bluuebunny/crossref_metadata_2025 huggingface dataset:
Prognostic nomograms integrating preoperative serum lipid derivative and systemic inflammatory marker of patients with non-metastatic colorectal cancer undergoing curative resection
Authors: Huang Dimei , Zheng Shaochu , Huang Fang , Chen Jingyu , Zhang Yuexiang , Chen Yusha , Li Bixun
Published: March 2023

Background

Lipid metabolism and cancer-related inflammation are closely related to the progression and prognosis of colorectal cancer (CRC). Therefore, this study aims to establish novel nomograms based on the combined detection of preoperative blood lipids and systemic inflammatory indicators to predict the overall survival (OS) and cancer-specific survival (CCS) of CRC patients.

Methods

A total of 523 patients with stage I-III CRC in our institute were collected from 2014 to 2018. The independent predictors for OS and CCS were determined by forward stepwise Cox regression for the establishment of prognostic models. The superiorities of different models were compared by concordance index (C-index), Akaike information criterion (AIC) and integrated discrimination improvement analysis. The performance of the nomograms based on the optimal models was measured by the plotting time-dependent receiver operating characteristic curves, calibration curves, and decision curves, and compared with the tumor-node-metastasis (TNM) staging system. The cohort was categorized into low-risk, medium-risk and high-risk groups according to the risk points of the nomogram, and analyzed using Kaplan–Meier curves and log-rank test.

Results

Preoperative TG/HDL-C ratio (THR) ≥ 1.93 and prognostic nutritional index (PNI) ≥ 42.55 were independently associated with favorable outcomes in CRC patients. Six (pT stage, pN stage, histological subtype, perineural invasion, THR and PNI) and seven (pT stage, pN stage, histological subtype, perineural invasion, gross appearance, THR and PNI) variables were chosen to develop the optimal models and construct nomograms for the prediction of OS and CCS. The models had lower AIC and larger C-indexes than other models lacking either or both of THR and PNI, and improved those integrated discrimination ability significantly. The nomograms showed better discrimination ability, calibration ability and clinical effectiveness than TNM system in predicting OS and CCS, and these results were reproducible in the validation cohort. The three risk stratifications based on the nomograms presented significant discrepancies in prognosis.

Conclusion

Preoperative THR and PNI have distinct prognostic value in stage I-III CRC patients. The nomograms incorporated the two indexes provide an intuitive and reliable approach for predicting the prognosis and optimizing individualized therapy of non-metastatic CRC patients, which may be a complement to the TNM staging system.
Effect of HBsAg expression in liver tissue on prognosis of hepatocellular carcinoma after minimally invasive interventional therapy
Authors: Liu Biyu , Wang Qi , Mei Tingting , Zheng Jiasheng , Gao Wenfeng , Yuan Chunwang , Li Kang , Zhang Yonghong
Published: March 2023

Background

The aim of this study was to investigate the association between pathologic markers and prognosis in patients with hepatocellular carcinoma who received transcatheter chemoembolization combined with locoregional ablation therapy.

Methods

This retrospective study included 111 hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC). All patients underwent transcatheter arterial chemoembolization (TACE) combined with locoregional ablation therapy, and received core needle biopsy before therapy in Beijing You ‘an Hospital affiliated to Capital Medical University from January 1, 2013 to December 31, 2016. Demographic, pathological indicators and clinical laboratory data were collected. The cumulative recurrence-free survival (RFS) and overall survival (OS) were calculated and compared by Kaplan-Meier method and Log-rank test, and Cox proportional risk model was used to screen for independent predictors of recurrence and long-term prognosis in HCC patients.

Results

There was a correlation between HBsAg expression in liver tissue and prognosis of HCC patients. Patients with negative HBsAg expression had longer 1-,3- and 5-year RFS rates than positive HBsAg expression (78.3%, 43.5%, 30.4% and 58.5%, 24.5%, 17.0%, P=0.018). Meanwhile,the postoperative 1-,3-and 5-year OS rates of HCC patients in the negative HBsAg expression group were significantly higher than those of HCC patients in the positive HBsAg expression group (100%, 89.1%, 80.4% and 100%, 75.5%, 58.5%, P=0.008).

Conclusions

The prognosis of patients with hepatocellular carcinoma with negative HBsAg expression was better than that with positive HBsAg expression. Accordingly, the expression of the liver HBsAg before combined therapy was a prognostic indicator for OS and RFS. For patients with liver HBsAg positive, follow-up should be strengthened and corresponding intervention measures should be taken to improve prognosis.
Preoperative application of carbon nanoparticles in transoral endoscopic thyroidectomy vestibular approach for papillary thyroid cancer
Authors: Wang Yonghui , Zhang Li , Huang Jinning , Wang Liquan
Published: March 2023

Background

Carbon nanoparticles (CNs) have been widely used in the protection of the parathyroid gland and act as a tracer agent in central lymph node dissection. However, the right time for CN injection has not been well illustrated in the transoral endoscopic thyroidectomy vestibular approach (TOETVA). The purpose of this study was to evaluate the safety and feasibility of the preoperative injection of CNs in TOETVA for papillary thyroid cancer.

