bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of SARS-CoV-2 Antibody Response Between Paired Fingerprick (HemaPEN®) and Venepuncture Collected Samples in Children and Adults
Authors: Mazarakis Nadia , Toh Zheng Quan , Nguyen Jill , Higgins Rachel A. , Rudge James , Whittle Belinda , Woudberg Nicholas J. , Devine Justin , Gooley Andrew , Lapierre Florian , Crawford Nigel W. , Tosif Shidan , Licciardi Paul V.
Published: February 2025

Serological surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is important to monitor population COVID-19 immunity. Dried blood spots (DBS) are a valuable method for serosurveys, particularly in remote settings and in children. We compared the measurement of SARS-CoV-2 spike-specific IgG in paired blood samples collected using standard venepuncture (serum) and the hemaPEN® microsampling DBS device from children and adults. A total of 83 participants (10 months to 65 years of age), comprising COVID-positive and -negative participants, were recruited. Paired serum and DBS samples were assayed for SARS-CoV-2 receptor-binding domain (RBD) and Spike (S1) antibodies using an established in-house ELISA. RBD and S1 IgG concentrations of paired hemaPEN DBS eluates and serum samples were compared using a non-parametric Wilcoxon matched-pairs signed ranked test. A Pearson’s correlation was used for RBD and S1 IgG concentrations and the level of agreement between the hemaPEN DBS eluates and serum samples was assessed by Bland–Altman analysis. A total of N = 41 adults (36 COVID-positive and 5 COVID-negative), and N = 42 children (37 COVID-positive, and 5 COVID-negative) have paired serum and DBS assayed. We found moderate to strong correlations between paired hemaPEN DBS eluates and serum SARS-CoV-2 IgG antibodies for RBD (r = 0.9472, p < 0.0001) and S1 proteins (r = 0.6892, p < 0.0001). Similar results were observed in both adult and paediatric populations. No significant differences in S1-specific IgG levels were observed in hemaPEN DBS samples stored for up to 35 weeks at room temperature. Eluted hemaPEN samples showed high specificity and sensitivity (100% and 89.89%, respectively) compared with serum. The use of the microsampling hemaPEN device for DBS sample collection is a feasible approach for assessing SARS-CoV-2 antibodies for serosurveillance studies, particularly in remote settings and in children.
Effects of High Temperature and Water Re-Curing on the Flexural Behavior and Mechanical Properties of Steel–Basalt Hybrid Fiber-Reinforced Concrete
Authors: Çelik Zinnur , Urtekin Yunus
Published: February 2025

Fiber-reinforced concrete (FRC) has become increasingly important in recent decades due to its superior mechanical properties, especially flexural strength and toughness, compared to normal concrete. FRC has also received significant attention because of its superior fire resistance performance compared to non-fiber concrete. In recent years, studies on the mechanical performance, fire design, and post-fire repair of thermally damaged fibrous and non-fibrous concrete have gained importance. In particular, there are very few studies in the literature on the mechanical performance and flexural behavior of steel and basalt hybrid fiber concretes after high temperature and water re-curing. This study aims to determine the mechanical properties and toughness of concrete containing steel fiber (SF) and basalt fiber (BF) after ambient and high temperature (400 °C, 600 °C, and 800 °C). Additionally, this study aimed to examine the changes in fire-damaged FRCs as a result of water re-curing. In this context, high temperature and water re-curing were carried out on non-fibrous concrete (control) and four different fiber compositions: in the first mixture, only steel fibers were used, and in the other two mixtures, basalt fibers were substituted at 25% and 50% rates instead of steel fibers. Furthermore, in the fifth mixture, basalt fibers were replaced by polypropylene fibers (PPFs) to make a comparison with the steel and basalt hybrid fiber-reinforced mixture. This study examined the effects of different fiber compositions on the ultrasonic pulse velocity (UPV) and compressive and flexural strength of the specimens at ambient temperature and after exposure to elevated temperatures and water re-curing. Additionally, the load–deflection curves and toughness of the mixtures were determined. The study results showed that different fiber compositions varied in their healing effect at different stages. The hybrid use of SF and BF can improve the flexural strength before elevated temperature and particularly after 600 °C. However, it caused a decrease in the recovery rates, especially after re-curing with water in terms of toughness. Water re-curing provided remarkable improvement in terms of mechanical and toughness properties. This improvement was more evident in steel–polypropylene fiber-reinforced concretes.
Systematic and Quantitative Assessment of Reduced Model Resolution on the Transient Structural Response Under Wind Load
Authors: Kodakkal Anoop , Péntek Máté , Bletzinger Kai-Uwe , Wüchner Roland , Weber Felix
Published: February 2025

