bluuebunny/crossref_metadata_2025 huggingface dataset:
Human Cytotoxicity, Hemolytic Activity, Anti-Inflammatory Activity and Aqueous Solubility of Ibuprofen-Based Ionic Liquids
Authors: Bastos Joana C. , Vieira Nicole S. M. , Gaspar Maria Manuela , Pereiro Ana B. , Araújo João M. M.
Published: August 2022

Ionic liquids (ILs) are a potential solution to the general problem of low solubility, polymorphism and low bioavailability of active pharmaceutical ingredients (APIs). In this work, we report on the synthesis of three pharmaceutically active ILs (API-ILs) based on ibuprofen, one of the most commonly available over-the-counter nonsteroidal anti-inflammatory drugs (NSAIDs), with imidazolium cations ([C2C1Im][Ibu] and [C2(OH)C1Im][Ibu]) and a cholinium cation ([N1112(OH)][Ibu]). An upgrade to the aqueous solubility (water and biological simulated fluids) for the ibuprofen-based ILs relative to the ibuprofen’s neutral and salt form (sodium ibuprofen) was verified. The cytotoxic profiles of the synthesized API-ILs were characterized using two human cells lines, Caco-2 colon carcinoma cells and HepG-2 hepatocellular carcinoma cells, up to ibuprofen’s maximum plasma concentration (Cmax) without impairing their cytotoxicity response. Additionally, the EC50 in the Caco-2 cell line revealed similar results for both parent APIs and API-ILs. The biocompatibility of the ibuprofen-based ILs was also evaluated through a hemolytic activity assay, and the results showed that all the ILs were hemocompatible at concentrations higher than the ibuprofen Cmax. Moreover, the anti-inflammatory properties of the API-ILs were assessed through the inhibition of bovine serum albumin (BSA) denaturation and inhibition of cyclooxygenases (COX-1 and COX-2). The results showed that [C2C1Im][Ibu], [C2(OH)C1Im][Ibu] and [N1112(OH)][Ibu] maintained their anti-inflammatory response to ibuprofen, with improved selectivity towards COX-2, allowing the development of safer NSAIDs and the recognition of new avenues for selective COX-2 inhibitors in cancer chemotherapy and neurological diseases such as Alzheimer’s and Parkinson’s.
Symmetry Reductions, Cte Method and Interaction Solutions for Sharma-Tasso-Olver-Burgers Equation
Authors: Yu Jun , Ren Bo , Wang Wan-Li
Published: August 2022

In this paper, the Sharma-Tasso-Olver-Burgers (STOB) system is analyzed by the Lie point symmetry method. The hypergeometric wave solution of the STOB equation is derived by symmetry reductions. In the meantime, the consistent tanh expansion (CTE) method is applied to the STOB equation. An nonauto-Bäcklund (BT) theorem that includes the over-determined equations and the consistent condition is obtained by the CTE method. By using the nonauto-BT theorem, the interactions between one-soliton and the cnoidal wave, and between one-soliton and the multiple resonant soliton solutions, are constructed. The dynamics of these novel interaction solutions are shown both in analytical and graphical forms. The results are potentially useful for explaining ocean phenomena.
Numerical Study on Thermal and Flow Characteristics of Divergent Duct with Different Rib Shapes for Electric-Vehicle Cooling System
Authors: Garud Kunal Sandip , Kudriavskyi Yurii , Lee Myung-Sung , Kang Eun-Hyeok , Lee Moo-Yeon
Published: August 2022

The cooling performance of the air-conditioning system in electric vehicles could be enhanced through the geometrical optimization of the air ducts. Furthermore, it has been proven that the heat-transfer performance of divergent channels is better than that of conventional channels. Therefore, the present study investigates the thermal and flow characteristics of divergent ducts with various rib shapes for the cooling system of electric vehicles. The thermal and flow characteristics, namely, temperature difference, pressure drop, heat-transfer coefficient, Nusselt number and friction factor, are numerically studied. Divergent ducts comprising ribs with the different shapes of rectangle, isosceles triangle, left triangle, right triangle, trapezoid, left trapezoid and right trapezoid arranged symmetrically are modeled as the computational domains. The thermal and flow characteristics of divergent ducts with various rib shapes are simulated in ANSYS Fluent commercial software for the Reynolds-number range of 22,000–79,000. The numerical model is validated by comparing the simulated results with the corresponding experimental results of the Nusselt number and the friction factor, obtaining errors of 4.4% and 2.9%, respectively. The results reveal that the divergent duct with the right-triangular rib shape shows the maximum values of the heat-transfer coefficient and Nusselt number of 180.65 W/m2K and 601, respectively. The same rib shape shows a pressure drop and a friction factor of 137.3 Pa and 0.040, respectively, which are lower than those of all rib shapes, except for the trapezoidal and right-trapezoidal rib shapes. Considering the trade-off comparison between thermal and flow characteristics, the divergent duct with the right-triangular rib shape is proposed as the best configuration. In addition, the effect of various conditions of the inlet air temperature on the thermal characteristics of the best configuration is discussed. The proposed results could be considered to develop an air-duct system with enhanced efficiency for electric vehicles.
Early Isolates of SARS-CoV-2 Result in Different Pathogenesis in the Transduced Mouse Model of COVID-19
Authors: Smither Sophie J. , Kempster Sarah , Priestnall Simon L. , Suárez-Bonnet Alejandro , Stapleton Helen , Laws Thomas R. , Ferguson Deborah , Almond Neil , Lever Mark S. , Williamson E. Diane
Published: August 2022

