bluuebunny/crossref_metadata_2025 huggingface dataset:
Brief Oxygen Exposure after Traumatic Brain Injury Hastens Recovery and Promotes Adaptive Chronic Endoplasmic Reticulum Stress Responses
Authors: Torrens Jordyn N. , Hetzer Shelby M. , Evanson Nathan K.
Published: June 2023

Traumatic brain injury (TBI) is a major public health concern, particularly in adolescents who have a higher mortality and incidence of visual pathway injury compared to adult patients. Likewise, we have found disparities between adult and adolescent TBI outcomes in rodents. Most interestingly, adolescents suffer a prolonged apneic period immediately post-injury, leading to higher mortality; therefore, we implemented a brief oxygen exposure paradigm to circumvent this increased mortality. Adolescent male mice experienced a closed-head weight-drop TBI and were then exposed to 100% O2 until normal breathing returned or recovered in room air. We followed mice for 7 and 30 days and assessed their optokinetic response; retinal ganglion cell loss; axonal degeneration; glial reactivity; and retinal ER stress protein levels. O2 reduced adolescent mortality by 40%, improved post-injury visual acuity, and reduced axonal degeneration and gliosis in optical projection regions. ER stress protein expression was altered in injured mice, and mice given O2 utilized different ER stress pathways in a time-dependent manner. Finally, O2 exposure may be mediating these ER stress responses through regulation of the redox-sensitive ER folding protein ERO1α, which has been linked to a reduction in the toxic effects of free radicals in other animal models of ER stress.
Metabolomic Profile and Its Correlation with the Plasmatic Levels of Losartan, EXP3174 and Blood Pressure Control in Hypertensive and Chronic Kidney Disease Patients
Authors: Santos Ingrid Souza Reis , Martin-Pastor Manuel , Tavares Júnior Alberto Gomes , Queiroz Kamila Ayres , da Silva Sólon Lílian Grace , Sousa Francisco Fábio Oliveira de
Published: June 2023

Systemic arterial hypertension (SAH) is one of the most prevalent chronic diseases worldwide and, when dysregulated, may cause serious complications. Losartan (LOS) blocks relevant physiological aspects of hypertension, acting mainly on the reduction of peripheral vascular resistance. Complications of hypertension include nephropathy, in which diagnosis is based on the observation of functional or structural renal dysfunction. Therefore, blood pressure control is essential to attenuate the progression of chronic kidney disease (CKD). In this study, 1H NMR metabolomics were used to differentiate hypertensive and chronic renal patients. Plasmatic levels of LOS and EXP3174, obtained by liquid chromatography coupled with mass-mass spectroscopy, were correlated with blood pressure control, biochemical markers and the metabolomic fingerprint of the groups. Some biomarkers have been correlated with key aspects of hypertension and CKD progression. For instance, higher levels of trigonelline, urea and fumaric acid were found as characteristic markers of kidney failure. In the hypertensive group, the urea levels found could indicate the onset of kidney damage when associated with uncontrolled blood pressure. In this sense, the results point to a new approach to identify CKD in early stages and may contribute to improving pharmacotherapy and reducing morbidity and mortality associated with hypertension and CKD.
The Phosphoproteome of the Rd1 Mouse Retina, a Model of Inherited Photoreceptor Degeneration, Changes after Protein Kinase G Inhibition
Authors: Zhou Jiaming , Welinder Charlotte , Ekström Per
Published: June 2023

Retinitis pigmentosa (RP) is a frequent cause of blindness among the working population in industrial countries due to the inheritable death of photoreceptors. Though gene therapy was recently approved for mutations in the RPE65 gene, there is in general no effective treatment presently. Previously, abnormally high levels of cGMP and overactivation of its dependent protein kinase (PKG) have been suggested as causative for the fatal effects on photoreceptors, making it meaningful to explore the cGMP-PKG downstream signaling for more pathological insights and novel therapeutic target development purposes. Here, we manipulated the cGMP-PKG system in degenerating retinas from the rd1 mouse model pharmacologically via adding a PKG inhibitory cGMP-analogue to organotypic retinal explant cultures. A combination of phosphorylated peptide enrichment and mass spectrometry was then applied to study the cGMP-PKG-dependent phosphoproteome. We identified a host of novel potential cGMP-PKG downstream substrates and related kinases using this approach and selected the RAF1 protein, which may act as both a substrate and a kinase, for further validation. This showed that the RAS/RAF1/MAPK/ERK pathway may be involved in retinal degeneration in a yet unclarified mechanism, thus deserving further investigation in the future.
Cartilage-Related Collagens in Osteoarthritis and Rheumatoid Arthritis: From Pathogenesis to Therapeutics
Authors: Ouyang Ziwei , Dong Lei , Yao Feng , Wang Ke , Chen Yong , Li Shufang , Zhou Renpeng , Zhao Yingjie , Hu Wei
Published: June 2023

