bluuebunny/crossref_metadata_2025 huggingface dataset:
Technical–Economic Analysis of Renewable Hydrogen Production from Solar Photovoltaic and Hydro Synergy in a Pilot Plant in Brazil
Authors: Barros Souza Riedel Ana Beatriz , Feitosa Riedel Vitor , Souza Filho Hélio Nunes de , da Silva Ennio Peres , Marques Cabral Renato , de Brito Silva Leandro , de Castro Pereira Alexandre
Published: September 2024

Renewable hydrogen obtained from renewable energy sources, especially when produced through water electrolysis, is gaining attention as a promising energy vector to deal with the challenges of climate change and the intermittent nature of renewable energy sources. In this context, this work analyzes a pilot plant that uses this technology, installed in the Itumbiara Hydropower Plant located between the states of Goiás and Minas Gerais, Brazil, from technical and economic perspectives. The plant utilizes an alkaline electrolyzer synergistically powered by solar photovoltaic and hydro sources. Cost data for 2019, when the equipment was purchased, and 2020–2023, when the plant began continuous operation, are considered. The economic analysis includes annualized capital, maintenance, and variable costs, which determines the levelized cost of hydrogen (LCOH). The results obtained for the pilot plant’s LCOH were USD 13.00 per kilogram of H2, with an efficiency loss of 2.65% for the two-year period. Sensitivity analysis identified the capacity factor (CF) as the main determinant of the LCOH. Even though the analysis specifically applies to the Itumbiara Hydropower Plant, the CF can be extrapolated to larger plants as it directly influences hydrogen production regardless of plant size or capacity.
A Comparative Study of the Hydrogen Auto-Ignition Process in Oxygen–Nitrogen and Oxygen–Water Vapor Oxidizer: Numerical Investigations in Mixture Fraction Space and 3D Forced Homogeneous Isotropic Turbulent Flow Field
Authors: Caban Lena , Tyliszczak Artur
Published: September 2024

In this paper, we analyze the auto-ignition process of hydrogen in a hot oxidizer stream composed of oxygen–nitrogen and oxygen–water vapor with nitrogen/water vapor mass fractions in a range of 0.1–0.9. The temperature of the oxidizer varies from 1100 K to 1500 K and the temperature of hydrogen is assumed to be 300 K. The research is performed in 1D mixture fraction space and in a forced homogeneous isotropic turbulent (HIT) flow field. In the latter case, the Large Eddy Simulation (LES) method combined with the Eulerian Stochastic Field (ESF) combustion model is applied. The results obtained in mixture fraction space aim to determine the most reactive mixture fraction, maximum flame temperature, and dependence on the scalar dissipation rate. Among others, we found that the ignition in H2-O2-H2O mixtures occurs later than in H2-O2-N2 mixtures, especially at low oxidizer temperatures. On the other hand, for a high oxidizer temperature, the ignitability of H2-O2-H2O mixtures is extended, i.e., the ignition occurs for a larger content of H2O and takes place faster. The 3D LES-ESF results show that the ignition time is virtually independent of initial conditions, e.g., randomness of an initial flow field and turbulence intensity. The latter parameter, however, strongly affects the flame evolution. It is shown that the presence of water vapor decreases ignitability and makes flames more prone to extinction.
Potential of Wake Scaling Techniques for Vertical-Axis Wind Turbine Wake Prediction
Authors: Vahidi Dara , Porté-Agel Fernando
Published: September 2024

Analytical wake models are widely used to predict wind turbine wakes. While these models are well-established for horizontal-axis wind turbines (HAWTs), the analytical wake models for vertical-axis wind turbines (VAWTs) remain under-explored in the wind energy community. In this study, the accuracy of two wake scaling techniques is evaluated to predict the change in the normalized maximum wake velocity deficit behind VAWTs by re-scaling the maximum wake velocity deficit behind an actuator disk with the same thrust coefficient. The wake scaling is defined in terms of equivalent diameter, considering the geometrical properties of the wake-generating object. Two different equivalent diameters are compared, namely the momentum diameter and hydraulic diameter. Different approaches are used to calculate the change in the normalized wake velocity deficit behind a disk with the same thrust coefficient as the VAWT. The streamwise distance is scaled with the equivalent diameter to predict the normalized maximum wake velocity deficit behind the desired VAWT. The performance of the proposed framework is assessed using large-eddy simulation data of VAWTs operating in a turbulent boundary layer with varying operating conditions and aspect ratios. For all of the cases, the momentum diameter scaling provides reasonable predictions of the VAWT normalized maximum wake velocity deficit.
Numerical Investigation of Wake Characteristics for Scaled 20 kW Wind Turbine Models with Various Size Factors
Authors: Bazher Salim Abdullah , Park Juyeol , Oh Jungkeun , Seo Daewon
Published: September 2024

