bluuebunny/crossref_metadata_2025 huggingface dataset:
Revealing the Mechanism of Protein Degradation in Postmortem Meat: The Role of Phosphorylation and Ubiquitination
Authors: Zhao Xinran , Wu Saisai , Ren Chi , Bai Yuqiang , Hou Chengli , Li Xin , Wang Zhenyu , Zhang Dequan
Published: January 2025

The aim of this study was to investigate the possible effects of phosphorylation and ubiquitination on the degradation of myofibrillar proteins in mutton with different tenderness. The longissimus thoracis lumborum muscles were chosen and divided into tender and tough groups (n = 9), and then stored at 4 °C for 1 h, 12 h, 1 d, 3 d, and 5 d postmortem. Shear force, pH, myofibril fragmentation index, AMPK activity, E3 ubiquitin ligase abundance, protein phosphorylation, and the ubiquitination levels of muscle samples were measured. The results demonstrated that the meat of samples in the tender group had a higher degradation of desmin and a lower phosphorylation level of desmin at 1 d compared with the tough group. The ubiquitination level of desmin, AMPK activity, and E3 ubiquitin ligase abundance in the tender group were noticeably higher than those in the tough group at 12 h. There was a negative correlation between the shear force and desmin degradation. The desmin degradation was negatively correlated with desmin phosphorylation and ubiquitination levels. The phosphorylation level of desmin was positively correlated with its ubiquitination. In summary, this study suggests that AMPK and E3 ubiquitin ligase concurrently play significant roles in regulating meat tenderness by regulating phosphorylation and ubiquitination in meat postmortem.
Fit for the Future: An Assessment of the Sustainability Parameters of Liquid Dairy Product Packaging in the DACH Region and the Implications of Upcoming Regulatory Changes
Authors: Klein Michelle , Neumair Charlotte , Primoceri Mattia , Tacker Manfred , Apprich Silvia
Published: January 2025

The European Union aims for climate neutrality by 2050 and has proposed the Packaging and Packing Waste Regulation (PPWR) to promote a circular economy, focusing on reducing packaging waste. In this context, a comprehensive sustainability assessment for liquid dairy product packaging, including beverage cartons, bottles and to-go cups, in the DACH region (Germany, Austria and Switzerland) was conducted. The aim was to consider various ecological aspects of environmental impacts and circularity. As the aspect of recyclability is a core aspect in the PPWR, the calculation was of central interest in this project. Here, major differences in the waste management infrastructure between countries could be identified. The majority of assessed packaging falls below the PPWR’s 70% recyclability requirement, with Switzerland showing even lower recyclability due to poor packaging collection and recycling infrastructure. Significant discrepancies in packaging efficiency exist, indicating unnecessary resource consumption, especially in the case of to-go cups. Additionally, the carbon footprint of packaging materials can vary up to ten times within certain product categories, negatively impacting the environment. Good results were identified for the use of certified renewable resources. Overall, the results of the assessment demonstrate several areas for improvement in light of forthcoming regulatory requirements, which must be met in Germany and Austria.
N-Tuple Network Search in Othello Using Genetic Algorithms
Authors: Kuramitsu Hiroto , Suzuki Kaiyu , Matsuzawa Tomofumi
Published: January 2025

As one of the strongest Othello agents, Edax employs an n-tuple network to evaluate the board, with points of interest represented as tuples. However, this network maintains a constant shape throughout the game, whereas the points of interest in Othello vary with respect to game’s progress. The present study was conducted to optimize the shape of the n-tuple network using a genetic algorithm to maximize final score prediction accuracy for a certain number of moves. We selected shapes for 18-, 22-, 26-, 30-, 34-, 38-, 42-, and 46-move configurations, and constructed an agent that appropriately shapes an n-tuple network depending on the progress of the game. Consequently, agents using the n-tuple network developed in this study exhibited a winning rate of 75%. This method is independent of game characteristics and can optimize the shape of larger (or smaller) N-tuple networks.
Relationship Between Thermal Conductivity, Mineral Composition and Major Element Composition in Rocks from Central and South Germany
Authors: Suft Oliver , Hagenauer Hannes , Bertermann David
Published: January 2025

Thermal conductivity is a decisive parameter in all geothermal applications. In addition to the influencing factors of density, saturation, porosity, temperature and pressure, it is, above all, the geochemical and mineralogical composition that determines the thermal conductivity in rocks and soils. This study focuses on selected rock samples from Southern and Central Germany regarding major element oxides and minerals as well as distributed thermal conductivity. We examined clastic and chemical sedimentary, as well as igneous and metamorphic rocks, ranging from the Paleozoic to Cenozoic age. Measurements were conducted by X-ray fluorescence analysis (XRF), X-ray diffraction (XRD) and optical scanning with a thermal conductivity scanner (TCS). The results show significant correlations between thermal and geochemical parameters. Chemical composition significantly impacts thermal conductivity. Higher quartz and SiO2 contents generally lead to increased thermal conductivity, while aluminum silicates, common in clay minerals, correlate with lower conductivity. For carbonates, increased density or reduced porosity enhances conductivity. Structural differences and differing mineral concentrations influence the measurement variability along the sampling axis. This is especially visible in clastic sedimentary rock samples, where porosity decreases while cementation of the matrix increases thermal conductivity.
Factors Predicting Sexual Satisfaction of Thai Women with Cervical Cancer After Complete Treatment at Least One Year and Their Spouses
Authors: Bualoy Wunwisa , Sumdaengrit Bualuang , Junda Tiraporn , Lertkhachonsuk Arb-aroon
Published: January 2025

