bluuebunny/crossref_metadata_2025 huggingface dataset:
Does Anti-TNF-α Therapy Affect the Bacteriological Profile of Specimens Collected from Perianal Lesions? A Retrospective Analysis in Patients with Crohn’s Disease
Authors: Gruszecka Jolanta , Filip Rafał
Published: March 2022

Anal abscesses and fistulas are potential complications of Crohn’s disease (CD). Chronic immunosuppression, loose stools, and poor wound healing in this population present challenges to the management of perianal diseases. The purpose of the study was to determine the predominant bacterial species found in specimens from perianal lesions causing anal abscess and/or fistula drainage in hospitalized patients, and to compare the number and type of microorganisms isolated from samples taken from patients undergoing biological therapy or traditionally treated. The outcomes of studies of patients treated for anal abscesses or fistulas from 2017 to 2019 were evaluated. Data obtained from medical records included culture and antibiotic sensitivity results of swabs from perianal lesions of isolated microorganisms. A total of 373 swabs were collected from perianal lesions during the analysis period, including 51 (49 positive samples) from patients with CD. The predominant pathogen was Escherichia coli (55%, p < 0.001), the second most common microorganism was Staphylococcus aureus (14.3%, p < 0.001). In vitro susceptibility testing showed E. coli, ESBL (strain with Extended Spectrum Beta-Lactamase) in five cases, S. aureus, MRSA (methicillin-resistant S. aureus -resistant to all beta-lactam antibiotics: penicillins with inhibitors, cephalosporins, monobactams, carbapenems, except for ceftaroline) in one sample. Biologic therapy does not affect the type of microorganisms isolated from perianal abscesses in patients with CD.
Decoupling between Economic Development and Carbon Emissions and Its Driving Factors: Evidence from China
Authors: Zhao Xiaochun , Jiang Mei , Zhang Wei
Published: March 2022

Analyzing the relationship between economic development and carbon emissions is conducive to better energy saving and emission reduction. This study is based on the panel data of China’s carbon emissions, from 2009 to 2019, and quantitative analysis of the relationship between carbon emissions and economic development through the Tapio decoupling model and the Logarithmic Mean Divisia Index (LMDI) decomposition model. The results show that: First, carbon emission and economic development are increasing year by year, and the development trend of economic growth rate and carbon emission growth rate presents the characteristics of consistency and stage. Second, China’s carbon emissions and economic development are basically in a weak decoupling state, and carbon emissions and economic development are positively correlated. Third, there are significant differences in decoupling indices among the four regions, mainly in that the central region is better than the eastern region, the eastern region is better than the northeast region, the northeast region is better than the western region, and the development of provinces in the region is unbalanced. Fourth, from the perspective of driving factors, the elasticity of population size and economic intensity can restrain the decoupling of carbon emissions, while the elasticity of energy intensity and carbon intensity have a positive effect. Finally, according to the results of empirical analysis, this paper focuses on promoting China’s emission reduction and energy sustainable development from the aspects of developing low-carbon and zero carbon technology, supporting new energy industries and promoting the construction of a carbon emission trading market.
Adolescents’ Long-Term Experiences of Manageability, Comprehensibility, and Meaningfulness of a Group-Based Exercise Intervention for Depression
Authors: Reinodt Sara , Haglund Emma , Bremander Ann , Jarbin Håkan , Larsson Ingrid
Published: March 2022

Physical exercise is a potentially effective treatment for adolescents with mild to moderate depression. However, there is a lack of long-term follow-ups to reveal adolescents’ experiences of exercise as a treatment for depression. The salutogenic concept of sense of coherence (SOC), comprising the domains manageability, comprehensibility, and meaningfulness is important to understand behaviour change. This study aimed to describe adolescents’ long-term experiences of manageability, comprehensibility, and meaningfulness of a group-based exercise intervention for depression. Fourteen adolescents with persistent depression were recruited from a psychiatric outpatient clinic and interviewed one year after participating in a 14-week moderate to vigorous exercise intervention for depression. An abductive qualitative content analysis was conducted, based on the three SOC domains manageability, comprehensibility, and meaningfulness. The results revealed that participation in the intervention was made manageable by a supportive environment, including: the intervention design, togetherness with peer group, and encouragement from adults. The comprehensibility of the intervention emerged through the insights regarding health benefits of exercise and the aim of the intervention. Meaningfulness was achieved through improved health behaviour, well-being and self-esteem, along with strengthened belief in the future and increased commitment to everyday life. The group-based exercise intervention was experienced as manageable, comprehensible, and meaningful.
Assessing Trade-Offs and Optimal Ranges of Density for Life Expectancy and 12 Causes of Mortality in Metro Vancouver, Canada, 1990–2016
Authors: Yu Jessica , Gustafson Paul , Tran Martino , Brauer Michael
Published: March 2022

