bluuebunny/crossref_metadata_2025 huggingface dataset:
Polarization-Based Histopathology Classification of Ex Vivo Colon Samples Supported by Machine Learning
Authors: Ivanov Deyan , Dremin Viktor , Genova Tsanislava , Bykov Alexander , Novikova Tatiana , Ossikovski Razvigor , Meglinski Igor
Published: January 2022

In biophotonics, novel techniques and approaches are being constantly sought to assist medical doctors and to increase both sensitivity and specificity of the existing diagnostic methods. In such context, tissue polarimetry holds promise to become a valuable optical diagnostic technique as it is sensitive to tissue alterations caused by different benign and malignant formations. In our studies, multiple Mueller matrices were recorded for formalin-fixed, human,ex vivocolon specimens containing healthy and tumor zones. The available data were pre-processed to filter noise and experimental errors, and then all Mueller matrices were decomposed to derive polarimetric quantities sensitive to malignant formations in tissues. In addition, the Poincaré sphere representation of the experimental results was implemented. We also used the canonical and natural indices of polarimetric purity depolarization spaces for plotting our experimental data. A feature selection was used to perform a statistical analysis and normalization procedure on the available data, in order to create a polarimetric model for colon cancer assessment with strong predictors. Both unsupervised (principal component analysis) and supervised (logistic regression, random forest, and support vector machines) machine learning algorithms were used to extract particular features from the model and for classification purposes. The results from logistic regression allowed to evaluate the best polarimetric quantities for tumor detection, while the use of random forest yielded the highest accuracy values. Attention was paid to the correlation between the predictors in the model as well as both losses and relative risk of misclassification. Apart from the mathematical interpretation of the polarimetric quantities, the presented polarimetric model was able to support the physical interpretation of the results from previous studies and relate the latter to the samples’ health condition, respectively.
Biomechanical Finite Element Method Model of the Proximal Carpal Row and Experimental Validation
Authors: Marqués Rafael , Melchor Juan , Sánchez-Montesinos Indalecio , Roda Olga , Rus Guillermo , Hernández-Cortés Pedro
Published: January 2022

The Finite Element Method (FEM) models are valuable tools to create an idea of the behavior of any structure. The complexity of the joints, materials, attachment areas, and boundary conditions is an open issue in biomechanics that needs to be addressed. Scapholunate instability is the leading cause of wrist pain and disability among patients of all ages. It is needed a better understanding of pathomechanics to develop new effective treatments. Previous models have emulated joints like the ankle or the knee but there are few about the wrist joint. The elaboration of realistic computational models of the carpus can give critical information to biomedical research and surgery to develop new surgical reconstructions. Hence, a 3D model of the proximal carpal row has been created through DICOM images, making a reduced wrist model. The materials, contacts, and ligaments definition were made via open-source software to extract results and carry on a reference comparison. Thus, considering the limitations that a reduced model could carry on (unbalanced forces and torques), the stresses that result in the scapholunate interosseous ligament (SLIL) lead us to a bones relative displacement, which support the kinematics hypothesis in the literature as the distal carpal row moves as a rigid solid with the capitate bone. Also, experimental testing is performed, successfully validating the linear strength values of the scapholunate ligament from the literature.
Transcriptome Analysis for Identification of Genes Related to Growth and Development, Digestion and Detoxification, Olfaction in the Litchi Stink Bug Tessaratoma papillosa
Authors: Cheng Lin , Han Shuncai , Jiang Jingtao , Li Haichao , Peng Lingfei
Published: January 2022

Tessaratoma papillosa is a major pest of Litchi chinensis and Dimocarpus longan. Adult and nymph secretions are not only harmful to plants but also to humans. At present, there are not a lot of research on T. papillosa, especially omics research. We used high-throughput sequencing technology to sequence the T. papillosa transcriptome and obtained 67,597 unigenes homologous to Halyomorpha halys (88.03%). Subsequently, RNA-SEQ and comparative analyses were performed on the 14 different developmental stages and tissues. A total of 462 unigenes related to growth and development, 1,851 unigenes related to digestion and detoxification, and 70 unigenes related to olfaction were obtained. Moreover, expression analysis showed that the T. papillosa major life activities genes are uniformly expressed across all developmental states. However, the adult midgut gene expression patterns were utterly different from that of the nymphs. Similarly, female fat body genes exhibited distinct expression patterns compared to that of males and nymphs. Thus, different developmental stages and physiological functions affect gene expression patterns. We also found that most of the differential genes were associated with cellular maintenance. This study will help understand the growth and development of litchi stink bugs, their choice of host plants, food digestion and detoxification, and their reproductive behavior. In addition, this result can provide reference information for some target genes in the process of control of T. papillosa.
Local Electrical Impedance Mapping of the Atria: Conclusions on Substrate Properties and Confounding Factors
Authors: Unger Laura Anna , Schicketanz Leonie , Oesterlein Tobias , Stritt Michael , Haas Annika , Martínez Antón Carmen , Schmidt Kerstin , Doessel Olaf , Luik Armin
Published: January 2022

