bluuebunny/crossref_metadata_2025 huggingface dataset:
Sensitivity and Reliability Analysis of Ultrasonic Pulse Parameters in Evaluating the Laboratory Properties of Asphalt Mixtures
Authors: Tan Xiaoshu , Wu Chunli , Li Liding , Li He , Liang Chunyu , Zhao Yongchao , Li Hanjun , Zhao Jing , Wang Fuen
Published: October 2023

The ultrasonic test is a promising non-destructive testing technique for evaluating the properties of asphalt mixtures. To investigate the applicability and reliability of ultrasonic testing technology (UTT) in evaluating the performance of asphalt mixtures, ultrasonic tests, indirect tensile tests, compression tests, and dynamic modulus tests were carried out at various temperatures. Subsequently, the distribution characteristics of ultrasonic traveling parameters for asphalt mixtures were analyzed. The variation of ultrasonic pulse velocity and amplitude in dry and wet states with temperature was studied. Then, the correlation between the ultrasonic parameters and both the volume parameters and the mechanical performance parameters of asphalt mixtures was revealed, and the functional relationship between ultrasonic pulse velocity and compressive strength was established. Finally, the reliability of predicting high-frequency dynamic modulus by ultrasonic velocity was verified. The laboratory tests and analysis results indicate that both ultrasonic pulse velocity and amplitude in dry and wet conditions show a decreasing trend with an increase in temperature. Ultrasonic parameters are greatly influenced by asphalt content and mineral aggregate content of 9.5~13.2 mm and 13.2~16 mm. The dynamic modulus at a high-frequency load can be predicted by using ultrasonic velocity, and predicting the results for OGFC and SMA mixtures deduced by using the UPV at a high-frequency load have higher reliability.
Transient Convective Heat Transfer in Porous Media
Authors: D’Rose Ruben , Willemsz Mark , Smeulders David
Published: October 2023

In this study, several methods to analyze convective heat transfer in a porous medium are presented and discussed. First, the method of Fourier was used to obtain solutions for reduced temperatures θs and θf. The results showed an exponentially decaying propagating temperature front. Then, we discuss the method of integration that was presented earlier by Schumann. This method makes use of a transformation of variables. Thirdly, the system of partial differential equations was directly solved with the Finite Difference method, of which the result showed good agreement with the Fourier solutions. For the chosen Δτ and Δξ, the maximum error for θf=3.7%. The maximum error for θs for the first ξ and first τ is large (36%) but decays rapidly. The problem was extended by adding a linear heat source term to the solid. Again, making use of the change in variables, analytical solutions were derived for the solid and fluid phases, and corrections to the previous literature were suggested. Finally, results obtained from a numerical model were compared to the analytical solutions, which again showed good agreement (maximum error of 6%).
Optimal Coherent Point Selection for 3D Quality Inspection from Silhouette-Based Reconstructions
Authors: Pérez Soler Javier , Guardiola Jose-Luis , Perez Jimenez Alberto , Garrigues Carbó Pau , García Sastre Nicolás , Perez-Cortes Juan-Carlos
Published: October 2023

3D Geometric quality inspection involves assessing and comparing a reconstructed object to a predefined reference model or design that defines its expected volume. Achieving precise 3D object geometry reconstruction from multiple views can be challenging. In this research, we propose a camera-coherent point selection method to measure differences with the reference. The result is a point cloud extracted from the reconstruction that represents the best-case scenario, ensuring that any deviations from the reference are represented as seen from the cameras. This algorithm has been tested in both simulated and real conditions, reducing reconstruction errors by up to one fifth compared to traditional 3D reconstruction methodologies. Furthermore, this strategy assures that any existing difference with its reference really exists and it is a best-case scenario. It offers a fast and robust pipeline for comprehensive 3D geometric quality assurance, contributing significantly to advancements in the field of 3D object inspection.
How to Determine the Optimal Number of Cardiologists in a Region?
Authors: Nagapetyan Artur , Drozd Alexander , Subbotovsky Dmitry
Published: October 2023

This paper proposes an approach to determining the optimal number of medical specialists in a particular territory. According to the author’s theoretical model, in order to maximise public welfare, the marginal contribution of the last physician recruited to the growth of the public utility function should be equal to the marginal cost of attracting them and providing conditions for their work. To empirically assess the contribution of physicians to the number of lives saved, the CVD mortality rate is modelled using the instrumental variable method. At the level of provision of cardiologists in the amount of 1 per 100,000 people, their marginal contribution to the number of lives saved is not less than 124 per 100,000 people, with a further decrease of 10 per 100,000 people with an increase in the level of provision of one unit. The use of the obtained results will increase the validity of managerial decisions and improve the determination of the optimal number of doctors when choosing between alternative possibilities of spending money on hiring doctors with different profiles or other expenses, especially in the case of limited resources.
Electrochemical Properties and Structure of Membranes from Perfluorinated Copolymers Modified with Nanodiamonds
Authors: Lebedev Vasily T. , Kulvelis Yuri V. , Shvidchenko Alexandr V. , Primachenko Oleg N. , Odinokov Alexei S. , Marinenko Elena A. , Kuklin Alexander I. , Ivankov Oleksandr I.
Published: October 2023

