bluuebunny/crossref_metadata_2025 huggingface dataset:
Hypercoagulability State Combined with Post-Treatment Hypofibrinolysis in Invasive Breast Cancer: A Seven-Year Follow-Up Evaluating Disease-Free and Overall Survival
Authors: Wrzeszcz Katarzyna , Rhone Piotr , Kwiatkowska Katarzyna , Ruszkowska-Ciastek Barbara
Published: April 2023

(1) Background: Cancer treatment, including chemotherapy, endocrine therapy, targeted therapy and radiotherapy, has been identified as an important independent risk factor for venous thromboembolism in cancer patients. The aim of the study was to evaluate the effect of adjuvant therapy on the coagulation and fibrinolysis components in invasive breast cancer. (2) Methods: Tissue factor pathway inhibitor (TFPI), tissue factor (TF), tissue plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1) antigen (concentration) and TFPI and TF activities were examined in the blood samples of 60 breast cancer patients treated by adjuvant chemotherapy, endocrine therapy, radiotherapy and immunotherapy. Blood samples were taken 24 h before primary surgery and 8 months after tumour removal surgery. (3) Results: Adjuvant therapy administrated to breast cancer patients significantly increased the concentration of plasma TF, the PAI-1 antigen and also the activity of TFPI and TF, but significantly decreased the level of the t-PA antigen. Combined chemotherapy and endocrine therapy, but not monotherapy, has an important effect on haemostatic biomarker levels. (4) Conclusions: Breast cancer patients receiving adjuvant therapy have an elevated risk of developing a hypercoagulability and hypofibrinolysis state leading to venous thromboembolism.
Gastro-Esophageal Reflux Disease and Paroxysmal Atrial Fibrillation Ablation
Authors: Floria Mariana , Iov Diana-Elena , Tanase Daniela Maria , Barboi Oana Bogdana , Baroi Genoveva Livia , Burlacu Alexandru , Grecu Mihaela , Sascau Radu Andy , Statescu Cristian , Mihai Catalina , Drug Vasile Liviu
Published: April 2023

Patients undergoing ablation for atrial fibrillation may be at increased risk of developing gastroesophageal reflux disease. We prospectively studied the presence of symptomatic gastroesophageal reflux disease in naïve patients who underwent atrial fibrillation ablation. Methods: The presence of typical symptoms suggestive of gastroesophageal reflux disease was clinically assessed by the gastroenterologist at baseline and at 3 months after ablation. In addition to that, all patients underwent upper gastrointestinal endoscopy. Results: Seventy-five patients were included in two groups: 46 patients who underwent atrial fibrillation ablation (study group) and 29 patients without ablation (control group). Patients with atrial fibrillation ablation were younger (57.76 ± 7.66 years versus 67.81 ± 8.52 years; p = 0.001), predominantly male (62.2% versus 33.3%; p = 0.030) and with higher body mass index (28.96 ± 3.12 kg/m2 versus 26.81 ± 5.19 kg/m2; p = 0.046). At three months after the ablation, in the study and control groups, there were 88.9% and 57.1% patients in sinus rhythm, respectively, (p = 0.009). Symptomatic gastroesophageal reflux disease was not more frequent in the study group (42.2% versus 61.9%; p = 0.220). There was no difference in terms of sinus rhythm prevalence in patients with versus without symptomatic gastroesophageal reflux disease (89.5% versus 88.5%; p = 0.709). Conclusion: In this small prospective study, typical symptoms suggestive of gastroesophageal reflux disease were not more frequent three months following atrial fibrillation ablation.
Phenanthrene Degradation by Photosynthetic Bacterial Consortium Dominated by Fischerella sp.
Authors: Márquez-Villa José Martín , Rodríguez-Sierra Juan Carlos , Amtanus Chequer Nayem , Cob-Calan Nubia Noemí , García-Maldonado José Quinatzín , Cadena Santiago , Hernández-Núñez Emanuel
Published: April 2023

Microbial degradation of aromatic hydrocarbons is an emerging technology, and it is well recognized for its economic methods, efficiency, and safety; however, its exploration is still scarce and greater emphasis on cyanobacteria–bacterial mutualistic interactions is needed. We evaluated and characterized the phenanthrene biodegradation capacity of consortium dominated by Fischerella sp. under holoxenic conditions with aerobic heterotrophic bacteria and their molecular identification through 16S rRNA Illumina sequencing. Results indicated that our microbial consortium can degrade up to 92% of phenanthrene in five days. Bioinformatic analyses revealed that consortium was dominated by Fischerella sp., however different members of Nostocaceae and Weeksellaceae, as well as several other bacteria, such as Chryseobacterium, and Porphyrobacter, were found to be putatively involved in the biological degradation of phenanthrene. This work contributes to a better understanding of biodegradation of phenanthrene by cyanobacteria and identify the microbial diversity related.
Tribocorrosive Aspects of Tungsten Carbide, Silicon Nitride, and Martensitic Steel under Fretting-like Conditions
Authors: Kronberger Markus , Brenner Josef
Published: April 2023

