bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis Butir Soal Ujian Akhir Semester Ganjil Bahasa Indonesia Kelas X Tahun Ajaran 2022/2023 di SMAN 2 Jember
Authors: Humaria Intan Usrifatul , Muti'ah Arju , Syukron Ahmad
Published: July 2024

Abstrak 
Penelitian ini bertujuan untuk mengetahui validitas isi, tingkat kesukaran, dan analisis daya beda pada soal ujian akhir semester ganjil bahasa Indonesia kelas X di SMAN 2 Jember. Jenis penelitian ini yaitu penelitian evaluatif. Menurut Kantun (2017) penelitian evaluatif merupakan proses penelitian yang memiliki sifat mengevaluasi kegiatan atau program yang memiliki tujuan untuk menilai keberhasilan program yang sudah dibuat sebelumnya. Data dalam penelitian ini berupa angka- angka dan pemaparan kalimat yang merupakan nilai dari hasil pehritungan rumusan masalah. Sumber data dalam penelitian ini berupa: kisi- kisi soal, kartu soal dan lembar soal ujian akhir semester ganjil bahasa Indonesia kelas X dan nilai ujian akhir semester ganjil bahasa Indonesia kelas X. Teknik pengumpulan data yaitu observasi dan wawancara. Hasil penelitian menunjukkan bahwa validitas soal yang dibuat berada pada kategori baik, sedangkan untuk tingkat kesukaran soal berada pada kategori rendah dan perlu diperbaiki, dan daya beda masih berada pada kategori perlu  diperbaiki/ direvisi, karena belum dapat membedakan kemampuan siswa kategori atas dan kategori bawah.
Kata Kunci: Butir Soal, Validitas Isi, Daya Beda, dan Tingkat Kesukaran 
Abstarct 
This study aims to determine content validity, level of difficulty, and analysis of differential power in the final exam questions for odd semester Indonesian in class X at SMAN 2 Jember. This type of research is evaluative research. The data in this study are in the form of numbers and sentence exposure which is the value of the calculation results of the problem formulation. Sources of data in this study were: a grid of questions, question cards and question sheets at the end of the odd semester of Indonesian for class X and grades for the final exam for odd semester for class X. Data collection techniques were observation and interviews. The results showed that the validity of the questions made was in the good category, while the level of difficulty and discriminating power were still in the category that needed to be repaired/revised, because they could not distinguish between the abilities of the upper and lower categories of students.
Keywords :Items, Content Validity, Distinction, and Difficulty Level
ANALISIS KELAYAKAN KEBAHASAAN DAN KEGRAFIKAN MATERI IKLAN DALAM BUKU TEKS BAHASA INDONESIA KURIKULUM MERDEKA KELAS VIII SMP
Authors: Damayanti Nia , Lestari Sri , Hardini Nadhiva Shafa , Muti'ah Arju , Muji Muji
Published: July 2024

Kehadiran buku teks tidak dapat dilepaskan dari kegiatan belajar dan mengajar. Buku teks digunakan sebagai panduan penyampaian materi bahan ajar. Maka dari itu, penyusunan buku teks harus disesuaikan dengan standar dan kelayakan buku. Penilaian kelayakan buku teks salah satunya dilihat dari aspek kebahasaan dan kegrafikan pada buku teks. Tujuan penelitian ini adalah untuk mendeskripsikan kelayakan kebahasaan dan kegrafikan buku teks Bahasa Indonesia kelas VIII Kurikulum Merdeka. Penelitian ini digunakan untuk membantu guru dalam memilih buku teks yang sesuai standar kelayakan dan memberikan sumbangsih dalam pengembangan buku teks bahasa Indonesia. Metode yang digunakan penelitian ini adalah metode kualitatif, sumber data yang digunakan adalah Buku take Bahasa Indonesia Kurikulum Merdeka kelas VIII SMP dengan teknik pengumpulan menggunakan teknik dokumentasi. Analisis data penelitian ini terdiri dari reduksi data, penyajian data, dan penarikan kesimpulan. Hasil dari analisis kelayakan buku teks pada materi iklan telah memenuhi komponen penting yaitu, komunikatif, dialogis dan interaktif, lugas, keruntutan alur pikir, koherensi, kesesuaian dengan kaidah bahasa indonesia, serta kesesuaian ikon dan simbol. Sedangkan analisis kegtalikan buku teks telah sesuai dengan ukuran format buku, desain kulit dan isi, bentuk paragraf, serta ilustrasi. Secara keseluruhan buku teks Bahasa Indonesia Kurikulum Merdeka kelas VIII SMP pada materi iklan telah memenuhi semua penilaian kebahasaan maupun kegrafikan.
Pandemic Considerations in Literacy Research & Teaching
Authors: Burkholder Casey , Brubacher Katie
Published: July 2024

Editors note: The remaining articles are from the 2022 Pre-Conference of LLRC and have been placed within this special issue of L&L.
It takes a village
Authors: Kalindi Sylvia Chanda , Brigham Susan , Frempong George
Published: July 2024

