bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploration of factors affecting hemodynamic stability following pheochromocytoma resection - cohort study
Authors: Liu Lidan , Shang Lihua , Zhuang Yimeng , Su Xiaojing , Li Xue , Sun Yumeng , Long Bo
Published: April 2024

Purpose

Surgery is the only way to cure pheochromocytoma; however, postoperative hemodynamic instability is one of the main causes of serious complications and even death. This study’s findings provide some guidance for improved clinical management.

Patients and methods

This study was to investigate the factors leading to postoperative hemodynamic instability in the postoperative pathology indicated pheochromocytoma from May 2016 to May 2022. They were divided into two groups according to whether vasoactive drugs were used for a median number of days or more postoperatively. The factors affecting the postoperative hemodynamics in the perioperative period (preoperative, intraoperative, and postoperative) were then evaluated.

Results

The median number of days requiring vasoactive drug support postoperatively was three in 234 patients, while 118 (50.4%) patients required vasoactive drug support for three days or more postoperatively. The results of the multivariate analysis indicated more preoperative colloid use (odds ratio [OR]=1.834, confidence interval [CI]:1.265–2.659, P=0.001), intraoperative use of vasoactive drug (OR=4.174, CI:1.882–9.258, P<0.001), and more postoperative crystalloid solution input per unit of body weight per day (ml/kg/d) (OR=1.087, CI:1.062–1.112, P<0.001) were risk factors for predicting postoperative hemodynamic instability. The optimal cutoff point of postoperative crystalloid use were 42.37 ml/kg/d.

Conclusion

Hemodynamic instability is a key issue for consideration in the perioperative period of pheochromocytoma. The amount of preoperative colloid use, the need for intraoperative vasoactive drugs, and postoperative crystalloid solution are risk factors for predicting postoperative hemodynamic instability (registration number: ChiCT2300071166).
Association between metabolic syndrome severity score and cardiovascular disease: results from a longitudinal cohort study on Chinese adults
Authors: Lin Jing-jing , Dai Pin-yuan , Zhang Jie , Guan Yun-qi , Gong Wei-wei , Yu Min , Fang Le , Hu Ru-ying , He Qing-fang , Li Na , Wang Li-xin , Liang Ming-bin , Zhong Jie-ming
Published: April 2024

Objective

This study aimed to quantify the severity of metabolic syndrome(MetS) and investigate its association with cardiovascular disease(CVD) risk on Chinese adults.

Methods

13,500 participants from the Zhejiang Adult Chronic Disease Study were followed up between 2010 and 2021. A continuous MetS severity score derived from the five components of MetS was used to quantify MetS severity, and the association between MetS severity and the risk of incident CVD was assessed using Cox proportional hazard and restricted cubic spline regression.

Results

Both the presence and severity of MetS were strongly associated with CVD risk. MetS was related to an increased risk of CVD (hazard ratio(HR):1.700, 95% confidence interval(CI): 1.380–2.094). Compared with the hazard ratio for CVD in the lowest quartile of the MetS severity score, that in the second, third, and highest quartiles were 1.812 (1.329–2.470), 1.746 (1.265–2.410), and 2.817 (2.015–3.938), respectively. A linear and positive dose-response relationship was observed between the MetS severity and CVD risk (P for non-linearity = 0.437). Similar results were found in various sensitivity analyses.

Conclusion

The MetS severity score was significantly associated with CVD risk. Assessing MetS severity and further ensuring intervention measures according to the different severities of MetS may be more useful in preventing CVD.
α-Klotho levels in girls with central precocious puberty: potential as a diagnostic and monitoring marker
Authors: Park Jun-Hong , Noh Eu-Seon , Hwang Il Tae
Published: April 2024

Background

Recent studies suggest a link between the Klotho protein, sex hormones, and insulin-like growth factor-1 (IGF-1), indicating that α-Klotho levels may rise during puberty, including in central precocious puberty (CPP) cases. This study aimed to explore α-Klotho levels in girls with CPP to assess its potential as a diagnostic and monitoring tool for this condition.

Methods

In total, 139 girls, comprising 82 patients diagnosed with CPP and 57 healthy prepubertal controls, were enrolled in this study. From March 2020 to May 2023, we assessed both α-Klotho levels and clinical parameters. α-Klotho concentrations were measured using an α-Klotho ELISA kit. For the girls with CPP, we additionally analyzed samples taken 6 months after GnRH agonist treatment.

Results

α-Klotho levels were higher in the CPP group compared with the control (CPP group: 2529 ± 999 ng/mL; control group: 1802 ± 675 pg/mL) (P < 0.001), and its level modest decreased after 6 months of GnRH agonist treatment (2147± 789 pg/mL) (P < 0.001). The association between α-Klotho and IGF-1 SDS, follicular stimulating hormone and baseline luteinizing hormone was assessed by partial correlation after adjusting for age, BMI SDS (r= 0.416, p= <0.001; r= 0.261, p= 0.005; r= 0.278, p= 0.002), respectively. Receiver operating characteristic curve analysis identified an α-Klotho cut-off differentiating CPP from controls, with a cut-off of 1914 pg/mL distinguishing girls with CPP from controls with a sensitivity of 69.5% and specificity of 70.2%; the area under the curve was 0.723.

