bluuebunny/crossref_metadata_2025 huggingface dataset:
Building 1D and 3D Mechanical Earth Models for Underground Gas Storage—A Case Study from the Molasse Basin, Southern Germany
Authors: Zain-Ul-Abedin Muhammad , Henk Andreas
Published: November 2020

Hydromechanical models of gas storage in porous media provide valuable information for various applications ranging from the prediction of ground surface displacements to the determination of maximum reservoir pressure and storage capacity to maintain fault stability and caprock integrity. A workflow to set up such models is presented and applied to a former gas field in southern Germany for which transformation to a gas storage site is considered. The workflow comprises 1D mechanical earth modeling (1D MEM) to calculate elastic properties as well as a first estimate for the vertical and horizontal stresses at well locations by using log data. This information is then used to populate a 3D finite element model (3D MEM) which has been built from seismic data and comprises not only the reservoir but the entire overburden up to the earth’s surface as well as part of the underburden. The size of this model is 30 × 24 × 5 km3. The pore pressure field has been derived from dynamic fluid flow simulation through history matching for the production and subsequent shut-in phase. The validated model is ready to be used for analyzing new wells for future field development and testing arbitrary injection-production schedules, among others.
Estimation of the Heat Loss Coefficient of Two Occupied Residential Buildings through an Average Method
Authors: Uriarte Irati , Erkoreka Aitor , Eguia Pablo , Granada Enrique , Martin-Escudero Koldo
Published: November 2020

The existing performance gap between the design and the real energy consumption of a building could have three main origins: the occupants’ behaviour, the performance of the energy systems and the performance of the building envelope. Through the estimation of the in-use Heat Loss Coefficient (HLC), it is possible to characterise the building’s envelope energy performance under occupied conditions. In this research, the estimation of the HLC of two individual residential buildings located in Gainsborough and Loughborough (UK) was carried out using an average method. This average method was developed and successfully tested in previous research for an occupied four-story office building with very different characteristics to individual residential buildings. Furthermore, one of the analysed residential buildings is a new, well-insulated building, while the other represents the old, poorly insulated semidetached residential building typology. Thus, the monitored data provided were filtered in order to apply the abovementioned average method. Even without fulfilling all the average method requirements for these two residential buildings, the method provides reliable HLC values for both residential buildings. For the house in Gainsborough, the best estimated HLC value was 60.2 W/K, while the best approach for Loughborough was 366.6 W/K. Thus, despite the uncertainty sources found during the analysis, the method seems promising for its application to residential buildings.
An Algorithm for Genetic Analysis of Full-Sib Datasets with Mixed-Model Software Lacking a Numerator Relationship Matrix Function, and a Comparison with Results from a Dedicated Genetic Software Package
Authors: Hodge Gary R. , Acosta Juan Jose
Published: November 2020

Research Highlights: An algorithm is presented that allows for the analysis of full-sib genetic datasets using generalized mixed-model software programs. The algorithm produces variance component estimates, genetic parameter estimates, and Best Linear Unbiased Prediction (BLUP) solutions for genetic values that are, for all practical purposes, identical to those produced by dedicated genetic software packages. Background and Objectives: The objective of this manuscript is to demonstrate an approach with a simulated full-sib dataset representing a typical forest tree breeding population (40 parents, 80 full-sib crosses, 4 tests, and 6000 trees) using two widely available mixed-model packages. Materials and Methods: The algorithm involves artificially doubling the dataset, so that each observation is in the dataset twice, once with the original female and male parent identification, and once with the female and male parent identities switched. Five linear models were examined: two models using a dedicated genetic software program (ASREML) with the capacity to specify A or other pedigree-related functions, and three models with the doubled dataset and a parent (or sire) linear model (ASREML, SAS Proc Mixed, and R lme4). Results: The variance components, genetic parameters, and BLUPs of the parental breeding values, progeny breeding values, and full-sib family-specific combining abilities were compared. Genetic parameter estimates were essentially the same across all the analyses (e.g., the heritability ranged from h2 = 0.220 to 0.223, and the proportion of dominance variance ranged from d2 = 0.057 to 0.058). The correlations between the BLUPs from the baseline analysis (ASREML with an individual tree model) and the doubled-dataset/parent models using SAS Proc Mixed or R lme4 were never lower than R = 0.99997. Conclusions: The algorithm can be useful for analysts who need to analyze full-sib genetic datasets and who are familiar with general-purpose statistical packages, but less familiar with or lacking access to other software.
Harmonized Classification of Forest Types in the Iberian Peninsula Based on National Forest Inventories
Authors: Nunes Leónia , Moreno Mauro , Alberdi Iciar , Álvarez-González Juan Gabriel , Godinho-Ferreira Paulo , Mazzoleni Stefano , Castro Rego Francisco
Published: November 2020

