bluuebunny/crossref_metadata_2025 huggingface dataset:
Critical period of grass weed control in ALS-tolerant grain sorghum (Sorghum bicolor) is affected by planting date and environment
Authors: Contreras Diego , Leon Ramon G. , Post Angela R. , Everman Wesley J.
Published: September 2022

Field experiments were conducted at two locations in North Carolina (Clayton and Rocky Mount) to determine the influence of row spacing and planting date on the critical period of weed control (CPWC) of grass weeds in ALS-tolerant grain. Grain sorghum was planted in May and June 2019, with either a 38 or 91 cm row spacing using an ALS-tolerant sorghum variety. Treatments consisted of “weedy” or “weed-free” plots up to 2, 3, 5, and 7 weeks after crop planting (WAP) and two control treatments of weedy and weed-free all season. Selection of grass weed species was achieved by controlling broadleaf weeds with a premix of bromoxynil plus pyrasulfutole at a rate of 264 g a.i. ha-1.Grass weeds were controlled using nicosulfuron at a rate of 69 g of a.i. ha-1. The CPWC was significantly different across locations. Row spacing and planting date factors did not influence the CPWC at Clayton. Planting date was a significant factor for the CPWC at Rocky Mount, however row spacing did not have any effect on the CPWC. Results for the CPWC are presented in terms growing degree days (GDD) from the date of crop sowing. The CPWC for grass weeds in grain sorghum at Clayton was from 368 to 849 GDD. The CPWC at Rocky Mount for May-planted grain sorghum was from 405 to 876 GDD, while the CPWC for June-planted grain sorghum ranged from 228 to 1042 GDD. These results demonstrate that cultural and environmental factors may influence the beginning, duration and end of the CPWC.
Correlation analysis of financial assets based on asymmetric copula
Authors: Li Xia , Hou Bing
Published: September 2022

Based on the asymmetric copula function, this paper analyzes the static and dynamic correlation between Shanghai Composite Index and Shenzhen Composite Index. Through the static analysis, it is found that the asymmetric copula function is better than Gumbel Copula in describing the distribution characteristics of the top tail dependence between the Shanghai Composite Index and the Shenzhen Composite Index, and the copula correlation coefficient definition based on the asymmetric copula function can well describe the asymmetric dependence between variables. In the time-varying analysis, the paper improves the traditional dynamic evolution equation of the tail-dependence coefficient. Through empirical analysis, the result shows that the improved dynamic evolution equation can better reflect the dynamic evolution process of the tail-dependence coefficient.
Hydrogel co-loading SO2 prodrug and FeGA nanoparticles for enhancing chemodynamic therapy by photothermal-triggered SO2 gas therapy
Authors: Huang Qinqin , Lyu Meng , Tang Wenxue , Qi Pengyuan , Hu Hongzhi
Published: September 2022

Chemodynamic therapy (CDT) is an effective anti-tumor method, while CDT alone cannot achieve a good therapeutic effect. Moreover, the overexpression of glutathione (GSH) in tumor cells dramatically limits the efficiency of CDT. Here, we proposed a hydrogel co-loading SO2 prodrug and FeGA nanoparticles (NPs) for enhancing CDT by photothermal-triggered SO2 gas therapy (FBH) system by mixing benzothiazolyl sulfonates (BTS) and FeGA NPs in a certain ratio and encapsulating them in a heat-sensitive hydrogel. FeGA NPs could accelerate the release of Fe2+ under acidic conditions and light, and combine with excess H2O2 in the tumor for chemokinetic treatment. BTS, as a water-soluble prodrug of SO2, can accurately control the release of SO2 gas by virtue of the excellent photothermal conversion ability of FeGA NPs and the acidic pH value of tumor site. SO2 can not only induce cell apoptosis, but also consume excess GSH in cancer cells and increase the content of reactive oxygen species, which seriously destroyed the redox balance in cancer cells and further promotes the therapeutic effect of Fenton reaction. The intelligent FBH system provided a new approach for the synergistic treatment of CDT and SO2 gas, which demonstrated good anticancer effects both in vivo and in vitro.
High performance bio-supercapacitor electrodes composed of graphitized hemicellulose porous carbon spheres
Authors: Zhang Zhili , Li Fengfeng , Chen Jiachuan , Yang Guihua , Ji Xingxiang , Tian Zhongjian , Wang Baobin , Zhang Lei , Lucia Lucian
Published: September 2022

A template-free and one-step carbonization process was developed for fabricating graphitic porous carbon spheres (GPCSs) on hemicelluloses as the electrode material for supercapacitors. This method is green, low-energy, and less time consuming compared to the conventional two-step process (pore-forming and graphitizing). It uses K2FeO4, a mild activating agent that fulfills synchronous activation and graphitization. The GPCSs is regular spherical shape, have high nanoporosity, a large specific surface area (1,250 m2 g−1), and have a high graphitization degree. A unique structural advantage includes a rich interconnected conductive network for electron transfer that shortens the ion transport distance of the electrolyte. Remarkably, the GPCSs electrode displays outstanding electrochemical performance including high specific capacitance (262 F g−1 at 1.0 A g−1), rate capability energy (80%, 20 A g−1), and excellent cycling stability (95%, 10,000 cycles). This work represents a powerful methodology to develop sustainable and low-cost energy storage devices from hemicellulose.
Exploring the relationship between bearing extrusion and postoperative persistent pain in Oxford unicompartmental knee arthroplasty: A trajectory measurement study
Authors: Wen Pengfei , Zhang Qidong , Sun Xiaowei , Zhang Binfei , Ma Tao , Zhang Yumin
Published: September 2022

Objective: The aim of the study is to explore the relationship between the extrusion of the meniscus bearing and postoperative persistent pain of Oxford unicompartmental knee arthroplasty.

