bluuebunny/crossref_metadata_2025 huggingface dataset:
miR-3156-5p Enhances Hypoxia-Induced Angiogenesis in Hepatocellular Carcinoma via Modulating the SOCS5/HIF-1α/VEGF Pathway
Authors: Tie Bin , Guo Zheng , Li Li , Wang Wenhui , Liu Rong , Fang Jin
Published: September 2021

Background: MicroRNAs (miRNAs) are dysregulated in hypoxia-induced hepatocellular carcinoma (HCC). This study probed the regulatory mechanism of miR-3156-5p on HCC under hypoxia. Methods: HCC cells (HepG2) were exposed to normoxia or hypoxia, and the conditioned medium (CM) of HepG2 was applied. Quantitative reverse transcription PCR (qRT-PCR) was implemented to analyze the miR-3156-5p profile. The cell counting kit-8 (CCK-8) assay and the colony formation experiment were conducted to measure cell proliferation, colony formation, and angiogenesis. Results: The results manifested that miR-3156-5p was up-regulated in HCC cells and endothelial cells under hypoxia, and up-regulating miR-3156-5p boosted HCC cell proliferation, endothelial cell angiogenesis, and HIF-1α/VEGF expression. Conclusions: miR-3156-5p activates the HIF-1α/VEGF pathway by hampering SOCS5, thereby enhancing the angiogenic potential of hypoxia-induced endothelial cells in HCC cells.
Niacin mitigates rumen epithelial damage in vivo by inhibiting rumen epithelial cell apoptosis on a high concentrate diet
Authors: Gao Zhen , Li Yanjiao , Xu Chao , Luo Dan , Qiu Qinghua , Pan Ke , Xiong Xiaowen , Qu Mingren , Ouyang Kehui
Published: September 2021

To investigate the effects of niacin on rumen fermentation, rumen epithelial antioxidant activity, and rumen epithelial cell apoptosis on high concentrate (HC) diets, nine male Hu sheep were randomly divided into: low concentrate diet (LC; concentrate : forage (C:F) = 20:80, high concentrate diet (HC; C:F = 80:20), and HCN diet (HC diet + niacin at 800 mg/kg diet air-dry matter). Compared with the LC group, the HC group had a lower rumen pH, increased volatile fatty acids and lactic acid in the rumen, reduced activity of antioxidant enzymes and total antioxidant capacity, and increased malondialdehyde content in the rumen epithelium (P < 0.05). Rumen epithelial papilla morphology was decreased, and apoptosis-related indicators and serum inflammatory cytokines were increased in the HC group over the LC group (P < 0.05). Compared with the HC diet, the HCN diet increased rumen pH, rumen epithelium antioxidant capacity, and rumen epithelial papilla morphology, decreased rumen lactate content, serum inflammatory cytokines, and apoptosis-related indicators (P < 0.05). Therefore, adding 800 mg/kg niacin helped protect against rumen epithelial damage by avoiding drastic changes in the rumen environment and improved rumen epithelial antioxidant capacity to inhibit rumen epithelial cell apoptosis in sheep on a HC diet.
Morphological and molecular analyses of Taenia and Mesocestoides species from red foxes (Vulpes vulpes) in northwestern China
Authors: Liu Gang , Ji Na , Hornok Sándor , Zhang Yu , Zhao Shanshan , Chen Xueling , Hazihan Wurelihazi , Gu Xinli , Wang Yuan-Zhi
Published: September 2021

Background
The red fox (Vulpes vulpes) is a geographically widespread predator species, occurring across the northern Hemisphere. It has high veterinary-medical significance, in part owing to its definitive host role in the life cycle of several tapeworm species, including zoonotic ones. However, there have been only few reports on cyclophyllid tapeworm (Cestoda: Cyclophyllidea) infection in red foxes from China.
Methods
In this study, 263 tapeworms were collected from eight road-killed red foxes in Xinjiang Uygur Autonomous Region (XUAR, northwestern China). The tapeworms were analyzed based on morphological characters and mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences.
Results
83 Taenia and 180 Mesocestoides tapeworms were identified according to the presence or absence of rostellum, and the number, the length and the shape of the large rostellar hooks. The morphological and molecular analyses revealed that i) 180 Mesocestoides tapeworms, here named as Mesocestoides sp. Vulpes vulpes, showed 99.21% (378/381 bp) identity to Mesocestoides sp. reported from red fox in Mongolia; and ii) 83 Taenia tapeworms belonged to three species. The first Taenia species (n = 16, named as Taenia sp. Vulpes vulpes), based on the length of large rostellar hooks (337–342 µm) and its cox1 sequence, was identified as a potentially novel species, which is phylogenetically close to Taenia laticollis. The second species (n = 54, named as Taenia sp. Vulpes vulpes & Rhombomys opimus), was morphologically similar to Taenia endothoracicus according to the number (n = 52), the length (319–332 µm) and the shape of the large rostellar hooks. This species, infecting three red foxes, shared 100% cox1 sequence identity with Taenia sp. Rhombomys opimus genotype C found previously in great gerbils (Rhombomys opimus) in the same region. The third species (n = 13, named as Taenia polyacantha-like), had shorter large rostellar hooks (178–180 µm) and showed 96.27% (361/375 bp) sequence identity to Taenia polyacantha reported from red fox in Italy. In addition, co-infections with two Taenia species and with Taenia and Mesocestoides species were also demonstrated.
Conclusions
The “great gerbil-red fox” life cycle of Taenia sp. Vulpes vulpes & Rhombomys opimus, belonging to the mitochondrial lineage of T. endothoracicus, is confirmed. The T. polyacantha-like species and Mesocestoides sp. Vulpes vulpes were found for the first time in red fox in China. Taenia sp. Vulpes vulpes is a potentially novel species, which is close to Taenia laticollis based on its phylogenetic properties. The life cycle of Taenia sp. Vulpes vulpes and Mesocestoides sp. Vulpes vulpes should be further explored.
ICU-Acquired Pneumonia is a Risk Factor of a Poor Health Post-Covid-19 Syndrome
Authors: Martin-Loeches Ignacio , Motos Anna , Menéndez Rosario , Gabarrus Albert , González Jessica , Fernández-Barat Laia , Ceccato Adrián , Pérez Raquel , García-Gasulla Dario , Ferrer Ricard , Riera Jordi , Lorente José Ángel , Peñuelas Oscar , Bermejo-Martin Jesús F , de Gonzalo-Calvo David , Rodríguez Alejandro , Barbé Ferran , Aguilera Luciano , Villar Rosario Amaya , Barberà Carme , Barberán José , Ortiz Aaron Blandino , Bustamante-Munguira Elena , Caballero Jesús , Carbajales Cristina , Carbonell Nieves , Catalán-González Mercedes , Galbán Cristóbal , Sanguino Víctor D. Gumucio , Torre Maria del Carmen de la , Díaz Emili , Gallego Elena , Garmendia José Luis García , Garnacho-Montero José , Gómez José M. , García Ruth Noemí Jorge , Loza-Vázquez Ana , Marin-Corral Judith , Gándara Amalia Martínez de la , Varela Ignacio Martínez , Messa Juan Lopez , Albaiceta Guillermo M , Novo Mariana Andrea , Peñasco Yhivian , Ricart Pilar , Urrelo-Cerrón Luis , Sánchez-Miralles Angel , Chinesta Susana Sancho , Socias Lorenzo , Solé-Violan Jordi , Lomas Luis Tamayo , Vidal Pablo , Torres Antoni
Published: September 2021

