bluuebunny/crossref_metadata_2025 huggingface dataset:
Staying safe, feeling welcome, being seen: How spatio‐temporal configurations affect relations of care at an inclusive health and wellness centre
Authors: Plage Stefanie , Baker Kirsten , Parsell Cameron , Stambe Rose‐Marie , Kuskoff Ella , Mansuri Arif
Published: September 2023

Background

People experiencing homelessness also experience poorer health and frequently attend acute care settings when primary health care would be better equipped to meet their needs. Existing scholarship identifies a complex mix of individual and structural‐level factors affecting primary health care engagement driving this pattern of health services utilisation. We build on this existing knowledge, by bringing the spatio‐temporal configurations of primary health care into focus. Specifically, we interrogate how space and time inflect situated practices and relations of care.

Methods

This study took an ethnographic approach and was conducted 2021–2022 at an inclusive health and wellness centre ("the Centre") in Southeast Queensland, Australia. The data consists of 46 interviews with 48 people with lived experience of homelessness, including participants who use the services offered at the Centre (n = 26) and participants who do not (n = 19). We also interviewed 20 clinical and non‐clinical service providers affiliated with the Centre and observed how service delivery took place. Interviews and observations were complemented by visual data, including participant‐produced photography. All data were analysed employing a narrative framework.

Results

We present three interrelated themes demonstrating how space and time affect care, that is ‘staying safe’, ‘feeling welcome’ and ‘being seen’. ‘Staying safe’ captures the perceptions and practices around safety, which sit in tension with making service users feel welcome. ‘Feeling welcome’ attends to the sense of being invited to use services free of judgment. ‘Being seen’ depicts capacities to see a health care provider as well as being understood in one's lived experience.

Conclusion

Spatio‐temporal configurations, such as attendance policies, consultation modalities and time allocated to care encounters afford differential opportunities to nurture reciprocal relations. We conclude that flexible service configurations can leverage a relational model of care.

Patient or Public Contribution

Service providers were consulted during the design stage of the project and had opportunities to inform data collection instruments. Two service providers contributed to the manuscript as co‐authors. People with lived experience of homelessness who use the services at the inclusive health centre contributed as research participants and provided input into the dissemination of findings. The photography they produced has been featured in an in‐person exhibition, to which some have contributed as consultants or curators. It is hoped that their insights into experiences of welcomeness, safety and being seen will inform flexible and relational primary health care design, delivery, and evaluation to better cater for people experiencing housing instability and poverty.
Preparation of spherical AlON powder and high transparent ceramics by pressureless sintering
Authors: Zhang Yiming , He Qing , Wu Haoyang , Zhang Zhirui , Jia Baorui , Lu Huifeng , Gu Siyong , Li Tao , Guo Dengshuai , Zhu Zaiwen , Hou Dong , Qu Xuanhui , Qin Mingli
Published: September 2023

The sphericity and fluidity of the powder play a crucial role in the forming and sintering of ceramics. High‐performance AlON spherical powder was prepared for the first time by spray drying of powder fired by solid‐phase reaction, and the preparation of AlON transparent ceramics was realized under pressureless sintering conditions. The effect of AlN dosage on the purity of AlON powder was investigated, and the powder fluidity was improved by spray drying technique. Then the best spray drying process was selected by scanning electron microscopy, AD/TD, green density, MIP and transmittance measurements. The AlON particles obtained under the optimal granulation process have good dispersibility, high green density after pressing, and uniform pore distribution. After dry pressing and sintering at 1900°C for 20 h, the AlON ceramics with linear transmissivity of 84.3% at 2 μm wavelength can be obtained. The excellent pressing and sintering properties of AlON granulated particles make it possible for large‐scale production.
A comparative study of cerium oxides formed by pulsed laser ablation and continuous wave laser heating
Authors: Auner Alexander W. , Burton Mark A. , Nagel Lauren A. , Crowhurst Jonathan C. , Weisz David G. , Knight Kimberly B.
Published: September 2023

The formation of Ce2O3 from cerium metal requires complex reducing conditions. Alternatively, we show that different stoichiometries and structures of cerium oxides can be generated in high‐temperature laser experiments by varying the availability of oxygen and the use of either pulsed laser ablation (PLA) or continuous wave (CW) laser heating of cerium metal. X‐ray diffraction and Raman spectroscopy suggest that a low structural order Ce2O3/CeO2 mixed structure is formed in a 1% O2 atmosphere with PLA. For CW heating in a 1% O2 environment, Raman measurements show the formation of crystalline A‐type Ce2O3 in proximity to the point of laser impingement. These experiments were repeated in 18O2 and allowed us to report for the first time the corresponding Raman spectrum of Ce218O3.
The impact of parent mediation on young children's home digital literacy practices and learning: A narrative review
Authors: Soyoof Ali , Reynolds Barry Lee , Neumann Michelle , Scull Janet , Tour Ekaterina , McLay Katherine
Published: September 2023

Background

In today's society, a growing body of literature attests to the importance of young children's early digital literacy skills in their home environments and how acquisition of these digital literacy skills relates to their future learning and digital literacy.

Objectives

Research on young children's digital literacy practices at home was reviewed to explore the positive and negative influences on early learning. This is important due to the children's rapid uptake of online digital technologies over the past decade.

Methods

Peer‐reviewed research articles on home digital literacy practices of children (aged 0–8 years old) published between 2010 and 2021 from four education databases were carefully selected based upon pre‐determined criteria and examined using content analysis.

Results and Conclusion

A high proportion of studies (29 of the 31; 93.5%) demonstrated significant benefits of young children gaining a range of skills, including digital operational, early literacy and language, socio‐emotional, and STEM, through the use of digital technologies at home. Five of the 31 (16.12%) studies reported negative effects of digital technologies in the home context, including distraction, aggressive behaviour, and false self‐confidence. Tablets and smartphone use gained greater momentum in the home context, especially between 2015 and 2021, and there was a positive shift in parental mediation, family involvement, and the children's home digital literacy practices.