Methods

From October 2021 to October 2022, a total of 53 consecutive patients with PTC were retrospectively analyzed. All patients underwent unilateral thyroidectomy via the TOETVA. The patients were divided into the preoperative group (n = 28) and the intraoperative group (n = 25) according to CN injection time. In the preoperative group, 0.2 ml of CNs were injected into the thyroid lobules with malignant nodules 1 h before surgery. The numbers of total central lymph node (CLN) and metastatic central lymph node (CLNM), parathyroid autotransplantation, accidental removal of the parathyroid, and the parathyroid hormone level were recorded and analyzed.

Results

The leakage of CNs happened more frequently in the intraoperative group than in the preoperative group (P = 0.002). The mean number of retrieved CLN and CLNM was similar in the preoperative group and the intraoperative group. In parathyroid protection, more parathyroid was discovered in the preoperative group than in the intraoperative group (1.57 ± 0.54 vs. 1.47 ± 0.50, P = 0.002), but less parathyroid autotransplantation (P = 0.004) and accidental removal of the parathyroid (P = 0.036) were discovered in the preoperative group. However, the PTH level between the two groups was similar after the first day and the first month.

Conclusion

The preoperative injection of CNs is a safe and effective method to protect the parathyroid glands (PGs) in patients with PTC undergoing TOETVA. However, the value of preoperative injection of CNs in TOETVA for central lymph node dissection needs to be further studied.
Case report: Novel homozygous HPGD variant leads to primary hypertrophic osteoarthropathy with intussusception and acro-osteolysis in a Chinese family
Authors: Liu Yi , Wu Yinghui , Tang Hongxia , Liu Fan , Wu Yali , Wang Shasha , Ding Yan , Yin Wei
Published: March 2023

Objective

To perform molecular genetic analysis of a patient diagnosed with primary hypertrophic osteoarthropathy (PHO) with malnourishment, intussusception, and acro-osteolysis.

Case presentation

At the age of 7 years, a boy born to a consanguineous couple was diagnosed with PHO attributed to delayed closure of the cranial suture, eczema, clubbing of fingers, and swelling of the knee and ankle. Clinical characteristics and follow-up data for 3 years were collected and analyzed. Trio whole-exome sequencing (WES) and copy number variant sequencing were used to screen for causative genetic variants. Candidate variants of the patient and his parents were confirmed by Sanger sequencing. When he was 7 years old, trio WES found that he had biallelic novel variants c.498 + 1G > A, inherited from his parents, in the HPGD gene. The patient was markedly malnourished. Ultrasonography and computed tomography showed intussusception with a gradual expansion of the duodenum, localized intestinal wall thickening, and acro-osteolysis. Cross-sectional blood tests showed that the patient had continuously decreased levels of serum 25-hydroxy vitamin D and serum ferritin at the age of 7and 10 years.

Conclusion

PHO due to HPGD defects is rare in pediatric patients, and finding homozygous novel c.498 + 1G > A has expanded the spectrum of causative variants of HPGD and provided a clue for genotype-phenotype correlation analysis. Similar to mouse model results, human HPGD deficiency may also cause abnormal digestive tract development, and related secondary vitamin D deficiency and acro-osteolysis should be considered in HPGD-related PHO.
Electroclinical characteristics of photosensitive epilepsy: A retrospective study of 31 Chinese children and literature review
Authors: Zhang Bo , Chen Tianyu , Hao Xiaosheng , Xin Meiying , Liang Jianmin
Published: March 2023

Objective

The objective of this study was to better understand the clinical features of photosensitive epilepsy (PSE) in Chinese children.

Methods

Thirty-one children with PSE were screened out of 398 children with epilepsy who were consecutively diagnosed by the video-electroencephalogram (VEEG) monitoring method and by using an intermittent photic stimulation (IPS) test. Their EEGs and clinical features were retrospectively analyzed, and their treatment outcomes were followed up.

Results

PSE accounted for 7.79% (31/398) of children with epilepsy during the observation period in our single epilepsy center. The male to female ratio of PSE was 1:3.43, and the average seizure onset age was 7.8 ± 3.28 years. The highest range of frequency sensitivity of the IPS test for the induction of EEG epileptic discharge or electroclinical seizures was within 10–20 Hz. Electroclinical seizures were induced in 41.94% (13/31) of PSE patients by using the IPS test, while EEG discharge without clinical seizures was induced in 58.06% (18/31) of PSE patients. Among all PSE patients, an IPS-positive reaction in the eye-closure state was induced in 83.87% of patients, and this rate was significantly higher than that in the eye-opened state (41.94%) or eye-closed state (35.48%). (Eye-closure IPS stimulation means: make the subjects close their eyes at the beginning of each stimulation, open their eyes at the end of the stimulation, and close their eyes again at the beginning of the next stimulation, and so on. While Eye-closed IPS stimulation means the stimulation is started after 5 s of eye closure, and the subjects are kept closed throughout the whole process.) The common and effective drugs used for single or combined therapy in PSE children were valproic acid and levetiracetam.

