bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrospun Magnetic Nanocellulose–Polyethersulfone-Conjugated Aspergillus oryzae Lipase for Synthesis of Ethyl Valerate
Authors: Hussin Nurul Hidayah , Wahab Roswanira Abdul , Elias Nursyafiqah , Jacob Adikwu Gowon , Zainal-Abidin Mohamad Hamdi , Abdullah Faizuan , Sulaiman Nurul Jannah , Misson Mailin
Published: December 2021

A novel greener MNC/PES membrane was developed through an electrospinning technique for lipase immobilization to catalyze the synthesis of ethyl valerate (EV). In this study, the covalent immobilization of Aspergillus oryzae lipase (AOL) onto an electrospun nanofibrous membrane consisting of magnetic nanocellulose (MNC) and polyethersulfone (PES) to produce EV was statistically optimized. Raman spectroscopy, Fourier-transform infrared spectroscopy: attenuated total reflection, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, thermal gravimetric analysis (TGA), and differential thermal gravimetric (DTG) of MNC/PES-AOL demonstrated that AOL was successfully immobilized onto the fibers. The Taguchi design-assisted immobilization of AOL onto MNC/PES fibers identified that 1.10 mg/mL protein loading, 4 mL reaction volume, 250 rpm stirring rate, and 50 °C were optimal to yield 72.09% of EV in 24 h. The thermal stability of MNC/PES-AOL was improved by ≈20% over the free AOL, with reusability for up to five consecutive esterification cycles while demonstrating an exceptional half-life of 120 h. Briefly, the electrospun MNC/PES fibers that immobilized AOL showed promising applicability in yielding relatively good EV levels. This study suggests that using MNC as fillers in a PES to improve AOL activity and durability for a longer catalytic process could be a viable option.
Aqueous Dried Extract of Skytanthus acutus Meyen as Corrosion Inhibitor of Carbon Steel in Neutral Chloride Solutions
Authors: Cáceres Luis , Frez Yohana , Galleguillos Felipe , Soliz Alvaro , Gómez-Silva Benito , Borquez Jorge
Published: December 2021

The implementation of corrosion engineering control methods and techniques is crucial to extend the life of urban and industrial infrastructure assets and industrial equipment affected by natural corrosion. Then, the search of stable and environmentally friendly corrosion inhibitors is an important pending task. Here, we provide experimental evidence on the corrosion inhibitory activity of aqueous extracts of Skytanthus acutus Meyen leaf, a native plant from the Atacama Desert in northern Chile. Skytanthus extracts as a powder should be prepared at 55 °C to avoid thermal decomposition and loss of corrosion inhibitory activity. Corrosion of carbon steel AISI1020 immersed in 0.5 M NaCl was evaluated in the presence of different doses of Skytanthus extract by complementary and simultaneous linear polarization, electrochemical impedance spectroscopy, and weight-loss technique under high hydrodynamic conditions. Mixed Potential Theory was applied to confirm the electrochemical activity of the extract inhibitory capabilities. The Skytanthus extracts reached a 90% corrosion inhibitory efficiency when tested at 100 to 1200 ppm in a time span of 48 h, through an electrochemical interaction between the extract inhibitor component and the carbon steel surface. The corrosion inhibition activity observed in Skytanthus dry extracts involves a protective film formation by a mechanism that includes an iron dissolution at the expense of either oxygen reduction and/or hydrogen evolution, followed by a ferrous-ferric iron cycling, the formation of an iron complex and adsorption to the metal surface, and, finally, desorption or degradation of the protecting film. The water-soluble plant extract was subjected to HPLC-MS analyses that rendered 14 major signals, with quinic acid, protocatechuic acid, chlorogenic acid isomers, vanillic acid hexoside, and patuletin 3-methoxy-7-glucoside as the most abundant components. Then, we propose that a phenolic derivative is responsible for the corrosion inhibitory activity found in Skytanthus extracts.
Reconfigurable Disk-like Microswarm under a Sawtooth Magnetic Field
Authors: Zhang Tao , Deng Yuguo , Zhou Bo , Liu Jiayu , Su Yufeng , Li Mu , Zhang Weiwei
Published: December 2021

Swarming robotic systems, which stem from insect swarms in nature, exhibit a high level of environmental adaptability and enhanced tasking capabilities for targeted delivery and micromanipulation. Here, we present a strategy that reconfigures paramagnetic nanoparticles into microswarms energized by a sawtooth magnetic field. A rotary-stepping magnetic-chain mechanism is proposed to address the forming principle of disk-like swarms. Based on programming the sawtooth field, the microswarm can perform reversible transformations between a disk, an ellipse and a ribbon, as well as splitting and merging. In addition, the swarms can be steered in any direction with excellent maneuverability and a high level of pattern stability. Under accurate manipulation of a magnetic microswarm, multiple microparts with complicated shapes were successfully combined into a complete assembly. This reconfigurable swarming microrobot may shed light on the understanding of complex morphological transformations in living systems and provide future practical applications of microfabrication and micromanipulation.
Immuno-Acoustic Sorting of Disease-Specific Extracellular Vesicles by Acoustophoretic Force
Authors: Liu Junyuan , Qu Yuxin , Wang Han
Published: December 2021

