bluuebunny/crossref_metadata_2025 huggingface dataset:
Temporal Relationship between Daily Reports of COVID-19 Infections and Related GDELT and Tweet Mentions
Authors: Owuor Innocensia , Hochmair Hartwig H.
Published: September 2023

Social media platforms are valuable data sources in the study of public reactions to events such as natural disasters and epidemics. This research assesses for selected countries around the globe the time lag between daily reports of COVID-19 cases and GDELT (Global Database of Events, Language, and Tone) and Twitter (X) COVID-19 mentions between February 2020 and April 2021 using time series analysis. Results show that GDELT articles and tweets preceded COVID-19 infections in Australia, Brazil, France, Greece, India, Italy, the U.S., Canada, Germany, and the U.K., while for Poland and the Philippines, tweets preceded and GDELT articles lagged behind COVID-19 disease incidences, respectively. This shows that the application of social media and news data for surveillance and management of pandemics needs to be assessed on a case-by-case basis for different countries. It also points towards the applicability of time series data analysis for only a limited number of countries due to strict data requirements (e.g., stationarity). A deviation from generally observed lag patterns in a country, i.e., periods with low COVID-19 infections but unusually high numbers of COVID-19-related GDELT articles or tweets, signals an anomaly. We use the seasonal hybrid extreme Studentized deviate test to detect such anomalies. This is followed by text analysis of news headlines from NewsBank and Google on the date of these anomalies to determine the probable event causing an anomaly, which includes elections, holidays, and protests.
Geomorphological Mapping Global Trends and Applications
Authors: Quesada-Román Adolfo , Peralta-Reyes Manuel
Published: September 2023

This study conducts a bibliometric analysis of 735 research papers on geomorphological mapping published in English between 2000 and 2021 using the Web of Science database. The analysis focuses on key metrics such as annual publication rates, journal distribution, common keywords, and frequently cited papers. The results demonstrate sustained investment in geomorphological mapping research over the past two decades, driven by advancements in data analysis, GIS technologies, and cross-institutional and cross-country collaboration. While European universities and research centers lead the field, researchers from Latin America and Asia are also making noteworthy contributions. However, research concentration remains largely in Europe, particularly at low altitudes. The study highlights the vital importance of investment in geomorphological mapping research and the benefits of collaboration to advance understanding and knowledge production. It also emphasizes the need for greater geographic and cultural diversity among researchers to ensure a more comprehensive and inclusive approach to research in this field.
Permafrost and Gas Hydrate Response to Ground Warming
Authors: Chuvilin Evgeny , Sokolova Natalia
Published: September 2023

This Special Issue of Geosciences is a collection of fifteen original research and overview papers on the response of permafrost and gas hydrates to ground warming caused by natural climate trends and industrial loads [...]
‘Together We Prepare a Feast, Each Person Stirring Up Memory’
Authors: Stevens Ed , Khlusova Anna , Fine Sarah , Azzouz Ammar , Ansems de Vries Leonie
Published: September 2023

Our story starts in April 2020, in the early stages of the UK’s first national COVID-19 lockdown. A multidisciplinary team of researchers and artists began a collaboration with Migrateful, a charity that runs cookery classes led by refugees, asylum seekers, and migrants struggling to integrate and access employment. Teaching classes and sharing their cuisine and stories helps the chefs develop their confidence and sense of belonging, and food is central to the enterprise. The focus of the project was a series of interactive online cookery classes delivered by Migrateful chefs, with ongoing involvement from the researchers and artists. In this paper, we weave together the research team’s reflections on the project with commentary from the participants and artists. We outline our methods and our learning from the collaboration and explain how it inspired new ways of thinking about refugee representation, food and belonging, co-creative storytelling, and virtual engagement. We discuss the ways in which Migrateful’s model helps to support the production of counter-narratives that value, foreground, and amplify migrants’ perspectives and voices while acknowledging the tensions involved in adapting this model to the virtual space. We emphasise the power dynamics inherent in engaging and researching with marginalised people and their stories while considering whether artistic involvement and creation may help to navigate some of these challenges, and we address how the virtual environment affected the potential for collaborative storytelling, interaction, and engagement levels among participants. Together, these reflections form a ‘recipe’ for what we hope to be a more meaningful and ethical model of engagement activity that builds on this learning.
Prefrontal Cortex Activation during Memory Training by Virtual Drum Beating: A Randomized Controlled Trial
Authors: Nam Yeon-Gyo , Kwon Bum-Sun
Published: September 2023

The use of virtual reality (VR) content in neurological disorders with cognitive impairment is increasing. We have developed a device that incorporates virtual drum beating content, designed for digit memorization training. This study aimed to investigate the effects of realistic cognitive training on brain activity using functional near-infrared spectroscopy (fNIRS). Thirty healthy individuals were recruited and randomly assigned into two groups: conventional cognitive exercise (CCE) and a realistic cognitive exergame (RCE). Subjects in the CCE group underwent memory training by memorizing numbers displayed on a computer screen and then writing them on paper. The main outcome measure was the oxyhemoglobin level in the dorsolateral prefrontal cortex (DLPFC). As a result, the average number of digits was 7.86 ± 0.63 for the CCE and 7.6 ± 0.82 for the RCE. The mean difference in ΔHbO was 1.417 ± 0.616 μm (p = 0.029) in channel 2, located in the right DLPFC. Channel 7 and channel 10, which measured activations in the hypothesized medial orbitofrontal cortex (OFC), also showed a significant mean difference of ΔHbO. DLPFC and OFC presented higher activation in the RCE group (p < 0.05), attributable to the simultaneous memory training and virtual drum beating, which provided various sensory inputs (visual, auditory, and vibration). Although DLPFC involvement in cognitive processes remains controversial, our findings suggest that realistic memory training using drumming content can lead to safer activation of the DLPFC compared to conventional cognitive training.
A Nutritional Supplement as Adjuvant of Gabapentinoids for Adults with Neuropathic Pain following Spinal Cord Injury and Stroke: Preliminary Results
Authors: Raffaeli William , Felzani Giorgio , Tenti Michael , Greco Luca , D’Eramo Maria Pia , Proietti Stefania , Morone Giovanni
Published: September 2023

