bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Grape Polyphenols on the Life Span and Neuroinflammatory Alterations Related to Neurodegenerative Parkinson Disease-Like Disturbances in Mice
Authors: Tikhonova Maria A. , Tikhonova Nadezhda G. , Tenditnik Michael V. , Ovsyukova Marina V. , Akopyan Anna A. , Dubrovina Nina I. , Amstislavskaya Tamara G. , Khlestkina Elena K.
Published: November 2020

Functional nutrition is a valuable supplementation to dietary therapy. Functional foods are enriched with biologically active substances. Plant polyphenols attract particular attention due to multiple beneficial properties attributed to their high antioxidant and other biological activities. We assessed the effect of grape polyphenols on the life span of C57BL/6 mice and on behavioral and neuroinflammatory alterations in a transgenic mouse model of Parkinson disease (PD) with overexpression of the A53T-mutant human α-synuclein. C57BL/6 mice were given a dietary supplement containing grape polyphenol concentrate (GPC—1.5 mL/kg/day) with drinking water from the age of 6–8 weeks for life. Transgenic PD mice received GPC beginning at the age of 10 weeks for four months. GPC significantly influenced the cumulative proportion of surviving and substantially augmented the average life span in mice. In the transgenic PD model, the grape polyphenol (GP) diet enhanced memory reconsolidation and diminished memory extinction in a passive avoidance test. Behavioral effects of GP treatment were accompanied by a decrease in α-synuclein accumulation in the frontal cortex and a reduction in the expression of neuroinflammatory markers (IBA1 and CD54) in the frontal cortex and hippocampus. Thus, a GP-rich diet is recommended as promising functional nutrition for aging people and patients with neurodegenerative disorders.
Exploring the Proteomic Alterations from Untreated and Cryoablation and Irradiation Treated Giant Cell Tumors of Bone Using Liquid-Chromatography Tandem Mass Spectrometry
Authors: Madda Rashmi , Chen Chao-Ming , Chen Cheng-Fong , Wang Jir-You , Wu Po-Kuei , Chen Wei-Ming
Published: November 2020

Giant cell tumors of bone (GCT) are benign tumors that show a locally aggressive nature and affect bones’ architecture. Recently, cryoablation and irradiation treatments have shown promising results in GCT patients with faster recovery and less recurrence and metastasis. Therefore, it became a gold standard surgical treatment for patients. Hence, we have compared GCT-untreated, cryoablation, and irradiation-treated samples to identify protein alterations using high-frequency liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Our label-free quantification analysis revealed a total of 107 proteins (p < 0.01) with 26 up-regulated (< 2-folds to 5-fold), and 81 down-regulated (> 0.1 to 0.5 folds) proteins were identified from GCT-untreated and treated groups. Based on pathway analysis, most of the identified up-regulated proteins involved in critical metabolic functions associated with tumor proliferation, angiogenesis, and metastasis. On the other hand, the down-regulated proteins involved in glycolysis, tumor microenvironment, and apoptosis. The observed higher expressions of matrix metalloproteinase 9 (MMP9) and TGF-beta in the GCT-untreated group associated with bones’ osteolytic process. Interestingly, both the proteins showed reduced expressions after cryoablation treatment, and contrast expressions identified in the irradiation treated group. Therefore, these expressions were confirmed by immunoblot analysis. In addition to these, several glycolytic enzymes, immune markers, extracellular matrix (ECM), and heat shock proteins showed adverse expressions in the GCT-untreated group were identified with favorable regulations after treatment. Therefore, the identified expression profiles will provide a better picture of treatment efficacy and effect on the molecular environment of GCT.
The Influence of Carbon Nature on the Catalytic Performance of Ru/C in Levulinic Acid Hydrogenation with Internal Hydrogen Source
Authors: Jędrzejczyk Marcin , Soszka Emilia , Goscianska Joanna , Kozanecki Marcin , Grams Jacek , Ruppert Agnieszka M.
Published: November 2020

The influence of the nature of carbon materials used as a support for Ru/C catalysts on levulinic acid hydrogenation with formic acid as a hydrogen source toward gamma-valerolactone was investigated. It has been shown that the physicochemical properties of carbon strongly affect the catalytic activity of Ru catalysts. The relationship between the hydrogen mobility, strength of hydrogen adsorption, and catalytic performance was established. The catalyst possessing the highest number of defects, stimulating metal support interaction, exhibited the highest activity. The effect of the catalyst grain size was also studied. It was shown that the decrease in the grain size resulted in the formation of smaller Ru crystallites on the catalyst surface, which facilitates the activity.
The Role of Titanium Dioxide on the Hydration of Portland Cement: A Combined NMR and Ultrasonic Study
Authors: Diamantopoulos George , Katsiotis Marios , Fardis Michael , Karatasios Ioannis , Alhassan Saeed , Karagianni Marina , Papavassiliou George , Hassan Jamal
Published: November 2020

