bluuebunny/crossref_metadata_2025 huggingface dataset:
Comprehensive Approach for the Genetic Diagnosis of Patients with Waardenburg Syndrome
Authors: Buonfiglio Paula Inés , Izquierdo Agustín , Pace Mariela Vanina , Grinberg Sofia , Lotersztein Vanesa , Brun Paloma , Bruque Carlos David , Elgoyhen Ana Belén , Dalamón Viviana
Published: August 2024

Waardenburg syndrome (WS) is a common genetic cause of syndromic hearing loss, accounting for 2–5% of congenital cases. It is characterized by hearing impairment and pigmentation abnormalities in the skin, hair, and eyes. Seven genes are associated with WS: PAX3, MITF, EDNRB, EDN3, SOX10, KITLG, and SNAI2. This study investigates the genetic causes of WS in three familial cases. Whole-exome sequencing (WES) was performed to identify single nucleotide variants (SNVs). Copy number variants (CNVs) were analyzed from the WES raw data and through multiplex ligation-dependent probe amplification (MLPA). The study identified one pathogenic SNV and two novel CNVs, corresponding to type I and type II WS patterns in the three families. The SNV, a nonsense variant (c.1198C>T p.Arg400*), was found in MITF and segregated in the affected father. The two CNVs were a deletion of exon 5 in PAX3 in a family with two affected members and a large novel deletion comprising seven genes, including SOX10, in a family with three affected members. These findings confirmed a WS diagnosis through genetic testing. The study emphasizes the importance of integrating multiple genetic testing approaches for accurate and reliable diagnosis, highlighting their role in improving patient management and providing tailored genetic counseling.
Head–Shaft Angle Influences Isometric Shoulder Strength Levels after Intramedullary Nailing of Proximal Humerus Fractures: A Pilot Study
Authors: Grimme Sebastian , Bail Hermann Josef , Rüther Johannes , Millrose Michael , Biber Roland , Gesslein Markus , Willauschus Maximilian
Published: August 2024

Background: Proximal humerus fractures are common fractures of the elderly population which can lead to long-term compromise of a patient’s shoulder function. Closed reduction and internal fixation with intramedullary nailing is a well-established surgical technique yielding good outcomes, as perceived by patients, obtained via Patient-Reported Outcome Measures, and objectified by clinical shoulder testing. Apart from conventional range-of-motion testing and clinical shoulder tests, strength testing of the shoulder is a yet-neglected but meaningful and standardizable outcome parameter. In this study, isometric shoulder strength is evaluated in relation to fracture morphology/postoperative reduction quality as well as with patient-reported outcomes. Methods: 25 patients (mean age 73.2 ± 10.5 years) underwent isometrics strength-testing of the shoulder joint in the scapular plane (abduction) as well as in the sagittal plane (flexion) as well as hand-grip strength-testing at 4.5 ± 1.88 years follow-up. Pre- and postoperative radiographs were analysed. Patients completed ASES and CMS questionnaires. Results: Patients exhibited a decrease in abduction and flexion force (−24.47% and −25.30%, respectively, p < 0.001) using the contralateral, uninjured arm as reference. Abduction force tended to be decreased in three- and four-part fractures. Patient satisfaction correlated negatively with the relatively reduced force of the affected arm. Varus-angulated humeral heads produced significantly lower abduction force output than valgus- or physiologic angulation (p = 0.014), whereas flexion force was unaffected (p = 0.468). The anatomical reduction had no influence on shoulder strength. Conclusions: Proximal humerus fractures may cause a significant reduction in shoulder function, both reported by patients and objectified by shoulder strength testing. Varus head angulation demonstrated the greatest loss of shoulder strength and should be avoided to ensure proper functioning. Further, strength testing seems a valuable outcome parameter for a thorough shoulder examination with easy obtainability.
Innovative Strategies in X-ray Crystallography for Exploring Structural Dynamics and Reaction Mechanisms in Metabolic Disorders
Authors: Grieco Alice , Quereda-Moraleda Isabel , Martin-Garcia Jose Manuel
Published: August 2024

Enzymes are crucial in metabolic processes, and their dysfunction can lead to severe metabolic disorders. Structural biology, particularly X-ray crystallography, has advanced our understanding of these diseases by providing 3D structures of pathological enzymes. However, traditional X-ray crystallography faces limitations, such as difficulties in obtaining suitable protein crystals and studying protein dynamics. X-ray free-electron lasers (XFELs) have revolutionized this field with their bright and brief X-ray pulses, providing high-resolution structures of radiation-sensitive and hard-to-crystallize proteins. XFELs also enable the study of protein dynamics through room temperature structures and time-resolved serial femtosecond crystallography, offering comprehensive insights into the molecular mechanisms of metabolic diseases. Understanding these dynamics is vital for developing effective therapies. This review highlights the contributions of protein dynamics studies using XFELs and synchrotrons to metabolic disorder research and their application in designing better therapies. It also discusses G protein-coupled receptors (GPCRs), which, though not enzymes, play key roles in regulating physiological systems and are implicated in many metabolic disorders.
Predictive Models for Aggregate Available Capacity Prediction in Vehicle-to-Grid Applications
Authors: Patanè Luca , Sapuppo Francesca , Napoli Giuseppe , Xibilia Maria Gabriella
Published: August 2024

