bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Supratherapeutic tacrolimus concentrations with nirmatrelvir/ritonavir in a lung transplant patient: a case report using Rifampin for reversal
Authors: Xiong Yu , Wang Xiaoxing , Li Shu , Zhang Qian , Guo Lijuan , Chen Wenhui , Zhao Zhixia , Liu Lihong
Published: November 2023

Paxlovid (nirmatrelvir/ritonavir) is an antiviral drug used to treat COVID-19, nirmatrelvir, a SARS-CoV-2 main protease inhibitor, works by inhibiting viral replication in the early stages, and ritonavir is a strong cytochrome P450 (CYP) 3A inhibitor that helps the nirmatrelvir reach and maintain the therapeutic concentrations. Paxlovid has a potential risk of drug interaction by elevating the plasma concentration of other drugs metabolized by CYP3A, like tacrolimus. This report examines the case of a 57-year-old female lung transplant patient self-administered Paxlovid for 5 days without discontinuing tacrolimus. She presented to the hospital with symptoms of headache, dizziness, palpitations, abdominal distension, nausea, vomiting, and diarrhea. The patient presented with tacrolimus toxicity and the blood concentration of tacrolimus was measured at 106 ng/mL. Urgent medical intervention was initiated, and Rifampin was administered to induce enzyme activity and rapidly decrease the concentration of tacrolimus. By adjusting the tacrolimus dosage, the final concentration was brought within the appropriate range. Clinical pharmacists should prioritize medication education for transplant patients to prevent severe drug interactions and minimize the impact on the patient’s overall well-being.
The relaxometry hype cycle
Authors: Stikov Nikola , Karakuzu Agâh
Published: November 2023

Relaxometry is a field with a glorious and controversial history, and no review will ever do it justice. It is full of egos and inventions, patents and lawsuits, high expectations and deep disillusionments. Rather than a paragraph dedicated to each of these, we want to give it an impressionistic overview, painted over with a coat of personal opinions and ruminations about the future of the field. For those unfamiliar with the Gartner hype cycle, here’s a brief recap. The cycle starts with a technology trigger and goes through a phase of unrealistically inflated expectations. Eventually the hype dies down as implementations fail to deliver on their promise, and disillusionment sets in. Technologies that manage to live through the trough reach the slope of enlightenment, when there is a flurry of second and third generation products that make the initial promise feel feasible again. Finally, we reach the slope of productivity, where mainstream adoption takes off, and more incremental progress is made, eventually reaching steady state in terms of the technology’s visibility. The entire interactive timeline can be viewed at https://qmrlab.org/relaxometry/.
Effect of circadian rhythm on NAD and other metabolites in human brain
Authors: Cuenoud Bernard , Huang Zhiwei , Hartweg Mickael , Widmaier Mark , Lim SongI. , Wenz Daniel , Xin Lijing
Published: November 2023

Nicotinamide Adenine Dinucleotide (NAD) plays a central role in the master circadian clock of the brain (the suprachiasmatic nuclei, SCN) as demonstrated in many model organisms. NAD acts as an enzyme co-factor and substrate and its modulation was found to be tightly regulated to the periodicity of the cycles. However, in human brain, the effect of the circadian rhythm (CR) on the metabolism of the SCN and other brain regions is poorly understood. We conducted a magnetic resonance spectroscopy (MRS) study at a high magnetic field, measuring the occipital brain NAD levels and other metabolites in two different morning and afternoon diurnal states in 25 healthy participants. Salivary cortisol levels were determined to confirm that the experiment was done in two chronologically different physiological conditions, and a behavioral test of risk-taking propensity was administered. Overall, we found that the CR did not significantly affect NAD levels in the occipital brain region. The other brain metabolites measured, including lactate, were not significantly affected by the CR either, except for taurine. The CR did impact risk-taking behavior and salivary cortisol level, confirming that the participants were in two circadian different behavioral and physiological states in the morning and in the afternoon. Measurement of the CR effect on NAD and taurine levels in other brain regions might provide stronger effects.
Effects of H2SO4, GA3, and cold stratification on the water content, coat composition, and dormancy release of Tilia miqueliana seeds
Authors: Wu Yu , Huang Wen Hui , Peng Chen Yin , Shen Yong Bao , Visscher Anne M. , Pritchard Hugh W. , Gao Qiu , Sun Xiao Rui , Wang Ming Zhu , Deng Zhiyun
Published: November 2023

Introduction

Tilia miqueliana is an endemic species whose population is declining. The permeability barrier and mechanical constraint of the pericarp (seed coat) are important causes of its seed dormancy. Although there has been considerable research on this subject, questions remain regarding how the permeability barrier and mechanical constraint of the seed coat are eliminated during dormancy release and how water enters the seed. Therefore, protecting the species by improving its germination/dormancy breaking in the laboratory is urgent

Methods

In this study, the changes in the cellular structure, mechanical properties, and components of the Tilia miqueliana seed coat after an H2SO4-gibberellic acid (GA3) treatment were analyzed during dormancy release. Various analyses (e.g., magnetic resonance imaging, scanning electron microscopy, and paraffin section detection) revealed the water gap and water channel.

