bluuebunny/crossref_metadata_2025 huggingface dataset:
Formation and Properties of Oxide Coatings with Immobilized Zeolites Obtained by Plasma Electrolytic Oxidation of Aluminum
Authors: Mojsilović Kristina , Lačnjevac Uroš , Stojanović Srna , Damjanović-Vasilić Ljiljana , Stojadinović Stevan , Vasilić Rastko
Published: August 2021

In this paper, we employed plasma electrolytic oxidation (PEO) of aluminum in a water solution of sodium tungstate (Na2WO4∙2H2O) with the addition of the pure and Ce-loaded zeolites clinoptilolite and 13 X for the preparation of oxide coatings. The obtained coatings were characterized with respect to their morphologies and chemical and phase compositions using scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy, atomic force microscopy, and X-ray diffraction. The prepared coatings contained γ-alumina, WO3, and metallic tungsten. The surface morphologies of the obtained coatings strongly depended on the PEO processing time; the roughness of all coatings increased with PEO time, while porosity decreased with PEO processing time as a result of microdischarge coalescence and growth. All coatings contained elements originating from the substrate and from the electrolytes. Coatings containing zeolites with Ce showed higher photoactivity than those with immobilized pure zeolites. The highest photocatalytic activity levels were observed for coatings containing immobilized Ce-exchanged clinoptilolite processed for 10 min. It was observed that both clinoptilolite and 13X zeolites improved the features of the PEO coatings in a similar manner, making natural and abundant clinoptilolite an excellent candidate for various applications.
Inside the Insulin Secretory Granule
Authors: Germanos Mark , Gao Andy , Taper Matthew , Yau Belinda , Kebede Melkam A.
Published: August 2021

The pancreatic β-cell is purpose-built for the production and secretion of insulin, the only hormone that can remove glucose from the bloodstream. Insulin is kept inside miniature membrane-bound storage compartments known as secretory granules (SGs), and these specialized organelles can readily fuse with the plasma membrane upon cellular stimulation to release insulin. Insulin is synthesized in the endoplasmic reticulum (ER) as a biologically inactive precursor, proinsulin, along with several other proteins that will also become members of the insulin SG. Their coordinated synthesis enables synchronized transit through the ER and Golgi apparatus for congregation at the trans-Golgi network, the initiating site of SG biogenesis. Here, proinsulin and its constituents enter the SG where conditions are optimized for proinsulin processing into insulin and subsequent insulin storage. A healthy β-cell is continually generating SGs to supply insulin in vast excess to what is secreted. Conversely, in type 2 diabetes (T2D), the inability of failing β-cells to secrete may be due to the limited biosynthesis of new insulin. Factors that drive the formation and maturation of SGs and thus the production of insulin are therefore critical for systemic glucose control. Here, we detail the formative hours of the insulin SG from the luminal perspective. We do this by mapping the journey of individual members of the SG as they contribute to its genesis.
Metabolomic Recovery as a Result of Ischemic Preconditioning Was More Pronounced in Hippocampus than in Cortex That Appeared More Sensitive to Metabolomic Blood Components
Authors: Baranovicova Eva , Kalenska Dagmar , Grendar Marian , Lehotsky Jan
Published: August 2021

The study of an organism’s response to ischemia at different levels is essential to understand the mechanism of the injury as well as protection. We used the occlusion of four vessels as an animal model of global cerebral ischemia to investigate metabolic alterations in cerebral cortex, hippocampus, blood plasma, as well as in a remote organ, the heart, in rats undergoing 24 h postischemic reperfusion. By inducing sublethal ischemic stimuli, we focused on endogenous phenomena known as ischemic tolerance that is currently the best known and most effective way of protecting against ischemic injury. NMR spectroscopy was used to analyze relative metabolite levels in homogenates from rats’ cerebral cortex, hippocampus, and heart together with deproteinized blood plasma. In individual animals subjected to global cerebral ischemia, relative concentrations of the essential amino acids isoleucine, valine, phenylalanine, and tyrosine in cerebral cortex correlated with those in blood plasma (p < 0.05, or boundary significant p < 0.09). This did not apply for the hippocampus, suggesting a closer relation between ischemic cortex and metabolomic blood components. Hippocampal non-participation on correlation with blood components may emphasize the observed partial or full normalization the post-ischemically altered levels of a number of metabolites in the preconditioned animals. Remarkably, that was observed for cortex to a lesser extent. As a response to the global cerebral ischemia in heart tissue, we observed decreased glutamate and increased 3-hydroxybutyrate. Ischemically induced semi-ketotic state and other changes found in blood plasma partially normalized when ischemic preconditioning was introduced. Some metabolomic changes were so strong that even individual metabolites were able to differentiate between ischemic, ischemically preconditioned, and control brain tissues.
Static and Dynamic Analysis of Electrostatically Actuated MEMS Shallow Arches for Various Air-Gap Configurations
Authors: Ouakad Hassen M. , Alcheikh Nouha , Younis Mohammad I.
Published: August 2021

