bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Recombinant Herpesvirus of Turkey Laryngotracheitis (rHVT-LT) Vaccine against Genotype VI Canadian Wild-Type Infectious Laryngotracheitis Virus (ILTV) Infection
Authors: Barboza-Solis Catalina , Najimudeen Shahnas M. , Perez-Contreras Ana , Ali Ahmed , Joseph Tomy , King Robin , Ravi Madhu , Peters Delores , Fonseca Kevin , Gagnon Carl A. , van der Meer Frank , Abdul-Careem Mohamed Faizal
Published: December 2021

In Alberta, infectious laryngotracheitis virus (ILTV) infection is endemic in backyard poultry flocks; however, outbreaks are only sporadically observed in commercial flocks. In addition to ILTV vaccine revertant strains, wild-type strains are among the most common causes of infectious laryngotracheitis (ILT). Given the surge in live attenuated vaccine-related outbreaks, the goal of this study was to assess the efficacy of a recombinant herpesvirus of turkey (rHVT-LT) vaccine against a genotype VI Canadian wild-type ILTV infection. One-day-old specific pathogen-free (SPF) White Leghorn chickens were vaccinated with the rHVT-LT vaccine or mock vaccinated. At three weeks of age, half of the vaccinated and the mock-vaccinated animals were challenged. Throughout the experiment, weights were recorded, and feather tips, cloacal and oropharyngeal swabs were collected for ILTV genome quantification. Blood was collected to isolate peripheral blood mononuclear cells (PBMC) and quantify CD4+ and CD8+ T cells. At 14 dpi, the chickens were euthanized, and respiratory tissues were collected to quantify genome loads and histological examination. Results showed that the vaccine failed to decrease the clinical signs at 6 days post-infection. However, it was able to significantly reduce ILTV shedding through the oropharyngeal route. Overall, rHVT-LT produced a partial protection against genotype VI ILTV infection.
Students’ Views on Vaccination against COVID-19 Virus and Trust in Media Information about the Vaccine: The Case of Serbia
Authors: Šiđanin Iva , Njegovan Biljana Ratković , Sokolović Bojana
Published: December 2021

Mass immunization of the citizens of the Republic of Serbia began in January 2021. Information on the significance, manner, advantages and consequences of this process was intensively distributed through all communication channels, with the media playing a key role. According to the data of the official institutions for the public health of Serbia, by July 2021 the lowest percentage of vaccinated population was among those between the ages of 18 and 24—only 15% of this demographic had received the vaccine by this point. Given the low turnout of young people for vaccination, in this paper we investigated the general attitude of students in Serbia, as a special category of young people, towards the vaccine against the COVID-19 virus, as well as their attitude regarding information about vaccination in the media. Research was conducted on a sample of 345 students at the University of Novi Sad. The results of the research showed that 42% of students had not been vaccinated and did not plan to do so, 37.4% had received at least one dose of vaccine and 20.6% had not been vaccinated even though they planned to do so. Students who were vaccinated had more confidence in information provided through media channels than those who were not vaccinated. Therefore, it can be concluded that encouraging students to decide in favor of vaccination against the COVID-19 virus should come from the universities where they study as well as the media.
Longitudinal Characterization of the Mumps-Specific HLA-A2 Restricted T-Cell Response after Mumps Virus Infection
Authors: Lanfermeijer Josien , Nühn Marieke M. , Emmelot Maarten E. , Poelen Martien C. M. , van Els Cécile A. C. M. , Borghans José A. M. , van Baarle Debbie , Kaaijk Patricia , de Wit Jelle
Published: December 2021

Waning of the mumps virus (MuV)-specific humoral response after vaccination has been suggested as a cause for recent mumps outbreaks in vaccinated young adults, although it cannot explain all cases. Moreover, CD8+ T cells may play an important role in the response against MuV; however, little is known about the characteristics and dynamics of the MuV-specific CD8+ T-cell response after MuV infection. Here, we had the opportunity to follow the CD8+ T-cell response to three recently identified HLA-A2*02:01-restricted MuV-specific epitopes from 1.5 to 36 months post-MuV infection in five previously vaccinated and three unvaccinated individuals. The infection-induced CD8+ T-cell response was dominated by T cells specific for the ALDQTDIRV and LLDSSTTRV epitopes, while the response to the GLMEGQIVSV epitope was subdominant. MuV-specific CD8+ T-cell frequencies in the blood declined between 1.5 and 9 months after infection. This decline was not explained by changes in the expression of inhibitory receptors or homing markers. Despite the ongoing changes in the frequencies and phenotype of MuV-specific CD8+ T cells, TCRβ analyses revealed a stable MuV-specific T-cell repertoire over time. These insights in the maintenance of the cellular response against mumps may provide hallmarks for optimizing vaccination strategies towards a long-term cellular memory response.
Attitude towards HPV Vaccination and the Intention to Get Vaccinated among Female University Students in Health Schools in Jordan
Authors: Sallam Malik , Al-Mahzoum Kholoud , Eid Huda , Assaf Areej M. , Abdaljaleel Maram , Al-Abbadi Mousa , Mahafzah Azmi
Published: December 2021