Water-based lubrication faces the common challenge of component lifetime extension which is impaired by tribocorrosion due to material surface depassivation. However, such mechanisms in a pH-neutral and low-halide electrolyte require additional understanding. A ball-on-flat configuration study of hard-phase materials in a low amplitude–high frequency sliding contact against martensitic chromium steel with contact pressures around 200 MPa is presented. Under lubrication by purified water, tungsten carbide-based metal matrix composite (MMC) with NiCr binder and silicon nitride-based ceramic (SiAlON) against DIN/EN 1.4108 steel yielded coefficients of friction above unity. Wear scar enlargement led to fretting-like conditions with adhesion becoming the fundamental wear mechanism. A tribocorrosion-induced depletion of tungsten carbide and nickel was determined for MMC. SiAlON materials suffered extreme wear under the formation of abrasive SiO2, while heat-treated DIN/EN 1.4125 steel showed lower friction and wear, but also showed signs of hydrogen embrittlement. Results from accompanying single-material corrosion experiments could not satisfactorily explain the phenomena. Including galvanic interaction and the influence of contact geometry, a new tribocorrosion model for fretting conditions is proposed. It describes an expanding anodic belt located at the inner-most crevice position of an otherwise cathodically polarized material. Low conductivity of the electrolyte is seen as a key player in this process, while the galvanic situation between two materials in contact was shown to invert when water was substituted by a wet organic phase.
D-Optimal Designs for Binary and Weighted Linear Regression Models: One Design Variable
Authors: Gündüz Necla , Torsney Bernard
Published: April 2023

D-optimality is a well-known concept in experimental design that seeks to select an optimal set of design points to estimate the unknown parameters of a statistical model with a minimum variance. In this paper, we focus on proving a conjecture made by Ford, Torsney and Wu regarding the existence of a class of D-optimal designs for binary and weighted linear regression models. Our concentration is on models with one design variable. The conjecture states that, for any given level of precision, there exists a two-level factorial design that is D-optimal for these models. To prove this conjecture, we use an intuitive approach that explores various link functions in the generalised linear model context to establish the veracity of the conjecture. We also present explicit and clear plots of various functions wherever deemed necessary and appropriate to further strengthen the proofs. Our results establish the existence of D-optimal designs for binary and weighted linear regression models with one design variable, which have important implications for the efficient design of experiments in various fields. These findings contribute to the development of optimal experimental designs for studying binary and weighted linear regression models and provide a foundation for future research in this area.
Climate Risks and Forecasting Stock Market Returns in Advanced Economies over a Century
Authors: Balcilar Mehmet , Gabauer David , Gupta Rangan , Pierdzioch Christian
Published: April 2023

In this study, we contribute to the rapidly growing climate-finance literature by shedding light on the question of whether climate risks have predictive value for stock market returns. We measure climate risks in terms of both the change in the northern hemisphere temperature anomaly and its volatility and the change in the global temperature anomaly and its volatility. We study monthly data for eight advanced countries (Canada, France, Germany, Italy, Japan, Switzerland, the United Kingdom (UK), and the United States (US)). Our sample period runs from 1916 to 2021. We control for cross-market spillovers of stock market returns and volatility as well as other risks including oil-price returns and volatility, geopolitical risks, and the gold-to-silver price ratio as a measure of investor risk aversion. Given this large array of control variables, we apply the Lasso estimator to trace out the incremental predictive value of climate risks for subsequent stock market returns. We find that climate risks do not have systematic predictive value for subsequent stock market returns. We then extend our analysis in two ways. First, we show that climate risks have short-term out-of-sample predictive value for the connectedness of stock market returns. Second, we show that climate risks have predictive power for stock market returns when we study monthly historical UK data for the sample period from 1772 to 2021.
Capital Structure and Corporate Performance: An Empirical Analysis from Central Europe
Authors: Mazanec Jaroslav
Published: April 2023

The capital structure and its indicators play a significant role in corporate finance. The aim is to estimate business performance using selected indicators describing primarily the capital structure, asset structure, or liquidity of transport companies in Central Europe. The total sample consists of almost 4000 small and medium-sized enterprises in the transport sector. This data is collected from Amadeus Bureau van Dijk. The results show that six out of ten variables are statistically significant predictors affecting business performance; two out of the six indicators are categorical variables, such as the company size classified into small and medium enterprises and the country divided into the Czech Republic, Hungary, Poland, or Slovakia. We find that Hungarian medium-sized enterprises show higher profitability than other enterprises, assuming other factors are unchanged. Finally, the results demonstrate that a high debt ratio and a high share of non-current assets in total assets have a negative impact on corporate performance in contrast to the current ratio and the share of cash and cash equivalents in total assets. In other words, liquidity and cash and its equivalents have a significant role in increasing business performance. These findings are specific because, generally, high liquidity does not positively impact performance.
Antithetic Power Transformation in Monte Carlo Simulation: Correcting Hidden Errors in the Response Variable
Authors: Ridley Dennis , Ngnepieba Pierre
Published: April 2023

Monte Carlo simulation is performed with uniformly distributed U(0,1) pseudo-random numbers. Because the numbers are generated from a mathematical formula, they will contain some serial correlation, even if very small. This serial correlation becomes embedded in the correlation structure of the response variable. The response variable becomes an asynchronous time series. This leads to hidden errors in the response variable. The purpose of this paper is to illustrate how this happens and how it can be corrected. The method is demonstrated for the case of a simple queue for which the time in the system is known exactly from theory. The paper derives the correlation between an exponential random variable and its antithetic counterpart obtained by power transform with an infinitesimal negative exponent.
Bile Acids as Inducers of Protonophore and Ionophore Permeability of Biological and Artificial Membranes
Authors: Samartsev Victor N. , Khoroshavina Ekaterina I. , Pavlova Evgeniya K. , Dubinin Mikhail V. , Semenova Alena A.
Published: April 2023