Background. Some patients who had previously presented with COVID-19 have been reported to develop persistent COVID-19 symptoms. Whilst this information has been adequately recognised and extensively published with respect to non-critically ill patients, less is known about the prevalence and risk factors and characteristics of persistent COVID_19 . On other hand these patients have very often intensive care unit-acquired pneumonia (ICUAP). A second infectious hit after COVID increases the length of ICU stay and mechanical ventilation and could have an influence in the poor health post-Covid 19 syndrome in ICU discharged patientsMethods: This prospective, multicentre and observational study was done across 40 selected ICUs in Spain. Consecutive patients with COVID-19 requiring ICU admission were recruited and evaluated three months after hospital discharge. Results: A total of 1,255 ICU patients were scheduled to be followed up at 3 months; however, the final cohort comprised 991 (78.9%) patients. A total of 315 patients developed ICUAP (97% of them had ventilated ICUAP) Patients requiring invasive mechanical ventilation had persistent, post-COVID-19 symptoms than those who did not require mechanical ventilation. Female sex, duration of ICU stay, and development of ICUAP were independent risk factors for persistent poor health post-COVID-19.Conclusions: Persistent, post-COVID-19 symptoms occurred in more than two-thirds of patients. Female sex, duration of ICU stay and the onset of ICUAP comprised all independent risk factors for persistent poor health post-COVID-19. Prevention of ICUAP could have beneficial effects in poor health post-Covid 19
Deposit Determinants and Domestic Currency Deposits In Nigeria (2000-2018)
Authors: Abiodun Ayodeji Emmanuel , Ogundipe Adebayo Tunbosun , Musa-Agboneni Omomen
Published: September 2021

The study was carried out to investigate the effects of macroeconomic and banking sector-specific variables on domestic currency deposits in Nigeria within a temporal scope 2000-2018. On the theoretical threshold of Ayodeji-Ajala bank-intermediation (economic value) theorem, the study proxied the dependent variable, domestic currency deposit, by total domestic currency deposit of banks, and the independent variable, deposit determinants, by three macroeconomic variables (interest rate, gross domestic product, inflation) and two banking sector-specific variables (private sector credit and bank size). Secondary data were sourced from the Central Bank of Nigeria statistical bulletin of various editions, and were estimated using Auto Regressive Distributed Lag (ARDL) approach. It was found that, while interest rates exhibited insignificant negative effects on domestic currency deposits in Nigeria, inflation rate exerted significant negative effect on it, and gross domestic product exerted significant positive effect on it. It was, also, found that, private sector credit exerted a significant positive effect on domestic currency deposit while bank size exhibited insignificant positive effect on it. It was, further, found that, a significant long-run relationship existed between deposit determinants and domestic currency deposits in Nigeria. It was, therefore, concluded that macroeconomic and banking sector-specific variables exert significant long-term influence on domestic currency deposits in Nigeria. It was, therefore, recommended that, government should effectively adopt the instruments of monetary and fiscal policies for enhancing the effects of interest rates and curbing the effects of inflation on the Nigerian economy. Also, banks are encouraged to invest more on their assets, as this would help attract more customers of various types while they should increase private sector credits, and channel the same to the productive sector of the economy so that economic growth can be enhanced.
Fluorescence recognition of Zn2+ ion by 1,1′-binaphthyl based on polyamine macrocycle
Authors: Tokunaga Hiroki , Tsuboi Masaki , Matsumoto Nachika , Miyasaka Makoto
Published: September 2021

Chiral polyimine macrocycles were synthesized by the self-condensation of (R)-2,2'-dihydroxy-1,1'-binaphthalene-3,3'-dicarbaldehyde and o-phenylenediamine derivatives under template-free conditions in relatively high yields, and the corresponding polyamine macrocycles were obtained by the reduction of the polyimine macrocycles. The complexation behaviors of the polyamine macrocycles with different metal cations in ethanol were studied based on their UV-vis absorption spectra and fluorescence spectra. No fluorescence was observed for the polyimine macrocycles (R,R)-1–(R,R)-3, however, (R,R)-1H and (R,R)-2H exhibited a “turn-on” blue fluorescence (λem = 415 nm) that were observed upon the addition of Zn2+. Especially, (R,R)-3H complexed with Zn2+ also produced a “turn-on” yellowish green fluorescence (λem = 510 nm).
Generation of Inverted Locomotion Gait for Multi-Legged Robots Using a Spherical Magnet Joint and Adjustable Sleeve
Authors: Sison Harn , Ratsamee Photchara , Higashida Manabu , Uranashi Yuki , Haruo Takemura
Published: September 2021

