bluuebunny/crossref_metadata_2025 huggingface dataset:
Patient and Medical Oncologists’ Perspectives on Prescribed Lifestyle Intervention—Experiences of Women with Breast Cancer and Providers
Authors: Balneaves Lynda G. , Truant Tracy L. O. , Van Patten Cheri , Kirkham Amy A. , Waters Erin , Campbell Kristin L.
Published: August 2020

This study explored the perspectives and experiences of breast cancer patients and medical oncologists with regards to participation in a lifestyle intervention at a tertiary cancer treatment center. A thematic approach was used to understand the context within which a lifestyle intervention was recommended and experienced, to inform future lifestyle programming and promote uptake. Twelve women with breast cancer receiving adjuvant chemotherapy and eight medical oncologists completed interviews. Findings suggest receiving a prescription for a lifestyle intervention from a trusted health professional was influential to women with breast cancer. The intervention offered physical, psychological, emotional, social, and informational benefits to the women and oncologists perceived both physiological and relational benefit to prescribing the intervention. Challenges focused on program access and tailored interventions. Lifestyle prescriptions are perceived by women with breast cancer to have numerous benefits and may promote lifestyle interventions and build rapport between oncologists and women. Oncology healthcare professionals play a pivotal role in motivating women’s participation in lifestyle interventions during breast cancer treatment. Maintenance programs that transition patients into community settings and provide on-going information and follow-up are needed.
Anti-IL-6 Receptor Tocilizumab in Refractory Graves’ Orbitopathy: National Multicenter Observational Study of 48 Patients
Authors: Sánchez-Bilbao Lara , Martínez-López David , Revenga Marcelino , López-Vázquez Ángel , Valls-Pascual Elia , Atienza-Mateo Belén , Valls-Espinosa Beatriz , Maiz-Alonso Olga , Blanco Ana , Torre-Salaberri Ignacio , Rodríguez-Méndez Verónica , García-Aparicio Ángel , Veroz-González Raúl , Jovaní Vega , Peiteado Diana , Sánchez-Orgaz Margarita , Tomero Eva , Toyos-Sáenz de Miera Francisco J. , Pinillos Valvanera , Aurrecoechea Elena , Mora Ángel , Conesa Arantxa , Fernández-Prada Manuel , Troyano Juan A. , Calvo-Río Vanesa , Demetrio-Pablo Rosalía , González-Mazón Íñigo , Hernández José L. , Castañeda Santos , González-Gay Miguel Á. , Blanco Ricardo
Published: August 2020

Graves’ orbitopathy (GO) is the most common extrathyroidal manifestation of Graves’ disease (GD). Our aim was to assess the efficacy and safety of Tocilizumab (TCZ) in GO refractory to conventional therapy. This was an open-label multicenter study of glucocorticoid-resistant GO treated with TCZ. The main outcomes were the best-corrected visual acuity (BVCA), Clinical Activity Score (CAS) and intraocular pressure (IOP). These outcome variables were assessed at baseline, 1st, 3rd, 6th and 12th month after TCZ therapy onset. The severity of GO was assessed according to the European Group on Graves’ Orbitopathy (EUGOGO). We studied 48 (38 women and 10 men) patients (95 eyes); mean age ± standard deviation 51 ± 11.8 years. Before TCZ and besides oral glucocorticoids, they had received IV methylprednisolone (n = 43), or selenium (n = 11). GO disease was moderate (n =29) or severe (n = 19) and dysthyroid optic neuropathy (DON) (n = 7). TCZ was used in monotherapy (n = 45) or combined (n = 3) at a dose of 8 mg/kg IV every four weeks (n = 43) or 162 mg/s.c. every week (n = 5). TCZ yielded a significant improvement in all of the main outcomes at the 1st month that was maintained at one year. Comparing the baseline with data at 1 year all of the variables improved; BCVA (0.78 ± 0.25 vs. 0.9 ± 0.16; p = 0.0001), CAS (4.64 ± 1.5 vs. 1.05 ± 1.27; p = 0.0001) and intraocular pressure (IOP) (19.05 ± 4.1 vs. 16.73 ± 3.4 mmHg; p = 0.007). After a mean follow-up of 16.1 ± 2.1 months, low disease activity (CAS ≤ 3), was achieved in 88 eyes (92.6%) and TCZ was withdrawn in 29 cases due to low disease activity (n = 25) or inefficacy (n = 4). No serious adverse events were observed. In conclusion, TCZ is a useful and safe therapeutic option in refractory GO treatment.
Portal Thrombosis in Cirrhosis: Role of Thrombophilic Disorders
Authors: Fortea José Ignacio , Carrera Inés García , Puente Ángela , Cuadrado Antonio , Huelin Patricia , Tato Carmen Álvarez , Fernández Paloma Álvarez , Montes María del Rocío Pérez , Céspedes Javier Nuñez , López Ana Batlle , Sanchez Francisco José González , Hoyos Marcos López , Crespo Javier , Fábrega Emilio
Published: August 2020

