bluuebunny/crossref_metadata_2025 huggingface dataset:
Beyond Magic: Fostering Literacy Resilience in Diverse Classrooms through Home-Based Approaches
Authors: Eliyahu-Levi Dolly
Published: September 2024

The classrooms in Israel are very diverse, with students differing in learning styles, their handling of literacy tasks, personal and socioeconomic backgrounds, and more. These differences significantly impact the curriculum aimed at promoting literacy resilience, explicit teaching processes in the classroom, and imparting metacognitive strategies and actions to overcome learning difficulties. This qualitative-interpretative study reveals the pedagogical perceptions, challenges, and coping strategies of fourteen Hebrew teachers in five elementary schools in central Israel regarding integrating home literacy in language lessons and cultivating literacy resilience among their students. The research data were collected through in-depth interviews with the teachers. The analysis of the teachers’ reports reveals two main perceptions regarding literacy resilience: (1) Literacy resilience is a tool for life; (2) Home literacy significantly contributes to fostering literacy resilience. Furthermore, cultivating literacy resilience presents three significant challenges for the teachers: (1) Teaching in a heterogeneous classroom, (2) Encouraging parental involvement, and (3) Fostering independent learners. To cultivate literacy resilience in a heterogeneous classroom, teachers must be sensitive to each student’s unique needs and plan teaching-learning processes based on principles of self-directed learning and peer dialogue. They must establish a personal-emotional connection that is a significant anchor for the students and outlines a path for integrating and strengthening the sense of competence in handling literacy tasks. It was also found that parental involvement is a significant factor influencing the cultivation of literacy resilience, and teachers undertake various actions to increase their level of involvement. This study adds an essential layer to the body of knowledge regarding the understanding of the factors affecting the development of pedagogical literacy perceptions that promote the integration of home literacy in the classroom. These perceptions may promote the nurturing process of literacy resilience among students from various cultures, accepting and understanding them. In this way, we can attempt to address literacy and language challenges in Israel.
The Interplay of Structuring and Controlling Teaching Styles in Physical Education and Its Impact on Students’ Motivation and Engagement
Authors: Coterón Javier , Fernández-Caballero José , Martín-Hoz Laura , Franco Evelia
Published: September 2024

Background: Teaching style has a significant influence on students’ learning outcomes. This study focused on identifying teaching profiles in Physical Education characterized by high directiveness, using structure and control behaviors that impact students’ outcomes, basic psychological needs (BPN), and engagement. It was based on the circumplex model and self-determination theory (SDT) and intended to explore how these styles affect students’ motivation and engagement. Methods: A cluster-based methodological design was applied, evaluating teachers through self-reports. Adapted measures of structure and control were used to classify teachers into four distinct profiles within the educational context of Physical Education. Results: The study identified three teaching profiles: ‘high structure–low control’, ‘high structure–high control’, ‘low structure–low control’, and ‘low structure–high control’. The ‘high structure–low control’ profile showed the best results in autonomous and controlled motivation, with greater behavioral engagement among students. In contrast, the ‘high structure–high control’ profile was associated with higher levels of demotivation. Conclusions: Teaching styles of structure and control can combine in various ways among Physical Education teachers, significantly influencing student motivation, satisfaction of basic psychological needs, and engagement. It is recommended that teachers adopt behaviors that support structure without becoming controlling to enhance student learning and participation in classes.
Comparison of Deterministic, Stochastic, and Energy-Data-Driven Occupancy Models for Building Stock Energy Simulation
Authors: Al-Saegh Salam , Tahmasebi Farhang , Tang Rui , Mumovic Dejan
Published: September 2024

Accurate modelling of occupancy patterns is critical for reliable estimation of building stock energy demand, which is a key input for the design of district energy systems. Aiming to investigate the suitability of different occupancy-modelling approaches for the design of district energy systems, the present study examines a set of standard-based schedules (from the UK National Calculation Methodology), a widely used stochastic occupancy model, and a novel energy-data-driven occupancy model. To this end, a dynamic energy model of a higher education office building developed within a stock model of London’s Bloomsbury district serves as a testbed to implement the occupancy models, explore their implications for the estimation of annual and peak heating and cooling demand, and extrapolate the findings to the computationally demanding building stock stimulations. Furthermore, the simulations were conducted in two years before and after the COVID-19 pandemic to examine the implications of hybrid working patterns after the pandemic. From the results, the energy-data-driven model demonstrated superior performance in annual heating demand estimations, with errors of ±2.5% compared to 14% and 7% for the standard-based and stochastic models. For peak heating demand, the models performed rather similarly, with the data-driven model showing 28% error compared to 29.5% for both the standard-based and stochastic models in 2019. In cooling demand estimations, the data-driven model yielded noticeably higher annual cooling demand and lower peak cooling demand estimations as compared with the standard-based and stochastic occupancy models. Given the adopted building-modelling approach, these findings can be extended to district-level investigations and inform the decision on the choice of occupancy models for building stock energy simulation.
Mechanical Properties of Permeable Concrete Reinforced with Polypropylene Fibers for Different Water–Cement Ratios
Authors: Ramírez Wladimir , Mayacela Margarita , Contreras Luis , Shambi Alejandra , Ramírez Francisco , Chacón Jonatan
Published: September 2024

