bluuebunny/crossref_metadata_2025 huggingface dataset:
Selective mode excitations and spontaneous emission engineering in quantum emitter-photonic structure coupled systems
Authors: Fang Wei , Lin Baoqing , Li Gao-xiang , Yang Yaping
Published: May 2022

We study the excitation conditions of the supported field modes, as well as the spontaneous decay property of a two-level quantum emitter coupled to photonic structures containing topological insulators (TIs) and left-handed materials. Within the proper field quantization scheme, the spontaneous decay rates of dipoles with different polarizations are expressed in forms of the Green’s functions. We find that in the proposed structure, the variation in the topological magnetoelectric polarizability (TMP) has a deterministic effect on the excitation of different field modes. As the result, the spontaneous decay property of the quantum emitter can be engineered. For a dipole placed in different spatial regions, the spontaneous decay feature indicates a dominant contribution from the waveguide modes, the surface plasmon modes or the free vacuum modes. Moreover, a special kind of the surface plasmon modes displaying asymmetric density of states at the interfaces, becomes legal in the presence of nontrivial TIs. These phenomena manifest the feasibility in controlling dipole emissions via manipulations of the topological magnetoelectric (TME) effect. Our results have potential applications in quantum technologies relied on the accurate control over light-matter interactions.
I-line photolithographic metalenses enabled by distributed optical proximity correction with a deep-learning model
Authors: Liao Wei-Ping , Liu Hsueh-Li , Lin Yu-Fan , Su Sheng-Siang , Chen Yu-Teng , Lin Guan-Bo , Tseng Tsung-Chieh , Lin Tong-Ke , Chen Chun-Chi , Huang Wen-Hsien , Chen Shih-Wei , Shieh Jia-Min , Yu Peichen , Chang You-Chia
Published: May 2022

High pattern fidelity is paramount to the performance of metalenses and metasurfaces, but is difficult to achieve using economic photolithography technologies due to low resolutions and limited process windows of diverse subwavelength structures. These hurdles can be overcome by photomask sizing or reshaping, also known as optical proximity correction (OPC). However, the lithographic simulators critical to model-based OPC require precise calibration and have not yet been specifically developed for metasurface patterning. Here, we demonstrate an accurate lithographic model based on Hopkin’s image formulation and fully convolutional networks (FCN) to control the critical dimension (CD) patterning of a near-infrared (NIR) metalens through a distributed OPC flow using i-line photolithography. The lithographic model achieves an average ΔCD/CD = 1.69% due to process variations. The model-based OPC successfully produces the 260 nm CD in a metalens layout, which corresponds to a lithographic constant k1 of 0.46 and is primarily limited by the resolution of the photoresist. Consequently, our fabricated NIR metalens with a diameter of 1.5 mm and numerical aperture (NA) of 0.45 achieves a measured focusing efficiency of 64%, which is close to the calculated value of 69% and among the highest reported values using i-line photolithography.
Bidirectional tandem-pumped high-brightness 6 kW level narrow-linewidth confined-doped fiber amplifier exploiting the side-coupled technique
Authors: Wu Hanshuo , Song Jiaxin , Ma Pengfei , Liu Wei , Ren Shuai , Wang Guangjian , Li Ruixian , Xiao Hu , Huang Liangjin , Leng Jinyong , Pan Zhiyong , Zhou Pu
Published: May 2022

In this work, a bidirectional tandem-pumped high-power narrow-linewidth confined-doped ytterbium fiber amplifier is demonstrated based on side-coupled combiners. Benefiting from the large-mode-area design of the confined-doped fiber, the nonlinear effects, including stimulated Raman (SRS) and stimulated Brillouin scattering (SBS), are effectively suppressed. While the transverse mode instability (TMI) effect is also mitigated through the combination of confined-doped fiber design and the bidirectional tandem pumping scheme. As a result, narrow-linewidth fiber laser with 5.96 kW output power is obtained, the slope efficiency and the 3-dB linewidth of which are ∼81.7% and 0.42 nm, respectively. The beam quality is well maintained during the power scaling process, being around M2 = 1.6 before the TMI occurs, and is well kept (M2 = 2.0 at 5.96 kW) even after the onset of TMI. No SRS or SBS is observed at the maximum output power, and the signal-to-noise ratio reaches as high as ∼61.4 dB. To the best of our knowledge, this is the record power ever reported in narrow-linewidth fiber lasers. This work could provide a good reference for realizing high-power high-brightness narrow-linewidth fiber lasers.
Transparent dual-band ultraviolet photodetector based on graphene/p-GaN/AlGaN heterojunction
Authors: Wu Gang , Tang Libin , Deng Gongrong , Liu Lining , Hao Qun , Yuan Shouzhang , Wang Jingyu , Wei Hong , Zhao Yupeng , Yue Biao , Shi Jingmei , Tan Ying , Li Rujie , Zhang Yiyun , Yan Jianchang , Yi Xiaoyan , Wang Junxi , Kong Jincheng , Li Jinmin
Published: May 2022

