bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Identification and Expression Analyses of the Cotton AGO Genes and Their Potential Roles in Fiber Development and Stress Response
Authors: Fu Mingchuan , Chen Yizhen , Li Hao , Wang Liguo , Liu Renzhong , Liu Zhanji
Published: August 2022

Argonaute proteins (AGOs) are indispensable components of RNA silencing. However, systematic characterization of the AGO genes have not been completed in cotton until now. In this study, cotton AGO genes were identified and analyzed with respect to their evolution and expression profile during biotic and abiotic stresses. We identified 14 GaAGO, 14 GrAGO, and 28 GhAGO genes in the genomes of Gossypium arboreum, Gossypium raimondii, and Gossypium hirsutum. Cotton AGO proteins were classified into four subgroups. Structural and functional conservation were observed in the same subgroups based on the analysis of the gene structure and conserved domains. Twenty-four duplicated gene pairs were identified in GhAGO genes, and all of them exhibited strong purifying selection during evolution. Moreover, RNA-seq analysis showed that most of the GhAGO genes exhibit high expression levels in the fiber initiation and elongation processes. Furthermore, the expression profiles of GhAGO genes tested by quantitative real-time polymerase chain reaction (qPCR) demonstrated that they were sensitive to Verticillium wilt infection and salt and drought stresses. Overall, our results will pave the way for further functional investigation of the cotton AGO gene family, which may be involved in fiber development and stress response.
Cathepsin D Plays a Vital Role in Macrobrachium nipponense of Ovary Maturation: Identification, Characterization, and Function Analysis
Authors: Cheng Dan , Zhang Wenyi , Jiang Sufei , Xiong Yiwei , Jin Shubo , Pan Fangyan , Zhu Junpeng , Gong Yongsheng , Wu Yan , Qiao Hui , Fu Hongtuo
Published: August 2022

The oriental river prawn Macrobrachium nipponense is an economically important aquacultural species. However, its aquaculture is negatively impacted by the rapid sexual maturation of female M. nipponense. The fast sexual maturation produces a large number of offspring which leads to a reduction in resilience, a low survival rate, and an increased risk of hypoxia, this in turn, seriously affects the economic benefits of prawn farming. Cathepsin D is a lysosomal protease involved in the ovarian maturation of M. nipponense. In the current study, the cDNA of the gene encoding cathepsin D (Mn-CTSD) was cloned from M. nipponense. The total length was 2391 bp and consisted of an open reading frame (ORF) of 1158 bp encoding 385 amino acids. Sequence analysis confirmed the presence of conserved N-glycosylation sites and characteristic sequences of nondigestive cathepsin D. The qPCR analysis indicated that Mn-CTSD was highly expressed in all tissues tested, most significantly in the ovaries, whereas in situ hybridization showed that expression occurred mainly in oocyte nuclei. Analysis of its expression during development showed that Mn-CTSD peaked during the O-IV stage of ovarian maturation. For the RNAi interference experiment, female M. nipponense specimens in the ovary stage I were selected. Injection of Mn-CTSD double-stranded (ds)RNA into female M. nipponense decreased the expression of Mn-CTSD in the ovaries, such that the Gonad Somatic Index (GSI) of the experimental group was significantly lower than that of the control group (1.79% versus 4.57%; p < 0.05). Ovary development reached the O-III stage in 80% of the control group, compared with 0% in the experimental group. These results suggest that Mn-CTSD dsRNA inhibits ovarian maturation in M. nipponense, highlighting its important role in ovarian maturation in this species and suggesting an approach to controlling ovarian maturation during M. nipponense aquaculture.
Comparison of Academic Motivation between Business and Healthcare Students in Online Learning: A Concurrent Nested Mixed-Method Study
Authors: Kotera Yasuhiro , Gorchakova Valentina , Maybury Sarah , Edwards Ann-Marie , Kotera Hiromasa
Published: August 2022

While the demand for online education and the diversity of online students have been increasing worldwide, how online students motivate themselves to continuously engage in learning remains to be appraised. Research in the face-to-face contexts reports that academic motivation is central to student success and wellbeing, and the type of motivation can differ by subject. In particular, the motivation of business students and healthcare students can differ considerably. This study aimed to understand the motivation of online students, and compare them between business and healthcare students using a concurrent nested mixed-method design with correlation and thematic analyses. A survey regarding motivation, learning enjoyment, and study willingness was responded to by 120 online students (61 business and 59 healthcare). Business students were associated with extrinsic motivation, whereas healthcare students were associated with intrinsic motivation. While students in both groups enjoyed the pursuit of knowledge, healthcare students valued the process and accomplishment, whereas business students regarded education as steppingstones in their careers. Findings can help educators develop effective motivational support for these student groups.
A Unique Glassy Cell Carcinoma (GCC) of the Cervix Diagnosed during Pregnancy—A Case Report
Authors: Grabowska Marlena , Baum Ewa , Lewandowska Małgorzata , Sajdak Stefan , Dolińska-Kaczmarek Klaudia , Englert-Golon Monika
Published: August 2022

