bluuebunny/crossref_metadata_2025 huggingface dataset:
Short communication: Functional assessment of citral dietary supplementation on growth performance, intestinal parameters, and specific activity of the digestive enzymes of Sardinella brasiliensis reared in RAS
Authors: Michelotti Bruna Tomazetti , Owatari Marco Shizuo , Magnotti Caio , Klinger Ana Carolina Kohlrausch , Palma Ulyssses Silva , Santos Marina Cesconeto dos , Descovi Sharine Nunes , Cerqueira Vinicius Ronzani , Costa Sílvio Teixeira , Bianchin Letícia Bogoni , Baldisserotto Bernardo
Published: July 2023

This study evaluated the effects of dietary supplementation with citral on growth performance and intestinal parameters, and specific activity of the digestive enzymes of the Brazilian sardine Sardinella brasiliensis. The experiment was structured with two hundred and forty juvenile S. brasiliensis that were randomly distributed in a recirculating aquaculture system (RAS) composed of 12 circular tanks of 150 L (n = 20 per tank). A diet without citral addition (control) and three diets containing different levels of citral inclusion (0.5, 1.0 and 2.0 mL kg feed–1) were used in the fish feeding management during 20 days. After the period of dietary supplementation, the growth performance indexes of the fish were verified. Portions of the stomach and midgut were collected for analysis of digestive enzymes, as well as portions of the midgut for histological analysis. A significant positive relationship was found between citral supplementation and fish survival. In the specific activity of digestive enzymes, dietary citral supplementation at 2.0 mL kg feed–1 increased lipase and amylase activity. Citral supplementation positively influenced the number of villi and intestinal crypt depth. Furthermore, the inclusion of citral at 0.5 mL kg feed–1 increased the intestine diameter of the fish. However, the specific growth rate, weight gain and feed conversion were impaired with inclusions of citral at 0.5 and 1.0 mL kg feed–1. The results of the experiment suggest that the addition of citral as an additive in the diet of S. brasiliensis improved the survival of fish reared in RAS.
Ropivacaine-loaded hydrogels for prolonged relief of chemotherapy-induced peripheral neuropathic pain and potentiated chemotherapy
Authors: Qing Xin , Dou Renbin , Wang Peng , Zhou Mengni , Zhang Huiwen , Qiu Gaolin , Liu Hu , Yang Zhilai , Zhang Jiqian , Zhu Shasha , Liu Xuesheng
Published: July 2023

Chemotherapy can cause severe pain for patients, but there are currently no satisfactory methods of pain relief. Enhancing the efficacy of chemotherapy to reduce the side effects of high-dose chemotherapeutic drugs remains a major challenge. Moreover, the treatment of chemotherapy-induced peripheral neuropathic pain (CIPNP) is separate from chemotherapy in the clinical setting, causing inconvenience to cancer patients. In view of the many obstacles mentioned above, we developed a strategy to incorporate local anesthetic (LA) into a cisplatin-loaded PF127 hydrogel for painless potentiated chemotherapy. We found that multiple administrations of cisplatin-loaded PF127 hydrogels (PFC) evoked severe CIPNP, which correlated with increased pEKR-positive neurons in the dorsal root ganglion (DRG). However, incorporating ropivacaine into the PFC relieved PFC-induced CIPNP for more than ten hours and decreased the number of pEKR-positive neurons in the DRG. Moreover, incorporating ropivacaine into the PFC for chemotherapy is found to upregulate major histocompatibility complex class I (MHC-I) expression in tumor cells and promote the infiltration of cytotoxic T lymphocytes (CD8+ T cells) in tumors, thereby potentiating chemotherapy efficacy. This study proposes that LA can be used as an immunemodulator to enhance the effectiveness of chemotherapy, providing new ideas for painless cancer treatment.
Therapy-induced senescence drives immunotherapy resistance by inducing an immunosuppressive tumor microenvironment
Authors: Beauséjour Christian , Maggiorani Damien , Le Oanh , Lisi Véronique , Landais Séverine , Moquin-Beaudry Gaël , Lavallée Vincent-Philippe , Decaluwe Hélène
Published: July 2023

Immune checkpoint inhibitors (ICI) invigorate immune cell functions and are effective against many cancer types. However, the potential of ICI may be limited in situations where immune cell fitness is impaired. Here, we show that the efficacy of αPD-L1-based cancer immunotherapies is compromised by the accumulation of senescent cells in mice previously exposed to sublethal irradiation or injected with doxorubicin. Similarly, we observed that the efficacy of the combination of a αCTLA-4 antibody with radiotherapy is diminished in previously irradiated mice. In both models, resistance to immunotherapy was associated with a decrease in the accumulation and activation of CD8 T cells within tumors. Elimination of senescent cells by the injection of the senolytic drug ABT263, weeks before treatments, restored immune homeostasis within the tumor micro-environment (TME) and increased mice survival in both models. Using single-cell transcriptomic analysis, we observed that the injection of ABT263 reverses the exacerbated immunosuppressive profile of myeloid cells in the TME of previously irradiated mice. These myeloid cells, when purified from established tumors, were responsible for impaired CD8 T cell proliferation in vitro. Our study shows that cancer therapies, by inducing senescence, favor the formation of an immunosuppressive TME, which can alter the efficacy of ICI. The use of senolytic drugs before ICI may constitute an interesting pharmacological approach to improve the effectiveness of cancer immunotherapies.
Cyclosporine A enhanced the cytotoxicity of immunotoxin to the HER-2-overexpressing SK-OV-3 cells
Authors: Xie Huilong , fu yunrong , Lin Zhisheng , Wang Jiayi , Wu Jingyu , You Xiuhua , Xie Jieming
Published: July 2023

