bluuebunny/crossref_metadata_2025 huggingface dataset:
An immune signature for risk stratification and therapeutic prediction in helicobacter pylori-infected gastric cancer
Authors: Geng haigang , Dong Zhongyi , Zhang Linmeng , Yang Chen , Li Tingting , Lin Yuxuan , Ke Shouyu , Xia Xiang , Zhang Zizhen , Zhao Gang , Zhu Chunchao
Published: June 2022

Helicobacter pylori (HP) infection is the greatest risk factor for gastric cancer (GC). Increasing evidence has clarified that tumor immune microenvironment (TIME) is closely related to the prognosis and therapeutic efficacy of HP-positive (HP+) GC patients. In this study, we aimed to construct a novel immune-related signature for predicting the prognosis and immunotherapy efficacy of HP + GC patients. A total of 153 HP + GC from three different cohorts were included in this study. An Immune-Related prognostic Signature for HP + GC patients (IRSHG) was established using Univariate Cox regression, the LASSO algorithm, and Multivariate Cox regression. Univariate and Multivariate analyses proved IRSHG was an independent prognostic predictor for HP + GC patients, and an IRSHG-integrated nomogram was established to quantitatively access the prognostic risk. The low-IRSHG group exhibited higher copy number load and distinct mutation profiles compared with the high-IRSHG group. In addition, the difference of hallmark pathways and immune cells infiltration between the two groups was investigated. Notably, tumor immune dysfunction and exclusion (TIDE) analysis indicated that the low-IRSHG group had a higher sensitivity to anti-PD-1 immunotherapy, which was validated by an external pabolizumab treatment cohort. Besides, 98 chemotherapeutic drugs and corresponding potential biomarkers were identified for two groups, and several drugs with potential ability to reverse IRSHG score were identified using CMap analysis. Collectively, IRSHG may serve as a promising biomarker for survival outcome as well as immunotherapy efficacy. Furthermore, it can also help to prioritize potential therapeutics for HP + GC patients, providing new insight for the personalized treatment of HP-infected GC.
Identification of Violent Response using Feature Extraction Matrix Algorithm of a Time Series Data (FEM)
Authors: Randhawa Princy , Shanthagiri Vijay , Alharbi Hadeel Fahad , Patel Akshet
Published: June 2022

There are several women safety devices in the market today. Women who suffer these atrocities are even denied basic human rights, as set out in the Criminal Code. Women who are not as fit (physically) as men need to be protected from the evils of society. The introduction of actions and procedures for healthier women is not adequate and needs to be well improved. However, these devices are not fool- proof. The crux of this paper is a partial result of a challenging problem faced during design and construction a fool-proof Smart Jacket for women’s safety using fabric sensors. The smart jacket is envisioned for the women to wear on all occasions. An alert message and subjects’ Geo-location is sent to pre-assigned phone number if the subject is faced with violent situation. The jacket consists of fabric Sensors, Accelerometer, Gyroscope and Magnetometer, which are strategically placed to record maximum variations in signal for minimum movement in subject’s body. The primary challenge is not in design or construction of a jacket, but in accurately classifying violent activity from animated activity. Both violent activity and animated activities have commonality in sensor excitation. There are subtle differences which needs to be extracted to train a Machine learning algorithm to learn these patterns. Multivariate Regression Analysis (MRA) is used to highlight explanatory variables that can produce better convergence. With MRA results signifying a high degree of noise in the data, a newer approach, wherein, the ordinality of the data was ignored and cardinality of the data was considered for analysis. Machine learning approaches is then used to build a model using features extracted through MRA. Different machine learning techniques have been applied to classify the violent attack from the different activities such as stationary subject, walking, dancing. This study present that Support vector machine shows the better classification accuracy, computation time as compared to other algorithms.
Security Provisions in Smart Edge Computing Devices using Blockchain and Machine Learning Algorithms: A Novel Approach
Authors: Mishra Kamta Nath , Bhattacharjee Vandana , Saket Shashwat , Mishra Shivam P.
Published: June 2022

The security provisions for capricious data in a dynamic environment must be fathomed by prudence. It is strenuous to manage gigantic data in an ossified environment through one server. The authors have put their concern on the security risk on the susceptible data in a Mobile Edge distributed environment. Thus, edge computing turns out to be an excellent perspective in which the training can be done at the Edge-based environment. The amalgamation of Edge computing and Blockchain in association with machine learning techniques can foster data security, mitigate the risk of exposed data, and further alleviate the risk of a data breach. Thus, the concept of federated learning provides an itinerary for training the shared data. The dataset containing several vulnerable, exposed, recovered, and secured data were collected. And the security of data was precepted under the surveillance of two-factor authentication. This paper discusses the metamorphism of data and security breaches existing in smart edge computing devices. The proposed model consists of the implication of data security using Blockchain and machine learning techniques comprising several classifiers and optimization techniques. They further effectuated the algorithm in an edge computing-based environment by apportioning several batches of data amongst different clients. Subsequently, the authors-maintained privacy for the clients using Blockchain servers. They segregated the data from the clients into batches trained using the federated learning technique. The aftermaths procured in this paper proclaim the implementation of a Blockchain-based training model in the edge-based ecosystem.
Several techniques for introducing memory in iterative processes to solve nonlinear systems
Authors: Cordero Alicia , Garrido Neus , Torregrosa Juan R. , Triguero-Navarro Paula
Published: June 2022

