bluuebunny/crossref_metadata_2025 huggingface dataset:
MicroRNA-16 Restores Sensitivity to Tyrosine Kinase Inhibitors and Outperforms MEK Inhibitors in KRAS-Mutated Non-Small Cell Lung Cancer
Authors: Fanini Francesca , Bandini Erika , Plousiou Meropi , Carloni Silvia , Wise Petra , Neviani Paolo , Murtadha Mariam , Foca Flavia , Fabbri Francesco , Vannini Ivan , Fabbri Muller
Published: December 2021

Background: Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. Chemotherapy, the treatment of choice in non-operable cases, achieves a dismal success rate, raising the need for new therapeutic options. In about 25% of NSCLC, the activating mutations of the KRAS oncogene define a subclass that cannot benefit from tyrosine kinase inhibitors (TKIs). The tumor suppressor miR-16 is downregulated in many human cancers, including NSCLC. The main objectives of this study were to evaluate miR-16 treatment to restore the TKI sensitivity and compare its efficacy to MEK inhibitors in KRAS-mutated NSCLC. Methods: We performed in vitro and in vivo studies to investigate whether miR-16 could be exploited to overcome TKI resistance in KRAS-mutated NSCLC. We had three goals: first, to identify the KRAS downstream effectors targeted by mir-16, second, to study the effects of miR-16 restoration on TKI resistance in KRAS-mutated NSCLC both in vitro and in vivo, and finally, to compare miR-16 and the MEK inhibitor selumetinib in reducing KRAS-mutated NSCLC growth in vitro and in vivo. Results: We demonstrated that miR-16 directly targets the three KRAS downstream effectors MAPK3, MAP2K1, and CRAF in NSCLC, restoring the sensitivity to erlotinib in KRAS-mutated NSCLC both in vitro and in vivo. We also provided evidence that the miR-16–erlotinib regimen is more effective than the selumetinib–erlotinib combination in KRAS-mutated NSCLC. Conclusions: Our findings support the biological preclinical rationale for using miR-16 in combination with erlotinib in the treatment of NSCLC with KRAS-activating mutations.
Activation of Innate Immunity by Therapeutic Nucleic Acids
Authors: Bishani Ali , Chernolovskaya Elena L.
Published: December 2021

Nucleic acid-based therapeutics have gained increased attention during recent decades because of their wide range of application prospects. Immunostimulatory nucleic acids represent a promising class of potential drugs for the treatment of tumoral and viral diseases due to their low toxicity and stimulation of the body’s own innate immunity by acting on the natural mechanisms of its activation. The repertoire of nucleic acids that directly interact with the components of the immune system is expanding with the improvement of both analytical methods and methods for the synthesis of nucleic acids and their derivatives. Despite the obvious progress in this area, the problem of delivering therapeutic acids to target cells as well as the unresolved issue of achieving a specific therapeutic effect based on activating the mechanism of interferon and anti-inflammatory cytokine synthesis. Minimizing the undesirable effects of excessive secretion of inflammatory cytokines remains an unsolved task. This review examines recent data on the types of immunostimulatory nucleic acids, the receptors interacting with them, and the mechanisms of immunity activation under the action of these molecules. Finally, data on immunostimulatory nucleic acids in ongoing and completed clinical trials will be summarized.
Effects of Menadione on Survival, Feeding, and Tunneling Activity of the Formosan Subterranean Termite
Authors: Ngo Kieu , Castillo Paula , Laine Roger A. , Sun Qian
Published: December 2021

The Formosan subterranean termite, Coptotermes formosanus Shiraki, is a highly destructive pest and a cosmopolitan invasive species. Sustainable termite management methods have been improving with the search for novel insecticides that are effective, safe, and cost efficient. Menadione, also known as vitamin K3, is a synthetic analogue and biosynthetic precursor of vitamin K with low mammalian toxicity. Menadione has shown insecticidal activity in several insects, presumably due to interference with mitochondrial oxidative phosphorylation. However, little is known about its effectiveness against termites. In this study, we evaluated the toxicity and repellency of menadione in C. formosanus. Our results showed that menadione affected the survival and feeding activity of termites both in filter paper and substrate (sand) treatments, and menadione influenced termite tunneling activity in treated sand. In a no-choice assay, ≥90% mortality after seven days and minimal or no food consumption were recorded when sand was treated with menadione at 6 to 600 ppm. In a two-choice assay with a combination of treated and untreated sand, termites were deterred by menadione at 6 to 600 ppm and exhibited low mortality (≤30%) over seven days, while tunneling activity was prevented with 60 to 600 ppm of menadione treatment. Overall, our study demonstrated dose-dependent toxicity and repellency of menadione in C. formosanus. The potential use of menadione as an alternative termite control agent is discussed.
The Myosin Myocardial Mesh Interpreted as a Biological Analogous of Nematic Chiral Liquid Crystals
Authors: Jouk Pierre-Simon , Usson Yves
Published: December 2021

