bluuebunny/crossref_metadata_2025 huggingface dataset:
Correction: Zhang et al. Clinical Management of Low Anterior Resection Syndrome: Review of the Current Diagnosis and Treatment. Cancers 2023, 15, 5011
Authors: Zhang Ruijia , Luo Wenqin , Qiu Yulin , Chen Fan , Luo Dakui , Yang Yufei , He Weijing , Li Qingguo , Li Xinxiang
Published: January 2024

In the published publication [...]
CRISPRing KRAS: A Winding Road with a Bright Future in Basic and Translational Cancer Research
Authors: Gong Xian , Du Jianting , Peng Ren-Wang , Chen Chun , Yang Zhang
Published: January 2024

Once considered “undruggable” due to the strong affinity of RAS proteins for GTP and the structural lack of a hydrophobic “pocket” for drug binding, the development of proprietary therapies for KRAS-mutant tumors has long been a challenging area of research. CRISPR technology, the most successful gene-editing tool to date, is increasingly being utilized in cancer research. Here, we provide a comprehensive review of the application of the CRISPR system in basic and translational research in KRAS-mutant cancer, summarizing recent advances in the mechanistic understanding of KRAS biology and the underlying principles of drug resistance, anti-tumor immunity, epigenetic regulatory networks, and synthetic lethality co-opted by mutant KRAS.
Optimal Treatment Strategy for Oligo-Recurrence Lung Cancer Patients with Driver Mutations
Authors: Tachibana Taimei , Matsuura Yosuke , Ninomiya Hironori , Ichinose Junji , Nakao Masayuki , Okumura Sakae , Nishio Makoto , Ikeda Norihiko , Mun Mingyon
Published: January 2024

Background: The efficacy of local therapies for lung cancer patients with postoperative oligo-recurrence has been reported. However, whether local therapies should be chosen over molecular targeted therapies for oligo-recurrence patients with driver mutations remains controversial. Therefore, we aimed to investigate the optimal initial treatment strategy for oligo-recurrence in lung cancer patients with driver mutations. Methods: Among 2152 patients with lung adenocarcinoma who underwent surgical resection at our institute between 2008 and 2020, 66 patients with driver mutations who experienced cancer oligo-recurrence after surgery and were treated with local or molecularly targeted therapy as an initial therapy after recurrence were evaluated. Oligo-recurrence was characterized by the presence of 1 to 3 recurrent lesions. These patients were investigated, focusing on their post-recurrence therapies and prognoses. Results: The median follow-up period was 71 months. Local and molecular targeted therapies were administered to 41 and 25 patients, respectively. The number of recurrence lesions tended to be lower in the initial local therapy group than in the molecular targeted therapy group. In the initial local therapy group, 23 patients (56%) subsequently received molecular targeted therapies. The time from recurrence to the initiation of molecular targeted therapy was significantly longer in the local therapy group than in the molecular targeted therapy group (p < 0.001). There was no significant difference in post-recurrence overall survival (hazard ratio, 1.429; 95% confidence interval, 0.701–2.912; log-rank, p = 0.324) and post-recurrence progression-free survival (hazard ratio, 0.799; 95% confidence interval, 0.459–1.390; log-rank, p = 0.426) in the initial local ablative therapy group compared with the initial molecular targeted therapy group. Conclusions: Local therapies as a first-line treatment did not show statistically significant differences in post-recurrence survival or progression-free survival compared with molecular targeted therapies. However, local therapies as an initial treatment should be considered preferably, as they can cure the recurrence and can delay the start of administration of molecular targeted therapies.
Postoperative Tobacco Cessation Improves Quality of Life, Lung Function and Long-Term Survival in Non-Small-Cell Lung Cancer Patients
Authors: Doerr Fabian , Leschczyk Tobias , Grapatsas Konstantinos , Menghesha Hruy , Baldes Natalie , Schlachtenberger Georg , Heldwein Matthias B. , Michel Maximilian , Quaas Alexander , Hagmeyer Lars , Höpker Katja , Wahlers Thorsten , Darwiche Kaid , Taube Christian , Schuler Martin , Hekmat Khosro , Bölükbas Servet
Published: January 2024

