bluuebunny/crossref_metadata_2025 huggingface dataset:
Future biology teachers’ opinions on technological pedagogical content knowledge
Authors: Nogerbek Assem , Sumatokhin Sergey , Maimatayeva Assiya , Ziyayeva Gulnar , Childibayev Dzhumadil
Published: April 2022

The purpose of this research is to evaluate the technological pedagogical content knowledge of future biology teachers. In this study, the qualitative research method was used while making associations about the findings. Twenty (20) students studying in the biology teaching department of various universities in Kazakhstan constitute the study group of the research. The data of the research were collected by semi-structured interview technique. Considering the purpose of the research, a semi-structured interview form developed by the researcher was prepared. As a result of the research, it was determined that the majority of the pre-service teachers found themselves somewhat inadequate regarding their teaching skills by suitably combining technology and teaching approaches. The vast majority of pre-service teachers find themselves inadequate regarding their ability to use information and communication technologies as a tool for students to share their thoughts and ideas.

Keywords: Content knowledge; pedagogical knowledge; technology knowledge.
Exploring Determinants of Desirable Financial Behaviors Using Decision Tree Analysis Evidence From Four Waves of National Financial Capability Study
Authors: Lux Don , Kauzlarich Laura
Published: April 2022

The purpose of this article is to utilize decision tree (DT) analysis to examine the relationship between income level, financial satisfaction, financial confidence, financial knowledge, and several demographics with a goal of better understanding desirable financial behavior. The emphasis of this analysis is focused particularly upon better understanding the role of financial knowledge in desirable behavior outcomes. DT analysis is most useful when an analysis includes numerous variables and solving problems where the cumulative learning process is inherent. Our DT analysis of four FINRA National Financial Capability datasets (2009, 2012, 2015, and 2018) suggest that financial knowledge is a relevant variable only under specific circumstances and for respondents with relatively higher income levels. Key variables in the DT analysis included income level and financial satisfaction.
Tanto mar
Authors: Braga Ana Gabriela
Published: April 2022

Este artigo é um dos frutos da pesquisa “Maternidade encarcerada sob uma perspectiva comparada” que teve como objetivo analisar o exercício da maternidade em condições de privação de liberdade numa prisão de mulheres em Portugal a partir da experiência no sistema prisional brasileiro. Por meio da metodologia etnográfica- com técnicas combinadas de observação participante, entrevistas, análise documental e projeção de filmes com grupos de conversa- realizei o trabalho de campo junto às mulheres e crianças presas na “Casa das Mães”, no Estabelecimento Prisional de Tires. Neste texto, reúno notas metodológicas que contam sobre minha entrada, percursos e localizações no campo de pesquisa, buscando enquadrá-las dentro de um panorama mais geral dos arranjos de poder na prisão e posicionalidades na produção empírica dos estudos prisionais.
[Book Review] A Pocket Guide to Online Teaching: Translating the Evidence-Based Model Teaching Criteria, by Aaron Richmond, Regan Gurung, and Guy Boysen
Authors: Hakala Chris , Salvatore Gabrielle , Ames Jacob
Published: April 2022

A book review of A Pocket Guide to Online Teaching: Translating the Evidence-Based Model Teaching Criteria, by Aaron Richmond, Regan Gurung, and Guy Boysen.
The Difficulties of Trabeculectomy in a Primary Angle-Closure Glaucoma Patient
Authors: Ramadhani Herdina , Nurwasis Nurwasis , Primitasari Yulia , Komaratih Evelyn
Published: April 2022

Introduction: Trabeculectomy is an incisional surgery for glaucoma patient. Performing trabeculectomy in primary angle-closure glaucoma (PACG) is quite difficult. Caution is needed for the occurrence of flat anterior chamber (FAC) and malignant glaucoma. We present a case management of trabeculectomy in a PACG patient. Case presentation: A 52-year-old male had blurred vision in his left eye since one year ago. The patient felt left visual field narrowing since eight months ago. Patient had type 2 diabetes mellitus. Visual acuity on the left eye was 5/7.5. Intraocular pressure (IOP) increased (29 mmHg). Gonioscopy showed closed angle and peripheral anterior synechiae. Glaucomatous optic neuropathy was found with C/D ratio value of 0.9 and superior-inferior RNFL thinning on optical coherence tomography (OCT). Anterior segment OCT revealed shallow anterior chamber depth (ACD) 2.54 mm. The right eye visual acuity was 5/5, normal IOP, and shallow ACD (2.66 mm). Patient was underwent left eye trabeculectomy. Postoperatively, left eye anterior chamber and bleb were formed. He had normal IOP without glaucoma medications. Conclusions: Trabeculectomy is a surgical procedure for glaucoma. Close monitoring is required to avoid the risk of postoperative FAC and malignant glaucoma in PACG.
Influencia de la imagen de las tiendas de alimento del canal moderno en el valor de la marca propia en Lima Metropolitana
Authors:   ,   Universidad San Ignacio de Loyola, Bozzo Erazo Laura Rocio
Published: April 2022