Gabapentinoids are first choice drugs for central neuropathic pain (CNP) despite limited evidence of efficacy and side effects affecting therapy outcomes. Nutraceuticals could improve their efficacy and tolerability. Our aim is to investigate the effect of NACVAN®, in addition to gabapentinoids, on pain symptomatology in CNP patients. The effect of 6 weeks of treatment of NACVAN® was preliminary observed among 29 adult inpatients with spinal cord injury (SCI) or stroke-related CNP recruited to the experimental group. Pain intensity, neuropathic pain, and quality-of-life were measured at baseline (T0) and after 3 (T1) and 6 weeks (T2). Change in each outcome over time was assessed through a repeated measures analysis of variance or Wilcoxon matched-pairs test. Preliminary results show a significant reduction in pain intensity (T0 → T1, p = 0.021; T0 → T2, p = 0.011; T1 → T2, p = 0.46), neuropathic symptoms (T0 → T1, p = 0.024; T0 → T2, p = 0.003), and evoked pain (T0 → T2, p = 0.048). There were no significant reductions in other neuropathic pain dimensions and in quality-of-life components. No side-effects were detected. NACVAN® could have a beneficial adjuvant effect when used as an add-on to gabapentinoids in patients suffering from CNP due to SCI or stroke, with no adverse effect. Future analysis on a larger sample, compared with a placebo condition, could confirm these preliminary results.
Effect of Conservative Rehabilitation Interventions on Health-Related Quality of Life in Women with Upper Limb Lymphedema Secondary to Breast Cancer: A Systematic Review
Authors: Muñoz-Alcaraz María Nieves , Jiménez-Vílchez Antonio José , Pérula-de Torres Luis Ángel , Serrano-Merino Jesús , García-Bustillo Álvaro , Pardo-Hernández Rocío , González-Bernal Jerónimo Javier , González-Santos Josefa
Published: September 2023

Breast cancer-related lymphedema (BCRL) of the upper limb is a very common condition in women undergoing breast cancer treatment; it can cause considerable alterations in the daily life of patients and a decrease in their health-related quality of life (HRQoL). Currently, there are many conservative therapies that try to palliate the symptoms, but the results are still controversial and there are still no globally accepted treatments. The purpose of this article is to determine the effect, according to the current available evidence, on HRQoL of different conservative interventions in the rehabilitation of BCRL in the upper limb in women. Eighteen articles that compared the effects of standard treatments, such as manual lymphatic drainage-based decongestive therapy or compression measures, and other newer treatments, including new technologies and other types of treatment programs, were reviewed. According to the results of this review, the most recommended modality for the improvement of HRQoL would be a complex decongestive technique without manual lymphatic drainage. Although there are clinical trials that have demonstrated the effectiveness of various treatments, the results of the positive effects on HRQoL remain highly controversial. There is a need to continue to develop studies to help guide therapeutic decisions that can promote HRQoL in women affected by upper limb BCRL.
Learning Spaces in Higher Education: A State-of-the-Art Review
Authors: Papaioannou Grigorios , Volakaki Maria-Garyfallio , Kokolakis Spyridon , Vouyioukas Demosthenes
Published: September 2023

Educational learning spaces encompass a spectrum, from traditional classrooms to contemporary online platforms and immersive virtual reality settings, fostering versatile teaching methods like collaboration, project-based, and experiential learning. Our study delves into modern higher education environments, particularly the shift from conventional to innovative spaces. Our focus centers on the benefits and challenges intrinsic to these emerging learning spaces, intending to enlighten educators, policymakers, and researchers. Our exploration commences by revealing the limitations entrenched within traditional learning environments, emphasizing the vital need for inventive solutions to meet evolving educational demands. We further investigate diverse learning scenarios, ranging from hybrid and remote setups to the integration of online platforms and virtual tools. Through this lens we navigate complexities introduced by these novel modalities, including potential reductions in face-to-face interactions and heightened demand for adept instructional and technological support. Lastly, our inquiry underscores the disparities between traditional and contemporary learning spaces, accentuating the potential for innovative settings to elevate higher education quality. Here, we illuminate the anticipated merits of such spaces, notably heightened student engagement, enriched collaboration, and amplified creativity. Concurrently, we explore technology’s pivotal role in shaping learning environments and ultimately influencing pedagogical methodologies. Our future research will explore how Artificial Intelligence can improve higher education.
Design of Innovative Clothing for Pressure Injury Prevention: End-User Evaluation in a Mixed-Methods Study
Authors: Salgueiro-Oliveira Anabela , Rêgo Anderson da Silva , Santos-Costa Paulo , Bernardes Rafael A. , Filipe Luísa , Sousa Liliana B. , Barboza Rochelne , Carvalho Miguel , Bouçanova Maria , Lopes Maria Clara Ferreira da Graça , Apóstolo João A. , Parreira Pedro
Published: September 2023

