bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Gut Bacteria in Enhancing Host Adaptation of Tuta absoluta to Different Host Plants
Authors: Shang Luo-Hua , Cai Xiang-Yun , Li Xiu-Jie , Wang Yu-Zhou , Wang Jin-Da , Hou You-Ming
Published: October 2024

The insect gut bacteria play important roles in insect development and growth, such as immune defense, nutrient metabolism, regulating insect adaptations for plants, etc. The Tuta absoluta (Meyrick) is a destructive invasive pest that mainly feeds on solanaceae plants. However, the relationship between gut microflora and host adaption of T. absoluta remains to be known. In this study, we first compared the survival adaptability of T. absoluta feeding with two host plants (tomatoes and potatoes). The T. absoluta completed the generation cycle by feeding on the leaves of both plants. However, the larvae feeding on tomato leaves have shorter larvae durations, longer adult durations, and a greater number of egg production per female. After Single Molecular Real-Time (SMRT) sequencing, according to the LDA Effect Size (LEfSe) analysis, the gut bacterial biomarker of T. absoluta fed on tomato was Enterobacter cloacae and the gut bacterial biomarker of T. absoluta fed on potatoes was Staphylococcus gallinarum and Enterococcus gallinarum. Furthermore, a total of 6 and 7 culturable bacteria were isolated from the guts of tomato- and potato-treated T. absoluta, respectively. However, the isolated strains included bacterial biomarkers E. cloacae and S. gallinarum but not E. gallinarum. In addition, different stains bacterial biomarkers on T. absoluta feeding selection were also studied. E. cloacae enhanced the host preference of the SLTA (T. absoluta of tomato strain) for tomato but had no impact on STTA (T. absoluta of potato strain). S. gallinarum improved the host preference of STTA to a potato but did not affect SLTA. The results showed that the gut bacteria of T. absoluta were affected by exposure to different host plants, and the bacterial biomarkers played an important role in host adaptability. This study not only deepens our understanding of gut bacteria-mediated insect-plant interactions but also provides theoretical support for the development of environmentally friendly and effective agricultural pest control methods.
Functional Response of Four Phytoseiid Mites to Eggs and First-Instar Larvae of Western Flower Thrips, Frankliniella occidentalis
Authors: Nguyen Viet Ha , Song Ziwei , Nguyen Duc Tung , Van Leeuwen Thomas , De Clercq Patrick
Published: October 2024

The predation capacity and functional responses of adult females of the phytoseiid mites Amblyseius largoensis (Muma), Proprioseiopsis lenis (Corpuz and Rimando), Paraphytoseius cracentis (Corpuz and Rimando), and Amblyseius swirskii (Athias-Henriot) were studied on eggs and first instars of the western flower thrips, Frankliniella occidentalis (Pergande), in the laboratory at 25 °C and 30 °C. At both temperatures, the functional response of all four phytoseiid mites was type II to first instars of the thrips. In contrast, when offered thrips eggs, the functional response was type III. At both temperatures tested, A. swirskii had the highest mean daily consumption of first-instar F. occidentalis, followed by A. largoensis, P. cracentis, and P. lenis. Amblyseius largoensis had the shortest handling time and the highest maximum attack rate when first-instar thrips were the prey. When fed on thrips eggs, A. largoensis had the highest mean daily consumption, followed by A. swirskii, P. cracentis, and P. lenis. On thrips eggs, A. swirskii showed the shortest handling time and highest maximum attack rate. Our findings indicate that all four phytoseiids had a better ability to prey on first-instar larvae of F. occidentalis compared to thrips eggs. At 25 and 30 °C, A. largoensis was the better predator on thrips larvae, whereas A. swirskii was superior in consuming eggs of F. occidentalis. Proprioseiopsis lenis was the inferior predator on both thrips larvae and eggs compared to the other phytoseiids tested.
Role of Liquid-Based Cytology in the Endoscopic Diagnosis of Pancreatic Ductal Adenocarcinoma
Authors: Kitagawa Koh , Mitoro Akira , Suzuki Hisae , Tomooka Fumimasa , Asada Shohei , Hanatani Jun-Ichi , Motokawa Yuki , Iwata Tomihiro , Osaki Yui , Takeda Maiko , Yoshiji Hitoshi
Published: October 2024

Recently, endoscopic ultrasound-guided tissue acquisition (EUS-TA) has been widely used to diagnose pancreatic ductal adenocarcinoma (PDAC). The histological examination of core tissues acquired using novel biopsy needles is the primary diagnostic approach for patients with PDAC. However, in patients with early-stage PDAC, such as Stages 0 and I, EUS-TA can be challenging, and its diagnostic accuracy may be limited. This presents a clinical dilemma: The earlier that clinicians attempt to accurately diagnose PDAC, the more difficult it becomes to do so using EUS-TA. Liquid-based cytology (LBC) is a technique for preparing pathological specimens from liquefied cytology specimens by placing the collected material in a special fixative preservative fluid. LBC offers advantages, such as specimen optimization with reduced blood interference, a high cell-collection rate, and the simplicity of the procedure in the endoscopy room. The use of LBC may improve diagnostic accuracy, particularly for early-stage PDAC. Therefore, we emphasize that cytology remains a valuable tool for the endoscopic diagnosis of PDAC. In this review, we discuss the role of LBC in the endoscopic diagnosis of PDAC.
Disparities in Heart Failure Deaths among Patients with Cirrhosis
Authors: Grobman Benjamin , Mansur Arian , Lu Christine Y.
Published: October 2024

