bluuebunny/crossref_metadata_2025 huggingface dataset:
Optical modulation biosensing system for rapid detection of biological targets at low concentrations
Authors: Margulis Michael , Cohen Meir , Burg Shmuel , Avivi-Mintz Shira , Danielli Amos
Published: July 2021

In many sensitive assays, target molecules are tagged using fluorescently labeled probes and captured using magnetic beads. Here, we introduce an optical modulation biosensing (OMB) system, which aggregates the beads into a small detection area and separates the signal from the background noise by manipulating the laser beam in and out of the cluster of beads. Using the OMB system to detect human interleukin-8, we demonstrated a limit of detection of 0.02 ng/L and a 4-log dynamic range. Using Zika-positive and healthy individuals’ serum samples, we show that the OMB-based Zika IgG serological assay has 96% sensitivity and 100% specificity.
Scanning planar Yagi-Uda antenna for fluorescence detection
Authors: Soltani Navid , Rabbany Esfahany Elham , Druzhinin Sergey I. , Schulte Gregor , Müller Julian , Sledz Florian , Flatae Assegid Mengistu , Butz Benjamin , Schönherr Holger , Markešević Nemanja , Agio Mario
Published: July 2021

An effective approach to improve the detection efficiency of nanoscale light sources relies on a planar antenna configuration, which beams the emitted light into a narrow cone. Planar antennas operate like optical Yagi-Uda antennas, where reflector and director elements are made of metal films. Here we introduce and investigate, both theoretically and experimentally, a scanning implementation of a planar antenna. Using a small ensemble of molecules contained in fluorescent nanobeads placed in the antenna, we independently address the intensity, radiation pattern, and decay rate as a function of distance between a flat-tip scanning gold wire (reflector) and a thin gold film coated on a glass coverslip (director). The scanning planar antenna changes the radiation pattern of a single fluorescent bead, and it beams light into a narrow cone down to angles of45∘(full width at half maximum). Moreover, the collected signal compared to the case of a glass coverslip is larger than a factor of three, which is mainly due to the excitation enhancement. These results offer a better understanding of the modification of light–matter interaction by planar antennas, and they hold promise for applications such as sensing, imaging, and diagnostics.
Terahertz high-Q quasi-bound states in the continuum in laser-fabricated metallic double-slit arrays
Authors: Liu Dejun , Yu Xi , Wu Feng , Xiao Shuyuan , Itoigawa Fumihiro , Ono Shingo
Published: July 2021

A laser-fabricated metallic resonator based on a double-slit array (DSA) is numerically and experimentally demonstrated at terahertz frequencies. Such free-standing resonators achieve a sharp resonance with high quality (Q) factor, arising from a distortion of symmetry-protected bound states in the continuum (BIC). By breaking the structural symmetry of DSAs, the BIC with infinite Q-factor can be transformed into quasi-BICs, and the Q-factors decrease gradually as the asymmetry parameter increases. We analyzed the influence of the imperfection in experimental samples such as the round edge and the trapezoid shape on the transmission properties of DSAs. Different from the DSAs composed of ideal perfect electrical conductors, copper DSAs show lower Q-factor because of the Ohmic loss. The effect of metal thickness on the quasi-BICs for DSAs is also investigated. Results exhibit that thinner resonators can achieve sharper quasi-BICs. These findings suggest that such metallic resonators with high Q-factors have great potential for practical applications in electromagnetic wave filtering and biomolecular sensing.
Orbital angular momentum generator with multiple retroreflection channels enabled by an angle-selective metasurface
Authors: Zhang Zhongtao , Wang Jiafu , Zhu Ruichao , Jia Yuxiang , Yan Mingbao , Qu Shaobo
Published: July 2021

Vortex beams carrying orbital angular momentum (OAM) have aroused great attention on account of the remarkable potential in the field of communication. It has the characteristics of higher spectrum efficiency, greater channel capacity and stronger anti-interference, which will revolutionize the wireless communications in the future. However, target tracking on a vortex generator in practical applications is becoming a challenge because the backscattering of electromagnetic (EM) waves under oblique incidence is too small for detection. Currently, the main way to solve this problem is to load an extra retroreflector such as Luneburg lens, which in turn leads to increased weights and bulky volumes. In this paper, we propose a vortex generator simultaneously with retroreflective characteristics utilizing an angle-selective metasurface. The meta-atom can achieve broadband polarization conversion under normal incidence and efficient retroreflection under oblique incidence. Without the need for an additional retroreflection phase arrangement, an OAM generator composed of such meta-atoms can be achieved in 15.0–21.0GHz under both x- and y-polarized normal incidence. Meanwhile, four retroreflection channels are opened under oblique illumination of both transverse electric (TE) and transverse magnetic (TM) waves at 20.0GHz. Both the simulated and measured results show excellent performances. The integration of an OAM generator and retroreflector will greatly reduce the weight and volume and save in the cost of production, which will promote the development of miniaturized, multi-role, and even intelligent functional devices.
Compressive X-ray tomosynthesis using model-driven deep learning
Authors: Zhao Qile , Ma Xu , Arce Gonzalo R. , Wang Zhiqiang
Published: July 2021

