bluuebunny/crossref_metadata_2025 huggingface dataset:
The Persistence of Hepatitis C Virus Infection in Hepatocytes Promotes Hepatocellular Carcinoma Progression by Pro-Inflammatory Interluekin-8 Expression
Authors: Shabangu Ciniso Sylvester , Siphepho Phumelele Yvonne , Li Chia-Yang , Cheng Wei-Chung , Lu Ming-Ying , Huang Chung-Feng , Yeh Ming-Lun , Dai Chia-Yen , Huang Jee-Fu , Chuang Wan-Long , Lin Zu-Yau , Yu Ming-Lung , Wang Shu-Chi
Published: October 2021

Background: A large amount of epidemiological evidence indicates that persistent HCV infection is the main risk factor for HCC. We aimed to study the effects of persistent HCV infection on the interaction of the virus and host cell to identify cancer gene profiles. Methods: Next-generation sequencing (NGS) was used to identify differentially expressed genes between uninfected Huh7.5.1 control cells, short-term HCV (S-HCV), early long-term HCV (eL-HCV), and long-term HCV (L-HCV) infections, which were analyzed using different dynamic bioinformatics and analytic tools. mRNA expression was validated and quantified using q-PCR. One hundred ninety-six serum samples of HCV patients with IFN/RBV treatment were used to study chemokine levels. Results: S-HCV activates an inflammatory response and drives cell death and apoptosis through cell cycle arrest via MAPK signaling. L-HCV promotes cell growth and alters cell adhesion and chemokine signaling via CXCL8-mediated-SRC regulation. A total of 196 serum samples from the HCV and HCV-HCC cohorts demonstrated significantly upregulated pro-inflammatory CXCL8 in non-SVR (persistent HCV infection) patients in the HCV-HCC group. Conclusions: Persistent infection with HCV induced pro-inflammatory CXCL8 and the oncogene SRC, thereby triggering and promoting hepatocarcinogenesis. CXCL8 may be a potential biomarker for monitoring HCV-related HCC progression.
Emerging Landscape of Immunotherapy for Primary Central Nervous System Lymphoma
Authors: Alcantara Marion , Fuentealba Jaime , Soussain Carole
Published: October 2021

Primary central nervous system lymphoma (PCNSL) is, mainly, a diffuse large B-cell lymphoma (DLBCL) with a non-germinal center B-cell (non-GCB) origin. It is associated with a poor prognosis and an unmet medical need. Immunotherapy has emerged as one of the most promising areas of research and is now part of the standard treatment for many solid and hematologic tumors. This new class of therapy generated great enthusiasm for the treatment of relapsed/refractory PCNSL. Here, we discuss the challenges of immunotherapy for PCNSL represented by the lymphoma cell itself and the specific immune brain microenvironment. We review the current clinical development from the anti-CD20 monoclonal antibody to CAR-T cells, as well as immune checkpoint inhibitors and targeted therapies with off-tumor effects on the brain microenvironment. Perspectives for improving the efficacy of immunotherapies and optimizing their therapeutic role in PCNSL are suggested.
Magnetic Guiding with Permanent Magnets: Concept, Realization and Applications to Nanoparticles and Cells
Authors: Blümler Peter
Published: October 2021

The idea of remote magnetic guiding is developed from the underlying physics of a concept that allows for bijective force generation over the inner volume of magnet systems. This concept can equally be implemented by electro- or permanent magnets. Here, permanent magnets are in the focus because they offer many advantages. The equations of magnetic fields and forces as well as velocities are derived in detail and physical limits are discussed. The special hydrodynamics of nanoparticle dispersions under these circumstances is reviewed and related to technical constraints. The possibility of 3D guiding and magnetic imaging techniques are discussed. Finally, the first results in guiding macroscopic objects, superparamagnetic nanoparticles, and cells with incorporated nanoparticles are presented. The constructed magnet systems allow for orientation, movement, and acceleration of magnetic objects and, in principle, can be scaled up to human size.
The Relevance of the Adverse Childhood Experience International Questionnaire to Working Children: Knowledge Gaps and Implications for Policy Makers
Authors: Rutter Alice
Published: October 2021

(1) Adverse childhood experiences (ACE) are a global challenge, prioritized in the United Nations’ Sustainable Development Goals. The ACE questionnaire is widely adopted in the USA as a tool for measuring population-level trends, such as negative health, behavioral, and economic outcomes. Intuitively, children in resource-scarce settings are exposed to higher levels of trauma. To understand the global picture, the World Health Organization (WHO) adapted the ACE international questionnaire (ACE-IQ), to inform policy and target interventions. However, evaluation of whether the ACE-IQ captures the experiences of around 160 million working children remains limited. (2) I applied the ACE-IQ scoring tools to detailed case studies of working children, comparing issues highlighted by holistic assessment to those captured by the ACE-IQ. (3) The ACE-IQ struggles to capture nuance across cultural contexts. As a consequence, application of the ACE-IQ as a policy tool risks “policy failure”. The tool reflects prevalent Western concerns, such as school attendance and parental supervision, but global concerns affecting working children such as forced economic migration and famine are neglected. This limitation produces “policy myopia”, sidelining certain global challenges. (4) The ACE-IQ is a useful public health tool, increasingly used to define policy goals. However, given the limitations of the ACE-IQ, the consequences of prioritizing these particular policy goals need to be actively acknowledged and mitigated.
Innovative Techniques Associated with Traditional Abdominal Surgery in Complex Pediatric Cases: A Tertiary Center Experience
Authors: Pulvirenti Rebecca , Tognon Costanza , Bisoffi Silvia , Ghidini Filippo , De Corti Federica , Fascetti Leon Francesco , Antoniello Luca Maria , Gamba Piergiorgio
Published: October 2021