Wind energy is essential for sustainable energy development, providing a clean and reliable energy source through the wind turbine. However, the vortices and turbulence generated as wind passes through turbines reduce wind speed and increase turbulence, leading to significant power losses for downstream turbines in wind farms. This study investigates wake characteristics in wind turbines by examining the effects of different scale ratios on wake dynamics, using both experimental and numerical approaches, utilizing scaled-down models of the Aeolos H-20 kW turbine at scales of 1:33, 1:50, and 1:67. The experimental component involved wind tunnel tests in an open-circuit tunnel with adjustable wind speeds and controlled turbulence intensity. Additionally, Computational Fluid Dynamics (CFD) simulations were conducted using STAR-CCM+ (Version 15.06.02) to numerically analyze the wake characteristics. Prior to the simulation, a convergence test was performed by varying grid density and y+ values to establish optimized simulation settings essential for accurately capturing wake dynamics. The results were validated against experimental data, reinforcing the reliability of the simulations. Despite minor inconsistencies in areas affected by tower and nacelle interference, the overall results strongly support the methodology’s effectiveness. The discrepancies between the experimental results and CFD simulations underscore the limitations of the rigid body assumption, which does not fully account for the deformation observed in the experiment.
Chemical Ecology in Forests
Authors: Zhao Chunjian , Xia Zhi-Chao , Li Chunying , Zhu Jingle
Published: September 2024

There is a competitive and coordinated relationship among organisms which depends on their chemical connections [...]
Heat and Drought Have Exacerbated the Midday Depression Observed in a Subtropical Fir Forest by a Geostationary Satellite
Authors: Xie Qianqian , Chen Kexin , Li Tong , Liu Jia , Wang Yuqiu , Zhou Xiaolu
Published: September 2024

Recently, increasing heat and drought events have threatened the resilience of Chinese fir forests. Trees primarily respond to these threats by downregulating photosynthesis including through stomatal limitation that causes a drop in productivity at noon (known as the midday depression). However, the effects of these events on midday and afternoon GPP inhibition are rarely analyzed on a fine timescale. This may result in negligence of critical responses. Here, we investigated the impact of climatic events on the midday depression of photosynthesis at a subtropical fir forest in Huitong from 2016 to 2022 using data from the Himawari 8 meteorological satellite and flux tower. Our results indicated that the highest number of midday depression occurred in 2022 (126 times) with the highest average temperature (29.1 °C). A higher incidence of midday depression occurred in summer and autumn, with 48 and 34 occurrences, respectively. Compound drought, heat, and drought events induced increases in midday depression at 74.3%, 66.0%, and 47.5%. Thus, trees are more likely to adopt midday depression as an adaptive strategy during compound drought and heat events. This study can inform forest management and lead to improvements in Earth system models.
On Canopy Rainfall Interception Modeling in a Eucalyptus Plantation
Authors: Melo Neto José O. , Rodrigues André F. , Mello Carlos R.
Published: September 2024

The interaction between the forest canopy and precipitation is a fundamental process for understanding the hydrological cycle in forests. Physical models have been applied to estimate canopy water interception, and their efficiency has been tested based on metrics used to assess hydrological models. For eucalyptus plantations in Brazil, more studies are needed on the canopy rainfall interception model. Thus, we calibrated the Gash model using two complete hydrological years of observation in a eucalyptus plantation in southeastern Brazil. The model’s parametrization was conducted using 17 trees individually in different planting spacings (3 m × 2 m, 3 m × 3 m, and 3 m × 5 m). The average values of the model’s parameters were taken to represent the forest, and the average parameters for each planting spacing were used to assess the model’s performance according to the planting spacings. We used NSE, KGE, and Pbias statistical metrics to assess the model’s performance. For individual trees and rainfall events, the model showed an average NSE and Pbias of 0.59 and 18.2%, respectively, meaning a “satisfactory” performance for eight trees and “poor” performance for nine trees. When the model was averaged for the entire forest and individual rainfall events were considered, the metrics were improved, being 0.643 for NSE and 8.2% for Pbias, indicating a “good” model performance, which was strengthened by an average KGE of 0.746. Regarding the model for the planting spacings, the best results were found for the 3.0 m × 2.0 m spacing (“a good performance”). For the other spacings, Pbias was higher than 15%, leading to inferior performance, but with the NSE and KGE compatible with “good” performance. The practical implications of our findings are significant, as they can be used to enhance the accuracy of models for a better understanding of the hydrological cycle in eucalyptus forests in Brazil, thereby contributing to more effective forest management and conservation.
Variations in the Sensory Attributes of Infant Formula among Batches and Their Impact on Maternal Consumer Preferences: A Study Combining Consumer Preferences, Pivot Profile, and Quantitative Descriptive Analysis
Authors: Li Yilin , Hu Xinyu , Li Ruotong , Wang Chunguang , Wang Houyin , Liu Guirong , Gao Lipeng , Jin Anwen , Zhu Baoqing
Published: September 2024

The sensory quality of infant formula (IF) has a significant impact on the preferences and purchasing behavior of maternal consumers. Consumer-based rapid descriptive methods have become popular and are widely preferred over classical methods, but the application of Pivot Profile (PP) in IF is still little explored. In this study, both Pivot Profile (PP) and Quantitative Descriptive Analysis (QDA) were applied to characterize the sensory profile of 12 batches of one-stage and three-stage IF with different storage periods, respectively, along with consumer preference data to determine the flavors contributing to liking. The results of PP and QDA aligned moderately well, with the most perceptible differences identified as “fishy”, “milky”, and “T-sweet” attributes. IFs with shorter storage times were highly associated with “milky” aromas and “T-sweet” tastes, whereas IFs with longer storage times exhibited a strong correlation with “fishy” and “oxidation” aromas. External preference analysis highlighted that the occurrence of “fishy” and “oxidation” aromas during prolonged storage periods significantly reduced the consumer preference for IFs. Conversely, the perception of “milky” and “creamy” aromas and “T-sweet” tastes may be critical positive factors influencing consumer preference. This study provided valuable insights and guidance for enhancing the sensory quality and consumer preference of IF.
Vacuum Packaging Can Protect Ground Beef Color and Oxidation during Cold Storage
Authors: Bernardez-Morales Gabriela M. , Douglas Savannah L. , Nichols Brooks W. , Barrazueta-Cordero Ricardo J. , Belk Aeriel D. , Brandebourg Terry D. , Reyes Tristan M. , Sawyer Jason T.
Published: September 2024

