bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction of the Spatial and Temporal Adoption of an Energy Management System in Automated Dairy Cattle Barns in Bavaria—“CowEnergySystem”
Authors: Bader Christoph , Stumpenhausen Jörn , Bernhardt Heinz
Published: January 2024

In view of rising global demand, energy is becoming a significant cost factor in industry and society. In addition to the global players China, India, and the USA, Africa will also become a driver of the world’s primary energy demand in the future due to the rapidly growing developing countries. In addition to the armed conflicts in Ukraine and the Middle East, global energy markets are tense and volatile due to inflation and higher borrowing costs. Because of society’s desire to phase out the use of fossil fuels, the use of renewable energies is increasingly taking center stage worldwide and especially in Germany. Rural areas and agriculture, especially energy-intensive livestock farms, are particularly affected by this development and are therefore faced with additional economic challenges. Additional energy can be generated by using photovoltaic systems on the roofs of farm buildings or by utilizing the liquid manure from livestock farming in biogas plants. For these farms, such alternative sources of energy could open previously untapped potential and additional synergies for using their own inexpensive energy on the farm or supplying surplus electricity directly to the public grid as a market participant. Agriculture could thus serve as an actor in a decentralized energy supply and thus build up regional energy networks. However, intelligent electricity storage concepts and a corresponding energy management system (EMS) are essential to be able to utilize the potential for renewable energy generation at all, to coordinate both internal production processes and the varying energy demand and supply on the electricity grid. As agricultural production processes differ greatly from farm to farm and region to region, the introduction of an energy management system is strongly dependent on user acceptance. The purpose of this study is to use the web-based software tool ADOPT (CSIRO 2018) to predict the level of acceptance and the duration of the market launch of an EMS based on the region of Bavaria. Individual important influencing factors for the subsequent regional marketing concept are also identified.
From Food Waste to Functional Biopolymers: Characterization of Chitin and Chitosan Produced from Prepupae of Black Soldier Fly Reared with Different Food Waste-Based Diets
Authors: Mannucci Alessia , Panariello Luca , Abenaim Linda , Coltelli Maria Beatrice , Ranieri Annamaria , Conti Barbara , Santin Marco , Castagna Antonella
Published: January 2024

The use of food waste as a rearing substrate to grow insects is an ecofriendly and sustainable alternative to food waste disposal. In the present research, Hermetia illucens prepupae were reared with a standard diet, different food waste-based diets based on vegetables, fruits, and meat, and a mixed one, where the previous three components were present equally. The demineralization and deproteination of the prepupae allowed for the obtainment of chitin that was then deacetylated to produce chitosan. Also, the bleaching of chitosan was attempted for further purification. The yield of the different reactions was investigated, and the infrared spectra of the obtained materials were analyzed to obtain information on the quantity and acetylation degree trend of the chitin and chitosan as a function of the diet. The possibility to slightly modulate the yield and acetylation degree of both biopolymers thanks to the specific diet was enlightened. Interestingly, the standard diet resulted in the highest fraction of chitin having the highest acetylation degree, and in the highest fraction of chitosan having the lowest acetylation degree.
HPLC-MS/MS Phenolic Characterization of Olive Pomace Extracts Obtained Using an Innovative Mechanical Approach
Authors: Grigoletto Ilaria , García Salas Patricia , Valli Enrico , Bendini Alessandra , Ferioli Federico , Pasini Federica , Sánchez Villasclaras Sebastián , García-Ruiz Roberto , Gallina Toschi Tullia
Published: January 2024

Olive pomace results from the production of olive oil. Even if olive pomace represents a potential environmental problem, it contains phenolic compounds, which are widely recognized for their beneficial properties for human health. In this study, an innovative and sustainable technological approach to extract phenolic compounds from fresh olive pomace, based on food-grade solvent instead of those usually adopted, is investigated. Characterization and shelf-life evaluation of the hydroalcoholic extracts obtained from the procedure developed for different industrial purposes were also carried out. The phenolic fractions of the different samples were studied with the Folin–Ciocâlteu method to quantify that the total reducing molecules and HPLC-MS/MS analysis was used to define the profile through the identification and quantification of 42 compounds, belonging to five chemical families. Regarding shelf-life, the hydroalcoholic extract showed no significant reduction in phenolic content, for both instrumental evaluations, retaining most of the phenolic compounds present in the raw material; negative attributes were not perceived by sensory evaluation. Thus, these lab-scale results can be the starting point to develop a procedure that is suitable for a real olive mill, representing a valorization strategy in a circular economy and the perspective of new business models.
Numerical and Analytical Methods for Differential Equations and Systems
Authors: Gürbüz Burcu , Fernandez Arran
Published: January 2024

The theory and applications of differential equations have played an essential role both in the development of mathematics and in exploring new horizons in the applied sciences [...]
The Caputo Nonlocal Structural Derivative Ultraslow Diffusion Model of Language Change and the Microscopic Mechanism
Authors: Xu Wei , Liu Hui , Liang Yingjie , Zhao Shijun
Published: January 2024

