bluuebunny/crossref_metadata_2025 huggingface dataset:
Full-Length Transcriptome Analysis of Four Different Tissues of Cephalotaxus oliveri
Authors: He Ziqing , Su Yingjuan , Wang Ting
Published: January 2021

Cephalotaxus oliveri is a tertiary relict conifer endemic to China, regarded as a national second-level protected plant in China. This species has experienced severe changes in temperature and precipitation in the past millions of years, adapting well to harsh environments. In view of global climate change and its endangered conditions, it is crucial to study how it responds to changes in temperature and precipitation for its conservation work. In this study, single-molecule real-time (SMRT) sequencing and Illumina RNA sequencing were combined to generate the complete transcriptome of C. oliveri. Using the RNA-seq data to correct the SMRT sequencing data, the four tissues obtained 63,831 (root), 58,108 (stem), 33,013 (leaf) and 62,436 (male cone) full-length unigenes, with a N50 length of 2523, 3480, 3181, and 3267 bp, respectively. Additionally, 35,887, 11,306, 36,422, and 25,439 SSRs were detected for the male cone, leaf, root, and stem, respectively. The number of long non-coding RNAs predicted from the root was the largest (11,113), and the other tissues were 3408 (stem), 3193 (leaf), and 3107 (male cone), respectively. Functional annotation and enrichment analysis of tissue-specific expressed genes revealed the special roles in response to environmental stress and adaptability in the different four tissues. We also characterized the gene families and pathways related to abiotic factors. This work provides a comprehensive transcriptome resource for C. oliveri, and this resource will facilitate further studies on the functional genomics and adaptive evolution of C. oliveri.
A New Mouse Model of Chronic Myocarditis Induced by Recombinant Bacille Calmette–Guèrin Expressing a T-Cell Epitope of Cardiac Myosin Heavy Chain-α
Authors: Tajiri Kazuko , Imanaka-Yoshida Kyoko , Tsujimura Yusuke , Matsuo Kazuhiro , Hiroe Michiaki , Aonuma Kazutaka , Ieda Masaki , Yasutomi Yasuhiro
Published: January 2021

Dilated cardiomyopathy (DCM) is a potentially lethal disorder characterized by progressive impairment of cardiac function. Chronic myocarditis has long been hypothesized to be one of the causes of DCM. However, owing to the lack of suitable animal models of chronic myocarditis, its pathophysiology remains unclear. Here, we report a novel mouse model of chronic myocarditis induced by recombinant bacille Calmette-Guérin (rBCG) expressing a CD4+ T-cell epitope of cardiac myosin heavy chain-α (rBCG-MyHCα). Mice immunized with rBCG-MyHCα developed chronic myocarditis, and echocardiography revealed dilation and impaired contraction of ventricles, similar to those observed in human DCM. In the heart, CD62L−CD4+ T cells were increased and produced significant amounts of IFN-γ and IL-17 in response to cardiac myosin. Adoptive transfer of CD62L−CD4+ T cells induced myocarditis in the recipient mice, which indicated that CD62L−CD4+ T cells were the effector cells in this model. rBCG-MyHCα-infected dendritic cells produced proinflammatory cytokines and induced MyHCα-specific T-cell proliferation and Th1 and Th17 polarization. This novel chronic myocarditis mouse model may allow the identification of the central pathophysiological and immunological processes involved in the progression to DCM.
Inhibition of Orexin/Hypocretin Neurons Ameliorates Elevated Physical Activity and Energy Expenditure in the A53T Mouse Model of Parkinson’s Disease
Authors: Stanojlovic Milos , Pallais Jean Pierre , Kotz Catherine M.
Published: January 2021

Aside from the classical motor symptoms, Parkinson’s disease also has various non-classical symptoms. Interestingly, orexin neurons, involved in the regulation of exploratory locomotion, spontaneous physical activity, and energy expenditure, are affected in Parkinson’s. In this study, we hypothesized that Parkinson’s-disease-associated pathology affects orexin neurons and therefore impairs functions they regulate. To test this, we used a transgenic animal model of Parkinson’s, the A53T mouse. We measured body composition, exploratory locomotion, spontaneous physical activity, and energy expenditure. Further, we assessed alpha-synuclein accumulation, inflammation, and astrogliosis. Finally, we hypothesized that chemogenetic inhibition of orexin neurons would ameliorate observed impairments in the A53T mice. We showed that aging in A53T mice was accompanied by reductions in fat mass and increases in exploratory locomotion, spontaneous physical activity, and energy expenditure. We detected the presence of alpha-synuclein accumulations in orexin neurons, increased astrogliosis, and microglial activation. Moreover, loss of inhibitory pre-synaptic terminals and a reduced number of orexin cells were observed in A53T mice. As hypothesized, this chemogenetic intervention mitigated the behavioral disturbances induced by Parkinson’s disease pathology. This study implicates the involvement of orexin in early Parkinson’s-disease-associated impairment of hypothalamic-regulated physiological functions and highlights the importance of orexin neurons in Parkinson’s disease symptomology.
Lipid Raft Association Stabilizes VEGF Receptor 2 in Endothelial Cells
Authors: Zabroski Ibukunoluwapo O. , Nugent Matthew A.
Published: January 2021

