bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization and ontogeny of a novel lymphoid follicle inducer cell during development of the bursa of Fabricius
Authors: Szőcs Emőke , Balic Adam , Soós Ádám , Halasy Viktória , Nagy Nándor
Published: October 2024

The avian bursa of Fabricius (BF) is a primary lymphoid organ, where B-cell development occurs within bursal follicles of epithelial origin. During embryogenesis the epithelial anlage of the BF emerges as a diverticulum of the cloaca surrounded by undifferentiated tail bud mesenchyme. While it is believed that the epithelial-mesenchymal BF primordium provides a selective microenvironment for developing B cells, the initial events inducing lymphoid follicle formation are not fully elucidated. Using wild type and CSF1R-eGFP transgenic chick embryos, we find that separate B cell, macrophage and dendritic cell precursors enter the BF mesenchyme, migrate to the surface epithelium, and colonize the lymphoid follicle buds. Detailed immunocytochemical characterization revealed a novel EIV-E12+ blood-borne cell type, colonizing the surface epithelium of the BF rudiment before the entry of myeloid and lymphoid lineages and the appearance of this cell type coincides with the onset of follicle bud formation. Chick-duck chimeras and chick-quail tissue recombination experiments suggest that EIV-E12+ cells represent a transient lymphoid inducer cell population. They are not dendritic or B cells precursors, and they are capable of follicle bud induction in both dendritic cell- and B cell-depleted bursae.
Temporal niche partitioning as a potential mechanism for coexistence in two sympatric mesopredator sharks
Authors: Cook Neil D. , Jenkins Abbie , Perry Sarah L. , Perkins Sarah E. , Cable Jo
Published: October 2024

Global marine biodiversity declines require bold and ambitious plans to safeguard ecosystem services. Overfishing, habitat loss and projected climate impacts have yielded deleterious effects on marine predators, in particular, driving increasing threat of extinction for many shark species with implications for ecosystem health. Identifying and protecting critical habitat areas for sharks is fundamental to their protection, and may allow for species recovery. Here we use baited remote underwater video stations to investigate spatial and temporal patterns of habitat use by sharks in a Special Area of Conservation (SAC) that is centrally important to the regional blue economy in the UK, the Cardigan Bay SAC. Our results show heterogeneous spatial habitat use and temporal trends in habitat sharing between smallspotted catsharks Scyliorhinus canicula and nursehounds S. stellaris. Nearshore reef habitats are substantially more important than non-reef habitats for both species. The relative abundance of each species, however, is roughly inversely seasonally proportional, with S. canicula and S. stellaris relative abundance highest in March and September, respectively. Temporal niche partitioning may be an important mechanism in marine systems, but has not been widely investigated in sympatric shark communities. These findings are directly relevant for ongoing development of the Welsh Marine Evidence Strategy (2019-2025) and the Welsh National Marine Plan, particularly to inform spatial planning to strengthen the role of SAC management design in protecting important shark areas.
Rapid and precise identification of cervicothoracic necrotizing fasciitis caused by Prevotella and Streptococcus constellatus by using Nanopore sequencing technology: a case report
Authors: Zhao Manna , Leng Xuejun , Xu Jie , Cui Juanjuan , Li Shuo , Zhao Weifeng
Published: October 2024

Introduction

Cervicothoracic necrotizing fasciitis (CNF) is one form of necrotizing soft-tissue infections, which could lead to patient demise during short course. Therefore, early recognition and immediate treatment contribute to promising prognosis of patients.

Case presentation

A 58-year-old diabetic patient presented with a sore throat and progressive irritation of the neck and chest for 4 days. The initial diagnosis was considered to be soft-tissue infection and the clinician gave empirical anti-infectious medication for expectant treatment. During the course of disease, surgical incision was performed to relieve suffocation and shortness of breath. The drainage fluids were detected with microbiological culture and molecular sequencing. Nanopore sequencing technology (NST) helped to identify the coinfection of Streptococcus constellatus and Prevotella spp., which was not recognized during the original period of 15 days. The precise identification of pathogen supported to guide the pharmacologic treatment with meropenem and linezolid. Ultimately, combined with the surgical observation and post-surgical pathological examination, the patient was diagnosed as CNF, which could be much more acute and serious than normal soft-tissue infections. The patient has been successfully treated with prompt antimicrobial medication and appropriate surgical debridement.

Conclusion

This case presented a CNF patient with type 2 diabetes, successfully recovered after prompt microbial detection, precise anti-infectious treatment, and appropriate surgical intervention. It highlights the importance of recognizing pathogen by applying rapid microbiological detection, including NST, in acute and serious infectious disease.
The progress of Mycobacterium tuberculosis drug targets
Authors: Zhang Xin , Zhao Ruixia , Qi Yao , Yan Xiong , Qi Gaoxiu , Peng Qiuju
Published: October 2024