In this paper, we design two parametric classes of iterative methods without memory to solve nonlinear systems, whose convergence order is four and seven, respectively. From their error equations and to increase the convergence order without performing new functional evaluations, memory is introduced in these families of different forms. That allows us to increase from four to seven the convergence order in the first family and from seven to eleven in the second one. We perform some numerical experiments with big size systems for confirming the theoretical results and comparing the proposed methods along other known schemes.
Heavy Metals Assessment of Ecosystem Polluted with Wastewaters and Taxonomic Profiling of Multi-Resistant Bacteria with Potential for Petroleum Hydrocarbon Catabolism in Nitrogen-Limiting Medium
Authors: Oyetibo Ganiyu Oladunjoye , Adebusoye Sunday Adekunle , Ilori Matthew Olusoji , Amund Olukayode Oladipo
Published: June 2022

The coexistence of heavy metals (HMs) and petroleum hydrocarbons (PHs) exacerbates ecotoxicity and impair the drivers of eco-functionalities that stimulate essential nutrients for the productivity of the impacted environment. Profiling the bacteria that stem the ecological impact via HMs sequestration and PHs catabolism with nitrogen fixation is imperative to bioremediation of the polluted sites. The sediment of site that was consistently contaminated with industrial wastewaters was analysed for ecological toxicants and the bacterial strains that combined HMs resistance with PHs catabolism in a nitrogen-limiting system were isolated from the sediment and characterized. The geochemistry of the samples revealed the co-occurrence of the above-benchmark concentrations of HMs with the derivatives of hydrocarbons. Notwithstanding, nickel and mercury (with 5% each of the total metal concentrations in the polluted site) exhibited probable effect concentrations on the biota and thus hazardous to the ecosystem. Approx. 31% of the bacterial community, comprising unclassified Planococcaceae, unclassified Bradyrhizobiaceae, Rhodococcus, and Bacillus species, resisted 160 µmol Hg2+ in the nitrogen-limiting system within 24 h post-inoculation. The bacterial strains adopt volatilization, and sometimes in combination with adsorption/bioaccumulation strategies to sequester Hg2+ toxicity while utilizing PHs as sources of carbon and energy. Efficient metabolism of petroleum biomarkers (> 87%) and Hg2+ sequestration (≥ 75% of 40 µmol Hg2+) displayed by the selected bacterial strains portend the potential applicability of the bacilli for biotechnological restoration of the polluted site.
Analysis of an Unsteady Incompressible Crossflow on a Stationary Circular Cylinder at Reynolds number 3,900 Using Refined Potential Flow Theory
Authors: Amoloye Taofiq O.
Published: June 2022

The motion of a fluid around a circular cylinder presents interesting phenomena including flow separation, wake and turbulence. The physics of these are enshrined in the continuity equation and the Navier-Stokes Equations (NSE). Therefore, their studies are important in mathematics and physics. They also have engineering applications. These studies can either be carried out experimentally, computationally, or theoretically. Theoretical studies of a cylinder flow using classical potential flow theory (CPT) have some gaps when compared to experiments. Attempting to bridge these gaps, this article introduces refined potential flow theory (RPT) and employs it on a stationary circular cylinder incompressible crossflow at Reynolds number 3,900. It leverages experimental observations, physical deductions and some agreements between CPT and experiments in the theoretical development. This results in the incompressible Eulerian Kwasu function which is a quasi-irrotational stream function that satisfies the governing equations and boundary conditions. It captures vorticity, boundary layer, shed wake vortices, three-dimensional effects, and static unsteadiness. The Lagrangian form of the function is exploited for the flow pathlines that are used to incorporate dynamic unsteadiness. A gravity analogy is used to predict the separation, transition, and reattachment points. The analogy introduces the perifocal frame of fluid motion. The forces are obtained in this frame with a change of variable. The drag prediction is within the error bound of measured data. The RPT pressure distribution, separation point and Strouhal number are also within acceptable ranges. Energy spectra analyses of the wake velocity display Kolmogorov’s Five-Thirds law of homogeneous isotropic turbulence.
CASSI - AN OPTIMAL ZEROS ASSIGNMENT METHOD FOR SOLVING ASSIGNMENT PROBLEMS
Authors:   Associate Professor, Department of Mathematics, St. Johns College, Palayamkottai-627002., Murugesan R.
Published: June 2022

An assignment problem (AP) is a meticulous case ofa transportation problem, in which the goal is to allocate a number of facilities to an equal number of activities at an overall maximum profit (or minimum cost, distance, time). It occupies a verysignificantrole in the real physical world. The well-known method applied to solve the APs is the Hungarian method, which generates optimal solution to a given AP. A little bit difficulty in the Hungarian method is to cover all the zero entries of a reduced cost matrix using minimum number of horizontal and vertical lines. However, this task has been made easy if one applies the ME Rulespresented in the Mantra technique. In this research article, we make an attempt to bring in a new technique named asCASSIfor obtaining an optimal solutionto anygiven AP using an optimality testing and improving technique. The added advantage of this method is that for any AP, the solution obtained by applying any method based on zeros assignment approach can be tested for optimality and can also be improved towards optimal, if its not optimal.
COMPARATIVE EVALUATION OF MICROLEAKAGE AND RETENTION OF DIFFERENT LUTING AGENTS USED FOR CEMENTING CUSTOM MADE STAINLESS STEEL BANDS ON MOLARS-AN IN VITRO STUDY
Authors:   Post Graduate student,Department of Pediatric and Preventive Dentistry, Divya Jyoti College of Dental Science and Research, Modinagar, Ghaziabad, Uttar Pradesh, India., Renuka Oinam , Somani Rani ,   Professor and head, Department of Pediatric and Preventive Dentistry, Divya Jyoti College of Dental Science and Research, Modinagar,Ghaziabad., Basu Payel ,   Post Graduate student,Department of Pediatric and Preventive Dentistry, Divya Jyoti College of Dental Science and Research, Modinagar, Ghaziabad, Uttar Pradesh, India., Bashir Arwah ,   Post Graduate student,Department of Pediatric and Preventive Dentistry, Divya Jyoti College of Dental Science and Research, Modinagar, Ghaziabad, Uttar Pradesh, India., Leleesh Mayanglambam ,   Post Graduate student,Department of Pediatric and Preventive Dentistry, Divya Jyoti College of Dental Science and Research, Modinagar, Ghaziabad, Uttar Pradesh, India., Thanglienzo Grace ,   Post Graduate student,Department of Pediatric and Preventive Dentistry, Divya Jyoti College of Dental Science and Research, Modinagar, Ghaziabad, Uttar Pradesh, India.
Published: June 2022