Conclusion

The findings of our study are the first step towards deciphering the role of α-Klotho in puberty induction. With additional data and further research, α-Klotho could potentially be utilized as a significant diagnostic and monitoring tool for CPP.
Strategy for evaluating the status of relay protection equipment for the new generation of intelligent substations
Authors: Liu Dapeng , Wu Kuizhong , Guo Zhenhua , Cui Yunhai , Liu Wenbin , Zhang Jinjing
Published: April 2024

With the rapid development of power grid, the structure and technology of the secondary system in substations are also constantly innovating. The new generation of intelligent substations has achieved online monitoring functions for secondary equipment, making some state variables of relay protection equipment become observable indicators. Based on this, this paper proposes a novel relay protection equipment status evaluation strategy. Firstly, considering the fuzziness and uncertainty of the boundary division of relay protection evaluation levels, a relay protection risk assessment method based on normal cloud model has been proposed. Then, due to the particularity of historical statistical data, a weight calculation method combining analytical hierarchy process (AHP) and entropy weight method is adopted to eliminate subjective factors in the weight calculation process. Meanwhile, the equipment operation risk level was determined by calculating the certainty of the corresponding evaluation level for each indicator. Finally, in case study, the proposed method is used to evaluate the status of relay protection equipment, and the feasibility and accuracy of this method are verified through the analysis of the results.
A DC fault current fast-computing method of MMC-HVDC grid with short circuit protection equipment
Authors: Zhang Xiong , Yang Xu , Zhuo Chaoran
Published: April 2024

The multi-terminal modular multi-level converter-based high voltage direct current (MMC-HVDC) grid with short circuit protection equipment (SCPE) is so complex that it is difficult to estimate its fault current and analyze the performance of SCPE by conventional time-domain numerical calculation method, it meets three big obstacles. This paper has made significant progress in overcoming these obstacles. 1). By applying the modern electrical circuit theory, a systematic formulation of the differential equation set for fault current calculation is developed to avoid a lot of complex and cumbersome matrix manual calculations. 2). A novel Y-Delta transformation in the s-domain is proposed to develop an eliminating virtual node approach for a complex MMC-HVDC grid, including the ring, radial, and hybrid topologies. 3). It is difficult to solve the equivalent circuit of MMC-HVDC grid with SCPE since SCPE is a time-variable-nonlinear circuit. A canonical voltage source model of SCPE is established to transform the time-variable-nonlinear circuit into a piecewise linear circuit. Based on the three significant progresses, a DC fault current fast-computing method of MMC-HVDC grid with SCPE is put forward to deal with all kinds of MMC-HVDC grids with several kinds of SCPEs. Then, the performance of several kinds of SCPE is analyzed and compared by this method. Consequently, the proposed DC fault current fast-computing method is a new powerful tool to estimate the fault current of MMC-HVDC grid and analyze the performance of SCPE.
Chemical oxygen demand and biochemical oxygen demand analysis of discharge waters from laundry industry: monitoring, temporal variability, and biodegradability
Authors: Lacalamita Dario , Mongioví Chiara , Crini Grégorio
Published: April 2024

In Europe, chemical oxygen demand (COD) and biochemical oxygen demand (BOD5) are used as organic indicators for the analytical characterization of laundry water. However, the values of these indicators are derived from one-off analytical measurements at a defined frequency. In addition, there are few data on the temporal variability of COD and BOD5 parameters and on water biodegradability beyond 5 days. In this work, samples of physicochemical treated wastewater were collected for 1 year and their content in COD and BOD5 measured, with BOD monitored over 35 days. The results showed that, for the 24 samples studied, the average and standard deviation values were 835 ± 176 mg/L for COD and 445 ± 114 mg/L for BOD5, indicating a biodegradability index of 2.0 ± 0.4. The interpretation of the data also indicated that the concentrations were close to emission limit value, particularly for BOD5. BOD21 values were particularly high compared to the initial COD, indicating the biodegradability of water. Laundry effluents were also rich in substances very quickly biodegradable, which has been demonstrated by the values of the ratio between BOD7 and BOD5. However, a significant proportion of surfactants is not degraded, as shown by measurements of total organic carbon and anionic surfactant analysis. The results of this study could help the industrial partner involved in our project to guide its choice of the complementary method to better treat wastewater and to reduce its organic load.
Biosensing circulating MicroRNAs in autoinflammatory skin diseases: Focus on Hidradenitis suppurativa
Authors: Moltrasio Chiara , Silva Carlos André , Tricarico Paola Maura , Marzano Angelo Valerio , Sueleman Muhammad , Crovella Sergio
Published: April 2024