Methods: Patients undertaking Oxford UKA from January 2019 to June 2020 were retrospectively analyzed. Intraoperatively, the displacement and movement trajectory of the meniscus bearing was recorded by the specially designed gridding mold of the tibial component. The k-means clustering analysis was applied based on the incidence of postoperative persistent knee pain and the bearing extrusion distance. The intraoperative meniscus bearing movement trajectories were analyzed between the two groups and the patients’ clinical outcomes and radiographic assessments.

Results: The k-means clustering analysis indicated that the extrusion of the bearing of 5 mm was the grouping standard. There were 27 patients with 30 knees in the extrusion group and 58 patients with 68 knees in the non-extrusion group. The proportion of optimal bearing movement trajectories in the extrusion group was significantly lower than that in the non-extrusion group (p < 0.05). Postoperative persistent knee pain occurred in six cases (6.1%), with four and two cases in the extrusion and non-extrusion groups, respectively. The incidence of postoperative persistent knee pain in the extrusion group was higher than that of the non-extrusion group (p < 0.05). Radiographic assessment showed that the continuity of the femoral and tibial components in the extrusion group was greater than that in the non-extrusion group (p < 0.05). However, there were no differences in pre- and postoperative HKAA, the varus/valgus degree of both femoral and tibial components, and the flexion/extension angles of the femoral component, and the tibial slope also showed no statistical difference (p > 0.05).

Conclusion: For Oxford mobile-bearing UKA, the extrusion of meniscus bearing over 5 mm may increase the incidence of postoperative persistent knee pain, while the improvement of the bearing movement trajectory can effectively reduce this complication.
Glucose-activatable insulin delivery with charge-conversional polyelectrolyte multilayers for diabetes care
Authors: Yang Yanguang , Wang Xiangqian , Yuan Xiaopeng , Zhu Qiwei , Chen Shusen , Xia Donglin
Published: September 2022

One of the most effective treatments for diabetes is to design a glucose-regulated insulin (INS) delivery system that could adjust the INS release time and rate to reduce diabetes-related complications. Here, mixed multiple layer-by-layer (mmLbL)-INS microspheres were developed for glucose-mediated INS release and an enhanced hypoglycemic effect for diabetes care. To achieve ultrafast glucose-activated INS release, glucose oxidase (GOx) was assembled with a positively charged polymer and modified on INS LbL. The mmLbL-INS microspheres were constructed with one, two, and four layers of the polyelectrolyte LbL assembly at a ratio of 1:1:1. Under hyperglycemia, GOx converts a change in the hyperglycemic environment to a pH stimulus, thus providing sufficient hydrogen ion. The accumulated hydrogen ion starts LbL charge shifting, and anionic polymers are converted to cationic polymers through hydrolytic cleavage of amine-functionalized side chains. The results of in vitro INS release suggested that glucose can modulate the mmLbL-INS microspheres in a pulsatile profile. In vivo studies validated that this formulation enhanced the hypoglycemic effect in STZ-induced diabetic rats within 2 h of subcutaneous administration and facilitated stabilization of blood glucose levels for up to 2 days. This glucose-activatable LbL microsphere system could serve as a powerful tool for constructing a precisely controlled release system.
Evaluation of crack propagation in concrete bridges from vehicle-mounted camera images using deep learning and image processing
Authors: Nomura Yasutoshi , Inoue Masaya , Furuta Hitoshi
Published: September 2022

In Japan, all bridges should be inspected every 5 years. Usually, the inspection has been performed through the visual evaluation of experienced engineers. However, it requires a lot of load and expense. In order to reduce the inspection work, an attempt is made in this paper to develop a new inspection method using deep learning and image processing technologies. While using the photos obtained by vehicle-mounted camera, the damage states of bridges can be evaluated manually, it still requires a lot of time and load. To save the time and load, deep learning, which is a method of artificial intelligence is introduced. For image processing, it is necessary to utilize such pre-processing techniques as binarization of pictures and morphology treatment. To illustrate the applicability of the method developed here, some experiments are conducted by using the photos of running surface of concrete bridges of a monorail took by vehicle-mounted camera.
How solvent determines the molecular reactive conformation and the selectivity: Solvation spheres and energy
Authors: Hernández-Lima Joseelyne , Ramírez-Gualito Karla , Quiroz-García Beatriz , Silva-Portillo Ana Luisa , Carrillo-Nava Ernesto , Cortés-Guzmán Fernando
Published: September 2022

In solution, the solvent determines the molecular conformation and the chemical reaction viability and selectivity. When solvent-solute and solvent-solvent interactions present similar strengths, explicit salvation is the best way to describe a system. The problem to solve is how big the explicit shell should be. In this paper, we want to answer one of the fundamental questions in the implementation of explicit solvation, exactly how many solvent molecules should be added and where they should be placed. Here we determine the first solvent sphere around a molecule and describe how it controls the conformation and selectivity of a selected reaction. NMR experiments were carried out to identify the number of solvent molecules around the solute that constitutes the first solvent sphere, and the interaction between this solvent sphere and the solute was detected using DFT and QTAIM calculations. A new approach to the solvation energy is presented. Finally, we established the role of solvent molecules in the conformation of the solute and in the transition states that produce the two possible products of the reaction.
Engineering techniques to dendrite free Zinc-based rechargeable batteries
Authors: Worku Ababay Ketema
Published: September 2022

