bluuebunny/crossref_metadata_2025 huggingface dataset:
Research Progress and Mechanisms of Acupuncture Combined with Transcutaneous Vagal Nerve Stimulation in the Treatment of Cognitive Impairment after Stroke
Authors: Yang Shan , Gong Yongjie , Wu Kai , Lei Haoxing , Yu Yalan
Published: November 2024

Cognitive impairment is a common complication after stroke, which not only affects the rehabilitation process of patients but also increases the family and socio-economic burden. Finding an effective treatment for cognitive impairment after stroke is urgent. Acupuncture can effectively activate blood circulation, dredge collaterals, tonify qi and blood, and regulate yin and yang. It is an effective method for the treatment of cognitive impairment after stroke. Recent studies have shown that acupuncture combined with various modern rehabilitation techniques, such as transcutaneous vagus nerve stimulation, has better clinical effects than either acupuncture or rehabilitation therapy alone. This article reviews the clinical research and basic mechanisms in this field by searching Chinese and English literature from the past 20 years, aiming to provide research ideas for future studies on the combined treatment of post-stroke cognitive impairment and related mechanisms.
Research on Teaching Reform of Pathology Courses in Colleges and Universities under the Background of “Internet plus New Medical Science”
Authors: Hong Ziwei
Published: November 2024

With the advent of the era of “Internet plus New Medical Science,” the teaching mode for pathology courses in colleges and universities faces unprecedented opportunities for transformation. In this context, a pathology teaching model centered on theoretical explanations alone can no longer fully meet the demands of modern medical education. Therefore, it is essential to study and explore pathways for reforming pathology teaching in higher education within the framework of “Internet plus New Medical Science.” Research reveals a series of issues in current pathology teaching in universities, such as the uneven distribution of teaching resources, limited teaching methods, a disconnect between theory and practice, and incomplete evaluation systems. To address these challenges, universities should adopt a series of measures: optimizing resource allocation, innovating teaching methods and approaches, strengthening the integration of theory and practice, enhancing evaluation systems, and building robust teaching teams. These reforms aim to stimulate students’ interest in learning and improve the effectiveness of pathology instruction.
Care-educational technologies used in primary health care in women's health care: scope review
Authors: Januário Rafaela Amaro , Cavalcanti Ana Clara de Sousa , Almeida Layane Ruth Jeremias , Assis Alessandra Emilly Pinto de , Vieira Cicera Renata Diniz , Nascimento Maria Berenice Gomes
Published: November 2024

Objective: To map and identify the care and educational technologies used in Primary Health Care (PHC) in women's health care. Methods: A scoping review was carried out based on the Joanna Briggs Institute manual and guided by the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) Extension for Scoping Reviews. A three-stage comprehensive systematic search was conducted from February to April 2023 in multidisciplinary health sciences databases and gray literature. Data extraction and eligibility were carried out by two authors independently and interpreted using thematic analysis. Results: included 15 studies and identified two thematic groups: Types of care-educational technologies and Themes addressed in the technologies. Conclusion: highlighted the types of care-educational technologies that are used in PHC as fundamental tools for teaching and assisting women's health, making them protagonists in the construction of the health-disease process.
„Tak naprawdę to zima wykończyła rząd Gierka” - narracje klimatyczne dwóch pokoleń kobiet
Authors: Kopciewicz Lucyna
Published: November 2024

Celem artykułu jest poddanie dyskusji wyników badań empirycznych poświęconych narracjom klimatycznym. W badaniach uczestniczyło 35 kobiet repezentujących najstarsze pokolenie (75-87 lat) oraz 35 dziewcząt reprezentujących pokolenie piętnastolatek. Zastosowana jakościowa procedura badawcza – wywiad, umożliwiła zebranie narracji. Problemy badawcze zawarte były w formule następujących pytań:
Jaki retrospektywny obraz zimy jako pory roku wyłania się z narracji uczestniczek badań empirycznych?
Jaki obraz ewentualnych zmian klimatycznych wyłania się z narracji kobiet uczestniczących w badaniu?   
Ramą teoretyczną projektu jest paradygmat emocjonalizacji zmian klimatu. Wyniki badań obejmują zidentyfikowane znaczenia zimy jako pory roku, analizę wyłaniających się istotnych kategorii opisu oraz analizę emocji klimatycznych uczestniczek badania.
Odwrócenie symboliczne w narracjach praktyków Montessori – dopiski do „Dyskursywnej konstrukcji podmiotu”
Authors: Jendza Jarosław
Published: November 2024

W prezentowanym artykule, stanowiącym część większego projektu badawczego, autor analizuje fragmenty narracji nauczycielek Montessori (pracujących w polskich placówkach tego typu) odnośnie do kwestii dzieciństwa oraz traktujące o symbolicznym odwróceniu obecnych w ich wypowiedziach. Wykorzystując procedurę fenomenografii oraz narzędzi krytycznej analizy dyskursu autor stawia pytania badawcze, o to, co ulega znaczeniowej inwersji w pedagogii Montessori, oraz jakie aspekty jej funkcjonowania pozostają niezmienne. Tym samym celem badania stanowi identyfikacja i opis odwróconych i niezmienionych aspektów edukacji na pozimie przedszkola. Tak postawiony cel badań oraz procedura doprowadzają do wytworzenia interesującego przedmiotu poznania. W narracjach pedagożek Montessori symbolicznemu odwróceniu podlegają niektóre rozwiązania metodyczne, organizacyjne i komunikacyjne, ale względnie stała jest dystrybucja władzy w edukacji oraz jej zasadniczy cel. Wyniki badania pokazują także, że kategoria symbolicznego odwrócenia ma swoje ograniczenia, zwłaszcza w sytuacji, gdy mamy do czynienia z przesunięciem akcentu, raczej niż inwersją porządku.
Promoters of Carbon Dioxide Hydrate Formation Derived from Citric and Amino Acids
Authors: Gainullin S. E. , Kazakova P. Yu. , Pavelyev R. S. , Tavochkin V. M. , Varfolomeev M. A.
Published: November 2024