Titanium dioxide (TiO2) is an excellent photocatalytic material that imparts biocidal, self-cleaning and smog-abating functionalities when added to cement-based materials. The presence of TiO2 influences the hydration process of cement and the development of its internal structure. In this article, the hydration process and development of a pore network of cement pastes containing different ratios of TiO2 were studied using two noninvasive techniques (ultrasonic and NMR). Ultrasonic results show that the addition of TiO2 enhances the mechanical properties of cement paste during early-age hydration, while an opposite behavior is observed at later hydration stages. Calorimetry and NMR spin–lattice relaxation time T1 results indicated an enhancement of the early hydration reaction. Two pore size distributions were identified to evolve separately from each other during hydration: small gel pores exhibiting short T1 values and large capillary pores with long T1 values. During early hydration times, TiO2 is shown to accelerate the formation of cement gel and reduce capillary porosity. At late hydration times, TiO2 appears to hamper hydration, presumably by hindering the transfer of water molecules to access unhydrated cement grains. The percolation thresholds were calculated from both NMR and ultrasonic data with a good agreement between both results.
Evaluation of an Element-Tagged Duplex Immunoassay Coupled with Inductively Coupled Plasma Mass Spectrometry Detection: A Further Study for the Application of the New Assay in Clinical Laboratory
Authors: Jiang Wencan , Sun Gongwei , Cui Wenbin , Men Shasha , Jing Miao , Pu Danna , Zhang Sichun , Yuan Xiaozhou , Zhang Xinrong , Wang Chengbin
Published: November 2020

Background: Element-tagged immunoassay coupled with inductively coupled plasma mass spectrometry (ICP-MS) detection has the potential to revolutionize immunoassay analysis for multiplex detection. However, a further study referring to the standard evaluation and clinical sample verification is needed to ensure its reliability for simultaneous analysis in clinical laboratories. Methods: Carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) were chosen for the duplex immunoassay. The performance of the assay was evaluated according to guidelines from the Clinical and Laboratory Standards Institute (CLSI). Moreover, reference intervals (RIs) of CEA and AFP were established. At last, 329 clinical samples were analyzed by the proposed method and results were compared with those obtained with electrochemiluminescent immunoassay (ECLIA) method. Results: The measurement range of the assay was 2–940 ng/mL for CEA and 1.5–1000 ng/mL for AFP, with a detection limit of 0.94 ng/mL and 0.34 ng/mL, respectively. The inter-assay and intra-assay imprecision were all less than 6.58% and 10.62%, respectively. The RI of CEA and AFP was 0–3.84 ng/mL and 0–9.94 ng/mL, respectively. Regarding to clinical sample detection, no significant difference was observed between the proposed duplex assay and the ECLIA method. Conclusions: The ICP-MS-based duplex immunoassay was successfully developed and the analytical performance fully proved clinical applicability. Well, this could be different with other analytes.
Comparative Studies on Regioselectivity of α- and β-Linked Glucan Tosylation
Authors: Koschella Andreas , Heinze Thomas , Tied Antje , Geitel Katja , Chien Chih-Ying , Iwata Tadahisa
Published: November 2020

Alpha- and beta-linked 1,3-glucans have been subjected to conversion with p-toluenesulfonic acid (tosyl) chloride and triethylamine under homogeneous reaction conditions in N,N-dimethyl acetamide/LiCl. Samples with a degree of substitution of tosyl groups (DSTs) of up to 1.91 were prepared by applying 5 mol reagent per mole repeating unit. Hence, the reactivity of α-1,3-glucan is comparable with cellulose and starch, while the β-1,3-linked glucan curdlan is less reactive. The samples dissolve in aprotic dipolar media independent of the DSTs and possess a solubility in less polar solvents that depends on the DSTs. NMR studies on the tosyl glucans and of the peracylated derivatives showed a preferred tosylation of position 2 of the repeating unit. However, the selectivity is less pronounced compared with starch. It could be concluded that the α-configurated glycosidic bond directs tosyl groups towards position 2.
M Mode Ultrasound and Tissue Doppler Imaging to Assess Diaphragm Feature in Late Onset Pompe Disease
Authors: Meng Paris , Ogna Adam , Fayssoil Abdallah
Published: November 2020

Late-onset Pompe disease (LOPD) is an autosomal recessive lysosomal storage disease. Clinical features include skeletal muscle deficiency and diaphragm weakness. Clinical management relies on supportive treatment and mechanical ventilation in patients with chronic respiratory failure. M mode ultrasound and sniff tissue Doppler imaging can be used to assess and follow diaphragm function.
Polygenetic-Risk Scores Related to Crystallin Metabolism Are Associated with Age-Related Cataract Formation and Interact with Hyperglycemia, Hypertension, Western-Style Diet, and Na Intake
Authors: Jee Donghyun , Kang Suna , Huang ShaoKai , Park Sunmin
Published: November 2020