Background: Sexual satisfaction is vital for the sexual health and well-being of both cervical cancer patients and their spouses. Sexual dissatisfaction can arise from negative treatment, making it important to examine the factors that influence sexual satisfaction. Objective: The purpose of this study was to explore the factors affecting the sexual satisfaction of Thai women with cervical cancer after complete treatment and their spouses. Materials and Methods: A predictive correlational study was conducted with 100 couples using convenience sampling. The study was based on the ecological theory framework. Data were collected from April 2023 to January 2024 in two tertiary hospitals through five questionnaires: a demographic questionnaire, the Thai Hospital Anxiety and Depression Scale, the Charlson Comorbidity Index, the Couple Relationship Scale, Natsal 2000, and the New Sexual Satisfaction Scale. Data analysis was performed using multiple linear regression. Results: The multiple regression analysis showed that sexual difficulties and duration of marriage predicted 25.6% of the variance in sexual satisfaction among women with cervical cancer. The duration of marriage (b = −0.48, p = 0.012) had a stronger impact than sexual difficulties (b = −2.82, p = 0.005). Sexual difficulties and couple relationships accounted for 34.9% of the variance in sexual satisfaction for spouses. Sexual difficulties (b = −3.13, p = 0.004) had a greater effect than couple relationships (b = 0.30, p = 0.003). Conclusions: Healthcare providers should promote constructive communication between couples through open, respectful, and supportive dialogue to strengthen their relationship, improve mutual understanding, address sexual difficulties, and enhance sexual satisfaction for women with cervical cancer after treatment and their spouses. The findings results can inform future intervention research aimed at improving sexual satisfaction in this population one-year post treatment.
Current Practices and Perceived Effectiveness of Clinicians Regarding Polynucleotide Injection for Knee Osteoarthritis: A Survey-Based Evaluation
Authors: Lee Dagyeong , Kim Wan-ho , Ha Jeong Han , Kim Hyungjin , Kim Junbae , Shin Dong Wook
Published: January 2025

Aims: Intra-articular (IA) injection therapy, particularly IA hyaluronic acid (HA), is a common treatment for knee osteoarthritis, but it does have limitations. The injection of IA polynucleotide (PN) has emerged as an alternative, potentially offering superior clinical outcomes. This study investigates current practice patterns and the perceived effectiveness of PN among clinicians for treating knee osteoarthritis in the Republic of Korea. Methods: Based on a survey conducted among clinicians who use PN in clinical practice, we explored the current practices and assessed the perceived effectiveness of IA PN in treating knee osteoarthritis. Results: A total of 265 clinicians who used IA PN for knee osteoarthritis participated in the survey. Most clinicians (73.3%) used PN therapy for the treatment of chronic pain, with varying administration frequencies. In addition, 25.8% of clinicians used PN for the treatment of acute flare-ups. In cases of knee effusion, 55.5% of clinicians removed the effusion prior to administering PN. Clinicians rated PN as more effective than HA for both chronic pain and acute flare-ups, with higher scores for cushioning, anti-inflammatory effects, and delaying joint degeneration. The clinicians stated that patients expressed a higher satisfaction with IA PN compared with IA HA, noting improvement in joint smoothness, noise reduction, pain relief, and a reduction in heat sensation and swelling. Conclusions: The results of the present study highlight the extensive use and perceived benefits among clinicians of IA PN for knee osteoarthritis in the Republic of Korea. Further research is warranted to explore the effectiveness of PN in acute flare-ups and to validate these findings in broader populations.
Safety and Satisfaction Analysis of Intravenous and Inhalational Conscious Sedation in a Geriatric Population Undergoing Oral Surgery
Authors: Iglesias-Rodeiro Elizabeth , Ruiz-Sáenz Pedro Luis , Madrigal Martínez-Pereda Cristina , Barona-Dorado Cristina , Fernández-Cáliz Fernando , Martínez-Rodríguez Natalia
Published: January 2025

