bluuebunny/crossref_metadata_2025 huggingface dataset:
Effectiveness and safety of a one-yearly elongation approach of growing rods in the treatment of early-onset scoliosis: A case series of 40 patients with definitive fusion
Authors: Vittoria Francesca , Ceconi Viola , Fantina Lisa , Barbi Egidio , Carbone Marco
Published: November 2022

Background

Early onset scoliosis (EOS) can lead to marked spine and chest wall deformity and often to profound cardiopulmonary compromise. Nowadays, treatment benefits from the possibility of a growth-friendly surgical approach to avoid early spinal fusion. Growing rod spinal implants allow maximizing spine and thorax growth during childhood, performing lengthening procedures traditionally approximately every 6 months.

Methods

We retrospectively evaluated 40 patients affected by EOS who underwent growing rod implantations from 2000 to 2020. A 1-year interval between lengthening procedures was adopted. Data about the age at the first and final surgeries, T1-T12 length pre- and post-surgery, T1-S1 pre- and post-surgery, major coronal curve, pre- and post-surgery rate of complications, and unplanned surgeries were collected and compared with those reported in the literature to determinate the effectiveness and safety of this long period between distractions.

Results

The lengthening procedures were performed, on average, every 12.3 months; children underwent an average of 4.6 lengthening procedures each. Major curve pre-first surgery was 78°, post-first surgery 45°, pre-final surgery 55°, and post-final surgery 43°. The mean absolute difference between pre-initial to post-final major curve was 35°, representing a mean relative difference of 42%. On average, the T1-T12 segment measured 15 cm before the first surgery and 24 cm after the final surgery, while the T1-S1 segment was 25 cm before the growing rods implantation and reached 37 cm after treatment. During treatment, the adverse events affected 27 of the 40 total patients (67%) who experienced at least one complication. No differences were shown concerning both outcomes and complications, comparing these data with the available literature concerning most frequent elongations. This approach avoided four to five surgical procedures in this population.

Conclusion

Our results related to deformity correction and complication rate are comparable with those found in the literature, where lengthening procedures are performed approximately every 6 months rather than with a 1-year interval between distractions. We also demonstrate a higher risk of complications for patients with implants before the age of 6.
Novel SRY-box transcription factor 9 variant in campomelic dysplasia and the location of missense and nonsense variants along the protein domains: A case report
Authors: Calvache Carlos A. , Vásquez Estefanía C. , Romero Vanessa I. , Hosomichi Kazuyoshi , Pozo Juan C.
Published: November 2022

Background

Campomelic dysplasia (CD) is a rare disorder that involves the skeletal and genital systems. This condition has been associated with a diverse set of mutations in the SRY-box transcription factor 9 (SOX9) gene.

Case presentation

We herein report a case involving a 4-year-old female patient with CD, female sex reversal, type 1 Arnold–Chiari malformation, and bilateral conductive hearing loss and investigate the causal mutation. Whole-exome sequencing analysis detected a novel Trp115X* variant in the SOX9 gene. We performed a literature review of the reported cases and demonstrated that the missense variants were located only in the self-dimerization domain (DIM) and high-mobility group box domains. We also reported that variants in the DIM domain do not cause sex reversal and identified that the amino acid sequences that were mutated in the patients with campomelic dysplasia are evolutionarily conserved among primates.

Conclusions

We suggest that missense variants cannot be located in the K2, PQA, and PQS given that these domains function critically for transcriptional activation or repression of target genes and evolve under purifying selection.
The pharmacokinetics profiles, pharmacological properties, and toxicological risks of dehydroevodiamine: A review
Authors: Fu Shubin , Liao Liying , Yang Yi , Bai Yan , Zeng Yan , Wang Haoyu , Wen Jianxia
Published: November 2022

Dehydroevodiamine (DHE) is a quinazoline alkaloid isolated from Evodiae Fructus (EF, Wuzhuyu in Chinese, Rutaceae family), a well-known traditional Chinese medicine (TCM) which is clinically applied to treat headache, abdominal pain, menstrual pain, abdominal distension, vomiting, acid regurgitation, etc. Modern research demonstrates that DHE is one of the main components of EF. In recent years, DHE has received extensive attention due to its various pharmacological activities. This review is the first to comprehensively summarize the current studies on pharmacokinetics profiles, pharmacological properties, and toxicological risks of DHE in diverse diseases. Pharmacokinetic studies have shown that DHE has a relatively good oral absorption effect in the mean concentration curves in rat plasma and high absorption in the gastrointestinal tract. In addition, distribution re-absorption and enterohepatic circulation may lead to multiple blood concentration peaks of DHE in rat plasma. DHE possesses a wide spectrum of pharmacological properties in the central nervous system, cardiovascular system, and digestive system. Moreover, DHE has anti-inflammatory effects via downregulating pro-inflammatory cytokines and inflammatory mediators. Given the favorable pharmacological activity, DHE is expected to be a potential drug candidate for the treatment of Alzheimer’s disease, chronic stress, amnesia, chronic atrophic gastritis, gastric ulcers, and rheumatoid arthritis. In addition, toxicity studies have suggested that DHE has proarrhythmic effects and can impair bile acid homeostasis without causing hepatotoxicity. However, further rigorous and well-designed studies are needed to elucidate the pharmacokinetics, pharmacological effects, potential biological mechanisms, and toxicity of DHE.
Curcumol repressed cell proliferation and angiogenesis via SP1/mir-125b-5p/VEGFA axis in non-small cell lung cancer
Authors: Ma Changju , Tang Xiaojuan , Tang Qing , Wang Shiyan , Zhang Junhong , Lu Yue , Wu Jingjing , Han Ling
Published: November 2022

