bluuebunny/crossref_metadata_2025 huggingface dataset:
Complex ALK Fusions Are Associated With Better Prognosis in Advanced Non-Small Cell Lung Cancer
Authors: Kang Jin , Zhang Xu-Chao , Chen Hua-Jun , Zhong Wen-Zhao , Xu Yang , Su Jian , Zhou Qing , Tu Hai-Yan , Wang Zhen , Xu Chong-Rui , Yang Xue-Ning , Chen Zhi-Hong , Wu Xue , Zhang Xian , Shao Yang , Wu Yi-Long , Yang Jin-Ji
Published: December 2020

Background

Echinoderm microtubule-associated protein-like 4 (EML4) is the canonical anaplastic lymphoma kinase (ALK) fusion partner in non-small cell lung cancer (NSCLC), and ALK-positive patients showed promising responses to ALK tyrosine kinase inhibitors (TKIs). However, studies that comprehensively investigate ALK TKI treatment in patients with different ALK fusion patterns are still lacking.

Methods

Ninety-eight ALK-positive patients with advanced NSCLC were retrospectively studied for their response to crizotinib and subsequent treatments. Comprehensive genomic profiling (CGP) was conducted to divide patients into different groups based on their ALK fusion patterns. Non-canonical ALK fusions were validated using RNA-sequencing.

Results

54.1% of patients had pure canonical EML4-ALK fusions, 19.4% carried only non-canonical ALK fusions, and 26.5% harbored complex ALK fusions with coexisting canonical and non-canonical ALK fusions. The objective response rate and median progression-free survival to crizotinib treatment tended to be better in the complex ALK fusion group. Notably, patients with complex ALK fusions had significantly improved overall survival after crizotinib treatment (p = 0.012), especially when compared with the pure canonical EML4-ALK fusion group (p = 0.010). The complex ALK fusion group also tended to respond better to next-generation ALK TKIs, which were used as later-line therapies. Most identified non-canonical ALK fusions were likely to be expressed in tumors, and some of them formed canonical EML4-ALK transcripts during mRNA maturation.

Conclusion

Our results suggest NSCLC patients with complex ALK fusions could potentially have better treatment outcomes to ALK TKIs therapy. Also, diagnosis using CGP is of great value to identify novel ALK fusions and predict prognosis.
Melatonin Attenuates Sepsis-Induced Acute Lung Injury Through Improvement of Epithelial Sodium Channel-Mediated Alveolar Fluid Clearance Via Activation of SIRT1/SGK1/Nedd4-2 Signaling Pathway
Authors: Li Jing , Liu Longfei , Zhou Xiaojun , Lu Xianzhou , Liu Xianrong , Li Guojuan , Long Jianwu
Published: December 2020

Acute lung injury is characterized by alveolar vascular barrier injury, and protein-rich pulmonary oedema. Alveolar fluid clearance is closely related to the prognosis of patients with acute lung injury. Melatonin has been shown to have a protective effect on multiple organ injury induced by sepsis. In this study we investigated the effect of melatonin on alveolar fluid clearance (AFC) and explored its potential mechanisms in sepsis-induced acute lung injury. The cecal ligation and puncture was adopted to establish mouse sepsis model. Morphological changes of lung tissues with the hematoxylin staining were observed. AFC and lung wet/dry weight ratio were measured to assess pulmonary edema. Inflammatory mediators in bronchoalveolar lavage fluid were analyzed via enzyme-linked immunosorbent assay. NAD+/NADH and SIRT1 activity were measured by colorimetric assay kit. The protein expressions of epithelial sodium channel (ENaC), silent information regulator1 (SIRT1), SGK1 and Nedd4-2 were immunoblotted by western blot in vivo and in vitro. The distribution of α-ENaC and SIRT1 was detected by immunofluorescence. We found that melatonin attenuated sepsis induced lung injury, improved survival rate, enhanced alveolar fluid clearance, improved SIRT1 activity, increased protein expressions of SIRT1 and ENaC, and activated SGK1/Nedd4-2 pathway. Furthermore, SIRT1 inhibitor EX527 counteracted the effects of melatonin on alveolar fluid clearance and ENaC. These results revealed that melatonin enhanced ENaC-mediated AFC via the SIRT1/SGK1/Nedd4-2 signaling pathway. Our study demonstrated that melatonin might provide a novel therapeutic strategy for sepsis-induced acute lung injury.
Innsbruck Teleportation Experiment in the Wigner Formalism: A Realistic Description Based on the Role of the Zero-Point Field
Authors: Casado Alberto , Guerra Santiago , Plácido José
Published: December 2020

In this article, an undulatory description of the Innsbruck teleportation experiment is given, grounded in the role of the zero-point field (ZPF). The Wigner approach in the Heisenberg picture is used, so that the quadruple correlations of the field, along with the subtraction of the zero-point intensity at the detectors, are shown to be the essential ingredients that replace entanglement and collapse. This study contrasts sharply with the standard particle-like analysis and offers the possibility of understanding the hidden mechanism of teleportation, relying on vacuum amplitudes as hidden variables.
Spreading Kinetics of Herschel-Bulkley Fluids Over Solid Substrates
Authors: Zhang Jie , Gu Hai , Sun Jianhua , Li Bin , Jiang Jie , Wu Weiwei
Published: December 2020