MicroRNAs (miRNAs) play a crucial role in the early diagnosis of autoinflammatory diseases, with Hidradenitis Suppurativa (HS) being a notable example. HS, an autoinflammatory skin disease affecting the pilosebaceous unit, profoundly impacts patients’ quality of life. Its hidden nature, with insidious initial symptoms and patient reluctance to seek medical consultation, often leads to a diagnostic delay of up to 7 years. Recognizing the urgency for early diagnostic tools, recent research identified significant differences in circulating miRNA expression, including miR-24-1-5p, miR-146a-5p, miR26a-5p, miR-206, miR338-3p, and miR-338-5p, between HS patients and healthy controls. These miRNAs serve as potential biomarkers for earlier disease detection. Traditional molecular biology techniques, like reverse transcription quantitative-polymerase chain reaction (RT-qPCR), are employed for their detection using specific primers and probes. Alternatively, short peptides offer a versatile and effective means for capturing miRNAs, providing specificity, ease of synthesis, stability, and multiplexing potential. In this context, we present a computational simulation pipeline designed for crafting peptide sequences that can capture circulating miRNAs in the blood of patients with autoinflammatory skin diseases, including HS. This innovative approach aims to expedite early diagnosis and enhance therapeutic follow-up, addressing the critical need for timely intervention in HS and similar conditions.
New insights into Trypanosoma cruzi genetic diversity, and its influence on parasite biology and clinical outcomes
Authors: Silvestrini Marina Malheiros Araújo , Alessio Glaucia Diniz , Frias Bruna Estefânia Diniz , Sales Júnior Policarpo Ademar , Araújo Márcio Sobreira Silva , Silvestrini Carolina Malheiros Araújo , Brito Alvim de Melo Gustavo Eustáquio , Martins-Filho Olindo Assis , Teixeira-Carvalho Andréa , Martins Helen Rodrigues
Published: April 2024

Chagas disease, caused by Trypanosoma cruzi, remains a serious public health problem worldwide. The parasite was subdivided into six distinct genetic groups, called “discrete typing units” (DTUs), from TcI to TcVI. Several studies have indicated that the heterogeneity of T. cruzi species directly affects the diversity of clinical manifestations of Chagas disease, control, diagnosis performance, and susceptibility to treatment. Thus, this review aims to describe how T. cruzi genetic diversity influences the biology of the parasite and/or clinical parameters in humans. Regarding the geographic dispersion of T. cruzi, evident differences were observed in the distribution of DTUs in distinct areas. For example, TcII is the main DTU detected in Brazilian patients from the central and southeastern regions, where there are also registers of TcVI as a secondary T. cruzi DTU. An important aspect observed in previous studies is that the genetic variability of T. cruzi can impact parasite infectivity, reproduction, and differentiation in the vectors. It has been proposed that T. cruzi DTU influences the host immune response and affects disease progression. Genetic aspects of the parasite play an important role in determining which host tissues will be infected, thus heavily influencing Chagas disease’s pathogenesis. Several teams have investigated the correlation between T. cruzi DTU and the reactivation of Chagas disease. In agreement with these data, it is reasonable to suppose that the immunological condition of the patient, whether or not associated with the reactivation of the T. cruzi infection and the parasite strain, may have an important role in the pathogenesis of Chagas disease. In this context, understanding the genetics of T. cruzi and its biological and clinical implications will provide new knowledge that may contribute to additional strategies in the diagnosis and clinical outcome follow-up of patients with Chagas disease, in addition to the reactivation of immunocompromised patients infected with T. cruzi.
Causal relationship between the immune cells and ankylosing spondylitis: univariable, bidirectional, and multivariable Mendelian randomization
Authors: Qin Chaofan , Yu Qingshuai , Deng Zhongliang , Zhang You , Chen Mingxin , Wang Xin , Hu Tao , Lei Bo , Yan Zhengjian , Cheng Si
Published: April 2024

Background

Ankylosing spondylitis (AS) is an autoimmune disease that affects millions of individuals. Immune cells have been recognized as having a crucial role in the pathogenesis of AS. However, their relationship has not been fully explored.

Methods

We chose to employ Mendelian randomization (MR) to investigate the potential correlation between immune cells and AS. We sourced the data on immune cells from the latest genome-wide association studies (GWASs). We obtained data on AS from the FinnGen consortium. Our comprehensive univariable MR analysis covered 731 immune cells to explore its potential causal relationship with AS. The primary analysis method was inverse-variance weighted (IVW). Additionally, we used Cochran’s Q test and the MR-Egger intercept test to assess the presence of pleiotropy and heterogeneity. We examined whether our results could be influenced by individual single-nucleotide polymorphisms (SNPs) using the leave-one-out test. We conducted a bidirectional MR to investigate the reverse relationship. We also applied multivariable MR to decrease the potential influence between the immune cells.

Results

Overall, our univariable MR analysis revealed eight immune cells associated with AS. Among these, four immune cells contributed to an increased risk of AS, while four immune cells were identified as protective factors for AS. However, the Bonferroni test confirmed only one risk factor and one protective factor with a significance level of p < 6.84E−05. CD8 on effector memory CD8+ T cell could increase the risk of AS (p: 1.2302E−05, OR: 2.9871, 95%CI: 1.8289–4.8786). HLA DR on CD33dim HLA DR+ CD11b+ could decrease the risk of AS (p: 1.2301E−06, OR: 0.5446, 95%CI: 0.4260–0.6962). We also identified a bidirectional relationship between CD4 on CD39+ activated CD4 regulatory T cells and AS utilizing the bidirectional MR. To address potential confounding among immune cells, we employed multivariable MR analysis, which revealed that only one immune cell had an independent effect on AS. HLA DR on CD33dim HLA DR+ CD11b+ could decrease the risk of AS (p: 2.113E−06, OR: 0.0.5423, 95%CI: 0.4210–0.6983). Our findings were consistently stable and reliable.