Cervical cancer is a leading cause of morbidity and mortality in women worldwide. The availability of prophylactic vaccines for high-risk types of human papillomavirus (HPV) infection represents an important advancement in the prevention of cervical cancer. In Jordan, the availability of the HPV vaccination is restricted to individuals who are willing to pay. The aim of the current study was to evaluate the willingness and attitude of female university students in health schools/faculties in Jordan to get HPV vaccination and their knowledge about the virus. A self-administered online questionnaire was distributed in October 2021, which comprised 27 items to evaluate HPV knowledge, history of HPV vaccination, intentions to get the HPV vaccine, and the reason(s) behind vaccine refusal for those who rejected vaccination. The study sample comprised 836 participants: medical students (39.7%), pharmacy students (26.0%), dental students (21.2%), and nursing students (13.2%). Only 524 participants had heard of HPV prior to the study (62.7%), of which 48.7% knew about the availability of HPV vaccines. The lowest level of HPV knowledge was observed among nursing students. Only 19/524 students reported a history of HPV vaccination (3.6%). The overall willingness to receive HPV vaccination if provided freely was 75.0%, while only 16.0% were willing to pay for the vaccine. The most common reason for HPV vaccine rejection was the perceived low risk to get HPV infection. Significantly higher intentions to get HPV vaccination were found among older participants and medical students. The embrace of vaccine conspiracy beliefs was associated with a significantly less willingness to get the HPV vaccination (p < 0.001). Dependence on the internet/social media as the source of HPV knowledge was associated with a significantly lower intention to get HPV vaccination (p = 0.002). The coverage of the HPV vaccination among female university students in health schools in Jordan appeared extremely low; however, three-fourths of the students who had heard of HPV were willing to receive the HPV vaccination if provided freely. Complacency appeared as a major factor for HPV vaccine rejection. Increasing the levels of knowledge and awareness of HPV infection and its association with cervical cancer through reliable sources is recommended. This can be helpful for the individual benefit of the students besides the potentially positive role they can play in community education. Countering vaccine conspiracy beliefs with proper education and awareness programs can be helpful to appraise the role of HPV vaccines in cancer prevention.
COVID-19 Vaccine Booster Hesitancy (VBH) of Healthcare Workers in Czechia: National Cross-Sectional Study
Authors: Klugar Miloslav , Riad Abanoub , Mohanan Lekshmi , Pokorná Andrea
Published: December 2021

The emerging SARS-CoV-2 variants and waning vaccine-elicited immunity are two public health challenges that occurred simultaneously and synergistically during the summer of 2021 and led to a surging demand for COVID-19 vaccine booster dose (BD) rollout. This study aimed to evaluate the COVID-19 vaccine booster hesitancy (VBH) among Czech healthcare workers to explore the potential determinants of VBH. A national cross-sectional survey-based study was carried out between 3 and 11 November 2021, using an online self-administered questionnaire (SAQ) that explored the participants’ demographic characteristics, COVID-19 infection and vaccine anamneses, willingness to receive COVID-19 vaccine BD, and the psychosocial drivers of VBH. A total of 3454 HCW properly responded to the online SAQ, of which 80.9% were females, 30.3% were medical professionals, and 50.5% were ≤47 years old. Most of the participants were already inoculated against SARS-CoV-2 (95.2%), and BTN162b2 was the most commonly administered vaccine (90.7%). As the study sample was planned to represent the target population, it revealed a high level of BD acceptance (71.3%) among Czech HCW, while 12.2% were still hesitant and 16.6% were against the currently available BD. These results are consistent with other recent results from central Europe. Medical professional, male, and older participants were more likely to accept BD rather than allied health professional, female, and younger participants. The BDs’ perceived effectiveness against severe illness, symptomatic infection, and community transmission was a significant and strong predictor for BD acceptance, while the effectiveness against the circulating variants was not that important for our target population. The BDs’ perceived safety and ethical dilemmas of vaccine justice should be addressed sufficiently while communicating with HCW and other population groups. The altruistic reasons for BD acceptance, i.e., family protection, patient protection, and community health protection, underpin the recommendation of postponing the COVID-19 vaccine mandating in favour of stressing these altruistic concerns amid public health messaging.
Masculinizing Effects of Chrysin-Loaded Poloxamer Micelles on Siamese Fighting Fish
Authors: Khumpirapang Nattakanwadee , Sassa-deepaeng Tanongsak , Suknuntha Krit , Anuchapreeda Songyot , Okonogi Siriporn
Published: December 2021

Siamese fighting fish (Betta splendens) are freshwater fish that are commonly found in Thailand and other Southeast Asian countries. In the present study, chrysin-loaded polymeric micelles (CPs) were developed and investigated for the masculinizing effects, survival rate, growth indices, and toxicity on Siamese fighting fish. CPs were prepared using a poloxamer. The micelle system of CPs that were formed at a chrysin-to-polymer ratio of 1:2 was found to be the most suitable monodispersed system and exhibited a nanosized diameter (74.2 ± 1.6 nm) with a narrow size distribution (0.288 ± 0.012). In vivo studies were performed using Siamese fighting fish larvae as animal models. In the in vivo toxicity study, the fish larvae were immersed in aqueous systems containing CPs that had five different chrysin concentrations of 1, 10, 100, 1000, and 10,000 ng/mL for 24, 48, and 72 h. Blank polymeric micelles and water were used as controls. The in vivo masculinization effect of CPs with different chrysin concentrations on the fish larvae was evaluated after 5 weeks of exposure. The results demonstrated that CPs with a chrysin concentration of 1000 ng/mL showed a masculinization effect of 94.59 ± 2.76% with a high fish larvae survival rate of 72.45 ± 5.09% and low toxicity. It was concluded that the developed CPs had a significant effect on the sex reversal of Siamese fighting fish larvae with a high survival rate.
Immunophenotyping of an Unusual Mixed-Type Extraskeletal Osteosarcoma in a Dog
Authors: Kim Hyo-Sung , Kim Han-Jun , Hwang Hyun-Jeong , Ahn Jong-Hyun , Do Sun-Hee
Published: December 2021