The spreading kinetics of Herschel-Bulkley fluids on horizontal solid substrates were theoretically studied. The equations of film thickness were derived in both gravitational and capillary regimes. The dynamic contact angle for the capillary regime was also derived. Finally, a limiting result for the case of τ0 = 0 was obtained, which was compared with the known solution for validation. The results show that the yield behavior of the fluids had a significant impact on the spreading kinetics in both cases. Only when stress was larger than the yield stress, would substantial flow occur. The spreading zone was divided into two parts by the yield surface: sheared zone and yield zone, which was completely different from common Newtonian fluids or power-law fluids. The thickness of the yield zone mainly depended on yield stress and pressure gradient along the z-direction. According to the final evolution, both the film thickness and dynamic contact angle were affected not only by the power-law index but also by the yield behavior.
The Effects of Sports Drinks During High-Intensity Exercise on the Carbohydrate Oxidation Rate Among Athletes: A Systematic Review and Meta-Analysis
Authors: Li Xudong , Wang Wanxia , Guo Rui , Wang Anqi , Wei Chaojun
Published: December 2020

Background: This study examines the effects of sports drinks ingestion during high-intensity exercise for carbohydrate oxidation rate (CHO-O) among athletes.

Methods: PubMed, Embase, and the Cochrane library were searched for available papers published up to November 2019. The primary outcome is the carbohydrate oxidation rate (CHO-O), and the secondary outcome is the fat oxidation rate (Fat-O). Statistical heterogeneity among the included studies was evaluated using Cochran's Q test and the I2 index. The random-effects model was used for all analyses, regardless of the I2 index.

Results: Five studies are included, with a total of 58 participants (range, 8–14/study). All five studies are randomized crossover trials. Compared to the control beverages, sports drinks have no impact on the CHO-O of athletes [weighted mean difference (WMD) = 0.29; 95% CI, −0.06 to 0.65, P = 0.106; I2 = 97.4%, P < 0.001] and on the Fat-O of athletes (WMD = −0.074; 95% CI, −0.19 to 0.06, P = 0.297; I2 = 97.5%, P < 0.001). Carbohydrate–electrolyte solutions increase CHO-O (WMD = 0.47; 95% CI, 0.08–0.87, P = 0.020; I2 = 97.8%, P < 0.001) but not Fat-O (WMD = −0.14; 95% CI, −0.31 to 0.03, P = 0.103; I2 = 98.2%, P < 0.001). Caffeine has a borderline effect on Fat-O (WMD = 0.05; 95% CI, 0.00–0.10, P = 0.050).

Conclusions: Compared with the control beverages, sports drinks show no significant improvement in CHO-O and Fat-O in athletes. Carbohydrate–electrolyte solutions increase CHO-O in athletes but not Fat-O.
Trait Disinhibition and NoGo Event-Related Potentials in Violent Mentally Disordered Offenders and Healthy Controls
Authors: Delfin Carl , Ruzich Emily , Wallinius Märta , Björnsdotter Malin , Andiné Peter
Published: December 2020

Trait disinhibition may function as a dispositional liability toward maladaptive behaviors relevant in the treatment of mentally disordered offenders (MDOs). Reduced amplitude and prolonged latency of the NoGo N2 and P3 event-related potentials have emerged as promising candidates for transdiagnostic, biobehavioral markers of trait disinhibition, yet no study has specifically investigated these two components in violent, inpatient MDOs. Here, we examined self-reported trait disinhibition, experimentally assessed response inhibition, and NoGo N2 and P3 amplitude and latency in male, violent MDOs (N = 27) and healthy controls (N = 20). MDOs had a higher degree of trait disinhibition, reduced NoGo P3 amplitude, and delayed NoGo P3 latency compared to controls. The reduced NoGo P3 amplitude and delayed NoGo P3 latency in MDOs may stem from deficits during monitoring or evaluation of behavior. NoGo P3 latency was associated with increased trait disinhibition in the whole sample, suggesting that trait disinhibition may be associated with reduced neural efficiency during later stages of outcome monitoring or evaluation. Findings for NoGo N2 amplitude and latency were small and non-robust. With several limitations in mind, this is the first study to demonstrate attenuated NoGo P3 amplitude and delayed NoGo P3 latency in violent, inpatient MDOs compared to healthy controls.
Community-Based Family Workshop Intervention Improved the Social Adaptation of Left-Behind Children in Rural China
Authors: Zhang Xiu , Li Mengjie , Guo Li , Zhu Yanna
Published: December 2020

Purpose: With the rapidly developing economy in China, there are more than 50 million left-behind children (LBC) in rural China, whose social adaptation has become a public concern. Thus, the current study aimed to investigate the effect of community-based family workshop intervention on social adaptation among rural LBC in China.

Methods: A cluster randomized trial was conducted with 104 LBC, in which 66 LBC-caregivers dyads received guidance from community-based family workshop for 6 months, while the controls (LBC n = 38) received routinely parenting guidance. Social adaptation of the children was assessed by the Strength and Difficulties Questionnaire (SDQ) at the beginning and the end of the intervention.

Findings: Compared with controls, results showed remarkable improvement on emotional symptoms (P = 0.050), peer problems (P = 0.050), and total difficulties score (TDS, P = 0.040) in the intervention group, especially those aged 3–6 years. Moreover, SDQ score of TDS (P = 0.039), peer problems (P = 0.013), and hyperactivity–inattention (P = 0.023) decreased after intervention in boys aged 3–6 years, while emotional symptoms (P = 0.048) in girls. Finally, improvement on peer problems (P = 0.005) was observed in participants with high TDS.

