bluuebunny/crossref_metadata_2025 huggingface dataset:
Encapsulation of miRNA and siRNA into Nanomaterials for Cancer Therapeutics
Authors: Zare Mina , Pemmada Rakesh , Madhavan Maya , Shailaja Aswathy , Ramakrishna Seeram , Kandiyil Sumodan Padikkala , Donahue James M. , Thomas Vinoy
Published: August 2022

Globally, cancer is amongst the most deadly diseases due to the low efficiency of the conventional and obsolete chemotherapeutic methodologies and their many downsides. The poor aqueous solubility of most anticancer medications and their low biocompatibility make them ineligible candidates for the design of delivery systems. A significant drawback associated with chemotherapy is that there are no advanced solutions to multidrug resistance, which poses a major obstacle in cancer management. Since RNA interference (RNAi) can repress the expression of genes, it is viewed as a novel tool for advanced drug delivery. this is being explored as a promising drug targeting strategy for the treatment of multiple diseases, including cancer. However, there are many obstructions that hinder the clinical uses of siRNA drugs due to their low permeation into cells, off-target impacts, and possible unwanted immune responses under physiological circumstances. Thus, in this article, we review the design measures for siRNA conveyance frameworks and potential siRNA and miRNA drug delivery systems for malignant growth treatment, including the use of liposomes, dendrimers, and micelle-based nanovectors and functional polymer–drug delivery systems. This article sums up the advancements and challenges in the use of nanocarriers for siRNA delivery and remarkably centers around the most critical modification strategies for nanocarriers to build multifunctional siRNA and miRNA delivery vectors. In short, we hope this review will throw light on the dark areas of RNA interference, which will further open novel research arenas in the development of RNAi drugs for cancer.
Orally Dispersible Dosage Forms for Paediatric Use: Current Knowledge and Development of Nanostructure-Based Formulations
Authors: Cornilă Andreea , Iurian Sonia , Tomuță Ioan , Porfire Alina
Published: August 2022

The paediatric population has always suffered from a lack of medicines tailored to their needs, especially in terms of accurate dosage, stability and acceptability. Orodispersible dosage forms have gone through a resurrection as an alternative to liquid formulations or fractioned solid formulations, although they are still subject to several inconveniences, among which the unpleasant taste and the low oral bioavailability of the API are the most significant hurdles in the way of achieving an optimal drug product. Nanostructures can address these inconveniences through their size and variety, owing to the plethora of materials that can be used in their manufacturing. Through the formation and functionalisation of nanostructures, followed by their inclusion in orodispersible dosage forms, safe, stable and acceptable medicines intended for paediatric use can be developed.
Modifying the Stability and Surface Characteristic of Anthocyanin Compounds Incorporated in the Nanoliposome by Chitosan Biopolymer
Authors: Homayoonfal Mina , Mousavi Mohammad , Kiani Hossein , Askari Gholamreza , Desobry Stephane , Arab-Tehrany Elmira
Published: August 2022

In this study, a novel approach was investigated to improve the stability of anthocyanin compounds (AC) by encapsulating them in nanoliposomes resulting from rapeseed lecithin alongside chitosan coating. The results indicate that the particle size, electrophoretic mobility, encapsulation efficiency, and membrane fluidity of nanoliposomes containing anthocyanin compounds were 132.41 nm, −3.26 µm·cm/V·S, 42.57%, and 3.41, respectively, which changed into 188.95 nm, +4.80 µm·cm/V·S, 61.15%, and 2.39 after coating with chitosan, respectively. The results also suggest improved physical and chemical stability of nanoliposomes after coating with chitosan. TEM images demonstrate the produced particles were spherical and had a nanoscale, where the existence of a chitosan layer around the nanoparticles was visible. Shear rheological tests illustrate that the flow behavior of nanoliposomes was altered from Newtonian to shear thinning following chitosan incorporation. Further, chitosan diminished the surface area of the hysteresis loop (thixotropic behavior). The oscillatory rheological tests also show the presence of chitosan led to the improved mechanical stability of nanoliposomes. The results of the present study demonstrate that chitosan coating remarkably improved encapsulation efficiency, as well as the physical and mechanical stability of nanoliposomes. Thus, coating AC-nanoliposomes with chitosan is a promising approach for effective loading of AC and enhancing their stability to apply in the pharmaceutic and food industries.
Supercritical Antisolvent Technique for the Production of Breathable Naringin Powder
Authors: Adami Renata , Russo Paola , Amante Chiara , De Soricellis Chiara , Della Porta Giovanna , Reverchon Ernesto , Del Gaudio Pasquale
Published: August 2022

