bluuebunny/crossref_metadata_2025 huggingface dataset:
LA LÓGICA DIFUSA EN LA PLANEACIÓN DE LA CAPACITACIÓN
Authors: Ávila Carreón Fernando , Galeana Figueroa Evaristo
Published: February 2025

En este trabajo se presenta un análisis que proporciona una mejor toma de decisiones en un entorno de incertidumbre, dentro de la organización en el área de gestión de los recursos humanos. En este proceso se busca minimizar l a incertidumbre, mediante el uso de criterios, cuantitativos y cualitativos. Haciendo uso de fuzzy logic se toma como criterios de cálculo, las distancias Hamming y Euclidiana para determinar la matriz de semejanzas entre el grupo de individuos que se van a capacitar, encon­trando aquellos que son más afines en aquellas características o atributos que les son de interés a la organización para la mejor realización de sus tareas. Esto promueve una toma de decisiones más eficiente y eficaz, en el proceso de capacitación, lo que llevará a las empresas a una posición competitiva.
LA POLÍTICA SOCIAL EN MÉXICO, 1970-2013
Authors: Leco Tomás Casimiro , Bollain Parra Esteban
Published: February 2025

El propósito del presente artículo tiene como finalidad hacer un análisis de la política social en México, y específicamente, de los programas sociales de combate a la pobreza que se han instrumentado en este país a partir de la década de los setenta. Para contextualizar el análisis, el trabajo se inicia retomando las acciones realizadas por los gobiernos para crear las bases de la seguridad social en México durante las décadas posteriores a la Revolución Mexicana. Posteriormente, se analizan los programas sociales de combate a la pobreza en México en la etapa 1970-2013, que se derivan de la política social aplicada en el país durante ese periodo. En el artículo, se examina el origen y los objetivos de los programas sociales, y de sus cambios y variantes, terminando con un análisis general de los resultados obtenidos por estos programas. Se argumenta que mientras exista un mo­delo de organización económica y social que genere una muy acentuada desigualdad del ingreso, la población en estado de pobreza no se reducirá. Para lograr tal fin, es necesario tener como contraparte un sistema de salud, de empleo y seguridad social (Estado benefactor o un sistema de seguridad social universal), e instrumentar otras medidas que fomenten el desarrollo económico del país. Contrariamente a lo esperado, la pobreza en México no sólo sigue persistiendo, peor aún, se ha incrementado bajo el actual modelo de crecimiento económico. Ello muestra claramente el fracaso de los programas de combate a la pobreza en las últimas cuatro décadas, po­niendo en evidencia la manipulación política de la cual han sido objeto los programas sociales en México por parte de los partidos políticos.
POLÍTICAS PÚBLICAS PARA EL DESARROLLO REGIONAL DE LAS ARTESANÍAS
Authors: Duarte Duarte Rómulo
Published: February 2025

La actividad artesanal en México, es un sector que genera un importante número de empleos en casi todas las regiones del país. Sin embargo, es un sector que debido a sus características con las que produce y ante la falta de una política pública regional “desde abajo”, padece de una compleja problemática y por lo tanto, se ha quedado estancado. Debe tomarse en cuenta que la actividad artesanal, no solamente goza de un amplio reco­nocimiento cultural, sino que también tiene gran relevancia en aspectos económicos y sociales, situación que nos obliga a que dicho sector tiene que ser revalorado e impulsado con políticas públicas promovidas sobre todo por el Estado, para que se aprovechen sus capacidades productivas con la finalidad de hacer más competitivos a los artesanos.2 Así, la presente propuesta surge de la necesidad de proponer una estrategia de política pú­blica regional desde la perspectiva del desarrollo regional para el impulso del desarrollo de las artesanías en México.
CULTIVO Y COSTOS DE UN SISTEMA SILVOPASTORIL INTENSIVO (SSPI) A BASE DE GRAMINEAS Y LEUCAENA LEUCOCEPHALA ESTUDIO DE CASO EN TEPALCATEPEC, MICHOACÁN, MÉXICO
Authors: González Pérez José Manuel , Alcaraz Vera Jorge Víctor
Published: February 2025

En este trabajo se detallan los costos de establecimiento y mantenimiento de un SSPi actual a base de gramíneas y Leucaena leucocephala, compa­rándolos el sistema tradicional que operaban antes en un predio de 53 ha ubicado en Tepalcatepec, Michoacán, México con un 40% de la superficie de pasto Tanzania Panicum maximum, un 30% se sembraba sorgo forra-jero Sorghum vulgare, y el otro 30% se utilizaba en agostadero con pastos nativos, encontrando que el costo del primer año del SSPi por ha-1 es más elevado que cualquier otros cultivo; sin embargo el mantenimiento para el segundo año y subsecuentes es más bajo que el pasto T. Panicum maximum y que el cultivo de S. vulgare. Con el sistema tradicional la CCA en todo el predio era de 110.8 UA, mientras que con el SSPi subió a 291.5 UA, lo que representó un incremento del 263.4% y por ende 2.6 veces más pro­ducción de carne y leche. El detallar los costos unitarios del SSPi permite a los tomadores de decisiones tener una base económica y prever los recursos para su operación, además puede ser una herramienta auxiliar para desa­rrollar innovación de costos. Actualmente en el predio el SSPi demostró ser más rentable y sustentable que cualquier otro cultivo tradicional.
CRISIS AMBIENTAL Y LA RUTA DEL DESARROLLO SUSTENTABLE: 40 AÑOS DESPUÉS DEL SEGUNDO INFORME DEL CLUB DE ROMA
Authors: Ortiz Paniagua Carlos Francisco
Published: February 2025

