bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Batch Mass on Formation of NiTi Shape Memory Alloy Produced by High-Energy Ball Milling
Authors: Goryczka Tomasz , Salwa Piotr
Published: November 2021

A high-energy ball milling technique was used for production of the equiatomic NiTi alloy. The grinding batch was prepared in two quantities of 10 and 20 g. The alloy was produced using various grinding times. Scanning electron microscopy, X-ray diffraction, hardness measurement and differential scanning calorimetry were used for materials characterization at various milling stages. The produced alloy was studied by means of microstructure, chemical and phase composition, average grain and crystallite size, crystal lattice parameters and microstrains. Increasing the batch mass to 20 g and extending the grinding time to 140 h caused the increase in the average size of the agglomerates to 700 µm while the average crystallites size was reduced to a few nanometers. Microstrains were also reduced following elongation of milling time. Moreover, when the grinding time is extended, the amount of the monoclinic phase increases at the expense of the body-centered cubic one—precursors of crystalline, the B2 parent phase and the B19′ martensite. Crystallization takes place as a multistage process, however, at temperatures below 600 °C. After crystallization, the reversible martensitic transformation occurred with the highest enthalpy value—4 or 5 J/g after 120 and 140 h milling, respectively.
Recent Progress in Understanding the Nano/Micro-Mechanical Behavior of Austenite in Advanced High Strength Steels
Authors: Guan Qingwen , Lu Wenjun , He Binbin
Published: November 2021

Advanced high strength steels (AHSS) are developed to reduce vehicle weight without sacrificing passenger safety. The newly developed AHSS frequently incorporates the austenite as the intrinsic component with large amount and good stability, which is realized by carefully designed alloying elements and thermo-mechanical processing. To explore the great potential of austenite in enhancing the strain hardening behavior of AHSS, detailed information on the mechanical behavior of single austenite grain is a prerequisite, which can be collected by a small-scale test. The present work reviews the recent progress in understanding the nano/micro-mechanical behavior of austenite in varied AHSS. Three different plasticity modes including dislocation plasticity, martensitic transformation, and deformation twinning can be observed in the austenite grains during small-scale tests, given proper stacking fault energy and crystal orientation. The remaining issues concerned with the nano/micro-mechanical behavior of austenite are discussed. The present review advances the general understanding of the nano/micro-mechanical behavior of austenite grains in AHSS, which may shed light on the precise austenite engineering with the development of new AHSS, realizing the dream of high-performance steels at low cost.
Performance Assessment and Chip Morphology Evaluation of Austenitic Stainless Steel under Sustainable Machining Conditions
Authors: Das Anshuman , Padhan Smita , Das Sudhansu Ranjan , Alsoufi Mohammad S. , Ibrahim Ahmed Mohamed Mahmoud , Elsheikh Ammar
Published: November 2021

Sustainable manufacturing has received great attention in the last few decades for obtaining high quality products with minimal costs and minimal negative impacts on environment. Sustainable machining is one of the main sustainable manufacturing branches, which is concerned with improving environmental conditions, reducing power consumption, and minimizing machining costs. In the current study, the performance of three sustainable machining techniques, namely dry, compressed air cooling, and minimum quantity lubrication, is compared with conventional flood machining during the turning of austenitic stainless steel (Nitronic 60). This alloy is widely used in aerospace engine components, medical applications, gas power industries, and nuclear power systems due to its superior mechanical and thermal properties. Machining was performed using SiAlON ceramic tool with four different cutting speeds, feeds and a constant depth of cut. Consequently, various chip characteristics such as chip morphology, chip thickness, saw tooth distance and chip segmentation frequency were analyzed with both optical and scanning electron microscopes. Performance assessment was performed under the investigated cutting conditions. Our results show that the tool life under MQL machining are 138%, 72%, and 11% greater than dry, compressed air, and flooded conditions, respectively. The use of SiAlON ceramic tool results is more economically viable under the MQL environment as the overall machining cost per component is lower ($0.27) as compared to dry ($0.36), compressed air ($0.31), and flooded ($0.29) machining conditions. The minimum quantity lubrication technique outperformed the other investigated techniques in terms of eco-friendly aspects, economic feasibility, and technical viability to improve sustainability.
Review of the Recent Development in Metallic Glass and Its Composites
Authors: Sharma Adit , Zadorozhnyy Vladislav
Published: November 2021

Metallic glasses are known for their mechanical properties but lack plasticity. This could be prevented by combining them with other materials or by inducing a second phase to form a composite. These composites have enhanced thermo-physical properties. The review paper aims to outline a summary of the current research done on metallic glass and its composites. A background in the history, properties, and their applications is discussed. Recent developments in biocompatible metallic glass composites, fiber-reinforced metallic glass, ex situ and in situ, are discussed.
A Comparison of Methods for the Characterisation of Waste-Printed Circuit Boards
Authors: Van Yken Jonovan , Cheng Ka Yu , Boxall Naomi J. , Sheedy Chris , Nikoloski Aleksandar N. , Moheimani Navid R. , Kaksonen Anna H.
Published: November 2021