Permeable concrete is a material that allows water filtration, reduces surface runoff, and maintains the natural water cycle. Previous studies have shown that its mechanical properties, particularly its compressive and flexural tensile strengths, are generally lower than those of conventional concrete, with significant variability observed among similar tests. This study investigates the compressive strength, flexural strength, and permeability of polypropylene fiber-reinforced permeable concrete specimens at two water–cement ratios (0.30 and 0.35). The mix design was conducted using the ACI 522R-10 method. Forty-eight cylinders measuring 200 mm × 100 mm were fabricated for permeability and compression tests. Additionally, 12 beams measuring 100 mm × 100 mm × 350 mm were produced and subjected to simple flexural testing in accordance with ASTM C78 guidelines. Compressive strength versus permeability and load versus deflection graphs were plotted, and the fracture energy was calculated for various deflections. The results indicate that the addition of fibers increased permeability and tensile strength but decreased compressive strength. Furthermore, an increase in the water–cement ratio led to higher compressive and flexural tensile strengths.
Use of Ceramic Tile Wastes as Raw Substitution Material in the Production of Blended Cement
Authors: Baylavlı Hasan , Gödek Eren
Published: September 2024

In this study, the feasibility of using ceramic wastes in the production of blended cement was evaluated by substituting limestone with ceramic waste at the percentages of 5, 10, 15, 20, and 28% before the milling stage. The chemical, physical, and mechanical properties of the cements were determined according to relevant standards, and the results were compared. The chemical analysis showed that the SiO2 content of the cements increased with higher ceramic waste substitution percentages, while the CaCO3 content decreased. The grindability of cements decreased with increased ceramic waste ratios, slightly reducing the Blaine specific surface area values. The water consistency for the cements was set at 28%, and all the cements met the standard limitations. The spread diameters for all types of cements were similar and practically usable in terms of workability. The cements containing ceramic waste either maintained or extended the setting time. All cements with ceramic waste exhibited higher flexural and compressive strength compared to the reference cement. The highest flexural strengths were achieved with a 28% ceramic waste substitution ratio across all curing ages. Regarding compressive strengths, all cements exhibited higher compressive strength than 10 MPa at 2 days and 32.5 MPa at 28 days, classifying them as 32.5 R-type blended cements. When the medium- (56–90 days) and long-term (365 days) compressive strengths were compared, the highest strength values were obtained from the cement with a 28% ceramic waste substitution. Although limestone-blended cement is emerging as a promising alternative to traditional Portland cement, these types of cement still contribute to environmental degradation due to the extraction of natural limestone resources through quarrying. This study showed that blended cements can be produced using ceramic waste, providing a more sustainable and environmentally friendly solution for the construction industry.
Multidimensional Seismic Fragility Study of Intake Towers Based on Incremental Dynamic Analysis
Authors: Li Xiaona , Zhou Yingjie , Zhu Hemin , Li Yuchen , An Haowen
Published: September 2024

Assessing the fragility of intake towers using a single damage index does not allow for accurate evaluation of the potential for structural damage under seismic conditions. In this study, based on the probabilistic seismic demand analysis method, the effects of ground motion intensity on maximum displacement, local damage index, and global damage index are considered, and the seismic fragility of an intake tower structure is analyzed. First, 10 natural ground motion records were selected from the ground motion database (PEER) and 2 artificial seismic waves were synthesized. These seismic waves were amplitude-modulated for incremental dynamic analysis (IDA). The trends of the IDA curves were analyzed to divide the performance levels of the intake tower structure. Furthermore, a two-dimensional fragility curve for the intake tower structure was plotted in this study. The maximum displacement in the direction of parallel flow and the damage index were taken into account in the two-dimensional fragility curve. The results show that, under the designed seismic acceleration, the two-dimensional fragility curve for the intake tower structure was lower than the one-dimensional curve. This indicates that the seismic design based on the one-dimensional performance index was unstable. This provides a theoretical reference for seismic optimization design and the strengthening of intake towers. Therefore, it is recommended to use multidimensional fragility analysis to study the seismic performance of intake tower structures in seismic design.
Research on Child-Friendly Evaluation and Optimization Strategies for Rural Public Spaces
Authors: Fan Jia , Zheng Bohong , Liu Junyou , Tian Fangzhou , Sun Zhaoqian
Published: September 2024

Public spaces are essential for the implementation of child-friendly principles and the development of child-friendly cities, with positive and healthy environments playing a crucial role in supporting children’s well-being and development. However, existing research on child-friendly public spaces predominantly targets economically developed urban areas with robust public service infrastructure, often neglecting rural areas with less advanced facilities. This study utilizes grounded theory and qualitative analysis to propose a child-friendly public space evaluation framework specifically for rural settings. The framework includes four primary indicators—safety, accessibility, comfort, and multifunctionality—and 19 secondary indicators, such as facility safety and plant safety. An empirical investigation was conducted in Baishoupu Town, a child-friendly pilot area within Changsha, China, which is designated as a United Nations Child-Friendly City, and the study encompassed an analysis of 11 rural villages within this area. The findings reveal that while Baishoupu Town demonstrates a relatively high level of child-friendly development, there is significant disparity among individual villages. Key determinants affecting the child-friendliness of rural public spaces include the type of rural industry, per capita income levels, and the degree of policy support. Specifically, the advancement of public service infrastructure and the tourism sector significantly influence the primary indicators. Moreover, while rural road infrastructure is positively correlated with accessibility, the presence of through traffic adversely affects safety indicators. Based on these insights, this study recommends enhancing child-friendliness in rural public spaces through strategic village planning, spatial design improvements, and ensuring child participation. This research provides valuable insights for government policy development and implementation and offers a replicable framework for child-friendly public space development in rural areas globally.
Enhanced Antibacterial Activity of Carbon Dots: A Hybrid Approach with Levofloxacin, Curcumin, and Tea Polyphenols
Authors: Abbas Khurram , Zhu Haimei , Qin Weixia , Wang Meiyan , Li Zijian , Bi Hong
Published: September 2024