Background: Heart failure deaths have increased in recent years in the United States and are projected to continue to increase in the future. Rates of liver disease and cirrhosis have similarly increased in the United States. Patients with cirrhosis are at an elevated risk of heart failure with a worsened prognosis. As such, investigations of the epidemiology of these comorbid conditions are important. Methods: We obtained data on heart failure deaths among people with cirrhosis in the United States from 1999 to 2020 from the Centers for Disease Control Wide-ranging Online Data for Epidemiologic Research multiple cause of death database. Rates were analyzed for the population as a whole and for demographic subgroups. Results: From 1999 to 2020, there were 7424 cirrhosis-related heart failure deaths. Rates were higher among Black (AAMR ratio = 1.288, 95% CI: 1.282–1.295) and Asian people (AAMR ratio = 3.310, 95% CI: 3.297–3.323) compared to White people. Rates were also higher in rural areas than in urban areas (AAMR ratio = 1.266, 95% CI: 1.261–1.271). Rates increased over time across demographic subgroups. Conclusions: People with cirrhosis are at an elevated risk of heart failure death compared to the general population. Rates were particularly elevated in Asian people, Black people, males, and people living in rural areas. These data indicate a significant and previously underappreciated disease burden. Clinicians taking care of cirrhosis patients should be aware of the risk of heart failure and should collaborate with cardiac specialists as needed.
An Update on Advances in Hypopituitarism: Etiology, Diagnosis, and Current Management
Authors: Iglesias Pedro
Published: October 2024

This article provides an updated review of hypopituitarism (HP), an endocrine disorder characterized by a deficiency of one or more pituitary hormones. The various etiologies are reviewed, including pituitary neuroendocrine tumors (PitNETs), hypothalamic lesions, genetic mutations, and acquired factors such as head trauma, medications, neoplasms, and infiltrative diseases. It is noted that PitNETs are responsible for approximately half of the cases in adults, whereas in children the causes are predominantly congenital. Diagnosis is based on clinical evaluation and hormonal testing, with identification of the specific hormonal deficiencies essential for effective treatment. Laboratory tests present challenges and limitations that must be understood and addressed. Hormone replacement therapy is the mainstay of treatment, significantly improving patients’ quality of life. It is important to know the possible interactions between hormone replacement therapies in HP. Recent advances in understanding the pathophysiology of HP and the importance of a multidisciplinary approach to the management of associated complications are discussed. This article emphasizes the need for comprehensive evaluation and continuous follow-up to optimize outcomes in patients with HP and highlights the importance of ongoing research to improve diagnostic and treatment strategies.
An Exploratory Study on the Effects of Souchard Postural Gymnastics in Parkinson’s Disease Patients with Camptocormia: A Quasi-Experimental Approach
Authors: Amadio Emanuele , Mencio Matteo , Carlizza Alessandra , Panuccio Francescaroberta , Sellitto Giovanni , Ruotolo Ilaria , Simeon Rachele , Berardi Anna , Galeoto Giovanni
Published: October 2024

Background/Objective: Parkinson’s disease (PD), a prevalent neurodegenerative disorder, leads to motor and non-motor impairments, affecting quality of life. Camptocormia can be one of the motor signs of PD, characterized by a severe and abnormal forward flexion of the thoracolumbar spine that typically occurs when walking or standing. The following study aims to verify whether postural gymnastics can be an effective treatment for trunk control, balance, activities of daily living, and general well-being in patients with early-stage PD and camptocormia. Methods: Nine participants (mean age 67.7 ± 7.8) with early PD (Hoehn and Yahr Scale ≤ 2) received 10 biweekly physiotherapy sessions. Outcomes were measured using the Parkinson’s Disease Questionnaire (PDQ-39) and Berg Balance Scale (BBS) along with trunk mobility and muscle tests according to the Medical Research Council (MRC) scale. Results: Statistically significant results were noted in the PDQ-39 mobility, ADLs and emotional well-being subscales and in the BBS; statistically significant improvements were also seen in trunk mobility and muscle strength. Conclusions: This study shows that the postural gymnastic treatment, according to Souchard, in patients with PD’s camptocormia has obtained good results and has the potential timprove mobility and balance, encouraging and motivating patients in their rehabilitation journeys.
Clinical Meaningfulness of an Algorithm-Based Service for Analyzing Treatment Response in Patients with Metastatic Cancer Using FDG PET/CT
Authors: Bupathi Manojkumar , Garmezy Benjamin , Lattanzi Michael , Kieler Minnie , Ibrahim Nevein , Perk Timothy G. , Weisman Amy J. , Perlman Scott B.
Published: October 2024

