bluuebunny/crossref_metadata_2025 huggingface dataset:
Single anthocyanins effectiveness modulating inflammation markers in obesity: dosage and matrix composition analysis
Authors: Fragoso-Medina Jorge Alberto , López Vaquera Selma Romina , Domínguez-Uscanga Astrid , Luna-Vital Diego , García Noemí
Published: November 2023

Anthocyanins (ACNs) are phytochemicals with numerous bioactivities, e.g., antioxidant and anti-inflammatory. Health benefits from consuming ACN-rich foods, extracts, and supplements have been studied in clinical trials (CT). However, the individual effect of single ACNs and their correlation with doses and specific bioactivities or molecular targets have not been thoroughly analyzed. This review shows a recompilation of single anthocyanins composition and concentrations used in CT, conducted to investigate the effect of these anti-inflammatory derivatives in obese condition. Single anthocyanin doses with changes in the levels of frequently monitored markers were correlated. In addition, the analysis was complemented with reports of studies made in vitro with single ACNs. Anthocyanins' efficacy in diseases with high baseline obesity-related inflammation markers was evidenced. A poor correlation was found between most single anthocyanin doses and level changes of commonly monitored markers. Correlations between cyanidin, delphinidin, and pelargonidin derivatives and specific molecular targets were proposed. Our analysis showed that knowledge of specific compositions and anthocyanin concentrations determined in future studies would provide more information about mechanisms of action.
Chemical characterization and metabolic profiling of Xiao-Er-An-Shen Decoction by UPLC-QTOF/MS
Authors: Yang Ruipei , Wei Lifang , Wang Jie , Huang Shiying , Mo Pingli , Chen Qiugu , Zheng Ping , Chen Jihang , Zhang Shangbin , Chen Jianping
Published: November 2023

Background: Xiao-Er-An-Shen decoction (XEASD), a TCM formula composed of sixteen Chinese medicinal herbs, has been used to alleviate tic disorders (TD) in clinical practice for many years. However, the chemical basis underlying the therapeutic effects of XEASD in the treatment of TD remains unknown.

Purpose: The present study aimed to determine the major chemical components of XEASD and its prototype compounds and metabolites in mice biological samples.

Methods: The chemical constituents in XEASD were identified using ultra-high Performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). Following this, XEASD was orally administered to mice, and samples of plasma, urine, feces, bile, and tissue were collected in order to identify effective compounds for the prevention or treatment of TD.

Result: Of the total 184 compounds identified to be discriminated in the XEASD, comprising 44 flavonoids, 26 phenylpropanoids, 16 coumarins, 16 triterpenoids, 14 amino acids, 13 organic acids, 13 alkaloids, 13 ketones, 10 cyclic enol ether terpenes, 7 citrullines, 3 steroids, and 5 anthraquinones, and others. Furthermore, we summarized 54 prototype components and 78 metabolic products of XEASD, measured with biological samples, by estimating metabolic principal components, with four prototype compounds detected in plasma, 58 prototypes discriminated in urine, and 40 prototypes identified in feces. These results indicate that the Oroxylin A glucuronide from Citri reticulatae pericarpium (CRP) is a major compound with potential therapeutic effects identified in brain, while operating positive effect in inhibiting oxidative stress in vitro.

Conclusion: In summary, our work delineates the chemical basis underlying the complexity of XEASD, providing insights into the therapeutic and metabolic pathways for TD. Various types of chemicals were explored in XEASD, including flavonoids, phenylpropanoids, coumarins, organic acids, triterpenoid saponins, and so on. This study can promote the further pharmacokinetic and pharmacological evaluation of XEASD.
Agronomic strategies to enhance the early vigor and yield of maize. Part I: the role of seed applied biostimulant, hybrid and starter fertilization on rhizosphere bacteria profile and diversity
Authors: Ujvári Gergely , Capo Luca , Grassi Arianna , Cristani Caterina , Pagliarani Irene , Turrini Alessandra , Blandino Massimo , Giovannetti Manuela , Agnolucci Monica
Published: November 2023

The sustainable intensification of maize-based systems may reduce greenhouse-gas emissions and the excessive use of non-renewable inputs. Considering the key role that the microbiological fertility has on crop growth and resilience, it is worth of interest studying the role of cropping system on the rhizosphere bacterial communities, that affect soil health and biological soil fertility. In this work we monitored and characterized the diversity and composition of native rhizosphere bacterial communities during the early growth phases of two maize genotypes of different early vigor, using a nitrogen (N)-phosphorus (P) starter fertilization and a biostimulant seed treatment, in a growth chamber experiment, by polymerase chain reaction-denaturing gradient gel electrophoresis of partial 16S rRNA gene and amplicon sequencing. Cluster analyses showed that the biostimulant treatment affected the rhizosphere bacterial microbiota of the ordinary hybrid more than that of the early vigor, both at plant emergence and at the 5-leaf stage. Moreover, the diversity indices calculated from the community profiles, revealed significant effects of NP fertilization on richness and the estimated effective number of species (H2) in both maize genotypes, while the biostimulant had a positive effect on plant growth promoting community of the ordinary hybrid, both at the plant emergence and at the fifth leaf stage. Our data showed that maize genotype was the major factor shaping rhizosphere bacterial community composition suggesting that the root system of the two maize hybrids recruited a different microbiota. Moreover, for the first time, we identified at the species and genus level the predominant native bacteria associated with two maize hybrids differing for vigor. These results pave the way for further studies to be performed on the effects of cropping system and specific crop practices, considering also the application of biostimulants, on beneficial rhizosphere microorganisms.
Navon letters and composite faces: same or different processing mechanisms?
Authors: Fitousi Daniel , Azizi Omer
Published: November 2023

