bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysing the Effect of Substrate Properties on Building Envelope Thermal Performance in Various Climates
Authors: Zingre Kishor T. , Kumar D. E. V. S. Kiran , Wan Man Pun
Published: October 2020

Existing regulations on the thermal efficiency of building envelope assemblies are based on the steady state thermal properties of substrate materials. Heat transfer mechanisms of passive heat curbing methods such as phase change materials and cool materials, which are dynamic in nature, are currently not being accounted for. The effectiveness of thermo-physical and solar radiation properties of building materials (i.e., solid homogeneous layers without air gap) in reducing the heat gain into a building in a hot climate could be well understood with the equivalent thermal resistance (Req) concept. A simple and easy-to-use mathematical derivation (i.e., to estimate the instantaneous heat flux across an envelope assembly) is proposed in this paper to understand the mechanism of equivalent R-value (i.e., reciprocal of thermal transmittance, U-value) due to solar radiation properties of passive substrate materials. The model is validated against field experiments carried out at two apartment units of a residential building. The Req due to high outer surface solar radiation properties (i.e., by applying a cool coating) is dynamic as it varies with the weather conditions. The effect of a substrate material’s solar radiation and thermo-physical properties on the overall roof thermal performance is investigated using the Req model for four cooling dominated climates around the globe, having different diurnal conditions and sky temperatures. Increasing the outer surface’s solar reflectance (from 10% to 80%) reduces net heat gain through the flat roof during both daytime and nighttime. In contrast, adding only thermal resistance (from 5 mm to 75 mm thick polyurethane) or volumetric heat capacity (by adding 5 mm thick phase change material) to the building envelope brings down heat gain during the day, but not in the night. Thermal insulation is found to be the second effective property, followed by thermal mass irrespective of different diurnal conditions and sky temperatures across the climates.
Environmental Impacts of Integrated Photovoltaic Modules in Light Utility Electric Vehicles
Authors: Kanz Olga , Reinders Angèle , May Johanna , Ding Kaining
Published: October 2020

This paper presents a life cycle assessment (LCA) of photovoltaic (PV) solar modules which have been integrated into electric vehicle applications, also called vehicle integrated photovoltaics (VIPV). The LCA was executed by means of GaBi LCA software with Ecoinvent v2.2 as a background database, with a focus on the global warming potential (GWP). A light utility electric vehicle (LUV) named StreetScooter Work L, with a PV array of 930 Wp, was analyzed for the location of Cologne, Germany. An operation time of 8 years and an average shadowing factor of 30% were assumed. The functional unit of this LCA is 1 kWh of generated PV electricity on-board, for which an emission factor of 0.357 kg CO2-eq/kWh was calculated, whereas the average grid emissions would be 0.435 kg CO2-eq/kWh. Hence, charging by PV power hence causes lower emissions than charging an EV by the grid. The study further shows how changes in the shadowing factor, operation time, and other aspects affect vehicle’s emissions. The ecological benefit of charging by PV modules as compared to grid charging is negated when the shadowing factor exceeds 40% and hence exceeds emissions of 0.435 kg CO2-eq/kWh. However, if the operation time of a vehicle with integrated PV is prolonged to 12 years, emissions of the functional unit go down to 0.221 kg CO2-eq/kWh. It is relevant to point out that the outcomes of the LCA study strongly depend on the location of use of the vehicle, the annual irradiation, and the carbon footprint of the grid on that location.
The Induction of Adventitious Roots Regeneration before Transplanting Rootless Ficus elastica Heritage Tree
Authors: Li Nelson , Tu Pei-Chun , Lo Kuo-Chin , Chang Yu-Sen
Published: October 2020

Heritage trees carry both botanical and historical value for a city’s resilience and sustainability and hence are precious and unique. Their transplant is costly and very rare due to tremendous cost and 100% survival requirement by law. Rootless transplant is even more detrimental to the heritage tree due to removal of roots infected by brown root rot (BRR) before transplanting. This study examined the adventitious roots (AR) induction ability of the Ficus elastica Roxb. heritage tree infected with BRR. The experimental design considered three factors: root diameter (RD), wounding method (WM), and auxin solution on aerial roots under fractional factorial experiment in completely randomized design (CRD). There were four RD groups: RDI (RD < 2 cm), RDII (2 ≤ RD ≤ 4.3 cm), RDIII (4.3 < RD ≤ 22), and RDIV (RD > 22); three WMs: cutting off (CF), girdling (GD), and rectangular shape peeling (RP) of aerial roots; and three auxin solutions: 2000 mg·L−1 IBA(Indole-3-butyric acid) (2B), 2000 mg·L−1 IBA + 2000 mg·L−1 NAA(1-Naphthaleneacetic acid) (2NB), and 4000 mg·L−1 IBA (4B) plus water as control (C). The number of rooting wounds, number of roots, and the mean length of the three longest adventitious roots in each wound were recorded to evaluate the AR rooting performance. Twenty four treatment combinations including 328 wounds were tested. The results showed that rooting ability was significantly correlated with RD and WM. Smaller RDs had better rooting and declined with increased RDs. CF had the best rooting followed by GD and then RP. Auxin solution did not significantly affect the rooting ability. It may be due to the abundant endogenous auxin in the heritage tree, which mitigated the effect of exogenous auxin for AR induction. We conclude that cutting off small-diameter aerial roots is the best approach to induce ARs from rootless F. elastica heritage trees to enhance transplantation success.
Growth Rates of Lymantria dispar Larvae and Quercus robur Seedlings at Elevated CO2 Concentration and Phytophthora plurivora Infection
Authors: Milanović Slobodan , Milenković Ivan , Dobrosavljević Jovan , Popović Marija , Solla Alejandro , Tomšovský Michal , Jankovský Libor
Published: October 2020