Implications

By leveraging children's acquisition of digital literacy skills in the home and taking into account the sociocultural context, we can enhance young children's preparation for the future and provide opportunities for skill development across various learning domains.
The glucose metabolic reprogramming in hepatitis B virus infection and hepatitis B virus associated diseases
Authors: Wang Hangle , Zhang Jun
Published: September 2023

Hepatitis B virus (HBV) infection is closely related to viral hepatitis, liver cirrhosis, and hepatocellular carcinoma. HBV infection can reprogram metabolism processes of the host cells including glucose metabolism. The aberrant glucose metabolism may aid in viral infection and immune escape and may contribute to liver associated pathology. In this review, we discussed the interplay between HBV infection and glucose metabolism, which may provide new insights into HBV infection and pathology, novel intervention targets for HBV‐related diseases.
Reply to: Artificial intelligence in geriatric medicine: Potentials and pitfalls
Authors: Haque Nihal
Published: September 2023

This letter comments on the letter by Richard G. Stefanacci in this issue.
Artificial intelligence in geriatric medicine: Potential and pitfalls
Authors: Stefanacci Richard G.
Published: September 2023

See the Reply by Nihal Haque in this issue.
Agrarian change through speculation: Rural elites as land brokers for mining in Colombia
Authors: Dietz Kristina
Published: September 2023

This paper studies the connections between the expansion of mining capital, speculative forms of land grabbing and agrarian transformation. It is argued that in periods of commodity boom, the landowning rural elite benefits from mining through speculative land deals with mining companies. They act as ‘land brokers’ for the mining firms, helping them to overcome a significant barrier to land accumulation through the de facto abolition of landed property. The analysis is based on a qualitative case study on the expansion of coal mining in central Cesar in northern Colombia. To develop my arguments, I refer to the concept of accumulation by dispossession as defined by Michael Levien, and historical materialist approaches on rent, and speculative land dispossession. In addition, I use concepts developed for studying coercive land grabbing and agrarian elite participation in armed conflicts to analyse the mechanisms applied to (coercively) acquire rights to land. It is concluded that with high global prices for minerals, metals and fossil fuels, the expansion of mining in the countryside fosters a process of agrarian change through land speculation that is articulated in a reconcentration of landed property, a re‐strengthening of the rural landowning elite and the dissolution of peasant agriculture.
Work engagement, psychological empowerment and relational coordination in long‐term care: A mixed‐method examination of nurses' perceptions and experiences
Authors: Rawson Helen , Davies Sarah , Ockerby Cherene , Pipson Ruby , Peters Ruth , Manias Elizabeth , Redley Bernice
Published: September 2023

Nurse engagement, empowerment and strong relationships among staff, residents and families, are essential to attract and retain a suitably qualified and skilled nursing workforce for safe, quality care. There is, however, limited research that explores engagement, empowerment and relational coordination in long‐term care (LTC). Nurses from an older persons’ mental health and dementia LTC unit in Australia participated in this study. Forty‐one nurses completed a survey measuring psychological empowerment, work engagement and relational coordination. Twenty‐nine nurses participated in individual interviews to further explore these concepts. Although nurses reported high psychological empowerment and work engagement, their relationships with key stakeholders varied. Our findings suggest that nurses in LTC require both supports and opportunities to contribute as active members of the multiprofessional care team that includes tailored education, professional development and positive interactions within the care team. Regular support is needed to enable nurses to feel empowered, foster relationships and communication, and facilitate work engagement. Based on these findings, we suggest that it is important to find ways to ensure that all who provide care perceive that they are part of the whole care team and able to contribute to the care and well‐being of people in LTC.
MsWRKY33 increases alfalfa (Medicago sativa L.) salt stress tolerance through altering the ROS scavenger via activating MsERF5 transcription
Authors: Ma Lin , Li Xin , Zhang Jinjin , Yi Dengxia , Li Feng , Wen Hongyu , Liu Wenhui , Wang Xuemin
Published: September 2023

Alfalfa (Medicago sativa L.) is considered to be the most important forage crop on a global scale. Nevertheless, soil salinity significantly decreases productivity, seriously threatening food security worldwide. One viable strategy is to explore salt stress‐responsive factors and elucidate their underlying molecular mechanism, and utilize them in further alfalfa breeding. In the present study, we designated MsWRKY33 as a representative salt stress‐responsive factor preferentially expressed in alfalfa roots and leaves. Subsequently, it was demonstrated that MsWRKY33 was localized in the cell nucleus, and functioned as a transcriptional activator of the W‐box element. Transgenic alfalfa overexpressing MsWRKY33 displayed enhanced salt stress tolerance and antioxidant activities with no significant difference in other agronomic traits. Transcriptome profiling of MsWRKY33 transgenic alfalfa under control and salt treatment unveiled significantly altered expression of reactive oxygen species (ROS) scavenger genes in transgenic alfalfa. Subsequent examination revealed that MsWRKY33 binded to the promoter of MsERF5, activating its expression and consequently fine‐tuning the ROS‐scavenging enzyme activity. Furthermore, MsWRKY33 interacted with the functional fragment of MsCaMBP25, which participates in Ca2+ signaling transduction. Collectively, this research offers new insight into the molecular mechanism of alfalfa salt stress tolerance and highlights the potential utility of MsWRKY33 in alfalfa breeding.
Industry selection of regional migrants in China: The role of urban industry structures of home and destination
Authors: Ma Xiangjun , Qiu Larry , Son Hyelim
Published: September 2023