Collagens serve essential mechanical functions throughout the body, particularly in the connective tissues. In articular cartilage, collagens provide most of the biomechanical properties of the extracellular matrix essential for its function. Collagen plays a very important role in maintaining the mechanical properties of articular cartilage and the stability of the ECM. Noteworthily, many pathogenic factors in the course of osteoarthritis and rheumatoid arthritis, such as mechanical injury, inflammation, and senescence, are involved in the irreversible degradation of collagen, leading to the progressive destruction of cartilage. The degradation of collagen can generate new biochemical markers with the ability to monitor disease progression and facilitate drug development. In addition, collagen can also be used as a biomaterial with excellent properties such as low immunogenicity, biodegradability, biocompatibility, and hydrophilicity. This review not only provides a systematic description of collagen and analyzes the structural characteristics of articular cartilage and the mechanisms of cartilage damage in disease states but also provides a detailed characterization of the biomarkers of collagen production and the role of collagen in cartilage repair, providing ideas and techniques for clinical diagnosis and treatment.
Estradiol Protects Female ApoE KO Mice against Western-Diet-Induced Non-Alcoholic Steatohepatitis
Authors: Araujo Layanne C. C. , Cruz Alessandra G. , Camargo Felipe N. , Sucupira Felipe G. , Moreira Gabriela V. , Matos Sandro L. , Amaral Andressa G. , Murata Gilson Masahiro , Carvalho Carla R. O. , Camporez Joao Paulo
Published: June 2023

The prevalence of non-alcoholic fatty liver disease (NAFLD) and its severe form, non-alcoholic steatohepatitis (NASH), is higher in men than in women of reproductive age, and postmenopausal women are especially susceptible to developing the disease. Aim: we evaluated if female apolipoprotein E (ApoE) KO mice were protected against Western-diet (WD)-induced NASH. Methods: Female ovariectomized (OVX) ApoE KO mice or sham-operated (SHAM) mice were fed either a WD or a regular chow (RC) for 7 weeks. Additionally, OVX mice fed a WD were treated with either estradiol (OVX + E2) or vehicle (OVX). Results: Whole-body fat, plasma glucose, and plasma insulin were increased and associated with increased glucose intolerance in OVX mice fed a WD (OVX + WD). Plasma and hepatic triglycerides, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) hepatic enzymes were also increased in the plasma of OVX + WD group, which was associated with hepatic fibrosis and inflammation. Estradiol replacement in OVX mice reduced body weight, body fat, glycemia, and plasma insulin associated with reduced glucose intolerance. Treatment also reduced hepatic triglycerides, ALT, AST, hepatic fibrosis, and inflammation in OVX mice. Conclusions: These data support the hypothesis that estradiol protects OVX ApoE KO mice from NASH and glucose intolerance.
Parasitoid Wasp Acerophagus papayae: A Promising Solution for the Control of Papaya Mealybug Paracoccus marginatus in Cassava Fields in Vietnam
Authors: Le Khac Hoang , Tran Thi Hoang Dong , Tran Dang Hoa , Nguyen Tuan Dat , Van Doan Cong
Published: June 2023

Cassava is a valuable export commodity crop that is often attacked by pests, causing economic losses for this crop. The papaya mealybug Paracoccus marginatus has become a major pest of cassava in Vietnam. The parasitoid wasp Acerophagus papayae has been demonstrated to be the most efficient parasitoid wasp for controlling P. marginatus in many regions. We observed the occurrence of A. papayae in Vietnam, studied the biological characteristics of A. papayae, and investigated its parasitic activity on P. marginatus. The results showed that A. papayae occurred more frequently than Anagyrus loecki, another known parasitoid of P. marginatus. The lifespan of A. papayae was approximately 16 days. In the absence of hosts, a 50% honey solution was an essential diet to increase the longevity of both female and male of A. papayae. The second instar of P. marginatus was a suitable host stage for parasitism by A. papayae. Female A. papayae laid approximately 60.8 eggs within 17 days, mostly during the first 6 to 7 days. These findings suggest that A. papayae has the potential to control P. marginatus, and could inform the development of more effective pest management strategies for cassava crops in Vietnam and other regions affected by this pest.
Keep Your Eggs Away: Ant Presence Reduces Ceratitis capitata Oviposition Behaviour through Trait-Mediated Indirect Interactions
Authors: Smargiassi Stefania , Masoni Alberto , Frizzi Filippo , Balzani Paride , Desiato Elisa , Benelli Giovanni , Canale Angelo , Santini Giacomo
Published: June 2023

Alternative methods to achieve sustainable agricultural production while reducing the use of chemical pesticides, such as biological control, are increasingly needed. The exploitation of trait-mediated indirect interactions (TMIIs), in which pests modify their behavior in response to some cues (e.g., pheromones and other semiochemicals) to avoid predation risk, may be a possible strategy. In this study, we tested the effect of TMIIs of two Mediterranean ant species, Crematogaster scutellaris and Tapinoma nigerrimum, on the oviposition behaviour of Ceratitis capitata (Diptera: Tephritidae), one of the world’s most economically damaging pests, which attacks fruits. For each ant species, we performed choice experiments using ant-scented and control plums, counting the time spent by medflies on fruits and the number of pupae emerging from them. Results of both ant species tests showed a significantly shorter time spent by ovipositing medflies on ant-exposed plums and a lower number of pupae, when compared to the control group. Our findings highlighted that the semiochemicals released by ants on plums triggered an avoidance behaviour by medfly females, leading to lower oviposition rates. This study contributes to the understanding of indirect ant–pest interactions in Mediterranean agricultural settings and points out the potential of utilising ant-borne semiochemicals in sustainable IPM strategies.
Predictive Factors of Visual Outcome in Treatment-Naïve Diabetic Macular Edema: Preliminary Results from the Clinical Study “FOVEA”
Authors: Serra Rita , Coscas Florence , Boulet Jean François , Cabral Diogo , Tran Thi Ha Chau , Solinas Giuliana , Pinna Antonio , Lupidi Marco , Coscas Gabriel ,   Fovea Study Group
Published: June 2023

