bluuebunny/crossref_metadata_2025 huggingface dataset:
Diversity analyses in two ornamental and large-genome Ranunculaceae species based on a low-cost Klenow NGS-based protocol
Authors: Martina Matteo , Acquadro Alberto , Portis Ezio , Barchi Lorenzo , Lanteri Sergio
Published: June 2023

Persian buttercup (Ranunculus asiaticus L.) and poppy anemone (Anemone coronaria L.) are ornamental, outcrossing, perennial species belonging to the Ranunculaceae family, characterized by large and highly repetitive genomes. We applied K-seq protocol in both species to generate high-throughput sequencing data and produce a large number of genetic polymorphisms. The technique entails the application of Klenow polymerase-based PCR using short primers designed by analyzing k-mer sets in the genome sequence. To date the genome sequence of both species has not been released, thus we designed primer sets based on the reference the genome sequence of the related species Aquilegia oxysepala var. kansuensis (Brühl). A whole of 11,542 SNPs were selected for assessing genetic diversity of eighteen commercial varieties of R. asiaticus, while 1,752 SNPs for assessing genetic diversity in six cultivars of A. coronaria. UPGMA dendrograms were constructed and in R. asiaticus integrated in with PCA analysis. This study reports the first molecular fingerprinting within Persian buttercup, while the results obtained in poppy anemone were compared with a previously published SSR-based fingerprinting, proving K-seq to be an efficient protocol for the genotyping of complex genetic backgrounds.
“Is this blueberry ripe?”: a blueberry ripeness detection algorithm for use on picking robots
Authors: Liu Yan , Zheng Hongtao , Zhang Yonghua , Zhang Qiujie , Chen Hongli , Xu Xueyong , Wang Gaoyang
Published: June 2023

Blueberries are grown worldwide because of their high nutritional value; however, manual picking is difficult, and expert pickers are scarce. To meet the real needs of the market, picking robots that can identify the ripeness of blueberries are increasingly being used to replace manual operators. However, they struggle to accurately identify the ripeness of blueberries because of the heavy shading between the fruits and the small size of the fruit. This makes it difficult to obtain sufficient information on characteristics; and the disturbances caused by environmental changes remain unsolved. Additionally, the picking robot has limited computational power for running complex algorithms. To address these issues, we propose a new YOLO-based algorithm to detect the ripeness of blueberry fruits. The algorithm improves the structure of YOLOv5x. We replaced the fully connected layer with a one-dimensional convolution and also replaced the high-latitude convolution with a null convolution based on the structure of CBAM, and finally obtained a lightweight CBAM structure with efficient attention-guiding capability (Little-CBAM), which we embedded into MobileNetv3 while replacing the original backbone structure with the improved MobileNetv3. We expanded the original three-layer neck path by one to create a larger-scale detection layer leading from the backbone network. We added a multi-scale fusion module to the channel attention mechanism to build a multi-method feature extractor (MSSENet) and then embedded the designed channel attention module into the head network, which can significantly enhance the feature representation capability of the small target detection network and the anti-interference capability of the algorithm. Considering that these improvements will significantly extend the training time of the algorithm, we used EIOU_Loss instead of CIOU_Loss, whereas the k-means++ algorithm was used to cluster the detection frames such that the generated predefined anchor frames are better adapted to the scale of the blueberries. The algorithm in this study achieved a final mAP of 78.3% on the PC terminal, which was 9% higher than that of YOLOv5x, and the FPS was 2.1 times higher than that of YOLOv5x. By translating the algorithm into a picking robot, the algorithm in this study ran at 47 FPS and achieved real-time detection well beyond that achieved manually.
A 10-year prospectus for mathematical epidemiology
Authors: Orr Mark , Mortveit Henning S. , Lebiere Christian , Pirolli Pete
Published: June 2023

There is little significant work at the intersection of mathematical and computational epidemiology and detailed psychological processes, representations, and mechanisms. This is true despite general agreement in the scientific community and the general public that human behavior in its seemingly infinite variation and heterogeneity, susceptibility to bias, context, and habit is an integral if not fundamental component of what drives the dynamics of infectious disease. The COVID-19 pandemic serves as a close and poignant reminder. We offer a 10-year prospectus of kinds that centers around an unprecedented scientific approach: the integration of detailed psychological models into rigorous mathematical and computational epidemiological frameworks in a way that pushes the boundaries of both psychological science and population models of behavior.
The four-factor personality model and its qualitative correlates among opioid agonist therapy clients
Authors: Mahu Ioan T. , Conrod Patricia J. , Barrett Sean P. , Sako Aïssata , Swansburg Jennifer , Stewart Sherry H.
Published: June 2023

Background

The Four Factor Personality Vulnerability model identifies four specific personality traits (e.g., sensation seeking [SS], impulsivity [IMP], anxiety sensitivity [AS], and hopelessness [HOP]) as implicated in substance use behaviors, motives for substance use, and co-occurring psychiatric conditions. Although the relationship between these traits and polysubstance use in opioid agonist therapy (OAT) clients has been investigated quantitatively, no study has examined the qualitative expression of each trait using clients’ voice.

