bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Different Processing Methods on the Millet Polyphenols and Their Anti-diabetic Potential
Authors: Wang Han , Fu Yongxia , Zhao Qingyu , Hou Dianzhi , Yang Xuehao , Bai Shuqun , Diao Xianmin , Xue Yong , Shen Qun
Published: February 2022

Interest in polyphenols has grown due to their beneficial effect on diabetes attenuation. Millets are ancient crops that are rich in polyphenols and used for both food and feed. They are grown worldwide and are adapted to production under dry, hot conditions. The polyphenols found in millets have anti-diabetic properties. However, millet is usually consumed after being processed by heating, germination, fermentation, and other processing methods, which may alter polyphenol content and thus affect their anti-diabetic potential. This mini-review profiles the effects of different processing methods on millet polyphenols and how changes in millet polyphenols affect the hypoglycemic effect of millet. Future studies are needed to compare the anti-diabetes potential of millet polyphenols before and after processing and to explore ways to minimize polyphenol losses and thus maintain their hypoglycemic effect in final products.
Effect of Plant-Derived n-3 Polyunsaturated Fatty Acids on Blood Lipids and Gut Microbiota: A Double-Blind Randomized Controlled Trial
Authors: Liu Hongjie , Li Xiaoqin , Zhu Yalun , Huang Yue , Zhang Qin , Lin Shan , Fang Can , Li Linyan , Lv Yanling , Mei Wenhua , Peng Xiaolin , Yin Jiawei , Liu Liegang
Published: February 2022

Background

Several cardioprotective mechanisms attributed to n-3 polyunsaturated fatty acids (PUFAs) have been widely documented. Significant interest has recently focused on the role of human gut microbiota in metabolic disorders. However, the role of plant-derived n-3 PUFAs on blood lipid profiles is controversial and the effect on gut microbiota is still unclear.

Objectives

We aimed to perform a double-blind randomized controlled trial to test the effect of plant-derived n-3 PUFAs on the blood lipids and gut microbiota of patients with marginal hyperlipidemia.

Methods

According to the inclusion and exclusion criteria, 75 participants with marginal hyperlipidemia were randomly assigned to the intervention group (supplied with n-3 PUFA-enriched plant oil) or control group (supplied with corn oil), respectively, for a 3-month treatment. Participants and assessors were blinded to the allocation. The primary outcomes of the trial were the changes in serum lipid levels. Secondary outcomes were changes in gut microbiota and metabolites. For the primary outcomes, we conducted both an intent-to-treat (ITT) analysis and a per protocol (PP) analysis. For the secondary outcomes, we only conducted the PP analysis among the participants who provided fecal sample.

Results

Fifty-one participants completed the trial. Relative to the control group, the n-3 PUFA supplementation resulted in significant reduction in total cholesterol (TC) levels (−0.43 mmol/L, 95% CI−0.84 to−0.01 mmol/L, P < 0.05). The n-3 PUFA supplementation was also associated with significantly increased relative abundance of Bacteroidetes in phylum level (P < 0.01; false discovery rate (FDR) corrected p = 0.11), and decreased the ratio between Firmicutes and Bacteroidetes (P < 0.05; FDR corrected p = 0.16). At genus level, the intervention of plant derived n-3 PUFAs resulted in a significant decrease in relative abundance of Phascolarctobacterium (P < 0.01; FDR corrected p = 0.18) and Veillonella (P < 0.01; FDR corrected p = 0.18) after the intervention.

Conclusions

Our results demonstrated that plant-derived n-3 PUFAs beneficially affected the serum levels of TC and decreased the ratio between Firmicutes and Bacteroidetes during the 12-week intervention period, which might confer advantageous consequences for lipid metabolism and intestinal health.
Quality of Life After Vestibular Schwannoma Surgery: A Question of Perspective
Authors: Bender Miriam , Tatagiba Marcos , Gharabaghi Alireza
Published: February 2022

Objective

Health-related quality of life (HRQoL) and self-reported outcome measures have a relevant impact on the medical decision-making process. They capture either the current status and allow for multiple prospective evaluations in the course of a treatment or rely on the retrospective comparison of health of patients before and after an intervention to assess its benefit. Importantly, these patient-assessed measures may be influenced by psychological factors. We compared HRQoL and perceived benefit in the course of surgical vestibular schwannoma (VS) treatment, as assessed by the patients from a prospective and retrospective point-of-view, and evaluated the influence of co-morbid depression.

Methods

Within a prospective observational single-center study, forty-three patients with VS were investigated before and after retrosigmoid tumor resection. SF-36, Beck Depression Inventory and patient-assessed clinical symptoms were acquired before surgery and at follow-up. At follow-up, the Glasgow Benefit Inventory (GBI) was acquired as well.

Results

SF-36 scores were significantly lower than the age and sex matched normative data in six and three out of eight categories before and after surgery, respectively. Three categories improved significantly after vs. before surgery; one of them (global health) reached a minimal clinical important difference. In contrast, patients reported predominantly a deterioration, when asked for a retrospective evaluation of the benefit (i.e., GBI). Depression correlated with both SF-36 and GBI, determined dissatisfaction, improved significantly after surgery and was the measure that had the largest impact on HRQoL.