Bacterial infections and their increasing resistance to antibiotics pose a significant challenge in medical treatment. This study presents the synthesis and characterization of novel carbon dots (CDs) using levofloxacin (Lf), curcumin (Cur), and tea polyphenols (TP) through a facile hydrothermal method. The synthesized curcumin-tea polyphenol@carbon dots (Cur-TP@CDs) and levofloxacin-tea polyphenol@carbon dots (Lf-TP@CDs) were characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy, confirming their unique structural and chemical properties. Cur-TP@CDs exhibited an average particle size of 1.32 nanometers (nm), while Lf-TP@CDs averaged 1.58 nm. Both types demonstrated significant antibacterial activity, with Lf-TP@CDs showing superior effectiveness against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) in broth dilution and disc diffusion assays. Biofilm inhibition assays revealed a significant reduction in biofilm formation at higher concentrations. The ultraviolet-visible (UV-vis) and photoluminescence (PL) spectral analyses indicated efficient photon emission, and electron paramagnetic resonance (EPR) analysis showed increased singlet oxygen generation, enhancing bactericidal effects. Live and dead bacterial staining followed by scanning electron microscopy (SEM) analysis confirmed dose-dependent bacterial cell damage and morphological deformities. These findings suggest that Cur-TP@CDs and Lf-TP@CDs are promising antibacterial agents, potentially offering a novel approach to combat antibiotic-resistant bacterial infections.
Unraveling the Regulatory Role of HuR/microRNA Axis in Colorectal Cancer Tumorigenesis
Authors: Yadav Vikas , Singh Tejveer , Sharma Deepika , Garg Vivek Kumar , Chakraborty Payel , Ghatak Souvik , Satapathy Shakti Ranjan
Published: September 2024

Colorectal cancer (CRC) remains a significant global health burden with high incidence and mortality. MicroRNAs (miRNAs) are small non-protein coding transcripts, conserved throughout evolution, with an important role in CRC tumorigenesis, and are either upregulated or downregulated in various cancers. RNA-binding proteins (RBPs) are known as essential regulators of miRNA activity. Human antigen R (HuR) is a prominent RBP known to drive tumorigenesis with a pivotal role in CRC. In this review, we discuss the regulatory role of the HuR/miRNA axis in CRC. Interestingly, miRNAs can directly target HuR, altering its expression and activity. However, HuR can also stabilize or degrade miRNAs, forming complex feedback loops that either activate or block CRC-associated signaling pathways. Dysregulation of the HuR/miRNA axis contributes to CRC initiation and progression. Additionally, HuR-miRNA regulation by other small non-coding RNAs, circular RNA (circRNAs), or long-non-coding RNAs (lncRNAs) is also explored here. Understanding this HuR-miRNA interplay could reveal novel biomarkers with better diagnostic or prognostic accuracy.
Syngeneic Mouse Models for Pre-Clinical Evaluation of CAR T Cells
Authors: Ahmed Eman N. , Cutmore Lauren C. , Marshall John F.
Published: September 2024

Chimeric antigen receptor (CAR) T cells have revolutionized the treatment of hematological malignancies. Unfortunately, this improvement has yet to be translated into the solid tumor field. Current immunodeficient models used in pre-clinical testing often overestimate the efficacy of CAR T cell therapy as they fail to recapitulate the immunosuppressive tumor microenvironment characteristic of solid tumors. As CAR T cell monotherapy is unlikely to be curative for many solid tumors, combination therapies must be investigated, for example, stromal remodeling agents and immunomodulators. The evaluation of these combination therapies requires a fully immunocompetent mouse model in order to recapitulate the interaction between the host’s immune system and the CAR T cells. This review will discuss the need for improved immunocompetent murine models for the pre-clinical evaluation of CAR T cells, the current use of such models and future directions.
Transition Metal-Promoted LDH-Derived CoCeMgAlO Mixed Oxides as Active Catalysts for Methane Total Oxidation
Authors: Stoian Marius C. , Romanitan Cosmin , Neubauer Katja , Atia Hanan , Negrilă Constantin Cătălin , Popescu Ionel , Marcu Ioan-Cezar
Published: September 2024