Flavonoids are polyphenolic compounds largely present in fruits and vegetables possessing antioxidant properties, anti-inflammatory and antibacterial activities. Their use in clinical practice is very poor due to their low bioavailability, susceptibility to oxidation and degradation. Moreover, their slight solubility in biological fluids and a consequent low dissolution rate leads to an irregular absorption from solid dosage forms, even though, anti-inflammatory formulations could be used as support for several disease treatment, i.e. the COVID-19 syndrome. To improve flavonoid bioavailability particle size of the powder can be reduced to make it breathable and to promote the absorption in the lung tissues. Supercritical fluid based antisolvent technique has been used to produce naringin particles, with size, shape and density as well as free flowing properties able to fit inhalation needs. The dried particles are produced with the removal of the solvent at lower temperatures compared to the most used traditional micronization processes, such as spray drying. The best breathable fraction for naringin particles is obtained for particles with a d50~7 µm manufactured at 35 °C-150 bar and at 60 °C-130 bar, corresponding to 32.6% and 36.7% respectively. The powder is produced using a high CO2 molar fraction (0.99) that assure a better removal of the solvent. NuLi-1 cell line of immortalised bronchial epithelial cells adopted to evaluate powder cytotoxicity indicated after 24 h absence of toxicity at concentration of 25 µM.
Potential Combinatory Effect of Cannabidiol and Triclosan Incorporated into Sustained Release Delivery System against Oral Candidiasis
Authors: Feldman Mark , Gati Irith , Sionov Ronit Vogt , Sahar-Helft Sharonit , Friedman Michael , Steinberg Doron
Published: August 2022

Candida albicans is a common fungal pathogen. Biofilm formation on various surfaces is an important determinant of C. albicans pathogenicity. Our previous results demonstrated the high potential of cannabidiol (CBD) to affect C. albicans biofilms. Based on these data, we investigated the possibility of incorporating CBD and/or triclosan (an antimicrobial agent that is widely utilized in dentistry) in a sustained-release varnish (SRV) (SRV-CBD, SRV-triclosan) to increase their pharmaceutical potential against C. albicans biofilm, as well as that of the mixture of the agents into SRV (SRV-CBD/triclosan). The study was conducted in a plastic model, on agar, and in an ex vivo tooth model. Our results demonstrated strong antibiofilm activity of SRV-CBD and SRV-triclosan against C. albicans in all tested models. Both formulations were able to inhibit biofilm formation and to remove mature fungal biofilm. In addition, SRV-CBD and SRV-triclosan altered C. albicans morphology. Finally, we observed a dramatic enhancement of antibiofilm activity when combined SRV-CBD/triclosan was applied. In conclusion, we propose that incorporation of CBD or triclosan into SRV is an effective strategy to fight fungal biofilms. Importantly, the data demonstrate that our CBD/triclosan varnish is safe, and is not cytotoxic for normal mammalian cells. Furthermore, we propose that CBD and triclosan being in mixture in SRV exhibit complementary antibiofilm activity, and thus can be explored for further development as a potential treatment against fungal infections.
The Finite Element Analysis Research on Microneedle Design Strategy and Transdermal Drug Delivery System
Authors: Yan Qinying , Shen Shulin , Wang Yan , Weng Jiaqi , Wan Aiqun , Yang Gensheng , Feng Lili
Published: August 2022

Microneedles (MNs) as a novel transdermal drug delivery system have shown great potential for therapeutic and disease diagnosis applications by continually providing minimally invasive, portable, cost-effective, high bioavailability, and easy-to-use tools compared to traditional parenteral administrations. However, microneedle transdermal drug delivery is still in its infancy. Many research studies need further in-depth exploration, such as safety, structural characteristics, and drug loading performance evaluation. Finite element analysis (FEA) uses mathematical approximations to simulate real physical systems (geometry and load conditions). It can simplify complex engineering problems to guide the precise preparation and potential industrialization of microneedles, which has attracted extensive attention. This article introduces FEA research for microneedle transdermal drug delivery systems, focusing on microneedle design strategy, skin mechanics models, skin permeability, and the FEA research on drug delivery by MNs.
Design, Synthesis, and Characterization of Novel Coordination Compounds of Benzimidazole Derivatives with Cadmium
Authors: Raducka Anita , Świątkowski Marcin , Korona-Głowniak Izabela , Kaproń Barbara , Plech Tomasz , Szczesio Małgorzata , Gobis Katarzyna , Czylkowska Agnieszka
Published: August 2022

Four complexes of Cd(II) with benzimidazole derivatives were synthesized and named C1, C2, C3, and C4. All coordination compounds were characterized through elemental analysis (EA), flame atomic absorption spectrometry (FAAS), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis coupled with mass spectrometry) (TG-MS), a cytotoxicity assay (MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide)), and computational chemical analysis for absorption, distribution, metabolism, and excretion (ADME). All of the obtained results are compatible and are consistent with the respective structures of the obtained compounds and their properties. The various techniques used allowed the determination of the composition, proposed structure of the compounds, their thermal stability and thermal properties, and the method of coordination between the metal (II) ion and the ligand. The ADME technique was also used to estimate the physicochemical and biological properties. The antitumor activity of the compounds was determined with an MTT assay on the glioblastoma (T98G), neuroblastoma (SK-N-AS), and lung adenocarcinoma (A549) cell lines, as well as normal human skin fibroblasts (CCD-1059Sk). Compound C2 was found to have potential antitumor properties and to be effective in inhibiting the growth of neuroblastoma cells. The antimicrobial activity of Cd complexes, free ligands, and reference drugs was tested against six strains of Gram-positive bacteria, five strains of Gram-negative rods, and three strains of yeasts. Compound C3 significantly increased activity against Gram-positive bacteria in comparison to the ligand.
High Molecular Weight Hyaluronic Acid Reduces the Expression of Virulence Genes fimA, mfa1, hagA, rgpA, and kgp in the Oral Pathogen Porphyromonas gingivalis
Authors: Alharbi Meshal S. , Alshehri Fahad A.
Published: August 2022

