bluuebunny/crossref_metadata_2025 huggingface dataset:
Influences of the Periodicity in Molecular Architecture on the Phase Diagrams and Microphase Transitions of the Janus Double-Brush Copolymer with a Loose Graft
Authors: Sun Dachuan , Song Yang
Published: July 2022

The backbone of the Janus double-brush copolymer may break during long-term service, but whether this breakage affects the self-assembled phase state and microphase transitions of the material is still unknown. For the Janus double-brush copolymers with a periodicity in molecular architecture ranging from 1 to 10, the influences of the architectural periodicity on their phase diagrams and order–disorder transitions (ODT) were investigated by the self-consistent mean field theory (SCFT). In total, nine microphases with long-range order were found. By comparing the phase diagrams between copolymers of different periodicity, a decrease in periodicity or breakage along the copolymer backbone had nearly no influence on the phase diagrams unless the periodicity was too short to be smaller than 3. For copolymers with neutral backbones, a decrease in periodicity or breakage along the copolymer backbone reduced the critical segregation strengths of the whole copolymer at ODT. The equations for the critical segregation strengths at ODT, the architectural periodicity, and the volume fraction of the backbone were established for the Janus double-brush copolymers. The theoretical calculations were consistent with the previous theoretical, experimental, and simulation results.
Fabrication of Tissue-Engineered Cartilage Using Decellularized Scaffolds and Chondrocytes
Authors: Lu Liang , Shang Xifu , Liu Bin , Chen Weijian , Zhang Yu , Liu Shuyun , Sui Xiang , Wang Aiyuan , Guo Quanyi
Published: July 2022

In this paper, we aim to explore the application value of tissue engineering for the construction of artificial cartilage in vitro. Chondrocytes from healthy porcine articular cartilage tissue were seeded on articular cartilage extracellular matrix (ACECM) scaffolds and cultivated. Type II collagen immunofluorescent staining was used to assess secretion from the extracellular matrix. Chondrocytes, which were mainly polygonal and cobblestone-shaped, were inoculated on ACECM-oriented scaffolding for 7 days, and the neo-tissue showed translucent shape and toughness. Using inverted and fluorescence microscopy, we found that chondrocytes on the scaffolds performed well in terms of adhesion and growth, and they secreted collagen type II. Moreover, the porcine ACECM scaffolds had good biocompatibility. The inflammatory cell detection, cellular immune response assay and humoral immune response assay showed porcine ACECM scaffolds were used for xenotransplantation without significant immune inflammatory response. All these findings reveal that ACECM-oriented scaffold is an ideal natural biomaterial for cartilage tissue engineering.
Application of Atomic Force Microscopy as Advanced Asphalt Testing Technology: A Comprehensive Review
Authors: Ouyang Qijian , Xie Zhiwei , Liu Jinhai , Gong Minghui , Yu Huayang
Published: July 2022

In the past three decades, researchers have engaged in the relationship between the composition, macro performance, and microstructure of asphalt. There are many research results in the use of atomic force microscopy (AFM) to study the microstructure and related mechanisms of asphalt. Based on previous studies, the performance of asphalt from its microstructure has been observed and analyzed, and different evaluation indices and modification methods have been proposed, providing guidance toward improving the performance of asphalt materials and benefiting potential applications. This review focuses on the typical application and analysis of AFM in the study of the aging regeneration and modification properties of asphalt. Additionally, this review introduces the history of the rheological and chemical testing of asphalt materials and the history of using AFM to investigate asphalt. Furthermore, this review introduces the basic principles of various modes of application of AFM in the microstructure of asphalt, providing a research direction for the further popularization and application of AFM in asphalt or other materials in the future. This review aims to provide a reference and direction for researchers to further popularize the application of AFM in asphalt and standardize the testing methods of AFM. This paper is also helpful in further exploring the relationship between the microstructure and macro performance of asphalt.
The Use of Carbohydrate Biopolymers in Plant Protection against Pathogenic Fungi
Authors: Korbecka-Glinka Grażyna , Piekarska Klaudia , Wiśniewska-Wrona Maria
Published: July 2022

Fungal pathogens cause significant yield losses of many important crops worldwide. They are commonly controlled with fungicides which may have negative impact on human health and the environment. A more sustainable plant protection can be based on carbohydrate biopolymers because they are biodegradable and may act as antifungal compounds, effective elicitors or carriers of active ingredients. We reviewed recent applications of three common polysaccharides (chitosan, alginate and cellulose) to crop protection against pathogenic fungi. We distinguished treatments dedicated for seed sowing material, field applications and coating of harvested fruits and vegetables. All reviewed biopolymers were used in the three types of treatments, therefore they proved to be versatile resources for development of plant protection products. Antifungal activity of the obtained polymer formulations and coatings is often enhanced by addition of biocontrol microorganisms, preservatives, plant extracts and essential oils. Carbohydrate polymers can also be used for controlled-release of pesticides. Rapid development of nanotechnology resulted in creating new promising methods of crop protection using nanoparticles, nano-/micro-carriers and electrospun nanofibers. To summarize this review we outline advantages and disadvantages of using carbohydrate biopolymers in plant protection.
Prediction of the Glass Transition Temperature in Polyethylene Terephthalate/Polyethylene Vanillate (PET/PEV) Blends: A Molecular Dynamics Study
Authors: Sangkhawasi Mattanun , Remsungnen Tawun , Vangnai Alisa S. , Maitarad Phornphimon , Rungrotmongkol Thanyada
Published: July 2022

