bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Rail Pressure Stability in an Electronically Controlled High-Pressure Common Rail Fuel Injection System via GT-Suite Simulation
Authors: Jiang Hongfeng , Li Zhejun , Jiang Feng , Zhang Shulin , Huang Yan , Hu Jie
Published: January 2025

The high-pressure common rail (HPCR) injection system, a key technology for enhancing diesel engine performance, plays a decisive role in ensuring fuel injection precision and combustion efficiency through rail pressure stability. This study establishes a coupled simulation model of an electronically controlled HPCR injection system and a diesel engine, using GT-Suite to systematically investigate the effects of fuel supply pressure, camshaft speed, high-pressure pump plunger parameters, and inlet and outlet valve characteristics on rail pressure fluctuations. Gray relational analysis quantifies the correlation between these factors and rail pressure variations. The results demonstrate that increasing camshaft speed, injection pulse width, plunger mass, plunger length, plunger spring preload, inlet valve spring preload, and outlet valve body mass reduces rail pressure fluctuations, while variations in fuel supply pressure, plunger spring stiffness, and valve spring stiffness have minimal impact. Notably, the influence of outlet valve spring preload, inlet valve spring stiffness, and inlet valve body mass on rail pressure is nonlinear, with optimal values observed. Gray relational analysis further identifies inlet valve spring preload as having the highest correlation with rail pressure fluctuations (0.815), followed by inlet valve spring stiffness (0.625), with outlet valve spring preload (0.551) and stiffness (0.527) showing relatively lower correlations. This study provides valuable insights for optimizing the HPCR injection system design and contributes to advancements in diesel engine technology.
Analysis of the Relationships Among the Value, Benefit, and Activities of Forest Culture in Korea: An Application of Means-Chain Value Theory
Authors: Chae Jinhae , Kim Seonghak , Choi Nakmin , Kim Taekwon
Published: January 2025

This study explores the relationship between forest culture (FC) and sustainable consumption by applying the means-end chain (MEC) theory. Compared with general products, FC products are consumed from a value consumption perspective, and their benefits have varying impacts on individuals and society. This study uses MEC theory to link the preferred attributes of FC with the expected benefits and pursued values (PVs) of FC. We (1) identified the indicators of the expected benefits of PVs and preferred activities (PAs) of FC through factor analysis, (2) examined the relationships between these factors using MEC theory, and (3) validated the factors through structural equation modeling (SEM). We surveyed 1700 Koreans to explore how FC benefits, values, and activities relate to consumer behavior. Factor analysis divided PVs into symbolic, social, and consumption values and PAs into tourism–exploration, cultural–artistic, and living–leisure activities of FC. According to SEM analysis, the contributing characteristics of FC affect the PVs and, in turn, the PAs of FC, yielding an acceptable model fit (GFI > 0.9). Thus, the concrete attitudes of consumers toward FC were categorized via abstract concepts, which influenced their practical and behavioral attitudes. In conclusion, FC products should be developed with a focus on value consumption.
Spatial Distribution of Critically Endangered Hopea chinensis Plant Seedlings and Relationships with Environmental Factors
Authors: Huang Fang , Xiao Yufei , Wang Renjie , Jiang Ying , Fan Rongyuan , Liu Xiongsheng
Published: January 2025

Hopea chinensis is a representative tree species in evergreen monsoon forests in the northern tropics, but it is currently in a critically endangered state due to destruction by human activities and habitat loss. In this study, we measured and analyzed the number of regenerating seedlings and habitat factors in wild populations of H. chinensis by combining field surveys with laboratory analysis. The aim of this study was to clarify the spatial distribution of H. chinensis seedlings and related factors to provide a scientific basis for conserving its germplasm resources and population restoration. In six populations, most size-class seedlings had aggregated distributions at three scales, and the intensity of aggregation decreased as the sample plot scale increased for most size-class seedlings. In the northern foothills of the Shiwandashan Mountains, size class I seedlings tended to be distributed in habitats with a higher rock bareness rate, whereas size class II and III seedlings tended to be distributed in habitats with a higher canopy density, thicker humus layers, and higher soil moisture content. In the southern foothills of the Shiwandashan Mountains, size class I and II seedlings tended to be distributed in habitats with higher available nitrogen contents, and size class III seedlings tended to be distributed in habitats with higher available nitrogen and soil moisture contents. Therefore, in the southern foothills of the Shiwandashan Mountains, the survival rate of H. chinensis seedlings can be improved by artificially adding soil to increase the thickness of the soil layer in stone crevices and grooves, regularly watering the seedlings during the dry season, and appropriately reducing the coverage of the shrub layer. In the northern foothills, the survival rate of H. chinensis seedlings can be enhanced by regularly applying nitrogen fertilizer and watering to increase the available nitrogen and soil moisture contents.
Responses of the Leaf Traits of Ten Common Green Space Plants in Nanjing to Urban Green Space Environments
Authors: Zhang Yuanlan , Ma Chunyan , Lian Rui , Zhang Di , Sheng Qianqian , Zhu Zunling
Published: January 2025