Background: Understanding and managing the impacts of population growth and densification are important steps for sustainable development. This study sought to evaluate the health trade-offs associated with increasing densification and to identify the optimal balance of neighbourhood densification for health. Methods: We linked population density with a 27-year mortality dataset in Metro Vancouver that includes census-tract levels of life expectancy (LE), cause-specific mortalities, and area-level deprivation. We applied two methods: (1) difference-in-differences (DID) models to study the impacts of densification changes from the early 1990s on changes in mortality over a 27-year period; and (2) smoothed cubic splines to identify thresholds of densification at which mortality rates accelerated. Results: At densities above ~9400 persons per km2, LE began to decrease more rapidly. By cause, densification was linked to decreased mortality for major causes of mortality in the region, such as cardiovascular diseases, neoplasms, and diabetes. Greater inequality with increasing density was observed for causes such as human immunodeficiency virus and acquired immunodeficiency syndrome (HIV/AIDS), sexually transmitted infections, and self-harm and interpersonal violence. Conclusions: Areas with higher population densities generally have lower rates of mortality from the major causes, but these environments are also associated with higher relative inequality from largely preventable causes of death.
Racial Disparities in Cigarette Smoking Behaviors and Differences Stratified by Metropolitan Area of Residence
Authors: Arauz Rony F. , Mayer Margaret , Reyes-Guzman Carolyn , Ryan Bríd M.
Published: March 2022

Background: Black cigarette smokers experience a disproportionate burden of non-small cell lung cancer (NSCLC) compared to other racial and ethnic groups, despite starting to smoke later in life, smoking less frequently, and smoking fewer cigarettes per day compared with White smokers. Research has shown that these disparities in NSCLC are wider in rural areas. Objective: To examine differences in smoking behaviors between Black and White individuals living in non-metropolitan areas and metropolitan areas. Methods: Using harmonized data from the Tobacco Use Supplement to the Current Population Survey (TUS-CPS) years 2010–2011, 2014–2015, and 2018–2019, we compared smoking behaviors between Black and White current and former smokers by metropolitan status (i.e., whether an individual lives in a densely populated area or not) and by both metropolitan status and sex. Results: Smoking prevalence was higher among White participants living in non-metropolitan versus Black participants. Further, in non-metropolitan areas, Black individuals reported smoking fewer cigarettes per day, fewer years of smoking, and a later age of initiation compared to White individuals. Additionally, Black individuals, especially men, were more likely than White individuals to be current non-daily smokers. Conclusions: Our findings show that Black individuals living in non-metropolitan areas do not, in aggregate, have more cigarette smoking exposure relative to White individuals. Additional research is needed to further understand smoking-related exposures and other factors that may contribute to lung cancer disparities, especially in non-metropolitan areas.
Health-Related Quality of Life (HRQoL) of Residents with Persistent Lower Respiratory Symptoms or Asthma Following a Sulphur Stockpile Fire Incident
Authors: Adams Shahieda , Rajani Mayuri , Baatjies Roslynn , Omar Faieza , Jeebhay Mohamed Fareed
Published: March 2022

Background: This study evaluated health-related quality of life (HRQoL) in residents with persistent lower respiratory symptoms (PLRS) or asthma six years after exposure to sulphur dioxide vapours emanating from an ignited sulphur stockpile. Methods: A cross-sectional study was carried out, using interview data collected at three time points (prior to, one- and six-years post incident), medical history, respiratory symptoms and HRQOL using the Medical Outcomes Study Form 36 (SF-36). Results: A total of 246 records, 74 with and 172 without PLRS or asthma, were analysed. The mean age was 42 (SD:12) years in the symptomatic group and 41 (SD:13) years in the asymptomatic group. Mean SF-36 scores were significantly lower for the symptomatic group in the Physical Functioning (24 vs. 39), Role—Physical (33 vs. 48) and General Health (GH) domains (24 vs. 37). Symptomatic residents experienced a significant decline in their Role—Physical (OR = 1.97; CI 1.09, 3.55) and GH (OR = 3.50; CI 1.39, 8.79) at year 6 compared to asymptomatic participants. Residents with co-morbid reactive upper airways dysfunction syndrome demonstrated stronger associations for GH (OR = 7.04; CI 1.61, 30.7) at year 1 and at year 6 (OR = 8.58; CI 1.10, 65.02). Conclusions: This study highlights the long-term adverse impact on HRQoL among residents with PLRS or asthma following a sulphur stockpile fire disaster.
Predicting and Analyzing Road Traffic Injury Severity Using Boosting-Based Ensemble Learning Models with SHAPley Additive exPlanations
Authors: Dong Sheng , Khattak Afaq , Ullah Irfan , Zhou Jibiao , Hussain Arshad
Published: March 2022

Road traffic accidents are one of the world’s most serious problems, as they result in numerous fatalities and injuries, as well as economic losses each year. Assessing the factors that contribute to the severity of road traffic injuries has proven to be insightful. The findings may contribute to a better understanding of and potential mitigation of the risk of serious injuries associated with crashes. While ensemble learning approaches are capable of establishing complex and non-linear relationships between input risk variables and outcomes for the purpose of injury severity prediction and classification, most of them share a critical limitation: their “black-box” nature. To develop interpretable predictive models for road traffic injury severity, this paper proposes four boosting-based ensemble learning models, namely a novel Natural Gradient Boosting, Adaptive Gradient Boosting, Categorical Gradient Boosting, and Light Gradient Boosting Machine, and uses a recently developed SHapley Additive exPlanations analysis to rank the risk variables and explain the optimal model. Among four models, LightGBM achieved the highest classification accuracy (73.63%), precision (72.61%), and recall (70.09%), F1-scores (70.81%), and AUC (0.71) when tested on 2015–2019 Pakistan’s National Highway N-5 (Peshawar to Rahim Yar Khan Section) accident data. By incorporating the SHapley Additive exPlanations approach, we were able to interpret the model’s estimation results from both global and local perspectives. Following interpretation, it was determined that the Month_of_Year, Cause_of_Accident, Driver_Age and Collision_Type all played a significant role in the estimation process. According to the analysis, young drivers and pedestrians struck by a trailer have a higher risk of suffering fatal injuries. The combination of trailers and passenger vehicles, as well as driver at-fault, hitting pedestrians and rear-end collisions, significantly increases the risk of fatal injuries. This study suggests that combining LightGBM and SHAP has the potential to develop an interpretable model for predicting road traffic injury severity.
Effects of Small-Sided Games Training versus High-Intensity Interval Training Approaches in Young Basketball Players
Authors: Arslan Ersan , Kilit Bulent , Clemente Filipe Manuel , Murawska-Ciałowicz Eugenia , Soylu Yusuf , Sogut Mustafa , Akca Firat , Gokkaya Mine , Silva Ana Filipa
Published: March 2022

