bluuebunny/crossref_metadata_2025 huggingface dataset:
The captivating role of calcium in plant-microbe interaction
Authors: Bhar Anirban , Chakraborty Amrita , Roy Amit
Published: March 2023

Plant immune response is fascinating due to the complete absence of a humoral system. The adaptive immune response in plants relies on the intracellular orchestration of signalling molecules or intermediates associated with transcriptional reprogramming. Plant disease response phenomena largely depend on pathogen recognition, signal perception, and intracellular signal transduction. The pathogens possess specific pathogen-associated molecular patterns (PAMP) or microbe-associated molecular patterns (MAMP), which are first identified by pattern recognition receptors (PRRs) of host plants for successful infection. After successful pathogen recognition, the defence response is initiated within plants. The first line of non-specific defence response is called PAMP-triggered immunity (PTI), followed by the specific robust signalling is called effector-triggered immunity (ETI). Calcium plays a crucial role in both PTI and ETI. The biphasic induction of reactive oxygen species (ROS) is inevitable in any plant-microbe interaction. Calcium ions play crucial roles in the initial oxidative burst and ROS induction. Different pathogens can induce calcium accumulation in the cytosol ([Ca2+]Cyt), called calcium signatures. These calcium signatures further control the diverse defence-responsive proteins in the intracellular milieu. These calcium signatures then activate calcium-dependent protein kinases (CDPKs), calcium calmodulins (CaMs), calcineurin B-like proteins (CBLs), etc., to impart intricate defence signalling within the cell. Decoding this calcium ionic map is imperative to unveil any plant microbe interplay and modulate defence-responsive pathways. Hence, the present review is unique in developing concepts of calcium signature in plants and their subsequent decoding mechanism. This review also intends to articulate early sensing of calcium oscillation, signalling events, and comprehensive mechanistic roles of calcium within plants during pathogenic ingression. This will accumulate and summarize the exciting roles of calcium ions in plant immunity and provide the foundation for future research.
Nurses' knowledge, barriers and practice in the care of patients with delirium in the intensive care unit in Poland—A cross-sectional study
Authors: Lange Sandra , Mȩdrzycka-Da̧browska Wioletta , Tomaszek Lucyna , Wujtewicz Magdalena , Krupa Sabina
Published: March 2023

Background

Delirium is a cognitive disorder that occurs with high frequency in patients in intensive care units and affects patient outcomes. Despite recommendations for monitoring and assessing delirium in the ICU, studies show that it is still not routinely assessed and often remains undiagnosed or misinterpreted as dementia or depression.

Aim

The aim of this study was (1) to assess nurses' knowledge and clinical practices regarding delirium, (2) to identify the factors associated with nurses' knowledge, and (3) to define barriers to effective control of delirium.

Methods

A cross-sectional study was conducted among 371 ICU nurses in Poland.

Results

53.1% of nurses had never been educated on delirium control resulting in a deficit in knowledge of delirium symptoms, risk factors and complications associated with delirium in ICU patients. Master's degree in nursing (vs. Registered nurses + Bachelor's), female gender, and working in university hospital (vs. other) were positively correlated with nurse's knowledge, while age had a negative impact on knowledge. Delirium is a marginalized state in ICU patients, only 16.4% of nurses assessed delirium routinely and 35.8% assessed delirium occasionally, rarely using validated scales. Barriers to effective delirium control were primarily the lack of a requirement to assess delirium, the difficulty of assessing delirium in intubated patients and nurses' lack of confidence in their ability to use delirium assessment tools.

Conclusions

There is an urgent need to educate nurses about delirium and to make delirium assessment obligatory in clinical practice. The area of change should also include a hospital policy on delirium monitoring and management. The study was registered on ClinicalTrials.gov (NCT05384964).
Household polluting cooking fuels and adverse birth outcomes: An updated systematic review and meta-analysis
Authors: Luo Mengrui , Liu Tiancong , Ma Changcheng , Fang Jianwei , Zhao Zhiying , Wen Yu , Xia Yang , Zhao Yuhong , Ji Chao
Published: March 2023

Background and aim

The current study aimed to clarify the association between household polluting cooking fuels and adverse birth outcomes using previously published articles.

Methods

In this systematic review and meta-analysis, a systematic literature search in PubMed, Embase, Web of Science, and Scopus databases were undertaken for relevant studies that had been published from inception to 16 January 2023. We calculated the overall odds ratio (OR) and 95% confidence interval (CI) for adverse birth outcomes [low birth weight (LBW), small for gestational age (SGA), stillbirth, and preterm birth (PTB)] associated with polluting cooking fuels (biomass, coal, and kerosene). Subgroup analysis and meta-regression were also conducted.

Results

We included 16 cross-sectional, five case–control, and 11 cohort studies in the review. Polluting cooking fuels were found to be associated with LBW (OR: 1.37, 95% CI: 1.24, 1.52), SGA (OR: 1.48, 95% CI: 1.13, 1.94), stillbirth (OR: 1.38, 95% CI: 1.23, 1.55), and PTB (OR: 1.27, 95% CI: 1.19, 1.36). The results of most of the subgroup analyses were consistent with the main results. In the meta-regression of LBW, study design (cohort study: P < 0.01; cross-sectional study: P < 0.01) and sample size (≥ 1000: P < 0.01) were the covariates associated with heterogeneity. Cooking fuel types (mixed fuel: P < 0.05) were the potentially heterogeneous source in the SGA analysis.