Background/Objectives: Determining how a patient with metastatic cancer is responding to therapy can be difficult for medical oncologists, especially with text-only radiology reports. In this investigation, we assess the clinical usefulness of a new algorithm-based analysis that provides spatial location and quantification for each detected lesion region of interest (ROI) and compare it to information included in radiology reports in the United States. Methods: Treatment response radiology reports for FDG PET/CT scans were retrospectively gathered from 228 patients with metastatic cancers. Each radiology report was assessed for the presence of both qualitative and quantitative information. A subset of patients (N = 103) was further analyzed using an algorithm-based service that provides the clinician with comprehensive quantitative information, including change over time, of all detected ROI with visualization of anatomical location. For each patient, three medical oncologists from different practices independently rated the usefulness of the additional analysis overall and in four subcategories. Results: In the 228 radiology reports, quantitative information of size and uptake was provided for at least one lesion at one time point in 78% (size) and 95% (uptake) of patients. This information was reported for both analyzed time points (current scan and previous comparator) in 52% (size) and 66% (uptake) of patients. Only 7% of reports quantified the total number of lesions, and none of the reports quantified changes in all lesions for patients with more than a few lesions. In the assessment of the augmentative algorithm-based analysis, the majority of oncologists rated it as overall useful for 98% of patients (101/103). Within specific categories of use, the majority of oncologists voted to use it for making decisions regarding systemic therapy in 97% of patients, for targeted therapy decisions in 72% of patients, for spatial location information in 96% of patients, and for patient education purposes in 93% of patients. Conclusions: For patients with metastatic cancer, the algorithm-based analysis of all ROI would allow oncologists to better understand treatment response and support their work to more precisely optimize the patient’s therapy.
PLGA/Ti-Zn as Nanocomposite for Drug Delivery of Oleoresin
Authors: Rodríguez-Barajas Noé , Martin-Camacho Ubaldo de Jesús , Salazar-Mendoza Jasmin , Ghotekar Suresh , Sánchez-Burgos Jorge Alberto , González-Vargas Oscar Arturo , Fellah Mamoun , Macías-Carballo Monserrat , Gutiérrez-Mercado Yanet Karina , Camargo-Hernández Gabriela , Rodríguez-Razón Christian Martin , Pérez-Larios Alejandro
Published: October 2024

Capsicum annuum L. var. “Chile de árbol” combined with poly(lactic-co-glycolic acid) (PLGA) and TiO2-ZnO oxides synthesized at different molar ratios and pH (Ti-Zn A and B 3:1, 1:1, and 1:3) via the sol-gel method was characterized by the Brunauer–Emmett–Teller (BET) method, a UV-Vis spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), High-Performance Liquid Chromatography (HPLC-DAD), and a release profile through mathematical models to explain its behavior. Furthermore, FTIR revealed the presence of PLGA, TiO2, and ZnO as well as amino group characteristics from oleoresin components, principally alkaloid groups (capsaicin and dihydrocapsaicin), as evidenced by HPLC, to identify the presence of capsaicin and dihydrocapsaicin. The UV-Vis spectra showed a slight hypsochromic shift in the PLGA treatments. The release profile demonstrated a higher controllable release in the PLGA treatments than in the double nanoemulsions. Moreover, it is important to note that the effect of NPs influenced the release profile itself, increasing the release when NPs were synthesized at an acidic pH. Therefore, the TiZnOl/PLGA A characteristics suggest that these results have potential for pharmaceutical (as drug carriers) and medical applications.
Computational Analysis of Stiffness Reduction Effects on the Dynamic Behaviour of Floating Offshore Wind Turbine Blades
Authors: O. Aikhuele Daniel , E. Diemuodeke Ogheneruona
Published: October 2024

This paper describes the study of a floating offshore wind turbine (FOWT) blade in terms of its dynamic response due to structural damage and its repercussions on structural health monitoring (SHM) systems. Using a finite element model, natural frequencies and mode shapes were derived for both an undamaged and a damaged blade configuration. A 35% reduction in stiffness at node 1 was applied in order to simulate significant damage. Concretely, the results are that the intact blade has a fundamental frequency of 0.16 Hz, and this does not change when damaged, while higher modes exhibit frequency changes: mode 2 drops from 2.05 Hz to 2.00 Hz and mode 3 from 6.15 Hz to 6.01 Hz. The shifts show a critical loss in the capability of handling vibrational energy due to the damage; higher modes (4, 5, and 6) show larger frequency deviations going down to as low as 18.06 Hz in mode 6. The mode shape change is considerable for the edge-wise and flap-wise deflection of the 2D contour plots, indicating possible coupling effects between modes. These results indicate that lower modes are sensitive to stiffness reductions, and the continuous monitoring of the lower harmonic modes early is required to detect damages. These studies have helped to improve blade design, maintenance, and operational safety for FOWT systems.
Molecular Mechanism of Mok I Gene Overexpression in Enhancing Monacolin K Production in Monascus pilosus
Authors: Huang Zhiwei , Xiao Lishi , Mo Wenlan , Zhang Yaru , Cai Yiyang , Huang Simei , Chen Zhiting , Long Chuannan
Published: October 2024

Monascus species are capable of producing various active metabolites, including monacolin K (MK) and pigments. Studies have shown that the overexpression of the mok I gene from the MK synthesis gene cluster in Monascus species can significantly increase MK production; however, the molecular mechanism has not yet been fully elucidated. Therefore, this study focused on the mok I gene of Monascus pilosus to construct overexpression strains of the mok I gene, resulting in high-yield MK production. Sixteen positive transformants were obtained, seven of which produced 9.63% to 41.39% more MK than the original strain, with no citrinin detected in any of the transformants. The qRT-PCR results revealed that the expression levels of mok I in the transformed strains TI-13, TI-24, and TI-25 increased by more than 50% compared to the original strain at various fermentation times, with the highest increase being 10.9-fold. Furthermore, multi-omics techniques were used to analyze the molecular mechanisms underlying enhanced MK production in transformed strains. The results indicated that mok I overexpression may enhance MK synthesis in M. pilosus by regulating the expression of key genes (such as MAO, HPD, ACX, and PLC) and the synthesis levels of key metabolites (such as delta-tocopherol and alpha-linolenic acid) in pathways linked to the biosynthesis of cofactors, the biosynthesis of unsaturated fatty acids, tyrosine metabolism, ubiquinone and other terpenoid-quinone biosynthesis, alpha-linolenic acid metabolism, and glycerophospholipid metabolism. These findings provide a theoretical basis for further study of the metabolic regulation of MK in Monascus species and for effectively enhancing their MK production.
Connections and Spatial Network Structure of the Tourism Economy in Beijing–Tianjin–Hebei: A Social Network Perspective
Authors: Zhang Xiaoyuan , Huang Xiankai , Shi Jinlian , Zheng Yaomin , Wang Jiahong
Published: October 2024