Novel amides of some amino and citric acids were synthesized and studied for their ability to improve the kinetics of carbon dioxide hydrate formation. It was demonstrated that these compounds can be effective promoters of hydrate formation, even at low concentrations. The addition of citric acid and norleucine amide (CTR+Nle) increased the water-to-hydrate conversion and significantly reduced the induction time. At a concentration of 0.05 wt. %, the conversion rate reached 57 ± 3 %, which is 21 and 3.1 % higher than in water without additives and sodium dodecyl sulfate (SDS) solution, respectively. In the presence of CTR+Nle, the induction time decreased to 35 min, which is an 8.1-fold reduction compared to water without additives, as well as 5.8-fold less than with tryptophan solution known as a promoter of carbon dioxide hydrate formation.
A New Class of Generalized Starlike Bi-Univalent Functions Subordinated to Legendre Polynomials
Authors: Al-Rawashdeh Waleed
Published: November 2024

A new class of generalized starlike bi-univalent functions is introduced in this paper, which is defined using Legendre Polynomials within the open unit disk D. This paper sheds a light on the properties and behaviors of these starlike bi-univalent functions, providing estimations for the modulus of the initial Taylor series coefficients of the functions falling under this particular class and one of its various subclasses. Additionally, this paper also investigates the classical Fekete-Szegö functional problem for functions f that are part of the aforementioned class. Moreover, we obtain the classical Fekete-Szegö inequalities of functions belonging to this class and to one of its various subclasses.
Decision-Making of a New Type of Stochastic Space and Its Associated Operator Ideal
Authors: Alshabhi Salah H. , Baruah Achyutananda , Mohammed Mustafa M. , Magzoub Mona , Taha Khdija O. , Bashir Runda A. A. , Hamed Sakeena E. M. , Tripathy Binod Chandra , Bakery Awad A.
Published: November 2024

We develop and examine the pre-modular space of null variable exponent-weighted backward generalized difference gai sequences of fuzzy functions in this paper. These sequences of fuzzy functions are important contributions to the concept of modular spaces because they have exponent weighting. Using extended s−fuzzy functions as well as this sequence space of fuzzy functions, it has been possible to accomplish an idealization of the mappings. We have presented some topological and geometric properties of this new space, as well as the ideal mappings that correspond to them.
The Twentieth Free Software Developers Conference
Authors: Cherny V.
Published: November 2024

The book contains conference proceedings approved by the Program Committee of the Twentieth Free Software Developers Conference.
Sedimentary rocks – 2022
Authors: Rostovtseva Yu.
Published: November 2024

The collection contains materials of the reports of the scientific readings «Sedimentary rocks - 2024», held in 2024, dedicated to the 80-th anniversary of the founding of the «Sedimentary rocks» section of the Moscow Society of Naturalists (MSN). A wide range of issues related to the study of sedimentary rocks (exoliths) of various genesis and ages, which have both fundamental scientific and applied significance, are considered. The collection of materials is of interest to geologists of various specialties who are engaged in the complex studies of the upper part of the lithosphere, as well as in the detailed lithological studies.
Avoidant Coping as Moderator of Risk Percetion and Anxiety in Pandemic Context
Authors: Mirón Juárez Carlos Alberto , Díaz Grijalva Giovana Rocío , Ochoa Avila Eneida , Mercado Ibar Santa Magdalena
Published: November 2024

Risk perception plays a crucial role in predicting both anxiety and protective behaviors amidst uncertainty. Drawing from Lazarus and Folkman's transactional theory, maladaptive coping strategies are suggested to moderate the relationship between risk perception and anxiety. This study aimed to examine the moderating effect of avoidant coping strategies on anxiety levels predicted by risk perception during the Covid-19 pandemic. A sample of 395 individuals with potential Covid-19 comorbidities, averaging 37.5 years, participated in non-experimental assessments using psychometric scales to measure anxiety, Covid-19 risk perception, avoidant coping, and protective behaviors. Comparative analyses by age and gender were conducted alongside structural modeling employing partial least squares to explore the moderating role of avoidant coping. Results revealed a significant positive effect of avoidant coping strategies on increasing anxiety predicted by risk perception. These findings underscore the importance of fostering healthier coping mechanisms in future public health initiatives to mitigate the adverse impacts of risk communication regarding disease threats.
Complete systems of invariants of a hyper-surface in a Euclidean space
Authors: Khadjiev D.
Published: November 2024

Let M(n+1) be the group of all isometric transformations of an n+1-dimensional
Euclidean space Rn+1, SM(n + 1) is the group of all Euclidean motions of Rn+1, U is the domain f(u1)2 + · · · + (un)2 < 1g in Rn and x(u) is an U-hyper-surface (that is a C1-mapping
x : U ! Rn+1) in Rn+1. Let x(u) be a regular hyper-surface, I(x) = Pn i;j=1 gij(x)duiduj
and II(x) = Pn i;j=1 Lij(x)duiduj are the first and second fundamental forms of x. Put
K = f(i; j) : 1 ≤ i ≤ j ≤ ng. We consider the following problem: find a proper (minimal,
it is desirable) subset T of K such that equalities gij(x)(u) = gij(y)(u); Lij(x)(u) = Lij(y)(u)
for all (i; j) 2 T and u 2 U implies an existence of F 2 SM(n + 1) such that y(u) = Fx(u)
for all u 2 U. For a solution of this problem, we have obtained a generating system of the
differential field of all G-invariant differential rational functions of a hyper-surface in Rn+1 for
groups G = M(n + 1); SM(n + 1). In our result, jTj = n(n+1)
2 + n, where jTj is the number of
elements of T.
A SAÚDE MENTAL NO AMBIENTE ESCOLAR: CONEXÕES ENTRE A ADOLESCÊNCIA, A ESCOLA E A FAMÍLIA
Authors: Lobato Geórgia Rosa , Reis Annita Fundão Carneiro dos , Pinheiro Bruna Maia , Menezes Diego Prata Pereira de , Barreto Isabelle , Araujo Ana Maria Pereira Brasilio de , Landi Laura Corrêa de Magalhães
Published: November 2024