Results

The H2SO4 treatment eliminated the blockage at the micropyle and hilum of the seeds. Water entered the seeds through the water gap (micropyle) rather than through the hilum or seed coat, after which it dispersed along the radicle, hypocotyl, and cotyledon to the endosperm. During the cold stratification period, the cellular structure was damaged and an increasing number of holes appeared on the inner and outer surfaces of the seed coat. Vickers hardness tests showed that GA3 decreased the seed coat hardness. Additionally, the seed coat lignin and total phenol contents continuously decreased during the cold stratification period. Notably, the Liquid chromatography–mass spectrometry (LC–MS) analysis of the seed coat detected polyethylene glycol (osmoregulator), which may have destabilized the water potential balance inside and outside the seed and increased the water content to levels required for germination, ultimately accelerating seed dormancy release.

Discussion

This sophisticated and multi-level study reveals how H2SO4 and GA3 eliminate the permeability barrier and mechanical constraints of the seed coat during dormancy release of Tilia miqueliana seeds. This will be beneficial to artificially assist the natural regeneration and population expansion of Tilia miqueliana.
Screening of potential donors for anaerobic stress tolerance during germination in rice
Authors: Mwakyusa Lupakisyo , Heredia Maria Cristina , Kilasi Newton Lwiyiso , Madege Richard R. , Herzog Max , Dixit Shalabh
Published: November 2023

The rising cost of transplanting rice has made direct seeding an affordable alternative for rice establishment, particularly in Africa. However, direct seeding, while cost-effective, faces crop establishment challenges due to flooding. Uncontrolled water, driven by erratic rains in low-lying areas or uneven fields, limit germination. Rice possesses the unique ability of anaerobic germination, enabling it to sprout and emerge in oxygen-deprived conditions. Understanding rice’s response to anaerobic stress during germination is crucial for resilience breeding. Africa, although relying on direct seeding, has made limited progress in addressing flooding during germination compared to Asia. Anaerobic stress tolerance ensures successful crop emergence even in oxygen-limited environments and can help suppress weeds, a significant challenge in direct-seeded rice cultivation. This study aims to contribute by screening for potential rice donors exhibiting anaerobic stress tolerance. We screened 200 rice genotypes at Sokoine University of Agriculture (SUA) in Morogoro, Tanzania, primarily focusing on landraces with untapped potential. Using an alpha lattice design, we conducted two anaerobic experiments in September and October 2022, adding 7 cm of standing water immediately after dry seeding for flooded and maintaining a 2 cm water level after germination in the control for duration of 21 days. We identified potential donors based on selection index computed from genomic estimated breeding values (GEBVs) using eight variables: germination at 14 DAS, germination at 21 DAS, seedling height at 14 DAS, seedling height at 21 DAS, shoot dry matter at 21 DAS, root dry matter at 21 DAS, culm diameter at 21 DAS, and root length at 21DAS. Ten genotypes emerged as the most promising, exhibiting at least 70% germination in floodwater at 21 DAS and greater selection indices. These genotypes were like: Afaa Mwanza 1/159, Rojomena 271/10, Kubwa Jinga, Wahiwahi, Magongo ya Wayungu, Mpaka wa Bibi, Mwangaza, Tarabinzona, IB126-Bug 2013A, and Kanamalia with respective percentages of 75, 74, 71, 86, 75, 80, 71, 80, 70, and 73. These findings contribute to global efforts to mitigate the impacts of flooding during germination. These donors, will be potential to enrich the gene pool for anaerobic germination, providing valuable resources for breeding for flooding tolerance.
Fine mapping of qBK1.2, a major QTL governing resistance to bakanae disease in rice
Authors: Kushwaha Amar Kant , Ellur Ranjith Kumar , Maurya Sarvesh Kumar , Krishnan S. Gopala , Bashyal Bishnu Maya , Bhowmick Prolay Kumar , Vinod K. K. , Bollinedi Haritha , Singh Nagendra Kumar , Singh Ashok Kumar
Published: November 2023

Bakanae disease caused by Fusarium fujikuroi is an emerging disease of rice causing losses in all rice-growing regions around the world. A BC2F2 population was developed by backcrossing the recurrent parent Pusa Basmati 1121 (PB1121) with the recombinant inbred line RIL28, which harbors a major quantitative trait locus (QTL) governing resistance to bakanae, qBK1.2. MassARRAY-based single-nucleotide polymorphism (SNP) assays targeting the genomic region of qBK1.2 helped in fine mapping the QTL to a region of 130 kb between the SNP markers rs3164311 and rs3295562 using 24 recombinants. In-silico mining of the fine-mapped region identified 11 putative candidate genes with functions related to defense. The expression analysis identified two significantly differentially expressed genes, that is, LOC_Os01g06750 and LOC_Os01g06870, between the susceptible genotype PB1121 and the resistant genotypes Pusa1342 and R-NIL4. Furthermore, the SNPs identified in LOC_Os01g06750 produced minor substitutions of amino acids with no major effect on the resistance-related functional motifs. However, LOC_Os01g06870 had 21 amino acid substitutions, which led to the creation of the leucine-rich repeat (LRR) domain in the resistant genotype Pusa1342, thereby making it a potential candidate underlying the major bakanae-resistant QTL qBK1.2. The markers used in the fine mapping program are of immense utility in marker-assisted breeding for bakanae resistance in rice.
Issue when expressing a recombinant protein under the control of p35S in Nicotiana tabacum BY-2 cells
Authors: Navarre Catherine , Orval Rik , Peeters Marie , Bailly Nicolas , Chaumont François
Published: November 2023

