bluuebunny/crossref_metadata_2025 huggingface dataset:
Capacidad de agencia en la formación de ciudadanías ambientales desde la escuela rural
Authors: García-Calvo Mónica Ofelia , Garzón-Barragán Isabel
Published: September 2023

En el presente artículo de reflexión se plantea la capacidad de agencia como un punto de encuentro y articulación entre el enfoque de capacidades humanas en educación y la formación de ciudadanías ambientales. Este hace parte del proceso de análisis de una tesis de investigación de carácter doctoral que problematiza el paradigma de déficit, ampliamente documentado, sobre la educación en contextos escolares rurales, y la mirada de desarrollo y progreso dominante. En esta perspectiva, a lo largo del texto se desarrolla el significado de capacidad de agencia y la importancia de su inclusión para el análisis de un contexto escolar rural y de los procesos de formación de ciudadanías ambientales, tanto para abordar el problema de la desigualdad social como de la crisis ambiental. Como resultado de la reflexión, se plantean oportunidades de construcción de la capacidad de agencia en los individuos a partir de la creación de escenarios para el diálogo de saberes, la formación en el conocimiento sobre la acción, la resignificación del contexto y la apertura a los problemas ambientales globales, mediados por la implementación de procesos participativos y democráticos en las aulas.
El proyecto ambiental escolar (PRAE) en la construcción de comunidad: una experiencia de Educación Ambiental en contexto rural
Authors: Mendoza-Alba Cesar Augusto , Pedraza-Jiménez Yamile , Hernández-Barbosa Rubinsten
Published: September 2023

Las acciones antrópicas, producto de las diversas formas bioculturales de relacionarse con la naturaleza, la mayoría de las ocasiones se han establecido desde un paradigma hegemónico que ha generado crisis y destrucción. Esta situación requiere acciones urgentes para repensar la educación ambiental en pro de recomponer, reestablecer, retribuir o crear nuevas formas de relaciones pertinentes con el planeta, donde la escuela como escenario de encuentro de culturas tenga un papel protagónico. En este artículo se describe parte de una experiencia investigativa que tuvo como objetivo resignificar el papel del Proyecto Ambiental Escolar de la Institución Educativa San Pedro Claver, con el ánimo de favorecer acciones ambientalmente responsables con su territorio. Este estudio fue abordado desde fundamentos metodológicos propios de la investigación cualitativa, con perspectiva hermenéutica, que busca interpretar y comprender los múltiples sentidos de las acciones humanas. De esta forma, participaron estudiantes de los dos últimos años de escolaridad con edades entre 14 y 17 años y padres de familia reconocidos por ser líderes en sus comunidades y que adelantaran acciones en pro de fomentar prácticas ambientales. Se propone estrechar los vínculos entre la comunidad y la escuela a través de la creación de Escuelas de Formación Ambiental Veredal, con el ánimo de abordar las problemáticas ambientales, las cuales deben formar parte estructural del prae y desde allí ser objeto de reflexión, discusión y acción.
Tempo de permanência e motivos de retirada de cateter venoso periférico em unidade neonatal
Authors: Vilvert Gabriela , Martello Naiash Vanuzzi , Schulz Lidiane Ferreira
Published: September 2023

INTRODUÇÃO: Identificar o tempo médio de permanência do cateter venoso periférico, descrever os motivos de retirada do cateter venoso periférico dos recém-nascidos em uso de cateter venoso periférico internados em uma unidade neonatal. MÉTODOS: Pesquisa quantitativa com recém-nascidos que estiveram hospitalizados em uma Unidade Neonatal entre dezembro de 2021 a maio de 2022. Os dados foram obtidos a partir dos prontuários eletrônicos e da ficha de classificação diária dos dispositivos intravenosos periféricos preenchidos na unidade neonatal, posteriormente organizados em planilhas para a análise descritiva. RESULTADOS: O peso médio dos recém-nascidos foi de 2312,68 gramas, os principais motivos de internação foram desconforto respiratório e prematuridade. Foram 132 punções analisadas, o tempo mais frequente de permanência do cateter foi de um dia, com 40,9%; observou-se também que 9,8% não duram 24 horas. A infusão contínua é utilizada em 50,8% dos casos, sendo os principais motivos de retirada infiltração e extravasamento, enquanto que a obstrução é predominante em terapias intermitentes. CONSIDERAÇÕES FINAIS: Os cateteres apresentam baixa permanência nos neonatos e são removidos por fim de tratamento ou complicações. Identificar os riscos nos permite desenvolver barreiras de proteção na terapia intravenosa periférica e contribuir para a prática de cuidados diários.
Repercussões da COVID-19 nos profissionais da saúde do serviço de atendimento pré-hospitalar móvel: revisão integrativa
Authors: Rocha Maryelly Beatriz Martins , Dantas Dândara Nayara Azevedo , Lino Cristiane Ribeiro de Melo , E Araújo Rhayssa de Oliveira , Silva Wanessa Nayara , Das Neves Júnior Tarcísio Tércio
Published: September 2023

