bluuebunny/crossref_metadata_2025 huggingface dataset:
Green Demand Aware Fog Computing: A Prediction-Based Dynamic Resource Provisioning Approach
Authors: Pg. Ali Kumar Dk. Siti Nur Khadhijah , Newaz S. H. Shah , Rahman Fatin Hamadah , Lee Gyu Myoung , Karmakar Gour , Au Thien-Wan
Published: February 2022

Fog computing could potentially cause the next paradigm shift by extending cloud services to the edge of the network, bringing resources closer to the end-user. With its close proximity to end-users and its distributed nature, fog computing can significantly reduce latency. With the appearance of more and more latency-stringent applications, in the near future, we will witness an unprecedented amount of demand for fog computing. Undoubtedly, this will lead to an increase in the energy footprint of the network edge and access segments. To reduce energy consumption in fog computing without compromising performance, in this paper we propose the Green-Demand-Aware Fog Computing (GDAFC) solution. Our solution uses a prediction technique to identify the working fog nodes (nodes serve when request arrives), standby fog nodes (nodes take over when the computational capacity of the working fog nodes is no longer sufficient), and idle fog nodes in a fog computing infrastructure. Additionally, it assigns an appropriate sleep interval for the fog nodes, taking into account the delay requirement of the applications. Results obtained based on the mathematical formulation show that our solution can save energy up to 65% without deteriorating the delay requirement performance.
Cryptographic Services Based on Elementary and Chaotic Cellular Automata
Authors: Corona-Bermúdez Erendira , Chimal-Eguía Juan Carlos , Téllez-Castillo Germán
Published: February 2022

In this paper, a security framework based on cellular automata is proposed, which is composed of three parts: entity authentication, data encryption, and decryption. Authentication is based on a zero-knowledge protocol, and its robustness is supported by the fact that the shared secret is not only an NP-complete problem but also dynamically transformed by a two-dimensional cellular automaton into a more complex secret. Thus, the authentication’s output is the key to data encryption/decryption in the following steps. One more strength of this system is the sensibility of the cellular automata to initial conditions—which is why we must know the initial configuration in order to obtain the key and the times that cellular automata evolved. All those characteristics give the system both robustness and security.
A Review of Environmental and Economic Implications of Closing the Nuclear Fuel Cycle—Part One: Wastes and Environmental Impacts
Authors: Taylor Robin , Bodel William , Stamford Laurence , Butler Gregg
Published: February 2022

Globally, around half a million tonnes of spent nuclear fuel (SNF) will be in dry or wet storage by around 2050. Continued storage is not sustainable, and this SNF must eventually either be disposed (the open nuclear fuel cycle) or recycled (the closed fuel cycle). Many international studies have addressed the advantages and disadvantages of these options. To inform this debate, a detailed survey of the available literature related to environmental assessments of closed and open cycles has been undertaken. Environmental impacts are one of the three pillars that, alongside economic and societal impacts, must be considered for sustainable development. The aims are to provide a critical review of the open literature in order to determine what generic conclusions can be drawn from the broad base of international studies. This review covers the results of life cycle assessments and studies on waste arisings, showing how the management of spent fuels in the open and closed cycles impact the environment, including the use of natural resources, radioactive waste characteristics (heat loading, radiotoxicity and volume) and the size of the geological repository. In the framework of sustainable development, the next part of this review will consider economic impacts.
Experimental Study on Tight Sandstone Reservoir Gas Permeability Improvement Using Electric Heating
Authors: Chen Ze , Li Gao , Yang Xu , Zhang Yi
Published: February 2022

Although tight sandstone gas formations are abundant in China, their single-well productivities and exploitation efficiencies are restricted by water blocking from drilling and completion. At present, shut-in, chemical additive application, and hydraulic fracturing are the common approaches applied to handle this problem. However, these approaches are also characterized by low efficiencies or even cause secondary damage. In this study, the impact of high temperatures (of up to 800 °C) on the microstructure of a tight sandstone, including water blocking and gas permeability, are investigated through the electric heating of a simulated wellbore. The results show that the threshold temperature for fracturing of the tight sandstone is approximately 450 to 600 °C. Many secondary microcracks emerged near the wellbore beyond this temperature, improving the gas permeability, with some microcracks visible even after cooling. The gas permeability of the formation after heating to 800 °C increased by 456% and 3992% compared with the initial gas permeability and the water-blocking impacted gas permeability, respectively. This study demonstrates that electric heating is a potential method for improving the permeability of tight gas formations.
Forest Dendromass as Energy Feedstock: Diversity of Properties and Composition Depending on Systematic Genus and Organ
Authors: Stolarski Mariusz Jerzy , Dudziec Paweł , Olba-Zięty Ewelina , Stachowicz Paweł , Krzyżaniak Michał
Published: February 2022