Interactions between plants, insects and pathogens are complex and not sufficiently understood in the context of climate change. In this study, the impact of a root pathogen on a leaf-eating insect hosted by a tree species at elevated CO2 concentration is reported for the first time. The combined and isolated effects of CO2 and infection by the root pathogen Phytophthora plurivora on English oak (Quercus robur) seedlings were used to assess growth rates of plants and of gypsy moth (Lymantria dispar) larvae. For this purpose, two Q. robur provenances (Belgrade and Sombor) were used. At ambient CO2 concentration, the relative growth rates of larvae consuming leaves of plants infected by P. plurivora was higher than those consuming non-infected plants. However, at elevated CO2 concentration (1000 ppm) higher relative growth rates were detected in the larvae consuming the leaves of non-infected plants. At ambient CO2 concentration, lower growth rates were recorded in L. dispar larvae hosted in Q. robur from Belgrade in comparison to larvae hosted in Q. robur from Sombor. However, at elevated CO2 concentration, similar growth rates irrespective of the provenance were observed. Defoliation by the gypsy moth did not influence the growth of plants while P. plurivora infection significantly reduced tree height in seedlings from Belgrade. The results confirm that a rise of CO2 concentration in the atmosphere modifies the existing interactions between P. plurivora, Q. robur, and L. dispar. Moreover, the influence of the tree provenances on both herbivore and plant performance at elevated CO2 concentrations suggests a potential for increasing forest resilience through breeding.
Incidence of Post-Traumatic Stress Disorder after Coronavirus Disease
Authors: Chang Min Cheol , Park Donghwi
Published: October 2020

Background: The coronavirus disease (COVID-19) emerged from China and rapidly spread to many other countries. In this study, we investigated the prevalence of post-traumatic stress disorder (PTSD) among patients with COVID-19 who were treated and discharged from a university hospital in Daegu, Korea. Methods: In total, 64 patients who were diagnosed with COVID-19 and then hospitalized, treated and discharged from the university hospital between February and April 2020 participated in our study. We conducted telephone interviews with the participants and evaluated the presence of PTSD using the Post-Traumatic Stress Disorder Checklist-5 (PCL-5) based on the Diagnostic and Statistical Manual of Mental Disorders (DSM-5; score range: 0–80). If a score of ≥33 was obtained, then a diagnosis of PTSD was made. We analyzed the association between PTSD and demographic and clinical characteristics using the Mann–Whitney U and chi-square tests. Results: In total, 13 patients had a PCL-5 score of ≥33, which indicated that 20.3% (n = 64) of the patients had PTSD. No significant differences were observed in demographic characteristics, including, sex, age, hospitalization time and duration after discharge, between patients with PTSD and those without PTSD. Conclusions: The prevalence rate of PTSD was 20.3% in patients with COVID-19 who had been hospitalized, treated and discharged. Accordingly, clinicians should be aware of the high possibility of PTSD among COVID-19 patients. Mental health interventions supporting the mental health of patients should be provided to affected patients.
Bullying and Harassment in Downsized Workplaces: What Can We Learn from the 2008 Icelandic Economic Collapse?
Authors: Sigursteinsdottir Hjordis , Rafnsdottir Gudbjorg Linda , Jonsdottir Gudbjorg Andrea
Published: October 2020

Research shows that bullying is a significant workplace issue. A previous study showed increased sickness-related absences among municipality employees during the Icelandic economic crisis in 2008. This led to the following research questions: has bullying and/or harassment increased between the time points of the study up to seven years after the crisis? Did bullying and/or harassment change depending on downsizing? Are quantitative job demands, role conflicts and social support connected to bullying and/or harassment at work and if so, how? The study is based on a four-wave longitudinal balanced panel dataset consisting of those who work within the education and care services operated by Icelandic municipalities. It was seen that bullying and harassment had increased between the time points of the study. Furthermore, employees in downsized workplaces, workplaces with higher quantitative job demands, more role conflicts and less support were more likely to experience bullying and/or harassment than employees in other workplaces. Since the effects may prevail for several years, the study demonstrates that the consequences of downsizing need to be carefully considered and that managers must be supported in that role. As economic crises tend to occur periodically, presently due to COVID-19, the knowledge is both of theoretical and practical importance.
Bad Air Can Also Kill: Residential Indoor Air Quality and Pollutant Exposure Risk during the COVID-19 Crisis
Authors: Domínguez-Amarillo Samuel , Fernández-Agüera Jesica , Cesteros-García Sonia , González-Lezcano Roberto Alonso
Published: October 2020

