bluuebunny/crossref_metadata_2025 huggingface dataset:
CRISP-R/Cas9 Mediated Deletion of Copper Transport Genes CTR1 and DMT1 in NSCLC Cell Line H1299. Biological and Pharmacological Consequences
Authors: Ilyechova Ekaterina Y. , Bonaldi Elisa , Orlov Iurii A. , Skomorokhova Ekaterina A. , Puchkova Ludmila V. , Broggini Massimo
Published: April 2019

Copper, the highly toxic micronutrient, plays two essential roles: it is a catalytic and structural cofactor for Cu-dependent enzymes, and it acts as a secondary messenger. In the cells, copper is imported by CTR1 (high-affinity copper transporter 1), a transmembrane high-affinity copper importer, and DMT1 (divalent metal transporter). In cytosol, enzyme-specific chaperones receive copper from CTR1 C-terminus and deliver it to their apoenzymes. DMT1 cannot be a donor of catalytic copper because it does not have a cytosol domain which is required for copper transfer to the Cu-chaperons that assist the formation of cuproenzymes. Here, we assume that DMT1 can mediate copper way required for a regulatory copper pool. To verify this hypothesis, we used CRISPR/Cas9 to generate H1299 cell line with CTR1 or DMT1 single knockout (KO) and CTR1/DMT1 double knockout (DKO). To confirm KOs of the genes qRT-PCR were used. Two independent clones for each gene were selected for further studies. In CTR1 KO cells, expression of the DMT1 gene was significantly increased and vice versa. In subcellular compartments of the derived cells, copper concentration dropped, however, in nuclei basal level of copper did not change dramatically. CTR1 KO cells, but not DMT1 KO, demonstrated reduced sensitivity to cisplatin and silver ions, the agents that enter the cell through CTR1. Using single CTR1 and DMT1 KO, we were able to show that both, CTR1 and DMT1, provided the formation of vital intracellular cuproenzymes (SOD1, COX), but not secretory ceruloplasmin. The loss of CTR1 resulted in a decrease in the level of COMMD1, XIAP, and NF-κB. Differently, the DMT1 deficiency induced increase of the COMMD1, HIF1α, and XIAP levels. The possibility of using CTR1 KO and DMT1 KO cells to study homeodynamics of catalytic and signaling copper selectively is discussed.
The Potential Use of Metformin, Dipyridamole, N-Acetylcysteine and Statins as Adjunctive Therapy for Systemic Lupus Erythematosus
Authors: Tan Marcus Kai Xuan , Heng Thurston Yan Jia , Mak Anselm
Published: April 2019

Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune condition that can potentially affect every single organ during the course of the disease, leading to increased morbidity and mortality, and reduced health-related quality of life. While curative treatment is currently non-existent for SLE, therapeutic agents such as glucocorticoids, mycophenolate, azathioprine, cyclosporine, cyclophosphamide and various biologics are the mainstay of treatment based on their immunomodulatory and immunosuppressive properties. As a result of global immunosuppression, the side-effect profile of the current therapeutic approach is unfavourable, with adverse effects including myelosuppression, infection and malignancies. Hydroxychloroquine, one of the very few Food and Drug Administration (FDA)-approved medications for the treatment of SLE, has been shown to offer a number of therapeutic benefits to SLE patients independent of its immunomodulatory effect. As such, it is worth exploring drugs similar to hydroxychloroquine that confer additional clinical benefits unrelated to immunosuppressive mechanisms. Indeed, apart from hydroxychloroquine, a number of studies have explored the use of a few conventionally non-immunosuppressive drugs that are potentially useful in the management of SLE. In this review, non-immunosuppressive therapeutic agents, namely metformin, dipyridamole, N-acetylcysteine and statins, will be critically discussed with regard to their mechanisms of action and efficacy pertaining to their potential therapeutic role in SLE.
High Glucose Concentrations Negatively Regulate the IGF1R/Src/ERK Axis through the MicroRNA-9 in Colorectal Cancer
Authors: Chen Ya-Chun , Ou Ming-Che , Fang Chia-Wei , Lee Tsung-Hsien , Tzeng Shu-Ling
Published: April 2019

Studies have revealed that people with hyperglycemia have a high risk of colorectal cancer (CRC). Hyperglycemia may be responsible for supplying energy to CRC cells. However, the potential molecular mechanism for this association remains unclear. Furthermore, microRNA-9 (miR-9) has a tumor-suppressive function in CRC. Aberrant reduced expression of miR-9 is involved in the development and progression of malignancy caused by a high glucose (HG) concentration. In this study, we used an HG concentration to activate miR-9 downregulation in CRC cells. Our results indicated that miR-9 decreased the insulin-like growth factor-1 receptor (IGF1R)/Src signaling pathway and downstream cyclin B1 and N-cadherin but upregulated E-cadherin. The HG concentration not only promoted cell proliferation, increased the G1 population, and modulated epithelial-to-mesenchymal transition (EMT) protein expression and morphology but also promoted the cell migration and invasion ability of SW480 (low metastatic potential) and SW620 (high metastatic potential) cells. In addition, low glucose concentrations could reverse the effect of the HG concentration in SW480 and SW620 cells. In conclusion, our results provide new evidence for multiple signaling pathways being regulated through hyperglycemia in CRC. We propose that blood sugar control may serve as a potential strategy for the clinical management of CRC.
Generation of GLA-Knockout Human Embryonic Stem Cell Lines to Model Autophagic Dysfunction and Exosome Secretion in Fabry Disease-Associated Hypertrophic Cardiomyopathy
Authors: Song Hui-Yung , Chien Chian-Shiu , Yarmishyn Aliaksandr A. , Chou Shih-Jie , Yang Yi-Ping , Wang Mong-Lien , Wang Chien-Ying , Leu Hsin-Bang , Yu Wen-Chung , Chang Yuh-Lih , Chiou Shih-Hwa
Published: April 2019