Polyethylene terephthalate (PET) is one of the most common polymers used in industries. However, its accumulation in the environment is a health risk to humans and animals. Polyethylene vanillate (PEV) is a bio-based material with topological, mechanical, and thermal properties similar to PET, allowing it to be used as a PET replacement or blending material. This study aimed to investigate some structural and dynamical properties as well as the estimated glass transition temperature (Tg) of PET/PEV blended polymers by molecular dynamics (MD) simulations with an all-atom force field model. Four blended systems of PET/PEV with different composition ratios (4/1, 3/2, 2/3, and 1/4) were investigated and compared to the parent polymers, PET and PEV. The results show that the polymers with all blended ratios have Tg values around 344–347 K, which are not significantly different from each other and are close to the Tg of PET at 345 K. Among all the ratios, the 3/2 blended polymer showed the highest number of contacting atoms and possible hydrogen bonds between the two chain types. Moreover, the radial distribution results suggested the proper interactions in this system, which indicates that this is the most suitable ratio model for further experimental studies of the PET/PEV polymer blend.
Use of Typical Wastes as Biochars in Removing Diethyl Phthalate (Det) from Water
Authors: Chai Zichun , Bi Xianshuang , Jia Hongbai
Published: July 2022

Diethyl phthalate (DEP), one of the six typical PAEs priority pollutants declared by the US EPA, has attracted tremendous attention due to its widespread pollution and was selected as the adsorbate in this study. Properties of biochar samples obtained from three different feedstocks, i.e., sawdust (SDBC), rice straw (RSBC), and giant reed (GRBC), pyrolyzed at 400 °C as well as their ability to adsorb DEP from an aqueous solution were investigated. The results showed that the adsorption kinetics were well fitted with the pseudo-second-order model (R2 > 0.99) and the intraparticle diffusion model (R2 > 0.98). The maximal adsorption capacity of the DEP by the prepared biochar was in an order of GRBC (46.04 mg g−1) > RSBC (31.54 mg g−1) > and SDBC (18.39 mg g−1). The higher adsorption capacity of DEP by GRBC is mainly attributed to the higher surface area. The reduction in adsorption capacity of the biochar against DEP with an increase in the solution pH (from 2.5 to 10.0) was possibly due to promoting the electrostatic repulsion between the DEP and the surface of the biochar. However, the increasing sodium ionic strength promoted the adsorption of the biochar, which could be interpreted by the reduced solubility of the DEP due to enhancing “salting out” effects as increasing sodium concentration. In addition, it was favorable for the adsorption of DEP onto the biochars at a lower temperature (15 °C) and the calculated ∆G0 was less than zero, indicating that the adsorption was a spontaneous and exothermic process. These experiments designate that these derived biochars can be used as an inexpensive adsorbent for the purification of PAEs contaminated water.
The Impact of Cultural Diversity on End-of-Life Care
Authors: Yih Caroline
Published: July 2022

Despite the universality of death for humanity, end-of-life care needs and expectations are highly unique and influenced by the individual’s cultural conditioning, values, and beliefs. In the pursuit of quality end-of-life care provision within the increasingly complex and diverse contemporary medical context, it is vital for cultural idiosyncrasies to be taken into consideration in order to attend to the individual patient’s needs and end-of-life goals. Palliative chaplains, as the spiritual care specialists within the multidisciplinary healthcare team, play a crucial role in the support and facilitation of the holistic vision of end-of-life care delivery. However, the capacity of the chaplains to become culturally competent practitioners are often insufficiently addressed in their professional educational pathways, creating additional challenges for them in their practice. Using Hong Kong as a case study, this article examines the impact of cultural diversity on the effectiveness of the chaplains’ delivery of end-of-life spiritual care. Specifically, special attention will be focused on two identified challenges resulting from the lack of integration of local cultural understandings within the religion-cultural practice framework of chaplaincy formation: the cultural taboo of death, and the cultural idiosyncrasies in end-of-life communication. This article hopes to raise awareness of cultural incongruencies within the current chaplaincy professional formation and development, and to initiate further attention and efforts to support chaplains in becoming culturally competent practitioners in the pluralistic healthcare landscape.
Jesus’ Spirituality of [Af]filiation in the Fourth Gospel
Authors: Lee Dorothy A.
Published: July 2022