Aim: The aim of this study was to evaluate the microleakage and retentive strength of various luting agents. Material And Method: Forty samples were randomly divided into group A (assessment of microleakage of different luting agents ) and B (assessment of the retentive strength of the different luting agents) with 20 samples each. Both Group A ( assessment of microleakage of different luting agents) and B (assessment of the retentive strength of the different luting agents) were further subdivided into group A1, A2, A3 and A4 & B1, B2, B3 and B4 respectively with 5 samples each. Group A1, B1- ZINC PHOSPHATE( CONTROL GROUP )Group A2, B2 - SPEED CEM PLUS Group A3, B3 - HY BOND CX SMART GLASIONOMER Group A4, B4- NO CEMENT ( NEGATIVE GROUP) Results: Speed CEM Plus has the lowest mean value while zinc phosphate has the highest mean value of microleakage. While it was noted that Speed CEM Plus had the highest mean value while zinc phosphate has the lowest mean value of retentive strength Conclusion: Speed CEM Plus can be recommended as a potential and efficiency luting cement material in term of lower microleakage and high retentive strength for cementing stainless steel band on molar.
INFLUENCE OF RIGHT TO EDUCATION ON PERFORMANCE OF TEACHERS IN SELECT SCHOOLS IN BANGALORE CITY
Authors:   Research Scholar, K.S.R College of Arts & Sciences Periyar University- India., Surendar G. , Bhatt Krishnan ,   Adjunct. Professor, Kristu Jayanthi College, Bangalore.
Published: June 2022

The Right of Children to Free and Compulsory Education Act or Right to Education Act (RTE), enacted by the Parliament of India on 4 August 2009, paves way for compulsory basic education to children across India. This act helps to uplift the society by assigning more academic responsibilities to schools and teachers. Therefore, right to education will increase the workload and have strong effect on performance of teachers in schools. This study focuses on the impact in performance of teachers and measures to overcome the same. Descriptive research method was adopted utilizing both primary and secondary data. This way, the present study is conducted with a sample of 100 teachers working in select schools in Bangalore City, India. Personal interaction and useful questionnaires answered by teachers provided the data for analysis. The questionnaire comprised four parts, such as demographic profile, impact of RTE in classroom teaching, role of training and development in performance of teachers, and expectations of teachers in workplace. The study used simple percentage analysis, factor analysis, multiple regressions analysis, Friedmans chi-square test and Garret ranking analysis, to collate and analyze the data. This study results reveal that RTE admissions create classroom management challenges and impact performances of teachers. The study results further reveal that in order to overcome the challenge and enhance the performance of teachers and increase academic outcomes, performance management, training and development programs and meeting the at par remuneration, at par workload and resource accessibility expectations of teachers are all equally significantly important.
REINFORCING AND ENABLING FACTORS INVOLVED IN THE UPTAKE OF SICKLE-CELL DISEASE SCREENING AMONG COLLEGE OF EDUCATION STUDENTS IN NIGERIA
Authors:   Department of Public Health, Babcock University, Ilishan-Remo, Ogun State, Nigeria., Adebusola O. Oyeyemi , Nnodimele O. Atulomah ,   Department of Public Health, Babcock University, Ilishan-Remo, Ogun State, Nigeria.
Published: June 2022

Introduction: Sickle cell disease (SCD) is one of the most common hereditary diseases occurring worldwide, which may affect any organ or system of human body. Some studies have shown that the knowledge of SCD and genetic screening among unmarried youths is low in Sub-Saharan Africa countries. However, genetic counseling offers help and guidance to individuals who may have or are at the risk of having genetic diseases based on their genetic information, including their family background. Aims and Objectives: This study assessed the social support and enabling factors towards sickle cell screening and counseling uptake among students Materials and Methodology: The cross-sectional study was carried out among students of Emmanuel Alayande College of Education, Oyo and Osun State College of Education, Ilesha. Self-administered questionnaire was administered to 612 participants and 4 FGD was conducted in both study sites. Data was analyzed using SPSS software. Result: Most (60.9%) and (70.8%) of the respondents were female in both Emmanuel Alayande College of Education (EACE) and Osun State College of Education (OSCE) respectively and respondents aged between 15-30 years. Almost half (47.7%) of respondents in EACE and some (21.8%) of OSCE respondents have not gone for their genotype testing. In the OSCE, most of the FG participants were of the opinion that going for SCD screening is not common due to fear of the result and the effects it may have on them. Most (64.1%) and (69.8%) of the respondent from EACE and OSCE respectively said that support from health care providers is very important in influencing their uptake of SCD screening. In both EACE and OSCE, FGD participants highlighted lack of proper screening centers, distance of such centers, as well as poor attitude of health workers as factors that can hinder the uptake of SCD screening. Conclusion: The findings from the study revealed that almost half of respondents in EACE and some of OSCE respondents have not gone for their genotype testing. A greater percentage of the respondents stated the need for social support including support from health care providers, friends, families, religious leaders as very important. There should be a collaboration between governmental and non-governmental health organizations to facilitate the promotion of free genotype screening and promote access to genetic counseling in public hospitals for young people.
A RARE CASE OF TUBERCULAR LYMPHO-TRACHEAL FISTULA
Authors:   Department of Pulmonary Medicine, Eras Lucknow Medical College & Hospital, Era University, Lucknow, Uttar Pradesh, India., Prasad Rajendra , Shamim Huda ,   Department of Pulmonary Medicine, Eras Lucknow Medical College & Hospital, Era University, Lucknow, Uttar Pradesh, India., Kacker Rishabh ,   Department of Pulmonary Medicine, Eras Lucknow Medical College & Hospital, Era University, Lucknow, Uttar Pradesh, India., Gupta Nikhil ,   Department of Medicine, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
Published: June 2022