Fabry disease (FD) is a rare inherited disorder characterized by a wide range of systemic symptoms; it is particularly associated with cardiovascular and renal problems. Enzyme replacement therapy and pharmacological chaperone migalastat are the only approved and effective treatment strategies for FD patients. It is well documented that alpha-galactosidase A (GLA) enzyme activity deficiency causes globotriaosylceramide (Gb3) accumulation, which plays a crucial role in the etiology of FD. However, the detailed mechanisms remain unclear, and the lack of a reliable and powerful disease model is an obstacle. In this study, we created such a model by using CRISPR/Cas9-mediated editing of GLA gene to knockout its expression in human embryonic stem cells (hESCs). The cardiomyocytes differentiated from these hESCs (GLA-null CMs) were characterized by the accumulation of Gb3 and significant increases of cell surface area, the landmarks of FD-associated cardiomyopathy. Furthermore, we used mass spectrometry to compare the proteomes of GLA-null CMs and parental wild type CMs and found that the Rab GTPases involved in exocytotic vesicle release were significantly downregulated. This caused impairment of autophagic flux and protein turnover, resulting in an increase of reactive oxygen species and apoptosis. To summarize, we established a FD model which can be used as a promising tool to study human hypertrophic cardiomyopathy in a physiologically and pathologically relevant manner and to develop new therapies by targeting Rab GTPases signaling-related exosomal vesicles transportation.
Neuroprotection, Recovery of Function and Endogenous Neurogenesis in Traumatic Spinal Cord Injury Following Transplantation of Activated Adipose Tissue
Authors: Carelli Stephana , Giallongo Toniella , Rey Federica , Colli Mattia , Tosi Delfina , Bulfamante Gaetano , Di Giulio Anna Maria , Gorio Alfredo
Published: April 2019

Spinal cord injury (SCI) is a devastating disease, which leads to paralysis and is associated to substantially high costs for the individual and society. At present, no effective therapies are available. Here, the use of mechanically-activated lipoaspirate adipose tissue (MALS) in a murine experimental model of SCI is presented. Our results show that, following acute intraspinal MALS transplantation, there is an engraftment at injury site with the acute powerful inhibition of the posttraumatic inflammatory response, followed by a significant progressive improvement in recovery of function. This is accompanied by spinal cord tissue preservation at the lesion site with the promotion of endogenous neurogenesis as indicated by the significant increase of Nestin-positive cells in perilesional areas. Cells originated from MALS infiltrate profoundly the recipient cord, while the extra-dural fat transplant is gradually impoverished in stromal cells. Altogether, these novel results suggest the potential of MALS application in the promotion of recovery in SCI.
Toolkit for Population Health Initiatives Around the Globe Related to Collaborative Comprehensive Medication Management for Children and Youth
Authors: Parrish Richard , van den Anker Johannes
Published: April 2019

Almost 30 million babies worldwide are born prematurely or become ill annually and need specialized care to survive. Formalized collaborative practice agreements (CPA) between clinical pharmacists and physicians have been put forward as a means for improving the overall medicating experience in many patient populations, including children. This report briefly describes opportunities for collaboration using examples from countries on each continent where CPA is established in professional governance documents and standards. It also provides resources in the form of a toolkit for countries and pharmacist–physician collaborators to authorize and form CPAs to provide comprehensive medication management (CMM) for children and youth with special health care needs (CSHCN).
Study of the Lower Stratospheric Temperature over the Arabian Peninsula
Authors: M. Hasanean Hosny , H. Labban Abdulhaleem
Published: April 2019

Given the current concerns about climate change, particularly, the stratospheric temperature effect on climatic surface temperature, it is of fundamental importance to investigate the impact of the lower stratospheric temperature on regional climate. This paper examines the lower stratospheric temperature (LST) at 50 hPa in the winter season and its relation to the surface air temperature (ST) over the Arabian Peninsula in the period 1951–2016. Generally, LST had an insignificant decreasing trend over the entire period, with a relatively high standard deviation of 1.3 °C. According to the coefficient of variability (COV), the fluctuation in the LST from year to year is relatively high, especially in the last reference period (1981–2010). An insignificant increasing (0.05 °C per year) trend through the period between 1951 and 1992 was observed, while an insignificant decreasing (−0.05 °C per year) trend was observed during the second period of 1993–2016. From the spectral analysis, the periodicity cycles of LST time series at periods of about 2.2, 2.54, and 13.2 years with respect to the 95% significance confidence level were found. LST may be influenced by quasi-biennial oscillation and the sunspot cycle. A strong relationship was found between LST and ST over the Arabian Peninsula.
Broadband High-Reflection Dielectric PVD Coating with Low Stress and High Adhesion on PMMA
Authors: Li Zizheng , Li Qiang , Quan Xiangqian , Zhang Xin , Song Chi , Yang Haigui , Wang Xiaoyi , Gao Jinsong
Published: April 2019