Compressive X-ray tomosynthesis uses a few two-dimensional projection measurements modulated by coding masks to reconstruct the three-dimensional object that can be sparsely represented on a predefined basis. However, the coding mask optimization and object reconstruction require significant computing resources. In addition, existing methods fall short to exploits the synergy between the encoding and reconstruction stages to approach the global optimum. This paper proposes a model-driven deep learning (MDL) approach to significantly improve the computational efficiency and accuracy of tomosynthesis reconstruction. A unified framework is developed to jointly optimize the coding masks and the neural network parameters, which effectively increase the degrees of optimization freedom. It shows that the computational efficiency of coding mask optimization and image reconstruction can be improved by more than one order of magnitude. Furthermore, the performance of reconstruction results is significantly improved.
Fragile topology in double-site honeycomb lattice photonic crystal
Authors: Wei Yizhou , Yan Bei , Peng Yuchen , Shi Aoqian , Zhao Deyun , Peng Rui , Xiang Yuanjiang , Liu Jianjun
Published: July 2021

Fragile topology (FT) opens a new direction in topological photonics, but a new type of photonic crystal (PC) with FT remains to be proposed. In this Letter, the double-site honeycomb lattice (DSHL) PC is proposed by rotating the double dielectric rods (DDR) six times, forming unit cell, and then arraying the unit cells in a triangular lattice. Quantum spin Hall effect occurs by manipulating the DDR in the tangential and radial directions of the unit cell. First, the band structures of DSHL PCs with different structural parameters are calculated, and the laws of topological phase transition are analyzed statistically. Then, to prove the FT properties of two groups of topological nontrivial DSHL PCs, the Wannier-center positions of the bulk bands are calculated by the Wilson–Loop method. Finally, the topological edge states and two groups of topological corner states, which are in the same bulk-state bandgap, are realized successfully. The DSHL PC provides good platforms for both the research of topological photonics and the device design and application, which has a broad prospect.
Inverse spectroscopic optical coherence tomography (IS-OCT) for characterization of particle size and concentration
Authors: Hope James , Goodwin Matthew , Vanholsbeeck Frédérique
Published: July 2021

Inverse spectroscopic optical coherence tomography (IS-OCT) methods apply inverse problem formulations to acquired spectra to estimate depth-resolved sample properties. In the current study, we modelled the time-frequency-distributions using Lambert-Beer’s law and implemented IS-OCT using backscattering spectra calculated from Mie theory, then demonstrated the algorithm on polystyrene microspheres under idealized conditions. The results are significant because the method generates depth dependent estimates of both the concentration and diameter of scattering particles.
Proteasome activity contributes to pro-survival response upon mild mitochondrial stress in Caenorhabditis elegans
Authors: Sladowska Maria , Turek Michał , Kim Min-Ji , Drabikowski Krzysztof , Mussulini Ben Hur Marins , Mohanraj Karthik , Serwa Remigiusz A. , Topf Ulrike , Chacinska Agnieszka
Published: July 2021

Defects in mitochondrial function activate compensatory responses in the cell. Mitochondrial stress that is caused by unfolded proteins inside the organelle induces a transcriptional response (termed the “mitochondrial unfolded protein response” [UPRmt]) that is mediated by activating transcription factor associated with stress 1 (ATFS-1). The UPRmt increases mitochondrial protein quality control. Mitochondrial dysfunction frequently causes defects in the import of proteins, resulting in the accumulation of mitochondrial proteins outside the organelle. In yeast, cells respond to mistargeted mitochondrial proteins by increasing activity of the proteasome in the cytosol (termed the “unfolded protein response activated by mistargeting of proteins” [UPRam]). The presence and relevance of this response in higher eukaryotes is unclear. Here, we demonstrate that defects in mitochondrial protein import in Caenorhabditis elegans lead to proteasome activation and life span extension. Both proteasome activation and life span prolongation partially depend on ATFS-1, despite its lack of influence on proteasomal gene transcription. Importantly, life span prolongation depends on the fully assembled proteasome. Our data provide a link between mitochondrial dysfunction and proteasomal activity and demonstrate its direct relevance to mechanisms that promote longevity.
gen3sis: A general engine for eco-evolutionary simulations of the processes that shape Earth’s biodiversity
Authors: Hagen Oskar , Flück Benjamin , Fopp Fabian , Cabral Juliano S. , Hartig Florian , Pontarp Mikael , Rangel Thiago F. , Pellissier Loïc
Published: July 2021

Understanding the origins of biodiversity has been an aspiration since the days of early naturalists. The immense complexity of ecological, evolutionary, and spatial processes, however, has made this goal elusive to this day. Computer models serve progress in many scientific fields, but in the fields of macroecology and macroevolution, eco-evolutionary models are comparatively less developed. We present a general, spatially explicit, eco-evolutionary engine with a modular implementation that enables the modeling of multiple macroecological and macroevolutionary processes and feedbacks across representative spatiotemporally dynamic landscapes. Modeled processes can include species’ abiotic tolerances, biotic interactions, dispersal, speciation, and evolution of ecological traits. Commonly observed biodiversity patterns, such as α, β, and γ diversity, species ranges, ecological traits, and phylogenies, emerge as simulations proceed. As an illustration, we examine alternative hypotheses expected to have shaped the latitudinal diversity gradient (LDG) during the Earth’s Cenozoic era. Our exploratory simulations simultaneously produce multiple realistic biodiversity patterns, such as the LDG, current species richness, and range size frequencies, as well as phylogenetic metrics. The model engine is open source and available as an R package, enabling future exploration of various landscapes and biological processes, while outputs can be linked with a variety of empirical biodiversity patterns. This work represents a key toward a numeric, interdisciplinary, and mechanistic understanding of the physical and biological processes that shape Earth’s biodiversity.
The super repertoire of type IV effectors in the pangenome of Ehrlichia spp. provides insights into host-specificity and pathogenesis
Authors: Noroy Christophe , Meyer Damien F.
Published: July 2021