Pediatric abdominal surgery is constantly evolving, alongside the advent of new surgical technologies. A combined use of new tools and traditional surgical approaches can be useful in the management of complex cases, allowing less invasive procedures and sometimes even avoiding multiple interventions. This combination of techniques has implications even from the anesthetic point of view, especially in post-operative pain control. Thereby, tertiary level centres, including highly-specialized professionals and advanced equipment, can maximize the effectiveness of treatments to improve the final outcomes. Our paper aims to present some possible combinations of techniques recently used at our institution to provide a one-session, minimally invasive treatment within different areas of abdominal surgery.
Management of Severely Aberrant Permanent First Molars in Molar Root–Incisor Malformation Patients: Case Series and a Guideline
Authors: Song Ji-Soo , Yang Yeon-Mi , Kim Young-Jae , Kim Jung-Wook
Published: October 2021

Recently, a new type of dental anomaly, a molar–incisor malformation or molar root–incisor malformation (MRIM), was recognized. The disease phenotype is now relatively well characterized; however, its etiology and disease-mechanism need to be elucidated. The affected teeth do not respond well to conventional treatment because of severe malformation and an unusual root structure. In this study, we present the treatment of MRIM cases with the extraction of severely aberrant permanent first molars (PFMs) and suggest that the PFM extractions are performed when it is clear that third molars will develop. The purpose of this report was primarily to present amendments to the guidelines for the treatment of patients with MRIM.
Road Salt Damage to Historical Milestones Indicates Adaptation of Winter Roads to Future Climate Change May Damage Arctic Cultural Heritage
Authors: Antonson Hans , Buckland Philip , Blomqvist Göran
Published: October 2021

There is no doubt that anthropogenic global warming is accelerating damage to cultural heritage. Adaptation measures are required to reduce the loss of sites, monuments and remains. However, little research has been directed towards understanding potential impacts of climate adaptation measures in other governmental sectors on cultural heritage. We provide a case study demonstrating that winter road salt, used to reduce ice related accidents, damages historical iron milestones. As the climate warms, road salt use will move north into areas where sites have been protected by contiguous winter snow cover. This will expose Artic/sub-Arctic cultural heritage, including Viking graves and Sami sites, to a new anthropogenic source of damage. Research and planning should therefore include the evaluation of secondary impacts when choosing climate adaptation strategies.
Developing Physics Experiments Using Augmented Reality Game-Based Learning Approach: A Pilot Study in Primary School
Authors: Zafeiropoulou Maria , Volioti Christina , Keramopoulos Euclid , Sapounidis Theodosios
Published: October 2021

The augmented reality game-based learning (ARGBL) approach is an advantageous pathway for the development and enhancement of teaching and learning processes. To this end, this paper presents the design and development of an ARGBL application for the implementation of physics experiments in the fifth grade of a Greek primary school. The purpose of the ARGBL system is twofold: to educate and entertain. For this reason, a treasure hunt game was implemented, which allows students to interact with a digital world and to manipulate virtual objects with the use of an augmented reality (AR) device. Then, according to the instructions, students have to collect all the materials to conduct the AR educational experiment. Overall, the evaluation of the system’s usability by 17 users (both students and teachers) was very promising, indicating that the ARGBL application has the potential to be an easy-to-use educational tool for improving not only the teaching of physics experiments in primary school but also the learning process, by positively affecting the students’ motivation and engagement.
The Effects of Li+ Doping on Structure and Upconversion Luminescent Properties for Bi3.46Ho0.04Yb0.5Ti3O12: xLi Phosphors
Authors: Ren Feng , Zhou Jinlei , Wang Dengpeng , Wang Xianran , Gao Feng
Published: October 2021