Method

Nineteen Methadone Maintenance Therapy (MMT) clients (68.4% male, 84.2% white, mean age[SD] = 42.71 [10.18]) scoring high on one of the four personality traits measured by the Substance Use Risk Profile Scale [SURPS] completed a semi-structured qualitative interview designed to explore their lived experience of their respective trait. Thematic analysis was used to derive themes, which were further quantified using content analysis.

Results

Themes emerging from interviews reflected (1) internalizing and externalizing symptoms, (2) adversity experiences, and (3) polysubstance use. Internalizing symptoms subthemes included symptoms of anxiety, fear, stress, depression, and avoidance coping. Externalizing subthemes included anger, disinhibited cognitions, and anti-social and risk-taking behaviors. Adverse experiences subthemes included poor health, poverty, homelessness, unemployment, trauma, and conflict. Finally, polysubstance use subthemes include substance types, methods of use, and motives. Differences emerged between personality profiles in the relative endorsement of various subthemes, including those pertaining to polysubstance use, that were largely as theoretically expected.

Conclusion

Personality is associated with unique cognitive, affective, and behavioral lived experiences, suggesting that personality may be a novel intervention target in adjunctive psychosocial treatment for those undergoing OAT.
Electroconvulsive therapy triggers a reversible decrease in brain N-acetylaspartate
Authors: Erchinger Vera J. , Craven Alexander R. , Ersland Lars , Oedegaard Ketil J. , Bartz-Johannessen Christoffer A. , Hammar Åsa , Haavik Jan , Riemer Frank , Kessler Ute , Oltedal Leif
Published: June 2023

Introduction

Based on previous research on electroconvulsive therapy (ECT) we have proposed a model where disruption, potentiation, and rewiring of brain networks occur in sequence and serve as the underlying therapeutic mechanism of ECT. This model implies that a temporary disturbance of neuronal networks (disruption) is followed by a trophic effect (potentiation), which enables the rewiring of neuronal circuits to a more euthymic functioning brain. We hypothesized that disruption of neuronal networks could trigger biochemical alterations leading to a temporary decrease in N-acetylaspartate (tNAA, considered a marker of neuronal integrity), while choline (a membrane component), myo-Inositol (mI, astroglia marker), and glutamate/glutamine (Glx, excitatory neurotransmitter) were postulated to increase. Previous magnetic resonance spectroscopy studies, reporting diverse findings, have used two different referencing methods - creatine ratios and tissue corrected values referenced to water – for the quantification of brain metabolites. Changes in creatine during ECT have also been reported, which may confound estimates adopting this as an internal reference.

Methods

Using MR spectroscopy, we investigated 31 moderately to severely depressed patients and 19 healthy controls before, during, and after ECT or at similar time points (for controls). We tested whether biochemical alterations in tNAA, choline, mI, and Glx lend support to the disrupt, potentiate, and rewire hypothesis. We used both creatine ratios and water-scaled values for the quantification of brain metabolites to validate the results across referencing methods.

Results

Levels of tNAA in the anterior cingulate cortex decreased after an ECT treatment series (average 10.6 sessions) by 6% (p = 0.007, creatine ratio) and 3% (p = 0.02, water referenced) but returned to baseline 6 months after ECT. Compared to after treatment series tNAA levels at 6-month follow-up had increased in both creatine ratio (+6%, p < 0.001) and water referenced data (+7%, p < 0.001). Findings for other brain metabolites varied and could not be validated across referencing methods.

Discussion

Our findings suggest that prior research must be interpreted with care, as several referencing and processing methods have been used in the past. Yet, the results for tNAA were robust across quantification methods and concur with relevant parts of the disrupt, potentiate, and rewire model.
Critical national response in coping with Omicron variant in China, Israel, South Africa, and the United States
Authors: Jiao Jun , Shi Leiyu , Chen Haiqian , Wang Xiaohan , Yang Manfei , Yang Junyan , Liu Meiheng , Sun Gang
Published: June 2023

Objective

The aim of this study was to analyze the effectiveness of prevention and control strategies and put forward further measures according to the epidemiological characteristics of Omicron. It summarized the national response during the Omicron epidemic in four countries: China, Israel, South Africa, and the United States.

Methods

This study summarized prevention and control measures in China, Israel, South Africa, and the United States in their response to the Omicron epidemic, and it also evaluated the effectiveness of these measures.

Results

After the Omicron variant emerged, China and Israel adopted containment strategies, using the “dynamic zero” policy and country closure measures. Meanwhile, South Africa and the United States adopted mitigation strategies, which virtually abandoned social interventions and only focused on medical measures and vaccines. From the first day of reported Omicron cases to 28 February 2022, the four countries reported the following cases: China reported 9,670 new confirmed cases and no deaths, with total deaths per million of 3.21; Israel reported 2,293,415 new confirmed cases and 2,016 deaths, with total deaths per million of 1,097.21; South Africa reported 731,384 new confirmed cases and 9,509 deaths, with total deaths per million reaching 1,655.708; the United States reported 3,042,743 new confirmed cases and 1,688,851 deaths, with total deaths per million reaching 2,855.052, which was much higher than the other countries.