Objectives: About 90% of all non-small cell lung cancer (NSCLC) cases are associated with inhalative tabacco smoking. Half of patients continue smoking during lung cancer therapy. We examined the effects of postoperative smoking cessation on lung function, quality of life (QOL) and long-term survival. Materials and Methods: In total, 641 patients, who underwent lobectomy between 2012 and 2019, were identified from our single institutional data base. Postoperatively, patients that actively smoked at the time of operation were offered a structured ‘smoking cessation’ program. For this retrospective analysis, two patient groups (total n = 90) were selected by pair matching. Group A (n = 60) had no postoperative tobacco smoking. Group B (n = 30) involved postoperative continued smoking. Lung function (FEV1, DLCO) and QOL (‘SF-36′ questionnaire) were measured 12 months postoperatively. We compared long-term outcomes using Kaplan–Meier curves. Results: The mean age in group A was 62.6 ± 12.5 years and that in group B was 64.3 ± 9.7 years (p = 0.82); 64% and 62%, respectively, were male (p = 0.46). Preoperative smoking habits were similar (‘pack years’: group A, 47 ± 31; group B, 49 ± 27; p = 0.87). All relevant baseline characteristics we collected were similar (p > 0.05). One year after lobectomy, FEV1 was reduced by 15% in both groups (p = 0.98). Smoking cessation was significantly associated with improved DLCO (group A: 11 ± 16%; group B: −5 ± 14%; p <0.001) and QOL (vitality (VT): +10 vs. −10, p = 0.017; physical role function (RP): +8 vs. −17, p = 0.012; general health perceptions (GH): +12 vs. −5, p = 0.024). Patients who stopped smoking postoperatively had a significantly superior overall survival (median survival: 89.8 ± 6.8 [95% CI: 76.6–103.1] months vs. 73.9 ± 3.6 [95% CI: 66.9–80.9] months, p = 0.034; 3-year OS rate: 96.2% vs. 81.0%, p = 0.02; 5-year OS rate: 80.0% vs. 64.0%, p = 0.016). The hazard ratio (HR) was 2.31 [95% CI: 1.04–5.13] for postoperative smoking versus tobacco cessation. Conclusion: Postoperative smoking cessation is associated with improved quality of life and lung function testing. Notably, a significant increase in long-term survival rates among non-smoking NSCLC patients was observed. These findings could serve as motivation for patients to successfully complete a non-smoking program.
Epidermolysis-Bullosa-Associated Squamous Cell Carcinomas Support an Immunosuppressive Tumor Microenvironment: Prospects for Immunotherapy
Authors: Rafei-Shamsabadi David , Scholten Lena , Lu Sisi , Castiglia Daniele , Zambruno Giovanna , Volz Andreas , Arnold Andreas , Saleva Mina , Martin Ludovic , Technau-Hafsi Kristin , Meiss Frank , von Bubnoff Dagmar , Has Cristina
Published: January 2024

Cutaneous squamous cell carcinomas (SCCs) are a major complication of some subtypes of epidermolysis bullosa (EB), with high morbidity and mortality rates and unmet therapeutic needs. The high rate of endogenous mutations and the fibrotic stroma are considered to contribute to the pathogenesis. Patients with dystrophic EB (DEB) and Kindler EB (KEB) have the highest propensity for developing SCCs. Another patient group that develops high-risk SCCs is immunosuppressed (IS) patients, especially after organ transplantation. Herein, we interrogate whether immune checkpoint proteins and immunosuppressive enzymes are dysregulated in EB-associated SCCs as an immune resistance mechanism and compare the expression patterns with those in SCCs from IS patients, who frequently develop high-risk tumors and sporadic SCCs, and immunocompetent (IC) individuals. The expression of indoleamine 2,3-dioxygenase (IDO), programmed cell death protein-1 (PD-1), programmed cell death ligand-1 (PD-L1), T cell immunoglobulin and mucin-domain-containing protein-3 (TIM-3), lymphocyte activation gene-3 (LAG-3), and inflammatory infiltrates (CD4, CD8, and CD68) was assessed via immunohistochemistry and semi-quantitative analysis in 30 DEB-SCCs, 22 KEB-SCCs, 106 IS-SCCs, and 100 sporadic IC-SCCs. DEB-SCCs expressed significantly higher levels of IDO and PD-L1 in tumor cells and PD-1 in the tumor microenvironment (TME) compared with SCCs from IC and IS individuals. The number of CD4-positive T cells per mm2 was significantly lower in DEB-SCCs compared with IC-SCCs. KEB-SCCs showed the lowest expression of the exhaustion markers TIM-3 and LAG-3 compared with all other groups. These findings identify IDO, PD-1, and PD-L1 to be increased in EB-SCCs and candidate targets for combinatory treatments, especially in DEB-SCCs.
Catalytic Conversion of Cyclopentanone into Dimethyl Adipate over Solid Basic Catalysts with Dimethyl Carbonate
Authors: Martínez-Salazar Irene , Orozco-Saumell Ana , López Granados Manuel , Mariscal Rafael
Published: January 2024

The synthesis of dimethyl adipate (DAP), a stable configuration of adipic acid, from biomass-derived cyclopentanone (CPO) and dimethyl carbonate (DMC) constitutes an attractive greener route than petroleum-based industrial processes. Solid basic catalysts such as MgO, Mg5(CO3)4(OH)2·4H2O, KOCH3 and Ca(OCH3)2 have been used achieving a DAP yield up to 30% at 533 K. In addition to the type of catalyst, other operating conditions such as the substrate, reaction time, temperature and CPO concentration have been studied. The methylation of DAP and CPO and the self-aldol condensation of CPO to form dimers and oligomers are reactions that occur in parallel with the production of DAP. It has been established that the main challenge is the self-aldol condensation of CPO. It has been identified that at short reaction times, to prevent methylation, and at dilute concentrations, to avoid CPO self-condensation, the DAP formation rate is much higher than these other competitive reactions. Finally, it should be noted that a DAP productivity up to 3.45 g·gcat−1·h−1 has been achieved under mild conditions.
Organocatalysts for the Synthesis of Cyclic Carbonates under the Conditions of Ambient Temperature and Atmospheric CO2 Pressure
Authors: Seong Yeongju , Lee Sanghun , Cho Seungyeon , Kim Yoseph , Kim Youngjo
Published: January 2024