The treatment of atrial fibrillation and other cardiac arrhythmias as a major cause of cardiovascular hospitalization has remained a challenge predominantly for patients with severely remodeled substrate. Individualized ablation strategies are extremely important both for pulmonary vein isolation and subsequent ablations. Current approaches to identifying arrhythmogenic regions rely on electrogram-based features such as activation time and voltage. Novel technologies now enable clinical assessment of the local impedance as tissue property. Previous studies demonstrated its use for ablation monitoring and indicated its potential to differentiate healthy substrate, scar, and pathological tissue. This study investigates the potential of local electrical impedance-based substrate mapping of the atria for human in-vivo data. The presented pipeline for impedance mapping particularly contains options for dealing with undesirable effects originating from cardiac motion, catheter motion, or proximity to other intracardiac devices. Bloodpool impedance was automatically determined as a patient-specific reference. Full-chamber, left atrial impedance maps were drawn up from interpolating the measured impedances to the atrial endocardium. Finally, the origin and magnitude of oscillations of the raw impedance recording were probed into. The most dominant reason for exclusion of impedance samples was the loss of endocardial contact. With median elevations above the bloodpool impedance between 29 and 46 Ω, the impedance within the pulmonary veins significantly exceeded the remaining atrial walls presenting median elevations above the bloodpool impedance between 16 and 20 Ω. Previous ablation lesions were distinguished from their surroundings by a significant drop in local impedance while the corresponding regions did not differ for the control group. The raw impedance was found to oscillate with median amplitudes between 6 and 17 Ω depending on the patient. Oscillations were traced back to an interplay of atrial, ventricular, and respiratory motion. In summary, local impedance measurements demonstrated their capability to distinguish pathological atrial tissue from physiological substrate. Methods to limit the influence of confounding factors that still hinder impedance mapping were presented. Measurements at different frequencies or the combination of multiple electrodes could lead to further improvement. The presented examples indicate that electrogram- and impedance-based substrate mapping have the potential to complement each other toward better patient outcomes in future.
To What Degree Does Limb Spasticity Affect Motor Performance in Para-Footballers With Cerebral Palsy?
Authors: Roldan Alba , Henríquez Matías , Iturricastillo Aitor , Castillo Daniel , Yanci Javier , Reina Raul
Published: January 2022

Spasticity is considered a contributor to hypertonia, frequently presented in people with cerebral palsy (CP), affecting muscle function and motor activities. In CP football, the classification system determines that this impairment is eligible for competitive para-sports due to the impact on activity limitation and sports performance. However, the relationship between this feature (i.e., spastic hypertonia) and performance determinants has not been explored yet. This study aimed to assess the association of clinical spasticity measurements with the performance of sport-specific tests used for classification purposes. Sixty-nine international footballers with CP voluntarily participated in this study. The Australian Spasticity Assessment Scale was used to measure spasticity in lower limbs muscle groups and activity limitation tests were conducted considering dynamic balance, coordination, vertical and horizontal jumps, acceleration, and change of direction ability. Low-to-moderate negative significant associations were found between the hip spasticity and measures of dynamic balance and dominant unipedal horizontal jump capacity. Additionally, moderate associations were reported between the knee spasticity and the non-dominant unipedal horizontal jump capacity and the change of direction actions with the ball. The ankle spasticity score reported small to moderate associations with the change of direction assessment without the ball and bipedal and dominant unipedal horizontal jump capability. Finally, the total spasticity score only presented a significant association with horizontal jump performance. This is a novel study that provides evidence of the associations between an eligible neural impairment and relevant specific measures of activity limitation tests. These results suggest that the amount of spasticity according to each evaluated joint muscle group of the lower limbs presents a low-to-moderate significant relationship with determined measures of dynamic balance, coordination, horizontal jump, acceleration, and change of direction ability with and without the ball in international-level CP footballers. Further studies are necessary to elucidate the real contribution of neural and non-neural impairments related to hypertonia on fundamental sport-specific motor skills of para-footballers with CP.
Visualization of Dynamic Mitochondrial Calcium Fluxes in Isolated Cardiomyocytes
Authors: Krstic Anna Maria , Power Amelia Sally , Ward Marie-Louise
Published: January 2022

Background

Cardiomyocyte contraction requires a constant supply of ATP, which varies depending on work rate. Maintaining ATP supply is particularly important during excitation-contraction coupling, where cytosolic Ca2+ fluxes drive repeated cycles of contraction and relaxation. Ca2+ is one of the key regulators of ATP production, and its uptake into the mitochondrial matrix occurs via the mitochondrial calcium uniporter. Fluorescent indicators are commonly used for detecting cytosolic Ca2+ changes. However, visualizing mitochondrial Ca2+ fluxes using similar methods is more difficult, as the fluorophore must be permeable to both the sarcolemma and the inner mitochondrial membrane. Our aim was therefore to optimize a method using the fluorescent Ca2+ indicator Rhod-2 to visualize beat-to-beat mitochondrial calcium fluxes in rat cardiomyocytes.

Methods

Healthy, adult male Wistar rat hearts were isolated and enzymatically digested to yield rod-shaped, quiescent ventricular cardiomyocytes. The fluorescent Ca2+ indicator Rhod-2 was reduced to di-hydroRhod-2 and confocal microscopy was used to validate mitochondrial compartmentalization. Cardiomyocytes were subjected to various pharmacological interventions, including caffeine and β-adrenergic stimulation. Upon confirmation of mitochondrial Rhod-2 localization, loaded myocytes were then super-fused with 1.5 mM Ca2+ Tyrodes containing 1 μM isoproterenol and 150 μM spermine. Myocytes were externally stimulated at 0.1, 0.5 and 1 Hz and whole cell recordings of both cytosolic ([Ca2+]cyto) and mitochondrial calcium ([Ca2+]mito) transients were made.