Several recombinant proteins have been successfully produced in plants. This usually requires Agrobacterium-mediated cell transformation to deliver the T-DNA into the nucleus of plant cells. However, some genetic instability may threaten the integrity of the expression cassette during its delivery via A. tumefaciens, especially when the protein of interest is toxic to the bacteria. In particular, we found that a Tn3 transposon can be transferred from the pAL4404 Ti plasmid of A. tumefaciens LBA4404 into the expression cassette when using the widely adopted 35S promoter, thereby damaging T-DNA and preventing correct expression of the gene of interest in Nicotiana tabacum BY-2 suspension cells.
Multilingualism, multicultural experience, cognition, and creativity
Authors: Fürst Guillaume , Grin François
Published: November 2023

The once widely held notion that bilingualism is related to enhanced cognitive functions has recently been challenged, in particular among young adults, as opposed to children and older adults. This strand of research, however, is essentially focused on executive functions (e.g., attention, inhibition, and shifting). But there is another side to the bilingualism-cognition story. Indeed, growing evidence has shown that bilingualism, and by extension multilingualism, are associated with enhanced creativity. However, this relation is arguably quite complex, for several reasons. First, creativity is a fuzzy notion; it is usually conceptualized as a mix of cognitive, personality and motivational factors. Second, multilingual people generally have a richer multicultural experience than monolingual people. In addition, multicultural experience itself is also positively related to creativity. Hence, there are manifold relations between cognition, creativity, multilingualism, and multicultural experience. In this brief research report, using a latent variables model which replicates some of our recent findings, we show that both multilingualism and multicultural experience are positively associated with creativity, even when controlling for cognitive abilities (divergent thinking and intelligence). We discuss these results in a perspective that considers methodological challenges and factors that are relevant to goal-directed behavior.
A multisensory mindfulness experience: exploring the promotion of sensory awareness as a mindfulness practice
Authors: Finck Carolyn , Avila Alba , Jiménez-Leal William , Botero Juan Pablo , Shambo Daniel , Hernandez Susana , Reinoso-Carvalho Felipe , Andonova Veneta
Published: November 2023

Objectives

In this preliminary and multidisciplinary exploratory study, we assessed whether a mindfulness practice could be enhanced through a multisensory experience design that mimics the “beginner’s mind,” relying on sensory awareness and biofeedback processes as participants interact with the experience.

Methods

We piloted and designed two conditions, being (a) a guided mindfulness practice based on the senses as an anchor to the present moment, using audio instruction only; and (b) an experience of mindfulness practice with successive sensory stimulation (olfactory, audio, and visual stimulation) followed by an interactive experience with biofeedback that provides a visual representation of the person’s heartbeat in real-time. For each of the conditions we assessed anxiety (state and trait), as well as other psychological variables pre- and post-experience. Additionally, we measured the heart rate variability (HRV) at baseline, during each stage of the experience as well as post intervention.

Results

We collected valid data for a total of 68 individuals. Both groups were similar regarding mean age, sex, and occupation and had similar prior experience with mindfulness. There were no significant differences regarding prior state or trait anxiety between the groups. Analysis of the physiological variables showed that for both groups there was an increase in the parasympathetic activity after the multisensory experience, with small differences in the conditions of stimulation. We did not observe significant differences between the pre and post measures for state of test anxiety. The observed parasympathetic activity variations after both experiences compared with pre and post-surveys demonstrate the importance of physiological vs psychological inspection beyond the common human rational experience that is not always resonate with the body’s response and impacts the needed literacy to self-awareness of emotional well-being.

Conclusion

Participants in both conditions could effectively connect with the experience, while achieving a physiological response different from their baseline state. The acceptance of the designed stimuli was very high, although more research is still needed to uncover its full potential. In sum, the design of multisensory experiences using technology to create an interactive connection with the sensory stimulus, is a promising field in mindfulness and especially in practices involving sensory awareness through the monitoring of parasympathetic activity as an inference indicator of the present-moment connection.
The origins and function of musical performance
Authors: Dunbar Robin I. M.
Published: November 2023

Music is widely recognised as a human universal, yet there is no agreed explanation for its function, or why and when it evolved. I summarise experimental evidence that the primary function of musicking lies in social bonding, both at the dyadic and community levels, via the effect that performing any form of music has on the brain’s endorphin system (the principal neurohormonal basis for social bonding in primates). The many other functions associated with music-making (mate choice, pleasure, coalition signalling, etc) are all better understood as derivative of this, either as secondary selection pressures or as windows of evolutionary opportunity (exaptations). If music’s function is primarily as an adjunct of the social bonding mechanism (a feature it shares with laughter, feasting, storytelling and the rituals of religion), then reverse engineering the problem suggests that the capacity for music-making most likely evolved with the appearance of archaic humans. This agrees well with anatomical evidence for the capacity to sing.
Exploring the inherent mechanism of residents’ participation behavior in neighborhood regeneration projects: an empirical study using an extended IMB model in China
Authors: Fu Xinyue , Zhuang Taozhi , Huang Ruopeng , Dong Yaxian
Published: November 2023

