bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence and Progression of Incidental Terminal Ileitis on Non-diagnostic Colonoscopy: A Systematic Review and Meta-analysis
Authors: Agrawal Manasi , Miranda Mario Bento- , Walsh Samantha , Narula Neeraj , Colombel Jean-Frederic , Ungaro Ryan C
Published: February 2021

Background

Incidentally diagnosed terminal ileitis [IDTI] has been reported among asymptomatic persons undergoing non-diagnostic colonoscopy. The purpose of our study was to determine the prevalence and long-term outcomes of asymptomatic terminal ileitis.

Methods

We performed a systematic review using three biomedical databases [Medline, Embase, and Web of Science] and relevant scientific meeting abstracts. We identified observational studies that reported the prevalence of IDTI in adults undergoing screening or polyp surveillance colonoscopy and/or the long-term outcomes of such lesions. A random-effects meta-analysis was conducted to determine the pooled prevalence rate of IDTI. The progression of IDTI to overt Crohn’s disease [CD] was also described.

Results

Of 2388 eligible studies, 1784 were screened after excluding duplicates, 84 were reviewed in full text, and 14 studies were eligible for inclusion. Seven studies reported the prevalence of IDTI in 44 398 persons undergoing non-diagnostic colonoscopy, six studies reported follow-up data, and one study reported both types of data. The pooled prevalence rate of IDTI was 1.6% (95% confidence interval [CI] 0.1–21.8%) with significant heterogeneity [I2 = 99.7]. Among patients who had undergone non-diagnostic colonoscopy and had follow-up data [range 13–84 months reported in five studies], progression to overt CD was rare.

Conclusions

IDTI is not uncommon on non-diagnostic colonoscopies. Based on limited data, the rate of its progression to overt CD seems low, and watchful waiting is likely a reasonable strategy. Further long-term follow-up studies are needed to inform the natural history of incidental terminal ileitis, factors that predict progression to CD, and therapeutic implications.
The Eclosion of Rhagoletis pomonella (Diptera: Tephritidae) Under Different Chill Durations and Simulated Temperate and Tropical Conditions
Authors: Neven Lisa G , Wakie Tewodros , Yee Wee L
Published: February 2021

The apple maggot fly, Rhagoletis pomonella (Walsh) (Diptera: Tephritidae), is a serious pest of apple in North America that is subject to quarantine measures to prevent its spread to currently pest-free regions, including the tropics. How the fly may survive in warmer climates is unclear. Here, we studied the effects of exposing postchill puparia to simulated temperate and tropical environmental conditions on eclosion of R. pomonella from Washington State, U.S.A. Puparia were chilled for 0–30 wk at 3°C and then held under four postchill conditions: A = 23°C, 16:8 L:D, 40% RH; B = 26°C, 12:12 L:D, 80% RH; C = 26°C, 16:8 L:D, 80% RH; and D = 23°C, 12:12 L:D, 40% RH, with B and D representing tropical conditions and A and C temperate conditions. Within each chill duration, total numbers of flies eclosed were equally high in tropical treatment B and temperate treatment C, while they were lower in treatments A and D. Mean weeks of the first eclosion in treatments B and C were earlier than in treatment D; mean week of peak eclosion and 50% eclosion in treatments A, B, and C were earlier than in treatment D. Eclosion spans in treatments A, B, and D were generally shorter than in treatment C. Results suggest that if introduced into a humid tropical country, R. pomonella puparia from Washington State could produce adult flies, regardless of chill duration or lack of chilling during the pupal stage, but whether flies could establish there would require further study.
Age- and sex-based normal values of layer-specific longitudinal and circumferential strain by speckle tracking echocardiography: the Copenhagen City Heart Study
Authors: Skaarup Kristoffer Grundtvig , Lassen Mats Christian Højbjerg , Johansen Niklas Dyrby , Olsen Flemming Javier , Lind Jannie Nørgaard , Jørgensen Peter Godsk , Jensen Gorm , Schnohr Peter , Prescott Eva , Søgaard Peter , Møgelvang Rasmus , Biering-Sørensen Tor
Published: February 2021

Aims

Technical advancements in 2D-speckle tracking echocardiography (2DSTE) have allowed for quantification of layer-specific global longitudinal strain (GLS) and circumferential strain (GCS) of the left ventricle (LV). The aim of this study was to establish age- and sex-based reference ranges of peak systolic layer-specific GLS and GCS and to assess normal values of regional strain.

Methods and results

We performed 2DSTE analysis of 1997 members of the general population from the fifth round of the Copenhagen City Heart Study, who were free of cardiovascular disease and risk factors. The mean age was 46 ± 16 years (range 21–97) and 62% were female. Mean values for peak systolic whole wall GLS (GLSWW.Sys), endomycardial (GLSEndo.Sys), and epimyocardial (GLSEpi.Sys) were 19.9 ± 2.1% (prediction interval [PI]: 15.8–24.0%), 23.5 ± 2.5% (PI: 18.6–28.4%), and 17.3 ± 1.9% (PI: 13.6–21.1%), respectively. Mean peak systolic whole wall GCS (GCSWW.Sys), was 21.6 ± 3.7% (PI: 14.3–28.9%), endomyocardial (GCSEndo.Sys) was 31.9 ± 4.7% (PI: 22.7–41.1%), and epimyocardial (GCSEpi.Sys) was 14.3 ± 3.8% (PI: 6.8–21.8%). A significant discrepancy in normal strain values between males and females was observed. Men had lower mean values and lower reference limits for all strain parameters. Furthermore, GLS and GCS changed differently with age in males and females. Finally, regional LS decreased from the apical to the basal LV region in both sexes, and regional CS varied significantly by LV segment.