Several stakeholders, such as community groups, government officials, parents, guardians, and educators have shown concern about the achievement gap between Nova Scotia learners of African descent and their predominantly White peers. Using the African proverb ‘It takes a village’ as a framework, this study involved parents, extended family members and caregivers of children aged 4 to 6 years to explore the scaffolding strategies employed as well as the experiences of participants when supporting children’s emergent writing skills during COVID-19 pandemic. Survey and focus group sessions demonstrated how participants frequently scaffolded emergent writing using highly effective strategies including Africentric approaches. The importance of ‘It takes a village’ proverb in enhancing literacy developmenthas also been established.
ANALYSIS OF ROUTING PROTOCOLS CHARACTERISTICS IN AD-HOC NETWORK
Authors: Hryschuk Iryna , Astrakhantsev Andrii , Pedan Stanislav , Globa Larysa
Published: July 2024

Background. Wireless ad-hoc networks are becoming increasingly prevalence in remote areas, in extreme environments, even in military operations, and in scenarios where setting up infrastructure networks is not possible. Research of ad-hoc routing protocols problems allows improving the efficiency of their operation in conditions of high variability in packet loss or instability of network operation when the speed of users changes.
Objective. The purpose of the paper is analysis of packet loss dependency from a network operation time, study of a user speed influence on a network efficiency, and research of network operation efficiency with different routing protocols.
Methods. The method of routing protocols efficiency evaluation is the simulation of their operation in an ad-hoc network on a test data set and research of a network indicators dependency in time under different loads and changing mobility of users.
Results. The conducted research demonstrated that user’s mobility at different speeds significantly affects the network operation as a whole. The instability of users' positions leads to a significant increase in route search time and packet transmission time. Among researched GPSR, DSDV, and AODV protocols, the latter proved to be the best because it has the lowest percentage of data loss and the lowest average time of message send and receive operations.
Conclusions. The work is dedicated to the actual problem of developing and setting parameters of ad-hoc network. Received research results indicate the need to choose the optimal routing protocol depending on specific application conditions, such as user movement speed and network stability. The proposed solutions can be the first stage of complex processing of packets in the mobile network and justify the choice of AODV protocol as a basis for further improvement.
As estratégias de hedging na política de segurança sul-coreana: uma análise realista neoclássica sobre as percepções sul-coreanas acerca da China entre 2013-2022
Authors: Nummer Martha Silveira , Oliveira Alana Camoça Gonçalves de
Published: July 2024

O artigo analisa a estratégia de política externa da Coreia do Sul para a China durante os governos de Park Geun-hye(2013-2016) e Moon Jae-in(2017-2022) por meio dos Livros Brancos de Defesa da Coreia do Sul e documentos oficiais durante o período supramencionado. Objetiva-se compreender se a Coreia do Sul utiliza a política de hedge nas suas relações com a China para garantir estabilidade estratégica e prosperidade econômica.
SeqMate: A Novel Large Language Model Pipeline for Automating RNA Sequencing
Authors: Mondal Devam , Inamdar Atharva
Published: July 2024

RNA sequencing techniques, like bulk RNA-seq and Single Cell (sc) RNA-seq, are critical tools for the biologist looking to analyze the genetic activity/transcriptome of a tissue or cell during an experimental procedure. Platforms like Illumina's next-generation sequencing (NGS) are used to produce the raw data for this experimental procedure. This raw FASTQ data must then be prepared via a complex series of data manipulations by bioinformaticians. This process currently takes place on an unwieldy textual user interface like a terminal/command line that requires the user to install and import multiple program packages, preventing the untrained biologist from initiating data analysis. Open-source platforms like Galaxy have produced a more user-friendly pipeline, yet the visual interface remains cluttered and highly technical, remaining uninviting for the natural scientist. To address this, SeqMate is a user-friendly tool that allows for one-click analytics by utilizing the power of a large language model (LLM) to automate both data preparation and analysis (differential expression, trajectory analysis, etc). Furthermore, by utilizing the power of generative AI, SeqMate is also capable of analyzing such findings and producing written reports of upregulated/downregulated/user-prompted genes with sources cited from known repositories like PubMed, PDB, and Uniprot.
Developing a Symbiotic Fermented Milk Product with Microwave-Treated Hawthorn Extract
Authors: Utebaeva Aidana , Gabrilyants Eleonora , Abish Zhansaya
Published: July 2024

The rising interest in functional foods has increased the use of probiotics and prebiotics in fermented dairy products to enhance gut health. This study focuses on developing a symbiotic fermented milk product using Lactobacillus acidophilus and Bifidobacterium bifidum activated with hawthorn extract as a prebiotic. Three versions of the product were tested: a control, and two variants with B. bifidum activated with 10-5 g/cm³ and 10-10 g/cm³ hawthorn extract, respectively. Key characteristics such as microbiological safety, sensory properties, amino acid profile, vitamin and mineral content, antioxidant capacity, and nutritional values were evaluated. Results showed that products enriched with hawthorn extract had favorable sensory properties and sustained high levels of lactic acid bacteria while being free of pathogens. Product 1 demonstrated significant increases in L. acidophilus (24.1%) and B. bifidum (14.7%) after 7 days compared to the control. Both enriched products exhibited slower titratable acidity increases and higher viscosities over 14 days, indicating better preservation and texture stability. Product 1 was notably enriched with essential amino acids, vitamins, and minerals, alongside enhanced antioxidant properties due to increased flavonoid content. The technology developed ensures probiotic viability at 109 - 1010 CFU/cm³ after 14 days, making it viable for dairy production.
A transzlingváló pedagógiai orientáció hatása az iskolai sikerességre
Authors: Heltai János Imre , Jani-Demetriou Bernadett
Published: July 2024