National Forest Inventories (NFIs) collect and provide a large amount of information regarding the forest volume, carbon stocks, vitality, biodiversity, non-wood forest products and their changes. Forest stands variables data are paramount to understanding their composition, especially on those related with understory characteristics and the coverage of species according to canopy layers; they are essential to assess biodiversity and to support forest management. At the same time, these inventories allow the development of harmonized forest descriptions beyond the national scale. This study aims to develop a homogeneous characterization of the Iberian Peninsula’s forests, in order to classify and identify the forest types. For this purpose, harmonized data from NFIs of Portugal and Spain were used to assess the composition of species, dominance and the percentage of cover for each species in a vertical space defined by seven canopy layers. Using the “K-means” clustering algorithm, a set of clusters was identified and georeferenced using forest polygons from land use and cover maps of both countries. The interpretation and description of the clusters lead to the establishment of 28 forest types that characterize all of the Iberian Peninsula forests. Each forest area has been described through one of the forest types and their relation with other ecological characteristics of the stands was analyzed. Shrubs formations are generally widely distributed in the forest area of the Iberian Peninsula, however their abundance in terms of cover is lower in comparison with tree species. Around 71% of the forest types are dominated by trees, mainly species from the genera Pinus and Quercus, and 21% are dominated by shrub formations with species of Ulex spp., Cytisus spp., and Cistus spp. The Quercus ilex s.l. L. and Pinus pinaster Aiton are the common species of importance for both NFIs. The results represent a powerful and homogenous multi-use tool describing the Iberian Peninsula’s forestlands with applications on landscape analysis, forest management and conservation. This information can be used for comparisons at larger scales, allowing cross-border analysis in relation to various aspects, such as hazards and wildfires, as well as management and conservation of forest biodiversity. The developed method is adaptable to an updated dataset from more recent NFIs and to other study areas.
Heat Treatment of Basalt Fiber Reinforced Expanded Clay Concrete with Increased Strength for Cast-In-Situ Construction
Authors: Kharun Makhmud , Klyuev Sergey , Koroteev Dmitry , Chiadighikaobi Paschal C. , Fediuk Roman , Olisov Andrej , Vatin Nikolai , Alfimova Nataliya
Published: November 2020

Expanded clay concrete (ECC) is a promising structural material for buildings due to its light weight and heat- and sound-insulating properties. Adding basalt fibers (BFs) in ECC reduces its brittleness and enhances its mechanical properties. The heat treatment (HT) of BF-reinforced ECC can significantly accelerate the strength growth during cast-in-situ construction, which allows the reduction of the turnover of the formwork and the construction period, as well as leading to lower construction costs. This paper presents an HT technology for load-bearing structures, containing a BF-reinforced ECC mix and using infrared rays for cast-in-situ construction. The issue of the strength growth of BF-reinforced ECC during HT has been studied. Microsilica and fly ash were added to the ECC mix to obtain a compressive strength of more than 20 MPa. Four different mixes of ECC with chopped BFs in the ratios of 1:0, 1:0.0045, 1:0.009 and 1:0.012 by weight of cement were studied. Test specimens were heated by infrared rays for 7, 9, 11, 13, 16 and 24 h. Then, the heat-treated specimens were tested for compressive strength after 0.5, 4, 12 and 24 h cooling periods. The analysis and evaluation of the experimental data were carried out based on probability theory and mathematical statistics. Mathematical models are proposed for forecasting the strength growth of BF-reinforced ECC during cast-in-situ construction.
Polymers and Plastrons in Parallel Yield Enhanced Turbulent Drag Reduction
Authors: Rajappan Anoop , McKinley Gareth H.
Published: November 2020

Despite polymer additives and superhydrophobic walls being well known as stand-alone methods for frictional drag reduction in turbulent flows, the possibility of employing them simultaneously in an additive fashion has remained essentially unexplored. Through experimental friction measurements in turbulent Taylor–Couette flow, we show that the two techniques may indeed be combined favorably to generate enhanced levels of frictional drag reduction in wall-bounded turbulence. We further propose an additive expression in Prandtl–von Kármán variables that enables us to quantitatively estimate the magnitude of this cooperative drag reduction effect for small concentrations of dissolved polymer.
Potential of Propolis Extract as a Natural Antioxidant and Antimicrobial in Gelatin Films Applied to Rainbow Trout (Oncorhynchus mykiss) Fillets
Authors: Ucak Ilknur , Khalily Rowida , Carrillo Celia , Tomasevic Igor , Barba Francisco J.
Published: November 2020

Usage of edible films and coatings alone or incorporated with natural extracts are a new approach to preservation and packaging of food. In this study, therefore, the microbiological, chemical quality, and sensorial changes of rainbow trout fillets coated with gelatin films supplemented with propolis extract (PE) (2, 8, 16%), as a source of polyphenols, were determined during 15 days of refrigerated storage (4 ± 1 °C). According to peroxide value (PV) and thiobarbituric acid reactive substances (TBARS) assays, lipid oxidation was delayed in the fillets coated with gelatin films incorporated with PE comparing with the control and gelatin-coated (without PE) fillets. The total volatile basic nitrogen (TVB-N) value of rainbow trout fillets showed an increase in all groups at the end of storage, observing the lowest values in the fillets coated with gelatin films prepared with 16% PE. Gelatin films enriched with PE had great inhibitory effects on the microbial growth in rainbow trout fillets. The addition of PE enhanced the effectiveness of gelatin films and delayed the lipid oxidation and sensory and microbial deterioration in trout fillets coated with these films. Thus, PE can be recommended to be used as a natural antioxidant and antimicrobial additive with gelatin films to maintain rainbow trout fillet quality.
Mycotoxin Occurrence, Exposure and Health Implications in Infants and Young Children in Sub-Saharan Africa: A Review
Authors: Adaku Chilaka Cynthia , Mally Angela
Published: November 2020