Conclusion

Prospective and retrospective HRQoL measures may lead to different findings and can be confounded by psychological factors.
From Mouse to Human: Cellular Morphometric Subtype Learned From Mouse Mammary Tumors Provides Prognostic Value in Human Breast Cancer
Authors: Chang Hang , Yang Xu , Moore Jade , Liu Xiao-Ping , Jen Kuang-Yu , Snijders Antoine M. , Ma Lin , Chou William , Corchado-Cobos Roberto , García-Sancha Natalia , Mendiburu-Eliçabe Marina , Pérez-Losada Jesus , Barcellos-Hoff Mary Helen , Mao Jian-Hua
Published: February 2022

Mouse models of cancer provide a powerful tool for investigating all aspects of cancer biology. In this study, we used our recently developed machine learning approach to identify the cellular morphometric biomarkers (CMB) from digital images of hematoxylin and eosin (H&E) micrographs of orthotopic Trp53-null mammary tumors (n = 154) and to discover the corresponding cellular morphometric subtypes (CMS). Of the two CMS identified, CMS-2 was significantly associated with shorter survival (p = 0.0084). We then evaluated the learned CMB and corresponding CMS model in MMTV-Erbb2 transgenic mouse mammary tumors (n = 53) in which CMS-2 was significantly correlated with the presence of metastasis (p = 0.004). We next evaluated the mouse CMB and CMS model on The Cancer Genome Atlas breast cancer (TCGA-BRCA) cohort (n = 1017). Kaplan–Meier analysis showed significantly shorter overall survival (OS) of CMS-2 patients compared to CMS-1 patients (p = 0.024) and added significant prognostic value in multi-variable analysis of clinical and molecular factors, namely, age, pathological stage, and PAM50 molecular subtype. Thus, application of CMS to digital images of routine workflow H&E preparations can provide unbiased biological stratification to inform patient care.
Therapeutic Potential of Glutamine Pathway in Lung Cancer
Authors: Tang Enyu , Liu Siyang , Zhang Zhiming , Zhang Rixin , Huang Dejing , Gao Tong , Zhang Tianze , Xu Guangquan
Published: February 2022

Cancer cells tend to obtain the substances needed for their development depending on altering metabolic characteristics. Among the reorganized metabolic pathways, Glutamine pathway, reprogrammed to be involved in the physiological process including energy supply, biosynthesis and redox homeostasis, occupies an irreplaceable role in tumor cells and has become a hot topic in recent years. Lung cancer currently maintains a high morbidity and mortality rate among all types of tumors and has been a health challenge that researchers have longed to overcome. Therefore, this study aimed to clarify the essential role of glutamine pathway played in the metabolism of lung cancer and its potential therapeutic value in the interventions of lung cancer.
Meta-Analysis on the Neutrophil-Lymphocyte Ratio in Rectal Cancer Treated With Preoperative Chemoradiotherapy: Prognostic Value of Pre- and Post-Chemoradiotherapy Neutrophil-Lymphocyte Ratio
Authors: Lee Soo Jin , Kim Kyubo , Park Hae Jin
Published: February 2022

Background

To evaluate the prognostic value of neutrophil-lymphocyte ratio (NLR) in rectal cancer patients treated with preoperative chemoradiotherapy (CRT) and curative surgery.

Methods

A comprehensive search of the EMBASE and PubMed databases was performed to screen studies that compared treatment outcomes according to the pre-CRT and/or post-CRT NLR in patients receiving preoperative CRT and curative surgery for locally advanced rectal cancer. Hazard ratios (HRs) for disease-free survival (DFS) and/or overall survival (OS) were extracted, and a random-effects model was used for pooled analysis.

Results

Totally, 22 retrospective studies comprising 6316 patients were included. Preoperative CRT was administered with concurrent chemotherapy (mostly fluoropyrimidine-based regimens). The elevated pre-CRT NLR was significantly associated with an increased risk of recurrence (HR, 1.54; 95% confidence interval [CI], 1.31-1.81) and death (HR, 2.14; 95% CI, 1.61-2.84). Post-CRT NLR was reported in only 3 of 22 studies, and the correlation was not statistically significant for recurrence (HR, 1.44; 95% CI, 0.86-2.41) or death (HR, 2.38; 95% CI, 0.94-6.07).

Conclusions

Elevated pre-CRT NRL, but not post-CRT NRL, is associated with inferior DFS and OS. Further studies are needed to confirm the prognostic value of NLR in rectal cancer patients receiving preoperative CRT.
Impact of Different Synchrotron Flattop Operation Modes on 4D Dosimetric Uncertainties for Scanned Carbon-Ion Beam Delivery
Authors: He Pengbo , Li Qiang
Published: February 2022

Purpose

The characteristic of pulsed beam delivery for synchrotron-based carbon-ion radiotherapy has led to the emergence of many scanning scenarios in order to improve the treatment efficiency and accuracy of moving target volume. Here, we aim to evaluate a novel breathing guidance motion mitigation performance under different synchrotron flattop operation modes in carbon-ion radiotherapy.

Methods

With the use of twelve 4DCT datasets of lung cancer patients who had been treated with respiratory-gated carbon-ion pencil beam therapy, range-adapted internal target volume (raITV) plans were optimized. Under the fixed flattop with single-energy and extended flattop with multi-energy synchrotron operation modes, the 4D treatments with breathing guidance and free breathing-based gated phase-controlled rescanning (PCR) beam delivery were simulated. Dose metrics (D95 and D5–D95 in clinical target volume (CTV)) and treatment time of the resulting 4D plans were compared.