Diabetic macular edema (DME) is a common cause of vision impairment in diabetic retinopathy. The aim of this study was to analyze the relationship between visual outcome and anatomic changes detected by traditional multimodal retinal imaging and optical coherence tomography angiography (OCTA) in DME eyes under treatment with Aflibercept. Methods: Sixty-six DME eyes of 62 patients under treatment with intravitreal Aflibercept and with one-year follow-up were enrolled. All participants underwent a full ophthalmic evaluation, including best correct visual acuity (BCVA) measurement, spectral-domain optical coherence tomography, fluorescein angiography and OCTA, both at baseline and final examination. Fractal OCTA analysis of the superficial and deep capillary plexus (SCP and DCP) was performed to estimate vascular perfusion density and lacunarity (LAC). Results: At the final examination, there was a significant improvement in terms of BCVA and central macular thickness (CMT). Furthermore, eyes with CMT <373 µm at baseline reached the higher BCVA at the last follow-up. Eyes with CMT ≥373 µm and DCP LAC <0.41 reached a higher final BCVA, if compared with eyes showing the same CMT but higher initial LAC. Conclusion: A 12-month treatment with intravitreal Aflibercept for DME resulted in significant visual and anatomic improvement. Multimodal retinal imaging, together with fractal OCTA analysis, may provide useful biomarkers, predictive of visual outcome in DME.
Development of a Visuoperceptual Measure for Fiberoptic Endoscopic Evaluation of Swallowing (V-FEES) in Adults with Oropharyngeal Dysphagia: An International Delphi Study
Authors: Cordier Reinie , Speyer Renée , Langmore Susan , Denman Deborah , Swan Katina , Farneti Daniele
Published: June 2023

Visuoperceptual evaluation of fiberoptic endoscopic evaluation of swallowing (FEES) is a commonly used assessment in dysphagia or swallowing disorders. Currently, no international consensus exists regarding which visuoperceptual measures to use for the analysis of FEES recordings. Moreover, existing visuoperceptual FEES measures are limited by poor and incomplete psychometric data, identifying an urgent need for developing a visuoperceptual measure to interpret FEES recordings. Following the COSMIN group’s (COnsensus-based Standards for the selection of health Measurement INstruments) psychometric taxonomy and guidelines, this study aimed to establish the content validity of a new visuoperceptual FEES (V-FEES) measure in adults with oropharyngeal dysphagia. Using the Delphi technique, international consensus was achieved among dysphagia experts across 21 countries, resulting in a new prototype measure for V-FEES, comprising 30 items, 8 function testing items (i.e., specific tasks performed by patients while observing and rating items), and 36 unique operationalisations (i.e., defining items into measurable factors that could be measured empirically using visuoperceptual observation). This study supports good content validity for V-FEES, including participants’ feedback on the relevance, comprehensiveness, and comprehensibility of the included items. Future studies will continue the instrument development process and determine the remaining psychometric properties using both the classic test theory (CTT) and item response theory (IRT) models.
Awake or Sleeping? Maybe Both… A Review of Sleep-Related Dissociative States
Authors: Sodré Maria Eduarda , Wießner Isabel , Irfan Muna , Schenck Carlos H. , Mota-Rolim Sergio A.
Published: June 2023

Recent studies have begun to understand sleep not only as a whole-brain process but also as a complex local phenomenon controlled by specific neurotransmitters that act in different neural networks, which is called “local sleep”. Moreover, the basic states of human consciousness—wakefulness, sleep onset (N1), light sleep (N2), deep sleep (N3), and rapid eye movement (REM) sleep—can concurrently appear, which may result in different sleep-related dissociative states. In this article, we classify these sleep-related dissociative states into physiological, pathological, and altered states of consciousness. Physiological states are daydreaming, lucid dreaming, and false awakenings. Pathological states include sleep paralysis, sleepwalking, and REM sleep behavior disorder. Altered states are hypnosis, anesthesia, and psychedelics. We review the neurophysiology and phenomenology of these sleep-related dissociative states of consciousness and update them with recent studies. We conclude that these sleep-related dissociative states have a significant basic and clinical impact since their study contributes to the understanding of consciousness and the proper treatment of neuropsychiatric diseases.
Factors Associated with Glomerular Yield in Percutaneous Kidney Biopsy
Authors: Torigoe Kenta , Sakamoto Ryosuke , Abe Shinichi , Muta Kumiko , Mukae Hiroshi , Nishino Tomoya
Published: June 2023

Percutaneous kidney biopsy is essential for diagnosing various kidney diseases. However, insufficient glomerular yield leads to misdiagnosis, a critical problem. We retrospectively investigated the risk of insufficient glomerular yield in percutaneous kidney biopsies. We included 236 patients who underwent percutaneous kidney biopsies between April 2017 and September 2020. We retrospectively analyzed the relationship between glomerular yield and patient characteristics. After the biopsy, 31 patients produced insufficient glomerular yields (cases with yielded glomeruli <10). Glomerular yield correlated negatively with hypertension (β = −0.13, p = 0.04), and positively with glomerular density (β = 0.59, p < 0.0001) and the volume of the biopsy core (number of punctures, number of biopsy cores, total length of biopsy core, length of core collected by one puncture, and cortical length). Patients yielding <10 glomeruli had lower glomerular densities (14.4 ± 1.6 vs. 22.9 ± 0.6/cm; p < 0.0001). These results suggest that glomerular density is crucial to glomerular yield. Furthermore, glomerular density was negatively correlated with hypertension, diabetes, and age. Hypertension was independently associated with low glomerular density (β = −0.16, p = 0.02). Thus, the glomerular yield was associated with glomerular density and biopsy core length, and hypertension might be related to glomerular yield via low glomerular density.
Analysis of Nephrolithiasis Treatment in Highest Reference Hospital—Occurrence of Acromegaly in the Study Group
Authors: Ząbkowski Tomasz , Durma Adam Daniel , Grabińska Agnieszka , Michalczyk Łukasz , Saracyn Marek
Published: June 2023