Conclusion

The use of household polluting cooking fuels could be associated with LBW, SGA, stillbirth, and PTB. The limited literature, observational study design, exposure and outcome assessment, and residual confounding suggest that further strong epidemiological evidence with improved and standardized data was required to assess health risks from particular fuels and technologies utilized.
Disembodied AI and the limits to machine understanding of students' embodied interactions
Authors: Nathan Mitchell J.
Published: March 2023

The embodiment turn in the Learning Sciences has fueled growth of multimodal learning analytics to understand embodied interactions and make consequential educational decisions about students more rapidly, more accurately, and more personalized than ever before. Managing demands of complexity and speed is leading to growing reliance by education systems on disembodied artificial intelligence (dAI) programs, which, ironically, are inherently incapable of interpreting students' embodied interactions. This is fueling a potential crisis of complexity. Augmented intelligence systems offer promising avenues for managing this crisis by integrating the strengths of omnipresent dAI to detect complex patterns of student behavior from multimodal datastreams, with the strengths of humans to meaningfully interpret embodied interactions in service of consequential decision making to achieve a balance between complexity, interpretability, and accountability for allocating education resources to children.
Traffic lights detection and tracking for HD map creation
Authors: Mentasti Simone , Simsek Yusuf Can , Matteucci Matteo
Published: March 2023

HD-maps are one of the core components of the self-driving pipeline. Despite the effort of many companies to develop a completely independent vehicle, many state-of-the-art solutions rely on high-definition maps of the environment for localization and navigation. Nevertheless, the creation process of such maps can be complex and error-prone or expensive if performed via ad-hoc surveys. For this reason, robust automated solutions are required. One fundamental component of an high-definition map is traffic lights. In particular, traffic light detection has been a well-known problem in the autonomous driving field. Still, the focus has always been on the light state, not the features (i.e., shape, orientation, pictogram). This work presents a pipeline for lights HD-map creation designed to provide accurate georeferenced position and description of all traffic lights seen by a camera mounted on a surveying vehicle. Our algorithm considers consecutive detection of the same light and uses Kalman filtering techniques to provide each target’s smoother and more precise position. Our pipeline has been validated for the detection and mapping task using the state-of-the-art dataset DriveU Traffic Light Dataset. The results show that our model is robust even with noisy GPS data. Moreover, for the detection task, we highlight how our model can correctly identify even far-away targets which are not labeled in the original dataset.
Risky sexual behaviors and their associated factors within high school students from Collège Saint André in Kigali, Rwanda: An institution-based cross-sectional study
Authors: Ndagijimana Emmanuel , Biracyaza Emmanuel , Nzayirambaho Manasse
Published: March 2023

Background

Risky sexual behaviors (RSBs) remain public health concerns in adolescents from sub-Saharan Africa (SSA), and these practices may increase vulnerability to reproductive health problems if no early healthcare strategies are implemented. While previous studies reported that adolescents are engaged in these RSBs due to diverse influences such as the teenage stage, urbanization, and change in the environment they experience, there is a shortage of studies on RSB among adolescents in SSA. This study assessed the magnitude of RSBs and the RSB-associated factors among in-school adolescents.

Methods

School-based cross-sectional study was conducted among 263 Saint Andre school students in Kigali, Rwanda, from July 3, 2020, to September 30, 2020. Systematic random sampling techniques were employed. All data were entered into Epi-Data and analyzed using SPSS version 25. Chi-square tests and multivariable logistic regression analyses were applied to determine factors associated with risky sexual behaviors. Confidence intervals (CIs) of 95% and 5% for statistical significance were maintained.

Results

Of 263 participants, 109 (41%) experienced RSB in their lifetime. Among them, 66 respondents (60.55%) utilized contraceptive methods to prevent sexual and reproductive problems that can be caused by unprotected sexual intercourse. The students who experienced domestic violence had increased odds of experiencing RSB [odds ratio (OR) = 4.22; 95% CI: 1.6–11.23] than their counterparts. Those in grade 11 (OR = 2.68; 95% CI: 1.06–6.78) and grade 12 (OR = 4.39; 95% CI: 1.82–10.56) were more likely to practice RSB than those in grade 10. Alcohol users were almost more likely to experience RSB (OR = 3.9; 95% CI: 1.97–5.5) than their counterparts. Those who lived away from their biological parents had higher likelihood of experiencing RSB (OR = 2.5; 95% CI: 1.14–4.42) than those who lived with one or both parents. Students who experienced peer pressure were more likely to engage in RSB (OR = 3.9; 95% CI: 2.01–7.51) than their counterparts.

