bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization Parameters of Trading System with Constant Modulus of Unit Return
Authors: Piasecki Krzysztof , Stasiak Michał Dominik
Published: August 2020

The unit return is determined as the return in the quotation currency (QCR) per the unit of base exchange medium (BEM). The main purpose is to examine the applicability of a trading system with a constant modulus of unit return (CMUR). The CMUR system supports speculative operations related to the exchange rate, given as the BEM quotation per the QCR. Premises for investment decisions are based on knowledge about the quotation dynamics described by its binary representation. This knowledge is described by a prediction table containing the conditional probability distributions of exchange rate increments. Any prediction table depends on observation range. Financial effectiveness of any CMUR system is assessed in the usual way by interest rate and risk index based on Shannon entropy. The main aim of our paper is to present algorithms which may be used for selecting effective CMUR systems. Required unit return modulus and observation range are control parameters applied for management of CMUR systems. Optimal values of these parameters are obtained by implementation of the proposed algorithm. All formal considerations are illustrated by an extensive case study linked to gold trading.
Fourier-Spectral Method for the Phase-Field Equations
Authors: Yoon Sungha , Jeong Darae , Lee Chaeyoung , Kim Hyundong , Kim Sangkwon , Lee Hyun Geun , Kim Junseok
Published: August 2020

In this paper, we review the Fourier-spectral method for some phase-field models: Allen–Cahn (AC), Cahn–Hilliard (CH), Swift–Hohenberg (SH), phase-field crystal (PFC), and molecular beam epitaxy (MBE) growth. These equations are very important parabolic partial differential equations and are applicable to many interesting scientific problems. The AC equation is a reaction-diffusion equation modeling anti-phase domain coarsening dynamics. The CH equation models phase segregation of binary mixtures. The SH equation is a popular model for generating patterns in spatially extended dissipative systems. A classical PFC model is originally derived to investigate the dynamics of atomic-scale crystal growth. An isotropic symmetry MBE growth model is originally devised as a method for directly growing high purity epitaxial thin film of molecular beams evaporating on a heated substrate. The Fourier-spectral method is highly accurate and simple to implement. We present a detailed description of the method and explain its connection to MATLAB usage so that the interested readers can use the Fourier-spectral method for their research needs without difficulties. Several standard computational tests are done to demonstrate the performance of the method. Furthermore, we provide the MATLAB codes implementation in the Appendix A.
On the Characteristic Polynomial of the Generalized k-Distance Tribonacci Sequences
Authors: Trojovský Pavel
Published: August 2020

In 2008, I. Włoch introduced a new generalization of Pell numbers. She used special initial conditions so that this sequence describes the total number of special families of subsets of the set of n integers. In this paper, we prove some results about the roots of the characteristic polynomial of this sequence, but we will consider general initial conditions. Since there are currently several types of generalizations of the Pell sequence, it is very difficult for anyone to realize what type of sequence an author really means. Thus, we will call this sequence the generalized k-distance Tribonacci sequence (Tn(k))n≥0.
Low Power Consumption 3D-Inverted Ridge Thermal Optical Switch of Graphene-Coated Polymer/Silica Hybrid Waveguide
Authors: Cao Yue , Yi Yunji , Yang Yue , Lin Baizhu , Lv Jiawen , Zhao Haowen , Wang Fei , Zhang Daming
Published: August 2020

An inverted ridge 3D thermal optical (TO) switch of a graphene-coated polymer/silica hybrid waveguide is proposed. The side electrode structure is designed to reduce the mode loss induced by the graphene film and by heating the electrode. The graphene layer is designed to be located on the waveguide to assist in the conduction of heat produced by the electrode. The inverted ridge core is fabricated by etching and spin-coating processes, which can realize the flat surface waveguide. This core improves the transfer of the graphene layer and the compatibility of the fabrication processes. Because of the opposite thermal optical coefficient of polymer and silica and the high thermal conductivity of the graphene layer, the 3D hybrid TO switch with low power consumption and fast response time is obtained. Compared with the traditional TO switch without graphene film, the power consumption of the proposed TO switch is reduced by 41.43% at the wavelength of 1550 nm, width of the core layer (a) of 3 μm, and electrode distance (d) of 4 μm. The rise and fall times of the proposed TO switch are simulated to be 64.5 μs and 175 μs with a d of 4 μm, and a of 2 μm, respectively.
First Cases of Natural Infections with Borrelia hispanica in Two Dogs and a Cat from Europe
Authors: Margos Gabriele , Pantchev Nikola , Globokar Majda , Lopez Javier , Rodon Jaume , Hernandez Leticia , Herold Heike , Salas Noelia , Civit Anna , Fingerle Volker
Published: August 2020

Canine cases of relapsing fever (RF) borreliosis have been described in Israel and the USA, where two RF species, Borrelia turicatae and Borrelia hermsii, can cause similar clinical signs to the Borrelia persica in dogs and cats reported from Israel, including fever, lethargy, anorexia, thrombocytopenia, and spirochetemia. In this report, we describe the first clinical cases of two dogs and a cat from Spain (Cordoba, Valencia, and Seville) caused by the RF species Borrelia hispanica. Spirochetes were present in the blood smears of all three animals, and clinical signs included lethargy, pale mucosa, anorexia, cachexia, or mild abdominal respiration. Laboratory findings, like thrombocytopenia in both dogs, may have been caused by co-infecting pathogens (i.e., Babesia vogeli, confirmed in one dog). Anemia was noticed in one of the dogs and in the cat. Borrelia hispanica was confirmed as an infecting agent by molecular analysis of the 16S rRNA locus. Molecular analysis of housekeeping genes and phylogenetic analyses, as well as successful in vitro culture of the feline isolate confirmed the causative agent as B. hispanica.
Assessing Options for Remediation of Contaminated Mine Site Drainage Entering the River Teign, Southwest England
Authors: Jordan Abigail , Hill Rachel , Turner Adrienne , Roberts Tyrone , Comber Sean
Published: August 2020

