bluuebunny/crossref_metadata_2025 huggingface dataset:
Occurrence and Population Density of the Endemic Species Cordulegaster buchholzi (Anisoptera: Cordulegastridae) on the Cyclades Islands in Greece
Authors: Holuša Otakar , Holušová Kateřina
Published: November 2023

Our research was focused on determining the geomorphological characteristics of streams, characteristics of sediment in streams, habitat, emergence sites and flight period. Larvae were recorded in 19 streams (altitude of 35–680 m a.s.l.), with an average minimum width of 44.2 cm, an average maximum width of 352.9 cm, an average minimum depth of 9 cm and an average maximum depth (in pools) of 55 cm, with an average stream gradient of 12 grades (range 0.6–45 grades). In terms of grain size, the sediment in these biotopes can be characterized as sandy gravel, medium-grained gravel with an admixture of fine sand and an admixture of coarse-grained gravel prevails (with dominancy of fraction 2–5 mm with a representation of 47%). The larval density reached 0.1–62.2 larvae per 1 m2 of suitable sediment. Exuviae (100 exuviae found in total) occurred at an average of 66 cm horizontal distance from the shore and an average vertical height of 124 cm above the ground. The average total distance of larval movement was 190 cm. The emergence site was categorized as larvae-dominated tree trunks (57% of cases), rocks (51%) and overhanging rocks (11%). The flight period was recorded from 17th May to 15th July (literary record—to 15th August) with peak flight activity noted in the third quarter of June. Considering the size of the area—extent of occurrence, the population of C. buchholzi is strongly threatened; according to the IUCN categories it should be classified as endangered (EN).
Analysis of Online Platforms’ Free Trial Strategies for Digital Content Subscription
Authors: Chen Li
Published: November 2023

Adopting subscription models has become a popular trend in e-commerce. Digital content platforms often use free trials to attract potential subscribers. However, rigorous research on platforms’ free trial strategies is lacking. Our paper aims to fill this gap using a horizontal duopoly framework. We investigate both the scenario of market symmetry and of market asymmetry. We study the benchmark case with no free trial for each scenario and derive equilibrium results with free trial promotion. We find that the platform will not benefit from free trials unless consumers’ learning rate exceeds a threshold. When a free trial is introduced in market symmetry, a higher rate of consumers’ learning leads to a higher price of subscription and a shorter free trial length. In addition, when the network effect is incorporated, the platform will introduce a lower subscription price and a shorter free trial length. Under market asymmetry, our results show that the platform faces a higher threshold of consumers’ learning rate. Additionally, it will offer a higher price and a longer period of free trial compared with those under the market symmetry scenario. Overall, our study provides useful managerial insights for online content platforms when considering their subscription strategies.
Child Heritage Speakers’ Overregularization of Spanish Past Participles
Authors: Baker Martínez Elisabeth , Shin Naomi
Published: November 2023

The current study investigated overregularization of Spanish irregular past participles (e.g., dicho ‘said’, regularized as decido) among 20 child heritage speakers of Spanish in New Mexico, ages 5;1–11;9. Overregularization occurs when a child produces an irregular form analogously to its regular counterpart (e.g., eated instead of ate). Typically, children first produce the irregular form and then, after they have learned a morphological pattern, they overapply it to the irregular form. Ultimately, children retreat from overregularization and once again produce the target irregular form. While there has been a wealth of studies on monolingual children’s overregularizations, very few have investigated this phenomenon in child heritage speakers, who may develop their grammars diversely due to their exposure to the heritage language. This study analyzed the impact of age, Spanish language experience, Spanish morphosyntax proficiency, and lexical frequency on overregularization among the 13/20 children who produced past participles (n = 233) in response to an elicited production task. Participles were overregularized at high rates (74%), resulting in forms like ponido (‘put’, cf: puesto). Results from a regression analysis indicate that overregularization was more likely among the younger children, the children with lower morphosyntax scores, and with lower-frequency participles. Further, an interaction between morphosyntax score and lexical frequency indicated that children with higher scores overregularized with lower frequency participles, but not higher frequency ones, whereas children with low scores overregularized with both low- and high-frequency forms. In summary, child heritage speakers overregularize Spanish past participles at high rates, and the retreat from overregularization is tied to overall grammatical development and lexical frequency, suggesting that the acquisition of irregular participles is dependent on experiencing multiple instances of the irregular verb form.
Thermal Study of Carbon-Fiber-Reinforced Polymer Composites Using Multiscale Modeling
Authors: Nasri Wiem , Driss Zied , Djebali Ridha , Lee Kyu-Yeon , Park Hyung-Ho , Bezazi Abderazak , Reis Paulo N. B.
Published: November 2023

The layered fibers of carbon-fiber-reinforced polymer (CFRP) composites exhibit low thermal conductivity (TC) throughout their thickness due to the poor TC of the polymeric resin. Improved heat transmission inside the hydrogen storage tank during the filling process can reduce further compression work, and improved heat insulation can minimize energy loss. Therefore, it is crucial to understand the thermal properties of composites. This paper reports the thermal behavior of plain-woven CFRP composite using simulation at the micro-, meso-, and macro-scales. The TC was predicted numerically and compared to experimental findings and analytical models. Good results were found. Using the approach of multi-scale modeling, a parametric study was carried out to analyze in depth the influence of certain variables on thermal properties. The study revealed that both fiber volume fraction and temperature significantly influenced the TC of the composite, with the interphase fiber/matrix thickness following closely in terms of impact. The matrix porosity was found to have a relatively slighter impact, particularly within the porosity range of 5 to 15%.
Interaction Mechanism Characterized by Bond Performance and Diffusion Performance between TiO2@LDO and Asphalt Based on Molecular Dynamics Simulation
Authors: Wu Jinting , Zhao Peirou , Wang Ping , Guo Yang , Sun Fei , Li Cheng
Published: November 2023