Luego de celebrar el 40 aniversario del día mundial del medio ambien­te con el lema: “piensa, aliméntate y ahorra”, el Programa de Naciones Unidas para el Desarrollo, plantea entre sus propósitos la reducción de la huella ecológica. La conmemoración de ese día en especial nos lleva a la reflexión sobre la actual crisis ambiental y con ello cuál ha sido el avance en la solución a la crisis ambiental, tema de discusión en este artículo.
LA EVOLUCIÓN DE LA TELECOMUNICACIÓN HACIA UNA SOCIEDAD CONECTADA
Authors: Tovar Gil Salvador Alejandro , Lee Kim Hyun Sook
Published: February 2025

La evolución rápida de las telecomunicaciones hacia las Tecnologías de la Información y Comunicación (TIC) en los años 2000s y su aplicación ha generado una serie de términos nuevos y algunas empresas están ofrecien­do productos novedosos como dispositivos móviles para el cuidado de la salud, el servicio en línea para la supervisión del uso de energía del hogar, etc. A nivel mundial, algunos países son líderes en tanto el desarrollo de las TIC. La República de Corea, seguida por Suecia, continúa a la cabeza de la evolución de las TIC en el mundo. Durante los años 2000, existen ciertas tendencias dentro de las TIC, que están abriendo el camino hacia la Sociedad Conectada, cada una de ellas ha tomado fuerza dentro de los distintos sectores, tanto en lo social como en los negocios. Reconocien­do el fenómeno hacia una Sociedad Conectada, es recomendable que las empresas estén preparadas con el fin de conservar sus mercados actuales y conquistar futuros mercados con un perfil tecnológico
PROBLEMAS DE LA CONCIENCIA EXPORTADORA EN LA PRIMERA ETAPA DEL PROCESO DE INTERNACIONALIZACIÓN DE LAS EMPRESAS
Authors: Flores Romero Beatriz , Merlos García José Jesús
Published: February 2025

Esta investigación es acerca de los problemas de la conciencia exportadora en la primera etapa del proceso de internacionalización que afectan a las Pymes manufactureras en el estado de Michoacán. Reid (1981) denominó en su modelo de internacionalización de las empresas a la primera etapa como conciencia exportadora que es cuando el titular de la empresa de­tecta una oportunidad o un problema para incursionar en los negocios in­ternacionales, así también existen problemas de la conciencia exportadora que inhiben a las empresas a exportar. Los resultados sugieren que la falta de conocimiento de los mercados internacionales y la falta de información sobre las exportaciones son los factores de la conciencia exportadora en la primera etapa del proceso de internacionalización que afectan a las Pymes manufactureras en el estado de Michoacán encuestadas.
Evaluación del bootstrapping en la competitividad de las empresas exportadoras
Authors: Bonales Valencia Joel , Zamora Torres América Ivonne
Published: February 2025

This investigation article presents the result of a scientic research carried out to the Exporting Companies of theAgricultural Sector in the state of Michoacan. Its general objective is to determine the interrelationships betweenthe critical variables that dene the International Competitiveness of companies that export agriculturalproducts to the United States market, located in the state of Michoacan. A theoretical review was made, whichidentied the variables -quality, price, training, indices, and indicators that were integrated into a questionnairecomposed of 38 items and applied to the identied exporting companies in the sector. Once the information wasprocessed, different statistical techniques were used, and with the results obtained a Structural Model wasidentied that describes how these variables are interrelated, based on the Partial Least Square ModelingStatistical Technique (PLS) and the Bootstrapping model. After applying the questionnaire to agriculturalexporting companies, the processing of the data of each of the surveyed companies was continued, throughparametric statistics and the application of variance correlation.
Spontaneous Bone Regeneration after Traumatic Bone Loss in Young Patient: A Case Report
Authors: S Barreca , D Lacquaniti , G Calabrò , FM Scordino , A Morena , M Ventra , S Macheda , M Tescione , A Piccolo
Published: February 2025

Bone loss represents both clinical and technical challenge for orthopedic surgeons. There are several surgical options for the treatment of these patients, but currently no specific guidelines on the best management of these injuries are available. We present the case of a young male patient (12 years old) diagnosed with an open distal tibial fracture with bone loss (about 8 centimeters), a closed distal fibula fracture and posterior tibial nerve and posterior tibial arterial injuries, due to a high energy road trauma. The orthopedic surgery team scheduled tissue debridement and nerve repair, followed by the positioning of a monoaxial external fixator. Surprisingly, an x-ray performed 30 days after the surgical treatment showed a spontaneous improvement in terms of bone regeneration. Orthopedic surgeons decided to have a “wait and see approach”. A clinical follow up was scheduled in the following months and, after a year, bone was completely regenerated.
Trabalho Escravo Contemporâneo
Authors: Andrade Shirley Silveira , Andrade Sayonara Hallin Martins , Moura Mayra Santos
Published: February 2025