There are still grey areas in the understanding of the myoarchitecture of the ventricular mass. This is despite the progress of investigation methods since the beginning of the 21st century (diffusion tensor magnetic resonance imaging, microcomputed tomography, and polarised light imaging). The objective of this article is to highlight the specificities and the limitations of polarised light imaging (PLI) of the unstained myocardium embedded in methyl methacrylate (MMA). Thus, to better differentiate our method from other PLI modes, we will refer to it by the acronym PLI-MMA. PLI-MMA shows that the myosin mesh of the compact left ventricular wall behaves like a biological analogous of a nematic chiral liquid crystal. Results obtained by PLI-MMA are: the main direction of the myosin molecules contained in an imaged voxel, the crystal liquid director n, and a regional isotropy index RI that is an orientation tensor, the equivalent of the crystal liquid order parameter. The vector n is collinear with the first eigenvector of diffusion tensor imaging (DTI-MRI). The RI has not been confounded with the diffusion tensor of DTI that gives information about the three eigenvectors of the ellipsoid of diffusion. PLI-MMA gives no information about the collagen network. The physics of soft matter has allowed the revisiting of Streeter’s conjecture on the myoarchitecture of the compact left ventricular wall: “geodesics on a nested set of toroidal surfaces”. Once the torus topology is understood, this characterisation of the myoarchitecture is more accurate and parsimonious than former descriptions. Finally, this article aims to be an enthusiastic invitation to a transdisciplinary approach between physicists of liquid crystals, anatomists, and specialists of imaging.
LPA Genotypes and Haplotypes Are Associated with Lipoprotein(a) Levels but Not Arterial Wall Properties in Stable Post-Coronary Event Patients with Very High Lipoprotein(a) Levels
Authors: Rehberger Likozar Andreja , Blinc Aleš , Trebušak Podkrajšek Katarina , Šebeštjen Miran
Published: December 2021

Lipoprotein(a) [Lp(a)] levels are an independent risk factor for coronary artery disease (CAD). Two single-nucleotide polymorphisms (rs10455872, rs3798220) and number of KIV-2 repeats in the gene encoding Lp(a) (LPA) are associated with Lp(a) and CAD. Our aim was to investigate whether in patients with stable CAD and high Lp(a) levels these genetic variants are associated with increased Lp(a) and arterial wall properties. Blood samples underwent biochemical and genetic analyses. Ultrasound measurements for the functional and morphological properties of arterial wall were performed. Genotypes of rs10455872 and haplotypes AT and GT showed significant association with Lp(a) levels. Patients with GG showed significantly higher Lp(a) levels compared with those with AG genotype (2180 vs. 1391 mg/L, p = 0.045). Patients with no AT haplotype had significantly higher Lp(a) compared to carriers of one AT haplotype (2158 vs. 1478 mg/L, p = 0.023) or two AT haplotypes (2158 vs. 1487 mg/L, p = 0.044). There were no significant associations with the properties of the arterial wall. Lp(a) levels significantly correlated also with number of KIV-2 repeats (r = −0.601; p < 0.0001). In our patients, these two LPA polymorphisms and number of KIV-2 repeats are associated with Lp(a), but not arterial wall properties.
Immunomonitoring of Monocyte and Neutrophil Function in Critically Ill Patients: From Sepsis and/or Trauma to COVID-19
Authors: Udovicic Ivo , Stanojevic Ivan , Djordjevic Dragan , Zeba Snjezana , Rondovic Goran , Abazovic Tanja , Lazic Srdjan , Vojvodic Danilo , To Kendrick , Abazovic Dzihan , Khan Wasim , Surbatovic Maja
Published: December 2021

Immune cells and mediators play a crucial role in the critical care setting but are understudied. This review explores the concept of sepsis and/or injury-induced immunosuppression and immuno-inflammatory response in COVID-19 and reiterates the need for more accurate functional immunomonitoring of monocyte and neutrophil function in these critically ill patients. in addition, the feasibility of circulating and cell-surface immune biomarkers as predictors of infection and/or outcome in critically ill patients is explored. It is clear that, for critically ill, one size does not fit all and that immune phenotyping of critically ill patients may allow the development of a more personalized approach with tailored immunotherapy for the specific patient. In addition, at this point in time, caution is advised regarding the quality of evidence of some COVID-19 studies in the literature.
Microplastics in Combined Sewer Overflows: An Experimental Study
Authors: Di Nunno Fabio , Granata Francesco , Parrino Francesco , Gargano Rudy , de Marinis Giovanni
Published: December 2021

One of the main sources of microplastics inside surface waters is represented by combined sewer overflows (CSOs), involving severe risks for the environment. The entry of microplastics into water bodies also depends on the characteristics of sewer diversion structures used as flow control devices. In this work, an experimental investigation was carried out to evaluate the outflow of microplastic particles, consisting of different types of nylon fibers, from a side weir located on a channel with a rectangular section. A specific methodology was developed for the fiber sampling and outflow assessment after the tests were performed. For the tested configurations, an increase in fibers discharged up to 196.15% was measured as the water flow rate increased by 62.75%, combined with an increase in the side weir length up to 40% and a decrease in the crest height up to 20%. The size and weight of the different fibers showed a low impact due to their low inertia, and their motion was governed by the water flow. An empirical equation to evaluate the fiber outflow as a function of water flow rate and side weir geometric characteristics was also proposed and calibrated for the experimentally tested ranges of the dimensionless lateral water outflow Q* = 0.51–0.83 and of the dimensionless geometric parameter S* = 0.114–0.200. These first experimental results make it possible to carry out a preliminary assessment of the impact of CSOs in terms of microplastics spilled into water bodies.
Application of the IMO Second Generation Intact Stability Criteria to a Ballast-Free Containership
Authors: Petacco Nicola , Pitardi Danilo , Podenzana Bonvino Carlo , Gualeni Paola
Published: December 2021