Conclusion

In this study, we reported age- and sex-based reference ranges of layer-specific GLS and GCS. These reference ranges varied significantly with sex and age.
Clinical impact of PCSK9 inhibitor on stabilization and regression of lipid-rich coronary plaques: a near-infrared spectroscopy study
Authors: Ota Hideaki , Omori Hiroyuki , Kawasaki Masanori , Hirakawa Akihiro , Matsuo Hitoshi
Published: February 2021

Aims

This study aimed to determine the effects of a proprotein convertase subtilisin-kexin type 9 inhibitor (PCSK9i) on coronary plaque volume and lipid components in patients with a history of coronary artery disease (CAD).

Methods and results

This prospective, open-label, single-centre study analysed non-culprit coronary segments using near-infrared spectroscopy-intravascular ultrasound (NIRS-IVUS) at baseline and follow-up angiography. Following changes in the lipid-lowering treatment based on the most recent guideline, the enrolled subjects were divided into two groups: treatment with PCSK9i and statins (PCSK9i: 21 patients and 40 segments) and statins only (control: 32 patients and 50 segments). The absolute and percent LDL-C reductions were significantly greater in the PCSK9i group than in the control group (between group difference: 59.3 mg/dL and 46.4%; P < 0.001 for both). The percent reduction in normalized atheroma volume and absolute reduction in percent atheroma volume (PAV) were also significantly greater in the PCSK9i group (P < 0.001 for both). Furthermore, the PCSK9i group showed greater regression of maximal lipid core burden index for each of the 4-mm segments (maxLCBI4mm) than the control group (57.0 vs. 25.5; P = 0.010). A significant linear correlation was found between the percent changes in LDL-C and maxLCBI4mm (r = 0.318; P = 0.002), alongside the reduction in PAV (r = 0.386; P < 0.001).

Conclusion

The lipid component of non-culprit coronary plaques was significantly decreased by PCSK9i. The effects of statin combined with PCSK9i might be attributed to the stabilization and regression of residual vulnerable coronary plaques in patients with CAD.
Artificial intelligence to diagnose paroxysmal supraventricular tachycardia using electrocardiography during normal sinus rhythm
Authors: Jo Yong-Yeon , Kwon Joon-Myoung , Jeon Ki-Hyun , Cho Yong-Hyeon , Shin Jae-Hyun , Lee Yoon-Ji , Jung Min-Seung , Ban Jang-Hyeon , Kim Kyung-Hee , Lee Soo Youn , Park Jinsik , Oh Byung-Hee
Published: February 2021

Aims

Paroxysmal supraventricular tachycardia (PSVT) is not detected owing to its paroxysmal nature, but it is associated with the risk of cardiovascular disease and worsens the patient quality of life. A deep learning model (DLM) was developed and validated to identify patients with PSVT during normal sinus rhythm in this multicentre retrospective study.

Methods and results

This study included 12 955 patients with normal sinus rhythm, confirmed by a cardiologist. A DLM was developed using 31 147 electrocardiograms (ECGs) of 9069 patients from one hospital. We conducted an accuracy test with 13 753 ECGs of 3886 patients from another hospital. The DLM was developed based on residual neural network. Digitally stored ECG were used as predictor variables and the outcome of the study was ability of the DLM to identify patients with PSVT using an ECG during sinus rhythm. We employed a sensitivity map method to identify an ECG region that had a significant effect on developing PSVT. During accuracy test, the area under the receiver operating characteristic curve of a DLM using a 12-lead ECG for identifying PSVT patients during sinus rhythm was 0.966 (0.948–0.984). The accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of DLM were 0.970, 0.868, 0.972, 0.255, and 0.998, respectively. The DLM showed delta wave and QT interval were important to identify the PSVT.

Conclusion

The proposed DLM demonstrated a high performance in identifying PSVT during normal sinus rhythm. Thus, it can be used as a rapid, inexpensive, point-of-care means of identifying PSVT in patients.
Infant Mortality and the Repeal of Federal Prohibition
Authors: Jacks David S , Pendakur Krishna , Shigeoka Hitoshi
Published: February 2021

Using new data on county-level variation in alcohol prohibition from 1933 to 1939, we investigate whether the repeal of federal prohibition increased infant mortality, both in counties and states that repealed and in neighbouring counties. We find that repeal is associated with a 4.0% increase in infant mortality rates in counties that chose wet status via local option elections or state-wide legislation and with a 4.7% increase in neighbouring dry counties, suggesting a large role for cross-border policy externalities. These estimates imply that roughly twenty-seven thousand excess infant deaths could be attributed to the repeal of federal prohibition in this period.
The novel cystatin C, lactate, interleukin-6, and N-terminal pro-B-type natriuretic peptide (CLIP)-based mortality risk score in cardiogenic shock after acute myocardial infarction
Authors: Ceglarek Uta , Schellong Paul , Rosolowski Maciej , Scholz Markus , Willenberg Anja , Kratzsch Jürgen , Zeymer Uwe , Fuernau Georg , de Waha-Thiele Suzanne , Büttner Petra , Jobs Alexander , Freund Anne , Desch Steffen , Feistritzer Hans-Josef , Isermann Berend , Thiery Joachim , Pöss Janine , Thiele Holger
Published: February 2021

Background 

Cardiogenic shock (CS) complicating acute myocardial infarction (AMI) still reaches excessively high mortality rates. This analysis is aimed to develop a new easily applicable biomarker-based risk score.