The integration of vehicle-to-grid (V2G) technology into smart energy management systems represents a significant advancement in the field of energy suppliers for Industry 4.0. V2G systems enable a bidirectional flow of energy between electric vehicles and the power grid and can provide ancillary services to the grid, such as peak shaving, load balancing, and emergency power supply during power outages, grid faults, or periods of high demand. In this context, reliable prediction of the availability of V2G as an energy source in the grid is fundamental in order to optimize both grid stability and economic returns. This requires both an accurate modeling framework that includes the integration and pre-processing of readily accessible data and a prediction phase over different time horizons for the provision of different time-scale ancillary services. In this research, we propose and compare two data-driven predictive modeling approaches to demonstrate their suitability for dealing with quasi-periodic time series, including those dealing with mobility data, meteorological and calendrical information, and renewable energy generation. These approaches utilize publicly available vehicle tracking data within the floating car data paradigm, information about meteorological conditions, and fuzzy weekend and holiday information to predict the available aggregate capacity with high precision over different time horizons. Two data-driven predictive modeling approaches are then applied to the selected data, and the performance is compared. The first approach is Hankel dynamic mode decomposition with control (HDMDc), a linear state-space representation technique, and the second is long short-term memory (LSTM), a deep learning method based on recurrent nonlinear neural networks. In particular, HDMDc performs well on predictions up to a time horizon of 4 h, demonstrating its effectiveness in capturing global dynamics over an entire year of data, including weekends, holidays, and different meteorological conditions. This capability, along with its state-space representation, enables the extraction of relationships among exogenous inputs and target variables. Consequently, HDMDc is applicable to V2G integration in complex environments such as smart grids, which include various energy suppliers, renewable energy sources, buildings, and mobility data.
Manufacturer Encroachment on a Sustainable Supply Chain under Asymmetric Green Information
Authors: Hu Yufei , Du Xiaorong , Chen Lianghua
Published: August 2024

This paper investigates manufacturer encroachment on a sustainable supply chain, where the manufacturer holds exclusive information on product greenness and is responsible for both corporate social responsibility (CSR) and greening. The manufacturer and the retailer play a signaling game whereby CSR effort and wholesale price serve as joint green signals. Findings reveal that, firstly, encroachment induces higher CSR efforts from manufacturers. When customers exhibit a strong CSR preference, the resulting CSR increment leads to increased offline demand and drives up both wholesale and retail prices in a mutually beneficial manner. This phenomenon is referred to as the CSR effect, yielding a win-win encroachment. Secondly, when signaling product greenness to highly CSR-sensitive customers, the high-greenness manufacturer principally distorts her CSR effort downward to an extent unprofitable for the low-greenness manufacturer to mimic and subordinately distorts the wholesale price downward to counter CSR-induced demand decrement and mitigate CSR cost pass-through downstream. Finally, the win-win encroachment pattern is characterized by encroachment profit and signal expense sharing, with encroachment strengthening downward-distorted signaling while signaling weakens the CSR effect. These insights contribute valuable guidance for green manufacturers in CSR decision-making, which functions as a component of green signaling and facilitates transitioning to dual-channel sustainable supply chains.
Multi-Level Behavioral Mechanisms and Kinematic Modeling Research of Cellular Space Robot
Authors: Liu Xiaomeng , Gu Haiyu , Zhang Xiangyu , Duan Jianyu , Liu Zhaoxu , Li Zhichao , Wang Siyu , You Bindi
Published: August 2024

The cellular space robot (CSR) is a new type of self-reconfigurable robot. It can adapt the variety of on-orbit service tasks with large space spans through multi-level reconfiguration mechanisms. As the CSR has a large configuration space, kinematic solving becomes a key problem affecting on-orbit operation capability, and kinematic automatic solving research must be conducted. In order to solve this problem, firstly, the cellular space robot system capable of realizing multi-level self-reconfiguration is proposed for the demand of space on-orbit service, and the kinematic equations of modules are constructed by considering a single module function using screw theory. Secondly, the kinematics of the cellular space robot are encapsulated and divided into multiple levels, and the multilevel-assembly relationship-description method for robotic systems is proposed based on graph theory. On this basis, the pathway-solving algorithm is proposed to express the robot organization reachability information. Finally, the module–organ–robot multilevel kinematics solving algorithm is proposed in combination with screw theory. In order to verify the effectiveness of the algorithm in this paper, numerical simulation is used to compare with the proposed algorithm. The results show that compared with the traditional algorithm, the method in this paper only needs to update part of the assembly relations after organ migration, which simplifies the kinematic modeling operation and improves the efficiency of kinematic computation.
Fuzzy Resilient Control of DC Microgrids with Constant Power Loads Based on Markov Jump Models
Authors: Hu Wei , Shen Yu , Yang Fan , Chang Zhen , Zhao Shanglin
Published: August 2024

This paper addresses the fuzzy resilient control of DC microgrids with constant power loads. The DC microgrid is subject to abrupt parameter changes which are described by the Markov jump model. Due to the constant power loads, the DC microgrid exhibits nonlinear dynamics which are characterized by a T-S fuzzy model. According to the parallel distributed compensation principle, mode-dependent fuzzy resilient controllers are designed to stabilize the resultant T-S fuzzy Markov jump DC microgrid. The “resilient” means the controller could cope with the uncertainty caused by the inaccurate execution of the control laws. This uncertainty is governed by a Bernoulli distributed random variable and thus may not occur. Then, the mean square exponential stability is analyzed for the closed-loop system by using the mode-dependent Lyapunov function. Since the stability conditions are not convex, a design algorithm is further derived to calculate the fuzzy resilient controller gains. Finally, simulations are provided to test the effectiveness of the proposed results.
On Convoluted Forms of Multivariate Legendre-Hermite Polynomials with Algebraic Matrix Based Approach
Authors: Riyasat Mumtaz , Alali Amal S. , Wani Shahid Ahmad , Khan Subuhi
Published: August 2024