Numerous studies have observed and analyzed the dynamics of language change from a diffusion perspective. As a complex and changeable system, the process of language change is characterized by a long memory that conforms to ultraslow diffusion. However, it is not perfectly suited for modeling with the traditional diffusion model. The Caputo nonlocal structural derivative is a further development of the classic Caputo fractional derivative. Its kernel function, characterized as an arbitrary function, proves highly effective in dealing with ultraslow diffusion. In this study, we utilized an extended logarithmic function to formulate a Caputo nonlocal structural derivative diffusion model for qualitatively analyzing the evolution process of language. The mean square displacement that grows logarithmically was derived through the Tauberian theorem and the Fourier–Laplace transform. Its effectiveness and credibility were verified by the appearance of already popular words on Japanese blogs. Compared to the random diffusion model, the Caputo nonlocal structural derivative diffusion model proves to be more precise in simulating the process of language change. The microscopic mechanism of ultraslow diffusion was explored using the continuous time random walk model, which involves a logarithmic function with a long tail. Both models incorporate memory effects, which can provide useful guidance for modeling diffusion behavior in other social phenomena.
The Effectiveness of Water- versus Land-Based Exercise on Specific Measures of Physical Fitness in Healthy Older Adults: An Integrative Review
Authors: Dalamitros Athanasios A. , Toupektsi Eirini , Alexiou Panagiota , Nousiou Stamatia , Clemente-Suarez Vicente Javier , Tornero-Aguilera José Francisco , Tsalis George
Published: January 2024

As the population ages, maintaining an active lifestyle becomes increasingly vital to promote overall health and well-being in older individuals. Water- and land-based exercises have emerged as popular options, each offering a distinct set of benefits tailored to the unique needs of this population group. An electronic database search, including PubMed, Scopus, MEDLINE, and Web of Science, was conducted until 15 September 2023, using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, to investigate the effects of water-based compared to land-based exercise on selected fitness parameters in older healthy individuals. The eligibility criteria included studies with at least two groups of participants aged 60 and older, with physical fitness outcome measures. A total of ten studies met the inclusion criteria and were analyzed. While both exercise modalities may offer significant benefits, this review’s findings emphasized the absence of conclusive evidence and consensus for recommending a single exercise category applicable to aquatic or land environments and providing more definite guidance to improve health-related physical fitness parameters in healthy older individuals. Finally, combining both training approaches may lead to a comprehensive array of health benefits for this age group population by also considering individual’s needs, preferences, and fitness goals.
Gynaecological Artificial Intelligence Diagnostics (GAID) GAID and Its Performance as a Tool for the Specialist Doctor
Authors: Tanos Panayiotis , Yiangou Ioannis , Prokopiou Giorgos , Kakas Antonis , Tanos Vasilios
Published: January 2024

Background: Human-centric artificial intelligence (HCAI) aims to provide support systems that can act as peer companions to an expert in a specific domain, by simulating their way of thinking and decision-making in solving real-life problems. The gynaecological artificial intelligence diagnostics (GAID) assistant is such a system. Based on artificial intelligence (AI) argumentation technology, it was developed to incorporate, as much as possible, a complete representation of the medical knowledge in gynaecology and to become a real-life tool that will practically enhance the quality of healthcare services and reduce stress for the clinician. Our study aimed to evaluate GAIDS’ efficacy and accuracy in assisting the working expert gynaecologist during day-to-day clinical practice. Methods: Knowledge-based systems utilize a knowledge base (theory) which holds evidence-based rules (“IF-THEN” statements) that are used to prove whether a conclusion (such as a disease, medication or treatment) is possible or not, given a set of input data. This approach uses argumentation frameworks, where rules act as claims that support a specific decision (arguments) and argue for its dominance over others. The result is a set of admissible arguments which support the final decision and explain its cause. Results: Based on seven different subcategories of gynaecological presentations—bleeding, endocrinology, cancer, pelvic pain, urogynaecology, sexually transmitted infections and vulva pathology in fifty patients—GAID demonstrates an average overall closeness accuracy of zero point eighty-seven. Since the system provides explanations for supporting a diagnosis against other possible diseases, this evaluation process further allowed for a learning process of modular improvement in the system of the diagnostic discrepancies between the system and the specialist. Conclusions: GAID successfully demonstrates an average accuracy of zero point eighty-seven when measuring the closeness of the system’s diagnosis to that of the senior consultant. The system further provides meaningful and helpful explanations for its diagnoses that can help clinicians to develop an increasing level of trust towards the system. It also provides a practical database, which can be used as a structured history-taking assistant and a friendly, patient record-keeper, while improving precision by providing a full list of differential diagnoses. Importantly, the design and implementation of the system facilitates its continuous development with a set methodology that allows minimal revision of the system in the face of new information. Further large-scale studies are required to evaluate GAID more thoroughly and to identify its limiting boundaries.
Understanding through the Numbers: Number Systems, Their Evolution, and Their Perception among Kula People from Alor Island, Southeastern Indonesia
Authors: Wu Shiyue , Perono Cacciafoco Francesco
Published: January 2024

