bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of Methyl Orange-Cetyltrimethyl Ammonium Bromide Interaction by Conductivity Method in Methanol-Water Mixed Solvent Media
Authors: Khanal Manoj
Published: June 2017

The interaction of an anionic dye (Methyl Orange) with cationic surfactant (Cetyltrimethylammonium Bromide, CTAB) in the series of solvent containing variable compositions of methanol-water mixture (10%, 20%, 30% and 40%) was studied at room temperature (31±2oC). Conductivity measurements were done for the investigation of interaction of dyes. The specific conductance of 6.58x10-5 M to 59.22x10-5M surfactant (CTAB) and these surfactants with 1.008x10-3M dye (MO) mixtures were noted at room temperature. A theoretical model was used to calculate conductance ratio from the data of measured specific conductance values. Values of conductance ratio of CTAB-MO mixtures were found to be all less than 1 which indicated that CTAB-MO dye -surfactant mixture exert significant influence on the degree of interaction.Int. J. Appl. Sci. Biotechnol. Vol 5(2): 172-179
The Assessment of Nurses’ Knowledge and Practices about Fluid and Electrolytes Monitoring and Administration among Cardiac Surgery Patients: A Case of Punjab Institute of Cardiology
Authors: Aslam Shumaila , Afzal Muhammad , Kousar Robina , Waqas Ali , Gilani Syed Amir
Published: June 2017

Background:  In order to maintain the health, the homeostasis dynamics and balance process is most important. Among the critically ill cardiac surgery patients the homeostasis is imbalance and results in insufficient tissues perfusion and many organs of the body lead to the failure condition. It is necessary to monitor actively the intake and output during electrolytes administration in the cardiac surgery patients.Objectives: To assess the knowledge and practice of the registered nurses about fluid and electrolytes monitoring and administration in the cardiac surgery patients. Methodology: This study is of descriptive and cross sectional study design. The questioner of knowledge and practices regarding fluid and electrolytes administration was adopted from the article of Vijayan (Vijayan, 2011) which is based on 5-point Likert scale. The data was collected from 208 nurses of Punjab Institute of Cardiology, Lahore, Pakistan. Conclusion: The findings of the current study show that the nurses have poor knowledge and practices of nurses, so, public hospital’s administration should focus on the strategies and activities which become helpful to increase the knowledge and practices of nurses regarding fluid and electrolytes administration. Int. J. Appl. Sci. Biotechnol. Vol 5(2): 208-215
Accessibility Scenario Analysis of a Hypothetical Future Transit Network: Social Equity Implications of a General Transit Feed Specification–Based Sketch Planning Tool
Authors: Guthrie Andrew , Fan Yingling , Das Kirti Vardhan
Published: June 2017

Accessibility analysis can have important implications for understanding social equity in transit planning. The emergence and the increasingly broad acceptance of the general transit feed specification (GTFS) format for transit route, stop, and schedule data have revolutionized transit accessibility research by providing researchers with a convenient, publicly available source of data interoperable with common geographic information system (GIS) software. Existing approaches to GTFS-based transit analysis, however, focus on currently operating transit systems. With major transit expansions across the nation and around the world increasing in number and ambition, understanding the accessibility impacts of proposed projects in their early planning stages is crucial to achieving the greatest possible social benefit from these massive public investments. This paper describes the development of a hypothetical transit network based on current GTFS data and proposed 2040 transit improvements for the Twin Cities region of Minneapolis–Saint Paul, Minnesota, as well as its use as a sketch planning tool in exploring the proposed system’s impacts on access to job vacancies from historically disadvantaged areas. This research demonstrates the importance of accessibility analysis in planning a transit system that increases opportunity for marginalized workers and concludes by calling for broader, easier access to accessibility analysis for practitioners and community groups to refine the early stages of the transit planning process and democratize an increasingly crucial transit planning tool.
Decision Support Framework for Transit-Oriented Development Projects
Authors: Strong Kelly C. , Ozbek Mehmet E. , Sharma Avi , Akalp Duygu
Published: June 2017

Although the use of personal automobiles in the United States continues to grow, there are increasing concerns about the possible effects of automobile-dependent transportation, including environmental concerns, the reduction in the quality of life caused by long commutes, and a decline of community and societal interactions. These concerns have led to discussions on the revitalization of public transportation systems. To counter the problem of congestion resulting from modern urbanization, urban planners have developed the idea of transit-oriented development (TOD), designed to encourage the use of public transit and the creation of pedestrian-friendly environments. The plethora of literature on this subject is no surprise, considering the issues of revitalization of community living and affordable housing and related factors directly influencing and getting influenced by TOD. Notwithstanding the abundance of literature on TOD, the literature review uncovered few studies that somewhat address the important question, “How can a transit agency choose between alternative TOD sites to develop or build?” Given this, the purpose of this research is to develop a decision support framework that can be used by different transit agencies when choosing a TOD site to develop or build by incorporating and assessing unique success factors and their weights. This research uses a multiple-criteria decision-making tool called the analytic hierarchy process in developing that framework. The paper presents two implementation examples that demonstrate the feasibility of the developed decision support framework for both a large, urban transit district and a smaller, more rural transit agency.
Clinical Profile of Acute Pancreatitis in Children in a Tertiary Level Hospital of Bangladesh
Authors: Musabbir Nadira , Karim ASM Bazlul , Mazumder Md Wahiduzzaman , Sultana Kaniz , Anwar Syeda Afria , Haque Md Ariful , Hussain Faika
Published: June 2017