The main purpose of this article is to construct a new class of multivariate Legendre-Hermite-Apostol type Frobenius-Euler polynomials. A number of significant analytical characterizations of these polynomials using various generating function techniques are provided in a methodical manner. These enactments involve explicit relations comprising Hurwitz-Lerch zeta functions and λ-Stirling numbers of the second kind, recurrence relations, and summation formulae. The symmetry identities for these polynomials are established by connecting generalized integer power sums, double power sums and Hurwitz-Lerch zeta functions. In the end, these polynomials are also characterized Svia an algebraic matrix based approach.
Investigation of Properties in Magnesium Alloy Thin Plates after Die Casting Processes
Authors: Han Jun-Tae , Ryu Choong-Mo , Moon Seung-Jae
Published: August 2024

This study systematically analyzed the effect of design conditions on filling behavior and product characteristics when forming thin plates of magnesium alloy (AZ91D) of 0.5 mm or less using the die casting method. As a research method, a casting analysis simulation program was used to predict filling and solidification behavior under various process conditions. The molten metal injection temperature (610~670 °C), mold temperature (160~220 °C), and cooling water temperature (10~55 °C) were selected as key variables, and an analysis was performed for a total of five conditions. A simulation was conducted to analyze the charging speed distribution, location of oxides and bubbles, and solidification pattern. As a result of the study, the flow of molten metal in the low and high-speed sections of the plunger, uniformity of product thickness, and supply conditions of the molten metal were confirmed to be major factors. It is important to manage the molten metal injection temperature at an appropriate level to minimize product defects. Based on these conditions, a prototype was manufactured, the microstructure was observed, and a fine and uniform grain structure was observed in most areas. In mechanical property evaluation, superior physical properties were secured compared to existing bulk materials.
High-Cycle Fatigue Performance of Laser Powder Bed Fusion Ti-6Al-4V Alloy with Inherent Internal Defects: A Critical Literature Review
Authors: Li Zongchen , Affolter Christian
Published: August 2024

The inadequate fatigue performance of Laser Powder Bed Fusion (L-PBF) Ti-6Al-4V alloy, primarily due to intrinsic defects, poses a significant challenge for industrial applications. Internal defects often serve as initiation sites for fatigue cracks, significantly impacting the fatigue life of L-PBF Ti-6Al-4V components. Accurate evaluation of the role of internal defects in fatigue performance and quantitative analysis of influential parameters are crucial for guiding optimal L-PBF manufacturing design. This study aims to critically review recent notable contributions focusing on high-cycle fatigue (HCF) in these alloys, with many of the presented insights being easily transferred to other types of AM alloys. Efforts have been made to identify correlations between fatigue life at various stages and critical internal defects. Key aspects, including microstructure and post-processing treatments, and their effects on HCF have been thoroughly analyzed. The findings enhance the scientific understanding of fatigue performance of L-PBF Ti-6Al-4V alloy and open new avenues for future research.
The Legacy Effect of Mountain Pine Beetle Outbreaks on the Chemical and Anatomical Defences of Surviving Lodgepole Pine Trees
Authors: Baker Gigi , Zhao Shiyang , Klutsch Jennifer G. , Ishangulyyeva Guncha , Erbilgin Nadir
Published: August 2024

The recent mountain pine beetle outbreaks have caused widespread mortality among lodgepole pine trees in western North America, resulting in a reduced population of surviving trees. While previous studies have focused on the cascading impacts of these outbreaks on the physiology and growth of the surviving trees, there remains a need for a comprehensive study into the interactions among various physiological traits and the growth in post-outbreak stands. Specifically, the relationship between chemical (primarily terpenes) and anatomical (mainly resin ducts) defences, as well as the allocation of non-structural carbohydrates (NSCs) to support these defence modalities, is poorly understood. To address these gaps, we conducted a field survey of surviving lodgepole pine trees in post-mountain pine beetle outbreak stands in western Canada. Our retrospective analysis aimed at determining correlations between the post-outbreak concentrations of monoterpenes, diterpenes, and NSCs in the phloem and the historical resin duct characteristics and growth traits before and after the outbreak. We detected strong correlations between the post-outbreak concentrations of monoterpenes and historical resin duct characteristics, suggesting a possible link between these two defence modalities. Additionally, we found a positive relationship between the NSCs and the total concentrations of monoterpenes and diterpenes, suggesting that NSCs likely influence the production of these terpenes in lodgepole pine. Furthermore, historical tree growth patterns showed strong positive correlations with many individual monoterpenes and diterpenes. Interestingly, while surviving trees had enhanced anatomical defences after the outbreak, their growth patterns did not vary before and after the outbreak conditions. The complexity of these relationships emphasizes the dynamics of post-outbreak stand dynamics and resource allocations in lodgepole pine forests, highlighting the need for further research. These findings contribute to a broader understanding of conifer defences and their coordinated responses to forest insect outbreaks, with implications for forest management and conservation strategies.
Photonics-Based Multifunction System for Radar Signal Transmit-Receive Processing and Frequency Measurement
Authors: Yang Dengcai , Zhang Ya , Yang Feng , Yang Mei , Cao Yinhua
Published: August 2024