Infants and young children (IYC) remain the most vulnerable population group to environmental hazards worldwide, especially in economically developing regions such as sub-Saharan Africa (SSA). As a result, several governmental and non-governmental institutions including health, environmental and food safety networks and researchers have been proactive toward protecting this group. Mycotoxins, toxic secondary fungal metabolites, contribute largely to the health risks of this young population. In SSA, the scenario is worsened by socioeconomic status, poor agricultural and storage practices, and low level of awareness, as well as the non-establishment and lack of enforcement of regulatory limits in the region. Studies have revealed mycotoxin occurrence in breast milk and other weaning foods. Of concern is the early exposure of infants to mycotoxins through transplacental transfer and breast milk as a consequence of maternal exposure, which may result in adverse health effects. The current paper presents an overview of mycotoxin occurrence in foods intended for IYC in SSA. It discusses the imperative evidence of mycotoxin exposure of this population group in SSA, taking into account consumption data and the occurrence of mycotoxins in food, as well as biomonitoring approaches. Additionally, it discusses the health implications associated with IYC exposure to mycotoxins in SSA.
A Meta-Analysis of the Effectiveness of High, Medium, and Low Voltage Electrical Stimulation on the Meat Quality of Small Ruminants
Authors: Ravindranathan Archana , Dunshea Frank , Warner Robyn , Leury Brian , Ha Minh , Chauhan Surinder
Published: November 2020

The current study is a meta-analysis of the effects of electrical stimulation (ES, n = 28 papers) with different voltages combined with different ageing periods (0–3, 4–7, and 8–14 days) on the meat quality of small ruminants. A comprehensive search for published studies on meat quality of small ruminants investigating the application of low, medium, and high voltage electrical stimulation, was performed using Google Scholar, ScienceDirect, PubMed, and Scopus databases. Forest plots, funnel plots, and other statistical tools and tests were used in the study to analyze the results. Electrical stimulation significantly reduced ultimate pH (p < 0.001), Warner–Bratzler shear force (WBSF) (p < 0.001), cooking loss (p < 0.05), and purge loss (p < 0.001). In addition, sarcomere length (p < 0.01), myofibrillar-fragmentation index (MFI) (p < 0.001), and color (L*, a*, b*) (p < 0.001) showed higher values in meat subjected to ES as compared with the control group. In conclusion, the meta-analysis revealed statistical proof of beneficial effects of ES on meat quality of small ruminants in terms of ultimate pH, tenderness, enhanced proteolysis, and higher colorimetric values.
New Directions in Behavioral Game Theory: Introduction to the Special Issue
Authors: Golman Russell
Published: November 2020

Behavioral game theory accounts for how people actually make strategic decisions by incorporating social utility, limited iterated reasoning, and learning [...]
“The Ghost Language Which Passes between the Generations”: Transgenerational Memories and Limit-Case Narratives in Lisa Appignanesi’s Losing the Dead and The Memory Man
Authors: Pellicer-Ortín Silvia
Published: November 2020

This article aims to uncover the tensions and connections between Lisa Appignanesi’s autobiographical work Losing the Dead (1999) and her novel The Memory Man (2004) and to point out that, in spite of belonging to different genres, they share several formal, thematic, and structural features. By applying close-reading and narratological tools and drawing on relevant theories within Trauma, Memory, and Holocaust Studies, I would like to demonstrate that both works can be defined as limit-case narratives on the grounds that they blur literary genres, fuse testimonial and narrative layers, include metatextual references to memory and trauma, and represent and perform the transgenerational encounter with traumatic memories. Moreover, Appignanesi’s creations will be contextualised within the trend of hybrid life-writing narratives developed by contemporary British-Jewish women writers. Accordingly, these authors are contributing to the expansion of innovative liminal autobiographical and fictional practices that try to represent what it means to be a Jew, a migrant, and an inheritor of traumatic experiences in the post-Holocaust world. Finally, I launch a further reflection on the generic hybridisation characterising those contemporary narratives based on the negotiation of transgenerational memories, which will be read as a fruitful strategy to problematize the conflicts created when the representation of the self and (family) trauma overlap.
A Methodological Quality Assessment of Meta-Analysis Studies in Dance Therapy Using AMSTAR and AMSTAR 2
Authors: Kim Hye-Ryeon , Choi Chang-Hwan , Jo Eunhye
Published: November 2020