Versatile applications have driven a desire for dual-band detection that enables seeing objects in multiple wavebands through a single photodetector. In this paper, a concept of using graphene/p-GaN Schottky heterojunction on top of a regular AlGaN-based p-i-n mesa photodiode is reported for achieving solar-/visible-blind dual-band (275 nm and 365 nm) ultraviolet photodetector with high performance. The highly transparent graphene in the front side and the polished sapphire substrate at the back side allows both top illumination and back illumination for the dual band detection. A system limit dark current of 1×10−9 A/cm2 at a negative bias voltage up to -10 V has been achieved, while the maximum detectivity obtained from the detection wavebands of interests at 275 nm and 365 nm are ∼ 9.0 ×1012 cm·Hz1/2/W at -7.5 V and ∼8.0 × 1011 cm·Hz1/2/W at +10 V, respectively. Interestingly, this new type of photodetector is dual-functional, capable of working as either photodiode or photoconductor, when switched by simply adjusting the regimes of bias voltage applied on the devices. By selecting proper bias, the device operation mode would switch between a high-speed photodiode and a high-gain photoconductor. The device exhibits a minimum rise time of ∼210 µs when working as a photodiode and a maximum responsivity of 300 A/W at 6 μW/cm2 when working as a photoconductor. This dual band and multi-functional design would greatly extend the utility of detectors based on nitrides.
Controllable high-speed rotated femtosecond cylindrical vector beam based on optical heterodyne interference
Authors: Zhao Yuwei , Fan Jintao , Song Youjian , Hu Minglie
Published: May 2022

Structured light beams that possess unique polarization distribution could offer a new degree of freedom for a variety of applications, and hence its flexible polarization manipulation is necessary. Here we experimentally report a heterodyne interference-based method for generating femtosecond cylindrical vector beam (CVB) with high-speed controllable rotated polarization states. The femtosecond CVBs are created through the superposition of two optical vortices with opposite handedness. The use of two acoustic-optical modulators (AOMs) with frequency differences allows to achieve polarization rotation in a hopping-free scheme at on demand speed. Up to 1 MHz of the rotation frequency is demonstrated by visualizing the fast rotation events through a fast-frame-rate CCD camera. Moreover, we show our method can be readily extended to produce higher order CVBs with more complex rotated polarization distributions. Such a simple yet versatile femtosecond polarization-controlled laser system has the capability to act as a nonlinear trapping platform, thus opening tremendous potential opportunities in the fields of micromachining, nanofabrication, and so force.
Development and characteristics of infrared gradient refractive index chalcogenide glasses by hot pressing
Authors: Gui Yiming , Guan Yongnian , Zhang Xusheng , Zhang Chunqiu , Xia Kelun , He Lelu , Shen Xiang , Liu Zijun
Published: May 2022

Compared with ordinary uniform lenses, the length and refractive index distribution of gradient refractive index (GRIN) lenses can effectively correct aberration and chromatic aberration. This advantage makes the miniaturization, integration, and lens lightweight possible. Although the visible GRIN lenses based on silicate glass are widely used, the infrared GRIN lenses based on chalcogenide glass are still elusive. This paper introduces a new method for preparing this kind of lens by hot pressing sintering diffusion of chalcogenide glasses. A series of chalcogenide glasses Ge10As22Se68-xSx (x = 4, 7, 10, 14, 24, 28, 34 mol%) with refractive index range from 2.37 to 2.57 (n@8 µm) and similar glass transition temperature (ΔTg < 10℃) were prepared by melt quenching. The relationship between Raman peaks and the refractive index of glasses was studied. Furthermore, the refractive index profile formed by elemental diffusion was characterized by Raman signals. The results show that the diffusion length reaches more than 290 µm, and larger diffusion distances can be achieved by stacking multiple layers. The obtained GRIN glass maintains good transmittance in the whole atmospheric window of 2 ∼ 12 µm.
In situ probing and stabilizing the power ratio of electro-optic-modulated laser pairs based on VIPA etalon for quantum sensing
Authors: Wang Guochao , Yang Mingyue , Wang Enlong , Zhang Xu , Jia Aiai , Zhu Lingxiao , Yan Shuhua , Yang Jun
Published: May 2022

Monitoring and stabilizing the power ratio of laser pairs is significant for high-precision atom interferometers, especially as the compact electro-optic-modulated all-fiber laser system prevails. In this Letter, we demonstrate a novel, to the best of our knowledge, method to in situ probe the relative power of laser pairs and to stabilize the power ratio of two Raman lasers using a high-dispersion virtually imaged phased array (VIPA) etalon. Sub-microsecond resolution on probing laser power transformation during the atom interferometer sequence is achieved and the power ratio of two Raman lasers (PRTR) is tightly locked with high bandwidth despite environmental disturbances, showing an Allan deviation of 4.39 × 10−5 at 1000 s averaging time. This method provides a novel way to stabilize the PRTR and diagnose multi-frequency laser systems for atom interferometers, and it could find potential applications in broad quantum sensing scenarios.
Electrically injected supersymmetric semiconductor lasers with narrow vertical divergence angle
Authors: Fu Ting , Qi Aiyi , Chen Jingxuan , Wang Yufei , Zhou Xuyan , Wang Xueyou , Dai Yingqiu , Wang Mingjin , Zheng Wanhua
Published: May 2022