In the context of the coordinated development of Beijing–Tianjin–Hebei, examining the spatial connections and network structure characteristics of the tourism economy holds substantial theoretical and practical significance. This study constructs a tourism economic quality evaluation index system from four dimensions and optimizes the tourism economic gravity model by incorporating time distance and weight factors to dynamically simulate the intensity of tourism economic spatial connection (ITESC). Based on the new perspective of relational data, social network analysis is employed to investigate the spatial correlation network characteristics and group structure changes in the tourism economy within the Beijing–Tianjin–Hebei urban agglomeration (BTHUA). The results demonstrate a significant improvement in overall tourism economic quality from 2013 to 2022, with the average quality increasing from 0.128 in 2013 to 0.157 in 2022. Tourism economic spatial connections are prevalent among cities, exhibiting heterogeneous characteristics, characterized by density in the north and relatively sparse in the south. The network cohesion of ITESC strengthens, and the network structure evolves from a highly centralized to a multi-centralized configuration, with increasingly frequent cooperation and interaction in tourism between cities. Beijing, Tianjin, and Shijiazhuang occupy central positions in the network, exhibiting strong ITESC and a high degree of connectivity, generating a significant “trickle-down effect” on peripheral cities. Consequently, strengthening ITESC between peripheral cities and core areas, as well as enhancing the bidirectional spillover of tourism development between cities, is crucial for the future sustainable and coordinated development of tourism in the BTHUA.
Land Space and High-Speed Transportation Coordinated Development Evaluation in the Beijing–Tianjin–Hebei Urban Agglomeration of China
Authors: Lu Yujin , Xu Xingmeng , Zhu Gaoru , Peng Yuting , Li Yi , Zhao Xueyan
Published: October 2024

There is a complex interaction between national land space and transportation. China is accelerating its strategy of building a strong transportation country and territorial spatial planning, which requires evaluating and strengthening the coordination relationship between transport and land space to promote development. Taking the Beijing–Tianjin–Hebei urban agglomeration in China as an example, this study analyze the high-speed transportation construction and land space development process, evaluates the coupling and coordination degree between high-speed transportation and land space, as well as the “development-protection” situation in the years 2000, 2010, and 2020 by means of the coupling and coordination model, spatial autocorrelation analysis, and GIS analysis. The result shows: (1) high-speed transportation construction and territorial space development have been active for a long time, and most of the cities have maintained a high level of increase in these two indicators. In terms of protection indicators, Tangshan and other coastal cities have been lagging behind for a long time without any improvement trend. (2) The coupling and coordination level between high-speed transportation and development is high, with slight weakening over time, with an average annual decrease of less than 0.09%. The change in the coupling coordination level with protection shows the wave dynamics of ‘low-middle-low’. (3) In the future, high-speed transportation will have a significant impact on land space in the country for a long time. In this regard, it is suggested to: (1) promote the construction of high-speed transportation for network, systematic, and serial development; (2) accelerate the extension of the high-speed transportation advantages of the existing growth poles of Beijing and Tianjin; and (3) develop Shijiazhuang and Langfang as the new growth poles. This study will promote the integration of transportation and urban planning, which will contribute to the development of high-quality synergistic urban agglomerations around the world.
Neighbourhood Density in Spoken Word Recognition: An Eye-Tracking Study
Authors: Schelletter Christina
Published: October 2024

Bilingual acquisition research has so far focused on comparisons with monolingual children in terms of linguistic features, performance on standardised tests, input, etc. In contrast, new methodologies, such as eye-tracking, can offer a more detailed understanding of the way bilinguals use both language systems. Eye fixations provide evidence of online processing. This study investigates spoken word recognition in children and adults. Word retrieval is affected by the number of words that minimally differ from the target (neighbourhood). Previous research found that only bilingual adults activated a similar-sounding competitor from the other language. As children have been found to be sensitive to neighbourhood density from quite early in previous research, similar results might be expected. This study includes 56 subjects (11 German–English bilingual and 12 English monolingual children, aged 8; 21 English monolingual and 12 German–English bilingual adults). The subjects’ online processing of competing items for high- and low-density targets was compared. The results confirm that neighbourhood density affects word recognition in adults and children. The bilingual children activated same-language competitors as well as similar-sounding competitors from the other language. Adults and children differed in terms of latencies, and the monolinguals were more accurate than the bilingual subjects.
Omega-3 and Sports: Focus on Inflammation
Authors: Cannataro Roberto , Abrego-Guandique Diana Marisol , Straface Natascia , Cione Erika
Published: October 2024