The binding of vascular endothelial growth factor A (VEGF) to VEGF receptor-2 (VEGFR-2) stimulates angiogenic signaling. Lipid rafts are cholesterol-dense regions of the plasma membrane that serve as an organizational platform for biomolecules. Although VEGFR2 has been shown to colocalize with lipid rafts to regulate its activation, the effect of lipid rafts on non-activated VEGFR2 has not been explored. Here, we characterized the involvement of lipid rafts in modulating the stability of non-activated VEGFR2 in endothelial cells using raft disrupting agents: methyl-β-cyclodextrin, sphingomyelinase and simvastatin. Disrupting lipid rafts selectively decreased the levels of non-activated VEGFR2 as a result of increased lysosomal degradation. The decreased expression of VEGFR2 translated to reduced VEGF-activation of the extracellular signal-regulated protein kinases (ERK). Overall, our results indicate that lipid rafts stabilize VEGFR2 and its associated signal transduction activities required for angiogenesis. Thus, modulation of lipid rafts may provide a means to regulate the sensitivity of endothelial cells to VEGF stimulation. Indeed, the ability of simvastatin to down regulate VEGFR2 and inhibit VEGF activity suggest a potential mechanism underlying the observation that this drug improves outcomes in the treatment of certain cancers.
Financing the Water and Sanitation Sectors: A Hybrid Literature Review
Authors: Machete Inês , Marques Rui
Published: January 2021

To ensure the sustainable development of the water and wastewater (WSS) sectors, new and more investments are needed. However, private financing in these sectors has not been successfully leveraged. This study conducted a systematic literature review of peer-reviewed papers in this field, to provide an overview of how researchers have been studying the financing endeavors of the WSS sectors. A three-part framework was carried out: retrieval of papers from search engines (Scopus and ASCE Library), focused on the title, abstract, and keywords; process of elimination of papers; and analysis of the selected papers based on the hybrid method combining a systematic quantitative review, a semantic network analysis, and a narrative analysis. This research resulted in the identification of four most studied areas: sustainable development; water management and public finance; project financing; and public policy. Future research should focus on existing financing instruments and how these can specifically be applied in the WSS sectors successfully. This paper makes several contributions to the literature because it addresses and emphasizes the most important financing issues in WSS, reviews the insights found, and discusses the future research needs. In addition, it demonstrates the benefits of using a hybrid methodology that could be adopted in other literature review studies.
Nanocomposites for Enhanced Osseointegration of Dental and Orthopedic Implants Revisited: Surface Functionalization by Carbon Nanomaterial Coatings
Authors: Kang Moon Sung , Lee Jong Ho , Hong Suck Won , Lee Jong Hun , Han Dong-Wook
Published: January 2021

Over the past few decades, carbon nanomaterials, including carbon nanofibers, nanocrystalline diamonds, fullerenes, carbon nanotubes, carbon nanodots, and graphene and its derivatives, have gained the attention of bioengineers and medical researchers as they possess extraordinary physicochemical, mechanical, thermal, and electrical properties. Recently, surface functionalization with carbon nanomaterials in dental and orthopedic implants has emerged as a novel strategy for reinforcement and as a bioactive cue due to their potential for osseointegration. Numerous developments in fabrication and biological studies of carbon nanostructures have provided various novel opportunities to expand their application to hard tissue regeneration and restoration. In this minireview, the recent research trends in surface functionalization of orthopedic and dental implants with coating carbon nanomaterials are summarized. In addition, some seminal methodologies for physicomechanical and electrochemical coatings are discussed. In conclusion, it is shown that further development of surface functionalization with carbon nanomaterials may provide innovative results with clinical potential for improved osseointegration after implantation.
Single Wall Carbon Nanotubes/Polypyrrole Composite Thin Film Electrodes: Investigation of Interfacial Ion Exchange Behavior
Authors: Escobar-Teran Freddy , Perrot Hubert , Sel Ozlem
Published: January 2021

Single-wall carbon nanotubes/polypyrrole (SWCNT/PPy) composite thin-film electrodes were prepared by electrodeposition of the pyrrole monomer on a porous network made of SWCNT bundles. Electrode/electrolyte interface, which is intimately related to the pseudocapacitive charge storage behavior, is investigated by using coupled electrogravimetric methods (electrochemical quartz crystal microbalance (EQCM) and its coupling with electrochemical impedance spectroscopy, Ac-electrogravimetry), in a 0.5 M NaCl electrolyte (pH = 7). Our results show that the range of usable potential is greater for composite SWCNT/PPy films than for SWCNT films, which should allow a higher storage capacity to be obtained. This effect is also confirmed by mass variation measurements via EQCM. The mass change (corresponding to the amount of (co)electroadsorbed species) obtained with composite SWCNT/PPy films is four times greater than that observed for pristine SWCNT films if the same potential range is examined. The permselectivity is also greatly improved in the case of composite SWCNT/PPy films compared to SWCNT films; the former shows mainly cation exchange preference. The quantities of anions estimated by Ac-electrogravimetric measurements are much lower in the case of composites. This corroborates the better permselectivity of these composite SWCNT/PPy films even with a moderate amount of PPy.
Facial Imitation Improves Emotion Recognition in Adults with Different Levels of Sub-Clinical Autistic Traits
Authors: Kowallik Andrea , Pohl Maike , Schweinberger Stefan
Published: January 2021

