bluuebunny/crossref_metadata_2025 huggingface dataset:
Tetrodotoxins Secretion and Voltage-Gated Sodium Channel Adaptation in the Ribbon Worm Kulikovia alborostrata (Takakura, 1898) (Nemertea)
Authors: Vlasenko Anna E. , Kuznetsov Vasiliy G. , Malykin Grigorii V. , Pereverzeva Alexandra O. , Velansky Peter V. , Yakovlev Konstantin V. , Magarlamov Timur Yu.
Published: September 2021

Nemertea is a phylum of marine worms whose members bear various toxins, including tetrodotoxin (TTX) and its analogues. Despite the more than 30 years of studying TTXs in nemerteans, many questions regarding their functions and the mechanisms ensuring their accumulation and usage remain unclear. In the nemertean Kulikovia alborostrata, we studied TTX and 5,6,11-trideoxyTTX concentrations in body extracts and in released mucus, as well as various aspects of the TTX-positive-cell excretion system and voltage-gated sodium (Nav1) channel subtype 1 mutations contributing to the toxins’ accumulation. For TTX detection, an immunohistological study with an anti-TTX antibody and HPLC-MS/MS were conducted. For Nav1 mutation searching, PCR amplification with specific primers, followed by Sanger sequencing, was used. The investigation revealed that, in response to an external stimulus, subepidermal TTX-positive cells released secretions actively to the body surface. The post-release toxin recovery in these cells was low for TTX and high for 5,6,11-trideoxyTTX in captivity. According to the data obtained, there is low probability of the targeted usage of TTX as a repellent, and targeted 5,6,11-trideoxyTTX secretion by TTX-bearing nemerteans was suggested as a possibility. The Sanger sequencing revealed identical sequences of the P-loop regions of Nav1 domains I–IV in all 17 studied individuals. Mutations comprising amino acid substitutions, probably contributing to nemertean channel resistance to TTX, were shown.
Bee Venom Acupuncture Effects on Pain and Its Mechanisms: An Updated Review
Authors: Sung Soo-Hyun , Lee Gihyun
Published: September 2021

Bee venom (BV) is a complex natural toxin that contains various pharmaceutical compounds. Bee venom acupuncture (BVA), involving a BV injection into a certain acupuncture point, has been utilized to relieve a range of pain conditions. Regardless of whether pain is caused by disease or injury, if not effectively treated, pain can exert a detrimental effect on all aspects of life. In the past decade, many researchers have investigated the anti-nociceptive effects of BVA through clinical use and experimental evaluation. This report reviews the existing knowledge on the analgesic effects of BVA, focusing on musculoskeletal pain, inflammatory pain and neuropathic pain, and its analgesic mechanisms. Although further clinical trials are needed to clinical application of experimental results, this review will contribute to the standardization and generalization of BVA.
Expression Analyses of Genes Related to Multixenobiotic Resistance in Mytilus galloprovincialis after Exposure to Okadaic Acid-Producing Dinophysis acuminata
Authors: Martínez-Escauriaza Roi , Lozano Vanessa , Pérez-Parallé M. Luz , Blanco Juan , Sánchez José L. , Pazos Antonio J.
Published: September 2021

The mussel Mytilus galloprovincialis is one of the most important aquaculture species in Europe. Its main production problem is the accumulation of toxins during coastal blooms, which prevents mussel commercialization. P-glycoprotein (ABCB1/MDR1/P-gp) is part of the multixenobiotic resistance system in aquatic organisms, and okadaic acid, the main DSP toxin, is probably a substrate of the P-gp-mediated efflux. In this study, the presence and possible role of P-gp in the okadaic acid detoxification process was studied in M. galloprovincialis. We identified, cloned, and characterized two complete cDNAs of mdr1 and mdr2 genes. MgMDR1 and MgMDR2 predicted proteins had the structure organization of ABCB full transporters, and were identified as P-gp/MDR/ABCB proteins. Furthermore, the expression of mdr genes was monitored in gills, digestive gland, and mantle during a cycle of accumulation-elimination of okadaic acid. Mdr1 significantly increased its expression in the digestive gland and gills, supporting the idea of an important role of the MDR1 protein in okadaic acid efflux out of cells in these tissues. The expression of M. galloprovincialismrp2, a multidrug associated protein (MRP/ABCC), was also monitored. As in the case of mdr1, there was a significant induction in the expression of mrp2 in the digestive gland, as the content of okadaic acid increased. Thus, P-gp and MRP might constitute a functional defense network against xenobiotics, and might be involved in the resistance mechanisms to DSP toxins.
Application of Plant Viruses in Biotechnology, Medicine, and Human Health
Authors: Venkataraman Srividhya , Hefferon Kathleen
Published: September 2021

Plant-based nanotechnology programs using virus-like particles (VLPs) and virus nanoparticles (VNPs) are emerging platforms that are increasingly used for a variety of applications in biotechnology and medicine. Tobacco mosaic virus (TMV) and potato virus X (PVX), by virtue of having high aspect ratios, make ideal platforms for drug delivery. TMV and PVX both possess rod-shaped structures and single-stranded RNA genomes encapsidated by their respective capsid proteins and have shown great promise as drug delivery systems. Cowpea mosaic virus (CPMV) has an icosahedral structure, and thus brings unique benefits as a nanoparticle. The uses of these three plant viruses as either nanostructures or expression vectors for high value pharmaceutical proteins such as vaccines and antibodies are discussed extensively in the following review. In addition, the potential uses of geminiviruses in medical biotechnology are explored. The uses of these expression vectors in plant biotechnology applications are also discussed. Finally, in this review, we project future prospects for plant viruses in the fields of medicine, human health, prophylaxis, and therapy of human diseases.
Detection and Complete Genome Analysis of Circoviruses and Cycloviruses in the Small Indian Mongoose (Urva auropunctata): Identification of Novel Species
Authors: Gainor Kerry , Becker Anne A. M. J. , Malik Yashpal S. , Ghosh Souvik
Published: September 2021