Glassy Cell carcinoma (GCC) of the cervix is classified as a unique, aggressive neoplasm, with different sensitivity to chemotherapy and radiotherapy. It is such an extremely rare tumor that it is practically not observed during pregnancy. Information on the coexistence of cervical GCC with pregnancy is also unique, so it seems extremely important to disseminate it in order to develop the most effective treatment regimen. Additionally, making any decisions regarding therapeutic methods during pregnancy encounters great ethical problems. We present the case of a 26-year-old pregnant woman, 18 weeks gestation, diagnosed with GCC of the cervix, IB3 grade in the International Federation of Gynecology and Obstetrics (FIGO) scale. Despite the unfavorable prognosis, the use of chemotherapy in a pregnant patient brought on a favorable therapeutic effect, without any negative effects on the fetus. The article also presents a literature review on the epidemiology, pathology, immunohistochemistry, treatment and prognosis of this rare disease.
Sleep Quality Worsens While Perceived Stress Improves in Healthcare Workers over Two Years during the COVID-19 Pandemic: Results of a Longitudinal Study
Authors: Jahrami Haitham , Haji Eman A. , Saif Zahra Q. , Aljeeran Noora O. , Aljawder Aysha I. , Shehabdin Faisal N. , Fekih-Romdhane Feten , Trabelsi Khaled , BaHammam Ahmed S. , Vitiello Michael V.
Published: August 2022

The purpose of the study was to measure changes in sleep quality and perceived stress and their interrelationships in a sample of healthcare workers two years post the COVID-19 pandemic. Using a cohort design, data were collected from frontline healthcare workers (FLHCW, n = 70) and non-frontline healthcare workers (NFLHCW, n = 74) in April 2020 (T1) and in February 2022 (T2). The Pittsburgh Sleep Quality Index (PSQI) and the Perceived Stress Scale (PSS-10) were administered at both time points. There were no differences in sleep quality or perceived stress between FLHCW and NFLHCW at either timepoint. For the entire sample, the PSQI scores at T2 were significantly higher than at T1 (7.56 ± 3.26 and 7.25 ± 3.29, respectively) (p = 0.03, Cohen’s d = 0.18). PSS-10 scores at T2 were significantly lower than at T1 (19.85 ± 7.73 and 21.13 ± 7.41, respectively) (p = 0.001, Cohen’s d = 0.78). Baseline sleep quality PSQI (T1) was a significant predictor for changes in sleep quality. During the initial months of the outbreak of the COVID-19 pandemic, poor sleep quality and perceived stress were common for healthcare workers. Two years into the pandemic, the perceived stress was reduced, but sleep quality worsened.
Vibrio parahaemolyticus Is Associated with Diarrhea Cases in Mexico, with a Dominance of Pandemic O3:K6 Clones
Authors: León-Sicairos Nidia , Zatarain-Lopez Ricardo , Angulo-Zamudio Uriel , Velazquez-Roman Jorge , Flores-Villaseñor Héctor , Martinez-Garcia Jesus , Moreno-Pérez María , Buelna-Romero Alma , Hernández-Monroy Irma , Lopez-Martinez Irma , Cuen-Diaz Hector , Diaz-Quiñonez José , Canizalez-Roman Adrián
Published: August 2022

In the present study, we conducted surveillance of the V. parahaemolyticus strains present in clinical samples from six geographical regions of Mexico (22 states) from 2004 to 2011. The serotype dominance, virulence genes, presence of pandemic O3:K6 strains, and antibiotic resistance of the isolates were investigated. In total, 144 strains were isolated from the clinical samples. Seven different O serogroups and twenty-five serovars were identified. Most clinical isolates (66%, 95/144) belonged to the pandemic clone O3:K6 (tdh+, toxRS/new+ and/or orf8+) and were detected in 20 of the 22 states. Among the pandemic clones, approximately 17.8% (17/95) of the strains cross-reacted with the antisera for the K6 and K59 antigens (O3:K6, K59 serotype). Other pathogenic strains (tdh+ and/or trh+, toxRS/new−, orf8−) accounted for 26.3%, and the nonpathogenic strains (tdh− and/or trh−) accounted for 7.6%. Antimicrobial susceptibility testing showed that most of the strains were resistant to ampicillin (99.3%) but were sensitive to most tested antibiotics. The level of multidrug resistance was 1.3%. Our results indicate that pandemic O3:K6 is present in most Mexican states, thus, constant surveillance of V. parahaemolyticus strains in diarrhea patients is a public health priority and is useful for conducting risk assessments of foodborne illnesses to prevent V. parahaemolyticus outbreaks. Overall, our observations indicate that the pandemic O3:K6 clone of V. parahaemolyticus has become a relatively stable subpopulation and may be endemically established in Mexico; therefore, constant surveillance is needed to avoid new outbreaks of this pathogen.
Cross-Sectional Study of Factors Influencing Perceived Threat and Stress among the Arab Minority in Israel during the COVID-19 Pandemic
Authors: Ali-Saleh Ola , Halperin Ofra
Published: August 2022

This study aimed to examine the impact of the locus of control, coping strategies and loneliness on perceived threat and stress among the Arab minority in Israel during the first COVID-19 closure. This was a cross-sectional online study, with 486 participants who completed a questionnaire measuring the study variables during the period of 28–31 March 2020. Statistical analyses included t-tests and analyses of variance. Means, standard deviations and intercorrelations for the study variables were calculated. The results revealed a moderate-low level of stress and a moderate-high level of perceived threat. Higher stress was related to higher perceived threat, a greater external locus of control, lower problem-focused coping, higher emotion-focused coping and social support-seeking and higher loneliness. Perceived threat was positively related to both problem-focused coping and emotion-focused coping. The results show that the Arab population in Israel coped as a strong minority group. This study contributes to our understanding of how minority groups cope in the current epidemic and to the identification of effective strategies for reducing stress during this challenging period. The study’s results may help devise intervention programs that foster more effective coping capabilities among this and other minority populations.
What Drives Abdominal Obesity in Peru? A Multilevel Analysis Approach Using a Nationally Representative Survey
Authors: Hernández-Vásquez Akram , Olazo-Cardenas Kamyla M. , Visconti-Lopez Fabriccio J. , Barrenechea-Pulache Antonio
Published: August 2022

