bluuebunny/crossref_metadata_2025 huggingface dataset:
Counselors’ Perceptions of ADHD Children’s Parent Counseling Processes: A Grounded Theory Approach
Authors:   The Association of Korea Counseling Psychology Education Welfare, OH Ji-Won , SHIN Seo-Won
Published: July 2022

The purpose of this study is to explore counselors’ perceptions regarding the processes ofADHD children’s parent counseling. Interviews from 15 counselors were analyzed using agrounded theory approach. As a result, 8 themes and 19 subcategories were found. Incounseling context, children-centered environments and various pressing issues of parents werereported. Regarding goals and interventions, psycho-educations for a better understanding andacceptance of ADHD, coaching for decreasing hyperactivity, and the provision of empathy andvalidation for emotional stability were reported. Parents’ cognitive, emotional, and behavioralchanges were found as effects. On the other hand, the paucity of social justice interventionsand the lack of time and financial supports were found as limitations. The participants alsosuggested that parent counseling vouchers, self-help groups, and psycho-education programs forteachers and peer parents would help ADHD children and the parents. Implications were alsodiscussed based on these results.
The Repertoire of Saratov Periodicals
Authors: Kasovich A. S.
Published: July 2022

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User-­Oriented Approach to Content Improvement in Official Library Websites
Authors: Gendina N. I. , Kolkova N. I. , Ryabtseva L. N.
Published: July 2022

The creation of official websites of modern libraries has opened up for them fundamentally new opportunities to present a wide range of information resources and services for remote users. The purpose of the article is to reveal the problem of determining the rational composition and placement of headings and subheadings in the content structure of library sites and to present a typical content model as one of the possible means to ensure the orientation of users on the sites of the same type of libraries from the standpoint of a user-oriented approach. A typical model of the content of the official website of the central libraries of the subjects of the Russian Federation is proposed. It can be used in any of the libraries of the types under consideration (republican, regional, autonomous districts and regions, as well as central libraries of cities of federal subordination). The proposed typical model is aimed at overcoming the unreasonable diversity of structural elements by reducing the existing list of headings and subheadings and bringing them to uniformity and to provide mutual understanding between library site developers and remote users. The unification of the content structure of official library websites can become a condition not only for improving the quality of the content of individual sites, but also for ensuring their compatibility to form uniform digital library and information space in Russia.
Professional Competencies of Library Staff Engaged in Information and Analytical Support of Researches
Authors: Peksheva M. A.
Published: July 2022

The analysis of professional competencies of information specialists in domestic libraries operating in the research environment is carried out. The research is based on a questionnaire on the relevance of certain groups of professional competencies: background knowledge and skills, knowledge and skills in the field of scholarly communication, “soft” skills. The target group of the survey included library staff engaged in information and analytical support of fundamental researches of the Russian Academy of Sciences. It is revealed that the overwhelming majority of respondents recognize the relevance of the competencies included in the survey for library practice. Based on the respondents’ responses, competencies are divided into two key categories: universal and specialized. More than 60   % of the competence groups in the sections “background knowledge and skills” and “knowledge and skills in the field of scholarly communication” are classified by the majority of respondents as specialized ones. Almost all “soft” skills are classified by the majority of respondents as universal ones. Competence groups classified as “universal” can be used as the basis for the development of a general competence model for scientific library staff working in a research environment. Competence groups classified as “specialized” – are of importance for the development of variable models of competencies for individual functional roles of employees engaged in information and analytical support of scientific research.
International Cooperation of Library and Information Institutions in the Republic of Uzbekistan
Authors: Tsai I. K.
Published: July 2022

Globalization is closely connected with the process of transition to a common information space. Libraries take an active part in this process. They, thanks to the development of cooperation, can use international experience, evaluate their activities and achievements, identify problems, improve the professional level of library specialists and participate in the development of new concepts for the development of library and information business and culture in general, intensify interaction with state and interstate coordination bodies. The article summarizes the experience of organization of work on the development of international cooperation between the libraries of the Republic of Uzbekistan with libraries and leading information, educational and scientific institutions, publishing houses of foreign countries, diplomatic missions, funds, representative offices of international organizations. It includes international document exchange, readers service within the framework of international interlibrary loan; project activities, preparation and holding of international scientific and practical conferences, symposiums, seminars, exhibitions, etc. The main directions of international activities of the National Library of the Republic of Uzbekistan named after A. Navoi and information resource centers of universities are also described. The author comes to the conclusion that for a fruitful solution of problems, it is necessary to create a legal framework, attract grant funds and organize joint commercial international projects.
Orthogonal Frames in Krein Spaces
Authors: Ferrer Villar Osmin Oberto , Sierra Acosta Arley Yessit , Polo Flórez Osvaldo De Jesus
Published: July 2022