Although earlier meta-analysis studies have provided evidence-based information useful for decision-making, debate regarding their quality continues. This study aimed to evaluate the quality of meta-analysis studies in the field of dance therapy (DT) using the Assessment of Multiple Systematic Reviews (AMSTAR) and AMSTAR 2 assessment tools. Meta-analysis studies on DT were collected from various databases. Seven meta-analysis studies were selected for this study. Our findings showed that the quality level of the meta-analysis studies related to DT was “High” on the AMSTAR evaluation, but their quality decreased to “Low” on the AMSTAR 2 evaluation. Moreover, using AMSTAR 2, 71.43% of the studies fell within the category of “Moderate” or below. There was no statistically significant difference in the quality scores of the characteristics of these studies. Our results suggest that (1) education on meta-analysis guidelines is required to improve the quality of DT-related meta-analysis studies, and (2) methodological caution is warranted, since different outcomes in evaluation scores for each tool may be obtained when using AMSTAR and AMSTAR 2. Based on this study, it is expected that common and specific guidelines for meta-analysis in DT can be established.
Similar Quality of Life and Safety in Patients Receiving Inpatient or Outpatient Chemotherapy: A Focus on Esophageal Squamous Cell Carcinoma
Authors: Chen Yen-Hao , Chen Su-Wei , Lu Hung-I , Lo Chien-Ming , Li Shau-Hsuan
Published: November 2020

Quality of life (QoL) is a particularly important issue for cancer patients. This study was designed to investigate the differences in QoL in esophageal squamous cell carcinoma (ESCC) patients who underwent inpatient chemotherapy (IPCT) or outpatient chemotherapy (OPCT). A total of 107 ESCC patients were enrolled, including 53 patients in the IPCT group and 54 patients in the OPCT group. The widely used and well-validated instruments European Organization for Research and Treatment of Cancer Quality of Life Questionnaire Core 30 Items (EORTC QLQ-C30) and Oesophageal Cancer Module (EORTC QLQ-OES18) were used to examine the QoL of the two groups. In addition, the differences in adverse events (AEs) were evaluated. The results of QLQ C-30 analysis showed that mean global quality of life scores were similar between IPCT and OPCT groups, as were functional and symptom scales. There were no significant differences in the functional and symptom scales in the analysis of QLQ OES18 either. Most AEs of chemotherapy were grades 1–2, and the majority of patients tolerated the side effects; no statistically significant difference in AEs between these two groups was mentioned. Our study suggests that the health-related QoL and adverse events in ESCC patients who received IPCT or OPCT are similar. OPCT is reasonable and safe in clinical practice.
The Motion of the Italian National Bioethics Committee on Aggressive Treatment towards Children with Limited Life Expectancy
Authors: Bolcato Matteo , Russo Marianna , Feola Alessandro , Della Pietra Bruno , Tettamanti Camilla , Bonsignore Alessandro , Ciliberti Rosagemma , Rodriguez Daniele , Aprile Anna
Published: November 2020

The motion of the Italian National Bioethics Committee entitled “Aggressive treatment or therapeutic obstinacy on young children with limited life expectancy” comprises a premise that rejects therapeutic obstinacy and makes 12 recommendations. Recommendation no. 1 states the general rules: it ascribes a cardinal role to a shared care plan, it supports pain management therapy and pain relief, it opposes ineffective and disproportionate clinical treatment and defensive medicine. The other recommendations are correlated to the enacting of a national law establishing clinical ethics committees in paediatric hospitals; participation of parents and their fiduciaries in the decision-making processes; recourse to courts only as extrema ratio in the event of irremediable disagreement between the medical team and the family members; accompaniment at the end of life also through continuous deep sedation combined with pain therapy; access to palliative care; the need to reinforce research on pain and suffering in children; clinical trials and research studies conducted in children; the training of doctors, healthcare personnel and psychologists, to support parents in emotional and practical terms; the facilitation of the closeness of parents to children in extremely precarious clinical conditions; the relevant role of the associations of parents of sick children. Comments are made, in particular, about the innovative recommendations respectively relating to the adoption of care planning, the establishment, by law, of clinical ethics committees in paediatric hospitals and the limitation of recourse to courts—only as extrema ratio—in the event of irremediable disagreement between the medical team and the family members.
Development and Validation of a Chinese Version of a Professional Identity Scale for Healthcare Students and Professionals
Authors: Liao Hung-Chang , Wang Ya-huei
Published: November 2020

This study was intended to develop a comprehensive and psychometrically adequate professional identity scale for healthcare students and professionals based on Taiwanese cultural contexts. In order to elicit a more consistent result of the psychometric indices of the newly developed scale, the study adopted a combination of exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) to examine the consistency of the scale factors. In a pilot study of 562 randomly selected healthcare students and professionals, the EFA yielded a 33-item four-factor model with the terms “professional commitment & devotion” (16 items; 47.33% of variance), “emotional identification & belongingness” (7 items; 9.72% of variance), “professional goals & values” (5 items; 8.17% of variance), and “self-fulfillment & retention tendency” (5 items; 3.38% of variance). The CFA yielded an 18-item four-factor model with good or even excellent fit to the data, where the χ2/df ratio = 1.138, Tucker Lewis index (TLI) = 0.997, comparative fit index (CFI) = 0.997, and root mean square of approximation (RMSEA) = 0.016. The convergent validity and discriminant validities were also conducted to test the feasibility of the Professional Identity Scale for Healthcare Students and Professionals (PIS-HSP) scale. For the EFA model, the Cronbach’s alphas for the four factors and the overall scale ranged from 0.84 to 0.96; for the CFA model, the Cronbach’s alphas and composite reliabilities were, respectively, in the ranges of 0.78–0.93 and 0.78–0.97, demonstrating satisfactory reliabilities. The results proved that the developed PIS-HSP can be a reliable measurement tool to assess professional identity for healthcare students and professionals.
Differences in the Potential Accessibility of Home-Based Healthcare Services among Different Groups of Older Adults: A Case from Shaanxi Province, China
Authors: Wang Lijian , Di Xiaodong , Yang Liu , Dai Xiuliang
Published: November 2020