The global relevance of pressure injury (PI) prevention technologies arise from their impact on the quality of life of people with limited mobility and the costs associated with treating these preventable injuries. The purpose of this mixed methods study is to evaluate the design of a prototype integrating Smart Health Textiles for PI prevention based on feedback from specialist nurses who care for individuals who are prone to or have PIs. This is a mixed methods study. A structured questionnaire was conducted as part of an evaluation of a prototype garment for the prevention of PIs. This questionnaire was applied during the evaluation of the prototype and afterwards focus group discussions were held with experts. Descriptive statistics techniques were used to analyze the data and thematic and integrated content analysis was conducted through concomitant triangulation. Nineteen nurses took part, aged 30 to 39 years (52.6%) and with 12.31 ± 8.96 years of experience. Participants showed that the prototype required more manipulation and physical effort, which interfered its usefulness, in addition to presenting difficulties with the openings and the material of the closure system, which interfered with the ease of use and learning. Overall satisfaction with the product was moderate, with some areas for improvement found, such as satisfaction, recommendations to colleagues, and pleasantness of use. It is concluded that areas for improvement have been found in all dimensions, including in the design of openings and the choice of materials. These findings supply significant insights for improving clothing to meet the needs of healthcare professionals and patients.
Moderating Effects of Emotional Recognition Competency in Rejective Parenting and Adolescent Depression and Aggression
Authors: Shin Jaeeun , Bae Sung Man
Published: September 2023

Rejective parenting is a major antecedent of emotional instability and aggressive behavioral problems. Previous studies have reported that emotional problems, such as depression, anxiety, aggression, and conduct problems in children and adolescents, improve through interventions that enhance emotional recognition competency. In this study, we explored whether the emotional recognition ability levels of individual adolescents moderated the pathway of negative parenting on aggression mediated by depression The moderated mediating effect of emotional recognition competency was investigated through examining 2265 first-year high school students using the 2021 data from the Korean Children and Youth Panel Survey 2018. There was no significant moderating effect on the direct pathway from rejective parenting to aggression. The moderating effect of emotional recognition competency on the indirect pathway leading to aggression through depression in rejective parenting was significant. These results suggest that the ability to correctly understand signals related to the emotions of others can play an important role in reducing depression and aggressive behavior by reducing conflict with people around them and experiencing more support.
A Wandering Detection Method Based on Processing GPS Trajectories Using the Wavelet Packet Decomposition Transform for People with Cognitive Impairment
Authors: Jafarpournaser Naghmeh , Delavar Mahmoud Reza , Noroozian Maryam
Published: September 2023

The increasing prevalence of cognitive disorders among the elderly is a significant consequence of the global aging phenomenon. Wandering stands out as the most prominent and challenging symptom in these patients, with potential irreversible consequences such as loss or even death. Thus, harnessing technological advancements to mitigate caregiving burdens and disease-related repercussions becomes paramount. Numerous studies have developed algorithms and smart healthcare and telemedicine systems for wandering detection. Broadly, these algorithms fall into two categories: those estimating path complexity and those relying on historical trajectory data. However, motion signal processing methods are rarely employed in this context. This paper proposes a motion-signal-processing-based algorithm utilizing the wavelet packet transform (WPT) with a fourth-order Coiflet mother wavelet. The algorithm identifies wandering patterns solely based on patients’ positional data on the current traversed path and variations in wavelet coefficients within the frequency–time spectrum of motion signals. The model’s independence from prior motion behavior data enhances its compatibility with the pronounced instability often seen in these patients. A performance assessment of the proposed algorithm using the Geolife open-source dataset achieved accuracy, precision, specificity, recall, and F-score metrics of 83.06%, 92.62%, 83.06%, 83.06%, and 87.58%, respectively. Timely wandering detection not only prevents irreversible consequences but also serves as a potential indicator of progression to severe Alzheimer’s in patients with mild cognitive impairment, enabling timely interventions for preventing disease progression. This underscores the importance of advancing wandering detection algorithms.
T-Cadherin Deficiency Is Associated with Increased Blood Pressure after Physical Activity
Authors: Popov Vladimir S. , Brodsky Ilya B. , Balatskaya Maria N. , Balatskiy Alexander V. , Ozhimalov Ilia D. , Kulebyakina Maria A. , Semina Ekaterina V. , Arbatskiy Mikhail S. , Isakova Viktoria S. , Klimovich Polina S. , Sysoeva Veronika Y. , Kalinina Natalia I. , Tkachuk Vsevolod A. , Rubina Kseniya A.
Published: September 2023