Results

Myocytes loaded with di-hydroRhod-2 revealed a distinct mitochondrial pattern when visualized by confocal microscopy. Application of 20 mM caffeine revealed no change in fluorescence, confirming no sarcoplasmic reticulum compartmentalization. Myocytes loaded with di-hydroRhod-2 also showed a large increase in fluorescence within the mitochondria in response to β-adrenergic stimulation (P < 0.05). Beat-to-beat mitochondrial Ca2+ transients were smaller in amplitude and had a slower time to peak and maximum rate of rise relative to cytosolic calcium transients at all stimulation frequencies (P < 0.001).

Conclusion

Myocytes loaded with di-hydroRhod-2 revealed mitochondrial specific compartmentalization. Mitochondrial Ca2+ transients recorded from di-hydroRhod-2 loaded myocytes were distinct in comparison to the large and rapid Rhod-2 cytosolic transients, indicating different kinetics between [Ca2+]cyto and [Ca2+]mito transients. Overall, our results showed that di-hydroRhod-2 loading is a quick and suitable method for measuring beat-to-beat [Ca2+]mito transients in intact myocytes.
Anopheles gambiae Actively Metabolizes Uric Acid Following Plasmodium Infection to Limit Malaria Parasite Survival
Authors: Kwon Hyeogsun , Smith Ryan
Published: January 2022

Characterizing the physiological changes that accompany malaria parasite infection of the mosquito host is crucial to our understanding of vectorial capacity in Anopheles mosquitoes, yet has not fully been explored. In this study, we examine the role of uric acid metabolism in the mosquito, Anopheles gambiae, following malaria parasite infection. We demonstrate that levels of uric acid are significantly decreased in the excreta and the mosquito at 24 and 48 h post-Plasmodium infection when compared to controls fed on naïve mouse blood. When we examine the expression of well-known enzymes responsible for uric acid metabolism, we see a significant increase in both urate oxidase (UO) and allatoicase (ALLC) expression following Plasmodium infection. Targeting the essential first step in uric acid metabolism by silencing UO resulted in elevated levels of uric acid, enhancing malaria parasite survival. With implications from other insect systems that bacteria can modulate UO expression, we examined the possibility that the mosquito microbiota and its expansion following blood-feeding may contribute to increased UO levels. However, there was no difference in uric acid metabolism between septic and aseptic mosquitoes, indicating that the mosquito microbiome is not associated with the manipulation of UO expression. Together, our study provides new evidence that Plasmodium infection causes the mosquito host to actively metabolize uric acid by increasing UO expression to limit Plasmodium oocyst survival, suggesting that nitrogen metabolism is an essential pathway in defining mosquito vector competence.
School-Aged Children Learn Novel Categories on the Basis of Distributional Information
Authors: Broedelet Iris , Boersma Paul , Rispens Judith
Published: January 2022

Categorization of sensory stimuli is a vital process in understanding the world. In this paper we show that distributional learning plays a role in learning novel object categories in school-aged children. An 11-step continuum was constructed based on two novel animate objects by morphing one object into the other in 11 equal steps. Forty-nine children (7–9 years old) were subjected to one of two familiarization conditions during which they saw tokens from the continuum. The conditions differed in the position of the distributional peaks along the continuum. After familiarization it was tested how the children categorized the stimuli. Results show that, in line with our expectations, familiarization condition influenced categorization during the test phase, indicating that the frequency distribution of tokens in the input had induced novel object category formation. These results suggest that distributional learning could play an important role in categorizing sensory stimuli throughout life.
Computer Based Body Exposure in Adolescents With Anorexia Nervosa: A Study Protocol
Authors: Stonawski Valeska , Sasse Lena , Moll Gunther , Kratz Oliver , Horndasch Stefanie
Published: January 2022

Body dissatisfaction is a core feature of eating disorders (EDs) and plays an essential role in the development and maintenance of anorexia nervosa (AN). In the current study, a computer based body exposure intervention is conducted and evaluated regarding short-term effects on body dissatisfaction, psychopathology, viewing patterns, and stress reactivity. Within a randomized controlled trial (RCT) female adolescents and young women with AN are either receiving the intervention or treatment as usual (TAU). Furthermore, in a transdiagnostic approach, a highly body-dissatisfied group of clinical control participants obtaining the intervention will be surveyed to identify AN-specific processes. The standardized four-session body exposure intervention using photographs of the own body is adapted from a manualized body image treatment program for computer use. Psychopathology (body dissatisfaction, body image avoidance, body checking, depression, anxiety) is assessed via standardized questionnaires before and after the intervention. During each session, attentional biases regarding one's own body are measured via eye tracking, stress levels are measured via subjective ratings, heart rate variability, as well as salivary cortisol and alpha amylase. Between- and within-subject effects will be assessed. The pilot study aims to identify short-term effects of the intervention on body dissatisfaction and attentional bias, as well as to investigate the potential underlying mechanism of physiological habituation.
Epidemiological Characteristics and Their Influencing Factors Among Pulmonary Tuberculosis Patients With and Without Diabetes Mellitus: A Survey Study From Drug Resistance Surveillance in East China
Authors: Wu Qian , Wang Min , Zhang Yu , Wang Wei , Ye Teng-Fei , Liu Kui , Chen Song-Hua
Published: January 2022

Background

The burden of pulmonary tuberculosis (TB) and diabetes mellitus (DM) have become serious global concerns, while the comprehensive evaluations of DM status and drug resistance in TB patients are still lacking.