In order to study the interaction between composite photocatalytic material TiO2@LDO and matrix asphalt, the four-component 12 molecular structure model of 70# matrix asphalt was optimized by using software Materials Studio 2020, and its heterostructure with TiO2@LDO composite was modeled. The bonding performance between asphalt and composite photocatalytic material was analyzed by interface energization, and the diffusion performance between asphalt and composite photocatalytic material was analyzed from the perspectives of particle movement and Z-direction density. By changing the temperature and other parameters in the simulation process, the change in bonding strength between TiO2@LDO and asphalt was investigated. Through the calculation and analysis of interaction energy, it was found that the adsorption and bonding strength between asphalt and TiO2@LDO were the strongest at 40 °C. At the same time, the diffusion performance was studied, and it was found that the molecular diffusion distribution of TiO2@LDO was more extensive at 60 °C, which laid the foundation for further blending of asphalt and TiO2@LDO. The simulation results show that TiO2@LDO molecules have a certain attraction to asphalt molecules and can modify the matrix asphalt to some extent.
Predictive Modeling of Tensile Strength in Aluminum Alloys via Machine Learning
Authors: Fu Keya , Zhu Dexin , Zhang Yuqi , Zhang Cheng , Wang Xiaodong , Wang Changji , Jiang Tao , Mao Feng , Zhang Cheng , Meng Xiaobo , Yu Hua
Published: November 2023

Aluminum alloys are widely used due to their exceptional properties, but the systematic relationship between their grain size and their tensile strength has not been thoroughly explored in the literature. This study aims to fill this gap by compiling a comprehensive dataset and utilizing machine learning models that consider both the alloy composition and the grain size. A pivotal enhancement to this study was the integration of hardness as a feature variable, providing a more robust predictor of the tensile strength. The refined models demonstrated a marked improvement in predictive performance, with XGBoost exhibiting an R2 value of 0.914. Polynomial regression was also applied to derive a mathematical relationship between the tensile strength, alloy composition, and grain size, contributing to a more profound comprehension of these interdependencies. The improved methodology and analytical techniques, validated by the models’ enhanced accuracy, are not only relevant to aluminum alloys, but also hold promise for application to other material systems, potentially revolutionizing the prediction of material properties.
Electrospun PCL Wires Loaded with Vancomycin on Zirconium Substrate
Authors: Radu (Dusman) Ramona-Daniela , Voicu Manuela Elena , Prodana Mariana , Demetrescu Ioana , Anuta Valentina , Draganescu Doina
Published: November 2023

The current study presents research about electrodeposition in relation to electrospinning PCL wires on a Zr substrate and loading the coating with vancomycin. The structural composition of the coatings was investigated via FT-IR analysis. The morphology evaluated using scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy, for the composition (SEM-EDS), evidenced the presence of the polymer wires, with and without drug vancomycin loading. The wettability of the coatings was evaluated from the hydrophobic–hydrophilic point of view, and the characterization was completed with mechanical and electrochemical tests. All the electrochemical tests performed in simulated body fluid highlighted that PCL represents a barrier against corrosion processes. The quantitative method to evaluate the loading efficiency shows that almost 80% of the total loaded vancomycin is released within 144 h; after the initial burst at 24 h, a steady release of vancomycin is observed over 7 days. A kinetic model of the drug release was also constructed.
Study of the Structural, Morphological, Strength and Shielding Properties of CuBi2O4 Films Obtained by Electrochemical Synthesis
Authors: Kadyrzhanov Dauren B. , Kaliyekperov Malik E. , Idinov Medet T. , Kozlovskiy Artem L.
Published: November 2023

In this research, the formation processes of CuBi2O4 films were examined using atomic force microscopy, energy dispersive analysis and X-ray diffraction analysis methods. The films were synthesized through electrochemical deposition from sulfuric acid solutions at a potential difference of 3.5 V. The duration of film growth was set to between 10 and 90 min to assess the possibility of controlled film growth and preserve the stability of their structural properties during growth over an extended period. An analysis of the data obtained by X-ray diffraction revealed that the resulting film samples are highly ordered structures with a tetragonal CuBi2O4 phase. The results of the connection between the thickness of CuBi2O4 films and strength properties depending on the time of film deposition were obtained. The results of the shielding efficiency of low-energy γ-quanta using CuBi2O4 films were obtained.
Study of Structural, Compression, and Soft Magnetic Properties of Fe65Ni28Mn7 Alloy Prepared by Arc Melting, Mechanical Alloying, and Spark Plasma Sintering
Authors: Zaara Kaouther , Optasanu Virgil , Le Gallet Sophie , Escoda Lluisa , Saurina Joan , Bernard Frédéric , Khitouni Mohamed , Suñol Joan-Josep , Chemingui Mahmoud
Published: November 2023