Navon letters and composite faces are two fascinating demonstrations of hierarchical organization in perception. Many researchers believe that the two types of stimuli and their associated tasks gauge comparable holistic mechanisms. This belief is so common that the two paradigms are now being applied in tandem to measure impaired holistic processing in prosopagnosic patients. But are Navon letters and composite faces processed in a similar fashion? In the present study we take a closer look at their apparent affinity. We gain novel insights into their underlying mechanisms by fitting parameters of the linear ballistic accumulator (LBA) model to empirical correct and incorrect response times (RTs). The results reveal major differences in processing between the two tasks. We conclude that despite the presence of a compelling surface similarity, Navon compound letters and composite faces tap into separate psychological processes.
Associations between teacher-student relationship and externalizing problem behaviors among Chinese rural adolescent
Authors: Yang Shuping , Zhu Xingchen , Li Wencan , Zhao Haohan
Published: November 2023

The primary objective of this study is to present a fresh perspective on the correlation between teacher-student relationships and externalizing problem behaviors among adolescents. While previous research has examined this connection, there is still an insufficient understanding of the underlying mechanisms. Moreover, the crucial role of peer relationships, mental health, and parental knowledge has been overlooked. In this study, a total of 6,919 Chinese rural adolescents aged 13–19 years participated by completing an anonymous self-report questionnaire. The results show that: (1) teacher-student relationship has a protective effect against the development of externalizing problem behaviors; (2) peer relationship and mental health both have a mediating role in the relationship between teacher-student relationship and externalizing problem behaviors; (3) teacher-student relationship can indirectly affect externalizing problem behaviors through the chain mediation of peer relationship and mental health; (4) parental knowledge plays a moderating role between the teacher-student relationship and externalizing problem behaviors. As the level of parental knowledge increases among rural adolescents, the impact of the teacher-student relationship on externalizing problem behaviors becomes more pronounced; and (5) the impact of teacher-student relationship on externalizing problem behaviors has no significant gender differences. Given the study’s empirical outcomes, we discuss potential explanations and advocate for a comprehensive pedagogical approach to mitigate rural adolescent externalizing behaviors. This entails nurturing teacher-student relations, fostering inclusive peer environments, emphasizing mental health literacy, and synergizing with caregivers for a holistic home-school intervention.
Does obesity Kuznets curve exist in developing economies? Evidence from 38 African countries based on heterogeneous panel data analysis on income-level classification
Authors: Chen Hao , Atingabili Samuel , Mensah Isaac Adjei , Yaw Omari-Sasu Akoto , Agba Tackie Evelyn , Arboh Francisca , Danso Bertha Ada
Published: November 2023

Introduction

The global pandemic disease known as the obesity epidemic has spread throughout the planet. Particularly, Africa is facing a growing problem of obesity, and the trend is rising. This is a result of a ticking time bomb. Given the claim that multiple socio-economic factors significantly affect the diversity in obesity rates between nations, economic development can be seen as a key contributor to this variation.

Methods

Relying on the aforementioned avowal, this extant research examines the relationship between obesity and economic growth using urbanization, trade openness, and unemployment as intermittent variables within the Obesity Kuznets Curve (OKC) framework. Using panel data from 1990 to 2020, a panel of 38 African countries subdivided into income levels (Low income, Lower-middle income, and Upper-middle income) were analyzed. With the presence of residual cross-sectional reliance and slope heterogeneity, the Augmented Mean Group (AMG) econometric approach is employed.

Results

Key outcomes from the mentioned estimation method unveiled that economic growth positively impacts obesity among all the study panels. Variably, unemployment was evidenced to have a palpable positive impact on obesity concerning Low-income economies whereas on the side of the Lower-middle income panel together with Upper-middle income economies and the aggregated panel, a significant negative relationship is observed with obesity. Further, urbanization enhanced obesity in the Low-income panel and the aggregated panel of African nations, whereas an adverse effect is identified in both the Lower-middle and Upper-middle economies in Africa. Moreover, except for Low-income African economies, all the other panels of African nations in terms of income levels were noted to have a significant negative effect on obesity from trade openness.