This paper aims at documenting and reconstructing the linguistic processes generating and substantiating the use of number systems, numbers in general, elementary arithmetic, and the related concepts and notions among the Kula people from Alor Island, Southeastern Indonesia. The Kula is a Papuan population from the Alor–Pantar Archipelago (Timor area). The name of their language, Kula (or Kola), corresponds to the ethnonym. The language is, currently, endangered and not completely documented. At the level of linguistic features, numeral systems and the terms for numerals from Eastern Alor exhibit, to some extent, unique characteristics, if compared to other languages spoken in other sectors of the island. Therefore, the Kula numbering system is not only significant at the lexicological and lexicographic level, but also represents the essential role of cognitive strategies (e.g., the choice of the base for the numbering systems and the visual representation of counting with the aid of actual ‘objects’, like hands and fingers) in the coinage of numerical terms among the local speakers. Indeed, the development of numeral systems reflects the evolution of human language and the ability of humans to construct abstract numerical concepts. The way numerals are encoded and expressed in a language can impact the patterns according to which numerical notions are conceptualized and understood. Different numeral systems can indicate variations in cognitive processes involving notions of quantities and measurements. Therefore, the structure and characteristics of a numeral system may affect how numeral concepts are mentally represented and developed. This paper focuses on the number system of the Kula people and the lexical units used by the local speakers to indicate (and to explain) the numbers, with the related concepts, notions, and symbolism. The investigation delves into the degrees of abstraction of the Kula numeral system and tries to ascertain its origins and reconstruct it. Moreover, the article applies to the analysis a comparative approach, which takes into account several Papuan and Austronesian languages from Alor Island and Eastern Timor, with the dual aim of investigating, at a preliminary level, a possible common evolution and/or divergent naming processes in local numbering systems and their historical–linguistic and etymological origins.
Correction: Gheyoh Ndzi, E.; Holmes, A. Paternal Leave Entitlement and Workplace Culture: A Key Challenge to Paternal Mental Health. Int. J. Environ. Res. Public Health 2023, 20, 5454
Authors: Gheyoh Ndzi Ernestine , Holmes Amy
Published: January 2024

Amy Holmes was not included as an author in the original publication [...]
The Influence of Airbnb Announcements on North American Capital Markets: Insights for Stakeholders
Authors: Tavor Tchai
Published: January 2024

This research investigates the burgeoning peer-to-peer (P2P) economy, exemplified by platforms such as Airbnb, and its implications within the North American context. The study focuses on understanding the repercussions of Airbnb announcements on capital markets, concentrating specifically on the travel and tourism sector and the real estate sector. The findings unveil a discernible augmentation in index returns preceding the announcement’s publication in both sectors. However, a notable divergence manifests post-announcement: while the real estate sector sustains an upward trajectory in returns, the travel and tourism sector experiences a post-publication decline. These results underscore the strategic advantage available to investors with early access to Airbnb announcements, enabling them to capitalize on excess profits. Furthermore, the broader investor community can leverage the insights gleaned from Airbnb announcements for financial gains. A nuanced examination of regression results reveals the substantial impact of macroeconomic variables on index returns in both the travel and tourism sector and the real estate sector. These insights contribute to a more nuanced understanding of the intricate dynamics shaping these economic domains.
Relief Supply-Demand Estimation Based on Social Media in Typhoon Disasters Using Deep Learning and a Spatial Information Diffusion Model
Authors: Li Shaopan , Lin Yiping , Huang Hong
Published: January 2024

Estimating disaster relief supplies is crucial for governments coordinating and executing disaster relief operations. Rapid and accurate estimation of disaster relief supplies can assist the government to optimize the allocation of resources and better organize relief efforts. Traditional approaches for estimating disaster supplies are based on census data and regional risk assessments. However, these methods are often static and lack timely updates, which can result in significant disparities between the availability and demand of relief supplies. Social media, network maps, and other sources of big data contain a large amount of real-time disaster-related information that can promptly reflect the occurrence of a disaster and the relief requirements of the affected residents in a given region. Based on this information, this study presents a model to estimate the demand for disaster relief supplies using social media data. This study employs a deep learning approach to extract real-time disaster information from social media big data and integrates it with a spatial information diffusion model to estimate the population in need of relief in the affected regions. Additionally, this study estimates the demand for emergency materials based on the population in need of relief. These findings indicate that social media data can capture information on the demand for relief materials in disaster-affected regions. Moreover, integrating social media big data with traditional static data can effectively improve the accuracy and timeliness of estimating the demand for disaster relief supplies.
High-Voltage Electrostatic Field Hydrogel Microsphere 3D Culture System Improves Viability and Liver-like Properties of HepG2 Cells
Authors: Liu Yi , Ge Yang , Wu Yanfan , Feng Yongtong , Liu Han , Cao Wei , Xie Jinsong , Zhang Jingzhong
Published: January 2024