The spirituality of Jesus, embedded within the literary contours of the Johannine narrative, is primarily grounded in a relationship of affiliation and friendship. It is a spirituality of abiding whose origins and goal lie in the unity of heart and mind that the Johannine Jesus as Son shares with the Father. This core relationship connotes not only the love that binds Jesus to God but is also the basis of the motif of sending and the divine authority over life and death which Jesus possesses in this Gospel. Jesus’ spirituality is grounded in the abiding presence of the Spirit-Paraclete whom he bequeaths to the disciples. In handing over the Spirit to the gathered community through his death and resurrection, Jesus donates his own spirituality, ultimately drawing all creation into the divine circle of love. This spirituality is the result of the Spirit’s presence, restoring human beings to their original, created identity as children and friends of God and empowering them for mission. While the dominant imagery is masculine there are also feminine images, particularly that of divine Wisdom, which provide a counterbalance and create an inclusive sense of appropriation and welcome.
Improved ENSO and PDO Prediction Skill Resulting from Finer Parameterization Schemes in a CGCM
Authors: Yang Yuxing , Hu Xiaokai , Liao Guanghong , Cao Qian , Chen Sijie , Gao Hui , Wei Xiaowei
Published: July 2022

Coupled general circulation models (CGCMs), as tools of predicting climate variability, are constantly being improved due to their immense value in a host of theoretical and practical, real-world problems. Consequently, four new parameterization schemes are introduced in the First Institute of Oceanography Earth System Model (FIO-ESM), and a new climate prediction System (CPS) is built up based on modified and original FIO-ESM. Here, turbulence from the sea surface to deep ocean were fully described, and seasonal forecasts of El Niño-Southern Oscillation (ENSO) and year-to-year prediction of Pacific Decadal Oscillation (PDO) were made with both the modified and original FIO-ESM-CPS. The results illustrate that the anomaly correlation coefficient (ACC) of the Niño 3.4 index significantly increased, and the root mean square error (RMSE) significantly decreased, respectively, in the modified FIO-ESM-CPS as compared to the original. The RMSE is improved by over 20% at 4- and 5-month lead times. Over longer leads, and in the modified FIO-ESM-CPS, forecast ENSO amplitudes are far closer to observations than the original CGCM, which significantly overestimates amplitudes. PDO prediction skill is also improved in the modified FIO-ESM-CPS with ACC improving by 36% at the 4-year lead time and RMSE decreasing by 21% at the 3-year lead time.
A Novel Approach for the Global Detection and Nowcasting of Deep Convection and Thunderstorms
Authors: Müller Richard , Barleben Axel , Haussler Stéphane , Jerg Matthias
Published: July 2022

Thunderstorms are among the most common and most dangerous meteorological hazards in the world. They cause lightning and can lead to strong wind gusts, squall lines, hail and heavy precipitation combined with flooding, and therefore pose a threat to health and life, can cause enormous property damage and also endanger flight safety. Monitoring and forecast of thunderstorms are, therefore, important topics. In this work, a novel method for the detection and forecast of thunderstorms and strong convection is presented. The detection is based on the global GLD360 lightning data in combination with satellite information from the satellite series Meteosat, HIMAWARI and GOES, covering the complete geostationary ring. Three severity levels are defined depending on the occurrence of lightning and the brightness temperature difference of the water vapour channels and the infrared window channel (∼10.8 μm). The detection of thunderstorms and strong convection is the basis for the nowcasting up to 2 h, which is performed with the optical flow method TV-L1. This method provides the needed atmospheric motion vectors for the extrapolation of the thunderstorm movement. Both, the validation results as well as the feedback of the customers show the great value of the new NowCastSat-Aviation (NCS-A) method. For example, the Critical Success Index (CSI) is, with 0.64, still quite high for the 60 min forecast of severe thunderstorms. The method is operated 24/7 by the German Weather Service (DWD), and is used to provide thunderstorm information to aviation customers and the central weather forecast unit of DWD.
Adding UVA and Far-Red Light to White LED Affects Growth, Morphology, and Phytochemicals of Indoor-Grown Microgreens
Authors: Hooks Triston , Sun Ling , Kong Yun , Masabni Joseph , Niu Genhua
Published: July 2022

White light emitting diodes (LED) have commonly been used as a sole light source for the indoor production of microgreens. However, the response of microgreens to the inclusion of ultraviolet A (UVA) and/or far-red (FR) light to white LED light remains unknown. To investigate the effects of adding UVA and FR light to white LEDs on plant biomass, height, and the concentrations of phytochemicals, four species of microgreens including basil, cabbage, kale, and kohlrabi were grown under six light treatments. The first three treatments were white LED (control) and two UVA treatments (adding UVA to white LED for the whole growth period or for the last 5 days). Another three treatments consisted of adding FR to the first three treatments. The total photon flux density (TPFD) for all six light treatments was the same. The percentages of UVA and FR photons in the TPFD were 23% and 32%, respectively. Compared to white LEDs, adding UVA throughout the growth period did not affect plant height in all the species except for basil, where 9% reduction was observed regardless of the FR light. On the contrary, the addition of FR light increased plant heights by 9–18% for basil, cabbage, and kohlrabi, regardless of the UVA treatment, compared to white LED. Furthermore, regardless of UVA, adding FR to white LEDs reduced the plant biomass, total phenolic contents, and antioxidant concentrations for at least one species. There was no interaction between FR and UVA on all the above growth and quality traits for all the species. In summary, microgreens were more sensitive to the addition of FR light compared to UVA; however, the addition of FR to white LEDs may reduce yields and phytochemicals in some species.
A Multi-Objective Optimization Model for the Intercity Railway Train Operation Plan: The Case of Beijing-Xiong’an ICR
Authors: Fan Zilong , Liu Di , Rong Wenyu , Li Chengrui
Published: July 2022