A transduced mouse model of SARS-CoV-2 infection was established using Balb/c mice. This was achieved through the adenovirus-vectored delivery of the hACE2 gene, to render the mice transiently susceptible to the virus. The model was characterised in terms of the dissemination of hACE2 receptor expression, the dissemination of three SARS-CoV-2 virus variants in vivo up to 10 days following challenge, the resulting histopathology and the clinical signs induced in the mice. In transduced mice, the infection was short-term, with a rapid loss in body weight starting at day 2 with maximum weight loss at day 4, followed by subsequent recovery until day 10. The induced expression of the hACE2 receptor was evident in the lungs, but, upon challenge, the SARS-CoV-2 virus disseminated beyond the lungs to spleen, liver and kidney, peaking at day 2 post infection. However, by day 10 post infection, the virus was undetectable. The lung histopathology was characterised by bronchial and alveolar inflammation, which was still present at day 10 post infection. Transduced mice had differential responses to viral variants ranking CVR-Glasgow 1 > Victoria-1 > England-2 isolates in terms of body weight loss. The transduced mouse model provides a consistent and manipulatable model of SARS-CoV-2 infection to screen viral variants for their relative virulence and possible interventions.
Rotavirus Strain Trends in United States, 2009–2016: Results from the National Rotavirus Strain Surveillance System (NRSSS)
Authors: Mijatovic-Rustempasic Slavica , Jaimes Jose , Perkins Charity , Ward M. Leanne , Esona Mathew D. , Gautam Rashi , Lewis Jamie , Sturgeon Michele , Panjwani Junaid , Bloom Gail A. , Miller Steve , Reisdorf Erik , Riley Ann Marie , Pence Morgan A. , Dunn James , Selvarangan Rangaraj , Jerris Robert C. , DeGroat Dona , Parashar Umesh D. , Cortese Margaret M. , Bowen Michael D.
Published: August 2022

Before the introduction of vaccines, group A rotaviruses (RVA) were the leading cause of acute gastroenteritis in children worldwide. The National Rotavirus Strain Surveillance System (NRSSS) was established in 1996 by the Centers for Disease Control and Prevention (CDC) to perform passive RVA surveillance in the USA. We report the distribution of RVA genotypes collected through NRSSS during the 2009–2016 RVA seasons and retrospectively examine the genotypes detected through the NRSSS since 1996. During the 2009–2016 RVA seasons, 2134 RVA-positive fecal specimens were sent to the CDC for analysis of the VP7 and VP4 genes by RT-PCR genotyping assays and sequencing. During 2009–2011, RVA genotype G3P[8] dominated, while G12P[8] was the dominant genotype during 2012–2016. Vaccine strains were detected in 1.7% of specimens and uncommon/unusual strains, including equine-like G3P[8] strains, were found in 1.9%. Phylogenetic analyses showed limited VP7 and VP4 sequence variation within the common genotypes with 1–3 alleles/lineages identified per genotype. A review of 20 years of NRSSS surveillance showed two changes in genotype dominance, from G1P[8] to G3P[8] and then G3P[8] to G12P[8]. A better understanding of the long-term effects of vaccine use on epidemiological and evolutionary dynamics of circulating RVA strains requires continued surveillance.
Construction and Evaluation of Recombinant Adenovirus Candidate Vaccines for Chikungunya Virus
Authors: Cao Liang , Wang Wei , Sun Wenchao , Zhang Jinyong , Han Jicheng , Xie Changzhan , Ha Zhuo , Xie Yubiao , Zhang He , Jin Ningyi , Lu Huijun
Published: August 2022

Chikungunya virus (CHIKV) is a mosquito-borne virus. The emergence of CHIKV infection has raised global concern, and there is a growing need to develop safe and effective vaccines. Here, adenovirus 5 was used as the vaccine vector to construct recombinant adenoviruses expressing CHIKV E2, E1, and E2-6K-E1, respectively. And then the immunogenicity and protective efficiency against CHIKV were evaluated in BALB/c mice. Compared to the ad-wt control group, all three vaccines elicited significant humoral and cellar immune responses. The levels of neutralizing antibodies in the rAd-CHIKV-E2-6K-E1 and rAd-CHIKV-E2 groups both reached 1:256, which were 3.2 times higher than those in the rAd-CHIKV-E1 group. Furthermore, the levels of lymphocyte proliferation in rAd-CHIKV-E2-6K-E1 group were the highest. Besides, the concentrations of IFN-γ and IL-4 in mice immunized with rAd-CHIKV-E2-6K-E1 were 1.37 and 1.20 times higher than those in ad-wt immunized mice, respectively. After the challenge, mice in the rAd-CHIKV-E2-6K-E1 and rAd-CHIKV-E2 groups lost 2% of their body weight compared with 5% in the ad-wt control group. And low viral loads were detected in the heart, kidney, and blood of mice immunized with rAd-CHIKV-E2-6K-E1 and rAd-CHIKV-E2 at 3–5 dpc, which decreased by 0.4–0.7 orders of magnitude compared with the ad-wt control. Overall, these data suggest that the recombinant adenovirus is a potential candidate vaccine against CHIKV.
Porcine Enteric Coronavirus PEDV Induces the ROS-ATM and Caspase7-CAD-γH2AX Signaling Pathways to Foster Its Replication
Authors: Ming Xin , Chen Huan , Yang Ying , Zhao Pu , Sun Liumei , Zhang Caisheng , Shin Hyun-Jin , Lee Jeong-Soo , Jung Yong-Sam , Qian Yingjuan
Published: August 2022

