bluuebunny/crossref_metadata_2025 huggingface dataset:
In-Vitro and In-Silico Evaluations of Heterocyclic-Containing Diarylpentanoids as Bcl-2 Inhibitors Against LoVo Colorectal Cancer Cells
Authors: Leong Sze Wei , Chia Suet Lin , Abas Faridah , Yusoff Khatijah
Published: August 2020

In the present study, we investigated the in-vitro anti-cancer potential of six diarylpentanoids against a panel of BRAF- and KRAS-mutated colorectal cancer cell lines including T84, SW620, LoVo, HT29, NCI-H508, RKO, and LS411N cells. Structure–activity relationship study suggested that the insertions of tetrahydro-4H-thiopyran-4-one and brominated phenyl moieties are essential for better cytotoxicity. Among the evaluated analogs, 2e has been identified as the lead compound due to its low IC50 values of approximately 1 µM across all cancer cell lines and high chemotherapeutic index of 7.1. Anti-proliferative studies on LoVo cells showed that 2e could inhibit cell proliferation and colony formations by inducing G2/M cell cycle arrest. Subsequent cell apoptosis assay confirmed that 2e is a Bcl-2 inhibitor that could induce intrinsic cell apoptosis by creating a cellular redox imbalance through its direct inhibition on the Bcl-2 protein. Further molecular docking studies revealed that the bromophenyl moieties of 2e could interact with the Bcl-2 surface pocket through hydrophobic interaction, while the tetrahydro-4H-thiopyran-4-one fragment could form additional Pi-sulfur and Pi-alkyl interactions in the same binding site. In all, the present results suggest that 2e could be a potent lead that deserves further modification and investigation in the development of a new Bcl-2 inhibitor.
Carbohydrates Profile, Polyphenols Content and Antioxidative Properties of Beer Worts Produced with Different Dark Malts Varieties or Roasted Barley Grains
Authors: Gąsior Justyna , Kawa-Rygielska Joanna , Kucharska Alicja Z.
Published: August 2020

The aim of this study was to assess the possibility of shaping properties of beers at the stage of brewing wort production with the use of various types of special malts (chocolate pale, chocolate dark, wheat chocolate, brown barley) and roasted barley grains. The carbohydrate profile, polyphenols content, antioxidant capacity, 5-hydroxymethylfurfural content, and the browning index level were analyzed. Statistical analysis showed significant differences in the values of the examined features between the samples. The sugars whose content was most affected by the addition of special malts were maltose and dextrins. The polyphenol content in worts with 10% of additive was 176.02–397.03 mg GAE/L, ferric reducing antioxidant power (FRAP) 1.32–2.07 mmol TE/L, and capacity to reduction radical generated from 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS•+) 1.46–2.70 mmol TE/L. Wort with 40% dark malt showed the highest content of polyphenolic compounds and antioxidant activity (FRAP and ABTS•+). The HMF content and the browning index value were higher for wort with the addition of darker-colored malts (EBC) and increased with increasing dark malt dose.
Anti-Melanogenesis Activity of 6-O-Isobutyrylbritannilactone from Inula britannica on B16F10 Melanocytes and In Vivo Zebrafish Models
Authors: Jang Dae Kil , Pham Chau Ha , Lee Ik Soo , Jung Seung-Hyun , Jeong Ji Hye , Shin Han-Seung , Yoo Hee Min
Published: August 2020

A potential natural melanogenesis inhibitor was discovered in the form of a sesquiterpene isolated from the flowers of Inula britannica, specifically 6-O-isobutyrylbritannilactone (IBL). We evaluated the antimelanogenesis effects of IBL on B16F10 melanocytes and zebrafish embryos. As a result, we found that 3-isobutyl-1-methylxanthine (IBMX)-induced melanin production was reduced in a dose-dependent manner in B16F10 cells by IBL. We also analyzed B16F10 cells that were and were not treated with IBMX, investigating the melanin concentration, tyrosinase activity, mRNA levels. We also studied the protein expressions of microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related proteins (TRP1, and TRP2). Furthermore, we found that melanin synthesis and tyrosinase expression were also inhibited by IBL through the modulation of the following signaling pathways: ERK, phosphoinositide 3-kinase (PI3K)/AKT, and CREB. In addition, we studied antimelanogenic activity using zebrafish embryos and found that the embryos had significantly reduced pigmentation in the IBL-treated specimens compared to the untreated controls.
Lanthanide Luminescence in Visible-Light-Promoted Photochemical Reactions
Authors: Barraza Ramiro , Allen Matthew
Published: August 2020

The excitation of lanthanides with visible light to promote photochemical reactions has garnered interest in recent years. Lanthanides serve as initiators for photochemical reactions because they exhibit visible-light-promoted 4f→5d transitions that lead to emissive states with electrochemical potentials that are more negative than the corresponding ground states. The lanthanides that have shown the most promising characteristics for visible-light promoted photoredox are SmII, EuII, and CeIII. By understanding the effects that ligands have on the 5d orbitals of SmII, EuII, and CeIII, luminescence and reactivity can be rationally modulated using coordination chemistry. This review briefly overviews the photochemical reactivity of SmII, EuII, and CeIII with visible light; the properties that influence the reactivity of these ions; and the research that has been reported towards modulating their photochemical-relevant properties using visible light and coordination chemistry.
Manipulation and Applications of Hotspots in Nanostructured Surfaces and Thin Films
Authors: Zhao Xiaoyu , Wen Jiahong , Zhu Aonan , Cheng Mingyu , Zhu Qi , Zhang Xiaolong , Wang Yaxin , Zhang Yongjun
Published: August 2020