Porphyromonas gingivalis (P. gingivalis) is a cornerstone pathogen in the development and progression of periodontal and peri-implant tissue destruction. It is capable of causing dysbiosis of the microbial biofilm and modulation of the host immune system. Hyaluronic acid (HA) is a naturally occurring glycosaminoglycan found in all living organisms. It is well known and has been used for improving tissue healing. In addition, some studies have suggested that there may be an antimicrobial potential to HA. The aim of this study was to evaluate the effect of hyaluronic acid, azithromycin (AZM), and chlorhexidine (CHX) on the expression of genes (i.e., fimA, mfa1, hagA, rgpA, rgpB, and kgp) related to the virulence and adhesion of P. gingivalis. The study groups were divided into four: (1) HA treated group; (2) AZM treated group; (3) CHX treated group; and (4) untreated group to serve as a negative control. P. gingivalis ATCC 33277 was cultured and then exposed to four different concentrations (100% MIC, 50% MIC, 25% MIC, and 12.5% MIC) of HA, AZM, and CHX for 24 h. The expression levels of the aforementioned genes were measured using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Relative fold-change values were calculated and compared between groups. The fold-change values of all genes combined were 0.46 ± 0.33, 0.31 ± 0.24, and 0.84 ± 0.77 for HA, AZM, and CHX, respectively. HA has downregulated all the genes by mostly a half-fold: 0.35 ± 0.20, 0.47 ± 0.35, 0.44 ± 0.25, 0.67 ± 0.46, 0.48 ± 0.33 and 0.35 ± 0.22 with fimA, mfa1, hagA, rgpA, rgpB and kgp, respectively. The effect of HA was significant on all genes except rgpB compared to the untreated control. Lower concentrations of HA tended to exhibit greater downregulation with 1 mg/mL being the most effective. High molecular weight (1.5 MDa) hyaluronic acid has a potent effect on P. gingivalis by downregulating fimA, mfa1, hagA, rgpA, and kgp. The effect of HA was generally less than that of AZM but greater than that of CHX.
All-Fiber Photoacoustic Gas Sensing with Interferometric Location
Authors: Li Meng , Hu Mengpeng , Zhang Hui , Wang Jianing , Tang Tongyu , Hu Mai , Wang Qiang
Published: August 2022

Photoacoustic spectroscopy (PAS) is a promising gas detection technique with high sensitivity, fast response, and good stability. Frequency-modulated continuous-wave (FMCW) interferometry offers precise distance detection with high spatial resolution. The combination of PAS and FMCW may lead to an optical technique for the simultaneous extraction of gas concentration and location information. Herein, we demonstrate this technique in an all-fiber sensing system by blending a fiber-pigtailed PAS sensor with an FMCW interferometer. As an example, we have measured the methane concentration and location by employing time-division multiplexing, showing a minimum detection limit of 28 ppm and a spatial resolution of 3.87 mm over a distance of ~4.9 m. This study enables the realization of a versatile technique for multiparameter gas sensing in gas leakage detection and gas emission monitoring.
Effects of Melt Temperature and Non-Isothermal Flow in Design of Coat Hanger Dies Based on Flow Network of Non-Newtonian Fluids
Authors: Razeghiyadaki Amin , Wei Dongming , Perveen Asma , Zhang Dichuan , Wang Yanwei
Published: August 2022

In the design of coat hanger extrusion dies, the main objective is to provide a uniform flow rate at the die exit. Previously, a multi-rheology isothermal method model for coat hanger extrusion dies was developed to reach this objective. Polymer melts in extrusion dies commonly experience high shear rates. Viscous dissipation rooted by high shear rate may lead to significant temperature differences across the die. Due to temperature-dependency of viscosity, temperature differences may lead to nonuniform flow rates, which may significantly affect the flow rate at the die exit. As a result, a new design method is proposed to take into account the effects of temperature and viscous dissipation in the design of coat hanger dies. Although more non-Newtonian fluid rheology models can be adapted in the proposed study, as demonstration, temperature-dependent power-law and Carreau–Yasuda models are adapted in this study. Performances are compared with our isothermal method published earlier. In addition, the novel nonisothermal method is comprehensively examined where the effect of viscous dissipation is studied through Brinkman number of extrusion die. It is demonstrated that, for a low Brinkman number, both isothermal and nonisothermal design give similar flow uniformity level. However, for higher Brinkman numbers, the proposed nonisothermal method produces a design with more desirable velocity uniformity level along with a maximum improvement of 5.24% over the isothermal method. In addition, dependency of flow field on temperature, due to temperature-dependent viscosity, is studied, and it is demonstrated that fully-developed velocity profile changes as temperature increases along the flow channel. Moreover, the effect of the temperature sensitivity parameter in temperature-dependent non-Newtonian models is considered. It is demonstrated that the temperature boundary condition with the Biot number of 1.0 gives adequate results for lower values of the temperature sensitivity parameter.
A Promising Review on Cyclodextrin Conjugated Paclitaxel Nanoparticles for Cancer Treatment
Authors: Velhal Kamini , Barage Sagar , Roy Arpita , Lakkakula Jaya , Yamgar Ramesh , Alqahtani Mohammed S. , Yadav Krishna Kumar , Ahn Yongtae , Jeon Byong-Hun
Published: August 2022