Conclusions: The findings suggest that community-based family workshop intervention can improve social adaptation of rural LBC. Moreover, this effect was different in boys and girls and also affected by age and baseline total difficulties. The results indicated that community-based family workshop intervention can be implemented in rural China to improve mental and behavioral health among LBC in the future.
MANTRA: Improving Knowledge of Maternal Health, Neonatal Health, and Geohazards in Women in Rural Nepal Using a Mobile Serious Game
Authors: Mueller Sonja , Soriano Delphine , Boscor Andrei , Saville Naomi M. , Arjyal Abriti , Baral Sushil , Fordham Maureen , Hearn Gareth , Kayastha Rachya , Kostkova Patty
Published: December 2020

Serious games, conveying educational knowledge rather than merely entertainment, are a rapidly expanding research domain for cutting-edge educational technology. Digital interventions like serious games are great opportunities to overcome challenges in low-and-middle-income countries that limit access to health information, such as social barriers like low-literacy and gender. MANTRA: Increasing maternal and child health resilience before, during and after disasters using mobile technology in Nepal takes on these challenges with a novel digital health intervention; a serious mobile game aimed at vulnerable low-literacy female audiences in rural Nepal. The serious game teaches 28 learning objectives of danger signs in geohazards, maternal, and neonatal health to improve knowledge and self-assessment of common conditions and risks to inform healthcare-seeking behavior. Evaluations consisted of recruiting 35 end users to participate in a pre-test assessment, playing the game, post-test assessment, and focus groups to elicit qualitative feedback. Assessments analyzed knowledge gain in two ways; by learning objective with McNemar tests for each learning objective, and by participant scores with paired t-tests of overall scores and by module. Results of assessments of knowledge gain by learning objective (McNemar tests) indicate participants had sufficient prior knowledge to correctly interpret and respond to 26% of pictograms (coded AA), which is a desirable result although without the possibility of improvement through the intervention. The geohazard module had greatest impact as 16% of responses showed knowledge gain (coded BA). The two most successful learning objectives showing statistically significant positive change were evidence of rockfalls and small cracks in the ground (p = < 0.05). Assessment of knowledge gain by participant scores (paired t-tests) showed the 35 participants averaged a 7.7 point improvement (p < 0.001) in the assessment (28 learning objectives). Average change in knowledge of subdivided module scores (each module normalized to 100 points for comparison) was greatest in the geohazard module (9.5 points, p < 0.001), then maternal health (7.4 points, p = 0.0067), and neonatal health (6.0 points, p = 0.013). This evaluation demonstrated that carefully designed digital health interventions with pictograms co-authored by experts and users can teach complex health and geohazard situations. Significant knowledge gain was demonstrated for several learning objectives while those with non-significant or negative change will be re-designed to effectively convey information.
The Current Situation and Future Trend of Leukemia Mortality by Sex and Area in China
Authors: Li Baojing , Tang Hong , Cheng Zilu , Zhang Yuxiao , Xiang Hao
Published: December 2020

Leukemia is one of the most common cancers. We conducted this study to comprehensively analyze the temporal trends of leukemia mortality during 2003–2017 and project the trends until 2030. We extracted national-level data on annual leukemia mortality from China Health Statistics Yearbooks (2003–2017). We applied the Joinpoint regression model to assess leukemia mortality trends in urban and rural China by sex during 2003–2017. We also produced sex-specific leukemia mortality using the adjusted Global Burden Disease (GBD) 2016 projection model. In urban areas, age-standardized leukemia mortality decreased significantly among females during 2003–2017 (APC = −0.9%; 95% CI: −1.7, −0.1%). In rural areas, significant decreases of age-standardized leukemia mortality were both found among males (APC = −1.7%; 95% CI: −2.9, −0.5%) and females (APC = −1.6%; 95% CI: −2.6, −0.7%) from 2008 to 2017. Rural-urban and sex disparities of leukemia mortality will continue to exist until the year 2030. According to projection, the leukemia mortality rates of males and rural populations are higher than that of females and urban populations. In 2030, leukemia mortality is projected to decrease to 3.03/100,000 and 3.33/100,000 among the males in urban and rural areas, respectively. In females, leukemia mortality will decrease to 1.87/100,000 and 2.26/100,000 among urban and rural areas, respectively. Our study suggests that more precautionary measures to reduce leukemia mortality are need, and more attention should be paid to rural residents and males in primary prevention of leukemia in China.
Eating Healthy Might Help the Immune System Fight Cancer
Authors: Huynhle Marvin , Kaur Kawaljit
Published: December 2020

Obesity has been known for years to be a major health problem. Rates of obesity have been steadily increasing all over the world. Many factors, including healthy eating habits and exercise, play important roles in controlling obesity. In our study, we compared the function of cells of the immune system called natural killer (NK) cells between healthy and obese groups of mice. We found that obese mice have lower numbers of NK cells and that NK cells from obese mice are less functional. Lower NK cell activity is related to a higher risk of infections and cancer in the obese group. This research could show a relationship between what we eat and our ability to defend ourselves against diseases like cancer.
New Diagnostic Assays for Differential Diagnosis Between the Two Distinct Lineages of Bovine Influenza D Viruses and Human Influenza C Viruses
Authors: Okda Faten A. , Griffith Elizabeth , Sakr Ahmed , Nelson Eric , Webby Richard
Published: December 2020