Abdominal obesity (AO) is a serious public health threat due to its increasing prevalence and effect on the development of various non-communicable diseases. A multilevel analysis of the 2019 Demographic and Family Health Survey (ENDES in Spanish) using the Latin American Diabetes Association (ALAD in Spanish) cut-off points was carried out to evaluate the individual and contextual factors associated with AO in Peru. A total of 30,585 individuals 18 years and older were included in the analysis. The prevalence of AO among Peruvians in 2019 was 56.5%. Individuals of older age (aOR 4.64; 95% CI: 3.95–5.45), women (aOR 2.74; 95% CI: 2.33–3.23), individuals with a higher wealth index (aOR 2.81; 95% CI: 2.40–3.30) and having only secondary education (aOR 1.45; 95% CI: 1.21–1.75) showed increased odds of presenting AO compared to their peers. At a contextual level, only the Human Development Index (aOR 1.59; 95% CI: 1.17–2.16) was associated with the development of AO. A high Human Development Index is the contextual factor most associated with AO. It is necessary to formulate and implement new public health policies focused on these associated factors in order to reduce the prevalence of OA and prevent the excessive burden of associated noncommunicable diseases.
Supporting the Community to Embrace Individuals with Dementia and to Be More Inclusive: Findings of a Conceptual Framework Development Study
Authors: Bressan Valentina , Snijder Allette , Hansen Henriette , Koldby Kim , Andersen Knud Damgaard , Allegretti Natalia , Porcu Federica , Marsillas Sara , García Alvaro , Palese Alvisa
Published: August 2022

The number of community-dwelling people with dementia (PwD) is rising, and the role of their relatives is crucial in addressing and mitigating the implications of dementia on health care systems and on society. We developed a new conceptual framework to promote the collaboration of the community in supporting relatives who are caring for a PwD as well as a range of stakeholders in embracing dementia. A qualitatively driven, multi-method study divided into three phases was performed from 2019 to 2021. A qualitative descriptive study, a mixed-method systematic review and three consensus workshops were conducted, and their results were triangulated. The final version of the Community Collaboration Concept Framework is composed of three main domains based upon seven components: (1) embracing dementia; (2) creating empowerment and a sense of community; (3) collaborating through cocreation and design thinking. The new framework is based on the literature, the synthesis of empirical data and the consensus of a panel of international experts, supporting the global goal of improving community inclusiveness and collaboration. Further studies are needed to confirm its validity, how it should be implemented in practice in various settings and to propose improvements when designing projects based upon it.
Student Evaluation of Distance Learning during the COVID-19 Pandemic: A Cross-Sectional Survey on Medical, Dental, and Healthcare Students at Sapienza University of Rome
Authors: Lollobrigida Marco , Ottolenghi Livia , Corridore Denise , Pingitore Gianluca , Damiano Cecilia , Serafini Giorgio , De Biase Alberto
Published: August 2022

The COVID-19 pandemic has had a deep impact on university education, necessitating an abrupt shift from face-to-face learning to distance learning (DL). This has created new challenges, especially for those courses in which practical activities and internships are integral parts of the education program. The aim of this study was to assess the impact of DL on the study progress of a population of pregraduate students of medicine, dentistry, and healthcare professions. The survey was administered through an anonymous questionnaire by sharing a Google Forms link. Demographic data and educational background information were collected to obtain a profile of the participants. Different aspects of DL were investigated, including availability of digital devices, quality of connection, and environmental conditions; other questions focused on the effects of DL on students’ progress and professional maturation. Measures of association were also calculated using the chi-squared test, Cramer V, and Somers D. Among the 372 who participated, the results showed that students had a positive attitude toward online classroom and that DL did not substantially affect their progress. Most of the associations were statistically significant, also highlighting the effect of the degree course on the responses. Some critical issues clearly emerged, however, including the lack of adequate devices and environmental conditions due to economic disparity, poor relationships, suspension of internship programs, and clinical training. The results suggest that DL cannot be considered as a substitute for classroom-based medical education outside an emergency context.
How Did Work-Related Depression, Anxiety, and Stress Hamper Healthcare Employee Performance during COVID-19? The Mediating Role of Job Burnout and Mental Health
Authors: Sun Jianmin , Sarfraz Muddassar , Ivascu Larisa , Iqbal Kashif , Mansoor Athar
Published: August 2022