Background: Acute pancreatitis is an acute inflammatory condition of the pancreas that may extend to local and distant extrapancreatic tissues. The incidence of acute pancreatitis in children has increased significantly in the past two decades. It can be associated with severe morbidity and mortality. It should be considered in every child with unexplained acute abdominal pain.Objectives: To observe the clinical, biochemical and imaging profiles of acute pancreatitis in children.Methods: It was a cross-sectional study conducted at the Department of Pediatric Gastroenterology & Nutrition of Bangabandhu Sheikh Mujib Medical University, Dhaka from January 2014 through June 2015. A total of 50 cases of acute pancreatitis were included in this study. The diagnosis of acute pancreatitis was based on diagnostic criteria of acute pancreatitis made by INSPPIRE group (If a child had any 2 of the 3 criteria: the abdominal pain compatible with acute pancreatitis, elevated serum amylase and /or lipase level more than three times of upper limit of normal, imaging findings compatible with acute pancreatitis). Clinical characteristics, laboratory and imaging profile of the cases, complications were studied.Results: Among 50 cases, male were 46% and male female ratio was 0.8:1. Mean age at presentation was 10.2 ± 3.2 years. Forty eight (96%) patients had abdominal pain which was severe agonizing in 81.3% cases. The common location of pain was in epigastric region (77%). Pain radiating to back in 22.9% patients. Mean duration of pain was 6.6 ± 4.4 days before hospital admission. Vomiting was present in 72% patients followed by fever (30%). Two (4%) patients had jaundice. Ascites was noted in 12% patients and abdominal mass in 6% patients. Out of 50 cases of AP, biliary sludge was associated in 6% patients, biliary ascariasis in 4%, choledochal cyst in 2% and gallbladder stone in 2% patients. But in this study, 4% patients had Wilson disease. Laboratory tests showed leukocytosis in 28% patients, high serum amylase and lipase level in 56% and 58% patients respectively. Postive findings in ultrasonogram were present in 66% patients. In the present study, hypocalcemia was found in 38% patients, pseudocyst in 6% and pancreatic necrosis in 2% patients.Conclusion: Although acute pancreatitis may present with varieties of clinical feature, the most common one is abdominal pain and common location of pain is in epigastric region. For confirmation of clinically diagnosed pancreatitis, both serum amylase and lipase level and abdominal ultrasound are useful tools.Bangladesh J Child Health 2016; VOL 40 (3) :160-165
Cross-validation of the Student Perceptions of Team-Based Learning Scale in the United States
Authors: Lein Donald H. , Lowman John D. , Eidson Christopher A. , Yuen Hon K.
Published: June 2017

Purpose: The purpose of this study was to cross-validate the factor structure of the previously developed Student Perceptions of Team-Based Learning (TBL) Scale among students in an entry-level doctor of physical therapy (DPT) program in the United States.Methods: Toward the end of the semester in 2 patient/client management courses taught using TBL, 115 DPT students completed the Student Perceptions of TBL Scale, with a response rate of 87%. Principal component analysis (PCA) and confirmatory factor analysis (CFA) were conducted to replicate and confirm the underlying factor structure of the scale. Results: Based on the PCA for the validation sample, the original 2-factor structure (preference for TBL and preference for teamwork) of the Student Perceptions of TBL Scale was replicated. The overall goodness-of-fit indices from the CFA suggested that the original 2-factor structure for the 15 items of the scale demonstrated a good model fit (comparative fit index, 0.95; non-normed fit index/Tucker-Lewis index, 0.93; root mean square error of approximation, 0.06; and standardized root mean square residual, 0.07). The 2 factors demonstrated high internal consistency (alpha= 0.83 and 0.88, respectively). DPT students taught using TBL viewed the factor of preference for teamwork more favorably than preference for TBL.Conclusion: Our findings provide evidence supporting the replicability of the internal structure of the Student Perceptions of TBL Scale when assessing perceptions of TBL among DPT students in patient/client management courses.
Sampietrini Stone Pavements: Distress Analysis Using Pavement Condition Index Method
Authors: Zoccali Pablo , Loprencipe Giuseppe , Galoni Andrea
Published: June 2017

In several Italian cities, it is possible to find historical pavements such as the Sampietrini pavements, which are mainly located in the center of the city of Rome. The Sampietrini pavement is a particular road surface paved in natural stone with irregular sharp elements that are assembled by hand with the evident not plan effect. Because of their peculiarities, they are not suitable for streets where high speed is allowed. In many cases, high vibration and noise levels due to road traffic traveling on Sampietrini pavements are caused by inadequate maintenance, which is also affected by the absence of specific evaluation criteria regarding surface conditions and performances of Sampietrini pavements. It is not possible, in fact, to adopt common approaches developed to be used for flexible and rigid pavements, because they present completely different features and distresses. In this paper, to overpass this problem, a new evaluation criterion based on Pavement Condition Index (PCI) method established for block pavements is proposed. Furthermore, to fully characterize this kind of pavements, other analyses, i.e., International Roughness Index (IRI) and comfort level evaluation based on ISO 2631 standard, were also carried out. The results showed a good correlation between PCI and IRI approaches (R2 = 0.82), also highlighting that new or reconstructed Sampietrini pavements present not negligible roughness level. This aspect was also confirmed estimating the comfort level perceived by users traveling at several speeds (≤50 km/h). Finally, speed related threshold values to be adopted for PCI and IRI methods are proposed. The proposed method can be implemented by pavement managers in a PMS ad hoc for stone block paving and thus, it can be integrated with other equivalents methods of visual inspection based on PCI.
Functional Amyloids in Reproduction
Authors: Hewetson Aveline , Do Hoa Quynh , Myers Caitlyn , Muthusubramanian Archana , Sutton Roger Bryan , Wylie Benjamin J. , Cornwall Gail A.
Published: June 2017