In patients with liver cirrhosis the contribution of inherited and acquired prothrombotic disorders in the development of non-malignant portal vein thrombosis (PVT) is inconclusive. The purpose of this retrospective study was to examine the prevalence of thrombophilia in this setting at our center from January 2012 to November 2019. Tests included gene mutational analysis for Factor V Leiden, prothrombin G20210A, JAK2 (V617F), Calreticulin (CARL), in addition to activated protein C resistance, antithrombin III, protein C and S levels, and antiphospholipid antibodies. We included 77 patients, six of whom (7.8%) had a thrombophilic disorder: antiphospholipid syndrome in four patients, prothrombin gene mutation in one and factor V Leiden mutation in one. This latter patient had also been diagnosed with polycythemia vera years before PVT development. Complete thrombosis of the main portal vein and re-thrombosis after stopping anticoagulation were more frequent in patients with thrombophilia, but the rates of recanalization under anticoagulant therapy were similar among groups. No other difference was accounted between groups. The low prevalence of acquired and inherited thrombophilia found in patients with cirrhosis and PVT support testing for these disorders on an individual basis and avoiding universal screening to reduce costs and unwarranted testing.
Effects of Physical Rehabilitation on Spatiotemporal Gait Parameters and Ground Reaction Forces of Patients with Intermittent Claudication
Authors: Dziubek Wioletta , Stefańska Małgorzata , Bulińska Katarzyna , Barska Katarzyna , Paszkowski Rafał , Kropielnicka Katarzyna , Jasiński Ryszard , Rachwalik Anna , Woźniewski Marek , Szuba Andrzej
Published: August 2020

Chronic ischemia of the lower extremities often presents as intermittent claudication characterized by lower limb pain which subsides after a short break. This study aimed to provide an assessment of the spatiotemporal parameters of gait and ground reaction forces in patients with PAD participating in three forms of supervised physical training. A total of 80 subjects completed a three-month supervised physical rehabilitation program with three sessions per week. The subjects were assigned to one of three programs: group 1—standard walking training on a treadmill (TT); group 2—Nordic walking (NW) training; group 3—strength and endurance training comprised of NW with isokinetic resistance training (NW + ISO). Gait biomechanics tests (kinematic and kinetic parameters of gait) and a six-minute walk test were carried out before and after three months of physical training. Nordic walking training led to the greatest improvements in the gait pattern of patients with PAD and a significant increase in the absolute claudication distance and total gait distance. Combined training (NW + ISO) by strengthening the muscles of the lower extremities increased the amplitude of the general center of gravity oscillation to the greatest extent. Treadmill training had little effect on the gait pattern. Nordic walking training should be included in the rehabilitation of patients with PAD as a form of gait training, which can be conducted under supervised or unsupervised conditions.
Antagonistic Yeasts: A Promising Alternative to Chemical Fungicides for Controlling Postharvest Decay of Fruit
Authors: Zhang Xiaokang , Li Boqiang , Zhang Zhanquan , Chen Yong , Tian Shiping
Published: August 2020

Fruit plays an important role in human diet. Whereas, fungal pathogens cause huge losses of fruit during storage and transportation, abuse of chemical fungicides leads to serious environmental pollution and endangers human health. Antagonistic yeasts (also known as biocontrol yeasts) are promising substitutes for chemical fungicides in the control of postharvest decay owing to their widespread distribution, antagonistic ability, environmentally friendly nature, and safety for humans. Over the past few decades, the biocontrol mechanisms of antagonistic yeasts have been extensively studied, such as nutrition and space competition, mycoparasitism, and induction of host resistance. Moreover, combination of antagonistic yeasts with other agents or treatments were developed to improve the biocontrol efficacy. Several antagonistic yeasts are used commercially. In this review, the application of antagonistic yeasts for postharvest decay control is summarized, including the antagonistic yeast species and sources, antagonistic mechanisms, commercial applications, and efficacy improvement. Issues requiring further study are also discussed.
The Obligation of Diplomats to Respect the Laws and Regulations of the Hosting State: A Critical Overview of the International Practices
Authors: Ahmad Nehaluddin
Published: August 2020

Under Article 9 of the Vienna Convention on Diplomatic Relations, a receiving state may “at any time and without having to explain its decision” declare any member of a diplomatic staff persona non grata. A person so declared is considered unacceptable and is usually recalled to his or her home nation. If not recalled, the receiving state “may refuse to recognize the person concerned as a member of the mission.” However, despite the codification of the above rules, which is largely based on pre-existing customary international law, the opportunity for diplomatic protection is not free of issues and controversies. In recent times, unfortunately, there has been a growing tendency amongst diplomats to abuse their diplomatic status, in order to commit acts prohibited by law and claim immunity from the legal process. This paper addresses the problem of abuse of immunities and privileges and its adverse implications on the balance between immunities and the duty to respect the local laws and regulations. We analyze several past cases of declaration of persona non grata involving various countries.
LincRNA-p21 Levels Relates to Survival and Post-Operative Radiotherapy Benefit in Rectal Cancer Patients
Authors: Li Yan , Castellano Joan J. , Moreno Isabel , Martínez-Rodenas Francisco , Hernandez Raquel , Canals Jordi , Diaz Tania , Han Bing , Muñoz Carmen , Biete Albert , Monzo Mariano , Navarro Alfons
Published: August 2020

