bluuebunny/crossref_metadata_2025 huggingface dataset:
The Law of Supersystem Overreaction and the Extreme Principle
Authors:   Independent researcher, Gribkov Andrey A.
Published: October 2023

The article’s subject of study is the extreme principle, according to which the system behaves in such a way that some value characterizing its activity takes an extreme (minimum or maximum) possible value. The content of the extreme principle, its formulations, as well as a special case of its manifestation in the form of the principle of least action are considered. The connection between the extreme principle, on the one hand, and primary properties and basic laws of being, on the other hand, is revealed. The sought connection can be built through the law of supersystems overreaction formulated by the author in the previous paper, which is one of the prima-ry laws of being and is determined from the analysis of evolution at the lowest level of material structures. The dissipative nature of all existing real systems means their existence at the expense of supersystems’ resources and determines the limitations of behavior, which in the framework of epistemology can be formalized in the form of an extreme principle.
Training of Party-Soviet Cadres in the USSR: A Review of Soviet Historical-Party and Modern Russian Historiography
Authors:   North-West Institute of Management – Branch of the Russian Academy of National Economy  and Public Administration under the President of the Russian Federation, Shcherbina Elena Yu.
Published: October 2023

The article analyzes the Soviet historical-party and modern Russian historiography devoted to the problems of training nomenklatura cadres in the USSR. Eight chronological stages of the development of national historical knowledge about the preparation of party reserves are distinguished: 1) 1918–1927; 2) 1928–1955; 3) 1956–1964; 4) 1965–1985; 5) 1985–1991; 6) 1992–1999; 7) 2000–2013; 8) 2014 – present. It is emphasized that Russian historiography developed from the theoretical generalization of practical issues of the construction of school for training Soviet government and Communist Party workers and communist higher educational institu-tions in the country to large regional investigations of the nomenclature corps of the Soviet period and factual reviews of the history of the development of certain higher party educational institutions of the CPSU. The change in the social image of managers that took place by the end of the Soviet period and the replacement of the mobilization approach to the recruitment of nomenklatura personnel by a competence-based approach led to the curtailment of the CPSU party educational institutions.
Mapping Method as a Tool for Creating Museum Clusters (On the Example of the Crimea)
Authors:   Crimean University of Culture, Arts and Tourism, Simferopol, Mikitinets Olga I. , Shilina Victoria А. ,   Crimean University of Culture, Arts and Tourism, Simferopol
Published: October 2023

The article reveals the specifics of applying the method of mapping the museum sphere, in particular, in the process of creating museum clusters as one of the productive tools in the perception and functioning of the museum as a significant part of the regional economy. This need is due to the trends of the modern world, where museums manifest themselves as an important economic industry, significantly influencing the cultural and economic picture of the region. At the same time they continue to play an important role in the preservation of cultural heritage, providing access to history and art, in the issues of ethnic self-identity, etc. Developing methods and technologies for the creation of museum clusters determine the relevance of this article, in partic-ular, the application of the mapping method. The research methodology is presented both by general scientific (analysis and synthesis) and special (design, mapping) methods. Research interest is focused on the theoreti-cal and applied features of the application of museum network mapping. As a result of the study, the authors identified regional features of the Crimean museum network in the context of clustering. In particular, O.I. Mikitinets, as a result of a deep and comprehensive analysis of works in the field of mapping, and, in particular, cultural mapping, agrees with the statement of A.I. Zyryanov is that this method only visualizes reality, and does not change it, which eliminates its shortcomings, provided that the data shown on the map fully corresponds to reality. The conducted theoretical analysis made it possible for V.A. Shilina to out-line the directions of applied research in the field of mapping the museum environment of the Crimean region. In this context, both popular (South Coast) and developing (Northern Crimea) museum areas of the peninsula were reflected visually. This in turn characterizes the mapping method as one of the innovations, the introduc-tion of which will help to expand the museum experience.
Problems of Legal Culture Formation in Modern Socio-Cultural Space
Authors:   Independent researcher, Kisenko Nikolay P.
Published: October 2023

The article postulates that progress in the development of the rule of law is inconceivable without the formation of legal culture. Such a process is complex due to the multidimensionality of legal culture, included in the gen-eral culture of the people, and the secondary nature of legal values in relation to general cultural values. The author distinguishes objective and subjective factors of legal culture formation in the modern socio-cultural space. Objective factors include globalization processes in the world that have fully affected Russian society and the state, as a result of which there is an undermining of the foundations of Russian legal culture and de-formation of the targeted mechanisms for its formation, such as legal education and upbringing, which inevita-bly leads to destructive processes in other subsystems – economic, political and spiritual. Among the subjective factors, the author highlights the value orientations and attitudes of all subjects-bearers of legal consciousness towards legal behavior and involvement in positive changes in legal reality, which act as internal motivators of external forms of expression of the level of legal culture. The author emphasizes the importance of reforming the institute of education and upbringing as the main mechanism for the formation of subjective factors of legal culture in the context of legalization of all spheres of life of modern man, and actualizes the need to create a unified system of competencies in the field of legal knowledge, skills and abilities for graduates of schools, universities and colleges of non-legal areas in accordance with the strategy of building a law-governed state. The importance of the formation of value orientations and attitudes for carriers of professional legal conscious-ness is stressed, as the level of legal culture depends on personal example and quality of performance of offi-cial duties. Among the measures directly dependent on state institutions is the formation of a strong state ideol-ogy based on the national foundations of Russian culture, taking into account the factors of multi-ethnicity and multi-confessionalism, which act as a platform for the legal culture of the individual, group and society as a whole.
Revolutionary and Evolutionary Ways of Development: Historical and Philosophical Analysis
Authors:   State Agrarian University of the Northern Trans-Urals, Berezuev Evgeny A.
Published: October 2023

