bluuebunny/crossref_metadata_2025 huggingface dataset:
Gait Parameters of Elite Kickboxing Athletes
Authors: Niewczas Marta , Rydzik Łukasz , Ambroży Tadeusz , Wąsacz Wojciech , Spieszny Michał , Perliński Jacek , Javdaneh Norollah
Published: September 2023

Background: Correct movement techniques are crucial for enhancing athletic performance and minimizing the risk of injury. This is particularly true for kickboxing, in which a variety of forms of locomotion are employed. Despite its importance, gait and movement analysis in this sport is under-researched. The primary objective of this study is to understand gait patterns and limb symmetry between the dominant and non-dominant legs among professional kickboxers and to explore their correlation with the level of technical and tactical preparation. Methods: A cross-sectional observational study was conducted involving 20 elite kickboxers. Data collection employed the 3D Force Treadmill for gait parameters, as well as simulated sparring sessions to evaluate technical–tactical indices. Various gait parameters were analyzed, including the center of pressure (COP), gait phase, spatial and time parameters, as well as reaction force. Results: No significant bilateral differences were found in the majority of gait variables. Exceptions were in the medio-lateral component of the COP and force, where more deviation was observed in the non-dominant foot. The only noted significant correlation was between lateral symmetry and attack activity. Conclusions: This study allows us to indicate that kickboxers’ training promotes symmetrical gait patterns. Targeted training interventions could further optimize these patterns. The significant relationship between lateral symmetry and attack activity suggests areas for future research and potential performance improvement. The results of this study contribute to athletes’ self-monitoring and coaching strategies.
Depth-First Net Unfoldings and Equivalent Reduction
Authors: Yang Xu , Ye Chen , Chen Yijun
Published: September 2023

In Petri net unfolding, according to the strategies of breadth first and depth first, the biggest problem lies in the potential explosion of the state space. Unfolding generates either accessible trees or branch processes. Making marking reduction or branch cutting accessible proves to be an effective approach to mitigating the state space expansion. In this paper, we propose three reduction rules based on similarity equivalence, conduct state space reduction, present three theorems supported by a case study, and propose a new unfolding algorithm for the unfolding process. In both the new case and the experiments, the completeness, optimality, completeness, and memory and time consumption are reduced by about 60%.
Automatic Trajectory Determination in Automated Robotic Welding Considering Weld Joint Symmetry
Authors: Curiel David , Veiga Fernando , Suarez Alfredo , Villanueva Pedro , Aldalur Eider
Published: September 2023

The field of inspection for welded structures is currently in a state of rapid transformation driven by a convergence of global technological, regulatory, and economic factors. This evolution is propelled by several key drivers, including the introduction of novel materials and welding processes, continuous advancements in inspection technologies, innovative approaches to weld acceptance code philosophy and certification procedures, growing demands for cost-effectiveness and production quality, and the imperative to extend the lifespan of aging structures. Foremost among the challenges faced by producers today is the imperative to meet customer demands, which entails addressing both their explicit and implicit needs. Furthermore, the integration of emerging materials and technologies necessitates the exploration of fresh solutions. These solutions aim to enhance inspection process efficiency while providing precise quantitative insights into defect identification and location. To this end, our project proposes cutting-edge technologies, some of which have yet to gain approval within the sector. Noteworthy among these innovations is the integration of vision systems into welding robots, among other solutions. This paper introduces a groundbreaking algorithm for tool path selection, leveraging profile scanning and the concept of joint symmetry. The application of symmetry principles for trajectory determination represents a pioneering approach within this expansive field.
Synthesis and Structure of a New Iodate Cs5[Sc2(IO3)9](IO3)2 with a Complex Framework Based on the Condensation of [Sc(IO3)6] Building Blocks
Authors: Reutova Olga V. , Belokoneva Elena L. , Volkov Anatoly S. , Dimitrova Olga V.
Published: September 2023

Transparent single crystals of a new iodate Cs5[Sc2(IO3)9](IO3)2 were synthesized under mild hydrothermal conditions. X-ray diffraction of single crystal was used to determine the large crystal structure with 52 independent atoms, sp. gr. P21/c. The building blocks [Sc(IO3)6]3− of Sc octahedra and six (IO3) groups are condensed into a framework; the structure contains additional isolated (IO3) groups. Large Cs cations occupy holes and form an intercrossing framework. Similar building blocks [M(IO3)6] are known for many iodates; however, most are isolated. Their topology and symmetry differ and determine the properties of the compounds.
Comparing Performance and Preference of Visually Impaired Individuals in Object Localization: Tactile, Verbal, and Sonification Cueing Modalities
Authors: Abu Rass Shatha , Cohen Omer , Bareli Eliav , Portnoy Sigal
Published: September 2023

Audio guidance is a common means of helping visually impaired individuals to navigate, thereby increasing their independence. However, the differences between different guidance modalities for locating objects in 3D space have yet to be investigated. The aim of this study was to compare the time, the hand’s path length, and the satisfaction levels of visually impaired individuals using three automatic cueing modalities: pitch sonification, verbal, and vibration. We recruited 30 visually impaired individuals (11 women, average age 39.6 ± 15.0), who were asked to locate a small cube, guided by one of three cueing modalities: sonification (a continuous beep that increases in frequency as the hand approaches the cube), verbal prompting (“right”, “forward”, etc.), and vibration (via five motors, attached to different locations on the hand). The three cueing modalities were automatically activated by computerized motion capture systems. The subjects separately answered satisfaction questions for each cueing modality. The main finding was that the time to find the cube was longer using the sonification cueing (p = 0.016). There were no significant differences in the hand path length or the subjects’ satisfaction. It can be concluded that verbal guidance may be the most effective for guiding people with visual impairment to locate an object in a 3D space.
Multi-Classification of Lung Infections Using Improved Stacking Convolution Neural Network
Authors: Bhimavarapu Usharani , Chintalapudi Nalini , Battineni Gopi
Published: September 2023