Regional migrants constitute a large share of the labour market in China and therefore have important implications for welfare, resource reallocation and economic development. However, few works exist in studying how regional migrants select industries in destination cities. This study examines the role of urban industry structures of migrants' origin and destination locations in determining the industry selection of regional migrant workers in China. We apply a variant of the Roy model to guide the empirical analysis and generate the following predictions: the industry selection of a cross‐province migrant is negatively affected by the industry strength of her home province and positively affected by that of her destination. Using data from a nationwide migrant survey in China, we verify the patterns of migrants' industry selection consistent with the predictions of the model. We also find that regional migration is more likely to occur between the origin and destination with more dissimilar industry structures.
Extracellular heat shock protein 70 levels in tumour‐bearing dogs and cats treated with radiation therapy and hyperthermia
Authors: Nytko K. J. , Weyland M. S. , Dressel‐Böhm S. , Scheidegger S. , Salvermoser L. , Werner C. , Stangl S. , Carpinteiro A. C. , Alkotub B. , Multhoff G. , Bodis S. , Rohrer Bley C.
Published: September 2023

Hyperthermia is a form of a cancer treatment which is frequently applied in combination with radiotherapy (RT) to improve therapy responses and radiosensitivity. The mode of action of hyperthermia is multifactorial; the one hand by altering the amount of the blood circulation in the treated tissue, on the other hand by modulating molecular pathways involved in cell survival processes and immunogenic interactions. One of the most dominant proteins induced by hyperthermia is the major stress‐inducible heat shock protein 70 (Hsp70). Hsp70 can be found in the blood either as a free‐protein (free HSP70) derived from necrotic cells, or lipid‐bound (liposomal Hsp70) when it is actively released in extracellular vesicles (EVs) by living cells. The aim of the study was to evaluate the levels of free and liposomal Hsp70 before and after treatment with RT alone or hyperthermia combined with radiotherapy (HTRT) in dogs and cats to evaluate therapy responses. Peripheral blood was collected from feline and canine patients before and at 2, 4, 6 and 24 h after treatment with RT or HTRT. Hsp70 enzyme‐linked immunosorbent assays (ELISAs) were performed to determine the free and liposomal Hsp70 concentrations in the serum. The levels were analysed after the first fraction of radiation to study immediate effects and after all applied fractions to study cumulative effects. The levels of free and liposomal Hsp70 levels in the circulation were not affected by the first singular treatment and cumulative effects of RT in cats however, after finalizing all treatment cycles with HTRT free and liposomal Hsp70 levels significantly increased. In dogs, HTRT, but not treatment with RT alone, significantly affected liposomal Hsp70 levels during the first fraction. Free Hsp70 levels were significantly increased after RT, but not HTRT, during the first fraction in dogs. In dogs, on the other hand, RT alone resulted in a significant increase in liposomal Hsp70, but HTRT did not significantly affect the liposomal Hsp70 when cumulative effects were analysed. Free Hsp70 was significantly induced in dogs after both, RT and HTRT when cumulative effects were analysed. RT and HTRT treatments differentially affect the levels of free and liposomal Hsp70 in dogs and cats. Both forms of Hsp70 could potentially be further investigated as potential liquid biopsy markers to study responses to RT and HTRT treatment in companion animals.
A non‐adaptationist hypothesis of play behaviour
Authors: Lewis Amelia
Published: September 2023

Play is a suite of apparently non‐functional, pleasurable behaviours observed in human and non‐human animals. Although the phenomenon has been studied extensively, no adaptationist behavioural theory of how play evolved can be supported by the available evidence. However, the advancement of the extended evolutionary synthesis and developments in systems biology offer alternative avenues for non‐adaptationist physiological hypotheses. I therefore propose a hypothesis of play, based upon a complex ACh activity that is under agential control of the organism, whereby play initiates ACh‐mediated feedforward and feedback processes which act to: (i) regulate metabolic processes; (ii) form new ACh receptors via ACh mRNA activity; (iii) mediate attention, memory consolidation and learning; and (iv) mediate social behaviours, reproduction and embryonic development. However, play occurs across taxa, but does not occur across all taxonomic groups or within all species of a taxonomic group. Thus, to support the validity of the proposed hypothesis, I further propose potential explanations for this anomaly, which include sampling and observer biases, altricial versus precocial juvenile development, and the influence of habitat niche and environmental conditions on behaviour. The proposed hypothesis thus offers new avenues for study in both the biological and social sciences, in addition to having potential applications in applied sciences, such as animal welfare and biomedical research. Crucially, it is hoped that this hypothesis will promote further study of a valid and behaviourally significant, yet currently enigmatic, biological phenomenon.

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Experimental Force Coefficients of a Hydrostatic Thrust Bearing and Comparisons to Predictions
Authors: Lim Homin , Ryu Keun
Published: September 2023

Reliable fluid film bearings for high-performance turbomachinery provide significantly extended operating life with controllable stiffness and damping force coefficients at low cost. Hydrostatic thrust bearings support thrust loads and control axial motions. This paper reports measurements and predictions of static and dynamic load characteristics of a hydrostatic thrust bearing fed with pressurized air. The current bearing model uses dynamic axial periodic motion amplitudes, that is, models as a function of the initial film thickness, to predict the dynamic force coefficients. Note that, presently, the recorded frequencies and their amplitudes of pneumatic hammer instability along the axial direction are implemented as an external excitation source. The measured and predicted static stiffnesses increase as the film thickness decrease and supply pressure into the bearing increase. The predicted dynamic force coefficients show a favorable agreement with the test data. Analysis reveals the negative damping coefficients and damping ratios as a distinctive onset condition of the present pneumatic hammer instability of the test bearing. A careful recess design and adequate operation conditions along with an accurate prediction can avoid or prevent undesirable pneumatic hammer instability in hydrostatic thrust bearings. This work extends the knowledge database of hydrostatic thrust bearings with pneumatic hammer instability and provides their design guideline for various operating conditions.
Dynamic Forced Performance of an O-Rings Sealed Squeeze Film Damper Lubricated With a Low Supply Pressure and a Simple Method to Quantify Air Ingestion
Authors: Rodríguez Bryan , San Andrés Luis
Published: September 2023