Methods for the isolation and analysis of extracellular vesicles (EVs) have been extensively explored in the field of life science and in clinical diagnosis in recent years. The separation and efficient recovery of high-purity target EVs from biological samples are important prerequisites in the study of EVs. So far, commonly used methods of EV separation include ultracentrifugation, filtration, solvent precipitation and immunoaffinity capturing. However, these methods suffer from long processing time, EV damage and low enrichment efficiency. The use of acoustophoretic force facilitates the non-contact label-free manipulation of cells based on their size and compressibility but lacks specificity. Additionally, the acoustophoretic force exerted on sub-micron substances is normally weak and insufficient for separation. Here we present a novel immuno-acoustic sorting technology, where biological substances such as EVs, viruses, and biomolecules, can be specifically captured by antibody/receptor coated microparticles through immunoaffinity, and manipulated by an acoustophoretic force exerted on the microparticles. Using immuno-acoustic sorting technology, we successfully separated and purified HER2-positive EVs for further downstream analysis. This method holds great potential in isolating and purifying specific targets such as disease-related EVs from biological fluids and opens new possibilities for the EV-based early diagnosis and prognosis of diseases.
Design and Development of a Lead-Freepiezoelectric Energy Harvester for Wideband, Low Frequency, and Low Amplitude Vibrations
Authors: Kumari Neetu , Rakotondrabe Micky
Published: December 2021

In recent years, energy harvesting from ambient vibrations using piezoelectric materials has become the center of attention due to the fact that it has the potential to replace batteries, providing an easy way to power wireless and low power sensors and electronic devices. Piezoelectric material has been extensively used in energy harvesting technologies. However, the most commercially available and widely used piezoelectric materials are lead-based, Pb [ZrxTi1−x] O3 (PZT), which contains more than 60 weight percent lead (Pb). Due to its extremely hazardous effects on lead elements, there is a strong need to substitute PZT with new lead-free materials that have comparable properties to those of PZT. Lead-free lithium niobate (LiNbO3) piezoelectric material can be considered as a substitute for lead-based piezoelectric materials for vibrational energy scavenging applications. LiNbO3 crystal has a lower dielectric constant comparison to the conventional piezoceramics (for instance, PZT); however, at the same time, LiNbO3 (LN) single crystal presents a figure of merits similar to that of PZT, which makes it the most suitable choice for a vibrational energy harvester based on lead-free materials. The implementation was carried out using a global optimization approach including a thick single-crystal film on a metal substrate with optimized clamped capacitance for better impedance matching conditions. A lot of research shows that standard designs such as linear piezoelectric energy harvesters are not a prominent solution as they can only operate in a narrow bandwidth because of their single high resonant peak in their frequency spectrum. In this paper, we propose, and experimentally validate, a novel lead-free piezoelectric energy harvester to harness electrical energy from wideband, low-frequency, and low-amplitude ambient vibration. To reach this target, the harvester is designed to combine multi-frequency and nonlinear techniques. The proposed energy harvesting system consists of six piezoelectric cantilevers of different sizes and different resonant frequencies. Each is based on lead-free lithium niobate piezoelectric material coupled with a shape memory alloy (nitinol) substrate. The design is in the form of a circular ring to which the cantilevers are embedded to create nonlinear behavior when excited with ambient vibrations. The finite element simulation and the experimental results confirm that the proposed lead-free harvester design is efficient at low frequencies, particularly different frequencies below 250 Hz.
The Cohesive Energy and Vibration Characteristics of Parallel Single-Walled Carbon Nanotubes
Authors: Wang Jun , Chen Yinfeng , Yu Peishi
Published: December 2021

Based on the van der Waals (vdW) interaction between carbon atoms, the interface cohesive energy between parallel single-walled carbon nanotubes was studied using continuous mechanics theory, and the influence of the diameter of carbon nanotubes and the distance between them on the cohesive energy was analyzed. The results show that the size has little effect on the cohesive energy between carbon nanotubes when the length of carbon nanotubes is over 10 nm. At the same time, we analyzed the cohesive energy between parallel carbon nanotubes with the molecular dynamics simulation method. The results of the two methods were compared and found to be very consistent. Based on the vdW interaction between parallel carbon nanotubes, the vibration characteristics of the two parallel carbon nanotube system were analyzed based on the continuous mechanical Euler-beam model. The effects of the vdW force between carbon nanotubes, the diameter and length of carbon nanotubes on the vibration frequency of carbon nanotubes was studied. The obtained results are helpful in improving the understanding of the vibration characteristics of carbon nanotubes and provide an important theoretical basis for their application.
Highly Efficient Kinetic Resolution of Aryl-Alkenyl Alcohols by Ru-Catalyzed Hydrogen Transfer
Authors: You Yipeng , Jin Ming Yu , Tao Guanyu , Xing Xiangyou
Published: December 2021

No matter through asymmetric reduction of ketones or kinetic resolution of secondary alcohols, enantioselective synthesis of the corresponding secondary alcohols is challenging when the two groups attached to the prochiral or chiral centers are spatially or electronically similar. For examples, dialkyl (sp3 vs. sp3), diaryl (sp2 vs. sp2), and aryl-alkenyl (sp2 vs. sp2) alcohols are difficult to produce with high enantioselectivities. By exploiting our recently developed Ru-catalysts of minimal stereogenicity, we reported herein a highly efficient kinetic resolution of aryl-alkenyl alcohols through hydrogen transfer. This method enabled such versatile chiral building blocks for organic synthesis as allylic alcohols, to be readily accessed with excellent enantiomeric excesses at practically useful conversions.
Laser Fabrication of Nanoholes on Silica through Surface Window Assisted Nano-Drilling (SWAN)
Authors: Lu Yu , Kai Lin , Yang Qing , Du Guangqing , Hou Xun , Chen Feng
Published: December 2021