Rechargeable Zn-based batteries (RZBs) have garnered a great interest and are thought to be among the most promising options for next-generation energy storage technologies due to their low price, high levels of safety, adequate energy density and environmental friendliness. However, dendrite formation during stripping/plating prevents rechargeable zinc-based batteries from being used in real-world applications. Dendrite formation is still a concern, despite the fact that inhibitory strategies have been put up recently to eliminate the harmful effects of zinc dendrites. Thus, in order to direct the strategies for inhibiting zinc dendrite growth, it is vital to understand the formation mechanism of zinc dendrites. Hence, for the practical application of zinc-based batteries, is essential to use techniques that effectively prevent the creation and growth of zinc dendrites. The development and growth principles of zinc dendrites are first made clear in this review. The recent advances of solutions to the zinc dendrite problem are then discussed, including strategies to prevent dendrite growth and subsequent creation as much as possible, reduce the negative impacts of dendrites, and create dendrite-free deposition processes. Finally, the challenges and perspective for the development of zinc-based batteries are discussed.
Carbon effect calculation and upgrading strategy of agricultural land consolidation project in urban edge of Three Gorges Reservoir Area
Authors: Yang Wei , Li Xiaohua , Li Weihua , Zhang Yutao , Zhang Haizhen , Ran Yuhe
Published: September 2022

Under the background of promoting the construction of ecological civilization and the goal of carbon peak and carbon neutralization, it is of great significance to explore the measurement method and improvement strategy of the carbon effect of agricultural land consolidation. Based on a quantitative analysis and the whole life cycle of land consolidation, this study constructed a carbon effect accounting and analysis framework of agricultural land consolidation project from three stages of project initiation and design, project implementation, and operation management. Taking the agricultural land consolidation project in the Shiyan town on the urban edge of the Three Gorges Reservoir Area as a case, this study made empirical analysis and calculation and analyzed the carbon effect and influencing factors in different consolidation stages. The results showed that the overall carbon effect in the project area was a carbon source. The net carbon emission generated by the project construction was 8358t, which was mainly from workers input and concrete carbon emission; the carbon storage brought about by the adjustment of land use structure was 2,378.20t, which mainly came from the carbon storage increment of newly cultivated land; the carbon storage generated by the agricultural ecosystem was 1,100.04t, which was mainly based on the increase of cultivated land and the improvement of cultivated land quality; the carbon emission from agricultural production activities was 18.18t. Research conclusions: ① the carbon source effect of engineering construction is obvious. Artificial input and concrete are the main carbon sources in the hilly area at the edge of the metropolis; ② the adjustment of land use structure is manifested as a carbon sink effect, which mainly comes from the contribution of carbon storage of newly increased cultivated land; ③ the carbon effect of project operation and management may be a carbon source in the short term, and the long-term effect should be exerted; and ④ based on the concept of whole life cycle, promoting ecological land consolidation, optimizing project design, reasonably arranging consolidation projects, and strengthening operation management are effective measures to improve the carbon effect of land consolidation projects, which are conducive to the realization of the “double carbon” goal.
The effects of different stress on intestinal mucosal barrier and intestinal microecology were discussed based on three typical animal models
Authors: Guo Junfeng , Lou Xiaokun , Gong Wenyan , Bian Jing , Liao Yuhan , Wu Qi , Jiao Qibin , Zhang Xingwei
Published: September 2022

Recent studies have revealed that the effect of intestinal microecological disorders on organismal physiology is not limited to the digestive system, which provides new perspectives for microecological studies and new ideas for clinical diagnosis and prevention of microecology-related diseases. Stress triggers impairment of intestinal mucosal barrier function, which could be duplicated by animal models. In this paper, pathological animal models with high prevalence and typical stressors—corresponding to three major stressors of external environmental factors, internal environmental factors, and social psychological factors, respectively exemplified by burns, intestinal ischemia-reperfusion injury (IIRI), and depression models—were selected. We summarized the construction and evaluation of these typical animal models and the effects of stress on the organism and intestinal barrier, as well as systematically discussed the effects of different stresses on the intestinal mucosal barrier and intestinal microecology.
Testing the TEBOTS model in self-threatening situations: The role of narratives in the face of ostracism and mortality
Authors: Rieger Diana , Schneider Frank M.
Published: September 2022

The TEBOTS model predicts that narratives are sought after more often in times of depletion. The present study aimed at expanding this idea by testing whether engagement with narratives is also intensified under self-threatening conditions. Further, we examined whether narratives can serve coping functions. In a 3(Threat: mortality salience vs. ostracism vs. control condition) × 2(Review of the narrative: positive vs. negative) online experiment (N= 228), we tested whether self-threats and the expectation towards the narrative increase entertainment experiences and facilitate self-serving attributions. The results demonstrated that self-threats and a positive review indeed increased the entertainment experience. Narratives could support coping with anexistentialthreat through enhancing self-serving attributions. The findings are discussed in light of the TEBOTS model and its application in the context of self-threats.
Piezo1 in vascular remodeling of atherosclerosis and pulmonary arterial hypertension: A potential therapeutic target
Authors: Xu Han , He Yu , Hong Tianying , Bi Cong , Li Jing , Xia Mingfeng
Published: September 2022

