bluuebunny/crossref_metadata_2025 huggingface dataset:
A Contemporary Model for Industrial Relations
Authors: Ghosh Apoorva , Ray Pranabesh
Published: May 2012

This article attempts to inquire what industrial relations (IR) is and analyzes the ways in which it has been defined and interpreted since its inception. Various scholars ranging from system theorists, pluralists, Marxists and neo- Marxists to the modern HR and strategic choice perspectives have added, modified or questioned its subject matter. An attempt has been made to propose a model for IR so that it can involve all the addressed as well as unaddressed issues. This model is then tested with what have been the scholarly opinions of how IR should change and respond to the contemporary realities. The article concludes by proposing empirical testing of this model in the service economy of post-industrial age when we have knowledge workers instead of blue-collar workers, flat team-based structures instead of hierarchy and participative control rather than bureaucracy. The possibility of IR, given its experience and time-tested understanding of managing workers of huge strength, to borrow the concepts from contemporary disciplines like HRM and behavioural sciences and develop new theories and frameworks that can add meaning to the new realities is also explored.
Is the Sex Communication of Two Pyralid Moths, Plodia interpunctella and Ephestia kuehniella, under Circadian Clock Regulation?
Authors: Závodská Radka , Fexová Silvie , von Wowern Germund , Han Gui-Biao , Dolezel David , Sauman Ivo
Published: May 2012

Females of the Indian meal moth, Plodia interpunctella, and females of the Mediterranean flour month, Ephestia kuehniella (both Lepidoptera: Pyralidae), exhibit daily rhythms in calling behavior. The peak in P. interpunctella calling occurs at dusk, whereas E. kuehniella calls preferentially at dawn. This behavior turned arrhythmic in P. interpunctella females in constant darkness (DD) and remained arrhythmic in constant light (LL), whereas E. kuehniella females showed a persistent rhythm in DD and suppression of the behavior in LL, indicating regulation by a circadian clock mechanism. The rhythm of male locomotor activity corresponded well with the sexual activity of females, reaching the peak at dusk in P. interpunctella and at dawn in E. kuehniella. An immunohistochemical study of the pheromone biosynthesis activating neuropeptide, corazonin, and pigment dispersing factor revealed distinct sets of neurons in the brain-subesophageal complex and in the neurohemal organs of the 2 species.
Light Acts on the Zebrafish Circadian Clock to Suppress Rhythmic Mitosis and Cell Proliferation
Authors: Tamai T. Katherine , Young Lucy C. , Cox Catherine A. , Whitmore David
Published: May 2012

A fundamental role of the circadian clock is to control biochemical and physiological processes such that they occur an optimal time of day. One of the most significant clock outputs from a clinical as well as basic biological standpoint is the timing of the cell cycle. Here we show that the circadian clock regulates the timing of mitosis in a light-responsive, clock-containing zebrafish cell line. Disrupting clock function, using a CLOCK1 dominant-negative construct or constant light, blocks the gating of cell division, demonstrating that this mitotic rhythm is cell autonomous and under control of the circadian pacemaker. Quantitative PCR reveals that several key mitotic genes, including Cyclin B1, Cyclin B2, and cdc2, are rhythmically expressed and clock-controlled. Peak expression of these genes occurs at a critical phase required to gate mitosis to the late night/early morning. Using clock and cell cycle luminescent reporter zebrafish cell lines, we show that light strongly represses not only circadian clock function, but also mitotic gene expression, and consequently slows cell proliferation.
Does Pupil Constriction under Blue and Green Monochromatic Light Exposure Change with Age?
Authors: Daneault Véronique , Vandewalle Gilles , Hébert Marc , Teikari Petteri , Mure Ludovic S. , Doyon Julien , Gronfier Claude , Cooper Howard M. , Dumont Marie , Carrier Julie
Published: May 2012

Many nonvisual functions are regulated by light through a photoreceptive system involving melanopsin-expressing retinal ganglion cells that are maximally sensitive to blue light. Several studies have suggested that the ability of light to modulate circadian entrainment and to induce acute effects on melatonin secretion, subjective alertness, and gene expression decreases during aging, particularly for blue light. This could contribute to the documented changes in sleep and circadian regulatory processes with aging. However, age-related modification in the impact of light on steady-state pupil constriction, which regulates the amount of light reaching the retina, is not demonstrated. We measured pupil size in 16 young (22.8 ± 4 years) and 14 older (61 ± 4.4 years) healthy subjects during 45-second exposures to blue (480 nm) and green (550 nm) monochromatic lights at low (7 × 1012 photons/cm2/s), medium (3 × 1013 photons/cm2/s), and high (1014 photons/cm2/s) irradiance levels. Results showed that young subjects had consistently larger pupils than older subjects for dark adaptation and during all light exposures. Steady-state pupil constriction was greater under blue than green light exposure in both age groups and increased with increasing irradiance. Surprisingly, when expressed in relation to baseline pupil size, no significant age-related differences were observed in pupil constriction. The observed reduction in pupil size in older individuals, both in darkness and during light exposure, may reduce retinal illumination and consequently affect nonvisual responses to light. The absence of a significant difference between age groups for relative steady-state pupil constriction suggests that other factors such as tonic, sympathetic control of pupil dilation, rather than light sensitivity per se, account for the observed age difference in pupil size regulation. Compared to other nonvisual functions, the light sensitivity of steady-state pupil constriction appears to remain relatively intact and is not profoundly altered by age.
Enhancement and Suppression of Ultradian and Circadian Rhythms across the Female Hamster Reproductive Cycle
Authors: Prendergast Brian J. , Beery Annaliese K. , Paul Matthew J. , Zucker Irving
Published: May 2012