LincRNA-p21 is a long non-coding RNA involved in the p53 pathway and angiogenesis regulation that acts as prognostic marker in several tumors. In the present study, we aimed to analyze the clinical value of lincRNA-p21 in 177 resected stage I–III colorectal cancer (CRC) patients. Tumor and normal paired tissue and plasma samples from tumor-draining mesenteric veins and paired peripheral veins were analyzed. LincRNA-p21 expression was determined by RTqPCR and correlated with disease-free (DFS) and overall survival (OS). LincRNA-p21 was downregulated in tumor versus normal tissue (p = 0.0012). CRC patients with high lincRNA-p21 expression had shorter DFS (p = 0.0372) and shorter OS (p = 0.0465). Of note, the major prognostic impact was observed in the subset of rectal cancer patients where patients with high lincRNA-p21 levels had worse DFS (p = 0.0226) and OS (p = 0.0457). Interestingly, rectal cancer patients with high lincRNA-p21 benefited from post-operative chemoradiotherapy, as indicated by a longer OS in the group of high lincRNA-p21 patients receiving post-operative chemoradiotherapy (p = 0.04). Finally, patients with high lincRNA-p21 levels in mesenteric vein (MV) had shorter OS (p = 0.0329). LincRNA-p21 is a marker of advanced disease and worse outcome in CRC. Moreover, rectal cancer patients with high lincRNA-p21 levels could benefit from post-operative chemoradiotherapy, and plasmatic-lincRNA-p21 is a promising liquid biopsy biomarker.
Enhancement of Thermal and Mechanical Properties of Bismaleimide Using a Graphene Oxide Modified by Epoxy Silane
Authors: Jiang Hao , Ji Yanyan , Gan Jiantuo , Wang Lei
Published: August 2020

A thermosetting resin system, based on bismaleimide (BMI), has been developed via copolymerization of 4,4′-diaminodiphenylsulfone with a newly synthesized graphene oxide modified using epoxy silane (ES-GO). The effect of ES-GO on the thermomechanical and mechanical properties of cured modified resin was studied. To evaluate the efficiency of the modified BMI systems, the composite samples using glass fiber cloth were molded and tested. Thermogravimetric analysis indicates that the cured sample systems displays a high char yield at lower concentrations of ES-GO (≤0.5 wt.%), suggesting an improved thermal stability. Using dynamic mechanical analysis, a marked increase in glass transition temperature (Tg) with increasing ES-GO content was observed. Analysis of mechanical properties reveals a possible effect of ES-GO as a toughener. The results also showed that the addition of 0.3 wt.% ES-GO maximizes the toughness of the modified resin systems, which was further confirmed by the result of analysis of fracture surfaces. At the same time, a molded composite with ES-GO showed improved mechanical properties and retention rate at 150 °C as compared to that made with neat resin.
Design and Characterization of Sheet-Based Gyroid Porous Structures with Bioinspired Functional Gradients
Authors: Jin Yuan , Kong Haoyu , Zhou Xueyong , Li Guangyong , Du Jianke
Published: August 2020

A new type of sheet porous structures with functionally gradients based on triply periodic minimal surfaces (TPMS) is proposed for designing bone scaffolds. The graded structures were generated by constructing branched features with different number of sheets. The design of the structure was formulated mathematically and five types of porous structure with different structural features were used for investigation. The relative density (RD) and surface area to volume (SA/V) ratio of the samples were analyzed using a slice-based approach to confirm their relationships with design parameters. All samples were additively manufactured using selective laser melting (SLM), and their physical morphologies were observed and compared with the designed models. Compression tests were adopted to study the mechanical properties of the proposed structure from the obtained stress–strain curves. The results reveal that the proposed branched-sheet structures could enhance and diversify the physical and mechanical properties, indicating that it is a potential method to tune the biomechanical properties of porous scaffolds for bone tissue engineering (TE).
Dynamics of a SIR Epidemic Model of Childhood Diseases with a Saturated Incidence Rate: Continuous Model and Its Nonstandard Finite Difference Discretization
Authors: Darti Isnani , Suryanto Agus
Published: August 2020

A SIR epidemic model that describes the dynamics of childhood disease with a saturated incidence rate and vaccination program at a constant rate was investigated. For the continuous model we first show its basic properties, namely, the non-negativity and boundedness of solutions. Then we investigate the existence and both local and global stability of the equilibrium points. It was found that the existence and stability properties of equilibrium points fully determined the basic reproduction number. We also propose and analyze a discrete-time analogue of the continuous childhood diseases by applying a nonstandard finite difference method. It is shown that our discrete model preserves the dynamical properties of the corresponding continuous model, such as the positivity solutions, the population conservation law, the existence of equilibrium points and their global stability properties.
Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer
Authors: Cevenini Armando , Orrù Stefania , Imperlini Esther
Published: August 2020

Searching for new cancer-related biomarkers is a key priority for the early detection of solid tumors, such as colorectal cancer (CRC), in clinically relevant biological fluids. The cell line and/or tumor tissue secretome represents a valuable resource for discovering novel protein markers secreted by cancer cells. The advantage of a secretome analysis is the reduction of the large dynamic range characterizing human plasma/serum, and the simultaneous enrichment of low abundance cancer-secreted proteins, thereby overcoming the technical limitations underlying the direct search in blood samples. In this review, we provided a comprehensive overview of recent studies on the CRC secretome for biomarker discovery, focusing both on methodological and technical aspects of secretome proteomic approaches and on biomarker-independent validation in CRC patient samples (blood and tissues). Secretome proteomics are mainly based on LC-MS/MS analyses for which secretome samples are either in-gel or in-solution trypsin-digested. Adequate numbers of biological and technical replicates are required to ensure high reproducibility and robustness of the secretome studies. Moreover, another major challenge is the accuracy of proteomic quantitative analysis performed by label-free or labeling methods. The analysis of differentially expressed proteins in the CRC secretome by using bioinformatic tools allowed the identification of potential biomarkers for early CRC detection. In this scenario, this review may help to follow-up the recent secretome studies in order to select promising circulating biomarkers to be validated in larger screenings, thereby contributing toward a complete translation in clinical practice.
Property Characterization and Mechanism Analysis of Polyoxometalates-Functionalized PVDF Membranes by Electrochemical Impedance Spectroscopy
Authors: Yao Lei , Long Ziyi , Chen Zhe , Cheng Qisong , Liao Yuan , Tian Miao
Published: August 2020