In this study, we aimed to design and research proton-conducting membranes based on Aquivion®-type material that had been modified with detonation nanodiamonds (particle size 4–5 nm, 0.25–5.0 wt. %). These nanodiamonds carried different functional groups (H, OH, COOH, F) that provided the hydrophilicity of the diamond surface with positive or negative potential, or that strengthened the hydrophobicity of the diamonds. These variations in diamond properties allowed us to find ways to improve the composite structure so as to achieve better ion conductivity. For this purpose, we prepared three series of membrane films by first casting solutions of perfluorinated Aquivion®-type copolymers with short side chains mixed with diamonds dispersed on solid substrates. Then, we removed the solvent and the membranes were structurally stabilized during thermal treatment and transformed into their final form with –SO3H ionic groups. We found that the diamonds with a hydrogen-saturated surface, with a positive charge in aqueous media, contributed to the increase in proton conductivity of membranes to a greater rate. Meanwhile, a more developed conducting diamond-copolymer interface was formed due to electrostatic attraction to the sulfonic acid groups of the copolymer than in the case of diamonds grafted with negatively charged carboxyls, similar to sulfonic groups of the copolymer. The modification of membranes with fluorinated diamonds led to a 5-fold decrease in the conductivity of the composite, even when only a fraction of diamonds of 1 wt. % were used, which was explained by the disruption in the connectivity of ion channels during the interaction of such diamonds mainly with fluorocarbon chains of the copolymer. We discussed the specifics of the mechanism of conductivity in composites with various diamonds in connection with structural data obtained in neutron scattering experiments on dry membranes, as well as ideas about the formation of cylindrical micelles with central ion channels and shells composed of hydrophobic copolymer chains. Finally, the characteristics of the network of ion channels in the composites were found depending on the type and amount of introduced diamonds, and correlations between the structure and conductivity of the membranes were established.
From Bauxite as a Critical Material to the Required Properties of Cast Aluminum Alloys for Use in Electro Automotive Parts
Authors: Djurdjevic Mile , Manasijevic Srecko , Mihailović Marija , Stopic Srecko
Published: October 2023

There is a long process to transform bauxite, a critical raw material, into a substance with the required properties of cast aluminum alloys for use in electro automotive parts. Thanks to its unique properties, aluminum has become the material of choice for clean technology manufacturers in applications such as use in the automotive industry, renewable energy, batteries, electrical systems, resource-saving packaging, energy efficient buildings and clean mobility. Restructuring of the economy, the oil crisis, air pollution and global warming are some of the factors that have moved the automotive industry towards electrification since the beginning of the 21st century. This paper aims to highlight the required properties of cast aluminum alloys applied to the production of electro automotive parts, such as their mechanical and thermophysical properties, dimensional stability, corrosion resistance, electromagnetic compatibility and crashworthiness. Furthermore, this paper discusses which of the cast aluminum–silicon alloys, as well as the heat treatments and casting processes, are most suitable.
Searching for Unknown Material Properties for AM Simulations
Authors: Flood Aaron , Boillat Rachel , Isanaka Sriram Praneeth , Liou Frank
Published: October 2023

Additive manufacturing (AM) simulations are effective for materials that are well characterized and published; however, for newer or proprietary materials, they cannot provide accurate results due to the lack of knowledge of the material properties. This work demonstrates the process of the application of mathematical search algorithms to develop an optimized material dataset which results in accurate simulations for the laser directed energy deposition (DED) process. This was performed by first using a well-characterized material, Ti-64, to show the error in the predicted melt pool was accurate, and the error was found to be less than two resolution steps. Then, for 7000-series aluminum using a generic material property dataset from sister alloys, the error was found to be over 600%. The Nelder–Mead search algorithm was then applied to the problem and was able to develop an optimized dataset that had a combined width and depth error of just 9.1%, demonstrating that it is possible to develop an optimized material property dataset that facilitates more accurate simulation of an under-characterized material.
Silicon–Organic Hybrid Electro-Optic Modulator and Microwave Photonics Signal Processing Applications
Authors: Zhou Zihan , Chao Meng , Su Xinxin , Fu Shuanglin , Liu Ruonan , Li Zhihua , Bo Shuhui , Chen Zhuo , Wu Zhenlin , Han Xiuyou
Published: October 2023

Electro-optic modulator (EOM) is one of the key devices of high-speed optical fiber communication systems and ultra-wideband microwave photonic systems. Silicon–organic hybrid (SOH) integration platform combines the advantages of silicon photonics and organic materials, providing a high electro-optic effect and compact structure for photonic integrated devices. In this paper, we present an SOH-integrated EOM with comprehensive investigation of EOM structure design, silicon waveguide fabrication with Slot structure, on-chip poling of organic electro-optic material, and characterization of EO modulation response. The SOH-integrated EOM is measured with 3 dB bandwidth of over 50 GHz and half-wave voltage length product of 0.26 V·cm. Furthermore, we demonstrate a microwave photonics phase shifter by using the fabricated SOH-integrated dual parallel Mach–Zehnder modulator. The phase shift range of 410° is completed from 8 GHz to 26 GHz with a power consumption of less than 38 mW.
Tribological Properties of Groove-Textured Ti-6Al-4V Alloys with Solid Lubricants in Dry Sliding against GCr15 Steel Balls
Authors: Wu Ze , Tan Xiuli , Li Guochao , Xing Youqiang
Published: October 2023