Este resumo expandido tem como objetivo apresentar as discussões e os resultados oriundos do projeto de pesquisa "Trabalho Escravo Contemporâneo: os impactos da pandemia da COVID-19 nas condições de trabalho das empregadas domésticas". Dessa forma, aqui, assim como na referida pesquisa, analisamos as condições do trabalho doméstico, questionando se essas condições se caracterizam como Trabalho Escravizado Contemporâneo (TEC). Utilizando técnicas de entrevistas, observação participante e pesquisa bibliográfica, foram entrevistadas 11 trabalhadoras domésticas, abordando suas histórias de vida. A análise focaliza a relação entre reprodução e produção, buscando compreender como a invisibilidade no trabalho doméstico, intrinsecamente ligada a questões de gênero, classe e raça, perpetua a degradação humana de forma natural e invisível. Não somente analisamos o artigo 149 do Código Penal do Brasil (CPB), em que está definido o crime de reduzir alguém à condição de escravizado, como também buscamos na Teoria da Reprodução Social (TRS) uma explicação para os questionamentos sobre como a invisibilização do trabalho doméstico (reprodutivo), que favorece o trabalho escravizado contemporâneo, é benéfica ao Capital, levando à exaustão desses corpos. Assim, no presente resumo expandido será possível apresentar, de forma sucinta, a correlação entre escravização contemporâneo, trabalho doméstico e perpetuação do Capital partir de uma análise legal, que demonstra a necessidade de uma interpretação do artigo 149 do CPB a partir do tipo de trabalho estudado tendo em vista as particularidades de cada labor, e teórica, na medida em que utilizamos a TRS para compreendermos como a exploração desse labor favorece a manutenção do Capital.
Reflexões sobre a insuficiência de estratégias de salvaguarda de um bem Imaterial patrimonializado
Authors: Macena Junior Ibernon Alves de , Silva Daniella Pereira de Souza
Published: February 2025

No ano de 2018, as bonecas de pano de Nossa Senhora das Dores ganharam destaque ao serem declaradas como patrimônio imaterial do município. Para nós, o ato de patrimonializar implica também, mas não apenas, uma maneira de institucionalizar o bem cultural para resguardá-lo e protegê-lo com o intuito de mantê-lo vivo na sociedade. A ação de dar a algo o status de patrimônio, é importante por fortalecer as conexões com a nossas histórias e memórias. Por esta razão, o objetivo desta pesquisa é refletir se as estratégias de salvaguarda adotadas como consequência do processo de patrimonialização, até o presente momento, podem ser consideradas suficientes para fortalecer e difundir este patrimônio imaterial junto aos moradores de Nossa Senhora das Dores. Além do levantamento bibliográfico e documental, também foram coletados dados por meio de entrevistas com gestores públicos envolvidos tanto no processo de patrimonialização quanto nos esforços de proposição das iniciativas de salvaguarda. Constatamos que a patrimonialização das bonecas de pano, para além da riqueza de significados culturais existentes, ocorreu não apenas à revelia das etapas que orientam o processo de patrimonialização adotadas pelo IPHAN, uma vez constatada a ausência de um protocolo municipal próprio de patrimonialização, como, principalmente, não propôs a contento ações efetivas de salvaguarda do bem patrimonializado. Isto posto, destacamos a importância das contribuições aqui trazidas na intenção de colaborar com o alcance do principal objetivo da patrimonialização, que é a valorização, o pertencimento e a apropriação do bem pelos seus moradores.
Educação empreendedora nos cursos profissionalizantes
Authors: Santos Whendel Whesley Segundo dos , Temóteo Tércia Leocádio , Araújo Gracyanne Freire de
Published: February 2025

A educação profissionalizante tem demandado uma preocupação no sistema educacional brasileiro a fim atender diversos problemas que afetam a inserção dos indivíduos no mercado de trabalho, a exemplo do desemprego. Diversos cursos técnicos surgem para capacitar profissionais para empreenderem. Essa pesquisa tem como objetivo compreender como a educação empreendedora contribui para a formação profissional dos estudantes de cursos profissionalizantes em Administração da cidade de Aracaju, Sergipe. O presente estudo classifica-se em qualitativo e exploratório, utilizando-se de uma análise bibliográfica para seu desenvolvimento. Os resultados obtidos informam que a educação empreendedora nos cursos técnicos se apresenta como uma ferramenta de mudança, que fortalece a perfil empreendedor dos alunos e desenvolve habilidades como iniciativa, criatividade, planejamento e competitividade. A partir desses resultados podemos concluir que essa proposta de pesquisa é relevante no campo da educação profissional ao contribuir com elementos relacionados à intenção empreendedora dos estudantes, suprindo uma lacuna na literatura sobre o ensino de empreendedorismo em cursos técnicos e seus benefícios para o mercado de trabalho.
Organizar no Antropoceno
Authors: Alves Arthur de Araujo , Lima Glessia Silva de
Published: February 2025

O presente estudo é uma pesquisa de caráter exploratório que investiga como se organiza um grupo de etnia Kariri-Xocó em Porto Real do Alagoas – AL. Diante da hegemonia eurocêntrica de conhecimento e do contexto colonial da formação do Brasil, saberes periféricos são marginalizados e silenciados, mas existem e leem a organização sob uma perspectiva outra. O trabalho é caracterizado por um estudo de caso único e se utiliza de entrevista semiestruturada feita com uma liderança Kariri-Xocó, observação participante e fotografias como fonte de coleta de dados. Tendo como base referencial o posicionamento decolonial, o trabalho observa o entendimento de conceitos e práticas de organizar no dia-a-dia de uma comunidade indígena para concluir que tais saberes são fruto de inúmeros processos adaptativos para a sobrevivência e legitimação da identidade do povo Kariri-Xocó e são fundamentados na cosmologia, tendo como grande influência as relações espirituais e de manutenção da natureza. Para organizar, os Kariri-Xocó entendem o território como um recurso primordial, a observância dos talentos como modo de desenvolver pessoas e a confiança e troca como forma de relacionamentos.
A lei de aprendizagem como ferramenta para redução de desigualdades no Brasil e em Sergipe
Authors: Alves Gustavo Henrique Santana , Sposato Karyna Batista , Abreu Lídia Nascimento Gusmão de , Silva Matheus de Souza
Published: February 2025