Lung disease is a respiratory disease that poses a high risk to people worldwide and includes pneumonia and COVID-19. As such, quick and precise identification of lung disease is vital in medical treatment. Early detection and diagnosis can significantly reduce the life-threatening nature of lung diseases and improve the quality of life of human beings. Chest X-ray and computed tomography (CT) scan images are currently the best techniques to detect and diagnose lung infection. The increase in the chest X-ray or CT scan images at the time of training addresses the overfitting dilemma, and multi-class classification of lung diseases will deal with meaningful information and overfitting. Overfitting deteriorates the performance of the model and gives inaccurate results. This study reduces the overfitting issue and computational complexity by proposing a new enhanced kernel convolution function. Alongside an enhanced kernel convolution function, this study used convolution neural network (CNN) models to determine pneumonia and COVID-19. Each CNN model was applied to the collected dataset to extract the features and later applied these features as input to the classification models. This study shows that extracting deep features from the common layers of the CNN models increased the performance of the classification procedure. The multi-class classification improves the diagnostic performance, and the evaluation metrics improved significantly with the improved support vector machine (SVM). The best results were obtained using the improved SVM classifier fed with the features provided by CNN, and the success rate of the improved SVM was 99.8%.
A Birth Cohort Follow-Up Study on Congenital Zika Virus Infection in Vietnam
Authors: Toizumi Michiko , Vu Cuong Nguyen , Huynh Hai Thi , Uematsu Masafumi , Tran Vy Thao , Vo Hien Minh , Nguyen Hien Anh Thi , Ngwe Tun Mya Myat , Bui Minh Xuan , Dang Duc Anh , Moriuchi Hiroyuki , Yoshida Lay-Myint
Published: September 2023

We assessed the development, sensory status, and brain structure of children with congenital Zika virus (ZIKV) infection (CZI) at two years and preschool age. CZI was defined as either ZIKV RNA detection or positive ZIKV IgM and neutralization test in the cord or neonatal blood. Twelve children with CZI born in 2017–2018 in Vietnam, including one with Down syndrome, were assessed at 23–25.5 months of age, using Ages and Stages Questionnaire (ASQ-3), ASQ:Social-Emotional (ASQ:SE-2), Modified Checklist for Autism in Toddlers, automated auditory brainstem response (AABR), and Spot Vision Screener (SVS). They underwent brain CT and MRI. They had detailed ophthalmological examinations, ASQ-3, and ASQ:SE-2 at 51–62 months of age. None had birthweight or head circumference z-score < −3 except for the one with Down syndrome. All tests passed AABR (n = 10). No ophthalmological problems were detected by SVS (n = 10) and detailed examinations (n = 6), except for a girl’s astigmatism. Communication and problem-solving domains in a boy at 24 months, gross-motor area in a boy, and gross-motor and fine-motor areas in another boy at 59–61 months were in the referral zone. Brain CT (n = 8) and MRI (n = 6) revealed no abnormalities in the cerebrum, cerebellum, or brainstem other than cerebellar hypoplasia with Down syndrome. The CZI children were almost age-appropriately developed with no brain or eye abnormalities. Careful and longer follow-up is necessary for children with CZI.
Kinetics of TTV Loads in Peripheral Blood Mononuclear Cells of Early Treated Acute HIV Infections
Authors: Abbate Isabella , Rozera Gabriella , Cimini Eleonora , Carletti Fabrizio , Tartaglia Eleonora , Rubino Marika , Pittalis Silvia , Esvan Rozenn , Gagliardini Roberta , Mondi Annalisa , Mazzotta Valentina , Camici Marta , Girardi Enrico , Vaia Francesco , Puro Vincenzo , Antinori Andrea , Maggi Fabrizio
Published: September 2023

Torquetenovirus (TTV) is the most abundant component of the human blood virome and its replication is controlled by a functioning immune system. In this study, TTV replication was evaluated in 21 people with acute HIV infection (AHI) and immune reconstitution following antiretroviral therapy (ART). PBMC-associated TTV and HIV-1 DNA, as well as plasma HIV-1 RNA, were measured by real-time PCR. CD4 and CD8 differentiation, activation, exhaustion, and senescence phenotypes were analyzed by flow cytometry. Thirteen healthy donors (HD) and twenty-eight chronically infected HIV individuals (CHI), late presenters at diagnosis, were included as control groups. TTV replication in AHI seems to be controlled by the immune system being higher than in HD and lower than in CHI. During ART, a transient increase in TTV DNA levels was associated with a significant perturbation of activation and senescence markers on CD8 T cells. TTV loads were positively correlated with the expansion of CD8 effector memory and CD57+ cells. Our results shed light on the kinetics of TTV replication in the context of HIV acute infection and confirm that the virus replication is strongly regulated by the modulation of the immune system.
Treatment Emergent Dolutegravir Resistance Mutations in Individuals Naïve to HIV-1 Integrase Inhibitors: A Rapid Scoping Review
Authors: Tao Kaiming , Rhee Soo-Yon , Chu Carolyn , Avalos Ava , Ahluwalia Amrit K. , Gupta Ravindra K. , Jordan Michael R. , Shafer Robert W.
Published: September 2023