Bár a transzlingválás a kortárs szociolingvisztika egyik meghatározó koncepciója, empirikus vizsgálatára mindeddig kevés kísérlet történt. Ez a dolgozat a Transzlingvális osztálytermi kommunikáció és hatékony tanulásszervezés Tiszavasváriban elnevezésű nyelvpedagógiai programot, valamint a programhoz tartozó mérések eredményeit mutatja be. A program célja kétnyelvű roma gyerekek iskolai sikerességének a növelése. Mérési eredményeink szerint a transzlingválásnak nevezett pedagógiai orientáció alkalmazása mérhető pozitív hatással van a gyerekek általános és egyes nyelvekhez köthető nyelvi kompetenciáira is.  Azok az elsősök, akiknek oktatásában a transzlingválás nagyobb teret kapott, év végén nemcsak jobb eredményeket értek el mind romani, mind magyar nyelvű szövegalkotási és szövegértési feladatokban, hanem szívesebben is szólaltak meg az iskola tannyelvén. A mérések további fontos eredménye, hogy az iskolakezdéskor felvett tesztek mindegyikében jobban teljesítettek azok a gyerekek, akiknek az otthoni nyelvi szocializációja a család kétnyelvű megszólalási módjait követi, mint azok, akiknek nyelvi szocializációját a szülők – a társadalmi elvárásoknak való megfelelés szándékával – a többségi (magyar) nyelvhez igyekeztek kötni.
Esquema del sistema constitucional de Derechos, Libertades y Principios Rectores
Authors: Gómez Puerto Ángel
Published: July 2024

La triple lista de derechos y libertades del sistema establecido en la constitución de 1978
Contemporary Self-identification as a “Tutejszy” in Southeastern Lithuania
Authors: Vyšniauskas Vidmantas , Pilarczyk-Palaitis Anna
Published: July 2024

The article raises the issue of identification as a “tutejszy” among people who live in southeastern Lithuania today. The question of how and why “tutejsi” (plural for “tutejszy”) draw identity boundaries between themselves and other people is very important due to the overall social and political changes in this region over the last century. To answer this question, qualitative field research was conducted using observation, participant observation, and in-depth interviews with the region’s inhabitants. The data analysis revealed two categories that play a role in the drawing of identity boundaries: the first relates to ethnicity and the second one relates to the value ascribed to being a “tutejszy”. Based on these two axes, the authors developed four models of “tutejszy” identification in southeastern Lithuania.
WHY SHOULD HISTORIANS NOT DISREGARD GENEALOGY? FROM FAMILY HISTORY AND FAMILY MEMORY TO INVESTIGATIVE GENETIC GENEALOGY
Authors: Švaříčková Slabáková Radmila
Published: July 2024

The aim of this study is twofold. On the one hand, the article provides an overview of current research in the field of family memory, amateur family history and (genetic) genealogy, emphasizing a few areas that are of interest for professional historians. These include the close links between the stories transmitted in families and family identity, its functioning and family resilience; the potential of family history to reveal alternative versions of the national past; and finally, genetic (DNA) genealogy, which has won the favour of tens of millions of adherents worldwide and which is able to redefine historically traditional social structures such as family or kinship. Investigative genetic genealogy is a fresh and burgeoning field that gives amateur family history new dimensions by including it into the investigative leads of law enforcement authorities. In the second line, the article provides examples of research done in the Czech Republic.
The Phenomenon of COVID-19 Conspiracy Theory Narratives through Virtual Ethnography on Instagram
Authors: Qorib Fathul , Ramadan Rangga Tirta
Published: July 2024

This study aims to investigate the conspiracy phenomenon of the COVID-19 pandemic as disseminated by the Instagram account @unexplnd. This account boasts 842 thousand followers and stands as one of the largest social media propagating the concept of COVID-19 conspiracy theories in Indonesia. Primary data is derived from posts on this account, and secondary data is obtained through interviews with four followers who actively engage in commenting and harbor beliefs in the existence of conspiracies. The research corpus is subsequently scrutinized employing the conceptual framework delineated by Douglas, Sutton, and Cichocka (2017), which delineates the emergence of conspiracies through epistemic, existential, and social motives. The findings of this investigation reveal that incidents perceived as random chance occurrences, motivated by the desire to preserve comfort and freedom, along with suspicion or resentment towards particular entities, serve as grounds for endorsing COVID-19 pandemic conspiracy theories. Furthermore, exposure to media presenting conspiracy theories, such as the Instagram account @unexplnd, contributes to an augmented belief in such theories among proponents. Conspiracy theorists desire to raise awareness among the general populace regarding conspiracy theories by disseminating information containing conspiracy narratives across various media channels.
WITHDRAWN: Trade engagement, economic growth, and institutional quality on environmental degradation for ASEAN-5 under the Green Belt-Road Initiative
Authors: Huang Di , Loganathan Nanthakumar , Subranmaniam Yogeeswari , Kogid Mori
Published: July 2024