A 6-year-old female Maltese dog presented with a cervical mass without pain. The tumor was surrounded by a thick fibrous tissue and consisted of an osteoid matrix with osteoblasts and two distinct areas: a mesenchymal cell-rich lesion with numerous multinucleated giant cells and a chondroid matrix-rich lesion. The tumor cells exhibited heterogeneous protein expression, including a positive expression of vimentin, cytokeratin, RANKL, CRLR, SOX9, and collagen 2, and was diagnosed as extraskeletal osteosarcoma. Despite its malignancy, the dog showed no sign of recurrence or metastasis three months after the resection. Further analysis of the tumor cells revealed a high expression of proliferation- and metastasis-related biomarkers in the absence of angiogenesis-related biomarkers, suggesting that the lack of angiogenesis and the elevated tumor-associated fibrosis resulted in a hypoxic tumor microenvironment and prevented metastasis.
Infrared Thermography Evaluation of Feet Temperature and Its Association with Claw Lengths and Anisodactylia in Purebred Sows of Three Greek Herds
Authors: Kroustallas Fotios G. , Papadopoulos Georgios A. , Chalvatzi Sofia , Skampardonis Vasilis , Leontides Leonidas , Fortomaris Paschalis
Published: December 2021

The aim of the study was to investigate the associations of lower feet temperature with claw lengths measurements in purebred sows. In total 22, 19 and 45 multiparous sows in three herds A, B and C of PIC, DANBRED and TOPIGS genetic lines respectively participated in the study. Mean parity was 2.5, 2.3 and 3.0 for sows from herds A, B and C respectively. Measurements were made during the periparturient period. Infrared temperature distribution was measured in carpus/tarsus, upper-lower metacarpi/metatarsi and phalanges (IRT1, IRT2, IRT3 and IRT4 respectively). In addition, dorsal, diagonal, heel–sole and dew claw lengths of medial and lateral claw were measured and the difference in dorsal claw length between medial and lateral claw (anisodactylia) was calculated in all four feet. Differences between herds regarding IRT and claw length measurements were analyzed with one-way ANOVA with herds as a fixed factor. Correlations between IRT and claw length measurements in each foot including data from all herds were evaluated using the Pearson’s correlation test. Maximum IRT1 to 4 in almost all rear feet, differed significantly between herds, being lower in sows of herd C than A and B (p < 0.05). Claw lengths of all feet were lower in herd C than those of A and B (p < 0.05). Anisodactylia, differed significantly only in rear feet between herds been higher in herd A than C and B (p < 0.05). In all sows, claw lengths and rear feet anisodactylia were positively correlated with maximum IRT1 to IRT4 (p < 0.05). According to the results, IRT temperature distribution of lower feet of purebred sows of different genetic lines were positive correlated with claw lengths measurements and anisodactylia. Collectively, measuring IRT temperature of lower feet of sows with mobile IRT device could be used as an additional tool towards monitoring feet and claw health.
Exposure of Domestic Cats to Distinct Ehrlichia canis TRP Genotypes
Authors: Braga Ísis , Taques Isis , Grontoski Estefânia , de Oliveira Dias Ingrid , Pereira Nathalia , de Souza Ramos Dirceu , Dantas-Torres Filipe , de Aguiar Daniel
Published: December 2021

Cats naturally exposed to Ehrlichia canis have been described in different regions of the world, but little is known about the genotypes associated with infection in these animals. To detect E. canis-specific antibodies and investigate the E. canis TRP genotypes in cats, serum samples from 76 domestic cats reactive to crude E. canis antigens by the indirect fluorescence antibody test (IFAT) were analyzed by ELISA, using E. canis-specific peptides (i.e., TRP19 and TRP36 /BR/US/CR). Of these, 25 (32.9%) cats reacted to at least one TRP peptide, confirming their specific exposure to E. canis. Eighteen (23.7%) cats reacted to TRP19, 15 (19.8%) to BRTRP36, and 11 (14.5%) to USTRP36, but none of them reacted to CRTRP36. Eight (10.5%) cats reacted to TRP19 but not to any TRP36 genotype, demonstrating the possible existence of a new E. canis genotype infecting felines. Nevertheless, this study provides the first report of anti-E. canis-specific antibodies in domestic cats.
Legacy and Emerging Pollutants in an Urban River Stretch and Effects on the Bacterioplankton Community
Authors: Visca Andrea , Barra Caracciolo Anna , Grenni Paola , Rolando Ludovica , Mariani Livia , Rauseo Jasmin , Spataro Francesca , Monostory Katalin , Sperlagh Beata , Patrolecco Luisa
Published: December 2021