El propósito de este estudio es examinar la influencia que tiene la imagen de la tienda del canal moderno en el valor de las marcas propias de Lima Metropolitana. Esta relación puede ser aprovechada por las cadenas de retails para aumentar la proposición de valor del mercado al que pertenece. Partiendo de una revisión de la literatura este trabajo revisa los conceptos de imagen de tienda y el valor de la marca propia del distribuidor, se estudia una muestra de 420 amas de casa en un periodo de dos años. Los resultados nos dan a conocer que en el mercado de Lima Metropolitana la imagen de la tienda y el valor de la marca propia están relacionados únicamente por la variable precio, en contraposición con los resultados obtenidos en otras ciudades de países desarrollados en donde la asociación de estos dos constructos está relacionada a las dimensiones de la imagen estratégica e imagen comercial del establecimiento donde se comercializan los productos de marca privada. En la actualidad, los negocios minoristas son cada vez más conscientes de la importancia que tiene su capacidad para vender más y mejor, razón por la cual ha sido necesario el desarrollo de marcas de venta exclusiva que les permita llegar a los consumidores con precios más económicos y sin perjudicar su rentabilidad. Es así que, la creación de las marcas propias se ha vuelto una tendencia que cobra fuerza en este sector y Perú no es la excepción. Aunque esta tendencia, brinda una oportunidad de negocio, en la actualidad no se ha estudiado este fenómeno en Lima Metropolitana, por lo tanto, existe la oportunidad de ayudar a las tiendas de alimentos del canal moderno a formular estrategias que ayuden a incrementar la propensión del consumo de este sector.
Estrategias didácticas para desarrollar el pensamiento crítico en estudiantes del nivel inicial de una institución educativa del distrito de Puente Piedra, Lima
Authors:   ,   Universidad San Ignacio de Loyola, Asencios Vasquez Santa Pelagia
Published: April 2022

Este trabajo de investigación titulado “Estrategias didácticas para desarrollar el pensamiento crítico en los estudiantes del nivel inicial, del distrito de Puente Piedra, Lima” tuvo como objetivo proponer una estrategia didáctica para desarrollar el pensamiento crítico de los niños y niñas del nivel inicial de este distrito de Lima Norte en estudio. La metodología se ampara en el paradigma socio crítico, de enfoque cualitativo, tipo aplicada educacional, de diseño no experimental. La muestra es de 30 docentes de la UGEL 04 de Comas y la unidad de análisis lo conforman docentes y expertos en educación inicial. Los resultados demostraron que para el desarrollo del pensamiento crítico de los estudiantes los docentes tienen un limitado conocimiento de estrategias y si las conocen las usan inadecuadamente. Asimismo, no existe una atención y motivación al docente este tiempo de crisis de salubridad. Ante lo expuesto, se proponen estrategias que beneficien al docente en su formación estratégica -educativa, así como también se elabore un plan de atención socio-emocional para ellos.
STRATEGI KOMUNIKASI PELAYANAN PUBLIK MELALUI E-GOVERNMENT DI PUSAT PENGELOLAAN PENGADUAN MASYARAKAT (P3M) KOTA SEMARANG
Authors: Fazarusda Azka , Indrayani Heni
Published: April 2022

Public services is a service that provided by the government to the community. In general, the public services deliver the benefit to the people in a particular society or community. Semarang city government contribute the public services in the form of management of public complaints responsible by Community Complaints Management Center (P3M) as a part of Diskominfo. P3M is optimize the various media channels or e-Government such as Instagram, Twitter, WhatsApp, Telegram, Web and SMS. Meanwhile, to advance the media channel to maximize the communication, people tend to use WhatsApp to convey the complaint. According to data on the Diskominfo Semarang city there are 515 complaints which status has not been in the process of the total complaint 2558 in  January to August 2019. The aims of this study is to determine the public services communication strategy by e-Government.  This research is used a paradigm of constructivism, qualitative research methods with a case study approach. This study uses social systems theory and political system models. The results of the study showed that there was an attachment between the political system in P3M, namely “Lapor Hendi” System and the social system theory. Lapor Hendi is a non-bulkhead service that is a public service communication strategy between the government and the community. Communities in the Semarang City area are free to give complaints, suggestions and criticisms to the government directly. The stages of e-Government communication strategies begin with fact finding, namely the filtering of complaints that are followed up. Second, planning based on the legal basis, namely mayor regulations, work plan planning and strategic plans. Third, action and communication e-Government, namely the optimization of media channels by S4PN and the report system system. Finally, the evaluation shows that there are many complaints from the public through media channels or e-Government and become the material for the next City Government policy strategy
Dukungan Program Kota Layak Anak melalui Perancangan Konten Siaran Streaming Radio Anak “KONATA”
Authors: Arzil Andrine Prima Afneta , Ayu Aulia Suminar , Haryono Novita , Hastjarjo Sri , Widyantoro Widyantoro
Published: April 2022

Surakarta strives to create a child-friendly city ecosystem. Communication, Informatics, Statistics, and Encryption Department of Surakarta provides Konata (Komunitas Anak Surakarta – Surakarta Kids Forum) as the only kids radio in Indonesia. Konata uses the radio streaming format which contains  the actual issues, songs, stories, fairy tales, and bring talented children without leaving a distinctive element of children. It also held roadshow at schools. The radio can survive because of the important role of its announcers. Therefore, it requires to develop their technical and content creating skill. In this pandemic era, we’ll hold online workshop for the Konata’s announcers in collaboration with Solopos and  Education and Cultural Department of Surakarta. Our program is going to target the elementary school teachers as participants.  We hope this program can  synergize the triple helix between university, governmental institution, and community to serve them in term of social service.
LABOR MARKET REQUIREMENTS AND KEY TRENDS IN THE FORMATION OF “QUALITY” FUTURE SPECIALIST OF THE SECOND AND THIRD LEVELS OF PREPARATION FOR PREPARATION SPECIALTIES 011 “EDUCATIONAL, PEDAGOGICAL SCIENCES”
Authors: Romanovskiy Olexandr , Ihnatiuk Olha
Published: April 2022