The wind-induced response of structures is typically studied in wind tunnels either on scaled models or using numerical approaches under similar transient load conditions. In early design phases—where the potential for impactful change is most significant—information is often limited. As a result, studies are frequently conducted on simplified or reduced-resolution structural models. Typical applications for dimensionally reduced engineering models include early design phases, deciding on the need for high-fidelity analyses, and verifying wind tunnel models, which are often constructed using beams with lumped masses. In this contribution, the validity of these approaches is tested. Various limitations intrinsically arising from such modeling assumptions, showcased on a generic high-rise under dynamic wind load conditions, are highlighted. The systematic parametric analysis focuses on the variations in transient structural responses, particularly displacement and accelerations at the top of a building. Various wind loading cases are studied, with the reduction of the resolution taking place either in the original or in modal space. Results indicate that a considerable reduction is possible, but characteristic design values tend to deteriorate in cases of a high reduction, particularly when higher mode contributions are truncated. It is observed that the top-floor acceleration and displacement can be captured with considerable accuracy with three lumped masses for tall buildings. It is critical to study the impact of simplifying models starting at the highest level of detail possible. Here, a three-DoF model was able to capture the displacement up to a deviation of 11% and accelerations up to 20%. These approximate models are useful for initial design stages, optimization, uncertainty quantification, etc., where fast, cheap, and moderately accurate model evaluations are necessary.
Crack Propagation in Axial-Flow Fan Blades Under Complex Loading Conditions: A FRANC3D and ABAQUS Co-Simulation Approach
Authors: Ben Hassen Mariem , Ben-Elechi Slim , Mrad Hatem
Published: February 2025

Since fan blades are exposed to fatigue, and in some cases harsh loading conditions, they may exhibit fracture failures due to crack propagation, resulting in significant losses. Previous studies of crack propagation in blades are mainly confined to either simplified blade geometry or loads, resulting in a significant discrepancy between the simulated crack propagation and the real blade propagation behavior, while it is lacking for challenging shapes and loads. A co-simulation approach of FRANC3D and ABAQUS was developed to study the crack propagation of an axial-flow fan blade subjected to centrifugal, aerodynamic, and combined loads. The projected approach is validated with results obtained from analytical calculations and experiments. Meanwhile, making use of benchmarks, the Stress Intensity Factor (SIF) and the prediction of mixed-mode crack growth path are validated. Considering various loads, the crack propagation path response for the fan blade is computed for different growth steps. The results pinpoint that the crack propagation length of the crack tip center is maximum under centrifugal loading. However, the aerodynamic load led to a maximum propagation length of the crack tip endpoints. In addition, the combined force of centrifugal and aerodynamic loads limits the crack from growing.
High-Quality Classroom Dialogue Automatic Analysis System
Authors: Jia Linzhao , Sun Han , Jiang Jialong , Yang Xiaozhe
Published: February 2025

Classroom dialogue analysis is crucial as it significantly impacts both knowledge transmission and teacher–student interactions. Since the inception of classroom analysis research, traditional methods such as manual transcription and coding have served as foundational tools for understanding these interactions. While precise and insightful, these methods are inherently time-consuming, labor-intensive, and susceptible to human bias. Moreover, they struggle to handle the scale and complexity of modern classroom data effectively. In contrast, many contemporary deep learning approaches focus primarily on dialogue classification, but often lack the capability to provide deeper interpretative insights. To address these challenges, this study introduces an automated dialogue analysis system that combines scalability, efficiency, and objectivity in evaluating teaching quality. We first collected a large dataset of classroom recordings from primary and secondary schools in China and manually annotated the dialogues using multiple encoding frameworks. Based on these data, we developed an automated analysis system featuring a novel dialogue classification algorithm that incorporates speaker role information for more accurate insights. Additionally, we implemented innovative visualization techniques to automatically generate comprehensive classroom analysis reports, effectively bridging the gap between traditional manual methods and modern automated approaches. Experimental results demonstrated the system’s high accuracy in distinguishing various types of classroom dialogue. Large-scale analysis revealed key patterns in classroom dynamics, showcasing the strong potential of our system to enhance teaching evaluation and provide valuable insights for improving education practices.
Relationship Between Morning Blood Pressure Surges and Peripheral Inflammatory Biomarkers in Parkinson’s Disease
Authors: Sari Ummu S. , Yildirim Seda E. , Buyukserbetci Gulseren , Yildirim Tarik , Sackes Mesut , Esmeli Figen
Published: February 2025

Background: Parkinson’s disease (PD) is the second-most prevalent neurodegenerative disorder, often resulting in blood pressure abnormalities due to autonomic dysfunction. The early morning rise in blood pressure, referred to as the morning surge, has been associated with various cardiovascular diseases when exaggerated. This study aims to investigate the relationship between morning blood pressure surge (MBPS) and inflammatory markers in patients with PD. Methods: In this retrospective study, we employed 24 h ambulatory blood pressure monitoring alongside the fibrinogen-to-albumin ratio and high-sensitivity C-reactive protein (hs-CRP) as inflammatory markers. The study included fifty idiopathic PD patients and fifty age- and sex-matched control subjects. MBPS was defined as the difference between morning blood pressure (measured two hours after awakening) and the lowest recorded nighttime blood pressure. Body mass index (BMI) was considered as an independent variable. Results: Our study found that morning blood pressure surge (MBPS) levels were significantly higher in Parkinson’s disease (PD) patients compared to the control group, suggesting possible autonomic involvement. Conclusions: MBPS may indicate autonomic involvement, potentially contributing to cardiovascular and cerebral morbidity and mortality in PD patients. Longitudinal studies with larger sample sizes are warranted to further elucidate this relationship.
Preserflo-MicroShunt: Postoperative Effects on Endothelial Cell Density and Corneal Thickness
Authors: Dierse Sebastian , Esser Eliane Luisa , Merté Ralph-Laurent , Al-Nawaiseh Sami , Leclaire Martin Dominik , Eter Nicole , Brücher Viktoria Constanze
Published: February 2025