Electronic waste is a growing waste stream globally. With 54.6 million tons generated in 2019 worldwide and with an estimated value of USD 57 billion, it is often referred to as an urban mine. Printed circuit boards (PCBs) are a major component of electronic waste and are increasingly considered as a secondary resource for value recovery due to their high precious and base metals content. PCBs are highly heterogeneous and can vary significantly in composition depending on the original function. Currently, there are no standard methods for the characterisation of PCBs that could provide information relevant to value recovery operations. In this study, two pre-treatments, smelting and ashing of PCB samples, were investigated to determine the effect on PCB characterisation. In addition, to determine the effect of particle size and element-specific effects on the characterisation of PCBs, samples were processed using four different analytical methods. These included multi-acid digestion followed by inductively coupled plasma optical emission spectrometry (ICP-OES) analysis, nitric acid digestion followed by X-ray fluorescence (XRF) analysis, multi-acid digestion followed by fusion digestion and analysis using ICP-OES, and microwave-assisted multi-acid digestion followed by ICP-OES analysis. In addition, a mixed-metal standard was created to serve as a reference material to determine the accuracy of the various analytical methods. Smelting and ashing were examined as potential pre-treatments before analytical characterisation. Smelting was found to reduce the accuracy of further analysis due to the volatilisation of some metal species at high temperatures. Ashing was found to be a viable pre-treatment. Of the four analytical methods, microwave-assisted multi-acid digestion offered the most precision and accuracy. It was found that the selection of analytical methods can significantly affect the accuracy of the observed metal content of PCBs, highlighting the need for a standardised method and the use of certified reference material.
Thyroid Hormones, Peripheral White Blood Count, and Dose of Basal Insulin Are Associated with Changes in Nerve Conduction Studies in Adolescents with Type 1 Diabetes
Authors: Wysocka-Mincewicz Marta , Baszyńska-Wilk Marta , Mazur Maria , Byczyńska Aleksandra , Nowacka-Gotowiec Monika
Published: November 2021

Type 1 diabetes (T1D) in the child population is the third most common chronic disease. Diabetic peripheral neuropathy (DPN) is a very disabling and silently developing complication. This prospective, observational study enrolled 182 (93 girls) patients with T1D, aged 16.5–18 years. The aim of the study was to assess the correlation between factors of diabetes metabolic control, blood count, thyroid hormones, thyroid-stimulating hormone (TSH), level of cortisol, vitamin D3, metabolic factors, demographic data, and nerve conduction study (NCS) parameters. We revealed that in multivariate regression models for almost all NCS parameters, beside height and diabetes duration, significant factors were basal insulin dose per kilogram of weight (BID/kg), body mass index (BMI), and thyroid hormones. For conduction velocities of the motor nerves, mean HbA1c exists in models. In all models for all NCS parameters there exists at least one parameter of peripheral white blood cell counts (predominantly monocytes). There is a significant influence of thyroid hormones, peripheral blood white cells count, and BID per weight on parameters of NCS. It is essential to take care of the proper insulin dose per weight of patients and the adequate proportion of basal to prandial insulin.
Iron, Heme Synthesis and Erythropoietic Porphyrias: A Complex Interplay
Authors: Poli Antoine , Schmitt Caroline , Moulouel Boualem , Mirmiran Arienne , Puy Hervé , Lefèbvre Thibaud , Gouya Laurent
Published: November 2021

Erythropoietic porphyrias are caused by enzymatic dysfunctions in the heme biosynthetic pathway, resulting in porphyrins accumulation in red blood cells. The porphyrins deposition in tissues, including the skin, leads to photosensitivity that is present in all erythropoietic porphyrias. In the bone marrow, heme synthesis is mainly controlled by intracellular labile iron by post-transcriptional regulation: translation of ALAS2 mRNA, the first and rate-limiting enzyme of the pathway, is inhibited when iron availability is low. Moreover, it has been shown that the expression of ferrochelatase (FECH, an iron-sulfur cluster enzyme that inserts iron into protoporphyrin IX to form heme), is regulated by intracellular iron level. Accordingly, there is accumulating evidence that iron status can mitigate disease expression in patients with erythropoietic porphyrias. This article will review the available clinical data on how iron status can modify the symptoms of erythropoietic porphyrias. We will then review the modulation of heme biosynthesis pathway by iron availability in the erythron and its role in erythropoietic porphyrias physiopathology. Finally, we will summarize what is known of FECH interactions with other proteins involved in iron metabolism in the mitochondria.
Maternal High-Fat Feeding Affects the Liver and Thymus Metabolic Axis in the Offspring and Some Effects Are Attenuated by Maternal Diet Normalization in a Minipig Model
Authors: La Rosa Federica , Guiducci Letizia , Guzzardi Maria Angela , Cacciato Insilla Andrea , Burchielli Silvia , Brunetto Maurizia Rossana , Bonino Ferruccio , Campani Daniela , Iozzo Patricia
Published: November 2021