This study aimed to investigate the effects of the 6-week small-sided games training (SSGs) vs. high-intensity interval training (HIIT) on the psychophysiological and performance responses, and technical skills of young basketball players. Thirty-two male players (age: 14.5 ± 0.5 years of age) were randomly divided into SSGs group (n = 16) and HIIT group (n = 16) training methods thrice per week for 6 weeks. The players in the SSGs group performed two 5–8 min of 2 vs. 2 with 2 min rest periods, while the players in HIIT performed 12–18 min of runs at intensities (90 to 95%) related to the velocity obtained in the 30-15 intermittent fitness test (IFT). Pre-testing and post-testing sessions involved assessments of Yo-Yo Intermittent Recovery Test level 1, 30-15 intermittent fitness test, 5 and 30 m sprint times, vertical jump height, repeated sprint ability, defensive and offensive agility, and technical skills. The SSGs group demonstrated significantly higher agility-based technical responses in terms of the control dribbling and shooting skills (d = 1.71 vs. 0.20, d = 1.41 vs. 0.35, respectively) compared with the HIIT group. Conversely, the HIIT induced greater improvements in 30 m sprint times (d = 3.15 vs. 0.68). These findings provided that SSGs in youth basketball players may allow similar positive physical adaptations to HIIT, with an extra advantage of improving technical skills while improving enjoyability.
Nighttime Sleep Awakening Frequency and Its Consistency Predict Future Academic Performance in College Students
Authors: Ho Ghee Wee , Yang Zhenzhi , Xing Linna , Tsang Ken Kang-Too , Ruan Huada Daniel , Li Yu
Published: March 2022

Although the relationship between sleep and academic performance has been extensively examined, how sleep predicts future academic performance (e.g., 2–3 years) remains to be further investigated. Using wearable smartwatches and a self-report questionnaire, we tracked sleep activities of 45 college students over a period of approximately half a month to see whether their sleep activities predicted their academic performance, which was estimated by grade point average (GPA). Results showed that both nighttime sleep awakening frequency and its consistency in the tracking period were not significantly correlated with the GPA for the courses taken in the sleep tracking semester (current GPA). However, both nighttime sleep awakening frequency and its consistency inversely predicted the GPA for the rest of the courses taken after that semester (future GPA). Moreover, students with more difficulty staying awake throughout the day obtained lower current and future GPAs, and students with higher inconsistency of sleep quality obtained lower future GPA. Together, these findings highlight the importance of nighttime sleep awakening frequency and consistency in predicting future academic performance, and emphasize the necessity of assessing the consistency of sleep measures in future studies.
Using VBIM Technique to Discover ARMC4/ODAD2 as a Novel Negative Regulator of NF-κB and a New Tumor Suppressor in Colorectal Cancer
Authors: Martin Matthew , Mundade Rasika , Hartley Antja-Voy , Jiang Guanglong , Jin Jiamin , Sun Steven , Safa Ahmad , Sandusky George , Liu Yunlong , Lu Tao
Published: March 2022

Since nuclear factor (NF) κB plays pivotal roles in inflammation and cancer, understanding its regulation holds great promise for disease therapy. Using the powerful validation-based insertional mutagenesis (VBIM) technique established by us previously, we discovered armadillo repeat-containing protein 4 (ARMC4)/outer dynein arm docking complex subunit 2 (ODAD2), a rarely studied protein known to date, as a novel negative regulator of NF-κB in colorectal cancer (CRC). High expression of ARMC4 downregulated the expression of NF-κB-dependent genes, dramatically reduced NF-κB activity, cellular proliferation, anchorage-independent growth, and migratory ability in vitro, and significantly decreased xenograft tumor growth in vivo. Co-immunoprecipitation experiments demonstrated that ARMC4 forms a complex with NF-κB. Importantly, the lower ARMC4 expression in patient tumors than normal tissues indicates its potential tumor suppressor function in CRC. Collectively, we uncovered a completely new facet of ARMC4 function by identifying it as a novel NF-κB negative regulator, thus uncovering ARMC4 as a potential new therapeutic target in CRC.
Mitogen-Activated Protein Kinase and Substrate Identification in Plant Growth and Development
Authors: Jiang Min , Zhang Youze , Li Peng , Jian Jinjing , Zhao Changling , Wen Guosong
Published: March 2022