Background: The aim of this study was to evaluate the effects of Preserflo implantation on endothelial cell density (ECD), corneal thickness, intraocular pressure (IOP), and the use of antiglaucoma medications over a 12-month follow-up period. Methods: A total of 53 eyes from 53 patients undergoing Preserflo implantation were included in this prospective study. ECD, corneal thickness, IOP, and the number of antiglaucoma medications were measured at baseline, 3 months, and 12 months postoperatively. Statistical analysis was performed using paired t-tests and Wilcoxon signed-rank tests for non-normally distributed data. Results: There was no significant reduction in ECD at 3 months (p = 0.695) or 12 months (p = 0.229) compared to baseline. However, a significant reduction in corneal thickness was observed at 3 months (p = 0.008), with no significant change at 12 months (p = 0.118). A significant reduction in IOP was noted at both 3 months (from a preoperative median of 23.5 mmHg to 11.5 mmHg, p < 0.001) and 12 months (from 23.5 mmHg to 13.0 mmHg, p < 0.001). Additionally, there was a significant decrease in the use of antiglaucoma medications, from a preoperative median of 3.0 medication classes to 0.0 classes at both 3 months (p < 0.001) and 12 months (p < 0.001). Conclusions: Preserflo implantation resulted in a significant reduction in IOP and the need for antiglaucoma medications, with no significant impact on endothelial cell density and corneal thickness after 12 months. These findings suggest that Preserflo implantation is an effective procedure for IOP control and medication reduction, with favorable outcome for corneal health after one year.
Obesity: Recent Advances and Future Perspectives
Authors: Janić Miodrag , Janež Andrej , El-Tanani Mohamed , Rizzo Manfredi
Published: February 2025

As reported in the World Obesity Atlas 2024 by the World Obesity Federation, the projections for 2035 suggest that more than 1 [...]
Changes in Serum Inflammatory Markers and in Clinical Periodontal Condition After Non-Surgical Periodontal Treatment in Hypertensive Patients
Authors: Arregoces Francina María Escobar , Roa Nelly S. , Velosa-Porras Juliana , Rodríguez Lina Velásquez , Merchan María José , Poveda Jean Carlos Villamil , Otero Liliana , Ruiz Álvaro J. , Uriza Catalina Latorre
Published: February 2025

Background: Chronic inflammatory disorders, such as periodontitis, may contribute to pro-hypertensive inflammation. Objectives: This study aimed to analyze changes in parameters for periodontitis, such as periodontal inflamed surface area (PISA) and serum inflammatory markers, following non-surgical periodontal treatment in hypertensive patients. Methods: A quasi-experimental pre-and-post study was conducted, involving 42 controlled hypertensive patients with periodontitis. The patients underwent periodontal assessment and tests, including complete blood count, glucose, triglycerides, HDL-C, LDL-C, and serum levels of inflammatory biomarkers. All patients received scaling and root planning treatment in a single session and were reevaluated one month after the treatment. Results: Post-treatment evaluations showed significant improvements in periodontal inflammation parameters, such as pocket depth, attachment level, bleeding on probing, and biofilm percentage, with statistically significant differences (p < 0.001). There were decreases in serum VEGF levels (p < 0.001) and reductions in PISA associated with declines in cytokine levels such as IL-10, IL-6, IL-12p70, IL-17A, and VEGF. PISA for IL-6 and IL-10 had a positive correlation before periodontal treatment and with IL-1β and IL-10 after treatment. Conclusions: Hypertensive patients with periodontitis who underwent non-surgical periodontal treatment showed improvements in their periodontal condition, a decrease in cytokine levels such as VEGF, and reductions in PISA associated with declines in cytokines such as IL-10, IL-6, IL-12p70, IL-17A, and VEGF. These findings confirm the role of inflammation in hypertensive patients with periodontitis.
MHO: A Modified Hippopotamus Optimization Algorithm for Global Optimization and Engineering Design Problems
Authors: Han Tao , Wang Haiyan , Li Tingting , Liu Quanzeng , Huang Yourui
Published: February 2025

The hippopotamus optimization algorithm (HO) is a novel metaheuristic algorithm that solves optimization problems by simulating the behavior of hippopotamuses. However, the traditional HO algorithm may encounter performance degradation and fall into local optima when dealing with complex global optimization and engineering design problems. In order to solve these problems, this paper proposes a modified hippopotamus optimization algorithm (MHO) to enhance the convergence speed and solution accuracy of the HO algorithm by introducing a sine chaotic map to initialize the population, changing the convergence factor in the growth mechanism, and incorporating the small-hole imaging reverse learning strategy. The MHO algorithm is tested on 23 benchmark functions and successfully solves three engineering design problems. According to the experimental data, the MHO algorithm obtains optimal performance on 13 of these functions and three design problems, exits the local optimum faster, and has better ordering and stability than the other nine metaheuristics. This study proposes the MHO algorithm, which offers fresh insights into practical engineering problems and parameter optimization.
Correction: Roy et al. Metformin and ICG-001 Act Synergistically to Abrogate Cancer Stem Cells-Mediated Chemoresistance in Colorectal Cancer by Promoting Apoptosis and Autophagy. Cancers 2022, 14, 1281
Authors: Roy Souvick , Zhao Yinghui , Yuan Yate-Ching , Goel Ajay
Published: February 2025