NSCLC (non-small cell lung cancer) is one of the most common and lethal malignant tumors, with low 5-year overall survival rate. Curcumol showed antitumor activity in several cancers, but evidence about its effect on NSCLC remains unclear. In the present study, we found that Curcumol markedly inhibited NSCLC cells proliferation, migration and invasion. Endothelial cells are an important part of tumor microenvironment. Tube formation assay and wound healing assay indicated that A549 derived conditioned medium affected HUVECs (human umbilical vein endothelial cells). Mechanistically, Curcumol downregulated the expression of SP1 (specificity protein 1) while upregulated miR-125b-5p, followed by decreasing VEGFA expression in NSCLC cells. Furthermore, overexpression of SP1 partially reversed the inhibitory effect of Curcumol on A549 and H1975 cell viability and VEGFA expression. Inhibition of miR-125b-5p presented similar effect. Interestingly, there was mutual modulation between SP1 and miR-125b-5p. Collectively, our study revealed that Curcumol inhibited cell growth and angiogenesis of NSCLC in vitro and in vivo, possibly through SP1/miR-125b-5p/VEGFA regulatory mechanism. These findings may provide effective therapy strategies for NSCLC treatment.
Zhilong Huoxue Tongyu Capsules' Effects on ischemic stroke: An assessment using fecal 16S rRNA gene sequencing and untargeted serum metabolomics
Authors: Wang Raoqiong , Liu Mengnan , Ren Guilin , Luo Gang , Wang Zhichuan , Ge Zhengxin , Pu Qingrong , Ren Wei , Yang Sijin
Published: November 2022

Zhilong Huoxue Tongyu capsule (ZHTC) is an effective traditional Chinese medicine compound for the treatment of ischemic stroke, which is widely used in clinical ischemic stroke patients. However, it is uncertain whether ZHTC affects ischemic stroke through gut microbiota and serum metabolites. In this study, a rat model of middle cerebral artery occlusion (MCAO) was prepared. By evaluating motor nerve function score, cerebral infarct size, brain tissue damage and intestinal barrier damage, it was found that ZHTC improved stroke-related symptoms in MCAO rats. Using 16S rRNA gene sequencing, fecal microbial transplantation (FMT), untargeted metabolomics, and spearman correlation analysis of gut microbiota and serum metabolites, we found that ZHTC can regulate the abundance of p_Firmicutes, p_Bacteroidota,p_Proteobacteria, g_Prevotella, and g_Lactobacillus, and regulated 23 differential metabolites. Spearman correlation analysis found that Arginine was positively correlated with p_Firmicutes, o_Clostridiales, c_Clostridia, and negatively correlated with p_Bacteroidetes, c_Bacteroidia,o_Bacteroidales; L-Lysine was negatively correlated with f_Christensenellaceae; L-methionine was positively correlated with o_Lactobacillales, f_Lactobacillaceae, and g_Lactobacillus. Altogether, this study shows for the first time that ZHTC can ameliorate ischemic stroke by modulating gut microbiota and metabolic disturbances. This lays the foundation for further revealing the causal relationship between ZHTC, gut dysbiosis, plasma metabolite levels and ischemic stroke, and provides a scientific explanation for the ameliorating effect of ZHTC on ischemic stroke.
Identification of TGF-β signaling-related molecular patterns, construction of a prognostic model, and prediction of immunotherapy response in gastric cancer
Authors: Zeng Cheng , He Rong , Dai Yuyang , Lu Xiaohuan , Deng Linghui , Zhu Qi , Liu Yu , Liu Qian , Lu Wenbin , Wang Yue , Jin Jianhua
Published: November 2022

Background: TGF-β signaling pathway plays an essential role in tumor progression and immune responses. However, the link between TGF-β signaling pathway-related genes (TSRGs) and clinical prognosis, tumor microenvironment (TME), and immunotherapy in gastric cancer is unclear.

Methods: Transcriptome data and related clinical data of gastric cancer were downloaded from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, and 54 TSRGs were obtained from the Molecular Signatures Database (MSigDB). We systematically analyzed the expression profile characteristics of 54 TSRGs in 804 gastric cancer samples and examined the differences in prognosis, clinicopathological features, and TME among different molecular subtypes. Subsequently, TGF-β-related prognostic models were constructed using univariate and least absolute shrinkage and selection operator (LASSO) Cox regression analysis to quantify the degree of risk in each patient. Patients were divided into two high- and low-risk groups based on the median risk score. Finally, sensitivity to immune checkpoint inhibitors (ICIs) and anti-tumor agents was assessed in patients in high- and low-risk groups.

Results: We identified two distinct TGF-β subgroups. Compared to TGF-β cluster B, TGF-β cluster A exhibits an immunosuppressive microenvironment with a shorter overall survival (OS). Then, a novel TGF-β-associated prognostic model, including SRPX2, SGCE, DES, MMP7, and KRT17, was constructed, and the risk score was demonstrated as an independent prognostic factor for gastric cancer patients. Further studies showed that gastric cancer patients in the low-risk group, characterized by higher tumor mutation burden (TMB), the proportion of high microsatellite instability (MSI-H), immunophenoscore (IPS), and lower tumor immune dysfunction and exclusion (TIDE) score, had a better prognosis, and linked to higher response rate to immunotherapy. In addition, the risk score and anti-tumor drug sensitivity were strongly correlated.