Soft magnetic Fe65Ni28Mn7 (at. %) alloy was successfully synthesized by mechanical alloying and spark plasma sintering (SPS) and, in parallel, the same composition was prepared by arc melting (AM) for comparison. Several SPS conditions were tested. X-ray diffraction and scanning electron microscopy were used to investigate the structure, phase composition, and morphology of the samples. It was found that mechanical alloying produced BCC and FCC supersaturated solid solution after 130 h of milling, with a fine microstructure (i.e., crystallite size of 10 nm). Spark plasma sintering performed at 750 °C and 1000 °C under two pressures of 50 MPa and 75 MPa revealed stable FCC phases. A single FCC phase was observed after the arc melting synthesis. The magnetic properties of milled powders and solids obtained by AM and SPS were investigated. The specimen consolidated by SPS at 1000 °C under the pressure of 50 MPa exhibits soft magnetic behavior (coercivity 0.07 Oe), whereas the mechanically alloyed sample revealed hard magnetic behavior. The specimen consolidated at 750 °C under a pressure of 75 MPa showed a higher compressive strength of 1700 MPa and a Vickers hardness of 425 ± 18 HV. As a result, sintering at 750 °C/75 MPa can be utilized to enhance the mechanical properties, while those sintered at 1000 °C/50 MPa increase magnetic softness.
Clinical Outcomes of Percutaneous Left-Atrial Appendage Occlusion with Conscious Sedation without an Anesthesiologist on Site: Results from a Multicenter Study
Authors: Bianco Matteo , Visalli Andrea Carmelo , Tomassini Francesco , Biolè Carloalberto , Giacobbe Federico , Rolfo Cristina , Cerrato Enrico , Franzè Alfonso , Zanda Greca , Pavani Marco , Mousavi Amir Hassan , Gobello Giulia , Piedimonte Giulio , Destefanis Paola , Lazzero Maurizio , Palacio Restrepo Sara , Celentani Dario , Luciano Alessia , Tizzani Emanuele , Chinaglia Alessandra , Varbella Ferdinando
Published: November 2023

Background and Objectives: Percutaneous left-atrial appendage (LAA) occlusion is an important therapeutic option for preventing cardioembolic stroke in patients with non-valvular atrial fibrillation (AF) at high risk of thromboembolic events and with contraindications for oral anticoagulation (OAC). It is usually performed with transesophageal echocardiography (TOE) guidance under general anesthesia (GA). In this retrospective study, we present a multicenter experience of LAA occlusion performed with conscious sedation (CS) without an anesthesiologist on site. Materials and Methods: All the patients on the waiting list for LAA occlusion procedure at Infermi Hospital, Rivoli, and San Luigi Gonzaga University Hospital, Orbassano, from October 2018 to October 2022 were analyzed. All the procedures were performed with a Watchman/FLX LAA closure device under TOE and fluoroscopic guidance without an anesthesiologist on site. CS was performed with a combination of midazolam and fentanyl as needed. Results: One-hundred fifteen patients were included (age 76.4 ± 7.6 years, median CHA2DS2Vasc 4.4 ± 1.4). CS was performed using midazolam (mean dose 5.9 ± 2.1 mg), adding fentanyl for thirty-nine (33.9%) patients in case of poor tolerance for the procedure despite midazolam. The acute procedural success rate was 99.1%. We observed seven acute severe complications. No patients needed anesthesiological assistance during the procedure, and no cases of respiratory failure necessitating ventilation were reported. In a follow-up after 10 ± 9 months, one case of stroke (0.9%) and one case (0.9%) of transient ischemic attack (TIA) occurred. Conclusions: LAA occlusion performed under CS and without the presence of an anesthesiologist on site appears to be safe and effective. It can be an attractive alternative to general anesthesia (GA), as fewer resources are required.
Premenstrual Syndrome and Premenstrual Dysphoric Disorder’s Impact on Quality of Life, and the Role of Physical Activity
Authors: Liguori Fabrizio , Saraiello Emma , Calella Patrizia
Published: November 2023

Most women who menstruate experience various physical, psychological, and behavioral changes during the period between ovulation and menstruation. This study focuses on defining and diagnosing premenstrual disorders, distinguishing between premenstrual symptoms (PS), PMS, and premenstrual dysphoric disorder (PMDD). It highlights the prevalence of these conditions and their impact on women’s quality of life, including social, occupational, and psychological aspects. Furthermore, the study examines the role of physical activity, particularly aerobic exercise, in managing premenstrual symptoms. Several systematic reviews are cited, suggesting that regular physical activity can effectively reduce both physical and psychological symptoms associated with PMS. In conclusion, the management of PMS involves a multifaceted approach, with exercises, dietary modifications, stress management, cognitive-behavioral therapy, and medications all playing roles. Physical activity, especially aerobic exercise, has been found to be an effective non-pharmacological therapy for alleviating PMS symptoms and improving overall well-being. However, more research is needed to determine the optimal type and dosage of exercise for individual women with PMS.
Effect of Channel Shape on Performance of Printed Indium Gallium Zinc Oxide Thin-Film Transistors
Authors: Yan Xingzhen , Li Bo , Zhang Yiqiang , Wang Yanjie , Wang Chao , Chi Yaodan , Yang Xiaotian
Published: November 2023