Aim: The objective of this research was to compare two methods of conscious sedation (midazolam vs. sevoflurane) used for performing oral surgical procedures in the older adult population by analyzing dental treatment-related anxiety levels, the quality of sedation, and potential hemodynamic changes during the interventions, as well as post-recovery symptoms and patient satisfaction levels. Methods: A total of 104 patients underwent oral surgery with intravenous (age: 84.00 ± 8.410; 17 men and 36 women) and inhalation conscious sedation (age: 82.73 ± 8.809; 18 men and 33 women). Anxiety levels were recorded using the Modified Corah Dental Anxiety Scale (MDAS) and the Hamilton Anxiety Rating Scale (HARS). During the intervention, the heart rate, systolic/diastolic blood pressure, oxygen saturation, episodes of hypoxia (<90%SpO2), sedation duration, and Sedation–Agitation Scale (SAS) values were monitored. Upon completion of their treatment, all patients completed three questionnaires which evaluated their recall of the intervention, postoperative symptoms, and recommendation of the sedation method used. Results: Anxiety levels were slightly higher in the midazolam group (MDAS score: 9.60 ± 5.849; HARS score: 27.19 ± 6.802) compared to the sevoflurane group (MDAS score: 8.37 ± 5.063; HARS score: 24.92 ± 5.199), with no statistical significance (MDAS, p = 0.453/HARS, p = 0.065). No significant differences in the analyzed hemodynamic parameters were observed between the two groups. However, SAS values were significantly higher in the sevoflurane group (p = 0.006), while the sedation duration was longer in the midazolam group (p = 0.000). Postoperative symptoms, particularly gastrointestinal disturbances and dry mouth, were significantly more prevalent in the midazolam group, while there were no differences in neurological symptoms between the two groups. The majority of patients expressed a preference for either of these sedation methods. Conclusions: Sedation with agents such as midazolam and sevoflurane appears to be quite safe, given the absence of relevant hemodynamic changes. Midazolam has been shown to be effective for a longer duration, as well as to have a lower risk of developing greater digestive problems during the postoperative period. On the contrary, sevoflurane produced a greater sensation of dry mouth. Both sedative agents are perceived by the older adult population as satisfactory, recommending their use.
The Health Effects of Karate Training: A Review of 21st Century Research
Authors: Piepiora Paweł Adam
Published: January 2025

Background/Objectives: To date, the health effects of karate have not been identified. Therefore, the aim of this article is to learn more about the health effects of karate training based on a review of current research. Methods: The Scopus database was searched from 2000 onwards for available articles related only to karate. The following intermediate phrases were not included: combat sport, fighting art, hand-to-hand combat, martial art, and self-defence system. The filter used was desk review analysis. Only 14 scientific articles (research papers and review papers) strictly on karate were found. The method of source material analysis and critical analysis of the source text was then used. Results: It was shown that kumite appears to require significantly more metabolic power than kata. Furthermore, the greater skill of karatekas is associated with their greater physical fitness, and long-term karate training attenuates the decline in dynamic visual acuity (DVA). The role of modified and individualised forms of karate training is also highlighted as important for the health of practitioners. Furthermore, long-term karate practice is associated with extensive modulation of immunological parameters. Karate training can also significantly improve motor skills. It can also play an important role in the development of willpower and personality traits that contribute to the well-being of its practitioners. Furthermore, nutritional and psychological interventions combined with karate training may improve cardiometabolic parameters, oxidative stress and inflammation. Karate training may also contribute to the prevention of osteoporosis and strengthen the left ventricular myocardium. Conclusions: It was found that there is a paucity of contemporary research on the health effects of karate training. In addition, they are limited to the individuals studied, so that generalisations about these effects in the general population of karate practitioners cannot be made.
The Mediating Effect of Social Networks on the Impact of Health Perceptions on the Quality of Life in Older Adults
Authors: Durmuş Sarıkahya Selma , Terzi Amine , Kanbay Yalçın , Çınar Özbay Sevil
Published: January 2025

Background/Objective: Social networks help improve psychosocial and quality-of-life outcomes among older adults. This study aimed to examine the mediating role of social networks in the effect of health perception on the quality of life of the elderly. Methods: The sample of the study consisted of 327 people over the age of 65 who applied to a family health center. The study data were collected using the “Personal Information Form”, “Perception of Health Scale”, “World Health Organization Quality of Life Instrument-Older Adults Module”, and “Lubben Social Network Scale”. Multivariate regression analyses and mediation effect examinations were conducted to explore the relationships between social networks, health perception, and quality-of-life outcomes. Results: According to the findings, social networks mediate the relationship between health perception and quality of life. Individuals with a high level of health perception and a high level of social networks have higher quality-of-life levels than others. Conclusions: The results of the study confirm the significant correlation between health perception and the quality of life, as well as any potential links between these factors and social networks that affect older people’s quality of life.
Occupational Burnout in Nurses and Corporate Employees in Małopolska Region, Poland
Authors: Nowacka Anna , Gniadek Agnieszka , Micek Agnieszka , Świątek Paulina , Wadas Tadeusz , Wolfshaut-Wolak Renata
Published: January 2025

Introduction: Work is an essential aspect of human life. However, high expectations from employers and clients, combined with time pressure and chronic stress, can contribute to burnout among employees in service professions. Aim of the Study: This study aimed to compare the prevalence of burnout syndrome between two occupational groups—corporate office workers and active nurses—and to assess the influence of socio-demographic factors on the level of burnout in both groups. Materials and Methods: The study was conducted among 330 participants, including 126 nurses (38%) and 204 corporate employees (62%). Data were collected using a standard questionnaire and the Maslach Burnout Inventory. The study period spanned from November 2018 to October 2019, and questionnaires were distributed via email. Results: The findings revealed that both corporate employees and nurses experience occupational burnout. Both groups predominantly exhibited moderate to high levels of emotional exhaustion (nurses: 66.67%, q2 = 20.5; corporate employees: 73.53%, q2 = 31.5) and low to moderate levels of personal accomplishment (nurses: 78.57%, q2 = 32.0; corporate employees: 87.75%, q2 = 27.0). Corporate employees showed significantly higher depersonalization scores (q2 = 13.50) compared to nurses (q2 = 5.0). The prevalence of burnout in both groups was influenced by socio-demographic factors, including having children, their place of residence, education, earnings, and job-related factors such as vacation availability, the frequency of work shifts, work systems, overtime, and overall job satisfaction. Conclusions: Service-oriented professions that involve caregiving and direct interpersonal interactions face comparable risks of occupational burnout. Despite differences in the nature and conditions of work, as well as tasks performed, both corporate employees and active nurses are vulnerable to burnout. High salaries were not confirmed as a protective factor against occupational burnout. Additionally, environmental factors, particularly those related to working conditions, played a significant role in the development of burnout syndrome, alongside individual factors.
The Effects of Yoga on Fall-Related Physical Functions for Older Women: A Systematic Review of Randomized Controlled Trials
Authors: Huang Tzu-Chun , Li Ching , Hsieh Ching-Yu
Published: January 2025