Maternal high-fat diet (HFD) affects metabolic and immune development. We aimed to characterize the effects of maternal HFD, and the subsequent diet-normalization of the mothers during a second pregnancy, on the liver and thymus metabolism in their offspring, in minipigs. Offspring born to high-fat (HFD) and normal diet (ND) fed mothers were studied at week 1 and months 1, 6, 12 of life. Liver and thymus glucose uptake (GU) was measured with positron emission tomography during hyperinsulinemic-isoglycemia. Histological analyses were performed to quantify liver steatosis, inflammation, and hepatic hematopoietic niches (HHN), and thymocyte size and density in a subset. The protocol was repeated after maternal-diet-normalization in the HFD group. At one week, HFDoff were characterized by hyperglycemia, hyperinsulinemia, severe insulin resistance (IR), and high liver and thymus GU, associating with thymocyte size and density, with elevated weight-gain, liver IR, and steatosis in the first 6 months of life. Maternal diet normalization reversed thymus and liver hypermetabolism, and increased HHN at one week. It also normalized systemic insulin-sensitivity and liver fat content at all ages. Instead, weight-gain excess, hyperglycemia, and hepatic IR were still observed at 1 month, i.e., end-lactation. We conclude that intra-uterine HFD exposure leads to time-changing metabolic and immune-correlated abnormalities. Maternal diet-normalization reversed most of the effects in the offspring.
The Application of Metabolomics in Forensic Science with Focus on Forensic Toxicology and Time-of-Death Estimation
Authors: Dawidowska Joanna , Krzyżanowska Marta , Markuszewski Michał Jan , Kaliszan Michał
Published: November 2021

Recently, the diagnostic methods used by scientists in forensic examinations have enormously expanded. Metabolomics provides an important contribution to analytical method development. The main purpose of this review was to investigate and summarize the most recent applications of metabolomics in forensic science. The primary research method was an extensive review of available international literature in PubMed. The keywords “forensic” and “metabolomics” were used as search criteria for the PubMed database scan. Most authors emphasized the analysis of different biological sample types using chromatography methods. The presented review is a summary of recently published implementations of metabolomics in forensic science and types of biological material used and techniques applied. Possible opportunities for valuable metabolomics’ applications are discussed to emphasize the essential necessities resulting in numerous nontargeted metabolomics’ assays.
The Linoleic Acid Content of the Stratum Corneum of Ichthyotic Golden Retriever Dogs Is Reduced as Compared to Healthy Dogs and a Significant Part Is Oxidized in Both Free and Esterified Forms
Authors: Popa Iuliana , Solgadi Audrey , Pin Didier , Watson Adrian L. , Haftek Marek , Portoukalian Jacques
Published: November 2021

Golden Retrievers may suffer from Pnpl1-related inherited ichthyosis. Our study shows that in the stratum corneum (SC) of ichthyotic dogs, linoleic acid (LA) is also present in the form of 9-keto-octadecadienoic acid (9-KODE) instead of the acylacid form as in normal dogs. The fatty acids purified from SC strips (LA, acylacids) were characterized by liquid chromatography-tandem mass spectrometry (LC-MS) and atmospheric pressure chemical ionization (APCI). Electrospray ionization (ESI) and MS2(MS/MS Tandem mass spectrum/spectra)/M3 (MS/MS/MS Tandem mass spectrum/spectra) fragmentation indicated the positions of the double bonds in 9-KODE. We showed that ichthyotic dogs have a threefold lower LA content in the form of acylacids. The MS2 fragmentation of acyl acids showed in some peaks the presenceof an ion at the m/z 279, instead of an ion at m/z 293 which is characteristic of LA. The detected variant was identified upon MS3 fragmentation as 9-keto-octadecadienoic acid (9-KODE), and the level of this keto-derivative was increased in ichthyotic dogs. We showed by the APCI that such keto forms of LA are produced from hydroperoxy-octadecadienoic acids (HpODE) upon dehydration. In conclusion, the free form of 9-KODE was detected in ichthyotic SC up to fivefold as compared to unaffected dogs, and analyses by HPLC (High performance liquid chromatography) and ESI-MS (Electrospray Ionization-Mass Spectrometry) indicated its production via dehydration of native 9-HpODE.
The Reciprocal Relationship between LDL Metabolism and Type 2 Diabetes Mellitus
Authors: Bonilha Isabella , Hajduch Eric , Luchiari Beatriz , Nadruz Wilson , Le Goff Wilfried , Sposito Andrei C.
Published: November 2021

Type 2 diabetes mellitus and insulin resistance feature substantial modifications of the lipoprotein profile, including a higher proportion of smaller and denser low-density lipoprotein (LDL) particles. In addition, qualitative changes occur in the composition and structure of LDL, including changes in electrophoretic mobility, enrichment of LDL with triglycerides and ceramides, prolonged retention of modified LDL in plasma, increased uptake by macrophages, and the formation of foam cells. These modifications affect LDL functions and favor an increased risk of cardiovascular disease in diabetic individuals. In this review, we discuss the main findings regarding the structural and functional changes in LDL particles in diabetes pathophysiology and therapeutic strategies targeting LDL in patients with diabetes.
Prognostic Value of Pretherapeutic Primary Tumor MTV from [18F]FDG PET in Radically Treated Cervical Cancer Patients
Authors: Cegla Paulina , Hofheinz Frank , Cholewiński Witold , Czepczyński Rafał , Kubiak Anna , van den Hoff Jörg , Boś-Liedke Agnieszka , Roszak Andrzej , Burchardt Ewa
Published: November 2021