A series of novel Li+ doped Bi3.46Ho0.04Yb0.5Ti3O12 (BHYTO: xLi, 0 ≤ x ≤ 0.15) upconversion phosphors were prepared through a sol-gel-sintering method. There exist three emission bands centered at 545 nm, 658 nm, and 756 nm in the upconversion emission spectra at 980 nm excitation, corresponding to energy transitions of 5F4/5S2 → 5I8, 5F5 → 5I8 and 5F4/5S2 → 5I7 of Ho3+, and the upconversion emission intensity of BHYTO: 0.05Li is about 2.2 times stronger than that of BHYTO samples. The luminescent lifetime of the strongest emission (545 nm) is in the range of 45.25 to 65.99 μs for the different BHYTO: xLi phosphors. The energy transfers during the upconversion pumping process from Yb3+ to Ho3+ are mainly responsible for all the emissions, each belonging to a double-photon process. Li+ mainly entered into the interspace sites or occupied Bi3+ sites in Bi4Ti3O12 host during the fabrication process according to its dosage, and the possibility is very low for Li+ to take part in the energy transfer process directly due to its lack of matching levels with 4f of Ho3+ and Yb3+. However, Li+ doping can not only increase the size of crystal grains to improve crystallinity through XRD analysis, but also reduced oxygen vacancies to decrease the number of quenching centers through XPS analysis. The improved crystallinity and reduced quenching centers are proposed to be the main causes for the enhanced upconversion luminescence of the Li+ doped BHYTO phosphor.
The Retreat of Mountain Glaciers since the Little Ice Age: A Spatially Explicit Database
Authors: Marta Silvio , Azzoni Roberto Sergio , Fugazza Davide , Tielidze Levan , Chand Pritam , Sieron Katrin , Almond Peter , Ambrosini Roberto , Anthelme Fabien , Alviz Gazitúa Pablo , Bhambri Rakesh , Bonin Aurélie , Caccianiga Marco , Cauvy-Fraunié Sophie , Ceballos Lievano Jorge Luis , Clague John , Cochachín Rapre Justiniano Alejo , Dangles Olivier , Deline Philip , Eger Andre , Cruz Encarnación Rolando , Erokhin Sergey , Franzetti Andrea , Gielly Ludovic , Gili Fabrizio , Gobbi Mauro , Guerrieri Alessia , Hågvar Sigmund , Khedim Norine , Kinyanjui Rahab , Messager Erwan , Morales-Martínez Marco Aurelio , Peyre Gwendolyn , Pittino Francesca , Poulenard Jerome , Seppi Roberto , Chand Sharma Milap , Urseitova Nurai , Weissling Blake , Yang Yan , Zaginaev Vitalii , Zimmer Anaïs , Diolaiuti Guglielmina Adele , Rabatel Antoine , Ficetola Gentile Francesco
Published: October 2021

Most of the world’s mountain glaciers have been retreating for more than a century in response to climate change. Glacier retreat is evident on all continents, and the rate of retreat has accelerated during recent decades. Accurate, spatially explicit information on the position of glacier margins over time is useful for analyzing patterns of glacier retreat and measuring reductions in glacier surface area. This information is also essential for evaluating how mountain ecosystems are evolving due to climate warming and the attendant glacier retreat. Here, we present a non-comprehensive spatially explicit dataset showing multiple positions of glacier fronts since the Little Ice Age (LIA) maxima, including many data from the pre-satellite era. The dataset is based on multiple historical archival records including topographical maps; repeated photographs, paintings, and aerial or satellite images with a supplement of geochronology; and own field data. We provide ESRI shapefiles showing 728 past positions of 94 glacier fronts from all continents, except Antarctica, covering the period between the Little Ice Age maxima and the present. On average, the time series span the past 190 years. From 2 to 46 past positions per glacier are depicted (on average: 7.8).
Survey Methods to Identify Risk of Attrition: Measures of Career Intention and Regret
Authors: Carver Mark
Published: October 2021

The common measure of teacher retention as snapshots of those employed in state-funded schools may overestimate attrition by failing to consider a desire for flexibility in contemporary teaching careers. When used as a measure of the effectiveness of teacher education, an over-emphasis on classroom teacher supply may also narrow the curriculum to teacher training rather than the more expansive ‘learning teaching’. This paper discusses two ‘softer’ measures of retention, career intention and training regret, to give a more general sense of how contemporary teachers see their career development as relating to their initial teacher education and professional learning. These measures are generated by adapting survey questions from the OECD’s TALIS and the US’ Beginning Teacher Longitudinal Study, simply asking teachers where they see themselves in five years’ time and if they would still choose to become a teacher if they could go back to before they began training. Surveys were administered annually to two cohorts of recent graduates as part of the Measuring Quality in Initial Teacher Education project—three data captures for 2018 graduates, two for 2019 graduates. It is shown how these measures help to mitigate declines in survey response and can give some helpful estimates of teacher attrition with respect to sex, ethnicity, school type, and degree type. The alternative measures are also argued to give helpful indicators of attrition risk before it happens, allowing discussion of how teachers’ career intentions change during their early careers. In particular, it is found that leaving the classroom is a fairly common expectation, but not necessarily because of teacher burnout. It is suggested that asking what teachers can imagine themselves doing is an effective measure for engaging with issues around vocational choice and teaching as a lifelong profession, with implications for how careers in education are conceptualised in initial teacher education programmes.
Controlling-Supportive Homework Help Partially Explains the Relation between Parents’ Math Anxiety and Children’s Math Achievement
Authors: Retanal Fraulein , Johnston Nichole B. , Di Lonardo Burr Sabrina M. , Storozuk Andie , DiStefano Michela , Maloney Erin A.
Published: October 2021

Previous research has shown that math homework help of higher-math-anxious parents impedes children’s math learning and facilitates the development of math anxiety. In the present study, we explored a possible explanation for this phenomenon by examining the relations between parents’ math anxiety, their math homework-helping styles (i.e., autonomy- and controlling-supportive), and their child’s math achievement. Parents of children ages 11 to 14 completed an online survey. Using path analysis, we examined the relations among parental factors (i.e., math anxiety, math ability, and homework-helping styles) and child math achievement. Parents’ math anxiety was positively related to both autonomy-supportive and controlling-supportive math homework-helping styles. Notably, controlling-supportive style partially mediated the relation between parents’ math anxiety and their children’s math achievement. Thus, it is possible that the use of a controlling-supportive math homework-helping style may explain why the homework help offered by higher-math-anxious parents is detrimental to their children’s math learning. Identifying negative relations between parent factors and children’s math outcomes is crucial for developing evidence-based math learning interventions.
Current Trends in Game-Based Learning—Introduction to a Special Collection of Research
Authors: Marques Margarida M. , Pombo Lúcia
Published: October 2021