Three-dimensional (3D) hepatocyte models have become a research hotspot for evaluating drug metabolism and hepatotoxicity. Compared to two-dimensional (2D) cultures, 3D cultures are better at mimicking the morphology and microenvironment of hepatocytes in vivo. However, commonly used 3D culture techniques are not suitable for high-throughput drug screening (HTS) due to their high cost, complex handling, and inability to simulate cell–extracellular matrix (ECM) interactions. This article describes a method for rapid and reproducible 3D cell cultures with ECM–cell interactions based on 3D culture instrumentation to provide more efficient HTS. We developed a microsphere preparation based on a high-voltage electrostatic (HVE) field and used sodium alginate- and collagen-based hydrogels as scaffolds for 3D cultures of HepG2 cells. The microsphere-generating device enables the rapid and reproducible preparation of bioactive hydrogel microspheres. This 3D culture system exhibited better cell viability, heterogeneity, and drug-metabolizing activity than 2D and other 3D culture models, and the long-term culture characteristics of this system make it suitable for predicting long-term liver toxicity. This system improves the overall applicability of HepG2 spheroids in safety assessment studies, and this simple and controllable high-throughput-compatible method shows potential for use in drug toxicity screening assays and mechanistic studies.
Thymosin β4 Regulates the Differentiation of Thymocytes by Controlling the Cytoskeletal Rearrangement and Mitochondrial Transfer of Thymus Epithelial Cells
Authors: Ying Yuyuan , Tao Nana , Zhang Fengjie , Wen Xunuo , Zhou Meiru , Gao Jianli
Published: January 2024

The thymus is one of the most crucial immunological organs, undergoing visible age-related shrinkage. Thymic epithelial cells (TECs) play a vital role in maintaining the normal function of the thymus, and their degeneration is the primary cause of age-induced thymic devolution. Thymosin β4 (Tβ4) serves as a significant important G-actin sequestering peptide. The objective of this study was to explore whether Tβ4 influences thymocyte differentiation by regulating the cytoskeletal rearrangement and mitochondrial transfer of TECs. A combination of H&E staining, immunofluorescence, transmission electron microscopy, RT-qPCR, flow cytometry, cytoskeletal immunolabeling, and mitochondrial immunolabeling were employed to observe the effects of Tβ4 on TECs’ skeleton rearrangement, mitochondrial transfer, and thymocyte differentiation. The study revealed that the Tβ4 primarily regulates the formation of microfilaments and the mitochondrial transfer of TECs, along with the formation and maturation of double-negative cells (CD4−CD8−) and CD4 single-positive cells (CD3+TCRβ+CD4+CD8−) thymocytes. This study suggests that Tβ4 plays a crucial role in thymocyte differentiation by influencing the cytoskeletal rearrangement and mitochondrial transfer of TECs. These effects may be associated with Tβ4’s impact on the aggregation of F-actin. This finding opens up new avenues for research in the field of immune aging.
Special Issue: “Drug Repurposing for Cancer Therapies”
Authors: Xavier Cristina P. R. , Palmeira Andreia
Published: January 2024

Cancer is one of the primary global causes of death, thus addressing cancer therapy remains a significant challenge, especially in cases where cancers exhibit resistance to treatment [...]
Organoids: An Emerging Precision Medicine Model for Prostate Cancer Research
Authors: Waseem Mohammad , Wang Bi-Dar
Published: January 2024

Prostate cancer (PCa) has been known as the most prevalent cancer disease and the second leading cause of cancer mortality in men almost all over the globe. There is an urgent need for establishment of PCa models that can recapitulate the progress of genomic landscapes and molecular alterations during development and progression of this disease. Notably, several organoid models have been developed for assessing the complex interaction between PCa and its surrounding microenvironment. In recent years, PCa organoids have been emerged as powerful in vitro 3D model systems that recapitulate the molecular features (such as genomic/epigenomic changes and tumor microenvironment) of PCa metastatic tumors. In addition, application of organoid technology in mechanistic studies (i.e., for understanding cellular/subcellular and molecular alterations) and translational medicine has been recognized as a promising approach for facilitating the development of potential biomarkers and novel therapeutic strategies. In this review, we summarize the application of PCa organoids in the high-throughput screening and establishment of relevant xenografts for developing novel therapeutics for metastatic, castration resistant, and neuroendocrine PCa. These organoid-based studies are expected to expand our knowledge from basic research to clinical applications for PCa diseases. Furthermore, we also highlight the optimization of PCa cultures and establishment of promising 3D organoid models for in vitro and in vivo investigations, ultimately facilitating mechanistic studies and development of novel clinical diagnosis/prognosis and therapies for PCa.
Microbial Therapy and Breast Cancer Management: Exploring Mechanisms, Clinical Efficacy, and Integration within the One Health Approach
Authors: Filippou Charalampos , Themistocleous Sophia C. , Marangos Giorgos , Panayiotou Yiannis , Fyrilla Maria , Kousparou Christina A. , Pana Zoi-Dorothea , Tsioutis Constantinos , Johnson Elizabeth O. , Yiallouris Andreas
Published: January 2024