Printing technology will improve the complexity and material waste of traditional deposition and lithography processes in device fabrication. In particular, the printing process can effectively control the functional layer stacking and channel shape in thin-film transistor (TFT) devices. We prepared the patterning indium gallium zinc oxide (IGZO) semiconductor layer with Ga, In, and Zn molar ratios of 1:2:7 on Si/SiO2 substrates. And the patterning source and drain electrodes were printed on the surface of semiconductor layers to construct a TFT device with the top contact and bottom gate structures. To overcome the problem of uniform distribution of applied voltages between electrode centers and edges, we investigated whether the circular arc channel could improve the carrier regulation ability under the field effect in printed TFTs compared with a traditional structure of rectangular symmetry and a rectangular groove channel. The drain current value of the IGZO TFT with a circular arc channel pattern was significantly enhanced compared to that of a TFT with rectangular symmetric source/drain electrodes under the corresponding drain–source voltage and gate voltage. The field effect properties of the device were obviously improved by introducing the arc-shaped channel structure.
Electrophoretic Deposition of Multi-Walled Carbon Nanotube Coatings on CoCrMo Alloy for Biomedical Applications
Authors: Łosiewicz Bożena , Osak Patrycja , Górka-Kulikowska Karolina
Published: November 2023

Carbon nanotubes are a promising material for use in innovative biomedical solutions due to their unique chemical, mechanical, electrical, and magnetic properties. This work provides a method for the development of ultrasonically assisted electrophoretic deposition of multi-walled carbon nanotubes on a CoCrMo dental alloy. Functionalization of multi-walled carbon nanotubes was carried out by chemical oxidation in a mixture of nitric and sulfuric acids. The modified and unmodified multi-walled carbon nanotubes were anaphoretically deposited on the CoCrMo alloy in an aqueous solution. Chemical composition was studied by Fourier transform infrared spectroscopy. Surface morphology was examined by scanning electron microscopy. The mechanism and kinetics of the electrochemical corrosion of the obtained coatings in artificial saliva at 37 °C were determined using the open-circuit potential method, electrochemical impedance spectroscopy, and anodic polarization curves. The capacitive behavior and high corrosion resistance of the tested electrodes were revealed. It was found that the kinetics of electrochemical corrosion of the CoCrMo electrode significantly decreased in the presence of the functionalized multi-walled carbon nanotube coating. Electrophoretic deposition was shown to be an effective, low-cost, and fast method of producing nanotubes with controlled thickness, homogeneity, and packing density.
Phase-Controlled Tunable Unconventional Photon Blockade in a Single-Atom-Cavity System
Authors: Li Hong , Liu Ming , Yang Feng , Zhang Siqi , Ruan Shengping
Published: November 2023

In the past few years, cavity optomechanical systems have received extensive attention and research and have achieved rapid development both theoretically and experimentally. The systems play an important role in many fields, such as quantum information processing, optomechanical storage, high-precision measurement, macroscopic entanglement, ultrasensitive sensors and so on. Photon manipulation has always been one of the key tasks in quantum information science and technology. Photon blockade is an important way to realize single photon sources and plays an important role in the field of quantum information. Due to the nonlinear coupling of the optical force system, the energy level is not harmonic, resulting in a photon blockade effect. In this paper, we study the phase-controlled tunable unconventional photon blockade in a single-atom-cavity system, and the second-order nonlinear crystals are attached to the cavity. The cavity interacts with squeezed light, which results in a nonlinear process. The system is driven by a complex pulsed laser, and the strength of the coherent driving contains the phase. We want to study the effect of squeezed light and phase. We use the second-order correlation function to numerically and theoretically analyze the photon blockade effect. We show that quantum interference of two-photon excitation between three different transition pathways can cause a photon blockade effect. When there is no squeezed light, the interference pathways becomes two, but there are still photon blockade effects. We explore the influence of the tunable phase and second-order nonlinear strength on the photon blockade effect. We calculate the correlation function and compare the numerical results with the analytical results under certain parameters and find that the agreement is better.
Metal Oxide Nanowire-Based Sensor Array for Hydrogen Detection
Authors: Zappa Dario , Kaur Navpreet , Moumen Abderrahim , Comini Elisabetta
Published: November 2023

Accurate hydrogen leakage detection is a major requirement for the safe and widespread integration of this fuel in modern energy production devices, such as fuel cells. Quasi-1D nanowires of seven different metal oxides (CuO, WO3, Nb-added WO3, SnO2, ZnO, α-Bi2O3, NiO) were integrated into a conductometric sensor array to evaluate the hydrogen-sensing performances in the presence of interfering gaseous compounds, namely carbon monoxide, nitrogen dioxide, methane, acetone, and ethanol, at different operating temperatures (200–400 °C). Principal component analysis (PCA) was applied to data extracted from the array, demonstrating the ability to discriminate hydrogen over other interferent compounds. Moreover, a reduced array formed by only five sensors is proposed. This compact array may be easily implementable into artificial olfaction systems used in real hydrogen detection applications.
Controllable Carrier Doping in Two-Dimensional Materials Using Electron-Beam Irradiation and Scalable Oxide Dielectrics
Authors: Wang Lu , Guo Zejing , Lan Qing , Song Wenqing , Zhong Zhipeng , Yang Kunlin , Zhao Tuoyu , Huang Hai , Zhang Cheng , Shi Wu
Published: November 2023