Conclusion

This study provides some useful information about electroclinical characteristics in a cohort of 31 PSE children. It may be beneficial for pediatric neurologists in terms of paying more attention to PSE and correctly dealing with it.
Microglia: A pharmacological target for the treatment of age-related cognitive decline and Alzheimer’s disease
Authors: McKee Chloe G. , Hoffos Madison , Vecchiarelli Haley A. , Tremblay Marie-Ève
Published: March 2023

As individuals age, microglia, the resident immune cells of the central nervous system (CNS), become less effective at preserving brain circuits. Increases in microglial inflammatory activity are thought to contribute to age-related declines in cognitive functions and to transitions toward mild cognitive impairment (MCI) and Alzheimer’s disease (AD). As microglia possess receptors for communicating with the CNS environment, pharmacological therapies targeting these pathways hold potential for promoting homeostatic microglial functions within the aging CNS. Preclinical and early phase clinical trials investigating the therapeutic effects of pharmacological agents acting on microglia, including reactive oxygen species, TREM2, fractalkine signaling, the complement cascade, and the NLRP3 inflammasome, are currently underway; however, important questions remain unanswered. Current challenges include target selectivity, as many of the signaling pathways are expressed in other cell types. Furthermore, microglia are a heterogenous cell population with transcriptomic, proteomic, and microscopy studies revealing distinct microglial states, whose activities and abundance shift across the lifespan. For example, homeostatic microglia can transform into pathological states characterized by markers of oxidative stress. Selective pharmacological targeting aimed at limiting transitions to pathological states or promoting homeostatic or protective states, could help to avoid potentially harmful off-target effects on beneficial states or other cell types. In this mini-review we cover current microglial pathways of interest for the prevention and treatment of age-related cognitive decline and CNS disorders of aging focusing on MCI and AD. We also discuss the heterogeneity of microglia described in these conditions and how pharmacological agents could target specific microglial states.
Evaluating the efficacy of inhaled amikacin as an adjunct to intravenous combination therapy (ceftazidime and amikacin) in pediatric cystic fibrosis pulmonary exacerbation
Authors: Rakhshan Amin , Farahbakhsh Nazanin , Khanbabaee Ghamartaj , Tabatabaii Seyed Ahmad , Sadr Saeed , Hassanzad Maryam , Sistanizad Mohammad , Dastan Farzaneh , Hajipour Mahmoud , Bahadori Amir Reza , Mirrahimi Bahador
Published: March 2023

Background:Pseudomonas aeruginosa is the most common microorganism found in the sputum culture of Cystic fibrosis (CF) patients causing the pulmonary destruction. Aminoglycosides have a low diffuse rate from lipid membranes, and respiratory system secretions. Regarding the burden of pulmonary exacerbation caused by the pseudomonas aeruginosa in cystic fibrosis patients in the long term and the limited number of clinical trials focused on appropriate treatment strategies, the present study evaluated the concurrent inhaled and intravenous aminoglycoside antibiotics for pulmonary exacerbation caused by the pseudomonas aeruginosa as a safe and effective treatment in children.

Method: This study was a blinded, randomized clinical trial phase conducted in a tertiary referral pediatric teaching hospital from May 2021 to May 2022. The patients were randomly allocated to receive intravenously administered ceftazidime and Amikacin alone or with inhaled Amikacin. Forced expiratory volume (FEV1), Amikacin via the level, kidney function tests, audiometry, inflammatory markers (erythrocyte sedimentation rate and C-reactive protein), hospital stay, and bacterial eradication rate were compared in two therapy groups.

Results: the average FEV1 has increased by 47% in Neb + group compared to Neb− group following treatment. Hospital stay was lower in Neb + group. No renal toxicity or ototoxicity was observed in both therapy groups. Pseudomonas aeruginosa eradication rate Neb− and Neb + groups were 44% and 69%, respectively (p-value = 0.15).

Conclusion: Concurrent inhaled and intravenous Amikacin is safe and effective to treat Pseudomonas aeruginosa exacerbation in CF patients. Moreover, co-delivery antibiotics’ route treatment increased the eradication rate. Although not statistically significant, never the less, it is clinically relevant. The intervention reduced the length of hospitalization in this group.

Clinical Trial Registration:clinicaltrials.gov, identifier [IRCT20120415009475N10].
Progress on role of ion channels of cardiac fibroblasts in fibrosis
Authors: Xing Chenxv , Bao Limeng , Li Weidong , Fan Hongkun
Published: March 2023

Cardiac fibrosis is defined as excessive deposition of extracellular matrix (ECM) in pathological conditions. Cardiac fibroblasts (CFs) activated by injury or inflammation differentiate into myofibroblasts (MFs) with secretory and contractile functions. In the fibrotic heart, MFs produce ECM which is composed mainly of collagen and is initially involved in maintaining tissue integrity. However, persistent fibrosis disrupts the coordination of excitatory contractile coupling, leading to systolic and diastolic dysfunction, and ultimately heart failure. Numerous studies have demonstrated that both voltage- and non-voltage-gated ion channels alter intracellular ion levels and cellular activity, contributing to myofibroblast proliferation, contraction, and secretory function. However, an effective treatment strategy for myocardial fibrosis has not been established. Therefore, this review describes the progress made in research related to transient receptor potential (TRP) channels, Piezo1, Ca2+release-activated Ca2+(CRAC) channels, voltage-gated Ca2+channels (VGCCs), sodium channels, and potassium channels in myocardial fibroblasts with the aim of providing new ideas for treating myocardial fibrosis.
Cryptococcus neoformans Mar1 function links mitochondrial metabolism, oxidative stress, and antifungal tolerance
Authors: Telzrow Calla L. , Esher Righi Shannon , Cathey Jackson M. , Granek Joshua A. , Alspaugh J. Andrew
Published: March 2023