A nanosecond laser is used to fabricate groove-patterned textures on the upper surface of Ti-6Al-4V alloys, and then molybdic sulfide solid lubricants are filled into the grooves. The treated titanium alloy is subjected to friction and wear tests. The tribological performances of Ti-6Al-4V alloys are evaluated, and the wearing mechanism is analyzed. The combination of solid lubricants and surface texturing can effectively reduce the frictional coefficient and reduce the adhesion of Ti-6Al-4V materials on the steel balls for friction. The main wearing mechanism is the adhesive wear of the Ti-6Al-4V alloy and the adhesion of Ti-6Al-4V alloy materials on the surface of the steel balls. During the friction process, solid lubricants are squeezed from the grooves and coated at the friction interface to form a solid lubrication layer. This is the important reason why the combination of surface texturing and solid lubricants can improve the friction properties of titanium alloys effectively. The combination of solid lubricants and laser surface texturing provides an effective alternative way to improve the tribological properties of titanium alloy materials.
Machine Learning-Based Classification of Mango Pulp Weevil Activity Utilizing an Acoustic Sensor
Authors: Banlawe Ivane Ann P. , dela Cruz Jennifer C.
Published: October 2023

The mango pulp weevil (MPW) is an aggressive pest that mates seasonally according to the cycle of the mango fruit. After discovering the existence of the mango pulp weevil in Palawan, the island has been under quarantine for exporting mangoes. Detection of the pest proves difficult as the pest does not leave a physical sign that the mango has been damaged. Infested mangoes are wasted as they cannot be sold due to damage. This study serves as a base study for non-invasive mango pulp weevil detection using MATLAB machine learning and audio feature extraction tools. Acoustic sensors were evaluated for best-fit use in the study. The rationale for selecting the acoustic sensors includes local availability and accessibility. Among the three sensors tested, the MEMS sensor had the best result. The data for acoustic frequency are acquired using the selected sensor, which is placed inside a soundproof chamber to minimize the noise and isolate the sound produced by each activity. The identified activity of the adult mango pulp weevil includes walking, resting, and mating. The Mel-frequency cepstral coefficient (MFCC) was used for feature extraction of the recorded audio and training of the SVM classifier. The study achieved 89.81% overall accuracy in characterizing mango pulp weevil activity.
Quasi-3D Model for Lateral Resonances on Homogeneous BAW Resonators
Authors: Udaondo Carlos , Collado Carlos , Mateu Jordi
Published: October 2023

Lateral modes are responsible for the in-band spurious resonances that appear on BAW resonators, degrading the in-band filter response. In this work, a fast computational method based on the transmission line matrix (TLM) method is employed to model the lateral resonances of BAW resonators. Using the precomputed dispersion curves of Lamb waves and an equivalent characteristic impedance for the TE1 mode, a network of transmission lines is used to calculate the magnitude of field distributions on the electrodes. These characteristics are specific to the stack layer configuration. The model’s implementation is based on nodal Y matrices, from which particle displacement profiles are coupled to the electric domain via piezoelectric constitutive relations. Consequently, the input impedance of the resonator is obtained. The model exhibits strong agreement with FEM simulations of FBARs and SMRs, and with measurements of several SMRs. The proposed model can provide accurate predictions of resonator input impedance, which is around 200 times faster than conventional FEM.
Revisiting the Raman Spectra of Carbonate Minerals
Authors: Alves Julliana F. , Edwards Howell G. M. , Korsakov Andrey , de Oliveira Luiz Fernando C.
Published: October 2023

This work presents a new discussion about the vibrational properties of the carbonate ion displayed in several different environments. The microparameters introduced by cation substitution and different crystal lattices in addition to the crystal aggregation are present in the discussion. The work comments on how the Raman modes are affected by these changes by using data obtained with four different laser excitation sources. Raman spectra excited at 1064 nm are reported at 1 cm−1 resolution. New observations and approaches based on the Raman modes highlight the differences observed in the relative intensity and width of the bands. The new data contribute to the understanding of these materials and their spectra, bringing new observations based on the Raman modes. This work presents a new approach highlighting the differences observed in the relative intensity and width of the Raman bands. The results indicate some evidence of the influence of the crystal habit and/or the growth of the mineral itself on the Raman spectrum. In addition, the data show the influence of cation substitution upon Raman bandwidth and the interference of the size of the spot of the laser in the measurement.
The Addition of Co into CuO–ZnO Oxides Triggers High Antibacterial Activity and Low Cytotoxicity
Authors: Bauer Elvira Maria , Talone Alessandro , Imperatori Patrizia , Briancesco Rossella , Bonadonna Lucia , Carbone Marilena
Published: October 2023

In the present work, a simple two-step method is proposed for mixed oxide synthesis aimed at the achievement of antibacterial nanomaterials. In particular, Cu, Zn and Co have been selected to achieve single-, double- and triple-cation oxides. The synthesized samples are characterized by XRD, IR, SEM and EDX, indicating the formation of either crystalline or amorphous hydrocarbonate precursors. The oxides present one or two crystalline phases, depending on their composition; the triple-cation oxides form a solid solution of tenorite. Also, the morphology of the samples varies with the composition, yielding nanoparticles, filaments and hydrangea-like microaggregates. The antibacterial assays are conducted against E. coli and indicate an enhanced efficacy, especially displayed by the oxide containing 3% Co and 9% Zn incorporated into the CuO lattice. The oxides with the highest antibacterial properties are tested for their cytotoxicity, indicating a low toxicity impact, in line with literature data.
Biocompatibility Evaluation of TiO2, Fe3O4, and TiO2/Fe3O4 Nanomaterials: Insights into Potential Toxic Effects in Erythrocytes and HepG2 Cells
Authors: Paramo Luis , Jiménez-Chávez Arturo , Medina-Ramirez Iliana E. , Böhnel Harald Norbert , Escobar-Alarcón Luis , Esquivel Karen
Published: October 2023