Polyoxometalates (POMs) has proved its advantage in constructing high-performance nanocomposite membranes such as catalytic membranes, adsorptive membranes, and forward osmosis membranes. However, it is challenging or tedious to characterize its distribution and effect on the membrane structures due to the equipment resolution limitation, discrete nano-scaled structures of POMs, and limited doping amount compared to the polymeric membrane matrix. In this paper, POMs-functionalized polyvinylidene fluoride (PVDF) membranes were fabricated by phase inversion combined with the sol-gel method, and electrochemical impedance spectroscopy (EIS) was utilized to analyze the nanocomposite membrane intrinsic properties. Through adjusting the additives in the sol-forming process, a set of membranes with varied intrinsic properties were developed accordingly. The wetting degree of the membranes related to the hydrophilic nature of the membrane surfaces had a crucial influence on the impedance measurements at the early stage. Through EIS analysis, it was demonstrated that the amination of the membrane matrix through (3-aminopropyl)trimethoxysilane (APTMS) treatment and the immobilization of POMs through electrostatic attraction would not generate new pore structures into the membrane and only alter the membrane surface roughness and composition. To my knowledge, it is the first time that EIS was utilized to characterize the hydrophilicity of the membranes and pore structures of the POMs-modified membranes. Our findings indicate that EIS can provide valuable information for probing the structures of other nano-materials-incorporated membranes.
Implications for SARS-CoV-2 Vaccine Design: Fusion of Spike Glycoprotein Transmembrane Domain to Receptor-Binding Domain Induces Trimerization
Authors: Azad Taha , Singaravelu Ragunath , Crupi Mathieu J.F. , Jamieson Taylor , Dave Jaahnavi , Brown Emily E.F. , Rezaei Reza , Taha Zaid , Boulton Stephen , Martin Nikolas T. , Surendran Abera , Poutou Joanna , Ghahremani Mina , Nouri Kazem , Whelan Jack T. , Duong Jessie , Tucker Sarah , Diallo Jean-Simon , Bell John C. , Ilkow Carolina S.
Published: August 2020

The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic presents an urgent need for an effective vaccine. Molecular characterization of SARS-CoV-2 is critical to the development of effective vaccine and therapeutic strategies. In the present study, we show that the fusion of the SARS-CoV-2 spike protein receptor-binding domain to its transmembrane domain is sufficient to mediate trimerization. Our findings may have implications for vaccine development and therapeutic drug design strategies targeting spike trimerization. As global efforts for developing SARS-CoV-2 vaccines are rapidly underway, we believe this observation is an important consideration for identifying crucial epitopes of SARS-CoV-2.
Role of Membrane Technology in Absorption Heat Pumps: A Comprehensive Review
Authors: Ibarra-Bahena Jonathan , Raman Shankar , Galindo-Luna Yuridiana Rocio , Rodríguez-Martínez Antonio , Rivera Wilfrido
Published: August 2020

The role of heat pumps is linked to the actions of human life. Even though the existing technologies perform well in general, they have still some problems, such as cost, installation area, components size, number of components, noise, etc. To address these issues, membrane technologies have been introduced in both heat and cooling devices. The present work proposes and studied the review of the role of membrane technology in the heat pumps. The study focuses on the advancement and replacement of membrane in the place of absorption and compression heat pump components. The detailed analysis and improvements are focused on the absorber, desorber, and heat and mass exchanger. The parameters conditions and operation of membrane technologies are given in detail. In addition to this, the innovation in the heat pumps using the membrane technology is given in detail.
Constitutive Equations for Describing the Warm and Hot Deformation Behavior of 20Cr2Ni4A Alloy Steel
Authors: Wang Haoran , Wang Wei , Zhai Ruixue , Ma Rui , Zhao Jun , Mu Zhenkai
Published: August 2020

Isothermal hot compression tests of 20Cr2Ni4A alloy steel were performed under temperatures of 973–1273 K and strain rates of 0.001–1 s−1. The behavior of the flow stress of 20Cr2Ni4A alloy steel at warm and hot temperatures is complicated because of the influence of the work hardening, the dynamic recovery, and the dynamic recrystallization. Four constitutive equations were used to predict the flow stress of 20Cr2Ni4A alloy steel, including the original strain-compensated Arrhenius-type (osA-type) equation, the new modified strain-compensated Arrhenius-type (msA-type) equation, the original Hensel–Spittel (oHS) equation and the modified Hensel–Spittel (mHS) equation. The msA-type and mHS are developed by revising the deformation temperatures, which can improve prediction accuracy. In addition, we propose a new method of solving the parameters by combining a linear search with multiple linear regression. The new solving method is used to establish the two modified constitutive equations instead of the traditional regression analysis. A comparison of the predicted values based on the four constitutive equations was performed via relative error, average absolute relative error (AARE) and the coefficient of determination (R2). These results show the msA-type and mHS equations are more accurate and efficient in terms of predicting the flow stress of the 20Cr2Ni4A steel at elevated temperature.
Characterization of Sunflower Oil Extracts from the Lichen Usnea barbata
Authors: Basiouni Shereen , Fayed Marwa A. A. , Tarabees Reda , El-Sayed Mohamed , Elkhatam Ahmed , Töllner Klaus-Rainer , Hessel Manfred , Geisberger Thomas , Huber Claudia , Eisenreich Wolfgang , Shehata Awad A.
Published: August 2020