Background: Dolutegravir (DTG)-based antiretroviral therapy (ART) rarely leads to virological failure (VF) and drug resistance in integrase strand transfer inhibitor (INSTI)-naïve persons living with HIV (PLWH). As a result, limited data are available on INSTI-associated drug resistance mutations (DRMs) selected by DTG-containing ART regimens. Methods: We reviewed studies published through July 2023 to identify those reporting emergent major INSTI-associated DRMs in INSTI-naïve PLWH receiving DTG and those containing in vitro DTG susceptibility results using a standardized assay. Results: We identified 36 publications reporting 99 PLWH in whom major nonpolymorphic INSTI-associated DRMs developed on a DTG-containing regimen and 21 publications containing 269 in vitro DTG susceptibility results. DTG-selected DRMs clustered into four largely non-overlapping mutational pathways characterized by mutations at four signature positions: R263K, G118R, N155H, and Q148H/R/K. Eighty-two (82.8%) viruses contained just one signature DRM, including R263K (n = 40), G118R (n = 24), N155H (n = 9), and Q148H/R/K (n = 9). Nine (9.1%) contained ≥1 signature DRM, and eight (8.1%) contained just other DRMs. R263K and G118R were negatively associated with one another and with N155H and Q148H/K/R. R263K alone conferred a median 2.0-fold (IQR: 1.8–2.2) reduction in DTG susceptibility. G118R alone conferred a median 18.8-fold (IQR:14.2–23.4) reduction in DTG susceptibility. N155H alone conferred a median 1.4-fold (IQR: 1.2–1.6) reduction in DTG susceptibility. Q148H/R/K alone conferred a median 0.8-fold (IQR: 0.7–1.1) reduction in DTG susceptibility. Considerably higher levels of reduced susceptibility often occurred when signature DRMs occurred with additional INSTI-associated DRMs. Conclusions: Among INSTI-naïve PLWH with VF and treatment emergent INSTI-associated DRMs, most developed one of four signature DRMs, most commonly R263K or G118R. G118R was associated with a much greater reduction in DTG susceptibility than R263K.
Prevalence and Impact on Mortality of Colonization and Super-Infection by Carbapenem-Resistant Gram-Negative Organisms in COVID-19 Hospitalized Patients
Authors: Casale Roberto , Bianco Gabriele , Bastos Paulo , Comini Sara , Corcione Silvia , Boattini Matteo , Cavallo Rossana , Rosa Francesco Giuseppe De , Costa Cristina
Published: September 2023

Background: The relationship between superinfection by multidrug-resistant Gram-negative bacteria and mortality among SARS-CoV-2 hospitalized patients is still unclear. Carbapenem-resistant Acinetobacter baumannii and carbapenemase-producing Enterobacterales are among the most frequently isolated species when it comes to hospital-acquired superinfections among SARS-CoV-2 patients. Methods: Herein, a retrospective study was carried out using data from adult patients hospitalized for COVID-19. The interaction between in-hospital mortality and rectal carriage and superinfection by carbapenemase-producing Enterobacterales and/or carbapenem-resistant Acinetobacter baumannii was assessed. Results: The incidence of KPC-producing Klebsiella pneumoniae and/or carbapenem-resistant Acinetobacter baumannii rectal carriage was 30%. Bloodstream infection and/or pneumonia due to KPC-producing Klebsiella pneumoniae and/or carbapenem-resistant Acinetobacter baumannii occurred in 20% of patients. A higher Charlson comorbidity index (OR 1.41, 95% CI 1.24–1.59), being submitted to invasive mechanical ventilation/ECMO ≥ 96 h (OR 6.34, 95% CI 3.18–12.62), being treated with systemic corticosteroids (OR 4.67, 95% CI 2.43–9.05) and having lymphopenia at the time of admission (OR 0.54, 95% CI 0.40–0.72) were the features most strongly associated with in-hospital mortality. Conclusions: Although KPC-producing Klebsiella pneumoniae and/or carbapenem-resistant Acinetobacter baumannii rectal carriage, and/or bloodstream infection/pneumonia were diagnosed in a remarkable percentage of COVID-19 patients, their impact on in-hospital mortality was not significant. Further studies are needed to assess the burden of antimicrobial resistance as a legacy of COVID-19 in order to identify future prevention opportunities.
E3 Ubiquitin Ligases in Gammaherpesviruses and HIV: A Review of Virus Adaptation and Exploitation
Authors: Oswald Jessica , Constantine Mathew , Adegbuyi Adedolapo , Omorogbe Esosa , Dellomo Anna J. , Ehrlich Elana S.
Published: September 2023

For productive infection and replication to occur, viruses must control cellular machinery and counteract restriction factors and antiviral proteins. Viruses can accomplish this, in part, via the regulation of cellular gene expression and post-transcriptional and post-translational control. Many viruses co-opt and counteract cellular processes via modulation of the host post-translational modification machinery and encoding or hijacking kinases, SUMO ligases, deubiquitinases, and ubiquitin ligases, in addition to other modifiers. In this review, we focus on three oncoviruses, Epstein–Barr virus (EBV), Kaposi’s sarcoma herpesvirus (KSHV), and human immunodeficiency virus (HIV) and their interactions with the ubiquitin–proteasome system via viral-encoded or cellular E3 ubiquitin ligase activity.
Multi-epitope Antigen for Specific Serological Detection of Dengue Viruses
Authors: Pereira Samuel Santos , Andreata-Santos Robert , de Castro-Amarante Maria Fernanda , Venceslau-Carvalho Aléxia Adrianne , Sales Natiely Silva , Silva Mariângela de Oliveira , Alves Rúbens Prince dos Santos , Jungmann Patrícia , Ferreira Luís Carlos de Souza
Published: September 2023