In this research, we investigate the structural behavior, including the snap-through and pull-in instabilities, of in-plane microelectromechanical COSINE-shaped and electrically actuated clamped-clamped micro-beams resonators. The work examines various electrostatic actuation patterns including uniform and non-uniform parallel-plates airgap arrangements, which offer options to actuate the arches in the opposite and same direction of their curvature. The nonlinear equation of motion of a shallow arch is discretized into a reduced-order model based on the Galerkin’s expansion method, which is then numerically solved. Static responses are examined for various DC electrostatic loads starting from small values to large values near pull-in and snap-through instability ranges, if any. The eigenvalue problem of the micro-beam is solved revealing the variations of the first four natural frequencies as varying the DC load. Various simulations are carried out for several case studies of shallow arches of various geometrical parameters and airgap arrangements, which demonstrate rich and diverse static and dynamic behaviors. Results show few cases with multi-states and hysteresis behaviors where some with only the pull-in instability and others with both snap-through buckling and pull-in instabilities. It is found that the micro-arches behaviors are very sensitive to the electrode’s configuration. The studied configurations reveal different possibilities to control the pull-in and snap-through instabilities, which can be used for improving arches static stroke range as actuators and for realizing wide-range tunable micro-resonators.
In Vitro Antimicrobial Susceptibility Profiles of Gram-Positive Anaerobic Cocci Responsible for Human Invasive Infections
Authors: Guérin François , Dejoies Loren , Degand Nicolas , Guet-Revillet Hélène , Janvier Frédéric , Corvec Stéphane , Barraud Olivier , Guillard Thomas , Walewski Violaine , Gallois Emmanuelle , Cattoir Vincent ,   on behalf of the GMC Study Group
Published: August 2021

The aim of this multicentre study was to determine the in vitro susceptibility to anti-anaerobic antibiotics of Gram-positive anaerobic cocci (GPAC) isolates responsible for invasive infections in humans. A total of 133 GPAC isolates were collected in nine French hospitals from 2016 to 2020. All strains were identified to the species level (MALDI-TOF mass spectrometry, 16S rRNA sequencing). Minimum inhibitory concentrations (MICs) of amoxicillin, piperacillin, cefotaxime, imipenem, clindamycin, vancomycin, linezolid, moxifloxacin, rifampicin, and metronidazole were determined by the reference agar dilution method. Main erm-like genes were detected by PCR. The 133 GPAC isolates were identified as follows: 10 Anaerococcus spp., 49 Finegoldia magna, 33 Parvimonas micra, 30 Peptoniphilus spp., and 11 Peptostreptococcus anaerobius. All isolates were susceptible to imipenem, vancomycin (except 3 P. micra), linezolid and metronidazole. All isolates were susceptible to amoxicillin and piperacillin, except for P. anaerobius (54% and 45% susceptibility only, respectively). MICs of cefotaxime widely varied while activity of rifampicin, and moxifloxacin was also variable. Concerning clindamycin, 31 were categorized as resistant (22 erm(A) subclass erm(TR), 7 erm(B), 1 both genes and 1 negative for tested erm genes) with MICs from 8 to >32 mg/L. Although GPACs are usually susceptible to drugs commonly used for the treatment of anaerobic infections, antimicrobial susceptibility should be evaluated in vitro.
Evaluation of Biocompatibility and Antagonistic Properties of Microorganisms Isolated from Natural Sources for Obtaining Biofertilizers Using Microalgae Hydrolysate
Authors: Babich Olga , Sukhikh Stanislav , Dyshlyuk Lyubov , Shishko Olga , Milentyeva Irina , Prosekov Alexander , Pavsky Valery , Ivanova Svetlana , Dolganyuk Vyacheslav
Published: August 2021

Determination of the biocompatibility of microorganisms isolated from natural sources (Kemerovo Oblast—Kuzbass) resulted in the creation of three microbial consortia based on the isolated strains: consortium I (Bacillus pumilus, Pediococcus damnosus, and Pediococcus pentosaceus), consortium II (Acetobacter aceti, Pseudomonas chlororaphis, and Streptomyces parvus), and consortium III (Amycolatopsis sacchari, Bacillus stearothermophilus; Streptomyces thermocarboxydus; and Streptomyces thermospinisporus). The nutrient media composition for the cultivation of each of the three studied microbial consortia, providing the maximum increase in biomass, was selected: consortium I, nutrient medium 11; consortium II, nutrient medium 13; for consortium III, nutrient medium 16. Consortia I and II microorganisms were cultured at 5–25 °C, and consortium III at 50–70 °C. Six types of psychrophilic microorganisms (P. pentosaceus, P. chlororaphis, P. damnosus, B. pumilus, A. aceti, and S. parvus) and four types of thermophilic microorganisms (B. stearothermophilus, S. thermocarboxydus, S. thermospinisporus, and A. sacchari) were found to have high antagonistic activity against the tested pathogenic strains (A. faecalis, B. cinerea, E. carotovora, P. aeruginosa, P. fluorescens, R. stolonifera, X. vesicatoria. pv. Vesicatoria, and E. aphidicola). The introduction of microalgae hydrolyzate increased the concentration of microorganisms by 5.23 times in consortium I, by 4.66 times in consortium II, by 6.6 times in consortium III. These data confirmed the efficiency (feasibility) of introducing microalgae hydrolyzate into the biofertilizer composition.
Distribution of SARS-CoV-2 Lineages in the Czech Republic, Analysis of Data from the First Year of the Pandemic
Authors: Klempt Petr , Brzoň Ondřej , Kašný Martin , Kvapilová Kateřina , Hubáček Petr , Briksi Aleš , Bezdíček Matěj , Koudeláková Vladimira , Lengerová Martina , Hajdúch Marian , Dřevínek Pavel , Pospíšilová Šárka , Kriegová Eva , Macek Milan , Kvapil Petr
Published: August 2021