Fecal samples from 76 of 83 apparently healthy small Indian mongooses (Urva auropunctata) were PCR positive with circovirus/cyclovirus pan-rep (replicase gene) primers. In this case, 30 samples yielded high quality partial rep sequences (~400 bp), of which 26 sequences shared maximum homology with cycloviruses from an arthropod, bats, humans or a sheep. Three sequences exhibited maximum identities with a bat circovirus, whilst a single sequence could not be assigned to either genus. Using inverse nested PCRs, the complete genomes of mongoose associated circoviruses (Mon-1, -29 and -66) and cycloviruses (Mon-20, -24, -32, -58, -60 and -62) were determined. Mon-1, -20, -24, -29, -32 and -66 shared <80% maximum genome-wide pairwise nucleotide sequence identities with circoviruses/cycloviruses from other animals/sources, and were assigned to novel circovirus, or cyclovirus species. Mon-58, -60 and -62 shared maximum pairwise identities of 79.90–80.20% with human and bat cycloviruses, which were borderline to the cut-off identity value for assigning novel cycloviral species. Despite high genetic diversity, the mongoose associated circoviruses/cycloviruses retained the various features that are conserved among members of the family Circoviridae, such as presence of the putative origin of replication (ori) in the 5′-intergenic region, conserved motifs in the putative replication-associated protein and an arginine rich region in the amino terminus of the putative capsid protein. Since only fecal samples were tested, and mongooses are polyphagous predators, we could not determine whether the mongoose associated circoviruses/cycloviruses were of dietary origin, or actually infected the host. To our knowledge, this is the first report on detection and complete genome analysis of circoviruses/cycloviruses in the small Indian mongoose, warranting further studies in other species of mongooses.
Erratum: Rose et al. When in Need of an ESCRT: The Nature of Virus Assembly Sites Suggests Mechanistic Parallels between Nuclear Virus Egress and Retroviral Budding. Viruses 2021, 13, 1138
Authors: Rose Kevin M. , Spada Stephanie J. , Hirsch Vanessa M. , Bouamr Fadila
Published: September 2021

The authors wish to make the following erratum to this paper [...]
No Exchange of Picornaviruses in Vietnam between Humans and Animals in a High-Risk Cohort with Close Contact despite High Prevalence and Diversity
Authors: Lu Lu , Ashworth Jordan , Nguyen Dung , Li Kejin , Smith Donald B. , Woolhouse Mark ,   on behalf of the VIZIONS Consortium
Published: September 2021

Hospital-based and community-based ‘high-risk cohort’ studies investigating humans at risk of zoonotic infection due to occupational or residential exposure to animals were conducted in Vietnam, with diverse viruses identified from faecal samples collected from humans, domestic and wild animals. In this study, we focus on the positive-sense RNA virus family Picornaviridae, investigating the prevalence, diversity, and potential for cross-species transmission. Through metagenomic sequencing, we found picornavirus contigs in 23% of samples, belonging to 15 picornavirus genera. Prevalence was highest in bats (67%) while diversity was highest in rats (nine genera). In addition, 22% of the contigs were derived from novel viruses: Twelve phylogenetically distinct clusters were observed in rats of which seven belong to novel species or types in the genera Hunnivirus, Parechovirus, Cardiovirus, Mosavirus and Mupivirus; four distinct clusters were found in bats, belonging to one novel parechovirus species and one related to an unclassified picornavirus. There was no evidence for zoonotic transmission in our data. Our study provides an improved knowledge of the diversity and prevalence of picornaviruses, including a variety of novel picornaviruses in rats and bats. We highlight the importance of monitoring the human–animal interface for possible spill-over events.
Genomic Characterisation of a Highly Divergent Siadenovirus (Psittacine Siadenovirus F) from the Critically Endangered Orange-Bellied Parrot (Neophema chrysogaster)
Authors: Athukorala Ajani , Phalen David , Das Ashutosh , Helbig Karla , Forwood Jade , Sarker Subir
Published: September 2021