Background: Urolithiasis is one of the most common diseases of the urinary system, the incidence of which is assumed to be up to 100,000 cases per million (10% of the population). The cause of it is dysregulation of renal urine excretion. Acromegaly is a very rare endocrine disorder that causes a somatotropic pituitary adenoma producing higher amounts of growth hormone. It occurs approximately in 80 cases per million (about 0.008% of the population). One of the acromegaly complications may be urolithiasis. Methods: Clinical and laboratory results of 2289 patients hospitalized for nephrolithiasis in the highest reference hospital were retrospectively analyzed, distinguishing a subgroup of patients with acromegaly. Statistical analysis was performed to compare the prevalence of the disease in the analyzed subgroup with the epidemiological results available in up-to-date literature. Results: The distribution of nephrolithiasis treatment was definitely in favor of non-invasive and minimally invasive treatment. The methods used were as follows: ESWL (61.82%), USRL (30.62%), RIRS (4.15%), PCNL (3.1%), and pyelolithotomy (0.31%). Such a distribution limited the potential complications of the procedures while maintaining the high effectiveness of the treatment. Among two thousand two hundred and eighty-nine patients with urolithiasis, two were diagnosed with acromegaly before the nephrological and urological treatment, and seven were diagnosed de novo. Patients with acromegaly required a higher percentage of open surgeries (including nephrectomy) and also had a higher rate of kidney stones recurrence. The concentration of IGF-1 in patients with newly diagnosed acromegaly was similar to those treated with somatostatin analogs (SSA) due to incomplete transsphenoidal pituitary surgery. Conclusions: In the population of patients with urolithiasis requiring hospitalization and interventional treatment compared to the general population, the prevalence of acromegaly was almost 50-fold higher (p = 0.025). Acromegaly itself increases the risk of urolithiasis.
Pearls and Pitfalls of Weaning an Infant with Severe Atopic Dermatitis and Sensitization/Allergy to Food
Authors: Giovannini Mattia , Bolis Marta , Barni Simona , Liccioli Giulia , Sarti Lucrezia , Morelli Susanna , Pontone Matteo , Pessina Benedetta , Tomei Leonardo , Valleriani Claudia , Novembre Elio , Mori Francesca
Published: June 2023

Atopic dermatitis (AD) is a common chronic inflammatory skin disorder in childhood. Skin barrier impairment exposes infants to food allergens, potentially causing sensitization followed by IgE-mediated food allergy. We describe the case of an infant with severe AD in whom several sensitizations to foods are detected, with consequently difficult weaning, and a history of anaphylaxis to cashew nut. Foods for which skin tests were negative were introduced into the infant’s diet. Then, when AD control was managed, oral food challenges (OFCs) for foods to which the patient was sensitized, with the exception of cashew nut, were performed. The simultaneous presence of sensitization toward multiple foods made it difficult to introduce them using classic OFC. Therefore, it was decided to perform the low-dose, gradual controlled OFC. This led to an introduction of sensitized foods into the infant’s diet, with the exception of cashew nut, avoiding allergic reactions. Absolute recommendations on how, when, and where to perform OFCs with allergenic food to which the child with AD is sensitized are lacking so far. In our opinion, OFCs and the subsequent ntroduction of allergenic foods should be individualized, evaluating some factors such as their social and nutritional importance, the patient’s age and clinical phenotype (including the history of anaphylaxis), and the sensitization profile. There is agreement on the fact that the dietary approach in children with moderate-severe AD should no longer include a strict elimination diet. We believe that an early, gradual controlled introduction of all allergenics to identify the amount of food tolerated in the absence of reactions, even if low dose, may improve patients’ and families’ quality of life. However, even if discussing a vast relevant literature, the limitation of our work is that we describe the management of a single patient. Extensive and high-quality research is needed in this field to improve the available evidence in the area.
An Emerging Animal Model for Querying the Role of Whole Genome Duplication in Development, Evolution, and Disease
Authors: Schvarzstein Mara , Alam Fatema , Toure Muhammad , Yanowitz Judith L.
Published: June 2023