Inflammation is expected in sports, especially when practiced at a high level. The human body is pushed toward its limit, and this is perceived as a “stressogenic agent”. Athletes, especially elite ones, desire it because their bodies can react with super-compensation, i.e., improve muscle mass, strength, speed, resistance, and, therefore, athletic performance. Thus, the inflammatory stimuli should be there during training but also counteracted to have the body placed in the optimal conditions for reacting with super-compensation. In this sense, omega-3 fatty acids have been shown to have anti-inflammatory biochemical activity. In this review, we will present the biochemical mechanisms of action of omega-3 fatty acids through their mediators, specialized pro-resolving mediators, which have anti-inflammatory activity. A focus will be on studies on omega-3 fatty acid supplementation in sports, and we will provide indications for possible practical applications and future studies, which are undoubtedly necessary to clarify the omega-3 fatty acids used in sports practice.
Editorial for Special Issue of Motion Planning and Advanced Control for Robotics
Authors: Crespo Jonathan , Barber Ramon
Published: October 2024

Advancements in robotics are increasingly essential in addressing complex challenges associated with motion planning and control, particularly in environments that require physical interaction with various elements [...]
Weak Quasi-Contact Metric Manifolds and New Characteristics of K-Contact and Sasakian Manifolds
Authors: Rovenski Vladimir
Published: October 2024

Quasi-contact metric manifolds (introduced by Y. Tashiro and then studied by several authors) are a natural extension of contact metric manifolds. Weak almost-contact metric manifolds, i.e., where the linear complex structure on the contact distribution is replaced by a nonsingular skew-symmetric tensor, have been defined by the author and R. Wolak. In this paper, we study a weak analogue of quasi-contact metric manifolds. Our main results generalize some well-known theorems and provide new criterions for K-contact and Sasakian manifolds in terms of conditions on the curvature tensor and other geometric objects associated with the weak quasi-contact metric structure.
Factors Influencing the Clinical Course of Bullous Pemphigoid among Geriatric Patients: A Pilot Study
Authors: Mazan Paula , Lesiak Aleksandra , Bednarski Igor , Sobolewska-Sztychny Dorota , Noweta Marcin , Narbutt Joanna
Published: October 2024

Background and Objectives: Bullous pemphigoid (BP) is the most common autoimmune blistering disease affecting mainly elderly patients. Still, little is known about the pathogenesis of pruritus in BP or the factors that affect the clinical course of the disease. This study aimed to evaluate the factors influencing the clinical course of BP among older patients. Materials and Methods: A retrospective analysis of medical records of 55 patients with BP hospitalized in the dermatology department in 2015–2021 was conducted. The study focused on preliminary diagnosis, medical history, clinical examination (characteristics and location of cutaneous changes), laboratory investigation, and direct and indirect immunofluorescence. Results: Analysis of laboratory results in combination with the clinical course of BP showed that red blood cell count, hemoglobin, and hematocrit values were negatively associated with a risk of erosions and erythema, while MCHC values were positively correlated with a risk of associated pruritus. A correlation was found between neurological diseases and an increased risk of erosions. Conclusions: We have shown that age and neurological conditions, including stroke, affect the clinical course of BP. Further studies on a larger group of patients should be conducted to investigate the different factors affecting the clinical aspect of BP and to understand the relationship between them.
Exploring the Role of Hemogram-Derived Ratios and Liver Fibrosis Scores in Pulmonary Fibrosis
Authors: Ciornolutchii Vera , Ruta Victoria Maria , Man Adina Milena , Motoc Nicoleta Stefania , Popa Stefan-Lucian , Dumitrascu Dan L. , Ismaiel Abdulrahman , Leucuta Daniel-Corneliu
Published: October 2024

Background and Objectives: Pulmonary fibrosis, including idiopathic pulmonary fibrosis (IPF) and secondary pulmonary fibrosis (SPF), is a progressive lung disease that significantly impairs respiratory function. Accurate differentiation between IPF and SPF is crucial for effective management. This study explores the association between pulmonary fibrosis and hepatic conditions, evaluating the utility of various hemogram-derived ratios and hepatic fibrosis scores in distinguishing between IPF and SPF. Materials and Methods: We conducted a retrospective study involving patients diagnosed with IPF or SPF at the “Leon Daniello” Clinical Hospital of Pneumology in Cluj-Napoca, Romania. Pulmonary fibrosis was confirmed via imaging techniques, and hepatic steatosis and fibrosis were assessed using non-invasive scores. We analyzed clinical, laboratory, and pulmonary function data, focusing on hemogram-derived ratios and hepatic scores. Statistical analyses, including ROC curves, were used to evaluate the effectiveness of these biomarkers in differentiating IPF from SPF. Results: We included a total of 38 patients with IPF and 28 patients with SPF. Our findings revealed that IPF patients had a significantly higher FIB-4 score compared to SPF patients, suggesting increased hepatic fibrosis risk in IPF, as well as an increased RDW/PLT ratio. Conversely, SPF patients exhibited elevated PLR, PNR, and SII, reflecting a more pronounced inflammatory profile. PLR and PNR demonstrated the highest discriminatory ability between IPF and SPF, while traditional hepatic fibrosis scores showed limited differentiation capabilities. No significant differences in pulmonary function tests were observed across hepatic fibrosis risk categories. Conclusions: The study highlights the value of biomarkers like PLR and PNR in differentiating between IPF and SPF, offering additional diagnostic insights beyond traditional imaging. Integrating hepatic assessments into the management of pulmonary fibrosis could improve diagnostic accuracy and patient care.
Ti-Ta-Cu Biocompatible Alloy System Development via Selective Laser Melting for Prosthetic Applications
Authors: Polozov Igor , Sokolova Victoria , Gracheva Anna , Zolotarev Anton , Nefyodova Victoria , Popovich Anatoly
Published: October 2024

