bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential causal relationships between blood metabolites, inflammatory cytokines, and venous thromboembolism
Authors: Liu Qianying , Yang Fan , Kong Kangli , Lu Fangfang
Published: September 2024

Background

Venous thromboembolism (VTE) is the abnormal coagulation of blood in deep veins, which impairs venous return and includes deep vein thrombosis (DVT) and pulmonary embolism (PE). The incidence of VTE is increasing, leading to severe complications and sequelae. Despite the widespread application of multi-omics analyses in vascular disease research, identifying the specific links between various metabolic products, cytokines, and VTE, as well as their potential mediating roles, requires further validation due to confounding factors.

Methods

Summary statistics for 1,091 metabolites, 309 metabolite ratios (8,299 individuals), and 41 inflammatory cytokines (8,293 individuals) were obtained from the largest genome-wide association studies (GWAS). Summary statistics for VTE (21,021 cases, 391,160 controls), DVT (6,501 cases, 357,111 controls), and PE (10,046 cases, 401,128 controls) were derived from the FinnGen R10 dataset. We initially examined causal relationships using two-sample MR analysis, followed by Two-step Mendelian Randomization (TSMR) and Multivariable Mendelian Randomization (MVMR) to identify potential mediating mechanisms.

Results

We identified causal associations for 78 blood metabolites with VTE, 79 with DVT, and 81 with PE. Among all 41 inflammatory cytokines included, only platelet-derived growth factor BB (PDGF-BB) levels showed a causal relationship with increased risks of VTE, DVT, and PE. MVMR analysis revealed that the associations between glycocholate levels and VTE, DVT, and PE were mediated by PDGF-BB, accounting for 14.54% (p=2.84E-04), 17.10% (p=3.64E-05), and 10.44% (p=1.39E-02), respectively. Furthermore, the associations between dodecanedioate (C12:1-DC) levels and VTE and DVT were also mediated by PDGF-BB, accounting for 12.79% (p=6.10E-04) and 12.17% (p=2.13E-04), respectively.

Conclusion

This study reveals significant associations between specific blood metabolites and the risks of VTE, DVT, and PE, with some associations potentially mediated by PDGF-BB.
Integrating machine learning to advance epitope mapping
Authors: Grewal Simranjit , Hegde Nidhi , Yanow Stephanie K.
Published: September 2024

Identifying epitopes, or the segments of a protein that bind to antibodies, is critical for the development of a variety of immunotherapeutics and diagnostics. In vaccine design, the intent is to identify the minimal epitope of an antigen that can elicit an immune response and avoid off-target effects. For prognostics and diagnostics, the epitope-antibody interaction is exploited to measure antigens associated with disease outcomes. Experimental methods such as X-ray crystallography, cryo-electron microscopy, and peptide arrays are used widely to map epitopes but vary in accuracy, throughput, cost, and feasibility. By comparing machine learning epitope mapping tools, we discuss the importance of data selection, feature design, and algorithm choice in determining the specificity and prediction accuracy of an algorithm. This review discusses limitations of current methods and the potential for machine learning to deepen interpretation and increase feasibility of these methods. We also propose how machine learning can be employed to refine epitope prediction to address the apparent promiscuity of polyreactive antibodies and the challenge of defining conformational epitopes. We highlight the impact of machine learning on our current understanding of epitopes and its potential to guide the design of therapeutic interventions with more predictable outcomes.
Mechanistic studies of tumor-associated macrophage immunotherapy
Authors: Cao Jiandong , Liu Chengsen
Published: September 2024

Tumor-associated macrophages (TAMs) are present in the tumor microenvironment and can polarize into subtypes with different functions and characteristics in response to different stimuli, classifying them into anti-tumorigenic M1-type and pro-tumorigenic M2-type. The M1-type macrophages inhibit tumor growth through the release of pro-inflammatory cytokines, whereas the M2-type macrophages contribute to tumor progression through the promotion of tumor proliferation, angiogenesis and metastasis. Due to the duality of macrophage effects on tumors, TAMs have been a hot topic in tumor research. In this paper, the heterogeneity and plasticity of TAMs, the interactions between TAMs and other immune cells, and the effects of TAMs on tumors are reviewed, and the therapeutic strategies for TAMs are summarized and discussed. These therapeutic strategies encompass methods and approaches to inhibit the recruitment of TAMs, deplete TAMs, and modulate the polarization of TAMs. These studies help to deeply understand the mechanism of TAMs-tumor interaction and provide reference for combination therapy of tumors.
Advantages of the zebrafish tumor xenograft model: the evaluation of efficacy in cancer therapy and the application to the study of lncRNAs
Authors: Hu Chengwu , Sun Ling , Chen Jianqing , Lyu Zhengbing , Yuan Chen , Jiang Xiaofeng
Published: September 2024