A series of M(x)CoCeMgAlO mixed oxides with different transition metals (M = Cu, Fe, Mn, and Ni) with an M content x = 3 at. %, and another series of Fe(x)CoCeMgAlO mixed oxides with Fe contents x ranging from 1 to 9 at. % with respect to cations, while keeping constant in both cases 40 at. % Co, 10 at. % Ce and Mg/Al atomic ratio of 3 were prepared via thermal decomposition at 750 °C in air of their corresponding layered double hydroxide (LDH) precursors obtained by coprecipitation. They were tested in a fixed bed reactor for complete methane oxidation with a gas feed of 1 vol.% methane in air to evaluate their catalytic performance. The physico-structural properties of the mixed oxide samples were investigated with several techniques, such as powder X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDX), elemental mappings, inductively coupled plasma optical emission spectroscopy (ICP-OES), X-ray photoelectron spectroscopy (XPS), temperature-programmed reduction under hydrogen (H2-TPR) and nitrogen adsorption–desorption at −196 °C. XRD analysis revealed in all the samples the presence of Co3O4 crystallites together with periclase-like and CeO2 phases, with no separate M-based oxide phase. All the cations were distributed homogeneously, as suggested by EDX measurements and elemental mappings of the samples. The metal contents, determined by EDX and ICP-OES, were in accordance with the theoretical values set for the catalysts’ preparation. The redox properties studied by H2-TPR, along with the surface composition determined by XPS, provided information to elucidate the catalytic combustion properties of the studied mixed oxide materials. The methane combustion tests showed that all the M-promoted CoCeMgAlO mixed oxides were more active than the M-free counterpart, the highest promoting effect being observed for Fe as the doping transition metal. The Fe(x)CoCeMgAlO mixed oxide sample, with x = 3 at. % Fe displayed the highest catalytic activity for methane combustion with a temperature corresponding to 50% methane conversion, T50, of 489 °C, which is ca. 40 °C lower than that of the unpromoted catalyst. This was attributed to its superior redox properties and lowest activation energy among the studied catalysts, likely due to a Fe–Co–Ce synergistic interaction. In addition, long-term tests of Fe(3)CoCeMgAlO mixed oxide were performed, showing good stability over 60 h on-stream. On the other hand, the addition of water vapors in the feed led to textural and structural changes in the Fe(3)CoCeMgAlO system, affecting its catalytic performance in methane complete oxidation. At the same time, the catalyst showed relatively good recovery of its catalytic activity as soon as the water vapors were removed from the feed.
Carbon Nanofiber-Encapsulated FeCoNiCuMn Sulfides with Tunable S Doping for Enhanced Oxygen Evolution Reaction
Authors: Sun Yuhan , Shen Chen , Wang Mingran , Cao Yang , Wang Qianwei , Rong Jiayi , He Tong , Li Duanyang , Cao Feng
Published: September 2024

The oxygen evolution reaction (OER) stands out as a key electrochemical process for the conversion of clean energy. However, the practical implementation of OER is frequently impeded by its slow kinetics and the necessity for scarce and expensive noble metal catalysts. High-entropy transition metal sulfides (HETMS) stand at the forefront of OER catalysts, renowned for their exceptional catalytic performance and diversity. Herein, we have synthesized a HETMS catalyst, (FeCoNiCuMn50)S2, encapsulated within carbon nanofibers through a one-step process involving the synergistic application of electrospinning and chemical vapor deposition. By precisely controlling the doping levels of sulfur, we have demonstrated that sulfur incorporation significantly increases the exposed surface area of alloy particles on carbon nanofibers and optimizes the electronic configuration of the alloy elements. These findings reveal that sulfur doping is instrumental in the substantial improvement of the catalyst’s OER performance. Notably, the catalyst showed optimal activity at a sulfur-to-metal atom ratio of 2:1, delivering an overpotential of 254 mV at a current density of 10 mA cm−2 in 1.0 M KOH solution. Furthermore, the (FeCoNiCuMn50)S2 catalyst exhibited remarkable electrochemical stability, underscoring its potential as an efficient and robust OER electrocatalyst for sustainable energy applications.
Tuning the Electronic Structures of Mo-Based Sulfides/Selenides with Biomass-Derived Carbon for Hydrogen Evolution Reaction and Sodium-Ion Batteries
Authors: Pan Hongying , Zheng Kaiyang , Chen Zihan , Wang Yuexin , Tan Yajun , Wang Jian , Yao Luye , Wang Lixin , Sun Chencheng , Yang Jun
Published: September 2024

A key research focus at present is the exploration and innovation of electrode materials suitable for energy storage and conversion. Molybdenum-based sulfides/selenides (primarily MoS2 and MoSe2) have garnered attention in recent years due to their intrinsic two-dimensional structures, which are conducive to ion/electron transfer or insertion/extraction, making them promising candidates in electrocatalytic hydrogen production and sodium-ion battery applications. However, their inherently poor electronic structures have led most research efforts to concentrate on modifications aimed at enhancing their performance in hydrogen evolution reactions (HERs) and sodium-ion batteries (SIBs). Owing to their remarkable chemical inertness, expansive specific surface areas, and tunable pore architectures, carbon-based materials have garnered significant attention in research. The utilization of biomass as a renewable and environmentally sustainable precursor offers considerable benefits, including abundant availability, ecological compatibility, and cost-effectiveness. Consequently, recent scholarly endeavors have concentrated intensively on the synthesis of valuable carbon materials derived from renewable biomass sources. This review addresses the scientific challenges related to the development of electrode materials for HERs and SIBs in electrochemical energy storage and conversion. It delves into the recent focus on the two-dimensional transition-metal chalcogenides, particularly MoS2 and MoSe2, and the difficulties encountered in modulating their electronic structures when applied to HERs and SIBs. The review proposes the use of eco-friendly and widely sourced biomass-derived carbon (BMC) as a supporting matrix combined with MoS2 and MoSe2 to regulate their structures and enhance their electrocatalytic activity and sodium storage performance. Additionally, it highlights the existing challenges faced by these BMC/MoS2 and BMC/MoSe2 composites and offers insights into future developments.
Novel Approach to Organization of Structured Cobalt-Based Fischer–Tropsch Catalyst
Authors: Gorshkov Andrei , Sineva Lilia , Gryaznov Kirill , Mordkovich Vladimir
Published: September 2024