Siadenoviruses have been detected in wild and captive birds worldwide. Only nine siadenoviruses have been fully sequenced; however, partial sequences for 30 others, many of these from wild Australian birds, are also described. Some siadenoviruses, e.g., the turkey siadenovirus A, can cause disease; however, most cause subclinical infections. An example of a siadenovirus causing predominately subclinical infections is psittacine siadenovirus 2, proposed name psittacine siadenovirus F (PsSiAdV-F), which is enzootic in the captive breeding population of the critically endangered orange-bellied parrot (OBP, Neophema chrysogaster). Here, we have fully characterised PsSiAdV-F from an OBP. The PsSiAdV-F genome is 25,392 bp in length and contained 25 putative genes. The genome architecture of PsSiAdV-F exhibited characteristics similar to members within the genus Siadenovirus; however, the novel PsSiAdV-F genome was highly divergent, showing highest and lowest sequence similarity to skua siadenovirus A (57.1%) and psittacine siadenovirus D (31.1%), respectively. Subsequent phylogenetic analyses of the novel PsSiAdV-F genome positioned the virus into a phylogenetically distinct sub-clade with all other siadenoviruses and did not show any obvious close evolutionary relationship. Importantly, the resulted tress continually demonstrated that novel PsSiAdV-F evolved prior to all known members except the frog siadenovirus A in the evolution and possibly the ancestor of the avian siadenoviruses. To date, PsSiAdV-F has not been detected in wild parrots, so further studies screening PsSiAdV-F in wild Australian parrots and generating whole genome sequences of siadenoviruses of Australian native passerine species is recommended to fill the siadenovirus evolutionary gaps.
SARS-CoV-2 Infection Modulates ACE2 Function and Subsequent Inflammatory Responses in Swabs and Plasma of COVID-19 Patients
Authors: Gutiérrez-Chamorro Lucía , Riveira-Muñoz Eva , Barrios Clara , Palau Vanesa , Nevot Maria , Pedreño-López Sònia , Senserrich Jordi , Massanella Marta , Clotet Bonaventura , Cabrera Cecilia , Mitjà Oriol , Crespo Marta , Pascual Julio , Riera Marta , Ballana Ester
Published: September 2021

Angiotensin converting enzyme 2 (ACE2) is a host ectopeptidase and the receptor for the SARS-CoV-2 virus, albeit virus-ACE2 interaction goes far beyond viral entry into target cells. Controversial data exists linking viral infection to changes in ACE2 expression and function, which might influence the subsequent induction of an inflammatory response. Here, we tested the significance of soluble ACE2 enzymatic activity longitudinally in nasopharyngeal swabs and plasma samples of SARS-CoV-2 infected patients, along with the induction of inflammatory cytokines. Release of soluble functional ACE2 increases upon SARS-CoV-2 infection in swabs and plasma of infected patients, albeit rapidly decreasing during infection course in parallel with ACE2 gene expression. Similarly, SARS-CoV-2 infection also induced the expression of inflammatory cytokines. These changes positively correlated with the viral load. Overall, our results demonstrate the existence of mechanisms by which SARS-CoV-2 modulates ACE2 expression and function, intracellular viral sensing and subsequent inflammatory response, offering new insights into ACE2 dynamics in the human upper respiratory tract and pointing towards soluble ACE2 levels as a putative early biomarker of infection severity.
Substrate Specificity of SARS-CoV-2 Nsp10-Nsp16 Methyltransferase
Authors: Benoni Roberto , Krafcikova Petra , Baranowski Marek R. , Kowalska Joanna , Boura Evzen , Cahová Hana
Published: September 2021

The ongoing COVID-19 pandemic exemplifies the general need to better understand viral infections. The positive single-strand RNA genome of its causative agent, the SARS coronavirus 2 (SARS-CoV-2), encodes all viral enzymes. In this work, we focused on one particular methyltransferase (MTase), nsp16, which, in complex with nsp10, is capable of methylating the first nucleotide of a capped RNA strand at the 2′-O position. This process is part of a viral capping system and is crucial for viral evasion of the innate immune reaction. In light of recently discovered non-canonical RNA caps, we tested various dinucleoside polyphosphate-capped RNAs as substrates for nsp10-nsp16 MTase. We developed an LC-MS-based method and discovered four types of capped RNA (m7Gp3A(G)- and Gp3A(G)-RNA) that are substrates of the nsp10-nsp16 MTase. Our technique is an alternative to the classical isotope labelling approach for the measurement of 2′-O-MTase activity. Further, we determined the IC50 value of sinefungin to illustrate the use of our approach for inhibitor screening. In the future, this approach may be an alternative technique to the radioactive labelling method for screening inhibitors of any type of 2′-O-MTase.
Prevalence and Molecular Characterization of Human Bocavirus Detected in Croatian Children with Respiratory Infection
Authors: Ljubin-Sternak Sunčanica , Slović Anamarija , Mijač Maja , Jurković Mirna , Forčić Dubravko , Ivković-Jureković Irena , Tot Tatjana , Vraneš Jasmina
Published: September 2021

Human bocavirus (HBoV) 1 is considered an important respiratory pathogen, while the role of HBoV2-4 in clinical disease remains somewhat controversial. Since, they are characterized by a rapid evolution, worldwide surveillance of HBoVs’ genetics is necessary. This study explored the prevalence of HBoV genotypes in pediatric patients with respiratory tract infection in Croatia and studied their phylogeny. Using multiplex PCR for 15 respiratory viruses, we investigated 957 respiratory samples of children up to 18 years of age with respiratory tract infection obtained from May 2017 to March 2021 at two different hospitals in Croatia. Amplification of HBoV near-complete genome or three overlapping fragments was performed, sequenced, and their phylogenetic inferences constructed. HBoV was detected in 7.6% children with a median age of 1.36 years. Co-infection was observed in 82.2% samples. Sequencing was successfully performed on 29 HBoV positive samples, and all belonged to HBoV1. Croatian HBoV1 sequences are closely related to strains isolated worldwide, and no phylogenetic grouping based on mono- or co-infection cases or year of isolation was observed. Calculated rates of evolution for HBoV1 were 10−4 and 10−5 substitutions per site and year. Recombination was not detected among sequences from this study.
Structure-Guided Creation of an Anti-HA Stalk Antibody F11 Derivative That Neutralizes Both F11-Sensitive and -Resistant Influenza A(H1N1)pdm09 Viruses
Authors: Kotani Osamu , Suzuki Yasushi , Saito Shinji , Ainai Akira , Ueno Akira , Hemmi Takuya , Sano Kaori , Tabata Koshiro , Yokoyama Masaru , Suzuki Tadaki , Hasegawa Hideki , Sato Hironori
Published: September 2021