Methods

All details of TB cases were collected from drug resistance monitoring sentinels in Zhejiang province. Fisher's exact test or Pearson chi-square test (χ2) was used to compare the baseline characteristics among TB with different DM statuses. The logistic regression model was used to estimate the relationship between DM and different drug resistance spectra. Univariate analysis and multivariate logistic model were used to explore the possible risk factors of drug resistance in TB patients with DM and no DM.

Results

936 TB cases with smear-positive in Zhejiang province were collected, in which 76 patients (8.12%) owned the co-morbidity of DM. TB-DM prevalence was higher in older, Han nationality, employed, accompanied by no health insurance and hepatitis B status. Among 860 cases of TB-no DM and 76 cases of TB-DM, drug resistance-TB accounted for 31.51% and 23.68% (P> 0.05), MR-TB accounted for 15.93% and 14.47% (P> 0.05), respectively. MDR-TB was 4.88% and 6.58% (P> 0.05). The incidence of poly-drug resistant tuberculosis (PDR-TB) in TB-no DM patients (10.70 vs. 2.63%, OR: 4.43; 95% CI, 1.07–18.36) was higher than that in the TB-DM group (P< 0.05). In univariate and multivariate analysis, none of the basic factors were statistically significant with drug resistance among TB-DM cases (allP> 0.05). Retreatment was the risk factor of drug resistance among TB-no DM cases.

Conclusions

Our results showed that the drug resistance rate of the TB-DM group was not higher than that of the TB-no DM group. Patients with TB-no DM were at a higher risk for PDR-TB, but not for MDR-TB, MR-TB, and drug resistance-TB. Special attention should be paid to TB-no DM patients who have been previously treated. In the future, large-scale and well-designed prospective studies are needed to clarify the impact of DM on the drug-resistance among TB.
Associations Between Uncertainty Stress, Life Stress and Internet Addiction Among Medical Students
Authors: Yang Qian , Wu Zhihua , Yang Xiaozhao , Jiang Shuhan , Wu Dan , Oliffe John L.
Published: January 2022

Objectives

Internet Addiction (IA) is a growing issue predominate in adolescents and young adults. Although the effects of diverse stressors on IA have been highlighted, there is little consensus about the specific underpinnings of IA. The current study aims to investigate associations between uncertainty stress, life stress and IA among Chinese university medical students.

Methods

A cross-sectional survey employing multi-stage sampling was used. Data were collected from 6,061 students from 27 university medical programs across China. Associations between uncertainty stress, life stress, and IA were examined by means of multivariate logistic regression.

Results

The findings indicated that the overall IA prevalence was 12.6% (95% CI 11.7-13.5), life stress prevalence was 8.1% (95% CI 7.4-8.8), and uncertainty stress prevalence was 19.1% (95% CI 18.1-20.1). Multivariate logistic regression showed that uncertainty stress [adjusted OR 2.60 (95% CI 2.14-3.15), P < 0.001] and life stress [adjusted OR 1.71 (95% CI 1.32-2.23), P < 0.001] were positively associated with IA. Population Attributable Risk (PAR) of uncertainty stress associated with IA was 29%, and that of life stress was 15%.

Conclusions

The contribution of uncertainty stress to IA is significantly higher than that of life stress. High uncertainty stress, being male and born in a region of higher economic status were associated with excessive Internet use and IA.
Prevalence of Metabolic Syndrome and Associated Risk Factors in the United Arab Emirates: A Cross-Sectional Population-Based Study
Authors: Mahmoud Ibrahim , Sulaiman Nabil
Published: January 2022

Aims

To estimate the prevalence of metabolic syndrome (MetS) and its associated risk factors among the United Arab Emirates (UAE) residents.

Methods

A cross-sectional population-based study was conducted among adults living in Sharjah and Northern Emirates using the UAE National Diabetes and Lifestyle Study (UAEDIAB) data. Anthropometric measurements and fasting blood samples were obtained. The National Cholesterol Education Program's Adult Treatment Panel III (NCEP/ATP III) guidelines were used to define metabolic syndrome (MetS).

Results

A total of 3,212 subjects (74.1% men, 25.9% women, mean age 39 ± 11.3 years old) were included in this study. The overall prevalence of MetS was 37.4% (32.7% in women and 39% in men). The prevalence was 33.6% in the Emirati population (38.7% in women and 28.8% in men), 34.5% in the Arab non-Emirati population (29.8% in women and 36.3% in men) and 40.7% in the Asian non-Arab population (25.8% in women and 43.1% in men). Age, gender, ethnicity, educational level, marital status and body mass index were positively associated with MetS.