Mitogen-activated protein kinases (MAPKs) form tightly controlled signaling cascades that play essential roles in plant growth, development, and defense response. However, the molecular mechanisms underlying MAPK cascades are still very elusive, largely because of our poor understanding of how they relay the signals. The MAPK cascade is composed of MAPK, MAPKK, and MAPKKK. They transfer signals through the phosphorylation of MAPKKK, MAPKK, and MAPK in turn. MAPKs are organized into a complex network for efficient transmission of specific stimuli. This review summarizes the research progress in recent years on the classification and functions of MAPK cascades under various conditions in plants, especially the research status and general methods available for identifying MAPK substrates, and provides suggestions for future research directions.
Efficient Isolation and Functional Characterization of Niche Cells from Human Corneal Limbus
Authors: Polisetti Naresh , Sharaf Lyne , Schlötzer-Schrehardt Ursula , Schlunck Günther , Reinhard Thomas
Published: March 2022

The fate decision of limbal epithelial progenitor cells (LEPC) at the human corneal limbus is determined by the surrounding microenvironment with limbal niche cells (LNC) as one of its essential components. Research on freshly isolated LNC which mainly include limbal mesenchymal stromal cells (LMSC) and limbal melanocytes (LM) has been hampered by a lack of efficient protocols to isolate and purify these cells. We devised a protocol for rapid retrieval of pure LMSC, LM and LEPC populations by collagenase digestion of limbal tissue and subsequent fluorescence-activated cell sorting (FACS) using antibodies against CD90 and CD117. The sorted cells were characterized by immunophenotyping and functional assays. The effects of LMSC and LM on LEPC were studied in 3D co-cultures and LEPC differentiation status was assessed by immunohistochemistry. Enzymatic digestion and flow sorting yielded pure populations of LMSC (CD117−CD90+), LM (CD117+CD90−), and LEPC (CD117−CD90−). The LMSC exhibited self-renewal capacity (55.0 ± 4.6 population doublings), expressed mesenchymal stem cell markers (CD73, CD90, CD105, and CD44), and transdifferentiated to adipocytes, osteocytes, or chondrocytes. The LM exhibited self-renewal capacity and sustained melanin production. The sorted LEPC expressed epithelial progenitor markers (CK14, CK19, and CK15) and showed a colony-forming ability. Co-cultivation of LMSC and LM with LEPC resulted in a 4–5-layered stratified epithelium and supported the preservation of a LEPC phenotype, as reflected by increased p63+ and Ki67+ cells and decreased CK12+ cells compared with LEPC monocultures. A highly efficient isolation of pure LM, LMSC, and LEPC populations from a single preparation may allow for direct transcriptomic and proteomic profiling as well as functional studies on native unpassaged LNC, which can be considered as proper equivalents of LNC in vivo. The developed biomimetic 3D co-culture method could provide an experimental model for investigating the functional role of LNC in the limbal stem cell niche.
Changes in Metabotropic Glutamate Receptor Gene Expression in Rat Brain in a Lithium–Pilocarpine Model of Temporal Lobe Epilepsy
Authors: Kovalenko Anna A. , Zakharova Maria V. , Schwarz Alexander P. , Dyomina Alexandra V. , Zubareva Olga E. , Zaitsev Aleksey V.
Published: March 2022

Preventing epileptogenesis in people at risk is an unmet medical need. Metabotropic glutamate receptors (mGluRs) are promising targets for such therapy. However, drugs acting on mGluRs are not used in the clinic due to limited knowledge of the involvement of mGluRs in epileptogenesis. This study aimed to analyze the changes in gene expression of mGluR subtypes (1–5, 7, 8) in various rat brain regions in the latent and chronic phases of a lithium–pilocarpine model of epilepsy. For this study, multiplex test systems were selected and optimized to analyze mGluR gene expression using RT-qPCR. Region- and phase-specific changes in expression were revealed. During the latent phase, mGluR5 mRNA levels were increased in the dorsal and ventral hippocampus, and expression of group III genes was decreased in the hippocampus and temporal cortex, which could contribute to epileptogenesis. Most of the changes in expression detected in the latent stage were absent in the chronic stage, but mGluR8 mRNA production remained reduced in the hippocampus. Moreover, we found that gene expression of group II mGluRs was altered only in the chronic phase. The study deepened our understanding of the mechanisms of epileptogenesis and suggested that agonists of group III mGluRs are the most promising targets for preventing epilepsy.
Emerging Antifungal Targets and Strategies
Authors: Ivanov Marija , Ćirić Ana , Stojković Dejan
Published: March 2022

Despite abundant research in the field of antifungal drug discovery, fungal infections remain a significant healthcare burden. There is an emerging need for the development of novel antifungals since those currently available are limited and do not completely provide safe and secure protection. Since the current knowledge regarding the physiology of fungal cells and the infection mechanisms is greater than ever, we have the opportunity to use this for the development of novel generations of antifungals. In this review, we selected and summarized recent studies describing agents employing different antifungal mechanisms. These mechanisms include interference with fungal resistance, including impact on the efflux pumps and heat shock protein 90. Additionally, interference with virulence factors, such as biofilms and hyphae; the impact on fungal enzymes, metabolism, mitochondria, and cell wall; and antifungal vaccines are explored. The agents investigated belong to different classes of natural or synthetic molecules with significant attention given also to plant extracts. The efficacy of these antifungals has been studied mainly in vitro with some in vivo, and clinical studies are needed. Nevertheless, there is a large quantity of products employing novel antifungal mechanisms that can be further explored for the development of new generation of antifungals.
Synthesis and Solvent Dependent Fluorescence of Some Piperidine-Substituted Naphthalimide Derivatives and Consequences for Water Sensing
Authors: Tigoianu Radu , Airinei Anton , Georgescu Emilian , Nicolescu Alina , Georgescu Florentina , Isac Dragos Lucian , Deleanu Calin , Oancea Florin
Published: March 2022