Tuberculosis (TB) has been troubling humans for hundreds of years, is a highly infectious disease caused by Mycobacterium tuberculosis (Mtb) infection, Mtb can infect almost all organs of the body and is one of the deadly infectious diseases in the world. At present, the first-line treatment regimen has a long treatment cycle and is prone to multiple drug resistance. Anti-tuberculosis drugs and latent tuberculosis infection (LTBI) resistance are increasing year by year, and new targets and new bioactive compounds are urgently needed to treat this disease. This review focuses on the latest reported anti-TB drug targets and related compounds in recent years, reviews the current TB drug regimen and major defects, outlines the key drug targets developed to date in Mtb, and the current situation of newly discovered anti-TB resistant forms of drugs. To provide a reference for the research and development of new anti-TB drugs and bring new treatment strategies for TB patients.
Helicobacter pylori infection and inflammatory bowel disease: a 2-sample Mendelian randomization study
Authors: Cui Yurong , Li Jinxin , Zhao Bing , Liu Junying
Published: October 2024

Introduction

Observational studies have discovered a contradictory phenomenon between Helicobacter pylori (H. pylori) infection and inflammatory bowel disease (IBD). The study aimed to confirm the causal association between H. pylori and IBD, including ulcerative colitis (UC) and Crohn's disease (CD).

Methods

We conducted a Mendelian randomization (MR) study with two sample Genome-Wide Association Studies (GWAS) to determine whether there is a causal relationship between H. pylori infection and IBD, as well as the possible pathogenic factors that may be involved. The reliability of the main MR assumptions was examined through a series of sensitivity analyses.

Results

Two genetic variants (SNPs) previously identified were employed as instrumental variables (IVs) for H. pylori infection. GWAS data for IBD, UC, and CD were obtained from the recent DF10 release10 of the FinnGen study. Our findings indicated a significant association between H. pylori seropositivity and an increased risk of IBD and UC (IBD: OR: 1.16, 95% CI, 1.03–1.31, P < 0.05; UC: OR: 1.22, 95% CI, 1.08–1.37, P < 0.001) while no causal relationship with CD (P > 0.05). Analysis of the main virulence pathogenic factors revealed a causal relationship between cytotoxin-associated protein A (CagA) and IBD and UC (IBD: OR: 1. 06, 95% CI, 1.001–1.11, P < 0.05; UC: OR: 1.07, 95% CI, 1.004–1.14, P < 0.05), while no correlation was found for vacuolar cytotoxin A (VacA) (P > 0.05). After applying the False Discovery Rate (FDR) correction, the causal relationship between CagA and the risk of IBD or UC was no longer statistically significant.

Conclusion

This study suggests a potential causal relationship between H. pylori infection and IBD, particularly UC. The effect may be more pronounced in individuals with previous H. pylori infections.
A rapid point-of-care population-scale dipstick assay to identify and differentiate SARS-CoV-2 variants in COVID-19-positive patients
Authors: Paul Deepjyoti , Verma Jyoti , Kumar Shakti , Talukdar Daizee , Jana Pradipta , Narendrakumar Lekshmi , Kumar Roshan , Tanwar Subhash , Gosain Mudita , Porey Karmakar Sonali , Pareek Madhu , Mani Shailendra , Chaudhuri Susmita , Kshetrapal Pallavi , Wadhwa Nitya , Bhatnagar Shinjini , Garg Pramod Kumar , Das Bhabatosh
Published: October 2024

Delta and Omicron variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) are remarkably contagious, and have been recognized as variants of concern (VOC). The acquisition of spontaneous substitutions or insertion–deletion mutations (indels) in the spike protein-encoding gene substantially increases the binding affinity of the receptor binding domain (RBD)-hACE2 complex and upsurges the transmission of both variants. In this study, we analyzed thousands of genome sequences from 30 distinct SARS-CoV-2 variants, focusing on the unique nucleic acid signatures in the spike gene specific to the Delta and Omicron variants. Using these variant-specific sequences, we synthesized a range of oligonucleotides and optimized a multiplex PCR (mPCR) assay capable of accurately identifying and differentiating between the Delta and Omicron variants. Building on this mPCR assay, we developed a dipstick format by incorporating a tag linker sequence at the 5′ end of the forward primer and adding biotin to the 3′ end of the oligonucleotides, enhancing the assay’s usability and accessibility. Streptavidin-coated latex beads and the dipstick imprinted with a probe for the tag linker sequence in the test strips were used for the detection assay. Our dipstick-based assay, developed as a rapid point-of-care test for identifying and differentiating SARS-CoV-2 variants has the potential to be used in low-resource settings and scaled up to the population level.
The structure and function of rhizosphere bacterial communities: impact of chemical vs. bio-organic fertilizers on root disease, quality, and yield of Codonopsis pilosula
Authors: Huang Bin , Chen Yuxuan , Cao Yi , Liu Dongyang , Fang Hua , Zhou Changchun , Wang Dong , Wang Jie
Published: October 2024

Introduction

Long-term use of chemical fertilizers (CFs) can cause soil compaction and acidification. In recent years, bio-organic fertilizers (BOFs) have begun to replace CFs in some vegetables and cash crops, but the application of CFs or BOFs has resulted in crop quality and disease occurrence.

Methods

This study aimed to analyze the microbial mechanism of differences between CFs and BOFs in root disease, quality, and yield of tuber Chinese herbal medicine. We studied the effects of CFs, organic fertilizers, commercial BOFs, biocontrol bacteria BOFs, and biocontrol fungi BOFs on rhizosphere microbial community structure and function, root rot, quality, and yield of Codonopsis pilosula at different periods after application and analyzed the correlation.