Nano-structures have significant applications in many fields such as chip fabrications, nanorobotics, and solar cells. However, realizing nanoscale structures on hard and brittle materials is still challenging. In this paper, when processing the silica surface with a tightly focused Bessel beam, the smallest nanohole with ~20 nm diameter has been realized by precisely controlling the interior and superficial interaction of the silica material. An effective surface window assisted nano-drilling (SWAN) mechanism is proposed to explain the generation of such a deep subwavelength structure, which is supported by the simulation results of energy depositions.
Structural and Surfacial Modification of Carbon Nanofoam as an Interlayer for Electrochemically Stable Lithium-Sulfur Cells
Authors: Quay Yee-Jun , Chung Sheng-Heng
Published: December 2021

Electrochemical lithium-sulfur batteries engage the attention of researchers due to their high-capacity sulfur cathodes, which meet the increasing energy-density needs of next-generation energy-storage systems. We present here the design, modification, and investigation of a carbon nanofoam as the interlayer in a lithium-sulfur cell to enable its high-loading sulfur cathode to attain high electrochemical utilization, efficiency, and stability. The carbon-nanofoam interlayer features a porous and tortuous carbon network that accelerates the charge transfer while decelerating the polysulfide diffusion. The improved cell demonstrates a high electrochemical utilization of over 80% and an enhanced stability of 200 cycles. With such a high-performance cell configuration, we investigate how the battery chemistry is affected by an additional polysulfide-trapping MoS2 layer and an additional electron-transferring graphene layer on the interlayer. Our results confirm that the cell-configuration modification brings major benefits to the development of a high-loading sulfur cathode for excellent electrochemical performances. We further demonstrate a high-loading cathode with the carbon-nanofoam interlayer, which attains a high sulfur loading of 8 mg cm−2, an excellent areal capacity of 8.7 mAh cm−2, and a superior energy density of 18.7 mWh cm−2 at a low electrolyte-to-sulfur ratio of 10 µL mg−1.
Magnetically Recoverable Nanoparticulate Catalysts for Cross-Coupling Reactions: The Dendritic Support Influences the Catalytic Performance
Authors: Kuchkina Nina V. , Sorokina Svetlana A. , Bykov Alexey V. , Sulman Mikhail G. , Bronstein Lyudmila M. , Shifrina Zinaida B.
Published: December 2021

Carbon-carbon cross-coupling reactions are among the most important synthetic tools for the preparation of pharmaceuticals and bioactive compounds. However, these reactions are normally carried out using copper, phosphines, and/or amines, which are poisonous for pharmaceuticals. The use of nanocomposite catalysts holds promise for facilitating these reactions and making them more environmentally friendly. In the present work, the PEGylated (PEG stands for poly(ethylene glycol) pyridylphenylene dendrons immobilized on silica loaded with magnetic nanoparticles have been successfully employed for the stabilization of Pd2+ complexes and Pd nanoparticles. The catalyst developed showed excellent catalytic activity in copper-free Sonogashira and Heck cross-coupling reactions. The reactions proceeded smoothly in green solvents at low palladium loading, resulting in high yields of cross-coupling products (from 80% to 97%) within short reaction times. The presence of magnetic nanoparticles allows easy magnetic separation for repeated use without a noticeable decrease of catalytic activity due to the strong stabilization of Pd species by rigid and bulky dendritic ligands. The PEG dendron periphery makes the catalyst hydrophilic and better suited for green solvents. The minor drop in activity upon the catalyst reuse is explained by the formation of Pd nanoparticles from the Pd2+ species during the catalytic reaction. The magnetic separation and reuse of the nanocomposite catalyst reduces the cost of target products as well as energy and material consumption and diminishes residual contamination by the catalyst. These factors as well as the absence of copper in the catalyst makeup pave the way for future applications of such catalysts in cross-coupling reactions.
Ag–Au Core–Shell Triangular Nanoprisms for Improving p-g-C3N4 Photocatalytic Hydrogen Production
Authors: Guo Yali , Xu Anzhou , Hou Juan , Liu Qingcui , Li Hailong , Guo Xuhong
Published: December 2021

Ag–Au core–shell triangular nanoprisms (Ag@Au TNPs) have aroused extensive research interest in the field of hydrogen evolution reaction (HER) due to their strong plasmon effect and stability. Here, Ag@Au TNPs were fabricated by the galvanic-free replacement method. Then, we loaded them on protonated g-C3N4 nanoprisms (P–CN) by the electrostatic self-assembly method as an efficient plasmonic photocatalyst for HER. The hydrogen production rate of Ag@Au TNPs/P–CN (4.52 mmol/g/h) is 4.1 times higher than that of P–CN (1.11 mmol/g/h) under simulated sunlight irradiation, making it the most competitive material for water splitting. The formed Schottky junction helps to trap the hot electrons generated from Ag@Au TNPs, and the well-preserved tips of the Ag@Au TNPs can effectively generate an electromagnetic field to inhibit the photogenerated electron–holes pairs recombination. This study suggests that the rational design of Ag@Au TNPs by the galvanic-free replacement method is an effective co-catalyst for HER and boosting the additional combination of plasmonic metals and catalyst metals for the enhancement to HER.
Super-Repellent Paper Coated with Electrospun Biopolymers and Electrosprayed Silica of Interest in Food Packaging Applications
Authors: Lafraya Alvaro , Prieto Cristina , Pardo-Figuerez Maria , Chiva Alberto , Lagaron Jose M.
Published: December 2021