Electrically injected supersymmetric (SUSY) semiconductor lasers are proposed and fabricated. Two successive SUSY transformations are applied to the main array arranged along the direction of epitaxial growth, which can remove the propagation constants of the fundamental mode and the leaky mode of the main array from the superpartner while keeping those of other high-order modes. The SUSY laser possesses an excellent mode discrimination and favors the lasing of the fundamental mode. The fabricated SUSY laser can emit light with a single-lobe vertical far-field pattern with the full width at half maximum of 16.87° under an injection current of 1.4 A.
Stealth dicing of 1-mm-thick glass with aberration-free axial multi-focus beams
Authors: Zhang Huaizhi , Xu Jiaming , Li Hengyang , Xiao Yu , Cheng Wei , Tang Xiahui , Qin Yingxiong
Published: May 2022

Laser stealth dicing can realize material separation with negligible surface damage, but severe aberrations in thick materials degrade processing quality. This Letter presents a nonlinear point-to-point transformation method combined with spherical aberration compensation to achieve aberration-free axial multi-focus beams. The focus peak intensity increases 7 times at a depth of 0.5 mm after spherical aberration compensation, and reaches 44 times at 3.5 mm. Spherical aberration compensation experiments showed that the width of the heat-affected zone remains almost unchanged at different depths inside the glass, and stealth dicing experiments for 1-mm-thick glass demonstrated that aberration-free 1-focus, 2-foci, and 3-foci stealth dicing can be successfully realized.
No evidence of fetal defects or anti-syncytin-1 antibody induction following COVID-19 mRNA vaccination
Authors: Lu-Culligan Alice , Tabachnikova Alexandra , Pérez-Then Eddy , Tokuyama Maria , Lee Hannah J. , Lucas Carolina , Silva Monteiro Valter , Miric Marija , Brache Vivian , Cochon Leila , Muenker M. Catherine , Mohanty Subhasis , Huang Jiefang , Kang Insoo , Dela Cruz Charles , Farhadian Shelli , Campbell Melissa , Yildirim Inci , Shaw Albert C. , Ma Shuangge , Vermund Sten H. , Ko Albert I. , Omer Saad B. , Iwasaki Akiko
Published: May 2022

The impact of Coronavirus Disease 2019 (COVID-19) mRNA vaccination on pregnancy and fertility has become a major topic of public interest. We investigated 2 of the most widely propagated claims to determine (1) whether COVID-19 mRNA vaccination of mice during early pregnancy is associated with an increased incidence of birth defects or growth abnormalities; and (2) whether COVID-19 mRNA-vaccinated human volunteers exhibit elevated levels of antibodies to the human placental protein syncytin-1. Using a mouse model, we found that intramuscular COVID-19 mRNA vaccination during early pregnancy at gestational age E7.5 did not lead to differences in fetal size by crown-rump length or weight at term, nor did we observe any gross birth defects. In contrast, injection of the TLR3 agonist and double-stranded RNA mimic polyinosinic-polycytidylic acid, or poly(I:C), impacted growth in utero leading to reduced fetal size. No overt maternal illness following either vaccination or poly(I:C) exposure was observed. We also found that term fetuses from these murine pregnancies vaccinated prior to the formation of the definitive placenta exhibit high circulating levels of anti-spike and anti-receptor-binding domain (anti-RBD) antibodies to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) consistent with maternal antibody status, indicating transplacental transfer in the later stages of pregnancy after early immunization. Finally, we did not detect increased levels of circulating anti-syncytin-1 antibodies in a cohort of COVID-19 vaccinated adults compared to unvaccinated adults by ELISA. Our findings contradict popular claims associating COVID-19 mRNA vaccination with infertility and adverse neonatal outcomes.
Multilevel analysis of continuation of maternal healthcare services utilization and its associated factors in Ethiopia: A cross-sectional study
Authors: Chaka Eshetu E.
Published: May 2022

Continuum of care (CoC) has been recognized as a crucial strategy for minimizing maternal, neonatal, and child mortality. CoC promotes integrated Maternal Neonatal and Child Health (MNCH) services by linking together three aspects of maternal health care antenatal care, skilled birth attendance, and postnatal care. The study aimed to assess continuation of maternal healthcare services utilization and its associated factors among reproductive age women at pregnancy, delivery and postnatal stages in Ethiopia. Cross-sectional study design conducted using Ethiopian 2016 Demographic and Health Survey data. All women with the most recent live birth in the last five years preceding the 2016 survey were the study population. The sample size was 7590, 2415, and 1342 at service entry (ANC use), COC at a delivery level, and CoC at Postpartum level respectively. COC was measured at three levels of maternal health care (during pregnancy, delivery, and postpartum). The CoC is constructed from four or more antenatal care visits (ANC4+), skilled birth attendance (SBA), and postnatal care (PNC). About 9.1% of women received all components of CoC. Educational attainment, wealth quintile, and media exposure were associated with four or more antenatal care visits and COC at the delivery level. Perception of getting money for healthcare, having blood pressure measured and urine sample taken during ANC was associated with continuity of care at the delivery level and continuity of care at a postpartum level. Birth order, residence, and region were common factors associated with each outcome of interest. The proportion of women who received all ANC4+, SBA, and PNC across the CoC was low in Ethiopia. Effort needed to increase CoC at each stage. The study shows that focusing on place of residence and regional state variation is necessary to improve CoC at each level. Thus, contextualizing the strategies and further research are critical.
Instruments used to measure knowledge and attitudes of healthcare professionals towards antibiotic use for the treatment of urinary tract infections: A systematic review
Authors: Mwape Angela Kabulo , Schmidtke Kelly Ann , Brown Celia
Published: May 2022

Background

Urinary tract infections (UTIs) are the second most common condition (after upper respiratory tract infections) for which adults receive antibiotics, and this prevalence may contribute to antibiotic resistance. Knowledge and attitudes have been identified as potential determinants of antibiotic prescribing behaviour among healthcare professionals in the treatment and management of UTIs. An instrument that captures prescribers’ baseline knowledge of and attitudes towards antibiotic prescribing for UTIs could inform interventions to enhance prescribing. The current systematic review evaluates the psychometric properties of instruments already available and describes the theoretical constructs they measure.