Leaf functional traits are an important part of plant adaptive strategies and respond differently to different environments, but our understanding of how plants adapt to highly complex urban environments through coordinated changes in leaf functional traits is limited. In order to deeply explore the effects of different urban environments on the leaf functional traits of landscape plants, this study focused on ten common landscape plants in Nanjing, specifically, Euonymus japonicus, Pittosporum tobira, Loropetalum chinense, Photinia × fraseri, Acer palmatum, Euonymus japonicus ‘Aurea-marginatus’, Platanus acerifolia, Camphora officinarum, Ginkgo biloba, and Lagerstroemia indica. The study employed one-way analysis of variance (ANOVA), two-way ANOVA, principal component analysis (PCA), and membership function comprehensive evaluation to explore the responses of green space plants to the environments of urban park green spaces (PAR), urban community green spaces (NL), and urban road green spaces (CR) and to analyze the impact of the trade-off strategies of plants in different green space environments based on leaf functional traits. The results showed that plant leaf functional traits and their adaptive strategies varied in different urban environments in Nanjing. The contents of LDMC, MDA, POD, and CAT in the ten plant species followed the order CR > NL > PAR, and SLA, SOD, SS, and total chlorophyll showed trends of PAR > NL > CR. The microstructures of the leaves of the ten plant species, such as SA, TUE, and TS, were evident in the expression of adaptive adjustments to the different green space environments. The principal component analysis and the comprehensive evaluation of the affiliation function of the indicators of the ten plant species revealed that the adaptability of the ten green space plants to the complex urban environment was as follows: Euonymus japonicus ‘Aurea-marginatus’ > Lagerstroemia indica > Acer palmatum > Photinia × fraseri > Platanus acerifolia> Camphora officinarum > Loropetalum chinense > Ginkgo biloba > Euonymus japonicus > Pittosporum tobira. The results of the study will help to further understand the layout, application, and maintenance of landscape plants in the context of urbanization and provide support for related theories.
Global Warming as Revealed Through Thirty Years of Analysis on Breeding of Mediterranean Tits
Authors: Cusimano Camillo A. , Chiarello Sara , Massa Bruno
Published: January 2025

We followed the reproductive parameters of two species of tits, Great and Blue tits, over three decades (1993–2022), in three close habitats of the Mediterranean island of Sicily. We found that they regulated egg-laying dates in the same way, even though they had different dates. The anticipation of laying dates was inversely correlated with the temperature trends of February. The Great tit showed a significant anticipation of laying dates in all three habitats: in the pine woodland, an average anticipation of 19.8 days in 30 years was observed; in the oak woodland, an anticipation of 10.5 days in 30 years was seen; and in the mixed woodland, an anticipation of 13 days in 26 years was seen. The tendency to anticipate the laying date was also observed for the Blue tit in all habitats: in the pine woodland, it significantly anticipated the laying date by 14.4 days in 30 years, while in the oakwood, the anticipation was slightly smaller at 8.7 days in 30 years, and in the mixed woodland, an anticipation of 13.8 days in 26 years was observed. Breeding success did not change over the years for both species. Although we observed through the recoveries of ringed tits a movement of breeding individuals from the pine to the oakwood, we never observed the opposite. This is likely due to the greater availability of feeding resources in the natural woodland. In fact, tits in oak forests feed mainly on caterpillars; in other woodland types, they do not find caterpillars and feed on other arthropods. We hypothesize that most likely in Sicily, these tits, thanks to their phenotypic plasticity, will adapt to the present new conditions resulting from global warming, but if temperatures rise further, they will certainly face difficult times.
Effects of Artemisia argyi Aqueous Extract on Rumen Fermentation Parameters and Microbiota in Lambs
Authors: Gao Ruiheng , Du Juan , Gang Gen , Jin Xiao , Xing Yuanyuan , Xu Yuanqing , Hong Lei , Yan Sumei , Shi Binlin
Published: January 2025

This study sought to evaluate the effects of Artemisia argyi aqueous extract (AAE) on rumen fermentation parameters and the microbiota within the rumen of lambs. A total of 32 lambs that are 3 months old and 24.06 ± 0.04 kg in body weight were randomly assigned to four treatment groups, with eight replicates per treatment. The diets for the four groups were formulated with the following adding dose of AAE: 0 mg/kg (CON), 500 mg/kg (AAE-L), 1000 mg/kg (AAE-M), and 1500 mg/kg (AAE-H), respectively. The results showed that, compared to the CON group, three AAE add groups significantly decreased the A/P ratio; AAE-M and AAE-H groups significantly increased MCP and propionic acid contents. Supplementation with AAE had no significant effect on the alpha and beta diversity of the rumen microbiota, but significantly increased the relative abundances of beneficial bacteria, such as Actinobacteriota in the rumen. In conclusion, AAE supplementation improved the rumen fermentation and microbiota of lambs. In the overall consideration, under the conditions of this research, the supplementation of 1000 mg/kg AAE was optimal.
Genetic Diversity and Population Structure of Commercial Eel Conger myriaster (Anguilliformes: Congridae) Along the Coasts of China Based on Complete Mitochondrial Cyt b Sequences
Authors: Xiao Peiyi , Yang Tianyan
Published: January 2025