Conclusions

Our findings indicated a potential link between immune cells and AS, which could provide a new idea for future research. Nevertheless, the specific underlying mechanisms require further exploration.
C-reactive protein lowers the serum level of IL-17, but not TNF-α, and decreases the incidence of collagen-induced arthritis in mice
Authors: Singh Sanjay K. , Prislovsky Amanda , Ngwa Donald N. , Munkhsaikhan Undral , Abidi Ammaar H. , Brand David D. , Agrawal Alok
Published: April 2024

The biosynthesis of C-reactive protein (CRP) in the liver is increased in inflammatory diseases including rheumatoid arthritis. Previously published data suggest a protective function of CRP in arthritis; however, the mechanism of action of CRP remains undefined. The aim of this study was to evaluate the effects of human CRP on the development of collagen-induced arthritis (CIA) in mice which is an animal model of autoimmune inflammatory arthritis. Two CRP species were employed: wild-type CRP which binds to aggregated IgG at acidic pH and a CRP mutant which binds to aggregated IgG at physiological pH. Ten CRP injections were given on alternate days during the development of CIA. Both wild-type and mutant CRP reduced the incidence of CIA, that is, reduced the number of mice developing CIA; however, CRP did not affect the severity of the disease in arthritic mice. The serum levels of IL-17, IL-6, TNF-α, IL-10, IL-2 and IL-1β were measured: both wild-type and mutant CRP decreased the level of IL-17 and IL-6 but not of TNF-α, IL-10, IL-2 and IL-1β. These data suggest that CRP recognizes and binds to immune complexes, although it was not clear whether CRP functioned in its native pentameric or in its structurally altered pentameric form in the CIA model. Consequently, ligand-complexed CRP, through an as-yet undefined mechanism, directly or indirectly, inhibits the production of IL-17 and eventually protects against the initiation of the development of arthritis. The data also suggest that IL-17, not TNF-α, is critical for the development of autoimmune inflammatory arthritis.
Molecular characterization, localization, and physiological roles of ITP and ITP-L in the mosquito, Aedes aegypti
Authors: Sajadi Farwa , Paluzzi Jean-Paul V.
Published: April 2024

The insect ion transport peptide (ITP) and its alternatively spliced variant, ITP-like peptide (ITP-L), belong to the crustacean hyperglycemic hormone family of peptides and are widely conserved among insect species. While limited, studies have characterized the ITP/ITP-L signaling system within insects, and putative functions including regulation of ion and fluid transport, ovarian maturation, and thirst/excretion have been proposed. Herein, we aimed to molecularly investigate Itp and Itp-l expression profiles in the mosquito, Aedes aegypti, examine peptide immunolocalization and distribution within the adult central nervous system, and elucidate physiological roles for these neuropeptides. Transcript expression profiles of both AedaeItp and AedaeItp-l revealed distinct enrichment patterns in adults, with AedaeItp expressed in the brain and AedaeItp-l expression predominantly within the abdominal ganglia. Immunohistochemical analysis within the central nervous system revealed expression of AedaeITP peptide in a number of cells in the brain and in the terminal ganglion. Comparatively, AedaeITP-L peptide was localized solely within the pre-terminal abdominal ganglia of the central nervous system. Interestingly, prolonged desiccation stress caused upregulation of AedaeItp and AedaeItp-l levels in adult mosquitoes, suggesting possible functional roles in water conservation and feeding-related activities. RNAi-mediated knockdown of AedaeItp caused an increase in urine excretion, while knockdown of both AedaeItp and AedaeItp-l reduced blood feeding and egg-laying in females as well as hindered egg viability, suggesting roles in reproductive physiology and behavior. Altogether, this study identifies AedaeITP and AedaeITP-L as key pleiotropic hormones, regulating various critical physiological processes in the disease vector, A. aegypti.
Development of a multi-layer network model for characterizing energy cascade behavior on turbulent mixing
Authors: Mao Beibei , Yang Hua , Song Dalei , Li Junyang , Sun Weicheng , Liu Xiuyan
Published: April 2024

Eddies of various sizes are visible to the naked eye in turbulent flow. Each eddy scale corresponds to a fraction of the total energy released by the turbulence cascade. Understanding the dynamic mechanism of the energy cascade is crucial to the study of turbulent mixing. In this paper, an energy cascade multi-layer network (ECMN) based on the complex network algorithm is proposed to investigate the spatio-temporal evolution of the energy cascade, covering both the inertial and dispersive ranges. The dynamic process of energy cascade is transformed into a topological structure based on the node definition and edge determination. The topological structure allows for the exploration of eddies interaction and chaotic energy transfer across scales. The model results show the intermittent and non-uniform nature of the energy cascade. Meanwhile, the scale gap found in the model verifies the fractal property of the energy evolution. We also found that scales of the generated eddies in energy cascade process are stochastic, and a synchronous energy cascade pattern is demonstrated according to the constructed framework. Furthermore, it provides a topological way to evaluate the contribution of large and small scale eddies. In addition, a network structure coefficient κ is proposed to evaluate the energy transfer strength. It agrees very well with the fluctuation of dissipation rates. All of this shows that the network model can effectively reveal the inhomogeneous properties of the energy cascade and quantify the turbulent mixing intensity based on the intermittent scale interaction. This also provides new insights into the study of fractal scales of nonlinear complex systems and the bridging of chaotic dynamics with topological frameworks.
Qualitative enquiry on factors affecting antibiotic prescribing at primary healthcare facilities in Addis Ababa, Ethiopia
Authors: Altaye Fikru Worku , Thupayagale-Tshweneagae Gloria , Mfidi Faniswa Honest
Published: April 2024

Background

The major driver of antibiotic resistance is the huge increase in antibiotic prescribing, especially in low- and middle-income countries.