Discussion

Finally, the long-run coefficients indicated that the OKC is valid among all panels of African countries. The study thus preferably suggests in African economies that addressing the inverted U-shape relationship between obesity and economic growth requires a multifaceted approach that considers the evolving dynamics of both factors. Policy makers should, therefore, aim to balance promoting economic growth and safeguarding public health through targeted interventions and long-term strategies.
Evaluation of the efficacy of the Lentulo spiral filler operated at four different speeds and with two different techniques in cadaveric canine teeth of dogs
Authors: Carlson Haley , Montebello Jennifer , Lee Bonnie , Rendahl Aaron , Goldschmidt Stephanie
Published: November 2023

This study evaluated the effect of filling technique and rotational speed on the efficacy of sealer cement placement using the Lentulo spiral filler. Cadaveric maxillary and mandibular canine teeth (n = 74) from age and breed matched fresh-frozen adult canine cadaver heads were utilized. Following routine mechano-chemical preparation, teeth were randomly divided into 8 treatment groups with varying rotational speeds [250–2,000 revolutions per minute (RPM)] and fill techniques (backfill technique versus pumping technique). The quality of the sealer cement fill was evaluated radiographically before and after master gutta percha cone placement. Percentage of voids present in the apical, midbody, and coronal portions of the tooth were subjectively scored as: no voids present, voids present in less than 25% of the area, voids present in 25–50% of the area, voids present in greater than 50% of the area. The apex was also scored as underfilled, adequately filled, or overfilled. Operating the Lentulo spiral filler at 2,000 RPM resulted in the best quality fill regardless of filling technique. The pumping technique with placement of the master gutta percha cone had a higher probability of success as compared to the backfill technique, but this finding lacked significance. Regardless of speed or technique, the apical region had the highest quality of fill. The addition of the master gutta percha cone improved the quality of fill. Therefore, we recommend using the Lentulo spiral filler at higher speeds with a pumping technique (followed by addition of a master gutta percha cone) to improve the quality of epoxy resin-based sealer cement placement.
Effects of Jujube Powder on Growth Performance, Blood Biochemical Indices, and Intestinal Microbiota of Broiler
Authors: Liang Jing , Nie Zejian , Zhao Yapeng , Qin Shizhen , Nian Fang , Tang Defu
Published: November 2023

In total, 576 Cobb broilers were randomized into 6 treatment groups, with 8 replicates in each treatment group and 12 broilers in each replicate. Each treatment group was fed six different experimental diets containing 0%, 2%, 4%, 6%, 8%, and 10% jujube powder. The group receiving 0% jujube powder was considered the blank control group. The experimental period was 42 days and was divided into two periods: starter (0–21 days) and finisher (22–42 days). Compared with the control group, the addition of 8% jujube powder significantly improved the ADG of broilers (p < 0.05), and 8% and 10% jujube powder significantly improved the total tract apparent digestibility of organic matter in broilers (p < 0.05). Adding 10% jujube powder significantly improved the apparent metabolic energy of broilers (p < 0.05). Compared with the control group, 4–10% jujube powder significantly increased IgA, IgG, IgM, and sCD4 levels (p < 0.05) and T-AOC and SOD contents, and it reduced the MDA content in the serum of broilers (p < 0.05). In addition, the relative abundance of Firmicutes, Bacteroidetes, Lactobacillus, and Romboutsia significantly increased in the broiler ileum, whereas that of Proteobacteria and Enterobacter decreased significantly (p < 0.05) when 8% jujube powder was added to the diet. The relative abundance of Proteobacteria, Bacteroides, and Faecalibacterium in the cecum increased significantly (p < 0.05), whereas that of Bacteroidetes decreased significantly (p < 0.05).
Definition of a Global Coordinate System in the Foot for the Surgical Planning of Forefoot Corrections
Authors: Krakers Sanne , Peters Anil , Homan Sybrand , olde Heuvel Judith , Tuijthof Gabriëlle
Published: November 2023

Forefoot osteotomies to improve the alignment are difficult procedures and can lead to a variety of complications. Preoperative planning in three dimensions might assist in the successful management of forefoot deformities. The purpose of this study was to develop a global coordinate system in the foot for the planning of forefoot corrections. Two strategies (CS1 and CS2) were developed for defining a global coordinate system that meets the criteria of being well-defined, robust, highly repeatable, clinically relevant, compatible with foot CT scans, independent of the ankle joint angle, and does not include bones in the forefoot. The absolute angle of rotation was used to quantify repeatability. The anatomical planes of the coordinate systems were visually inspected by an orthopedic surgeon to evaluate the clinical relevancy. The repeatability of CS1 ranged from 0.48° to 5.86°. The definition of CS2 was fully automated and, therefore, had a perfect repeatability (0°). Clinically relevant anatomical planes were observed with CS2. In conclusion, this study presents an automated method for defining a global coordinate system in the foot according to predefined requirements for the planning of forefoot corrections.
Surgical Clipping of Intracranial Aneurysms Using a Transcranial Neuroendoscopic Approach
Authors: Li Zhiyang , Lei Pan , Hua Qiuwei , Zhou Long , Song Ping , Gao Lun , Zhang Silei , Cai Qiang
Published: November 2023

