bluuebunny/crossref_metadata_2025 huggingface dataset:
Posterior reversible encephalopathy syndrome could be an underestimated variant of “reversible neurological deficits” in Systemic Lupus Erythematosus
Authors: Liu Bin , Zhang Xuan , Zhang Feng-chun , Yao Yuan , Zhou Ri-zhi , Xin Miao-Miao , Wang Li-Qin
Published: December 2012

Background

Posterior reversible encephalopathy syndrome (PRES) has been increasingly identified in patients with systemic lupus erythematosus (SLE) owing to the advance in neuroimaging techniques. Prompt diagnosis is pivotal to improve its outcome. To analyze the clinical and radiographic profile of PRES in patients with SLE and search for the appropriate treatment strategy PRES in SLE.

Methods

SLE patients who fulfilled the diagnostic criteria for PRES from August 2008 to January 2011 were evaluated at baseline, and followed to determine clinical outcomes. Data were analysis on clinical characteristics, laboratory abnormalities, treatment details, and outcomes.

Results

Ten episodes of PRES in patients with SLE were identified. All patients were female, mean age of onset was 22.93 ± 2.48 years, and SLEDAI at the onset of PRES were 25.8 ± 5.7. All cases had acute onset of headache, altered mental status, stupor, vomiting, cortical blindness and seizures. Neurological symptoms were the initial manifestation of SLE in three cases. Head magnetic resonance imaging (MRI) demonstrated posterior white matter edema involving the parietal, temporal and occipital lobes, which were more conspicuous on T2 weighted spin echo and diffusion-weighted MR imaging (DWI) than on computed tomography (CT) scan. Complete clinical and radiographic recovery was observed in 8 patients after prompt treatment with corticosteroids.

Conclusions

PRES might be due to lupus per se besides other traditional causative factors such as hypertension. PRES might be an underestimated variant of “reversible neurological deficits” in SLE. Prompt recognition and timely management is important to prevent permanent neurological deficits.
Prognostic role of CD133 expression in colorectal cancer: a meta-analysis
Authors: Wang Ke , Xu Jianjun , Zhang Junshu , Huang Jian
Published: December 2012

Background

CD133 has been identified as a putative cancer stem cell marker in colorectal cancer (CRC). However, the clinical and prognostic significance of CD133 in CRC remains controversial.

Methods

Publications were identified which assessed the clinical or prognostic significance of CD133 in CRC up to October 2012. A meta-analysis was performed to clarify the association between CD133 expression and clinical outcomes.

Results

A total of 12 studies met the inclusion criteria, and comprised 3652 cases. Analysis of these data showed that CD133 was not significantly associated with the depth of CRC invasion (odds ratio [OR] = 1.44, 95% confidence interval [CI]: 0.77–2.68, Z = 1.15, P = 0.252) or tumor differentiation (OR = 0.63, 95% CI: 0.28–1.46, Z = −1.06, P = 0.286). Also, there was no statistically significant association of CD133 with lymph node metastasis (OR = 1.16, 95% CI: 0.87–1.54, Z = 1.05, P = 0.315) or lymphatic invasion (OR = 1.08, 95% CI: 0.81–1.43, Z = 0.53, P = 0.594). However, in identified studies, overexpression of CD133 was highly correlated with reduced overall survival (relative risk [RR] = 2.14, 95% CI: 1.45–3.17, Z = 3.81, P = 0.0001).

Conclusions

CD133 may play an important role in the progression of CRC, and overexpression of CD133 is closely related with poorer patient survival. If these findings are confirmed by well-designed prospective studies, CD133 may be a useful maker for clinical applications.
Depression in older breast cancer survivors
Authors: Frazzetto Paola , Vacante Marco , Malaguarnera Michele , Vinci Ernesto , Catalano Francesca , Cataudella Emanuela , Drago Filippo , Malaguarnera Giulia , Basile Francesco , Biondi Antonio
Published: December 2012

Background

Breast cancer is the most commonly diagnosed cancer among U.S. women .The 5-year survival rate for this tumour is nowadays 85%, and the 61% of these women are still alive at 15 years. When depression symptoms are present as a consequence of breast cancer treatments, they may interfere negatively with patients’ quality of life. The aim of this study was to examine the effects of breast cancer treatment on the quality of life and the impact of depression on the health-related life.

Methods

We enrolled 173 women aged 65-75 years with early stage breast cancer diagnosed over the last 10 years, initially recruited to participate in a study examining heath-related quality of life in the first 5 years after breast cancer diagnosis. Participants were divided into four groups: 1) 46 breast cancer survivors (aged 65-70); 2) 62 women diagnosed with breast cancer (aged 65-69); 3) 32 women with recurrent breast cancer after 10 years (aged 66-75); 4) 30 women in good health status (aged 60-70). The Geriatric Depression Scale was used as a routine part of a comprehensive geriatric assessment. Collection of data for the application of instruments, such as sociodemographic variables (age, educational level, social state) and clinical date (stage and time of the disease and treatment), was carried out by trained researcher assistants.

Results

Our results demonstrated the correlation between depression and previous cancer experiences. In fact, in patients with cancer experience, the grade of depression was significantly higher compared to healthy subjects. Furthermore, we demonstrated that the patients with recurrent breast cancer were severely depressed compared to other groups.