Introduction: Microbial pathogens undergo significant physiological changes during interactions with the infected host, including alterations in metabolism and cell architecture. The Cryptococcus neoformans Mar1 protein is required for the proper ordering of the fungal cell wall in response to host-relevant stresses. However, the precise mechanism by which this Cryptococcus-specific protein regulates cell wall homeostasis was not defined.

Methods: Here, we use comparative transcriptomics, protein localization, and phenotypic analysis of a mar1D loss-of-function mutant strain to further define the role of C. neoformans Mar1 in stress response and antifungal resistance.

Results: We demonstrate that C. neoformans Mar1 is highly enriched in mitochondria. Furthermore, a mar1Δ mutant strain is impaired in growth in the presence of select electron transport chain inhibitors, has altered ATP homeostasis, and promotes proper mitochondrial morphogenesis. Pharmacological inhibition of complex IV of the electron transport chain in wild-type cells promotes similar cell wall changes as the mar1Δ mutant strain, supporting prior associations between mitochondrial function and cell wall homeostasis. Although Mar1 is not required for general susceptibility to the azole antifungals, the mar1Δ mutant strain displays increased tolerance to fluconazole that correlates with repressed mitochondrial metabolic activity.

Discussion: Together, these studies support an emerging model in which the metabolic activity of microbial cells directs cell physiological changes to allow persistence in the face of antimicrobial and host stress.
RETRACTED: Combined effect of Zinc lysine and biochar on growth and physiology of wheat (Triticum aestivum L.) to alleviate salinity stress
Authors: ul Aibdin Zain , Nafees Muhammad , Rizwan Muhammad , Ahmad Shoaib , Ali Shafaqat , Obaid Wael A. , Alsubeie Moodi Saham , Darwish Doaa Bahaa Eldin , Abeed Amany H. A.
Published: March 2023

Globally, Food security main threaten by abiotic stress like salinity and levels amongst the majority serious environmental stressors which reduce crop yield mass production. Biochar application has received much attention in agricultural practices as it enhances crop quality and production. The present study was carried out to analyze the role of lysine zinc and biochar on growth enhancement of wheat (Triticum aestivum L. cv. PU-2011) under saline stress (EC 7.17 dSm-1). Seeds were sown in pots containing saline soil with and without 2% biochar, and foliar application of Zn-lysine (0, 1.0, and 2.0 mM) was made at different time intervals during plant growth. A combined application of biochar and Zn-lysine 2.0 mM highly improved the physiological attributes such as chlorophyll a (37%), chlorophyll b (60%), total chlorophyll (37%), carotenoids (16%), photosynthesis rate (Pn) 45%, stomatal conductance (gs) 53%, transpiration rate (Tr) 56%, and water use efficiency (WUE) 55%. The levels of malondialdehyde (MDA) 38%, hydrogen peroxide (H2O2) 62%, and electrolyte leakage (EL) 48% were decreased with the combined application of biochar and Zn-lysine 2.0 mM as compared with other treatments. The activities of catalase (CAT) 67%, superoxide dismutase (SOD) 70%, and ascorbate peroxidase (APX) 61% as well as catalase (CAT) 67% were regulated with the combined biochar and Zn-lysine 2.0 mM treatment. Similarly, the combined application of biochar and zinc-lysine (2.0 mM) enhanced the growth and yield attributes such as shoot length (79%), root fresh weight (62%), shoot fresh weight (36%), root dry weight (86%), shoot dry weight (39%), grain weight (57%), and spike length (43%) as compared with untreated control. The concentrations of sodium (Na) decreased whereas potassium (K), iron (Fe), and zinc (Zn) concentrations were enhanced in plants with the combined application of Zn-lysine and biochar. Overall, results showed that the combined application of Zn-lysine (2.0 mM) and biochar significantly inhibited the negative effect of salinity and improved the growth and physiological performance of wheat plants. The combined use of Zn-lysine and biochar might be a practical solution to tackle salt stress in plants, but field studies by growing various crops under varied environmental conditions are needed before any recommendation to farmers.
Sterile line Dexiang074A enhances drought tolerance in hybrid rice
Authors: Li Gengmi , Zhang Tao , Yang Li , Qin Jian , Yang Qianhua , Cao Yingjiang , Luo Jing , Li Xiangzhao , Gao Lei , Chen Qian , He Xingping , Huang Yong , Liu Chuantao , He Ling , Zheng Jiakui , Jiang Kaifeng
Published: March 2023