In this paper, we propose a design and an implementation of spherical magnet joint (SMJ) - based gait generation for inverted locomotion of multi-legged robots. A spherical permanent magnet is selected to generate a consistent attractive force for the robot to perform inverted locomotion under steel structures. Additionally, the tip of the robot's foot is designed as a ball-joint mechanism to give flexibility to the foot placement at any angle between the tip and surfaces. We also propose an adjustable sleeve mechanism to detach the tip of the foot during locomotion by creating a fulcrum point during the tilt and pull step. As a result, the reaction force can be reduced according to sleeve diameter. Experimental results show that the presented load decreased by 46% from direct pulling with the adjustable sleeve mechanism. For inverted locomotion, a quadruped robot and a hexapod robot were constructed to represent the predominant type of multi-legged robot. We integrated the SMJ and the adjustable sleeve on both robots and performed the inverted locomotion with a crawling gait, a trotting gait, a square gait, and a tripod gait. Our analysis demonstrates the characteristics of each gait in terms of velocity, stability, guaranteeing the versatility of our proposed SMJ, which can be applied to different types of legged robots.
Lipid Raft Microdomains of Bladder Epithelial Cells Regulates Apoptosis and Microenvironment Upon Exposure to C. albicans
Authors: Kim Jayoung , Yeon Austin , Ahmed Khandakar , Zhang Wei , Kim Khae-Hawn , Kim Woong-Ki
Published: September 2021

INTRODUCTION. Interstitial cystitis/painful bladder syndrome (IC) is characterized by chronic bladder pain and urinary storage symptoms. IC affects more than 3.3 million women in the U.S. alone. Ibis T-5000 assays and next generation sequencing have revealed that the C. albicans fungus is highly abundant in the urine of IC patients, particularly those who report greater pain, urinary urgency, and flares. However, currently, the clinical significance of C. albicans in the urine remains elusive. Here, we report the pathological effects and mechanisms triggered by C. albicans in a healthy normal bladder.
METHODS. Immortalized bladder epithelial cells were infected with C. albicans. Perturbations in gene expression were identified using an Affymetrix gene microarray and subsequently followed with bioinformatic analyses, including gene set enrichment. Inflammatory and apoptotic genes were quantified using RT-PCR and Western blot analyses.Central signal pathways were examined using Western blot analysis.
RESULTS. DNA microarray analysis showed alterations in the transcriptome of bladder epithelial cells infected with C. albicans over both the short and long terms. Key inflammatory and apoptosis networks were changed, which was consistent with several cellular events. Cellular levels of reactive oxygen species and nitrogen oxide increased after infection. Productions of cyclooxygenase-2 and prostaglandine E2 also increased after C. albicans infection, and their productions were suppressed by blockage of reactive oxygen species-epidermal growth factor receptor-Erk pathway.
CONCLUSIONS. This study provides evidence that C. albicans infection triggers inflammation and cellular damage by dysregulating key regulatory genes, signaling pathways, and bioactive species in normal bladder cells.
Marrow-Derived Mesenchymal Stem Cells Transfected with Survinvin Gene Protect Kidney from ischemia-reperfusion Injury in Rats
Authors: Xiaofeng Yang , Wang Qianqian
Published: September 2021

Objective: To investigate the survival ability of bone marrow Mesenchymal stem cells (MSCs) transfected with survinvin gene in the microenvironment of renal ischemia,and to study the ability and mechanism of repairing renal ischemia-reperfusion injury in rats.Method: Mesenchymal stem cells (MSCs) from bone marrow of male Sprague–Dawley rat were infected with the self‐inactive lentiviral vector and transfected with the Survinvin gene recombinant vector and then EGFP-tagged. After amplification and culture, they were detected by green fluorescence and then retained.48 specific pathogen-free C57BL/6J mice were randomly divided into 4 groups of 12 each. Rats in the control group were only surgically exposed. The other 3 groups were surgically exposed and the bilateral renal arteries were clamped for 45 minutes to restore blood supply, and models of renal ischemia-reperfusion were established. There were control group,ischemia reperfusion group(Marked as IR group), empty virus transfection transplantation group(Marked as MSCs group) or survinvin gene transfection transplantation group(Marked as SVV/MSCs group), and sequentially injected with normal saline,normal saline,1×106 MSCs infected with the self‐inactive lentiviral vector or 1×106 survivin gene-expressing MSCs. At different time points of 1d, 3d, 7d, 14d, collect serum to test blood urea nitrogen detection, to cut the rat kidney section for quantitative analysis, HE staining to observe renal issues changes and the degree of renal tubular damage and IL-10 by using ELISA detection. Result: The MSCs with resuscitation and expansion culture had strong proliferation and good fluorescence. The creatinine urea nitrogen level in the MSCs group and SVV/MSCs group was significantly lower than that in the IR group and control group (p<0.01 or p<0.05). The pathological damage score of HE staining in the kidney was lighter in the stem cell transplantation group, and the SVV/MSCs group was significantly lower than the other two groups (p<0.01 or p<0.05). On the 3rd and 14th day, the number of transplanted cells in the kidney tissue was much higher in the SVV/MSCs group than in the MSCs group. The MSCs expressing EGFP were mainly distributed around the glomerulus, the small vessel inner wall, and the interstitial between the renal tubule and the renal tubule. However, MSCs expressing EGFP were hardly seen on the inner wall of the renal tubule. The levels of protective factors IL-10 increased after renal ischemic injury. SVV/MSCs group was also significantly more than IR group or MSCs group (P<0.01 or p<0.05). And there was no statistical difference from the normal control group on the 14th day.Conclusion: Transfection of Survinifin gene can increase the survival ability of MSCs in ischemic kidney. After transplantation, MSCs are not directly differentiated into injured tubular endothelial cells, which further promote the repair of kidney damage through its strong paracrine effect.
Piezo1 is the cardiac mechanosensor that initiates the hypertrophic response to pressure overload
Authors: Yu Ze-Yan , Gong Hutao , Kesteven Scott , Guo Yang , Wu Jianxin , Li Jinyuan , Iismaa Siiri , Kaidonis Xenia , Graham Robert , Cox Charles , Feneley Michael , Martinac Boris
Published: September 2021