Whole genome duplication (WGD) or polyploidization can occur at the cellular, tissue, and organismal levels. At the cellular level, tetraploidization has been proposed as a driver of aneuploidy and genome instability and correlates strongly with cancer progression, metastasis, and the development of drug resistance. WGD is also a key developmental strategy for regulating cell size, metabolism, and cellular function. In specific tissues, WGD is involved in normal development (e.g., organogenesis), tissue homeostasis, wound healing, and regeneration. At the organismal level, WGD propels evolutionary processes such as adaptation, speciation, and crop domestication. An essential strategy to further our understanding of the mechanisms promoting WGD and its effects is to compare isogenic strains that differ only in their ploidy. Caenorhabditis elegans (C. elegans) is emerging as an animal model for these comparisons, in part because relatively stable and fertile tetraploid strains can be produced rapidly from nearly any diploid strain. Here, we review the use of Caenorhabditis polyploids as tools to understand important developmental processes (e.g., sex determination, dosage compensation, and allometric relationships) and cellular processes (e.g., cell cycle regulation and chromosome dynamics during meiosis). We also discuss how the unique characteristics of the C. elegans WGD model will enable significant advances in our understanding of the mechanisms of polyploidization and its role in development and disease.
Reinforcement of Hydrogels with a 3D-Printed Polycaprolactone (PCL) Structure Enhances Cell Numbers and Cartilage ECM Production under Compression
Authors: Alizadeh Sardroud Hamed , Chen Xiongbiao , Eames B. Frank
Published: June 2023

Hydrogels show promise in cartilage tissue engineering (CTE) by supporting chondrocytes and maintaining their phenotype and extracellular matrix (ECM) production. Under prolonged mechanical forces, however, hydrogels can be structurally unstable, leading to cell and ECM loss. Furthermore, long periods of mechanical loading might alter the production of cartilage ECM molecules, including glycosaminoglycans (GAGs) and collagen type 2 (Col2), specifically with the negative effect of stimulating fibrocartilage, typified by collagen type 1 (Col1) secretion. Reinforcing hydrogels with 3D-printed Polycaprolactone (PCL) structures offer a solution to enhance the structural integrity and mechanical response of impregnated chondrocytes. This study aimed to assess the impact of compression duration and PCL reinforcement on the performance of chondrocytes impregnated with hydrogel. Results showed that shorter loading periods did not significantly affect cell numbers and ECM production in 3D-bioprinted hydrogels, but longer periods tended to reduce cell numbers and ECM compared to unloaded conditions. PCL reinforcement enhanced cell numbers under mechanical compression compared to unreinforced hydrogels. However, the reinforced constructs seemed to produce more fibrocartilage-like, Col1-positive ECM. These findings suggest that reinforced hydrogel constructs hold potential for in vivo cartilage regeneration and defect treatment by retaining higher cell numbers and ECM content. To further enhance hyaline cartilage ECM formation, future studies should focus on adjusting the mechanical properties of reinforced constructs and exploring mechanotransduction pathways.
The Spectrum of Extraglandular Manifestations in Primary Sjögren’s Syndrome
Authors: Mihai Ancuta , Caruntu Constantin , Jurcut Ciprian , Blajut Florin Cristian , Casian Mihnea , Opris-Belinski Daniela , Ionescu Ruxandra , Caruntu Ana
Published: June 2023

Extraglandular manifestations (EGMs) in primary Sjogren’s syndrome (pSS) represent the clinical expression of the systemic involvement in this disease. EGMs are characterized by a wide heterogeneity; virtually any organ or system can be affected, with various degrees of dysfunction. The existing gaps of knowledge in this complex domain of extraglandular extension in pSS need to be overcome in order to increase the diagnostic accuracy of EGMs in pSS. The timely identification of EGMs, as early as from subclinical stages, can be facilitated using highly specific biomarkers, thus preventing decompensated disease and severe complications. To date, there is no general consensus on the diagnostic criteria for the wide range of extraglandular involvement in pSS, which associates important underdiagnosing of EGMs, subsequent undertreatment and progression to severe organ dysfunction in these patients. This review article presents the most recent basic and clinical science research conducted to investigate pathogenic mechanisms leading to EGMs in pSS patients. In addition, it presents the current diagnostic and treatment recommendations and the trends for future therapeutic strategies based on personalized treatment, as well as the latest research in the field of diagnostic and prognostic biomarkers for extraglandular involvement in pSS.
Exploiting Smart Meter Water Consumption Measurements for Human Activity Event Recognition
Authors: Wilhelm Sebastian , Kasbauer Jakob , Jakob Dietmar , Elser Benedikt , Ahrens Diane
Published: June 2023

Human activity event recognition (HAER) within a residence is a topic of significant interest in the field of ambient assisted living (AAL). Commonly, various sensors are installed within a residence to enable the monitoring of people. This work presents a new approach for HAER within a residence by (re-)using measurements from commercial smart water meters. Our approach is based on the assumption that changes in water flow within a residence, specifically the transition from no flow to flow above a certain threshold, indicate human activity. Using a separate, labeled evaluation data set from three households that was collected under controlled/laboratory-like conditions, we assess the performance of our HAER method. Our results showed that the approach has a high precision (0.86) and recall (1.00). Within this work, we further recorded a new open data set of water consumption data in 17 German households with a median sample rate of 0.083¯ Hz to demonstrate that water flow data are sufficient to detect activity events within a regular daily routine. Overall, this article demonstrates that smart water meter data can be effectively used for HAER within a residence.
A Tale of Two Continents (and a Few Islands): Ecology and Distribution of Late Pleistocene Sloths
Authors: McDonald H. Gregory
Published: June 2023