Exhaustion of fossil fuel resources, shrinking forest areas, with accompanying deterioration of their quality and striving (also of the society) to make forests perform their ecological function, with simultaneous development and propagation of the biomass conversion technologies—all of this necessitates research of forest biomass diversification. It is a consequence of the fact that its properties and composition depend not only on the genus but also on the plant organ, and they each time determine its usability as a raw biomaterial in a wide range of thermal, physical, or chemical conversion processes. This study reviewed and analysed selected qualitative and quantitative features of forest dendromass, taking into account the genus and a plant organ/morphological part, followed by a group of trees (coniferous and deciduous) and without the latter differentiation. The study involved an analysis of data covering 15 selected qualitative-quantitative features of forest dendromass within three main and nine additional plant organs/morphological parts and 21 genera (5 coniferous and 16 deciduous) typical of the temperate climate.
Intelligent Power Distribution Restoration Based on a Multi-Objective Bacterial Foraging Optimization Algorithm
Authors: Moraes Carlos Henrique Valério de , Vilas Boas Jonas Lopes de , Lambert-Torres Germano , Andrade Gilberto Capistrano Cunha de , Costa Claudio Inácio de Almeida
Published: February 2022

The importance of power in society is indisputable. Virtually all economic activities depend on electricity. The electric power systems are complex, and move studies in different areas are motivated to make them more efficient and solve their operational problems. The smart grids emerged from this approach and aimed to improve the current systems and integrate electric power using alternative and renewable sources. Restoration techniques of these networks are being developed to reduce the impacts caused by the usual power supply interruptions due to failures in the distribution networks. This paper presents the development and evaluation of the performance of a multi-objective version of the Bacterial Foraging Optimization Algorithm for finding the minor handling switches that maximize the number of buses served, keeping the configuration radial system and within the limits of current in the conductors and bus voltage. An electrical system model was created, and routines were implemented for the network verification, which was used as a function of the Multi-Objective Bacterial Foraging Optimization Hybrid Algorithm. The proposed method has been applied in two distribution systems with 70 buses and 201 buses, respectively, and the algorithm’s effectiveness to solve the restoration problem is discussed.
District Heating of Buildings by Renewable Energy Using Thermochemical Heat Transmission
Authors: Critoph Robert E. , Pacho Angeles M. Rivero
Published: February 2022

The decarbonisation of building heating in urban areas can be achieved by heat pumps connected to district heating networks. These could be ‘third-generation’ (85/75 °C), ‘fourth-generation’ (50/40 or 50/25 °C) or ‘fifth-generation’ (near ambient) water loops. Networks using thermochemical reactions should require smaller pipe diameters than water systems and be more economic. This work investigates thermochemical transmission systems based on liquid–gas absorption intended for application in urban district heating networks where the main heat source might be a MW scale heat pump. Previous studies of absorption for heat transmission have concentrated on long distance (e.g., 50 km) transmission of heat or cold utilizing waste heat from power stations or similar but these are not directly applicable to our application which has not been investigated before. Absorbent-refrigerant pairs are modelled using water, methanol and acetone as absorbates. Thermodynamic properties are obtained from the literature and modelling carried out using thermodynamic analysis very similar to that employed for absorption heat pumps or chillers. The pairs with the best performance (efficiency and power density) both for ambient loop (fifth-generation) and high temperature (fourth-generation) networks use water pairs. The next best pairs use methanol as a refrigerant. Methanol has the advantage of being usable at ambient temperatures below 0 °C. Of the water-based pairs, water–NaOH is good for ambient temperature loops, reducing pipe size by 75%. Specifically, in an ambient loop, heat losses are typically less than 5% and the heat transferred per volume of pumped fluid can be 30 times that of a pumped water network with 10 K temperature change. For high temperature networks the heat losses can reach 30% and the power density is 4 times that of water. The limitation with water–NaOH is the low evaporating temperature when ambient air is the heat source. Other water pairs perform better but use lithium compounds which are prohibitively expensive. For high temperature networks, a few water- and methanol-based pairs may be used, but their performance is lower and may be unattractive.
Investigating the Optimization Design of Internal Flow Fields Using a Selective Catalytic Reduction Device and Computational Fluid Dynamics
Authors: Hu Bin , Chen Cong , Jiang Shouxi , Liu Xiaosong , Dai Qianjin
Published: February 2022

Selective catalytic reduction (SCR) and denitrification are the best technologies for nitrogen oxides (NOx) control in coal-fired power plants, and their denitration efficiency and ammonia escape rate are closely related to their internal flow characteristics. By adding a deflector to the SCR device, the flow field in the curve can be effectively improved, and the stable and efficient operation of the SCR device can be realized. Based on the numerical simulation method, the SCR system of a coking coal-fired boiler in a steel plant was simulated using k-ε (the turbulence model), and three design schemes of deflectors were proposed and numerically simulated simultaneously. After optimization, the ammonia injection grid’s downstream velocity variance coefficient CV was 6.69, the catalyst upper cross-section velocity variance coefficient was 11.84, the cross-sectional temperature average was 499 K, the maximum temperature deviation was 9 °C, the maximum-to-minimum temperature interval span was 15 °C, the cross-sectional NH3/NOx molar ratio average value was 0.8122, the coefficient of variance was 4.67, and the pressure loss was 1855 Pa. The findings of this work will help improve the denitration efficiency and provide an important reference for the actual transformation design.
Understanding the Access to Fuels and Technologies for Cooking in Peru
Authors: Ramírez-Candia Judith , Curt Maria Dolores , Domínguez Javier
Published: February 2022