In the Czech Republic, the current pandemic led to over 1.67 million SARS-CoV-2- positive cases since the recording of the first case on 1 March 2020. SARS-CoV-2 genome analysis is an important tool for effective real-time quantitative PCR (RT-qPCR) diagnostics, epidemiology monitoring, as well as vaccination strategy. To date, there is no comprehensive report on the distribution of SARS-CoV-2 genome variants in either the Czech Republic, including Central and Eastern Europe in general, during the first year of pandemic. In this study, we have analysed a representative cohort of SARS-CoV-2 genomes from 229 nasopharyngeal swabs of COVID-19 positive patients collected between March 2020 and February 2021 using validated reference-based sequencing workflow. We document the changing frequency of dominant variants of SARS-CoV-2 (from B.1 -> B.1.1.266 -> B.1.258 -> B.1.1.7) throughout the first year of the pandemic and list specific variants that could impact the diagnostic efficiency RT-qPCR assays. Moreover, our reference-based workflow provided evidence of superinfection in several samples, which may have contributed to one of the highest per capita numbers of COVID-19 cases and deaths during the first year of the pandemic in the Czech Republic.
Characterization and Structural Insights of the Reaction Products by Direct Leaching of the Noble Metals Au, Pd and Cu with N,N′-Dimethyl-piperazine-2,3-dithione/I2 Mixtures
Authors: Serpe Angela , Pilia Luca , Balestri Davide , Marchiò Luciano , Deplano Paola
Published: August 2021

In the context of new efficient and safe leaching agents for noble metals, this paper describes the capability of the Me2pipdt/I2 mixture (where Me2pipdt = N,N′-dimethyl-piperazine-2,3-dithione) in organic solutions to quantitatively dissolve Au, Pd, and Cu metal powders in mild conditions (room temperature and pressure) and short times (within 1 h in the reported conditions). A focus on the structural insights of the obtained coordination compounds is shown, namely [AuI2(Me2pipdt)]I3 (1), [Pd(Me2pipdt)2]I2 (2a) and [Cu(Me2pipdt)2]I3 (3), where the metals are found, respectively, in 3+, 2+ and 1+ oxidation states, and of [Cu(Me2pipdt)2]BF4 (4) and [Cu(Me2dazdt)2]I3 (5) (Me2dazdt = N,N′-dimethyl-perhydrodizepine-2,3-dithione) compared with 3. Au(III) and Pd(II) (d8 configuration) form square–planar complexes, whereas Cu(I) (d10) forms tetrahedral complexes. Density functional theory calculations performed on the cationic species of 1–5 help to highlight the nature of the bonding in the different complexes. Finally, the valorization of the noble metals-rich leachates is assessed. Specifically, gold metal is quantitatively recovered from the solution besides the ligands, showing the potential of these systems to promote metal recycling processes.
Analysis of Antioxidant Capacity and Antimicrobial Properties of Selected Polish Grape Vinegars Obtained by Spontaneous Fermentation
Authors: Antoniewicz Justyna , Jakubczyk Karolina , Kwiatkowski Paweł , Maciejewska-Markiewicz Dominika , Kochman Joanna , Rębacz-Maron Ewa , Janda-Milczarek Katarzyna
Published: August 2021

Nowadays, products of natural origin with health-promoting properties are increasingly more common. Research shows that fruit vinegars can be a source of compounds with antioxidant activity. Research on the total antioxidant capacity, total phenolic content, and antimicrobial properties against Staphylococcus aureus, Escherichia coli, and Candida albicans of grape vinegars were conducted. Moreover, gas chromatography was used to measure acetic acid content in the vinegars. The research material consisted of vinegars produced from five different grape varieties. For each variety, two variants were prepared: with and without the addition of sugar in the fermentation process. The highest antimicrobial activity against all micro-organisms was observed in vinegar produced from Solaris grapes with added sugar. The highest polyphenol content was observed in vinegar produced from the Prior grape variety with added sugar and the highest total antioxidant capacity is the Johanniter grape variety with added sugar. The vinegars examined in this study differed, depending on grape variety, in terms of antimicrobial properties, antioxidant capacity, total phenolic content, as well as acetic acid content. Sugar addition caused significant differences in the antioxidant capacity of vinegar samples.
Iloprost Attenuates Oxidative Stress-Dependent Activation of Collagen Synthesis Induced by Sera from Scleroderma Patients in Human Pulmonary Microvascular Endothelial Cells
Authors: Giordo Roberta , Thuan Duong Thi Bich , Posadino Anna Maria , Cossu Annalisa , Zinellu Angelo , Erre Gian Luca , Pintus Gianfranco
Published: August 2021

Endothelial cell injury is an early event in systemic sclerosis (SSc) pathogenesis and several studies indicate oxidative stress as the trigger of SSc-associated vasculopathy. Here, we show that circulating factors present in sera of SSc patients increased reactive oxygen species (ROS) production and collagen synthesis in human pulmonary microvascular endothelial cells (HPMECs). In addition, the possibility that iloprost, a drug commonly used in SSc therapy, might modulate the above-mentioned biological phenomena has been also investigated. In this regard, as compared to sera of SSc patients, sera of iloprost-treated SSc patients failed to increased ROS levels and collagen synthesis in HPMEC, suggesting a potential antioxidant mechanism of this drug.
Antifungal Activity of Isolated Compounds from the Leaves of Combretum erythrophyllum (Burch.) Sond. and Withania somnifera (L.) Dunal against Fusarium Pathogens
Authors: Seepe Hlabana Alfred , Ramakadi Tselane Geneva , Lebepe Charity Mekgwa , Amoo Stephen O. , Nxumalo Winston
Published: August 2021