River contamination is due to a chemical mixture of point and diffuse pollution, which can compromise water quality. Polycyclic Aromatic Hydrocarbons (PAHs) and emerging compounds such as pharmaceuticals and antibiotics are frequently found in rivers flowing through big cities. This work evaluated the presence of fifteen priority PAHs, eight pharmaceuticals including the antibiotics ciprofloxacin (CIP) and sulfamethoxazole (SMX), together with their main antibiotic resistant genes (ARGs) and the structure of the natural bacterioplankton community, in an urbanized stretch of the river Danube. SMX and diclofenac were the most abundant chemicals found (up to 20 ng/L). ARGs were also found to be detected as ubiquitous contaminants. A principal component analysis of the overall microbiological and chemical data revealed which contaminants were correlated with the presence of certain bacterial groups. The highest concentrations of naphthalene were associated with Deltaproteobacteria and intI1 gene. Overall, the most contaminated site was inside the city and located immediately downstream of a wastewater treatment plant. However, both the sampling points before the river reached the city and in its southern suburban area were still affected by emerging and legacy contamination. The diffuse presence of antibiotics and ARGs causes particular concern because the river water is used for drinking purposes.
Combined Application of Ozone and Hydrogen Peroxide to Degrade Diesel Contaminants in Soil and Groundwater
Authors: Chen Wen-Yu , Pokhrel Prakash , Wang Ying-Shun , Lin Sheng-Lung , Liu Min-Hsin
Published: December 2021

Environmental pollution has been a major concern in recent times, and soil and groundwater pollution are areas which have received particular focus. This has led to the development of various remediation techniques such as excavation, soil vapor extraction, bioremediation, chemical oxidation, and so on. Among all remediation techniques, chemical oxidation has been proven to be the most effective and feasible technique around the world. In this study, various combinations of ozone and hydrogen peroxide were used to treat diesel-contaminated soil and groundwater in an experimental setup. Experimental soil and groundwater were prepared with properties similar to the contaminated soil. An ozone generator and a pump injection system were deployed for combining ozone and hydrogen peroxide. Five different experiment batches were prepared based on the hydrogen peroxide concentration and its ratio to the soil. The diesel concentration in the water dropped from 300 mg/L to 7 mg/L in the first hour of treatment, which dropped below the detection limit (0.01 mg/L) thereafter. Similarly, 63.9% degradation was achieved with the combined sparging of ozone and hydrogen peroxide in the soil. Ozone combined with 7% hydrogen peroxide was the most promising combination for removing the contaminants. In addition, this research explored the hydroxyl radical conversion rate of ozone and the perozone, the difference in order of magnitude is greater than one which shows that the perozone has better oxidation capacity than ozone only. The findings of this study show that combining ozone with hydrogen peroxide is a competent and feasible onsite remediation method for diesel contaminants in soil and groundwater. Thus, this method can be applied in local gas stations, accidental spillage sites, and small-scale refineries for onsite treatment in a cost-effective and technically sound way within a short time span.
Research on Flood Discharge and Energy Dissipation of a Tunnel Group Layout for a Super-High Rockfill Dam in a High-Altitude Region
Authors: Zhang Haichao , Zhang Luchen , Wu Shiqiang , Wang Fuming , Zhan Zhenggang , Zheng Xueyu , Zhang Heng , Bao Wei
Published: December 2021

Under the condition of a large dip angle between the flood discharging structure axis and the downstream cushion pool centerline, the downstream flow connection for the discharging tunnel group is poor, and the lower air pressure in high-altitude areas increases its influence on the trajectory distance of the nappe, further increasing the difficulty of predicting the flood discharge and energy dissipation layout. Based on the RM hydropower project with the world’s highest earth-rockfill dam, this paper studies the problem of a large included angle flip energy dissipation layout of a tunnel group flood discharge using the method of the overall hydraulic physical model test. The test results show that the conventional flip outlet mode has a long nappe falling point, a serious shortage of effective energy dissipation space, a large dynamic hydraulic pressure impact peak value on the bottom slab and side wall of the plunge pool, a poor flow connection between the outlet of the plunge pool and the downstream river channel, and a low energy dissipation rate. Considering the influence of a low-pressure environment, when the “transverse diffusion and downward incidence” outflow is adopted, the nappe falling point shrinks by 11 m, the energy dissipation form of the plunge pool is greatly improved, the effective energy dissipation space is increased by 159%, the RMS of the maximum fluctuating pressure is reduced by 74%, the outflow is smoothly connected with the downstream river, the energy dissipation rate is increased by 0.8%, and the protection range of flood discharge atomization is significantly reduced. This effectively solves the safety problems of large included angle discharge return channels and the energy dissipation and erosion prevention of super-high rockfill dams.
Optimization of Nitrogen Fertilizer Application with Climate-Smart Agriculture in the North China Plain
Authors: Chen Jinsai , Wang Guangshuai , Hamani Abdoul Kader Mounkaila , Amin Abubakar Sunusi , Sun Weihao , Zhang Yingying , Liu Zhandong , Gao Yang
Published: December 2021