Background
Cyclosporine A (CsA) is a calcium antagonist mainly used as an immune-suppressive agent, which can enhance the cytotoxicity of immunotoxins through an unknown mechanism. In this study, T-CUS245C was an immunotoxin conjugated with trastuzumab(T) and recombinant cucurmosin 245C (CUS245C), commonly used to induce apoptosis and differentiation in caner cells.
Objectives
To explore the synergistic effect of T-CUS245C combined with CsA, and to reveal it's initial mechanism.
Methods
We investigated the synergistic effect of CsA combined with T-CUS245C on cell proliferation inhibition and apoptosis in SK-OV-3 cells with HER-2-overexpressing by SRB assay and apoptosis assays. The effect of CsA on the intracellular distribution of T-CUS245C was observed by confocal microscopy and quantified by flow cytometry.
Results
The results showed that the combination therapy index (CI) of CsA combined with T-CUS245C was less than 0.7, indicating that CsA could significantly enhance the proliferation inhibition and apoptosis induced by T-CUS245C in SK-OV-3 cells. The result of confocal microscopy and flow cytometry showed that CsA could effectively increase the dispersion of FITC-T-CUS245C in the cytoplasm, suggesting that CsA might enhance the cytotoxicity of T-CUS245C by promoting the endolysosomal escape.
Conclusion
CsA might improve the cytotoxicity of T-CUS245C by facilitating the release of immunotoxin in the intracellular space, which could be a potencial strategy to transport antibody-based drugs from endolysosomes into the cytoplasm.
A comparative analysis of core needle biopsy and repeat fine needle aspiration in patients with inconclusive initial cytology of thyroid Nodules
Authors: Su Xuejiao , Yue Can , Yang Wanting , Ma BuYun
Published: July 2023

Purpose To assess and compare the effectiveness of ultrasound-guided core needle biopsy (CNB) in comparison to repeat fine-needle aspiration(rFNA) for thyroid nodules that yield inconclusive results following the initial fine-needle aspiration (FNA).Methods A cohort of 471 patients who received an inconclusive cytological diagnosis following the initial FNA were included in this study. These patients subsequently underwent either CNB (n = 242) or rFNA (n = 229). The inconclusive FNA results encompassed categories I, III, and IV of the TBSRTC, as well as the ultrasound images indicating malignancy despite FNA results falling under TBSRTC category II. This study assessed the sampling satisfaction rate, diagnostic efficacy, and complications associated with CNB compared to rFNA. Additionally, the impact of repeat puncture time and nodule size on diagnostic efficacy was analyzed.Results Following repeat punctures, the satisfaction rate of the CNB sampling was found to be significantly higher than that of rFNA (83.9% vs 66.8%). The diagnostic rate in the CNB group was significantly greater compared to that of the rFNA group (71.1% vs 35.8%). In patients with nodule maximum diameters ranging from 5 mm to less than 8 mm, 8 mm to less than 10 mm, and 10 mm to less than 20 mm, the diagnostic accuracy was significantly higher in the CNB group compared to that in the rFNA group. In patients with intervals less than 90 days, between 90 and 180 days, and between 180 days and one year, the diagnostic rate in the CNB group was found to be higher compared to that in the rFNA group. In CNB ,not immediately adjacent to the capsule was a risk factor for nodular puncture bleeding (37.0% vs 22.7%.)Conclusion CNB demonstrated higher rates of satisfaction and diagnosis compared to the rFNA. The diagnostic effectiveness of CNB was not influenced by the time interval or the size of the thyroid nodule. Therefore, in cases where the initial FNA diagnosis of thyroid nodules is inconclusive, CNB should be considered as a viable option for re-puncture.
The energy consumption and efficiency of crop production in China
Authors: Mu Muxiaoya , Ge Xiaodong , Cao Congge , Zeng Yanyu , Xu Tianle , Huang Jiajia , Rong Weikang
Published: July 2023

Energy efficiency improvement is essential to the adaptiveness of agriculture to climate change in China, especially to meet the demands for both carbon neutrality and food security. This study examines the roles of agricultural inputs in energy consumption, crop production growth, and energy efficiency improvement from a long-term perspective (1949 to 2020). The results are as follows. (1) The total energy consumption of crop production increased at various increasing rates in different phases. (2) Energy efficiency presented a downward trend, from 0.468 tons of biomass per GJ energy consumption in 1949 to 0.178 tons per GJ in 2020. The decrease in energy efficiency slowed down when the agricultural biomass output increased. (3) From 1949 to 1978, mechanization and irrigation dominated the change in energy efficiency in crop production, accounting for about 47% of total energy consumption and 30.1% of crop production growth. From 1978 to 2012, fertilizer, pesticides, and film contributed 36% of total energy consumption and 6.0% of crop biomass growth. From 2012 to 2020, as fertilizer and pesticides were limited in crop production. Mechanization dominated the change in energy efficiency, accounting for about 18.7% of total energy consumption and 18.2% of crop production growth. (4) There were still challenges and uncertainties in agricultural energy efficiency improvement. The most important influencing factors include integrating energy efficiency and food security goals, rational allocation of the agricultural inputs, and further research and development of technologies for green agriculture innovation.
Research on the Z.S. technique for 8-0 polypropylene suture in scleral fixation of one-piece intraocular lens
Authors: Si Shancheng , Zhang Liu , Yang Rong , Li Yanhong , Zhang Jianhui
Published: July 2023