Late Pleistocene sloths were widely distributed and present in a diversity of habitats in South, Central, and North America and some Caribbean Islands. Late Pleistocene sloths include 27 genera in four families Megatheriidae, Megalonychidae, Mylodontidae, and Nothrotheriidae. There is no consensus on the number of valid species. Some sloths have wide geographic distributions and are present on multiple continents while others have a much smaller distribution. Our knowledge of the paleoecology and natural history of the different sloths varies greatly depending on their relative abundance. The wide distribution of sloths and adaptations to different habitats results in several “sloth” faunas with different taxonomic compositions. These generalized faunas can be distinguished geographically as Temperate North America (five genera), Southern Mexico and Central America (five genera), Northern South America (two genera), West Coast of South America (four genera), the Andes and Altiplano (four genera), Brazilian Intertropical Region (nine genera), Pampas-Patagonia and the Caribbean Islands (Cuba, Hispaniola, and Puerto Rico, four genera). Some genera may occur in multiple regions but are represented by different species. These regions also have differences in other mammalian taxa, so the sloths are often in ecological competition with different megaherbivores or preyed on by different carnivores.
Tonal Behavior as of Areal and Typological Concerns: Centering on the Sinitic and Kam-Tai Languages in Lingnan
Authors: Liao Hanbo
Published: June 2023

From the perspective of areal linguistics, this paper examines the similarities in tonal behavior between Sinitic and Kam-Tai, the two most populous language groups in Lingnan. By relying on some frameworks for investigating tone systems, i.e., tone-box theories, which largely involve the evolution of tones, the following duplicating patterns and paths of diffusion of areal features are identified. (1) The secondary tonal split conditioned by vowel length on checked syllables, as well as the secondary tonal split of the upper-register tones conditioned by the laryngeal features of initial consonants, both originated in Kam-Tai languages and have diffused into some neighboring Sinitic languages. (2) The pattern of the secondary tonal split of the lower-register tones conditioned by laryngeal features of the initial consonants originated in northern authoritative Sinitic languages and spread widely among different subgroups of Sinitic languages; its diffusion into the Kam-Tai languages is limited to the lexical category of loanwords. (3) The upper-register tones associated with sonorant initials found in Lingnan Sinitic languages are suggested to be of a Kam-Tai origin trait. Further, their underlying areal typological rules are also summarized, concentrating on different upper limits for the possible secondary tonal split in the Sinitic and Kam-Tai languages, which were determined by the historically distinct laryngeal features of the initial consonants of the two language groups.
Comparative Cutting Fluid Study on Optimum Grinding Parameters of Ti-6Al-4V Alloy Using Flood, Minimum Quantity Lubrication (MQL), and Nanofluid MQL (NMQL)
Authors: Taha-Tijerina Jose Jaime , Edinbarough Immanuel A.
Published: June 2023

Titanium alloys have been of paramount interest to the aerospace industry due to their attractive characteristics. However, these alloys are difficult to machine and require grinding post-processes for quality assurance of the products. Conventional grinding takes a long time and uses a flood coolant-lubrication technique, which is not cost effective nor environmentally friendly. Several studies have been performed to prove the viability and benefit of using Minimum Quantity Lubrication (MQL) with vegetable or synthetic-ester fluids. This work aims to find the optimum grinding parameters of creep feed grinding Ti-6Al-4V with a green silicon carbide wheel, using a flood lubrication system with water-soluble synthetic oil, MQL with ester oil, and nano-MQL (NMQL) using alumina-nanopowder homogeneously dispersed within an ester oil. It is concluded that at 0.635 mm and 1.27 mm infeeds, the three lubrication methods performed similarly. At an infeed of 1.905 mm, MQL did not provide desirable quality, though NMQL and flood lubrication performed practically identically. At a cross feed of 0.254 mm, an infeed of 1.27 mm, and a table feed rate of 6.7 m/min, these grinding parameters provide a material removal rate of 2163 mm3/min with a surface roughness across (Ra) of 0.515 µm. These parameters provide the quickest material removal rate while still maintaining industrial quality. This conclusion is based on environmental, economic, and qualitative results.
Methyl 8- and 5-Nitro-1,4-Benzodioxane-2-Carboxylate
Authors: Armano Edoardo , Giraudo Alessandro , Morano Camillo , Pallavicini Marco , Bolchi Cristiano
Published: June 2023

2-Substituted 1,4-benzodioxanes bearing one or more substituents at benzene are important templates in the design and synthesis of a large variety of biologically active compounds. One of the most straightforward synthetic strategies to prepare them in racemic form and with a 2-substituent susceptible to further synthetically useful conversions is the condensation of commercially available methyl 2,3-dibromopropionate with already suitably functionalized catechol. Here, we obtain methyl 8- and 5-nitro-1,4-benzodioxane-2-carboxylate by reaction of methyl 2,3-dibromopropionate with 3-nitrocatechol. After separation, the two positional isomers could be unequivocally identified by HMBC NMR analysis.
Evaluation of a Hydrophobic Coating Agent Based on Cellulose Nanofiber and Alkyl Ketone Dimer
Authors: Jang Nag-Seop , Noh Chi-Hoon , Kim Young-Hwan , Yang Hee-Jun , Lee Hyeon-Gi , Oh HongSeob
Published: June 2023

In this study, we report on the development and testing of hydrophobic coatings using cellulose fibers. The developed hydrophobic coating agent secured hydrophobic performance over 120°. In addition, a pencil hardness test, rapid chloride ion penetration test, and carbonation test were conducted, and it was confirmed that concrete durability could be improved. We believe that this study will promote the research and development of hydrophobic coatings in the future.
Evaluating Some Functional Properties of Surfaces with Partially Regular Microreliefs Formed by Ball-Burnishing
Authors: Dzyura Volodymyr , Maruschak Pavlo , Slavov Stoyan , Dimitrov Diyan , Semehen Volodymyr , Markov Oleksandr
Published: June 2023