Amyloids are traditionally considered pathological protein aggregates that play causative roles in neurodegenerative disease, diabetes and prionopathies. However, increasing evidence indicates that in many biological systems nonpathological amyloids are formed for functional purposes. In this review, we will specifically describe amyloids that carry out biological roles in sexual reproduction including the processes of gametogenesis, germline specification, sperm maturation and fertilization. Several of these functional amyloids are evolutionarily conserved across several taxa, including human, emphasizing the critical role amyloids perform in reproduction. Evidence will also be presented suggesting that, if altered, some functional amyloids may become pathological.
Concrete Damage in Field Conditions and Protective Sealer and Coating Systems
Authors: Safiuddin Md.
Published: June 2017

Concrete structures undergo different forms of damage during their service life. The aim of this study is to identify the most common forms of concrete damage in field conditions. A number of concrete elements and structures were visually inspected and the damage was photographed. The damage phenomena covered are delamination, popout, spalling, scaling, drying shrinkage cracking, corrosion-induced cracking, map cracking, rust and salt stains, discoloration, and mold and moss growths. The probable reasons for these damage phenomena are discussed in this study. Moreover, this study highlights how concrete structures can be protected from the aforementioned forms of damage by using sealer and coating systems as surface treatments. Different sealer and coating systems, emphasizing their selection for specific uses, installation on concrete substrate, effects on concrete durability, performance criteria, and performance evaluation methods have been discussed. Furthermore, some recommendations are given to improve the performance of concrete sealer and coating systems for the protection of concrete structures in field conditions.
Energy-Related CO2 Emissions Forecasting Using an Improved LSSVM Model Optimized by Whale Optimization Algorithm
Authors: Zhao Haoran , Guo Sen , Zhao Huiru
Published: June 2017

Accurate and reliable forecasting on energy-related carbon dioxide (CO2) emissions is of great significance for climate policy decision making and energy planning. Due to the complicated nonlinear relationships of CO2 emissions with its driving forces, the accurate forecasting for CO2 emissions is a tedious work, which is an important issue worth studying. In this study, a novel CO2 emissions prediction method is proposed which employs the latest nature-enlightened optimization method, named the Whale optimization algorithm (WOA), to search the optimized values of two parameters of LSSVM (least squares support vector machine), namely the WOA-LSSVM model. Meanwhile, the driving forces of CO2 emissions including GDP (gross domestic product), energy consumption and population are chosen to be the import variables of the proposed WOA-LSSVM method. Taking China’s CO2 emissions as an instance, the effectiveness of WOA-LSSVM-based CO2 emissions forecasting is verified. The comparative analysis results indicate that the WOA-LSSVM model is significantly superior to other selected models, namely FOA (fruit fly optimization algorithm)-LSSVM, LSSVM, and OLS (ordinary least square) models in terms of CO2 emissions forecasting. The proposed WOA-LSSVM model has the potential to effectively improve the accuracy of CO2 emissions forecasting. Meanwhile, as a new nature-enlightened heuristic optimization algorithm, the WOA has the prospect for wide application.
Measurement of Within-Season Tree Height Growth in a Mixed Forest Stand Using UAV Imagery
Authors: Dempewolf Jan , Nagol Jyoteshwar , Hein Sebastian , Thiel Carsten , Zimmermann Reiner
Published: June 2017

Tree height growth measurements at monthly and annual time scales are important for calibrating and validating forest growth models, forest management and studies of forest ecology and biophysical processes. Previous studies measured the terminal growth of individual trees or forest stands at annual or decadal time scales. Short-term, within-season measurements, however, are largely unavailable due to technical and practical limitations. Here, we describe a novel approach for measuring within-season tree height growth using a time series of co-registered digital surface models obtained with a low-cost unmanned aerial vehicle in combination with ground control plates and Structure from Motion data processing. The test site was a 2-hectare temperate mixed forest stand of varying age and successional stage in central Europe. Our results show median growth rates between 27 May and 19 August of 68 cm for Norway spruce, 93 cm for Scots pine, 106 cm for Silver birch and 26 cm for European beech. The results agree well with published field observations for these species. This study demonstrates the capability of inexpensive, increasingly user-friendly and versatile UAV systems for measuring tree height growth at short time scales, which was not previously possible, opening up new avenues for investigation and practical applications in forestry and research.
Silicate-Promoted Phosphorylation of Glycerol in Non-Aqueous Solvents: A Prebiotically Plausible Route to Organophosphates
Authors: Gull Maheen , Cafferty Brian , Hud Nicholas , Pasek Matthew
Published: June 2017