Aim

This study aimed to explore factors affecting antibiotic prescribing at primary healthcare facilities in Addis Ababa, Ethiopia.

Methods

The study was part of a three-phased explanatory sequential mixed method design. The study was conducted in five primary healthcare facilities through in-depth interviews of 20 prescribers and 22 key informants using pre-prepared interview guides. The data were analysed through thematic content analysis by applying ATLAS.ti 9 software.

Results

The antibiotic prescribing decision of prescribers was shown to be affected by various factors. The factors related to prescribers include not updating oneself on antibiotic use and antibiotic resistance, not reviewing patient history, not considering the concerns related to antibiotic resistance during prescribing, and competency problems. The patient-related factors were low awareness about antibiotics, lack of respect for prescribers, and pressure on prescribers. The shortage of antibiotics and laboratory reagents, a lack of updated antimicrobial resistance information, patient load, inadequate capacity, private sector practice, inadequate follow-up and support, and health insurance membership were the system-related factors. Appropriate interventions should be designed and implemented to address the identified factors and improve the prescribing practice.
Functional gas exchange measures on 129Xe MRI and spectroscopy are associated with age, sex, and BMI in healthy subjects
Authors: Mummy David , Zhang Shuo , Bechtel Aryil , Lu Junlan , Mammarappallil Joseph , Leewiwatwong Suphachart , Costelle Anna , Swaminathan Aparna , Driehuys Bastiaan
Published: April 2024

Introduction

Hyperpolarized 129Xe MRI and spectroscopy is a rapidly growing technique for assessing lung function, with applications in a wide range of obstructive, restrictive, and pulmonary vascular disease. However, normal variations in 129Xe measures of gas exchange across healthy subjects are not well characterized, presenting an obstacle to differentiating disease processes from the consequences of expected physiological heterogeneity. Here, we use multivariate models to evaluate the role of age, sex, and BMI in a range of commonly used 129Xe measures of gas exchange.

Materials and methods

Healthy subjects (N = 40, 16F, age 44.3 ± 17.8 yrs., min-max 22–87 years) with no history of cardiopulmonary disease underwent 129Xe gas exchange MRI and spectroscopy. We used multivariate linear models to assess the associations of age, sex, and body mass index (BMI) with the RBC:Membrane (RBC:M), membrane to gas (Mem:Gas), and red blood cell to gas (RBC:Gas) ratios, as well as measurements of RBC oscillation amplitude and RBC chemical shift.

Results

Age, sex, and BMI were all significant covariates in the RBC:M model. Each additional 10 years of age was associated with a 0.05 decrease in RBC:M (p < 0.001), each additional 10 points of BMI was associated with a decrease of 0.07 (p = 0.02), and males were associated with a 0.17 higher RBC:M than females (p < 0.001). For Mem:Gas, male sex was associated with a decrease and BMI was associated with an increase. For RBC:Gas, age was associated with a decrease and male sex was associated with an increase. RBC oscillation amplitude increased with age and RBC chemical shift was not associated with any of the three covariates.

Discussion

129Xe MRI and spectroscopy measurements in healthy subjects, particularly the widely used RBC:M measurement, exhibit heterogeneity associated in part with variations in subject age, sex, and BMI. Elucidating the contributions of these and other factors to 129Xe gas exchange measurements is a critical component for differentiating disease processes from expected variation in healthy subjects. Notably, the Mem:Gas and RBC chemical shift appear to be stable with aging, suggesting that unexplained deviations in these metrics may be signs of underlying abnormalities.
Knowledge, attitudes, and practices among Chinese reproductive-age women toward uterine adenomyosis
Authors: Ren Ruofei , Li Hongye , Zhang Jie , Li Xuhua , Yuan Liqing , Li Dongxiao , Shan Shuzhi , Shi Bin , Jiang Jing
Published: April 2024

Objective

This study aimed to assess the knowledge, attitudes and practices (KAP) among Chinese reproductive-age women toward uterine adenomyosis.

Methods

This web-based cross-sectional study was conducted between April 2023 and September 2023 at the Second Hospital of Hebei Medical University. A self-designed questionnaire was developed to collect demographic information of reproductive-age women, and assess their KAP toward uterine adenomyosis.

Results

A total of 520 valid questionnaires were collected. Among the participants, 127 (24.42%) were diagnosed with uterine adenomyosis, and 120 (23.08%) were accompanied by uterine fibroids. The mean knowledge, attitudes and practices scores were 3.54 ± 3.72 (possible range:0–10), 20.96 ± 3.19 (possible range:5–25) and 24.01 ± 4.95 (possible range:7–35), respectively. The structural equation model demonstrated that knowledge had direct effects on attitudes and practices, as indicated by a path coefficient of 0.714 (p < 0.001) and 1.510 (p < 0.001), respectively. Moreover, attitudes had direct effects on practices, with a path coefficient of 0.226 (p = 0.001).