A novel photonic-assisted multifunctional radar system was proposed and experimentally investigated. This system can simultaneously achieve frequency-doubled linear frequency modulation (LFM) signal generation, de-chirp reception, self-interference cancellation, and frequency measurement in an integrated transmit-receive radar. First, a high-frequency and broadband LO signal was obtained with photonic frequency doubling, which improved the center frequency and bandwidth of the radar detection system. Then, photonic-assisted interference cancellation was used to reduce the impact of interference signals in radar de-chirp reception. Finally, the microwave frequency measurement was achieved by establishing a mapping relationship between the envelope response time of the intermediate frequency (IF) electrical filter and the microwave frequency to be tested. Both theoretical and experimental investigations were performed. The results showed that an LFM signal with a frequency range of 12–18 GHz was obtained with photonic frequency doubling. Photonic-assisted self-interference cancellation reduced the impact of interference signals in radar de-chirp reception by more than 12.1 dB for an LFM signal bandwidth of 6 GHz. In the frequency measurement module, the difference between the frequency to be tested, generated by the external signal source, and that calculated in the experiment is the measurement error, and a measurement resolution better than 14 MHz was achieved in the range of 12.14 GHz–18.14 GHz. The proposed system is suitable for miniaturized multifunctional radar signal processing systems with continuous operation of transmitting and receiving antennas in unmanned aerial vehicles (UAVs), automotive radar, relatively close spatial locations, and so on. In addition, it can simplify the system structure and reduce space occupation.
Textile Bandwidth-Enhanced Half-Mode Substrate-Integrated Cavity Antenna Based on Embroidered Shorting Vias
Authors: Liu Feng-Xue , Meng Fan-Yu , Chen Yu-Jia , Gao Zhou-Hao , Cui Jie , Zhang Le
Published: August 2024

A textile bandwidth-enhanced half-mode substrate-integrated cavity (HMSIC) antenna based on embroidered shorting vias is designed. Based on the simulated results of the basic HMSIC antenna, two embroidered hollow posts with square cross-sections are added as shorting vias at the intersections of the zero-E traces of the TM210HM and TM020HM modes to shift the TM010HM-mode band to merge with the bands of the higher-order modes for bandwidth enhancement. A prototype is practically fabricated based on computerized embroidery techniques. Measurement results show that the prototype is of an expanded −10 dB impedance band of 4.87~6.17 GHz (23.5% fractional bandwidth), which fully covers the 5 GHz wireless local area network (WLAN) band. The simulated radiation efficiency and maximum gain of the proposed antenna are above 97% and 7.6 dBi, respectively. Furthermore, simulations and measurements prove its robust frequency response characteristic in the proximity of the human tissues or in bending conditions, and the simulations of the specific absorption rate (SAR) prove its electromagnetic safety on the human body.
Enhancing 3D Printing Copper-PLA Composite Fabrication via Fused Deposition Modeling through Statistical Process Parameter Study
Authors: Moradi Mahmoud , Mehrabi Omid , Rasoul Fakhir A. , Mattie Anas Abid , Schaber Friedemann , Khandan Rasoul
Published: August 2024

The rapid advancement of additive manufacturing (AM) technologies has provided new avenues for creating three-dimensional (3D) parts with intricate geometries. Fused Deposition Modeling (FDM) is a prominent technology in this domain, involving the layer-by-layer fabrication of objects by extruding a filament comprising a blend of polymer and metal powder. This study focuses on the FDM process using a filament of Copper–Polylactic Acid (Cu-PLA) composite, which capitalizes on the advantageous properties of copper (high electrical and thermal conductivity, corrosion resistance) combined with the easily processable thermoplastic PLA material. The research delves into the impact of FDM process parameters, specifically, infill percentage (IP), infill pattern (P), and layer thickness (LT) on the maximum failure load (N), percentage of elongation at break, and weight of Cu-PLA composite filament-based parts. The study employs the response surface method (RSM) with Design-Expert V11 software. The selected parameters include infill percentage at five levels (10, 20, 30, 40, and 50%), fill patterns at five levels (Grid, Triangle, Tri-Hexagonal, Cubic-Subdivision, and Lines), and layer thickness at five levels (0.1, 0.2, 0.3, 0.4, and 0.5 mm). Also, the optimal factor values were obtained. The findings highlight that layer thickness and infill percentage significantly influence the weight of the samples, with an observed increase as these parameters are raised. Additionally, an increase in layer thickness and infill percentage corresponds to a higher maximum failure load in the specimens. The peak maximum failure load (230 N) is achieved at a 0.5 mm layer thickness and Tri-Hexagonal pattern. As the infill percentage changes from 10% to 50%, the percentage of elongation at break decreases. The maximum percentage of elongation at break is attained with a 20% infill percentage, 0.2 mm layer thickness, and 0.5 Cubic-Subdivision pattern. Using a multi-objective response optimization, the layer thickness of 0.152 mm, an infill percentage of 32.909%, and a Grid infill pattern was found to be the best configuration.
Microbial-Derived Carotenoids and Their Health Benefits
Authors: Sharma Chikanshi , Kamle Madhu , Kumar Pradeep
Published: August 2024