Conclusions

A high percentage of participants were identified as having emotional and/or well being problems. Further investigations on the cause of depression problems cancer-related are needed.
Immunoliposome-PCR: a generic ultrasensitive quantitative antigen detection system
Authors: He Junkun , Evers David L , O’Leary Timothy J , Mason Jeffrey T
Published: December 2012

Background

The accurate quantification of antigens at low concentrations over a wide dynamic range is needed for identifying biomarkers associated with disease and detecting protein interactions in high-throughput microarrays used in proteomics. Here we report the development of an ultrasensitive quantitative assay format called immunoliposome polymerase chain reaction (ILPCR) that fulfills these requirements. This method uses a liposome, with reporter DNA encapsulated inside and biotin-labeled polyethylene glycol (PEG) phospholipid conjugates incorporated into the outer surface of the liposome, as a detection reagent. The antigenic target is immobilized in the well of a microplate by a capture antibody and the liposome detection reagent is then coupled to a biotin-labeled second antibody through a NeutrAvidin bridge. The liposome is ruptured to release the reporter DNA, which serves as a surrogate to quantify the protein target using real-time PCR.

Results

A liposome detection reagent was prepared, which consisted of a population of liposomes ~120 nm in diameter with each liposome possessing ~800 accessible biotin receptors and ~220 encapsulated reporters. This liposome detection reagent was used in an assay to quantify the concentration of carcinoembryonic antigen (CEA) in human serum. This ILPCR assay exhibited a linear dose–response curve from 10-10 M to 10-16 M CEA. Within this range the assay coefficient of variance was <6 % for repeatability and <2 % for reproducibility. The assay detection limit was 13 fg/mL, which is 1,500-times more sensitive than current clinical assays for CEA. An ILPCR assay to quantify HIV-1 p24 core protein in buffer was also developed.

Conclusions

The ILPCR assay has several advantages over other immuno-PCR methods. The reporter DNA and biotin-labeled PEG phospholipids spontaneously incorporate into the liposomes as they form, simplifying preparation of the detection reagent. Encapsulation of the reporter inside the liposomes allows nonspecific DNA in the assay medium to be degraded with DNase I prior to quantification of the encapsulated reporter by PCR, which reduces false-positive results and improves quantitative accuracy. The ability to encapsulate multiple reporters per liposome also helps overcome the effect of polymerase inhibitors present in biological specimens. Finally, the biotin-labeled liposome detection reagent can be coupled through a NeutrAvidin bridge to a multitude of biotin-labeled probes, making ILPCR a highly generic assay system.
Perceptual audio features for emotion detection
Authors: Sezgin Mehmet Cenk , Gunsel Bilge , Kurt Gunes Karabulut
Published: December 2012

In this article, we propose a new set of acoustic features for automatic emotion recognition from audio. The features are based on the perceptual quality metrics that are given in perceptual evaluation of audio quality known as ITU BS.1387 recommendation. Starting from the outer and middle ear models of the auditory system, we base our features on the masked perceptual loudness which defines relatively objective criteria for emotion detection. The features computed in critical bands based on the reference concept include the partial loudness of the emotional difference, emotional difference-to-perceptual mask ratio, measures of alterations of temporal envelopes, measures of harmonics of the emotional difference, the occurrence probability of emotional blocks, and perceptual bandwidth. A soft-majority voting decision rule that strengthens the conventional majority voting is proposed to assess the classifier outputs. Compared to the state-of-the-art systems including Munich Open-Source Emotion and Affect Recognition Toolkit, Hidden Markov Toolkit, and Generalized Discriminant Analysis, it is shown that the emotion recognition rates are improved between 7-16% for EMO-DB and 7-11% in VAM for "all" and "valence" tasks.
Biosorption of methyl blue onto tartaric acid modified wheat bran from aqueous solution
Authors: Yao Shuhua , Lai Hong , Shi Zhongliang
Published: December 2012

Tartaric acid modified wheat bran was utilized as adsorbent to remove methyl blue, a basic dye from aqueous solution. Batch experiments were carried out to study the effect of various experimental parameters such as initial solution pH, contact time, initial dye concentration and adsorbent dosage, on dye adsorption. The results showed that the modification of wheat bran by tartaric acid significantly improved its adsorption capacity, and made this material a suitable adsorbent to remove methyl blue. The adsorption capacity of modified wheat bran was about 1.6 times higher than that of unmodified one. The amount of methyl blue adsorbed was found to vary with initial solution pH, adsorbent dosage, contact time and initial methyl blue concentration. Kinetics study showed that the overall adsorption rate of methyl blue was illustrated by pseudo-second-order kinetic model. The applicability of the Langmuir and Freundlich models for the data was tested. Both models adequately described the experimental data of the biosorption of methyl blue. The maximum adsorption capacity for methyl blue calculated from Langmuir model was 25.18 mg/g. The study has shown the effectiveness of modified wheat bran in the removal of methyl blue, and that it can be considered as an attractive alternative to the more expensive technologies used in wastewater treatment.
Effects of phlebotomy-induced reduction of body iron stores on metabolic syndrome: results from a randomized clinical trial
Authors: Houschyar Khosrow S , Lüdtke Rainer , Dobos Gustav J , Kalus Ulrich , Broecker-Preuss Martina , Rampp Thomas , Brinkhaus Benno , Michalsen Andreas
Published: December 2012

Background

Metabolic syndrome (METS) is an increasingly prevalent but poorly understood clinical condition characterized by insulin resistance, glucose intolerance, dyslipidemia, hypertension, and obesity. Increased oxidative stress catalyzed by accumulation of iron in excess of physiologic requirements has been implicated in the pathogenesis of METS, but the relationships between cause and effect remain uncertain. We tested the hypothesis that phlebotomy-induced reduction of body iron stores would alter the clinical presentation of METS, using a randomized trial.