The stalk domain of influenza virus envelope glycoprotein hemagglutinin (HA) constitutes the axis connecting the head and transmembrane domains, and plays pivotal roles in conformational rearrangements of HA for virus infection. Here we characterized molecular interactions between the anti-HA stalk neutralization antibody F11 and influenza A(H1N1)pdm09 HA to understand the structural basis of the actions and modifications of this antibody. In silico structural analyses using a model of the trimeric HA ectodomain indicated that the F11 Fab fragment has physicochemical properties, allowing it to crosslink two HA monomers by binding to a region near the proteolytic cleavage site of the stalk domain. Interestingly, the F11 binding allosterically caused a marked suppression of the structural dynamics of the HA cleavage loop and flanking regions. Structure-guided mutagenesis of the F11 antibody revealed a critical residue in the F11 light chain for the F11-mediated neutralization. Finally, the mutagenesis led to identification of a unique F11 derivative that can neutralize both F11-sensitive and F11-resistant A(H1N1)pdm09 viruses. These results raise the possibility that F11 sterically and physically disturbs proteolytic cleavage of HA for the ordered conformational rearrangements and suggest that in silico guiding experiments can be useful to create anti-HA stalk antibodies with new phenotypes.
RNA-Dependent RNA Polymerase from Heterobasidion RNA Virus 6 Is an Active Replicase In Vitro
Authors: Levanova Alesia A. , Vainio Eeva J. , Hantula Jarkko , Poranen Minna M.
Published: September 2021

Heterobasidion RNA virus 6 (HetRV6) is a double-stranded (ds)RNA mycovirus and a member of the recently established genus Orthocurvulavirus within the family Orthocurvulaviridae. The purpose of the study was to determine the biochemical requirements for RNA synthesis catalyzed by HetRV6 RNA-dependent RNA polymerase (RdRp). HetRV6 RdRp was expressed in Escherichia coli and isolated to near homogeneity using liquid chromatography. The enzyme activities were studied in vitro using radiolabeled UTP. The HetRV6 RdRp was able to initiate RNA synthesis in a primer-independent manner using both virus-related and heterologous single-stranded (ss)RNA templates, with a polymerization rate of about 46 nt/min under optimal NTP concentration and temperature. NTPs with 2′-fluoro modifications were also accepted as substrates in the HetRV6 RdRp-catalyzed RNA polymerization reaction. HetRV6 RdRp transcribed viral RNA genome via semi-conservative mechanism. Furthermore, the enzyme demonstrated terminal nucleotidyl transferase (TNTase) activity. Presence of Mn2+ was required for the HetRV6 RdRp catalyzed enzymatic activities. In summary, our study shows that HetRV6 RdRp is an active replicase in vitro that can be potentially used in biotechnological applications, molecular biology, and biomedicine.
Development and Validation of an ELISA for the Detection of Bluetongue Virus Serotype 4-Specific Antibodies
Authors: Bréard Emmanuel , Turpaud Mathilde , Beaud Georges , Postic Lydie , Fablet Aurore , Beer Martin , Sailleau Corinne , Caignard Grégory , Viarouge Cyril , Hoffmann Bernd , Vitour Damien , Zientara Stéphan
Published: September 2021

In this article, we describe the development and evaluation of a double antigen sandwich enzyme-linked immunosorbent assay (ELISA) able to detect serotype 4-specific antibodies from BTV-4 infected or vaccinated animals using a recombinant BTV-4 VP2 protein. The coding sequence of VP2 was inserted into a pVote plasmid by recombination in the Gateway® cloning system. Vaccinia virus (VacV) was used as a vector for the expression of the recombinant VP2. After production in BSR cells, recombinant VP2 was purified by immunoprecipitation using a FLAG tag and then used both as the coated ELISA antigen and as the HRP-tagged conjugate. The performance of the ELISA was evaluated with 1186 samples collected from BTV negative, infected or vaccinated animals. The specificity and sensitivity of the BTV-4 ELISA were above the expected standards for the detection of anti-BTV-4 VP2 antibodies in animals reared in Europe or in the Mediterranean basin. Cross-reactions were observed with reference sera for serotypes 10 and 20, and to a lesser extent with serotypes 12, 17 and 24, due to their genetic proximity to serotype 4. Nevertheless, these serotypes have never been detected in Europe and the Mediterranean area. This ELISA, which requires only the production of a recombinant protein, can be used to detect BTV serotype 4-specific antibodies and is therefore an attractive alternative diagnostic method to serum neutralization.
Bringing Preventive RSV Monoclonal Antibodies to Infants in Low- and Middle-Income Countries: Challenges and Opportunities
Authors: Ananworanich Jintanat , Heaton Penny M.
Published: September 2021