Polymethyl methacrylate (PMMA) is an attractive optical plastic that is widely used in augmented reality, virtual reality devices, display, wearable devices, portable optical equipment, and lightweight optics. Thin film prepared by physical vapor deposition (PVD) is the primary choice of coating on PMMA. However, it faces problems with coating adhesion and stress. In this paper, we analyze the problems existing in the current PMMA high-reflection (HR) coating in detail and propose a way to effectively solve issues with bonding force and stress. Based on the current research background, the bonding force was enhanced by introducing a special hard coating as the connection layer between the dielectric film and the substrate. After comparing the stresses of different coating materials and material combinations, the optimal combination of Nb2O5 and SiO2 was determined, and the requirements were successfully prepared. An HR coating that satisfies requirements, with low stress and excellent environmental adaptability, was successfully prepared. Based on this, a broadband HR coating from 750 to 1550 nm was formed on the surface of PMMA by adjusting the partial pressure of oxygen.
Crystal Growth of Quantum Magnets in the Rare-Earth Pyrosilicate Family R2Si2O7 (R = Yb, Er) Using the Optical Floating Zone Method
Authors: Nair Harikrishnan S. , DeLazzer Tim , Reeder Tim , Sikorski Antony , Hester Gavin , Ross Kate A.
Published: April 2019

We report on the crystal growth of rare-earth pyrosilicates, R 2Si 2O 7 for R = Yb and Er using the optical floating zone method. The grown crystals comprise members from the family of pyrosilicates where the rare-earth atoms form a distorted honeycomb lattice. C-Yb 2Si 2O 7 is a quantum dimer magnet with field-induced long range magnetic order, while D-Er 2Si 2O 7 is an Ising-type antiferromagnet. Both growths resulted in multi-crystal boules, with cracks forming between the different crystal orientations. The Yb 2Si 2O 7 crystals form the C-type rare-earth pyrosilicate structure with space group C 2 / m and are colorless and transparent or milky white, whereas the Er-variant is D-type, P 2 1 / b , and has a pink hue originating from Er 3 +. The crystal structures of the grown single crystals were confirmed through a Rietveld analysis of the powder X-ray diffraction patterns from pulverized crystals. The specific heat of both C-Yb 2Si 2O 7 and D-Er 2Si 2O 7 measured down to 50 mK indicated a phase transition at T N ≈ 1.8 K for D-Er 2Si 2O 7 and a broad Schottky-type feature with a sharp anomaly at 250 mK in an applied magnetic field of 0.8T along the c-axis in the case of C-Yb 2Si 2O 7 .
Temperature Dependence of Raman Frequency Shift in SrWO4 Crystal Studied by Lattice Dynamical Calculations
Authors: Suda Jun , Zverev Petr G.
Published: April 2019

The frequency shift of the Raman modes in strontium tungstate (SrWO4) was investigated in the temperature range from 15 to 295 K. The experimental temperature dependence of the shift was analyzed using both the lattice dynamical calculations and the lattice perturbative approach. We found that the quartic anharmonic term of the first-order perturbation and the cubic term of the second-order perturbation, as well as the thermal expansion, contribute to the temperature shift of the highest-frequency Ag(ν1) mode. The values of the temperature sensitivity of the frequency shift of the Raman modes at room temperature were measured, which is important for developing high-power crystalline Raman lasers and frequency shifters.
Flexible Photonic Nanojet Formed by Cylindrical Graded-Index Lens
Authors: Liu Cheng-Yang
Published: April 2019

Photonic nanojets formed in the vicinity of the cylindrical graded-index lens with different types of index grading are numerically investigated based on the finite-difference time-domain method. The cylindrical lens with 1600 nm diameter is assembled by eighty-seven hexagonally arranged close-contact nanofibers with 160 nm diameter. Simulation and analysis results show that it is possible to engineer and elongate the photonic nanojet. Using differently graded-index nanofibers as building elements to compose this lens, the latitudinal and longitudinal sizes of the produced photonic nanojet can be flexibly adjusted. At an incident wavelength of 532 nm, the cylindrical lens with index grading = 2 can generate a photonic nanojet with a waist about 173 nm (0.32 wavelength). This lens could potentially contribute to the development of a novel device for breaking the diffraction limit in the field of optical nano-scope and bio-photonics.
Testing the Poleotolerance Lichen Response Trait as an Indicator of Anthropic Disturbance in an Urban Environment
Authors: Rocha Bernardo , Pinho Pedro , Vieira Joana , Branquinho Cristina , Matos Paula
Published: April 2019