The river Teign in Devon has come under scrutiny for failing to meet environmental quality standards for ecotoxic metals due to past mining operations. A disused mine known as Bridford Barytes mine, has been found to contribute a significant source of Zn, Cd and Pb to the river. Recently, studies have been focused on the remediation of such mine sites using low-cost treatment methods to help reduce metal loads to the river downstream. This paper explores the metal removal efficiency of red mud, a waste product from the aluminium industry, which has proven to be an attractive low-cost treatment method for adsorbing toxic metals. Adsorption kinetics and capacity experiments reveal metal removal efficiencies of up to 70% within the first 2 h when red mud is applied in pelletized form. Further, it highlights the potential of biochar, another effective adsorbent observed to remove >90% Zn using agricultural feedstock. Compliance of the Teign has been investigated by analysing dissolved metal concentrations and bioavailable fractions of Zn to assess if levels are of environmental concern. By applying a real-world application model, this study reveals that compressed pellets and agricultural biochar offer an effective, low-cost option to reducing metal concentrations and thus improving the quality of the river Teign.
Adapting a Virtual Advisor’s Verbal Conversation Based on Predicted User Preferences: A Study of Neutral, Empathic and Tailored Dialogue
Authors: Ranjbartabar Hedieh , Richards Deborah , Bilgin Ayse Aysin , Kutay Cat , Mascarenhas Samuel
Published: August 2020

Virtual agents that improve the lives of humans need to be more than user-aware and adaptive to the user’s current state and behavior. Additionally, they need to apply expertise gained from experience that drives their adaptive behavior based on deep understanding of the user’s features (such as gender, culture, personality, and psychological state). Our work has involved extension of FAtiMA (Fearnot AffecTive Mind Architecture) with the addition of an Adaptive Engine to the FAtiMA cognitive agent architecture. We use machine learning to acquire the agent’s expertise by capturing a collection of user profiles into a user model and development of agent expertise based on the user model. In this paper, we describe a study to evaluate the Adaptive Engine, which compares the benefit (i.e., reduced stress, increased rapport) of tailoring dialogue to the specific user (Adaptive group) with dialogues that are either empathic (Empathic group) or neutral (Neutral group). Results showed a significant reduction in stress in the empathic and neutral groups, but not the adaptive group. Analyses of rule accuracy, participants’ dialogue preferences, and individual differences reveal that the three groups had different needs for empathic dialogue and highlight the importance and challenges of getting the tailoring right.
Synthesis and Technology of Nanoemulsion-Based Pesticide Formulation
Authors: Mustafa Isshadiba Faikah , Hussein Mohd Zobir
Published: August 2020

Declines in crop yield due to pests and diseases require the development of safe, green and eco-friendly pesticide formulations. A major problem faced by the agricultural industry is the use of conventional agrochemicals that contribute broad-spectrum effects towards the environment and organisms. As a result of this issue, researchers are currently developing various pesticide formulations using different nanotechnology approaches. The progress and opportunities in developing nanoemulsions as carriers for plant protection or nanodelivery systems for agrochemicals in agricultural practice have been the subject of intense research. New unique chemical and biologic properties have resulted in a promising pesticide nanoformulations for crop protection. These innovations—particularly the nanoemulsion-based agrochemicals—are capable of enhancing the solubility of active ingredients, improving agrochemical bioavailability, and improving stability and wettability properties during the application, thus resulting in better efficacy for pest control and treatment. All of these—together with various preparation methods towards a greener and environmentally friendly agrochemicals—are also discussed and summarized in this review.
pH-Responsive Nanoparticles for Cancer Immunotherapy: A Brief Review
Authors: Yan Yunfeng , Ding Hangwei
Published: August 2020

Immunotherapy has recently become a promising strategy for the treatment of a wide range of cancers. However, the broad implementation of cancer immunotherapy suffers from inadequate efficacy and toxic side effects. Integrating pH-responsive nanoparticles into immunotherapy is a powerful approach to tackle these challenges because they are able to target the tumor tissues and organelles of antigen-presenting cells (APCs) which have a characteristic acidic microenvironment. The spatiotemporal control of immunotherapeutic drugs using pH-responsive nanoparticles endows cancer immunotherapy with enhanced antitumor immunity and reduced off-tumor immunity. In this review, we first discuss the cancer-immunity circle and how nanoparticles can modulate the key steps in this circle. Then, we highlight the recent advances in cancer immunotherapy with pH-responsive nanoparticles and discuss the perspective for this emerging area.
Cerium Oxide Nanoparticles and Their Efficient Antibacterial Application In Vitro against Gram-Positive and Gram-Negative Pathogens
Authors: Pop Oana L. , Mesaros Amalia , Vodnar Dan C. , Suharoschi Ramona , Tăbăran Flaviu , Magerușan Lidia , Tódor István Sz. , Diaconeasa Zoriţa , Balint Adriana , Ciontea Lelia , Socaciu Carmen
Published: August 2020

