bluuebunny/crossref_metadata_2025 huggingface dataset:
Bacillus velezensis BER1 enriched Flavobacterium daejeonense-like bacterium in the rhizosphere of tomato against bacterial wilt
Authors: Wang Ning , Ding Jia , Chen Yanting , Zhu Yuelin , Zhang Lina , Wei Yuquan , Li Ji , Xu Ting , Ding Guo-chun
Published: May 2023

Beneficial microorganisms can protect crop from phytopathogens, and modify rhizosphere microbiome. However, it is not well-understood whether or how do rhizosphere microorganisms which respond to bioagents contribute to disease suppression. Bacillus velezensis BER1 and tomato bacterial wilt caused by Ralstonia solanacearum were selected as models to disentangle the interactions and mechanisms in the rhizosphere. Bacillus velezensis BER1 greatly suppressed tomato bacterial wilt by over 49.0%, reduced R. solanacearum colonization in the rhizosphere by 36.3%, and significantly enriched two Flavobacterium ASVs (1357 and 2401). A novel colony loop-mediated isothermal amplification (LAMP) assay system was developed to screen out Flavobacterium from tomato rhizosphere bacterial isolates. In vitro tests revealed that cocultivating BER1 with Flavobacterium C45 increased biofilm formation by 18.6%. Climate chamber experiment further revealed that Flavobacterium C45 improved the control efficiency of BER1 on tomato bacterial wilt by 46.0%, decreased the colonization of R. solanacearum in the rhizosphere by 43.1% and elevated the transcription of plant defense gene PR1α in tomato by 45.4%. In summary, Flavobacterium C45 boosted the ability of B. velezensis BER1 to prevent bacterial wilt and the colonization of R. solanacearum, highlighting the importance of helper bacteria on elevating the efficiency of biological control.
Seed Storage, Dormancy, and Germination Behavior in Lithocarpus glaber (Fagaceae)
Authors: Jaganathan Ganesh K
Published: May 2023

Knowledge of seed storage behavior and germination ecology of Fagaceae species is limited to the Quercus genus, prompting the need to investigate other genera. Using Lithocarpus glaber (Thunb.) Nakai acorns collected from China, storage behavior, dormancy type, and germination pattern were investigated by germination and desiccation experiments along with triphenyl tetrazolium chloride (TTC) staining and photomicrographs. The fairly large-sized acorns with a mass of 1,340 g per 1,000 acorns had a moisture content of 34.5% (fresh weight) at the time of dispersal. There were three distinctive regions: (1) scar (hilum), (2) apex, and (3) pericarp, with an embryonic axis located on the apex side. When tested for germination at 15/20°C, 76.6% of the acorns germinated within the first 3 weeks, indicating nondormancy. However, germination declined to 48% and 3% when dried in silica gel to 26% and 17% moisture content, respectively, confirming the acorns are recalcitrant. Germination was hypogeal. Nonetheless, the elongated cotyledonary petiole establishes an intumescent tubular structure at approximately 7–10 cm from acorns, from which taproots and leaves emerge. The nondormant recalcitrant acorns took 6–8 weeks to complete the germination process (from imbibition to leaf emergence), possibly indicating the presence of epicotyl dormancy. This is the first study on Lithocarpus showing intumescent tubular structure development during germination. The ecological significance of this structure requires further investigation.
A High Frequency of Chromosomal Duplications in Unicellular Algae Is Compensated by Translational Regulation
Authors: Krasovec Marc , Merret Rémy , Sanchez Frédéric , Sanchez-Brosseau Sophie , Piganeau Gwenaël
Published: May 2023

Although duplications have long been recognized as a fundamental process driving major evolutionary innovations, direct estimates of spontaneous chromosome duplication rates, leading to aneuploid karyotypes, are scarce. Here, from mutation accumulation (MA) experiments, we provide the first estimates of spontaneous chromosome duplication rates in six unicellular eukaryotic species, which range from 1 × 10−4 to 1 × 10−3 per genome per generation. Although this is ∼5 to ∼60 times less frequent than spontaneous point mutations per genome, chromosome duplication events can affect 1–7% of the total genome size. In duplicated chromosomes, mRNA levels reflected gene copy numbers, but the level of translation estimated by polysome profiling revealed that dosage compensation must be occurring. In particular, one duplicated chromosome showed a 2.1-fold increase of mRNA but translation rates were decreased to 0.7-fold. Altogether, our results support previous observations of chromosome-dependent dosage compensation effects, providing evidence that compensation occurs during translation. We hypothesize that an unknown posttranscriptional mechanism modulates the translation of hundreds of transcripts from genes located on duplicated regions in eukaryotes.
Identifying Potential Causal Effects of Telomere Length on Health Outcomes: A Phenome-Wide Investigation and Mendelian Randomization Study
Authors: Wang Wenxiu , Huang Ninghao , Zhuang Zhenhuang , Song Zimin , Li Yueying , Dong Xue , Xiao Wendi , Zhao Yimin , Jia Jinzhu , Liu Zhonghua , Qi Lu , Huang Tao
Published: May 2023

Background

Telomere length has been linked to various health outcomes. To comprehensively investigate the causal effects of telomere length throughout the human disease spectrum, we conducted a phenome-wide Mendelian randomization study (MR-PheWAS) and a systematic review of MR studies.