Results and discussion

Compared to CFs, the emergence rate and yield in BOF treatments were increased by 21.12 and 33.65%, respectively, and the ash content, water content, and disease index in the BOF treatments were decreased by 17.87, 8.19, and 76.60%, respectively. The structural equation model showed that CFs promoted the quality and yield of C. pilosula by influencing soil environmental factors, while BOFs directly drove soil bacterial community to reduce disease index and improve the quality and yield of C. pilosula. There was a stronger interaction and stability of soil microbial networks after BOF treatments. Microlunatus, Rubrobacter, Luteitalea, Nakamurella, and Pedomicrobium were identified as effector bacteria, which were related to disease prevention and yield and quality increase of C. pilosula. Microbial functional analysis indicated that the signal transduction and amino acid metabolism of soil bacteria might play a major role in improving the quality and yield of C. pilosula in the early and middle growth stages. In conclusion, compared to CFs, BOFs obtained a lower disease index of root rot and a higher quality and yield of C. pilosula by changing the structure and function of the rhizosphere bacterial community.
Comparative analysis of the leprosy detection rate regarding its clinical spectrum through PCR using the 16S rRNA gene: a scientometrics and meta-analysis
Authors: Silva Marcos Jessé Abrahão , Brasil Thiago Pinto , Silva Caroliny Soares , Frota Cristiane Cunha , Sardinha Daniele Melo , Figueira Luiza Raquel Tapajós , Neves Keitty Anne Silva , dos Santos Everaldina Cordeiro , Lima Karla Valéria Batista , Ghisi Nédia de Castilhos , Lima Luana Nepomuceno Gondim Costa
Published: October 2024

Background

Leprosy is a chronic and disabling infectious disease caused by Mycobacterium leprae. It has a wide clinical spectrum and is operationally classified into paucibacillary (PB) and multibacillary (MB) cases. There is evidence that the 16S rRNA gene can be used in Polymerase Chain Reaction (PCR) for complementary detection with high sensitivity and specificity. However, there is no literature convention on its diagnostic correspondence regarding the particular operational classification of the disease. This study aimed to correlate, through a meta-analysis, the detection rate of leprosy between the PCR method with the 16S rRNA gene in the clinical forms PB and MB in relation to confirmed cases.

Methods

This is a systematic review and meta-analysis study conducted according to the PRISMA 2020 guidelines, using the search descriptors with “AND”: “Leprosy”; “Polymerase Chain Reaction”; “16S rRNA” in the PUBMED, SciELO, LILACS, and Science Direct databases. The search was limited to original observational articles in Portuguese, English, or Spanish, with no defined time frame. The methodological quality assessment of the selected articles was performed using the JBI checklists. A scientometric approach to the article using used the VOS Viewer and Scimago Graphica software. The meta-analysis was conducted using Comprehensive Meta-Analyses software, under Pearson’s Correlation effect test and fixed effect model and subgroup analysis concerning the type of sample analyzed.

Results

The study was significant from the perspective of the paucibacillary group (Clinical biopsy: -0.45 [95% CI= -0.63 – −0.22], p < 0.001/ Slit smear skin: −0.52 [95% CI= -0.65 – −0.36], p < 0.001 / Overall: −0.50 [95% CI= −0.61 – −0.37], p < 0.001). The PCR diagnostic method for the16S rRNAgene ofM. lepraehas low viability and diagnostic sensitivity in both clinical biopsy samples and leprosy skin smears.

Conclusion

This implies little validation of it as a PCR target gene for diagnosing the disease, highlighting limitations in the actual technique.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42024588790.
TL-CStrans Net: a vision robot for table tennis player action recognition driven via CS-Transformer
Authors: Ma Libo , Tong Yan
Published: October 2024

Currently, the application of robotics technology in sports training and competitions is rapidly increasing. Traditional methods mainly rely on image or video data, neglecting the effective utilization of textual information. To address this issue, we propose: TL-CStrans Net: A vision robot for table tennis player action recognition driven via CS-Transformer. This is a multimodal approach that combines CS-Transformer, CLIP, and transfer learning techniques to effectively integrate visual and textual information. Firstly, we employ the CS-Transformer model as the neural computing backbone. By utilizing the CS-Transformer, we can effectively process visual information extracted from table tennis game scenes, enabling accurate stroke recognition. Then, we introduce the CLIP model, which combines computer vision and natural language processing. CLIP allows us to jointly learn representations of images and text, thereby aligning the visual and textual modalities. Finally, to reduce training and computational requirements, we leverage pre-trained CS-Transformer and CLIP models through transfer learning, which have already acquired knowledge from relevant domains, and apply them to table tennis stroke recognition tasks. Experimental results demonstrate the outstanding performance of TL-CStrans Net in table tennis stroke recognition. Our research is of significant importance in promoting the application of multimodal robotics technology in the field of sports and bridging the gap between neural computing, computer vision, and neuroscience.
The role of synaptic protein NSF in the development and progression of neurological diseases
Authors: Yang Jingyue , Kong Lingyue , Zou Li , Liu Yumin
Published: October 2024