Methods and results 

A biomarker-based risk score for 30-day mortality was developed from 458 patients with CS complicating AMI included in the randomized CULPRIT-SHOCK trial. The selection of relevant predictors and the coefficient estimation for the prognostic model were performed by a penalized multivariate logistic regression analysis. Validation was performed internally, internally externally as well as externally in 163 patients with CS included in the randomized IABP-SHOCK II trial. Blood samples were obtained at randomization. The two trials are registered with ClinicalTrials.gov (NCT01927549 and NCT00491036), are closed to new participants, and follow-up is completed. Out of 58 candidate variables, the four strongest predictors for 30-day mortality were included in the CLIP score (cystatin C, lactate, interleukin-6, and N-terminal pro-B-type natriuretic peptide). The score was well calibrated and yielded high c-statistics of 0.82 [95% confidence interval (CI) 0.78–0.86] in internal validation, 0.82 (95% CI 0.75–0.89) in internal-external (temporal) validation, and 0.73 (95% CI 0.65–0.81) in external validation. Notably, it outperformed the Simplified Acute Physiology Score II and IABP-SHOCK II risk score in prognostication (0.83 vs 0.62; P < 0.001 and 0.83 vs. 0.76; P = 0.03, respectively).

Conclusions 

A biomarker-only score for 30-day mortality risk stratification in infarct-related CS was developed, extensively validated and calibrated in a prospective cohort of contemporary patients with CS after AMI. The CLIP score outperformed other clinical scores and may be useful as an early decision tool in CS.
Insights into the structure and role of seed-borne bacteriome during maize germination
Authors: Figueiredo dos Santos Lidiane , Fernandes Souta Julie , de Paula Soares Cleiton , Oliveira da Rocha Letícia , Luiza Carvalho Santos Maria , Grativol Clicia , Fernando Wurdig Roesch Luiz , Lopes Olivares Fabio
Published: February 2021

Seed germination events modulate microbial community composition, which ultimately influences seed-to-seedling growth performance. Here, we evaluate the germinated maize (variety SHS 5050) root bacterial community of disinfected seed (DS) and non-disinfected seed (NDS). Using a gnotobiotic system, sodium hypochlorite (1.25%; 30 min)-treated seeds showed a reduction of bacterial population size and an apparent increase of bacterial community diversity associated with a significant selective reduction of Burkholderia-related sequences. The shift in the bacterial community composition in DS negatively affects germination speed, seedling growth and reserve mobilization rates compared with NDS. A synthetic bacterial community (syncom) formed by 12 isolates (9 Burkholderia spp., 2 Bacillus spp., and 1 Staphylococcus sp.) obtained from natural microbiota maize seeds herein was capable of recovering germination and seedling growth when reintroduced in DS. Overall, results showed that changes in bacterial community composition and selective reduction of Burkholderia-related members' dominance interfere with germination events and the initial growth of the maize. By cultivation-dependent and -independent approaches, we deciphered seed–maize microbiome structure, bacterial niches location and bacterial taxa with relevant roles in seedling growth performance. A causal relationship between seed microbial community succession and germination performance opens opportunities in seed technologies to build-up microbial communities to boost plant growth and health.
A Chromosome-Painting-Based Pipeline to Infer Local Ancestry under Limited Source Availability
Authors: Molinaro Ludovica , Marnetto Davide , Mondal Mayukh , Ongaro Linda , Yelmen Burak , Lawson Daniel John , Montinaro Francesco , Pagani Luca
Published: February 2021

Contemporary individuals are the combination of genetic fragments inherited from ancestors belonging to multiple populations, as the result of migration and admixture. Isolating and characterizing these layers are crucial to the understanding of the genetic history of a given population. Ancestry deconvolution approaches make use of a large amount of source individuals, therefore constraining the performance of Local Ancestry Inferences when only few genomes are available from a given population. Here we present WINC, a local ancestry framework derived from the combination of ChromoPainter and NNLS approaches, as a method to retrieve local genetic assignments when only a few reference individuals are available. The framework is aided by a score assignment based on source differentiation to maximize the amount of sequences retrieved and is capable of retrieving accurate ancestry assignments when only two individuals for source populations are used.
Development and Initial Validation of the Adolescents’ Ageism Toward Older Adults Scale
Authors: Marchetti Anna , Lommi Marzia , Barbaranelli Claudio , Piredda Michela , De Marinis Maria Grazia , Matarese Maria
Published: February 2021

Background and Objectives

Ageism toward older adults, prevalent in contemporary societies, seems to be internalized during childhood and consolidated during adolescence. Although several instruments have been developed to measure adolescents’ ageism, they present a number of limitations. The study aimed at developing a new instrument, the Adolescents’ Ageism Toward Older Adults Scale, and testing its psychometric properties.