The identification of bacterial effectors is essential to understand how obligatory intracellular bacteria such as Ehrlichia spp. manipulate the host cell for survival and replication. Infection of mammals–including humans–by the intracellular pathogenic bacteria Ehrlichia spp. depends largely on the injection of virulence proteins that hijack host cell processes. Several hypothetical virulence proteins have been identified in Ehrlichia spp., but one so far has been experimentally shown to translocate into host cells via the type IV secretion system. However, the current challenge is to identify most of the type IV effectors (T4Es) to fully understand their role in Ehrlichia spp. virulence and host adaptation. Here, we predict the T4E repertoires of four sequenced Ehrlichia spp. and four other Anaplasmataceae as comparative models (pathogenic Anaplasma spp. and Wolbachia endosymbiont) using previously developed S4TE 2.0 software. This analysis identified 579 predicted T4Es (228 pT4Es for Ehrlichia spp. only). The effector repertoires of Ehrlichia spp. overlapped, thereby defining a conserved core effectome of 92 predicted effectors shared by all strains. In addition, 69 species-specific T4Es were predicted with non-canonical GC% mostly in gene sparse regions of the genomes and we observed a bias in pT4Es according to host-specificity. We also identified new protein domain combinations, suggesting novel effector functions. This work presenting the predicted effector collection of Ehrlichia spp. can serve as a guide for future functional characterisation of effectors and design of alternative control strategies against these bacteria.
SCMFMDA: Predicting microRNA-disease associations based on similarity constrained matrix factorization
Authors: Li Lei , Gao Zhen , Wang Yu-Tian , Zhang Ming-Wen , Ni Jian-Cheng , Zheng Chun-Hou , Su Yansen
Published: July 2021

miRNAs belong to small non-coding RNAs that are related to a number of complicated biological processes. Considerable studies have suggested that miRNAs are closely associated with many human diseases. In this study, we proposed a computational model based on Similarity Constrained Matrix Factorization for miRNA-Disease Association Prediction (SCMFMDA). In order to effectively combine different disease and miRNA similarity data, we applied similarity network fusion algorithm to obtain integrated disease similarity (composed of disease functional similarity, disease semantic similarity and disease Gaussian interaction profile kernel similarity) and integrated miRNA similarity (composed of miRNA functional similarity, miRNA sequence similarity and miRNA Gaussian interaction profile kernel similarity). In addition, the L2 regularization terms and similarity constraint terms were added to traditional Nonnegative Matrix Factorization algorithm to predict disease-related miRNAs. SCMFMDA achieved AUCs of 0.9675 and 0.9447 based on global Leave-one-out cross validation and five-fold cross validation, respectively. Furthermore, the case studies on two common human diseases were also implemented to demonstrate the prediction accuracy of SCMFMDA. The out of top 50 predicted miRNAs confirmed by experimental reports that indicated SCMFMDA was effective for prediction of relationship between miRNAs and diseases.
Partial information decomposition reveals that synergistic neural integration is greater downstream of recurrent information flow in organotypic cortical cultures
Authors: Sherrill Samantha P. , Timme Nicholas M. , Beggs John M. , Newman Ehren L.
Published: July 2021

The directionality of network information flow dictates how networks process information. A central component of information processing in both biological and artificial neural networks is their ability to perform synergistic integration–a type of computation. We established previously that synergistic integration varies directly with the strength of feedforward information flow. However, the relationships between both recurrent and feedback information flow and synergistic integration remain unknown. To address this, we analyzed the spiking activity of hundreds of neurons in organotypic cultures of mouse cortex. We asked how empirically observed synergistic integration–determined from partial information decomposition–varied with local functional network structure that was categorized into motifs with varying recurrent and feedback information flow. We found that synergistic integration was elevated in motifs with greater recurrent information flow beyond that expected from the local feedforward information flow. Feedback information flow was interrelated with feedforward information flow and was associated with decreased synergistic integration. Our results indicate that synergistic integration is distinctly influenced by the directionality of local information flow.
Geographical Detector-based influence factors analysis for Echinococcosis prevalence in Tibet, China
Authors: Ma Tian , Jiang Dong , Hao Mengmeng , Fan Peiwei , Zhang Shize , Quzhen Gongsang , Xue ChuiZhao , Han Shuai , Wu WeiPing , Zheng Canjun , Ding Fangyu
Published: July 2021