A methodology is presented to systematically modify the hull shape of a ballast-free container ship, in order to manage the issue of righting lever variation in waves. The IMO second generation intact stability criteria have been identified as a stability performance assessment tool, while the vertical prismatic coefficient has been selected as the leading parameter of hull modifications to carry out the sensitivity analysis. A revised Lackenby procedure has been chosen to make systematic changes at the hull form. The outcomes of this investigation point out that the proposed procedure is suitable to enable the ship to be fully compliant with the IMO vulnerability levels with minor design adjustment.
Predictive Course Control and Guidance of Autonomous Unmanned Sailboat Based on Efficient Sampled Gaussian Process
Authors: Dong Yuqin , Wu Nailong , Qi Jie , Chen Xinyuan , Hua Chenhua
Published: December 2021

In view of the vulnerability of ocean unmanned sailboats to the large lateral velocities due to wind and waves during navigation, this paper proposes a Gaussian Process Model Predictive Control (GPMPC) method based on data-driven learning technique to improve the navigation tracking accuracy of unmanned sailboats. The feature model of the sailing course change subject to the wind and waves is learned from the efficient sampling data. It is then combined with the model predictive control to form the course controller. To reduce the influence of wind and waves disturbances, an adaptive weight term is designed in the object function to improve the tracking accuracy of the model predictive control. The guidance commands received by the model predictive controller take into account the path deviation caused by the current and lateral motion of the ship. The results show that GPMPC has the advantages of fast response time and less overshoot; the unmanned sailboat can better achieve waypoint tracking by learning navigation data.
Characterization of the Molecular Mechanisms of Resistance against DMI Fungicides in Cercospora beticola Populations from the Czech Republic
Authors: Kumar Ram , Mazakova Jana , Ali Asad , Sur Vishma Pratap , Sen Madhab Kumar , Bolton Melvin D. , Manasova Marie , Rysanek Pavel , Zouhar Miloslav
Published: December 2021

Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is the most important foliar pathogen of sugar beet worldwide. Extensive reliance on fungicides to manage CLS has resulted in the evolution of fungicide resistance in C. beticola worldwide, including populations in the Czech Republic. One important class of fungicides used to manage CLS is the sterol demethylation inhibitors (DMI). The aim of our study was to assess DMI resistance in C. beticola from the Czech Republic and elucidate the molecular basis of DMI resistance in this population. A total of 50 isolates were collected in 2018 and 2019 from the major sugar beet growing regions of the Czech Republic and assessed for in vitro sensitivity to the DMI fungicides propiconazole, prochloraz, and epoxiconazole. These analyses identified three strains that exhibited 50% effective concentration (EC50) values > 1.0 μg mL–1 against respective fungicides, which were therefore considered resistant. In contrast, strains that exhibited lowest EC50 values were considered sensitive. To explore the molecular basis of resistance in these three strains, the cytochrome P450-dependent sterol 14α-demethylase (Cyp51) gene was sequenced. Sequence analysis identified a Y464S mutation in all three resistant strains. To assess whether Cyp51 gene expression may play a role in DMI resistance, selected strains were grown in vitro with and without fungicide treatment. These analyses indicated that Cyp51 gene expression was significantly induced after fungicide treatment. Thus, we conclude that Y464S point mutation along with induced Cyp51 gene overexpression is likely responsible for resistance against DMI fungicides in C. beticola from the Czech Republic.
Aquatic Hyphomycete Taxonomic Relatedness Translates into Lower Genetic Divergence of the Nitrate Reductase Gene
Authors: Mariz Joana , Franco-Duarte Ricardo , Cássio Fernanda , Pascoal Cláudia , Fernandes Isabel
Published: December 2021

Aquatic hyphomycetes are key microbial decomposers in freshwater that are capable of producing extracellular enzymes targeting complex molecules of leaf litter, thus, being crucial to nutrient cycling in these ecosystems. These fungi are also able to assimilate nutrients (e.g., nitrogen) from stream water, immobilizing these nutrients in the decomposing leaf litter and increasing its nutritional value for higher trophic levels. Evaluating the aquatic hyphomycete functional genetic diversity is, thus, pivotal to understanding the potential impacts of biodiversity loss on nutrient cycling in freshwater. In this work, the inter- and intraspecific taxonomic (ITS1-5.8S-ITS2 region) and functional (nitrate reductase gene) diversity of 40 aquatic hyphomycete strains, belonging to 23 species, was evaluated. A positive correlation was found between the taxonomic and nitrate reductase gene divergences. Interestingly, some cases challenged this trend: Dactylella cylindrospora (Orbiliomycetes) and Thelonectria rubi (Sordariomycetes), which were phylogenetically identical but highly divergent regarding the nitrate reductase gene; and Collembolispora barbata (incertae sedis) and Tetracladium apiense (Leotiomycetes), which exhibited moderate taxonomic divergence but no divergence in the nitrate reductase gene. Additionally, Tricladium chaetocladium (Leotiomycetes) strains were phylogenetically identical but displayed a degree of nitrate reductase gene divergence above the average for the interspecific level. Overall, both inter- and intraspecific functional diversity were observed among aquatic hyphomycetes.
Climate Transition Risk and the Impact on Green Bonds
Authors: Antoniuk Yevheniia , Leirvik Thomas
Published: December 2021

