bluuebunny/crossref_metadata_2025 huggingface dataset:
Within-host genomic evolution of methicillin-resistant Staphylococcus aureus in long-term carriers
Authors: Larsen Tine Graakjær , Samaniego Castruita Jose Alfredo , Worning Peder , Westh Henrik , Bartels Mette Damkjær
Published: January 2024

Assessing the genomic evolution of Staphylococcus aureus can help us understand how the bacteria adapt to its environment. In this study, we aimed to assess the mutation rate within 144 methicillin-resistant Staphylococcus aureus (MRSA) carriers with a carriage time from 4 to 11 years, including some carriers who belonged to the same households. We found that 23 of the 144 individuals had completely different MRSA types over time and were therefore not long-term carriers of the same MRSA. From the remaining 121 individuals, we performed whole-genome sequencing (WGS) on 424 isolates and then compared these pairwise using core genome multilocus sequence typing (cgMLST) and single-nucleotide polymorphism (SNP) analyses. We found a median within-host mutation rate in long-term MRSA carriers of 4.9 (3.4–6.9) SNPs/genome/year and 2.7 (1.8–4.2) allelic differences/genome/year, when excluding presumed recombination. Furthermore, we stratified the cohort into subgroups and found no significant difference between the median mutation rate of members of households, individuals with presumed continued exposure, e.g., from travel and persons without known continued exposure. Finally, we found that SNPs occurred at random within the genes in our cohort.

Key points

• Median mutation rate within long-term MRSA carriers of 4.9 (3.4–6.9) SNPs/genome/year

• Similar median mutation rates in subgroups (households, travelers)

• No hotspots for SNPs within the genome
Identification of a novel monocyclic carotenoid and prediction of its biosynthetic genes in Algoriphagus sp. oki45
Authors: Takatani Naoki , Maoka Takashi , Sawabe Tomoo , Beppu Fumiaki , Hosokawa Masashi
Published: January 2024

Bacteria belonging to the genus Algoriphagus have been isolated from various sources, such as Antarctic sea ice, seawater, and sediment, and some strains are known to produce orange to red pigments. However, the pigment composition and biosynthetic genes have not been fully elucidated. A new red-pigmented Algoriphagus sp. strain, oki45, was isolated from the surface of seaweed collected from Senaga-Jima Island, Okinawa, Japan. Genome comparison revealed oki45’s average nucleotide identity of less than 95% to its closely related species, Algoriphagus confluentis NBRC 111222 T and Algoriphagus taiwanensis JCM 19755 T. Comprehensive chemical analyses of oki45’s pigments, including 1H and 13C nuclear magnetic resonance and circular dichroism spectroscopy, revealed that the pigments were mixtures of monocyclic carotenoids, (3S)-flexixanthin ((3S)-3,1′-dihydroxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one) and (2R,3S)-2-hydroxyflexixanthin ((2R,3S)-2,3,1′-trihydroxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one); in particular, the latter compound was new and not previously reported. Both monocyclic carotenoids were also found in A. confluentis NBRC 111222 T and A. taiwanensis JCM 19755 T. Further genome comparisons of carotenoid biosynthetic genes revealed the presence of eight genes (crtE, crtB, crtI, cruF, crtD, crtYcd, crtW, and crtZ) for flexixanthin biosynthesis. In addition, a crtG homolog gene encoding 2,2ʹ-β-hydroxylase was found in the genome of the strains oki45, A. confluentis NBRC 111222 T, and A. taiwanensis JCM 19755 T, suggesting that the gene is involved in 2-hydroxyflexixanthin synthesis via 2-hydroxylation of flexixanthin. These findings expand our knowledge of monocyclic carotenoid biosynthesis in Algoriphagus bacteria.

Key points

• Algoriphagus sp. strain oki45 was isolated from seaweed collected in Okinawa, Japan.

• A novel monocyclic carotenoid 2-hydroxyflexixanthin was identified from strain oki45.

• Nine genes for 2-hydroxyflexixanthin biosynthesis were found in strain oki45 genome.

Graphical Abstract
Activity of thonningianin A against Candida albicans in vitro and in vivo
Authors: Wang Hui , Li Hui , Liu ZhiWei , Zhu ZhenYu , Cao YingYing
Published: January 2024

Fungal infections are increasing rapidly, and antifungal agents used in clinics are limited. Therefore, novel antifungal agents with high efficiency are urgently required. In this study, we investigated the antifungal activity of thonningianin A (THA), a natural compound that is widely found in plants. We first determined the activity of THA against Candida albicans, one of the most common fungal pathogens, and found that THA showed antifungal activity against all C. albicans tested, including several fluconazole-resistant isolates. THA also inhibits the growth of non-Candida albicans species. In addition, THA displayed antibiofilm activity and could not only inhibit biofilm formation but also destroy mature biofilms. The in vivo antifungal efficacy of THA was confirmed in a Galleria mellonella infection model. Further studies revealed that THA could enhance intracellular reactive oxygen species (ROS) production and regulate the transcription of several redox-related genes. Specifically, caspase activity and expression of CaMCA1, a caspase-encoding gene in C. albicans, were remarkably increased upon THA treatment. Consistent with this, in the presence of THA, the Camca1 null mutant displayed higher survival rates and reduced caspase activity compared to the wild-type or CaMCA1-reintroduced strains, indicating an important role of CaMCA1 in the antifungal activity of THA. Taken together, our results indicate that THA possesses excellent antifungal activity and may be a promising novel antifungal candidate.

