bluuebunny/crossref_metadata_2025 huggingface dataset:
User Association Performance Trade-Offs in Integrated RF/mmWave/THz Communications
Authors: Hassan Noha , Fernando Xavier , Woungang Isaac , Anpalagan Alagan
Published: November 2023

In combination with the expected traffic avalanche foreseen for the next decade, solutions supporting energy-efficient, scalable and flexible network operations are essential. Considering the myriad of user case requirements, THz and mmW bands will play key roles in 6G networks. While mmW is known for short-rate LOS connections, THz transmission is subjected to even severe propagation losses, resulting in very short-range connections. In this context, we evaluate a dynamic multi-band user association algorithm to optimize connectivity in coexisting RF/mmW/THz networks. The algorithm periodically calculates association scores for each user–base station pair based on real-time channel conditions across bands, accounting for factors like signal strength, link blockage risk and noise. It then reassociates users in batches to balance loads while considering user priorities and network conditions. We simulate the algorithm’s performance within a realistic propagation model, where high path loss, molecular absorption, blockage, and narrow beam widths contribute to lower coverage at higher frequencies. Results demonstrate the algorithm’s ability to efficiently utilize network resources across diverse operating environments. In addition, our results show that the choice of frequency band depends on the specific requirements of the application, the environment, and the trade-offs between coverage distance, capacity, and interference conditions.
The Effects of Fire Severity on Vegetation Structural Complexity Assessed Using SAR Data Are Modulated by Plant Community Types in Mediterranean Fire-Prone Ecosystems
Authors: Jimeno-Llorente Laura , Marcos Elena , Fernández-Guisuraga José Manuel
Published: November 2023

Vegetation structural complexity (VSC) plays an essential role in the functioning and the stability of fire-prone Mediterranean ecosystems. However, we currently lack knowledge about the effects of increasing fire severity on the VSC spatial variability, as modulated by the plant community type in complex post-fire landscapes. Accordingly, this study explored, for the first time, the effect of fire severity on the VSC of different Mediterranean plant communities one year after fire by leveraging field inventory and Sentinel-1 C-band synthetic aperture radar (SAR) data. The field-evaluated VSC retrieved in post-fire scenarios from Sentinel-1 γ0 VV and VH backscatter data featured high fit (R2 = 0.878) and low predictive error (RMSE = 0.112). Wall-to-wall VSC estimates showed that plant community types strongly modulated the VSC response to increasing fire severity, with this response strongly linked to the regenerative strategies of the dominant species in the community. Moderate and high fire severities had a strong impact, one year after fire, on the VSC of broom shrublands and Scots pine forests, dominated by facultative and obligate seeder species, respectively. In contrast, the fire-induced impacts on VSC were not significantly different between low and moderate fire-severity scenarios in communities dominated by resprouter species, i.e., heathlands and Pyrenean oak forests.
Enhancing Fire Safety: Real-Scale Experimental Analysis of External Thermal Insulation Composite System Façades’ Behavior in Fire
Authors: Bode Florin , Simion Adrian , Anghel Ion , Sandu Mihnea , Banyai Daniel
Published: November 2023

In the unfortunate event of a fire, within the context of the evolution of façade fires, with a specific focus on the utilization of polystyrene thermal insulation (external thermal insulation composite system façades—ETICS façades), this study delves into the investigation of fires ignited by containers containing plastic bottles. Through an examination of the fluctuating temperatures within the affected room and its adjacent areas, as well as an assessment of the fire’s impact on polystyrene thermal insulation, this paper underscores the significance of incorporating non-combustible barriers into the building’s thermal insulation system. The tests conducted revealed that the temperature inside the room reached a maximum of 1100 °C, subsequently decreasing to 800 °C at a height of 2.5 m and approximately 400 °C at a height of 5 m. For this research, two 1100-L containers of household waste were employed, each weighing 45.5 kg and possessing a gross calorific value of 46.97 MJ/kg, with 10.7 kg of PET bottles inside, characterized by a higher calorific value of 23.90 MJ/kg as the source of the fire. Heat release rate highest values were obtained between 11 and 17 min, with a maximum value of 4919 kW. Thus, even in the absence of specific legislation, this study emphasizes the imperative need to establish safety distances for the storage of household waste away from the building’s façade to mitigate the risk of fire propagation, particularly in relation to materials such as polystyrene thermal insulation. Furthermore, in certain situations, extensive fire experiments on a grand scale, like the one undertaken in this research, hold a crucial position in confirming numerical findings for global researchers. This process assures the reliability and real-world usefulness of fire safety studies through the experimental outcomes presented in this investigation.
Exogenous β-Propeller Phytase and Prebiotic Mannan Oligosaccharide (MOS) Supplementation of Formulated Diets Applied to Juvenile Nile Tilapia, Oreochromis niloticus: Impact on Growth Performance and Nutrient Digestibility
Authors: Al-Ghamdi Othman Ahmed , Saïdi Saber Abdelkader , Farhat-Khemakhem Ameny , Ghorbel Sofiane , Chouayekh Hichem , Cerruti Pierfrancesco , Azaza Mohamed Salah
Published: November 2023

