bluuebunny/crossref_metadata_2025 huggingface dataset:
Recomendações para a proteção da privacidade do paciente
Authors: Carvalhal Gustavo Franco , Poli Marcelino Hofmeister , Clementel Fabiano Kingeski , Gauer Gabriel Chittó , Marques Graziela Hax , Silveira Inês G. , Piccoli João Manuel , Pithan Lívia Haygert , Guilhermano Luiz Gustavo , Ferreira Magda , Bonhemberger Marcelo , Figueiredo Maria Antonia Zancanaro de , Gil Maria Estelita , Badalotti Mariangela , Debiasi Marcio , Souza Paulo Vinícius Sporleder de , Kik Raquel Milani El , Petersen Vera Maria , Kipper Délio José
Published: April 2017

Resumo A preocupação sobre aspectos bioéticos da privacidade do indivíduo e da privacidade dos dados de seus atendimentos é crescente no meio médico. Processos propedêuticos e terapêuticos atuais requerem envolvimento multidisciplinar de uma série de indivíduos, especialmente em se tratando de internações hospitalares. A transmissão e o armazenamento das informações clínicas e laboratoriais dos pacientes envolvem diferentes mídias, com problemas inerentes de acessibilidade e proteção da informação. Os autores sugerem situações hipotéticas que exemplificam problemas comumente abordados na atuação de comitê de bioética clínica, contextualizando-os no Brasil e no mundo, e sugerindo passos para minimizar potenciais problemas de quebra de privacidade e confidencialidade.
Bioética, pesquisa qualitativa e equilíbrio reflexivo
Authors: Ribeiro Carlos Dimas Martins
Published: April 2017

Resumo Trata-se de estudo de natureza teórica, em que se pretende discutir o uso do equilíbrio reflexivo no processo de análise de dados em pesquisa qualitativa em bioética. Na primeira parte é discutido o papel da pesquisa qualitativa em bioética. Na segunda parte é analisado o uso desse procedimento nesses estudos. O equilíbrio reflexivo é uma forma de justificação moral que objetiva o ajustamento mútuo entre a teoria ética adotada pela pesquisa, os julgamentos morais considerados pelo(s) pesquisador(es) e a experiência moral dos participantes da pesquisa, pressupondo certos fatos moralmente relevantes, de forma a tecer rede de crenças coerente entre esses componentes. Nesse processo, tanto a teoria ética como a experiência moral podem ser passíveis de crítica.
Comitês de ética em pesquisa no Brasil: estudo com coordenadores
Authors: Jácome Marília de Queiroz Dias , Araujo Tereza Cristina Cavalcanti Ferreira de , Garrafa Volnei
Published: April 2017

Resumo Este estudo buscou caracterizar perfil e operacionalização de Comitês de Ética em Pesquisa. Foi enviado questionário eletrônico aos 645 comitês existentes à época e respondido por 129 coordenadores. As respostas foram categorizadas por frequência e média e passaram por teste estatístico. Os resultados obtidos indicaram que a maioria dos coordenadores concluíra mestrado ou doutorado em Ciências Biológicas e da Saúde. Os comitês funcionavam há mais de nove anos em instituições de ensino superior com apoio institucional insuficiente. Os membros eram capacitados por meio da leitura das regulamentações e orientações do Comitê. A distribuição de protocolos era feita por afinidade temática, e a decisão grupal se dava por consenso ou votação. Conclui-se que os comitês estão consolidados e cumprem a regulamentação ética, mas necessitam dialogar mais com pesquisadores e a Comissão Nacional de Ética em Pesquisa.
Vulnerabilidade de adolescentes em pesquisa e prática clínica
Authors: Santos Débora de Oliveira , Gomes Fabíola Alves , Teixeira Kely Raspante , Roever Leonardo , Fuzissaki Marceila de Andrade , Faleiros Tales , Lima Taís de Campos
Published: April 2017

Resumo Os adolescentes são considerados grupo vulnerável e exposto a diferentes ameaças à saúde, tornando-se necessária a discussão sobre aspectos éticos relacionados a sua participação em pesquisa e prática clínica. Por meio de revisão integrativa de literatura foram selecionados estudos que abordaram aspectos bioéticos relacionados à vulnerabilidade de adolescentes nos últimos quinze anos. Nove artigos atenderam aos critérios pré-estabelecidos para o estudo e foram agrupados em três categorias: 1) trabalhos que pontuaram a compreensão psicológica e cognitiva do adolescente ao participar de pesquisa científica; 2) estudos que enfatizaram aspectos relacionados a decisões médicas; e 3) estudos que abordaram a temática da sexualidade na adolescência. Após análise dos estudos selecionados, verificou-se que não é possível chegar a consenso válido para todas as situações que envolvem adolescentes em pesquisa e prática clínica.
Transplante renal em Alagoas: olhar bioético sobre a vulnerabilidade de quem precisa
Authors: Santos Carlos Adriano Silva dos , Hossne William Saad , Anjos Marcio Fabri dos
Published: April 2017

Resumo Apesar da necessidade crescente, a quantidade de transplantes renais realizados no estado de Alagoas é muito baixa. O objetivo do estudo foi analisar se os gestores contribuíram para o aumento da vulnerabilidade de pacientes que necessitam de transplante renal no estado. A amostra foi composta por gestores da Secretaria Estadual de Saúde; coordenadores da organização de procura de órgãos e tecidos; equipes de transplante; médicos de clínicas de nefrologia; médicos dos serviços de atendimento móvel de urgência; médicos das unidades de terapia intensiva; e o representante da Associação dos Renais Crônicos e Transplantados de Alagoas. Resultou em 14 participantes que apontaram falhas gerenciais nos programas e recursos voltados ao transplante. Concluiu-se que necessitados de transplante renal em Alagoas são mais vulneráveis devido a falhas na gestão pública de saúde.
Conflitos bioéticos: atendimento fisioterapêutico domiciliar a pacientes em condição de terminalidade
Authors: Silva Lízia Fabíola Almeida , Lima Maria da Glória , Seidl Eliane Maria Fleury
Published: April 2017