Introduction

Resident participation has gained increasing prominence and significance in the pursuit of sustainable neighborhoods regeneration. However, the current state of resident participation practices remains beset by several challenges, which present formidable impediments to the initiation and execution of neighborhood regeneration initiatives. This study aims to investigate the underlying mechanisms of residents’ participation behavior to enhance resident participation in neighborhood regeneration projects.

Methods

The present study employs the extended Information-Motivation-Behavioral (IMB) model to examine the determinants and mechanisms influencing residents’ willingness and participation in neighborhood regeneration projects, with a specific focus on the Chinese context. Drawing upon data from 477 meticulously validated questionnaires administered to residents, the study applies structural equation modeling (SEM) to unravel the intrinsic dynamics of residents’ participation behavior.

Results

The empirical findings of this research reveal that information, motivation, and the perceived local government support all exert a significant impact on residents’ participation willingness. Notably, motivation emerges as the most influential factor.

Discussion

This study uncovers a direct influence of local government organizations on both residents’ willingness and their actual participation, suggesting that government organizations can spearhead innovative strategies to bolster residents’ willingness and furnish avenues for translating willingness into tangible participation. The outcomes of this study furnish an indispensable theoretical framework and offer policy recommendations that hold paramount importance for the deployment of novel interventions geared toward stimulating active involvement of residents in neighborhood regeneration.
An experimental study of information transparency and social preferences on donation behaviors: the self-signaling model
Authors: She Minnie H. C. , Sanfey Alan G.
Published: November 2023

Certain segments of the population reply on charitable or other non-governmental organizations as their main source of support, with these organizations largely funded by those in society who can afford to give. The present study investigated to what extent information transparency influences donation decisions, and whether specific preferences for charities influences information seeking behavior. We recruited 114 participants via Prolific and employed a binary online Dictator Game to address these two study objectives. The results showed that participants’ actual donation behavior was not influenced by their charity preference or the level of information transparency. However, they were more prone to seek out additional information when deciding about the most preferred category of charity. These results raise important questions as to whether the perceived anonymity of online choices may differ from choices carried out in person.
Empowerment of the older adults in the context of Chinese culture: an evolutionary concept analysis
Authors: Zhang Shibo , Li Junfeng , Zou Jingjie , Ai Yating , Qin Siqi , Xiao Xixi , Hu Hui , Wang Yuncui
Published: November 2023

Background

With the increasing focus on addressing the challenges of aging, researchers have begun to recognize the potential impact of empowering older individuals in addressing retirement issues. However, within different cultural contexts, there still needs to be a more precise analysis regarding the definition of empowering older individuals.

Objective

To define and analyze the concept of empowering older individuals within the cultural context of China.

Method

Using Rodgers’ concept analysis approach, a search was conducted in five databases (PubMed, Web of Science, China National Knowledge Infrastructure, Wan fang Data, and VIP Chinese Journal Platform) for studies on empowering older individuals from the time the databases were established until February 2023. The main disciplines involved in the search included nursing, medicine, and public health.

Results

Out of the 7,028 studies, 50 articles met the inclusion criteria. The identified attributes are as follows: support system, belief change, and behavioral autonomy. The antecedents were grouped into four categories: physical obstacles, psychological concerns, personal needs and external challenges. The consequences were determined to be improved quality of life, reduced burden of old-age care, gain respect, and self-actualization.

Conclusion

Empowering older individuals is a dynamic and evolving concept that involves aligning personal aspirations with appropriate external resources and expressing a certain degree of belief and behavioral change. This study deepens our understanding of empowering older individuals through comprehensive concept analysis, and the identified attributes, antecedents, and consequences of empowering older individuals can be utilized in practice, education, and research.
Integrated behavioral health services in pediatric primary care and emergency department utilization for suicide risk
Authors: Wellen Brianna C. M. , Wright Naomi M. , Bickford Mira A. , Bakken Eliza Hayes , Riley Andrew R.
Published: November 2023

Introduction

Universal screening for suicide risk in primary care settings is a promising avenue for preventing self-harm and improving health outcomes. Triaging youth to an appropriate level of care, including diverting lower-risk patients from the emergency department (ED) is a meaningful goal. Previous research indicates integrated behavioral health (IBH) may prevent unnecessary admission to the ED on the day of suicide risk screening. We hypothesized that youth who received an IBH consultation the same day as suicide risk screening would be less likely to be admitted to the ED, but more likely to contact IBH services and utilize primary care in the following month.