For intercity railway transportation enterprises, a reasonable intercity train operation plan is not only the foundation of the intercity railway operation organization, but also the key to the sustainable development of the intercity railway (ICR). In this paper, taking into account the economic benefits of railway transportation enterprises and the social benefits of passenger travel, an optimization model is established with the intercity railway train operation plan as the research object. The model aims to minimize the operating cost of railway transportation enterprises and minimize the travel time of passengers, and considers constraints such as passenger seat utilization, passenger flow, train frequency, and stops. It is a multi-objective optimization model that accumulates two objectives by introducing the passenger time value coefficient. According to the characteristics of the model, a genetic algorithm is designed to solve the model. Taking the Beijing-Xiong’an Intercity Railway (BXICR) as an example, the “smart business card” of China’s high-speed railway, two scenarios of passenger time value are designed, and the optimized train operation plan is obtained according to the existing OD passenger flow data, which verifies the effectiveness of the model and algorithm. The results show that compared with the original train operation plan, the number of stops per train of the optimized train operation plan under the two passenger time value scenarios decreased by 8.8% and 14.9%, the operating cost of the enterprise decreased by 7.7% and 1.6%, the travel time of passengers decreased by 0.7% and 1.5%, respectively. Under the condition of meeting the demand of passenger flow, the optimized train operation plan can effectively reduce the operating cost of enterprises and save the travel time of passengers, which is conducive to the sustainable development of intercity railways.
Does Gender Matter? The Relationship Comparison of Strategic Leadership on Organizational Ambidextrous Behavior between Male and Female CEOs
Authors: Zhang Gang , Jia Ziang , Yan Shimei
Published: July 2022

This paper aims to examine the relationship difference of strategic leadership to organizational ambidextrous behavior between male and female CEOs, taking into account the balancing effect and combined effect of explorative and exploitative behaviors. A quantitative analysis is conducted from demographic data and relevant organizational ambidextrous data of male and female CEOs of listed companies from 2016 to 2020. In total, 226 valid male-female CEOs pairs are used to test whether there is a significant difference on gender between strategic leadership and organizational ambidextrous behavior. The findings revealed that female strategic leaders with an ambidextrous advantage do not behave inferior to males. This study clarifies the intrinsic relationship between female strategic leadership and organizational ambidexterity, affirming the ambidextrous synergistic effect of female executives in corporate strategic decision-making. The presentation of women in top management may contribute to a better organizational performance through balancing and combining ambidextrous activities, and this study calls on upholding the cultural values of gender diversity and inclusiveness, and to focus on merits of female leaders.
The Scope for Using Proximal Soil Sensing by the Farmers of India
Authors: Behera Sanjib Kumar , Adamchuk Viacheslav I. , Shukla Arvind Kumar , Pandey Punyavrat Suvimalendu , Kumar Pardeep , Shukla Vimal , Thiyagarajan Chitdeshwari , Rai Hitendra Kumar , Hadole Sandeep , Sachan Anil Kumar , Singh Pooja , Trivedi Vivek , Mishra Ashutosh , Butail Nagender Pal , Kumar Praveen , Prajapati Rahul , Tiwari Kshitij , Suri Deepika , Sharma Munish
Published: July 2022

Knowledge about spatial distribution patterns of soil attributes is very much needed for site-specific soil nutrient management (SSSNM) under precision agriculture. High spatial heterogeneity exists in the agricultural soils of India due to various reasons. The present practice of assessing the spatial variability of the vast cultivated landscape of India by using traditional soil sampling and analysis is costly and time consuming. Hence, proximal soil sensing (PSS) is an attractive option to assess the plot-scale spatial variability pattern (SVP) of soil attributes for SSSNM. A PSS system, either in a fixed position or mounted on a vehicle (on-the-go), can be used to obtain measurements by having direct contact with soil. PSS measurements provide low-cost and high-density data pertaining to the SVPs of soil attributes. These data can be used to generate digital elevation and soil attribute variability maps at the field scale in a crop production environment. Based on the generated variability maps, locally available and economically feasible agricultural inputs can be applied using variable rate application strategies for sustainable cropping and enhanced farm profit. This overview presents the potential of adopting PSS in India and other developing countries. The scope, challenges, and probable solutions are also proposed. There is ample scope for adoption of PSS in India in view of diverse soil types, climatic conditions, cropping patterns, crop management practices, and ultimately, the ever-increasing demand for higher agricultural production. However, the successful adoption of the PSS technique in India will be dependent on the proper design and adoption of strategies which require adequate planning and analysis. There are several studies that have highlighted the usefulness of soil sensing technologies in Indian soils. There are also certain challenges and limitations associated with PSS in India, which could be addressed. The available proximal soil sensing technologies will be of great help in improving the understanding of soil heterogeneity for adopting SSSNM in order to optimize crop production in India and other developing countries.
Perceived Value in Sustainable Coastal and Marine Destinations: A Study of Jacó in Costa Rica
Authors: Carvache-Franco Mauricio , Víquez-Paniagua Ana Gabriela , Carvache-Franco Wilmer , Pérez-Orozco Allan , Carvache-Franco Orly
Published: July 2022