2–(1H–1,2,4–Triazol–3–yl)phenol (CAT–1) was used as an organocatalyst for the coupling reaction of CO2 and epoxides at an ambient temperature and atmospheric CO2 pressure (1 bar). This compound has a structure in which a hydrogen bond donor, a hydrogen bond acceptor, and another hydrogen bond donor are adjacent in sequence in a molecule. The binary catalytic system of CAT–1/nBu4NI showed TON = 19.2 and TOF = 1.60 h−1 under 1 bar CO2 at room temperature within 12 h using 2–butyloxirane. Surprisingly, the activity of CAT–1, in which phenol and 1H–1,2,4–triazole are chemically linked, showed a much greater synergistic effect than when simply mixing the same amount of phenol and 1H–1,2,4–triazole under the same reaction conditions. In addition, our system showed a broad terminal and internal epoxide substrate scope.
Functional Classification and Interaction Selectivity Landscape of the Human SH3 Domain Superfamily
Authors: Kazemein Jasemi Neda S. , Mehrabipour Mehrnaz , Magdalena Estirado Eva , Brunsveld Luc , Dvorsky Radovan , Ahmadian Mohammad R.
Published: January 2024

SRC homology 3 (SH3) domains are critical interaction modules that orchestrate the assembly of protein complexes involved in diverse biological processes. They facilitate transient protein–protein interactions by selectively interacting with proline-rich motifs (PRMs). A database search revealed 298 SH3 domains in 221 human proteins. Multiple sequence alignment of human SH3 domains is useful for phylogenetic analysis and determination of their selectivity towards PRM-containing peptides (PRPs). However, a more precise functional classification of SH3 domains is achieved by constructing a phylogenetic tree only from PRM-binding residues and using existing SH3 domain–PRP structures and biochemical data to determine the specificity within each of the 10 families for particular PRPs. In addition, the C-terminal proline-rich domain of the RAS activator SOS1 covers 13 of the 14 recognized proline-rich consensus sequence motifs, encompassing differential PRP pattern selectivity among all SH3 families. To evaluate the binding capabilities and affinities, we conducted fluorescence dot blot and polarization experiments using 25 representative SH3 domains and various PRPs derived from SOS1. Our analysis has identified 45 interacting pairs, with binding affinities ranging from 0.2 to 125 micromolar, out of 300 tested and potential new SH3 domain-SOS1 interactions. Furthermore, it establishes a framework to bridge the gap between SH3 and PRP interactions and provides predictive insights into the potential interactions of SH3 domains with PRMs based on sequence specifications. This novel framework has the potential to enhance the understanding of protein networks mediated by SH3 domain–PRM interactions and be utilized as a general approach for other domain–peptide interactions.
AIBP: A New Safeguard against Glaucomatous Neuroinflammation
Authors: Choi Seunghwan , Choi Soo-Ho , Bastola Tonking , Park Younggun , Oh Jonghyun , Kim Keun-Young , Hwang Sinwoo , Miller Yury I. , Ju Won-Kyu
Published: January 2024

Glaucoma is a group of ocular diseases that cause irreversible blindness. It is characterized by multifactorial degeneration of the optic nerve axons and retinal ganglion cells (RGCs), resulting in the loss of vision. Major components of glaucoma pathogenesis include glia-driven neuroinflammation and impairment of mitochondrial dynamics and bioenergetics, leading to retinal neurodegeneration. In this review article, we summarize current evidence for the emerging role of apolipoprotein A-I binding protein (AIBP) as an important anti-inflammatory and neuroprotective factor in the retina. Due to its association with toll-like receptor 4 (TLR4), extracellular AIBP selectively removes excess cholesterol from the plasma membrane of inflammatory and activated cells. This results in the reduced expression of TLR4-associated, cholesterol-rich lipid rafts and the inhibition of downstream inflammatory signaling. Intracellular AIBP is localized to mitochondria and modulates mitophagy through the ubiquitination of mitofusins 1 and 2. Importantly, elevated intraocular pressure induces AIBP deficiency in mouse models and in human glaucomatous retina. AIBP deficiency leads to the activation of TLR4 in Müller glia, triggering mitochondrial dysfunction in both RGCs and Müller glia, and compromising visual function in a mouse model. Conversely, restoring AIBP expression in the retina reduces neuroinflammation, prevents RGCs death, and protects visual function. These results provide new insight into the mechanism of AIBP function in the retina and suggest a therapeutic potential for restoring retinal AIBP expression in the treatment of glaucoma.
Exploring the Potential of Montmorillonite as an Antiproliferative Nanoagent against MDA-MB-231 and MCF-7 Human Breast Cancer Cells
Authors: Sabzevari Alireza Ghannad , Sabahi Hossein , Nikbakht Mohsen , Azizi Mehdi , Dianat-Moghadam Hassan , Amoozgar Zohreh
Published: January 2024