Key points

• THA exhibits activity against Candida species, including fluconazole-resistant isolates

• THA inhibits biofilm formation and destroys mature biofilm

• Elevated ROS production and CaMCA1-mediated caspase activity are involved in the antifungal mechanisms of THA
Adrenal Vein Sampling: Does the Location of the Non-adrenal Venous Sample Matter?
Authors: Wernig Florian , Dunin-Borkowska Aleksandra , Frisiras Angelos , Khoo Bernard , Todd Jeannie , Di Marco Aimee , Palazzo F. Fausto , Barnes Sophie C. , Tan Tricia M. , Meeran Karim , Alsafi Ali
Published: January 2024

Purpose

Adrenal vein sampling (AVS) is used to lateralise and differentiate unilateral from bilateral aldosterone production in primary aldosteronism. The adrenal venous samples are standardised to a peripheral or low inferior vena cava (IVC) sample and compared. It is unknown whether the location of the non-adrenal sample affects the results. This study compares AVS results standardised to the low IVC and right external iliac vein (REIV).

Methods

Patients who underwent AVS between March 2021 and May 2023 were included. All procedures were undertaken by a single operator (AA). Demographic data and AVS results were collected from patients’ electronic records. Catheterisation success and lateralisation were assessed using both low IVC and REIV samples. Equivalence test was used to compare the cortisol and aldosterone levels.

Results

Eighty-one patients, (M: F = 38:43), aged between 29 and 74 were included. Bilateral successful adrenal vein cannulation was achieved in 79/81 (97.5%) cases. The mean cortisol levels from the REIV were statistically equivalent although there was a small and not biologically significant difference from the low IVC (respective geometric means 183 nmol/l vs. 185 nmol/l, p = 0.015). This small difference in cortisol may be due to accessory adrenal venous drainage into the IVC. The aldosterone and aldosterone/cortisol ratios were statistically equivalent. There was no discordance in selectivity or lateralisation when the IVC or REIV measurements were used.

Conclusion

The IVC and REIV samples may be used interchangeably during AVS.

Graphical Abstract
Epicardial adipose tissue dispersion at CT and recurrent atrial fibrillation after pulmonary vein isolation
Authors: Huber Adrian Thomas , Fankhauser Severin , Wittmer Severin , Chollet Laureve , Lam Anna , Maurhofer Jens , Madaffari Antonio , Seiler Jens , Servatius Helge , Haeberlin Andreas , Noti Fabian , Brugger Nicolas , von Tengg-Kobligk Hendrik , Gräni Christoph , Roten Laurent , Tanner Hildegard , Reichlin Tobias
Published: January 2024

Objectives

Epicardial adipose tissue (EAT) remodeling is associated with atrial fibrillation (AF). Left atrial (LA) EAT dispersion on cardiac CT is a non-invasive imaging biomarker reflecting EAT heterogeneity. We aimed to investigate the association of LA EAT dispersion with AF recurrence after pulmonary vein isolation (PVI).

Methods

In a prospective registry of consecutive patients undergoing first PVI, mean EAT attenuation values were measured on contrast-enhanced cardiac CT scans in Hounsfield units (HU) within low (− 195 to − 45 HU) and high (− 44 to − 15 HU) threshold EAT compartments around the left atrium (LA). EAT dispersion was defined as the difference between the mean HU values within the two EAT compartments. Continuous variables were compared between groups using the Mann–Whitney U test and cox proportional hazard models were used to calculate hazard ratios of predictors of 1-year AF recurrence.

Results

A total of 208 patients were included, 135 with paroxysmal AF and 73 with persistent AF. LA EAT dispersion was significantly larger in patients with persistent compared to paroxysmal AF (52.6 HU vs. 49.9 HU; p = 0.001). After 1 year of follow-up, LA EAT dispersion above the mean (> 50.8 HU) was associated with a higher risk of AF recurrence (HR 2.3, 95% CI 1.5–3.6; p < 0.001). It retained its predictive value when corrected for age, sex, body mass index, LA volume, and AF type (HR 2.8, 95% CI 1.6–4.6; p < 0.001).

Conclusion

A larger LA EAT dispersion on contrast-enhanced cardiac CT scans, reflecting EAT heterogeneity, is independently associated with AF recurrence after PVI.

Clinical relevance statement

Based on LA EAT dispersion assessment, a more accurate risk stratification and patient selection may be possible based on a pre-procedural cardiac CT when planning PVI.

Key Points

• Epicardial adipose tissue (EAT) remodeling is associated with atrial fibrillation (AF).

• A larger left atrial EAT dispersion in a pre-procedural cardiac CT was associated with a higher 1-year AF recurrence risk after pulmonary vein isolation.

• A pre-procedural cardiac CT with left atrial EAT dispersion assessment may provide a more accurate risk stratification and patient selection for PVI.
Myocardial strain assessment in the human fetus by cardiac MRI using Doppler ultrasound gating and feature tracking
Authors: Dargahpour Barough Maryam , Tavares de Sousa Manuela , Hergert Bettina , Fischer Roland , Huber Lukas , Seliger Jan Moritz , Kaul Michael Gerhard , Adam Gerhard , Herrmann Jochen , Bannas Peter , Schoennagel Bjoern P.
Published: January 2024

Objectives

Assessment of myocardial strain by feature tracking magnetic resonance imaging (FT-MRI) in human fetuses with and without congenital heart disease (CHD) using cardiac Doppler ultrasound (DUS) gating.

Methods

A total of 43 human fetuses (gestational age 28–41 weeks) underwent dynamic cardiac MRI at 3 T. Cine balanced steady-state free-precession imaging was performed using fetal cardiac DUS gating. FT-MRI was analyzed using dedicated post-processing software. Endo- and epicardial contours were manually delineated from fetal cardiac 4-chamber views, followed by automated propagation to calculate global longitudinal strain (GLS) of the left (LV) and right ventricle (RV), LV radial strain, and LV strain rate.