the article is dedicated to the topical problem of forming a “quality” future teacher-leader in the conditions of Master’s and postgraduate studies under the specialty 011 “Educational, pedagogical sciences”. The essence of the term “quality seeker for higher education” in the dimension of the theory and practice of vocational education is defined. Based on the defined labor market requirements to the skills of modern specialists in higher education and experimental data on the experience of undergraduate and graduate students training under the specialty 011 “Educational, pedagogical sciences” in NTU “KhPI”, key trends in future lecturers training are identified and substantiated. It is proposed to consider and implement in the university the prospect of increasing the level of professional competence of lecturers of non-pedagogical profiles.
FORMATION OF WAYS OF ROLE INTERACTION OF FUTURE LEADERS IN THE LEARNING PROCESS AS AN IMPORTANT CONDITION FOR THEIR SUCCESSFUL ADAPTATION TO THE PROFESSIONAL ACTIVITY
Authors: Нura Tatyana , Mykhaylychenko Valentyna
Published: April 2022

the meaning of the terms “manager”, “manager’s role” and “role interaction” is revealed in the article. It is shown that the process of successful adaptation to the professional activity of future leaders is based on leading personal innovations, the formation of which is necessary in the process of studying in higher education institutions. The results of a study in which the characteristics of their emotional, communicative and regulatory qualities were considered are noted in the article. The obtained results allow us to conclude that the future leaders lack a number of qualities that contribute to their successful adaptation to professional management activity. Their formation requires improvement of methods, tools and organizational forms that ensure this process.

The use of role-playing games in the form of video materials and presentations plays an important role in the training sessions. The objectives, principles and role of training as a methodological tool to enhance the adaptation of future leaders to successful professional activity are highlighted in the article.
PROFESSIONAL CULTURE IN THE TEACHER’S INTELLIGENCE SYSTEM
Authors: Kipenskiy Andriy , Romanovskiy Olexandr , Ponomaryov Olexandr
Published: April 2022

the article is devoted to the study of the role and place of professional culture of a teacher in the general system of his intelligence. Intelligence itself appears as a holistic formation and systemic unity of its multifactorial and multifunctional nature of activity and personality of the teacher, his professionalism, general and professional culture. The intelligence of the teacher is one of his important personal characteristics and a powerful source of purposeful pedagogical influence on students. In addition, the intelligence of the teacher is a significant stimulus to his constant self-development and self-improvement. The importance of professionalism and professional culture as integral parts of the intelligentsia and the teacher and his students is confirmed by the results of experimental research.
Students’ Perception on the Implementation of Online Learning in Kubutambahan District by Using Input and Outcome Evaluation
Authors: Gede Suadnya Suastika
Published: April 2022

Covid-19 makes big problems especially in education. Students have an important role in education especially in online learning because they follow the online learning provided by the teacher. Based on the observation, students are afraid and embarrassed because of less attention from the students in online learning. This research aims to identify the students’ perception on the implementation of online learning in Kubutambahan District by using input and outcome evaluation, so that students can tell their problems in the online learning process. This research is descriptive qualitative which uses observation, questionnaire, and interview as data collection instruments. Input and outcome evaluation are the guidelines in designing the questionnaire and interview. The research results show that the students stated that assessment and schools’ facilities and infrastructures in SMPN 2 Kubutambahan and SMPN 5 Kubutambahan support them on the implementation of online learning although most of them have never followed socialization.
EQUATING METHOD FOR LEARNING OUTCOMES OF ELEMENTARY SCHOOL/MADRASAH STUDENTS
Authors: Iriyadi Deni
Published: April 2022

This study aims to determine the method of equalizing a good score on a small number of items that are often found at the elementary/madrasah level. This research is a simulation study conducted by comparing capabilities in terms of three distributions. The sample size used in this study was 50 responses for the distribution of the ability of each group (normal, positive skewness, and negative skewness). Replication was carried out in 50 for each ability group using the help of Wingen3. The indicator used to evaluate the results of the equation is the Root mean squared error. The results showed that groups with the same ability skewness distribution (normal ability distribution - normal ability distribution, positive skewness ability distribution - positive skewness ability distribution, and negative skewness ability distribution - negative skewness ability distribution) would give a lower RMSE score than groups with different ability distributions. A low RMSE value indicates that the error of the measurement results is low
Il caso e la necessità: lettura del Canto XIII dell’Inferno
Authors: Prosperi Carlo
Published: April 2022

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Review of: Riccardo Gandolfi, Le vite degli artisti di Gaspare Celio. ‘Compendio delle vite di Vasari con alcune altre aggiunte’, Leo S. Olschki, Firenze 2021, 390 pp., ISBN 978882226702 3.
Authors: Molina Barea María del Carmen
Published: April 2022

Review of:

Riccardo Gandolfi, Le vite degli artisti di Gaspare Celio. ‘Compendio delle vite di Vasari con alcune altre aggiunte’, Leo S. Olschki, Firenze 2021 (Biblioteca dell'Archivum romanicum, serie I: Storia, letteratura, paleografia, 504), 390 pp., ISBN 978882226702 3.
A Thirteenth-Century Latin List of Ptolemaic Coordinates
Authors: Nothaft Carl Philipp Emanuel
Published: April 2022