In the current work, a super-repellent biopaper suitable for food contact applications was developed. To do this, three different kinds of biopolymers, namely polylactide (PLA), poly(ε-caprolactone) (PCL), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and hydrophobic silica microparticles (SiO2), were sequentially processed by electrohydrodynamic processing (EDHP). As a first step, the ultrathin biopolymer fibers were deposited onto a commercial food contact cellulose paper by electrospinning and, thereafter, the nanostructured silica was sequentially electrosprayed. The multilayer coated papers were annealed at different temperatures to promote adhesion between the layers and enhance the super-repellent properties. The developed coatings were characterized in terms of morphology, permeance to water vapor, adhesion, mechanical resistance, and contact and sliding angle. The resultant multilayer biopapers presented a hierarchical micro/nanostructured surface with an apparent water contact angle (WCA) higher than 155° and sliding angle (SA) lower than 10° for all the tested biopolymers used. Among the different multilayer approaches, it was observed that the paper/PHBV/SiO2 showed the best performance, in terms of water vapor permeance; resistance after the tape peeling-off test; and food super-repelling properties to water, yogurt, and custard. Overall, this study presents the successful generation of super-repellent biopapers coated with PLA, PCL, or PHBV along with hydrophobic silica microparticles and its effectiveness for easy emptying food packaging applications to reduce food waste.
The Novel Bone Alkaline Phosphatase Isoform B1x Is Associated with Improved 5-Year Survival in Chronic Kidney Disease
Authors: Haarhaus Mathias , Fernström Anders , Qureshi Abdul Rashid , Magnusson Per
Published: December 2021

Circulating alkaline phosphatase (ALP) is an independent cardiovascular risk marker. Serum bone ALP (BALP) isoforms indicate bone turnover and comprise approximately 50% of total circulating ALP. In chronic kidney disease (CKD), mortality is highest in patients with increased ALP and BALP and low bone turnover. However, not all low bone turnover states are associated with increased mortality. Chronic inflammation and oxidative stress, features of protein energy wasting syndrome, induce cardiovascular BALP activity and fibro-calcification, while bone turnover is suppressed. Circulating BALP isoform B1x is associated with low ALP and low bone turnover and has been exclusively detected in CKD. We investigated the association of serum B1x with survival, abdominal aortic calcification (AAC) score, and aortic pulse wave velocity (PWV) in CKD. Serum ALP, BALP isoforms, parathyroid hormone (PTH), PWV, and AAC were measured repeatedly over 2 years in 68 prevalent dialysis patients. Mortality was assessed after 5 years. B1x was detected in 53 patients. A competing risk analysis revealed an association of B1x with improved 5-year survival; whereas, baseline PWV, but not AAC score, predicted mortality. However, PWV improved in 26 patients (53%), and B1x was associated with variation of PWV over time (p = 0.03). Patients with B1x had lower PTH and total ALP, suggesting an association with lower bone turnover. In conclusion, B1x is associated with time-varying PWV, lower circulating ALP, and improved survival in CKD, and thus may be an indicator of a reduced cardiovascular risk profile among patients with low bone turnover.
Goji Berry Intake Increases Macular Pigment Optical Density in Healthy Adults: A Randomized Pilot Trial
Authors: Li Xiang , Holt Roberta R. , Keen Carl L. , Morse Lawrence S. , Yiu Glenn , Hackman Robert M.
Published: December 2021

Age-related macular degeneration (AMD) is the third leading cause of blindness worldwide. Macular pigment optical density (MPOD), a biomarker for AMD, is a non-invasive measure to assess risk. The macula xanthophyll pigments lutein (L) and zeaxanthin (Z) protect against blue light and provide oxidant defense, which can be indexed by MPOD. This study examined the effects of Z-rich goji berry intake on MPOD and skin carotenoids in healthy individuals. A randomized, unmasked, parallel-arm study was conducted with 27 participants, aged 45–65, who consumed either 28 g of goji berries or a supplement containing 6 mg L and 4 mg Z (LZ), five times weekly for 90 days. After 90 days, MPOD was significantly increased in the goji berry group at 0.25 and 1.75 retinal eccentricities (p = 0.029 and p = 0.044, respectively), while no changes were noted in the LZ group. Skin carotenoids were significantly increased in the goji berry group at day 45 (p = 0.025) and day 90 (p = 0.006), but not in the LZ group. Regular intake of goji berries in a healthy middle-aged population increases MPOD may help prevent or delay the development of AMD.
Selection of Probiotics in the Prevention of Respiratory Tract Infections and Their Impact on Occupational Health: Scoping Review
Authors: Picó-Monllor José Antonio , Ruzafa-Costas Beatriz , Núñez-Delegido Eva , Sánchez-Pellicer Pedro , Peris-Berraco Javier , Navarro-Lopez Vicente
Published: December 2021