Introduction: The evidence showed that the risk of falls was higher in women, and yoga was considered an effective rehabilitation method for preventing falls. However, there had been no previous attempts to synthesize the evidence specifically for the use of yoga in preventing falls among older women. Objectives: This systematic review aimed to strengthen the existing body of evidence by focusing exclusively on the impact of yoga in improving fall-related physical functions among older women. Methods: A systematic review was conducted following the PRISMA guidelines. The protocol was developed in advance of the study and registered on PROSPERO (Registration number: CRD42024506550). Results: The effects of yoga on balance, gait, and lower-limb strength were inconsistent. It showed that yoga programs designed to prevent falls in older women might not demonstrate the same effectiveness as those identified in previous systematic reviews for the general older adult population. Conclusions: This systematic review is the first to exclusively explore the impact of yoga on fall-related physical functions in older women. However, this review did not directly observe a definitive effect of yoga on fall prevention in older women. Future studies should delve deeper into identifying appropriate yoga postures and determining the optimal dose required to enhance physical function and prevent falls.
Effect of Plant Growth-Promoting Rhizobacteria Synthetic Consortium on Growth, Yield, and Metabolic Profile of Lettuce (Lactuca sativa L.) Grown Under Suboptimal Nutrient Regime
Authors: Abou Jaoudé Renée , Luziatelli Francesca , Ficca Anna Grazia , Ruzzi Maurizio
Published: January 2025

Soilless cultivation allows for the exploitation of the benefits of plant growth-promoting rhizobacteria (PGPR) without the loss of efficacy observed with soil inoculation. In this study, we investigated the effects of a PGPR consortium on the plant growth, ecophysiology, and metabolic profile of lettuce (Lactuca sativa L.) grown in an aeroponic system under a low-nutrient regime. Overall, the plant biomass increased by 25% in the PGPR-inoculated plants due to enhanced leaf and root growth. The rise in the leaf biomass was primarily due to an increase in the leaf number and average leaf mass, coupled with a higher total leaf area. In addition, the inoculated plants exhibited an altered leaf anatomy characterized by an increased palisade parenchyma thickness and reduced airspace area, suggesting an improved photosynthetic efficiency and changes in the mesophyll conductance. The root morphology was also altered, with the PGPR-inoculated plants showing higher lateral root development. Furthermore, PGPR inoculation induced significant metabolic reprogramming in the leaves, affecting several pathways related to growth, development, and stress responses. These findings provide valuable insights into the intricate metabolic dialog between plants and beneficial microbes and demonstrate that the integration of soilless culture with an analysis of the ecophysiological, anatomical, and metabolomic plant responses can be a powerful approach to accelerate the design of new PGPR consortia for use as microbial biostimulants.
Treatment of Onychomycosis and the Drug–Drug Interactions in Patients with Diabetes Mellitus and Diabetic Foot Syndrome: A Systematic Review
Authors: Navarro-Pérez David , Tardáguila-García Aroa , García-Oreja Sara , Álvaro-Afonso Francisco Javier , López-Moral Mateo , Lázaro-Martínez José Luis
Published: January 2025

Background: This systematic review reports on treatments for onychomycosis in patients with diabetes and the drug interactions with other drugs in regard to the complicated diabetic patient profile. Methods: The recommendations in the preferred reporting items for systematic reviews and meta-analysis (PRISMA) checklist were applied and the included studies were evaluated using the Consolidated Standards of Reporting Trials (CONSORT) statement and the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement. Searches were conducted in November 2023, using the PubMed (Medline), Scopus, Cochrane Library, and Web of Science databases; studies on antifungal treatments for onychomycosis in patients with diabetes were included. Two authors performed the study selection and data extraction, and any discrepancies between the two reviewers were resolved through discussions with a third reviewer. This review was registered in PROSPERO (CRD42023442107). Results: The systematic review included 10 studies that met the selection criteria. Mycological cures for mild to moderate onychomycosis were: Ageratina pichinchensis (8.6%), 8% ciclopirox (8.6% 24 weeks and 54.3% 48 weeks), 10% efinaconazole (56.5–58.33%), terbinafine (73–76.6%), itraconazole (88.2%), and laser therapy (43.8%). No serious adverse effects or drug interactions were observed because patients with major complications, such as peripheral vascular disease, diabetic neuropathy, liver and renal dysfunction, poorly controlled diabetes, and severe onychomycosis, were excluded. Conclusions: The antifungal treatments described in the included studies are safe for patients with well-controlled diabetes, but there are currently no studies involving patients with diabetes and multiple complications, such as diabetic foot syndrome or severe onychomycosis. Thus, further research is needed in terms of this patient profile.
Community Training Institute for Health Disparities: Outcomes of a Formal Opportunity for Community Capacity Building to Increase Health Equity in Southern Puerto Rico
Authors: Jiménez-Chávez Julio , Rosario-Maldonado Fernando J. , Vélez-Maldonado David A. , Rodríguez-Torruella Dorimar , Aguirre-Hernández Jeannie M. , Castro-Figueroa Eida , Asencio-Toro Gloria , Rivera-Mateo Elizabeth , Morales-Torres Luisa , Ramos-Lucca Axel , Motta-Pagán Jorge L. , Wallerstein Nina , Marzán-Rodríguez Melissa
Published: January 2025