The aim of this study was to assess the usefulness of pretherapeutic primary tumor metabolic tumor volume (MTV) in the prognosis of radically treated cervical cancer patients. Retrospective, single-centre analysis was performed on a group of 508 cervical cancer patients. All patients underwent a pretreatment [18F]FDG PET/CT study for the assessment of the disease stage. Several PET-derived parameters—namely, maximum standardized uptake value (SUVmax), mean standardized uptake value (SUVmean), total lesion glycolysis (TLG) and MTV, as well as the clinical parameters, were analysed in terms of the overall survival (OS), event-free survival (EFS), locoregional control (LRC) and freedom from distant metastases (FFDM). Hyperthermia and brachytherapy were prognostic for EFS, OS, and LRC.FIGO stage > II showed a significant effect on EFS, OS, and FFDM. Moreover, hysterectomy was prognostic for OS and histology was prognostic for FFDM. From the PET-derived parameters only MTV of the primary tumor had a significant influence on OS (cutoff point: >12.7 mL, HR: 2.8, 1.75–4.48 95% CI, p < 0.001), LRC (cutoff point: >13.7 mL, HR 2.82, 1.42–5.61 95% CI, p = 0.003), EFS (cutoff point: >10.4 mL, HR: 2.57, 1.67–3.97 95% CI, p < 0.001) and FFDM (cutoff point: >10.4 mL, HR: 5.04, 1.82–13.99 95% CI, p = 0.002). Pretreatment MTV from the primary tumor is the only independent prognostic parameter in OS, LRC, EFS, and FFDM in radically treated cervical cancer patients and should be used in clinical practice in assessing prognosis in these patients.
A Global Metabolic Map Defines the Effects of a Si-Based Biostimulant on Tomato Plants under Normal and Saline Conditions
Authors: Chele Kekeletso H. , Steenkamp Paul , Piater Lizelle A. , Dubery Ian A. , Huyser Johan , Tugizimana Fidele
Published: November 2021

The ongoing unpredictability of climate changes is exponentially exerting a negative impact on crop production, further aggravating detrimental abiotic stress effects. Several research studies have been focused on the genetic modification of crop plants to achieve more crop resilience against such stress factors; however, there has been a paradigm shift in modern agriculture focusing on more organic, eco-friendly and long-lasting systems to improve crop yield. As such, extensive research into the use of microbial and nonmicrobial biostimulants has been at the core of agricultural studies to improve crop growth and development, as well as to attain tolerance against several biotic and abiotic stresses. However, the molecular mechanisms underlying the biostimulant activity remain enigmatic. Thus, this study is a liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics approach to unravel the hypothetical biochemical framework underlying effects of a nonmicrobial biostimulant (a silicon-based formulation) on tomato plants (Solanum lycopersium) under salinity stress conditions. This metabolomics study postulates that Si-based biostimulants could alleviate salinity stress in tomato plants through modulation of the primary metabolism involving changes in the tricarboxylic acid cycle, fatty acid and numerous amino acid biosynthesis pathways, with further reprogramming of several secondary metabolism pathways such as the phenylpropanoid pathway, flavonoid biosynthesis pathways including flavone and flavanol biosynthesis. Thus, the postulated hypothetical framework, describing biostimulant-induced metabolic events in tomato plants, provides actionable knowledge necessary for industries and farmers to, confidently and innovatively, explore, design, and fully implement Si-based formulations and strategies into agronomic practices for sustainable agriculture and food production.
Plasma Metabolome Normalization in Rheumatoid Arthritis Following Initiation of Methotrexate and the Identification of Metabolic Biomarkers of Efficacy
Authors: Medcalf Matthew R. , Bhadbhade Pooja , Mikuls Ted R. , O’Dell James R. , Gundry Rebekah L. , Funk Ryan S.
Published: November 2021

Methotrexate (MTX) efficacy in the treatment of rheumatoid arthritis (RA) is variable and unpredictable, resulting in a need to identify biomarkers to guide drug therapy. This study evaluates changes in the plasma metabolome associated with response to MTX in RA with the goal of understanding the metabolic basis for MTX efficacy towards the identification of potential metabolic biomarkers of MTX response. Plasma samples were collected from healthy control subjects (n = 20), and RA patients initiating MTX therapy (n = 20, 15 mg/week) before and after 16 weeks of treatment. The samples were analyzed by a semi-targeted metabolomic analysis, and then analyzed by univariate and multivariate methods, as well as an enrichment analysis. An MTX response was defined as a clinically significant reduction in the disease activity score in 28 joints (DAS-28) of greater than 1.2; achievement of clinical remission, defined as a DAS-28 < 2.6, was also utilized as an additional measure of response. In this study, RA is associated with an altered plasma metabolome that is normalized following initiation of MTX therapy. Metabolite classes found to be altered in RA and corrected by MTX therapy were diverse and included triglycerides (p = 1.1 × 10−16), fatty acids (p = 8.0 × 10−12), and ceramides (p = 9.8 × 10−13). Stratification based on responses to MTX identified various metabolites differentially impacted in responders and non-responders including glucosylceramides (GlcCer), phosphatidylcholines (PC), sphingomyelins (SM), phosphatidylethanolamines (PE), choline, inosine, hypoxanthine, guanosine, nicotinamide, and itaconic acid (p < 0.05). In conclusion, RA is associated with significant alterations to the plasma metabolome displaying at least partial normalization following 16 weeks of MTX therapy. Changes in multiple metabolites were found to be associated with MTX efficacy, including metabolites involved in fatty acid/lipid, nucleotide, and energy metabolism.
Nanoresonator Enhancement of Majorana-Fermion-Induced Slow Light in Superconducting Iron Chains
Authors: Chen Huajun
Published: November 2021