Conclusion

Based on this study, it seems that China and Israel adopted containment strategies, while South Africa and the United States adopted mitigation strategies. A rapid response is a powerful weapon against the Omicron epidemic. Vaccines alone will not get any country out of this crisis, and non-pharmacological measures should be used in addition to them. According to the SPO model, future work should consider the strengthening of emergency management capacity, adhering to public health measures, promoting vaccination, and strengthening patient care and close contact management, which are effective measures in coping with Omicron.
Effectiveness of the Program to Encourage Active, Rewarding Lives (PEARLS) to reduce depression: a multi-state evaluation
Authors: Smith Matthew Lee , Steinman Lesley E. , Montoya Carol N. , Thompson Meghan , Zhong Lixian , Merianos Ashley L.
Published: June 2023

Introduction

An estimated 15% of community-dwelling older adults have depressive symptoms in the U.S. The Program to Encourage Active, Rewarding Lives (PEARLS) is an evidence-based program for managing late-life depression. PEARLS is a home/community-based collaborative care model delivered by community-based organizations to improve access to quality depression care. Trained staff actively screen for depression to improve recognition, teach problem-solving and activity planning skills for self-management, and connect participants to other supports and services as needed.

Methods

This study examined 2015–2021 data from 1,155 PEARLS participants across four states to assess PEARLS effectiveness to reduce depressive symptoms. The clinical outcomes were measured by the self-reported PHQ-9 instrument to assess changes in depressive symptoms scored as depression-related severity, clinical remission, and clinical response. A generalized estimating equation (GEE) model was fitted to examine changes in composite PHQ-9 scores from baseline to the final session. The model adjusted for participants’ age, gender, race/ethnicity, education level, income level, marital status, number of chronic conditions, and number of PEARLS sessions attended. Cox proportional hazards regression models were conducted to estimate the hazard ratio for improvement of depressive symptoms (i.e., remission or response), while adjusting for the covariates.

Results

PHQ-9 scale scores significantly improved from baseline to their final sessions (mean difference = −5.67, SEM = 0.16, p < 0.001). About 35% of participants achieved remission with PHQ-9 score < 5. Compared to participants with mild depression, patients with moderate depression (HR = 0.43, 95%CI = 0.35–0.55), moderately severe depression (HR = 0.28, 95%CI = 0.21–0.38), and severe depression (HR = 0.22 95%CI = 0.14–0.34) were less likely to experience clinical remission with PHQ-9 score < 5, while adjusting for the covariates. About 73% achieved remission based on no longer having one or both cardinal symptoms. Compared to participants with mild depression, patients with moderate depression (HR = 0.66, 95%CI = 0.56–0.78), moderately severe depression (HR = 0.46, 95%CI = 0.38–0.56), and severe depression (HR = 0.38, 95%CI = 0.29–0.51) were less likely to experience clinical remission, while adjusting for the covariates. Nearly 49% of participants had a clinical response or a ≥ 50% decrease in PHQ-9 scores over time. There were no differences between the severity of depression groups based on the time to clinical response.

Discussion

Findings confirm that PEARLS is an effective program to improve depressive symptoms among older adults in diverse real-world community settings and can be a more accessible option for depressive older adults who are traditionally underserved by clinical care.
Discomforting surplus: gender, sexualization, and omissions in ethnographic fieldwork
Authors: Su Phung N. , Su Phi Hong
Published: June 2023

As sisters and sociologists, we shared an unnerving experience of sexual harassment in one of our preliminary field sites. Our research pursuits split thereafter, with one of us leaning into questions of gender and sexuality and the other steering clear. Despite our diverging interests, we both encountered discomforting moments that raise questions about what data we render surplus in our analysis. In this article, we draw on ethnographic and interviewing data from our respective projects to conceptualize “discomforting surplus” as ethnographic data that we omit from our analyses. We offer two types of discomforting surpluses: those that reveal dissonance between our actions and self-conceptions, and those that seem not just uncomfortable, but inconsequential. We mine these discomforting surpluses, calling for introspection about our subject positions and the potential benefits of trying out analytical frames we have ignored. We conclude with practical suggestions for reflecting meaningfully on our relationships to the field and engaging in thought experiments that center discomforting surplus. These contradictions, omissions, and unnerving questions in ethnographic research are important to grapple with as we encounter a push for greater transparency and open science.
Functional semi-finished fish product evaluation: organoleptic and evidence in vivo
Authors: Moldagaliyeva Dinara , Uzakov Yasin , Sarsembayeva Nurzhan , Ibazhanova Assem , Jussipbekova Balzhan , Nurakhova Alma , Artykbayeva Ulbobek , Baimuratova Mairash
Published: June 2023

Introduction

The development of functional products is a new promising trend in the modern food industry. The research aims to confirm the quality indicators, efficacy and safety for living organisms of the developed functional semi-finished fish product—raw smoked sausage. The research was aimed at studying the characteristics of the semi-finished product obtained, including its organoleptic properties. However, it was also important to test the properties of the probiotic component added to the developed product and its effects in vivo on mice to verify the efficacy and safety of the E. coli 64 G strain.

Methods

Organoleptic and histological assessments of the product were performed. A series of experiments were also conducted to assess the product’s safety and functional properties. For this purpose, Enteracol was fed to white outbred laboratory mice with different concentrations of the active ingredient (Escherichia coli 64G strain). Organoleptic properties of the proposed product, such as smell, consumer view, and balanced taste, demonstrated high consumer qualities of the crude smoked sausage with a probiotic component.