The study objective was to examine the psychological impact of the COVID-19 pandemic on the performance of healthcare employees. The study was informed by a theoretical framework that incorporates different psychological issues (i.e., stress, depression, and anxiety) that influence healthcare workers’ performance through the mediating roles of job burnout and mental health. The study data was gathered through structured questionnaires from 669 participants working in the healthcare sector in Pakistan. A structured equation modeling (SEM) technique was used for data analysis and hypothesis development. It was found that stress, depression, and anxiety positively affected healthcare employees’ job performance during COVID-19. Psychological factors had a positive and significant impact on job burnout and mental health. Job burnout and mental health mediated the relationship between stress, anxiety, depression, and employee performance. The ongoing repercussions of COVID-19 include their impact on employee performance in the healthcare sector. Healthcare worker performance is critical to fostering industrial economic growth. Elevated levels of stress, depression, and anxiety have profoundly exacerbated employee mental health issues. COVID-19 has created challenging working conditions in organizations requiring that they address the growing psychological issues which impact negatively on worker performance.
Depressive and Anxiety Symptoms and Their Relationships with Ego-Resiliency and Life Satisfaction among Well-Educated, Young Polish Citizens during the COVID-19 Pandemic
Authors: Goryczka Agnieszka , Dębski Paweł , Gogola Anna M. , Gorczyca Piotr , Piegza Magdalena
Published: August 2022

Ego-resiliency is a set of traits that promotes positive adaptation to life’s vicissitudes. High ego-resiliency helps in upholding one’s personality system when facing adversity and in adjusting it to new environmental demands. Our study aimed at evaluating the connections between ego-resiliency, the severity of anxiety and depressive symptoms as well as life satisfaction during the COVID-19 pandemic in Poland. A total of 604 Polish citizens aged 16 to 69 years participated in the online survey. Ego-resiliency was measured with the Ego Resiliency Scale (ER89-R12), anxiety and depression with the Hospital Anxiety and Depression Scale (HADS), and life satisfaction with the Satisfaction with Life Scale (SWLS). Statistical analyses were performed using the Spearman rank correlation coefficient. The results revealed correlations between the intensity of depressive and anxiety symptoms, life satisfaction, and the intensity of ego-resiliency. Individuals with a high level of ego-resiliency tended to experience a lower intensity of anxiety and depressive symptoms during the COVID-19 pandemic. Moreover, individuals with a high level of ego-resiliency exhibited a higher level of life satisfaction. Our conclusions might assist in better understanding the close link between levels of ego-resiliency, the occurrence of depressive and anxiety symptoms, and satisfaction with life among Polish individuals experiencing crises.
Evaluating the Distribution of Perfluoroalkyl Substances in Rice Paddy Lysimeter with an Andosol
Authors: Eun Heesoo , Yamazaki Eriko , Pan Yu , Taniyasu Sachi , Noborio Kosuke , Yamashita Nobuyoshi
Published: August 2022

The properties of potential emerging persistent contaminants, perfluoroalkyl substances (PFAS), in an andosol rice paddy lysimeter were analyzed to determine their mobility and leaching behavior regarding carbon chain length and functional groups. For this purpose, simulated contaminated water (ΣPFAS = 1,185,719 ng/L) was used in the lysimeter. The results showed that PFAS distribution in the paddy soil lysimeter was influenced by the migration of these substances into irrigation water and their adsorption into the soil. PFHxS (C6) and PFOS (C8), which are the main components of the simulated contaminated water, were mostly captured in the soil layers of the low-humic andosol layer (0–35 cm). PFAS distribution may depend on soil properties, such as total carbon (TC) content. Compared with perfluoroalkane sulfonic acids (PFSAs), the distribution of perfluoroalkyl carboxylic acids (PFCAs) in soil showed significant variation. The remaining PFCAs were distributed across all layers of the lysimeter, except for the longer-chain PFCAs. Moreover, the PFSA distribution was directly correlated with the carbon chain number, whereby longer- and shorter-chain PFSAs accumulated in the top and bottom soil layers, respectively. This study provides detailed information on the distribution, leaching, uptake, and accumulation of individual PFAS in andosol paddy fields in Japan.
Determinants of Suicide Ideation in the Romanian Population
Authors: Vuscan Mihaela Elvira , Faludi Cristina , Rusu Sergiu Ionica , Vica Mihaela Laura , Balici Stefana , Siserman Costel Vasile , Coman Horia George , Matei Horea Vladi
Published: August 2022

Background: Suicide ideation and behaviors are directly linked to the risk of death by suicide. In Romania, as well as worldwide, increased suicide rates were observed in the recent past, more so in the context of the COVID-19 pandemic. The purpose of this study was to investigate the influence of psychosocial factors, quality of life (QOL), and loneliness dimensions and adverse life antecedents on suicide ideation (SI) and prolonged sadness (PS). Methods: This cross-sectional quantitative research study used a CATI data gathering method to investigate 1102 randomly selected individuals over 18 years of age regarding various determinants of SI and PS. Data were collected in June 2021. Descriptive, inferential, and multivariate statistics were used for data analysis. Results: SI was negatively correlated with all the assessed psychosocial factors, more significantly with family relationships, wealth, health, social relationships, and affective life. Stronger correlations were observed when investigating the state of prolonged sadness, sex, and affective lives along with health and income, which were more influential. SI was negatively correlated with QOL and positively correlated with adverse life events and total loneliness scores. Lesser educated youngsters with reduced overall happiness and a history of depression, self-harm, and trauma were at greater risk of developing SI. Conclusions: This is the first national study exploring the suicide ideation and prolonged sadness in relation to psychosocial factors, quality of life, and adverse life events. These results have important implications for suicide prevention programs, which should be designed in accordance with similar studies.
Distribution Characteristics and Restoration Application of Vegetation in Chengcun Bay Surrounding Areas of Yangjiang City
Authors: Chen Shan , Sun Yuanmin , Tang Kunxian , Zhang Fei , Ding Weilun , Wang Ao
Published: August 2022