Urban environments are densely populated areas buzzing with a wide range of anthropic activities that cause disturbances like air pollution or the heat island effect, threatening both human and environmental health. Mitigating its impacts implies understanding the integrated effects that those disturbances exert on urban environments. Lichen biodiversity is frequently used as an ecological indicator, being able to integrate its effects in a quantifiable way. The poleotolerance response trait classifies lichens according to their tolerance to human disturbance, but it was developed for Italy’s flora and has seldom been applied outside Italy or in urban context studies. The aim of this work was to assess this trait suitability as an indicator of urban anthropic disturbance and test it outside Italy. For that, we sampled lichen diversity in 41 green spaces in Lisbon. Lichens were classified into the respective poleotolerance trait functional groups and their community weighted mean related with three type of environmental variables used as surrogates of urban disturbance. We showed that disturbance-tolerant functional groups could be used as an ecological indicator of the integrated effects of environmental disturbances. Some species were clearly misclassified, so we propose reclassification for those. Natural and seminatural functional groups did not behave as expected. Nevertheless, disturbance-tolerant functional groups have the potential to be used in in other Southern European cities.
Bayesian Network Modelling of ATC Complexity Metrics for Future SESAR Demand and Capacity Balance Solutions
Authors: Gomez Comendador Victor Fernando , Arnaldo Valdés Rosa Maria , Villegas Diaz Manuel , Puntero Parla Eva , Zheng Danlin
Published: April 2019

Demand & Capacity Management solutions are key SESAR (Single European Sky ATM Research) research projects to adapt future airspace to the expected high air traffic growth in a Trajectory Based Operations (TBO) environment. These solutions rely on processes, methods and metrics regarding the complexity assessment of traffic flows. However, current complexity methodologies and metrics do not properly take into account the impact of trajectories’ uncertainty to the quality of complexity predictions of air traffic demand. This paper proposes the development of several Bayesian network (BN) models to identify the impacts of TBO uncertainties to the quality of the predictions of complexity of air traffic demand for two particular Demand Capacity Balance (DCB) solutions developed by SESAR 2020, i.e., Dynamic Airspace Configuration (DAC) and Flight Centric Air Traffic Control (FCA). In total, seven BN models are elicited covering each concept at different time horizons. The models allow evaluating the influence of the “complexity generators” in the “complexity metrics”. Moreover, when the required level for the uncertainty of complexity is set, the networks allow identifying by how much uncertainty of the input variables should improve.
The Impact of Financial Constraints on the Convertible Bond Announcement Returns
Authors: Chang Chong-Chuo , Kam Tai-Yung , Chien Chih-Chung , Su Wan Ting
Published: April 2019

As of now, very few research studies have examined the effects of financial constraints on the short- and long-term performances of companies after their announcement of convertible bonds. Due to asymmetric information, previous studies consider issuance of convertible bonds as negative news. As a result, the short- and long-term performances of companies generally decline after their convertible bond announcement. This study argues that when companies have investment plans, they are expected to have higher future cash flows. They will become increasingly more valuable regardless of the fact that they raise funds through the issue of convertible bonds (due to financial constraints), positively affecting the performance of companies. The results indicate that financial constraints have no effect on short-term performance, but did have a significantly positive impact on the long-term performance of companies after their issuance of convertible bonds.
Simulation of AlGaN/GaN HEMTs’ Breakdown Voltage Enhancement Using Gate Field-Plate, Source Field-Plate and Drain Field Plate
Authors: Liao Biyan , Zhou Quanbin , Qin Jian , Wang Hong
Published: April 2019

A 2-D simulation of off-state breakdown voltage (VBD) for AlGaN/GaN high electron mobility transistors (HEMTs) with multi field-plates (FPs) is presented in this paper. The effect of geometrical variables of FP and insulator layer on electric field distribution and VBD are investigated systematically. The FPs can modulate the potential lines and distribution of an electric field, and the insulator layer would influence the modulation effect of FPs. In addition, we designed a structure of HEMT which simultaneously contains gate FP, source FP and drain FP. It is found that the VBD of AlGaN/GaN HEMTs can be improved greatly with the corporation of gate FP, source FP and drain FP. We achieved the highest VBD in the HEMT contained with three FPs by optimizing the structural parameters including length of FPs, thickness of FPs, and insulator layer. For HEMT with three FPs, FP-S alleviates the concentration of the electric field more effectively. When the length of the source FP is 24 μm and the insulator thickness between the FP-S and the AlGaN surface is 1950 nm, corresponding to the average electric field of about 3 MV/cm at the channel, VBD reaches 2200 V. More importantly, the 2D simulation model is based on a real HMET device and will provide guidance for the design of a practical device.
Aerial Surveillance with Low-Altitude Long-Endurance Tethered Multirotor UAVs Using Photovoltaic Power Management System
Authors: Jung Sunghun , Jo Yonghyeon , Kim Young-Joon
Published: April 2019

For a continuous surveillance mission using a swarm of multiple tethered low-altitude long-endurance (LALE) multirotor-type unmanned aerial vehicles (UAVs), we developed a 500 W class photovoltaic power management system (PPMS) which monitors voltage and current flows of photovoltaic (PV) panels, battery pack, and UAV and controls power flows to support UAV flight operation. In contract to a fixed-wing UAV, a tethered multirotor UAV can generate continuously varying closed-circuit television (CCTV) like ground map images by stitching incoming images though the operation range is limited. With an indoor flight experiment, we demonstrated the usefulness of the PPMS and proved operation integrity. According to the results, a total of six multirotor UAVs were required to continuously perform a surveillance mission for 5 h 46 min from 11:04 to 16:50.
The Evaluation of Outdoor Thermal Sensation and Outdoor Energy Efficiency of a Commercial Pedestrianized Zone
Authors: Ma Xuan , Fukuda Hiroatsu , Zhou Dian , Wang Mengying
Published: April 2019