Methods

We conducted a PheWAS to screen for associations between telomere length and 1 035 phenotypes in the UK Biobank (n = 408 354). The exposure of interest was the genetic risk score (GRS) of telomere length. Observed associations passing multiple testing corrections were assessed for causality by 2-sample MR analysis. A systematic review of MR studies on telomere length was performed to harmonize the published evidence and complement our findings.

Results

Of the 1 035 phenotypes tested, PheWAS identified 29 and 78 associations of telomere length GRS at a Bonferroni- and false discovery rate-corrected threshold; 24 and 66 distinct health outcomes were causal in the following principal MR analysis. The replication MR using data from the FinnGen study provided evidence of causal effects of genetically instrumented telomere length on 28 out of 66 outcomes, including decreased risks of 5 diseases in respiratory diseases, digestive diseases, and myocardial infarction, and increased risks of 23 diseases, mainly comprised neoplasms, diseases of the genitourinary system, and essential hypertension. A systematic review of 53 MR studies found evidence to support 16 out of the 66 outcomes.

Conclusions

This large-scale MR-PheWAS identified a wide range of health outcomes that were possibly affected by telomere length, and suggested that susceptibility to telomere length may vary across disease categories.
Depressive Symptoms, Glial Fibrillary Acid Protein Concentrations, and Cognitive Decline in a Cohort Study
Authors: Desai Pankaja , Krueger Kristin R , Mendes de Leon Carlos , Wilson Robert S , Evans Denis A , Rajan Kumar B
Published: May 2023

Background

Little is known about how depressive symptoms and glial fibrillary acid protein (GFAP) concentrations taken together may influence cognitive functioning. Understanding this relationship may inform strategies for screening and early intervention to decrease the rate of cognitive decline.

Methods

This study sample includes 1 169 participants from the Chicago Health and Aging Project (CHAP), consisting of 60% Black participants and 40% White participants, and 63% female participants and 37% male participants. CHAP is a population-based cohort study of older adults with a mean age of 77 years. Linear mixed-effects regression models tested the main effects of depressive symptoms and GFAP concentrations and their interactions on baseline cognitive function and cognitive decline over time. Models included adjustments for age, race, sex, education, chronic medical conditions, body mass index, smoking status, alcohol use, and their interactions with time.

Results

The interaction of depressive symptomology and GFAP (β = −0.105 [standard error = 0.038], p = .006) on global cognitive function was statistically significant. Participants with depressive symptoms including and above the cutoff and high log of GFAP concentrations had more cognitive decline over time, followed by participants with depressive symptoms below the cutoff and high log of GFAP concentrations, depressive symptom scores including and above the cutoff and low log of GFAP concentrations, and depressive symptom scores below the cutoff and low log of GFAP concentrations.

Conclusions

Depressive symptoms have an additive effect on the association between the log of GFAP and baseline global cognitive function.
Digital Gender Gap in the Second Half of Life Is Declining: Changes in Gendered Internet Use Between 2014 and 2021 in Germany
Authors: Bünning Mareike , Schlomann Anna , Memmer Nicole , Tesch-Römer Clemens , Wahl Hans-Werner
Published: May 2023

Objectives

The main purpose of the study has been to examine changes in Internet use among men and women in 3 age groups (midlife, early old age, and advanced old age) between 2014 and 2021. We tested 2 hypotheses: The complementary hypothesis posits that online activities reproduce gender differences in offline activities. The compensatory hypothesis posits that women are catching up over time in male-typed activities as Internet access approaches saturation for both genders.

Methods

We used representative, longitudinal data from the German Ageing Survey collected in 2014, 2017, 2020, and 2021 (n = 21,505, age range 46–90 years). We ran logistic regressions on Internet access and Internet use for 4 different gender-typed activities: social contact (female-typed), shopping (gender-neutral), entertainment (male-typed), and banking (male-typed).

Results

Between 2014 and 2021, women drew level with men in Internet access. Gender differences in all 4 forms of Internet use declined considerably between 2014 and 2021. Women overtook men in using the Internet for social contact. In older age groups, men held the lead regarding online banking. During the coronavirus disease 2019 (COVID-19) crisis, women caught up to men in Internet use, especially for entertainment.

Discussion

Overall time trends support the complementary hypothesis. By contrast, the finding that women have been catching up in some male-typed online activities during the COVID-19 pandemic supports the compensatory hypothesis.
The combination of DNA methylation and positive regulation of anthocyanin biosynthesis by MYB and bHLH transcription factors contributes to the petal blotch formation in Xibei tree peony
Authors: Zhu Jin , Wang Yizhou , Wang Qianyu , Li Bing , Wang Xiaohan , Zhou Xian , Zhang Hechen , Xu Wenzhong , Li Shanshan , Wang Liangsheng
Published: May 2023