Research Design and Methods

A three-phase process was followed: The instrument’s items were developed empirically from focus groups of adolescents; its content validity was evaluated; and finally, its psychometric properties were tested through a multicenter cross-sectional study involving 575 adolescents.

Results

Exploratory factor analysis of the new scale retained 33 items grouped into 6 factors: moodiness, traditionalism, physical deterioration, antitechnologism, sageness, and sociability. Confirmatory factor analysis evidenced a second-order factor structure. Omega coefficients measuring internal consistency were above the cutoff of 0.60 for the 6 factors except for antitechnologism. Intraclass correlation coefficients for the entire scale and for the 6 factors were greater than 0.70, again excepting antitechnologism.

Discussion and Implications

The Adolescents’ Ageism Toward Older Adults Scale is a promising measure of adolescents’ negative and positive attitudes toward older adults. Its validation highlighted some criticalities that can be resolved by a few modifications. Further testing of the scale should be conducted after these modifications.
Students’ experience and adherence to containment measures during COVID-19 in Switzerland
Authors: Zysset Annina E , Schlatter Nadine , von Wyl Agnes , Huber Marion , Volken Thomas , Dratva Julia
Published: February 2021

Background

Young adults are not considered a risk group, but the public health response to COVID-19 impacts all citizens. We investigated the impact on young adults’ and their adherence to containment measures addressing potential gender differences.

Methods

In April 2020 12 341 students of the Zurich University of Applied Sciences were invited to a longitudinal health survey. Survey topics spanned socio-demographic data, students’ health status and behavior, COVID-19 specific impact, concerns, information sources, adherence to containment measures, and trust in government bodies. Group comparisons by gender and multivariate ordinal regression models assessing adherence to restrictions of mobility and social contacts were conducted (n = 2373).

Results

Mean age was 26.4 (SD = 5.6), 70% were female. 43.5% reported some concern about their own health, 2.7% stated major worries. Women experienced more conflicts (p < 0.000) and, enjoyed time with the family more (p < 0.000). Men felt less locked up (p = 0.001). The most frequented COVID-19 information source was public media (48%) and confidence in government bodies was high (82%) for both genders. Men yielded lower adjusted odds (OR; 95%-CI) of adherence regarding the following measures: social distancing (0.68; 0.53–0.87), non-utilization of public transport (0.74; 0.56–0.97), 5-person limit for social gatherings (0.47; 0.35–0.64) and the stay at home rule (0.64; 0.51–0.82).

Conclusion

Early in the pandemic a high degree of adherence was observed in this young academic population. Containment measures restricting movement and social contact yielded considerable differences by gender, information source and perceived susceptibility to the virus. More targeted communication may increase adherence regarding mobility restrictions.
Validation of the Personal Social Capital Scale-16 in Portugal: preliminary data on Portuguese and immigrants
Authors: Nascimento Paulo , Roberto Magda Sofia , Santos Ana Sofia
Published: February 2021

Objectives

The Personal Social Capital Scale 16 (PSCS-16) is a self-report measure used to assess social capital, as a social determinant of health. To guarantee validated measures of this construct, the psychometric properties of the Portuguese version of the PSCS-16 were studied.

Methods

The PSCS-16 comprises 16 items, organized in two scales: bonding and bridging social capital. A convenience sample of 280 participants was collected through an online survey. For construct validity, we used confirmatory factor analysis, and convergent and discriminant validity through the average variance extracted (AVE) and correlations. For reliability, we used: The Spearman–Brown split-half and the omega hierarchical coefficient. Correlations were made between the PSCS-16 and socio-demographic variables.

Results

A first-order model depicting two oblique factors was supported, suggesting the use of the two scales. Evidence of convergent validity was achieved: acceptable AVE and associations between social capital and emotional self-disclosure. For discriminant validity, the AVE values surpassed the squared correlation between bonding and bridging, and associations with sexual health were found to be absent. Reliability was good. Additional correlations: A positive association between the education level and bridging social capital and participants with an immigrant status having more bridging social capital.

Conclusions

Preliminary findings support the Portuguese version of PSCS-16 as suitable to evaluate social capital. Contributions are highlighted: the need to study correlates of social capital, particularly crossing migrations, social capital and mental health; and confirming the structure found by measuring its invariance.
‘Alcohol causes cancer’: a difficult message for Australians to swallow
Authors: May Natalie Jane , Eliott Jaklin , Crabb Shona
Published: February 2021

Alcohol is a modifiable risk factor for cancer. Public awareness of the link between alcohol and cancer risk is poor; thus, alcohol consumers may be unknowingly putting themselves at increased risk of cancer. One way to raise awareness of alcohol-related cancer is through placing labels warning of cancer risk on alcoholic beverage containers; however, little is known about the impact of such labels. We conducted seven focus groups, comprising participants who self-identified as low-to-moderate alcohol consumers, to gauge public attitudes towards the labels and messages relating to alcohol-related cancer risk. Transcripts of discussions were coded to identify emergent themes. Participants expressed a negative response to the alcohol warning labels, and their talk worked to challenge the legitimacy of alcohol-related cancer messages, and the entities responsible for disseminating the information. These responses functioned to counter any implied recommendation for reduction in speakers’ alcohol consumption. These findings illustrate how the general population make sense of information about health risks, using this knowledge to make decisions about personal behaviour. In combination with other public health initiatives, alcohol-warning labels have the potential to increase awareness of cancer risk and help in the fight against cancer, but any messaging will need to account for probable consumer resistance.
Double Hurwitz Numbers and Multisingularity Loci in Genus 0
Authors: Kazarian Maxim , Lando Sergey , Zvonkine Dimitri
Published: February 2021