The full text of this preprint has been withdrawn by the authors as it was submitted and made public without the full consent of all the authors. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Imaging mofette structures in the Ohře Rift System, Czech Republic, using radio-magnetotelluric data
Authors: Rulff Paula , Weckmann Ute , Kalscheuer Thomas , Willkommen Gregor , Buntin Laura Maria , Platz Anna
Published: July 2024

The pathways of fluids and mantle originated carbon dioxide in the seismically active Ohře (Eger) Rift system appearing as mofettes at the surface are currently subject to investigation, especially by the International Continental Scientific Drilling Program “Drilling the Eger Rift”. If the aquifers show significant contrast in electrical resistivity to the host rocks, they can be investigated with geo-electromagnetic methods. However, imaging complex fluid and CO2 pathways in detail in near-surface structures is challenging, because, in contrast to the background stratigraphy, they are often oriented in near-vertical directions. Therefore, we aim to investigate how the shallow aquifer structures can be examined best with an inductive electromagnetic method. For this purpose, we collected radio-magnetotelluric data in the Hartoušov mofette field and evaluated them by two- and three-dimensional inversions. Data of a nearby magnetotelluric station, drill hole data, gas flux measurements and electrical resistivity tomography models were used to assess reliability and robustness of our inversion results. We concluded that the near-surface fluid reservoirs are adequately depictable, while the migration paths of gaseous CO2 cannot be traced properly due to a lack of resistivity contrast. Our model analyses suggest that imaging the given geological setting with fluids and gases ascending in anastomosing pathways, benefits from a fine-scale three-dimensional inversion approach, because the fluids mostly appear as local conductive reservoir-like anomalies, which can be falsely projected onto the profiles during inversion in two dimensions. The resistivity models contribute with detailed images of the near-surface aquifers to the geodynamic model of the Ohře Rift.
Backup Subscription Scheme for differential protection using IEC61850-9-2 Sampled Values
Authors: Katoulaei mohammad Khalili , Rahmani Aamir , Høidalen Hans Kristian , Oleinikova Irina , Mork Bruce
Published: July 2024

In IEC-61850-based digital substations, the protection IED's performance is dependent on merging unit's vendor implementation, communication networks, and measurement circuit's health conditions. As the process bus Sampled Value(SV) enables the availability of all sensor data on a communication network, this paper proposes a Backup Subscription scheme (BSS) for a transformer differential protection (87T, PDIF) function. BSS utilizes sensor data in digital substations to achieve a flexible protection scheme using a dynamic subscription feature. Thus, in case of failure of one sensor, differential protection would be maintained. The paper presents the implementation and verification of a prototype scheme using a hardware-in-the-loop simulation test bed. The main result is that integration of BSS into differential protection ensures its dependability and security. Moreover, delay compensation and seamless switching feature increases the availability of differential protection.
Single Photon Event-Driven 3D Imaging
Authors: Kirkland Paul , Sola Alex Vicente , Aquilina Matthias , Lyons Ashley
Published: July 2024

Event-based imaging is at the forefront of high-speed sensing applications due to the low latency of asynchronous detection and low data volume. Conventionally, events used in this form of sensing correspond to changes in intensity on the microsecond scale, this inherently makes the approach incompatible with scenarios where single photon detection is required such as single photon Light Detection and Ranging (LiDAR), and low-light-level imaging. We propose a new imaging modality which is driven instead by events generated from the detection of individual photons. We use a form of single-pixel imaging in which information from a 3-Dimensional scene is encoded entirely in the time-of-arrival of the photons such that each detected photon can be used to update an estimation of all transverse positions simultaneously. The image reconstruction is performed by a Spiking Convolutional Neural Network (SCNN) which has a natural complementarity with single photon detection that allows the scheme to run fully asynchronously and be driven by the detection of each individual photon.
Phylogenomic analysis of African swine fever virus isolates from the Philippines
Authors: Ko Szarina Krisha K. , Navarro Rohani C. , Galarion Ma Jowina H. , Janairo Gabriela Ilona B. , Abarientos Adrian B. , Azul Rachel R. , Legaspi Cristina F. , Maravilla Jennifer L. , Schwem Brian E.
Published: July 2024

African swine fever (ASF) is a re-emerging viral disease of swine, causing highly contagious hemorrhagic infection and devastating mortality in domestic pigs. It was first reported in the Philippines in July 2019 and outbreaks have since spread nationwide, reportedly reducing 30% of the local swine population. The present study aims to analyze the phylogenomic characteristics of two ASF virus isolates collected from outbreaks in the Philippines in May and June of 2020. Downstream analysis of short read metagenomic Illumina sequencing data generated two near full-length genome sequences of ASF viruses of genotype 2, serogroup 8, CVR 1, and IGR II. The current study identified 33 genetic variations within the Philippine isolates relative to ASFV Georgia 2007/1.
Exploring health equity integration among health service and delivery systems in Nova Scotia: Perspectives of health system partners
Authors: Yusuf Joshua , D’Souza Ninoshka J. , Caldwell Hilary A.T. , Sim Sarah Meaghan , Embrett Mark , Kirk Sara F.L.
Published: July 2024