Natural carotenoids (CARs) such as β-carotene, astaxanthin, lutein, norbixin, bixin, capsanthin, lycopene, β-Apo-8-carotenal, canthaxanthin, β-apo-8-carotenal-ester, and zeaxanthin are being explored for possible applications in feed, food, cosmeceuticals, and nutraceuticals. Three primary areas of carotenoid research are emerging: (1) encapsulations for improved chemical and physical properties; (2) natural source carotenoid manufacturing; and (3) preclinical, epidemiological, and clinical studies of carotenoids’ potential health benefits. The recent advancements in research on the chemistry and antioxidant activity, marketing strategies, dietary sources, bioavailability, and bioaccessibility, extraction, dietary consumption, encapsulating techniques, and health advantages of carotenoids are all extensively discussed in this review. Carotenoids are pigments found naturally in most fruits and vegetables, algae, plants, and photosynthetic bacteria. Carotenoids cannot be synthesized by humans and must be consumed in the form of food or supplements. There are several roles for carotenoids in human health. Although individual carotenoids may function in different ways, their main action is to act as antioxidants. There are validated techniques for separating and purifying carotenoids, yet, industrial production requires the development of economically viable techniques for larger-scale implementation. Carotenoids have been shown to boost cognitive performance and cardiovascular health, as well as help prevent some types of cancer. Despite evidence for carotenoids’ health benefits, major population-based supplementation trials have yielded conflicting outcomes for several carotenoids. This review includes recent developments in carotenoid metabolism and nutritional and health advantages. It also offers an outlook on future directions in these areas.
A Haloarchaeal Transcriptional Regulator That Represses the Expression of CRISPR-Associated Genes
Authors: Turgeman-Grott Israela , Shalev Yarden , Shemesh Netta , Levy Rachel , Eini Inbar , Pasmanik-Chor Metsada , Gophna Uri
Published: August 2024

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas (CRISPR-associated proteins) systems provide acquired heritable protection to bacteria and archaea against selfish DNA elements, such as viruses. These systems must be tightly regulated because they can capture DNA fragments from foreign selfish elements, and also occasionally from self-chromosomes, resulting in autoimmunity. Most known species from the halophilic archaeal genus Haloferax contain type I-B CRISPR-Cas systems, and the strongest hotspot for self-spacer acquisition by H. mediterranei was a locus that contained a putative transposable element, as well as the gene HFX_2341, which was a very frequent target for self-targeting spacers. To test whether this gene is CRISPR-associated, we investigated it using bioinformatics, deletion, over-expression, and comparative transcriptomics. We show that HFX_2341 is a global transcriptional regulator that can repress diverse genes, since its deletion results in significantly higher expression of multiple genes, especially those involved in nutrient transport. When over-expressed, HFX_2341 strongly repressed the transcript production of all cas genes tested, both those involved in spacer acquisition (cas1, 2 and 4) and those required for destroying selfish genetic elements (cas3 and 5–8). Considering that HFX_2341 is highly conserved in haloarchaea, with homologs that are present in species that do not encode the CRISPR-Cas system, we conclude that it is a global regulator that is also involved in cas gene regulation, either directly or indirectly.
Mineralogical Characterization and Geochemical Signatures of Supergene Kaolinitic Clay Deposits: Insight of Ropp Complex Kaolins, Northcentral Nigeria
Authors: Yunusa Adamu , Hong Hanlie , Salim Atif , Amam Tarig , Liu Chen , Xu Yanxiao , Zuo Xiaochao , Li Zhaohui
Published: August 2024

This study presents the chemical and mineralogical composition of clay deposits and associated rock types within the Ropp Complex in order to assess the influence of parent lithology on the kaolinization, genesis, and utility of the deposit. Representative kaolin samples from E horizons of the weathering profiles and their bedrocks were collected from six sites in the Ropp Complex. Clay mineralogy was determined via the XRD technique, while a geochemical analysis was conducted using XRF, SEM coupled with EDS, and ICP-MS. The results showed that all kaolins dominantly contain kaolinite with a content of 77%–98% except for the AS1 kaolin with only minor kaolinite (20%) but mainly illite (65%). The notably lower crystallinity of kaolinite (HI value of 0.53–1.1) as well as its markedly small grain size is consistent with the formation of kaolinite from intensive chemical weathering of igneous rocks. The AS1 kaolin was probably formed from hydrothermal alteration in the burial stage due to the heating of groundwater by the late volcanism. Mobile trace elements (Sr, Ba, and Eu) exhibited a depletion trend, while immobile elements (Hf, Ta, Th) showed enrichment. The relatively more zirconium in kaolins implies the formation of low-temperature kaolinization. The notably high kaolinite content, accompanied by reasonable levels of Fe2O3 and TiO2, signifies a medium-grade quality. Furthermore, chondrite-normalized rare earth element (REE) patterns exhibit congruent trends in rocks and kaolin samples, indicating a relative enrichment in light rare earth elements (LREEs) alongside a discernible negative Eu anomaly. The abundant kaolinite and silicon–aluminum composition make the kaolins suitable for refractories, pharmaceutics, cosmetics, and supplementary cementitious material (SCM).
Characteristics and Control Factors of a High-Quality Deeply Buried Calcareous Sandstone Reservoir, the Fourth Member of the Upper Xujiahe Formation in the Western Sichuan Basin, China
Authors: Wu Dong , Yu Yu , Lin Liangbiao , Chen Hongde , Liu Sibing
Published: August 2024