Purpose
To introduce a new scleral flapless knotless interlaminar anchoring technique (which we called Z.S. scleral fixation technique) for 8 − 0 polypropylene suture in fixation of intraocular lens (IOL) and evaluate its efficacy and safety.
SETTING:
Department of Glaucoma, Fuzhou Eye Hospital, 350007, Fuzhou, China
DESIGN:
Retrospective case series.
Methods
All surgeries were performed according to two strategies. At first, the Z.S. scleral fixation technique for 8 − 0 polypropylene suture in fixation of one-piece IOL was used to fix suture to sclera. And then, the one-piece IOL previously implanted in the anterior chamber was relocated to the posterior chamber. Finally, the suture ends outside the sclera were also ironed into the shape of a ball and buried in the sclera. All effectiveness and safety data related to the Z.S. technique was recorded and compared.
Results
The Z.S. scleral fixation technique was employed in 16 eyes of 13 patients, with the follow-up duration of at least 6 months. Postoperative uncorrected distance visual acuity (UDVA) was greatly improved in most of the operated eyes (11/16). The mean log MAR UDVA after surgery was significantly improved compared with that before surgery (0.93 ± 0.72 vs 1.53 ± 0.73, P = 0.002). IOLs in the vast majority of operated eyes (15/16) remained stable during half-year follow-up. No severe intra-/post- operative complications were observed in any of the operated eyes.
Conclusion
The Z.S. scleral fixation technique for 8 − 0 polypropylene suture in fixation of one-piece IOL reduced the surgical injuries and had ideal surgical efficacy and safety.
Central Attention with Multi-graphs for Image Annotation
Authors: Liu Baodi , Shao Qianqian , Liu Yan , Liu Weifeng
Published: July 2023

Automatic image annotation has exerted a tremendous fascination on researchers in recent decades due to the development of multimedia and computer vision. Much of the research only exercise the single graph to annotate the images in semi-supervised learning. And a multitude of methods examine how to use multi-view or image segmentation to compose multiple graph structures. However, the single graph is arduous to express the complete manifold structure information. Further, it is structurally computationally intensive and consumes a lot of time that building multiple graph structures depending on multiview or image segmentation. To this end, we propose a method, called Central Attention with Multi-graphs for Image Annotation. We firstly propose that the center part of an image is fatal for the image. And the promising performance can be accomplished relying solely on the two graph structures composed by central and overall features in semi-supervised learning. A number of experiments have been conducted on benchmark datasets, such as Corel5K, ESPGame, and IAPRTC12 to prove the effectiveness of the proposed method.
ACT001 inhibits tumor progression and reduces the expression of PD-L1 in non-small cell lung cancer
Authors: Li Yiman , Song Yan , Guo Hui , He Jinling , Hou Huijie , Shi Minmin , Shi Zhijing , Pan Fei , Ji Jinlu , Hu JunQi , Sun Zhijia , Chen Zhe
Published: July 2023

Purpose In recent years, remarkable advances in the therapeutic strategies for Non–small cell lung cancer (NSCLC) have revolutionized the treatment of NSCLC. However, in view of the low immune response, secondary drug resistance, autoimmune side effects, and financial burden of immunotherapy, developing novel immunotherapy or small-molecule compound is urged. Thus, this study investigates the effects of ACT001, a novel small-molecule inhibitor, on the antitumoral and immunomodulatory effects of ACT001 in NSCLC and explores the underlying mechanism.Methods The effects of ACT001 on NSCLC cells proliferation, clone formation, apoptosis, migration and invasion were measured using CCK8, clone formation, EDU stains, flow cytometry, transwell, and invasion assays, respectively. Flow cytometry was used to detect apoptosis of tumor cells and expression of granzyme B in CD3+ T cells in the co-culture system. Pull-down and mass spectrometry analysis were used to screen the interacting proteins of ACT001. Transcription factor binding sites were predicted by JASPAR database. Western blotting, rt-PCR, and flow cytometry were used to detect PD-L1, p-STAT1, and p-STAT3 expression in NSCLC cells exposed to ACT001.Results ACT001 inhibited the proliferation, migration, invasion and cell cycle progression of NSCLC cells, while promoted NSCLC cell apoptosis. ACT001 could decrease the expression of PD-L1 in NSCLC cells by inhibiting the phosphorylation of STAT1 and STAT3, which directly bind to the promoter of PD-L. Furthermore, ACT001 enhanced the release of granzyme B in CD3+ T cells, and promoted the apoptosis of NSCLC cells in the co-culture system.Conclusion We discovered that ACT001 exhibits a dual effect in NSCLC. On one hand, ACT001 has direct cytotoxicity on tumor cells in terms of inhibiting the proliferation, migration and invasion, and promoting NSCLC cell apoptosis. On the other hand, it also leads downregulation of PD-L1 in NSCLC cells to restore the anti-tumor ability of T cells. Our findings provide novel insights into the antitumoral and immunomodulatory effects of ACT001 which may shed new light on treatment of NSCLC.
Uncovering the mechanism of selective stabilization of high-energy stereoisomers via inclusion
Authors: Oakley Meagan , Oakes Madaline , Wagner Brian , Pearson Jason Kenneth
Published: July 2023