Background
Achieving health equity is important to improve population health; however, health equity is not typically well defined, integrated, or measured within health service and delivery systems. To improve population health provincially, it is necessary to understand barriers and facilitators to health equity integration within health service and delivery systems. This study aimed to explore health equity integration among health systems workers and identify key barriers and facilitators to implementing health equity strategies within the health service and delivery system in Nova Scotia, ahead of the release of a Health Equity Framework, focused on addressing inequities within publicly funded institutions.
Methods
Purposive sampling was used to recruit individuals working on health equity initiatives including those in high-level leadership positions within the Nova Scotia health system. Individual interviews and a focus group session were conducted. Topics of discussion included current integration of health equity through existing strategies and perceptions within participant roles. The Consolidated Framework for Implementation Research was used to guide coding and analysis, with interviews transcribed and deductively analyzed in NVivo. Qualitative description was employed to describe study findings as barriers and facilitators to health equity integration.
Results
Eleven individual interviews and one focus group (n = 5 participants) were conducted, with a total of 16 participants. Half (n = 8) of the participants were High-level Leaders (i.e., manager or higher) within the health system. We found that the CFIR subdomains of Complexity, Innovation Design, Mission Alignment, Available Resources and Roles: Mid and High-level leaders were barriers to health equity integration. The perceived homogeneity of leadership and therefore their capability to understand health equity accounted for the identification of leaders as a barrier. Facilitators for health equity integration included the subdomains Partnerships and Connections, Policies and Laws, and Engagement as a multi-domain facilitator. Existing strategies within the health system were found to be inadequate to address inequities.
Conclusion
Our findings suggest that health equity integration needs to be prioritized within the health service and delivery system within Nova Scotia and identifies possible strategies for implementation. Appropriate measures and training resources need to be put in place to support health equity integration.
Surface temperature characteristics of patients with malignant lymphoma based on infrared thermal imaging technology
Authors: Wu Junfan , Jia Qiuran , li dongyun , Zhang Wenzheng
Published: July 2024

Objective: To evaluate the difference in body surface temperature characteristics between patients with malignant lymphoma and healthy people by infrared thermography, and to find the independent risk factors for malignant lymphoma in temperature characteristics.
Methods: The infrared heat maps of 40 patients with malignant lymphoma and 40 healthy people who were admitted to Dongzhimen Hospital from December 2022 to December 2023 were collected, and the temperature characteristics of the target area were measured and analyzed.
Results: The average temperature of hands and feet in the malignant lymphoma group was higher than that in the normal control group. The average temperature of the abdomen, spine and back of the control group was lower than that of the normal control group (P < 0.05). The homogeneity of the neck and abdomen was worse than that of the normal control group (P < 0.05). The symmetry of the anterior, neck and clavicle region was worse than that of the normal control group (P < 0.05). Lower abdominal homogeneity and neck symmetry were independent risk factors for the diagnosis of malignant lymphoma (P<0.05).
Conclusion: Infrared thermal imaging is beneficial to the screening of malignant lymphoma and the evaluation of therapeutic effect.
Mechanical interactions between tissue layers underlie plant morphogenesis
Authors: Kierzkowski Daniel , Silveira Sylvia , Collet Loann , Haque Sahil , Lapierre Luc , Bagniewska-Zadworna Agnieszka , Gosselin Frederick , Smith Richard , Routier-Kierzkowska Anne-Lise
Published: July 2024

Differential growth between tissues generates mechanical conflicts regulating organogenesis in plants. Here, we use the anther – floral male reproductive organ – as a model system to understand how cell dynamics and tissue-scale mechanics control 3D morphogenesis of a complex shape. Combining deep live cell imaging, growth analysis, osmotic treatments, genetics, and mechanical modeling, we show that localized expansion of internal cells actively drives anther lobes outgrowth, while the epidermis passively stretches. At later stages mechanical load is transferred to the subepidermal layer (endothecium), contributing to proper organ shape. We propose the concept of inflation potential, encapsulating mechanical and anatomical features causing differential growth. Our data emphasizes the active mechanical role of inner tissue in controlling both the organ shape acquisition and cell dynamics in outer layers.
Visceral Adipose Tissue, Epicardial Fat, and Hepatic Steatosis in Polycystic Ovary Syndrome: A Study of Ectopic Fat Stores and Metabolic Dysfunction
Authors: Cavalcante Rebeca Bandeira de Melo , Leão Lenora Maria Camarate Silveira Martins , Tavares Ana Beatriz Winter , Lopes Karynne Grutter , Terra Carlos , Salgado Angelo Antunes , Kraemer-Aguiar Luiz Guilherme
Published: July 2024