To better understand the population genetic structure and molecular biological background of Conger myriaster, an economically important marine fish, a total of 217 complete mitochondrial cytochrome b (Cyt b) gene sequences with a length of 1142 bp were obtained to assess the genetic diversity, population differentiation, and demographic history of seven populations along the coastal waters of China. The analysis of population genetic diversity showed a high level of haplotype diversity and a low level of nucleotide diversity. The analysis of molecular variance (AMOVA) and the genetic differentiation coefficient (FST) showed that most of the variation came from within populations, and the geographic distribution of haplotypes revealed non-significant genetic differentiation among populations. Tracing the population dynamic history, the results of the neutrality test and mismatch analysis suggested that the populations of C. myriaster in coastal China seas had experienced demographic expansion, and the expansion time can be traced back to the middle Pleistocene period. These results provide supplemental information for the sustainable utilization of fishery resources of this species.
Supplementation of Enzymatic Hydrolysate in Low-Fishmeal and Low-Crop Diet Improves Growth, Antioxidant Capacity, and Immunity of Juvenile Sea Cucumber Apostichopus japonicus (Selenka)
Authors: Li Qingfei , Liu Zhengyong , Yang Gang , Zhang Danyang , Qin Huimin , Xia Bin , Liu Shilin , Chen Jinghua
Published: January 2025

As the global demand for aquafeed ingredients continues to rise, sourcing sustainable alternatives is crucial for aquaculture industries. This study aimed to explore the potential of enzymatic hydrolysate as a substitute for traditional fishmeal and soybean meal in diets for juvenile sea cucumbers (Apostichopus japonicus). Three isonitrogenous (15% crude protein) and isolipidic (2.4% crude lipid) diets were formulated: a control diet containing 10% fishmeal and 5% soybean meal and two experimental low-fishmeal (8%) and low-soybean meal (0%) diets, supplemented with either 8% enzymatically hydrolyzed fish stickwater (EFS) or 8% enzymatically hydrolyzed chicken pulp (ECP), designated as Control, EFS, and ECP, respectively. Juvenile sea cucumbers (initial body weight, 0.25 ± 0.01 g) were fed these diets for 84 days to evaluate the effects of ECP and EFS on their growth performance, antioxidant capacity, and inflammatory responses. The results revealed significantly higher final weights and specific growth rates in both experimental groups than the control (p < 0.05). The proximate chemical compositions of sea cucumber were less affected by the diets (p > 0.05). Compared with the control group, significantly elevated levels of digestive enzymes, antioxidants, and lysozyme, together with lower malondialdehyde levels, were recorded in the experimental groups (p < 0.05). ECP appeared to exhibit greater potency than EFS in enhancing growth performance and antioxidant capacity. Similar trends were observed in the mRNA expression of SOD, CAT, and inflammation-related genes across the groups. In a nutshell, both ECP and EFS supplemented in a low-fishmeal and low-soybean meal diet could effectively promote the growth and health of A. japonicus, with ECP showing a superior effect over EFS. These findings suggest that enzymatic hydrolysate demonstrates potential as a viable alternative to traditional fishmeal and soybean meal in diets for sustainable sea cucumber aquaculture. Further investigations are warranted to reveal its underlying mechanism.
Magnetohyrodynamic Turbulence in a Spherical Shell: Galerkin Models, Boundary Conditions, and the Dynamo Problem
Authors: Shebalin John V.
Published: January 2025

The ‘dynamo problem’ requires that the origin of the primarily dipole geomagnetic field be found. The source of the geomagnetic field lies within the outer core of the Earth, which contains a turbulent magnetofluid whose motion is described by the equations of magnetohydrodynamics (MHD). A mathematical model can be based on the approximate but essential features of the problem, i.e., a rotating spherical shell containing an incompressible turbulent magnetofluid that is either ideal or real but maintained in an equilibrium state. Galerkin methods use orthogonal function expansions to represent dynamical fields, with each orthogonal function individually satisfying imposed boundary conditions. These Galerkin methods transform the problem from a few partial differential equations in physical space into a huge number of coupled, non-linear ordinary differential equations in the phase space of expansion coefficients, creating a dynamical system. In the ideal case, using Dirichlet boundary conditions, equilibrium statistical mechanics has provided a solution to the problem. As has been presented elsewhere, the solution also has relevance to the non-ideal case. Here, we examine and compare Galerkin methods imposing Neumann or mixed boundary conditions, in addition to Dirichlet conditions. Any of these Galerkin methods produce a dynamical system representing MHD turbulence and the application of equilibrium statistical mechanics in the ideal case gives solutions of the dynamo problem that differ only slightly in their individual sets of wavenumbers. One set of boundary conditions, Neumann on the outer and Dirichlet on the inner surface, might seem appropriate for modeling the outer core as it allows for a non-zero radial component of the internal, turbulent magnetic field to emerge and form the geomagnetic field. However, this does not provide the necessary transition of a turbulent MHD energy spectrum to match that of the surface geomagnetic field. Instead, we conclude that the model with Dirichlet conditions on both the outer and the inner surfaces is the most appropriate because it provides for a correct transition of the magnetic field, through an electrically conducting mantle, from the Earth’s outer core to its surface, solving the dynamo problem. In addition, we consider how a Galerkin model velocity field can satisfy no-slip conditions on solid boundaries and conclude that some slight, kinetically driven compressibility must exist, and we show how this can be accomplished.
Liquid–Liquid Flow and Mass Transfer Enhancement in Tube-in-Tube Millireactors with Structured Inserts and Advanced Inlet Designs
Authors: Zhu Feng , Pan Xingxing , Cao Xichun , Chen Yandan , Wang Rijie , Lin Jiande , Liu Hanyang
Published: January 2025