During the first outbreak of the SARS-CoV-2 pandemic the population, focusing primarily on the risk of infection, was generally inattentive to the quality of indoor air. Spain, and the city of Madrid in particular, were among the world’s coronavirus hotspots. The country’s entire population was subject to a 24/7 lockdown for 45 days. This paper describes a comparative longitudinal survey of air quality in four types of housing in the city of Madrid before and during lockdown. The paper analysed indoor temperatures and variations in CO2, 2.5 μm particulate matter (PM2.5) and total volatile organic compound (TVOC) concentrations before and during lockdown. The mean daily outdoor PM2.5 concentration declined from 11.04 µg/m3 before to 7.10 µg/m3 during lockdown. Before lockdown the NO2 concentration values scored as ‘very good’ 46% of the time, compared to 90.9% during that period. Although the city’s outdoor air quality improved, during lockdown the population’s exposure to indoor pollutants was generally more acute and prolonged. Due primarily to concern over domestic energy savings, the lack of suitable ventilation and more intensive use of cleaning products and disinfectants during the covid-19 crisis, indoor pollutant levels were typically higher than compatible with healthy environments. Mean daily PM2.5 concentration rose by approximately 12% and mean TVOC concentration by 37% to 559%. The paper also puts forward a series of recommendations to improve indoor domestic environments in future pandemics and spells out urgent action to be taken around indoor air quality (IAQ) in the event of total or partial quarantining to protect residents from respiratory ailments and concomitantly enhanced susceptibility to SARS-CoV-2, as identified by international medical research.
Racial/Ethnic Differences in Health Behaviors and Its Roles on Depressive Symptoms among Young Female Adults
Authors: Lee Jaewon , Seon Jisuk
Published: October 2020

This study explores the role of health behaviors on depressive symptoms across young adult females and differences in the relationship across race/ethnicity. The data come from the National Longitudinal Survey of Youth 1979 Child and Young Adult. Seven hundred and seven non-Hispanic White females, 592 African American females, and 349 Hispanic females were selected. Multiple linear regression and logistic regression analyses were conducted. African American and Hispanic females were more likely to eat fast food than non-Hispanic Whites. African Americans reported that they ate fruit less frequently in comparison with non-Hispanic Whites. Fruit intake was related to lower levels of depressive symptoms. Hispanics moderated the association between fruit intake and depressive symptoms. Females should be encouraged to eat more fruit during young adulthood in order to diminish the likelihood of depressive symptoms. In addition, strategies for promoting healthy behaviors should consider the varied effects of race/ethnicity on depressive symptoms among young female adults.
Classical Biomarker and Quantitative Extended Diamondoid Analysis Fingerprints for Crude Oils from Deepwater Developments in Block 17, Lower Congo Basin, Angola
Authors: Boente Carlos , Márquez Gonzalo , Marín Patricia , Romero Emilio , Rodrigues Cristina , Guzmán Marco Antonio
Published: October 2020

The organic geochemistry of six oil samples from the offshore Block 17 (Lower Congo Basin, northwestern Angola) was studied by a combination of classical biomarker and extended diamondoid analyses to elucidate source rock facies, the extent of biodegradation, and thermal maturity. Based on molecular data, oils are interpreted as depicting a mixture of two pulses of hydrocarbon generation probably from the Bucomazi and Malembo formations. Geochemical results also gave evidence of mixing of a lacustrine siliciclastic-sourced oil charge and a second more terrestrially derived oil type in the samples analyzed. A single genetic oil family was identified through hierarchical cluster analysis; however, two groups of oils were identified on the basis of their biodegradation levels using the Peters/Moldowan scale. Lower and upper Malembo oils have a slight depletion and a notable absence of n-alkanes, suggesting PM levels of 1 and 2, respectively. Most molecular maturity parameters of the oil samples suggest a maturity level equivalent to the onset of the peak of the oil generative window.
The Predictors of Driving Cessation among Older Drivers in Korea
Authors: Moon SeolHwa , Park Kyongok
Published: October 2020

Background: As the elderly population and the number of older drivers grow, public safety concerns about traffic accidents involving older drivers are increasing. Approaches to reduce traffic accidents involving older drivers without limiting their mobility are needed. This study aimed to investigate the driving cessation (DC) rate among older Korean adults and predictors of DC based on the comprehensive mobility framework. Method: In this cross-sectional study, data from 2970 to 10,062 older adults over 65 years old from the 2017 National Survey of Elderly People were analyzed in April 2020. Multivariate logistic regression analyses were conducted to identify the predictors of DC. Results: Residential area, an environmental factor, was a strong predictor of DC (Odds Ratio (OR) 2.21, 95% Confidential Interval (CI) 1.86–2.62). Older drivers living in an area with a metro system were 2.21 more likely to stop driving than those living in an area without a metro system. Other demographic, financial, psychosocial, physical, and cognitive variables also predicted DC. Conclusion: Environmental factors were strong predictors of older adults’ DC. Therefore, political and environmental support, such as the provision of accessible public transportation, is essential to increase the DC rate among older adults to increase public safety without decreasing their mobility.
The Relationship between Risk Perception of Cell Phones and Objective Knowledge of EMF in Korea
Authors: Seo Myung-Soon , Choi Jae-Wook , Kim Kyung-Hee , Choi Hyung-Do
Published: October 2020