This comprehensive review elucidates the profound relationship between the human microbiome and breast cancer management. Recent findings highlight the significance of microbial alterations in tissue, such as the gut and the breast, and their role in influencing the breast cancer risk, development, progression, and treatment outcomes. We delve into how the gut microbiome can modulate systemic inflammatory responses and estrogen levels, thereby impacting cancer initiation and therapeutic drug efficacy. Furthermore, we explore the unique microbial diversity within breast tissue, indicating potential imbalances brought about by cancer and highlighting specific microbes as promising therapeutic targets. Emphasizing a holistic One Health approach, this review underscores the importance of integrating insights from human, animal, and environmental health to gain a deeper understanding of the complex microbe–cancer interplay. As the field advances, the strategic manipulation of the microbiome and its metabolites presents innovative prospects for the enhancement of cancer diagnostics and therapeutics. However, rigorous clinical trials remain essential to confirm the potential of microbiota-based interventions in breast cancer management.
The Effects of DNA Methylation on Cytoplasmic Male Sterility in Sugar Beet
Authors: Weng Jiamin , Wang Hui , Cheng Dayou , Liu Tianjiao , Zeng Deyong , Dai Cuihong , Luo Chengfei
Published: January 2024

DNA methylation is widely found in higher plants and can control gene expression by regulation without changing the DNA sequence. In this study, the whole-genome methylation map of sugar beet was constructed by WGBS (whole-genome bisulfite sequencing) technology, and the results of WGBS were verified by bisulfite transformation, indicating that the results of WGBS technology were reliable. In addition, 12 differential methylation genes (DMGs) were identified, which were related to carbohydrate and energy metabolism, pollen wall development, and endogenous hormone regulation. Quantitative real-time PCR (qRT-PCR) showed that 75% of DMG expression levels showed negative feedback with methylation level, indicating that DNA methylation can affect gene expression to a certain extent. In addition, we found hypermethylation inhibited gene expression, which laid a foundation for further study on the molecular mechanism of DNA methylation at the epigenetic level in sugar beet male sterility.
The Healthy and Diseased Retina Seen through Neuron–Glia Interactions
Authors: Tempone Matheus H. , Borges-Martins Vladimir P. , César Felipe , Alexandrino-Mattos Dio Pablo , de Figueiredo Camila S. , Raony Ícaro , dos Santos Aline Araujo , Duarte-Silva Aline Teixeira , Dias Mariana Santana , Freitas Hércules Rezende , de Araújo Elisabeth G. , Ribeiro-Resende Victor Tulio , Cossenza Marcelo , P. Silva Hilda , P. de Carvalho Roberto , Ventura Ana L. M. , Calaza Karin C. , Silveira Mariana S. , Kubrusly Regina C. C. , de Melo Reis Ricardo A.
Published: January 2024

The retina is the sensory tissue responsible for the first stages of visual processing, with a conserved anatomy and functional architecture among vertebrates. To date, retinal eye diseases, such as diabetic retinopathy, age-related macular degeneration, retinitis pigmentosa, glaucoma, and others, affect nearly 170 million people worldwide, resulting in vision loss and blindness. To tackle retinal disorders, the developing retina has been explored as a versatile model to study intercellular signaling, as it presents a broad neurochemical repertoire that has been approached in the last decades in terms of signaling and diseases. Retina, dissociated and arranged as typical cultures, as mixed or neuron- and glia-enriched, and/or organized as neurospheres and/or as organoids, are valuable to understand both neuronal and glial compartments, which have contributed to revealing roles and mechanisms between transmitter systems as well as antioxidants, trophic factors, and extracellular matrix proteins. Overall, contributions in understanding neurogenesis, tissue development, differentiation, connectivity, plasticity, and cell death are widely described. A complete access to the genome of several vertebrates, as well as the recent transcriptome at the single cell level at different stages of development, also anticipates future advances in providing cues to target blinding diseases or retinal dysfunctions.
Long-Term Exposure of Cultured Astrocytes to High Glucose Impact on Their LPS-Induced Activation
Authors: Abdyeva Ayna , Kurtova Ekaterina , Savinkova Irina , Galkov Maksim , Gorbacheva Liubov
Published: January 2024

Diabetes mellitus is associated with various complications, mainly caused by the chronic exposure of the cells to high glucose (HG) concentrations. The effects of long-term HG exposure in vitro accompanied by lipopolysaccharide (LPS) application on astrocytes are relatively unknown. We used cell medium with normal (NG, 5.5 mM) or high glucose (HG, 25 mM) for rat astrocyte cultures and measured the release of NO, IL-6, β-hexosaminidase and cell survival in response to LPS. We first demonstrated that HG long-term incubation of astrocytes increased the release of β-hexosaminidase without decreasing MTT-detected cell survival, suggesting that there is no cell membrane damage or astrocyte death but could be lysosome exocytosis. Different from what was observed for NG, all LPS concentrations tested at HG resulted in an increase in IL-6, and this was detected for both 6 h and 48 h treatments. Interestingly, β-hexosaminidase level increased after 48 h of LPS and only at HG. The NO release from astrocytes also increased with LPS application at HG but was less significant. These data endorsed the original hypothesis that long-term hyperglycemia increases proinflammatory activation of astrocytes, and β-hexosaminidase could be a specific marker of excessive activation of astrocytes associated with exocytosis.
Maternal Diet High in Linoleic Acid Alters Offspring Lipids and Hepatic Regulators of Lipid Metabolism in an Adolescent Rat Model
Authors: Shrestha Nirajan , Sleep Simone L. , Holland Olivia J. , Vidimce Josif , Bulmer Andrew C. , Cuffe James S. M. , Perkins Anthony V. , McAinch Andrew J. , Hryciw Deanne H.
Published: January 2024