Age-related cataract (ARC) development is associated with loss of crystalline lens transparency related to interactions between genetic and environmental factors. We hypothesized that polygenetic risk scores (PRS) of the selected genetic variants among the ARC-related genes might reveal significant genetic impacts on ARC risk, and the PRS might have gene–gene and gene–lifestyle interactions. We examined the hypothesis in 1972 and 39,095 subjects aged ≥50 years with and without ARC, respectively, in a large-scale hospital-based cohort study conducted from 2004 to 2013. Single nucleotide polymorphisms (SNPs) of the genes related to ARC risk were identified, and polygenetic risk scores (PRS) were generated based on the results of a generalized multifactor dimensionality reduction analysis. Lifestyle interactions with PRS were evaluated. The PRS derived from the best model included the following six SNPs related to crystallin metabolism: ULK4_rs1417380362, CRYAB_rs2070894, ACCN1_rs55785344, SSTR2_rs879419608, PTN_rs322348, and ICA1_rs200053781. The risk of ARC in the high-PRS group was 2.47-fold higher than in the low-PRS group after adjusting for confounders. Age, blood pressure, and glycemia interacted with PRS to influence the risk of ARC: the incidence of ARC was much higher in the elderly (≥65 years) and individuals with hypertension or hyperglycemia. The impact of PRS on ARC risk was greatest in middle-aged individuals with hypertension or hyperglycemia. Na, coffee, and a Western-style diet intake also interacted with PRS to influence ARC risk. ARC risk was higher in the high-PRS group than in the low-PRS group, and high Na intake, Western-style diet, and low coffee intake elevated its risk. In conclusion, ARC risk had a positive association with PRS related to crystallin metabolism. The genetic impact was greatest among those with high Na intake or hypertension. These results can be applied to precision nutrition interventions to prevent ARC.
Presence of the HPPD Inhibitor Sensitive 1 Gene and ALSS653N Mutation in Weedy Oryza sativa Sensitive to Benzobicyclon
Authors: Brabham Chad , Norsworthy Jason K. , González-Torralva Fidel
Published: November 2020

Benzobicyclon has shown varying results in controlling weedy rice, including those with imidazolinone (IMI) resistance. Tolerance to benzobicyclon in cultivated japonica rice, but not indica or aus-like cultivars, is conferred by a fully functional HPPD Inhibitor Sensitive 1 (HIS1) gene. Herein, a diagnostic Kompetitive Allele Specific PCR (KASP) assay was developed to predict the HIS1 genotype of weedy rice plants from 37 accessions and correlated to their response to benzobicyclon in the field. Two-thirds of the 693 weedy rice plants screened were tolerant to benzobicyclon (371 g ai ha−1, SC formulation) at 30 days after treatment (DAT). Thirty-four percent of plants were homozygous for the HIS1 allele and 98% of these plants exhibited field tolerance. However, the his1 genotype did not always correlate with field data. Only 52% of his1 plants were considered sensitive, indicating that the single nucleotide polymorphisms (SNPs) chosen in the KASP assay are not a reliable tool in predicting his1 homozygous plants. In an additional experiment, 86% of the 344 plants with at least one copy of the ALSS653N trait harbored a HIS1 allele, suggesting fields infested with IMI herbicide-resistant weedy rice are unlikely to be controlled with benzobicyclon.
Direct and Bicarbonate-Induced Iron Deficiency Differently Affect Iron Translocation in Kiwifruit Roots
Authors: Wang Nannan , Dong Xiaoke , Chen Yuanlei , Ma Baiquan , Yao Chunchao , Ma Fengwang , Liu Zhande
Published: November 2020

Bicarbonate-induced iron (Fe) deficiency (+Bic) is frequently observed in kiwifruit orchards, but more research attention has been paid to direct Fe deficiency (-Fe) in plants, including kiwifruit. Here we compared the differences of kiwifruit plants between -Fe and +Bic in: (1) the traits of 57Fe uptake and translocation within plants, (2) Fe forms in roots, and (3) some acidic ions and metabolites in roots. The concentration of 57Fe derived from nutrient solution (57Fedfs) in roots was less reduced in +Bic than -Fe treatment, despite similar decrease in shoots of both treatments. +Bic treatment increased 57Fedfs distribution in fine roots but decreased it in new leaves and stem, thereby displaying the inhibition of 57Fedfs translocation from roots to shoots and from fine roots to xylem of coarse roots. Moreover, +Bic imposition induced the accumulation of water-soluble Fe and apoplastic Fe in roots. However, the opposite was observed in -Fe-treated plants. Additionally, the cell wall Fe and hemicellulose Fe in roots were less reduced by +Bic than -Fe treatment. +Bic treatment also triggered the reduction in H+ extrusion and the accumulation of NH4+, succinic acid, and some amino acids in roots. These results suggest that, contrary to -Fe, +Bic treatment inhibits Fe translocation to shoots by accumulating water-soluble and apoplastic Fe and slowing down the release of hemicellulose Fe in the cell wall in kiwifruit roots, which may be related to the decreased H+ extrusion and the imbalance between C and N metabolisms.
Effect of Nardostachys jatamansi DC. on Apoptosis, Inflammation and Oxidative Stress Induced by Doxorubicin in Wistar Rats
Authors: Singh Mhaveer , Khan Mohammad Ahmed , Y. T. Kamal , Ahmad Javed , Fahmy Usama A. , Kotta Sabna , Alhakamy Nabil A. , Ahmad Sayeed
Published: November 2020