Respiratory syncytial virus (RSV) is the leading cause of acute lower respiratory tract infections (LRTIs) in infants. Most deaths occur in infants under 3 months old, and those living in low and middle-income countries (LMICs). There are no maternal or infant RSV vaccines currently approved. An RSV monoclonal antibody (mAb) could fill the gap until vaccines are available. It could also be used when a vaccine is not given, or when there is insufficient time to vaccinate and generate an antibody response. The only currently approved RSV mAb, palivizumab, is too costly and needs monthly administration, which is not possible in LMICs. It is imperative that a safe, effective, and affordable mAb to prevent severe RSV LRTI be developed for infants in LMICs. Next generation, half-life extended mAbs in clinical development, such as nirsevimab, show promise in protecting infants against RSV LRTI. Given that a single dose could cover an entire 5-month season, there is an opportunity to make RSV mAbs affordable for LMICs by investing in improvements in manufacturing efficiency. The challenges of using RSV mAbs in LMICs are the complexities of integrating them into existing healthcare delivery programs and surveillance systems, both of which are needed to define seasonal patterns, and monitor for escape mutants. Collaboration with key stakeholders such as the World Health Organization and Gavi, the Vaccine Alliance, will be essential for achieving this goal.
Apron and Cutoff Wall Scour Protection for Piano Key Weirs
Authors: Lantz Wyatt , Crookston Brian Mark , Palermo Michele
Published: September 2021

Piano key (PK) weirs are used in a variety of flow control structure applications, including spillway crests and open channel diversion structures. However, to the best of authors’ knowledge, structure-specific design guidance for scour mitigation is still needed. To fill this gap of knowledge, a systematic experimental campaign was conducted by testing different configurations of horizontal aprons with a cutoff wall. Protection structures were located at the toe of the PK weir. Namely, experiments were performed at large-scale to assess the effect of three apron lengths on downstream scour hole geometry under different hydraulic conditions. It was observed that a horizontal apron deflects the plunging jets originating from the PK weir, thus significantly reducing scour. Experimental evidence allowed corroboration that significant scour depth reduction occurs for an apron length 1.5 times the weir height, with longer aprons found to provide marginal benefits. Finally, also provided herein are tools to estimate the main scour characteristics and help practitioners in optimizing apron design.
Uncertainty of Intensity-Duration-Frequency Curves Due to Adoption or Otherwise of the Temperature Climate Variable in Rainfall Disaggregation
Authors: Fadhel Sherien , Al Aukidy Mustafa , Saleh May Samir
Published: September 2021

Most areas around the world lack fine rainfall records which are needed to derive Intensity-Duration-Frequency (IDF) curves, and those that are available are in the form of daily data. Thus, the disaggregation of rainfall data from coarse to fine temporal resolution may offer a solution to that problem. Most of the previous studies have adopted only historical rainfall data as the predictor to disaggregate daily rainfall data to hourly resolution, while only a few studies have adopted other historical climate variables besides rainfall for such a purpose. Therefore, this study adopts and assesses the performance of two methods of rainfall disaggregation one uses for historical temperature and rainfall variables while the other uses only historical rainfall data for disaggregation. The two methods are applied to disaggregate the current observed and projected modeled daily rainfall data to an hourly scale for a small urban area in the United Kingdom. Then, the IDF curves for the current and future climates are derived for each case of disaggregation and compared. After which, the uncertainties associated with the difference between the two cases are assessed. The constructed IDF curves (for the two cases of disaggregation) agree in the sense that they both show that there is a big difference between the current and future climates for all durations and frequencies. However, the uncertainty related to the difference between the results of the constructed IDF curves (for the two cases of disaggregation) for each climate is considerable, especially for short durations and long return periods. In addition, the projected and current rainfall values based on disaggregation case which adopts historical temperature and rainfall variables were higher than the corresponding projections and current values based on only rainfall data for the disaggregation.
Emerging Contaminants: An Overview of Recent Trends for Their Treatment and Management Using Light-Driven Processes
Authors: Lee Brandon Chuan Yee , Lim Fang Yee , Loh Wei Hao , Ong Say Leong , Hu Jiangyong
Published: September 2021

The management of contaminants of emerging concern (CECs) in water bodies is particularly challenging due to the difficulty in detection and their recalcitrant degradation by conventional means. In this review, CECs are characterized to give insights into the potential degradation performance of similar compounds. A two-pronged approach was then proposed for the overall management of CECs. Light-driven oxidation processes, namely photo/Fenton, photocatalysis, photolysis, UV/Ozone were discussed. Advances to overcome current limitations in these light-driven processes were proposed, focusing on recent trends and innovations. Light-based detection methodology was also discussed for the management of CECs. Lastly, a cost–benefit analysis on various light-based processes was conducted to access the suitability for CECs degradation. It was found that the UV/Ozone process might not be suitable due to the complication with pH adjustments and limited light wavelength. It was found that EEO values were in this sequence: UV only > UV/combination > photocatalyst > UV/O3 > UV/Fenton > solar/Fenton. The solar/Fenton process has the least computed EEO < 5 kWh m−3 and great potential for further development. Newer innovations such as solar/catalyst can also be explored with potentially lower EEO values.
Assimilation of Piezometric Data to Calibrate Parsimonious Daily Hydrological Models
Authors: Flinck Axel , Folton Nathalie , Arnaud Patrick
Published: September 2021