Supramolecular systems may be used to stabilize otherwise unstable isomers to find alternative synthetic pathways. It has been reported that cucurbit[8]uril can stabilize trans-I and trans-II CuIII cyclam whereas trans-III is the only non-substituted trans CuII cyclam stereoisomer found outside of the host molecule experimentally. Quantum chemical calculations can provide valuable insight into the intermolecular interactions involved in these inclusion complexes. All five possible trans stereoisomers of CuII cyclam were studied within the host to compute the interaction energy and free energy of association for each complex. The relative free energies of the five free cyclams confirm that trans-I and trans-II are the most energetically accessible stereoisomers from the initial trans-III starting point. Energy decomposition analysis was used to identify the attractive and repulsive interactions between cyclam and cucurbit[8]uril and showed that trans-II encounters repulsive forces almost three times greater than trans-I, which may explain the 7:3 ratio of trans-I to trans-II that occurs experimentally. Optimized complex geometries with trans-III, IV, and V show that the cyclams protrude out of cucurbit[8]uril, whereas trans-I and trans-II become more encapsulated and elongate the host, suggesting that the position of the cyclam is extremely important when forming non-covalent interactions. Our results agree with the experimental findings and provide greater insight into why the most stable isolated cyclam stereoisomer, trans-III, does not form a complex.
YY1-regulated AEBP1 drives the progression and inhibits ferroptosis of lung adenocarcinoma
Authors: Gao Qiang , Li Jie
Published: July 2023

Lung adenocarcinoma (LUAD) is the most common and severe type of lung cancer and it is characterized by high morbidity and mortality rates. Adipocyte enhancer binding protein 1 (AEBP1), a cancer-promoting factor in several types of cancer, plays a significant regulatory role in cancer. However, the regulatory mechanism underlying the effect of AEBP1 on LUAD has not been previously investigated. Therefore, in the present study the mRNA and protein expression levels of AEBP1 were detected in LUAD cells by reverse transcription-quantitative PCR and western blot analysis, respectively. Furthermore, AEBP1 was silenced in LUAD cells using the cell transfection technology and Cell Counting Kit-8, EdU, clonogenesis, wound healing, Transwell and tube formation assays were performed to assess cell proliferation, invasion, migration and angiogenesis. Subsequently, thiobarbituric acid-reactive substances production rate and Fe2+ levels were measured using the corresponding kits. Reactive oxygen species levels were detected by BODIPY (581/591) C11 staining, while the effect of AEBP1 silencing on ferroptosis was evaluated via detecting the expression levels of ferroptosis-related proteins by western blot analysis. The association between AEBP1 and Yin Yang 1 (YY1) was verified by luciferase reporter gene and chromatin immunoprecipitation assays. To further uncover the regulatory mechanism of AEBP1, cells were simultaneously transfected with AEBP1 knockdown and YY1overexpression plasmids and the relevant indicators of cell proliferation, invasion, migration, angiogenesis and ferroptosis were assessed. The results showed that AEBP1 silencing inhibited the proliferation, metastasis and angiogenesis of LUAD cells. Additionally, AEBP1 knockdown induced ferroptosis in LUAD cells. The above regulatory effects of AEBP1 were triggered via regulating YY1 transcription factor. Overall, the current study suggested that YY1-regulated AEBP1 could promote the progression of LUAD and inhibit ferroptosis.
Seismic Performance of 3Dimensional Interior Reinforced Concrete Beam-Column Joints Strengthened with CFRP sheets
Authors: Farahbod Farhang , Aalam Hamid , Jahanmohammadi Atefeh
Published: July 2023

Recent studies on flexural/shear strengthening of reinforced concrete beam-column joints with FRP composites have mainly focused on rehabilitation of 2Dimensional connections regardless of the effects of perpendicular beams. Present experimental study investigates the behavior of 3Dimensional interior RC joint under simultaneous lateral reversed cyclic loading in one direction and gravity loads in the orthogonal orientation. Three full-scale specimens were tested including one un-strengthened and two strengthened ones. Strengthening was performed using 1 and 3 L-shape layers of CFRP composite sheets at top and bottom faces of the joints, 1- and 4-strip wrapping layers at beams,1 and 2 full wrapping layers at column and conducting the method of externally bonded reinforcement on grooves (EBROG) to prohibit the premature FRP debonding. Results for failure mode, flexural capacity, ductility factor, energy dissipation and stiffness degradation indicate improvements in seismic behavior of flexural strengthened joints, where specially the increase in strength was reported 9 and 23 percents for two strengthened specimens. To evaluate the reported information, “sectional analysis theory” has been adopted for numerical prediction of maximum flexural capacity for 3D interior RC joints. Results are reliably compatible with the test results and observations as well. Comparing the reported values and numerical ones, the EBROG method appears successfully efficient in preventing FRP debonding and providing the maximum expected contribution of FRP in improving the seismic behavior.
Biodegradability and thermostability of renewable waterborne polyurethane synthesized from trihydroxyl poly(L-malic acid)/glycol based polyols and polycaprolactone diol
Authors: Yu Mingxuan , Song Xin , Niu Haibin , Zhou Chao , Liu Li , Wu Guangfeng
Published: July 2023