Pressure overload-induced cardiac hypertrophy is a maladaptive response with poor outcomes and limited treatment options. The transient receptor potential melastatin 4 (TRPM4) ion channel is key to activation of a Ca2+-calmodulin kinase II (CaMKII)-dependent hypertrophic signalling pathway after pressure overload, but TRPM4 is neither stretch-activated nor Ca2+-permeable. Here we show that Piezo1, which is both stretch-activated and Ca2+-permeable, is the mechanosensor that transduces increased myocardial forces into the chemical signal that initiates hypertrophic signalling via TRPM4. Cardiomyocyte-specific deletion of Piezo1 in adult mice prevented activation of CaMKII and inhibited the hypertrophic response: residual hypertrophy was associated with calcineurin activation in the absence of its usual inhibition by activated CaMKII. Piezo1 deletion prevented upregulation of the sodium-calcium exchanger and downregulation of the T-type calcium channel after pressure overload. These findings establish Piezo1 as the cardiomyocyte mechanosensor that instigates the maladaptive hypertrophic response to pressure overload, opening an avenue to novel therapies.
Selected ‘Starter Kit’ energy system modelling data for Venezuela (#CCG)
Authors: Cannone Carla , Allington Lucy , Pappis Ioannis , Barron Karla Cervantes , Usher Will , Pye Steve , Howells Mark , Walker Miriam Zachau , Ahsan Aniq , Charbonnier Flora , Halloran Claire , Hirmer Stephanie , Taliotis Constantinos , Sundin Caroline , Sridha Vignesh , Ramos Eunice , Brinkerink Maarten , Deane Paul , Gritsevskyi Andrii , Moura Gustavo , Rouget Arnaud , Rogner Holger , Quirós-Tortós Jairo , Angulo-Paniagua Jam
Published: September 2021

Energy system modelling can be used to assess the implications of different scenarios and support improved policymaking. However, access to data is often a barrier to energy system modelling, causing delays. Therefore, this article provides data that can be used to create a simple zero order energy system model for Venezuela, which can act as a starting point for further model development and scenario analysis. The data are collected entirely from publicly available and accessible sources, including the websites and databases of international organizations, journal articles, and existing modelling studies. This means that the dataset can be easily updated based on the latest available information or more detailed and accurate local data. These data were also used to calibrate a simple energy system model using the Open Source Energy Modelling System (OSeMOSYS) and three stylized scenarios (Fossil Future, Least Cost and Net Zero by 2050) for 2020–2050. The assumptions used and results of these scenarios are presented in the appendix as an illustrative example of what can be done with these data. This simple model can be adapted and further developed by in-country analysts and academics, providing a platform for future work.
Chlorpyrifos and Dichlorvos in Combined Exposure Reveals Antagonistic Interaction to Freshwater Fish Mrigal, Cirrhinus mrigala
Authors: Kunwar Prabesh Singh , Sapkota Bhawani , Badu Samikshya , Parajuli Kusum , Sinha Amit , De Boeck Gudrun , Sapkota Kumar
Published: September 2021

Toxicity imposed by organophosphate pesticides to the freshwater cultivable fish species mrigal (Cirrhinus mrigala) was assessed under laboratory conditions. Healthy juveniles were exposed to chlorpyrifos, dichlorvos, and their equitoxic mixture in geometric series. Median lethal concentrations of chlorpyrifos were found to be 0.906 (0.689–1.179), 0.527 (0.433–0.633), 0.435 (0.366–0.517) and 0.380 (0.319–0.450) mg/L and dichlorvos were found to be 38.432 (33.625–47.866), 22.477 (19.047–26.646), 12.442 (9.619–14.196) and 11.367 (9.496–13.536) mg/L after 24 h, 48 h, 72 h and 96 h of exposure respectively. Surprisingly, the joint toxicity of these organophosphates in the binary mixture was less than additive during most of the exposure periods. Behavioral changes exhibited by pesticide exposed fish were elevated opercular beatings, loss of equilibrium, loss of schooling behavior, aggregating at corners of the test chamber, slight color changes, surplus mucus secretion, and sudden and rapid body movements before death. Such behavioral studies can be applied as a non-invasive bio-monitoring tool for water quality assessment for fish growth and development. Despite the same mode of action of both pesticides, the antagonistic action in the binary mixture is an interesting outcome of this research that requires further investigation for a lucid understanding of the joint toxicity mechanism of such pesticides.
Efficacy of combined use of Xuebijing and ulinastatin for paraquat poisoning: A systematic review and meta-analysis of randomized controlled trials
Authors: xu yuqiong , Ding Xiaoyan , Li Rui , Long Xiang , Huang Wendi , Ding Jiezhu , Xu Yuqiong
Published: September 2021