Conclusion

Promoting specific intervention programs built upon the factors associated with RSB among high school students needs to be prioritized.
Reduction of cybersickness in head mounted displays use: A systematic review and taxonomy of current strategies
Authors: Ang Samuel , Quarles John
Published: March 2023

This literature review examines the existing research into cybersickness reduction with regards to head mounted display use. Cybersickness refers to a collection of negative symptoms sometimes experienced as the result of being immersed in a virtual environment, such as nausea, dizziness, or eye strain. These symptoms can prevent individuals from utilizing virtual reality (VR) technologies, so discovering new methods of reducing them is critical. Our objective in this literature review is to provide a better picture of what cybersickness reduction techniques exist, the quantity of research demonstrating their effectiveness, and the virtual scenes testing has taken place in. This will help to direct researches towards promising avenues, and illuminate gaps in the literature. Following the preferred reporting items for systematic reviews and meta-analyses statement, we obtained a batch of 1,055 papers through the use of software aids. We selected 88 papers that examine potential cybersickness reduction approaches. Our acceptance criteria required that papers examined malleable conditions that could be conceivably modified for everyday use, examined techniques in conjunction with head mounted displays, and compared cybersickness levels between two or more user conditions. These papers were sorted into categories based on their general approach to combating cybersickness, and labeled based on the presence of statistically significant results, the use of virtual vehicles, the level of visual realism, and the virtual scene contents used in evaluation of their effectiveness. In doing this we have created a snapshot of the literature to date so that researchers may better understand what approaches are being researched, and the types of virtual experiences used in their evaluation. Keywords: Virtual reality cybersickness Simulator Sickness Visually induced motion sickness reduction Systematic review Head mounted display.
Exclusion by design: The undocumented 1.5 generation in the U.S
Authors: Sanchez Linda E.
Published: March 2023

This article focuses on Mexican individuals who grew up in the U.S. (1.5 generation) without documents and were not able to benefit from Deferred Action for Childhood Arrivals (DACA) or who were unable to renew their DACA. A 2012 Executive Action by former president Obama, DACA gave some undocumented youth relief from deportation and a 2-year renewable work permit provided they met certain criteria. Undocumented individuals DACA failed to reach have generally been overlooked in immigration research in favor of examining how DACA recipients' lives have been transformed by DACA. This project helps fill this gap by examining life outside of DACA, and how the program acted as an internal U.S. border of exclusion for many. This research also aids in understanding the impacts of changing government policies on vulnerable populations, especially those who are in some respects made even more vulnerable by their faith in the government, fear of the government, or are actively excluded from government programs. This investigation is part of a study that compares 20 DACA beneficiaries to 20 individuals without DACA. Through ethnographic methodologies and one-on-one interviews, this article examines the 20 research participants who fall outside DACA. It investigates why people who qualified for DACA did not apply, barriers to applying/renewing, and how members of the 1.5 generation were excluded from the program by restrictions such as date of arrival requirements. The article discusses what it means for research participants to live outside of DACA, and how they see their lives because they do not have DACA while others do. For example, what does it mean to age out of qualifying for DACA? What actions did individuals then take regarding their lack of legal status?
Application of Clavien–Dindo classfication-grade in evaluating overall efficacy of laparoscopic pancreaticoduodenectomy
Authors: Song Xiangyang , Ma Yu , Shi Hongyun , Liu Yahui
Published: March 2023

Background

The Clavien–Dindo classification (CDC) has been widely accepted and applied in clinical practice. We investigated its effectiveness in prediction of major complications (LPPC) after laparoscopic pancreaticoduodenectomy (LPD) and associated risk factors.

Methods

A retrospective analysis was conducted covering clinical data of 793 patients undergoing LPD from April 2015 to November 2021. CDC was utilized to grade postoperative complications and analyze the differences. Risk factors of LPPC were identified according to univariate and multivariate analyses.

Resluts

For the 793 patients undergoing laparoscopic pancreaticoduodenectomy in the northeast of China, LPPC was reported in 260 (32.8%) patients, pancreatic fistula in 169 (21.3%), biliary fistula in 44 (5.5%), delayed gastric emptying in 17(2.1%), post pancreatectomy hemorrhage in 55 (6.9%), intestinal fistula in 7 (0.8%), abdominal infections in 59 (7.4%) and pulmonary complication in 28 (3.5%). All complications were classified into five levels with the C–D classification (Grade I–V), with 83 (31.9%) patients as grade I, 91 (35.0%) as grade II, 38 (14.6%) as grade IIIa, 24 (9.2%) as grade IIIb, 9 (3.5%) as grade IV and 15 (5.8%) as grade V. 86 (10.8%) patients experienced major complications (grade III–V).The results of univariate and multivariate analysis revealed the independent risk factors for laparoscopic pancreaticoduodenectomy complications to be preoperative total bilirubin (P = 0.029, OR = 1.523), soft pancreas texture (P < 0.001, OR = 1.399), male (P = 0.038, OR = 1.396) and intraoperative transfusion (P = 0.033, OR = 1.517). Preoperative total bilirubin (P = 0.036, OR = 1.906) and intraoperative transfusions (P = 0.004, OR = 2.123) were independently associated with major postoperative complications. The influence of different bilirubin levels on C–D grade of complications was statistically significant (P = 0.036, OR = 1.906).

Conclusions

The Clavien–Dindo classification (CDC) may serve as a valid tool to predict major postoperative complications and contribute to perioperative management and comparison of surgical techniques in different medical centers.
Effect of N6-methyladenosine methylation-related gene signature for predicting the prognosis of hepatocellular carcinoma patients
Authors: Zheng Xinyu , Zhang Yingyue , Wang Yun , He Zijing , Zhang Qiang , Ren Dapeng , Yan Xiao , Yuan Xiao
Published: March 2023

Background and aims

Hepatocellular carcinoma (HCC) is a common cause of cancer-related death in humans. Increasing evidence indicates that an imbalance in N6-methyladenosine (m6A) methylation is linked to the occurrence and development of cancer. We then developed a prognostic model as an independent risk factor with which predict the prognosis of HCC.