The impact of ovarian hormones on hamster ultradian rhythms (URs) is unknown. We concurrently monitored URs and circadian rhythms (CRs) of home cage locomotor activity during the estrous cycle, pregnancy, and lactation of Syrian hamsters. URs with a mean period of 4-5 h were evident during the dark phase in more than 90% of females on days 1 and 2 of the estrous cycle but were significantly less prevalent on cycle days 3 and 4. The period of the UR did not vary as a function of estrous cycle stage, but at all stages, the UR period was longer in the dark than the light phase. The UR acrophase occurred significantly earlier on cycle day 4 than on days 1 and 2, and UR robustness and amplitude were reduced on days 3 and 4. Robustness, mesor, and amplitude of CRs were greater during cycle days 3 and 4; timing of the CR acrophase was delayed on day 4 relative to all other cycle days. Effects of the estrous cycle on URs were evident only during the dark phase. The proportion of hamsters displaying dark phase URs increased significantly during early and late gestation and decreased during lactation. Pregnancy significantly increased UR complexity, robustness, and amplitude. The emergence of URs over gestation was paralleled by decrements in the robustness and amplitude of CRs, which also were absent in a significant proportion of dams during lactation but re-emerged at weaning of litters. The changing endocrine profile of the estrous cycle, hormonal dynamics of pregnancy and lactation, and nursing demands placed on dams are each associated with alterations in the expression of ultradian and circadian locomotor rhythms. Diminution of CRs and augmentation of URs may afford greater behavioral flexibility during life stages when interactions with mates and offspring are less predictable.
Gendered Organizations in the New Economy
Authors: Williams Christine L. , Muller Chandra , Kilanski Kristine
Published: May 2012

Gender scholars draw on the “theory of gendered organizations” to explain persistent gender inequality in the workplace. This theory argues that gender inequality is built into work organizations in which jobs are characterized by long-term security, standardized career ladders and job descriptions, and management controlled evaluations. Over the past few decades, this basic organizational logic has been transformed. In the so-called new economy, work is increasingly characterized by job insecurity, teamwork, career maps, and networking. Using a case study of geoscientists in the oil and gas industry, we apply a gender lens to this evolving organization of work. This article extends Acker’s theory of gendered organizations by identifying the mechanisms that reproduce gender inequality in the twenty-first-century workplace, and by suggesting appropriate policy approaches to remedy these disparities.
Serum procalcitonin has negative predictive value for bacterial infection in active systemic lupus erythematosus
Authors: Bador KM , Intan S , Hussin S , Gafor AHA
Published: May 2012

Background: Previous studies in systemic lupus erythematosus (SLE) patients have produced conflicting results regarding the diagnostic utility of procalcitonin (PCT). The aim of this study was to determine predictive values of PCT and C-reactive protein (CRP) for bacterial infection in SLE patients. Materials and methods: This was a cross-sectional study of clinic and hospitalized SLE patients with and without bacterial infection recruited over 18 months. Bacterial infection was defined as positive culture results. SLE disease activity was measured using SLEDAI. PCT and CRP were measured by automated immunoassays. Results: Sixty-eight patients (57 females) were studied. Ten patients (15%) had infection. The areas under the receiver operating characteristic curves for PCT and CRP were not significantly different [0.797 (CI 0.614–0.979) versus 0.755 (CI 0.600–0.910)]. In lupus flare patients, PCT but not CRP was higher with infection ( p = 0.019 versus 0.195). A PCT of <0.17 ng/ml ruled out infection with 94% negative predictive value (NPV). In remission patients, CRP but not PCT was elevated with infection ( p = 0.036 versus 0.103). CRP < 0.57 mg/dl had 96% NPV. Conclusion: PCT may be a better marker to rule out bacterial infection in lupus flare but not in remission or general screening.
Metaphor as argument: A stylistic genre-based approach
Authors: Badran Dany
Published: May 2012

One of the most intriguing questions in both stylistic and rhetorical analyses relates to determining textual effect on readers, aesthetic or otherwise. Whether the power of the text is directly associated with the role of the text producer and his or her intentions, the linguistic, paralinguistic, extralinguistic and situational context of the text, the background and socio-cognitive expectations of the reader, or a combination of some or all of these factors (or other factors) is a question that is still the subject of stylistic and rhetorical analysis today. This article is a further step in this direction. It attempts to investigate one dimension of textual effect, namely uniformity in reader reaction to an argumentative poem entitled Dinner with the Cannibal, by focusing on the roles that genre and metaphor play in ideologically positioning readers. It argues, on the one hand, that literature is the dominant genre in this hybrid literary-argumentative poem, channelling the readers’ initial interpretations almost exclusively in the interest of more traditional literary interpretative approaches. On the other hand, and more importantly, it focuses on the role that metaphor, as a cognitive link between text producer and reader, plays in the construction of an extremely controlled, uniform interpretation of the argumentative dimension to the poem. The overall effect of the way genre and metaphor function in this argumentative poem, it is concluded, is highly ideological.
Pragmatic acts of love
Authors: Watson Greg
Published: May 2012