Low water levels are a seasonal phenomenon, which can be long, short, and more or less intense, affecting entire watercourses. This phenomenon has become a concern for many countries who seek better understanding of the processes that affect it and learn how to optimally manage water resources (pumping, irrigation). Consequently, a lumped rainfall model at daily time step (GR) has been defined, calibrated, and regionalised over French territories. The input data come from SAFRAN, the distributed mesoscale atmospheric analysis system, which provides daily solid and liquid precipitation and temperature data throughout the French territory. This model could be improved, in particular to more accurately simulate the hydrological response of watersheds interacting with groundwater. The idea is to use piezometric data from the ADES bank, available in France, and to use it for the calibration phase of the hydrological model. The analysis was carried out across ten French catchments that are representative of various hydrometeorological behaviours and are located in a diverse hydrogeological context. Each catchment must be represented by a piezometer that closely represents the main aquifer that interacts with the basin. This piezometer is located on part of the watershed that is most covered in terms of its drainage network, and closest to its outlet. Different signal processing methods are used to characterise the relationship between the fluctuation of river flow, piezometric levels and rainfall time series. Potential processing methods will be carried out in the temporal domain. To quantify groundwater table inertia and that of the catchment area, correlograms were calculated from daily chronicles of flows and piezometric levels. A cross-correlatory analysis was set up to see, in more detail, the correlations between the flow rates (especially base flows) and piezometric level time series. This type of analysis makes it possible to study relationships between various observations, and tests were carried out to take this information into account during the phase of the calibration of hydrological model parameters. These different analyses will hopefully help us to use piezometric data to consolidate the quality and robustness of the modelling.
Assessment of Arsenic Levels in Water, Sediment, and Human Hair around Ie Seu’um Geothermal Manifestation Area, Aceh, Indonesia
Authors: Irnawati Irnawati , Idroes Rinaldi , Zulfiani Utari , Akmal Muslim , Suhartono Eko , Idroes Ghazi Mauer , Muslem Muslem , Lala Andi , Yusuf Muhammad , Saiful Saiful , Suhud Khairi , Safitri Eka , Jalil Zulkarnain
Published: September 2021

The concentration of arsenic in water, sediment, and resident hair in the Ie Seu’um geothermal manifestation area has been analyzed using the Atomic Graphite Furnace Atomic Absorption Spectrophotometry (GF-AAS) method. Sediment and hair samples were prepared by wet digestion using an acid solution. The measurement results were validated by linearity, Limit of Detection (LoD), Limit of Quantification (LoQ), Relative Standard Deviation (%RSD), and %Recovery. The validation test showed that this method is very linear, sensitive, accurate, and precise with a correlation coefficient of 0.9999, LoD of 0.009 μg/L, LoQ of 0.027 μg/L, recovery values of 89.117–101.027% for hair samples and 88.337–105.175% for sediment samples, and RSD of 1.067%. The sample test results showed that the hot springs contained the highest arsenic with levels of 166.73 ± 0.0081 μg/L (IS1). In comparison, the water samples with the lowest arsenic levels were in the rivers that had not been in direct contact with the hot water (IS2) flow, which is 0.80 ± 0.0036 μg/L. All the river water samples that had been in contact with hot water were boreholes, and the resident boreholes located around hot water streams/rivers contaminated with arsenic were above the threshold. Only the river water that had not been in contact with hot water, refill water, and borehole waters outside the hot water flow were detected to be below the threshold level. All the sediment samples showed arsenic contamination, with levels ranging from 2.56–6.86 mg/kg, and it was still within the normal limits recommended by the National Oceanic and Atmospheric Administration (NOAA). Arsenic exposures in communities living around the Ie Seu’um geothermal area, Mesjid Raya Sub-District, Aceh Besar District, Aceh Province, were very high, where 9 out of 10 respondents were positive for arsenic with levels ranging from 54.59–164.57 mg/kg, which was already above the threshold set by the researchers.
Unexpected Diversity of Feeding Modes among Chisel-Mouthed Ethiopian Labeobarbus (Cyprinidae)
Authors: Levin Boris A. , Komarova Aleksandra S. , Rozanova Oksana L. , Golubtsov Alexander S.
Published: September 2021

Trophic resource partitioning is one of the main drivers of adaptive radiation. The evolutionary diversification of large African barbs, the genus Labeobarbus, seems to be related to mouth polymorphism. The chisel-mouthed or scraping phenotype has repeatedly evolved within Labeobarbus. At least five ecomorphs with a scraping mouth morphology were detected in the waters of the Ethiopian Highlands and can be provisionally classified into two groups: (i) “Varicorhinus”-like, and (ii) “Smiling”-like. Previously, all Labeobarbus with a scraping-mouth morphology were considered to be periphyton feeders. Using data on morphology, diet and stable isotope ratios (C and N), we addressed the question: does a scraping-mouth morphology predict feeding on periphyton? Our study revealed that five scraper ecomorphs exhibited three main feeding modes: (i) periphyton-eating, (ii) herbivory–detritivory, and (iii) insectivory. Two cases of the parallel divergence of sympatric ecomorphs with distinct feeding modes (herbivory–detritivory vs. insectivory) were revealed in two geographically isolated basins. A significant difference in δ15N values was detected among sympatric scraper ecomorphs. A periphytonophagous scraper was rich in δ15N values that are comparable with those in sympatric piscivorous fish. This data sheds light on the possibility of the utilization of periphyton as a protein-rich food by fishes.
Multifractal Analysis of River Networks under the Background of Urbanization in the Yellow River Basin, China
Authors: Wang Jinxin , Qin Zilong , Shi Yan , Yao Jing
Published: September 2021