Linoleic acid (LA), an n-6 polyunsaturated fatty acid (PUFA), is essential for fetal growth and development. A maternal high LA (HLA) diet alters cardiovascular development in adolescent rats and hepatic function in adult rats in a sex-specific manner. We investigated the effects of an HLA diet on adolescent offspring hepatic lipids and hepatic lipid metabolism gene expression, and the ability of the postnatal diet to alter these effects. Female Wistar Kyoto rats were fed low LA (LLA; 1.44% energy from LA) or high LA (HLA; 6.21% energy from LA) diets during pregnancy and gestation/lactation. Offspring, weaned at postnatal day (PN) 25, were fed LLA or HLA and euthanised at PN40 (n = 6–8). Maternal HLA increased circulating uric acid, decreased hepatic cholesterol and increased hepatic Pparg in males, whereas only hepatic Srebf1 and Hmgcr increased in females. Postnatal (post-weaning) HLA decreased liver weight (% body weight) and increased hepatic Hmgcr in males, and decreased hepatic triglycerides in females. Maternal and postnatal HLA had an interaction effect on Lpl, Cpt1a and Pparg in females. These findings suggest that an HLA diet both during and after pregnancy should be avoided to improve offspring disease risk.
Identification of Gene Responsible for Conferring Resistance against Race KN2 of Podosphaera xanthii in Melon
Authors: Kheng Sopheak , Choe San-Ha , Sahu Nihar , Park Jong-In , Kim Hoy-Taek
Published: January 2024

Powdery mildew caused by Podosphaera xanthii is a serious fungal disease which causes severe damage to melon production. Unlike with chemical fungicides, managing this disease with resistance varieties is cost effective and ecofriendly. But, the occurrence of new races and a breakdown of the existing resistance genes poses a great threat. Therefore, this study aimed to identify the resistance locus responsible for conferring resistance against P. xanthii race KN2 in melon line IML107. A bi-parental F2 population was used in this study to uncover the resistance against race KN2. Genetic analysis revealed the resistance to be monogenic and controlled by a single dominant gene in IML107. Initial marker analysis revealed the position of the gene to be located on chromosome 2 where many of the resistance gene against P. xanthii have been previously reported. Availability of the whole genome of melon and its R gene analysis facilitated the identification of a F-box type Leucine Rich Repeats (LRR) to be accountable for the resistance against race KN2 in IML107. The molecular marker developed in this study can be used for marker assisted breeding programs.
Improved Solubility and Stability of a Thermostable Carbonic Anhydrase via Fusion with Marine-Derived Intrinsically Disordered Solubility Enhancers
Authors: Jo Byung Hoon
Published: January 2024

Carbonic anhydrase (CA), an enzyme catalyzing the reversible hydration reaction of carbon dioxide (CO2), is considered a promising biocatalyst for CO2 reduction. The α-CA of Thermovibrio ammonificans (taCA) has emerged as a compelling candidate due to its high thermostability, a critical factor for industrial applications. However, the low-level expression and poor in vitro solubility have hampered further utilization of taCA. Recently, these limitations have been addressed through the fusion of the NEXT tag, a marine-derived, intrinsically disordered small peptide that enhances protein expression and solubility. In this study, the solubility and stability of NEXT-taCA were further investigated. When the linker length between the NEXT tag and the taCA was shortened, the expression level decreased without compromising solubility-enhancing performance. A comparison between the NEXT tag and the NT11 tag demonstrated the NEXT tag’s superiority in improving both the expression and solubility of taCA. While the thermostability of taCA was lower than that of the extensively engineered DvCA10, the NEXT-tagged taCA exhibited a 30% improvement in long-term thermostability compared to the untagged taCA, suggesting that enhanced solubility can contribute to enzyme thermostability. Furthermore, the bioprospecting of two intrinsically disordered peptides (Hcr and Hku tags) as novel solubility-enhancing fusion tags was explored, demonstrating their performance in improving the expression and solubility of taCA. These efforts will advance the practical application of taCA and provide tools and insights for enzyme biochemistry and bioengineering.
Exercise-Intervened Endothelial Progenitor Cell Exosomes Protect N2a Cells by Improving Mitochondrial Function
Authors: Chen Shuzhen , Sigdel Smara , Sawant Harshal , Bihl Ji , Wang Jinju
Published: January 2024