Results

The results of the controlled prospective study confirmed that the product is safe and non-toxic to living organisms: mice state alive after consuming a created meal. At the same time, assessing the product’s antagonistic activity revealed its high protective effect (85%–100% survival rate of animals in the experimental groups compared to the control with 100% mortality).

Discussion

The proposed product has a stimulating effect on an organism and demonstrates the antagonistic activity against pathogenic and opportunistic bacteria established in the Enterobacteriaceae family members. The next step will be a long-term study of the product’s stimulating effect to prove its positive impact on the body.
Development of an effective one-step double-antigen sandwich ELISA based on p72 to detect antibodies against African swine fever virus
Authors: Wang Lei , Li Duan , Liu Yanlin , Zhang Leyi , Peng Guoliang , Xu Zheng , Jia Hong , Song Changxu
Published: June 2023

African swine fever (ASF), caused by ASF virus (ASFV), is a highly contagious and lethal disease of domestic pigs leading to tremendous economic losses. As there are no vaccines and drugs available. An effective diagnosis to eliminate ASFV-infected pigs is a crucial strategy to prevent and control ASF. To this end, ASFV capsid protein p72 was expressed using Chinese hamster ovary (CHO) cells and subsequently conjugated with horseradish peroxidase (HRP) to develop a one-step double-antigen sandwich enzyme-linked immunosorbent assay (one-step DAgS-ELISA). The performance of this ELISA for detecting ASFV antibodies was evaluated. Overall, a diagnostic sensitivity of 97.96% and specificity of 98.96% was achieved when the cutoff value was set to 0.25. No cross-reaction with healthy pig serum and other swine viruses was observed. The coefficients of variation of the intra-assay and inter-assay were both <10%. Importantly, this ELISA could detect antibodies in standard serum with 12,800-fold dilution, and seroconversion started from the 7th day post-inoculation (dpi), showing excellent analytical sensitivity and great utility. Furthermore, compared to the commercial kit, this ELISA had a good agreement and significantly shorter operation time. Collectively, a novel one-step DAgS-ELISA for detecting antibodies against ASFV is developed, which will be reliable and convenient to monitor ASFV infection.
Assessing and improving on-farm biosecurity knowledge and practices among swine producers and veterinarians through online surveys and an educational website in Illinois, United States
Authors: Agrawal Isha , Bromfield Corinne , Varga Csaba
Published: June 2023

There is a growing risk to the health and productivity of the Illinois and United States swine population from foreign and endemic infectious diseases. Effective on-farm biosecurity practices play a pivotal role in preventing these high-consequence pathogens from affecting swine farms. Veterinarians are essential in providing disease prevention advice to swine producers that can help them implement effective biosecurity practices on their farms. Our descriptive study objectives were to assess Illinois swine producers’ and veterinarians’ biosecurity perception, knowledge, and practices to identify knowledge gaps and address these by developing an online educational website. We developed two independent online questionnaires using QualtricsXM software. Swine producer members of the Illinois Pork Producers Association and veterinarians registered with the Illinois State Veterinary Medical Association were contacted via e-mail through their associations and asked to complete an online survey. In total, 13 swine producers across 9 Illinois counties operating 82 farms (8 managed single farms and 5 managed multiple farms) responded to the swine producer survey. Despite some biosecurity awareness among swine producers, the need for a biosecurity-related outreach program was evident. Among the 7 swine veterinarian responders, 5 predominantly treated swine (oversaw an average of 21.6 farms), and 2 were mixed animal practitioners. The swine veterinarian survey showed a disconnect between their biosecurity perception and practices. We developed a biosecurity educational website and used Google Analytics to collect website traffic and user data. The 4 months of data showed good coverage that included the highest proportion of users from the Midwest and North Carolina, the largest swine-production regions in the US, and China and Canada, the leading producers of swine worldwide. The most accessed webpage was the resources page, and the swine diseases page had the highest engagement time. Our study highlights the effectiveness of combining online surveys with an educational website to assess and improve the biosecurity knowledge of swine producers and veterinarians that can be applied to assess and improve the biosecurity knowledge and practices of other livestock farmers.
Effects of sources and levels of dietary supplementary manganese on growing yak’s in vitro rumen fermentation
Authors: Lu Huizhen , Liu Pengpeng , Liu Shujie , Zhao Xinsheng , Bai Binqiang , Cheng Jianbo , Zhang Zijun , Sun Cai , Hao Lizhuang , Xue Yanfeng
Published: June 2023

Introduction

Manganese (Mn) is an essential trace element for livestock, but little is known about the optimal Mn source and level for yak.

Methods

To improve yak’s feeding standards, a 48-h in vitro study was designed to examine the effect of supplementary Mn sources including Mn sulfate (MnSO4), Mn chloride (MnCl2), and Mn methionine (Met-Mn) at five Mn levels, namely 35 mg/kg, 40 mg/kg, 50 mg/kg, 60 mg/kg, and 70 mg/kg dry matter (includes Mn in substrates), on yak’s rumen fermentation.