T-cadherin is a regulator of blood vessel remodeling and angiogenesis, involved in adiponectin-mediated protective effects in the cardiovascular system and in skeletal muscles. GWAS study has previously demonstrated a SNP in the Cdh13 gene to be associated with hypertension. However, the role of T-cadherin in regulating blood pressure has not been experimentally elucidated. Herein, we generated Cdh13∆Exon3 mice lacking exon 3 in the Cdh13 gene and described their phenotype. Cdh13∆Exon3 mice exhibited normal gross morphology, life expectancy, and breeding capacity. Meanwhile, their body weight was considerably lower than of WT mice. When running on a treadmill, the time spent running and the distance covered by Cdh13∆Exon3 mice was similar to that of WT. The resting blood pressure in Cdh13∆Exon3 mice was slightly higher than in WT, however, upon intensive physical training their systolic blood pressure was significantly elevated. While adiponectin content in the myocardium of Cdh13∆Exon3 and WT mice was within the same range, adiponectin plasma level was 4.37-fold higher in Cdh13∆Exon3 mice. Moreover, intensive physical training augmented the AMPK phosphorylation in the skeletal muscles and myocardium of Cdh13∆Exon3 mice as compared to WT. Our data highlight a critically important role of T-cadherin in regulation of blood pressure and stamina in mice, and may shed light on the pathogenesis of hypertension.
BrrA02.LMI1 Encodes a Homeobox Protein That Affects Leaf Margin Development in Brassica rapa
Authors: Li Pan , Wu Yudi , Han Xiangyang , Li Hui , Wang Limin , Chen Bin , Yu Shuancang , Wang Zheng
Published: September 2023

Leaf margin morphology is an important quality trait affecting the commodity and environmental adaptability of crops. Brassica rapa is an ideal research material for exploring the molecular mechanisms underlying leaf lobe development. Here, we identified BrrA02.LMI1 to be a promising gene underlying the QTL qBrrLLA02 controlling leaf lobe formation in B. rapa, which was detected in our previous study. Sequence comparison analysis showed that the promoter divergences were the most obvious variations of BrrA02.LMI1 between parental lines. The higher expression level and promoter activity of BrrA02.LMI1 in the lobe-leafed parent indicated that promoter variations of BrrA02.LMI1 were responsible for elevating expression and ultimately causing different allele effects. Histochemical GUS staining indicated that BrrA02.LMI1 is mainly expressed at the leaf margin, with the highest expression at the tip of each lobe. Subcellular localization results showed that BrrA02.LMI1 was in the nucleus. The ectopic expression of BrrA02.LMI1 in A. thaliana resulted in a deep leaf lobe in the wild-type plants, and lobed leaf formation was disturbed in BrrA02.LMI11-downregulated plants. Our findings revealed that BrrA02.LMI1 plays a vital role in regulating the formation of lobed leaves, providing a theoretical basis for the selection and breeding of leaf-shape-diverse varieties of B. rapa.
A Human Brain Model Mimicking Umbilical Cord Mesenchymal Stem Cells for the Treatment of Hypoxic-Ischemic Brain Injury
Authors: Li Xidan , Liu Haijing , Han Chao , Luo Jianglin , Guan Xin , Wang Liang , Li Ying , Wang Jiayi , Piao Hua , Zou Wei , Liu Jing
Published: September 2023

We used an in vitro model of the human brain immune microenvironment to simulate hypoxic-ischemic brain injury (HIBI) and treatment with human umbilical cord mesenchymal stem cells (hUMSCs) to address the transformation barriers of gene differences between animals and humans in preclinical research. A co-culture system, termed hNAME, consisted of human hippocampal neurons (N), astrocytes (A), microglia (M), and brain microvascular endothelial cells (E). Flow cytometry measured the apoptosis rates of neurons and endothelial cells. hNAME-neurons and endothelial cells experienced more severe damage than monolayer cells, particularly after 48 h and 24 h of reoxygenation (OGD48/R24). Western blotting identified neuroinflammatory response markers, including HIF-1α, C1q, C3, TNF-α, and iNOS. Inflammatory factors originated from the glial chamber rather than the neurons and vascular endothelial chambers. A gradual increase in the release of inflammatory factors was observed as the OGD and reoxygenation times increased, peaking at OGD48/R24. The hNAME value was confirmed in human umbilical cord mesenchymal stem cells (hUMSCs). Treatment with hUMSCs resulted in a notable decrease in the severity of neuronal and endothelial cell damage in hNAME. The hNAME is an ideal in vitro model for simulating the immune microenvironment of the human brain because of the interactions between neurons, vessels, astrocytes, and microglia.
Overexpression of the First Peanut-Susceptible Gene, AhS5H1 or AhS5H2, Enhanced Susceptibility to Pst DC3000 in Arabidopsis
Authors: Liang Bingbing , Bai Yuanjun , Zang Chaoqun , Pei Xue , Xie Jinhui , Lin Ying , Liu Xiaozhou , Ahsan Taswar , Liang Chunhao
Published: September 2023

Salicylic acid (SA) serves as a pivotal plant hormone involved in regulating plant defense mechanisms against biotic stresses, but the extent of its biological significance in relation to peanut resistance is currently lacking. This study elucidated the involvement of salicylic acid (SA) in conferring broad-spectrum disease resistance in peanuts through the experimental approach of inoculating SA-treated leaves. In several other plants, the salicylate hydroxylase genes are the typical susceptible genes (S genes). Here, we characterized two SA hydroxylase genes (AhS5H1 and AhS5H2) as the first S genes in peanut. Recombinant AhS5H proteins catalyzed SA in vitro, and showed SA 5-ydroxylase (S5H) activity. Overexpression of AhS5H1 or AhS5H2 decreased SA content and increased 2,5-DHBA levels in Arabidopsis, suggesting that both enzymes had a similar role in planta. Moreover, overexpression of each AhS5H gene increased susceptibility to Pst DC3000. Analysis of the transcript levels of defense-related genes indicated that the expression of AhS5H genes, AhNPR1 and AhPR10 was simultaneously induced by chitin. Overexpression of each AhS5H in Arabidopsis abolished the induction of AtPR1 or AtPR2 upon chitin treatment. Eventually, AhS5H2 expression levels were highly correlated with SA content in different tissues of peanut. Hence, the expression of AhS5H1 and AhS5H2 was tissue-specific.
Study on Spontaneous Reactivation and Aging of Acetylcholinesterase Inhibited by Paraoxon and Malaoxon in Ten Species
Authors: Gao Mingwei , Ni Zhongwen , Li Guo , Wu Gang , Huang Binbin
Published: September 2023