This article is a pragmatic examination of the manner in which early American female blues artists talk to their audience about love. It is an extension of the preliminary work of Kuhn (1999), who examined the lyrics of male blues singers, and Watson (2006), who applied a revised version of Tyrmi’s (2004) model to investigate the expression of love and sexuality in the lyrics of pre-1950s’ American female blues singers. Kuhn’s (1999) study concentrated on the seductive strategies of male blues lyrics and applied speech act theory to her corpus. This article aims to extend upon Kuhn’s enquiry, by examining, in greater detail, the seductive strategies and other acts of love expressed in the lyrics sung by early female blues artists. It is, and has generally been, perceived that female artists are less risqué and less assertive in requesting their needs, and that they are more genteel in expressing their desires and feelings. Watson (2006) disproved this assumption. I found that these women were direct in stating their needs, either for love or sexual gratification and had no qualms about stating these needs. In this article, I further investigate how these women express their needs and wants by applying Searle’s (1969, 1976, 1979) speech act theory to the lyrics of early female blues singers. In doing so, I pay particular attention to the use of assertives, directives, and commissives. This methodology is applied to a representatively selected computerised corpus of 111 songs by 39 different American female blues artists, who pre-date the 1950s. Like Kuhn (1999), I am particularly interested in arriving at a fuller understanding of how it is that as we listen to the blues we feel that these lyrics ‘talk’ to us and I am especially interested in proposing a pragmatic taxonomy of these lyrics
Private experience, textual analysis, and institutional authority: The discursive practice of critical interpretation and its enactment in literary training
Authors: Allington Daniel
Published: May 2012

Academic literary criticism emphasises both the private experience of reading and the analysis of formal textual features. Since the early 20th century, this double emphasis has been sustained through the production of ‘readings’ or ‘interpretations’ in which claimed responses to literature are accounted for through textual analysis, a practice here theorised in terms drawn from discursive psychology. Conceptualising interpretation as practice renders it investigable through qualitative social research methods. This article thus studies the enactment of critical interpretation within a specific form of literary training, carrying out a turn-by-turn analysis of an undergraduate tutorial on Oscar Wilde’s The Importance of Being Earnest (1895). Within this classroom context, students use claimed private experiences to challenge a lecturer’s reading of the work, treat those claimed responses (some of which appear homophobic) as unnecessary to account for, and account for responses they reject in non-textual terms. For contrast, a short extract is provided from an established department member’s tutorial on William Wordsworth’s ‘Lines Written a Few Miles above Tintern Abbey’ (1798). It is argued that the two instructors’ very different levels of institutional authority are reflected in their teaching styles and in the resistance or compliance that their students exhibit towards the discursive practices of literary criticism.
Evaluation of monoclonal antibody–based immunohistochemistry for the detection of European and North American Porcine reproductive and respiratory syndrome virus and a comparison with in situ hybridization and reverse transcription polymerase chain reaction
Authors: Han Kiwon , Seo Hwi Won , Oh Yeonsu , Kang Ikjae , Park Changhoon , Kang Sang Hoon , Kim Sung-Hoon , Lee Bog-Hieu , Kwon Byungjoon , Chae Chanhee
Published: May 2012

The objective of the present study was to compare the ability of 2 monoclonal antibodies (mAbs; SDOW17 and SR30) to detect types 1 and 2 Porcine reproductive and respiratory syndrome virus (PRRSV) in formalin-fixed, paraffin-embedded (FFPE) lung tissues by immunohistochemistry (IHC) and to compare the immunohistochemical results with in situ hybridization (ISH) and reverse transcription nested polymerase chain reaction (RT-nPCR) detection techniques. Lungs from 30 experimentally infected pigs (15 pigs with each genotype of PRRSV) and 20 naturally infected pigs (10 pigs with each genotype of PRRSV) with types 1 and 2 PRRSV, respectively, were used for the IHC, ISH, and RT-nPCR analyses. The SR30 mAb-based IHC detected significantly more type 1 PRRSV-positive cells in the accessory and caudal lobes from the experimentally infected pigs at 7 ( P = 0.025) and 14 ( P = 0.018) days postinoculation, respectively, compared to the SDOW17 mAb-based IHC. The results demonstrated that SR30 mAb-based IHC is useful for detecting both types 1 and 2 PRRSV antigen in FFPE lung tissues.
Working Alone Together
Authors: Spinuzzi Clay
Published: May 2012

Mobile professionals can choose to work in offices, executive suites, home offices, or other spaces. But some have instead chosen to work at coworking spaces: open-plan office environments in which they work alongside other unaffiliated professionals for a fee of approximately $250 a month. But what service are they actually purchasing with that monthly fee? How do they describe that service? From an activity theory perspective, what are its object, outcome, and actors? This article reports on a 20-month study that answers such questions.
Development of measurement precision of sensor vibration
Authors: Ghemari Zine , Saad Salah
Published: May 2012

In this paper, an accelerometer mathematical model is developed and simulated to choose the best damping rate value to limit the measurement error to 1%. Using this model, the equations of relative movement modulus and measurement error with respect to damping rate and frequency ratio can be determined. The developed model is validated by a series of tests carried out by computer simulation. The obtained results have shown that a suitable damping rate can minimize the measurement error of relative movement to 1 %. The presented model is a useful tool to improve the damping rate and measurement precision of the accelerometer.
Nonpharmacological Treatments for ADHD
Authors: Hodgson Kristy , Hutchinson Amanda D. , Denson Linley
Published: May 2012