Methods

We obtained the gene expression and clinical data of HCC patients from the TCGA databases. The prognostic value of m6A methylation-related genes in patients who had HCC were subjected to comprehensive bioinformatics analysis. We use RiskScore=∑i=1nCoefi×Xi to construct the risk scoring formula. We collected pathological specimens from 68 patients who had HCC, and conducted immunohistochemical staining experiments on the specimens.

Results

There was a significant correlation between candidate m6A methylation-related genes (YTHDF2, METTL14 and ZC3H13) overall survival of HCC patients. Among the 68 HCC patient specimens that underwent immunohistochemical staining, all cancer tissues were positive for METTL14, YTHDF2, and ZC3H13 staining in contrast to the adjacent tissues. We conducted a Kaplan-Meier survival analysis. The results showed that patients who had low METTL14 expression had a longer survival time than those of patients who had high METTL14 expression. Also, patients with low YTHDF2 expression had a longer survival time than patients with high YTHDF2 expression. Finally, patients with high ZC3H13 expression lived longer than those with low ZC3H13 expression. This result is consistent with the bioinformatics analysis conclusion above.

Conclusions

Generally, the prognostic model that was based on m6A methylation-related genes in this study can effectively predict the prognosis of HCC patients.
Age-Related changes in the morphological features of medial column of the proximal humerus in the Chinese population
Authors: Chang Zuhao , Zhu Zhengguo , Zhang Wei , Chen Hua , Liu Yujie , Tang Peifu
Published: March 2023

Background

Age-related changes in the medial column (MC) of the proximal humerus have a major impact on fracture management; however, the changes in the morphological features remain unclear. This study aimed to investigate the age-related changes in the morphological features of MC and present the morphological grading.

Methods

One hundred computed tomography (CT) images of the proximal humerus of 100 individuals (19–95 years) were retrospectively obtained. The individuals were categorized into five age groups to quantify the differences among different ages; the youngest group (18–44 years) served as the baseline group. Parameters of the morphological features were measured on CT images with multiplanar reconstruction based on an explicit definition of MC, including length, thickness, width, oblique thickness (DSM), humeral head diameter (DHM), and ratio (RSM) of DSM to DHM. The morphological grading of MC was presented based on the value of RSM deviating different standard deviations (SD) from the mean value in the baseline group.

Results

Significant negative correlations were observed between age and the morphological parameters of MC (r ranged from −0.875 to −0.926; all P < 0.05), excluding DHM (r = 0.081, P = 0.422). Significant differences in the values of morphological feature parameters were detected among the five age groups (all P < 0.001). The highest mean values of morphological feature parameters were observed in the youngest group (18–44 years), which decreased gradually with increasing age until the lowest mean values were observed in the oldest group (≥90 years) (all P < 0.05). The morphological features of MC were categorized into three grades based on the value of RSM deviating 1.5 SD or 3 SD from the mean value in the baseline group.

Conclusion

Our study shows that the parameter values of morphological features of MC decreased with increasing age. The morphological features of MC could be categorized into three grades. Our findings may provide a more comprehensive insight into age-related changes in the morphological features of MC that facilitate risk stratification and optimize the management of proximal humeral fractures.
Validation of a novel 3D printed positioning device and dynamic radiographic technique to quantify rotational laxity of the stifle in dogs
Authors: Yu Jin , Griffon Dominique J. , Wisser Gary , Mostafa Ayman A. , Dong Fanglong
Published: March 2023

Cranial cruciate ligament deficiency (CCLD) results in internal rotational instability of the stifle (RLS). By contrast, tibial torsion (TT) is an anatomical feature of the tibia along its longitudinal axis. The objective of this study was to validate a dynamic radiographic technique to measure internal rotational laxity of the stifle and differentiate it from TT. Models included transection of the CCL for RLS and an osteotomy for TT. One limb within eight pairs of canine cadaveric hind limbs was randomly assigned to CCLD. The contralateral limb underwent TT, followed by CCLD. Neutral and stress radiographs were taken with the limb in a custom rotating 3-D printed positioning device before and after each modification. The position of the calcaneus on neutral views and the magnitude of its displacement under standardized torque were compared within limbs and between groups. Transection of the CCL increased the magnitude of displacement of the calcaneus by 1.6 mm (0.3–3.1 mm,p< 0.05) within limbs. The lateral calcaneal displacement (dS-dN) tended to be greater when CCLD limbs were compared to limbs with intact CCL. A magnitude of calcaneal displacement of 3.45 mm differentiated limbs with RLS from intact limbs with 87.5% sensitivity and 68.7% specificity. The calcaneus was displaced further laterally by about 3 mm on neutral radiographs (dN) when limbs with experimental TT were compared to those without TT (p< 0.05). A calcaneus located at least 3.25 mm from the sulcus differentiated limbs with TT from intact limbs with 87.5% sensitivity and 87.5% specificity. The technique reported here allowed detection of RLS, especially within limbs. A calcaneus located at least 3.25 mm on neutral radiographs of large dogs should prompt a presumptive diagnosis of TT.
Association Between Multiple Chronic Conditions and Depressive Symptoms Among Older Adults in China: Evidence From the China Health and Retirement Longitudinal Study (CHARLS)
Authors: Liu Haixia , Zhou Zhongliang , Fan Xiaojing , Shen Chi , Ma Yang , Sun Hongwei , Xu Zhaoyang
Published: March 2023

Objectives: The purpose of this study was to explore the association between multiple chronic conditions (MCCs) and depressive symptoms among older adults in China.