The potential of digital games to promote learning is a growing field of research [...]
Implementation of a Playful Microproject Based on Traditional Games for Working on Mathematical and Scientific Content
Authors: Fernández-Oliveras Alicia , Espigares-Gámez María José , Oliveras María Luisa
Published: October 2021

According to previous research, we consider it necessary to extend the use of games, as mediating elements, in the learning of STEAM (Science, Technology, Engineering, Arts and Mathematics) contents rejected by many students. For this, we have carried out an educational research project on games, with an ethnomathematical approach, since games are an important cultural sign with mathematical and scientific potentialities. We have prepared an anthropological study and an analytical one, generating a catalogue of games from different cultures. Thus, we have verified that, starting with culture, we can get to the game, but we posed the query as to whether, starting from certain games, we could achieve enculturation, by activating mathematical and scientific content in the players. To answer this query, we have created a curricular design called “playful microproject” with three traditional games from different cultures and geographical contexts. The microproject was implemented with 32 participants, from 8 to 12 years old. To analyse the results of the microproject, a case study was carried out using qualitative methodology. As part of the playful microproject, the necessary materials for each game were made by hand, and the games were then played. Both the realization of the games and the act of playing showed evidence of mathematical and scientific content, although more in the act of playing. The results revealed that: (1) the three games mobilized 21 categories of analysis, made up of scientific-mathematical content; (2) the three games proved to be equivalent in strong didactic potential; (3) that the microproject provides a valuable intercultural educational approach. The contents evidenced constitute a fundamental part of the Primary Education curriculum: classify, organize, measure, and quantify items, as well as formulate hypotheses, draw conclusions, place oneself in space, and design strategies, among others. It is concluded that these games can promote scientific-mathematical enculturation in a contextualized way.
Parental Play Beliefs in the Developing Areas of China: A Multiple Case Study
Authors: Zhang Yiqi , Leung Suzannie K. Y. , Li Hui
Published: October 2021

Existing studies have explored parental play beliefs in the developed coastal cities in mainland China, leaving parents in developing areas unstudied. This study aimed to understand how these understudied parents view and engage in their children’s play at home, using Bronfenbrenner’s process–person–context–time (PPCT) model. Eight families were interviewed and observed to explore parental beliefs and practices regarding young children’s play at home. Thematic analysis showed that most parents appreciated the importance of play in children’s early development but did not know how to scaffold their children’s play activities. In addition, the high SES families supported child-led play (i.e., free play), whereas the lower SES families adopted traditional rule-based and adult-driven modes. Therefore, more parent education programs and support should be provided to lower SES families in these developing areas.
A WiFi-Based Sensor Network for Flood Irrigation Control in Agriculture
Authors: Lloret Jaime , Sendra Sandra , García-Fernández Julia , García Laura , Jimenez Jose M.
Published: October 2021

The role of agriculture in society is vital due to factors such as providing food for the population, is a major source of employment worldwide, and one of the most important sources of revenue for countries. Furthermore, in recent years, the interest in optimizing the use of water resources has increased due to aspects such as climate change. This has led to the introduction of technology in the fields by means of sensor networks that allow remote monitoring and control of cultivated lands. In this paper, we present a system for flood irrigation in agriculture comprised of a sensor network based on WiFi communication. Different sensors measure atmospheric parameters such as temperature, humidity, and rain, soil parameters such as humidity, and water parameters such as water temperature, salinity, and water height to decide on the need of activating the floodgates for irrigation. The user application displays the data gathered by the sensors, shows a graphical representation of the state of irrigation of each ditch, and allows farmers to manage the irrigation of their fields. Finally, different tests were performed on a plot of vegetables to evaluate the correct performance of the system and the coverage of the sensor network on a vegetated area with different deployment options.
Substrate Integrated Waveguide Antenna System for 5G In-Band Full Duplex Applications
Authors: Shah Masaud , Cheema Hammad M. , Abbasi Qammer H.
Published: October 2021

In-band full duplex offers a new approach of meeting the ever-increasing data rate demands by operating the transmitter and receiver at the same frequency at the same time, potentially doubling the spectral efficiency. However, self-interference is the fundamental bottleneck of such systems. In contrast to non-planar or sub 6 GHz microstrip designs reported so-far, this paper presents an all SIW based antenna system for in-band full duplex systems. The proposed design integrates a dual linear polarized three port differential antenna, three port SIW common-mode power combiner and a 180°phase shifter at 28 GHz. Operating the antenna in TE201 mode provides inherent isolation between the differential receive and single-ended transmit port. The residual coupling is further reduced through use of TE101 based power combiner and a 180°phase shifter. Implemented on a 0.508 mm thick RT Duroid 5880 substrate, the antenna occupies a foot-print of 48 × 80 mm2. Demonstrating a measured gain of 6.95 dBi and 3.42 dBi for Tx and Rx mode of operation, respectively, the proposed design offers a self-interference cancellation (SiC) of better than 36 dB over a 177 MHz bandwidth.
HTNURL: Design of a High-Performance Low-Cost Triple-Node Upset Self-Recoverable Latch
Authors: Xu Hui , Peng Zehua , Liang Huaguo , Huang Zhengfeng , Sun Cong , Zhou Le
Published: October 2021