DNA damage response (DDR) is an evolutionarily conserved mechanism by which eukaryotic cells sense DNA lesions caused by intrinsic and extrinsic stimuli, including virus infection. Although interactions between DNA viruses and DDR have been extensively studied, how RNA viruses, especially coronaviruses, regulate DDR remains unknown. A previous study showed that the porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus in the Coronaviridae family, induces DDR in infected cells. However, the underlying mechanism was unclear. This study showed that PEDV activates the ATM-Chk2 signaling, while inhibition of ATM or Chk2 dampens the early stage of PEDV infection. Additionally, we found that PEDV-activated ATM signaling correlates with intracellular ROS production. Interestingly, we showed that, unlike the typical γH2AX foci, PEDV infection leads to a unique γH2AX staining pattern, including phase I (nuclear ring staining), II (pan-nuclear staining), and III (co-staining with apoptotic bodies), which highly resembles the apoptosis process. Furthermore, we demonstrated that PEDV-induced H2AX phosphorylation depends on the activation of caspase-7 and caspase-activated DNAse (CAD), but not ATM-Chk2. Finally, we showed that the knockdown of H2AX attenuates PEDV replication. Taken together, we conclude that PEDV induces DDR through the ROS-ATM and caspase7-CAD-γH2AX signaling pathways to foster its early replication.
Baculovirus Display of Varicella–Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in Mice
Authors: Xue Wenhui , Li Tingting , Zhang Sibo , Wang Yingbin , Hong Minqing , Cui Lingyan , Wang Hong , Zhang Yuyun , Chen Tingting , Zhu Rui , Chen Zhenqin , Zhou Lizhi , Zhang Rongwei , Cheng Tong , Zheng Qingbing , Zhang Jun , Gu Ying , Xia Ningshao , Li Shaowei
Published: August 2022

Varicella–zoster virus (VZV) is the causative agent of varicella and herpes zoster (HZ) and can pose a significant challenge to human health globally. The initial VZV infection—more common in children—causes a self-limiting chicken pox. However, in later life, the latent VZV can become reactivated in these patients, causing HZ and postherpetic neuralgia (PHN), a serious and painful complication. VZV glycoprotein E (gE) has been developed into a licensed subunit vaccine against HZ (Shingrix). However, its efficacy relies on the concomitant delivery of a robust adjuvant (AS01B). Here, we sought to create a new immunogen for vaccine design by displaying the VZV–gE on the baculovirus surface (Bac–gE). Correct localization and display of gE on the engineered baculovirus was verified by flow cytometry and immune electron microscopy. We show that Bac–gE provides excellent antigenicity against VZV and induces not only stronger gE-specific CD4+ and CD8+ T cell responses but also higher levels of VZV–specific neutralizing antibodies as compared with other vaccine strategies in mice. Collectively, we show that the baculovirus display of VZV–gE confers ideal humoral and cellular immune responses required for HZ vaccine development, paving the way for a baculovirus-based vaccine design.
Transjugular Patent Ductus Arteriosus Occlusion in Seven Dogs Using the Amplatzer Vascular Plug II
Authors: Bagardi Mara , Domenech Oriol , Vezzosi Tommaso , Marchesotti Federica , Bini Martina , Patata Valentina , Croce Marta , Valenti Valentina , Venco Luigi
Published: August 2022

Although vascular plugs for the closure of patent ductus arteriosus (PDA) have been validated in dogs, studies are lacking on its use as a first-choice device with a transjugular approach. The present case series describes the transvenous right jugular embolization of PDA using an Amplatzer Vascular Plug II in seven dogs of different ages, breeds, and body weights. Complete closure of the PDA was demonstrated in all cases. All dogs showed significant hemodynamic reduction of pulmonary overcirculation and left heart size after the procedure and at following echocardiographic check-ups. Transjugular PDA occlusion using an Amplatzer Vascular Plug II can thus be considered as a safe alternative to the arterial or venous femoral approach using an Amplatzer canine ductal occluder (ACDO), particularly in puppies with small femoral vessels.
Development of a Protocol for Biomechanical Gait Analysis in Asian Elephants Using the Triaxial Inertial Measurement Unit (IMU)
Authors: Wantanajittikul Kittichai , Thitaram Chatchote , Khammesri Siripat , Kongsawasdi Siriphan
Published: August 2022

Gait analysis is a method of gathering quantitative information to assist in determining the cause of abnormal gait for the purpose of making treatment decisions in veterinary medicine. Recent technology has offered the wearable wireless sensor of an inertial measurement unit (IMU) for determining gait parameters. This study proposed the use of a triaxial IMU, comprising an accelerometer, a gyroscope, and a magnetometer, for detecting three-dimensional limb segment motion (XYZ axis) during the gait cycle in Asian elephants. A new algorithm was developed to estimate the kinematic parameter that represents each limb segment of the forelimbs and hindlimbs while walking at a comfortable speed. For future use, this study aimed to create a new prototype of the IMU with a configuration that is tailored to the elephant and apply machine learning in an effort to achieve greater precision.
Peak Shaving for Electric Vehicle Charging Infrastructure—A Case Study in a Parking Garage in Uppsala, Sweden
Authors: Wallberg Alexander , Flygare Carl , Waters Rafael , Castellucci Valeria
Published: August 2022