Nanomaterials such as titanium dioxide and magnetite are increasingly used in several fields, such as water remediation and agriculture. However, this has raised environmental concerns due to potential exposure to organisms like humans. Nanomaterials can cause adverse interactions depending on physicochemical characteristics, like size, morphology, and composition, when interacting with living beings. To ensure safe use and prevent the risk of exposure to nanomaterials, their biocompatibility must be assessed. In vitro cell cultures are beneficial for assessing nanomaterial–cell interactions due to their easy handling. The present study evaluated the biocompatibility of TiO2, Fe3O4, and TiO2/Fe3O4 nanomaterials thermally treated at 350 °C and 450 °C in erythrocytes and HepG2 cells. According to the hemolysis experiments, non-thermally treated NMs are toxic (>5% hemolysis), but their thermally treated counterparts do not present toxicity (<2%). This behavior indicates that the toxicity derives from some precursor (solvent or surfactant) used in the synthesis of the nanomaterials. All the thermally treated nanomaterials did not show hemolytic activity under different conditions, such as low-light exposure or the absence of blood plasma proteins. In contrast, non-thermally treated nanomaterials showed a high hemolytic behavior, which was reduced after the purification (washing and thermal treatment) of nanomaterials, indicating the presence of surfactant residue used during synthesis. An MTS cell viability assay shows that calcined nanomaterials do not reduce cell viability (>11%) during 24 h of exposure. On the other hand, a lactate dehydrogenase leakage assay resulted in a higher variability, indicating that several nanomaterials did not cause an increase in cell death as compared to the control. However, a holotomographic microscopy analysis reveals a high accumulation of nanomaterials in the cell structure at a low concentration (10 µg mL−1), altering cell morphology, which could lead to cell membrane damage and cell viability reduction.
State-of-the-Art 2D and Carbon Nanomaterials in France
Authors: Journet Catherine
Published: October 2023

Nanotechnology has revolutionized various industries by enabling the manipulation and fabrication of materials at the nanoscale [...]
A Giant Exchange Bias Effect Due to Enhanced Ferromagnetism Using a Mixed Martensitic Phase in Ni50Mn37Ga13 Spun Ribbons
Authors: Tian Fanghua , Zhao Qizhong , Guo Jiale , Kong Sen , Liu Bingjie , Dai Zhiyong , Fang Minxia , Zhang Yin , Zhou Chao , Cao Kaiyan , Yang Sen
Published: October 2023

The structure of a material is an important factor in determining its physical properties. Here, we adjust the structure of the Ni50Mn37Ga13 spun ribbons by changing the wheel speed to regulate the exchange bias effect of the material. The characterization results of micromorphology and structure show that as the wheel speed increases, the martensite lath decreases from 200 nm to 50 nm, the structure changed from the NM to a NM and 10M mixed martensitic structure containing mainly NM, then changed to NM and 10M where 10M and NM are approaching. Meanwhile, HE first increased and then decreased as the wheel speed increased. The optimum exchange bias effect (HE = 7.2 kOe) occurs when the wheel speed is 25 m∙s−1, mainly attributed to the enhanced ferromagnetism caused by part of 10M in NM martensite, which enhanced the exchange coupling of ferromagnetism and antiferromagnetism. This work reveals the structural dependence of exchange bias and provides a way to tune the magnitude of the exchange bias of Heusler alloys.
Long-Term Consumption of Green Tea Can Reduce the Degree of Depression in Postmenopausal Women by Increasing Estradiol
Authors: Wan Zhenyu , Qin Xucong , Tian Yuling , Ouyang Fangcheng , Wang Gaohua , Wan Qirong
Published: October 2023

Postmenopausal women face a higher risk of depression due to a combination of social and physiological factors. As a beverage rich in a variety of bioactive substances, green tea has significant effects on metabolism, inflammation and endocrine, and may reduce the risk of depression, but few studies have looked at the effects of green tea on postmenopausal women. Therefore, we designed this study to investigate the effects of long-term green tea consumption on inflammation, endocrine and depression levels in postmenopausal women. We investigated a tea-producing village and eventually included 386 postmenopausal women, both in the tea drinking and control groups. The results showed that there were significant differences in the degree of insomnia, degree of depression, BMI, SII and estradiol between the two groups. And, green tea consumption may reduce the risk of depression through the mediating pathway of sleep, SII and estradiol. In summary, long-term green tea consumption can reduce the risk of depression in postmenopausal women by reducing inflammation and increasing estradiol. This kind of living habit deserves further promotion.
The Mediterranean Diet: Effects on Insulin Resistance and Secretion in Individuals with Overweight or Obesity
Authors: Vetrani Claudia , Verde Ludovica , Colao Annamaria , Barrea Luigi , Muscogiuri Giovanna
Published: October 2023