Background: There are no clinically effective antidotes for paraquat poisoning, which progresses rapidly with a poor prognosis and high mortality. The addition of Xuebijing (XBJ) and ulinastatin (UTI) combination to conventional therapy has been reported as a novel treatment approach.Objective: To compare the effectiveness of Xuebijing combined with ulinastatin and conventional treatment group in the treatment of paraquat poisoning.Methods: The CNKI, VIP, WanFang, PubMed, EMBASE, and MEDLINE databases were searched for publications on randomized controlled clinical trials that compared the XBJ+UTI combination as an additional treatment to conventional therapy versus conventional therapy alone.Results: Quality assessment of the selected publications was performed and data were extracted for analysis. The final analysis included five publications, reporting 588 patients with paraquat poisoning. The control and XBJ+UTI groups comprised 294 patients each; 138 and 100 patients in the control and conventional therapy plus XBJ+UTI groups died, respectively. The combined analysis showed that the mortality relative risk (RR) was 0.72 and I2 was 46.2%. Three publications reported of pulmonary fibrosis (PF) and multiple organ dysfunction syndrome (MODS), in which 134 patients were included in each of the control and XBJ+UTI groups. MODS occurred in 117 and 88 patients in the control and conventional therapy plus XBJ+UTI groups, respectively. The combined analysis using the random-effects model showed that the RR was 0.75 and I2 was 0.0%. PF occurred in 99 and 68 patients in the control and conventional therapy plus XBJ+UTI groups, respectively. The combined analysis using the random-effects model showed that the RR was 0.69 and I2 was 0.0%.Conclusions: We preliminarily demonstrated that compared with those of conventional therapy alone, the addition of XBJ+UTI combination for the treatment of paraquat poisoning could reduce the mortality rate and incidence of MODS and PF.
A kutyatartás mint életmódot befolyásoló tényező vizsgálata
Authors: Vékony Blanka , Mák Erzsébet , Fónagy Orsolya
Published: September 2021

A XXI. században tapasztalható túlsúly és elhízás jelensége komoly népegészségügyi probléma. Megelőzése és kezelése is lehetséges, olyan életmódbeli faktorok változtatásával, mint fzikai aktvitás növelése és kiegyensúlyozot táplálkozás követése. Kutatások abba az irányba mutatnak, hogy a testmozgás kérdésében a kutyatartás egy serkentő tényező. Felmérésünk az előbbi kérdés, a táplálkozással és táplálással kapcsolatos ismeretek szintjének vizsgálatára vonatkozik. Kérdőíves módszert alkalmaztunk: a magyar, nagykorú kutyatartók (n=95) körében. Összességében a gazdák 79%-a legalább het egyszer végez testmozgást. A négylábú kedvenc, az egy hétre eső fzikai aktvitások 70%-ában játszik közre. Megállapítható, hogy a sport- és munka-kutyatartók táplálással kapcsolatos ismeretei szignifkánsan jobbak, szemben a hobbi-kutyatartókkal. A táplálkozás kérdésében nem sikerült hasonló kapcsolatot bizonyítani. A kutyatartás és testmozgás közöt összefüggés állapítható meg. Ennek pozitv hatása a BMI értékekben is megmutatkozik. A táplálás és táplálkozás kérdésében további kutatások szükségesek. Azonban, a kutyatartás nagy jelentőséggel bír az életmódbeli faktorok kedvező irányú befolyásolásának szempontjából.
An Assessment of the Seventh-Day Adventist Church Teacher Recruitment Process on the Achievement of the Church’s goals. A Study of Adventist Schools in Bulawayo
Authors: Dube Themba
Published: September 2021

This paper aimed at unearthing practices driven by the Adventist-Teacher recruitment process in order to determine achievement of the organization’s goals. The study was conducted in Adventist Schools in Bulawayo. The schools have a population of about 218 teachers. The study followed a mixed method of research, where quantitative-descriptive statistics were used to discuss findings. A sample of 106 teachers was chosen using the stratified sampling to respond to a self-administered questionnaire. Simple random was used to choose teachers from within each stratum among the three Secondary and Five Primary Schools. In the schools selected, the teachers’ list was used, and either odd numbers or even numbers were used as respondents. Through purposive sampling, three Secondary and five Primary School heads were chosen to respond to interview questions indicated in this study. It is important to note that teachers and school heads did not agree on issues regarding induction and mentoring. Primary data was collected and analyzed using SPSS. Tables and graphs were used in the presentation and discussion of findings. The study established the link between the Recruitment process- which aims at the integration of faith and learning- and Redemptive practices to be positive at Pearson’s r 0,696. The interaction of the Recruitment process and the cutting-edge practices recorded a Pearson’s r of 0,589. The recruitment process appeared to be operating below the ideal standard. The study revealed a poor teacher induction and mentoring process in the schools studied.
El camino a las redes neuronales artificiales
Authors: Restrepo Leal Diego Andrés , Viloria Porto Julie Pauline , Robles Algarín Carlos Arturo
Published: September 2021

En los últimos años se ha visto un acelerado crecimiento en el uso de las redes neuronales artificiales y la inteligencia computacional en el mundo. Esto obedece a diferentes factores como las mejoras a nivel de fabricación de los procesadores y la adopción de la filosofía del hardware y software libre, con lo cual se consolidaron comunidades cooperativas en torno a proyectos cada vez más ambiciosos e innovadores. Como resultado, han surgido diversas iniciativas que facilitan la implementación de redes neuronales artificiales, aunque a su vez se han convertido en una caja negra para los usuarios puesto que están conformadas por numerosos módulos que se traducen en muchas líneas de código por asimilar. Entendiendo la importancia de forjar bases sólidas de conocimiento, los autores realizan a lo largo de esta obra un recorrido de diversos conceptos y herramientas matemáticas para sumergir poco a poco al lector en la creación de sus propias utilidades para implementar redes neuronales, con un lenguaje amigable en el que se abordan numerosos ejemplos prácticos, los cuales están disponibles en una máquina virtual y en un repositorio público.
GRAVIDEZ NA ADOLESCÊNCIA: UMA ANÁLISE DO PERFIL DAS ADOLESCENTES ASSISTIDAS EM HOSPITAL ESCOLA NA CIDADE DE MACEIÓ-AL
Authors: Vasconcelos de Carvalho Renata , Carvalho Miranda Isis , Da Rocha Moraes Antônio Carlos , Gomes Alvim Ronaldo
Published: September 2021