The growth of the aquafeed sector is highly dependent upon the availability of fish feed with a balanced nutritional composition. The use of prebiotics and probiotics can be an effective solution to increase the bioavailability of feed components. In this study, we evaluated the effect of dietary supplementation with β-propeller phytase (0, 600, 1200, 1800 and 2400 U/kg) from Bacillus and mannan oligosaccharide (MOS) (0, 2, 4 and 8 g/kg) on growth performance and nutrient digestibility of Nile tilapia over 45 days. The findings showed that adding phytase significantly (p < 0.05) increases the growth performance and nutrient digestibility; the 1200 and 1800 U/kg PHY levels showed the maximum weight gain (WG), specific growth rate (SGR) and protein efficiency ratio (PER) and best feed conversion ratio (FCR). Furthermore, phytase increases carcass mineral composition (phosphate and calcium). At the end of the experiment, there were no significant differences among all feeding groups in survival rates (above 90%). Regarding MOS inclusion, insignificant differences were seen in WG, SGR and SR. However, significant effects of MOS were observed on FCR, feed intake (FI) and PER when supplemented at 4 and 8 g/kg of feed. Taken together, our results suggest that supplementation of Nile tilapia feed with adequate amounts of β-propeller phytase from Bacillus and MOS increases growth performance and nutrient digestibility.
Insects as Valuable Sources of Protein and Peptides: Production, Functional Properties, and Challenges
Authors: Hasnan Fatin Fayuni Binti , Feng Yiming , Sun Taozhu , Parraga Katheryn , Schwarz Michael , Zarei Mohammad
Published: November 2023

As the global population approaches 10 billion by 2050, the critical need to ensure food security becomes increasingly pronounced. In response to the urgent problems posed by global population growth, our study adds to the growing body of knowledge in the field of alternative proteins, entomophagy, insect-based bioactive proteolysates, and peptides. It also provides novel insights with essential outcomes for guaranteeing a safe and sustainable food supply in the face of rising global population demands. These results offer insightful information to researchers and policymakers tackling the intricate relationship between population expansion and food supplies. Unfortunately, conventional agricultural practices are proving insufficient in meeting these demands. Pursuing alternative proteins and eco-friendly food production methods has gained urgency, embracing plant-based proteins, cultivated meat, fermentation, and precision agriculture. In this context, insect farming emerges as a promising strategy to upcycle agri-food waste into nutritious protein and fat, meeting diverse nutritional needs sustainably. A thorough analysis was conducted to evaluate the viability of insect farming, investigate insect nutrition, and review the techniques and functional properties of protein isolation. A review of peptide generation from insects was conducted, covering issues related to hydrolysate production, protein extraction, and peptide identification. The study addresses the nutritional value and global entomophagy habits to elucidate the potential of insects as sources of peptides and protein. This inquiry covers protein and hydrolysate production, highlighting techniques and bioactive peptides. Functional properties of insect proteins’ solubility, emulsification, foaming, gelation, water-holding, and oil absorption are investigated. Furthermore, sensory aspects of insect-fortified foods as well as challenges, including Halal and Kosher considerations, are explored across applications. Our review underscores insects’ promise as sustainable protein and peptide contributors, offering recommendations for further research to unlock their full potential.
Agri-Food Value Chain Traceability Using Blockchain Technology: Portuguese Hams’ Production Scenario
Authors: Arvana Miguel , Rocha Andre Dionisio , Barata Jose
Published: November 2023

The globalization of food markets has led companies to buy products not only locally, but also from other corners of the world. This has introduced complexity into supply chains, as products have to move longer distances and pass through more companies before reaching the end consumer. The meat industry has been no different. Events such as animal disease outbreaks have diminished consumer confidence in the industry and the supply chain. Coupled with this, consumers started demanding “more transparent” meat products. This has led companies to think about new traceability systems, which continue to enforce food safety and health rules, but at the same time enhance and make transparent to the consumer the origin and constitution of their products. This article proposes a traceability system in the agri-food (meat industry) with a multi-chain architecture, among them, blockchain. The use of blockchain in the traceability system helped to mitigate the omission of relevant data for the traceability process, allowing us to guarantee the immutability, reliability, and transparency of the data along the value chain. At the same time, the system was able to reduce the time of the traceability process by giving the user the possibility to access the traced information via a unique product identifier.
Natural Food Resource Valorization by Microwave Technology: Purslane Stabilization by Dielectric Heating
Authors: Apicella Marco , Amato Giuseppe , de Bartolomeis Pietro , Barba Anna Angela , De Feo Vincenzo
Published: November 2023

The application of microwave-assisted drying is a promising technique due to the features of process sustainability that are usable for responsible productions. It is largely applied for the stabilization of food products, especially in the agro-food sector. In this study, the weed Portulaca oleracea L. (purslane), with its richness in antioxidant components in addition to its recognized pharmacological properties, has been considered due to its potential to be a natural, well-accepted future food. Attention was focused on the role of the heat and mass transfer rates involved in the drying processes on the nutritional profile of the dried products. For this purpose, different drying protocols (convective, microwave irradiation, microwave-vacuum irradiation) were applied to different parts of purslane herb (apical, twigs, entire structures) and chemical characterizations were performed by a GC/MS analysis of the extracts of the dried products. The results show that microwave treatments can assure a better preservation of fatty acids such as SFAs, MUFAs, and PUFAs (which constitute over 90% of the total components in the apical part, 65% in twigs, and 85% in microwave-vacuum-dried entire purslane samples) and phytosterols (their highest preservation was found in microwave-dried twigs) compared with convective treatments. The chemical composition variability as well as treatment times depend on the drying rates (in microwave treatments, the times are on a minute scale and the rates are up to three orders of magnitude greater than convective ones), which in turn depend on the heating transport phenomena. This variability can lead towards products that are diversified by properties that transform a weed into a valorized food source.
Morin Treatment Delays the Ripening and Senescence of Postharvest Mango Fruits
Authors: Guo Lihong , Liang Kaiqi , Huang Xiaochun , Mai Weiqian , Duan Xuewu , Wu Fuwang
Published: November 2023