Organophosphorus insecticides (OPs), acting as serine phosphorylating agents in acetylcholinesterase (AChE), are highly effective neurotoxic insecticides. In our previous research, we found that six herbivorous pests and four ladybirds howed significantly higher AChE LC50 values than seven parasitoids and a predator (Epistrophe balteate), and that there was a significant correlation with the corresponding bimolecular rate constant (Ki) value. The Ki value of pests was much smaller than that of natural enemies and had a higher LC50 value.Then, we speculated that the low sensitivity of the pest AChE to OPs may be associated with its higher recovery and lower aging ability. In this work, the I50 and I90 were calculated, to determine the sensibility of AChE in ten representative species, including Plutella xylostella, Prodenia litura, Musca domestica, and Cavia porcellus, to paraoxon and malaoxon. The enzyme activities were measured at various time points, and kinetic calculations were used to obtain their spontaneous reactivation (Ks) and aging (Ka) constants, which were comprehensively compared. We conclude that the Ka and Ks of the AChE inhibited by OPs showed primarily species-specific correlations, and little correlation with the sensitivity to OPs. The differences in the AChE sensitivity to paraoxon among the ten species were much greater than in the sensitivity to malaoxon. Compared to paraoxon, malaoxon was more selective for Cavia porcellus. Coleoptera insects showed a stronger dephosphorylation ability than other insect groups. The recovery ability of phospho-AChE was stronger in mammals than in insects, which could be related to the low sensitivity of the AChE site of action to OPs. The Ka of the AChE inhibited by malaoxon was larger than that inhibited by paraoxon with the corresponding biomaterials, indicating that the OP type had a substantial relationship with the Ka of the AChE. We further discovered that, when insects were inhibited by OP, the tendency of AChE to undergo aging was greater than that of dephosphorylation. Overall, the study provides valuable information on the action mechanism of various OPs on AChE in several species, which could be used to further research into AChE and the potential dangers that organophosphates pose to animals.
Genome-Wide Analysis of WUSCHEL-Related Homeobox Gene Family in Sacred Lotus (Nelumbo nucifera)
Authors: Chen Gui-Zhen , Huang Jie , Lin Zhi-Cong , Wang Fei , Yang Song-Min , Jiang Xiao , Ahmad Sagheer , Zhou Yu-Zhen , Lan Siren , Liu Zhong-Jian , Peng Dong-Hui
Published: September 2023

WUSCHEL-related homeobox (WOX) is a plant-specific transcription factor (TF), which plays an essential role in the regulation of plant growth, development, and abiotic stress responses. However, little information is available on the specific roles of WOX TFs in sacred lotus (Nelumbo nucifera), which is a perennial aquatic plant with important edible, ornamental, and medicinal values. We identified 15 WOX TFs distributing on six chromosomes in the genome of N. nucifera. A total of 72 WOX genes from five species were divided into three clades and nine subclades based on the phylogenetic tree. NnWOXs in the same subclades had similar gene structures and conserved motifs. Cis-acting element analysis of the promoter regions of NnWOXs found many elements enriched in hormone induction, stress responses, and light responses, indicating their roles in growth and development. The Ka/Ks analysis showed that the WOX gene family had been intensely purified and selected in N. nucifera. The expression pattern analysis suggested that NnWOXs were involved in organ development and differentiation of N. nucifera. Furthermore, the protein–protein interaction analysis showed that NnWOXs might participate in the growth, development, and metabolic regulation of N. nucifera. Taken together, these findings laid a foundation for further analysis of NnWOX functions.
Presence of Activated (Phosphorylated) STAT3 in Radiation Necrosis Following Stereotactic Radiosurgery for Brain Metastases
Authors: Jablonska Paola Anna , Galán Nuria , Barranco Jennifer , Leon Sergio , Robledano Ramón , Echeveste José Ignacio , Calvo Alfonso , Aristu Javier , Serrano Diego
Published: September 2023

