bluuebunny/crossref_metadata_2025 huggingface dataset:
An Efficient Multi-Lane SpaceFibre Core for Spacecraft Data-Handling Networks
Authors: Zheng Jingya , An Junshe , Jiang Yuanyuan
Published: April 2022

As one of the key technologies in spacecraft data-handling networks mounting high-resolution payloads, the multi-lane native SpaceFibre standard is designed to meet the demands for the high-speed and reliable interconnections between satellite payloads. A few studies are about the implementations of a multi-lane SpaceFibre core. Even though they exhibit good performance, several limitations still, more or less, exist. We detail an efficient multi-lane SpaceFibre (E-ML-SpFi) core for high-rate and fault-tolerant data communication. The E-ML-SpFi core exploits a hierarchical method to decouple the complex control logic of the retransmission mechanism. The core employs a modified word re-ordering block to map an extended set of words on a real-time variable number of data-sending lanes. Meanwhile, the core introduces the architecture of a fast alignment subsystem to accelerate the alignment process. A hardware implementation on XC7Z100 FPGA shows that the E-ML-SpFi core has complete functionality stipulated by the standard and requires limited resources, with 6290 (2.26%) LUTs and 8252 (1.49%) FFs. The maximum operating frequency of the core is 200 MHz. Moreover, the recovery time is reduced up to 30.9% compared with previous work. Therefore, the core provides a valid solution for future interconnection networks onboard spacecraft.
Intelligent Reflecting Surface Assisted Localization: Opportunities and Challenges
Authors: Sur Samarendra Nath , Singh Arun Kumar , Kandar Debdatta , Silva Adão , Nguyen Nhan Duc
Published: April 2022

Future generation communication systems are aiming to provide a tremendous high data rate with low-latency high reliable and three-dimensional coverage. To achieve such a challenging goal, it is required to have very precise location information related to the mobile terminal. The advancement of signal processing techniques and communication technologies enables the path for improving localization performance. Recently, intelligent reflecting surface (IRS) has been widely considered as the key element for the future generation of wireless communication. Over the past few years, the performance of IRS-assisted networks is extensively investigated from the point of view of communication purposes and its improvement. However, by virtue of its potential, IRS finds its application for wireless localization. In this paper, we discuss and summarize the works that have already been carried out targeting localization performance improvement. In addition, we figured out the associated challenges and the opportunities to scale up the localization accuracy. Particularly in this paper, the authors have discussed the challenging issues such as channel modeling, channel estimation, system architecture, hardware impairment, IRS deployment strategies, phase optimization, mobility management, and near-field environments. Although these challenges are associated with opportunities to make the IRS-assisted system more effective and efficient.
Blockchain Applicability for the Internet of Things: Performance and Scalability Challenges and Solutions
Authors: Rahman Ziaur , Yi Xun , Mehedi Sk. Tanzir , Islam Rafiqul , Kelarev Andrei
Published: April 2022

Blockchain has recently drawn wide attention in the research community. Since its emergence, the world has seen the expansion of this new technology, which was initially developed as a digital currency more than a decade ago. A self-administering ledger that ensures extensive data immutability over a peer-to-peer network has made it attractive for cybersecurity applications, including sensor-enabled Internet of Things (IoT) systems. Brand new challenges and questions now demand solutions, as IoT devices are now online in a distributed fashion to simplify our everyday lives. Motivated by those challenges, the work here has detailed issues from which an IoT infrastructure can suffer if the wrong blockchain technology is chosen. Unlike a typical review, this paper focuses on security challenges of the blockchain-IoT ecosystem through critical findings and applicable use cases. The contribution directs Blockchain architects, designers, and researchers in the domain to select an unblemished combination of Blockchain-powered IoT applications. In addition, the paper provides insight into the state-of-the-art Blockchain platforms, namely Ethereum, Hyperledger, and IOTA, to exhibit their respective challenges, their constraints, and their prospects in terms of performance and scalability.
The Application of Machine Learning Methods to Predict the Power Output of Internal Combustion Engines
Authors: Yang Ruomiao , Xie Tianfang , Liu Zhentao
Published: April 2022

The indicated mean effective pressure (IMEP) is a key parameter for measuring the power output of an internal combustion engine (ICE). This indicator can be used to locate the high efficiency regions of engines. Therefore, it makes sense to predict the IMEP based on the machine learning (ML) approaches. However, different ML models are applicable to different scenarios, so it is important to choose the right model for prediction. The objective of this paper was to compare three ML models’ (ANN, SVR, RF) predictive performance in forecasting IMEP indicator with the input parameters spark timing (ST), speed and load. A validated one-dimensional (1D) computational fluid dynamics (CFD) model was employed to provide 756 sets of data for the training, validation, and testing of the model. The results indicated that the random forest (RF) model had the worst prediction performance, and support vector regression (SVR) had a slightly better prediction performance than the artificial neural network (ANN), at least for the investigations in this study. Overall, the ANN and SVR models showed good predictive performance for IMEP, as the coefficient of determination (R2) was close to unity, and the root mean squared error (RMSE) was close to zero. Whereas the overall prediction results of the RF model are acceptable, the RF model does not learn well for some internal engine laws.
Comparison of Machine Learning Methods Applied on Multi-Source Medium-Resolution Satellite Images for Chinese Pine (Pinus tabulaeformis) Extraction on Google Earth Engine
Authors: Liu Lizhi , Guo Ying , Li Yu , Zhang Qiuliang , Li Zengyuan , Chen Erxue , Yang Lin , Mu Xiyun
Published: April 2022