Crop diseases caused by Fusarium pathogens, among other microorganisms, threaten crop production in both commercial and smallholder farming. There are increasing concerns about the use of conventional synthetic fungicides due to fungal resistance and the associated negative effects of these chemicals on human health, livestock and the environment. This leads to the search for alternative fungicides from nature, especially from plants. The objectives of this study were to characterize isolated compounds from Combretum erythrophyllum (Burch.) Sond. and Withania somnifera (L.) Dunal leaf extracts, evaluate their antifungal activity against Fusarium pathogens, their phytotoxicity on maize seed germination and their cytotoxicity effect on Raw 264.7 macrophage cells. The investigation led to the isolation of antifungal compounds characterized as 5-hydroxy-7,4′-dimethoxyflavone, maslinic acid (21-hydroxy-3-oxo-olean-12-en-28-oic acid) and withaferin A (4β,27-dihydroxy-1-oxo-5β,6β-epoxywitha-2-24-dienolide). The structural elucidation of the isolated compounds was established using nuclear magnetic resonance (NMR) spectroscopy, mass spectroscopy (MS) and, in comparison, with the available published data. These compounds showed good antifungal activity with minimum inhibitory concentrations (MIC) less than 1.0 mg/mL against one or more of the tested Fusarium pathogens (F. oxysporum, F. verticilloides, F. subglutinans, F. proliferatum, F. solani, F. graminearum, F. chlamydosporum and F. semitectum). The findings from this study indicate that medicinal plants are a good source of natural antifungals. Furthermore, the isolated antifungal compounds did not show any phytotoxic effects on maize seed germination. The toxicity of the compounds A (5-hydroxy-7,4′-dimethoxyflavone) and AI (4β,27-dihydroxy-1-oxo-5β,6β-epoxywitha-2-24-dienolide) was dose-dependent, while compound B (21-hydroxy-3-oxo-olean-12-en-28-oic acid) showed no toxicity effect against Raw 264.7 macrophage cells.
Recent Applications of Dual-Stimuli Responsive Chitosan Hydrogel Nanocomposites as Drug Delivery Tools
Authors: McCarthy Pumtiwitt C. , Zhang Yongchao , Abebe Fasil
Published: August 2021

Polysaccharides are a versatile class of macromolecules that are involved in many biological interactions critical to life. They can be further modified for added functionality. Once derivatized, these polymers can exhibit new chemical properties that can be further optimized for applications in drug delivery, wound healing, sensor development and others. Chitosan, derived from the N-deacetylation of chitin, is one example of a polysaccharide that has been functionalized and used as a major component of polysaccharide biomaterials. In this brief review, we focus on one aspect of chitosan’s utility, namely we discuss recent advances in dual-responsive chitosan hydrogel nanomaterials.
Composition Profiling and Authenticity Assessment of Camellia Oil Using High Field and Low Field 1H NMR
Authors: Xing Meijun , Wang Shenghao , Lin Jianzhong , Xia Feng , Feng Jianghua , Shen Guiping
Published: August 2021

Camellia oil (CA), mainly produced in southern China, has always been called Oriental olive oil (OL) due to its similar physicochemical properties to OL. The high nutritional value and high selling price of CA make mixing it with other low-quality oils prevalent, in order to make huge profits. In this paper, the transverse relaxation time (T2) distribution of different brands of CA and OL, and the variation in transverse relaxation parameters when adulterated with corn oil (CO), were assessed via low field nuclear magnetic resonance (LF-NMR) imagery. The nutritional compositions of CA and OL and their quality indices were obtained via high field NMR (HF-NMR) spectroscopy. The results show that the fatty acid evaluation indices values, including for squalene, oleic acid, linolenic acid and iodine, were higher in CA than in OL, indicating the nutritional value of CA. The adulterated CA with a content of CO more than 20% can be correctly identified by principal component analysis or partial least squares discriminant analysis, and the blended oils could be successfully classified by orthogonal partial least squares discriminant analysis, with an accuracy of 100% when the adulteration ratio was above 30%. These results indicate the practicability of LF-NMR in the rapid screening of food authenticity.
Unsymmetric Cisplatin-Based Pt(IV) Conjugates Containing a PARP-1 Inhibitor Pharmacophore Tested on Malignant Pleural Mesothelioma Cell Lines
Authors: Gabano Elisabetta , Pinton Giulia , Balzano Cecilia , Boumya Sara , Osella Domenico , Moro Laura , Ravera Mauro
Published: August 2021

Cisplatin is widely employed as a first-line chemotherapeutic agent for many solid tumors, including malignant pleural mesothelioma (MPM). However, its clinical use is limited by heavy side effects and acquired resistance, the latter being mainly related to enhanced DNA repair. Many clinical trials using combinations of platinum drugs and PARP-1 inhibitors (PARPis) have been carried out, with the hope that such combinations might lead to improved therapeutic efficacy against tumors. Here, the synthesis and efficacy in reducing MPM cell viability of four cisplatin-based Pt(IV) prodrugs containing the PARPi 3-aminobenzamide (3-ABA) fragment are described. The most promising conjugate is more effective than cisplatin or cisplatin/3-ABA combination, administered in equimolar doses, in inhibiting PARP-1 activity and inducing apoptosis in BRCA1/2 wild type MPM cells, grown as monolayer or as multicellular spheroids.
Quinoxaline Moiety: A Potential Scaffold against Mycobacterium tuberculosis
Authors: Montana Marc , Montero Vincent , Khoumeri Omar , Vanelle Patrice
Published: August 2021