The occupational health impact of respiratory infectious diseases is costly to the economy and the health care system. Probiotics are non-pathogenic live microorganisms that, when ingested in adequate amounts, can colonize the intestinal tract, and enhance the immune system. In recent years, numerous studies have described the possible usefulness of certain probiotic strains in the treatment and prevention of respiratory tract infections, with disparate results. In order to assess the possible efficacy and safety of these microorganisms to prevent or ameliorate respiratory tract infections, we systematically searched the bibliographic databases MEDLINE (via Pubmed), EMBASE, The Cochrane library, Scopus, and Web of science, using the descriptors “Respiratory Tract Infections”, “Probiotics”, “Occupational Health”, “Humans”, and “Clinical Trials”. After applying our inclusion and exclusion criteria, 18 studies were accepted for review and critical analysis. Our analysis suggests that a combination of different probiotics, most of them in the genus Bifidobacterium sp. and Lactobacillus sp., could be a good mix to strengthen the immune system and reduce the symptoms of URTIs in the healthy working population.
Progressive Area Elimination of Bovine Brucellosis, 2013–2018, in Gauteng Province, South Africa: Evaluation Using Laboratory Test Reports
Authors: Govindasamy Krpasha , Etter Eric M. C. , Geertsma Peter , Thompson Peter N.
Published: December 2021

Bovine brucellosis is a zoonotic disease of global public health and economic importance. South Africa has had a national bovine brucellosis eradication scheme since 1979; however, no published report on elimination progress from any province exists. We analysed laboratory test results of all cattle herds participating in the Gauteng Provincial Veterinary Services’ eradication scheme between 2013 and 2018. Herd reactor status and within-herd seroprevalence, modelled using mixed-effects logistic and negative binomial regression models, respectively, showed no significant change over the period. However, provincial State Vet Areas, Randfontein (OR = 1.6; 95% CI: 1.2–2.1; p < 0.001) and Germiston (OR = 1.9; 95% CI: 1.5–2.5, p = 0.008) had higher odds of reactor herds than the Pretoria Area and within-herd prevalence count ratios for these areas were 1.5-fold greater than the Pretoria State Vet Area (p < 0.001). Reactor herds were associated with increased herd size (p < 0.001) and larger herd sizes were associated with lower within-herd prevalence (p < 0.001). Despite no evidence of significant progress toward bovine brucellosis elimination in Gauteng province, variability in bovine brucellosis prevalence between State Vet Areas exists. A public health and farmer-supported strategy of ongoing district-based surveillance and cattle vaccination targeting small- to medium-sized herds combined with compulsory test and slaughter of reactors in larger herds is recommended for the province.
Group B Streptococcal Colonization in African Countries: Prevalence, Capsular Serotypes, and Molecular Sequence Types
Authors: Shabayek Sarah , Ferrieri Patricia , Spellerberg Barbara
Published: December 2021

Streptococcus agalactiae or group B streptococcus (GBS) is a commensal of the gastrointestinal and genitourinary tracts of healthy women and an important cause of neonatal invasive infections worldwide. Transmission of bacteria to the newborn occurs at birth and can be prevented by intrapartum antibiotic prophylaxis. However, this not available in resource limited settings in Africa, which carries a particular high burden of disease. Serotype based vaccines are in development and present a suitable alternative to prevent neonatal infections. To be able to assess vaccine efficacy, knowledge and surveillance of GBS epidemiological data are required. This review summarizes investigations about the serotype distribution and the multi-locus sequence types (MLST) found in different African countries. While most serotypes and MLST data are comparable to findings from other continents, some specific differences exist. Serotype V is predominant among colonizing maternal strains in many different African countries. Serotypes that are rarely detected in western industrialized nations, such as serotypes VI, VII and IX, are prevalent in studies from Ghana and Egypt. Moreover, some specific MLST sequence types that seem to be more or less unique to Africa have been detected. However, overall, the data confirm that a hexavalent vaccine can provide broad coverage for the African continent and that a protein vaccine could represent a promising alternative.
A Novel Role for Histatin 5 in Combination with Zinc to Promote Commensalism in C. albicans Survivor Cells
Authors: Norris Hannah , Kumar Rohitashw , Edgerton Mira
Published: December 2021

Candida albicans is maintained as a commensal by immune mechanisms at the oral epithelia. Oral antifungal peptide Histatin 5 (Hst 5) may function in innate immunity, but the specific role Hst 5 plays in C. albicans commensalism is unclear. Since Zn-binding potentiates the candidacidal activity of Hst 5, we hypothesized that Hst 5+Zn would elicit a unique fungal stress response to shape interactions between C. albicans and oral epithelial cells (OECs). We found that Hst 5+Zn but not Hst 5 alone resulted in the activation of cell wall integrity (CWI) signaling, and deletion mutants were then used to determine that CWI-mediated chitin synthesis was protective against killing. Using flow cytometry, we confirmed that Hst 5+Zn-treated cells had significantly elevated levels of cell-wall chitin, mannan and β-1,3 glucan compared to Hst 5-treated cells. We then tested the activation of host signaling components involved in C. albicans cell-wall recognition. The immunoblot assay of C. albicans-exposed oral epithelial cells showed increased activation of EphA2 and NF-κB but not EGFR. Interestingly, C. albicans treated with Hst 5+Zn induced the global suppression of pro-inflammatory cytokine release from OECs, but an increase in negative regulator IL-10. Hst 5+Zn-treated cells were more adherent but ultimately less invasive to OECs than control cells, thus indicating lowered virulence. Therefore, Hst 5+Zn-treated C. albicans cells are discerned by epithelial monolayers, but are less virulent and promote anti-inflammatory signaling, suggesting that Hst 5+Zn in combination could play a role in regulating commensalism of oral C. albicans through cell wall reorganization.
Surveillance of SARS-CoV-2 in Sewage Treatment Plants between January 2020 and July 2021 in Taiwan
Authors: Huang Wei-Lun , Fann Wen-Bin , Shen Rong-Jun , Chu Yi , Yang Jyh-Yuan
Published: December 2021