Liquid–liquid mass transfer is crucial in chemical processes like extraction and desulfurization. Traditional tube-in-tube millireactors often overlook internal flow dynamics, focusing instead on entry modifications. This study explores mass transfer enhancement through structured inserts (twisted tapes, multi-blades) and inlet designs (multi-hole injectors, T-mixers). Using high-speed imaging and water–succinic acid–butanol experiments, flow patterns and mass transfer rates were analyzed. Results show annular and dispersion flows dominate under tested conditions with structured inserts lowering the threshold for dispersion flow. Multi-hole injectors improved mass transfer by over 40% compared to T-mixers in plain tubes, while C-tape inserts achieved the highest volumetric mass transfer coefficient (2.43 s−1) due to increased interfacial area and droplet breakup from energy dissipation. This approach offers scalable solutions to enhance tube-in-tube millireactor performance for industrial applications.
A Food Pyramid and Nutritional Strategies for Managing Nausea and Vomiting During Pregnancy: A Systematic Review
Authors: Rondanelli Mariangela , Perna Simone , Cattaneo Carlo , Gasparri Clara , Barrile Gaetan Claude , Moroni Alessia , Minonne Leonardo , Lazzarotti Alessandro , Mansueto Francesca , Mazzola Giuseppe
Published: January 2025

Nausea and vomiting during pregnancy (NVP) affect up to 85% of pregnant women and usually begin between the 4th and 7th weeks of gestation, and symptoms often peak around the 9th week and generally resolve by the 20th week in most cases, with severe cases termed hyperemesis gravidarum (HG) impacting physical and psychological health. This review aims to provide a structured dietary approach to managing NVP by developing a food pyramid specifically for this population, based on a systematic evaluation of dietary evidence. The findings highlight the beneficial effects of dietary patterns rich in fruits, vegetables, and protein sources in reducing NVP symptoms. Protein intake is shown to alleviate nausea and vomiting by stabilizing gastric motility and addressing nutritional deficiencies. The review also explores the potential benefits of herbal supplements, like ginger and vitamin B6. By integrating these dietary strategies with pharmacological treatments, a more holistic approach to managing NVP can be achieved, enhancing both maternal well-being and fetal health. The proposed food pyramid emphasizes glycemic stability, hydration, and gradual nutrient intake, offering a structured dietary guide for pregnant women experiencing NVP and HG.
Going Forward to Unveil the Nature of γ Cas Analogs
Authors: Nazé Yaël
Published: January 2025

The star γ Cas and its analogs are a subset of Be stars that display particularly hard and bright thermal X-ray emission, which has no equivalent among other massive stars. Here, I will review their characteristics and present the latest results of our optical and X-ray monitoring campaigns, including an assessment of the links between the circumstellar environment and the high-energy properties. Possible scenarios to explain this phenomenon will be presented in light of these observational results.
Transcriptomic Profiling Highlights Metabolic and Biosynthetic Pathways Involved in In Vitro Flowering in Anoectochilus roxburghii (Wall.) Lindl.
Authors: Yu Shuisheng , Liu Julian , Cai Chenchen , Zhang Yi , Fu Shuangbin , Yang Yanping , Zhou Zhuang , Ying Zhen
Published: January 2025

Background: Tissue culture is one of the most important methods for propagating orchids. Notably, many orchid seedlings exhibit autonomous flowering during the cultivation process. To explore the underlying mechanism, Anoectochilus roxburghii (Wall.) Lindl., an orchid that spontaneously forms in vitro flowers, was analyzed in this study. Methods: Bud samples at the early, middle, and fully open stages were collected for transcriptome sequencing, followed by differential expression, trend, enrichment and protein–protein interaction (PPI) network analyses. Results: Differential gene expression analysis identified 2364, 4137, and 6522 differentially expressed genes (DEGs) in the early vs. middle, middle vs. open, and early vs. open comparisons, respectively. These DEGs were significantly enriched in various metabolic and biosynthetic pathways, particularly in ko01100 (metabolic pathways). PPI network analysis further identified hub genes, including MCM3, MCM4, and MCM7, which are associated with DNA replication, and CURL3, which is linked to plant hormone signal transduction pathways. Conclusion: Our findings provide novel insights into the molecular mechanisms driving in vitro flowering in A. roxburghii, highlighting the importance of metabolic and biosynthetic process signaling in this unique developmental transition. These results provide valuable resources for future studies on orchid propagation and floral development.
Combined Effect of Conventional Chemotherapy with Epigenetic Modulators on Glioblastoma
Authors: Albulescu Adrian , Botezatu Anca , Fudulu Alina , Hotnog Camelia , Bostan Marinela , Mihăilă Mirela , Iancu Iulia , Plesa Adriana , Brasoveanu Lorelei
Published: January 2025