Purpose: Ectopic fat accumulation remains a topic of debate in polycystic ovary syndrome (PCOS). Therefore, intra-abdominal, hepatic, and epicardial fat were compared between PCOS women and body mass index (BMI)-matched controls, and their associations with metabolic and hormonal parameters were explored. Furthermore, the performance of echocardiographic epicardial adipose tissue thickness (EATT) and hepatic steatosis measurement by transient elastography-based controlled attenuation parameter (TE-CAP) in screening abdominal visceral adipose tissue (VAT) was evaluated.
Methods: Women aged 18–39 years with BMI < 35 kg/m² were recruited. PCOS was defined by the Rotterdam criteria. All participants underwent clinical and laboratory exams, dual-energy X-ray absorptiometry (DXA), TE-CAP, and echocardiography. A receiver operating characteristic curve was applied to evaluate accuracy and optimal cutoff values of TE-CAP and EATT in predicting DXA-measured VAT.
Results: The study included 72 volunteers (35 with PCOS, 37 controls). PCOS women exhibited higher levels of androgens, insulin resistance (IR) parameters, LDL-cholesterol, triglycerides, VAT, and EATT. VAT correlated with IR and triglycerides, whereas EATT correlated with HDL-cholesterol. In PCOS women aged 18–29 years, the cutoff values of CAP and EATT for VAT were 198.0 and 3.07, respectively, with CAP demonstrating higher AUC. In PCOS women aged 30–39 years, the cutoff values were 209.5 and 3.36, respectively, with EATT showing higher AUC.
Conclusion: VAT correlates with more metabolic parameters in PCOS than TE-CAP or EATT. TE-CAP is useful for VAT screening in PCOS patients aged 18–39 years, whereas EATT is effective and outperforms CAP in those aged 30–39 years.
Managment of ipsilateral interstitial pregnancy after tubal surgery: a retrospective study focusing on focal rupture
Authors: Jing Miaomiao , Zhao Wei
Published: July 2024

Study Objective：To explore the high risk factors and clinical characteristics of interstitial pregnancy rupture by retrospectively studying the clinical characteristics, diagnosis, treatment and prognosis of patients with interstitial pregnancy, so as to provide a certain reference and basis for the clinical management of interstitial pregnancy.Design: A single-center, retrospective study.Setting: A university-based hospital.Patients: Patients diagnosed with interstitial pregnancy in the Women’s Hospital, Zhejiang University School of Medicine from January 2009 to May 2022 and treated in the hospital for the first time.Interventions: A retrospective statistical analysis was performed on the case data of patients with interstitial pregnancy who met the inclusion criteria and were treated in the Obstetrics and Gynecology Hospital Affiliated to Zhejiang University Medical College, and statistical software SPSS was used for data analysis.Measurement and Main Results: A total of 885 patients with interstitial pregnancy were included in the study, including 55 patients with heterotopic interstitial pregnancies. The gestational days of patients with interstitial rupture were shorter than those of intactness(p<0.01). The risk of interstitial pregnancy rupture was higher in women with a history of ipsilateral fallopian tube surgeryp<0.01). In the >42day rupture group, the gestation days were shorter in those with a history of ipsilateral surgery than those without, and the difference was statistically significant(p=0.005). The interval between ipsilateral tubal surgery and this interstitial pregnancy was 12 months as the cut-off point for analysis. The shorter the interval, the higher the risk of interstitial pregnancy rupture (p=0.001).Conclusions: Patients with a history of ipsilateral tubal surgery have a higher risk of interstitial pregnancy rupture. The shorter the interval between ipsilateral tubal surgery and interstitial pregnancy, the higher the risk of rupture. For patients with intrauterine pregnancy and interstitial pregnancy, timely treatment can also obtain term live birth.
UNC5B Knockdown Enhances Chemotherapy Sensitivity and Immunogenic Cell Death in Sarcoma: A Comprehensive Prognostic Analysis based on PANoptosis and Macrophage
Authors: Li Xuanxuan , Du Yumeng , Deng Qing , Cong Chuqi , Zhang Yueqiang , Li Yufei , Wang Dong
Published: July 2024

Purpose
Sarcomas represent a heterogeneous collection of malignancies originating from mesodermal tissues, posing significant challenges in diagnosis and treatment. Emerging research has spotlighted PANoptosis—a synergistic process of pyroptosis, apoptosis, and necroptosis—as a novel therapeutic target. This study seeks to construct a prognostic model leveraging PANoptosis and macrophage-related genes to advance the understanding and treatment of sarcoma.
Methods
Utilizing data from the TCGA-SARC, TARGET-OS cohorts, and GSE159847, we identified genes associated with macrophages and PANoptosis. A prognostic model was formulated through multivariate and LASSO regression analyses, with its efficacy evaluated via Kaplan-Meier survival analysis, ROC curves, and validation with an independent cohort. Comprehensive analyses included functional enrichment, immune microenvironment assessment, and drug response prediction. Additionally, functional assays were performed on 143B cells to elucidate the pivotal gene’s roles.
Results
The prognostic model, incorporating four genes (CD2, STAT6, TXNIP, UNC5B), effectively categorized samples into high and low risk cohorts with notable survival disparities. Samples from the high-risk group exhibited greater genomic instability and immunosuppressive microenvironments. Functional assays in 143B cells revealed that knockdown of UNC5B reduced cell proliferation and increased sensitivity to paclitaxel, indicating potential therapeutic targets.
Conclusion
This study highlights the critical role of PANoptosis and macrophage polarization in the sarcoma microenvironment. The prognostic model devised provides a reliable instrument for predicting patient outcomes and tailoring personalized treatment strategies, thereby offering innovative therapeutic pathways for sarcoma management.
Native and exotic vegetation cover shape avocado pollinator communities at the landscape scale
Authors: Young Gemma G , Howlett Brad , Clearwater Michael J , Barnes Andrew
Published: July 2024