Methods

In a randomized, controlled, single-blind clinical trial, 64 patients with METS were randomly assigned to iron reduction by phlebotomy (n = 33) or to a control group (n = 31), which was offered phlebotomy at the end of the study (waiting-list design). The iron-reduction patients had 300 ml of blood removed at entry and between 250 and 500 ml removed after 4 weeks, depending on ferritin levels at study entry. Primary outcomes were change in systolic blood pressure (SBP) and insulin sensitivity as measured by Homeostatic Model Assessment (HOMA) index after 6 weeks. Secondary outcomes included HbA1c, plasma glucose, blood lipids, and heart rate (HR).

Results

SBP decreased from 148.5 ± 12.3 mmHg to 130.5 ± 11.8 mmHg in the phlebotomy group, and from 144.7 ± 14.4 mmHg to 143.8 ± 11.9 mmHg in the control group (difference -16.6 mmHg; 95% CI -20.7 to -12.5;P< 0.001). No significant effect on HOMA index was seen. With regard to secondary outcomes, blood glucose, HbA1c, low-density lipoprotein/high-density lipoprotein ratio, and HR were significantly decreased by phlebotomy. Changes in BP and HOMA index correlated with ferritin reduction.

Conclusions

In patients with METS, phlebotomy, with consecutive reduction of body iron stores, lowered BP and resulted in improvements in markers of cardiovascular risk and glycemic control. Blood donation may have beneficial effects for blood donors with METS.

Trial registration

ClinicalTrials.gov:NCT01328210

Please see related article:http://www.biomedcentral.com/1741-7015/10/53
Leptin differentially regulate STAT3 activation in ob/ob mouse adipose mesenchymal stem cells
Authors: Zhou Zhou , Neupane Manish , Zhou Hui Ren , Wu Dayong , Chang Chia-Cheng , Moustaid-Moussa Naima , Claycombe Kate J
Published: December 2012

Background

Leptin-deficient ob/ob mice exhibit adipocyte hypertrophy and hyperplasia as well as elevated adipose tissue and systemic inflammation. Multipotent stem cells isolated from adult adipose tissue can differentiate into adipocytes ex vivo and thereby contribute toward increased adipocyte cell numbers, obesity, and inflamm ation. Currently, information is lacking regarding regulation of adipose stem cell numbers as well as leptin-induced inflammation and its signaling pathway in ob/ob mice.

Methods

Using leptin deficient ob/ob mice, we investigated whether leptin injection into ob/ob mice increases adipose stem cell numbers and adipose tissue inflammatory marker MCP-1 mRNA and secretion levels. We also determined leptin mediated signaling pathways in the adipose stem cells.

Results

We report here that adipose stem cell number is significantly increased following leptin injection in ob/ob mice and with treatment of isolated stem cells with leptin in vitro. Leptin also up-regulated MCP-1 secretion in a dose- and time-dependent manner. We further showed that increased MCP-1 mRNA levels were due to increased phosphorylation of Signal Transducer and Activator of Transcription 3 (STAT3) Ser727 but not STAT3 Tyr705 phosphorylation, suggesting differential regulation of MCP-1 gene expression under basal and leptin-stimulated conditions in adipose stem cells.

Conclusions

Taken together, these studies demonstrate that leptin increases adipose stem cell number and differentially activates STAT3 protein resulting in up-regulation of MCP-1 gene expression. Further studies of mechanisms mediating adipose stem cell hyperplasia and leptin signaling in obesity are warranted and may help identify novel anti-obesity target strategies.
The superhealing MRL background improves muscular dystrophy
Authors: Heydemann Ahlke , Swaggart Kayleigh A , Kim Gene H , Holley-Cuthrell Jenan , Hadhazy Michele , McNally Elizabeth M
Published: December 2012

Background

Mice from the MRL or “superhealing” strain have enhanced repair after acute injury to the skin, cornea, and heart. We now tested an admixture of the MRL genome and found that it altered the course of muscle pathology and cardiac function in a chronic disease model of skeletal and cardiac muscle. Mice lacking γ-sarcoglycan (Sgcg), a dystrophin-associated protein, develop muscular dystrophy and cardiomyopathy similar to their human counterparts with limb girdle muscular dystrophy. With disruption of the dystrophin complex, the muscle plasma membrane becomes leaky and muscles develop increased fibrosis.

Methods

MRL/MpJ mice were bred with Sgcg mice, and cardiac function was measured. Muscles were assessed for fibrosis and membrane leak using measurements of hydroxyproline and Evans blue dye. Quantitative trait locus mapping was conducted using single nucleotide polymorphisms distinct between the two parental strains.

Results

Introduction of the MRL genome reduced fibrosis but did not alter membrane leak in skeletal muscle of the Sgcg model. The MRL genome was also associated with improved cardiac function with reversal of depressed fractional shortening and the left ventricular ejection fraction. We conducted a genome-wide analysis of genetic modifiers and found that a region on chromosome 2 was associated with cardiac, diaphragm muscle and abdominal muscle fibrosis.