Resumo O debate bioético enseja reflexões que propiciam a compreensão da morte e da terminalidade de modo a assegurar a observância de princípios como respeito à autonomia, beneficência e não maleficência e os direitos humanos. O objetivo do estudo foi analisar conflitos bioéticos no trabalho de fisioterapeutas em atendimento domiciliar a pacientes em condição de terminalidade. Trata-se de estudo qualitativo e descritivo. Dez fisioterapeutas do Distrito Federal participaram, respondendo a entrevista semiestruturada. Duas categorias foram identificadas: “desafios da atuação em domicílio com pacientes em condição de terminalidade”; e “o fisioterapeuta entre o tecnicismo e o humanismo”. O estudo descortina conflitos bioéticos potenciais no atendimento a esses pacientes e seus familiares, em que os limites para a utilização dos recursos terapêuticos se traduzem em posturas polarizadas – de aproximação ou distanciamento – e o desafio de promover cuidado pautado na humanização e na dignidade humana.
Uma perspectiva da Clínica Ampliada: as práticas da Psicologia na Assistência Social
Authors: Dettmann Ana Paula da Silva , Aragão Elizabeth Maria Andrade , Margotto Lilian Rose
Published: April 2017

Resumo O cenário contemporâneo da Política Pública de Assistência Social é apresentado através da Psicologia Institucional, que direciona a uma certa visão da Psicologia sobre a Política de Assistência Social. O objetivo da presente pesquisa foi investigar a visão dos psicólogos sobre essa política e as práticas que eles próprios desenvolvem. Os depoimentos de onze psicólogos atuantes na Política e no Fórum de Trabalhadores do SUAS, investigados pela História Oral, apresentaram as práticas da Psicologia na Assistência Social, tendo sido destacada a questão do poder entremeado às estratégias biopolíticas de controle e resistências, que ora reproduzem normatizações, ora produzem linhas de fugas. Ainda, ressaltou a perspectiva da Clínica Ampliada com as possibilidades de transformação dos sujeitos e da sociedade, a partir de uma visão clínica e crítica da Psicologia e da Política de Assistência Social.
Yield and nutritive value of the silage of corn intercropped with tropical perennial grasses
Authors: Costa Nídia Raquel , Andreotti Marcelo , Crusciol Carlos Alexandre Costa , Lima César Gustavo da Rocha , Castilhos André Michel de , Souza Daniel Martins de , Bonini Carolina dos Santos Batista , Pariz Cristiano Magalhães
Published: April 2017

Abstract: The objective of this work was to compare the yield and nutritive value of the silage of corn intercropped with palisade grass (Urochloa brizantha 'Marandu') or guinea grass (Megathyrsus maximus 'Tanzânia') with those of the silage of monocropped corn, as well as to evaluate the pasture established in the intercropping systems during two growing seasons (2010-2011 and 2011-2012), in low-altitude Brazilian Cerrado. The treatments consisted of three cropping systems: monocropped corn, corn intercropped with palisade grass, and corn intercropped with guinea grass, with four replicates. Intercropping decreased corn forage dry matter yield for silage; however, due to the addition of grass, total dry matter yield (corn + grass) was similar between treatments. Intercropping also did not negatively affect corn production components and morphological characteristics. The cropping systems provided silages with good nutritive values, and the inclusion of tropical forages increased the silage fiber contents. Intercropping corn with tropical perennial grasses is a viable option for producing large quantities of silage with good nutritive value. The forage yield (silage + pasture) of these intercropping systems is similar to that of monocropped corn in tropical regions and has the advantage of providing a pasture in the off-season.
Agronomic characterization of high-yielding irrigated barley accessions in the Cerrado
Authors: Sayd Ricardo Meneses , Amabile Renato Fernando , Faleiro Fábio Gelape , Montalvão Ana Paula Leite , Coelho Mateus Costa
Published: April 2017

Abstract: The objective of this work was to estimate the genetic, phenotypic, and environmental parameters of 113 barley accessions (Hordeum vulgare), previously selected based on high yield, in an irrigated production system in the Cerrado (Brazilian savanna), for use in breeding programs in Brazil. The experiment was conducted in 2013 at two sites in the Federal District, using a randomized complete block design with three replicates. The evaluated traits were: grain yield, kernel plumpness, thousand grain weight, plant height, lodging, and days to heading. Significant differences were observed between environment and accessions for all studied traits, as well as genotype x environment interaction. The MCU 3832 PI 402310, MCU 3484 PI 401962, CI 08053 Custer, MCU 3634 PI 402112, and MCU 3816 PI 402294 accessions stood out regarding grain yield and kernel plumpness. It is possible to obtain high selection gains with high heritability values. However, indirect selection is not recommended in the experimental conditions due to the low genotypic and phenotypic correlations obtained. It is also possible to select barley accessions with high yield and early heading date simultaneously, an important factor in the genotype selection process for future experiments. The accessions from Colombia are the most adapted to the Cerrado conditions.
Extended storage of cold raw milk on yogurt manufacturing
Authors: Fagnani Rafael , Schuck Josiane , Botaro Bruno Garcia , Santos Fernando Cesar dos
Published: April 2017