Methods

Five electronic databases were searched for published studies and instruments. The Consensus-based Standards for the selection of health status Measurement Instruments checklist was used to assess the psychometric quality reporting of the instruments. The items included in each instrument were mapped onto the theoretical constructs underlying knowledge and attitudes using a mixed-theoretical model developed for this study.

Results

Fourteen studies met the review inclusion criteria. All instruments were available for review. None of the instruments had all the psychometric properties evaluated. Most of the instruments sought to identify knowledge and/or attitude factors influencing antibiotic prescribing for UTIs rather than to measure/assess knowledge and attitudes.

Conclusions

Few instruments for the assessment of knowledge and attitudes of healthcare professionals towards antibiotic use and UTI treatment are available. None of the instruments underwent the full development process to ensure that all psychometric properties were met. Furthermore, none of the instruments assessed all domains of knowledge and attitudes. Therefore, the ability of the instruments to provide a robust measurement of knowledge and attitudes is doubtful. There is a need for an instrument that fully and accurately measures the constructs of knowledge and attitude of healthcare professionals in the treatment of UTIs.
Evaluation of pharmacokinetic interactions of amoxicillin with ranitidine in healthy human volunteers of Karachi, Pakistan
Authors: Perveen Shaheen , Gauhar Shahnaz , Yousuf Rabia Ismail , Ali Huma , Zafar Farya , Sheikh Anab Fatima
Published: May 2022

Polypharmacy may be considered as the customary practice to provide optimum care services to patients but inter resulted in augmented probability of multiple drug interaction. Keeping in view the importance of drug interaction possibility, this study was designed to evaluate the effect of ranitidine on pharmacokinetics of amoxicillin in the local population of Karachi, Pakistan. Amoxicillin and ranitidine are the most commonly prescribed drugs to treat duodenal ulcer caused byHelicobacter pylori. The current investigation was carried out as a single center, open label, two phase, single dose, randomized way in cross over manner to evaluate the potential of pharmacokinetic interaction among amoxicillin formulation and ranitidine in adult healthy male volunteers. Post dosing blood samples were collected at multiple time points that are 0.5, 1, 1.5, 2, 3, 4, 6, 8 and 10 hours after administering amoxicillin 250mg capsule with and without ranitidine. For estimation of amoxicillin concentration in plasma, an HPLC method was developed and validated. The solvent system consisted of 0.025M phosphate buffer: acetonitrile (94:6 v/v). C18 column was employed with a flow rate of 1.0 ml/minute and at 230nm. A linear pattern with a correlation coefficient of 0.999 in the concentration ranges of 25μg/mL to 0.097μg/mL for amoxicillin and 25μg/mL to 0.048μg/mL for ranitidine was observed. Amoxicillin retention time was about 8 minutes and ranitidine retention time was around 12 minutes. Amoxicillin levels were computed and the concentrations were applied to calculate the pharmacokinetic parameters. Pharmacokinetic parameters were estimated by Kinetica TM 4.4.1 (Thermo Electron Corp. USA). The analysis of variance (two way) and t test (two one sided) were applied on log transformed pharmacokinetic parameters of amoxicillin. The Tmaxwas determined between amoxicillin alone and amoxicillin with ranitidine by Friedman test. The 90% confidence interval values for Cmax(calc)(0.687–0.743) and Tmax(calc)(1.148–1.742) for amoxicillin with or without ranitidine were not found within the FDA acceptable limits of 0.8–1.25. Study demonstrated the significant reduction in peak plasma levels of amoxicillin in presence of ranitidine. It is advisable to administer both drugs with time interval to avoid such interactions and increases in the bactericidal efficacy of amoxicillin.
Deep phylogenetic-based clustering analysis uncovers new and shared mutations in SARS-CoV-2 variants as a result of directional and convergent evolution
Authors: Nunes Danilo Rosa , Braconi Carla Torres , Ludwig-Begall Louisa F. , Arns Clarice Weis , Durães-Carvalho Ricardo
Published: May 2022