In modern society, unfortunately, the danger of destructive revolutionary upheavals is still not understood seri-ously enough. The events in Ukraine in 2014 serve as a reminder to all subsequent generations of how a storm of mass emotions, not supported by an understanding of the expediency of the social order, can lead into a long-term mass catastrophe, in general, a fairly successful economic and social society. This is precisely why a balanced comparative analysis of revolutionary and evolutionary ways of society's development is necessary to explain and understand the only way of normal development – evolutionary progressive development, oppo-site to destructive revolutionary development. Experience in history has shown that mass terror and unjustified social experiments in the field of economy and social life have devastating effects on the state. A case in point is Western Europe during the crisis of feudal relations and Russia in the early XX century.
Branding of the Republic of Adygea Based on Traditional and Innovative Technologies
Authors:   Adyghe State University, Zhade Zuriet A.
Published: October 2023

Based on the analysis of the main brands of the Republic of Adygea, the article provides their classification taking into account the historical, natural and ethno-cultural characteristics of the region. Branding is considered as one of the directions of identity politics, along with symbolic politics and memory politics. The review of modern publications and regulatory documents devoted to branding policy in the republic is carried out. The main traditional and innovative practices and branding trends of the region are outlined. It is argued that in 2022 the traditional technologies were supplemented by innovative ones, timed to the centenary of Adygea’s statehood, which contributed to the full promotion of the region’s brands. Conclusion dwells upon the fact that regional institutions of power implement a successful identity policy, the component part of which is the brand-ing of the region. This prospect is complemented by the recommendations for further improvement and promo-tion of Adygea’s brands.
Intellectual Law and Scientific and Educational Interaction of the EAEU Member States
Authors:   Financial University under the Government of the Russian Federation, Basova Anastasiya G. , Ogloblina Elizaveta V. ,   Financial University under the Government of the Russian Federation
Published: October 2023

The article analyzes two main sectors that are the drivers of socio-economic development of the EAEU mem-ber states: the scientific and educational sector and the intellectual property (hereinafter – IP) sector. Using var-ious theoretical and methodological approaches of scientific research, the main barriers to development and risks of strengthening the interaction of states in these areas are identified. The authors, based on the study of international practice, as well as national differences of the member states, highlight measures to strengthen cooperation, in particular, recommendations for the state bodies of the Union and private business. Systemati-zation and harmonization of these areas of cooperation of the Union will increase the level of integration inter-action in the conditions of digital development of the global economy, as well as to ensure effective protection of IP rights, to form a highly qualified pool of Eurasian youth.
Diseño y evaluación bioclimática para Museo del Agua en el municipio de Tecámac, Estado de México
Authors:   , Ramírez Sánchez Iván
Published: October 2023

El municipio de Tecámac en el Estado de México actualmente cuenta con 2 plantas de tratamiento de aguas residuales que son operadas por el sistema ODAPAS, una ubicada dentro del complejo deportivo “Estado de México” que le provee de agua para riego y mantenimiento de áreas verdes dentro de la misma y la otra se localiza dentro del parque ecológico y deportivo “Sierra Hermosa” que suministra agua tratada para la laguna ubicada dentro del parque. En colaboración de alumnos del posgrado en diseño bioclimático de la Universidad Autónoma metropolitana unidad Azcapotzalco con el municipio de Tecámac, se desarrollará el diseño del “Museo del Agua” como proyecto de aplicación de los conocimientos adquiridos por parte del estudiante durante la especialización en diseño ambiental, área de arquitectura bioclimática. Las autoridades municipales de Tecámac hacen mención que son uno de los pocos municipios que cuentan con 2 plantas tratadoras de agua residuales rehabilitadas y funcionales, por lo tanto, requieren de un espacio arquitectónico donde se realice las siguientes actividades: difusión de la cultura del agua y funcionamiento de las plantas tratadoras. El espacio contara con un diseño y características bioclimáticas adaptadas al clima del sitio. Contará con 5 salas de exhibición permanente con las temáticas siguientes: sala 1 Ciclo del agua, sala 2: Agua potable, sala 3: Cultura del agua, sala 4 Aguas residuales, sala 5: Tratamiento del agua. Además de un auditorio con capacidad de más233 usuarios además de un edificio administrativo que albergara un laboratorio para análisis de muestras, sala de juntas, cocina y comedor para empleados.
Ludus Regularis: The Clergy Game
Authors: Cardoso Carla
Published: October 2023

Ludus Regularis is a board game with dice, from the 10th century, which came to our knowledge through literary sources. The rules of the game were kept by the historian Balderic, in the 11th century, in a book edited in 1615 by Colvener: Chronicon Cameracense et Atrebatense, and reedited in 1834 by André Le Glay, in Paris: Chronique d’Arras et de Cambrai.

By studying the probabilities involved in the dynamics of the game Ludus Regularis, it is possible to advance that the author’s concerns were mainly centred on presenting a game that followed Catholic doctrine.