It is now generally accepted that the role of bile acids in the organism is not limited to their participation in the process of food digestion. Indeed, bile acids are signaling molecules and being amphiphilic compounds, are also capable of modifying the properties of cell membranes and their organelles. This review is devoted to the analysis of data on the interaction of bile acids with biological and artificial membranes, in particular, their protonophore and ionophore effects. The effects of bile acids were analyzed depending on their physicochemical properties: namely the structure of their molecules, indicators of the hydrophobic–hydrophilic balance, and the critical micelle concentration. Particular attention is paid to the interaction of bile acids with the powerhouse of cells, the mitochondria. It is of note that bile acids, in addition to their protonophore and ionophore actions, can also induce Ca2+-dependent nonspecific permeability of the inner mitochondrial membrane. We consider the unique action of ursodeoxycholic acid as an inducer of potassium conductivity of the inner mitochondrial membrane. We also discuss a possible relationship between this K+ ionophore action of ursodeoxycholic acid and its therapeutic effects.
A Competitive Study Using Electrospinning and Phase Inversion to Prepare Polymeric Membranes for Oil Removal
Authors: Diwan Thamer , Abudi Zaidun N. , Al-Furaiji Mustafa H. , Nijmeijer Arian
Published: April 2023

Polyacrylonitrile (PAN) is a popular polymer that can be made into membranes using various techniques, such as electrospinning and phase inversion. Electrospinning is a novel technique that produces nonwoven nanofiber-based membranes with highly tunable properties. In this research, electrospun PAN nanofiber membranes with various concentrations (10, 12, and 14% PAN/dimethylformamide (DMF)) were prepared and compared to PAN cast membranes prepared by the phase inversion technique. All of the prepared membranes were tested for oil removal in a cross-flow filtration system. A comparison between these membranes’ surface morphology, topography, wettability, and porosity was presented and analyzed. The results showed that increasing the concentration of the PAN precursor solution increases surface roughness, hydrophilicity, and porosity and, consequently, enhances the membrane performance. However, the PAN cast membranes showed a lower water flux when the precursor solution concentration increased. In general, the electrospun PAN membranes performed better in terms of water flux and oil rejection than the cast PAN membranes. The electrospun 14% PAN/DMF membrane gave a water flux of 250 LMH and a rejection of 97% compared to the cast 14% PAN/DMF membrane, which showed a water flux of 117 LMH and 94% oil rejection. This is mainly because the nanofibrous membrane showed higher porosity, higher hydrophilicity, and higher surface roughness compared to the cast PAN membranes at the same polymer concentration. The porosity of the electrospun PAN membrane was 96%, while it was 58% for the cast 14% PAN/DMF membrane.
Thin-Film Nanocomposite (TFN) Membranes for Water Treatment Applications: Characterization and Performance
Authors: Tayel Amr , Abdelaal Ahmed B. , Esawi Amal M. K. , Ramadan Adham R.
Published: April 2023

Thin-film nanocomposite (TFN) membranes have been widely investigated for water treatment applications due to their promising performance in terms of flux, salt rejection, and their antifouling properties. This review article provides an overview of the TFN membrane characterization and performance. It presents different characterization techniques that have been used to analyze these membranes and the nanofillers within them. The techniques comprise structural and elemental analysis, surface and morphology analysis, compositional analysis, and mechanical properties. Additionally, the fundamentals of membrane preparation are also presented, together with a classification of nanofillers that have been used so far. The potential of TFN membranes to address water scarcity and pollution challenges is significant. This review also lists examples of effective TFN membrane applications for water treatment. These include enhanced flux, enhanced salt rejection, antifouling, chlorine resistance, antimicrobial properties, thermal stability, and dye removal. The article concludes with a synopsis of the current status of TFN membranes and future perspectives.
Laser Cladding of Al 102 Powder on Al 4047 with Direct Energy Deposition
Authors: Kim Iksu , Kim Minsu , Kim Hyuntae , Lee Moon-Gu , Jeon Yongho
Published: April 2023

Large-scale serial production industries such as automotive and aerospace have focused on reducing weight to improve fuel economy, and many parts are manufactured from various aluminum alloys. Due to the ease of recycling of aluminum alloys, research on remanufacturing has not been very active. On the other hand, laser cladding on aluminum alloys is a surface modification and repair process that deposits a thin layer on a substrate using a laser beam whose output can be easily controlled. This makes it suitable for remanufacturing processes where thin layers of damaged parts can be easily repaired, and helps to save energy. In this study, laser cladding was performed using aluminum alloy powder (Al-102) containing Si to improve the surface hardness of Al 4047 used as automotive engine parts and repair damaged parts. Several experimental studies have been conducted regarding the effect on laser power and powder flow rate. In addition, the improvement effect through the hardness analysis of the cladded layer and the change of the microstructure through the cross-section analysis of the clad part are discussed. Finally, the experimental conditions analyzed in this study suggest a suitability for the actual remanufacturing process through multi-pass cladding through overlapping.
Effect of Wide-Spectrum Pulsed Magnetic Field on Solidification Structure of Pure Al at Constant Flow Velocity
Authors: Sun Yawei , Yao Bohuai , Zhao Zhen , Gong Yongyong
Published: April 2023