We theoretically investigate Fano resonance in the absorption spectrum of a quantum dot (QD) based on a hybrid QD-nanomechanical resonator (QD–NR) system mediated by Majorana fermions (MFs) in superconducting iron (Fe) chains. The absorption spectra exhibit a series of asymmetric Fano line shapes, which are accompanied by the rapid normal phase dispersion and induce the optical propagation properties such as the slow light effect under suitable parametric regimes. The results indicated that the slow light induced by MFs can be obtained under different coupling regimes and different detuning regimes. Moreover, we also investigated the role of the NR, and the NR behaving as a phonon cavity enhances the slow light effect.
An N-Port Universal Multimode Optical Router Supporting Mode-Division Multiplexing
Authors: Yang Yuehong , Su Ye , Liu Bocheng , Chai Junxiong , Dai Li , Jiang Xiao , Ye Yichen , Song Tingting , Xie Yiyuan
Published: November 2021

Optical network-on-chip (ONoC) is based on optical interconnects and optical routers (ORs), which have obvious advantages in bandwidth and power consumption. Transmission capacity is a significant performance in ONoC architecture, which has to be fully considered during the design process. Relying on mode-division multiplexing (MDM) technology, the system capacity of optical interconnection is greatly improved compared to the traditional multiplexing technology. With the explosion in MDM technology, the optical router supporting MDM came into being. In this paper, we design a multimode optical router (MDM-OR) model and analyze its indicators. Above all, we propose a novel multimode switching element and design an N-port universal multimode optical router (MDM-OR) model. Secondly, we analyze the insertion loss model of different optical devices and the crosstalk noise model of N-port MDM-OR. On this basis, a multimode router structure of a single-mode five-port optical router is proposed. At the same time, we analyze the transmission loss, crosstalk noise, signal-to-noise radio (OSNR), and bit error rate (BER) of different input–output pairs by inputting the 1550 nm TE0, TE1, and TE2 modes to the router.
Combination of Ceramic Laser Micromachining and Printed Technology as a Way for Rapid Prototyping Semiconductor Gas Sensors
Authors: Samotaev Nikolay , Oblov Konstantin , Dzhumaev Pavel , Fritsch Marco , Mosch Sindy , Vinnichenko Mykola , Trofimenko Nikolai , Baumgärtner Christoph , Fuchs Franz-Martin , Wissmeier Lena
Published: November 2021

The work describes a fast and flexible micro/nano fabrication and manufacturing method for ceramic Micro-electromechanical systems (MEMS)sensors. Rapid prototyping techniques are demonstrated for metal oxide sensor fabrication in the form of a complete MEMS device, which could be used as a compact miniaturized surface mount devices package. Ceramic MEMS were fabricated by the laser micromilling of already pre-sintered monolithic materials. It has been demonstrated that it is possible to deposit metallization and sensor films by thick-film and thin-film methods on the manufactured ceramic product. The results of functional tests of such manufactured sensors are presented, demonstrating their full suitability for gas sensing application and indicating that the obtained parameters are at a level comparable to those of industrial produced sensors. Results of design and optimization principles of applied methods for micro- and nanosystems are discussed with regard to future, wider application in semiconductor gas sensors prototyping.
Non-Invasive Manipulation of Two-Phase Liquid–Liquid Slug Flow Parameters Using Magnetofluidics
Authors: Gladius Anoj , Höving Simon , Mendelawi Mehdy , Sreekumar Sheeba Harikrishna , Agar David
Published: November 2021

Liquid–liquid slug flow in a microcapillary, with its improved heat and mass transfer properties and narrow residence time, plays a vital role in process intensification. Knowledge of the flow properties in microchannels along variables’ controllability (e.g., phase ratio, slug length along with classical variables, such as pressure, temperature, and flow velocity) during operation is crucial. This work aids in this by using magnetofluidics to manipulate these parameters. A ferrofluid with reproducible properties is produced and, together with another phase, stable slug flow is generated. Micro-gear pumps and syringe pumps, with their traditional mechanical components, result in parts degrading over time due to fatigue caused by pressure differentials and corrosive chemicals. The microflow is also disturbed by the invasive nature of these pumps. A considerably energy-efficient, non-invasive alternative, with reduced mechanical interfacing is suggested in this work. It uses magnetic gradients to manipulate two-phase flow, one of which is a magnetically active phase. Conveying concepts using permanent magnets in the immediate vicinity of the flow are investigated. To operate this pump continuously and to be able to regulate the phase ratio, an electromagnetic non-invasive valve is developed. Phase separation is also carried out with an existing decanter design, modified using electromagnetism to work without a selective membrane, usually necessary for phase separation at this scale. This pump is then compared with similar pumps developed in the past.
Progressive Two-Stage Network for Low-Light Image Enhancement
Authors: Sun Yanpeng , Chang Zhanyou , Zhao Yong , Hua Zhengxu , Li Sirui
Published: November 2021

At night, visual quality is reduced due to insufficient illumination so that it is difficult to conduct high-level visual tasks effectively. Existing image enhancement methods only focus on brightness improvement, however, improving image quality in low-light environments still remains a challenging task. In order to overcome the limitations of existing enhancement algorithms with insufficient enhancement, a progressive two-stage image enhancement network is proposed in this paper. The low-light image enhancement problem is innovatively divided into two stages. The first stage of the network extracts the multi-scale features of the image through an encoder and decoder structure. The second stage of the network refines the results after enhancement to further improve output brightness. Experimental results and data analysis show that our method can achieve state-of-the-art performance on synthetic and real data sets, with both subjective and objective capability superior to other approaches.
Biomedical Microtechnologies Beyond Scholarly Impact
Authors: Vomero Maria , Schiavone Giuseppe
Published: November 2021