Background. The past decades have seen numerous efforts to develop new antitubercular agents. Currently, the available regimens are lengthy, only partially effective, and associated with high rates of adverse events. The challenge is therefore to develop new agents with faster and more efficient action. The versatile quinoxaline ring possesses a broad spectrum of pharmacological activities, ensuring considerable attention to it in the field of medicinal chemistry. Objectives. In continuation of our program on the pharmacological activity of quinoxaline derivatives, this review focuses on potential antimycobacterial activity of recent quinoxaline derivatives and discusses their structure—activity relationship for designing new analogs with improved activity. Methods. The review compiles recent studies published between January 2011 and April 2021. Results. The final total of 23 studies were examined. Conclusions. Data from studies of quinoxaline and quinoxaline 1,4-di-N-oxide derivatives highlight that specific derivatives show encouraging perspectives in the treatment of Mycobacterium tuberculosis and the recent growing interest for these scaffolds. These interesting results warrant further investigation, which may allow identification of novel antitubercular candidates based on this scaffold.
Reduction of 3-Deoxyglucosone by Epigallocatechin Gallate Results Partially from an Addition Reaction: The Possible Mechanism of Decreased 5-Hydroxymethylfurfural in Epigallocatechin Gallate-Treated Black Garlic
Authors: Tu An-Ting , Lin Jer-An , Lee Chieh-Hsiu , Chen Yi-An , Wu Jung-Tsung , Tsai Ming-Shiun , Cheng Kuan-Chen , Hsieh Chang-Wei
Published: August 2021

5-Hydroxymethylfurfural (5-HMF) is a harmful substance generated during the processing of black garlic. Our previous research demonstrated that impregnation of black garlic with epigallocatechin gallate (EGCG) could reduce the formation of 5-HMF. However, there is still a lack of relevant research on the mechanism and structural identification of EGCG inhibiting the production of 5-HMF. In this study, an intermediate product of 5-HMF, 3-deoxyglucosone (3-DG), was found to be decreased in black garlic during the aging process, and impregnation with EGCG for 24 h further reduced the formation of 3-DG by approximately 60% in black garlic compared with that in the untreated control. The aging-mimicking reaction system of 3-DG + EGCG was employed to determine whether the reduction of 3-DG was the underlying mechanism of decreased 5-HMF formation in EGCG-treated black garlic. The results showed that EGCG accelerated the decrease of 3-DG and further attenuated 5-HMF formation, which may be caused by an additional reaction with 3-DG, as evidenced by LC-MS/MS analysis. In conclusion, this study provides new insights regarding the role of EGCG in blocking 5-HMF formation.
Diagnostic Measures of Disease Progression in Cattle Following Natural Infection with Bovine Leukemia Virus
Authors: Hutchinson Holden C. , Ruggiero Vickie J. , Norby Bo , Sporer Kelly R. B. , Bartlett Paul C.
Published: August 2021

This study describes the longitudinal changes in bovine leukemia virus (BLV) ELISA antibodies, proviral load (PVL), and blood lymphocyte counts (LC) observed over a 2.5-year period in naturally infected cattle. The dataset utilized was from a BLV intervention field trial on three Midwestern dairy herds. Our analysis showed ELISA false negatives were more likely to occur in cattle with low PVL and normal LC. On average, negligible changes in LC were observed during six-month intervals. Periods of lymphocytosis, defined as >10,000 lymphocytes per uL of blood, were observed in 31.5% (68/216) of BLV test-positive cattle. In BLV test-positive cows, an average increase of 2900 to 3100 proviral copies per 100,000 cells was observed during each subsequent six-month sampling interval. The difference between the minimum and maximum PVL observed for an ELISA-positive cow with 3 or more observations ranged from 0 to 115,600 copies per 100,000 cells (median: 12,900; mean: 19,200). Therefore, following the identification of ELISA-positive cattle and the assessment of PVL and LC, subsequent semiannual tests to assess disease progression may not be needed. Further work is needed to determine how available diagnostic tests can be optimized to design cost-effective testing schemes for BLV control programs.
β-Cells Different Vulnerability to the Parkinsonian Neurotoxins Rotenone, 1-Methyl-4-phenylpyridinium (MPP+) and 6-Hydroxydopamine (6-OHDA)
Authors: Carli Marco , Vaglini Francesca , Risaliti Eleonora , Citi Gianluca , Masini Matilde , Kolachalam Shivakumar , Maggio Roberto , Corsini Giovanni Umberto , Novelli Michela , De Tata Vincenzo , Scarselli Marco
Published: August 2021

Neurotoxins such as rotenone, 1-methyl-4-phenylpyridinium (MPP+) and 6-hydroxydopamine (6-OHDA) are well known for their high toxicity on dopaminergic neurons and are associated with Parkinson’s disease (PD) in murine models and humans. In addition, PD patients often have glucose intolerance and may develop type 2 diabetes (T2D), whereas T2D patients have higher risk of PD compared to general population. Based on these premises, we evaluated the toxicity of these three toxins on pancreatic β-cell lines (INS-1 832/13 and MIN6) and we showed that rotenone is the most potent for reducing β-cells viability and altering mitochondrial structure and bioenergetics in the low nanomolar range, similar to that found in dopaminergic cell lines. MPP+ and 6-OHDA show similar effects but at higher concentration. Importantly, rotenone-induced toxicity was counteracted by α-tocopherol and partially by metformin, which are endowed with strong antioxidative and cytoprotective properties. These data show similarities between dopaminergic neurons and β-cells in terms of vulnerability to toxins and pharmacological agents capable to protect both cell types.
Topical L-Ascorbic Acid Formulation for a Better Management of Non-Melanoma Skin Cancer: Perspective for Treatment Strategies
Authors: Capponi Pier Cesare , Murri Domenico , Pernice Carmine
Published: August 2021