Conclusion

The findings revealed that reproductive-age women have insufficient knowledge, negative attitudes, and poor practices toward the uterine adenomyosis. Comprehensive training programs are needed to improve reproductive-age women practices in this area.
Effects of coated sodium butyrate on the growth performance, serum biochemistry, antioxidant capacity, intestinal morphology, and intestinal microbiota of broiler chickens
Authors: Hou Jinwang , Lu Lizhi , Lian Lina , Tian Yong , Zeng Tao , Ma Yanfen , Li Sisi , Chen Li , Xu Wenwu , Gu Tiantian , Li Guoqin , Liu Xin
Published: April 2024

Introduction

This study examined the impact of adding coated sodium butyrate (CSB) to the diet on the growth performance, serum biochemistry, antioxidant capacity, intestinal morphology, and cecal microbiota of yellow-feathered broiler chickens.

Methods

In this study, 240 yellow-feathered broiler chickens at 26 days old were divided into two groups: the control group (CON group) received a standard diet, and the experimental group (CSB group) received a diet with 0.5 g/kg of a supplement called CSB. Each group had 6 replicates, with 20 chickens in each replicate, and the experiment lasted for 36 days.

Results

Compared to the CON group, the CSB group showed a slight but insignificant increase in average daily weight gain during the 26–62 day period, while feed intake significantly decreased. The CSB group exhibited significant increases in serum superoxide dismutase, catalase, and total antioxidant capacity. Additionally, the CSB group had significant increases in total protein and albumin content, as well as a significant decrease in blood ammonia levels. Compared to the CON group, the CSB group had significantly increased small intestine villus height and significantly decreased jejunal crypt depth. The abundance of Bacteroidetes and Bacteroides in the cecal microbiota of the CSB group was significantly higher than that of the CON group, while the abundance of Proteobacteria, Deferribacteres, and Epsilonbacteraeota was significantly lower than that of the CON group.

Conclusion

These results suggest that adding CSB to the diet can improve the growth performance and antioxidant capacity of yellow-feathered broiler chickens while maintaining intestinal health.
Innovative inbuilt moving bed biofilm reactor for nitrogen removal applied in household aquarium
Authors: Zhou Xiaolin , Liu Haicheng , Fan Xing , Xu Xinhao , Gao Yuan , Bi Xuejun , Cheng Lihua , Huang Shujuan , Zhao Fangchao , Yang Tang
Published: April 2024

An innovative inbuilt moving bed biofilm reactor (MBBR) was created to protect fish from nitrogen in a household aquarium. During the 90 experimental days, the ammonia nitrogen (NH4+-N) concentration in the aquarium with the inbuilt MBBR was always below 0.5 mg/L, which would not threaten the fish. Concurrently, nitrite and nitrate nitrogen concentrations were always below 0.05 mg/L and 4.5 mg/L, respectively. However, the blank contrast aquarium accumulated 1.985 mg/L NH4+-N on the 16th day, which caused the fish to die. The suspended biofilms could achieve the specific NH4+-N removal rate of 45.43 g/m3/d. Biofilms presented sparsely with filamentous structures and showed certain degrees of roughness. The bacterial communities of the suspended biofilms and the sediment were statistically different (p < 0.05), reflected in denitrifying and nitrifying bacteria. In particular, the relative abundance of Nitrospira reached 1.4%, while the genus was barely found in sediments. The suspended biofilms showed potentials for nitrification function with the predicted sequence numbers of ammonia monooxygenase [1.14.99.39] and hydroxylamine dehydrogenase [EC:1.7.2.6] of 220 and 221, while the values of the sediment were only 5 and 1. This study created an efficient NH4+-N removal inbuilt MBBR for household aquariums and explored its mechanism to afford a basis for its utilization.
From predator to protector: Myxococcus fulvus WCH05 emerges as a potent biocontrol agent for fire blight
Authors: Han Jian , Dong Zhiming , Ji Wenbo , Lv Wen , Luo Ming , Fu Benzhong
Published: April 2024

Fire blight, caused by the Gram-negative bacterium Erwinia amylovora, poses a substantial threat to pome fruit production worldwide. Despite existing control strategies, a pressing need remains for sustainable and environmentally friendly fire blight management. Myxobacteria, renowned for their predatory behavior and potent enzymes, emerge as a groundbreaking biocontrol approach with significant potential. Here, we report the biocontrol potential of a novel Myxococcus fulvus WCH05, against E. amylovora. Using various in vitro and planta assays, we demonstrated the multifaceted biocontrol abilities of strain WCH05. In plate predation assays, strain WCH05 exhibited not only strong predation against E. amylovora but also broad-spectrum activities against other plant pathogenic bacteria. Pre-treatment with strain WCH05 significantly decreased pear blossom blight incidence in detached inflorescence assays, achieving a controlled efficacy of 76.02% that rivaled the antibiotic streptomycin (79.79%). In greenhouse trials, strain WCH05 effectively reduced the wilting rate and disease index in young pear seedlings, exhibiting both protective (73.68%) and curative (68.66%) control. Further investigation revealed that the biocontrol activity of strain WCH05 relies on both direct contact and extracellular enzyme secretion. While cell extracts lacked inhibitory activity, ammonium sulfate-precipitated secreted proteins displayed potent lytic activity against E. amylovora. Substrate spectrum analysis identified peptidases, lipases, and glycosidases among the secreted enzymes, suggesting their potential roles in pathogen degradation and biocontrol efficacy. This study presents the first evidence of Myxococcus fulvus WCH05 as a biocontrol agent against fire blight. Its potent predatory abilities and enzymatic arsenal highlight its potential for sustainable disease management in pome fruit production. Future research will focus on identifying and characterizing specific lytic enzymes and optimizing strain WCH05 application strategies for field efficacy.
Photosynthetic response of Chlamydomonas reinhardtii and Chlamydomonas sp. 1710 to zinc toxicity
Authors: Zhan Di , Liu Yue , Yu Na , Hao Chunbo
Published: April 2024