There is global concern regarding access to energy, especially in developing countries, as set forth in the Sustainable Development Goals. Although Peru is classified as an emerging economy and would be expected to have achieved full energy coverage, the status of the access to fuels in Peru is unknown. The objective of this study was to comprehensively document the instruments and the progress made on the issue of access to modern fuels and technologies for cooking in Peru to explain the current situation and to highlight the main challenges that the country must face to achieve total access to modern energy sources. A comprehensive literature review was carried out for this work, covering a wide range of publications from 1983 to 2019. A total of 18 political and economic instruments and 95 voluntary instruments were analyzed. It made it possible to build a historical series of the main events leading to access to modern cooking fuels in Peru and to identify eight key challenges. The results show that the country has made remarkable progress in recent years, but this progress is not enough to close the access gap. Therefore, seems advisable to act on the current policy framework, formulate more inclusive policies, promote unified institutional efforts and generate technological options that respond to territory and population as diverse as Peru.
A Summary Review on Experimental Studies for PCM Building Applications: Towards Advanced Modular Prototype
Authors: Faraj Khaireldin , Khaled Mahmoud , Faraj Jalal , Hachem Farouk , Castelain Cathy
Published: February 2022

The use of phase change material (PCM) as a thermal energy storage system integrated in new buildings, and as retrofits in old buildings, proved its merit as promising renewable energy source. Heating and cooling building applications of PCM have been studied by plenty of scientists, globally, on the basis of numerical and experimental analysis. The performed experiments have mainly focused on one application—active or passive—using permanent prototype design for a certain duration and weather condition. The current study is a new review that focuses on two complementary aspects: (1) Reviewing latest studies in PCM domain and assessing the thermal performance of implemented prototypes within the conducted PCM experimental studies, namely, the effect of the design on the number of acceptable variables and possible PCM applications; and (2) suggesting a new, advanced, flexible, and modular prototype designed to enable several applications of PCM to be adapted and combined within the model. It was implied that the new design allows different configurations that are lacking in the literature and serves for future PCM building applications of thermal analysis towards PCM integration optimization, as an attempt for transforming residential compartments into net zero energy buildings. The designed prototype overcomes the deficiencies found in previous reviewed experimental facilities.
The Effect of Robinia pseudoacacia Plantation on Soil Desiccation across Different Precipitation Zones of the Loess Plateau, China
Authors: Liang Haibin , Meng Zhilong , Li Zongshan , Liu Guohua
Published: February 2022

Ecological restoration has increased vegetation cover and reduced soil erosion, but it has also resulted in decreased soil-moisture content (SMC) and increased soil desiccation, which has ultimately led to a weakening of the “soil reservoir” function and a decline in the growth of plantations. Thus, soil desiccation has been a serious threat to the sustainable utilization of soil water resources and vegetation rehabilitation. In this study, the soil moisture of a Robinia pseudoacacia forest as well as its corresponding soil desiccation to a depth of 500 cm were measured across three different precipitation zones (400–450, 500–550 and 550–600 mm) along a north–south transect on the Loess Plateau. The results showed that the soil-moisture environment and soil desiccation status generally improved with the increasing precipitation gradient, while soil-moisture over-consumption significantly declined (p < 0.05). However, due to the elder forest-stand age and severe growth recession, the soil desiccation of R. pseudoacacia in the northern part was less than that in central zones. As the forest-stand age increased, SMC of R. pseudoacacia increased firstly and then decreased, and both soil-moisture consumption and the average soil desiccation rate peaked in the RP-5a, showing no significant consistence with forest-stand age. Therefore, understanding the soil-moisture status of forestland may better provide scientific basis for native vegetation restoration and reconstruction in water-limited regions.
Experimental Characterization of Particulate and Gaseous Emissions from Biomass Burning of Six Mediterranean Species and Litter
Authors: Nestola Enrica , Sgrigna Gregorio , Pallozzi Emanuele , Caccavale Loredana , Guidolotti Gabriele , Calfapietra Carlo
Published: February 2022

Wildfires across the Mediterranean ecosystems are associated with safety concerns due to their emissions. The type of biomass determines the composition of particulate matter (PM) and gaseous compounds emitted during the fire event. This study investigated simulated fire events and analysed biomass samples of six Mediterranean species and litter in a combustion chamber. The main aims are the characterization of PM realized through scanning electron microscopy (SEM/EDX), the quantification of gaseous emissions through gas chromatography (GC-MS) and, consequently, identification of the species that are potentially more dangerous. For PM, three size fractions were considered (PM10, 2.5 and 1), and their chemical composition was used for particle source-apportionment. For gaseous components, the CO, CO2, benzene, toluene and xylene (BTXs) emitted were quantified. All samples were described and compared based on their peculiar particulate and gaseous emissions. The primary results show that (a) Acacia saligna was noticeable for the highest number of particles emitted and remarkable values of KCl; (b) tree species were related to the fine windblown particles as canopies intercept PM10 and reemit it during burning; (c) shrub species were related to the particles resuspended from soil; and (d) benzene and toluene were the dominant aromatic compounds emitted. Finally, the most dangerous species identified during burning were Acacia saligna, for the highest number of particles emitted, and Pistacia lentiscus for its high density of particles, the presence of anthropogenic markers, and the highest emissions of all gaseous compounds.
Comparative Investigations on Different β-Glucosidase Surrogate Substrates
Authors: Graf Felix Marcel Rainer , Buchhaupt Markus
Published: February 2022