In the Hurwitz space of rational functions on ${{\mathbb{C}}}\textrm{P}^1$ with poles of given orders, we study the loci of multisingularities, that is, the loci of functions with a given ramification profile over 0. We prove a recursion relation on the Poincaré dual cohomology classes of these loci and deduce a differential equation on Hurwitz numbers.
Potential applications of halophilic microorganisms for biological treatment of industrial process brines contaminated with aromatics
Authors: Mainka Thomas , Weirathmüller David , Herwig Christoph , Pflügl Stefan
Published: February 2021

Saline wastewater contaminated with aromatic compounds can be frequently found in various industrial sectors. Those compounds need to be degraded before reuse of wastewater in other process steps or release to the environment. Halophiles have been reported to efficiently degrade aromatics, but their application to treat industrial wastewater is rare. Halophilic processes for industrial wastewater treatment need to satisfy certain requirements: a continuous process mode, low operational expenditures, suitable reactor systems and a monitoring and control strategy. The aim of this review is to provide an overview of halophilic microorganisms, principles of aromatic biodegradation, and sources of saline wastewater containing aromatics and other contaminants. Finally, process examples for halophilic wastewater treatment and potential process monitoring strategies are discussed. To further illustrate the significant potential of halophiles for saline wastewater treatment and to facilitate development of ready-to-implement processes, future research should focus on scale-up and innovative process monitoring and control strategies.
Effect of Terbufos (Organophosphate) on the Cadaveric Colonization Process: Implications for Postmortem Interval Calculation
Authors: Jales J T , Barbosa T M , Soares V P , Gama R A
Published: February 2021

The determination of necrophagous fly specie’s development time is considered an accurate method for estimating postmortem interval (PMI). However, pesticides and other chemicals can alter the flies’ life cycle, inducing errors in PMI estimation. Thus, this work aimed to evaluate the effect of different doses of Terbufos (Organophosphates) on the temporal dispersion pattern and development of immature dipterans associated with decaying rat carcasses. For this, 150 g female Wistar rats received, via gavage, 200 µl of Terbufos (5 or 10 mg/kg) or distilled water (control) and, after 30 min of administration, the animals were euthanized and distributed in suspended traps to decompose under environmental conditions. The dispersing immatures were collected daily, and their development time was monitored until the emergence of adult flies. After data analysis, it was observed that Terbufos altered 1) the temporal pattern of larval dispersion; 2) the composition and structure of the colonizing assemblage (emerged adults); 3) species’ development time, accelerating or delaying their cycle, depending on the dose used; and 4) the califorids and sarcophagids emergence rate, increasing the mortality of pupae from intoxicated carcasses. Thus, this work demonstrates experimentally that Terbufos directly influences the development of flies with forensic potential and discusses the implications for PMI estimation, which can assist in future investigative processes with suspected poisoning by this organophosphate.
Making Masters Moral: Household Subordinates and Upward Social Discipline in Late Medieval Basel
Authors: Grace Philip
Published: February 2021

This article examines how late medieval non-nuclear household relationships shaped the pursuit of honorable social status. It examines in detail several witness depositions from the Basel municipal court (Schultheissengericht) in the late fifteenth and early sixteenth century. Historians have long noted the concern of householders to regulate the morality of their servants and apprentices. However, the study will demonstrate that subordinates could and did defend their own household honor by policing the morality of their masters and other household members, both through verbal shaming and through the distinctive strategic option of refusing to work. The importance of subordinates as arbiters of honorable status was recognized and exploited by individual masters, guilds, and courts; in the region around Basel, it could even be formalized in the written genre of a Schelmbrief (shame-document). Social discipline was not simply a question of householder-masters controlling their subordinates, but also the reverse.
Golgi-localized membrane protein AtTMN1/EMP12 functions in the deposition of rhamnogalacturonan II and I for cell growth in Arabidopsis
Authors: Hiroguchi Akihiko , Sakamoto Shingo , Mitsuda Nobutaka , Miwa Kyoko
Published: February 2021