Results

The two synchrotron operation modes provided different scanning dynamics. For the free breathing-based PCR method delivered in the extended flattop operation mode, the averaged CTV-D95 values were 90.4% ± 3.7%, 95.4% ± 1.7%, 96.9% ± 1.5%, 97.2% ± 1.5%, and 97.3% ± 1.5% for the 1-scanning, 2-PCR, 4-PCR, 6-PCR, and 8-PCR, respectively. For the breathing guidance-based PCR method delivered in the extended flattop mode, these values were 89.1% ± 4.0%, 97.0% ± 1.4%, 98.2% ± 0.7%, 98.6% ± 0.7%, and 98.9% ± 0.7%, respectively. However, CTV-D95 significantly increased to 98.5% ± 1.0% even with just 1-scanning breathing guidance-based fixed flattop operation mode (p < 0.01). Moreover, there was no significant difference in treatment time among the three technical combinations (p > 0.15).

Conclusions

The combination of the breathing guidance and PCR methods should be an alternative way for motion mitigation for the fixed flattop synchrotron operation mode. The target dose coverage and homogeneity could be further improved by the combination of the breathing guidance and PCR methods than the traditional PCR-only technology for the extended flattop synchrotron operation mode.
Neonatal Mortality and Associated Factors Among Neonates Admitted to the Neonatal Intensive Care Unit of Dil Chora Referral Hospital, Dire Dawa City, Ethiopia, 2021: A Facility-Based Study
Authors: Thomas Gelila , Demena Melake , Hawulte Behailu , Eyeberu Addis , Heluf Helina , Tamiru Dawit
Published: February 2022

Background

Despite the incredible progress made in decreasing under-five mortality, neonatal mortality remains the main and slowly advancing contributor. Though all efforts were made to decline the death of a newborn, current information showed that newborn death is unaverted and not a fastened agenda. This study aimed to assess neonatal mortality and its determinants among neonates admitted at the Dil Chora Hospital in Dire Dawa City.

Methods

Facility-based cross-sectional study design was employed. A total of 376 newborns were selected systematically from neonates admitted to the NICU of the Dil Chora Referral Hospital from June 1, 2017 to December 31, 2020. Data were collected from medical records using a standard extraction checklist. The data were entered into Epi-data version 3.1 and then exported into SPSS version 24 for statistical analysis. Bivariate and multivariate analyses were employed to identify the association between independent variables and neonatal death.

Result

The prevalence of neonatal death was 11.4% (95% CI: 9.44, 13.36). The majority of 37 (86.05%) of the neonates died within 7 days of life. The most common causes of admission included perinatal asphyxia (55.3%), hypoglycemia (21.5%), and hypothermia. Nearly half (40.4%) of the mothers of newborns experience index pregnancy complications, premature rupture of the membrane (AOR = 5.79, 95% CI: 2.08, 16.1), birth weight <2,500 g (AOR = 3.96, 95% CI: 1.56. 10.06), hypothermia (AOR = 2.54, 95% CI: 1.1, 6.02), index pregnancy complications (AOR = 4.79, 95% CI: 1.92, 11.91), and induced labor (AOR = 4.45, 95% CI: 1.53, 12.94), which were significantly associated with neonatal mortality.

Conclusion

The prevalence of neonatal mortality was high compared with the national target. Premature rupture of the membrane, birth weight <2,500 g, hypothermia, index pregnancy complications, and induced labor were significantly associated with neonatal mortality. The majority of neonatal deaths are due to complications arising from pregnancy, labor, and delivery, and lack of quality of care at the neonatal intensive care unit. Cultivating and increasing the utilization of antenatal care services, quality of care at delivery, and the neonatal intensive care unit could avert those deaths.
The Neuroprotective Effects of Coreopsis tinctoria and Its Mechanism: Interpretation of Network Pharmacological and Experimental Data
Authors: Ma Pei , Zhang Rong , Xu Lijia , Liu Haibo , Xiao Peigen
Published: February 2022

Background:Coreopsis tinctoria Nutt. (CT), an annual herb in the genus Coreopsis, is an important traditional medicine to be used for antidiabetes and antioxidation.

Objective: The antioxidant compounds from CT may affect mitochondrial function and apoptosis, which in turn may affect related diseases. The aim of this study was to explore the potential molecular mechanism and new therapeutic opportunities of CT based on network pharmacology.

Methods: A network pharmacology-based method, which combined data collection, drug-likeness filtering, target prediction, disease prediction, and network analysis, was used to decipher the potential targets and new therapeutic opportunities of CT. The potential molecular mechanism and pathway were explored through Gene Ontology (GO) and KEGG analyses. Then MPTP-induced SH-SY5Y cell model was applied to evaluate the neuroprotective effects and key targets.

Results: There were 1,011 targets predicted for 110 compounds. Most targets were regulated by flavones, phenylpropanoids, and phenols and had synergistic effects on memory impairment, pancreatic neoplasm, fatty liver disease, and so on. The compounds–targets–diseases network identified TNF, PTGS2, VEGFA, BCL2, HIF1A, MMP9, PIK3CG, ALDH2, AKT1, and EGFR as key targets. The GO and KEGG analyses revealed that the cell death pathway, mitochondrial energy metabolism, and PI3K-AKT signal pathway were the main pathways. CT showed neuroprotective effects via regulating gene and protein expression levels of key targets in an in vitro model.