β-glucosidases are hydrolyzing enzymes which can release many aroma-active compounds from their glycoside form. Several yeasts produce these enzymes and thus are applied during the wine production process. To be able to test specific organisms for the presence of β-glucosidases and to investigate this enzyme activity, four main surrogate substrates have been described. The properties and applicability of these compounds, named arbutin (hydroquinone-β-D-glucopyranoside), esculin (6-O-(-D-glucosyl)aesculetin), 4-nitrophenyl-β-D-glucopyranoside (pNPG) and 4-methylumbelliferyl-β-D-glucopyranoside (4-MUG), are discussed after comparing their advantages and disadvantages. Although all four substrates were found suitable for photometric assays, 4-MUG has proven to be most appropriate due to high sensitivity, high robustness and simple processing. Furthermore, the investigation of β-glucosidase product accumulation is described, which could be used to give indications about β-glucosidase localization.
Past Logging and Wildfire Increase above Ground Carbon Stock Losses from Subsequent Wildfire
Authors: Wilson Nicholas , Bradstock Ross
Published: February 2022

Background: Wildfire is known to reduce forest carbon stocks, but the influence of antecedent disturbance on wildfire related carbon stock losses is not as well understood. Disturbances such as logging and wildfire may increase the vulnerability of remaining carbon stocks to subsequent wildfire. Conversely, these disturbances may reduce the impact of subsequent wildfire, resulting in lower carbon stock losses. Methods: We measured above ground carbon stocks in productive resprouting Eucalypt dominated forests before and after a mixed severity fire that burned during the 2019/20 ‘Black Summer’ fire season in south-eastern Australia. The initial surveys were stratified by time since logging and time since wildfire, allowing for an assessment of how these disturbance histories influence above ground carbon stock losses caused by subsequent wildfire. Results: Above ground carbon stock losses varied substantially; however, there was a weak decrease in losses associated with time since logging but not time since wildfire. Variance in carbon stock losses associated with logging were greater than that caused by the severity of the 2019/20 wildfire itself. Carbon losses and predicted effects of disturbance may be underestimated in some cases due to the accumulation of carbon at sites between pre- and post-fire surveys. Conclusions: This study presents the largest published dataset of direct carbon stock changes resulting from wildfire in eucalypt forests. Our findings indicate that logging reduces the stability of above ground carbon stocks in resprouting eucalypt forests. This information will be critical for land managers looking to manage forests for carbon sequestration.
Effects of Modified Atmosphere Packaging with Various CO2 Concentrations on the Bacterial Community and Shelf-Life of Smoked Chicken Legs
Authors: Wang Qiang , Chen Qian , Xu Jianhang , Sun Fangda , Liu Haotian , Kong Baohua
Published: February 2022

The effects of modified atmosphere packaging (MAP) with various CO2 concentrations on the bacterial community and shelf-life of smoked chicken legs during 25 d of storage at 4 °C were evaluated herein. Four treatments were stored in pallets (PAL) and MAP under 20% (M20), 60% (M60), and 100% (M100) CO2, respectively. The results indicated that the MAP treatments provided the legs with higher redness and hardness and lower yellowness, luminance, and lipid oxidation, compared with the PAL treatment. In addition, the MAP treatments effectively inhibited the growth of viable bacteria, delayed bacterial spoilage, and extended the shelf-life of the samples. The M60 and M100 treatments had a better inhibition effect on bacteria. In terms of bacterial community, Carnobacterium, Pseudomonas, Brochothrix, and Lactococcus were the most predominant genera in the 25 d-stored MAP samples, with Carnobacterium maltaromaticum, Pseudomonas fragi, Shewanella baltica, and Lactococcus piscium being the dominant species. However, while the inhibition effects of the M60 and M100 treatments on the bacterial community at Day 25 were similar, the outer package of the M100 treatment collapsed. Overall, the M60 treatment may be a promising approach to improving the quality and extending the shelf-life of smoked chicken legs.
Macroalgal Proteins: A Review
Authors: O' Brien Ronan O’ , Hayes Maria , Sheldrake Gary , Tiwari Brijesh , Walsh Pamela
Published: February 2022

Population growth is the driving change in the search for new, alternative sources of protein. Macroalgae (otherwise known as seaweeds) do not compete with other food sources for space and resources as they can be sustainably cultivated without the need for arable land. Macroalgae are significantly rich in protein and amino acid content compared to other plant-derived proteins. Herein, physical and chemical protein extraction methods as well as novel techniques including enzyme hydrolysis, microwave-assisted extraction and ultrasound sonication are discussed as strategies for protein extraction with this resource. The generation of high-value, economically important ingredients such as bioactive peptides is explored as well as the application of macroalgal proteins in human foods and animal feed. These bioactive peptides that have been shown to inhibit enzymes such as renin, angiotensin-I-converting enzyme (ACE-1), cyclooxygenases (COX), α-amylase and α-glucosidase associated with hypertensive, diabetic, and inflammation-related activities are explored. This paper discusses the significant uses of seaweeds, which range from utilising their anthelmintic and anti-methane properties in feed additives, to food techno-functional ingredients in the formulation of human foods such as ice creams, to utilising their health beneficial ingredients to reduce high blood pressure and prevent inflammation. This information was collated following a review of 206 publications on the use of seaweeds as foods and feeds and processing methods to extract seaweed proteins.
Identification of Benzophenone Analogs in Rice Cereal through Fast Pesticide Extraction and Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry
Authors: Liu Xuan-Rui , Huang Yu-Fang , Huang Jun-Jie
Published: February 2022