The synthesis of nanostructured surfaces and thin films has potential applications in the field of plasmonics, including plasmon sensors, plasmon-enhanced molecular spectroscopy (PEMS), plasmon-mediated chemical reactions (PMCRs), and so on. In this article, we review various nanostructured surfaces and thin films obtained by the combination of nanosphere lithography (NSL) and physical vapor deposition. Plasmonic nanostructured surfaces and thin films can be fabricated by controlling the deposition process, etching time, transfer, fabrication routes, and their combination steps, which manipulate the formation, distribution, and evolution of hotspots. Based on these hotspots, PEMS and PMCRs can be achieved. This is especially significant for the early diagnosis of hepatocellular carcinoma (HCC) based on surface-enhanced Raman scattering (SERS) and controlling the growth locations of Ag nanoparticles (AgNPs) in nanostructured surfaces and thin films, which is expected to enhance the optical and sensing performance.
Constructing a Sr2+-Substituted Surface Hydroxyapatite Hexagon-Like Microarray on 3D-Plotted Hydroxyapatite Scaffold to Regulate Osteogenic Differentiation
Authors: Wei Yingqi , Gao Huichang , Hao Lijing , Shi Xuetao , Wang Yingjun
Published: August 2020

Surface topography and chemical characteristics can regulate stem cell proliferation and differentiation, and decrease the bone-healing time. However, the synergetic function of the surface structure and chemical cues in bone-regeneration repair was rarely studied. Herein, a strontium ion (Sr2+)-substituted surface hydroxyapatite (HA) hexagon-like microarray was successfully constructed on 3D-plotted HA porous scaffold through hydrothermal reaction to generate topography and chemical dual cues. The crystal phase of the Sr2+-substituted surface microarray was HA, while the lattice constant of the Sr2+-substituted microarray increased with increasing Sr2+-substituted amount. Sr2+-substituted microarray could achieve the sustainable release of Sr2+, which could effectively promote osteogenic differentiation of human adipose-derived stem cells (ADSCs) even without osteogenic-induced media. Osteogenic characteristics were optimally enhanced using the higher Sr2+-substituted surface microarray (8Sr-HA). Sr2+-substituted microarray on the scaffold surface could future improve the osteogenic performance of HA porous scaffold. These results indicated that the Sr2+-substituted HA surface hexagon-like microarray on 3D-plotted HA scaffolds had promising biological performance for bone-regeneration repair scaffold.
Phytochemistry, Traditional Use and Pharmacological Activity of Picrasma quassioides: A Critical Reviews
Authors: Mohd Jamil Muhammad Daniel Hakim , Taher Muhammad , Susanti Deny , Rahman Md Atiar , Zakaria Zainul Amiruddin
Published: August 2020

Picrasma quassioides is a member of the Simaroubaceae family commonly grown in the regions of Asia, the Himalayas, and India and has been used as a traditional herbal medicine to treat various illnesses such as fever, gastric discomfort, and pediculosis. This study aims to critically review the presence of phytochemicals in P. quassioides and correlate their pharmacological activities with the significance of its use as traditional medicine. Data were collected by reviewing numerous scientific articles from several journal databases on the pharmacological activities of P. quassioides using certain keywords. As a result, approximately 94 phytochemicals extracted from P. quassioides were found to be associated with quassinoids, β-carbolines and canthinones. These molecules exhibited various pharmacological benefits such as anti-inflammatory, antioxidant, anti-cancer, anti-microbial, and anti-parasitic activities which help to treat different diseases. However, P. quassioides were also found to have several toxicity effects in high doses, although the evidence regarding these effects is limited in proving its safe use and efficacy as herbal medicine. Accordingly, while it can be concluded that P. quassioides may have many potential pharmacological benefits with more phytochemistry discoveries, further research is required to determine its real value in terms of quality, safety, and efficacy of use.
Effects of Launaea sarmentosa Extract on Lipopolysaccharide-Induced Inflammation via Suppression of NF-κB/MAPK Signaling and Nrf2 Activation
Authors: Nguyen Thanh Q. C. , Binh Tran Duy , Kusunoki Ryo , Pham Tuan L. A. , Nguyen Yen D. H. , Nguyen Trong Tuan , Kanaori Kenji , Kamei Kaeko
Published: August 2020

Launaea sarmentosa has been extensively used as a nutrient herb in traditional Vietnamese remedies for the treatment of various diseases, especially inflammatory diseases. However, no detailed research has been conducted examining the molecular mechanisms involved in the suppression of inflammatory response. Here, we studied the effects of L. sarmentosa methanol extract on lipopolysaccharide (LPS)-induced inflammation using RAW 264.7 macrophages. The extract demonstrated potent antioxidant activity owing to the presence of polyphenolic and flavonoid components. Pretreatment with the extract inhibited LPS-mediated secretion of nitric oxide, reactive oxygen species, and tumor necrosis factor-α as well as the expression of inflammatory cytokines. Furthermore, the activation of the nuclear factor-kappa B pathway and phosphoinositide-3-kinase/protein kinase B pathways was blocked by the extract by inhibiting Akt phosphorylation. Additionally, the mitogen-activated protein kinase pathway was suppressed, and endoplasmic reticulum stress was attenuated. Furthermore, the extract promoted the activity of nuclear factor erythroid-2-related factor 2 resulting in the up-regulation of heme oxygenase-1 pathway, leading to the suppression of oxidative stress and inflammatory response. Taken together, the results indicate that L. sarmentosa exhibits anti-inflammatory effects, and hence, can be further developed as a novel drug for the treatment of diseases associated with excessive inflammation.
The Low Glutamate Diet Effectively Improves Pain and Other Symptoms of Gulf War Illness
Authors: Holton Kathleen F. , Kirkland Anna E. , Baron Michael , Ramachandra Shalini S. , Langan Mackenzie T. , Brandley Elizabeth T. , Baraniuk James N.
Published: August 2020