A special type of sandstone in which carbonate rock fragments (CRFs) dominate the composition developed in the Upper Triassic Xujiahe Formation’s fourth member (Xu4) in the western Sichuan Basin, known as calcareous sandstone. Calcareous sandstones are widely distributed in the western Sichuan and is the main production target of tight sandstone gas in the Sichuan Basin. In this study, thin sections, porosity–permeability testing, scanning electron microscopy, and X-ray diffraction are applied to examine the characteristics and control factors for high-quality reservoirs in the calcareous sandstones, with a view to providing guidance for natural gas exploration and development in calcareous sandstones. The results show that the calcareous sandstone belongs to litharenite, with an average framework grain composition of 30% quartz, 1% feldspar, and 69% rock fragments, while the Xu4 sandstone has a high quartz content (average content of 71%). Primary intergranular pores are the main storage space, and the reservoir quality is quite poor. Under the influence of different parent rock properties of sandstones, there are obvious differences in the composition of framework grains between the calcareous sandstone and the ordinary Xu4 sandstone, which in turn affects the reservoir storage space, diagenesis, and reservoir quality. High-energy depositional conditions, low content of late cements, and the development of fractures are the main controlling factors for the formation of high-quality reservoirs in Xu 4 calcareous sandstones.
Molecularly Imprinted Microspheres in Active Compound Separation from Natural Product
Authors: Ahadi Husna Muharram , Fardhan Firghi Muhammad , Rahayu Driyanti , Pratiwi Rimadani , Hasanah Aliya Nur
Published: August 2024

Molecularly Imprinted Microspheres (MIMs) or Microsphere Molecularly Imprinted Polymers represent an innovative design for the selective extraction of active compounds from natural products, showcasing effectiveness and cost-efficiency. MIMs, crosslinked polymers with specific binding sites for template molecules, overcome irregularities observed in traditional Molecularly Imprinted Polymers (MIPs). Their adaptability to the shape and size of target molecules allows for the capture of compounds from complex mixtures. This review article delves into exploring the potential practical applications of MIMs, particularly in the extraction of active compounds from natural products. Additionally, it provides insights into the broader development of MIM technology for the purification of active compounds. The synthesis of MIMs encompasses various methods, including precipitation polymerization, suspension polymerization, Pickering emulsion polymerization, and Controlled/Living Radical Precipitation Polymerization. These methods enable the formation of MIPs with controlled particle sizes suitable for diverse analytical applications. Control over the template-to-monomer ratio, solvent type, reaction temperature, and polymerization time is crucial to ensure the successful synthesis of MIPs effective in isolating active compounds from natural products. MIMs have been utilized to isolate various active compounds from natural products, such as aristolochic acids from Aristolochia manshuriensis and flavonoids from Rhododendron species, among others. Based on the review, suspension polymerization deposition, which is one of the techniques used in creating MIPs, can be classified under the MIM method. This is due to its ability to produce polymers that are more homogeneous and exhibit better selectivity compared to traditional MIP techniques. Additionally, this method can achieve recovery rates ranging from 94.91% to 113.53% and purities between 86.3% and 122%. The suspension polymerization process is relatively straightforward, allowing for the effective control of viscosity and temperature. Moreover, it is cost-effective as it utilizes water as the solvent.
The Use of Beetroot Juice as an Impregnating Solution to Change Volatile Compounds, Physical Properties and Influence the Kinetics of the Celery Drying Process
Authors: Kręcisz Magdalena , Klemens Marta , Latański Aleks , Stępień Bogdan
Published: August 2024

The effect of different methods of drying celery root enriched with beet juice by vacuum impregnation (VI) was studied. The process of convection drying, vacuum drying and freeze drying was carried out. Compared to dried indigenous celery, dry impregnated tissue was characterized by lower values of dry matter, L* and b* color parameters, as well as higher values of water activity, density and a* color parameter. In addition, VI reduced the drying time. Forty Volatile Organic Compounds (VOCs) were found in celery, while fifty-one VOCs were found in the profile of celery with beetroot juice. The innovative method of vacuum impregnation made it possible to produce a new type of product with changed properties and a variable VOCs profile. The best fit of the drying process kinetics was achieved by using the logistic model. Increasing the temperature during convection drying resulted in shorter drying time, increased values of dry matter, reduced the water activity value and altered VOCs.
Influence of Bi3+ Doping on Electrochemical Properties of Ti/Sb-SnO2/PbO2 Electrode for Zinc Electrowinning
Authors: Wu Jia , Kang Xuanqi , Xu Shuangwen , Wei Zhen , Xu Shangyuan , Liu Kang , Feng Qing , Jia Bo , Wang Yunhai
Published: August 2024