Conclusions

These data are consistent with a model where the MRL genome acts in a dominant manner to suppress fibrosis in this chronic disease setting of heart and muscle disease.
Parasite-augmented mate choice and reduction in innate fear in rats infected byToxoplasma gondii
Authors: Vyas Ajai
Published: December 2012

Typically, female rats demonstrate clear mate choice. Mate preference is driven by the evolutionary need to choose males with heritable parasite resistance and to prevent the transmission of contagious diseases during mating. Thus, females detect and avoid parasitized males. Over evolutionary time scales, parasite-free males plausibly evolve to advertise their status. This arrangement between males and females is obviously detrimental to parasites, especially for sexually transmitted parasites. Yet Toxoplasma gondii, a sexually transmitted parasite, gets around this obstacle by manipulating mate choice of uninfected females. Males infected with this parasite become more attractive to uninfected females. The ability of T. gondii to not only advantageously alter the behavior and physiology of its host but also secondarily alter the behavior of uninfected females presents a striking example of the ‘extended phenotype’ of parasites. Toxoplasma gondii also abolishes the innate fear response of rats to cat odor; this likely increases parasite transmission through the trophic route. It is plausible that these two manipulations are not two distinct phenotypes, but are rather part of a single pattern built around testosterone-mediated interplay between mate choice, parasitism and predation.
CINtec PLUS Immunocytochemistry as a Tool for the Cytologic Diagnosis of Glandular Lesions of the Cervix Uteri
Authors: Ravarino Alberto , Nemolato Sonia , Macciocu Elena , Fraschini Matteo , Senes Giancarlo , Faa Gavino , Negri Giovanni
Published: December 2012

Cytologic findings of glandular lesions of the cervix uteri are often difficult to evaluate. We studied the usefulness of CINtec PLUS p16/Ki-67 double stain (mtm laboratories, Heidelberg, Germany) for the diagnosis of glandular lesions. The study included 47 abnormal results on liquid-based cytologic tests with a subsequent histologic diagnosis of adenocarcinoma in situ or with early invasion, and 16 samples with negative results on follow-up. All samples were stained with CINtec PLUS p16/Ki-67 double stain. Of the neoplastic samples, 7 were excluded because of insufficient residual cellularity or loss of neoplastic cells. Of the samples that were adequate, 92.5% were stained with CINtec PLUS, whereas 7.5% were judged inconclusive. All inconclusive cases were at least 3 years old. Of the 16 negative samples, 15 (93.8%) stained negative and only 1 (6.2%) showed several positive clusters of cells. Our study shows that CINtec PLUS is a robust and useful tool for the diagnosis of glandular lesions of the cervix uteri.
Avoiding Serotonin Syndrome: The Nature of the Interaction Between Tramadol and Selective Serotonin Reuptake Inhibitors
Authors: Nelson Elana M , Philbrick Ann M
Published: December 2012

Objective:

To investigate the nature of the interaction between selective serotonin reuptake inhibitors (SSRIs) and tramadol to mitigate or avoid serotonin syndrome.

Data Sources:

PubMed, Ovid MEDLINE, and International Pharmaceutical Abstracts from January 1990 to August 2012 were searched. Key words used were tramadol, antidepressive agents, antidepressants, drug interactions, selective serotonin uptake inhibitors, and serotonin syndrome.

Study Selection and Data Extraction:

Only English-language studies were included. No randomized controlled trials were identified. Review articles, case reports, and 1 case series that identified the scope of interaction between tramadol and SSRIs were evaluated. Review articles evaluating the role of pharmacogenetics in the use of tramadol, SSRIs, and serotonin syndrome were also reviewed.

Data Synthesis:

Published documentation describing the interaction between tramadol and SSRIs and its relevance to serotonin syndrome is limited to a few case reports and 1 case series. While both tramadol and SSRIs increase the amount of serotonin in the brain, the interaction is much more complicated. Tramadol is metabolized through CYP2D6 enzymes and all SSRIs are inhibitors of these enzymes. Inhibitors of CYP2D6 can increase the concentration of tramadol in the blood and thus increase its effects on serotonin in the brain, contributing to the development of serotonin syndrome. CYP2D6 poor metabolizers are at a greater risk of serotonin syndrome and an inadequate analgesic effect.

Conclusions:

Coadministration of tramadol and SSRIs has caused serotonin syndrome. An attempt should be made to identify individuals who are poor metabolizers of CYP2D6 and avoid this combination in those patients. When SSRIs and tramadol must be used in combination, it is critical that patients be aware of the signs and symptoms of serotonin syndrome, should they occur.
Mineralogical Evolution of a Claystone After Reaction With Iron Under Thermal Gradient
Authors: Jodin-Caumon Marie-Camille , Mosser-Ruck Régine , Randi Aurélien , Pierron Olivier , Cathelineau Michel , Michau Nicolas
Published: December 2012

The design of the repository for high-level nuclear waste (HLW) in France consists of a multiple-barrier system including steel canisters in a clay host rock. The system will undergo temperature variations in time and space, the heat source being the HLW within the canisters. The effect of a thermal gradient in space on the Fe-claystone interaction was investigated here by applying a thermal gradient (150–300°C and 80–150°C) to a mix of claystone, Fe, and an aqueous chloride solution over periods of 3 and 6 months. Following the reaction, the starting clay minerals (mostly illite and mixed-layer illite-smectite) evolved toward chlorite, Fe-serpentine, Fe-saponite, mixed-layer chlorite-smectite, or mixed-layer serpentine-smectite as a function of temperature. Iron corrosion made the medium basic and reductive. Magnesium enrichment of clay minerals was observed in the hottest part of the experiment due to Mg migration under the thermal gradient. Reaction progress was enhanced at the lowest temperatures, compared to batch experiments.
Rietveld Refinement of Disordered Illite-Smectite Mixed-Layer Structures by a Recursive Algorithm. I: One-Dimensional Patterns
Authors: Ufer Kristian , Kleeberg Reinhard , Bergmann Jörg , Dohrmann Reiner
Published: December 2012