Chinese pine has tremendous applications in many fields. Mapping the distribution of Chinese pine is of great importance for government decision-making and forest management. In order to extract Chinese pine on a large scale, efficient algorithms and open remote-sensing datasets are needed. It is widely believed that machine learning algorithms and medium-resolution remote-sensing datasets can work well for this purpose. Unfortunately, their performance for Chinese pine extraction has remained unclear until now. Therefore, this study aims to explore the ability of the different machine learning algorithms and open remote-sensing datasets for Chinese pine extraction over large areas on Google Earth Engine (GEE). So, based on the combination of three typical machine learning algorithms, namely deep neural network (DNN), support vector machine (SVM), random forest (RF), and three open medium-resolution remote-sensing datasets, namely Sentinel-2, Gaofen-1, and Landsat-8 OLI, 27 models are constructed and GEE, with its powerful computing ability, is used. The main findings are as follows: (1) DNN has the highest accuracy for Chinese pine extraction, followed by SVM and RF; DNN is more sensitive to spatial geometric information, while SVM and RF algorithms are more sensitive to spectral information. (2) Spectral indexes are helpful for improving the extraction accuracy of Chinese pine. The extraction accuracy by using Gaofen-1 dataset increases 7.6% after adding spectral indexes, while the accuracies by using Sentinel-2 and Landsat-8 datasets increase 1.8% and 1.9% after adding spectral indexes, respectively. (3) The extraction accuracy by using DNN and Sentinel-2 dataset with spectral indexes is the highest, with an overall accuracy of 94.4%. (4) The area of Chinese pine is 153.73 km2, accounting for 5.06% of the administrative area of Karaqin Banner, and it is convenient to extract Chinese pine on a large scale by using GEE.
Assessment of Metal Intake by Selected Food Supplements Based on Beehive Products
Authors: Vujić Mario , Lušić Dražen , Bošnir Jasna , Pezo Lato L. , Kuharić Željka , Lasić Dario , Šabarić Jasenka , Barušić Lidija , Lušić Darija Vukić
Published: April 2022

The aim of this study was to determine the quantity of particular toxic metals (Pb, Cd, As, Hg) and micronutrients (Cr, Fe, Co, Ni, Cu, Zn, Se) in the recommended daily dose of 51 food supplements based on beehive products. Samples taken from the Croatian market were submitted for the identification/quantification of studied metals and micronutrients. It was carried out by means of inductively coupled plasma mass spectrometry (ICP-MS). Eleven samples (21.57%) showed an increased concentration of total arsenic, three samples (5.88%) contained an increased concentration of total iron, and eight samples (15.68%) had an increased concentration of total nickel. Three samples (5.88%) contained an increased concentration of zinc, while one sample (1.96%) contained an increased concentration of selenium. Increased levels of certain toxic metals and micronutrients do not pose a danger to human health because the amount identified was less than what can cause toxic effects in humans. All other analysed metals and micronutrients fell within the defined literature values. Despite certain increases in particular parameters, all samples met the established toxicity criteria. This study evidenced their safety if consumed in the recommended daily dose.
Physical Properties of Extrudates with Fibrous Structures Made of Faba Bean Protein Ingredients Using High Moisture Extrusion
Authors: Kantanen Katja , Oksanen Anni , Edelmann Minnamari , Suhonen Heikki , Sontag-Strohm Tuula , Piironen Vieno , Ramos Diaz Jose Martin , Jouppila Kirsi
Published: April 2022

Faba bean is a potential ingredient due to its high protein yield and its possible cultivation in colder climate regions. In this study, meat analogues made from faba bean protein isolate (FPI) and concentrate (FPC) blends were produced using high moisture extrusion. The aim of this study was to investigate the effect of the FPI content (FPIc), feed water content (FWC), and temperature of the long cooling die (LT) during extrusion on the mechanical and physicochemical properties as well as on the structure of the meat analogues. Increased FPIc resulted in higher values in hardness, gumminess, chewiness, and cutting strengths as well as in darker colour and decreased water absorption capacity. The effect of increased FWC on these properties was weaker and the opposite. Images from microtomography revealed that higher FPIc led to a less organised fibrous structure. In conclusion, fibrous structures can be achieved by utilising a mixture of faba bean protein ingredients, and a higher FPC content seemed to promote fibre formation in the meat analogue.
Effect of Durum Wheat Oil on the Physico-Chemical and Sensory Features of Biscuits
Authors: Vurro Francesca , Greco Miani Marcello , Summo Carmine , Caponio Francesco , Pasqualone Antonella
Published: April 2022

Lipids play an important role in defining the overall quality of biscuits, particularly in terms of resistance to oxidation, as well as for their influence on textural and sensorial properties. The aim of this work was to investigate the effects of durum wheat oil on the physico-chemical and sensory features of biscuits. Control biscuits (C) prepared with the commonly used sunflower oil were compared with samples prepared with durum wheat oil at 50% (D50) and 100% replacement levels (D100). The reformulated biscuits were very rich in tocols, especially tocotrienols (982.9, 635.2, and 64.1 mg/kg on lipid fraction weight in D100, D50, and C, respectively). The higher content of antioxidants extended the resistance to the oxidation of biscuits (induction time = 53.61, 70.87, and 79.92 h in C, D50, and D100, respectively). D100 showed the lowest amounts of triacylglycerol oligopolymers and oxidized triacylglycerols, and the lowest amounts of the volatile markers of lipid oxidation (hexanal and nonanal). The use of durum wheat oil did not affect the sensorial and textural properties, compared to C. This study suggests that durum wheat oil could be effectively used in biscuit-making to decrease the oxidative phenomena and increase the bioactives of the end-products.
Explaining Unsaturated Fatty Acids (UFAs), Especially Polyunsaturated Fatty Acid (PUFA) Content in Subcutaneous Fat of Yaks of Different Sex by Differential Proteome Analysis
Authors: Xiong Lin , Pei Jie , Wu Xiaoyun , Bao Pengjia , Guo Xian , Yan Ping
Published: April 2022