Conclusions

This study indicates a high burden of MetS in the UAE, particularly among Emirati women and Asian non-Arab men. The young adult population in the UAE has a high prevalence of MetS compared to global estimates in the same age group. Aggressive intervention strategies targeting the whole population as well as individuals at a high risk are recommended to prevent the development of cardiovascular diseases.
Quantity and Quality of Healthcare Professionals, Transfer Delay and In-hospital Mortality Among ST-Segment Elevation Myocardial Infarction: A Mixed-Method Cross-Sectional Study of 89 Emergency Medical Stations in China
Authors: Zhou Qiang , Tian Wenya , Wu Rengyu , Qin Chongzhen , Zhang Hongjuan , Zhang Haiyan , Zhou Shuduo , Li Siwen , Jin Yinzi , Zheng Zhi-Jie
Published: January 2022

Background

Transfer delay provokes prolongation of prehospital time, which contributes to treatment delay that endangers patients with ST-segment elevation myocardial infarction (STEMI). A key constraint in reducing transfer delay is the shortage of emergency healthcare workers. This study was to explore the influence of the quality and quantity of healthcare professionals at emergency medical stations on transfer delay and in-hospital mortality among STEMI patients.

Methods

A cross-sectional study using mixed methods was conducted at 89 emergency stations in 9 districts in China's Shenzhen province. Based on a sample of 31 hospitals, 1,255 healthcare professionals, and 3,131 patients with STEMI, a generalized linear model was used to explore the associations between the quality and quantity of healthcare professionals and transfer delay and in-hospital mortality among STEMI patients. Qualitative data were collected and analyzed to explore the reasons for the lack of qualified healthcare professionals at emergency medical stations.

Results

The analysis of the quantity of healthcare professionals showed that an increase of one physician per 100,000 individuals was associated with decreased transfer delay for patients with STEMI by 5.087 min (95% CI −6.722, −3.452; P < 0.001). An increase of one nurse per 100,000 individuals was associated with decreased transfer delay by 1.471 min (95% CI −2.943, 0.002; P=0.050). Analysis of the quality of healthcare professionals showed that an increase of one physician with an undergraduate degree per 100,000 individuals was associated with decreased transfer delay for patients with STEMI by 8.508 min (95% CI −10.457, −6.558; P < 0.001). An increase of one nurse with an undergraduate degree per 100,000 individuals was associated with decreased transfer delay by 6.645 min (95% CI −8.218, −5.072; P < 0.001). Qualitative analysis illustrated that the main reasons for low satisfaction of healthcare professionals at emergency medical stations included low income, limited promotion opportunities, and poor working environment.

Conclusions

The quantity and quality of emergency healthcare professionals are key factors influencing transfer delay in STEMI patients. The government should increase the quantity of healthcare professionals at emergency medical stations, strengthen the training, and improve their performance by linking with clinical pathways to enhance job enthusiasm among emergency healthcare professionals.
Implementation of Best-Evidence Osteoarthritis Care: Perspectives on Challenges for, and Opportunities From, Low and Middle-Income Countries
Authors: Eyles Jillian P. , Sharma Saurab , Telles Rosa Weiss , Namane Mosedi , Hunter David J. , Bowden Jocelyn L.
Published: January 2022

The “Joint Effort Initiative” (JEI) is an international consortium of clinicians, researchers, and consumers under the auspices of the Osteoarthritis Research Society International (OARSI). The JEI was formed with a vision to improve the implementation of coordinated programs of best evidence osteoarthritis care globally. To better understand some of the issues around osteoarthritis care in low- and middle-income countries (LMICs), the JEI invited clinician researcher representatives from South Africa, Brazil, and Nepal to discuss their perspectives on challenges and opportunities to implementing best-evidence osteoarthritis care at the OARSI World Pre-Congress Workshop. We summarize and discuss the main themes of the presentations in this paper. The challenges to implementing evidence-based osteoarthritis care identified in LMICs include health inequities, unaffordability of osteoarthritis management and the failure to recognize osteoarthritis as an important disease. Fragmented healthcare services and a lack of health professional knowledge and skills are also important factors affecting osteoarthritis care in LMICs. We discuss considerations for developing strategies to improve osteoarthritis care in LMICs. Existing opportunities may be leveraged to facilitate the implementation of best-evidence osteoarthritis care. We also discuss strategies to support the implementation, such as the provision of high-quality healthcare professional and consumer education, and systemic healthcare reforms.
Diet-Induced Non-alcoholic Fatty Liver Disease and Associated Gut Dysbiosis Are Exacerbated by Oral Infection
Authors: Simas Alexandra M. , Kramer Carolyn D. , Genco Caroline A.
Published: January 2022