Conclusion: CT had potential neuroprotective effects by targeting multiple targets related with apoptosis, which were affected by the BCL-2 and AKT signaling pathways. This study provided a theoretical basis for the research of neuroprotective effects of CT.
Application of Transgenic Zebrafish Models for Studying the Effects of Estrogenic Endocrine Disrupting Chemicals on Embryonic Brain Development
Authors: Takesono Aya , Kudoh Tetsuhiro , Tyler Charles R.
Published: February 2022

Endocrine disrupting chemicals (EDCs) are environmental pollutants that mimic hormones and/or disrupt their function. Estrogenic EDCs (eEDCs) interfere with endogenous estrogen signalling pathway(s) and laboratory animal and human epidemiological studies have provided evidence for a causal link between exposure to them during embryonic/early life and neurological impairments. However, our understanding of the molecular and cellular mechanism(s) underlying eEDCs exposure effects on brain development, tissue architecture and function and behaviour are limited. Transgenic (TG) zebrafish models offer new approach methodologies (NAMs) to help identify the modes of action (MoAs) of EDCs and their associated impacts on tissue development and function. Estrogen biosensor TG zebrafish models have been applied to study eEDC interactions and resulting transcriptional activation (viaa fluorescent reporter expression) across the entire body of the developing zebrafish embryo, including in real time. These estrogen biosensor TG zebrafish models are starting to deepen our understanding of the spatiotemporal actions of eEDCs and their resulting impacts on neurological development, brain function and behaviour. In this review, we first investigate the links between early life exposure to eEDCs and neurodevelopmental alterations in model organisms (rodents and zebrafish) and humans. We then present examples of the application of estrogen biosensor and other TG zebrafish models for elucidating the mechanism(s) underlying neurodevelopmental toxicities of eEDCs. In particular we illustrate the utility of combining estrogen biosensor zebrafish models with other TG zebrafish models for understanding the effects of eEDCs on the brain, spanning cellular processes, brain circuitry, neurophysiology and behaviour. Finally, we discuss the future prospects of TG zebrafish models as experimental models for studying more complex scenarios for exposure to contaminant mixtures on neurological development and function.
Exploring the Anti-Pulmonary Fibrosis Mechanism of Jingyin Granule by Network Pharmacology Strategy
Authors: Zhu De-wei , Yu Qun , Jiang Mei-fang , Wang Dan-dan , Shen Yun-hui
Published: February 2022

Pulmonary fibrosis (PF) is a clinically common disease caused by many factors, which will lead to lung function decline and even respiratory failure. Jingyin granule has been confirmed to have anti-inflammatory and antiviral effects by former studies, and has been recommended for combating H1N1 influenza A virus (H1N1) infection and Coronavirus disease 2019 (COVID-19) in China. At present, studies have shown that patients with severe COVID-19 infection developed lung fibrotic lesions. Although Jingyin granule can improve symptoms in COVID-19 patients, no study has yet reported whether it can attenuate the process of PF. Here, we explored the underlying mechanism of Jingyin granule against PF by network pharmacology combined with in vitro experimental validation. In the present study, the active ingredients as well as the corresponding action targets of Jingyin granule were firstly collected by TCMSP and literature data, and the disease target genes of PF were retrieved by disease database. Then, the common targets were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, and then a PPI network and an ingredient–target network were constructed. Next, UPLC-MS was used to isolate and identify selected representative components in Jingyin granule. Finally, LPS was used to induce the A549 cell fibrosis model to verify the anti-PF effect of Jingyin granule in vitro. Our results indicated that STAT3, JUN, RELA, MAPK3, TNF, MAPK1, IL-6, and AKT1 were core targets of action and bound with good affinity to selected components, and Jingyin granule may alleviate PF progression by Janus kinase 2/signal transducers and activators of transcription (JAK2/STAT3), the mammalian nuclear factor-κB (NF-κB), the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), tumor necrosis factor (TNF), and the extracellular signal-regulated kinases 1 and 2 (ERK1/2) signaling pathways. Overall, these results provide future therapeutic strategies into the mechanism study of Jingyin granule on PF.
Carrier Doping Physics of Rare Earth Perovskite Nickelates RENiO3
Authors: Li Jiarui , Ramanathan Shriram , Comin Riccardo
Published: February 2022

The family of rare earth (RE) nickelate perovskites RENiO3 has emerged over the past two decades as an important platform for quantum matter physics and advanced applications. The parent compounds from this family are strongly correlated insulators or metals, in most cases with long-range spin order. In the past few years, carrier doping has been achieved using different approaches and has been proven to be a powerful tuning parameter for the microscopic properties and collective macroscopic states in RENiO3 compounds. In particular, a series of recent studies has shown that carrier doping can be responsible for dramatic but reversible changes in the long-range electronic and magnetic properties, underscoring the potential for use of nickelates in advanced functional devices. In this review, we discuss the recent advancements in our description, understanding and application of electron-doped rare earth nickelates. We conclude with a discussion of the developments and outlook for harnessing the quantum functional properties of nickelates in novel devices for sensing and neuromorphic computation.
Spin-Torque Oscillator Based on Magnetic Domain and Meron
Authors: Lv Gang , Zhang Hong , Cao Xuecheng , Gao Feng , Li Guihua , Sun Fengwei , Hou Zhiwei , Liu Yaowen
Published: February 2022