Zinc (Zn) is an essential trace element but can lead to water contamination and ecological deterioration when present in excessive amounts. Therefore, investigating the photosynthetic response of microalgae to Zn stress is of great significance. In this study, we assessed the photosynthetic responses of neutrophilic Chlamydomonas reinhardtii and acidophilic Chlamydomonas sp. 1710 to Zn exposure for 96 h. The specific growth rate (μ), chlorophyll-a (Chl-a) content, and chlorophyll fluorescence parameters were determined. The results demonstrated that Chlamydomonas sp. 1710 was much more tolerant to Zn than C. reinhardtii, with the half-maximal inhibitory concentration (IC50) values of 225.4 mg/L and 23.4  mg/L, respectively. The μ and Chl-a content of C. reinhardtii decreased in the presence of 15  mg/L Zn, whereas those of Chlamydomonas sp. 1710 were unaffected by as high as 100  mg/L Zn. Chlorophyll fluorescence parameters indicated that the regulation of energy dissipation, including non-photochemical quenching, played a crucial role in Zn stress resistance for both Chlamydomonas strains. However, in the case of C. reinhardtii, non-photochemical quenching was inhibited by 5  mg/L Zn in the first 48 h, whereas for Chlamydomonas sp. 1710, it remained unaffected under 100  mg/L Zn. Chlamydomonas sp. 1710 also exhibited a 20 times stronger capacity for regulating the electron transfer rate than C. reinhardtii under Zn stress. The light energy utilization efficiency (α) of Chlamydomonas sp. 1710 had the most highly non-linear correlation with μ, indicating the energy utilization and regulation process of Chlamydomonas sp. 1710 was well protected under Zn stress. Collectively, our findings demonstrate that the photosystem of Chlamydomonas sp. 1710 is much more resilient and tolerant than that of C. reinhardtii under Zn stress.
B cells and the stressed brain: emerging evidence of neuroimmune interactions in the context of psychosocial stress and major depression
Authors: Engler-Chiurazzi Elizabeth
Published: April 2024

The immune system has emerged as a key regulator of central nervous system (CNS) function in health and in disease. Importantly, improved understanding of immune contributions to mood disorders has provided novel opportunities for the treatment of debilitating stress-related mental health conditions such as major depressive disorder (MDD). Yet, the impact to, and involvement of, B lymphocytes in the response to stress is not well-understood, leaving a fundamental gap in our knowledge underlying the immune theory of depression. Several emerging clinical and preclinical findings highlight pronounced consequences for B cells in stress and MDD and may indicate key roles for B cells in modulating mood. This review will describe the clinical and foundational observations implicating B cell-psychological stress interactions, discuss potential mechanisms by which B cells may impact brain function in the context of stress and mood disorders, describe research tools that support the investigation of their neurobiological impacts, and highlight remaining research questions. The goal here is for this discussion to illuminate both the scope and limitations of our current understanding regarding the role of B cells, stress, mood, and depression.
Understanding of facial features in face perception: insights from deep convolutional neural networks
Authors: Zhang Qianqian , Zhang Yueyi , Liu Ning , Sun Xiaoyan
Published: April 2024

Introduction

Face recognition has been a longstanding subject of interest in the fields of cognitive neuroscience and computer vision research. One key focus has been to understand the relative importance of different facial features in identifying individuals. Previous studies in humans have demonstrated the crucial role of eyebrows in face recognition, potentially even surpassing the importance of the eyes. However, eyebrows are not only vital for face recognition but also play a significant role in recognizing facial expressions and intentions, which might occur simultaneously and influence the face recognition process.

Methods

To address these challenges, our current study aimed to leverage the power of deep convolutional neural networks (DCNNs), an artificial face recognition system, which can be specifically tailored for face recognition tasks. In this study, we investigated the relative importance of various facial features in face recognition by selectively blocking feature information from the input to the DCNN. Additionally, we conducted experiments in which we systematically blurred the information related to eyebrows to varying degrees.

Results

Our findings aligned with previous human research, revealing that eyebrows are the most critical feature for face recognition, followed by eyes, mouth, and nose, in that order. The results demonstrated that the presence of eyebrows was more crucial than their specific high-frequency details, such as edges and textures, compared to other facial features, where the details also played a significant role. Furthermore, our results revealed that, unlike other facial features, the activation map indicated that the significance of eyebrows areas could not be readily adjusted to compensate for the absence of eyebrow information. This finding explains why masking eyebrows led to more significant deficits in face recognition performance. Additionally, we observed a synergistic relationship among facial features, providing evidence for holistic processing of faces within the DCNN.