This article makes the argument that a Latin table of geographic coordinates, copied in Italy in the second half of the thirteenth century (MS Florence, Biblioteca Medicea Laurenziana, Ashburnham 211, fol. 260r), is derived from a list excerpted from a Greek manuscript of the Κανὼν πόλεων ἐπισήμων (« Table of important cities ») in Ptolemy’s Handy Tables. Other sources that appear to bear witness to a thirteenth-century dissemination in Italy of geographic data from the Κανὼν include the Summa de astris of Gerard of Feltre (1264/65) and some later coordinate lists contained in fourteenth-century manuscripts.
L’'esegesi topica' di Pietro Abelardo nel Commento all’Epistola ai Romani (11,16-24)
Authors: Magnano Fiorella
Published: April 2022

Sebbene molti contributi abbiano posto in evidenza la struttura dialettica presente nelle opere teologiche di Abelardo, sorprende la completa assenza di studi relativi all’impiego dei luoghi dialettici a servizio del discorso teologico. Pertanto, il presente studio intende avviare una riflessione in merito a questo specifico aspetto dell’opera del maestro palatino, allo scopo di illustrare la modalità con cui i luoghi dialettici esposti in sede logica siano stati successivamente messi a servizio dell’esegesi del testo sacro. Le mie conclusioni sono che ‘l’esegesi topica’ esprime lo spazio di autonomia assegnato alla ragione umana in cerca di un fondamento, se non razionale almeno ragionevole, dell’alto grado di verosimiglianza di quanto trasmesso dalla Rivelazione.
The Statistics of the Classics in Thirteenth-Century Italy
Authors: Monfasani John
Published: April 2022

In 2015 I argued that the statistics of manuscripts derived from Élisabeth Pellegrin’s Les manuscrits classique latins de la Bibliothèque Vaticane proved that interest in classical literature did not collapse in thirteenth-century Italy as it did in Northern Europe. In 2017 Robert Black published a refutation of my argument. The present article is an answer to Black’s article.
Review of: Paolo Pellegrini, Dante Alighieri: Una vita, Giulio Einaudi, Torino 2021 (Piccola Biblioteca Einaudi, nuova serie, 747: Saggistica letteraria e linguistica), XXII + 252 pp., ISBN 9788806247218.
Authors: Barnes John C.
Published: April 2022

Review of:

Paolo Pellegrini, Dante Alighieri: Una vita, Giulio Einaudi, Torino 2021 (Piccola Biblioteca Einaudi, nuova serie, 747: Saggistica letteraria e linguistica), XXII + 252 pp., ISBN 9788806247218.
In memoriam Herbert A. Davidson (25.V.1932–28.IV.2021)
Authors: Puig Montada Josep
Published: April 2022

In memoriam

Herbert A. Davidson

(25.V.1932–28.IV.2021)
Ragione e rigenerazione: Studio sul De originali peccato di Agrippa
Authors: Gurashi Dario
Published: April 2022

Heinrich Cornelius Agrippa’s reading of Original sin is based on a philosophical approach: in the declamation De originali peccato he explains the Fall as a metaphor for sexual intercourse and associates each Biblical character with a faculty of the soul: Adam embodies faith, Eve portrays reason, and the Serpent represents sexual desire. By deliberately disobeying God, Adam and Eve reject chastity and claim the divine power of giving life. Their transgression is therefore rooted in the presumption of overcoming the limits of human nature through concupiscence. Moving from the connection between theology and sexuality, Agrippa examines the role of man/faith and woman/reason, which symbolize existential choices aiming at the spiritual redemption of humankind.
From Venice to Cairo: Notes from an Early 16th-century Voyage Across the Mediterranean
Authors: Benedetti Laura
Published: April 2022

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'Quididad', 'definición' y 'partes de la definición' en Dietrich de Freiberg: una interpretación de Metafísica Zeta, 10
Authors: Ocampo Fernanda
Published: April 2022

Este trabajo se propone considerar la doctrina de la ‘quididad’ y la ‘definición’ en Dietrich de Freiberg, en relación con una de sus cuatro tesis medulares: a saber, que la quididad de la substancia compuesta no está constituida sino por la forma substancial, y que son las partes de dicha forma (y no otras) las que la definición significa a través de los términos que la integran. En este sentido, buscaremos evidenciar los elementos doctrinales que motivan la posición de Dietrich, haciendo a la vez hincapié en su modo peculiar de leer a Aristóteles, especialmente en lo que se refiere a las tesis centrales esbozadas en Metafísica VII 10.
Specters of Pico: A Note Concerning a Recent Book on the Oratio de dignitate hominis
Authors: Burzelli Luca
Published: April 2022

Review article of:

Brian Copenhaver, Magic and the Dignity of Man: Pico della Mirandola and his ‘Oration’ in Modern Memory, Belknap of Harvard University Press, Cambridge, MA–London 2019.
In memoriam David Luscombe (22.VII.1938–30.VIII.2021)
Authors: Dunne Michael
Published: April 2022

In memoriam

David Luscombe

(22.VII.1938–30.VIII.2021)
Review of: Nicola Polloni, The Twelfth-Century Renewal of Latin Metaphysics: Gundissalinus’s Ontology of Matter and Form, Institute of Medieval and Early Modern Studies – PIMS, Durham–Toronto 2020, XIII+318 pp., ISBN: 9780888448651.
Authors: Saccenti Riccardo
Published: April 2022

Review of:

Nicola Polloni, The Twelfth-Century Renewal of Latin Metaphysics: Gundissalinus’s Ontology of Matter and Form, Institute of Medieval and Early Modern Studies–Pontifical Institute of Mediaeval Studies, Durham–Toronto 2020 (Durham Medieval and Renaissance Monographs and Essays, 6), ISBN: 978-0-88844-865-1.
Review of: Josef Stern, James T. Robinson, Yonatan Shemesh (eds.), Maimonides’ ‘Guide of the Perplexed’ in Translation. A History from the Thirteenth Century to Twentieth, Chicago University Press, Chicago, ILL. 2019, VII + 481 pp., ISBN 9780226457635.
Authors: Mantas-España Pedro
Published: April 2022

Review of:

Review of: Josef Stern, James T. Robinson, Yonatan Shemesh (eds.), Maimonides’ ‘Guide of the Perplexed’ in Translation. A History from the Thirteenth Century to Twentieth, Chicago University Press, Chicago, ILL. 2019, VII + 481 pp., ISBN 9780226457635.
Law and art in the female resistance to patriarcal authoritarianism during the brazilian civil military dictatorship
Authors: Godoy Arnaldo Sampaio de Moraes , Braga Raquel Xavier Vieira
Published: April 2022

The relationship between law and art – apparently subtle – is intense. Law is a cultural product resulting from human conventions, while art, in its progressive bias, is a cultural product that questions these conventions to deliver life from the prisons that human beings create. The avant-garde artistic field contributes to breaking paradigms, as well as the female gender, which is constantly emancipated to deconstruct paradigmatic meanings. The woman increasingly comes out of silence and reports her experiences. From the perspective of law, there are very important historical events that can become institutionalized. The alteration of constitutional norms, depending on the context, can represent the gateway to authoritarian models filled with arbitrariness, such as, for example, the civil military dictatorship of 64 and, in particular, the AI-5, in Brazil. In the arrangement between law, art and gender issues, a powerful context for reflection appears. The hypothesis presented is that art is capable of converting tragedies into powers and, exposing the experience suffered by women during the Brazilian military regime in literature and cinema, it acts on the possibility of society overcoming traumatic experiences, while mobilizing the right to adapt to new models of human interaction, enabling – or at least not hindering – the nascent positions of female subjects in the social field. The research starts from primary and secondary legal sources, such as the analysis of historical documents, news published by the major newspapers of the time, examination of normative texts and reading of classic works.
Dietary total antioxidant capacity in relation to metabolic health status in overweight and obese adolescents
Authors: Mohammadi Sobhan , Lotfi Keyhan , Mirzaei Saeideh , Asadi Ali , Akhlaghi Masoumeh , Saneei Parvane
Published: April 2022

Background: Although several studies evaluated the relationship between individual dietary antioxidants and metabolic health condition, data on the association between dietary total antioxidant capacity and metabolic health among children and adolescents is limited. We investigated the relation between dietary total antioxidant capacity and metabolic health status in Iranian overweight/obese adolescents.Methods: This cross-sectional study was conducted on 203 overweight/obese adolescents. Dietary intakes were evaluated by a validated food frequency questionnaire. Ferric Reducing-Antioxidant Power (FRAP) was considered to indicate dietary total antioxidant capacity. Anthropometric parameters and blood pressure status were measured. Fasting blood samples were obtained to determine circulating insulin, glucose and lipid profile. Two different methods (modified International Diabetes Federation (IDF) criteria and IDF criteria along with insulin resistance) were applied to classify participants as metabolically healthy obese (MHO) or unhealthy obese (MUO).Results: According to IDF and IDF/HOMA definitions, a total of 79 (38.9%) and 67 (33.0%) adolescents were respectively defined as MUO. Considering IDF criteria, highest tertile of FRAP was related to lower odds of being MUO in the maximally-adjusted model (OR: 0.40; 95%CI: 0.16-0.96), compared to the lowest tertile. However, based on the IDF/HOMA-IR criteria, no significant relation was found between FRAP and odds of MUO (OR: 0.49; 95%CI: 0.19-1.23) after considering all possible confounders.Conclusions: We found that adolescents with the higher intakes of dietary antioxidants have lower possibility of MUO based on IDF criteria. However, no substantial relation was found considering HOMA-IR/IDF definition. Further prospective cohort studies need to be done to confirm these findings.
Lower genetic diversity and decreased genetic differentiation in populations of western flower thrips across an invaded range
Authors: Sun Li-Na , Cao Li-Jun , Chen Jin-Cui , Ma Li-Jun , Zhang Gui-Fen , Wu San-An , Hoffmann Ary Anthony , Wei Shu-Jun
Published: April 2022

Colonizing populations of alien invasive species are often unstable due to their small population size, ongoing gene flow, and ongoing adaptation to local conditions. These processes should lead to molecular signatures at the population level. However, temporal changes in genetic patterns after introduction are rarely examined. The western flower thrips (WFT), Frankliniella occidentalis (Pergande), is a globally invasive pest of vegetables and ornamental crops. Early colonized populations in China exhibited a strong population structure reflecting different invasion sources. Here, we re-examine this pattern after ten years. Over this period, the number of alleles has declined significantly and strong population genetic structure found early in the invasion stage has been lost. A high level of gene flow between both geographically close and distant populations was identified. The reduced allele number and loss of population structure might reflect both ongoing gene flow and ongoing control measures to suppress populations. Our results emphasize the importance of continuing to manage gene flow among invaded areas to limit the exchange of alleles that might facilitate further adaptive changes following invasion.
The saponin bomb: a nucleolar liquid-liquid phase-separated β-glucosidase hydrolyses triterpene saponins in Medicago truncatula
Authors: Erffelinck Marie-Laure , Mertens Jan , Lacchini Elia , Molina-Hidalgo Francisco Javier , Tzfadia Oren , Cárdenas Pablo , Pollier Jacob , Tava Aldo , Bak Søren , Goossens Alain
Published: April 2022