Insect pollinators play a crucial role in maintaining biodiversity and ecosystem functioning. However, there are concerning declines in pollinators worldwide, with agricultural conversions and intensification being one of the largest drivers. Therefore, understanding the relationship between features of the landscape and pollinator communities is crucial for managing pollinator biodiversity and associated ecosystem services. We sampled avocado-associated pollinator communities on 24 avocado orchards using flight intercept pan traps. The percentage of vegetation, connectivity, spectral functional diversity and spectral species richness for both native and woody (both native and exotic) vegetation was measured surrounding the avocados at three spatial scales (250 m, 500 m, 1 km). We then estimated the relative influence of these landscape factors on pollinator species richness, evenness, abundance, biomass and mean body size. We found a complex suite of effects of the landscape on avocado pollinators, with multiple variables identified as important for describing variation in pollinator biodiversity. Specifically, spectral plant diversity was shown to decrease pollinator abundance, the connectivity of the woody vegetation decreased pollinator evenness and increasing woody vegetation had a positive effect on pollinator mean body mass. The findings of this study contrast previous studies suggesting that both native and exotic vegetation may be equally important for enhancing pollinator biodiversity. Our results suggest that the most effective approach to increase pollinator diversity, density and body size in avocado orchards is to increase the presence of mixed vegetation hedgerows and improving nearby woody areas.
The Positive Effect of Internet Use on Mental Health in Older Adults A Nationally Representative Cross-Sectional Survey
Authors: zuo xinyi , chen yifang
Published: July 2024

Background
There is growing evidence in Europe such as Germany and American discovered that the internet has beneficial effects on the mental health of older people (aged ≥ 60 years); however, the evidence is inconclusive, and the underlying mechanisms are poorly understood.
Objective
The aim of this cross-sectional survey was to understand the patterns of internet use and measure their associations with the risk of depression in China from two dimensions: the reasons for using the internet use and types of devices used. In addition, this study explored the relationship between internet use and depression risk in elderly people at the individual and provincial levels and in rural and urban subgroups.
Methods
The survey of CHARLS used a multistage probability-proportional-to-size (PPS) sampling technique. Province-level socioeconomic characteristics were merged with microdata for respondents over 60 years of age from the 2020 China Health and Retirement Longitudinal Study (CHARLS 2020) Wave 5 (n = 10,036) by author. The respondents were asked whether they used the internet, their reasons for using the internet and the types of internet devices used. Risk of depression was assessed using the 10-item Center for Epidemiological Studies Depression Scale (CES-D 10). The chi-square test was used to explore whether internet use was associated with mental health status. A binary logistic regression model was used to evaluate this association further after controlling for confounding factors.
Results
Overall, 24.2% (2426/10036) of the participants over 60 years of age reported using the internet. Regression analyses revealed that older adults who used the internet (OR = 0.723, 95% CI: 0.607–0.863, p < .001) had a lower risk of depression than did those who did not use the internet. In addition, older adults who were female (P < .005), were single (P < .001), lived in a rural area (P < .001), had poor self-rated health (SRH) (P < .001), had no retirement pension (P < .001) had a greater risk of depression. For province-level characteristics, older adults who lived in provinces in the first quartile (P < .005) of gross domestic product (GDP) per capita were more likely to experience depressive symptoms while the number of beds in medical institutions per 10,000 persons shows no effect. In the binary logistic regression analysis of the urban and rural subgroups separately, the results are indicated Internet use was negatively associated with risks of depression, especially in rural(P < .001) but not in urban(P = .095) participants. And none of the participants in the rural subgroup reported using a laptop.
Conclusions
Internet use had a positive effect on depression, especially in rural but not in urban participants. And the rate of ownership of internet devices also remains low among older Chinese people in rural area. Therefore, rural-urban disparities should be considered when designing strategies to encourage moderate Internet use.
Subtalar arthroscopy-assisted MIPO technique for the treatment of Sanders type Ⅱ and Ⅲ calcaneal fractures via the sinus tarsi approach: A retrospective study
Authors: Hu Xueliang , Cao Shanshan , Dong Jiangtao
Published: July 2024