Objective: The authors replicated and expanded on Fabiano et al.’s meta-analysis of behavioral treatments for ADHD, systematically comparing the efficacy of 7 nonpharmacological interventions. Method: A total of 14 controlled treatment studies conducted post-1994—evaluating behavior modification, neurofeedback therapy, multimodal psychosocial treatment, school-based programs, working memory training, parent training, and self-monitoring—were identified, primarily by searching electronic English-language databases. The results were meta-analyzed: mean-weighted effect sizes for the treatment outcomes of 625 participants (382 treatment, 243 controls) were calculated, and moderator analyses examined contributions of gender, ADHD subtype, and treatment “dosage” to outcome. Results: Behavior modification and neurofeedback treatments were most supported by this evidence. Interventions were generally more efficacious for girls, and least efficacious for the “combined” ADHD subtype. The authors found no dose or age effects. Conclusion: Based on the small, published literature, this study supports some nonpharmacological interventions for ADHD, and indicates directions for more evaluation research into psychological treatments.
Reflectivity, reflexivity, reflexivism: IR’s ‘reflexive turn’ — and beyond
Authors: Hamati-Ataya Inanna
Published: May 2012

The notion of ‘reflexivity’ has been so intimately tied to the critique of positivism and empiricism in International Relations (IR) that the emergence of post-positivism has naturally produced the anticipation of a ‘reflexive turn’ in IR theory. Three decades after the launch of the post-positivist critique, however, reflexive IR has failed to impose itself as either a clear or serious contender to mainstream scholarship. Reasons for this failure include: the proliferation of different understandings of ‘reflexivity’ in IR theory that entail significantly different projects and concerns for IR scholarship; the equation of ‘reflexive theory’ with ‘critical’ and ‘emancipatory theory’ and the consequent confusion of ethical/normative issues with strictly epistemic/theoretical ones; and the refusal to consider reflexive IR as a ‘research programme’ concerned with empirical knowledge, not just meta-explanation. The development of reflexivity in IR theory as a sustainable cognitive and praxeological effort is nonetheless possible — and still needed. This article suggests what taking the ‘reflexive turn’ would really entail for IR.
“It’s Like Pay Or Don’t Have It and Now I’m Doing Without”
Authors: Kruszka Bonnie J. , Lindell Deborah , Killion Cheryl , Criss Sam
Published: May 2012

Twenty-four thousand American youth lose Medicaid entitlements after discharge from foster care annually. The circumstance of being uninsured is a formidable barrier to health care that leaves the youth vulnerable to unmet health care needs. Given that foster youth often develop physical and/or mental health problems as a result of abuse or neglect, continuous access to health care is especially important. This descriptive phenomenology study explores the lived experience of transitional uninsured former foster youth. Nine uninsured former foster youth were recruited from a nonprofit community organization in an urban county and interviewed using semistructured interviews. Four themes identified from the analysis were (a) “Surviving the real world”: emancipation without essential documentation; (b) “It’s not always going to be fine”: managing mental and physical health without health care insurance; (c) “Roadblocks”: barriers to securing health care insurance; and (d) “Just not knowing”: Medicaid eligible albeit without health care insurance.
GATA2 zinc finger 1 mutations associated with biallelic CEBPA mutations define a unique genetic entity of acute myeloid leukemia
Authors: Greif Philipp A. , Dufour Annika , Konstandin Nikola P. , Ksienzyk Bianka , Zellmeier Evelyn , Tizazu Belay , Sturm Jutta , Benthaus Tobias , Herold Tobias , Yaghmaie Marjan , Dörge Petra , Hopfner Karl-Peter , Hauser Andreas , Graf Alexander , Krebs Stefan , Blum Helmut , Kakadia Purvi M. , Schneider Stephanie , Hoster Eva , Schneider Friederike , Stanulla Martin , Braess Jan , Sauerland Maria Cristina , Berdel Wolfgang E. , Büchner Thomas , Woermann Bernhard J. , Hiddemann Wolfgang , Spiekermann Karsten , Bohlander Stefan K.
Published: May 2012

Cytogenetically normal acute myeloid leukemia (CN-AML) with biallelic CEBPA gene mutations (biCEPBA) represents a distinct disease entity with a favorable clinical outcome. So far, it is not known whether other genetic alterations cooperate with biCEBPA mutations during leukemogenesis. To identify additional mutations, we performed whole exome sequencing of 5 biCEBPA patients and detected somatic GATA2 zinc finger 1 (ZF1) mutations in 2 of 5 cases. Both GATA2 and CEBPA are transcription factors crucial for hematopoietic development. Inherited or acquired mutations in both genes have been associated with leukemogenesis. Further mutational screening detected novel GATA2 ZF1 mutations in 13 of 33 biCEBPA-positive CN-AML patients (13/33, 39.4%). No GATA2 mutations were found in 38 CN-AML patients with a monoallelic CEBPA mutation and in 89 CN-AML patients with wild-type CEBPA status. The presence of additional GATA2 mutations (n=10) did not significantly influence the clinical outcome of 26 biCEBPA-positive patients. In reporter gene assays, all tested GATA2 ZF1 mutants showed reduced capacity to enhance CEBPA-mediated activation of transcription, suggesting that the GATA2 ZF1 mutations may collaborate with biCEPBA mutations to deregulate target genes during malignant transformation. We thus provide evidence for a genetically distinct subgroup of CN-AML. The German AML cooperative group trials 1999 and 2008 are registered with the identifiers NCT00266136 and NCT01382147 at www.clinicaltrials.gov.
Increased bovine Tim-3 and its ligand expressions during bovine leukemia virus infection
Authors: Okagawa Tomohiro , Konnai Satoru , Ikebuchi Ryoyo , Suzuki Saori , Shirai Tatsuya , Sunden Yuji , Onuma Misao , Murata Shiro , Ohashi Kazuhiko
Published: May 2012