A 0.005% and 0.01% morin treatment was applied to treat mango fruits stored under ambient conditions (25 ± 1 °C) with 85–90% relative humidity, and the effects on quality indexes, enzyme activity related to antioxidation and cell wall degradation, and gene expressions involved in ripening and senescence were explored. The results indicate that a 0.01% morin application effectively delayed fruit softening and yellowing and sustained the nutritional quality. After 12 days of storage, the contents of soluble sugar and carotenoid in the treatment groups were 68.54 mg/g and 11.20 mg/100 g, respectively, lower than those in control, while the vitamin C content in the treatment groups was 0.58 mg/g, higher than that in control. Moreover, a morin application successively enhanced the activity of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), but reduced the activity of polygalacturonase (PG) and pectin lyase (PL). Finally, real-time PCR and correlation analysis suggested that morin downregulated the ethylene biosynthesis (ACS and, ACO) and signal transduction (ETR1, ERS1, EIN2, and ERF1) genes, which is positively associated with softening enzymes (LOX, EXP, βGal, and EG), carotenoid synthesis enzymes (PSY and, LCYB), sucrose phosphate synthase (SPS), and uncoupling protein (UCP) gene expressions. Therefore, a 0.01% morin treatment might efficiently retard mango fruit ripening and senescence to sustain external and nutritional quality through ethylene-related pathways, which indicates its preservation application.
Highly Sensitive Immunochromatographic Detection of Porcine Myoglobin as Biomarker for Meat Authentication Using Prussian Blue Nanozyme
Authors: Hendrickson Olga D. , Zvereva Elena A. , Dzantiev Boris B. , Zherdev Anatoly V.
Published: November 2023

This study was aimed at the sensitive immunodetection of porcine myoglobin (MG) as a species-specific biomarker in meat products. The enhanced lateral flow immunoassay (LFIA) was created in the sandwich format using monoclonal antibodies (Mab) with specificity to porcine MG and labeled by Prussian blue nanoparticles (PBNPs) as peroxidase-mimicking nanozymes. Signal amplification was provided by the colored product of oxidation catalyzed by the PBNPs. Several Mab–PBNP conjugates with different antibody loads were synthesized; the one that provided the best analytical characteristics of the LFIA was selected. Advanced optimization of the test system was carried out. As a result, the visual limit of detection (LOD) of MG was 1.5 ng/mL. Involvement of the catalytic nanozyme properties allowed the LOD to be decreased by ~9 times in comparison to the LFIA based on gold nanomarkers, and by ~27 times compared to the LFIA based on PBNP coloration. The assay time was 30 min, including catalytic enhancement. A simple technique of meat sample pre-treatment aimed at effective MG extraction and matrix disposal was proposed. The specificity of the LFIA towards the pork meat was demonstrated. The applicability of the created test system was shown by testing extracts obtained from finished meat products.
Qualitative Aspects of a Fractional-Order Integro-Differential Equation with a Quadratic Functional Integro-Differential Constraint
Authors: El-Sayed Ahmed M. A. , Alhamali Antisar A. A. , Hamdallah Eman M. A. , Ebead Hanaa R.
Published: November 2023

This manuscript investigates a constrained problem of an arbitrary (fractional) order quadratic functional integro-differential equation with a quadratic functional integro-differential constraint. We demonstrate that there is at least one solution x∈C[0,T] to the problem. Moreover, we outline the necessary demands for the solution’s uniqueness. In addition, the continuous dependence of the solution and the Hyers–Ulam stability of the problem are analyzed. In order to illustrate our results, we provide some particular cases and instances.
Exceptionally Fast Temperature-Responsive, Mechanically Strong and Extensible Monolithic Non-Porous Hydrogels: Poly(N-isopropylacrylamide) Intercalated with Hydroxypropyl Methylcellulose
Authors: Strachota Beata , Strachota Adam , Vratović Leana , Pavlova Ewa , Šlouf Miroslav , Kamel Samir , Cimrová Věra
Published: November 2023

Exceptionally fast temperature-responsive, mechanically strong, tough and extensible monolithic non-porous hydrogels were synthesized. They are based on divinyl-crosslinked poly(N-isopropyl-acrylamide) (PNIPAm) intercalated by hydroxypropyl methylcellulose (HPMC). HPMC was largely extracted after polymerization, thus yielding a ‘template-modified’ PNIPAm network intercalated with a modest residue of HPMC. High contents of divinyl crosslinker and of HPMC caused a varying degree of micro-phase-separation in some products, but without detriment to mechanical or tensile properties. After extraction of non-fixed HPMC, the micro-phase-separated products combine superior mechanical properties with ultra-fast T-response (in 30 s). Their PNIPAm network was highly regular and extensible (intercalation effect), toughened by hydrogen bonds to HPMC, and interpenetrated by a network of nano-channels (left behind by extracted HPMC), which ensured the water transport rates needed for ultra-fast deswelling. Moreover, the T-response rate could be widely tuned by the degree of heterogeneity during synthesis. The fastest-responsive among our hydrogels could be of practical interest as soft actuators with very good mechanical properties (soft robotics), while the slower ones offer applications in drug delivery systems (as tested on the example of Theophylline), or in related biomedical engineering applications.
The Systematics, Reproductive Biology, Biochemistry, and Breeding of Sea Buckthorn—A Review
Authors: Nybom Hilde , Ruan Chengjiang , Rumpunen Kimmo
Published: November 2023