Dengue is an infectious disease of global health concern that continues to require surveillance. Serological testing has been used to investigate dengue-infected patients, but specificity is affected by the co-circulation of ZIKA virus (ZIKV), which shares extensive antigen similarities. The goal of this study was the development of a specific dengue virus (DENV) IgG ELISA based on a multi-epitope NS1-based antigen for antibody detection. The multi-epitope protein (T-ΔNS1), derived from a fragment of the NS1-protein of the four DENV serotypes, was expressed in Escherichia coli and purified via affinity chromatography. The antigenicity and specificity were evaluated with sera of mice infected with DENV-1–4 or ZIKV or after immunization with the recombinant ΔNS1 proteins. The performance of the T-ΔNS1-based IgG ELISA was also determined with human serum samples. The results demonstrate that the DENV T-ΔNS1 was specifically recognized by the serum IgG of dengue-infected mice or humans but showed no or reduced reactivity with ZIKV-infected subjects. Based on the available set of clinical samples, the ELISA based on the DENV T-ΔNS1 achieved 77.42% sensitivity and 88.57% specificity. The results indicate that the T-ΔNS1 antigen is a promising candidate for the development of specific serological analysis.
Real-Time Analysis of SARS-CoV-2-Induced Cytolysis Reveals Distinct Variant-Specific Replication Profiles
Authors: Scheuermann Sarah E. , Goff Kelly , Rowe Lori A. , Beddingfield Brandon J. , Maness Nicholas J.
Published: September 2023

The ability of each new SARS-CoV-2 variant to evade host humoral immunity is the focus of intense research. Each variant may also harbor unique replication capabilities relevant for disease and transmission. Here, we demonstrate a new approach to assessing viral replication kinetics using real-time cell analysis (RTCA). Virus-induced cell death is measured in real time as changes in electrical impedance through cell monolayers while images are acquired at defined intervals via an onboard microscope and camera. Using this system, we quantified replication kinetics of five clinically important viral variants: WA1/2020 (ancestral), Delta, and Omicron subvariants BA.1, BA.4, and BA.5. Multiple measures proved useful in variant replication comparisons, including the elapsed time to, and the slope at, the maximum rate of cell death. Important findings include significantly weaker replication kinetics of BA.1 by all measures, while BA.5 harbored replication kinetics at or near ancestral levels, suggesting evolution to regain replicative capacity, and both an altered profile of cell killing and enhanced fusogenicity of the Delta variant. Together, these data show that RTCA is a robust method to assess replicative capacity of any given SARS-CoV-2 variant rapidly and quantitatively, which may be useful in assessment of newly emerging variants.
Developing an Indirect ELISA for the Detection of African Swine Fever Virus Antibodies Using a Tag-Free p15 Protein Antigen
Authors: Wu Zhi , Lu Huipeng , Zhu Dewei , Xie Jun , Sun Fan , Xu Yan , Zhang Hua , Wu Zhijun , Xia Wenlong , Zhu Shanyuan
Published: September 2023

African swine fever (ASF) is one of the most severe diseases caused by the ASF virus (ASFV), causing massive economic losses to the global pig industry. Serological tests are important in ASF epidemiological surveillance, and more antigen targets are needed to meet market demand for ASFV antibody detection. In the present study, ASFV p15 protein was fusion-expressed in Escherichia coli (E. coli) with elastin-like polypeptide (ELP), and the ELP-p15 protein was purified using a simple inverse transition cycling (ITC) process. The ELP tag was cleaved off using tobacco etch virus protease (TEVp), resulting in a tag-free p15 protein. Western blot analysis demonstrated that the p15 protein reacted strongly with ASFV-positive serum. The p15 protein was used as a coating antigen in an indirect ELISA (iELISA) for detecting ASFV antibodies. The p15-iELISA method demonstrated high specificity to ASFV-positive sera, with a maximum detection dilution of 1:1600. Moreover, the method exhibited good reproducibility, with less intra-assay and inter-assay CV values than 10%. Therefore, p15-iELISA offers a novel approach for accurately detecting ASFV antibodies with significant clinical application potential.
Passive Sampler Technology for Viral Detection in Wastewater-Based Surveillance: Current State and Nanomaterial Opportunities
Authors: Aguayo-Acosta Alberto , Jiménez-Rodríguez Mildred G. , Silva-Lance Fernando , Oyervides-Muñoz Mariel Araceli , Armenta-Castro Arnoldo , de la Rosa Orlado , Ovalle-Carcaño Antonio , Melchor-Martínez Elda M. , Aghalari Zahra , Parra-Saldívar Roberto , Sosa-Hernández Juan Eduardo
Published: September 2023