A biodegradable composite polyester-based waterborne polyurethane (L-AWPU) was synthesized by coupling L-malic acid and polyethylene glycol to obtain poly(L-malic acid ethylene glycol ester, PL-A) and polycaprolactone (PCL) as raw materials. The content of PL-A in polymer system was controlled to prepare L-AWPU with excellent biodegradable and thermostability properties. Compared to traditional PCL-WPU, the introduction of PL-A not only taking naturally degradable bio-based molecular chains into WPU, but also improving the branching degree of polyurethane molecular chains and formed intramolecular crosslinking structures due to the trifunctional hydroxyl group in malic acid. When the content of PL-A reached 20 wt%, the temperature of thermal weight loss (T5%) and maximum weight loss rate (Tmax) of polyurethane coating increased from 237.93°C and 259.91°C to 333.86°C and 343.13°C, respectively. The establishment of intramolecular crosslinking structures and increase in molecular weight improved the thermal stability of polyurethane. The tensile strength and elongation at break of L-AWPU showed no significant decrease compared to PCL-WPU at PL-A content of 20 wt%. The influence of PL-A content in polymer system on biodegradability of L-AWPU was specifically analyzed. The degradation rates of L-AWPU in 0.6% lipase PBS buffer solution and soil for 28 days reached 45.95% and 55.55% at PL-A content of 40 wt%, which were 1.71-fold and 1.52-fold increased compared to PCL-WPU. Containing natural bio-based molecular chains and trihydroxy crosslinked structures, L-AWPU showed excellent thermal stability and biodegradability, making it suitable for environmentally friendly and recyclable coatings.
Nano1D: An accurate Computer Vision model for segmentation and analysis of low-dimensional objects
Authors: Moradpur-Tari Ehsan , Vlassov Sergei , Oras Sven , Ernits Mart , Damerchi Elyad , Kyritsakis Andreas , Zadin Veronika
Published: July 2023

Microscopy images are usually analyzed qualitatively or manually and there is a need for autonomous quantitative analysis of objects. In this paper, we present a physics-based computational model for accurate segmentation and geometrical analysis of one-dimensional irregular and deformable objects from microscopy images. This model, named Nano1D, has four steps of preprocessing, segmentation, separating overlapped objects and geometrical measurements. The model is tested on Ag nanowires, and successfully segments and analyzes their geometrical characteristics including length, thickness, perimeter and distributions. The function of the algorithm is not undermined by the size, number, density, orientation and overlapping of objects in images. The main strength of the model is shown to be its ability to segment and analyze overlapping objects successfully with more than 99% accuracy, while current machine learning and computational models suffer from inaccuracy and inability to segment overlapping objects. Nano1D can analyze 1D nanoparticles including nanowires, nanotubes, nanorods in addition to other 1D features of microstructures like microcracks, dislocations etc.
The Cauchy Problem of the Three Dimensional Viscoelastic Flows with Initial Data Near Equilibrium
Authors: Fu Shengbin , Huang Wenting , Tian Fangfang
Published: July 2023

This paper explores the Cauchy problem of the three dimensional (3D) compressible viscoelastic fluids system of Oldroyd–B type. More precisely, by utilizing the temporal weighted energy method, we establish the global existence of strong solutions near equilibrium in the H3 (R3) space to this system, which is different from [Hu–Wu, SIAM J. Math. Anal., 45, 2013, 2815– 2833]. Besides, the main result in this paper provides the temporal decay rates of the solution.
Cannulated screw fixation assisted by a self-designed guide for the treatment of Garden stage I-III femoral neck fracture: A retrospective case-series study Running title: Self-designed guide for K-wire insertion
Authors: Li Jianwen , Wang Cheng , Wang Xiao , Chen Cheng , Jiang Jiantao
Published: July 2023

Purpose: To explore the effects of a self-designed Kirschner wire guiding and adjustment apparatus for internal fixation with cannulated screws for the treatment of femoral neck fracture.
Methods: We retrospectively reviewed the patients with femoral neck fracture (Garden I-III) treated at our hospital between 01/2018 and 3/2022. The cases were included in the present study according to the inclusion and exclusion criteria. The operation time, The times of provisional Kirschner wires(K-wires) insertion, times of fluoroscopy , estimated blood loss in operation, Harris score, and VAS score of the hip joint were recorded and analyzed.
Results: Forty patients were included (13 females and 17 males, (median, 58 years of age), with 20 patients in the unguided and 20 in the guided groups, respectively. Their body mass index (BMI) was 23.03±1.86 kg/m2. Compared with the unguided group, the guided group showed shorter operation time (median 0.20 vs. 0.43 h, P<0.001), less blood loss (median, 2 vs. 20 mL, P<0.001), less Insertion times of Kirschner wire (median, 7 vs. 15, P<0.001) and fluoroscopy times (median 12 vs. 20, P<0.001). The VAS score (median, 1vs. 1, P=0.359)and Harris score (92.75±4.83 vs. 89.75±5.20, P=0.066).shows no significant difference between groups at the final follow-up.
Conclusions: Using theself-designed Kirschner wire guiding and adjustment apparatus during fixation of femoral neck fracture with cannulated screws reduces the operation time, blood loss, insertion times of Kirschner wire and fluoroscopy. This might promote the recovery of patients. Those results should be confirmed in further studies.
Levels of Evidence: Level IV
Perfectionism in the anesthesiological environment
Authors: Kravets Olha , Yekhalov Vasyl , Stanin Dmytro , Krishtafor Daria , Martynenko Danylo
Published: July 2023