L-ascorbic acid, is a well-known molecule, sometimes used as antioxidant for skin care. Nonetheless, few studies have taken in account its utility as topical treatment for non-melanoma skin vancer. Non-melanoma skin cancer includes basal cell carcinoma and squamous cell carcinoma and is widespread worldwide with an increasing incidence. The purpose of this paper is to analyze the characteristics of L-ascorbic acid topical formulation, its percutaneous absorption and biochemical mechanism, focusing on its anti-cancer properties. In particular, it will be described how the pH and the concentration of the formulation are able to influence its distribution in the skin and tissues. We will report, the current knowledge on the pharmacokinetic aspects of L-ascorbic acid that allows us to reconsider it in the light of its ability to act as a prodrug and as an anticancer agent. Lastly, a short review with the aim to find any evidence of a possible clinical use of L-ascorbic acid for the treatment of non-melanoma skin cancer was made.
Omega-3- and Resveratrol-Loaded Lipid Nanosystems for Potential Use as Topical Formulations in Autoimmune, Inflammatory, and Cancerous Skin Diseases
Authors: Caldas Ana R. , Catita José , Machado Raul , Ribeiro Artur , Cerqueira Fátima , Horta Bruno , Medeiros Rui , Lúcio Marlene , Lopes Carla M.
Published: August 2021

Resveratrol (RSV) and omega 3 (ω3), because of their biological favorable properties, have become subjects of interest for researchers in dermocosmetic and pharmaceutical industries; however, these bioactives present technological limitations that hinder their effective delivery to the target skin layer. To overcome the stability and skin permeation limitations of free bioactives, this work proposes a combined strategy involving two different lipid nanosystems (liposomes and lipid nanoparticles) that include ω3 in their lipid matrix. Additionaly, RSV is only encapsulated in liposomes that provid an adequate amphiphilic environment. Each formulation is thoroughly characterized regarding their physical–chemical properties. Subsequently, the therapeutic performance of the lipid nanosystems is evaluated based on their protective roles against lipid peroxidation, as well as inhibition of cicloxygenase (COX) and nitric oxid (NO) production in the RWA264.7 cell line. Finally, the lipid nanosystems are incorporated in hydrogel to allow their topical administration, then rheology, occlusion, and RSV release–diffusion assays are performed. Lipid nanoparticles provide occlusive effects at the skin surface. Liposomes provide sustained RSV release and their flexibility conferred by edge activator components enhances RSV diffusion, which is required to reach NO production cells and COX cell membrane enzymes. Overall, the inclusion of both lipid nanosystems in the same semisolid base constitutes a promising strategy for autoimmune, inflammatory, and cancerous skin diseases.
Antioxidative Effects of Carrot-Derived Nanovesicles in Cardiomyoblast and Neuroblastoma Cells
Authors: Kim Do Kyung , Rhee Won Jong
Published: August 2021

Oxidative stress is implicated in many diseases, including cardiovascular and neurodegenerative diseases. Because an increased level of oxidative stress causes apoptosis, it is necessary to inhibit cellular responses to oxidative stress. In this study, Carex, a nanovesicle from carrot, was isolated and investigated as a novel biomaterial with antioxidative function in cardiomyoblasts and neuroblastoma cells. A high concentration of nanovesicles was purified from carrots, using size-exclusion chromatography in combination with ultrafiltration. The characterization of Carex demonstrated that it had properties similar to those of extracellular vesicles. Carex showed low cytotoxicity in both H9C2 cardiomyoblasts and SH-SY5Y neuroblastoma cells, when a high level of Carex was delivered to the cells. Carex was further investigated for its antioxidative and apoptotic effects, and it significantly inhibited ROS generation and apoptosis in vitro in myocardial infarction and Parkinson’s disease models. Carex inhibited the reduction of antioxidative molecule expression, including Nrf-2, HO-1, and NQO-1, in both models. Considering its antioxidative function and high production yield, Carex is a potential drug candidate for the treatment of myocardial infarction as well as Parkinson’s disease. Thus, the results demonstrated in this study will contribute to an exploration of a novel drug, using nanovesicles from plants, including carrots.
Oligoarginine Peptide Conjugated to BSA Improves Cell Penetration of Gold Nanorods and Nanoprisms for Biomedical Applications
Authors: Bolaños Karen , Sánchez-Navarro Macarena , Tapia-Arellano Andreas , Giralt Ernest , Kogan Marcelo J. , Araya Eyleen
Published: August 2021

Gold nanoparticles (AuNPs) have been shown to be outstanding tools for drug delivery and biomedical applications, mainly owing to their colloidal stability, surface chemistry, and photothermal properties. The biocompatibility and stability of nanoparticles can be improved by capping the nanoparticles with endogenous proteins, such as albumin. Notably, protein coating of nanoparticles can interfere with and decrease their cell penetration. Therefore, in the present study, we functionalized albumin with the r8 peptide (All-D, octaarginine) and used it for coating NIR-plasmonic anisotropic gold nanoparticles. Gold nanoprisms (AuNPrs) and gold nanorods (AuNRs) were coated with bovine serum albumin (BSA) previously functionalized using a cell penetrating peptide (CPP) with the r8 sequence (BSA-r8). The effect of the coated and r8-functionalized AuNPs on HeLa cell viability was assessed by the MTS assay, showing a low effect on cell viability after BSA coating. Moreover, the internalization of the nanostructures into HeLa cells was assessed by confocal microscopy and transmission electron microscopy (TEM). As a result, both nanoconstructs showed an improved internalization level after being capped with BSA-r8, in contrast to the BSA-functionalized control, suggesting the predominant role of CPP functionalization in cell internalization. Thus, our results validate both novel nanoconstructs as potential candidates to be coated by endogenous proteins and functionalized with a CPP to optimize cell internalization. In a further approach, coating AuNPs with CPP-functionalized BSA can broaden the possibilities for biomedical applications by combining their optical properties, biocompatibility, and cell-penetration abilities.
Controlled Excitation of Supermodes in a Multicore Fiber with a 5 × 5 Square Array of Strongly Coupled Cores
Authors: Kalinin Nikolay A. , Anashkina Elena A. , Egorova Olga N. , Zhuravlev Sergey G. , Semjonov Sergei L. , Kim Arkady V. , Litvak Alexander G. , Andrianov Alexey V.
Published: August 2021