Novel fluorescent strigolactone derivatives that contain the piperidine-substituted 1,8-naphthalimide ring system connected through an ether link to a bioactive 3-methyl-furan-2-one unit were synthesized and their spectroscopic properties investigated. The solvatochromic behavior of these piperidine-naphthalimides was monitored in solvents of different polarity using the electronic absorption and fluorescence spectra. These compounds exhibited a strong positive solvatochromism taking into account the change of solvent polarity, and the response mechanism was analyzed by fluorescence lifetime measurements. According to Catalan and [f(n), f(ε), β, α] solvent scales, the dipolarity and polarizability are relevant to describe the solute–solvent interactions. The emission chemosensing activity was discussed in order to determine the water content in organic environments. The emission intensity of these compounds decreased rapidly in dioxane, increasing water level up to 10%. Measuring of quantum yield indicated that the highest values of quantum efficiency were obtained in nonpolar solvents, while in polar solvents these derivatives revealed the lowest quantum yield. The fluorescence decay can be described by a monoexponential model for low water levels, and for higher water contents a biexponential model was valid.
The Molecular Basis of FIX Deficiency in Hemophilia B
Authors: Shen Guomin , Gao Meng , Cao Qing , Li Weikai
Published: March 2022

Coagulation factor IX (FIX) is a vitamin K dependent protein and its deficiency causes hemophilia B, an X-linked recessive bleeding disorder. More than 1000 mutations in the F9 gene have been identified in hemophilia B patients. Here, we systematically summarize the structural and functional characteristics of FIX and the pathogenic mechanisms of the mutations that have been identified to date. The mechanisms of FIX deficiency are diverse in these mutations. Deletions, insertions, duplications, and indels generally lead to severe hemophilia B. Those in the exon regions generate either frame shift or inframe mutations, and those in the introns usually cause aberrant splicing. Regarding point mutations, the bleeding phenotypes vary from severe to mild in hemophilia B patients. Generally speaking, point mutations in the F9 promoter region result in hemophilia B Leyden, and those in the introns cause aberrant splicing. Point mutations in the coding sequence can be missense, nonsense, or silent mutations. Nonsense mutations generate truncated FIX that usually loses function, causing severe hemophilia B. Silent mutations may lead to aberrant splicing or affect FIX translation. The mechanisms of missense mutation, however, have not been fully understood. They lead to FIX deficiency, often by affecting FIX’s translation, protein folding, protein stability, posttranslational modifications, activation to FIXa, or the ability to form functional Xase complex. Understanding the molecular mechanisms of FIX deficiency will provide significant insight for patient diagnosis and treatment.
Secondary Hyperparathyroidism in Children with Mucolipidosis Type II (I-Cell Disease): Irish Experience
Authors: Boruah Ritma , Monavari Ahmad Ardeshir , Conlon Tracey , Murphy Nuala , Stroiescu Andreea , Ryan Stephanie , Hughes Joanne , Knerr Ina , McDonnell Ciara , Crushell Ellen
Published: March 2022

Mucolipidosis type II (ML II) is an autosomal recessive lysosomal targeting disorder that may present with features of hyperparathyroidism. The aim of this study was to describe in detail the clinical cases of ML II presenting to a tertiary referral centre with biochemical and/or radiological features of hyperparathyroidism. There were twenty-three children diagnosed with ML II in the Republic of Ireland from July 1998 to July 2021 inclusive (a 23-year period). The approximate incidence of ML II in the Republic of Ireland is, therefore, 1 per 64,000 live births. Medical records were available and were reviewed for 21 of the 23 children. Five of these had been identified as having biochemical and/or radiological features of hyperparathyroidism. Of these five, three children were born to Irish Traveller parents and two to non-Traveller Irish parents. All five children had radiological features of hyperparathyroidism (on skeletal survey), with evidence of antenatal fractures in three cases and an acute fracture in one. Four children had biochemical features of secondary hyperparathyroidism. Three children received treatment with high dose Vitamin D supplements and two who had antenatal/acute fractures were managed with minimal handling. We observed resolution of secondary hyperparathyroidism in all cases irrespective of treatment. Four of five children with ML II and hyperparathyroidism died as a result of cardiorespiratory failure at ages ranging from 10 months to 7 years. Biochemical and/or radiological evidence of hyperparathyroidism is commonly identified at presentation of ML II. Further studies are needed to establish the pathophysiology and optimal management of hyperparathyroidism in this cohort. Recognition of this association may improve diagnostic accuracy and management, facilitate family counseling and is also important for natural history data.
Clinical Outcomes in Patients Aged 80 Years or Older Receiving Non-Invasive Respiratory Support for Hypoxemic Acute Respiratory Failure Consequent to COVID-19
Authors: Vianello Andrea , De Vita Nello , Scotti Lorenza , Guarnieri Gabriella , Confalonieri Marco , Bonato Valeria , Molena Beatrice , Maestrone Carlo , Airoldi Gianluca , Olivieri Carlo , Sainaghi Pier Paolo , Lionello Federico , Arcaro Giovanna , Della Corte Francesco , Navalesi Paolo , Vaschetto Rosanna
Published: March 2022