Ludus Regularis is a carefully conceived game, using dice commonly inaccessible to members of the clergy, in a context of deep Christian religious symbolism.
Maldivian Chess (Raazuvaa)
Authors: Markov Georgi
Published: October 2023

Traditional Maldivian chess rules are presented and their similarity to pre-19th century Turkish chess is discussed, with musings on the potential role of Ottomans in spreading the “new game” invented in Europe – but played in their own manner – throughout the Muslim world and beyond.
Reassessing Roman and Late Antique ‘Marbles Lanes’: One Game or Many?
Authors: Penn Tim , Courts Summer , Schädler Ulrich
Published: October 2023

Characterised by the presence of multiple depressions or pockets in a variety of arrangements, and, in some cases, the presence of a single, double, or triple ‘start line’ carved into horizontal stone surfaces, marble lanes in their variety of forms open a window onto ancient play that few have looked through. Thought to be a playing surface for some kind of throwing or rolling game which involved the use of glass or ceramic spheres, Roman marble lanes have received comparatively little attention in the recent upswing of scholarship on ancient play, partially as a result of the relative dearth of textual and iconographic sources discussing or depicting their usage, but these playing surfaces nevertheless represent a major corpus of ludic material. This contribution summarises past work on marble lanes before exploring the limited textual and iconographic source material related to playing with marbles. It offers a tentative new typology by which to categorise marble lanes and a non-exhaustive list of these playing surfaces recorded at archaeological sites around the Mediterranean. It then moves onto a discussion of the game/games that may be played on these boards, arguing that the wide variations in the different layouts for marble lanes may indicate that they were used not for one tightly-defined game, but more likely facilitated the playing of a loosely connected family of games, with implicaitons for how we think about communities of play in the past.
Economically optimised target state of uneven-aged forest management for main forest types in Slovakia
Authors: Roessiger Joerg , Kulla Ladislav , Murgaš Vlastimil , Sedliak Maroš , Šebeň Vladimír
Published: October 2023

The study presented a bioeconomic modelling approach for an uneven-aged mixed forest management planning. Regression models for transition (increment), and ingrowth (regeneration) used the National Forest Inventory (NFI) of Slovakia and regional inventory data. Mortality was based on salvage logging records. Models were specific for five tree species within three forest types (FT) (oak with hornbeam and beech, beech, mixed fir-beech-spruce). Net timber prices were calculated with regard to stem quality. Tree growth depended on crown characteristics. The regression models were adjusted to three main geobiotope (GBT) sites per FT. Forest growth was simulated with the density-dependent stand-level matrix transition model. Financial optimisation of harvest was sensitive to an interest rate. Long-time optimisation stabilised in a steady state equilibrium characterised by a stable diameter distribution. Target diameters were specific for site and tree species, and were highest for fir, a dense crown, a good stem quality, and a lower interest rate. Standing timber volume varied from 150 m3 ha–1 (oak forests, 2% interest rate) to 400 m3 ha–1 (beech and fir-beech-spruce forests, 0.5% interest rate). Harvested volume varied from 38 to 93 m3 ha–1 per 10 years, stand basal area (ba) varied from 19 to 36 m2 ha–1 depending on the site, timber price, and interest rate. The discussion pointed out that the relative low volume of the oak FT resulted from the light-demanding characteristics of oak. The mean of oak mosaic structures was lower compared to the high level of more storeys present in the single tree selection structures in beech and mixed fir-beech-spruce forests.
Factors Influencing Scabies Infection at the Al-Amin Islamic Boarding School in Sukabumi
Authors: Adinata Thariq Mahathir , Sulistiyaningrum Sri Katon , Saenong Rizqa Haerani , Marindawati Mieke
Published: October 2023

Background: Scabies are a common health problem in many countries, mainly in densely populated areas. The prevalence of scabies in first community health services in Indonesia is 5.6-12.9%. Scabies infection is associated with a lack of health education, small bedrooms with too many people living in poor sanitation, and a crowded environment found in public Islamic boarding schools in Indonesia. Purposes: To investigate the correlation between characteristics, knowledge, and hygiene behavior with the incidence of scabies among boarding school students. Methods: This is a cross-sectional analytic study; 150 subjects were conducted in this study, and eight subjects were excluded. Subjects were conducted for an examination and filled out a questionnaire to diagnose the scabies infection. Bivariate and multivariate logistic regression analyses were used to assess the risk factors of subjects associated with scabies infection. Results: 142 subjects were analyzed, and 77 (54.2%) were diagnosed with scabies. From bivariate analyses, we found that gender (p<0.0001), level of education (p<0.0001), and clean behavior (p<0.0001) were associated with scabies outcome. Male (p=0.015, OR 95% CI 4.3 (1.3 – 14.3), higher level of education (p=0.012, OR 95% CI 8 (1.58 – 41.2), and poor hygiene behavior (p<0.0001, OR 95% CI 1 (1.02 – 1.09) were independently associated with scabies infection. Conclusions: There was a significant association between gender, level of education, and clean behavior with scabies outcome. Males, low levels of education, and poor hygiene behavior increase the incidence of scabies infection. Age, nutritional status, and knowledge of scabies were not associated with scabies outcome.
Gonzalo M. Pavés (2021). Bigas Luna. El gran fabulador. Sueños, obsesiones y algunas otras ibéricas perversiones. Laertes.
Authors:   Universidad de La Laguna (España), Ramírez Guedes Enrique
Published: October 2023

Reseña
Abismos, espejos y ojos: el terror elevado a través de Jordan Peele
Authors:   Universidad de La Laguna, Delgado Rodríguez Darío
Published: October 2023