Este artigo tem por objetivo central compreender a sistemática em torno da Lei da Aprendizagem e sua aplicabilidade, levando em consideração as peculiaridades de crianças e adolescentes em Sergipe e os principais entraves para o devido cumprimento da legislação. Com o reconhecimento de novos sujeitos de direito e a constitucionalização promovida pela Constituição Federal de 1988, houve uma evolução em garantias para crianças e adolescentes, sendo promovidos diversos mecanismos no combate à exploração e a violação da dignidade desses indivíduos. Entre as ferramentas está a referida lei, objeto deste estudo, que busca, através da profissionalização, capacitar e inserir jovens no mundo do trabalho, visando reduzir desigualdades sociais. O artigo busca compreender que, apesar de suas potencialidades, ainda há resistência dos empregadores em cumprir a lei, por isso, o presente artigo busca refletir sobre seu contexto social, jurídico e histórico, avaliando sua aplicabilidade, eficácia e possíveis violações. Compreendendo tal problemática, cabe serem enfrentados os desafios e oportunidades da inserção de adolescentes e jovens no contexto brasileiro, levando em consideração a questão da informalidade e do desemprego. Por meio do método dedutivo bibliográfico, utilizando como fontes a legislação específica e a doutrina especializada, o trabalho identificou a importância da aplicação da lei de aprendizagem para o enfrentamento da exploração do trabalho infantil, a superação da informalidade e do desemprego, e por consequência a diminuição de desigualdades.
Enjeux et usages de la photogrammétrie pour le musée d’anatomie de l’Université Toulouse III
Authors: Golonka Janaïne
Published: February 2025

Les musées d'anatomie, héritages des traditions pédagogiques et scientifiques du XVIIIᵉ siècle, affrontent aujourd'hui des défis de conservation, de médiation et de reconnaissance institutionnelle. Le projet M.A.N 3D, mené à l’Université Toulouse III, a exploré l’utilisation de la photogrammétrie pour créer des doubles numériques de pièces anatomiques. En impliquant les étudiants, il a démontré l’intérêt de ces technologies pour enrichir l’enseignement, documenter les collections et faciliter leur accessibilité. Ces modèles numériques offrent des solutions innovantes pour repenser la conservation de collections sensibles et pour aborder les questions éthiques liées aux restes humains. Ce projet ouvre des pistes prometteuses pour la reconnaissance et la valorisation des musées d’anatomie, tout en questionnant l’évolution du rapport au corps et aux savoirs médicaux dans le contexte numérique contemporain.
Patrimoine et communauté universitaires
Authors: Soulier Virginie
Published: February 2025

Comment appréhender le patrimoine d’une université ? L’articleprésente le projet d’inventaire participatif mis en place à l’Université de Perpignan, une des plus jeunes universités de France et en même temps, une des plus anciennes d’Europe. Selon une approche info-communicationnelle, nous interrogeons la patrimonialité des objets contemporains du savoir. Nous expliquons pourquoi et comment nous impliquons la communauté universitaire en établissant les liens entre les notions de communauté et d’université. Il ressort, entreautres que les fabriques de la science et du patrimoine sontcorrélativement associées.
Comment patrimonialiser outils et créations issus du milieu hospitalier?
Authors: Costes Mylène
Published: February 2025

Il est ici question de la patrimonialisation des productions à caractère artistique en provenance du milieu hospitalier. Il prend appui sur les résultats de diverses recherches, conduites au cours d’une quinzaine d’années, entre 2007 et 2022, en France. À travers des exemples de collections tant anciennes que contemporaines, nous interrogeons les processus de patrimonialisation à l’œuvre, les choix et questionnements qui en découlent.
Pengaruh Tekanan Kompaksi Terhadap Densitas Dan Kekerasan AMC Diperkuat SiC Dan RHA Metode Metalurgi Serbuk
Authors: Febriany Sulis , Sukanto Sukanto , Subkhan Subkhan
Published: February 2025

In the current era, the automotive industry is growing rapidly, one of which is in the field of transportation in the manufacture of brake linings. Generally, brake linings are made of materials containing asbestos which endanger health. Therefore, a material innovation is needed to replace the role of asbestos such as aluminum matrix composites. The purpose of this study was to determine the effect of different hot compaction pressures on the density and hardness of AMC reinforced with SiC and RHA. The method used in this research is powder metallurgy method, which consists of preparation, mechanical mixing, compacting, and sintering stages. The mixing process uses a ball mill machine with ball ratio parameters of 10:1 and stirring time for 2 hours and with a rotating speed of 90 rpm. The compacting process is done by two-way pressing from a hydraulic press machine with a pressure gauge. In this process the parameters used are 5600 PSi, 6000 Psi and 6400 Psi pressure with a holding time of 15 minutes. Density testing was done with ASTM B962-17 according to Archimedes' law. Hardness testing using ASTM E110-14 with a portable brinell hardness tester. The test results show that the higher the compaction pressure, the higher the density and hardness values. With the highest density value of 1.69 g/mm3 and the highest hardness value of 39.33 HB for specimens with 6400 Psi compaction pressure parameters.
Rekayasa Komposit Matrik Aluminium Diperkuat Alumina Dan Bagasse Ash Dengan Metode Metalurgi Serbuk
Authors: Al Ghifari M Ghazi , Somawardi Somawardi , Rusdi Ahamad , Sukanto Sukanto
Published: February 2025