Structured Fischer–Tropsch synthesis catalysts were tested in tubular reactors of industry-standard diameters of 0.5 or 0.75 inches. The structured catalyst bed was manufactured by the obturation of a straight bunch of graphite-based extrudates (D = 1.5 mm, L = 30 mm). A conventional loose bed of granulated catalyst (D = 1.5 mm, L = 3 mm) was tested as a reference. In a 1000–3000 h−1 syngas space velocity range, structured and loose catalyst bed testing showed no significant differences in their main catalytic parameters. Nevertheless, their C5+ hydrocarbon group composition was quite different, i.e., the alkene fraction rose from 9 to 23%, while n-alkanes dropped from 81 to 64%. This could be a result of secondary reaction intensification in the conventional loose bed due to its zeolite acid site’s higher availability. Further FTS testing of the structured catalysts in 4000–6000 h−1 manifested distinctive limits in C5+ productivity for 0.5 and 0.75 inches of 512 kg C5+/(m3 reactor·h) and 362 kg C5+/(m3 reactor·h), respectively. This may be explained by limitations in structured bed thermal conductivity. It suggests that the arrangement of extrudates in the structured catalyst can significantly affect the reaction heat and mass transfer conditions and affords new opportunities for group composition control by means of catalyst bed organization.
Production of Bio-Oil from Sugarcane Bagasse through Hydrothermal Liquefaction Processes with Modified Zeolite Socony Mobil-5 Catalyst
Authors: Jideani Thandiswa , Seroka Ntalane Sello , Khotseng Lindiwe
Published: September 2024

In response to the increasing global demand for sustainable energy alternatives, this research explores the efficient conversion of sugarcane bagasse to bio-oil through hydrothermal liquefaction (HTL) processes with modified Zeolite Socony Mobil-5 catalysts (ZSM-5). The study systematically investigates the impact of feedstock quantity, reaction temperature, duration, and catalyst loading on bio-oil yield and quality. Optimisation experiments revealed that a feedstock amount of 10 grammes, an HTL temperature of 340 °C for 60 min and a ZSM-5 catalyst loading of 3 grammes resulted in the highest bio-oil yield. Furthermore, the introduction of Ni and Fe metals to ZSM-5 exhibited enhanced catalytic activity without compromising the structure of the zeolites. Comprehensive characterisation of modified catalysts using SEM-EDS, XRD, TGA, TEM, and FTIR provided insight into their structural and chemical properties. The successful incorporation of Ni and Fe into ZSM-5 was confirmed, highlighting promising applications in hydrothermal liquefaction. Gas chromatography–mass spectrometry (GC-MS) analysis of bio-oils demonstrated the effectiveness of the 2% Fe/ZSM-5 catalyst, highlighting a significant increase in hydrocarbon content. FTIR analysis of the produced bio-oils indicated a reduction in functional groups and intensified aromatic peaks, suggesting a shift in chemical composition favouring aromatic hydrocarbons. This study provides valuable information on HTL optimisation, catalyst modification, and bio-oil characterisation, advancing the understanding of sustainable biofuel production. The findings underscore the catalytic prowess of modified ZSM-5, particularly with iron incorporation, in promoting the formation of valuable hydrocarbons during hydrothermal liquefaction.
Enhancement Study of the Photoactivity of TiO2 Photocatalysts during the Increase of the WO3 Ratio in the Presence of Ag Metal
Authors: Aldirham Sharah H. , Helal Ahmed , Shkir Mohd , Sayed M. A. , Ali Atif Mossad
Published: September 2024

Nanocomposites (NCs) consisting of 4%Ag/x%WO3/TiO2, with varied concentrations (x = 1, 3, 5, 7 wt.%) of WO3, were successfully synthesized using the sol-gel process to examine their photocatalytic performance. The synthesized 4%Ag/x%WO3/TiO2 nanopowder was characterized using X-ray diffraction (XRD), Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–vis diffuse reflectance spectra (UV–vis DRS), photoluminescence (PL), and Brunauer–Emmett–Teller (BET) surface area analysis to elucidate its physicochemical properties. The photocatalytic evaluation revealed that the Ag/1%WO3/TiO2 nanocomposite exhibits 98% photoreduction efficiency for Cr(VI) after 2 h under visible light due to the impact of the plasmonic effect of Ag atoms. In addition, the Ag/4%WO3/TiO2 shows about 95% photooxidation efficiency for methylene blue (MB) dye after 4 h.
Single-Cell Transcriptomics Applied in Plants
Authors: Sun Yanyan , Sun Jian , Lin Chunjing , Zhang Jingyong , Yan Hao , Guan Zheyun , Zhang Chunbao
Published: September 2024

Single-cell RNA sequencing (scRNA-seq) is a high-tech method for characterizing the expression patterns of heterogeneous cells in the same tissue and has changed our evaluation of biological systems by increasing the number of individual cells analyzed. However, the full potential of scRNA-seq, particularly in plant science, has not yet been elucidated. To explore the utilization of scRNA-seq technology in plants, we firstly conducted a comprehensive review of significant scRNA-seq findings in the past few years. Secondly, we introduced the research and applications of scRNA-seq technology to plant tissues in recent years, primarily focusing on model plants, crops, and wood. We then offered five databases that could facilitate the identification of distinct expression marker genes for various cell types. Finally, we analyzed the potential problems, challenges, and directions for applying scRNA-seq in plants, with the aim of providing a theoretical foundation for the better use of this technique in future plant research.
SIK2 Controls the Homeostatic Character of the POMC Secretome Acutely in Response to Pharmacological ER Stress Induction
Authors: Türküner Mehmet , Yazıcı Ayşe , Özcan Ferruh
Published: September 2024