Nearly two decades after the last epidemic caused by a severe acute respiratory syndrome coronavirus (SARS-CoV), newly emerged SARS-CoV-2 quickly spread in 2020 and precipitated an ongoing global public health crisis. Both the continuous accumulation of point mutations, owed to the naturally imposed genomic plasticity of SARS-CoV-2 evolutionary processes, as well as viral spread over time, allow this RNA virus to gain new genetic identities, spawn novel variants and enhance its potential for immune evasion. Here, through an in-depth phylogenetic clustering analysis of upwards of 200,000 whole-genome sequences, we reveal the presence of previously unreported and hitherto unidentified mutations and recombination breakpoints in Variants of Concern (VOC) and Variants of Interest (VOI) from Brazil, India (Beta, Eta and Kappa) and the USA (Beta, Eta and Lambda). Additionally, we identify sites with shared mutations under directional evolution in the SARS-CoV-2 Spike-encoding protein of VOC and VOI, tracing a heretofore-undescribed correlation with viral spread in South America, India and the USA. Our evidence-based analysis provides well-supported evidence of similar pathways of evolution for such mutations in all SARS-CoV-2 variants and sub-lineages. This raises two pivotal points: (i) the co-circulation of variants and sub-lineages in close evolutionary environments, which sheds light onto their trajectories into convergent and directional evolution, and (ii) a linear perspective into the prospective vaccine efficacy against different SARS-CoV-2 strains.
Spatial analysis and influencing factors of pulmonary tuberculosis among students in Nanning, during 2012–2018
Authors: Yang Dan-ling , Li Wen , Pan Meng-hua , Su Hai-xia , Li Yan-ning , Tang Meng-ying , Song Xiao-kun
Published: May 2022

Background

Economically underdeveloped areas in western China are hotspots of tuberculosis, especially among students. However, the related spatial and temporal patterns and influencing factors are still unclear and there are few studies to analyze the causes of pulmonary tuberculosis in students from the perspective of space.

Methods

We collected data regarding the reported incidence of pulmonary tuberculosis (PTB) among students at township level in Nanning, from 2012 to 2018. The reported incidence of pulmonary tuberculosis among students in Nanning was analyzed using spatial autocorrelation and spatial scan statistical analysis to depict hotspots of PTB incidence and spatial and temporal clustering. Spatial panel data of the reported incidence rates and influencing factors at district and county levels in Nanning were collected from 2015 to 2018. Then, we analyzed the spatial effects of incidence and influencing factors using the spatial Durbin model to explore the mechanism of each influencing factor in areas with high disease prevalence under spatial effects.

Results

From 2012 to 2018, 1609 cases of PTB were reported among students in Nanning, with an average annual reported incidence rate of 14.84/100,000. Through the Joinpoint regression model, We observed a steady trend in the percentage of cases reported each year (P>0.05). There was spatial autocorrelation between the annual reported incidence and the seven-years average reported incidence from 2012 to 2018. The high-incidence area was distributed in the junction of six urban areas and spread to the periphery, with the junction at the center. The population of college students, per capita financial expenditure on health, per capita gross domestic product, and the number of health technicians per 1,000 population were all influencing factors in the reported incidence of PTB among students.

Conclusion

We identified spatial clustering of the reported incidence of PTB among students in Nanning, mainly located in the urban center and its surrounding areas. The clustering gradually decreased from the urban center to the surrounding areas. Spatial effects influenced the reported incidence of PTB. The population density of college students, per capita health financial expenditure, gross domestic product (GDP) per capita, and the number of health technicians per 1,000 were all influencing factors in the reported incidence of PTB among students.
Impact of colonization with multidrug-resistant organisms on antibiotic prophylaxis in patients with cirrhosis and variceal bleeding
Authors: Mücke Victoria T. , Peiffer Kai-Henrik- , Kessel Johanna , Schwarzkopf Katharina M. , Bojunga Jörg , Zeuzem Stefan , Finkelmeier Fabian , Mücke Marcus M.
Published: May 2022

Background

The efficacy of antibiotic prophylaxis to prevent rebleeding or infection after variceal bleeding in patients with liver cirrhosis colonized with multidrug-resistant organisms (MDROs) is unknown.

Methods

In this retrospective study, patients with liver cirrhosis and endoscopically confirmed variceal bleeding who were treated at a tertiary care center in Germany and were screened for MDROs at the time of bleeding were eligible for inclusion. Efficacy of antibiotic prophylaxis was evaluated in patients stratified according to microbiological susceptibility testing.

Results

From 97 patients, the majority had decompensated liver cirrhosis (median MELD Score 17) and ACLF was present in half of the patients (47.4%). One third of patients were colonized with MDRO at baseline. De-novo infection until day 10 or the combination of de-novo infection or rebleeding were comparable among both groups (p = 0.696 and p = 0.928, log-rank-test). Risk of de-novo infection or rebleeding was not significantly increased in patients who received antibiotic prophylaxis that did not cover the MDRO found upon baseline screening. Acute-on-chronic liver failure at baseline was the strongest and only independent risk factor that was associated with both outcomes (OR 5.52, 95%-CI 1.48–20.61, p = 0.011 and OR 11.5, 95%-CI 2.70–48.62, p<0.001). Neither MDRO colonization at baseline nor covering all detected MDRO with antibiotic prophylaxis (i.e. “adequate” prophylaxis) impacted transplant-free survival. Again, the presence of ACLF was the strongest independent risk factor associated with mortality (OR 9.85, 95%-CI 3.58–27.12, p<0.0001).