We used computer-based automatic expression analysis to investigate the impact of imitation on facial emotion recognition with a baseline-intervention-retest design. The participants: 55 young adults with varying degrees of autistic traits, completed an emotion recognition task with images of faces displaying one of six basic emotional expressions. This task was then repeated with instructions to imitate the expressions. During the experiment, a camera captured the participants’ faces for an automatic evaluation of their imitation performance. The instruction to imitate enhanced imitation performance as well as emotion recognition. Of relevance, emotion recognition improvements in the imitation block were larger in people with higher levels of autistic traits, whereas imitation enhancements were independent of autistic traits. The finding that an imitation instruction improves emotion recognition, and that imitation is a positive within-participant predictor of recognition accuracy in the imitation block supports the idea of a link between motor expression and perception in the processing of emotions, which might be mediated by the mirror neuron system. However, because there was no evidence that people with higher autistic traits differ in their imitative behavior per se, their disproportional emotion recognition benefits could have arisen from indirect effects of imitation instructions
The Smk1 MAPK and Its Activator, Ssp2, Are Required for Late Prospore Membrane Development in Sporulating Saccharomyces cerevisiae
Authors: Durant Matthew , Roesner Joseph M. , Mucelli Xheni , Slubowski Christian J. , Klee Erin , Seitz Brian C. , Wallis Zoey , Huang Linda S.
Published: January 2021

During sporulation in the budding yeast Saccharomyces cerevisiae, proper development of the prospore membrane is necessary for the formation of viable spores. The prospore membrane will eventually become the plasma membrane of the newly formed haploid spore and also serves as the template for the deposition of the spore wall. The prospore membrane is generated de novo during meiosis II and the growing edge of the prospore membrane is associated with the Leading Edge Protein (LEP) complex. We find that the Smk1 MAP kinase, along with its activator Ssp2, transiently localizes with the LEP during late meiosis II. SSP2 is required for the leading edge localization of Smk1; this localization is independent of the activation state of Smk1. Like other LEP components, the localization of Smk1 at the leading edge also depends on Ady3. Although prospore membrane development begins normally in smk1 and ssp2 mutants, late prospore membrane formation is disrupted, with the formation of ectopic membrane compartments. Thus, MAP kinase signaling plays an important role in the formation of the prospore membrane.
Land Cover Change in the Blue Nile River Headwaters: Farmers’ Perceptions, Pressures, and Satellite-Based Mapping
Authors: Ewunetu Alelgn , Simane Belay , Teferi Ermias , Zaitchik Benjamin F.
Published: January 2021

The headwaters of the Blue Nile River in Ethiopia contain fragile mountain ecosystems and are highly susceptible to land degradation that impacts water quality and flow dynamics in a major transboundary river system. This study evaluates the status of land use/cover (LULC) change and key drivers of change over the past 31 years through a combination of satellite remote sensing and surveying of the local understanding of LULC patterns and drivers. Seven major LULC types (forest land, plantation forest, grazing land, agriculture land, bush and shrub land, bare land, and water bodies) from Landsat images of 1986, 1994, 2007, and 2017 were mapped. Agriculture and plantation forest land use/cover types increased by 21.4% and 368.8%, respectively, while other land use/cover types showed a decreasing trend: water body by 50.0%, bare land by 7.9%, grassland by 41.7%, forest by 28.9%, and bush and shrubland by 38.4%. Overall, 34.6% of the landscape experienced at least one LULC transition over the past 31 years, with 15.3% representing the net change and 19.3% representing the swap change. The percentage change in plantation forest land increased with an increasing altitude and slope gradient during the study period. The mapped LULC changes are consistent with the pressures reported by local residents. They are also consistent with root causes that include population growth, land tenure and common property rights, persistent poverty, weak enforcement of rules and low levels of extension services, a lack of public awareness, and poor infrastructure. Hence, the drivers for LULC should be controlled, and sustainable resources use is required; otherwise, these resources will soon be lost and will no longer be able to play their role in socioeconomic development and environmental sustainability.
Influence of Lateral Movement on Level Behavior of Adhesion Force Measured Repeatedly by an Atomic Force Microscope (AFM) Colloid Probe in Dry Conditions
Authors: Li Ping , Lai Tianmao
Published: January 2021

An atomic force microscope (AFM) was operated to repeatedly measure the adhesion forces between a polystyrene colloid probe and a gold film, with and without lateral movement in dry conditions. Experimental results show that the adhesion force shows a level behavior without lateral movement and with a small scan distance: the data points are grouped into several levels, and the adhesion force jumps between different levels frequently. This was attributed to the fact that when the cantilever pulls off the sample, the contact area of the sample is not exactly the same between successive contacts and jumps randomly from one to another. Both lateral velocity and material wear have little influence on level behavior. However, with a medium scan distance, level behavior is observed only for some measurements, and adhesion forces are randomly distributed for the other measurements. With a large scan distance, adhesion forces are randomly distributed for all measurements. This was attributed to the fact that the cantilever pulls off the sample in many different contact areas on the scanning path for large distances. These results may help understand the influence of lateral movement and imply the contribution of asperities to adhesion force.
Impact of Warm Vertical Compaction on the Sealing Ability of Calcium Silicate-Based Sealers: A Confocal Microscopic Evaluation
Authors: Eid Diana , Medioni Etienne , De-Deus Gustavo , Khalil Issam , Naaman Alfred , Zogheib Carla
Published: January 2021