In general, brake linings are made using asbestos materials, but brake linings made from asbestos are very dangerous for health. Therefore, it is necessary to develop innovative environmentally friendly materials, one of which is aluminum matrix composite. This research process aims to determine the effect of differences in hot compaction pressure and sintering temperature on the density and hardness properties of recycled aluminium matrix composites reinforced with alumina and bagasse ash. The method used to make composites from aluminum is the powder metallurgy method, which includes preparation processes, powder mixing, compaction, and sintering. Mixing aluminium, alumina powder, and bagasse ash using a horizontal ball mill machine and using process parameters which include Ball Powder Weight Ratio (BPR) parameters of 10:1, the machine rotation speed of 90 rpm, and grinding time of 2 hours. Meanwhile, the hot compaction process uses a two-way pressing method with a hydraulic pump machine that has a pressure reader. The hot compaction process is carried out with a temperature of 500℃ held for 15 minutes and variations in the two-way compaction pressure used namely 5800 Psi, 6000 Psi, and 6400 Psi. Density testing was carried out by referring to Archimedes’ law with the ASTM B962-17 standard. This test resulted in good results at a pressure variation of 6400 Psi. Meanwhile, for hardness testing, the portable Brinell hardness test was used and referred to ASTM E110-14. In this test, the results were good at a pressure variation of 6400 Psi.
Pembuatan Game Edukasi Tata Krama untuk Santri TPA Baitussalam Sri Pemandang Berbasis Android
Authors: Ermunanda Eryshta Sevisca , Andriyanto Sidhiq , Afriansyah Riki
Published: February 2025

In the process of character building of early childhood, it is necessary to instill good attitudes and and be given a place to learn by utilizing technological developments in the learning process. Therefore, it is hoped that educational games can support the enthusiasm of children in learning to understand manners in accordance with Islamic law. Educational games can help educators both teachers and parents in providing manners education This research aims to develop educational games as a learning tool that suits the needs of TPA Baitussalam Sri Pemandang children. The method implemented is the Game Development Life Cycle (GDLC), designed using Unity application and Canva application. In this game there are three learning materials namely Manners, Asmaul Husna, and Prophet Stories. The game runs well and has been tested for functional features by experts. The results of application testing show that the material is in accordance with the source book used in Caberawit learning with a percentage of 95.71%. To calculate User Acceptance Testing, taken from testing to children Caberawit TPA Baitussalam Sri Pemandang get a final score of 85.9%. After doing the UAT calculation, it can be concluded that the manners educational game falls into the "Very Feasible" criteria.
A Socio-pragmatic Analysis of Jordanian University Students’ Status Updates
Authors: Orabi Ola Awni , Bader Yousef F.
Published: February 2025

Some new sources of communication such as Facebook, WhatsApp and others have contributed to the social and academic development of various individuals worldwide. This socio-pragmatic study aims to investigate types of speech acts and gender differences in language usage among university students, focusing on WhatsApp. WhatsApp is used for various purposes and allows for a wide range of vocabulary and linguistic structures in different expressive formats, including images and text. Specifically, the study purports to examine the increasing use of WhatsApp and understand its impact on social interactions and academic performance of Jordanian university students from three universities. A total of 100 WhatsApp status updates were collected to study the influence of the main factor, gender, on these updates. Subsequently, a questionnaire was distributed to a sample of 100 students to elicit their opinions regarding status updates. The study reveals that personal interests, values, and personalities exhibit significant variability, affecting how students choose to represent themselves through their status updates. Male students often emphasize their academic and professional accomplishments, while female students tend to prioritize social connections and personal experiences. Assertive, directive and expressive speech acts are found to be the most commonly used in our sample’s status updates.
Feasibility and challenges of submerged culture of steelhead trout Oncorhynchus mykiss for open ocean aquaculture in the United States
Authors: Chambers Michael , Coogan Michael , Doherty Michael , Rillahan Chris , Lader Pal , Howell Willaim H.
Published: February 2025

To meet growing seafood demands, the US aquaculture industry will need to consider farming the open ocean in a responsible manner. However, offshore environments can be energetic (seas > 8 m) making it difficult to maintain surface cage systems. To minimize potential storm damage, submerged culture technologies can be employed to safeguard the infrastructure and product. Steelhead trout (Oncorhynchus mykiss) have potential as an offshore species, though they have open air bladders (physostomous), and need access to air to inflate their swim bladders. To address this concern, three experiments were developed to explore the ability of O. mykiss to cope with extended periods of submergence. The studies used small (~300 g) and large (~1000 g) trout, in cages that ranged from 3.7 to 68 m3, that were submerged for periods of one to four weeks. Data storage tags (DST), sonar and video were used to quantify their ability to manage with submergence. Results indicated differences in growth, condition, and mortality among the treatments. The study suggests O. mykiss can be submerged for days to weeks with no negative effects, but that submergence times >3 weeks can be detrimental. It is therefore recommended that when culturing O. mykiss in areas exposed to severe storms or hazardous environmental conditions (e.g. harmful algal blooms or icing), submersible cages should be utilized to avoid these events up to 3 weeks.
Targeting aldose reductase using natural African compounds as promising agents for managing diabetic complications
Authors: Gakpey Miriam E. L. , Aidoo Shadrack A. , Jumah Toheeb A. , Hanson George , Msipa Siyabonga , Mbaoji Florence N. , Bukola Omonijo , Tjale Palesa C. , Sangare Mamadou , Tebourbi Hedia , Awe Olaitan I.
Published: February 2025

Background

Diabetes remains a leading cause of morbidity and mortality due to various complications induced by hyperglycemia. Inhibiting Aldose Reductase (AR), an enzyme that converts glucose to sorbitol, has been studied to prevent long-term diabetic consequences. Unfortunately, drugs targeting AR have demonstrated toxicity, adverse reactions, and a lack of specificity. This study aims to explore African indigenous compounds with high specificity as potential AR inhibitors for pharmacological intervention.