The study aimed to investigate the protective action of jatamansi (Nardostachys jatamansi DC.) against doxorubicin cardiotoxicity. Methanolic extract of jatamansi (MEJ) was prepared and standardized using HPTLC fingerprinting, GC-MS chemoprofiling, total phenolic content, and antioxidant activity in vitro. Further in vivo activity was evaluated using rodent model. Animals were divided into five groups (n = 6) namely control (CNT) (Normal saline), toxicant (TOX, without any treatment), MEJ at low dose (JAT1), MEJ at high dose (JAT2), and standard desferrioxamine (STD). All groups except control received doxorubicin 2.5 mg per Kg intra-peritoneally for 3 weeks in twice a week regimen. After 3 weeks, the blood samples and cardiac tissues were collected from all groups for biochemical and histopathological evaluation. Treatment with MEJ at both dose levels exhibited significant reduction (p < 0.001 vs. toxicant) of serum CK-MB (heart creatine kinase), LDH (Lactate dehydrogenase) & HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) levels, and tissue MDA (melondialdehyde) level; insignificant difference was observed (p > 0.05) in TNF-alpha (tumour necrosis factor), IL-6 (interleukine-6) levels and caspase activity as compared to TOX. Histopathological evaluation of cardiac tissues of different treatment groups further reinforced the findings of biochemical estimation. This study concludes that jatamansi can protect cardiac tissues from oxidative stress-induced cell injury and lipid peroxidation as well as against inflammatory and apoptotic effects on cardiac tissues.
Rice Breeding in Russia Using Genetic Markers
Authors: Dubina Elena , Kostylev Pavel , Ruban Margarita , Lesnyak Sergey , Krasnova Elena , Azarin Kirill
Published: November 2020

The article concentrates on studying tolerance to soil salinization, water flooding, and blast in Russian and Asian rice varieties, as well as hybrids of the second and third generations from their crossing in order to obtain sustainable paddy crops based on domestic varieties using DNA markers. Samples IR 52713-2B-8-2B-1-2, IR 74099-3R-3-3, and NSIC Rc 106 were used as donors of the SalTol tolerance gene. Varieties with the Sub1A locus were used as donors of the flood resistance gene: Br-11, CR-1009, Inbara-3, TDK-1, and Khan Dan. The lines C101-A-51 (Pi-2), C101-Lac (Pi-1, Pi-33), IR-58 (Pi-ta), and Moroberekan (Pi-b) were used to transfer blast resistance genes. Hybridization of the stress-sensitive domestic varieties Novator, Flagman, Virazh, and Boyarin with donor lines of the genes of interest was carried out. As a result of the studies carried out using molecular marking based on PCR in combination with traditional breeding, early-maturing rice lines with genes for resistance to salinity (SalTol) and flooding (Sub1A), suitable for cultivation in southern Russia, were obtained. Introgression and pyramiding of the blast resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, and Pi-b into the genotypes of domestic rice varieties were carried out. DNA marker analysis revealed disease-resistant rice samples carrying 5 target genes in a homozygous state. The created rice varieties that carry the genes for blast resistance (Pentagen, Magnate, Pirouette, Argamac, Kapitan, and Lenaris) were submitted for state variety testing. The introduction of such varieties into production will allow us to avoid epiphytotic development of the disease, preserving the biological productivity of rice and obtaining environmentally friendly agricultural products.
Inactivation of H+-ATPase Participates in the Influence of Variation Potential on Photosynthesis and Respiration in Peas
Authors: Yudina Lyubov , Sherstneva Oksana , Sukhova Ekaterina , Grinberg Marina , Mysyagin Sergey , Vodeneev Vladimir , Sukhov Vladimir
Published: November 2020

Local damage (e.g., burning, heating, or crushing) causes the generation and propagation of a variation potential (VP), which is a unique electrical signal in higher plants. A VP influences numerous physiological processes, with photosynthesis and respiration being important targets. VP generation is based on transient inactivation of H+-ATPase in plasma membrane. In this work, we investigated the participation of this inactivation in the development of VP-induced photosynthetic and respiratory responses. Two- to three-week-old pea seedlings (Pisum sativum L.) and their protoplasts were investigated. Photosynthesis and respiration in intact seedlings were measured using a GFS-3000 gas analyzer, Dual-PAM-100 Pulse-Amplitude-Modulation (PAM)-fluorometer, and a Dual-PAM gas-exchange Cuvette 3010-Dual. Electrical activity was measured using extracellular electrodes. The parameters of photosynthetic light reactions in protoplasts were measured using the Dual-PAM-100; photosynthesis- and respiration-related changes in O2 exchange rate were measured using an Oxygraph Plus System. We found that preliminary changes in the activity of H+-ATPase in the plasma membrane (its inactivation by sodium orthovanadate or activation by fusicoccin) influenced the amplitudes and magnitudes of VP-induced photosynthetic and respiratory responses in intact seedlings. Decreases in H+-ATPase activity (sodium orthovanadate treatment) induced fast decreases in photosynthetic activity and increases in respiration in protoplasts. Thus, our results support the effect of H+-ATPase inactivation on VP-induced photosynthetic and respiratory responses.
Nematicidal Activity of Essential Oils on a Psychrophilic Panagrolaimus sp. (Nematoda: Panagrolaimidae)
Authors: Oro Violeta , Krnjajic Slobodan , Tabakovic Marijenka , Stanojevic Jelena S. , Ilic-Stojanovic Snezana
Published: November 2020