The increasing global emergence of multidrug resistant (MDR) pathogens is categorized as one of the most important health problems. Therefore, the discovery of novel antimicrobials is of the utmost importance. Lichens provide a rich source of natural products including unique polyketides and polyphenols. Many of them display pharmaceutical benefits. The aim of this study was directed towards the characterization of sunflower oil extracts from the fruticose lichen, Usnea barbata. The concentration of the major polyketide, usnic acid, was 1.6 mg/mL extract as determined by NMR analysis of the crude mixture corresponding to 80 mg per g of the dried lichen. The total phenolics and flavonoids were determined by photometric assays as 4.4 mg/mL (gallic acid equivalent) and 0.27 mg/mL (rutin equivalent) corresponding to 220 mg/g and 13.7 mg/g lichen, respectively. Gram-positive (e.g., Enterococcus faecalis) and Gram-negative bacteria, as well as clinical isolates of infected chickens were sensitive against these extracts as determined by agar diffusion tests. Most of these activities increased in the presence of zinc salts. The data suggest the potential usage of U. barbata extracts as natural additives and mild antibiotics in animal husbandry, especially against enterococcosis in poultry.
Plasma Lipid Profiling of Three Types of Drug-Induced Liver Injury in Japanese Patients: A Preliminary Study
Authors: Saito Kosuke , Kagawa Tatehiro , Tsuji Keiji , Kumagai Yuji , Sato Ken , Sakisaka Shotaro , Sakamoto Naoya , Aiso Mitsuhiko , Hirose Shunji , Mori Nami , Tanaka Rieko , Uraoka Toshio , Takata Kazuhide , Ogawa Koji , Mori Kazuhiko , Sato Motonobu , Nishiya Takayoshi , Takamatsu Kazuhiko , Arakawa Noriaki , Izumi Takashi , Ohno Yasuo , Saito Yoshiro , Takikawa Hajime
Published: August 2020

Drug-induced liver injury (DILI) is a major adverse event caused by drug treatment, which can be categorized into three types: hepatocellular, mixed, and cholestatic. Although nearly every class of drugs can cause DILI, an overall understanding of lipid profiles in DILI patients is lacking. We used lipidomics to analyze the plasma lipid profiles of patients to understand their hepatic pathophysiology and identify DILI biomarkers. We identified 463 lipids and compared their levels between the acute and recovery phases of the three types of DILI patients. Mixed and cholestatic types demonstrated specific plasma lipid alterations between the phases, but the hepatocellular type did not. Moreover, as specific indicators of mixed-type DILI, levels of several ceramides increased in the acute phase, while those of arachidonic acid-containing ether-linked phosphoglycerolipids decreased. In contrast, as specific indicators of cholestatic-type DILI, levels of palmitic acid-containing saturated or monounsaturated phosphatidylcholines increased in the acute phase, while those of arachidonic acid- or docosahexaenoic acid-containing ether-linked phosphoglycerolipids and phosphatidylinositols decreased. We also identified lipids with a relatively high capacity to discriminate the acute phase from the recovery phase and healthy subjects. These findings may help with understanding the pathophysiology of different DILI types and identify candidate biomarkers.
Targeted Metabolic Profiling of Urine Highlights a Potential Biomarker Panel for the Diagnosis of Alzheimer’s Disease and Mild Cognitive Impairment: A Pilot Study
Authors: Yilmaz Ali , Ugur Zafer , Bisgin Halil , Akyol Sumeyya , Bahado-Singh Ray , Wilson George , Imam Khaled , Maddens Michael E. , Graham Stewart F.
Published: August 2020

The lack of sensitive and specific biomarkers for the early detection of mild cognitive impairment (MCI) and Alzheimer’s disease (AD) is a major hurdle to improving patient management. A targeted, quantitative metabolomics approach using both 1H NMR and mass spectrometry was employed to investigate the performance of urine metabolites as potential biomarkers for MCI and AD. Correlation-based feature selection (CFS) and least absolute shrinkage and selection operator (LASSO) methods were used to develop biomarker panels tested using support vector machine (SVM) and logistic regression models for diagnosis of each disease state. Metabolic changes were investigated to identify which biochemical pathways were perturbed as a direct result of MCI and AD in urine. Using SVM, we developed a model with 94% sensitivity, 78% specificity, and 78% AUC to distinguish healthy controls from AD sufferers. Using logistic regression, we developed a model with 85% sensitivity, 86% specificity, and an AUC of 82% for AD diagnosis as compared to cognitively healthy controls. Further, we identified 11 urinary metabolites that were significantly altered to include glucose, guanidinoacetate, urocanate, hippuric acid, cytosine, 2- and 3-hydroxyisovalerate, 2-ketoisovalerate, tryptophan, trimethylamine N oxide, and malonate in AD patients, which are also capable of diagnosing MCI, with a sensitivity value of 76%, specificity of 75%, and accuracy of 81% as compared to healthy controls. This pilot study suggests that urine metabolomics may be useful for developing a test capable of diagnosing and distinguishing MCI and AD from cognitively healthy controls.
Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells
Authors: Crowell Lexi , Yakisich Juan , Aufderheide Brian , Adams Tayloria
Published: August 2020