Results

Results showed that Met-Mn groups showed higher acetate (p < 0.05), propionate, total volatile fatty acids (p < 0.05) levels, ammonia nitrogen concentration (p < 0.05), dry matter digestibility (DMD), and amylase activities (p < 0.05) compared to MnSO4 and MnCl2 groups. DMD (p < 0.05), amylase activities, and trypsin activities (p < 0.05) all increased firstly and then decreased with the increase of Mn level and reached high values at 40–50 mg/kg Mn levels. Cellulase activities showed high values (p < 0.05) at 50–70 mg/kg Mn levels. Microbial protein contents (p < 0.05) and lipase activities of Mn-Met groups were higher than those of MnSO4 and MnCl2 groups at 40–50 mg/kg Mn levels.

Discussion

Therefore, Mn-met was the best Mn source, and 40 to 50 mg/kg was the best Mn level for rumen fermentation of yaks.
RNA in situ hybridization of Escherichia coli in equine endometrial biopsies
Authors: Tukia Elina , Hallman Isa , Penttilä Maarit , Hänninen Satu , Kareskoski Maria
Published: June 2023

Endometritis is one of the major causes of infertility in mares. Escherichia coli and β-haemolytic streptococci are among the bacterial species most frequently isolated from the equine uterus. Some bacteria such as β-hemolytic streptococci, can persist in dormant forms and cause prolonged, latent or recurrent infections. Dormant bacteria may be present despite negative bacterial cultures, and they are resistant to antimicrobial treatment due to their resting metabolic state. The purpose of this study was to study formalin-fixed paraffin-embedded equine endometrial biopsies for the presence and localization of E. coli—bacteria, with a chromogenic RNAscope®-method for detection of E. coli-related 16S ribosomal RNA. Hematoxylin-eosin—stained endometrial biopsies were evaluated to determine the level of inflammation and degeneration. During estrus, samples were taken for endometrial culture and cytology with a double-guarded uterine swab. The samples included eight samples with moderate to severe endometrial inflammation detected in endometrial histopathology, and growth of E. coli in bacterial culture, six samples with moderate to severe endometrial inflammation but negative bacterial culture, and five samples with no endometrial pathology (grade I endometrial biopsy, negative endometrial culture and cytology) serving as controls. Positive and negative control probes were included in the RNA in situ hybridization, and results were confirmed with a fluorescence detection method (fluorescence in situ hybridization). Only unspecific signals of limited size and frequency of occurrence were detected in all samples, with random localization in the endometrium. No samples contained rod-shaped signals corresponding to bacterial findings. In conclusion, there was no evidence of bacterial invasion in the endometrium regardless of the inflammatory status of the biopsy or previous bacterial culture results. According to these findings on a small number of samples, invasion of E. coli is not a common finding in the lamina propria of mares, but these bacteria may also evade detection due to localized foci of infections, or supra-epithelial localization under the cover of biofilm. These bacteria and biofilm covering the epithelium may also be lost during formalin-fixation and processing.
Long-Term Supplementation of Ozonated Sunflower Oil Improves Dyslipidemia and Hepatic Inflammation in Hyperlipidemic Zebrafish: Suppression of Oxidative Stress and Inflammation against Carboxymethyllysine Toxicity
Authors: Cho Kyung-Hyun , Kim Ji-Eun , Bahuguna Ashutosh , Kang Dae-Jin
Published: June 2023

Ozonated sunflower oil (OSO) is a well-known functional oil with antioxidant, antimicrobial, anti-allergic, and skin-moisturizing properties. However, studies on the effects of OSO on high-cholesterol diet (HCD)-induced metabolic disorders have been scarce. In the current study, we aimed to determine the anti-inflammatory effects of OSO on lipid metabolism in adult hypercholesterolemic zebrafish and its embryos. Microinjection of OSO (final 2%, 10 nL) into zebrafish embryos under the presence of carboxymethyllysine (CML, 500 ng) protected acute embryo death up to 61% survival, while sunflower oil (final 2%) showed much less protection at around 42% survival. The microinjection of OSO was more effective than SO to inhibit reactive oxygen species (ROS) production and apoptosis in the CML induced embryo toxicity. Intraperitoneal injection of OSO under the presence of CML protected acute death from CML-induced neurotoxicity with improved hepatic inflammation, less detection of ROS and interleukin (IL)-6, and lowering blood total cholesterol (TC) and triglyceride (TG), while the SO-injected group did not protect the CML-toxicity. Long-term supplementation of OSO (final 20%, wt/wt) with HCD for 6 months resulted in higher survivability than the HCD alone group or HCD + SO group (final 20%, wt/wt) with significant lowering of plasma TC and TG levels. The HCD + OSO group showed the least hepatic inflammation, fatty liver change, ROS, and IL-6 production. In conclusion, short-term treatment of OSO by injection exhibited potent anti-inflammatory activity against acute neurotoxicity of CML in zebrafish and their embryo. Long-term supplementation of OSO in the diet also revealed the highest survivability and blood lipid-lowering effect through potent antioxidant and anti-inflammatory activity.
The Impact of Nigella sativa Essential Oil on T Cells in Women with Hashimoto’s Thyroiditis
Authors: Ciesielska-Figlon Klaudia , Wojciechowicz Karolina , Daca Agnieszka , Kokotkiewicz Adam , Łuczkiewicz Maria , Witkowski Jacek Maciej , Lisowska Katarzyna Aleksandra
Published: June 2023