Influenza D virus (IDV), a novel orthomyxovirus, is currently emerging in cattle worldwide. It shares >50% sequence similarity with the human influenza C virus (HICV). Two clades of IDV are currently co-circulating in cattle herds in the U.S. New assays specific for each lineage are needed for accurate surveillance. Also, differential diagnosis between zoonotic human influenza C virus and the two clades of IDV are important to assess the zoonotic potential of IDV. We developed an enzyme-linked immunosorbent assay (ELISA) based on two different epitopes HEF and NP and four peptides, and fluorescent focus neutralization assay to differentiate between IDV bovine and swine clades. Calf sera were obtained, and bovine samples underwent surveillance. Our results highlight the importance of position 215 with 212 in determining the heterogeneity between the two lineages. We needed IFA and FFN for tissue culture–based analysis and a BSL2 facility for analyzing virus interactions. Unfortunately, these are not available in many veterinary centers. Hence, our second aim was to develop an iELISA using specific epitopes to detect two lineages of IDVs simultaneously. Epitope-iELISA accurately detects neutralizing and non-neutralizing antibodies against the IDV in non-BSL2 laboratories and veterinary clinics and is cost-effective and sensitive. To differentiate between IDVs and HICVs, whole antigen blocking, polypeptides, and single-peptide ELISAs were developed. A panel of ferret sera against both viruses was used. Results suggested that both IDV and ICV had a common ancestor, and IDV poses a zoonotic risk to individuals with prior or current exposure to cattle. IDV peptides IANAGVK (286–292 aa), KTDSGR (423–428 aa), and RTLTPAT (448–455 aa) could differentiate between the two viruses, whereas peptide AESSVNPGAKPQV (203–215 aa) detected the presence of IDV in human sera but could not deny that it could be ICV, because the only two conserved influenza C peptides shared 52% sequence similarity with IDV and cross-reacted with IDV. However, blocking ELISAs differentiated between the two viruses. Diagnostic tools and assays to differentiate between ICV and IDV are required for serological and epidemiological analysis to clarify the complexity and evolution and eliminate misdiagnosis between ICV and IDV in human samples.
Role of Allergen Immunotherapy in Asthma Treatment and Asthma Development
Authors: Nakagome Kazuyuki , Nagata Makoto
Published: December 2020

Allergen immunotherapy may modify the natural course of allergic diseases and induce remission. It includes subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT). For asthma, allergen immunotherapy using house dust mite (HDM) improves clinical symptoms and airway hyperresponsiveness and decreases drug requirements. Furthermore, it has been suggested that allergen immunotherapy also has the following effects: (1) the effect can be maintained for more than a year even if the treatment is terminated, (2) the remission rate of childhood asthma can be increased, (3) new allergen sensitization can be suppressed, and (4) asthma development can be prevented if allergen immunotherapy was performed in the case of pollinosis. Allergen immunotherapy differs from conventional drug therapy, in particular the effect of modifying the natural course of allergic diseases and the effect of controlling complicated allergic diseases such as rhinoconjunctivitis. The general indication for HDM-SCIT in asthma is HDM-sensitized atopic asthma with mild-to-moderate disease and normal respiratory function. HDM allergens should be involved in the pathogenesis of asthma, and a duration of illness of less than 10 years is desirable. HDM-SLIT is available for allergic rhinitis but not for asthma in Japan. However, as the efficacy of SLIT for asthma has been fully proven internationally, SLIT is also applied in asthmatics with complicated allergic rhinitis in Japan.
Investigation on the Gas–Solid Two-Phase Flow in the Interaction between Plane Shock Wave and Quartz Sand Particles
Authors: Peng Xu , Rao Guoning , Li Bin , Wang Shunyao , Chen Wanghua
Published: December 2020

The interaction between a shock wave and solid particles involves complex gas–solid two-phase flow, which is widely used in industrial processes. Theoretical analysis, an experimental test, and simulation were combined to investigate the interaction process between a shock wave and quartz sand particles. The variation of physical parameters of the two phases during the interaction process was considered theoretically. Then, a novel vertical shock tube generator was employed to record the pressure attenuation and dispersion process of solid particles. Finally, the complex gas–solid two-phase flow was simulated based on the computational fluid dynamics method. The results showed that a nonequilibrium state was formed during the interaction process and momentum exchange generated, resulting in a drag force of the shock wave on the particles. The shock intensity obviously attenuated after the shock wave passed through the solid particles, and this part of the energy was work on the solid particles to drive their dispersion. A three-dimensional annular vortex was generated around the solid particles due to the entrainment effect of airflow. Under the shock wave action of 1.47 Ma, the three types of solid particles with average diameters of 2.5, 0.95, and 0.42 mm presented different motion laws. The particles with smaller size were easier to disperse, and the cloud that formed was larger and more uniform.
Connexins in Cancer: Jekyll or Hyde?
Authors: Mulkearns-Hubert Erin E. , Reizes Ofer , Lathia Justin D.
Published: December 2020

The expression, localization, and function of connexins, the protein subunits that comprise gap junctions, are often altered in cancer. In addition to cell–cell coupling through gap junction channels, connexins also form hemichannels that allow communication between the cell and the extracellular space and perform non-junctional intracellular activities. Historically, connexins have been considered tumor suppressors; however, they can also serve tumor-promoting functions in some contexts. Here, we review the literature surrounding connexins in cancer cells in terms of specific connexin functions and propose that connexins function upstream of most, if not all, of the hallmarks of cancer. The development of advanced connexin targeting approaches remains an opportunity for the field to further interrogate the role of connexins in cancer phenotypes, particularly through the use of in vivo models. More specific modulators of connexin function will both help elucidate the functions of connexins in cancer and advance connexin-specific therapies in the clinic.
A Limited and Diverse Set of Suppressor Mutations Restore Function to INX-8 Mutant Hemichannels in the Caenorhabditis elegans Somatic Gonad
Authors: Starich Todd , Greenstein David
Published: December 2020