Heterosis has been widely used in rice breeding, especially in improving rice yield. But it has rarely been studied in rice abiotic stress, including the drought tolerance, which is becoming one of the most important threaten in decreasing rice yield. Therefore, it is essential to studying the mechanism underlying heterosis in improving drought tolerance of rice breeding. In this study, Dexiang074B (074B) and Dexiang074A (074A) served as maintainer lines and sterile lines. Mianhui146 (R146), Chenghui727 (R727), LuhuiH103 (RH103), Dehui8258 (R8258), Huazhen (HZ), Dehui938 (R938), Dehui4923 (R4923), and R1391 served as restorer lines. The progeny were Dexiangyou (D146), Deyou4727 (D4727), Dexiang 4103 (D4103), Deyou8258 (D8258), Deyou Huazhen (DH), Deyou 4938 (D4938), Deyou 4923 (D4923), and Deyou 1391 (D1391). The restorer line and hybrid offspring were subjected to drought stress at the flowering stage. The results showed that Fv/Fm values were abnormal and oxidoreductase activity and MDA content were increased. However, the performance of hybrid progeny was significantly better than their respective restorer lines. Although the yield of hybrid progeny and restorer lines decreased simultaneously, the yield in hybrid offspring is significantly lower than the respective restorer line. Total soluble sugar content was consistent with the yield result, so we found that 074A can enhance drought tolerance in hybrid rice.
GIGANTEA supresses wilt disease resistance by down-regulating the jasmonate signaling in Arabidopsis thaliana
Authors: Patnaik Alena , Kumar Aman , Behera Anshuman , Mishra Gayatri , Dehery Subrat Kumar , Panigrahy Madhusmita , Das Anath Bandhu , Panigrahi Kishore C. S.
Published: March 2023

GIGANTEA (GI) is a plant-specific nuclear protein that plays a pleiotropic role in the growth and development of plants. GI’s involvement in circadian clock function, flowering time regulation, and various types of abiotic stress tolerance has been well documented in recent years. Here, the role of GI in response to Fusarium oxysporum (F. oxysporum) infection is investigated at the molecular level comparing Col-0 WT with the gi-100 mutant in Arabidopsis thaliana. Disease progression, photosynthetic parameters, and comparative anatomy confirmed that the spread and damage caused by pathogen infection were less severe in gi-100 than in Col-0 WT plants. F. oxysporum infection induces a remarkable accumulation of GI protein. Our report showed that it is not involved in flowering time regulation during F. oxysporum infection. Estimation of defense hormone after infection showed that jasmonic acid (JA) level is higher and salicylic acid (SA) level is lower in gi-100 compared to Col-0 WT. Here, we show that the relative transcript expression of CORONATINE INSENSITIVE1 (COI1) and PLANT DEFENSIN1.2 (PDF1.2) as a marker of the JA pathway is significantly higher while ISOCHORISMATE SYNTHASE1 (ICS1) and NON-EXPRESSOR OF PATHOGENESIS-RELATED GENES1 (NPR1), the markers of the SA pathway, are downregulated in the gi-100 mutants compared to Col-0 plants. The present study convincingly suggests that the GI module promotes susceptibility to F. oxysporum infection by inducing the SA pathway and inhibiting JA signaling in A. thaliana.
LMTdb: A comprehensive transcriptome database for climate-resilient, nutritionally rich little millet (Panicum sumatrense)
Authors: Shekhar Shweta , Prasad Archana S. , Banjare Kalpana , Kaushik Abhijeet , Mannade Ajit K. , Dubey Mahima , Patil Arun , Premi Vinay , Vishwakarma Ashish K. , Sao Abhinav , Saxena Ravi R. , Dubey Amit , Chandel Girish
Published: March 2023

Little millet (Panicum sumatrense) a native of Chhattisgarh, belongs to the minor millet group and is primarily known as a climate-resilient and nutritionally rich crop. However, due to the lack of enough Omic studies on the crop, the scientific community has largely remained unaware of the potential of this crop, resulting in less scope for its utilization in crop improvement programs. Looking at global warming, erratic climate change, nutritional security, and limited genetic information available, the Little Millet Transcriptome Database (LMTdb) (https://igkv.ac.in/xenom/index.aspx) was conceptualized upon completion of the transcriptome sequencing of little millet with the aim of deciphering the genetic signatures of this largely unknown crop. The database was developed with the view of providing information about the most comprehensive part of the genome, the ‘Transcriptome’. The database includes transcriptome sequence information, functional annotation, microsatellite markers, DEGs, and pathway information. The database is a freely available resource that provides breeders and scientists a portal to search, browse, and query data to facilitate functional and applied Omic studies in millet crops.
Hormones regulate the flowering process in saffron differently depending on the developmental stage
Authors: Singh Deepika , Sharma Sahiba , Jose-Santhi Joel , Kalia Diksha , Singh Rajesh Kumar
Published: March 2023