Contemporary squeeze film dampers (SFDs) in air-breathing engines are short in length to limit weight and part count and lubricated with a low feed pressure to reduce oil storage and pumping power. In SFDs, O-rings (ORs) restrict side leakage and increase the viscous damping while a adding a modest centering stiffness. Continuing a long-term project characterizing SFDs for aircraft engines and extending the original work (San Andrés and Rodríguez, 2021, “On the Experimental Dynamic Force Performance of a Squeeze Film Damper Supplied Through a Check Valve and Sealed With O-Rings,” ASME J. Eng. Gas Turb. Power, 143(11), p. 111011) the paper details measurements of the forced performance of an OR sealed damper (OR-SFD) with diameter D = 127 mm, land length L = 0.2 D, and radial clearance c = 0.0022 D. Lubricant ISO VG 2 supplied at 0.69 bar(g) fills an upstream oil plenum and flows into the middle of the land through a single orifice configured with a check valve. Measurements of applied single-frequency dynamic loads, along with the ensuing damper displacements and accelerations serve to identify the parameters of the test structure, ORs, and SFD. The tests encompass centered whirl motions with amplitude r = 0.05–0.45c, and a range of whirl frequencies, ω = 10–130 Hz. Note the squeeze velocity vs =  rω reaches 102 mm/s. The ORs force coefficients are nearly invariant with frequency but do depend on the orbit amplitude. The ORs' stiffness (KOR) decreases by 75% as the motion amplitude increases, r → 0.45c, likely due to the large elastic deformations and slow recovery of the ORs material. For small amplitude motions (r/c = 0.05 and 0.10), the ORs damping coefficient (COR) is ∼10% of the overall coefficient for the lubricated system (CL), while for r/c > 0.25, COR ∼ 0.03CL. For small amplitudes of whirl (r ≤ 0.25c), the SFD experimental viscous damping (CSFD) and added mass (MSFD) coefficients, identified over a shorter frequency range (vs<30 mm/s) equal theoretical magnitudes for a fully sealed damper. As the orbit size grows to r = 0.45c MSFD drops nearly by 75% and CSFD decreases by ∼40% due to the onset of both lubricant cavitation and air ingestion occurring for squeeze velocities vs ≥ 24.5 mm/s, as also seen in the recorded dynamic pressures and video recordings of a bubbly mixture leaving the damper. A comprehensive flow model predicts CSFD and MSFD about 8% and 12% larger than the experimentally identified coefficients. A novel approach enables the estimation of the gas volume fraction (GVF) generated in the damper which rapidly increases as vs grows. The simple procedure draws into a deflated balloon the material contents in the squeeze film, weighs the sample, and identifies its volume to produce an estimate of the GVF. The procedure to quantify the GFV will assist in the validation of predictive tools.
Opsonization-independent antigen-specific recognition by myeloid phagocytes expressing monoclonal antibodies
Authors: Neumaier Michael , Giesler Sophie , Ast Volker , Roemer Mathis , Voß Timo-Daniel , Reinz Eileen , Costina Victor , Schmelz Martin , Nürnberg Elina , Nittka Stefanie , Leppä Aino-Maija , Rudolf Ruediger , Trumpp Andreas , Fuchs Tina
Published: September 2023

This report demonstrates a novel class of innate immune cells designated “variable immunoreceptor–expressing myeloids” (VIREMs). Using single-cell transcriptomics and genome-wide epigenetic profiling, we establish that VIREMs are myeloid cells unrelated to lymphocytes. We visualize the phenotype of B-VIREMs that are capable of genetically recombining and expressing antibody genes, the exclusive hallmark function of B lymphocytes. These cells, designated B-VIREMs, display monoclonal antibody cell surface signatures and regularly circulate in the blood of healthy individuals. Single-cell data reveal clonal expansion of circulating B-VIREMs as a dynamic response to disease stimuli. Live-cell imaging models suggest that B-VIREMs load their own Fc receptors with endogenous antibodies during vesicle transport to the cell surface. A first cloned B-VIREM–derived antibody (Vab1) specifically binds stomatin, a ubiquitous scaffold protein that is strictly expressed intracellularly, allowing Vab1-bearing macrophages to phagocytose cell debris without requiring prior opsonization. Our results suggest important antigen-specific tissue maintenance functionalities in these innate immune cells.
Butyrate enhances
Clostridioides difficile
sporulation
in vitro
Authors: Baldassare Michelle A. , Bhattacharjee Disha , Coles Julian D. , Nelson Sydney , McCollum C. Alexis , Seekatz Anna M.
Published: September 2023

Short-chain fatty acids (SCFAs) are products of bacterial fermentation that help maintain important gut functions such as maintenance of the intestinal barrier, cell signaling, and immune homeostasis. The main SCFAs acetate, propionate, and butyrate have demonstrated beneficial effects for the host, including its importance in alleviating infections caused by pathogens such as
Clostridioides difficile
. Despite the potential role of SCFAs in mitigating
C. difficile
infection, their direct effect on
C. difficile
remains unclear. Through a set of
in vitro
experiments, we investigated how SCFAs influence
C. difficile
growth, sporulation, and toxin production. Similar to previous studies, we observed that butyrate decreased growth of
C. difficile
strain 630 in a dose-dependent manner. The presence of butyrate also increased
C. difficile
sporulation, with minimal increases in toxin production. RNA-Seq analysis validated our experimental results, demonstrating increased expression of sporulation-related genes in conjunction with changes in metabolic and regulatory genes, such as a putative carbon starvation protein, CstA. Collectively, these data suggest that butyrate may induce alternative
C. difficile
survival pathways, modifying its growth ability and virulence to persist in the gut environment.