Integration of the community into health research through community-engaged research has proven to be an essential strategy for reducing health inequities. It brings significant benefits by addressing community health concerns and promoting active community participation in research. The Community Training Institute for Health Disparities (CTIHD) was established to support this integration based on Community-Based Participatory Research (CBPR) principles. The main objective of this paper is to report the CTIHD program’s implementation, evaluation, and outcomes from the first two cohorts. The CTIHD recruited Hispanic community members (N = 54) to be trained in health disparities research and health promotion to foster Community–Academic Partnerships (CAPs) and develop community-led health promotion interventions. Evaluation measures included satisfaction, knowledge change, retention rate, completion rate, and project proposals (research and community health promotion plans). The retention and completion rates were 83% and 78%, respectively, with forty-two (n = 42) community trainees receiving the completion certification. Both cohorts demonstrated a significant increase in knowledge (p < 0.05), and overall satisfaction exceeded 90%. Outcomes include seven (7) community–academic partnerships, leading to the co-development of research proposals, three (3) of which received funding. Additionally, twenty-two (22) community health promotion plans were developed, with seven (7) implemented, impacting 224 individuals. Findings from this study suggest that the CTIHD effectively provided capacity building, promoted the formation of CAPs, and increased community-led health promotion interventions, thereby advancing health disparity research and community initiatives.
Onvansertib and Navitoclax Combination as a New Therapeutic Option for Mucinous Ovarian Carcinoma
Authors: Petrella Serena , Colombo Marika , Marabese Mirko , Grasselli Chiara , Panfili Andrea , Chiappa Michela , Sancisi Valentina , Craparotta Ilaria , Barbera Maria C. , Cassanmagnago Giada A. , Bolis Marco , Damia Giovanna
Published: January 2025

Mucinous epithelial ovarian cancer (mEOC) is a rare subtype of epithelial ovarian cancer, characterized by poor responses to standard platinum-based chemotherapy. Polo-like kinase 1 (PLK1) is a key regulator of mitosis and cell cycle progression and its inhibition has been recently identified as a target in mEOC. In this study, we aimed to identify further therapeutic targets in mEOC using a CRISPR/Cas9 library targeting 3015 genes, with and without treatment with onvansertib, a PLK1 inhibitor. We identified twelve genes associated with cell survival (ZC2HC1C, RPA2, KIN17, TUBG1, SMC2, CDC26, CDC42, HOXA9, TAF10, SENP1, MRPS31, and COPS2) and three genes (JUND, CARD9, and BCL2L2) in synthetic lethality with onvansertib treatment. We validated that SENP1 downregulation is important for the growth of mEOC cells through esiRNA interference and the use of a pharmacological inhibitor Momordin Ic. The downregulation of CARD9 and BCL2L2 combined with subtoxic doses of onvansertib interfered with mEOC cell growth. Interestingly, the combination of navitoclax, an inhibitor of BcL2 family members including BCL2L2, was synergistic in all four of the mEOC cell lines tested and substantially induced cell death through apoptosis. These data support the use of a combination of navitoclax and onvansertib as a new therapeutic strategy for mEOC.
Cr-Doped Nanocrystalline TiO2-Cr2O3 Nanocomposites with p-p Heterojunction as a Stable Gas-Sensitive Material
Authors: Kuranov Dmitriy , Konstantinova Elizaveta , Grebenkina Anastasia , Sagitova Alina , Platonov Vadim , Polomoshnov Sergei , Rumyantseva Marina , Krivetskiy Valeriy
Published: January 2025

Nanocrystalline TiO2 is a perspective semiconductor gas-sensing material due to its long-term stability of performance, but it is limited in application because of high electrical resistance. In this paper, a gas-sensing nanocomposite material with p-p heterojunction is introduced based on p-conducting Cr-doped TiO2 in combination with p-conducting Cr2O3. Materials were synthesized via a single-step flame spray pyrolysis (FSP) technique and comprehensively studied by X-ray diffraction (XRD), Brunauer–Emmett–Teller (BET) specific surface area analysis, transition electron microscopy (TEM), energy dispersive X-ray (EDX) spectroscopy, X-ray photoelectron spectroscopy (XPS), electron paramagnetic resonance (EPR), and Raman spectroscopy. Gas sensor performance in direct current (DC) mode was studied toward a number of gasses (H2, CO, CH4, NO2, H2S, NH3) as well as volatile organic compounds (VOCs) (acetone, methanol, and formaldehyde) in dry and humid conditions. The long-term stability of the obtained materials’ gas sensor performance was evaluated alongside with an ex situ study of structural evolution. High sensitivity toward oxygenated VOCs and a lower detection limit below ppm level with a limited influence of humidity were shown. The long-term gas sensor performance stability of the obtained materials and its connection to the defect structure of doped TiO2 is demonstrated.
Lessons from Deep Learning Structural Prediction of Multistate Multidomain Proteins—The Case Study of Coiled-Coil NOD-like Receptors
Authors: Șulea Teodor Asvadur , Martin Eliza Cristina , Bugeac Cosmin Alexandru , Bectaș Floriana Sibel , Iacob Anca-L , Spiridon Laurențiu , Petrescu Andrei-Jose
Published: January 2025