Xibei tree peony is a distinctive cultivar group that features red–purple blotches in petals. Interestingly, the pigmentations of blotches and non-blotches are largely independent of one another. The underlying molecular mechanism had attracted lots of attention from investigators, but was still uncertain. Our present work demonstrates the factors that are closely related to blotch formation in Paeonia rockii ‘Shu Sheng Peng Mo’. Non-blotch pigmentation is prevented by the silencing of anthocyanin structural genes, among which PrF3H, PrDFR, and PrANS are the three major genes. We characterized two R2R3-MYBs as the key transcription factors that control the early and late anthocyanin biosynthetic pathways. PrMYBa1, which belongs to MYB subgroup 7 (SG7) was found to activate the early biosynthetic gene (EBG) PrF3H by interacting with SG5 member PrMYBa2 to form an ‘MM’ complex. The SG6 member PrMYBa3 interacts with two SG5 (IIIf) bHLHs to synergistically activate the late biosynthetic genes (LBGs) PrDFR and PrANS, which is essential for anthocyanin accumulation in petal blotches. The comparison of methylation levels of the PrANS and PrF3H promoters between blotch and non-blotch indicated a correlation between hypermethylation and gene silencing. The methylation dynamics of PrANS promoter during flower development revealed a potential early demethylating reaction, which may have contributed to the particular expression of PrANS solely in the blotch area. We suggest that the formation of petal blotch may be highly associated with the cooperation of transcriptional activation and DNA methylation of structural gene promoters.
Integrative lipidomics profile uncovers the mechanisms underlying high-level α-linolenic acid accumulation in Paeonia rockii seeds
Authors: Yang Weizong , Xin Ziwei , Xie Lihang , Zhai Yuhui , Zhang Yanlong , Niu Lixin , Zhang Qingyu
Published: May 2023

Tree peony (Paeonia rockii) is an excellent woody oilseed crop, known for its high α-linolenic acid (ALA, ~45%) content, which is of great value for human health. However, the mechanisms underlying this high-level ALA accumulation in tree peony seeds are poorly understood. In this study, we evaluated the dynamic changes in the lipidomic profile of P. rockii seeds during development. A total of 760 lipid molecules were identified in P. rockii seeds; triacylglycerol (TAG) lipid molecules showed the highest abundance and diversity, both increasing during seed development. Particularly, ALA was the predominant fatty acid at the TAG sn-3 position. We further characterized two diacylglycerol acyltransferase (DGAT) genes and three phospholipid:diacylglycerol acyltransferase (PDAT) genes involved in the transfer of fatty acids to the TAG sn-3 position. Gene expression and subcellular localization analyses suggested that PrDGATs and PrPDATs may function as endoplasmic reticulum-localized proteins in seed TAG biosynthesis. In vitro functional complementation analysis showed different substrate specificities, with PrPDAT2 having a specific preference for ALA. Multiple biological assays demonstrated that PrDGAT1, PrDGAT2, PrPDAT1-2, and PrPDAT2 promote oil synthesis. Specifically, PrPDAT2 leads to preferential ALA in the oil. Our findings provide novel functional evidence of the roles of PrDGAT1 and PrPDAT2, which are potential targets for increasing the ALA yield in tree peony and other oilseed crops.
1-Year Comparative Effectiveness of Tofacitinib vs Ustekinumab for Patients With Ulcerative Colitis and Prior Antitumor Necrosis Factor Failure
Authors: Dalal Rahul S , Sharma Puza P , Bains Kanwal , Pruce Jordan C , Allegretti Jessica R
Published: May 2023

Background

Tofacitinib is an oral Janus kinase inhibitor for the treatment of ulcerative colitis (UC). Real-world data comparing the effectiveness of tofacitinib to ustekinumab are limited. We compared 52-week outcomes of tofacitinib vs ustekinumab for UC after antitumor necrosis factor (anti-TNF) failure.

Methods

In this retrospective cohort study, adults initiated tofacitinib or ustekinumab for UC after anti-TNF failure May 1, 2018 to April 1, 2021, at a US academic medical center. The primary outcome was steroid-free clinical remission (SFCR) at 12 and 52 weeks. The secondary outcome was drug survival (ie, time to drug discontinuation due to nonresponse). Adverse events (AEs) were also assessed.

Results

Sixty-nine patients initiated tofacitinib, and 97 patients initiated ustekinumab with median follow-up of 88.0 and 62.0 weeks, respectively. After inverse probability of treatment-weighted logistic and Cox regression, there was no association of tofacitinib vs ustekinumab with SFCR at 12 weeks (odds ratio, 1.65; 95% CI, 0.79-3.41), SFCR at 52 weeks (odds ratio, 1.14; 95% CI, 0.55-2.34), or drug survival (hazard ratio, 1.37; 95% CI, 0.78-2.37). Kaplan-Meier analysis demonstrated no separation in drug survival curves. Regression results were similar after excluding patients with prior tofacitinib or ustekinumab exposure. During available follow-up, 17 AEs were reported for tofacitinib (most commonly shingles, n = 4), and 10 AEs were reported for ustekinumab (most commonly arthralgia and rash, each n = 2). Two patients discontinued treatment due to AEs (1 tofacitinib for elevated liver enzymes, 1 ustekinumab for arthralgia).