A high-performance and low power consumption triple-node upset self-recoverable latch (HTNURL) is proposed. It can effectively tolerate single-node upset (SNU), double-node upset (DNU), and triple-node upset (TNU). This latch uses the C-element to construct a feedback loop, which reduces the delay and power consumption by fast path and clock gating techniques. Compared with the TNU-recoverable latches, HTNURL has a lower delay, reduced power consumption, and full self-recoverability. The delay, power consumption, area overhead, and area-power-delay product (APDP) of the HTNURL is reduced by 33.87%, 63.34%, 21.13%, and 81.71% on average.
Using Graphics Processing Units and Compute Shaders in Real Time Multimodel Adaptive Robust Control
Authors: Mihai Cosmin-Constantin , Lupu Ciprian
Published: October 2021

Graphics processing units and video cards have seen a surge of usage in domains other than graphics computers, due to advances in hardware and software technologies; however, little uptake has been in the domain of systems engineering and real time control. This research article will demonstrate the use of video cards in multimodel adaptive robust control, using openGL and compute shaders. A software simulation will show the behavior of the adaptive robust multimodel control scheme as the target process is exposed to both parametric and structural disturbances and will show the viability of using graphics processing units in real time systems control.
Identifying the Lack of Energy-Conscious Behaviour in Clinical and Non-Clinical Settings: An NHS Case Study
Authors: Taha Ahmad , Hopthrow Tim , Wu Ruiheng , Adams Neil , Brown Jessica , Zoha Ahmed , Abbasi Qammer H. , Imran Muhammad Ali , Krabicka Jan
Published: October 2021

The race against climate change has been a great challenge for years, and the UK government has taken serious steps towards achieving the net-zero carbon target by 2050. Technology is leading the way and innovation is believed to be a key solution. Nevertheless, tackling the issue, by attempting to limit the waste in energy, due to negative energy usage behaviour, has proven to be a successful approach that is capable of complementing other technology-based initiatives. The first step towards this is to promote energy-conscious behaviour and pinpoint where savings can be made. Thereby, this paper contributes to the existing literature, by presenting a new methodology to identify potential energy waste and negative energy usage behaviour in an NHS hospital. The paper presents an analysis of electricity consumption vs occupancy during minimal consumption periods (i.e, bank holidays and weekends) and it presents a log of equipment left switched on outside of working hours, in order to highlight the level of energy-conscious behaviour. The results revealed that the proposed technique is not only able to identify negative energy usage behaviour amongst the hospital staff but helps identify areas where immediate energy savings can be made, with potential savings of more than 30,000 pounds, if action is taken.
A Comprehensive Overview of Hydrogen-Fueled Internal Combustion Engines: Achievements and Future Challenges
Authors: Stępień Zbigniew
Published: October 2021

This paper provides a comprehensive review and critical analysis of the latest research results in addition to an overview of the future challenges and opportunities regarding the use of hydrogen to power internal combustion engines (ICEs). The experiences and opinions of various international research centers on the technical possibilities of using hydrogen as a fuel in ICE are summarized. The advantages and disadvantages of the use of hydrogen as a solution are described. Attention is drawn to the specific physical, chemical, and operational properties of hydrogen for ICEs. A critical review of hydrogen combustion concepts is provided, drawing on previous research results and experiences described in a number of research papers. Much space is devoted to discussing the challenges and opportunities associated with port and direct hydrogen injection technology. A comparison of different fuel injection and ignition strategies and the benefits of using the synergies of selected solutions are presented. Pointing to the previous experiences of various research centers, the hazards related to incorrect hydrogen combustion, such as early pre-ignition, late pre-ignition, knocking combustion, and backfire, are described. Attention is focused on the fundamental importance of air ratio optimization from the point of view of combustion quality, NOx emissions, engine efficiency, and performance. Exhaust gas scrubbing to meet future emission regulations for hydrogen powered internal combustion engines is another issue that is considered. The article also discusses the modifications required to adapt existing engines to run on hydrogen. Referring to still-unsolved problems, the reliability challenges faced by fuel injection systems, in particular, are presented. An analysis of more than 150 articles shows that hydrogen is a suitable alternative fuel for spark-ignition engines. It will significantly improve their performance and greatly reduce emissions to a fraction of their current level. However, its use also has some drawbacks, the most significant of which are its high NOx emissions and low power output, and problems in terms of the durability and reliability of hydrogen-fueled engines.
Numerical Simulation of Multiphase Multicomponent Flow in Porous Media: Efficiency Analysis of Newton-Based Method
Authors: Imankulov Timur , Lebedev Danil , Matkerim Bazargul , Daribayev Beimbet , Kassymbek Nurislam
Published: October 2021

Newton’s method has been widely used in simulation multiphase, multicomponent flow in porous media. In addition, to solve systems of linear equations in such problems, the generalized minimal residual method (GMRES) is often used. This paper analyzed the one-dimensional problem of multicomponent fluid flow in a porous medium and solved the system of the algebraic equation with the Newton-GMRES method. We calculated the linear equations with the GMRES, the GMRES with restarts after every m steps—GMRES (m) and preconditioned with Incomplete Lower-Upper factorization, where the factors L and U have the same sparsity pattern as the original matrix—the ILU(0)-GMRES algorithms, respectively, and compared the computation time and convergence. In the course of the research, the influence of the preconditioner and restarts of the GMRES (m) algorithm on the computation time was revealed; in particular, they were able to speed up the program.
On a Casson Fluid Motion: Nonuniform Width Symmetric Channel and Peristaltic Flows
Authors: Guadagni Simone , Palade Liviu , Fusi Lorenzo , Farina Angiolo
Published: October 2021