The neuronal etiology of obesity is centered around a diet-induced inflammatory state in the arcuate nucleus of the hypothalamus, which impairs the functionality of pro-opiomelanocortine neurons (POMCs) responsible for whole-body energy homeostasis and feeding behavior. Intriguingly, systemic salt inducible kinase 2 (SIK2) knockout mice demonstrated reduced food intake and energy expenditure along with modestly dysregulated metabolic parameters, suggesting a causal link between the absence of SIK2 activity in POMCs and the observed phenotype. To test this hypothesis, we conducted a comparative secretomics study from POMC neurons following pharmacologically induced endoplasmic reticulum (ER) stress induction, a hallmark of metabolic inflammation and POMC dysregulation in diet-induced obese (DIO) mice. Our data provide significant in vitro evidence for the POMC-specific SIK2 activity in controlling energy metabolism and feeding in DIO mice by regulating the nature of the related POMC secretome. Our data also suggest that under physiological stress conditions, SIK2 may act as a gatekeeper for the secreted inflammatory factors and signaling molecules critical for cellular survival and energy homeostasis. On the other hand, in the absence of SIK2, the gate opens, leading to a surge of inflammatory cytokines and apoptotic cues concomitant with the dysregulation of POMC neurons.
A Novel Recombinant Vitronectin Variant Supports the Expansion and Differentiation of Pluripotent Stem Cells in Defined Animal-Free Workflows
Authors: Lu Xi , Perr Eli , Naqvi Tahmina , Galitz David , Andersen Marnelle , Grabowski David , Person Anthony , Kalyuzhny Alex , Flynn Kevin C.
Published: September 2024

An essential aspect of harnessing the potential of pluripotent stem cells (PSCs) and their derivatives for regenerative medicine is the development of animal-free and chemically defined conditions for ex vivo cultivation. PSCs, including embryonic and induced PSCs (iPSCs), are in the early stages of clinical trials for various indications, including degenerative diseases and traumatic injury. A key step in the workflows generating these cells for more widespread clinical use is their safe and robust ex vivo cultivation. This entails optimization of cell culture media and substrates that are safe and consistent while maintaining robust functionality. Here, we describe the design of a human vitronectin (hVTN) variant with improved manufacturability in a bacterial expression system along with improved function in comparison to wild-type VTN and other previously characterized polypeptide fragments. In conjunction with an animal component-free media formulation, our hVTN fragment provides animal-free conditions for the enhanced expansion of iPSCs. This hVTN variant also supports the reprogramming of PBMCs into iPSCs. Furthermore, we show that these iPSCs can be efficiently differentiated into the three major germ layers and cortical neurons, thereby closing the loop on a completely defined animal-free workflow for cell types relevant for regenerative medicine.
Evaluation of Anti-Inflammatory Activity of the New Cardiotonic Steroid γ-Benzylidene Digoxin 8 (BD-8) in Mice
Authors: Ferreira Davi Azevedo , Medeiros Anna Beatriz Araujo , Soares Mariana Mendonça , Lima Éssia de Almeida , Oliveira Gabriela Carolina Santos Lima de , Leite Mateus Bernardo da Silva , Machado Matheus Vieira , Villar José Augusto Ferreira Perez , Barbosa Leandro Augusto , Scavone Cristoforo , Moura Marcelo Tigre , Rodrigues-Mascarenhas Sandra
Published: September 2024

Cardiotonic steroids are known to bind to Na+/K+-ATPase and regulate several biological processes, including the immune response. The synthetic cardiotonic steroid γ-Benzylidene Digoxin 8 (BD-8) is emerging as a promising immunomodulatory molecule, although it has remained largely unexplored. Therefore, we tested the immunomodulatory potential of BD-8 both in vitro and in vivo. Hence, primary mouse macrophages were incubated with combinations of BD-8 and the pro-inflammatory fungal protein zymosan (ZYM). Nitric oxide (NO) production was determined by Griess reagent and cytokines production was assessed by enzyme-linked immunosorbent assay. Inducible nitric oxide synthase (iNOS), reactive oxygen species (ROS), p-nuclear factor kappa B p65 (NF-κB p65), p-extracellular signal-regulated kinase (p-ERK), and p-p38 were evaluated by flow cytometry. Macrophages exposed to BD-8 displayed reduced phagocytic activity, NO levels, and production of the proinflammatory cytokine IL-1β induced by ZYM. Furthermore, BD-8 diminished the expression of iNOS and phosphorylation of NF-κB p65, ERK, and p38. Additionally, BD-8 exhibited anti-inflammatory capacity in vivo in a carrageenan-induced mouse paw edema model. Taken together, these findings demonstrate the anti-inflammatory activity of BD-8 and further reinforce the potential of cardiotonic steroids and their derivatives as immunomodulatory molecules.
The Effect of a Dual Cure Activator on Self-Adhesive Resin Cements and Zirconia Shear Bond Strength
Authors: Akrawatcharawittaya Phalat , Sriamporn Tool , Vuddhakanok Suchada , Thamrongananskul Niyom , Klaisiri Awiruth
Published: September 2024