Appropriate pectin deposition in cell walls is important for cell growth in plants. Rhamnogalacturonan II (RG-II) is a portion of pectic polysaccharides; its borate crosslinking is essential for maintenance of pectic networks. However, the overall process of RG-II synthesis is not fully understood. To identify a novel factor for RG-II deposition or dimerization in cell walls, we screened Arabidopsis mutants with altered boron (B)-dependent growth. The mutants exhibited alleviated disorders of primary root and stem elongation, and fertility under low B, but reduced primary root lengths under sufficient B conditions. Altered primary root elongation was associated with cell elongation changes caused by loss of function in AtTMN1 (Transmembrane Nine 1)/EMP12, which encodes a Golgi-localized membrane protein of unknown function that is conserved among eukaryotes. Mutant leaf and root dry weights were lower than those of wild-type plants, regardless of B conditions. In cell walls, AtTMN1 mutations reduced concentrations of B, RG-II specific 2-keto-3-deoxy monosaccharides, and rhamnose largely derived from rhamnogalacturonan I (RG-I), suggesting reduced RG-II and RG-I. Together, our findings demonstrate that AtTMN1 is required for the deposition of RG-II and RG-I for cell growth and suggest that pectin modulates plant growth under low B conditions.
Pneumocystis jirovecii among patients with cystic fibrosis and their household members
Authors: Morilla Ruben , Medrano Francisco J , Calzada Ana , Quintana Esther , Campano Elena , Friaza Vicente , Calderón Enrique J , de la Horra Carmen
Published: February 2021

We conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10 patients with CF and 15 household members were collected at baseline and 2 weeks later. P. aeruginosa and S. pneumoniae were assessed using standardized culture methods and PCR, and P. jirovecii was assessed using real and nested PCR, genotyping the positive samples by direct sequencing. P. aeruginosa cultures were positive for 7/10 (70%) of patients with CF at baseline and was identified by PCR in 8/10 (80%) of cases at baseline and 2 weeks later. S. pneumoniae cultures were negative for all patients, but the microorganism was identified by PCR in two cases. P. jirovecii was detected by real time and nested PCR in 5/10 (50%) of the patients at the two time points. In the household members, P. aeruginosa and P. jirovecii were identified in 7/15 (46.7%), and S. pneumoniae was identified in 8/15 (53,3%). The concordance of positive or negative pairs of patients with CF and their household members was 33.3% (5/15) for P. aeruginosa, 46.7% (7/15) for S. pneumonia and 93.3% (14/15) for P. jirovecii. The concordance for P. jirovecii genotypes among five pairs with available genotype was 100%. This study suggests for the first time the possible transmission of Pneumocystis in the home of patients with CF, indicating that patients and their household members are reservoirs and possible sources of infection.

Lay Summary

This study suggests for the first time the possible transmission of Pneumocystis in the family environment of patients with cystic fibrosis, indicating that patients and their household members are reservoirs and possible sources of this infection.
Evidence of chromospheric molecular hydrogen emission in a solar flare observed by the IRIS satellite
Authors: Mulay Sargam M , Fletcher Lyndsay
Published: February 2021

We have carried out the first comprehensive investigation of enhanced line emission from molecular hydrogen, H2 at 1333.79 Å, observed at flare ribbons in SOL2014-04-18T13:03. The cool H2 emission is known to be fluorescently excited by Si iv 1402.77 Å UV radiation and provides a unique view of the temperature minimum region (TMR). Strong H2 emission was observed when the Si iv 1402.77 Å emission was bright during the flare impulsive phase and gradual decay phase, but it dimmed during the GOES peak. H2 line broadening showed non-thermal speeds in the range 7–18 $\rm {km\,s}^{-1}$, possibly corresponding to turbulent plasma flows. Small red (blue) shifts, up to 1.8 (4.9) $\rm {km\,s}^{-1}$ were measured. The intensity ratio of Si iv 1393.76 Å and Si iv 1402.77 Å confirmed that plasma was optically thin to Si iv (where the ratio = 2) during the impulsive phase of the flare in locations where strong H2 emission was observed. In contrast, the ratio differs from optically thin value of 2 in parts of ribbons, indicating a role for opacity effects. A strong spatial and temporal correlation between H2 and Si iv emission was evident supporting the notion that fluorescent excitation is responsible.
Gaussian process modelling for improved resolution in Faraday depth reconstruction
Authors: Ndiritu S W , Scaife A M M , Tabb D L , Cárcamo M , Hanson J
Published: February 2021

The incomplete sampling of data in complex polarization measurements from radio telescopes negatively affects both the rotation measure (RM) transfer function and the Faraday depth spectra derived from these data. Such gaps in polarization data are mostly caused by flagging of radio frequency interference and their effects worsen as the percentage of missing data increases. In this paper we present a novel method for inferring missing polarization data based on Gaussian processes (GPs). GPs are stochastic processes that enable us to encode prior knowledge in our models. They also provide a comprehensive way of incorporating and quantifying uncertainties in regression modelling. In addition to providing non-parametric model estimates for missing values, we also demonstrate that GP modelling can be used for recovering rotation measure values directly from complex polarization data, and that inferring missing polarization data using this probabilistic method improves the resolution of reconstructed Faraday depth spectra.
First light from tidal disruption events
Authors: Bonnerot Clément , Lu Wenbin , Hopkins Philip F
Published: February 2021