Tuberculosis can affect any organ of the body. Tracheal manifestations due to mycobacterium tuberculosis are unusual and lympho-tracheal fistula exceptionally rare. In such rare cases early diagnosis and management is often delayed and may lead to complications. To the best of our knowledge, this rare case represents the first report of successful treatment of tuberculous lympho-tracheal fistula with anti-tubercular drugs with strict control of Diabetes mellitus.
PROSPECTIVE STUDY OF MICROBIOLOGY OF BIOFILM IN ORTHOPAEDIC IMPLANT SURGERY
Authors:   Associate Professor, Dept. of Orthopaedics, NSCB Medical College, Jabalpur, Madhya Pradesh., Tirkey Rakesh , Vidyarthi Ashok ,   Professor& Head, Dept. of Orthopaedics, NSCB Medical College, Jabalpur, Madhya Pradesh., Jain Seth Riti ,   Professor& Head, Dept. of Microbiology, NSCB Medical College, Jabalpur, Madhya Pradesh., Kumar Himanshu Sujeet ,   Corresponding author, PG Resident, Dept. of Orthopaedics, NSCB Medical College, Jabalpur, Madhya Pradesh.
Published: June 2022

Background:Infection remains a major obstacle leading to implant failure, increased morbidity, and mortality. Implant-relatedinfections continue to pose a problem for orthopaedicians. In spite of the decreasing incidence of orthopaedic device-related infections to 1%, nowadays, device-related infections still remain a diagnostic, therapeutic, and cost-related problem. Aims And Objective: To isolate and identify the Bacteriological agent forming Biofilm in orthopaedic implant infection and their antibiotic sensitivity. Methods: In a prospective study, 100 patients who underwent orthopaedic implant removal surgery from Dec 2019 â€“ to Sept 2021 were enrolled82 patients culture reports came out positive. Results: Of the 100 samples, 82(82%) were culture positive, and 18(18%) were culture negative. The femur was the most commonly affected bone in both males (median ageâ€“37.1yrs) and females (median ageâ€“41.3 yrs). Pseudomonas Aeruginosa was the organism that was most commonly isolated, followed by Staphylococcus Aureus and E.coli. No anaerobes were isolated. Aggressive antibiotic therapy was often found inadequate to eliminate the infections caused by the biofilm-forming organism. Conservative surgical treatment was associated with treatment failures. Conclusion: The most common bacteria which were isolated included Pseudomonas aeruginosa, followed by Staphylococcus aureus and E coli. A majority of them are resistant to the commonly used antibiotics, leading to treatment failures that necessitated implant removal.
A STUDY OF THE LEVELS OF SERUM MAGNESIUM AND SERUM ZINC IN PATIENTS OF SUBCLINICAL HYPOTHYROIDISM
Authors:   Msc 3rd year student, Department of Biochemistry, School of Medical Sciences and Research and Sharda Hospital,Sharda University, Greater Noida, Uttar Pradesh, India., Abdulsalam A.A.Al-Azazi Thuraya , Verma Shaliza ,   Associate Professor, Department of Biochemistry, School of Medical Sciences and Research and Sharda Hospital,Sharda University, Greater Noida, Uttar Pradesh, India., Kr Thakur Rajesh ,   Assistant Professor, Department of Biochemistry, School of Medical Sciences and Research and Sharda Hospital,Sharda University, Greater Noida, Uttar Pradesh, India., Kumar Nandkeoliar Manoj ,   Professor, Department of Biochemistry, School of Medical Sciences and Research and Sharda Hospital,Sharda University, Greater Noida, Uttar Pradesh, India., Punia V.P.S ,   Professor, Department of Medicine, School of Medical Sciences and Research and Sharda Hospital,Sharda University, Greater Noida, Uttar Pradesh, India., Kabi BC ,   Professor, Department of Biochemistry, School of Medical Sciences and Research and Sharda Hospital,Sharda University, Greater Noida, Uttar Pradesh, India.
Published: June 2022

Background: Subclinical Hypothyroidism (SCH), according to the endocrine web is defined as an elevationin in Thyroid Stimulating Hormone (TSH) level (4.6-10 m I U/L) with thyroid hormone levels in the normal range. Magnesium (Mg2+) is an essential macro-mineral in human body, which acts as a cofactor for more than 300 enzymes. Zinc (Zn+2) is required for proper functioning of 5-deiodinase & it is a cofactor for Thyrotropin -Releasing Hormone (TRH). Transcription factorsbind to thyroid hormones which are fundamental for modulation expression of gene, also contain Zn2+ bound to cysteine residues. Aim and objectives: This study aim to estimate the levels of serum magnesium and zinc in patients of SCH. Material and methods: The serum TSH, Total Triiodothyronine (T3)& Thyroxine (T4) levels were estimated by ELISA method to identify the cases of SCH. The concentration of serum magnesium was analyzed using semiautomated analyzerbyspectrophotometry. Serum zinc was analyzed by fully automated analyzer (Vitros 5600) in patients of SCH. Results:In SCH, both T3, T4 were within the normal range and TSH in the range of 4.6-10 m I U/L, there was a statistically significant (p<0.001) decrease in serum zinc levels and not significantly decrease in serum magnesium levels in subclinical hypothyroidism patients.
EVALUATION OF NAPSIN A EXPRESSION IN LUNG CANCERS AND ITS COMPARISON WITH THYROID TRANSCRIPTION FACTOR 1
Authors:   Assistant professor of Pathology, Govt. Karur Medical College, Karur., Manibarathi R. , Suganya U. ,   Assistant professor of Pathology, Govt. Mohan Kumaramangalam Medical College, Salem., Suganthi C. ,   Assistant Professor of Pathology, Govt. Dharmapuri Medical College, Dharmapuri.
Published: June 2022