Echinococcosis, caused by genusEchinococcus, is the most pathogenic zoonotic parasitic disease in the world. In Tibet of the People’s Republic of China, echinococcosis refers principally to two types of severe zoonosis, cystic echinococcosis (CE) and alveolar echinococcosis (AE), which place a serious burden on public health and economy in the local community. However, research on the spatial epidemiology of echinococcosis remains inadequate in Tibet, China. Based on the recorded human echinococcosis data, maps of the spatial distribution of human CE and AE prevalence in Tibet were produced at city level and county level respectively, which show that the prevalence of echinococcosis in northern and western Tibet was much higher than that in other regions. We employ a geographical detector to explore the influencing factors for causing CE and AE while sorting information on the maps of disease prevalence and environment factors (e.g. terrain, population, and yak population). The results of our analysis showed that biological factors have the most impact on the prevalence of echinococcosis, of which the yak population contributes the most for CE, while the dog population contributes the most for AE. In addition, the interaction between various factors, as we found out, might further explain the disease prevalence, which indicated that the echinococcosis prevalence is not simply affected by one single factor, but by multiple factors that are correlated with each other complicatedly. Our results will provide an important reference for the evaluation of the echinococcosis risk, control projects, and prevention programs in Tibet.
Development of the cartilaginous connecting apparatuses in the fetal sphenoid, with a focus on the alar process
Authors: Yamamoto Masahito , Abe Hiroaki , Hirouchi Hidetomo , Sato Masaki , Murakami Gen , Rodríguez-Vázquez José Francisco , Abe Shinichi
Published: July 2021

The human fetal sphenoid is reported to have a cartilaginous connecting apparatus known as the alar process (AP), which connects the ala temporalis (AT) (angle of the greater wing of the sphenoid) to the basisphenoid (anlage of the sphenoid body). However, how the AP develops in humans is unclear. In addition, although the AP is a common structure of the mammalian chondrocranium, little is known about whether it is really a fundamental feature in mammals. This study examined the histological sections of 20 human embryos and fetuses from 6 to 14 weeks of development, of 20 mouse embryos from embryonic days 12–18, and of 4 rats embryos form embryonic days 17 and 20. In addition, we reconsidered the definition of the AP by comparing humans and rats with mice. In humans, the AP was continuous with the basisphenoid but was separated from the AT by a thick perichondrium. Then, the AP–AT connection had a key-and-keyhole structure. Unlike a joint, no cavitation developed in this connection. In mice, there was no boundary between the AT and the basisphenoid, indicating the absence of the AP in the mouse chondrocranium. In rats, the AP was, however, separated from the AT by a thick perichondrium. Therefore, the AP can be defined as follows: the AP is temporally separated from the AT by a thick perichondrium or a key-and-keyhole structure during the fetal period. This is the first study that confirms the absence of the alar process in the mice skull, and its presence in other mammals skull should be further investigated.
Ozone inactivation of airborne influenza and lack of resistance of respiratory syncytial virus to aerosolization and sampling processes
Authors: Dubuis Marie-Eve , Racine Étienne , Vyskocil Jonathan M. , Turgeon Nathalie , Tremblay Christophe , Mukawera Espérance , Boivin Guy , Grandvaux Nathalie , Duchaine Caroline
Published: July 2021

Influenza and RSV are human viruses responsible for outbreaks in hospitals, long-term care facilities and nursing homes. The present study assessed an air treatment using ozone at two relative humidity conditions (RHs) in order to reduce the infectivity of airborne influenza. Bovine pulmonary surfactant (BPS) and synthetic tracheal mucus (STM) were used as aerosols protectants to better reflect the human aerosol composition. Residual ozone concentration inside the aerosol chamber was also measured. RSV’s sensitivity resulted in testing its resistance to aerosolization and sampling processes instead of ozone exposure. The results showed that without supplement and with STM, a reduction in influenza A infectivity of four orders of magnitude was obtained with an exposure to 1.70 ± 0.19 ppm of ozone at 76% RH for 80 min. Consequently, ozone could be considered as a virucidal disinfectant for airborne influenza A. RSV did not withstand the aerosolization and sampling processes required for the use of the experimental setup. Therefore, ozone exposure could not be performed for this virus. Nonetheless, this study provides great insight for the efficacy of ozone as an air treatment for the control of nosocomial influenza A outbreaks.
Epidemiology of Mycobacterium tuberculosis lineages and strain clustering within urban and peri-urban settings in Ethiopia
Authors: Taye Hawult , Alemu Kassahun , Mihret Adane , Ayalew Sosina , Hailu Elena , Wood James L. N. , Shkedy Ziv , Berg Stefan , Aseffa Abraham ,   The ETHICOBOTS consortium
Published: July 2021

Background

Previous work has shown differential predominance of certainMycobacterium tuberculosis (M.tb)lineages and sub-lineages among different human populations in diverse geographic regions of Ethiopia. Nevertheless, how strain diversity is evolving under the ongoing rapid socio-economic and environmental changes is poorly understood. The present study investigated factors associated withM.tblineage predominance and rate of strain clustering within urban and peri-urban settings in Ethiopia.

Methods

Pulmonary Tuberculosis (PTB) and Cervical tuberculous lymphadenitis (TBLN) patients who visited selected health facilities were recruited in the years of 2016 and 2017. A total of 258M.tbisolates identified from 163 sputa and 95 fine-needle aspirates (FNA) were characterized by spoligotyping and compared with internationalM.tbspoligotyping patterns registered at the SITVIT2 databases. The molecular data were linked with clinical and demographic data of the patients for further statistical analysis.

Results

From a total of 258M.tbisolates, 84 distinct spoligotype patterns that included 58 known Shared International Type (SIT) patterns and 26 new or orphan patterns were identified. The majority of strains belonged to two majorM.tblineages, L3 (35.7%) and L4 (61.6%). The observed high percentage of isolates with shared patterns (n = 200/258) suggested a substantial rate of overall clustering (77.5%). After adjusting for the effect of geographical variations, clustering rate was significantly lower among individuals co-infected with HIV and other concomitant chronic disease. Compared to L4, the adjusted odds ratio and 95% confidence interval (AOR; 95% CI) indicated that infections with L3M.tbstrains were more likely to be associated with TBLN [3.47 (1.45, 8.29)] and TB-HIV co-infection [2.84 (1.61, 5.55)].