INTRODUÇÃO: Sumarizar o conhecimento científico sobre as repercussões da COVID-19 nos profissionais de saúde do serviço de atendimento pré-hospitalar móvel. MÉTODO: Trata-se de uma revisão integrativa realizada nas bases de dados Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), PubMed Central, SCOPUS, ScienceDirect e Web of Science. A busca ocorreu nos meses de maio e junho de 2022. A busca resultou em um total de 263 artigos e, após aplicação dos critérios de seleção, nove artigos compuseram a amostra final. RESULTADOS: As principais repercussões da COVID-19 nos profissionais da saúde do serviço de atendimento pré-hospitalar móvel corresponderam a repercussões mentais (33,3%; n=3); ocupacionais (33,3%; n=3); bem como repercussões mentais, ocupacionais e físicas (33,3%; n=3). Alguns estudos apontaram estratégias para diminuir a exposição dos profissionais ao Coronavírus, durante a pandemia da COVID-19. Nenhum estudo incluído apontou estratégias de suporte e apoio aos sintomas mentais dos profissionais. CONSIDERAÇÕES FINAIS: Os resultados permitiram concluir que a COVID-19 repercutiu na saúde mental, física e ocupacional dos profissionais do atendimento pré-hospitalar móvel, bem como ocasionou mudanças no processo de trabalho e sobrecarga ocupacional.
Didática das Ciências Sociais na Formação de Professores no Espaço Luso-Brasileiro
Authors: Hortas Maria Joao , Dias Alfredo
Published: September 2023

Este número temático dedicado à “Didática das Ciências Sociais no espaço Luso-Brasileiro”, o número 13 da REIDICS, permite alargar o nosso horizonte sobre o estudo e a reflexão que têm vindo a ser produzidos a respeito do lugar e das finalidades da Didática das Ciências Sociais, na formação inicial de professores, no Brasil e em Portugal.

Estes dois territórios, unidos e separados pelo oceano Atlântico, encetaram uma relação colonial no século XVI e constituem-se hoje como um espaço político e diplomático, social e cultural, que partilha preocupações sobre causas comuns. Entre as múltiplas partilhas, encontramos neste número da REIDICS docentes e investigadores de Ciências Sociais e da sua Didática que, dos dois lados do Atlântico, lecionam nos cursos de formação inicial de professores.
Formação inicial de professores de Geografia no Brasil: diretrizes e demandas para uma qualificação profissional
Authors: Copetti Callai Helena , De Souza Cavalcanti Lana
Published: September 2023

Este texto apresenta uma discussão sobre a formação de professores e professoras de geografia no Brasil, com foco nas políticas educacionais e normas que estão atualmente em vigor. Através de uma análise de documentos relevantes, fornecemos nossas perspectivas sobre algumas determinações delineadas na legislação. Diante da significativa desigualdade social no Brasil, agravada pela crise global da COVID-19, é crucial implementar projetos educacionais de alta qualidade que garantam o acesso à educação para todas as crianças e jovens, permitindo que vivam como cidadãos plenos. Nós afirmamos que a geografia escolar desempenha um papel vital nesse processo de formação, pois permite que os estudantes desenvolvam um modo crítico de pensar – o pensamento geográfico – que é essencial para navegar pelas complexidades da sociedade contemporânea, compreender o mundo e seu lugar nele. Argumentamos que os professores de geografia devem receber uma formação abrangente que abarque não apenas a geografia, mas também dimensões políticas e pedagógicas, e que enfatize a inseparabilidade dos aspectos teóricos e práticos.
Palavras que dão sentido à História - ideias de estudantes em finais de ciclo da escolaridade obrigatória e do Mestrado em Ensino de História
Authors: Solé Glória , Gago Marília
Published: September 2023

A História assume-se como a ciência que pretende reconstruir as realidades do passado procurando explicar as razões, as motivações e as intenções das ações e das interações entre os diversos seres humanos nos diferentes segmentos temporais, bem como dar sentido ao todo da experiência histórica em que o “eu” e o “nós” se vai reconstruindo dinamicamente no fluir temporal. Este estudo quantitativo-descritivo e interpretativo procura compreender o que é que os jovens, em diferentes estádios de formação (final do ensino básico, secundário e no mestrado de ensino de História), entendem e que ideias expressam sobre o que é a História e como esta pode ser útil para a sua vida prática. Aplicou-se um questionário, em formato online (google form) a um universo de 93 participantes (amostragem estratificada e de conveniência), estudantes de final de ciclo de estudo, em que se pedia para responder, de forma aberta, a duas questões: “Indique três palavras que podem resumir o que é para si História” e “Indique três palavras em que indiques para que serve a História”, justificando. A análise dos dados evidenciou que a maioria dos estudantes do 3.º CEB e do ensino secundário expressam uma ideia de História em linha com uma consciência histórica tradicional e exemplar, considerando que o estudo do passado é relevante para a obtenção de conhecimento, de cultura e de lições e erros. Alguns estudantes do ensino secundário considerando a História relevante como ciência, e os estudantes do ensino superior a relacionam com a memória e como essencial para a formação da consciência histórica e da identidade. Os resultados apontam para a necessidade de articular a formação dos estudantes de diferentes ciclos de estudo com as propostas emanadas da investigação na área da Educação Histórica em maior sintonia com as orientações curriculares.
Sanger Tree of Life HMW DNA Extraction: Manual Plant MagAttract v.4 v1
Authors: Jackson Benjamin
Published: September 2023