Gulf War Illness (GWI) is a multisymptom disorder including widespread chronic pain, fatigue and gastrointestinal problems. The objective of this study was to examine the low glutamate diet as a treatment for GWI. Forty veterans with GWI were recruited from across the US. Outcomes included symptom score, myalgic score, tender point count, dolorimetry and the Chalder Fatigue Scale. Subjects were randomized to the low glutamate diet or a wait-listed control group, with symptom score being compared after one month. Subjects then went onto a double-blind, placebo-controlled crossover challenge with monosodium glutamate (MSG)/placebo to test for return of symptoms. Symptom score was compared between diet intervention and wait-listed controls with an independent t-test and effect size was calculated with Cohen’s d. Change scores were analyzed with Wilcoxon Signed Rank tests. Crossover challenge results were analyzed with General Linear Models and cluster analysis. The diet intervention group reported significantly less symptoms (p = 0.0009) than wait-listed controls, with a very large effect size, d = 1.16. Significant improvements in average dolorimetry (p = 0.0006), symptom score, tender point number, myalgic score and the Chalder Fatigue Scale (all p < 0.0001) were observed after the 1-month diet. Challenge with MSG/placebo resulted in significant variability in individual response. These results suggest that the low glutamate diet can effectively reduce overall symptoms, pain and fatigue in GWI, but differential results upon challenge suggest that other aspects of the diet, or underlying differences within the population, may be driving these changes. Future research is needed to identify potential nutrient effects, biomarkers, and underlying metabolic differences between responders and non-responders.
High Prevalence of Supplement Intake with a Concomitant Low Information Quality among Swiss Fitness Center Users
Authors: Mettler Samuel , Bosshard Joëlle Vera , Häring Dino , Morgan Gareth
Published: August 2020

Background: The aim of this study was to screen the prevalence of supplement use in Swiss fitness center users and what information sources they consulted. Methods: Customers of 10 fitness centers were screened with a quantitative questionnaire. Results: Eighty two percent of the 417 fitness center users consumed at least one supplement per week. Supplement intake correlated with training frequency (rs = 0.253, p < 0.001). The most prevalent products were protein supplements (used by 49% of the study population), magnesium (34%), and multi-micronutrient supplements (31%). The average number of supplement servings per week among consumers was 17.1 (SD: 16.1, median: 11.0) and the average number of different products used was 6.9 (SD: 4.4, median: 6.0). The most frequently used information sources were the coach/trainer (28%), the website of the supplement seller (26%), and training peers (24%). Thirty seven percent were informed or informed themselves about potential risks associated with the supplement used. The leading reasons for selecting the information source were the desire for scientific-based information followed by the education level of the informing person. Conclusions: A high prevalence of supplement intake among Swiss fitness center users was associated with a low level of information quality and a low prevalence of risk information. A discrepancy between a desire for high quality evidence-based information and a contrasting behavior was detected.
The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin
Authors: Bostan Marinela , Petrică-Matei Georgiana Gabriela , Radu Nicoleta , Hainarosie Razvan , Stefanescu Cristian Dragos , Diaconu Carmen Cristina , Roman Viviana
Published: August 2020

Natural compounds can modulate all three major phases of carcinogenesis. The role of the natural compounds such as resveratrol (RSV) and curcumin (CRM) in modulation of anticancer potential of platinum-based drugs (CisPt) is still a topic of considerable debate. In order to enhance head and neck cancer (HNSCC) cells’ sensitivity to the cytotoxic effects of CisPt combined treatments with RSV or CRM were used. The study aim was to evaluate how the RSV or CRM associated to CisPt treatment modulated some cellular processes such as proliferation, P21 gene expression, apoptotic process, and cell cycle development in HNSCC tumor cell line (PE/CA-PJ49) compared to a normal cell line (HUVEC). The results showed that RSV or CRM treatment affected the viability of tumor cells more than normal cells. These natural compounds act against proliferation and sustain the effects of cisplatin by cell cycle arrest, induction of apoptosis and amplification of P21 expression in tumor cells. In conclusion, using RSV or CRM as adjuvants in CisPt therapy might have a beneficial effect by supporting the effects induced by CisPt.
A Fluorinated Analogue of Marine Bisindole Alkaloid 2,2-Bis(6-bromo-1H-indol-3-yl)ethanamine as Potential Anti-Biofilm Agent and Antibiotic Adjuvant Against Staphylococcus aureus
Authors: Campana Raffaella , Mangiaterra Gianmarco , Tiboni Mattia , Frangipani Emanuela , Biavasco Francesca , Lucarini Simone , Citterio Barbara
Published: August 2020