Phosphorylation reactions of glycerol were studied using different inorganic phosphates such as sodium phosphate, trimetaphosphate (a condensed phosphate), and struvite. The reactions were carried out in two non-aqueous solvents: formamide and a eutectic solvent consisting of choline-chloride and glycerol in a ratio of 1:2.5. The glycerol reacted in formamide and in the eutectic solvent with phosphate to yield its phosphorylated derivatives in the presence of silicates such as quartz sand and kaolinite clay. The reactions were carried out by heating glycerol with a phosphate source at 85 °C for one week and were analyzed by 31P-nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS). The yield of the phosphorylated glycerol was improved by the presence of silicates, and reached 90% in some experiments. Our findings further support the proposal that non-aqueous solvents are advantageous for the prebiotic synthesis of biomolecules, and suggest that silicates may have aided in the formation of organophosphates on the prebiotic earth.
On the Transition from Static to Dynamic Boundary Friction of Lubricated PEEK for a Spreading Adhesive Contact by Macroscopic Oscillatory Tribometry
Authors: Abdel-Wahed Sherif Ahmed , Koplin Christof , Jaeger Raimund , Scherge Matthias
Published: June 2017

The tribology of lubricated poly(ether ether ketone) (PEEK)—steel tribosystems was investigated in the static and boundary friction regime. Pentaerythrite ester and trimellitic acid ester were used as lubricants. The lubricants differed in their molecular structure but showed only minor differences in their rheological and cohesive energetic properties. In order to investigate the effect of the lubricants on static and dynamic friction, macroscopic oscillatory tribometry experiments and gliding experiments were carried out. The surface and interfacial energies of PEEK, lubricant, and steel, which can be used to determine e.g. the spreading tendency of the lubricant, were employed to explain the tribological characteristics of the system. The gliding experiments exhibited a velocity dependence of the coefficient of friction which indicates a “rate-and-state” type of friction. Trimellitic acid ester resulted in the lowest static and dynamic friction values due to its high tendency to spread. A different static friction behavior was found for pentaerythrite ester, which indicates a possible lubricant-induced mobilization of the polymer surface chains. This finding was supported by an increased interaction energy measured by contact angle measurements. The macroscopic oscillatory tribometry was shown to be a unique and precise method to investigate the transition from static to dynamic friction and to quantify macroscopic adhesive friction. Both types of experiments used a polished steel surface as a frictional partner, which favors the adhesive component of friction. However, the results for the lubricated contacts of polished steel surfaces in the boundary lubrication regime can give some insight into effects of the polymer transfer to rough steel surfaces in the mixed lubrication regime.
The Influence of Laser Welding on the Mechanical Properties of Dual Phase and Trip Steels
Authors: Evin Emil , Tomáš Miroslav
Published: June 2017

Nowadays, a wide range of materials is used for car body structures in order to improve both the passengers’ safety and fuel consumption. These are joined by laser welding and solid state fiber lasers being used more and more in present. The article is focused on the research of laser welding influence on the mechanical and deformation properties, microstructure and microhardness of advanced high-strength steels: high-strength low-alloyed steel HC340LA, dual phase steel HCT600X and multi-phase residual austenite steel RAK40/70. The proper welding parameters have been found based on weld quality evaluation. The specimens for tensile test with longitudinal laser weld were used to measure mechanical and deformation properties. Microstructure and microhardness of laser welds were evaluated in the base metal, heat affected zone and fusion zone. The higher values of strength and lower ones for deformation properties of laser-welded materials have been found for dual and multi-phase steel. The microhardness strongly depends on the carbon equivalent of steel. Deformation properties are more sensitive than strength properties to the change of microstructure in the fusion zone and heat affected zone.
Electroceutical Approach for Impairing the Motility of Pathogenic Bacterium Using a Microfluidic Platform
Authors: Berthelot Ryan , Doxsee Kristina , Neethirajan Suresh
Published: June 2017

Electrotaxis, or galvanotaxis, refers to the migration pattern of cells induced in response to electrical potential. Electrotaxis has not been explored in detail in bacterial cells; information regarding the impact of current on pathogenic bacteria is severely lacking. Using microfluidic platforms and optical microscopy, we designed a series of single- and multi-cue experiments to assess the impact of varying electrical currents and acetic acid concentrations on bacterial motility dynamics in pathogenic multi-drug resistant (MDR) strains of Pseudomonas aeruginosa and Escherichia coli. The use of the microfluidic platform allows for single-cue experiments where electrical current is supplied at a range that is biocidal to bacteria and multi-cue experiments where acetic acid is combined with current to enhance disinfection. These strategies may offer substantial therapeutic benefits, specifically for the treatment of biofilm infections, such as those found in the wound environment. Our results showed that an application of current in combination with acetic acid has profound inhibitory effects on MDR strains of P. aeruginosa and E. coli, even with brief applications. Specifically, E. coli motility dynamics and cell survival were significantly impaired starting at a concentration of 0.125 mA of direct current (DC) and 0.31% acetic acid, while P. aeruginosa was impaired at 0.70 mA and 0.31% acetic acid. As these strains are relevant wound pathogens, it is likely that this strategy would be effective against similar strains in vivo and could represent a new approach to hasten wound healing.
Research on the Drilling Performance of a Helical Point Micro Drill with Different Geometry Parameters
Authors: Liang Zhiqiang , Zhang Suyan , Wang Xibin , Guo Haixin , Zhou Tianfeng , Jiao Li , Yan Pei
Published: June 2017