We have recently demonstrated that exosomal communication between endothelial progenitor cells (EPCs) and brain endothelial cells is compromised in hypertensive conditions, which might contribute to the poor outcomes of stroke subjects with hypertension. The present study investigated whether exercise intervention can regulate EPC–exosome (EPC-EX) functions in hypertensive conditions. Bone marrow EPCs from sedentary and exercised hypertensive transgenic mice were used for generating EPC-EXs, denoted as R-EPC-EXs and R-EPC-EXET. The exosomal microRNA profile was analyzed, and EX functions were determined in a co-culture system with N2a cells challenged by angiotensin II (Ang II) plus hypoxia. EX-uptake efficiency, cellular survival ability, reactive oxygen species (ROS) production, mitochondrial membrane potential, and the expressions of cytochrome c and superoxide-generating enzyme (Nox4) were assessed. We found that (1) exercise intervention improves the uptake efficiency of EPC-EXs by N2a cells. (2) exercise intervention restores miR-27a levels in R-EPC-EXs. (3) R-EPC-EXET improved the survival ability and reduced ROS overproduction in N2a cells challenged with Ang II and hypoxia. (4) R-EPC-EXET improved the mitochondrial membrane potential and decreased cytochrome c and Nox4 levels in Ang II plus hypoxia-injured N2a cells. All these effects were significantly reduced by miR-27a inhibitor. Together, these data have demonstrated that exercise-intervened EPC-EXs improved the mitochondrial function of N2a cells in hypertensive conditions, which might be ascribed to their carried miR-27a.
Disruption of the Arabidopsis Acyl-Activating Enzyme 3 Impairs Seed Coat Mucilage Accumulation and Seed Germination
Authors: Cheng Ninghui , Nakata Paul A.
Published: January 2024

The Acyl-activating enzyme (AAE) 3 gene encodes an oxalyl-CoA synthetase that catalyzes the conversion of oxalate to oxalyl-CoA as the first step in the CoA-dependent pathway of oxalate catabolism. Although the role of this enzyme in oxalate catabolism has been established, its biological roles in plant growth and development are less understood. As a step toward gaining a better understanding of these biological roles, we report here a characterization of the Arabidopsis thaliana aae3 (Ataae3) seed mucilage phenotype. Ruthidium red (RR) staining of Ataae3 and wild type (WT) seeds suggested that the observed reduction in Ataae3 germination may be attributable, at least in part, to a decrease in seed mucilage accumulation. Quantitative RT-PCR analysis revealed that the expression of selected mucilage regulatory transcription factors, as well as of biosynthetic and extrusion genes, was significantly down-regulated in the Ataae3 seeds. Mucilage accumulation in seeds from an engineered oxalate-accumulating Arabidopsis and Atoxc mutant, blocked in the second step of the CoA-dependent pathway of oxalate catabolism, were found to be similar to WT. These findings suggest that elevated tissue oxalate concentrations and loss of the oxalate catabolism pathway downstream of AAE3 were not responsible for the reduced Ataae3 seed germination and mucilage phenotypes. Overall, our findings unveil the presence of regulatory interplay between AAE3 and transcriptional control of mucilage gene expression.
Correlation between the Closure Time of Patent Ductus Arteriosus in Preterm Infants and Long-Term Neurodevelopmental Outcome
Authors: Kikuchi Natsumi , Goto Taichiro , Katsumata Nobuyuki , Murakami Yasushi , Shinohara Tamao , Maebayashi Yuki , Sakakibara Aiko , Saito Chisato , Hasebe Yohei , Hoshiai Minako , Nemoto Atsushi , Naito Atsushi
Published: January 2024

In patent ductus arteriosus (PDA) in preterm infants, the relationship between treatment timing and long-term developmental prognosis remains unclear. The purpose of this study was to clarify the relationship between the age in days when ductus arteriosus closure occurred and long-term development. Preterm infants with a birth weight of less than 1500 g who were admitted to our NICU over a period of 9 years (2011–2019) and were diagnosed with PDA were included. A new version of the K-type developmental test for corrected ages of 1.5 and 3 years was used as an index of development. The relationship between the duration of PDA and the developmental index was evaluated using Pearson’s correlation coefficient, and multiple regression analysis was performed. Development quotient (DQ) at the ages of 1.5 and 3 years showed a correlation with the PDA closure date and the standard deviation (SD) value of the term birth weight. Multiple regression analysis showed a positive correlation of the DQ at 1.5 and 3 years with the SD value of the term birth weight and a negative correlation with the PDA closure date. In addition, a stronger correlation was found in the “posture/motor” sub-item at 3 years. On the other hand, the analysis including preterm infants without PDA showed that preterm infants with PDA closure on the 6th day or later after birth had a significantly lower 3-year-old DQ than preterm infants with a PDA exposure within 5 days. In conclusion, it is suggested that the decrease in cerebral blood flow due to PDA in preterm infants has an adverse effect on long-term neurodevelopment. Appropriate interventions, including surgical treatment for PDA in preterm infants without delay, ideally within 5 days of birth, may be effective in improving the developmental prognosis.
Dietary Salt Restriction and Adherence to the Mediterranean Diet: A Single Way to Reduce Cardiovascular Risk?
Authors: D’Elia Lanfranco , Strazzullo Pasquale
Published: January 2024