Storing ground beef at frozen temperatures prior to refrigerated display when using thermoforming vacuum packaging is not a common manufacturing practice. However, limited data on thermoforming packaging film and its interaction with meat quality suggests that more information is needed. The current study aimed to identify the influences of thermoforming packaging on the surface color and lipid oxidation of ground beef. Ground beef was portioned into 454 g bricks and packaged into one of three thermoforming films: T1 (150 µ polyethylene/EVOH/polyethylene coextrusion), T2 (175 µ polyethylene /EVOH/polyethylene coextrusion), and T3 (200 µ polyethylene/EVOH/polyethylene coextrusion), stored for 21 days at −20.83 °C (±1.50 °C), and displayed for 42 days at 3.0 °C ± 1.5 °C. There were no statistical differences for the packaging treatment of lipid oxidation (p = 0.0744), but oxidation increased throughout storage day (p < 0.0001). The main effects of treatment and day resulted in altered (p < 0.05) surface lightness (L*), redness (a*), yellowness, hue angle (°), red-to-brown (RTB), and relative myoglobin for met-myoglobin (MET), deoxymyoglobin (DMB), and oxymyoglobin (OMB). Surprisingly, there was an interaction between treatment and day for the calculated relative values of chroma (p = 0.0321), Delta E (p = 0.0155), and the ratio of a*:b* (p < 0.0001). These results indicate that thermoforming vacuum packaging can reduce the rate of deterioration that occurs to ground beef color and the rate of oxidation.
Yellow Teas Protect against DSS-Induced Ulcerative Colitis by Inhibiting TLR4/NF-κB/NLRP3 Inflammasome in Mice
Authors: Xing Dawei , Zheng Tao , Chen Xiaoju , Xie Zhongwen
Published: September 2024

Yellow tea (YT), a slightly fermented tea with a unique yellowing process and mellow taste, is becoming widely popular. Currently, the YT includes bud yellow tea (BYT), small-leaf yellow tea (SYT), and large-leaf yellow tea (LYT) based on maturity of raw materials. Previous studies have shown that YT has outstanding potential in preventing metabolic syndrome. However, the distinct effects and mechanisms of different types of YT on ulcerative colitis (UC) are still unclear. This study investigated the effects and mechanisms of continuous or intermittent intervention of three yellow tea water extracts (YTEs) on dextran sulfate sodium (DSS)-induced ulcerative colitis in CD-1 mice. The results showed that YTE intervention significantly improves the syndrome of DSS-induced UC in mice. Mechanistic studies reveal that YTEs increase the expression levels of tight junction (TJ) proteins and reduce the levels of pro-inflammatory cytokines in the colon by inactivating TLR4/NF-κB/NLRP3. YTE treatment protected intestinal barrier integrity and reduced serum lipopolysaccharide (LPS) levels. Interestingly, our results indicate that large-leaf yellow tea (LYT) has a better alleviating effect than BYT and SYT. YTE intervention before DSS administration has a certain degree of preventive effect on ulcerative colitis, while continuous YTE intervention after DSS induction has a significant reversing effect on the damage caused by DSS. Our results indicated that drinking YT may have preventive and therapeutic effect on UC, especially drinking LYT.
Sustainable Jam with Apple Pomace: Gelling, Rheology, and Composition Analysis
Authors: Viegas Ândria , Alegria Maria João , Raymundo Anabela
Published: September 2024

Fruit juice processing can generate significant waste, but efficiently repurposing some of its byproducts not only reduces environmental impact but also adds value, thereby enhancing sustainability in the food industry. This work assesses the use of hydrocolloids in jam preparation and the influence of time and temperature on gelation in the presence of apple pomace. The effects of different processing conditions were analyzed using response surface methodology. Viscosity, elastic modulus (G′), viscous modulus (G″), and firmness were measured. Results indicated that both time and temperature significantly improved rheological and textural properties. The optimal conditions (35.6 min and 84.2 °C) yielded a viscosity of 3.66 × 10⁴ ± 4.49 × 102 Pa·s and a G′ at 1 Hz of 2596 ± 128 Pa. The final product exhibited the desirable texture, was free of added sugars, had low lipid content, and retained its bioactive compounds. Applying apple pomace in the formulation allows a more efficient hydrocolloid system, promotes a circular economy, and combats food waste.
Development and Investigation of a Nanoemulgel Formulated from Tunisian Opuntia ficus-indica L. Seed Oil for Enhanced Wound Healing Activity
Authors: Bahloul Badr , Ben Bnina Enis , Dridi Dorra , Bouhamed Aya , Castillo Henríquez Luis , Flamini Guido , Vega-Baudrit José Roberto
Published: September 2024