In the present paper we introduce the concept of orthogonal frames in Krein spaces, prove the independence of the choice of the fundamental symmetry, and from this we obtain a number of interesting properties that they satisfy. We show that there is no distinction between orthogonal frames in a Krein space and orthogonal frames in its associated Hilbert. Furthermore, we characterize frames dual to a given frame, which is a useful tool for constructing examples.
Comparative Effect of Cow Dung and Poultry Manure on the Growth of Khaya senegalensis (Desr.) Seedlings
Authors: Oluborode James , Kilasho Adeola , Adebiyi Adewale
Published: July 2022

The study investigated the comparative effect organic fertilizers on the growth of Khaya senegalensis (Desr). Four treatments were used for this research: T1 20g of poultry manure, T2 (20g of cow dung), T3 (10g of poultry Manure + 10g of cow dung), T4 (Control experiment). Growth parameters such as diameter, plant height and number of leaves were measured weekly over a period of twelve (12) weeks. Data obtained were subjected to ANOVA and mean separated using Duncan Multiple Range Test. on the height parameter assessed on Khaya Senegalensis, the highest plant height mean of 8.40cm was recorded in treatment 1(20g of poultry Manure) while the lowest mean plant height 7.19cm was recorded in treatment 4 (Control experiment). Also, the highest mean stem diameter was recorded in Treatment 1 with a value of 0.309mm from while the least was recorded in Treatment 4 with mean value of 2.1175mm. However, highest mean number of leaves was recorded treatment 1 (20g of poultry manure) while the least was observed in T3 (10g of poultry Manure + 10g of cow dung). The results showed that organic manure has considerable effect on the growth of Khaya senegalensis seedlings and it use should be encouraged. T3 (10g of poultry Manure + 10g of cow dung) is hereby recommended to be the best of all in the growth of Khaya senegalensis.
Representative Service Providers and Their Selection in Cloud Computing Domain; A Comprehensive Overview
Authors: Dahal Pranish , Prasai Ritika
Published: July 2022

The popularity of cloud computing is growing owing to its large data storage capacity and high computation power. It provides online, on-demand, scalable application solution, removes hardware and software barriers for non-specialist, rapidly integrates and deploys desired and necessary facilities, supports quick upgrading and addition of features. Users get benefitted with the selection of the appropriate cloud computing platform for their projects. Here, our paper provides a comprehensive overview of the services provided to the users by the most common cloud computing service providers. This paper could be used as a reference while selecting the best service provider based on the requirements of the projects.
Evaluasi Pengunjung Terhadap Kualitas Pantai Palippis Sebagai Ruang Publik Tepian Air Berbasis Placemaking
Authors: Firmansyah Firmansyah , Sutomo Slamet Tri , Ali Mukti
Published: July 2022

Palippis Beach is an underwater coastal tourism object (snorkeling) and a rest area, but there is a conflict with managers who prioritize finance and a lack of visitors from year to year, which causes Pantai Palippis being not optimal as a waterfront public space. The aim of this research is to determine the quality of Palippis Beach as a waterfront public space and the recommendations of its development based on the placemaking approach. This research was conducted from November 2019 until February 2020 (4 months). This research used primary and secondary data which collected by field surveys, questionnaires, interviews, documentation and literature studies. The research location takes a part of the rest area which is located between Polewali Mandar and Majene Regencies. The analysis used in this research is Importance Performance Analysis (IPA) method, activity mapping analysis (behavior mapping), and infrastructure analysis. The results showed that the quality value of Palippis Beach using the IPA questionnaire method gets a value of 83.50% which declared as a high quality because it is between 76% -100%. The intensity of use from Palippis Beach scores 54.54% on weekdays and 53.2% on holidays which is considered high because it is in the range of 51% -75%. There are several tourist infrastructure that still need to be equipped, such as tourism services, security, and cleanliness so quality improvement recommendations are required based on the four quadrants resulting from the Cartesian diagram of the IPA method. These directives are recommendations for the development, maintenance, utilization and adjustment of tourist area infrastructure in accordance with applicable standards.
Analisis Penentuan Lokasi Potensial Pengembangan Fasilitas Parkir Pada Kawasan Perdagangan Kecamatan Wajo Kota Makassar
Authors: Rosmiaty Rosmiaty , Akil Arifuddin , Azmy Muhammad Fathien
Published: July 2022

Wajo District was designated as a trade and service center area in the Makassar City Spatial Plan so that it attracts many visitors and has an impact on increasing parking needs. The problem that occurs is the discovery of parking vehicles on road bodies that violate regulations, such as parking on arterial roads, pedestrian paths, and parking at a distance of less than 25 meters from road intersections, causing traffic flow obstruction on several roads. This study aims to determine potential locations and directions for the development of off-street parking facilities in the trading area by maximizing the limited land area. This study aims to determine the location facilities and direction of off-street parking development in the trading area by maximizing the limited land area. The research location is in Wajo District, Makassar City and was carried out in March-November 2020 (9 months). There are two types of data needed in this study, namely secondary data (data on land availability, land function using RTRW, road crossing points, population density, road size, and number of lanes) and primary data (data on the distribution of parking facilities). Determination of the potential parking location is done with the help of a grid-based ArcGis application. From the results of the grid analysis, it was found that 198 grid units were considered very potential and were divided into five distribution groups. The direction for the development of off-street parking facilities is recommended by implementing a vertical rotary parking system, soul parking system, puzzle parking system, tower parking system, and lift parking system and there is a need for firm action such as imposing ticket and fines for vehicle users who violate statutory regulations shrimp related parking.
The Correlation of 4Ps of Marketing in the Business Performance of MSEs in Palayan City, Nueva Ecija
Authors: Jesus Fhrizz S. De , Villanueva Winnie C.
Published: July 2022