Plants often protect themselves from their own bioactive defense metabolites by storing them in less active forms. Consequently, plants also need systems allowing correct spatiotemporal reactivation of such metabolites, for instance under pathogen attack. Here we show that the model legume Medicago truncatula has evolved a two-component system composed of a β-glucosidase and triterpene saponins, which are physically separated from each other in intact cells. The β-glucosidase, which is stored in the nucleolus and subjected to liquid-liquid phase separation in intact cells, is released and united with its substrates only upon tissue damage, partly mediated by the surfactant action of the saponins themselves. Subsequently, enzymatic removal of carbohydrate groups from the saponins creates a pool of metabolites with an increased broad-spectrum antimicrobial activity. The evolution of this peculiar defense system benefited from both the intrinsic condensation abilities of the enzyme and the bioactivity properties of its substrates.
CRISPR/Cas9 editing of the MYO7A gene in rhesus macaque embryos to generate a primate model of Usher Syndrome type 1B
Authors: Ryu Junghyun , Statz John P. , Chan William , Burch Fernanda C. , Brigande John V. , Kempton Beth , Porsov Edward V. , Renner Lauren , McGill Trevor , Burwitz Benjamin J. , Hanna Carol B. , Neuringer Martha , Hennebold Jon D.
Published: April 2022

Mutations in the MYO7A gene lead to Usher syndrome type 1B (USH1B), a disease characterized by congenital deafness, vision loss, and balance impairment. To create a nonhuman primate (NHP) USH1B model, CRISPR/Cas9 was used to disrupt MYO7A in rhesus macaque zygotes. The targeting efficiency of Cas9 mRNA and hybridized crRNA-tracrRNA (hyb-gRNA) was compared to Cas9 nuclease (Nuc) protein and synthetic single guide (sg)RNAs. Nuc/sgRNA injection led to higher editing efficiencies relative to mRNA/hyb-gRNAs. Mutations were assessed by preimplantation genetic testing (PGT) and those with the desired mutations were transferred into surrogates. A pregnancy was established from an embryo where 92.1% of the PGT sequencing reads possessed a single G insertion that leads to a premature stop codon. Analysis of single peripheral blood leukocytes from the infant revealed that half the cells possessed the homozygous single base insertion and the remaining cells had the wild type MYO7A sequence. The infant showed sensitive auditory thresholds beginning at 3 months. Although further optimization is needed, our studies demonstrate that it is feasible to use CRISPR technologies for creating NHP models of human diseases.
Contributions of Cancer Treatment, Comorbidities, and Obesity to Aging-related Disease Risks among Non-Hodgkin’s Lymphoma Survivors
Authors: Ocier Krista , Abdelaziz Sarah , Kim Seungmin , Rowe Kerry , Snyder John , Deshmukh Vikrant G , Newman Michael , Fraser Alison , Smith Ken R , Porucznik Christina A , Shoaf Kimberley , Stanford Joseph B , Lee Catherine J , Hashibe Mia
Published: April 2022

Purpose. It is unknown whether cancer treatment or modifiable risk factors contribute more to the burden of long-term disease risks among B-cell non-Hodgkin’s lymphoma (B-NHL) survivors. Methods. B-NHL survivors were identified in the Utah Cancer Registry from 1997 to 2015. Population attributable fractions (PAF) were calculated to assess the role of clinical and lifestyle factors for 6 cardiovascular, pulmonary, and renal diseases. Results. Cancer treatment contributed to 11% of heart and pulmonary conditions and 14.1% of chronic kidney disease. Charlson Comorbidity Index (CCI) at baseline contributed to all six diseases with a range of 9.9% of heart disease to 26.5% of chronic kidney disease. High BMI at baseline contributed to 18.4% of congestive heart failure and 7.9% of pneumonia, while smoking contributed to 4.8% of COPD risk. Conclusion. Cancer treatment contributed more to heart disease, COPD, and chronic kidney disease among B-NHL survivors. High BMI at baseline contributed more to congestive heart failure and pneumonia than cancer treatment, whereas smoking at baseline was not a major contributor in this B-NHL survivor cohort. Baseline comorbidities consistently demonstrated high attributable risks for these diseases, demonstrating a strong association between preexisting comorbidities and aging-related disease risks.
Protective effect of Apolipoprotein E epsilon 3 on sporadic Alzheimer's Disease in Chinese population: A Meta-Analysis
Authors: Chen Qian , Chen Lei , Wang Ting , Kang Deying
Published: April 2022