X-ray diffraction patterns of oriented mounts of clay minerals are often used in clay mineralogy for qualitative and quantitative purposes. Fequently occurring stacking defects, in particular, can be characterized by this technique. Modeling of these diffraction profiles has become an important tool in obtaining structural information about the nature of stacking order. Manual matching of calculated and observed patterns is time consuming and user dependent. Automatic refinement procedures are, therefore, desirable. An improved approach for the treatment of disordered layer structures within a Rietveld refinement is presented here. The recursive calculation of structure factors, similar to that of the simulation program DIFFaX, was introduced in the Rietveld code BGMN. Complete implementation is formulated within the interpreter language of the Rietveld code and is transparent as well as flexible. Such a method has opened the application of Rietveld refinement to patterns of oriented mounts where only basal reflections of stacking disordered structures were recorded. The DIFFaX code was used to simulate basal reflections of illite-smectite mixed layers (I-S) with different ratios of illitic and smectitic layers and with different degrees of long-range ordering (Reichweite). Rietveld refinements with these simulated patterns were used to evaluate the application of this new approach. Several I-S with different degrees of ordering were also chosen as tests for the refinement of basal reflections. The samples were prepared as standard airdried and ethylene glycol-solvated, oriented specimens. Realistic structural parameters were obtained for the composition and ordering of the I-S.
Multicenter Evaluation of [−2]Proprostate-Specific Antigen and the Prostate Health Index for Detecting Prostate Cancer
Authors: Stephan Carsten , Vincendeau Sébastien , Houlgatte Alain , Cammann Henning , Jung Klaus , Semjonow Axel
Published: December 2012

BACKGROUND

Total prostate-specific antigen (tPSA) is flawed for prostate cancer (PCa) detection. [−2]proprostate-specific antigen (p2PSA), a molecular isoform of free PSA (fPSA), shows higher specificity compared with tPSA or percentage of free PSA (%fPSA). The prostate health index (Phi), a measure based on p2PSA and calculated as p2PSA/fPSA × √tPSA, was evaluated in a multicenter study for detecting PCa.

METHODS

A total of 1362 patients from 4 different study sites who had tPSA values of 1.6–8.0 μg/L (668 patients with PCa, 694 without PCa) underwent ≥10 core biopsies. Serum concentrations of tPSA, fPSA (both calibrated against a WHO reference material), and p2PSA were measured on Access2 or DxI800 analyzers (Beckman Coulter).

RESULTS

The percentage ratio of p2PSA to fPSA (%p2PSA) and Phi were significantly higher in all PCa subcohorts (positive initial or repeat biopsy result or negative digital rectal examination) (P < 0.0001) compared with patients without PCa. Phi had the largest area under the ROC curve (AUC) (AUC = 0.74) and provided significantly better clinical performance for predicting PCa compared with %p2PSA (AUC = 0.72, P = 0.018), p2PSA (AUC = 0.63, P < 0.0001), %fPSA (AUC = 0.61) or tPSA (AUC = 0.56). Significantly higher median values of Phi were observed for patients with a Gleason score ≥7 (Phi = 60) compared with a Gleason score <7 (Phi = 53; P = 0.0018). The proportion of aggressive PCa (Gleason score ≥7) increased with the Phi score.

CONCLUSIONS

The results of this multicenter study show that Phi, compared with tPSA or %fPSA, demonstrated superior clinical performance in detecting PCa at tPSA 1.6–8.0 μg/L (i.e., approximately 2–10 μg/L in traditional calibration) and is better able to detect aggressive PCa.
Revisiting the Cultural Policy Moment: Queensland Cultural Policy from Goss to Bligh
Authors: Glover Stuart
Published: December 2012

An account of cultural policy-making in Queensland since the election of the Goss Labor government in 1989 requires revisiting the rise and fall of what Stevenson (2000) has called the ‘cultural policy moment’ in Australia.

This period, from the early 1990s to the early 2000s, was characterised by political and scholarly interest in the civic and symbolic utility of culture, and in the outcomes achieved through its management. The cultural policy moment was produced simultaneously within government, the cultural sector and the academy. Within government, it was characterised by a new and highly visible interest in managing culture and (through it) the citizenry (O'Regan 2002). Within the academy, the cultural policy project was raised by Tim Rowse in Arguing the Arts (1985) and developed by the Institute for Cultural Policy Studies at Griffith University through the work of Ian Hunter, Tony Bennett, Toby Miller, Colin Mercer, Jenny Craik, Tom O'Regan and Gay Hawkins in the late 1980s and early 1990s. Stuart Cunningham's Framing Culture (1992) focused existing debate within Australian cultural studies over the place of policy-based approaches within the discipline.
Mrp14 Deficiency Ameliorates Amyloid β Burden by Increasing Microglial Phagocytosis and Modulation of Amyloid Precursor Protein Processing
Authors: Kummer Markus P. , Vogl Thomas , Axt Daisy , Griep Angelika , Vieira-Saecker Ana , Jessen Frank , Gelpi Ellen , Roth Johannes , Heneka Michael T.
Published: December 2012