Increasing evidence indicates that chronic inflammation due to periodontal disease is associated with progression of non-alcoholic fatty liver disease (NAFLD) caused by a Western diet. NAFLD has also been associated with oral infection with the etiological agent of periodontal disease, Porphyromonas gingivalis. P. gingivalis oral infection has been shown to induce cardiometabolic disease features including hepatic lipid accumulation while also leading to dysbiosis of the gut microbiome. However, the impact of P. gingivalis infection on the gut microbiota of mice with diet-induced NAFLD and the potential for those changes to mediate NAFLD progression has yet to be determined. In the current study, we have demonstrated that P. gingivalis infection induced sustained alterations of the gut microbiota composition and predicted functions, which was associated with the promotion of NAFLD in steatotic mice. Reduced abundance of short-chain fatty acid-producing microbiota was observed after both acute and chronic P. gingivalis infection. Collectively, our findings demonstrate that P. gingivalis infection produces a persistent change in the gut microbiota composition and predicted functions that promotes steatosis and metabolic disease.
Loop Quantum Cosmological Model From ADM Hamiltonian
Authors: Ding You , Zhang Xiangdong
Published: January 2022

The loop quantum cosmological model from ADM Hamiltonian is studied in this article. We consider the spatially flat homogeneous FRW model. It turns out that the modified Friedmann equation keeps the same form as the APS LQC model. However, the critical matter density for the bounce point is only a quarter of the previous APS model, that is, ρcL=ρc4. This is interesting because the lower critical bounce density means the quantum gravity effects will get involved earlier than the previous LQC model. Besides, the lower critical density also means the detection of quantum gravity effects easier than the previous model.
Profit Efficiency of Smallholder Vegetable Farms in Nepal: Implications for Improving Household Income
Authors: Shrestha Rudra Bahadur , Bhandari Humnath , Pandey Sushil
Published: January 2022

Enhancing profit efficiency in vegetable farming is important to increase income, livelihoods, and nutrition security, and to reduce poverty of smallholder farmers, particularly in developing countries. This study examined the profit efficiency and its determinants in smallholder vegetable farms in Nepal using the stochastic translog profit function with cross-section data collected in 2013. The results revealed a high level of inefficiency in vegetable farms because of the combined effects of technical, allocative, and scale inefficiencies. The profitability differential in vegetable farms is significantly explained by input variables, namely, labor, land, seeds, fertilizer, pesticides, and capital. The determinants of profit inefficiency in vegetable farming were the types of crop varieties, access to information and extension services, access to agricultural credit, distance of farms to markets, and sex of farm manager. The profit efficiency in vegetable farming can be enhanced by adopting improved seed varieties, improving effective information and extension programs, increasing accessibility of credit facilities to the farmers, developing market infrastructure, and empowering women farmers in vegetable farming that leads to improve household income and nutrition security.
Risk Factors Analysis of Thoracic Trauma Complicated With Acute Respiratory Distress Syndrome and Observation of Curative Effect of Lung-Protective Ventilation
Authors: Ma Xiaoyu , Dong Zefang , Wang Yusuo , Gu Peidong , Fang Jinghua , Gao Shaolin
Published: January 2022

Purpose

To explore the risk factors of acute respiratory distress syndrome (ARDS) secondary to thoracic trauma and the therapeutic effect of protective lung ventilation in patients with acute respiratory distress syndrome complicated with thoracic trauma.

Methods

We collected 206 patients with thoracic trauma admitted to our hospital from September 2017 to March 2021, counted the incidence of ARDS and analyzed the risk factors of ARDS. To observe the clinical efficacy of the application of lung-protective ventilation therapy in patients with thoracic trauma combined with ARDS.

Results

Among 206 patients with thoracic trauma, there were 82 cases of combined ARDS, and its incidence was 39.81%. The 82 patients with ARDS were randomly divided into the control group and the observation group with 42 cases each, and different ventilation methods were used for treatment. The results showed that the mechanical ventilation time (MVT) was shorter in the observation group than in the control group, and the incidence of ventilator-associated lung injury (VALI) and case fatality rate (CFR) were lower than those in the control group (P < 0.05). Arterial partial pressure of oxygen (Pa02), arterial blood carbon dioxide partial pressure (PaCO2), and Oxygenation index (arterial partial pressure of oxygen/Fraction of inspiration O2, PaO2/FiO2) were significantly improved better in both groups after treatment; compared with the control group, patients in the observation group had higher Pa02 levels and lower PaCO2 levels at 8 h and 24 h after ventilation (P < 0.05). Multivariate analysis revealed that blunt trauma, massive blood transfusion, procalcitonin (PCT) level, tumor necrosis factor-α (TNF-α) level, and acute physiology and chronic health score (APACHE II) were all risk factors for Thoracic trauma with ARDS.

Conclusion

Risk factors for the development of ARDS after thoracic trauma are blunt injuries, massive blood transfusion, high PCT and TNF-α levels, and high APACHE II scores, which can be given active interventions in the early stage of clinical practice to improve patient prognosis. The use of protective lung ventilation therapy can improve the clinical outcome of patients with thoracic trauma combined with ARDS, which is important for improving the ventilation effect and respiratory function of patients.
Characterization of the Gut Microbiome and Resistomes of Wild and Zoo-Captive Macaques
Authors: Jia Ting , Chang Wei-Shan , Marcelino Vanessa R. , Zhao Sufen , Liu Xuefeng , You Yuyan , Holmes Edward C. , Shi Mang , Zhang Chenglin
Published: January 2022