Conclusion

In this study, MDRO colonization did not increase the risk of rebleeding, infections nor death, even if antibiotic prophylaxis administered did not cover all MDRO detected at MDRO screening. Patients with ACLF had an increased risk of bleeding, infections and death.
Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron
Authors: Teng I-Ting , Nazzari Alexandra F. , Choe Misook , Liu Tracy , Oliveira de Souza Matheus , Petrova Yuliya , Tsybovsky Yaroslav , Wang Shuishu , Zhang Baoshan , Artamonov Mykhaylo , Madan Bharat , Huang Aric , Lopez Acevedo Sheila N. , Pan Xiaoli , Ruckwardt Tracy J. , DeKosky Brandon J. , Mascola John R. , Misasi John , Sullivan Nancy J. , Zhou Tongqing , Kwong Peter D.
Published: May 2022

Since the outbreak of the COVID-19 pandemic, widespread infections have allowed SARS-CoV-2 to evolve in human, leading to the emergence of multiple circulating variants. Some of these variants show increased resistance to vaccine-elicited immunity, convalescent plasma, or monoclonal antibodies. In particular, mutations in the SARS-CoV-2 spike have drawn attention. To facilitate the isolation of neutralizing antibodies and the monitoring of vaccine effectiveness against these variants, we designed and produced biotin-labeled molecular probes of variant SARS-CoV-2 spikes and their subdomains, using a structure-based construct design that incorporated an N-terminal purification tag, a specific amino acid sequence for protease cleavage, the variant spike-based region of interest, and a C-terminal sequence targeted by biotin ligase. These probes could be produced by a single step using in-process biotinylation and purification. We characterized the physical properties and antigenicity of these probes, comprising the N-terminal domain (NTD), the receptor-binding domain (RBD), the RBD and subdomain 1 (RBD-SD1), and the prefusion-stabilized spike ectodomain (S2P) with sequences from SARS-CoV-2 variants of concern or of interest, including variants Alpha, Beta, Gamma, Epsilon, Iota, Kappa, Delta, Lambda, Mu, and Omicron. We functionally validated probes by using yeast expressing a panel of nine SARS-CoV-2 spike-binding antibodies and confirmed sorting capabilities of variant probes using yeast displaying libraries of plasma antibodies from COVID-19 convalescent donors. We deposited these constructs to Addgene to enable their dissemination. Overall, this study describes a matrix of SARS-CoV-2 variant molecular probes that allow for assessment of immune responses, identification of serum antibody specificity, and isolation and characterization of neutralizing antibodies.
Risk stratification for hospital-acquired venous thromboembolism in medical patients (RISE): Protocol for a prospective cohort study
Authors: Choffat Damien , Farhoumand Pauline Darbellay , Jaccard Evrim , de la Harpe Roxane , Kraege Vanessa , Benmachiche Malik , Gerber Christel , Leuzinger Salomé , Podmore Clara , Truong Minh Khoa , Dumans-Louis Céline , Marti Christophe , Reny Jean-Luc , Aujesky Drahomir , Rakovic Damiana , Limacher Andreas , Rossel Jean-Benoît , Baumgartner Christine , Méan Marie
Published: May 2022

Background

Hospital-acquired venous thromboembolism (VTE) is one of the leading preventable causes of in-hospital mortality. However, its risk assessment in medically ill inpatients is complicated due to the patients’ heterogeneity and complexity of currently available risk assessment models (RAMs). The simplified Geneva score provides simplicity but has not yet been prospectively validated. Immobility is an important predictor for VTE in RAMs, but its definition is inconsistent and based on subjective assessment by nurses or physicians. In this study, we aim to prospectively validate the simplified Geneva score and to examine the predictive performance of a novel and objective definition of in-hospital immobilization using accelerometry.

Methods and analysis

RISE is a multicenter prospective cohort study. The goal is to recruit 1350 adult inpatients admitted for medical illness in three Swiss tertiary care hospitals. We collect data on demographics, comorbidities, VTE risk and thromboprophylaxis. Mobility from admission to discharge is objectively measured using a wrist-worn accelerometer. Participants are followed for 90 days for the occurrence of symptomatic VTE (primary outcome). Secondary outcomes are the occurrence of clinically relevant bleeding, and mortality. The evolution of autonomy in the activities of daily living, the length of stay, and the occurrence of readmission are also recorded. Time-dependent area under the curve, sensitivity, specificity, and positive and negative predictive values are calculated for each RAM (i.e. the simplified and original Geneva score, Padua, and IMPROVE score) with and without the objective mobility measures to assess their accuracy in predicting hospital-acquired VTE at 90 days.

Ethics and expected impact

The ethics committee approved the protocol and the study was registered on ClinicalTrials.gov asNCT04439383. RISE has the potential to optimize VTE risk stratification, and thus to improve the quality of care of medically hospitalized patients.
Information about dissemination of trial results in patient information leaflets for clinicals trials in the UK and Ireland: The what and the when
Authors: Bjorklund Matilda , Shiely Frances , Gillies Katie
Published: May 2022

Introduction

Complete and understandable information is vital for informed consent and this includes how and when potential participants can expect to receive trial results. Informing participants during informed consent about the sharing of trial results is important for addressing participants’ needs, ensuring adherence to regulatory guidance, and in fulfilling a moral obligation.

Methods

Patient Information Leaflets (PILs) were collated from across the UK and Ireland. Trial characteristics and data on disseminating trial results was extracted. Analysis included descriptive statistics and a directed content analysis approach. The content analysis framework was informed by regulatory guidance on PIL content and existing research on dissemination of trial results. Results were analysed using descriptive statistics and presented as a narrative summary as appropriate.

Results

238 PILs from 178 trials were analysed. Of the 238 PILs, 74% (n = 176) provided information on sharing results with participants, 70% (n = 123) of which described passive methods of disseminating results that require active engagement from the trial participants, i.e., effort required by the participant to seek the results. The majority (90%) of PILs included more than one proposed mode of dissemination that largely targeted healthcare professionals rather than participants. Only 8% of PILs specified a time period for when results could be expected, 47% did not specify a time period (e.g. at end of trial), and 45% included no information on when trial results would be available.