The aim of this in vitro study was to evaluate the dentinal tubule penetration of two calcium silicate-based sealers used in warm vertical compaction (WVC) obturation technique in comparison with the single cone (SC) technique by confocal laser scanning microscopy (CLSM). The null hypothesis was that both obturation techniques produced similar sealer penetration depths at 1 and 5 mm from the apex. Forty-four mandibular single-rooted premolars were randomly divided into four equally experimental groups (n = 10) and two control groups (n = 2) according to the type of sealer (Bio-C Angelus, Londrína, PR, Brazil or HiFlow Brasseler, Savannah, GA, USA) with either SC or WVC. The sealers were mixed with a fluorescent dye Rhodamine B (0.1%) to enable the assessment under the CLSM. All the specimens were sectioned horizontally at 1 and 5 mm from the apex. The maximum penetration depth was calculated using the ImageJ Software (ImageJ, NIH). Data were analyzed by Mann–Whitney U and Kruskal–Wallis tests (p < 0.05). A significant difference was shown between the four groups at 1 mm (p = 0.0116), whereas similar results were observed at 5 mm (p = 0.20). WVC allowed better diffusion for both sealers at 1 mm (p = 0.01) and 5 mm (p = 0.034). The maximum penetration of the Bio-C and HiFlow sealers was more important at 5 mm with the two obturation techniques. Within the limitations of this study, WVC enhanced the penetration of calcium silicate-based sealers into the dentinal tubules in comparison with the SC technique at both levels.
Surface Texturing of Si with Periodically Arrayed Oblique Nanopillars to Achieve Antireflection
Authors: Kim Jun-Hyun , You Sanghyun , Kim Chang-Koo
Published: January 2021

Si surfaces were texturized with periodically arrayed oblique nanopillars using slanted plasma etching, and their optical reflectance was measured. The weighted mean reflectance (Rw) of the nanopillar-arrayed Si substrate decreased monotonically with increasing angles of the nanopillars. This may have resulted from the increase in the aspect ratio of the trenches between the nanopillars at oblique angles due to the shadowing effect. When the aspect ratios of the trenches between the nanopillars at 0° (vertical) and 40° (oblique) were equal, the Rw of the Si substrates arrayed with nanopillars at 40° was lower than that at 0°. This study suggests that surface texturing of Si with oblique nanopillars reduces light reflection compared to using a conventional array of vertical nanopillars.
Effect of Flue Gas Desulfurization Gypsum on the Properties of Calcium Sulfoaluminate Cement Blended with Ground Granulated Blast Furnace Slag
Authors: Gao Danying , Zhang Zhenqing , Meng Yang , Tang Jiyu , Yang Lin
Published: January 2021

This work aims to investigate the effect of additional flue gas desulfurization gypsum (FGDG) on the properties of calcium sulfoaluminate cement (CSAC) blended with ground granulated blast furnace slag (GGBFS). The hydration rate, setting time, mechanical strength, pore structure and hydration products of the CSAC-GGBFS mixture containing FGDG were investigated systematically. The results show that the addition of FGDG promotes the hydration of the CSAC-GGBFS mixture and improves its mechanical strength; however, the FGDG content should not exceed 6%.
Shaping Microstructure and Mechanical Properties of High-Carbon Bainitic Steel in Hot-Rolling and Long-Term Low-Temperature Annealing
Authors: Dembiczak Tomasz , Knapiński Marcin
Published: January 2021

Based on the research results, coefficients in constitutive equations, describing the kinetics of dynamic, meta-dynamic, and static recrystallization in high-carbon bainitic steel during hot deformation were determined. The developed mathematical model takes into account the dependence of the changing kinetics in the structural size of the preliminary austenite grains, the value of strain, strain rate, temperature, and time. Physical simulations were carried out on rectangular specimens. Compression tests with a flat state of deformation were carried out using a Gleeble 3800. Based on dilatometric studies, coefficients were determined in constitutive equations, describing the grain growth of the austenite of high-carbon bainite steel under isothermal annealing conditions. The aim of the research was to verify the developed mathematical models in semi-industrial conditions during the hot-rolling process of high-carbon bainite steel. Analysis of the semi-industrial studies of the hot-rolling and long-term annealing process confirmed the correctness of the predicted mathematical models describing the microstructure evolution.
Evaluation of Antimicrobial Efficacy and Permeability of Various Sealing Materials at the Implant–Abutment Interface—A Pilot In Vitro Study
Authors: Smojver Igor , Vuletić Marko , Gerbl Dražena , Budimir Ana , Sušić Mato , Gabrić Dragana
Published: January 2021

The microenvironment of the oral cavity is altered when an implant, a biocompatible foreign body, is inserted into the mouth. Bacteria settle in the tissues in and around the implant due to the passage of microorganisms through the microgap at the connection of the implant and prosthetic abutment. To prevent colonization of the implant by microorganisms, one idea is to use sealing and antimicrobial materials to decontaminate the implant–abutment interface and close the microgap. The purpose of this study is to evaluate the antimicrobial efficacy and permeability of different types of sealing materials at the implant–abutment interface, under static conditions. Three different sealing material (GapSeal gel, Oxysafe gel and Flow.sil) were used for sealing the implant–abutment interfaces in 60 titanium dental implants, which were first contaminated with a solution containing Staphylococcus aureus and Candida albicans for 14 days under an aerobic condition. Results showed that a complete seal against bacterial infection was not formed at the implant–abutment interface, while for fungal infections, only GapSeal material helped to prevent microleakage. Findings of this in vitro study reported that application of sealing material before abutment connection may reduce peri-implant bacterial and fungal population compared with the interface without sealing material.
Biological Oriented Immediate Loading: A New Mathematical Implant Vertical Insertion Protocol, Five-Year Follow-Up Study
Authors: Bambini Fabrizio , Orilisi Giulia , Quaranta Alessandro , Memè Lucia
Published: January 2021