Methodology

A total of 7,344 compounds from the AfroDB, EANPDB, and NANPDB databases were obtained and pre-filtered using the Lipinski rule of five to generate a compound library for virtual screening against the Aldose Reductase. The top 20 compounds with the highest binding affinity were selected. Subsequently, in silico analyses such as protein-ligand interaction, physicochemical and pharmacokinetic profiling (ADMET), and molecular dynamics simulation coupled with binding free energy calculations were performed to identify lead compounds with high binding affinity and low toxicity.

Results

Five natural compounds, namely, (+)-pipoxide, Zinc000095485961, Naamidine A, (−)-pipoxide, and 1,6-di-o-p-hydroxybenzoyl-beta-d-glucopyranoside, were identified as potential inhibitors of aldose reductase. Molecular docking results showed that these compounds exhibited binding energies ranging from −12.3 to −10.7 kcal/mol, which were better than the standard inhibitors (zopolrestat, epalrestat, IDD594, tolrestat, and sorbinil) used in this study. The ADMET and protein-ligand interaction results revealed that these compounds interacted with key inhibiting residues through hydrogen and hydrophobic interactions and demonstrated favorable pharmacological and low toxicity profiles. Prediction of biological activity highlighted Zinc000095485961 and 1,6-di-o-p-hydroxybenzoyl-beta-d-glucopyranoside as having significant inhibitory activity against aldose reductase. Molecular dynamics simulations and MM-PBSA analysis confirmed that the compounds bound to AR exhibited high stability and less conformational change to the AR-inhibitor complex.

Conclusion

This study highlighted the potential inhibitory activity of 5 compounds that belong to the African region: (+)-Pipoxide, Zinc000095485961, Naamidine A, (−)-Pipoxide, and 1,6-di-o-p-hydroxybenzoyl-beta-d-glucopyranoside. These molecules inhibiting the aldose reductase, the key enzyme of the polyol pathway, can be developed as therapeutic agents to manage diabetic complications. However, we recommend in vitro and in vivo studies to confirm our findings.
Distribution and frequency of genetic mutations in three insecticide targets in field populations of Culex tritaeniorhynchus in Mianyang City, Sichuan Province, China
Authors: Xie Hongwei , Tang Meilin , Sun Hongying , Huang Zhengzheng , Dong Mengmeng , Wen Xianying
Published: February 2025

Japanese encephalitis (JE) is an important mosquito borne infectious disease which is mainly transmitted by Culex tritaeniorhynchus Giles (1901) in China. At present, vector control remains an important means to prevent and control vector-borne diseases including JE. The development of insecticide resistance has seriously threatened the effectiveness of insecticide-based vector control programmes. Therefore, understanding insecticide resistance in the target pest is essential to inform evidence-based vector control. In Mianyang City of Sichuan Province of China, Cx. tritaeniorhynchus is the dominant mosquito species, and JE has been documented in this city. Unfortunately, there is little information on the status and underlying mechanisms of insecticide resistance in field populations of Cx. tritaeniorhynchus, the main JE vector in this region. In the study, a total of 314 adults of Cx. tritaeniorhynchus, collected from 6 sites across Mianyang City, were inspected for resistance-conferring mutations in three genes encoding voltage-gated sodium channel (VGSC), Gamma-aminobutyric acid (GABA) receptor Rdl subunit (Rdl), and acetylcholinesterase (AChE) by DNA Sanger sequencing. The results showed that the classical L1014F mutation in VGSC was distributed in all the 6 populations at varying frequencies from 16.98% to 27.78%, and the frequency of F455W mutation in AChE was extremely high (97.06%-100%). Notably, the conserved mutations A296S and V327I previously reported in the Rdl of some other species of mosquitoes were discovered in Cx. tritaeniorhynchus for the first time. The frequency of the resistant Rdl 296S allele was 62.04% to 94.00%, while the V327I mutation was present at a much lower frequency ranging from 0.93% to 1.8%. Overall, the prevalent co-existence of resistance-conferring mutations in multiple insecticide target proteins in Cx. tritaeniorhynchus populations in Mianyang City indicates a worrying status of insecticide resistance, and suggests that it is highly required to monitor the phenotypic resistance of Cx. tritaeniorhynchus on a regular basis.
Case Report: A case of transcatheter aortic valve replacement in a patient with a small aortic annulus using the Acurate Neo2
Authors: Malik Shahzil Abdur Rehman , Ali Jamshed , Javed Maleeha , Hussain Kamran , Azeem Navaira , Rehman Nasir , Faheem Osman
Published: February 2025