The need for a more flexible usage of power is increasing due to the electrification of new sectors in society combined with larger amounts of integrated intermittent electricity production in the power system. Among other cities, Uppsala in Sweden is undergoing an accelerated transition of its vehicle fleet from fossil combustion engines to electrical vehicles. To meet the requirements of the transforming mobility infrastructure, Uppsala municipality has, in collaboration with Uppsala University, built a full-scale commercial electrical vehicle parking garage equipped with a battery storage and photovoltaic system. This paper presents the current hardware topology of the parking garage, a neural network for day-ahead predictions of the parking garage’s load profile, and a simulation model in MATLAB using rule-based peak shaving control. The created neural network was trained on data from 2021 and its performance was evaluated using data from 2022. The performance of the rule-based peak shaving control was evaluated using the predicted load demand and photovoltaic data collected for the parking garage. The aim of this paper is to test a prediction model and peak shaving strategy that could be implemented in practice on-site at the parking garage. The created neural network has a linear regression index of 0.61, which proved to yield a satisfying result when used in the rule-based peak shaving control with the parking garage’s 60 kW/137 kWh battery system. The peak shaving model was able to reduce the highest load demand peak of 117 kW by 38.6% using the forecast of a neural network.
Design of an Intelligent Platoon Transit System towards Transportation Electrification
Authors: Gao Hong , Li Ang , Wang Jiangbo , Liu Kai , Zhang Li
Published: August 2022

The full implementation of electric public transport is a key step for the transport industry to move toward electrification and achieve carbon neutrality. However, in the face of time-varying demands and high-quality service requirements, traditional transit systems are difficult to ascend as the preferred mode of travel due to the constraints of fixed vehicle capacity and multiline transfers. With the advent of modular vehicle technology, it is becoming more realistic to develop an entirely new transportation system based on electric modular vehicles (EMVs). This study proposes a novel intelligent platoon transit system (IPTS), and its overall concept and operating mode are elaborated at a strategic level. In particular, the electrical, modular, and autonomous platoon transit system should be designed to achieve adaptive adjustment of capacity and possible en route transfers, which significantly improves the convenience, flexibility, and economy of public transport. We also design three application scenarios with varying demands during multistage development to bridge the gap in traditional buses. The key issues and case applicability of the three scenarios are discussed.
Analysis of Electric Vehicle Battery State Estimation Using Scopus and Web of Science Databases from 2000 to 2021: A Bibliometric Study
Authors: Swarnkar Radhika , Harikrishnan R. , Singh Mangal
Published: August 2022

This paper presents a bibliometric analysis of battery state estimation in electric vehicles. In this paper, a quick study is performed on the top global research contributors, funding agencies, and affiliate universities or institutes performing research on this topic while also finding the top keyword searches and top authors based on the most citations in the field of electric vehicles. Trend analysis is done by using the SCOPUS and Web of Science (WOS) databases (DB) from the period of 2000 to 2021. Battery state estimation plays a major role in the battery present state based on past experience. Battery available charge and health knowledge is a must for range estimation and helps us acknowledge if a battery is in useful condition or needs maintenance or replacement. A total of 136 documents in SCOPUS and 1311 documents in Web of Science were analyzed. Through this bibliometric analysis, we learn the top authors, country, publication journal, citation, funding agency, leading documents, research gap, and future trends in this research direction. The author Xiong Rui has the most publications, and he is working at the Beijing Institute of Technology, China. The most common institution is the Beijing Institute of Technology, and China is the most highly contributing country in this research. Most of the publications are conference types in SCOPUS DB and article types in WOS DB. The National Natural Science Foundation of China provides the most funding. The journal Energies has the most publications related to this field. The most cited works are by the authors M.A. Hannan and L.G. Lu in SCOPUS and WOS DB, respectively. A statistical analysis of the top ten countries’ productivity results is also discussed.
The political situation after the political upheavals in Kyrgyzstan
Authors:   National Academy of Sciences of the Kyrgyz Republic, Zhorobekov Z. Z. , Aytiev S. A. ,   Bishkek Osh State University, Kyrgyz Republic, Bishkek, Imanbayev S. K. ,   Kyrgyz National University named after Zh. Balasagyn, KyrKyrgyz Republic, Bishkek
Published: August 2022

This article is written on the political situation after the coup of the political regime of the Kyrgyz Republic in 2005–2010. Of particular interest are works devoted to the analysis of crisis political situations. Recently, in connection with the rapid development of political sciences in the country, there has been a need to study the political situation as an integral system. In a number of works, it was considered as a part, a link in the political process, and the conceptual apparatus was specified. However, until now, the political situation itself, its essence, structure, and mechanism of functioning have not been studied comprehensively and holistically.
Psychological trust in social networks
Authors:   Bishkek International University Ala-Too, Kyrgyz Republic, Zhakshylykova T. A.
Published: August 2022

The article analyzes the influence of the social network on humanity. For many years, humanity has realized even in social networks the need for psychological security. Social media confrontation is psychological warfare on a virtual level. Expecting negative information on a social network, people communicate only with friends, like-minded people. Communication on the social network has intensified over the past three years, due to the pandemic in the world, minimal contact has entered the way of life of mankind. Nevertheless, any positive and negative information can affect the psychological state of a person. Therefore, security at the psychological level is necessary and requires new approaches of non-violence.
Social networks in the work of the government: advantages, problems and ways of using them
Authors:   K. Dikambayev Diplomatic Academy of the Ministry of Foreign Affairs of the Kyrgyz Republic, Abdyrashev A. , Alimakhunov A. ,   K. Dikambayev Diplomatic Academy of the Ministry of Foreign Affairs of the Kyrgyz Republic
Published: August 2022