High adherence to the Mediterranean Diet (MD) is associated with a lower risk of type 2 diabetes. However, it is less clear whether the different MD food items might influence specific biological functions related to glucose tolerance, i.e., insulin resistance (IR) and/or secretion (IS). Thus, this cross-sectional study aimed to investigate the relationship between adherence to MD and IR, insulin sensitivity, and IS in individuals with overweight/obesity. Participants (62 individuals; 7M/55F; mean age 49 ± 15 years; mean BMI 35.8 ± 6.7 kg/m²) underwent a 75 g oral glucose tolerance test (OGTT) to assess plasma glucose and insulin concentrations. These parameters were used for the calculation of validated IR indices (Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), Homeostatic Model Assessment for β-cell function (HOMA-β)), as well as insulin sensitivity indices (insulin sensitivity index (ISI), oral glucose insulin sensitivity (OGIS)). MD adherence was gauged using the PREDIMED questionnaire. Bivariate correlations were used to highlight the association between OGTT-derived indices and MD adherence (PREDIMED score) or specific foodstuffs related to MD. Despite there being no significant differences in BMI, impaired fasting glucose (IFG), and impaired glucose tolerance (IGT), the high MD adherence group presented lower HOMA-IR (p = 0.022) and higher ISI (p = 0.033) compared to other groups. High MD adherence was inversely correlated with HOMA-IR (r = −0.400; p = 0.004) and directly correlated with ISI (r = 0.296, p = 0.039). Fish consumption, a key component of MD, exhibited significant associations: it was directly correlated to ISI (r = 0.394, p = 0.005) and inversely related to HOMA-IR (r = −0.327, p = 0.019) and β-cell function (r = −0.489, p < 0.001). In conclusion, a high MD adherence, and in particular the consumption of fish, is associated with a decreased IR in individuals with overweight/obesity.
The Association among Urinary Lead and Cadmium, Serum Adiponectin, and Serum Apoptotic Microparticles in a Young Taiwanese Population
Authors: Lin Chien-Yu , Wang Chi-Kang , Sung Fung-Chang , Su Ta-Chen
Published: October 2023

Previous studies reported that lead (Pb) and cadmium (Cd) exposure are linked to changes in serum adiponectin; an adipokine that promotes glycolysis and inhibits gluconeogenesis to regulate glucose metabolism. However, no study has ever explored the relationship between exposure to these two heavy metals and adiponectin in adolescents and young adults. Additionally, the role of adiponectin in the relationship between Pb and Cd exposure and vascular endothelial cell apoptosis has never been investigated. In this study, 724 Taiwanese participants, aged 12 to 30 years, were enrolled to investigate the association among urinary lead and cadmium, serum adiponectin, and apoptotic microparticles (CD31+/CD42a−, CD31+/CD42a+, and CD14). The results of the current study revealed a statistically significant inverse association between urine Pb and Cd levels and adiponectin levels, as well as a positive association with apoptotic microparticles (CD31+/CD42a−, CD31+/CD42a+, and CD14). Adiponectin was also inversely correlated with CD31+/CD42a− and CD31+/CD42a+. Moreover, when subjects with both Pb and Cd levels above the 50th percentile were compared to those below it, the former group exhibited the lowest average adiponectin value. Additionally, a more pronounced positive association between heavy metals and apoptotic microparticles (CD31+/CD42a− and CD31+/CD42a+) was observed when adiponectin levels were lower. Furthermore, an interaction between adiponectin and heavy metals was identified in the relationship between these metals and CD31+/CD42a−. In conclusion, these findings suggest that Pb and Cd exposure may have an adverse effect on adiponectin, and it may play a role in the link between heavy metal exposure and the dysfunction of vascular endothelial cells. Future studies are needed to establish whether a causal relationship exists.
Protein Disulfide Isomerase Family A Member 3 Knockout Abrogate Effects of Vitamin D on Cellular Respiration and Glycolysis in Squamous Cell Carcinoma
Authors: Nowak Joanna I. , Olszewska Anna M. , Król Oliwia , Żmijewski Michał A.
Published: October 2023

PDIA3 is an endoplasmic reticulum disulfide isomerase, which is involved in the folding and trafficking of newly synthesized proteins. PDIA3 was also described as an alternative receptor for the active form of vitamin D (1,25(OH)2D3). Here, we investigated an impact of PDIA3 in mitochondrial morphology and bioenergetics in squamous cell carcinoma line A431 treated with 1,25(OH)2D3. It was observed that PDIA3 deletion resulted in changes in the morphology of mitochondria including a decrease in the percentage of mitochondrial section area, maximal diameter, and perimeter. The 1,25(OH)2D3 treatment of A431∆PDIA3 cells partially reversed the effect of PDIA3 deletion increasing aforementioned parameters; meanwhile, in A431WT cells, only an increase in mitochondrial section area was observed. Moreover, PDIA3 knockout affected mitochondrial bioenergetics and modulated STAT3 signaling. Oxygen consumption rate (OCR) was significantly increased, with no visible effect of 1,25(OH)2D3 treatment in A431∆PDIA3 cells. In the case of Extracellular Acidification Rate (ECAR), an increase was observed for glycolysis and glycolytic capacity parameters in the case of non-treated A431WT cells versus A431∆PDIA3 cells. The 1,25(OH)2D3 treatment had no significant effect on glycolytic parameters. Taken together, the presented results suggest that PDIA3 is strongly involved in the regulation of mitochondrial bioenergetics in cancerous cells and modulation of its response to 1,25(OH)2D3, possibly through STAT3.
Media Exposure of Suicidal Behaviour: An Umbrella Review
Authors: Sufrate-Sorzano Teresa , Di Nitto Marco , Garrote-Cámara María Elena , Molina-Luque Fidel , Recio-Rodríguez José Ignacio , Asión-Polo Pilar , Durante Ángela , Gea-Caballero Vicente , Juárez-Vela Raúl , Pérez Jesús , Santolalla-Arnedo Iván
Published: October 2023