This document provides a comprehensive examination of the pivotal function of the N-ethylmaleimide-sensitive factor (NSF) protein in synaptic function. The NSF protein directly participates in critical biological processes, including the cyclic movement of synaptic vesicles (SVs) between exocytosis and endocytosis, the release and transmission of neurotransmitters, and the development of synaptic plasticity through interactions with various proteins, such as SNARE proteins and neurotransmitter receptors. This review also described the multiple functions of NSF in intracellular membrane fusion events and its close associations with several neurological disorders, such as Parkinson’s disease, Alzheimer’s disease, and epilepsy. Subsequent studies should concentrate on determining high-resolution structures of NSF in different domains, identifying its specific alterations in various diseases, and screening small molecule regulators of NSF from multiple perspectives. These research endeavors aim to reveal new therapeutic targets associated with the biological functions of NSF and disease mechanisms.
Multi-level feature fusion network for neuronal morphology classification
Authors: Sun Chunli , Zhao Feng
Published: October 2024

Neuronal morphology can be represented using various feature representations, such as hand-crafted morphometrics and deep features. These features are complementary to each other, contributing to improving performance. However, existing classification methods only utilize a single feature representation or simply concatenate different features without fully considering their complementarity. Therefore, their performance is limited and can be further improved. In this paper, we propose a multi-level feature fusion network that fully utilizes diverse feature representations and their complementarity to effectively describe neuronal morphology and improve performance. Specifically, we devise a Multi-Level Fusion Module (MLFM) and incorporate it into each feature extraction block. It can facilitate the interaction between different features and achieve effective feature fusion at multiple levels. The MLFM comprises a channel attention-based Feature Enhancement Module (FEM) and a cross-attention-based Feature Interaction Module (FIM). The FEM is used to enhance robust morphological feature presentations, while the FIM mines and propagates complementary information across different feature presentations. In this way, our feature fusion network ultimately yields a more distinctive neuronal morphology descriptor that can effectively characterize neurons than any singular morphological representation. Experimental results show that our method effectively depicts neuronal morphology and correctly classifies 10-type neurons on the NeuronMorpho-10 dataset with an accuracy of 95.18%, outperforming other approaches. Moreover, our method performs well on the NeuronMorpho-12 and NeuronMorpho-17 datasets and possesses good generalization.
A real-world retrospective study to assess efficacy and safety of alectinib as adjuvant therapy in IB-IIIB NSCLC patients harboring ALK rearrangement
Authors: Chang Zeng-Hao , Zhu Teng-Fei , Ou Wei , Jiang Hao , Wang Si-Yu
Published: October 2024

Background

Alectinib has demonstrated promising disease-free survival (DFS) benefit for early-stage non-small cell lung cancer (NSCLC) patients with ALK rearrangement positive in phase 3 ALINA trial. However, real-world evidence for the efficacy and safety of alectinib in early-stage ALK-positive NSCLC is limited.

Materials and methods

We retrospectively reviewed 68 patients with stage IB-IIIB ALK-positive NSCLC who underwent complete pulmonary resections from April 2010 to July 2023 at a single institution. 38 (55.9%) enrolled patients had N2 lymph node metastasis, and 17 (24.9%) patients had multi-station N2 metastasis. Patients were stratified into two groups according to the adjuvant treatment regimen, with 19 patients in the alectinib group and 49 patients in the chemotherapy group. There were no significant differences in clinicopathological characteristics between the two groups. After curative resection surgery, patients in alectinib group received oral alectinib at a dose of 600 mg twice daily and patients in chemotherapy group received platinum-based doublet chemotherapy regimen every 3 weeks for 4 cycles. The primary endpoint was 3-year DFS. The Kaplan-Meier method was used to estimate DFS and overall survival (OS). Safety analyses were conducted by comparing the incidence of adverse events between the two groups.

Results

At the last follow-up date (January 22th, 2024), A total of 1 (5.3%) and 28 (57.1%) DFS events were observed in alectinib group and chemotherapy group respectively. The 3-year DFS showed significant improvement in the alectinib group compared with chemotherapy group (91.7% vs 60.7%, P=0.051). In the IIIAN2 subgroup, the 3-year DFS rate in the alectinib group reached a satisfactory 87.5%. In both groups, the majority of AEs were graded as level 1 or 2, No grade 3-4 AEs were observed in alectinib group.

Conclusion

Alectinib, as adjuvant therapy, demonstrated favorable efficacy and manageable safety in patients with completely resected ALK-positive stage I B-IIIB non-small cell lung cancer. A limitation of this study is the small sample size, and a larger-scale real-world sample study is needed to further evaluate the efficacy and safety of alectinib as adjuvant therapy.
Deciphering the molecular heterogeneity of intermediate- and (very-)high-risk non–muscle-invasive bladder cancer using multi-layered –omics studies
Authors: Akand Murat , Jatsenko Tatjana , Muilwijk Tim , Gevaert Thomas , Joniau Steven , Van der Aa Frank
Published: October 2024