A fast, robust, and sensitive analytical method was developed and validated for the simultaneous identification of benzophenone (BP) and nine BP analogs (BP-1, BP-2, BP-3, BP-8, 2-hydroxybenzophenone, 4-hydroxybenzophenone, 4-methylbenzophenone [4-MBP], methyl-2-benzoylbenzoate, and 4-benzoylbiphenyl) in 25 samples of rice cereal. Fast pesticide extraction (FaPEx) coupled with ultrahigh-performance liquid chromatography–tandem mass spectrometry was applied. The developed method exhibited satisfactory linearity (r > 0.997), favorable recoveries between 71% and 119%, and a limit of detection ranging from 0.001 to 0.5 ng/g. The detection frequencies of BP, 4-MBP, and BP-3 were 100%, 88%, and 52%, respectively. BP had higher geometric levels, with a mean of 39.8 (19.1–108.9) ng/g, and 4-MBP had low levels, with a mean of 1.9 (1.3–3.3) ng/g. The method can be applied to routine rice cereal analysis at the nanogram-per-gram level. For infants aged 0–3 years, the hazard quotients of BP and 4-MBP were lower than one, and the margin of exposure for BP was higher than 10,000, suggesting that rice cereal consumption poses no health concern for Taiwanese infants.
Heat-Killed Enterococcus faecalis EF-2001 Attenuate Lipid Accumulation in Diet-Induced Obese (DIO) Mice by Activating AMPK Signaling in Liver
Authors: Fan Meiqi , Choi Young-Jin , Wedamulla Nishala Erandi , Tang Yujiao , Han Kwon Il , Hwang Ji-Young , Kim Eun-Kyung
Published: February 2022

To explore the inhibitory mechanism of heat-killed Enterococcus faecalis, EF-2001 on hepatic lipid deposition, a diet-induced obese (DIO) animal model was established by high-fat diet (HFD). The DIO C57BL/6 mice were divided into four groups: the normal group without HFD (ND, n = 8), obesity group (HFD, n = 8), experimental group (HFD + EF-2001, 200 mg/kg, n = 8), and positive control group (HFD + Orlistat, 60 mg/kg, n = 8). After 4 weeks, liver and adipose tissue were fixed in 10% paraformaldehyde, followed by embedding in paraffin for tissue sectioning. The differences in body mass, body fat ratio, fatty cell area, and lipid profiling of the liver (TC, LDL, and HDL) were also determined. Moreover, Western blot was performed to analyze the expression of lipid accumulation-related proteins, including AMPK, PPARγ, SREBP-1, ACC, and FAS. Compared with the HFD group, the HFD + EF-2001 group exhibited decreased fat mass, liver index, adipocyte area, TC, and LDL, and an increased level of HDL. The results of liver hematoxylin and eosin (H&E), and oil red O staining showed that the mice in each intervention group were improved on hepatic lipid accumulation, and the mice in the HFD + EF-2001 group were the most similar to those in the normal group when compared with the HFD group. From the Western blot results, we proved that EF-2001 activated the AMPK signaling pathway. EF-2001 significantly upregulated the expressions of p-AMPK and p-ACC and downregulated PPARγ, SREBP-1, and FAS in murine liver. Taken together, these results suggest that EF-2001 decrease lipid accumulation in the DIO model mice through the AMPK pathway and ameliorate liver damage by HFD.
Latent Membrane Protein 1 (LMP1) from Epstein–Barr Virus (EBV) Strains M81 and B95.8 Modulate miRNA Expression When Expressed in Immortalized Human Nasopharyngeal Cells
Authors: Müller Coan Barbara G. , Cesarman Ethel , Acencio Marcio Luis , Elgui de Oliveira Deilson
Published: February 2022

The Epstein–Barr virus (EBV) is a ubiquitous γ herpesvirus strongly associated with nasopharyngeal carcinomas, and the viral oncogenicity in part relies on cellular effects of the viral latent membrane protein 1 (LMP1). It was previously described that EBV strains B95.8 and M81 differ in cell tropism and the activation of the lytic cycle. Nonetheless, it is unknown whether LMP1 from these strains have different effects when expressed in nasopharyngeal cells. Thus, herein we evaluated the effects of EBV LMP1 derived from viral strains B95.8 and M81 and expressed in immortalized nasopharyngeal cells NP69SV40T in the regulation of 91 selected cellular miRNAs. We found that cells expressing either LMP1 behave similarly in terms of NF-kB activation and cell migration. Nonetheless, the miRs 100-5p, 192-5p, and 574-3p were expressed at higher levels in cells expressing LMP1 B95.8 compared to M81. Additionally, results generated by in silico pathway enrichment analysis indicated that LMP1 M81 distinctly regulate genes involved in cell cycle (i.e., RB1), mRNA processing (i.e., NUP50), and mitochondrial biogenesis (i.e., ATF2). In conclusion, LMP1 M81 was found to distinctively regulate miRs 100-5p, 192-5p, and 574-3p, and the in silico analysis provided valuable clues to dissect the molecular effects of EBV LMP1 expressed in nasopharyngeal cells.
Cognitive, Behavioral, and Sensory Profile of Pallister–Killian Syndrome: A Prospective Study of 22 Individuals
Authors: Fetta Anna , Soliani Luca , Trevisan Alessia , Pugliano Rosa , Ricci Emilia , Di Pisa Veronica , Pignataro Veronica , Angotti Marida , Rocca Alessandro , Salce Bianca , Mancardi Maria Margherita , Giordano Lucio , Pruna Dario , Parmeggiani Antonia , Cordelli Duccio Maria
Published: February 2022