When a star comes too close to a supermassive black hole, it gets torn apart by strong tidal forces in a tidal disruption event, or TDE. Half of the elongated stream of debris comes back to the stellar pericentre where relativistic apsidal precession induces a self-crossing shock. As a result, the gas gets launched into an outflow that can experience additional interactions, leading to the formation of an accretion disc. We carry out the first radiation–hydrodynamics simulations of this process, making use of the same injection procedure to treat the self-crossing shock as in our previous adiabatic study. Two sets of realistic parameters of the problem are considered that correspond to different strengths of this initial interaction. In both cases, we find that the injected matter has its trajectories promptly circularized by secondary shocks taking place near the black hole. However, the generated internal energy efficiently diffuses away in the form of radiation, which results in a thin vertical profile of the formed disc. The diffusing photons promptly irradiate the surrounding debris until they emerge with a bolometric luminosity of $L\approx 10^{44} \, \rm erg\, s^{-1}$. Towards the self-crossing shock, diffusion is, however, slowed that results in a shallower luminosity increase, with a potentially significant component in the optical band. Matter launched to large distances continuously gains energy through radiation pressure, which can cause a significant fraction to become unbound. This work provides direct insight into the origin of the early emission from TDEs, which is accessed by a rapidly increasing number of observations.
The tune of the Universe: the role of plasma in tests of strong-field gravity
Authors: Cardoso Vitor , Guo Wen-Di , Macedo Caio F B , Pani Paolo
Published: February 2021

Gravitational-wave (GW) astronomy, together with precise pulsar timing and long baseline interferometry, is changing our ability to perform tests of fundamental physics with astrophysical observations. Some of these tests are based on electromagnetic (EM) probes or electrically charged bodies, and assume an empty Universe. However, the cosmos is filled with plasma, a dilute medium which prevents the propagation of low-frequency, small-amplitude EM waves. We show that the plasma hinders our ability to perform some strong-field gravity tests, in particular: (i) nonlinear plasma effects dramatically quench plasma-driven super-radiant instabilities; (ii) the contribution of EM emission to the inspiral of charged black-hole binaries is strongly suppressed; (iii) EM-driven secondary modes, although present in the spectrum of charged black holes, are excited to negligible amplitude in the GW ringdown signal. The last two effects are relevant also in the case of massive fields that propagate in vacuum and can jeopardize tests of modified theories of gravity containing massive degrees of freedom.
GraphBind: protein structural context embedded rules learned by hierarchical graph neural networks for recognizing nucleic-acid-binding residues
Authors: Xia Ying , Xia Chun-Qiu , Pan Xiaoyong , Shen Hong-Bin
Published: February 2021

Knowledge of the interactions between proteins and nucleic acids is the basis of understanding various biological activities and designing new drugs. How to accurately identify the nucleic-acid-binding residues remains a challenging task. In this paper, we propose an accurate predictor, GraphBind, for identifying nucleic-acid-binding residues on proteins based on an end-to-end graph neural network. Considering that binding sites often behave in highly conservative patterns on local tertiary structures, we first construct graphs based on the structural contexts of target residues and their spatial neighborhood. Then, hierarchical graph neural networks (HGNNs) are used to embed the latent local patterns of structural and bio-physicochemical characteristics for binding residue recognition. We comprehensively evaluate GraphBind on DNA/RNA benchmark datasets. The results demonstrate the superior performance of GraphBind than state-of-the-art methods. Moreover, GraphBind is extended to other ligand-binding residue prediction to verify its generalization capability. Web server of GraphBind is freely available at http://www.csbio.sjtu.edu.cn/bioinf/GraphBind/.
Adverse Childhood Experiences and Traumatic Stress Disorders
Authors: Grasso Damion J.
Published: February 2021

Adverse childhood experiences (ACEs) refer to a broad range of events that can reflect physical or psychological threats to safety, as well as deprivation of basic needs, essential resources, or caregiving necessary for children to thrive and attain healthy development. ACEs can constitute potentially traumatic experiences or nontraumatic adversities with the potential to exacerbate trauma-related impairment or compromise trauma recovery and resilience. This chapter explores ACEs in the context of trauma and trauma-related impairment across the life span. It covers research employing variable- and person-centered analytic strategies for quantifying cumulative and unique constellations of ACEs that probabilistically co-occur, contemporaneously or across development, to influence risk and resilience. Studies examining intergenerational patterns and biological correlates are introduced and progress toward delineating causal risk mechanisms discussed. In addition, several existing tools and methods for assessing ACEs in young and school-age children, adolescents, and adults are summarized. Clinical and public health implications of ACE screening in healthcare, schools, and other settings are considered, as is the clinical application of research on ACEs in trauma-specific prevention and treatment. The chapter concludes with a focus on future research priorities.
The Invention of Terrorism in Nineteenth-Century Europe, Russia, and the United States
Authors: Dietze Carola
Published: February 2021

This chapter examines the emergence of terrorism. It argues that five men invented terrorist tactics in a transnational learning process between 1858 and 1866 in Europe, the United States, and Russia. After a systematic reflection on terrorism’s sociopolitical logic and its preconditions, the chapter analyzes Felice Orsini’s attempt to assassinate Napoleon III and the media reactions. This case is interpreted as the beginning of the invention of terrorism. News of Orsini’s deed traveled to America and had inspired John Brown, who changed his tactics from guerilla war to terrorism when he planned his raid on Harpers Ferry. Oskar Wilhelm Becker, John Wilkes Booth, and Dmitrii Vladimirovich Karakozov were the three most significant imitators of both Orsini’s and Brown’s deeds. They finalized the tactic by universalizing it politically and developing the claim of responsibility (Bekennerschreiben). With these developments the terrorist tactic as we know it today was fully developed.
A Mobile Phone in One Hand and Erskine May in the Other: The European Research Group’s Parliamentary Revolution
Authors: Murray C R G , Armstrong Megan A
Published: February 2021