Small aortic annulus poses a major challenge in aortic valve replacement due to the increased risk of prosthesis–patient mismatch (PPM) and increased surgical risk. In recent years, transcatheter aortic valve replacement (TAVR) has emerged as a popular alternative to the traditional surgical aortic valve replacement. We present the case of an 80-year-old woman with a small aortic annulus who underwent TAVR using a self-expanding transcatheter heart valve Acurate Neo2 after an non-ST-segment elevation myocardial infarction (NSTEMI) presentation. These risks, combined with advanced age, significant co-morbidities, and a severely calcified small aortic annulus, supported the choice of TAVR with a self-expanding Acurate Neo2 valve. Despite multiple risk factors for PPM, the patient had a successful outcome without major complications. Our case highlights the off-label use of the Acurate Neo2 valve in one of the smallest aortic annuli reported to date, showcasing its feasibility in Asian and resource-limited settings.
Correlation analysis of Hashimoto’s thyroiditis with papillary thyroid carcinoma occurrence and its central lymph node metastasis: a single center experience
Authors: Sun Kang , Wang Xiaoming , Chen Dexuan , Ma Chaoqun
Published: February 2025

Purpose

This study investigates the clinicopathological characteristics of papillary thyroid carcinoma (PTC) with coexisting Hashimoto’s thyroiditis (HT) and further explores the risk factors for central lymph node metastasis (CLNM) in PTC.

Method

A retrospective analysis was conducted on 415 PTC patients who underwent surgical treatment for thyroid cancer at the First Affiliated Hospital of Anhui University of Chinese Medicine from 2016 to 2022. Clinicopathological features were compared between PTC patients with and without HT. Univariate and multivariate logistic regression were used to analyze the risk factors of CLNM.

Result

The PTC+HT group had a higher proportion of female patients (85.5%) than the PTC group (P<0.05). Univariate analysis revealed no statistically significant difference between the two groups in eight aspects (all P>0.05). Multivariate analysis showed that HT was positively associated with the total number of central lymph node (CLN) dissected, Thyroid-stimulating hormone (TSH), Thyroid peroxidase antibody (TPOAb), and Thyroglobulin antibodies (TgAb), while identified as a protective factor against invasion with an odds ratio of 0.422 (95%CI 0.209-0.853, P=0.016). Through univariate and multivariate logistic regression, we proved that tumor position, Capsule + Extrathyroidal extension (ETE), multifocal tumors, and the total number of CLN dissected were independent risk factors for CLNM. Multiple linear regression analysis told us that invasion (β= 0.093, p=0.048) had a positively predictive impact on CLN positive rate.

Conclusion

Female PTC patients are more prone to concurrent HT, which elevates the levels of TSH, TPOAb, and TgAb. HT not only promotes the longitudinal growth of nodules and PTC development, but also reduces the risk of invasion and CLNM. Therefore, we posit that the impact of HT on PTC patients is a “double-edged sword”. Isthmus, Capsule + ETE, multifocality, age < 55 years old, and male are high-risk factors for CLNM in PTC, while HT is regarded as a protective factor. Capsule + ETE is the primary risk factor affecting the CLN positive rate.
HPK1 kinase inhibitor: a sufficient approach to target HPK1 to modulate T cell activation in cancer immunotherapy compared with degraders
Authors: Wang Qin , Zhu Xinyi , Li Jing , Xu Sanjia , Wang Ali , Zhang Xinwen , Wang Xingxing , Cai Xiaopeng , Xing Haimei , Liu Ye , Liu Xuesong , Wang Zhiwei , Wang Lai , Yuan Xi
Published: February 2025

Background

Hematopoietic progenitor kinase 1 (HPK1) is a member of the mitogen-activated protein kinase kinase kinase kinase (MAP4K) family. It has been reported that HPK1 negatively regulates the activation of T cells. Several compounds have been developed and tested in clinical trials to target HPK1 for cancer immunotherapy. However, whether kinase inhibition is sufficient to eliminate the immunosuppressive function of HPK1, particularly in T cells, remains elusive.

Methods

In this study, genetic tools were used to edit the human T lymphocyte cell line Jurkat. The activation of HPK1-null cells, HPK1-wildtype cells and HPK1-kinase-inactive cells was compared through ectopic expression of HPK1 in HPK1 knockout cells or direct HPK1 mutation. Besides genetic validation, a series of compounds that selectively target HPK1 (with or without HPK1-degradation activity) were used to assess the potential scaffold function of HPK1 in regulation of human primary T cell activation and cytotoxic activity.

Results and conclusion

Augmented T-cell receptor (TCR)-induced activation in HPK1-knockout Jurkat cells was inhibited by complementation of wildtype, but not kinase-dead HPK1. HPK1 K46E-knockin and K46*-knockin Jurkat cells showed comparable levels of enhanced TCR-induced activation compared with control HPK1-wildtype Jurkat cells. Similarly, HPK1 kinase inhibitor (Compound 1) and cereblon-based (CRBN-based) HPK1 degrader (Compound 2) elicited similar degrees of maximum TCR-induced activation in primary human peripheral blood T cells. In summary, the results of this study suggested that HPK1 kinase inhibitor may be sufficient for HPK1 targeting in T cell mediated cancer immunotherapy.
Ddx21 mutant peptide is an effective neoantigen in prophylactic lung cancer vaccines and activates long-term anti-tumor immunity
Authors: Zhang Zhe , Xia Yimeng , Wang Zhihong , Sun Yaxing , Pu Dan , He Yijia , Liu Ruixian , Zhang Yanru , Liu Yan , Yu Junzhi , Ning Shiyang , Feng Baisui , Wang Yaohe , Wang Na
Published: February 2025

Introduction

Lung cancer is the leading cause of cancer-related death worldwide, and its morbidity and mortality are increasing. Although low-dose CT lung cancer screening has been shown to reduce lung cancer mortality, its adoption rate is limited and the pace of its promotion is slow, highlighting the urgent need for more effective prevention measures. Prophylactic vaccines play a crucial role in cancer prevention. Our previous studies indicated that mice immunized with a prophylactic vaccine based on lung cancer cell lines KPL 160302S, derived from early-stage murine lung cancer tissues, exhibited a significantly extended survival period, with a strong anti-tumor immune response. While the vaccine based on KPL 160424S, derived from advanced-stage murine lung cancer tissues, failed to extend survival time and demonstrated limited capacity to stimulate anti-tumor immunity.