Background: Developmental delay and intellectual disability are two pivotal elements of the phenotype of Pallister–Killian Syndrome (PKS). Our study aims to define the cognitive, adaptive, behavioral, and sensory profile of these patients and to evaluate possible correlations between the different aspects investigated and with the main clinical and demographic variables. Methods: Individuals of any age with genetically confirmed PKS were recruited. Those ≤ 42 months were administered the Bayley Scales of Infant and Toddler Development Third Edition (Bayley-III), and those > 42 months the Vineland Adaptive Behavior Scales—Second Edition (Vineland-II). Stereotyped behaviors (Stereotypy Severity Scale, SSS) and aggressive behaviors (Behavior Problems Inventory—Short Version, BPIs) were assessed in all subjects > 1 year; sensory profile (Child Sensory Profile 2, C-SP2) in all aged 2–18 years. Results: Twenty-two subjects were enrolled (11 F/11 M; age 9 months to 28 years). All subjects ≤ 42 months had psychomotor developmental delay. Of the subjects > 42 months, 15 had low IQ deviation, and 1 in the normal range. Stereotypies were frequent (median SSS-total score 25/68). Lower Vineland-II values corresponded to greater intensity and frequency of stereotypies (p = 0.004 and p = 0.003), and self-injurious behaviors (p = 0.002 and p = 0.002). Patients with severe low vision had greater interference of stereotypies (p = 0.027), and frequency and severity of aggressive behaviors (p = 0.026; p = 0.032). The C-SP2, while not homogeneous across subjects, showed prevalence of low registration and sensory seeking profiles and hypersensitivity to tactile and auditory stimuli. Lower Vineland-II scores correlated with higher Registration scores (p = 0.041), while stereotypies were more frequent and severe in case of high auditory sensitivity (p = 0.019; p = 0.007). Finally, greater sleep impairment correlated with stereotypies and self-injurious behaviors, and lower Vineland-II scores. Conclusions: The present study provides a further step in the investigation of the etiopathogenesis of the syndrome. Furthermore, these aspects could guide rehabilitation therapy through the identification of targeted protocols.
An Approach for the Automatic Characterization of Underwater Dunes in Fluviomarine Context
Authors: Cassol Willian Ney , Daniel Sylvie , Guilbert Éric
Published: February 2022

The identification of underwater landforms represents an important role in the study of the seafloor morphology. In this context, the segmentation and characterization of underwater dunes allow a better understanding of the dynamism of the seafloor, since the formation of these structures is directly related to environmental conditions, such as current, tide, grain size, etc. In addition, it helps to ensure safe navigation, especially in the context of navigation channels requiring periodic maintenance. This paper proposes a novel method to automatically characterize the underwater dunes. Its originality relies on the extraction of morphological descriptors not only related to the dune itself, but also to the fields where the dunes are located. Furthermore, the proposed approach involves the entire surface of the dunes, rather than profiles or group of pixels as generally found in previous works. Considering the surface modelled by a digital bathymetric model (DBM), the salient features of the dunes (i.e., crest line, stoss trough, and lee trough) are first identified using a geomorphometric analysis of the DBM. The individual dunes are built by matching the crest lines with their respective troughs according to an object-oriented approach. Then, a series of morphological descriptors, selected through a literature review, are computed by taking advantage of the dune salient features, surface representation, and spatial distribution in the fields where they are located. The validation of the proposed method has been conducted using more than 1200 dunes in the fluvio-marine context of the Northern Traverse of the Saint Lawrence River.
Prevalence of Coronavirus Disease 2019 (COVID-19) in Different Clinical Stages before the National COVID-19 Vaccination Programme in Malaysia: A Systematic Review and Meta-Analysis
Authors: Ng Jun Wei , Chong Eric Tzyy Jiann , Tan Yee Ann , Lee Heng Gee , Chan Lan Lan , Lee Qin Zhi , Saw Yen Tsen , Wong Yiko , Zakaria Ahmad Aizudeen Bin , Amin Zarina Binti , Lee Ping-Chin
Published: February 2022