Unlike MCF-7 cells, MDA-MB-231 cells are unresponsive to hormone therapy and often show resistance to chemotherapy and radiotherapy. Here, the antiproliferative effect of biocompatible montmorillonite (Mt) nanosheets on MDA-MB-231 and MCF-7 human breast cancer cells was evaluated by MTT assay, flow cytometry, and qRT-PCR. The results showed that the Mt IC50 for MDA-MB-231 and MCF-7 cells in a fetal bovine serum (FBS)-free medium was ~50 and ~200 µg/mL, and in 10% FBS medium ~400 and ~2000 µg/mL, respectively. Mt caused apoptosis in both cells by regulating related genes including Cas-3, P53, and P62 in MDA-MB-231 cells and Bcl-2, Cas-8, Cas-9, P53, and P62 in MCF-7 cells. Also, Mt arrested MCF-7 cells in the G0/G1 phase by altering Cyclin-D1 and P21 expression, and caused sub-G1 arrest and necrosis in both cells, possibly through damaging the mitochondria. However, fewer gene expression changes and more sub-G1 arrest and necrosis were observed in MDA-MB-231 cells, confirming the higher vulnerability of MDA-MB-231 cells to Mt. Furthermore, MDA-MB-231 cells appeared to be much more vulnerable to Mt compared to other cell types, including normal lung fibroblast (MRC-5), colon cancer (HT-29), and liver cancer (HepG2) cells. The higher vulnerability of MDA-MB-231 cells to Mt was inferred to be due to their higher proliferation rate. Notably, Mt cytotoxicity was highly dependent on both the Mt concentration and serum level, which favors Mt for the local treatment of MDA-MB-231 cells. Based on these results, Mt can be considered as an antiproliferative nanoagent against MDA-MB-231 cells and may be useful in the development of local nanoparticle-based therapies.
The Importance of the Position of the Nucleus in Drosophila Oocyte Development
Authors: Lepesant Jean-Antoine , Roland-Gosselin Fanny , Guillemet Clémentine , Bernard Fred , Guichet Antoine
Published: January 2024

Oogenesis is a developmental process leading to the formation of an oocyte, a haploid gamete, which upon fertilisation and sperm entry allows the male and the female pronuclei to fuse and give rise to a zygote. In addition to forming a haploid gamete, oogenesis builds up a store of proteins, mRNAs, and organelles in the oocyte needed for the development of the future embryo. In several species, such as Drosophila, the polarity axes determinants of the future embryo must be asymmetrically distributed prior to fertilisation. In the Drosophila oocyte, the correct positioning of the nucleus is essential for establishing the dorsoventral polarity axis of the future embryo and allowing the meiotic spindles to be positioned in close vicinity to the unique sperm entry point into the oocyte.
Exploring the Influence of the Deposition Parameters on the Properties of NiTi Shape Memory Alloy Films with High Nickel Content
Authors: Fontes André V. , Freitas Rodrigues Patrícia , Santo Daniela , Ramos Ana Sofia
Published: January 2024

NiTi shape memory alloy films were prepared by magnetron sputtering using a compound NiTi target and varying deposition parameters, such as power density, pressure, and deposition time. To promote crystallization, the films were heat treated at a temperature of 400 °C for 1 h. For the characterization, scanning electron microscopy, energy dispersive X-ray spectroscopy, atomic force microscopy, synchrotron X-ray diffraction, and nanoindentation techniques were used on both as-deposited and heat-treated films. Apart from the morphology and hardness of the as-deposited films that depend on the deposition pressure, the power applied to the target and the deposition pressure did not seem to significantly influence the characteristics of the NiTi films studied. After heat treatment, austenitic (B2) crystalline superelastic films with exceptionally high nickel content (~60 at.%) and vein-line cross-section morphology were produced. The crystallization of the films resulted in an increase in hardness, Young’s modulus, and elastic recovery.
Mutual Impact of Four Organic Calcium Salts on the Formation and Properties of Micro-Arc Oxidation Coatings on AZ31B Magnesium Alloys
Authors: Chen Changtian , Shi Xiaoting , Zhang Shufang , Shen Youliang , Zhao Ying , Zhang Rongfa , Zhao Rongfang
Published: January 2024

Calcium phosphate (Ca–P) coatings provide an effective approach in current research and the clinical application of Mg alloys by endowing them with improved corrosion resistance, biocompatibility, and even bioactivity. Ca-containing coatings were prepared on AZ31B magnesium alloys using the micro-arc oxidation (MAO) technique and a combination of ethylenediaminetetraacetic acid calcium disodium (EDTA–Ca), calcium glycerophosphate (GP–Ca), calcium gluconate (CaGlu2), and calcium lactate (CaLac2) as the Ca source in a near-neutral solution. The respective and mutual impacts of the four calcium salts on the formation and properties of the coatings were investigated. Experimental results indicated that GP–Ca was more decisive than EDTA–Ca, CaGlu2, and CaLac2 in the formation, morphology, and, therefore, the corrosion resistance of the coatings. GP–Ca alone could not effectively incorporate Ca2+ ions into the coatings but it could combine with EDTA–Ca, CaGlu2, and CaLac2 to bring a synergistic effect in improving the Ca content of the coatings. The bifunctional structure of CaGlu2 and CaLac2, containing hydroxyl groups and carboxylic groups with anchoring effects, enabled them to enhance the Ca content of the coatings. However, due to minor differences in functional group orientation, CaGlu2 was a little more efficient than CaLac2 in increasing Ca content, while CaLac2 was a little more efficient than CaGlu2 in improving the corrosion resistance of the coatings. Finally, the total concentration of the four calcium salts, [Ca2+]T, should be controlled at a proper level; otherwise, excessively high [Ca2+]T would produce localized microbumps originating from coating ablation, eventually deteriorating the corrosion resistance of the coatings.
Formation and Electrochemical Properties of Heterostructured Electrodes Based on Cu2O and CuCo2O4
Authors: Murashkina Anna A. , Rudakova Aida V. , Bakiev Tair V. , Emeline Alexei V. , Bahnemann Detlef W.
Published: January 2024