Background: Hashimoto’s thyroiditis (HT) is an autoimmune disease mediated by T cells. It is characterized by the presence of thyroid autoantibodies in the serum, such as anti-thyroid peroxidase antibodies (TPO-Ab) and anti-thyroglobulin antibodies (TG-Ab). The essential oil extracted from Nigella sativa seeds is rich in bioactive substances, such as thymoquinone and cymene. Methods: Therefore, we examined the effect of essential oil from Nigella sativa (NSEO) on T cells from HT patients, especially their proliferation capacity, ability to produce cytokines, and susceptibility to apoptosis. Results: The lowest ethanol (EtOH) dilution (1:10) of NSEO significantly inhibited the proliferation of CD4+ and CD8+ T cells from HT patients and healthy women by affecting the percentage of dividing cells and the number of cell divisions. In addition, 1:10 and 1:50 NSEO dilutions induced cell death. Different dilutions of NSEO also reduced the concentration of IL-17A and IL-10. In healthy women, the level of IL-4 and IL-2 significantly increased in the presence of 1:10 and 1:50 NSEO dilutions. NSEO did not influence the concentration of IL-6 and IFN-γ. Conclusions: Our study demonstrates that NSEO has a strong immunomodulatory effect on the lymphocytes of HT patients.
Machine Learning-Assisted Tensile Modulus Prediction for Flax Fiber/Shape Memory Epoxy Hygromorph Composites
Authors: Sadat Tarik
Published: June 2023

Flax fiber/shape memory epoxy hygromorph composites are a promising area of research in the field of biocomposites. This paper focuses on the tensile modulus of these composites and investigates how it is affected by factors such as fiber orientation (0° and 90°), temperature (20 °C, 40 °C, 60 °C, 80 °C, and 100 °C), and humidity (50% and fully immersed) conditions. Machine learning algorithms were utilized to predict the tensile modulus based on non-linearly dependent initial variables. Both decision tree (DT) and random forest (RF) algorithms were employed to analyze the data, and the results showed high coefficient of determination R2 values of 0.94 and 0.95, respectively. These findings demonstrate the effectiveness of machine learning in analyzing large datasets of mechanical properties in biocomposites. Moreover, the study revealed that the orientation of the flax fibers had the greatest impact on the tensile modulus value (with feature importance of 0.598 and 0.605 for the DT and RF models, respectively), indicating that it is a crucial factor to consider when designing these materials.
Wearable Electromyography Classification of Epileptic Seizures: A Feasibility Study
Authors: Djemal Achraf , Bouchaala Dhouha , Fakhfakh Ahmed , Kanoun Olfa
Published: June 2023

Accurate diagnosis and classification of epileptic seizures can greatly support patient treatments. As many epileptic seizures are convulsive and have a motor component, the analysis of muscle activity can provide valuable information for seizure classification. Therefore, this paper present a feasibility study conducted on healthy volunteers, focusing on tracking epileptic seizures movements using surface electromyography signals (sEMG) measured on human limb muscles. For the experimental studies, first, compact wireless sensor nodes were developed for real-time measurement of sEMG on the gastrocnemius, flexor carpi ulnaris, biceps brachii, and quadriceps muscles on the right side and the left side. For the classification of the seizure, a machine learning model has been elaborated. The 16 common sEMG time-domain features were first extracted and examined with respect to discrimination and redundancy. This allowed the features to be classified into irrelevant features, important features, and redundant features. Redundant features were examined with the Big-O notation method and with the average execution time method to select the feature that leads to lower complexity and reduced processing time. The finally selected six features were explored using different machine learning classifiers to compare the resulting classification accuracy. The results show that the artificial neural network (ANN) model with the six features: IEMG, WAMP, MYOP, SE, SKEW, and WL, had the highest classification accuracy (99.95%). A further study confirms that all the chosen eight sensors are necessary to reach this high classification accuracy.
A Review on the Impact of the SARS-CoV-2 Omicron Subvariant on Elderly Patients with Diverse Co-Morbidities
Authors: Khadela Avinash , Soni Shruti , Megha Kaivalya , Bhagat Shivam , Chavda Vivek
Published: June 2023

The SARS-CoV-2 virus has caused a catastrophic impact on the world for the past 3 years. The virus has now returned with the emergence of the Omicron (B.1.1.529) variant. Within two months of its first emergence in South Africa, Omicron became the most dominating SARS-CoV-2 variant around the world, being the cause of the majority of new infections at present. Omicron has presented with the greatest transmission rate of all the previous variants despite the presence of mass vaccinations and acquired immunity. Several monoclonal antibodies and mRNA vaccines have failed to produce desired effects owing to a large number of mutations present in the Omicron variant. The introduction of the booster dose of the present mRNA vaccines has proven to be a great addition to the therapeutic armamentarium against the Omicron variant. Immunocompromised patients including the elderly, cancer patients, organ transplant recipients, and those with multiple comorbidities have been at a greater risk of developing severe diseases since the pre-Omicron era. The emergence of Omicron again raised a threat against this population. The protection from severe disease and mortality rates through the utilization of multiple immunizations and monoclonal antibodies has been controversial in this subgroup of patients. Thus, designing large-scale studies to evaluate the effectiveness of monoclonal antibodies and vaccines in these patients can provide evidence-based recommendations to improve survival in this population. This article attempts to discuss the different subvariants of Omicron, differences in the mutational aspects along with the particular focus on the consequences of the Omicron infection in the elderly population with diverse comorbidities.
Diagnosis and Management of Mucopolysaccharidosis Type II (Hunter Syndrome) in Poland
Authors: Żuber Zbigniew , Kieć-Wilk Beata , Kałużny Łukasz , Wierzba Jolanta , Tylki-Szymańska Anna
Published: June 2023