In the current preclinical anti-tumor researches, there is a general lack of an in vivo model that can quickly and efficiently screen effective anti-tumor drugs. As a species that is 87% genetically similar to humans, zebrafish have been widely used to model human diseases, and they are considered an alternative economic model for studying cancer development, proliferation, and metastasis. The zebrafish tumor xenograft model has been effectively used for cancer drug development at all levels, including target validation, and high-throughput screening of long non-coding RNAs (lncRNAs) that may be involved in tumor regulation. In this review, we provide a comprehensive overview of zebrafish as an in vivo model for cancer cell growth, migration, anti-tumor immunotherapy, and anti-tumor drug screening. In addition, the regulatory mechanisms of some active lncRNAs have been identified to play a role in the pathogenesis of cancer, but it is still necessary to take advantage of the efficient zebrafish model to screen and learn more about the role of these molecules in tumor development and migration. Current anti-tumor therapies are limited by severe toxicity and multidrug resistance. There is an urgent need for the cost-effective and efficient in vivo research tools to improve our understanding and overcome these problems. This paper reviews the different purposes of anti-tumor research using zebrafish model. We discuss the use of zebrafish in cancer cell proliferation and metastasis, identifying signaling pathways, cancer drug discovery and treatment development, and toxicity studies. Finally, this review highlights the limitations of the field and future directions to effectively utilize zebrafish as a highly efficient model for cancer treatment development.
Clinical characteristics and visual outcomes of animal-induced ocular injuries: a prospective multicenter study in Iran
Authors: Mohammadi Mohammad , Attar Alireza , Mahmoudinezhad Golnoush , Shahesmaeilinejad Armita , Zhu Dagny , Fowler Brian , Farsi Yeganeh , Shirvani Mohammad , Gohari Mohsen
Published: September 2024

Background

Animal-induced ocular injuries represent an under-documented health problem that may pose significant visual complications. This study aimed to investigate the clinical characteristics and outcomes of ocular injuries caused by animals.

Methods

This multicenter prospective study enrolled patients with a history of animal-induced ocular injuries presenting to the ophthalmology departments of two tertiary hospitals over a one-year period. All participants underwent comprehensive assessments by ophthalmologists, and the following data were collected: demographic information, animal species involved, injury details, pre- and post-treatment visual data, management strategies, and follow-up outcomes.

Results

Seventy-two patients (62.5% male) were included in the study. Insects were the most common species, causing 37.5% of ocular injuries. The type of animal involved was significantly associated with injury patterns (p < 0.0001), visual impairments at presentation (p < 0.05), and need for surgery (p < 0.001). Insects predominantly caused periorbital soft tissue injuries; birds primarily affected the anterior segment; dogs and cats mainly led to adnexal injuries; and equines often involved both anterior and posterior segments. Equine-related injuries resulted in the most severe visual impairments, requiring surgical interventions in all cases. At the same time, the majority of cases involved with other animal species experienced no visual impairment. The number of patients without visual impairment increased from 46 cases (63.9%) at the time of presentation to 58 (80.6%) at discharge after treatment. At follow-up, eight patients (11.1%) experienced complications, including traumatic cataract (n = 6, 8.3%), endophthalmitis (n = 3, 4.2%), corneal scarring (n = 1, 1.4%), and retinal detachment (n = 1, 1.4%). Rooster pecking was the leading cause of complications, including endophthalmitis and traumatic cataract.

Conclusion

This study revealed that depending on the type of animal causing the injury, animal-induced ocular injuries present with diverse characteristics, requiring appropriate treatment approaches and potentially resulting in different outcomes. These findings may promote public awareness and improve preventive strategies and clinical guidelines development.
The use of plasma exchange with albumin replacement in the management of Alzheimer’s disease: a scoping review
Authors: Cantero-Fortiz Yahveth , Boada Mercè
Published: September 2024

Introduction

AD is a progressive neurodegenerative disorder causing significant cognitive decline and impaired daily functioning. Current treatments offer only modest relief, and many amyloid-targeting therapies have failed, prompting exploration of alternative approaches such as PE with albumin replacement.

Objectives

This scoping review systematically maps the literature on PE with albumin replacement in AD management, focusing on outcomes, methodologies, and reported benefits and risks.

Methods

A comprehensive search in PubMed, supplemented by reference scanning and hand-searching, identified studies involving PE with albumin replacement in AD patients. Data charting and critical appraisal were conducted using standardized tools.

Results

Seven primary studies from the AMBAR (Alzheimer Management by Albumin Replacement) trial met the inclusion criteria, consistently reporting improvements in cognitive function, positive neuroimaging results, and favorable neuropsychiatric outcomes. For instance, one study found a significant slowing of cognitive decline (p < 0.05) among patients receiving PE with albumin replacement. Another study showed better preservation of hippocampal volume and improved brain perfusion metrics in the treatment group (p < 0.05). The intervention was generally well-tolerated with manageable side effects.

Conclusion

PE with albumin replacement is a promising therapeutic approach for AD, warranting further investigation to confirm its efficacy and safety across broader settings.