During the micro-drilling process of stainless steel, the wear, fracture, and breakage of the micro-drill easily occur. Micro-drill geometry parameters have significant influence on the drilling performance of the micro-drill. Nowadays, the helical point micro-drill is proposed and its improved drilling performance is validated by some researchers. In this study, to analyze the effect of geometry parameters of the helical point micro-drill on drilling performance, the mathematical models of the helical flank and ground flute are proposed, and the cutting lip shape, rake angle, and uncut chip thickness are calculated using MATLAB software. Then, based on the orthogonal tests, nine kinds of micro-drills with different point angles, web thicknesses, and helix angles are fabricated using a six-axis CNC tool grinder, and micro-drilling experiments on 1Cr18Ni9Ti stainless steel are carried out. The drilling force, the burr height, and the hole wall quality are measured and observed. The results show that the point angle is the main contributing factor for the thrust force and burr height, and the web thickness is the main contributing factor for the micro hole wall quality. The increased point angle offers a larger thrust force, but gives rise to a smaller exit burr. A larger web thickness leads to a larger thrust force and burr height, and results in a poor surface quality. With the helix angle increased, the thrust force and burr height decreases, and the surface quality of micro-hole improves. The geometry parameters with a point angle 70°, a point angle of 40°, and web thickness ratio of 0.2 can used to improve the drilling performance of the helical point micro-drill.
Glycosylation of Recombinant Antigenic Proteins from Mycobacterium tuberculosis: In Silico Prediction of Protein Epitopes and Ex Vivo Biological Evaluation of New Semi-Synthetic Glycoconjugates
Authors: Bavaro Teodora , Tengattini Sara , Piubelli Luciano , Mangione Francesca , Bernardini Roberta , Monzillo Vincenzina , Calarota Sandra , Marone Piero , Amicosante Massimo , Pollegioni Loredano , Temporini Caterina , Terreni Marco
Published: June 2017

Tuberculosis is still one of the most deadly infectious diseases worldwide, and the use of conjugated antigens, obtained by combining antigenic oligosaccharides, such as the lipoarabinomannane (LAM), with antigenic proteins from Mycobacterium tuberculosis (MTB), has been proposed as a new strategy for developing efficient vaccines. In this work, we investigated the effect of the chemical glycosylation on two recombinant MTB proteins produced in E. coli with an additional seven-amino acid tag (recombinant Ag85B and TB10.4). Different semi-synthetic glycoconjugated derivatives were prepared, starting from mannose and two disaccharide analogs. The glycans were activated at the anomeric position with a thiocyanomethyl group, as required for protein glycosylation by selective reaction with lysines. The glycosylation sites and the ex vivo evaluation of the immunogenic activity of the different neo-glycoproteins were investigated. Glycosylation does not modify the immunological activity of the TB10.4 protein. Similarly, Ag85B maintains its B-cell activity after glycosylation while showing a significant reduction in the T-cell response. The results were correlated with the putative B- and T-cell epitopes, predicted using a combination of in silico systems. In the recombinant TB10.4, the unique lysine is not included in any T-cell epitope. Lys30 of Ag85B, identified as the main glycosylation site, proved to be the most important site involved in the formation of T-cell epitopes, reasonably explaining why its glycosylation strongly influenced the T-cell activity. Furthermore, additional lysines included in different epitopes (Lys103, -123 and -282) are also glycosylated. In contrast, B-cell epitopic lysines of Ag85B were found to be poorly glycosylated and, thus, the antibody interaction of Ag85B was only marginally affected after coupling with mono- or disaccharides.
LXRα Regulates Hepatic ChREBPα Activity and Lipogenesis upon Glucose, but Not Fructose Feeding in Mice
Authors: Fan Qiong , Nørgaard Rikke , Bindesbøll Christian , Lucas Christin , Dalen Knut , Babaie Eshrat , Itkonen Harri , Matthews Jason , Nebb Hilde , Grønning-Wang Line
Published: June 2017

Liver X receptors (LXRα/β) and carbohydrate response element-binding proteins (ChREBPα/β) are key players in the transcriptional control of hepatic de novo lipogenesis. LXRα/β double knockout (LXRα−/−/β−/−) mice have reduced feeding-induced nuclear O-linked N-acetylglucosamine (O-GlcNAc) signaling, ChREBPα activity, and lipogenic gene expression in livers, suggesting important roles for LXRs in linking hepatic glucose utilization to lipid synthesis. However, the role of LXRs in fructose-induced ChREBP activation and lipogenesis is currently unknown. In this study, we studied the effects of high fructose or high glucose feeding on hepatic carbohydrate metabolism and lipogenic gene expression in livers from fasted (24 h) and fasted-refed (12 h) wild type and LXRα knockout (LXRα−/−) mice. Hepatic lipogenic gene expression was reduced in glucose fed, but not fructose fed LXRα−/− mice. This was associated with lower expression of liver pyruvate-kinase (L-pk) and Chrebpβ, indicating reduced ChREBPα activity in glucose fed, but not fructose fed mice. Interestingly, ChREBP binding to the L-pk promoter was increased in fructose fed LXRα−/− mice, concomitant with increased glucose-6-phosphatase (G6pc) expression and O-GlcNAc modified LXRβ, suggesting a role for LXRβ in regulating ChREBPα activity upon fructose feeding. In conclusion, we propose that LXRα is an important regulator of hepatic lipogenesis and ChREBPα activity upon glucose, but not fructose feeding in mice.
Evaluation of Satellite-Based Rainfall Estimates and Application to Monitor Meteorological Drought for the Upper Blue Nile Basin, Ethiopia
Authors: Bayissa Yared , Tadesse Tsegaye , Demisse Getachew , Shiferaw Andualem
Published: June 2017