In this article, the author discusses the use of social networks as a tool for informing the public about the process of government work. The authors characterize social networks as an actual way of lobbying political interests in society, the distinctive characteristics of which are: a wide variety of approaches, focus on the needs of the target audience, efficiency, flexibility and low costs. The article discusses the approaches to the work of social networks presented at the present stage of the development of the Internet, as well as the rapid dissemination and receipt of information through new media. The author suggests the correct practice of using social networks in the work of executive authorities, as well as the professional use of technologies and tools for electronic interaction necessary in teaching digital competencies and mastering best practices. The results can be useful in developing a policy of state bodies in social networks, recommendations for the interaction of civil servants with society in the digital space.
A New Paradigm for the Study of Christian Origins: Replacing the Dendritic Model
Authors: Zindler Frank R.
Published: August 2022

This article argues that the dendritic (tree-like) or traditional model of Christian origins must be replaced with a plectic (braid-like) model. The dendritic model assumes that Christianity began at a specific point in both time and space—in the person of “Jesus of Nazareth”—and then branched out to form the various ancient sects of Christianity. This article asks: What if the numerous forms of “Christianity” did not all derive from a single historical figure? What if these earliest “Christianities” arose in the same way that the different forms and varieties of Egyptian, Indic, and Greco-Roman religions evolved? A new paradigm is proposed where the various forms of Christianity can be envisioned as forming by the coalescence of various threads (or trajectories) of religious tradition. Some of the threads may trace back into the mists of prehistory, others may trace to the turn of the current era, and still others may have begun in the second or third centuries CE. Not all early forms of Christianity contained the same threads. Not all threads stayed in the braid for long, and still others continued into the present. After entering the braid, threads of tradition evolved, bifurcated, branched off, or were absorbed into other traditions. Clearly, this is what we see happening today as multitudinous sects, cults, and denominations continue to arise and go extinct. As in historical geology, so too in religious history: The present is the key to the past.
Sistema de gestión de la investigación de la Escuela Politécnica Nacional (EPN) Ecuador.
Authors: Paredes Vasco Norma , Chaparro Martínez Exio , Morales Intriago Juan
Published: August 2022

El presente trabajo se centra en los CRIS (Current Research Information System) o Sistemas de Gestión de la Investigación, especialmente en los de tipo institucional. En primer lugar, se realiza un análisis documental sobre los sistemas de gestión de la investigación en varias universidades. La necesidad de este trabajo se fundamenta en profundizar el estudio de los sistemas de gestión de la investigación de la Escuela Politécnica Nacional (EPN) a partir de la búsqueda bibliográfica aplicada a los sistemas de biblioteca de la institución como punto de partida. El objetivo principal de este estudio es proponer un sistema para la gestión de la investigación tomando como base trabajos, artículos, que contengan procesos claves y aspectos necesarios para la implementación y/o activación del CRIS de la EPN. La metodología aplicada consiste en una investigación cualitativa y no experimental, para definir criterios, bases y/o políticas que constituyan el enfoque principal de un Sistema de Gestión de la Investigación. Los resultados de la búsqueda y posterior propuesta determinan un eje de enfoque generalizado que incluye al personal académico, administrativo y de apoyo que desarrollen roles dentro del sistema. Como conclusión se marca el camino para proponer un sistema de gestión de la investigación para la EPN con los aspectos, criterios y/o políticas necesarias para organizar la actividad científica institucional, considerando los ejes: principales investigadores, organizaciones, proyectos y resultados de la investigación   .
Relato de experiência estágio extra curricular – acadêmicos de enfermagem na UPA - Unidade de Pronto Atendimento em 2021
Authors: Mota Renata dos Santos , Bidu Alec de Assunção , Azevedo João Victor Cardoso , Almeida Paloma Luiza Santos de , Abreu Marcos Ramos
Published: August 2022

Introdução  O Sistema  de  Saúde,  no  Brasil,  encontra-se  estruturado  em  três níveis hierárquicos complementares de atenção à saúde − atenção básica,de  média  e  alta  complexidade.  As  Unidades  de  Pronto  Atendimento (UPA) fazem  parte  da  Política Nacional  de  Urgência  e  Emergência,  lançada  pelo Ministério da Saúde em 2003, com o intuito de integrar a atenção às urgências. A UPA e a prefeitura da cidade de Itabuna viabilizaram um estágio extracurricular para os acadêmicos de enfermagem, em vista da necessidade de ampliar a visibilidade e promover conhecimento e vivências de assistência de enfermagem ainda na academia. Objetivo: Pretende-se relatar a experiência de acadêmicos de enfermagem nas atividades desenvolvidas no estágio extracurricular na Unidade de pronto atendimento.  Métodos: O estágio ocorreu no período de novembro 2021 a março de 2022, com plantões de 12/24 horas semanais. Tendo como base o protocolo de acolhimento  com  avaliação  e  classificação  de  risco  a  predominância  dos atendimentos não urgentes e de baixas complexidades. No período inicial do estágio, vivenciamos ainda a pandemia da COVID 19, onde a UPA era o local no município onde se faziam as consultas de pessoas que apresentavam sintomas gripais. Resultados: Durante as vivências, foram presenciados atendimentos a pacientes com Covid e h3n2, crises convulsivas e metabólicas, acidente vascular encefálico, paradas cardiorrespiratórias e outros. Como resultado, os acadêmicos perceberam a importância a qual os profissionais enfermeiros precisam estar capacitados para o atendimento de urgência, tema pouco abordado durante a formação acadêmica. Conclusão: Dessa forma, a vivência permitiu aos estudantes refletirem sobre a prática do acolhimento com classificação de risco e entender o papel do enfermeiro como agente transformador nesse processo. Ressalta-se ainda que o estágio no serviço, sob a forma de plantões foi bastante favorável e serviu para o melhor entendimento do conteúdo teórico estudado e para o treinamento prático, e evidenciou que a parceria de instituições como a UPA e instituições de ensino são necessárias para a formação do acadêmico em saúde. O estágio foi muito significativo no aprendizado dos educandos de enfermagem sobre a rede urgências e emergências.
Molecular identification of non-conventional yeasts and the screening for α-terpineol assimilation
Authors: Dos Santos Mariely Cristina , Do Nascimento Mariana Machado Fidelis , Hashimoto Elisabete Hiromi , Bittencourt Juliana Vitória Messias
Published: August 2022