Perceived value influences the future behavior of tourists in sustainable coastal destinations, and thus is crucial for the elaboration of development plans. This study aims to: (i) identify the dimensions of the perceived value of demand in a coastal and marine destination; (ii) determine the dimensions of the perceived value that predict the satisfaction of the demand of a coastal and marine destination; and (iii) establish the dimensions of perceived value that predict loyalty variables such as return, recommendation, and saying positive things about a coastal and marine destination. This research was carried out in Jacó, a sustainable tourist destination on the Pacific Ocean coast located in the Central American country of Costa Rica. The sample collection was carried out on-site in June 2021 during the COVID-19 pandemic. This quantitative study used a sample of 304 valid questionnaires. Factor analysis and the multiple regression method were performed for data analysis. The results show three dimensions in the perceived value, namely, the emotional and functional value, the social value, and the economic value. The emotional and functional dimension is the most important predictor of satisfaction and loyalty in variables such as return, recommendation, and saying positive things about a coastal and marine destination. The results can serve as a management guide for managers of coastal and marine destinations and as information for service providers to develop products according to demand.
Auto-Rickshaw Repair, Servicing and Maintenance for Youth-in-Entrepreneurship in Kumasi
Authors: Owusu-Ansah Prince , Obiri-Yeboah Abena Agyeiwaa , Abdul-Aziz A. R. , Woangbah Saviour Kwame , Nyantakyi Emmanuel Kwesi , Asamoah Jack Nti
Published: July 2022

Knowledge and skill acquisition is a driver to the socioeconomic growth of a country, yet economic challenges, rapid urbanization, and migration have contributed to youth unemployment in Ghana. The introduction of auto-rickshaws in 2015 has increased access coverage to transportation, augmented existing public transportation, and created avenues for both operators’ and mechanics’ employment through co-ordinated skill development actions to empower youth-in-entrepreneurship. However, these have not been fully utilized and developed by the Government. The study seeks to identify basic needs of auto-rickshaw mechanics in establishing a sustainable repair and maintenance enterprise, identify challenges confronting auto-rickshaw mechanics, and analyze activities of the youth who repair, maintain, and service auto-rickshaws within the formal and informal sectors for sustainable enterprises. The study is underpinned by both resource-based and human capital theories: education and apprenticeship bothers on human capital theory, while entrepreneurial experience, shop space, business duration, and so on projects the resource-based theory. Questionnaires were administered to 237 auto-rickshaw mechanics and interviews were conducted for some selected auto-rickshaw mechanics to investigate challenges in the business in the Kumasi Metropolitan Area through a clustered and systematic sampling technique. Data analyzed using SPSS v.26 indicated a very youthful auto-rickshaw mechanic population with low educational level that operates without licensed garages. About 68.8% of the mechanics possessed some form of apprenticeship training, though they are not specific to auto-rickshaw repair and maintenance. Further, 71.7% auto-rickshaw mechanics do not keep records of their activities and there is a general lack of entrepreneurial skills among them. Lack of financial support from financial institutions was found to be a major challenge. The binary logistic regression model retained and confirmed six out of the seven predictors, including sex, age, education, marital status, duration of repairs and/or maintenance, shop space, tools and equipment, and mechanical training, as significant contributing factors to entrepreneurship training offered to enterprises within the Kumasi metropolis. The study recommends the urgent need to upgrade the technical and entrepreneurial skills of auto-rickshaw mechanics through the establishment of targeted and result-oriented training centers for better efficiency. The authors further recommend financial institutions to consider giving financial support to auto-rickshaw mechanics to help sustain their businesses.
A Sustainability Assessment Framework for On-Site and Off-Site Construction Logistics
Authors: Brusselaers Nicolas , Fufa Selamawit Mamo , Mommens Koen
Published: July 2022

Urban areas pay increasing attention to new construction and infrastructure works, mainly due to the rapid global rise in urbanisation. In the long run, these works have a positive correlation with the economic and social attractiveness of cities. Construction strongly relies on logistics activities, which cannot be neglected in the environmental equation. An important aspect in tackling the negative effects of construction logistics (CL) lies in understanding the source and mitigation potential of the impacts incurred. However, currently, limited robust impact assessments are available for this sector. Given the lack of these rigorous assessments, it is difficult to evaluate the environmental criteria concerned, especially when comparing innovative CL solutions. In this paper, we present a holistic sustainability assessment framework designed for CL activities based on life cycle approaches, which covers four main iterative steps: (1) goal and scope definition, (2) data identification and availability, (3) scenario and setup evaluation and (4) environmental impact assessment. To measure both the off-site and on-site CL impact, two distinct and complementary methodologies are used: External Cost Calculations and Life Cycle Assessment. The framework was implemented on a pilot case in the Brussels-Capital Region (Belgium). It provides a holistic view of CL impacts for policy evaluations and implementations on the project, portfolio or city level. The results show that off-site zero-emission construction vehicles are the way forward if cities want to achieve environmental goals by 2035. However, market readiness for high-capacity vehicles must be considered. Otherwise, the positive effects on air pollution, climate change and noise are offset by a saturation of the road transport network and its associated congestion and infrastructure damage costs.
Application of the Response Surface Methodology (RSM) in the Optimization of Acenaphthene (ACN) Removal from Wastewater by Activated Carbon
Authors: Moria Kawthar Mostafa , Khurshid Hifsa , Mustafa Muhammad Raza Ul , Alhothali Areej , Bamasag Omaimah Omar
Published: July 2022