Conclusions

In a real-world UC cohort, tofacitinib and ustekinumab demonstrated similar effectiveness at 52 weeks. Adverse events were consistent with the known safety profiles of these agents.
Is it time to abandon the theory of constituent power?
Authors: Verdugo Sergio
Published: May 2023

A group of scholars has shown that the theory of constituent power—which seeks to describe and justify the dismantling of the constitutional order and its replacement with a new constitution—is flawed. The analytical tools the theory deploys fail to explain how constitution-making processes unfold. Also, the theory has been subject to normative challenges that question its democratic nature. However, the theory remains a mainstream idea in many countries, and some academics have attempted to defend its democratic nature. I claim that those attempts have rendered the theory meaningless or failed to address all of its problems. I then raise two objections. First, the constituent power theory cannot be used to justify most—if any—constitution-making processes without an excessive idealization of the founding moment, but we are yet to understand the actual costs of that idealization. Second, redeemers of the theory need to decide whether constitution-making can operate under reasonably favorable electoral and democratic conditions or not. Ideal conditions are improbable when constitutional change is carried out in response to a crisis. In the unlikely case that these conditions can be met, using an idea of constitutional change as radical as the constituent power theory is not warranted from a normative perspective. I call this the dilemma of constituent power redemption.
Between Impunity and Justice? Exploring Stakeholders’ Perceptions of Colombia’s Special Sanctions (Sanciones Propias) for International Crimes
Authors: Mayans-Hermida Beatriz E , Holá Barbora , Bijleveld Catrien
Published: May 2023

ABSTRACT∞

The peace agreement signed by the Colombian government and the FARC has an innovative sanctioning regime which, based on a restorative approach, offers non-custodial sanctions as a less punitive form of punishment for international crimes. However, given their leniency, these ‘special sanctions’ have caused controversy. Based on qualitative interviews, this study explores the perceptions of different stakeholders concerning various issues related to the special sanctions’ nature, goals, processes, (envisioned) outcomes and challenges. Our findings reveal that most participants perceive these sanctions as a tool that can modestly help repair the damage done, reintegrate (certain) offenders into society, and promote coexistence. Only a limited number of respondents saw these sanctions as punishment. For several participants, the special sanctions may be an alternative accountability measure to prison. However, this seems to depend on meeting certain preconditions, victims’ participation, the type of crime and the offender’s rank and affiliation.
The Type VI Secretion System Contributes to the Invasiveness of Liver Abscess Caused by Klebsiella pneumoniae
Authors: Wang Hairui , Guo Yawen , Liu Zhaoyu , Chang Zhihui
Published: May 2023

Background

Klebsiella pneumoniae liver abscess (KPLA) with extrahepatic migratory infections is defined as invasive KPLA (IKPLA). The type VI secretion system (T6SS) is involved in the pathogenesis of KPLA. We hypothesized that T6SS plays a role in IKPLA.

Methods

16S ribosomal RNA gene sequencing was performed on abscess samples. Polymerase chain reaction (PCR) and reverse-transcription PCR (RT-PCR) was used to validate the expression difference of T6SS hallmark genes. In vitro and in vivo experiments were performed to identify the pathogenic feature of T6SS.

Results

PICRUSt2 predicted that the T6SS-related genes were notably enriched in the IKPLA group. PCR detection of T6SS hallmark genes (hcp, vgrG, and icmF) showed that 197 (81.1%) were T6SS-positive strains. The T6SS-positive strain detection rate in the IKPLA group was higher than in the KPLA group (97.1% vs 78.4%; P < .05). RT-PCR showed that the hcp expression level was markedly increased in IKPLA isolates (P < .05). The T6SS-positive isolates showed higher survival against serum and neutrophil killing (all P < .05). The T6SS-positive K pneumoniae–infected mice had a shorter survival time, higher mortality, and an increased interleukin 6 expression in the liver and lungs (all P < .05).

Conclusions

T6SS is an essential virulence factor for K pneumoniae and contributes to IKPLA.
States as Role Models: Why Some Countries’ Policies Matter More than Others
Authors: Greenhill Brian , Willis Charmaine N
Published: May 2023

We often look to the examples set by other countries when thinking about how to deal with problems at home. But what explains why some countries are more likely to serve as role models than others? Are people influenced mainly by the examples set by countries that are considered to be particularly successful in a given policy area? Or are they influenced to a greater extent by countries that are more familiar to them, or that appear more similar to their own? In this paper, we test these hypotheses using two large (n$\ > {\rm{\ }}$2,000) survey-based experiments conducted in the United States. The first focuses on environmental issues, while the second focuses on human rights issues. In both studies, the respondents were presented with a randomly drawn list of countries and asked to indicate how relevant the examples that these countries set should be to policy discussions in the United States. Our results suggest that the US public's perception of the relevance of other countries’ experiences is driven primarily by the extent to which the respondent perceives the other countries as familiar to them, or similar to the United States, rather than by more objective measures of policy success.
Implementation frameworks for end-to-end clinical AI: derivation of the SALIENT framework
Authors: van der Vegt Anton H , Scott Ian A , Dermawan Krishna , Schnetler Rudolf J , Kalke Vikrant R , Lane Paul J
Published: May 2023

Objective

To derive a comprehensive implementation framework for clinical AI models within hospitals informed by existing AI frameworks and integrated with reporting standards for clinical AI research.

Materials and Methods

(1) Derive a provisional implementation framework based on the taxonomy of Stead et al and integrated with current reporting standards for AI research: TRIPOD, DECIDE-AI, CONSORT-AI. (2) Undertake a scoping review of published clinical AI implementation frameworks and identify key themes and stages. (3) Perform a gap analysis and refine the framework by incorporating missing items.

Results

The provisional AI implementation framework, called SALIENT, was mapped to 5 stages common to both the taxonomy and the reporting standards. A scoping review retrieved 20 studies and 247 themes, stages, and subelements were identified. A gap analysis identified 5 new cross-stage themes and 16 new tasks. The final framework comprised 5 stages, 7 elements, and 4 components, including the AI system, data pipeline, human-computer interface, and clinical workflow.