This protocol describes the manual extraction of HMW DNA from plant or fungi tissue samples from a variety of species intended for long-read sequencing, using the Qiagen MagAttract HMW DNA extraction kit. This process is effective for approximately 60% of the plant species covered by the Tree of Life Programme, and the resulting yield of CCS data from PacBio sequencing has been good. This protocol is particularly useful for samples with limited tissue availability, as it ensures the maximum amount of HMW DNA can be extracted and recovered. The output of this protocol is HMW DNA, which depending upon yield and genome size of the species, can be directed towards either HMW DNA Pooling, HMW DNA Fragmentation: Diagenode Megaruptor®3 for LI HiFi, HMW DNA Fragmentation: Diagenode Megaruptor®3 for LI PacBio or HMW DNA Fragmentation: g-Tube for ULI PacBio. This protocol was adapted from Sanger Tree of Life HMW DNA Extraction: Manual Plant MagAttract v.2/3 to further improve sample lysis. These improvements have been made through a combination of preheating the lysis buffer, delaying the addition of RNase A to later on in sample lysis and increasing centrifugation of the lysate, which have led to a reduction in tissue clumping, minimised oxidative damage of the DNA, reduced Proteinase K inhibition of RNase A and increased purity of the HMW DNA due to the exclusion of aggregated, insoluble or sedimented contaminants. Acronyms HMW: high molecular weight SPRI: solid-phase reversible immobilisation HiFi: high fidelity LI: low input ULI: ultra-low input CCS: circular consensus sequencing
Sanger Tree of Life HMW DNA Extraction: Plant Organic HMW gDNA Extraction (POE) v1
Authors: Jackson Benjamin
Published: September 2023

The Plant Organic HMW gDNA Extraction (POE) protocol acts as the Tree of Life's mid-throughput, reserve gDNA extraction procedure for recalcitrant, ‘routine failure’ species within the Plantae kingdom. Developed in-house, it is highly efficient at isolating pure, high-quality and high molecular weight (HMW) gDNA from the majority of plant species to an extent adequate for long-read sequencing. The POE protocol is divided into four stages: direct tissue lysis with an SDS-based buffer containing reducing agents to mitigate oxidative DNA damage, centrifugation and DS-protein complex precipitation using potassium acetate, gDNA isolation by two chloroform phase separations, and gDNA capture/purification using a 1X Sera-Mag™ SpeedBead and 0.45X AMPure® PB double SPRI cleanup/size selection. The protocol generally yields a sufficient quantity of high purity, ultra HMW (uHMW; 100 kb+) gDNA from 65–100 mg of fresh-frozen tissue for high-quality long-read sequencing submissions. However, outcome success is dependent on the plant species, tissue type and sample quality. The output of this protocol is uHMW gDNA, which depending upon yield and genome size of the species, can be directed towards either HMW DNA Pooling, HMW DNA Fragmentation: Diagenode Megaruptor®3 for LI PacBio or HMW DNA Fragmentation: g-Tube for ULI PacBio. Acronyms HMW: high molecular weight gDNA: genomic DNA SPRI: solid-phase reversible immobilisation LI: low input ULI: ultra-low input
Sanger Tree of Life HMW DNA Extraction: Manual Nucleated Blood Nanobind® v1
Authors: Biosciences Pacific
Published: September 2023

This protocol describes the manual extraction of HMW DNA from nucleated blood samples intended for long-read sequencing using the Nanobind® tissue kit and following the ‘Extracting HMW DNA from nucleated blood using Nanobind® kits’ procedure from PacBio. This process is effective for any species with nucleated blood within the Chordate group, covered by the Tree of Life Programme, however difficulties can arise with samples where preservation has not been optimal. The output of this protocol is high quality and quantity HMW DNA, which can be directed towards the HMW DNA Fragmentation: Diagenode Megaruptor®3 for LI PacBio protocol. Acronyms: HMW: high-molecular weight LI: low input
In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1 v2
Authors: Blanch-Asensio Marc
Published: September 2023

Protocol detailing a Gibson-assembly-based direct cloning method for Lactiplatibacillus plantarum WCFS1. Thelast stepin this version contains a supplemental video with extra context and tips, as part of the protocols.io Spotlight series, featuring conversations with protocol authors.)
Sanger Tree of Life HMW DNA Extraction: Automated Plant MagAttract v.3 v1
Authors: oatley graeme
Published: September 2023

This protocol describes the automated extraction and SPRI of HMW DNA from cryogenically homogenised plant and fungi tissue samples that are intended for long-read sequencing. It employs the Qiagen MagAttract HMW DNA extraction kit and the Thermo Fisher KingFisher™ Apex. This process is effective for a wide variety of plant species covered by the Tree of Life Programme. The output of this protocol is HMW DNA, which depending upon yield and genome size of the species, can be directed towards either HMW DNA Pooling, HMW DNA Fragmentation: Diagenode Megaruptor® 3 for LI PacBio or HMW DNA Fragmentation: g-Tube for ULI PacBio. This protocol was adapted from Sanger Tree of Life HMW DNA Extraction: Automated Plant MagAttract v.2 to include a pre-shear SPRI of the HMW DNA extracted, and was then updated to Sanger Tree of Life HMW DNA Extraction: Automated Plant MagAttract v.4 to improve sample lysis. Acronyms HMW: high molecular weight SPRI: solid-phase reversible immobilisation LI: low input ULI: ultra-low input
Initiating and Performing in vivo experiments of anti-cancer treatments in mice (General) v1
Authors: J Kimple Randall
Published: September 2023

This protocol describes our approach to setting up and designing experiments to study the effects of a cancer treatment in mice.
Sanger Tree of Life HMW DNA Extraction: Automated Plant MagAttract v.1 v1
Authors: Sheerin Edel
Published: September 2023