Methicillin resistant Staphylococcus aureus (MRSA) infections represent a major global healthcare problem. Therapeutic options are often limited by the ability of MRSA strains to grow as biofilms on medical devices, where antibiotic persistence and resistance is positively selected, leading to recurrent and chronic implant-associated infections. One strategy to circumvent these problems is the co-administration of adjuvants, which may prolong the efficacy of antibiotic treatments, by broadening their spectrum and lowering the required dosage. The marine bisindole alkaloid 2,2-bis(6-bromo-1H-indol-3-yl)ethanamine (1) and its fluorinated analogue (2) were tested for their potential use as antibiotic adjuvants and antibiofilm agents against S. aureus CH 10850 (MRSA) and S. aureus ATCC 29213 (MSSA). Both compounds showed antimicrobial activity and bisindole 2 enabled 256-fold reduction (ΣFICs = 0.5) in the minimum inhibitory concentration (MIC) of oxacillin for the clinical MRSA strain. In addition, these molecules inhibited biofilm formation of S. aureus strains, and compound 2 showed greater eradicating activity on preformed biofilm compared to 1. None of the tested molecules exerted a viable but non-culturable cells (VBNC) inducing effect at their MIC values. Moreover, both compounds exhibited no hemolytic activity and a good stability in plasma, indicating a non-toxic profile, hence, in particular compound 2, a potential for in vivo applications to restore antibiotic treatment against MRSA infections.
Output Power Monitoring of Ultraviolet Light-Emitting Diode via Sapphire Substrate
Authors: Chen Ching-Hua , Zhang Jia-Jun , Wang Chang-Han , Chou Tzu-Chieh , Chan Rui-Xiang , Yeh Pinghui S.
Published: August 2020

Ultraviolet (UV) light plays an important role in air/water/surface sterilization now. Maintaining a certain light intensity is often required to attain the targeted effect. In this paper, on-chip power monitoring of a UV-A light-emitting diode (LED) via sapphire substrate is reported. A p–i–n photodiode loop that surrounds the UV-A LED was designed and fabricated to monitor the output power by detecting the scattered light of the LED propagating through the sapphire substrate. No particular waveguide structure or processing parameter control was needed. The monitoring responsivities per unit of surface-emitting power obtained were approximately 21 and 25 mA/W at photodiode biases of 0 and 3 V, respectively. When combined with a transimpedance amplifier, a monitoring responsivity of 1.87 V/mW at zero bias was measured, and a different monitoring responsivity could be customized by adjusting the gain of the transimpedance amplifier. The operation principle of this device might be applicable to UV-B or UV-C LEDs.
Composites with Natural Fibers and Conventional Materials Applied in a Hard Armor: A Comparison
Authors: Luz Fernanda , Garcia Filho Fabio , Oliveira Michelle , Nascimento Lucio , Monteiro Sergio
Published: August 2020

Natural-fiber-reinforced polymer composites have recently drawn attention as new materials for ballistic armor due to sustainability benefits and lower cost as compared to conventional synthetic fibers, such as aramid and ultra-high-molecular-weight polyethylene (UHMWPE). In the present work, a comparison was carried out between the ballistic performance of UHMWPE composite, commercially known as Dyneema, and epoxy composite reinforced with 30 vol % natural fibers extracted from pineapple leaves (PALF) in a hard armor system. This hard armor system aims to provide additional protection to conventional level IIIA ballistic armor vests, made with Kevlar, by introducing the PALF composite plate, effectively changing the ballistic armor into level III. This level of protection allows the ballistic armor to be safely subjected to higher impact projectiles, such as 7.62 mm caliber rifle ammunition. The results indicate that a hard armor with a ceramic front followed by the PALF/epoxy composite meets the National Institute of Justice (NIJ) international standard for level III protection and performs comparably to that of the Dyneema plate, commonly used in armor vests.
Preparation of Cross-Linked Graphene Oxide on Polyethersulfone Membrane for Pharmaceuticals and Personal Care Products Removal
Authors: Lou Yanyan , Tan Fibor J. , Zeng Rong , Wang Mengen , Li Pan , Xia Shengji
Published: August 2020

The unique two-dimensional structure and chemical properties of graphene oxide (GO) provide a convenient method for preparing novel membranes. In this study, GO membranes were prepared through filtration by a pressure-assisted self-assembly method involving the cross-linking of three diamine monomers on a polyethersulfone (PES) support. The different small molecular diamines, ethylenediamine, butanediamine, and p-phenylenediamine, were introduced as cross-linking agents to investigate the effect of diamine on the properties of GO membranes. The hydrophobic substances ibuprofen, gemfibrozil, and triclosan were selected as target pharmaceuticals and personal care products (PPCPs). The adsorption and molecular sieving activities of PPCPs by cross-linked GO membranes at a pH of 3 were investigated. The permeate water was analyzed for dissolved organic carbon, ultraviolet absorption at 254 nm, molecular weight distribution, and fluorescence excitation–emission matrices. The results showed that the removal of hydrophobic PPCPs by GO membranes was mainly due to their adsorption and molecular sieving activities. Adsorption was mainly determined by the hydrophilic and hydrophobic properties of the membranes and PPCPs. The interception effect was mainly determined by the interlayer spacing between the GO membranes and the molecular weight and steric hindrance of the PPCPs. A smaller spacing of the GO membrane layers resulted in greater steric hindrance and a higher removal rate.
Sustainable Development and Performance Evaluation of Marble-Waste-Based Geopolymer Concrete
Authors: Lee Wei-Hao , Lin Kae-Long , Chang Ting-Hsuan , Ding Yung-Chin , Cheng Ta-Wui
Published: August 2020