Results

Strain assessment was successful in 38/43 fetuses (88%); 23 of them had postnatally confirmed diagnosis of CHD (e.g., coarctation, transposition of great arteries) and 15 were heart healthy. Five fetuses were excluded due to reduced image quality. In fetuses with CHD compared to healthy controls, median LV GLS (− 13.2% vs. − 18.9%; p < 0.007), RV GLS (− 7.9% vs. − 16.2%; p < 0.006), and LV strain rate (1.4 s−1 vs. 1.6 s−1; p < 0.003) were significantly higher (i.e., less negative). LV radial strain was without a statistically significant difference (20.7% vs. 22.6%; p = 0.1). Bivariate discriminant analysis for LV GLS and RV GLS revealed a sensitivity of 67% and specificity of 93% to differentiate between fetuses with CHD and healthy fetuses.

Conclusion

Myocardial strain was successfully assessed in the human fetus, performing dynamic fetal cardiac MRI with DUS gating. Our study indicates that strain parameters may allow for differentiation between fetuses with and without CHD.

Clinical relevance statement

Myocardial strain analysis by cardiac MRI with Doppler ultrasound gating and feature tracking may provide a new diagnostic approach for evaluation of fetal cardiac function in congenital heart disease.

Key Points

• MRI myocardial strain analysis has not been performed in human fetuses so far.

• Myocardial strain was assessed in human fetuses using cardiac MRI with Doppler ultrasound gating.

• MRI myocardial strain may provide a new diagnostic approach to evaluate fetal cardiac function.
The impact of replacing milk with plant-based alternatives on iodine intake: a dietary modelling study
Authors: Nicol Katie , Nugent Anne P. , Woodside Jayne V. , Hart Kathryn H. , Bath Sarah C.
Published: January 2024

Purpose

Cow’s milk is the primary source of iodine in the UK, but consumption of plant-based milk alternatives (PBMA) is increasing and these products are often not fortified with iodine. We evaluated the impact that replacing current milk consumption with PBMA would have on iodine intake.

Methods

We used data from the National Diet and Nutrition Survey (2016–2019) for children (1.5–10 years), girls 11–18 years, and women of reproductive age (WRA). We used a dietary modelling approach with scenarios using brand-level iodine-fortification data (0, 13, 22.5, 27.4 and 45 µg/100 mL). Relative to usual diet, we calculated change in iodine intake, and the proportion with intake below the Lower Reference Nutrient Intake (LRNI) or above the upper limit.

Results

For all groups, replacement with PBMA, either unfortified or fortified at the lowest concentration, resulted in a meaningful decrease in iodine intake, and increased the proportion with intake < LRNI; compared to usual diet, iodine intake reduced by 58% in children 1.5–3 years (127 vs. 53 µg/day) and the proportion with intake < LRNI increased in girls (11–18 years; 20% to 48%) and WRA (13% to 33%) if an unfortified PBMA was used. Replacement of milk with PBMA fortified at 27.4 µg/100 mL had the lowest impact.

Conclusion

Replacing milk with commercially available PBMAs has potential to reduce population iodine intake, depending on the fortification level. PBMAs fortified with ≥ 22.5 and < 45 µg iodine/100 mL would be required to minimize the impact on iodine intake. Research is needed on the impact of total dairy replacement.
Development and validation of the screening tool for age-related hearing loss in the community based on the information platform
Authors: Ge Jianli , Yan Yunyun , Zhu Yinqian , Cheng Xin , Li Huazhang , Sun Xiaoming , Jiang Hua
Published: January 2024

Introduction

Currently, age-related hearing loss has become prevalent, awareness and screening rates remain dismally low. Duing to several barriers, as time, personnel training and equipment costs, available hearing screening tools do not adequately meet the need for large-scale hearing detection in community-dwelling older adults. Therefore, an accurate, convenient, and inexpensive hearing screening tool is needed to detect hearing loss, intervene early and reduce the negative consequences and burden of untreated hearing loss on individuals, families and society.

Objectives

The study harnessed "medical big data" and "intelligent medical management" to develop a multi-dimensional screening tool of age-related hearing loss based on WeChat platform.

Methods

The assessment of risk factors was carried out by cross-sectional survey, logistic regression model and receiver operating characteristic (ROC) curve analysis. Combining risk factor assessment, Hearing handicap inventory for the elderly screening version and analog audiometry, the screening software was been developed by JavaScript language and been evaluated and verified.

Results

A total of 401 older adults were included in the cross-sectional study. Logistic regression model (univariate, multivariate) and reference to literature mention rate of risk factors, 18 variables (male, overweight/obesity, living alone, widowed/divorced, history of noise, family history of deafness, non-light diet, no exercising habit, smoking, drinking, headset wearer habit, hypertension, diabetes, hyperlipidemia, cardiovascular and cerebrovascular diseases, hyperuricemia, hypothyroidism, history of ototoxic drug use) were defined as risk factors. The area under the ROC curve (AUC) of the cumulative score of risk factors for early prediction of age-related hearing loss was 0.777 [95% CI (0.721, 0.833)]. The cumulative score threshold of risk factors was defined as 4, to classify the older adults into low-risk (< 4) and high-risk (≥ 4) hearing loss groups. The sensitivity, specificity, positive predictive value, and negative predictive value of the screen tool were 100%, 65.5%, 71.8%, and 100.0%, respectively. The Kappa index was 0.6.

Conclusions

The screening software enabled the closed loop management of real-time data transmission, early warning, management, whole process supervision of the hearing loss and improve self-health belief in it. The software has huge prospects for application as a screening approach for age-related hearing loss.
Immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane
Authors: Martín Julio Cesar Molina , Sánchez Laura Fernández , Piñero David P. , Navarro Nicolás Cuenca
Published: January 2024

Purpose

The main purpose of this study was to perform an immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane (ERM).