Conclusion: These findings highlight the importance of TSRGs, deepen the understanding of tumor immune microenvironment, and guide individualized immunotherapy for gastric cancer patients.
Effects of transcranial alternating current stimulation on motor performance and motor learning for healthy individuals: A systematic review and meta-analysis
Authors: Hu Kun , Wan Ruihan , Liu Ying , Niu Maolin , Guo Jianrui , Guo Feng
Published: November 2022

Objective:Previous behavioral studies have reported the potential of transcranial alternating current stimulation in analyzing the causal relationship between neural activity and behavior. However, the efficacy of tACS on motor performance and learning in healthy individuals remains unclear. This systematic reviewexamines the effectiveness of tACS on motor performance and motor learning in healthy individuals.

Methods: Literature was systematically searched through the Cochrane Library, PubMed, EMBASE, and Web of Science until 16 October 2022. Studies were eligible for review if they were randomized, parallel, or crossover experimental designs and reported the efficacy of tACS on motor performance and motor learning in healthy adults. Review Manager 5.3 was used to evaluate the methodological quality and analyze the combined effect.

Results: Ten studies (270 participants) met all the inclusion criteria. The results showed that motor performance was not significantly greater than that with sham tACS stimulation [I2 = 44%, 95% CI (–0.01, 0.35), p = 0.06, standardized mean difference = 0.17], whereas motor learning ability improved significantly [I2 = 33%, 95% CI (−1.03, −0.31), p = 0.0002, SMD = −0.67]. Subgroup analysis found that gamma bend tACS could affect the changes in motor performance (I2 = 6%, 95% CI (0.05, 0.51), p = 0.02, SMD = 0.28), and online tACS did as well [I2 = 54%, 95% CI (0.12, 0.56), p = 0.002, SMD = 0.34].

Conclusion: The results showed that tACS effectively improves motor performance (gamma band and online mode) and motor learning in healthy individuals, which indicates that tACS may be a potential therapeutic tool to improve motor behavioral outcomes. However, further evidence is needed to support these promising results.

Systematic Review Registration: PROSPERO, identifier CRD42022342884
SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogen Phytophthora infestans
Authors: Mu Yang , Guo Xiao , Yu Jian , Wang Ruxun , Liu Zeng , Hu Kefan , Song Jingyi , Chen Lin , Song Botao , Du Juan
Published: November 2022

Potato is the most important non-grain food in the world, while late blight caused by Phytophthora infestans seriously threatens the production of potato. Since pathogen-associated molecular patterns (PAMPs) are relatively conserved, PAMP-triggered immunity (PTI) can provide durable resistance to late blight for potato. However, knowledge of the regulatory mechanisms of PTI against oomycete pathogens at protein levels remains limited due to the small number of identified proteins. In the present work, changes in the proteome profile of Nicotiana benthamiana leaves upon P. infestans PAMP induction were examined using the SWATH-MS (sequential windowed acquisition of all theoretical mass spectra) approach, which provides quantification of protein abundances and large-scale identification of PTI-related proteins. A total of 4401 proteins have been identified, of which 1429 proteins were differentially expressed at least at one time point of 8, 12, 24 and 48 h after PAMP induction, compared with the expression at 0 h when immediately after PAMP induction. They were further analyzed by expression clustering and gene ontology (GO) enrichment analysis. Through functional verification, six novel DEPs of 19 candidates were proved to be involved in PTI responses, including mitochondrial phosphate carrier protein (MPT) 3, vesicle-associated membrane protein (VAMP) 714, lysophospholipase (LysoPL) 2, ascorbate peroxidase (APX) 1, heat shock 70 kDa protein (HSP) 2 and peptidyl-prolyl cis-trans isomerase FKBP (FKBP) 15-1. Taken together, the time course approach and the resulting large-scale proteomic analyses have enlarged our understanding of PTI mechanisms and provided a valuable resource for the discovery of complex protein networks involved in the resistance response of potato to late blight.
A mass spectrometry imaging approach on spatiotemporal distribution of multiple alkaloids in Gelsemium elegans
Authors: Wu Zi-Han , Wang Ruo-Zhong , Sun Zhi-Liang , Su Yi , Xiao Lang-Tao
Published: November 2022

Gelsemium elegans contains multiple alkaloids with pharmacological effects, thus researchers focus on the identification and application of alkaloids extracted from G. elegans. Regretfully, the spatiotemporal distribution of alkaloids in G. elegans is still unclear. In this study, the desorption electrospray ionization mass spectrometry imaging (DESI-MSI) was applied to simultaneously analyze the distribution of pharmacologically important alkaloids in different organ/tissue sections of G. elegans at different growth stages. Finally, 23 alkaloids were visualized in roots, stems and leaves at seedling stage and 19 alkaloids were observed at mature stage. In mature G. elegans, 16 alkaloids were distributed in vascular bundle region of mature roots, 15 alkaloids were mainly located in the pith region of mature stems and 2 alkaloids were enriched in epidermis region of mature stems. A total of 16 alkaloids were detected in leaf veins of mature leaves and 17 alkaloids were detected in shoots. Interestingly, diffusion and transfer of multiple alkaloids in tissues have been observed along with the development and maturation. This study comprehensively characterized the spatial metabolomics of G. elegans alkaloids, and the spatiotemporal distribution of alkaloid synthesis. In addition, the results also have reference value for the development and application of Gelsemium elegans and other medicinal plants.
Natural products as sources of acetylcholinesterase inhibitors: Synthesis, biological activities, and molecular docking studies of osthole-based ester derivatives
Authors: Yu Xiang , Zhang Yan , Zhang Minjie , Chen Yafang , Yang Wude
Published: November 2022