Aim: To analyse recommended interventions for the safe and responsible dissemination of suicidal behaviour in the media for preventive purposes. Background: Suicide is a serious public health problem that leads to more than 700,000 deaths per year, which translates into one death every forty seconds. The media play a significant role in shaping public perceptions and reflecting societal issues. Because of its active role in the construction of reality, the way in which the media report and expose suicidal behaviour has the capacity to influence the population in either a preventive or harmful way. Design: An umbrella review was carried out and a report was written according to the Preferred Reporting Items for Overviews of Reviews. Methods: We systematically searched for reviews published from inception to February 2023 in MEDLINE (PubMed), CINAHL and PsycInfo (via EBSCOhost), Web of Science, Embase, Cochrane Library of Systematic Reviews, Scopus, and Google Scholar. A narrative synthesis of the results was conducted. Results: Six systematic reviews with a moderate to high quality level were selected. Among the recommended interventions were the inclusion of positive messages of hope, resilience, or of overcoming the event, narratives with information on available resources or the promotion of support-seeking attitudes as an effective prevention mechanism, as well as the avoidance of repetitive reporting of the same suicide. The appropriate and responsible dissemination of information on suicidal behaviour in the media with complete and up-to-date information on available centres, organisations, institutions, and resources has proven to be effective, especially in vulnerable populations. Conclusion: Educating and training the media in an appropriate approach to disseminating suicidal behaviour helps to reduce the number of suicidal behaviours. Knowing what information is advisable to include in the news item as well as what information to avoid is a strong point. Guidelines to promote responsible media reporting are a key component of suicide prevention strategies. This study was prospectively registered in the International Prospective Register of Systematic Reviews (PROSPERO) on 23 April 2022 with the registration number CRD42022320393.
Genetic Evaluation of Bovine Papillomavirus Types Associated with Teat Papillomatosis in Polish Dairy Cattle with the Report of a New Putative Type
Authors: Pyrek Paulina , Bednarski Michał , Popiel Jarosław , Siedlecka Magdalena , Karwańska Magdalena
Published: October 2023

Teat papillomatosis is reported to be one of the factors causing mastitis and milk losses in dairy cattle. Little is known about bovine papillomavirus (BPV) circulation in the European cattle population, and no reports can yet be found about its prevalence in Polish herds. In this study, 177 BPV-like lesions were collected from teats of 109 slaughtered cows. BPV was identified in 39 of the examined animals, using PCR amplification and Sanger dideoxy sequencing. In total, 10 BPV types were isolated, among which the most common were infections caused by types 8 and 7. Macroscopically, “rice-grain” type lesions dominated (76%) and were mainly found on one teat (57.4%). The diversity of BPV types causing teat papillomatosis in Polish cows seems to be large, with nine already known types isolated and a new putative type found. The spread of new types among the worldwide cattle population can be seen for the first time, as type 25 and so called isolates BPV42 and BPV43 were found in the European cattle population.
Natural Products for the Potential Use of Neuroprotective and Neurorestorative Effects in Stroke
Authors: Chen Hansen , Liu Qingkun
Published: October 2023

Stroke is the second leading cause of death and the third leading cause of disability worldwide, with limited treatment options [...]
Preparation of Nanosized Pharmaceutical Formulations by Dual Centrifugation
Authors: Koehler Jonas K. , Schmager Stefanie , Bender Valentin , Steiner Denise , Massing Ulrich
Published: October 2023

Dual centrifugation (DC) is an innovative in-vial homogenization and in-vial nanomilling technique that has been in use for the preparation of liposomes for more than one decade. Since then, DC has continuously been developed for preparing various liposomes and other lipid nanoparticles including emulsions and solid lipid nanoparticles (SLNs) as well as polymersomes and nanocrystals. Improvements in equipment technology have been achieved over the past decade, so that DC is now on its way to becoming the quasi-standard for the simple, fast, and aseptic production of lipid nanoparticles and nanocrystals in small and medium batch sizes, including the possibility of simple and fast formulation screening or bedside preparations of therapeutic nanoparticles. More than 68 publications in which DC was used to produce nanoparticles have appeared since then, justifying an initial review of the use of DC for pharmaceutical nanotechnology.
Changes in Cardiovascular and Renal Biomarkers Associated with SGLT2 Inhibitors Treatment in Patients with Type 2 Diabetes Mellitus
Authors: Peppa Melpomeni , Manta Aspasia , Mavroeidi Ioanna , Asimakopoulou Athina , Syrigos Alexandros , Nastos Constantinos , Pikoulis Emmanouil , Kollias Anastasios
Published: October 2023