Mucopolysaccharidosis type II (MPS II; also known as Hunter syndrome) is a rare, inherited lysosomal storage disease. The disease is caused by deficiency of the lysosomal enzyme iduronate-2-sulphatase (I2S) due to mutations in the IDS gene, which leads to accumulation of glycosaminoglycans (GAGs). Deficiency of I2S enzyme activity in patients with MPS II leads to progressive lysosomal storage of GAGs in the liver, spleen, heart, bones, joints, and respiratory tract. This process disturbs cellular functioning and leads to multisystemic disease manifestations. Symptoms and their time of onset differ among patients. Diagnosis of MPS II involves assessment of clinical features, biochemical parameters, and molecular characteristics. Life-long enzyme replacement therapy with idursulfase (recombinant human I2S) is the current standard of care. However, an interdisciplinary team of specialists is required to monitor and assess the patient’s condition to ensure optimal care. An increasing number of patients with this rare disease reach adulthood and old age. The transition from pediatric care to the adult healthcare system should be planned and carried out according to guidelines to ensure maximum benefit for the patient.
Non-Classical HLA Class 1b and Hepatocellular Carcinoma
Authors: De Re Valli , Tornesello Maria Lina , Racanelli Vito , Prete Marcella , Steffan Agostino
Published: June 2023

A number of studies are underway to gain a better understanding of the role of immunity in the pathogenesis of hepatocellular carcinoma and to identify subgroups of individuals who may benefit the most from systemic therapy according to the etiology of their tumor. Human leukocyte antigens play a key role in antigen presentation to T cells. This is fundamental to the host’s defense against pathogens and tumor cells. In addition, HLA-specific interactions with innate lymphoid cell receptors, such those present on natural killer cells and innate lymphoid cell type 2, have been shown to be important activators of immune function in the context of several liver diseases. More recent studies have highlighted the key role of members of the non-classical HLA-Ib and the transcript adjacent to the HLA-F locus, FAT10, in hepatocarcinoma. The present review analyzes the major contribution of these molecules to hepatic viral infection and hepatocellular prognosis. Particular attention has been paid to the association of natural killer and Vδ2 T-cell activation, mediated by specific HLA class Ib molecules, with risk assessment and novel treatment strategies to improve immunotherapy in HCC.
Research Progress of Dendritic Cell Surface Receptors and Targeting
Authors: Tong Chunyu , Liang Yimin , Han Xianle , Zhang Zhelin , Zheng Xiaohui , Wang Sen , Song Bocui
Published: June 2023

Dendritic cells are the only antigen-presenting cells capable of activating naive T cells in humans and mammals and are the most effective antigen-presenting cells. With deepening research, it has been found that dendritic cells have many subsets, and the surface receptors of each subset are different. Specific receptors targeting different subsets of DCs will cause different immune responses. At present, DC-targeted research plays an important role in the treatment and prevention of dozens of related diseases in the clinic. This article focuses on the current status of DC surface receptors and targeted applications.
Optimization Design for Steel Trusses Based on a Genetic Algorithm
Authors: Li Pengcheng , Zhao Xuxiang , Ding Dangsheng , Li Xiwei , Zhao Yanjun , Ke Lu , Zhang Xiaoyue , Jian Bin
Published: June 2023

Steel trusses are widely utilized in engineering structures, and their optimization is essential for enhancing structural performance and reducing material consumption. Existing optimization methods for steel trusses predominantly rely on the trial-and-error method, which is not only inefficient but also inaccurate. Therefore, this study focused on the optimization of steel trusses using an efficient and accurate optimization methodology. Based on a genetic algorithm and the finite element method, both mono- and multi-parameter optimization designs for steel trusses were executed, an applicable optimization design method for steel trusses was established, and corresponding optimization design programs were developed. The analysis demonstrates that the proposed optimization method effectively optimizes truss height and member cross-section, leading to a significant reduction in material consumption. Compared to the traditional trial-and-error method, the proposed optimization method exhibits adequate calculation accuracy and superior optimization efficiency, thereby providing a robust theoretical foundation for the engineering design of steel trusses.
Rainwater Harvesting as Sustainable Solution to Cope with Drinking Water Scarcity and Urban Flooding: A Case Study of Public Institutions in Lahore, Pakistan
Authors: Waseem Muhammad , Mutahir Ullah Ghazi Syed , Ahmed Nameer , Ayaan Muhammad , Kebede Leta Megersa
Published: June 2023