Objective: This retrospective study was performed to evaluate the feasibility and safety of surgically clipping intracranial aneurysms using a transcranial neuroendoscopic approach. Methods: A total of 229 patients with cerebral aneurysms were included in our study, all of whom were treated with clamping surgery at Wuhan University People’s Hospital. They were divided into neuroendoscopic and microscopic groups, according to whether or not neuroendoscopy was used for the clamping surgery. We statistically analyzed the patients’ baseline data, surgical outcomes, and complications, which were then evaluated to assess the treatment effect. Results: The baseline characteristics were not statistically significant, except for gender, for which the proportions of female patients in the two groups were 69 (56.1%) and 46 (43.4%). There were no patients with incomplete aneurysm clamping or parent vessel occlusion in the neuroendoscopic group, and there were 4 (3.8%) and 2 (1.9%) in the microscopic group, respectively; however, there was no statistically significant difference in the comparison of the two groups. The mean operative times of the two groups were 181 min and 154 min, respectively, and were statistically different. However, the mRS scores of the two groups showed no significant difference in patient prognosis. The differences in complications (including limb hemiplegia, hydrocephalus, vision loss, and intracranial infection) were not statistically significant, except for cerebral ischemia, for which the proportions of patients in the two groups were 8 (6.5%) and 16 (15.1%). Conclusions: Neuroendoscopy can provide clear visualization and multi-angle views during aneurysm clipping, which is helpful for ensuring adequate clipping and preventing complications.
Walk Locomotion Kinematic Changes in a Model of Penetrating Hippocampal Injury in Male/Female Mice and Rats
Authors: Alpirez Jonatan , Leon-Moreno Lilia Carolina , Aguilar-García Irene Guadalupe , Castañeda-Arellano Rolando , Dueñas-Jiménez Judith Marcela , Asencio-Piña Cesar Rodolfo , Dueñas-Jiménez Sergio Horacio
Published: November 2023

Traumatic brain injury has been the leading cause of mortality and morbidity in human beings. One of the most susceptible structures to this damage is the hippocampus due to cellular and synaptic loss and impaired hippocampal connectivity to the brain, brain stem, and spinal cord. Thus, hippocampal damage in rodents using a stereotaxic device could be an adequate method to study a precise lesion from CA1 to the dentate gyrus structures. We studied male and female rats and mice, analyzing hindlimb locomotion kinematics changes to compare the locomotion kinematics using the same methodology in rodents. We measure (1) the vertical hindlimb metatarsus, ankle, and knee joint vertical displacements (VD) and (2) the factor of dissimilarity (DF). The VD in intact rats in metatarsus, ankle, and knee joints differs from that in intact mice in similar joints. In rats, the vertical displacement through the step cycle changed in the left and right metatarsus, ankle, and knee joints compared to the intact group versus the lesioned group. More subtle changes were also observed in mice. DF demonstrates contrasting results when studying locomotion kinematics of mice or rats and sex-dependent differences. Thus, a precise lesion in a rodent’s hippocampal structure discloses some hindlimb locomotion changes related to species and sex. Thus, we only have a qualitative comparison between murine species. In order to make a comparison with other species, we should standardize the model.
Impact of Human Papillomavirus-Negative Dominance in Oropharyngeal Cancer on Overall Survival: A Population-Based Analysis in Germany from 2018 to 2020
Authors: Kouka Mussab , Gerlach Laura , Büntzel Jens , Kaftan Holger , Böger Daniel , Müller Andreas H. , Ernst Thomas , Guntinas-Lichius Orlando
Published: November 2023

The impact of the relation of human papillomavirus (HPV) and smoking status of oropharyngeal squamous cell carcinoma (OPSCC) on overall survival (OS) was investigated in a retrospective population-based study in Thuringia, Germany. A total of 498 patients with OPSCC (76.9% men; mean age 62.5 years) from 2018 to 2020 were included. OPSCC cases were 37.3% HPV-positive (+) (31.2% smokers; mean incidence: 2.91/100,000 population) and 57.8% HPV-negative (63.5% smokers; mean incidence: 4.50/100,000 population). Median follow-up was 20 months. HPV+ patients had significantly better OS than HPV-negative (−) patients (HPV+: 2-year OS: 90.9%; HPV−: 2-year OS: 73.6%; p < 0.001). In multivariable analysis, HPV− patients (hazard ratio (HR) = 4.5; 95% confidence interval (CI): 2.4–8.6), patients with higher N classification (N2: HR = 3.3; 95% CI: 1.71–6.20; N3: HR = 3.6; 95% CI: 1.75–7.31) and with a higher cancer staging (III: HR = 5.7; 95% CI: 1.8–17.6; IV: HR = 19.3; 95% CI: 6.3–57.3) had an increased hazard of death. HPV− smokers formed the majority in Thuringia. Nicotine and alcohol habits had no impact on OS. Optimizing OPSCC therapeutic strategies due to the dominance of HPV− is more important than discussing de-escalation strategies for HPV+ patients.
Treating Parkinson’s Disease with Human Bone Marrow Mesenchymal Stem Cell Secretome: A Translational Investigation Using Human Brain Organoids and Different Routes of In Vivo Administration
Authors: Mendes-Pinheiro Bárbara , Campos Jonas , Marote Ana , Soares-Cunha Carina , Nickels Sarah L. , Monzel Anna S. , Cibrão Jorge R. , Loureiro-Campos Eduardo , Serra Sofia C. , Barata-Antunes Sandra , Duarte-Silva Sara , Pinto Luísa , Schwamborn Jens C. , Salgado António J.
Published: November 2023