O presente artigo analisa as atividades realizadas pelo projeto de Extensão Adolescer II, conduzido no Centro Educacional Serra dos Órgãos (CESO). Adotou-se uma abordagem interprofissional, englobando estudantes e docentes dos cursos de Medicina e Psicologia do UNIFESO. Os objetivos principais foram identificar questões relevantes que contribuem para o sofrimento psíquico entre os adolescentes e a equipe docente e construir ferramentas em colaboração com a comunidade escolar visando abordar as questões de saúde mental identificadas e seu enfrentamento. Utilizou-se a metodologia da cartografia para o acompanhamento dos processos e a produção de subjetividade, adotando a abordagem de pesquisa-intervenção. Um estudo de análise qualitativa foi conduzido para o tratamento e interpretação dos dados. Acredita-se que o suporte em saúde mental no ambiente escolar proporciona um espaço reflexivo sobre os processos de subjetivação, permitindo melhorias na qualidade de vida e redução de possíveis impactos nas relações e na aprendizagem decorrentes dos sofrimentos vivenciados. Concluiu- se que as atividades extensionistas do “Adolescer II” proporcionaram a construção de novos conhecimentos, habilidades e atitudes sobre saúde mental e adolescência e contribuiu com o exercício teórico-prático entre diferentes profissões da área da saúde com vista a qualificar a atenção à saúde da comunidade.
Ciências, sociedade e tecnologia: uma abordagem multidisciplinar - Volume 1
Authors: Coelho Glauber Tulio Fonseca , Araújo Filho Patrício Moreira de , Mendonça Pinheiro Eduardo
Published: November 2024

Bem-vindos ao livro "Ciências, Sociedade e Tecnologia". Esta obra explora a interconexão entre ciência e sociedade, abordando temas como:
Principais Tópicos:
- Intersecção entre ciência e sociedade
- Influência da tecnologia
- Educação científica
- Implicações éticas do progresso científico
Características:
- Abordagem interdisciplinar
- Conhecimentos de diversas áreas científicas.
A INFLUÊNCIA DAS REDES SOCIAIS NA FORMAÇÃO DA IDENTIDADE CONTEMPORÂNEA: UMA ANÁLISE FILOSÓFICA E SOCIOLÓGICA
Authors: Nascimento Suzy Carla Lima do
Published: November 2024

Este artigo examina o impacto das redes sociais na construção da identidade individual e coletiva na sociedade contemporânea. Através de uma análise sociológica aprofundada, investigamos como plataformas digitais como Facebook, Instagram e Twitter moldam a percepção de si e do outro, influenciando comportamentos, valores e relações interpessoais. O estudo aborda as transformações nas dinâmicas sociais, a fragmentação da identidade e a criação de personas online, destacando as implicações para a coesão social e o bem-estar psicológico dos indivíduos. Metodologicamente, baseia-se em uma revisão crítica da literatura existente e na análise de dados qualitativos e quantitativos sobre o uso de redes sociais. Os resultados apontam para uma reconfiguração significativa dos processos de socialização e formação identitária, evidenciando tanto oportunidades quanto desafios para a compreensão do self na era digital.

Palavras-chave: Redes Sociais. Identidade. Sociologia Digital. Contemporaneidade. Interação Social.
PROJETOS DE CIRCUITOS DIGITAIS COMBINACIONAIS COM FPGA UTILIZANDO LÓGICA DE PROCESSAMENTO PARALELO
Authors: Melo Ismael Silva e
Published: November 2024

Os FPGAs (Field Programmable Gate Arrays), são dispositivos de hardware reconfiguráveis que possibilitam a implementação de circuitos lógicos personalizados. O primeiro FPGA comercial foi lançado em 1985, revolucionando o desenvolvimento de hardware. Os FPGAs permitem que um hardware seja ajustado ou alterado sem a necessidade de fabricar um novo produto. A arquitetura de um FPGA é composta por blocos lógicos programáveis que executam funções específicas e por interconexões configuráveis, que roteiam os sinais entre os blocos, conferindo ao FPGA a capacidade de reconfiguração, o que permite que os FPGAs sejam ideais para aplicações que precisam de modificações ou aprimoramentos frequentes no design do hardware ao longo do tempo, sem substituir fisicamente o dispositivo. Com o avanço da tecnologia, os FPGAs têm evoluído em complexidade e capacidade, tornando-se uma solução poderosa para diversas aplicações em que a flexibilidade e a possibilidade de atualização são essenciais. Neste artigo, foram produzidos dois circuitos utilizando-se o FPGA e condensados em um único código, permitindo processamento simultâneo no FPGA. Simulações confirmaram que as saídas desses dois circuitos seguem combinações lógicas das entradas, conforme a tabela verdade e as formas de onda simuladas. Este método economiza tempo e recursos, além de ser flexível para múltiplas aplicações, bastando programar e endereçar entradas e saídas. O FPGA, com blocos independentes, facilita a integração e controle de diferentes circuitos.