It has become axiomatic that backbench Members of Parliament (MPs) at Westminster have limited the capacity for independent action under the burdens of constituency business and whipped votes. Even the limited avenues available for such MPs to shine, such as select committees, are often illusory because parliamentarians have little time to prepare the materials or brief themselves on any but the highest profile witnesses. The political parties have benefitted from this state of affairs; docile MPs make for reliable votes. The rise of the European Research Group (ERG) as a parliamentary force disrupts this narrative. Galvanised by single-issue opposition to the UK’s involvement in ‘Europe’, encompassing both the European Union (EU) and the European Convention on Human Rights (ECHR), the Group successfully exploited the balance of power in the Commons during the 2017–2019 Parliamentary Session. This article analyses the methods by which the Group’s members magnified their influence over Brexit debates.
Loss, grief and healing: accompaniment in time of COVID-19
Authors: Corpuz Jeff Clyde G
Published: February 2021

This paper is a response to the recent correspondence published in this journal where the author rightly stated that need to redirect one’s perception on the different realities of life such as death. This paper discusses the difficulties associated with loss, grief and healing in the time of COVID-19 pandemic. This paper further adds the importance of accompaniment to people experiencing the complicated grief process. The COVID-19 pandemic is affecting the way one overcome grief where the bereaved are required to grieve without the support of usual social, cultural and religious rituals.
Local R&D support as a driver of network diversification: A cross-regional comparison in Japan
Authors: Takano Keisuke , Okamuro Hiroyuki
Published: February 2021

This study compares the effects of local research and development (R&D) support programs on firm performance in three neighboring prefectures in the same district in Japan. Particularly, we focus on the regional and industrial diversification of transaction networks as the outcome to evaluate regional innovation policies for higher competitiveness. One of these prefectures has a large industrial agglomeration around world-leading manufacturers, which is not applicable to the other two prefectures. Empirical evaluation based on a firm-level dataset confirms only weak and partial effects of local R&D support on business diversification, but provides evidence of different consequences of local innovation policies, reflecting the differences in industrial structure, geographical positions, and policy schemes.
Of Traits and Trees: Probabilistic Distances under Continuous Trait Models for Dissecting the Interplay among Phylogeny, Model, and Data
Authors: Adams Richard H , Blackmon Heath , DeGiorgio Michael
Published: February 2021

Stochastic models of character trait evolution have become a cornerstone of evolutionary biology in an array of contexts. While probabilistic models have been used extensively for statistical inference, they have largely been ignored for the purpose of measuring distances between phylogeny-aware models. Recent contributions to the problem of phylogenetic distance computation have highlighted the importance of explicitly considering evolutionary model parameters and their impacts on molecular sequence data when quantifying dissimilarity between trees. By comparing two phylogenies in terms of their induced probability distributions that are functions of many model parameters, these distances can be more informative than traditional approaches that rely strictly on differences in topology or branch lengths alone. Currently, however, these approaches are designed for comparing models of nucleotide substitution and gene tree distributions, and thus, are unable to address other classes of traits and associated models that may be of interest to evolutionary biologists. Here, we expand the principles of probabilistic phylogenetic distances to compute tree distances under models of continuous trait evolution along a phylogeny. By explicitly considering both the degree of relatedness among species and the evolutionary processes that collectively give rise to character traits, these distances provide a foundation for comparing models and their predictions, and for quantifying the impacts of assuming one phylogenetic background over another while studying the evolution of a particular trait. We demonstrate the properties of these approaches using theory, simulations, and several empirical data sets that highlight potential uses of probabilistic distances in many scenarios. We also introduce an open-source R package named PRDATR for easy application by the scientific community for computing phylogenetic distances under models of character trait evolution.[Brownian motion; comparative methods; phylogeny; quantitative traits.]
Observing Plasticity of the Auditory System: Volumetric Decreases Along with Increased Functional Connectivity in Aspiring Professional Musicians
Authors: Wenger Elisabeth , Papadaki Eleftheria , Werner André , Kühn Simone , Lindenberger Ulman
Published: February 2021

Playing music relies on several sensory systems and the motor system, and poses strong demands on control processes, hence, offering an excellent model to study how experience can mold brain structure and function. Although most studies on neural correlates of music expertise rely on cross-sectional comparisons, here we compared within-person changes over time in aspiring professionals intensely preparing for an entrance exam at a University of the Arts to skilled amateur musicians not preparing for a music exam. In the group of aspiring professionals, we observed gray-matter volume decrements in left planum polare, posterior insula, and left inferior frontal orbital gyrus over a period of about 6 months that were absent among the amateur musicians. At the same time, the left planum polare, the largest cluster of structural change, showed increasing functional connectivity with left and right auditory cortex, left precentral gyrus, left supplementary motor cortex, left and right postcentral gyrus, and left cingulate cortex, all regions previously identified to relate to music expertise. In line with the expansion–renormalization pattern of brain plasticity (Wenger et al., 2017a. Expansion and renormalization of human brain structure during skill acquisition. Trends Cogn Sci. 21:930–939.), the aspiring professionals might have been in the selection and refinement period of plastic change.