The researchers’ primary considerations in conceptualizing what aspects should be introduced into the business marketing strategy with the business performance of Small and Medium Enterprises in Palayan City, Nueva Ecija. This research is critical for companies that want to improve their business performance by providing better service. This study created a business plan for MSEs to use the Marketing Mix and improve customer satisfaction by improving business performance. This study used a quantitative research design and descriptive analysis to describe and summarize the data that was gathered. The research was carried out at 30 licensed MSEs in and around Palayan City, Nueva Ecija. The researchers used a total enumeration technique to simply identify all of the respondents who met the criteria for the study. A total of 30 respondents were surveyed, the majority of whom were rank and file owners of licensed SMEs. The information gathered from the respondents was encoded, tallied, and analyzed using statistical tools such as Percentage, Frequency Distribution, Weighted Mean, Pearson r, and Thematic Analysis. The findings revealed a significant relationship between the respondents' marketing mix and business performance. Furthermore, the majority of respondents are dissatisfied with the business performance they are providing. The MSE owner can adapt improvements in business performance such as promotional strategy, lowering expenses, improving product quality, and providing good service to increase sales revenue and business growth. It is recommended that the researchers' development plan be used to assist respondents in gaining more knowledge about improving business performance to increase efficiency and effectiveness and may serve as a guide for the MSE to approach, which would increase the business's productivity and sale revenue in the long run.
A Narrative Study of Professional Growth of Outstanding Elementary School Music Teachers
Authors: Jiaxin Li
Published: July 2022

Outstanding elementary school music teachers are outstanding representatives of the elementary school music teacher community, and they are the objects of study and emulation for general elementary school music teachers. Their professional growth experiences can provide inspiration for teacher education institutions to train elementary school music teachers and for general elementary school music teachers to grow professionally on their own. The narrative study of the outstanding elementary school music teachers revealed that the factors affecting their professional growth were from four aspects: personal, family, school and society, and the basic ways of their professional growth were professional learning, teaching and research activities and reflective summaries. It is pointed out that social opinion and encouragement are the key prerequisites for the professional growth of elementary school music teachers, government support and guidance are the important conditions for the professional growth of elementary school music teachers, and school service and management are the basic guarantees for the professional growth of elementary school music teachers, and independent learning and reflection are the inexhaustible motivation for the professional growth of elementary school music teachers.
The (Im)precision of Life Expectancy Numbers
Authors: Hanley James A.
Published: July 2022

Life expectancy figures for countries and population segments are increasingly being reported to more decimal places and used as indicators of the strengths or failings of countries’ health and social systems. Reports seldom quantify their intrinsic statistical imprecision or the age-specific numbers of deaths that determine them.

The SE formulas available to compute imprecision are all model based. This note adds a more intuitive data-based SE method and extends the jackknife to the analysis of event rates more generally. It also describes the relationships between the magnitude of the SE and the numbers of person-years and deaths on which it is based. These relationships can help quantify the statistical noise present in published year-to-year differences in life expectancies, as well as in same-year differences between or within countries.

Agencies and investigators are encouraged to use one of these SEs to report the imprecision of life expectancy numbers and to tailor the number of decimal places accordingly. (Am J Public Health. 2022;112(8):1151–1160. https://doi.org/10.2105/AJPH.2022.306805 )
Automatic Identification System Analysis Package user guide
Authors: Kress Marin , DiJoseph Patricia , Donohue Patrick , Mitchell Kenneth
Published: July 2022

The Automatic Identification System Analysis Package (AISAP) enables acquisition, statistical analysis, and visualization of Automatic Identification System (AIS) data from historic vessel position reports. The web-based AISAP software allows the users to choose which data they want to examine for a specific geographic area, time period, and vessel type(s). Built-in features provide vessel characteristics, arrival and departure information within a geofenced area, vessel travel time between two locations, vessel track line plots, and relative density plots of AIS data reports. AISAP accesses the Nationwide Automatic Identification System database hosted by the United States Coast Guard. This user manual provides training exercises for users to follow to familiarize themselves with AISAP procedures and workflows. These training exercises also provide examples of AISAP products.
Applied Drilling Engineering
Authors: Bourgoyne Adam T. , Millheim Keith K. , Chenevert Martin E. , Young F.S.
Published: July 2022

An industry and academic standard, Applied Drilling Engineering presents engineering science fundamentals as well as examples of engineering applications involving those fundamentals. Two appendices are included, along with numerous examples. Answers are included for every end-of-chapter question.