The green bond market develops rapidly and aims to contribute to climate mitigation and adaptation significantly. Green bonds as any asset are subject to transition climate risk, namely, regulatory risk. This paper investigates the impact of unexpected political events on the risk and returns of green bonds and their correlation with other assets. We apply a traditional and regression-based event study and find that events related to climate change policy impact green bonds indices. Green bonds indices anticipated the 2015 Paris Agreement on climate change as a favorable event, whereas the 2016 US Presidential Election had a significant negative impact. The negative impact of the US withdrawal from the Paris agreement is more prominent for municipal but not corporate green bonds. All three events also have a similar effect on green bonds performance in the long term. The results imply that, despite the benefits of issuing green bonds, there are substantial risks that are difficult to hedge. This additional risk to green bonds might cause a time-varying premium for green bonds found in previous literature.
The Impact of Instrumental Stakeholder Management on Blockchain Technology Adoption Behavior in Agri-Food Supply Chains
Authors: Kramer Michael Paul , Bitsch Linda , Hanf Jon H.
Published: December 2021

Coffee is the second most important commodity in terms of global trade value, with its global market value exceeding $460 billion in 2020. Its supply networks, which encompass multiple stakeholders, are complex and nontransparent. Blockchain is a trust technology, and some coffee firms have embraced this technology to provide trust attributes to consumers while making their supply chain more transparent. For businesses to gain the expected productivity advantages, a technology must be adopted and used. As theoretical and empirical research on blockchain technology adoption is scarce, this article attempts to identify behavioral intentions of stakeholders in the supply network toward its adoption. Based on exploratory interviews, this article develops a blockchain technology adoption model based on factors relevant to individuals’ use behavior. The results provide evidence that a normative stakeholder management approach positively impacts use behavior. Managers can use the model to benchmark and improve their corporate social responsibility strategy to obtain better returns on blockchain investments. This study closes a research gap as, to the best of the authors’ knowledge, no research has been conducted so far on the impact of an instrumental stakeholder management approach on blockchain technology adoption behavior. Understanding how stakeholder management can compensate for the lack of consensus mechanisms in private and consortium blockchains, as well as understanding the factors influencing behavioral intentions toward the use of a technology, can provide for managerial guidance toward the development of an effective stakeholder management strategy, which eventually can result in a competitive advantage.
Short-Run Links in Ecological Footprint: A Dynamic Factor Analysis for the EU
Authors: Delgado-Rodríguez María Jesús , Lucas-Santos Sonia de , Cabezas-Ares Alfredo
Published: December 2021

The Ecological Footprint (EFP) is a useful indicator for assessing the progress of environmental performance and offers a solid basis for sustainability studies. In this paper, we contribute to the broadening of its possibilities of investigation by measuring the cross-country links in the EFP in global hectares per capita. The modeling framework is based on the dynamic factor analysis to estimate, in the parametric form, an index that provides information about the short-run dynamics of the EFP in the EU. Following this approach, we identify different patterns in the EFP behavior of the European countries during the period of 1962–2017. The results show stronger links across the EFP of the main European countries: France, Austria, Belgium, Germany, Denmark and the U.K. The proposed analysis gives a better understanding of the links behind environmental degradation in the EU and is applicable for the implementation and design of environmental policies.
Effects of Long-Term Land Use and Land Cover Changes on Ecosystem Service Values: An Example from the Central Rift Valley, Ethiopia
Authors: Mekuria Wolde , Diyasa Merga , Tengberg Anna , Haileslassie Amare
Published: December 2021

Changes in land use and land cover (LULC) are the leading contributors to the decline and loss of ecosystem services in the world. The present study covered the Central Rift Valley lakes basin in Ethiopia, focusing on the valley floor and the East and West escarpments, to analyze changes in LULC and to estimate associated losses in ecosystem service values (ESVs). Covering both upstream and downstream areas in the basin, the study addressed major gaps in existing studies by connecting the sources and sinks of material (e.g., sediment and water) in source-to-lake systems. Additionally, the study facilitated the identification of critical areas for conserving natural resources and reversing the decline of associated ESVs in the Central Rift Valley. A post-classification comparison approach was used to detect LULC changes between 1973 and 2020 using four Landsat images from 1973, 1990, 2005 and 2020. The value transfer valuation method was used to estimate the changes in ESVs due to LULC changes. Among the seven major identified LULC classes, farmlands, settlements, and bare lands showed positive changes, while forestlands, grasslands, shrublands and waterbodies showed negative changes over the last 47 years. The expansion of farmlands, for example, has occurred at the expense of grasslands, forestlands and shrublands. The changes in LULC over a period of 47 years resulted in a total loss of US $62,110.4 × 106 in ESVs. The contributors to the overall loss of ESVs in decreasing order are provisioning services (US $33,795.1 × 106), cultural services (US $28,981.5 × 106) and regulating services (US $652.9 × 106). The results imply that addressing the degradation of land and water resources is crucial to reversing the loss of ecosystem services and achieving the national Sustainable Development Goals (SDGs) related to food and water security (SDGs 2 and 6) and life on land (SDG 15).
Intake of Vitamin D in Patients with Multiple Sclerosis in the Valencian Region and Its Possible Relationship with the Pathogenesis of the Disease
Authors: de la Rubia Ortí Jose Enrique , García María Faus , Drehmer Eraci , Navarro-Illana Esther , Casani-Cubel Julia , Proaño Belén , Sanchis-Sanchis Claudia Emmanuela , Escrivá Juan Doménech
Published: December 2021