Brain radiation necrosis (RN) is a subacute or late adverse event following radiotherapy, involving an exacerbated inflammatory response of the brain tissue. The risk of symptomatic RN associated with stereotactic radiosurgery (SRS) as part of the treatment of brain metastases (BMs) has been a subject of recent investigation. The activation of the signal transducer and activator of transcription 3 (STAT3) was shown in reactive astrocytes (RA) associated with BMs. Given that the pathophysiological mechanisms behind RN are not fully understood, we sought to investigate the role of STAT3 among other inflammatory markers in RN development. A mouse model of RN using clinical LINAC-based SRS was designed to induce brain necrosis with the administration of 50 Gy in a single fraction to the left hemisphere using a circular collimator of 5 mm diameter. Immunohistochemistry and multiplex staining for CD4, CD8, CD68, GFAP, and STAT3 were performed. For validation, eleven patients with BMs treated with SRS who developed symptomatic RN and required surgery were identified to perform staining for CD68, GFAP, and STAT3. In the mouse model, the RN and perinecrotic areas showed significantly higher staining for F4/80+ and GFAP+ cells, with a high infiltration of CD4 and CD8 T-lymphocytes, when compared to the non-irradiated cerebral hemisphere. A high number of GFAP+pSTAT3+ and F4/80+pSTAT3+ cells was found in the RN areas and the rest of the irradiated hemisphere. The analysis of human brain specimens showed that astrocytes and microglia were actively phosphorylating STAT3 in the areas of RN and gliosis. Phosphorylated STAT3 is highly expressed in the microglia and RA pertaining to the areas of brain RN. Targeting STAT3 via inhibition represents a promising strategy to ameliorate symptomatic RN in BM patients undergoing SRS.
Identification of Candidate Biomarkers of Alzheimer’s Disease via Multiplex Cerebrospinal Fluid and Serum Proteomics
Authors: Liu Ping , Li Lingxiao , He Fangping , Meng Fanxia , Liu Xiaoyan , Su Yujie , Su Xinhui , Luo Benyan , Peng Guoping
Published: September 2023

Alzheimer’s disease (AD) is the most prevalent form of dementia among elderly people worldwide. Cerebrospinal fluid (CSF) is the optimal fluid source for AD biomarkers, while serum biomarkers are much more achievable. To search for novel diagnostic AD biomarkers, we performed a quantitative proteomic analysis of CSF and serum samples from AD and normal cognitive controls (NC). CSF and serum proteomes were analyzed via data-independent acquisition quantitative mass spectrometry. Our bioinformatic analysis was based on Gene Ontology (GO) functional annotation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. In comparison to the controls, 8 proteins were more abundant in AD CSF, and 60 were less abundant in AD CSF, whereas 55 proteins were more and 10 were less abundant in the serum samples. ATPase-associated activity for CSF and mitochondrial functions for CSF and serum were the most enriched GO terms of the DEPs. KEGG enrichment analysis showed that the most significant pathways for the differentially expressed proteins were the N-glycan biosynthesis pathways. The area under the curve (AUC) values for CSF sodium-/potassium-transporting ATPase subunit beta-1 (AT1B1), serglycin (SRGN), and thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3) were 0.867 (p = 0.004), 0.833 (p = 0.008), and 0.783 (p = 0.025), respectively. A panel of the above three CSF proteins accurately differentiated AD (AUC = 0.933, p = 0.001) from NC. The AUC values for serum probable phospholipid-transporting ATPase IM (AT8B4) and SRGN were moderate. The AUC of the CSF SRGN + serum SRGN was 0.842 (p = 0.007). These novel AD biomarker candidates are mainly associated with inflammation, ATPase activity, oxidative stress, and mitochondrial dysfunction. Further studies are needed to investigate the molecular mechanisms by which these potential biomarkers are involved in AD.
Analysis of the Potassium-Solubilizing Priestia megaterium Strain NK851 and Its Potassium Feldspar-Binding Proteins
Authors: Wu Xinyue , Zhao Zijian , Zhao Zirun , Zhang Youjun , Li Mingchun , Yu Qilin
Published: September 2023

Potassium-solubilizing bacteria are an important microbial group that play a critical role in releasing mineral potassium from potassium-containing minerals, e.g., potassium feldspar. Their application may reduce eutrophication caused by overused potassium fertilizers and facilitate plants to utilize environmental potassium. In this study, a high-efficiency potassium-solubilizing bacterium, named NK851, was isolated from the Astragalus sinicus rhizosphere soil. This bacterium can grow in the medium with potassium feldspar as the sole potassium source, releasing 157 mg/L and 222 mg/L potassium after 3 days and 5 days of incubation, respectively. 16S rDNA sequencing and cluster analysis showed that this strain belongs to Priestia megaterium. Genome sequencing further revealed that this strain has a genome length of 5,305,142 bp, encoding 5473 genes. Among them, abundant genes are related to potassium decomposition and utilization, e.g., the genes involved in adherence to mineral potassium, potassium release, and intracellular trafficking. Moreover, the strong potassium-releasing capacity of NK851 is not attributed to the acidic pH but is attributed to the extracellular potassium feldspar-binding proteins, such as the elongation factor TU and the enolase that contains potassium feldspar-binding cavities. This study provides new information for exploration of the bacterium-mediated potassium solubilization mechanisms.
Insights into Repeated Renal Injury Using RNA-Seq with Two New RPTEC Cell Lines
Authors: Merrick B. Alex , Martin Negin P. , Brooks Ashley M. , Foley Julie F. , Dunlap Paul E. , Ramaiahgari Sreenivasa , Fannin Rick D. , Gerrish Kevin E.
Published: September 2023