In the present work, functional properties of three different types of regular microreliefs, formed by ball burnishing process on flat surfaces are evaluated. For their estimation, heights and material ratio curve’s criteria were used, according to the international standard ISO 21920-2:2021. The influence of the regular relief’s type and ball burnishing process parameters on the surface functional properties were investigated using full factors experimental design. Based on the obtained results, statistical models were derived that describe the dependencies between the topography characteristics and the regular reliefs’ types and regime parameters of the ball burnishing process. Based on the obtained results, several conclusions have been formulated about the applicability of these standardized methods and criteria for evaluating the functional characteristics of the different types of regular reliefs at the end of the work.
Combined m-Consecutive-k-Out-of-n: F and Consecutive kc-Out-of-n: F Structures with Cold Standby Redundancy
Authors: Triantafyllou Ioannis S.
Published: June 2023

In the present work, we study the combined m-consecutive-k-out-of-n: F and kc-out-of-n: F reliability systems, which consist of independent and identically distributed components. Two different redundancy policies are considered, and their general frameworks are described and illustrated. The main objective of the paper refers to the investigation of the effect of adding cold standby redundancy to the system at the the system level and the component level. Exact formulae for determining the crucial characteristics of the enhanced structure, such as its survival function, the mean time to failure and the mean residual lifetime, are provided. All formulae proved in the present manuscript are explicit expressions which are based on the signature vector of the resulting reliability schemes. An extensive numerical investigation is carried out to shed light on the performance of the combined m-consecutive-k-out-of-n: F and consecutive kc-out-of-n: F reliability systems with cold standby redundancy. Some concluding remarks and comments are provided upon the determination of the optimal design parameters.
A Matching-Strategy-Inspired Preconditioning for Elliptic Optimal Control Problems
Authors: Wang Chaojie , Chen Jie , Sun Shuen
Published: June 2023

In this paper, a new preconditioning method is proposed for the linear system arising from the elliptic optimal control problem. It is based on row permutations of the linear system and approximations of the corresponding Schur complement inspired by the matching strategy. The eigenvalue bounds of the preconditioned matrices are shown to be independent of mesh size and regularization parameter. Numerical results illustrate the efficiency of the proposed preconditioning methods.
Predicting Post-Hepatectomy Liver Failure in HCC Patients: A Review of Liver Function Assessment Based on Laboratory Tests Scores
Authors: Morandi Alessio , Risaliti Matteo , Montori Michele , Buccianti Simone , Bartolini Ilenia , Moraldi Luca
Published: June 2023

The assessment of liver function is crucial in predicting the risk of post-hepatectomy liver failure (PHLF) in patients undergoing liver resection, especially in cases of hepatocellular carcinoma (HCC) which is often associated with cirrhosis. There are currently no standardized criteria for predicting the risk of PHLF. Blood tests are often the first- and least invasive expensive method for assessing hepatic function. The Child–Pugh score (CP score) and the Model for End Stage Liver Disease (MELD) score are widely used tools for predicting PHLF, but they have some limitations. The CP score does not consider renal function, and the evaluation of ascites and encephalopathy is subjective. The MELD score can accurately predict outcomes in cirrhotic patients, but its predictive capabilities diminish in non-cirrhotic patients. The albumin–bilirubin score (ALBI) is based on serum bilirubin and albumin levels and allows the most accurate prediction of PHLF for HCC patients. However, this score does not consider liver cirrhosis or portal hypertension. To overcome this limitation, researchers suggest combining the ALBI score with platelet count, a surrogate marker of portal hypertension, into the platelet–albumin–bilirubin (PALBI) grade. Non-invasive markers of fibrosis, such as FIB-4 and APRI, are also available for predicting PHLF but they focus only on cirrhosis related aspects and are potentially incomplete in assessing the global liver function. To improve the predictive power of the PHLF of these models, it has been proposed to combine them into a new score, such as the ALBI-APRI score. In conclusion, blood test scores may be combined to achieve a better predictive value of PHLF. However, even if combined, they may not be sufficient to evaluate liver function and to predict PHLF; thus, the inclusion of dynamic and imaging tests such as liver volumetry and ICG r15 may be helpful to potentially improve the predictive capacity of these models.
Corrosion and Protection in Aeronautical Alloys
Authors: Almeraya-Calderón Facundo , Chacon-Nava Jose
Published: June 2023

Corrosion in the aircraft industry continues to be a major problem that directly affects safety, costs, and aircraft availability [...]
Medicinal Plants, Phytochemicals and Regulation of the NLRP3 Inflammasome in Inflammatory Bowel Diseases: A Comprehensive Review
Authors: Direito Rosa , Barbalho Sandra Maria , Figueira Maria Eduardo , Minniti Giulia , de Carvalho Gabriel Magno , de Oliveira Zanuso Bárbara , de Oliveira dos Santos Ana Rita , de Góes Corrêa Natália , Rodrigues Victória Dogani , de Alvares Goulart Ricardo , Guiguer Elen Landgraf , Araújo Adriano Cressoni , Bosso Henrique , Fornari Laurindo Lucas
Published: June 2023