The immunoinhibitory receptor T cell immunoglobulin domain and mucin domain-3 (Tim-3) and its ligand, galectin-9 (Gal-9), are involved in the immune evasion mechanisms for several pathogens causing chronic infections. However, there is no report concerning the role of Tim-3 in diseases of domestic animals. In this study, cDNA encoding for bovine Tim-3 and Gal-9 were cloned and sequenced, and their expression and role in immune reactivation were analyzed in bovine leukemia virus (BLV)-infected cattle. Predicted amino acid sequences of Tim-3 and Gal-9 shared high homologies with human and mouse homologues. Functional domains, including tyrosine kinase phosphorylation motif in the intracellular domain of Tim-3 were highly conserved among cattle and other species. Quantitative real-time PCR analysis showed that bovine Tim-3 mRNA is mainly expressed in T cells such as CD4+ and CD8+ cells, while Gal-9 mRNA is mainly expressed in monocyte and T cells. Tim-3 mRNA expression in CD4+ and CD8+ cells was upregulated during disease progression of BLV infection. Interestingly, expression levels for Tim-3 and Gal-9 correlated positively with viral load in infected cattle. Furthermore, Tim-3 expression level closely correlated with up-regulation of IL-10 in infected cattle. The expression of IFN-γ and IL-2 mRNA was upregulated when PBMC from BLV-infected cattle were cultured with Cos-7 cells expressing Tim-3 to inhibit the Tim-3/Gal-9 pathway. Moreover, combined blockade of the Tim-3/Gal-9 and PD-1/PD-L1 pathways significantly promoted IFN-γ mRNA expression compared with blockade of the PD-1/PD-L1 pathway alone. These results suggest that Tim-3 is involved in the suppression of T cell function during BLV infection.
RETRACTED ARTICLE: Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation
Authors: Kellom Matthew , Basselin Mireille , Keleshian Vasken L , Chen Mei , Rapoport Stanley I , Rao Jagadeesh S
Published: May 2012

Background

Neuroinflammation, caused by six days of intracerebroventricular infusion of bacterial lipopolysaccharide (LPS), stimulates rat brain arachidonic acid (AA) metabolism. The molecular changes associated with increased AA metabolism are not clear. We examined effects of a six-day infusion of a low-dose (0.5 ng/h) and a high-dose (250 ng/h) of LPS on neuroinflammatory, AA cascade, and pre- and post-synaptic markers in rat brain. We used artificial cerebrospinal fluid-infused brains as controls.

Results

Infusion of low- or high-dose LPS increased brain protein levels of TNFα, and iNOS, without significantly changing GFAP. High-dose LPS infusion upregulated brain protein and mRNA levels of AA cascade markers (cytosolic cPLA2-IVA, secretory sPLA2-V, cyclooxygenase-2 and 5-lipoxygenase), and of transcription factor NF-κB p50 DNA binding activity. Both LPS doses increased cPLA2and p38 mitogen-activated protein kinase levels, while reducing protein levels of the pre-synaptic marker, synaptophysin. Post-synaptic markers drebrin and PSD95 protein levels were decreased with high- but not low-dose LPS.

Conclusions

Chronic LPS infusion has differential effects, depending on dose, on inflammatory, AA and synaptic markers in rat brain. Neuroinflammation associated with upregulated brain AA metabolism can lead to synaptic dysfunction.
Culture but not gender modulates amygdala activation during explicit emotion recognition
Authors: Derntl Birgit , Habel Ute , Robinson Simon , Windischberger Christian , Kryspin-Exner Ilse , Gur Ruben C , Moser Ewald
Published: May 2012

Background

Mounting evidence indicates that humans have significant difficulties in understanding emotional expressions from individuals of different ethnic backgrounds, leading to reduced recognition accuracy and stronger amygdala activation. However, the impact of gender on the behavioral and neural reactions during the initial phase of cultural assimilation has not been addressed. Therefore, we investigated 24 Asians students (12 females) and 24 age-matched European students (12 females) during an explicit emotion recognition task, using Caucasian facial expressions only, on a high-field MRI scanner.

Results

Analysis of functional data revealed bilateral amygdala activation to emotional expressions in Asian and European subjects. However, in the Asian sample, a stronger response of the amygdala emerged and was paralleled by reduced recognition accuracy, particularly for angry male faces. Moreover, no significant gender difference emerged. We also observed a significant inverse correlation between duration of stay and amygdala activation.