Methods: We used the fourth round of data from the China Health and Retirement Longitudinal Study, and included 9789 older adults aged 60 years old and above.

Results: Compared with the older adults without chronic disease, older adults with MCCs and those with one chronic disease had a high risk of depression (MCCs: OR-adjusted: 1.55, 95% CI: 1.37 to 1.75; one chronic disease: OR-adjusted: 1.20, 95% CI: 1.05–1.37). In urban areas, the prevalence among older adults with MCCs was 2.01 times higher than that among older adults without chronic disease (OR-adjusted: 2.01, 95% CI: 1.56–2.60), while it was 1.44 times higher in rural areas (OR-adjusted:1.44, 95% CI: 1.25–1.65). Self-reported health, duration of sleep, social activities, and type of medical insurance were the influencing factors of depressive symptoms in older adults.

Conclusion: This study contributed to enriching the research on the relationship between MCCs and depressive symptoms in older adults in China.
Storytelling with Image Data: A Systematic Review and Comparative Analysis of Methods and Tools
Authors: Lotfi Fariba , Beheshti Amin , Farhood Helia , Pooshideh Matineh , Jamzad Mansour , Beigy Hamid
Published: March 2023

In our digital age, data are generated constantly from public and private sources, social media platforms, and the Internet of Things. A significant portion of this information comes in the form of unstructured images and videos, such as the 95 million daily photos and videos shared on Instagram and the 136 billion images available on Google Images. Despite advances in image processing and analytics, the current state of the art lacks effective methods for discovering, linking, and comprehending image data. Consider, for instance, the images from a crime scene that hold critical information for a police investigation. Currently, no system can interactively generate a comprehensive narrative of events from the incident to the conclusion of the investigation. To address this gap in research, we have conducted a thorough systematic literature review of existing methods, from labeling and captioning to extraction, enrichment, and transforming image data into contextualized information and knowledge. Our review has led us to propose the vision of storytelling with image data, an innovative framework designed to address fundamental challenges in image data comprehension. In particular, we focus on the research problem of understanding image data in general and, specifically, curating, summarizing, linking, and presenting large amounts of image data in a digestible manner to users. In this context, storytelling serves as an appropriate metaphor, as it can capture and depict the narratives and insights locked within the relationships among data stored across different islands. Additionally, a story can be subjective and told from various perspectives, ranging from a highly abstract narrative to a highly detailed one.
Line-Level Layout Recognition of Historical Documents with Background Knowledge
Authors: Fischer Norbert , Hartelt Alexander , Puppe Frank
Published: March 2023

Digitization and transcription of historic documents offer new research opportunities for humanists and are the topics of many edition projects. However, manual work is still required for the main phases of layout recognition and the subsequent optical character recognition (OCR) of early printed documents. This paper describes and evaluates how deep learning approaches recognize text lines and can be extended to layout recognition using background knowledge. The evaluation was performed on five corpora of early prints from the 15th and 16th Centuries, representing a variety of layout features. While the main text with standard layouts could be recognized in the correct reading order with a precision and recall of up to 99.9%, also complex layouts were recognized at a rate as high as 90% by using background knowledge, the full potential of which was revealed if many pages of the same source were transcribed.
Effects of Weather on Sugarcane Aphid Infestation and Movement in Oklahoma
Authors: Lee Seokil , Vitale Jeffrey , Lambert Dayton , Vitale Pilja , Elliot Norman , Giles Kristopher
Published: March 2023

Sugarcane aphids have caused economic damage on sorghum and other grain production in Oklahoma. When applied in a timely manner, insecticides provide efficient control; however, it is difficult to protect against the unexpected heavy infestations that have appeared frequently since 2016. This article evaluates the effect of spatial and temporal patterns of weather variables on sugarcane infestation airborne movements. Econometric methods identified persistent northwesterly wind patterns that explain aphid movements. Results serve as a base for sugarcane aphid infestation predictions and to assist stakeholders in developing an early warning system for sorghum producers.
Application of Edible Coating in Extension of Fruit Shelf Life: Review
Authors: Pham Thanh Tung , Nguyen Lien Le Phuong , Dam Mai Sao , Baranyai Laszlo
Published: March 2023