Background: To explore the efficacy of internal fixation with Minimally invasive plate osteosynthesis (MIPO) technology through the sinus tarsi approach assisted by subtalar arthroscopy in the treatment of Sanders type Ⅱand Ⅲ calcaneal fractures.
Methods: A retrospective analysis was conducted on the data of 55 patients with Sanders type II and III calcaneal fractures admitted to the Cangzhou Clinical College of Integrated Traditional Chinese and Western Medicine of Hebei Medical University from January 2023 to March 2024. The patients were divided into three groups based on different treatment methods: subtalar arthroscopy-assisted (MIPO technology) internal fixation treatment through the sinus tarsi approach (arthroscopic group, 17 patients), the sinus tarsi approach (STA group, 20 patients), and the extensile lateral approach (ELA group, 18 patients). This study compared the preoperative waiting time for swelling reduction, surgical operation time, incidence of incision complications, fracture reduction degree, follow-up time, and surgical score among three groups of patients.
Results:There were no statistically significant differences in the preoperative general information among the three groups of patients, making them comparable. In terms of surgical operation time, X-ray findings, and differences in dorsiflexion and plantarflexion, no significant differences were observed among the groups. The incidence of incision complications, postoperative heel widening compared to that on the contralateral side, and differences in the varus and valgus angles were lower in the arthroscopy group than in the STA group and significantly lower in the STA group than in the ELA group. The visual analogue scale (VAS) score of the postoperative arthroscopy group was lower than that of the other two groups, while the American Orthopedic Foot and Ankle Society (AOFAS) score was greater in the arthroscopy group than in the other two groups.
Conclusion: Subtalar arthroscopy-assisted MIPO technology for internal fixation using the sinus tarsi approach is a promising method for treating Sanders type II and III fractures. This approach has benefits such as less trauma, early treatment, and effective fracture reduction. It is particularly advantageous for young adults or athletes because it minimizes incision complications and reduces tissue scarring.
A bilateral fetal origin of the posterior cerebral artery coexisting with an absent A1 segment of the anterior cerebral artery: A rare combination of variants with critical clinical consequences
Authors: Triantafyllou George , Melissanidis Savvas , Papanagiotou Panagiotis , Tsakotos George , Kalamatianos Theodosis , Vassiou Katerina , Piagkou Maria
Published: July 2024

The cerebral arterial circle variations are well-described due to their clinical significance for neurosurgeons and neuroradiologists. In this imaging report, we describe the unusual coexistence of three cerebral variants incidentally identified during magnetic resonance angiography (MRA) of a 44-year-old female patient. The right-sided first segment (A1) of the anterior cerebral artery (ACA) was absent, and the posterior cerebral arteries (PCAs) were bilaterally originating from the internal carotid arteries (a fetal-type origin). The coexistence of these variants is rare, and they significantly disrupt the patient’s primary collateral pathway. The clinical significance and consequences of such variants after stroke or transient ischemic attack cannot be overstated, underscoring the importance of the current imaging finding in understanding and managing these conditions.
Construction of magnetic Ag3PO4/Fe3O4/Chitosan polymer composite with enhanced visible-light-driven photocatalytic activity for the Methylene blue dye degradation
Authors: Somasundaram Annette Jayam , Elanthamilan Elaiyappillai , Wang Sea-Fue , Lydia I. Sharmila
Published: July 2024

The present study employed the simple co-precipitation approach followed by ultrasonication to generate a composite material consisting of magnetic Ag3PO4/Fe3O4/Chitosan (CS). The magnetic Ag3PO4/Fe3O4/CS composite was characterized using the Fourier transform infrared (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), and X-ray photoelectron spectroscopy (XPS) methods. The magnetic composite was evaluated as a photocatalyst for methylene blue (MB) degradation under visible light irradiation. The investigation also focused on optimizing the photocatalyst dose, concentration fluctuation, and stability to enhance the reaction conditions for dye degradation. The magnetic Ag3PO4/Fe3O4/CS composite exhibited robust photocatalytic activity for the degradation of MB, with a removal efficiency of 95%. Furthermore, the composite demonstrated exceptional recyclability and reusability while experiencing no degradation in catalytic activity. The results of this study will aid in the advancement of environmentally friendly nanotechnology by enabling the production of easily separable magnetic Ag3PO4/Fe3O4/CS composite as heterogeneous catalysts.
Live cell protein imaging of tandem complemented-GFP11-tagged Coiled-coil domain-containing protein-124 identifies this factor in G3BP1-induced stress-granules.
Authors: Hacıbeyoğlu Kübra , Öztürk Merve Tuzlakoğlu , Arslan Özge , Tazebay Uygar Halis
Published: July 2024

Coiled-coil domain-containing 124 protein is a multifunctional RNA-binding factor, and it was previously reported to interact with various biomolecular complexes localized at diverse subcellular locations, such as the ribosome, centrosome, midbody, and nucleoli. We aimed to better characterize the subcellular CCDC124 translocation by labelling this protein with a fluorescent tag, followed by laser scanning confocal microscopy methods. As traditional GFP-tagging of small proteins such as CCDC124 often faces limitations like potential structural perturbations of labeled proteins, and interference of the fluorescent-tag with their endogenous cellular functions, we aimed to label CCDC124 with the smallest possible split-GFP associated protein-tagging system (GFP11/GFP1-10) for better characterization of its subcellular localizations and its translocation dynamics. By recombinant DNA techniques we generated CCDC124-constructs labelled with either single of four tandem copies of GFP11 (GFP11x1::CCDC124, GFP11x4::CCDC124, or CCDC124::GFP11x4). We then cotransfected U2OS cells with these split-GFP constructs (GFP11x1(or X4)::CCDC124/GFP1-10) and analyzed subcellular localization of CCDC124 protein by laser scanning confocal microscopy. Tagging CCDC124 with four tandem copies of a 16-amino acid short GFP-derived peptide-tag (GFP11X4::CCDC124) allowed better characterization of the subcellular localization of CCDC124 protein in our model human bone osteosarcoma (U2OS) cells. Thus, by this novel methodology we successfully identified GFP11x4::CCDC124 molecules in G3BP1-overexpression induced stress-granules by live cell protein imaging for the first time. Our findings propose CCDC124 as a novel component of the stress granule which is a membraneless organelle involved in translational shut-down in response to cellular stress.