Background/Objectives: Glioblastoma is the most common malignant primary brain tumor, characterized by necrosis, uncontrolled proliferation, infiltration, angiogenesis, apoptosis resistance, and genomic instability. Epigenetic modifiers hold promise as adjuvant therapies for gliomas, with synergistic combinations being explored to enhance efficacy and reduce toxicity. This study aimed to evaluate the effects of single or combined treatments with various anticancer drugs (Carboplatin, Paclitaxel, Avastin), natural compounds (Quercetin), and epigenetic modulators (suberoylanilide hydroxamic acid and 5-Azacytidine) on the expression of some long noncoding RNAs and methylation drivers or some functional features in the U87-MG cell line. Methods: Treated and untreated U87-MG cells were used for the evaluation of drug-induced cytotoxicity, apoptotic events, and distribution in cell cycle phases, detection of cytokine release, and assessment of gene expression and global methylation. Results: Cytotoxicity assays led to the selection of drug concentrations to be used in further experiments. Expression analysis revealed distinct downregulation of nearly all investigated genes and long noncoding RNAs following treatments. All treatments resulted in a higher percentage of global methylation compared to untreated controls. All treatments effectively increased levels of apoptosis, while the epigenetic modulators exhibited a lower proliferation profile, with combined treatments showing elevated values of cell lysis. Conclusions: The results indicate a link between Carboplatin and Avastin treatments and DNA methylation mechanisms involving EZH2, DNMT3A, and DNMT3B, with Avastin’s direct impact on these enzymes warranting further study. This research underscores the promise of platinum-based therapies combined with epigenetic drugs to reactivate silenced tumor suppressor genes and optimize methylation profiles.
The Prevalence of Single Nucleotide Polymorphisms of the AOC1 Gene Associated with Diamine Oxidase (DAO) Enzyme Deficiency in Healthy Newborns: A Prospective Population-Based Cohort Study
Authors: Fortes Marin Emma , Carrera Marcolin Lydia , Martí Melero Laia , Tintoré Gazulla María , Beltran Porres Mireia
Published: January 2025

Background/Objectives: The prevalence of the diamine oxidase (DAO) enzyme deficiency of a genetic origin has not been previously assessed. A prospective population-based study was conducted in a sample of 200 healthy newborns aimed to determine the prevalence of DAO enzyme deficiency caused by single nucleotide polymorphism (SNP) variants of the AOC1 gene. Methods: Genotyping was performed in oral mucosa samples collected around 2 days after birth. The four more frequent SNPs, c.47C>T (rs10156191), c.995C>T (rs1049742), c.1990C>G (rs10449793), and c.691G>T (rs2052129), were analyzed. Results: DAO deficiency was present in 132 newborns, with a prevalence of 66% (95% confidence interval [CI] 59–73%). The rs10449793 variant showed a prevalence of 46%, followed by rs10156191 with a prevalence of 42.5%, and rs2052129 with a prevalence of 39.5%. The variant rs1049742 showed the lowest prevalence (9.5%). The frequency of one, two, three, or four SNPs was 23%, 23.5%, 10.5%, and 9%, respectively. In all fours SNP variants, heterozygous carriers were more frequent than homozygous carriers (19% homozygosity). Differences in the prevalence of DAO deficiency between males (68%, 66/96) and females (63.4%, 66/104) were not found (p = 0.885). The prevalence in Caucasian newborns was 66.5% (123/185), as compared with 60% (9/15) in Latin Americans (p = 0.821). Conclusions: This study carried out in healthy newborns indicates that there is a high prevalence (66%) of DAO deficiency of a genetic origin in the general population.
Biofacies Analysis of Zanclean Sediments in Virginia: Unraveling the Past Through Benthic Foraminifera
Authors: Spivey Whittney
Published: January 2025

Early Pliocene sedimentary deposits are exposed at the surface along the James and York Rivers, across southeastern Virginia. The Zanclean age (5.33–3.60 Ma) Sunken Meadow Member of the Yorktown Formation records a relatively large-scale marine transgression in the Salisbury Embayment. A total of 15 samples were collected from an outcrop near Spring Grove, VA, for grain-size analysis and to document benthic and planktic foraminiferal assemblages. The sediments are generally moderately well-sorted, shelly fine sands. A total of 48 benthic taxa were recorded from the Sunken Meadow Member, though only 14 taxa occurred in proportions high enough to be included in the Q-mode cluster analysis (>3% of the total assemblage). Low numbers of planktic foraminifera indicate relatively shallow water deposition. Biofacies analysis shows three distinct biofacies groups in the Sunken Meadow Member and the benthic foraminiferal community shifts throughout the unit are indicative of changes in nutrient availability, surface productivity, and bottom water oxygenation. The results indicate a middle to outer neritic depositional environment similar to modern conditions found south of Cape Hatteras, NC.
Assessment of Diabetes-Related Knowledge and Dietary Patterns Among Type 2 Diabetes Mellitus Patients in Central Saudi Arabia: Insights for Tailored Health Education Strategies
Authors: Alsaidan Aseel Awad , Alanazi Mohammed Ibrahim , Thirunavukkarasu Ashokkumar
Published: January 2025

Background and Objectives: Understanding the knowledge and dietary patterns of type 2 diabetes mellitus (T2DM) patients is essential to identify gaps and design tailored health education strategies to improve self-management and clinical outcomes. We assessed the diabetes-related dietary patterns, knowledge, and associated factors of T2DM patients. Methods: The study utilized a cross-sectional design, surveying 363 T2DM patients using a validated and pretested questionnaire. Knowledge levels were categorized as low (<50%), medium (50–75%), and high (>75%), and dietary patterns were classified as unhealthy (<34%), moderately healthy (34–67%), and healthy (>67%). We analyzed the data using the Statistical Package for the Social Sciences (SPSS, version 23.0). The authors tested the relationship between diabetes-related dietary patterns and knowledge with Spearman’s analysis. The multivariate regression approach established the factors associated with these two components. Results: The findings revealed that 36.4% of participants had low knowledge, 34.4% had medium knowledge, and only 29.2% demonstrated high knowledge. Regarding dietary patterns, 34.4% were categorized as unhealthy, 33.3% as moderately healthy, and 32.3% as healthy. The authors found that these two domains were positively correlated (rho = 0.649, p = 0.001). Diabetes-related knowledge was significantly associated with gender (p = 0.018), duration of diabetes (p = 0.001), and patients with a family history of T2DM (p = 0.007). The dietary pattern was significantly associated with age (p = 0.001), duration of diabetes (p = 0.032), and presence of other chronic diseases (p = 0.028). Conclusions: The findings underscore the need for targeted health education strategies that address gaps in dietary knowledge and promote healthier eating behaviors among T2DM patients in Central Saudi Arabia.
Development of Sheep Intestinal Organoids for Studying Deoxynivalenol-Induced Toxicity
Authors: Wang Hongyu , He Xige , Zhang Miaomiao , Fan Na , Yang Zongxuan , Shen Ting , Guo Jiaojiao , Song Yongli , Cao Guifang , Liu Yongbin , Li Xihe , Nashun Buhe
Published: January 2025