Abstract: The objective of this work was to evaluate the effect of the extended cold storage of raw milk on the kinetics of fermentation on yogurt production, as well as on the product’s microbiological and physicochemical properties during shelf life. Three treatments were evaluated: yogurts made with raw milk stored for 4, 72, and 168 hours. Kinetics of fermentation was assessed through the lactic acid production rate, growth rate of Streptococcus thermophilus and Lactobacillus bulgaricus, and time to reach pH 5.0 and 4.5. The physicochemical and microbiological characteristics of raw milk and yogurts were also analyzed during cold storage at 4°C. The microbial quality of raw milk was affected by the storage time of 168 hours, with a decreasing tendency in the mesophiles:psychrotrophs ratio. Extended storage of raw milk beyond 72 hours negatively affects yogurt production, despite the low initial bacterial count, decreasing lactic acid production, S. thermophilus growth rate, pH, and protein content. Therefore, to optimize fermentation and yogurt shelf life, the maximum storage time for raw milk at 4oC should not exceed 72 hours.
FLUAZIFOP-P-BUTYL EFFICACY AS A FUNCTION OF APPLICATION TIME AND HERBICIDE DOSE
Authors: CIESLIK L.F. , VIDAL R.A. , MACHADO A.B. , TREZZI M.M.
Published: April 2017

ABSTRACT Grass weeds are common in summer crops and strongly decreases the grain yield of the common bean crop. The time of herbicide application influences the variability of environmental conditions and affects the product performance. The objectives of this work were to identify the time of fluazifop-p-butyl (fluazifop) application which gives best grass weed control in the common bean crop and to elucidate the environmental variables most important for the efficacy of this herbicide. Field experiments were conducted involving five application times (2 a.m., 6 a.m., 11 a.m., 4 p.m. and 9 p.m.) and five doses of fluazifop (80, 110, 140, 170 and 200 g ha-1), with additional no-herbicide control. At the time of the herbicide application it was determined the air temperature, relative humidity, the photosynthetically active radiation (PAR) and the leaf angle, whereas the weed control and the dry mass of the weed Urochloa plantaginea was assessed at 20 days after treatment (DAT). Efficacy on grass control with fluazifop was dependent on the herbicide dose and on the time of day that the product was applied. Spray at early morning hours (6 a.m.) showed better efficacy on weed control in relation to periods during warmer conditions of the day (11 a.m. and 4 p.m.). Nocturnal fluazifop application had better weed control when compared to herbicide sprayed in the afternoon. The air temperature, relative humidity and PAR were correlated to weed leaf angle, which correlated the most with fluazifop performance.
SENSITIVITY OF MORNINGGLORY PLANTS AND THEIR PROGENIES TO GLUFOSINATE AMMONIUM
Authors: BRITO I.P.F.S. , MORAES C.P. , MARCHESI B.B. , CARBONARI C.A. , VELINI E.D.
Published: April 2017

ABSTRACT The sensitivity of weed species to herbicides is variable, and the behavior can be repeated or not in the next generation. The objective of this study was to evaluate the response of morningglory plants to different doses of glufosinate ammonium and the sensitivity of plant populations and their progenies to the herbicide. Three studies were conducted, all in a greenhouse and repeated in two periods. In the first study, two experiments were conducted to examine the dose-response curve, and the treatments were seven different doses of the herbicide glufosinate ammonium (0, 50, 100, 200, 400, 800, and 1,600 g a.i. ha-1), with four replications each. In the second study, which examined the range in sensitivity of morningglory to glufosinate ammonium, 44 plants were sprayed with a dose of 200 g a.i. ha-1 of the herbicide. Finally, in the third study, the range in sensitivity of morningglory progenies to glufosinate ammonium was investigated; in this experiment, the progenies of seven of the previous plants were sprayed with 200 g a.i. ha-1 of herbicide. The ammonium contents in the tissues were measured, and percent injury were visually assessed. Ammonium content in morningglory leaves was increased more than seven times by glufosinate application and the maximum ammonium content was observed for the highest herbicide dose. Variability existed in the ammonium content among the individuals of the morningglory population; however, the behavior was not replicated in the same way in progenies. Plants survival after herbicide application allows the production of progenies with wide variability in their sensitivity to the product, regardless of the behavior verified in progenitor plants.
Crisis económica y profesionalización en el campo de la educación ambiental: comparativa 2007-2013 en Galicia
Authors: Cartea Pablo Ángel Meira , Núñez María Barba , Castiñeiras Juan José Lorenzo
Published: April 2017

Resumen El objetivo del artículo es aportar evidencias que permitan valorar cómo está incidiendo en el campo de la educación ambiental (en adelante EA) la crisis social y económica que padece la sociedad española. Para ello se analiza, a través de una encuesta online, una muestra de profesionales que trabajan en este campo en Galicia, utilizando como principal perspectiva analítica la comparación de su situación socio-laboral en el albor de la crisis, año 2007, con la experimentada a principios de 2013. Pese a centrarse territorialmente en Galicia, el análisis apunta interpretaciones de la realidad social que pueden ser extrapolables a otros contextos, proponiendo una lectura compleja que inscribe el desmantelamiento del campo de la EA dentro de la degradación de un estado del bienestar que era incipiente, con el debilitamiento sistemático de los servicios y recursos públicos que trabajan por el bien común. Los datos evidencian este desmantelamiento, así como ciertas debilidades internas del campo de la EA que lo hacen especialmente vulnerable. Ante esta situación, se defiende la necesidad de una política pública de EA, que debe tener en la Administración Autonómica su principal actor responsable, que contribuya a consolidar y estabilizar el campo. El propio campo se enfrenta al reto de conquistar un papel protagonista en la construcción participada de dichas políticas.
Isócrates, professor de philosophía
Authors: Pagotto-Euzebio Marcos Sidnei
Published: April 2017