Parkinson’s disease (PD) is the most common movement disorder, characterized by the progressive loss of dopaminergic neurons from the nigrostriatal system. Currently, there is no treatment that retards disease progression or reverses damage prior to the time of clinical diagnosis. Mesenchymal stem cells (MSCs) are one of the most extensively studied cell sources for regenerative medicine applications, particularly due to the release of soluble factors and vesicles, known as secretome. The main goal of this work was to address the therapeutic potential of the secretome collected from bone-marrow-derived MSCs (BM-MSCs) using different models of the disease. Firstly, we took advantage of an optimized human midbrain-specific organoid system to model PD in vitro using a neurotoxin-induced model through 6-hydroxydopamine (6-OHDA) exposure. In vivo, we evaluated the effects of BM-MSC secretome comparing two different routes of secretome administration: intracerebral injections (a two-site single administration) against multiple systemic administration. The secretome of BM-MSCs was able to protect from dopaminergic neuronal loss, these effects being more evident in vivo. The BM-MSC secretome led to motor function recovery and dopaminergic loss protection; however, multiple systemic administrations resulted in larger therapeutic effects, making this result extremely relevant for potential future clinical applications.
Study on Green Controllable Preparation of Coal Gangue-Based 13-X Molecular Sieves and Its CO2 Capture Application
Authors: Yi Dawei , Du Huiling , Li Yefei , Gao Yimin , Liu Sifan , Xu Boyang , Huang Haoqi , Kang Le
Published: November 2023

Carbon dioxide emissions are the primary and most direct contributor to global warming, posing a significant hazard to both the environment and human health. In response to this challenge, there has been a growing interest in the development of effective carbon capture technologies. This study involved the synthesis of 13-X molecular sieve porous materials using solid waste coal gangue as a source of silicon and aluminum. The synthesis process involved the controlled utilization of an “alkali fusion-hydrothermal” reaction system. The resulting materials were characterized for their structure, morphology, and crystal composition using X-ray diffraction and field emission scanning electron microscopy. These 13-X molecular sieve materials were employed as adsorbents to capture carbon dioxide gas, and their adsorption performance was investigated. The findings indicated that the 13-X molecular sieve materials possess uniform pores and complete crystalline morphologies, and they exhibited an adsorption capacity of 1.82 mmol/g for carbon dioxide at 0 °C. Consequently, this study not only converted solid waste gangue into high-value products but also demonstrated effective atmospheric carbon dioxide capture, suggesting that gangue-based 13-X molecular sieves may serve as a potential candidate for carbon capture.
Transport Properties of Equiatomic CoCrFeNi High-Entropy Alloy with a Single-Phase Face-Centered Cubic Structure
Authors: Bykov Victor A. , Kulikova Tatyana V. , Sipatov Ivan S. , Sterkhov Eugene V. , Kovalenko Darya A. , Ryltsev Roman E.
Published: November 2023

The key thermophysical properties necessary for the successful design and use of CoCrFeNi alloy in thermophysical applications have been measured experimentally, and the results have been compared with literature values and results previously obtained for commercial Ni-Cr alloys and equiatomic CoCrFeNi alloy. In particular, the thermal diffusivity, coefficient of thermal expansion (CTE), and specific heat capacity were measured for the as-cast and homogenized equiatomic CoCrFeNi alloy over a temperature range allowing the thermal conductivity to be calculated up to 1173 K. The thermal conductivity and thermal diffusivity of the equiatomic CoCrFeNi alloy were found to deviate from monotonic behavior in the temperature range from 773 to 1100 K. Such a deviation was previously observed in the behavior of the temperature dependence of CTE and specific heat capacity of the equiatomic CoCrFeNi alloy. The non-linear behavior is primarily the result of order/disorder phenomena for the as-cast and homogenized sample, as well as non-equilibrium solidification under arc melting conditions for the as-cast sample. The measured data of thermophysical properties are provided for thermally differently treated samples, and it is shown that there is a difference in the behavior of the temperature dependences of CTE, thermal diffusivity, and heat capacity.
Estimating Field-Level Perennial Bioenergy Grass Biomass Yields Using the Normalized Difference Red-Edge Index and Linear Regression Analysis for Central Virginia, USA
Authors: Hamada Yuki , Zumpf Colleen R. , Quinn John J. , Negri Maria Cristina
Published: November 2023

We investigated the indicative power of the normalized difference red-edge index (NDRE) for estimating field-level perennial bioenergy grass biomass yields utilizing Sentinel-2 imagery and a linear regression model as a rapid, cost-effective method for biomass yield estimations for bioenergy. We used 2019 data from three study sites containing mature perennial bioenergy grass stands in central Virginia, USA. Of the simulated daily NDRE values based on the temporally weighted averaging of two temporal neighbors, we found the strongest index–yield correlation on 11 August (R = 0.85). We estimated the perennial bioenergy grass biomass yields for (1) all sites using the data pooled from the three sites (all-site estimation) and (2) each site using the data pooled from the other two sites (cross-site estimation). The estimated field-level perennial bioenergy grass biomass yields strongly correlated with the recorded yields (average R2 = 0.76), with a root mean square error (RMSE) of 1.5 Mg/ha and a mean absolute error (MAE) of 1.2 Mg/ha for the all-site estimation. For the cross-site estimation, the site with diverse perennial grass types had the weakest correlation (R2 = 0.44) of the sites, indicating a difficulty in accounting for heterogeneous index–yield relationships in a single model. In addition to identifying a strong indicative power of the NDRE for estimating the overall perennial bioenergy grass biomass yields at a field level, the findings from this study call for an analysis across multiple perennial grasses and a comparison using multiple sites to understand (1) if the indicative power of the index shifts from the biomass of the specific perennial bioenergy grass type to the overall biomass during the growing season and (2) the level of perennial bioenergy grass heterogeneity that may hinder the remotely sensed biomass yield estimation using a single model.
An Agricultural Biogas Plant as a Thermodynamic System: A Study of Efficiency in the Transformation from Primary to Secondary Energy
Authors: Pilarski Krzysztof , Pilarska Agnieszka A. , Kolasa-Więcek Alicja , Suszanowicz Dariusz
Published: November 2023