Rhesus macaques (Macaca mulatta) are the most widely distributed species of Old World monkey and are frequently used as animal models to study human health and disease. Their gastrointestinal microbial community likely plays a major role in their physiology, ecology and evolution. Herein, we compared the fecal microbiome and antibiotic resistance genes in 15 free-ranging and 81 zoo-captive rhesus macaques sampled from two zoos in China, using both 16S amplicon sequencing and whole genome shotgun DNA sequencing approaches. Our data revealed similar levels of microbial diversity/richness among the three groups, although the composition of each group differed significantly and were particularly marked between the two zoo-captive and one wild groups. Zoo-captive animals also demonstrated a greater abundance and diversity of antibiotic genes. Through whole genome shotgun sequencing we also identified a mammalian (simian) associated adenovirus. Overall, this study provides a comprehensive analysis of resistomes and microbiomes in zoo-captive and free-ranging monkeys, revealing that semi-captive wildlife might harbor a higher diversity of antimicrobial resistant genes.
Comprehensive Analysis of Long Non-coding RNA and mRNA Transcriptomes Related to Hypoxia Adaptation in Tibetan Sheep
Authors: Lu Zengkui , Yuan Chao , Li Jianye , Guo Tingting , Yue Yaojing , Niu Chune , Liu Jianbin , Yang Bohui
Published: January 2022

Tibetan sheep have lived on the Qinghai-Tibet Plateau for a long time, and after long-term natural selection, they have shown stable genetic adaptability to high-altitude environments. However, little is known about the molecular mechanisms of the long non-coding (lnc)RNAs involved in the adaptation of Tibetan sheep to hypoxia. Here, we collected lung tissues from high-altitude Tibetan sheep and low-altitude Hu sheep for RNA sequencing to study the regulatory mechanisms of the lncRNAs and mRNAs in the adaptation of Tibetan sheep to hypoxia. We identified 254 differentially expressed lncRNAs and 1,502 differentially expressed mRNAs. We found 20 pairs of cis-regulatory relationships between 15 differentially expressed lncRNAs and 14 protein-coding genes and two pairs of trans-regulatory relationships between two differentially expressed lncRNAs and two protein-coding genes. These differentially expressed mRNAs and lncRNA target genes were mainly enriched in pathways related to lipid metabolism and immune function. Interaction network analysis showed that 17 differentially expressed lncRNAs and 15 differentially expressed mRNAs had an interactive relationship. Additionally, we used six differentially expressed lncRNAs and mRNAs to verify the accuracy of the sequencing data via qRT-PCR. Our results provide a comprehensive overview of the expression patterns of the lncRNAs and mRNAs involved in the adaptation of Tibetan sheep to hypoxia, laying a foundation for further analysis of the adaptations of plateau animals.
Curcumin Supplementation Protects Broiler Chickens Against the Renal Oxidative Stress Induced by the Dietary Exposure to Low Levels of Aflatoxin B1
Authors: Damiano Sara , Jarriyawattanachaikul Watanya , Girolami Flavia , Longobardi Consiglia , Nebbia Carlo , Andretta Emanuela , Lauritano Chiara , Dabbou Sihem , Avantaggiato Giuseppina , Schiavone Achille , Badino Paola , Ciarcia Roberto
Published: January 2022

Aflatoxin B1 (AFB1) causes hepatotoxicity, immunotoxicity, and kidney damage, and it is included in group I of human carcinogens. The European Commission has established maximum limits of AFB1 in feed, ranging from 5 to 20 μg/kg. Chicken is moderately sensitive to AFB1, which results in reduced growth performance and economic losses. Oxidative stress triggered by AFB1 plays a crucial role in kidney damage and the antioxidant activity of Curcumin (CURC) could help in preventing such adverse effect. Twenty-days-old broilers were treated for 10 days with AFB1 (0.02 mg/kg feed), alone or in combination with CURC (400 mg/kg feed), to explore the effects on the renal tissue. Animals exposed to AFB1 alone displayed alterations of the oxidative stress parameters compared with controls: serum antioxidant capacity, and enzymatic activity of kidney superoxide dismutase, catalase and glutathione peroxidase were decreased, while renal malondialdehyde levels and NADPH oxidase complex expression were increased. The administration of CURC attenuates all the oxidative stress parameters modified by AFB1 in the chicken kidney, opening new perspectives in the management of aflatoxicosis.
Field Trial of Factors Associated With the Presence of Dead and Non-ambulatory Pigs During Transport Across Three Colombian Slaughterhouses
Authors: Romero Marlyn H. , Sánchez Jorge Alberto , Hernandez Rick Obrian
Published: January 2022

Transport by land is an essential component for the commercialization of fattening pigs and can have a negative impact on animal welfare. In slaughterhouses, the presence of dead and non-ambulatory animals is an indicator of poor welfare during transport. The objective of the study was to identify risk factors associated with the frequency of dead and non-ambulatory pigs during transport. A survey was conducted in three Colombian slaughterhouses. Data were collected from 372 batches (n = 18,437 gilts barrows) and transported directly from the farms to the slaughterhouses. Each truck was individually evaluated; a structured survey was administered to drivers, non-ambulatory and dead pigs on arrival were identified and blood samples were obtained from non-ambulatory pigs to assess physiological indicators of stress. Mortality rates per batch at arrival ranged from 0.08 to 0.17% and prevalence of non-ambulatory pigs per batch ranged from 0.84 to 1.37%.The results of the multilevel mixed effects linear regression model identified the following as risk factors associated with the frequency of total transport losses: truck speed (P = 0.04), distance (P < 0.01), transport time (P < 0.01), load size (P < 0.01) and the driver (P < 0.01) including the farm as a fixed effect. This study identified risk factors that increased the probability of total transport losses during land transport under Colombian commercial conditions. But more research that involves commercial drivers is needed to develop effective strategies to improve Colombian pig's transportation chain.
Effect of Silicon-Containing Fertilizers on the Nutritional Value of Grass–Legume Mixtures on Temporary Grasslands
Authors: Borawska-Jarmułowicz Barbara , Mastalerczuk Grażyna , Janicka Maria , Wróbel Barbara
Published: January 2022