In Caenorhabditis elegans, gap junctions couple cells of the somatic gonad with the germline to support germ cell proliferation and gametogenesis. A strong loss-of-function mutation (T239I) affects the second extracellular loop (EL2) of the somatic INX-8 hemichannel subunit. These mutant hemichannels form non-functional gap junctions with germline-expressed innexins. We conducted a genetic screen for suppressor mutations that restore germ cell proliferation in the T239I mutant background and isolated seven intragenic mutations, located in diverse domains of INX-8 but not the EL domains. These second-site mutations compensate for the original channel defect to varying degrees, from nearly complete wild-type rescue, to partial rescue of germline proliferation. One suppressor mutation (E350K) supports the innexin cryo-EM structural model that the channel pore opening is surrounded by a cytoplasmic dome. Two suppressor mutations (S9L and I36N) may form leaky channels that support germline proliferation but cause the demise of somatic sheath cells. Phenotypic analyses of three of the suppressors reveal an equivalency in the rescue of germline proliferation and comparable delays in gametogenesis but a graded rescue of fertility. The mutations described here may be useful for elucidating the biochemical pathways that produce the active biomolecules transiting through soma–germline gap junctions.
Difficult and Severe Asthma in Children
Authors: Porcaro Federica , Ullmann Nicola , Allegorico Annalisa , Di Marco Antonio , Cutrera Renato
Published: December 2020

Asthma is the most frequent chronic inflammatory disease of the lower airways affecting children, and it can still be considered a challenge for pediatricians. Although most asthmatic patients are symptom-free with standard treatments, a small percentage of them suffer from uncontrolled persistent asthma. In these children, a multidisciplinary systematic assessment, including comorbidities, treatment-related issues, environmental exposures, and psychosocial factors is needed. The identification of modifiable factors is important to differentiate children with difficult asthma from those with true severe therapy-resistant asthma. Early intervention on modifiable factors for children with difficult asthma allows for better control of asthma without the need for invasive investigation and further escalation of treatment. Otherwise, addressing a correct diagnosis of true severe therapy-resistant asthma avoids diagnostic and therapeutic delays, allowing patients to benefit from using new and advanced biological therapies.
Precise Determination of Liquid Layer Thickness with Downward Annular Two-Phase Gas-Very Viscous Liquid Flow
Authors: Czernek Krystian , Witczak Stanisław
Published: December 2020

The paper presents the characteristics of the original optoelectronic system for measuring the values of hydrodynamics of two-phase downward gas-very viscous liquid flow. The measurement methods and results of the research on selected values describing gas–oil two-phase flow are presented. The study was conducted in vertical pipes with diameters of 12.5, 16, 22, and 54 mm. The research was conducted with the superficial velocities of air jg = 0–29.9 m/s and oil jl = 0–0.254 m/s, which corresponded to the values of gas stream density gg = (0–37.31) kg/(m2s) and of liquid gl = (0.61–226.87) kg/(m2s), in order to determine the influence of air and oil streams on the character of liquid films. The variations in oil viscosity were applied in the range ηl = (0.055–1.517) Pas. The study results that were obtained with optical probes along with computer image analysis system revealed vast research opportunities in terms of the identification of gas–liquid two-phase downward flow structures that were generated as well as the determination of the thickness of liquid film with various level of interfacial surface area undulation. The designed and constructed proprietary measuring system is also useful for testing the liquid layer by determining the parameters of the resulting waves. It is considered that the apparatus system that is presented in the article is the most effective in examining the properties of liquid layers of oil and other liquids with low electrical conductivity and a significant degree of monochromatic light absorption. In view of noninvasive technique of measuring characteristic values of liquid films being formed, the above measuring system is believed to be very useful for industry in the diagnostics of the apparatus employing such flows.
Demand Flexibility Management for Buildings-to-Grid Integration with Uncertain Generation
Authors: Rostampour Vahab , Badings Thom S. , Scherpen Jacquelien M. A.
Published: December 2020

We present a Buildings-to-Grid (BtG) integration framework with intermittent wind-power generation and demand flexibility management provided by buildings. First, we extend the existing BtG models by introducing uncertain wind-power generation and reformulating the interactions between the Transmission System Operator (TSO), Distribution System Operators (DSO), and buildings. We then develop a unified BtG control framework to deal with forecast errors in the wind power, by considering ancillary services from both reserves and demand-side flexibility. The resulting framework is formulated as a finite-horizon stochastic model predictive control (MPC) problem, which is generally hard to solve due to the unknown distribution of the wind-power generation. To overcome this limitation, we present a tractable robust reformulation, together with probabilistic feasibility guarantees. We demonstrate that the proposed demand flexibility management can substitute the traditional reserve scheduling services in power systems with high levels of uncertain generation. Moreover, we show that this change does not jeopardize the stability of the grid or violate thermal comfort constraints of buildings. We finally provide a large-scale Monte Carlo simulation study to confirm the impact of achievements.
Minimization of Cross-Regulation in PV and Battery Connected Multi-Input Multi-Output DC to DC Converter
Authors: Kamaraj Vibha , Chellammal N. , Chokkalingam Bharatiraja , Munda Josiah Lange
Published: December 2020