Long−term excessive nitrogen fertilizer input has resulted in several environmental problems, including an increase in N2O emissions and the aggravation of nitrate leaching; monitoring nitrogen fertilizer is crucial for maize with high yield. This study aimed to optimize the amount of nitrogen applied to maize by Climate−Smart Agriculture (CSA) so as to continuously improve agricultural productivity and reduce or eliminate N2O emissions as much as possible. Field experiments with a completely randomized design were conducted to examine the effects of six nitrogen treatments (N application levels of 0, 120, 180, 240, 300, 360 kg·ha−1, respectively) on N2O emissions, residual concentration of nitrate and ammonium nitrogen, maize yield, and nitrogen utilization efficiency in 2018 and 2019. The results indicated that the residual concentration of nitrate nitrogen (NO3-−N) in the two seasons significantly increased; N2O emissions significantly increased, and the nitrogen fertilizer agronomic efficiency and partial productivity of maize fell dramatically as the nitrogen application rate increased. The maize grain yield rose when the N application amount was raised (N application amount <300 kg·ha−1) but decreased when the N application amount > 300 kg·ha−1. An increase in the nitrogen application rate can decrease nitrogen use efficiency, increase soil NO3-−N residual, and N2O emissions. Reasonable nitrogen application can increase maize yield and reduce N2O emissions and be conducive to improving nitrogen use efficiency. By considering summer maize yield, nitrogen use efficiency, and farmland ecological environment, 173.94~178.34 kg N kg·ha−1 could be utilized as the nitrogen threshold for summer maize in the North China Plain.
Geomorphological Changes of a Migrating Sandbank: Multidecadal Analysis as a Tool for Managing Conflicts in Coastal Use
Authors: Bezzi Annelore , Casagrande Giulia , Fracaros Saverio , Martinucci Davide , Pillon Simone , Sponza Stefano , Bratus Antonio , Fattor Fabrizio , Fontolan Giorgio
Published: December 2021

While beach erosion and sand loss are typically of great concern to the tourism industry, managing rapid morphological changes linked to large amounts of moving sediments is the challenge facing Grado, an important seaside resort in the northern Adriatic, Italy. The cause of the unusual management conflict is the presence of the Mula di Muggia Bank, a nearshore depositional system made up of relict and active migrating sandbanks extending up to 2 km seawards from the touristic beachfront. A reconstruction of the morpho-sedimentary evolution of the coastal system over a 200-year period was done using a large dataset which includes historical cartography, topographic maps, aerial photos and topo-bathymetric surveys. The results show the growth of a significant urban development aimed at creating a tourist destination by occupying the waterfront along fetch-limited coastal tracts with very shallow water and scarce hydrodynamics. Furthermore, a number of sandy dynamic landforms (longshore migrating bars, a bypass corridor, an ebb-tidal delta) and accumulation zones attest to a sediment excess which can be mostly attributed to the eastern river supplies. The progressive constant migration rate of 12.6 my−1 allowed the bank to induce the expansion of the low-energy silty backbarrier environment, characterised by abundant seagrass meadows a short distance directly in front of the tourist beaches of Grado. As a result of historical analysis and more current observations, areas with diverse morphosedimentary features and with varying tourist/recreational, ecological, and conservation values have been identified. These can be considered as basic units for future accurate planning and re-evaluation of coastal management choices to balance environmental protection and tourist use. A soft coastal defence approach is proposed which includes either the preservation of specific environments or the proper use of excess sand for beach nourishment via periodic dredging or sediment bypassing.
Quantile-Based Hydrological Modelling
Authors: Tyralis Hristos , Papacharalampous Georgia
Published: December 2021

Predictive uncertainty in hydrological modelling is quantified by using post-processing or Bayesian-based methods. The former methods are not straightforward and the latter ones are not distribution-free (i.e., assumptions on the probability distribution of the hydrological model’s output are necessary). To alleviate possible limitations related to these specific attributes, in this work we propose the calibration of the hydrological model by using the quantile loss function. By following this methodological approach, one can directly simulate pre-specified quantiles of the predictive distribution of streamflow. As a proof of concept, we apply our method in the frameworks of three hydrological models to 511 river basins in the contiguous US. We illustrate the predictive quantiles and show how an honest assessment of the predictive performance of the hydrological models can be made by using proper scoring rules. We believe that our method can help towards advancing the field of hydrological uncertainty.
Generation of Homogeneous Slope Units Using a Novel Object-Oriented Multi-Resolution Segmentation Method
Authors: Li Yange , He Jianhua , Chen Fang , Han Zheng , Wang Weidong , Chen Guangqi , Huang Jianling
Published: December 2021

The generation of map units is a fundamental step for an appropriate assessment of landslide susceptibility. Recent studies have indicated that the terrain relief-based slope units perform better in homogeneity compared with the grid units. However, it is difficult at present to generate high-precision and high-matching slope units by traditional methods. The problem commonly concentrates in the plain areas without obvious terrain reliefs and the junction of sudden changes in terrain. In this paper, we propose a novel object-oriented segmentation method for generating homogeneous slope units. Herein, the multi-resolution segmentation algorithm in the image processing field is introduced, enabling the integration of terrain boundary conditions and image segmentation conditions in slope units. In order to illustrate the performances of the proposed method, Kitakyushu region in Japan is selected as a case study. The results show that the proposed method generates satisfactory slope units that satisfactorily reproduce the actual terrain relief, with the best within-unit and between-unit homogeneities compared with the previous methods, in particular at the plain areas. We also verify the effectiveness of the presented method through the sensitivity analysis using different resolutions of digital elevation models (DEMs) data of the region. It is reported that the presented approach is notably advanced in the requirements of the quality of DEM data, as the presented approach is less sensitive to DEM spatial resolution compared with other available methods.
User Satisfaction and Crowding at Florida Keys National Marine Sanctuary
Authors: Hurtado Melissa , Burns Robert C. , Andrew Ross G. , Schwarzmann Danielle , Moreira Jasmine Cardozo
Published: December 2021