In recent years, global warming and sea level rise have further aggravated the risk of coastal erosion. Coastal vegetation plays an important role in resisting storm surges and alleviating coastal erosion. Therefore, screening plant species for the purpose of constructing ecological seawalls to protect or repair damaged coastal zones has become a hot issue. In this paper, a field survey was conducted to investigate the vegetation in Chengcun Bay surrounding areas of Yangjiang City by combining a line survey and sample plot survey. By understanding the vegetation types, distribution and community structure in the bay’s surrounding areas and analyzing the restricting environmental factors of those plants, we put forward some countermeasures for coastal vegetation restoration in difficult site conditions from the aspects of plant species selection, vegetation configuration and restoration technology, so as to provide reference for ecological vegetation restoration in similar locations.
Public Health Management of the COVID-19 Pandemic in Australia: The Role of the Morrison Government
Authors: Duckett Stephen
Published: August 2022

The Australian Commonwealth government has four health-related responsibilities during the SARS-CoV-2 pandemic: to provide national leadership; to manage external borders; to protect residents of residential aged care facilities; and to approve, procure and roll-out tests and vaccines. State governments are responsible for determining what public health measures are appropriate and implementing them—including managing the border quarantine arrangements and the testing, tracing, and isolation regime—and managing the hospital response. This paper analyses the national government’s response to the pandemic and discusses why it has attracted a thesaurus of negative adjectives.
Effects of Acute High-Intensity Interval Exercise and High-Intensity Continuous Exercise on Inhibitory Function of Overweight and Obese Children
Authors: Zhang Ligong , Wang Dongshi , Liu Siwen , Ren Fei-Fei , Chi Lin , Xie Chun
Published: August 2022

This study aimed to examine whether a single bout each of high-intensity interval exercise (HIIE) and high-intensity continuous exercise (HICE) could improve inhibitory functions of overweight and obese children, and which mode of exercise was more beneficial. Seventy-two overweight and obese children, with (26.02 ± 1.05 kg/m2), aged 10–14 years (11.56 ± 1.03 years), were randomly assigned to three groups. The HIIE group completed a 30-min treadmill exercise session (5-min warm up, 20-min HIIE, and 5-min cool-down). The HICE group performed 30 min of rope skipping, while the control (CON) group watched a designated cartoon on a tablet computer for the same duration. Reaction time and number of errors in the Stroop test were determined before and after the intervention. The difference between pre- and post-test reaction time scores was higher in the HIIE and HICE groups than in the CON group, while the pre- and post-test difference in the number of errors was similar between groups. Overall, it is likely that both acute HIIE and HICE were similarly efficient in facilitating cognitive and inhibitory functions of children with overweight and obesity conditions, supporting the benefits of acute high-intensity exercise probability for cognitive functions of children in general, as well as of the population with overweight and obesity conditions.
A Method for Modeling the Individual Convenient Zone of a Human
Authors: Branowski Bogdan , Zabłocki Marek , Kurczewski Przemysław , Sydor Maciej
Published: August 2022

When designing products to fit a specific user, it is essential to know the user’s upper limb range and strength capabilities at each point of the range space. This is particularly relevant when those capabilities are atypical, e.g., in cases of nonstandard body dimensions, disability, or old age. In this paper, we describe a new method to measure and model the strength capabilities at each point of any person’s upper limb range and then present this information in the form of an Individual Convenient Zone (ICZ) model, which is helpful in virtual product prototyping (CAD) for a specific user. The proposed new method includes creating a database of multiple, detailed, spatial-force characteristics, quickly identifying and modeling the ICZ of any human, and analyzing the ergonomics of a product using a digital human model in combination with the ICZ model. The paper also describes an example of how the proposed methodology can be used to customize kitchen furniture design to the ICZ of a specific senior. The expected result of incorporating ICZ into the design is a better fit between the designed product and the user’s needs, supporting user-centered design methodology. Using ICZ enables the involvement of end-users in product design (participatory design). This is particularly important when designing for people with mobility impairments who are more sensitive to nonergonomic solutions. The ICZ modeling method described in this article may have broader applications beyond kitchen furniture design; it could be used to design workspaces and other similar areas where humans reside and perform manipulation activities.
Hypomineralized Teeth and Their Impact on Oral-Health-Related Quality of Life in Primary School Children
Authors: Reissenberger Tim , Ebel Markus , Klode Christian , Hirsch Christian , Bekes Katrin
Published: August 2022

Background: Molar–incisor hypomineralization (MIH) has a strong negative effect on oral-health-related quality of life (OHRQoL). Malformed teeth can be hypersensitive, and the discoloration might affect children’s appearances, reducing their well-being. The purpose of the study was to investigate how hypomineralized incisors and molars differ in children’s perceived OHRQoL. Materials and Methods: 252 children aged 7–10 years old were included and subdivided into three equal groups (n = 84). Group A included children with asymptomatic molars and affected incisors. Group B included children presenting only affected molars. Group C was the control group, with children showing no MIH. All participants were asked to complete the German version of the Child Perceptions Questionnaire (CPQ-G8-10) to measure OHRQoL. Results: Participants in the posterior group showed a median total CPQ of 13.4 (±1.7), which was significantly higher than scores in the anterior and control group, which showed a median total CPQ of 8.4 (±1.4) and 4.2 (±0.7), respectively. Children in the posterior group suffered more from oral symptoms and functional limitations, whereas the anterior group dealt more with social and emotional well-being problems. Conclusions: The position of the MIH-affected teeth causes different influences on perceived OHRQoL.
Acute and Subchronic Oral Toxicity of Anthraquinone in Sprague Dawley Rats
Authors: Qu Jingjing , Pei Lanjie , Wang Xiangyan , Fu Shaohua , Yong Ling , Xiao Xiao , Xie Qianqian , Fan Bolin , Song Yan
Published: August 2022