This study investigated the development of Ti-Ta-Cu alloys via selective laser melting (SLM) for potential prosthetic applications. Ti-Ta-Cu alloys with 10, 15, and 20 wt.% Ta were fabricated using in situ alloying of elemental powders. We examined the effects of Ta content and SLM processing parameters on microstructure, phase composition, mechanical properties, and corrosion resistance. X-ray diffraction analysis revealed an increase in β-phase content with increasing Ta concentration. Microstructural analysis showed a dendritic structure in Ta-rich areas, with remelting strategies improving chemical homogeneity and Ta dissolution. The Ti-20Ta-5Cu alloy exhibited the best balance of strength and ductility, with an ultimate tensile strength of 1011 MPa and elongation of 5.7%. All compositions demonstrated lower elastic moduli (103–109 GPa) compared to traditional titanium alloys. Microhardness values were highest for Ti-15Ta-5Cu, ranging from 359 to 410 HV0.5 depending on SLM parameters. Corrosion testing in Hank’s solution showed improved pitting resistance for Ti-15Ta-5Cu and Ti-20Ta-5Cu compared to Ti-10Ta-5Cu. The study demonstrates the feasibility of producing Ti-Ta-Cu alloys with tailored properties via SLM, offering potential for customized prosthetic applications with improved biomechanical compatibility and functionality.
Mitochondrial Abundance and Function Differ Across Muscle Within Species
Authors: Yen Con-Ning , Bodmer Jocelyn S. , Wicks Jordan C. , Zumbaugh Morgan D. , Persia Michael E. , Shi Tim H. , Gerrard David E.
Published: October 2024

Background: Mitochondria are considered the powerhouse of cells, and skeletal muscle cells are no exception. However, information regarding muscle mitochondria from different species is limited. Methods: Different muscles from cattle, pigs and chickens were analyzed for mitochondrial DNA (mtDNA), protein and oxygen consumption. Results: Bovine oxidative muscle mitochondria contain greater mtDNA (p < 0.05), protein (succinate dehydrogenase, SDHA, p < 0.01; citrate synthase, CS, p < 0.01; complex I, CI, p < 0.05), and oxygen consumption (p < 0.01) than their glycolytic counterpart. Likewise, porcine oxidative muscle contains greater mtDNA (p < 0.01), mitochondrial proteins (SDHA, p < 0.05; CS, p < 0.001; CI, p < 0.01) and oxidative phosphorylation capacity (OXPHOS, p < 0.05) in comparison to glycolytic muscle. However, avian oxidative skeletal muscle showed no differences in absolute mtDNA, SDHA, CI, complex II, lactate dehydrogenase, or glyceraldehyde 3 phosphate dehydrogenase compared to their glycolytic counterpart. Even so, avian mitochondria isolated from oxidative muscles had greater OXPHOS capacity (p < 0.05) than glycolytic muscle. Conclusions: These data show avian mitochondria function is independent of absolute mtDNA content and protein abundance, and argue that multiple levels of inquiry are warranted to determine the wholistic role of mitochondria in skeletal muscle.
Liraglutide Therapy in Obese Patients Alters Macrophage Phenotype and Decreases Their Tumor Necrosis Factor Alpha Release and Oxidative Stress Markers—A Pilot Study
Authors: Bułdak Łukasz , Bołdys Aleksandra , Skudrzyk Estera , Machnik Grzegorz , Okopień Bogusław
Published: October 2024

Introduction: Obesity is one of the major healthcare challenges. It affects one in eight people around the world and leads to several comorbidities, including type 2 diabetes, hyperlipidemia, and arterial hypertension. GLP-1 analogs have become major players in the therapy of obesity, leading to significant weight loss in patients. However, benefits resulting from their usage seem to be greater than simple appetite reduction and glucose-lowering potential. Recent data show better cardiovascular outcomes, which are connected with the improvements in the course of atherosclerosis. Macrophages are crucial cells in the forming and progression of atherosclerotic lesions. Previously, it was shown that in vitro treatment with GLP-1 analogs can affect macrophage phenotype, but there is a paucity of in vivo data. Objective: To evaluate the influence of in vivo treatment with liraglutide on basic phenotypic and functional markers of macrophages. Methods: Basic phenotypic features were assessed (including inducible nitric oxide synthase, arginase 1 and mannose receptors), proinflammatory cytokine (IL-1β, TNFα) release, and oxidative stress markers (reactive oxygen species, malondialdehyde) in macrophages obtained prior and after 3-month therapy with liraglutide in patients with obesity. Results: Three-month treatment with subcutaneous liraglutide resulted in the alteration of macrophage phenotype toward alternative activation (M2) with accompanying reduction in the TNFα release and diminished oxidative stress markers. Conclusions: Our results show that macrophages in patients treated with GLP-1 can alter their phenotype and function. Those findings may at least partly explain the pleiotropic beneficial cardiovascular effects seen in subjects treated with GLP-1 analogs.
Legionella in Primary School Hot Water Systems from Two Municipalities in the Danish Capital Region
Authors: Nielsen Niss Skov , Fojan Peter , Jensen Rasmus Lund , Wahedi Haseebullah , Afshari Alireza
Published: October 2024