High terror is characterized by its minimalist stylization and profound themes that explore dark aspects of the human condition, diverging from the conventional resources of commercial horror cinema. Using the cinema of Jordan Peele as an example, this article seeks to delve into this cycle of films, deepening into their techniques and the meanings that are hidden beneath the terror narratives they unfold. Likewise, we propose that as powerful generators of meanings, this is something we find present in horror cinema throughout the history of the medium.
PHARMACOKINETIC STUDY OF LACTOBACILLI AND BIFIDOBACTERIA STRAINS IN MICE
Authors:   Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Zoruk P.Yu. , Boldireva D.Y. , Olekhnovich E.I. , Klimina K.M.
Published: October 2023

Immunological approaches have demonstrated high efficiency in the fight against various types of malignant tumors. However, some patients do not form the expected positive response to immunotherapy. Among other factors, this effect is also associated with the composition of intestinal microbiota [1–3]. Based on previously analyzed data, taxonomic groups of bacteria associated with a positive response to cancer immunotherapy were identified, in particular bacteria of genus Bifidobacterium adolescentis. In a pilot experiment on laboratory mice (females, С57Bl/6) we studied the pharmacokinetics of excretion of lactobacilli and bifidobacteria strains in mice and biological diversity in fecal samples.
THE EVOLUTION OF THE AZGP1 TUMOR GENE AND THE FORMATION OF ITS FUNCTIONS ASSOCIATED WITH OBESITY
Authors:   Saint Petersburg State University, Kadirova F.R. , Matyunina E.A. , Kurbatova T.V. , Kozlov A.P. ,   Research Institute of Hygiene, Occupational Pathology and Human Ecology, Saint Petersburg,   Vavilov Institute of General Genetics RAS, Moscow,   The Biomedical Center, Saint Petersburg
Published: October 2023

The numerous similarities found between adipose tissue and tumors suggest the possible evolutionary origin of mammalian adipose tissue from some hereditary benign mesenchymal tumors [1]. Genes that originated in fish and were expressed in their tumors have been described [3], and their human orthologues (AZGP1 and others) [2] acquired functions associated with a fatty organ.
MULTILEVEL MATHEMATICAL MODEL OF EPILEPTIC SEIZURES
Authors:   Sirius University of Science and Technology, Sochi, Kondrakhin P.Yu. , Kolpakov F.A. ,   Federal Research Center for Information and Computational Technologies, Novosibirsk
Published: October 2023

The paper presents novel developed modular mathematical model of epileptic seizures, obtained by combining and modifying existing models of epilepsy, which for the first time makes it possible to simulate the dynamics of seizure onset, propagation and termination simultaneously at the cellular and regional levels of brain organization.
STUDY OF THE MECHANISM OF PEROXIDASE ACTIVITY OF CYTOCHROME C IN COMPLEX WITH CARDIOLIPIN
Authors:   Pirogov Russian National Research Medical University, Moscow, Levchenko I.N. , Vladimirov G.K. , Volodyaev I.V. ,   Sechenov First Moscow State Medical University,   Lomonosov Moscow State University
Published: October 2023

It is shown that: 1) the points of peroxidase activity and quantum yields are significantly higher in the presence of the natural dye coumarin C-334 than in the case of its own non-activated glow or in the case of the natural dye coumarin C-314; 2) peroxidase activity depends not only on the concentration of cytochrome C in the composition of the cytochrome C catalyst in combination with cardiolipin, but also on the relationship between the native form of cytochrome C and partially denatured.
STUDY OF THE IMMUNE TRANSCRIPTOME OF HUMAN ERYTHROID CELLS BY SCRNA-SEQ
Authors:   Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Perik-Zavodskii R.Yu. , Perik-Zavodskaia O.Yu. , Shevchenko Yu.A. , Volynets M.O. , Alrhmoun S. , Denisova V.V. , Zaitsev K.V. , Sennikov S.V. ,   Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology, Novosibirsk,   Novosibirsk State University,   Siberian Federal Research and Clinical Center FMBA, Tomsk
Published: October 2023

Erythroid cells (ECs) are precursors of erythrocytes that have been shown to have immunoregulatory properties. For a detailed study of the regulatory mechanisms of ECs, we analyzed the immune transcriptome of ECs in the bone marrow, fetal liver, and cord blood using single cell RNA sequencing. We found differentially expressed genes ARG1, LGALS3, CXCL5, and VEGFA at different stages of differentiation and ontogeny.
MITOCHONDRIAL DNA AH > GH MUTATION AND ITS ASSOCIATIONS WITH BIRDS LIFE-HISTORY TRAITS
Authors:   Center for Mitochondrial Functional Genomics, Immanuel Kant Baltic Federal University, Kaliningrad, Gusarov Y.S. , Mikhailova A.G. , Iliushchenko D.V. , Efimenko B.E. , Gunbin K.V. , Burskaya V.O. , Popadin K.Y. ,   University of Antwerp, Belgium,   Ecole Polytechnique Federale de Lausanne, Switzerland
Published: October 2023

Mitochondria is a double-membrane organelle, which is always under pressure of reactive oxygen species. Those molecules are the main reason for Ah > Gh (h — heavy strand) mutation both in fishes [1] and mammals [2]. H
REACTION-DIFFUSION MODEL OF MYOCARDIAL INFARCTION
Authors:   Federal Research Center for Information and Computational Technologies, Novosibirsk, Tsgoev Ch.A. ,   Novosibirsk State University
Published: October 2023