Osthole is a natural coumarin compound which isolated from Cnidium monnieri (L.) Cusson, has extensive pharmacological activities and could be used as a leading compound for drug research and development. In a continuous effort to develop new acetylcholinesterase inhibitors from natural products, eighteen osthole esters were designed, synthesized, and confirmed by 1H NMR, 13C NMR and HRMS. The anti-AChE activity of These derivatives was measured at a concentration of 1.0 mol/mL in vitro by Ellman's method, and the result showed that 4m and 4o had moderate inhibitory activities with 68.8% and 62.6%, respectively. Molecular docking study results further revealed AChE interacted optimally with docking poses 4m and 4o. Network pharmacology also predicted that compound 4m could be involved in Ras signaling pathway, which made it a potential therapeutic target of AD.
Literature review on generational inheritance model and applications for Chinese family businesses
Authors: Li Shuqi
Published: November 2022

At present, Chinese family businesses have ushered in the peak period of generational inheritance, so the issue of family business inheritance has become more and more important. The generational inheritance of family businesses is not something that can be accomplished in a short period of time, but requires a long process. There are many influencing factors, and how to choose an effective inheritance model for family businesses has always been the focus of academic circles and practitioners. At present, there is no unified conclusion about the choice of family business generational inheritance model and the factors that affect the model choice. By sorting out a large number of domestic and foreign literature, this paper summarizes the predecessors’ perspectives on the inheritance model, discusses various driving factors that may affect the current inheritance model. By further analyzes the current influencing factors on the inheritance model, this paper discusses that inheritance intention of successor may be a research perspective that has not been valued, thus proposing an expandable direction in the field of family business inheritance research.
Gross anatomical features of the insular cortex in schizophrenia and schizotypal personality disorder: Potential relationships with vulnerability, illness stages, and clinical subtypes
Authors: Takahashi Tsutomu , Sasabayashi Daiki , Takayanagi Yoichiro , Furuichi Atsushi , Kobayashi Haruko , Yuasa Yusuke , Noguchi Kyo , Suzuki Michio
Published: November 2022

Introduction

Patients with schizophrenia have a higher number of insular gyri; however, it currently remains unclear whether the brain characteristics of patients with schizotypal personality disorder (SPD), a mild form of schizophrenia, are similar. It is also unknown whether insular gross anatomical features are associated with the illness stages and clinical subtypes of schizophrenia.

Materials and methods

This magnetic resonance imaging study examined gross anatomical variations in the insular cortex of 133 patients with schizophrenia, 47 with SPD, and 88 healthy controls. The relationships between the insular gross anatomy and schizophrenia subgroups (71 first-episode and 58 chronic groups, 38 deficit and 37 non-deficit subtype groups) were also investigated.

Results

The number of insular gyri was higher in the schizophrenia and SPD patients than in the controls, where the patients were characterized by well-developed accessory, middle short, and posterior long insular gyri. The insular gross anatomy did not significantly differ between the first-episode and chronic schizophrenia subgroups; however, the relationship between the developed accessory gyrus and more severe positive symptoms was specific to the first-episode group. The prevalence of a right middle short gyrus was higher in the deficit schizophrenia group than in the non-deficit group.

Discussion

These findings suggest that schizophrenia and SPD patients may share an altered insular gross morphology as a vulnerability factor associated with early neurodevelopmental anomalies, which may also contribute to positive symptomatology in the early illness stages and clinical subtypes of schizophrenia.
Case report: Recovery of hallucinations and cognitive impairment after administration of donepezil in a patient with schizophrenia and carbon monoxide poisoning
Authors: Baek Seon-Hwa , Kim Ju-Wan , Lee Yun Young , Kim Ju-Yong , Kim Sung-Wan , Kim Jae-Min
Published: November 2022

Objectives

This report aims to introduce a rare case of a dramatic recovery by donepezil with a patient with schizophrenia who suffered from remaining psychotic symptoms despite proper treatment and had a cognitive impairment by carbon monoxide (CO) poisoning sequelae.

Case report

A 38-year-old male who developed schizophrenia 2 years ago had attempted suicide via CO inhalation due to his uncontrolled symptoms. He was hospitalized with delayed neurological sequelae (DNS). Though hyperbaric oxygen therapy (HBOT) was applied 10 times, his cognitive impairment did not recover. Surprisingly, with 5–10 mg donepezil, both cognitive function and the psychotic symptoms of the patient remarkably improved.

Conclusion

This case showed a good response of donepezil for a patient with schizophrenia and CO-induced DNS after ineffective HBOT. Although the mechanism of the phenomenon is unclear, it can be possible reasons that the neuroprotective effect of donepezil and white matter insult by CO poisoning.
Impacts of psychological wellbeing with HIV/AIDS and cancer among sexual and gender minorities: A systematic review and meta-analysis
Authors: Chan Alex Siu Wing , Leung Lok Man , Li Jane Siu Fan , Ho Jacqueline Mei Chi , Tam Hon Lon , Hsu Wing Leung , Iu April Nicole On Sang , Tang Patrick Ming Kuen , Yan Elsie
Published: November 2022

Background

The agony and economic strain of cancer and HIV/AIDS therapies severely impact patients' psychological wellbeing. Meanwhile, sexual minorities experience discrimination and mental illness. LGBT individuals with cancer and HIV/AIDS play two roles. It is important to understand and examine this groups mental wellbeing.