Electrical impedance spectroscopy (EIS) is an electrokinetic method that allows for the characterization of intrinsic dielectric properties of cells. EIS has emerged in the last decade as a promising method for the characterization of cancerous cells, providing information on inductance, capacitance, and impedance of cells. The individual cell behavior can be quantified using its characteristic phase angle, amplitude, and frequency measurements obtained by fitting the input frequency-dependent cellular response to a resistor–capacitor circuit model. These electrical properties will provide important information about unique biomarkers related to the behavior of these cancerous cells, especially monitoring their chemoresistivity and sensitivity to chemotherapeutics. There are currently few methods to assess drug resistant cancer cells, and therefore it is difficult to identify and eliminate drug-resistant cancer cells found in static and metastatic tumors. Establishing techniques for the real-time monitoring of changes in cancer cell phenotypes is, therefore, important for understanding cancer cell dynamics and their plastic properties. EIS can be used to monitor these changes. In this review, we will cover the theory behind EIS, other impedance techniques, and how EIS can be used to monitor cell behavior and phenotype changes within cancerous cells.
A Proof of Principle for the Detection of Viable Brucella spp. in Raw Milk by qPCR Targeting Bacteriophages
Authors: Projahn Michaela , Hammerl Jens A. , Dieckmann Ralf , Dahouk Sascha Al
Published: August 2020

Brucellosis is still a global health issue, and surveillance and control of this zoonotic disease in livestock remains a challenge. Human outbreaks are mainly linked to the consumption of unpasteurized dairy products. The detection of human pathogenic Brucella species in food of animal origin is time-consuming and laborious. Bacteriophages are broadly applied to the typing of Brucella isolates from pure culture. Since phages intracellularly replicate to very high numbers, they can also be used as specific indicator organisms of their host bacteria. We developed a novel real-time PCR (qPCR) assay targeting the highly conserved helicase sequence harbored in all currently known Brucella-specific lytic phages. Quality and performance tests determined a limit of detection of <1 genomic copy/µL. In raw milk artificially contaminated with Brucella microti, Izv phages were reliably detected after 39 h of incubation, indicating the presence of viable bacteria. The qPCR assay showed high stability in the milk matrix and significantly shortened the time to diagnosis when compared to traditional culture-based techniques. Hence, our molecular assay is a reliable and sensitive method to analyze phage titers, may help to reduce the hands-on time needed for the screening of potentially contaminated food, and reveals infection risks without bacterial isolation.
Spectral Induced Polarization Survey with Distributed Array System for Mineral Exploration: Case Study in Saudi Arabia
Authors: Alfouzan Fouzan A. , Alotaibi Abdulrahman M. , Cox Leif H. , Zhdanov Michael S.
Published: August 2020

The Saudi Arabian Glass Earth Pilot Project is a geophysical exploration program to explore the upper crust of the Kingdom for minerals, groundwater, and geothermal resources as well as strictly academic investigations. The project began with over 8000 km2 of green-field area. Airborne geophysics including electromagnetic (EM), magnetics, and gravity were used to develop several high priority targets for ground follow-up. Based on the results of airborne survey, a spectral induced polarization (SIP) survey was completed over one of the prospective targets. The field data were collected with a distributed array system, which has the potential for strong inductive coupling. This was examined in a synthetic study, and it was determined that with the geometries and conductivities in the field survey, the inductive coupling effect may be visible in the data. In this study, we also confirmed that time domain is vastly superior to frequency domain for avoiding inductive coupling, that measuring decays from 50 ms to 2 s allow discrimination of time constants from 1 ms to 5 s, and the relaxation parameter C is strongly coupled to intrinsic chargeability. We developed a method to fully include all 3D EM effects in the inversion of induced polarization (IP) data. The field SIP data were inverted using the generalized effective-medium theory of induced polarization (GEMTIP) in conjunction with an integral equation-based modeling and inversion methods. These methods can replicate all inductive coupling and EM effects, which removes one significant barrier to inversion of large bandwidth spectral IP data. The results of this inversion were interpreted and compared with results of drill hole set up in the survey area. The drill hole intersected significant mineralization which is currently being further investigated. The project can be considered a technical success, validating the methods and effective-medium inversion technique used for the project.
Origin of Northeast Fujian Basalts and Limitations on the Heterogeneity of Mantle Sources for Cenozoic Alkaline Magmatism across SE China: Evidence from Zircon U-Pb Dating, Petrological, Whole-Rock Geochemical, and Isotopic Studies
Authors: Zhang Guishan , Peng Ren , Qiu Hongxin , Wen Hanjie , Feng Yonggang , Chen Baoyun , Zhang Lei , Liu Shen , Liu Taotao
Published: August 2020