Resumo O artigo apresenta o magistério de Isócrates (436-338 a.C.), autor ateniense contemporâneo de Platão, e suas concepções acerca da forma e dos propósitos da paideía ou educação, que ele denominava, em seu conjunto, philosophía. Para tanto, descrevem-se o rol dos alunos que Isócrates teria tido, a popularidade de sua escola e o testemunho, por outros autores da Antiguidade, de sua influência educativa. Em seguida, discute-se a definição isocrática de philosophía, apresentada, por vezes, como um empenho intelectual conjugado à experiência; em outros momentos, como a cultura ou paideía criada por Atenas, cidade em que o lógos, em seu duplo sentido de razão e discurso, ocupava lugar de excepcional importância. Por fim, passa-se dessa definição geral de philosophía para aquela que a apresenta como um procedimento educativo deliberado, sendo que o professado por Isócrates em sua escola seria, segundo ele, o único a merecer, de todo direito, o título de philosophía. Descreve-se, então, a philosophía de Isócrates, com seus princípios mais importantes, a dóxa, ou opinião; a empeiría, ou experiência, e o kairós, ou ocasião, indicando as justificativas para essa opção e os resultados esperados de tal programa educativo, e de que modo tais princípios estariam presentes no que chamaríamos de seus métodos pedagógicos, dos quais se destaca a prática de Isócrates de submeter suas próprias obras à crítica de seus alunos, em um formato de discussão ou debate próximo do que denominaríamos, hoje, de seminário.
Adherence and factors related to acceptance of alcohol for antiseptic hand rubbing among nursing professionals
Authors: Oliveira Adriana Cristina de , Gama Camila Sarmento , Paula Adriana Oliveira de
Published: April 2017

Abstract OBJECTIVE Identify rates of adhesion and related factors to acceptance of an alcohol based preparation to hands antiseptic friction among nursing professionals in a unit of intensive therapy. METHOD A cross-sectional study, which involved direct observation of hand hygiene opportunities and nursing professionals’ completion of questionnaires, was conducted at a university hospital between January and July 2015. Descriptive and univariate analyses were performed, with a 5% significance level. RESULTS It was observed 956 opportunities of hand hygiene among 46 nursing professionals. The rate of adhesion to alcohol-based handrub (ABH) was 34.8% and about 87.0% preferred handwashing. Nurses used ABH more frequently than nursing technicians (p <0.001), and the report of feeling of clean hands after using the alcohol product was directly related to higher rates of adherence to antiseptic friction through observation (P <0.05). CONCLUSION The finding indicating low ABH usage highlights the need for greater institutional investment in strategies that help health professionals to recognize the advantages of this type of HH with respect to time spent, ease of access to dispensers, effectiveness in eliminating microorganisms, and maintaining skin moisturization.
Controlling Human Papilloma Virus: A Public Health Perspective of Treatment of Anogenital Warts
Authors: Tanveer Nadeem
Published: April 2017

The success of human papilloma virus (HPV) screening is attributable to clear-cut guidelines on the detection and management of premalignant HPV lesions. A similar approach is necessary for both low-risk and high-risk lesions, including anogenital warts.
Die hedendaagse Afrikaanse poësiesisteem: Op soek na die mees verteenwoordigende wiskundige model van die rolspelerverhoudings daarbinne
Authors: Senekal B
Published: April 2017

Die sisteemteoretiese benadering tot die literatuurstudie is reeds deeglik gevestig, ook in Afrikaans. Sisteme kan as netwerke gemodelleer word en sedert die vyftigerjare van die vorige eeu is 'n groot aantal netwerkmodelle binne die wiskundige grafiekteorie ontwikkel. Die huidige studie vergelyk die hedendaagse Afrikaanse poësiesisteem en die Afrikaanse literêre sisteem van ander tydperke met twee netwerkmodelle ten einde te bepaal watter netwerkmodel die juisste voorstelling van Afrikaanse literêre netwerke verteenwoordig. Twee van die belangrikste netwerkmodelle in die wiskundige grafiekteorie, naamlik die lukrake netwerkmodel van Erdös en Rényi (1959; 1960 - voortaan die ER-model) en die skaalvrye netwerkmodel van Barabási en Albert (1999 - voortaan die BA-model), word bespreek en met behulp van Pearson se korrelasiekoëffisiënt word die korrelasies tussen die skakelverspreidingspatrone van Afrikaanse literêre netwerke en hierdie twee netwerkmodelle bereken. Daar word aangetoon dat sowel die hedendaagse Afrikaanse poësiesisteem as ander Afrikaanse literêre netwerke akkuraat met die BA-model gemodelleer kan word. Die betekenis van hierdie modellering word bespreek, veral met betrekking tot hoe die groei van die literêre netwerk daar uitsien, dit wil sê, deur Barabási se beginsel van selektiewe skakelvorming op die hedendaagse Afrikaanse poësie van toepassing te maak.
Leerders se ervarings tydens blogging in 'n Afrikaansklas
Authors: Mihai M
Published: April 2017