Ongoing research explores the underlying causes of ulcerative colitis and Crohn’s disease. Many experts suggest that dysbiosis in the gut microbiota and genetic, immunological, and environmental factors play significant roles. The term “microbiota” pertains to the collective community of microorganisms, including bacteria, viruses, and fungi, that reside within the gastrointestinal tract, with a particular emphasis on the colon. When there is an imbalance or disruption in the composition of the gut microbiota, it is referred to as dysbiosis. Dysbiosis can trigger inflammation in the intestinal cells and disrupt the innate immune system, leading to oxidative stress, redox signaling, electrophilic stress, and inflammation. The Nod-like Receptor (NLR) Family Pyrin Domain Containing 3 (NLRP3) inflammasome, a key regulator found in immunological and epithelial cells, is crucial in inducing inflammatory diseases, promoting immune responses to the gut microbiota, and regulating the integrity of the intestinal epithelium. Its downstream effectors include caspase-1 and interleukin (IL)-1β. The present study investigated the therapeutic potential of 13 medicinal plants, such as Litsea cubeba, Artemisia anomala, Piper nigrum, Morus macroura, and Agrimonia pilosa, and 29 phytocompounds such as artemisitene, morroniside, protopine, ferulic acid, quercetin, picroside II, and hydroxytyrosol on in vitro and in vivo models of inflammatory bowel diseases (IBD), with a focus on their effects on the NLRP3 inflammasome. The observed effects of these treatments included reductions in IL-1β, tumor necrosis factor-alpha, IL-6, interferon-gamma, and caspase levels, and increased expression of antioxidant enzymes, IL-4, and IL-10, as well as regulation of gut microbiota. These effects could potentially provide substantial advantages in treating IBD with few or no adverse effects as caused by synthetic anti-inflammatory and immunomodulated drugs. However, additional research is necessary to validate these findings clinically and to develop effective treatments that can benefit individuals who suffer from these diseases.
Thermal Energy Harvesting from Slow Variations in Environmental Temperatures
Authors: Curry Joshua , Harris Nick , White Neil
Published: June 2023

With the Internet of Things expanding to more locations across our planet, power becomes the main factor affecting device longevity. There is a need for more novel energy harvesting systems that are able to power remote devices for sustained periods. This publication presents one such device. Based on a novel actuator that utilises off-the-shelf gas mixtures to generate a variable force from temperature change, this publication presents a device capable of generating up to 150mJ per diurnal temperature cycle; this is enough electrical energy to send up to three LoRaWAN transmissions per day using slowly changing environmental temperatures.
Using SMART Magnetic Fluids and Gels for Prevention and Destruction of Bacterial Biofilms
Authors: Król Jarosƚaw E. , Ehrlich Garth D.
Published: June 2023

Biofouling is a major problem in all natural and artificial settings where solid surfaces meet liquids in the presence of living microorganisms. Microbes attach to the surface and form a multidimensional slime that protects them from unfavorable environments. These structures, known as biofilms, are detrimental and very hard to remove. Here, we used SMART magnetic fluids [ferrofluids (FFs), magnetorheological fluids (MRFs), and ferrogels (FGs) containing iron oxide nano/microparticles] and magnetic fields to remove bacterial biofilms from culture tubes, glass slides, multiwell plates, flow cells, and catheters. We compared the ability of different SMART fluids to remove biofilms and found that commercially available, as well as homemade, FFs, MRFs, and FGs can successfully remove biofilm more efficiently than traditional mechanical methods, especially from textured surfaces. In tested conditions, SMARTFs reduced bacterial biofilms by five orders of magnitude. The ability to remove biofilm increased with the amount of magnetic particles; therefore, MRFs, FG, and homemade FFs with high amounts of iron oxide were the most efficient. We showed also that SMART fluid deposition can protect a surface from bacterial attachment and biofilm formation. Possible applications of these technologies are discussed.
Assessment of Saturation Effect on Hydraulic Fracturing in Sandstone and Thermally Treated Granite
Authors: Shevtsova Anna , Stanchits Sergey , Filev Egor , Karamov Tagir , Stukachev Vladimir , Spasennykh Mikhail
Published: June 2023

In this study, a set of laboratory experiments was carried out to study the parameters of hydraulic fractures induced in the dry and mineral-oil-saturated rocks and compare them with the geomechanical characteristics of tested samples. We chose sandstone and thermally treated granite as the materials for research. There are very few known studies related to the mechanical and acoustic properties of oil-saturated rocks, and even fewer studies describing, in detail, the parameters of hydraulic fractures generated in oil-saturated rocks. The hydraulic fracture parameters were determined using a set of independent sensors installed to measure the axial deformation of the sample (which is directly related to the aperture of created hydraulic fracture), fluid pressure, fluid volume injected into hydraulic fracture, and localization of acoustic emission (AE) events, generated during the propagation of hydraulic fractures. Our study focuses on the investigation of the influence of rock properties, altered by mineral oil saturation and thermal treatment, on such parameters of hydraulic fracturing as breakdown pressure (BP), fracture aperture, and the resulting roughness of the hydraulic fracture surface. In addition, we studied the influence of injected fluid viscosity on the parameters of created hydraulic fractures. It was revealed that the saturation state caused a reduction in the values of mechanical parameters such as Young’s modulus, compressive strength, and cohesion, and had a similar reducing influence on the breakdown pressure. The values of HF parameters, such as fracture width and the volume of fracturing agent injected into the HF, are higher in the tests for both saturated sandstone and saturated TT granite. However, we found out that thermal treatment of granite samples led to a much more significant reduction in the values of mechanical and acoustic parameters than the mineral-oil saturation procedure because it created a dense network of thermally induced cracks. The results obtained in our laboratory studies can be taken into account in the modeling of hydraulic fracturing in the field.