The growth of the scale of cities intensifies urban heat island (UHI) by obstructing the wind and building more radiation at pedestrian level, thus leading to an energy consumption. Commercial pedestrianized-zones cannot only become symbols of cities but also an important factor increasing local economic income. This study conducts on-site measurement and numerical simulation to evaluate the cooling energy efficiency of different parameters (building, vegetation, pavement material) in Fo Shan city, which locates in hot-summer and warm-winter climate region of China. Also, calculations are done to evaluate the index physiological equivalent temperature (PET) for understanding thermal sensation at a pedestrian level (1.5 m). To evaluate different impacts of this zone renewal on the environment and choose the most energy-saving method, it is easy for us to utilize the linear regression for understanding the relationship between coverage ratio of trees (TCR) and thermal comfort in canyon space, which shows that ∆PET = 0.1703 × TCR + 0.2444 with a most important R2 value of 0.9836, for TCR increases from 12.5% to 22%. In open space, also increasing coverage ratio of trees (TCR) can effectively improve humans’ thermal comfort, which shows that ∆PET = 0.2644 × TCR + 0.3955 with a most important R2 value of 0.8892.
Development of a Computational System to Improve Wind Farm Layout, Part II: Wind Turbine Wakes Interaction
Authors: Rodrigues Rafael V. , Lengsfeld Corinne
Published: April 2019

The second part of this work describes a wind turbine Computational Fluid Dynamics (CFD) simulation capable of modeling wake effects. The work is intended to establish a computational framework from which to investigate wind farm layout. Following the first part of this work that described the near wake flow field, the physical domain of the validated model in the near wake was adapted and extended to include the far wake. Additionally, the numerical approach implemented allowed to efficiently model the effects of the wake interaction between rows in a wind farm with reduced computational costs. The influence of some wind farm design parameters on the wake development was assessed: Tip Speed Ratio (TSR), free-stream velocity, and pitch angle. The results showed that the velocity and turbulence intensity profiles in the far wake are dependent on the TSR. The wake profile did not present significant sensitivity to the pitch angle for values kept close to the designed condition. The capability of the proposed CFD model showed to be consistent when compared with field data and kinematical models results, presenting similar ranges of wake deficit. In conclusion, the computational models proposed in this work can be used to improve wind farm layout considering wake effects.
Epidemic Spreading in Urban Areas Using Agent-Based Transportation Models
Authors: Hackl Jürgen , Dubernet Thibaut
Published: April 2019

Human mobility is a key element in the understanding of epidemic spreading. Thus, correctly modeling and quantifying human mobility is critical for studying large-scale spatial transmission of infectious diseases and improving epidemic control. In this study, a large-scale agent-based transport simulation (MATSim) is linked with a generic epidemic spread model to simulate the spread of communicable diseases in an urban environment. The use of an agent-based model allows reproduction of the real-world behavior of individuals’ daily path in an urban setting and allows the capture of interactions among them, in the form of a spatial-temporal social network. This model is used to study seasonal influenza outbreaks in the metropolitan area of Zurich, Switzerland. The observations of the agent-based models are compared with results from classical SIR models. The model presented is a prototype that can be used to analyze multiple scenarios in the case of a disease spread at an urban scale, considering variations of different model parameters settings. The results of this simulation can help to improve comprehension of the disease spread dynamics and to take better steps towards the prevention and control of an epidemic.
A Smart Cities LoRaWAN Network Based on Autonomous Base Stations (BS) for Some Countries with Limited Internet Access
Authors: Barro Pape Abdoulaye , Zennaro Marco , Degila Jules , Pietrosemoli Ermanno
Published: April 2019

An increasing number of implementations of IoT for development use the LoRaWAN protocol as many of them leverage the free network and application servers provided by The Things Networks (TTN) to fulfill their needs. Unfortunately, in some countries in Sub-Saharan Africa and South Asia, Internet access cannot be taken for granted, therefore, TTN might not be available. Moreover, low-cost and low-power consumption options devices are the most sustainable ones. In this paper, we propose a LoRaWAN network with autonomous base stations that can work without Internet connectivity for essential services, while being able to provide additional features whenever Internet access becomes available, even in an intermittent fashion. Security and privacy are preserved, with support for mobile nodes.
Influence of Age on Stress Responses of White Seabream to Amyloodiniosis
Authors: Moreira Márcio , Cordeiro-Silva Anaísa , Barata Marisa , Pousão-Ferreira Pedro , Soares Florbela
Published: April 2019

Amyloodiniosis is a disease that represents a major bottleneck for semi-intensive aquaculture, especially in Southern Europe. The inefficacy of many of the treatments for this disease on marine fish produced in semi-intensive aquaculture has led to a new welfare approach to amyloodiniosis. There is already some knowledge of several welfare issues that lead to amyloodiniosis as well as the stress, physiological, and immunological responses to the parasite by the host, but no work is available about the influence of fish age on the progression of amyloodiniosis. The objective of this work was to determine if stress, hematological, and histopathological responses are age dependent. For that purpose, we determined the mortality rate, histopathological lesions, hematological indexes, and stress responses (cortisol, glucose, lactate, and total protein) in “Small” (total weight: 50 ± 5.1 g, age: 273 days after eclosion (DAE)) and “Big” (total weight: 101.3 ± 10.4 g, age: 571 DAE) white seabream (Diplodus sargus) subjected to an Amyloodinium ocellatum infestation (8000 dinospores mL−1) during a 24-h period. The results demonstrated a strong stress response to A. ocellatum, with marked differences in histopathological alterations, glucose levels, and some hematological indexes between the fish of the two treatments. This work elucidates the need to take in account the size and age of the fish in the development and establishment of adequate mitigating measures and treatment protocols for amyloodiniosis.
On Natural Modulational Bandwidth of Deep-Water Surface Waves
Authors: Babanin Alexander V. , Onorato Miguel , Cavaleri Luigi
Published: April 2019