The electromagnetic force generated by a pulsed magnetic field within a metal melt leads to changes in the internal temperature and flow fields of the molten metal, thus improving the solidification of the metal structure. Using the combination of a solidification test, experimental simulation and theoretical analysis, this study simulated the distribution of both electromagnetic force and the flow field in a metal melt under wide-spectrum pulse conditions, and studied the influence of a wide-spectrum pulsed magnetic field on the solidification structure of pure aluminium with a constant flow velocity. The results of this study show that the structural refinement of the solidification of pure aluminium can be different, in spite of equal flow velocity. Furthermore, this study shows that an applied time-averaged electromagnetic force causes crystal nuclei to pass through the solid–liquid interface boundary layer and promotes the growth of crystal grains. These grains flowed with the melt flow field to achieve both refinement and homogenization of the solidified structure.
The Specific Binding and Promotion Effect of Azoles on Human Aldo-Keto Reductase 7A2
Authors: Wu Wanying , Jiang Tianqing , Lin Haihui , Chen Chao , Wang Lingling , Wen Jikai , Wu Jun , Deng Yiqun
Published: April 2023

Human AKR 7A2 broadly participates in the metabolism of a number of exogenous and endogenous compounds. Azoles are a class of clinically widely used antifungal drugs, which are usually metabolized by CYP 3A4, CYP2C19, and CYP1A1, etc. in vivo. The azole–protein interactions that human AKR7A2 participates in remain unreported. In this study, we investigated the effect of the representative azoles (miconazole, econazole, ketoconazole, fluconazole, itraconazole, voriconazole, and posaconazole) on the catalysis of human AKR7A2. The steady-state kinetics study showed that the catalytic efficiency of AKR7A2 enhanced in a dose-dependent manner in the presence of posaconazole, miconazole, fluconazole, and itraconazole, while it had no change in the presence of econazole, ketoconazole, and voriconazole. Biacore assays demonstrated that all seven azoles were able to specifically bind to AKR7A2, among which itraconazole, posaconazole, and voriconazole showed the strongest binding. Blind docking predicted that all azoles were apt to preferentially bind at the entrance of the substrate cavity of AKR7A2. Flexible docking showed that posaconazole, located at the region, can efficiently lower the binding energy of the substrate 2-CBA in the cavity compared to the case of no posaconazole. This study demonstrates that human AKR7A2 can interact with some azole drugs, and it also reveals that the enzyme activity can be regulated by some small molecules. These findings will enable a better understanding of azole–protein interactions.
Urine Metabolomic Signature of People Diagnosed with Balkan Endemic Nephropathy and Other Types of Chronic Kidney Disease Compared with Healthy Subjects in Romania
Authors: Ordodi Valentin L. , Hădărugă Nicoleta G. , Hădărugă Daniel I. , Lukinich-Gruia Alexandra T. , Mărgineanu Mihaela , Tatu Călin A. , Păunescu Virgil
Published: April 2023

Metabolomic analysis methods were employed to determine biomarkers for various chronic kidney diseases (CKDs). Modern analytical methods were developed and applied successfully to find a specific metabolomic profile in urine samples from CKD and Balkan endemic nephropathy (BEN) patients. The aim was to explore a specific metabolomic profile defined by feasible/easy-to-identify molecular markers. Urine samples were collected from patients with CKDs and BEN, and from healthy subjects from endemic and nonendemic areas in Romania. Metabolomic analysis of urine samples, extracted by the liquid-liquid extraction (LLE) method, was performed by gas chromatography-mass spectrometry (GC-MS). The statistical exploration of the results was performed through a principal component analysis (PCA) evaluation. Urine samples were statistically analyzed using a classification based on six types of metabolites. Most urinary metabolites are distributed in the center of a loading plot, meaning that these compounds do not represent significant markers for BEN. One of the most frequent and higher-concentration urinary metabolites in BEN patients was p-Cresol, a phenolic compound that implies a severe injury of the renal filtration function. The presence of p-Cresol was associated with protein-bound uremic toxins, which have specific functional groups such as indole and phenyl. In prospective studies for future investigation, prevention, and disease treatment, we suggest a larger sample size, sample extraction using other methods, and analysis using other chromatography techniques coupled with mass spectrometry, which can generate a more significant data set for statistical analysis.
A Novel Piezo Inertia Actuator Utilizing the Transverse Motion of Two Parallel Leaf-Springs
Authors: Sun Pingping , Xu Zhike , Jin Long , Zhu Xingxing
Published: April 2023

A novel linear piezo inertia actuator based on the transverse motion principle is proposed. Under the action of the transverse motion of two parallel leaf-springs, the designed piezo inertia actuator can achieve great stroke movements at a fairly high speed. The presented actuator includes a rectangle flexure hinge mechanism (RFHM) with two parallel leaf-springs, a piezo-stack, a base, and a stage. The mechanism construction and operating principle of the piezo inertia actuator are discussed, respectively. To obtain the proper geometry of the RFHM, we have used a commercial finite element program COMSOL. To investigate the output characteristics of the actuator, the relevant experiment tests including loading capacity, voltage characteristic, and frequency characteristic are adopted. The maximum movement speed and the minimum step size are 27.077 mm/s and 32.5 nm, respectively, confirming that the RFHM with two parallel leaf-springs can be used to design a piezo inertia actuator with a high speed and accuracy. Therefore, this actuator can be used in applications with fast positioning and high accuracy.
Designing Versatile Superhydrophilic Structures via an Alginate-Based Hydrophilic Plasticene
Authors: Shi Wenbo , Bai Haoyu , Tian Yaru , Wang Xinsheng , Li Zhe , Zhu Xuanbo , Tian Ye , Cao Moyuan
Published: April 2023