Vascular remodeling (VR) is a structural and functional change of blood vessels to adapt to the changes of internal and external environment. It is one of the common pathological features of many vascular proliferative diseases. The process of VR is mainly manifested in the changes of vascular wall structure and function, including intimal hyperplasia, thickening or thinning of media, fibrosis of adventitia, etc. These changes are also the pathological basis of aging and various cardiovascular diseases. Mechanical force is the basis of cardiovascular biomechanics, and the newly discovered mechanical sensitive ion channel Piezo1 is widely distributed in the whole cardiovascular system. Studies have confirmed that Piezo1, a mechanically sensitive ion channel, plays an important role in cardiovascular remodeling diseases. This article reviews the molecular mechanism of Piezo1 in atherosclerosis, hypertension and pulmonary hypertension, in order to provide a theoretical basis for the further study of vascular remodeling.
Left ventricular assist device bioinformatics identify possible hubgenes and regulatory networks involved in the myocardium of patients with left ventricular assist device
Authors: Ajmal Maryam , Ajmal Aisha , Rizvi Maryam , Salim Umar , Huang Lei
Published: September 2022

Objective

The aim of this study was to clarify the changes of myocardial gene expression profile after left ventricular assist device (LVAD) implantation and the related molecular biological significance.

Methods

A thorough bioinformatic analysis to evaluate the changes in gene expression profile in patients pre-LVAD and post-LVAD was conducted. Four relevant gene expression datasets—GSE430, GSE974, GSE21610, and GSE52601 from Gene Expression Omnibus (GEO) database were downloaded. Analysis of GEO2R, Gene Ontology (GO), protein-protein interaction (PPI) were used to determine differentially expressed genes (DEGs) and their function, respectively.

Results

A total of 37 DEGs were identified, including 26 down-regulated and 11 up-regulated genes. The molecular function of DEGs were enriched in “cytokine activity,” “neurotransmitter binding,” “receptor ligand activity.” The gene set enrichment analysis (GSEA) revealed an overall marked increase of neutrophil degranulation signaling, closely correlated with the G protein coupled receptor (GPCR)—ligand binding process after LVAD assistance. 16 hubgenes in these DEGs were further selected and the biological process involved is mainly related to positive regulation of leukocyte chemotaxis mediated by chemokines.

Conclusion

Inflammatory signaling pathway is crucial for the pathophysiology after LVAD implantation. Chemokines mediate cardiac inflammatory response and tissue remodeling after LVAD implantation through GPCR—ligand binding.
Sub-satisfactory recanalization of severe middle cerebral artery stenoses can significantly improve hemodynamics
Authors: Zhang Kun , Ren Wei , Li Tian-Xiao , Wang Zi-Liang , Gao Bu-Lang , Xia Jin-Chao , Gao Hui-Li , Wang Yong-Feng , Gu Jian-Jun
Published: September 2022

Purpose

To investigate the effect of sub-satisfactory stent recanalization on hemodynamic stresses for severe stenoses of the middle cerebral artery (MCA) M 1 segment.

Materials and methods

Patients with severe stenoses of the MCA M1 segment treated with endovascular stent angioplasty were retrospectively enrolled. Three-dimensional digital subtraction angiography before and after stenting was performed; the computational fluid dynamics (CFD) analysis of hemodynamic stresses at the stenosis and normal segments proximal and distal to the stenoses was analyzed.

Results

Fifty-one patients with severe stenosis at the MCA M1 segment were enrolled, with the stenosis length ranging from 5.1 to 12.8 mm (mean 9 ± 3.3 mm). Stent angioplasty was successful in all (100%) the patients. The angiography immediately after stenting demonstrated a significant (P < 0.05) decrease in MCA stenosis after comparison with before stenting (31.4 ±12.5% vs. 87.5 ± 9.6%), with residual stenosis of 15–30% (mean 22.4 ± 3.5%). Before stenting, the total pressure was significantly higher (P < 0.0001), while the WSS, velocity, and vorticity were all significantly decreased (P < 0.0001) at the normal arterial segment proximal to the stenosis, and the total pressure, WSS, velocity, and vorticity were all significantly decreased (P < 0.0001) at the normal arterial segment distal to the stenosis compared with those at the stenosis. After sub-satisfactory stenting recanalization, all the hemodynamic stresses proximal or distal to the stenosis and at the perforator root were improved compared with those before stenting and were similar to those after virtual stenosis removal.

Conclusion

Sub-satisfactory recanalization of severe MCA stenoses can significantly improve the hemodynamic status for cerebral perfusion at the stenoses.
Inflammatory cytokines are associated to lower glomerular filtration rate in patients with hypertensive crisis
Authors: Andrade Days O. , Aguiar Franciana L. , Mansor Ana Luiza P. , Valente Flavia M. , Souza Doroteia R. S. , Lopes Valquiria da Silva , Fernandes Leticia B. , Godoy Moacir F. , Yugar-Toledo Juan C. , Cosenso-Martin Luciana N. , Vilela-Martin Jose F.
Published: September 2022

Introduction

Hypertension and kidney function are closely related. However, there are few studies on renal function during acute elevation of blood pressure (BP), denominated hypertensive crisis (HC).