IMPORTANCE

Several studies suggest that butyrate may modulate gut infections, such as reducing inflammation caused by the healthcare-associated
Clostridioides difficile
. While studies in both animal models and human studies correlate high levels of butyrate with reduced
C. difficile
burden, the direct impact of butyrate on
C. difficile
remains unclear. Our study demonstrates that butyrate directly influences
C. difficile
by increasing its sporulation and modifying its metabolism, potentially using butyrate as a biomarker to shift survival strategies in a changing gut environment. These data point to additional therapeutic approaches to combat
C. difficile
in a butyrate-directed manner.
Boosting of vaginal HSV-2-specific B and T cell responses by intravaginal therapeutic immunization results in diminished recurrent HSV-2 disease
Authors: Bourne Nigel , Keith Celeste A. , Miller Aaron L. , Pyles Richard B. , Cohen Gary , Milligan Gregg N.
Published: September 2023

Boosting herpes simplex virus (HSV)-specific immunity in the genital tissues of HSV-positive individuals to increase control of HSV-2 recurrent disease and virus shedding is an important goal of therapeutic immunization and would impact HSV-2 transmission. Experimental therapeutic HSV-2 vaccines delivered by a parenteral route have resulted in decreased recurrent disease in experimental animals. We used a guinea pig model of HSV-2 infection to test if HSV-specific antibody and cell-mediated responses in the vaginal mucosa would be more effectively increased by intravaginal (Ivag) therapeutic immunization compared to parenteral immunization. Therapeutic immunization with HSV glycoproteins and CpG adjuvant increased glycoprotein-specific IgG titers in vaginal secretions and serum to comparable levels in Ivag- and intramuscular (IM)-immunized animals. However, the mean numbers of HSV glycoprotein-specific antibody secreting cells (ASCs) and IFN-γ SCs were greater in Ivag-immunized animals demonstrating superior boosting of immunity in the vaginal mucosa compared to parenteral immunization. Therapeutic Ivag immunization also resulted in a significant decrease in the cumulative mean lesion days compared to IM immunization. There was no difference in the incidence or magnitude of HSV-2 shedding in either therapeutic immunization group compared to control-treated animals. Collectively, these data demonstrated that Ivag therapeutic immunization was superior compared to parenteral immunization to boost HSV-2 antigen-specific ASC and IFN-γ SC responses in the vagina and control recurrent HSV-2 disease. These results suggest that novel antigen delivery methods providing controlled release of optimized antigen/adjuvant combinations in the vaginal mucosa would be an effective approach for therapeutic HSV vaccines.

IMPORTANCE

HSV-2 replicates in skin cells before it infects sensory nerve cells where it establishes a lifelong but mostly silent infection. HSV-2 occasionally reactivates, producing new virus which is released back at the skin surface and may be transmitted to new individuals. Some HSV-specific immune cells reside at the skin site of the HSV-2 infection that can quickly activate and clear new virus. Immunizing people already infected with HSV-2 to boost their skin-resident immune cells and rapidly control the new HSV-2 infection is logical, but we do not know the best way to administer the vaccine to achieve this goal. In this study, a therapeutic vaccine given intravaginally resulted in significantly better protection against HSV-2 disease than immunization with the same vaccine by a conventional route. Immunization by the intravaginal route resulted in greater stimulation of vaginal-resident, virus-specific cells that produced antibody and produced immune molecules to rapidly clear virus.
Size Isomers at Threshold States
Authors: Demyanova A. S. , Danilov A. N. , Starastsin V. I. , Goncharov S. A. , Gurov Yu. B.
Published: September 2023

An overview is provided for a new class of states, which we have named as the size isomers. Such states are weakly bound and have an exotic structure (cluster states, halo/‘‘skin’’). The development of methods for measuring the radii of nuclei in their short-lived excited states led to the discovery of new classes of states. The size of a nucleus defined by the radius of its nucleon (proton and neutron) density distribution and the proton charge distribution is one of the most fundamental and important nuclear characteristics. Nuclear radius determines the basic properties of nuclei and is a consequence of the fundamental features of the strong interaction. Up to now two groups of the size isomers were identified: the excited states with halos ($${}^{9}$$Be, $${}^{11}$$Be, $${}^{13}$$C, $${}^{13}$$N) and some specific alpha-cluster states ($${}^{11}$$B, $${}^{12}$$C, $${}^{13}$$C). All the observed states are dilute, however, some indication to possible existence of more compact than the ground states was obtained as well (in $${}^{13}$$C). The phenomenon of size isomerism occurred to be not a rare one especially if we take into account rotational bands based on some of such states. The structure of size isomers is related with some new features, e.g., rotating halos, halos in continuum, different types of quasimolecular configurations. Some rudimentary signs of alpha-particle condensation (a ‘‘ghost’’ of condensate) were observed in the Hoyle state of $${}^{12}$$C, however, we cannot speak about confirmation of this ambitious theory. Analogs to the Hoyle state, expected in $${}^{11}$$B, $${}^{13}$$C, $${}^{16}$$O, $${}^{20}$$Ne near the $$\alpha$$-emission thresholds, are considered.
Social health: rethinking the concept through social practice theory and feminist care ethics
Authors: Paul Joshua , Merz Sibille , Bergholz Andreas , König Franziska , Weigt Julia , Eich-Krohm Astrid , Apfelbacher Christian , Holmberg Christine
Published: September 2023