Scoping review registration

https://osf.io/v6dez/?view_only=1cd9637e7e0347d39713bf19aac0dfe8.
The role of isothiocyanate-rich plants and supplements in neuropsychiatric disorders: a review and update
Authors: Ramakrishnan Monica , Fahey Jed W. , Zimmerman Andrew W. , Zhou Xinyi , Panjwani Anita A.
Published: September 2024

Neuroinflammation in response to environmental stressors is an important common pathway in a number of neurological and psychiatric disorders. Responses to immune-mediated stress can lead to epigenetic changes and the development of neuropsychiatric disorders. Isothiocyanates (ITC) have shown promise in combating oxidative stress and inflammation in the nervous system as well as organ systems. While sulforaphane from broccoli is the most widely studied ITC for biomedical applications, ITC and their precursor glucosinolates are found in many species of cruciferous and other vegetables including moringa. In this review, we examine both clinical and pre-clinical studies of ITC on the amelioration of neuropsychiatric disorders (neurodevelopmental, neurodegenerative, and other) from 2018 to the present, including documentation of protocols for several ongoing clinical studies. During this time, there have been 16 clinical studies (9 randomized controlled trials), most of which reported on the effect of sulforaphane on autism spectrum disorder and schizophrenia. We also review over 80 preclinical studies examining ITC treatment of brain-related dysfunctions and disorders. The evidence to date reveals ITC have great potential for treating these conditions with minimal toxicity. The authors call for well-designed clinical trials to further the translation of these potent phytochemicals into therapeutic practice.
Case report: Fatal hemoptysis after effective treatment with tislelizumab and anlotinib in pulmonary sarcomatoid carcinoma
Authors: Pu Chen-Wei , Ma Yong-Fen , Peng Jing-Jing , Wang Zhen-Zhen
Published: September 2024

Pulmonary sarcomatoid carcinoma (PSC), a rare non-small cell lung cancer (NSCLC) subtype, poses diagnostic and treatment difficulties. Current research explores targeted therapies and immunotherapy to improve patient outcomes. This case report details a male patient diagnosed with PSC via pathology. Tests revealed high levels of PD-L1, a marker suggesting potential benefit from immune checkpoint inhibitors. However, despite bronchoscopic intervention, his advanced stage IIIB cancer (cT3N2bM0) progressed quickly, with progression-free survival (PFS) under 3 months. Following progression, the patient received tislelizumab (anti-PD-1 antibody) and anlotinib (an anti-angiogenic drug) as second-line therapy. This combination showed promise, achieving near-partial remission after the first cycle. Subsequent scans documented continued tumor shrinkage until the patient experienced fatal hemoptysis. This case highlights the potential benefits of combining tislelizumab with anlotinib for PSC. However, it also represents the first reported case of fatal hemoptysis with this specific treatment regimen. This finding emphasizes the need for increased awareness of this potential complication, especially in patients with centrally located PSC treated with anti-angiogenic agents like anlotinib.
Predicting the risk of colorectal cancer among diabetes patients using a random survival forest-guided approach
Authors: Yau Sarah Tsz Yui , Hung Chi Tim , Leung Eman Yee Man , Chong Ka Chun , Lee Albert , Yeoh Eng Kiong
Published: September 2024

Background

Colorectal cancer (CRC) is the third most frequently diagnosed cancer worldwide. Diabetes and CRC share many overlapping lifestyle risk factors such as obesity, heavy alcohol use, and diet. This study aims to develop a risk scoring system for CRC prediction among diabetes patients using routine medical records.

Methods

A retrospective cohort study was conducted using electronic health records of Hong Kong. Patients who received diabetes care in public general outpatient clinics between 2010 and 2019 and had no cancer history were identified, and followed up until December 2019. The outcome was diagnosis of CRC during follow-up. For model building, predictors were first selected using random survival forest, and weights were subsequently assigned to selected predictors using Cox regression.

Results

Of the 386,325 patients identified, 4,199 patients developed CRC during a median follow-up of 6.2 years. The overall incidence rate of CRC was 1.93 per 1000 person-years. In the final scoring system, age, waist-to-hip ratio, and serum creatinine were included as predictors. The C-index on test set was 0.651 (95%CI: 0.631-0.669). Elevated serum creatinine (≥127 µmol/L) could be a potential important predictor of increased CRC risk.

Conclusion

While obesity is a well-known risk factor for CRC, renal dysfunction could be potentially linked to an elevated risk of CRC among diabetes patients. Further studies are warranted to explore whether renal function could be a potential parameter to guide screening recommendation for diabetes patients.
Precision measurement of rehabilitation interventions—a secondary analysis of motor error in a clinical trial with young children with cerebral palsy
Authors: Skorup Julie C. , Pierce Samuel R. , Paremski Athylia C. , Alcott Morgan , Prosser Laura A.
Published: September 2024

Introduction

The delivery of precision medicine in rehabilitation will require not only precise measurement of participant response, but also precise measurement of the “ingredients” of intervention and their dose. As an example, we report the measurement of motor error in two treatment groups from a randomized controlled trial in toddlers (mean age 26.3 months) with cerebral palsy (CP). Our objective was to measure the type and amount of motor error during physical therapy sessions in young children with CP.

Methods

Participants were stratified by motor function and age and randomly allocated to “conventional” physical therapy that generally prevented falls or to an intervention that encouraged error experience by not preventing falls (experimental group). Baseline motor and cognitive function were measured using the Gross Motor Function Measure-66 (GMFM-66) and Bayley 3 cognitive subscale (B3-C) prior to randomization. Randomly selected video recorded therapy sessions were manually coded to identify losses of balance defined as falls (child contacted floor), rescues (therapist prevented fall) or saves (child recovered their balance independently).