Discussion

Overall, our study sheds light on the underlying mechanisms of face recognition and underscores the potential of using DCNNs as valuable tools for further exploration in this field.
A review of clinical use of surface-enhanced Raman scattering-based biosensing for glioma
Authors: Yang Guohui , Zhang Kaizhi , Xu Weiqing , Xu Shuping
Published: April 2024

Glioma is the most common malignant tumor of the nervous system in recent centuries, and the incidence rate of glioma is increasing year by year. Its invasive growth and malignant biological behaviors make it one of the most challenging malignant tumors. Maximizing the resection range (EOR) while minimizing the impact on normal brain tissue is crucial for patient prognosis. Changes in metabolites produced by tumor cells and their microenvironments might be important indicators. As a powerful spectroscopic technique, surface-enhanced Raman scattering (SERS) has many advantages, including ultra-high sensitivity, high specificity, and non-invasive features, which allow SERS technology to be widely applied in biomedicine, especially in the differential diagnosis of malignant tumor tissues. This review first introduced the clinical use of responsive SERS probes. Next, the sensing mechanisms of microenvironment-responsive SERS probes were summarized. Finally, the biomedical applications of these responsive SERS probes were listed in four sections, detecting tumor boundaries due to the changes of pH-responsive SERS probes, SERS probes to guide tumor resection, SERS for liquid biopsy to achieve early diagnosis of tumors, and the application of free-label SERS technology to detect fresh glioma specimens. Finally, the challenges and prospects of responsive SERS detections were summarized for clinical use.
Machine learning approach for ambient-light-corrected parameters and the Pupil Reactivity (PuRe) score in smartphone-based pupillometry
Authors: Bogucki Aleksander , John Ivo , Zinkiewicz Łukasz , Jachura Michał , Jaworski Damian , Suwała Karolina , Chrost Hugo , Wlodarski Michal , Kałużny Jakub , Campbell Doug , Bakken Paul , Pandya Shawna , Chrapkiewicz Radosław , Manohar Sanjay G.
Published: April 2024

Introduction

The pupillary light reflex (PLR) is the constriction of the pupil in response to light. The PLR in response to a pulse of light follows a complex waveform that can be characterized by several parameters. It is a sensitive marker of acute neurological deterioration, but is also sensitive to the background illumination in the environment in which it is measured. To detect a pathological change in the PLR, it is therefore necessary to separate the contributions of neuro-ophthalmic factors from ambient illumination. Illumination varies over several orders of magnitude and is difficult to control due to diurnal, seasonal, and location variations.

Methods and results

We assessed the sensitivity of seven PLR parameters to differences in ambient light, using a smartphone-based pupillometer (AI Pupillometer, Solvemed Inc.). Nine subjects underwent 345 measurements in ambient conditions ranging from complete darkness (<5 lx) to bright lighting (≲10,000 lx). Lighting most strongly affected the initial pupil size, constriction amplitude, and velocity. Nonlinear models were fitted to find the correction function that maximally stabilized PLR parameters across different ambient light levels. Next, we demonstrated that the lighting-corrected parameters still discriminated reactive from unreactive pupils. Ten patients underwent PLR testing in an ophthalmology outpatient clinic setting following the administration of tropicamide eye drops, which rendered the pupils unreactive. The parameters corrected for lighting were combined as predictors in a machine learning model to produce a scalar value, the Pupil Reactivity (PuRe) score, which quantifies Pupil Reactivity on a scale 0–5 (0, non-reactive pupil; 0–3, abnormal/“sluggish” response; 3–5, normal/brisk response). The score discriminated unreactive pupils with 100% accuracy and was stable under changes in ambient illumination across four orders of magnitude.

Discussion

This is the first time that a correction method has been proposed to effectively mitigate the confounding influence of ambient light on PLR measurements, which could improve the reliability of pupillometric parameters both in pre-hospital and inpatient care settings. In particular, the PuRe score offers a robust measure of Pupil Reactivity directly applicable to clinical practice. Importantly, the formulae behind the score are openly available for the benefit of the clinical research community.
The dilemma of neuroprotection trials in times of successful endovascular recanalization
Authors: Schmidt-Pogoda Antje , Kaesmacher Johannes , Bonberg Nadine , Werring Nils , Strecker Jan-Kolja , Koecke Mailin Hannah Marie , Beuker Carolin , Gralla Jan , Meier Raphael , Wiendl Heinz , Minnerup Heike , Fischer Urs , Minnerup Jens
Published: April 2024

Background

The “translational roadblock” between successful animal stroke studies and neutral clinical trials is usually attributed to conceptual weaknesses. However, we hypothesized that rodent studies cannot inform the human disease due to intrinsic pathophysiological differences between rodents and humans., i.e., differences in infarct evolution.

Methods

To verify our hypothesis, we employed a mixed study design and compared findings from meta-analyses of animal studies and a retrospective clinical cohort study. For animal data, we systematically searched pubmed to identify all rodent studies, in which stroke was induced by MCAO and at least two sequential MRI scans were performed for infarct volume assessment within the first two days. For clinical data, we included 107 consecutive stroke patients with large artery occlusion, who received MRI scans upon admission and one or two days later.

Results

Our preclinical meta-analyses included 50 studies with 676 animals. Untreated animals had a median post-reperfusion infarct volume growth of 74%. Neuroprotective treatments reduced this infarct volume growth to 23%. A retrospective clinical cohort study showed that stroke patients had a median infarct volume growth of only 2% after successful recanalization. Stroke patients with unsuccessful recanalization, by contrast, experienced a meaningful median infarct growth of 148%.

Conclusion

Our study shows that rodents have a significant post-reperfusion infarct growth, and that this post-reperfusion infarct growth is the target of neuroprotective treatments. Stroke patients with successful recanalization do not have such infarct growth and thus have no target for neuroprotection.