Both the fruit flesh and seeds of sea buckthorn have multiple uses for medicinal and culinary purposes, including the valuable market for supplementary health foods. Bioactive compounds, such as essential amino acids, vitamins B, C, and E, carotenoids, polyphenols, ursolic acid, unsaturated fatty acids, and other active substances, are now being analyzed in detail for their medicinal properties. Domestication with commercial orchards and processing plants is undertaken in many countries, but there is a large need for improved plant material with high yield, tolerance to environmental stress, diseases, and pests, suitability for efficient harvesting methods, and high contents of compounds that have medicinal and/or culinary values. Applied breeding is based mainly on directed crosses between different subspecies of Hippophae rhamnoides. DNA markers have been applied to analyses of systematics and population genetics as well as for the discrimination of cultivars, but very few DNA markers have as yet been developed for use in selection and breeding. Several key genes in important metabolic pathways have, however, been identified, and four genomes have recently been sequenced.
Unraveling the Genetic Basis of Feed Efficiency in Cattle through Integrated DNA Methylation and CattleGTEx Analysis
Authors: Hu Zhenbin , Boschiero Clarissa , Li Cong-Jun , Connor Erin E. , Baldwin Ransom L. , Liu George E.
Published: November 2023

Feed costs can amount to 75 percent of the total overhead cost of raising cows for milk production. Meanwhile, the livestock industry is considered a significant contributor to global climate change due to the production of greenhouse gas emissions, such as methane. Indeed, the genetic basis of feed efficiency (FE) is of great interest to the animal research community. Here, we explore the epigenetic basis of FE to provide base knowledge for the development of genomic tools to improve FE in cattle. The methylation level of 37,554 CpG sites was quantified using a mammalian methylation array (HorvathMammalMethylChip40) for 48 Holstein cows with extreme residual feed intake (RFI). We identified 421 CpG sites related to 287 genes that were associated with RFI, several of which were previously associated with feeding or digestion issues. Activator of transcription and developmental regulation (AUTS2) is associated with digestive disorders in humans, while glycerol-3-phosphate dehydrogenase 2 (GPD2) encodes a protein on the inner mitochondrial membrane, which can regulate glucose utilization and fatty acid and triglyceride synthesis. The extensive expression and co-expression of these genes across diverse tissues indicate the complex regulation of FE in cattle. Our study provides insight into the epigenetic basis of RFI and gene targets to improve FE in dairy cattle.
Verification of C-Band Geophysical Model Function for Wind Speed Retrieval in the Open Ocean and Inland Water Conditions
Authors: Sergeev Daniil , Ermakova Olga , Rusakov Nikita , Poplavsky Evgeny , Gladskikh Daria
Published: November 2023

Verification of the C-band geophysical model functions (GMF) for the open ocean and the Gorky reservoir was carried out using Sentinel-1 IW-mode Synthetic aperture radar (SAR) images. CMOD5.N, CMOD7, GMF for the Caspian Sea, and CSARMOD2 were considered. The motivation for this study is concerned with the clarification of applying C-band GMFs for SAR images including for the conditions of inland water bodies, as well as with the study of the influence of various wind speed direction sources on the results of wind speed magnitude retrieval for ocean conditions. Comparison of wind speed from the CMOD5.N algorithm using wind direction data from NOAA NDBC oceanographic buoys together with the data provided by NCEP reanalysis data showed that regardless of the geographic location, the result does not depend significantly on the choice of the wind direction source. Novel results of CMOD5.N, CMOD7, GMF for the Caspian Sea, and CSARMOD2 applications to the conditions of the Gorky reservoir are presented. The comparison of these results with the meteorological station measurements showed the best agreement for CMOD5.N. The preliminary results on the construction of new C-band GMF adjusted to the Gorky Reservoir have shown statistical parameters better than for Caspian Sea GMF and CSARMOD2.
Gut–Brain Axis, Microbiota and Probiotics—Current Knowledge on Their Role in Irritable Bowel Syndrome: A Review
Authors: Marginean Cristina Maria , Popescu Mihaela , Drocas Andrei Ioan , Cazacu Sergiu Marian , Mitrut Radu , Marginean Iulia Cristina , Iacob George Alexandru , Popescu Marian Sorin , Docea Anca Oana , Mitrut Paul
Published: November 2023