Solutions to Chapter 8 (http://go.spe.org/ADEsolutions)
Waterflooding
Authors: Willhite G. Paul
Published: July 2022

Waterflooding is an important method of improving recovery, but successful waterflood performance requires a sound design. Waterflooding begins with understanding the basic principles of immiscible displacement, then presents a systematic procedure for designing a waterflood. The emphasis is on fundamental concepts and their application in solving various waterflooding problems. Design procedures that can be prepared as small computer programs and selected computer subprograms for more complex designs are presented.
Theory, Measurement, and Interpretation of Well Logs
Authors: Bassiouni Zaki
Published: July 2022

An indispensable tool, Theory, Measurement and Interpretation of Well Logs introduces the three primary phases of well-logging technology to engineering and geosciences students. This text offers an in-depth study of the electric, radioactive, and acoustic properties of sedimentary rocks. Mathematical and empirical models relate a formation property of interest to the property measured with the logging tool. Openhole logging techniques are covered, along with concepts of traditional and modern tools.
Phase Behavior
Authors: Whitson Curtis H. , Brulé Michael R.
Published: July 2022

This important addition to any petroleum engineer’s library covers all aspects of gas/oil phase behavior and includes a brief discussion of multiphase and vapor/solid phase behavior. Phase Behavior provides the reader with the tools needed to solve problems requiring a description of phase behavior and specific pressure/volume/temperature (PVT) properties. Also included are four appendices, including an overview of understanding laboratory oil PVT reports by M.B. Standing.
Fundamental Principles of Reservoir Engineering
Authors: Towler Brian F.
Published: July 2022

Fundamental Principles of Reservoir Engineering outlines the techniques required for the basic analysis of reservoirs prior to simulation. It reviews rock and fluid properties, reservoir statics, determination of original oil and gas in place by volumetrics and material balances, evaluation of drive mechanisms, fluid flow in porous media, aquifer influx, well testing, fluid distribution and displacement, and decline-curve analysis.
Pressure Transient Testing
Authors: Lee John , Rollins John B. , Spivey John P.
Published: July 2022

Pressure Transient Testing presents the fundamentals of pressure-transient test analysis and design in clear, simple language and explains the theoretical bases of commercial well-test-analysis software. Test-analysis techniques are illustrated with complete and clearly written examples. Additional exercises for classroom or individual practice are provided. With its focus on physical processes and mathematical interpretation, this book appeals to all levels of engineers who want to understand how modern approaches work.
Multilateral Wells
Authors: Hill A.D. , Zhu Ding , Economides Michael J.
Published: July 2022

Multilateral-well technology is revolutionizing the way that reservoirs are accessed by wells. The ability to create wells with multiple branches that can target widely spaced reservoir compartments provides engineers unlimited options in optimizing economic extraction of oil and gas. Along with this opportunity comes the inherent complexity of these well architectures. Multilateral Wells is intended to illuminate the most important aspects of multilateral wells in a concise way and give readers practical tools they can apply in the design and analysis of multilateral wells, while also pointing to the appropriate literature for more advanced studies.
Openhole Log Analysis and Formation Evaluation
Authors: Bateman Richard M.
Published: July 2022

Since the first edition of Openhole Log Analysis and Formation Evaluation , which was published in 1985, the science and art of formation evaluation has seen many innovations. Measurements of formation resistivity have advanced, advances have been made in the design of sonic tools and in the processing of their recorded signals, and new methods of looking at the distribution of pore sizes, their fluid content, and formation flow potential have become widely used. This revised and updated second edition will prepare the reader for a fuller understanding of modern formation evaluation data-gathering methods, as well as illuminate advanced analysis techniques. The book is divided into sections that address a number of formation evaluation themes, including Methods of Gathering Formation Evaluation Data, Methods of Analysis and Application of Results, Openhole Logging Measurements, Analysis of Logs and Cores, and Formation Testing.

Errata ( http://go.spe.org/OPLAerrata )
Robust Networked Multiagent Optimization: Designing Agents to Repair Their Own Utility Functions
Authors: Brown Philip N , Seaton Joshua H , Marden Jason R
Published: July 2022

We study settings in which autonomous agents are designed to optimize a given system-level objective. In typical approaches to this problem, each agent is endowed with a decision-making rule that specifies the agent’s choice as a function of relevant information pertaining to the system’s state. The choices of other agents in the system comprise a key component of this information. This paper considers a scenario in which the designed decisionmaking rules are not implementable in the realized system due to discrepancies between the anticipated and realized information available to the agents. The focus of this paper is to develop methods by which the agents can preserve system-level performance guarantees in these unanticipated scenarios through local and independent redesigns of their own decision-making rules. First, we show a general impossibility result which states that in general settings, there are no local redesign methodologies that can offer any preservation of system-level performance guarantees, even when the affected agents satisfy an inconsequentiality criterion. However, we then show that when system-level objectives are submodular, local redesigns of utility functions do exist which allow nominal performance guarantees to degrade gracefully as information is denied to agents. That is, in these submodular settings, agents can adapt to informational inconsistencies independently without incurring much loss in terms of system-level performance.
On the migrativity properties between uni-nullnorms and overlap (grouping) functions
Authors: Zeng Xiangxiang , Zhu Kuanyun
Published: July 2022