With the increase of the aging population and the lack of family care, home-based healthcare services have gradually become the main model to cope with aging, so local governments have invested heavily in the construction of home-based healthcare services. However, healthcare services still have problems such as low resource utilization and imbalanced development. The reason is that the supply and demand of healthcare services are not matched and the potential accessibility is low. Therefore, based on the supply and demand of healthcare services, this article pulls out the spatial and social factors that affect the potential accessibility, and tests the influence of individual factors on the potential accessibility among different groups of older adults. It is found that the perceived vulnerability of the older adults will reduce the potential accessibility of healthcare services. The psychosocial status, income and education level with the willingness to use healthcare services of the older adults are directly proportional, while residence has a negative impact on the potential accessibility. Finally, based on this finding, this article puts forward feasible suggestions from the perspective of policy content, publicity, and implementation.
Prediction and Potential Spatially Explicit Spread of COVID-19 in Mexico’s Megacity North Periphery
Authors: Hernández-Flores Maria de la Luz , Escobar-Sánchez Jair , Paredes-Zarco Jesús Eduardo , Franyuti Kelly Giorgio Alberto , Carranza-Ramírez Lamán
Published: November 2020

The novel COVID-19, detected in Wuhan, China, has reached almost every city across the globe, and researchers from many countries have used several epidemiologic models to describe the epidemic trends. In this context, it is also important to know the geographic extent of the infected population. Following this approach, a Gumpertz model was adapted with official data from the state of Hidalgo, Mexico, in order to estimate the people infected during this COVID-19 pandemic. We found, based on the adjusted data, the highest value in infected people according to official and theoretical data. Furthermore, using a geographical analysis based on geostatistical measures related to density of demographic and economic data, traffic level and geolocation, raster files were generated to estimate probability of coronavirus cases occurrence using the areas where the contagion may occur. We also distributed the maximum contagion obtained by the epidemic model, using these raster files, and a regression model to weight factors according their importance. Based on this estimated distribution, we found that most of the infected people were located in the southern border, a trend related to the economic strip in the southern part of Hidalgo State, associated with its vicinity to the Megacity of Mexico.
Levels of Anti-Citrullinated Protein Antibodies and Rheumatoid Factor, Including IgA Isotypes, and Articular Manifestations in Ulcerative Colitis and Crohn’s Disease
Authors: Janssen Koen M. J. , Hop Hilde , Vissink Arjan , Dijkstra Gerard , de Smit Menke J. , Brouwer Elisabeth , Westra Johanna
Published: November 2020

Systemic presence of arthritis autoantibodies (AAb) is specific for rheumatoid arthritis (RA). AAb initiation might be triggered by chronic mucosal inflammation, such as in inflammatory bowel disease (IBD). We assessed the prevalence of anti-citrullinated protein antibodies (ACPA) and rheumatoid factor (RF) in ulcerative colitis (UC) and Crohn’s disease (CD) patients, with regard to the prevalence of joint complaints in AAb+ versus AAb− IBD patients. RA patients and healthy subjects (HC) served as controls. Serum was collected from 226 UC, 165 CD and 86 RA patients, and 36 HCs. One-hundred-and-ten UC (48.7%) and 76 CD (46.1%) patients were seropositive for at least one autoantibody, compared to 4 (13.9%) HCs and 81 (94.2%) RA patients. Eighty-three (37%) UC and 52 (32%) CD patients were seropositive for the anti-cyclic citrullinated protein antibody (anti-CCP2) of the immunoglobulin A type (IgA anti-CCP2), compared to 1 (2.8%) HC and 64 (74%) RA patients. RF of the immunoglobulin G type (IgG RF) and IgA RF seropositivity in UC and CD patients was comparable to HCs and low compared to RA patients. Arthralgia was reported by 34 (18.7%) UC and 50 (33.1%) CD patients, but presence of arthralgia was not increased in AAb+ patients. AAbs are frequently present in IBD patients, supporting the hypothesis that inflammation of intestinal mucosa induces low systemic levels of ACPA.
The Combination of a Diversified Intake of Carbohydrates and Fats and Supplementation of Vitamin D in a Diet Does Not Affect the Levels of Hormones (Testosterone, Estradiol, and Cortisol) in Men Practicing Strength Training for the Duration of 12 Weeks
Authors: Wrzosek Michał , Woźniak Jakub , Włodarek Dariusz
Published: November 2020