This protocol describes the automated extraction of HMW DNA from cryogenically disrupted plant and fungi tissue samples intended for long-read sequencing. It employs the Qiagen MagAttract HMW DNA extraction kit and the Thermo Fisher KingFisher™ Apex. This process is effective for a wide variety of plant species covered by the Tree of Life Programme. The output of this protocol is HMW DNA, which depending upon yield and genome size of the species, can be directed towards either HMW DNA Pooling or HMW DNA Fragmentation: Diagenode Megaruptor®3 for LI HiFi. This protocol was adapted from Sanger Tree of Life HMW DNA Extraction: Manual Plant MagAttract v.1 to include automation for a higher throughput of samples, and was updated to Sanger Tree of Life HMW DNA Extraction: Automated Plant MagAttract v.2 to improve sample lysis. Acronyms HMW: high molecular weight SPRI: solid-phase reversible immobilisation HiFi: high fidelity
Manual extraction of High Molecular Weight DNA from single mosquitoes using the Qiagen MagAttract HMW DNA kit v1
Authors: Teltscher Fiona
Published: September 2023

This is a protocol for the manual extraction of high molecular weight (HMW) DNA from insects. It uses the Qiagen MagAttract kit and factors in modifications described in the ChromiumTMGenome Reagent Kits User Guide pages 6-8 (https://support.10xgenomics.com/genome-exome/library-prep/doc/user-guide-chromium-genome-reagent-kit-v2-chemistry). It includes further modifications that benefited two particular needs. First, most reagents are halved per extraction (apart from beads) due to small specimen size (2 mg is a typical weight of an Anopheles mosquito, which is far less than the 25 mg of tissue the MagAttract kit can support). Second, due to this small specimen size, we need to maximize DNA yield, so we also perform a second elution to release more DNA. DNA resulting from this protocol can be further sheared and cleaned for successful PacBio HiFi sequencing. In our experience, a single fresh Anopheles gambiae mosquito weighing 2-3 mg yields about 600-800 ng of DNA (quantified using qubit HS DNA kit) using this protocol. Following shearing using G-tubes or the MegaRuptor and SPRI based clean up, we typically retain about 200 ng of sheared DNA, which is just about sufficient to reach 25x coverage PacBio HiFi of a 250 Mb genome. The quality and quantity of DNA is best when starting with a snap frozen from living specimen. However, we have also successfully extracted HMW DNA using this protocol from ethanol and DESS preserved specimens held at room temperature for as long as a week, as long as the specimen was punctured or gently squished to ensure rapid penetration of the preservative. See: Squishing insects for preservation of HMW DNA in the field https://www.protocols.io/view/squishing-insects-for-preservation-of-hmw-dna-in-t-cyp3xvqn We normally perform up to 8 DNA extractions in parallel (this will also depend on which magnetic rack is being used). Please add a comment to let the wider community know if it has worked or not on your species. NB -- an automated version of this protocol that works on the Kingfisher Apex is also available here on protocols.io
Purification of human cortex excitatory neuron nuclei from fetal and postnatal tissue using fluorescent activated nuclei sorting (FANS) in combination with a SATB2 antibody. v1
Authors: P Davies Jonathan
Published: September 2023

Increased understanding of the functional complexity of the genome has led to growing recognition about the role of epigenetic/transcriptional variation in health and disease. Current analyses of the human brain, however, are limited by the use of “bulk” tissue, comprising a heterogeneous mix of different neural cell types. Because epigenetic processes play a critical role in determining cell type-specific patterns of gene regulation it is important to consider cellular composition in regulatory genomic studies of human post-mortem tissue, and there is a need for methods to purify populations of specific cell-types. This protocol builds on a previous protocol that uses fluorescence-activated nuclei sorting (FANS) to isolate and profile nuclei from multiple different human brain cell-types from frozen post-mortem tissue. Because NeuN is not an optimal marker for neuronal nuclei from fetal cortex, we have optimized a method using a SATB2 antibody to purify nuclei from excitatory neurons in both fetal and postnatal cortex. Purified populations of nuclei are amenable to simultaneous profiling of i) DNA modifications (via bisulfite sequencing / array), ii) histone modifications (via CUT&Tag), iii) open chromatin analysis (via ATAC-seq), and iv) gene expression (via RNA-seq).
Formation Pecularities and Structural-Semantic Analysis of Adverbial Participle and Converb in the Rutul Language
Authors: Makhmudova S.M.
Published: September 2023

The verb in the Caucasian languages is a complex formation which determines a sentence’s actants form, i.e. its central part. The Rutul verb is also distinguished by the complexity of education, the development of forms, grammatical categories and features of functioning, however, verboids have not received proper here, the functional properties and syntactic features of adverbial participles and adverbial turns, which relate to a simple complicated sentence, then qualify as a subordinate clause, have not been studied either. Our work is devoted to the problems of forming the adverbial participle and the converb in the Rutul language. Rutul verb forms, lexico-semantic and functional properties of adverbs and converbs served as research material. Descriptive, component and distributive analysis were the main research methods.
Frames of TikTok’s Book Trailers
Authors: Bugaeva I.V. , Taradenko T.D.
Published: September 2023

This article poses the problem of the frame content of book trailers published on TikTok Internet platform. The aim of the research is to describe the features of TikTok’s book trailer’s frames as a genre of polycode text, which is achieved by the following tasks: to make a playlist of collected empirical material; to present an abstract analysis of scientific works devoted to the frame; to present the characteristics of TikTok’s book trailers as a genre of polycode text and its genre features; to determine the positions of TikTok’s book trailer in relation to the frame; to analyze the frame content of TikTok’s book trailer. The study material was 70 book trailers posted in the public domain on TikTok platform. These videos were placed in the form of a playlist for this study. Linguistic description and frame analysis methods were used to identify the following types of TikTok’s book trailers' frames: «key words», «plot details», «symbols». Each of them is characterized by its own characteristics of the information contained. The frames of TikTok’s book trailers reflect the structural plot elements of the primary source — the book. They arrange the voluminous information of the literary work in boxes, lining up according to the principles of logical relationship to each other. Each TikTok’s book trailer’s slot is a frame, fragments of video or audio material, sound and visual special effects; some images that are subjected to a socio-cultural factor.
Effect of human lactoferrin solution on histological changes of rabbit cornea in alkaline burns
Authors: Kolesnikov Alexander V. , Nemtsova Elena R. , Cherdantseva Tatyana M. , Kirsanova Irina V. , Kislyakova Tatyana D.
Published: September 2023