Essential oils (EOs) have historically been used for centuries in folk medicine, and nowadays they seem to be a promising control strategy against wide spectra of pathogens, diseases, and parasites. Studies on free-living nematodes are scarce. The free-living microbivorous nematode Panagrolaimus sp. was chosen as the test organism. The nematode possesses extraordinary biological properties, such as resistance to extremely low temperatures and long-term survival under minimal metabolic activity. Fifty EOs from 22 plant families of gymnosperms and angiosperms were tested on Panagrolaimus sp. The aims of this study were to investigate the in vitro impact of EOs on the psychrophilic nematode Panagrolaimus sp. in a direct contact bioassay, to list the activity of EOs based on median lethal concentration (LC50), to determine the composition of the EOs with the best nematicidal activity, and to compare the activity of EOs on Panagrolaimus sp. versus plant parasitic nematodes. The results based on the LC50 values, calculated using Probit analysis, categorized the EOs into three categories: low, moderate and highly active. The members of the laurel family, i.e., Cinnamomum cassia and C. burmannii, exhibited the best nematicidal activity. Aldehydes were generally the major chemical components of the most active EOs and were the chemicals potentially responsible for the nematicidal activity.
Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana
Authors: Antonić Dragana D. , Subotić Angelina R. , Dragićević Milan B. , Pantelić Danijel , Milošević Snežana M. , Simonović Ana D. , Momčilović Ivana
Published: November 2020

Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.
Microstructure and Mechanical Properties of PU/PLDL Sponges Intended for Grafting Injured Spinal Cord
Authors: Lis-Bartos Anna , Szarek Dariusz , Krok-Borkowicz Małgorzata , Marycz Krzysztof , Jarmundowicz Włodzimierz , Laska Jadwiga
Published: November 2020

Highly porous, elastic, and degradable polyurethane and polyurethane/polylactide (PU/PLDL) sponges, in various shapes and sizes, with open interconnected pores, and porosity up to 90% have been manufactured. They have been intended for gap filling in the injured spinal cord. The porosity of the sponges depended on the content of polylactide, i.e., it decreased with the increase of polylactide content. The rise of polylactide content caused an increase of Young modulus and rigidity as well as a more complex morphology of the polyurethane/polylactide blends. The mechanical properties, in vitro toxicity, and degradation in artificial cerebrospinal fluid were tested. Sponges underwent continuous degradation with varying degradation rates depending on the polymer composition. In vitro cell studies with fibroblast cultures proved the biocompatibility of the polymers. Based on the obtained results, the designed PU/PLDL sponges appeared to be promising candidates for bridging gaps within injured spinal cord in further in vitro and in vivo studies.
3D Polyaniline Nanofibers Anchored on Carbon Paper for High-Performance and Light-Weight Supercapacitors
Authors: Rahman Sami ur , Röse Philipp , Surati Mit , Shah Anwar ul Haq Ali , Krewer Ulrike , Bilal Salma
Published: November 2020

In the field of advanced energy storage, nanostructured Polyaniline (PANI) based materials hold a special place. Extensive studies have been done on the application of PANI in supercapacitors, however, the structure–property relationship of these materials is still not understood. This paper presents a detailed characterization of the novel sodium phytate doped 3D PANI nanofibers anchored on different types of carbon paper for application in supercapacitors. An excellent relationship between the structures and properties of the synthesized samples was found. Remarkable energy storage characteristics with low values of solution, charge transfer and polarization resistance and a specific capacitance of 1106.9 ± 1.5 F g−1 and 779 ± 2.6 F g−1 at current density 0.5 and 10 Ag−1, respectively, was achieved at optimized conditions. The symmetric supercapacitor assembly showed significant enhancement in both energy density and power density. It delivered an energy density of 95 Wh kg−1 at a power of 846 W kg−1. At a high-power density of 16.9 kW kg−1, the energy density can still be kept at 13 Wh kg−1. Cyclic stability was also checked for 1000 cycles at a current density of 10 Ag−1 having excellent retention, i.e., 96%.
Optimization of Adhesion Strength and Microstructure Properties by Using Response Surface Methodology in Enhancing the Rice Husk Ash-Based Geopolymer Composite Coating
Authors: Mohd Basri Mohd Salahuddin , Mustapha Faizal , Mazlan Norkhairunnisa , Ishak Mohd Ridzwan
Published: November 2020

As a result of their significant importance and applications in vast areas, including oil and gas, building construction, offshore structures, ships, and bridges, coating materials are regularly exposed to harsh environments which leads to coating delamination. Therefore, optimum interfacial bonding between coating and substrate, and the reason behind excellent adhesion strength is of utmost importance. However, the majority of studies on polymer coatings have used a one-factor-at-a-time (OFAT) approach. The main objective of this study was to implement statistical analysis in optimizing the factors to provide the optimum adhesion strength and to study the microstructure of a rice husk ash (RHA)-based geopolymer composite coating (GCC). Response surface methodology was used to design experiments and perform analyses. RHA/alkali activated (AA) ratio and curing temperature were chosen as factors. Adhesion tests were carried out using an Elcometer and a scanning electron microscope was used to observe the microstructure. Results showed that an optimum adhesion strength of 4.7 MPa could be achieved with the combination of RHA/AA ratio of 0.25 and curing temperature at 75 °C. The microstructure analysis revealed that coating with high adhesion strength had good interfacial bonding with the substrate. This coating had good wetting ability in which the coating penetrated the valleys of the profiles, thus wetting the entire substrate surface. A large portion of dense gel matrix also contributed to the high adhesion strength. Conversely, a large quantity of unreacted or partially reacted particles may result in low adhesion strength.
Synthesis and Cytotoxicity Study of Magnetite Nanoparticles Coated with Polyethylene Glycol and Sorafenib–Zinc/Aluminium Layered Double Hydroxide
Authors: Ebadi Mona , Buskaran Kalaivani , Bullo Saifullah , Hussein Mohd Zobir , Fakurazi Sharida , Pastorin Giorgia
Published: November 2020