The presence of polycyclic aromatic hydrocarbons (PAHs) in wastewater has raised concerns about human health due to their potential carcinogenic and mutagenic properties. The widespread use of products containing acenaphthene (ACN, one of the 16 priority PAHs) in many industries and large-scale ACN release into the wastewater has resulted in dangerous concentrations of ACN in the environment. As a result, before discharge, it is required to eliminate or reduce its concentration to an acceptable level. Adsorption is an effective method of removing PAHs from wastewater. In this study, the ACN adsorption reaction in sample wastewater was evaluated using activated carbon produced by oil palm leaves. HPLC was used as an analytical method for quantifying ACN in wastewater samples. The initial concentration of ACN in water samples was 9.58 ± 0.5 mg/L. The experiments were conducted using the CCD combined with the RSM and using three independent variables, i.e., pH, activated carbon dosage (g/L), and contact time (min), and one dependent variable, i.e., ACN removal efficiency (%). The ANOVA was used to identify the significance of the developed model in the RSM. Lastly, the RSM was used to optimize the adsorption results. The experimental results determined that the removal of 98.73 ± 1% of ACN (the highest amount) was achieved at pH 7, while the removal of 88.44 ± 1% of ACN (the lowest amount) was achieved at pH 4.5. The adsorption efficiency of ACN was slightly increased by an increase in activated carbon dosage from 0.1 to 3 g/L (<4%). The contact time was the most significant factor in controlling the adsorption efficiency of ACN in wastewater, and not pH value or dosage. The adsorption reaction was quick, and 88–90% of ACN was removed within 5 min of the adsorption reaction, followed by slower adsorption for up to 90 min. The RSM model was developed on the basis of experimental results. An ANOVA determined that the developed model was significant enough to represent the adsorption data as the p-value was <0.05 for the model. The factors pH, adsorbent dosage, and contact time were also significant factors (p-value < 0.05). The optimization results showed that pH of 6.96, adsorbent dosage of 2.62 g/L, and contact time of 71.67 min were the optimal conditions for eliminating 98.88% of the ACN. The optimization results were verified in the lab, and a close agreement was found between the predicted results of the RSM and experimental results. The study found that the RSM is an effective tool for optimizing operating variables, as well as for significantly reducing time and experimentation costs.
Impact of COVID-19 Pandemic on the Educational-Instructional Process of the Students from Technical Faculties
Authors: Spunei Elisabeta , Frumușanu Nătălița-Mihaela , Muntean Roxana , Mărginean Gabriela
Published: July 2022

Even though we live in a period when the word digitization is prevalent in many social areas, the COVID-19 pandemic has divided mankind into two main categories: some people have seen this crisis as an opportunity to move the activities online and, furthermore, to accelerate digitization in as many areas as possible, while others have been reluctant, keeping their preferences for face-to-face activities. The current work presents the results of an analysis on 249 students from 11 engineering faculties. The study aims to identify the impact of the COVID-19 pandemic on students’ educational experiences when switching from face-to-face to online education during a public health emergency or COVID-19-related state of alert. The overall conclusion was that, although the pandemic has brought adverse consequences on the health and life quality of many people, the challenges that humankind has been subjected to have led to personal and professional development and have opened up new perspectives for carrying out the everyday activities.
Impacts of Fracture Roughness and Near-Wellbore Tortuosity on Proppant Transport within Hydraulic Fractures
Authors: Wang Di , Bai Bingyang , Wang Bin , Wei Dongya , Liang Tianbo
Published: July 2022