Effective management and monitoring of recreational use is fundamental in marine protected areas. The primary purpose of this study is to examine user’s satisfaction levels, crowding levels and the relationship between them at Florida Keys National Marine Sanctuary. Online surveys were sent via Qualtrics to email addresses obtained from the state of Florida during the summer of 2020. The results showed that the respondents felt very satisfied and only slightly crowded while snorkeling or scuba diving during their most recent 2019 trip in the Florida Keys, although satisfaction was still negatively impacted by crowding. An analysis of variance (ANOVA) showed that satisfaction levels were significantly lower in users who saw more people than they expected. A linear regression indicated significantly lower satisfaction with increasing age. Furthermore, a multiple regression showed that experiencing natural surroundings has a significant positive relationship in overall trip satisfaction, i.e., users that are more satisfied experiencing natural surroundings are also likely to be more satisfied with their overall trip satisfaction. This study suggests that the convergence of social and natural resource research and practices can help managers to create better policies that will maximize human benefits from, and minimize human pressures on, ocean and coastal environments.
Dynamics of Mid-Channel Bar during Different Impoundment Periods of the Three Gorges Reservoir Area in China
Authors: Tang Qingqing , Tan Daming , Ji Yongyue , Yan Lingyun , Zeng Sidong , Chen Qiao , Wu Shengjun , Chen Jilong
Published: December 2021

The dynamics of the mid-channel bars (MCBs) in the Three Gorges Reservoir (TGR) were substantially impacted by the large water-level changes due to the impoundments of the TGR. However, it is still not clear how the morphology of the MCBs changed under the influence of water level and hydrological regime changes induced by the impoundments and operation of the TGR. In this work, the MCBs in the TGR were retrieved using Landsat remote sensing images from 1989 to 2019, and the spatio-temporal variations in the number, area, morphology and location of the MCBs during different impoundment periods were investigated. The results showed that the number and area of MCBs changed dramatically with water-level changes, and the changes were dominated by MCBs with an area less than 0.03 km2 and larger than 1 km2. The area of MCBs decreased progressively with the rising water level, and the number generally showed a decreasing trend, with the minimum number occurring at the third stage when the water level reached 139 m, resulting in the maximum average area at this period. The ratio of length to width of the MCBs generally decreased with the changes in hydrological and sediment regimes, leading to a shape adjustment from narrow–long to relatively short–round with the rising of the water level. The water impoundments of the TGR led to the migration of the dominant area from the upper section to the middle section of the TGR and resulted in a more even distribution of MCBs in the TGR. The results improve our understanding of the mechanisms of the development of MCBs in the TGR under the influence of water impoundment coupled with the annually cyclic hydrological regime and longer periods of inundation and exposure.
Case Study of Urban Flood Inundation—Impact of Temporal Variability in Rainfall Events
Authors: Li Ting , Lee Gyuwon , Kim Gwangseob
Published: December 2021

This study aimed to calculate and analyze total overflows that accumulate in urban manholes in the target drainage basin of Samsung-dong, Seoul in heavy rainfall events with different temporal distribution characteristics, using the EPA’s Storm Water Management Model (EPA-SWMM model). Inundation behaviors were analyzed using the two-dimensional flood model (FLO-2D). The extreme rainfall events were produced using different exceedance probability Huff distributions for different durations and return periods, such as from 1 to 3 h and 10 years, 50 years, 80 years, 100 years, respectively. The inundation model was validated using the actual flood observations on 21 September 2010 in the Samsung-dong drainage basin. The total overflow amount showed considerable differences according to the different time distribution characteristics, such as the temporal location of the storm peak and the concentration level of the storm. Furthermore, the inundation behaviors were also related to the temporal characteristics of storms. The results illustrated that the consideration of the temporal distribution characteristics of extreme rainfall events is essential for an accurate understanding of the rainfall–runoff response and inundation behavior in urban drainage basins.
Depth–Duration–Frequency Relationship Model of Extreme Precipitation in Flood Risk Assessment in the Upper Vistula Basin
Authors: Markiewicz Iwona
Published: December 2021

The Upper Vistula Basin is a flood-prone region in the summer season (May–October) due to intensive rainfall. From the point of view of water management, it is particularly important to assess the variability in this main factor of flood risk, as well as to establish the depth–duration–frequency (DDF) relationship for maximum precipitation, this having not yet been derived for the region. The analysis of a 68-year (1951–2018) data series of summer maximum precipitation collected by 11 meteorological stations showed the series’ stationarity, which supports the conclusion that there is no increase in the risk of rainfall floods due to the intensification of extreme precipitation. A new approach is proposed for the determination of the DDF relationship, where the best-fitted distribution for each station is selected from among the set of candidate distributions, instead of adopting one fixed distribution for all stations. This approach increases the accuracy of the DDF relationships for individual stations as compared to the commonly used approach. In particular, the traditionally used Gumbel distribution turns out to be not well fitted to the investigated data series, and the advantage of the recently popular GEV distribution is not significant.
Epiphyton in Agricultural Streams: Structural Control and Comparison to Epilithon
Authors: Wijewardene Lishani , Wu Naicheng , Giménez-Grau Pau , Holmboe Cecilie , Fohrer Nicola , Baattrup-Pedersen Annette , Riis Tenna
Published: December 2021