In the past few decades, fruits have been increasingly consumed, leading to an increase in global fruit production. However, fresh produce is susceptible to large losses during production and preservation. In the postharvest preservation stage, fruits undergo various technical treatments for maintaining their quality. A widely adopted technology is the application of edible coatings, which can be applied to a diverse range of fruits to regulate the exchange of moisture and gases between the fruit and its environment. In addition, edible coatings provide a significant benefit by allowing the integration of different active ingredients into the coating’s matrix, meaning that these substances will associate with and possibly be eaten together with the fruit. This would help improve the organoleptic and nutritional qualities of the fruit as well as the shelf life. This paper provides an overview of the available data on the typical components used in coating matrix, focusing on the effect of the material combinations and application techniques to fruit properties. The processors can use this knowledge in choosing a suitable coating material and concentration for various fresh and fresh-cut fruits. Additionally, this paper reviews recent developments and limitations in utilizing edible coatings for prolonging the shelf-life of fruits.
Spanish Fuet Sausages Fat-Reduced to Diminish Boar Taint: Sensory and Technological Quality
Authors: Peñaranda Irene , Egea Macarena , Álvarez Daniel , Garrido María Dolores , Linares María Belén
Published: March 2023

Reduced-fat cured sausages were evaluated as a strategy to reduce boar taint in entire male pork products with high levels of androstenone and skatole, both lipophilic. Three fuet-type sausages (two replicates each) were developed: the control (C) (60% lean, 33.69% fat), and two reduced-fat (60% lean 21.19% fat) products; R1, 6% inulin, 0.5% β-glucan and R2, 3% inulin, 0.5% grape skin and 1% β-glucan. All of them were prepared from entire male pork with an androstenone concentration of 6.887 µg/g and 0.520 µg/g of skatole. Significant differences (p ≤ 0.001) in the moisture content were described between the fuet R1 and the C and R2, which obtained the highest percentage. Regarding the CIELAB, the C samples had the highest L* value, while the R2 sausages were the darkest. Boar taint was reduced in both R1 and R2, with a greater reduction in R2 (p ≤ 0.000). The addition of inulin and β-glucan in fuet R1 provided a similar technological and sensory profile to C. However, both strategies provided a reduction of sexual odour, which was higher when grape skins were included. In addition, R2 gave a characteristic sausage with more odour and flavour, dark colour and overall rating than C and R1.
The Gut Microbiota of Young Asian Elephants with Different Milk-Containing Diets
Authors: Zhang Chengbo , Chen Junmin , Wu Qian , Xu Bo , Huang Zunxi
Published: March 2023

Evaluating the association between milk-containing diets and the microbiomes of young Asian elephants could assist establishing optimal breast milk supplementation to improve offspring survival rates. The microbiomes of young Asian elephants on different milk-containing diets (elephant milk only, elephant milk–plant mixed feed, and goat milk–plant mixed feed) were investigated using high-throughput sequencing of 16S rRNA genes and phylogenetic analysis. Microbial diversity was lower in the elephant milk-only diet group, with a high abundance of Proteobacteria compared to the mixed-feed diet groups. Firmicutes and Bacteroidetes were dominant in all groups. Spirochaetae, Lachnospiraceae, and Rikenellaceae were abundant in the elephant milk–plant mixed-feed diet group, and Prevotellaceae was abundant in the goat milk–plant mixed-feed diet group. Membrane transport and cell motility metabolic pathways were significantly enriched in the elephant milk–plant mixed-feed diet group, whereas amino acid metabolism and signal transduction pathways were significantly enriched in the goat milk–plant mixed-feed diet group. The intestinal microbial community composition and associated functions varied significantly between diets. The results suggest that goat milk is not suitable for young elephants. Furthermore, we provide new research methods and directions regarding milk source evaluation to improve elephant survival, wellbeing, and conservation.
Cutaneous Lymphoma and Antibody-Directed Therapies
Authors: Sernicola Alvise , Ciolfi Christian , Miceli Paola , Alaibac Mauro
Published: March 2023

The introduction of monoclonal antibodies such as rituximab to the treatment of cancer has greatly advanced the treatment scenario in onco-hematology. However, the response to these agents may be limited by insufficient efficacy or resistance. Antibody–drug conjugates are an attractive strategy to deliver payloads of toxicity or radiation with high selectivity toward malignant targets and limited unwanted effects. Primary cutaneous lymphomas are a heterogeneous group of disorders and a current area of unmet need in dermato-oncology due to the limited options available for advanced cases. This review briefly summarizes our current understanding of T and B cell lymphomagenesis, with a focus on recognized molecular alterations that may provide investigative therapeutic targets. The authors reviewed antibody-directed therapies investigated in the setting of lymphoma: this term includes a broad spectrum of approaches, from antibody–drug conjugates such as brentuximab vedotin, to bi-specific antibodies, antibody combinations, antibody-conjugated nanotherapeutics, radioimmunotherapy and, finally, photoimmunotherapy with specific antibody–photoadsorber conjugates, as an attractive strategy in development for the future management of cutaneous lymphoma.
Inhibition of Erythromycin and Erythromycin-Induced Resistance among Staphylococcus aureus Clinical Isolates
Authors: Mahfouz Aya A. , Said Heba S. , Elfeky Sherin M. , Shaaban Mona I.
Published: March 2023