The present research aimed to analyze the potential consumption of a monoterpene compound, α-terpineol, by aroma-producing Yeasts. For this purpose, 23 non-conventional yeasts were selected and identified by molecular tools. Subsequently, a screening test was performed to evaluate their tolerance to α-terpineol, by cultivating them in YM broth, at pH 5.0, for 48 h. A mixture of α-terpineol and ethyl alcohol was added to the broth in concentrations ranging from 2.5 to 10.0 μL/mL (intervals of 2.5). The yeasts that survived any of these concentrations were cultured again, but this time in mineral DP liquid culture medium to assess the consumption of α-terpineol. TLC analysis was conducted to analyze the consumption of α-terpineol. The yeasts studied were identified as Clavispora lusitaniae (n=12), Rhodotorula mucilaginosa (n=8), and Lodderomyces elongisporus (n=3). Furthermore, according to the phylogenetic tree, two of these strains (C. lusitaniae and L. elongisporus) have greater genetic proximity than the yeast R. mucilaginosa. From the 23 yeasts, 6 of them were shown to be tolerant to the concentration of 2.5 μL/mL of α-terpineol. The tolerant strains were identified as C. lusitaniae (n = 1), L. elongisporus (n = 2), and R. mucilaginosa (n = 3). However, the yeast that resisted the highest concentration, R. mucilaginosa, was only 12 hours in all concentrations. It is more likely that the defense mechanism of the yeast was not able to prevent further damage to the membrane as the time in contact with α-terpineol increased. The TLC results showed that the extracts from R. mucilaginosa (CMRP3205) and L. elongisporus (CMRP3192) could be interpreted as potentially promising results of new compounds production by the yeasts.
Magnetometric Investigations of Biomagnetic Signals: Magnetocardiography
Authors: Primin Mykhailo , Nedayvoda Igor
Published: August 2022

Introduction. Superconducting magnetometers based on SQUIDs (SQUID- Superconducting QUantum Interference Device) are currently used to register weak magnetic fields generated in various human organs and measured outside the body (in the environment). The creation of information technology, which is a set of methods and software tools combined into a technological chain that ensures registration, storage, pre-processing, analysis of measurement data and automatic diagnostic output, is an essential science-intensive component that determines the possibilities and success of the applied use of non-contact diagnostic systems of the human heart The purpose. Article presents new algorithms for spatial analysis of cardiomagnetic signal measurement results. The algorithms are based on the inverse problem solution, when the magnetic field source is matched to the spatial distribution of the magnetic signal and the parameters and spatial configuration of the source are determined. A model of the cardiomagnetic source was used in the form of a system of current density vectors, which are distributed in a plane that is parallel to the measurement plane and crosses the volume of the heart. Results. The inverse problem is solved using the apparatus of two-dimensional integral Fourier transformations. The data transformation algorithm allows to correctly take into account the design of the magnetic flux transformer (the dimensions of the pickup coils, their spatial location and the electrical connection scheme). Algorithm modifications have been developed for most of the known (implemented in existing magnetocardiographs) designs of magnetic flux transformers of the first and second order gradientometers. The operation of the algorithm is modeled on real data of magnetometric investigations of the human heart. Investigations have shown that the application of the proposed algorithms allows obtaining new information about the spatial configuration of the magnetic signal source in the human heart, which can be used in the future for the diagnosis of human heart diseases. Keywords: magnetocardiography, inverse problem of magnetostatics, Fourier transform, SQUID gradientometer.
Evaluation the Predictive Significance Signs for Metastatic Involvement of Lymph Nodes in Cervical Cancer According to Magnetic Resonance Imaging Findings
Authors: Жук Е.Г. , Косенко И.А.
Published: August 2022

Цель. Разработать новые признаки метастатического поражения лимфатических узлов (ЛУ) по данным магнитно-резонансного исследования (МРТ) при раке шейки матки (РШМ) и оценить их прогностическую значимость.Материалы и методы. Данные МРТ 71 пациента с гистологически подтвержденным диагнозом РШМ, которому выполнено радикальное хирургическое лечение со стандартной лимфаденэктомией (ЛАЭ), сопоставляли с результатами морфологического исследования резецированных препаратов. С целью оценки прогностической значимости МРТ-признаков метастатического поражения ЛУ был проведен моновариантный регрессионный логистический анализ; для оценки диагностической эффективности методов и определения порога отсечения проведен ROC-анализ.Результаты. Установлено, что клинически и статистически значимыми предикторами метастатического поражения ЛУ являются: размер ЛУ по короткой оси, соотношение размера ЛУ по длинной и короткой оси (индекс конфигурации), отсутствие сигнала от жировой ткани ворот ЛУ, наличие шиповатости контура ЛУ, наличие перинодулярного отека, субкапсулярного и центрального отека ЛУ, неоднородность МР-сигнала ЛУ, значение коэффициента интенсивности сигнала ЛУ и значение измеряемого коэффициента диффузии (ИКД) ЛУ (р<0,05).Заключение. Установлена прогностическая значимость МРТ-предикторов метастатического поражения ЛУ при РШМ.