Objective

The purpose of this study is to synthesize current studies on the impact of HIV/AIDS and cancer on LGBT patients' psychological wellbeing.

Methods

This research uses a systematic literature review at first and later stage a meta-analysis was run on the same review. In this study, data from Google academic and Web of Science has been used to filter literature. PRISMA 2020 Flow Diagram seeks research on LGBT cancer and HIV/AIDS patients. The above sites yielded 370 related papers, some of which were removed due to age or inaccuracy. Finally, meta-analyses was done on 27 HIV/AIDS and 33 cancer patients's analyse.

Results

The research included 9,898 LGBT cancer sufferers with AIDS and 14,465 cancer sufferers with HIV/AIDS. Using meta-analysis, we discovered the gap in psychological wellbeing scores between HIV/AIDS LGBT and non-LGBT groups ranged from −10.86 to 15.63. The overall score disparity between the HIV/AIDS LGBT and non-LGBT groups was 1.270 (95% CI = 0.990–1.560, Z = 86.58, P < 0.1). The disparity in psychological wellbeing scores between cancer LGBT group and general group varies from −8.77 to 20.94 in the 34 papers examined in this study. Overall, the psychological wellbeing score disparity between the cancer LGBT subset and the general group was 12.48 (95% CI was 10.05–14.92, Test Z-value was 268.40, P-value was <0.1).

Conclusion

Inflammation and fibrosis in HIV/AIDS and cancer sufferers adversely affect their psychological wellbeing.
Oxygen A-band absorption spectroscopy with solar photon counting and lithium niobate nanophotonic circuits
Authors: Zhang Jiuyi , Sua Yong Meng , Hu Yongxiang , Ramanathan Jeevanandha , Huang Yu-Ping
Published: November 2022

Oxygen A-band measurements can provide important information about cloud top height, cloud physical and optical thickness, and surface atmospheric pressure. So far, O2 A-band measurements are typically made with spectrometers at a spectral resolution of 40 p.m. (such as in the OCO-2 satellite) or a coarser resolution. This paper reports a new CubeSat measurement concept with higher spectral resolution over the O2 A-band using integrated photonic circuits and solar photon counting techniques. An integrated Micro-ring filter (MRR) chip with 10 p.m. resonant linewidth with an extinction ratio of 25 dB or higher is designed, fabricated, and used for precise measurements of the atmospheric oxygen A-band absorption line-shapes around 770 nm. With solar-photon counting and using a narrow-band filter made of an integrated, fast-swept MRR on lithium niobate on insulator (LNOI), we have demonstrated a high-resolution measurement of the O2 A-band absorption spectrum, exhibiting good agreement with the HITRAN database.
Deep learning-based galaxy image deconvolution
Authors: Akhaury Utsav , Starck Jean-Luc , Jablonka Pascale , Courbin Frédéric , Michalewicz Kevin
Published: November 2022

With the onset of large-scale astronomical surveys capturing millions of images, there is an increasing need to develop fast and accurate deconvolution algorithms that generalize well to different images. A powerful and accessible deconvolution method would allow for the reconstruction of a cleaner estimation of the sky. The deconvolved images would be helpful to perform photometric measurements to help make progress in the fields of galaxy formation and evolution. We propose a new deconvolution method based on the Learnlet transform. Eventually, we investigate and compare the performance of different Unet architectures and Learnlet for image deconvolution in the astrophysical domain by following a two-step approach: a Tikhonov deconvolution with a closed-form solution, followed by post-processing with a neural network. To generate our training dataset, we extract HST cutouts from the CANDELS survey in the F606W filter (V-band) and corrupt these images to simulate their blurred-noisy versions. Our numerical results based on these simulations show a detailed comparison between the considered methods for different noise levels.
Animal model contributes to the development of intracranial aneurysm: A bibliometric analysis
Authors: Chen Jia , Liu Jing , Liu Xin , Zeng Chudai , Chen Zhou , Li Shifu , Zhang Qian
Published: November 2022

Introduction

Studies on intracranial aneurysms (IAs) using animal models have evolved for decades. This study aimed to analyze major contributors and trends in IA-related animal research using bibliometric analysis.

Methods

IA-related animal studies were retrieved from the Web of Science database. Microsoft Excel 2010, GraphPad Prism 6, VOSviewer, and CiteSpace were used to collect and analyze the characteristics of this field.

Results

A total of 273 publications were retrieved. All publications were published between 1976 and 2021, and the peak publication year is 2019. Rat model were used in most of the publications, followed by mice and rabbits. Japan (35.5%), the United States (30.0%), and China (20.1%) were the top three most prolific countries. Although China ranks third in the number of publications, it still lacks high-quality articles and influential institutions. Stroke was the most prolific journal that accepted publications related to IA research using animal models. Circulation has the highest impact factor with IA-related animal studies. Hashimoto N contributed the largest number of articles. Meng hui journal published the first and second highest cited publications. The keywords “subarachnoid hemorrhage,” “macrophage,” “rupture,” “mice,” “elastase,” “gene,” “protein,” “proliferation,” and “risk factors” might be a new trend for studying IA-related animal research.