Hierdie studie het op blogging gefokus, as een van die moontlike maniere waarop 'n rekenaar in 'n Afrikaansklas gebruik kan word, met die doel om integrasie tussen die verskillende leerareas te bevorder. Die navorser wou bepaal hoe leerders blogging sou hanteer, met die fokus op die verskillende komponente in die skoolomgewing - die gebruik van tegnologie, die leerders self, die reëls waaraan hulle moes voldoen en die gemeenskap waarin hulle leef, naamlik die onderwyser, en ander leerders en ouers wat op hul werk kommentaar moes lewer. Hierdie komponente is belangrik in die aktiwiteitsteorie. Met Maier en Ellen se 1956-frustrasieteorie as agtergrond, het die navorser aangeneem dat die projek leerders sal motiveer om beter as in die verlede te doen, of dat dit aggressie, regressie, vermyding of fiksasie sou veroorsaak. Die navorser het 'n kwalitatiewe, interpretatiewe gevallestudie onderneem met 40 graad tien Eerste Addisionele Taal Afrikaans-leerders van 'n privaat skool as deelnemers. Die leerders moes dagboekinskrywings in 'n blog doen. Daarna het hulle narratiewe kommentaar oor hulle ervarings geskryf. Dit is verbatim oorgetik en deur middel van Atlas.ti ontleed. Die meeste leerders se ervarings was positief, baie het aggressief geraak, 'n groot persen-tasie het na 'n normale skryftaak geregresseer, terwyl sommige probeer het om die probleem sover as moontlik te ignoreer. Leerders het hul eie frustrasievlakke verhoog as gevolg van vergeetagtigheid, foute en 'n gebrek aan tydsbestuur en kennis.
Filosofie as aktualiteitsinterpretasie. Marinus Schoeman as denker
Authors: Duvenage P
Published: April 2017

In hierdie bydrae word Marinus Schoeman se denkweg van 42 jaar as filosoof aan die Universiteit van Pretoria geïnterpreteer. Schoeman is 'n tipe Sokrates-figuur en verfrissende voorbeeld in 'n land waar filosowe al te gou in magsposisies belangstel, in plaas daarvan om die grootse roeping van die denke na te volg. Nadat enkele persoonlike opmerkings oor Schoeman gemaak word (deel 1), bestaan hierdie bydrae uit vier dele. In die tweede deel word Schoeman se loopbaan teen die agtergrond van die verskynsel van Afrikaanse filosofie geplaas. Daar word verwys na Schoeman se mentors aan die Universiteit van Pretoria en hoe hulle hom in ten minste sewe sistematiese navorsingsvelde, kenmerkend van Afrikaanse filosofie, ingelei het. Hierna verskuif die fokus na Schoeman self en 'n nabye lees van sy eerste filosofiese werk oor die denke (Kritiese Teorie) van Herbert Marcuse (deel 3). Dit word opgevolg met 'n kritiese waardering van sy vroeëre lees van die Kritiese Teorie en 'n uiters bondige interpretasie van sy latere filosofiese loopbaan wat uiteindelik in die deugde-etiek van Nietzsche en Arendt kulmineer (deel 4). Laastens word daar met enkele opmerkings oor die roeping van die filosoof in Suid-Afrika volstaan en hoe ons daaroor in gesprek met Marinus Schoeman se denkweg kan bly.
Kritiese opmerkings oor ons tyd en sommige van sy gevaarlikste illusies
Authors: Schoeman M
Published: April 2017

Eerstens ondersoek hierdie artikel 'n belangrike verskynsel van ons tyd, naamlik die heroplewing van rewolusionêre bevrydingsbewegings. Basies berus al die rewolusionêre bevrydingsbewegings op minstens twee veronderstellings wat myns insiens albei drogrede-nasies is. Die eerste is die veronderstelling dat ons as mense vry gebore word, en die tweede is die veronderstelling van die nulsom. Beide veronderstellings word krities bespreek. Daar word aangetoon hoe hulle aanleiding gee tot illusies wat n ernstige bedreiging inhou vir die demokrasie en vir beskaafde standaarde in die burgerlike samelewing. Die aandag word veral gerig op die verskynsel van radikaal-Islamitiese terreur en die ideologie van "Derdewêreldisme" oftewel postkoloniale bevryding. Die tweede deel van die artikel handel oor die laat-moderne verskynsel van wat mens kan bestempel as 'n "kultuur van verwerping en onverskilligheid" (beter nog: 'n antikultuur) wat sigself veral in die hoogs ontwikkelde samelewings laat geld. Hierdie antikultuur van onbeskofte en provokerende gedrag kom voort uit n radikale houding van ondankbaarheid, disrespek en 'n totale gebrek aan skroom. Die artikel sluit af met aanduidings van hoe hierdie antikultuur bestry kan word, en wat die voorwaardes vir n beskaafde samelewing sou behels. Filosofie as aktualiteitsanalise - dit beteken dat die filosoof 'n poging aanwend om die tydsgees aan te voel, onder woorde te bring en van vraagtekens te voorsien. "Tydsgees" is 'n vae begrip. Dit is moeilik om dit vas te vang, want as evidensies, d.w.s. as geleefde praktyke, oortuigings, denkgewoontes en denktaboes geld dit meestal as vanselfsprekend, as versweë veronderstellings wat slegs selde tot openbaring kom. Dit het al 'n gemeenplaas geword om te sê dat ons hedendaagse, moderne samelewings gekenmerk word deur die verskynsel van globalisering en sterk beïnvloed word deur die neoliberale lewensopvatting. Dié lewensopvatting berus op die vanselfsprekende aannames van n tegnokratiese en progressiewe elite, wat op elke verstoring van sy wereldbeeld reageer deur die verstorende faktore voor te hou as agterhaalde oorblyfsels uit n oorwonne verlede. Dit lewer n soort sprekerswins op, want dit maak van argumentvoering en debat 'n botsing tussen verteenwoordigers van die toekoms en oorblyfsels van 'n agterhaalde verlede. Wie wil na alles bestempel word as 'n reaksionêr, as 'n struikelblok vir vooruitgang? Ek wil in hierdie bydrae egter nie op die neoliberale gedagtegoed fokus nie. Ek wil eerder die aandag vestig op 'n ander belangrike verskynsel van ons tyd, naamlik die heroplewing van rewolusionêre bevrydingsbewegings. Daarbenewens wil ek, in die tweede plek, stilstaan by die laat-moderne verskynsel van wat mens kan bestempel as 'n "kultuur van verwerping en onverskilligheid" (beter nog: 'n antikultuur) wat sigself veral in die hoogs ontwikkelde samelewings laat geld.
Onvervulbare verlange tussen tradisie en moderniteit: Orhan Pamuk se The Museum of Innocence
Authors: Rossouw J
Published: April 2017