An outbreak of a new type of coronavirus pneumonia (COVID-19) began in Wuhan, Hubei Province, China, at the end of 2019, and it later spread to other areas of China and around the world. Taiwan reported the first confirmed case from an individual who returned from Wuhan, China, in January 2020 for Chinese New Year. Monitoring microbes in environmental sewage is an important epidemiological indicator, especially for pathogens that can be shed in feces such as poliovirus. We have conducted additional SARS-CoV-2 sewage testing since January 2020 using a well-established poliovirus environmental sewage surveillance system in Taiwan. Wastewater samples were collected from 11 sewage treatment plants from different parts of Taiwan twice a month for laboratory testing. By the end of July 2021, 397 wastewater specimens had been tested, and two samples were positive for SARS-CoV-2. These two wastewater samples were collected in the northern region of Taiwan from Taipei (site A) and New Taipei City (site C) at the beginning of June 2021. This result is consistent with the significant increase in confirmed COVID-19 cases observed in the same period of time. As the pandemic ebbed after June, the wastewater samples in these areas also tested negative for SARS-CoV-2 in July 2021.
PET Imaging in Cardiac Sarcoidosis: A Narrative Review with Focus on Novel PET Tracers
Authors: Saric Petar , Young Kathleen , Rodriguez-Porcel Martin , Chareonthaitawee Panithaya
Published: December 2021

Sarcoidosis is a multi-system inflammatory disease characterized by the development of inflammation and noncaseating granulomas that can involve nearly every organ system, with a predilection for the pulmonary system. Cardiac involvement of sarcoidosis (CS) occurs in up to 70% of cases, and accounts for a significant share of sarcoid-related mortality. The clinical presentation of CS can range from absence of symptoms to conduction abnormalities, heart failure, arrhythmias, valvular disease, and sudden cardiac death. Given the significant morbidity and mortality associated with CS, timely diagnosis is important. Traditional imaging modalities and histologic evaluation by endomyocardial biopsy often provide a low diagnostic yield. Cardiac positron emission tomography (PET) has emerged as a leading advanced imaging modality for the diagnosis and management of CS. This review article will summarize several aspects of the current use of PET in CS, including indications for use, patient preparation, image acquisition and interpretation, diagnostic and prognostic performance, and evaluation of treatment response. Additionally, this review will discuss novel PET radiotracers currently under study or of potential interest in CS.
Discovery of a Novel Template, 7-Substituted 7-Deaza-4′-Thioadenosine Derivatives as Multi-Kinase Inhibitors
Authors: Mashelkar Karishma K. , Byun Woong Sub , Ko Hyejin , Sung Kisu , Tripathi Sushil K. , An Seungchan , Yum Yun A , Kwon Jee Youn , Kim Minjae , Kim Gibae , Kwon Eun-Ji , Lee Hyuk Woo , Noh Minsoo , Lee Sang Kook , Jeong Lak Shin
Published: December 2021

The development of anticancer drugs remains challenging owing to the potential for drug resistance. The simultaneous inhibition of multiple targets involved in cancer could overcome resistance, and these agents would exhibit higher potency than single-target inhibitors. Protein kinases represent a promising target for the development of anticancer agents. As most multi-kinase inhibitors are heterocycles occupying only the hinge and hydrophobic region in the ATP binding site, we aimed to design multi-kinase inhibitors that would occupy the ribose pocket, along with the hinge and hydrophobic region, based on ATP-kinase interactions. Herein, we report the discovery of a novel 4′-thionucleoside template as a multi-kinase inhibitor with potent anticancer activity. The in vitro evaluation revealed a lead 1g (7-acetylene-7-deaza-4′-thioadenosine) with potent anticancer activity, and marked inhibition of TRKA, CK1δ, and DYRK1A/1B kinases in the kinome scan assay. We believe that these findings will pave the way for developing anticancer drugs.
Spin Freezing and Its Impact on Pore Size, Tortuosity and Solid State
Authors: Lammens Joris , Goudarzi Niloofar Moazami , Leys Laurens , Nuytten Gust , Van Bockstal Pieter-Jan , Vervaet Chris , Boone Matthieu N. , De Beer Thomas
Published: December 2021

Spin freeze-drying, as a part of a continuous freeze-drying technology, is associated with a much higher drying rate and a higher level of process control in comparison with batch freeze-drying. However, the impact of the spin freezing rate on the dried product layer characteristics is not well understood at present. This research focuses on the relation between spin-freezing and pore size, pore shape, dried product mass transfer resistance and solid state of the dried product layer. This was thoroughly investigated via high-resolution X-ray micro-computed tomography (µCT), scanning electron microscopy (SEM), thermal imaging and solid state X-ray diffraction (XRD). It was concluded that slow spin-freezing rates resulted in the formation of highly tortuous structures with a high dried-product mass-transfer resistance, while fast spin-freezing rates resulted in lamellar structures with a low tortuosity and low dried-product mass-transfer resistance.
Polymeric Materials for Hemostatic Wound Healing
Authors: Ghimire Suvash , Sarkar Pritha , Rigby Kasey , Maan Aditya , Mukherjee Santanu , Crawford Kaitlyn E. , Mukhopadhyay Kausik
Published: December 2021