Neuroinflammation plays a fundamental role in the pathogenesis of Alzheimer's disease (AD), resulting in the extensive activation of microglial and astroglial cells. Here we describe the role of myeloid-related protein Mrp14, a recently described amplifier of inflammation, in Alzheimer's disease and in the related amyloid precursor protein/presenilin1 (APP/PS1) mouse model. Detection of Mrp14 in control, mildly cognitive impaired, and AD patients revealed a strong induction of Mrp14 in protein extracts as well as in the cerebrospinal fluid, but not in blood plasma. In APP/PS1 mice, Mrp14 and its heterodimeric partner Mrp8 was found to be upregulated in microglial cells surrounding amyloid plaques. Functionally, loss of Mrp14 led to increased phagocytosis of fibrillar amyloid β (Aβ) in microglia cellsin vitroandin vivo. Generating APP/PS1-transgenic mice deficient for Mrp14, we observed a decrease of key cytokines involved in APP processing, a reduction of BACE1 expression and activity, and consequently overall Aβ deposition. We therefore conclude that Mrp14 promotes APP processing and Aβ accumulation under neuroinflammatory conditions.
Stress Produces Aversion and Potentiates Cocaine Reward by Releasing Endogenous Dynorphins in the Ventral Striatum to Locally Stimulate Serotonin Reuptake
Authors: Schindler Abigail G. , Messinger Daniel I. , Smith Jeffrey S. , Shankar Haripriya , Gustin Richard M. , Schattauer Selena S. , Lemos Julia C. , Chavkin Nicholas W. , Hagan Catherine E. , Neumaier John F. , Chavkin Charles
Published: December 2012

Activation of the dynorphin/κ-opioid receptor (KOR) system by repeated stress exposure or agonist treatment produces place aversion, social avoidance, and reinstatement of extinguished cocaine place preference behaviors by stimulation of p38α MAPK, which subsequently causes the translocation of the serotonin transporter (SERT, SLC6A4) to the synaptic terminals of serotonergic neurons. In the present study we extend those findings by showing that stress-induced potentiation of cocaine conditioned place preference occurred by a similar mechanism. In addition, SERT knock-out mice did not show KOR-mediated aversion, and selective reexpression of SERT by lentiviral injection into the dorsal raphe restored the prodepressive effects of KOR activation. Kinetic analysis of several neurotransporters demonstrated that repeated swim stress exposure selectively increased theVmaxbut notKmof SERT without affecting dopamine transport or the high-capacity, low-affinity transporters. Although the serotonergic neurons in the dorsal raphe project throughout the forebrain, a significant stress-induced increase in cell-surface SERT expression was only evident in the ventral striatum, and not in the dorsal striatum, hippocampus, prefrontal cortex, amygdala, or dorsal raphe. Stereotaxic microinjections of the long-lasting KOR antagonist norbinaltorphimine demonstrated that local KOR activation in the nucleus accumbens, but not dorsal raphe, mediated this stress-induced increase in ventral striatal surface SERT expression. Together, these results support the hypothesis that stress-induced activation of the dynorphin/KOR system produces a transient increase in serotonin transport locally in the ventral striatum that may underlie some of the adverse consequences of stress exposure, including the potentiation of the rewarding effects of cocaine.
Satb1 Is an Activity-Modulated Transcription Factor Required for the Terminal Differentiation and Connectivity of Medial Ganglionic Eminence-Derived Cortical Interneurons
Authors: Close Jennie , Xu Han , De Marco García Natalia , Batista-Brito Renata , Rossignol Elsa , Rudy Bernardo , Fishell Gord
Published: December 2012

Although previous work identified transcription factors crucial for the specification and migration of parvalbumin (PV)-expressing and somatostatin (SST)-expressing interneurons, the intrinsic factors required for the terminal differentiation, connectivity, and survival of these cell types remain uncharacterized. Here we demonstrate that, within subpopulations of cortical interneurons,Satb1(special AT-rich binding protein) promotes terminal differentiation, connectivity, and survival in interneurons that express PV and SST. We find that conditional removal ofSatb1in mouse interneurons results in the loss of a majority of SST-expressing cells across all cortical layers, as well as some PV-expressing cells in layers IV and VI, by postnatal day 21. SST-expressing cells initially migrate to the cortex inSatb1mutant mice, but receive reduced levels of afferent input and begin to die during the first postnatal week. Electrophysiological characterization indicates that loss ofSatb1function in interneurons results in a loss of functional inhibition of excitatory principal cells. These data suggest that Satb1 is required for medial ganglionic eminence-derived interneuron differentiation, connectivity, and survival.
Bone mineral density, bone markers, and fractures in adult males with congenital adrenal hyperplasia
Authors: Falhammar Henrik , Filipsson Nyström Helena , Wedell Anna , Brismar Kerstin , Thorén Marja
Published: December 2012

Objective

The aim of this study was to determine bone mineral density (BMD), markers of bone metabolism, fractures, and steroids reflecting hormonal control in adult males with congenital adrenal hyperplasia (CAH).

Subjects, methods, and design

We compared CAH males with 21-hydroxylase deficiency (n=30), 19–67 years old, with age- and sex-matched controls (n=32). Subgroups ofCYP21A2genotypes, age, glucocorticoid preparation, poor control vs overtreatment, and early vs late (>36 months) diagnosis were studied. BMD measured by dual energy X-ray absorptiometry and markers of bone metabolism and androgens/17-hydroxyprogesterone levels were investigated.

Results

All, including older (>30 years), CAH patients had lower BMD in all measured sites compared with control subjects. The null group demonstrated lower BMD in more locations than the other groups. Osteoporosis/osteopenia was present in 81% of CAH patients compared with 32% in controls (≥30 years). Fracture frequency was similar, osteocalcin was lower, and fewer patients than controls had vitamin D insufficiency. IGF1 was elevated in the milder genotypes. In patients, total body BMD was positively correlated to weight, BMI, total lean body mass, and triglycerides, and negatively to prolactin. Patients on prednisolone had lower BMD and osteocalcin levels than those on hydrocortisone/cortisone acetate. Patients with poor control had higher femoral neck BMD. There were no differences in BMD between patients with an early vs late diagnosis.