Sheep are an important livestock species whose gastrointestinal tract is essential for overall health. Feed contaminants such as bacterial toxins and mycotoxins severely damage the sheep intestine, yet the mechanisms remain mostly elusive partially due to the lack of physiologically relevant in vitro models. Here, we investigated molecular mechanisms underlying deoxynivalenol (DON)-induced toxicity by developing intestinal organoids from isolated intestinal crypts of Hu sheep. The organoids had a central lumen and monolayer epithelium, and could be continuously passaged, cryopreserved, and resuscitated. Histological and transcriptomic analysis showed that the intestinal organoids recapitulate the cell lineages and gene expression characteristics of the original intestinal tissues. Statistical analysis indicated that DON exposure significantly inhibited organoid formation efficiency, as well as the proliferation and activity of intestinal organoid cells. RNA-seq and Western blotting analysis further revealed that DON exposure induces intestinal toxicity by inhibiting the PI3K/AKT/GSK3β/β-catenin signaling pathway. Our study provides a novel example of organoid application in toxicity studies and reveals the signaling pathway involved in DON-induced toxicity in sheep, which is of great significance for improving mitigation strategies for DON.
The Role of Visual Electrophysiology in Systemic Hereditary Syndromes
Authors: Yu Minzhong , Vieta-Ferrer Emile R. , Bakdalieh Anas , Tsai Travis
Published: January 2025

Visual electrophysiology is a valuable tool for evaluating the visual system in various systemic syndromes. This review highlights its clinical application in a selection of syndromes associated with hearing loss, mitochondrial dysfunction, obesity, and other multisystem disorders. Techniques such as full-field electroretinography (ffERG), multifocal electroretinography (mfERG), pattern electroretinography (PERG), visual evoked potentials (VEP), and electrooculography (EOG) offer insights into retinal and optic nerve function, often detecting abnormalities before clinical symptoms manifest. In hearing loss syndromes like Refsum disease, Usher syndrome (USH), and Wolfram syndrome (WS), electrophysiology facilitates the detection of early retinal changes that precede the onset of visual symptoms. For mitochondrial disorders such as maternally-inherited diabetes and deafness (MIDD), Kearns–Sayre syndrome (KSS), and neuropathy, ataxia, and retinitis pigmentosa (NARP) syndrome, these tests can be useful in characterizing retinal degeneration and optic neuropathy. In obesity syndromes, including Bardet-Biedl syndrome (BBS), Alström syndrome, and Cohen syndrome, progressive retinal degeneration is a hallmark feature. Electrophysiological techniques aid in pinpointing retinal dysfunction and tracking disease progression. Other syndromes, such as Alagille syndrome (AGS), abetalipoproteinemia (ABL), Cockayne syndrome (CS), Joubert syndrome (JS), mucopolysaccharidosis (MPS), Neuronal ceroid lipofuscinoses (NCLs), and Senior–Løken syndrome (SLS), exhibit significant ocular involvement that can be evaluated using these methods. This review underscores the role of visual electrophysiology in diagnosing and monitoring visual system abnormalities across a range of syndromes, potentially offering valuable insights for early diagnosis, monitoring of progression, and management.
Analysis of CNGC Family Members in Citrus clementina (Hort. ex Tan.) by a Genome-Wide Approach
Authors: Lv Yuanda , Liu Shumei , Ma Yanyan , Hu Lina , Yan Huaxue
Published: January 2025

The study focuses on the Cyclic nucleotide-gated ion channels (CNGCs) proteins in citrus, aiming to investigate their potential roles. A total of 33 CcCNGC proteins were identified and characterized in Citrus clementina using a genome-wide method. The study revealed that these proteins share a conserved CNGC domain structurally but exhibit significant differences in their primary sequence and motif composition. Phylogenetic analysis classified the CcCNGC proteins into 13 subgroups. The cis-elements present in all CcCNGCs promoters were identified and classified, and the number of elements was determined. The results suggested that these genes play important roles in citrus growth and development, as well as in response to biotic and abiotic stresses. Gene expression analysis further supported these findings, demonstrating that CNGC genes were responsive to various plant hormones and Phytophthora nicotianae infection, which causes citrus foot rot. Overall, the study indicated that members of the CcCNGC gene family exhibit structural and functional diversity. Further research is needed to validate the specific functions of individual family members and their roles in citrus physiology and response to stress conditions.
Infrared Laser-Assisted Extraction of Bioactive Compounds from Rosa canina L.
Authors: Alecu Andreia , Albu Camelia , Badea Georgiana-Ileana , Alionte Aurelia , Enache Alin-Alexandru , Radu Gabriel-Lucian , Litescu Simona-Carmen
Published: January 2025