This study aims to verify the extent to which a diversification of carbohydrates and fats intake in a diet, together with the reduction in vitamin D deficiency, impact the levels of hormones (testosterone, estradiol, cortisol) and Sex Hormone Binding Globulin (SHGB) in men doing strength training. The research involved 55 men aged 19–35. The participants were divided into two groups following two interventional diets for 12 weeks: high-fat diet (LCHF) or high-carb diet (LFHC), which were applied to satisfy the caloric requirements of each participant. Moreover, vitamin D supplementation was included. Moreover, both before and after following interventional diets, the level of hormones in participant’s blood was examined. After 12 weeks of following interventional diets in both groups, no changes of the levels of testosterone and estradiol, as well as SHGB, were found. The cortisol level in both groups decreased; however, only in the LFHC group was the change statistically significant (p = 0.03) and amounted to −3.5% (the cortisol level on an empty stomach in this group decreased from 14.17 ± 3.35 to 13.93 ± 2.63 mcg/dl). In both groups, the supplementation of vitamin D brought about a significant change in the level of vitamin D metabolite (25 (OH) D) (p = 0.01). In the LCHF group, the level of metabolite increased by 95%, and by 58.3% in the LFHC group. The increase in vitamin D metabolite in blood was higher in the LCHF group than the LFHC group, which might have resulted from the supplementation of this vitamin.
Post-Orthodontic Lower Incisors Recessions: Combined Periodontic and Orthodontic Approach
Authors: Beitlitum Ilan , Barzilay Vered , Rayyan Fatma , Sebaoun Alon , Sarig Rachel
Published: November 2020

The bonded lingual retainer (BLR) is considered a favorable choice for retaining lower incisors’ alignment post-orthodontic treatment; however, it may cause some unwanted effects such as inadvertent tooth movement and torque changes. These often result in gingival recession (Miller class III-type) with exposure of the root surface, which compromises the esthetics and hinders the comfort of the patient. Fifteen post-orthodontic patients presenting Miller class III-type recessions with BLR were examined. Two protocols were used: the first included the removal of the BLR prior to surgery and the second included only a surgical approach. All patients underwent the same surgery of a modified tunnel double papilla procedure for root coverage. The gingival recession was measured using a dental probe before, and three to six months post-surgery. The average improvement in recession depth was significantly greater (p = 0.008) for the protocol that included removal of the BLR (4.0 ± 0.83 mm) with an improvement of 87.2% as compared to the second protocol that showed an improvement of 43.8% (1.88 ± 1.29 mm). Removing the BLR prior to surgery is beneficial for predictable root coverage in post-orthodontic Miller class III recessions.
“Follow the Whistle: Physical Activity Is Calling You”: Evaluation of Implementation and Impact of a Portuguese Nationwide Mass Media Campaign to Promote Physical Activity
Authors: Silva Marlene Nunes , Godinho Cristina , Salavisa Marta , Owen Katherine , Santos Rute , Silva Catarina Santos , Mendes Romeu , Teixeira Pedro J. , Freitas Graça , Bauman Adrian
Published: November 2020

To raise perceived capability (C), opportunity (O) and motivation (M) for physical activity (PA) behaviour (B) among adults, the Portuguese Directorate-General of Health developed a mass media campaign named “Follow the Whistle”, based on behaviour change theory and social marketing principles. Comprehensive formative and process evaluation suggests this media-led campaign used best-practice principles. The campaign adopted a population-wide approach, had clear behavioural goals, and clear multi-strategy implementation. We assessed campaign awareness and initial impact using pre (n = 878, 57% women) and post-campaign (n = 1319, 58% women) independent adult population samples via an online questionnaire, comprising socio-demographic factors, campaign awareness and recall, and psychosocial and behavioural measures linked to the COM-B model. PA was assessed with IPAQ and the Activity Choice Index. The post-campaign recall was typical of levels following national campaigns (24%). Post-campaign measures were higher for key theory-based targets (all p < 0.05), namely self-efficacy, perceived opportunities to be more active and intrinsic motivation. The impact on social norms and self-efficacy was moderated by campaign awareness. Concerning PA, effects were found for vigorous activity (p < 0.01), but not for incidental activity. Overall the campaign impacted key theory-based intermediate outcomes, but did not influence incidental activity, which highlights the need for sustained and repeated campaign efforts.
Prototype Development of an Expert System of Computerized Clinical Guidelines for COVID-19 Diagnosis and Management in Saudi Arabia
Authors: Banjar Haneen Reda , Alkhatabi Heba , Alganmi Nofe , Almouhana Ghaidaa Ibraheem
Published: November 2020

The increasing number of COVID-19 patients has increased health care professionals’ workloads, making the management of dynamic patient information in a timely and comprehensive manner difficult and sometimes impossible. Compounding this problem is a lack of health care professionals and trained medical staff to handle the increased number of patients. Although Saudi Arabia has recently improved the quality of its health services, there is still no suitable intelligent system that can help health practitioners follow the clinical guidelines and automated risk assessment and treatment plan remotely, which would allow for the effective follow-up of patients of COVID-19. The proposed system includes five sub-systems: an information management system, a knowledge-based expert system, adaptive learning, a notification and follow-up system, and a mobile tracker system. This study shows that, to control epidemics, there is a method to overcome the shortage of specialists in the management of infections in Saudi Arabia, both today and in the future. The availability of computerized clinical guidance and an up-to-date knowledge base play a role in Saudi health organizations, which may not have to constantly train their physician staff and may no longer have to rely on international experts, since the expert system can offer clinicians all the information necessary to treat their patients.
Comparison of Post-fire Patterns in Brazilian Savanna and Tropical Forest from Remote Sensing Time Series
Authors: Santana Níckolas Castro , Júnior Osmar Abílio de Carvalho , Gomes Roberto Arnaldo Trancoso , Fontes Guimarães Renato
Published: November 2020