Residents on the Tibetan Plateau intake a lot of yak subcutaneous fat by diet. Modern healthy diet ideas demand higher unsaturated fatty acids (UFAs), especially polyunsaturated fatty acid (PUFA) content in meat. Here, the gas chromatography (GC) and tandem mass tag (TMT) proteomic approaches were applied to explore the relationship between the proteomic differences and UFA and PUFA content in the subcutaneous fat of yaks with different sex. Compared with male yaks (MYs), the absolute contents of UFAs, monounsaturated fatty acids (MUFAs) and PUFAs in the subcutaneous fat of female yaks (FYs) were all higher (p < 0.01); the relative content of MUFAs and PUFAs in MY subcutaneous fat was higher, and the value of PUFAs/SFAs was above 0.4, so the MY subcutaneous fat is more healthy for consumers. Further studies showed the transcriptional regulation by peroxisome proliferator-activated receptor delta (PPARD) played a key role in the regulation of UFAs, especially PUFA content in yaks of different sex. In FY subcutaneous fat, the higher abundance of the downstream effector proteins in PPAR signal, including acyl-CoA desaturase (SCD), elongation of very-long-chain fatty acids protein 6 (ELOVL6), lipoprotein lipase (LPL), fatty acid-binding protein (FABP1), very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3 (HACD3), long-chain fatty acid CoA ligase 5 (ACSL5) and acyl-CoA-binding protein 2 (ACBP2), promoted the UFAs’ transport and synthesis. The final result was the higher absolute content of c9-C14:1, c9-C18:1, c9,c12-C18:2n-6, c9, c12, c15-C18:3n-3, c5, c8, c11, c14, c17-C20:5n-3, c4, c7, c10, c13, -c16, c19-C22:6n-3, UFAs, MUFAs and PUFAs in FY subcutaneous fat. Further, LPL, FABP1, HACD3, ACSL1 and ACBP2 were the potential biomarkers for PUFA contents in yak subcutaneous fat. This study provides new insights into the molecular mechanisms associated with UFA contents in yak subcutaneous fat.
A More Comprehensive Way to Analyze Foam Stability for EPB Tunnelling—Introduction of a Mathematical Characterization
Authors: Carigi Andrea , Todaro Carmine , Martinelli Daniele , Peila Daniele
Published: April 2022

In the tunnelling industry, a large share of the market is occupied by EPB (Earth Pressure Balance) machines. To operate this kind of machine, a radical change in the rheological behaviour of the excavated soil must be performed, and this is achieved by adding water, foam, and, eventually, polymers. The stability of the foam is assessed through a half-life test. The main limitation of this test is that only one value is used in the characterization of the foam degradation process, which is insufficient to describe the whole evolution of the phenomenon. The results of more than 270 tests were modelled through a five-parameter mathematical formulation that suited the experimental data. The results show that the influence of concentration on the stability of the foam is not always present and that the flow rate used during production bears an influence on the characteristics of the foam.
UVA and UVB Radiation as Innovative Tools to Biofortify Horticultural Crops with Nutraceuticals
Authors: Jacobo-Velázquez Daniel A. , Moreira-Rodríguez Melissa , Benavides Jorge
Published: April 2022

The consumption of fruits and vegetables is related to the prevention and treatment of chronic–degenerative diseases due to the presence of secondary metabolites with pharmaceutical activity. Most of these secondary metabolites, also known as nutraceuticals, are present in low concentrations in the plant tissue. Therefore, to improve the health benefits of horticultural crops, it is necessary to increase their nutraceutical content before reaching consumers. Applying ultraviolet radiation (UVR) to fruits and vegetables has been a simple and effective technology to biofortify plant tissue with secondary metabolites. This review article describes the physiological and molecular basis of stress response in plants. Likewise, current literature on the mechanisms and effects of UVA and UVB radiation on the accumulation of different bioactive phytochemicals are reviewed. The literature shows that UVR is an effective tool to biofortify horticultural crops to enhance their nutraceutical content.
In Vitro Germination and Propagation of Dyckia brevifolia, An Ornamental and Endangered Bromeliad
Authors: Bertsouklis Konstantinos , Panagaki Konstantina-Panagiota
Published: April 2022