The rational design of superhydrophilic materials with a controllable structure is a critical component in various applications, including solar steam generation, liquid spontaneous transport, etc. The arbitrary manipulation of the 2D, 3D, and hierarchical structures of superhydrophilic substrates is highly desirable for smart liquid manipulation in both research and application fields. To design versatile superhydrophilic interfaces with various structures, here we introduce a hydrophilic plasticene that possesses high flexibility, deformability, water absorption, and crosslinking capabilities. Through a pattern-pressing process with a specific template, 2D prior fast spreading of liquids at speeds up to 600 mm/s was achieved on the superhydrophilic surface with designed channels. Additionally, 3D superhydrophilic structures can be facilely designed by combining the hydrophilic plasticene with a 3D-printed template. The assembly of 3D superhydrophilic microstructure arrays were explored, providing a promising route to facilitate the continuous and spontaneous liquid transport. The further modification of superhydrophilic 3D structures with pyrrole can promote the applications of solar steam generation. The optimal evaporation rate of an as-prepared superhydrophilic evaporator reached ~1.60 kg·m−2·h−1 with a conversion efficiency of approximately 92.96%. Overall, we envision that the hydrophilic plasticene should satisfy a wide range of requirements for superhydrophilic structures and update our understanding of superhydrophilic materials in both fabrication and application.
Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland
Authors: Santos Najara Akira Costa dos , Carvalho Vanessa Rafaela de , Souza-Neto Jayme A. , Alonso Diego Peres , Ribolla Paulo Eduardo Martins , Medeiros Jansen Fernandes , Araujo Maisa da Silva
Published: April 2023

Anopheles darlingi is a major malaria vector in the Amazon region and, like other vectors, harbors a community of microorganisms with which it shares a network of interactions. Here, we describe the diversity and bacterial composition from the midguts and salivary glands of lab-reared and field-captured An. darlingi using metagenome sequencing of the 16S rRNA gene. The libraries were built using the amplification of the region V3–V4 16S rRNA gene. The bacterial community from the salivary glands was more diverse and richer than the community from the midguts. However, the salivary glands and midguts only showed dissimilarities in beta diversity between lab-reared mosquitoes. Despite that, intra-variability was observed in the samples. Acinetobacter and Pseudomonas were dominant in the tissues of lab-reared mosquitoes. Sequences of Wolbachia and Asaia were both found in the tissue of lab-reared mosquitoes; however, only Asaia was found in field-captured An. darlingi, but in low abundance. This is the first report on the characterization of microbiota composition from the salivary glands of An. darlingi from lab-reared and field-captured individuals. This study can provide invaluable insights for future investigations regarding mosquito development and interaction between mosquito microbiota and Plasmodium sp.
Oral Administration of Lacticaseibacillus rhamnosus CRL1505 Modulates Lung Innate Immune Response against Klebsiella pneumoniae ST25
Authors: Dentice Maidana Stefania , Imamura Yoshiya , Elean Mariano , Albarracín Leonardo , Nishiyama Keita , Suda Yoshihito , Kurata Shoichiro , Jure María Ángela , Kitazawa Haruki , Villena Julio
Published: April 2023

Orally administered Lacticaseibacillus rhamnosus CRL1505 enhances respiratory immunity, providing protection against respiratory viruses and Streptococcus pneumoniae. However, the capacity of the CRL1505 strain to improve respiratory immunity against Gram-negative bacterial infections has not been evaluated before. The aim of this work was to evaluate whether the Lcb. rhamnosus CRL1505 was able to beneficially regulate the respiratory innate immune response and enhance the resistance to hypermucoviscous KPC-2-producing Klebsiella pneumoniae of the sequence type 25 (ST25). BALB/c mice were treated with the CRL1505 strain via the oral route and then nasally challenged with K. pneumoniae ST25 strains LABACER 01 or LABACER 27. Bacterial cell counts, lung injuries and the respiratory and systemic innate immune responses were evaluated after the bacterial infection. The results showed that K. pneumoniae ST25 strains increased the levels of TNF-α, IL-1β, IL-6, IFN-γ, IL-17, KC and MPC-1 in the respiratory tract and blood, as well as the numbers of BAL neutrophils and macrophages. Mice treated with Lcb. rhamnosus CRL1505 had significantly lower K. pneumoniae counts in their lungs, as well as reduced levels of inflammatory cells, cytokines and chemokines in the respiratory tract and blood when compared to infected controls. Furthermore, higher levels of the regulatory cytokines IL-10 and IL-27 were found in the respiratory tract and blood of CRL1505-treated mice than controls. These results suggest that the ability of Lcb. rhamnosus CRL1505 to help with the control of detrimental inflammation in lungs during K. pneumoniae infection would be a key feature to improve the resistance to this pathogen. Although further mechanistic studies are necessary, Lcb. rhamnosus CRL1505 can be proposed as a candidate to improve patients’ protection against hypermucoviscous KPC-2-producing strains belonging to the ST25, which is endemic in the hospitals of our region.
Effect of Essential Oils of Apiaceae, Lamiaceae, Lauraceae, Myrtaceae, and Rutaceae Family Plants on Growth, Biofilm Formation, and Quorum Sensing in Chromobacterium violaceum, Pseudomonas aeruginosa, and Enterococcus faecalis
Authors: D’Aquila Patrizia , Sena Giada , Crudo Michele , Passarino Giuseppe , Bellizzi Dina
Published: April 2023