Widely used for modeling biological fluids flows—in particular, blood vessel flows—a Casson flow is studied in a symmetric channel for which the aspect ratio enables one to use the lubrication approximation. Two flow driving conditions are prescribed: inlet–outlet pressure difference and peristaltic oscillations of the vessel walls. In both cases, starting from mass and momentum balance and using lubrication approximation, we investigate the conditions to be imposed on the driving mechanisms so that the inner plug does not come in touch with the walls. The study of the peristaltic flow is of great importance in view of its applications in physiology (including microcirculation applications).
Breakthrough Investigation of Advective and Diffusive Transport in a Porous Matrix with a Crack
Authors: Holzbecher Ekkehard
Published: October 2021

Fluid flow and transport processes in fractured porous media are of particular interest for geologists and in the material sciences. Here a systematic investigation is presented, dealing with a generic geometric set-up of a porous matrix with a crack. In such a combined porous medium/free fluid system flow patterns have been examined frequently, while the resulting transport patterns have attracted less attention. Using numerical modeling with finite elements the problem is approached using a dimensionless formulation. With a reduced number of dimensionless parameter combinations (Darcy-, Peclet- and Reynolds-numbers) solution dependencies are examined in parametric sweeps. Breakthrough curves are fitted in comparison to those of 1D model approaches, yielding effective diffusivities and velocities. The computations reveal highest sensitivity concerning the angle between crack axis and flow direction, followed by the Peclet number and the crack axes ratio. As a dimensionless representation is used the results are scale independent. Thus, they deliver estimations concerning effective heat and solute transport parameters that can be relevant in all application fields.
Phytosterol Profiling of Apiaceae Family Seeds Spices Using GC-MS
Authors: Saini Ramesh Kumar , Song Min-Ho , Yu Ji-Woo , Shang Xiaomin , Keum Young-Soo
Published: October 2021

Phytosterols are nutritionally vital phytoconstituent owing to their cholesterol (low-density plasma lipoprotein-cholesterol, LDL-C)-lowering, anti-inflammatory, and antioxidant properties. Among the widely used spices and herbs, the seeds spices of the Apiaceae family represented the healthiest fatty acid profile. Thus, to explore the other health-beneficial lipids, the present study was aimed to analyze the phytosterol profile of eight seed spices of the Apiaceae family, utilizing gas chromatography (GC)-mass spectrometry (MS). The sterols contents calculated on an oil (mg/100 g of oil) and spice weight (mg/100 g spices; dry weight) basis varied significantly among the seed spices (p < 0.05; Turkey HSD). The β-sitosterol and stigmasterol were the most dominating sterols among the studied spices, together accounted for 40.3 (Ajwain) to 69.8% (celery) of total sterols in the seed oil. Among the studied spices, the oil extracted from caraway seeds showed the highest total sterols (602.2 mg/100 g of oil). Interestingly, based on spice weight, fennel seeds also showed the similar highest number of total sterols (134.2 mg/100 g in fennel and 133.3 mg/100 g in caraway), owing to the high contents of oil (25.9%) in fennel seeds. Overall, celery, caraway, fennel, and anise seeds oil are rich sources of health-beneficial phytosterols.
Efficacy of Biopolymer/Starch Based Antimicrobial Packaging for Chicken Breast Fillets
Authors: Yusof Noor L. , Mutalib Noor-Azira Abdul , Nazatul U. K. , Nadrah A. H. , Aziman Nurain , Fouad Hassan , Jawaid Mohammad , Ali Asgar , Kian Lau Kia , Sain Mohini
Published: October 2021

Food contamination leading to the spoilage and growth of undesirable bacteria, which can occur at any stage along the food chain, is a significant problem in the food industry. In the present work, biopolymer polybutylene succinate (PBS) and polybutylene succinate/tapioca starch (PBS/TPS) films incorporating Biomaster-silver (BM) and SANAFOR® (SAN) were prepared and tested as food packaging to improve the lifespan of fresh chicken breast fillets when kept in a chiller for seven days. The incorporation of BM and SAN into both films demonstrated antimicrobial activity and could prolong the storability of chicken breast fillets until day 7. However, PBS + SAN 2%, PBS/TPS + SAN 1%, and PBS/TPS + SAN 2% films showed the lowest microbial log growth. In quality assessment, incorporation of BM and SAN into both film types enhanced the quality of the chicken breast fillets. However, PBS + SAN 1% film showed the most notable enhancement of chicken breast fillet quality, as it minimized color variation, slowed pH increment, decreased weight loss, and decelerated the hardening process of the chicken breast fillets. Therefore, we suggest that the PBS + SAN and PBS/TPS + SAN films produced in this work have potential use as antimicrobial packaging in the future.
Lactobacillus rhamnosus Probio-M9 Improves the Quality of Life in Stressed Adults by Gut Microbiota
Authors: Zheng Yan , Yu Zhongjie , Zhang Wenyi , Sun Tiansong
Published: October 2021