(1) Background: Multiple sclerosis (MS) is a neurodegenerative disease characterized by pronounced inflammation. Interleukin 6 (IL-6) is an accurate marker for the state of inflammation, due to the high levels of this cytokine linked to the pathogenesis of the disease. These IL-6 levels could be lowered with an adequate dietary intake of vitamin D. The objective of the study was to determine the level of vitamin D ingested in a sample of patients with MS in the Valencian region (Spain), to establish the vitamin sources, and the possible link between the intake of vitamin D and the pathogenesis of the disease through a relationship with the level of IL-6. (2) Materials and Methods: A descriptive pilot study was carried out with 39 patients with MS in the Valencian region. The dietary-nutritional anamnesis was gained through the food frequency questionnaire (FFQ) and a food diary. Diet and eating habits were analyzed through the Easy Diet (version: 2.0.1)—Consultation Management Program® software, and IL-6 levels in blood by ELISA technique. (3) Results: The results show a low intake of vitamin D, which is significantly and negatively related to the intake of proteins of vegetable origin, which are consumed in less quantity than proteins of animal origin, and significantly and negatively related with the high blood levels of IL-6, possibly as a consequence of the high intake of fats, mainly unsaturated. (4) Conclusions: MS patients in the Valencian region ingest little vitamin D related to low intake of vegetable protein, which would explain the high levels of IL-6 linked to the high intake of mainly saturated fats.
Using a Syrian (Golden) Hamster Biological Model for the Evaluation of Recombinant Anthrax Vaccines
Authors: Kravchenko Tatiana , Titareva Galina , Bakhteeva Irina , Kombarova Tatiana , Borzilov Alexander , Mironova Raisa , Khlopova Kseniya , Timofeev Vitalii
Published: December 2021

In this paper, we demonstrate that a Syrian hamster biological model can be applied to the study of recombinant anthrax vaccines. We show that double vaccination with recombinant proteins, such as protective antigen (PA) and fusion protein LF1PA4, consisting of lethal factor I domain (LF) and PA domain IV, leads to the production of high titers of specific antibodies and to protection from infection with the toxicogenic encapsulated attenuated strain B. anthracis 71/12. In terms of antibody production and protection, Syrian hamsters were much more comparable to guinea pigs than mice. We believe that Syrian hamsters are still underestimated as a biological model for anthrax research, and, in some cases, they can be used as a replacement or at least as a complement to the traditionally used mouse model.
From Life in the Sea to the Clinic: The Marine Drugs Approved and under Clinical Trial
Authors: Cappello Emiliano , Nieri Paola
Published: December 2021

In the last decades Blue Growth policy in european and non-european countries produced a great impulse in applied marine sciences, comprehending the research of new bioactive molecules in marine organisms. These organisms are a great source of natural compounds with unique features resulting from the huge variability of marine habitats and species living in them. Most of the marine compounds in use and in clinical trials are drugs for cancer therapy and many of them are conjugated to antibody to form antibody-drug conjugates (ADCs). Severe pain, viral infections, hypertriglyceridemia, obesity, Alzheimer’s and other CNS diseases are further target conditions for these pharmaceuticals. This review summarizes the state-of-the-art marine drugs focusing on the most successful results in the fast expanding field of marine pharmacology.
Migraine and Its Equivalents: What Do They Share? A Narrative Review on Common Pathophysiological Patterns
Authors: Frattale Ilaria , Ruscitto Claudia , Papetti Laura , Ursitti Fabiana , Sforza Giorgia , Moavero Romina , Ferilli Michela Ada Noris , Tarantino Samuela , Balestri Martina , Vigevano Federico , Mazzone Luigi , Valeriani Massimiliano
Published: December 2021

Migraine is the first in order of frequency of the neurological disorders, affecting both adult and paediatric populations. It is also the first cause of primary headaches in children. Migraine equivalents are periodic disorders that can be associated with migraine or considered as prognostic features of a future migraine manifestation. Despite the mechanisms underlying migraine and its equivalents are not entirely clear, several elements support the hypothesis of common pathophysiological patterns shared by these conditions. The aim of this review is thus to analyze the literature in order to highlight which currently known mechanisms may be common between migraine and its equivalents.
Thermodynamic Scaling of the Shear Viscosity of Lennard-Jones Chains of Variable Rigidity
Authors: Delage Santacreu Stephanie , Hoang Hai , Khennache Samy , Galliero Guillaume
Published: December 2021

In this work, the thermodynamic scaling framework has been used to emphasize the limitations of fully flexible coarse grained molecular models to yield shear viscosity of real liquids. In particular, extensive molecular dynamics simulations have confirmed that, while being reasonable to describe the viscosity of short normal alkanes, the fully flexible Lennard-Jones and Mie chains force fields are inadequate to capture the density dependence of shear viscosity of medium to long alkanes. In addition, it is shown that such a weakness in terms of coarse grained molecular models can be readily quantified by using the thermodynamic scaling framework. As a simple alternative to these force fields, it is demonstrated that the insertion of a variable intramolecular rigidity in the Lennard-Jones chains model exhibits promising results to model medium to long chain-like real fluids from both thermodynamic and viscosity points of view.
CuZn and ZnO Nanoflowers as Nano-Fungicides against Botrytis cinerea and Sclerotinia sclerotiorum: Phytoprotection, Translocation, and Impact after Foliar Application
Authors: Tryfon Panagiota , Kamou Nathalie N. , Mourdikoudis Stefanos , Karamanoli Katerina , Menkissoglu-Spiroudi Urania , Dendrinou-Samara Catherine
Published: December 2021