Introduction: Lung cancer is the leading cause of cancer mortality in the world. With the development of new, successful treatments for adenocarcinoma, it is essential to subtype Non- Small Cell Lung Carcinoma whenever possible. Thyroid Transcription Factor-1 is a promising marker for lung adenocarcinoma but its sensitivity and specificity is limited. Napsin A is a new marker that may be useful as a part of immunohistochemistry panel to classify poorly differentiated lung carcinoma on small biopsies. Aim: To study the expression of Napsin A in lung cancer and to compare it with TTF-1 that has already recognized as a useful marker for lung adenocarcinoma. Methodology: This is a retrospective study. By analyzing the histopathology records during the period of 2015 - 2017, histopathological slides of biopsy proven 50 malignant lung cases were collected. Napsin A expression was tested and compared with TTF1. Results: Adenocarcinoma is the commonest type. Most common age group is 51-60 years with male female ratio is 2.7:1. Smokers have increased risk for malignancy. Napsin A and TTF1 showed similar sensitivity and the specificity of Napsin A was higher than TTF1 to lung adenocarcinoma. Napsin A showed higher sensitivity than TTF1 to exclude lung small cell carcinoma. Conclusion: Napsin A is almost limited to adenocarcinoma, whereas TTF-1 is not so specific. Although TTF-1 is expressed in majority of adenocarcinomas, its expression is also noted in most of the small-cell lung carcinomas. Hence Napsin A can be used as an exclusionary marker for small cell carcinoma. Key words: Lung cancer, Napsin A, Small biopsy, Thyroid transcription factor 1.
THE ROLE OF HYSTEROLAPAROSCOPY OVER HYSTEROSALPINGOGRAPHY IN THE EVALUATION AND MANAGEMENT OF FEMALE INFERTILITY: A DIAGNOSTIC-CUM FERTILITY ENHANCING THERAPEUTIC TOOL
Authors:   Professor, Department of Obstetrics & Gynecology, Smt. KashibaiNavale Medical College, Narhe, Pune-411041(Maharashtra)., K. Junnare Ketaki , Singh Shekhawat Gulab ,   Professor& HOD, Department of Obstetrics & Gynecology, Smt. Kashibai Navale Medical College, Narhe, Pune-411041(Maharashtra)., Ingole Sonali ,   Associate Professor, Department of Obstetrics & Gynecology, Smt. Kashibai Navale Medical College, Narhe, Pune-411041(Maharashtra)., Sintre Milind ,   Resident, Department of Obstetrics & Gynecology, Smt. Kashibai Navale Medical College, Narhe, Pune-411041(Maharashtra).
Published: June 2022

Background and objective:The technological advancement in endoscopic instruments has revolutionized the management of female infertility. Our objective was to compare the diagnostic and therapeutic efficacy of Hysterolaparoscopy over Hysterosalpingography in infertile patients and also to assess is there any needfor doing HSG ininfertility evaluation. Methods:100 infertile women who wereconfirmed to have normal ovulatory cycles, hormonal assays, and husband semen analysis were enrolled.HSG was performed as a basic test for the evaluation of tubes and the uterine cavity. All 100 women were also subjected to combine laparoscopic and hysteroscopy, and comparative evaluation done. Results:A total of 100 subjects were analyzed in this study. 81% of subjects had primary infertility and 19% had secondary infertility. 89%of patients had normal uterine cavity on HSG whereas 19 % patients had abnormal findings on Hysteroscopy. Hysteroscopy operative procedureswere done among 21% cases.In our study, 40 % patients had abnormal findings on laparoscopy, and corrective surgeries were performed in 23 % patients in the same setting. Laparoscopic operative procedures involved adhesiolysis in 7.0% of cases, PCOD ovarian drilling in 6.0% of patients, myomectomy in 2.0% of cases, Tubal reconstructive surgery in 2.0% of cases, ovarian cystectomy in2.0%of cases and ablation for endometriosis was done in eight (8.0%) cases. Conclusion: See and treat the infertility factors shall be the reality and future argument in modern Gynecology within the limitations of this study. We conclude that hysterolaparoscopy should be offered as the first- line gold standard modality for the evaluation of infertility wherever the procedure is available.
CARACTERISATION PHENOTYPIQUE QUALITATIVE ET IDENTIFICATION DES RONIERS (BORASSUS SPP.) DES REGIONS DE LAGNEBY-TIASSA, DU BELIER, DU HAUT-SASSANDRA, DE LA MARAHOUE ET DU NZI EN COTE DIVOIRE
Authors:   Laboratoire damelioration De La Production Agricole, UFR Agroforesterie, Universite Jean Lorougnon Guede, Daloa, Cote dIvoire., Moroh Joseph Akaza , Konan Severin Mlan ,   Departement de Sociologie - Anthropologie, Universite Jean Lorougnon Guede, Daloa, Cote dIvoire., Doffou Selastique Akaffou ,   Laboratoire damelioration De La Production Agricole, UFR Agroforesterie, Universite Jean Lorougnon Guede, Daloa, Cote dIvoire., Bosson Romain Gnamou ,   Laboratoire damelioration De La Production Agricole, UFR Agroforesterie, Universite Jean Lorougnon Guede, Daloa, Cote dIvoire.
Published: June 2022