Individual (FTO/Cu2O and FTO/CuCo2O4) and heterostructured (FTO/BiVO4/Cu2O, FTO/BiVO4/CuCo2O4, and FTO/CuCo2O4/Cu2O) electrodes were successfully formed using the electrodeposition method on copper-containing compounds. The morphology of the synthesized electrode systems, which affect the electrochemical properties, was determined. A comparative study of the electrochemical and photoelectrochemical properties of the individual and heterostructured electrodes showed that the modification of the BiVO4 electrode surface with Cu2O and CuCo2O4 oxides led to a significant increase in its efficiency as a photoanode. The deposition of Cu2O nanoclusters onto CuCo2O4 nanoflakes increased the electrochemical stability of the electrode while maintaining its high capacitance.
Composite Right/Left-Handed Leaky-Wave Antenna with Electrical Beam Scanning Using Thin-Film Ferroelectric Capacitors
Authors: Platonov Roman , Altynnikov Andrey , Komlev Andrey , Tumarkin Andrey , Kozyrev Andrey
Published: January 2024

This article presents a wide-angle-scanning leaky-wave antenna (LWA) based on a composite right/left-handed (CRLH) transmission line. In contrast to traditional semiconductor elements, thin-film ferroelectric capacitors were implemented in the CRLH unit cells to enable electric beam scanning. The proposed CRLH LWA has a single-layer design without metalized vias and is compatible with PCB and thin-film technologies. To fabricate the CRLH LWA prototype, dielectric material substrates and thin-film ferroelectric capacitors were manufactured, and their characteristics were investigated. Double-sided metalized fluoroplast-4 reinforced with fiberglass with a permittivity of 2.5 was used as a substrate for CRLH LWA prototyping. A solid solution of barium strontium titanate (BaxSr1−xTiO3) with a composition of x=0.3 was used as a ferroelectric material in electrically tunable capacitors. The characteristics of the manufactured ferroelectric thin-film capacitors were measured at a frequency of 1 GHz using the resonance method. The capacitors have a tunability of about two and a quality factor of about 50. The antenna prototype consists of ten units with a total length of 1.25 wavelengths at the operating frequency of close to 2.4 GHz. The experimental results demonstrate that the main beam can be shifted within the range of −40 to 16 degrees and has a gain of up to 3.2 dB. The simple design, low cost, and excellent wide-angle scanning make the proposed CRLH LWA viable in wireless communication systems.
Evaluation of the Wear Properties and Corrosion Resistance of 52100 Steel Coated with Ni/CrC by Cold Spraying
Authors: Lupu Fabian , Munteanu Corneliu , Müftü Sinan , Benchea Marcelin , Cimpoesu Ramona , Ferguson Gehn , Boese Sam , Schwartz Patricia , Istrate Bogdan , Arsenoaia Vlad
Published: January 2024

Coatings deposited by the cold particle gas spray method have shown significant potential for enhancing the properties of metals. We investigated the wear characteristics and corrosion behavior of 52100 steel coated with a mixture of Ni/chromium carbide (Ni/CrC) particles. These coatings exhibited high density and were devoid of cracks, closely adhering to the substrate’s surface. Microscratch resistance testing revealed strong resilience, with the apparent friction coefficient exhibiting multiple peaks as the displacement varied. The determination of the friction coefficient, utilizing linear and rotational sliding tests, displayed a brief transition period. This occurred as the apparent contact area expanded until it reached an equilibrium state, with the large asperities being smoothed out and the remaining particles on the sliding track acting as an abrasive material, resulting in higher friction coefficient values. Electro-corrosion tests confirmed the near-intact condition of the deposited layer. Few compounds were detected in the electrolyte solution, resulting in significantly lower oxidation in the layer compared to the base material.
Overview of Surface Modification Techniques for Titanium Alloys in Modern Material Science: A Comprehensive Analysis
Authors: Gao Kang , Zhang Yun , Yi Junhao , Dong Fang , Chen Pinghu
Published: January 2024

Titanium alloys are acclaimed for their remarkable biocompatibility, high specific strength, excellent corrosion resistance, and stable performance in high and low temperatures. These characteristics render them invaluable in a multitude of sectors, including biomedicine, shipbuilding, aerospace, and daily life. According to the different phases, the alloys can be broadly categorized into α-titanium and β-titanium, and these alloys demonstrate unique properties shaped by their respective phases. The hexagonal close-packed structure of α-titanium alloys is notably associated with superior high-temperature creep resistance but limited plasticity. Conversely, the body-centered cubic structure of β-titanium alloys contributes to enhanced slip and greater plasticity. To optimize these alloys for specific industrial applications, alloy strengthening is often necessary to meet diverse environmental and operational demands. The impact of various processing techniques on the microstructure and metal characteristics of titanium alloys is reviewed and discussed in this research. This article systematically analyzes the effects of machining, shot peening, and surface heat treatment methods, including surface quenching, carburizing, and nitriding, on the structure and characteristics of titanium alloys. This research is arranged and categorized into three categories based on the methods of processing and treatment: general heat treatment, thermochemical treatment, and machining. The results of a large number of studies show that surface treatment can significantly improve the hardness and friction mechanical properties of titanium alloys. At present, a single treatment method is often insufficient. Therefore, composite treatment methods combining multiple treatment techniques are expected to be more widely used in the future. The authors provide an overview of titanium alloy modification methods in recent years with the aim of assisting and promoting further research in the very important and promising direction of multi-technology composite treatment.
The Preparation and Properties of Thick Bronze Coating Using Friction-Assisted Jet Electrodeposition
Authors: Yao Yilin , Cheng Jihua , Ji Hai , Zhang Shiji , Pang Haozhe , Chen Ya , Tian Zongjun
Published: January 2024