A series of numerical studies of the necrotic cell death process and inflammatory response in ischemic myocardial infarction has been performed using a minimal mathematical model of aseptic inflammation dynamics. The main attention is focused on the analysis of regularities of spatial and temporal development of the process. Evaluations of the efficacy of a number of hypothetical therapeutic strategies have been performed. The modeling results are in agreement with experimental data.
DIFFERENTIAL ANALYSIS OF GENE EXPRESSION IN BRAIN TISSUES OF MICE WITH MPTP-INDUCED PARKINSONISM USING THE DESEQ2 SOFTWARE PACKAGE
Authors:   National Research Center “Kurchatov Institute”, Moscow, Chumakova A.B. , Rudenok M.M. , Semenova E.K. , Partevyan S.A. , Rybolovlev I.N. , Shadrina M.I. , Vlasov I.N.
Published: October 2023

This work is devoted to the development of a software pipeline for differential analysis of gene expression using the DESeq2 software package in the R programming language. The research was conducted in order to identify differentially expressed genes during the development of neurodegenerative processes in the brain tissues of mice with MPTP-induced Parkinson’s disease.
ENGINEERING POLYMICROBIAL AGGREGATES FOR PETROLEUM BIOTECHNOLOGY
Authors:   Aramco Innovations, LLC, Moscow, Rybkin I. , Humam A. , Alahmari M. , Orlov M. ,   Saudi Aramco, Dhahran, Saudi Arabia
Published: October 2023

Bacterial surface modification through electrostatic deposition of polyelectrolytes with opposite charges enables the assembly of various microbial cells into cellular aggregates that have versatile applications. This study showcases cutting-edge methods for altering the surface charge and illustrates numerous scenarios such as wastewater treatment or physiology control of cells, which can be utilized in petroleum biotechnology applications.
ANTISENSE OLIGONUCLEOTIDES RELEASING CASSETTE FOR CANCER THERAPY
Authors:   SCAMT Institute, ITMO University, Saint Petersburg, Salimova A.A. ,   Almetyevsk State Oil Institute,   Gymnasium No. 526, Saint Petersburg,   Chemistry Department, University of Central Florida, Orlando, USA,   Burnett School of Biomedical Sciences, University of Central Florida, Orlando, USA,   National Center for Forensic Science, University of Central Florida, Orlando, USA, Drozd V.D. , Rybalko D.S. , Eldeeb A.A. , Dedovskayav A.A. , Kolpashchikov D.M.
Published: October 2023

Cancer gene therapy is a challenging area of research. Cancer therapy aims to target mutated genes that play crucial roles in tumorigenesis. However, this approach often encounters challenges such as low efficiency and off-target effects. Consequently, the number of drugs approved for clinical use remains limited. Here, we suggest a novel approach in cancer therapy — a DNA construct that is able to target vital housekeeping genes in a cancer-marker dependent manner.
EXPLORING THE USE OF HERBAL EXTRACTS FOR ACNE TREATMENT
Authors:   Moscow Institute of Physics and Technology, Dolgoprudny, Sapozhnikova E.N.
Published: October 2023

According to WHO, about 85 % of the world’s adolescents suffer from acne. However, due to the increase in the number of new infections and the increase in the number of cases of bacterial resistance to antibiotics, it is necessary to study the antibacterial properties of plants growing in our habitat strip. These drugs, while inhibiting the growth of pathogens and reducing the synthesis of sebaceous glands, will expand the list of medicines to combat acne.
ENRICHMENT OF YEAST WITH MICROELEMENTS AS AN OPPORTUNITY TO ELIMINATE MICRONUTRIENT DEFICIENCY IN NUTRITION
Authors:   All-Russian Research Institute of Food Biotechnology, Moscow, Yuraskina T.V. , Sokolova E.N. , Fursova N.A. , Serba E.M.
Published: October 2023

Studies in the field of micronutrient enrichment of yeast with 4 different trace elements: iron (Fe), copper (Cu), zinc (Zn), chromium (Cr) were carried out. The possibility of yeast enrichment with the production of bio-preparations with both a monomicroelement profile and combined fortification with two microelements was experimentally confirmed. The possibility of enriched yeast usage in food production was confirmed. The possibility of enrichment of inactivated yeast fermentolysates was studied. The perspective of baker’s yeast as a source of bio-preparations of trace elements was proved.
BIONFORMATIC ANALYSIS OF THE GENOMES OF WILD AND DOMESTIC ANIMALS OF THE SPECIES SUS SCROFA TO UPGRADE THE “SWINE/WILD BOAR” DIFFERENTIATION MODEL
Authors:   Institute of Genetics and Cytology NAS, Minsk, Belarus, Kipen V.N. , Snytkov E.V.
Published: October 2023

Using bioinformatic methods, the analysis of projects on genome-wide sequencing (NGS) of animals of the species Sus scrofa scrofa (wild boar) and Sus scrofa domesticus (domestic pig) was carried out, as a result of which polymorphisms (SNPs) with significant differentiating potential were determined.
CO-EXPRESSION OF VITREOSCILLA HAEMOGLOBIN IN E. COLI TO INCREASE STRAIN RESISTANCE TO LOW OXYGEN CONTENT DURING HIGH-DENSITY CULTIVATION
Authors:   Pharm-Holding, CJSC (Geropharm R&D Center), Saint Petersburg, Bochkareva M.D. , Grechko D.A. , Kazanova N.K. , Khasanshina Z.R. , Latypov V.F.
Published: October 2023