Using a wide range of organic substrates in the methane fermentation process enables efficient biogas production. Nonetheless, in many cases, the efficiency of electricity generation in biogas plant cogeneration systems is much lower than expected, close to the calorific value of the applied feedstock. This paper analyses the energy conversion efficiency in a 1 MWel agricultural biogas plant fed with corn silage or vegetable waste and pig slurry as a feedstock dilution agent, depending on the season and availability. Biomass conversion studies were carried out for 12 months, during which substrate samples were taken once a month. The total primary energy in the substrates was estimated in laboratory conditions by measuring the released heat (17,760 MWh·year−1), and, in the case of pig slurry, biochemical methane potential (BMP, (201.88 ± 3.21 m3·Mg VS−1). Further, the substrates were analysed in terms of their chemical composition, from protein, sugar and fat content to mineral matter determination, among other things. The results obtained during the study were averaged. Based on such things as the volume of the biogas, the amount of chemical (secondary) energy contained in methane as a product of biomass conversion (10,633 MWh·year−1) was calculated. Considering the results obtained from the analyses, as well as the calculated values of the relevant parameters, the biomass conversion efficiency was determined as the ratio of the chemical energy in methane to the (primary) energy in the substrates, which was 59.87%, as well as the electricity production efficiency, as the ratio of the electricity produced (4913 MWh·year−1) to the primary energy, with a 35% cogeneration system efficiency. The full energy conversion efficiency, related to electricity production, reached a low value of 27.66%. This article provides an insightful, unique analysis of energy conversion in an active biogas plant as an open thermodynamic system.
Experimental Investigation of the Dynamic Response of a Flat Blade with Dual Dry Friction Dampers
Authors: Man Jixin , Bian Xiangde , Zeng Wu , Yan Wengao , Qiao Da
Published: November 2023

One test rig comprising two blades and dual under-platform dampers (UPDs) was built to enhance the understanding of the dynamic response behavior of blades with dual UPDs. A turnbuckle was applied to enable the smooth and uninterrupted linear adjustment of the normal load on the dual UPDs. Non-contact vibration-response measurements were achieved through eddy-current displacement sensors. Contact excitation was employed using an electromagnetic exciter to determine the magnitude of the excitation load, which was measured using a force sensor mounted on the excitation rod. A feedback system was established to maintain a constant magnitude of the excitation force throughout the excitation process. The chosen experimental variables include the normal load, the amplitude of the excitation force, the effective contact area, and the position of the damper action. The frequency response function of the blade under various experimental parameters was obtained through frequency sweeping under sinusoidal excitation. The influence of each parameter on the dynamic characteristics of blades was studied. The results demonstrate that the double-layer damping system offers distinct advantages over its single-layer counterpart. The upper damping has a wider frequency-adjustment range and a lower resonance amplitude and takes a larger share of the damping efficiency.
The First Dinosaur from the Kingdom of Cambodia: A Sauropod Fibula from the Lower Cretaceous of Koh Kong Province, South-Western Cambodia
Authors: Lim Vanchan , Buffetaut Eric , Tong Haiyan , Cavin Lionel , Pann Kimchhay , Ngoeun Phalline
Published: November 2023

The first discovery of a dinosaur bone from the Kingdom of Cambodia is reported in this paper. It consists of a sauropod fibula from a sandstone layer on Koh Paur island, in Koh Kong province, in south-western Cambodia. The dinosaur-bearing bed belongs to the non-marine Grès Supérieurs series and is apparently of Early Cretaceous age. On the basis of various characters, notably the development of the anteromedial crest, the dinosaur fibula from Koh Paur is referred to a euhelopodid titanosauriform. This first dinosaur discovery in Cambodia suggests that the thick non-marine formations which cover vast areas in the south-western part of the country are potentially an important source of continental Mesozoic vertebrates.
Magnetic and Magnetostrictive Properties of Sol–Gel-Synthesized Chromium-Substituted Cobalt Ferrite
Authors: Beera Chandra Sekhar , Dhanalakshmi B. , Devi D. Nirmala , Vijayalakshmi D. , Mishra Akanksha , Ramesh S. , Rao B. Parvatheeswara , Shyamala P. , Menelaou Melita , Alanazi Nadyah , Alodhayb Abdullah N.
Published: November 2023