Introduction Alzheimer's disease (AD) is fastly becoming one of the most expensive, deadly and burdensome diseases in this century. It has the fastest growing disease burden in China. Apolipoprotein E (APOE) polymorphic alleles are generally considered to be the primary genetic determinant of AD risk: individuals with the E4 allele are at increased risk of AD compared to individuals with the more common E3 allele. The association intensity is different among different ethnic groups, so a separate meta-analysis of the Chinese population is needed. Methods We searched Chinese and English databases to sift through literature over the past 20 years. Data on the APOE genotype and AD were collected for correlation analysis. OR was calculated according to APOE allele and genotype. Publication bias analysis and sensitivity analysis were performed, and the main results were further verified by subgroup analysis. Results The 116 eligible studies enrolled 23396 AD patients and 25568 healthy controls. The study subjects covered at least 30 of the 34 provincial-level administrative regions (including Taiwan). Partial sex ratio: AD male/female: 10291/11240; Control male/female: 11304/12428, X2=0.122，P=0.727. Meta-analysis results of alleles showed that I2>50% and Q statistics were significant for all genotypes, so the random effect model was selected. The frequency of the ApoE ε4 allele in AD was higher than that in healthy controls, and the difference was statistically significant (OR=2.847, 95% CI=[2.611-3.101], P<0.001). The frequencies of ApoE ε3 and ε2 in AD were lower than those in healthy controls, and the differences was statistically significant (ε3: OR=0.539, 95% CI=[0.504-0.576], P<0.001; ε2: OR=0.771, 95% CI=[0.705-0.843], P<0.001). The results of meta-analysis of AD genotype showed that ApoE ε2/ε4(OR=1.521, 95% CI=[1.270-1.823]，P<0.001)，ε3/ε4(OR=2.491, 95%CI=[2.267-2.738], P<0.001), ε4/ε4(OR=5.481, 95%CI=[4.801-6.257], P<0.001) allele genotype frequencies were higher than the healthy controls, The differences were all statistically significant. AD in ApoE ε2/ε2 (OR=0.612, 95% CI=[0.504-0.743], P<0.001), ε2/ε3 (OR=0.649, 95% CI=[0.585-0.714], P<0.001), and ε3/ε3 (OR=0.508, 95% CI=[0.468-0.551], P<0.001) was lower than that of healthy controls, and the differences was statistically significant. The results of the sensitivity analysis and subgroup analysis were consistent with those of the whole model. Conclusion The results provide support for the protective effect of the ApoE ε3/ε3 in developing AD. Discussion This research is the most comprehensive meta-analysis of the correlation between APOE and AD in the Chinese population by analysing the distribution of the APOE gene in AD reported in the last 20 years. It was concluded that the APOE ε3 allele had a protective effect on sporadic AD in the Chinese population with great significance, and its protective effect was stronger than that of ε2.
Knowledge, Attitudes and Practises of Dengue Prevention Between Dengue Sustained Hotspots and Non-Sustained Hotspots in Singapore: A Cross-Sectional Study
Authors: Pang Junxiong , Chng WeiQuan Jeth , Parvathi Tamilsalvan
Published: April 2022

Dengue sustained hotspots (SHS) have resulted in a significant public health burden. In our study, we aimed to evaluate knowledge, attitudes and practices (KAP) of dengue prevention between residents residing in dengue sustained hotspots and non-sustained hotspots (NSHS) in Singapore. A cross-sectional study with convenience sampling was conducted using digital survey in randomly selected SHS and NSHS residential areas. Inclusion criteria were residents aged 21 or older and residing in the same housing unit in 2019 and 2020. Chi-square test and T-test were used for comparison analysis of categorical and continuous variables, respectively. Single and multiple logistic regression were performed using Stata (version 13.1.226) with significance level alpha at P-value <0.05. A total of 466 respondents completed the self-administered, anonymous survey. Age, educational level, marital status, housing type, years staying in house, gross monthly income, hours at home (weekends) and hours of air-conditioning used (weekends) were significantly different between SHS and NSHS [P-value <0.05]. There were no significant difference in mean scores for Knowledge [SHS(24.66) vs. NSHS(24.37); P:0.18], Attitudes [SHS(10.38) vs NSHS(10.16); P:0.08] and Practices [SHS(9.27) vs NSHS(8.80); P:0.16] sections. Significant SHS-associated factors identified were age group 41–50 years old [Adjusted Odds Ratio (AOR):2.50; 95%CI:1.25-5.03], Malay (AOR:0.41; 95%CI:0.17-0.98), up to secondary school education (AOR:0.22; 95%CI:0.07-0.65), private condominium: AOR:1.99; 95%CI:1.17-3.39), residing in same household unit for 2-5 years (AOR:4.10; 95%CI:2.44-6.88), respondents who know that mosquito can breed in open container with stagnant water (AOR:0.25; 95%CI:0.06-0.98), disagree that reducing Aedes mosquitoes is the only way to prevent dengue: (AOR:1.88; 95%CI:1.19-3.00) and go to clinic/hospital even without severe symptoms: (AOR:0.61; 95%CI:0.39-0.95). These independently associated factors would be useful to strengthen community outreach campaign to reduce the risk of sustained dengue hotspots.
Instance segmentation for real-time video detection using FPN and Mask R-CNN
Authors: Yadav Anu , Kumar Ela
Published: April 2022