The distributions of Borassus aethiopum and Borassus akeassii, observed in certain ecological zones, do not overlap. Also, characterization by qualitative parameters provides useful informations on palmyra stands. But in Cote dIvoire, the qualitative characteristics and the distribution of palmyra are not known. Thus, this investigation aimed to describe the phenotypic diversity of the palmyra of the regions of Agneby-Tiassa, Belier, Haut-Sassandra, Marahoue and Nzi, identify them and establish their distributions. To do this, we collected qualitative data on palmyra in various stands in these regions and then processed them. At the end of this, we observed three, six, two, four and two colorations, respectively, of the trunk, the petiole, the immature fruit, the ripe fruit, the pulp of ripe fruits, and described three forms of fruit. In addition, the palmyra of Marahoue present more variable qualitative characteristics. The results indicate that the palmyra of the regions of Nzi, Belier and Agneby-Tiassa belong to Borassus aethiopum and those of Haut-Sassandra to Borassus akeassii. In Marahoue, palmyra of both species are encountered, even already from Zambakro. This study showed that the palmyra present variable modalities for each of the six qualitative parameters considered. These made it possible to highlight a distribution zone specific to each species and a transition zone. These informations should be used for other studies on palmyra populations in Cote dIvoire.
EFFECT OF BIOGAS SLURRY ON COMPOSTING PROCESS AND QUALITY OF CATTLE MANURE-BASED COMPOST AND VERMICOMPOST
Authors:   Department of Chemistry, University of Rajshahi, Rajshahi-6205, Bangladesh., Apel Akteruzzaman , Nazmul Islam Md. ,   Department of Chemistry, University of Rajshahi, Rajshahi-6205, Bangladesh., Shahidul Islam Md. ,   Department of Chemistry, University of Rajshahi, Rajshahi-6205, Bangladesh., Ashraful Hoque Md. ,   Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh., Nurul Islam Md. ,   Department of Chemistry, University of Rajshahi, Rajshahi-6205, Bangladesh.
Published: June 2022

Regarding the increasing demand of good organic manure, we made an attempt to generate a modified vermicompost from biogas slurry amended cattle manure. For this, six trials including control were run in pots by adding 2âˆ’10% (w/w, fw.) biogas slurry to cattle manure for 21 days for composting and 60 days for vermicomposting using E. eugeniae earthworms (10 per 500 g) under aerobic condition keeping moisture level of 70Â±5% within the overall temperature range of 13âˆ’39Â°C. The use of bacterial inoculants increased the agricultural quality of the compost and accelerated the composting process. After being maturity, pH of the vermicompost reached to 7.66, indicating that it has excellent nutrient releasing capability. Since EC of the produced vermicompost is less than 1.60 dS mâˆ’1, it has high cation-exchange capacity, which is beneficial to soil texture and plant health. An increment in 163% of available P in the vermicompost (6.35 Â± 0.78 mg g-1, dw) than that in the compost and the highest increment of 264% with respect to control indicated that it was a better organic fertilizer and hence might be an alternative to P2O5. The worm-processed material for trials T1-T5 showed 10.0â€“32.5% more exchangeable K than control (T0). An increment of 43.84% in total N and an decrease of 15.6% in total organic C makes the vermicompost ideal for use for its lowest C:N ratio of 15:1, whereas it was 35:1 in compost. Each of the N-P-K contents was elevated significantly that made it a potential organic fertilizer. Comparison of the key parameters with vermicomposts of buffalo, pig, goat, horse, chicken and sheep manures of different countries confirms that the produced vermicompost could serve as an excellent organic manure.
Recombinant Expression, Purification and PEGylation of DNA Ligases
Authors: Zuma Lindiwe Khumbuzile , Gasa Nothando Lovedale , Mazibuko Xolani , Simelane Mthokozisi Blessing C. , Pillay Priyen , Kwezi Lusisizwe , Tsekoa Tsepo , Pooe Ofentse Jacob
Published: June 2022

Background:

Reagent proteins such as DNA ligases play a central role in the global
reagents market. DNA ligases are commonly used and are vital in academic and science research
environments. Their major functions include sealing nicks by linking the 5’-phosphorylated end to a
3’-hydroxyl end on the phosphodiester backbone of DNA, utilizing ATP or NADP molecules as an
energy source.

Objective:

The current study sought to investigate the role of PEGylation on the biological activity
of purified recombinant DNA ligases.

Method:

We produced two recombinant DNA ligases (Ligsv081 and LigpET30) using E. coli
expression system and subsequently purified using affinity chromatography. The produced proteins
wereconjugated to site specific PEGylation or non-specific PEGylation. FTIR and UV-VIS
spectroscopy were used to analyze secondary structures of the PEG conjugated DNA ligases.
Differential scanning fluorimetry was employed to assess the protein stability when subjected to
various PEGylation conditions.

Results:

In this study, both recombinant DNA ligases were successfully expressed and purified as
homogenous proteins. Protein PEGylation enhanced ligation activity, increased transformation
efficiency by 2-foldfor plasmid ligations and reduced the formation of protein aggregates.