One way to increase the productivity of producer strains is to increase their tolerance to oxygen deficiency, which occurs during high-density cultivation in industrial bioreactors. In this work, the tolerance of cells to oxygen deficiency was increased by Vhb co-expression, which made it possible to increase oxidative activity of cells and specific productivity of the strain during cultivation in bioreactors.
COMPARATIVE GENETIC AND PHENOTYPIC ANALYSIS OF NEAR-ISOGENIC BARLEY LINES WITH ANTHOCYANIN GRAIN PIGMENTATION
Authors:   Institute of Cytology and Genetics SB RAS, Novosibirsk, Molobekova C.A. ,   Novosibirsk State University, Kukoeva T.V. , Shoeva O.Y.
Published: October 2023

Here, near-isogenic barley lines (NILs) were used to study associations between genomic regions, responsible for anthocyanin synthesis in grain, and yield-related traits. Biochemical and yield component analyses showed that NILs differ from parental varieties in key agronomic traits. Genotyping by sequencing was performed for mapping the regions responsible for the observed phenotypic changes.
OBTAINING FULL SEQUENCES OF CD8+ T-CELL RECEPTORS SPECIFIC TO THE TUMOR-ASSOCIATED ANTIGEN HER-2/NEU USING SINGLE CELL MRNA SEQUENCING AND ERGO-II NEURAL NETWORK
Authors:   Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Alrhmoun S. ,   Novosibirsk State University, Perik-Zavodskaia O.Yu. , Volynets M.O. , Lopatnikova Yu.A. , Shevchenko Yu.A. , Fisher M.S. , Perik‑Zavodskii R.Yu. , Kurilin V.V. , Silkov A.N. , Sennikov S.V.
Published: October 2023

The membrane protein HER-2/neu (Human Epidermal Growth Factor Receptor-2) is a tumor-associated antigen, the increased expression of which is observed in various types of tumors, and is associated, in particular, with the most aggressive types of breast cancer and poor clinical prognosis, which makes HER2/neu a universal target for the development of various immunotherapy approaches [1].
Phytochemical Screening, GC-MS Analysis and Total Phenolic Content Determination of Bioactive Compound of Propolis
Authors: . Meenu , Virmani Tarun , Singh Lubhan , Kaushik Rahul
Published: October 2023

India is a hub for a large variety of natural products that have proven their therapeutic efficacy in various ailments and disorders. Despite it, a lot of natural products are yet to be explored, and Indian propolis is one of them. Indian propolis is a waxy natural product obtained from beehives. In this research, phytochemical constituents present in Indian propolis were qualitatively analyzed using phytochemical screening, gas chromatography-mass spectroscopy (GC-MS), and quantitatively using total phenolic content. Phytochemical screening results revealed the presence of total phenolic content, saponins, glycosides, alkaloids, terpenoids, proteins, steroids, flavonoids, oils & resins supported by GC-MS results. The GC-MS analysis revealed the presence of 17 different constituents having higher peak areas. Amongst these 17 bioactive compounds, oxirane, phenyl ethyl alcohol, caffeine, chrysene, riboflavin and 2-aminophenol were found to have higher peak areas of 5.78, 4.58, 4.46, 3.11, 2.65 and 2.56%, respectively. The results of total phenolic contents revealed the presence of 23 different constituents in different concentrations amongst ellagic acid, kaempferol, epicatechin, galangin, and quercetin were present in rich amounts 538.73, 402.34, 234.85, 121.95 and 95.3 μg/kg, respectively. These constituents are well known for their therapeutic efficacy, which is an indication for the exploration of the therapeutic efficacy of Indian propolis in the future.
Synthesis and Characterization of Colloidally Stable Self-crosslinking Water-based Anticorrosive Polymer at Room Temperature
Authors: Omran Khalida A
Published: October 2023

The emulsion polymerization of butyl acrylate (BuA), styrene (St), and 3-isopropenyl-α,α-dimethylbenzyl isocyanate (IPMBI) is presented in this work. Obtain colloidally stable latexes for the poly (BuA/St/IPMBI) system during polymerization and storage for use in a water-based coating formulation. A series of semicontinuous polymerizations were also performed to investigate the effect of comonomer composition (w/w) in the feed (BuA/St) on conversion. The particle diameter (Dp), particle number (Np), hydrolysis of the IPMBI’s isocyanate groups, stability and storage properties. Films were created in which the effect of comonomer composition was also investigated, the glass transition temperature (Tg) was determined, the gel content was selected, and the mechanical properties and capacity as a barrier to water vapour and oxygen were assessed. Another series of reactions, on the other hand, was carried out in which the effect of the IPMBI content (1.5, 3.5, and 5.5%) was studied using a constant composition BuA/St=60/40. The studies conducted are the same as those mentioned for the effect of comonomer composition in feed. With increasing IPMBI percentage, slight differences in conversion, Dp, and Np were observed; the latices undergo hydrolysis during polymerization and storage. Colloidal stability, on the other hand, was preserved. The mechanical properties of the films improved as the percentage of IPMBI increased, while the boundary characteristics to moisture and oxygen improved when compared to the white film (0% of IPMBI). Finally, the formulation of a self-crosslinking water-based coating at room temperature using the previously obtained latex as a binder is reported; the coating demonstrated excellent adhesion, scratch hardness, and gloss regardless of the type of latex used.
An Overview of Arachidonic Acid Metabolic Pathway and Recent Updates on a Few Heterocyclic Derivatives showing COX-2 Inhibition
Authors: Sahoo Swagatam , Bhattacharya Ragghee , Yadav Manish , Panda Debasis Sai , Guria Tunul , Mishra Kirtimaya
Published: October 2023