Results

Average number of losses of balance per session were higher in the experimental group than the conventional group due to significantly greater falls. Saves were infrequent in both groups but were also significantly higher in the experimental group. Average number of rescues did not differ between groups. In the experimental group, greater frequency of falls was significantly related to GMFM-66. In both groups, greater frequency of saves was related to GMFM-66. Neither total losses of balance per session nor rescues were related to GMFM-66 in either group. There were no significant relationships between losses of balance and baseline cognition in either group, except greater frequency of saves was related to higher cognitive ability in the experimental group.

Discussion

Our observations suggest that motor error experience is lower in toddlers with CP compared to peers with typical development but can be manipulated to higher doses of error during therapy sessions. Future work should investigate the relationship between type and amount of error experience and rehabilitation outcomes, as well as other “ingredients” of rehabilitation therapy. Tools to automate the precise measurement of intervention content are necessary for broad scale implementation.
An insight into pharmacokinetics and dose optimization of antimicrobials agents in elderly patients
Authors: Fu Guanshuang , Sun Weijia , Tan Zhaoyi , Liang Beibei , Cai Yun
Published: September 2024

The global elderly population is on the rise, and infections tend to have a higher mortality rate among older individuals. Aging is associated with the progressive impairment of multi-organ function, which can impact the pharmacokinetics of antimicrobials, potentially leading to the failure of anti-infective therapy. With the increasing life expectancy, a significant growth in the elderly demographic, and the escalating costs of healthcare, gaining a thorough understanding of pharmacokinetic changes in the elderly holds crucial clinical significance. This review compiles findings from published studies, offering a comprehensive overview of the pharmacokinetics of various antimicrobials in both adults and the elderly. Furthermore, it delves into advancements in pharmacokinetic methods specific to the elderly population.
A practical assessment protocol for clinically relevant P-glycoprotein-mediated drug-drug interactions
Authors: Bogaard Leonie , Tsoi Kayan , van de Steeg Bas , Brandon Esther F. A. , Geers Lisanne , van Herwaarden Margreet , Jansman Frank , Maas Dominique , Monster-Simons Margje , Ong David S. Y. , Borgsteede Sander D.
Published: September 2024

Background

Drug-drug interactions (DDIs) may influence the effectiveness and safety of medication treatment, which may require additional monitoring, dose adjustment or avoidance of certain drugs. DDIs involving P-glycoprotein (P-gp) affect many drugs, but current official product information is often insufficient to guide the management of these DDIs in clinical practice. The aim of this paper is to describe a protocol to assess DDIs involving P-gp and to develop and implement practice recommendations for clinically relevant P-gp-mediated DDIs that affect clinical outcomes through changes in systemic drug exposure.

Methods

A combined literature review and expert opinion approach will be used according to the following seven steps: set up an expert panel (step 1), establish core concepts and definitions (step 2), select potential P-gp-modulators (i.e., P-gp-inducers and -inhibitors) and P-gp-substrates to be evaluated (step 3), select and extract evidence-based data, and present findings in standardized assessment reports (step 4), discuss and adopt classifications and practice recommendations with the expert panel (step 5), publish and integrate information and alerts in clinical decision support systems (CDSS) (step 6), (re)assessments of DDIs and potential new DDIs when new information is available or when initiated by healthcare providers (step 7).

Anticipated results

The expert panel will classify potential P-gp-modulators and -substrates as clinically relevant P-gp-inducer, -inhibitor and/or -substrate and draw conclusions about which combinations of classified modulators and substrates will lead to clinically relevant DDIs. This may include the extrapolation of conclusions for DDIs where limited or no data are available, based on the pharmacological characteristics of these drugs. For (potential) DDIs that are considered to be clinically relevant, practice recommendations will be developed.

Discussion

This protocol describes a standardized, evidence- and expert opinion-based assessment of P-gp-mediated DDIs that affect clinical outcomes. This approach will generate alerts with practice recommendations for clinically relevant DDIs and transparent rationales for DDIs that are considered to be irrelevant. These recommendations will improve individual patient care by supporting healthcare professionals to make consistent decisions on how to manage P-gp mediated DDIs.
Gaucher disease in Brazil: a comprehensive 16 year retrospective study on survival, cost, and treatment insights
Authors: Borin Marcus Carvalho , Alvares-Teodoro Juliana , Acurcio Francisco Assis , Guerra Augusto Afonso
Published: September 2024

Background

Gaucher’s disease (GD), a lysosomal storage disorder, poses significant treatment challenges. This 23-year study assesses survival rates and treatment efficacy in Brazilian GD patients, integrating data from a 16-year cohort (2000–2015) and the TABNET/DATASUS medicines distribution data (1999–2022).

Objective

To investigate the survival of GD patients in Brazil, identifying key risk factors and evaluating the impact of treatments funded by the Brazilian National Health System (SUS).

Methodology

A 16-year retrospective cohort study was conducted using the National Database of SUS. Patients diagnosed with GD and treated with Enzyme Replacement Therapy (ERT) or Substrate Synthesis Inhibition (SSI) from 2000 to 2015 were included. Survival analysis was performed using Kaplan-Meier method and Cox proportional hazards model. The data from TABNET/DATASUS system from 1999 to 2022 was used to assess the trend in drug distribution beyond the main cohort.