In this study, the antibacterial activity of cerium oxide nanoparticles on two Gram-negative and three Gram-positive foodborne pathogens was investigated. CeO2 nanoparticles (CeO2 nps) were synthesized by a Wet Chemical Synthesis route, using the precipitation method and the Simultaneous Addition of reactants (WCS–SimAdd). The as-obtained precursor powders were investigated by thermal analysis (TG–DTA), to study their decomposition process and to understand the CeO2 nps formation. The composition, structure, and morphology of the thermally treated sample were investigated by FTIR, Raman spectroscopy, X-ray diffraction, TEM, and DLS. The cubic structure and average particle size ranging between 5 and 15 nm were evidenced. Optical absorption measurements (UV–Vis) reveal that the band gap of CeO2 is 2.61 eV, which is smaller than the band gap of bulk ceria. The antioxidant effect of CeO2 nps was determined, and the antibacterial test was carried out both in liquid and on solid growth media against five pathogenic microorganisms, namely Escherichia coli, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus. Cerium oxide nanoparticles showed growth inhibition toward all five pathogens tested with notable results. This paper highlights the perspectives for the synthesis of CeO2 nps with controlled structural and morphological characteristics and enhanced antibacterial properties, using a versatile and low-cost chemical solution method.
Pycnogenol® Supplementation Attenuates Memory Deficits and Protects Hippocampal CA1 Pyramidal Neurons via Antioxidative Role in a Gerbil Model of Transient Forebrain Ischemia
Authors: Kim Bora , Lee Tae-Kyeong , Park Cheol Woo , Kim Dae Won , Ahn Ji Hyeon , Sim Hyejin , Lee Jae-Chul , Yang Go Eun , Kim Jong Dai , Shin Myoung Cheol , Cho Jun Hwi , Ryoo Sungwoo , Kim Young-Myeong , Won Moo-Ho , Park Joon Ha
Published: August 2020

Pycnogenol® (an extract of the bark of French maritime pine tree) is used for dietary supplement and known to have excellent antioxidative efficacy. However, there are few reports on neuroprotective effect of Pycnogenol® supplementation and its mechanisms against ischemic injury following transient forebrain ischemia (TFI) in gerbils. Now, we examined neuroprotective effect and its mechanisms of Pycnogenol® in the gerbils with 5-min TFI, which evokes a significant death (loss) of pyramidal cells located in the cornu ammonis (CA1) region of gerbil hippocampus from 4–5 days post-TFI. Gerbils were pretreated with 30, 40, and 50 mg/kg of Pycnogenol® once a day for 7 days before TFI surgery. Treatment with 50 mg/kg, not 30 or 40 mg/kg, of Pycnogenol® potently protected learning and memory, as well as CA1 pyramidal cells, from ischemic injury. Treatment with 50 mg/kg Pycnogenol® significantly enhanced immunoreactivity of antioxidant enzymes (superoxide dismutases and catalase) in the pyramidal cells before and after TFI induction. Furthermore, the treatment significantly reduced the generation of superoxide anion, ribonucleic acid oxidation and lipid peroxidation in the pyramidal cells. Moreover, interestingly, its neuroprotective effect was abolished by administration of sodium azide (a potent inhibitor of SODs and catalase activities). Taken together, current results clearly indicate that Pycnogenol® supplementation can prevent neurons from ischemic stroke through its potent antioxidative role.
Rheological Characteristics of Soluble Fibres during Chemically Simulated Digestion and their Suitability for Gastroparesis Patients
Authors: Suresh Harsha , Ho Vincent , Zhou Jerry
Published: August 2020

Dietary fibres are an integral part of a balanced diet. Consumption of a high-fibre diet confers many physiological and metabolic benefits. However, fibre is generally avoided by individuals with gastrointestinal motility disorders like gastroparesis due to increased likelihood of exacerbated symptoms. Low-viscosity soluble fibres have been identified as a possible source of fibre tolerable for these individuals. The aim of this study is to determine the rheological properties of 10 common commercially available soluble fibres in chemically simulated digestive conditions and evaluate their suitability for individuals with mild to moderate gastroparesis, a gastric motility disorder. Rheological testing under neutral condition (distilled water pH 7) and chemically simulated gastric digestion were evaluated to determine the yield point and relative viscosity of each fibre. Our results reveal two rheological categories of soluble fibres; pseudoplastic and dilatant. Simulated digestion was shown to significantly alter the yield-points of psyllium husk, iota-carrageenan, beta-glucan, apple-fibre pectin, and inulin. Gum Arabic and partially hydrolysed guar gum showed the lowest viscosities and were not affected under simulated digestion, characteristics that make them potential candidate fibres for patients with gastroparesis. Altogether, our results demonstrate that digestion can have a significant impact on fibre viscosity and should be taken into consideration when evaluating the suitability of fibres for patients with gastric motility disorders.
Peptide Release after Simulated Infant In Vitro Digestion of Dry Heated Cow’s Milk Protein and Transport of Potentially Immunoreactive Peptides across the Caco-2 Cell Monolayer
Authors: Zenker Hannah E. , Wichers Harry J. , Tomassen Monic M. M. , Boeren Sjef , De Jong Nicolette W. , Hettinga Kasper A.
Published: August 2020