Renal proximal tubule epithelial cells (RPTECs) are a primary site for kidney injury. We created two RPTEC lines from CD-1 mice immortalized with hTERT (human telomerase reverse transcriptase) or SV40 LgT antigen (Simian Virus 40 Large T antigen). Our hypothesis was that low-level, repeated exposure to subcytotoxic levels of 0.25–2.5 μM cisplatin (CisPt) or 12.5–100 μM aflatoxin B1 (AFB1) would activate distinctive genes and pathways in these two differently immortalized cell lines. RNA-seq showed only LgT cells responded to AFB1 with 1139 differentially expressed genes (DEGs) at 72 h. The data suggested that AFB1 had direct nephrotoxic properties on the LgT cells. However, both the cell lines responded to 2.5 μM CisPt from 3 to 96 h expressing 2000–5000 total DEGs. For CisPt, the findings indicated a coordinated transcriptional program of injury signals and repair from the expression of immune receptors with cytokine and chemokine secretion for leukocyte recruitment; robust expression of synaptic and substrate adhesion molecules (SAMs) facilitating the expression of neural and hormonal receptors, ion channels/transporters, and trophic factors; and the expression of nephrogenesis transcription factors. Pathway analysis supported the concept of a renal repair transcriptome. In summary, these cell lines provide in vitro models for the improved understanding of repeated renal injury and repair mechanisms. High-throughput screening against toxicant libraries should provide a wider perspective of their capabilities in nephrotoxicity.
Unraveling the Role of Epithelial Cells in the Development of Chronic Rhinosinusitis
Authors: Ha Jong-Gyun , Cho Hyung-Ju
Published: September 2023

The pathophysiology of CRS is multifactorial and complex yet needs to be completed. Recent evidence emphasizes the crucial part played by epithelial cells in the development of CRS. The epithelial cells act as physical barriers and play crucial roles in host defense, including initiating and shaping innate and adaptive immune responses. This review aims to present a comprehensive understanding of the significance of nasal epithelial cells in CRS. New research suggests that epithelial dysfunction plays a role in developing CRS through multiple mechanisms. This refers to issues with a weakened barrier function, disrupted mucociliary clearance, and irregular immune responses. When the epithelial barrier is compromised, it can lead to the passage of pathogens and allergens, triggering inflammation in the body. Furthermore, impaired mucociliary clearance can accumulate pathogens and secretions of inflammatory mediators, promoting chronic inflammation. Epithelial cells can release cytokines and chemokines, which attract and activate immune cells. This can result in an imbalanced immune response that continues to cause inflammation. The interaction between nasal epithelial cells and various immune cells leads to the production of cytokines and chemokines, which can either increase or decrease inflammation. By comprehending the role of epithelial cells in CRS, we can enhance our understanding of the disease’s pathogenesis and explore new therapeutics.
The Antioxidant and Antimicrobial Activities of Two Sun-Dried Fig Varieties (Ficus carica L.) Produced in Eastern Morocco and the Investigation of Pomological, Colorimetric, and Phytochemical Characteristics for Improved Valorization
Authors: Tikent Aziz , Laaraj Salah , Marhri Ahmed , Taibi Mohamed , Elbouzidi Amine , Khalid Ibtissame , Bouhrim Mohamed , Elfazazi Kaoutar , Elamrani Ahmed , Addi Mohamed
Published: September 2023

The eastern region of Morocco is renowned for the production of two varieties of figs, Ghoudane (GD) and Chetoui (CH), which are characterized by their high productivity and quality. To ensure a profitable selling price, producers often dry these figs, a process that not only increases their storage capability but also enhances their nutritional and health benefits. The aim of this study was to investigate the composition and potential health benefits of dried GD and CH figs. The findings of this study reveal that both varieties of dried figs have a rich composition of sugars, lipids, proteins, vegetable fibers, vitamins, minerals, polyphenols, and other nutrients. Moreover, both GD and CH figs that have been sun-dried meet the United Nations Economic Commission for Europe’s standard in terms of dry matter and diameter. However, their marketability and commercial quality as dried figs are only classified as Category I or lower-caliber figs. In terms of color, CH figs are lighter and brighter than their GD counterparts, making them more appealing to consumers. Furthermore, this study investigated the extraction of polyphenols and flavonoids from both dried fig varieties using different solvents. Distilled water was found to be the best extraction solvent for polyphenols in both varieties, with GD figs showing a higher content (483.6 mg GAE/100 g) than CH figs (408.3 mg GAE/100 g). Meanwhile, ethanol was found to be the best extraction solvent for flavonoids, with GD figs (180.2 mg of QE/100 g) showing a higher content than CH figs (122.9 mg of QE/100 g). In addition, the majority of the extracts, particularly the ethanol extracts, showed high efficiency against the tested bacteria and yeast. Notably, dried GD figs had higher amounts of total phenolics, total flavonoids, and antioxidant and antimicrobial activities compared to dried CH figs. The study also revealed high correlations between phytochemical content and both antioxidant and antimicrobial activities. In conclusion, this study highlights the nutritional and health benefits of dried figs, particularly the GD variety, and their potential as a natural source of antimicrobial compounds. The findings suggest that dried figs could be an alternative source of natural antimicrobial agents for various applications in the food, pharmaceutical, and cosmetic industries.
A Deep Learning Approach for Predictive Healthcare Process Monitoring
Authors: Ramirez-Alcocer Ulises Manuel , Tello-Leal Edgar , Romero Gerardo , Macías-Hernández Bárbara A.
Published: September 2023