Conclusion

In this study we investigated the “alien-effect” as an initial problem during cultural assimilation and examined this effect on a behavioral and neural level. This study has revealed bilateral amygdala activation to emotional expressions in Asian and European females and males. In the Asian sample, a stronger response of the amygdala bilaterally was observed and this was paralleled by reduced performance, especially for anger and disgust depicted by male expressions. However, no gender difference occurred. Taken together, while gender exerts only a subtle effect, culture and duration of stay as well as gender of poser are shown to be relevant factors for emotion processing, influencing not only behavioral but also neural responses in female and male immigrants.
Antibiotic prescribing in primary care, adherence to guidelines and unnecessary prescribing - an Irish perspective
Authors: Murphy Marion , Bradley Colin P , Byrne Stephen
Published: May 2012

Background

Information about antibiotic prescribing practice in primary care is not available for Ireland, unlike other European countries. The study aimed to ascertain the types of antibiotics and the corresponding conditions seen in primary care and whether general practitioners (GPs) felt that an antibiotic was necessary at the time of consultation. This information will be vital to inform future initiatives in prudent antibiotic prescribing in primary care.

Methods

Participating GPs gathered data on all antibiotics prescribed by them in 100 consecutive patients’ consultations as well as data on the conditions being treated and whether they felt the antibiotic was necessary.

Results

171 GPs collected data on 16,899 consultations. An antibiotic was prescribed at 20.16% of these consultations. The majority were prescribed for symptoms or diagnoses associated with the respiratory system; the highest rate of prescribing in these consultations were for patients aged 15–64 years (62.23%). There is a high rate of 2nd and 3rd line agents being used for common ailments such as otitis media and tonsillitis. Amoxicillin, which is recommended as 1st line in most common infections, was twice as likely to be prescribed if the prescription was for deferred used or deemed unnecessary by the GP.

Conclusion

The study demonstrates that potentially inappropriate prescribing is occurring in the adult population and the high rate of broad-spectrum antimicrobial agents is a major concern. This study also indicates that amoxicillin may be being used for its placebo effect rather than specifically for treatment of a definite bacterial infection.
The relationship between urban environment and the inflammatory bowel diseases: a systematic review and meta-analysis
Authors: Soon Ing Shian , Molodecky Natalie A , Rabi Doreen M , Ghali William A , Barkema Herman W , Kaplan Gilaad G
Published: May 2012

Background

The objective of this study was to conduct a systematic review with meta-analysis of studies assessing the association between living in an urban environment and the development of the Crohn’s disease (CD) or ulcerative colitis (UC).

Methods

A systematic literature search of MEDLINE (1950-Oct. 2009) and EMBASE (1980-Oct. 2009) was conducted to identify studies investigating the relationship between urban environment and IBD. Cohort and case–control studies were analyzed using incidence rate ratio (IRR) or odds ratio (OR) with 95 % confidence intervals (CIs), respectively. Stratified and sensitivity analyses were performed to explore heterogeneity between studies and assess effects of study quality.

Results

The search strategy retrieved 6940 unique citations and 40 studies were selected for inclusion. Of these, 25 investigated the relationship between urban environment and UC and 30 investigated this relationship with CD. Included in our analysis were 7 case–control UC studies, 9 case–control CD studies, 18 cohort UC studies and 21 cohort CD studies. Based on a random effects model, the pooled IRRs for urban compared to rural environment for UC and CD studies were 1.17 (1.03, 1.32) and 1.42 (1.26, 1.60), respectively. These associations persisted across multiple stratified and sensitivity analyses exploring clinical and study quality factors. Heterogeneity was observed in the cohort studies for both UC and CD, whereas statistically significant heterogeneity was not observed for the case–control studies.

Conclusions

A positive association between urban environment and both CD and UC was found. Heterogeneity may be explained by differences in study design and quality factors.
Relationships between the ABO blood group SNP rs505922 and breast cancer phenotypes: a genotype-phenotype correlation study
Authors: Rummel Seth , Shriver Craig D , Ellsworth Rachel E
Published: May 2012

Background

To date, evaluation of the association of the ABO blood group and breast cancer has yielded mixed results. SNP rs505922, located within the first intron of the ABO gene, has been associated with the adenocarcinoma subtype of pancreatic cancer. To evaluate the association between genetic variation in the ABO blood group and risk of breast cancer, rs505922 was genotyped in 629 Caucasian women with invasive breast cancer, representing a variety of clinical and pathological tumor types.

Methods

Genomic DNA was isolated from blood. TaqMan SNP assay C_2253769_10 was used to determine genotypes for each patient at rs505922. Statistical analysis was performed using chi-square analysis using a P-value <0.05 to define significance.

Results

Genotypes were generated for 100% of the 629 patients in this study. Allele and genotype frequencies did not vary significantly for age at diagnosis, tumor stage, size or grade, hormone, HER2 or lymph node status, intrinsic subtype, tumor type or patient outcome.

Conclusions

Allele frequencies for rs505922 did not differ between women with breast cancer and published HapMap frequencies from women of European descent. Further stratification into different tumor phenotypes also failed to reveal an association between rs505922 and any clinical characteristics. Together, these data suggest that the minor allele of rs505922 and the resulting non-O blood types are not associated with increased risk or less favorable tumor characteristics or prognosis in breast cancer.
Altered small-world properties of gray matter networks in breast cancer
Authors: Hosseini S M Hadi , Koovakkattu Della , Kesler Shelli R
Published: May 2012

Background

Breast cancer survivors, particularly those treated with chemotherapy, are at significantly increased risk for long-term cognitive and neurobiologic impairments. These deficits tend to involve skills that are subserved by distributed brain networks. Additionally, neuroimaging studies have shown a diffuse pattern of brain structure changes in chemotherapy-treated breast cancer survivors that might impact large-scale brain networks.