Based on the concepts of the α-migrativity between overlap (grouping) functions and uninorms (nullnorms), in this paper, we introduce and study the α-migrativity between a uni-nullnorm V and a given overlap (grouping) function. First of all, we propose the notion of the (α,O)-migrative uni-nullnorms over a given overlap function O. And then, we explore some equivalent characterizations of the (α,O)-migrativity equation when the uni-nullnorm V pertains to one of the common types. Besides, we consider the concept of (α,G)-migrative uni-nullnorms over a given grouping function G and study the (α,G)-migrativity equation using analogous methods. Moreover, we propose the concept of (α,V)-migrative overlap (grouping) functions over a given uni-nullnorm V and explore the necessary and sufficient conditions of the (α,V)-migrativity equation solutions if the uni-nullnorm V pertains to one certain class.
Gut microbiota profiles in mental disorders and their association with behavior in children and adolescents.
Authors: Soltysova Marcela , Tomova Aleksandra , Ostatnikova Daniela
Published: July 2022

The aim of our work is to summarize the current state of knowledge on gut microbiota changes in children and adolescents with mental illnesses. To our knowledge, this is the first review comparing gut microbiota changes in various mental disorders in children and adolescents.To find the relevant articles, PubMed, Web of Science and Google Scholar databases were searched. Articles in English, presenting original data and comparing the composition of gut microbiota in child psychiatric patients with gut microbiota in healthy children and adolescents were selected. Finally, we identified 42 articles eligible for our purpose. Patients with autism spectrum disorders (ASD) were investigated in majority of them. Smaller number of studies evaluating the gut microbiota in children and adolescents with Attention-deficit hyperactivity disorder (ADHD), Rett syndrome, anorexia nervosa, major depressive disorder and tic disorders were found. Main findings of research are discussed in our review, focusing on association between the gut microbiota and behavioral manifestations. To conclude, gut microbiota in children and adolescent with mental disorders is evidently different from control. Most pronounced changes are seen in children with autism spectrum disorders. These changes might be associated with often present gastrointestinal manifestations but, in general, also play a pathogenetic role in behavioral disorders. Based on research findings we assume, that there is a perspective of use of gut microbiota correction in order to improve clinical symptoms in psychiatry.
WITHDRAWN: Antibody responses 8-20 weeks after two doses of SARS-CoV-2 vaccine (CoronaVac) in hemodialysis patients: Results of a single center in northeast Turkey
Authors: Erdoğdu Halil İbrahim , Atalay Eray , Kahraman İhsan , Keleşoğlu Royça , Karakaya Tuğba , Ejder Serkan
Published: July 2022

Background: The study aimed to determine the antibody responses of hemodialysis (HD) patients receiving the second dose of inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine (CoronaVac).Methods: Patients were divided into two groups according to the time elapsed since the second dose of SARS-CoV-2 vaccine: group 1 was 14-20 weeks after second dose of vaccine, and group 2 was 8-13 weeks. In addition, patients were divided according to their antibody (anti-spike IgG) response as inadequate < 0.2 µg /mL and adequate response ≥ 0.2 µg /mL. Factors such as age, dialysis vintage, diabetes mellitus and Kt/V which may affect response to SARS-CoV-2 vaccine were evaluated.Results: Out of 67 patients, according to exclusion criteria 30 patients were excluded. 52.7% and 33.3%patients had adequate response, in those groups, respectively. In the group 2, the age of those who did not have an antibody response was 65.0±10.4, while those with a response were 50.5±11.0 years old (p=0.015, 95% CI).In addition, one of our two patients in the group 1, who received four doses of 40 µgr recombinant hepatitis B vaccine and did not get anti-HBs antibody response (anti-HBs <10 IU/mL) was also unresponsive to the second dose of SARS-CoV-2 vaccine. Antibody responses to the SARS-CoV-2 vaccine in both groups were not different in terms of the compared characteristics of the patients (p>0.050).Conclusion: The second dose of SARS-CoV-2 vaccine responses in HD patients was analyzed and the positive effect of being relatively younger was determined 8-13 weeks after second dose.
Autonomous Electrochemical System for Ammonia Oxidation Reaction Measurements at the International Space Station
Authors: Cabrera Carlos , Morales-Navas Camila , Martinez Roberto Rodriguez , Vidal-Iglesias Francisco , Peña-Duarte Armando , Soto-Pérez Joesene , Trinidad-Pérez Pedro , Solla-Gullón José , Tzvetkov Toshko , Smotkin Eugene , Nicolau Eduardo , Feliu Juan , Doan Jonathan
Published: July 2022