Palavras-chave: FPGA, eletrônica, circuitos eletrônicos
ATUAÇÃO DO ENFERMEIRO NA CONSULTORIA DO ALEITAMENTO MATERNO
Authors: Belfort Alda Lourdes Batista , Silva Deilce Santos Da , Apoliano Geovanna , Santos Lilian Lopes Dos , Silva Maria Gabriele Diniz Da , Santos Soraya Andrade , Marins Josafá Barbosa
Published: November 2024

Introdução: Este artigo investiga as práticas dos enfermeiros na consultoria do aleitamento materno, contextualizando a teoria na prática do cuidado à saúde materno-infantil. Surge a questão sobre quais estratégias os profissionais de enfermagem utilizam para promover a amamentação e quais barreiras enfrentam em sua atuação. Objetivo: Analisar as contribuições dos enfermeiros para a promoção do aleitamento e identificar os obstáculos que dificultam uma atuação efetiva na consultoria de amamentação. Material e Métodos: O estudo é de natureza bibliográfica e descritiva, baseado na revisão de sete artigos previamente selecionados e publicados em plataformas digitais. Resultados: Os enfermeiros desempenham um papel essencial na promoção do aleitamento materno, utilizando técnicas de orientação e apoio emocional, além de abordagens educativas para superar barreiras. A literatura destaca a importância da formação contínua e do trabalho em equipe na efetividade das práticas de amamentação. Conclusão: A atuação dos enfermeiros na consultoria do aleitamento materno é crucial para o fortalecimento da amamentação, especialmente no que diz respeito à superação de desafios enfrentados pelas mães. A formação contínua desses profissionais e a implementação de estratégias inovadoras são fundamentais para aprimorar as ações de promoção da amamentação e, consequentemente, melhorar a saúde materno-infantil.

Palavras-chave: Aleitamento materno; Consultoria; Enfermagem; Promoção da saúde.
A PRÁTICA DE BIOSSEGURANÇA PARA PROFISSIONAL DE ENFERMAGEM
Authors: Sousa Amanda Da Silva Soares De , Azevedo Daniela Vieira , Sousa Maria Eduarda Da Costa , Santana Maria Eduarda Lopes De Oliveira , Sousa Sara Dos Reis Soares , Oliveira Sarah Kelvia Silva , Martins Thayná Kelly Lima , Cruz Vitória Teixeira Da , Rodrigues Xayanne Das Mercês Siqueira , Souza Mariane de Amarante
Published: November 2024

Introdução: A prática da Biossegurança para os profissionais de Enfermagem, é um serviço de extrema importância e a aplicação rigorosa dessas práticas, como a correta higienização das mãos, o uso de equipamentos de proteção individual e o descarte adequado de materiais perfurocortantes, é vital para proteger tanto os profissionais de saúde quanto os pacientes. A adoção de tais medidas, além de garantir um ambiente seguro, reduz a ocorrência de infecções hospitalares, minimizando complicações e promovendo um atendimento de qualidade ao público. Este estudo visa analisar o papel do enfermeiro nesse contexto, destacando seus desafios e contribuições para tais práticas. Objetivo: Descrever a importância na prática de Biossegurança na rotina de Enfermagem em centro cirúrgico. Material e Métodos: O material utilizado para embasar este presente estudo consiste em revisão narrativa, bibliográfica de literatura, artigos científicos, literatura especializada na área de biossegurança para profissionais de enfermagem. Foram selecionados estudos científicos nas bases de dados SCIELO, Google acadêmico. Resultados e Discussão: Foram selecionados 8 artigos para a criação do estudo, da tabela e para a discussão sobre o trabalho da prática da biossegurança para os profissionais de enfermagem. Conclusão: Diante do exposto neste estudo, compreende-se que a prática de Biossegurança da enfermagem em centro cirúrgico é fundamental para preservar a saúde tanto do paciente quanto dos profissionais de saúde envolvidos e é essencial para promover um ambiente seguro ao público.

Palavras-chave: Biossegurança, Enfermagem e Centro Cirúrgico.
PRÁTICAS PEDAGÓGICAS NO ATENDIMENTO À CRIANÇA COM AUTISMO
Authors: Salvador Alana Paula Lazzarotto , Feitosa Maria Cleide Vieira da Silva , Santos Maria da Glória Ramos dos
Published: November 2024

O relato expõe a vivência na Escola Municipal de Educação Infantil “Jeferson Galbiatti Mira”, diante da presença notável de crianças com Transtorno do Espectro Autista (TEA). Para atendê-las de forma mais abrangente, o projeto "Práticas Pedagógicas no Atendimento à Criança com Autismo" foi implementado no segundo ciclo do Projeto Sala de Formação. Esse projeto teve como propósito enriquecer a experiência das turmas Pré I C, Pré I D e Pré I E, buscando promover a compreensão da singularidade de cada criança. A intenção foi desenvolver valores como autoestima, empatia, respeito e cooperação entre os alunos. Diversas atividades foram conduzidas para estimular habilidades motoras, cognitivas e sociais. Entre elas, o jogo das cores permitiu que as crianças identificassem objetos na sala conforme a cor apresentada, contribuindo para o conhecimento das cores, percepção visual e tátil. Além disso, atividades como utilizar pinça para pegar pompons e fazer pareamentos de cores foram realizadas, visando o desenvolvimento da coordenação motora fina, movimentos precisos e reconhecimento de cores. Outra atividade aplicada foi a seriação de imagens, oportunizando à criança a associação de imagens com as cores correspondentes, praticando a comparação entre elas e identificando as diferenças. Já o circuito das cores consistiu em apresentar quatro sequências em cores distintas, onde as crianças montavam sequências empilhando bolas coloridas sobre copos. Essa atividade promoveu a capacidade de identificar e reproduzir sequências, bem como a percepção visual e tátil. Todas essas experiências geraram atitudes positivas de participação e colaboração entre o grupo, estimulando a compreensão da singularidade de cada indivíduo. Enfatizaram, também, a importância do respeito às diferenças para uma convivência harmônica. O projeto, ao possibilitar o desenvolvimento de habilidades motoras e cognitivas, contribuiu para um ambiente de aprendizado inclusivo e enriquecedor. Além disso, ao promover valores como empatia e respeito à diversidade, ofereceu uma base sólida para a convivência e interação entre as crianças.
Human Performance Augmenting Drugs and Technologies
Authors: R Dinesh Kumar
Published: November 2024