Methods

We conducted a retrospective chart review of 3,649 youth aged 10-18 years who were screened with the Ask Suicide-Screening Questions (ASQ) in two pediatric primary care practices. We collected demographic data, ASQ and Patient Health Questionnaire-9 (PHQ-9) scores, as well as patient contacts with IBH, the ED, and medical primary care the day of screening and the following 31 days. We conducted a series of logistic regressions and chi-square analyses to determine whether contact with IBH on the same day as positive suicide risk screenings predicted same-day admission to the ED, IBH contact, and medical primary care utilization.

Results

Among the 7,982 ASQ scores, 1,380 (18%) were non-acute and 87 ASQs (1%) screened acutely positive. Over 90% of positive screens were diverted from the ED regardless of IBH contact. None of the patients died from suicide. Same-day IBH was associated with higher likelihood of general ED visits for all positive screens (acute and non-acute together). None of the positive screens that received an IBH consultation on the same day as screening were admitted to the ED in the subsequent month. Contact with IBH the same day as screening positively predicted utilization of IBH and medical primary care services in the subsequent month, especially for youth with minority race and ethnicity identities.

Discussion

In the context of clinics with IBH and systematic risk assessment processes, most youth who screen positive for suicide risk are diverted from the ED. However, contrary to our hypothesis, our study showed that youth who received same-day IBH consultations were more likely to be admitted to the ED compared to peers who did not receive IBH consultations. These findings suggest that systematic suicide screening combined with IBH consultations in pediatric primary care can effectively identify risk levels and triage patients to appropriate care.
The role of pharmacogenetics in the treatment of major depressive disorder: a critical review
Authors: Barlati Stefano , Minelli Alessandra , Nibbio Gabriele , Bertoni Lorenzo , Necchini Nicola , Paolini Stefano , Muscarella Alessia , Ubertino Ughetta Bosco , Calzavara-Pinton Irene , Vita Antonio , Gennarelli Massimo
Published: November 2023

Pharmacological therapy represents one of the essential approaches to treatment of Major Depressive Disorder (MDD). However, currently available antidepressant medications show high rates of first-level treatment non-response, and several attempts are often required to find an effective molecule for a specific patient in clinical practice. In this context, pharmacogenetic analyses could represent a valuable tool to identify appropriate pharmacological treatment quickly and more effectively. However, the usefulness and the practical effectiveness of pharmacogenetic testing currently remains an object of scientific debate. The present narrative and critical review focuses on exploring the available evidence supporting the usefulness of pharmacogenetic testing for the treatment of MDD in clinical practice, highlighting both the points of strength and the limitations of the available studies and of currently used tests. Future research directions and suggestions to improve the quality of available evidence, as well as consideration on the potential use of pharmacogenetic tests in everyday clinical practice are also presented.
Community of Practice of Promotoras de Salud to address health inequities during and beyond the COVID-19 pandemic
Authors: Rodriguez Espinosa Patricia , Martinez Mulet Yessica , Chen Wei-ting , Kirk Cary , Tran Cindy , Gonzalez Mike , Rosas Lisa G. ,   Promotoras Con Stanford En Acción
Published: November 2023

Using principles of Community-Based Participatory Research, we describe a community of practice for community health workers and promotoras (CHW/Ps) to address COVID-19 inequities in the Latinx community. We offer a concrete example of how programs can engage CHW/Ps as full partners in the research process, and how programs can support CHW/Ps’ capacity and workforce development during implementation. We conducted four focus groups with CHW/Ps (n = 31) to understand needs and invited 15 participants to the community of practice to work on issues identified by the group. We examined impact according to number of community members reached, types of outreach activities, surveys, and online views of educational materials. Process evaluation involved two focus groups with seven organizations and a Ripple Effects Mapping session with the CHW/Ps. Our community of practice has built CHW/Ps’ capacity via 31 workshop and co-created culturally and linguistically relevant COVID-19 materials that have reached over 40,000 community members and over 3 million people online. The community of practice proved effective in supporting CHW/Ps to address COVID-19 inequities in the Latinx community. Our evaluations demonstrated benefits for community-academic partnerships, for CHW/Ps, and for the community. This model represents an innovative workforce training model to address health inequities and can be applied to other health topics.
Disability risk prediction model based on machine learning among Chinese healthy older adults: results from the China Health and Retirement Longitudinal Study
Authors: Han Yuchen , Wang Shaobing
Published: November 2023

Background

Predicting disability risk in healthy older adults in China is essential for timely preventive interventions, improving their quality of life, and providing scientific evidence for disability prevention. Therefore, developing a machine learning model capable of evaluating disability risk based on longitudinal research data is crucial.

Methods

We conducted a prospective cohort study of 2,175 older adults enrolled in the China Health and Retirement Longitudinal Study (CHARLS) between 2015 and 2018 to develop and validate this prediction model. Several machine learning algorithms (logistic regression, k-nearest neighbors, naive Bayes, multilayer perceptron, random forest, and XGBoost) were used to assess the 3-year risk of developing disability. The optimal cutoff points and adjustment parameters are explored in the training set, the prediction accuracy of the models is compared in the testing set, and the best-performing models are further interpreted.