BACKGROUND: Eye burns are a severe injury, accounting for 6.1–38.4% of all ophthalmic diseases. Alkaline burns are the most common and most severe forms of eye burns.
AIM: Evaluation of the effectiveness of drug topical application based on human lactoferrin in alkaline cornea burns of the 3rd degree in the experiment.
MATERIAL AND METHODS: The study was conducted on 42 rabbits. It included two groups: the first (without treatment) — alkaline burns during therapy with water for injection (1 drop 3 times a day) — 21 rabbits, the second (experimental) — treatment with lactoferrin solution (concentration 2.5 mg/ml, 1 drop 3 times a day) — 21 rabbits. Alkaline burns were induced by applying a filter paper disc moistened with 5% sodium hydroxide solution to the cornea. 6 eyes of 3 intact animals served as controls. The effectiveness of the drug was evaluated by the rate of closure of the corneal epithelium defect, the time of suppression of the inflammatory reaction in the area of the defect and the limbus, the degree of restoration of the morphological structure of the cornea, as close as possible to the normal rabbit cornea. The obtained data were processed using the methods of variation statistics, the Statistica 10.0 software package. The significance of differences was assessed by calculating the median and interquartile interval. The critical level of significance for statistical criteria was taken as p=0.05.
RESULTS: From the 3rd day of the study, in the experimental treatment group, there was an acceleration of reepithelialization, restoration of the cornea's own substance, and a more rapid subsidence of inflammation compared to the control group. The thickness of the cornea in the center of the defect in the group without treatment was significantly higher than the values of intact animals at all periods of observation: on the 3rd day after the burn it was 615.99 [450.70–794.07] µm (p=0.000574), reached the maximum by day 7 — it was 1363.16 [907.78–1543.44] µm (p=0.000091), and by day 28 it decreased to 384.38 [376.03–398.14] µm (p=0.0000041). In the group with experimental treatment, the thickness of the cornea in the center of the defect also increased relative to intact animals, starting from the 1st day of pathology, reaching maximum values on the 3rd day — 436.70 [415.57–489.90] µm (p=0.005589). The use of lactoferrin solution in comparison with the first group led to a significant decrease in the thickness of the cornea in the center of the defect on 7th (p=0.039985) and 28th days (p=0.0443).
CONCLUSION: Local application of lactoferrin solution in alkaline burns of the cornea promotes faster regeneration of the epithelium and restoration of the stroma structure of the rabbit cornea.
The role of dehydration in the development of acute kidney injury in patients with COVID-19
Authors: Sakaeva Elvira R. , Shutov Aleksander М. , Efremova Elena V. , Rebrovskaya Mariya М. , Semenova Ekaterina S.
Published: September 2023

Background. Acute kidney injury often complicates the course of COVID-19; in many patients it develops even before hospitalization, and the reasons for its development are not sufficiently clear.
Aim. To study the role of dehydration in the development of community-onset acute kidney injury in COVID-19.
Material and methods. 329 patients with COVID-19 were examined (age 58.014.3 years, 172 men, 157 women). Acute kidney injury was diagnosed according to the Russian recommendations of 2020. To determine prerenal acute kidney injury, the ratio of blood urea nitrogen to blood creatinine was calculated, and to diagnose dehydration the calculated osmolarity of blood serum. Data are presented for a normal distribution as the arithmetic mean and standard deviation (MSD), for a non-normal distribution as a median (Me) and interquartile range (IQR). Univariate and multivariate logistic regression analyzes were used. To assess the diagnostic significance of quantitative characteristics in predicting a certain outcome, the ROC curve analysis method was used. Differences were considered statistically significant at p 0.05.
Results. Acute kidney injury was diagnosed in 70 (21.3%) patients, of which 58 (82.9%) were community-acquired. In 16 (27.6%) patients with community-onset acute kidney injury, it was of a prerenal nature, of which in 13 (81.3%) the calculated serum osmolarity exceeded 295 mOsm/L. Independent factors directly associated with prerenal prehospital acute kidney injury were estimated serum osmolarity (p 0.001), C-reactive protein level (p 0.001) and age (p=0.003) (R2=0.23, F=33,34).
Conclusion. Acute kidney injury complicates the course of COVID-19, and in most patients, it develops even at the prehospital stage. Estimated serum osmolarity is directly and independently associated with prerenal community-onset acute kidney injury, suggesting the important role of dehydration in its development.
The Role of Non-Rooted Social Institutions in the Evaluation of the Effectiveness of Regional Innovation System
Authors:   Czech University of Life Sciences Prague, Strielkowski Wadim , Kalyugina Svetlana ,   North-Caucasus Federal University, Astachova Elena ,   North-Caucasus Federal University, Mukhoryanova Oxana ,   North-Caucasus Federal University
Published: September 2023