Bi3+ doped Ti/Sb-SnO2/PbO2 electrode materials were fabricated by electrodeposition to improve their electrochemical performance in zinc electrowinning. The surface morphology, chemical composition, and hydrophilicity of the as-prepared electrodes were characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and contact angle. An electrochemical measurement and an accelerated lifetime experiment were also conducted to investigate the electrocatalytic performance and stability of the electrodes. The results show that the Bi3+ modification electrode has an important effect on the coating morphology, the crystal structure, the surface hydrophilicity, the electrocatalytic activity, and the stability. The electrode prepared from the solution containing 2 mmol·L−1 Bi(NO3)3 (marked as the Ti/Sb-SnO2/2Bi-PbO2 electrode) exhibits the best hydrophilicity performance (θ = 21.6°) and the longest service life (1196 h). During the electrochemical characterization analysis, the Ti/Sb-SnO2/2Bi-PbO2 electrode showed the highest oxygen evolution activity, which can be attributed to it having the highest electroactive surface (qT* = 21.20 C·cm−2) and the best charge-transfer efficiency. The DFT calculation demonstrated that the doping of Bi3+ leads to a decrease in the OER reaction barrier and an increase in the DOS of the electrode, which further enhances the catalytic activity and the conductivity of the electrode. Moreover, the simulated zinc electrowinning experiment demonstrated that the Ti/Sb-SnO2/2Bi-PbO2 electrode consumes less energy than other electrodes. Therefore, it is expected that the Bi3+ modified electrode will become a very promising electrode material for zinc electrowinning in the future.
Saikosaponin A Recovers Impaired Filaggrin Levels in Inflamed Skin by Downregulating the Expression of FRA1 and c-Jun
Authors: Ahn Sung Shin , Yeo Hyunjin , Jung Euitaek , Kim Tae Yoon , Han Junekyu , Lee Young Han , Shin Soon Young
Published: August 2024

Filaggrin (FLG) is an essential structural protein expressed in differentiated keratinocytes. Insufficient FLG expression contributes to the pathogenesis of chronic inflammatory skin diseases. Saikosaponin A (SSA), a bioactive oleanane-type triterpenoid, exerts anti-inflammatory activity. However, the effects of topically applied SSA on FLG expression in inflamed skin remain unclear. This study aimed to evaluate the biological activity of SSA in restoring reduced FLG expression. The effect of SSA on FLG expression in HaCaT cells was assessed through various biological methods, including reverse transcription PCR, quantitative real-time PCR, immunoblotting, and immunofluorescence staining. TNFα and IFNγ decreased FLG mRNA, cytoplasmic FLG protein levels, and FLG gene promoter–reporter activity compared to the control groups. However, the presence of SSA restored these effects. A series of FLG promoter–reporter constructs were generated to investigate the underlying mechanism of the effect of SSA on FLG expression. Mutation of the AP1-binding site (mtAP1) in the −343/+25 FLG promoter–reporter abrogated the decrease in reporter activities caused by TNFα + IFNγ, suggesting the importance of the AP1-binding site in reducing FLG expression. The SSA treatment restored FLG expression by inhibiting the expression and nuclear localization of FRA1 and c-Jun, components of AP1, triggered by TNFα + IFNγ stimulation. The ERK1/2 mitogen-activated protein kinase signaling pathway upregulates FRA1 and c-Jun expression, thereby reducing FLG levels. The SSA treatment inhibited ERK1/2 activation caused by TNFα + IFNγ stimulation and reduced the levels of FRA1 and c-Jun proteins in the nucleus, leading to a decrease in the binding of FRA1, c-Jun, p-STAT1, and HDAC1 to the AP1-binding site in the FLG promoter. The effect of SSA was evaluated in an animal study using a BALB/c mouse model, which induces human atopic-dermatitis-like skin lesions via the topical application of dinitrochlorobenzene. Topically applied SSA significantly reduced skin thickening, immune cell infiltration, and the expression of FRA1, c-Jun, and p-ERK1/2 compared to the vehicle-treated group. These results suggest that SSA can effectively recover impaired FLG levels in inflamed skin by preventing the formation of the repressor complex consisting of FRA1, c-Jun, HDAC1, and STAT1.
Design, Synthesis, and Anticancer Activity of Novel Enmein-Type Diterpenoid Derivatives Targeting the PI3K/Akt/mTOR Signaling Pathway
Authors: Wang Jiafeng , Wang Lu , Zhang Yingbo , Pan Siwen , Lin Yu , Wu Jiale , Bu Ming
Published: August 2024

The enmein-type diterpenoids are a class of anticancer ent-Kaurane diterpnoids that have received much attention in recent years. Herein, a novel 1,14-epoxy enmein-type diterpenoid 4, was reported in this project for the first time. A series of novel enmein-type diterpenoid derivatives were also synthesized and tested for anticancer activities. Among all the derivatives, compound 7h exhibited the most significant inhibitory effect against A549 cells (IC50 = 2.16 µM), being 11.03-folds better than its parental compound 4. Additionally, 7h exhibited relatively weak anti-proliferative activity (IC50 > 100 µM) against human normal L-02 cells, suggesting that it had excellent anti-proliferative selectivity for cancer cells. Mechanism studies suggested that 7h induced G0/G1 arrest and apoptosis in A549 cells by inhibiting the PI3K/AKT/mTOR pathway. This process was associated with elevated intracellular ROS levels and collapsed MMP. In summary, these data identified 7h as a promising lead compound that warrants further investigation of its anticancer properties.
Bright Yellow Luminescence from Mn2+-Doped Metastable Zinc Silicate Nanophosphor with Facile Preparation and Its Practical Application
Authors: Afandi Mohammad M. , Byeon Sanghun , Kang Taewook , Kang Hyeonwoo , Kim Jongsu
Published: August 2024