In the last two decades, the development of novel approaches for cancer treatment has attracted intense attention due to the growing number of patients and the inefficiency of the available current conventional treatments. In this study, superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by the co-precipitation method in an alkaline medium. Then the nanoparticles were chemically modified by coating them with polyethylene glycol (PEG) and sorafenib (SO)–zinc/aluminum layered double hydroxide (ZLDH) to improve their biocompatibility. The SPIONs and their coated and drug-loaded nanoparticles, M-PEG–SO–ZLDH are of the crystalline phase with the presence of C, O, Al, Fe, Cl, Zn in the latter, indicating the presence of the coating layers on the surface of the SPIONs. The superparamagnetic properties of the bare SPIONs were found to be reduced but retained in its coated drug delivery nanoparticles, M-PEG–SO–ZLDH. The latter has an average particle size of 16 nm and the release of the drug from it was found to be governed by the pseudo-second-order kinetic. The cytotoxicity and biocompatibility evaluation of the drug-loaded magnetic nanoparticles using 3T3 and HepG2 cells using the diphenyltetrazolium bromide (MTT) assays shows that the synthesized nanoparticles were less toxic than the pure drug. This preliminary study indicates that the prepared nanoparticles are suitable to be used for the drug delivery system.
Characteristics of Glycerolized Chitosan: NH4NO3-Based Polymer Electrolyte for Energy Storage Devices with Extremely High Specific Capacitance and Energy Density Over 1000 Cycles
Authors: Aziz Shujahadeen B. , Brza M. A. , Brevik Iver , Hamsan M. H. , Abdulwahid Rebar T. , Majid S. R. , Kadir M. F. Z. , Hussen Sarkawt A. , M. Abdullah Ranjdar
Published: November 2020

In this work, plasticized polymer electrolyte films consisting of chitosan, ammonium nitrate (NH4NO3) and glycerol for utilization in energy storage devices was presented. Various microscopic, spectroscopic and electrochemical techniques were used to characterize the concerned electrolyte and the electrical double-layer capacitor (EDLC) assembly. The nature of complexation between the polymer electrolyte components was examined via X-ray diffraction analysis. In the morphological study, field emission scanning electron microscopy (FESEM) was used to investigate the impact of glycerol as a plasticizer on the morphology of films. The polymer electrolyte (conducting membrane) was found to have a conductivity of 3.21 × 10−3 S/cm. It is indicated that the number density (n), mobility (μ) and diffusion coefficient (D) of ions are increased with the glycerol amount. The mechanism of charge storing was clarified, which implies a non-Faradaic process. The voltage window of the polymer electrolyte is 2.32 V. It was proved that the ion is responsible for charge-carrying via measuring the transference number (TNM). It was also determined that the internal resistance of the EDLC assembly lay between 39 and 50 Ω. The parameters associated with the EDLC assembly are of great importance and the specific capacitance (Cspe) was determined to be almost constant over 1 to 1000 cycles with an average of 124 F/g. Other decisive parameters were found: energy density (18 Wh/kg) and power density (2700 W/kg).
Water Vapor Pathways during Freeze-Drying of Foamed Product Matrices Stabilized by Maltodextrin at Different Concentrations
Authors: Kubbutat Peter , Tauchnitz Annika , Kulozik Ulrich
Published: November 2020

This study aimed to identify the water-vapor transport mechanisms through an aerated matrix during microwave freeze-drying. Due to the larger surface area and lower water vapor transport resistance of an aerated product compared to the solution, foam structures dry faster. Different foam structures were produced with different maltodextrin (MD) concentrations (10–40%) as a foam-stabilizing agent. Depending on the initial viscosity of the solution prior to foaming, the samples differed in overrun (41–1671%) and pore size (d50 = 58–553 µm). Experiments were partially performed in a freeze-drying chamber of a light microscope to visualize structural changes in-situ. Different mechanisms were identified explaining the accelerated drying of foams, depending on the MD concentration, above or below 30%. At lower MD concentration, high overruns could be produced prior to freezing with big bubbles and thin lamellae with short diffusion pathway length. At 40% MD concentration, the viscosity was too high to integrate much air into the product. Therefore, the foam overrun was low and the bubble size small. Under these conditions, the water vapor generates high pressure, resulting in the formation of channels between bubbles, thus creating the pathways with low resistance for a very fast water vapor mass transfer. In addition, microwave freeze-drying experiments using a pilot plant unit were conducted to validate the findings of the freeze-drying microscope. A reduction of the drying time from 150 min (10% MD) to 78 min (40% MD) was achieved.
Collaborative Control Applied to BSM1 for Wastewater Treatment Plants
Authors: Morales-Rodelo Keidy , Francisco Mario , Alvarez Hernan , Vega Pastora , Revollar Silvana
Published: November 2020