Methods

We therefore applied graph theoretical analysis to compare the gray matter structural networks of female breast cancer survivors with a history of chemotherapy treatment and healthy age and education matched female controls.

Results

Results revealed reduced clustering coefficient and small-world index in the brain network of the breast cancer patients across a range of network densities. In addition, the network of the breast cancer group had less highly interactive nodes and reduced degree/centrality in the frontotemporal regions compared to controls, which may help explain the common impairments of memory and executive functioning among these patients.

Conclusions

These results suggest that breast cancer and chemotherapy may decrease regional connectivity as well as global network organization and integration, reducing efficiency of the network. To our knowledge, this is the first report of altered large-scale brain networks associated with breast cancer and chemotherapy.
Persistent tumor cells in bone marrow of non-metastatic breast cancer patients after primary surgery are associated with inferior outcome
Authors: Tjensvoll Kjersti , Oltedal Satu , Heikkilä Reino , Kvaløy Jan Terje , Gilje Bjørnar , Reuben James M , Smaaland Rune , Nordgård Oddmund
Published: May 2012

Background

To investigate the prognostic significance of disseminated tumor cells (DTCs) in bone marrow (BM) from non-metastatic breast cancer patients before and after surgery.

Methods

Patients with non-metastatic breast cancer were consecutively recruited to this project during the years 1998–2000. Real-time RT-PCR quantification of a DTC multimarker panel consisting of cytokeratin 19, mammaglobin A and TWIST1 mRNA was performed in BM samples obtained from 154 patients three weeks (BM2) and/or six months after surgery (BM3). The results were compared to previously published data from pre-operative BM analyses for the same patients.

Results

DTCs were identified in post-operative BM samples (BM2 and/or BM3) from 23 (15%) of the 154 patients investigated. During a median follow-up of 98 months, 10 (44%) of these patients experienced systemic relapse as compared to 16 (12%) of 131 DTC-negative patients. Kaplan-Meier estimates of systemic recurrence-free- and breast-cancer specific survival demonstrated significantly shorter survival for patients with persistent DTCs in BM after surgery (p≤0.001). By multivariate Cox regression analyses, persistent DTCs after surgery was an independent predictor of both systemic recurrence-free- (HR = 5.4, p < 0.001) and breast-cancer specific survival (HR = 5.3, p < 0.001). Furthermore, the prognostic value of DTCs in BM was similar for pre- and post surgery samples. However, patients with DTCs both before and after surgery (BM1 and BM2/3) had a particularly poor prognosis (systemic recurrence-free survival: HR = 7.2, p < 0.0001 and breast-cancer specific survival: HR = 8.0, p < 0.0001).

Conclusions

Detection of persistent DTCs in BM samples obtained after surgery identified non-metastatic breast cancer patients at high risk for systemic relapse, and with reduced breast-cancer specific survival. Furthermore, patients with positive DTC status both before and after surgery had a particularly poor prognosis.
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells
Authors: Liang Dongming , Ma Yuanyuan , Liu Jian , Trope Claes Goran , Holm Ruth , Nesland Jahn M , Suo Zhenhe
Published: May 2012

Background

To study whether hypoxia influences the stem-like properties of ovarian cancer cells and their biological behavior under hypoxia.

Method

Ovarian cancer cell lines ES-2 and OVCAR-3 were cultivated in different oxygen tensions for proliferation, cell cycling and invasion analyses. The clonogenic potential of cells was examined by colony formation and sphere formation assays. Stem cell surface markers, SP and CD44brightand CD44dimcells were analyzed by flow cytometry. Protein expression of HIF-1α, HIF-2α, Ot3/4 and Sox2 were investigated by Western blotting.

Results

Both cell lines cultivated at hypoxic condition grew relatively slowly with extended G0/G1 phase. However, if the cells were pre-treated under 1% O2for 48 hrs before brought back to normoxia, the cells showed significantly higher proliferation rate with higher infiltration capability, and significant more colonies and spheres, in comparison to the cells always cultivated under normoxia. CD44brightcells expressed significantly higher levels of Oct3/4 and Sox2 than the CD44dimcells and formed significantly more clones and spheres examinedin vitro. Hypoxic treatment of the cells resulted in stronger CD44 expression in both cell lines, and stronger CD133 expression in the OVCAR-3 cell line. In parallel with these findings, significantly increased number of side population (SP) cells and up-regulated expression of Oct3/4 and Sox2 in both ES-2 and OVCAR-3 cell lines were observed.

Conclusion

We conclude that ovarian cancer cells survive hypoxia by upgrading their stem-like properties through up-regulation of stemness-related factors and behave more aggressively when brought back to higher oxygen environment.
NF-κB2 mutation targets survival, proliferation and differentiation pathways in the pathogenesis of plasma cell tumors
Authors: McCarthy Brian A , Yang Liqun , Ding Jane , Ren Mingqiang , King William , ElSalanty Mohammed , Zakhary Ibrahim , Sharawy Mohamed , Cui Hongjuan , Ding Han-Fei
Published: May 2012

Background

Abnormal NF-κB2 activation has been implicated in the pathogenesis of multiple myeloma, a cancer of plasma cells. However, a causal role for aberrant NF-κB2 signaling in the development of plasma cell tumors has not been established. Also unclear is the molecular mechanism that drives the tumorigenic process. We investigated these questions by using a transgenic mouse model with lymphocyte-targeted expression of p80HT, a lymphoma-associated NF-κB2 mutant, and human multiple myeloma cell lines.