Hemorrhage is one of the greatest threats to life on the battlefield, accounting for 50% of total deaths. Nearly 86% of combat deaths occur within the first 30 min after wounding. While external wound injuries can be treated mostly using visual inspection, abdominal or internal hemorrhages are more challenging to treat with regular hemostatic dressings because of deep wounds and points of injury that cannot be located properly. The need to treat trauma wounds from limbs, abdomen, liver, stomach, colon, spleen, arterial, venous, and/or parenchymal hemorrhage accompanied by severe bleeding requires an immediate solution that the first responders can apply to reduce rapid exsanguinations from external wounds, including in military operations. This necessitates the development of a unique, easy-to-use, FDA-approved hemostatic treatment that can deliver the agent in less than 30 s and stop bleeding within the first 1 to 2 min at the point of injury without application of manual pressure on the wounded area.
Cyclodextrin-Modified Nanomaterials for Drug Delivery: Classification and Advances in Controlled Release and Bioavailability
Authors: Real Daniel Andrés , Bolaños Karen , Priotti Josefina , Yutronic Nicolás , Kogan Marcelo J. , Sierpe Rodrigo , Donoso-González Orlando
Published: December 2021

In drug delivery, one widely used way of overcoming the biopharmaceutical problems present in several active pharmaceutical ingredients, such as poor aqueous solubility, early instability, and low bioavailability, is the formation of inclusion compounds with cyclodextrins (CD). In recent years, the use of CD derivatives in combination with nanomaterials has shown to be a promising strategy for formulating new, optimized systems. The goals of this review are to give in-depth knowledge and critical appraisal of the main CD-modified or CD-based nanomaterials for drug delivery, such as lipid-based nanocarriers, natural and synthetic polymeric nanocarriers, nanosponges, graphene derivatives, mesoporous silica nanoparticles, plasmonic and magnetic nanoparticles, quantum dots and other miscellaneous systems such as nanovalves, metal-organic frameworks, Janus nanoparticles, and nanofibers. Special attention is given to nanosystems that achieve controlled drug release and increase their bioavailability during in vivo studies.
Effects of Heat-Treatment on Tensile Behavior and Dimension Stability of 3D Printed Carbon Fiber Reinforced Composites
Authors: Nassar Amal , Younis Mona , Elzareef Mohamed , Nassar Eman
Published: December 2021

This work investigated the effects of heat treatment on the tensile behavior of 3D-printed high modules carbon fiber-reinforced composites. The manufacturing of samples with different material combinations using polylactic acid (PLA) reinforced with 9% carbon fiber (PLACF), acrylonitrile butadiene styrene (ABS) reinforced with 9% carbon fiber (ABSCF) were made. This paper addresses the tensile behavior of different structured arrangements at different% of densities between two kinds of filaments. The comparison of the tensile behavior between heat treated and untreated samples. The results showed that heat treatment improves the tensile properties of samples by enhancing the bonding of filament layers and by reducing the porosity content. At all structure specifications, the rectilinear pattern gives higher strength of up to 33% compared with the Archimedean chords pattern. Moreover, there is a limited improvement in the tensile strength and modulus of elasticity values for the samples treated at low heat-treatment temperature. The suggested methodology to evaluate the tensile behavior of the pairs of materials selected is innovative and could be used to examine sandwich designs as an alternative to producing multi-material components using inexpensive materials.
Preparation and Characterization of Super-Absorbing Gel Formulated from κ-Carrageenan–Potato Peel Starch Blended Polymers
Authors: Moustafa Mahmoud , A. Abu-Saied M. , H. Taha Tarek , Elnouby Mohamed , A. El Desouky Eman , Alamri Saad , Shati Ali , Alrumman Sulaiman , Alghamdii Huda , Al-Khatani Mohmed , Al-Qthanin Rahmah , Al-Emam Ahmed
Published: December 2021

κ-carrageenan is useful for its superior gelling, hydrogel, and thickening properties. The purpose of the study was to maximize the hydrogel properties and water-absorbing capacity of κ-carrageenan by blending it with starch from potato peels to be used as safe and biodegradable water-absorbent children’s toys. The prepared materials were analyzed using FTIR and Raman spectroscopy to analyze the functional groups. Results showed that there was a shift in the characteristic peaks of starch and κ-carrageenan, which indicated their proper reaction during blend formation. In addition, samples show a peak at 1220 cm−1 corresponding to the ester sulfate groups, and at 1670 cm−1 due to the carbonyl group contained in D-galactose. SEM micrographs showed the presence of rough surface topology after blending the two polymers, with the appearance of small pores. In addition, the presence of surface cracks indicates the biodegradability of the prepared membranes that would result after enzymatic treatment. These results are supported by surface roughness results that show the surface of the κ-carrageenan/starch membranes became rougher after enzymatic treatment. The hydrophilicity of the prepared membranes was evaluated from contact angle (CA) measurements and the swelling ratio. The swelling ratio of the prepared membranes increased gradually as the starch ratio increased, reaching 150%, while the water-uptake capacity increased from 48 ± 4% for plain κ-carrageenan to 150 ± 5% for 1:2 κ-carrageenan/starch blends. The amylase enzyme showed an effective ability to degrade both the plain κ-carrageenan and κ-carrageenan/starch membranes, and release glucose units for up to 236 and 563, respectively. According to these results, these blends could be effectively used in making safe and biodegradable molded toys with superior water-absorbing capabilities.
Effect of Finish Line Design on the Fit Accuracy of CAD/CAM Monolithic Polymer-Infiltrated Ceramic-Network Fixed Dental Prostheses: An In Vitro Study
Authors: Baig Mirza Rustum , Akbar Aqdar A. , Embaireeg Munira
Published: December 2021