This study examines the impact of a dual cure activator (DCA) when applied in combination with self-adhesive resin cements on the zirconia. Sixty zirconia were prepared in compliance with the manufacturer’s directions. The specimens were randomly assigned to each group under the dark condition, following DCA and self-adhesive resin cements [RelyX universal resin cement (RXS); Maxcem elite chroma (MAC); Panavia SA cement multi (PSM)]; group 1, RXS; group 2, MAC; group 3, PSM; group 4, DCA + RXS; group 5, DCA + MAC; and group 6, DCA + PSM. The resin composite was fixed to the zirconia, surface-treated, and maintained in a dark container for 30 min. The specimens were kept in an incubator at a temperature of 37 degrees Celsius for 24 h. The universal testing device was employed to compute the shear bond strength (SBS). A stereomicroscope was used to analyze the fractured types. The data were analyzed employing the one-way ANOVA and Tukey’s test. Group 2 had the lowest SBS (4.93 ± 0.53 MPa). Group 1 (11.17 ± 0.86 MPa) and group 3 (11.48 ± 1.17 MPa) were not significantly different in SBS. Group 6 (15.61 ± 0.68 MPa) had the highest SBS but was not significantly different from group 4 (15.45 ± 1.20 MPa). The findings show that treating the zirconia surface with DCA before using the self-curing mode of self-adhesive resin cements is the best way to improve the bond between the zirconia and resin cement.
Changes in Protein Levels during the Storage and Warming of Breast Milk in a Domestic Environment
Authors: Siviroj Penprapa , Ruangsuriya Jetsada , Ongprasert Krongporn
Published: September 2024

Background/Objectives: Storage and warming can impact the protein concentration of breast milk (BM). This study aimed to explore the effects of routine BM handling, from storage to warming, on BM protein concentration. Methods: Forty BM samples were collected from 40 mothers with full-term infants. Fresh BM samples were analyzed within 24 h of expression as a baseline. Additional samples were stored in a refrigerator for up to 4 days and in either a freezer compartment of a refrigerator with a separate door (refrigerator freezers) or a deep freezer for up to 6 months. We compared four warming techniques: rapid thawing in water at 25 °C and 37 °C immediately after freezer removal, and slow thawing in a refrigerator for 12 h, followed by warming in water at 25 °C and 37 °C. The protein concentration was measured using the Lowry method. Results: No significant decrease in BM protein concentration was observed across all storage conditions compared to baseline. BM with a higher protein content benefited more from storage in deep freezers than in refrigerator freezers. Thawing immediately after removal from the freezer at 25 °C preserved significantly higher total protein levels compared to alternative heating techniques. Conclusions: Our findings support the recommendation for mothers to store BM in either type of freezer for up to 6 months. Our results suggest that rapid thawing at 25 °C is the most effective method for preserving protein levels compared to other thawing techniques used in our study.
Elevated Temperature Tribological Behavior of Duplex Layer CrN/DLC and Nano Multilayer DLC-W Coatings Deposited on Carburized and Hardened 16MnCr5 Steel
Authors: Kolawole Funsho Olaitan , Kolawole Shola Kolade , Fukumasu Newton Kiyoshi , Varela Luis Bernardo , Vencovsky Paulo Konrad , Ludewigs Danilo Assad , de Souza Roberto Martins , Tschiptschin André Paulo
Published: September 2024

This study investigates the impact of temperature on the tribological performance of duplex layer CrN/DLC and nano-multilayers DLC-W coatings deposited using hybrid PVD-PECVD techniques on carburized and hardened 16MnCr5 discs cut from internal combustion engines valve tappets. Reciprocating dry sliding experiments were conducted at 25 °C, 150 °C, 200 °C, and 250 °C to analyze the high-temperature tribological behavior of the coatings. The wear mechanisms were characterized using SEM, EDS mapping, Raman spectroscopy, and nanoindentation. The lowest coefficient of friction was obtained for CrN/DLC at 25 °C. The CrN/DLC coefficients of friction (COF) increase with temperatures due to increasing adhesive wear. Similarly, DLC-W exhibited a comparable trend with increasing temperature from 25 °C to 250 °C. Both coatings’ wear resistance decreased with higher temperatures due to the transformation of sp3 C bonds to sp2 C bonds, facilitating the plastic deformation of the coatings and afterward of the substrate. The CrN/DLC displayed superior wear resistance to the DLC-W coating across all temperatures. The DLC-W multilayer coating showed poor wear resistance above 150 °C, being completely removed during the testing. Compared to both coatings, the uncoated 16MnCr5 discs exhibited higher coefficients of friction and wear rates at all temperatures. Predominant wear mechanisms observed in the coated discs were adhesive and abrasive. The study revealed a decrease in the coatings’ structural and mechanical properties with rising temperatures. Hard abrasive WC particles were identified as contributing to increased wear rates in the multilayer DLC-W coatings.
Evaluating the Impact of Filtering Techniques on Deep Learning-Based Brain Tumour Segmentation
Authors: Rosa Sofia , Vasconcelos Verónica , Caridade Pedro J. S. B.
Published: September 2024