Methods

Twenty-four specimens of idiopathic ERM from 24 consecutive patients who underwent 23 G pars plana vitrectomy for ERM and internal limiting membrane (ILM) peeling at the San Juan University Hospital in Alicante (Spain) in 2019 were analyzed. All patients underwent a complete ophthalmological examination including measurement of best corrected visual acuity (BCVA) and macular analysis by spectral-domain optical coherence tomography (SD-OCT) at the time of diagnosis and 3 months after surgery. Specific glial fibrillar acid protein antibodies (GFAP) and S100 calcium-binding protein β (S100β) immunostaining markers were used to identify the macroglial component of the ERM, Müller cells, and astrocytes. Ionized calcium-binding adapter molecule 1 protein (Iba1) antibodies were used as specific markers for inflammatory cells, such as microglia and macrophages.

Results

Mean preoperative BCVA measured with Snellen chart was 0.3 and 0.6 preoperatively and at 3 months after surgery, respectively. SD-OCT identified 15 patients (62.5%) with a disruption of the outer retinal hyperreflective bands. The immunohistochemical study showed the presence of Müller cells in almost all cases (91.6%), as well of abundant microglia and macrophages. Microglia and macrophages were more frequently present in earlier stages of ERM. Microglia were present in ERM independently of the outer retinal hyperreflective bands integrity as measured by SD-OCT. A greater presence of macrophages was found in those ERMs with no outer retinal hyperreflective band disruption.

Conclusions

Müller cells seem to be the most frequent cell group in ERMs, with also presence of microglia cells and macrophages. Astrocytes were more frequently found in early stages of ERMs. Microglia and macrophages were most frequent in ERMs with early stage (1, 2, or 3) than in advanced stages (4).
Analysis of laboratory parameters before the occurrence of hepatic sinusoidal obstruction syndrome in children, adolescents, and young adults after hematopoietic stem cell transplantation
Authors: Johann Lorena , Gruhn Bernd
Published: January 2024

Purpose

Hepatic sinusoidal obstruction syndrome (SOS) is a serious complication following hematopoietic stem cell transplantation (HSCT) in which early diagnosis improves patient outcome. The aim of our study was to detect laboratory parameters following HSCT that can predict the occurrence of SOS.

Methods

This retrospective study included 182 children, adolescents, and young adults who underwent allogeneic or autologous HSCT for the first time (median age 7.2 years). The diagnosis of SOS was based on the pediatric criteria of European Society for Blood and Marrow Transplantation (EBMT). We investigated 15 laboratory parameters after HSCT before the onset of SOS.

Results

The overall incidence of SOS was 14.8%. SOS developed in 24 of 126 allogeneic (19.1%) and in 3 of 56 autologous (5.4%) HSCT patients at a median time of 13 days after HSCT. We observed a low SOS mortality rate of 11.1% within 100 days after HSCT. International normalized ratio (INR) ≥ 1.3, activated partial thromboplastin time (aPTT) ≥ 40 s, reptilase time ≥ 18.3 s, factor VIII ≤ 80%, antithrombin III ≤ 75%, protein C ≤ 48%, D-dimer ≥ 315 µg/L, bilirubin ≥ 9 µmol/L, and ferritin ≥ 3100 µg/L showed significant associations with the onset of SOS in the univariate analyses. In the multivariate analysis, INR ≥ 1.3 [odds ratio (OR) = 8.104, p = 0.006], aPTT ≥ 40 s (OR = 10.174, p = 0.001), protein C ≤ 48% (OR = 5.215, p = 0.014), and ferritin ≥ 3100 µg/L (OR = 7.472, p = 0.004) could be confirmed as independent risk factors after HSCT before SOS. If three of the four significant cut-off values were present, the probability of developing SOS was more than 70%. The probability of SOS was 96%, if all four laboratory parameters were changed according to the cut-off values. The values of factor XIII, von Willebrand factor (vWF), von Willebrand factor activity (vWF activity), protein S, fibrinogen, and alanine aminotransferase (ALT) were not relevant for the occurrence of SOS.

Conclusion

In summary, the laboratory parameters INR, aPTT, protein C, and ferritin were very useful to predict the occurrence of SOS. In addition, this is the first report on a significant association between SOS and high values of INR and aPTT after HSCT before SOS.
Predecessor on the Ultra-Wide Word RAM
Authors: Bille Philip , Gørtz Inge Li , Stordalen Tord
Published: January 2024

We consider the predecessor problem on the ultra-wide word RAM model of computation, which extends the word RAM model with ultrawords consisting of $$w^2$$

w
2

bits (TAMC, 2015). The model supports arithmetic and boolean operations on ultrawords, in addition to scattered memory operations that access or modify w (potentially non-contiguous) memory addresses simultaneously. The ultra-wide word RAM model captures (and idealizes) modern vector processor architectures. Our main result is a simple, linear space data structure that supports predecessor in constant time and updates in amortized, expected constant time. This improves the space of the previous constant time solution that uses space in the order of the size of the universe. Our result holds even in a weaker model where ultrawords consist of $$w^{1+\epsilon }$$

w

1
+
ϵ

bits for any $$\epsilon > 0 $$

ϵ
>
0

. It is based on a new implementation of the classic x-fast trie data structure of Willard (Inform Process Lett 17(2):81–84, https://doi.org/10.1016/0020-0190(83)90075-3, 1983) combined with a new dictionary data structure that supports fast parallel lookups.
Analysis of risk factors for acute kidney injury in children with severe wasp stings
Authors: Lu Jing , Dong Liqun , Zhang Lijuan , Guo Yannan , Liu Hanmin , Liu Yang
Published: January 2024

Background

Acute kidney injury (AKI) is common in children with sepsis, chronic kidney disease, poisoning or other conditions. Wasp stings are recognized as an important etiology. Several retrospective studies have investigated AKI after wasp stings in adults, but research on children remains limited.