Stream biofilms play an important role in the structure, functioning, and integrity of agricultural streams. In many lowland streams, macrophyte vegetation is abundant and functions as an important substrate for biofilm (epiphyton) in addition to the gravel and stone substrate for epilithon on the stream bed. We expect that reach-scale habitat conditions in streams (e.g., nutrient availability, hydraulic conditions) affect the epiphyton and epilithon biomass and composition, and that this effect will be substrate-specific (macrophytes and stones). The objectives of our study were (i) to describe concurrent changes in epiphyton and epilithon biomass and composition over a year in agricultural streams, and (ii) to determine the substrate specific reach-scale habitat drivers for the epiphyton and epilithon structure. We monitored epiphyton and epilithon biofilm biomass and composition at three-week intervals and reach-scale environmental conditions daily during a year for two agricultural steams. The results showed that epiphyton and epilithon communities differed in biomass, having high substrate specific biomass in epilithon compared to epiphyton. Epiphyton was mainly composed of diatom and green algae, while cyanobacteria were more important in epilithon, and the diatom species composition varied between the two biofilm types. Epiphyton structural properties were less influenced by reach-scale hydrology and nutrient availability compared to epilithon. The overall explanatory power of the measured environmental variables was low, probably due to micro-scale habitat effects and interactive processes within stream biofilms. Knowledge of biofilm control in agricultural streams is important in order to improve management strategies, and future studies should improve the understanding of micro-scale habitat conditions, interactive relationships within biofilms and between the biofilm and the substrates.
Sustainable Treatment of Food Industry Wastewater Using Membrane Technology: A Short Review
Authors: Pervez Md. Nahid , Mishu Monira Rahman , Stylios George K. , Hasan Shadi W. , Zhao Yaping , Cai Yingjie , Zarra Tiziano , Belgiorno Vincenzo , Naddeo Vincenzo
Published: December 2021

Water is needed for food processing facilities to carry out a number of tasks, including moving goods, washing, processing, and cleaning operations. This causes them to produce wastewater effluent, and they are typically undesirable since it contains a high volume of suspended solids, bacteria, dyestuffs, salts, oils, fats, chemical oxygen demand and biological oxygen demand. Therefore, treatment of food industry wastewater effluent is critical in improving process conditions, socio-economic benefits and our environmental. This short review summarizes the role of available membrane technologies that have been employed for food wastewater treatment and analyse their performance. Particularly, electrospun nanofiber membrane technology is revealed as an emerging membrane science and technology area producing materials of increasing performance and effectiveness in treating wastewater. This review reveals the challenges and perspectives that will assist in treating the food industry wastewater by developing novel membrane technologies.
Qualitative and Quantitative Beach Cleanliness Assessment to Support Marine Litter Management in Tropical Destinations
Authors: Botero Camilo M. , Tamayo Diana , Zielinski Seweryn , Anfuso Giorgio
Published: December 2021

In addition to its ecological and recreational relevance, beach cleanliness is also one of the five most important aspects (i.e., the “Big Five”) for beach visitors around the world. Nonetheless, few efforts have been carried out to guide the sound management of this rising issue. This paper presents a quantitative and qualitative method to assess the level of beach cleanliness, in order to allow managers to focus their attention on the environmental management of the most frequent and relevant types of litter in tropical areas. In a first step, a survey on users’ perception was applied to 361 beachgoers in eight beaches in Colombia to identify the most relevant types of beach litter and the weighting factors to obtain quantitative calculations. In a second step, the thirteen categories of litter identified were analyzed in relation to beach cleanliness, origin of litter, and environmental impact, to define its individual weighting importance. Some categories were also selected according to particularities of tropical countries, such as the presence of abundant natural vegetation debris and gross polystyrene items, largely transported by rivers in great quantities. In a third step, the method was then tested at eight beaches in the Caribbean coast of Colombia through a period of four months, during which 192 transect samplings were carried out. The qualitative module of the proposed technique, named Beach Litter Assessment Technique—Qualitative and Quantitative (BLAT-QQ), identified the main types of beach litter and the quantitative module gave an overview on the state of beach cleanliness. The method is demonstrated to be quick and effective in diagnosing beach cleanliness, providing a simple instrument to carry out sound environmental management actions in coastal destinations.
Effect of the Recycling Process on Drinking Water Treatment: Evaluation Based on Fluorescence EEM Analysis Using the Peak-Picking Technique and Self-Organizing Map
Authors: Hudori Hudori , Rosadi Maulana Yusup , Yamada Toshiro , Bhat Sartaj Ahmad , Li Fusheng
Published: December 2021