Monitoring of fire-related changes is essential to understand vegetation dynamics in the medium and long term. Remote sensing time series allows estimating biophysical variables of terrestrial vegetation and interference by extreme fires. This research evaluated fire recurrence in the Amazon and Cerrado regions, using Moderate Resolution Imaging Spectroradiometer (MODIS) albedo time series, enhanced vegetation index (EVI), gross primary productivity (GPP), and surface temperature. The annual aggregated time series (AAT) method recognized each pixel’s slope trend in the 2001–2016 period and its statistical significance. A comparison of time trends of EVI, GPP, and surface temperature with total fire recurrence indicates that time trends in vegetation are highly affected by high fire recurrence scenarios (R2 between 0.52 and 0.90). The fire recurrence and the albedo’s persistent changes do not have a consistent relationship. Areas with the biggest evaluated changes may increase up to 0.25 Kelvin/Year at surface temperature and decrease up to −0.012 EVI/year in vegetation index. Although savannas are resistant to low severity fires, fire regime and forest structure changes tend to make vegetation more vulnerable to wildfires, reducing their regeneration capacity. In the Amazon area, protection of forests in conservation units and indigenous lands helped in the low occurrence of fires in these sensitive areas, resulting in positive vegetation index trends.
Monoclonal Antibodies to Treat Multiple Myeloma: A Dream Come True
Authors: D’Agostino Mattia , Innorcia Salvatore , Boccadoro Mario , Bringhen Sara
Published: November 2020

Immunotherapy is increasingly used in the treatment of multiple myeloma (MM). Monoclonal antibodies (mAbs) are safe and effective ways to elicit immunotherapeutic responses. In 2015, daratumumab has become the first mAb approved by the Food and Drug Administration for clinical use in MM and, in the last 5 years, a lot of clinical and preclinical research has been done to optimize the use of this drug class. Currently, mAbs have already become part of standard-of-care combinations for the treatment of relapsed/refractory MM and very soon they will also be used in the frontline setting. The success of simple mAbs (‘naked mAbs’) prompted the development of new types of molecules. Antibody–drug conjugates (ADCs) are tumor-targeting mAbs that release a cytotoxic payload into the tumor cells upon antigen binding in order to destroy them. Bispecific antibodies (BiAbs) are mAbs simultaneously targeting a tumor-associated antigen and an immune cell-associated antigen in order to redirect the immune cell cytotoxicity against the tumor cell. These different constructs produced solid preclinical data and promising clinical data in phase I/II trials. The aim of this review article is to summarize all the recent developments in the field, including data on naked mAbs, ADCs and BiAbs.
2-Hydroxy-4-Methylbenzoic Anhydride Inhibits Neuroinflammation in Cellular and Experimental Animal Models of Parkinson’s Disease
Authors: Song Soo-Yeol , Kim In-Su , Koppula Sushruta , Park Ju-Young , Kim Byung-Wook , Yoon Sung-Hwa , Choi Dong-Kug
Published: November 2020

Microglia-mediated neuroinflammation is one of the key mechanisms involved in acute brain injury and chronic neurodegeneration. This study investigated the inhibitory effects of 2-hydroxy-4-methylbenzoic anhydride (HMA), a novel synthetic derivative of HTB (3-hydroxy-4-trifluoromethylbenzoic acid) on neuroinflammation and underlying mechanisms in activated microglia in vitro and an in vivo mouse model of Parkinson’s disease (PD). In vitro studies revealed that HMA significantly inhibited lipopolysaccharide (LPS)-stimulated excessive release of nitric oxide (NO) in a concentration dependent manner. In addition, HMA significantly suppressed both inducible NO synthase and cyclooxygenase-2 (COX-2) at the mRNA and protein levels in LPS-stimulated BV-2 microglia cells. Moreover, HMA significantly inhibited the proinflammatory cytokines such as interleukin (IL)-1beta, IL-6, and tumor necrosis factor-alpha in LPS-stimulated BV-2 microglial cells. Furthermore, mechanistic studies ensured that the potent anti-neuroinflammatory effects of HMA (0.1, 1.0, and 10 μM) were mediated by phosphorylation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) in LPS-stimulated BV-2 cells. In vivo evaluations revealed that intraperitoneal administration of potent neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 20 mg/kg, four times a 1 day) in mice resulted in activation of microglia in the brain in association with severe behavioral deficits as assessed using a pole test. However, prevention of microglial activation and attenuation of Parkinson’s disease (PD)-like behavioral changes was obtained by oral administration of HMA (30 mg/kg) for 14 days. Considering the overall results, our study showed that HMA exhibited strong anti-neuroinflammatory effects at lower concentrations than its parent compound. Further work is warranted in other animal and genetic models of PD for evaluating the efficacy of HMA to develop a potential therapeutic agent in the treatment of microglia-mediated neuroinflammatory disorders, including PD.
From Grafts to Human Bioengineered Vascularized Skin Substitutes
Authors: Oualla-Bachiri Wasima , Fernández-González Ana , Quiñones-Vico María I. , Arias-Santiago Salvador
Published: November 2020