Methods

We conducted a detailed histopathological characterization of lymphomas developed in p80HT transgenic mice and microarray gene expression profiling of p80HT B cells with the goal of identifying genes that drive plasma cell tumor development. We further verified the significance of our findings in human multiple myeloma cell lines.

Results

Approximately 40% of p80HT mice showed elevated levels of monoclonal immunoglobulin (M-protein) in the serum and developed plasma cell tumors. Some of these mice displayed key features of human multiple myeloma with accumulation of plasma cells in the bone marrow, osteolytic bone lesions and/or diffuse osteoporosis. Gene expression profiling of B cells from M-protein-positive p80HT mice revealed aberrant expression of genes known to be important in the pathogenesis of multiple myeloma, including cyclin D1, cyclin D2, Blimp1, survivin, IL-10 and IL-15. In vitro assays demonstrated a critical role of Stat3, a key downstream component of IL-10 signaling, in the survival of human multiple myeloma cells.

Conclusions

These findings provide a mouse model for human multiple myeloma with aberrant NF-κB2 activation and suggest a molecular mechanism for NF-κB2 signaling in the pathogenesis of plasma cell tumors by coordinated regulation of plasma cell generation, proliferation and survival.
An experimental study of VEGF induced changes in vasoactivity in pig retinal arterioles and the influence of an anti-VEGF agent
Authors: Su Er-Ning , Cringle Stephen J , McAllister Ian L , Yu Dao-Yi
Published: May 2012

Background

Vascular endothelial growth factor (VEGF) plays an important role in ocular physiology. Anti-VEGF agents are now used for treatment of common retinal diseases. This study characterises the vasoactive properties of VEGF in isolated perfused pig retinal arterioles under normal tone or endothelin-1 (ET-1) pre-contracted conditions and determines the influence of an anti VEGF agent on VEGF induced vasoactivity.

Methods

An isolated perfused retinal arteriole preparation was used. The outer diameter of retinal vessels was monitored at 2 second intervals in response to VEGF and the anti VEGF agent, bevacizumab. The effect of intraluminal delivery of VEGF was determined over a wide concentration range (10-16 to 10-7 M) both with and without pre-contraction with ET-1 (3 x 10-9 M). Bevacizumab (0.35 mg mL-1) was applied extraluminally to determine the influence of bevacizumab on VEGF induced vasoactive changes on ET-1 pre-contracted vessels.

Results

In retinal arterioles with normal tone, VEGF induced a concentration dependent contraction at low concentrations, reaching 93.5% at 10-11 M and then contraction was reduced at higher concentrations, recovering to 98.1% at 10-7 M. VEGF produced a potent concentration dependent vasodilatation in arterioles pre-contracted with ET-1. VEGF induced vasodilatation in arterioles pre-contracted with ET-1 was significantly inhibited by bevacizumab.

Conclusions

VEGF induced vasoactive changes in pig retinal arterioles are dependent on concentration and vascular tone. Bevacizumab inhibits VEGF-induced vasodilatation in pre-contracted arterioles.
The anticancer effect of saffron in two p53 isogenic colorectal cancer cell lines
Authors: Bajbouj Khuloud , Schulze-Luehrmann Jan , Diermeier Stefanie , Amin Amr , Schneider-Stock Regine
Published: May 2012

Background

Saffron extract, a natural product, has been shown to induce apoptosis in several tumor cell lines. Nevertheless, the p53-dependency of saffron’s mechanism of action in colon cancer remains unexplored.

Material and methods

In order to examine saffron’s anti-proliferative and pro-apoptotic effects in colorectal cancer cells, we treated two p53 isogenic HCT116 cell lines (HCT wildtype and HCT p53−/−) with different doses of the drug and analyzed cell proliferation and apoptosis in a time-dependent manner. MTT viability and crystal violet assays were performed in order to determine the effective dose of saffron on both cell lines. The cell cycle progress was examined by Flow cytometric analysis. Apoptosis was assessed using Annexin-PI-staining and Western Blotting for caspase 3 and PARP cleavage. Autophagy was determined by Western Blotting of the light chain 3 (LC3)-II and Beclin 1 proteins. The protein content of phospho-H2AX (γH2AX), a sensor of DNA double strand breaks, was also analyzed by Western Blotting.

Results

Saffron extract induced a p53-dependent pattern of cell cycle distribution with a full G2/M stop in HCT116 p53 wildtype cells. However, it induced a remarkable delay in S/G2 phase transit with entry into mitosis in HCT116 p53 −/− cells. The apoptotic Pre-G1 cell fraction as well as Annexin V staining and caspase 3 cleavage showed a more pronounced apoptosis induction in HCT116 p53 wildtype cells. Obviously, the significantly higher DNA-damage, reflected by ɣH2AX protein levels in cells lacking p53, was coped by up-regulation of autophagy. The saffron-induced LC3-II protein level was a remarkable indication of the accumulation of autophagosomes, a response to the cellular stress condition of drug treatment.

Conclusions

This is the first study showing the effect of saffron in HCT116 colorectal cancer cells with different p53 status. Saffron induced DNA-damage and apoptosis in both cell lines. However, autophagy has delayed the induction of apoptosis in HCT116 p53 −/− cells. Considering the fact that most tumors show a functional p53 inactivation, further research is needed to elucidate the long-term effects of saffron in p53 −/− tumors.