The recent tremendous advances in medical technology at the level of academic research have set high expectations for the clinical outcomes they promise to deliver. To the demise of patient hopes, however, the more disruptive and invasive a new technology is, the bigger the gap is separating the conceptualization of a medical device and its adoption into healthcare systems. When technology breakthroughs are reported in the biomedical scientific literature, news focus typically lies on medical implications rather than engineering progress, as the former are of higher appeal to a general readership. While successful therapy and diagnostics are indeed the ultimate goals, it is of equal importance to expose the engineering thinking needed to achieve such results and, critically, identify the challenges that still lie ahead. Here, we would like to provoke thoughts on the following questions, with particular focus on microfabricated medical devices: should research advancing the maturity and reliability of medical technology benefit from higher accessibility and visibility? How can the scientific community encourage and reward academic work on the overshadowed engineering aspects that will facilitate the evolution of laboratory samples into clinical devices?
Construction and Characterization of TiN/Si3N4 Composite Insulation Layer in TiN/Si3N4/Ni80Cr20 Thin Film Cutting Force Sensor
Authors: Ma Ruyuan , Wu Wenge , He Zhenyu , Cheng Yunping , Liu Lijuan , Zhao Yongjuan
Published: November 2021

The measurement of cutting force is an effective method for machining condition monitoring in intelligent manufacturing. Titanium nitride films and silicon nitride films were prepared on 304 stainless steel substrates by DC-reactive magnetron sputtering and plasma-enhanced chemical vapor deposition (PECVD). The effects of substrate negative bias and nitrogen flow on the surface microstructures of TiN film were investigated. The smoothness of the film is optimal when the bias voltage is −60 V. X-ray diffraction (XRD) analysis was performed on the samples with the optimal smoothness, and it was found that when the nitrogen flow rate was higher than 2 sccm, the titanium nitride film had a mixed phase of TiN(111) and (200). It is further revealed that the change of peak intensity of TiN(200) can be enhanced by nitrogen flow. Through atomic force microscopy (AFM), it is found that the stronger the intensity of the TiN (200) peak, the smoother the surface of the film is. Finally, the effect of different film thicknesses on the hardness and toughness of the TiN/Si3N4 film system was studied by nanoindentation experiments. The nanohardness (H) of the TiN/Si3N4 film can reach 39.2 GPa, the elastic modulus (E) is 480.4 GPa, the optimal toughness value (H3/E2) is 0.261 GPa, and the sample has good insulation performance. Linear fitting of the film’s toughness to nanohardness shows that TiN/Si3N4 films with higher hardness usually have a higher H3/E2 ratio.
Low Intensity Pulsed Ultrasound for Bone Tissue Engineering
Authors: McCarthy Colleen , Camci-Unal Gulden
Published: November 2021

As explained by Wolff’s law and the mechanostat hypothesis, mechanical stimulation can be used to promote bone formation. Low intensity pulsed ultrasound (LIPUS) is a source of mechanical stimulation that can activate the integrin/phosphatidylinositol 3-OH kinase/Akt pathway and upregulate osteogenic proteins through the production of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). This paper analyzes the results of in vitro and in vivo studies that have evaluated the effects of LIPUS on cell behavior within three-dimensional (3D) titanium, ceramic, and hydrogel scaffolds. We focus specifically on cell morphology and attachment, cell proliferation and viability, osteogenic differentiation, mineralization, bone volume, and osseointegration. As shown by upregulated levels of alkaline phosphatase and osteocalcin, increased mineral deposition, improved cell ingrowth, greater scaffold pore occupancy by bone tissue, and superior vascularization, LIPUS generally has a positive effect and promotes bone formation within engineered scaffolds. Additionally, LIPUS can have synergistic effects by producing the piezoelectric effect and enhancing the benefits of 3D hydrogel encapsulation, growth factor delivery, and scaffold modification. Additional research should be conducted to optimize the ultrasound parameters and evaluate the effects of LIPUS with other types of scaffold materials and cell types.
Effects of Additives on Silage Fermentation Characteristic and In Vitro Digestibility of Perennial Oat at Different Maturity Stages on the Qinghai Tibetan
Authors: Li Ping , Tang Xiaolong , Liao Chaosheng , Li Maoya , Chen Liangyin , Lu Guangrou , Huang Xiaokang , Chen Chao , Gou Wenlong
Published: November 2021

To effectively use local grass resources to cover the winter feed shortage on the Qinghai-Tibetan Plateau, the silage fermentation and in vitro digestibility of perennial oat (Helictotrichonvirescens Henr.) were investigated. Perennial oat was harvested at the heading/flowering stage, wilted under sunny conditions, chopped, vacuumed in small bag silos, and stored at ambient temperatures (5–15 °C) for 60 days. The silages were treated without (CK) or with local lactic acid bacteria (LAB) inoculant (IN1), commercial LAB inoculant (IN2), and sodium benzoate (BL). Control silages of perennial oat at early heading stage showed higher (p < 0.05) lactate and acetate contents and lower (p < 0.05) final pH, butyrate, and ammonia-N contents than those at the flowering stage. High levels of dry matter recovery (DMR) and crude protein (CP) were observed in IN1- and BL-treated silages, with high in vitro gas production and dry matter digestibility. Compared to CK, additives increased (p < 0.05) aerobic stability by inhibiting yeasts, aerobic bacteria, and coliform bacteria during ensiling. In particular, the local LAB inoculant increased (p < 0.05) concentrations of lactate, acetate and propionate, and decreased concentrations of butyrate and ammonia-N in silages. This study confirmed that local LAB inoculant could improve the silage quality of perennial oat, and this could be a potential winter feed for animals such as yaks on the Qinghai Tibetan Plateau.
The Effect of Lactiplantibacillus plantarum BX62 Alone or in Combination with Chitosan on the Qualitative Characteristics of Fresh-Cut Apples during Cold Storage
Authors: Zhao Qian , Tang Shihua , Fang Xiang , Wang Zhuo , Jiang Yu , Guo Xusheng , Zhu Jianning , Zhang Ying
Published: November 2021