The extraction of bio-compounds from medicinal plants provides opportunities for using the plant extract for health benefits. Rosa canina L. is considered a “natural superfood”, and the valorization of its active compounds requires an extraction technique that ensures a suitable extraction yield while preserving the compounds’ activity. In our study, infrared laser irradiation (IRLIR) technology was used for the first time in the bioactive compound’s extraction from Rosa canina L. Different solvents (water–ethanol, hexane–ethanol) and different extraction times were tested to obtain a high extraction yield. Chromatographic and spectrophotometry methods were used to monitor the profile of bioactive compounds and the antioxidant activity of the extracts. The results obtained for IRLIR were compared with those obtained by accelerated solvent extraction (ASE), an advanced extraction method. The IRLIR technology proved to be a more reliable analytical tool for the extraction of (+)-catechin, gallic acid, and lutein. In addition, a richer extract formula was obtained by IRLIR extraction with respect to ASE, with the IRLIR process ensuring a short extraction time, low volume of the extraction solvent, low energy consumption, and a less expensive device.
Spatial Transcriptomics in Human Cardiac Tissue
Authors: Nguyen Quynh , Tung Lin Wei , Lin Bruce , Sivakumar Raam , Sar Funda , Singhera Gurpreet , Wang Ying , Parker Jeremy , Le Bihan Stephane , Singh Amrit , M.V. Rossi Fabio , Collins Colin , Bashir Jamil , Laksman Zachary
Published: January 2025

Spatial transcriptomics has transformed our understanding of gene expression by preserving the spatial context within tissues. This review focuses on the application of spatial transcriptomics in human cardiac tissues, exploring current technologies with a focus on commercially available platforms. We also highlight key studies utilizing spatial transcriptomics to investigate cardiac development, electro-anatomy, immunology, and ischemic heart disease. These studies demonstrate how spatial transcriptomics can be used in conjunction with other omics technologies to provide a more comprehensive picture of human health and disease. Despite its transformative potential, spatial transcriptomics comes with several challenges that limit its widespread adoption and broader application. By addressing these limitations and fostering interdisciplinary collaboration, spatial transcriptomics has the potential to become an essential tool in cardiovascular research. We hope this review serves as a practical guide for researchers interested in adopting spatial transcriptomics, particularly those with limited prior experience, by providing insights into current technologies, applications, and considerations for successful implementation.
Comparison of Neural Network Structures for Identifying Shockable Rhythm During Cardiopulmonary Resuscitation
Authors: Lee Sukyo , Jung Sumin , Ahn Sejoong , Cho Hanjin , Moon Sungwoo , Park Jong-Hak
Published: January 2025

Background/Objectives: Minimizing interruptions in chest compressions is very important when resuscitating patients with cardiac arrest. Recently, research has analyzed electrocardiograms (ECGs) during chest compressions using convolutional neural networks (CNNs). This study aimed to compare the accuracy of deeper neural networks and more advanced structures. Methods: ECGs with chest compression artifacts were obtained from patients who visited the emergency department of Korea University Ansan Hospital from September 2019 to February 2024. We compared the accuracy of a deeper CNN, long short-term memory (LSTM), and a CNN with an attention mechanism and residual block against a reference model. The input of the model was 5 s ECG segments with compression artifacts, and the output was the probability that the ECG with the artifacts was a shockable rhythm. Results: A total of 1889 ECGs with compression artifacts from 172 patients were included in this study. There were 969 ECGs annotated as shockable and 920 as non-shockable. The area under the receiver operating characteristic curve (AUROC) of the reference model was 0.8672. The AUROCs of the deeper CNN for five and seven layers were 0.7374 and 0.6950, respectively. The AUROCs of LSTM and bidirectional LSTM were 0.7719 and 0.8287, respectively. The AUROC of the CNN with the attention mechanism and residual block was 0.7759. Conclusions: CNNs with deeper layers or those incorporating attention mechanisms, residual blocks, and LSTM architectures did not exhibit better accuracy. To improve the model accuracy, a larger dataset or advanced augmentation techniques may be required, rather than complicating the structure of the model.
Robot-Assisted Versus Conventional Harvesting of DIEP and Latissimus Dorsi Flaps for Breast Reconstruction in Post-Mastectomy Women: A Systematic Review and Meta-Analysis
Authors: Yusufov Stiven , Startseva Olesya , Khalfaoui Sami , Zhigailova Evgeniia , Gabriyanchik Mark , Manasherova Dina , Meskhi Kakhaber , Reshetov Igor
Published: January 2025