The key objective of this study was to develop marble-based geopolymer concrete and examine the viability of its application as a sustainable structural material for the construction industry. The results of the research demonstrated that marble-based geopolymer concrete can be developed, and its physical/mechanical properties were shown to have a very good performance. According to various experimental tests and a large-scale ready-mixed plant test, it was found that the marble-based geopolymer concrete displayed a good workability and was not easily influenced by temperature changes. The results showed that marble-based geopolymer concrete has an excellent potential for further engineering development in the future.
Functional Properties of Biopolymer-Based Films Modified with Surfactants: A Brief Review
Authors: Shamsuri Ahmad Adlie , Md. Jamil Siti Nurul Ain
Published: August 2020

An increase of environmental awareness recently has increased the interest of researchers in using of biopolymer-based films. The films have been prepared extensively by utilizing starch, carboxymethyl cellulose, chitosan, protein, gelatin, carrageenan, alginate, pectin, guar gum and pullulan. They are typically modified with surface-active agents (surfactants) such as glycerol monostearate, sucrose ester, sodium stearoyl lactate, sodium dodecyl sulfate, ethyl lauroyl arginate HCl, Span 20 to 80, Tween-20 to 80 and soy lecithin for improving the functional properties of the films. In this brief review, two types of biopolymer-based films that prepared through casting method were categorized, specifically solution- and emulsion-based films. The four types of surfactants, namely non-ionic, anionic, cationic and amphoteric surfactants that are regularly used to modify biopolymer-based films are also described. The functional properties of the films modified with different types of surfactants are briefly reviewed. This study enhances the attraction of researchers in biopolymer-based films and the improvement of new concepts in this niche area.
Research on Improved Intelligent Control Processes Based on Three Kinds of Artificial Intelligence
Authors: Liu Jingwei , Li Tianyue , Chen Jiaming , Zuo Fangling
Published: August 2020

Autotuning and online tuning of control parameters in control processes (OTP) are widely used in practice, such as in chemical production and industrial control processes. Better performance (such as dynamic speed and steady-state error) and less repeated manual-tuning workloads in bad environments for engineers are expected. The main works are as follows: Firstly, a change ratio for expert system and fuzzy-reasoning-based OTP methods is proposed. Secondly, a wavelet neural-network-based OTP method is proposed. Thirdly, comparative simulations are implemented in order to verify the performance. Finally, the stability of the proposed methods is analyzed based on the theory of stability. Results and effects are as follows: Firstly, the proposed control parameters of online tuning methods of artificial-intelligence-based classical control (AI-CC) systems had better performance, such as faster speed and smaller error. Secondly, stability was verified theoretically, so the proposed method could be applied with a guarantee. Thirdly, a lot of repeated and unsafe manual-based tuning work for engineers can be replaced by AI-CC systems. Finally, an upgrade solution AI-CC, with low cost, is provided for a large number of existing classical control systems.
Diurnal Differences in Human Muscle Isometric Force In Vivo Are Associated with Differential Phosphorylation of Sarcomeric M-Band Proteins
Authors: Ab Malik Zulezwan , Bowden Davies Kelly A. , Hall Elliott C. R. , Barrett Jennifer , Pullinger Samuel A. , Erskine Robert M. , Shepherd Sam O. , Iqbal Zafar , Edwards Ben J. , Burniston Jatin G.
Published: August 2020

We investigated whether diurnal differences in muscle force output are associated with the post-translational state of muscle proteins. Ten physically active men (mean ± SD; age 26.7 ± 3.7 y) performed experimental sessions in the morning (08:00 h) and evening (17:00 h), which were counterbalanced in order of administration and separated by at least 72 h. Knee extensor maximal voluntary isometric contraction (MVIC) force and peak rate of force development (RFD) were measured, and samples of vastus lateralis were collected immediately after exercise. MVIC force was greater in the evening (mean difference of 67 N, 10.2%; p < 0.05). Two-dimensional (2D) gel analysis encompassed 122 proteoforms and discovered 6 significant (p < 0.05; false discovery rate [FDR] = 10%) diurnal differences. Phosphopeptide analysis identified 1693 phosphopeptides and detected 140 phosphopeptides from 104 proteins that were more (p < 0.05, FDR = 22%) phosphorylated in the morning. Myomesin 2, muscle creatine kinase, and the C-terminus of titin exhibited the most robust (FDR < 10%) diurnal differences. Exercise in the morning, compared to the evening, coincided with a greater phosphorylation of M-band-associated proteins in human muscle. These protein modifications may alter the M-band structure and disrupt force transmission, thus potentially explaining the lower force output in the morning.
SARS-CoV-2 Translocate from Nasopharyngeal to Bronchoalveolar Site: A Case Presentation
Authors: Luciani Filippo , Cione Erika , Caroleo Maria Cristina , Colosimo Manuela , Zanolini Alfredo , Barca Antonio , Cosimo Stefano , Pasqua Pino , Gallelli Luca
Published: August 2020

The nasopharyngeal swab is commonly used for the diagnosis of SARS-CoV-2 infection. Since the swab is performed in this site, of course, it cannot detect the presence of the virus in other tissue districts such as the lung, brain, or bowel. In the present case report, the nasopharyngeal swab was negative twice. From this, the patient discontinued antiviral therapy. Nasopharyngeal swabs were maintained negative until five days later, when we recorded a severe impairment of the patient’s clinical condition. At this time, the bronchoalveolar lavage was positive for SARS-CoV-2. The purpose of the case herein described is to suggest paying attention to the nasopharyngeal swab result. A negative detection in nasopharyngeal swab could not be indicative of COVID-19 recovery.
Comparative Analysis of Summer Upwelling and Downwelling Events in NW Spain: A Model-Observations Approach
Authors: Lorente Pablo , Piedracoba Silvia , Montero Pedro , Sotillo Marcos G. , Ruiz María Isabel , Álvarez-Fanjul Enrique
Published: August 2020