Conclusion

Despite the observed difference in strain diversity and geographical distribution ofM.tblineages, compared to earlier studies in Ethiopia, the overall rate of strain clustering suggests higher transmission and warrant more detailed investigations into the molecular epidemiology of TB and related factors.
Understanding and predicting COVID-19 clinical trial completion vs. cessation
Authors: Elkin Magdalyn E. , Zhu Xingquan
Published: July 2021

As of March 30 2021, over 5,193 COVID-19 clinical trials have been registered through Clinicaltrial.gov. Among them, 191 trials were terminated, suspended, or withdrawn (indicating the cessation of the study). On the other hand, 909 trials have been completed (indicating the completion of the study). In this study, we propose to study underlying factors of COVID-19 trial completion vs. cessation, and design predictive models to accurately predict whether a COVID-19 trial may complete or cease in the future. We collect 4,441 COVID-19 trials from ClinicalTrial.gov to build a testbed, and design four types of features to characterize clinical trial administration, eligibility, study information, criteria, drug types, study keywords, as well as embedding features commonly used in the state-of-the-art machine learning. Our study shows that drug features and study keywords are most informative features, but all four types of features are essential for accurate trial prediction. By using predictive models, our approach achieves more than 0.87 AUC (Area Under the Curve) score and 0.81 balanced accuracy to correctly predict COVID-19 clinical trial completion vs. cessation. Our research shows that computational methods can deliver effective features to understand difference between completed vs. ceased COVID-19 trials. In addition, such models can also predict COVID-19 trial status with satisfactory accuracy, and help stakeholders better plan trials and minimize costs.
Risk of acute myocardial infarction among new users of chondroitin sulfate: A nested case-control study
Authors: Mazzucchelli Ramón , Rodríguez-Martín Sara , García-Vadillo Alberto , Gil Miguel , Rodríguez-Miguel Antonio , Barreira-Hernández Diana , García-Lledó Alberto , de Abajo Francisco J.
Published: July 2021

Objective

To test the hypothesis that the use of chondroitin sulfate (CS) or glucosamine reduces the risk of acute myocardial infarction (AMI).

Design

Case-control study nested in a primary cohort of patients aged 40 to 99 years, using the database BIFAP during the 2002–2015 study period. From this cohort, we identified incident cases of AMI and randomly selected five controls per case, matched by exact age, gender, and index date. Adjusted odds ratios (AOR) and 95% confidence interval (CI) were computed through a conditional logistic regression. Only new users of CS or glucosamine were considered.

Results

A total of 23,585 incident cases of AMI and 117,405 controls were included. Of them, 89 cases (0.38%) and 757 controls (0.64%) were current users of CS at index date, yielding an AOR of 0.57 (95%CI: 0.46–0.72). The reduced risk among current users was observed in both short-term (<365 days, AOR = 0.58; 95%CI: 0.45–0.75) and long-term users (>364 days AOR = 0.56; 95%CI:0.36–0.87), in both sexes (men, AOR = 0.52; 95%CI:0.38–0.70; women, AOR = 0.65; 95%CI:0.46–0.91), in individuals over or under 70 years of age (AOR = 0.54; 95%CI:0.38–0.77, and AOR = 0.61; 95%CI:0.45–0.82, respectively) and in individuals at intermediate (AOR = 0.65; 95%CI:0.48–0.91) and high cardiovascular risk (AOR = 0.48; 95%CI:0.27–0.83), but not in those at low risk (AOR = 1.11; 95%CI:0.48–2.56). In contrast, the current use of glucosamine was not associated with either increased or decreased risk of AMI (AOR = 0.86; 95%CI:0.66–1.08).

Conclusions

Our results support a cardioprotective effect of CS, while glucosamine seems to be neutral. The protection was remarkable among subgroups at high cardiovascular risk.
Impact of the EU Blue Card programme on cultural participation and subjective well-being of migrants in Germany
Authors: Giovanis Eleftherios , Akdede Sacit Hadi , Ozdamar Oznur
Published: July 2021

The first aim of this study is to investigate the role of the EU Blue Card programme implemented in 2012 in Germany. In particular, we aim to explore the impact on the participation in cultural activities of first-generation non-European Union (EU) and non-European Economic Area (EEA) migrants, such as attendance to cinema, concerts and theatre. The second aim is to examine the impact of cultural activities on subjective well-being (SWB), measured by life satisfaction. We compare the cultural participation and life satisfaction between the treatment group that is the non-EU/EEA first-generation immigrants and the control group that consists, not only of natives and second-generation immigrants but also composes of EU/EEA first-generation immigrants who are not eligible to the programme. We will apply a sharp and a fuzzy regression discontinuity design (RDD) within a seemingly unrelated regression equations (SURE) system using the Ordered Probit method. The empirical analysis relies on data from the German Socio-Economic Panel (GSOEP) survey over the period 2015–2018. The results show that the treated subjects experience an increase in cultural participation activities and an improvement in their SWB, as a result of the EU Blue Card programme, compared to the control group. Participation in classical music performance, opera or theatre influences more the SWB compared to other cultural activities. Policies that promote labour market integration and participation in cultural activities will enable immigrants to integrate into the social norms of the host societies and improve their SWB.
Evaluation of the diagnostic performance of laboratory-based c-reactive protein as a triage test for active pulmonary tuberculosis
Authors: Samuels Thomas H. A. , Wyss Romain , Ongarello Stefano , Moore David A. J. , Schumacher Samuel G. , Denkinger Claudia M.
Published: July 2021

Introduction

A highly sensitive triage test that captures most symptomatic patients at increased likelihood of having pulmonary tuberculosis (PTB) would ‘rule-out’ lower-risk patients from expensive confirmatory testing. Although studies have assessed the diagnostic accuracy of a C-reactive protein (CRP) triage test for PTB in HIV+ patients, limited data are available from HIV- cohorts.