Cenozoic alkali basalts in Southeast (SE) China generally are genetically related to intracontinental rifting. Hence, they can be used to probe the nature of their underlying mantle sources and aid studies of the tectonic background in this region. This paper focuses on the Shanhoujian alkali basalts located in Bailing County, northeastern Fujian, SE China. We herein report their petrology, whole-rock major, and trace element geochemistry, and Sr-Nd isotopic composition and provide a new zircon U-Pb age for the basalts (~40 Ma, Eocene). These data help to constrain the petrogenesis of alkali basalts, their mantle source, and tectonic settings. The basalts are characterized by high Mg# (58.21–63.52) with Na2O/K2O > 1. MgO content is weakly correlated with CaO and Cr content but shows no correlation with Ni and Fe2O3 (total). Such features suggest that fractionation of clinopyroxene rather than olivine was important. In terms of trace elements, the alkali basalts display: (1) enrichment in La, Ce, Rb, Ba, Nb, and Ta and depletion in K, Pb, Zr, Hf, and Ti and (2) notable fractionation of light rare earth elements from heavy rare earth elements. Determined (87Sr/86Sr)i is in the range of 0.7041–0.7040 and εNd (t) is between +3.2 and +3.3. The Shanhoujian alkali basalts show a notable affinity to oceanic island basalts (OIBs) with little assimilation of crustal materials. They were derived from a pyroxenite and carbonated peridotite mantle source metasomatized by sediments carried by the subduction plate at different depths. The primary magmas of these basalts were derived from partial melting of this metasomatized mantle source during upwelling of the asthenospheric mantle as an intracontinental rift formed through extension in this part of SE China.
Progress on the Industrial Applications of Red Mud with a Focus on China
Authors: Zeng Hua , Lyu Fei , Sun Wei , Zhang Hai , Wang Li , Wang Yanxiu
Published: August 2020

Red mud (RM), also called bauxite residue, is a strong alkaline industrial waste generated during the alumina production process. The annual production of RM in China is large, but its average utilization rate is low (only 4%). High generation and low consumption make the disposal of RM mainly by stockpiling, which has caused serious heavy metal pollution and radioactive contamination. In this paper, the various industrial utilization methods of RM in China during the past 60 years have been introduced. Moreover, some recent industrial progresses were referred. The results show that RM can be widely used in building materials, valuable metals extraction, and some novel utilization methods, such as silica-calcium fertilizer, inorganic polymer material and desulfurizer. Most of the industrial utilization methods of RM have been used until now and some successfully applied to other aluminum plants, providing some feasible routes for a large amount utilization of RM. Some industrial utilization methods (such as oil well cement and calcium silicon fertilizer) have not been used due to some problems that cannot be ignored, but it provided a lot of valuable experience and was helpful for the subsequent RM utilization. Moreover, some novel and feasible RM utilization methods were proposed and successfully industrialized, which showed that RM has a broader application prospect. Many actual practices showed that the best way to safely dispose of RM was to develop technology that could consume large amounts of RM or transform it into secondary resources, which may need more time and effort.
Geochemistry of Carboniferous–Permian Coal from the Wujiawan Mine, Datong Coalfield, Northern China: Modes of Occurrence, Origin of Valuable Trace Elements, and Potential Industrial Utilization
Authors: Ma Jialiang , Xiao Lin , Zhang Ke , Jiao Yukun , Wang Zhenzhen , Li Jinxiao , Guo Wenmu , Gao Pengpeng , Qin Shenjun , Zhao Cunliang
Published: August 2020

This paper provides new geochemical data focusing on valuable elements in the coal, parting, and floor samples in the No. 5 coal seam of the Taiyuan Formation from the Wujiawan mine, Datong coalfield, northern China. The minerals mainly consist of kaolinite, calcite, and pyrite, as well as trace amounts of quartz and illite. The No. 5 coal is enriched in Li, Ga, high field strength elements (HFSEs), and rare earth elements and yttrium (REY) when compared with world hard coals. Of particular interest is the high average concentration of Li (67.66 μg/g), which is around seven times higher than the value for world hard coals. Lithium, Ga, and HFSEs have strong inorganic affinities, whereas REY have organic affinities. The main carrier of Li, Ga, and HFSEs is aluminosilicate minerals, while REY appear to occur with organophosphorus. These HFSEs are enriched, both in the parting and in the adjacent coal samples. This suggests that these elements are likely to leach out during the diagenetic process. The distribution patterns of REY, along with the ratio of Al2O3/TiO2 and the figure of Zr/TiO2 vs. Nb/Y are suggestive of their derivation from felsic parent material. In the northern and eastern part of the Datong coalfield, there are several regions where the Li content is higher than the mineable grade, in particular in the northern Datong coalfield where there is a mine with an Li content of 294.6 μg/g. This is significantly higher than the mineable grade. Therefore, there is a potential for financially viable recovery of Li in these coals of the Datong coalfield.
Capsaicin Causes Vasorelaxation of Rat Aorta through Blocking of L-type Ca2+ Channels and Activation of CB1 Receptors
Authors: Andrade Felipa , Rangel-Sandoval Cinthia , Rodríguez-Hernández Alejandrina , López-Dyck Evelyn , Elizalde Alejandro , Virgen-Ortiz Adolfo , Bonales-Alatorre Edgar , Valencia-Cruz Georgina , Sánchez-Pastor Enrique
Published: August 2020