Conclusions

Japan and the Unites States contributed the most to IA–related animal studies, in terms of both researchers and institutions. Although China ranks third in terms of the number of publications, it should strengthen the quality of its publications. Researchers should pay attention to the latest progress of Stroke, Journal of Neurosurgery, Neurosurgery, and Circulation for their high-quality IA-related animal studies. Using animal IA models, especially mice, to investigate the molecular mechanisms of IA may be the frontier topic now and in future.
Isolation, molecular typing and antimicrobial resistance of Clostridium difficile in dogs and cats in Lanzhou city of Northwest China
Authors: Wen Gui-Lin , Li Shi-Hong , Qin Zhe , Yang Ya-Jun , Bai Li-Xia , Ge Wen-Bo , Liu Xi-Wang , Li Jian-Yong
Published: November 2022

Clostridium difficile infection (CDI) in human and animals belonged usually to antibiotic-associated diarrhea, ranging in severity from mild to life-threatening intestinal tract illnesses. This study aimed to isolation and characterization, toxin genes test, molecular typing, and drug sensitivity of Clostridium difficile (C. difficile) which were isolated from clinical diseased dogs and cats. A total of 247 clinical samples were collected from five animal hospitals in Lanzhou City of Northwest China, of which dogs and cats accounted for 74.9% (185/247) and 25.1% (62/247), respectively. We successfully identified 24 C. difficile strains by 16S rRNA and Matrix-Assisted Laser Desorption/Ionization Time of Fight Mass Spectroscopy (MALDI-TOF-MS). 10.3% (19/185) of dogs and 8.1% (5/62) of cats were positive for C. difficile. Among them, 16 strains were toxic and 8 were non-toxic, with a toxic rate of 57.9% (11/19) in dogs and 100% (5/5) in cats. A total of 10 STs and 10 RTs were identified in this study. The percentages of ST42 (RT106) and ST2 (RT014/LW01) among 16 toxic strains were 41.7 and 12.5%, respectively. However, ST3 (RT001), ST1 (RT027), ST133 (LW04), and ST-UN (LW04) had only one strain. ST42 (RT106) was the most common genotype and RT027 strain was first isolated in China from pets. Antimicrobial susceptibility test showed that isolates were extremely sensitive to vancomycin and metronidazole but were resistant to erythromycin and ciprofloxacin. The drug resistant rates to clindamycin, levofloxacin, moxifloxacin and meropenem were 62.5, 20.8, 16.7, and 8.3%, respectively. In conclusion, C. difficile was quietly prevalent in dogs and cats in Lanzhou city with RT106 and RT014 as the main ribotypes. The CDI in pets should be paying more attention and further studies are needed.
Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection
Authors: Navarro Paula Fátima , Fernández-Barredo Salceda , Gil Laura
Published: November 2022

Introduction

The study of early markers to detect kidney malfunction has increased in recent years since serum markers, such as creatinine increase when there is a 75% loss of renal mass. Urinary capillary electrophoresis (UCE) is an available laboratory technique that provides an easily interpretable electrophoretic pattern. This pattern in our study has been divided into five fractions as it is done in serum: fraction 1 migrating in the albumin zone, fraction 2 in the alpha1-globulins zone, fraction 3 in the alpha2-globulins zone, fraction 4 in the beta-globulins zone, and fraction 5 in the gamma globulins zone. UCE can be useful in the early diagnosis of renal disease.

Material and methods

In this study, UCE was performed in dogs with azotemia and proteinuria due to chronic kidney disease (CKD) not related to Leishmania infantum (L. infantum) infection (G1, n = 11) and dogs with CKD related to L. infantum infection (G2, n = 17) and compared with reference intervals from healthy dogs (G0, n = 123), with the aim of comparing their phoretograms and assessing changes in the fractions of the phoretograms based on the health status of individuals.

Results

Fraction 2 was statistically augmented in dogs with CKD (G1) when compared with the healthy population (G0) and dogs infected by L. infantum (G2). Fraction 3 was statistically increased in dogs with CKD (G1) and dogs infected by L. infantum (G2) compared with G0. Fraction 4 was found to be statistically decreased in dogs with CKD (G1) and dogs infected by L. infantum (G2) compared with G0. Fraction 5 was statistically higher in dogs with L. infantum (G2) compared with G0 and dogs with CKD (G1). No statistical relationship was found between the protein to creatinine ratio and different fractions from the urinary phoretogram in the study population. No statistical relationship was found between serum and urine fractions in the study population.

Discussion

The results of the present study suggest that UCE is a promising non-invasive technique that might be used as a part of the diagnostic and follow-up in dogs with kidney disease due to different pathologies.
Performance Assessment of a Low-Cost Miniature Electrohydrostatic Actuator
Authors: Deibert Brendan , Wiens Travis
Published: November 2022

Low-cost small-scale (<100 W) electrohydrostatic actuators (EHAs) are not available on the market, largely due to a lack of suitable components. Utilizing plastic 3D printing, a novel inverse shuttle valve has been produced which, when assembled with emerging small-scale hydraulic pumps and cylinders from the radio-controlled hobby industry, forms a low-cost and high-performance miniature EHA. This paper presents experimental test results that characterize such a system and highlight its steady, dynamic, and thermal performance capabilities. The results indicate that the constructed EHA has good hydraulic efficiency downstream of the pump and good dynamic response but is limited by the efficiency of the pump and the associated heat generated from the pump’s losses. The findings presented in this paper validate the use of a 3D printed plastic inverse shuttle valve in the construction of a low-cost miniature EHA system.
Analytical Modeling of Density and Young’s Modulus Identification of Adsorbate with Microcantilever Resonator
Authors: Yang Yue , Tian Yanling , Liu Xianping , Song Yumeng , Tang Hui
Published: November 2022