Conclusions

CAH males have low BMD and bone formation markers. BMD should be monitored, adequate prophylaxis and treatment established, and glucocorticoid doses optimized to minimize the risk of future fractures.
Elementos de integralidade nas práticas profissionais de saúde a mulheres rurais vítimas de violência
Authors: Costa Marta Cocco da , Lopes Marta Julia Marques
Published: December 2012

O presente artigo trata de estudo qualitativo que buscou conhecer e analisar práticas de cuidado de profissionais de saúde a mulheres rurais vítimas de violência, na perspectiva da atenção integral, em municípios da Metade Sul, RS. Participaram da pesquisa, profissionais e trabalhadores dos serviços de saúde, que atuam em áreas rurais. As informações foram geradas por meio de entrevista e analisadas pela modalidade temática. Apontam-se como elementos de cuidados às usuárias rurais em situações de violência não só os dispositivos relacionais - acolhimento, vínculo e diálogo - como também a construção de ações coletivas por meio de atividades grupais, reconhecidas como potencializadoras da promoção da saúde e do empoderamento individual e coletivo na dimensão dos eventos violentos. Constatou-se que nas práticas de cuidado dos profissionais há um direcionamento para a inclusão das usuárias rurais como protagonista do cuidado, estabelecendo uma relação entre trabalhador-usuária para a produção da integralidade.
Eficácia da auriculoterapia na redução de ansiedade em estudantes de enfermagem
Authors: Prado Juliana Miyuki do , Kurebayashi Leonice Fumiko Sato , Silva Maria Júlia Paes da
Published: December 2012

Este Ensaio Clínico Randomizado simples-cego verificou níveis de ansiedade dos estudantes de Enfermagem de nível médio da Escola de Enfermagem São Joaquim, do Hospital Beneficência Portuguesa, e a eficácia da auriculoterapia na redução desses níveis. Foi aplicado o Inventário de Ansiedade Traço-Estado no início, após 8 e 12 sessões e no follow-up (quinze dias). A amostra foi composta por 71 indivíduos divididos em três grupos: Controle sem intervenção (25), Auriculoterapia (24), e Placebo (22). Resultados: Na análise de variância (ANOVA) houve diferença estatisticamente significativa pelo Post Hoc entre os grupos controle/auriculoterapia na segunda (p=0.000), terceira (p=0.012) e quarta avaliações (p=0.005); e entre grupos placebo/controle, somente na 2ª avaliação (p=0.003). A auriculoterapia com os pontos Shenmen e Tronco Cerebral foi mais eficaz para a diminuição dos níveis de ansiedade em estudantes de Enfermagem (20,97%), em comparação com os pontos sham (13,74%), porém, estudos com amostragem mais representativa se fazem necessários.
Parâmetros hematológicos e alterações histopatológicas em bijupirá (Rachycentron canadum Linnaeus, 1766) com amyloodiniose
Authors: Guerra-Santos Bartira , Albinati Ricardo C.B. , Moreira Eduardo Luiz T. , Lima Fernanda W.M. , Azevedo Tatiana M.P. de , Costa Denise S.P. , Medeiros Silene D.C. de , Lira Alessandra D.
Published: December 2012

O objetivo do trabalho foi descrever os parâmetros hematológicos e as alterações histopatológicas em bijupirás infectados por Amyloodinium ocellatum. Um grupo de 27 peixes foi anestesiado para coleta de amostras de sangue e eutanasiados para coleta de muco e fragmentos de tecido cutâneo e branquial. Foram avaliadas a prevalência e a intensidade parasitária da infecção, assim como os valores de parâmetros hematológicos e alterações histopatológicas. A prevalência parasitária nas brânquias foi de 100% e no muco foi de 80,8% e as intensidades parasitárias médias foram de 683,5 nas brânquias, e 67,1 no muco cutâneo. Os valores médios dos parâmetros hematológicos foram: eritrócitos 4,3x10(6)µL; VG 26%; VGM 64,2fL; proteína plasmática 5,8mg/dL; trombócitos 5,2 x10³/µL e leucócitos 3,6 x10³/µL. Além disso, foram verificadas hiperplasia do epitélio respiratório acompanhada de fusão lamelar, descolamento do epitélio, dilatação do seio venoso, formação de aneurisma, ruptura do epitélio lamelar, hemorragia, necrose, reação inflamatória linfocítica. O parasito foi observado nas lamelas branquiais, o VMA variou do grau discreto ao severo e o IAH foi de 76,8. A pesquisa assume importância por se tratar dos primeiros estudos em Rachycentron canadum, um peixe que se destaca com potencial ao cultivo.
Desenvolvimento corporal e testicular em machos de codornas de corte e de postura de 25 a 360 dias
Authors: Santos Tatiana C. , Murakami Alice E. , Oliveira Carlos A.L. , Costa Paula D.
Published: December 2012