Upwelling and downwelling processes play a critical role in the connectivity between offshore waters and coastal ecosystems, having relevant implications in terms of intense biogeochemical activity and global fisheries production. A variety of in situ and remote-sensing networks were used in concert with the Iberia–Biscay–Ireland (IBI) circulation forecast system, in order to investigate two persistent upwelling and downwelling events that occurred in the Northwestern (NW) Iberian coastal system during summer 2014. Special emphasis was placed on quality-controlled surface currents provided by a high-frequency radar (HFR), since this land-based technology can effectively monitor the upper layer flow over broad coastal areas in near-real time. The low-frequency spatiotemporal response of the ocean was explored in terms of wind-induced currents’ structures and immediacy of reaction. Mean kinetic energy, divergence and vorticity maps were also calculated for upwelling and downwelling favorable events, in order to verify HFR and IBI capabilities, to accurately resolve the prevailing surface circulation features, such as the locus of a persistent upwelling maximum in the vicinity of Cape Finisterre. This integrated approach proved to be well-founded to efficiently portray the three-dimensional characteristics of the NW Iberian coastal upwelling system regardless of few shortcomings detected in IBI performance, such as the misrepresentation of the most energetic surface dynamics or the overestimation of the cooling and warming associated with upwelling and downwelling conditions, respectively. Finally, the variability of the NW Iberian upwelling system was characterized by means of the development of a novel ocean-based coastal upwelling index (UI), constructed from HFR-derived hourly surface current observations (UIHFR). The proposed UIHFR was validated against two traditional UIs for 2014, to assess its credibility. Results suggest that UIHFR was able to adequately categorize and characterize a wealth of summer upwelling and downwelling events of diverse length and strength, paving the way for future investigations of the subsequent biophysical implications.
Discrepancies in the Simulated Global Terrestrial Latent Heat Flux from GLASS and MERRA-2 Surface Net Radiation Products
Authors: Guo Xiaozheng , Yao Yunjun , Zhang Yuhu , Lin Yi , Jiang Bo , Jia Kun , Zhang Xiaotong , Xie Xianhong , Zhang Lilin , Shang Ke , Yang Junming , Bei Xiangyi
Published: August 2020

Surface all-wave net radiation (Rn) is a crucial variable driving many terrestrial latent heat (LE) models that estimate global LE. However, the differences between different Rn products and their impact on global LE estimates still remain unclear. In this study, we evaluated two Rn products, Global LAnd Surface Satellite (GLASS) beta version Rn and Modern-Era Retrospective Analysis for Research and Applications-version 2 (MERRA-2) Rn, from 2007–2017 using ground-measured data from 240 globally distributed in-situ radiation measurements provided by FLUXNET projects. The GLASS Rn product had higher accuracy (R2 increased by 0.04–0.26, and RMSE decreased by 2–13.3 W/m2) than the MERRA-2 Rn product for all land cover types on a daily scale, and the two Rn products differed greatly in spatial distribution and variations. We then determined the resulting discrepancies in simulated annual global LE using a simple averaging model by merging five diagnostic LE models: RS-PM model, SW model, PT-JPL model, MS-PT model, and SIM model. The validation results showed that the estimated LE from the GLASS Rn had higher accuracy (R2 increased by 0.04–0.14, and RMSE decreased by 3–8.4 W/m2) than that from the MERRA-2 Rn for different land cover types at daily scale. Importantly, the mean annual global terrestrial LE from GLASS Rn was 2.1% lower than that from the MERRA-2 Rn. Our study showed that large differences in satellite and reanalysis Rn products could lead to substantial uncertainties in estimating global terrestrial LE.
A Semi-Empirical Chlorophyll-a Retrieval Algorithm Considering the Effects of Sun Glint, Bottom Reflectance, and Non-Algal Particles in the Optically Shallow Water Zones of Sanya Bay Using SPOT6 Data
Authors: Yu Yan , Chen Shengbo , Qin Wenhan , Lu Tianqi , Li Jian , Cao Yijing
Published: August 2020