Drought is a recurring phenomenon in Ethiopia that significantly impacts the socioeconomic sector and various components of the environment. The overarching goal of this study is to assess the spatial and temporal patterns of meteorological drought using a satellite-derived rainfall product for the Upper Blue Nile Basin (UBN). The satellite rainfall product used in this study was selected through evaluation of five high-resolution products (Climate Hazards Group InfraRed Precipitation with Stations (CHIRPS) v2.0, Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks (PERSIANN), African Rainfall Climatology and Time-series (TARCAT) v2.0, Tropical Rainfall Measuring Mission (TRMM) and Africa Rainfall Estimate Climatology version 2 [ARC 2.0]). The statistical performance measuring techniques (i.e., Pearson correlation coefficient (r), mean error (ME), root mean square error (RMSE), and Bias) were used to evaluate the satellite rainfall products with the corresponding ground observation data at ten independent weather stations. The evaluation was carried out for 1998–2015 at dekadal, monthly, and seasonal time scales. The evaluation results of these satellite-derived rainfall products show there is a good agreement (r > 0.7) of CHIRPS and TARCAT rainfall products with ground observations in majority of the weather stations for all time steps. TARCAT showed a greater correlation coefficient (r > 0.70) in seven weather stations at a dekadal time scale whereas CHIRPS showed a greater correlation coefficient (r > 0.84) in nine weather stations at a monthly time scale. An excellent score of Bias (close to one) and mean error was observed in CHIRPS at dekadal, monthly and seasonal time scales in a majority of the stations. TARCAT performed well next to CHIRPS whereas PERSSIAN presented a weak performance under all the criteria. Thus, the CHIRPS rainfall product was selected and used to assess the spatial and temporal variability of meteorological drought in this study. The 3-month Z-Score values were calculated for each grid and used to assess the spatial and temporal patterns of drought. The result shows that the known historic drought years (2014–2015, 2009–2010, 1994–1995 and 1983–1984) were successfully indicated. Moreover, severe drought conditions were observed in the drought prone parts of the basin (i.e., central, eastern and southeastern). Hence, the CHIRPS rainfall product can be used as an alternative source of information in developing the grid-based drought monitoring tools for the basin that could help in developing early warning systems.
Biooxidation of Ciguatoxins Leads to Species-Specific Toxin Profiles
Authors: Ikehara Tsuyoshi , Kuniyoshi Kyoko , Oshiro Naomasa , Yasumoto Takeshi
Published: June 2017

Ciguatoxins (CTXs) contaminate fish worldwide and cause the foodborne illness ciguatera. In the Pacific, these toxins are produced by the dinoflagellate Gambierdiscus toxicus, which accumulates in fish through the food chain and undergoes oxidative modification, giving rise to numerous analogs. In this study, we examined the oxidation of CTXs in vitro with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using reference toxins, and found that CTX4A, CTX4B, and CTX3C, which are produced by the alga, are oxidized to the analogs found in fish, namely CTX1B, 52-epi-54-deoxyCTX1B, 54-deoxyCTX1B, 2-hydroxyCTX3C, and 2,3-dihydroxyCTX3C. This oxidation was catalyzed by human CYP3A4, fish liver S9 fractions, and microsomal fractions prepared from representative ciguateric fishes (Lutjanus bohar, L. monostigumus, and Oplegnathus punctatus). In addition, fish liver S9 fractions prepared from non-ciguateric fishes (L. gibbus and L. fulviflamma) in Okinawa also converted CTX4A and CTX4B to CTX1B, 54-deoxyCTX1B, and 52-epi-54-deoxyCTX1B in vitro. This is the first study to demonstrate the enzymatic oxidation of these toxins, and provides insight into the mechanism underlying the development of species-specific toxin profiles and the fate of these toxins in humans and fish.
Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method Coupled with Dispersive Solid-Phase Extraction for Simultaneous Quantification of Eight Paralytic Shellfish Poisoning Toxins in Shellfish
Authors: Yang Xianli , Zhou Lei , Tan Yanglan , Shi Xizhi , Zhao Zhiyong , Nie Dongxia , Zhou Changyan , Liu Hong
Published: June 2017