Discussion

This pragmatic framework resolves gaps in existing stage- and theme-based clinical AI implementation guidance by comprehensively addressing the what (components), when (stages), and how (tasks) of AI implementation, as well as the who (organization) and why (policy domains). By integrating research reporting standards into SALIENT, the framework is grounded in rigorous evaluation methodologies. The framework requires validation as being applicable to real-world studies of deployed AI models.

Conclusions

A novel end-to-end framework has been developed for implementing AI within hospital clinical practice that builds on previous AI implementation frameworks and research reporting standards.
Excavation and characterization of key circRNAs for milk fat percentage in Holstein cattle
Authors: Feng Xiaofang , Cai Zhengyun , Gu Yaling , Mu Tong , Yu Baojun , Ma Ruoshuang , Liu Jiaming , Wang Chuanchuan , Zhang Juan
Published: May 2023

Milk fat percentage is one of the significant indicators governing the price and quality of milk and is regulated by a variety of non-coding RNAs. We used RNA sequencing (RNA-seq) techniques and bioinformatics approaches to explore potential candidate circular RNAs (circRNAs) regulating milk fat metabolism. After analysis, compared with low milk fat percentage (LMF) cows, 309 circRNAs were significantly differentially expressed in high milk fat percentage (HMF) cows. Functional enrichment and pathway analysis revealed that the main functions of the parental genes of differentially expressed circRNAs (DE-circRNAs) were related to lipid metabolism. We selected four circRNAs (Novel_circ_0000856, Novel_circ_0011157, novel_circ_0011944, and Novel_circ_0018279) derived from parental genes related to lipid metabolism as key candidate DE-circRNAs. Their head-to-tail splicing was demonstrated by linear RNase R digestion experiments and Sanger sequencing. However, the tissue expression profiles showed that only Novel_circ_0000856, Novel_circ_0011157, and Novel_circ_0011944 were expressed with high abundance in breast tissue. Based on the subcellular localization found that Novel_circ_0000856, Novel_circ_0011157, and Novel_circ_0011944 mainly function as competitive endogenous RNAs (ceRNAs) in the cytoplasm. Therefore, we constructed their ceRNA regulatory networks, and the five hub target genes (CSF1, TET2, VDR, CD34, and MECP2) in ceRNAs were obtained by CytoHubba and MCODE plugins in Cytoscape, as well as tissue expression profiles analysis of target genes. These genes play a key role as important target genes in lipid metabolism, energy metabolism, and cellular autophagy. The Novel_circ_0000856, Novel_circ_0011157, and Novel_circ_0011944 regulate the expression of hub target genes through interaction with miRNAs and constitute key regulatory networks that may be involved in milk fat metabolism. The circRNAs obtained in this study may act as miRNA sponges and thus influence mammary gland development and lipid metabolism in cows, which improves our understanding of the role of circRNAs in cow lactation.
Inhalation Injury: Which Providers Can Assess the Need for Intubation?
Authors: Perkins Louis , Horita Henry , Adams Laura , Marshall William , Lee Jeanne , Doucet Jay , Smith Alan , Santorelli Jarrett E
Published: May 2023

Previous studies have suggested that many burn patients undergo unnecessary intubation due to concern for inhalation injury. We hypothesized that burn surgeons would intubate burn patients at a lower rate than non-burn acute care surgeons (ACSs). We performed a retrospective cohort study of all patients admitted to an American Burn Association-verified burn center who presented emergently following burn injury from June 2015 to December 2021. Patients excluded include polytrauma patients, isolated friction burns, and patients intubated prior to hospital arrival. Our primary outcome was intubation rates between burn and non-burn ACSs. 388 patients met inclusion criteria. 240 (62%) patients were evaluated by a burn provider and 148 (38%) were evaluated by a non-burn provider; the groups were well-matched. In total, 73 (19%) of patients underwent intubation. There was no difference in the rate of emergent intubation, diagnosis of inhalation injury on bronchoscopy, time to extubation, or incidence of extubation within 48 hours between burn and non-burn ACSs. We found no difference between burn and non-burn ACSs in the airway evaluation and management of burn patients. Surgical providers with acute care surgery backgrounds and Advanced Trauma Life Support training are well-equipped for initial airway management in burn patients. Further studies should seek to compare other types of provider groups to identify opportunities for intervention and education in preventing unnecessary intubations.
Product liability and firm owners’ delegation to overconfident managers
Authors: Friehe Tim , Pham Cat Lam
Published: May 2023

This article analyzes the socially optimal liability allocation when strictly liable Cournot firms delegate their safety and output choices to managers whose potential biases are chosen by firm owners and consumers misperceive product risks. Firm owners always hire managers who are overconfident about their product safety’s effectiveness in reducing product-related accident risk. However, the extent of overconfidence depends on consumers’ risk perceptions and the allocation of liability. As a result, the socially optimal liability allocation hinges on whether consumers underestimate or overestimate product risk. When consumers overestimate product risks, firms should be held liable for all losses incurred by consumers. However, when consumers underestimate risk, firms should only be held liable for a part of consumer losses. We also show that, in some circumstances, negligence produces socially more desirable outcomes than strict liability (JEL: K13, L13, L14).
Experimental oral delivery of the systemic acaricide moxidectin to free-ranging white-tailed deer (Artiodactyla: Cervidae) parasitized byAmblyomma americanum(Ixodida: Ixodidae)
Authors: Williams Scott C , Linske Megan A , DeNicola Anthony J , DeNicola Vickie L , Boulanger Jason R
Published: May 2023