Dyckia brevifolia is an endangered plant used for ornamental purposes. As no references to the in vitro propagation of the species exist, the present study aims at investigating the possibility of an efficient micropropagation protocol. Seeds collected from mother plants were germinated at high percentages (84–86%) at a range of 15–25 °C, without any pre-treatment, and demonstrated their highest germination speed index (191.51) at 25 °C. In vitro-grown seedlings were used as the starting material for micropropagation on solid, or liquid, MS medium, supplemented with a variety of concentrations of cytokinins (BA, KIN or 2IP). Shoots and leaves were used as starting explants. Liquid media supplemented with BA or 2IP at 1.0 mg L−1 led to high multiplication rate and 2.7, or 2.3, lateral shoots were regenerated while on 2IP a high percentage (77.5%) of rooting occurred at the same time. Rooted microshoots were acclimatised ex vitro at 100% and acclimatised plants were transplanted in pots where they grew with a survival rate of 100% after two months. The in vitro propagation protocol presented in this study could enhance the large-scale propagation use of D. brevifolia as an ornamental plant and, simultaneously, contribute to the ex-situ conservation of the species.
Factors Associated with Mental Health Outcomes in Hospital Workers during the COVID-19 Pandemic: A Mixed-Methods Study
Authors: Huarcaya-Victoria Jeff , Villarreal-Rao Beltran , Luna Matilde , Rojas-Mendoza Wendoline , Alarcon-Ruiz Christoper A. , Villarreal-Zegarra David , Vilela-Estrada Ana L. , Ramírez Samantha
Published: April 2022

Background: We investigated some of the factors associated with depression, perceived stress, and anxiety in clinical and nonclinical healthcare workers of two hospitals. Methods: A mixed-methods approach was used. The sample included clinical (physicians, nurses, and others) and nonclinical (security and cleaning staff) healthcare workers of two tertiary hospitals in Peru. Participants completed an online self-survey. In the qualitative analysis, data were subjected to thematic analysis. Results: We analyzed data from 613 participants, of which 8.6%, 9.0%, and 78.2% had moderate-to-severe anxiety, depression, and perceived stress, respectively. Having a previous mental health problem, being concerned about losing one’s job, having at least two COVID-19 symptoms in the preceding two weeks, and being afraid of infecting family members increased the prevalence of experiencing moderate-to-severe depression and anxiety. The qualitative analysis allowed us to identify five recurring factors that caused a negative impact on workers’ lives during the pandemic: emotional distress linked to hospital experiences of suffering and death, modification of routines, fear of COVID-19, exacerbation of mental disorders, and physical problems associated with emotional distress. Conclusions: Clinical and nonclinical healthcare workers in Peru have experienced depression, anxiety, and stress during the COVID-19 pandemic. Future research and interventions are necessary to improve psychological support for hospital workers.
Simulated Forest Immersion Therapy: Methods Development
Authors: Ross Amy Miner , Jones Reo Jane Francesca
Published: April 2022

Shinrin-yoku, forest bathing, may provide relief from chronic and breakthrough pain in patients with axial spondyloarthritis and improve immune function through increasing NK cell numbers and activity and their downstream effectors, perforin and granulysin, after chemo- or radiation therapy in breast and prostate cancer patients. The aim of this paper is to describe the study protocol for a simulated forest immersion therapy using virtual reality and atomized phytoncides, volatile organic compounds found in forested areas designed to effect positive change for these two patient populations. The setting, including the room set up and samples with inclusion/exclusion specific to this type of intervention, is outlined. Measures and calibration procedures pertinent to determining the feasibility of simulated forest immersion therapy are presented and include: ambient and surface room temperatures and relative humidity in real time, ambient ultrafine particulate matter, ambient droplet measurement that coincides with volatile organic compounds, specific phytoncides, and virtual reality and atomization of phytoncide set up. Particular lessons learned while training and setting up the equipment are presented. Simulated forest immersion therapy is possible with attention to detail during this early phase when development of methods, equipment testing, and feasibility in deploying the intervention become operational. The expected outcome of the development of the methods for this study is the creation of a standardized approach to simulating forest therapy in a controlled laboratory space.
Fourteen Main Obstacles on the Journey to Post-Traumatic Growth as Experienced by Female Survivors of Intimate Partner Violence: “It Was All So Confusing”
Authors: Bryngeirsdottir Hulda S. , Halldorsdottir Sigridur
Published: April 2022

In this study, we identified 14 obstacles experienced by female survivors of intimate partner violence who had, nonetheless, reached post-traumatic growth (PTG), which is a positive psychological change by a person following serious difficulties or traumatic events. Intimate partner violence (IPV) is such a trauma. The purpose of this study was to analyze the obstacles to PTG as experienced by women who have succeeded in reaching PTG following traumatic IPV. Participants were twenty-two women aged 23–56 who self-reported their PTG according to the working definition used. The participants reported feelings of diminished self-worth that had negatively influenced their lives and how these negative feelings delayed their PTG. The overriding theme of the study was “It was all so confusing”, which expressed the essence of the participants’ feelings when describing the obstacles they encountered on their journey to PTG. Most of those obstacles were intrapersonal, i.e., negative personal feelings and negative perspectives towards themselves. Other obstacles reported by participants were physical and psychological health problems, challenging personal circumstances, and the perpetrator, as well as laws, regulations, and institutional social systems. This study reveals the broad range of obstacles encountered by women on their journey to PTG following IPV, emphasizing the necessity of an interdisciplinary approach when holistically considering their situation and supporting them on their journey towards PTG.
Social Media Use in E-Learning amid COVID 19 Pandemic: Indian Students’ Perspective
Authors: Sobaih Abu Elnasr E. , Palla Ishfaq Ahmad , Baquee Abdul
Published: April 2022