Introdução: A adolescência é um período curto, porém é nela que ocorrem mudanças biológicas, físicas e psicológicas determinantes na busca da autoafirmação e que podem acarretar complicações no ciclo de vida. A gravidez vista nesta etapa é uma realidade no Brasil e, apesar de susceptível em qualquer classe social, os fatores econômico e social são determinantes nos índices estatísticos. Objetivo: Analisar o perfil das adolescentes que utilizam os serviços do ambulatório e enfermaria de obstetrícia do Hospital Veredas, identificar os fatores que levaram a uma maternidade precoce, as relações sociais, familiares e escolares das adolescentes, além das suas expectativas futuras na construção afetiva da relação mãe-filho e dos possíveis riscos gerados à saúde de ambos. Metodologia: Pesquisa analítica, individual, transversal e observacional, com dados coletados através de análise de prontuários para seleção da amostra e entrevistas com as gestantes do ambulatório e enfermaria de obstetrícia do Hospital Veredas, Maceió-AL, entre Janeiro de 2020 e Novembro de 2020.  Resultados e Discussão: Das adolescentes analisadas, 52,3% eram menores de idade, dado significativo que demonstra a natureza social destas gestações. Quando questionadas sobre sua percepção quanto a gestação, 57,5% declararam que se tratava de uma gravidez indesejada, informação que associada a narrativa dessas mulheres, demonstra que a gravidez associada a ausência de uma rede de apoio, agrava sua rejeição, refletindo em um isolamento e dificuldade de aceitação. Pode-se constatar através de análise inferencial que uma tendência das adolescentes etilistas evoluírem com gestações pré-termo (tempo de gestação < 37 semanas) e recém-nascidos prematuros. Conclusões: Através da análise dos dados e da bibliografia analisada, nota-se que a gravidez na adolescência é uma realidade no município de Maceió, e permanece um importante problema de saúde pública gerando impactos negativos nos aspectos sociais, econômicos e nos indicadores de saúde dessas jovens.

 

Palavras-Chave: Família; Gravidez na adolescência; Sexualidade.
REABILITAÇÃO ESTÉTICA COM RESINA INDIRETA EM DENTES ANTERIORES: UM RELATO CLÍNICO
Authors: Da Nóbrega Dias Joselúcia , Larissa Araújo Pereira e Souza Endyara , José Souza dos Santos Alex , Vieira de Assunção Isauremi
Published: September 2021

Introduction: The aesthetic rehabilitation of endodontically treated teeth depends on the degree of destruction of the crown, the tooth in question, the bone support, the type of prosthesis and the types of forces to which these teeth will be submitted. The indirect composite resins have improved physical and mechanical properties thanks to the incorporation of inorganic components and multifunctional monomers with a greater number of bonding points, making them capable of solving the problems in which ceramic restorations would be indicated. Objective: To present a clinical case report of an aesthetic rehabilitation of anterior teeth with indirect composite resin as a viable and less expensive treatment alternative. Case Report: Upon clinical examination, extensive and deficient restorations were observed in the upper anterior teeth. The proposed treatment plan aesthetic rehabilitation of dental elements with ceromeres. Conclusions: This treatment represented a viable and less expensive option that can be used both in situations of great dental destruction and in small dental absences, in substitution for ceramic restorations.
A VISITA DOMICILIAR DO ENFERMEIRO DA ESTRATÉGIA SAÚDE DA FAMÍLIA NA ATENÇÃO AO PUERPÉRIO
Authors: De Lima Claudia Silva , César Vieira de Araújo Túlio
Published: September 2021

Introdução: Qualquer atendimento realizado pela Estratégia Saúde da Família no domicílio é definido como visita domiciliar, o puerpério é um dos momentos que requer uma assistência domiciliar qualificada e oportuna haja vista as peculiaridades que envolvem o binômio materno infantil. Objetivo: Descrever as principais ações abordadas na literatura sobre a visita domiciliar do enfermeiro da Estratégia Saúde da Família no período puerperal. Metodologia: Trata-se de uma revisão integrativa de literatura, a busca pelo material ocorreu nas bases de dados, Literatura Latino Americana e do Caribe em Ciências da Saúde, Scientific Electronic Library Online, Medical Literature Analysis and Retrieval System Online e a Base de Dados em Enfermagem, entre os meses de agosto e setembro de 2020, com os descritores Visita Domiciliar, Período Pós-Parto, Estratégia Saúde da Família e Enfermeiro. Foram identificados 53 artigos potencialmente relevantes com base na leitura do título e resumo, após uma leitura detalhada dos estudos, 10 foram selecionados Resultados: A visita domiciliar do enfermeiro no período puerperal está muito aquém do que é preconizado nas recomendações do Ministério da Saúde, a literatura constata a ausência das visitas domiciliares, ou quando realizadas não ocorriam na primeira semana pós alta hospitalar, nem contemplavam as diretrizes recomendadas para o atendimento domiciliar. Conclusões: As principais ações relacionadas a visita domiciliar do enfermeiro no período puerperal apontadas na literatura giram em torno dos cuidados prestados; das percepções das puérperas e da relevância da visita domiciliar com destaque para falhas na integralidade da assistência domiciliar ao binômio mãe e filho.
¿Existe una Bibliotecología desde Nuestra América? Los problemas de “la identidad desde la exclusión” y el “saber desde la inclusión”
Authors: Morán-Reyes Ariel Antonio
Published: September 2021