In many sectors, ranging from sports and military operations to professional settings (notably cognitive enhancement); human performance augmentation has been an enduring ambition. The idea behind this movement, known as human enhancement, has evolved over time from simple means of training to the improvement or augmentation of physical ability through recent developments in fields such as pharmaceuticals and implantable devices that can enhance natural abilities. They span from pharmaceutical agents to wearable technologies used for sophisticated self-experimentation, which offer promise but also present ethical, health, and societal risks Performance-Enhancing Drugs are used to enhance the performance of humans in one way or another as a basic idea. The categories of Performance Enhancing Drugs (PEDs) included are Stimulants: Stimulant drugs, including amphetamines and caffeine (in the form of coffee) have been used for thousands of endeavours and physical stamina for decades. There have been many sources of controversy in the sporting world due to use or misuse and side-effectual restrictions. The release of neurotransmitters such as dopamine and allowing muscles to grow big, and fast. Nevertheless, the consumption of anabolic steroids is strictly controlled because it has side effects and complications such as liver damage, hormonal imbalances, and greater aggression. Erythropoietin EPO is a hormone that produces red blood cells, and increased oxygen delivery to muscle. EPO Athletes and even more notably endurance athletes (such as cyclists) have a storied history of abusing this performance-enhancing agent to build stamina. However, the misuse of this drug can be very hazardous, as it causes complications such as blood clotting and heart issues.
Rol de la ubicación traslacional de elementos regulatorios de la transcripción sobre la acción de complejos remodeladores de cromatina dependientes de ATP.
Authors:   , GIDI FAÚNDEZ CRISTIÁN ALFREDO
Published: November 2024

La dinámica de la cromatina juega un rol fundamental en la regulación de genes eucariontes. Entre los principales actores que participan en esta dinámica, se encuentran los complejos remodeladores de cromatina dependientes de ATP. Se han descrito varios elementos que influencian la actividad de estos complejos, como factores de transcripción y determinadas secuencias de ADN con propiedades posicionadoras o excluyentes de nucleosomas. En este estudio proponemos que un factor de importancia en la dinámica de cromatina es el ordenamiento de elementos regulatorios en la cromatina, específicamente la ubicación de sitios de unión para factores de transcripción respecto de cores nucleosomales.
Para estudiar este aspecto utilizamos un sistema experimental in vitro consistente en la medición de la actividad de complejos remodeladores de cromatina de S. cerevisiae (ySWI/SNF y ISW1a) sobre sondas mononucleosomales, en presencia o ausencia de factores de transcripción, cuyos sitios de unión están presentes bajo diferentes ordenamientos en las sondas mononucleosomales.
En estos análisis encontramos que la extensión de ADN entre un core nucleosomal y un sitio de unión para un factor de transcripción que reclute a un complejo remodelador de cromatina afecta la actividad catalítica del complejo. Para el caso específico de ySWI/SNF, extensiones de ADN superiores a 40 pb entre un nucleosoma posicionado y el sitio de unión del factor de transcripción capaz de reclutar al complejo implican una caída significativa en la actividad del mismo. Por otro lado, en el caso del complejo ISW1a se encontró que un factor de transcripción puede impedir la actividad del complejo si su unión se da en el ADN linker hacia el cual el complejo esté movilizando al octámero de histonas.
Nuestros resultados apuntan a la ubicación precisa de sitios para factores de transcripción, relativo a los cores nucleosomales vecinos, como un elemento determinante para la acción de complejos remodeladores de cromatina dependientes de ATP. Así, este ordenamiento y factores dependientes del microambiente celular, como la presencia y abundancia relativa de factores de transcripción y complejos remodeladores de cromatina, se presentan como agentes fundamentales en la dinámica de los paisajes nucleosomales vinculados a la regulación de la expresión de genes en organismos eucariontes.
Cine-Ensayo, un lugar para la Antropología Audiovisual
Authors: Guarderas Ignacio
Published: November 2024

Dentro de la antropología audiovisual se han desarrollado diferentes modos de producción según los diversos marcos teóricos. La idea es buscar un método de escritura audiovisual que corresponda a una determinada forma de pensar la disciplina. Por ejemplo, para una manera positivista se debería realizar un cine de enunciación objetivo. De esta manera, forma y fondo van de la mano tanto para investigar como para divulgar conocimiento antropológico desde la escritura audiovisual.
Lo que pretendemos discutir en esta ponencia es resolver qué tipo de enunciación cinematográfica cabría para una antropología hermenéutica. Según este marco teórico el foco habría que ponerlo en quien ejerce la antropología y no en el hecho cultural mismo. Este sería una suerte de traductor que interpreta a su manera una determinada cultura como si fuera un texto. Por consiguiente, la información no está en la traducción sino en un entramado contextual que el o la traductora-antropóloga debe describir –como dice Geertz– de manera densa.
El método de escritura cinematográfica que defendemos acorde con dicho marco teórico es el cine-ensayo. Basado en los Essais de Montaigne, esta forma enunciativa no pone el centro en el objeto sino en la medida de los ojos de quien mira, es decir, en este caso, en el traductor-cineasta. Resulta muy difícil hacer una sistematización del cine-ensayo, ya que habrá tantas formas como cineastas la practiquen. No obstante, sí se puede esbozar ciertas líneas que nos hagan pensar la relación entre el cine-ensayo y la antropología.
indianidad del Septentrión Mexicano a través del prisma de la Historia y la Antropología Nacional
Authors: Saucedo Sánchez de Tagle Eduardo
Published: November 2024