Results

During a 3-year follow-up period, a total of 505 (23.22%) healthy older adult individuals developed disabilities. Among the 43 features examined, the LASSO regression identified 11 features as significant for model establishment. When comparing six different machine learning models on the testing set, the XGBoost model demonstrated the best performance across various evaluation metrics, including the highest area under the ROC curve (0.803), accuracy (0.757), sensitivity (0.790), and F1 score (0.789), while its specificity was 0.712. The decision curve analysis (DCA) indicated showed that XGBoost had the highest net benefit in most of the threshold ranges. Based on the importance of features determined by SHAP (model interpretation method), the top five important features were identified as right-hand grip strength, depressive symptoms, marital status, respiratory function, and age. Moreover, the SHAP summary plot was used to illustrate the positive or negative effects attributed to the features influenced by XGBoost. The SHAP dependence plot explained how individual features affected the output of the predictive model.

Conclusion

Machine learning-based prediction models can accurately evaluate the likelihood of disability in healthy older adults over a period of 3 years. A combination of XGBoost and SHAP can provide clear explanations for personalized risk prediction and offer a more intuitive understanding of the effect of key features in the model.
Rothmund-Thomson syndrome, a disorder far from solved
Authors: Martins Davi Jardim , Di Lazzaro Filho Ricardo , Bertola Debora Romeo , Hoch Nícolas Carlos
Published: November 2023

Rothmund-Thomson syndrome (RTS) is a rare autosomal recessive disorder characterized by a range of clinical symptoms, including poikiloderma, juvenile cataracts, short stature, sparse hair, eyebrows/eyelashes, nail dysplasia, and skeletal abnormalities. While classically associated with mutations in the RECQL4 gene, which encodes a DNA helicase involved in DNA replication and repair, three additional genes have been recently identified in RTS: ANAPC1, encoding a subunit of the APC/C complex; DNA2, which encodes a nuclease/helicase involved in DNA repair; and CRIPT, encoding a poorly characterized protein implicated in excitatory synapse formation and splicing. Here, we review the clinical spectrum of RTS patients, analyze the genetic basis of the disease, and discuss molecular functions of the affected genes, drawing some novel genotype-phenotype correlations and proposing avenues for future studies into this enigmatic disorder.
Understanding the need for digital twins’ data in patient advocacy and forecasting oncology
Authors: Chang Hung-Ching , Gitau Antony M. , Kothapalli Siri , Welch Danny R. , Sardiu Mihaela E. , McCoy Matthew D.
Published: November 2023

Digital twins are made of a real-world component where data is measured and a virtual component where those measurements are used to parameterize computational models. There is growing interest in applying digital twins-based approaches to optimize personalized treatment plans and improve health outcomes. The integration of artificial intelligence is critical in this process, as it enables the development of sophisticated disease models that can accurately predict patient response to therapeutic interventions. There is a unique and equally important application of AI to the real-world component of a digital twin when it is applied to medical interventions. The patient can only be treated once, and therefore, we must turn to the experience and outcomes of previously treated patients for validation and optimization of the computational predictions. The physical component of a digital twins instead must utilize a compilation of available data from previously treated cancer patients whose characteristics (genetics, tumor type, lifestyle, etc.) closely parallel those of a newly diagnosed cancer patient for the purpose of predicting outcomes, stratifying treatment options, predicting responses to treatment and/or adverse events. These tasks include the development of robust data collection methods, ensuring data availability, creating precise and dependable models, and establishing ethical guidelines for the use and sharing of data. To successfully implement digital twin technology in clinical care, it is crucial to gather data that accurately reflects the variety of diseases and the diversity of the population.
QREChem: quantum resource estimation software for chemistry applications
Authors: Otten Matthew , Kang Byeol , Fedorov Dmitry , Lee Joo-Hyoung , Benali Anouar , Habib Salman , Gray Stephen K. , Alexeev Yuri
Published: November 2023

As quantum hardware continues to improve, more and more application scientists have entered the field of quantum computing. However, even with the rapid improvements in the last few years, quantum devices, especially for quantum chemistry applications, still struggle to perform calculations that classical computers could not calculate. In lieu of being able to perform specific calculations, it is important have a systematic way of estimating the resources necessary to tackle specific problems. Standard arguments about computational complexity provide hope that quantum computers will be useful for problems in quantum chemistry but obscure the true impact of many algorithmic overheads. These overheads will ultimately determine the precise point when quantum computers will perform better than classical computers. We have developed QREChem to provide logical resource estimates for ground state energy estimation in quantum chemistry through a Trotter-based quantum phase estimation approach. QREChem provides resource estimates which include the specific overheads inherent to problems in quantum chemistry by including heuristic estimates of the number of Trotter steps and number of necessary ancilla, allowing for more accurate estimates of the total number of gates. We utilize QREChem to provide logical resource estimates for a variety of small molecules in various basis sets, obtaining estimates in the range of 107–1015 for total number of T gates. We also determine estimates for the FeMoco molecule and compare all estimates to other resource estimation tools. Finally, we compare the total resources, including hardware and error correction overheads, demonstrating the need for fast error correction cycle times.
The Human Microbiome and Its Role in Keeping Us Healthy
Authors: Saadaoui Marwa , Al Khodor Souhaila
Published: November 2023