Dry heating of cow’s milk protein, as applied in the production of “baked milk”, facilitates the resolution of cow’s milk allergy symptoms upon digestion. The heating and glycation-induced changes of the protein structure can affect both digestibility and immunoreactivity. The immunological consequences may be due to changes in the peptide profile of the digested dry heated milk protein. Therefore, cow’s milk protein powder was heated at low temperature (60 °C) and high temperature (130 °C) and applied to simulated infant in vitro digestion. Digestion-derived peptides after 10 min and 60 min in the intestinal phase were measured using LC-MS/MS. Moreover, digests after 10 min intestinal digestion were applied to a Caco-2 cell monolayer. T-cell epitopes were analysed using prediction software, while specific immunoglobin E (sIgE) binding epitopes were identified based on the existing literature. The largest number of sIgE binding epitopes was found in unheated samples, while T-cell epitopes were equally represented in all samples. Transport of glycated peptide indicated a preference for glucosyl lysine and lactosyl-lysine-modified peptides, while transport of peptides containing epitope structures was limited. This showed that the release of immunoreactive peptides can be affected by the applied heating conditions; however, availability of peptides containing epitopes might be limited.
Young Children’s Sugar-Sweetened Beverage Consumption and 5-Year Change in BMI: Lessons Learned from the Timing of Consumption
Authors: Vinke Petra C. , Blijleven Karlien A. , Luitjens Milou H. H. S. , Corpeleijn Eva
Published: August 2020

Sugar-sweetened beverages (SSBs) are an already known risk factor for weight gain in childhood. To identify windows of opportunity for public health interventions, insight into the consumption behavior of SSBs is needed. We investigated whether total SSB consumption was related to body mass index (BMI) change and overweight and compared whether the timing of consumption over the day differed between low and high consumers. In the Dutch GECKO Drenthe birth cohort, a cohort embedded within the Groningen Expert Center for Kids with Obesity (GECKO), height and weight were measured by trained nurses at age 5/6 years (y) and 10/11 y (N = 1257). BMI was standardized for age and sex (BMI-z). In the food pattern questionnaire completed by parents at age 5/6 y, beverages were assessed for seven time segments (breakfast, morning, lunch at school, lunch at home, afternoon, dinner, and evening). Linear and logistic regression analyses were adjusted for potential confounders (sex, baseline BMI-z, parental BMI, parental education level, maternal age at birth, maternal smoking during pregnancy). The median daily SSB consumption frequency ranged from 1.9 times per day (1.5–2.0, 25th–75th percentile) in the lowest quartile to 4.9 times per day (4.6–5.5) in the highest quartile. In the highest compared to the lowest quartile of SSB consumption frequency, the confounder-adjusted odds ratio for overweight incidence was 3.12 (95% CI, 1.60–6.07). The difference in consumption between quartile 1 and quartile 4 occurred mainly during main meals and in the evening, e.g., at breakfast (31% vs. 98%, p < 0.001), lunch at home (32% vs. 98%, p < 0.001), and dinner (17% vs. 72%, p < 0.001). These drinking occasions characterizing high SSB consumers mostly occurred in the home environment, where parental influence on dietary behaviors is profound. Therefore, these results exposed a window of opportunity, leading to the advice for parents to offer their children sugar-free drinks to quench thirst with main meals.
Molecular Epidemiology Reveals Low Genetic Diversity among Cryptococcus neoformans Isolates from People Living with HIV in Lima, Peru, during the Pre-HAART Era
Authors: van de Wiele Nathalie , Neyra Edgar , Firacative Carolina , Gilgado Felix , Serena Carolina , Bustamante Beatriz , Meyer Wieland
Published: August 2020

Cryptococcosis, a mycosis presenting mostly as meningoencephalitis, affecting predominantly human immunodeficiency virus (HIV)-infected people, is mainly caused by Cryptococcus neoformans. The genetic variation of 48 C. neoformans isolates, recovered from 20 HIV-positive people in Lima, Peru, during the pre-highly active antiretroviral therapy (HAART) era, was studied retrospectively. The mating type of the isolates was determined by PCR, and the serotype by agglutination and CAP59-restriction fragment length polymorphism (RFLP). Genetic diversity was assessed by URA5-RFLP, PCR-fingerprinting, amplified fragment length polymorphism (AFLP), and multilocus sequence typing (MLST). All isolates were mating type alpha, with 39 molecular type VNI, seven VNII, corresponding to C. neoformans var. grubii serotype A, and two VNIII AD hybrids. Overall, the cryptococcal population from HIV-positive people in Lima shows a low degree of genetic diversity. In most patients with persistent cryptococcal infection, the same genotype was recovered during the follow-up. In four patients with relapse and one with therapy failure, different genotypes were found in isolates from the re-infection and from the isolate recovered at the end of the treatment. In one patient, two genotypes were found in the first cryptococcosis episode. This study contributes data from Peru to the ongoing worldwide population genetic analysis of Cryptococcus.
Water as a Source of Antimicrobial Resistance and Healthcare-Associated Infections
Authors: Hayward Claire , Ross Kirstin E. , Brown Melissa H. , Whiley Harriet
Published: August 2020