An autonomous electrochemical system prototype for ammonia oxidation reaction (AOR) measurements was efficiently done inside a 4" x 4" x 8" 2U Nanoracks module at the International Space Station (ISS). This system, the Ammonia Electrooxidation Lab at the ISS (AELISS), included an autonomous electrochemical system that complied with NASA ISS nondisclosure agreements, power, safety, security, size constrain, and material compatibility established for space missions. The integrated autonomous electrochemical system was tested on-ground and when deployed to the International Space Station as a “proof-of-concept” ammonia oxidation reaction testing space devise. Cyclic voltammetry and chronoamperometry measurements were done at the ISS with a commercially available channel flow-cell with eight screen-printed electrodes, including Ag quasi-reference (AgQRE) and carbon counter electrodes. Pt nanocubes in Carbon Vulcan XC-72R were used as the catalyst for the AOR and 2 μL drop of Pt nanocubes/ Carbon Vulcan XC-72R, 20 wt%, ink was placed on the carbon working electrodes and allowed to dry in air. After the AELISS was prepared for launch to the ISS, a four days deal (two days in the space vehicle Antares and two days transit to the ISS) cause a slight shift on the AgQRE potential. Nevertheless, the AOR cyclic voltametric peak was observed in the ISS and showed ca. 70% current density decrease due to the buoyancy effect in agreement with previous microgravity experiments done at the zero-g airplane.
Breathing pattern, accessory respiratory muscles work, and gas exchange evaluation for prediction of NIV failure in moderate-to-severe COVID-19- associated ARDS after deterioration of respiratory failure outside ICU: the COVID-NIV observational study
Authors: Yaroshetskiy Andrey I. , Merzhoeva Zamira M. , Tsareva Natalia A. , Trushenko Natalia V. , Nuralieva Galia S. , Konanykhin Vasily D. , Krasnoshchekova Anna P. , Avdeev Sergey N.
Published: July 2022

Background: Data on the efficacy of non-invasive ventilation (NIV) after progression of respiratory failure in patients who have already received oxygen therapy, or CPAP outside ICU is limited. The aim of the study was to find predictors of NIV failure based on breathing pattern, gas exchange, and accessory respiratory muscles evaluation in patients who progressed to moderate-to-severe COVID-19 ARDS. Methods: This was a prospective observational study in patients with moderate-to-severe COVID-19-ARDS on NIV (n=80) admitted to COVID-ICU of Sechenov University. Combined success rate for conventional oxygen and CPAP outside ICU was 78.6% (440 of 560 patients). The primary endpoints were intubation rate and mortality. We measured respiratory rate, exhaled tidal volume (Vte), mean peak inspiratory flow (PIF), inspiratory time (Ti), PaO2, SpO2, end-tidal carbon dioxide (PETCO2), and Patrick score, and calculated ROX index, PaO2/FiO2, ventilatory ratio, and alveolar dead space (Vdalv/Vt) on Days 1, 3, 5, 7, 10, and 14. For all significant differences between NIV success and failure groups in measured data we performed ROC-analysis.Results: NIV failure rate in ICU after deterioration of respiratory failure outside ICU was 71.3% (n=57). Patients with the subsequent NIV failure were older at inclusion, more frail, had longer duration of disease before ICU admission, and higher rate of CPAP use outside ICU. ROC-analysis revealed that the following respiratory parameters after 48 hours of NIV can serve as a predictors for NIV failure in moderate-to-severe COVID-19-associated ARDS: PaO2/FiO2 <112 mmHg (AUROC 0.90 (0.93-0.97), p<0.0001); PETCO2 <19.5 mmHg (AUROC 0.84 (0.73-0.94), p<0.0001); VDalv/VT > 0.43 (AUROC 0.78 (0.68-0.90), p<0.0001); ROX-index < 5.02 (AUROC 0.89 (0.81-0.97), p<0.0001); Patrick score > 2 points (AUROC 0.87 (0.78-0.96), p=0.006).Conclusion: In patients who progressed to moderate-to-severe COVID-19-ARDS probability of NIV success rate was about 1/3. Prediction of the NIV failure can be made after 48 hours based on ROX index<5.02, PaO2/FiO2<112 mmHg, PETCO2<19.5 mmHg, and Patrick score >=2.Trial registration: ClinicalTrials.gov identifier: NCT04667923, registered on 16/12/2020. https://clinicaltrials.gov/ct2/show/NCT04667923?term=NCT04667923&draw=2&rank=1
Pirfenidone-loaded Hyaluronic Acid Methacryloyl hydrogel for Preventing Epidural Adhesions after Laminectomy
Authors: ji jiawei , Cheng Jiaqi , Chen Cheng , Lu Yang , Chen Xiaoqing , Zhang Feng
Published: July 2022