Irritable bowel syndrome (IBS) is a common digestive disorder with a significant impact on both individuals and society in terms of quality of life and healthcare costs. A growing body of research has identified various communication pathways between the microbiota and the brain in relation to motility disorders, with the gut–brain axis being key to the pathogenesis of IBS. Multiple factors contribute to the pathogenetic pathways in IBS, including immune mechanisms, psychosocial factors, increased oxidative stress and pro-inflammatory cytokine release, as well as genetic and hormonal factors. Increased permeability of the normal intestinal barrier allows bacterial products to access the lamina propria, providing a mechanism for perpetuating chronic inflammation and characteristic symptoms. The microbiota influences inflammatory processes in IBS by altering the balance between pro-inflammatory factors and host defence. Probiotics modulate the pathophysiological mechanisms involved in IBS by influencing the composition of the microbiota and improving intestinal motility disorders, visceral hypersensitivity, immune function of the intestinal epithelium, metabolic processes in the intestinal lumen, dysfunction of the microbiota-GBA, and are recognised as effective and safe in IBS therapy. Our study aimed to provide a comprehensive overview of the relationship between the gut–brain axis, microbiota, and IBS, based on current information.
A Life Cycle Cost Analysis—Relevant Method Supporting the Decision to Establish an Apple Orchard in an Organic System
Authors: Vlad Ionela Mițuko , Butcaru Ana Cornelia , Fîntîneru Gina , Bădulescu Liliana Aurelia , Stănică Florin , Mihai Cosmin Alexandru
Published: November 2023

The life-cycle cost analysis is a method used to assess long-term economic efficiency among equivalent competing processes or products. The purpose of this paper is to investigate the nature and level of costs for an organic orchard located in Southern Romania, using a complex approach covering the entire chain of production, through its life span. The research results, based on a dynamic analysis and an integrated evaluation of the orchard’s performance, were ranked on investment and operational costs and broken down into three categories (establishment, production, post-harvest, transport costs). The highest costs, representing 151,726 EUR/ha/20 years, about 52.72% of the total operational costs and 50.4% of the total farm costs/ha/20 years, were recorded in the exploitation stage. The scenarios for the sensitivity analysis considered different levels of average yields (40 and 60 tons/ha, respectively) with different rates of sold productions (85%, optimistic scenario; 70%, pessimistic scenario). The hot points identified at the production stage were the use of agricultural machinery, several pesticides, the costs of seedlings, anti-hail nets, plastic boxes, and labor costs, while at the post-harvest stage, there were those related to labor and energy consumption. The transport stage had important costs with respect to tractor operations and the track.
Phloem-Expressed CLAVATA3/ESR-like Genes in Potato
Authors: Gancheva Maria S. , Losev Maxim R. , Dodueva Irina E. , Lutova Lyudmila A.
Published: November 2023

In potato, phloem tissues transport sugars and signal molecules to the tuber for growth and storage. The CLAVATA3/ESR-like (CLE) family of plant peptides plays an important role in regulating plant development. In this study, we identified a set of phloem-expressed CLE genes in Solanum tuberosum L. (StCLEs). We analyzed the phloem transcriptome of potato and found that 10 out of 41 StCLE genes were expressed in phloem cells, with StCLE12 and StCLE19 showing the highest expression levels. StCLE12 has an identical CLE domain to the Arabidopsis TDIF peptides, which are known to play a crucial role in maintaining the vascular meristem. StCLE19 has the highest sequence similarity to the Arabidopsis CLE25 peptide, which is involved in the formation of the phloem element and signaling in response to dehydration stress. The overexpression of StCLE12 and another potato TDIF-like gene, StCLE8, promoted vascular cell proliferation and delayed leaf senescence. On the other hand, plants with overexpression of StCLE19 were unable to form adventitious roots and demonstrated the absence of ordered cambium cell layers in the vascular bundles.
Evaluation of Baseflow Modeling with BlueM.Sim for Long-Term Hydrological Studies in the German Low Mountain Range of Hesse, Germany
Authors: Kissel Michael , Bach Michael , Schmalz Britta
Published: November 2023

So far, research with the hydrological model BlueM.Sim has been focused on reservoir management and integrated river basin modeling. BlueM.Sim is part of the official toolset for estimating immissions into rivers in Hesse (Germany) via long-term continuous modeling. Dynamic runoff modeling from rural catchments is permitted within the Hessian guidelines, but in practice, a constant flow or low flow is used. However, due to increasing water stress in the region caused by climate change, the dynamic modeling of runoff from rural catchments will become necessary. Therefore, dynamic baseflow modeling with BlueM.Sim is of the greatest importance. This study evaluated baseflow modeling with BlueM.Sim in a representative hard-rock aquifer in the German Low Mountain range. Two model setups (Factor Approach (FA): CN method + monthly baseflow; Soil Moisture Approach (SMA): physical soil moisture simulation) were calibrated (validated) for a 9-year (5-year) period. The FA achieved an NSE of 0.62 (0.44) and an LnNSE of 0.64 (0.60) for the calibration and validation periods. The selection of a solution for the successful validation of the FA was challenging and required a selection that overestimated baseflow in the calibration period. This is due to the major disadvantage of the FA, namely, that baseflow can only vary according to an estimated yearly pattern of monthly baseflow factors. However, the data requirements are low, and the estimation of monthly baseflow factors is simple and could potentially be regionalized for Hesse, leading to a better representation of baseflow than in current practice. The SMA achieved better results with an NSE of 0.78 (0.75) and an LnNSE of 0.72 (0.78). The data requirements and model setup are extensive and require the estimation of many parameters, which are limitations to its application in practice. Furthermore, a literature review has shown that a single linear reservoir, as in BlueM.Sim, is not optimal for modeling baseflow in hard-rock aquifers. However, for detailed climate change impact studies in the region with BlueM.Sim, the SMA should be preferred over the FA. It is expected that BlueM.Sim would benefit from implementing a more suitable model structure for baseflow in hard-rock aquifers, resulting in improved water balance and water quality outcomes.
Multi-Level Perceptions on Higher Education Development for Sanitation and Hygiene Management in Nigeria
Authors: Cookey Peter Emmanuel , Peter-Cookey Mayowa Abiodun , Richard Iloma Unwobuesor , Brdanovic Damir
Published: November 2023