Two-dimensional (2D) materials, characterized by their atomically thin nature and exceptional properties, hold significant promise for future nano-electronic applications. The precise control of carrier density in these 2D materials is essential for enhancing performance and enabling complex device functionalities. In this study, we present an electron-beam (e-beam) doping approach to achieve controllable carrier doping effects in graphene and MoS2 field-effect transistors (FETs) by leveraging charge-trapping oxide dielectrics. By adding an atomic layer deposition (ALD)-grown Al2O3 dielectric layer on top of the SiO2/Si substrate, we demonstrate that controllable and reversible carrier doping effects can be effectively induced in graphene and MoS2 FETs through e-beam doping. This new device configuration establishes an oxide interface that enhances charge-trapping capabilities, enabling the effective induction of electron and hole doping beyond the SiO2 breakdown limit using high-energy e-beam irradiation. Importantly, these high doping effects exhibit non-volatility and robust stability in both vacuum and air environments for graphene FET devices. This methodology enhances carrier modulation capabilities in 2D materials and holds great potential for advancing the development of scalable 2D nano-devices.
Influence of Temperature and Incidence Angle on the Irradiation Cascade Effect of 6H-SiC: Molecular Dynamics Simulations
Authors: Chen Yaolin , Liu Hongxia , Yan Cong , Wei Hao
Published: November 2023

SiC devices have been typically subjected to extreme environments and complex stresses during operation, such as intense radiation and large diurnal amplitude differences on the lunar surface. Radiation displacement damage may lead to degradation or failure of the performance of semiconductor devices. In this paper, the effects of temperature and incidence angle on the irradiation cascade effect of 6H-SiC were investigated separately using the principles of molecular dynamics. Temperatures were set to 100 K, 150 K, 200 K, 250 K, 300 K, 350 K, 400 K and 450 K. The incidence direction was parallel to the specified crystal plane, with angles of 8°, 15°, 30°, 45°, 60° and 75° to the negative direction of the Z-axis. In this paper, the six types of defects were counted, and the microscopic distribution images and trajectories of each type of defect were extracted. The results show a linear relationship between the peak of the Frenkel pair and temperature. The recombination rate of Frenkel pairs depends on the local temperature and degree of aggregation at the center of the cascade collision. Increasing the angle of incidence first inhibits and then promotes the production of total defects and Frenkel pairs. The lowest number of total defects, Frenkel pairs and antisite defects are produced at a 45° incident angle. At an incidence angle of 75°, larger size hollow clusters and anti-clusters are more likely to appear in the 6H-SiC.
Broadband Continuous Transverse Stub (CTS) Array Antenna for High-Power Applications
Authors: Sun Yunfei , Zhou Kelin , He Juntao , Yang Zihan , Yuan Chengwei , Zhang Qiang
Published: November 2023

A continuous transverse stub (CTS) array antenna with broad bandwidth and high-power handling capacity is proposed in this paper. The technologies of multi-step impedance matching and T-shaped electromagnetic band-gap (EBG) loading are utilized, which improved the antenna operating frequency bandwidth. An H-plane lens horn is used to feed the CTS array. As a result, a good bandwidth capability of more than 32% is achieved, with a gain variation less than 3.0 dB. The measured sidelobe level (SLL) is below −18 dB in the entire frequency range. Moreover, the power handling capacity of the antenna is more than 80 MW and can reach the GW level after arraying, which indicates that this antenna has application potential in the high-power microwave (HPM) field.
Numerical Study on a Liquid Cooling Plate with a Double-Layer Minichannel for a Lithium Battery Module
Authors: Xu Yu , Wang Ruijin
Published: November 2023

The liquid cooling system of lithium battery modules (LBM) directly affects the safety, efficiency, and operational cost of lithium-ion batteries. To meet the requirements raised by a factory for the lithium battery module (LBM), a liquid cooling plate with a two-layer minichannel heat sink has been proposed to maintain temperature uniformity in the module and ensure it stays within the temperature limit. This innovative design features a single inlet and a single outlet. To evaluate the performance of the liquid cooling system, we considered various discharge rates while taking into account the structure, flow rate, and temperature of the coolant. Our findings indicate that at a mass outflow rate of 20 g/s, a better cooling effect and lower power consumption can be achieved. An inlet temperature of 20 °C, close to the initial temperature of the battery string, may be the most appropriate because a higher temperature of the coolant will cause a higher temperature of LBM, so far as to exceed the safe threshold value. In the case of larger rate discharge, the design of a double-layer MCHS at the bottom and an auxiliary one at the side can effectively reduce the maximum temperature LBM (within 28 °C) and maintain the temperature difference in the single cell at approximately 4 °C. In the case of non-constant discharges, the temperature difference between cells increases with the maximum temperature. When the discharge rate is reduced, the large temperature difference helps the temperature to drop rapidly. This can provide guidance for the design of cooling systems for the LBM.
Dietary Habits and Oral Hygiene as Determinants of the Incidence and Intensity of Dental Caries—A Pilot Study
Authors: Mazurkiewicz Dominika , Pustułka Malwina , Ambrozik-Haba Jagoda , Bienkiewicz Maciej
Published: November 2023