A plethora of recent studies have highlighted the importance of non-rooted social institutions in influencing the effectiveness of regional innovation systems (RIS), and therefore the development of formal and informal organizational and institutional innovations that contribute to socio-economic development. Non-rooted social institutions refer to various organizations, networks, and collaborations that are not directly involved in research or production activities, but still exert a significant influence on regional innovation processes. These institutions can include government agencies responsible for policy-making and funding allocations for innovation programs at the regional level, professional associations promoting knowledge sharing and collaboration among industry professionals across regions, venture capital firms providing financial resources to startups and entrepreneurs, incubators and accelerators offering support services to early-stage ventures, as well as non-profit organizations focusing on community engagement and entrepreneurship education. Our paper focuses on the main rooted and non-rooted social institutions of the RIS systems using a case study of the Russian Federation. Moreover, it determines the degree of their influence on the assessment of their effectiveness. Furthermore, the paper explains that under conditions of economic stability in a region with different levels of innovative potential, the influence of different social institutions on RIS is determined differently. In conditions of economic instability, unrooted social institutions begin to play a key and decisive role. Based on the results of our study, we draw some conclusions regarding the need to take into account external socio-economic factors in the development of tools for assessing the effectiveness of regional innovation systems.
Seleção de Fungos Celulolíticos do Intestino Grosso de Borregos e Ovelhas Criados em Pastagens Tropicais
Authors: Martins Júnior Valdo Soares , Duarte Eduardo Robson , Silva Kellerson Luiz da , Freitas Cláudio Eduardo Silva , Pessoa Flávia Oliveira Abrão , Almeida Patrícia Natalícia Mendes de
Published: September 2023

O ambiente ruminal é composto por inúmeros microrganismos, onde cada um deles possui um papel fundamental no aproveitamento das dietas ingeridas pelos ruminantes, os fungos são seres unicelurares que atuam degradando as porções fibrosas dessa dieta. Objetivou-se neste estudo avaliar a atividade celulolítica (AC) de fungos micelianos aeróbios provenientes do trato digestório de ovinos. Foram identificados e avaliados 38 isolados de fungos micelianos provenientes de borregos e ovelhas, após o reisolamento, amostras desses microrganismos foram inoculadas em meio C, contendo celulose microcristalina a 1% como única fonte de carbono e incubadas em estufa a 37 °C, em triplicata, por um período de três dias. As leituras da AC foram realizadas após 24, 48 e 72 horas de incubação. Foi adicionado solução contendo vermelho congo e após 15 minutos, as placas foram lavadas com 15 mL de solução 1M de NaCl. Os índices de AC foram calculados pela razão entre o halo de degradação de celulose e o halo da colônia. Dois isolados do gênero Aspergillus e um Trichoderma sp. apresentaram índice AC maior que um, apresentando bom crescimento ao utilizarem esse polissacarídeo com única fonte de carbono. Futuros estudos devem avaliar o potencial desses fungos como probióticos na alimentação de ruminantes ou para microbiologia industrial.
Palavras-chave: Ovinos. Celulose. Trato digestório. Norte de Minas Gerais.
 Abstract
The ruminal environment is composed of numerous microorganisms, each of which plays a fundamental role in the use of the diets ingested by ruminants, fungi are unicellular beings that act by degrading the fibrous portions of this diet. The objective of this study was to evaluate the cellulolytic activity (CA) of aerobic mycelial fungi from the digestive tract of sheep. Thirty-eight isolates of mycelial fungi from lambs and sheep were identified and evaluated, after re-isolation, samples of these microorganisms were inoculated in medium C, containing 1% microcrystalline cellulose as the only carbon source, and incubated in an oven at 37 °C, in triplicate, for a period of three days. AC readings were performed after 24, 48 and 72 hours of incubation. Solution containing congo red was added and after 15 minutes, the plates were washed with 15 mL of 1M NaCl solution. CA indices were calculated by the ratio between the cellulose degradation halo and the colony halo. Two isolates of the genus Aspergillus and one Trichoderma sp. showed CA index greater than one, showing good growth when using this polysaccharide as a single carbon source. Future studies should assess the potential of these fungi as probiotics in ruminant feed or for industrial microbiology.
 Keywords: Sheep. Cellulose. Digestive tract. North of Minas Gerais.
Luminescent Media Based on Heteroligand Lanthanide Complexes and Lyotropic Mesophases for Bioimaging Application
Authors: Selivanova Nataliya M. , Melnikov Nikita A. , Kulagina Elena M. , Semenov Vyacheslav E.
Published: September 2023

New terbium and europium heteroligand complexes with substituted 1,2,3-triazole – bis-1,3-(4-methylcarboxyl-1,2,3-triazol-1-yl)xylylene have been synthesized. A study of luminescent properties showed that the complexes exhibit high optical characteristics. The luminescence efficiency of [Tb(AcAc)2(TR)(H2O)2], increases by 13 % compared to the monoligand complex [Tb(AcAc)3(H2O)2]. In order to create biocompatible systems, an approach for the solubilization of hydrophobic complexes by incorporating them into the lyomesophase structure of P123/DMSO has been developed. According to polarization optical microscopy, the hybrid system containing 0.2 % of the [Tb(AcAc)3(H2O)2] complex shows a lamellar mesophase. Fluorescence microscopy study showed that the [Eu(TTA)2(TR)(H2O)2] complex is efficiently adsorbed on the surface of the Paramecium caudatum microorganism and exhibits red luminescence allowing visualization of biological objects.
Non-Local Q-Tensor Approach for Description of Elastic Deformations of Nematic Liquid Crystals at Sub-Micron Scale
Authors: Trashkeev Sergey I. , Statsenko Pavel A. , Khomyakov Maksim N. , Shvetsov Sergey A.
Published: September 2023