This study examines differences between the level of objective knowledge regarding radio-frequency electromagnetic fields (RF-EMF) and risk perception of cell phones in Korea. We also investigate the extent to which socio-demographic factors, perceived EMF exposure, objective knowledge regarding EMF, and psychological factors influence the risk perception of cell phones using hierarchical multiple regression. All 3393 study subjects completed a survey measuring the degree of risk perception of EMF. They were sampled in accordance with representative proportions of sex, age group, and region of residence as shown in the 2019 Resident Registration Population Statistics reported by Korea. The variables that have the most influence on risk perception of cell phones can be induced from the beta values for each variable: The subjective factor, perceived level of exposure to EMF (β = 0.253), was more strongly related to risk perception of cell phones than level of knowledge regarding EMF, an objective factor in this study. Of the psychological factors, Dreadfulness (β = 0.331), Personal knowledge (β = −174), and Familiarity (β = −089) influenced risk perceptions of cell phones; Controllability did not. On the risk cognition map, people though that it was easy to control risk related to Cell phone use in daily life, while risk related to High technology was uncontrollable.
Emergency Department Overcrowding: A Retrospective Spatial Analysis and the Geocoding of Accesses. A Pilot Study in Rome
Authors: Pesaresi Cristiano , Migliara Giuseppe , Pavia Davide , De Vito Corrado
Published: October 2020

The overcrowding of first aid facilities creates considerable hardship and problems which have repercussions on patients’ wellbeing, the time needed for a diagnosis, and on the quality of the assistance. The basic objective of this contribution, based on the data collected by the Hospital Policlinico Umberto I in Rome (Lazio region, Italy), is to carry out a territorial screening of the municipality using GIS applications and spatial analyses aimed at reducing—in terms of triage—code white (inappropriate) attendances, after having identified the areas of greatest provenance of improperly used emergency room access. Working in a GIS environment and using functions for geocoding, we have tested an experimental model aimed at giving a close-up geographical-sanitary look at the situation: recognizing the territorial sectors in Rome which contribute to amplifying the Policlinico Umberto I emergency room overcrowding; leading up to an improvement of the situation; promoting greater awareness and knowledge of the services available on the territory, a closer relationship between patient and regular doctor (general practitioner, GP) or Local Healthcare Unit and a more efficient functioning of the emergency room. In particular, we have elaborated a “source” map from which derive all the others and it is a dot map on which all the codes white have been geolocalized on a satellite image through geocoding. We have produced three sets made up of three digital cartographic elaborations each, constructed on the census sections, the census areas and the sub-municipal areas, according to data aggregation, for absolute and relative values, and using different templates. Finally, following the same methodology and steps, we elaborated another dot map about all the codes red to provide another kind of information and input for social utility. In the near future, this system could be tested on a platform that spatially analyzes the emergency department (ED) accesses in near-real-time in order to facilitate the identification of critical territorial issues and intervene in a shorter time to regulate the influx of patients to the ED.
The Fate of Transplanted Olfactory Progenitors Is Conditioned by the Cell Phenotypes of the Receiver Brain Tissue in Cocultures
Authors: Grégory Pourié , Nassila Akchiche , Jean-Louis Millot , Jean-Louis Guéant , Jean-Luc Daval , Carine Bossenmeyer-Pourié
Published: October 2020

Among the numerous candidates for cell therapy of the central nervous system (CNS), olfactory progenitors (OPs) represent an interesting alternative because they are free of ethical concerns, are easy to collect, and allow autologous transplantation. In the present study, we focused on the optimization of neuron production and maturation. It is known that plated OPs respond to various trophic factors, and we also showed that the use of Nerve Growth Factor (NGF) allowed switching from a 60/40 neuron/glia ratio to an 80/20 one. Nevertheless, in order to focus on the integration of OPs in mature neural circuits, we cocultured OPs in primary cultures obtained from the cortex and hippocampus of newborn mice. When dissociated OPs were plated, they differentiated into both glial and neuronal phenotypes, but we obtained a 1.5-fold higher viability in cortex/OP cocultures than in hippocampus/OP ones. The fate of OPs in cocultures was characterized with different markers such as BrdU, Map-2, and Synapsin, indicating a healthy integration. These results suggest that the integration of transplanted OPs might by affected by trophic factors and the environmental conditions/cell phenotypes of the host tissue. Thus, a model of coculture could provide useful information on key cell events for the use of progenitors in cell therapy.
Risk Factors and Mouse Models of Abdominal Aortic Aneurysm Rupture
Authors: Murali Krishna Smriti , Morton Susan K. , Li Jiaze , Golledge Jonathan
Published: October 2020