As a result of the COVID-19 epidemic, most educational institutions shifted to online education. Students and faculty members in many public institutions, particularly those in developing countries, are hampered by the absence of formal online learning management systems. Responding to COVID-19, many institutions in developing countries adopted social media sites to maintain e-learning and sustain education process. The distinction between online and real-world communities is becoming increasingly narrow, especially among the younger generations who have grown up with social media at their fingertips. This research explores perspectives of higher education students in India regarding the use of social media for e-learning amid the COVID-19 pandemic. For this purpose, an online questionnaire was directed to a sample of higher education students in India via a personal network. The results showed that students were more satisfied with their use of social media because of their perceptions of its ease of use and usefulness. The majority of the students are active on social media for 1–2 h daily (p < 0.01). YouTube was the platform of choice among all the respondents (n = 154; 36%). The results confirmed that students feel that social media websites have a significant positive impact on their overall academic performance (p < 0.01). Novel methods of teaching and learning are constantly being sought out by educators. The present moment is an opportunity to examine and analyze the theoretical benefits of social media technologies and consider their relative advantages for education through the use of technology’s ability to enhance student learning.
SARS-CoV-2 Circulation in the School Setting: A Systematic Review and Meta-Analysis
Authors: Caini Saverio , Martinoli Chiara , La Vecchia Carlo , Raimondi Sara , Bellerba Federica , D’Ecclesiis Oriana , Sasso Clementina , Basso Alessandra , Cammarata Giulio , Gandini Sara
Published: April 2022

The contribution of children to viral spread in schools is still debated. We conducted a systematic review and meta-analysis of studies to investigate SARS-CoV-2 transmission in the school setting. Literature searches on 15 May 2021 yielded a total of 1088 publications, including screening, contact tracing, and seroprevalence studies. MOOSE guidelines were followed, and data were analyzed using random-effects models. From screening studies involving more than 120,000 subjects, we estimated 0.31% (95% confidence interval (CI) 0.05–0.81) SARS-CoV-2 point prevalence in schools. Contact tracing studies, involving a total of 112,622 contacts of children and adults, showed that onward viral transmission was limited (2.54%, 95% CI 0.76–5.31). Young index cases were found to be 74% significantly less likely than adults to favor viral spread (odds ratio (OR) 0.26, 95% CI 0.11–0.63) and less susceptible to infection (OR 0.60; 95% CI 0.25–1.47). Lastly, from seroprevalence studies, with a total of 17,879 subjects involved, we estimated that children were 43% significantly less likely than adults to test positive for antibodies (OR 0.57, 95% CI 0.49–0.68). These findings may not applied to the Omicron phase, we further planned a randomized controlled trial to verify these results.
Towards a Core Set of Landscape Metrics of Urban Land Use in Wuhan, China
Authors: Shao Shiwei , Yu Mengting , Huang Yimin , Wang Yiheng , Tian Jing , Ren Chang
Published: April 2022

In this study, we investigate the urban landscape patterns in Wuhan, China based on the land use data in the vector format. Using the approach of landscape metric analysis, we calculate forty-four vector-based landscape metrics and then reduce redundant ones through a combination of Spearman correlation analysis and factor analysis, in order to extract a core set of characterizing landscape metrics. We find that the urban landscape can be depicted by six factors including the overall shape and diversity, mean proximity, overall area variation, fragmentation variation, elongation variation, and mean shape complexity. After analyzing typical patterns indicated by the core metrics and the spatial distribution of land use patterns, we compare our findings with other studies and discuss how the core metrics coincide and differ.
NGF Modulates Cholesterol Metabolism and Stimulates ApoE Secretion in Glial Cells Conferring Neuroprotection against Oxidative Stress
Authors: Colardo Mayra , Petraroia Michele , Lerza Letizia , Pensabene Daniele , Martella Noemi , Pallottini Valentina , Segatto Marco
Published: April 2022

Cholesterol plays a crucial role in the brain, where its metabolism is particularly regulated by astrocytic activity. Indeed, adult neurons suppress their own cholesterol biosynthesis and import this sterol through ApoE-rich particles secreted from astrocytes. Recent evidence suggests that nerve growth factor (NGF) may exert neurotrophic activity by influencing cell metabolism. Nevertheless, the effect of NGF on glial cholesterol homeostasis has still not been elucidated. Thus, the aim of this project is to assess whether NGF could influence cholesterol metabolism in glial cells. To reach this objective, the U373 astrocyte-derived cell line was used as an experimental model. Immunoblot and ELISA analysis showed that proteins and enzymes belonging to the cholesterol metabolism network were increased upon NGF treatment in glial cells. Furthermore, NGF significantly increased ApoE secretion and the amount of extracellular cholesterol in the culture medium. Co-culture and U373-conditioned medium experiments demonstrated that NGF treatment efficiently counteracted rotenone-mediated cytotoxicity in N1E-115 neuronal cells. Conversely, neuroprotection mediated by NGF treatment was suppressed when N1E-115 were co-cultured with ApoE-silenced U373 cells. Taken together, these data suggest that NGF controls cholesterol homeostasis in glial cells. More importantly, NGF exerts neuroprotection against oxidative stress, which is likely associated with the induction of glial ApoE secretion.
Molecular Cloning and Characterization of MbMYB108, a Malus baccata MYB Transcription Factor Gene, with Functions in Tolerance to Cold and Drought Stress in Transgenic Arabidopsis thaliana
Authors: Yao Chunya , Li Wenhui , Liang Xiaoqi , Ren Chuankun , Liu Wanda , Yang Guohui , Zhao Mengfei , Yang Tianyu , Li Xingguo , Han Deguo
Published: April 2022