The aim of this study is to develop a nanoemulgel encapsulating a Tunisian Prickly Pear (Opuntia ficus-indica L.) seed oil (PPSO) to assess, for the first time, the in vivo efficacy of this nanoformulation on wound healing. Phytocompounds of this oil have been reported in the literature as having powerful pharmacological activities. However, it remains poorly exploited due to low bioavailability. A nanoemulsion (NE) was designed by determining the required hydrophilic–lipophilic balance (HLB) and subsequently characterized. The mean droplet size was measured at 56.46 ± 1.12 nm, with a polydispersity index (PDI) of 0.23 ± 0.01 using dynamic light scattering. The zeta potential was −31.4 ± 1.4 mV, and the morphology was confirmed and assessed using transmission electron microscopy (TEM). These characteristics align with the typical properties of nanoemulsions. The gelification process resulted in the formation of a nanoemulgel from the optimum nanoemulsion. The high wound healing efficiency of the nanoemulgel was confirmed compared to that of a medicinally marketed cream. The outcomes of this research contribute valuable insights, for the first time, into the potential therapeutic applications of PPSO and its innovative pharmaceutical formulation for wound healing.
Factors Associated with Increased Burden of Caregivers of People with Dementia with Lewy Bodies
Authors: Toya Shunji , Hashimoto Mamoru , Manabe Yuta , Yamakage Hajime , Ikeda Manabu
Published: September 2024

The burden of caregivers of people with dementia with Lewy bodies (DLB) is high; however, factors related to their caregiving burden are not fully clarified. We herein investigated factors associated with increasing caregiver burden for caregivers of people with DLB. To explore factors associated with caregiver burden, a linear regression analysis was conducted using the J-ZBI_8 total score as the dependent variable and a total of 36 factors as independent variables. This analysis included 252 pairs of people with DLB and their caregivers. Caregivers’ mean J-ZBI_8 was 8.4, indicating that caregiver burden was generally high. First, we identified 20 factors associated with caregiver burden in univariable analysis. Finally, multivariable analysis found three significant factors: irritability (β = 0.208, p < 0.001), use of “short stay” or “small-scale, multifunctional home care” (β = 0.208, p < 0.001), and nighttime behavior (β = 0.138, p = 0.020) were significantly associated with J-ZBI_8 total scores. Irritability and nighttime behavior were found to be contributing factors to caregiver burden. High caregiver burden among caregivers of people with DLB may result in the use of social services providing overnight stays, but to what extent such services reduce caregiver burden is unknown.
Psychometric Analysis of the Hip Disability and Osteoarthritis Outcome Score (HOOS)
Authors: Miley Emilie N. , Casanova Madeline P. , Pickering Michael A. , Cheatham Scott W. , Larkins Lindsay W. , Cady Adam C. , Baker Russell T.
Published: September 2024

Hip Disability and Osteoarthritis Outcome Survey (HOOS) was developed as a region- and disease-specific outcome to assess hip disability. Despite the use of the HOOS in clinical practice and research, psychometric analyses of the scale in a large dataset of patients have not been performed. As such, the purposes of this study were to assess the structural validity of the HOOS in patients who underwent a total hip arthroplasty. Data were obtained from the Surgical Outcome System (SOS) global registry. Confirmatory factor analysis (CFA) was conducted to assess the scale structure of the 40-item HOOS and exploratory factor analysis (EFA) was conducted to identify a parsimonious scale structure. The parsimonious model identified was subjected to multi-group and longitudinal invariance testing and LGC modeling. The original five-factor, 40-item HOOS did not meet recommended model fit indices values (CFI = 0.822, TLI = 0.809, IFI = 0.822, RMSEA = 0.085). Alternate model generation identified an alternative model (i.e., HOOS-9). Sound model fit was identified for the HOOS-9 (CFI = 0.974, TLI = 0.961, RMSEA = 0.046). Invariance testing criteria were also met between groups (i.e., age and sex) and across time. Lastly, a nonlinear growth trajectory was identified in responses pertaining to hip disability. The original scale structure of the 40-item HOOS was not supported. The HOOS-9 met contemporary model fit recommendations, along with multi-group and longitudinal invariance testing. Our findings support the preliminary use of the HOOS-9 to assess hip function and disability in research and clinical practice.
Perceived Pressures and Mental Health of Breastfeeding Mothers: A Qualitative Descriptive Study
Authors: Wheeler Abigail , Farrington Shanti , Sweeting Fay , Brown Amy , Mayers Andrew
Published: September 2024

When a mother is supported to breastfeed, the benefits for her mental health are significant. However, if pressured or unsupported, the opposite is true. This research examines mothers’ breastfeeding experiences, exploring how perceived pressure can impact perinatal mental health. A sample of 501 respondents to a research questionnaire was explored using Reflexive Thematic Analysis. Three main themes identified were perceived pressure to breastfeed, perceived pressure not to breastfeed and mental health impact. The main findings were that mothers received conflicting advice from healthcare professionals, and pressures to feed in a certain way came from their support networks, as well as from their internal beliefs. Perceived pressures negatively impacted maternal mental health, while positive breastfeeding experiences benefitted mental health outcomes.
Climate Change Challenge Faced by Italian Children: A Nationwide Study
Authors: Provenzi Livio , Ovalles Gomez Michelle A. , Frassone Simona , Pilo Cinzia , Angiolini Elisa , Barello Serena
Published: September 2024