Providing safely managed sanitation/hygiene requires key competencies for education, training, service delivery, enterprise development and management, product and infrastructure design and development, construction and installation, governance, financing, research, etc. These strategic sanitation capacities will have to be built from higher education’s academic and professional programmes structured and designed to produce skilled and knowledgeable professionals and practitioners. This study aims to investigate the quality of the sanitation/hygiene management content of Environmental Health programmes; the adequacy of the existing central curricula; and the perception of environmental health sanitation/hygiene-trained professionals to determine the next phase for building knowledge and capacity of sanitation professionals in Nigeria through higher education institutions. A multi-level mixed method concurrent study was used for sampling and data collection with a multi-level perception analysis to examine the perceptions of students, lecturers and graduate alumni, as well as employers/supervisors and clients/service users of sanitation/hygiene-related graduates. The main findings of this paper show a limited understanding of the concepts of contemporary issues of sanitation/hygiene management like sustainable sanitation, citywide inclusive sanitation, regenerative sanitation, circular bioeconomy, etc.; the central/national teaching and curricula were found to be seriously out of date; and clients/service users were dissatisfied with the skills and knowledge levels of the graduates. The study concludes by recommending a national sanitation management higher education pathway to guide the provision of integrated sanitation/hygiene management education at higher education institutions (HEIs), to build an effective, efficient, competent and sufficient workforce for the country.
Analysis of the Functioning of Families of Children with Autism Spectrum Disorder: A Psychometric Study of the Family APGAR Scale
Authors: Serrano Laura , Vela Esther , Martín Laura
Published: November 2023

Normalised family functioning is a predictor of individual well-being. The diagnosis of a family member with autism spectrum disorder (ASD) can alter the ordinary dynamics of family systems, having a variable impact on family functioning. This research employed a non-probability convenience sampling method to gather a sample of 327 families with and without children diagnosed with ASD. This study has dual objectives: to analyse the psychometric properties of the Spanish version of the Family APGAR Scale and to compare family functioning in families with and without a child with ASD. The results reveal several favourable psychometric properties in the application of the APGAR scale within families of children with ASD. The use of the Family APGAR Scale in the selected sample confirms that the functioning of families with children with ASD can be categorized as mildly dysfunctional, attributed to the inherent challenges in caring for and raising a child with ASD. The presence of ASD within family systems presents a challenge to typical family functioning, with significant differences observed between families with and without children with ASD. This underscores the necessity of implementing effective intervention programs based on empirical evidence to improve the quality of life for individuals with ASD and their families.
Being Female and in a Romantic Relationship Enhances the Association between Satisfaction with Love Life and Capacity to Love
Authors: Leite Ângela , Costa Ana , Ribeiro Beatriz , Fonseca Carolina , Ribeiro Inês , Mesquita Joana , Ribeiro Sara
Published: November 2023

This study aims to evaluate if the relation between love life satisfaction, assessed by the satisfaction with love life scale (SWLLS), and capacity to love, assessed by the capacity to love inventory (CTL-I), is moderated by gender and by being or not in a romantic relationship, in a Portuguese sample. To this end, the adaptation and validation of CTL-I for this population were carried out through an exploratory factorial analysis (EFA) followed by a Robust Maximum Likelihood (MLR) confirmatory factorial analysis (CFA). A multi-group analysis for measurement invariance depending on being in a romantic relationship or not was assessed. The model’s reliability was also evaluated. The associations between SWLLS and CTL-I were tested by correlations, regressions and moderations. At last, differences between means and distributions concerning sociodemographic variables were determined. The results showed that a good model fit for the Portuguese version of the CTL-I was found, as well as good psychometric properties. Results also showed that satisfaction with love life contributes to explaining the capacity to love and all its dimensions, and that gender and being in a romantic relationship moderate the association between love life satisfaction and the capacity to love. Being female and being in a romantic relationship make the relationship between love life satisfaction and the capacity to love stronger and more meaningful.
Ouabain’s Influence on TRPV4 Channels of Epithelial Cells: An Exploration of TRPV4 Activity, Expression, and Signaling Pathways
Authors: Ponce Arturo , Larre Isabel , Jimenez Lidia , Roldán Maria Luisa , Shoshani Liora , Cereijido Marcelino
Published: November 2023