The skin plays an important role in the maintenance of the human’s body physiological homeostasis. It acts as a coverage that protects against infective microorganism or biomechanical impacts. Skin is also implied in thermal regulation and fluid balance. However, skin can suffer several damages that impede normal wound-healing responses and lead to chronic wounds. Since the use of autografts, allografts, and xenografts present source limitations and intense rejection associated problems, bioengineered artificial skin substitutes (BASS) have emerged as a promising solution to address these problems. Despite this, currently available skin substitutes have many drawbacks, and an ideal skin substitute has not been developed yet. The advances that have been produced on tissue engineering techniques have enabled improving and developing new arising skin substitutes. The aim of this review is to outline these advances, including commercially available skin substitutes, to finally focus on future tissue engineering perspectives leading to the creation of autologous prevascularized skin equivalents with a hypodermal-like layer to achieve an exemplary skin substitute that fulfills all the biological characteristics of native skin and contributes to wound healing.
Enhanced Collagen Deposition in the Duodenum of Patients with Hyaline Fibromatosis Syndrome and Protein Losing Enteropathy
Authors: van Rijn Jorik M. , Werner Lael , Aydemir Yusuf , Spronck Joey M.A. , Pode-Shakked Ben , van Hoesel Marliek , Shimshoni Elee , Polak-Charcon Sylvie , Talmi Liron , Eren Makbule , Weiss Batia , H.J. Houwen Roderick , Barshack Iris , Somech Raz , Nieuwenhuis Edward E.S. , Sagi Irit , Raas-Rothschild Annick , Middendorp Sabine , Shouval Dror S.
Published: November 2020

Hyaline fibromatosis syndrome (HFS), resulting from ANTXR2 mutations, is an ultra-rare disease that causes intestinal lymphangiectasia and protein-losing enteropathy (PLE). The mechanisms leading to the gastrointestinal phenotype in these patients are not well defined. We present two patients with congenital diarrhea, severe PLE and unique clinical features resulting from deleterious ANTXR2 mutations. Intestinal organoids were generated from one of the patients, along with CRISPR-Cas9 ANTXR2 knockout, and compared with organoids from two healthy controls. The ANTXR2-deficient organoids displayed normal growth and polarity, compared to controls. Using an anthrax-toxin assay we showed that the c.155C>T mutation causes loss-of-function of ANTXR2 protein. An intrinsic defect of monolayer formation in patient-derived or ANTXR2KO organoids was not apparent, suggesting normal epithelial function. However, electron microscopy and second harmonic generation imaging showed abnormal collagen deposition in duodenal samples of these patients. Specifically, collagen VI, which is known to bind ANTXR2, was highly expressed in the duodenum of these patients. In conclusion, despite resistance to anthrax-toxin, epithelial cell function, and specifically monolayer formation, is intact in patients with HFS. Nevertheless, loss of ANTXR2-mediated signaling leads to collagen VI accumulation in the duodenum and abnormal extracellular matrix composition, which likely plays a role in development of PLE.
Implications of Splicing Alterations in the Onset and Phenotypic Variability of a Family with Subclinical Manifestation of Peutz–Jeghers Syndrome: Bioinformatic and Molecular Evidence
Authors: Cerasuolo Andrea , Cammarota Francesca , Duraturo Francesca , Staiano Annamaria , Martinelli Massimo , Miele Erasmo , Izzo Paola , De Rosa Marina
Published: November 2020

Peutz–Jeghers Syndrome (PJS) is an autosomal dominant pre-cancerous disorder caused in 80–90% of cases by germline mutations in the tumor suppressor gene STK11. We performed a genetic test of the STK11 gene in two Italian young sisters suspected of PJS, since they showed pathognomonic café au lait spots in absence of other symptoms and familiarity. Sequencing of all exons of STK11 gene and other 8 genes, suggested to be involved in hamartomatous syndromes, (PTEN, BMPR1A, SDHB, SDHD, SMAD4, AKT1, ENG, PIK3CA) led to the identification in both the probands of a novel germline silent mutation named c.597 G>A, hitting the last nucleotide of exon 4. Interestingly, genetic testing of the two probands’ parents showed that their unaffected father was carrier of this mutation. Moreover, he carried a second intronic substitution named c.465-51 T>C (rs2075606) which was not inherited by his daughters. We also observed that all the family members carrying the c.597 G>A mutation presented an aberrant splice variant of STK11 mRNA lacking exon 4. Furthermore, in silico analysis of c.465-51 T>C substitution showed that it may activate an Enhancer Splicing Element. Finally, qRT-PCR analysis of STK11 expression levels showed a slight downregulation of the wild type allele in the father and a 2-fold downregulation in the probands compared to the unaffected mother. Our results have led the hypothesis that the c.465-51 T>C intronic variant, which segregates with the wild type allele, could increase the splicing effectiveness of STK11 wild-type allele and compensate the side effect of the c.597 G>A splicing mutation, being responsible for the phenotypic variability observed within this family. This finding highlight the importance of RNA analysis in genetic testing, remarking that silent DNA variant can often be splicing variant involved in disease onset and progression. The identification of these variants has a crucial role to ensure an appropriate follow-up and cancer prevention in at-risk individuals.