Multifractal theory provides an effective method for the scientific quantification of the river network features. This method has been applied to estimate river network structure in previous research, but there are few temporal and spatial analysis studied for large river basins based on multifractals. In this study, the Yellow River Basin and its nine provinces were selected as the study area, and the generalized fractal dimension and multifractal spectrum of the study area during the period 2000–2020 were calculated and analyzed. We analyzed the response relationship between the river network changes and the urbanization process. Results indicated that the river network of the study area exhibited obvious multifractal properties, which were mainly affected by dense river networks. The complexity of the studied river network has shown a tendency to decrease over time. The rate of change in the river network structure has a high positive correlation with urbanization, that is, the higher the rate of urbanization, the greater the impact on the river network structure. Additionally, the changes of the river network structure are more affected by urbanization during the rapid urbanization stage. We applied multifractal analysis to study the river network structure changes, which is of great significance for scientifically quantifying fluvial characteristics and studying the development and evolution of river networks.
Identifying Hub Wastewater Propagation Chains in China’s National Economic System: A Model Coupled Input-Output Analysis with Graphical Theory
Authors: Li Xuefeng , Liu Xiuli
Published: September 2021

Wastewater propagation chains (WPCs) measure inter-sector average propagation lengths (APL) of wastewater discharge. To achieve sustainable wastewater management, one needs to understand the propagation mechanisms by identifying WPCs at a national level over time. However, the traditional model of identifying WPCs is prone to retaining APLs with lower values but larger wastewater discharge intensities, ignoring many linkages whereby intensities are less than a preset threshold. Nevertheless, these overlooked linkages are valuable in understanding wastewater propagation mechanisms. This study proposed a new model coupled input-output analysis with the graphical theory, called the average propagation lengths-hub covariance graph (APL-HCG). This model can investigate WPCs where the closeness of sector linkages exceeds the preset thresholds. Furthermore, it is capable of retaining linkages for identifying hub wastewater propagation chains (HWPCs). Based on APL-HCG, the resultant HWPCs are decomposed as separated sub-chains which are basically composed of linkages among certain significant sectors belonging to the secondary industry or the tertiary industry. Scenario analyses show that HWPCs are effective in reducing wastewater discharge in the national economic system. The total wastewater discharge would decrease by 1.36%, 2.53%, 2.46%, and 2.11% if we reduced 10% of the final demand of all sectors in HWPCs in 2002, 2007, 2012, and 2017. The APL-HCG model outperforms the traditional model on WPCs by 0.14%, 1.61%, 0.47%, and 0.10%, respectively. The APL-HCG model is 0.21%, 0.68%, 0.70%, and 0.35% better than the scenario of random sampling with the number of sectors equal to HWPCs, respectively. Certain policy implications were provided to reduce wastewater effectively at the national level.
An Integrated Aquifer Management Approach for Aridification-Affected Agricultural Area, Shengeldy-Kazakhstan
Authors: Mirlas Vladimir , Makyzhanova Assyl , Kulagin Vitaly , Kuldeev Erghan , Anker Yaakov
Published: September 2021

Ongoing water-resource depletion is a common trend in southeastern Kazakhstan and in most of Central Asia, making the use of drainage water for freshwater preservation and groundwater recharge a key strategy for sustainable agriculture. Since the Ily River inflow began to decrease, groundwater levels in the Shengeldy study area site have fallen below the drainage pipes. As such, our main research hypothesis was that owing to drainage infiltration, the regional shallow aquifer can be used as an effective additional water source for moistening crop root systems during the irrigation period. The MODFLOW groundwater flow model was used to simulate and quantitatively assess the combined hydrogeological and irrigation conditions of artificial groundwater recharge both from the subsurface drainage and as an additional source for irrigation. The field study showed that the additional groundwater table elevation will reach approximately 1.5 m under the field drainage system and that the additional groundwater recharge influence zone will develop up to 300–350 m from the drains. The MODFLOW simulation together with full-scale experimental studies suggests that under certain conditions drainage water can be applied both as an additional source of irrigation and for aquifer sustainable maintenance.
Optimisation of Various Physicochemical Variables Affecting Molybdenum Bioremediation Using Antarctic Bacterium, Arthrobacter sp. Strain AQ5-05
Authors: Darham Syazani , Syed-Muhaimin Sharifah Nabilah , Subramaniam Kavilasni , Zulkharnain Azham , Shaharuddin Noor Azmi , Khalil Khalilah Abdul , Ahmad Siti Aqlima
Published: September 2021

The versatility of a rare metal, molybdenum (Mo) in many industrial applications is one of the reasons why Mo is currently one of the growing environmental pollutants worldwide. Traces of inorganic contaminants, including Mo, have been discovered in Antarctica and are compromising the ecosystem. Bioremediation utilising bacteria to transform pollutants into a less toxic form is one of the approaches for solving Mo pollution. Mo reduction is a process of transforming sodium molybdate with an oxidation state of 6+ to Mo-blue, an inert version of the compound. Although there are a few Mo-reducing microbes that have been identified worldwide, only two studies were reported on the microbial reduction of Mo in Antarctica. Therefore, this study was done to assess the ability of Antarctic bacterium, Arthrobacter sp. strain AQ5-05, in reducing Mo. Optimisation of Mo reduction in Mo-supplemented media was carried out using one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. Through OFAT, Mo was reduced optimally with substrate concentration of sucrose, ammonium sulphate, and molybdate at 1 g/L, 0.2 g/L, and 10 mM, respectively. The pH and salinity of the media were the best at 7.0 and 0.5 g/L, respectively, while the optimal temperature was at 10 °C. Further optimisation using RSM showed greater Mo-blue production in comparison to OFAT. The strain was able to stand high concentration of Mo and low temperature conditions, thus showing its potential in reducing Mo in Antarctica by employing conditions optimised by RSM.
Spontaneous Imbibition in a Fractal Network Model with Different Wettabilities
Authors: Cai Shaobin , Zhang Li , Kang Lixin , Yang Yongfei , Jing Wenlong , Zhang Lei , Xu Chao , Sun Hai , Sajjadi Mozhdeh
Published: September 2021