Climate change threatens environmental stability and human health, with limited research on younger children’s perceptions. This study examines Italian primary school children’s views on climate change to guide educational and policy strategies. Surveying 973 children (5–11 years) from different regions, findings showed high awareness (93%) and concern (63%) about climate change. Regional differences indicated greater concern in the south. Gender disparities revealed females were more concerned and experienced more sleep difficulties. Younger children had stronger emotional responses, while older ones showed increased pro-environmental behaviors. Despite moderate self-confidence in effecting change, a strong sense of responsibility and trust in adults was prevalent. These results highlight the need for early, continuous climate education tailored to regional, age, and gender-specific needs. Addressing children’s views on climate change can help educators and policymakers foster resilience and proactive attitudes, supporting the development of informed and engaged future stewards of the planet.
Effects of Tree Leaves, Tannins, and Water Color on Chlorophyll Concentrations in Ponds
Authors: Oliva Anna , Doolittle Christina M. , Medlock Shelby A. , Aubert Joseph F. , Earl Julia E.
Published: September 2024

Leaf litter is an important input to freshwater systems. Leaves provide carbon, nutrients, and secondary compounds. We examined the effects of tree leaf species on chlorophyll a concentration—a proxy for phytoplankton biomass. We found that an input of Chinese tallow (Triadica sebiferum, invasive in the southeastern USA) and red maple (Acer rubrum) leaves resulted in lower chlorophyll concentrations than controls and other native species. These leaf species also leached tannins, resulting in a darker water color, and either may have caused the patterns observed. To separate these potential mechanisms (darker water leading to light limitation and tannin toxicity), we conducted a second experiment with a fully factorial design manipulating tannins and water color. We found that darker water resulted in the lowest chlorophyll concentration, suggesting light limitation. In the clear-water treatment, the addition of tannic acid lowered chlorophyll concentrations but also resulted in moderately darker water by the end of the experiment. The tannic acid may have been toxic to the algae, or there may have been some light limitation. Our results suggest that tannins that darken water color may substantially suppress phytoplankton and that tree species composition may influence both phytoplankton and the brownification of freshwater.
The Effect of Parents’ Nutrition Literacy on Children’s Oral-Health-Related Quality of Life
Authors: Aslan Gonul Busra , Cicek Betul
Published: September 2024

Parents can help children adopt good eating habits early in childhood by encouraging them to eat healthy. While parents’ levels of nutritional literacy is known to play a role in children’s healthy nutrition, healthy food is also essential in improving oral-health-related quality of life (OHRQoL). Since the effect of parents’ nutritional literacy on children’s OHRQoL is not yet known, this study aimed to examine the impact of parental nutritional literacy on children’s OHRQoL. This study was conducted with 459 parents of children aged 3–6 living in the Central Anatolia Region of Türkiye. Data collection tools included a sociodemographic form, the Early Childhood Oral Health Impact Scale (ECOHIS) and the Evaluation Instrument of Nutrition Literacy on Adults (EINLA). Data were analyzed in SPSS, using Student’s t-test, Chi-square test, and binary logistic regression models. Parents with higher nutritional literacy tend to have higher levels of education. An increase in total nutritional literacy score, especially in the general nutrition knowledge (GNK) and food label and numerical literacy (FLNL) subscales, was associated with higher child OHRQoL. Increasing parents’ nutritional literacy levels can contribute to OHRQoL by enabling them to be good role models for their children. Therefore, increasing parents’ nutritional literacy can improve OHRQoL by improving children’s nutrition and can be considered a protective factor for oral health.
Athlete Health Implications of Match Injuries in Portuguese Rugby Union
Authors: Lopes Nuno Pinto de Sousa , Cruz-Ferreira António Miguel , Lima Diana Torres , Silva Marta Amaral , Santiago Luiz Miguel
Published: September 2024

Objective: Our aim was to report time-loss match injuries in Portugal’s “Divisão de Honra” (Portuguese first tier) in the 2022/2023 season, focusing on injury incidence, burden, anatomical region, and type, to assess their impact on athletes’ health and availability to play. Methods: A prospective cohort study was conducted monitoring injuries via an online form filled in by each team’s medical department, categorizing player position, as well as injury type, location, and severity. Incidence and burden were calculated per 1000 player-match-hours. Results: The overall injury incidence was 54.4 injuries per 1000 player-match-hours (95% CI 30.3–96.2). Forwards had higher injury incidence than backs. Lower limbs were the most affected locations—29 injuries/1000 h of exposure (95% CI 13.7–52.0). Sprain/ligaments injuries were the most common type of injury (14.4 injuries/1000 h (95% CI 3.7–30.0), followed by muscle injuries (8.1 injuries/1000 h 95% CI 4.9–11.5). Discussion: Injury incidence was higher than what is reported in amateur competitions and lower than for professionals. Injury severity was similar to that in other studies. Our study suggests a lower injury burden than in other semi-professional and elite leagues. These findings highlight the need for targeted injury prevention strategies in the Portuguese Rugby Union to increase athletes’ availability by decreasing injury incidence and/or burden. Further research with broader participation and training injury data is needed.
A Comparison of an Australian First Nations Primary Healthcare Data Specification with Potentially Preventable Hospitalisations
Authors: Potts Boyd , Doran Christopher M. , Begg Stephen J.
Published: September 2024