Background: It is inevitable that scar formation between the spinal dura and surrounding tissues after laminectomy. While extensive epidural fibrosis, which results in limited nerve root activity and severe pain, is the main cause of postoperative failed-back surgery syndrome.Purpose: Novel biomaterials loading effective drug based on reasonable design are eagerly needed for safe and effective prevention of epidural adhesions.Methods: We filtrated a suitable dose of Pirfenidone (PFD) to load Hyaluronic acid Methacryloyl (HAMA) hydrogel in vitro. And then, we compare PFD-loaded HAMA hydrogel with only using PFD or HAMA hydrogels after laminectomy by in vivo studies.Results: We describe a safe and efficient anti-adhesive PFD-loaded HAMA hydrogel that prevents epidural fibrosis through stable and sustained release of PFD. It was shown that the PFD-loaded HAMA hydrogel effectively inhibited cell penetration and suppressed collagen I/III expression. Thus, it effectively prevented the formation of adhesions through pharmacological and physical processes.Conclusion: The PFD-loaded HAMA hydrogel can effectively prevent adhesion formation in both pharmacological and physical barrier effects.
Case Series: Belimumab in Refractory Lupus Nephritis
Authors: Malaweera Aruni , Dayan Sukhpal , Hoi Alberta , Kitching A Richard , Kent Joanna R
Published: July 2022

In recent trials for the treatment of systemic lupus erythematosus (SLE),
belimumab (a recombinant human IgG1-monoclonal antibody that inhibits B lymphocyte stimulator), in addition to standard immunosuppression, has been shown to improve renal and non‐renal outcomes. We report our experience using belimumab in two cases of refractory lupus nephritis (LN), where renal remission was not achieved using cyclophosphamide, mycophenolate mofetil and other immunosuppressive medications. In one of the cases, belimumab therapy led to a partial remission of LN, improvement in serological markers of SLE and disease activity, which permitted a reduction in prednisolone dosing. Treatment with efficacious therapies early in the course of LN is a desirable therapeutic strategy, to achieve early remission of proteinuria and curtail the development of irreversible chronic renal damage. Further studies are needed to provide information on the effectiveness of belimumab for maintenance of remission, prevention of flares and monitoring for long term complications of B cell modulation.
Mucosal-associated invariant T (MAIT) cell activation and correlation of CD69+ MAIT cell frequency with disease activity in patients with psoriasis
Authors: Qiao Lihui , Ding Yuhong , Liu Shirui , Yan Qi , Chen Yang , Zhang Tong , Guo Xiuying , Pei Jin
Published: July 2022

Mucosa-associated invariant T (MAIT) cells are innate lymphocytes that play a specific role in autoimmune diseases. In particular, the frequency and phenotype of MAIT cells in the pathogenesis of psoriasis is not fully understood. Our study examines the frequency and phenotype of MAIT cells sorted from peripheral blood mononuclear cells (PBMCs) obtained from 53 cases of patients and 44 healthy control.Compared with the healthy control group, the frequency of circulating CD3 + MAIT cells, CD3 + CD8 + MAIT cells, CD4 + CD69 + MAIT and CD8 + CD69 + MAIT cells in psoriasis patients was significantly increased (p < 0.05). While the proportion of CD3 + CD4 + MAIT cells was decreased. Correlations between IL-15 and PASI scores and activated MAIT cells revealed a potential role of MAIT cells in the development of psoriasis. IL-15 was positively correlated with both CD4 + CD69 + MAIT and CD8 + CD69 + MAIT cells. Activated CD3 + MAIT cells and activated CD8 + MAIT cells in patients' serum were both positively correlated with patients' PASI scores, while activated CD4 + MAIT cells and PASI scores were negatively correlated .Our results suggest that the abnormal phenotype of MAIT cells in patients with psoriasis parallels the progression of the disease. These abnormalities may lead to skin and systemic immune disorders in patients with psoriasis.
Bioinspired Spindles using Peptide-DNA Nanotechnology
Authors: Daly Margaret , Nishi Kengo , Klawa Stephen , Hinton Kameryn , Gao Yuan , Freeman Ronit
Published: July 2022

Cytoskeletal assemblies produce functionality through multiscale structural reconfiguration by exploiting a family of protein crosslinkers. Inspired by these principles, we designed an array of complementary peptide-DNA monomers able to form multistranded junctions with different geometries and stabilities and tested their effect on the structural organization of co-polymerized filament-forming peptides. In striking similarity to their biological counterparts, the peptide-DNA crosslinkers organized fibers into bundled architectures with base-pair tunable aspect ratios. Controlling solvent or temperature drove assembly through distinct pathways to form different structural and mechanical states exhibiting multiple levels of hierarchy from the same set of components. The thermal reversibility of DNA crosslinks drove structures to form, disassemble, and rebuild over multiple cycles. Sequential transitioning between bundles and fibers using orthogonal triggers enabled the dosed release of proteins to modulate catalytic reactions. This programmable peptide-DNA nanotechnology framework will pave the way towards the design of hierarchical materials with reconfigurable properties.
Lower abundance of human gut virus species is associated with cancer cachexia
Authors: Stang Axel , Weilert Hauke , Illig Thomas , Schmidt Rudi , Burbach Katharina , Gronauer Raphael , Seifert Martin
Published: July 2022