In the original publication [...]
Ferroptosis-Related Gene Signatures: Prognostic Role in HPV-Positive Oropharyngeal Squamous Cell Carcinoma
Authors: Lenoci Deborah , Serafini Mara Serena , Lucchetta Marta , Cavalieri Stefano , Brakenhoff Ruud H. , Hoebers Frank , Scheckenbach Kathrin , Poli Tito , Licitra Lisa , De Cecco Loris
Published: February 2025

Background: Despite advances in the management of head and neck squamous cell carcinoma (HNSCC), prognostic models and treatment strategies remain inadequate, particularly for HPV-positive oropharyngeal squamous cell carcinoma (OPSCC). The rising incidence of HPV-positive OPSCC highlights an urgent need for innovative therapeutic approaches. Ferroptosis, a regulated form of non-apoptotic cell death, has gained attention for its role in cancer progression, but its potential as a prognostic and therapeutic target in HPV-positive OPSCC remains largely unexplored. This study investigates the role of ferroptosis in HPV-positive OPSCC, aiming to identify prognostic markers and provide insights into potential therapeutic strategies that could improve patient outcomes. Methods: Thirteen ferroptosis gene expression signatures were retrieved from the literature, and their performance and association to the immune microenvironment were validated on a meta-analysis of 267 HPV-positive cases (Metanalysis-HPV267) and 286 samples from the BD2Decide project (BD2-HPV286). Results: Our analysis revealed that specific ferroptosis-related gene expression signatures, particularly FER3, FER4, FER6, and FER12, are significantly associated (p-value < 0.05) with high-risk patient groups and adverse tumor microenvironment features, including suppressed immune activity and enhanced stromal involvement. Elevated expression of CAV1, a ferroptosis suppressor, further delineates high-risk profiles. Conclusions: These findings highlight the prognostic significance of ferroptosis in stratifying patients and identifying those with poorer clinical outcomes. Targeting ferroptosis pathways represents a novel and promising approach to addressing the unmet need for effective prognostic and therapeutic strategies in HPV-positive OPSCC. Future research should focus on translating these findings into clinical applications to advance precision oncology and improve outcomes for this growing patient population.
Ketone Bodies Are Potential Prognostic Biomarkers in Relapsed/Refractory Diffuse Large B-Cell Lymphoma: Results from the R2-GDP-GOTEL Trial
Authors: Fernández-Castillejo Sara , Badia Joan , de la Cruz-Merino Luís , Martín Garcia-Sáncho Alejandro , Carnicero-González Fernando , Palazón-Carrión Natalia , Ríos-Herranz Eduardo , de la Cruz-Vicente Fátima , Rueda-Domínguez Antonio , Martínez-Banaclocha Natividad , Gómez-Codina José , Labrador Jorge , Martínez-Madueño Francisca , Amigó Núria , Salar-Silvestre Antonio , Rodríguez-Abreu Delvys , Gálvez-Carvajal Laura , Sánchez-Beato Margarita , Provencio-Pulla Mariano , Guirado-Risueño Maria , Nogales Esteban , Sánchez-Margalet Víctor , Jiménez-Cortegana Carlos , Rodríguez-García Guillermo , Cumeras Raquel , Gumà Josep
Published: February 2025

Background: Patients with relapsed or refractory (R/R) diffuse large B-cell lymphoma (DLBCL) who are ineligible for high-dose chemotherapy have limited treatment options and poor life expectancy. The purpose of this study is to identify a serum metabolomic profile that may be predictive of outcome in patients with R/R-DLBCL. Methods: This study included 69 R/R DLBCL patients from the R2-GDP-GOTEL trial (EudraCT 2014-001620-299). Serum samples were collected at baseline, and the mean length of follow-up was 41 months. Serum metabolites were analyzed by nuclear magnetic resonance (NMR). Metabolites were correlated with treatment response, progression-free survival (PFS), and overall survival (OS). Results: Serum levels of 3-hydroxybutyrate (3OHB) and acetone were significantly (p < 0.001) associated with PFS (3OHB: hazard ratio [HR] 7.7, 95% confidence interval [CI] 2.5–24.1; acetone: HR 9.32, 95% CI 2.75–31.6) and OS (3OHB: HR 9.32, 95% CI 2.75–31.6; acetone: HR 1.92, 95% CI 1.36–2.69). Serum values of 141 µM for 3OHB and 40 µM for acetone were the optimal cutoffs associated with the survival outcomes. Elevated 3OHB levels (>141 μM) were specific to the ABC subtype of DLBCL, while acetone levels were elevated in both types of DLCBL but more pronounced in ABC cases. In a multivariate survival analysis, including the International Prognostic Index (IPI) score and refractoriness status (R/R), 3OHB and acetone remained significant. To aid oncologists employing the R2-GDP regime, we constructed PFS and OS nomograms for R/R-DLBCL risk stratification, incorporating 3OHB levels or acetone levels, IPI score, and refractoriness status. The nomogram with 3OHB and refractoriness status showed a time-dependent AUC of 0.86 for 6-month PFS and 0.84 for 12-month OS. These nomograms provide a comprehensive tool for individualized risk assessment and treatment optimization. Conclusions: The ketone bodies 3OHB and acetone are potential prognostic biomarkers of poor outcome in R/R DLBCL patients treated with the R2-GDP regimen, independently of IPI score and chemorefractoriness status.
S-Adenosylmethionine: A Multifaceted Regulator in Cancer Pathogenesis and Therapy
Authors: Fernández-Ramos David , Lopitz-Otsoa Fernando , Lu Shelly C. , Mato José M.
Published: February 2025