El artículo introduce una serie de datos y reflexiones en torno al papel que el concepto de indio ha tenido en el contexto de la antropología mexicana y la historia oficial del país. Especialmente, en la coyuntura de finales del siglo xix y principios del xx, donde confluye el nacimiento formal de la antropología y la cristalización de la creación de la identidad nacional mexicana. Destacando el papel predominante del indio como sujeto y objeto de reflexión en la tradición antropológica en México, así como el manejo intencionado que a lo largo de la historia se ha dado en torno a la figura de los pueblos indígenas. Este texto enfatiza la existencia de una tendencia generalizada a excluir de ese universo de reflexión y análisis la historia y el presente de los pueblos indígenas del norte de México, y muestra algunas de las razones que explican este fenómeno.
Characterization of a Multidrug-resistant Escherichia Coli Lytic Bacteriophage Isolate as a Safe Alternative to Synthetic Antibiotics
Authors: Alharbi Najwa , Yahya Reham
Published: November 2024

The control of pathogenic bacteria depends mainly on the use of antibiotics; however, several problems can arise due to the overuse of broad-spectrum antibiotics that destroy not only pathogens, but also natural beneficial microbes in the gut micro-flora. Furthermore, the development of antibiotic resistance poses a major challenge in clinical medicine. Therefore, there is an urgent therapeutic need to develop safe alternative bactericidal or bacteriostatic approaches that will selectively kill only pathogens without disrupting the micro-flora. One such approach is the use of bacteriophages to suppress the growth of bacterial pathogens in humans and other animals. Sewage water can be a rich source of pathogenic bacteria and their lytic phages; thus, we sampled sewage water in this study and successfully isolated an Escherichia coli strain and its lytic phage. We demonstrated that the phage could repress bacterial growth of Salmonella enterica (ATCC 14028), Klebsiella oxytoca (ATCC49131), Shigella sonnei (ATCC 25931), and E. coli (ATCC25922). The isolated phage was stable at room temperature, but survival declined with an increase in temperature to 70 °C, and complete inhibition was observed at temperatures 80 °C. In addition, the phage was stable over a pH range of 6–8, and was completely inactivated at pH 14.
Keywords: bacteriophage, Escherichia coli strain M00057, EC phage, multidrug- resistance
Reference Levels of Plasma D-dimer for Normal Pregnant and Their Association with Pregnancy Complications: A Single-center Retrospective Analysis
Authors: Wang Wenhuan , Dai Qiqi , Pan Liqin , Zhang Hongping , Wang Yeping
Published: November 2024

Background: The objective of this study was to extract maternal plasma D-dimer test results from a large medical records dataset and to establish a reference level for pregnant women in our center. This study aimed to investigate the role and efficacy of D-dimer levels for predicting complications in high-risk pregnant women. Methods: The clinical data of 19,117 women who delivered at Wenzhou People’s Hospital between January 1, 2020, and September 30, 2022, were retrospectively analyzed. A total of 74,401 D-dimer test results were extracted from the laboratory information system. Following data screening and matching, 7692 records of D-dimer test results for healthy pregnant women and 19,005 records for pregnant women with complications were selected. D-dimer reference values were calculated for early pregnancy (1–13 weeks gestation), mid-pregnancy (14–27 weeks gestation), late pregnancy (28–36 weeks gestation), and the end of pregnancy (37–41 weeks gestation). A logistic regression model was used to plot receiver operating characteristic (ROC) and determine cut-off values for predicting pregnancy complications in each phase. Results: The mean D-dimer levels in the healthy pregnancy group were 0.56 mg/L in early pregnancy, 1.08 mg/L in mid-pregnancy, 1.48 mg/L in late pregnancy, and 1.89 mg/L at the end of pregnancy. D-dimer levels were greater in the complication group (which contains 11 common pregnancy disorders, including premature rupture of membranes (PRM), gestational diabetes mellitus (GDM), gestational hypertension (GHP), preeclampsia (PE), placental abnormalities, thyroid dysfunction, hemorrhage, thrombophilia, fetal macrosomia, preterm delivery, and infections) than in the healthy group in all pregnancy phases, with statistically significant differences observed in early, medium, and the end of pregnancy. D-dimer levels showed good diagnostic performance in predicting pregnancy complications in early pregnancy (area under the curve (AUC) = 0.71, cut-off value = 0.87, odds ratio: 7.12, 95% confidence interval (CI): 5.41–9.36, p-value = 0.000). Conclusions: The reference range of D-dimer increases with the progression of gestational phases and demonstrates predictive efficacy for high-risk pregnancy complications during early pregnancy.
Sodium Iodate: Rapid and Clinically Relevant Model of AMD
Authors: Geathers Jasmine S. , Grillo Stephanie L. , Karakoleva Ema , Campbell Gregory P. , Du Yixuan , Chen Han , Barber Alistair J. , Zhao Yuanjun , Sundstrom Jeffrey M.
Published: November 2024