Flowering in saffron is a highly complex process regulated by the synchronized action of environmental cues and endogenous signals. Hormonal regulation of flowering is a very important process controlling flowering in several plants, but it has not been studied in saffron. Flowering in saffron is a continual process completed in months with distinct developmental phases, mainly divided into flowering induction and flower organogenesis/formation. In the present study, we investigated how phytohormones affect the flowering process at different developmental stages. The results suggest that different hormones differentially affect flower induction and formation in saffron. The exogenous treatment of flowering competent corms with abscisic acid (ABA) suppressed both floral induction and flower formation, whereas some other hormones, like auxins (indole acetic acid, IAA) and gibberellic acid (GA), behaved contrarily at different developmental stages. IAA promoted flower induction, while GA suppressed it; however, GA promoted flower formation, whereas IAA suppressed it. Cytokinin (kinetin) treatment suggested its positive involvement in flower induction and flower formation. The expression analysis of floral integrator and homeotic genes suggests that ABA might suppress floral induction by suppressing the expression of the floral promoter (LFY, FT3) and promoting the expression of the floral repressor (SVP) gene. Additionally, ABA treatment also suppressed the expression of the floral homeotic genes responsible for flower formation. GA reduces the expression of flowering induction gene LFY, while IAA treatment upregulated its expression. In addition to these genes, a flowering repressor gene, TFL1-2, was also found to be downregulated in IAA treatment. Cytokinin promotes flowering induction by increasing the expression levels of the LFY gene and decreasing the TFL1-2 gene expression. Moreover, it improved flower organogenesis by increasing the expression of floral homeotic genes. Overall, the results suggest that hormones differently regulate flowering in saffron via regulating floral integrator and homeotic gene expression.
Comparative metabolomic profiling of Arabidopsis thaliana roots and leaves reveals complex response mechanisms induced by a seaweed extract
Authors: Tran Thi Linh Chi , Callahan Damien L. , Islam Md Tohidul , Wang Yichao , Arioli Tony , Cahill David
Published: March 2023

Seaweed extracts are a prominent class of biostimulants that enhance plant health and tolerance to biotic and abiotic stresses due to their unique bioactive components. However, the mechanisms of action of biostimulants are still unknown. Here, we have used a metabolomic approach, a UHPLC-MS method, to uncover the mechanisms induced following application to Arabidopsis thaliana of a seaweed extract derived from Durvillaea potatorum and Ascophyllum nodosum. We have identified, following the application of the extract, key metabolites and systemic responses in roots and leaves across 3 timepoints (0, 3, 5 days). Significant alterations in metabolite accumulation or reduction were found for those belonging to broad groups of compounds such as lipids, amino acids, and phytohormones; and secondary metabolites such as phenylpropanoids, glucosinolates, and organic acids. Strong accumulations of TCA cycle and N-containing and defensive metabolites such as glucosinolates were also found revealing the enhancement of carbon and nitrogen metabolism and defence systems. Our study has demonstrated that application of seaweed extract dramatically altered the metabolomic profiles of Arabidopsis and revealed differences in roots and leaves that varied across the timepoints tested. We also show clear evidence of systemic responses that were initiated in the roots and resulted in metabolic alterations in the leaves. Collectively, our results suggest that this seaweed extract promotes plant growth and activates defence systems by altering various physiological processes at the individual metabolite level.
Multi-walled carbon nanotubes promote the accumulation, distribution, and assimilation of 15N-KNO3 in Malus hupehensis by entering the roots
Authors: Shi Junyuan , Xun Mi , Song Jianfei , Li Jiaqi , Zhang Weiwei , Yang Hongqiang
Published: March 2023

Introduction

Multi-walled nanotubes (MWCNTs) consist of multiple rolled layers of graphene. Nitrogen plays an important role in apple growth. The effect of MWCNTs on nitrogen utilization in apple needs to be further investigated.

Methods

In this study, the woody plant Malus hupehensis seedlings were used as plant materials, the distribution of MWCNTs in the roots was observed, and the effects of MWCNTs on the accumulation, distribution, and assimilation of nitrate by the seedlings were explored.

Results

The results showed that MWCNTs could penetrate the roots of Malus hupehensis seedlings, and the 50, 100, and 200 µg·mL-1 MWCNTs significantly promoted the root growth of seedlings, increased root number, root activity, fresh weight, and nitrate content of seedlings, and also increased nitrate reductase activity, free amino acid, and soluble protein content of roots and leaves. 15N tracer experiments indicated that MWCNTs decreased the distribution ratio of 15N-KNO3 in Malus hupehensis roots but increased its distribution ratio in stems and leaves. MWCNTs improved the utilization ratio of 15N-KNO3 in Malus hupehensis seedlings, with the values being increased by 16.19%, 53.04%, and 86.44% following the 50, 100, and 200 µg·mL-1 MWCNTs, respectively. The RT-qPCR analysis showed that MWCNTs significantly affected the expression of genes (MhNRTs) related to nitrate uptake and transport in roots and leaves, and MhNRT1.4, MhNRT1.7, MhNRT1.8, MhNRT2.1, MhNRT2.5, and MhNRT2.7 were notably up-regulated in response to 200 µg·mL-1 MWCNTs. Raman analysis and transmission electron microscopy images indicated that MWCNTs could enter the root tissue of Malus hupehensis and were distributed between the cell wall and cytoplasmic membrane. Pearson correlation analysis showed that root tip number, root fractal dimension, and root activity were the main factors affecting root uptake and assimilation of nitrate.