Potentially Preventable Hospitalisations (PPH) is a widely used indicator of the effectiveness of non-hospital care. Specified using the International Classification of Diseases (ICD) coding, PPH comprises a suite of health conditions that could have potentially been prevented with appropriate care. The most recent edition of the National Guide to a Preventative Health Assessment for First Nations People documents the health conditions of interest to providers of primary care, many of which are not represented in PPH. Given the National Guide has been developed specifically with First Nations in mind, the aim of this research is twofold. The first aim is to formally posit the question of whether a summative measure of hospitalisations aligned diagnostically to the National Guide has value either as an alternative or complement to PPH in the context of First Nations primary health information. The second aim is to develop and present a prototype ICD-10 data specification for such a measure, referred to as the First Nations primary healthcare (FNPHC) data specification, and examine the age-standardised hospitalisation rates for FNPHC and PPH for correlations and/or differences. Age-standardised hospitalisation rates from 2016–17 to 2019–20 using both classifications were examined to assess the usefulness and relevance of summative measures of hospitalisations for informing primary care. Rates of FNPHC for principal diagnoses were between 1.5 and 2.5 times higher than those of PPH and approximately between 6 and 12 times higher for additional diagnoses. There was a strong correlation with PPH when rates were compared across all observations: jurisdictions with higher rates of PPH tended to have higher rates of hospitalisations according to the custom specification. Findings support its application as a summary measure for First Nations primary care providers. Given the policy landscape in Australia that aims to close the gap, it is imperative that measures of primary health take advantage of the concepts and application of First Nations data sovereignty and governance. The validity and cultural appropriateness of the First Nations primary health data specification needs to be further researched.
Alzheimer’s Disease Pathology and Assistive Nanotheranostic Approaches for Its Therapeutic Interventions
Authors: Dey Anuvab , Ghosh Subhrojyoti , Rajendran Ramya Lakshmi , Bhuniya Tiyasa , Das Purbasha , Bhattacharjee Bidyabati , Das Sagnik , Mahajan Atharva Anand , Samant Anushka , Krishnan Anand , Ahn Byeong-Cheol , Gangadaran Prakash
Published: September 2024

Alzheimer’s disease (AD) still prevails and continues to increase indiscriminately throughout the 21st century, and is thus responsible for the depreciating quality of health and associated sectors. AD is a progressive neurodegenerative disorder marked by a significant amassment of beta-amyloid plaques and neurofibrillary tangles near the hippocampus, leading to the consequent loss of cognitive abilities. Conventionally, amyloid and tau hypotheses have been established as the most prominent in providing detailed insight into the disease pathogenesis and revealing the associative biomarkers intricately involved in AD progression. Nanotheranostic deliberates rational thought toward designing efficacious nanosystems and strategic endeavors for AD diagnosis and therapeutic implications. The exceeding advancements in this field enable the scientific community to envisage and conceptualize pharmacokinetic monitoring of the drug, sustained and targeted drug delivery responses, fabrication of anti-amyloid therapeutics, and enhanced accumulation of the targeted drug across the blood–brain barrier (BBB), thus giving an optimistic approach towards personalized and precision medicine. Current methods idealized on the design and bioengineering of an array of nanoparticulate systems offer higher affinity towards neurocapillary endothelial cells and the BBB. They have recently attracted intriguing attention to the early diagnostic and therapeutic measures taken to manage the progression of the disease. In this article, we tend to furnish a comprehensive outlook, the detailed mechanism of conventional AD pathogenesis, and new findings. We also summarize the shortcomings in diagnostic, prognostic, and therapeutic approaches undertaken to alleviate AD, thus providing a unique window towards nanotheranostic advancements without disregarding potential drawbacks, side effects, and safety concerns.
Functional Analysis of RE1 Silencing Transcription Factor as a Putative Tumor Suppressor in Human Endometrial Cancer
Authors: Abedin Yasmin , Minchella Paige , Peterson Riley , Gonnella Francesca , Graham Amanda , Cook Ian , Javellana Melissa , Jewell Andrea , Spoozak Lori , Nothnick Warren B.
Published: September 2024

Uterine cancer is the most common gynecologic malignancy in the United States, with endometrioid endometrial adenocarcinoma (EC) being the most common histologic sub-type. Considering the molecular classifications of EC, efforts have been made to identify additional biomarkers that can assist in diagnosis, prognosis, and individualized therapy. We sought to explore the relationship of Repressor Element 1 (RE1) silencing transcription factor (REST), which downregulates neuronal genes in non-neuronal tissue, along with matrix metalloproteinase-24 (MMP24) and EC. We analyzed the expression of REST and MMP24 in 31 cases of endometrial cancer and 16 controls. We then explored the baseline expression of REST and MMP24 in two EC cell lines (Ishikawa and HEC-1-A) compared to a benign cell line (t-HESC) and subsequently evaluated proliferation, migration, and invasion in the setting of loss of REST gene expression. REST and MMP24 expression were significantly lower in human EC samples compared to control samples. REST was highly expressed in EC cell lines, but decreasing REST gene expression increased proliferation (FC: 1.13X, p < 0.0001), migration (1.72X, p < 0.0001), and invasion (FC: 7.77X, p < 0.05) in Ishikawa cells, which are hallmarks of cancer progression and metastasis. These findings elicit a potential role for REST as a putative tumor suppressor in EC.
New Advances in Metastatic Urothelial Cancer: A Narrative Review on Recent Developments and Future Perspectives
Authors: Tonni Elena , Oltrecolli Marco , Pirola Marta , Tchawa Cyrielle , Roccabruna Sara , D’Agostino Elisa , Matranga Rossana , Piombino Claudia , Pipitone Stefania , Baldessari Cinzia , Bacchelli Francesca , Dominici Massimo , Sabbatini Roberto , Vitale Maria Giuseppa
Published: September 2024