Materials and methods

In this retrospective case-control study, 765 serum samples were selected from FIND’s biobank. Each sample had been collected from an adult presenting with respiratory symptomatology to district hospitals in South Africa and referral hospitals in Cambodia, Peru, Georgia and Vietnam between 2007–2017. Serum CRP measurements were obtained using a laboratory-based assay. CRP cutoff-points of ≥8mg/L and ≥10mg/L were predefined as a positive triage test result. The PTB reference standard was two contemporaneously collected sputum liquid culture results.

Results

CRP demonstrated an overall sensitivity for PTB of 79.8% (95%CI 75.5–83.5) and 77.7% (95%CI 73.4–81.6) for cutoff-points of 8mg/L and 10mg/L respectively. Specificity was 62.8% (95%CI 57.8–67.6%) and 66.6% (95%CI 61.1–70.7) respectively. Area-under-the-curve using Receiver Operating Characteristic analysis was 0.77 (95%CI 0.74–0.81). Threshold analysis showed optimal CRP cutoff-points were higher in HIV+ than HIV- participants. An algorithm in which CRP triage was followed by confirmatory Xpert MTB/Rif testing achieved a sensitivity of 75.1% (95%CI 69.0–80.4%) whilst decreasing Xpert usage by 40.6%.

Discussion

CRP may not meet the challenge of a catch-all TB triage test. However, it shows promise in HIV+ individuals. Further research is required in a prospective study using point-of-care platforms to further evaluate its capabilities.
The effectiveness of eye tracking in the diagnosis of cognitive disorders: A systematic review and meta-analysis
Authors: Liu Zicai , Yang Zhen , Gu Yueming , Liu Huiyu , Wang Pu
Published: July 2021

Background

Eye tracking (ET) is a viable marker for the recognition of cognitive disorders. We assessed the accuracy and clinical value of ET for the diagnosis of cognitive disorders in patients.

Methods

We searched the Medline, Embase, Web of Science, Cochrane Library, and Pubmed databases from inception to March 2, 2021, as well as the reference lists of identified primary studies. We included articles written in English that investigated ET for cognitive disorder patients—Mild cognitive impairment (MCI), Alzheimer’s disease (AD), Amyotrophic lateral sclerosis (ALS), and dementia. Two independent researchers extracted the data and the characteristics of each study; We calculated pooled sensitivities and specificities. A hierarchical summary of receiver performance characteristics (HSROC) model was used to test the diagnostic accuracy of ET for cognitive impairment (CI).

Findings

11 studies met the inclusion criteria and were included in qualitative comprehensive analysis. Meta-analysis was performed on 9 trials using Neuropsychological Cognitive Testing (NCT) as the reference standard. The comprehensive sensitivity and specificity of ET for detecting cognitive disorders were 0.75 (95% CI 0.72–0.79) and 0.73 (95% CI 0.70 to 0.76), respectively. The combined positive likelihood ratio (LR+) was 2.74 (95%CI 2.32–3.24) and the negative likelihood ratio (LR−) was 0.27 (95%CI 0.18–0.42).

Conclusions

This review showed that ET technology could be used to detect the decline in CI, clinical use of ET techniques in combination with other tools to assess CI can be encouraged.
Experiences with using a mobile application for learning evidence-based practice in health and social care education: An interpretive descriptive study
Authors: Johnson Susanne Grødem , Titlestad Kristine Berg , Larun Lillebeth , Ciliska Donna , Olsen Nina Rydland
Published: July 2021

Background

Health and social care students are expected to apply evidence-based practice (EBP). An innovative mobile application, EBPsteps, was developed to support learning EBP.

Aim

The aim of this study was to explore health and social care students’ experiences of learning about EBP using the mobile application EBPsteps during their clinical placements.

Methods

An interpretive description approach guided the exploration of student experiences. Four focus groups were conducted with a convenience sample of students from three undergraduate degree programs: occupational therapy, physical therapy, and social education. The constant comparison method was used to categorize and compare the qualitative data.

Results

Three integrated themes were generated: "triggers for EBP", "barriers to EBP", and "design matters". Information needs, academic requirements, and encouragement from clinical instructors triggered the students to use EBPsteps. Lack of EBP knowledge, lack of academic demand, and lack of emphasis on EBP in clinical placement were barriers to using EBPsteps. Design issues mattered, as use of the app was motivated by design features such as the opportunity to practice EBP in one place and taking notes in a digital notebook. The use of the app was hindered by anticipation that the use of phones during clinical placements would be viewed negatively by others and by specific design features, such as unfamiliar icons.