Inorganic nanoparticles (INPs) have dynamically emerged in plant protection. The uptake of INPs by plants mostly depends on the size, chemical composition, morphology, and the type of coating on their surface. Herein, hybrid ensembles of glycol-coated bimetallic CuZn and ZnO nanoparticles (NPs) have been solvothermally synthesized in the presence of DEG and PEG, physicochemically characterized, and tested as nano-fungicides. Particularly, nanoflowers (NFs) of CuZn@DEG and ZnO@PEG have been isolated with crystallite sizes 40 and 15 nm, respectively. Organic coating DEG and PEG (23% and 63%, respectively) was found to protect the NFs formation effectively. The CuZn@DEG and ZnO@PEG NFs revealed a growth inhibition of phytopathogenic fungi Botrytis cinerea and Sclerotinia sclerotiorum in a dose-dependent manner with CuZn@DEG NFs being more efficient against both fungi with EC50 values of 418 and 311 μg/mL respectively. Lettuce (Lactuca sativa) plants inoculated with S. sclerotiorum were treated with the NFs, and their antifungal effect was evaluated based on a disease index. Plants sprayed with ZnO@PEG NFs showed a relatively higher net photosynthetic (4.70 μmol CO2 m−2s−1) and quantum yield rate (0.72) than with CuZn@DEG NFs (3.00 μmol CO2 m−2s−1 and 0.68). Furthermore, the penetration of Alizarin Red S-labeled NFs in plants was investigated. The translocation from leaves to roots through the stem was evident, while ZnO@PEG NFs were mainly trapped on the leaves. In all cases, no phytotoxicity was observed in the lettuce plants after treatment with the NFs.
On the Development of a Release Mechanism for a Split Hopkinson Tension and Compression Bar
Authors: Baumann Georg , Niederkofler Dominik , Ellersdorfer Christian , Feist Florian
Published: December 2021

Split Hopkinson bars are used for the dynamic mechanical characterisation of materials under high strain rates. Many of these test benches are designed in such a way that they can either be used for compressive or tensile loading. The goal of the present work is to develop a release mechanism for an elastically pre-stressed Split Hopkinson bar that can be universally used for tensile or compressive loading. The paper describes the design and dimensioning of the release mechanism, including the brittle failing wear parts from ultra-high strength steel. Additionally, a numerical study on the effect of the time-to-full-release on the pulse-shape and pulse-rising time was conducted. The results of the analytical dimensioning approaches for the release mechanism, including the wear parts, were validated against experimental tests. It can be demonstrated that the designed release concept leads to sufficiently short and reproducible pulse rising times of roughly 0.11 ms to 0.21 ms, depending on the pre-loading level for both the tension and compression wave. According to literature, the usual pulse rising times can range from 0.01 ms to 0.35 ms, which leads to the conclusion that a good average pulse rising time was achieved with the present release system.
Rehydration Driven Na-Activation of Bentonite—Evolution of the Clay Structure and Composition
Authors: Bahranowski Krzysztof , Klimek Agnieszka , Gaweł Adam , Serwicka Ewa M.
Published: December 2021

A new method of Na-activation of raw bentonite, rich in Ca-montmorillonite, consisting of combined thermal treatment at 200 °C, followed by immediate impregnation with aqueous solution of Na2CO3 of concentration corresponding to 0.5, 1.0, 1.5, or 2.0 cation exchange capacity (CEC) of clay, was investigated. Structural and compositional evolution of the activated solids after 1, 2, 3, and 4 weeks of storage was monitored by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). XRD analysis indicated that within the investigated period of ageing transformation to Na-rich montmorillonite required Na2CO3 concentration of at least 1.0 CEC. FTIR spectra showed that, depending on the Na2CO3 concentration and ageing time, formation of Na-rich montmorillonite was accompanied by precipitation of poorly crystalline calcite, amorphous calcium carbonate, gaylussite (a double calcium-sodium carbonate), and portlandite (Ca(OH)2).
Polylactide Films with the Addition of Olive Leaf Extract—Physico-Chemical Characterization
Authors: Grabska-Zielińska Sylwia , Gierszewska Magdalena , Olewnik-Kruszkowska Ewa , Bouaziz Mohamed
Published: December 2021