In hierdie artikel word die Turkse wenner van die Nobelprys vir Letterkunde in 2006, Orhan Pamuk, se roman in Engels vertaal as The Museum of Innocence ontleed betreffende die spanning tussen tradisie en moderniteit. Daar word uitgegaan van die tese dat hierdie spanning te wyte is aan die oorhaastige, wilsmatige, sentralistiese, tipies moderne wyse waarop Turkye se groot moderne staatsman, Mustafa Kemal Atatürk, die land tussen 1923 en 1938 gemoderniseer het. Die roman word ontleed aan die hand van twee temas. Die eerste tema is dié van nabootsingsbegeerte waarmee voortgebou word op die werk van Hans Achterhuis en veral René Girard. Hier word aangevoer dat die hoofkarakters in die roman vasgevang is in 'n tussentoestand tussen tradisie en moderniteit, wat hulle probeer ontsnap deur 'n nabootsing van die Westerse moderniteit, met tragiese gevolge. Die tweede tema is dié van verlies en herinneringsvoorwerpe soos dit beslag kry in die hoofkarakter, Kemal, se Museum van Onskuld. Daar word aangetoon dat Pamuk op mimetiese wyse aansluiting vind by die werk van Marcel Proust, maar ook Proust se tema van herinneringsvoorwerpe verder ontgin. Die verskillende fases waarin die Museum van Onskuld beslag kry, word ontleed om uiteindelik tot die gevolgtrekking te kom dat Kemal se onvermoë om anders met die Westerse moderniteit om te gaan, hom dompel in 'n permanente vervanging van die hede en die toekoms met 'n verlore verlede.
Welkom in de woestijn van het dekoloniale filistijnisme. Esthetische reflectie te midden van radicale culturele strijd in Zuid-Afrika
Authors: Pauwels M
Published: April 2017

Dit artikel stelt zich tot doel om de voornaamste theoretische implicaties te doordenken van wat het benoemt als de filistijnse tendens van het radicale dekoloniale cultureel activisme van universiteitstudenten in Zuid-Afrika sinds 2015. Dit verwijst naar het gebruik van extreme, confronterende en gewelddadige strategieën van culturele contestatie zoals het vandaliseren, vernietigen en verwijderen van culturele of artistieke artefacten uit het koloniale of apartheidverleden. Het artikel biedt vooreerst een kritisch overzicht van gangbare theoretische rechtvaardigingen van dergelijke culturele politiek, alsook de belangrijkste tegenwerpingen. Ik voer aan dat deze te kort schieten in het ernstig opvatten en/of fundamenteel doordenken van wat op het spel staat in filistijnse daden jegens artistieke en culturele objecten. Ik beargumenteer dat een meer nuchtere en dialectische conceptualisering vereist is van de filistijnse achterdocht en agressie jegens kunst en cultuur. Als één van de belangrijkste aanzetten tot dergelijke benadering wend ik mij tot Fredric Jamesons notie van de filistijn, die hij articuleerde in zijn interpretatie van het esthetische denken van Theodor Adorno. Alhoewel ik mij distantieer van het neomarxistisch perspectief van Jamesons theorisering, zie ik hierin de mogelijkheid om filistijnisme te begrijpen als reactie tegen wat ik, in verwijzing naar het werk van Jacques Rancière, "esthetische verdelingen" noem, oftewel, opgelegde onderscheidingen en hiërarchieën met betrekking tot de esthetische capaciteiten van verschillende groepen in de samenleving. De filistijnse haat tegenover kunst en cultuur kan dan begrepen worden als een legitieme onvrede met hun medeplichtigheid in het instellen en onderhouden van dergelijke verdelingen. Aldus onderneemt dit artikel om een meer affirmatieve lezing te bieden van de radicale culturele politiek van de huidige studentenactivisten in Zuid-Afrika.
Aluta continua: Accountability and the Domestic Violence Act, 116 of 1998
Authors: VETTEN Lisa
Published: April 2017

In 1998, in an attempt to undo the long-standing neglect of domestic violence,  legislators placed a set of duties on the police in relation to domestic violence and coupled these with a unique system of accountability relations and practices. This articles examines the effect of these in three ways:review both of complaints of misconduct, as well as the station audits conducted in terms of the Domestic Violence Act's prescripts, and analysis of the workings of the Act's accountability mechanisms over time. This shows the Act's system of accountability to have had some success in making domestic violence a policing priority. But this has taken a number of years of interaction across the domains of the political, legal, bureaucratic and the social to accomplish. On this account accountability reveals itself to be a contingent outcome and practice that also takes different forms at different times. It also remains an ambivalent undertaking in relation to domestic violence. While answers may be demanded of the police, oversight of these responses is lodged with an agency possessing limited capacity and weak institutional authority.
Location, location, location: The settling of organised crime in Bedfordview
Authors: Haysom Simone , Shaw Mark
Published: April 2017