En este artículo se exploran algunas condiciones de posibilidad sobre un pensamiento bibliotecológico en América Latina. Para tales fines, se analizó la propuesta de una “Bibliotecología desde Abya Yala”, sobre todo por lo desafiante de sus pretensiones y porque exhibe algunos síntomas generalizados en esta clase de ideaciones (como inconsistencias conceptuales y metodológicas derivadas de prejuicios identitarios regionalistas). Se identificaron dos problemas básicos: “la identidad desde la exclusión” y el supuesto estado epistémico de “saber desde la inclusión”. La “identidad desde la exclusión” se refiere al problema de intentar definir la identidad latinoamericana desde lo “no anglosajón”, y por el criterio de descarte de ideas ajenas como parte de las acciones para lograr, en modo artificial, el carácter “latinoamericano” auténtico (sin que exista de por medio una justificación epistémica). Por otro lado, el problema del supuesto “saber desde la inclusión” (que parte, más bien, desde la exclusión y la marginación hermenéutica) retoma los mismos prejuicios identitarios, y se posa, indirectamente, en el arcaico pensamiento funcionalista bibliotecológico. A pesar de esto, su rasgo más polémico resulta ser que se basa en estados epistémicos deterministas que no son sostenibles ni ética ni epistemológicamente (ni siquiera en la esfera práctica más inmediata).
Diálogos entre a informatika e a ciência da informação
Authors: Mello Mariana Rodrigues Gomes de , Martínez-Ávila Daniel
Published: September 2021

De um lado a Ciência da Informação foi se consolidando no eixo occidental, a partir década de 1960, expandindo sua influência inteletual em países da America Latina, substuindo uma tradição francesa-europeia de Documentação, e como meio de controle ideológico e da informação; do outro surge uma proposta reativa do eixo comunista, a Informatika. Posto isto, pretende-se neste ensaio criar lugar pra reflexão acerca de alguns pontos em que a Informatika influenciou a Ciência da Informação ocidental, mais precisamente a estadunidense, sobretudo nas primeiras décadas da Guerra Fria, como também o inverso, ou seja, as influências da CI dos Estados Unidos à Informatika. Para tanto, iniciaremos nossas reflexões a partir de duas escolas: a da Teoria Matemática da Comunicação e da Epistemologia Social. Posto isto, o problema de pesquisa incide na seguinte questão: Apesar dos aspectos mercadológicos e ideológicos tão distintos dos blocos capitalista e socialista, é possível verificarmos algumas convergências teóricas na construção da Ciência da Informação e da Informatika? Metodologicamente, trata-se de uma pesquisa bibliográfica, com aspectos exploratórios. Conclui-se que apesar da União Soviética apresentar distintamente a informação social e tê-la como algo importante ante a ideologia do partido socialista, foi visto que a informação científica e técnica também foi muito aprimorada e valorizada pelos soviéticos. Assim como os Estados Unidos não priorizaram sempre o aspecto técnico da informação. As duas vertentes – social e técnica - caminharam juntas, ora predominando mais uma, ora outra, nos dois blocos, dependendo do momento e dos objetivos.
Drug Treatment of Epilepsy: From Serendipitous Discovery to Evolutionary
Mechanisms
Authors: Lou Shengying , Cui Sunliang
Published: September 2021

Epilepsy is a chronic brain disorder caused by the abnormal firing of neurons.
Up to now, the use of antiepileptic drugs is the main method of epilepsy treatment. The
development of antiepileptic drugs lasted for centuries. In general, most agents entering
clinical practice act on the balance mechanisms of brain “excitability-inhibition”. More
specifically, they target voltage-gated ion channels, GABAergic transmission and glutamatergic
transmission. In recent years, some novel drugs representing new mechanisms
of action have been discovered. Although there are about 30 available drugs in the market,
it is still in urgent need of discovering more effective and safer drugs. The development
of new antiepileptic drugs is into a new era: from serendipitous discovery to evolutionary
mechanism-based design. This article presents an overview of drug treatment of
epilepsy, including a series of traditional and novel drugs.
Strategy of Virtual Screening based Discovery of HSP90 C-terminal Inhibitors and Network Pharmacological Analysis
Authors: Liu Yajun , Li Lihong , Yang Man , Li Chenyao , Xue Hongyu , Shi Meiyun
Published: September 2021

Background:

HSP90 has been considered an important anticancer target for several decades, but traditional HSP90 N-terminal inhibitors often suffered from organ toxicity and/or drug resistance.

Methods:

The development of HSP90 C-terminal inhibitors represents a reliable alternative strategy. In view of rare examples of structure-based identification of HSP90 C-terminal inhibitors, we report a virtual screening based strategy for the discovery of HSP90 C-terminal inhibitors as anticancer agents from natural products.

Results & Discussion:

13 chemical ingredients from licorice were identified as possible HSP90 inhibitors and 3 of them have been reported as anticancer agents. The binding modes towards HSP90 C-terminus were predicted by molecular docking and refined by molecular dynamics simulation.