A polymer-infiltrated ceramic network (PICN) material has recently been introduced for dental use and evidence is developing regarding the fit accuracy of such crowns with different preparation designs. The aim of this in vitro study was to evaluate the precision of fit of machined monolithic PICN single crowns in comparison to lithium disilicate crowns in terms of marginal gap, internal gap, and absolute marginal discrepancies. A secondary aim was to assess the effect of finish line configuration on the fit accuracy of crowns made from the two materials. Two master metal dies were used to create forty stone dies, with twenty each for the two finish lines, shoulder and chamfer. The stone dies were scanned to produce virtual models, on which ceramic crowns were designed and milled, with ten each for the four material–finish line combinations (n = 10). Marginal gaps and absolute marginal discrepancies were evaluated at six pre-determined margin locations, and the internal gap was measured at 60 designated points using a stereomicroscope-based digital image analysis system. The influence of the material and finish line on the marginal and internal adaptation of crowns was assessed by analyzing the data using two-way analysis of variance (ANOVA), non-parametric, and Bonferroni multiple comparison post-hoc tests (α = 0.05). ANOVA revealed that the differences in the marginal gaps and the absolute marginal discrepancies between the two materials were significant (p < 0.05), but that those the finish line effect and the interaction were not significant (p > 0.05). Using the Mann–Whitney U test, the differences in IG for ‘material’ and ‘finish line’ were not found to be significant (p > 0.05). In conclusion, the finish line configuration did not seem to affect the marginal and internal adaptation of PICN and lithium disilicate crowns. The marginal gap of PICN crowns was below the clinically acceptable threshold of 120 µm.
Composites Based on Natural Polymers and Microbial Biomass for Biosorption of Brilliant Red HE-3B Reactive Dye from Aqueous Solutions
Authors: Suteu Daniela , Blaga Alexandra Cristina , Cimpoesu Ramona , Puiţel Adrian Cătălin , Tataru-Farmus Ramona-Elena
Published: December 2021

Natural polymers have proven to be extremely interesting matrices for the immobilization of microbial biomasses, via various mechanisms, in order to bring them into a form easier to handle—the form of composites. This article aimed to study composites based on a residual microbial biomass immobilized in sodium alginate via an encapsulation technique as materials with adsorbent properties. Thus, this study focused on the residual biomass resulting from beer production (Saccharomyces pastorianus yeast, separated after the biosynthesis process by centrifugation and dried at 80 °C)—an important source of valuable compounds, used either as a raw material or for transformation into final products with added value. Thus, the biosorptive potential of this type of composite was tested—presenting in the form of spherical microcapsules 900 and 1500 μm in diameter—in a biosorption process applied to aqueous solutions containing the reactive dye Brilliant Red HE-3B (16.88–174.08 mg/L), studied in a batch system. The preparation and characterization of the obtained polymeric composites (pHPZC, SEM, EDS and FTIR spectra) and an analysis of different equilibrium isotherms (Langmuir, Freundlich and Dubinin-Radushkevich—D–R) were investigated in order to estimate the quantitative characteristic parameters of the biosorption process, its thermal effects, and its possible mechanisms of action. The modelling of the experimental data led to the conclusion that the studied biosorption process took place after reaching the Langmuir isotherm (LI), and that the main mechanism was possibly physical, being spontaneous and probably exothermic according to the values obtained for the free energy of biosorption (E = 8.45–13.608 kJ/mol, from the DR equation), as well as the negative values for the Gibbs free energy and the enthalpy of biosorption (ΔH0 = −87.795 kJ/mol). The results obtained lead to the conclusion that encapsulation of this residual microbial biomass in sodium alginate leads to an easier-to-handle form of biomass, thus being an efficient biosorbent for static or dynamic operating systems for effluents containing moderate concentrations of reactive organic dyes.
Facile Preparation of Tunicate-Inspired Chitosan Hydrogel Adhesive with Self-Healing and Antibacterial Properties
Authors: He Xiang , Liu Ruyue , Liu Huiqing , Wang Ruixiao , Xi Zhenhao , Lin Yixiang , Wang Jie
Published: December 2021

In order to replace traditional wound treatments such as sutures, tissue adhesives with strong wet tissue adhesion and biocompatibility have attracted more attention to the applications of non-invasive wound closure. Herein, inspired by tunicate adhesive protein, a series of 2,3,4-trihydroxybenzaldehyde (TBA)-modified chitosan hydrogels (CS-TBA-Fe) were prepared by easily mixing the solutions of chitosan-FeCl3 and TBA via the Schiff-base reaction and the coordination between Fe3+ and pyrogallol groups. The gelation time was greatly shortened to only several seconds after induced even trace Fe3+. The hydrogel (CS-TBA-Fe) exhibited ~12-fold enhanced wet tissue adhesion strength (60.3 kPa) over the commercial fibrin glue. Meanwhile, the hydrogel also showed robust adhesion to various substrates such as wood, PMMA, and aluminum. The swelling ratio and rheological property can be simply controlled by changing the concentrations of chitosan, TBA, and Fe3+. Moreover, the hydrogel displayed a rapid and highly efficient self-healing ability and an excellent antibacterial activity against E. coli. The overall results show that the CS-TBA-Fe hydrogel with enhanced wet adhesiveness will be a promising tissue adhesive material.