Density and Young’s modulus are critical parameters in biological research, which can be used to characterize molecules, cells, or tissues in the diagnosis of severe diseases. Microcantilever resonators are ideal tools to measure the physical parameters of small objects at the micro/nanoscale. In this study, a mathematical model was built based on the Rayleigh–Ritz method with the consideration of the first five-order bending natural frequencies. The mathematical model can be used to detect the density and Young’s modulus of an adsorbate on a cantilever resonator with a single measurement. The influence of different order natural frequencies and the adsorbate position on the measurement accuracy and reliability was analyzed. This study revealed that the frequency pairs and the relative position of the adsorbate on the cantilever are two important factors that affect the accuracy and reliability of the measurement. Choosing appropriate frequency pairs can help to improve the accuracy and reliability of measurement. Finally, the results of finite element analysis verified the proposed method.
Impact of Consumer Awareness and Behavior on Business Exits in the Hospitality, Tourism, Entertainment, and Culture Industries under the COVID-19 Pandemic
Authors: Okamuro Hiroyuki , Hara Yasushi , Iwaki Yunosuke
Published: November 2022

Empirical studies on small business survival and exits focus on endogenous firms and top manager characteristics, whereas few studies consider exogenous demand shocks and local consumer awareness and behavior, which are especially important for local hospitality industries. Therefore, this study addresses this research gap by targeting the COVID-19 pandemic and anti-contagion policies as a local demand shock for service industries. We empirically investigate the causal effects of changing local consumer awareness and behavior under COVID-19 on business exits at the prefecture-industry level. Based on a panel fixed-effect estimation using a longitudinal dataset of 32 service industries in 47 prefectures over 10 months in Japan, we demonstrate that an increase in consumers’ risk aversion and sympathy for self-restraint from going out, and a decrease in going out with family members, significantly increase the exit ratio in specific service industries in the same prefecture. Moreover, we find that these effects vary by consumer type depending on factors such as gender, age, income level, and household structure.
Effect of Relationship Quality in Collaboration and Innovation of Agricultural Service Supply Chain under Omni-Channel Model
Authors: Yang Baojun , Yuan Bo , Yang Ning , Liu Yan , Jia Ruiqi , Wang Yongyan , Miao Ting , Liu Jianxu , Sriboonchitta Songsak
Published: November 2022

The goal of this study is to investigate the regulation effect of relationship quality in the process of the omni-channel (OC) model on service supply chain (SSC) collaboration of agricultural products. Furthermore, it is also to explore the intermediary effect of SSC collaboration in the process of service innovation and OC. A questionnaire was developed, research data were gathered from businesses in the agricultural SSC in western China, and an empirical study was carried out by using the AMOS multivariate statistical analysis approach after a thorough review of the literature in recent years. The study demonstrates that the OC model has a considerable impact on service innovation, SSC collaboration has an intermediary effect, and the quality of supply chain (SC) relationships has a regulation effect in the model. The results inspire academics and industry professionals to focus on SSC collaboration, improve the OC model’s administration, and promote service innovation in agricultural SC. Finally, the paper proposes suggestions to promote agricultural product development in western China in terms of enhancing SSC collaboration, OC model, and service innovation.
Influence of Nitrogen Fertilizer Rate on Yield, Grain Quality and Nitrogen Use Efficiency of Durum Wheat (Triticum durum Desf) under Algerian Semiarid Conditions
Authors: Boulelouah Nadia , Berbache Mohamed , Bedjaoui Hanane , Selama Nora , Rebouh Nazih
Published: November 2022

Nitrogen fertilizer application is conditioned closely by the amount of rainfall and its distribution. The current study aims at studying the effect of nitrogen (N) application rate on grain yield (GY), grain protein content (GPC), and nitrogen use efficiency (NUE) of durum wheat under Algerian semiarid conditions. Field trials were conducted during two contrasting and successive growing seasons (a dry year = 2016–2017 and a wet year = 2017–2018) on a local variety named Bousselam. A randomized complete block design (RCBD) was used with four replicates. Seven gradual treatments of fertilizer rate were studied: T1 = 0 unity of nitrogen (UN), T2 = 100 UN, T3 = 110 UN, T4 = 120 UN, T5 = 130 UN, T6 = 140 UN, and T7 = 150 UN. Results showed a significant difference between the dry and wet years. Only the GPC was higher in the dry year compared to the wet year having a difference of 2.94%. However, all other studied parameters were higher in the wet year, which resulted in a yield difference of 4.38 t ha−1. In addition, a significant effect of N rate on GY, thousand grain weight (TGW), and GPC was observed. A considerable difference between 120 UN and 150 UN was not noted in both years of study. Furthermore, the agronomic efficiency (AE) increased significantly with rainfall amount achieving a difference of 16.2 kg·kgN−1 between years. Finally, the results showed that using a high N amount led to a decrease in AE. Based on GY, apparent recovery efficiency (ARE), agronomic efficiency (AE), and marginal rate return (MRR) recorded in both years, the nitrogen application rate of 120 UN is recommended to be applied to wheat crops in Algerian semiarid conditions.
Application of Spent Sun Mushroom Substrate in Substitution of Synthetic Fertilizers at Maize Topdressing
Authors: Alves Lucas da Silva , Caitano Cinthia Elen Cardoso , Ferrari Samuel , Vieira Júnior Wagner Gonçalves , Heinrichs Reges , de Almeida Moreira Bruno Rafael , Pardo-Giménez Arturo , Zied Diego Cunha
Published: November 2022