Purpose. To develop new MRI signs for metastatic involvement of the lymph nodes (LN) in cervical cancer (СС) аnd to evaluate of their predictive significance.Materials and methods. The MRI findings of the 71 patients with histologically confirmed diagnosis of cervical cancer, who underwent radical surgical treatment with lymphatic dissection, were compared with the morphological features. To evaluate the predictive significance of MRI signs of metastatic LN involvement, uni-variate regression logistic analyses was performed. To evaluate the diagnostic performance of methods and to determine the cutoff values, ROC-analysis was perfomed.Results. It was found, that clinically and statistically significant predictors of metastatic involvement of the LN are: the size of the LN along the short axis, the configuration index (ratio between the long and short axis of LN), the absence of a signal from the adipose tissue of the LN hilus, prickly outline of the LN, the presence of perinodular edema of LN, subcapsular and central edema of LN, the presence of heterogeneity of LN MR signal, the signal intensity coefficient of LN tissue and tumor (ISLN/IS tumor) and apparent diffusion coefficient (ADC) of LN (p<0.05).Conclusions. The prognostic significance of predictors of LN metastatic involvement in cervical cancer was established.
Influence of gender on the risk of death of radically operated patients with non-small cell lung cancer
Authors: Курчин В.П. , Гапеенко В.В. , Кузнецова Т.А.
Published: August 2022

Цель. Оценка влияния пола на выживаемость радикально оперированных пациентов с НМРЛ I–IIIA стадии и целесообразность включения этой переменной в прогностическую модель.Материалы и методы. Материалом для исследования послужила база данных с клиническими данными 697 радикально оперированных пациентов с немелкоклеточным раком легкого (НМРЛ) I–IIIA стадии за период с 2000 по 2010 г. Для моделирования зависимости относительного риска смерти от пола и других факторов после радикального лечения использовался регрессионный анализ пропорциональных рисков Кокса. Оценка качества прогностических моделей, включающих переменную«пол», проводилась с помощью статистики «минус 2 логарифм отношения правдоподобия» (–2LL).Результаты. Общая 5-летняя выживаемость во всей выборке равнялась 39,1±1,9%. 5-летняя выживаемость была выше у женщин и составила 53,5±6,6% против 37,8±1,9% у мужчин. Для оценки этого предиктора была создана одномерная модель с переменной «пол», установлена статистическая достоверность влияния пола на выживаемость, однако при усложнении модели путем последовательного добавления в нее переменных значимость переменной «пол» нивелировалась, по результатам теста«–2LL» усложнение модели данной переменной не улучшает ее подгонку к данным. Заключение. Пол в совокупности с другими предикторами в рамках модели выживаемости пациентов не улучшает ее подгонку к данным.

Purpose. To assess the effect of gender on the survival rate of radically operated patients with stage I–IIIA NSCLC and the feasibility of including this variable in the prognostic model.Methods. The material for the study was a database with clinical data of 697 radically operated patients with stage I–IIIA non-small cell lung cancer (NSCLC) for the period from 2000 to 2010. We used Cox proportional hazards regression analysis to model the dependence of the relative risk of death on gender and other factors after radical treatment. Assessment of the quality of predictive models that include the variable "sex" was carried out using the statistic "minus 2 log likelihood ratio" (–2LL).Results. The overall 5-year survival rate in the entire sample was 39.1±1.9%. 5-year survival rate is higher in women and amounted to 53.5±6.6% versus 37.8±1.9% in men. To assess this predictor, a one-dimensional model was created with the variable "sex", the statistical significance of the influence of sex on survival was established, however, when the model was made more complex by successively adding variables to it, the significance of the variable "sex" was leveled, according to the results of the test "–2LL" the complication of the model of this variable does not improve its fit to the data.Conclusion. Gender in the aggregate of other predictors in the patient survival model does not improve its fit to the data.
PARÁMETROS GENÉTICOS Y EFICIENCIA DE LA SELECCIÓN TEMPRANA EN Pinus ayacahuite EHREN. VAR. ayacahuite
Authors: Farfán-Vázquez Emanuel de Guadalupe , Jaso-Mata Jesús , López-Upton Javier , Vargas-Hernández J. Jesús , Ramírez-Herrera Carlos
Published: August 2022

Con el propósito de evaluar la eficiencia de la selección temprana en Pinus ayacahuite Ehren. var. ayacahuite, se estimó el control genético en el crecimiento en altura y diámetro de los árboles en edades de 3 a 13 años, así como las correlaciones genéticas edad-edad en un ensayo con 42 familias de polinización libre. En el ensayo se incluyeron dos grupos de poblaciones, un grupo del Sur de México y otro de Centroamérica. En ambas características se presentó una amplia variación genética, tanto entre grupos como entre familias dentro de grupos. La heredabilidad individual para la altura total y el diámetro a la base del fuste varió de 0.31 a 0.54 con la edad, y a nivel de familia, de 0.71 a 0.83. Además se encontró una fuerte correlación genética entre la altura y el diámetro a la base (rGxy = 0.90) a los 13 años de edad, por lo que al utilizar la altura o el diámetro como criterio de selección a esa edad, se obtiene prácticamente la misma respuesta. Las correlaciones genéticas edad-edad para las características evaluadas a diferentes edades (3, 4, 5 y 13 años) fueron altas en general y ascendentes con el tiempo (de 0.72 a 0.78 para altura y de 0.75 a 0.85 para el diámetro). Debido a lo anterior, la selección temprana (a los 3 años de edad) tendría una eficiencia cercana a 60 % con respecto a la respuesta de la selección directa a los 13 años de edad.
LA SELECCIÓN MASAL COMO MÉTODO PARA OBTENER LÍNEAS DE ALTA APTITUD COMBINATORIA ESPECÍFICA EN MAÍZ
Authors: García-Zavala Jesús , Molina-Galán José , López-Reynoso Jesús
Published: August 2022