In order to explore whether beneficial lactic acid bacteria (LAB) could prolong the shelf life and improve the quality of fresh-cut apples, Lactiplantibacillus plantarum BX62, which was isolated from traditional fermented yak yogurt, and chitosan (CT), were applied to fresh-cut apples, subsequently stored at 4 °C. On days 0, 2, 4, 6, and 8, apple slices were taken for physicochemical, microbiological analysis, and sensory evaluation. The results showed that apple slices coated with L. plantarum BX62 (8 log CFU/mL) presented lower weight loss and browning rate, higher DPPH scavenging capacity, and achieved more effective inhibition of polyphenol oxidase (PPO) and peroxidase (POD) activities compared to the control samples. The application of CT alone or in combination with L. plantarum BX62 resulted in a significant reduction in aerobic mesophilic bacteria (AMB), aerobic psychrophilic bacterial (APB), yeast and molds (YAMs) counts (2.31 log CFU/g for AMB, 2.55 for APB, and 1.58 for YAMs). In addition, L. plantarum BX62 coated apples showed acceptable sensory properties in terms of color, flavor, taste, texture, and overall visual quality during 8 d of storage. On this basis, L. plantarum BX62 could be used as an excellent bio-preservative to extend the shelf life and improve the quality of fresh-cut apples.
Combined Therapy with microRNA-Expressing Salmonella and Irradiation in Melanoma
Authors: Yoon Wonsuck , Park Yongsung , Kim Seunghyun , Park Yongkeun , Kim Chul Yong
Published: November 2021

Anticancer treatment strategies using bacteria as a vector are currently expanding with the development of anticancer drugs. Here, we present a research strategy to develop anticancer drugs using bacteria that contain miRNAs. We also present a strategy for the development of novel bacterial anticancer drugs in combination with radiation. Salmonella strains expressing miRNA were produced by modifying the miRNA expression vector encoding INHA, a radiation-resistant gene developed previously. The anticancer effect of INHA was confirmed using skin cancer cell lines. We also tested a combination strategy comprising bacteria and radiation for its anticancer efficacy against radiation-resistant mouse melanoma to increase the efficacy of radiation therapy as a novel strategy. The recombinant strain was confirmed to promote effective cell death even when combined with radiation therapy, which exerts its cytotoxicity by enhancing reactive oxygen species production. Moreover, a combination of bacterial and radiation therapy enhanced radiotherapy efficacy. When combined with radiation therapy, bacterial therapy exhibited effective anti-cancer properties even when administered to animals harboring radiation-resistant tumors. This strategy may promote the secretion of cytokines in cells and more effectively reduce the number of bacteria remaining in the animal. Thus, this study may lead to the development of a strategy to improve the effectiveness of radiation therapy using Salmonella expressing cancer-specific miRNA for intractable cancers such as those resistant to radiation.
Comparative Performances of Beneficial Microorganisms on the Induction of Durum Wheat Tolerance to Fusarium Head Blight
Authors: Kthiri Zayneb , Jabeur Maissa Ben , Harbaoui Kalthoum , Karmous Chahine , Chamekh Zoubeir , Chairi Fadia , Serret Maria Dolores , Araus Jose Luis , Hamada Walid
Published: November 2021

Durum wheat production is seriously threatened by Fusarium head blight (FHB) attacks in Tunisia, and the seed coating by bio-agents is a great alternative for chemical disease control. This study focuses on evaluating, under field conditions, the effect of seed coating with Trichoderma harzianum, Meyerozyma guilliermondii and their combination on (i) FHB severity, durum wheat grain yield and TKW in three crop seasons, and (ii) on physiological parameters and the carbon and nitrogen content and isotope composition in leaves and grains of durum wheat. The results indicated that the treatments were effective in reducing FHB severity by 30 to 70% and increasing grain yield with an increased rate ranging from 25 to 68%, compared to the inoculated control. The impact of treatments on grain yield improvement was associated with higher NDVI and chlorophyll content and lower canopy temperature. Furthermore, the treatments mitigated the FHB adverse effects on N and C metabolism by resulting in a higher δ13Cgrain (13C/12Cgrain) and δ15Ngrain (15N/14Ngrain). Overall, the combination outperformed the other seed treatments by producing the highest grain yield and TKW. The high potency of seed coating with the combination suggests that the two microorganisms have synergetic or complementary impacts on wheat.
The Role of Ticks in the Emergence of Borrelia burgdorferi as a Zoonotic Pathogen and Its Vector Control: A Global Systemic Review
Authors: Hussain Sabir , Hussain Abrar , Aziz Umair , Song Baolin , Zeb Jehan , George David , Li Jun , Sparagano Olivier
Published: November 2021