The biological role played by essential oils extracted from aromatic plants is progressively being recognized. This study evaluated the potential antibacterial activity of ten essential oils against Chromobacterium violaceum, Pseudomonas aeruginosa, and Enterococcus faecalis by measuring their minimum inhibitory concentration. We found that essential oils exert different antimicrobial effects, with Origanum vulgare and Foeniculum vulgare demonstrating the most significant inhibitory effect on bacterial growth for C. violaceum and E. faecalis. The growth of P. aeruginosa was not affected by any essential oil concentration we used. Sub-inhibitory concentrations of essential oils reduced in C. violaceum and E. faecalis biofilm formation, violacein amount, and gelatinase activity, all of which are biomarkers of the Quorum Sensing process. These concentrations significantly affect the global methylation profiles of cytosines and adenines, thus leading to the hypothesis that the oils also exert their effects through epigenetic changes. Considering the results obtained, it is possible that essential oils can find a broad spectrum of applications in counteracting microbial contamination and preserving sterility of surfaces and foods, as well as inhibiting microbial growth of pathogens, alone or in combination with traditional antibiotics.
Oral Administration of Human-Gut-Derived Prevotella histicola Improves Sleep Architecture in Rats
Authors: Yoon Dae Wui , Baik Inkyung
Published: April 2023

(1) Background: The human gut microbiome may regulate sleep through the gut–brain axis. However, the sleep-promoting effects of gut microbiota remain unclear. (2) Methods: We obtained sleep–wake profiles from 25 rats receiving P. histicola (P. histicola group), 5 rats receiving P. stercorea (P. stercorea group), 4 rats not receiving bacteria (No administration group), and 8 rats receiving P. histicola extracellular vesicles (EV) (EV group) during the baseline, administration, and withdrawal periods. (3) Results: The P. histicola group showed increased total sleep, rapid eye movement (REM) sleep, and non-rapid eye movement (NREM) sleep time during the administration and withdrawal periods; on the last day of administration, we found significant increases of 52 min for total sleep (p < 0.01), 13 min for REM sleep (p < 0.05), and 39 min for NREM sleep (p < 0.01) over the baseline. EV administration also increased NREM sleep time on Day 3 of administration (p = 0.05). We observed a linear trend in the dose–response relationship for total sleep and NREM sleep in the P. histicola group. However, neither the no-administration group nor the P. stercorea group showed significant findings. (4) Conclusions: Oral administration of probiotic P. histicola may improve sleep and could be a potential sleep aid. Further rigorous evaluations for the safety and efficacy of P. histicola supplementation are warranted.
Evaluation of Plasma Lipocalin-2 as a Predictor of Etiology and Severity in Adult Patients with Community-Acquired Pneumonia
Authors: Boix-Palop Lucía , Vergara Andrea , Padilla Emma , Martínez Diego , Blanco Ana , Pérez Josefa , Calbo Esther , Vila Jordi , Casals-Pascual Climent
Published: April 2023

The aim of this study was to evaluate the diagnostic performance of plasma Lipocalin-2 (LCN2) concentration in adult patients with community-acquired pneumonia (CAP) to determine its etiology, severity and prognosis. A prospective observational study involving adults with CAP from November 2015 to May 2017 was conducted. Plasma LCN2 concentration was measured upon admission by a modified enzyme immunoassay coupled with chemiluminescence (Architect, Abbott Laboratories). The diagnostic performance of LCN2, C-reactive protein (CRP) and white blood cell to predict bacterial CAP was assessed. A total of 130 patients with CAP were included: 71 (54.6%) bacterial CAP, 42 (32.3%) unknown origin CAP and 17 (13.1%) viral CAP. LCN2 was higher in bacterial CAP than in non-bacterial CAP (122.0 vs. 89.7 ng/mL, respectively) (p = 0.03) with a limited ability to distinguish bacterial and non-bacterial CAP (AUROC: 0.62 [95% CI 0.52–0.72]). The LCN2 cutoff ≥ 204 ng/mL predicted the presence of pneumococcal bacteremia with an AUROC of 0.74 (sensitivity 70%, specificity 79.1%). Regarding severity, as defined by CURB-65 and PSI scores, there was a significant linear trend in the mean concentration of LCN2, exhibiting a shift from the low-risk to the intermediate-risk and high-risk group (p < 0.001 and 0.001, respectively). LCN2 concentration was associated with severity in adult patients with CAP. However, its utility as a biomarker to discriminate viral and bacterial etiology in CAP is limited.
Petrogenesis of Early Triassic Felsic Volcanic Rocks in the East Kunlun Orogen, Northern Tibet: Implications for the Paleo-Tethyan Tectonic and Crustal Evolution
Authors: Yan Dongdong , Chu Zhiqiang , Liu Zhongyuan , Wang Wei , Xiong Fuhao
Published: April 2023