The recycling process is applied in many water treatment plants (WTPs), although this process can lead to adverse effects. The effect of the recycling process on the characteristics of dissolved organic matter was evaluated based on a fluorescence excitation-emission matrix using the peak-picking technique and self-organizing map (SOM). In this study, an evaluation of two WTPs, one with and one without a recycling system, was carried out. Both WTPs show moderate efficiency during the coagulation–flocculation process in removing DOC, fulvic acid-like, humic acid-like, and tryptophan-like substances. The recycling process causes increased values of fulvic acid-like, humic acid-like, and tryptophan-like substances and specific ultraviolet absorbance (SUVA) after the filtration process of about 31.0%, 35.7%, 22.2%, and 6%, respectively. Meanwhile, the WTP without recycling showed a reduction in the level of fulvic acid-like, humic acid-like, and tryptophan-like substances and SUVA by 23.3%, 52.9%, 27.8%, and 21.1%, respectively. Moreover, SOM analysis based on the peak-picking technique can determine differences in sample clusters due to the recycling process.
A Machine Learning Framework for Olive Farms Profit Prediction
Authors: Christias Panagiotis , Mocanu Mariana
Published: December 2021

Agricultural systems are constantly stressed due to higher demands for products. Consequently, water resources consumed on irrigation are increased. In combination with the climatic change, those are major obstacles to maintaining sustainable development, especially in a semi-arid land. This paper presents an end-to-end Machine Learning framework for predicting the potential profit from olive farms. The objective is to estimate the optimal economic gain while preserving water resources on irrigation by considering various related factors such as climatic conditions, crop management practices, soil characteristics, and crop yield. The case study focuses on olive tree farms located on the Hellenic Island of Crete. Real data from the farms and the weather in the area will be used. The target is to build a framework that will preprocess input data, compare the results among a group of Machine Learning algorithms and propose the best-predicted value of economic profit. Various aspects during this process will be thoroughly examined such as the bias-variance tradeoff and the problem of overfitting, data transforms, feature engineering and selection, ensemble methods as well as pursuing optimal resampling towards better model accuracy. Results indicated that through data preprocessing and resampling, Machine Learning algorithms performance is enhanced. Ultimately, prediction accuracy and reliability are greatly improved compared to algorithms’ performances without the framework’s operation.
Nitrogen Removal by an Anaerobic Iron-Dependent Ammonium Oxidation (Feammox) Enrichment: Potential for Wastewater Treatment
Authors: Rodríguez Carolina , Cisternas Jaime , Serrano Jennyfer , Leiva Eduardo
Published: December 2021

Nitrogen pollution in water is a growing concern. Anthropogenic activities have increased the amount of nitrogen released into watercourses, which harms human health and the environment, and causes serious problems, such as eutrophication. Feammox is a recently discovered biological pathway associated with the nitrogen cycle that has gained scientific interest. This process couples anaerobic ammonium oxidation with iron reduction. This work presents a study on the Feammox mechanism from the enrichment of an activated sludge obtained from a sewage treatment plant. The enrichment was carried out at neutral pH to study the N2 pathway, that is, the Feammox process with the oxidation of ammonium (NH4+) directly to N2. In addition, different sources of iron were studied: iron chloride (FeCl3); ferrihydrite; and goethite. The characterization of the sludge showed the genes associated with ammonia monooxygenase, nitrate and nitrite reductases processes, along with relevant microbial species. The enrichment, carried out for 42 days and monitored every 14 days, showed that FeCl3 as a source of Fe was more effective for the coupled process of oxidation of NH4+ and the reduction of Fe(III) to Fe(II). At the end of the enrichment period, a removal of 31% and 32.2% of NH4+, and an increase in Fe(II) concentration by 52.4 and 63.9 times regarding the initial value were achieved in aerobic and anaerobic sludge, respectively. This study provides information on the potential of Feammox in the removal of N from wastewater, and the oxidation/reduction yields in the initial enrichment phase.
Recovery of Alkaline Earth Metals from Desalination Brine for Carbon Capture and Sodium Removal
Authors: Lee Cheng-Han , Chen Pin-Han , Chen Wei-Sheng
Published: December 2021

Because carbon dioxide adsorbs the radiation from the Sun and the Earth’s surface, global warming has become a severe problem in this century. Global warming causes many environmental problems such as heatwave, desertification, and erratic rainfall. Above all, erratic rainfall makes people have insufficient freshwater. To solve this problem, desalination technology has been developed in many countries. Although desalination technology can provide freshwater, it produces brine as well (producing 1 L of freshwater would result in 1 L of brine). The brine will decrease the dissolved oxygen in the sea and affect the organism’s habitat. In this study, magnesium and calcium from desalination brine were recovered in the form of magnesium hydroxide and calcium hydroxide by adjusting the pH value for carbon capture and sodium removal. Magnesium hydroxide would turn into magnesium carbonate through contacting CO2 in saturated amine carriers. Calcium hydroxide was added to the brine and reacted with CO2 (modified Solvay process). Sodium in brine would then be precipitated in the form of sodium bicarbonate. After removing sodium, brine can be released back into the ocean, or other valuable metals can be extracted from brine without the side effect of sodium. The results revealed that 288 K of 3-Amino-1-propanol could capture 15 L (26.9 g) of CO2 and that 25 g/L of Ca(OH)2 at 288 K was the optimal parameter to remove 7000 ppm sodium and adsorb 16 L (28.7 g) of CO2 in the modified Solvay process. In a nutshell, this research aims to simultaneously treat the issue of CO2 emission and desalination brine by combining the amines carrier method and the modified Solvay process.