In this study, a novel friction-assisted jet electrodeposition technology was applied to prepare bronze coating on 40CrNiMoA structural steel surfaces. The bronze electrode was designed with internal flow channels and nozzles, and the friction brushes made of alumina and silicon carbide were connected to the electrode surface. It was reported that the quality and deposition rate of the thick bronze coatings were significantly improved with friction-assisted jet electrodeposition. The roughness and microstructures were refined, and the deposition rate was up to 100 μm/h when the current density was 8 A/dm2. In addition, the chemical composition was related to current density as the content of tin in the bronze coating made with FJED decreased at a high current density. Moreover, the grain structures were α-CuSn in the solid-solution phase and the average grain size of FJED coatings was refined at a current density of 8 A/dm2.
An Interactive Training Model for Myoelectric Regression Control Based on Human–Machine Cooperative Performance
Authors: Igual Carles , Castillo Alberto , Igual Jorge
Published: January 2024

Electromyography-based wearable biosensors are used for prosthetic control. Machine learning prosthetic controllers are based on classification and regression models. The advantage of the regression approach is that it permits us to obtain a smoother and more natural controller. However, the existing training methods for regression-based solutions is the same as the training protocol used in the classification approach, where only a finite set of movements are trained. In this paper, we present a novel training protocol for myoelectric regression-based solutions that include a feedback term that allows us to explore more than a finite set of movements and is automatically adjusted according to real-time performance of the subject during the training session. Consequently, the algorithm distributes the training time efficiently, focusing on the movements where the performance is worse and optimizing the training for each user. We tested and compared the existing and new training strategies in 20 able-bodied participants and 4 amputees. The results show that the novel training procedure autonomously produces a better training session. As a result, the new controller outperforms the one trained with the existing method: for the able-bodied participants, the average number of targets hit is increased from 86% to 95% and the path efficiency from 40% to 84%, while for the subjects with limb deficiencies, the completion rate is increased from 58% to 69% and the path efficiency from 24% to 56%.
Multiclass AI-Generated Deepfake Face Detection Using Patch-Wise Deep Learning Model
Authors: Arshed Muhammad Asad , Mumtaz Shahzad , Ibrahim Muhammad , Dewi Christine , Tanveer Muhammad , Ahmed Saeed
Published: January 2024

In response to the rapid advancements in facial manipulation technologies, particularly facilitated by Generative Adversarial Networks (GANs) and Stable Diffusion-based methods, this paper explores the critical issue of deepfake content creation. The increasing accessibility of these tools necessitates robust detection methods to curb potential misuse. In this context, this paper investigates the potential of Vision Transformers (ViTs) for effective deepfake image detection, leveraging their capacity to extract global features. Objective: The primary goal of this study is to assess the viability of ViTs in detecting multiclass deepfake images compared to traditional Convolutional Neural Network (CNN)-based models. By framing the deepfake problem as a multiclass task, this research introduces a novel approach, considering the challenges posed by Stable Diffusion and StyleGAN2. The objective is to enhance understanding and efficacy in detecting manipulated content within a multiclass context. Novelty: This research distinguishes itself by approaching the deepfake detection problem as a multiclass task, introducing new challenges associated with Stable Diffusion and StyleGAN2. The study pioneers the exploration of ViTs in this domain, emphasizing their potential to extract global features for enhanced detection accuracy. The novelty lies in addressing the evolving landscape of deepfake creation and manipulation. Results and Conclusion: Through extensive experiments, the proposed method exhibits high effectiveness, achieving impressive detection accuracy, precision, and recall, and an F1 rate of 99.90% on a multiclass-prepared dataset. The results underscore the significant potential of ViTs in contributing to a more secure digital landscape by robustly addressing the challenges posed by deepfake content, particularly in the presence of Stable Diffusion and StyleGAN2. The proposed model outperformed when compared with state-of-the-art CNN-based models, i.e., ResNet-50 and VGG-16.
A Critical Review of Anti-Corrosion Chemical Surface Treatment of Aluminum Alloys Used for Sports Equipment
Authors: Leng Bo , Xue Yuhua , Li Jing , Qi Jiantao , Yi Aihua , Zhao Qixin
Published: January 2024

Aluminum alloys with low-weight property are promising structure materials for sports equipment. Alloying element-rich second-phase particles create the risk of localized corrosion and result in failure of sports equipment. Chromate conversion coatings as conventional and successful surface treatments were employed to provide a thin but compact film against corrosion. However, chromate species were toxic and carcinogenic for human beings and this process has been highly restricted. In this sense, alternative processes such as trivalent chromium conversion coating with low environmental risk require better corrosion-resistant performance compared to chromate conversion coating. In addition, the closed-loop system of the chromate electroplating process has been used in Europe and the United States. This is also a sustainable process for surface treatment of aluminum alloys applied in sports equipment. The present paper aims to summarize the methods and types of different aluminum alloy surface treatments and compiles the effects of various surface treatments on the corrosion resistance of aluminum alloys. The eco-friendly application of aluminum alloys in the field of sports equipment may be facilitated in the future.
In-Gap Edge and Domain-Wall States in Largely Perturbed Phononic Su–Schrieffer–Heeger Lattices
Authors: Rajabpoor Alisepahi Amir , Ma Jihong
Published: January 2024