Inflammation crucial part of the immune response associated with a broad range of immunological diseases. The arachidonic acid (AA) pathway, which is an essential inflammatory mediator present at the internal surface of the cellular matrix, is hydrolyzed by phospholipase A2, which leads to the development of metabolites such as cytochrome P450 (CYP), lipoxygenases (LOXs), cyclooxygenases (Coxs) and enzymes which further develops into bioactive mediators such as prostanoids, leukotrienes (LTs) and more. Cyclooxygenases produce prostaglandins, available as 2 isomorphs, COX-1(constitutive) & COX-2(inducible), targeted by NSAIDs used to treat inflammation. Yet, they have a number of negative effects that lead to market withdrawal. Research has been done to look at novel COX-2 inhibitors and safety precautions. By structural alteration at COX-2 strong receiving site, structural along with functional research on a number of selective COX-2 blockers results in the creation of novel structures that are more potent and selective against inflammation while having a very low risk of side effects. This is made possible by computer-assisted medication design. This review gives an explanation regarding the biological functionalization of several COX-2 derivatives obtained by the help of in vitro, in vivo & molecular docking for better understanding of the structures and bonding accountable for an action
Characterization of Degradation Products of Bisoprolol by LC-MS and In-silico Determination of Toxicity and Biological Activity Prediction of its Degradation Product: A Comprehensive Approach during Drug Development
Authors: Chandramore Kavita , Sonawane Sandeep
Published: October 2023

The stability of bisoprolol in tablet and bulk form was developed and validated using chromatographic methods. The bisoprolol was exposed to oxidizing agents like hydrogen peroxide, heat (dry and wet) and photolytic conditions, acid, alkali, and water hydrolysis. However, considerable degradation was observed in acid, alkali, oxidative, and wet heat thermal conditions. The drug’s stability under photolytic and dry heat conditions was established. The drug and its degradation products were separated on ODS C-18 with the specification of (250 mm × 4.6 mm, 5 μm) and the mobile phase containing acetonitrile and phosphate buffer (20 mM, pH 8) (60:40, v/v). According to ICH Q2 (R1) all method validation parameters are verified. Determination of the active pharmaceutical ingredient was not interfered by excipients or degradation products. In the 6–14 μg/mL range, the response was shown to be linear. One degradation product’s LC-MS m/z values and fragmentation patterns matched an impurity mentioned in the drug’s European Pharmacopoeia monograph. The remaining three were unidentified degradation products. LC-MS fragmentation experiments were used to characterize the products. The findings may lead to the suggestion of a more thorough drug degradation mechanism. The Toxtree software (Version 3.1.1) was then used to forecast the toxicity of the degradation products. Additional toxicity determination was carried out by using in-silico method.
A Correlative Dissolution Study of Oral Drug Delivery Systems through Reverse Phase-HPLC Analysis
Authors: Poongothai S , Srinivasan R , Rama M
Published: October 2023

Oral drug administration is essential for treating diverse diseases due to its acceptable route, non-invasiveness, minimal off-target side effects, versatility, and painless patient delivery. However, multiple drug accessibility is increasing currently but there is often a deficiency of indication in developing procedures with assessment of two diverse medicinal formulations. Oral administration of a novel antiepileptic drug, levetiracetam in grouping with either L-dopa unaccompanied or L-dopa/Ropinirole treatments was associated with dyskinesia. Altered shooting patterns of neurons relating to a compulsive harmonization process may back to the origin of dyskinesia. The current study aims to develop a dissolution technique and validate the antiparkinsonian drug-ropinirole and antiepileptic drug-levetiracetam tablets by RP-HPLC, which plays a major part in pharmaceutical formulations. The optimal dissolution conditions were experienced for the products respective to the pharmaceutical formulation and applied to assess the dissolution profiles. Ideal settings for the dissolution method were 500 mL of pH of 4.0 citrate buffer, 50 rpm and 250 nm for ropinirole tablets and 900 mL of distilled water, the rotation speed of paddle 50 rpm and 217 nm for levetiracetam tablets, then 3.84 and 3.87 minutes were set as the retention time of ropinirole and levetiracetam tablets, respectively. Technique optimization and validation process helps to reduce the bioequivalence of both dosage forms. The current study ensues detailed information on the drug release, even if there is a change in pH medium, filter, rpm, instrumentation, etc. Focusing on current pharmaceutical sciences, the proposed correlative dissolution study coupled with RP-HPLC analysis could offer a holistic insight into the performance of future oral drug delivery systems
Dasatinib and Hesperidin Loaded Nano Formulation and Preclinical Evaluation for Anticancer Activity
Authors: . Moinuddin , Neekhra Sachin , Swarnkar SK , Gupta Puneet , Gupta Deepa , Khunteta Alok , Kaushik Ujjwal , Ahmad Saeem
Published: October 2023