Healthcare-associated infections (HAIs) are one of the most common patient complications, affecting 7% of patients in developed countries each year. The rise of antimicrobial resistant (AMR) bacteria has been identified as one of the biggest global health challenges, resulting in an estimated 23,000 deaths in the US annually. Environmental reservoirs for AMR bacteria such as bed rails, light switches and doorknobs have been identified in the past and addressed with infection prevention guidelines. However, water and water-related devices are often overlooked as potential sources of HAI outbreaks. This systematic review examines the role of water and water-related devices in the transmission of AMR bacteria responsible for HAIs, discussing common waterborne devices, pathogens, and surveillance strategies. AMR strains of previously described waterborne pathogens including Pseudomonas aeruginosa, Mycobacterium spp., and Legionella spp. were commonly isolated. However, methicillin-resistant Staphylococcus aureus and carbapenem-resistant Enterobacteriaceae that are not typically associated with water were also isolated. Biofilms were identified as a hot spot for the dissemination of genes responsible for survival functions. A limitation identified was a lack of consistency between environmental screening scope, isolation methodology, and antimicrobial resistance characterization. Broad universal environmental surveillance guidelines must be developed and adopted to monitor AMR pathogens, allowing prediction of future threats before waterborne infection outbreaks occur.
Total Synthesis of Natural Disaccharide Sambubiose
Authors: Lucarini Simone , Ciulla Maria Gessica , Mestichelli Paola , Duranti Andrea
Published: August 2020

A practical and robust synthetic method to obtain the natural disaccharide sambubiose (2-O-β-D-xylopyranosyl-D-glucopyranose) is reported, exploring the key step in the synthesis, i.e., stereoselective O-glycosylation. Specifically, the best combinations of glycoside donors and acceptors were identified, stereospecific control of the reaction was achieved by screening several catalysts and protection/deprotection steps were evaluated and improved. The best result was obtained by coupling allyl 3,5,6-tri-O-benzyl-β-D-glucofuranoside with 2,3,4-tri-O-acetyl-D-xylopiranosyl-α-trichloro acetimidate in the presence of trimethylsilyl triflate as a catalyst giving the corresponding protected target compound as a correct single isomer. The latter was transformed accordingly into the desired final product by deprotection steps (deallylation, deacetylation, and debenzylation). Sambubiose was synthesized into a satisfactory and higher overall yield than previously reported and was also characterized.
A Systematic Review of Molecular Imaging Agents Targeting Bradykinin B1 and B2 Receptors
Authors: Lau Joseph , Rousseau Julie , Kwon Daniel , Bénard François , Lin Kuo-Shyan
Published: August 2020

Kinins, bradykinin and kallidin are vasoactive peptides that signal through the bradykinin B1 and B2 receptors (B1R and B2R). B2R is constitutively expressed in healthy tissues and mediates responses such as vasodilation, fluid balance and retention, smooth muscle contraction, and algesia, while B1R is absent in normal tissues and is induced by tissue trauma or inflammation. B2R is activated by kinins, while B1R is activated by kinins that lack the C-terminal arginine residue. Perturbations of the kinin system have been implicated in inflammation, chronic pain, vasculopathy, neuropathy, obesity, diabetes, and cancer. In general, excess activation and signaling of the kinin system lead to a pro-inflammatory state. Depending on the disease context, agonism or antagonism of the bradykinin receptors have been considered as therapeutic options. In this review, we summarize molecular imaging agents targeting these G protein-coupled receptors, including optical and radioactive probes that have been used to interrogate B1R/B2R expression at the cellular and anatomical levels, respectively. Several of these preclinical agents, described herein, have the potential to guide therapeutic interventions for these receptors.
The Application of Inosine 5′-Monophosphate Dehydrogenase Activity Determination in Peripheral Blood Mononuclear Cells for Monitoring Mycophenolate Mofetil Therapy in Children with Nephrotic Syndrome
Authors: Sobiak Joanna , Jóźwiak Alicja , Wziętek Honorata , Zachwieja Jacek , Ostalska-Nowicka Danuta
Published: August 2020

In pediatric nephrotic syndrome, recommended mycophenolic acid (MPA) pharmacokinetics are higher than those for transplant recipients. In MPA therapeutic monitoring, inosine-5′-monophosphate dehydrogenase (IMPDH) activity may be useful. We modified the method established for renal transplant recipients and determined IMPDH activity in peripheral blood mononuclear cells (PBMCs) from healthy volunteers and children (4–16 years) with nephrotic syndrome treated with mycophenolate mofetil (MMF). From children, four blood samples were collected, and MPA concentrations were also determined. IMPDH activity was calculated using xanthosine monophosphate (XMP) normalized with adenosine monophosphate (AMP), both determined with the HPLC-UV method. The modified method was accurate, precise, and linear for AMP and XMP within 0.50–50.0 μmoL/L. Mean IMPDH activity in volunteers was 45.97 ± 6.24 µmoL·s−1·moL−1 AMP, whereas for children, the values were variable and amounted to 39.23 ± 27.40 µmoL·s−1·moL−1 AMP and 17.97 ± 15.24 µmoL·s−1·moL−1 AMP before the next MMF dose and 1 h afterward, respectively. The modified method may be applied to IMPDH activity determination in children with nephrotic syndrome treated with MMF. IMPDH activity should be determined after one thawing of PBMCs due to the change in AMP and XMP concentrations after subsequent thawing. For children, the lowest IMPDH activity was observed concomitantly with the highest MPA concentration.
Stability of Ophthalmic Atropine Solutions for Child Myopia Control
Authors: Berton Baptiste , Chennell Philip , Yessaad Mouloud , Bouattour Yassine , Jouannet Mireille , Wasiak Mathieu , Sautou Valérie
Published: August 2020