The study was conducted to evaluate the influence of silicon application on the botanical composition of grass–legume mixtures as well as the nutritional value of individual species and mixtures. The two-factor field experiment was established in a split-block arrangement. The two grass–legume mixtures, consisting of Dactylis glomerata, Festulolium braunii and Trifolium pratense or Medicago x varia and grass mixture—Dactylis glomerata, Festulolium braunii and Lolium perenne, were sown on arable land. Silicon foliar and mineral NPK fertilization was applied in three variants: NPK (non-Si-fertilized), NPK+Si (Herbagreen), NPK+Si (Optysil). Changes in the botanical composition in the sward of mixtures during the study period were influenced by the competitiveness of individual plants and weather conditions, whereas the effect of foliar fertilization with silicon was only slightly marked. The nutritional value of individual species of grasses and legumes varied over the years and also depended on the application of silicon. The greatest content of crude protein (CP), irrespective of the species and variant of silicon fertilization, was noticed in years with no drought. Our research confirmed the beneficial effect of silicon fertilization, independent of weather conditions, on the CP content and on the reduction of crude fibre (CF), neutral detergent fibre (NDF) and acid detergent fibre (ADF) in plants, especially when Herbagreen was applied. The nutritional value of the mixtures under applied silicon fertilization depended on botanical composition. In the conditions of low rainfall, a large share of F. braunii and an approximately 20% contribution of T. pratense resulted in a higher content of CP. These results may be used in the fertilization practices of temporary grassland for enhancing nutritional value, especially crude protein content, and on the reduction of fibre in the sward.
The Agro-Climatic Change Characteristics across China during the Latest Decades
Authors: Huo Zhiguo , Zhang Lei , Kong Rui , Jiang Mengyuan , Zhang Haiyan
Published: January 2022

Climate change has been highlighted over the last decades worldwide, with pronounced higher warming trends for China. Induced by climate change, to some extent, agricultural production has changed, as well as the climatic resources during the agricultural growing season. An obvious longer potential agricultural growing season (PAGS) was detected in the latest decades from 1961–2020. The spatial–temporal characteristics of change and variation of climatic resources during the PAGS were explored, utilizing comparisons of the differences in mean magnitude, standard deviation, and trends. In the period of 1991–2020 relative to 1961–1990, alterations in PAGS were characterized by increases of 0–1.5 °C in mean air temperature overall, 0.8–23.8% increases in precipitation in the southeast, northeast, and west, as well as a decrease of 2.1–10.2% in insolation in central-south regions but an increase of 0.3–6.7% in the north and west. The features were pronounced during the PAGS in the primary agricultural zones as follows: (1) Northeast China, increasing and stable temperature but unstable precipitation and insolation; (2) North, eastern-west, and Southwest China, increasing but unstable temperature, decreasing but stable precipitation as well as decreasing and unstable insolation; (3) Southeast China, increasing but unstable temperature and precipitation as well as decreasing and unstable insolation; (4) West China, increasing but unstable temperature and precipitation as well as increasing but unstable insolation. Further comparisons between agro-climatic change and climate change indicated that temperature alterations during the PAGS were less numerous while precipitation and insolation were more unstable than the changes during the whole year. These findings can assist the understanding of regional agricultural climate changes and guide agricultural production practices in response.
Yellow Mealworm Composition after Convective and Freeze Drying—Preliminary Results
Authors: Krzyżaniak Michał , Aljewicz Marek , Bordiean Anna , Stolarski Mariusz Jerzy
Published: January 2022

Insects are a potential source of food and feed for humans and livestock, and they can be consumed in different forms. A combination of freezing, blanching, and drying methods are often recommended to prolong the shelf life of insect-based foods and reduce their microbial loads. However, these processes affect the quality of the end product. Therefore, the aim of this study was to determine the extent to which various drying methods influence the selected physical and chemical parameters of yellow mealworm (Tenebrio molitor L.) larvae. Insects were blanched (for 60 and 120 s) and dried with the use of two methods: convective drying (at a temperature of 60 °C and 80 °C) and freeze-drying (−30 °C) for 12, 16 or 24 h. Blanched and dried insects were subjected to laboratory analyses. The examined samples were characterized by low moisture content in the range of 3.15% to 5.47%, and they differed considerably in water activity (0.06–0.55). Moisture content and water activity were substantially higher in larvae blanched for 60 s and freeze-dried than in larvae dried with the use of the remaining methods. However, no significant changes in the protein, fiber, or fat content of insects were found when drying parameters were modified. Therefore, nutritional composition and microbial loads in dried mealworm larvae should be investigated in the future.