Methods

The study included 48 children with multiple organ dysfunction syndrome after wasp stings. Demographic data, clinical manifestations, laboratory findings, management and clinical outcomes were collected, and analyzed to identify early indicators or risk factors for AKI.

Results

20 children (41.7%) developed AKI, and 28 (58.3%) did not. Serum creatine levels elevated mostly within 24 h from stings in children with AKI (16/20, 80%). Compared with non-AKI group, AKI group exhibited more cases with cola-colored urine, jaundice, and had higher sting numbers/body surface area (BSA) and higher revised sequential organ failure assessment scores (rSOFA) as well as higher levels of C-reactive protein (CRP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), lactate dehydrogenase (LDH), troponin (cTnI), creatine kinase (CK), and longer prothrombin time (PT). Both univariable and multivariable logistic regression analysis identified cola-colored urine as a potential early risk factor for AKI.

Conclusions

The AKI group exhibited higher sting numbers/BSA, higher levels of CRP, ALT, AST, TBIL, LDH, cTnI, and CK, as well as longer PT (p < 0.05). Our findings also suggest that cola-colored urine may serve as an early indicator or potential risk factor for AKI after wasp stings in children, which is very easy to identify for first aiders or pediatricians.

Graphical abstract
First principle study of ATiO$$_3$$ (A=Ti,Sr) materials for photovoltaic applications
Authors: Allan Lynet , Mulwa Winfred M. , Mapasha R. E. , Mwabora Julius M. , Musembi Robinson J.
Published: January 2024

Context

The study investigates the impact of Hubbard U correction and spin-orbit coupling (SOC) on the structural, mechanical, electronic, and optical properties of $$\text {Ti}_{2}\textrm{O}_{3}$$

Ti
2

O
3

and $$\text {SrTiO}_{3}$$

SrTiO
3

compounds. The research is motivated by the potential applications of these materials in photovoltaics, with a focus on understanding their properties for such use. The ductility, ionicity, and mechanical stability of both compounds at zero pressure are assessed, indicating their potential as resilient materials. Also, the compounds display high refractive indices and absorption coefficients, indicating their suitability for solar harvesting applications. The predicted bandgaps align primarily with the UV–Vis areas of the electromagnetic spectrum, highlighting their potential in this domain.

Methods

Computational techniques employed in this study are density functional theory (DFT) with and without spin-orbit coupling, as well as DFT+U methods, implemented using the Quantum ESPRESSO (QE) package. The study adopts the Perdew-Burke-Ernzerhof (PBE) exchange-correlation functional, while employing a plane-wave basis set with an energy cutoff of 50 Ry for wavefunctions and 500 Ry for charge density.
Analytic regularity and solution approximation for a semilinear elliptic partial differential equation in a polygon
Authors: He Yanchen , Schwab Christoph
Published: January 2024

In an open, bounded Lipschitz polygon $$\Omega \subset \mathbb {R}^2$$

Ω
⊂

R

2

, we establish weighted analytic regularity for a semilinear, elliptic PDE with analytic nonlinearity and subject to a source term f which is analytic in $$\Omega $$
Ω
. The boundary conditions on each edge of $$\partial \Omega $$

∂
Ω

are either homogeneous Dirichlet or homogeneous Neumann BCs. The presently established weighted analytic regularity of solutions implies exponential convergence of various approximation schemes: hp-finite elements, reduced order models via Kolmogorov n-widths of solution sets in $$H^1(\Omega )$$

H
1

(
Ω
)

, quantized tensor formats and certain deep neural networks.
ETV7 promotes colorectal cancer progression through upregulation of IFIT3
Authors: Chai Bao , Li Yanjun , Guo Yarong , Zhang Zhuowei , Jia Kai , Chai Xinhao , Suo Yuhong
Published: January 2024

Members of the E26 transformation-specific (ETS) variant transcription factor family act as either tumor suppressors or oncogenic factors in numerous types of cancer. ETS variant transcription factor 7 (ETV7) participates in the development of malignant tumors, whereas its involvement in colorectal cancer (CRC) is less clear. In this study, The Cancer Genome Atlas (TCGA) and immunochemistry staining were applied to check the clinical relevance of ETV7 and interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) in CRC patients. Overexpression and knockdown of ETV7 and IFIT3 were conducted by transfecting the cells with pCDNA3.1 plasmids and siRNAs, respectively. Western blotting was used to detect the protein expression of ETV7 in CRC cells. Cell Counting Kit-8, cell colony formation, and Transwell assays, as well as flow cytometry, were used to evaluate the proliferation, migration, cell cycle, and apoptosis of CRC cells. Furthermore, western blotting, RT-qPCR, and luciferase assay were used to explore the regulation of ETV7 on IFIT3. Rescue assay was used to investigate the significance of ETV7/IFIT3 axis on CRC progression. We found that ETV7 was upregulated in CRC tissues and cells. Overexpression of ETV7 stimulated the proliferation, migration, and cell cycle amplification, and reduced the apoptosis of CRC cells. Downregulation of ETV7 exerted the opposite effect on CRC cell progression. Moreover, we demonstrated that ETV7 stimulated the transcription activity, the mRNA and protein expression of IFIT3 in CRC cells. There was a positive correlation between ETV7 and IFIT3 in CRC patients. IFIT3 knockdown reversed the promotive effect exerted by overexpression of ETV7 on the amplification and migration of CRC cells. By contrast, overexpression of IFIT3 blocked the inhibitory effect of ETV7-targeting siRNA. In summary, ETV7 induces progression of CRC by activating the transcriptional expression of IFIT3. The EVT7/IFIT3 axis may be a novel target for CRC therapy.
Screening for precancerous anal lesions linked to human papillomaviruses: French recommendations for clinical practice
Authors: Spindler L. , Etienney I. , Abramowitz L. , de Parades V. , Pigot F. , Siproudhis L. , Adam J. , Balzano V. , Bouchard D. , Bouta N. , Bucau M. , Carlo A. , Chanal J. , Charpentier C. , Clifford G. , Draullette M. , Fathallah N. , Ferré V. , Fléjou J.-F. , Fouéré S. , Higuero T. , Kassouri L. , Kurt S. , Laurain A. , Leclerc E. , Lepiller Q. , Lesage A.-C. , Mège D. , Ménard A. , Merle P. , Mortreux P. , Noël C. , Péré H. , Prétet J.-L. , Roland D. , Staumont G. , Tracanelli L. , Vuitton L. , Wylomanski S. , Zaegel-Faucher O. ,   Société Nationale Française de Colo-Proctologie
Published: January 2024