The increasing incidence of erythromycin and erythromycin-induced resistance to clindamycin among Staphylococcus aureus (S. aureus) is a serious problem. Patients infected with inducible resistance phenotypes may fail to respond to clindamycin. This study aimed to identify the prevalence of erythromycin and erythromycin-induced resistance and assess for potential inhibitors. A total of 99 isolates were purified from various clinical sources. Phenotypic detection of macrolide-lincosamide-streptogramin B (MLSB)-resistance phenotypes was performed by D-test. MLSB-resistance genes were identified using PCR. Different compounds were tested for their effects on erythromycin and inducible clindamycin resistance by broth microdilution and checkerboard microdilution methods. The obtained data were evaluated using docking analysis. Ninety-one isolates were S. aureus. The prevalence of constitutive MLSB, inducible MLSB, and macrolide-streptogramin (MS) phenotypes was 39.6%, 14.3%, and 2.2%, respectively. Genes including ermC, ermA, ermB, msrA, msrB, lnuA, and mphC were found in 82.6%, 5.8%, 7.7%, 3.8%, 3.8%, 13.5%, and 3.8% of isolates, respectively. Erythromycin resistance was significantly reduced by doxorubicin, neomycin, and omeprazole. Quinine, ketoprofen, and fosfomycin combated and reversed erythromycin/clindamycin-induced resistance. This study highlighted the significance of managing antibiotic resistance and overcoming clindamycin treatment failure. Doxorubicin, neomycin, omeprazole, quinine, ketoprofen, and fosfomycin could be potential inhibitors of erythromycin and inducible clindamycin resistance.
Sedeveria pink ruby Extract-Mediated Synthesis of Gold and Silver Nanoparticles and Their Bioactivity against Livestock Pathogens and in Different Cell Lines
Authors: Kuppusamy Palaniselvam , Kim Sujung , Kim Sung-Jo , Park Myunghum , Song Ki-Duk
Published: March 2023

Biological synthesis of metal nanoparticles has a significant impact in developing sustainable technologies for human, animal, and environmental safety. In this study, we synthesized gold and silver nanoparticles (NPs) using Sedeveria pink ruby (SP) extract and characterized them using UV–visible spectrophotometry, FESEM-EDX, HR-TEM, XRD, and FT-IR spectroscopy. Furthermore, antimicrobial and antioxidant activities and cytotoxicity of the synthesized NPs were evaluated. UV–visible absorption spectra showed λmax at 531 and 410 nm, corresponding to the presence of SP gold NPs (SP-AuNPs) and SP silver NPs (SP-AgNPs). Most NPs were spherical and a few were triangular rods, measuring 5–30 and 10–40 nm, respectively. EDX elemental composition analysis revealed that SP-AuNPs and SP-AgNPs accounted for >60% and 30% of NPs, respectively. Additionally, some organic moieties were present, likely derived from various metabolites in the natural plant extract, which acted as stabilizing and reducing agents. Next, the antimicrobial activity of the NPs against pathogenic microbes was tested. SP-AgNPs showed potent antibacterial activity against Escherichia coli and Yersinia pseudotuberculosis. Moreover, at moderate and low concentrations, both NPs exhibited weak cytotoxicity in chicken fibroblasts (DF-1) and macrophages (HD11) as well as human intestinal cancer cells (HT-29). Meanwhile, at high concentrations, the NPs exhibited strong cytotoxicity in both chicken and human cell lines. Therefore, the synthesized SP-AuNPs and SP-AgNPs may act as promising materials to treat poultry diseases.
Transcriptome Analysis of Cyclooctasulfur Oxidation and Reduction by the Neutrophilic Chemolithoautotrophic Sulfurovum indicum from Deep-Sea Hydrothermal Ecosystems
Authors: Wang Shasha , Jiang Lijing , Cui Liang , Alain Karine , Xie Shaobin , Shao Zongze
Published: March 2023

Chemolithoautotrophic Campylobacterota are widespread and predominant in worldwide hydrothermal vents, and they are key players in the turnover of zero-valence sulfur. However, at present, the mechanism of cyclooctasulfur activation and catabolism in Campylobacterota bacteria is not clearly understood. Here, we investigated these processes in a hydrothermal vent isolate named Sulfurovum indicum ST-419. A transcriptome analysis revealed that multiple genes related to biofilm formation were highly expressed during both sulfur oxidation and reduction. Additionally, biofilms containing cells and EPS coated on sulfur particles were observed by SEM, suggesting that biofilm formation may be involved in S0 activation in Sulfurovum species. Meanwhile, several genes encoding the outer membrane proteins of OprD family were also highly expressed, and among them, gene IMZ28_RS00565 exhibited significantly high expressions by 2.53- and 7.63-fold changes under both conditions, respectively, which may play a role in sulfur uptake. However, other mechanisms could be involved in sulfur activation and uptake, as experiments with dialysis bags showed that direct contact between cells and sulfur particles was not mandatory for sulfur reduction activity, whereas cell growth via sulfur oxidation did require direct contact. This indirect reaction could be ascribed to the role of H2S and/or other thiol-containing compounds, such as cysteine and GSH, which could be produced in the culture medium during sulfur reduction. In the periplasm, the sulfur-oxidation-multienzyme complexes soxABXY1Z1 and soxCDY2Z2 are likely responsible for thiosulfate oxidation and S0 oxidation, respectively. In addition, among the four psr gene clusters encoding polysulfide reductases, only psrA3B3C3 was significantly upregulated under the sulfur reduction condition, implying its essential role in sulfur reduction. These results expand our understanding of the interactions of Campylobacterota with the zero-valence sulfur and their adaptability to deep-sea hydrothermal environments.
Antioxidant and Anti-Tumor Effects of Dietary Vitamins A, C, and E
Authors: Didier Alexander J. , Stiene Jennifer , Fang Lauren , Watkins Dean , Dworkin Lance D. , Creeden Justin F.
Published: March 2023