Synthetic fertilization can increase maize yields, but also cause environmental impacts, as well as increasing production costs and food security risks. Sun mushroom (Agaricus subrufescens) is an important Brazilian fungus used to generate large amounts of spent mushroom substrate. This residue can be used for maize fertilization, but little is known about its ideal application rates to reduce maize dependence on synthetic fertilizers. Therefore, this study aimed to evaluate the agronomic performance of a maize crop under different combinations of synthetic fertilizers and two different spent mushroom substrate doses. The experiment was carried out in pots and evaluated maize germinate and biometric parameters, as well as soil and leaf chemical characteristics. The results showed that residue application increased maize germination and Emergence Speed Index. Regarding the maize biometric parameters, height, stem diameter, shoot fresh and dry masses, and leaf area were superior for residue with synthetic fertilization at sowing only at higher doses. Moreover, residue with synthetic fertilization at sowing proved to be more relevant for maize growth according to canonical discriminant analysis. In terms of nutrients, the use of spent mushroom substrate increased significantly leaf P, K, and S levels and mainly K content in the soil, justifying non-application at maize topdressing.
Effects of Dietary Inclusion of Tannin-Rich Sericea Lespedeza Hay on Relationships among Linear Body Measurements, Body Condition Score, Body Mass Indexes, and Performance of Growing Alpine Doelings and Katahdin Ewe Lambs
Authors: Wang Wei , Patra Amlan Kumar , Puchala Ryszard , Ribeiro Luana , Gipson Terry Allen , Goetsch Arthur Louis
Published: November 2022

The objective of this study was to assess the effects of the dietary level of a condensed tannin-rich forage on linear measures, body condition score (BCS), body mass indexes (BMI), and performance and relationships among these variables in growing dairy goats and hair sheep raised for meat. An experiment with a 2 × 3 factorial treatment arrangement was conducted, with two species and three diets. Diets were 25% concentrate and 75% forage, which were alfalfa hay, condensed tannin-containing Sericea lespedeza hay, and a 1:1 mixture of both hay sources. Twenty-four Alpine (ALP) doelings and 24 Katahdin (KAT) ewe lambs were used in the 173-day study, consisting of four measurement periods. Variables included BCS, linear measures, BMI, and performance variables such as average daily gain (ADG) and dry matter intake (DMI, g/day). Linear measures were length from the shoulder point to pin (Pin) and hook (Hook) bones, height at the withers (Wither), circumference from heart girth (Heart), and width at the hook bones (Rump). Different BMI were based on the Wither, Hook, Pin, Heart, and various combinations. Heart, Rump, and all BMI were affected by species, whereas linear measures and BMI based on Wither and the combination of height and length measures were influenced by diet. There were positive (p < 0.05) correlation coefficients (r) between BCS and body weight (BW) and linear measures as well as BMI for both species, and correlations were greater for KAT than for ALP. Body condition score was correlated with BW, ADG, and DMI for KAT (p < 0.05). For ALP, the correlation between BCS and BW (p < 0.015) was much lower than for KAT (0.49 vs. 0.91), and there were only tendencies for relationships between BCS and ADG and DMI (p < 0.10). Body weight and ADG were positively correlated (p < 0.05) with all BMI for both species, and most correlations were greater for KAT than for ALP. Correlations involving BMI based on combinations of two linear measures with BW and ADG were in most instances greater than for BMI calculated from one or three linear measures. For ALP, DMI was related to BMI based on Wither and Pin (r = 0.43), Heart and Hook (r = 0.44), and Heart and Pin (r = 0.61), whereas for KAT, correlations were similar (0.72–0.75) for each of the four BMI based on two linear measures (i.e., Wither and Hook, Wither and Pin, Heart and Hook, and Heart and Pin). Therefore, it appears that each of these four BMI, preferably based on Heart and length, could be appropriate for better livestock management over BCS and in predicting animal performance.
Temperature Incubation Influences Gonadal Gene Expression during Leopard Gecko Development
Authors: Pallotta Maria Michela , Fogliano Chiara , Carotenuto Rosa
Published: November 2022

During development, sexual differentiation results in physiological, anatomical and metabolic differences that implicate not only the gonads but also other body structures. Sex in Leopard geckos is determined by egg incubation temperature. Based on the premise that the developmental decision of gender does not depend on a single gene, we performed an analysis on E. macularius to gain insights into the genes that may be involved in gonads’ sexual differentiation during the thermosensitive period. All the genes were identified as differentially expressed at stage 30 during the labile phase of sex differentiation. In this way, the expression of genes known to be involved in gonadal sexual differentiation, such as WNT4, SOX9, DMRT1, Erα, Erβ, GnRH, P450 aromatase, PRLand PRL-R, was investigated. Other genes putatively involved in sex differentiation were sought by differential display. Our findings indicate that embryo exposure to a sex-determining temperature induces differential expression of several genes that are involved not only in gonadal differentiation, but also in several biological pathways (ALDOC, FREM1, BBIP1, CA5A, NADH5, L1 non-LTR retrotransposons, PKM). Our data perfectly fit within the new studies conducted in developmental biology, which indicate that in the developing embryo, in addition to gonadal differentiation, sex-specific tissue and metabolic polarization take place in all organisms.