Although wastewater-based surveillance (WBS) is an efficient community-wide surveillance tool, its implementation for pathogen surveillance remains limited by ineffective sample treatment procedures, as the complex composition of wastewater often interferes with biomarker recovery. Moreover, current sampling protocols based on grab samples are susceptible to fluctuant biomarker concentrations and may increase operative costs, often rendering such systems inaccessible to communities in low-to-middle-income countries (LMICs). As a response, passive samplers have emerged as a way to make wastewater sampling more efficient and obtain more reliable, consistent data. Therefore, this study aims to review recent developments in passive sampling technologies to provide researchers with the tools to develop novel passive sampling strategies. Although promising advances in the development of nanostructured passive samplers have been reported, optimization remains a significant area of opportunity for researchers in the area, as methods for flexible, robust adsorption and recovery of viral genetic materials would greatly improve the efficacy of WBS systems while making them more accessible for communities worldwide.
Stimulation of Potent Humoral and Cellular Immunity via Synthetic Dual-Antigen MVA-Based COVID-19 Vaccine COH04S1 in Cancer Patients Post Hematopoietic Cell Transplantation and Cellular Therapy
Authors: Chiuppesi Flavia , Ortega-Francisco Sandra , Gutierrez Miguel-Angel , Li Jing , Ly Minh , Faircloth Katelyn , Mack-Onyeike Jada , La Rosa Corinna , Thomas Sandra , Zhou Qiao , Drake Jennifer , Slape Cynthia , Fernando Paolo , Rida Wasima , Kaltcheva Teodora , Grifoni Alba , Sette Alessandro , Patterson Angela , Dempsey Shannon , Ball Brian , Ali Haris , Salhotra Amandeep , Stein Anthony , Nathwani Nitya , Rosenzweig Michael , Nikolaenko Liana , Al Malki Monzr M. , Dickter Jana , Nanayakkara Deepa D. , Puing Alfredo , Forman Stephen J. , Taplitz Randy A. , Zaia John A. , Nakamura Ryotaro , Wussow Felix , Diamond Don J. , Dadwal Sanjeet S.
Published: September 2023

Hematopoietic cell transplantation (HCT) and chimeric antigen receptor (CAR)-T cell patients are immunocompromised, remain at high risk following SARS-CoV-2 infection, and are less likely than immunocompetent individuals to respond to vaccination. As part of the safety lead-in portion of a phase 2 clinical trial in patients post HCT/CAR-T for hematological malignancies (HM), we tested the immunogenicity of the synthetic modified vaccinia Ankara-based COVID-19 vaccine COH04S1 co-expressing spike (S) and nucleocapsid (N) antigens. Thirteen patients were vaccinated 3–12 months post HCT/CAR-T with two to four doses of COH04S1. SARS-CoV-2 antigen-specific humoral and cellular immune responses, including neutralizing antibodies to ancestral virus and variants of concern (VOC), were measured up to six months post vaccination and compared to immune responses in historical cohorts of naïve healthy volunteers (HV) vaccinated with COH04S1 and naïve healthcare workers (HCW) vaccinated with the FDA-approved mRNA vaccine Comirnaty® (Pfizer, New York, NY, USA). After one or two COH04S1 vaccine doses, HCT/CAR-T recipients showed a significant increase in S- and N-specific binding antibody titers and neutralizing antibodies with potent activity against SARS-CoV-2 ancestral virus and VOC, including the highly immune evasive Omicron XBB.1.5 variant. Furthermore, vaccination with COH04S1 resulted in a significant increase in S- and N-specific T cells, predominantly CD4+ T lymphocytes. Elevated S- and N-specific immune responses continued to persist at six months post vaccination. Furthermore, both humoral and cellular immune responses in COH04S1-vaccinated HCT/CAR-T patients were superior or comparable to those measured in COH04S1-vaccinated HV or Comirnaty®-vaccinated HCW. These results demonstrate robust stimulation of SARS-CoV-2 S- and N-specific immune responses including cross-reactive neutralizing antibodies by COH04S1 in HM patients post HCT/CAR-T, supporting further testing of COH04S1 in immunocompromised populations.
The Effectiveness of Paracetamol to Reduce the Post-Vaccination SARS-CoV-2 Adverse Effects in an Italian Vaccination Center
Authors: Ricci Eleonora , Glavasc Anamaria , Morandini Barbara , Grassi Maria Caterina , La Torre Giuseppe
Published: September 2023

The arrival of specific vaccines was crucial for the eradication of COVID-19. Despite the security of the vaccination, the administration of COVID-19 mRNA vaccines often causes systemic side effects for a short time after the injection, such as headache, fatigue, fever, muscle pain and nausea. These side effects can limit the adherence to COVID-19 vaccines administration, especially in healthcare workers. This study aims to analyze the impact of the prophylactic use of paracetamol to reduce the post-vaccination Comirnaty/Pfizer adverse effects. The study took place at the San Giovanni Battista Hospital in Rome and included all hospital employees who received two doses of Pfizer-BioNTech. The vaccination health personnel recommended the preventive intake of 1 g of paracetamol before the inoculation of the vaccine and then every 6 h in the first 24 h. Information was collected through forms and subsequent telephone recall. A total of 403 volunteers were involved in the study, with 391 of them receiving two doses and twelve individuals only one dose of the vaccine. The main results demonstrated that the prophylactic therapy influenced the lower onset of asthenia in the first and second doses. However, there were no significant differences between the two groups in terms of fever, headache and localized pain. Paracetamol had a good impact on the side effect of COVID-19 vaccination, reducing asthenia in both doses and mitigating the total of symptoms during the second vaccination.
Natural Antibodies Produced in Vaccinated Patients and COVID-19 Convalescents Recognize and Hydrolyze Oligopeptides Corresponding to the S-Protein of SARS-CoV-2
Authors: Timofeeva Anna M. , Sedykh Sergey E. , Sedykh Tatyana A. , Nevinsky Georgy A.
Published: September 2023