Chlorophyll-a (Chl-a) concentration retrieval is essential for water quality monitoring, aquaculture, and guiding coastline infrastructure construction. Compared with common ocean color satellites, land observation satellites have the advantage of a higher resolution and more data sources for retrieving the concentration of Chl-a from optically shallow waters. However, the sun glint (Rsg), bottom reflectance (Rb), and non-algal particle (NAP) derived from terrigenous matter affect the accuracy of Chl-a concentration retrieval using land observation satellite image data. In this paper, we propose a semi-empirical algorithm based on the remote sensing reflectance (Rrs) of SPOT6 to retrieve the Chl-a concentration in Sanya Bay (SYB), considering the effect of Rsg, Rb, and NAP. In this semi-empirical algorithm, the Cox–Munk anisotropic model and radiative transfer model (RTM) were used to reduce the effects of Rsg and Rb on Rrs, and the Chl-a concentration was retrieved by the Chl-a absorption coefficient at 490 nm (aphy(490)) to remove the effect of NAP. The semi-empirical algorithm was in the form of Chl-a = 43.3[aphy(490)]1.454, where aphy (490) was calculated by the total absorption coefficient and the absorption coefficients of each component by empirical algorithms. The results of the Chl-a concentration retrieval show the following: (1) SPOT6 data are available for Chl-a retrieval using this semi-empirical algorithm in oligotrophic or mesotrophic coastal waters, and the accuracy of the algorithm can be improved by removing the effects of Rsg, Rb, and NAP (R2 from 0.71 to 0.93 and root mean square error (RMSE) from 0.23 to 0.11 ug/L); (2) empirical algorithms based on the blue-green band are suitable for oligotrophic or mesotrophic coastal waters, and the algorithm based on the blue-green band difference Chl-a index (DCI) has stronger anti-interference in terms of the effects of sun glint and bottom reflectance than the algorithm based on the blue-green ratio (BGr); (3) in the case of ignoring Rsg unrelated to inherent optical properties (IOPs), NAP is the biggest interference factor when >9.5 mg/L and the effect of bottom reflectance should be considered when the water depth (H) <5 m in SYB; and (4) the inherent optical properties of the waters in SYB are dominated by NAP (Chl-a = 0.2–2.6 ug/L and NAP = 2.2–30.1 mg/L), and the nutrients are concentrated by enclosed terrain and southeast current. This semi-empirical algorithm for Chl-a concentration retrieval has the potential to monitor Chl-a in oligotrophic and mesotrophic coastal waters using other land observation satellites (e.g., Landsat8 OLI, ASTER, and GaoFen2).
Determination of Lidar Ratio for Major Aerosol Types over Western North Pacific Based on Long-Term MPLNET Data
Authors: Wang Sheng-Hsiang , Lei Heng-Wai , Pani Shantanu Kumar , Huang Hsiang-Yu , Lin Neng-Huei , Welton Ellsworth J. , Chang Shuenn-Chin , Wang Yueh-Chen
Published: August 2020

East Asia is the most complex region in the world for aerosol studies, as it encounters a lot of varieties of aerosols, and aerosol classification can be a challenge in this region. In the present study, we focused on the relationship between aerosol types and aerosol optical properties. We analyzed the long-term (2005–2012) data of vertical profiles of aerosol extinction coefficients, lidar ratio (Sp), and other aerosol optical properties obtained from a NASA Micro-Pulse Lidar Network and Aerosol Robotic Network site in northern Taiwan, which frequently receives Asian continental outflows. Based on aerosol extinction vertical profiles, the profiles were classified into two types: type 1 (single-layer structure) and type 2 (two-layer structure). Fall season (October–November) was the prevailing season for the Type 1, whereas type 2 mainly happened in spring (March–April). In type 1, air masses normally originated from three regional sectors, i.e., Asia continental (AC), Pacific Ocean (PO), and Southeast Asia (SA). The mean Sp values were 39 ± 17 sr, 30 ± 12 sr, and 38 ± 18 sr for the AC, PO, and SA sectors, respectively. The Sp results suggested that aerosols from the AC sector contained dust and anthropogenic particles, and aerosols from the PO sector were most likely sea salts. We further combined the EPA dust event database and backward trajectory analysis for type 2. Results showed that Sp was 41 ± 14 sr and 53 ± 21 sr for dust storm and biomass-burning events, respectively. The Sp for biomass-burning events in type 2 showed two peaks patterns. The first peak occurred within range of 30–50 sr corresponding to urban pollutant, and the second peak occurred within range of 60–80 sr in relation to biomass burning. Finally, our study summarized the Sp values for four major aerosol types over northern Taiwan, viz., urban (42 ± 18 sr), dust (34 ± 6 sr), biomass-burning (69 ± 12 sr), and oceanic (30 ± 12 sr). Our findings provide useful references for aerosol classification and air pollution identification over the western North Pacific.
Standoff Chemical Detection Using Laser Absorption Spectroscopy: A Review
Authors: Li Jinyi , Yu Ziwei , Du Zhenhui , Ji Yue , Liu Chang
Published: August 2020

Remote chemical detection in the atmosphere or some specific space has always been of great interest in many applications for environmental protection and safety. Laser absorption spectroscopy (LAS) is a highly desirable technology, benefiting from high measurement sensitivity, improved spectral selectivity or resolution, fast response and capability of good spatial resolution, multi-species and standoff detection with a non-cooperative target. Numerous LAS-based standoff detection techniques have seen rapid development recently and are reviewed herein, including differential absorption LiDAR, tunable laser absorption spectroscopy, laser photoacoustic spectroscopy, dual comb spectroscopy, laser heterodyne radiometry and active coherent laser absorption spectroscopy. An update of the current status of these various methods is presented, covering their principles, system compositions, features, developments and applications for standoff chemical detection over the last decade. In addition, a performance comparison together with the challenges and opportunities analysis is presented that describes the broad LAS-based techniques within the framework of remote sensing research and their directions of development for meeting potential practical use.
Areal Extent of Dust Emission Events and Source Geomorphology in Northern Africa from MSG-SEVIRI Data
Authors: Lavi Bekin Ofer , Crouvi Onn , Blumberg Dan G.
Published: August 2020