Background. Perfectionism today is understood as an individual's psychological conviction that the ideal can and should be achieved, and the imperfect result of work (physical, intellectual, etc.), in their opinion, has no right to exist.
The purpose of the study: Our goal was to consider this psychological phenomenon in the community of anesthesiology interns in comparison with the indicators of practicing doctors, to investigate its negative and positive features, their prevalence and dynamics.
Materials and methods. An anonymous survey of 92 medical interns and 124 participants of short-term trainings in the specialty "Anesthesiology" was conducted according to the M.M. Smith questionnaire. (2016).
Results. Interns showed an average level of tendency towards perfectionism on all other scales, except for "other-oriented perfectionism" where the level of the indicator was low. Practitioners showed an average level of tendency towards perfectionism on the scales "self-worth contingencies", "concern over mistakes", and "doubts about actions", according to all other indicators, the level of tendency was low. Among medical interns, the distribution on a Big-Three Perfectionism Scale showed that the degree of their attachment to rigid, self-critical, and narcissistic types of perfectionism was at an average level, including general. Practitioners showed an average level of tendency only to the rigid type of perfectionism, as for the self-critical and narcissistic types, the level was low, as well as the general one, which except for the average tendency to rigid perfectionism can be assessed as hypoperfectionism.
Conclusions. 1. Neither interns nor participants in training in the specialty "Anesthesiology" in general are supporters of hyper-perfectionism. 2. Intern doctors showed a rather high tendency to "concern over mistakes", which is explained by the fact that they are not yet specialists, but only learning. This can be assessed as constructive perfectionism. 3. Practitioners are prone to "self-oriented perfectionism", which involves high self-demand. This is explained by the specificity of the work of anesthesiologists (surgeons work in a team, an anesthesiologist - alone), who must first of all rely on themselves, and only then on the help of others. 4. Hypoperfectionism of the self-critical and narcissistic types in anesthesiologists with a certain length of work is associated with psychoprotective deviation of professional behavior, which excludes setting extremely high demands for the results of one's work, since the self-critical type implies a high probability of psycho-emotional burnout syndrome, and the narcissistic type sometimes leads to medical errors.
Symmetrization process and truncated orthogonal polynomials
Authors: Dominici Diego , García-Ardila Juan Carlos , Marcellán Francisco
Published: July 2023

We define the family of truncated Laguerre polynomials Pn(x; z), orthogonal with respect to the linear functional ℓ defined by ⟨ℓ, p⟩ = z 0 p(x)x α e −x dx, α > −1. The connection between Pn(x; z) and the polynomials Sn(x; z) (obtained through the symmetrization process) constitutes a key element in our analysis. As a consequence, several properties of the polynomials Pn(x; z) and Sn(x; z) are studied taking into account the relation between the parameters of the three-term recurrence relations that they satisfy. Asymptotic expansions of these coefficients are given. Discrete Painlevé and Painlevé equations associated with such coefficients appear in a natural way. An electro-static interpretation of the zeros of such polynomials as well as the dynamics of the zeros in terms of the parameter z are given.
MSC Classification: Primary: 42C05; 33C50
Metadichol® a Sirtuin Modulator for Anti-Aging Therapies
Authors: Raghavan P.R.
Published: July 2023

There are seven sirtuin genes in humans that encode seven sirtuin enzymes (SIRT1–7), each of which has unique functions and subcellular locations. Sirtuins are NAD+-dependent protein deacetylases that play a significant role in physiological processes such as energy metabolism, stress responses, DNA repair, and gene expression. Sirtuins are essential targets for aging-related diseases such as type 2 diabetes, inflammatory diseases, and neurodegenerative disorders. However, finding a single molecule that can activate all seven sirtuin genes is challenging because each isoform has a unique structure, substrates, and regulatory mechanisms. Most known sirtuin activators are specific for SIRT1, the most studied isoform of the sirtuin family. Here, we report that Metadichol®, a nano-emulsion of long-chain alcohols, induces 3- to 15-fold expression of all SIRT1–7 genes in human dermal fibroblasts when used in concentrations ranging from 1 pg/mL to 100 ng/mL. SIRT3 and FOXO1 gene expressions were 15-fold higher than those after treatment with Metadichol®. In addition, KL, FOXO1, TERT, and TP53 exhibited increased expression. Sirtuins and the four genes regulate aging, metabolism, and DNA repair and are age-related diseases like cancer, cardiovascular disease, and diabetes. All of these genes play essential roles in improving the quality of life as we age.
Identification of METTL3 as a myeloid-related prognosis biomarker in hepatocellular carcinoma using bioinformatics analysis and engineered mice model
Authors: Wang Qing , Wang He , Yang Changjie , Wang Xiao , Meng Jiaxiang
Published: July 2023