In this work, micromagnetic simulations demonstrate that a steady oscillation mode accompanied by magnetic domain splitting and the creation and annihilation of meron can be excited by spin-polarized currents. It is found that the in-plane magnetic anisotropy and Dzyaloshinskii-Moriya interaction (DMI) have a greater influence on the oscillation frequency. The oscillation frequency can vary from 3 GHz to 31 GHz by controlling anisotropy strength under a fixed current density. By changing DMI strength, the oscillation frequency varies from 9 to 13.6 GHz and from 29.7 to 37 GHz. Compared with ferromagnetic skyrmion-based spin-torque oscillators (STOs), the STOs based on this magnetic domain and meron further increase the oscillation frequency. Our results may provide theoretical support for the research and development of future high-frequency STOs.
Assessment of the Psychophysiological State of Female Operators Under Simulated Microgravity
Authors: Lebedeva Svetlana , Shved Dmitry , Savinkina Alexandra
Published: February 2022

The article describes methods of non-verbal speech characteristics analysis used to determine psychophysiological state of female subjects under simulated microgravity conditions (“dry” immersion, DI), as well as the results of the study. A number of indicators of the acute period of adaptation to microgravity conditions was described. The acute adaptation period in female subjects began earlier (evening of the 1st day of DI) and ended faster than in male ones in previous studies (2nd day of DI). This was indicated by a decrease in the level of state anxiety (STAI, p < 0,05) and depression-dejection [Profile of Mood States (POMS), p < 0,05], as well as a decrease in pitch (p < 0,05) and voice intensity (p < 0,05). In addition, women, apparently, used the “freeze” coping strategy – the proportion of neutral facial expressions on the most intense days of the experiment was at maximum. The subjects in this experiment assessed their feelings and emotions better, giving more accurate answers in self-assessment questionnaires, but at the same time tried to look and sound as calm and confident as possible, controlling their expressions. Same trends in the subjects’ cognitive performance were identified as in similar experimental conditions earlier: the subjects’ psychophysiological excitement corresponded to better performance in sensorimotor tasks. The difference was in the speed of mathematical computation: women in the present study performed the computation faster on the same days when they made fewer pauses in speech, while in men in previous experiments this relationship was inverse.
Brain Oxygen Extraction and Metabolism in Pediatric Patients With Sickle Cell Disease: Comparison of Four Calibration Models
Authors: Lin Zixuan , McIntyre Tiffany , Jiang Dengrong , Cannon Alicia , Liu Peiying , Tekes Aylin , Casella James F. , Slifer Keith , Lu Hanzhang , Lance Eboni
Published: February 2022

Sickle cell disease (SCD) is an inherited hemoglobinopathy with an increased risk of neurological complications. Due to anemia and other factors related to the underlying hemoglobinopathy, cerebral blood flow (CBF) increases as compensation; however, the nature of alterations in oxygen extraction fraction (OEF) and cerebral metabolic rate of oxygen (CMRO2) in SCD remains controversial, largely attributed to the different calibration models. In addition, limited studies have been done to investigate oxygen metabolism in pediatric patients. Thus, this study used a non-invasive T2-based MR oximetry, T2-Relaxation-Under-Spin-Tagging (TRUST) MRI, to measure oxygen homeostasis in pediatric patients with SCD using four different calibration models and examined its relationship to hematological measures. It was found that, compared with controls, SCD patients showed an increased CBF, unchanged total oxygen delivery and increased venous blood T2. The results of OEF and CMRO2 were dependent on the calibration models used. When using sickle-specific, hemoglobin S (HbS) level-dependent calibration, there was a decreased OEF and CMRO2, while the bovine model showed an opposite result. OEF and CMRO2 were also associated with hemoglobin and HbS level; the direction of the relationship was again dependent on the model. Future studies with in vivo calibration are needed to provide more accurate information on the T2-Yv relationship.
Epigenetic Regulation of TET1-SP1 During Spermatogonia Self-Renewal and Proliferation
Authors: Liu Lingling , Wang Jin , Wang Shenghua , Wang Mudi , Chen Yuanhua , Zheng Liming
Published: February 2022

Spermatogonia are the source of spermatogenic waves. Abnormal spermatogonia can cause ab-normal spermatogenic waves, which manifest as spermatogenic disorders such as oligospermia, hypospermia, and azoospermia. Among them, the self-renewal of spermatogonia serves as the basis for maintaining the process of spermatogenesis, and the closely regulated balance between self-renewal and differentiation of spermatogonia can maintain the continuous production of spermatozoa. Tet methylcytosine dioxygenase 1(TET1) is an important epitope modifying enzyme that catalyzes the conversion of 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC), thereby causing the methylation of specific genes site hydroxylation, enabling the DNA de-methylation process, and regulating gene expression. However, the hydroxymethylation sites at which TET1 acts specifically and the mechanisms of interaction affecting key differential genes are not clear. In the present study, we provide evidence that the expression of PLZF, a marker gene for spermatogonia self-renewal, was significantly elevated in the TET1 overexpression group, while the expression of PCNA, a proliferation-related marker gene, was also elevated at the mRNA level. Significant differential expression of SP1 was found by sequencing. SP1 expression was increased at both mRNA level and protein level after TET1 overexpression, while differential gene DAXX expression was downregulated at protein level, while the expression of its reciprocal protein P53 was upregulated. In conclusion, our results suggest that TET1 overexpression causes changes in the expression of SP1, DAXX and other genes, and that there is a certain antagonistic effect between SP1 and DAXX, which eventually reaches a dynamic balance to maintain the self-renewal state of spermatogonia for sustained sperm production. These findings may contribute to the understanding of male reproductive system disorders.
An Efficient Genetic Transformation and CRISPR/Cas9-Based Genome Editing System for Moso Bamboo (Phyllostachys edulis)
Authors: Huang Biyun , Zhuo Renying , Fan Huijin , Wang Yujun , Xu Jing , Jin Kangming , Qiao Guirong
Published: February 2022