We test here the prediction capabilities of the new generation of deep learning predictors in the more challenging situation of multistate multidomain proteins by using as a case study a coiled-coil family of Nucleotide-binding Oligomerization Domain-like (NOD-like) receptors from A. thaliana and a few extra examples for reference. Results reveal a truly remarkable ability of these platforms to correctly predict the 3D structure of modules that fold in well-established topologies. A lower performance is noticed in modeling morphing regions of these proteins, such as the coiled coils. Predictors also display a good sensitivity to local sequence drifts upon the modeling solution of the overall modular configuration. In multivalued 1D to 3D mappings, the platforms display a marked tendency to model proteins in the most compact configuration and must be retrained by information filtering to drive modeling toward the sparser ones. Bias toward order and compactness is seen at the secondary structure level as well. All in all, using AI predictors for modeling multidomain multistate proteins when global templates are at hand is fruitful, but the above challenges have to be taken into account. In the absence of global templates, a piecewise modeling approach with experimentally constrained reconstruction of the global architecture might give more realistic results.
Enhancing Radiation Therapy Response in Prostate Cancer Through Metabolic Modulation by Mito-Lonidamine: A 1H and 31P Magnetic Resonance Spectroscopy Study
Authors: Orlovskiy Stepan , Gupta Pradeep Kumar , Arias-Mendoza Fernando , Singh Dinesh Kumar , Nova Skyler , Nelson David S. , Narayan Vivek , Koch Cameron J. , Hardy Micael , You Ming , Kalyanaraman Balaraman , Nath Kavindra
Published: January 2025

Radiation therapy (RT) is the cornerstone treatment for prostate cancer; however, it frequently induces gastrointestinal and genitourinary toxicities that substantially diminish the patients’ quality of life. While many individuals experience transient side effects, a subset endures persistent, long-term complications. A promising strategy to mitigate these toxicities involves enhancing tumor radiosensitivity, potentially allowing for lower radiation doses. In this context, mito-lonidamine (Mito-LND), an antineoplastic agent targeting the mitochondrial electron transport chain’s complexes I and II, emerges as a potential radiosensitizer. This study investigated Mito-LND’s capacity to augment RT efficacy and reduce adverse effects through comprehensive in vitro and in vivo assessments using hormone-sensitive and hormone-refractory prostate cancer models. Employing a Seahorse analysis and 1H/31P magnetic resonance spectroscopy (MRS), we observed that Mito-LND selectively suppressed lactate production, decreased intracellular pH, and reduced bioenergetics and oxygen consumption levels within tumor cells. These findings suggest that Mito-LND remodels the tumor microenvironment by inducing acidification, metabolic de-energization, and enhanced oxygenation, thereby sensitizing tumors to RT. Our results underscore the potential of Mito-LND as a therapeutic adjunct in RT to improve patient outcomes and reduce radiation-associated toxicities in early-stage prostate cancer.
Integration of ATAC-Seq and RNA-Seq Reveals the Role of FaTIP1 in Red Light-Induced Fruit Ripening in Strawberry
Authors: Chen Xiaodong , Cai Weijian , Xia Jin , Wang Jing , Yuan Huazhao , Wang Qinglian , Pang Fuhua , Zhao Mizhen , Qiao Yushan
Published: January 2025

Light is an important environmental factor affecting the ripening and quality of strawberry fruit. Previous studies have shown that red light treatment can promote strawberry ripening. Gene expression is closely associated with chromatin openness, and changes in chromatin accessibility are crucial for the binding of transcription factors to downstream regulatory sequences. However, the changes in chromatin accessibility in response to different light treatments in octoploid strawberry plants are still unclear. In this study, the landscape of chromatin accessibility of octoploid strawberry under red (R) and yellow–green (YG) light conditions was analyzed by the assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq). Through bioinformatics and Venn diagram analyses, a total of 1456 and 1854 group-specific genes (GSGs) were screened in the R and YG groups, respectively. By using RNA sequencing (RNA-seq), 440 differentially expressed genes (DEGs) were identified. Among these genes, 194 were upregulated under red light treatment. Through joint analysis of ATAC-seq and RNA-seq data, three red group-specific genes with increased expression were identified, namely, FaTIP1, FaQKY and FaLBD1. Through gene expression and transient transformation analyses of strawberry fruit, we further demonstrated that FaTIP1 can respond to red light induction and promote the ripening process of strawberry fruit. Our results provide a reference for the study of chromatin accessibility in octoploid strawberry and reveal new factors involved in the fruit’s response to red light and the regulation of the ripening process of strawberry fruit.
Comprehensive Identification of AREB Gene Family in Populus euphratica Oliv. and Functional Analysis of PeAREB04 in Drought Tolerance
Authors: Liu Binglei , Sun Jianhao , Qiu Chen , Han Xiaoli , Li Zhijun
Published: January 2025