In this work, we derived a mathematical model for spontaneous imbibition in a Y-shaped branching network model. The classic Lucas–Washburn equation was used for modeling the imbibition process occurring in the Y-shape model. Then, a mathematical model for the Newtonian fluid’s imbibition was derived to reveal the relationship between dimensionless imbibition time and length ratio, radius ratio, and wetting strength. The dimensionless imbibition time in the model was adopted to compare with that of the capillary bundle model. Different length and radius ratios were considered in the adjacent two-stage channels, and different wettabilities were considered in the different branches. The optimal radius ratio, length ratio, and wetting strength were calculated under the condition of the shortest imbibition time. In addition, the shortest dimensionless imbibition time of the three-stage Y-shaped branching network model was calculated when the wettability changes randomly. The results indicate that the imbibition time changed mostly when the wettability of the second branch changed, and the second branch was the most sensitive to wettability in the model.
A Study on the Flux of Total Suspended Matter in the Padma River in Bangladesh Based on Remote-Sensing Data
Authors: Zheng Zhuoqi , Wang Difeng , Gong Fang , He Xianqiang , Bai Yan
Published: September 2021

The flux of total suspended matter (TSM), FTSM, output by several large rivers in Asia, has been in decline due to human activities. As the estuary of the Ganges–Brahmaputra River, the Padma River transports a significant amount of suspended matter (SM) to the Bay of Bengal each year. In this study, the TSM concentration (CTSM) and FTSM in the Padma River in the period 1991–2019 were calculated based on the data acquired by the Landsat series satellites and an empirical TSM algorithm model for large, high-turbidity rivers. The results showed that the maximum and minimum FTSM values (318 ± 62 and 73 ± 29 mt, respectively) in the Padma River occurred in 2011 and 2015, respectively. On average, FTSM in the Padma River decreased at an annual rate of 3.3 mt (p < 0.01). The impact of human activities on CTSM contributed more significantly to the changes in FTSM (R = 0.76) than natural factors (R = 0.44). Due to a lack of water conservancy facilities within the river basin, changes in the water and soil retention capacity due to the changes in vegetation coverage were an important human factor (R = −0.79).
The Significance of Hydrogen and Oxygen Stable Isotopes in the Water Vapor Source in Dingxi Area
Authors: Wu Xixi , Chen Fenli , Liu Xueyuan , Wang Shengjie , Zhang Mingjun , Zhu Guofeng , Zhou Xin , Chen Jufan
Published: September 2021

Deuterium excess and stable oxygen isotopes in precipitation have been widely applied to trace the source of water vapor. In this study, hydrogen and oxygen isotope analyses of samples were collected on seven sampling stations in Dingxi area from April 2019 to April 2020. The seasonal variation of hydrogen and oxygen stable isotopes as well as the d-excess indicate that the source of water vapor in Dingxi area is mostly from a single source. However, there are different sources of water vapor in the summer. Meanwhile, water vapor sources were analyzed using the Lagrange algorithm, indicating two different principal water vapor sources for precipitation in the area: some locally recycled water vapor in summer and autumn, and most water vapor from the westerly belt. Further studies using the PSCF and CWT analysis methods show that the locally recycled water vapor contributes more to its precipitation in the northwest of Dingxi area.
High-Resolution Spatiotemporal Trend Analysis of Precipitation Using Satellite-Based Products over the United Arab Emirates
Authors: Hussein Khalid A. , Alsumaiti Tareefa S. , Ghebreyesus Dawit T. , Sharif Hatim O. , Abdalati Waleed
Published: September 2021

Current water demands are adequately satisfied in the United Arab Emirates (UAE) with the available water resources. However, the changing climate and growing water demand pose a great challenge for water resources managers in the country. Hence, there is a great need for management strategies and policies to use the most accurate information regarding water availability. Understanding the frequency and the short- and long-term trends of the precipitation by employing high-resolution data in both the spatial and temporal domains can provide invaluable information. This study examines the long-term precipitation trends over the UAE using 17 years of data from three of the most highly cited satellite-based precipitation products and rain gauge data observed at 18 stations. The UAE received, on average, 42, 51, and 120 wet hours in a year in the 21st century as recorded by CMORPH, PERSIANN, and IMERG, respectively. The results show that the areal average annual precipitation of the UAE is significantly lower in the early 21st century than that of the late 20th century, even though it shows an increasing trend by all the products. The Mann–Kendall trend test showed positive trends in six rain gauge stations and negative trends in two stations out of 18 stations, all of which are located in the wetter eastern part of the UAE. Results indicate that satellite products have great potential for improving the spatial aspects of rainfall frequency analysis and can complement rain gauge data to develop rainfall intensity–duration–frequency curves in a very dry region, where the installation of dense rain gauge networks is not feasible.