The standard of care for advanced or metastatic urothelial carcinoma (mUC) was historically identified with platinum-based chemotherapy. Thanks to the advances in biological and genetic knowledge and technologies, new therapeutic agents have emerged in this setting recently: the immune checkpoint inhibitors and the fibroblast growth factor receptor inhibitors as the target therapy for patients harboring alterations in the fibroblast growth factor receptor (FGFR) pathway. However, chasing a tumor’s tendency to recur and progress, a new class of agents has more recently entered the scene, with promising results. Antibody–drug conjugates (ADCs) are in fact the latest addition, with enfortumab vedotin being the first to receive accelerated approval by the U.S. Food and Drug Administration in December 2019, followed by sacituzumab govitecan. Many other ADCs are still under investigation. ADCs undoubtedly represent the new frontier, with the potential of transforming the management of mUC treatment in the future. Therefore, we reviewed the landscape of mUC treatment options, giving an insight into the molecular basis and mechanisms, and evaluating new therapeutic strategies in the perspective of more and more personalized treatments.
Comparison between USPIOs and SPIOs for Multimodal Imaging of Extracellular Vesicles Extracted from Adipose Tissue-Derived Adult Stem Cells
Authors: Capuzzo Arnaud M. , Piccolantonio Giusi , Negri Alessandro , Bontempi Pietro , Lacavalla Maria A. , Malatesta Manuela , Scambi Ilaria , Mariotti Raffaella , Lüdtke-Buzug Kerstin , Corsi Mauro , Marzola Pasquina
Published: September 2024

Adipose tissue-derived adult stem (ADAS) cells and extracellular vesicle (EV) therapy offer promising avenues for treating neurodegenerative diseases due to their accessibility and potential for autologous cell transplantation. However, the clinical application of ADAS cells or EVs is limited by the challenge of precisely identifying them in specific regions of interest. This study compares two superparamagnetic iron oxide nanoparticles, differing mainly in size, to determine their efficacy for allowing non-invasive ADAS tracking via MRI/MPI and indirect labeling of EVs. We compared a USPIO (about 5 nm) with an SPIO (Resovist®, about 70 nm). A physicochemical characterization of nanoparticles was conducted using DLS, TEM, MRI, and MPI. ADAS cells were labeled with the two nanoparticles, and their viability was assessed via MTT assay. MRI detected labeled cells, while TEM and Prussian Blue staining were employed to confirm cell uptake. The results revealed that Resovist® exhibited higher transversal relaxivity value than USPIO and, consequently, allows for detection with higher sensitivity by MRI. A 200 µgFe/mL concentration was identified as optimal for ADAS labeling. MPI detected only Resovist®. The findings suggest that Resovist® may offer enhanced detection of ADAS cells and EVs, making it suitable for multimodal imaging. Preliminary results obtained by extracting EVs from ADAS cells labeled with Resovist® indicate that EVs retain the nanoparticles, paving the way to an efficient and multimodal detection of EVs.
Grover’s Disease Association with Cutaneous Keratinocyte Cancers: More than a Coincidence?
Authors: Nedelcu Roxana , Dobre Alexandra , Turcu Gabriela , Andrei Razvan , Balasescu Elena , Pantelimon Florentina , David-Niculescu Mihaela , Dobritoiu Adina , Radu Raluca , Zaharia Georgiana Roxana , Hulea Ionela , Brinzea Alice , Manea Lorena , Gherghiceanu Mihaela , Ion Daniela
Published: September 2024

Better mechanistic understanding of desmosome disruption and acantholysis in Grover’s disease (GD) may improve management of this disease. Recent molecular studies highlighted promising pathways to be explored by directly comparing GD and selected features of associated skin diseases. The association between GD and cutaneous keratinocyte carcinomas, the most prevalent non-melanoma skin cancers (NMSC), is not completely characterized. To review the medical literature regarding GD-associated cutaneous keratinocyte cancers, focusing on molecular features, pathophysiological mechanisms, and disease associations, to help guide future research and patient management. GD has been associated with a variety of skin conditions, but its association with skin cancers has been rarely reported. Between 1983 and 2024, only nine scientific papers presented data supporting this association. Interestingly, we found that GD may mimic multiple NMSCs, as few authors reported GD cases misdiagnosed as multiple cutaneous squamous cell carcinomas for more than 4 years or the presence of superficial basal cell carcinoma-like areas associated with focal acantholysis. In conclusion: (a) GD may be an imitator of multiple NMSCs, and (b) the relationship between GD and NMSCs may reveal promising pathways for the mechanistic understanding of desmosome disruption and acantholysis in GD and may even lead to its reclassification as a distinctive syndrome.
Establishing Treatment Effectiveness in Fabry Disease: Observation-Based Recommendations for Improvement
Authors: Veldman Bram C. F. , Schoenmakers Daphne H. , van Dussen Laura , Datema Mareen R. , Langeveld Mirjam
Published: September 2024