Conclusions

The students perceived the EBPsteps app as a relevant tool for learning EBP, although they also suggested specific changes to the design of the app. Requirements must be embedded in the curriculum to ensure that the app is used. Our findings bring important information to developing and implementing mobile applications as a teaching method in health and social care educations.
Occurrence of natural enemies of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) in Nigeria
Authors: Ogunfunmilayo Akindele Oluwole , Kazeem Shakiru Adewale , Idoko Joy Ejemen , Adebayo Raphael Abiodun , Fayemi Elizabeth Yetunde , Adedibu Okuyinka Bosola , Oloyede-Kamiyo Qudrah Olaitan , Nwogwugwu Joy Oluchi , Akinbode Oluwafolake Adenike , Salihu Shina , Offord Lisa Clare , Buddie Alan Graham , Ofuya Thomas Inomisan
Published: July 2021

Fall armyworm (FAW; Spodoptera frugiperda), an exotic moth which recently invaded Africa, is a highly destructive pest of cereals especially maize a highly valued staple crop in Nigeria. The use of natural enemies such as predators or parasitoids for FAW control is more economically viable and environmentally safer than currently recommended synthetic insecticides. Natural enemies to combat the pest have not yet been reported in Nigeria. An exploration for the pests’ natural enemies was undertaken by collecting FAW eggs and larvae from maize fields. These were reared in the laboratory for emergence, identification and efficacy as natural enemies. This yielded Euplectrus laphygmae (Hymenoptera: Eulophidae); Telenomus remus (Hymenoptera: Platygastridae) and Trombidium sp. (Acari.: Trombidiidae). Cotesia or Apanteles spp. were inferred to occur since Stictopisthus sp. (Hym.: Ichneumonidae), a secondary parasitoid, that attacks cocoons of Microgasterinae (e.g. Cotesia, Apanteles etc.) also emerged. Species of yet-to-be identified predators were also observed in various niches of maize plants. A positive relationship was found between FAW instar and the number of E. laphygmae eggs/instar ranging, on average, from 1.5 on second instar to 5.5 on fourth instars hosts. Parasitism rate of T. remus on FAW eggs was 100%. Parasitic mite infestation resulted in increasing paleness, reduced feeding, growth and movement as well as death of FAW 1st instars. Thus, the occurrence of FAW natural enemies in Nigeria calls for advocacy campaign to incorporate their use into integrated pest management strategies that attract and allow natural enemies to thrive for FAW management.
A novel olfactory threshold test for screening cognitive decline among elderly people
Authors: Kim Sun Mi , Kim Hye Ri , Min Hyun Jin , Kim Kyung Soo , Jin Jae-Chan , Han Doug Hyun
Published: July 2021

Olfactory impairment is associated with dementia and is a potential early biomarker of cognitive decline. We developed a novel olfactory threshold test called Sniff Bubble using rose odor-containing beads made with 2-phenylethyl alcohol. We aimed to define cut-off scores for this tool to help identify cognitive decline among elderly people. In total, 162 elderly people (mean age ± SD: 73.04 ± 8.73 years) were administered olfactory threshold and neurocognitive tests. For analyses, we divided the participants into two groups based on cognitive functioning, namely cognitive decline (n = 44) and normal cognition (n = 118) groups. The Sniff Bubble and YSK olfactory function test for olfactory threshold and the Structured Clinical Interview for DSM-5 Disorders-Clinician Version and Korean version of the Consortium to Establish a Registry for Alzheimer’s Disease assessment packet for neurocognitive functioning were used. We used K-means cluster analyses and receiver operating characteristic (ROC) analyses to identify the most appropriate cut-off value. We established a positive correlation between the Sniff Bubble and neurocognitive function test scores (r = 0.431, p < 0.001). We defined the cut-off score, using the ROC curve analyses for Sniff Bubble scores, at 3 and higher with an area under the curve of 0.759 (p < 0.001). The Sniff Bubble test can adequately detect cognitive decline in elderly people and may be used clinically as the first step in the screening process.
A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox
Authors: Horikawa Ayumi , Mizuno Keiko , Tsuda Kyoko , Yamamoto Takayoshi , Michiue Tatsuo
Published: July 2021

Human induced pluripotent stem cells (hiPSCs) are considered a promising source of pancreatic β-cells for the treatment of diabetes. However, this approach is limited by issues such as low efficiency and high cost. Here, we have developed a new protocol to induce insulin-producing cells. To reduce costs, we decreased the number of reagents and replaced protein reagents with chemical compounds. In this method, we increased induction efficiency with ascorbic acid (vitamin C) and an ALK5 inhibitor, RepSox. In 2D culture, the majority of cells were immature β-cells with low glucose-stimulated insulin secretion. Transferring to 3D culture immediately after endocrine progenitor cell differentiation, however, improved glucose-stimulated insulin secretion. This simplified method will contribute to realizing transplantation therapy of β-cells using iPSCs.
The effect of acute ventilation-perfusion mismatch on respiratory heat exchange in a porcine model
Authors: Edlinger-Stanger Maximilian , Bernardi Martin-Hermann , Kovacs Katharina , Mascha Michael , Neugebauer Thomas , Böhme Stefan , Ayoubi Nathan , Christofi Nico , Garry James , Fleming Neal , Hiesmayr Michael
Published: July 2021

Background

Respiratory heat exchange is an important physiological process occurring in the upper and lower respiratory tract and is usually completed when inspired gases reach the alveoli. Animal and human studies demonstrated that heat exchange can be modulated by altering pulmonary ventilation and perfusion. The purpose of this study was to examine the effect of acute ventilation-perfusion (V/Q) mismatch on respiratory heat exchange. In clinical practice, monitoring respiratory heat exchange might offer the possibility of real-time tracking of acute V/Q-mismatch.