Type 2 diabetes mellitus is a major health problem worldwide with a steadily increasing prevalence reaching epidemic proportions. The major concern is the increased morbidity and mortality due to diabetic complications. Traditional but also nontraditional risk factors have been proposed to explain the pathogenesis of type 2 diabetes mellitus and its complications. Hyperglycemia has been considered an important risk factor, and the strict glycemic control can have a positive impact on microangiopathy but not macroangiopathy and its related morbidity and mortality. Thus, the therapeutic algorithm has shifted focus from a glucose-centered approach to a strategy that now emphasizes target-organ protection. Sodium-glucose transporter 2 inhibitors is an extremely important class of antidiabetic medications that, in addition to their glucose lowering effect, also exhibit cardio- and renoprotective effects. Various established and novel biomarkers have been described, reflecting kidney and cardiovascular function. In this review, we investigated the changes in established but also novel biomarkers of kidney, heart and vascular function associated with sodium-glucose transporter 2 inhibitors treatment in patients with type 2 diabetes mellitus.
Chemical Composition and Insulin-Resistance Activity of Arginine-Loaded American Cranberry (Vaccinium macrocarpon Aiton, Ericaceae) Leaf Extracts
Authors: Koshovyi Oleh , Vlasova Inna , Laur Heleriin , Kravchenko Ganna , Krasilnikova Oksana , Granica Sebastian , Piwowarski Jakub P. , Heinämäki Jyrki , Raal Ain
Published: October 2023

One of the key pathogenetic links in type 2 diabetes mellitus (T2DM) is the formation of insulin resistance (IR). Besides a wide selection of synthetic antidiabetic drugs, various plant-origin extracts are also available to support the treatment of T2DM. This study aimed to investigate and gain knowledge of the chemical composition and potential IR correction effect of American cranberry (Vaccinium macrocarpon Aiton) leaf extracts and formulate novel 3D-printed oral dosage forms for such extracts. The bioactivity and IR of L-arginine-loaded cranberry leaf extracts were studied in vivo in rats. The cranberry leaf extracts consisted of quinic, 3-caffeoylquinic (chlorogenic), p-coumaroylquinic acids, quercetin 3-O-galactoside, quercetin-3-O-glucoside, quercetin-3-xyloside, quercetin-3-O-arabino pyranoside, quercetin-3-O-arabinofuranoside, quercetin 3-O-rhamnoside, and quercetin-O-p-coumaroyl hexoside-2 identified by HPLC. In vivo studies with rats showed that the oral administration of the cranberry leaf extracts had a positive effect on insulin sensitivity coefficients under the insulin tolerance test and affected homeostasis model assessment IR levels and liver lipid content with experimental IR. A novel 3D-printed immediate-release dosage form was developed for the oral administration of cranberry leaf extracts using polyethylene oxide as a carrier gel in semi-solid extrusion 3D printing. In conclusion, American cranberry leaf extracts loaded with L-arginine could find uses in preventing health issues associated with IR.
Electrospun Scaffolds of Polylactic Acid, Collagen, and Amorphous Calcium Phosphate for Bone Repair
Authors: Cárdenas-Aguazaco William , Camacho Bernardo , Gómez-Pachón Edwin , Lara-Bertrand Adriana , Silva-Cote Ingrid
Published: October 2023

Most electrospun scaffolds for bone tissue engineering typically use hydroxyapatite (HA) or beta tricalcium phosphate (β-TCP). However, the biological activity of these crystalline compounds can be limited due to their low solubility. Therefore, amorphous calcium phosphate (ACP) may be an alternative in bone repair scaffolds. This study analyzes the morphology, porosity, mechanical strength, and surface chemistry of electrospun scaffolds composed of polylactic acid and collagen integrated with hydroxyapatite (MHAP) or amorphous calcium phosphate (MACP). In addition, the in vitro biocompatibility, osteogenic differentiation, and growth factor production associated with bone repair using human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) are evaluated. The results show that the electrospun MHAP and MACP scaffolds exhibit a fibrous morphology with interconnected pores. Both scaffolds exhibit favorable biocompatibility and stimulate the proliferation and osteogenesis of hWJ-MSCs. However, cell adhesion and osteocalcin production are greater in the MACP scaffold compared to the MHAP scaffold. In addition, the MACP scaffold shows significant production of bone-repair-related growth factors such as transforming growth factor-beta 1 (TGF-β1), providing a solid basis for its use in bone tissue engineering.
Design, Development, Evaluation, and In Vivo Performance of Buccal Films Embedded with Paliperidone-Loaded Nanostructured Lipid Carriers
Authors: AlMulhim Fahad Mohammed , Nair Anroop B. , Aldhubiab Bandar , Shah Hiral , Shah Jigar , Mewada Vivek , Sreeharsha Nagaraja , Jacob Shery
Published: October 2023