Fabry disease (FD, OMIM #301500) is caused by pathogenic GLA gene (OMIM #300644) variants, resulting in a deficiency of the α-galactosidase A enzyme with accumulation of its substrate globotriaosylceramide and its derivatives. The phenotype of FD is highly variable, with distinctive disease features and course in classical male patients but more diverse and often nonspecific features in non-classical and female patients. FD-specific therapies have been available for approximately two decades, yet establishing robust evidence for long-term effectiveness remains challenging. This review aims to identify the factors contributing to this lack of robust evidence for the treatment of FD with enzyme replacement therapy (ERT) (agalsidase-alfa and -beta and pegunigalsidase alfa) and chaperone therapy (migalastat). Major factors that have been identified are study population heterogeneity (concerning sex, age, phenotype, disease stage) and differences in study design (control groups, outcomes assessed), as well as the short duration of studies. To address these challenges, we advocate for patient matching to improve control group compatibility in future FD therapy studies. We recommend international collaboration and harmonization, facilitated by an independent FD registry. We propose a stepwise approach for evaluating the effectiveness of novel treatments, including recommendations for surrogate outcomes and required study duration.
Toxicity and Metabolomic Dysfunction Invoked by Febrifugin, a Harmful Component of Edible Nut of Swietenia macrophylla
Authors: Zhang Xiaoyue , Song Qinyang , Zheng Hanghang , Wang Rui , Zhang Qiang
Published: September 2024

Swietenia macrophylla fruit is a valuable and historically significant medicinal plant with anti-hypertension and anti-diabetes. We identified a toxic component, Febrifugin, from the edible part of the nut following zebrafish toxicity-guided isolation. Febrifugin is a mexicanolide-type limonoid compound. The toxic factor induced acute toxicity in zebrafish, including yolk sac edema and pericardial edema, reduced body length, decreased melanin deposition, and presented acute skeletal developmental issues. Further exploration of the acute toxicity mechanism through metabolomics revealed that Febrifugin caused significant changes in 13 metabolites in zebrafish larvae, which are involved in the pentose phosphate, tricarboxylic acid (TCA) cycle, and amino acid biosynthesis. The bioassay of oxidative stress capacity and qRT-PCR measurement showed that the compound significantly affected the h6pd gene in the pentose phosphate pathway and the mRNA expression of cs, idh3a, fh, and shda genes in the TCA cycle, leading to reactive oxygen species (ROS) accumulation and a notable decrease in glutathione (GSH) activity in zebrafish. These findings provide a basis for the rational use of S. macrophylla as a medicinal plant and raise awareness of the safety of medicinal plants.
GWAS Enhances Genomic Prediction Accuracy of Caviar Yield, Caviar Color and Body Weight Traits in Sturgeons Using Whole-Genome Sequencing Data
Authors: Song Hailiang , Dong Tian , Wang Wei , Yan Xiaoyu , Geng Chenfan , Bai Song , Hu Hongxia
Published: September 2024

Caviar yield, caviar color, and body weight are crucial economic traits in sturgeon breeding. Understanding the molecular mechanisms behind these traits is essential for their genetic improvement. In this study, we performed whole-genome sequencing on 673 Russian sturgeons, renowned for their high-quality caviar. With an average sequencing depth of 13.69×, we obtained approximately 10.41 million high-quality single nucleotide polymorphisms (SNPs). Using a genome-wide association study (GWAS) with a single-marker regression model, we identified SNPs and genes associated with these traits. Our findings revealed several candidate genes for each trait: caviar yield: TFAP2A, RPS6KA3, CRB3, TUBB, H2AFX, morc3, BAG1, RANBP2, PLA2G1B, and NYAP1; caviar color: NFX1, OTULIN, SRFBP1, PLEK, INHBA, and NARS; body weight: ACVR1, HTR4, fmnl2, INSIG2, GPD2, ACVR1C, TANC1, KCNH7, SLC16A13, XKR4, GALR2, RPL39, ACVR2A, ADCY10, and ZEB2. Additionally, using the genomic feature BLUP (GFBLUP) method, which combines linkage disequilibrium (LD) pruning markers with GWAS prior information, we improved genomic prediction accuracy by 2%, 1.9%, and 3.1% for caviar yield, caviar color, and body weight traits, respectively, compared to the GBLUP method. In conclusion, this study enhances our understanding of the genetic mechanisms underlying caviar yield, caviar color, and body weight traits in sturgeons, providing opportunities for genetic improvement of these traits through genomic selection.
Examining the Integrity of Apple’s Privacy Labels: GDPR Compliance and Unnecessary Data Collection in iOS Apps
Authors: Surma Zaid Ahmad , Gowdar Saiesha , Pandit Harshvardhan J.
Published: September 2024

This study investigates the effectiveness of Apple’s privacy labels, introduced in iOS 14, in promoting transparency around app data collection practices with respect to the GDPR. Specifically, we address two key research questions: (1) What special categories of personal data, as regulated by the GDPR, are collected and used by apps, and for which purposes? (2) What disparities exist between app-stated permissions and the apparent unnecessary data gathering across various categories in the iOS App Store? By analyzing a comprehensive dataset of 541,662 iOS apps, we identify common practices related to prevalent use of sensitive and special categories of personal data, revealing widespread instances of unnecessary data collection, misuse, and potential GDPR violations. Furthermore, our analysis uncovers significant inconsistencies between the permissions stated by apps and the actual data they gather, highlighting a critical gap in user privacy protection within the iOS ecosystem. These findings underscore the need for stricter regulatory oversight of app stores and the necessity of effective privacy notices to build accountability and trust and ensure transparency. This study offers actionable insights for regulators, app developers, and users towards creating secure and transparent digital ecosystems.