Myopia is an ophthalmic condition affecting more than 1/5th of the world population, especially children. Low-dose atropine eyedrops have been shown to limit myopia evolution during treatment. However, there are currently no commercial industrial forms available and there is little data published concerning the stability of medications prepared by compounding pharmacies. The objective of this study was to evaluate the stability of two 0.1 mg/mL atropine formulations (with and without antimicrobiobial preservatives) for 6 months in two different low-density polyethylene (LDPE) multidose eyedroppers. Analyses used were the following: visual inspection, turbidity, chromaticity measurements, osmolality and pH measurements, atropine quantification by a stability-indicating liquid chromatography method, breakdown product research, and sterility assay. In an in-use study, atropine quantification was also performed on the drops emitted from the multidose eyedroppers. All tested parameters remained stable during the 6 months period, with atropine concentrations above 94.7% of initial concentration. A breakdown product (tropic acid) did increase slowly over time but remained well below usually admitted concentrations. Atropine concentrations remained stable during the in-use study. Both formulations of 0.1 mg/mL of atropine (with and without antimicrobial preservative) were proved to be physicochemically stable for 6 months at 25 °C when stored in LDPE bottles, with an identical microbial shelf-life.
The Consequences of General Medication Beliefs Measured by the Beliefs about Medicine Questionnaire on Medication Adherence: A Systematic Review
Authors: Shahin Wejdan , Kennedy Gerard A. , Stupans Ieva
Published: August 2020

(1) Background: Medication adherence is a key determinant of patient health outcomes in chronic illnesses. However, adherence to long-term therapy remains poor. General beliefs about medicine are considered factors influencing medication adherence. It is essential to address the gap in the literature regarding understanding the impact of general beliefs about medicine on medication adherence to promote adherence in chronic illnesses. (2) Methods: PubMed, CINHAL, and EMBASE databases were searched. Studies were included if they examined medication beliefs using the Beliefs about Medicine Questionnaire in one of four chronic illnesses: hypertension, diabetes, chronic obstructive pulmonary disease, and/or asthma. (3) Results: From 1799 articles obtained by the search, only 11 met the inclusion criteria. Hypertension and diabetes represented 91% of included studies, while asthma represented 9%. Higher medication adherence was associated with negative general medication beliefs; 65% of the included studies found a negative association between harm beliefs and adherence, while 30% of studies found a negative association with overuse beliefs. (4) Conclusions: This review evaluated the impact of harm and overuse beliefs about medicines on medication adherence, highlighting the gap in literature regarding the impact of harm and overuse beliefs on adherence. Further research is needed to fully identify the association between general beliefs and medication adherence in people with different cultural backgrounds, and to explore these beliefs in patients diagnosed with chronic illnesses, particularly asthma and chronic obstructive pulmonary disease (COPD). Healthcare providers need to be aware of the impact of patients’ cultural backgrounds on general medication beliefs and adherence.
Constituents of Gastrodia elata and Their Neuroprotective Effects in HT22 Hippocampal Neuronal, R28 Retinal Cells, and BV2 Microglial Cells
Authors: Kim Hye Mi , Kwon Jaeyoung , Lee Kyerim , Lee Jae Wook , Jang Dae Sik , Kwon Hak Cheol
Published: August 2020

Gastrodia elata is widely used in traditional medicine and contains various types of metabolites with pharmacological activity. In the course of searching for neuroprotective molecules associated with the potential of G. elata in the treatment of neurodegenerative disorders, two new phenolic compounds (1 and 2) and a new tripeptide (3), together with 16 known compounds (4–19), were isolated from the rhizomes of G. elata. The structures of the compounds were determined by the interpretation of spectroscopic data, including nuclear magnetic resonance and mass spectrometry data. All obtained compounds were assessed for their ability to protect neuronal cells against neurotoxicity and neuroinflammation. Of these, 4 and 5 were found to possess moderate activities in HT22 hippocampal neuronal cells, whereas 2, 6, and 7 showed weak activities in R28 retinal cells. Additionally, compound 9 showed moderate inhibitory activity on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells.
Influence Mechanism and Optimization Analysis of Technological Parameters for the Composite Prepreg Tape Winding Process
Authors: Deng Bo , Shi Yaoyao , Yu Tao , Zhao Pan
Published: August 2020

Composite prepreg tape winding technology has proven to be an effective method for manufacturing revolving body composite structures in aerospace field. Process parameters including heating temperature, tape tension and roller pressure have an important impact on the winding products’ mechanical property such as tensile strength. The aim of this study is to investigate the influence mechanism and optimization analysis of parameters for the composite prepreg tape winding process. Firstly, the sensitivity analysis for single parameter had be employed to reveal the influence mechanism of each winding parameter change on tensile strength. Secondly, iso-surfaces analysis for parameter range had be applied to describe the distribution law of parameter with continuous distribution characteristics. Then the coupling analysis for process parameters was carried out employing response surface methodology. The analysis results showed that tape tension has the most significant effect on the winding products’ tensile strength. And the outstanding parameter combination with the heating temperature of 72 °C, tape tension of 307 N and roller pressure of 1263 N was provided by response surface design software via desirability function method. The validation experiments showed that the optimal parameter combination has a positive guiding significance for improving the quality of winding products.
State of the Art on Biomaterials for Soft Tissue Augmentation in the Oral Cavity. Part II: Synthetic Polymers-Based Biomaterials
Authors: Toledano Manuel , Toledano-Osorio Manuel , Carrasco-Carmona Álvaro , Vallecillo Cristina , Toledano Raquel , Medina-Castillo Antonio Luis , Osorio Raquel
Published: August 2020