The dietary restriction of salt intake and the adhesion to Mediterranean dietary patterns are among the most recommended lifestyle modifications for the prevention of cardiovascular diseases. A large amount of evidence supports these recommendations; indeed, several studies show that a higher adherence to Mediterranean dietary patterns is associated with a reduced risk of cardiovascular disease. Likewise, findings from observational and clinical studies suggest a causal role of excess salt intake in blood pressure increase, cardiovascular organ damage, and the incidence of cardiovascular diseases. In this context, it is also conceivable that the beneficial effects of these two dietary patterns overlap because Mediterranean dietary patterns are typically characterized by a large consumption of plant-based foods with low sodium content. However, there is little data on this issue, and heterogeneous results are available on the relationship between adherence to salt restriction and to Mediterranean dietary patterns. Thus, this short review focuses on the epidemiological and clinical evidence of the relationship between the adherence to Mediterranean dietary patterns and dietary salt restriction in the context of cardiovascular risk.
Autoimmune Polyendocrine Syndromes in Adult Italian Celiac Disease Patients
Authors: Pallotta Dante Pio , Granito Alessandro , Raiteri Alberto , Boe Maria , Pratelli Agnese , Giamperoli Alice , Monaco Giovanni , Faggiano Chiara , Tovoli Francesco
Published: January 2024

Celiac disease (CD) is frequently associated with other autoimmune disorders. Different studies have explored the association between CD and single autoimmune endocrine disease (AED), especially autoimmune thyroiditis (AIT) and type-1 diabetes mellitus (T1DM). Data about CD as a component of autoimmune polyendocrine syndrome (APS) are scant. We analyzed a large dataset including prospectively collected data from 920 consecutive adult CD patients diagnosed in a third-level Italian institution in the 2013–2023 period, The prevalence of isolated autoimmune endocrine diseases and APS were collected. A total of 262 (28.5%) CD patients had at least one associated AED, with AIT (n = 223, 24.2%) and T1DM (n = 27, 2.9%) being the most frequent conditions. In most cases (n = 173, 66%), AEDs were diagnosed after CD. Thirteen patients (1.4%) had at least two of the requested three endocrinopathies, satisfying the diagnosis of APS. APS is a rare but not exceptional occurrence among Italian CD patients, underscoring the intricate and multifaceted nature of autoimmune disorders. Periodic evaluations of thyroid function and glycaemia should be recommended after the diagnosis of CD together with testing for autoantibodies that may be helpful in assessing disease risk before disease onset. Likewise, implementation of a systematic screening for CD amongst T1DM and other autoimmune endocrine diseases are paramount.
Feasibility and Safety of Adopting a New Approach in Delivering a 450 nm Blue Laser with a Flattop Beam Profile in Vital Tooth Whitening. A Clinical Case Series with an 8-Month Follow-Up
Authors: Hanna Reem , Miron Ioana Cristina , Benedicenti Stefano
Published: January 2024

A prospective observational case series included six patients who presented with discoloured upper and lower teeth extending from the right second premolar to the left second premolar. The photoactivation dosimetry and treatment protocol were as follows: λ 450 nm, 1 W, CW; flattop beam profile; 1 cm2; 15 J/spot; 10 irradiated spots; an irradiation time of 15 s/spot; three whitening cycles in a single session. Blanc One ULTRA+ was the bleaching agent. A visual analogue scale (VAS) was utilised to evaluate the pain intensity and dental hypersensitivity during treatment immediately after complete treatment (T1), 24 h (T2), and 8 h (T3) postoperatively, and at an 8-month follow-up timepoint (T4), whereas the dental colour shade change was assessed using the VITA colour shade guide pre-treatment (T0), T1, and T4. The Gingival index and modified Wong Baker faces scale were utilised to evaluate gingival inflammation and patients’ treatment satisfaction, respectively. Our findings revealed a reduction in the dental colour shade of the six cases between 2 and 10- fold (average of 3.5-fold) at T1 and maintained at T4, indicating significant improvement in the colour shade change with optimal outcomes. The percentage of this improvement for all the patients was ranged between 16.6% and 33.3%. At all timepoints, a “0” score was provided for pain intensity, dental hypersensitivity, and gingival inflammation. Our study demonstrates the feasibility and safety of a λ 450 nm laser delivered with a flattop handpiece to achieve optimal whitening outcomes without adverse effects. This offers a useful guide for dental clinicians for vital in-office tooth whitening. Extensive clinical studies with large data are warranted to validate our study protocol.
Tone Decay Reconsidered: Preliminary Results of a Prospective Study in Hearing-Aid Users with Moderate to Severe Hearing Loss
Authors: Schmidt Florian Herrmann , Hocke Thomas , Zhang Lichun , Großmann Wilma , Mlynski Robert
Published: January 2024

Among hearing aid (HA) users, there is a considerable variability in word recognition scores (WRSs). This variability is most pronounced among individuals with moderately severe to severe hearing loss. The variability cannot be adequately explained by factors such as pure-tone audiogram, audiogram type or age. This prospective study was designed to investigate the relationship between tone decay (TD) and WRS in a group of HA users with corresponding pure-tone hearing loss. The study population included 22 patients with hearing loss between 50 and 80 dB HL. Aided WRS, unaided WRS and TD were assessed for both ears. TD was found to be frequency-dependent. TD and WRS were correlated, with up to R = −0.66. The TD test was revealed to be a feasible method for explaining variability in WRS among HA users with hearing loss below 80 dB. This may contribute to improved differential diagnostics. The TD test may thus offer a better understanding of the limitations of HA use in the context of cochlear implant candidacy assessment for HA users.