For unconventional reservoir hydraulic fracturing design, a greater fracture length is a prime factor to optimize. However, the core observation results from the Hydraulic Fracturing Test Site (HFTS) show that the propped fractures are far less or shorter than expected, which suggests that the roughness and tortuosity of hydraulic fractures are crucial to sand transport. In this study, a transport model of sands is first built based on experimental measurements on the height and transport velocity of the sand bank in fractures with predetermined width and roughness. The fracture roughness is quantified by using the surface height integral. Then, three-dimensional simulations are conducted with this modified model to further investigate the impact of tortuous fractures on sand transport, from which a regression model is established to estimate the propped length of hydraulic fractures at a certain pumping condition. The experiment results show that the height of the sand bank in rough fractures is 20–50% higher than that in smooth fractures. The height of the sand bank decreases with the reduction in slurry velocity and increases with the increase in sand diameter. Sand sizes do little effect on the transport velocity of the sand bank, but the increase in slurry velocity and sand volume fraction can dramatically enhance the migration velocity of the sand bank. The appearance of tortuous fractures decreases the horizontal velocity of suspended particles and results in a higher sand bank compared with that in straight fractures. When the sand bank reaches equilibrium at the tortuous position, it is easy to produce vortices. So, there is a significant height of sand bank change at the tortuous position. Moreover, sand plugging can occur at the entrance of the fractures, making it difficult for the sand to transport deep into fractures. This study explains why the propped length of fractures in HFTS is short and provides a regression model that can be easily embedded in the fracturing simulation to quickly calculate dimensions of the propped fractures network to predict the length and height of propped fractures during fracturing.
Responses of Vegetation Autumn Phenology to Climatic Factors in Northern China
Authors: Li Zhaozhe , Wang Ranghui , Liu Bo , Qian Zhonghua , Wu Yongping , Li Cheng
Published: July 2022

Understanding the dynamics of vegetation autumn phenology (i.e., the end of growing season, EOS) is crucial for evaluating impacts of climate change on vegetation growth. Nevertheless, responses of the EOS to climatic factors were unclear at the regional scale. In this study, northern China was chosen for our analysis, which is a typical ecologically fragile area. Using the Enhanced Vegetation Index (EVI) and climatic data from 1982 to 2016, we extracted the EOS and analyzed its trends in northern China by using the linear least-squares regression and the Bayesian change-point detection method. Furthermore, the partial correlation analysis and multivariate regression analysis were used to determine which climatic factor was more influential on EOS. The main findings were as follows: (1) multi-year average of EOS mainly varied between 275 and 305 day of year (DOY) and had complicated spatial differences for different vegetation types; (2) the percentage of the pixel showing delaying EOS (65.50%) was larger than that showing advancing EOS (34.50%), with a significant delaying trend of 0.21 days/year at the regional scale during the study period. As for different vegetation types, their EOS trends were similar in sign but different in magnitude; (3) temperature showed a dominant role in governing EOS trends from 1982 to 2016. The increase in minimum temperature led to the delayed EOS, whereas the increase in maximum temperature reversed the EOS trends. In addition to temperature, the impacts of precipitation and radiation on EOS trends were more complex and largely depended on the vegetation types. These findings can provide a crucial support for developing vegetation dynamics models in northern China.
A Study of Contact Pressure with Thermo-Mechanical Coupled Action for a Full-Dimensional PEMFC Stack
Authors: Zhang Zhiming , Zhang Jun , Shi Liang , Zhang Tong
Published: July 2022

The contact pressure between bipolar plates (BPPs) and a membrane electrode assembly (MEA) has a key impact on Proton Exchange Membrane Fuel Cell (PEMFC) performance. However, it is difficult to obtain the contact pressure combined with operating temperature action via the finite element analysis (FEA) model, resulting in limited calculation resources for the problem of multiscale and thermo-mechanical coupled action in a full-dimensional fuel cell stack. This paper establishes an equivalent stiffness model for contact pressure, which could be predicted simply and quickly compared with the FEA model. Then, this presented model is validated by experimentation with a full-dimensional fuel cell stack assembled with 10 cells using pressure-sensitive film. The error between the presented model and the experimentation of the full-dimensional stack is a maximum of 4.41%. This work provides important insight into thermo-mechanical coupled action, as less empirical testing is required to identify the contact pressure in a full-dimensional fuel cell stack.
Reply to Thomas, K. Comment on “Hossain et al. Exposure to Dodecamethylcyclohexasiloxane (D6) Affects the Antioxidant Response and Gene Expression of Procambarus clarkii. Sustainability 2021, 13, 3495”
Authors: Hossain Md Muzammel , Tu Shuxin
Published: July 2022

We have reviewed and studied Karluss T.’s comment [...]
Gill Junction Injury and Microbial Disorders Induced by Microcystin-Leucine Arginine in Lithobates catesbeianus Tadpoles
Authors: Jiang Huiling , He Jun , Wang Hui , Zheng Lingling , Wang Xiaoran , Zhang Huijuan , Wu Hailong , Shu Yilin
Published: July 2022