The transcription factors in the ABA Response Element Binding (AREB) protein family were differentially regulated under multiple stress conditions; however, functional analyses of AREB in Populus euphratica Oliv. had not been conducted previously. In the present study, the comprehensive identification of the P. euphratica AREB gene family and the function of PeAREB04 in response to drought stress in P. euphratica were elucidated. A comprehensive analysis of the PeAREB family was first performed, followed by the determination of their expression patterns under drought stress. Bioinformatics analysis revealed that thirteen AREB genes were identified across the P. euphratica genome, with these genes distributed across eight chromosomes in a seemingly random pattern. Phylogenetic analysis indicated that the PeAREB genes could be categorized into four distinct branches. Cis-acting element analysis revealed that most PeAREB genes contained multiple hormone- and stress-responsive elements. Transcriptomic sequencing of P. euphratica seedlings under drought stress showed that most PeAREB genes responded rapidly to drought stress in either the leaves or roots. One gene, PeAREB04, was selected for further functional validation due to its significant upregulation in both leaves and roots under drought stress. Overexpression of PeAREB04 in Arabidopsis thaliana resulted in a high survival rate, reduced water loss in isolated leaves, and a significant reduction in stomatal aperture under natural drought conditions. Drought stress simulations using mannitol further demonstrated that overexpression of PeAREB04 significantly enhanced root elongation. These findings indicate that the identification of the PeAREB gene family and the characterization of PeAREB04’s role in drought stress have been largely accomplished. Furthermore, the PeAREB04 gene demonstrates considerable potential as a key target for future genetic engineering strategies aimed at enhancing plant drought resistance.
The Involvement of Glutamate-mGluR5 Signaling in the Development of Vulvar Hypersensitivity
Authors: Awad-Igbaria Yaseen , Abu-Ata Saher , Sakas Reem , Bang Sarina , Fishboom Tom , Shamir Alon , Bornstein Jacob , Lowenstein Lior , Palzur Eilam
Published: January 2025

Provoked vulvodynia (PV) is the leading cause of vulvar pain and dyspareunia. The etiology of PV is multifactorial and remains poorly understood. PV is associated with a history of repeated vulvar inflammation and is often accompanied by sensory neuromodulation as a result of activation of the metabotropic glutamate receptor 5 (mGluR5) in the sensory nerve terminals. Therefore, this study aims to examine the role of glutamate-mGluR5 signaling during the initial inflammatory phase in chronic vulvar pain development in an animal model of PV.Thermal and mechanical vulvar sensitivity was assessed for three weeks following zymosan vulvar challenges. Anxiety-like behavior and locomotor activity were assessed at the end of the experiment. To investigate the role of glutamate mGluR5, the MTEP (mGluR5 antagonist) was injected into the vulva during vulvar inflammation. On the other hand, glutamate or CHPG (mGluR5 agonist) were injected in order to examine the effects of mGluR5 activation. RT-PCR was performed to assess changes in the transcription of genes related to neuroinflammation, neuromodulation, and neuroplasticity in the spinal cord (L6-S3). Zymosan-induced inflammation resulted in a significant thermal and mechanical vulvar hypersensitivity that persisted for over a month after the zymosan injection. However, local treatment with MTEP enhanced the vulvar mechanical and thermal hypersensitivity. On the other hand, activation of the mGluR5 via injection of glutamate or CHPG into the vulva leads to long-lasting vulvar mechanical and thermal hypersensitivity. The activation of the glutamate pathway was found to be accompanied by an increase in the transcription level of genes related to neuroinflammation and neuroplasticity in the sacral spine region. The present findings indicate that vulvar hypersensitivity is mediated by mGluR5 activation during inflammation. Hence, modulation of the mGluR5 pathway during the critical period of inflammation contributes to preventing chronic vulvar pain development. Conversely, activation of the mGluR5 pathway leads to long-lasting mechanical and thermal hypersensitivity.
OAS1: A Protective Mechanism for Alzheimer’s Disease? An Exploration of Data and Possible Mechanisms
Authors: Elsworthy Richard J. , Pearce Alex , Masoudzadeh Farnoush , Koska Klaudia , Lodhiya Honey , Meher Gargi , Adjej Jodelle , Brookes Keeley J.
Published: January 2025