The S-protein is the major antigen of the SARS-CoV-2 virus, against which protective antibodies are generated. The S-protein gene was used in adenoviral vectors and mRNA vaccines against COVID-19. While the primary function of antibodies is to bind to antigens, catalytic antibodies can hydrolyze various substrates, including nucleic acids, proteins, oligopeptides, polysaccharides, and some other molecules. In this study, antibody fractions with affinity for RBD and S-protein (RBD-IgG and S-IgG) were isolated from the blood of COVID-19 patients vaccinated with Sputnik V. The fractions were analyzed for their potential to hydrolyze 18-mer oligopeptides corresponding to linear fragments of the SARS-CoV-2 S-protein. Here, we show that the IgG antibodies hydrolyze six out of nine oligopeptides efficiently, with the antibodies of COVID-19-exposed donors demonstrating the most significant activity. The IgGs of control donors not exposed to SARS-CoV-2 were found to be inactive in oligopeptide hydrolysis. The antibodies of convalescents and vaccinated patients were found to hydrolyze oligopeptides in a wide pH range, with the optimal pH range between 6.5 and 7.5. The hydrolysis of most oligopeptides by RBD-IgG antibodies is inhibited by thiol protease inhibitors, whereas S-IgG active centers generally combine several types of proteolytic activities. Ca2+ ions increase the catalytic activity of IgG preparations containing metalloprotease-like active centers. Thus, the proteolytic activity of natural antibodies against the SARS-CoV-2 protein is believed to be due to the similarity of catalytic antibodies’ active centers to canonical proteases. This work raises the question of the possible physiological role of proteolytic natural RBD-IgG and S-IgG resulting from vaccination and exposure to COVID-19.
Vaccinating Welders against Pneumococcus: Evidence from a Systematic Review and Meta-Analysis
Authors: Riccò Matteo , Ferraro Pietro , Zaffina Salvatore , Camisa Vincenzo , Marchesi Federico , Gori Davide
Published: September 2023

Workers occupationally exposed to welding dusts and fumes have been suspected to be at increased risk of invasive pneumococcal disease (IPD). Since the 2010s, the United Kingdom Department of Health and the German Ständige Impfkommission (STIKO) actively recommend welders undergo immunization with the 23-valent polysaccharide (PPV23) pneumococcal vaccine, but this recommendation has not been extensively shared by international health authorities. The present meta-analysis was therefore designed to collect available evidence on the occurrence of pneumococcal infection and IPD among welders and workers exposed to welding fumes, in order to ascertain the effective base of evidence for this recommendation. PubMed, Embase and MedRxiv databases were searched without a timeframe restriction for the occurrence of pneumococcal infections and IPD among welders and workers exposed to metal dusts, and articles meeting the inclusion criteria were included in a random-effect meta-analysis model. From 854 entries, 14 articles (1.6%) underwent quantitative analysis, including eight retrospective studies (publication range: 1980–2010), and six reports of professional clusters in shipbuilding (range: 2017–2020). Welders had an increased likelihood of developing IPD compared with non-welders (odds ratio 2.59, 95% CI 2.00–3.35, I2 = 0%, p = 0.58), and an increased likelihood of dying from IPD (standardized mortality ratio (SMR) 2.42, 95% CI 1.96-2.99, I2 = 0%, p = 0.58). Serotype typing was available for 72 cases, 60.3% of which were represented by serotype 4, followed by 12F (19.2%) and serotype 8 (8.2%). Although the available data derive from a limited number of studies, available results suggest that pneumococcal vaccination should be recommended for workers exposed to welding fumes, and vaccination strategies should consider the delivery of recombinant formulates in order to combine the direct protection against serotypes of occupational interest with the mucosal immunization, reducing the circulation of the pathogen in occupational settings characterized by close interpersonal contact.
Strategies and Trends in COVID-19 Vaccination Delivery: What We Learn and What We May Use for the Future
Authors: Tradigo Giuseppe , Das Jayanta Kumar , Vizza Patrizia , Roy Swarup , Guzzi Pietro Hiram , Veltri Pierangelo
Published: September 2023

Vaccination has been the most effective way to control the outbreak of the COVID-19 pandemic. The numbers and types of vaccines have reached considerable proportions, even if the question of vaccine procedures and frequency still needs to be resolved. We have come to learn the necessity of defining vaccination distribution strategies with regard to COVID-19 that could be used for any future pandemics of similar gravity. In fact, vaccine monitoring implies the existence of a strategy that should be measurable in terms of input and output, based on a mathematical model, including death rates, the spread of infections, symptoms, hospitalization, and so on. This paper addresses the issue of vaccine diffusion and strategies for monitoring the pandemic. It provides a description of the importance and take up of vaccines and the links between procedures and the containment of COVID-19 variants, as well as the long-term effects. Finally, the paper focuses on the global scenario in a world undergoing profound social and political change, with particular attention on current and future health provision. This contribution would represent an example of vaccination experiences, which can be useful in other pandemic or epidemiological contexts.
A Field Efficacy Trial of Recombinant Porcine Circovirus Type 2d Vaccine in Three Herds
Authors: Ju Lanjeong , Jayaramaiah Usharani , Lee Min-A , Jeong Young-Ju , You Su-Hwa , Lee Hyang-Sim , Hyun Bang-Hun , Lee Nakhyung , Kang Seok-Jin
Published: September 2023