One of the current major challenges in implant therapy is to minimize marginal bone loss around implants, since it can trigger bacterial colonization of the implant’s neck, leading to its failure. The present study aimed (1) to scientifically validate a new mathematical rule based on soft tissues thickness, for choosing the correct implant position with respect to the bone level, in order to provide a better tissue adaptation to the abutment/implant surface to avoid bacterial invasion, and (2) to apply this mathematical rule to the Biological Oriented Immediate Loading (B.O.I.L.) surgical protocol, avoiding peri-implant bone resorption. N. 127 implants were inserted following B.O.I.L. protocol: implants were placed according to the mathematical rule Y = X − 3, which correlates the position of the implant from the bone crest level (Y) with the thickness of the soft tissues (X). All the implants were inserted in fresh extraction sockets, and immediately loaded with temporary abutments and prostheses. Bone levels were evaluated through radiographic examination just after surgical procedure (T0), and after 10 days (10D), 6 months (6M), 1 year (1Y), and 5 years (5Y). After 5 years, the implant survival rate was 100%, with a medium marginal bone loss around implants of 0.0704 mm (SD = 0.169 mm). One-way ANOVA, followed by Tukey’s multiple comparison test was performed for statistical evaluations (p < 0.05). This protocol provided a safe and successful procedure, with a good soft tissue seal against bacterial challenge. The application of the mathematical rule allows the implant placement in a correct vertical position from the bone crest, avoiding bone resorption and bacterial infiltrations. Moreover, the use of Multi Unit Abutment (MUA) determined a stable biological seal, favouring the implant healing and preserving the adhesion of hemidesmosomes to the titanium of MUA.
Optical Properties of Site-Selectively Grown InAs/InP Quantum Dots with Predefined Positioning by Block Copolymer Lithography
Authors: Holewa Paweł , Jasiński Jakub , Shikin Artem , Lebedkina Elizaveta , Maryński Aleksander , Syperek Marcin , Semenova Elizaveta
Published: January 2021

The InAs/InP quantum dots (QDs) are investigated by time-integrated (PL) and time-resolved photoluminescence (TRPL) experiments. The QDs are fabricated site-selectively by droplet epitaxy technique using block copolymer lithography. The estimated QDs surface density is ∼1.5 × 1010 cm−2. The PL emission at T=300 K is centered at 1.5 μm. Below T=250 K, the PL spectrum shows a fine structure consisting of emission modes attributed to the multimodal QDs size distribution. Temperature-dependent PL reveals negligible carrier transfer among QDs, suggesting good carrier confinement confirmed by theoretical calculations and the TRPL experiment. The PL intensity quench and related energies imply the presence of carrier losses among InP barrier states before carrier capture by QD states. The TRPL experiment highlighted the role of the carrier reservoir in InP. The elongation of PL rise time with temperature imply inefficient carrier capture from the reservoir to QDs. The TRPL experiment at T=15 K reveals the existence of two PL decay components with strong dispersion across the emission spectrum. The decay times dispersion is attributed to different electron-hole confinement regimes for the studied QDs within their broad distribution affected by the size and chemical content inhomogeneities.
In-Situ Laser Polishing Additive Manufactured AlSi10Mg: Effect of Laser Polishing Strategy on Surface Morphology, Roughness and Microhardness
Authors: Zhou Jiantao , Han Xu , Li Hui , Liu Sheng , Shen Shengnan , Zhou Xin , Zhang Dongqi
Published: January 2021

Laser polishing is a widely used technology to improve the surface quality of the products. However, the investigation on the physical mechanism is still lacking. In this paper, the established numerical transient model reveals the rough surface evolution mechanism during laser polishing. Mass transfer driven by Marangoni force, surface tension and gravity appears in the laser-induced molten pool so that the polished surface topography tends to be smoother. The AlSi10Mg samples fabricated by laser-based powder bed fusion were polished at different laser hatching spaces, passes and directions to gain insight into the variation of the surface morphologies, roughness and microhardness in this paper. The experimental results show that after laser polishing, the surface roughness of Ra and Sa of the upper surface can be reduced from 12.5 μm to 3.7 μm and from to 29.3 μm to 8.4 μm, respectively, due to sufficient wetting in the molten pool. The microhardness of the upper surface can be elevated from 112.3 HV to 176.9 HV under the combined influence of the grain refinement, elements distribution change and surface defects elimination. Better surface quality can be gained by decreasing the hatching space, increasing polishing pass or choosing apposite laser direction.
Corrosion Behavior of Cold-Formed AA5754 Alloy Sheets
Authors: Dobkowska Anna , Sotniczuk Agata , Bazarnik Piotr , Mizera Jarosław , Garbacz Halina
Published: January 2021