Objective: To evaluate the effect of the probiotic, Lactobacillus rhamnosus Probio-M9 (Probio-M9), on the quality of life in stressed adults. Methods: Twelve postgraduate student volunteers were recruited. Six volunteers received oral Probio-M9 for 21 days, while the remaining six received a placebo instead. Fecal samples were collected from the volunteers before and after the intervention. Metagenomic sequencing, nontargeted metabonomics, and quality-of-life follow-up questionnaires were used to evaluate the impact of Probio-M9 consumption on the gut microbiota and life quality of the volunteers. Results: Probio-M9 improved the psychological and physiological quality-of-life symptoms significantly in stressed adults (p < 0.05). The probiotic intervention was beneficial in increasing and maintaining the diversity of gut microbiota. The abundance of Barnesiella and Akkermansia increased in the probiotics group. The feature metabolites of pyridoxamine, dopamine, and 5-hydroxytryptamine (5-HT) were positively correlated with Barnesiella and Akkermansia levels, which might be why the mental state of the volunteers in the probiotic group improved after taking Probio-M9. Conclusions: We identified that oral Probio-M9 can regulate the stability of gut microbiota and affect the related beneficial metabolites, thereby affecting the quality of life (QoL) of stressed adults. Probio-M9 might improve the psychological and physiological quality of life in stressed adults via the gut-brain axis pathway. The causal relationship should be further explored in future studies.
Characterization and Discrimination of Key Aroma Compounds in Pre- and Postrigor Roasted Mutton by GC-O-MS, GC E-Nose and Aroma Recombination Experiments
Authors: Liu Huan , Hui Teng , Fang Fei , Ma Qianli , Li Shaobo , Zhang Dequan , Wang Zhenyu
Published: October 2021

The key aroma compounds in the pre- and postrigor roasted mutton were studied in this study. The results showed that 33 and 30 odorants were detected in the pre- and postrigor roasted mutton, respectively. Eight aroma compounds, including 3-methylbutanal, pentanal, hexanal, heptanal, octanal, nonanal, 1-octen-3-ol, and 2-pentylfuran, were confirmed as key odorants by aroma recombination and omission experiments. The aroma profiles of pre- and postrigor roasted mutton both presented great fatty, roasty, meaty, grassy, and sweet odors. Particularly, the concentrations of hexanal, heptanal, octanal, nonanal, 1-octen-3-ol, and 2-pentylfuran in postrigor roasted mutton were significantly higher (p < 0.05) than those in the prerigor roasted mutton. The postrigor back strap was more suitable for roasting than the prerigor back strap. The pre- and postrigor roasted mutton could be obviously discriminated based on the aroma compounds by orthogonal partial least squares discrimination analysis (OPLS-DA) and principal component analysis (PCA). Hexanal and 1-octen-3-ol might potential markers for the discrimination of the pre- and postrigor roasted mutton.
Traditional Fermented Beverages of Mexico: A Biocultural Unseen Foodscape
Authors: Ojeda-Linares César , Álvarez-Ríos Gonzalo D. , Figueredo-Urbina Carmen Julia , Islas Luis Alfredo , Lappe-Oliveras Patricia , Nabhan Gary Paul , Torres-García Ignacio , Vallejo Mariana , Casas Alejandro
Published: October 2021

Mexico is one of the main regions of the world where the domestication of numerous edible plant species originated. Its cuisine is considered an Intangible Cultural Heritage of Humanity and ferments are important components but have been poorly studied. Traditional fermented foods are still diverse, but some are endangered, requiring actions to promote their preservation. Our study aimed to (1) systematize information on the diversity and cultural history of traditional Mexican fermented beverages (TMFB), (2) document their spatial distribution, and (3) identify the main research trends and topics needed for their conservation and recovery. We reviewed information and constructed a database with biocultural information about TMFB prepared and consumed in Mexico, and we analyzed the information through network approaches and mapped it. We identified 16 TMFB and 143 plant species involved in their production, species of Cactaceae, Asparagaceae, and Poaceae being the most common substrates. Microbiological research has been directed to the potential biotechnological applications of Lactobacillus, Bacillus, and Saccharomyces. We identified a major gap of research on uncommon beverages and poor attention on the cultural and technological aspects. TMFB are dynamic and heterogenous foodscapes that are valuable biocultural reservoirs. Policies should include their promotion for conservation. The main needs of research and policies are discussed.
Seasonal Nutritional Profile of Gelidium corneum (Rhodophyta, Gelidiaceae) from the Center of Portugal
Authors: Cavaco Mário , Duarte Adriana , Freitas Marta V. , Afonso Clélia , Bernardino Susana , Pereira Leonel , Martins Mendelson , Mouga Teresa
Published: October 2021