In this paper, we propose a deep learning-based approach to predict the next event in hospital organizational process models following the guidance of predictive process mining. This method provides value for the planning and allocating of resources since each trace linked to a case shows the consecutive execution of events in a healthcare process. The predictive model is based on a long short-term memory (LSTM) neural network that achieves high accuracy in the training and testing stages. In addition, a framework to implement the LSTM neural network is proposed, comprising stages from the preprocessing of the raw data to selecting the best LSTM model. The effectiveness of the prediction method is evaluated through four real-life event logs that contain historical information on the execution of the processes of patient transfer orders between hospitals, sepsis care cases, billing of medical services, and patient care management. In the test stage, the LSTM model reached values of 0.98, 0.91, 0.85, and 0.81 in the accuracy metric, and in the evaluation of the prediction of the next event using the 10-fold cross-validation technique, values of 0.94, 0.88, 0.84, and 0.81 were obtained for the four previously mentioned event logs. In addition, the performance of the LSTM prediction model was evaluated with the precision, recall, F1-score, and area under the receiver operating characteristic (ROC) curve (AUC) metrics, obtaining high scores very close to 1. The experimental results suggest that the proposed method achieves acceptable measures in predicting the next event regardless of whether an input event or a set of input events is used.
Progressive-Augmented-Based DeepFill for High-Resolution Image Inpainting
Authors: Cui Muzi , Jiang Hao , Li Chaozhuo
Published: September 2023

Image inpainting aims to synthesize missing regions in images that are coherent with the existing visual content. Generative adversarial networks have made significant strides in the development of image inpainting. However, existing approaches heavily rely on the surrounding pixels while ignoring that the boundaries might be uninformative or noisy, leading to blurred images. As complementary, global visual features from the remote image contexts depict the overall structure and texture of the vanilla images, contributing to generating pixels that blend seamlessly with the existing visual elements. In this paper, we propose a novel model, PA-DeepFill, to repair high-resolution images. The generator network follows a novel progressive learning paradigm, starting with low-resolution images and gradually improving the resolutions by stacking more layers. A novel attention-based module, the gathered attention block, is further integrated into the generator to learn the importance of different distant visual components adaptively. In addition, we have designed a local discriminator that is more suitable for image inpainting tasks, multi-task guided mask-level local discriminator based PatchGAN, which can guide the model to distinguish between regions from the original image and regions completed by the model at a finer granularity. This local discriminator can capture more detailed local information, thereby enhancing the model’s discriminative ability and resulting in more realistic and natural inpainted images. Our proposal is extensively evaluated over popular datasets, and the experimental results demonstrate the superiority of our proposal.
Probe-Sonicated Synthesis of CuO–ZnO Hybrid Nanocomposite for Photocatalytic and Supercapacitor Applications
Authors: BaQais Amal , Alam Mir Waqas , Farhan Mohd , Muteeb Ghazala , Allag Nassiba , Mushtaq Shehla
Published: September 2023

An ultrasound-assisted probe sonication route effectively prepared pure CuO and two-dimensional CuO-ZnO nanocomposites (NCs) for different ratios of CuO and ZnO, and the experimental and theoretical methods investigated the structural, photocatalytic, and electrochemical properties. The XRD (X-ray diffraction) patterns revealed a crystallite size (D) range of 25 to 31 nm for pure CuO and CuO-ZnO NCs. According to calculations, the sample’s optical energy bandgap value (Eg) for the NCs is between 1.72 and 2.15 eV. Under UV light irradiation, the photocatalytic discoloration of pure CuO and CuO-ZnO NCs on fast blue (FB) dye was assessed. Under the influence of UV light, the CuO with 10% ZnO composite degrades 83.4% of the dye, which is greater than pure CuO and other NCs. The electrochemical properties of the prepared NCs materials have been studied using cyclic voltammetry and electrochemical impedance spectroscopy (EIS). The specific capacitance values were found to be 248 Fg−1, 301 Fg−1, 352 Fg−1, and 277 Fg−1 for CuO, CuO + 5% ZnO, CuO + 10% ZnO, and CuO + 15% ZnO, respectively, at 1 A/g current density. Galvanostatic charge–discharge tests for these designed NCs show excellent capacitance performance in supercapacitors applications. These innovative results could be considered for expanding novel resources to scale for dual applications in photocatalysis and supercapacitors.
Molecular Characterization of Serratia marcescens Strain Isolated from Yellow Mealworms, Tenebrio molitor, in The Netherlands
Authors: Bello Gonzalez Teresita d. J. , van Gelderen Betty , Harders Frank , Vloet Rianka , Voorbergen-Laarman Michal , de Ruiter Bart , Haenen Olga L. M.
Published: September 2023

Insect culture has developed rapidly worldwide; it faces important security and safety control issues, including animal infections and disease development. In the Netherlands, in 2021, a ~30% mortality of mealworms, Tenebrio molitor, occurred at one farm, where over-humid sites in the substrate were observed. Bacterial cultures from both the external and internal partsof fry and larger mealworms were identified by MALDI-TOF to predominantly Serratia marcescens, Staphylococcus xylosus and Staphylococus saprofyticus. Due to the important role of S. marcescens as a potential zoonotic bacterium, we performed a molecular characterization of the isolated strain. Genomic analysis showed a multidrug-resistant S. marcescens isolate carrying a tet (41), aac (6′)-Ic, and blaSST-1 chromosomal class C beta-lactamase-resistantgenes, all located on the chromosome. Additionally, several virulence genes were identified. The phylogenetic tree revealed that the S. marcescens strain from this study was similar to other S. marcescens strains from different ecological niches. Although the entomopathogenic activity was not confirmed, this case demonstrates that T. molitor can act as a reservoir and as an alternative path for exposing clinically important antibiotic-resistant bacteria that can affect animals and humans. It underlines the need to keep management factors optimal, before insects and their products enter the feed and food chain.