Conclusions

These findings suggest that MWCNTs promoted root growth by entering the root, stimulated the expression of MhNRTs, and increased NR activity, thereby enhancing the uptake, distribution, and assimilation of nitrate by root, and ultimately improved the utilization of 15N-KNO3 by Malus hupehensis seedlings.
Sensor feedback system enables automated deficit irrigation scheduling for cotton
Authors: O’Shaughnessy Susan A. , Colaizzi Paul D. , Bednarz Craig W.
Published: March 2023

Precision irrigation technologies using sensor feedback can provide dynamic decision support to help farmers implement DI strategies. However, few studies have reported on the use of these systems for DI management. This two-year study was conducted in Bushland, Texas to investigate the performance of the geographic information (GIS) based irrigation scheduling supervisory control and data acquisition (ISSCADA) system as a tool to manage deficit irrigation scheduling for cotton (Gossypim hirsutumL). Two different irrigation scheduling methods automated by the ISSCADA system — (1) a plant feedback (designated C) - based on integrated crop water stress index (iCWSI) thresholds, and (2) a hybrid (designated H) method, created to combine soil water depletion and theiCWSI thresholds, were compared with a benchmark manual irrigation scheduling (M) that used weekly neutron probe readings. Each method applied irrigation at levels designed to be equivalent to 25%, 50% and 75% replenishment of soil water depletion to near field capacity (designated I25, I50and I75) using the pre-established thresholds stored in the ISSCADA system or the designated percent replenishment of soil water depletion to field capacity in the M method. Fully irrigated and extremely deficit irrigated plots were also established. Relative to the fully irrigated plots, deficit irrigated plots at the I75level for all irrigation scheduling methods-maintained seed cotton yield, while saving water. In 2021, the irrigation savings was a minimum of 20%, while in 2022, the minimum savings was 16%. Comparing the performance of deficit irrigation scheduling between the ISSCADA system and the manual method showed that crop response for all three methods were statistically similar at each irrigation level. Because the M method requires labor intensive and expensive use of the highly regulated neutron probe, the automated decision support provided by the ISSCADA system could simplify deficit irrigation management of cotton in a semi-arid region.
Variation in the chemical profiles of three foxglove species in the central Balkans
Authors: Gašić Uroš , Banjanac Tijana , Šiler Branislav , Božunović Jelena , Milutinović Milica , Aničić Neda , Dmitrović Slavica , Skorić Marijana , Nestorović Živković Jasmina , Petrović Luka , Todorović Miloš , Živković Suzana , Matekalo Dragana , Filipović Biljana , Lukić Tamara , Mišić Danijela
Published: March 2023

The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species (D. lanata, D. ferruginea, and D. grandiflora) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis (Plantaginaceae) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora. The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p-coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p-coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea. However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis.
Factors influencing Chinese public attitudes toward farm animal welfare
Authors: Jiang Bing , Tang Wenjie , Cui Lihang , Wei Yanjiao
Published: March 2023

A comprehensive understanding of current Chinese public attitudes toward farm animal welfare and the relevant influencing factors is essential for improving farm animal welfare and promoting further development of animal husbandry. The attitudes of 3,726 respondents in China were investigated using paper and online questionnaires. Three components (affective, cognitive, and behavioral) of attitudes toward farm animal welfare were assessed using 18 items designed based on the literature review. Influential factors of attitudes toward farm animal welfare were explored via tobit regression. The results revealed that the Chinese public not only considers farm animals to be emotional and sentient but are also sympathetic toward farm animals that suffer inhumane treatment. Although they have limited knowledge about farm animal welfare, the public believes improving farm animal welfare is beneficial, especially for food safety and human health. The Chinese public prefers regulation policies to incentive policies for improving farm animal welfare. The main factors influencing attitudes toward farm animal welfare included gender, age, education, monthly household income, area of residence, farm animal raising experience, and attention to farm animal welfare events. The effect of these influencing factors on attitudes varied. These findings provide a basis for improving Chinese public attitudes toward farm animal welfare. The implications of formulating and implementing effective policies to improve the Chinese public attitudes toward farm animal welfare were discussed.
How automation level influences moral decisions of humans collaborating with industrial robots in different scenarios
Authors: Eich Anne , Klichowicz Anja , Bocklisch Franziska
Published: March 2023

Introduction

Digitalization in intelligent manufacturing leads to the development of Industry 4.0/5.0 and human-cyber-physical systems. As many production technologies rely on teaming of human workers and intelligent cyber-physical systems such as industrial robots, human-robot collaboration is an intensively investigated topic in this transdisciplinary research area. To design industrial robots in a human-centered way, psychological knowledge concerning judgment and decision-making needs to be gained and integrated.

Method

This paper reports results from an experimental study (N = 222, 2 × 4 within-subjects design) using eight moral dilemmas framed in the context of human-robot-collaboration to examine the influence of spatial distance of an industrial robot and humans (no direct contact, different tasks vs. no direct contact, same task vs. handing-over contact, same task vs. direct contact, same task) on moral decisions. Additionally, the type of dilemma was varied, with every four dilemmas depicting a life-or-death and an injury scenario. Participants responded on a four-point-response scale which actions they would take indicating deontological or utilitarian moral decision-making.