Chromium (Cr)-doped cobalt ferrite nanoparticles were synthesized using a sol–gel autocombustion method, with the chemical formula CoCrxFe2xO4. The value of x ranged from 0.00 to 0.5 in 0.1 increments. X-ray diffraction analysis confirmed the development of highly crystalline cubic spinel structures for all samples, with an average crystallite size of approximately 40 to 45 nm determined using the Scherrer equation. Pellets were prepared using a traditional ceramic method. The magnetic and magnetostrictive properties of the samples were tested using strain gauge and VSM (vibrating sample magnetometer) techniques. The results of the magnetic and magnetostrictive tests showed that the chromium-substituted cobalt ferrites exhibited higher strain derivative magnitudes than pure cobalt ferrite. These findings indicated that the introduction of chromium into the cobalt ferrite structure led to changes in the material’s magnetic properties. These changes were attributed to anisotropic contributions, resulting from an increased presence of Co2+ ions at B-sites due to the chromium substitutions. In summary, this study concluded that introducing chromium into the cobalt ferrite structure caused alterations in the material’s magnetic properties, which were explained by changes in the cationic arrangement within the crystal lattice. This study successfully explained these alterations using magnetization and coercivity data and the probable cationic dispersion.
Perspectives on Advanced Technologies in Spatial Data Collection and Analysis
Authors: Hochmair Hartwig H. , Navratil Gerhard , Huang Haosheng
Published: November 2023

The motivation to organize this Special Issue originated from the observation of rapid changes taking place in the domain of geographical information science and systems over the past few decades [...]
Label-Free Quantitative Proteomics Reveal the Mechanisms of Young Wheat (Triticum aestivum L.) Ears’ Response to Spring Freezing
Authors: Wang Weiling , Zhang Yuting , Liu Chang , Dong Yongwen , Jiang Xue , Zhao Can , Li Guohui , Xu Ke , Huo Zhongyang
Published: November 2023

Late spring frost is an important meteorological factor threatening the safe production of winter wheat in China. The young ear is the most vulnerable organ of the wheat plant to spring frost. To gain an insight into the mechanisms underpinning young wheat ears’ tolerance to freezing, we performed a comparative proteome analysis of wheat varieties Xumai33 (XM33, freezing-sensitive) and Jimai22 (JM22, freezing-tolerant) under normal and freezing conditions using label-free quantitative proteomic techniques during the anther connective tissue formation phase (ACFP). Under freezing stress, 392 and 103 differently expressed proteins (DEPs) were identified in the young ears of XM33 and JM22, respectively, and among these, 30 proteins were common in both varieties. A functional characterization analysis revealed that these DEPs were associated with antioxidant capacity, cell wall modification, protein folding, dehydration response, and plant–pathogen interactions. The young ears of JM22 showed significantly higher expression levels of antioxidant enzymes, heat shock proteins, and dehydrin under normal conditions compared to those of XM33, which might help to prepare the young ears of JM22 for freezing stress. Our results lead to new insights into understanding the mechanisms in young wheat ears’ response to freezing stress and provide pivotal potential candidate proteins required for improving young wheat ears’ tolerance to spring frost.
Exploring the Binding Effects of Natural Products and Antihypertensive Drugs on SARS-CoV-2: An In Silico Investigation of Main Protease and Spike Protein
Authors: Moschovou Kalliopi , Antoniou Maria , Chontzopoulou Eleni , Papavasileiou Konstantinos D. , Melagraki Georgia , Afantitis Antreas , Mavromoustakos Thomas
Published: November 2023

In this in silico study, we conducted an in-depth exploration of the potential of natural products and antihypertensive molecules that could serve as inhibitors targeting the key proteins of the SARS-CoV-2 virus: the main protease (Mpro) and the spike (S) protein. By utilizing Induced Fit Docking (IFD), we assessed the binding affinities of the molecules under study to these crucial viral components. To further comprehend the stability and molecular interactions of the “protein-ligand” complexes that derived from docking studies, we performed molecular dynamics (MD) simulations, shedding light on the molecular basis of potential drug candidates for COVID-19 treatment. Moreover, we employed Molecular Mechanics Generalized Born Surface Area (MM-GBSA) calculations on all “protein-ligand” complexes, underscoring the robust binding capabilities of rosmarinic acid, curcumin, and quercetin against Mpro, and salvianolic acid b, rosmarinic acid, and quercetin toward the S protein. Furthermore, in order to expand our search for potent inhibitors, we conducted a structure similarity analysis, using the Enalos Suite, based on the molecules that indicated the most favored results in the in silico studies. The Enalos Suite generated 115 structurally similar compounds to salvianolic acid, rosmarinic acid, and quercetin. These compounds underwent IFD calculations, leading to the identification of two salvianolic acid analogues that exhibited strong binding to all the examined binding sites in both proteins, showcasing their potential as multi-target inhibitors. These findings introduce exciting possibilities for the development of novel therapeutic agents aiming to effectively disrupt the SARS-CoV-2 virus lifecycle.
Purification, Structural Characterization, and Antitumor Activity of a Polysaccharide from Perilla Seeds
Authors: Li Hui , Liu Ming , Liu Zikun , Cheng Li , Li Mengsha , Li Chongwei
Published: November 2023