Results

The study included 1,234 patients. Survival rates at 5 and 10 years were 93.2% and 88.5%, respectively, with age and comorbidities like diabetes, cardiovascular diseases, and Parkinson’s disease significantly affecting survival. Patients who received doses lower than DDD (n = 880) demonstrated a survival probability of 91.8%. In contrast, those with doses equal to the DDD (n = 15) showed a 100% survival probability, as no events were observed in this group. The greater than DDD group (n = 339) exhibited a survival probability of 81%. A log-rank test indicated a borderline statistical significance (p = 0.058) in the survival distributions among the different DDD adherence, with the lower dose group showing a favorable trend.

Conclusion

This study provides insights into the survival rates and associated risk factors for GD patients in Brazil, contributing to the global understanding of GD and its management. While we acknowledge the inherent limitations of relying largely on electronic medical records and categorical codes, our findings underscore the need for early diagnosis, timely initiation of treatment, effective management of comorbidities, and personalized dosing strategies to improve patient outcomes. Future studies should aim to incorporate clinical verification of electronic data to further enhance the reliability and applicability of these findings.
A survey in natural olive resources exposed to high inoculum pressure indicates the presence of traits of resistance to Xylella fastidiosa in Leccino offspring
Authors: La Notte Pierfederico , Saponari Maria , Mousavi Soraya , Mariotti Roberto , Abou Kubaa Raied , Nikbakht Roya , Melcarne Giovanni , Specchia Francesco , Altamura Giuseppe , Ligorio Angela , Boscia Donato , Surano Antony , Saldarelli Pasquale , Giampetruzzi Annalisa
Published: September 2024

Introduction

The epidemic spread of the harmful bacterium Xylella fastidiosa causing the “olive quick decline syndrome”, decimating olive trees in southern Italy, in the region of Apulia, prompted investigations to search for olive genotypes harbouring traits of resistance.

Methods

A prospecting survey was carried out to identify, in the heavily infected area of Apulia, olive genotypes bearing resistance. Given the limited genetic diversity in the commercial olive groves with few cultivars widely cultivated, surveys targeted predominantly spontaneous olive genotypes in natural and uncultivated areas. Trees, selected for the absence of symptoms, were subjected to diagnostic tests and parentage analysis to disclose their genetic background. Transcriptomic analyses were also employed to decipher the molecular pathways in resistant genotypes. Artificial inoculations were carried out to confirm the resistant phenotypes of four open-pollinated seedlings of the cultivar Leccino.

Results

Among the 171 olive collected genotypes, 139 had unique simple sequence repeat (SSR) profiles, with the cultivars Leccino, Cellina di Nardò, and Ogliarola salentina being the most frequent candidate parents. Among the Leccino progeny (n. 61), 67% showed a highly resistant (HR), resistant (R), or tolerant (T) phenotype to infection by X. fastidiosa. The occurrence of such phenotypes among those deriving from Cellina di Nardò and Ogliarola salentina was 32% and 49%, respectively. Analyses of the transcriptomic profiles of three Leccino-bearing genotypes, naturally infected and not showing symptoms, unravelled that a total of 17,227, 13,031, and 4,513 genes were found altered in the expression, including genes involved in photosynthesis, cell wall, or primary and secondary metabolism.

Discussion

Indeed, transcriptomic analyses showed that one of these genotypes (S105) was more resilient to changes induced by the natural bacterial infection than the remaining two (S215 and S234). This study consolidates the evidence on the presence and heritage of resistance traits associated with the cv. Leccino. Moreover, valuable insights were gathered when analysing their transcriptomic profiles, i.e., genes involved in mechanisms of response to the bacterium, which can be used in functional genetic approaches to introduce resistance in susceptible cultivars and initiate strategies in olive-breeding programs through marker-assisted selection.
Evaluation of the growth, enzymatic activity, electrolyte leakage, and phytoremediation efficiency of Conocarpus erectus under cadmium and lead stress
Authors: El-Mahrouk El-Sayed Mohamed , Eldawansy Shereen Mostafa , El-Tarawy Ahmed Mohamed , Ebrahim Hayam Mohamed Aly , Eisa Eman Abdelhakim , Tilly-Mándy Andrea , Honfi Péter
Published: September 2024