In this work, the influence of bending an AA5457 alloy sheet and the resulting microstructural changes on its corrosion behavior was investigated. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to perform detailed microstructural analyses of the alloy in its original form and after bending. After immersion in naturally-aged NaCl under open-circuit conditions (0.5 M, adjusted to 3 by HCl), post-corrosion observations were made, and electrochemical polarization measurements were performed to investigate the corrosion mechanisms occurring on both surfaces. The results showed that the corrosion of AA5457 is a complex process that mainly involves trenching around coarse Si-rich particles, crystallographically-grown large pits, and the formation of multiple tiny pits around Si-rich nanoparticles. The experimental data showed that bending AA5457 changed the shape and distribution of Si-rich coarse particles, cumulated a higher dislocation density in the material, especially around Si-rich nanoparticles, and all of these factors caused that corrosion behavior of the AA5754 in the bending area was lowered.
Comparative Study of Gelatin Hydrogels Modified by Various Cross-Linking Agents
Authors: Skopinska-Wisniewska Joanna , Tuszynska Marta , Olewnik-Kruszkowska Ewa
Published: January 2021

Gelatin is a natural biopolymer derived from collagen. Due to its many advantages, such as swelling capacity, biodegradability, biocompatibility, and commercial availability, gelatin is widely used in the field of pharmacy, medicine, and the food industry. Gelatin solutions easily form hydrogels during cooling, however, the materials are mechanically poor. To improve their properties, they are often chemically crosslinked. The cross-linking agents are divided into two groups: Zero-length and non-zero-length cross-linkers. In this study, gelatin was cross-linked by three different cross-linking agents: EDC-NHS, as a typically used cross-linker, and also squaric acid (SQ) and dialdehyde starch (DAS), as representatives of a second group of cross-linkers. For all prepared gelatin hydrogels, mechanical strength tests, thermal analysis, infrared spectroscopy, swelling ability, and SEM images were performed. The results indicate that the dialdehyde starch is a better cross-linking agent for gelatin than EDC-NHS. Meanwhile, the use of squaric acid does not give beneficial changes to the properties of the hydrogel.
Acoustic Properties of Innovative Concretes: A Review
Authors: Fediuk Roman , Amran Mugahed , Vatin Nikolai , Vasilev Yuriy , Lesovik Valery , Ozbakkaloglu Togay
Published: January 2021

Concrete is the most common building material; therefore, when designing structures, it is obligatory to consider all structural parameters and design characteristics such as acoustic properties. In particular, this is to ensure comfortable living conditions for people in residential premises, including acoustic comfort. Different types of concrete behave differently as a sound conductor; especially dense mixtures are superior sound reflectors, and light ones are sound absorbers. It is found that the level of sound reflection in modified concrete is highly dependent on the type of aggregates, size and distribution of pores, and changes in concrete mix design constituents. The sound absorption of acoustic insulation concrete (AIC) can be improved by forming open pores in concrete matrices by either using a porous aggregate or foam agent. To this end, this article reviews the noise and sound transmission in buildings, types of acoustic insulating materials, and the AIC properties. This literature study also provides a critical review on the type of concretes, the acoustic insulation of buildings and their components, the assessment of sound insulation of structures, as well as synopsizes the research development trends to generate comprehensive insights into the potential applications of AIC as applicable material to mitigate noise pollution for increase productivity, health, and well-being.
Scalable Output Linear Actuators, a Novel Design Concept Using Shape Memory Alloy Wires Driven by Fluid Temperature
Authors: Osorio Salazar Andres , Sugahara Yusuke , Matsuura Daisuke , Takeda Yukio
Published: January 2021

In this paper, the concept of scalability for actuators is introduced and explored, which is the capability to freely change the output characteristics on demand: displacement and force for a linear actuator, angular position and torque for a rotational actuator. This change can either be used to obtain power improvement (with a constant scale factor), or to improve the usability of a robotic system according to variable conditions (with a variable scale factor). Some advantages of a scalable design include the ability to adapt to changing environments, variable resolution of step size, ability to produce designs that are adequate for restricted spaces or that require strict energy efficiency, and intrinsically safe systems. Current approaches for scalability in actuators have shortcomings: the method to achieve scalability is complex, so obtaining a variable scaling factor is challenging, or they cannot scale both output characteristics simultaneously. Shape Memory Alloy (SMA) wire-based actuators can overcome these limitations, because its two output characteristics, displacement and force, are physically independent from each other. In this paper we present a novel design concept for linear scalable actuators that overcome SMA design and scalability limitations by using a variable number of SMA wires mechanically in parallel, immersed in a liquid that transmits heat from a separate heat source (wet activation). In this concept, more wires increase the maximum attainable force, and longer wires increase the maximum displacement. Prototypes with different number of SMA wires were constructed and tested in isometric experiments to determine force vs. temperature behavior and time response. The heat-transmitting liquid was either static or flowing using pumps. Scalability was achieved with a simple method in all tested prototypes with a linear correlation of maximum force to number of SMA wires. Flowing heat transmission achieved higher actuation bandwidth.
Tuning the Magnetocaloric Properties of the La(Fe,Si)13 Compounds by Chemical Substitution and Light Element Insertion
Authors: Paul-Boncour Valérie , Bessais Lotfi
Published: January 2021