Mn2+-doped β-Zn2SiO4, a metastable phase of zinc silicate, is widely acknowledged for the uncertainties linked to its crystal structure and challenging synthesis process along with its distinctive yellowish luminescence. In this study, a vivid yellow luminescence originating from Mn2+-doped metastable zinc silicate (BZSM) nanophosphor is suggested, achieved through a straightforward single-step annealing process. The reliable production of this phosphor necessitates substantial doping, surplus SiO2, a brief annealing duration, and prompt cooling. The verification of the phase is demonstrated based on its optical and crystallographic characteristics. Moreover, the effective utilization of excimer lamps in practical scenarios is effectively demonstrated as a result of the vacuum ultraviolet excitation property of BZSM nanophosphor. This outcome paves the way for additional deployment of metastable zinc silicate in various fields, consequently generating novel prospects for future advancements.
Effects of Scutellaria baicalensis Extract-Induced Exosomes on the Periodontal Stem Cells and Immune Cells under Fine Dust
Authors: Yun Mihae , Kim Boyong
Published: August 2024

In adverse environments, fine dust is linked to a variety of health disorders, including cancers, cardiovascular, neurological, renal, reproductive, motor, systemic, and respiratory diseases. Although PM10 is associated with oral inflammation and cancer, there is limited research on biomaterials that prevent damage caused by fine dust. In this study, we evaluated the effects of biomaterials using microRNA profiling, flow cytometry, conventional PCR, immunocytochemistry, Alizarin O staining, and ELISA. Compared to SBE (Scutellaria baicalensis extract), the preventive effectiveness of SBEIEs (SBE-induced exosomes) against fine dust was approximately two times higher. Furthermore, SBEIEs promoted cellular differentiation of periodontal ligament stem cells (PDLSCs) into osteoblasts, periodontal ligament cells (PDLCs), and pulp progenitor cells (PPCs), enhancing immune modulation for oral health against fine dust. In terms of immune modulation, SBEIEs activated the secretion of cytokines such as IL-10, LL-37, and TGF-β in T cells, B cells, and macrophages, while attenuating the secretion of MCP-1 in macrophages. MicroRNA profiling revealed that significantly modulated miRNAs in SBEIEs influenced four biochemical categories: apoptosis, cellular differentiation, immune activation, and anti-inflammation. These findings suggest that SBEIEs are an optimal biomaterial for developing oral health care products. Additionally, this study proposes functional microRNA candidates for the development of pharmaceutical liposomes.
Resveratrol: A Multifaceted Guardian against Anxiety and Stress Disorders—An Overview of Experimental Evidence
Authors: Tseilikman Vadim E. , Tseilikman Olga B. , Yegorov Oleg N. , Brichagina Alina A. , Karpenko Marina N. , Tseilikman David V. , Shatilov Vladislav A. , Zhukov Maxim S. , Novak Jurica
Published: August 2024

The medicinal properties of resveratrol have garnered increasing attention from researchers. Extensive data have been accumulated on its use in treating cardiovascular diseases, immune system disorders, cancer, neurological diseases, and behavioral disorders. The protective mechanisms of resveratrol, particularly in anxiety-related stress disorders, have been well documented. However, less attention has been given to the side effects of resveratrol. This review explores not only the mechanisms underlying the anxiolytic effects of resveratrol but also the mechanisms that may lead to increased anxiety following resveratrol treatment. Understanding these mechanisms is crucial for enhancing the efficacy of resveratrol in managing anxiety disorders associated with stress and PTSD.
Cordycepin Ameliorates High Fat Diet-Induced Obesity by Modulating Endogenous Metabolism and Gut Microbiota Dysbiosis
Authors: Fu Yifeng , Wang Qiangfeng , Tang Zihan , Liu Gang , Guan Guiping , Lyu Jin
Published: August 2024

Background: Numerous metabolic illnesses have obesity as a risk factor. The composition of the gut microbiota and endogenous metabolism are important factors in the onset and progression of obesity. Recent research indicates that cordycepin (CRD), derived from fungi, exhibits anti-inflammatory and antioxidant properties, showing potential in combating obesity. However, further investigation is required to delineate its precise impacts on endogenous metabolism and gut microbiota. Methods: In this work, male C57BL/6J mice were used as models of obesity caused by a high-fat diet (HFD) and given CRD. Mice’s colon, liver, and adipose tissues were stained with H&E. Serum metabolome analysis and 16S rRNA sequencing elucidated the effects of CRD on HFD-induced obese mice and identified potential mediators for its anti-obesity effects. Results: CRD intervention alleviated HFD-induced intestinal inflammation, improved blood glucose levels, and reduced fat accumulation. Furthermore, CRD supplementation demonstrated the ability to modulate endogenous metabolic disorders by regulating the levels of key metabolites, including DL-2-aminooctanoic acid, inositol, and 6-deoxyfagomine. CRD influenced the abundance of important microbiota such as Parasutterella, Alloprevotella, Prevotellaceae_NK3B31_group, Alistipes, unclassified_Clostridia_vadinBB60_group, and unclassified_Muribaculaceae, ultimately leading to the modulation of endogenous metabolism and the amelioration of gut microbiota disorders. Conclusions: According to our research, CRD therapies show promise in regulating fat accumulation and stabilizing blood glucose levels. Furthermore, through the modulation of gut microbiota composition and key metabolites, CRD interventions have the dual capacity to prevent and ameliorate obesity.