The social sciences have long shown that health is not born of pure biology, empirically (re)centred the social and material causes of disease, and affirmed the subjective experiences of disease. Disputed both in popular and academic discourses, social health has variously attempted to stress the social aspects of health. Existing conceptions remain analytically limited as they are predominantly used as descriptors for populational health. This article theorises social health as an analytical lens for making sense of the relations, affects and events where health unfolds and comes into expression. Drawing on social practice theory, feminist care ethics and posthumanism this conceptual paper re-imagines how social health might be conceived as lived social practices anchored in care. Care within our framework acknowledges the unavoidable interdependency foundational to the existence of beings and stresses the ‘know how’ and embodied practices of care in the mundane in order to emphasise that care itself is absolutely integral to the maintenance of social health. The article argues that health needs to be understood as a verb intrinsically (re)made in and through social contexts and structures and comprised of meaningful, human-human and human-non-human interactions. Ultimately, in theorising social health through mundane care practices, we hope to open up research to making sense of how the doing of health unfolds inside often banal, patterned forms of social activity. Such taken-for-granted social practices exemplify the often overlooked lived realities that comprise our health. To understand health in its own right, we argue, these everyday practices need to be interrogated.
Comparison of injectate spread and nerve coverage between single-injection intertransverse process block and paravertebral block at the T2 level: a cadaveric study
Authors: Kim Ji Yeong , Lee U-Young , Kim Do-Hyeong , Han Dong Woo , Kim Sang Hyun , Cho Yun Jeong , Jeong Hyeyoon , Kim Yun Ji , Yang A Rim , Park Hue Jung
Published: September 2023

Background

We compared the spread of an injectate into the thoracic sympathetic chain resulting from a single-injection intertransverse process (ITP) block with that of a single-injection PV block at the T2 level.

Methods

Sixteen soft-embalmed cadavers were used. The right hemithorax was randomly allocated to receive either an ultrasound-guided single-injection ITP block or ultrasound-guided single-injection PV block at the T2 vertebral level, with the opposite block administered to the other side. Approximately 10 mL of latex dye solution was injected into each hemithorax using a random allocation technique. The presence of dye at the nerve root in the sympathetic chain and intercostal nerves at the injection and additional levels was examined by dissection.

Results

The injectate spread into the T2 sympathetic ganglion on both ITP (11/16, 68.8%) and PV (16/16, 100%) blocks. The ITP block demonstrated greater uniformity of dye staining in both the dorsal rami and dorsal root ganglion, which contrasts with the less consistent staining outcomes of the PV block in these regions.

Conclusions

At the T2 level, we observed a lower efficacy of the ITP block for analgesic coverage of the sympathetic nerve. This suggested a potential preference by clinicians for the application of the T2 PV block over the ITP block, specifically for the management of sympathetically maintained pain in the upper extremities. In addition, our findings may hint at the potential advantages of the ITP block in specific clinical contexts where targeted nerve involvement, such as the medial branch block or dorsal root ganglion block, is sought.
Amiodarone prevents wave front-tail interactions in patients with heart failure: an in silico study
Authors: Gray Richard A. , Franz Michael R.
Published: September 2023

This work presents a new model of the action potential of the human, which reproduces the complex dynamics during premature stimulation in heart failure patients with and without amiodarone. A specific mechanism of the ability of amiodarone to prevent reentrant arrhythmias is presented.
Supplementary Data from A Nonconserved Histidine Residue on KRAS Drives Paralog Selectivity of the KRASG12D Inhibitor MRTX1133
Authors: Keats Miles A. , Han John J.W. , Lee Yeon-Hwa , Lee Chih-Shia , Luo Ji
Published: September 2023

Supplementary discussion, supplementary tables and figures.
Supplementary Tables S3-5 from Activation of the cGAS/STING Axis in Genome-Damaged Hematopoietic Cells Does Not Impact Blood Cell Formation or Leukemogenesis
Authors: Dressel Nicole , Natusch Loreen , Munz Clara M. , Costas Ramon Santiago , Morcos Mina N.F. , Loff Anja , Hiller Björn , Haase Christa , Schulze Livia , Müller Patrick , Lesche Mathias , Dahl Andreas , Luksch Hella , Rösen-Wolff Angela , Roers Axel , Behrendt Rayk , Gerbaulet Alexander
Published: September 2023

Transcriptome analysis
Supplementary Data from Activation of the cGAS/STING Axis in Genome-Damaged Hematopoietic Cells Does Not Impact Blood Cell Formation or Leukemogenesis
Authors: Dressel Nicole , Natusch Loreen , Munz Clara M. , Costas Ramon Santiago , Morcos Mina N.F. , Loff Anja , Hiller Björn , Haase Christa , Schulze Livia , Müller Patrick , Lesche Mathias , Dahl Andreas , Luksch Hella , Rösen-Wolff Angela , Roers Axel , Behrendt Rayk , Gerbaulet Alexander
Published: September 2023

Supplementary Figures S1-8 and corresponding figure legends
Supplementary Table S1 from Supraphysiological Androgens Promote the Tumor Suppressive Activity of the Androgen Receptor through cMYC Repression and Recruitment of the DREAM Complex
Authors: Nyquist Michael D. , Coleman Ilsa M. , Lucas Jared M. , Li Dapei , Hanratty Brian , Meade Hannah , Mostaghel Elahe A. , Plymate Stephen R. , Corey Eva , Haffner Michael C. , Nelson Peter S.
Published: September 2023

Table of genes comprising signatures.
Supplementary Video 4 from Targeting CaMKK2 Inhibits Actin Cytoskeletal Assembly to Suppress Cancer Metastasis
Authors: Mukherjee Debarati , Previs Rebecca A. , Haines Corinne , Al Abo Muthana , Juras Patrick K. , Strickland Kyle C. , Chakraborty Binita , Artham Sandeep , Whitaker Regina S. , Hebert Katherine , Fontenot Jake , Patierno Steven R. , Freedman Jennifer A. , Lau Frank H. , Burow Matthew E. , Chang Ching-Yi , McDonnell Donald P.
Published: September 2023