As the clinical outcome of octogenarian patients hospitalised for COVID-19 is very poor, here we assessed the clinical characteristics and outcomes of patients aged 80 year or older hospitalised for COVID-19 receiving non-invasive respiratory support (NIRS). A multicentre, retrospective, observational study was conducted in seven hospitals in Northern Italy. All patients aged ≥80 years with COVID-19 associated hypoxemic acute respiratory failure (hARF) undergoing NIRS between 24 February 2020, and 31 March 2021, were included. Out of 252 study participants, 156 (61.9%) and 163 (64.6%) died during hospital stay and within 90 days from hospital admission, respectively. In this case, 228 (90.5%) patients only received NIRS (NIRS group), while 24 (9.5%) were treated with invasive mechanical ventilation (IMV) after NIRS failure (NIRS+IMV group). In-hospital mortality did not significantly differ between NIRS and NIRS+IMV group (61.0% vs. 70.8%, respectively; p = 0.507), while survival probability at 90 days was significantly higher for NIRS compared to NIRS+IMV patients (0.379 vs. 0.147; p = 0.0025). The outcome of octogenarian patients with COVID-19 receiving NIRS is quite poor. Caution should be used when considering transition from NIRS to IMV after NIRS failure.
Impact on Postoperative Pain and Recovery of a Regional Analgesia Strategy Based on the Surgical Approach for Lung Resection: A Prospective Observational Study
Authors: Trouillard Marion , Dupuis William , Siaudeau Hélène , Denou Florian , Longeau Emmanuelle , Léger Maxime , Ammi Myriam , Sargentini Cyril , Lasocki Sigismond , Rineau Emmanuel
Published: March 2022

Various regional anesthesia (RA) techniques were shown to reduce pain after lung surgery, but controversies remain regarding the best technique to use to improve recovery. In this observational prospective study, the aim was to assess the efficacy of an RA strategy depending on the surgical approach. Patients who underwent lung surgery were included if an RA was planned following our unit procedure (erector spinae plane block (ESP) for video-assisted thoracic surgery (VATS) and thoracic epidural analgesia (TEA) or intrathecal analgesia (IA) for thoracotomy). Patients were compared according to the RA used. In total, 116 patients were included, 70 (60%), 32 (28%), 14 (12%) in the ESP, TEA and IA groups, respectively. Between Day 1 and Day 3, median NRS values were ≤4 at rest, and <50% patients experienced moderate-to-severe pain in each group. There were no significant differences in opioid consumption and in pain at rest or during chest physiotherapy on Days 1 and 2 between groups. However, patients who received an IA had lower NRS than other groups on Day 0 and 3 and a shorter length of hospital stay in comparison with those who received a TEA. Thus, in our institution, a strategy combining ESP for VATS and TEA, or IA for thoracotomy, allowed for effective analgesia after a lung resection. Interestingly, IA appeared to be more effective than TEA in reducing the length of hospital stay and pain on Day 0 and 3.
Experimental Investigation on Machine-Induced Damages during the Milling Test of Graphene/Carbon Incorporated Thermoset Polymer Nanocomposites
Authors: Kumar Jogendra , Abhishek Kumar , Xu Jinyang , Verma Rajesh Kumar
Published: March 2022

The fiber laminate composites are extensively used in aerospace, aircraft, automotive components due to their high stiffness, corrosion, moisture resistance, low weight, and durability features. These fiber composites are modified with nanomaterials to acquire the desired manufacturing properties. The complex structure and anisotropic features differ from metals and their alloys. Additionally, the machining principles of fiber laminates significantly differ from conventional engineering materials. The present work investigates the machining behavior and permeates the damage generated while milling of graphene-modified carbon-fiber reinforced polymer nanocomposites (G/C@FRNC). The surface damages and defects caused in the milling samples have been examined through the high-resolution spectroscopy test. The influence of machining constraints such as cutting speed (N), feed rate (F), depth of cut (D), and graphene oxide weight % (GO) has been investigated to achieve the desired milling performances viz. material removal rate (MRR), cutting force (Fc), surface roughness (Ra), and delamination factor (Fd). The outcomes indicated that the cutting parameters and graphene nanomaterial prominently affects the milling responses. The addition of graphene improves the machinability of proposed nanocomposites with lesser defects generated. However, its higher addition can lead to the phenomenon of agglomeration that can reduce the machining efficiency. The damages and delamination generated in the machined sample are low at a higher cutting speed. This work suggests a new system to control the damage and defects to enhance the laminate samples’ quality and productivity.
Scour Characteristics and Equilibrium Scour Depth Prediction around a Submarine Piggyback Pipeline
Authors: Hu Ruigeng , Wang Xiuhai , Liu Hongjun , Leng Hao , Lu Yao
Published: March 2022