Oxidative stress, a condition characterized by an imbalance between pro-oxidant molecules and antioxidant defense systems, is increasingly recognized as a key contributor to cancer development. This is because the reactive oxygen species (ROS) generated during oxidative stress can damage DNA, proteins, and lipids to facilitate mutations and other cellular changes that promote cancer growth. Antioxidant supplementation is a potential strategy for decreasing cancer incidence; by reducing oxidative stress, DNA damage and other deleterious cellular changes may be attenuated. Several clinical trials have been conducted to investigate the role of antioxidant supplements in cancer prevention. Some studies have found that antioxidant supplements, such as vitamin A, vitamin C, and vitamin E, can reduce the risk of certain types of cancer. On the other hand, some studies posit an increased risk of cancer with antioxidant supplement use. In this review, we will provide an overview of the current understanding of the role of oxidative stress in cancer formation, as well as the potential benefits of antioxidant supplementation in cancer prevention. Additionally, we will discuss both preclinical and clinical studies highlighting the potentials and limitations of preventive antioxidant strategies.
Improvement Technique for Group Search Optimization Using Experimental Design Method
Authors: Yang Po-Yuan , Yang Kai-Yu , Ho Wen-Hsien , Chou Fu-I , Chou Jyh-Horng
Published: March 2023

This study proposes the use of an experimental design approach in GSO, and a systematic approach to deal with the hyperparameter settings of GSOs and to provide stable algorithmic performance of GSOs through the experimental design approach. To address these two issues, this study explores the combination of hyperparameters that can improve the performance of GSOs using a uniform design. In addition, the Taguchi method and optimal operations were used to derive an excellent combination of parameters that would provide the best value and robustness of the function to provide a stable performance of GSO. The validity of the performance of the proposed method was tested using ten benchmark functions, including three unimodal, three multimodal, and four restricted multimodal functions. The results were compared with the t-distribution test in addition to the mean and standard deviation to analyze their validity. The results of the t-distribution test showed that the p-values obtained for both UD-GSO and R-GSO were less than 0.05, indicating significant differences compared with GSO for both unimodal and multimodal functions. Two restricted multimodal functions are not significantly different, while the other two are below 0.05, indicating significant differences. This shows that the performance obtained using UD-GSO and R-GSO is more effective than the original GSO. UD-GSO and R-GSO provide better and more robust results than GSO. The main contributions of this paper are as follows: (i) This study proposes a uniform design approach to overcome the difficulties of setting hyperparameters in GSO. (ii) This study proposes a Taguchi method and optimal operation to provide a robust calculation for GSO. (iii) The method applied in this study provides systematic parameter design to solve GSO parameter setting and robust result obtaining.
Discrete Wavelet Transform—Based Metal Material Analysis Model by Constant Phase Angle Pulse Eddy Current Method
Authors: Xie Yong , Yu Yating , Li Liangting
Published: March 2023

Traditional eddy current technology identifies metal information with information of single frequency of limited frequency spectrum. To solve existing problems, this paper proposes a discrete wavelet transform-based metal material analysis model by using a constant phase angle pulse eddy current (CPA-PEC) sensor which collects and depicts metal feature information from multiple dimensions; then, the quantification calculation model of metal material by CPA-PEC feature is presented; finally, an experimental platform is built to collect the CPA-PEC features of various metal samples and verify recognition accuracy of the proposed metal material analysis model. In the investigation, 1000 eddy current signals from four standard metals (Cu, Fe, Al, St) and three types of metallic irons (Fe-K162, Fe-K163, Fe-K240) are measured and the features are identified by discrete wavelet transform. The feature correlation and significance are determined by regression analysis. Finally, the calculation model of feature evaluation index is present. The experimental analysis indicates that the stability of the quantitative evaluation index of eddy current features reaches 97.1%, the comprehensive accuracy error is less than 0.32% and the average measurement speed is about 50 ms for 1000 random sampling tests on standard metals.
Application of the Falling Number Method in the Evaluation of the α-Amylase Activity of Malt Flour
Authors: Jukić Marko , Šumanovac Franjo , Nakov Gjore , Šimić Gordana , Komlenić Daliborka Koceva , Ivanova Nastia , Lukinac Jasmina
Published: March 2023

The Falling Number (FN) test is not suitable for the determination of α-amylase activity in malt flour because the amylolytic activity is too high to be determined by the FN instrument. The aim of this study was to modify the FN method to allow quantification of high α-amylase activity in malt flour. The modifications were performed in two ways: using different amounts of malt flour (0.05, 0.1, 0.2, and 0.3 g) or by using malt extracts (0.5, 1, 2, 3, 4, 5, and 6 mL). In both cases, 6 g of standard wheat starch was used as substrate. The results of the modified methods were compared with the α-amylase activity determined by the standard Ceralpha method. Linear and nonlinear exponential regression was used to calculate the predicted amylase activity, and Bland-Altman analysis was used to measure the agreement between standard and modified methods. The modifications of the FN method using 0.1 g of malt flour showed that the modified method was able to accurately measure high levels of α-amylase activity in malt flour, and the results obtained by the modified method were in agreement with the results obtained by the standard Ceralpha method.