Conclusion:

Further network pharmacological analysis predicted overall possible targets involved in the pathways in cancer and revealed that 8 molecules possibly interact with HSP90. A structure based virtual screening strategy was established for the discovery of HSP90 Cterminal inhibitors.
The Role of Platelet in Severe and Fatal Forms of COVID-19
Authors: Esparza-Ibarra Edgar L. , Ayala-Luján Jorge L. , Mendoza-Almanza Brenda , González-Curiel Irma , Godina-González Susana , Hernández-Barrales Marisa , Mendoza-Almanza Gretel
Published: September 2021

:

On December 31, 2019, the World Health Organization received a report of
several pneumonia cases in Wuhan, China. The causative agent was later confirmed as
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Since then, the
SARS-CoV-2 virus has spread throughout the world, giving rise in 2020 to the 2019
coronavirus (COVID-19) pandemic, which, according to the world map of the World
Health Organization, has, until May 18, 2021, infected 163,312,429 people and caused
3,386,825 deaths throughout the world. Most critical patients progress rapidly to acute
respiratory distress syndrome (ARDS) and, in underlying form, septic shock, irreversible
metabolic acidosis, blood coagulation dysfunction, or hemostatic and thrombotic
anomalies have been reported as the leading causes of death due to COVID-19. The
main findings in severe and fatal COVID-19 patients make it clear that platelets play a
crucial role in developing severe disease cases. Platelets are the enucleated cells
responsible for hemostasis and thrombi formation; thus, platelet hyperreactivity induced
by pro-inflammatory microenvironments contributes to the "cytokine storm" that
characterizes the more aggressive course of COVID- 19.
Nanotherapeutics for Alzheimer's Disease with Preclinical Evaluation and
Clinical Trials: Challenges, Promises and Limitations
Authors: Bukhari Syed Nasir Abbas
Published: September 2021

Alzheimer’s Disease (AD), a progressive and irreversible neurodegenerative disorder, is
the most common form of dementia worldwide. Currently, there is no disease-modifying AD drug,
and the development of effective treatments is made even harder by the highly selective nature of
the Blood-Brain Barrier (BBB) that allows the passage only of molecules with specific chemical--
physical properties. In this context, nanomedicine and its Nanoparticles (NPs) offer potential solutions
to the challenge of AD therapy, in particular, the requirements for i) BBB crossing, ii) multitarget
therapy iii) enhancement of pharmacokinetics; and iv) more precise delivery. In addition, the
possibility to optimize NP biophysical and biological (i.e. target-specific ligands) properties allows
for highly tailored delivery platforms. Preclinical studies have demonstrated that nanotherapeutics
provide superior pharmacokinetics and brain uptake than free drugs and, on the other hand, these
are also able to mitigate the side-effects of the symptomatic treatments approved by the FDA.
Among the plethora of potential AD nanodrugs, multitarget nanotherapeutics are considered the
most promising strategy due to their ability to hit simultaneously multiple pathogenic factors,
while nano-nutraceuticals are emerging as interesting tools in the treatment/prevention of AD. This
review provides a comprehensive overview of nanomedicine in AD therapy, focusing on key optimization
of NPs properties, most promising nanotherapeutics in preclinical studies and difficulties
that are limiting the efficient translation from bench to bedside.
Characteristics of HIV-related Stigma Among Health Facility Staff in Shenzhen, China
Authors: Wang Wei , Wang Liling , Wang Jun , Zhu Naling , Luo Yali , Tang Weiping , Qiao Yaping
Published: September 2021

Background:

HIV-related stigma poses a crucial barrier to HIV therapy and prevention
worldwide. Stigma taking place in healthcare settings has also been a global challenge for years.

Objective:

To measure HIV-related stigma among health care facilities in Baoan, Shenzhen and to
take decision regarding stigma-reduction intervention.

Methods:

We conducted a cross-sectional survey in Shenzhen Baoan Women's and Children's Hospital,
China in February 2019. We collected data on HIV-related stigma from 207 hospital staff randomly
selected for an anonymous survey online by a standardized questionnaire. We analyzed the
influence factors on HIV-related stigma taking place in health facilities using the statistical
method.

Results:

The percentages of worry expressed when touching clothing, dressing wound, and drawing
blood for PLWH were 60.9%, 84.5%, and 82.6%, respectively. 76.3% of respondents reported
at least one form of extra precautions taken during service provision to PLWH. Younger hospital
employees with less working experience were more likely concerned about occupation risk of HIV
infection (P<0.05). Single workers with lower education and no experience of training on HIV-related
stigma were prone to discriminatory opinions against PLWH (P<0.05).

Conclusion:

HIV-related stigma in health facilities remains a significant challenge, and evidence-
based interventions at both systematic and individual levels are needed for improvements.
Evaluation of a Novel In-house HIV-1 Genotype Drug Resistance Assay
using Clinical Samples in China
Authors: Gao Peijie , Yu Fengting , Yang Xiaozhen , Li Dan , Shi Yalun , Wang Yan , Zhang Fujie
Published: September 2021

Background:

HIV drug resistance poses a major challenge for anti-retroviral treatment
(ART) and the prevention and control of HIV epidemic.

Objective:

The study aims to establish a novel in-house assay with high efficiency, named AP inhouse
method, that would be suitable for HIV-1 drug resistance detection in China.

Methods:

An in-house HIV-1 genotyping method was used to sequence the partial pol gene from
60 clinical plasma samples; the results of our test were compared with a commercial ViroSeq
HIV-1 genotyping system.

Results :

Among sixty samples, 58(96.7%) were successfully amplified by AP in-house method,
five of them harbored viral load below 1,000 copies/ml. The genotype distribution was 43.1% CRF07_
BC (25/58), 39.7% CRF01_AE (23/58), 6.9% CRF55_01B (4/58), 5.2% subtype B (3/58) and
5.2% CRF08_BC (3/58). Compared with that of the ViroSeq system, the consistent rate of these nucleotides
and amino acids obtained by AP in-house method was up to 99.5 ± 0.4% and 99.5 ±
0.4%, respectively. A total of 290 HIV-1 drug resistance mutations were identified by two methods,
including 126 nucleoside reverse transcriptase inhibitors (NRTIs), 145 non-nucleoside reverse
transcriptase inhibitors (NNRTIs) and 19 protease inhibitors (PIs) resistance mutations. Out of
them, 94.1% (273/290) were completely concordant between the AP in-house method and the ViroSeq
system.

Conclusion:

Overall, the evaluation of AP in-house method provided comparable results to those
of the ViroSeq system on diversified HIV-1 subtypes in China.