More than 1.75 million COVID-19 infections and 16 thousand associated deaths have been reported in Malaysia. A meta-analysis on the prevalence of COVID-19 in different clinical stages before the National COVID-19 Vaccination Program in Malaysia is still lacking. To address this, the disease severity of a total of 215 admitted COVID-19 patients was initially recorded in the early phase of this study, and the data were later pooled into a meta-analysis with the aim of providing insight into the prevalence of COVID-19 in 5 different clinical stages during the outset of the COVID-19 pandemic in Malaysia. We have conducted a systematic literature search using PubMed, Web of Science, Scopus, ScienceDirect, and two preprint databases (bioRxiv and medRxiv) for relevant studies with specified inclusion and exclusion criteria. The quality assessment for the included studies was performed using the Newcastle–Ottawa Scale. The heterogeneity was examined with an I2 index and a Q-test. Funnel plots and Egger’s tests were performed to determine publication bias in this meta-analysis. Overall, 5 studies with 6375 patients were included, and the pooled prevalence rates in this meta-analysis were calculated using a random-effect model. The highest prevalence of COVID-19 in Malaysia was observed in Stage 2 cases (32.0%), followed by Stage 1 (27.8%), Stage 3 (17.1%), Stage 4 (7.6%), and Stage 5 (3.4%). About two-thirds of the number of cases have at least one morbidity, with the highest percentage of hypertension (66.7%), obesity (55.5%), or diabetes mellitus (33.3%) in Stage 5 patients. In conclusion, this meta-analysis suggested a high prevalence of COVID-19 occurred in Stage 2. The prevalence rate in Stage 5 appeared to be the lowest among COVID-19 patients before implementing the vaccination program in Malaysia. These meta-analysis data are critically useful for designing screening and vaccination programs and improving disease management in the country.
Use of the Natural Outdoor Environment in Different Populations in Europe in Relation to Access: Implications for Policy
Authors: Masterson Daniel , Triguero-Mas Margarita , Marquez Sandra , Zijlema Wilma , Martinez David , Gidlow Christopher , Smith Graham , Hurst Gemma , Cirach Marta , Grazuleviciene Regina , Van den Berg Magdalena , Kruize Hanneke , Maas Jolanda , Nieuwenhuijsen Mark
Published: February 2022

This cross-cultural study explores the relationship of natural outdoor environment (NOE) use with NOE access. Most urban planning recommendations suggest optimal accessibility to be 300 m–500 m straight distance to spaces with vegetation of at least 1 hectare. Exploring this recommendation, we used data (n = 3947) from four European cities collected in the framework of the PHENOTYPE study: Barcelona (Spain), Doetinchem (The Netherlands), Kaunas (Lithuania) and Stoke-on-Trent (United Kingdom) to obtain residential access to NOE (straight or network distances, using 300 m and 150 m buffers, to NOE larger than 1 hectare or 0.5 hectare) and use of NOE (i.e., self-reported time spent in NOE). Poisson regression models were used to examine the associations between residential access and use of NOE. The models with the strongest association with time spent in NOE in the combined sample were for those living within 300 m straight line distance to either 0.5 ha or 1 ha NOE. Noting that the only indicator that was consistent across all individual cities was living with 150 m network buffer of NOE (of at least 1 ha), this warrants further exploration in reducing recommendations of 300 m straight-line distance to 150 m network distance to 1 ha of NOE for a general indicator for cities within Europe.
Physical Fitness and Inflammatory Response to the Training Load of Wheelchair Rugby Players
Authors: Szymczak Łukasz , Podgórski Tomasz , Lewandowski Jacek , Janiak Arkadiusz , Michalak Edyta , Domaszewska Katarzyna
Published: February 2022

The aim of the study was the evaluation of the hormonal response of wheelchair rugby participants under the half-year training cycle. The study sample included 11 members of the Polish national wheelchair rugby team with spinal cord injury at the cervical level, ranging in age from 21 to 41 years, body weight (72.2 ± 11.53 kg), and body height (182.3 ± 6.11 cm). The disabled individuals with spinal cord injury subjected to the study constitute a homogeneous group in terms of age, body height, weight, and injury level. The study was carried out at the beginning and at the end of a 6-month training period. In the first and second examination, measurements of the peak oxygen uptake (peakVO2) and blood biochemical analysis were performed (Lactate dehydrogenase (LDH) activity and concentration of creatinine (Cr), total testosterone (TT), free testosterone (FT), and cortisol (C)). A significant change was observed in the concentration of C in the Wheelchair Rugby players’ blood between two research periods (p < 0.05 (ES:0.76)) and a correlation between the post-training change in FT/C concentration and the change in Cr concentration (r = −0.6014, p < 0.05). The 6-month training period did not result in overloads within the group of players. However, due to the significant loss of the capacity of the spinal cord injury (SCI) and the possibility of a life-threatening trend, the anabolic/catabolic status of the players should be monitored using blood biochemical indices.
Burnout Syndrome in a Military Tertiary Hospital Staff during the COVID-19 Contingency
Authors: Martínez-Cuazitl Adriana , Martínez-Salazar Iván Noé , Maza-De La Torre Guadalupe , García-Dávila Jorge Arturo , Montelongo-Mercado Edgardo Alonso , García-Ruíz Antonio , Noyola-Villalobos Héctor Faustino , García-Araiza Mayra Gabriela , Hernández-Díaz Sergio , Villegas-Tapia Dora Luz , Cerda-Reyes Eira , Chávez-Velasco Arleth Sarai , García-Hernández Juan Salvador
Published: February 2022