Objectives

To evaluate the relationship between renal function and inflammatory cytokines in HC, subdivided into hypertensive urgency (HUrg) and emergency (HEmerg).

Materials and methods

This cross-sectional study was carried out in 74 normotensive (NT) and 74 controlled hypertensive individuals (ContrHT) followed up in outpatient care. Additionally, 78 subjects with hypertensive emergency (HEmerg) and 50 in hypertensive urgency (HUrg), attended in emergency room, were also evaluated. Hypertensive crisis was classified into HEmerg, defined by systolic blood pressure (BP) ≥ 180 mmHg and/or diastolic BP ≥ 120 mmHg in presence of target-organ damage (TOD), and HypUrg, clinical situation with BP elevation without TOD. The glomerular filtration rate (eGFR) was estimated, and cytokine levels were measured. Statistical analysis was performed using the Kruskal-Wallis or Mann-Whitney test and Spearman’s correlation, with significant differences p-value < 0.05.

Results

The median age was 53.5 years in the NT group (52 female), 61 years in the ContrHT group (52 female), and 62.5 years in the HC group (63 female) (p-value < 0.0001). The median BP was 118.5/75 mmHg for NT, 113.5/71 for ContrHT, and 198.5/120 mmHg for HC, respectively (p-value < 0.0001 among groups). BP and heart rate levels were significantly higher in the HC group compared to the NT and ContrHT groups (P < 0.001 for all). The eGFR was significantly lower in HC group compared to the NT and ContrHT groups. The cytokine levels were higher in the HEmerg and HUrg groups compared to ContrHT group (P < 0.0001, except for IL-1β in HUrg vs. ContrHT), without difference between the acute elevation of BP groups. Thus, all cytokines were significantly elevated in patients with HC compared to the control groups (NT and ContrHT). There was a negative correlation between eGFR and the cytokines (IL-1β, IL-6, IL-8, IL-10, and TNF-α) in the HC group.

Conclusion

Elevated inflammatory cytokines are associated with reduced eGFR in individuals with HC compared to control groups, suggesting that the inflammatory process participates in the pathogenesis of acute elevations of BP.
Cardiovascular drug interventions in the cardio-oncology clinic by a cardiology pharmacist: ICOP-Pharm study
Authors: Yaseen Israa Fadhil , Farhan Hasan Ali
Published: September 2022

Background

Cardio-oncology is a rapidly growing field that requires a novel service design to deal with the increasing number of patients. It is reported that the volume of patients at the cardio-oncology clinic in the United Kingdom is 535 patients/5 years and in Canada is 779 patients/7 years. The pharmacist has a role in reducing the consultation time of physicians.

Objective

To identify the role of a qualified cardiology pharmacist at the cardio-oncology clinic using a new paradigm based on complementary interventions with the cardiologist for the management of patients with cancer and cardiovascular risk factors and/or cardiovascular diseases (CVRF/CVD).

Methods

A prospective observational study was conducted at the cardio-oncology clinic in the Medical City in Baghdad, Iraq between December 2020 and December 2021. Patients with CVRF/CVD were registered. The Iraqi Cardio-Oncology Program-Pharmacist (ICOP-Pharm) paradigm was designed to involve a qualified cardiology pharmacist for initial cardiovascular (CV) drug interventions.

Results

Among 333 patients who attended our clinic over the 1-year interval, 200 (60%) CVRF/CVD cases were enrolled in the study, and of them 79 (40%) patients had CV drug interventions. A total of 196 interventions were done, including 147 (75%) cases performed by the cardiology pharmacist, and 92 (63%) of the latter were CV drug initiations. Among the total CVRF/CVD treated initially by the cardiology pharmacist, hypertension 32 (26%) and cancer therapy-related cardiac dysfunction 29 (24%) were the main types.

Conclusion

The qualified cardiology pharmacist was responsible for three-quarters of the initial CV drug interventions at the cardio-oncology clinic in a complementary approach to the cardiologist. The role of the cardiology pharmacist in the ICOP-Pharm paradigm may be one of the reasons for the ability of the heart team to manage 3-fold of the patient volume when compared with those in the United Kingdom or Canada.
Mechanism-based targeting of cardiac arrhythmias by phytochemicals and medicinal herbs: A comprehensive review of preclinical and clinical evidence
Authors: Soltani Danesh , Azizi Bayan , Rahimi Roja , Talasaz Azita H. , Rezaeizadeh Hossein , Vasheghani-Farahani Ali
Published: September 2022

Cardiac arrhythmias, characterized by an irregular heartbeat, are associated with high mortality and morbidity. Because of the narrow therapeutic window of antiarrhythmic drugs (AADs), the management of arrhythmia is still challenging. Therefore, searching for new safe, and effective therapeutic options is unavoidable. In this study, the antiarrhythmic effects of medicinal plants and their active constituents were systematically reviewed to introduce some possible candidates for mechanism-based targeting of cardiac arrhythmias. PubMed, Embase, and Cochrane library were searched from inception to June 2021 to find the plant extracts, phytochemicals, and multi-component herbal preparations with antiarrhythmic activities. From 7337 identified results, 57 original studies consisting of 49 preclinical and eight clinical studies were finally included. Three plant extracts, eight multi-component herbal preparations, and 26 phytochemicals were found to have antiarrhythmic effects mostly mediated by affecting K+channels, followed by modulating Ca2+channels, upstream target pathways, Navchannels, gap junction channels, and autonomic receptors. The most investigated medicinal plants wereRhodiola crenulataandVitis vinifera.Resveratrol, Oxymatrine, and Curcumin were the most studied phytochemicals found to have multiple mechanisms of antiarrhythmic action. This review emphasized the importance of research on the cardioprotective effect of medicinal plants and their bioactive compounds to guide the future development of new AADs. The most prevalent limitation of the studies was their unqualified methodology. Thus, future well-designed experimental and clinical studies are necessary to provide more reliable evidence.
Design a regional and multistep air quality forecast model based on deep learning and domain knowledge
Authors: Mo Xinyue , Li Huan , Zhang Lei
Published: September 2022