This review presented the unique characteristics of different types of cyclodextrin polymers by non-covalent host–guest interactions to synthesize an inclusion complex. Various cancers are treated with different types of modified cyclodextrins, along with the anticancer drug paclitaxel. PTX acts as a mitotic inhibitor, but due to its low dissolution and permeability in aqueous solutions, it causes considerable challenges for drug delivery system (DDS) designs. To enhance the solubility, it is reformulated with derivatives of cyclodextrins using freeze-drying and co-solvent lyophilization methods. The present supramolecular assemblies involve cyclodextrin as a key mediator, which is encapsulated with paclitaxel and their controlled release at the targeted area is highlighted using different DDS. In addition, the application of cyclodextrins in cancer treatment, which reduces the off-target effects, is briefly demonstrated using various types of cancer cell lines. A new nano-formulation of PTX is used to improve the antitumor activity compared to normal PTX DDS in lungs and breast cancer is well defined in the present review.
Magnetic Graphene-Based Nanosheets with Pluronic F127-Chitosan Biopolymers Encapsulated α-Mangosteen Drugs for Breast Cancer Cells Therapy
Authors: Hardiansyah Andri , Randy Ahmad , Dewi Rizna Triana , Angelina Marissa , Yudasari Nurfina , Rahayu Sri , Ulfah Ika Maria , Maryani Faiza , Cheng Yu-Wei , Liu Ting-Yu
Published: August 2022

In this study, multifunctional chitosan-pluronic F127 with magnetic reduced graphene oxide (MRGO) nanocomposites were developed through the immobilization of chitosan and an amphiphilic polymer (pluronic F127) onto the MRGO. Physicochemical characterizations and in-vitro cytotoxicity of nanocomposites were investigated through field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, particle size analysis, vibrating sample magnetometer, Raman spectroscopy and resazurin-based in-vitro cytotoxicity assay. FESEM observation shows that the magnetic nanoparticles could tethered on the surface of MRGO, promoting the magnetic properties of the nanocomposites. FTIR identification analysis revealed that the chitosan/pluronic F127 were successfully immobilized on the surface of MRGO. Furthermore, α-mangosteen, as a model of natural drug compound, was successfully encapsulated onto the chitosan/pluronic F127@MRGO nanocomposites. According to in-vitro cytotoxicity assay, α-mangosteen-loaded chitosan/pluronic F127@MRGO nanocomposites could significantly reduce the proliferation of human breast cancer (MFC-7) cells. Eventually, it would be anticipated that the novel α-mangosteen-loaded chitosan/pluronic F127@MRGO nanocomposites could be promoted as a new potential material for magnetically targeting and killing cancer cells.
Tuning Thermal, Morphological, and Physicochemical Properties of Thermoplastic Polyurethanes (TPUs) by the 1,4-Butanediol (BDO)/Dipropylene Glycol (DPG) Ratio
Authors: Ernzen Juliano R. , Romoaldo Carlos H. , Gommes Cedric , Covas José A. , Marcos-Fernández Angel , Fiorio Rudinei , Bianchi Otávio
Published: August 2022

Thermoplastic polyurethanes (TPUs) are versatile polymers presenting a broad range of properties as a result of their countless combination of raw materials—in essence, isocyanates, polyols, and chain extenders. This study highlights the effect of two different chain extenders and their combination on the structure–property relationships of TPUs synthesized by reactive extrusion. The TPUs were obtained from 4,4-diphenylmethane diisocyanate (MDI), polyester diols, and the chain extenders 1,4-butanediol (BDO) and dipropylene glycol (DPG). The BDO/DPG ratios studied were 100/0, 75/25, 50/50, 25/75, and 0/100 wt.%. The TPUs were characterized by size exclusion chromatography (SEC), Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), small-angle X-ray scattering (SAXS), UV–vis spectroscopy, and physical-mechanical properties. The results indicate that DPG promotes compatibility between rigid (HS) and flexible (SS) segments of TPUs. Consequently, increasing DPG content (>75 wt.%) reduced the organization of the rigid segments and the degree of phase separation, increasing the polydispersity of the interdomain distance and the transparency in the UV–visible spectrum of the TPUs. Furthermore, increasing DPG content also reduced the amount of hydrogen bonds present in the rigid phase, reducing or extinguishing its glass transition temperature (TgHS) and melting temperature (Tm), and increasing the glass transition temperature of the flexible phase (TgSS). Therefore, increasing DPG content leads to a deterioration in mechanical properties and hydrolysis resistance.
Controlled Cationic Polymerization of p-Methylstyrene in Ionic Liquid and Its Mechanism
Authors: Zhang Xiaoqian , Tang Shengde , Gao Ming , Sun Chunfeng , Wang Jiasheng
Published: August 2022