In France, about 2000 new cases of anal cancer are diagnosed annually. Squamous cell carcinoma is the most common histological type, mostly occurring secondary to persistent HPV16 infection. Invasive cancer is preceded by precancerous lesions. In addition to patients with a personal history of precancerous lesions and anal cancer, three groups are at very high risk of anal cancer: (i) men who have sex with men and are living with HIV, (ii) women with a history of high-grade squamous intraepithelial lesions (HSILs) or vulvar HPV cancer, and (iii) women who received a solid organ transplant more than 10 years ago. The purpose of screening is to detect HSILs so that they can be treated, thereby reducing the risk of progression to cancer. All patients with symptoms should undergo a proctological examination including standard anoscopy. For asymptomatic patients at risk, an initial HPV16 test makes it possible to target patients at risk of HSILs likely to progress to cancer. Anal cytology is a sensitive test for HSIL detection. Its sensitivity is greater than 80% and exceeds that of proctological examination with standard anoscopy. It is indicated in the event of a positive HPV16 test. In the presence of cytological abnormalities and/or lesions and a suspicion of dysplasia on clinical examination, high-resolution anoscopy is indicated. Performance is superior to that of proctological examination with standard anoscopy. However, this technique is not widely available, which limits its use. If high-resolution anoscopy is not possible, screening by a standard proctological examination is an alternative. There is a need to develop high-resolution anoscopy and triage tests and to evaluate screening strategies.
A systematic literature review on business-IT misalignment research
Authors: Őri Dóra , Szabó Zoltán
Published: January 2024

There has been a large body of research on strategic alignment between business and information technology, which has also been summarised in several literature reviews. All of these studies describe that business-IT alignment has remained a focal point among business and IT leaders. However, little is known about a specific perspective, namely, on business-IT misalignment, on which, although some analytical works have been carried out over the last twenty years, no literature review has been summarised. The purpose of this article is to display and analyze relevant literature regarding business-IT misalignment and map the influential issues by conducting a systematic literature review. This study collected in sum 642 papers published from the Scopus and Google Scholar databases. Finally, 62 articles were selected for the systematic review. The study examined eight research questions for business-IT misalignment derived from recent, high-impact business-IT alignment literature reviews. Results are analyzed qualitatively to find a better understanding of the current body of knowledge in business-IT misalignment and to provide a research agenda.
CT-Based Intratumoral and Peritumoral Radiomics Nomograms for the Preoperative Prediction of Spread Through Air Spaces in Clinical Stage IA Non-small Cell Lung Cancer
Authors: Wang Yun , Lyu Deng , Hu Lei , Wu Junhong , Duan Shaofeng , Zhou Taohu , Tu Wenting , Xiao Yi , Fan Li , Liu Shiyuan
Published: January 2024

The study aims to investigate the value of intratumoral and peritumoral radiomics and clinical-radiological features for predicting spread through air spaces (STAS) in patients with clinical stage IA non-small cell lung cancer (NSCLC). A total of 336 NSCLC patients from our hospital were randomly divided into the training cohort (n = 236) and the internal validation cohort (n = 100) at a ratio of 7:3, and 69 patients from the other two external hospitals were collected as the external validation cohort. Univariate and multivariate analyses were used to select clinical-radiological features and construct a clinical model. The GTV, PTV5, PTV10, PTV15, PTV20, GPTV5, GPTV10, GPTV15, and GPTV20 models were constructed based on intratumoral and peritumoral (5 mm, 10 mm, 15 mm, 20 mm) radiomics features. Additionally, the radscore of the optimal radiomics model and clinical-radiological predictors were used to construct a combined model and plot a nomogram. Lastly, the ROC curve and AUC value were used to evaluate the diagnostic performance of the model. Tumor density type (OR = 6.738) and distal ribbon sign (OR = 5.141) were independent risk factors for the occurrence of STAS. The GPTV10 model outperformed the other radiomics models, and its AUC values were 0.887, 0.876, and 0.868 in the three cohorts. The AUC values of the combined model constructed based on GPTV10 radscore and clinical-radiological predictors were 0.901, 0.875, and 0.878. DeLong test results revealed that the combined model was superior to the clinical model in the three cohorts. The nomogram based on GPTV10 radscore and clinical-radiological features exhibited high predictive efficiency for STAS status in NSCLC.
Robustness of Deep Networks for Mammography: Replication Across Public Datasets
Authors: Velarde Osvaldo M. , Lin Clarissa , Eskreis-Winkler Sarah , Parra Lucas C.
Published: January 2024