This study aimed to evaluate the efficacy of a virus-like particle (VLP) vaccine containing the open reading frame 2 of porcine circovirus type 2d (PCV2d) in a farm environment where natural infections associated with porcine circovirus-associated disease are endemic. The vaccine trial was conducted on three farms (H, M, and Y) with a history of infections including porcine reproductive and respiratory syndrome virus (PRRSV), PCV, Mycoplasma, and E. coli. Farm H, as well as farms M and Y, experienced natural PCV2 infection between 4 and 8 weeks post-vaccination (wpv), and 8 and 12 wpv, respectively. Viremia levels of all farms were significantly (p < 0.05) lower in vaccinated piglets than the control group after natural infection. In all farms, serum immunoglobulin G levels peaked at 8 wpv in the vaccinated groups, surpassing those in the control groups. Furthermore, neutralizing antibody titers were significantly (p < 0.05) higher in the vaccinated groups than the control groups in farms H and Y (0–8 wpv). However, there were no significant differences between the vaccinated and control group in neutralizing antibody titers of farm M (0–20 wpv). In terms of body weight, vaccinated piglets from all three farms showed significantly increased average weights at 12 wpv compared to the control groups. In conclusion, our study revealed noteworthy differences in viremia and body weight gain between vaccinated and control animals on three farms. As a result, this field trial of PCV2d VLP vaccine was successful in protecting piglets from natural PCV2 infection.
Foveal and Retinal Nerve Fiber Layer Thickness in Children With a History of Retinopathy of Prematurity Treated With Bevacizumab: A Cross-sectional Study
Authors: Eftekhari Milani Amir , Javani Bahareh , Ahoor Mohamadhosein , Abdi Shahshahani Mehdi , Farhadi Fereshteh
Published: September 2023

Objectives: This study aimed to investigate the effects of bevacizumab (an anti-VEGF antibody) on the structure of retina such as the thickness of the nerve fiber layer and macula with optical coherence tomography (OCT) in retinopathy of prematurity (ROP) patients. Materials and Methods: This cross-sectional study was conducted in 2021-2022 in Tabriz, Iran to investigate preterm children aged 4-6 years. To this end, a total of 300 eyes of 150 children were assigned to three groups and then were examined by performing Fundus examination of retina, eye refraction test, and OCT imaging. The first group included 100 eyes with a history of ROP and treated with bevacizumab, the second group consisted of 100 eyes with a history of ROP but improved spontaneously, and the third group comprised 100 eyes of preterm children without a history of ROP. Results: Foveal thickness was 235.46 ± 11 µm in the group receiving bevacizumab, 267.70 ± 12 µm in the group with spontaneous improvement of ROP, and 269.48 ± 10 µm in the group with no ROP. Therefore, the mean thickness of the fovea in the group receiving bevacizumab was significantly lower than that in the other two groups. In the group receiving bevacizumab, moreover, the thickness of the nerve layer of the retina was less than that in the other two groups (P<0.005). Conclusions: In sum, a greater understanding of the development of premature fovea was achieved, which may have facilitated identifying the possible clinical and long-term effects of these abnormal changes on adulthood visual acuity. It was recommended that further studies should be carried out to compare laser-treated eyes in order for expanding the scientific understanding of the development of premature fovea and other aspects of ROP, which may have decreased the preventable blindness rate.
Comparison of the Effects of Fluid Therapy With Normal Saline, Ringer, and Ringer’s Lactate During Anesthesia on the Hemodynamic Status and Events During Orthopedic Lower Limb Orthopedics Surgeries
Authors: Mortazavi Mirmohammadtaghi , Moharrami Mohammadreza , Nazari Behrooz , Mohammadipour Anvari Hassan
Published: September 2023

Objectives: Given that no study has so far carefully examined the effects and benefits of crystalloids on the hemodynamic status and events during the surgery for lower limb orthopedic surgeries, the current study aimed to compare fluid therapy with normal saline (NS), Ringer (R), and Ringer’s lactate (RL) during anesthesia on the hemodynamic status and events during lower limb elective orthopedic surgeries. Materials and Methods: This cross-sectional descriptive study was conducted in 2019 with the participation of 270 patients (three groups each including 90 subjects) who were scheduled for an elective lower limb orthopedic surgery in Imam Reza hospital of Tabriz. Patients received one of these serums of NS, R, and RL. Electrolytes were measured before and after the surgery. Finally, data were analyzed using ANOVA and chi-square tests. Results: Based on the results, there was no significant difference between the three groups in terms of hemoglobin, hematocrit, sodium, calcium, potassium, chlorine, albumin, creatinine, urea, bicarbonate, pH, and arterial oxygen levels (P>0.05), and RL serum had the least effect on all factors. Conclusions: In general, the administration of NS, R, and RL in lower limb orthopedic surgeries did not result in significant differences although RL had the least adverse effects and was associated with better results.
Preservation of Testicular Tissue and Alleviation of Oxidative Stress by Carvacrol Following Torsion/ Detorsion in Adult Male Rats
Authors: Khatami Arman , Rahmanifar Ghazal , Khaki Arash , Teymoori Afshin , Rezaii Ali
Published: September 2023