Supplementary Video 4
Supplementary Video 1 from Targeting CaMKK2 Inhibits Actin Cytoskeletal Assembly to Suppress Cancer Metastasis
Authors: Mukherjee Debarati , Previs Rebecca A. , Haines Corinne , Al Abo Muthana , Juras Patrick K. , Strickland Kyle C. , Chakraborty Binita , Artham Sandeep , Whitaker Regina S. , Hebert Katherine , Fontenot Jake , Patierno Steven R. , Freedman Jennifer A. , Lau Frank H. , Burow Matthew E. , Chang Ching-Yi , McDonnell Donald P.
Published: September 2023

Supplementary Video 1
Supplementary Figures 1-4 and Tables 1-2 from Targeting CaMKK2 Inhibits Actin Cytoskeletal Assembly to Suppress Cancer Metastasis
Authors: Mukherjee Debarati , Previs Rebecca A. , Haines Corinne , Al Abo Muthana , Juras Patrick K. , Strickland Kyle C. , Chakraborty Binita , Artham Sandeep , Whitaker Regina S. , Hebert Katherine , Fontenot Jake , Patierno Steven R. , Freedman Jennifer A. , Lau Frank H. , Burow Matthew E. , Chang Ching-Yi , McDonnell Donald P.
Published: September 2023

Contains Supplementary Figures 1-4, Supplementary Tables 1-2 and legends for Supplementary Videos 1-4
Table S4 from Mesenchymal-like Tumor Cells and Myofibroblastic Cancer-Associated Fibroblasts Are Associated with Progression and Immunotherapy Response of Clear Cell Renal Cell Carcinoma
Authors: Davidson Guillaume , Helleux Alexandra , Vano Yann A. , Lindner Véronique , Fattori Antonin , Cerciat Marie , Elaidi Reza T. , Verkarre Virginie , Sun Cheng-Ming , Chevreau Christine , Bennamoun Mostefa , Lang Hervé , Tricard Thibault , Fridman Wolf H. , Sautes-Fridman Catherine , Su Xiaoping , Plassard Damien , Keime Celine , Thibault-Carpentier Christelle , Barthelemy Philippe , Oudard Stéphane M. , Davidson Irwin , Malouf Gabriel G.
Published: September 2023

Deconvolution of cell types in BIONIKK cohort.
Table S2 from Mesenchymal-like Tumor Cells and Myofibroblastic Cancer-Associated Fibroblasts Are Associated with Progression and Immunotherapy Response of Clear Cell Renal Cell Carcinoma
Authors: Davidson Guillaume , Helleux Alexandra , Vano Yann A. , Lindner Véronique , Fattori Antonin , Cerciat Marie , Elaidi Reza T. , Verkarre Virginie , Sun Cheng-Ming , Chevreau Christine , Bennamoun Mostefa , Lang Hervé , Tricard Thibault , Fridman Wolf H. , Sautes-Fridman Catherine , Su Xiaoping , Plassard Damien , Keime Celine , Thibault-Carpentier Christelle , Barthelemy Philippe , Oudard Stéphane M. , Davidson Irwin , Malouf Gabriel G.
Published: September 2023

Single-cell RNA sequencing markers for each global cluster and each re-clustering
Table S1 from Mesenchymal-like Tumor Cells and Myofibroblastic Cancer-Associated Fibroblasts Are Associated with Progression and Immunotherapy Response of Clear Cell Renal Cell Carcinoma
Authors: Davidson Guillaume , Helleux Alexandra , Vano Yann A. , Lindner Véronique , Fattori Antonin , Cerciat Marie , Elaidi Reza T. , Verkarre Virginie , Sun Cheng-Ming , Chevreau Christine , Bennamoun Mostefa , Lang Hervé , Tricard Thibault , Fridman Wolf H. , Sautes-Fridman Catherine , Su Xiaoping , Plassard Damien , Keime Celine , Thibault-Carpentier Christelle , Barthelemy Philippe , Oudard Stéphane M. , Davidson Irwin , Malouf Gabriel G.
Published: September 2023

General and specific markers for the identification of each sub-cluster in scRNAseq analysis and associated references.
Supplementary Figure Annotations from Mesenchymal-like Tumor Cells and Myofibroblastic Cancer-Associated Fibroblasts Are Associated with Progression and Immunotherapy Response of Clear Cell Renal Cell Carcinoma
Authors: Davidson Guillaume , Helleux Alexandra , Vano Yann A. , Lindner Véronique , Fattori Antonin , Cerciat Marie , Elaidi Reza T. , Verkarre Virginie , Sun Cheng-Ming , Chevreau Christine , Bennamoun Mostefa , Lang Hervé , Tricard Thibault , Fridman Wolf H. , Sautes-Fridman Catherine , Su Xiaoping , Plassard Damien , Keime Celine , Thibault-Carpentier Christelle , Barthelemy Philippe , Oudard Stéphane M. , Davidson Irwin , Malouf Gabriel G.
Published: September 2023

Annotation of supplementary figures
Supplementary Tables from Preexisting Immunity Drives the Response to Neoadjuvant Chemotherapy in Esophageal Adenocarcinoma
Authors: Arbore Giuseppina , Albarello Luca , Bucci Gabriele , Punta Marco , Cossu Andrea , Fanti Lorella , Maurizio Aurora , Di Mauro Francesco , Bilello Vito , Arrigoni Gianluigi , Bonfiglio Silvia , Biancolini Donatella , Puccetti Francesco , Elmore Ugo , Vago Luca , Cascinu Stefano , Tonon Giovanni , Rosati Riccardo , Casorati Giulia , Dellabona Paolo
Published: September 2023