Why do ‘crime bosses’ settle in one place and not another? It is an intriguing and under researched question on which little has been written. In South Africa a cluster of individuals associated with organised crime moved into, or were associated with, a particular suburb: Bedfordview, south-east of Johannesburg. The most notorious was Radovan Krejcir, but he himself plugged into an established network of individuals with links to the underworld. This article, based on interviews with people close to high level crime figures or in political or civic leadership roles in Bedfordview, explores why this neighbourhood in particular was chosen. Our analysis suggests that a range of factors coalesced to make Bedfordview, an upper class predominantly white neighbourhood, attractive to organised crime figures. These include a combination of pull factors linked to geography, lifestyle, ethnic and infrastructural reasons that combined in a way that was unique in Johannesburg. Significantly, these were linked with a set of push factors that reflect changes in wider urban development and the upward (and geographic) mobility of a set of ‘businessmen' linked to grey or illegal markets in the city.
More data means better tools for South Africa
Authors: Faull Andrew
Published: April 2017

In February this year, Statistics South Africa (Stats SA) released the findings of the 2015/16 Victims of Crime Survey, and announced that it would release the 2016/17 results in November. Victim surveys, though not without fault, capture valuable data relating to crime, justice and safety, that is not typically collected by criminal justice agencies. Much of their value lies in their ability to identify victimisation trends which may or may not appear in police data, and to gauge perceptions and experiences of criminal justice institutions.
Interview with Lieutenant General Gary Kruser, Deputy National Commissioner, South African Police Service
Authors: Faull Andrew
Published: April 2017

In 2016 the South African Police Service announced that it was going ‘Back-to-Basics’. To leadthis programme, it established a new Management Interventions competency, headed by Lt Gen.Gary Kruser. In February 2017 Dr Johan Burger (ISS) sat down with Kruser to learn more about thenew competency.
A Regional Socio-Geographic Atlas of Secondary Education: Can the “Power of the Territory” Be Surmounted?
Authors: Golubitskiy Aleksey
Published: April 2017

A socio-geographic atlas of secondary education in Kaliningrad Oblast has been made after testing new instruments designed for measuring educational inequality at regional and local levels, its reasons and consequences, and the factors affecting its manifestation. The data for the atlas was obtained from open databases on education quality, information on the region’s attractiveness as a location for real estate investments provided by real estate agents, and the results of measuring the distance of schools from the hubs of social wellbeing. The main assumption at the base of the study is that the influence of environment (factors external to school) on education quality dominates the importance of internal processes. A comparative analysis of the resulting maps of education and territory  quality has revealed not only individual resilient schools and schools that require support but also the low quality zones and socio-geographic anomalies of academic resilience. The article offers methods for studying and overcoming the “curse of the territory” and educational inequality at regional and local levels.
Determining the Linear Range for a Target Protein and REVERT™ Total Protein Stain v1
Authors: Biosciences LI-COR
Published: April 2017

This protocol explains how to use serial dilutions of sample protein to determine the linear ranges of detection for a target and internal loading control, and choose an appropriate amount of sample to load for QWB analysis. Follow the instructions in this protocolif total protein staining of the membrane will be used as the internal loading control for QWB normalization. For normalization with a housekeeping protein, see https://www.protocols.io/view/determining-the-linear-range-for-a-target-protein-g77bzrn. Note: This protocol is intended for use with near-infrared fluorescent Western blots.
Determining the Linear Range for a Target Protein and HKP v1
Authors: Biosciences LI-COR
Published: April 2017

This protocol explains how to use serial dilutions of sample protein to determine the linear ranges of detection for a target and internal loading control, and choose an appropriate amount of sample to load for QWB analysis. Follow the instructions in this section if a housekeeping protein will be used as the internal loading control for QWB normalization. This section also applies to normalization with a pan- specific antibody for analysis of phosphorylation or other post-translational modifications. If you plan to use a total protein stain for normalization, see https://www.protocols.io/view/determining-the-linear-range-for-a-target-protein-g76bzre.
High Efficiency Microfluidic Electrotransformation v1
Authors: A. Garcia Paulo , Zhifei Ge not provided , Laura Kelley not provided , Steven Holcomb not provided , Cullen R. Buie not provided
Published: April 2017

Synthetic biology holds great potential for addressing pressing challenges for mankind and our planet. One technical challenge in tapping into the full potential of synthetic biology is the low efficiency and low throughput of genetic transformation for many types of cells. In this paper, we discuss a novel microfluidic system for improving bacterial electrotransformation efficiency and throughput. Our microfluidic system is comprised of non-uniform constrictions in microchannels to facilitate high electric fields with relatively small applied voltages to induce electroporation. Additionally, the microfluidic device has regions of low electric field to assist in electrophoretic transport of nucleic acids into the cells. The device features hydro- dynamically controlled electric fields that allow cells to experience a time dependent electric field that is otherwise difficult to achieve using standard electronics. Results suggest that transformation efficiency can be increased by ∼4×, while throughput can increase by 100–1000× compared to traditional electroporation cuvettes. This work will enable high-throughput and high efficiency genetic transformation of microbes, fa- cilitating accelerated development of genetically engineered organisms.
Ca2+ imaging (GCaMP6f) from Yao CK et al. (2017) v1
Authors: Yao Chi-Kuang , Yu-Tzu Liu not provided , I-Chi Lee not provided , You-Tung Wang not provided , Ping-Yen Wu not provided
Published: April 2017

This protocol is from 'Flower Ca2+ channel in CME and ADBE' of Yao CK et al. Please see the manuscript for the full method details.
RT-PCR from Yao CK et al. (2017) v1
Authors: Yao Chi-Kuang , Yu-Tzu Liu not provided , I-Chi Lee not provided , You-Tung Wang not provided , Ping-Yen Wu not provided
Published: April 2017

This protocol describes the RT-PCR from 'Flower Ca2+ channel in CME and ADBE' of Yao CK et al. Please see the manuscript for the full method details.
TEM from Yao CK et al. (2017) v1
Authors: Yao Chi-Kuang , Yu-Tzu Liu not provided , I-Chi Lee not provided , You-Tung Wang not provided , Ping-Yen Wu not provided
Published: April 2017