We suggest that there exists a natural bandwidth of wave trains, including trains of wind-generated waves with a continuous spectrum, determined by their steepness. Based on laboratory experiments with monochromatic waves, we show that, if no side-band perturbations are imposed, the ratio between the wave steepness and bandwidth is restricted to certain limits. These limits are consistent with field observations of narrow-banded wind-wave spectra if a characteristic width of the spectral peak and average steepness are used. The role of the wind in such modulation is also discussed.
Effects of Calcium and Manganese on Sporulation of Bacillus Species Involved in Food Poisoning and Spoilage
Authors: Sinnelä Martti Tapani , Park Young Kyoung , Lee Jae Hoan , Jeong KwangCheol Casey , Kim Young-Wan , Hwang Han-Joon , Mah Jae-Hyung
Published: April 2019

Spores are resistant against many extreme conditions including the disinfection and sterilization methods used in the food industry. Selective prevention of sporulation of Bacillus species is an ongoing challenge for food scientists and fermentation technologists. This study was conducted to evaluate the effects of single and combined supplementation of calcium and manganese on sporulation of common pathogenic and food spoilage Bacillus species: B. cereus, B. licheniformis, B. subtilis and B. coagulans. Sporulation of Bacillus vegetative cells was induced on sporulation media supplemented with diverse concentrations of the minerals. Under the various mineral supplementation conditions, the degree of sporulation was quantified with colonies formed by the Bacillus spores. The results revealed that B. licheniformis and B. cereus displayed the weakest sporulation capabilities on media with minimal supplementation levels of calcium and manganese. The lowest sporulation of B. subtilis and B. coagulans was observed on media supplemented with the highest level of calcium and low levels of manganese. Depending on effect of supplementation on sporulation, the Bacillus species were divided into two distinct groups: B. licheniformis and B. cereus; and B. subtilis and B. coagulans. The information provides valuable insight to selectively reduce sporulation of Bacillus species undesirable in the food industry.
Revisiting the Equipartition Assumption in Star-Forming Galaxies
Authors: Seta Amit , Beck Rainer
Published: April 2019

Energy equipartition between cosmic rays and magnetic fields is often assumed to infer magnetic field properties from the synchrotron observations of star-forming galaxies. However, there is no compelling physical reason to expect the same. We aim to explore the validity of the energy equipartition assumption. After describing popular arguments in favour of the assumption, we first discuss observational results that support it at large scales and how certain observations show significant deviations from equipartition at scales smaller than ≈ 1 kpc , probably related to the propagation length of the cosmic rays. Then, we test the energy equipartition assumption using test-particle and magnetohydrodynamic (MHD) simulations. From the results of the simulations, we find that the energy equipartition assumption is not valid at scales smaller than the driving scale of the ISM turbulence (≈ 100 pc in spiral galaxies), which can be regarded as the lower limit for the scale beyond which equipartition is valid. We suggest that one must be aware of the dynamical scales in the system before assuming energy equipartition to extract magnetic field information from synchrotron observations. Finally, we present ideas for future observations and simulations to investigate in more detail under which conditions the equipartition assumption is valid or not.
Genetic Diversity and Population Structure Analysis of Dalbergia Odorifera Germplasm and Development of a Core Collection Using Microsatellite Markers
Authors: Liu Fu-Mei , Zhang Ning-Nan , Liu Xiao-Jin , Yang Zeng-Jiang , Jia Hong-Yan , Xu Da-Ping
Published: April 2019

Dalbergia odorifera T. Chen (Fabaceae) is a woody tree species indigenous to Hainan Island in China. Due to its high medicinal and commercial value, this tree species has been planted over 3500 ha2 in southern China. There is an urgent need for improvement of the D. odorifera germplasm, however, limited information on germplasm collection, conservation, and assessment of genetic resources is available. Therefore, we have built a database of 251 individuals collected across the whole of southern China, which included 42 wild trees and 210 cultivated trees, with the following objectives. (1) Evaluate genetic diversity and population structure of the database using 19 microsatellite markers and (2) develop a core collection for improvement and breeding programs. Totally, the 19 microsatellite markers harbored 77 alleles across the database with the polymorphic information content (PIC) ranging from 0.03 to 0.66. Medium genetic diversity level was inferred by Nei’s gene diversity (0.38), Shannon’s information index (0.65), and observed (0.33) and expected heterozygosity (0.38). Structure analysis showed that four was the optimum cluster size using the model-based Bayesian procedure, and the 251 D. odorifera individuals were grouped into five populations including four pure ones (RP1-4) and one mixed one (MIX) based on their maximum membership coefficients. Among these populations, the expected heterozygosity varied from 0.30 (RP3) to 0.38 (RP4). Analysis of molecular variance (AMOVA) showed 11% genetic variation existed among populations, and moderate population differentiation was inferred by the matrix of pairwise Fst (genetic differentiation among populations), which was in the range of 0.031 to 0.095. Moreover, a core collection of 31 D. odorifera individuals including six wild and 25 cultivated trees was developed, which was only 12.4% of the database but conserved the whole genetic diversity. The results of this study provided additional insight into the genetic structure of the large D. odorifera germplasm, and the core collection will be useful for the efficient and sustainable utilization of genetic resources, as well as efficient improvement in breeding programs.
Characterization and Transcriptome Studies of Autoinducer Synthase Gene from Multidrug Resistant Acinetobacter baumannii Strain 863
Authors: Ng Chung-Kiat , How Kah-Yan , Tee Kok-Keng , Chan Kok-Gan
Published: April 2019