Most of the polymers used as biomaterials for scaffolds are naturally occurring, synthetic biodegradable, and synthetic non-biodegradable polymers. Since synthetic polymers can be adapted for obtaining singular desired characteristics by applying various fabrication techniques, their use has increased in the biomedical field, in dentistry in particular. The manufacturing methods of these new structures include many processes, such as electrospinning, 3D printing, or the use of computer-aided design/computer-aided manufacturing (CAD/CAM). Synthetic polymers show several drawbacks that can limit their use in clinical applications, such as the lack of cellular recognition, biodegradability, and biocompatibility. Moreover, concerning biodegradable polymers, the time for matrix resorption is not predictable, and non-resorbable matrices are preferred for soft tissue augmentation in the oral cavity. This review aimed to determine a new biomaterial to offset the present shortcomings in the oral environment. Researchers have recently proposed a novel non-resorbable composite membrane manufactured via electrospinning that has allowed obtaining remarkable in vivo outcomes concerning angiogenesis and immunomodulation throughout the polarization of macrophages. A prototype of the protocol for in vitro and in vivo experimentation with hydrogels is explained in order to encourage innovation into the development of promising biomaterials for soft tissue augmentation in the near future.
Preparation and Electrochemical Properties of Porous Carbon Nanofiber Electrodes Derived from New Precursor Polymer: 6FDA-TFMB
Authors: Jeon Byeongil , Ha Taehwa , Lee Dong Yun , Choi Myung-Seok , Lee Seung Woo , Jung Kyung-Hye
Published: August 2020

Porous carbon nanofibers (CNFs) with high energy storage performance were fabricated with a single precursor polymer, 6FDA-TFMB, without the use of any pore-generating materials. 6FDA-TFMB was synthesized, electrospun, and thermally treated to produce binder-free CNF electrodes for electrochemical double-layer capacitors (EDLCs). Highly porous CNFs with a surface area of 2213 m2 g−1 were prepared by steam-activation. CNFs derived from 6FDA-TFMB showed rectangular cyclic voltammograms with a specific capacitance of 292.3 F g−1 at 10 mV s−1. It was also seen that CNFs exhibit a maximum energy density of 13.1 Wh kg−1 at 0.5 A g−1 and power density of 1.7 kW kg−1 at 5 A g−1, which is significantly higher than those from the common precursor polymer, polyacrylonitrile (PAN).
In Vitro Degradation of Electrospun Poly(Lactic-Co-Glycolic Acid) (PLGA) for Oral Mucosa Regeneration
Authors: Chor Ana , Gonçalves Raquel Pires , Costa Andrea Machado , Farina Marcos , Ponche Arnaud , Sirelli Lys , Schrodj Gautier , Gree Simon , Andrade Leonardo Rodrigues de , Anselme Karine , Dias Marcos Lopes
Published: August 2020

Poly(lactic-co-glycolic acid) (PLGA) has been used in the field of tissue engineering as a scaffold due to its good biocompatibility, biodegradability and mechanical strength. With the aim to explore the degradability of PLGA electrospun nonwoven structures for oral mucosa tissue engineering applications, non-irradiated and gamma irradiated nonwovens were immersed in three different solutions, in which simulated body fluid (SBF) and artificial saliva are important for future oral mucosa tissue engineering. The nonwovens were immersed for 7, 15 and 30 days in SBF, culture media (DMEM) and artificial saliva at 37 °C. Before immersion in the solutions, the dosage of 15 kGy was applied for sterilization in one assay and compared with non-irradiated samples at the same timepoints. Samples were characterized using different techniques such as scanning electron microscopy (SEM), differential scanning calorimetric (DSC) and gel permeation chromatography (GPC) to evaluate the nonwoven degradation and Fourier-transform infrared spectroscopy (FTIR) to evaluate the chain scissions. Our results showed that PLGA nonwovens were constituted by semicrystalline fibers with moderate degradation properties up to thirty days. The non-irradiated samples exhibited slower kinetics of degradation than irradiated nonwovens. For immersion times longer than 7 days in the three different solutions, the mean diameter of irradiated fibers stayed in the same range, but significantly different from the control sample. On non-irradiated samples, the degradation kinetics was slower and the plateau in the diameter value was only attained after 30 days of immersion in the fluids. Plasticization (fluid absorption into the fiber structure) occurred in the bulk material, as confirmed by a decrease in Tg observed by DSC analyses of non-irradiated and irradiated nonwovens, in comparison with the respective controls. In addition, artificial saliva showed a higher capacity of influencing PLGA crystallization than SBF and DMEM. FTIR analyses showed typical PLGA chemical functional groups changes. These results will be important for future application of those PLGA electrospun nonwovens for oral mucosa regeneration.
Efficient Removal of Ni(II) from Aqueous Solution by Date Seeds Powder Biosorbent: Adsorption Kinetics, Isotherm and Thermodynamics
Authors: Elkhaleefa Abubakr , Ali Ismat H. , Brima Eid I. , Elhag A. B. , Karama Babiker
Published: August 2020