S-adenosylmethionine (SAMe) is a key methyl donor that plays a critical role in a variety of cellular processes, such as DNA, RNA and protein methylation, essential for maintaining genomic stability, regulating gene expression and maintaining cellular homeostasis. The involvement of SAMe in cancer pathogenesis is multifaceted, as through its multiple cellular functions, it can influence tumor initiation, progression and therapeutic resistance. In addition, the connection of SAMe with polyamine synthesis and oxidative stress management further underscores its importance in cancer biology. Recent studies have highlighted the potential of SAMe as a biomarker for cancer diagnosis and prognosis. Furthermore, the therapeutic implications of SAMe are promising, with evidence suggesting that SAMe supplementation or modulation could improve the efficacy of existing cancer treatments by restoring proper methylation patterns and mitigating oxidative damage and protect against damage induced by chemotherapeutic drugs. Moreover, targeting methionine cycle enzymes to both regulate SAMe availability and SAMe-independent regulatory effects, particularly in methionine-dependent cancers such as colorectal and lung cancer, presents a promising therapeutic approach. Additionally, exploring epitranscriptomic regulations, such as m6A modifications, and their interaction with non-coding RNAs could enhance our understanding of tumor progression and resistance mechanisms. Precision medicine approaches integrating patient subtyping and combination therapies with chemotherapeutics, such as decitabine or doxorubicin, together with SAMe, can enhance chemosensitivity and modulate epigenomics, showing promising results that may improve treatment outcomes. This review comprehensively examines the various roles of SAMe in cancer pathogenesis, its potential as a diagnostic and prognostic marker, and its emerging therapeutic applications. While SAMe modulation holds significant promise, challenges such as bioavailability, patient stratification and context-dependent effects must be addressed before clinical implementation. In addition, better validation of the obtained results into specific cancer animal models would also help to bridge the gap between research and clinical practice.
Right-Sided Versus Left-Sided Colon Cancer—A 5-Year Single-Center Observational Study
Authors: Szostek Julia , Serafin Michał , Mąka Magdalena , Jabłońska Beata , Mrowiec Sławomir
Published: February 2025

Background: Global colorectal cancer (CRC) incidence is significant, constituting 15% of all cancer cases with 1.4 million new diagnoses annually. Recent research suggests categorizing CRC into three clinical groups: right colon cancer (RCC), left colon cancer (LCC), and rectal cancer, each with distinct embryological and molecular characteristics. Methods: A retrospective analysis of 189 patients (103 men, 86 women) undergoing surgery for RCC and LCC from January 2018 to December 2023 was performed. Results: LCC was a more common localization (98, 51.85%) than RCC (91, 48.15%). Patients with RCC were older than patients with LCC (70 (36–92, IQR 11) vs. 68 (38–84, IQR 12.5) years; p = 0.02). The duration of surgical procedure was comparable in both groups (225 (120–420, IQR 80) vs. 210 (105–505, IQR 85) minutes; p = 0.16). Complications occurred in 16 (17.58%) patients with RCC and in 15 (15.31%) patients with LCC (p = 0.72). One-year overall survival was 92.76% (SE 2.16%) (91.57% (SE 3.43%) in the RCC group and 93.99% (SE 2.61%) in the LCC group; p = 0.79). Conclusions: Colon cancer incidence is increasing globally due to economic and lifestyle factors. Our study reflects this trend, noting a rise in cases from 2018 to 2023. Despite several differences, overall survival rates do not significantly differ between RCC and LCC patients. Understanding clinical disparities is crucial for optimizing patient outcomes.
Adsorption Studies of Salmonella Enteritidis and Escherichia coli on Chitosan-Coated Magnetic Nanoparticles
Authors: Franco Anthony James , Alocilja Evangelyn
Published: February 2025

One of the challenges of microbiological testing is the complex and lengthy sample preparation, causing delays in getting the final result. Immunomagnetic separation is one of the sample preparation techniques recently used to overcome this complexity. However, it is expensive, fragile, and requires cold storage. This study aimed to use chitosan-coated magnetic nanoparticles (cMNP) to capture bacterial cells from a simulated matrix and understand the interaction between the bacteria and the cMNP using batch adsorption studies. To illustrate the concept, Salmonella Enteritidis and Escherichia coli were used. Results showed that the adsorption of Salmonella Enteritidis and E. coli fitted the pseudo-second-order kinetic model (R2 = 0.939 and 0.968, respectively) and the Freundlich isotherm model (R2 = 0.999 and 0.970, respectively). The increased ionic strength enhanced bacterial adsorption, and the highest capture efficiency was observed at pH 4 (32.8% and 98.1% for Salmonella Enteritidis and E. coli, respectively). These results show that chemisorption plays a significant role in bacterial adsorption to cMNP. Furthermore, increasing ionic strength and acidic pH (pH 4) significantly affects the adsorption of Salmonella Enteritidis and E. coli on cMNP, making them crucial for enhancing the performance of cMNP-based sample preparation methods.
Novel Continuous Laboratory Reactor with Selectively Tempered Capillary for Hyaluronic Acid Depolymerization: Design, Optimization, and Proof-of-Concept
Authors: Pokorný Marek , Brýdl Adam , Čechovič Juraj , Pecháček Jakub , Velebný Vladimír
Published: February 2025