Cancer cachexia has been linked to gut bacterial alterations, but alterations of gut viruses, mostly bacteriophages, have not yet been explored. We performed shotgun metagenomic sequencing of DNA from stool samples of 78 cachectic and 42 non-cachectic cancer patients. K-mer-based matching to reference databases revealed abundance variations of bacteria and viruses. Beyond bacterial alterations, cachectic patients exhibited significantly lower bacteriophage abundance, predominantly affecting Caudovirales and Siphoviridae species (double-stranded DNA) but also Inoviridae and Microviridae families (single-stranded DNA). Machine learning models exploiting the data for classification between cachectic and non-cachectic state yielded an AUC of 0.704. Caudovirales and Siphoviridae species were among the top-most important classifiers. AUC increased to 0.850 with solely antibiotic-exposed samples from 20 cachectic and 10 non-cachectic patients. This study is the first to suggest a link between cancer cachexia and intestinal bacteriophage richness. This could constitute a new basis for hypothesis-driven research in cancer cachexia diagnosis and treatment.
Comparison of mechanical properties of rabbit and human skin decellular scaffolds for alternative uses in tissue engineering
Authors: Khatat Maryam Mousavi , Roshangar Leila , Moharamzadeh Keyvan , Rad Jafar Soleimani , Mehdipour Ahmad
Published: July 2022

Objectives: In skin tissue engineering, there is a need for suitable tissue replacements that can be used as scaffolding. The higher similarity of the structural properties and biocompatibility of these scaffolds, the more desirable the alternative will be. Due to this, and considering that this scaffold should be used decellularized if needed, this study was performed on decellularized tissues. this study aimed to study the similarity of mechanical properties of rabbit decellularized derma with human decellularized derm.Methods: Sampling was done in this study as available. A total of 52 rabbit specimens were obtained, which were divided into two groups: decellularized and control. Twelve human specimens were obtained, which were decellularized and controlled. The stress strain of the samples was measured by Zwick / Roell Z010 device. Then, based on the obtained values, Young's modulus was calculated and the mean values in the groups were compared by t-test. Graphpad prism Version 9.0.0 software was used for comparison. A significance level of 0.05 was considered.Results: The results showed that there was no significant difference between the mean Young's modulus of rabbit skin samples before and after decellularization. The results also showed that there was no significant difference between the mean Young's modulus of human skin samples before and after decellularization. Finally, the results showed that there is no significant difference between the mean Young's modulus of decellularized rabbit skin and decellularized human skin.Conclusion: According to the results of this study, detoxified rabbit skin can be a suitable alternative to human skin in tissue engineering in terms of mechanical properties.
Umbilical cord plasma Lysophospholipids and Triacylglycerols Associated with Birthweight Percentiles
Authors: Wong Gerard , Narasimhan Kothandaraman , Cheong Wei Fun , Ng Sharon , Aris Izzuddin M. , Loy See Ling , Bendt Anne , Tan Kok Hian , Yap Fabian , Shek Lynette P , Chong Yap Seng , Gluckman Peter , Godfrey Keith M , Lee Yung Seng , Wenk Markus , Karnani Neerja , Chan Shiao-Yng
Published: July 2022

Dysregulated transplacental lipid transfer and fetal-placental lipid metabolism affect birthweight, as does maternal hyperglycemia. As the mechanisms are unclear, we aimed to identify the lipids in umbilical cord plasma that were most associated with birthweight.
Seventy-five Chinese women with singleton pregnancies recruited into the GUSTO mother-offspring cohort were selected from across the glycemic range based on a mid-gestation 75g oral glucose tolerance test, excluding pre-existing diabetes. Cord plasma were analysed using targeted liquid-chromatography tandem mass-spectrometry to determine relative concentrations of 404 lipid species across 17 lipid classes. Birthweights were standardized for sex and gestational age by local references, and regression analyses adjusted for maternal age, BMI, parity, mode of delivery, insulin treatment, and fasting/2h glucose, with false discovery-corrected p<0.05 considered significant. Ten lysophosphatidylcholines (LPC) and two lysophosphatidylethanolamines were positively associated with birthweight percentiles, while twenty-four triacylglycerols negatively associated with birthweight percentiles. The topmost associated lipid was LPC 20:2 [21.28 (95%CI 12.70, 29.87) percentile increase in standardized birthweight with each SD-unit increase in log10-transformed relative concentration]. Within these same regression models, maternal glycemia did not significantly associate with birthweight percentile. Specific fetal circulating lysophospholipids and triacylglycerols associate with birthweight independently of maternal glycemia, but a causal relationship remains to be established.