Orally delivered, host-targeted, systemic acaricide treatment has potential to be an effective areawide tick abatement strategy. Past efforts using ivermectin for livestock were reported effective at controlling both Amblyomma americanum (L.) and Ixodes scapularis Say on Odocoileus virginianus (Zimmermann). However, the labeled 48-day withdrawal period for human consumption largely prevented utilization of this strategy targeting I. scapularis in autumn, when peak adult host-seeking activity coincides with regulated white-tailed deer hunting seasons. The modern-day compound moxidectin is the active ingredient in the pour-on formulation Cydectin (5 mg moxidectin/ml; Bayer Healthcare LLC), with a labeled 0-day withdrawal period for human consumption of treated cattle. We sought to re-examine the systemic acaricide approach for tick management by determining if we could successfully deliver Cydectin to free-ranging white-tailed deer. Over 2 yr in late spring/early summer, coinciding with adult and nymphal A. americanum activity, we fed Cydectin-coated corn to free-ranging white-tailed deer in coastal Connecticut. Through serum analysis, we documented moxidectin levels at or above those previously reported effective for control of ectoparasites (5–8 ppb for moxidectin and ivermectin) in 24 of 29 white-tailed deer captured (83%) while exposed to treated corn. While we did not document differences in burdens of parasitizing A. americanum based on moxidectin sera levels, we did document fewer engorged specimens on deer with increased sera levels. The systemic use of moxidectin for tick management in critical reproductive hosts has the potential to be effective in an areawide capacity while also permitting human consumption of treated venison.
No Thanks, Dear AI! Understanding the Effects of Disclosure and Deployment of Artificial Intelligence in Public Sector Recruitment
Authors: Keppeler Florian
Published: May 2023

Applications based on artificial intelligence (AI) play an increasing role in the public sector and invoke political discussions. Research gaps exist regarding the disclosure effects—reactions to disclosure of the use of AI applications—and the deployment effect—efficiency gains in data savvy tasks. This study analyzes disclosure effects and explores the deployment of an AI application in a preregistered field experiment (n = 2,000) co-designed with a public organization in the context of employer-driven recruitment. The linear regression results show that disclosing the use of the AI application leads to significantly less interest in an offer among job candidates. The explorative analysis of the deployment of the AI application indicates that the person–job fit determined by the leaders can be predicted by the AI application. Based on the literature on algorithm aversion and digital discretion, this study provides a theoretical and empirical disentanglement of the disclosure effect and the deployment effect to inform future evaluations of AI applications in the public sector. It contributes to the understanding of how AI applications can shape public policy and management decisions, and discusses the potential benefits and downsides of disclosing and deploying AI applications in the public sector and in employer-driven recruitment.
Who Owns the Future of Syrians in Lebanon? Intimate Family Explorations of Refugees’ Own Search for Durable Solutions
Authors: Alpes Maybritt Jill , Feukeu Kwamou Eva , van Houte Marieke , Kseibi Shahed , Shukair Belal
Published: May 2023

For both political and ideological reasons, return is the most favoured future imagined for refugees by policy makers and protection actors. This article analyses how humanitarian migrants in a context of limited durable solutions can be supported to reclaim ownership of their futures, as well as how this can result in deeper insights for social scientists and policy makers. For the case of Syrians, this study deploys futures literacy labs as a participatory and capability-based research methodology that allows participants to become researchers of their own lives. Based on two futures literacy labs with two Syrian families in Lebanon in 2020 and 2021, the article demonstrates that a futures capability-based approach provides humanitarian migrants with the cognitive space and agency needed to go beyond foreclosed decision-making processes. The research methodology allows researchers to become witnesses to intimate reappropriation and learning processes by humanitarian migrants themselves. As a result, we are able to argue that ‘returns’ as a durable solution are essentially about a return to a state of well-being and possibilities, which may or not entail a spatial return.
Maize ZmWRKY28: a target to regulate shade avoidance response under high planting density
Authors: Islam Nishat S
Published: May 2023

This article comments on:

Zhang Z, Chen L, Yu J. 2023. Maize WRKY28 interacts with the DELLA protein D8 to affect skotomorphogenesis and participates in the regulation of shade avoidance and plant architecture. Journal of Experimental Botany74, 3122–3141.
A Potential Resuscitation Route on Battlefield: Immediate Intraperitoneal Fluid Administration Post-burn Shows Satisfactory Fluid Absorption and Anti-shock Effects
Authors: Liu Zhaoxing , Li Dawei , Ma Jinglong , Liu Xinzhu , Zhang Bohan , Qi Zhaolai , Zhang Wen , Yuan Huageng , Niu Yuezeng , Shen Chuanan
Published: May 2023

Introduction

Timely fluid resuscitation remains the key to the early treatment of severe burns. Intraperitoneal (IP) fluid administration is a simple, rapid resuscitation strategy via a puncture in the abdominal wall. This study aimed to evaluate the fluid absorption and anti-shock effects of IP delivery in the early stage after severe burns.