Legionella contamination in public water systems poses significant health risks, particularly in schools where vulnerable populations, including children, regularly use these facilities. This study investigates the presence of Legionella in the hot water systems from 49 primary schools across two municipalities in the Danish capital region. Water samples were collected from taps in each school, and both first-flush and stabile temperature samples were analysed for Legionella contents. The findings revealed that 97% of schools in Municipality 1 and 100% in Municipality 2 had Legionella in their hot water systems. The content of Legionella colonies was significantly higher in schools in Municipality 1, which was probably because of overall lower water temperatures. At stabile temperatures, 76% and 50% of the schools in the two municipalities exceeded the European Union’s recommended limit of 1000 CFU/L. Stabile peripheral water temperatures were achieved after 3 min. Tap water temperatures above 54 °C and central tank temperatures above 59 °C were associated with Legionella contents below 1000 CFU/L. This study highlights the need for more stringent Legionella control procedures in schools, including higher water temperatures and refining Legionella reducing interventions with the addition of regular flow and draining procedures.
Applying Subcritical Water Extraction to Obtain Bioactive Compounds and Cellulose Fibers from Brewer Spent Grains
Authors: Gomez-Contreras Paula Andrea , Obando Catalina , Freitas Pedro Augusto Vieira de , Martin-Perez Laia , Chiralt Amparo , Gonzalez-Martinez Chelo
Published: October 2024

Of the three types of waste generated in beer processing, brewer’s spent grain (BSG) is the most abundant and has a high potential for valorization. In this work, defatted BSG (DB) was subjected to an extraction process with subcritical water at different temperatures to obtain extracts rich in phenols and the cellulosic fractions, which were also purified by using hydrogen peroxide (H2O2). The results showed that the dry extracts obtained at 170 °C were richer in phenolics (24 mg Gallic Acid Equivalent (GAE) g−1 DB), but with lower antioxidant capacity (71 mg DB·mg−1 2,2-diphenyl-1-pikryl-hydrazyl). This extract also showed the highest antibacterial potential against L. innocua (80 mg·mL−1) and E. coli (140 mg·mL−1) than those obtained at lower temperatures. The purification of cellulose from the treated residues, using hydrogen peroxide, revealed that DB is a limited source of cellulose material since the bleached fractions showed low yields (20–25%) and low cellulose purity (42–71%), even after four bleaching cycles (1 h) at pH 12 and 8% H2O2. Despite this, the subcritical water extraction method highlights the potential of a simple process as a technological option to convert underutilized side streams like beer bagasse into added-value, potential ingredients for innovative food and pharmaceutical applications.
Influence of Electrostatic Field on Optical Rotation of D-Glucose Solution: Experimental Research for Electric Field-Induced Biological Effect
Authors: Guo Quanlin , Gou Dezhi , Zhao Chenxi , Ma Yun , Chen Chaojun , Zhu Junxi
Published: October 2024

At present, the effects of environmental electromagnetic irradiation on the metabolism of organisms have attracted extensive attention, but the mechanism is still not clear. D-glucose plays an important role in the metabolism of organisms. In this work, the change in the optical rotation of D-glucose solution under an electrostatic field is measured experimentally, so as to explain the mechanism of the electric field-induced biological effect. The experimental results show that the electrostatic field can alter the optical rotation of D-glucose solution at different temperatures. Under the different strengths of electrostatic field, the specific rotation of D-glucose solution increases at different temperatures; the maximum increase can reach 2.07%, but the effect of temperature and electric field strength on the rotation increment is nonlinear and very complex. Further, it turns out that the proportion of α-D-glucose in solution increases by up to 3.25% under the electrostatic field, while the proportion of β-D-glucose decreases by as much as 1.75%. The experimental study confirms that electrostatic field can change the proportion of two conformation molecules (α and β-D-glucose) in D-glucose solution, which can provide a novel explanation for the mechanism of the electric field-induced biological effect.
Synthesis, Urease Inhibition, Molecular Docking, and Optical Analysis of a Symmetrical Schiff Base and Its Selected Metal Complexes
Authors: Bonne Samuel , Saleem Muhammad , Hanif Muhammad , Najjar Joseph , Khan Salahuddin , Zeeshan Muhammad , Tahir Tehreem , Ali Anser , Lu Changrui , Chen Ting
Published: October 2024

Designing and developing small organic molecules for use as urease inhibitors is challenging due to the need for ecosystem sustainability and the requirement to prevent health risks related to the human stomach and urinary tract. Moreover, imaging analysis is widely utilized for tracking infections in intracellular and in vivo systems, which requires drug molecules with emissive potential, specifically in the low-energy region. This study comprises the synthesis of a Schiff base ligand and its selected transition metals to evaluate their UV/fluorescence properties, inhibitory activity against urease, and molecular docking. Screening of the symmetrical cage-like ligand and its metal complexes with various eco-friendly transition metals revealed significant urease inhibition potential. The IC50 value of the ligand for urease inhibition was 21.80 ± 1.88 µM, comparable to that of thiourea. Notably, upon coordination with transition metals, the ligand–nickel and ligand–copper complexes exhibited even greater potency than the reference compound, with IC50 values of 11.8 ± 1.14 and 9.31 ± 1.31 µM, respectively. The ligand–cobalt complex exhibited an enzyme inhibitory potential comparable with thiourea, while the zinc and iron complexes demonstrated the least activity, which might be due to weaker interactions with the investigated protein. Meanwhile, all the metal complexes demonstrated a pronounced optical response, which could be utilized for fluorescence-guided targeted drug delivery applications in the future. Molecular docking analysis and IC50 values from in vitro urease inhibition screening showed a trend of increasing activity from compounds 7d to 7c to 7b. Enzyme kinetics studies using the Lineweaver–Burk plot indicated mixed-type inhibition against 7c and non-competitive inhibition against 7d.
Erinacenones A–L: Twelve New Isoindolinone Alkaloids from the Edible and Medicinal Mushroom Hericium erinaceus
Authors: Yuan Lin-Lin , Liu Ji-Kai
Published: October 2024