Conclusion

This study found that majority of the PILs included did include some information about dissemination of trial results. However, modes of dissemination tended to target researchers and clinicians rather than participants and information on when results would be available was often lacking. The findings highlight the need for further research that includes stakeholder input to identify what information on results summaries participants need at the point of making a decision about trial participation.
Payments and freedoms: Effects of monetary and legal incentives on COVID-19 vaccination intentions in Germany
Authors: Sprengholz Philipp , Henkel Luca , Betsch Cornelia
Published: May 2022

Monetary and legal incentives have been proposed to promote COVID-19 vaccination uptake. To evaluate the suitability of incentives, an experiment with German participants examined the effects of payments (varied within subjects: 0 to 10,000 EUR) and freedoms (varied between subjects: vaccination leading vs. not leading to the same benefits as a negative test result) on the vaccination intentions of previously unvaccinated individuals (n = 782) in April 2021. While no effect could be found for freedoms, the share of participants willing to be vaccinated increased with the payment amount. However, a significant change required large rewards of 3,250 EUR or more. While monetary incentives could increase vaccination uptake by a few percentage points, the high costs of implementation challenge the efficiency of the measure and call for alternatives. As the data suggest that considering vaccination as safe, necessary, and prosocial increases an individual’s likelihood of wanting to get vaccinated without payment, interventions should focus on these features when promoting vaccination against COVID-19.
Profil és profilalkotás a digitális életvilágban
Authors: Jakab György , Patai Rita
Published: May 2022

Mivel a magyar pedagógiai köztudatban a digitális életvilág és digitális profilalkotás fogalma még nem igazán elterjedt, ezért itt és most röviden vázoljuk ennek kereteit és főbb sajátosságait. Az írás – témánknak megfelelően – a digitális életvilág kockázataival, valamint a profilalkotás különböző módjaival és felhasználási területeivel foglalkozik.
The Ability of Lipoprotein (a) Level to Predict Early Carotid Atherosclerosis Is Impaired in Patients With Advanced Liver Fibrosis Related to Metabolic-Associated Fatty Liver Disease
Authors: Wu Tingfeng , Ye Junzhao , Shao Congxiang , Lin Yansong , Wang Wei , Feng Shiting , Zhong Bihui
Published: May 2022

INTRODUCTION:

Hepatic fibrosis reduces the serum level of lipoprotein (a) (Lp(a)) and may affect its accuracy in cardiovascular disease prediction of metabolic-associated fatty liver disease (MAFLD). We aimed to estimate the association between Lp(a) levels and the risk of carotid atherosclerosis in MAFLD patients with advanced fibrosis.

METHODS:

This was a cross-sectional study enrolling 4,348 consecutive individuals (1,346 patients with MAFLD and 3,002 non-MAFLD patients) who were admitted to the First Affiliated Hospital, Sun Yat-sen University, and underwent abdominal and carotid ultrasonography from 2015 to 2021. Lp(a) levels, liver biochemical markers, metabolic indices, and anthropometric parameters were measured. Liver fat content and fibrosis severity were assessed by MRI-PDFF, using the NAFLD fibrosis score (NFS) and liver stiffness measurement (LSM) of two-dimensional shear wave elastography, respectively.

RESULTS:

There was an L-shaped relationship between Lp(a) levels and LSMs in patients with MAFLD, and Lp(a) levels had a different relationship with liver fat content in MAFLD patients with F1-2 versus those with F3-4. Non-MAFLD patients had higher levels of Lp(a) than MAFLD patients with or without advanced fibrosis (both P < 0.05). Lp(a) levels and degree of liver fibrosis were both positively correlated with carotid atherosclerosis in patients with MAFLD. Lp(a) levels performed well on carotid atherosclerosis risk prediction for non-MAFLD patients with an area under the curve (AUC) of 0.819, which was significantly better than the carotid atherosclerosis risk prediction for MAFLD patients with NFS ≤ −1.836 (AUC: 0.781), NFS > −1.836 (AUC: 0.692), and LSM ≥ 9.0 kPa (AUC: 0.635) (all P < 0.05).

DISCUSSION:

Advanced liver fibrosis significantly reduces the predictive value of Lp(a) levels for the risk of carotid atherosclerosis in patients with MAFLD.
An unusual presentation of DiGeorge syndrome
Authors: Garkaby Jenny , Abrego Fuentes Laura Edith , Willett Pachul Jessica , Watts-Dickens Abby , Fraser Meghan
Published: May 2022

Introduction: DiGeorge syndrome is a heterogenous disorder with various clinical presentations. Common features include thymic hypoplasia, T cell lymphopenia, conotruncal heart defects, facial dysmorphism, cleft palate, developmental delay, and hypoparathyroidism. The severity of this condition varies, however typical presentation includes congenital heart defects and characteristic facial features. Isolated hypocalcemia in DiGeorge syndrome is rarely seen in neonates but rather as the sole manifestation in older teenagers or adults.

Aim: To report a case of an atypical presentation of DiGeorge syndrome.