Materials and Methods

A 30% total body surface area full-thickness burn model was established using male C57BL/6 mice. A total of 126 mice were randomly assigned into six groups (n = 21): the sham injury group (SHAM), the burn group without fluid resuscitation (NR), and the four IP resuscitation groups (IP-A/B/C/D, each being intraperitoneally administered with 60, 80, 100, and 120 mL/kg of sodium lactate Ringer’s solution post-injury). Three-hour post-burn, six mice in each group were randomly selected and sacrificed for blood and tissue sampling to detect the IP fluid absorption rate and evaluate organ damage because of low perfusion. The remaining 15 mice in each group were observed for the vital signs within 48-h post-injury, and their survival rate was calculated.

Results

The 48-h survival rate increased in the IP-A (40.0%), IP-B (66.7%), IP-C (60.0%), and IP-D (13.3%) groups, compared with the NR group (0%). The mean arterial pressure, body temperature, and heart rate of mice were significantly stabilized in the IP groups. For the first 3-h post-injury, the absorption rates of groups IP-A (74.3% ± 9.5%) and IP-B (73.3% ± 6.9%) were significantly higher than those of groups IP-C (59.7% ± 7.1%) and IP-D (48.7% ± 5.7%). The levels of arterial blood pH, partial pressure of oxygen, partial pressure of carbon dioxide, lactate, and hematocrit were better maintained in the IP groups. Intraperitoneal resuscitation remarkably reduced the injury scores in burn-induced histopathology of the liver, kidneys, lungs, and intestines, accompanied by decreased alanine transaminase, creatinine, interleukin-1, and tumor necrosis factor-α in plasma, and augmented superoxide dismutase 2 and inhibited malondialdehyde in tissues. Group IP-B has the best performance for these indices.

Conclusions

Intraperitoneal administration of isotonic saline post-burn can be adequately and rapidly absorbed, thereby boosting circulation and perfusion, precluding shock, alleviating organ damage caused by ischemia and hypoxia, and significantly increasing the survival rate. This technique, with a potential to be a supplement to existing resuscitation methods on the battlefield, is worth further investigation.
National Guard Deployment in Support of COVID-19: Psychological and Behavioral Health
Authors: Mash Holly B Herberman , Fullerton Carol S , Adler Amy B , Morganstein Joshua C , Biggs Quinn M , Ursano Robert J
Published: May 2023

Introduction

The National Guard (NG) served as a critical component of the USA’s response to the Coronavirus Disease 2019 (COVID-19) pandemic, while concurrently managing their personal responses to the pandemic. Determining whether the activation of NG service members in response to the COVID-19 pandemic was associated with a greater psychological strain can identify NG’s needs for mental health support.

Materials and Methods

We surveyed 3993 NG unit (NGU) service members (75% Army NG, 79% enlisted, 52% 30-49 years old, and 81% males) during the COVID-19 pandemic, with surveys administered between August and November 2020. Almost half (46%) of NGU service members reported being activated in response to COVID-19 (mean activation length = 18.6 weeks). Activated service members completed the survey approximately 2 to 3 months post-activation. Surveys assessed demographics, service-related characteristics, unit cohesion and positive leadership skills (leadership), and COVID-19 activation, and outcomes including probable post-traumatic stress disorder (PTSD), clinically significant anxiety and depression, and anger. Descriptive and logistic regression analyses were conducted. The study was approved by the Institutional Review Board of the Uniformed Services University of the Health Sciences in Bethesda, MD.

Results

In all, 9.7% met the criteria for probable PTSD, 7.6% reported clinically significant anxiety and depression, and 13.2% reported feeling angry/anger outbursts. Multivariate logistic regression analyses, adjusting for demographic and service-related characteristics, indicated that COVID-19 activation was not associated with a greater risk of PTSD, anxiety and depression, or anger. Regardless of activation status, NGU service members with low levels of unit cohesion and leadership were more likely to report PTSD and anger, and low levels of unit cohesion were associated with clinically significant anxiety and depression.

Conclusions

COVID-19 activation did not increase the risk of mental health difficulties among NGU service members. However, low levels of unit cohesion were associated with the risk of PTSD, anxiety and depression, and anger, and low levels of leadership were associated with the risk of PTSD and anger. The results suggest a resilient psychological response to COVID-19 activation and the potential for strengthening all NG service members through enhancing unit cohesion and leadership support. Future research on specific activation exposures, including the type of work tasks in which service members are engaged, particularly those associated with high-stress work conditions, is needed to help better understand their activation experience and how it may influence post-activation responses.
Myotonometry is Capable of Reliably Obtaining Trunk and Thigh Muscle Stiffness Measures in Military Cadets During Standing and Squatting Postures
Authors: McGowen Jared M , Hoppes Carrie W , Forsse Jeff S , Albin Stephanie R , Abt John , Koppenhaver Shane L
Published: May 2023

Introduction

Low back and lower extremity injuries are responsible for the highest percentage of musculoskeletal injuries in U.S. Army soldiers. Execution of common soldier tasks as well as army combat fitness test events such as the three-repetition maximum deadlift depends on healthy functioning trunk and lower extremity musculature to minimize the risk of injury. To assist with appropriate return to duty decisions following an injury, reliable and valid tests and measures must be applied by military health care providers. Myotonometry is a noninvasive method to assess muscle stiffness, which has demonstrated significant associations with physical performance and musculoskeletal injury. The aim of this study is to determine the test–retest reliability of myotonometry in lumbar spine and thigh musculature across postures (standing and squatting) that are relevant to common soldier tasks and the maximum deadlift.