Contamination of agricultural soil by heavy metals poses a significant threat to soil quality and
crop yields. Using plants as a natural remediation approach attracts researchers’ attention around the world. A 16-month pot experiment was conducted using Conocarpus erectus in a randomized complete block design. The growth, enzymatic activity, electrolyte leakage, and remediation potential were estimated under Cd nitrate]40 low (L), 60 medium (M), 80 high (H) mg/kg soil [and Pb nitrate]400 (L), 700 (M), 1,000 (H) mg/kg soil [applied individually and in combination. Conocarpus erectus demonstrated a good tolerance (over 70%) against lower and medium cadmium (Cd) and lead (Pb) levels and a medium resistance against high Cd and Pb levels, with a survival rate of 100% under all the treatments used. The most negative treatment on the growth traits and tolerance of C. erectus was (H) Cd and (H) Pb, which reduced plant height; chlorophyll index; dry weights of the leaves, stems, and roots; root length; and tolerance index of biomass and roots by 25.87%, 48.97%, 50.56%, 47.25%, 58.67%, 50.18%, 51.00%, and 50% in comparison to the respective control, consecutively. Relative to the control, all Cd and Pb applications increased polyphenol oxidase (PPO), peroxidase (POD), and catalase (CAT) activities, and the increment was parallel up to medium Cd and Pb levels and then decreased with their high levels but still higher than the control. Electrolyte leakage (EL) was upheaved by raising the levels of Cd and Pb, and it reached the maximum (52.79%) at the (H) Cd (H) Pb treatment. Cd and Pb in the leaves, stems, and roots were boosted by raising their levels in the treatments. Conocarpus erectus is considered a phytoextractor for the Cd levels used because the bioconcentration factor of the stem (BCFs) and the translocation factor (TF) of Cd were >1, and it is a suitable plant for Pb phytoextraction at (L) Pb, (M) Pb, and (M) Cd (M) Pb levels because its Pb BCFs and bioconcentration factor of the root (BCFr) were <1 and its Pb TF was >1. On the other hand, C. erectus is considered a phytostabilizator for Pb at (H) Pb, (L) Cd, (L) Pb, and (H) Cd (H) Pb levels because its Pb BCFs, BCFr, and TF were <1.
The German Communities That Care Youth Survey: dimensionality and validity of risk factors
Authors: Reder Maren , Runge Ronja A. , Schlüter Helge , Soellner Renate
Published: September 2024

Background

Communities That Care (CTC) is an evidence-based community change strategy for supporting healthy youth development. One of its key elements is the development of a community profile to identify and prioritise risk factors for health and behavioural problems in adolescents based on the CTC Youth Survey. The strategy was originally developed and evaluated in the United States. An adapted version of the survey has been used in Germany since its first implementation in 2008. However, the dimensionality and validity of the adapted risk factor scales have not yet been evaluated. Therefore, this study aimed to confirm the assumed unidimensional structure and to establish the concurrent criterion validity of each risk factor.

Methods

A sample of 1,911 adolescents attending grades six to eleven in Lower-Saxony, Germany, was used to evaluate 23 risk factor scales of the German CTC Youth Survey covering the domains peer/individual, family, school, and community. Confirmatory factor analysis was used to assess the dimensional structure of all risk factors with sufficient item numbers. Goodness of fit was determined using CFI, SRMR, and RMSEA. Latent regression analysis tested the concurrent criterion validity of all unidimensional risk factors. For this, violent and delinquent behaviour, substance use, and depressive symptomatology were regressed on each risk factor.

Results

All evaluable risk factors demonstrated acceptable to good model fit regarding unidimensionality and predicted violent and delinquent behaviour, and substance use better than depressive symptomatology. Regarding the peer/individual risk factors, there are particularly high correlations with violent and delinquent behaviour, and substance use. In contrast, two risk factors were not correlated with substance use, whilst two other risk factors were not correlated with depressive symptomatology.

Conclusion

Overall, the results indicate that most risk factors demonstrate unidimensionality and are valid in terms of concurrent criterion validity. Although some risk factors were not correlated with every outcome, they still predicted most outcomes, suggesting that the CTC Youth Survey is a viable tool for communities to assess their community risk profile. Risk factors that did not demonstrate unidimensionality or concurrent criterion validity should be monitored in future surveys and interpreted with caution until further evidence is available.
Enhancing resistant starch fraction in modified elephant foot yam starch: optimizing preparation conditions and exploring tribological properties
Authors: Barua Sreejani , Srivastav Prem Prakash , Vilgis Thomas A.
Published: September 2024

Introduction

Elephant foot yam starch is highly susceptible to enzymatic digestion. To address this, heat-moisture treatment (HMT) has been used to modify its digestive properties and functionalities. However, when using modified starch in various food compositions, it is ultimately necessary to cook it in water-rich environments. The present work thus offers a systematic understanding of the heating and cooking procedures for modified starch, with a view to maintaining its structural and digestive properties.

Methods

Experimental investigations employing X-ray diffraction, scanning electron microscopy, and enzymatic digestion have been utilized to gain insights into the characteristics of cooked starches. The interactions between amylose and amylopectin in native starch are more susceptible to disruption during cooking, which results in changes in texture and a reduction in resistant starch (RS) retention. Additionally, these preparations have been investigated by tribology to test structure and and friction.

Results and discussion

The application of HMT has been observed to induce changes that stabilize the interactions between amylose and amylopectin, thereby ensuring better maintenance of granular integrity and higher RS retention under specific cooking conditions. The findings indicate that following a 2-min cooking period at 100°C, the modified elephant foot yam starch (EFYS) demonstrated enhanced granular stability, which was subsequently compromised after 10 min of cooking. Additionally, when modified elephant foot yam starch (EFYS) was heated at a temperature of 86°C for 2 and 6 min, it demonstrated superior preservation of resistant starch (RS) in comparison to a cooking time of 10 min. Moreover, the HMT starch exhibited the highest RS retention when cooked at 100°C for 2 and 6 min at approximately 39.12% and 20.23%, respectively. However, this retention decreased significantly to 1.08% after 10 min of cooking. Furthermore, the insights from tribology, when combined with the proposed naive models, enhance the understanding of how preparation conditions affect the functionalities of starches, which is crucial for designing food products with desired textural and nutritional qualities, especially in water-rich environments, where maintaining structural integrity and RS content is important.
How free trade pilot zones enhance agricultural green total factor productivity? Theory and evidence from China
Authors: Zeng Huasheng , Cheng Long , Ma Guoqun , Luo Yuxi
Published: September 2024

Introduction

Given the dual constraints of limited resources and environmental concerns, achieving green development in agriculture helps to enhance national food security, resource security, and ecological security.