Results: We report here a case of an infant who was diagnosed with DiGeorge syndrome, with seizures being the only clinical manifestation displayed by the patient. He was found to have low T cell receptor excision circle levels on a newborn screen (NBS) for severe combined immunodeficiency (SCID). He did not have facial dysmorphism nor cardiac defect.

Conclusion: Our case shows that severe hypocalcemia can be the only presenting symptom in DiGeorge syndrome. Based on this case, we recommend physicians test for calcium levels and PTH at the first encounter with a patient who screened positive during NBS for SCID.

Statement of Novelty: We describe an infant with DiGeorge syndrome who presented with severe hypocalcemia.
Internal quality control – past, present and future trends
Authors: Ricós Carmen , Fernandez-Calle Pilar , Perich Carmen , Westgard James O.
Published: May 2022

Objectives

This paper offers an historical view, through a summary of the internal quality control (IQC) models used from second half of twentyth century to those performed today and wants to give a projection on how the future should be addressed.

Methods

The material used in this work study are all papers collected referring IQC procedures. The method used is the critical analysis of the different IQC models with a discussion on the weak and the strong points of each model.

Results

First models were based on testing control materials and using multiples of the analytical procedure standard deviation as control limits. Later, these limits were substituted by values related with the intended use of test, mainly derived from biological variation. For measurands with no available control material methods based on replicate analysis of patient’ samples were developed and have been improved recently; also, the sigma metrics that relates the quality desired with the laboratory performance has resulted in a highly efficient quality control model. Present tendency is to modulate IQC considering the workload and the impact of analytical failure in the patent harm.

Conclusions

This paper remarks the strong points of IQC models, indicates the weak points that should be eliminated from practice and gives a future projection on how to promote patient safety through laboratory examinations.
Age and sex specific reference intervals of 13 hematological analytes in Chinese children and adolescents aged from 28 days up to 20 years: the PRINCE study
Authors: Song Wenqi , Yan Ruohua , Peng Mingting , Jiang Hong , Li Guixia , Cao Sancheng , Jiang Yongmei , Guo Zhenxin , Chen Dapeng , Yang Hongling , Xu Jin , Chang Yong , Xiang Yun , Zhao Min , Li Chenbin , Shen Ying , Jin Fang , Li Qiliang , Wang Yan , Peng Yaguang , Hu Lixin , Liu Ying , Zhang Xiaofei , Chen Wenxiang , Peng Xiaoxia , Ni Xin
Published: May 2022

Objectives

Pediatric Reference Intervals in China (PRINCE) is a nationwide initiative that aims to establish and validate harmonized reference intervals (RIs) for Chinese children and adolescents, in which 15,150 healthy volunteers aged up to 20 years were recruited from 11 centers to establish RIs and 7,557 children and adolescents were enrolled from 21 centers to validate RIs.

Methods

The complete blood cell counts (CBC) of venous whole blood were measured by hematology analyzers through Sysmex systems in different centers. Age- and sex-specific RIs were calculated according to the guidelines.

Results

Unlike adults with certain levels of analyte concentrations, hematological parameters of children changed through growth and development. Red blood cell counts, hemoglobin, and hematocrit increased with age, and revealed higher concentrations in boys than girls after puberty. White blood cell counts and platelet counts showed significant higher levels than adults before 2 years of age, and then gradually decreased without distinct sex differences. In addition, lymphocyte counts decreased with age while neutrophil counts showed an opposite trend. The lower and upper limits of pediatric RIs of CBC were different from those of adults.

Conclusions

The validation of RIs indicated that the PRINCE study provided a version of RIs suitable for most of regions in China. This first harmonized pediatric RIs of CBC across China provided a robust database to understand the dynamic changes of hematologic parameters from birth to adolescence, and will contribute to clinical diagnosis and prognosis evaluation for pediatric patients as well.
Convergence of generalized sampling series in weighted spaces
Authors: Acar Tuncer , Alagöz Osman , Aral Ali , Costarelli Danilo , Turgay Metin , Vinti Gianluca
Published: May 2022

The present paper deals with an extension of approximation properties of generalized sampling series to weighted spaces of functions. A pointwise and uniform convergence theorem for the series is proved for functions belonging to weighted spaces. A rate of convergence by means of weighted moduli of continuity is presented and a quantitative Voronovskaja-type theorem is obtained.
Verschwörungstheorien und das Erbe der Aufklärung: Auf den Schultern von Scheinriesen
Authors: Spiegel Thomas J.
Published: May 2022

Conspiracy theories are currently all the rage in philosophy and broader intellectual culture. One of the most common background assumptions in the discourse on conspiracy theories is that conspiracy theorists exhibit certain epistemic vices in the sense of cognitive misconduct. This epistemic vice is mostly seen as a form of irrationality; the corresponding “remedy”, as suggested by some commentators, is a return to the ideals of the Enlightenment. This article argues that this idea is wrongheaded. Upon closer inspection, it becomes clear that conspiracy theorists are actually motivated by the rational Enlightenment ideal of self-thinking in the first place. In contrast to the standard discourse, the article posits that conspiracism is based on a certain form of social scepticism, according to which conspiracy theorists radically mistrust a certain form of expert testimony, namely “official” statements. This form of social scepticism in turn facilitates a naive appropriation of the Enlightenment ideal of self-thinking. The article closes by drawing connections to the ethical and epistemological debate on trust and offers the pessimistic assessment that there are no easy solutions based on individual epistemic virtues.