Hyaluronic acid (HA), a natural polymer produced through biotechnological processes, is initially synthesized with a high molecular weight, which is subsequently reduced for specific applications. This work aims to develop an experimental laboratory device that enables the controlled depolymerization of HA to achieve a targeted molecular weight. This is accomplished by applying precisely regulated temperature and pressure conditions that facilitate the cleavage of HA polymer chains as they pass through a narrow capillary. The process also allows for a controlled exposure time to elevate the temperature and pressure, with the rapid transition between standard and elevated conditions ensuring temporal stability. A key innovation of this approach is the selective application of an elevated temperature to a designated capillary section, adjustable from 0.5 to 5 m in 0.5 m increments, allowing for a broad range of reaction times. Numerical simulations verified temperature distribution along the capillary during heating and cooling. The device’s effectiveness is demonstrated by cleaving HA chains (initial Mw = 2150 kDa) dissolved in an aqueous solution at a 0.1 mg/mL concentration. Results from a factorial experiment that evaluated the extremes of three main variables show cleavage across a wide molecular weight range, reaching values as low as 8 kDa, with a variance of less than 5%. This study presents a viable device for the selective depolymerization of HA via physical parameters alone, eliminating the need for additional substances such as acids, hydroxides, or enzymes.
Outcomes of Pediatric Patients with Excessive Postural Tachycardia After Attending an Intensive Interdisciplinary Pain Program: A Pilot Study
Authors: Harbeck-Weber Cynthia , Klaas Kelsey , Sim Leslie , Weiss Karen , Shappell April , Harrison Tracy
Published: February 2025

Background/Objectives: Adolescents with autonomic disorders who attend Intensive Interdisciplinary Pain Treatment (IIPT) programs report improvements in functioning. However, it is unclear whether they experience corresponding improvements in physiological measures. As such, the aim of this pilot study was to examine changes in physiological measures in youth attending an IIPT program who demonstrated excessive postural tachycardia on an active stand test. The secondary goal was to examine associations between physiological measurements and self-reported measures of chronic orthostatic intolerance (cOI) and functioning. Methods: At admission and discharge, eighteen adolescents and young adults (AYAs) attending IIPT (M age = 17.39 years; SD = 2.15 years) completed an active stand test, measures of breathing rate and muscle tension, as well as self-reported measures of cOI symptoms and functioning. Results: AYAs showed significant reduction in active stand test heart rate increase (p < 0.001; d = 1.07) and maximum heart rate (p = 0.002, d = 0.76) from admission to discharge. Improvements were also observed in resting respiration rate (p = 0.001, d = 89) and resting trapezoid tension (p = 0.03, d = 0.49). Although patients showed significant improvements on self-report measures of functioning (p < 0.001, d = 1.78), changes on subjective report of cOI symptoms did not reach significance. Exploratory analyses that only included patients with a POTS diagnosis were consistent with the overall results. Conclusions: Youth who demonstrated excessive postural tachycardia on active stand test at admission to an IIPT showed significant improvements from admission to discharge in their active stand maximum heart rate and heart rate increase, as well as respiration rate, muscle tension, and reports of their functioning. Future research is necessary to examine the mechanisms of change that contribute to symptom improvement.
Innovative Carwash Wastewater Treatment and Reuse Through Nature-Based Solutions
Authors: Torrens Antonina , Sepúlveda-Ruiz Paola , Aulinas Montserrat , Folch Montserrat
Published: February 2025

Vehicle washing facilities (VWFs) consume substantial amounts of potable water and produce wastewater containing pollutants such as hydrocarbons, detergents, and pathogens, presenting significant environmental and operational challenges. This study evaluates Nature-based Solutions (NbS) for wastewater treatment and recycling at a pilot facility in Girona, Spain, aiming to reduce potable water consumption and ensure safe reuse while minimizing environmental impact. Over a two-year period, three systems—a Vertical Flow Treatment Wetland (VFTW), Horizontal Flow Treatment Wetland (HFTW), and Infiltration-Percolation (IP) filter—were tested. Thirty-two parameters, including physicochemical (e.g., turbidity, nutrients, heavy metals, detergents) and microbiological indicators (e.g., E. coli, Legionella spp.), were monitored. VFTW and IP systems were the most effective, reducing turbidity below 5 NTU, COD to under 20 mg/L, and E. coli below 10 CFU/100 mL, meeting Spanish reuse standards. The HFTW effectively removed organic matter and nutrients but faced challenges such as clogging and reduced hydraulic performance, making it less suitable for carwash wastewater. Together, these systems enabled up to 60% water reuse, with final chlorination ensuring microbial safety, particularly against Legionella, while meeting Spanish reuse standards. This study highlights the potential of NbS as sustainable, low-energy solutions for wastewater recycling and pollution control in vehicle washing facilities.
Novel GPU-Based Method for the Generalized Maximum Flow Problem
Authors: Spridon Delia Elena , Deaconu Adrian Marius , Tayyebi Javad
Published: February 2025