Ionic liquid (IL) as a green solvent is entirely composed of ions; thus, it may be more than a simple solvent for ionic polymerization. Here, the cationic polymerization of p-methylstyrene (p-MeSt) initiated by 1-chloro-1-(4-methylphenyl)-ethane (p-MeStCl)/tin tetrachloride (SnCl4) was systematically studied in 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([Bmim][NTf2]) IL at −25 °C. The results show that IL did not participate in cationic polymerization, but its ionic environment and high polarity were favorable for the polarization of initiator and monomer and facilitate the controllability. The gel permeation chromatography (GPC) trace of the poly(p-methylstyrene) (poly(p-MeSt)) changes from bimodal in dichloromethane (CH2Cl2) to unimodal in IL, and polydispersities Mw/Mn of the polymer in IL showed narrower (1.40–1.59). The reaction rate and heat release rate were milder in IL. The effects of the initiating system, Lewis acid concentration, and 2,6-di-tert-butylpyridine (DTBP) concentration on the polymerization were investigated. The controlled cationic polymerization initiated by p-MeStCl/SnCl4 was obtained. The polymerization mechanism of p-MeSt in [Bmim][NTf2] was also proposed.
Adsorption of Hydrophobic Ions on Environmentally Relevant Sorbents
Authors: Becskereki Gergely , Horvai George , Tóth Blanka
Published: August 2022

Environmental monitoring and remediation often requires the collection of harmful substances from aqueous solutions. Absorption with solids is a useful technique for binding such substances even at very low concentration levels. Many of these contaminants are weak acids or bases. Some novel, nonionic polymeric sorbents, such as hypercrosslinked polymers or polymers with balanced hydrophilic-lipophilic properties (HLB) have been found to bind weak acids and bases with high distribution coefficients even at pH values where these compounds are almost completely ionized (typically near pH 7). To understand this phenomenon and its practical consequences, we have experimentally studied the adsorption of ionizable weak acids and bases as a function of pH and ionic strength on a the OASIS® HLB sorbent. Not surprisingly, the ionic forms of the weak acids and bases were found to be much less bound in the aqueous solution than their neutral forms. In spite of this, OASIS® HLB binds weak acids and bases around pH 7 considerably better than typical hydrophobic sorbents. The high overall distribution coefficients around pH 7 could be explained by two factors. One is that on OASIS® HLB, and on some other novel polymeric sorbents, the binding constant of the moderately hydrophobic neutral form is on the order of 100,000, i.e., much higher than on typical hydrophobic sorbents. Thus, even if the proportion of the neutral form in solution is only around 1% near pH 7, the adsorption of the neutral form is still significant. On the other hand, the binding of the apparently hydrophilic ionized forms occurs with distribution coefficients well above 100. The distribution coefficient of the ionic form appears to depend on ionic strength and the presence of competing ions. Adsorption of the ionic forms is found to be very similar to the adsorption of ionic surfactants. The pH dependence of the total adsorption of neutral and ionic forms together, is found to be steep around pH 7, and therefore the varying pH of natural waters may strongly influence the binding efficiency in practical applications, such as the collection (concentration) of contaminants or their passive sampling.
Thiolated 2-Methyl-β-Cyclodextrin as a Mucoadhesive Excipient for Poorly Soluble Drugs: Synthesis and Characterization
Authors: Grassiri Brunella , Cesari Andrea , Balzano Federica , Migone Chiara , Kali Gergely , Bernkop-Schnürch Andreas , Uccello-Barretta Gloria , Zambito Ylenia , Piras Anna Maria
Published: August 2022

Thiolated cyclodextrins are structurally simple mucoadhesive macromolecules, which are able to host drugs and increase their apparent water solubility, as well as interact with the mucus layer prolonging drug residence time on the site of absorption. The aim of this study was to synthesize through green microwave-assisted process a freely soluble thiolated 2-methyl-β-cyclodextrin (MβCD-SH). Its inclusion complex properties with dexamethasone (Dex), a poor water soluble drug, and mucoadhesive characteristics were also determined. The product was deeply characterized through NMR spectroscopy (2D COSY, 2D HSQC, 1D/2D TOCSY, and 1D ROESY), showing a thiolation degree of 67%, a selective thiolation on the C6 residues and a monomeric structure. The association constant of MβCD and MβCD-SH with Dex resulted in 2514.3 ± 32.3 M−1 and 2147.0 ± 69.3 M−1, respectively, indicating that both CDs were able to host the drug. Microrheological analysis of mucin in the presence of MBCD-SH showed an increase of complex viscosity, G′ and G″, due to disulphide bond formation. The cytotoxicity screening on fibroblast BALB/3T3 clone A31 cells indicated an IC50 of 27.7 mg/mL and 30.0 mg/mL, for MβCD and MβCD-SH, respectively. Finally, MβCD-SH was able to self-assemble in water into nanometric structures, both in the presence and absence of the complexed drug.
Thermo-Responsive Polyion Complex of Polysulfobetaine and a Cationic Surfactant in Water
Authors: Pham Thu Thao , Yusa Shin-ichi
Published: August 2022