Within the framework of phenomenological approach, a generalized concept of a non-local order parameter, that have the form of a traceless correlation tensor function or a tensor integral operator, is introduced. The main relationships, which determine the equilibrium orientational states and the phase transitions of a deformed nematic liquid crystal, are described. The resulting expression for the free energy is quadratic with respect to the gradients of the order parameter tensor. The limiting transition of the non-local order parameter to the local one leads to an expression that complements the classical Oseen–Frank theory of elasticity. The considered model includes eight independent elastic constants. Three of them relate to "bulk" constants analogous to Frank constants, another three ones relate to "surface" constants, and the remaining two constants determine the anisotropic effect of the gradient of the scalar order parameter on the director field. The constants are necessary for the consideration of a liquid crystal state near defects, as well as for the description of orientation effects caused by the inhomogeneity of order parameter. It is shown that in the free energy expression, the "surface" terms contribute to the director orientation in the case of a non-constant scalar order parameter, even in the approximation of rigid boundary conditions.
Evaluation of tear function and ocular surface changes in patients with pseudoexfoliation
Authors: Kushwah Swati , Thamodharan Sangeetha , Raju Kalyani
Published: September 2023

This study intends to evaluate the Tear function and ocular surface changes in patients with pseudoexfoliation.

This cross-sectional study will be conducted on a minimum of 45 patients fulfilling the inclusion criteria in the department of Ophthalmology, at tertiary care institute. Tear meniscus height (TMH), Schirmer’s test, tear breakup time (TBUT), fluorescein staining, conjunctival impression cytology were used to assess dry eye disease in pseudoexfoliation (PEX) patients.

In our study the majority of patients with PEX were >70 years and females (23) are more than males (22). According to Grading of dry eye, 41(45.5%) were moderate grade, 24(26.7%) were mild grade, 13(14.5%) were severe grade and 12 (13.3%) were normal grade. Conjunctival impression cytology, fluorescein staining, TBUT, Schirmer's tests, and TMH, all showed a statistically significant results with dry eye in the current study.

The patients with pseudoexfoliation syndrome are more prone to develop dry eye, as it causes tear film irregularities and a reduction in the number of goblet cells which is evident with conjunctival impression cytology and Tear film tests.
Dentistry and thromboembolic risk: Strategies for effective patient care
Authors: Kale Preeti Prakash , Kshirsagar Jyoti Sanjay , Vasanth Deepa , Pasha Zameer , Puthenkandathil Rahul , Shenoy Shailesh , Shah Ruhi Rajendra
Published: September 2023

Thromboembolic events represent a significant healthcare concern, encompassing conditions such as deep vein thrombosis, pulmonary embolism, stroke, and myocardial infarction. The management of patients with a history of thromboembolic events in the dental setting requires careful consideration and collaboration between dentists and healthcare providers. This review article delves into the intricate relationship between thromboembolic events and dentistry.

We explore the importance of patient assessment, emphasizing the need for a comprehensive evaluation of medical history, risk factors, and medication regimens. Special attention is given to anticoagulant therapy, and the role of International Normalized Ratio (INR) monitoring in maintaining safe levels of anticoagulation during dental procedures. We discuss the critical timing of dental interventions and the use of local hemostatic measures to minimize bleeding risk.By elucidating these key aspects, this review article aims to provide dental professionals with a comprehensive understanding of thromboembolic events in dentistry, fostering safe and effective oral healthcare for this patient population.
Wittgenstein on Truth: Some Remarks on Paragraphs 134-137 of Philosophical Investigations
Authors: Wawrzyniak Jan
Published: September 2023

The main aim of this text is to explicate what paragraphs 134-137 of Philosophical Investigations say about truth. Nevertheless, I start with a discussion of the remarks on truth contained in the Tractatus because the thoughts expressed in the latter should be read in the context of the thoughts expressed in the former. According to my interpretation of paragraphs 134-137 of the Investigations, Wittgenstein aims to show that the relationship between the concept of truth and the concept of a proposition may consist in the fact that truth and falsity, in certain language games, are constitutive elements of what a proposition is, but it must be added that these concepts cannot be comprehended independently from each other. I also come to the conclusion that in his opinion the concept of truth is expressed in various ways in our language, it being conveyed by such expressions as “… is true” and “this is how things are: …”, and that this shows that while asserting that something is true one can emphasize, on the one hand, that it is a proposition that says that things are a certain way, and on the other, just the fact that things are thus and so.
Epistemic emotions - what are they and are they exclusive to humans?
Authors: Dutkowska Anna
Published: September 2023

Najogólniej emocje epistemiczne scharakteryzować można jako emocje, które dotyczą własnych stanów i procesów umysłowych podmiotu oraz związane są z poznaniem i generowaniem wiedzy. Mogą powstawać jako efekt niezgodności poznawczej, która może pojawić się w wyniku nieoczekiwanych informacji, sprzecznych z wcześniejszą wiedzą. Mają one istotny wpływ na eksplorację i generowanie wiedzy o sobie i o świecie oraz na zmiany konceptualne czy wydajność poznawczą.
W badaniach eksperymentalnych dotyczących emocji epistemicznych brak jest perspektywy porównawczej. Na pierwszy rzut oka sytuacja ta może nie budzić kontrowersji, gdyż kategoria emocji epistemicznych została w taki sposób zdefiniowana, że wydaje się przysługiwać jedynie ludziom. Interesująca perspektywa pojawia się jednak, gdy podda się analizie badania porównawcze z zakresu etologii poznawczej, prymatologii czy psychologii porównawczej. Badacze wskazują na szereg zachowań zwierząt pozaludzkich świadczących za posiadaniem przez nie szerokiego wachlarzu emocji – w tym takich, które wyróżnia się w katalogu emocji epistemicznych, tj. zdziwienia, ciekawości czy niepewności.
Przedstawiony artykuł stanowił będzie próbę odpowiedzi na postawione w tytule pytania oraz wprowadzenie do dociekań nad kategorią emocji epistemicznych uwzględniającą rezultaty badań z zakresu wspomnianych dziedzin.
Social Ontology in the View of Roman Ingarden's Philosophy
Authors: Kosecki Artur
Published: September 2023