In this study, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for simultaneous determination of eight paralytic shellfish poisoning (PSP) toxins, including saxitoxin (STX), neosaxitoxin (NEO), gonyautoxins (GTX1–4) and the N-sulfo carbamoyl toxins C1 and C2, in sea shellfish. The samples were extracted by acetonitrile/water (80:20, v/v) with 0.1% formic and purified by dispersive solid-phase extraction (dSPE) with C18 silica and acidic alumina. Qualitative and quantitative detection for the target toxins were conducted under the multiple reaction monitoring (MRM) mode by using the positive electrospray ionization (ESI) mode after chromatographic separation on a TSK-gel Amide-80 HILIC column with water and acetonitrile. Matrix-matched calibration was used to compensate for matrix effects. The established method was further validated by determining the linearity (R2 ≥ 0.9900), average recovery (81.52–116.50%), sensitivity (limits of detection (LODs): 0.33–5.52 μg·kg−1; limits of quantitation (LOQs): 1.32–11.29 μg·kg−1) and precision (relative standard deviation (RSD) ≤ 19.10%). The application of this proposed approach to thirty shellfish samples proved its desirable performance and sufficient capability for simultaneous determination of multiclass PSP toxins in sea foods.
Occurrence of Regulated Mycotoxins and Other Microbial Metabolites in Dried Cassava Products from Nigeria
Authors: Abass Adebayo , Awoyale Wasiu , Sulyok Michael , Alamu Emmanuel
Published: June 2017

Dried cassava products are perceived as one of the potential sources of mycotoxin ingestion in human foods. Processing either contributes to the reduction of toxins or further exposes products to contamination by microorganisms that release metabolic toxins into the products. Thus, the prevalence of microbial metabolites in 373 processed cassava products was investigated in Nigeria. With the use of liquid chromatography tandem-mass spectrometry (LC-MS/MS) for the constituent analysis, a few major mycotoxins (aflatoxin B1 and G1, fumonisin B1 and B2, and zearalenone) regulated in food crops by the Commission of the European Union were found at concentrations which are toxicologically acceptable in many other crops. Some bioactive compounds were detected at low concentrations in the cassava products. Therefore, the exposure of cassava consumers in Nigeria to regulated mycotoxins was estimated to be minimal. The results provide useful information regarding the probable safety of cassava products in Nigeria.
Biomimetic molecular design tools that learn, evolve, and adapt
Authors: Winkler David A
Published: June 2017

A dominant hallmark of living systems is their ability to adapt to changes in the environment by learning and evolving. Nature does this so superbly that intensive research efforts are now attempting to mimic biological processes. Initially this biomimicry involved developing synthetic methods to generate complex bioactive natural products. Recent work is attempting to understand how molecular machines operate so their principles can be copied, and learning how to employ biomimetic evolution and learning methods to solve complex problems in science, medicine and engineering. Automation, robotics, artificial intelligence, and evolutionary algorithms are now converging to generate what might broadly be called in silico-based adaptive evolution of materials. These methods are being applied to organic chemistry to systematize reactions, create synthesis robots to carry out unit operations, and to devise closed loop flow self-optimizing chemical synthesis systems. Most scientific innovations and technologies pass through the well-known “S curve”, with slow beginning, an almost exponential growth in capability, and a stable applications period. Adaptive, evolving, machine learning-based molecular design and optimization methods are approaching the period of very rapid growth and their impact is already being described as potentially disruptive. This paper describes new developments in biomimetic adaptive, evolving, learning computational molecular design methods and their potential impacts in chemistry, engineering, and medicine.
A Control Theory Model of Smoking
Authors: Bobashev Georgiy , Holloway John , Solano Eric , Gutkin Boris
Published: June 2017

We present a heuristic control theory model that describes smoking under restricted and unrestricted access to cigarettes. The model is based on the allostasis theory and uses a formal representation of a multiscale opponent process. The model simulates smoking behavior of an individual and produces both short-term (“loading up” after not smoking for a while) and long-term smoking patterns (e.g., gradual transition from a few cigarettes to one pack a day). By introducing a formal representation of withdrawal- and craving-like processes, the model produces gradual increases over time in withdrawal- and craving-like signals associated with abstinence and shows that after 3 months of abstinence, craving disappears. The model was programmed as a computer application allowing users to select simulation scenarios. The application links images of brain regions that are activated during the binge/intoxication, withdrawal, or craving with corresponding simulated states. The model was calibrated to represent smoking patterns described in peer-reviewed literature; however, it is generic enough to be adapted to other drugs, including cocaine and opioids. Although the model does not mechanistically describe specific neurobiological processes, it can be useful in prevention and treatment practices as an illustration of drug-using behaviors and expected dynamics of withdrawal and craving during abstinence.
EDITORIAL: SPECIAL ISSUE ON BIOCHAR AS AN OPTION FOR SUSTAINABLE RESOURCE MANAGEMENT (EU COST ACTION TD1107 FINAL PUBLICATION)
Authors: GLASER Bruno , BALTRĖNAS Pranas , KAMMANN Claudia , KERN Jürgen , BALTRĖNAITĖ Edita
Published: June 2017

The articles appearing in this special issue on Biochar as an Option for Sustainable Resource Management are mainly the extended versions of the contributions presented in Biochar COST Action meetings, especially at the International Biocharconference held September 2015 at Geisenheim University (Germany), which was the final conference of the COST Action TD1107.
Photometric Study of Fourteen Low-mass Binaries∗
Authors: Korda D. , Zasche P. , Wolf M. , Kučáková H. , Hoňková K. , Vraštil J.
Published: June 2017