Methods

Utilizing panel data sourced from 30 provinces within China, spanning the years from 2001 to 2021, this paper employs the super-efficiency Slack-Based Measure (SBM) model and the Global Malmquist-Luenberger (GML) index to measure agricultural green total factor productivity (AGTFP). It utilizes methods such as the stepwise Differences in Differences (DID), mediation effect models, and moderating effect models to systematically explore the impact of the implementation of Free Trade Pilot Zones (FTPZs) on AGTFP and its mechanisms of action.

Results

The study finds: (1) Establishing FTPZs significantly boosts AGTFP, indicating a profound positive influence; (2) The promotional effect of FTPZs on AGTFP is stronger in the eastern and central regions, as well as in major grain-producing areas; (3) FTPZs may enhance AGTFP by promoting the effect of agricultural structure upgrading, i.e., a transmission mechanism exists from FTPZs to the agricultural structure upgrading effect to AGTFP; (4) The agricultural scale effect plays a positive moderating role in the impact of FTPZs on AGTFP.

Discussion

It is advisable to optimize agricultural measures related to FTPZs, promote agricultural structural upgrading and scale enhancement, adapt strategies to local conditions to advance green agricultural development, and thereby promote the coordinated development of FTPZs and AGTFP.
Intraoperative marking of pulmonary nodules in a hybrid operating room: electromagnetic navigation bronchoscopy versus percutaneous marking
Authors: Gómez-Hernández María Teresa , Rivas Duarte Cristina E. , Fernández García-Hierro José María , Fuentes Marta G. , Colmenares Oscar , Forcada Barreda Clara , Gómez Valle Francisco , Jiménez García Irene , Jiménez Marcelo F.
Published: September 2024

Background

Intraoperative identification of subsolid or small pulmonary nodules during minimally invasive procedures is challenging. Recent localization techniques show varying success and complications. Hybrid operating rooms (HORs), equipped with radiological tools, facilitate intraoperative imaging. This study compares the accuracy and safety of marking pulmonary nodules using electromagnetic navigation bronchoscopy (ENB) combined with Cone Beam Computed Tomography (CBCT) vs. CBCT-guided percutaneous marking (PM).

Methods

This retrospective cohort study included patients with pulmonary nodules scheduled for minimally invasive resection in a HOR. Marking techniques included ENB assisted by CBCT and PM guided by CBCT. The study compared the success rate, procedure time, intraoperative complications and radiation dose of both techniques.

Results

A total of 104 patients with 114 nodules were included (October 2021—July 2024). Thirty nodules were marked using ENB, and 84 with PM. One case used both techniques due to ENB failure. No differences among groups were found in nodule characteristics. Success rates were similar (93.3% in ENB group vs. 91.7% in PM group, p = 1). Marking took significantly longer time in the ENB group (median 40 min) compared to PM group (25 min, p = 0.007). Five (6%) patients in the PM group experienced intraoperative complications compared to none in the ENB (p = 0.323). Radiation dose was significantly higher in the ENB group (p = 0.002).

Conclusions

ENB assisted by CBCT is a safe and effective technique, with success rates comparable to CBCT-guided PM, though it may result in longer procedural times and higher radiation doses.
Case report: Antibodies to myelin basic protein in a podenco-crossbreed dog with seizures
Authors: Deutschland M. , Boettcher I. , Höltje M. , Prüss H.
Published: September 2024

A 6-year-old female spayed Podenco-crossbreed dog was presented with an unusual type of focal impaired awareness seizures, including sensory ataxia and postictal rest. Magnetic resonance imaging examination revealed pre- and post-contrast agent T1-weighted bilateral symmetric hyperintensities in the lentiform nuclei and globus pallidus. Repeated cerebrospinal fluid sampling showed lymphocytic pleocytosis. Cerebrospinal fluid immunoglobulin G autoantibodies to myelin basic protein (MBP) were detected by immunofluorescence examination with strong binding to myelinated fiber tracts. The absence of binding to MBP-depleted mouse brains confirmed MBP as an antigenic target. Although the patient had minor seizure episodes every 2 months, and the owners avoided seizure triggers, they refused medical treatment before presenting to the veterinarian. To the best of our knowledge, this is the first description of MBP autoantibody-positive encephalitis in a dog.
RNA-Seq based selection signature analysis for identifying genomic footprints associated with the fat-tail phenotype in sheep
Authors: Abbasabadi Hossein , Bakhtiarizadeh Mohammad Reza , Moradi Mohammad Hossein , McEwan John C.
Published: September 2024