Bladder cancer (BC) is the most common malignancy of the urinary tract. About 75% of all BC patients present with non-muscle-invasive BC (NMIBC), of which up to 70% will recur, and 15% will progress in stage and grade. As the recurrence and progression rates of NMIBC are strongly associated with some clinical and pathological factors, several risk stratification models have been developed to individually predict the short- and long-term risks of disease recurrence and progression. The NMIBC patients are stratified into four risk groups as low-, intermediate-, high-risk, and very high-risk by the European Association of Urology (EAU). Significant heterogeneity in terms of oncological outcomes and prognosis has been observed among NMIBC patients within the same EAU risk group, which has been partly attributed to the intrinsic heterogeneity of BC at the molecular level. Currently, we have a poor understanding of how to distinguish intermediate- and (very-)high-risk NMIBC with poor outcomes from those with a more benign disease course and lack predictive/prognostic tools that can specifically stratify them according to their pathologic and molecular properties. There is an unmet need for developing a more accurate scoring system that considers the treatment they receive after TURBT to enable their better stratification for further follow-up regimens and treatment selection, based also on a better response prediction to the treatment. Based on these facts, by employing a multi-layered –omics (namely, genomics, epigenetics, transcriptomics, proteomics, lipidomics, metabolomics) and immunohistopathology approach, we hypothesize to decipher molecular heterogeneity of intermediate- and (very-)high-risk NMIBC and to better stratify the patients with this disease. A combination of different –omics will provide a more detailed and multi-dimensional characterization of the tumor and represent the broad spectrum of NMIBC phenotypes, which will help to decipher the molecular heterogeneity of intermediate- and (very-)high-risk NMIBC. We think that this combinatorial multi-omics approach has the potential to improve the prediction of recurrence and progression with higher precision and to develop a molecular feature-based algorithm for stratifying the patients properly and guiding their therapeutic interventions in a personalized manner.
Deciphering the potential role of post-translational modifications of histones in gastrointestinal cancers: a proteomics-based review with therapeutic challenges and opportunities
Authors: Farrokhi Yekta Reyhaneh , Farahani Masoumeh , Koushki Mehdi , Amiri-Dashatan Nasrin
Published: October 2024

Oncogenesis is a complex and multi-step process, controlled by several factors including epigenetic modifications. It is considered that histone modifications are critical components in the regulation of gene expression, protein functions, and molecular interactions. Dysregulated post-translationally modified histones and the related enzymatic systems are key players in the control of cell proliferation and differentiation, which are associated with the onset and progression of cancers. The most of traditional investigations on cancer have focused on mutations of oncogenes and tumor suppressor genes. However, increasing evidence indicates that epigenetics, especially histone post-translational modifications (PTMs) play important roles in various cancer types. Mass spectrometry-based proteomic approaches have demonstrated tremendous potential in PTMs profiling and quantitation in different biological systems. In this paper, we have made a proteomics-based review on the role of histone modifications involved in gastrointestinal cancers (GCs) tumorigenesis processes. These alterations function not only as diagnostic or prognostic biomarkers for GCs, but a deeper comprehension of the epigenetic regulation of GCs could facilitate the treatment of this prevalent malignancy through the creation of more effective targeted therapies.
Evaluating the impact of electrode planes on regional lung function assessment
Authors: Sang Ling , Sun Yutao , Lu Yu , Lin Zhimin
Published: October 2024

Objective

The aim of the study was to explore the influence of the measurement plane on regional lung function assessed via electrical impedance tomography (EIT).

Methods

The forced vital capacity (FVC) maneuver was prospectively performed in 30 healthy male volunteers. Simultaneously, EIT measurements were conducted at the 3rd, 4th, and 5th intercostal spaces (ICS). The EIT-based spirometry parameters are calculated in a similar manner to their original definitions. The spatial and temporal distributions of the corresponding functional images were assessed and compared among the measurement planes.

Results

All subjects but one were able to perform the FVC maneuver according to the guidelines. Significant differences were found in 67% (6 out of 9) of the EIT-based parameters assessing the spatial and temporal distribution. The fEIT images were most homogeneous at ICS 4 compared to the other two measurement planes, except for the time required for 75% of FVC. The fEIT image FVCEIT distributed toward dorsal regions when the measurement planes moved from ICS 3 to ICS 5, whereas the identified lung areas became smaller.

Conclusion

The spatial and temporal distribution of the regional lung function measured via EIT was influenced by the measurement planes. We recommend adhering to the same measurement plane for before–after comparison. ICS 4 was recommended for the sitting subjects performing lung function testing.
Limb dominance influences landing mechanics and neuromuscular control during drop vertical jump in patients with ACL reconstruction
Authors: Xue Boshi , Yang Xiaowei , Wang Xia , Yang Chen , Zhou Zhipeng
Published: October 2024

Objectives

The purpose of this study was to compare the interlimb biomechanical differences in patients who had undergone anterior cruciate ligament reconstruction (ACLR) in either dominant (ACLR-D) or nondominant (ACLR-ND) limbs and healthy controls (CON) during drop vertical jump (DVJ) task. To investigate whether the dominant or nondominant limb influences the risk of re-injury in ACLR patients.