Results

Results show a large effect of the proximity of the cooperation between robots and humans. The closer the collaboration the more a human tends to choose utilitarian moral choices.

Discussion

It is argued that this effect might stem from an adaptation of human rationality to the robot or overreliance and shift of responsibility to the robot team partner.
How to mitigate the inhibitory effect of organizational inertia on corporate digital entrepreneurship?
Authors: Li Wei , Chen Wei , Pang Qingdan , Song Jianmin
Published: March 2023

As a novel way for incumbent firms to discover and utilize entrepreneurial opportunities in the digital era, corporate digital entrepreneurship (CDE) is significant for realizing digital transformation through dealing with organizational sclerosis and bureaucratization. Previous studies have identified the variables having positive effects on CDE and put forward practical solutions to promoting CDE. However, the majority of them have ignored the variables having negative effects on CDE and how to mitigate the inhibitory effects. In order to fill the research gap, this study investigates the causal relationship between organizational inertia (OI) and CDE and examines the moderating roles of internal factors such as digital capability (DC) and entrepreneurial culture (EC) as well as external factors such as institutional support (IS) and strategic alliance (SA). Based on multiple linear regression (symmetric) and fuzzy-set qualitative comparative analysis (asymmetric) using survey data from 349 Chinese firms, the results demonstrate that OI has a significant negative effect on CDE. In addition, DC, EC, and SA play negative moderating roles in the relationship between OI and CDE, which means that they could reduce the inhibitory effect derived from OI when incumbent firms implement CDE. Moreover, dividing OI into three dimensions discovers that the moderating roles of DC, EC, and SA present different features. This study enriches the literature on corporate entrepreneurship and provides valuable practical implications for incumbent firms to achieve successful CDE by revealing how to overcome the inertia deeply embedded in organizations.
Improving adherence to immunosuppression after liver or kidney transplantation in individuals with impairments in personality functioning – A randomized controlled single center feasibility study
Authors: Wagner-Skacel Jolana , Fink Nadja , Kahn Judith , Dalkner Nina , Jauk Emanuel , Bengesser Susanne , Mairinger Marco , Schüssler Gerhard , Pieh Christoph , Stadlbauer Vanessa , Kirsch Alexander H. , Zitta Sabine , Rosenkranz Alexander R. , Fickert Peter , Schemmer Peter
Published: March 2023

Introduction

Although adherence to immunosuppressive medication is the key factor for long-term graft survival today, 20–70% of transplant recipients are non-adherent to their immunosuppressive medication.

Objective

A prospective, randomized, controlled single-center feasibility study was designed to evaluate the impact of a step guided multicomponent interprofessional intervention program for patients after kidney or liver transplantation on adherence to their immunosuppressive medication in daily clinical practice.

Materials and methods

The intervention consisted of group therapy and daily training as well as individual sessions in a step guided approach. The primary endpoint of the study was adherence to immunosuppression as assessed with the “Basel Assessment of Adherence to Immunosuppressive Medications Scale” (BAASIS). The coefficient of variation (CV%) of Tacrolimus (TAC) through levels and the level of personality functioning was a secondary endpoint. We conducted six monthly follow-up visits.

Results

Forty-one age- and sex-matched patients [19 females, 58.5 (SD = 10.56) years old, 22 kidney- and 19 liver transplantation] were randomized to the intervention- (N = 21) or control-group (N = 20). No differences between intervention- and control groups were found in the primary endpoint adherence and CV% of TAC. However, in further exploratory analyses, we observed that individuals with higher impairments in personality functioning showed higher CV% of TAC in the controls. The intervention might compensate personality-related susceptibility to poor adherence as evident in CV% of TAC.

Discussion

The results of the feasibility study showed that this intervention program was highly accepted in the clinical setting. The Intervention group could compensate higher CV% of TAC after liver or kidney transplantation in individuals with lower levels of personality functioning and non-adherence.

Clinical trial registration

ClinicalTrials.gov, identifier NCT04207125.
Factors that favor or hinder the acquisition of a digital culture in large organizations in Chile
Authors: Busco Carolina , González Felipe , Aránguiz Michelle
Published: March 2023

Organizational culture is often perceived as a valuable strategic asset supporting business transformation and the exploitation of digital technologies. Still, it can also be the source of inertia that impedes change. The research question proposed is What factors favor or hinder the acquisition of digital culture in large organizations in Chile? The aim is to rank factors that promote a digital culture based on the perception of executives using the Delphi method. The expert panel was selected with strategic criteria, considering practical knowledge, up-to-date experience on the subject, and having high decision-making positions in large companies in Chile. The main statistics used are media, maximum, minimum, and average range, along with the search for consensus determined by the interquartile range and Kendall’s W concordance coefficient. Results show a high level of agreement on the importance of digital strategy and digital leadership factors when favoring a digital culture in large companies in Chile. However, large companies in Chile must pay attention to the conservative triad of elements that characterize Chilean work culture that considers the belief that changes are exclusively possible when commanded by the strategic apex, a hierarchical work culture that prevents collaborative work, and the rejection of disruptive change. These factors and cultural characteristics will likely hinder any attempt to succeed in a digital transformation plan.