Ouabain, a substance originally obtained from plants, is now classified as a hormone because it is produced endogenously in certain animals, including humans. However, its precise effects on the body remain largely unknown. Previous studies have shown that ouabain can influence the phenotype of epithelial cells by affecting the expression of cell–cell molecular components and voltage-gated potassium channels. In this study, we conducted whole-cell clamp assays to determine whether ouabain affects the activity and/or expression of TRPV4 channels. Our findings indicate that ouabain has a statistically significant effect on the density of TRPV4 currents (dITRPV4), with an EC50 of 1.89 nM. Regarding treatment duration, dITRPV4 reaches its peak at around 1 h, followed by a subsequent decline and then a resurgence after 6 h, suggesting a short-term modulatory effect related to on TRPV4 channel activity and a long-term effect related to the promotion of synthesis of new TRPV4 channel units. The enhancement of dITRPV4 induced by ouabain was significantly lower in cells seeded at low density than in cells in a confluent monolayer, indicating that the action of ouabain depends on intercellular contacts. Furthermore, the fact that U73122 and neomycin suppress the effect caused by ouabain in the short term suggests that the short-term induced enhancement of dITRPV4 is due to the depletion of PIP2 stores. In contrast, the fact that the long-term effect is inhibited by PP2, wortmannin, PD, FR18, and IKK16 suggests that cSrc, PI3K, Erk1/2, and NF-kB are among the components included in the signaling pathways.
Modulation of CD8+ T Cell Responses by Radiotherapy—Current Evidence and Rationale for Combination with Immune Checkpoint Inhibitors
Authors: Jeon Seung Hyuck , Song Changhoon , Eom Keun-Yong , Kim In Ah , Kim Jae-Sung
Published: November 2023

Radiotherapy for cancer has been known to affect the responses of immune cells, especially those of CD8+ T cells that play a pivotal role in anti-tumor immunity. Clinical success of immune checkpoint inhibitors led to an increasing interest in the ability of radiation to modulate CD8+ T cell responses. Recent studies that carefully analyzed CD8+ T cell responses following radiotherapy suggest the beneficial roles of radiotherapy on anti-tumor immunity. In addition, numerous clinical trials to evaluate the efficacy of combining radiotherapy with immune checkpoint inhibitors are currently undergoing. In this review, we summarize the current status of knowledge regarding the changes in CD8+ T cells following radiotherapy from various preclinical and clinical studies. Furthermore, key biological mechanisms that underlie such modulation, including both direct and indirect effects, are described. Lastly, we discuss the current evidence and essential considerations for harnessing radiotherapy as a combination partner for immune checkpoint inhibitors.
Regulatory Effects of the Kiss1 Gene in the Testis on Puberty and Reproduction in Hezuo and Landrance Boars
Authors: Shi Haixia , Yan Zunqiang , Du Hong , Tang Yuran , Song Kelin , Yang Qiaoli , Huang Xiaoyu , Wang Pengfei , Gao Xiaoli , Yang Jiaojiao , Gun Shuangbao
Published: November 2023

Kisspeptin, a neuropeptide encoded by the Kiss1 gene, combines with its receptor Kiss1R to regulate the onset of puberty and male fertility by the hypothalamic–pituitary–gonadal axis. However, little is known regarding the expression signatures and molecular functions of Kiss1 in the testis. H&E staining revealed that well-arranged spermatogonia, spermatocytes, round and elongated spermatids, and spermatozoa, were observed in 4-, 6-, and 8-month-old testes compared to 1- and 3-month-old testes of Hezuo pigs; however, these were not observed in Landrance until 6 months. The diameter, perimeter, and cross-sectional area of seminiferous tubules and the perimeter and area of the tubular lumen increased gradually with age in both pigs. Still, Hezuo pigs grew faster than Landrance. The cloning results suggested that the Hezuo pigs’ Kiss1 CDS region is 417 bp in length, encodes 138 amino acids, and is highly conserved in the kisspeptin-10 region. qRT-PCR and Western blot indicated that the expression trends of Kiss1 mRNA and protein were essentially identical, with higher expression levels at post-pubertal stages. Immunohistochemistry demonstrated that the Kiss1 protein was mainly located in Leydig cells and post-pubertal spermatogenic cells, ranging from round spermatids to spermatozoa. These studies suggest that Kiss1 is an essential regulator in the onset of puberty and spermatogenesis of boars.
Enhancing the Antimicrobial Properties of Peptides through Cell-Penetrating Peptide Conjugation: A Comprehensive Assessment
Authors: Kravchenko Sergey V. , Domnin Pavel A. , Grishin Sergei Y. , Vershinin Nikita A. , Gurina Elena V. , Zakharova Anastasiia A. , Azev Viacheslav N. , Mustaeva Leila G. , Gorbunova Elena Y. , Kobyakova Margarita I. , Surin Alexey K. , Fadeev Roman S. , Ostroumova Olga S. , Ermolaeva Svetlana A. , Galzitskaya Oxana V.
Published: November 2023