Poly(4-((3-methacrylamidopropyl)dimethylammonium)butane-1-sulfonate) (PSBP) was prepared via controlled radical polymerization. PSBP showed upper critical solution temperature (UCST) behavior in aqueous solutions, which could be controlled by adjusting the polymer and NaCl concentrations. Owing to its pendant sulfonate anions, PSBP exhibited a negative zeta potential of −7.99 mV and formed a water-soluble ion complex with the cationic surfactant cetyltrimethylammonium bromide (CTAB) via attractive electrostatic interaction. A neutral PSBP/CTAB complex was formed under equimolar concentrations of the pendant sulfonate group in PSBP and the quaternary ammonium group in CTAB. Transmittance electron microscopic images revealed the spherical shape of the complex. The stoichiometrically neutral-charge PSBP/CTAB complex exhibited UCST behavior in aqueous solutions. Similar to PSBP, the phase transition temperature of the PSBP/CTAB complex could be tuned by modifying the polymer and NaCl concentrations. In 0.1 M aqueous solution, the PSBP/CTAB complex showed UCST behavior at a low complex concentration of 0.084 g/L, whereas PSBP did not exhibit UCST behavior at concentrations below 1.0 g/L. This observation suggests that the interaction between PSBP and CTAB in the complex was stronger than the interpolymer interaction of PSBP.
Tyramine-Functionalized Alginate-Collagen Hybrid Hydrogel Inks for 3D-Bioprinting
Authors: Kim Sung Dong , Jin Subin , Kim Sumin , Son Donghee , Shin Mikyung
Published: August 2022

Extrusion-based 3D-bioprinting using hydrogels has exhibited potential in precision medicine; however, researchers are beset with several challenges. A major challenge of this technique is the production of constructs with sufficient height and fidelity to support cellular behavior in vivo. In this study, we present the 3D-bioprinting of cylindrical constructs with tunable gelation kinetics by controlling the covalent crosslinking density and gelation time of a tyramine-functionalized alginate hydrogel (ALG-TYR) via enzymatic reaction by horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The extruded filament was crosslinked for a second time on a support bath containing H2O2 to increase fidelity after printing. The resulting tubular construct, with a height of 6 mm and a wall thickness of 2 mm, retained its mechanical properties and had a maximum 2-fold swelling after 2 d. Furthermore, collagen (COL) was introduced into the ALG-TYR hydrogel network to increase the mechanical modulus and cell cytocompatibility, as the encapsulated fibroblast cells exhibited a higher cell viability in the ALG-TYR/COL construct (92.13 ± 0.70%) than in ALG-TYR alone (68.18 ± 3.73%). In summary, a vascular ECM-mimicking scaffold was 3D-bioprinted with the ALG-TYR/COL hybrid hydrogel, and this scaffold can support tissue growth for clinical translation in regenerative and personalized medicine.
Some Mechanical Properties of Composite Materials with Chopped Wheat Straw Reinforcer and Hybrid Matrix
Authors: Bolcu Dumitru , Stănescu Marius Marinel , Miriţoiu Cosmin Mihai
Published: August 2022

Modern agriculture produces a very large amount of agricultural waste that remains unused. The use as a reinforcer of these renewable resources for the realization of composite materials, and the finding of useful industrial applications, constitutes or provokes the groups of researchers in this field. The study conducted in this article falls in this direction. Composites were fabricated with the chopped wheat straw reinforcement and epoxy resin matrix or hybrid resins with 50% and 70% Dammar volume proportions. Some mechanical properties of this type of composite materials were studied based on tensile strength, SEM analysis, water absorption/loss, vibration behavior and compression strength. The strength–strain and strain–strain diagrams, the modulus of elasticity, the breaking strength and the elongation at break were obtained. Compared to the epoxy resin composition, those with 50 and 70% Dammar, respectively, have a 47 and 55% lower breaking strength and a 30 and 84% higher damping factor, respectively. Because the values of these mechanical properties were limited, and in practice superior properties are needed, sandwich composites were manufactured, with the core of previously studied compositions, to which the outer faces of linen fabric were applied. These composites were applied to the bend (in three points), obtaining the force–deformation diagrams. The obtained properties show that they can be used in construction (paneling, shells, etc.), or in the furniture industry.
Performance-Matching Optimization Design of Loader-Hydraulic System Based on Hydrodynamics Analysis
Authors: Wang Haifei , Yang Shimin , Lu Tan
Published: August 2022

The study of the performance of dynamic hydraulic throttling under the condition of stable fluid is of great significance. The effect of a step change in pressure differences on the throttling performance of a hydraulic valve is studied. This paper studies the dynamic and static performance of a hydraulic-valve-outlet throttling-speed regulation system, builds a more accurate mathematical model, considers the linear factors of the flow of hydraulic-valve throttling, analyzes the influence of the step-load change in pressure difference on the stability of the hydraulic-valve movement speed, and constructs a nonlinear mathematical model of the speed-regulation system of the outlet throttling. A pressure sensor is used to measure the change in pressure overshoot, and the effect of a pressure-difference step change on the throttling performance of the hydraulic valve is studied under steady-fluid conditions. The theory is analyzed and verified by experiment, and the parameters of hydraulic components are modified using the dynamic-change rule of the hydraulic valve’s two-chamber pressure.
The Making of a Sacred Landscape: Visualizing Hangzhou Buddhist Culture via Geoparsing a Local Gazetteer the Xianchun Lin’an zhi 咸淳臨安志
Authors: Liu Jeffrey , Wan Ziling
Published: August 2022