A previous study found that a crude Perilla seed polysaccharide (PFSP) fraction exhibited obviously antitumor activity; however, the structural characterization and antitumor properties of this polysaccharide remain unclear. In this study, the PFSP was extracted and purified via combined column chromatography, and the structure of a single polysaccharide fraction was characterized by methylation, IC, GC-MS, NMR, and AFM. The results demonstrated that the efficient antitumor polysaccharide fraction PFSP-2-1 was screened from PFSP with a relative molecular weight of 8.81 × 106 Da. The primary structure of the PFSP main chain was →1)-Araf-(5→, →1,3)-Galp-(6→, →1)-Galp-(6→, →1,3)-Araf-(5→ and →1)-Xylp-(4→, and that of the side chains was →1)-Arap, →1,3)-Galp-(6→, →1)-Araf and →1)-Glcp-(4→, →1)-Galp-(3→ and →1)-Glcp, leading to a three-dimensional helical structure. CCK-8 experiments revealed that PFSP-2-1 significantly inhibited the growth of human hepatocellular carcinoma cells in vitro (p < 0.05), and its inhibitory effect positively correlation with the concentration of PFSP-2-1, and when the concentration of PFSP-2-1 was 1600 µg/mL, it showed the highest inhabitation rate on three hepatocellular carcinoma cells (HepG-2, Hep3b, and SK-Hep-1), for which the survival rates of HepG-2, Hep3b, and SK-Hep-1 were 53.34%, 70.33%, and 71.06%. This study clearly elucidated the structure and antitumor activity of PFSP-2-1, which lays a theoretical foundation for revealing the molecular mechanism of antitumor activity of Perilla seed polysaccharides and provides an important theoretical basis for the development of high-value Perilla.
Therapeutic Strategies for Pancreatic-Cancer-Related Type 2 Diabetes Centered around Natural Products
Authors: Park Moon Nyeo
Published: November 2023

Pancreatic ductal adenocarcinoma (PDAC), a highly malignant neoplasm, is classified as one of the most severe and devastating types of cancer. PDAC is a notable malignancy that exhibits a discouraging prognosis and a rising occurrence. The interplay between diabetes and pancreatic cancer exhibits a reciprocal causation. The identified metabolic disorder has been observed to possess noteworthy consequences on health outcomes, resulting in elevated rates of morbidity. The principal mechanisms involve the suppression of the immune system, the activation of pancreatic stellate cells (PSCs), and the onset of systemic metabolic disease caused by dysfunction of the islets. From this point forward, it is important to recognize that pancreatic-cancer-related diabetes (PCRD) has the ability to increase the likelihood of developing pancreatic cancer. This highlights the complex relationship that exists between these two physiological states. Therefore, we investigated into the complex domain of PSCs, elucidating their intricate signaling pathways and the profound influence of chemokines on their behavior and final outcome. In order to surmount the obstacle of drug resistance and eliminate PDAC, researchers have undertaken extensive efforts to explore and cultivate novel natural compounds of the next generation. Additional investigation is necessary in order to comprehensively comprehend the effect of PCRD-mediated apoptosis on the progression and onset of PDAC through the utilization of natural compounds. This study aims to examine the potential anticancer properties of natural compounds in individuals with diabetes who are undergoing chemotherapy, targeted therapy, or immunotherapy. It is anticipated that these compounds will exhibit increased potency and possess enhanced pharmacological benefits. According to our research findings, it is indicated that naturally derived chemical compounds hold potential in the development of PDAC therapies that are both safe and efficacious.
Independent and Interactive Roles of Immunity and Metabolism in Aortic Dissection
Authors: Li Siyu , Li Jun , Cheng Wei , He Wenhui , Dai Shuang-Shuang
Published: November 2023

Aortic dissection (AD) is a cardiovascular disease that seriously endangers the lives of patients. The mortality rate of this disease is high, and the incidence is increasing annually, but the pathogenesis of AD is complicated. In recent years, an increasing number of studies have shown that immune cell infiltration in the media and adventitia of the aorta is a novel hallmark of AD. These cells contribute to changes in the immune microenvironment, which can affect their own metabolism and that of parenchymal cells in the aortic wall, which are essential factors that induce degeneration and remodeling of the vascular wall and play important roles in the formation and development of AD. Accordingly, this review focuses on the independent and interactive roles of immunity and metabolism in AD to provide further insights into the pathogenesis, novel ideas for diagnosis and new strategies for treatment or early prevention of AD.
The Biological Assessment of Shikonin and β,β-dimethylacrylshikonin Using a Cellular Myxofibrosarcoma Tumor Heterogeneity Model
Authors: Lohberger Birgit , Kaltenegger Heike , Eck Nicole , Glänzer Dietmar , Leithner Andreas , Kretschmer Nadine
Published: November 2023

Myxofibrosarcoma (MFS) is a subtype of soft tissue sarcoma of connective tissue, which is characterized by large intra-tumor heterogeneity. Therapy includes surgical resection. Additional chemotherapy is of limited effect. In this study, we demonstrated the potent anticancer activity of shikonin derivatives in our MFS cellular model of tumor heterogeneity for developing a new therapeutic approach. The impact of shikonin and β,β-dimethylacrylshikonin (DMAS) on viability, apoptotic induction, MAPK phosphorylation, and DNA damage response were analyzed by means of two human MFS cell lines, MUG-Myx2a and MUG-Myx2b, derived from a singular tumor tissue specimen. MFS cells showed a dose-dependent inhibition of cell viability and a significant induction of apoptosis. Treatment with shikonin derivatives caused an inhibition of pSTAT3 and an increase in pAKT, pERK, pJNK, and pp38. DMAS and shikonin inhibited the activation of the two master upstream regulators of the DNA damage response, ATR and ATM. MUG-Myx2b, which contains an additional PTEN mutation, was more sensitive in some targets. These data demonstrate the significant antitumorigenic effect of shikonin derivatives in MFS and highlight the importance of intra-tumor heterogeneity in treatment planning.