The felsic volcanic rocks in orogenic belts are vital probes to understand the tectonic evolution and continental crust growth. Here, we present a comprehensive study on the zircon U–Pb geochronology, whole-rock geochemistry, and zircon Lu-Hf isotopes of Early felsic volcanic rocks from the Hongshuichuan Formation, East Kunlun Orogen, Northern Tibet, aiming to explore their petrogenesis and implications for the Paleo-Tethyan orogeny and crustal evolution. The studied felsic volcanics comprise rhyolite porphyry and rhyolite, exhibiting coeval zircon U–Pb ages of ca. 247–251 Ma. Rhyolite porphyries show metaluminous to peraluminous nature (A/CNK = 0.88–1.24) with high SiO2 contents (72.1–78.9 wt%) and moderate Mg# values (22–40), and they display enrichment of LREE with (La/Yb)N ratios of 6.02–17.9 and depletion of high field strength elements. In comparison, the rhyolites are strongly peraluminous (A/CNK = 1.09–1.74) with high SiO2 contents (71.7–74.3 wt%) and high Mg# values (43–52) and are also enriched in LREE ((La/Yb)N of 6.65–18.4) and depleted in HFSE (e.g., Nb, Ta, Ti). Combining with their different zircon Lu-Hf isotopes, i.e., enriched isotopes for the rhyolite porphyries (εHf(t) = −7.3 to −3.8) and depleted Hf isotopes for the rhyolites (ɛHf = −0.6 to +3.0), we interpret that the studied rhyolite porphyries and rhyolites were derived by partial melting of Mesoproterozoic metagreywacke sources followed by plagioclase-dominated fractional crystallization, but the latter shows the significant contribution of crust–mantle magma mixing. The mixed mantle-derived magma comes from an enriched lithospheric mantle source that had been metasomatized by subduction-related fluids. Combining with other geological evidence, we propose that the studied Early Triassic felsic volcanic rocks were formed in a subduction arc setting, and the reworking of ancient continental crust with crust–mantle magma mixing is the major mechanism of crustal evolution in the East Kunlun Paleo-Tethyan orogenic belt.
High-Pressure Experimental and DFT Structural Studies of Aurichalcite Mineral
Authors: Santamaría-Pérez David , Chuliá-Jordán Raquel , Otero-de-la-Roza Alberto , Oliva Robert , Popescu Catalin
Published: April 2023

We report on high-pressure angle-dispersive synchrotron X-ray diffraction data of a natural Zn3.78(2)Cu1.22(2)(CO3)2(OH)6 aurichalcite mineral up to 7.6 GPa and ab initio total energy calculations of the aurichalcite structure with three different Zn-Cu stoichiometries (Zn:Cu ratios = 10:0, 8:2 and 6:4). A monoclinic-to-triclinic displacive second-order phase transition was found experimentally at 3 GPa. The experimental bulk modulus of the initial P21/m aurichalcite is B0 = 66(2) GPa, with a first-pressure derivative of B0′ = 9(2). A comparison with other basic copper and zinc carbonates shows that this B0 value is considerably larger than those of malachite and azurite. This relative incompressibility occurs despite the fact that aurichalcite features a layered structure due to the number of directed hydrogen bonds between carbonate groups and the cation-centered oxygen polyhedra forming complex sheets. The existence of different bond types and polyhedral compressibilities entails a certain anisotropic compression, with axial compressibilities κa0 = 3.79(5)·10−3 GPa−1, κb0 = 5.44(9)·10−3 GPa−1 and κc0 = 4.61(9)·10−3 GPa−1. Additional density-functional theory calculations on the C2/m hydrozincite-type structure with different Zn:Cu compositional ratios shows that the aurichalcite structure is energetically more stable than the hydrozincite one for compositions of Zn:Cu = 10:0, 8:2 and 6:4 at room pressure. The pure Zn aurichalcite phase, however, was predicted to transform into hydrozincite at 18 GPa, which suggests that the experimentally observed hydrozincite structure is a metastable phase.
Recent Advances in ZnO Nanomaterial-Mediated Biological Applications and Action Mechanisms
Authors: Xie Jiani , Li Huilun , Zhang Tairan , Song Bokai , Wang Xinhui , Gu Zhanjun
Published: April 2023

In recent years, with the deepening research, metal zinc oxide (ZnO) nanomaterials have become a popular research object in the biological field, particularly in biomedicine and food safety, which is attributed to their unique physicochemical properties such as high surface area and volume ratio, luminescence effect, surface characteristics and biological activities. Herein, this review provides a detailed overview of the ZnO nanomaterial-mediated biological applications that involve anti-bacterial, anti-tumor, anti-inflammation, skin care, biological imaging and food packaging applications. Importantly, the corresponding action mechanisms of ZnO nanomaterials are pointed. Additionally, the structure and structure-dependent physicochemical properties, the common synthesis methods and the biosafety of ZnO nanoparticles are revealed in brief. Finally, the significance and future challenges of ZnO nanomaterial applications are concluded.
A Coupling Mechanism between Flicker Noise and Hot Carrier Degradations in FinFETs
Authors: Liu Minghao , Sun Zixuan , Lu Haoran , Shen Cong , Zhang Lining , Wang Runsheng , Huang Ru
Published: April 2023

A coupling mechanism between flicker noise and hot carrier degradation (HCD) is revealed in this work. Predicting the flicker noise properties of fresh and aged devices is becoming essential for circuit designs, requiring an understanding of the fundamental noise behaviors. While certain models for fresh devices have been proposed, those for aged devices have not been reported yet because of the lack of a clear mechanism. The flicker noise of aged FinFETs is characterized based on the measure-stress-measure (MSM) method and analyzed from the device physics. It is found that both the mean and deviations of the noise power spectral density increase compared with the fresh counterparts. A coupling mechanism is proposed to explain the trap time constants, leading to the trap characterizations in their energy profiles. The amplitude and number of contributing traps are also changing and are dependent on the mode of HCD and determined by the position of the induced traps. A microscopic picture is developed from the perspective of trap coupling, reproducing well the measured noise of advanced nanoscale FinFETs. The finding is important for accurate flicker noise calculations and aging-aware circuit designs.
Synthesis and Characterization of Erbium-Doped Silica Films Obtained by an Acid–Base-Catalyzed Sol–Gel Process
Authors: Abdullah Ali , Benchafia El Mostafa , Choi Daniel , Abedrabbo Sufian
Published: April 2023