Deep neural networks have demonstrated promising performance in screening mammography with recent studies reporting performance at or above the level of trained radiologists on internal datasets. However, it remains unclear whether the performance of these trained models is robust and replicates across external datasets. In this study, we evaluate four state-of-the-art publicly available models using four publicly available mammography datasets (CBIS-DDSM, INbreast, CMMD, OMI-DB). Where test data was available, published results were replicated. The best-performing model, which achieved an area under the ROC curve (AUC) of 0.88 on internal data from NYU, achieved here an AUC of 0.9 on the external CMMD dataset (N = 826 exams). On the larger OMI-DB dataset (N = 11,440 exams), it achieved an AUC of 0.84 but did not match the performance of individual radiologists (at a specificity of 0.92, the sensitivity was 0.97 for the radiologist and 0.53 for the network for a 1-year follow-up). The network showed higher performance for in situ cancers, as opposed to invasive cancers. Among invasive cancers, it was relatively weaker at identifying asymmetries and was relatively stronger at identifying masses. The three other trained models that we evaluated all performed poorly on external datasets. Independent validation of trained models is an essential step to ensure safe and reliable use. Future progress in AI for mammography may depend on a concerted effort to make larger datasets publicly available that span multiple clinical sites.
CTG18.1 expansion in transcription factor 4 (TCF4) in corneal graft failure: preliminary study
Authors: Westin Ida Maria , Viberg Andreas , Golovleva Irina , Byström Berit
Published: January 2024

Fuchs endothelial corneal dystrophy (FECD) is caused by a corneal endothelial cell loss, leading to corneal edema and visual impairment. The most significant genetic risk factor for FECD is an expansion of the CTG18.1 locus in transcription factor 4 (TCF4). The current treatment for severe FECD is corneal transplantation, with Descemet stripping automated keratoplasty (DSAEK) as a common surgical method. Although successful in most cases, the risk for transplant failure due to diverse causes must be considered. In this study, we investigated if presence of TCF4 CTG18.1 expansion with more than 31 (n ≥ 31) repeats in donated corneal grafts could be a reason for corneal transplant failure after DSAEK. For this, nine consecutively failed DSAEK corneal grafts were genotyped for CTG18.1 repeat length. One-sided Mann–Whitney U test was performed to evaluate if failed DSAEK corneal grafts had longer CTG18.1 repeats than healthy controls from the same population. All failed corneal grafts had CTG18.1 n ≤ 27 with a median of 18 (IQR 8.0) repeats for the longest allele. There was no statistical difference in CTG18.1 repeat lengths between failed corneal grafts and the geographically matched healthy control group. In conclusion, none of the nine failed corneal grafts in our material had CTG18.1 repeat lengths ≥ 31, a cut-off known to have a biological relevance in FECD. Thus, our results suggest that the assessment of donors and inspection of the corneal tissue before the decision for procurement is sufficient, in terms of recognizing FECD in the donor.
Phenotypic diversity analysis of Polygala tenuifolia Willd germplasm resources in China
Authors: Tian Hongling , Wu Changjuan , Zhang Lijun , Guo Shuhong , Wang Yaoqin , Pei Shuaishuai , Zuo Xianqiang , Wang Qiubao , Zhang Qiang
Published: January 2024

Polygala tenuifolia Willd is a perennial herb in the family Polygalaceae and is used as an expectorant and stimulant in traditional Chinese medicine. There is a large demand for P. tenuifolia, while the good cultivars of P. tenuifolia are not enough. In this study, we collected 157 germplasm resources of P. tenuifolia from different regions in China, and 12 phenotypic traits of these samples were analyzed using correlation analysis, principal component analysis (PCA), and cluster analysis methods. The correlation analysis showed the associations between different traits, and the first four principal components contributed 62.63% of the total variation. They contained most of the information of five above-ground traits and eight root traits. Phylogenetic clustering divided materials divided into four taxa, with characteristics of high-yielding specific material, long-rooted specific material, multi-branching specific material, and high plant height specific material, respectively. Our study provides the material and theoretical basis for the utilization of germplasm resources, parental selection, and varietal improvement of P. tenuifolia.
He Taonga Te Wareware: Connecting Older Māori Experiences of Wairuatanga with Mate Wareware (Dementia)
Authors: Te Maringi Mai o Hawaiiki  , Menzies Oliver , Garrett Nick , Dudley Makarena
Published: January 2024

Mate wareware (dementia) is a complex disease of the brain that progressively inhibits memory and cognitive ability, affecting many Māori (the Indigenous people of Aotearoa New Zealand) kaumātua (elderly persons) in Aotearoa (New Zealand). Mate wareware care aims to protect and sustain wellbeing, yet Māori perspectives of wellbeing that consider wairuatanga (Māori spirituality) are often neglected within current treatment planning. This study investigates the presence of wairuatanga within kaumātua lives, drawing upon 61 interviews with kaumātua to glean a Māori understanding of mate wareware and to develop a diagnostic screening tool for mate wareware. Recorded responses were thematically analysed using reflexive qualitative analysis, informing four key themes that influence wairuatanga: he hononga tangata (social connection), tūrangawaewae (places of connection), tuakiritanga (identity) and mahi mauritau (mindful practices). These themes consider the value of creating rich and gratifying lifestyles for kaumātua that cultivate their spiritual wellbeing. This study validates diverse understandings and experiences of wairuatanga as essential to Māori wellbeing, affirming the relevance of wairuatanga to improve outcomes for Māori living with mate wareware.
A proposed index of myocardial staining for vein of Marshall ethanol infusion: an Italian single-center experience
Authors: Landra Federico , Nesti Martina , Garibaldi Silvia , Mirizzi Gianluca , Startari Umberto , Panchetti Luca , Piacenti Marcello , Taddeucci Simone , Formichi Bruno Antonio , Stefani Maurizio , Galiberti Serena , Lionetti Vincenzo , Solinas Paolo , Levantesi Beatrice Maria , Italia Chiara , Rossi Andrea
Published: January 2024