This project uses local sources to visualize and analyze the spatial distribution of Buddhist sites in Hangzhou 杭州, China, in the Southern Song dynasty (1127–1279). It aims to highlight regional religious features in Hangzhou as a locality—the interactions between Buddhism and sociocultural factors—from the visualization and analyses. With the advent of the spatial turn in the field of humanities, numerous endeavors have been undertaken to collect data from religious sites in East Asia. However, the collections are aimed at a nationwide-level scale rather than targeted at regional aspects. Studying religion by using the data of large-scale areas often prevents us from observing regional characteristics such as how religion interacted with local factors. Hence, this project draws spatial data from a Hangzhou local gazetteer titled the Xianchun Lin’an zhi 咸淳臨安志 (Records about Lin’an from the Xianchun Reign, a 100-fascicle local chronicle that depicted the Lin’an Prefecture in the Southern Song dynasty) to create a visualization for all Buddhist establishments in Hangzhou. We observe how a religious landscape within a locality is portrayed when it was renowned as a political, cultural, and economic center at a given time. Starting as a project led by him in 2020, Jiang Wu’s team converted all Buddhist temple locations recorded in the Xianchun Lin’an zhi into geographical coordinates. Based on the dataset, we analyze the distribution of Buddhist temples with the application of GIS via three methods: average nearest neighbor, quadrat analysis, and kernel density to highlight localism and regionalism in Chinese religious studies. Our results of GIS distant reading indicate a highly clustered congregation of Buddhist temples in Hangzhou. Corroborating the results of distant reading with factual information (recorded in historical materials) from close reading, we discover that the spatial pattern of Buddhist temples is correlated with socio-political factors including fengshui, state power, politics, and commercial exchanges. With the combination of distant reading and close reading, we can highlight the interactions between Buddhism and socio-political factors that are not easily spotted via traditional textual approaches or using data that is scaled nationwide.
Charismatic Pneumatology as Ecumenical Opportunity: Orthopraxy, Subjectivity, and Relational Ontologies of the Holy Spirit
Authors: Alvis Jason Wesley
Published: August 2022

The majority of new Christian communities have been appearing in Charismatic and Pentecostal movements, especially in the Global South. Along with these shifts emerge new possibilities to better understand the diversity of Christian perspectives and to rethink what it means to be “in relation” to a global Christian community. After opening connections between pneumatology and relational ontology, this article engages the work of three emerging Pentecostal/Charismatic thinkers in particular, whose pneumatologies provide novel opportunities to think more carefully about “relationality” and ecumenical unity: Nimi Wariboko, Amos Yong, and Clark Pinnock. Wariboko’s pneumatology helps us acknowledge the very kind of relational ontology God has with Godself, as a split subject, thereby disrupting not only our all-too-human meaning-making process, but also the way God signifies the world for us. Yong’s pneumatology emphasizes human practice or an “orthopraxy” that is polyphonous, historically rooted, and oriented around spiritual gifts not only for sanctification but also for worldwide witness. Finally, Pinnock emphasizes the connections between creativity and relationality, pointing to how at-one-ment is also the telic work of the Spirit.
Investor Segments by Perceived Project Risk and Their Characteristics Based on Primary Research Results
Authors: Garai-Fodor Mónika , Szemere Tibor Pál , Csiszárik-Kocsir Ágnes
Published: August 2022

Our research focuses on investment initiatives. The perceived risks are analysed from the consumer’s viewpoint, i.e., the client’s perspective, from the standpoint of the project. In the literature, there are several risk interpretations and classifications. We assumed it could be interesting and valuable to approach investment projects from a consumer-oriented viewpoint, considering that the perceived and consumer-identified set of risks is a major determinant of the outcome of a decision. In addition to relevant secondary sources, we also present partial results of our primary research project. A pre-tested, standardised online questionnaire was employed in the primary study, using a snowball sampling approach generating 1545 evaluable questionnaires. As a result of the research, we were able to segment the customer (investor) target groups into various categories depending on the perceived project risk. We have established the orientation directions along which these segments may well be meaningfully described in terms of perceived investment risk concerns and socio-demographic characteristics that influence cluster membership. In our opinion, the findings may be a useful source of information for investment project developers looking to identify consumer groups based on risk perception and build project solutions for them.
Evaluation of Drought Vulnerability of Maize and Influencing Factors in Songliao Plain Based on the SE-DEA-Tobit Model
Authors: Ma Yining , Guga Suri , Xu Jie , Liu Xingpeng , Tong Zhijun , Zhang Jiquan
Published: August 2022

Rain-fed agriculture is easily affected by meteorological disasters, especially drought. As an important factor of risk formation, actively carrying out agricultural drought vulnerability assessments is conducive to improving food security and reducing economic losses. In this study, an SE-DEA model with regional exposure and drought risk as input factors and the maize yield reduction rate and drought-affected area as output factors is established. The aim is to evaluate and zone the drought vulnerability of the maize belt in the Songliao Plain. The results show the following: (1) From 2000 to 2019, the drought vulnerability of maize showed a fluctuating increasing trend. The vulnerability in Harbin and central Jilin Province is high, which is extremely unfavorable for maize production. (2) Comparing the historical disaster data with the drought vulnerability map generated using the SE-DEA model, it could be found that the results obtained using the SE-DEA model are reliable. (3) The Tobit model shows that the proportion of the effective irrigated area is more important to alleviate vulnerability. For drought vulnerability zoning using a cluster analysis, we suggest that regulated deficit irrigation should be actively developed in high-vulnerability areas to ensure maize yield while improving water efficiency. The results of this study can provide a basis for the development of drought mitigation and loss reduction strategies, and they provide new ideas for future research.
Analysis of Maize Sowing Periods and Cycle Phases Using Sentinel 1&2 Data Synergy
Authors: Rolle Matteo , Tamea Stefania , Claps Pierluigi , Ayari Emna , Baghdadi Nicolas , Zribi Mehrez
Published: August 2022