Topological states of matter have attracted significant attention due to their intrinsic wave-guiding and localization capabilities robust against disorders and defects in electronic, photonic, and phononic systems. Despite the above topological features that phononic crystals share with their electronic and photonic counterparts, finite-frequency topological states in phononic crystals may not always survive. In this work, we discuss the survivability of topological states in Su–Schrieffer–Heeger models with both local and non-local interactions and larger symmetry perturbation. Although such a discussion is still about ideal mass-spring models, the insights from this study set the expectations for continuum phononic crystals, which can further instruct the application of phononic crystals for practical purposes.
Microstructure and Properties Variation of High-Performance Grey Cast Iron via Small Boron Additions
Authors: Klančnik Grega , Burja Jaka , Klančnik Urška , Šetina Batič Barbara , Krajnc Luka , Resnik Andrej
Published: January 2024

A study was undertaken to investigate the effects of small boron additions on the solidification and microstructure of hypo-eutectic alloyed grey cast iron. The characteristic temperatures upon crystallisation of the treated metal melt were recorded, specifically those concerning small boron addition by using thermal analysis with the ATAS system. Additionally, a standardised wedge test was set to observe any changes in chill performance. The microstructures of thermal analysis samples were analysed using a light optical microscope (LOM) and field emission scanning electron microscopy (FE-SEM) equipped with energy dispersive spectroscopy (EDS), which reveal variations in graphite count number with the addition of boron within observed random and undercooled flake graphite. The effect of boron was estimated by the classical analytical and statistical approach. The solidification behaviour under equilibrium conditions was predicted by a thermodynamic approach using Thermo-Calc. Based on all gathered data, a response model was set with boron for given melt quality and melt treatment using the experimentally determined data. The study reveals that boron as a ferrite and carbide-promoting element under the experimental set shows weak nucleation potential in synergy with other heterogenic nuclei at increased solidification rates, but no considerable changes were observed by the TA samples solidified at slower cooling rates, indicating the loss of the overall inoculation effect. The potential presence of boron nitride as an inoculator for graphite precipitation for a given melt composition and melt treatment was not confirmed in this study. It seems that boron at increased solidification rates can contribute to overall inoculation, but at slower cooling rates these effects are gradually lost. In the last solidification range, an increased boron content could have a carbide forming nature, as is usually expected. The study suggests that boron in traces could affect the microstructure and properties of hypo-eutectic alloyed grey cast iron.
Trichoderma Diversity in Mexico: A Systematic Review and Meta-Analysis
Authors: Ahedo-Quero Hector Osvaldo , Aquino-Bolaños Teodulfo , Ortiz-Hernández Yolanda Donají , García-Sánchez Edgar
Published: January 2024

Trichoderma is a genus of cosmopolitan fungi with more than 375 species described today. Despite its global significance in agriculture, ecosystems, and industry, few studies have focused on studying the diversity and distribution of this genus in Mexico. In this systematic review and meta-analysis, we aimed to understand the diversity and distribution of Trichoderma species in Mexico, both in ecosystems and agroecosystems. For this systematic review, we used the PRISMA methodology. We reviewed forty-one scientific articles, two book chapters, and the GBIF database. We recorded a total of 1082 isolates, revealing the presence of 57 species of the genus Trichoderma in 29 states of Mexico. We found that species from the genus Trichoderma were reported in 20 agroecosystems and 6 ecosystems. T. harzianum was the predominant species in both agricultural and undisturbed soil. Tabasco and Veracruz were the states with the highest species diversity, with 20 and 14 species reported, respectively. Chiapas had the highest diversity indices (Menhinick had 3.20, Simpson had 0.89, and Margalef had 4.16). The coffee crop was the agroecosystem with the highest diversity, with 12 species reported. In the undisturbed ecosystems, tropical rainforests featured 12 different species. This study highlights the distribution of the genus Trichoderma as a cosmopolitan genus. We argue for the importance of the species that comprise the genus and its applications for social benefits.
Diet Composition Explains Interannual Fluctuations in Reproductive Performance in a Lowland Golden Eagle Population
Authors: Sein Gunnar , Väli Ülo
Published: January 2024

Food supply stands out as one of the most critical drivers of population demographics by limiting reproductive rates. In this study, we assessed fluctuations in diet composition and monitored various reproductive parameters over a nine-year period in a Golden Eagle population in an Eastern European peatland. The identification of 2439 prey specimens from 84 species revealed that the studied eagles primarily preyed upon birds (constituting 78.3% of prey numbers and 67.2% of prey biomass) and less on mammals (21.6% and 32.8%, respectively). Grouse emerged as the most important prey group (31% and 27%), followed by waterfowl (17%) and hares (8% and 14%). The most significant prey species, both in terms of numbers and weight, were the Black Grouse, Mountain Hare, Common Crane, and Capercaillie. The share of the Black Grouse decreased, while those of the White-fronted Goose, Roe Deer, and hares increased. The food niche, as measured by the Levins’ index, was broad (6.6), and it expanded during the study. On average, 58.3% of Golden Eagle pairs initiated breeding annually, with 69.1% successfully completing it, and 0.41 young per occupied territory were produced annually; there was pronounced interannual variation in reproductive performance. These fluctuations were associated with the shares of White-fronted Goose, Capercaillie, Mountain Hare and Roe Deer in the diet, suggesting that these species may be gaining increasing importance for the Golden Eagle, particularly following the decline of the Black Grouse.
Microplastic Pollution in Water Systems: Characteristics and Control Methods
Authors: Ma Hao , Chao Liqiang , Wan Hang , Zhu Qin
Published: January 2024