Background

Mitral isthmus (MI) conduction block is a fundamental step in anatomical approach treatment for persistent atrial fibrillation (PeAF). However, MI block is hardly achievable with endocardial ablation only. Retrograde ethanol infusion (EI) into the vein of Marshall (VOM) facilitates MI block. Fluorographic myocardial staining (MS) during VOM-EI could be helpful in predicting procedural alcoholization outcome even if its role is qualitatively assessed in the routine. The aim was to quantitatively assess MS during VOM-EI and to evaluate its association with MI block achievement.

Methods

Consecutive patients undergoing catheter ablation for PeAF at Fondazione Toscana Gabriele Monasterio (Pisa, Italy) from February 2022 to May 2023 were considered. Patients with identifiable VOM were included. A proposed index of MS (MSI) was retrospectively calculated in each included patient. Correlation of MSI with low-voltage zones (LVZ) extension after VOM-EI and its association with MI block achievement were assessed.

Results

In total, 42 patients out of 49 (85.8%) had an identifiable VOM. MI block was successfully achieved in 35 patients out of 42 (83.3%). MSI was significantly associated with the occurrence of MI block (OR 1.24 (1.03–1.48); p = 0.022). A higher MSI resulted in reduced ablation time (p = 0.014) and reduced radiofrequency applications (p = 0.002) to obtain MI block. MSI was also associated with MI block obtained by endocardial ablation only (OR 1.07 (1.02–1.13); p = 0.002). MSI was highly correlated with newly formed LVZ extension (r = 0.776; p = 0.001).

Conclusions

In our study cohort, optimal MSI predicts MI block and facilitates its achievement with endocardial ablation only.
Surface characteristics and in vitro biocompatibility of titanium preserved in a vitamin C-containing saline storage solution
Authors: Zhang Wen-si , Liu Yao , Shao Shui-Yi , Shu Chang-qing , Zhou Yi-heng , Zhang Song-mei , Qiu Jing
Published: January 2024

The purpose of this study is to explore a storage solution for titanium implants and investigate its osteogenic properties. The commercial pure titanium (cp-Ti) surface and double-etched (SLA) titanium surface specimens were preserved in air, saline, 10 mM Vitamin C (VitC)-containing saline and 100 mM VitC-containing saline storage solutions for 2 weeks. The surface microtopography of titanium was observed by scanning electron microscopy (SEM), the surface elemental compositions of the specimens were analyzed by Raman and X-ray photoelectron spectroscopy (XPS), and water contact angle and surface roughness of the specimens were tested. The protein adsorption capacity of two titanium surfaces after storage in different media was examined by BCA kit. The MC3T3-E1 osteoblasts were cultured on two titanium surfaces after storage in different media, and the proliferation, adhesion and osteogenic differentiation activity of osteoblasts were detected by CCK-8, laser confocal microscope (CLSM) and Western blot. The SEM results indicated that the titanium surfaces of the air group were relatively clean while scattered sodium chloride or VitC crystals were seen on the titanium surfaces of the other three groups. There were no significant differences in the micromorphology of the titanium surfaces among the four groups. Raman spectroscopy detected VitC crystals on the titanium surfaces of two experimental groups. The XPS, water contact angle and surface roughness results suggested that cp-Ti and SLA-Ti stored in 0.9% NaCl and two VitC-containing saline storage solutions possessed less carbon contamination and higher surface hydrophilicity. Moreover, the protein adsorption potentials of cp-Ti and SLA-Ti surfaces were significantly improved under preservation in two VitC-containing saline storage solutions. The results of in vitro study showed that the preservation of two titanium surfaces in 100 mM VitC-containing saline storage solution upregulated the cell adhesion, proliferation, osteogenic related protein expressions of MC3T3-E1 osteoblasts. In conclusion, preservation of cp-Ti and SLA-Ti in 100 mM VitC-containing saline storage solution could effectively reduce carbon contamination and enhance surface hydrophilicity, which was conducive to osteogenic differentiation of osteoblasts.

Graphical Abstract
A Bayesian Aoristic Logistic Regression to Model Spatio-Temporal Crime Risk Under the Presence of Interval-Censored Event Times
Authors: Briz-Redón Álvaro
Published: January 2024

Purpose

Crime data analysis has gained significant interest due to its peculiarities. One key characteristic of property crimes is the uncertainty surrounding their exact temporal location, often limited to a time window.

Methods

This study introduces a spatio-temporal logistic regression model that addresses the challenges posed by temporal uncertainty in crime data analysis. Inspired by the aoristic method, our Bayesian approach allows for the inclusion of temporal uncertainty in the model.

Results

To demonstrate the effectiveness of our proposed model, we apply it to both simulated datasets and a dataset of residential burglaries recorded in Valencia, Spain. We compare our proposal with a complete cases model, which excludes temporally-uncertain events, and also with alternative models that rely on imputation procedures. Our model exhibits superior performance in terms of recovering the true underlying crime risk.

Conclusions

The proposed modeling framework effectively handles interval-censored temporal observations while incorporating covariate and space–time effects. This flexible model can be implemented to analyze crime data with uncertainty in temporal locations, providing valuable insights for crime prevention and law enforcement strategies.