The aim of this work was to obtain and characterize polylactide films (PLA) with the addition of poly(ethylene glycol) (PEG) as a plasticizer and chloroformic olive leaf extract (OLE). The composition of OLE was characterized by LC-MS/MS techniques. The films with the potential for using in the food packaging industry were prepared using a solvent evaporation method. The total content of the phenolic compounds and DPPH radical scavenging assay of all the obtained materials have been tested. Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (FTIR-ATR) allows for determining the molecular structure, while Scanning Electron Microscopy (SEM) indicated differences in the films’ surface morphology. Among other crucial properties, mechanical properties, thickness, degree of crystallinity, water vapor permeation rate (WVPR), and color change have also been evaluated. The results showed that OLE contains numerous active substances, including phenolic compounds, and PLA/PEG/OLE films are characterized by improved antioxidant properties. The OLE addition into PLA/PEG increases the material crystallinity, while the WVPR values remain almost unaffected. From these studies, significant insight was gained into the possibility of the application of chloroform as a solvent for both olive leaf extraction and for the preparation of OLE, PLA, and PEG-containing film-forming solutions. Finally, evaporation of the solvent from OLE can be omitted.
Carbon Materials in Electroanalysis of Preservatives: A Review
Authors: Michalkiewicz Slawomir , Skorupa Agata , Jakubczyk Magdalena
Published: December 2021

Electrochemical sensors in electroanalysis are a particularly useful and relatively simple way to identify electroactive substances. Among the materials used to design sensors, there is a growing interest in different types of carbon. This is mainly due to its non-toxic properties, low cost, good electrical conductivity, wide potential range, and the possibility of using it in both aqueous and nonaqueous media. The electrodes made of carbon, and especially of carbon modified with different materials, are currently most often used in the voltammetric analysis of various compounds, including preservatives. The objective of this paper is to present the characteristics and suitability of different carbon materials for the construction of working electrodes used in the voltammetric analysis. Various carbon materials were considered and briefly discussed. Their analytical application was presented on the example of the preservatives commonly used in food, cosmetic, and pharmaceutical preparations. It was shown that for the electroanalysis of preservatives, mainly carbon electrodes modified with various modifiers are used. These modifications ensure appropriate selectivity, high sensitivity, low limits of detection and quantification, as well as a wide linearity range of voltammetric methods of their identification and determination.
High-k Fluoropolymers Dielectrics for Low-Bias Ambipolar Organic Light Emitting Transistors (OLETs)
Authors: Albeltagi Ahmed , Gallegos-Rosas Katherine , Soldano Caterina
Published: December 2021

Organic light emitting transistors (OLETs) combine, in the same device, the function of an electrical switch with the capability of generating light under appropriate bias conditions. In this work, we demonstrate how engineering the dielectric layer based on high-k polyvinylidene fluoride (PVDF)-based polymers can lead to a drastic reduction of device driving voltages and the improvement of its optoelectronic properties. We first investigated the morphology and the dielectric response of these polymer dielectrics in terms of polymer (P(VDF-TrFE) and P(VDF-TrFE-CFE)) and solvent content (cyclopentanone, methylethylketone). Implementing these high-k PVDF-based dielectrics enabled low-bias ambipolar organic light emitting transistors, with reduced threshold voltages (<20 V) and enhanced light output (compared to conventional polymer reference), along with an overall improvement of the device efficiency. Further, we preliminary transferred these fluorinated high-k dielectric films onto a plastic substrate to enable flexible light emitting transistors. These findings hold potential for broader exploitation of the OLET platform, where the device can now be driven by commercially available electronics, thus enabling flexible low-bias organic electronic devices.
Alumina-Toughened-Zirconia with Low Wear Rate in Ball-on-Flat Tribological Tests at Temperatures to 500 °C
Authors: Grabowy Marek , Wojteczko Kamil , Wojteczko Agnieszka , Wiązania Grzegorz , Łuszcz Maciej , Ziąbka Magdalena , Pędzich Zbigniew
Published: December 2021

An alumina-toughened zirconia (ATZ) material, fabricated using a procedure consisting of the common sintering of two different zirconia powders, was tested using the ball-on-disc method in a temperature range between room temperature and 500 °C. Corundum balls were used as a counterpart. The ATZ composite behaviour during tests was compared with that of commonly used α-alumina and tetragonal zirconia sintered samples. At temperatures over 350 °C, a drastic decrease in the wear rate of the material was detected. SEM analyses proved that, in such conditions, nearly the whole surface of the sliding material was covered with a layer of deformed submicrometric grains, which limited contact with the part of material that was not deformed. The mentioned layer was relatively strongly connected with the material, increased its resistance, and decreased its coefficient of friction. As a reference, commonly used materials, namely commercial alumina and tetragonal zirconia, were tested. The wear parameters of the composite were significantly better than those registered for the materials prepared of commercial powders.
Experimental and Numerical Studies on the Behaviors of Autoclaved Aerated Concrete Panels with Insulation Boards Subjected to Wind Loading
Authors: Lu Junkai , Chen Jie , Zhu Kun , Xu Hang , Zhang Wenjia , Deng Qing
Published: December 2021

Autoclaved aerated concrete panels (AACP) are lightweight elements in civil engineering design. In this paper, experiments and numerical analyses were conducted to study the flexural behavior of an enclosure system that consisted of AACPs and a decorative plate. A full-scale test was conducted to investigate the behavior of the enclosure system under wind suction. Load–deflection curves and load–strain relationships under different wind pressures were recorded and discussed. The effects of thickness, reinforcement ratio, and strength grade on the flexural behavior of AACPs were numerically investigated. Based on the numerical results, we found that the flexural behavior of AACPs can be improved by increasing the thickness or the reinforcement ratio. A comparison of finite element and theoretical results calculated using American and Chinese design formulae was conducted, and the results indicated the existing design formulae can conservatively estimate the major mechanical indices of AACPs.
Experimental and FE Study on Impact Strength of Toughened Glass–Retrospective Approach
Authors: Kozłowski Marcin , Zemła Kinga , Kosmal Magda , Kopyłow Ołeksij
Published: December 2021