Abdominal aortic aneurysm (AAA) rupture is an important cause of death in older adults. In clinical practice, the most established predictor of AAA rupture is maximum AAA diameter. Aortic diameter is commonly used to assess AAA severity in mouse models studies. AAA rupture occurs when the stress (force per unit area) on the aneurysm wall exceeds wall strength. Previous research suggests that aortic wall structure and strength, biomechanical forces on the aorta and cellular and proteolytic composition of the AAA wall influence the risk of AAA rupture. Mouse models offer an opportunity to study the association of these factors with AAA rupture in a way not currently possible in patients. Such studies could provide data to support the use of novel surrogate markers of AAA rupture in patients. In this review, the currently available mouse models of AAA and their relevance to the study of AAA rupture are discussed. The review highlights the limitations of mouse models and suggests novel approaches that could be incorporated in future experimental AAA studies to generate clinically relevant results.
The Role of Saccharides in the Mechanisms of Pathogenicity of Fusarium oxysporum f. sp. lupini in Yellow Lupine (Lupinus luteus L.)
Authors: Formela-Luboińska Magda , Remlein-Starosta Dorota , Waśkiewicz Agnieszka , Karolewski Zbigniew , Bocianowski Jan , Stępień Łukasz , Labudda Mateusz , Jeandet Philippe , Morkunas Iwona
Published: October 2020

The primary aim of this study was to determine the relationship between soluble sugar levels (sucrose, glucose, or fructose) in yellow lupine embryo axes and the pathogenicity of the hemibiotrophic fungus Fusarium oxysporum f. sp. Schlecht lupini. The first step of this study was to determine the effect of exogenous saccharides on the growth and sporulation of F. oxysporum. The second one focused on estimating the levels of ergosterol as a fungal growth indicator in infected embryo axes cultured in vitro on sugar containing-medium or without it. The third aim of this study was to record the levels of the mycotoxin moniliformin as the most characteristic secondary metabolite of F. oxysporum in the infected embryo axes with the high sugar medium and without it. Additionally, morphometric measurements, i.e., the length and fresh weight of embryo axes, were done. The levels of ergosterol were the highest in infected embryo axes with a sugar deficit. At the same time, significant accumulation of the mycotoxin moniliformin was recorded in those tissues. Furthermore, it was found that the presence of sugars in water agar medium inhibited the sporulation of the pathogenic fungus F. oxysporum in relation to the control (sporulation of the pathogen on medium without sugar), the strongest inhibiting effect was observed in the case of glucose. Infection caused by F. oxysporum significantly limited the growth of embryo axes, but this effect was more visible on infected axes cultured under sugar deficiency than on the ones cultured with soluble sugars. The obtained results thus showed that high sugar levels may lead to reduced production of mycotoxins by F. oxysporum, limiting infection development and fusariosis.
A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells
Authors: Ayuso Miriam , Van Cruchten Steven , Van Ginneken Chris
Published: October 2020

The feed industry continuously seeks new molecules with antioxidant capacity since oxidative stress plays a key role in intestinal health. To improve screening of new antioxidants, this study aims to set up an assay to assess oxidative stress in the porcine small intestinal epithelial cell line IPEC-J2 using plate-reader-based analysis of fluorescence. Two oxidants, H2O2 and menadione, were tested at 1, 2 and 3 mM and 100, 200 and 300 µM, respectively. Trolox (2 mM) was used as the reference antioxidant and the probe CM-H2DCFDA was used to indicate intracellular oxidative stress. Cell culture, reactive oxygen species (ROS) production and assessment conditions were optimized to detect a significant ROS accumulation that could be counteracted by pre-incubation with trolox. Menadione (200 µM) reproducibly increased ROS levels, H2O2 failed to do so. Trolox significantly decreased intracellular ROS levels in menadione (200 µM)-exposed cells in a consistent way. The system was further used to screen different concentrations of the commercially available antioxidant ELIFE®. Concentrations between 100 and 200 ppm protected best against intracellular ROS accumulation. In conclusion, the combination of CM-H2DCFDA fluorescence analysis by a plate-reader, trolox as a reference antioxidant and 200 µM of menadione as a stressor agent, provides a replicable and reliable medium-throughput setup for the evaluation of intracellular oxidative stress in IPEC-J2 cells.
Beta Cell Imaging—From Pre-Clinical Validation to First in Man Testing
Authors: Demine Stephane , Schulte Michael L. , Territo Paul R. , Eizirik Decio L.
Published: October 2020

There are presently no reliable ways to quantify human pancreatic beta cell mass (BCM) in vivo, which prevents an accurate understanding of the progressive beta cell loss in diabetes or following islet transplantation. Furthermore, the lack of beta cell imaging hampers the evaluation of the impact of new drugs aiming to prevent beta cell loss or to restore BCM in diabetes. We presently discuss the potential value of BCM determination as a cornerstone for individualized therapies in diabetes, describe the presently available probes for human BCM evaluation, and discuss our approach for the discovery of novel beta cell biomarkers, based on the determination of specific splice variants present in human beta cells. This has already led to the identification of DPP6 and FXYD2γa as two promising targets for human BCM imaging, and is followed by a discussion of potential safety issues, the role for radiochemistry in the improvement of BCM imaging, and concludes with an overview of the different steps from pre-clinical validation to a first-in-man trial for novel tracers.
Identification of the Lineage Markers and Inhibition of DAB2 in In Vitro Fertilized Porcine Embryos
Authors: Oh Jong-Nam , Lee Mingyun , Choe Gyung Cheol , Lee Dong-Kyung , Choi Kwang-Hwan , Kim Seung-Hun , Jeong Jinsol , Lee Chang-Kyu
Published: October 2020