Con los objetivos de probar que la variedad de maíz (Zea mays L.) México Grupo 10, mejorada mediante seis ciclos de selección masal estratificada (C6), tiene mayor capacidad que su versión original (C0) para producir líneas autofecundadas de alto rendimiento y de alta aptitud combinatoria general (ACG) y específica (ACE), y de estimar la correlación fenotípica entre el rendimiento per se de las líneas y su ACG, se derivaron 30 líneas de tres autofecundaciones (S3) de C0 y 30 líneas S3 de C6, con las cuales se formaron dos conjuntos de 30 mestizos cada uno, utilizando como probador a C0. También se obtuvieron 25 cruzas simples en cadena (1x2, 2x3,...,25x1) dentro de cada conjunto de líneas. Cada grupo, de 60 líneas S3, de 60 mestizos y de 50 híbridos simples, se evaluó durante el ciclo agrícola de 1998, en dos localidades del Estado de México, en condiciones de riego, en un diseño experimental de bloques completos al azar con cuatro repeticiones. Los resultados obtenidos indicaron que la variedad mejorada C6, produjo mayor frecuencia de líneas autofecundadas de alta ACE, y por tanto más híbridos de alto rendimiento, que C0. En cambio, la mayor frecuencia de líneas de mayor rendimiento per se, aunque no siempre las de más alta ACG, provinieron de C0. Por ello resultó un valor de correlación muy bajo (r = 0.10) entre el rendimiento y la ACG de las líneas.
CONTROL DE MOSQUITA BLANCA Y VIROSIS EN JITOMATE CON CUBIERTA FLOTANTE EN VERACRUZ
Authors: Domínguez-Torres Armando , García-Pérez Eliseo , Pacheco-Velasco José Evaristo , Villanueva-Jiménez Juan A. , Téliz-Ortíz Daniel
Published: August 2022

La mosquita blanca (Bemisia tabaci) en jitomate (Lycopersicon esculentum) en México ocasiona daño directo y transmite enfermedades virales, que reducen el potencial productivo. Para su protección se pueden utilizar cubiertas flotantes (malla de polipropileno). Sin embargo, por el microclima que generan deben probarse en cada condición agroecológica. El objetivo fue determinar los periodos de cobertura que optimicen las ventajas de la cubierta en la zona centro de Veracruz, México. El trabajo de campo se realizó de diciembre de 1994 a abril de 1995 en el Municipio de Manlio F. Altamirano, Veracruz. Los factores evaluados fueron periodos de cobertura de 0 (testigo), 35, 40 y 45 días después del trasplante y dos métodos de control de malezas, químico y manual. Se estableció un diseño en bloques al azar con tres repeticiones en arreglo factorial. Se registró la fluctuación poblacional de mosquita blanca, incidencia de virosis, altura de planta, rendimiento y rentabilidad. Todos los periodos de cobertura probados favorecieron el crecimiento de la planta, disminuyeron la incidencia de virosis e incrementaron el rendimiento con relación al testigo, en ambos sistemas de control de maleza, con promedios de 25.0, 79.6 y 49.0 %, respectivamente. Los tratamientos que incluyeron periodos de cobertura de 35 y 40 días con control manual de maleza presentaron los mayores beneficios económicos ($12 689 y $12 902, respectivamente) y las mejores tasas de retorno (54.7 y 55.6 %, respectivamente).
RENDIMIENTO Y COMPOSICIÓN QUÍMICA DEL FORRAJE DE HUIZACHILLO (Desmanthus virgatus L. var. depressus Willd) BAJO CONDICIONES DE CULTIVO
Authors: Zamora-Natera Francisco , Martínez-Rodríguez Maurilio , Ruiz-López Mario , García-López Pedro
Published: August 2022

La explotación de bovinos y caprinos es una actividad económica importante en las zonas áridas del estado de Nuevo León, México. Sin embargo, está limitada por la baja producción de forraje debido a las condiciones ambientales adversas en estas regiones. Una alternativa potencial es incorporar al cultivo especies nativas con valor forrajero. Se realizó un experimento en Marín, Nuevo León, durante 1995, con el objetivo de evaluar la producción de forraje de la leguminosa Desmanthus virgatus L. var. depressus Willd, a diferentes densidades de población y cortes periódicos. Asimismo, se estudió el efecto de la densidad de población en la composición química del forraje. Se utilizó un diseño experimental completamente al azar con arreglo factorial 4 x 4 con cuatro repeticiones. Se evaluaron cuatro densidades de población (3, 4, 6 y 8 plantas/m2 ) y el forraje producido en cuatro cortes consecutivos. En cada corte se cuantificó el rendimiento de materia seca. Al forraje obtenido del tercer corte se le realizó un análisis químico proximal y se determinó el contenido de calcio y fósforo. La interacción cortes con densidad de población fue significativa, ya que el rendimiento de forraje seco en el primero y cuarto cortes aumentó proporcionalmente con la densidad de población (P ≤ 0.05). Sin embargo, en el segundo y tercer cortes el rendimiento fue mayor sólo con la densidad de 6 plantas/m2. Los mayores rendimientos acumulados de los cortes se obtuvieron con densidades de 6 y 8 plantas/m2 . La composición química del forraje del tercer corte fue similar en todas las densidades de población estudiadas.