The reliability of crop-growth modelling is related to the accuracy of the information used to describe the agricultural growing phases. A proper knowledge of sowing periods has a significant impact on the effectiveness of any analysis based on modeled crop growth. In this work, an estimation of maize actual sowing periods for year 2019 is presented, combining the optical and radar information from Sentinel-1 and Sentinel-2. The crop classification was conducted according to the information provided by local public authorities over an area of 30 km × 30 km, and 1154 maize fields were considered within the analysis. The combined use of NDVI and radar time series enabled a high-resolution assessment of sowing periods and the description of maize emergence through the soil, by detecting changes in the ground surface geometry. A radar-based index was introduced to detect the periods when plants emerge through the soil, and the sowing periods were retrieved considering the thermal energy needed by seeds to germinate and the daily temperatures before the emergence. Results show that 52% of maize hectares were sowed in late April, while about 30% were sowed during the second half of May. Sentinel-1 appears more suitable to describe the late growing phase of maize, since the radar backscattering is sensitive to the dry biomass of plants while the NDVI decreases because of the chromatic change of leaves. This study highlights the potential of synergy between remote sensing sources for agricultural management policies and improving the accuracy of crop-related modelling.
Marine Oil Spill Detection with X-Band Shipborne Radar Using GLCM, SVM and FCM
Authors: Li Bo , Xu Jin , Pan Xinxiang , Ma Long , Zhao Zhiqiang , Chen Rong , Liu Qiao , Wang Haixia
Published: August 2022

Marine oil spills have a significant adverse impact on the economy, ecology, and human health. Rapid and effective oil spill monitoring action is extraordinarily important for controlling marine pollution. A marine oil spill detection scheme based on X-band shipborne radar image with machine learning is proposed here. First, the original shipborne radar image collected on Dalian 7.16 oil spill accident was transformed into a Cartesian coordinate system and noise suppressed. Then, texture features and SVM were used to indicate the effective monitoring location of ocean waves. Third, FCM was applied to classify the oil films and ocean waves. Finally, the oil spill detection result was transformed back to a polar coordinate system. Compared with an improved active contour model and another oil spill detection method with SVM, our method performed more intelligently. It can provide data support for marine oil spill emergency response.
Effects of Low Temperature on the Relationship between Solar-Induced Chlorophyll Fluorescence and Gross Primary Productivity across Different Plant Function Types
Authors: Chen Jidai , Liu Xinjie , Ma Yan , Liu Liangyun
Published: August 2022

Solar-induced chlorophyll fluorescence (SIF) has been recognized as a proxy of gross primary production (GPP) across various terrestrial biomes. However, the effects of low temperature on SIF and GPP among different plant function types (PFTs) have not yet been well-explored. To gain a better understanding of the relationship between SIF and GPP, we investigated the variation in the GPP/SIF ratio in response to low-temperature conditions using satellite and tower-based datasets. Based on the TROPOMI SIF product and FLUXCOM GPP data, we found that the SIF and GPP exhibited consistent seasonal and spatial patterns, while the GPP/SIF ratio differed for different PFTs. The GPP/SIF ratio for forest types was generally higher than 10 gC·d−1·mw−1·nm·sr, whereas the GPP/SIF ratio for grass and crop types was generally lower than 10 gC·d−1·mw−1·nm·sr. In addition, there were noticeable differences in the seasonal pattern of the GPP/SIF ratio between the selected samples that experienced low-temperature stress (below 10 °C, defined as group A) and those that grew under relatively warm conditions (above 10 °C throughout the year, defined as group B). The GPP/SIF ratio for group A generally exhibited a “hump-shaped” seasonal pattern, and that for group B showed a slightly “bowl-shaped” seasonal pattern, which means it is important to consider the effects of temperature on the SIF-GPP relationship. Through linear regression and correlation analysis, we demonstrate that there was a positive correlation between the GPP/SIF ratio and temperature for group A, with a wide temperature range including low-temperature conditions, indicating that, in this case, temperature affected the SIF–GPP relationship; however, for group B—with a temperature higher than 10 °C throughout the year—the GPP/SIF ratio was not consistently affected by temperature. The response of GPP/SIF to low temperature stress was confirmed by tower-based observations at a C3 cropland (C3CRO) site and a boreal evergreen needleleaf forest (BoENF) site. Although the relationship between the GPP/SIF ratio and temperature differed among PFTs, the GPP/SIF ratio decreased under low-temperature conditions for PFTs. Therefore, the GPP/SIF ratio was not constant and was largely influenced by low temperature for different PFTs, thus highlighting the importance of incorporating temperature into SIF-based GPP estimation.