Specification of embryonic lineages is an important question in the field of early development. Numerous studies analyzed the expression patterns of the candidate transcripts and proteins in humans and mice and clearly determined the markers of each lineage. To overcome the limitations of human and mouse embryos, the expression of the marker transcripts in each cell has been investigated using in vivo embryos in pigs. In vitro produced embryos are more accessible, can be rapidly processed with low cost. Therefore, we analyzed the characteristics of lineage markers and the effects of the DAB2 gene (trophectoderm marker) in in vitro fertilized porcine embryos. We investigated the expression levels of the marker genes during embryonic stages and distribution of the marker proteins was assayed in day 7 blastocysts. Then, the shRNA vectors were injected into the fertilized embryos and the differences in the marker transcripts were analyzed. Marker transcripts showed diverse patterns of expression, and each embryonic lineage could be identified with localization of marker proteins. In DAB2-shRNA vectors injected embryos, HNF4A and PDGFRA were upregulated. DAB2 protein level was lower in shRNA-injected embryos without significant differences. Our results will contribute to understanding of the mechanisms of embryonic lineage specification in pigs.
c-Abl Tyrosine Kinase Is Regulated Downstream of the Cytoskeletal Protein Synemin in Head and Neck Squamous Cell Carcinoma Radioresistance and DNA Repair
Authors: Deville Sara Sofia , Delgadillo Silva Luis Fernando , Vehlow Anne , Cordes Nils
Published: October 2020

The intermediate filament synemin has been previously identified as novel regulator of cancer cell therapy resistance and DNA double strand break (DSB) repair. c-Abl tyrosine kinase is involved in both of these processes. Using PamGene technology, we performed a broad-spectrum kinase activity profiling in three-dimensionally, extracellular matrix grown head and neck cancer cell cultures. Upon synemin silencing, we identified 86 deactivated tyrosine kinases, including c-Abl, in irradiated HNSCC cells. Upon irradiation and synemin inhibition, c-Abl hyperphosphorylation on tyrosine (Y) 412 and threonine (T) 735 was significantly reduced, prompting us to hypothesize that c-Abl tyrosine kinase is an important signaling component of the synemin-mediated radioresistance pathway. Simultaneous targeting of synemin and c-Abl resulted in similar radiosensitization and DSB repair compared with single synemin depletion, suggesting synemin as an upstream regulator of c-Abl. Immunoprecipitation assays revealed a protein complex formation between synemin and c-Abl pre- and post-irradiation. Upon pharmacological inhibition of ATM, synemin/c-Abl protein-protein interactions were disrupted implying synemin function to depend on ATM kinase activity. Moreover, deletion of the SH2 domain of c-Abl demonstrated a decrease in interaction, indicating the dependency of the protein-protein interaction on this domain. Mechanistically, radiosensitization upon synemin knockdown seems to be associated with an impairment of DNA repair via regulation of non-homologous end joining independent of c-Abl function. Our data generated in more physiological 3D cancer cell culture models suggest c-Abl as further key determinant of radioresistance downstream of synemin.
Novel Programmed Cell Death as Therapeutic Targets in Age-Related Macular Degeneration?
Authors: Yang Ming , So Kwok-Fai , Lam Wai Ching , Lo Amy Cheuk Yin
Published: October 2020

Age-related macular degeneration (AMD) is a leading cause of severe visual loss among the elderly. AMD patients are tormented by progressive central blurring/loss of vision and have limited therapeutic options to date. Drusen accumulation causing retinal pigment epithelial (RPE) cell damage is the hallmark of AMD pathogenesis, in which oxidative stress and inflammation are the well-known molecular mechanisms. However, the underlying mechanisms of how RPE responds when exposed to drusen are still poorly understood. Programmed cell death (PCD) plays an important role in cellular responses to stress and the regulation of homeostasis and diseases. Apart from the classical apoptosis, recent studies also discovered novel PCD pathways such as pyroptosis, necroptosis, and ferroptosis, which may contribute to RPE cell death in AMD. This evidence may yield new treatment targets for AMD. In this review, we summarized and analyzed recent advances on the association between novel PCD and AMD, proposing PCD’s role as a therapeutic new target for future AMD treatment.
New Approaches to the Treatment of Chronic Hepatitis B
Authors: Alexopoulou Alexandra , Vasilieva Larisa , Karayiannis Peter
Published: October 2020

The currently recommended treatment for chronic hepatitis B virus (HBV) infection achieves only viral suppression whilst on therapy, but rarely hepatitis B surface antigen (HBsAg) loss. The ultimate therapeutic endpoint is the combination of HBsAg loss, inhibition of new hepatocyte infection, elimination of the covalently closed circular DNA (cccDNA) pool, and restoration of immune function in order to achieve virus control. This review concentrates on new antiviral drugs that target different stages of the HBV life cycle (direct acting antivirals) and others that enhance both innate and adaptive immunity against HBV (immunotherapy). Drugs that block HBV hepatocyte entry, compounds that silence or deplete the cccDNA pool, others that affect core assembly, agents that degrade RNase-H, interfering RNA molecules, and nucleic acid polymers are likely interventions in the viral life cycle. In the immunotherapy category, molecules that activate the innate immune response such as Toll-like-receptors, Retinoic acid Inducible Gene-1 (RIG-1) and stimulator of interferon genes (STING) agonists or checkpoint inhibitors, and modulation of the adaptive immunity by therapeutic vaccines, vector-based vaccines, or adoptive transfer of genetically-engineered T cells aim towards the restoration of T cell function. Future therapeutic trends would likely be a combination of one or more of the aforementioned drugs that target the viral life cycle and at least one immunomodulator.
A Review of GC-Based Analysis of Non-Invasive Biomarkers of Colorectal Cancer and Related Pathways
Authors: Monedeiro Fernanda , Monedeiro-Milanowski Maciej , Ligor Tomasz , Buszewski Bogusław
Published: October 2020