This paper investigates the application of a minimum loss path finding algorithm to determine the maximum flow in generalized networks that are characterized by arc losses or gains. In these generalized network flow problems, each arc has not only a defined capacity but also a loss or gain factor, which must be taken into consideration when calculating the maximum achievable flow. This extension of the traditional maximum flow problem requires a more comprehensive approach, where the maximum amount of flow is determined by accounting for additional factors such as costs, varying arc capacities, and the specific loss or gain associated with each arc. This paper extends the classic Ford–Fulkerson algorithm, adapting it to iteratively identify source-to-sink (s − t) residual directed paths with minimum cumulative loss and generalized augmenting paths (GAPs), thus enabling the efficient computation of maximum flow in such complex networks. Moreover, to enhance the computational performance of the proposed algorithm, we conducted extensive studies on parallelization techniques using graphics processing units (GPUs). Significant improvements in the algorithm’s efficiency and scalability were achieved. The results demonstrate the potential of GPU-accelerated computations in handling real-world applications where generalized network flows with arc losses and gains are prevalent, such as in telecommunications, transportation, or logistics networks.
Association Between Biomass Fuel Use and Depression Symptoms in the Adult Population of Oaxaca, Mexico
Authors: Abeldaño Zuñiga Roberto Ariel , Coca Silvia Mercedes , Foláyan Moréniké Oluwátóyin , Fanta Garrido Javiera , de Lima Gabriela Narcizo
Published: February 2025

Background: The impact of biomass fuel exposure on mental health, along with the associated gender disparities, remains largely unexplored. This study aimed to examine the association between biomass fuel use and depressive symptoms in the population of Oaxaca, Mexico, while also identifying gender differences in this relationship. Methods: This study used data from the 2022 National Health and Nutrition Survey (ENSANUT). Depressive symptoms, the outcome variable, were assessed using the Center for Epidemiologic Studies Depression Scale (CESD). The primary predictor variable was biomass fuel use, with gender, age, and residency stratum included as covariates. First, a binary logistic regression model was developed to estimate the dichotomous variable “depression symptoms”. Subsequently, a second binary logistic regression model was constructed to evaluate potential interactions between the covariates and the predictor variable. Findings: The sample included 1.4 million adults from Oaxaca, with a prevalence of depressive symptoms of 15%. Biomass fuel was used by 15.4% of the population. The first logistic regression model showed that women (Odds Ratio (OR): 1.249; 95% CI: 1.235–1.263; p < 0.001), individuals aged 60 years and older compared to the younger population group (OR: 12.192; 95% CI: 12.064–12.321; p < 0.001), those residing in rural areas (OR: 1.245; 95% CI: 1.232–1.259; p < 0.001), and individuals using firewood or charcoal for cooking (OR: 1.674; 95% CI: 1.651–1.697; p < 0.001) had higher odds of depressive symptoms. In the second binary logistic regression model, all associations and OR coefficients retained their direction, although the coefficients underwent a slight adjustment following the introduction of the interaction term, indicating the presence of an interaction. Conclusions: The study findings suggest a gendered association between biomass fuel exposure and depressive symptoms in the adult population of Oaxaca, with older women and women dwelling in rural areas being the most vulnerable. Interventions aimed at reducing biomass air pollution exposure and strengthening mental health support for women are strongly recommended.
Analysis of the Impact of SMEs’ Production Output on Kazakhstan’s Economic Growth Using the ARDL Method
Authors: Syzdykova Aziza , Azretbergenova Gulmira
Published: February 2025

Small and medium-sized businesses (SMEs) are an essential subject of economic activity in any country because, without their participation, the development and formation of the very structure of the economy are almost impossible. The role of SMEs is significant since these businesses allow for an increase in the number of jobs, develop competition, and, as a result, improve the quality of goods, creating different price segments. More than 4 million people are employed in this sector in the Republic of Kazakhstan, and their share of GDP is 36.7%. The accelerated contribution of the SME sector to Kazakhstan’s GDP has led to the need to conduct a study in this area. This study analyzes the impact of SME production output on Kazakhstan’s economic growth by considering some macroeconomic variables using the ARDL model. The study’s findings confirm that SME output positively and significantly impacts economic growth. The government of Kazakhstan has been implementing a series of policies and incentive programs to increase the contribution of the SME sector to economic growth since the years of independence. However, SMEs are not able to reach their full potential due to various restrictions that limit their expansion. This study offers some suggestions for the development of the SME sector. In order to ensure SME concentration in the economy, investment in R&D should be a priority incentive. On the other hand, we should recognize the shadow economy problem in the country.
The Development of a Train-the-Trainer Approach in Reciprocal Reading Instruction in England’s Primary Schools
Authors: Cockerill Maria , O’Keeffe Joanne , Thurston Allen , Ozkaya Canan , Holland Joanne , Taylor Andy
Published: February 2025

There is good evidence that targeted reciprocal reading instruction for struggling readers can lead to attainment gains in reading for children aged 9–12 years in England using the FFT Reciprocal Reading instruction programme when training is delivered by the programme developer. However, for programme scale-up, a train-the-trainer delivery approach is required. This study reports the development of a train-the-trainer delivery approach during a Phase-1 pilot to enable the programme’s delivery at greater scale. During the pilot, five trainers were trained to cascade training to 16 English primary schools, which implemented the programme with 217 children. The train-the-trainer approach developed used a workshop and cascade model recommended by both health and education sectors. Trainer and school staff perception feedback was collected during the development process, and independently designed standardised reading tests were used to explore children’s progress during the pilot. Feedback suggests that the train-the-trainer approach developed is implementable in schools, including observable pupil progress in reading. This train-the-trainer delivery approach should now be tested using RCT methodology.
Leadership Practices That Enable and Constrain Retention in Early Childhood Education and Care Settings in Australia
Authors: Garrett Suzanne , Gibbs Leanne
Published: February 2025