This paper proposes a digital model predictive controller (DMPC) for a multi-input multi-output (MIMO) DC-DC converter interfaced with renewable energy resources in a hybrid system. Such MIMO systems generally suffer from cross-regulation, which seriously impacts the stability and speed of response of the system. To solve the contemporary issues in a MIMO system, a controller is required to attenuate the cross-regulation. Therefore, this paper proposes a controller, which increases speed of response and maintains stable output by regulating the load voltage independently. The inductor current and the capacitor voltage of the proposed converter are considered as the controlling parameters. With the aid of Forward Euler’s procedure, the future values are computed for the instantaneous values of controlling parameters. Cost function defines the control action by the predicted values that describe the system performance and establish optimal condition at which the output of the system is required. This allows proper switching of the system, thereby helping to regulate the output voltages. Thus, for any variation in load, the DMPC ensures steady switching operation and minimization of cross-regulation. To prove the efficacy of proposed DMPC controller, simulations followed by the experimental results are executed on a hybrid system consisting of dual-input dual-output (DIDO) positive Super-Lift Luo converter (PSLLC) interfaced with photovoltaic renewable energy resource. The results thus obtained are compared with the conventional PID (proportional integrative derivative) controller for validation and prove that the DMPC controller is able to control the cross-regulation effectively.
Integrated Ultrasonication and Microbubble-Assisted Enzymatic Synthesis of Fructooligosaccharides from Brown Sugar
Authors: Chaisuwan Worraprat , Manassa Apisit , Phimolsiripol Yuthana , Jantanasakulwong Kittisak , Chaiyaso Thanongsak , Pathom-aree Wasu , You SangGuan , Seesuriyachan Phisit
Published: December 2020

Fructooligosaccharides (FOS) are considered prebiotics and have been widely used in various food industries as additives. Ultrasonication has been widely used to enhance food processes; however, there are few reports on ultrasound-assisted FOS synthesis. In the present study, FOS were produced from brown sugar using ultrasonication combined with microbubbles, and the production was optimised using a Box-Behnken experimental design. Here we showed that a combination of ultrasonication and microbubbles could boost the enzyme activity by 366%, and the reaction time was shortened by 60%. The reaction time was a significant variable affecting the FOS production. The optimum conditions were 5 min 45 s of ultrasonication and 7 min 19 s of microbubbles with a reaction time of 5 h 40 min. The maximum enzyme activity and total FOS yield were 102.51 ± 4.69 U·mL−1 and 494.89 ± 19.98 mg·g−1 substrate, respectively. In an enlarged production scale up to 5 L, FOS yields were slightly decreased, but the reaction time was decreased to 4 h. Hence, this technique offers a simple and useful tool for enhancing enzyme activity and reducing reaction time. We have developed a pilot technique as a convenient starting point for enhancing enzyme activity of oligosaccharide production from brown sugar.
How Visuals Affect Food Choice
Authors: Vermeir Iris
Published: December 2020

In this Special Issue, we bring together nine original research articles that demonstrate how visual cues affect consumer reactions that drive food decisions [...]
Analysis of Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay: A Comparative Study of Four Isolation Kits
Authors: Sjoqvist Sebastian , Otake Kentaro , Hirozane Yoshihiko
Published: December 2020

There is a lack of reliable biomarkers for disorders of the central nervous system (CNS), and diagnostics still heavily rely on symptoms that are both subjective and difficult to quantify. The cerebrospinal fluid (CSF) is a promising source of biomarkers due to its close connection to the CNS. Extracellular vesicles are actively secreted by cells, and proteomic analysis of CSF extracellular vesicles (EVs) and their molecular composition likely reflects changes in the CNS to a higher extent compared with total CSF, especially in the case of neuroinflammation, which could increase blood–brain barrier permeability and cause an influx of plasma proteins into the CSF. We used proximity extension assay for proteomic analysis due to its high sensitivity. We believe that this methodology could be useful for de novo biomarker discovery for several CNS diseases. We compared four commercially available kits for EV isolation: MagCapture and ExoIntact (based on magnetic beads), EVSecond L70 (size-exclusion chromatography), and exoEasy (membrane affinity). The isolated EVs were characterized by nanoparticle tracking analysis, ELISA (CD63, CD81 and albumin), and proximity extension assay (PEA) using two different panels, each consisting of 92 markers. The exoEasy samples did not pass the built-in quality controls and were excluded from downstream analysis. The number of detectable proteins in the ExoIntact samples was considerably higher (~150% for the cardiovascular III panel and ~320% for the cell regulation panel) compared with other groups. ExoIntact also showed the highest intersample correlation with an average Pearson’s correlation coefficient of 0.991 compared with 0.985 and 0.927 for MagCapture and EVSecond, respectively. The median coefficient of variation was 5%, 8%, and 22% for ExoIntact, MagCapture, and EVSecond, respectively. Comparing total CSF and ExoIntact samples revealed 70 differentially expressed proteins in the cardiovascular III panel and 17 in the cell regulation panel. To our knowledge, this is the first time that CSF EVs were analyzed by PEA. In conclusion, analysis of CSF EVs by PEA is feasible, and different isolation kits give distinct results, with ExoIntact showing the highest number of identified proteins with the lowest variability.
Autoantibodies against Protein Phosphatase Magnesium-Dependent 1A as a Biomarker for Predicting Radiographic Progression in Ankylosing Spondylitis Treated with Anti-Tumor Necrosis Factor Agents
Authors: Lee Jung-Sun , Lee Eun-Ju , Lee Jae-Hyun , Hong Seok-Chan , Lee Chang-Keun , Yoo Bin , Oh Ji-Seon , Lee Sang-Hoon , Kim Tae-Jong , Lee Seung-Hun , Jo Sung-Sin , Yoo Dae-Hyun , Park Ye-Soo , Kim Tae-Hwan , Kim Yong-Gil
Published: December 2020