Objectives: The aim of this study was to assess the influence of dietary and hygiene habits on the prevalence and intensity of dental caries. A group of 148 adults participated in the study. Methods: A self-administered nutrition and oral hygiene questionnaire was used to assess dietary habits and oral hygiene routine. A preventive diet index (PDI), a cariogenic diet index (CDI), and an oral health hygiene and prevention index (OHHPI) were created based on part of the questions. The intensity of dental caries among the respondents was investigated by the decayed, missing, and filled teeth index (DMFT), which was estimated on the basis of data obtained during the dental examination. Results: The study showed that 97% of the respondents had filled carious cavities, while untreated carious cavities were observed in up to 78% of the study participants. The respondents had an average consumption of preventive products and a low consumption of caries-stimulating foods. The preventive dietary index (PDI) value was higher in the female group than in the male group. A more frequent consumption of caries-inhibiting products was demonstrated by those declaring that they took various types of dietary supplements. The use of health-promoting sugar substitutes by the respondents was associated with a lower intensity of dental caries and a more frequent consumption of preventive products. Conclusions: The analysis of the survey results indicates the need to implement educational activities aimed at increasing public awareness of the prevalence of dental caries among adults.
Compromised NHE8 Expression Is Responsible for Vitamin D-Deficiency Induced Intestinal Barrier Dysfunction
Authors: Guo Yaoyu , Li Yanni , Tang Zeya , Geng Chong , Xie Xiaoxi , Song Shuailing , Wang Chunhui , Li Xiao
Published: November 2023

Objectives: Vitamin D (VitD) and Vitamin D receptor (VDR) are suggested to play protective roles in the intestinal barrier in ulcerative colitis (UC). However, the underlying mechanisms remain elusive. Evidence demonstrates that Na+/H+ exchanger isoform 8 (NHE8, SLC9A8) is essential in maintaining intestinal homeostasis, regarded as a promising target for UC therapy. Thus, this study aims to investigate the effects of VitD/VDR on NHE8 in intestinal protection. Methods: VitD-deficient mice, VDR−/− mice and NHE8−/− mice were employed in this study. Colitis mice were established by supplementing DSS-containing water. Caco-2 cells and 3D-enteroids were used for in vitro studies. VDR siRNA (siVDR), VDR over-expression plasmid (pVDR), TNF-α and NF-κb p65 inhibitor QNZ were used for mechanical studies. The expression of interested proteins was detected by multiple techniques. Results: In colitis mice, paricalcitol upregulated NHE8 expression was accompanied by restoring colonic mucosal injury. In VitD-deficient and VDR−/− colitis mice, NHE8 expression was compromised with more serious mucosal damage. Noteworthily, paricalcitol could not prevent intestinal barrier dysfunction and histological destruction in NHE8−/− mice. In Caco-2 cells and enteroids, siVDR downregulated NHE8 expression, further promoted TNF-α-induced NHE8 downregulation and stimulated TNF-α-induced NF-κb p65 phosphorylation. Conversely, QNZ blocked TNF-α-induced NHE8 downregulation in the absence or presence of siVDR. Conclusions: Our study indicates depressed NHE8 expression is responsible for VitD-deficient-induced colitis aggravation. These findings provide novel insights into the molecular mechanisms of VitD/VDR in intestine protection in UC.
Effects of Diet Macronutrient Composition on Weight Loss during Caloric Restriction and Subsequent Weight Regain during Refeeding in Aging Mice
Authors: Minderis Petras , Fokin Andrej , Povilonis Tomas , Kvedaras Mindaugas , Ratkevicius Aivaras
Published: November 2023

Caloric restriction (CR) induces weight loss, but is associated with rapid weight regain upon return to ad libitum feeding. Our aim was to investigate effects of the macronutrient composition of the diet on weight loss and regain in elderly mice. Males, 18 months old, of the C57BL/6J strain were subjected to 4-week 30% CR followed by 4 weeks of ad libitum refeeding on either high-carb (HC), high-fat (HF) or high-protein (HP) diets (n = 22 each). Mice (n = 11) fed a chow diet ad libitum served as a control group (CON). Body mass and food intake were monitored daily. Twenty-four-hour indirect calorimetry was used to assess energy expenditure and substrate oxidation. Muscle and fat mass were evaluated with dissection of the tissues. Serum leptin and ghrelin levels were also measured. CR-induced weight loss did not differ between the diets. Weight regain was particularly fast for HF as mice overshot their initial weight by 12.8 ± 5.7% after 4-week refeeding when HC and HP mice reached the weight of the CON group. Weight regain strongly correlated with energy intake across the groups. The respiratory exchange ratio was lower in HF mice (0.81 ± 0.03) compared to HC (0.94 ± 0.06, p < 0.001), HP (0.89 ± 0.04, p < 0.001) and CON mice (0.91 ± 0.06, p < 0.01) during the refeeding. Serum leptin levels were higher in HF mice (1.03 ± 0.50 ng/mL) compared to HC (0.46 ± 0.14, p < 0.001), HP (0.63 ± 0.28, p < 0.05) or CON mice (0.41 ± 0.14, p < 0.001). Thus, CR induces similar weight loss in aging mice irrespective of the diet’s macronutrient composition. An HF diet leads to excessive energy intake and pronounced gain in body fat in spite of increased fat oxidation and serum leptin during the refeeding after CR.
The Effects of 24-h Fasting on Exercise Performance and Metabolic Parameters in a Pilot Study of Female CrossFit Athletes
Authors: Eroglu Melike Nur , Rodríguez-Longobardo Celia , Ramírez-Adrados Ana , Colina-Coca Clara , Burgos-Postigo Silvia , López-Torres Olga , Fernández-Elías Valentín E.
Published: November 2023

