproduction of facts, the display of a certain time-space. The use of an anthroponym in the media gives its bearer greater social significance; silencing an anthroponym demonstrates a desire to remove its bearer from the public agenda. Anthroponyms can reflect person’s social connections, inform about a belonging to a certain national, ethnic, age, social group. Conclusions Anthroponyms give media text more credibility, because they inform about a specific person in specific realities, personalize information. Anthroponyms are capable to mark time-space, therefore the actualization of proper names can be a means of transferring to another time, informing about forgotten historical facts and persons. Given the ability of anthroponyms – the names of famous persons – to be reduced, the journalist should take into account the possible difficulties of identifying such a person in a different time-space or under the condition of insufficient recognition. Entering the language game, anthroponyms are actualizing simultaneously meanings associated with different time-spaces, such ability can be effectively used to draw historical or cultural parallels, create an expressive load. Given the ability of anthroponyms to increase or decrease social status, journalists should be responsible in the selection of proper names as part of the factual material of the media text. Marking through anthroponyms the connection with national, social, age groups makes these words unique identifiers of the division into “own” or “strangers”, demonstrates the attitude of the speaker towards the bearer of his own name. Significance. The revealed functional parameters of anthroponyms as part of the actual material of the media text provide journalists with ample opportunities for the implementation of various communicative tasks. Key words: media text, anthroponym, factual material, language picture of the world, time-space, social communications.
ALTERAÇÕES CLÍNICAS IDENTIFICADAS EM CRIANÇAS APÓS INFECÇÃO PELO ZIKA VÍRUS: SCOPING REVIEW
Authors: Rodrigues Rafaela Carla Piotto , UJehara Silvia Carla da Silva André , Vicente Lígia Marques
Published: March 2024

Objetivo: Mapear quais foram as evidências científicas sobre as alterações clínicas presentes em crianças portadoras de microcefalia oriunda da infecção pelo Zika Vírus durante o período da gestação. Método: Trata-se de uma revisão de escopo, onde, as buscas foram realizadas nas bases de dados Lilacs, PubMed, SciELO e Web of Science. Resultados: Foram selecionados ao todo, 36 artigos. Os estudos apresentaram as alterações clínicas que foram tidas como as mais comuns e prevalentes em crianças portadores de microcefalia. Os déficits mais comuns entre elas eram na parte neurológica, respiratória ou motora, a depender da gravidade, déficit também nos marcos de desenvolvimento que são considerados presentes em uma criança sem essa doença, chegando até sinais de maior gravidade como as convulsões. Conclusão: O recém-nascido, assim como a criança, portadora de microcefalia necessita de uma atenção especial por parte de uma equipe multidisciplinar, com vistas a minimizar os possíveis danos ocasionados em sistemas neuromusculares e psicomotores.
FORMATION FEATURES OF DIFFUSION LAYER OF STEEL VNS 32 — VI CONTAINING BERYLLIUM IN CASE OF VARIOUS TYPES OF CHEMICAL-THERMAL TREATMENT
Authors: Sevalnyov1 G.S. , Dvoretskov1 R. M. , Nefyodkin1 D. Yu. , Gorbovets1 M. A. ,   1National Research Center «Kurchatov Institute» — All-Russian Scientific Research Institute of Aviation Materials (VIAM), Moscow, 105005, Russian Federation, Kolmakov2 A. G. ,   2A.A. Baikov Institute of Metallurgy and Materials Science (IMET RAN), Russian Academy of Sciences, Moscow, 119334, Russian Federation, Solntseva3 A. A. ,   3Bauman Moscow State Technical University (National Research University), Moscow, 105005, Russian Federation., 32
Published: March 2024

A study of the formation features of a diffusion layer in VNS32 — VI steel containing beryllium during vacuum nitriding and carburizing has been carried out. It was established that saturation with carbon and nitrogen made it possible to remove delta ferrite from the structure. The successive combination of nitriding and carburizing due to the formation of a structure more favorable for the diffusion of interstitial elements on the surface of the samples provides an increase in the total thickness of the diffusion layer by 5—10 times more as compared to the thickness values for individual treatments.
The Protective Effects of Ecdysterone on Cognitive Impairment through Regulating Akt/GSK-3β/Nrf2 Signaling Pathway and Oxidative Stress in Cognitive Mice Model and Aβ-Induced Cell Neurotoxicity
Authors: Xing Haihui , Chen Guohua , Raza Faisal , Zafar Hajra , Xing Lei , Li Qiaoyun , Li Ran , Xu Shanshan , Chen Dan
Published: March 2024