Local scour around a submarine piggyback pipeline in combined waves and current is investigated experimentally. Based on the experimental results, the scour evolution and scour morphology are firstly analyzed. Then, a comparison with the equilibrium scour depth Seq between the present experimental data and predicted results is conducted. After that, the correlation between the dimensionless scour timescale T* and the maximum Shields parameter θcw is investigated, and a formula is obtained to describe the variation trend between T* and θcw for different gap ratios G/D. Furthermore, the parametric study is carried out to study the effects of Reynolds number Red and θcw on Seq, respectively. The results indicate that the Seq below the piggyback pipeline increases when the gap ratio G/D increases from 0 to 0.1, and it gradually decreases when G/D > 0.1. For a given KC, the Seq increases with the increase of the ratio of velocities Ucw. In addition, when Ucw is fixed, a higher KC results in a greater Seq. The T* is closely related to θcw and G/D. The higher Red and θcw both tend to result in the greater scour depth below a piggyback pipeline in combined waves and current.
Exoskeletons for Mobility after Spinal Cord Injury: A Personalized Embodied Approach
Authors: Forte Giuseppe , Leemhuis Erik , Favieri Francesca , Casagrande Maria , Giannini Anna Maria , De Gennaro Luigi , Pazzaglia Mariella
Published: March 2022

Endowed with inherent flexibility, wearable robotic technologies are powerful devices that are known to extend bodily functionality to assist people with spinal cord injuries (SCIs). However, rather than considering the specific psychological and other physiological needs of their users, these devices are specifically designed to compensate for motor impairment. This could partially explain why they still cannot be adopted as an everyday solution, as only a small number of patients use lower-limb exoskeletons. It remains uncertain how these devices can be appropriately embedded in mental representations of the body. From this perspective, we aimed to highlight the homeostatic role of autonomic and interoceptive signals and their possible integration in a personalized experience of exoskeleton overground walking. To ensure personalized user-centered robotic technologies, optimal robotic devices should be designed and adjusted according to the patient’s condition. We discuss how embodied approaches could emerge as a means of overcoming the hesitancy toward wearable robots.
COVID-19 and the Mortgage Market in Luxembourg
Authors: Koulischer François , Perray Pauline , Tran Thi Thu Huyen
Published: March 2022

With a ratio of household debt to gross disposable income above 150%, households in Luxembourg are among the most indebted in Europe. A high level of debt exacerbates the sensitivity of household net worth to changes in house prices, which can increase the severity of economic downturns. In this note, we evaluate the implications of the COVID-19 crisis for the mortgage market in Luxembourg using data on the labour market and government interventions, as well as surveys of consumer finances (HFCS). Our conclusions are twofold. At the aggregate level, the Luxembourg mortgage market is relatively well placed to weather the shock, because a large share of residents work in sectors that are less affected by the crisis such as the financial or government sectors. However, our analysis of micro-level survey data suggests that some segments of the population may be financially vulnerable to the COVID-19 shock.
Impact of Financial Innovation and Institutional Quality on Financial Development in Emerging Markets
Authors: Alawi Suha Mahmoud , Abbassi Wajih , Saqib Rukhma , Sharif Madeeha
Published: March 2022

Financial innovation and institutional quality play a key role in financial development. This study investigates the impact of financial innovation and institutional quality on financial development in an emerging markets setting. We used the sample of 17 emerging markets based on the availability of data from the period 1990–2020. Data were extracted from the World Development Indicator database. In this study, panel unit root, fully modified ordinary least squares and Pedroni Integration tests were applied to analyze the data. We find that financial innovation and institutional quality are significantly and positively related to financial development. Better financial innovation increases financial development, whereas low institutional quality in an emerging market can deteriorate financial development.
Can Cooperative Supports and Adoption of Improved Technologies Help Increase Agricultural Income? Evidence from a Recent Study
Authors: Khan Nawab , Ray Ram L. , Kassem Hazem S. , Ihtisham Muhammad , Siddiqui Badar Naseem , Zhang Shemei
Published: March 2022

Global climate change may result in major environmental issues that have already affected and will continue to affect agricultural sector in the future. A continuing effort to utilize and adopt new agricultural technologies is necessary to mitigate climate change and increase agricultural income. Agricultural cooperatives are gradually being used in emerging countries to encourage improved technology and reduce food insecurity and poverty. This research analyses the influence of cooperative supports (CS) and technology adoption (TA) on agricultural income in Pakistan. It applied the propensity score matching (PSM) technique to evaluate the productivity on survey data from 498 wheat growers to conduct counterfactual analysis for farmers in Pakistan. In addition, a dual selection model (DSM) was applied to resolve the bias in sample selection caused by observed and unobserved aspects of survey data. The results showed that, contrasted with non-membership and non-adopters, growers who joined CS and TA could boost agricultural income by 2.78% and 2.35%, respectively. Stimulatingly, the influence of less-revenue farmers on agricultural income was more substantial than that of high-income farmers. Agricultural income of growers who attached cooperatives and adopted improved agricultural technology enhanced by 5.45% and 4.51%, respectively. These results, among others, emphasize the optimistic role of growing CS and TA in boosting wheat farmer’s income. The findings of the study showed strong relationships among education, age, skill, training, gender with CS and TA, and agricultural income. Overall, this study can be helpful in conducting similar studies in other emerging/developing countries for wheat or any other crop growers.
Scenario Analysis of Livestock Carrying Capacity Risk in Farmland from the Perspective of Planting and Breeding Balance in Northeast China
Authors: Zhao Zhe , Deng Xiangzheng , Zhang Fan , Li Zhihui , Shi Wenjiao , Sun Zhigang , Zhang Xuezhen
Published: March 2022