The MYB transcription factor (TF) family is one of the largest transcription families in plants, which is widely involved in the responses of plants to biotic and abiotic stresses, as well as plant growth, development, and metabolic regulation. In the present study, a new MYB TF gene, MbMYB108, from Malus baccata (L.) Borkh, was identified and characterized. The open reading frame (ORF) of MbMYB108 was found to be 903 bp, encoding 300 amino acids. Sequence alignment results and predictions of the protein structure indicated that the MbMYB108 protein contained the conserved MYB domain. Subcellular localization showed that MbMYB108 was localized to the nucleus. The expression of MbMYB108 was enriched in young and mature leaves, and was highly affected by cold and drought treatments in M. baccata seedlings. When MbMYB108 was introduced into Arabidopsis thaliana, it greatly increased the cold and drought tolerances in the transgenic plant. Increased expression of MbMYB108 in transgenic A. thaliana also resulted in higher activities of peroxidase (POD) and catalase (CAT), higher contents of proline and chlorophyll, while malondialdehyde (MDA) content and relative conductivity were lower, especially in response to cold and drought stresses. Therefore, these results suggest that MbMYB108 probably plays an important role in the response to cold and drought stresses in A. thaliana by enhancing the scavenging capability for reactive oxygen species (ROS).
Emerging Function of Ecotype-Specific Splicing in the Recruitment of Commensal Microbiome
Authors: Li Yue-Han , Yang Yuan-You , Wang Zhi-Gang , Chen Zhuo
Published: April 2022

In recent years, host–microbiome interactions in both animals and plants has emerged as a novel research area for studying the relationship between host organisms and their commensal microbial communities. The fitness advantages of this mutualistic interaction can be found in both plant hosts and their associated microbiome, however, the driving forces mediating this beneficial interaction are poorly understood. Alternative splicing (AS), a pivotal post-transcriptional mechanism, has been demonstrated to play a crucial role in plant development and stress responses among diverse plant ecotypes. This natural variation of plants also has an impact on their commensal microbiome. In this article, we review the current progress of plant natural variation on their microbiome community, and discuss knowledge gaps between AS regulation of plants in response to their intimately related microbiota. Through the impact of this article, an avenue could be established to study the biological mechanism of naturally varied splicing isoforms on plant-associated microbiome assembly.
Mesenchymal Stem Cells and Extracellular Vesicles Derived from Canine Adipose Tissue Ameliorates Inflammation, Skin Barrier Function and Pruritus by Reducing JAK/STAT Signaling in Atopic Dermatitis
Authors: Kim Sung Youl , Yoon Tae Hong , Na Jungtae , Yi Seong Joon , Jin Yunseok , Kim Minji , Oh Tae-Ho , Chung Tae-Wook
Published: April 2022

Canine atopic dermatitis (AD) is a common chronic inflammatory skin disorder resulting from imbalance between T lymphocytes. Current canine AD treatments use immunomodulatory drugs, but some of the dogs have limitations that do not respond to standard treatment, or relapse after a period of time. Thus, the purpose of this study was to evaluate the immunomodulatory effect of mesenchymal stem cells derived from canine adipose tissue (cASCs) and cASCs-derived extracellular vesicles (cASC-EVs) on AD. First, we isolated and characterized cASCs and cASCs-EVs to use for the improvement of canine atopic dermatitis. Here, we investigated the effect of cASCs or cASC-EVs on DNCB-induced AD in mice, before using for canine AD. Interestingly, we found that cASCs and cASC-EVs improved AD-like dermatitis, and markedly decreased levels of serum IgE, (49.6%, p = 0.002 and 32.1%, p = 0.016 respectively) epidermal inflammatory cytokines and chemokines, such as IL-4 (32%, p = 0.197 and 44%, p = 0.094 respectively), IL-13 (47.4%, p = 0.163, and 50.0%, p = 0.039 respectively), IL-31 (64.3%, p = 0.030 and 76.2%, p = 0.016 respectively), RANTES (66.7%, p = 0.002 and 55.6%, p = 0.007) and TARC (64%, p = 0.016 and 86%, p = 0.010 respectively). In addition, cASCs or cASC-EVs promoted skin barrier repair by restoring transepidermal water loss, enhancing stratum corneum hydration and upregulating the expression levels of epidermal differentiation proteins. Moreover, cASCs or cASC-EVs reduced IL-31/TRPA1-mediated pruritus and activation of JAK/STAT signaling pathway. Taken together, these results suggest the potential of cASCs or cASC-EVs for the treatment of chronic inflammation and damaged skin barrier in AD or canine AD.
The Role of Hydrolases in Biology and Xenobiotics Metabolism
Authors: Morisseau Christophe
Published: April 2022

Chemical exposure can profoundly affect our health, some being voluntary (food and drugs) and some involuntary (environmental contaminants) [...]
CfDNA Measurement as a Diagnostic Tool for the Detection of Brain Somatic Mutations in Refractory Epilepsy
Authors: Mayo Sonia , Gómez-Manjón Irene , Fernández-Martínez Francisco Javier , Camacho Ana , Martínez Francisco , Benito-León Julián
Published: April 2022