Methods

Thirty-three ACLR patients were divided into ACLR-D and ACLR-ND groups according to whether the surgical limb was dominant or nondominant. Seventeen healthy individuals were selected as the CON group. Three-dimensional kinematic data, ground reaction force (GRF) data, and surface electromyographic (EMG) data from the bilateral lower limbs of all participants were collected during the DVJ task. Two-way repeated-measures ANOVAs (limb × group) were performed on the variables of interest to examine the main effects of limb (dominant vs. nondominant) and group (ACLR-D, ACLR-ND, and CON), as well as the interaction between limb and group.

Results

The nonsurgical limbs of ACLR group had significantly greater knee valgus angles, knee extension and valgus moments, peak posterior GRF (PPGRF), and peak vertical GRF (PVGRF) compared to the surgical limbs. The nonsurgical limbs of ACLR-ND patients demonstrated significantly greater knee extension and valgus moments, greater PPGRF and PVGRF, and reduced muscle activity in the vastus medialis and vastus lateralis compared to the CON group. The ACLR patients had reduced muscle activity in the quadriceps of the surgical limb and the hamstrings of the bilateral limbs compared to controls.

Conclusion

The nonsurgical limbs of ACLR patients may suffer an increased risk of ACL injury due to altered landing mechanics and neuromuscular control strategies compared to the surgical limbs. Additionally, limb dominance influences movement patterns and neuromuscular control during DVJ task, the nonsurgical limbs of the ACLR-ND might be at higher risk of ACL injury compared to the ACLR-D group.
Response of young rice panicles to salt stress: insights based on phenotype and transcriptome analysis
Authors: Duan Fanrui , Wu Fugui , Li Zhen , Zhang Kai , Ma Qilin
Published: October 2024

Saline-alkali soils limit rice growth and production. With an increasing global population, enhancing rice salt tolerance is crucial for improving yields in these areas. This study investigated the developmental characteristics of young panicles and pollen fertility in two rice varieties, 58M and 58L, under salt stress. Results showed that 58M had more substantial salt tolerance during panicle development. RNA sequencing of 18 samples from both varieties under high salt stress (0 h, 6 h, and 24 h) identified 469 common differentially expressed genes (DEGs) and 2,308 DEGs between the varieties. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment highlighted significant pathways such as phenylpropanoid biosynthesis, protein processing, and flavonoid biosynthesis. Six gene co-expression modules related to salt tolerance were identified, with six candidate genes (LOC_Os05g38530, LOC_Os04g07920, LOC_Os12g02105, LOC_Os01g06580, LOC_Os06g49250, and LOC_Os06g48300) potentially linked to salt tolerance. These findings provide insights into rice salt tolerance mechanisms and offer new genetic resources for breeding salt-tolerant rice.
Bioenergy sorghum nodal root bud development: morphometric, transcriptomic and gene regulatory network analysis
Authors: Lamb Austin , Kurtz Evan , Glenn Priscilla , McKinley Brian A. , Mullet John
Published: October 2024

Bioenergy sorghum’s large and deep nodal root system and associated microbiome enables uptake of water and nutrients from and deposition of soil organic carbon into soil profiles, key contributors to the crop’s resilience and sustainability. The goal of this study was to increase our understanding of bioenergy sorghum nodal root bud development. Sorghum nodal root bud initiation was first observed on the stem node of the 7th phytomer below the shoot apex. Buds were initiated near the upper end of the stem node pulvinus on the side of the stem opposite the tiller bud, then additional buds were added over the next 6-8 days forming a ring of 10-15 nascent nodal root buds around the stem. Later in plant development, a second ring of nodal root buds began forming on the 17th stem node immediately above the first ring of buds. Overall, nodal root bud development can take ~40 days from initiation to onset of nodal root outgrowth. Nodal root buds were initiated in close association with vascular bundles in the rind of the pulvinus. Stem tissue forming nascent nodal root buds expressed sorghum homologs of genes associated with root initiation (WOX4), auxin transport (LAX2, PIN4), meristem activation (NGAL2), and genes involved in cell proliferation. Expression of WOX11 and WOX5, genes involved in root stem niche formation, increased early in nodal root bud development followed by genes encoding PLTs, LBDs (LBD29), LRP1, SMB, RGF1 and root cap LEAs later in development. A nodal root bud gene regulatory network module expressed during nodal root bud initiation predicted connections linking PFA5, SPL9 and WOX4 to genes involved in hormone signaling, meristem activation, and cell proliferation. A network module expressed later in development predicted connections among SOMBRERO, a gene involved in root cap formation, and GATA19, BBM, LBD29 and RITF1/RGF1 signaling. Overall, this study provides a detailed description of bioenergy sorghum nodal root bud development and transcriptome information useful for understanding the regulation of sorghum nodal root bud formation and development.
The Reicher-Wheeler paradigm in word recognition research: a cautionary note on its actual contributions and published misconceptions
Authors: Jordan Timothy R. , Akkaya Aziz M. , Göçmüş Fatma Zehra , Kalan Aleynanur , Morgul Ebru , Önalan Kübra , Sheen Mercedes Kier
Published: October 2024