Many studies have tested intermittent fasting (IF) in athletes, but its effects on female CrossFit athletes remain relatively unexplored in the existing literature. The aim of this study was to evaluate and compare the effects of 24-h IF on the physical performance of female CrossFit practitioners. Eleven female CrossFit athletes (age: 30.91 ± 3.42, weight: 65.26 ± 7.55 kg, height: 1.66 ± 0.05 m) participated in the study. The study used a crossover design with fasting and eating conditions. Participants completed an exercise test, standing long jump, and handgrip strength assessment. Hydration status, heart rate, blood lactate, blood glucose, rates of perceived exertion, and hunger were measured. Results showed significant differences in blood lactate concentration (F = 5.435, p = 0.042, η2p = 0.352). Resting blood lactate concentration was significantly lower in the fasting trial than in the eating trial (p < 0.001), but post-exercise blood lactate concentrations were higher in the fasting trial than in the eating trial (p < 0.001). No differences were found in performance times (p > 0.05). In conclusion, this pilot study of females suggests that 24-h fasting does not impair exercise performance or negatively affect physiological parameters in CrossFit athletes.
Vitamin D Attenuates Ulcerative Colitis by Inhibiting ACSL4-Mediated Ferroptosis
Authors: Gao Shuo , Sun Can , Kong Juan
Published: November 2023

Background: With environmental and lifestyle changes, recent epidemiological studies have shown that the prevalence of Ulcerative Colitis (UC) is on the rise, while treatment options are limited. There is an urgent need to explore the underlying mechanisms of vitamin D (VD) as an effective treatment. Methods: Dextran sulfate sodium-induced mice and lipopolysaccharide-induced HCT116 cells were used to establish the classic UC models in vivo and in vitro, respectively. Typical symbols of inflammation (IL-6, COX-2), oxidative stress (MDA, MPO, GSH), and ferroptosis (ACSL4, GPX4, SLC7A11, and Iron) were analyzed by Western blot, Immunohistochemistry, RT-PCR, and relative assay kits. The inflammation factors and oxidative stress injury of cells transfected with ACSL4+/+ plasmids were tested by Western blot, MDA, and MPO methods. Results: Vitamin D attenuated the levels of COX-2, IL-6, Iron, MDA, and MPO and improved SOD1 and GSH contents in DSS + VD and LPS + VD groups, compared with model groups. Ferrostatin-1 (Fer-1) could relieve the levels of COX-2, IL-6, Iron, MDA, and MPO while increasing the contents of SOD1 and GSH in DSS + Fer-1 and LPS + Fer-1 compared to model groups. VD downregulated the expression of ACSL4 and upregulated GPX4 in tissues and cells. After transfected with ACSL4+/+ plasmids, we found VD’s role of downregulating inflammation and oxidative stress was relieved. Conclusions: Vitamin D can relieve UC by inhibiting ferroptosis both in mice and in cells through the negative regulation of ACSL4, providing new insight into the therapeutic function of VD on UC.
The Role of Vitamin D in Patients with Inflammatory Bowel Disease Treated with Vedolizumab
Authors: Abraham Bincy P. , Fan Christopher , Thurston Theresa , Moskow Joshua , Malaty Hoda M.
Published: November 2023

Background: Many clinical factors can contribute to the efficacy of medical therapy in Inflammatory Bowel Disease (IBD). We assessed their effects on the efficacy of vedolizumab therapy in a cohort of patients with IBD. Methods: We conducted a retrospective study on patients between 18 and 80 years of age with ulcerative colitis (UC) or Crohn’s disease (CD) who were seen in the IBD program at Houston Methodist in Houston, TX and treated with vedolizumab for at least 6 months from 2018 to 2022. We investigated factors prior to the initiation of therapy that best predicted treatment response, with an emphasis on vitamin D levels and examined several variables including patients’ demographics and clinical information on disease location and severity and nutritional status before and after the initiation of vedolizumab. Post-treatment data were gathered after a minimum of 6 months of vedolizumab therapy. The clinical parameters used for the study were the Harvey–Bradshaw Index for CD and the Activity Index for UC. Results: There were 88 patients included in our study of whom 44 had CD and 44 had UC.; median age was 39.5 (31.0, 53.25) years; 34% patients were male; and 80.7% were Caucasian. All patients received an induction dosing of 300 mg vedolizumab at 0, 2, and 6 weeks then maintenance dosing as standard of care every 8 weeks. Among UC patients with vitamin D ≥ 30 ng/mL at the initiation of vedolizumab therapy, UC Endoscopic Index of Severity (UCEIS) scores after 6 months of therapy were significantly lower than in those who had low pre-treatment vitamin D levels (1.5 vs. 3.87, p = 0.037). After treatment, vitamin D levels improved more significantly in the higher pre-treatment vitamin D group, with a median level of 56 ng/mL, than in the lower pre-treatment vitamin D group, with a median level of only 31 ng/mL (p = 0.007). In patients with CD with vitamin D ≥ 30 ng/mL at the initiation of vedolizumab therapy, we found higher iron saturation (12 vs. 25%, p = 0.008) and higher vitamin B12 levels (433.5 vs. 885 pg/mL, p = 0.003) than in those with vitamin D < 30 ng/mL. After treatment, CD patients with high pre-treatment vitamin D levels had significantly higher vedolizumab levels (27.35 vs. 14.35 μg/mL, p = 0.045) than those with low pre-treatment vitamin D. Post-treatment scores and inflammatory markers in CD patients (HBI, CRP, ESR, and SES-CD) were lower in those who had lower baseline vitamin D. Conclusions: Our results show higher pre-treatment vitamin D levels predicted significant endoscopic improvement in patients with ulcerative colitis (UC). Improving vitamin D levels lowered C-reactive protein levels significantly in CD patients. Higher vitamin D levels were seen after treatment in both UC and CD patients. Vitamin D can play a role in clinical and endoscopic outcomes and should be assessed routinely and optimized in patients with IBD.
Development of a Semi-Quantitative Food-Frequency Questionnaire for Korean Adults with Obesity
Authors: Chung Jina , Ahn Seoeun , Joung Hyojee , Shin Sangah
Published: November 2023