Understanding the genetic background behind fat-tail development in sheep can be useful to develop breeding programs for genetic improvement, while the genetic basis of fat-tail formation is still not well understood. Here, to identify genomic regions influencing fat-tail size in sheep, a comprehensive selection signature identification analysis was performed through comparison of fat- and thin-tailed sheep breeds. Furthermore, to gain the first insights into the potential use of RNA-Seq for selection signature identification analysis, SNP calling was performed using RNA-Seq datasets. In total, 45 RNA-Seq samples from seven cohort studies were analyzed, and the FST method was used to detect selection signatures. Our findings indicated that RNA-Seq could be of potential utility for selection signature identification analysis. In total, 877 SNPs related to 103 genes were found to be under selection in 92 genomic regions. Functional annotation analysis reinforced the hypothesis that genes involved in fatty acid oxidation May modulate fat accumulation in the tail of sheep and highlighted the potential regulatory role of angiogenesis process in the fat deposition. In agreement with most previous studies, our results re-emphasize that the BMP2 gene is targeted by selection during sheep evolution. Further gene annotation analysis of the regions targeted by the sheep evolution process revealed that a large number of genes included in these regions are directly associated with fat metabolism, including those previously reported as candidates involved in sheep fat-tail morphology, such as NID2, IKBKG, RGMA, IGFBP7, UBR5, VEGFD and WLS. Moreover, a number of genes, including BDH2, ECHS1, AUH, ERBIN and CYP4V2 were of particular interest because they are well-known fat metabolism-associated genes and are considered novel candidates involved in fat-tail size. Consistent with the selection signature identification analysis, principal component analysis clustered the samples into two completely separate groups according to fat- and thin-tailed breeds. Our results provide novel insights into the genomic basis of phenotypic diversity related to the fat-tail of sheep breeds and can be used to determine directions for improving breeding strategies in the future.
A Review of Recent Techniques for Human Activity Recognition: Multimodality, Reinforcement Learning, and Language Models
Authors: Oleh Ugonna , Obermaisser Roman , Ahammed Abu Shad
Published: September 2024

Human Activity Recognition (HAR) is a rapidly evolving field with the potential to revolutionise how we monitor and understand human behaviour. This survey paper provides a comprehensive overview of the state-of-the-art in HAR, specifically focusing on recent techniques such as multimodal techniques, Deep Reinforcement Learning and large language models. It explores the diverse range of human activities and the sensor technologies employed for data collection. It then reviews novel algorithms used for Human Activity Recognition with emphasis on multimodality, Deep Reinforcement Learning and large language models. It gives an overview of multimodal datasets with physiological data. It also delves into the applications of HAR in healthcare. Additionally, the survey discusses the challenges and future directions in this exciting field, highlighting the need for continued research and development to fully realise the potential of HAR in various real-world applications.
Impact of Organisational Climate on Employee Well-Being and Healthy Relationships at Work: A Case of Social Service Centres
Authors: Janiukštis Andrius , Kovaitė Kristina , Butvilas Tomas , Šūmakaris Paulius
Published: September 2024

Multiple studies highlight the crucial role of management of a positive organisational climate, which in turn contributes to employee well-being and healthy workplace relationships and mitigates the occurrence of negative behaviour, including harassment at the workplace. Furthermore, contemporary scholars emphasise the importance of open and transparent communication channels in reducing workplace tensions and improving employee mental health. However, the interrelationship between organisational climate, open communication, employee well-being, and negative behaviours in the workplace is a complex phenomenon. Despite the relevance and interest in organisational climate on employee well-being, limited efforts have been devoted to the area of relationships and causality, and straightforward answers about the objective links between these phenomena and their general nature remain complex. Therefore, this study aims to strengthen the understanding of the impact of the organisational climate on employee well-being and negative behaviours in the workplace. This is the first study that applied causality to the investigated problem to identify statistical relationships between the variables. This study was conducted in Lithuanian social service centres and schools, focussing on professionals with a high psychological risk at work due to their nature. This study offers valuable contributions and provides a comprehensive and profound insight into the management field, focussing on the impact of the organisational climate. The values of harassment in the workplace are explained by the values of organisational climate and employee well-being. This study demonstrates that improving the organisational climate contributes to employee well-being and healthy relationships and reduces the prevalence of negative behaviours in the workplace. This study introduces the academic and practical implications and suggests the pathways for further research.
Portuguese Textiles and Apparel Industry: Assessing the Effect of International Trade on Employment and Green Employment
Authors: Ribeiro Vitor Miguel
Published: September 2024

This study examines the impact of international trade activities on employment in the Portuguese textiles and apparel industry from 2010 to 2017. It finds evidence that imports and exports have a persistent, negative, and significant effect on overall job creation, with this impact intensifying over the long-run. Additionally, the increasing elasticity of substitution between imports and exports indicates that private companies of this industry have benefited from a win–win situation characterised by higher production volumes and lower marginal costs. By applying an unsupervised machine-learning method, followed by a discrete choice analysis to infer the firm-level propensity to possess green capital, we identify a phenomenon termed the green international trade paradox. This study also reveals that international trade activities positively influence green job creation in firms lacking green capital if and only if these players are engaged in international markets while negatively affecting firms already endowed with green technologies. As such, empirical results suggest that the export-oriented economic model followed over the last decade by the Portuguese textiles and apparel industry has not necessarily generated new domestic employment opportunities but has significantly altered the magnitude and profile of skill requirements that employers seek to identify in new workforce hires.