This paper describes a design procedure for a collaborative control structure in Plant Wide Control (PWC), taking into account the existing controllable parameters as a novelty in the procedure. The collaborative control structure includes two layers, supervisory and regulatory, which are determined according to the dynamics hierarchy obtained by means of the Hankel matrix. The supervisory layer is determined by the main dynamics of the process and the regulatory layer comprises the secondary dynamics and controllable parameters. The methodology proposed is applied to a wastewater treatment plant, particularly to the Benchmark Simulation Model No 1 (BSM1) for the activated sludge process, comparing the results with the use of a Model Predictive Controller in the supervisory layer. For determining controllable parameters in the BSM1 control, a new specific oxygen mass transfer model in the biological reactor has been developed, separating the kLa volumetric mass transfer coefficient into two controllable parameters, kL and a.
Novel Hemocompatible Imine Compounds as Alternatives for Antimicrobial Therapy in Pharmaceutical Application
Authors: Altamimi Mohammad A. , Hussain Afzal , Alshehri Sultan , Imam Syed Sarim , Alnami Abdulmalik , Bari Ahmed
Published: November 2020

The aim of this study was to synthesize, characterize, and evaluate neoteric imine compounds for antimicrobial activity and hemocompatibility. Four compounds were synthesized using 3-thiophene carboxaldehyde, ethanol, amine, and acetic acid. The compounds were characterized using nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), and powder X-ray diffraction (PXRD). A solubility study was conducted with various solvents and surfactants at 40 °C. An in vitro antimicrobial assay was performed against bacterial and fungal strains to determine the zone of inhibition and minimum inhibitory concentrations. Finally, an in vitro hemolysis study was conducted using rat erythrocytes. The structure of the compounds was confirmed by NMR, FTIR corroborated their functional group attributes, DSC determined their enthalpies of fusion and fusion temperatures, and PXRD confirmed their crystalline nature. These compounds were water-insoluble but soluble in chloroform, with a maximum solubility of ~80 mg/mL. The antimicrobial assay suggested that two of the products exerted potent activities against C. albicans and several bacterial strains. Finally, hemolysis analysis excluded the possibility of hemolysis at the assessed concentrations. In conclusion, two of the novel imine compounds showed promise as antimicrobial agents to control local and systemic microbial infections in a suitable dosage form.
Tetracycline and Sulfonamide Antibiotics in Soils: Presence, Fate and Environmental Risks
Authors: Conde-Cid Manuel , Núñez-Delgado Avelino , Fernández-Sanjurjo María , Álvarez-Rodríguez Esperanza , Fernández-Calviño David , Arias-Estévez Manuel
Published: November 2020

Veterinary antibiotics are widely used worldwide to treat and prevent infectious diseases, as well as (in countries where allowed) to promote growth and improve feeding efficiency of food-producing animals in livestock activities. Among the different antibiotic classes, tetracyclines and sulfonamides are two of the most used for veterinary proposals. Due to the fact that these compounds are poorly absorbed in the gut of animals, a significant proportion (up to ~90%) of them are excreted unchanged, thus reaching the environment mainly through the application of manures and slurries as fertilizers in agricultural fields. Once in the soil, antibiotics are subjected to a series of physicochemical and biological processes, which depend both on the antibiotic nature and soil characteristics. Adsorption/desorption to soil particles and degradation are the main processes that will affect the persistence, bioavailability, and environmental fate of these pollutants, thus determining their potential impacts and risks on human and ecological health. Taking all this into account, a literature review was conducted in order to shed light on the current knowledge about the occurrence of tetracycline and sulfonamide antibiotics in manures/slurries and agricultural soils, as well as on their fate in the environment. For that, the adsorption/desorption and the degradation (both abiotic and biotic) processes of these pollutants in soils were deeply discussed. Finally, the potential risks of deleterious effects on human and ecological health associated with the presence of these antibiotic residues were assessed. This review contributes to a deeper understanding of the lifecycle of tetracycline and sulfonamide antibiotics in the environment, thus facilitating decision-making for the application of preventive and mitigation measures to reduce its negative impacts and risks to public health.
A Wavelet Transform-Assisted Convolutional Neural Network Multi-Model Framework for Monitoring Large-Scale Fluorochemical Engineering Processes
Authors: Li Xintong , Zhou Kun , Xue Feng , Chen Zhibing , Ge Zhiqiang , Chen Xu , Song Kai
Published: November 2020

The barely satisfactory monitoring situation of the hypertoxic fluorochemical engineering processes requires the application of advanced strategies. In order to deal with the non-linear mechanism of the processes and the highly complicated correlation among variables, a wavelet transform-assisted convolutional neural network (CNN) based multi-model dynamic monitoring method was proposed. A preliminary CNN model was first trained to detect faults and to diagnose part of them with minimum computational burden and time delay. Then, a wavelet assisted secondary CNN model was trained to diagnose the remaining faults with the highest possible accuracy. In this step, benefitting from the scale decomposition capabilities of the wavelet transform function, the inherent noise and redundant information could be filtered out and the useful signal was transformed into a higher compact space. In this space, a well-designed secondary CNN model was trained to further improve the fault diagnosis performance. The application on a refrigerant-producing process located in East China showed that not only regular faults but also hard to diagnose faults were successfully detected and diagnosed. More importantly, the unique online queue assembly updating strategy proposed remarkably reduced the inherent time delay of the deep-learning methods. Additionally, the application of it on the widely used Tennessee Eastman process benchmark strongly proved the superiority of it in fault detection and diagnosis over other deep-learning methods.
Multi-Temporal InSAR Parallel Processing for Sentinel-1 Large-Scale Surface Deformation Mapping
Authors: Duan Wei , Zhang Hong , Wang Chao , Tang Yixian
Published: November 2020