Background: Age-related macular degeneration (AMD) is the most common cause of vision loss in people above the age of 50, affecting approximately 10% of the population worldwide and the incidence is rising. Hyperreflective foci (HRF) are a major predictor of AMD progression. The purpose of this study was to use the sodium iodate mouse model to study HRF formation in retinal degeneration. Methods: Sodium iodate (NaIO3) treated rodents were studied to characterize HRF. 3-month-old male wild-type (WT) C57Bl/6J mice were injected with phosphate-buffered saline (PBS) or varying doses of NaIO3 (15–60 mg/kg). Optical Coherence Tomography (OCT) images were collected at baseline and several days post-NaIO3 injection. Retinal thicknesses were measured using Bioptigen software. Seven days post-injection, eyes were prepared for either transmission electron microscopy (TEM), Hematoxylin & Eosin (H&E), or immunofluorescence. Results: OCT imaging of the mice given higher doses of NaIO3 revealed HRF formation in the neural retina (n = 4). The amount of HRF correlated with the degree of retinal tissue loss. H&E and TEM imaging of the retinas seven days post-NaIO3 injection revealed several pigmented bodies in multiple layers of the retina (n = 3–5). Immunofluorescence revealed that some pigmented bodies were positive for macrophage markers and an epithelial-to-mesenchymal transition marker, while all were retinal pigment epithelium (RPE) 65-negative (n = 4). Conclusions: The data suggest that NaIO3 induces the formation of HRF in the outer retina and their abundance correlates with retinal tissue loss. The experiments in this study highlight NaIO3 as a clinically relevant model of intermediate AMD that can be used to study HRF formation and to discover new treatment targets.
HMGB1 Modulates Macrophage Metabolism and Polarization in Ulcerative Colitis by Inhibiting Cpt1a Expression
Authors: Wang Fenfen , Luo Linfei , Wu Zhengqiang , Wan Lijun , Li Fan , Wen Zhili
Published: November 2024

Background: Macrophage polarization is involved in the development of ulcerative colitis (UC). This study investigated the mechanism by which high mobility group box-1 protein (HMGB1) regulates macrophage polarization through metabolic reprogramming, thereby contributing to the pathogenesis of UC. Methods: Dextran sulfate sodium (DSS) was used to induce colitis in mice. RAW264.7 cells were polarized to M1 or M2 macrophages in vitro by stimulating with lipopolysaccharide (LPS)/interferon-γ (IFN-γ) or Interleukin-4 (IL-4), respectively. Macrophage infiltration and distribution within colon tissue were assessed by immunohistochemistry and flow cytometry. Glycolysis, fatty acid oxidation (FAO), and inflammatory factors were evaluated using relevant reagent kits. Chromatin Immunoprecipitation (ChIP) and luciferase reporter experiments were performed to study the regulation of Carnitine palmitoyltransferase 1A (Cpt1a) promoter transcriptional activity by HMGB1. Results: The mouse UC model showed upregulated HMGB1 and increased macrophage infiltration. Overexpression of HMGB1 promoted M1 macrophage polarization, increased glycolysis, and reduced FAO, whereas knockdown of HMGB1 promoted M2 macrophage polarization, reduced glycolysis, and increased FAO. HMGB1 negatively regulated Cpt1a expression by inhibiting transcription of the Cpt1a promoter. Knockdown of Cpt1a reversed the effects of small interfering RNA targeting HMGB1 (si-HMGB1) on macrophage metabolism and polarization. Administration of adeno-associated virus (AAV)-shHMGB1 in vivo caused a reduction in UC symptoms and inflammation. Conclusions: HMGB1 modulates macrophage metabolism in UC by inhibiting Cpt1a expression, leading to increased M1 polarization. This provides a theoretical basis for the clinical application of HMGB1 inhibitors in the treatment of UC.
Neurophysiology and Molecular Basis of Cognitive Behavioral Therapy for Patients with Insomnia: Implications for Non-Pharmacological Approaches
Authors: Zhang Guimei , Wang Sisi , Ma Ping , Pan Jiyang
Published: November 2024

Cognitive behavioral therapy for insomnia (CBT-I) is a widely used psychological intervention known for its effectiveness in improving insomnia symptoms. However, the neurophysiological mechanisms underlying the cognitive-behavioral treatment of insomnia remain unclear. This narrative review aimed to elucidate the neurophysiological and molecular mechanisms of CBT-I, focusing on the fields of psychology, neurophysiology, neuroendocrinology, immunology, medical microbiology, epigenetics, neuroimaging and brain function. A comprehensive search was conducted using databases including: PubMed, Embase, PsycINFO and Web of Science, with customized search strategies tailored to each database that included controlled vocabulary and alternative synonyms. It revealed that CBT-I may have a beneficial effect on the central nervous system, boost the immune system, upregulate genes involved in interferon and antibody responses, enhance functional connectivity between the hippocampus and frontoparietal areas and increase cortical gray matter thickness. In conclusion, an integrated model is proposed that elucidates the mechanisms of CBT-I and offers a new direction for investigations into its neurophysiological mechanisms.
White Matter Microstructure Alteration in Patients with Drug-Induced Parkinsonism: A Diffusion Tensor Imaging Study with Tract-Based Spatial Statistics
Authors: Sun Ling , He Shijia , Cheng Bo , Shen Yao , Zhao Wenhao , Tu Rong , Zhang Shushan
Published: November 2024

Introduction: This research aimed to investigate the pathophysiological mechanism of how drug-induced parkinsonism (DIP) affects the integrity of the white matter (WM) fiber microstructure as measured by magnetic resonance diffusion tensor image (DTI) fractional anisotropy (FA) and mean diffusivity (MD). Methods: We recruited 17 participants diagnosed with DIP, 20 Parkinson’s disease (PD) patients, and 16 normal controls (NCs) with a similar age, gender, and years of education. Subsequently, all participants underwent DTI magnetic resonance imaging scanning. To analyze the data, we utilized the software packages Functional MRI of the Brain Centre (FMRIB) Diffusion Toolbox (FDT), developed by the FMRIB laboratory at Oxford University, and tract-based spatial statistics (TBSS). Results: The Argentina Hyposmia Rating Scale (AHRS) scores of patients in DIP group were markedly higher than those in PD patients group. Compared with the NC group, the FA values in the genu and body of the corpus callosum (CC), anterior limb of the right internal capsule, bilateral anterior corona radiata, bilateral superior corona radiata, right external capsule, and right superior fronto-occipital fasciculus (could be a part of the anterior internal capsule) were significantly decreased in the DIP group; however, no significant cluster was found in MD. Conclusions: The present study provides novel insights into the alterations in WM microstructure among DIP patients, suggesting that these methodologies have the potential to aid in the early diagnosis and treatment of DIP.
Comparison of the Effects of Long-term Hemodialysis and Peritoneal Dialysis Modalities on Left Ventricular Functions
Authors: Usta Mehmet , Tiryakioğlu Selma Kenar , Ersoy Alparslan , Şensoy Nur Özer , Demir Ömer Furkan , Buyukuysal Mustafa Cagatay
Published: November 2024