Materials and Methods

Repeat muscle stiffness measures were collected in 30 Baylor University Army Cadets with 1 week between each measurement. Measures were collected in the vastus lateralis (VL), biceps femoris (BF), lumbar multifidus (LM), and longissimus thoracis (LT) muscles with participants in standing and squatting positions. Intraclass correlation coefficients (ICCs3,2) were estimated, and their 95% CIs were calculated based on a mean rating, mixed-effects model.

Results

The test–retest reliability (ICC3,2) of the stiffness measures was good to excellent in all muscles across the standing position (ICCs: VL = 0.94 [0.87–0.97], BF = 0.97 [0.93–0.98], LM = 0.96 [0.91–0.98], LT = 0.81 [0.59–0.91]) and was excellent in all muscles across the squatting position (ICCs: VL = 0.95 [0.89–0.98], BF = 0.94 [0.87–0.97], LM = 0.96 [0.92–0.98], LT = 0.93 [0.86–0.97]).

Conclusion

Myotonometry can reliably acquire stiffness measures in trunk and lower extremity muscles of healthy individuals in standing and squatting postures. These results may expand the research and clinical applications of myotonometry to identify muscular deficits and track intervention effectiveness. Myotonometry should be used in future studies to investigate muscle stiffness in these body positions in populations with musculoskeletal injuries and in research investigating the performance and rehabilitative intervention effectiveness.
Modelling supernova nebular lines in 3D with extrass
Authors: van Baal Bart F A , Jerkstrand Anders , Wongwathanarat Annop , Janka Hans-Thomas
Published: May 2023

We present EXplosive TRAnsient Spectral Simulator (extrass), a newly developed code aimed at generating 3D spectra for supernovae in the nebular phase by using modern multidimensional explosion models as input. It is well established that supernovae are asymmetric by nature, and that the morphology is encoded in the line profiles during the nebular phase, months after the explosion. In this work, we use extrass to study one such simulation of a $3.3\, \mathrm{ M}_\odot$ He-core explosion ($M_\text{ejecta}=1.3\, M_\odot$, $E_\text{kin}=1.05\times 10^{51}\,$erg) modelled with the Prometheus-HotB code and evolved to the homologous phase. Our code calculates the energy deposition from the radioactive decay of 56Ni → 56Co → 56Fe and uses this to determine the Non-Local-Thermodynamic-Equilibrium temperature, excitation, and ionization structure across the nebula. From the physical condition solutions we generate the emissivities to construct spectra depending on viewing angles. Our results show large variations in the line profiles with viewing angles, as diagnosed by the first three moments of the line profiles; shifts, widths, and skewness. We compare line profiles from different elements, and study the morphology of line-of-sight slices that determine the flux at each part of a line profile. We find that excitation conditions can sometimes make the momentum vector of the ejecta emitting in the excited states significantly different from that of the bulk of the ejecta of the respective element, thus giving blueshifted lines for bulk receding material, and vice versa. We compare the 3.3 M⊙ He-core model to observations of the Type Ib supernova SN 2007Y.
A mutually embedded perception model for solar corona
Authors: Zhao Jingmin , Feng Xueshang , Xiang Changqing , Jiang Chaowei
Published: May 2023

This paper proposes a new mutually embedded perception model (MEPM) based on the 3D magnetohydrodynamic (MHD) equations of the solar wind plasma to reconstruct the structure of the solar corona. The goal is to embed the physics-based information and gradient into solar wind parameters data through the neural network and leverage the adaptive procedures to improve solution accuracy. The loss term proportional to the divergence is directly introduced to force a divergence-free solution. The established MEPM displays almost the same results as the exact solution for an artificial 3D analytic problem and the Parker solar wind for 1D steady Parker flow with the corresponding boundary conditions. The MEPM can well capture the solar coronal leading structures, recover the results of the traditional numerical schemes, and be consistent with the observations with CR 2068 as an example. When supplementary data (from the results of the MHD simulation or empirical models) are used, the modeled results improve. This implies that in situ satellite observations as supplementary data can be incorporated into the model in the same way.
CLEAN imaging systematics of M87 radio jet
Authors: Pashchenko I N , Kravchenko E V , Nokhrina E E , Nikonov A S
Published: May 2023

The spectral index images of the jet in the nearby radio galaxy M87 have previously been shown with Very Long Baseline Interferometric arrays at 2–43 GHz. They exhibit flattening of the spectra at a location of inner (central) spine and towards outer ridges. This could imply optical depth effects, lower energy cut-off, or stratification of the emitting particles energy distribution. In this paper, we employ simulations of multifrequency VLBI observations of M87 radio jet with various model brightness distributions. CLEAN deconvolution errors produce significant features in the observed images. For intensity images, they result in the appearance of the inner ridge line in the intrinsically edge brightened jet models. For spectral index images, they flatten the spectra in a series of stripes along the jet. Another bias encountered in our simulations is steepening of the spectra in a low surface brightness jet regions. These types of the imaging artefacts do not depend on the model considered. We propose a method for the compensation of the systematics using only the observed data.