Methods

To investigate the fundamental reason for the difference between KPL 160302S and KPL 160424S vaccines, we employed bioinformatics methods and immune related experiments to explore the effects and mechanisms of the screened neoantigens.

Results

Our findings demonstrated that immunization with the Ddx21 mutant peptide (Ddx21MT), unique to KPL 160302S, could significantly increase the proportion of central memory T cells (TCM) in mice and activate anti-tumor immunity.

Discussion

These results suggest that the Ddx21MT is a highly effective neoantigen that can activate anti-tumor immunity, which can serve as an important component in developing a lung cancer vaccine and is expected to be used in combination with other immunotherapy approaches.
Pre-transplant T-cell clonal analysis identifies CD8+ donor reactive clones that contribute to kidney transplant rejection
Authors: Sanders Jes M. , Banbury Barbara L. , Schumacher Erika L. , He Jie , Sambandam Yuvaraj , Fields Paul A. , Gallon Lorenzo , Mathew James M. , Leventhal Joseph R.
Published: February 2025

Introduction

Responses to allogeneic human leukocyte antigen (HLA) molecules limit the survival of transplanted organs. The changes in T-cell alloreactivity that contribute to this process, however, are not fully understood. We defined a set of donor reactive T-cell clones (DRTC) with the goal to elucidate signatures of kidney allograft rejection.

Methods

DRTC were identified pretransplant using an anti-donor mixed lymphocyte reaction assay: CFSE-diluting CD4+ and CD8+ DRTC were flow-sorted, and the TCR sequences were identified using Adaptive Immunosequencing. DRTC were then tracked in post-transplant biopsies, blood, and urine samples in a cohort of kidney transplant recipients.

Results

In patients with an abnormal biopsy, the majority of CD8+ DRTC found within the allograft were detected in the circulating pre-transplant repertoire. Circulating CD8+ DRTC were more abundant pre- and post-transplant in patients that received non-lymphodepletional induction and developed an abnormal biopsy when compared to stable patients. Additionally, DRTC were detected as early as two weeks post-transplant in the urine of some patients, with some of these clones subsequently identified in follow-up kidney biopsy samples.

Discussion

The findings of our study add to our understanding of T-cell alloreactivity following kidney transplantation and provide evidence for the role of pre-defined alloreactive T-cells in the development of allograft rejection.
Rheumatoid arthritis associated cytokines and therapeutics modulate immune checkpoint receptor expression on T cells
Authors: Emerson Dana , Merriman Eve , Yachi Pia P.
Published: February 2025

Introduction

We investigated the impact of rheumatoid arthritis (RA) associated cytokines and standard of care (SOC) RA therapeutics on immune checkpoint receptor (IR) expression on T cells to gain insights to disease pathology and therapeutic avenues.

Methods

We assessed IR expression by flow cytometry on T cell receptor activated T cells cultured in the presence of exogenously added single cytokines or RA patient synovial fluid. We also assessed RA synovial fluid stimulated samples in the presence of various single cytokine neutralizing antibodies or SOC therapeutics, including glucocorticoids, TNF, IL-6 receptor and JAK inhibitors. In addition to IR expression, we measured the impact on cytokine secretion profiles.

Results

RA-associated cytokines modulated IR expression, suggesting a role for these cytokines in regulation of disease pathology. By dissecting the influence of various inflammatory drivers within the RA inflammatory milieu, we discovered distinct regulation of IR expression by various cytokines including IL-10, IFNα/β, and TNF. Specifically, increased expression of TIM-3, PD-1, LAG-3 and CD28 in response to RA synovial fluid was driven by key cytokines including IL-6, IL-10, IL-12, IFNs, and TNF. In addition, SOC RA therapeutics such as glucocorticoids and TNF inhibitors modulated IR and cytokine expression in the presence of RA synovial fluid.

Conclusions

This study points to an important and intricate relationship between cytokines and IRs in shaping immune responses in autoimmune pathology. The modulation of IR expression by RA-associated cytokines and SOC therapeutics provides new insights for the use of targeted treatments in managing RA pathology.
Sepsis subphenotypes: bridging the gaps in sepsis treatment strategies
Authors: Zhang Xue , Zhang Wei , Zhang Huan , Liao Xuelian
Published: February 2025

Sepsis, a heterogeneous illness produced by a dysregulated host response to infection, remains a severe mortality risk. Recent discoveries in sepsis research have stressed phenotyping as a feasible strategy for tackling heterogeneity and enhancing therapy precision. Sepsis phenotyping has moved from traditional stratifications based on severity and prognosis to dynamic, phenotype-driven therapeutic options. This review covers recent progress in connecting sepsis subgroups to personalized treatments, with a focus on phenotype-based therapeutic predictions and decision-support systems. Despite ongoing challenges, such as standardizing phenotyping frameworks and incorporating findings into clinical practice, this topic has enormous promise. By investigating phenotypic variation in therapy responses, we hope to uncover new biomarkers and phenotype-driven therapeutic solutions, laying the groundwork for more effective therapies and, ultimately improving patient outcomes.