The increasing prevalence of obesity is a serious concern in Korea. However, there is currently no available food-frequency questionnaire (FFQ) for examining the dietary patterns of adults with obesity. This study aimed to develop a semi-quantitative FFQ tailored to Korean adults with obesity. The dish/food items for the FFQ were extracted from the 24 h recall data of 8450 Korean adults (aged 19–64 years) with obesity who participated in the 2013–2019 Korea National Health and Nutrition Examination Survey. Among the 1709 dishes consumed, 475 were selected based on their high contribution to the intake or substantial between-individual variation in 11 nutrients: energy, carbohydrates, dietary fiber, sugar, fat, saturated fat, protein, sodium, vitamin A, vitamin E, and flavonoids. These dishes were subsequently categorized into 129 items based on their recipes and primary ingredients. The final 129 items included rice; noodles and dumplings; breads, rice cakes, and cereals; soups and stews; eggs, pulses, meat, and fish; vegetables and kimchi; fruit; snacks; beverages; milk/dairy products; alcohol; and water. The response options for intake frequency comprised nine options, and the intake amount response included three options (50%, 100%, and 150–200% of the standard intake). After validation, this FFQ is expected to be used in epidemiological studies to investigate the dietary patterns of Korean adults with obesity.
Dynamics and Crosstalk between Gut Microbiota, Metabolome, and Fecal Calprotectin in Very Preterm Infants: Insights into Feeding Intolerance
Authors: Hong Luyang , Huang Yihuang , Han Junyan , Li Shujuan , Zhang Lan , Jiang Siyuan , Zhou Qi , Cao Xincheng , Yu Weiyin , Yang Yi , Hong Shangyu , Zhou Yufeng , Yan Weili , Cao Yun
Published: November 2023

Background: Feeding intolerance (FI) is a significant concern in the care of preterm infants, impacting their growth and development. We previously reported that FI is linked to lower fecal calprotectin (FC) levels. This study aims to explore the postnatal dynamics and interplay between microbiota, metabolic profiles, and host immunity in preterm infants with and without FI. Methods: Infants with gestational age <32 weeks or birth weight <1500 g were enrolled at the Children’s Hospital of Fudan University between January 2018 and October 2020. Weekly fecal samples were analyzed for bacterial profiling, metabolome, and calprotectin levels, exploring their longitudinal development and interrelationships. Results: Of the 118 very preterm infants studied, 48 showed FI. These infants experienced an interrupted microbial–immune trajectory, particularly at 3–4 weeks of age, marked by a reduced bacterial abundance, alpha diversity, and FC levels. Metabolic changes in FI were pronounced between 3 and 6 weeks. Pantothenic acid and two polyamine metabolites were closely associated with bacterial abundance and FC levels and negatively correlated with the duration to attain full enteral feeding. Conclusions: FI infants demonstrated compromised microbiome–immune interactions, potentially influenced by specific metabolites. This research underscored the importance of early microbial and metabolic development in the pathogenesis of FI in very preterm infants.
Tick-Borne Co-Infections: Challenges in Molecular and Serologic Diagnoses
Authors: Sanchez-Vicente Santiago , Tokarz Rafal
Published: November 2023

Co-infections are a poorly understood aspect of tick-borne diseases. In the United States alone, nineteen different tick-borne pathogens have been identified. The majority of these agents are transmitted by only two tick species, Ixodes scapularis and Amblyomma americanum. Surveillance studies have demonstrated the presence of multiple pathogens in individual ticks suggesting a risk of polymicrobial transmission to humans. However, relatively few studies have explored this relationship and its impact on human disease. One of the key factors for this deficiency are the intrinsic limitations associated with molecular and serologic assays employed for the diagnosis of tick-borne diseases. Limitations in the sensitivity, specificity and most importantly, the capacity for inclusion of multiple agents within a single assay represent the primary challenges for the accurate detection of polymicrobial tick-borne infections. This review will focus on outlining these limitations and discuss potential solutions for the enhanced diagnosis of tick-borne co-infections.