Gelidium corneum is a well-known agarophyte, harvested worldwide for its high agar quality. However, the species also exhibits an interesting nutritional profile, but with seasonal variations. Therefore, to evaluate the nutritional value of G. corneum, ash, crude protein, total lipids, and carbohydrates were analyzed at different times of the year. The heavy metals mercury, arsenic, lead, cadmium, and tin, as well as iodine were also measured. Finally, the seasonal antioxidant capacity of G. corneum extracts was evaluated. Our results indicate that the biomass is rich in protein (up to 16.25 ± 0.33%) and carbohydrates (up to 39.5 ± 3.29%), and low in lipids (up to 2.75 ± 0.28%), and especially in the summer, the AI, TI indexes, n-6/n-3 and h/H ratios (0.93, 0.6, 0.88 and 1.08, respectively) are very interesting. None of the contaminants exceeded the legally established limits, and the iodine values were adequate for a healthy diet. Finally, the antioxidant capacity is fair, with the DPPH ≤ 10.89 ± 1.46%, and ABTS ≤ 13.90 ± 1.54% inhibition, FRAP ≤ 0.91 ± 0.22 AAE.g−1, and TPC ≤ 6.82 ± 0.26 GAE.g−1. The results show that G. corneum is an attractive resource, with potential use as food or as a food supplement.
Impact of Purchase Preference, Perceived Value, and Marketing Mix on Purchase Intention and Willingness to Pay for Pork
Authors: Kung Mei-Ling , Wang Jiun-Hao , Liang Chaoyun
Published: October 2021

This study explored the effects of purchase preference, perceived value, and marketing mix on consumers’ purchase intention and willingness to pay for pork in Taiwan. A questionnaire was distributed to pork consumers in an online platform, and a total of 1042 valid samples were collected. An analysis of the questionnaire responses revealed three purchase preference factors, namely flavour, certification marks, and added features; four perceived value factors, namely functional, social, conditional, and emotional value; and four marketing mix factors, namely promotion, convenience, product, and price marketing. Functional value, purchase frequency, conditional value, and product marketing positively affected purchase intention. Promotional marketing, monthly disposable income, and social value were the main positive factors in increased willingness to pay. Consumers who were men, had a lower educational level, purchased large quantities of pork at one time, or frequently purchased pork exhibited higher purchase intention than did other consumers. Those who were men, had higher educational attainment, had a higher monthly disposable income, or held a management position were more willing to purchase pork at a premium price.
Development and Characterization of Extrudates Based on Rapeseed and Pea Protein Blends Using High-Moisture Extrusion Cooking
Authors: Zahari Izalin , Ferawati Ferawati , Purhagen Jeanette K. , Rayner Marilyn , Ahlström Cecilia , Helstad Amanda , Östbring Karolina
Published: October 2021

Rapeseed protein is not currently utilized for food applications, although it has excellent physicochemical, functional, and nutritional properties similar to soy protein. Thus, the goal of this study was to create new plant-based extrudates for application as high-moisture meat analogs from a 50:50 blend of rapeseed protein concentrate (RPC) and yellow pea isolate (YPI) using high-moisture-extrusion (HME) cooking with a twin-screw extruder to gain a better understanding of the properties of the protein powders and resulting extrudates. The effects of extrusion processing parameters such as moisture content (60%, 63%, 65%, 70%), screw speed (500, 700, and 900 rpm), and a barrel temperature profile of 40–80–130–150 °C on the extrudates’ characteristics were studied. When compared to the effect of varying screw speeds, targeted moisture content had a larger impact on textural characteristics. The extrudates had a greater hardness at the same moisture content when the screw speed was reduced. The specific mechanical energy (SME) increased as the screw speed increased, while increased moisture content resulted in a small reduction in SME. The lightness (L*) of most samples was found to increase as the target moisture content increased from 60% to 70%. The RPC:YPI blend was equivalent to proteins produced from other sources and comparable to the FAO/WHO standard requirements.
Development of a New Monoclonal Antibody against Brevetoxins in Oyster Samples Based on the Indirect Competitive Enzyme-Linked Immunosorbent Assay
Authors: Zhang Xiya , Ding Mingyue , Zhang Chensi , Mao Yexuan , Wang Youyi , Li Peipei , Jiang Haiyang , Wang Zhanhui , Yu Xuezhi
Published: October 2021

The consumption of shellfish contaminated with brevetoxins, a family of ladder-frame polyether toxins formed during blooms of the marine dinoflagellate Karenia brevis, can cause neurotoxic poisoning, leading to gastroenteritis and neurotoxic effects. To rapidly monitor brevetoxin levels in oysters, we generated a broad-spectrum antibody against brevetoxin 2 (PbTx-2), 1 (PbTx-1), and 3 (PbTx-3) and developed a rapid indirect competitive enzyme-linked immunosorbent assay (icELISA). PbTx-2 was reacted with carboxymethoxylamine hemihydrochloride (CMO) to generate a PbTx-2-CMO hapten and reacted with succinic anhydride (HS) to generate the PbTx-2-HS hapten. These haptens were conjugated to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA) to prepare immunogen and coating antigen reagents, respectively, using the active ester method. After immunization and cell fusion, a broad-spectrum monoclonal antibody (mAb) termed mAb 1D3 was prepared. The 50% inhibitory concentration (IC50) values of the icELISA for PbTx-2, PbTx-1, and PbTx-3 were 60.71, 52.61, and 51.83 μg/kg, respectively. Based on the broad-spectrum mAb 1D3, an icELISA was developed to determine brevetoxin levels. Using this approach, the limit of detection (LOD) for brevetoxin was 124.22 μg/kg and recoveries ranged between 89.08% and 115.00%, with a coefficient of variation below 4.25% in oyster samples. These results suggest that our icELISA is a useful tool for the rapid monitoring of brevetoxins in oyster samples.