Moso bamboo (Phyllostachys edulis) is the most important monopodial bamboo species worldwide. Without a genetic transformation system, it is difficult to verify the functions of genes controlling important traits and conduct molecular breeding in moso bamboo. Here, we established a plant regeneration system from immature embryos. Calli were induced on MS medium added 4–6 mg⋅L–1 2,4-dichlorophenoxyacetic acid (2,4-D) with high efficiency (>60%). A plant growth regulator combination of 0.5 mg⋅L–1 1-naphthylacetic acid (NAA), 2.0 mg⋅L–1 6-benzylaminopurine (BAP), and 3.0 mg⋅L–1 zeatin (ZT) was suitable for shoot differentiation, and the shoot induction frequency was increased to 43% after 0.5 mg⋅L–1 abscisic acid (ABA) pretreatment. An effective antibiotic screening concentration was determined by hygromycin sensitivity test. We further optimized the Agrobacterium concentration and added vacuum infiltration for infection, which improves the transient expression efficiency. A genetic transformation system was established for the first time in moso bamboo, with the transformation efficiency of approximately 5%. To optimize genome editing, two endogenous U3 small nuclear RNA (snRNA) promoters were isolated and used to drive small guide RNA (sgRNA) expression. The results showed that the PeU3.1 promoter exhibited higher efficiency, and it was used for subsequent genome editing. Finally, homozygous pds1pds2 mutants were obtained by an efficient CRISPR/Cas9 genome-editing system. These technical systems will be conducive to gene functional validation and accelerate the molecular breeding process of moso bamboo.
COVID-19 Repercussions: Office and Residential Emissions in Pakistan
Authors: Rehmani Mahmood , Arshad Madiha , Khokhar Munnawar Naz , Anwer Naveed , Adnan Mohammad , Naveed Rana Tahir , Irshad Huda
Published: February 2022

The purpose of this study is to find empirical evidence on whether work from home or residential emissions reduces office emissions. Based on existing research the study supports that there are short-term effects on office emissions, i.e., carbon emissions do not outshine the long-term effects. The shift from offices to working from home due to COVID-19 regulations meant more people operating from home as maintaining their position in the market was crucial. The potential research area is to understand how this would affect energy usage and carbon emissions. This study has used a before and after mixed approach to collect data from 301 working-from-home employees and 348 top managers who are responsible for monitoring the employees in a work from home setting. Convenience sampling helped collect responses in a timely manner as offices were not allowing visitors and collecting data in person was difficult, so online surveys were conducted. Work from home reduced usage of office equipment, transportation, pollution, etc. The air quality improved considerably but our findings show that the low emissions were only short-lived. This was not a long-term scenario as organizations kept practicing their operations even at home and the emissions stayed in the environment. Future suggestions and implications are also provided. The results give new insights to researchers in the field of sustainability and the environment.
The Mediating Effect of Bicultural Self-Efficacy on Acculturation and Career Decision-Making Self-Efficacy for International Students in South Korea
Authors: Jin Yuan Ying , Ahn Sungsik , Lee Sang Min
Published: February 2022

The population of international students in South Korea is growing. During the career development phase, international students face unique challenges related to their bicultural identity and acculturation experiences. The present study examined the role of bicultural self-efficacy on mediating the relationship between acculturation and career decision-making self-efficacy for international students in South Korea. Responses from 120 international students in South Korea were analyzed using structural equation modeling. The results showed that bicultural self-efficacy fully mediated the relationship between acculturation to mainstream culture and career decision-making self-efficacy but did not mediate the relationship between acculturation to heritage culture and career decision-making self-efficacy. The implications for effective educational intervention for international college students’ career development were discussed based on the results.
Does Playing Apart Really Bring Us Together? Investigating the Link Between Perceived Loneliness and the Use of Video Games During a Period of Social Distancing
Authors: Nebel Steve , Ninaus Manuel
Published: February 2022

During the COVID-19 pandemic, several countries implemented social distancing measures to contain virus transmission. However, these vital safety measures have the potential to impair mental health or wellbeing, for instance, from increased perceived loneliness. Playing social video games may offer a way to continue to socialize while adhering to social distancing measures. To examine this issue further, the present online survey investigated social gaming during the pandemic and its association to perceived loneliness within a German-speaking sample. Results indicated a small positive correlation between general gaming frequency and perceived loneliness. Detailed analysis revealed a negative association between perceived loneliness and increased social forms of video gaming. Specifically, gamers with a higher social motive for gaming perceived less loneliness, but gamers with a dominant escape motive demonstrated a positive link to perceived loneliness. The use of social gaming in times of social distancing seems to play a small but significant factor in perceived loneliness compared to other demographical data. The findings are discussed with respect to methodological limitations, effect sizes, and sample characteristics. The results enrich the current knowledge on video gaming and its link to social wellbeing and provide a more nuanced picture than simplistic investigations of screen time.
Non-symbolic Ratio Reasoning in Kindergarteners: Underlying Unidimensional Heuristics and Relations With Math Abilities
Authors: Muñez David , Bull Rebecca , Cheung Pierina , Orrantia Josetxu
Published: February 2022