Microplastics have been widely detected in the natural water environment, which brings inevitable risks to the water ecosystem and human health. However, the understanding of the potential impact of microplastics on aquatic animals, plants, and human health is still limited, and technical methods to control microplastic pollution in natural water are still rare. Hence, this paper summarizes the progress of research on microplastic pollution in water systems in terms of microplastic source, attributes, distribution characteristics, environmental effects, and prevention and control methods according to the relevant research reports on water microplastic pollution. It also expounds the basic ways for the prevention, control, and treatment of water microplastics, and looks forward to the research direction of water microplastic pollution in the future. The results show that the abundance of fresh water microplastics in China is higher than that in other regions, but the pollution level of marine microplastics is at the middle level. Compared with other countries, the pollution degree of microplastics in aquatic organisms in China is at the middle and lower level, but the spatial heterogeneity is more obvious. Through hydraulic control and the substitution of degradable plastic products, water microplastic pollution can be greatly reduced. This paper can provide a reference basis for the formulation of microplastic pollution prevention and control in China.
Evaluating the Ranking of Performance Variables in Flexible Manufacturing System through the Best-Worst Method
Authors: Bagherian Anthony , Chauhan Gulshan , Srivastav Arun Lal , Kumar Sharma Rajiv
Published: January 2024

Flexible Manufacturing Systems (FMSs) provide a competitive edge in the ever-evolving manufacturing landscape, offering the agility to swiftly adapt to changing customer demands and product lifecycles. Nevertheless, the complex and interconnected nature of FMSs presents a distinct challenge: the evaluation and prioritization of performance variables. This study clarifies a conspicuous research gap by introducing a pioneering approach to evaluating and ranking FMS performance variables. The Best-Worst Method (BWM), a multicriteria decision-making (MCDM) approach, is employed to tackle this challenge. Notably, the BWM excels at resolving intricate issues with limited pairwise comparisons, making it an innovative tool in this context. To implement the BWM, a comprehensive survey of FMS experts from the German manufacturing industry was conducted. The survey, which contained 34 key performance variables identified through an exhaustive literature review and bibliometric analysis, invited experts to assess the variables by comparing the best and worst in terms of their significance to overall FMS performance. The outcomes of the BWM analysis not only offer insights into the factors affecting FMS performance but, more importantly, convey a nuanced ranking of these factors. The findings reveal a distinct hierarchy: the “Quality (Q)” factor emerges as the most critical, followed by “Productivity (P)” and “Flexibility (F)”. In terms of contributions, this study pioneers a novel and comprehensive approach to evaluating and ranking FMS performance variables. It bridges an evident research gap and contributes to the existing literature by offering practical insights that can guide manufacturing companies in identifying and prioritizing the most crucial performance variables for enhancing their FMS competitiveness. Our research acknowledges the potential introduction of biases through expert opinion, delineating the need for further exploration and comparative analyses in diverse industrial contexts. The outcomes of this study bear the potential for cross-industry applicability, laying the groundwork for future investigations in the domain of performance evaluation in manufacturing systems.
Over-Inflating a Tracheostomy Tube Cuff for Tracheo-Innominate Artery Fistula
Authors: Hsieh Yi-Chia , Tang Wen-Ruei , Chen Ying-Yuan
Published: January 2024

We report an angiographic image of a 58-year-old woman with profuse bleeding from a tracheo-innominate artery fistula. It may not have been possible to obtain this valuable image if adequate initial resuscitation and an over-inflated tracheostomy tube cuff had not been administered to stop bleeding during an emergency.
Real-Time Tracheal Ultrasound vs. Capnography for Intubation Confirmation during CPR Wearing a Powered Air-Purifying Respirator in COVID-19 Era
Authors: Eun Seungwan , Yoon Hee , Kang Soo Yeon , Jo Ik Joon , Heo Sejin , Chang Hansol , Lee Guntak , Park Jong Eun , Kim Taerim , Lee Se Uk , Hwang Sung Yeon , Baek Sun-Young
Published: January 2024

This study aimed to compare the accuracy of real-time trans-tracheal ultrasound (TTUS) with capnography to confirm intubation in cardiopulmonary resuscitation (CPR) while wearing a powered air-purifying respirator (PAPR). This setting reflects increased caution due to contagious diseases. This single-center, prospective, comparative study enrolled patients requiring CPR while wearing a PAPR who visited the emergency department of a tertiary medical center from December 2020 to August 2022. A physician performed the TTUS in real time and recorded the tube placement assessment. Another healthcare provider attached waveform capnography to the tube and recorded end-tidal carbon dioxide (EtCO2) after five ventilations. The accuracy and agreement of both methods compared with direct laryngoscopic visualization of tube placement, and the time taken by both methods was evaluated. Thirty-three patients with cardiac arrest were analyzed. TTUS confirmed tube placement with 100% accuracy, sensitivity, and specificity, whereas capnography demonstrated 97% accuracy, 96.8% sensitivity, and 100% specificity. The Kappa values for TTUS and capnography compared to direct visualization were 1.0 and 0.7843, respectively. EtCO2 was measured in 45 (37–59) seconds (median (interquartile range)), whereas TTUS required only 12 (8–23) seconds, indicating that TTUS was significantly faster (p < 0.001). No significant correlation was found between the physician’s TTUS proficiency and image acquisition time. This study demonstrated that TTUS is more accurate and faster than EtCO2 measurement for confirming endotracheal tube placement during CPR, particularly in the context of PAPR usage in pandemic conditions.