Air pollution is an issue across the world. It not only directly affects the environment and human health, but also influences the regional and even global climate by changing the atmospheric radiation budget, resulting in extensive and serious adverse effects. It is of great significance to accurately predict the concentration of pollutant. In this study, the domain knowledge of Atmospheric Sciences, advanced deep learning methods and big data are skillfully combined to establish a novel integrated model TSTM, derived from its fundamental features of Time, Space, Type and Meteorology, to achieve regional and multistep air quality forecast. Firstly, Expectation Maximization and Min-Max algorithms are used for the interpolation and normalization of data. Secondly, feature selection and construction are accomplished based on domain knowledge and correlation coefficient, and then Sliding Time Window algorithm is employed to build the supervised learning task. Thirdly, the features of pollution source and meteorological condition are learned and predicted by CNN-BiLSTM-Attention model, the integrated model of convolutional neural network and Bidirectional long short-term memory network based on Sequence to Sequence framework with Attention mechanism, and then Convolutional Long Short-Term Memory Neural Network (ConvLSTM) integrates the two determinant features to obtain predicted pollutant concentration. The multiple-output strategy is also employed for the multistep prediction. Lastly, the forecast performance of TSTM for pollutant concentration, air quality and heavy pollution weather is tested systematically. Experiments are conducted in Beijing-Tianjin-Hebei Air Pollution Transmission Channel (“2+26” cities) of China for multistep prediction of hourly concentration of six conventional air pollutants. The results show that the performance of TSTM is better than other benchmark models especially for heavy pollution weather and it has good robustness and generalization ability.
Augmented reality applications as a digital learning innovation in response to the pandemic
Authors: Batool Hira
Published: September 2022

Augmented reality applications are digital learning innovations that can rapidly improve the learning of college and university students in the virtual setting. Ever since the beginning of the pandemic, on-site teaching has been pushed back to limit the spread of disease. This paper examines the attitudes and subjective and behavioral norms of 100 college students in Thailand toward digital learning innovations accessed through augmented reality applications. The study offers a theoretical model established through the theory of planned behavior (TPB) and uses it to investigate students’ choices of augmented reality applications as digital learning innovations in their courses. The data were analyzed through structural equation modeling. The findings showed that students’ acceptance of digital learning innovations through augmented reality applications could be explained through TPB wherever attitudinal and subjective factors showed significant influence; meanwhile, perceived behavioral control did not demonstrate a significant influence on college students. The findings acknowledge the positive effects of augmented reality applications as experienced by college students in Thailand.
Hypertensive disorders during pregnancy and polycystic ovary syndrome are associated with child communication and social skills in a sex-specific and androgen-dependent manner
Authors: Firestein Morgan R. , Romeo Russell D. , Winstead Hailey , Goldman Danielle A. , Grobman William A. , Haas David M. , Parry Samuel , Reddy Uma M. , Silver Robert M. , Wapner Ronald J. , Champagne Frances A.
Published: September 2022

Prenatal exposure to testosterone is implicated in the etiology of autism spectrum disorder (ASD). Hypertensive disorders of pregnancy and polycystic ovary syndrome are associated with both hyperandrogenism and increased risk for ASD. We examined whether increased maternal testosterone mediates the relationship between these hyperandrogenic disorders (HDs) during pregnancy and child communication and social skills. Maternal plasma was collected during the second trimester and parent-report measures of child communication and social skills were obtained at 4.5-6.5 years of age from 270 participants enrolled in the Nulliparous Pregnancy Outcomes Study: Monitoring Mothers-to-be (nuMoM2b). Our retrospective frequency-matched cohort study design identified 58 mothers with one or both of the HDs and 58 matched controls. Women diagnosed with an HD who carried a female had higher testosterone levels compared to those carrying a male (t(56) = -2.70, p = 0.01). Compared to females controls, females born to women with an HD had significantly higher scores on the Social Communication Questionnaire (t(114) = -2.82, p =0.01). Maternal testosterone partially mediated the relationship between a diagnosis of an HD and SCQ scores among females. These findings point to sex-specific associations of two HDs – hypertensive disorders of pregnancy and polycystic ovary syndrome – on child communication and social skills and a mediating effect of maternal testosterone during pregnancy. Further research is needed to understand placental-mediated effects of maternal testosterone on child brain development and neurodevelopmental outcomes.
Exenatide improves hepatocyte insulin resistance induced by different regional adipose tissue
Authors: Bai Chuanmin , Wang Yujun , Niu Zhi , Guan Yaxin , Huang Jingshan , Nian Xin , Zuo Fan , Zhao Juan , Kazumi Tsutomu , Wu Bin
Published: September 2022