The study of word recognition has been influenced greatly by findings obtained when visual stimuli are presented very briefly. Under these conditions, a great deal of evidence suggests that words are perceived better than nonwords, and even single letters, and it is generally accepted that these “word superiority effects” reflect the relative efficiency with which words are perceived. For more than 50 years, a key procedure for establishing these effects has been the Reicher-Wheeler Paradigm in which potentially confounding effects of non-perceptual guesswork are cleverly suppressed. More recently, however, the actual nature of the Reicher-Wheeler paradigm and its contribution to research have become misrepresented in a range of publications, and its use in experiments has been confused and conflated with other, less sophisticated procedures. In this article we describe the actual contributions made by the Reicher-Wheeler Paradigm to word recognition research and show examples of how these important contributions have been misunderstood and misconceived in experiments reported in the recent literature.
The impact of immersive virtual reality meditation for depression and anxiety among inpatients with major depressive and generalized anxiety disorders
Authors: Lee Jungjoo , Kim Junhyoung , Ory Marcia G.
Published: October 2024

Background

Mindfulness-Based Cognitive Therapy (MBCT) is a non-pharmacological approach to alleviating depression and anxiety. While technology based MBCT is a standardized cost-effective approach, there have been concerns about its feasibility and effectiveness in clinical settings.

Aims

The purpose of this study was to investigate the longitudinal relationship between improved emotional regulation resulting from participation in Immersive Virtual Reality Meditation (IVRM) and Major Depressive Disorder (MDD) and Generalized Anxiety Disorder (GAD) as monitored by electrocardiogram.

Methods

This study was a longitudinal single-arm clinical trial in which the intervention was conducted three times a week for 10 weeks at a behavioral health unit in a community hospital (n = 26). We measured Coherence Achievement Score (CAS), depression, and anxiety. The relationships between CAS, anxiety, depression, and covariates were analyzed using a Generalized Estimated Equation (GEE).

Results

The findings of our study provide evidence that the CAS scores indicative of emotional regulation function after IVRM participation were associated with a reduction in depression and anxiety.

Conclusion

Among the many technology-based complementary health care interventions that are available to reduce depression and anxiety, IVRM program use increases emotional regulatory function and decrease depression and anxiety.
Tendencies and characteristics of public fitness behavior in the digital era: an Internet ethnography study based on the K Community in China
Authors: Jia Chen , Ye Songdong
Published: October 2024

Introduction

The deep integration of media technologies into fitness activities in the era of digital intelligence has created a new and evolving landscape for public fitness. This transformation significantly impacts the practices and experiences of fitness.

Methods

This study employs Internet ethnography as a qualitative research methodology, focusing on the daily activities of participants in a digital fitness community known as the K-community. Through immersive observation and analysis, the research seeks to uncover the evolving patterns and characteristics of fitness practices in China during the digital era.

Results

As media technology and network culture advance, participants in digital fitness increasingly display fragmented, digitized, entertaining, ritualized, and highly socialized behaviors. These traits reflect the community’s desire for varied and complex exercise experiences within the context of modern technology.

Discussion

The evolution of fitness behavior not only reflects the operational principles of contemporary media technologies but also encapsulates the essence of popular culture in the digital age. This study enhances our comprehension of fitness practices in digital environments and their wider cultural ramifications.
Peer bonds and nature’s embrace: exploring the influence of pet caregiving on social well-being and nature connection among Taiwanese children
Authors: Tseng Tzuhui Angie , Fang Hsiao-Yen , Shen Ching-Cheng , Chang Yun-Chen
Published: October 2024

This study investigated the associations between pet-ownership on nature engagement, interpersonal relationships, and well-being among 471 Taiwanese children aged 11–12, across different genders. The findings revealed that interactions with pets, primarily in the form of caregiving, enhanced empathy toward nature, notably among female participants from various racial and ethnic backgrounds, although direct contact with nature was generally limited. In terms of interpersonal relationships, family bonds were significant, with boys reporting stronger connections. Children who had previously or were currently owning pets displayed increased empathy toward the natural environment. The study highlighted a significant predictive relationship between children’s connection to nature and their development of interpersonal relationships, with peer relationships being particularly influential in predicting children’s well-being. These results underscored the importance of pet caregiving and peer interactions in the emotional and social development of children.
Seroprevalence of Hepatitis B virus surface antigen among African blood donors: a systematic review and meta-analysis
Authors: Quintas Angelina Edna , Cuboia Nelson , Cordeiro Lemuel , Sarmento António , Azevedo Luís
Published: October 2024

Background

Transfusion Transmitted Infections (TTIs) are still a growing public health problem in Africa. Studies that synthesize the available evidence on the seroprevalence of Hepatitis B Surface Antigen (HBsAg) among African blood donors are scarce. Therefore, this study aimed to synthesize qualitatively and quantitatively the seroprevalence of Hepatitis B Virus Surface Antigen (HBsAg) among blood donors in Africa.

Methods

We conducted a systematic review and meta-analysis where we included all studies that reported the seroprevalence of HBsAg among blood donors in Africa. The references were searched from electronic databases: PubMed, Web of Science, Cochrane, Scopus, WHO research database-HINARI, Global Index Medicus and ClinicalTrials.gov. We further analyzed the full list of references of all included studies. The pooled seroprevalence was estimated through random effect model. The heterogeneity was assessed through Cochrane’s Q test and I2, respectively. Meta-regression, subgroup and sensitivity analyses were conducted.