Coherent propagation of supermodes in a multicore fiber is promising for power scaling of fiber laser systems, eliminating the need for the active feedback system to maintain the phases between the channels. We studied the propagation of broadband pulsed radiation at a central wavelength of 1030 nm in a multicore fiber with coupled cores arranged in a square array. We designed and fabricated a silica multicore fiber with a 5 × 5 array of cores. For controllable excitation of a desired supermode, we developed a beam-forming system based on a spatial light modulator. We experimentally measured intensity and phase distributions of the supermodes, in particular, the in-phase and out-of-phase supermodes, which matched well the numerically calculated profiles. We obtained selective excitation and coherent propagation of broadband radiation with the content of the out-of-phase supermode of up to 90% maintained without active feedback. Using three-dimensional numerical modeling with allowance for a refractive index profile similar to those of the developed fiber, we demonstrated stable propagation of the out-of-phase supermode and collapse of the in-phase supermode at a high signal power.
Geometric Interpretation and General Classification of Three-Dimensional Polarization States through the Intrinsic Stokes Parameters
Authors: Gil José J.
Published: August 2021

In contrast with what happens for two-dimensional polarization states, defined as those whose electric field fluctuates in a fixed plane, which can readily be represented by means of the Poincaré sphere, the complete description of general three-dimensional polarization states involves nine measurable parameters, called the generalized Stokes parameters, so that the generalized Poincaré object takes the complicated form of an eight-dimensional quadric hypersurface. In this work, the geometric representation of general polarization states, described by means of a simple polarization object constituted by the combination of an ellipsoid and a vector, is interpreted in terms of the intrinsic Stokes parameters, which allows for a complete and systematic classification of polarization states in terms of meaningful rotationally invariant descriptors.
Challenges of Turning the Sargassum Crisis into Gold: Current Constraints and Implications for the Caribbean
Authors: Oxenford Hazel A. , Cox Shelly-Ann , van Tussenbroek Brigitta I. , Desrochers Anne
Published: August 2021

Over the last decade, the Caribbean has seen massive, episodic influxes of pelagic sargassum negatively impacting coastal ecosystems, people’s livelihoods and climate-sensitive sectors. Addressing this issue solely as a hazard has proven extremely costly and attention is slowly turning towards the potential opportunities for sargassum reuse and valorization. However, turning the ‘sargassum crisis into gold’ is not easy. In this study we use a multi-method approach to learn from sargassum stakeholders (researchers, entrepreneurs and established businesses) across the Caribbean about the constraints and challenges they are facing. These can be grouped into five broad categories: (1) unpredictable supply of sargassum; (2) issues related with the chemical composition of the seaweed; (3) harvest, transport and storage; (4) governance; and (5) funding. Specific issues and potential solutions associated with each of these categories are reviewed in detail and recommended actions are mapped to five entry points along a generalized value chain to demonstrate how these actions can contribute to the development of sustainable sargassum value chains that promote economic opportunities and could help alleviate impacts of massive influxes. This paper offers guidance to policy makers and funding agencies on existing gaps and challenges that need to be addressed in order to scale-up successful and sustainable solutions to the sargassum crisis.
The Steroid Saponin Protodioscin Modulates Arabidopsis thaliana Root Morphology Altering Auxin Homeostasis, Transport and Distribution
Authors: Santos Wagner Ana Luiza , Araniti Fabrizio , Bruno Leonardo , Ishii-Iwamoto Emy Luiza , Abenavoli Maria Rosa
Published: August 2021

To date, synthetic herbicides are the main tools used for weed control, with consequent damage to both the environment and human health. In this respect, searching for new natural molecules and understanding their mode of action could represent an alternative strategy or support to traditional management methods for sustainable agriculture. Protodioscin is a natural molecule belonging to the class of steroid saponins, mainly produced by monocotyledons. In the present paper, protodioscin’s phytotoxic potential was assessed to identify its target and the potential mode of action in the model plant Arabidopsis thaliana. The results highlighted that the root system was the main target of protodioscin, which caused a high inhibitory effect on the primary root length (ED50 50 μM) with morphological alteration, accompanied by a significant increase in the lateral root number and root hair density. Through a pharmacological and microscopic approach, it was underlined that this saponin modified both auxin distribution and transport, causing an auxin accumulation in the region of root maturation and an alteration of proteins responsible for the auxin efflux (PIN2). In conclusion, the saponin protodioscin can modulate the root system of A. thaliana by interfering with the auxin transport (PAT).
Influence of Flame Retardant Impregnation on Acoustic and Thermophysical Properties of Recycled Technical Textiles with the Potential for Use in Wooden Buildings
Authors: Danihelová Anna , Sčensný Patrik , Gergeľ Tomáš , Ondrejka Vojtěch , Němec Miroslav , Igaz Rastislav , Štefko Jozef , Mitterová Iveta
Published: August 2021