Instance segmentation of objects is a significant stage in real-time video detection. Object detection is the task of detecting the information of all types of objects in an image by marking the detected object's position in the image with a rectangular box. Using its great feature learning capabilities, deep learning attains a development in object detection research. Many researchers have performed object detection with different machine learning algorithms and focused on enhancing the accuracy of feature extraction. But the accuracy of the lower and higher-level features are not featured properly due to this error that occurred in the detection of the object at a lower level. To overcome this issue, FPN is used for feature extraction, which extracts both lower and higher-level features for accurate detection of an object, and the classification process is done by the proposed model (Mask R-CNN). The proposed model is implemented on python for finding the performance metrics such as accuracy, precision, execution time, error, etc. The accuracy for real-time object detection using FPN and Mask R-CNN is 97.8%, which is greater compared to the existing techniques such as TLD (Tracking-Learning detection), R-CNN, Fast R-CNN, Mask R-CNN and FPN. Thus, the designed model instantly segments the real-time video for object detection in an effective manner.
Lived experiences of a family-based lifestyle intervention for children with overweight and obesity: On sustainability, self-regulation, and program optimization
Authors: Putter Kaila C. , Jackson Ben , Thornton Ashleigh L. , Willis Claire , Goh Kong Min Bryce , Beauchamp Mark R. , Benjanuvatra Nat , Dimmock James A. , Budden Timothy
Published: April 2022

Background: Family-based lifestyle interventions (FBLIs) are an important method for treating childhood weight problems. Despite being recognized as an effective intervention method, the optimal structure of these interventions for children’s overweight and obesity has yet to be determined. Our aim was to better understand participants’ (a) capacity to self-regulate and achieve long-term outcomes, (b) perceptions regarding the optimal structure of FBLIs, and (c) insights into psychological concepts that may explain success of these programs.Methods: To understand participant experiences, we conducted focus group as well as one-to-one interviews with over 100 parents and children three months following their involvement in a 10-week, multi-component, FBLI involving resources, education, and experiential activities relating to healthy nutrition, physical activity, and behavior modification. Results: Thematic analysis identified challenges that can impair lasting behavior change (e.g., physical activity participation) following involvement in a FBLI. On optimizing these programs, participants emphasized fun, interactive content, interpersonal support, appropriate educational content, and behavior change techniques.Conclusions: Concepts rooted in motivational theory could help address calls for greater theoretical and mechanistic insight in FBLIs. Findings may support research advancement and assist health professionals to more consistently realize the potential of these interventions.
Quantitative soil erosion risk assessment due to rapid urbanization in the Cox’s Bazar district and Rohingya refugee camps in Bangladesh
Authors: Hossain Farhad , Kamal A S M Maksud , Sadeak Sumiya , Gazi Md. Yousuf
Published: April 2022

Soil erosion due to anthropogenic interventions is an emerging threat to the south-eastern coastal districts of Bangladesh. In the recent decade, land degradation intensified this process in this region due to the settlement of the Forcibly Displaced Myanmar Nationals (FDMNs) popularly known as ‘Rohingya’, who fled from Myanmar in 2017. The present study has attempted to quantify the regional soil loss (from 2015 to 2020) using the Revised Universal Soil Loss Equation (RUSLE) model. Results showed that the year 2015 and 2020 witnessed the mean soil erosion at a rate of around 58.2 and 59 t ha-1 yr-1, respectively in the Cox’s Bazar district in which 20 - 21% of the study area was highly subjected to soil erosion. But in the Rohingya camps mean soil erosion for these periods is 59 and 78 t ha-1 yr-1, respectively with a cumulative increase of 32%. The sub-districts adjacent to the Rohingya refugee camps (Ramu, Ukhia, and Teknaf) have experienced intense erosion compared to the others. From 2015 to 2020, a large increase of about 4.54 (6%) t ha-1 yr-1 is observed in the Palongkhali union whereas a maximum decrease of about - 8.08 (23%) t ha-1 yr-1 is observed in the Pokkhali union. In Rohingya camps, the worst soil erosion-affected areas are the camps 8E, 10, 14, 15, 16, and 17 having more than 100 t ha-1 yr-1 mean soil loss. Landuse change is the major contributing factor for soil erosion increase from 2015 to 2020 identified from the geographic detector method. The spatial variations are significantly controlled by slope and elevation factors. This work can help to understand the dynamics of soil erosion and the knowledge can be used for conservation planning in the Cox’s Bazar and Rohingya camp areas.
Study of La Candelaria streams water photocatalysis treatment over Lactuca sativa L seeds
Authors: Stella Andres , Coria Daniel , Alvarez Jesus , Muñoz Vicenta
Published: April 2022

Natural waters surface contain, among other compounds, humic and fulvic acids refractory to standard treatments, which are the main contributions to chemical oxygen demand (COD). To mineralize this organic matter heterogeneous photocatalysis (HP) has been used for the treatment of La Candelaria stream in Santa Fe (Argentina) close to wastewater treatment plant of the city, with a 9W low-pressure mercury lamp and TiO2 as photocatalyst. Aromaticity elimination has been followed by UV intensity at 254nm, and mineralization by analysis of COD. Effect of pH (7 − 5), TiO2 catalyst loading (500–1000 mg/L) and COD (17–120 mg/L) was studied in the treatment of these natural waters. The results reported optimal treatment conditions at pH 5 and TiO2 loading of 1000 mg/L. Biotoxicity tests were performed using Lactuca sativa L. seeds with the samples of natural waters containing 20, 48 and 76 mg/L COD, and the respective sample treated by photocatalysis in order to explore the effect of the photocatalytic treatment and over COD in media. While collected natural waters and the mineralized waters, after treatment, have non biotoxic behavior, the incomplete treated waters evidence a significant negative effect over germination rate (GR) and the elongation rate (ER), as consequence of concentration of compounds formed during the water photocatalytic treatment process. These results suggest as toxicology tests in outcome effluents to check efficiency of the water treatment plants should be necessary.