New CCD photometric observations of fourteen short-period low-mass eclipsing binaries (LMBs) in the photometric filters I, R, and V were used for a light curve analysis. A discrepancy remains between observed radii and those derived from the theoretical modeling for LMBs, in general. Mass calibration of all observed LMBs was performed using only the photometric indices. The light curve modeling of these selected systems was completed, yielding the new derived masses and radii for both components. We compared these systems with the compilation of other known double-lined LMB systems with uncertainties of masses and radii less then 5%, which includes 66 components of binaries where both spectroscopy and photometry were combined together. All of our systems are circular short-period binaries, and for some of them, the photospheric spots were also used. A purely photometric study of the light curves without spectroscopy seems unable to achieve high enough precision and accuracy in the masses and radii to act as meaningful test of the M–R relation for low-mass stars.
Evidence of a Spectral Break in the Gamma-Ray Emission of the Disk Component of the Large Magellanic Cloud: A Hadronic Origin?
Authors: Tang Qing-Wen , Peng Fang-Kun , Liu Ruo-Yu , Tam Pak-Hin Thomas , Wang Xiang-Yu
Published: June 2017

It has been suggested that the high-energy gamma-ray emission (

) of nearby star-forming galaxies may be produced predominantly by cosmic rays colliding with the interstellar medium through neutral pion decay. The pion decay mechanism predicts a unique spectral signature in the gamma-ray spectrum, characterized by a fast-rising spectrum (in

representation) and a spectral break below a few hundred MeV. Here, we report evidence of a spectral break, around 500 MeV in the disk emission of the Large Magellanic Cloud (LMC), that was found during an analysis of the gamma-ray data extending down to 60 MeV, observed by the Fermi-Large Area Telescope. The break is consistent with the pion decay model of gamma-ray emission, although leptonic models, such as electron bremsstrahlung emission, cannot be ruled out completely.
Les faux-semblants de l’indépendance
Authors: Morice Alain
Published: June 2017

Bien qu’elles ne soient pas réservées aux étrangers, de nouvelles formes de mise au travail se sont répandues dans les secteurs qui les emploient. Les activités dites « indépendantes » montent en puissance, qui, sous un extérieur libérateur, ont souvent pour effet de surtout libérer l’employeur : rebaptisé « donneur d’ordres (ou d’ouvrage) » ou « client », il peut ainsi s’exonérer de toute obligation contractuelle vis-à-vis du travailleur, qui devient « prestataire ». Cette prolifération est en harmonie avec le retour en force d’un pilier de l’idéologie libérale, l’esprit d’entreprise associé au risque individuel 1 , auquel s’opposerait un salariat canonique réputé porteur d’irresponsabilité.
Sanctions d’employeurs, le renoncement français
Authors: Zissmann Agnès
Published: June 2017

La loi dite « Besson » du 16 juin 2011 transpose en droit français plusieurs directives européennes qui concernent les ressortissants des pays tiers, dont celle de 2009 qui prévoit des sanctions à l’égard des employeurs d’étrangers et d’étrangères en situation irrégulière. Si la loi française intègre bien les sanctions pénales et administratives prévues pour les employeurs, elle oublie tout ce qui concerne les droits sociaux et les indemnités des salarié·e·s sans papiers. Une omission volontaire ?
Définir la valeur d’un humain
Authors: Jakšić Milena , Ragaru Nadège
Published: June 2017

Ces dernières années, on assiste à une prolifération législative autour des victimes d’exploitation sexuelle. La traite des êtres humains a même été érigée au rang de grande cause nationale. Mais au-delà des discours compassionnels, qu’en est-il de l’indemnisation de ces victimes qui, du fait de leur double stigmate de sans-papiers et de prostituées, peinent à accéder à leurs droits et combien coûte le préjudice de la traite ? Enquête auprès des commissions d’indemnisation des victimes d’infractions.
L’Anafé : une lutte au pied des pistes
Authors: Ferré Nathalie , Boutron Pauline
Published: June 2017

Témoins, à la fin des années 1980, des pratiques des services de contrôle aux frontières et du maintien de nombreux étrangers en zone internationale, des salariés des aéroports et des compagnies aériennes, et certaines organisations syndicales se sont rapprochés des associations de défense des droits de l’Homme et des droits des étrangers. De cette collaboration est née l’Association nationale d’assistance aux frontières des étrangers. Patrick Delouvin et Laurent Giovannoni, qui y représentaient respectivement Amnesty International France et la Cimade, et François Julien-Laferrière, alors secrétaire général de France terre d’asile, devenu le premier président de l’Anafé, reviennent sur sa naissance.
Hemophilic Arthropathy
Authors: Nacca Christopher R. , Harris Andrew P. , Tuttle John R.
Published: June 2017

The musculoskeletal manifestations of hemophilia A and B are some of the most common presenting symptoms and continue to be challenging to practitioners. Hemophilic arthropathy, if not initially adequately treated and managed, may lead to debilitating disease and eventually require the consideration of major surgery, including total joint arthroplasty. Thorough comprehension of the pathophysiology, diagnosis, and both medical and surgical interventions is critical in establishing an appropriate treatment regimen for these patients. Furthermore, a true multidisciplinary approach involving hematology, orthopedics, and physical therapy is essential for a patient with hemophilic arthropathy. The authors present a comprehensive review of hemophilic arthropathy from an orthopedist's perspective. [
Orthopedics.
2017; 40(6):e940–e946.]