Gliomas are a common and aggressive kind of brain tumour that is difficult to diagnose due to their infiltrative development, variable clinical presentation, and complex behaviour, making them an important focus in neuro-oncology. Segmentation of brain tumour images is critical for improving diagnosis, prognosis, and treatment options. Manually segmenting brain tumours is time-consuming and challenging. Automatic segmentation algorithms can significantly improve the accuracy and efficiency of tumour identification, thus improving treatment planning and outcomes. Deep learning-based segmentation tumours have shown significant advances in the last few years. This study evaluates the impact of four denoising filters, namely median, Gaussian, anisotropic diffusion, and bilateral, on tumour detection and segmentation. The U-Net architecture is applied for the segmentation of 3064 contrast-enhanced magnetic resonance images from 233 patients diagnosed with meningiomas, gliomas, and pituitary tumours. The results of this work demonstrate that bilateral filtering yields superior outcomes, proving to be a robust and computationally efficient approach in brain tumour segmentation. This method reduces the processing time by 12 epochs, which in turn contributes to lowering greenhouse gas emissions by optimizing computational resources and minimizing energy consumption.
Geographical Mapping of COVID-19 Testing Rates in St. Louis: Influence of the Socioeconomic Index and Race on Testing in the Early Phase and Peak of the COVID-19 Pandemic
Authors: Governor Samuel B. , Effah William O. , Ntajal Joshua , Nartey Cecilia Naa Ometse , Voytovych Viktoriya , Sam Htu , Ulanja Mark B. , Tutlam Nhial T. , Otchere Prince
Published: September 2024

Purpose: This study aimed to explore how race and the socioeconomic index (SES) of residential zip codes influenced COVID-19 testing rates in St. Louis during the early and peak phases of the pandemic. Method: An ecological study was conducted using COVID-19 testing data from March to November 2020 in St. Louis City, including 16,915 tests from 30 residential zip codes. Geographical mapping identified areas lacking testing, descriptive statistics characterized testing rates by race and SES, and robust linear regression modeled the association between testing rates and race, controlling for SES. Results: The overall testing rate was 303.97 per 10,000 population. Testing rates among Blacks, Whites, American Indians/Alaskan Natives, and Asians were 374.26, 247.77, 360.63, and 242.41 per 10,000, respectively. Higher rates were observed in low- and middle-SES zip codes compared with high-SES areas. Adjusted for SES, testing rates per 10,000 were higher by 125 (1.25%), 448 (4.48%), and 32 (0.32%) among Blacks, American Indians/Alaskan Natives, and Asians compared with Whites. Testing rates decreased by 56 (0.56%) per unit SES improvement. There was statistically significant SES finding and that of the difference between American Indians/Alaskan Natives and Whites. Geographical mapping showed higher rates in the inner core and lower rates in the periphery of St. Louis. Conclusions: Race and SES influence testing rates. Targeted interventions are needed in areas with low testing rates in St. Louis.
Single-Crystal Structure Analysis of Dicarboxamides: Impact of Heteroatoms on Hydrogen Bonding of Carboxamide Groups
Authors: Mohabbat Abdulrahman , Salama Jasmin , Seiffert Philipp , Boldog István , Janiak Christoph
Published: September 2024

This research examines how heteroatoms in a six- or five-membered pyridine, thiophene or furan ring spacer between two carboxamide groups influence the hydrogen bonding for advancements in supramolecular chemistry and drug development. The solvent-free crystal structures of 3,5-pyridinedicarboxamide (PDC), 2,5-thiophenedicarboxamide (TDC) and 2,5-furandicarboxamide (FDC-subl, crystallized by sublimation), and the monohydrate structure of FDC-solv (crystallized from methanol) are described with the hydrogen-bonding analyzed by the Etter graph-set notation. The carbon atoms of the amide groups form an angle of 121° in PDC, 151° in TDC, 137° in FDC-solv and 135° in FDC-subl with the ring centroid. Only in the structure of PDC does the heteroatom act as an H-bond acceptor as part of a C11(6) chain. In TDC and FDC, the heteroatoms do not interact with the amide -NH2 groups.
Surface Electronic Structure of Cr Doped Bi2Se3 Single Crystals
Authors: Yilmaz Turgut , Tong Xiao , Dai Zhongwei , Sadowski Jerzy T. , Gu Genda , Shimada Kenya , Hwang Sooyeon , Kisslinger Kim , Vescovo Elio , Sinkovic Boris
Published: September 2024

Here, by using angle-resolved photoemission spectroscopy, we showed that Bi2−xCrxSe3 single crystals have a distinctly well-defined band structure with a large bulk band gap and undistorted topological surface states. These spectral features are unlike their thin film forms in which a large nonmagnetic gap with a distorted band structure was reported. We further provide laser-based high resolution photoemission data which reveal a Dirac point gap even in the pristine sample. The gap becomes more pronounced with Cr doping into the bulk of Bi2Se3. These observations show that the Dirac point can be modified by the magnetic impurities as well as the light source.
General Trends in the Compression of Glasses and Liquids
Authors: Tschauner Oliver
Published: September 2024

The present work relates the isothermal volumes of silicate glasses and melts to the combined ionic volumes of their chemical constituents. The relation is an extension of a relation that has already been established for crystalline oxides, silicates, alumosilicates, and other materials that have O2− as a constituent anion. The relation provides constraints on bond coordination, indicates pressure-induced changes in coordination in melts and glasses and interatomic distances, and quantifies the extent of transitory regions in pressure-induced coordination changes.