Pakistan is currently facing physical and economic water scarcity issues, which have been further complicated by the rapid increase in its population and climate change. In affected areas, many methods are being used to tackle this problem, among which rainwater harvesting (RWH) provides the best alternative source of domestic water supply. In rainwater harvesting, a mechanism is designed to effectively collect surface runoff during rainfall events from residential rooftops. It has also been found that rainwater has great potential as a source of water supply in residential areas of major cities, such as Lahore, which is the focus of our study. This research paper examines rainwater harvesting as a sustainable solution to address the challenges of drinking water scarcity and urban flooding. The study discusses the benefits of rainwater harvesting, including reducing reliance on municipal water sources, improving water quality, and mitigating the impact of urban flooding. Additionally, the paper explores the use of filtered water points in conjunction with rainwater harvesting systems to provide clean drinking water to communities. The research draws on case studies from various regions to illustrate the effectiveness of rainwater harvesting as a sustainable solution to water scarcity and urban flooding. Ultimately, the study concludes that rainwater harvesting, when coupled with filtered water points, can offer an effective and sustainable solution to address drinking water scarcity and urban flooding.
Research on IR and Heat Transfer Characteristics of Molybdenum-Sputtered Polyamide Materials
Authors: Han Hye-Ree
Published: June 2023

Demand for the development of the convergence industry, research studies on heat transfer, thermal characteristics, semiconductors, motors, and batteries using special materials have come to the fore. Meanwhile, molybdenum (Mo) exhibits relatively small inorganic qualities, and the thermal conductivity rate is applied to various fields. In this study, in-depth characteristics were considered regarding the concentration of thermal characteristics and IR characteristics. In particular, this study conducted a thicker molybdenum layer sputtering work than previous studies and examined it in detail at high temperatures by pore size. This study calculated each phase temperature of the molybdenum-sputtered specimens in the steady state according to the heat transfer theory. When the molybdenum-sputtered fabric’s metal layer pointed to the outside air, the heat transfer rate (Q) was high at 5748.3 W. In contrast, if the molybdenum-sputtered film’s metal layer pointed toward the heat source, the heat transfer rate (Q) was low at 187.1 W. As a result of measuring the IR transmittance, the infrared transmittance of the molybdenum-sputtering-treated sample was significantly reduced compared to the untreated sample. It is believed that the molybdenum-sputtering polyamide samples produced in this study can be applied to multifunctional military wear, biosignal detection sensors, semiconductor products, batteries, etc., by utilizing excellent electrical properties, stealth functions, and IR-blocking properties.
Assessing the Impact of Insect Decline in Islands: Exploring the Diversity and Community Patterns of Indigenous and Non-Indigenous Arthropods in the Azores Native Forest over 10 Years
Authors: Lhoumeau Sébastien , Borges Paulo A. V.
Published: June 2023

The ongoing decline of insect populations highlight the need for long-term ecological monitoring. As part of the “SLAM—Long Term Ecological Study of the Impacts of Climate Change on the Natural Forests of Azores” project, we investigated changes in arthropod diversity and community structure over a ten-year period (2012–2022) in the native forest of the island of Terceira (Azores). Focused on two arthropod assemblages (indigenous and non-indigenous species) monitored with SLAM traps, we asked if there was a distinguishable pattern in the diversity and structure of the studied arthropod subsets in a pristine island native forest. Species richness remained relatively constant. Endemic arthropods dominated and remained stable over time, indicating the forest’s ecological stability. In contrast, the assemblage of native non-endemic arthropods underwent changes, including increased hyperdominance and decreased biomass. The introduced arthropod assemblage showed more erratic dynamics driven by species turnover. Results suggested that temporal variation in each subset may be due to different ecological processes and that niche filtering may limit the establishment and spread of introduced arthropods. This research contributes to our understanding of the temporal dynamics of arthropods in native island forests and highlights the need for ongoing conservation efforts to protect these fragile ecosystems.
Diversity of True Bugs (Hemiptera: Heteroptera) on Common Ragweed (Ambrosia artemisiifolia) in Southern Slovakia
Authors: Tóth Peter , Tóthová Monika , Krchňavá Veronika , Ščevková Jana
Published: June 2023

The common ragweed (Ambrosia artemisiifolia Linnaeus 1800) is an exceptionally invasive species. The information on true bugs occurring on ragweed plants is limited in the invasion region. The objective of this study was to determine the species composition of Heteroptera associated with A. artemisiifolia, to assess their vectoring potential based on a literature review, and to compare species similarity in the surveyed fields. Field surveys were conducted in 2020–2021 at 10 sites in southern Slovakia. Sweeping and visual observations were conducted in field margins, weedy agricultural fields, and mowed meadows infested with A. artemisiifolia. In the study, food specialization, the abundance of individual species, and their assignment to families were precisely determined. The Jaccard similarity index was used to evaluate similarities in species composition among the sites studied. A total of 2496 true bugs were recorded, representing 47 species of Heteroptera from 12 families. The most common phytophagous species were Nysius ericae ericae (Schilling, 1829) (Pentatomomorpha, Lygaeidae), Adelphocoris lineolatus (Goeze, 1778), Lygus rugulipennis (Poppius, 1911), Lygus pratensis (Linnaeus, 1758) (Cimicomorpha, Miridae), and a zoophagous species Nabis (Dolichonabis) limbatus (Dahlbom, 1851) (Cimicomorpha, Nabidae). The species similarities in pair-wise combined localities were low, with a dominance of highly migratory and polyphagous species able to traverse the field from the adjacent landscape. A. artemisiifolia is a known host for plant viruses and phytoplasmas, and several Heteroptera species are carriers of these plant pathogens. Halyomorpha halys was the only detected vector of phytoplasmas, and its abundance on A. artemisiifolia was extremely low.