Supplementary Table 1. Clinical characteristics of the EAC patients from the prospective cohort (protocol ESO-CA001)
Supplementary Table 2. Clinical characteristics of the EAC patients from the additional retrospective cohort selected for immunohistochemistry and digital spatial profiling
Supplementary Table 3. Monoclonal antibodies used for immunohistochemistry
Supplementary Table 4. Monoclonal antibodies used for flow cytometry staining
Supplementary Table 5. Baseline mutation in genes within the Reactome MHC class I antigen processing in EAC tumors from the prospective cohort
Supplementary Table 6. Baseline mutation in genes within the EAC Driver Genes in EAC tumors from the prospective cohort
Supplementary Figures from Preexisting Immunity Drives the Response to Neoadjuvant Chemotherapy in Esophageal Adenocarcinoma
Authors: Arbore Giuseppina , Albarello Luca , Bucci Gabriele , Punta Marco , Cossu Andrea , Fanti Lorella , Maurizio Aurora , Di Mauro Francesco , Bilello Vito , Arrigoni Gianluigi , Bonfiglio Silvia , Biancolini Donatella , Puccetti Francesco , Elmore Ugo , Vago Luca , Cascinu Stefano , Tonon Giovanni , Rosati Riccardo , Casorati Giulia , Dellabona Paolo
Published: September 2023

Supplementary Figures S1 to S14
Supplementary Figure 1. Multidimensional analysis of baseline EAC tumor and blood samples
Supplementary Figure 2. Baseline higher immune gene signatures and reduced resistance to stress are associated with response to neoadjuvant chemotherapy.
Supplementary Figure 3. Immunohistochemistry for Foxp3, CD163, CD45RO performed on treatment-naïve EACs
Supplementary Figure 4. Immunohistochemistry on treatment-naïve EACs for CD3, CD4, CD8 and CD68
Supplementary Figure 5. Digital spatial profiling of the proteome in treatment-naïve EACs
Supplementary Figure 6. Panels for flow cytometry analysis of T lymphocytes
Supplementary Figure 7. High-dimensional flow cytometry analysis of CD4+ tumorinfiltrating lymphocytes
Supplementary Figure 8. High-dimensional flow cytometry analysis of CD8+ tumorinfiltrating lymphocytes
Supplementary Figure 9. Panels showing flow cytometry analysis of CD3- immune cell subsets
Supplementary Figure 10. High-dimensional flow cytometry analysis of tumor-infiltrating CD45+CD3- cells
Supplementary Figure 11. High-dimensional flow cytometry analysis of circulating CD4+ lymphocytes
Supplementary Figure 12. High-dimensional flow cytometry analysis of circulating CD8+ lymphocytes
Supplementary Figure 13. High-dimensional flow cytometry analysis of circulating CD45+CD3- cells
Supplementary Figure 14. Concentrations of plasma cytokines in EAC patients before neoadjuvant chemotherapy
Table S5 from Breast Cancer Mutations HER2V777L and PIK3CAH1047R Activate the p21–CDK4/6–Cyclin D1 Axis to Drive Tumorigenesis and Drug Resistance
Authors: Cheng Xiaoqing , Sun Yirui , Highkin Maureen , Vemalapally Nagalaxmi , Jin Xiaohua , Zhou Brandon , Prior Julie L. , Tipton Ashley R. , Li Shunqiang , Iliuk Anton , Achilefu Samuel , Hagemann Ian S. , Edwards John R. , Bose Ron
Published: September 2023

Phosphoproteins in Heatmap
Table S3 from Breast Cancer Mutations HER2V777L and PIK3CAH1047R Activate the p21–CDK4/6–Cyclin D1 Axis to Drive Tumorigenesis and Drug Resistance
Authors: Cheng Xiaoqing , Sun Yirui , Highkin Maureen , Vemalapally Nagalaxmi , Jin Xiaohua , Zhou Brandon , Prior Julie L. , Tipton Ashley R. , Li Shunqiang , Iliuk Anton , Achilefu Samuel , Hagemann Ian S. , Edwards John R. , Bose Ron
Published: September 2023

Phosphoproteins in Volcano Plot
Table S3 from CD36 Drives Metastasis and Relapse in Acute Myeloid Leukemia
Authors: Farge Thomas , Nakhle Jean , Lagarde Damien , Cognet Guillaume , Polley Nathaniel , Castellano Rémy , Nicolau Marie-Laure , Bosc Claudie , Sabatier Marie , Sahal Ambrine , Saland Estelle , Jeanson Yannick , Guiraud Nathan , Boet Emeline , Bergoglio Camille , Gotanègre Mathilde , Mouchel Pierre-Luc , Stuani Lucille , Larrue Clément , Sallese Marie , De Mas Véronique , Moro Cedric , Dray Cédric , Collette Yves , Raymond-Letron Isabelle , Ader Isabelle , Récher Christian , Sarry Jean-Emmanuel , Cabon Florence , Vergez François , Carrière Audrey
Published: September 2023

Single cell analysis
Table S2 from Breast Cancer Mutations HER2V777L and PIK3CAH1047R Activate the p21–CDK4/6–Cyclin D1 Axis to Drive Tumorigenesis and Drug Resistance
Authors: Cheng Xiaoqing , Sun Yirui , Highkin Maureen , Vemalapally Nagalaxmi , Jin Xiaohua , Zhou Brandon , Prior Julie L. , Tipton Ashley R. , Li Shunqiang , Iliuk Anton , Achilefu Samuel , Hagemann Ian S. , Edwards John R. , Bose Ron
Published: September 2023

Phosphopeptides