LaFe13−xSix compounds exhibit a giant magnetocaloric effect and they are considered as a good magnetocaloric working substance for an environmentally friendly cooling technique. Nevertheless as the Curie temperature TC is around 200 K, it is necessary to tune TC near room temperature for magnetic refrigeration. In this work we present a review of the various methods of synthesis and shaping of the LaFe13−xSix type compounds as well as the influence of chemical substitution, light element insertion or combination of both on TC, magnetic entropy and adiabatic temperature variation (ΔSM and ΔTad), and stability upon cycling. The advantages and drawbacks of each method of preparation and type of element substitution/insertion are discussed. The implementation of these NaZn13 type materials in active magnetic refrigerator is presented and their performances are compared to that of Gd in prototypes.
Mathematical Modeling as a Catalyst for Equitable Mathematics Instruction: Preparing Teachers and Young Learners with 21st Century Skills
Authors: Suh Jennifer , Matson Kathleen , Seshaiyer Padmanabhan , Jamieson Spencer , Tate Holly
Published: January 2021

This case study focuses on a team of teachers and students in a Lesson Study, focused on using mathematical modeling (MM) to make significant decisions to design and plan for a sustainable edible garden in their community. We examined (a) how teachers develop students’ capacity to engage in mathematical modeling, while attending to equitable teaching practices; and (b) how teachers’ view of teaching through mathematics modeling changed after unit implementation. We found that teachers were deliberate in employing specific structures, routines, and tools to attend to equitable participation, when eliciting student thinking in the modeling process. We found that teachers’ view of mathematics modeling changed as they recognized how MM allowed for (a) integration of important mathematics concepts while giving students ownership of the mathematics; (b) opportunity to assess both content and 21st century process skills; and (c) positive energy that came from both students and teachers when teaching through the use of mathematical modeling. A promising strategy for preparing our youth for rigorous mathematics and skills to solve ill-structured problems is by integrating mathematical modeling in early elementary grades to develop critical 21st century skills and a productive disposition towards problem posing and problem solving.
In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects
Authors: Dutot Mélody , Olivier Elodie , Fouyet Sophie , Magny Romain , Hammad Karim , Roulland Emmanuel , Rat Patrice , Fagon Roxane
Published: January 2021

Phlorotannins are polyphenols occurring exclusively in some species of brown algae, known for numerous biological activities, e.g., antioxidant, antiproliferative, antidiabetic, and antiallergic properties. Their effects on the response of human lung cells to benzo[a]pyrene (B[a]P) has not been characterized. Our objective was to in vitro evaluate the effects of a phlorotannin-rich extract obtained from the brown algae Ascophyllum nodosum and Fucus vesiculosus on B[a]P cytotoxic effects. The A549 cell line was incubated with B[a]P for 48 and 72 h in the presence or absence of the brown algae extract. Cytochrome P450 activity, activation of P2X7 receptor, F-actin disorganization, and loss of E-cadherin expression were assessed using microplate cytometry and fluorescence microscopy. Relative to control, incubation with the brown algae extract was associated with lower B[a]P-induced CYP1 activity, lower P2X7 receptor activation, and lower reactive oxygen species production. The brown algae extract inhibited the alterations of F-actin arrangement and the downregulation of E-cadherin expression. We identified a phlorotannins-rich extract that could be deeper investigated as a cancer chemopreventive agent to block B[a]P-mediated carcinogenesis.
New Haloterpenes from the Marine Red Alga Laurencia papillosa: Structure Elucidation and Biological Activity
Authors: Shaaban Mohamed , Abou-El-Wafa Ghada S. E. , Golz Christopher , Laatsch Hartmut
Published: January 2021

Analysis of the air-dried marine red alga Laurencia papillosa, collected near Ras-Bakr at the Suez gulf (Red Sea) in Egypt delivered five new halogenated terpene derivatives: aplysiolic acid (1), 7-acetyl-aplysiol (2), aplysiol-7-one (3), 11,14-dihydroaplysia-5,11,14,15-tetrol (5a), and a new maneonene derivative 6, named 5-epi-maneolactone. The chemical structures of these metabolites were characterized employing spectroscopic methods, and the relative and absolute configurations were determined by comparison of experimental and ab initio-calculated NMR, NOE, ECD, and ORD data, and by X-ray diffraction of 2 and 6. The antimicrobial activities of the crude extract and compounds 1–3, 5a and 6 were studied.
Hazelnut Allergy
Authors: Calamelli Elisabetta , Trozzo Alessia , Di Blasi Elisabetta , Serra Laura , Bottau Paolo
Published: January 2021