Although it is thought that young children focus on the magnitude of the target dimension across ratio sets during binary comparison of ratios, it is unknown whether this is the default approach to ratio reasoning, or if such approach varies across representation formats (discrete entities and continuous amounts) that naturally afford different opportunities to process the dimensions in each ratio set. In the current study, 132 kindergarteners (Mage = 68 months, SD = 3.5, range = 62–75 months) performed binary comparisons of ratios with discrete and continuous representations. Results from a linear mixed model revealed that children followed an additive strategy to ratio reasoning—i.e., they focused on the magnitude of the target dimension across ratio sets as well as on the absolute magnitude of the ratio set. This approach did not vary substantially across representation formats. Results also showed an association between ratio reasoning and children’s math problem-solving abilities; children with better math abilities performed better on ratio reasoning tasks and processed additional dimensions across ratio sets. Findings are discussed in terms of the processes that underlie ratio reasoning and add to the extant debate on whether true ratio reasoning is observed in young children.
The Impact of Positive Mood and Future Outlook on English as a Foreign Language Students’ Academic Self-Concept
Authors: Zhang Jingsheng
Published: February 2022

Due to the fact that English as a foreign language (EFL) students’ academic self-concept is of high importance for their academic motivation, academic achievement, and L2 success, many investigations have been done to uncover the personal and interpersonal factors that may contribute to students’ academic self-concept. Yet, the emotional and psychological factors have rarely been studied. In addition, no empirical and review study has been carried out to probe into the impact of positive mood and future outlook on EFL students’ academic self-concept. Accordingly, the current review study seeks to illustrate the effects of positive mood and future outlook on EFL students’ academic self-concept. Building upon the principles of positive psychology (PP), the favorable effects of positive mood and future outlook on EFL students’ academic self-concept were thoroughly explained. The educational implications and suggestions for future research are also highlighted.
Social Context, Self-Efficacy, and Patient-Centered Service Behavior of Medical Professionals: The Moderating Role of Achievement Motivation
Authors: Huang Xianhong , Wang Cong , Hua Le , Gao Yuan , Zhou Siyu , Wang Xiaohe
Published: February 2022

Patient-centered services are high-value, high-quality medical services that can improve patient satisfaction and safety. However, little is known about their driving mechanisms. This study examined whether external (social context) and internal (self-efficacy) motivation affects medical professionals' patient-centered service behavior, and explored the moderating role of medical professionals' achievement motivation. A cross-sectional survey was conducted with medical professionals at public hospitals in Hangzhou. Descriptive statistics, hierarchical linear regression analysis, and structural equation modeling were used to analyze the data. The final analysis included 1,612 medical professionals. Results indicated that perceived social context and self-efficacy had direct and positive effects (β = 0.578, β = 0.269) on medical professionals' patient-centered service behavior. Social context also indirectly influenced medical professionals' patient-centered service behavior mediated by self-efficacy (β = 0.149). Additionally, achievement motivation played a moderating role (β = −0.037) between the social context and self-efficacy. From the results it can be deduced that an excellent social environment and strong self-efficacy can promote the patient-centered service motivation of medical professionals. This promoting effect is even more significant under the moderating effect of high achievement motivation. Governments, health administrative departments, and hospital management should use internal and external motivation factors to promote medical professionals' patient-centered service behavior. Along with formulating relevant laws and regulations, efforts should also be made to guide medical professionals to improve their self-efficacy and achievement motivation, thereby encouraging patient-centered medical service behavior.
How Does It Feel to Have One's Psychiatric Diagnosis Altered? Exploring Lived Experiences of Diagnostic Shifts in Adult Mental Healthcare
Authors: O'Connor Cliodhna , Seery Christina , Young Claire
Published: February 2022

Background

Though the socio-emotional significance of psychiatric diagnoses and the frequency of transitions between diagnostic classifications are widely acknowledged, minimal research reveals how “diagnostic shifts” are subjectively experienced by psychiatric service-users.

Aim

This study investigated how adult service-users make sense of diagnostic shifts and their impacts on one's life.

Methods

Twenty-seven people with self-reported experiences of diagnostic shifts opted into this qualitative study. Virtual narrative interviews invited participants to share their “diagnosis stories.” Interview transcripts were analyzed using narrative thematic analysis to identify common and divergent experiences across participants.

Results

Diverse experiences of diagnostic shifts were related: diagnostic shifts could both promote and undermine clinical trust, therapeutic engagement and self-understanding. The analysis suggested that shared and divergent experiences could be attributed to two dimensions of narratives: participants'Interpretations of Diagnostic ShiftsandDiagnosis-Specific Factors. Regarding the former, analysis produced a typology of three possible interpretations of diagnostic shifts, which were linked with consistently different antecedents, experiences and consequences. The latter dimension captured how experiences of diagnostic shifts also hinged on the unique meanings ascribed to the specific diagnoses gained and lost, particularly in relation to their perceived severity, stigma, personal associations, and related communities.