Codornas do tipo carne e tipo ovos foram analisadas para determinar o desenvolvimento reprodutivo, a puberdade e o início da senilidade. Para tal, 288 codornas (144 codornas de corte e 144 de postura) foram acompanhadas desde a eclosão até os 360 dias de idade. As aves foram distribuídas por idade em 18 grupos, sendo 8 codornas/grupo/tipo de codorna. Após 35 dias as codornas foram mantidas em condições de fotoperíodo de dias longos (17luz: 7escuro). O peso vivo e os valores morfométricos e histológicos testiculares foram determinados em cada período. Os dados obtidos foram analisados para determinar a curva de crescimento e o comportamento dos parâmetros analisados. O modelo que mais se adequou aos dados foi o modelo não linear de Gompertz (Y=A exp [-B e (-kt)]). O peso vivo e as características testiculares macro e microscópicas apresentaram comportamento alométrico entre si, sendo que, aproximadamente aos 60 dias os machos apresentaram-se sexualmente desenvolvidos, e estabilizaram o peso corporal por volta dos 100 dias. O testículo direito é mais cranial que o esquerdo e diferem em relação a comprimento e largura, porém não foi observada diferenças (P>0,05) para peso testicular. As codornas de corte apresentaram peso corporal e peso testicular maiores que as codornas de postura, porém as codornas de postura apresentaram peso relativo testicular maior. Durante todo o período analisado os machos puderam ser considerados sexualmente aptos. Os reprodutores apresentaram características sexuais ativas até os 360 dias de idade, representadas pelo tamanho testicular e pela atividade celular nos túbulos seminíferos.
Efeito alelopático de folhas de bamburral [Hyptis suaveolens (L.) Poit.] sobre a germinação de sementes de sorgo (Sorghum vulgare Pers.), rabanete (Raphanus sativus L.) e alface (Lactuca sativa L.)
Authors: Rodrigues A.C. , Artioli F.A. , Polo M. , Barbosa L.C.A. , Beijo L.A.
Published: December 2012

O objetivo do presente trabalho foi à verificação do efeito alelopático de Hyptis. suaveolens na germinação de sorgo, alface e rabanete, bem como, a comprovação da existência de compostos com potencial alelopático. Sementes de sorgo, alface e rabanete foram semeadas em substrato constituído de areia, terra e adubo orgânico contendo folhas de H. suaveolens. As análises da germinação foram feitas considerando a protrusão da radícula para o término do evento germinativo. Foi calculado o IVG (índice de velocidade de germinação) e %G (porcentagem de germinação). Os resultados mostraram que sorgo e a alface foram mais susceptíveis ao potencial alelopático de H. suaveolens, sendo que para o rabanete foi observado um efeito benéfico. Entre os tratamentos, o substrato esterilizado e não esterilizado mostraram diferenças entre si. A análise cromatográfica do óleo essencial presente nas folhas de H. suaveolens revelou a presença de compostos com potencial alelopático. Portanto, H. suaveolens, pode apresentar efeito alelopático positivo no IVG de sementes de rabanete e a presença de microorganismos pode ser necessária para que esse efeito alelopático aconteça.
Questionário nutricional simplificado de apetite (QNSA) para uso em programas de reabilitação cardiopulmonar e metabólica
Authors: Sties Sabrina Weiss , Gonzáles Ana Inês , Viana Maick da Silveira , Brandt Ricardo , Bertin Renata Labronici , Goldfeder Ricardo , Ulbrich Anderson Zampier , Andrade Alexandro , Carvalho Tales de
Published: December 2012

O presente estudo teve por objetivo traduzir e validar para a língua portuguesa o Questionário Nutricional Simplificado de Apetite (QNSA), verificando sua clareza e validade para participantes de programa de Reabilitação Cardiopulmonar e Metabólica (RCPM). Cento e quarenta e seis indivíduos foram entrevistados, sendo 79 homens, com média de idade de 63 anos (± 10) e 67 mulheres, com média de 66 anos (± 11). A análise descritiva foi apresentada em média, desvio padrão e frequência. A consistência interna da escala foi avaliada por meio do coeficiente alfa de Cronbach e a análise fatorial pelo método de extração de análise dos componentes principais utilizando os critérios de Kaiser (autovalores > 1). Todas as questões do instrumento apresentaram resultados positivos em relação à clareza. Os itens do questionário apresentaram carga fatorial superior a 0,40, com variação de 0,40 a 0,81. Com exceção da questão relacionada com a quantidade de refeições diárias, todas as demais apresentaram alta correlação com o escore total da versão brasileira do instrumento. No que se refere à análise de consistência interna, obteve-se resultado de 0,61. A versão brasileira do QNSA demonstrou ser válida para aplicação em participantes de programa de RCPM, constituindo-se em uma importante ferramenta na avaliação do apetite nessa população.
EMERGENT INTRAVERBAL RESPONSES VIA TACT AND MATCH‐TO‐SAMPLE INSTRUCTION
Authors: Grannan Leigh , Rehfeldt Ruth Anne
Published: December 2012

The present investigation evaluated the effectiveness of category tact and match‐to‐sample instruction in facilitating the emergence of intraverbal responses (i.e., naming several items belonging to a specific category) for 2 children with autism. Results demonstrated the emergence of untaught responses, suggesting an effective instructional protocol for establishing intraverbal responses without direct instruction.
Combating Solids Precipitation and Depostition in ADHI Gas-Condensate Process Plant
Authors: Ahmed Shujjat , Qadeer Suhail , Bouamra Reda , Chaker Ahmed Abu , Jamaiuddin Abul
Published: December 2012

Adhi gas—condensate field is located near Islamabad, Pakistan. Pakistan Petroleum Limited started fluid processing and recovery of Liquefied Petroleum Gas and Condensate around in 1990. The liquid stream was processed with no solids deposition in the past. Recently, the liquid processing circuit of the plant has experienced an increasing amount of black solid deposition, which is trapped into the liquid filters located in the plant.

To identify the root causes of the problem of these solids depositional systematic approach was applied including taking various solid, liquid and gas samples from the plant inlet and various locations inside the processing plant and analyzing them for diagnostics.

Based on the outcome of the root-cause analysis, a chemical mitigation strategy has been developed, tested and implemented, resulting in significant reduction in problems related with solid depositions in processing plant.