Background and Objectives: Hazelnuts are frequently involved in IgE-mediated reactions and represent the main culprit of nut allergy in Europe. The clinical presentation varies from mild symptoms limited to the oropharynx [oral allergy syndrome (OAS)], due to the cross-reaction with homologues in pollen allergens and more severe events caused by the primary sensitization to highly stable molecules contained in hazelnuts. The aim of this review is to summarize the most relevant concepts in the field of hazelnut allergy and to provide a practical approach useful in the clinical practice Materials and Methods: References were identified by PubMed searches dating from January 2000 up to November 2020 using the search terms: “component resolved diagnosis” and “Hazelnut allergy. Results: The storage proteins Cor a 9 and Cor a 14 resulted highly specific for primary hazelnut allergy and strongly associated with severe reactions, while the cross reactive Cor a 1, an homolog of the birch Bet v1, were related to OAS. Any cut-off has shown a specificity and sensitivity pattern as high as to replace the oral food challenge (OFC), which still remains the gold standard in the diagnosis of hazelnut allergy. To date there is still no definitive treatment. Hazelnut free-diet and treatment of symptoms with emergency management, including the prescription of auto-injective epinephrine, still represent the main approach. Oral allergen immunotherapy (AIT) appears a promising therapeutic strategy and the definition of individual clinical threshold would be useful for sensitized individuals, caregivers, and physicians to reduce social limitation, anxiety, and better manage food allergy. Conclusions: An accurate diagnostic work-up including clinical history, in vivo and in vitro test including component resolved diagnosis and OFC are essential to confirm the diagnosis, to assess the risk of a severe reaction, and to prescribe an adequate diet and treatment.
Modelling the Molecular Permeation through Mixed-Matrix Membranes Incorporating Tubular Fillers
Authors: Zamani Ali , Tezel F. Handan , Thibault Jules
Published: January 2021

Membrane-based processes are considered a promising separation method for many chemical and environmental applications such as pervaporation and gas separation. Numerous polymeric membranes have been used for these processes due to their good transport properties, ease of fabrication, and relatively low fabrication cost per unit membrane area. However, these types of membranes are suffering from the trade-off between permeability and selectivity. Mixed-matrix membranes, comprising a filler phase embedded into a polymer matrix, have emerged in an attempt to partly overcome some of the limitations of conventional polymer and inorganic membranes. Among them, membranes incorporating tubular fillers are new nanomaterials having the potential to transcend Robeson’s upper bound. Aligning nanotubes in the host polymer matrix in the permeation direction could lead to a significant improvement in membrane permeability. However, although much effort has been devoted to experimentally evaluating nanotube mixed-matrix membranes, their modelling is mostly based on early theories for mass transport in composite membranes. In this study, the effective permeability of mixed-matrix membranes with tubular fillers was estimated from the steady-state concentration profile within the membrane, calculated by solving the Fick diffusion equation numerically. Using this approach, the effects of various structural parameters, including the tubular filler volume fraction, orientation, length-to-diameter aspect ratio, and permeability ratio were assessed. Enhanced relative permeability was obtained with vertically aligned nanotubes. The relative permeability increased with the filler-polymer permeability ratio, filler volume fraction, and the length-to-diameter aspect ratio. For water-butanol separation, mixed-matrix membranes using polydimethylsiloxane with nanotubes did not lead to performance enhancement in terms of permeability and selectivity. The results were then compared with analytical prediction models such as the Maxwell, Hamilton-Crosser and Kang-Jones-Nair (KJN) models. Overall, this work presents a useful tool for understanding and designing mixed-matrix membranes with tubular fillers.
Comprehensive Comparative Analysis of Local False Discovery Rate Control Methods
Authors: Kim Shin June , Oh Youngjae , Jeong Jaesik
Published: January 2021

Due to the advance in technology, the type of data is getting more complicated and large-scale. To analyze such complex data, more advanced technique is required. In case of omics data from two different groups, it is interesting to find significant biomarkers between two groups while controlling error rate such as false discovery rate (FDR). Over the last few decades, a lot of methods that control local false discovery rate have been developed, ranging from one-dimensional to k-dimensional FDR procedure. For comparison study, we select three of them, which have unique and significant properties: Efron’s approach, Ploner’s approach, and Kim’s approach in chronological order. The first approach is one-dimensional approach while the other two are two-dimensional ones. Furthermore, we consider two more variants of Ploner’s approach. We compare the performance of those methods on both simulated and real data.
Attitude and Heading Estimation for Indoor Positioning Based on the Adaptive Cubature Kalman Filter
Authors: Geng Jijun , Xia Linyuan , Wu Dongjin
Published: January 2021

The demands for indoor positioning in location-based services (LBS) and applications grow rapidly. It is beneficial for indoor positioning to combine attitude and heading information. Accurate attitude and heading estimation based on magnetic, angular rate, and gravity (MARG) sensors of micro-electro-mechanical systems (MEMS) has received increasing attention due to its high availability and independence. This paper proposes a quaternion-based adaptive cubature Kalman filter (ACKF) algorithm to estimate the attitude and heading based on smart phone-embedded MARG sensors. In this algorithm, the fading memory weighted method and the limited memory weighted method are used to adaptively correct the statistical characteristics of the nonlinear system and reduce the estimation bias of the filter. The latest step data is used as the memory window data of the limited memory weighted method. Moreover, for restraining the divergence, the filter innovation sequence is used to rectify the noise covariance measurements and system. Besides, an adaptive factor based on prediction residual construction is used to overcome the filter model error and the influence of abnormal disturbance. In the static test, compared with the Sage-Husa cubature Kalman filter (SHCKF), cubature Kalman filter (CKF), and extended Kalman filter (EKF), the mean absolute errors (MAE) of the heading pitch and roll calculated by the proposed algorithm decreased by 4–18%, 14–29%, and 61–77% respectively. In the dynamic test, compared with the above three filters, the MAE of the heading reduced by 1–8%, 2–18%, and 2–21%, and the mean of location errors decreased by 9–22%, 19–31%, and 32–54% respectively by using the proposed algorithm for three participants. Generally, the proposed algorithm can effectively improve the accuracy of heading. Moreover, it can also improve the accuracy of attitude under quasistatic conditions.