Background: Patients with ankylosing spondylitis (AS) have increased levels of protein phosphatase magnesium-dependent 1A (PPM1A) and autoantibodies. We evaluated the usefulness of serum anti-PPM1A antibodies as a biomarker for AS. Methods: Serum samples from 58 AS patients were obtained from a multicenter registry prior to the initiation of anti-TNF agents. The serum levels of anti-PPM1A antibodies were measured using ELISA. Spinal radiographic progression was defined as an increase in the modified stoke ankylosing spondylitis spinal score (mSASSS) by ≥2 units or a newly developed syndesmophyte. The role of exogenous PPM1A on bone mineralization was evaluated using primary osteoprogenitors acquired from patients with AS and non-inflammatory controls. Results: The baseline levels of anti-PPM1A antibodies and mSASSS were higher in the radiographic progression group than in the non-progression group. In logistic regression analysis, baseline mSASSS and serum anti-PPM1A antibodies were associated with a higher risk of progression. The level of anti-PPM1A antibodies for predicting progression had an AUC of 0.716 (cut-off value: 43.77 ng/mL). PPM1A stimulation increased matrix mineralization in AS-osteoprogenitors but not in controls. Conclusion: Along with mSASSS, the serum levels of anti-PPM1A antibodies might be useful as a predictor of radiographic progression after treatment with anti-TNF agents.
Antihypertensive Treatment in Kidney Transplant Recipients—A Current Single Center Experience
Authors: Jehn Ulrich , Schütte-Nütgen Katharina , Strauss Markus , Kunert Jan , Pavenstädt Hermann , Thölking Gerold , Suwelack Barbara , Reuter Stefan
Published: December 2020

Arterial hypertension affects the survival of the kidney graft and the cardiovascular morbidity and mortality of the recipient after kidney transplantation (KTx). Thus, antihypertensive treatment is necessary for a vast majority of these patients. Long-term data on antihypertensive drugs and their effects on allograft function after KTx is still limited, and further investigation is required. We retrospectively analyzed a cohort of 854 recipients who received a kidney transplant at our transplant center between 2007 and 2015 with regard to antihypertensive treatment and its influence on graft function and survival. 1-y after KTx, 95.3% patients were treated with antihypertensive therapy. Of these, 38.6% received mono- or dual-drug therapy, 38.0% received three to four drugs and 8.1% were on a regimen of ≥5 drugs. Beta-blockers were the most frequently used antihypertensive agents (68.1%). Neither the use of angiotensin-converting enzyme inhibitor/angiotensin receptor blockers (51.9%) and calcium channel blockers (51.5%), nor the use the use of loop diuretics (38.7%) affected allograft survival. Arterial hypertension and the number of antihypertensive agents were associated with unfavorable allograft outcomes (each p < 0.001). In addition to the well-known risk factors of cold ischemic time and acute rejection episodes, the number of antihypertensive drugs after one year, which reflects the severity of hypertension, is a strong predictor of unfavorable allograft survival.
Levels of Selected Matrix Metalloproteinases—MMP-1, MMP-2 and Fibronectin in the Saliva of Patients Planned for Endodontic Treatment or Surgical Extraction
Authors: Matuszczak Ewa , Cwalina Izabela , Tylicka Marzena , Wawrzyn Katarzyna , Nowosielska Magdalena , Sankiewicz Anna , Ołdak Łukasz , Gorodkiewicz Ewa , Hermanowicz Adam
Published: December 2020

Objectives: Composition of saliva reflects the condition of the oral cavity. The aim of the study: Investigation of the concentrations of MMP-1 (Matrix metalloproteinase-1), MMP-2 (Matrix metalloproteinase-2) and fibronectin in the saliva of patients planned for endodontic treatment or surgical extraction. Material and methods: Seventy-five patients with caries and 14 healthy subjects were included in the study. Subjects were divided into group 1, in which 50 patients were planned for endodontic treatment, and group 2, in which 25 patients were planned for surgical extraction. For the measurements, we used a surface plasmon resonance imaging biosensor. Results: We found higher levels of MMP-1, MMP-2 and fibronectin in the saliva of patients planned for dental treatment than in healthy donors. We found lower concentrations of MMP-2 in subjects planned for surgical extraction, than in patients planned for endodontic treatment; however, there were no such differences in salivary concentrations of MMP-1 and fibronectin. There were no statistically significant differences in MMP-1 concentrations in the saliva before and after any type of dental treatment, but contrary to that, we found a statistically significant decrease in MMP-2 concentrations after endodontic treatment and after surgical extraction. We found a significant rise in the concentrations of fibronectin after surgical extraction but not after endodontic treatment. Conclusions: The concentrations of MMP-1 and MMP-2 in the saliva of our patients with caries were increased in comparison to healthy individuals, but after the treatment—so sanation of the oral cavity—we noted a decrease in matrix metalloproteinases (MMPs) levels. MMPs can be found in gingival crevicular fluid and saliva, carious dentin and plaque. According to our observations, the main source of MMPs in patients with caries is probably carious dentin. Increase in the salivary levels of fibronectin (FN) after surgical extraction may be connected with soft tissue injury caused by surgical extraction. Our results are another example of the fact that higher salivary concentrations of MMP-1, MMP-2 and FN can reflect the health status of the oral cavity in patients with caries.
Short-Term Results of Ocriplasmin versus Prompt Vitrectomy for Macular Hole. Which Performs Better?
Authors: Cacciamani Andrea , Cosimi Pamela , Di Nicola Marta , Ripandelli Guido , Scarinci Fabio
Published: December 2020