Epilepsy is a neurological disorder that affects more than 50 million people. Its etiology is unknown in approximately 60% of cases, although the existence of a genetic factor is estimated in about 75% of these individuals. Hundreds of genes involved in epilepsy are known, and their number is increasing progressively, especially with next-generation sequencing techniques. However, there are still many cases in which the results of these molecular studies do not fully explain the phenotype of the patients. Somatic mutations specific to brain tissue could contribute to the phenotypic spectrum of epilepsy. Undetectable in the genomic DNA of blood cells, these alterations can be identified in cell-free DNA (cfDNA). We aim to review the current literature regarding the detection of somatic variants in cfDNA to diagnose refractory epilepsy, highlighting novel research directions and suggesting further studies.
Mutant lpa1 Analysis of ZmLPA1 Gene Regulates Maize Leaf-Angle Development through the Auxin Pathway
Authors: Ji Xiangzhuo , Gao Qiaohong , Chen Fenqi , Bai Mingxing , Zhuang Zelong , Peng Yunling
Published: April 2022

Maize plant type is one of the main factors determining maize yield, and leaf angle is an important aspect of plant type. The rice Loose Plant Architecture1 (LPA1) gene and Arabidopsis AtIDD15/SHOOT GRAVITROPISM5 (SGR5) gene are related to their leaf angle. However, the homologous ZmLPA1 in maize has not been studied. In this study, the changing of leaf angle, as well as gene expression in leaves in maize mutant lpa1 and wild-type ‘B73’ under different IAA concentrations were investigated. The regulation effect of IAA on the leaf angle of lpa1 was significantly stronger than that of the wild type. Transcriptome analysis showed that different exogenous IAA treatments had a common enrichment pathway—the indole alkaloid biosynthesis pathway—and among the differentially expressed genes, four genes—AUX1, AUX/IAA, ARF and SAUR—were significantly upregulated. This study revealed the regulation mechanism of ZmLPA1 gene on maize leaf angle and provided a promising gene resource for maize breeding.
Lung Extracellular Matrix Hydrogels Enhance Preservation of Type II Phenotype in Primary Alveolar Epithelial Cells
Authors: Marhuenda Esther , Villarino Alvaro , Narciso Maria Leonor , Camprubí-Rimblas Marta , Farré Ramon , Gavara Núria , Artigas Antonio , Almendros Isaac , Otero Jorge
Published: April 2022

One of the main limitations of in vitro studies on lung diseases is the difficulty of maintaining the type II phenotype of alveolar epithelial cells in culture. This fact has previously been related to the translocation of the mechanosensing Yes-associated protein (YAP) to the nuclei and Rho signaling pathway. In this work, we aimed to culture and subculture primary alveolar type II cells on extracellular matrix lung-derived hydrogels to assess their suitability for phenotype maintenance. Cells cultured on lung hydrogels formed monolayers and maintained type II phenotype for a longer time as compared with those conventionally cultured. Interestingly, cells successfully grew when they were subsequently cultured on a dish. Moreover, cells cultured on a plate showed the active form of the YAP protein and the formation of stress fibers and focal adhesions. The results of chemically inhibiting the Rho pathway strongly suggest that this is one of the mechanisms by which the hydrogel promotes type II phenotype maintenance. These results regarding protein expression strongly suggest that the chemical and biophysical properties of the hydrogel have a considerable impact on the transition from ATII to ATI phenotypes. In conclusion, culturing primary alveolar epithelial cells on lung ECM-derived hydrogels may facilitate the prolonged culturing of these cells, and thus help in the research on lung diseases.
A Novel High Content Angiogenesis Assay Reveals That Lacidipine, L-Type Calcium Channel Blocker, Induces In Vitro Vascular Lumen Expansion
Authors: Nawrot Dorota A. , Ozer Lutfiye Yildiz , Al Haj Zen Ayman
Published: April 2022

Angiogenesis is a critical cellular process toward establishing a functional circulatory system capable of delivering oxygen and nutrients to the tissue in demand. In vitro angiogenesis assays represent an important tool for elucidating the biology of blood vessel formation and for drug discovery applications. Herein, we developed a novel, high content 2D angiogenesis assay that captures endothelial morphogenesis’s cellular processes, including lumen formation. In this assay, endothelial cells form luminized vascular-like structures in 48 h. The assay was validated for its specificity and performance. Using the optimized assay, we conducted a phenotypic screen of a library containing 150 FDA-approved cardiovascular drugs to identify modulators of lumen formation. The screening resulted in several L-type calcium channel blockers being able to expand the lumen space compared to controls. Among these blockers, Lacidipine was selected for follow-up studies. We found that the endothelial cells treated with Lacidipine showed enhanced activity of caspase-3 in the luminal space. Pharmacological inhibition of caspase activity abolished the Lacidipine-enhancing effect on lumen formation, suggesting the involvement of apoptosis. Using a Ca2+ biosensor, we found that Lacipidine reduces the intracellular Ca2+ oscillations amplitude in the endothelial cells at the early stage, whereas Lacidipine blocks these Ca2+ oscillations completely at the late stage. The inhibition of MLCK exhibits a phenotype of lumen expansion similar to that of Lacidipine. In conclusion, this study describes a novel high-throughput phenotypic assay to study angiogenesis. Our findings suggest that calcium signalling plays an essential role during lumen morphogenesis. L-type Ca2+ channel blockers could be used for more efficient angiogenesis-mediated therapies.
Low-Dose SARS-CoV-2 S-Trimer with an Emulsion Adjuvant Induced Th1-Biased Protective Immunity
Authors: Liao Hung-Chun , Wu Wan-Ling , Chiang Chen-Yi , Huang Min-Syuan , Shen Kuan-Yin , Huang Yu-Ling , Wu Suh-Chin , Liao Ching-Len , Chen Hsin-Wei , Liu Shih-Jen
Published: April 2022