Background: Severe neurological condition like Alzheimer’s disease (AD) has a significantly negative impact on families and society, wherein there is no proven cure. As one of the principal active constituents of Achyranthes bidentata Blume, ecdysterone (ECR) has demonstrated antioxidant and cognitive dysfunction improvement effects. Nonetheless, the mechanism underlying the improvement of cognitive dysfunction by ECR remains unclear. This study sought to ascertain whether ECR may allebviate cognitive impairment by reducing oxidative stress via activation of the nuclear factor erythroid-2-related factor-2 (Nrf2) antioxidant system through Akt/GSK3β pathway. Methods: In terms of the experimental procedure, we determined the neuroprotective benefits of ECR in vivo via a cognitive impairment model of senescence-accelerated mouse prone 8 (SAMP8), we performed procedures such as behavioral testing, biochemical assaying, Nissl and TUNEL stainings, as well as flow cytometry, immunohistochemistry and western blotting. Furthermore, we investigated the underlying mechanistic action of ECR by activating PC12 cells with β-amyloid peptide fragment 25-35 (Aβ25-35). Results: In vivo studies showed that ECR effectively improved cognitive impairment in SAMP8 via enhancement of learning and memory capabilities, but decreased oxidative stress, apoptosis and neuronal damage in the hippocampus. During the in vitro study, we observed that ECR dose-dependently reduced the oxidative stress and apoptosis that were induced in PC12 cells by Aβ25-35. Additionally, the use of Akt inhibitors further established the potential of ECR to control Nrf2 through activation of the Akt/GSK3β pathway and protect the PC12 cells from Aβ25-35 induced damage. Conclusions: These findings offer proof that ECR reduces cognitive impairment by triggering the Nrf2 antioxidant system via the Akt/GSK3β pathway and offer fresh information on ECR’s potential as a promising therapeutic development candidate for AD.
Is Osteoarthritis a Vascular Disease?
Authors: Olansen Jon , Dyke Jonathan P. , Aaron Roy K.
Published: March 2024

Osteoarthritis (OA) is now considered as a multifaceted disease affecting various articular tissues, including cartilage, bone, synovium, and surrounding ligaments. The pathophysiology strongly implicates intricate chemical communication, primarily through cytokines, leading to the production of degradative enzymes in cartilage, inflammatory peptides in synovium, and structural changes in bone, resulting in characteristic clinical features such as joint deformities and loss of cartilage space seen on X-rays. Recent studies highlight the previously underestimated role of subchondral bone in OA, revealing its permeability to cytokines and raising questions about the influence of abnormal perfusion on OA pathophysiology, suggesting a vascular component in the disease’s etiology. In essence, alterations in bone perfusion, including reduced venous outflow and intraosseous hypertension, play a crucial role in influencing the physicochemical environment of subchondral bone, impacting osteoblast cytokine expression and contributing to trabecular remodeling, changes in chondrocyte phenotype, and ultimately cartilage matrix degeneration in OA. Dynamic contrast (gadolinium) enhanced magnetic resonance imaging (DCE-MRI) was used to quantify perfusion kinetics in normal and osteoarthritic subchondral bone, demonstrating that decreased perfusion temporally precedes and spatially correlates with cartilage lesions in both young Dunkin-Hartley (D-H) guinea pigs and humans with osteoarthritis. Pharmacokinetic analysis of DCE-MRI generated data reveals decreased tracer clearance and outflow obstruction in the medial tibial plateau of osteoarthritic guinea pigs, coinciding with progressive cartilage degradation, loss of Safranin O staining, and increased expression of matrix metalloproteinases and interleukin-1. Positron emission tomographic (PET) scanning using 18F-Fluoride reveals a relationship among bone blood flow, cartilage lesions, and 18F-Fluoride influx rate in OA, highlighting the intricate relationships between decreased perfusion, altered bone metabolism, and the progression of osteoarthritis. These findings, supported by 18F-Fluoride PET data, suggest the presence of venous stasis associated with outflow obstruction, emphasizing the role of decreased subchondral bone perfusion in the pathophysiology of OA and its association with reduced osteoblast activity and advanced cartilage degeneration.
Neuropeptides as Potential Biomarkers in Vascular Dementia
Authors: Yan Xue , Ma Yihong , Yan Limin , Li Simin , Xu Yuzhen
Published: March 2024

Neuropeptides are endogenous active substances within the central and peripheral nervous systems that play important roles in a wide range of brain functions, including metabolism, food intake, social behavior, reproduction, learning, sleep, and wakefulness. This article reviews recent advances in the involvement of neuropeptides in vascular dementia. Neuropeptides are present in the brain as chemical signals and last for nearly 50 years. Peptide hormones are chemical signals of the endocrine system. Thus, neuropeptides are the most diverse class of signaling molecules in the brain, involving the genomes of many mammals, encoding neuropeptide precursors and many bioactive neuropeptides. Here the aim is to describe the recent advances in classical neuropeptides, as well as putative neuropeptides from other families, in the control of or as diagnostic tools for vascular dementia. Additionally, its molecular mechanisms are described to explore new avenues of treatment and early diagnosis, as there is increasing evidence that dysregulation of vascular processes is associated with different pathological conditions.