Microcystin-LR (MC-LR) is widely present in waters around the world, but its potential toxic effects and mechanisms on amphibian gills remain unknown. In the present study, tadpoles (Lithobates catesbeianus) were exposed to environmentally realistic concentrations of 0.5, 2 μg/L MC-LR, and 0 μg/L MC-LR (Control) for 30 days with the objective to unveil the impairment of gill health. The lysozyme was downregulated, while pattern recognition receptors and complement and adaptive immune processes were upregulated and the ability of gill supernatant to inhibit pathogenic bacteria decreased in the 0.5 and 2 μg/L MC-LR groups. The transcriptions of epithelial barrier components (e.g., CLDN1) were significantly decreased in MC-LR-exposed gills, while the gill content of lipopolysaccharide (LPS) endotoxins and the transcriptions of downstream responsive genes (e.g., TLR4 and NF-κB) were concurrently increased. In addition, the number of eosinophils and the expression of pro-inflammatory cytokines (e.g., IL-1β and TNF-α) were increased. These results imply that exposure of tadpoles to low environmentally concentrations of MC-LR leads to inflammation, increased permeability, and a reduced ability to inhibit pathogenic bacteria. The epithelial cells of inner gill filaments increased and transcriptions of hypoxic stress genes (e.g., HIF-1α, FLT1, and SERPINE1) were upregulated within the exposed group. As a consequence, exposure to MC-LR may lead to hypoxic stress. MC-LR exposure also drove gill microbiota to a dysbiosis. The relative abundance of Elizabethkingia was positively correlated with content of LPS and transcriptions of NF-κB and TNF-α. Overall, this study presents the first evidence about the pronounced impacts of MC-LR exposure on gills of amphibians, highlighting the susceptibility of early developing tadpoles to the environmental risks of MC-LR.
Impact of Cyanotoxin Ingestion on Liver Cancer Development Using an At-Risk Two-Staged Model of Mouse Hepatocarcinogenesis
Authors: Mrdjen Igor , Lee Jiyoung , Weghorst Christopher M. , Knobloch Thomas J.
Published: July 2022

Exposure to cyanobacterial hepatotoxins has been linked to the promotion and increased incidence of liver cancer in pre-clinical and epidemiologic studies. The family of hepatotoxins, microcystins (MCs), are produced by over 40 cyanobacterial species found in harmful algal blooms (HABs) worldwide, with MC-LR being the most common and potent MC congener. In the current study, we hypothesized that the low-dose chronic ingestion of Microcystis cyanotoxins via drinking water would promote liver carcinogenesis in pre-initiated mice. Four groups of C3H/HeJ mice received one intraperitoneal (i.p.) injection of diethylnitrosamine (DEN) at 4 weeks of age. Three weeks later, the mice were administered ad libitum drinking water containing one of the following: (1) reverse osmosis, deionized water; (2) water containing 500 mg/L phenobarbital (PB500); (3) water with purified MC-LR (10 µg/L) added; or (4) water containing lysed Microcystis aeruginosa (lysate; 10 µg/L total MCs). The exposure concentrations were based on environmentally relevant concentrations and previously established Ohio EPA recreational water MC guidelines. Throughout the 30-week exposure, mouse weights, food consumption, and water consumption were not significantly impacted by toxin ingestion. We found no significant differences in the number of gross and histopathologic liver lesion counts across the treatment groups, but we did note that the PB500 group developed lesion densities too numerous to count. Additionally, the proportion of lesions classified as hepatocellular carcinomas in the MC-LR group (44.5%; p < 0.05) and lysate group (55%; p < 0.01) was significantly higher compared to the control group (14.9%). Over the course of the study, the mice ingesting the lysate also had a significantly lower survival probability (64.4%; p < 0.001) compared to water (96.8%), PB500 (95.0%), and MC-LR (95.7%) exposures. Using cyanotoxin levels at common recreational water concentration levels, we demonstrate the cancer-promoting effects of a single cyanotoxin MC congener (MC-LR). Furthermore, we show enhanced hepatocarcinogenesis and significant mortality associated with combinatorial exposure to the multiple MCs and bioactive compounds present in lysed cyanobacterial cells—a scenario representative of the ingestion exposure route, such as HAB-contaminated water and food.
Stromal Interaction Molecule 1 Promotes the Replication of vvIBDV by Mobilizing Ca2+ in the ER
Authors: Yan Nana , Wang Yongqiang , Chen Zehua , Liu Aijing , Li Yue , Yang Bo , Li Kai , Qi Xiaole , Gao Yulong , Gao Li , Liu Changjun , Zhang Yanping , Cui Hongyu , Pan Qing , Wang Xiaomei
Published: July 2022

Infectious bursal disease virus (IBDV) is one of the main threats to the poultry industry worldwide. Very virulent IBDV (vvIBDV) is a fatal virus strain that causes heavy mortality in young chicken flocks. Ca2+ is one of the most universal and versatile signalling molecules and is involved in almost every aspect of cellular processes. Clinical examination showed that one of the characteristics of vvIBDV-infected chickens was severe metabolic disorders, and the chemical examination showed that their serum Ca2+ level decreased significantly. However, there are limited studies on how vvIBDV infection modulates the cellular Ca2+ level and the effect of Ca2+ level changes on vvIBDV replication. In our study, we found Ca2+ levels in the endoplasmic reticulum (ER) of vvIBDV-infected B cells were higher than that of mock-infected cells, and the expression level of stromal interaction molecule 1 (STIM1), an ER Ca2+ sensor, was significantly upregulated due to vvIBDV infection. The knock-down expression of STIM1 led to decreased Ca2+ level in the ER and suppressed vvIBDV replication, while the over-expressed STIM1 led to ER Ca2+ upregulation and promoted vvIBDV replication. We also showed that the inhibition of Ca2+-release-activated-Ca2+ (CRAC) channels could reduce vvIBDV infection by blocking Ca2+ from entering the ER. This study suggests a new mechanism that STIM1 promotes the replication of vvIBDV by mobilizing Ca2+ in the ER.