Objective: This study was conducted to evaluate the acute and subchronic toxicity of anthraquinone. An acute toxicity test was performed in female Sprague Dawley (SD) rats, and the oral median lethal dose (LD50) of anthraquinone was estimated to be >5000 mg/kg body weight (BW). In the subchronic study, groups of 10 male and 10 female rats were dosed with anthraquinone by gavage at 0, 1.36, 5.44, 21.76, and 174.08 mg/kg BW, 7 days/week for 90 days followed by a recovery period of 28 days. No appreciable toxic-related changes were observed in the 1.36 mg/kg BW group. When the animals received 5.44 mg/kg BW or more of anthraquinone, hyaline droplet accumulation in the renal tubules was observed in both the male and female rats, and anemia was observed in the females. When the anthraquinone dose reached 174.08 mg/kg BW, mild hepatocellular hypertrophy around the central vein of the hepatic lobule and hypothyroidism were observed in the female rats. During the recovery period, changes in clinical symptoms and parameters were considerably alleviated. Based on the results of this study, the no observed adverse effect level (NOAEL) for anthraquinone in rats was set at 1.36 mg/kg BW, and the lowest observed adverse effect level (LOAEL) was 5.44 mg/kg BW.
Succession of the Abandoned Rice Fields Restores the Riparian Forest
Authors: Lim Bong Soon , Seol Jaewon , Kim A Reum , An Ji Hong , Lim Chi Hong , Lee Chang Seok
Published: August 2022

The vegetation changes in the abandoned rice fields with different abandonment histories were analyzed across the country of South Korea. The successional process was confirmed by changes in vegetation profiles and species composition. The vegetation profile showed the process of starting with grassland, passing through the shrub stage, and turning into a tree-dominated forest. DCA ordination based on vegetation data showed that the process began with grasslands consisting of Persicaria thunbergii, Juncus effusus var. decipiens, Phalaris arundinacea, etc., then partially went through shrubland stages consisting of Salix gracilistyla, S. integra, young Salix koreensis, etc., and ultimately changed to a Salix koreensis dominated forest. In order to study the relationship between the succession process of the abandoned rice paddies and riparian vegetation, information on riparian vegetation was collected in the same watershed as the abandoned rice paddies investigated. Riparian vegetation tended to be distributed in the order of grasslands consisting of Phragmites japonica, Miscanthus sacchariflorus, P. arundinacea, etc., shrubland dominated by Salix gracilistyla, S. integra, etc., and a S. koreensis community dominated forest by reflecting the flooding regime as far away from the waterway. The result of stand ordination based on the riparian vegetation data also reflected the trend. From this result, we confirmed that the temporal sequence of the vegetation change that occurred in the abandoned rice fields resembled the spatial distribution of the riparian vegetation. Consequently, succession of the abandoned rice fields restored the riparian forest, which has almost disappeared in Korea and other Asian countries that use rice as their staple food.
Impact of COVID-19 on Health-Related Quality of Life: A Longitudinal Study in a Spanish Clinical Sample
Authors: Rodríguez-Galán Irene , Albaladejo-Blázquez Natalia , Ruiz-Robledillo Nicolás , Pascual-Lledó José Francisco , Ferrer-Cascales Rosario , Gil-Carbonell Joan
Published: August 2022

SARS-CoV-2 respiratory infection and the course of its sequelae remain to be defined. The aim of this study is to analyze health status and Health-Related Quality of Life (HRQoL) in a Spanish sample of survivors of coronavirus disease 2019 (COVID-19) pneumonia. Methods: Prospective observational study of patients who survived SARS-CoV-2 pneumonia, between February 2020 and May 2020, with systematic evaluation at 3 and 12 months after the onset of the disease. The data were obtained by reviewing the clinical history and performing a physical examination, a chest X-ray, and a pulmonary function test on the patients. Additionally, the SF-36 questionnaire was administered for the HRQoL study. Results: In total, 130 patients aged 55.9 ± 15.9 years were included. Dyspnea (36.9%) and asthenia (36.2%) were the most frequent persistent symptoms. Fibrotic pulmonary changes were detected in 20.8% of the participants. Compared to the general population, significant deterioration was detected in all domains of the SF-36 questionnaire at 3 and 12 months post-COVID-19 infection. The greatest differences were in the physical role (RF) and in the emotional role (RE). Conclusions: COVID-19 pneumonia causes a long-term deterioration in HRQoL compared to the general population. Over time, a trend toward improvement is detected in most domains of the SF-36.
Analysis of Amino Acids in the Roots of Tamarix ramosissima by Application of Exogenous Potassium (K+) under NaCl Stress
Authors: Chen Yahui , Zhang Shiyang , Du Shanfeng , Zhang Xiaomian , Jiang Jiang , Wang Guangyu
Published: August 2022