In this retrospective study, we compared the anatomical and functional changes in patients with vitreomacular traction associated with macular holes between the following groups: (1) Patients who were treated with a single intravitreal injection of ocriplasmin (the OCRIALONE group); (2) those who failed the ocriplasmin treatment and underwent vitrectomy one month later (the OCRIVIT group); and (3) patients who directly underwent par plana vitrectomy (VITREALONE group). A total of 38 patients, 19 in the OCRIALONE group + OCRIVIT group (seven and 12 patients, respectively) and 19 in the VITREALONE group with focal vitreomacular adhesion associated with macular holes were evaluated with spectral domain optical coherence tomography. Functional examinations included best-corrected visual acuity (BCVA) and microperimetry analysis. Visual function changes were compared between the OCRIALONE group + OCRIVIT group and VITREALONE group up to three months. Furthermore, a subgroup analysis compared the OCRIVIT group and the VITREALONE group. BCVA values and the mean retinal sensitivity showed statistically significant improvement in all groups (p < 0.001). Specifically, the retinal sensitivity values at the end of the follow-up were significantly higher in the OCRIALONE group + OCRIVIT group than in the VITREALONE group. These functional findings were also confirmed when the statistical analysis was conducted between the OCRIVIT group and the VITREALONE group. Although the OCRIALONE group + OCRIVIT group exhibited faster retinal thinning than the VITREALONE group (p = 0.006), the analysis of the OCRIVIT group versus the VITREALONE group did not show any statistically significant difference. The better functional results and similar anatomical findings suggest that ocriplasmin can be used as a first-line treatment, and that prompt pars plana vitrectomy as primary surgery does not provide better outcomes in comparison with pars plana vitrectomy after ocriplasmin injection.
The Impact of COPD in Trends of Urinary Tract Infection Hospitalizations in Spain, 2001–2018: A Population-Based Study Using Administrative Data
Authors: de Miguel-Diez Javier , Albaladejo-Vicente Romana , Palacios-Ceña Domingo , Carabantes-Alarcon David , Zamorano-Leon José Javier , Lopez-Herranz Marta , Lopez-de-Andres Ana
Published: December 2020

(1) Background: To examine trends in incidence and outcomes of urinary tract infections (UTIs) among men and women with or without chronic obstructive pulmonary disease (COPD), and to identify the predictors for in-hospital mortality (IHM). (2) Methods: We included patients (aged ≥40 years) who were hospitalized with UTIs between 2001 and 2018. Data were collected from the Spanish National Hospital Discharge Database. (3) Results: We identified 748,458 UTI hospitalizations, 6.53% with COPD. The UTIs incidence increased over time. It was 1.55 times higher among men COPD patients than among non-COPD men (incidence rate ratio (IRR) 1.55; 95% CI 1.53–1.56). The opposite happened in women with COPD compared to non-COPD women (IRR 0.30; 95% CI 0.28–0.32). IHM was higher in men with COPD than non-COPD men (5.58% vs. 4.47%; p < 0.001) and the same happened in women (5.62% vs. 4.92%; p < 0.001). The risk of dying increased with age and comorbidity, but the urinary catheter was a protective factor among men (OR 0.75; 95% CI 0.64–0.89). Multivariable analysis showed a significant reduction in the IHM over time for men and women with COPD. Suffering from COPD only increased the risk of IHM among men (OR 1.07; 95% CI 1.01–1.13). (4) Conclusions: The incidence of UTIs increased over time. Suffering COPD increased the risk of IHM among men, but not among women.
OCT Angiography Findings in Macula-ON and Macula-OFF Rhegmatogenous Retinal Detachment: A Prospective Study
Authors: Barca Francesco , Bacherini Daniela , Dragotto Francesco , Tartaro Ruggero , Lenzetti Chiara , Finocchio Lucia , Virgili Gianni , Caporossi Tomaso , Giansanti Fabrizio , Savastano Alfonso , Rizzo Stanislao
Published: December 2020

Background: The aim of the study was to evaluate pre-operative and post-operative retinal vasculature using optical coherence tomography angiography (OCTA) in patients who underwent rhegmatogenous retinal detachment (RRD) surgery repair. Materials and Methods: A total of 33 eyes were included in this prospective consecutive observational study: 15 affected by macula-ON and 18 by macula-OFF RRD. Superficial (SCP), deep capillary plexus (DCP), and foveal avascular zone (FAZ) area variations were evaluated by OCTA and correlated with visual acuity (VA) during a six-month follow-up. Results: In the macula-ON group, the preoperative vascular density (VD) of the whole SCP (wSCP) on affected eyes was lower than that of the fellow eyes (p < 0.05); this difference disappeared at 6 months after surgery (p = 0.88). The wSCP VD and the parafoveal SCP (pfSCP) VD increased during follow-up (p < 0.05); moreover, the higher the preoperative wSCP and pfSCP VD, the better the baseline VA (p < 0.05). In the macula-OFF group, at the first and sixth months after surgery, the larger the FAZ, the lower the VA (p < 0.05). Conclusions: Macula-ON SCP VD affected preoperative VA, and it was lower than the fellow eye, but recovered over time. In the macula-OFF group, a larger FAZ area was related to a worse VA, as is the case in diabetes and in retinal vein occlusion (RVO).