Background/Objectives: Robotic breast reconstruction is an innovative surgical technique that integrates robotic technology into breast reconstruction procedures, offering several advantages over conventional approaches. These benefits include enhanced visualization, increased surgical dexterity, and superior cosmetic outcomes. This study aims to comprehensively compare robotic-assisted and conventional breast reconstruction approaches in terms of complication profiles and operation-related measurements. Methods: A comprehensive search was conducted in PubMed, Embase, Scopus, Web of Science, Google Scholar, CENTRAL, and VHL from inception to October 2024 to identify relevant studies. Risk ratios for the following complications were calculated between the groups: donor site seroma, hematoma, infection, and unplanned reoperation. Mean differences were also calculated for the duration of surgery, length of postoperative hospital stays, and opioid use. Results: A meta-analysis was performed on 9 studies including a total of 1094 patients. No significant differences were found between the groups in the risk ratios for reoperation, seroma formation, delayed healing, infections, and hematomas. Similarly, there were no significant differences in postoperative opioid use. The duration of surgery was longer in the robot-assisted reconstruction group, whereas the duration of hospital stay was shorter compared to the conventional group. Meta-regression analysis for the duration of surgery model showed that none of the moderators had a statistically significant effect on this outcome. ROBINS-I assessment indicated that all the included studies had a serious risk of bias. Conclusions: Our results suggest that using a robot-assisted approach is associated with a shorter duration of hospital stay and a longer duration of surgery.
Telemedicine/Telerehabilitation to Expand Enhanced Recovery After Surgery Interventions in Minimally Invasive Mitral Valve Surgery
Authors: Malvindi Pietro Giorgio , Ceravolo Maria Gabriella , Capecci Marianna , Balestra Stefania , Cinì Emanuela , Antoniello Antonia , Pepa Lucia , Carbonetti Antonella , Ricci Maurizio , Berretta Paolo , Mazzocca Francesca , Fioretti Marco , Volpe Umberto , Munch Christopher , Di Eusanio Marco
Published: January 2025

Objectives: Having achieved a consolidated in-hospital experience with enhanced recovery after cardiac surgery, we explored the feasibility of expanding our protocol to pre-admission and post-discharge periods. Methods: A multidisciplinary team including cardiac surgeons, anaesthetists/intensivists, physiatrists, physiotherapists, perfusionists, nurses, psychiatrists, and engineers, elaborated a new therapeutic offer, based on current ERAS evidence and using telerehabilitation, to enhance preoperative communication and education and improve pre- and postoperative health and psychological state. Results: An institutional web-based platform for remote rehabilitation will host digital content that covers three main areas, including information and communication, prehabilitation and rehabilitation with the offer of respiratory and muscular exercises and aerobic activities, and psychological and patient experience evaluations. These interventions will be achieved through purposely developed video tutorials that present the hospital environments, the relevant healthcare personnel, and their role during the in-hospital patient’s journey, and illustrate tailored prehabilitation activities. A series of questionnaires will be administered to evaluate and follow the patient’s psychological state and collect patient-reported experience measures. The platform was activated in September 2024 and this service will initially involve 100 patients undergoing minimally invasive mitral valve surgery. A first review of compliance and engagement will be carried out after four months and a complete review of the results after the first year. Conclusions: ERAS is associated with improved surgical outcomes. A person-centred treatment should also address the health and psychological difficulties that patients face before hospitalisation and after discharge. Telemedicine is a valid tool to expand treatment and monitoring outside the hospital. This experience may give new insights into the feasibility and effectiveness of providing home-based remote interventions aimed at a global improvement in results throughout the overall cardiac surgery journey.
The Association Between Metabolic Syndrome and the Risk of Endometrial Cancer in Pre- and Post-Menopausal Women: A UK Biobank Study
Authors: Karkia Rebecca , Maccarthy Gideon , Payne Annette , Karteris Emmanouil , Pazoki Raha , Chatterjee Jayanta
Published: January 2025

Background: Metabolic syndrome (MetS) is a syndrome that comprises central obesity, increased serum triglyceride (TG) levels, decreased serum HDL cholesterol (HDL) levels, raised blood pressure (BP), and impaired glucose regulation, including prediabetic and diabetic glycaemic levels. Recently, the association with endometrial cancer (EC) has been described but it is unclear if the risk associated with MetS is higher than the individual effect of obesity alone. This study investigates the association between MetS components and differing MetS definitions on EC risk and compares the risk of MetS with the risk posed by obesity alone. It also analyses how MetS affects the risk of EC development in the pre- and post-menopausal subgroups. Methods: A prospective cohort study was undertaken using data from the UK biobank. Multivariable Cox proportional risk models with the time to diagnosis (years) were used to estimate the hazard ratio (HR) and 95% confidence interval (CI) of MetS and its components on the risk of EC. A subgroup analysis was also undertaken for pre- and post-menopausal participants. Kaplan–Meier (KM) was undertaken to assess the difference in the risk of EC development in differing BMI classes, and in pre- and post-menopausal subgroups. Results: A total of 177,005 females from the UK biobank were included in this study. Of those participants who developed EC (n = 1454), waist circumference > 80 cm, BMI > 30 kg/m2, hypertension > 130/80 mmHg, hyperlipidaemia and diabetes (HbA1C > 48 mmol/L were significant predictors of EC development, with waist circumference being the strongest predictor (HR = 2.21; 95% CI: 1.98–2.47, p < 0.001). Comparing the pre- and post-menopausal subgroup, hypertriglyceridaemia and diabetes were the strongest predictors of EC in the pre-menopausal subgroup (HR = 1.53; 95% CI: 1.18–1.99 and HR = 1.51; 95% CI: 1.08–2.12, p < 0.05, respectively). Raised waist circumference was not a significant independent predictor in the pre-menopausal subgroup. A KM curve analysis showed a clear distinction between those with and without MetS in the pre-menopausal group, suggesting a benefit of testing for MetS components in pre-menopausal women with obesity. Conclusions: Components of MetS, both independently and in combination, significantly increase the risk of EC. Screening those with obesity for MetS in their pre-menopausal years may help to identify those at the highest risk.