Background
Hepatocellular carcinoma (HCC) is a significant contributor to cancer-related mortality, ranking third in this regard. The epigenetic regulation of RNA N6-methyladenosine (m6A) modification in HCC has garnered considerable attention. This study utilized bioinformatics analysis and biologically engineered mice models to explore the immune and prognostic role of m6A modification in HCC.
Methods
We systematically analyzed genetic alterations, expression patterns, signaling pathways, prognostic features, and immunotherapy efficacy of the 21 m6A regulators in HCC as obtained from the Cancer Genome Atlas (TCGA), the Gene Expression Omnibus (GEO, GSE14520, GSE76427), and International Cancer Genome Consortium (IGCG) database; Unsupervised clustering, gene set variation analysis (GSVA), LASSO-COX regression, multivariate Cox regression, Nomogram, receiver operating characteristic (ROC) analysis, TIMER 2.0 and ImmuCellAI were used to perform the above analysis. Our analysis was verified with Mettl3F/FAlbumin-cre (liver-specific knockout, LKO) mice to establish a chemo-induced HCC model. The tumor immune microenvironment was analyzed with immunohistochemistry, immunofluorescence staining, and flow cytometry.
Results
The genetic alteration of the m6A modification gene set exhibited a correlation with reduced progression-free survival, diminished abundance of macrophage cells, and a lower score for immune cell infiltration. The cluster characterized by lower expression of the m6A gene set was linked to a more favorable overall survival (OS) and immune signaling, including IL2-STAT5, IL6-STAT3, IFN-gamma, and IFN-alpha signaling. Notably, the cluster with higher expression of m6A was associated with a higher homologous recombination deficiency (HRD) score and tumor mutational burden (TMB) score. Results of LASSO COX and the nomogram model underscored the significant contribution of METTL3 in the prognosis and ICB therapy of HCC. The results of Mettl3 LKO mice confirmed that Mettl3 LKO acted as a "rheostat" in the progression of HCC by regulating the mouse liver's myeloid-related innate and adaptive immune landscape.
Conclusions
In this study, we characterized the genetic, immune, and clinic landscape of the m6A gene set in HCC development and unveiled METTL3 as a molecular biomarker in epigenetic-related progress and ICB therapy of HCC from both informatics database analysis and engineered mice model.
Clinical Signature and Associated Immune Metabolism of NLRP1 in Pan-Cancer
Authors: Liao Yong , Yang Pinglian , Wang Jiaojiao , Liu Zhiping , Huang Zunnan
Published: July 2023

Scattered studies have shown the connection between NLRP1 which is responsible for inflammasome formation and tumor immunity as well as tumor metabolism; however, no research has yet systematically assessed the role and function of NLRP1 on various cancer types. Thus, in this study, data were retrieved and analyzed from public databases, and further showed that NLRP1 was differentially expressed in cancers. High NLRP1 expression was associated with a favorable prognosis for lung adenocarcinoma (LUAD) and pancreatic adenocarcinoma (PAAD). NLRP1 mutation status was also found to be associated with good prognosis. Further, NLRP1 expression was negatively related to tumor stemness, whereas positively related to immune infiltration of tumors. In addition, NLRP1 was found to be significantly related to tumor metabolism. A protein-protein interaction (PPI) network analysis was conducted for NLRP1, as well as pathways and functions of NLRP1. The study aimed to explore the role and function of NLRP1 in tumors by using pan-cancer analysis, and further suggested that NLRP1 may act as a promising therapeutic target for cancer.
Prediction of air quality index using auto machine learning tools over Andhra Pradesh state of India
Authors: Mogaraju Jagadish Kumar
Published: July 2023

Air quality is an essential aspect of any environmental study, and machine learning tools have provided a valuable method to predict the air quality index. In this study, Air Quality Index (AQI) is predicted from significant air quality variables like particulate matter-2.5 (PM 2.5), particulate matter-10 (PM10), NO, NO2, NOx, NH3, CO, SO2, O3, Benzene, Toluene, and xylene using autoML. Fourteen models were compared, and one was selected based on the significant model metrics. Random Forest Classifier is selected as an appropriate model based on model metrics: accuracy, AUC, Recall, Precision, F1 score, Kappa, and MCC. We obtained an accuracy score of 0.97 (97%) with good precision, recall, and F1. Our work supports that fine particulate matter (PM2.5) is crucial in predicting AQI. It is observed that the AutoML tools can be handy in machine learning tasks.
Students’ Need to Increase Ethically Acceptable Paraphrasing of Academic Texts
Authors: Rahmawan Andi Dian , Arwansyah Yanuar Bagas
Published: July 2023

The lack of Indonesian students performing their abilities in L1 paraphrasing becomes the primary reason they are labeled as persistent plagiarizers. Students, in Indonesian context and circumstances, need to broaden their perspectives regarding how to document the sources, using them in their original papers to support and defend the arguments. Literacy should be developed sustainably in order for students might critically think and state things with evidence. However, to say that students still developing English writing text as intentional plagiarism will appear to be overly harsh. Teachers, at the university level, should acknowledge that students’ commitment to plagiarism is the process of knowing its definition, characters, impacts, and habit of copying others’ work without any attempt to document it properly. Moreover, it also signs the teachers that students’ ability to use academic English needs improving. Observing these facts urges us to facilitate students to make them ready to conduct the paraphrase of the L2 texts while their EAP is still developing. This study seeks the fundamental preparation that should be made to generate acceptable paraphrasing texts.
Effects of water-saving irrigation planning on the underground water in the low area of Yellow River, China
Authors: Zhao Qian , Wang Jinhe , Wang Wei , Zhang Taiping , Wang Qiang , Chen Huanliang , Zhang Ruihua , Zhang Hongjun , Xu Yan , Gang Shenting , Li Changsuo , Xu Jingtao , Xu Zhipeng , Shi Yanfeng , Li Jingjing
Published: July 2023