Due to the high cost of experiments commonly performed to verify the resistance of glass elements to impact loads, numerical models are used as an alternative to physical testing. In these, accurate material parameters are crucial for a realistic prediction of the behaviour of glass panels subjected to impact loads. This applies in particular to the glass’s strength, which is strictly dependent on the strain rate. The article reports the results of an extensive experimental campaign, in which 185 simply supported toughened glass samples were subjected to hard-body impacts. The study covers a wide range of glass thicknesses (from 5 to 15 mm), and it aims to determine a critical drop height causing fracture of the glass. Moreover, a 3D numerical model of the experimental set-up was developed to reproduce the experiments numerically and retrospectively to determine the peak stress in glass that developed during the impact. Based on the results of numerical simulations, a load duration factor of 1.40 for toughened glass for impact loads is proposed. In addition, the paper includes a case study to demonstrate the use of the modelling methodology and results of the work on a practical example of an internal glass partition wall.
Effect of pH-Regulation on the Capture of Lipopolysaccharides from E. coli EH100 by Four-Antennary Oligoglycines in Aqueous Medium
Authors: Gyurova Anna Y. , Berberov Kaloyan , Chinarev Alexander , Nikolov Ljubomir , Karashanova Daniela , Mileva Elena
Published: December 2021

Bacterial lipopolysaccharides (LPS) are designated as endotoxins, because they cause fever and a wide range of pathologies in humans. It is important to develop effective methodologies to detect trace quantities of LPS in aqueous systems. The present study develops a fine-tuning procedure for the entrapment of trace quantities of LPS from E. coli EH100. The capture agents are self-assemblies (tectomers) formed by synthetic four-antennary oligoglycine (C-(CH2-NH-Gly7)4, T4). Based on previously performed investigations of bulk and adsorption-layer properties of aqueous solutions containing T4 and LPS, the optimal conditions for the entrapment interactions are further fine-tuned by the pH regulation of aqueous systems. A combined investigation protocol is developed, including dynamic light scattering, profile analysis tensiometry, microscopic thin-liquid-film techniques, and transmission electron microscopy. The key results are: (1) two types of complexes between T4 and LPS are generated—amphiphilic species and “sandwich-like” hydrophilic entities; the complexes are smaller at lower pH, and larger at higher pH; (2) an optimum range of pH values is established within which the whole quantity of the LPS is entrapped by the tectomers, namely pH = 5.04–6.30. The obtained data substantiate the notion that T4 may be used for an effective capture and the removal of traces of endotoxins in aqueous systems.
Assessment of the Suitability of Elastomeric Bearings Modeling Using the Hyperelasticity and the Finite Element Method
Authors: Gajewski Marcin Daniel , Miecznikowski Mikołaj
Published: December 2021

The paper presents modeling of bridge elastomeric bearings using large deformation theory and hyperelastic constitutive relations. In this work, the simplest neo-Hookean model was compared with the Yeoh model. The parameters of the models were determined from the elastomer uniaxial tensile test and then verified with the results from experimental bearing compression tests. For verification, bearing compression tests were modeled and executed using the finite element method (FEM) in ABAQUS software. Additionally, the parameters of the constitutive models were determined using the inverse analysis method, for which the simulation results were as close as possible to those recorded during the experimental tests. The overall assessment of the suitability of elastomer bearings modeling with neo-Hookean and Yeoh hyperelasticity models is presented in detail.
Panic Spreading Model with Different Emotions under Emergency
Authors: Lv Rongjian , Li Hua , Sun Qiubai
Published: December 2021

Emotion plays an important role in decision making. In an emergency, panic can spread among crowds through person-to-person communications and can cause harmful effects on society. The aim of this paper is to propose a new theoretical model in the context of epidemiology to describe the spread of panic under an emergency. First, according to divisions in personality in the context of psychology, groups are divided into a level-headed group and an impatient group. Second, individuals in the two groups have unique personalities. Thus, the level-headed group only infects within the group, while the impatient group considers emotional infection within the group and cross infection between the groups. Then, a nonlinear infection rate is used to describe the probability of infection after an infected person contacts a susceptible person, which is more in line with the real situation. After that, the level-headed group–impatient group nonlinear SIRS panic spreading model is developed. Stable analysis of the model is obtained using the Lyapunov function method to study the stability of the panic-free equilibrium and panic-permanence equilibrium. Finally, simulations are carried out to dynamically describe the spread process of group emotional contagion.
η-∗-Ricci Solitons and Almost co-Kähler Manifolds
Authors: Sardar Arpan , Khan Mohammad Nazrul Islam , De Uday Chand
Published: December 2021

The subject of the present paper is the investigation of a new type of solitons, called η-∗-Ricci solitons in (k,μ)-almost co-Kähler manifold (briefly, ackm), which generalizes the notion of the η-Ricci soliton introduced by Cho and Kimura. First, the expression of the ∗-Ricci tensor on ackm is obtained. Additionally, we classify the η-∗-Ricci solitons in (k,μ)-ackms. Next, we investigate (k,μ)-ackms admitting gradient η-∗-Ricci solitons. Finally, we construct two examples to illustrate our results.