Results

We obtained 124 studies that met our inclusion criteria, comprising 3,573,211 blood donors tested for HBsAg. The pooled seroprevalence of HBsAg among blood donors in Africa was 6.93% (95% CI: 5.95–7.97%; I2 = 100%; p < 0.001). We found that the heterogeneity was explained by the study performed country and, African region. The higher prevalence was observed in Western 10.09% (95% CI: 8.75–11.50%), Central 7.81% (95% CI: 5.34–10.71%), and Eastern African region 4.87% (95% CI: 3.77–6.11%) and lower prevalence were observed in Southern 2.47% (95% CI: 0.54–5.75%) followed by Northern Africa region with 1.73% (95% CI: 0.45–3.79%). Additionally, based on the date of publication, we found that the highest prevalence was observed in studies published between 2001 and 2010 (9.41, 95% CI: 7.19–11.90) and the lowest prevalence was observed in studies published between 2011 and 2024 (6.26%; 95% CI: 5.19–7.42).

Conclusion

The seroprevalence of HBsAg among blood donors in Africa is still very high and heterogeneous. Therefore, intensifying the screening and vaccination of the population for Hepatitis B is critical to ensure blood safety toward eliminating Hepatitis B in Africa.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=395616, PROSPERO CRD42023395616.
Impact of hearing rehabilitation programs on presbycusis management: a systematic review and meta-analysis of randomized controlled trials
Authors: Zheng Zhanhang , Qin Shuhong , Li Ruilin , Wang Wenjuan , Wu Chenxingzi
Published: October 2024

Background

In the field of audiology, numerous studies have sought to understand and improve hearing rehabilitation programs for older adults afflicted with presbycusis. Despite this, the field lacks uniform standards pertaining to the intervention methods, frequency, and duration of such programs. These discrepancies have led to varying test results and inconsistent findings across multiple studies.

Objective

This meta-analysis aimed to evaluate the efficacy of hearing rehabilitation programs in enhancing the utilization of hearing aids among older adults with presbycusis.

Methods

We conducted a comprehensive exploration of PubMed, Embase, Cochrane Library, and Web of Science to identify randomized controlled trials assessing the role of hearing rehabilitation programs for patients with age-related hearing loss. The search period spanned from the inception of each database to September 12, 2024. Outcomes were synthesized using RevMan 5.4 software.

Results

Eight studies met the inclusion criteria, involving 598 patients (290 in the intervention group and 308 in the control group). It was observed that hearing rehabilitation programs significantly diminished self-perceived hearing impairment [MD = −5.80, 95% CI = (−8.16, −3.44), p < 0.00001] and negative emotional states [MD = −1.66, 95% CI = (−3.02, −0.29), p = 0.02], while enhancing hearing aid utilization [MD = 0.22, 95% CI = (0.08, 0.36), p = 0.002]. Nonetheless, these programs did not significantly augment patients’ satisfaction with their hearing aids [MD = 0.09, 95% CI = (−0.17, 0.26), p = 0.66].

Conclusion

Hearing rehabilitation programs significantly improve hearing aid outcomes, reduce self-perceived hearing impairment, and alleviate negative emotional states in patients. However, the current body of evidence is insufficient to conclusively indicate that these programs enhance patient satisfaction with daily hearing aid usage.
Coach perspectives on coach-athlete relationships and characteristics of Generation Z academy level rugby union players from South Africa
Authors: Landman Marc , Grobbelaar Heinrich , Kraak Wilbur
Published: October 2024

Introduction

The coach-athlete relationship is not merely a by-product of the coaching process but serves as its foundation. Coaches today must align their approaches with the characteristics (character, strengths, and growth areas) of a new generation of athletes, commonly referred to as Generation Z (Gen Z). Born between 1995 and 2012, Gen Z's grew up in a digital age, which shaped their character and behaviour. The purpose of the study was to explore coach-athlete relationships, the characteristics of Gen Z athletes that influence this relationship, and the process of building healthy coach-athlete relationships, from the perspectives of coaches.

Methods

The study used a qualitative methodology to elicit the perspectives of 11 male rugby union coaches (M ± SD age: 42.09 ± 7.56 years; with 12.83 ± 3.48 years of coaching experience), through semi-structured individual online interviews. We developed the interview script from Jowett's Coach-Athlete Relationship Questionnaire (CART-Q). The interviews were analysed using Merriam and Tisdell's six-step process.

Findings

The analysis yielded six super themes (and 17 themes). 1. Coach perspectives on coaching Gen Z players (developmental facilitators and debilitators, Covid-19's effect on development). 2. Closeness between coaches and athletes [loving and caring, getting closer, trusting, (not) respecting some players]. 3. Commitment (personally and towards the team, sacrifices). 4. Complementarity (goal setting, collectively working towards common goals). 5. Co-orientation (communicating, reciprocal relationship views). 6. Developing the coach-athlete relationship (approaches thereto, practical steps).

Discussion

Various facets of the coach-athlete relationship are interlinked and shaped by the characteristics and behaviours of this generation of athletes. Practical recommendations are offered on how to cultivate the coach-athlete relationship on emotional, cognitive, and behavioural levels.