Erbium-doped silica films were synthesized using a two-step sol–gel methodology that involved acid and base catalysts, with erbium concentration ranging from 0.2% to 6% and annealing temperatures varying from 500 °C to 900 °C. The photoluminescence spectra showed that the samples exhibiting efficient emission were annealed at 800 °C and 900 °C and doped with 3% and 6% erbium. The X-ray diffraction analysis revealed that the internal structure of the films was influenced by the different annealing temperatures and the doping concentrations. Samples with dominant 4f transitions were modelled. The results suggest that the proposed method is a promising approach for the synthesis of erbium-doped silica films with potential applications in optical devices.
Several Topics on Transverse Momentum-Dependent Fragmentation Functions
Authors: Chen Kai-Bao , Liu Tianbo , Song Yu-Kun , Wei Shu-Yi
Published: April 2023

The hadronization of a high-energy parton is described by fragmentation functions which are introduced through QCD factorizations. While the hadronization mechanism per se remains uknown, fragmentation functions can still be investigated qualitatively and quantitatively. The qualitative study mainly concentrates on extracting genuine features based on the operator definition in quantum field theory. The quantitative research focuses on describing a variety of experimental data employing the fragmentation function given by the parameterizations or model calculations. With the foundation of the transverse-momentum-dependent factorization, the QCD evolution of leading twist transverse-momentum-dependent fragmentation functions has also been established. In addition, the universality of fragmentation functions has been proven, albeit model-dependently, so that it is possible to perform a global analysis of experimental data in different high-energy reactions. The collective efforts may eventually reveal important information hidden in the shadow of nonperturbative physics. This review covers the following topics: transverse-momentum-dependent factorization and the corresponding QCD evolution, spin-dependent fragmentation functions at leading and higher twists, several experimental measurements and corresponding phenomenological studies, and some model calculations.
The Consumption of Raw Goat Milk Resulted in TBE in Patients in Poland, 2022 “Case Report”
Authors: Wójcik-Fatla Angelina , Krzowska-Firych Joanna , Czajka Krzysztof , Nozdryn-Płotnicka Joanna , Sroka Jacek
Published: April 2023

The alimentary route is the second most important route of tick-borne encephalitis infection. In Poland, the last TBE case due to the consumption of unpasteurized milk or dairy products of infected animals was recorded in 2017 as the fourth documented outbreak of TBEV infection in the country. In this study, two patients infected with TBEV through consumption of unpasteurized goat’s milk from one source are described from a cluster of eight cases. In August and September 2022, a 63- and 67-year-old woman were hospitalized at the Infectious Diseases Clinic of the Institute of Rural Health (Lublin, Poland). The patients denied been recently bitten by a tick, and neither had been vaccinated against TBEV. The disease had a biphasic course. In the first case, the patient suffered from a fever, spine pain, and muscle weakness and paresis of the lower left limb. The second patient suffered from fever, vertigo, headaches, abdominal pain, and diarrhoea. The results of IgM and IgG antibodies were positive in both cases. After three weeks hospitalization, the patients were discharged in good condition. In one case, slight hearing impairment was observed. Vaccination and avoiding the consumption of unpasteurized milk remain the most effective ways to prevent tick-borne encephalitis.
Screening of Domestic Cats from North-Eastern Hungary for Hepatozoon felis and Cytauxzoon europaeus That Cause Infections in Local Wildcat Populations
Authors: Tuska-Szalay Barbara , Boldogh Sándor A. , Farkas Róbert , Rompos Luca , Takács Nóra , Beresnyák Viktor , Izsó Ádám , Kontschán Jenő , Lanszki József , Hornok Sándor
Published: April 2023

Among vector-borne protozoa Hepatozoon felis and Cytauxzoon europaeus are considered emerging species in felids in Europe. To investigate the presence of these two protozoa 127 domestic cats and 4 wildcats were screened by PCRs targeting the 18S rRNA gene of Hepatozoon spp. and piroplasms, as well as the cytb gene of Cytauxzoon spp. The samples were collected inside and outside a region of Hungary, where both protozoan groups are endemic in wildcats. Among domestic cats, one proved to be infected with H. felis. Furthermore, spleen samples of four wildcats were also examined, among which three tested positive for H. felis, and one had co-infection with C. europaeus. Importantly, H. felis from the co-infected wildcat belonged to genogroup II, similarly to H. felis from the positive domestic cat. Based on phylogenetic evidence, this genogroup probably represents a separate species from genogroup I of H. felis, which was hitherto reported from Mediterranean countries in Europe. The two other wildcats also harbored H. felis from genogroup I. Neither Hepatozoon nor Cytauxzoon infections were detected outside the recently discovered endemic region. In conclusion, this study demonstrates for the first time in Europe that H. felis from genogroup II may emerge in free-roaming domestic cats in regions where this protozoan parasite is endemic in wildcats.