Objectives: Testicular torsion is a critical medical condition necessitating prompt diagnosis and intervention. This study aimed to explore the potential safeguarding effect of carvacrol against histological damage and oxidative stress resulting from torsion/detorsion (T/D) in rat testes. Materials and Methods: A total of 32 adult male rats were randomly divided into four groups. The sham group did not undergo any intervention. The second group received an intraperitoneal injection of 75 mg/kg carvacrol half an hour before detorsion application. The third group was administered 80 mg/kg carvacrol intraperitoneally without detorsion. The fourth group (control) experienced (T/D) through the administration of saline. Following a 5-hour reperfusion period, the left testis was excised for histological slide preparation. Blood serum was used to measure antioxidant enzyme levels. Data analysis was performed using the SPSS version 19 software and analysis of variance tests. Results: Significant histological alterations were observed between the sham and other three groups. The levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and testosterone significantly decreased in all treatment groups compared to the control group (P<0.05). Conversely, malondialdehyde (MDA) levels increased in the torsion control group in contrast to the sham group (P<0.05). Carvacrol administration mitigated MDA levels in the treatment groups. Also, there were significant differences in tissue parameters between the sham and the other groups (P<0.05). Conclusions: According to the results of this study, carvacrol possesses the potential to mitigate testicular tissue damage, enhance testicular function, and ameliorate oxidative stress consequential to testicular rotation.
Development and Psychometric Evaluation of the Sexual and Reproductive Health Questionnaire for Women With Type 1 Diabetes Mellitus: A Study Protocol for a Sequential Exploratory Mixed-Methods Study
Authors: Azimi Nasrin , Ozgoli Giti , Ebadi Abbas , Alavi Majd Hamid , Rajab Assadollah , Jalali Chimeh Fatemeh , Kazemi Samiyeh
Published: September 2023

Objectives: Type 1 diabetes mellitus (T1DM) can profoundly influence different dimension of health such as sexual and reproductive health (SRH). The aim of this study is to develop and evaluate the psychometric properties of the Sexual and Reproductive Health Questionnaire for Women with Type 1 Diabetes Mellitus (SRHQ-WT1DM). Materials and Methods: This protocol for a sequential exploratory mixed-methods study has two phases. Conducting a literature review, the first phase of the study will develop the items of SRHQ-WT1DM. Data analysis in this phase will be performed using directed content analysis and according to the framework proposed by the United Nations Population Fund (UNFPA) and the World Health Organization (WHO) for SRH in women. The second phase will evaluate the psychometric properties of SRHQWT1DM through a methodological study. Face and content validity will be evaluated both qualitatively and quantitatively, and construct validity will be done by exploratory factor analysis and convergent validity. The questionnaire’s reliability and stability will be examined using internal consistency (by Cronbach’s alpha calculation) and test-retest method (by Intra class correlation coefficient calculation), respectively. Descriptive statistics, Pearson correlation coefficient, and independent t-test will also be used. Women with T1DM who are of childbearing age and have unknown mental disorders will be selected as participants in the study from the Iranian Diabetes Society. Discussion: Development and psychometric evaluation of a specific tool for SRH assessment in women will help identify and fulfill their SRH-related needs and improve dimensions of their SRH.
Clinico-pathological Features and Survival Time of Papillary Thyroid Carcinoma in Patients With and Without Hashimoto’s Thyroiditis: A Cross-sectional Study
Authors: Moghimi Mansour , Shamsadini Ezabadi Fateme Sadat , Mortazavi Zadeh Seyed Mohammadreza , Khalilzade Saead Hossein
Published: September 2023

Objectives: Researchers have reported different results regarding the association between Hashimoto’s disease and papillary thyroid carcinoma (PTC). Some believe that the coexistence of these diseases can lead to fewer tumor invasion and recurrence rates. This study evaluated the clinico-pathological features and survival time of PTC in patients with and without Hashimoto’s thyroiditis. Materials and Methods: In this cross-sectional study, medical records of 251 participants who underwent total or subtotal thyroidectomy due to PTC from 2012 to 2019 were reviewed. The clinico-pathological features of participants, such as age, gender, tumor stage, tumor size, lymph node involvement, metastasis, capsular invasion, single or multi-focal tumor status, and survival time were recorded from their medical records and pathology report and compared in two groups with and without Hashimoto’s thyroiditis. Results: From 251 participants, 92 (36.6%) had Hashimoto’s thyroiditis, whereas 159 (63.4%) did not show any signs of this disease. Fifteen participants in the Hashimoto group and 46 in the non-Hashimoto group had a recurrence. Although there were no significant differences between the two groups in the term of recurrence rate (P = 0.08), the mean survival time was significantly difference between the two groups (69.03 and 58.78, respectively; P = 0.038) Conclusions: Results of the study revealed that Hashimoto’s thyroiditis could increase the survival time of patients with PTC.
Investigating the Level of Troponin I and its Association With Mortality in Patients With Sepsis, Severe Sepsis, and Septic Shock
Authors: Darban Mahboubeh , Gohari Ali , Biglari Marjan , Ghorbani Raheb , Sheykhabbasi Pourya
Published: September 2023

Objectives: To investigate the level of troponin I and its association with mortality in patients with sepsis, severe sepsis, and septic shock. Materials and Methods: In general, 148 patients with sepsis, severe sepsis, and septic shock admitted to Kowsar hospital of Semnan in 2017-2019 were recruited in this study. Serum troponin levels were measured during the first 24 hours of admission. The second measurement was done in the next 48 hours, which aimed at increasing the likelihood of positive troponin over time. However, if the troponin was positive just once, the patient would be in the category of positive troponin. In addition, the patient’s prognosis was recorded within 30 days. Results: Based on the results, 27.7% had troponin levels higher than normal. The mortality rate in patients with normal and abnormal levels of troponin was 5.6% and 17.1%, respectively, which was a significant difference. The age of dead patients was significantly higher than the age of the living patients and the results showed that among the variables of gender, normal or abnormal level of troponin, age, and type of underlying infection, only age had a significant relationship with patients’ vital status. Conclusions: High levels of troponin were not effective in determining the prognosis of patients with sepsis, and only age had a significant relationship with patients’ vital status while the confirmation or rejection of this hypothesis will need further studies.