Combining antimicrobial peptides (AMPs) with cell-penetrating peptides (CPPs) has shown promise in boosting antimicrobial potency, especially against Gram-negative bacteria. We examined the CPP-AMP interaction with distinct bacterial types based on cell wall differences. Our investigation focused on AMPs incorporating penetratin CPP and dihybrid peptides containing both cell-penetrating TAT protein fragments from the human immunodeficiency virus and Antennapedia peptide (Antp). Assessment of the peptides TAT-AMP, AMP-Antp, and TAT-AMP-Antp revealed their potential against Gram-positive strains (Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), and Bacillus cereus). Peptides TAT-AMP and AMP-Antp using an amyloidogenic AMP from S1 ribosomal protein Thermus thermophilus, at concentrations ranging from 3 to 12 μM, exhibited enhanced antimicrobial activity against B. cereus. TAT-AMP and TAT-AMP-Antp, using an amyloidogenic AMP from the S1 ribosomal protein Pseudomonas aeruginosa, at a concentration of 12 µM, demonstrated potent antimicrobial activity against S. aureus and MRSA. Notably, the TAT-AMP, at a concentration of 12 µM, effectively inhibited Escherichia coli (E. coli) growth and displayed antimicrobial effects similar to gentamicin after 15 h of incubation. Peptide characteristics determined antimicrobial activity against diverse strains. The study highlights the intricate relationship between peptide properties and antimicrobial potential. Mechanisms of AMP action are closely tied to bacterial cell wall attributes. Peptides with the TAT fragment exhibited enhanced antimicrobial activity against S. aureus, MRSA, and P. aeruginosa. Peptides containing only the Antp fragment displayed lower activity. None of the investigated peptides demonstrated cytotoxic or cytostatic effects on either BT-474 cells or human skin fibroblasts. In conclusion, CPP-AMPs offer promise against various bacterial strains, offering insights for targeted antimicrobial development.
A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver Disease
Authors: Zhu Xiaoyang , Cai Jiajia , Wang Yifu , Liu Xinyu , Chen Xiaolei , Wang Haifei , Wu Zhengchang , Bao Wenbin , Fan Hairui , Wu Shenglong
Published: November 2023

The prevalence of non-alcoholic fatty liver disease (NAFLD) is increasing annually, and emerging evidence suggests that the gut microbiota plays a causative role in the development of NAFLD. However, the role of gut microbiota in the development of NAFLD remains unclear and warrants further investigation. Thus, C57BL/6J mice were fed a high-fat diet (HFD), and we found that the HFD significantly induced obesity and increased the accumulation of intrahepatic lipids, along with alterations in serum biochemical parameters. Moreover, it was observed that the HFD also impaired gut barrier integrity. It was revealed via 16S rRNA gene sequencing that the HFD increased gut microbial diversity, which enriched Colidextribacter, Lachnospiraceae-NK4A136-group, Acetatifactor, and Erysipelatoclostridium. Meanwhile, it reduced the abundance of Faecalibaculum, Muribaculaceae, and Coriobacteriaceae-UCG-002. The predicted metabolic pathways suggest that HFD enhances the chemotaxis and functional activity of gut microbiota pathways associated with flagellar assembly, while also increasing the risk of intestinal pathogen colonization and inflammation. And the phosphotransferase system, streptomycin biosynthesis, and starch/sucrose metabolism exhibited decreases. These findings reveal the composition and predictive functions of the intestinal microbiome in NAFLD, further corroborating the association between gut microbiota and NAFLD while providing novel insights into its potential application in gut microbiome research for NAFLD patients.
Dual-Pyramid Wide Residual Network for Semantic Segmentation on Cross-Style Datasets
Authors: Shen Guan-Ting , Huang Yin-Fu
Published: November 2023

Image segmentation is the process of partitioning an image into multiple segments where the goal is to simplify the representation of the image and make the image more meaningful and easier to analyze. In particular, semantic segmentation is an approach of detecting the classes of objects, based on each pixel. In the past, most semantic segmentation models were for only one single style, such as urban street views, medical images, or even manga. In this paper, we propose a semantic segmentation model called the Dual-Pyramid Wide Residual Network (DPWRN) to solve the segmentation on cross-style datasets, which is suitable for diverse segmentation applications. The DPWRN integrated the Pyramid of Kernel paralleled with Dilation (PKD) and Multi-Feature Fusion (MFF) to improve the accuracy of segmentation. To evaluate the generalization of the DPWRN and its superiority over most state-of-the-art models, three datasets with completely different styles are tested in the experiments. As a result, our model achieves 75.95% of mIoU on CamVid, 83.60% of F1-score on DRIVE, and 86.87% of F1-score on eBDtheque. This verifies that the DPWRN can be generalized and shows its superiority in semantic segmentation on cross-style datasets.
Is Automated Consent in Solid GDPR-Compliant? An Approach for Obtaining Valid Consent with the Solid Protocol
Authors: Florea Marcu , Esteves Beatriz
Published: November 2023

Personal Information Management Systems (PIMS) are acquiring a prominent role in the data economy by promoting services that help individuals to have more control over the processing of their personal data, in line with the European data protection laws. One of the highlighted solutions in this area is Solid, a new protocol that is decentralizing the storage of data, through the usage of interoperable web standards and semantic vocabularies, to empower its users to have more control over the processing of data by agents and applications. However, to fulfill this vision and gather widespread adoption, Solid needs to be aligned with the law governing the processing of personal data in Europe, the main piece of legislation being the General Data Protection Regulation (GDPR). To assist with this process, we analyze the current efforts to introduce a policy layer in the Solid ecosystem, in particular, related to the challenge of obtaining consent for processing personal data, focusing on the GDPR. Furthermore, we investigate if, in the context of using personal data for biomedical research, consent can be expressed in advance, and discuss the conditions for valid consent and how it can be obtained in this decentralized setting, namely through the matching of privacy preferences, set by the user, with requests for data and whether this can signify informed consent. Finally, we discuss the technical challenges of an implementation that caters to the previously identified legal requirements.