Background: Hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) affect left ventricular hemodynamics. This study compared the effect of two treatment modalities, CAPD and HD, on left ventricular systolic and diastolic functions in maintenance dialysis patients. Methods: A total of 47 patients (24 CAPD and 23 HD) undergoing long-term dialysis were included in the study. Left ventricular functions, left ventricular hypertrophy, and left ventricular geometry were evaluated using echocardiography. Results: The mean age of the patients was 58.6 ± 11.2 years. The mean dialysis time was 125.1 ± 35.2 months. When echocardiographic parameters were examined, left ventricular muscle mass, mass index, E/e’ ratios, and global longitudinal strain were significantly higher in the CAPD group. The rates of diastolic dysfunction (66.7% vs. 26.1%) and left ventricular hypertrophy (91.7% vs. 60.9%) were higher in the CAPD group than in the HD group. Dialysis modality CAPD, abnormal global longitudinal strain (GLS), and increased serum calcium were associated with an increased risk of diastolic dysfunction. Conclusions: The study results demonstrated that left ventricle (LV) diastolic dysfunction and deterioration in left ventricular geometry were significantly higher in patients receiving long-term CAPD treatment than for long-term HD treatment.
From Pulmonary Embolism to Chronic Thromboembolic Pulmonary Hypertension: A Pathophysiological Approach
Authors: Shahidi Parham , Mentzel Luise , Blazek Stephan , Sulimov Dmitry , Thiele Holger , Fengler Karl
Published: November 2024

Venous thromboembolism presenting as deep vein thrombosis or pulmonary embolism (PE) remains to be an important cause of mortality and morbidity worldwide. Despite its significance and incidence, compared to many other cardiovascular conditions there are significant gaps in knowledge in many aspects of it, including its pathophysiology. A rare sequela of PE is chronic thromboembolic pulmonary hypertension (CTEPH). This complication has a poor outcome and data is scarce in this field. Many therapeutic approaches are based solely on clinical expertise, which can be explained by the complex and not fully understood pathobiology of this disease. Over the years, many theories have been proposed regarding its genesis. Although generally acute PE is accepted as a trigger for CTEPH, this condition is multifactorial and cannot be explained by recurring PEs only. By reviewing the current evidence, we have demonstrated that thrombus non-resolution in CTEPH is due to multiple mechanisms and predisposing factors including: inflammation, small-vessel disease, impaired angiogenesis, platelet dysfunction, coagulopathies, malignancy, impaired fibrinolysis, genetics and many other components. Based on the current evidence, we aimed to explain the pathophysiology CTEPH, PE and the connection between these two important diseases. Furthermore, we highlight the negative hemodynamic effects of CTEPH and PE on the right ventricle and its role in further exacerbation of these patients.
Exploring the Impact of Niacin Intake on Cardiovascular Outcomes: A Comprehensive Analysis Using NHANES Data (2003–2018)
Authors: Shao Lishi , Zhi Aihua , Li Manning , Zhang Yang , Jiang Shaohui , Zhang Jun , Yang Ke , Yang Enze , Zhu Xiankang , Cheng Yuanou , Sun Yi
Published: November 2024

Background: The relationship between cardiovascular outcomes and niacin consumption levels remains unclear. This study aimed to examine the correlation between niacin intake and the incidence of cardiovascular disease, as well as the mortality rates associated with cardiovascular disease and other causes. Methods: From 2003 to 2018, we continually investigated updated information from the National Health and Nutrition Examination Survey. Based on the quartiles of niacin intake levels, four distinct categories of participants were established: Q1 (<14.646 mg), Q2 (14.646–21.302 mg), Q3 (21.302–30.401 mg), and Q4 (>30.401 mg). Baseline variable differences were assessed employing the Chi-Square and Student's t-tests. A weighted logistic regression with multiple variables was used to determine the association between niacin intake and cardiovascular disease prevalence. Hazard ratios (HRs) and 95% confidence intervals (CIs) for all-cause death and cardiovascular disease were determined utilising hazard regression models. Kaplan–Meier curves were used to compare survival probability between the high and low niacin intake groups, and dose-response linear relationships were evaluated with restricted cubic splines. Results: The cohort analysis included 80,312 participants for the assessment of niacin intake. Comparing the Q1 dataset to the Q4 dataset in the overall population, weighted Cox regression analysis showed a negative association with all-cause mortality (95% CI: 0.71–0.96, HR: 0.82) and mortality owing to cardiovascular disease (95% CI: 0.67–0.96, odds ratio (OR): 0.80). Sex-based subgroup analysis revealed a detrimental correlation between niacin use and overall mortality in females (Q4 cohort: 95% CI: 0.62–0.97, HR: 0.78) but not in males. Additionally, the Q3 (95% CI: 0.59–0.94, HR: 0.75) and Q4 (95% CI: 0.51–0.97, HR: 0.7) groups exhibited a negative association with female cardiovascular disease mortality compared to the Q1 group. Niacin intake was not significantly correlated with prevalence, all-cause mortality, or death from cardiovascular disease in males. Conclusions: Higher niacin consumption was correlated with a decreased risk of cardiovascular disease and death from all causes across the entire study population. Nevertheless, only females, and not males, exhibited a beneficial effect on mortality.