Quorum sensing (QS) is a cell-to-cell communication system that uses autoinducers as signaling molecules to enable inter-species and intra-species interactions in response to external stimuli according to the population density. QS allows bacteria such as Acinetobacter baumannii to react rapidly in response to environmental changes and hence, increase the chances of survival. A. baumannii is one of the causative agents in hospital-acquired infections and the number of cases has increased remarkably in the past decade. In this study, A. baumannii strain 863, a multidrug-resistant pathogen, was found to exhibit QS activity by producing N-acyl homoserine lactone. We identified the autoinducer synthase gene, which we named abaI, by performing whole genome sequencing analysis of A. baumannii strain 863. Using high resolution tandem triple quadrupole mass spectrometry, we reported that abaI of A. baumannii strain 863 produced 3-hydroxy-dodecanoyl-homoserine lactone. A gene deletion mutant was constructed, which confirmed the functionality of abaI. A growth defect was observed in the QS-deficient mutant strain. Transcriptome profiling was performed to determine the possible genes regulated by QS. Four groups of genes that showed differential expression were discovered, namely those involved in carbon source metabolism, energy production, stress response and the translation process.
Spatial and Temporal Variations in the Incidence of Dust Storms in Saudi Arabia Revealed from In Situ Observations
Authors: Albugami Sarah , Palmer Steven , Cinnamon Jonathan , Meersmans Jeroen
Published: April 2019

Monthly meteorological data from 27 observation stations provided by the Presidency of Meteorology and Environment (PME) of Saudi Arabia were used to analyze the spatial and temporal distribution of atmospheric dust in Saudi Arabia between 2000 and 2016. These data were used to analyze the effects of environmental forcing on the occurrence of dust storms across Saudi Arabia by considering the relationships between dust storm frequency and temperature, precipitation, and wind variables. We reveal a clear seasonality in the reported incidence of dust storms, with the highest frequency of events during the spring. Our results show significant positive relationships (p < 0.005) between dust storm occurrence and wind speed, wind direction, and precipitation. However, we did not detect a significant relationship with temperature. Our results reveal important spatial patterns, as well as seasonal and inter-annual variations, in the occurrence of dust storms in Saudi Arabia. For instance, the eastern part of the study area experienced an increase in dust storm events over time, especially in the region near Al-Ahsa. Similarly, an increasing trend in dust storms was also observed in the west of the study area near Jeddah. However, the occurrence of dust storm events is decreasing over time in the north, in areas such as Hail and Qaisumah. Overall, the eastern part of Saudi Arabia experiences the highest number of dust storms per year (i.e., 10 to 60 events), followed by the northern region, with the south and the west having fewer dust storm events (i.e., five to 15 events per year). In addition, our results showed that the wind speeds during a dust storm are 15–20 m/s and above, while, on a non-dust day, the wind speeds are approximately 10–15 m/s or lower. Findings of this study provide insight into the relationship between environmental conditions and dust storm occurrence across Saudi Arabia, and a basis for future research into the drivers behind these observed spatio-temporal trends.
GIS-Based Rockfall Susceptibility Zoning in Greece
Authors: Saroglou Charalampos
Published: April 2019

The assessment of rockfall risks on human activities and infrastructure is of great importance. Rock falls pose a significant risk to (a) transportation infrastructure, (b) inhabited areas, and (c) Cultural Heritage sites. The paper presents a method to assess rockfall susceptibility at national scale in Greece, using a simple rating approach and Geographic Information Systems (GIS) techniques. An extensive inventory of rockfalls for the entire country was compiled for the period between 1935 and 2019. The rockfall events that were recorded are those which have mainly occurred as distinct rockfall episodes in natural slopes and have impacted human activities, such as roads, inhabited areas, and archaeological sites. Through a detailed analysis of the recorded data, it was possible to define the factors which determine the occurrence of rockfalls. Based on this analysis, the susceptibility zoning against rockfalls at the national scale was prepared, using a simple rating approach and GIS techniques. The rockfall susceptibility zoning takes into account the following parameters: (a) the slope gradient, (b) the lithology, (c) the annual rainfall intensity, (d) the earthquake intensity, and (e) the active fault presence. Emphasis was given on the study of the earthquake effect as a triggering mechanism of rockfalls. Finally, the temporal and spatial frequency of the recorded events and the impact of rockfalls on infrastructure assets and human activities in Greece were evaluated.
Evidence of Differences and Discrimination in the Delivery of Care: Colorectal Screening in Healthy People and in the Care and Surveillance of Patients with Inflammatory Bowel Disease
Authors: Farrukh Afffa , Mayberry John
Published: April 2019