Conclusion:

Taken together, site-specific PEGylation can potentially be explored to enhance the
biological activity and stability of reagent proteins such as ligases.
Discovery of Polo-like Kinase 4 Inhibitors for the Treatment of Cancer:
A Mini Patent Review
Authors: Xie Zhouling , Liao Chenzhong , Shu Yang , Liu Yajing , Bian Shirong
Published: June 2022

Polo-like kinase 4 (PLK4), a serine/threonine kinase, is a member of the PLK family.
As a key regulator of the cell cycle, PLK4 controls centrosome duplication and mitosis. Abnormal
PLK4’s function can induce centrosome amplification, leading to tumorigenesis, therefore, PLK4
has been regarded as a promising target for cancer therapy, and PLK4 inhibitors have potentials to
treat multiple cancers and other PLK4-associated human disorders, such as myelodysplastic syndrome.
In addition, PLK4 may function as a DNA-damage sensitizer, therefore improving the
efficacy of chemotherapy. To date, some small-molecule inhibitors with different chemical scaffolds
targeting PLK4 have been reported, among which, CFI-400945 has entered clinical trials for
the treatment of various solid tumors, myeloid leukemia, and myelodysplastic syndrome. In this
review, the structure and biological functions of PLK4 with other homologous PLKs are compared;
the roles of PLK4 in different cancers are reviewed; and PLK4 inhibitors disclosed in patent
or literature are summarized. Used alone or in combination with other anticancer drugs in
preclinical and clinical studies, PLK4 inhibitors have shown significant efficacy in the treatment
of different cancers, demonstrating that PLK4 could be a critical target for cancer diagnosis and
therapy. However, our understanding of PLK4 is still limited, and novel mechanisms of PLK4
should be identified in future studies.
Recent Update on the Alzheimer's Disease Progression, Diagnosis and
Treatment Approaches
Authors: Kesharwani Prashant , Malaiya Akanksha , Singhai Mansha , Singh Manisha , Prajapati Shiv Kumar , Choudhury Hira , Fatima Mahak , Alexander Amit , Dubey Sunil Kumar , Greish Khaled
Published: June 2022

Alzheimer's disease (AD) is a multifactorial, progressive, neurodegenerative disorder, manifested by the loss of memory and cognitive abilities, behavioral disturbance and progressive impairment of activities of daily life. The sharp rise in the number of AD patients has brought it with-in the top eight health issues in the world. It is associated with the distribution of misfolded aggre-gates of protein within the brain. However, Alois Alzheimer initially mentioned that the reduction in brain volume in AD might be associated with the “deposition of a special substance in the cortex”. The resulting plaque found in extracellular space in the AD brain and hippocampus region, known as senile plaques, is the characteristic feature underlying Alzheimer’s pathology, where the role of amy-loid-β (Aβ) peptide formation from proteolytic cleavage of amyloid precursor protein (APP) by secre-tase enzyme is eminent. Therefore, this review has highlighted the molecular pathophysiology of AD with a variety of available diagnostic and treatment strategies for the management of the disease, with a focus on the advancement toward clinical research to provide new effective and safe tool in the diagnosis, treatment or management of AD.
Structural Perspective of Benzophenones Targeting Tubulin as Anticancer Agents
Authors: Kashaw Sushil Kumar , Chourasia Prerna , Asati Vivek , Agarwal Shivangi , Kashaw Varsha , Das Ratnesh
Published: June 2022

Cancer is the main cause of death and the most significant determinant of life expectancy in every country in the twenty-first century. According to the World Health Organization (WHO) cancer is responsible for major cause of death globally. Benzophenone derivatives are found in a variety of naturally occurring compounds which are known to be pharmacologically efficacious against a variety of diseases, including cancer. Microtubules are thought to be a good target for cancer chemotherapies. Microtubule polymerization and depolymerization are induced by a variety of natural, synthetic, and semisynthetic chemicals having a benzophenone nucleus, affecting tubulin dynamics. Several medications that affect microtubule dynamics are in various stages of clinical trials, including Combretastatins (phase II), Vincristine (clinically approved), Paclitaxel (in clinical usage), and epothilone (phase III), and only a few have been patented. Benzophenone derivatives act by targeting the colchicine binding site of microtubules damage them and cause cell cycle arrest in the G2-M phase. Belonging to this class of molecules, phenstatin, a potent inhibitor of tubulin polymerization, shown strongly inhibited cancer cell growth and arrest the G2/M phase of the cell cycle by targeting the colchicine binding site of microtubules. In the present manuscript we described the benzophenone as tubulin polymerization inhibitors their structure activity relationships (SARs) and molecular docking studies that reveal its binding affinity with the colchicine binding site.
Ethyl Pyruvate Alleviating Inflammatory Response after Diabetic Cerebral
Hemorrhage
Authors: Song Yuejia , Qi Jiping , Wang Yueying , Li Ke , Liu Zhiyi , Sun Yulan , Wang JiaJun , Liu Qi
Published: June 2022

Objective:

This study’s purpose is to investigate the neuroprotective role of ethyl pyruvate
(EP) in the pathogenesis of diabetic intracerebral hemorrhage.

Methods:

The present study used a mouse model of collagenase-induced intracerebral hemorrhage
(ICH) and streptozotocin-induced diabetes. The C57BL/6 mice were randomly divided into 3
groups: sham operation, diabetic cerebral hemorrhage, and diabetic cerebral hemorrhage with EP.
The EP (80 mg/kg) and EP (50 mg/kg) were injected intraperitoneally one day and one hour before
modeling. The protein expression levels of high mobility group box 1 (HMGB1) and NOD-like
receptors 3 (NLRP3) were detected with western blot. The mRNA levels of HMGB1 and toll-like
receptor 4 (TLR4) were measured by quantitative real-time polymerase chain reaction (PCR). Immunofluorescence
and ELISA were performed to confirm some inflammatory factors.

Results:

Compared to the normal diabetic intracerebral hemorrhage group, the mRNA and protein
expression levels of HMGB1 and TLR4 were downregulated in the EP-affected group with diabetic
cerebral hemorrhage, together with the downregulation of the expression of inflammasomes, including
NLRP3, apoptosis-associated speck-like protein containing CARD (ASC), and caspase 1.