Obesity is resulted from energy surplus and is characterized by abnormal adipose tissue accumulation and/or distribution. Adipokines secreted by different regional adipose tissue can induce changes in key proteins of the insulin signaling pathway in hepatocytes and result in impaired hepatic glucose metabolism. This study aimed to investigate whether exenatide affects key proteins of IRS2/PI3K/Akt2 signaling pathway in hepatocytes altered by the different regional fat depots. Six non-obese patients without endocrine diseases were selected as the research subjects. Their subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT)were co-cultured with HepG2 cells in the transwell chamber. In the presence or absence of exenatide, adipokines content in the supernatant of each experimental group was detected by ELISA. In addition, HepG2 cells in each co-culture group with and without insulin were collected, and the expression of key proteins IRS2, p-IRS2(S731), PI3K-p85, Akt2, and p-Akt2(S473) was detected by western blotting (WB). The results showed that the adipokines IL-8, MCP-1, VEGF, and sTNFR2 in the supernatant of HepG2 cells induced by different regional adipose tissue were significantly higher than those in the HepG2 group, and VAT released more adipokines than SAT. Furthermore, these adipokines were significantly inhibited by exenatide. Importantly, the different regional fat depot affects the IRS2/PI3K/Akt2 insulin signaling pathway of hepatocytes. Exenatide can up-regulate the expression of hepatocyte proteins IRS2, PI3K-p85, p-Akt2(S731) inhibited by adipose tissue, and down-regulate the expression of hepatocyte proteins p-IRS2(S731) promoted by adipose tissue. The effect of VAT on the expression of these key proteins in hepatocytes is more significant than that of SAT. But there was no statistical difference in the expression of Akt2 protein among each experimental group, suggesting that exenatide has no influence on the expression of Akt2 protein in hepatocytes. In conclusion, exenatide may improve hepatic insulin resistance (IR) by inhibiting adipokines and regulating the expression of key proteins in the IRS2/PI3K/Akt2 pathway.
AST-to-ALT ratio in the first trimester and the risk of gestational diabetes mellitus
Authors: An Rongjing , Ma Shujuan , Zhang Na , Lin Huijun , Xiang Tianyu , Chen Mengshi , Tan Hongzhuan
Published: September 2022

Background

Aspartate aminotransferase-to-alanine transaminase ratio (AST/ALT) has been reported affect the risk of type 2 diabetes (T2DM), but it is uncertain if it has relationship with gestational diabetes mellitus (GDM).

Objectives

Our study aimed to investigate the association between AST/ALT ratio in the first trimester and the risk of subsequent development of GDM.

Method

This prospective cohort study enrolling 870 pregnant women, 204 pregnant women with missing data or liver diseases were excluded, 666 pregnant women were included in this study containing 94 GDM women. Blood samples were collected in the first trimester. Univariate analysis and multivariate logistic regression were used to evaluate the association between AST/ALT and GDM. Nomogram was established based on the results of multivariate logistic analysis. Receiver Operating Characteristic (ROC) curves and calibration curves were used to evaluate the predictive ability of this nomogram model for GDM. Decision curve analysis (DCA) was used to examine the clinical net benefit of predictive model.

Results

AST/ALT ratio (RR:0.228; 95% CI:0.107-0.488) was associated with lower risk of GDM after adjusting for confounding factors. Indicators used in nomogram including AST/ALT, maternal age, preBMI, waist circumference, glucose, triglycerides, high density lipoprotein cholesterol and parity. The area under the ROC curve (AUC) value of this predictive model was 0.778, 95% CI (0.724, 0.832). Calibration curves for GDM probabilities showed acceptable agreement between nomogram predictions and observations. The DCA curve demonstrated a good positive net benefit in the predictive model.

Conclusions

The early AST/ALT level of pregnant women negatively correlated with the risk of GDM. The nomogram including AST/ALT at early pregnancy shows good predictive ability for the occurrence of GDM.
Prognostic factors and mitotane treatment of adrenocortical cancer. Two decades of experience from an institutional case series
Authors: Tőke Judit , Uhlyarik Andrea , Lohinszky Júlia , Stark Júlia , Huszty Gergely , Micsik Tamás , Borka Katalin , Reismann Péter , Horányi János , Igaz Peter , Tóth Miklós
Published: September 2022

Objectives

This study aimed to characterise the clinicopathological features and prognostic factors of a large cohort of Hungarian patients with adrenocortical cancer diagnosed between 2000-2021.

Patients and methods

This retrospective study included seventy-four patients (27 men and 47 women) with histologically confirmed adrenocortical cancer in a single tertiary referral endocrine centre. Descriptive statistics were performed, providing summaries of selected clinical and pathological parameters. Clinicopathological factors contributing to overall survival were analysed.

Results

The median age of patients was 48,5 years (17-84 years) at diagnosis. The majority of cases were diagnosed at ENSAT stage II (39,2%) and stage IV (33,8%). At diagnosis, the median tumour size was 9,0 cm (4,5-20 cm). In 47 patients (71,6%), the tumour was hormonally active. The median overall survival and the 5-year survival rate were 23,5 months (95% CI, 17-30,5 months) and 18,3%, respectively. Primary tumour resection was performed in 68 patients (91,8%); R0 surgical resection was achieved in 30 patients. In univariate Cox regression model, tumours with stages III and IV, high proliferative activity (Ki67-index > 10%), R1-R2 surgical resection state and hormonal activity were associated with poorer survival. Cortisol excess, both isolated and combined with androgen production, was associated with poorer survival. Fifty-five patients were treated with mitotane. The overall survival of patients achieving therapeutic mitotane plasma concentration was significantly better compared to those who never reached it [27.0 (2-175) months vs 18.0 (2-83) months; p<0.05)]. The median age, the distribution of gender, ENSAT stage, resection state and Ki67-index did not differ between these two groups. The time needed to reach the therapeutic range of serum mitotane was 96.5 days (95% CI, 75-133 days).