A total of twelve previously unreported isoindolin-1-one compounds, erinacenones A–L (1–12), were isolated from liquid cultures of the medicinal fungus Hericium erinaceus. Their structures were elucidated based on spectroscopic data analysis. The absolute configuration of 12 was determined by comparing its optical rotations with values reported in the literature. The most distinctive feature of these compounds is that their nitrogen atoms are connected to different parts of the special structure moieties. Among them, compounds 3 and 4, as well as 10 and 11, are two pairs of isomers differing only by a small change in the position of one double bond. Compounds 4 and 5 were found to show cytotoxic activities, with IC50 values of 24.7 and 18.4 μM, respectively, against MCF-7 cell lines.
The Inhibitory Effect of Agastache rugosa Essential Oil on the Dental Biofilm
Authors: Kim Eun Sook , Park Bog-Im , Kim Young-Hoi , Kang Jooyi , You Yong-Ouk
Published: October 2024

This study aimed to identify the inhibitory effect of Agastache rugosa essential oil (AREO) on the cariogenic properties of Streptococcus mutans, which causes dental caries and dental plaque formation. After extracting the AREO, their effects on the growth and acid production of S. mutans were examined. Furthermore, S. mutans biofilm formation was observed on the resin teeth surface. The effect on the expression of biofilm-related genes of S. mutans was measured using real-time PCR. AREO components were analyzed using gas chromatography (GC) and GC-mass spectrometry (MS). The growth and acid production of S. mutans were significantly inhibited at concentrations of 0.02 mg/mL or higher of AREO. At 0.04 mg/mL, inhibition was similar to that of the positive control, 0.1% NaF. AREO suppressed the expression of virulence factors such as gtfB, gtfC, gtfD, gbpB, SpaP, brpA, relA, and vicR at concentrations of 0.02 mg/mL or higher. As a result of GC and GC-MS analyses, the main components of AREO included estragole, limonene, and β-caryophyllene. These results suggest that A. rugosa may be a useful agent for inhibiting the cariogenic properties of S. mutans.
Validity and Reliability According to the Type of Examiners in the Process of Calibrating Dental Caries Experience Using the DMFT Index
Authors: Fernández-Coll Anna Paola , Garcés-Elías María Claudia , Beltrán Jorge A. , León-Manco Roberto A. , Mas-López Janett
Published: October 2024

The process of examiner calibration is an essential step in all epidemiological research, as it aims to ensure uniform interpretation, understanding, and application of the instrument to be used. This ensures that the data collected will be valid and reliable. This study aimed to determine the differences in concordance in dental caries calibration across three dental specialties. The population consisted of 45 dentists, divided into three groups: 15 general dentists working in the public sector, 15 dentists specializing in Dental Public Health, and 15 dentists specializing in Restorative and Aesthetic Dentistry. The calibration process was carried out in three stages: theory, calibration using photographs, and calibration on natural teeth, performed by the gold standard. In the first validity process, a statistical difference was only found between the Kappa values of the inter-examiner calibration process using photographs. For the evaluation of teeth, in the second validity process, 33.33% (n = 15) of the participants achieved “almost perfect agreement.” Finally, only 75.56% (n = 34) of the examiners were considered for the reliability report; of this group, 52.94% (n = 18) were in “almost perfect agreement,” and 35.29% (n = 12) were in “substantial agreement.” The validity and reliability of the dental caries experience calibration process did not present significant statistical differences between general dentists in the public sector, dentists specializing in Dental Public Health, and dentists specializing in Restorative and Aesthetic Dentistry.
Impact of Metaverse Technology on Academic Achievement and Motivation in Middle School Science
Authors: Al-Muqbil Norah Saleh Mohamed
Published: October 2024

This study explores the effects of Metaverse technology on middle school learners’ academic performance and motivation in science subjects. Utilizing a quasi-experimental design, 33 students in the experimental group were exposed to the Metaverse for one semester, while 32 students in the control group continued with traditional teaching methods at School 148 in Riyadh. Data collection instruments included a validated science achievement test and a motivation scale. The results demonstrated that the nodes were statistically significantly improved in the experimental group, receiving an average post-test score of 73.1, as compared with the control group, receiving an average post-test score of 65.9 (t = 2.3, p < 0.05). The scores in motivation were also slightly higher in the experimental group, with a mean of 26.9, as compared with the control group, with a mean of 17.1 (t = 5.75, p < 0.05). For academic achievement and motivation, the effect sizes were quite high: fixed effect = 1.091; random effect equals 1.086. These results demonstrate the possibilities of Metaverse technology in revolutionizing the way students learn science. This technology could be a valuable tool for instruction in science classes to enhance performances and influence students’ attitudes positively towards enhanced learning environments in schools.