W ontologii społecznej prowadzi się dociekania nad tym, jak istnieją fakty społeczne i instytucjonalne, czym jest grupa społeczna, czy intencjonalność kolektywna. Fenomenologia jest jednym z XX-wiecznych nurtów filozoficznych, których wkład jej przedstawicieli nad wspomnianymi zagadnieniami należy uznać za znaczący (m.in. von Hildebrandta, Reinacha, Schelera, Stein). Celem niniejszego artykułu było rozważenie możliwego wkładu Ingardena w ontologię społeczną. Artykuł składa się z dwóch części: przeglądowej i interpretacyjnej. W pierwszej w ramach wstępu wskazałem, dlaczego fenomenologię błędnie uważa się za „aspołeczną”. W drugiej zrekonstruowałem poglądy metaontologiczne Ingardena z jego opus magnum – Sporu o istnienie świata. Zgodnie z deklaracją z tego dzieła przedmioty społeczno-prawne stanowią dziedzinę bytów o elementach heteronomicznych i czysto intencjonalnych. W artykule ukazałem, że Ingarden explicite nie formułuje teorii bytu społecznego, ale na kanwie jego ontologii szkic takiej można spróbować przedstawić. Uznałem, że w celu realizacji tego zamierzenia należy podjąć próbę nakreślenia różnicy między przedmiotami fikcji, a przedmiotami społeczno-prawnym w ramach jego ontologii.
Moral Attitudes in Poland: “Towards”, “Away” and “Against People” – Socio-Cultural Determinants and Religious Implications. Based on European Values Study 2017
Authors: Szczygielski Dominik
Published: September 2023

According to the latest research institutional trust may serve as a significant predictor of prosociality. The main purpose of this article is to examine types of trust as predictors of moral attitudes “towards”, “away” and “against” people. Another aim is to examine institutional trust as a predictor of moral attitudes in correlation to other significant predictors, like religiousness, education and size of town. This paper presents analysis in the course of which interpersonal and institutional trust has been confirmed as alternative (potentially competing) predictors of moral attitudes than other established variables, e.g., religiousness. Presented findings allow to pose the question about the future of religion in stable, democratic societies.
Talking and Acting A Pandemic Ethnography of COVID-19 in Montmartre
Authors: Black Alexis
Published: September 2023

Informed by eighteen months of ethnographic fieldwork in Montmartre, one of the last village-like neighbourhoods in Paris, in this paper, I analyze how people in this community talked through and acted out the COVID-19 pandemic. Using theoretical frameworks from linguistic, cognitive and medical anthropology, I examine “small stories” (Georgakopoulou 2007) about COVID-19, in particular, the analogical and conceptual aspects of this talk. How do people construct understandings of crisis as it evolves? What does this process look like when talk becomes action and reaction and what does it say about the future? This paper explores how people employed analogy, cultural scripts and other linguistic wor(l)d-building tools in their talk about their experiences and comprehensions of COVID-19. Following the arguments of Ochs (2012), I propose that talking about COVID-19 is itself an experience of the virus, an experience that informs people’s understandings of their present circumstances and future possibilities.
Nonlinear modeling of carbon dynamics in soil treated with tannery sludge
Authors: Leoncio da Silva Maylon , Silva Edilson Marcelino , Campos Frühauf Ariana , Augusto Muniz Joel , Jesus Fernandes Tales
Published: September 2023

In 2020, the Brazilian commercial cattle herd was the largest in the world, representing 14.3% of the worldwide herd, with201.7 million heads. Although this activity yields significant profits, contributing to the economic and social development ofBrazil, it has been the target of concerns, mainly due to the large production of waste/effluents associated to bovine leatherprocessing. In this scenario, mineralization is important because nutrients essential for plant growth are released duringthe process of organic waste decomposition, and the dynamics of carbon release can be described by nonlinear regressionmodels. Thus, this study aimed to model the mineralization of organic carbon in the soil for 6, 12, 24, and 36 megagramsdoses per hectare (Mg ha−1) of tannery sludge using the Stanford & Smith, Cabrera, and Juma nonlinear models. Very clayeysoil samples were used: eutroferric red nitosol (NVef). Mineralized carbon was measured in 21 observations over time untilthe 105th day of incubation. Parameters were estimated using the least squares method. Adjustments were compared usingthe corrected Akaike information criterion (AICc) and the adjusted coefficient of determination (R2aj) as selection criteria. The Cabrera model had the best adjustments for doses 6, 12, and 24 Mg ha−1 and Juma for dose 36 Mg ha−1, based on the selection criteria used. Although the Stanford & Smith model is the most widely used in the literature to model soil carbon dynamics, its use was not the most appropriate for any of the doses evaluated in this study. The higher the dose of tannerysludge, the greater the amount of potentially mineralizable carbon.