Conclusion

Our results confirm previous data that disease stage, mitotic activity, the resection state and the mitotane treatment achieving therapeutic concentration are the most critical parameters influencing the prognosis of adrenocortical cancer. Our data suggest that hormonal activity may be more frequent than described previously, and it is a strong and independent prognostic factor of overall survival. To our knowledge, this is the first single-centre study confirming the prognostic importance of achieving therapeutic mitotane concentration.
Primary aldosteronism and obstructive sleep apnea: What do we know thus far?
Authors: Loh Huai Heng , Sukor Norlela
Published: September 2022

Both primary aldosteronism and obstructive sleep apnea are well-known causes of hypertension and contribute to increased cardiovascular morbidity and mortality independently. However, the relationship between these two entities remains unclear, with studies demonstrating contradictory results. This review aims to collate and put into perspective current available research regarding the association between primary aldosteronism and obstructive sleep apnea. The relationship between these two entities, clinical characteristics, clinical implications, outcomes of treatment, potential causal links and mechanisms are hereby presented.
MiR-539-3p impairs osteogenesis by suppressing Wnt interaction with LRP-6 co-receptor and subsequent inhibition of Akap-3 signaling pathway
Authors: Tripathi Alok , John Aijaz A. , Kumar Deepak , Kaushal Saurabh Kumar , Singh Devendra Pratap , Husain Nazim , Sarkar Jayanta , Singh Divya
Published: September 2022

X-linked hypophosphatemia (XLH), an inheritable form of rickets is caused due to mutation in Phex gene. Several factors are linked to the disease’s aetiology, including non-coding RNA molecules (miRNAs), which are key post-transcriptional regulators of gene expression and play a significant role in osteoblast functions. MicroRNAs sequence analysis showed differentially regulated miRNAs in phex silenced osteoblast cells. In this article, we report miR-539-3p, an unidentified novel miRNA, in the functional regulation of osteoblast. MiR-539-3p overexpression impaired osteoblast differentiation. Target prediction algorithm and experimental confirmation by luciferase 3’ UTR reporter assay identified LRP-6 as a direct target of miR-539-3p. Over expression of miR-539-3p in osteoblasts down regulated Wnt/beta catenin signaling components and deteriorated trabecular microarchitecture leading to decreased bone formation in ovariectomized (Ovx) mice. Additionally, biochemical bone resorption markers like CTx and Trap-5b were elevated in serum samples of mimic treated group, while, reverse effect was observed in anti-miR treated animals along with increased bone formation marker P1NP. Moreover, transcriptome analysis with miR-539-3p identified a novel uncharacterized Akap-3 gene in osteoblast cells, knock down of which resulted in downregulation of osteoblast differentiation markers at both transcriptional and translational level. Overall, our study for the first time reported the role of miR-539-3p in osteoblast functions and its downstream Akap-3 signalling in regulation of osteoblastogenesis.
The serum free triiodothyronine to free thyroxine ratio as a potential prognostic biomarker of chronic kidney disease in patients with glomerular crescents: A retrospective study
Authors: Zhang Liwen , Wu Yuxiao , Nie Yuxin , Lv Wenlv , Li Yang , Zhu Bowen , Jin Shi , Shen Ziyan , Li Fang , Liu Hong , Fang Yi , Ding Xiaoqiang
Published: September 2022

Background

Crescent formation indicates severe glomerular pathology, and hypothyroidism usually predicts poor prognosis for severe diseases. However, the relationship between thyroid function and the progression of chronic kidney disease (CKD) is unclear. This study analysed the prognostic predictive value of the serum free triiodothyronine (FT3) to free thyroxine (FT4) ratio and its correlation with renal function in patients with CKD with crescent formation.

Methods

This single-centre study included 162 CKD patients with glomerular crescents confirmed by renal pathology between March 2012 and December 2014. According to the first tertile (0.284) of FT3/FT4 ratio, the patients were divided into high and low FT3/FT4 ratio groups. Kaplan-Meier and Cox regression analyses were performed to evaluate the prognostic value of the FT3/FT4 ratio.

Results

The age, haemoglobin, eGFR, urinary albumin-to-creatinine ratio, cardiac troponin T, N-terminal brain natriuretic peptide precursor, FT3, FT4, percentage of total crescents in non-globally sclerotic glomeruli, prevalences of hypertension, moderate to severe renal tubulopathy and crescentic nephritis, and proportion of patients receiving glucocorticoids and immunosuppressants were significantly different between high and low FT3/FT4 ratio groups (P < 0.05). Multivariate Cox regression analysis showed that when compared with patients with a high FT3/FT4 ratio (>0.284), those with intermediate and low FT3/FT4 ratios (≤0.284) had an increased risk of the long-term composite endpoint (P < 0.05 for various adjustment models).

Conclusions

A low FT3/FT4 ratio is associated with increased mortality and worse outcome risk in CKD patients with crescent pathology.