The therapeutic effectiveness of paliperidone in the treatment of schizophrenia has been limited by its poor oral bioavailability; hence, an alternative route could be appropriate. This study investigates the feasibility of developing a buccal film impregnated with paliperidone-loaded nanostructured lipid carriers (NLCs) and assesses the potential to enhance its bioavailability. Box–Behnken-based design optimization of NLCs was performed by examining the particles’ physical characteristics. The polymeric film was used to load optimized NLCs, which were then assessed for their pharmaceutical properties, permeability, and pharmacokinetics. The optimization outcomes indicated that selected formulation variables had a considerable (p < 0.05) impact on responses such as particle size, entrapment efficiency, and % drug release. Desired characteristics such as a negative charge, higher entrapment efficiency, and nanoparticles with ideal size distribution were shown by optimized NLC dispersions. The developed film demonstrated excellent physico-mechanical properties, appropriate texture, good drug excipient compatibility (chemically stable formulation), and amorphous drug nature. A sustained Weibull model drug release (p < 0.0005) and superior flux (~5-fold higher, p < 0.005) were seen in NLC-loaded film compared to plain-drug-loaded film. The pharmacokinetics profile in rabbits supports the goal of buccal therapy as evidenced by significantly higher AUC0–12 (p < 0.0001) and greater relative bioavailability (236%) than the control. These results support the conclusion that paliperidone-loaded NLC buccal film has the potential to be an alternate therapy for its effective administration in the treatment of schizophrenia.
Development and Evaluation of a Self-Nanoemulsifying Drug Delivery System for Sinapic Acid with Improved Antiviral Efficacy against SARS-CoV-2
Authors: Alhadrami Hani A. , El-Din Ahmed S.G. Srag , Hassan Hossam M. , Sayed Ahmed M. , Alhadrami Albaraa H. , Rateb Mostafa E. , Naguib Demiana M.
Published: October 2023

This study aimed to develop a self-nanoemulsifying drug delivery system (SNE) for sinapic acid (SA) to improve its solubility and antiviral activity. Optimal components for the SA-SNE formulation were selected, including Labrafil as the oil, Cremophor EL as the surfactant, and Transcutol as the co-surfactant. The formulation was optimized using surface response design, and the optimized SA-SNE formulation exhibited a small globule size of 83.6 nm, high solubility up to 127.1 ± 3.3, and a 100% transmittance. In vitro release studies demonstrated rapid and high SA release from the formulation. Pharmacokinetic analysis showed improved bioavailability by 2.43 times, and the optimized SA-SNE formulation exhibited potent antiviral activity against SARS-CoV-2. The developed SA-SNE formulation can enhance SA’s therapeutic efficacy by improving its solubility, bioavailability, and antiviral activity. Further in silico, modeling, and Gaussian accelerated molecular dynamics (GaMD)-based studies revealed that SA could interact with and inhibit the viral main protease (Mpro). This research contributes to developing effective drug delivery systems for poorly soluble drugs like SA, opening new possibilities for their application via nebulization in SARS-CoV-2 therapy.
The Casimir Force between Two Graphene Sheets: 2D Fresnel Reflection Coefficients, Contributions of Different Polarizations, and the Role of Evanescent Waves
Authors: Klimchitskaya Galina L. , Mostepanenko Vladimir M.
Published: October 2023

We consider the Casimir pressure between two graphene sheets and contributions to it determined by evanescent and propagating waves with different polarizations. For this purpose, the derivation of the 2-dimensional (2D) Fresnel reflection coefficients on a graphene sheet is presented in terms of the transverse and longitudinal dielectric permittivities of graphene with due account of the spatial dispersion. The explicit expressions for both dielectric permittivities as the functions of the 2D wave vector, frequency, and temperature are written along the real frequency axis in the regions of propagating and evanescent waves and at the pure imaginary Matsubara frequencies using the polarization tensor of graphene. It is shown that in the application region of the Dirac model nearly the total value of the Casimir pressure between two graphene sheets is determined by the electromagnetic field with transverse magnetic (TM) polarization. By using the Lifshitz formula written along the real frequency axis, the contributions of the TM-polarized propagating and evanescent waves into the total pressure are determined. By confronting these results with the analogous results found for plates made of real metals, the way for bringing the Lifshitz theory using the realistic response functions in agreement with measurements of the Casimir force between metallic test bodies is pointed out.
Anti-Biofilm Activity of Carnosic Acid from Salvia rosmarinus against Methicillin-Resistant Staphylococcus aureus
Authors: Iobbi Valeria , Parisi Valentina , Bernabè Giulia , De Tommasi Nunziatina , Bisio Angela , Brun Paola
Published: October 2023

The Salvia rosmarinus “Eretto Liguria” ecotype was studied as a source of valuable bioactive compounds. LC-MS analysis of the methanolic extract underlined the presence of diterpenoids, triterpenoids, polyphenolic acids, and flavonoids. The anti-virulence activity of carnosic acid along with the other most abundant compounds against methicillin-resistant Staphylococcus aureus (MRSA) was evaluated. Only carnosic acid induced a significant reduction in the expression of agrA and rnaIII genes, which encode the key components of quorum sensing (QS), an intracellular signaling mechanism controlling the virulence of MRSA. At a concentration of 0.05 mg/mL, carnosic acid inhibited biofilm formation by MRSA and the expression of genes involved in toxin production and made MRSA more susceptible to intracellular killing, with no toxic effects on eukaryotic cells. Carnosic acid did not affect biofilm formation by Pseudomonas aeruginosa, a human pathogen that often coexists with MRSA in complex infections. The selected ecotype showed a carnosic acid content of 94.3 ± 4.3 mg/g. In silico analysis highlighted that carnosic acid potentially interacts with the S. aureus AgrA response regulator. Our findings suggest that carnosic acid could be an anti-virulence agent against MRSA infections endowed with a species-specific activity useful in multi-microbial infections.