Humans are complex ecosystems made up of human cells and small organisms called microbes. The diverse group of microbes living in and on the body is called the microbiome. By looking at the genes of these organisms, the Human Microbiome Project showed that each part of the body has its own set of microbes. For the body to be in good health, the microbiome must be in balance. The microbiome keeps us healthy in several ways. For example, it helps digest and absorb nutrients, train the immune system, and protect us from harmful microbes and chronic infections. Changes to the normal composition of the microbiome, known as dysbiosis, can lead to certain diseases. In this article, we will explain the role of the microbiome in maintaining human health. We will also explain the influence of factors like genes, age, diet, antibiotic use, lifestyle, and others on the composition of the microbiome and some of the diseases that can result.
Drivers affecting the relation between biodistricts and school meals initiatives: evidence from the Cilento biodistrict
Authors: Kraljevic Beatrice , Zanasi Cesare
Published: November 2023

Biodistricts (also known as “organic districts” or “eco-regions”) are receiving increasing attention by scholars and public institutions. This interest is based largely on the biodistricts’ focus on linking organic agriculture with the socio-economic, cultural and ethical aspects of the areas in which they are located. Furthermore, these realities are in line with recent EU initiatives aimed at promoting sustainable practices, such as Green Public Procurement and others included in the Farm to Fork strategy. The rising awareness of sustainability, food safety and security within schools has made Public School Food Procurement (PSFP) one of the potential drivers supporting the biodistricts’ development. Despite its relevance, research on this topic is relatively scarce. The present study seeks to assess the drivers influencing the integration between biodistricts and organic PSFP. A theoretical and an analytical framework tool were developed to this end and applied to the Cilento Biodistrict, where organic PSFP is currently active. The research highlighted different drivers positively affecting the access of the Cilento Biodistrict to organic PSFP, mainly related to its collaborative nature and the interest in adopting more sustainable practices. However, characteristics of local organic production such as high prices and insufficient volumes negatively impact the relationship with organic public school canteens. Considering the relatively homogeneous characteristics of these realities, the study provides a reference framework for further research and policies supporting the relationship between organic school meals initiatives and biodistricts.
In silico approaches in carcinogenicity hazard assessment: case study of pregabalin, a nongenotoxic mouse carcinogen
Authors: Keller Douglas A. , Bassan Arianna , Amberg Alexander , Burns Naas Leigh Ann , Chambers Jon , Cross Kevin , Hall Frances , Jahnke Gloria D. , Luniwal Amarjit , Manganelli Serena , Mestres Jordi , Mihalchik-Burhans Amy L. , Woolley David , Tice Raymond R.
Published: November 2023

In silico toxicology protocols are meant to support computationally-based assessments using principles that ensure that results can be generated, recorded, communicated, archived, and then evaluated in a uniform, consistent, and reproducible manner. We investigated the availability of in silico models to predict the carcinogenic potential of pregabalin using the ten key characteristics of carcinogens as a framework for organizing mechanistic studies. Pregabalin is a single-species carcinogen producing only one type of tumor, hemangiosarcomas in mice via a nongenotoxic mechanism. The overall goal of this exercise is to test the ability of in silico models to predict nongenotoxic carcinogenicity with pregabalin as a case study. The established mode of action (MOA) of pregabalin is triggered by tissue hypoxia, leading to oxidative stress (KC5), chronic inflammation (KC6), and increased cell proliferation (KC10) of endothelial cells. Of these KCs, in silico models are available only for selected endpoints in KC5, limiting the usefulness of computational tools in prediction of pregabalin carcinogenicity. KC1 (electrophilicity), KC2 (genotoxicity), and KC8 (receptor-mediated effects), for which predictive in silico models exist, do not play a role in this mode of action. Confidence in the overall assessments is considered to be medium to high for KCs 1, 2, 5, 6, 7 (immune system effects), 8, and 10 (cell proliferation), largely due to the high-quality experimental data. In order to move away from dependence on animal data, development of reliable in silico models for prediction of oxidative stress, chronic inflammation, immunosuppression, and cell proliferation will be critical for the ability to predict nongenotoxic compound carcinogenicity.
Role of mucin 2 gene for growth in Anas platyrhynchos: a novel report
Authors: Kumar Murmu Anuj , Pal Aruna , Debnath Manti , Chakraborty Argha , Pal Subhamoy , Banerjee Samiddha , Pal Abantika , Ghosh Nilotpal , Karmakar Utpal , Samanta Rajarshi
Published: November 2023

Introduction

The mucin gene is expressed in the mucous membrane of the inner layer of the internal organs. Intestinalmucin 2 (MUC2), amajor gel-formingmucin, represents a primary barrier component of mucus layers.

Materials and methods

This is the first report on the role of mucin genes in growth traits in animals. In this study, we randomly studied Bengal ducks (Anas platyrhynchos) reared from day old to 10 weeks of age under an organized farm and studied the growth parameters as well as body weight and average daily body weight gain.

