INHIBITORY EFFECT OF CHLOROPHYLL POLYMERIC FORM AGAINST E. СOLI AND S. AUREUS
Authors:   All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences, Moscow 123022, Russian Federation, Gruznov Dmitry V. , Shcherbakova Gulizar Sh. ,   All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences, Moscow 123022, Russian Federation, Popov Nikolai I. ,   All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences, Moscow 123022, Russian Federation, Shuteeva Еkaterina N. ,   All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences, Moscow 123022, Russian Federation, Konyashkina Alina V. ,   All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences, Moscow 123022, Russian Federation, Gruznova Olga A. ,   N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences, Moscow 119991, Russian Federation, Lobanov Anton V. ,   N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences, Moscow 119991, Russian Federation; Moscow Pedagogical State University, Moscow 119991, Russian Federation, Chesnokova Irina P. ,   N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences, Moscow 119991, Russian Federation; MIREA – Russian Technological University, Moscow 119454, Russian Federation
Published: September 2024

The increase in the number of antibiotic-resistant strains of microorganisms is becoming more widespread. This problem can be solved using photodynamic and light-independent therapy. Metalloporphyrins have been successfully used in both fields for many years. One of the most wellknown representatives of porphyrins is chlorophyll (Chl). The purpose of this study is to develop a polymeric form of Chl by incorporating it into polymer – poly-N-vinylpyrrolidone (PVP), and to study its inhibitory effect against Staphylococcus aureus (strain 209-P) and Escherichia coli (strain 1257). A method for obtaining the Chl polymeric form is presented, and еру analysis of its incorporation into the polymeric matrix is carried out. It was shown that Chl and Chl-PVP at a dose of 60 μg had a significant inhibitory effect on the growth of S. aureus. The effect on E. coli growth was significantly lower even when using the highest concentration tested. The Chl-PVP complex showed a prolonged effect compared to the free form of Chl. The data obtained can be used in the development of antibacterial dosage forms.