Methods

In 11 anesthetized, mechanically ventilated pigs, V/Q-mismatch was established by means of four interventions: single lung ventilation, high cardiac output, occlusion of the left pulmonary artery and repeated whole-lung lavage. V/Q-distributions were determined by the multiple inert gas elimination technique (MIGET). Respiratory heat exchange was measured as respiratory enthalpy using the novel, pre-commercial VQm™ monitor (development stage, Rostrum Medical Innovations, Vancouver, CA). According to MIGET, shunt perfusion of low V/Q compartments increased during single lung ventilation, high cardiac output and whole-lung lavage, whereas dead space and ventilation of high V/Q compartments increased during occlusion of the left pulmonary artery and whole-lung lavage.

Results

Bohr dead space increased after pulmonary artery occlusion and whole-lung lavage, venous admixture increased during single lung ventilation and whole-lung lavage, PaO2/FiO2 was decreased during all interventions. MIGET confirmed acute V/Q-mismatch. Respiratory enthalpy did not change significantly despite significant acute V/Q-mismatch.

Conclusion

Clinically relevant V/Q-mismatch does not impair respiratory heat exchange in the absence of additional thermal stressors and may not have clinical utility in the detection of acute changes.
Transcutaneous auricular VNS applied to experimental pain: A paired behavioral and EEG study using thermonociceptive CO2 laser
Authors: Dumoulin Manon , Liberati Giulia , Mouraux André , Santos Susana Ferrao , El Tahry Riëm
Published: July 2021

Background

Transcutaneous auricular Vagal Nerve Stimulation (taVNS) is a non-invasive neurostimulation technique with potential analgesic effects. Several studies based on subjective behavioral responses suggest that taVNS modulates nociception differently with either pro-nociceptive or anti-nociceptive effects.

Objective

This study aimed to characterize how taVNS alters pain perception, by investigating its effects on event-related potentials (ERPs) elicited by different types of spinothalamic and lemniscal somatosensory stimuli, combined with quantitative sensory testing (detection threshold and intensity ratings).

Methods

We performed 3 experiments designed to study the time-dependent effects of taVNS and compare with standard cervical VNS (cVNS). In Experiment 1, we assessed the effects of taVNS after 3 hours of stimulation. In Experiment 2, we focused on the immediate effects of the duty cycle (OFF vs. ON phases). Experiments 1 and 2 included 22 and 15 healthy participants respectively. Both experiments consisted of a 2-day cross-over protocol, in which subjects received taVNS and sham stimulation sequentially. In addition, subjects received a set of nociceptive (thermonociceptive CO2 laser, mechanical pinprick) and non-nociceptive (vibrotactile, cool) stimuli, for which we recorded detection thresholds, intensity of perception and ERPs. Finally, in Experiment 3, we tested 13 epileptic patients with an implanted cVNS by comparing OFF vs. ON cycles, using a similar experimental procedure.

Results

Neither taVNS nor cVNS appeared to modulate the cerebral and behavioral aspects of somatosensory perception.

Conclusion

The potential effect of taVNS on nociception requires a cautious interpretation, as we found no objective change in behavioral and cerebral responses to spinothalamic and lemniscal somatosensory stimulations.
Characterization of a member of the CEACAM protein family as a novel marker of proton pump-rich ionocytes on the zebrafish epidermis
Authors: Kowalewski Julien , Paris Théo , Gonzalez Catherine , Lelièvre Etienne , Castaño Valencia Lina , Boutrois Morgan , Augier Camille , Lutfalla Georges , Yatime Laure
Published: July 2021

In humans, several members of the CEACAM receptor family have been shown to interact with intestinal pathogens in an inflammatory context. While CEACAMs have long been thought to be only present in mammals, recent studies have identified ceacam genes in other vertebrates, including teleosts. The function of these related genes remains however largely unknown. To gain insight into the function of CEACAM proteins in fish, we undertook the study of a putative member of the family, CEACAMz1, identified in Danio rerio. Sequence analysis of the ceacamz1 gene product predicted a GPI-anchored extracellular protein containing eleven immunoglobulin domains but revealed no evident orthology with human CEACAMs. Using a combination of RT-PCR analyses and in situ hybridization experiments, as well as a fluorescent reporter line, we showed that CEACAMz1 is first expressed in discrete cells on the ventral skin of zebrafish larvae and later on in the developing gills. This distribution remains constant until juvenile stage is reached, at which point CEACAMz1 is almost exclusively expressed in gills. We further observed that at late larval stages, CEACAMz1-expressing cells mostly localize on the afferent side of the branchial filaments and possibly in the inter-lamellar space. Using immunolabelling and 3D-reconstructions, we showed that CEACAMz1 is expressed in cells from the uppermost layer of skin epidermis. These cells are embedded within the keratinocytes pavement and we unambiguously identified them as proton-pump rich ionocytes (HR cells). As the expression of ceacamz1 is turned on concomitantly to that of other known markers of HR cells, we propose that ceacamz1 may serve as a novel marker of mature HR cells from the zebrafish epidermis.