Colorectal cancer (CRC) is the third most commonly diagnosed cancer in the world. In Europe, it is the second most common cause of cancer-related deaths. With the advent of metabolomics approaches, studies regarding the investigation of metabolite profiles related to CRC have been conducted, aiming to serve as a tool for early diagnosis. In order to provide further information about the current status of this field of research, 21 studies were systematically reviewed, regarding their main findings and analytical aspects. A special focus was given to the employment of matrices obtained non-invasively and the use of gas chromatography as the analytical platform. The relationship between the reported volatile and non-volatile biomarkers and CRC-related metabolic alterations was also explored, demonstrating that many of these metabolites are connected with biochemical pathways proven to be involved in carcinogenesis. The most commonly reported CRC indicators were hydrocarbons, aldehydes, amino acids and short-chain fatty acids. These potential biomarkers can be associated with both human and bacterial pathways and the analysis based on such species has the potential to be applied in the clinical practice as a low-cost screening method.
Dislodgement Forces and Cost Effectiveness of Dressings and Securement for Peripheral Intravenous Catheters: A Randomized Controlled Trial
Authors: Schmutz Axel , Menz Lea , Schumann Stefan , Heinrich Sebastian
Published: October 2020

Objectives: Peripheral intravenous catheters (PIVC) are the most frequently used invasive devices in medicine. PIVC failure before treatment completion is a significant concern and occurs in 33–69% of patients. Partial dislodgement and accidental removal are some of the reasons for PIVC failure. The most effective dressing and securement method for preventing accidental removal remains unclear. It was the aim of this study to compare the force required to dislodge a PIVC with four commonly used dressing and securement methods. Additionally, costs were calculated. Methods: Truncated 18-gauge i.v. cannulas were attached onto the forearm of 209 volunteers using four different dressings and securements (sterile absorbent wound dressing covered by two different types of elastic polyester fleece, bordered and non-bordered polyurethane). The force during continuously stronger pulling until dislodgement was recorded. Results: The highest resistance against dislodgement forces could be observed with a sterile absorbent wound dressing covered by two incised elastic polyester fleece dressings. Commercially-manufactured bordered and non-bordered polyurethan film dressings were 20% to 75% more expensive than sterile absorbent wound dressings covered by elastic polyester fleece dressing. Conclusions: Elastic polyester fleece secured a PIVC against accidental removal by external force best, compared to commercially-manufactured bordered and non-bordered polyurethane film dressing.
The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol
Authors: Gugliandolo Agnese , Silvestro Serena , Chiricosta Luigi , Pollastro Federica , Bramanti Placido , Mazzon Emanuela
Published: October 2020

More than 120 cannabinoids were isolated from Cannabis sativa. In particular, Cannabidiol (CBD) and Cannabigerol (CBG) represent the two most studied non-psychoactive cannabinoids. However, CBG is less studied and less data are available on its biological properties and influence on synaptic transmission. On the contrary, CBD is already known to modulate brain excitatory glutamate, inhibitory γ-aminobutyric acid (GABA) and dopamine neurotransmission. In this study, using Next-Generation Sequencing (NGS) technology, we evaluated how CBG (1 or 5 µM) and CBD (1 or 5 µM) influence the transcriptome of the main neurotransmission pathways in NSC-34 motor neuron-like cells. At first, we evaluated that CBG and CBD were not cytotoxic and decreased the expression of pro-apoptotic genes. CBG and CBD are able to influence the expression of the genes involved in glutamate, GABA and dopamine signaling. Interestingly, the transcriptional changes induced by CBG were similar compared to CBD.
Designing and Fabricating Nano-Structured and Micro-Structured Radiation Shields for Protection against CBCT Exposure
Authors: Nikeghbal Kiana , Zamanian Zahra , Shahidi Shoaleh , Spagnuolo Gianrico , Soltani Parisa
Published: October 2020