Interferometric synthetic aperture radar (InSAR) has achieved great success in various geodetic applications, and its potential for ground deformation measurements on the large scale has attracted increasingly more attention in recent years. The increasing number of synthetic aperture radar (SAR) satellite systems have steadily provided a large amount of SAR data. Among these systems, the Sentinel-1 mission can be considered a milestone in the development of InSAR techniques, offering new opportunities to monitor global surface deformation with high precision, due to its wide coverage, short revisit time, and free access. However, conventional InSAR techniques have encountered great challenges in large-scale InSAR processing over wide areas because of the large computational burden and complexity. In this work, we present a novel parallel computing-based coherent scatterer InSAR (P-CSInSAR) method for automatic and efficient generation of deformation results from Sentinel-1 raw data. To achieve high parallelization performance for the overall InSAR processing chain, parallelization strategies at different levels have been adopted in the P-CSInSAR method, which has been fully addressed in this work. To evaluate the efficiency and accuracy of the proposed method, P-CSInSAR has been tested on the North China Plain regions with three adjacent frames of Sentinel-1 images, and the deformation results have been validated by GPS measurements. The experimental results confirm the effectiveness of the proposed parallel computing-based P-CSInSAR method. The proposed method can also play an important role in exploiting Sentinel-1 InSAR big data for disaster prevention and reduction.
Remote Sensing of Ice Conditions in the Southeastern Baltic Sea and in the Curonian Lagoon and Validation of SAR-Based Ice Thickness Products
Authors: Kozlov Igor E. , Krek Elena V. , Kostianoy Andrey G. , Dailidienė Inga
Published: November 2020

Here we analyze ice conditions in the Southeastern Baltic (SEB) Sea and in the Curonian Lagoon (CL) using spaceborne synthetic aperture radar (SAR) data combined with in-situ measurements from coastal stations during four winter seasons between 2009–2013. As shown, the ice conditions in the SEB and in the CL are strongly varying from year to year and do not always correlate with each other. In the SEB, ice cover may form only within 5–15 km band along the coast or spread up to 100 km offshore covering almost the entire region. The mean ice season duration here is 45 days. The CL is almost fully ice-covered every year apart of its northern part subjected to sea water inflow and active shipping. The ice regime is also more stable here, however, it also possesses multiple periods of partial melting and re-freezing. In this study we also perform a validation of three SAR-based ice thickness products (Envisat ASAR 0.5-km and 1-km, and RADARSAT-2 0.5-km) produced by the Finnish Meteorological Institute versus in-situ measurements in the CL. As shown, all satellite products perform rather well for the periods of gradual ice thickness growth. When the ice thickness grows rapidly, all products underestimate the observed values by 10–20 cm (20–50%). The best results were obtained for the RADARSAT-2 ice thickness product with the highest R2 value (0.68) and the root mean square error around 8 cm. The results of the study clearly show that multi-mission SAR data are very useful for spatial and temporal analysis of the ice regime in coastal waters and semi-enclosed shallow water bodies where the number of field observations is insufficient or lacking.
Spatial-Temporal Evolution of Land Subsidence and Rebound over Xi’an in Western China Revealed by SBAS-InSAR Analysis
Authors: Shi Wei , Chen Guan , Meng Xingmin , Jiang Wanyu , Chong Yan , Zhang Yi , Dong Ying , Zhang Maosheng
Published: November 2020

Land subsidence is one of the major urban geological hazards, which seriously restricts the development of many cities in the world. As one of the major cities in China, Xi’an has also been experiencing a large area of land subsidence due to excessive exploitation of groundwater. Since the Heihe Water Transfer Project (HWTP) became fully operational in late 2003, the problem of subsidence has been restrained, but other issues, such as ground rebounds, have appeared, and the effect of the underground space utilization on land subsidence remains unsolved. The spatial-temporal pattern of land subsidence and rebound in Xi’an after HWTP and their possible cause have so far not been well understood. In this study, the evolutionary characteristics of land subsidence and rebound in Xi’an city from 2007–2019 was investigated using Small Baseline Subset Interferometric Synthetic Aperture Radar (SBAS-SAR) technology to process the Advanced Land Observing Satellite (ALOS) and Sentinel-1A SAR datasets, and their cause and the correlation with groundwater level changes and the underground space utilization were discussed. We found that the land subsidence rate in the study area slowed from 2007–2019, and the subsidence area shrank and gradually developed into three relatively independent and isolated subsidence areas primarily. Significant local rebound deformation up to 22 mm/y commenced in the groundwater recharge region during 2015–2019. The magnitude of local rebound was dominated by the rise in groundwater level due to HWTP, whereas tectonic faults and ground fissures control the range of subsidence and the uplift area. The influence of building load on surface deformation became increasingly evident and primarily manifested by slowing the subsidence reduction trend. Additionally, land subsidence caused by the disturbances during the subway construction period was stronger than that in the operational stage. Future land subsidence in Xi’an is predicted to be alleviated overall, and the areas of rebound deformation will continue increasing for a limited time. However, uneven settlement range may extend to the Qujiang and Xixian New District due to the rapid urban construction. Our results could provide a scientific basis for land subsidence hazard mitigation, underground space planning, and groundwater management in Xi’an or similar regions where severe ground subsidence was induced by rapid urbanization.