Result and discussion

We characterized the mucin gene for Bengal ducks and observed glycosylation and EGF1 (EGF-like domain signature) as important domains for growth traits in ducks. We observed a better expression profile for the mucin gene in high-growing ducks in comparison to that of low-growing ducks with real-time PCR. Hence, the mucin gene may be employed as a marker for growth traits.
Genotypic and antimicrobial susceptibility of Streptococcus agalactiae causing bovine mastitis in the central region of Thailand
Authors: Wataradee Sirirat , Samngamnim Sukuma , Boonserm Thanasak , Ajariyakhajorn Kittisak
Published: November 2023

Introduction

Streptococcus agalactiae is a highly contagious pathogen that causes bovine mastitis, leading to significant economic losses. This study aimed to (1) identify and characterize S. agalactiae strains responsible for bovine mastitis by examining their phenotypic and genotypic characteristics in Thai dairy-intensive farming areas and (2) determine their susceptibility profiles to antimicrobial agents.

Material and methods

In total, 100 S. agalactiae isolates obtained from clinical and subclinical mastitis cases from 13 dairy herds located in the central region of Thailand were examined. To confirm the identity of the bacterial pathogens, conventional microbiological procedures recommended by the National Mastitis Council (NMC) and the VITEK® 2 system were employed.

Results

All 100 isolates were successfully identified as S. agalactiae using the NMC procedure, whereas 94 isolates were identified as S. agalactiae using the VITEK® 2 system. Finally, the S. agalactiae-specific gene dlt S was identified in all the examined isolates using polymerase chain reaction. Capsular polysaccharide (CPS) typing revealed that all strains belonged to CPS type Ia. Multilocus sequence typing identified 33 selected isolates as sequence type 103. Random amplified polymorphic DNA (RAPD) typing yielded 43 RAPD types, with 6 RAPD clusters identified. These results demonstrated a high level of genetic diversity among S. agalactiae within the studied herds. RAPD analysis suggested that specific S. agalactiae strains could persist in dairy farms for 2–12 months. Furthermore, antimicrobial susceptibility testing was performed using the broth microdilution method. Most strains demonstrated susceptibility to ampicillin, penicillin, penicillin/novobiocin, cephalothin, oxacillin, ceftiofur, and erythromycin.

Discussion

This study revealed the phenotypic and genotypic characteristics of S. agalactiae isolates responsible for bovine mastitis in the central region of Thailand. The rapid identification of S. agalactiae and application of molecular typing methods can provide valuable epidemiological information regarding S. agalactiae causing mastitis in dairy farms. The antimicrobial susceptibility of S. agalactiae indicates that antimicrobial treatment for control and eradication could be a successful protocol. Our findings revealed that a single clonal strain of S. agalactiae affected the 13 studied farms. Further research is needed to explore the feasibility of vaccine development and application.
Goat hair as a bioindicator of environmental contaminants and adrenal activation during vertical transhumance
Authors: Agradi Stella , Munga Albana , Barbato Olimpia , Palme Rupert , Tarhan Duygu , Bilgiç Bengü , Dokuzeylül Banu , Ercan Alev Meltem , Or Mehmet Erman , Brecchia Gabriele , Curone Giulio , Draghi Susanna , Vigo Daniele , Marongiu Maria Laura , González-Cabrera Marta , Menchetti Laura
Published: November 2023

Autochthonous breeds of livestock are considered a pivotal genetic resource for agriculture, rural development, and food and nutrition security. In the Italian Alps, local livestock breeds are maintained using the traditional alpine farming system based on vertical transhumance, with the use of alpine pastures from late spring to autumn and indoor housing with a hay-based diet for the remaining part of the year. Because of their tight link with the territory of origin, local breeds could be used to biomonitor environmental contaminations. Moreover, animal welfare should also be monitored during transhumance in animals, which are exposed to a sudden farming system change and different types of stressors. For these reasons, this investigation hypothesized that the content of trace elements, heavy metals, and cortisol in the hair of goats changes during vertical transhumance, possibly reflecting different dietary contents and activity of the hypothalamic–pituitary–adrenal (HPA) axis. This study aimed to assess the response of an Italian local goat breed to the change from indoor housing to alpine pasture in summer in terms of hair concentrations of (i) trace elements and heavy metals and (ii) cortisol. The regrown hair of Frisa goats was monthly collected for 2 consecutive years (n = 10 for heavy metals and trace elements and n = 6 for cortisol in 2021, n = 17 for both analyses in 2022), once before vertical transhumance and twice after that event. Hair was then analyzed for trace elements, heavy metals, and cortisol by inductively coupled plasma-optical emission spectrophotometer (ICP-OES) and enzyme immunoassay (EIA), respectively. Data were analyzed by multilevel models. The results showed an increase in As content during alpine pasture (p < 0.01), probably reflecting the soil and water As contents of the grazing area, while Mg, Zn, and Al (p < 0.01) followed the opposite trend, decreasing in the second month after vertical transhumance. Hair cortisol concentrations increased during 2 months of alpine pasture (p < 0.001), indicating an increase in the activation of the HPA axis, in agreement with previous studies. Future investigations can consider a longer study period and the development of ad hoc animal welfare indicators.