This protocol is from 'Flower Ca2+ channel in CME and ADBE' of Yao CK et al. Please see the manuscript for the full method details.
SypHy imaging and dextran uptake assays from Yao CK et al. (2017) v1
Authors: Yao Chi-Kuang , Yu-Tzu Liu not provided , I-Chi Lee not provided , You-Tung Wang not provided , Ping-Yen Wu not provided
Published: April 2017

This protocol is from 'Flower Ca2+ channel in CME and ADBE' of Yao CK et al. Please see the manuscript for the full method details.
Glass bead transformation of Heterosigma akashiwo v1
Authors: Nanjappa and Kathryn Coyne Deepak
Published: April 2017

This protocol was developed for the glass bead-mediated transformation ofHeterosigma akashiwo. This species does not grow on solid medium, so transformants are grown "in bulk" and should not be considered clonal. We have repeated this protocol with success and have been able to maintain transformants on selection medium for several months.
Electroporation of Heterosigma akashiwo v1
Authors: Nanjappa and Kathryn Coyne Deepak
Published: April 2017

This protocol was developed for transformationof Heterosigma akashiwo by electroporation. This species does not grow on solid medium, so transformants are grown "in bulk" and should not be considered clonal. We have had limited success with this protocol, which may need to be further optimized.
FM1-43 dye uptake (loading/unloading assays) from Yao CK et al. (2017) v1
Authors: Yao Chi-Kuang , Yu-Tzu Liu not provided , I-Chi Lee not provided , You-Tung Wang not provided , Ping-Yen Wu not provided
Published: April 2017

This protocol is from 'Flower Ca2+ channel in CME and ADBE' of Yao CK et al. Please see the manuscript for the full method details.
High Efficiency Microfluidic Electrotransformation v2
Authors: A. Garcia Paulo , Zhifei Ge not provided , Laura Kelley not provided , Steven Holcomb not provided , Cullen R. Buie not provided
Published: April 2017

Synthetic biology holds great potential for addressing pressing challenges for mankind and our planet. One technical challenge in tapping into the full potential of synthetic biology is the low efficiency and low throughput of genetic transformation for many types of cells. In this paper, we discuss a novel microfluidic system for improving bacterial electrotransformation efficiency and throughput. Our microfluidic system is comprised of non-uniform constrictions in microchannels to facilitate high electric fields with relatively small applied voltages to induce electroporation. Additionally, the microfluidic device has regions of low electric field to assist in electrophoretic transport of nucleic acids into the cells. The device features hydro- dynamically controlled electric fields that allow cells to experience a time dependent electric field that is otherwise difficult to achieve using standard electronics. Results suggest that transformation efficiency can be increased by ∼4×, while throughput can increase by 100–1000× compared to traditional electroporation cuvettes. This work will enable high-throughput and high efficiency genetic transformation of microbes, fa- cilitating accelerated development of genetically engineered organisms.
Microfluidic Transient Transfection of B. caudatus with plasmids v1
Authors: Gomaa Fatma , Paulo A. Garcia not provided , Jennifer Delaney not provided , Peter R. Girgius not provided , Cullen R. Buie not provided , Virginia P. Edgcomb not provided
Published: April 2017

We recently developed a continuous flow system to transform microorganisms in high throughput in a microfluidic device (Garcia et al., 2017). This system employs microfluidic channels that contain a bilateral constriction between the inlet and outlet electrode connections (length= 3.0 mm, widthmin= 50μm, widthmax= 2.0mm, and height=100μm). The constriction amplifies the electric field under an applied voltage between the inlet and outlet electrodes to levels sufficiently high to induce electroporation. During B. caudutus transformations, the cells were driven through the microfluidic device at flow rates of 50μL/min and 500μL/min, which correspond to residence times (i.e. pulse durations) of 20ms and 2ms, respectively.Square wave pulses with, for example, 5 ms ON and 5 ms OFF cycles (50% duty cycle) are applied to the microchannel through the dispensing needle. Therefore the cell viability cannot be accurately evaluated since only 50% of the cells experience the electric field. The pulses are delivered from electrodes with alternating polarity between the pulses to reduce electrolytic effects at the electrode-buffer interface (Fig. 1). After flowing through the microchannel (See Supplementary Video 2), each 200 μL cell sample is added to a 1.5 ml Eppendorf tube containing 1 ml of fresh growth media for cell recovery. The applied voltages we evaluated had amplitudes of 250V (Emax=1,500V/cm), 375V (Emax=2,250V/cm), and 500V (Emax=3,000V/cm) for each polarity. The non-uniform constriction in the microfluidic devices generates a variable electric field that is capable of transfecting cells while minimizing exposure to the highest electric field.
Examining Language Related Episodes (LREs) of Arabic as a Second Language (ASL) Learners During Collaborative Writing Activities
Authors: Alwaleedi Mohammed A.
Published: April 2017

Previous studies have increasingly examined the nature of collaborative writing in English as a foreign/second language settings. However, little research has been conducted on its nature in Arabic as a second language (ASL) contexts. This study investigated the nature of 64 students’ Language Related Episodes (LREs) while performing collaborative writing in ASL classrooms. Employing a quasi-experimental design with a mixed methods approach, the frequency and the focus of the students’ LREs in collaborative (experimental) writing groups and in traditional (control) groups were compared. The findings showed that there was a statistically significant difference between the LREs produced by the experimental and the control groups which can be attributed to the collaborative writing approach. In particular, the analysis of LREs per minutes indicated that LREs were more frequent in the experimental group interaction than in control group interaction. In addition, the experimental groups paid more attention to language and were more successful at resolving language related problems than the control ones which may explain the differences in their performance. Specimens of dialogues of both groups are presented to explain their differential performance.