In this study we (1) mapped the areal extent of current dust sources over Northern Africa between 8°W–31°E and 22°N - Mediterranean coast; and (2) identified and characterized the geomorphic units and soil types that emit dust from these areas. We used the full resolution (3 km) data from the MSG-SEVIRI to map dust sources over a 2-year period between 2005–2006, and examined these regions with remotely sensed images and geomorphic and soil maps. A total of >2600 individual dust emission events were mapped; with frequency up to 34 events in the 2-year study period. The areal extent of dust emission sources exhibited a lognormal distribution with most sources ranging from 20 to 130 km2. Most dust events were singular and related to a variety of specific geomorphic units. Dust events that created hotspots were mostly located over playas and fluvial landforms, and to a lesser extent over sand dunes and anthropogenic affected regions. About 20% of dust hotspots were offset a few kilometers from clear geomorphic units. Quantitative analysis of emissions revealed that dust sourced from various geomorphic units, among them playas (12%) and fluvial systems (10%). The importance of sand dunes as dust-emission sources greatly differs between examined datasets (7% vs. 30%). Our study emphasizes the importance of scattered dust emission events that are not considered as hotspots, as these sources are usually neglected in dust emission modeling.
Sensitivity Analysis and Validation of Daytime and Nighttime Land Surface Temperature Retrievals from Landsat 8 Using Different Algorithms and Emissivity Models
Authors: Sekertekin Aliihsan , Bonafoni Stefania
Published: August 2020

Land Surface Temperature (LST) is a substantial element indicating the relationship between the atmosphere and the land. This study aims to examine the efficiency of different LST algorithms, namely, Single Channel Algorithm (SCA), Mono Window Algorithm (MWA), and Radiative Transfer Equation (RTE), using both daytime and nighttime Landsat 8 data and in-situ measurements. Although many researchers conducted validation studies of daytime LST retrieved from Landsat 8 data, none of them considered nighttime LST retrieval and validation because of the lack of Land Surface Emissivity (LSE) data in the nighttime. Thus, in this paper, we propose using a daytime LSE image, whose acquisition is close to nighttime Thermal Infrared (TIR) data (the difference ranges from one day to four days), as an input in the algorithm for the nighttime LST retrieval. In addition to evaluating the three LST methods, we also investigated the effect of six Normalized Difference Vegetation Index (NDVI)-based LSE models in this study. Furthermore, sensitivity analyses were carried out for both in-situ measurements and LST methods for satellite data. Simultaneous ground-based LST measurements were collected from Atmospheric Radiation Measurement (ARM) and Surface Radiation Budget Network (SURFRAD) stations, located at different rural environments of the United States. Concerning the in-situ sensitivity results, the effect on LST of the uncertainty of the downwelling and upwelling radiance was almost identical in daytime and nighttime. Instead, the uncertainty effect of the broadband emissivity in the nighttime was half of the daytime. Concerning the satellite observations, the sensitivity of the LST methods to LSE proved that the variation of the LST error was smaller than daytime. The accuracy of the LST retrieval methods for daytime Landsat 8 data varied between 2.17 K Root Mean Square Error (RMSE) and 5.47 K RMSE considering all LST methods and LSE models. MWA with two different LSE models presented the best results for the daytime. Concerning the nighttime accuracy of the LST retrieval, the RMSE value ranged from 0.94 K to 3.34 K. SCA showed the best results, but MWA and RTE also provided very high accuracy. Compared to daytime, all LST retrieval methods applied to nighttime data provided highly accurate results with the different LSE models and a lower bias with respect to in-situ measurements.
A Framework for User Adaptation and Profiling for Social Robotics in Rehabilitation
Authors: Martín Alejandro , Pulido José C. , González José C. , García-Olaya Ángel , Suárez Cristina
Published: August 2020

Physical rehabilitation therapies for children present a challenge, and its success—the improvement of the patient’s condition—depends on many factors, such as the patient’s attitude and motivation, the correct execution of the exercises prescribed by the specialist or his progressive recovery during the therapy. With the aim to increase the benefits of these therapies, social humanoid robots with a friendly aspect represent a promising tool not only to boost the interaction with the pediatric patient, but also to assist physicians in their work. To achieve both goals, it is essential to monitor in detail the patient’s condition, trying to generate user profile models which enhance the feedback with both the system and the specialist. This paper describes how the project NAOTherapist—a robotic architecture for rehabilitation with social robots—has been upgraded in order to include a monitoring system able to generate user profile models through the interaction with the patient, performing user-adapted therapies. Furthermore, the system has been improved by integrating a machine learning algorithm which recognizes the pose adopted by the patient and by adding a clinical reports generation system based on the QUEST metric.
Proposal-Based Visual Tracking Using Spatial Cascaded Transformed Region Proposal Network
Authors: Zhang Ximing , Luo Shujuan , Fan Xuewu
Published: August 2020

Region proposal network (RPN) based trackers employ the classification and regression block to generate the proposals, the proposal that contains the highest similarity score is formulated to be the groundtruth candidate of next frame. However, region proposal network based trackers cannot make the best of the features from different convolutional layers, and the original loss function cannot alleviate the data imbalance issue of the training procedure. We propose the Spatial Cascaded Transformed RPN to combine the RPN and STN (spatial transformer network) together, in order to successfully obtain the proposals of high quality, which can simultaneously improves the robustness. The STN can transfer the spatial transformed features though different stages, which extends the spatial representation capability of such networks handling complex scenarios such as scale variation and affine transformation. We break the restriction though an easy samples penalization loss (shrinkage loss) instead of smooth L1 function. Moreover, we perform the multi-cue proposals re-ranking to guarantee the accuracy of the proposed tracker. We extensively prove the effectiveness of our proposed method on the ablation studies of the tracking datasets, which include OTB-2015 (Object Tracking Benchmark 2015), VOT-2018 (Visual Object Tracking 2018), LaSOT (Large Scale Single Object Tracking), TrackingNet (A Large-Scale Dataset and Benchmark for Object Tracking in the Wild) and UAV123 (UAV Tracking Dataset).