(1) Background: Burnout syndrome (BOS) is defined as a psychological state of physical and mental fatigue associated with work. The COVID-19 pandemic greatly impacted the physical and mental wellbeing of health professionals. The objective of this work was to determine the impact on personnel, monitoring the frequency of BOS throughout the pandemic. (2) Methods: The Maslach Burnout Inventory (MBI) was self-applied in four periods of the pandemic according to sociodemographic and employment characteristics. In this study, all hospital personnel were included; the association of BOS with sex, age, type of participant (civilian or military), military rank and profession was analyzed. (3) Results: The frequency of BOS was 2.4% (start of the pandemic), 7.9% (peak of the first wave), 3.7% (end of the first wave) and 3.6% (peak of the third wave). Emotional exhaustion (EE) was the most affected factor, and the groups most affected were men under 30 years of age, civilians, chiefs and doctors, especially undergraduate medical doctors and specialty resident doctors, and nursing personnel were less affected. (4) Conclusions: The low BOS levels show that the containment measures and military training implemented by the hospital authorities were effective, although the chief personnel were more affected in the first wave. It is probable that this combination allowed the containment of BOS, which was not observed in civilians.
Factors Influencing Users’ Perceived Restoration While Using Treetop Trails: The Case of the Fu and Jinjishan Forest Trails, Fuzhou, China
Authors: Wu Yuanjing , Li Qianyun , Zheng Hanqing , Luo Shijie , Liu Qunyue , Yan Zhao , Huang Qitang
Published: February 2022

Studies have indicated that urban greenways promote physical and perceived restoration. However, there is a lack of research on the impact of treetop trails on human perceived restoration. In this study, two representative treetop trails in Fuzhou city were selected to investigate treetop trails’ impact on users’ perceived restoration. The study adopted a structural equation modelling approach to explore the influence mechanisms and pathways of treetop trails on users’ perceived restoration, through 412 questionnaires. The results showed that the perceived environmental quality of treetop trails had a significant positive effect on users’ overall psychological wellbeing. Place attachment had a significant positive effect on users’ perceived restoration and a significant mediating effect on users’ perceived environmental quality of trails. The results of this study revealed that the mechanisms of the impact of treetop trails on users’ perceived restoration and the construction of treetop trails can be enhanced in the future by improving trail facilities, enriching trail perception of elevated feeling, improving trail landscape quality, and optimising trail design.
How Long Does Adaption Last for? An Update on the Psychological Impact of the Confinement in Portugal
Authors: Costa Ana Daniela , Fernandes Afonso , Ferreira Sónia , Couto Beatriz , Machado-Sousa Mafalda , Moreira Pedro , Morgado Pedro , Picó-Pérez Maria
Published: February 2022

During the first COVID-19 related confinement in Portugal, there was a decrease in the levels of psychological symptoms measured by the Depression, Anxiety and Stress Scale 21 (March to April 2020). Upon experiencing a new period of restraints in 2021, the psychological impact of this sample was assessed again (N = 322, two more time points). It was expected that the psychological symptoms evidenced in February 2021 would be at similar levels to those found in April 2020, leading to a transfer of adaptation. Contrary to our hypothesis, in the second confinement in Portugal there were higher levels of depression and stress symptoms than at the beginning of the pandemic. On the other hand, the maximum level of anxiety was observed in March 2020. It seems that our perception of the threats in 2021 was not the same as at the onset of COVID-19, or that knowledge was not disseminated to the general population to increase their mental health literacy and help them cope with the imposed challenges.
Healthy Dwelling: Design of Biophilic Interior Environments Fostering Self-Care Practices for People Living with Migraines, Chronic Pain, and Depression
Authors: Huntsman Dorothy Day , Bulaj Grzegorz
Published: February 2022

The benefits of biophilic interior design have been recognized by healthcare facilities, but residential environments receive relatively less attention with respect to improving the health of people living with chronic diseases. Recent “stay-at-home” restrictions due to the COVID-19 pandemic further emphasized the importance of creating interior spaces that directly and indirectly support physical and mental health. In this viewpoint article, we discuss opportunities for combining biophilic interventions with interior design, fostering disease-specific self-care. We provide examples of designing residential spaces integrating biophilic interventions, light therapy, relaxation opportunities, mindfulness meditation, listening to music, physical activities, aromatherapy, and quality sleep. These modalities can provide the clinical benefits of reducing migraine headaches and chronic pain, as well as improving depressive symptoms. The disease-specific interior environment can be incorporated into residential homes, workplaces, assisted-living residences, hospitals and hospital at home programs. This work aims to promote a cross-disciplinary dialogue towards combining biophilic design and advances in lifestyle medicine to create therapeutic interior environments and to improve healthcare outcomes.
Determining the Profile of People with Fall Risk in Community-Living Older People in Algarve Region: A Cross-Sectional, Population-Based Study
Authors: Guerreiro Carla , Botelho Marta , Fernández-Martínez Elia , Marreiros Ana , Pais Sandra
Published: February 2022

One in three people aged 65 years or older falls every year. Injuries associated with this event among the older population are a major cause of pain, disability, loss of functional autonomy and institutionalization. This study aimed to assess mobility and fall risk (FR) in community-living older people and to determine reliable and independent measures (health, social, environmental and risk factors) that can predict the mobility loss and FR. In total, 192 participants were included, with a mean age of 77.93 ± 8.38. FR was assessed by EASY-Care (EC) Standard 2010, the Tinetti Test and the Modified Falls Efficacy Scale (MFES). An exploratory analysis was conducted using the divisive non-hierarchical cluster method, aiming to identify a differentiator and homogeneous group of subjects (optimal group of variables) and to verify if that group shows differences in fall risk. Individually, the health, social, environmental and risk factor categories were not found to be an optimal group; they do not predict FR. The most significant predictor variables were a mix of the different categories, namely, the presence of pain, osteoarthritis (OA), and female gender. The finding of a profile that allows health professionals to be able to quickly identify people at FR will enable a reduction in injuries and fractures resulting from falls and, consequently, the associated costs.