Soil salinization is one of the main environmental factors affecting plant growth worldwide. Tamarix ramosissima Ledeb. (T. ramosissima) is a halophyte representative that is widely grown in salinized soils. As an important nutrient element for plant growth, K+ plays an important role in improving the tolerance to salt stress, but the mechanism of reducing the damage caused by NaCl stress to T. ramosissima is less reported. Our results show that the proline content and the Log2 fold-change of proline’s relative quantification in the roots of T. ramosissima increased over time with the application of exogenous potassium (K+) for 48 h and 168 h under NaCl stress. Moreover, 13 amino-acid-related metabolic pathways were involved in the resistance of T. ramosissima to salt stress. Mainly, the aldehyde dehydrogenase family genes and tryptophan-synthase-related genes were found at 48 h and 168 h with exogenous potassium applied to the roots of T. ramosissima under NaCl stress, and they regulated their related metabolic accumulation in the arginine and proline metabolism pathways, increasing the effectiveness of inducing NaCl tolerance of T. ramosissima. It is noteworthy that alpha-ketobutyric was produced in the roots of T. ramosissima under NaCl stress for 48 h with the application of exogenous potassium, which is one of the most effective mechanisms for inducing salt tolerance in plants. Meanwhile, we found three DEGs regulating alpha-ketobutyric acid. This study provides a scientific theoretical basis for further understanding the molecular mechanism of K+ alleviating the salinity damage to T. ramosissima caused by NaCl.
Sex Differences in Adiposity and Cardiovascular Diseases
Authors: Li Haoyun , Konja Daniels , Wang Luyao , Wang Yu
Published: August 2022

Body fat distribution is a well-established predictor of adverse medical outcomes, independent of overall adiposity. Studying body fat distribution sheds insights into the causes of obesity and provides valuable information about the development of various comorbidities. Compared to total adiposity, body fat distribution is more closely associated with risks of cardiovascular diseases. The present review specifically focuses on the sexual dimorphism in body fat distribution, the biological clues, as well as the genetic traits that are distinct from overall obesity. Understanding the sex determinations on body fat distribution and adiposity will aid in the improvement of the prevention and treatment of cardiovascular diseases (CVD).
OsASR6 Enhances Salt Stress Tolerance in Rice
Authors: Zhang Qin , Liu Yuqing , Jiang Yingli , Li Aiqi , Cheng Beijiu , Wu Jiandong
Published: August 2022

High salinity seriously affects crop growth and yield. Abscisic acid-, stress-, and ripening-induced (ASR) proteins play an important role in plant responses to multiple abiotic stresses. In this study, we identified a new salt-induced ASR gene in rice (OsASR6) and functionally characterized its role in mediating salt tolerance. Transcript levels of OsASR6 were upregulated under salinity stress, H2O2 and abscisic acid (ABA) treatments. Nuclear and cytoplasmic localization of the OsASR6 protein were confirmed. Meanwhile, a transactivation activity assay in yeast demonstrated no self-activation ability. Furthermore, transgenic rice plants overexpressing OsASR6 showed enhanced salt and oxidative stress tolerance as a result of reductions in H2O2, malondialdehyde (MDA), Na/K and relative electrolyte leakage. In contrast, OsASR6 RNAi transgenic lines showed opposite results. A higher ABA content was also measured in the OsASR6 overexpressing lines compared with the control. Moreover, OsNCED1, a key enzyme of ABA biosynthesis, was found to interact with OsASR6. Collectively, these results suggest that OsASR6 serves primarily as a functional protein, enhancing tolerance to salt stress, representing a candidate gene for genetic manipulation of new salinity-resistant lines in rice.
Efficient Adsorption-Photocatalytic Removal of Tetracycline Hydrochloride over Octahedral MnS
Authors: Guo Jing , Liu Tingting , Peng Hao , Zheng Xiaogang
Published: August 2022

To disclose the effect of crystal plane on the adsorption-photocatalytic activity of MnS, octahedral MnS was prepared via the hydrothermal route to enhance the adsorption and photocatalytic efficiencies of tetracycline hydrochloride (TCH) in visible light region. The optimal MnS treated at 433 K for 16 h could remove 94.83% TCH solution of 260 mg L−1 within 180 min, and its adsorption-photocatalytic efficiency declined to 89.68% after five cycles. Its excellent adsorption-photocatalytic activity and durability were ascribed to the sufficient vacant sites of octahedral structure for TCH adsorption and the feasible band-gap structure for visible-light response. In addition, the band gap structure (1.37 eV) of MnS with a conduction band value of −0.58 eV and a valence band value of 0.79 eV was favorable for the generation of O2−, while unsuitable for the formation of OH. Hence, octahedral MnS was a potential material for the removal of antibiotics from wastewater.
Inflammatory Bowel Disease: A Review of Pre-Clinical Murine Models of Human Disease
Authors: Katsandegwaza Brunette , Horsnell William , Smith Katherine
Published: August 2022