In this paper, we selected the northeast region as a study area from the perspective of soil nutrient demand, calculated the livestock carrying capacity of farmland under three scenarios where nitrogen nutrient accounts for 35% (low level), 45% (medium level), and 55% (high level) of fertilization, and carried out a risk analysis. The results show that the scale of husbandry breeding is expanding and the scale of the planting industry has remained basically unchanged. Under the three scenarios, there were 23 regions where the livestock manure exceeded the maximum value that could be absorbed by farmland in 2008 and 28 regions in 2019. These regions in the potential area are mostly located in Heilongjiang province and the regions in the restricted area are mostly located in Liaoning Province. On the whole, the northeast region is generally faced with the problem of livestock overloading, and the insufficient utilization and treatment capacity of livestock manure poses a huge threat to regional ecological security. Based on this, adjusting the structure of regional planting and breeding, promoting the development of the livestock manure processing industry, enhancing the production capacity of organic fertilizer, and constructing an integrated pattern of regional planting and breeding are effective ways to realize the sustainable utilization of farmland in northeast China.
Impact of Radiation Quality on Microdosimetry and Chromosome Aberrations for High-Energy (>250 MeV/n) Ions
Authors: Poignant Floriane , Plante Ianik , Crespo Luis , Slaba Tony
Published: March 2022

Studying energy deposition by space radiation at the cellular scale provides insights on health risks to astronauts. Using the Monte Carlo track structure code RITRACKS, and the chromosome aberrations code RITCARD, we performed a modeling study of single-ion energy deposition spectra and chromosome aberrations for high-energy (>250 MeV/n) ion beams with linear energy transfer (LET) varying from 0.22 to 149.2 keV/µm. The calculations were performed using cells irradiated directly by mono-energetic ion beams, and by poly-energetic beams after particle transport in a digital mouse model, representing the radiation exposure of a cell in a tissue. To discriminate events from ion tracks directly traversing the nucleus, to events from δ-electrons emitted by distant ion tracks, we categorized ion contributions to microdosimetry or chromosome aberrations into direct and indirect contributions, respectively. The ions were either ions of the mono-energetic beam or secondary ions created in the digital mouse due to interaction of the beam with tissues. For microdosimetry, the indirect contribution is largely independent of the beam LET and minimally impacted by the beam interactions in mice. In contrast, the direct contribution is strongly dependent on the beam LET and shows increased probabilities of having low and high-energy deposition events when considering beam transport. Regarding chromosome aberrations, the indirect contribution induces a small number of simple exchanges, and a negligible number of complex exchanges. The direct contribution is responsible for most simple and complex exchanges. The complex exchanges are significantly increased for some low-LET ion beams when considering beam transport.
The Role of Lysine Methyltransferase SET7/9 in Proliferation and Cell Stress Response
Authors: Daks Alexandra , Vasileva Elena , Fedorova Olga , Shuvalov Oleg , Barlev Nickolai A.
Published: March 2022

Lysine-specific methyltransferase 7 (KMT7) SET7/9, aka Set7, Set9, or SetD7, or KMT5 was discovered 20 years ago, yet its biological role remains rather enigmatic. In this review, we analyze the particularities of SET7/9 enzymatic activity and substrate specificity with respect to its biological importance, mostly focusing on its two well-characterized biological functions: cellular proliferation and stress response.
O-((Ferrocenyl)(3-fluorophenyl)methyl)hydroxylamine
Authors: Foscolos Angeliki S. , Georgiou Maria , Papadopoulos Minas S. , Chiotellis Aristeidis
Published: March 2022

Based on the diaryl hydroxylamine scaffold, which exhibits the potential to inhibit all three enzymes of the first step of the kynurenine pathway, the main tryptophan degradation pathway in mammals, which is often activated in cancer, we report herein the synthesis of a ferrocenyl analogue as an attempt to improve the scaffold’s pan-inhibitory potency through the isosteric replacement of a phenyl group with the ferrocenyl moiety. The synthetic methodology followed gives access to O-((ferrocenyl)(aryl)methyl)hydroxylamines, a class of compounds not yet reported in the literature.
Design and Characterization of Asymmetric Cell Structure of Auxetic Material for Predictable Directional Mechanical Response
Authors: Valle Rodrigo , Pincheira Gonzalo , Tuninetti Víctor , Fernandez Eduardo , Uribe-Lam Esmeralda
Published: March 2022

A three-dimensional auxetic structure based on a known planar configuration including a design parameter producing asymmetry is proposed in this study. The auxetic cell is designed by topology analysis using classical Timoshenko beam theory in order to obtain the required orthotropic elastic properties. Samples of the structure are fabricated using the ABSplus fused filament technique and subsequently tested under quasi-static compression to statistically determine the Poisson’s ratio and Young’s modulus. The experimental results show good agreement with the topological analysis and reveal that the proposed structure can adequately provide different elastic properties in its three orthogonal directions. In addition, three point bending tests were carried out to determine the mechanical behavior of this cellular structure. The results show that this auxetic cell influences the macrostructure to exhibit different stiffness behavior in three working directions.