The Yellow River Delta is considered one of the most water-stressed regions in China as the competition for water between various industrial sectors and agriculture is increasing. Since 2007, the regional governments have taken a leading role in agricultural water-saving planning by upgrading irrigation systems and constructing water-saving facilities. In this study, the effects of water-saving irrigation strategies on the groundwater table and quality were investigated in the Yellow River basin during the last two decades. The average phreatic water level of bore-wells was monitored as 13.47 m during 2011–2020 compared to the level of 12.09 m during 2011–2020. Besides, the depth of the deep confined aquifer dropped dramatically during 2001–2013 with a declining rate of 2.55 meters per annum (m/a) compared to the value of 0.385 m/a after 2014, suggesting the reduced exploitation of deep groundwater and increased availability of groundwater after the water-saving campaign. In addition, the physicochemical parameters of both phreatic water and deep confined aquifer indicated improved water quality. Consequently, understanding how the water-saving planning influences the groundwater availability is important, which would provide an insightful guidance for the water managers to protect the groundwater environment in the Yellow River Delta.
Patogenicidad de morfotipos endofíticos de Colletotrichum en hojas y ramas de mango (Mangifera indica l.) CV. azúcar, en el departamento del Magdalena, Colombia
Authors: Mercado Correa Alvaro José , Arrieta Mesa Luz Edith , Quintero Mercado Andrés Felipe , Páez Redondo Alberto Rafael
Published: July 2023

La antracnosis, asociada al género fitopatógeno Colletotrichum, es una de las enfermedades más limitantes de mango en el mundo al generar daños en campo y poscosecha. El patógeno es hemibiótrofo y además puede ser endófito asintomático; asimismo, algunas especies presentan quiescencia. El papel de estos estados biológicos como fuente de inóculo es aún incierto. Se evaluó la patogenicidad de 14 aislamientos endófitos-quiescentes de Colletotrichum obtenidos de estudios previos y de un aislamiento extraído de un fruto sintomático de mango cv. Azúcar. Al describir morfométricamente (colonia) los aislamientos en cultivos monospóricos y compararlos por su tasa de crecimiento, densidad de inóculo, conidias y apresorios, se encontraron diferencias estadísticas para las primeras tres variables. Asimismo, se evaluó la patogenicidad de los morfotipos en hojas y ramas de mango cv. Azúcar mediante inoculaciones con atomizador en árboles de dos años en condiciones de trópico seco, se describieron síntomas y se evaluaron variables biológicas (número, diámetro y tasa de crecimiento de lesiones). En hojas, todos los morfotipos indujeron lesiones, pero solo hubo diferencias en la tasa de crecimiento; en ramas, se observaron diferencias altamente significativas entre morfotipos en el número y diámetro de lesiones, y diferencias en la tasa de crecimiento de estas, aunque hubo morfotipos que no indujeron daños. Hay alta variabilidad morfológica y biológica entre morfotipos de Colletotrichum, y los aislamientos endofíticos son potencialmente infectivos sobre hojas y ramas de mango cv. Azúcar.
Variación fenológica de Rhizophora mangle, Conocarpus erectus y Laguncularia racemosa en Isla Múcura, Parque Nacional Natural Los Corales del Rosario y de San Bernardo, Caribe colombiano
Authors: Henao-Castro Alejandro , Valcárcel Castellanos Camilo , López Paternina Guido Ricardo
Published: July 2023

Los manglares son ecosistemas marino costeros estratégicos ofrecen una variedad de servicios a la humanidad. No obstante, son vulnerables a perturbaciones naturales y antropogénicas, dentro de las cuales la fragmentación representa una mayor amenaza. Consecuente con lo anterior, han sido identificados como valor objeto de conservación del PNN Los Corales del Rosario y de San Bernardo (PNNCRSB). En particular, las especies más representativas son el mangle rojo Rhizophora mangle, el zaragoza Conocarpus erectus y el amarillo Laguncularia racemosa. Actualmente, la información existente y el programa de monitoreo de esta área protegida se limita a datos de coberturas, por lo que no existe información disponible sobre la fenología de estas especies. Por lo tanto, esta investigación tuvo como objetivo evaluar la variación de eventos fenológicos (producción de frutos, flores y hojas) de mangles rojo, zaragoza y amarillo en el PNNCRSB durante 2014-2015 y su relación con patrones climáticos interanuales. Los resultados muestran que existen patrones temporales de la variación de los eventos fenológicos. En el caso de R. mangle, no evidenció caída de hojas, pero sí presentó frutos y flores durante los 24 meses. Algo similar se observó para C. erectus. También se registraron correlaciones canónicas robustas y significativas (R>6; p<0,05) de grupos de variables ambientales climáticas y los eventos fenológicos en las tres especies en los dos años de estudio. De esta forma se infiere que existen condiciones ambientales idóneas para el desarrollo de los mangles y que los patrones climáticos inciden sobre la fenología de estas especies en el área de estudio. Finalmente, las características fenológicas de las especies se proponen como insumo importante para la adopción de medidas de manejo como procesos de restauración, pues permiten conocer los periodos indicados para realizar actividades de recolecta de propágulos, montaje de viveros y siembra de plántulas en zonas degradadas.
A dívida pública como instrumento de extermínio no Estado de Exceção
Authors: de Oliveira Diniz Carlos Augusto
Published: July 2023

O objeto do presente trabalho é o conceito de estado de exceção em suas múltiplas faces. A hipótese repousa na ideia de que no Brasil a dívida pública funciona como instrumento de suspensão dos direitos fundamentais. O objetivo geral é analisar a dívida pública brasileira e o objetivo específico é confrontar a dívida pública com a falta de efetividade dos direitos sociais em decorrência de políticas ditas de austeridade fiscal. Optamos pelo método dedutivo partindo de premissa geral que é a análise da dívida pública para o aspecto específico da efetividade dos direitos sociais. A conclusão do trabalho demonstra que o conceito de estado de exceção transcende o campo do direito penal e processo penal e também se evidencia em outros ramos do direito como o financeiro e orçamentário.