Crohn’s disease (CD) and ulcerative colitis (UC) are both highly inflammatory diseases of the gastrointestinal tract, collectively known as inflammatory bowel disease (IBD). Although the cause of IBD is still unclear, several experimental IBD murine models have enabled researchers to make great inroads into understanding human IBD pathology. Here, we discuss the current pre-clinical experimental murine models for human IBD, including the chemical-induced trinitrobenzene sulfonic acid (TNBS) model, oxazolone and dextran sulphate sodium (DSS) models, the gene-deficient I-kappa-B kinase gamma (Iκκ-γ) and interleukin(IL)-10 models, and the CD4+ T-cell transfer model. We offer a comprehensive review of how these models have been used to dissect the etiopathogenesis of disease, alongside their limitations. Furthermore, the way in which this knowledge has led to the translation of experimental findings into novel clinical therapeutics is also discussed.
Combined Treatment of Bronchial Epithelial Calu-3 Cells with Peptide Nucleic Acids Targeting miR-145-5p and miR-101-3p: Synergistic Enhancement of the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Gene
Authors: Papi Chiara , Gasparello Jessica , Zurlo Matteo , Manicardi Alex , Corradini Roberto , Cabrini Giulio , Gambari Roberto , Finotti Alessia
Published: August 2022

The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene encodes for a chloride channel defective in Cystic Fibrosis (CF). Accordingly, upregulation of its expression might be relevant for the development of therapeutic protocols for CF. MicroRNAs are deeply involved in the CFTR regulation and their targeting with miRNA inhibitors (including those based on Peptide Nucleic Acids, PNAs)is associated with CFTR upregulation. Targeting of miR-145-5p, miR-101-3p, and miR-335-5p with antisense PNAs was found to be associated with CFTR upregulation. The main objective of this study was to verify whether combined treatments with the most active PNAs are associated with increased CFTR gene expression. The data obtained demonstrate that synergism of upregulation of CFTR production can be obtained by combined treatments of Calu-3 cells with antisense PNAs targeting CFTR-regulating microRNAs. In particular, highly effective combinations were found with PNAs targeting miR-145-5p and miR-101-3p. Content of mRNAs was analyzed by RT-qPCR, the CFTR production by Western blotting. Combined treatment with antagomiRNAs might lead to maximized upregulation of CFTR and should be considered in the development of protocols for CFTR activation in pathological conditions in which CFTR gene expression is lacking, such as Cystic Fibrosis.
Expression of MicroRNAs in Sepsis-Related Organ Dysfunction: A Systematic Review
Authors: Maiese Aniello , Scatena Andrea , Costantino Andrea , Chiti Enrica , Occhipinti Carla , La Russa Raffaele , Di Paolo Marco , Turillazzi Emanuela , Frati Paola , Fineschi Vittorio
Published: August 2022

Sepsis is a critical condition characterized by increased levels of pro-inflammatory cytokines and proliferating cells such as neutrophils and macrophages in response to microbial pathogens. Such processes lead to an abnormal inflammatory response and multi-organ failure. MicroRNAs (miRNA) are single-stranded non-coding RNAs with the function of gene regulation. This means that miRNAs are involved in multiple intracellular pathways and thus contribute to or inhibit inflammation. As a result, their variable expression in different tissues and organs may play a key role in regulating the pathophysiological events of sepsis. Thanks to this property, miRNAs may serve as potential diagnostic and prognostic biomarkers in such life-threatening events. In this narrative review, we collect the results of recent studies on the expression of miRNAs in heart, blood, lung, liver, brain, and kidney during sepsis and the molecular processes in which they are involved. In reviewing the literature, we find at least 122 miRNAs and signaling pathways involved in sepsis-related organ dysfunction. This may help clinicians to detect, prevent, and treat sepsis-related organ failures early, although further studies are needed to deepen the knowledge of their potential contribution.
The Omnipresence of DYRK1A in Human Diseases
Authors: Deboever Estelle , Fistrovich Alessandra , Hulme Christopher , Dunckley Travis
Published: August 2022

The increasing population will challenge healthcare, particularly because the worldwide population has never been older. Therapeutic solutions to age-related disease will be increasingly critical. Kinases are key regulators of human health and represent promising therapeutic targets for novel drug candidates. The dual-specificity tyrosine-regulated kinase (DYRKs) family is of particular interest and, among them, DYRK1A has been implicated ubiquitously in varied human diseases. Herein, we focus on the characteristics of DYRK1A, its regulation and functional role in different human diseases, which leads us to an overview of future research on this protein of promising therapeutic potential.
Innovation of Imine Metal Chelates as Corrosion Inhibitors at Different Media: A Collective Study
Authors: El-Lateef Hany M. Abd , El-Dabea Tarek , Khalaf Mai M. , Abu-Dief Ahmed M.
Published: August 2022

The corrosion inhibition of transition metal chelates derived from Schiff base ligands was tested for (mild, copper, stainless, aluminum and carbon) steel in various concentrations of (HCl, HNO3 and H2SO4) acidic medium at 25 °C through (weight loss, potentiodynamic polarization, polarization curves, electrochemical impedance spectroscopy (EIS) and open circuit potential measurements (OCP)) techniques. The studied compounds were identified with various spectral, analytical and physico-chemical techniques. It was observed that the investigated compounds had a significant inhibitory impact on the corrosion of diverse steels in the medium investigated. The analysis shows that increasing the dose of the studied complexes improves the corresponding inhibitory efficiency values. Negative results of Gibb’s free adsorption energy (ΔGads0) prove the suppression process’s spontaneous and physical adsorption, which contradicts the Langmuir adsorption isotherm. As a result of this insight, a novel bridge between nuclearity driven coordinated inorganic chemistry and materials, as well as corrosion control, has been built. This review provides an overview of the use of Schiff bases and associated transition metals as potential corrosion inhibitors, including the factors that influence their application.