Objectives: In this review the management of colorectal disease will be investigated as an exemplar of common practice in the UK in an attempt to identify factors responsible for the more general experiences of patients from ethnic minorities. Within this field such populations have a lower uptake of cancer screening programmes and their experience of day-to-day care for chronic gastrointestinal disorders is poor. Study design: PubMed and Google Scholar were reviewed in 2016 to identify publications concerning colorectal screening in patients with inflammatory bowel disease and healthy communities. Methods: Data were extracted from each paper and the references exploded to identify other potential reports. Results: It is reported that barriers exist both at individual and access levels but little has been done to overcome these. There have been a number of suggestions as to how to provide equitable access, but there is a clear need to ensure that these are evidence based and have been tested and shown to be effective in clinical trials. Conclusions: Clearly, current systems of surveillance and screening will only make a difference if they provide effective and acceptable services to all potential clients. Most programmes fail to address the specific risks and anxieties of minority groups, which are thought to be poorly compliant. This review considers those factors that may play a part and suggests approaches that could overcome these deficiencies. Some clues as to these factors may come from work with patients with chronic disorders.
Personal Narratives of Illness: Redressing Madness in the Singaporean Fiction of Amanda Lee Koe
Authors: Ho Hannah Ming-Yit
Published: April 2019

Amanda Lee Koe’s short stories (2013) redress the limited tolerance for the mad citizen-subject, whose subjectivity is obscured, if not erased, by medical prescriptions. Official and often state-sanctioned conceptualizations of the peculiar mind are grievously justified in behavioral manifestations deemed socially unacceptable. Koe’s stories about idiosyncratic Singaporeans illustrate the way personal experiences—of memory loss, homosexual tendencies, and emotional self-expressions—are informed by, and in turn inform, the biopolitical regulation of Singaporean citizens rendered objects of biopower. In this way, her stories invite a meditation on the state, people and power. Foregrounding fractured and unorthodox characters, these stories serve to intensify individual voices articulated in personal narratives addressing affective experiences, including sadness culminating in loneliness. Furthermore, the stories attest to socially constructed norms instigating the repudiation and criminalization of sexual deviants. Significantly, they add to the “cultural apparatus”—which C.W. Mills defines as “the source of Human Variety—of styles of living and of ways to die”—by questioning the nation’s ideological imperatives, including heterosexual norms, social insistence on mono-cultural marriages and state/family-endorsed medical intervention. Offering a critique of ideological state apparatus embedded within the power structures inherent to psychopathology, Koe’s Ministry of Moral Panic challenges the established ways of viewing “Others” who are ostensibly “mad”. Consequently, her stories mediate a broadening human experience, by calling for inclusivity amid the social rejection and insular treatment of afflicted subjects with alleged disorders.
The Prague Orgy: The Life of Writers in a Totalitarian State According to Philip Roth
Authors: Sýkora Michal
Published: April 2019

This paper deals with the way Philip Roth depicted writers in Czechoslovakia in the 1970s in his novella The Prague Orgy, the final part of the Zuckerman Bound tetralogy. Researchers often read The Prague Orgy in the context of the entire tetralogy and accentuate the contact with Jewish topics. The primary focus of the paper is how Roth views Czech writers and their lives through the eyes of his long-term hero (and fictional alter-ego) Nathan Zuckerman and how he perceives life in a totalitarian state. The Prague Orgy is discussed as a somewhat abstract story about the writer’s freedom and responsibility of their work. There are three types of writers in The Prague Orgy: The émigré (Sisovsky), the dissenter (Bolotka), and the pro-regime (Novak). Each of them, in an interview with Roth’s hero, formulates his attitude to the regime. Zuckerman is fascinated by the life of opposition artists, their experience of freedom (realized in the private sphere), and the social response to their work. Although the reality of life in Czechoslovakia under communism is not the main topic of the novella, the paper concludes that the depiction of life of Czech underground intellectuals interested mostly in sex is in consonance with the picture of Czech dissent in official regime propaganda.
A Supervised Pattern Analysis of the Length of Stay for Hip Replacement Admissions
Authors: Zikos Dimitrios , Shrestha Ashara , Colotti Taylor , Fegaras Leonidas
Published: April 2019

Hip replacement is the most common surgical procedure among Medicare patients in the US and worldwide. The hospital length of stay (LOS) for hip replacement admissions is therefore important to be controlled, contributing to savings for hospitals. This study combined medical claims and hospital structure and service data to examine LOS fluctuations and trends, and admission distribution patterns, during weekdays, for hip replacement cases. The study furthermore examined associations of these patterns with the LOS performance. Most hospitals were found to admit hip replacement cases at the start of the week (Monday through Wednesday). There is an upward LOS trend as we approach late weekday admissions. Multiple linear regression analysis showed that LOS weekday inconsistencies, a large proportion of hip replacement admissions on Thursday and Friday, the government ownership status, the bed size, and the critical access status are associated with an increased LOS. On the other hand, the rate of hip replacement admissions over total ones, and the hospital being accredited, are associated with a lower LOS. Findings stress out the need for hospitals to maintain an effective and balanced distribution of hip replacement admissions, evenly during the week, and the need for standardized case management, to avoid practice variability and, therefore, LOS fluctuations for their hip replacement cases.