This article presents the results of an investigation of acoustic and thermophysical properties of insulation panels made from recycled technical textiles originating from the automotive industry. Measurements were performed on the samples of insulation panels (Senizol AT XX2 TL60), which were modified with liquid flame retardants (ISONEM® ANTI-FIRE SOLUTION, ECOGARD® B45, HR Prof). Another method of treatment was carried out by surface application of non-flammable facing (woven carbon fibre, nonwoven carbon fibre). Retardants were applied to the samples by surface spraying and soaking. The results showed a high ability of material to absorb sound in the frequency range 350 Hz–2 kHz. The sound absorption coefficient ranged from 0.82 to 0.9 in the frequency range 500 Hz–2 kHz. The noise reduction coefficient is 0.75. After material modification with the flame retardants, there was no significant change of sound absorption. The thermal conductivity coefficient of material before modification was 0.038 W⋅m−1⋅K−1. After application of the flame retardants, the thermal conductivity coefficient increased depending on type and method of retardant application in the range of 2.6–105.3%. The smallest change was detected after modification of material with ECOGARD® B45.
Enhanced Impact Properties of Hybrid Composites Reinforced by Carbon Fiber and Polyimide Fiber
Authors: Wang Boyao , He Bin , Wang Zhanwen , Qi Shengli , Zhang Daijun , Tian Guofeng , Wu Dezhen
Published: August 2021

A series of hybrid fiber-reinforced composites were prepared with polyimide fiber and carbon fiber as the reinforcement and epoxy resin as the matrix. The influence of stacking sequence on the Charpy impact and flexural properties of the composites as well as the failure modes were studied. The results showed that hybrid fiber-reinforced composites yielded nearly 50% increment in Charpy impact strength compared with the ones reinforced by carbon fiber. The flexural performance was significantly improved compared with those reinforced solely by polyimide fibers and was greatly affected by the stacking sequence. The specimens with compressive sides distributed with carbon fiber possessed higher flexural strength, while those holding a sandwich-like structure with carbon fiber filling between the outer layers displayed a higher flexural modulus.
Development of Antiviral CVC (Chief Value Cotton) Fabric
Authors: Wang Wen-Yi , Yim Sui-Lung , Wong Chun-Ho , Kan Chi-Wai
Published: August 2021

The outbreak of COVID-19 has already generated a huge societal, economic and political losses worldwide. The present study aims to investigate the antiviral activity of Poly(hexamethylene biguanide) hydrochloride (PHMB) treated fabric against COVID-19 by using the surrogate Feline coronavirus. The antiviral analysis indicated that up to 94% of coronavirus was killed after contacting the CVC fabric treated with PHMB for 2 h, which suggests that PHMB treated fabric could be used for developing protective clothing and beddings with antiviral activity against coronavirus and can play a role in fighting the transmission of COVID-19 in the high-risk places.
Protein-, (Poly)peptide-, and Amino Acid-Based Nanostructures Prepared via Polymerization-Induced Self-Assembly
Authors: Varlas Spyridon , Maitland Georgia L. , Derry Matthew J.
Published: August 2021

Proteins and peptides, built from precisely defined amino acid sequences, are an important class of biomolecules that play a vital role in most biological functions. Preparation of nanostructures through functionalization of natural, hydrophilic proteins/peptides with synthetic polymers or upon self-assembly of all-synthetic amphiphilic copolypept(o)ides and amino acid-containing polymers enables access to novel protein-mimicking biomaterials with superior physicochemical properties and immense biorelevant scope. In recent years, polymerization-induced self-assembly (PISA) has been established as an efficient and versatile alternative method to existing self-assembly procedures for the reproducible development of block copolymer nano-objects in situ at high concentrations and, thus, provides an ideal platform for engineering protein-inspired nanomaterials. In this review article, the different strategies employed for direct construction of protein-, (poly)peptide-, and amino acid-based nanostructures via PISA are described with particular focus on the characteristics of the developed block copolymer assemblies, as well as their utilization in various pharmaceutical and biomedical applications.
Impact of Various Vacuum Impregnation Methods on Viability of Cereal Grains
Authors: Kobus Zbigniew , Rydzak Leszek , Guz Tomasz , Domin Marek , Nadulski Rafał , Osmólska Emilia
Published: August 2021

Grain viability is of key importance in the production of malt. This paper presents the results of research on the influence of vacuum impregnation of grains of various cereals on theirservice life. One malting barley cultivar, two wheat cultivars, and a rye cultivar mix were tested. The grain was subjected to wet vacuum impregnation and wet vacuum impregnation carried out cyclically under the pressure of 5 kPa. Then, samples of the impregnated grain were sown and its germination capacity was tested every 24 h to 120 h. The control sample was grain soaked under atmospheric pressure. Vacuum impregnation did not cause any significant changes in the germination capacity of the examined cereal varieties. The vacuum-impregnated grain displayed a higher initial water content and germinated faster. It was found that the impregnation did not disturb the internal structure of the grain, indicated by the fact that the most delicate part of the grain, i.e., the embryo, retained its vital functions in every examined case.
Advances in Microbial Fermentation Processes
Authors: Tufariello Maria , Grieco Francesco
Published: August 2021

In the food sector, fermentation processes have been the object of great interest in regard to enhancing the yield, the quality, and the safety of the final product [...]