The aim of this work was to determine whether Capsaicin may exert a vascular regulation through the activation of CB1 and/or CB2 receptors causing vasorelaxation in the rat aorta. Our results show the location of TRPV1 mainly in the endothelial and smooth muscle cells membrane. Nevertheless, Capsaicin caused vasorelaxation of this artery through a mechanism independent of TRPV1, since the specific antagonists Capsazepine and SB-366791 did not block the effect of Capsaicin. Because the significant expression of CB1 and CB2 receptors has been previously reported in the rat aorta, we used antagonists for these two receptors prior to the addition of Capsaicin. In these experiments, we found that the inhibition of CB1 using AM281, decreases the vasorelaxant effect caused by Capsaicin. On the other hand, the vasorelaxant effect is not altered in the presence of the CB2 receptor antagonist AM630. Furthermore, a partial decrease of the effect of Capsaicin was also seen when L-type calcium channels are blocked. A complete block of Capsaicin-induced vasorelaxation was achieved using a combination of Verapamil and AM281. In accordance to our results, Capsaicin-induced vasorelaxation of the rat aorta is neither dependent of TRPV1 or CB2 receptors, but rather it is strongly suggested that a tandem mechanism between inactivation of L-type calcium channels and the direct activation of CB1 receptors is involved. These findings are supported by CB1 docking simulation which predicted a binding site on CB1 receptors for Capsaicin.
Fish-Based Baby Food Concern—From Species Authentication to Exposure Risk Assessment
Authors: Pappalardo Anna Maria , Copat Chiara , Raffa Alessandra , Rossitto Luana , Grasso Alfina , Fiore Maria , Ferrante Margherita , Ferrito Venera
Published: August 2020

In this work, two different but complementary approaches were used to evaluate the reliability of fish-based baby foods as a source of safe nourishment for babies. More specifically, barcoding analysis based on the Cytochrome Oxidase I sequences was used for fish species authentication and an analysis of metal/metalloid levels was performed to estimate the exposure risk assessment derived from consumption of selected fish-based baby food in infants and toddlers. COI DNA barcoding revealed that in three samples the species detected did not match the common name of the species shown on the label. In particular, G. chalcogrammus and M. australis were found in place of M. merluccius and O. mykiss was found in place of S. salar. The analysis of exposure risk assessment indicated a low risk for developing chronic systemic and carcinogenic effects in infants and toddler, under an exposure scenario based on daily consumption of a single box of fish-based baby food. However, it is important to highlight that in order to provide a comprehensive risk assessment it would be important to supplement the levels of exposure resulting from the total diet. Overall, our results suggest that more attention should be paid by authorities to ensure the safety of food for infants and toddlers.
Competitive Interaction of Phosphate with Selected Toxic Metals Ions in the Adsorption from Effluent of Sewage Sludge by Iron/Alginate Beads
Authors: Siwek Hanna , Pawelec Krzysztof
Published: August 2020

Wastewater is characterized by a high content of phosphate and toxic metals. Many studies have confirmed the sorption affinity of alginate adsorbents for these ions. In this study, the adsorption of phosphate from effluent of sewage sludge on biodegradable alginate matrices cross-linked with Fe3+ ions (Fe_Alg) was investigated. Kinetics and adsorption isotherms were tested in laboratory conditions in deionized water (DW_P) and in the effluent (SW_P), and in the same solutions enriched in toxic metals ions—Cu2+, Cd2+, Pb2+, and Zn2+ (DW_PM and SW_PM). Batch experiments were performed by changing the concentration of phosphate at constant metal concentration. Kinetics experiments indicated that the pseudo-second-order model displayed the best correlation with adsorption kinetics data for both metals and phosphate. The Freundlich equation provided the best fit with the experimental results of phosphate adsorption from DW_P and DW_PM, while the adsorption from SD_P and SD_PM was better described by the Langmuir equation. For tested systems, the affinity of the Fe_Alg for metal ions was in the following decreasing order: Pb2+ > Cu2+ > Cd2+ > Zn2+ in DW_PM, and Pb2+ > Cu2+ > Cd2+ > Zn2+ in SW_PM. The metals’ enrichment of the DW_P solution increased the affinity of Fe_Alg beads relating to phosphate, while the addition of the metals of the SW_P solution decreased this affinity.
Bichromatic Photoassociation Spectroscopy for the Determination of Rotational Constants of Cs2

0
u
+

Long-Range State below the 6S1/2 + 6P1/2 Asymptote
Authors: Wu Jizhou , Ma Jie , Li Yuqing , Liu Wenliang , Li Peng , Sovkov Vladimir B.
Published: August 2020

This article demonstrates new observation of the high-resolution ro-vibrational bichromatic photoassociation spectra (BPAS) of Cs2 in the 0u+ long-range state below the asymptotes 6S1/2 + 6P1/2. By combining with a modulation spectroscopic technique, precise references of the frequency differences have been engineered through the BPAS, with which the rotational constants of low-lying vibrational levels of the Cs20u+ long-range state have been accurately determined by fitting the frequency differences to the non-rigid-rotor model. The rotational constants for the newly observed seven ro-vibrational levels are summarized and disagreement for the level ῦ = 498 is clarified. The rotational constants of different vibrational levels demonstrate strong perturbations of the related energy structures. A simple analysis is performed and shows good agreement with experimental results.