Conclusions:

EP can reduce the inflammatory response after diabetic intracerebral hemorrhage and
may inhibit the activation of inflammasomes by the HMGB1/TLR4 pathway.
Antioxidant Activity and Inhibitory Effect of Curcumin on Some Enzymes
Involved in Several Diseases: Acetylcholinesterase, Butyrylcholinesterase,
α-glucosidase and Tyrosinase
Authors: Sobhi Widad , Bisset Seghira , Bensouici Chawki , khenchouche Abdelhalim
Published: June 2022

Aim:

This study investigates the potential effect of pure curcumin on the inhibition of different
enzymes involved in several diseases.

Background:

Several chronic diseases such as Alzheimer’s, diabetes, and Parkinson’s are related to oxidative
stress and enzyme activity. Today, various plant origin products are beneficial against several
chronic diseases with secondary metabolites such as phenolic compounds. Curcumin, a polyphenol yellow-
orange pigment in turmeric spices, has a wide range of biological activities with quite a safety.

Objective:

This study was aimed to investigate the antioxidant and inhibitory potential against key enzymes involved in human pathology, namely Alzheimer's disease (Acetylcholinesterase (AChE), and Butyrylcholinesterase (BChE)), diabetes (α-glucosidase) and hyperpigmentation and Parkinson’s diseases (Tyrosinase) of curcumin.

Methods:

1,1-diphenyl-2-picrylhydrazyl free radical (DPPH●) and hydrogen peroxide radicals (H2O2)
assays were used to evaluate the antioxidant capacity of curcumin, and enzyme inhibitory activity was
evaluated using in vitro standard procedures.

Results:

Curcumin exhibited an excellent antioxidant effect with an IC50 value significantly less than
Vit C reference. In enzyme inhibitory activity, curcumin demonstrated excellent inhibitory activity
against AChE, BChE and α-glucosidase. The finding showed that curcumin was significantly less than
the reference galantamine against AChE but more than the references galantamine and acarbose against
BChE and α-glucosidase, respectively. Whereas for anti-tyrosinase activity, curcumin displayed weak
inhibitory activity compared with the standard inhibitor, Kojic acid.

Conclusion:

These results indicated that curcumin showed promising antioxidant, anti-Alzheimer, and
anti-diabetic properties and might be used as potential natural drugs against these diseases.
FAMILIA GRAMINEAE : SUBFAMILIA ARISTIDOIDEAE
Authors:   Departamento de Botánica, Universidad Autónoma Agraria Antonio Narro, Valdés Reyna Jesús , Allred Kelly W. ,   Department of Animal & Range Sciences, New Mexico State University
Published: June 2022

La subfamilia Aristidoideae incluye solamente una tribu, las Aristideae, que hasta hace relativamente poco junto con las tribus Danthonieae y Arundineae se consideraba por varios taxónomos como parte de la subfamilia Arundinoideae, pero ahora se reconoce que la interpretación amplia de las Arundinoideae no es satisfactoria, pues abarca un grupo polifilético. La tribu consta de un solo género.
FAMILIA LENTIBULARIACEAE
Authors:   Instituto de Ecología, A.C., Centro Regional del Bajío, Zamudio Ruiz Sergio
Published: June 2022

Familia de plantas insectívoras, de amplia distribución mundial, con tres géneros y más de 300 especies. En la región de estudio sólo se presentan dos géneros.
FAMILIA URTICACEAE
Authors:   Instituto de Ecología, A.C., Centro Regional del Bajío, Steinmann Victor W.
Published: June 2022

La familia cuenta con alrededor de 45 géneros y 1000 especies distribuidas en los trópicos y subtrópicos de todo el mundo, pero concentradas en el sureste de Asia. Unas pocas se encuentran en regiones templadas de ambos hemisferios. Por lo general viven en sitios húmedos y en México llegan a su mayor diversidad en el bosque mesófilo de montaña. En México Urticaceae se manifiesta con 18 géneros (uno de los cuales, Soleirolia, es introducido) y alrededor de 90 especies. Se registran 11 géneros y 26 especies en el área de estudio.
FAMILIA LOGANIACEAE
Authors:   Universidad Nacional Autónoma de México, Facultad de Ciencias, Laboratorio de Plantas Vasculares, Islas Hernández C. Sofia , Alvarado-Cárdenas Leonardo O. ,   Universidad Nacional Autónoma de México, Facultad de Ciencias, Laboratorio de Plantas Vasculares
Published: June 2022

Familia distribuida en las regiones tropicales y subtropicales del Viejo y Nuevo Mundo, con 13 géneros y cerca de 400 especies en su nueva circunscripción. En la región de estudio se encuentra distribuido solamente el género Spigelia.
FAMILIA EQUISETACEAE
Authors:   Instituto de Ecología, A.C., Centro Regional del Bajío, Tejero-Díez J. Daniel ,   Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Iztacala
Published: June 2022

Familia de un género con 15 especies distribuidas alrededor del mundo. En México y en otros países, las especies de Equisetum son ampliamente usadas como medicinales; en los mercados mexicanos se les conoce como cola de caballo (nombre de origen europeo) o carricillo.
FAMILIA MYRTACEAE
Authors:   Instituto de Ecología, A.C., Sánchez-Chávez Erandi , Zamudio Sergio ,   Instituto de Ecología, A.C., Centro Regional del Bajío
Published: June 2022

Familia de distribución tropical, con 140 géneros y ca. 7000 especies a nivel mundial. En México se registran 10 géneros y aproximadamente 113 especies; en el Bajío y regiones adyacentes se encuentran cuatro géneros y 14 especies nativas más una variedad; no se incluyen Eucalyptus camaldulensis, E. globulus, E. robusta, Callistemon citrinus, C. rigidus y Syzygium sp., que se cultivan comúnmente en la región.