During the sustained COVID-19 pandemic, global mass vaccination to achieve herd immunity can prevent further viral spread and mutation. A protein subunit vaccine that is safe, effective, stable, has few storage restrictions, and involves a liable manufacturing process would be advantageous to distribute around the world. Here, we designed and produced a recombinant spike (S)-Trimer that is maintained in a prefusion state and exhibits a high ACE2 binding affinity. Rodents received different doses of S-Trimer (0.5, 5, or 20 μg) antigen formulated with aluminum hydroxide (Alum) or an emulsion-type adjuvant (SWE), or no adjuvant. After two vaccinations, the antibody response, T-cell responses, and number of follicular helper T-cells (Tfh) or germinal center (GC) B cells were assessed in mice; the protective efficacy was evaluated on a Syrian hamster infection model. The mouse studies demonstrated that adjuvating the S-Trimer with SWE induced a potent humoral immune response and Th1-biased cellular immune responses (in low dose) that were superior to those induced by Alum. In the Syrian hamster studies, when S-Trimer was adjuvanted with SWE, higher levels of neutralizing antibodies were induced against live SARS-CoV-2 from the original lineage and against the emergence of variants (Beta or Delta) with a slightly decreased potency. In addition, the SWE adjuvant demonstrated a dose-sparing effect; thus, a lower dose of S-Trimer as an antigen (0.5 μg) can induce comparable antisera and provide complete protection from viral infection. These data support the utility of SWE as an adjuvant to enhance the immunogenicity of the S-Trimer vaccine, which is feasible for further clinical testing.
Hepatoprotective Effects of Albumin-Encapsulated Nanoparticles of a Curcumin Derivative COP-22 against Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Injury in Mice
Authors: Mu Wenwen , Wang Qi , Jia Mingxia , Dong Sijia , Li Sijie , Yang Jie , Liu Guoyun
Published: April 2022

Acute liver injury (ALI) is a severe syndrome and can further develop into acute liver failure (ALF) which can lead to high mortality and cause irreversible liver injuries in the clinic. Liver transplantation is the most common treatment; however, liver donors are lacking, and the progression of ALF is rapid. Nanoparticles can increase the bioavailability and the targeted accumulation of drugs in the liver, so as to significantly improve the therapeutic effect of ALI. Curcumin derivative COP-22 exhibits low cytotoxicity and effective anti-inflammatory activity; however, it has poor water solubility. In this study, COP-22-loaded bovine serum albumin (BSA) nanoparticles (22 NPs) were prepared and characterized. They exhibit effective hepatoprotective effects by inhibiting inflammation, oxidative stress, and apoptosis on Lipopolysaccharide/D-Galactosamine-induced acute liver injury of mice. The anti-inflammatory activity of 22 NPs is related to the regulation of the NF-κB signaling pathways; the antioxidant activity is related to the regulation of the Nrf2 signaling pathways; and the apoptosis activity is related to mitochondrial pathways, involving Bcl-2 family and Caspase-3 protein. These three cellular pathways are interrelated and affected each other. Moreover, 22 NPs could be passively targeted to accumulate in the liver through the retention effect and are more easily absorbed than 22.HCl salt in the liver.
Modulation of Mismatch Repair and the SOCS1/p53 Axis by microRNA-155 in the Colon of Patients with Primary Sclerosing Cholangitis
Authors: Adamowicz Monika , Stukan Iga , Milkiewicz Piotr , Bialek Andrzej , Milkiewicz Malgorzata , Kempinska-Podhorodecka Agnieszka
Published: April 2022

Deficient mismatch repair (MMR) proteins may lead to DNA damage and microsatellite instability. Primary sclerosing cholangitis (PSC) is a risk factor for colitis-associated colon cancer. MiR-155 is suggested to act as a key regulating node, linking inflammation and tumorigenesis. However, its involvement in the chronic colitis of PSC-UC patients has not been examined. We investigated the involvement of miR-155 in the dysregulation of MMR genes and colitis in PSC patients. Colon tissue biopsies were obtained from patients with PSC, PSC with concomitant ulcerative colitis (PSC-UC), uncomplicated UC, and healthy controls (n = 10 per group). In the ascending colon of PSC and PSC-UC patients, upregulated miR-155 promoted high microsatellite instability and induced signal transducer and activator of transcription 3 (STAT-3) expression via the inhibition of suppressors of cytokine signalling 1 (SOCS1). In contrast, the absence of miR-155 overexpression in the sigmoid colon of PSC-UC patients activated the Il-6/S1PR1 signalling pathway and imbalanced the IL17/FOXP3 ratio, which reinforces chronic colitis. Functional studies on human intestinal epithelial cells (HT-29 and NCM460D) confirmed the role of miR-155 over-expression in the inhibition of MMR genes and the modulation of p53. Moreover, those cells produced more TNFα upon a lipopolysaccharide challenge, which led to the suppression of miR-155. Additionally, exposure to bile acids induced upregulation of miR-155 in Caco-2 cell lines. Thus, under different conditions, miR-155 is involved in either neoplastic transformation in the ascending colon or chronic colitis in the sigmoid colon of patients with PSC. New insight into local modulation of microRNAs, that may alter the course of the disease, could be used for further research on potential therapeutic applications.