Australia’s early childhood education and care (ECEC) sector is in crisis, with educator job tenure decreasing and workforce supply threatened. Despite worsening retention rates, many highly skilled educators remain and thrive within ECEC settings, ensuring strong relationships and positive outcomes for children. While research has uncovered the complex organisational factors contributing to workforce retention, the leadership’s influence on educator retention remains under-researched. This paper describes a small-scale Australian qualitative study of ECEC leaders and educators using a social constructivist lens. The present study explored how positional leaders in two high-quality ECEC sites enabled educator retention, considering the perspectives of leaders and educators. Data from semi-structured interviews were analysed using the theory of practice architectures to uncover the practices and practice architectures that enable and constrain retention. This research discovered six key practices of leaders. These six practices were flexible approaches to managing individuals, proactive approaches to creating and building professional relationships with educators, prioritising educators’ professional growth, positioning themselves to enable educators’ practice, creating cohesive teams, and valuing educators and their work. The practices of leaders and practice architectures illuminated in this study may be employed by organisations in the sector to support the retention of educators.
Induction Motor Geometric Parameter Optimization Using a Metaheuristic Optimization Method for High-Efficiency Motor Design
Authors: Apaydin Hasbi , Oyman Serteller Necibe Füsun , Oğuz Yüksel
Published: February 2025

In this study, the optimum design for an induction motor (IM) was realized by providing details of its geometric design. The IM optimization was carried out using the Artificial Ecosystem-based Optimization (AEO) algorithm, a metaheuristic method. The AEO algorithm was used for the first time in IM optimization, and the design parameters were optimized. Ten motor design parameters were used as design variables. IM efficiency was improved, as the objective function. The genetic algorithm (GA) optimization method was used for comparison with the results obtained with the AEO method. The optimized and unoptimized results of the IM design generated with codes created in the Matlab program were verified with the Ansys RMxprt EM Suite 19.2 program, and it could be seen that the results are in good agreement. As a result of these studies, it was observed that the use of AEO in determining the geometric parameters of the IM had better convergence accuracy and reached the optimum result in a shorter time compared to the GA optimization method. It was observed that IM efficiency increased from 90.34% to 91.575% on average with the AEO method.
Epigenomic Echoes—Decoding Genomic and Epigenetic Instability to Distinguish Lung Cancer Types and Predict Relapse
Authors: Baumann Alexandra A. , Buribayev Zholdas , Wolkenhauer Olaf , Salybekov Amankeldi A. , Wolfien Markus
Published: February 2025

Genomic and epigenomic instability are defining features of cancer, driving tumor progression, heterogeneity, and therapeutic resistance. Central to this process are epigenetic echoes, persistent and dynamic modifications in DNA methylation, histone modifications, non-coding RNA regulation, and chromatin remodeling that mirror underlying genomic chaos and actively influence cancer cell behavior. This review delves into the complex relationship between genomic instability and these epigenetic echoes, illustrating how they collectively shape the cancer genome, affect DNA repair mechanisms, and contribute to tumor evolution. However, the dynamic, context-dependent nature of epigenetic changes presents scientific and ethical challenges, particularly concerning privacy and clinical applicability. Focusing on lung cancer, we examine how specific epigenetic patterns function as biomarkers for distinguishing cancer subtypes and monitoring disease progression and relapse.
Mapping of Dominant Tree Species in Yunnan Province Based on Sentinel-2 Time-Series Data and Assessment of the Influence of Understory Background on Mapping Accuracy
Authors: Sun Yihao , Zhu Jingyuan , Yang Ben , Liu Haodong
Published: February 2025

Accurate information on the location of dominant tree species is essential for scientific forest management. However, factors like changes in forest phenology, stand conditions, and mixed understory backgrounds introduce uncertainties in remote sensing-based species mapping. To address these challenges, this study maps dominant tree species using time series Sentinel-2 data combined with environmental context data. To quantify the impact of understory background on mapping accuracy, this study applied a random forest inversion model to estimate the canopy cover across the study area. Binary contour plots and Pearson’s correlation coefficient were used to quantify the relationship between canopy cover and classification uncertainty at both the grid and pixels. A 10 m resolution map of dominant tree species in Yunnan Province, featuring eight species, was produced with an overall accuracy of 83.52% and a Kappa coefficient of 0.8115. The R2 value between the predicted and actual tree area proportions was greater than 0.93, with RMSEs consistently below 2.6. In addition, we observed strong negative correlations between different canopy cover classes. The correlations were −0.67 for low-cover areas, −0.40 for medium-cover areas, and −0.73 for high-cover areas. Our mapping framework enables the accurate identification of regional dominant species, and the established relationship between understory context and classification uncertainty provides valuable insights for analyzing potential mapping errors.
Feature Review Papers in Microbial Metabolism, Physiology and Genetics
Authors: Willaert Ronnie G.
Published: February 2025

This inaugural Special Issue of Fermentation (MDPI) features review articles spanning various aspects of microbial metabolism, physiology, and genetics [...]