Researchers have always been interested in finding new and effective materials for protection against radiation. This experimental study aimed to design and fabricate new types of nano-material and micro-material based shields against the ionizing effect of cone beam computed tomography (CBCT) X-rays. To fabricate a flexible prototype, we added dioctyl phthalate (DOP) oil to emulsion polyvinyl chloride (PVC) powder. The paste was mixed and dispersed. Then, nano- and micro-powders of WO3 and Bi2O3 were added to the paste, with the weight ratio of 20% PVC, 20% DOP, and 60% nano- and micro-metals. Using an ultrasonic mixer, the polymer matrix and metals were mixed and a paste with a thick texture was developed. The resultant paste was poured into glass molds and the molds were then heated in an oven. After cooling, the resultant sheets were selected for further experiments. A CBCT unit and dosimeter were used to evaluate the characterization and X-ray shielding properties of the fabricated prototypes. The half-value layers (HVL) for nano-WO3, micro-WO3, nano-Bi2O3, and micro-Bi2O3 were 0.0390, 0.0524, 0.0351, and 0.0374 cm, respectively. In addition, the linear attenuation coefficient (µ) for these materials were 17.77, 13.20, 19.71, and 18.5 cm−1, respectively. The findings indicate that nano-structured samples are more effective in the attenuation of X-ray energy. The nano-structured WO3 prototype was nearly 34% more efficient in attenuating radiation compared to the micro-structured WO3 prototype. This difference in nano- and micro-structured Bi2O3 prototypes was 6.5%.
Waviness of Freeform Surface Characterizations from Austenitic Stainless Steel (316L) Manufactured by 3D Printing-Selective Laser Melting (SLM) Technology
Authors: Kozior Tomasz , Bochnia Jerzy , Zmarzły Paweł , Gogolewski Damian , Mathia Thomas G.
Published: October 2020

The paper presents the results of tests of surface waviness of samples made in the powder bed fusion technology. The models were built using 316L steel-based powder with high corrosion resistance. The samples were placed on the construction platform at three different angles (0°, 45°, 90°) in XZ plane. Then, using an optical profilometer, the parameters of the geometric structure of the surface of the primary profile and the separated waviness component were measured. Analyzing the results of the test, it can be stated that the orientation of model arrangement has an impact on the quality of the technological surface texture, what has significance impact on wear processes and mechanical properties.
Magneto-Fluorescent Hybrid Sensor CaCO3-Fe3O4-AgInS2/ZnS for the Detection of Heavy Metal Ions in Aqueous Media
Authors: Kurshanov Danil , Khavlyuk Pavel , Baranov Mihail , Dubavik Aliaksei , Rybin Andrei , Fedorov Anatoly , Baranov Alexander
Published: October 2020

Heavy metal ions are not subject to biodegradation and could cause the environmental pollution of natural resources and water. Many of the heavy metals are highly toxic and dangerous to human health, even at a minimum amount. This work considered an optical method for detecting heavy metal ions using colloidal luminescent semiconductor quantum dots (QDs). Over the past decade, QDs have been used in the development of sensitive fluorescence sensors for ions of heavy metal. In this work, we combined the fluorescent properties of AgInS2/ZnS ternary QDs and the magnetism of superparamagnetic Fe3O4 nanoparticles embedded in a matrix of porous calcium carbonate microspheres for the detection of toxic ions of heavy metal: Co2+, Ni2+, and Pb2+. We demonstrate a relationship between the level of quenching of the photoluminescence of sensors under exposure to the heavy metal ions and the concentration of these ions, allowing their detection in aqueous solutions at concentrations of Co2+, Ni2+, and Pb2+ as low as ≈0.01 ppm, ≈0.1 ppm, and ≈0.01 ppm, respectively. It also has importance for application of the ability to concentrate and extract the sensor with analytes from the solution using a magnetic field.
Determination of the Elasticity Modulus of Additively Manufactured Wrist Hand Orthoses
Authors: Łukaszewski Krzysztof , Wichniarek Radosław , Górski Filip
Published: October 2020

The article describes the experimental and simulation research on the material properties of the individualized wrist orthoses produced in the additive manufacturing (AM) process by the fused filament fabrication (FFF) method. The authors produced a series of standard (normalized) samples for three-point bending from acrylonitrile butadiene styrene (ABS) filament on a low-budget 3D printer and a series of samples in the shape of a fragment of the orthosis and the entire orthosis. All types of samples were subjected to experimental tests on a universal testing machine, which allowed us to determine the modulus of elasticity of the produced materials by comparing it with finite element method (FEM) simulation models in the ABAQUS environment. The adopted research methodology allowed us to compare the material properties of the material of the entire product—wrist hand orthosis (WHO)—with the material properties of standard bending samples. The obtained values of Young’s modulus are characterized by a large discrepancy between the standard samples and the entire orthosis. On the other hand, the samples with the shape of the middle part of the orthosis were similar in the value of Young’s modulus to the results obtained during the examination of the complete orthosis.
Marshall–Olkin Length-Biased Maxwell Distribution and Its Applications
Authors: Mathew Jismi , Chesneau Christophe
Published: October 2020

It is well established that classical one-parameter distributions lack the flexibility to model the characteristics of a complex random phenomenon. This fact motivates clever generalizations of these distributions by applying various mathematical schemes. In this paper, we contribute in extending the one-parameter length-biased Maxwell distribution through the famous Marshall–Olkin scheme. We thus introduce a new two-parameter lifetime distribution called the Marshall–Olkin length-biased Maxwell distribution. We emphasize the pliancy of the main functions, strong stochastic order results and versatile moments measures, including the mean, variance, skewness and kurtosis, offering more possibilities compared to the parental length-biased Maxwell distribution. The statistical characteristics of the new model are discussed on the basis of the maximum likelihood estimation method. Applications to simulated and practical data sets are presented. In particular, for five referenced data sets, we show that the proposed model outperforms five other comparable models, also well known for their fitting skills.