The immune system and neuroinflammation are now well established in the aetiology of neurodegeneration. Previous studies of transcriptomic and gene association studies have highlighted the potential of the 2′–5′ oligoadenylate synthetase 1 (OAS1) to play a role in Alzheimer’s disease. OAS1 is a viral response gene, interferon-induced, dsRNA activated enzyme, which binds RNase L to degrade dsRNA, and has been associated with COVID-19 response. This study explores whether a viral defence gene could play a vital role in neurodegeneration pathology. The genotyping of five SNPs across the OAS1 locus was conducted in the Brains for Dementia Research (BDR) Cohort for association with AD. RNA-sequencing data were explored for differences in OAS1 gene expression between phenotypes and genotypes. Finally, levels of dsRNA were measured in control cell lines, prior to and after exposure to amyloid oligomers and in cells harbouring a dementia-relevant mutation. No association of any of the OAS1 SNPs investigated were associated with the AD phenotype in the BDR cohort. However, gene expression data supported the previous observation that the minor allele haplotype was associated with higher levels of the OAS1 gene expression and the presence of an alternative transcript. Further to this, the presence of endogenous dsRNA was found to increase with exposure to amyloid oligomers and in the cell line with a dementia-relevant mutation. The data presented here suggest further exploration of the OAS1 gene in relation to dementia is warranted. Investigations of whether carriers of the protective OAS1 haplotype lower dsRNA presence and in turn lower inflammation and cell death are required to support the role of the gene as a moderator of neurodegeneration.
Computational Design of an Energy-Efficient Small Axial-Flow Fan Using Staggered Blades with Winglets
Authors: Tutar Mustafa , Cam Janset Betul
Published: January 2025

The present study introduces a conceptual design of a small axial-flow fan. Both individual and combined effects of blade stagger angle and winglet on the performance of the fan design are investigated in design and off-design operating conditions using a computational flow methodology. A stepwise solution, in which a proper stagger angle adjustment of a specifically generated blade profile is followed by appending a winglet at the tip of the blade with consideration of different geometrical parameters, is proposed to improve the performance characteristics of the fan. The initial model comparison analysis demonstrates that a three-dimensional, Reynolds-averaged Navier–Stokes (RANS) equation-based renormalization group (RNG) k–ε turbulence modeling approach coupled with the multiple reference frame (MRF) technique which adapts multi-block topology generation meshing method successfully resolves the rotating flow around the fan. The results suggest that the use of a proper stagger angle with the winglet considerably increases the fan performance and the fan attains the best total efficiency with an additional stagger angle of +10° and a winglet, which has a curvature radius of 6.77 mm and a twist angle of −7° for the investigated dimensioning range. The present study also underlines the effectiveness of passive flow control mechanisms of the stagger angle and winglets for energy-efficient axial-flow fans.
Supporting Knowledge Transfer on Functional Significance of Forest Biodiversity
Authors: Alfred Radl , Harald Vacik
Published: January 2025

The FunDivEurope (Functional Significance of Forest Biodiversity in Europe) project aimed to quantify the role of forest biodiversity for ecosystem functioning and the delivery of goods and services in major European forest types. Members of the research community aimed to communicate the research findings related to the functional significance of forest biodiversity to the wider public. Therefore, a web-based Knowledge Transfer Platform (KTP) was designed to ensure project-generated knowledge is transferred to targeted stakeholders and user groups. The paper shows a user experience-based approach in the development of the knowledge transfer platform, and provides insights into the system architecture to show how semantic web-based technologies are able to target a broader audience while keeping entry barriers as low as possible to support communities of practice to grow.
The Impact of Production Techniques on Pore Size Distribution in High-Strength Foam Concrete
Authors: Markin Slava , Sahmenko Genadijs , Korjakins Aleksandrs , Mechtcherine Viktor
Published: January 2025

This study examined the impact of various foam concrete production techniques on pore size distribution and its water absorption properties. Techniques such as the use of a cavitation disintegrator and a turbulent mixer were employed to produce foam concrete. Six foam concrete compositions, with dry densities ranging from 820 to 1480 kg/m3 and compressive strength up to 47 MPa, were prepared. A novel method for digital image correlation was applied to analyse the pore size distribution within the foam concrete specimens. The manufactured foam concrete specimens’ porosity and water absorption indices were determined. The experimental results, including compression strength and water absorption, indicated that the production technique significantly affects the pore size distribution in foam concrete, impacting its mechanical and durability properties. Compressive strength was assessed at curing intervals of 7, 28, and 180 days. Cavitation technology was found to promote the formation of a finer porous structure in foam concrete, resulting in enhanced strength properties.
Laboratory Exploration of Several Potential Biocontrol Methods Against the Ambrosia Beetle, Euwallacea interjectus
Authors: Liu Jialin , Jiang Nan , Gao Haiming , Lai Shengchang , Zhou Yang , Hao Dejun , Dai Lulu
Published: January 2025

The ambrosia beetle Euwallacea interjectus Blandford (Coleoptera: Curculionidae: Scolytinae) has recently emerged as a pest in Chinese poplar plantations, causing significant economic losses through damage to host trees in association with its mutualistic fungus Fusarium populicola. This study evaluated the biocontrol potential of Beauveria bassiana strain B-BB-1, Serratia marcescens strain B-SM-1, its metabolite prodigiosin, and two ectoparasitic mites, Pyemotes moseri and Pyemotes zhonghuajia. B. bassiana exhibited significant lethality toward adult female E. interjectus, reduced offspring production, and inhibited F. populicola growth. S. marcescens and prodigiosin had certain lethal effects on larvae and inhibited the growth of F. populicola. Both mites effectively parasitized beetle pupae and larvae, with no significant differences in efficacy between the two species. These agents demonstrate promise for the biological control of E. interjectus, offering insights for managing ambrosia beetle infestations.