Conclusions

Findings revealed how diagnostic shifts can be experienced as both traumatic and life-enhancing, depending on their social and subjective context. Understanding the range and predictors of variable experiences of diagnostic shifts is vital for sensitive clinical practice and communication.
Association Between Graphic Health Warning Labels on Cigarette Packs and Smoking Cessation Attempts in Korean Adolescent Smokers: A Cross-Sectional Study
Authors: Joo Hye Jin , Joo Jae Hong , Kim Seung Hoon , Park Eun-Cheol , Jang Sung-In
Published: February 2022

Graphic health warning labels on cigarette packs inform smokers about the health risks associated with tobacco smoking. Adolescents are generally the main targets to influence by graphic health warning labels. This study investigated the association between graphic health warning labels on cigarette packs and attempts to quit smoking in South Korean adolescents. This cross-sectional study used data from the 2017 to 2019 Korea Youth Risk Behavior Web-based Survey, using multiple logistic regression for the analysis. The study population comprised 11,142 adolescents aged 12–18 years. The outcome variable was attempts to quit smoking among adolescent smokers who had seen graphic health warning labels. Attempts to quit smoking were higher among adolescent smokers who had seen graphic health warning labels compared to those who had not {boys, odds ratio (OR) = 1.72 [95% confidence interval (CI), 1.48–2.00]; girls, OR = 1.74 (95% CI, 1.33–2.28)}. The correlation was greater for adolescents who thought about the harm of smoking [boys, OR = 1.86 (95% CI, 1.60–2.16); girls, OR = 1.85 (95% CI, 1.41–2.43)] and the willingness to quit [boys, OR = 2.03 (95% CI, 1.74–2.36); girls, OR = 2.04 (95% CI, 1.55–2.68)] after seeing graphic health warning labels. Our findings indicate that graphic health warning labels on cigarette packs have the potential to lower smoking intentions of adolescents. We suggest that the use of graphic health warning labels is an effective policy-related intervention to reduce smoking in South Korean adolescents.
Targeting TNF-α for COVID-19: Recent Advanced and Controversies
Authors: Guo Yi , Hu Ke , Li Yuxuan , Lu Chanjun , Ling Ken , Cai Chuanqi , Wang Weici , Ye Dawei
Published: February 2022

Recent advances in the pathophysiologic understanding of coronavirus disease 2019 (COVID-19) suggests that cytokine release syndrome (CRS) has an association with the severity of disease, which is characterized by increased tumor necrosis factor α (TNF-α), interleukin (IL)-6, IL-2, IL-7, and IL-10. Hence, managing CRS has been recommended for rescuing severe COVID-19 patients. TNF-α, one of the pro-inflammatory cytokines commonly upregulated in acute lung injury, triggers CRS and facilitates SARS-CoV-2 interaction with angiotensin-converting enzyme 2 (ACE2). TNF-α inhibitors, therefore, may serve as an effective therapeutic strategy for attenuating disease progression in severe SARS-CoV-2 infection. Below, we review the possibilities and challenges of targeting the TNF-α pathway in COVID-19 treatment.
Roadmap for Sex-Responsive Influenza and COVID-19 Vaccine Research in Older Adults
Authors: Shapiro Janna R. , Morgan Rosemary , Leng Sean X. , Klein Sabra L.
Published: February 2022

Sex differences in the immune system are dynamic throughout the lifespan and contribute to heterogeneity in the risk of infectious diseases and the response to vaccination in older adults. The importance of the intersection between sex and age in immunity to viral respiratory diseases is clearly demonstrated by the increased prevalence and severity of influenza and COVID-19 in older males compared to older females. Despite sex and age biases in the epidemiology and clinical manifestations of disease, these host factors are often ignored in vaccine research. Here, we review sex differences in the immunogenicity, effectiveness, and safety of the influenza and COVID-19 vaccines in older adults and the impact of sex-specific effects of age-related factors, including chronological age, frailty, and the presence of comorbidities. While a female bias in immunity to influenza vaccines has been consistently reported, understanding of sex differences in the response to COVID-19 vaccines in older adults is incomplete due to small sample sizes and failure to disaggregate clinical trial data by both sex and age. For both vaccines, a major gap in the literature is apparent, whereby very few studies investigate sex-specific effects of aging, frailty, or multimorbidity. By providing a roadmap for sex-responsive vaccine research, beyond influenza and COVID-19, we can leverage the heterogeneity in immunity among older adults to provide better protection against vaccine-preventable diseases.
Regulating Ruminative Web Browsing Based on the Counterbalance Modeling Approach
Authors: Morita Junya , Pitakchokchai Thanakit , Raj Giri Basanta , Yamamoto Yusuke , Yuhashi Hiroyasu , Koguchi Teppei
Published: February 2022

Even though the web environment facilitates our daily life, emotional problems caused by its incompatibility with human cognition are becoming increasingly serious. To alleviate negative emotions during web use, we developed a browser extension that presents memorized product images to users in the form of web advertisements. This system utilizes the cognitive architecture Adaptive Control of Thought-Rational (ACT-R) as a model of human memory and emotion. A heart rate sensor attached to the user modulates the ACT-R model parameters, and the emotional states represented by the model are synchronized (following the chameleon effect) or counterbalanced (following the homeostasis regulation) with the physiological state of the user. An experiment demonstrates that the counterbalanced model suppresses negative ruminative web browsing. The authors claim that this approach, utilizing a cognitive model, is advantageous in terms of explainability.