In this study, we aimed to develop Dasatinib/hesperidin-loaded-SLNs for chronic myeloid leukemia (CML). Utilizing a high-shear homogenizer for the synthesis, “central composite design (CCD)” was used to enhance the dasatinib/hesperidin loaded-SLNs. The optimized SLNs had PDI, particle size, and average entrapment efficiency of 0.12%, 162.3 nm, and 93%, respectively. Therefore, by enhancing the total amount of Compritol as well as sonication time the polydispersity was increased. Poloxamer 188 content had a significant influence in decreasing the polydispersity index and the entrapment efficiency (EE) of the SLN was found to be 93%. Through TEM, SEM, FTIR, DSC, and HPLC analysis, SLNs were characterized, and their anticancer efficiency was assessed in both in-vitro and in-vitro cell viability tests (MTT). SLNs containing dasatinib and hesperidin have rounded and spherical shape having a diameter of 200 nm. DSC and FTIR tests showed compatibility between the drugs and excipients. The drug release from optimized SLN formulation was under observation for 48 hours. It was found that the medication released its drug components over a protracted period of time, with 30% of the drug being released in the first four hours and the remainder 76% in the next 48 hours. According to the IC50 values determined by an independent study, dasatinib, hesperidin, and SLN were 33.97, 5158, and 4.03 μg/mL, respectively. A comparison of SLN and free drugs revealed that SLN was more effective at cytotoxicity. In this study, dasatinib and hesperidin-loaded SLNs for chronic myeloid leukemia (CML) against HL60 human leukemia cell lines were prepared and evaluated using a novel formulation approach free of toxic excipients.
Simultaneous Estimation for Diosgenin, Charantin and Hydroxychalcone from Herbal Antidiabetic Formulation using Validated HPTLC Method
Authors: Naik Archana , Gadgoli Chhaya , Patil Nikita , Salunkhe Ruchira
Published: October 2023

Most antidiabetic polyherbal preparations contain Trigonella foenum-graecum, Momordica charantia and Cinnamomum zeylanicum as active components. Although all ingredients are popular as antidiabetic agents, but the analytical method is not available for simultaneous estimation of marker compounds from herbal formulations due to several challenges. Hence, the HPTLC method was developed for simultaneous estimation of three active phytoconstituents viz. diosgenin, charantin and hydroxychalcone in polyherbal formulation. The stationary phase of the optimized HPTLC method is silica gel 60 GF254 and the mobile phase is chloroform: glacial acetic acid: methanol: water (4:3:2:1v/v). The Rf value of phytoconstituents charantin, diosgenin, and hydroxychalcone was found to be 0.72, 0.61 and 0.30 at detection wavelength 342 nm. According to ICH criteria, the analytical method validation was performed. All three markers showed linear and proportional responses in the 400 to 1400 ng/band range, which confirmed the markers’ linearity. Precision measurements were made at the intraday and interday levels, and the results were satisfactory and in line with the specifications. Accuracy was determined by the recovery method and %recovery was found to be in the range of 85.20 to 97.19. The validated HPTLC method was utilized to analyze the marketed Quanto Diab Forte capsule formulation containing charantin, diosgenin, and hydroxychalcone. The proposed validated analytical method was found to be simple, precise and accurate.
To Synthesis and Characterization of Novel 1,3,4-Oxadiazinoindole Derivatives for the Purpose of Antidepressant Activity
Authors: Agarwal Mahadev , Singla Neelam , Singh S.K
Published: October 2023

The present paper is based on the combination of MAOI hydrazide moiety and tricyclic antidepressant moiety. This combination investigates new compounds as 1,3,4-oxadiazinoindole derivatives to find potent and safer antidepressants. Synthesis of 1,3,4-oxadiazinoindole derivatives are started from various animoacids. The sequence of synthesis reactions such as benzoylation reaction, Halo-De-hydroxylation (Nucleophilic substitution reaction), Schotten-Baumann reaction, Nucleophilic addition and finally cyclization reaction (Cyclo-De-Hydroxylation) are involved. Synthesized compounds are characterized via melting point, TLC, FT infrared spectroscopy, 1H-NMR, and mass spectroscopy techniques. All compound’s structures are confirmed.
Nutritional Composition, Capsaicin Content and Enzyme Inhibitory Activities from “Bang Chang” Thai Cultivar Chili Pepper (Capsicum annuum Var. acuminatum) after Drying Process
Authors: Petcharaporn Kanyapat , Thongkao Kanittada , Thongmuang Pimporn , Sudjaroen Yuttana
Published: October 2023

Chili pepper cultivars come in a broad variety of tastes, colors, sizes, and spiciness levels due to various growth conditions. The significance of different chili peppers and other spices in Thai cuisine may be seen in dishes. This study evaluated the nutritional composition and capsaicin content from “Bang Chang” Thai cultivar chili pepper after drying, and the inhibition of lipase, tyrosinase and elastase enzymes from its oil and ethanol extracts. The nutritional composition of sun-dried chili pepper was high dietary fiber and beta-carotene, which were significantly high amount portion of Thai recommended dietary intake (RDI). According to calculations and definitions, “Bang Chang chili pepper” is a non-pungent Capsicum (0-700 SHU) since it contains a low amount of capsaicin. Total phenolic content was more abundant in ethanol extract (2.50 ± 0.13 mg GAE/g) than oil extract (1.05 ± 0.05 mg GAE/g). Compared to the positive control orlistat (IC50 = 3.26 ± 0.28 mg/mL), an anti-lipase drug, ethanol extract was 2.0 times more lipase inhibitory. In addition, ethanol extract was mildly anti-tyrosinase, while oil extract was non-activity. The anti-lipase activity of ethanol extract was due to phenolic content rather than capsaicin contained and this enzyme inhibition may act on active site. We found that ethanol extract of “Bang Chang” cultivar chili pepper yielded higher TPC content and more effective anti-lipase than oil extract. The finding provided benefits on applying this chili pepper on anti-lipase use and weight management.