Ticks are widely distributed across the globe, serving as hosts for numerous pathogens that make them major contributors to zoonotic parasitosis. Borrelia burgdorferi is a bacterial species that causes an emerging zoonotic tick-borne disease known as Lyme borreliosis. The role of ticks in the transmission of this pathogen was explored in this study. According to this systematic review, undertaken according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, 19 tick species are known to carry Borrelia burgdorferi, with more than half of the recorded cases in the last two decades related to Ixodes ricinus and Ixodes scapularis ticks. Forty-six studies from four continents, Europe, North America, Asia, and Africa, reported this pathogen in ticks collected from vegetation, animals, and humans. This study highlights an increasing distribution of tick-associated Borrelia burgdorferi, likely driven by accelerated tick population increases in response to climate change coupled with tick dispersal via migratory birds. This updated catalogue helps in compiling all tick species responsible for the transmission of B. burgdorferi across the globe. Gaps in research exist on Borrelia burgdorferi in continents such as Asia and Africa, and in considering environmentally friendly vector control strategies in Europe and North America.
Down-Regulation of Yeast Helicase Ded1 by Glucose Starvation or Heat-Shock Differentially Impairs Translation of Ded1-Dependent mRNAs
Authors: Sen Neelam Dabas , Zhang Hongen , Hinnebusch Alan G.
Published: November 2021

Ded1 is an essential DEAD-box helicase in yeast that broadly stimulates translation initiation and is critical for mRNAs with structured 5′UTRs. Recent evidence suggests that the condensation of Ded1 in mRNA granules down-regulates Ded1 function during heat-shock and glucose starvation. We examined this hypothesis by determining the overlap between mRNAs whose relative translational efficiencies (TEs), as determined by ribosomal profiling, were diminished in either stressed WT cells or in ded1 mutants examined in non-stress conditions. Only subsets of the Ded1-hyperdependent mRNAs identified in ded1 mutant cells exhibited strong TE reductions in glucose-starved or heat-shocked WT cells, and those down-regulated by glucose starvation also exhibited hyper-dependence on initiation factor eIF4B, and to a lesser extent eIF4A, for efficient translation in non-stressed cells. These findings are consistent with recent proposals that the dissociation of Ded1 from mRNA 5′UTRs and the condensation of Ded1 contribute to reduced Ded1 function during stress, and they further suggest that the down-regulation of eIF4B and eIF4A functions also contributes to the translational impairment of a select group of Ded1 mRNA targets with heightened dependence on all three factors during glucose starvation.
A Mycobacteriophage-Based Vaccine Platform: SARS-CoV-2 Antigen Expression and Display
Authors: Freeman Krista G. , Wetzel Katherine S. , Zhang Yu , Zack Kira M. , Jacobs-Sera Deborah , Walters Sara M. , Barbeau Dominique J. , McElroy Anita K. , Williams John V. , Hatfull Graham F.
Published: November 2021

The explosion of SARS-CoV-2 infections in 2020 prompted a flurry of activity in vaccine development and exploration of various vaccine platforms, some well-established and some new. Phage-based vaccines were described previously, and we explored the possibility of using mycobacteriophages as a platform for displaying antigens of SARS-CoV-2 or other infectious agents. The potential advantages of using mycobacteriophages are that a large and diverse variety of them have been described and genomically characterized, engineering tools are available, and there is the capacity to display up to 700 antigen copies on a single particle approximately 100 nm in size. The phage body may itself be a good adjuvant, and the phages can be propagated easily, cheaply, and to high purity. Furthermore, the recent use of these phages therapeutically, including by intravenous administration, suggests an excellent safety profile, although efficacy can be restricted by neutralizing antibodies. We describe here the potent immunogenicity of mycobacteriophage Bxb1, and Bxb1 recombinants displaying SARS-CoV-2 Spike protein antigens.
The Exploitation of a Hempseed Byproduct to Produce Flavorings and Healthy Food Ingredients by a Fermentation Process
Authors: Nissen Lorenzo , Casciano Flavia , Babini Elena , Gianotti Andrea
Published: November 2021

Following the One Health principles in food science, the challenge to valorize byproducts from the industrial sector is open. Hemp (Cannabis sativa subsp. sativa) is considered an important icon of sustainability and as an alternative food source. Hemp seed bran, in particular, is a byproduct of industrial hemp seed processing, which is not yet valorized. The success, and a wider market diffusion of hemp seed for food applications, is hindered by its unpleasant taste, which is produced by certain compounds that generally overwhelm the pleasant bouquet of the fresh product. This research concerns the exploration of hemp seed bran through fermentation using beneficial lactobacilli, focusing on the sensorial and bioactive traits of the products when they are subjected to bacterial transformation. By studying of the aromatic profile formation during the fermentation process the aim was to modulate it in order to reduce off-odors without affecting the presence of healthy volatile organic compounds (VOCs). Applying multivariate analyses, it was possible to target the contribution of processing parameters to the generation of flavoring and bioactive compounds. To conclude, the fermentation process proposed was able to reduce unpleasant VOCs, whilst at the same time keeping the healthy ones, and it also improved nutritional quality, depending on time and bacterial starters. The fermentation proposed was a sustainable biotechnological approach that fitted perfectly with the valorization of hemp byproducts from the perspective of a green-oriented industrial process that avoids synthetic masking agents.