Adsorption investigations in batch approaches were performed to explore the biosorption of Ni(II) ions from aqueous solutions on date seeds powder. The effects of pH, particle size, initial concentration of Ni(II) ions, adsorbent mass, temperature, and contact on the adsorption efficacy were studied. The maximum removal obtained was 90% for an original Ni(II) ion solution concentration of 50 ppm was attained at pH 7 after 30 min and with 0.30 g of an added adsorbent. The four adsorption models, namely Freundlich, Langmuir, Dubinin–Radushkevich (D–R), and Temkin were examined to fit the experimental findings. The adsorption system obeys the Freundlich model. The system was found to follow the pseudo-second order kinetic model. Thermodynamic factors; entropy (ΔS°), enthalpy (ΔH°), and Gibbs free energy (ΔG°) changes were also assessed. Results proved that adsorption of Ni(II) ions is exothermic and spontaneous. Sticking probability value was found to be less than unity, concluding that the process is dominated by physical adsorption.
Plasma Agriculture from Laboratory to Farm: A Review
Authors: Attri Pankaj , Ishikawa Kenji , Okumura Takamasa , Koga Kazunori , Shiratani Masaharu
Published: August 2020

In recent years, non-thermal plasma (NTP) application in agriculture is rapidly increasing. Many published articles and reviews in the literature are focus on the post-harvest use of plasma in agriculture. However, the pre-harvest application of plasma still in its early stage. Therefore, in this review, we covered the effect of NTP and plasma-treated water (PTW) on seed germination and growth enhancement. Further, we will discuss the change in biochemical analysis, e.g., the variation in phytohormones, phytochemicals, and antioxidant levels of seeds after treatment with NTP and PTW. Lastly, we will address the possibility of using plasma in the actual agriculture field and prospects of this technology.
Processes for Bioenergy and Resources Recovery from Biowaste
Authors: Elbeshbishy Elsayed , Dhar Bipro Ranjan
Published: August 2020

The increasing quantity of biowaste generation and the requirements governing their ultimate disposal are of serious economic and environmental concern [...]
Remote Sensing of Agricultural Greenhouses and Plastic-Mulched Farmland: An Analysis of Worldwide Research
Authors: Jiménez-Lao Rafael , Aguilar Fernando J. , Nemmaoui Abderrahim , Aguilar Manuel A.
Published: August 2020

The total area of plastic-covered crops of 3019 million hectares has been increasing steadily around the world, particularly in the form of crops maintained under plastic-covered greenhouses to control their environmental conditions and their growth, thereby increasing production. This work analyzes the worldwide research dynamics on remote sensing-based mapping of agricultural greenhouses and plastic-mulched crops throughout the 21st century. In this way, a bibliometric analysis was carried out on a total of 107 publications based on the Scopus database. Different aspects of these publications were studied, such as type of publication, characteristics, categories and journal/conference name, countries, authors, and keywords. The results showed that “articles” were the type of document mostly found, while the number of published documents has exponentially increased over the last four years, growing from only one document published in 2001 to 22 in 2019. The main Scopus categories relating to the topic analyzed were Earth and Planetary Sciences (53%), Computer Science (30%), and Agricultural and Biological Sciences (28%). The most productive journal in this field was “Remote Sensing”, with 22 documents published, while China, Italy, Spain, USA, and Turkey were the five countries with the most publications. Among the main research institutions belonging to these five most productive countries, there were eight institutions from China, four from Italy, one from Spain, two from Turkey, and one from the USA. In conclusion, the evolution of the number of publications on Remote Sensing of Agricultural Greenhouses and Plastic-Mulched Farmland found throughout the period 2000–2019 allows us to classify the subject studied as an emerging research topic that is attracting an increasing level of interest worldwide, although its relative significance is still very limited within the remote sensing discipline. However, the growing demand for information on the arrangement and spatio-temporal dynamics of this increasingly important model of intensive agriculture is likely to drive this line of research in the coming years.
Dynamics and Drivers of the Alpine Timberline on Gongga Mountain of Tibetan Plateau-Adopted from the Otsu Method on Google Earth Engine
Authors: He Wen , Ye Chongchong , Sun Jian , Xiong Junnan , Wang Jinniu , Zhou Tiancai
Published: August 2020

The alpine timberline, an ecosystem ecotone, indicates climatic change and is tending to shift toward higher altitudes because of an increase in global warming. However, spatiotemporal variations of the alpine timberline are not consistent on a global scale. The abundant and highest alpine timberline, located on the Tibetan Plateau, is less subject to human activity and disturbance. Although many studies have investigated the alpine timberline on the Tibetan Plateau, large-scale monitoring of spatial-temporal dynamics and driving mechanisms of the alpine timberline remain uncertain and inaccurate. Hence, the Gongga Mountain on the southeastern Tibetan Plateau was chosen as the study area because of the most complete natural altitudinal zonation. We used the Otsu method on Google Earth Engine to extract the alpine timberline from 1987–2019 based on the normalized difference vegetation index (NDVI). Then, the alpine timberline spatiotemporal patterns and the effect of topography on alpine timberline distribution were explored. Four hillsides on the western Gongga Mountain were selected to examine the hillside differences and drivers of the alpine timberline based on principal component analysis (PCA) and multiple linear regression (MLR). The results indicated that the elevation range of alpine timberline was 3203–4889 m, and the vegetation coverage increased significantly (p < 0.01) near the alpine timberline ecotone on Gongga Mountain. Moreover, there was spatial heterogeneity in dynamics of alpine timberline, and some regions showed no regular trend in variations. The spatial pattern of the alpine timberline was generally high in the west, low in the east, and primarily distributed on 15–55° slopes. Besides, the drivers of the alpine timberline have the hillside differences, and the sunny and shady slopes possessed different driving factors. Thus, our results highlight the effects of topography and climate on the alpine timberline on different hillsides. These findings could provide a better approach to study the dynamics and formation of alpine timberlines.