bluuebunny/crossref_metadata_2025 huggingface dataset:
OnDermatoxys hispaniensisn.sp. (Nematoda: Oxyuridea), fromOryctolagus cuniculusandLepus timidusof Spain
Authors: Vicente F. Simon
Published: June 2009

Dermatoxys hispaniensisn.sp., collected from the intestine of hares and wild rabbits is described, and details are given of the cephalic anatomy. As far as our knowledge goes, this is the first record ofDermatoxysin Spain and also in the leporine species of Europe.

A key for identifying the species ofDermatoxysis provided, based on the available data.
Polyonchobothrium scleropagis n. sp. (Cestoda: Pseudophyllidea) from the Australian osteoglossid fish Scleropages leichardti Günther
Authors: Blair David
Published: June 2009

Polyonchobothrium scleropagis (Cestoda: Pseudophyllidea) is described as a new species from the Australian osteoglossid fish Scleropages leichardti Günther.
Tapeworms of the Chaco Boreal, Paraguay, with two new species
Authors: Schmidt Gerald D. , Martin Robert L.
Published: June 2009

Mathevotaenia paraguayae sp. n. is described from Euphractus sexcinctus. It differs from M. didelphidis (Rud.) in having a transverse cirrus pouch anterior to the vagina. Monoecocestus mackiewiczi sp. n. was found in Phyllotis sp. It differs from M. parcitesticulatus Rego in having a much smaller scolex, a poral ovary, and a cirrus pouch 360 to 440 μm long. Other species reported are Taenia macrocystis, Taenia omissa, Spirometra mansonoides, Spirometra gracilis, Spirometra longicollis, Diphyllobothrium trinitatis, Atriotaenia parva, and Mathevotaenia tetragonocephala.
Paratrichosoma crocodilus n. gen. n. sp. (Nematoda: Trichosomoididae) from the skin of the New Guinea crocodile
Authors: Ashford R. W. , Muller Ralph
Published: June 2009

A new genus of nematode (Paratrichosoma crocodilus) is described from Crocodilus novaeguinae. Adults have typical trichuroid features but elongated stichocytes (over 1 mm in female). Males are one third to one half as long as females and have no spicule or sheath.
Immunization of sheep against Schistosoma bovis using an irradiated schistosomular vaccine
Authors: Taylor M. G. , James E. R. , Bickle Q. , Hussein M. F. , Andrews B. J. , Dobinson A. R. , Nelson G. S.
Published: June 2009

S. bovis is an economically important parasite of cattle and possibly of sheep and other domestic stock in many African and Mediterranean countries. Control of this infection by chemotherapy and mollusciciding is impractical and this has stimulated interest in vaccination. Previous work in sheep with the Central and South African species S. mattheei has led to the development of an effective vaccine which incorporates irradiated schistosomula produced by artificial transformation of cercariae. In the present experiments a similar S. bovis vaccine has been used in sheep. Fifteen 5 month old Border Leicester × Suffolk crossbred wethers were divided into three groups, two were vaccinated with either 10000 or 20000 6 krad irradiated S. bovis schistosomula, and the third kept as unvaccinated controls. All sheep were challenged with 6700 normal S. bovis cercariae.

Worm recoveries and tissue egg counts at necropsy showed that vaccination with 10000 irradiated schistosomula produced a reduction of 71% in the mean worm burden resulting from challenge of the controls and the mean densities of eggs in the tissues were reduced by 75–82%. Immunization with 20000 irradiated schistosomula was not more effective. Histopathological observations showed that both groups of vaccinated sheep had much milder lesions in the intestines than the non-vaccinated controls. There was also a clear reduction in the severity of liver lesions in 4 of the 5 sheep given the lower dose of irradiated schistosomula but the livers of sheep vaccinated with 20000 irradiated schistosomula had severe and widespread lesions due mainly to a massive accumulation of eosinophils and other inflammatory cells around the vessels.
Studies on the life cycle of Asymphylodora tincae (Modeer, 1790) (Trematoda: Monorchiidae) in a small lake near Amsterdam
Authors: van den Broek Elisabeth , de Jong Nienke
Published: June 2009

Aspects of the life cycle of Asymphylodora tincae have been studied in a small lake near Amsterdam. Bithynia tentaculata, being the only snail species that was very numerous in the lake, was the first as well as the main second intermediate host; adult trematodes were found exclusively in the tench, Tinca tinca. A small part of the cercariae that penetrate into B. tentaculata was able to develop into progenetic specimens. A morphological description is given of the following stages of A. tincae: adult, egg, redia, cercariaeum, metacercaria and progenetic stage. The cercariaeum is identified as Cercariaeum paludinae impurae De Filippi, 1854.

It is concluded that A. tincae is a species, capable of large variation regarding both morphological structure (e.g. body spination, size) and life cycle.
Prevalence of Toxocara spp. eggs in some public grounds and highway rest areas in Kansas
Authors: Dada B. J. O. , Lindquist W. D.
Published: June 2009

Out of a total of 282 soil samples obtained from public areas, 58 samples contained Toxocara spp. eggs. This gave an overall prevalence of 20.6%. Highway rest areas were contaminated and childrens sand boxes had the highest percentage of recovery of all.
Trichostrongylus vitrinus: The influence of age of sheep and population size on the intestinal distribution
Authors: Taylor S. M. , Kilpatrick D.
Published: June 2009

Two age groups of parasite-free lambs, 4 months and 8 months, were infected with 50 000 infective larvae (L3) of T. vitrinus. A further group of 8 month old lambs were infected with 250 000 L3 T. vitrinus. The lambs were slaughtered at intervals after infection, and the size and linear distribution of their intestinal worm burdens examined and compared statistically. After the 50 000 L3 infections, it was found that a higher percentage of the initial inoculum had become established in the younger lambs but that there were no differences between the intestinal distributions of the population in either age group.

After infection with 250 000 L3, the percentage of the larvae retained changed with time. Up to sixteen days post infection, 38% of the larvae administered were retained; thereafter the percentage fell to 22%. The linear distribution of the population in this size of infection showed that a significantly greater percentage was present between 4.8 and 7.2 metres distal to the pylorus when compared with that after infection with 50 000 L3.
A new nematode Neocamallanus bareilliensis from a piscine host Channa punctatus Bloch
Authors: Sharma R. K. , Sharma P. K.
Published: June 2009

Neocamallanus bareilliensis n. sp. has been described from the intestine of a freshwater fish Channa punctatus from Bareilly (U. P.) India. It has been distinguished from the other three species of the genus reported so far.
A description of adult Wuchereria bancrofti (rural strain) from an experimental infection in the long-tailed macaque, Macaca fascicularis (Syn. M. irus)
Authors: Dissanaike A. S. , Mak J. W.
Published: June 2009

Adult worms of the rural strain of Wuchereria bancrofti in Peninsular Malaysia obtained from a successful experimental transmission in an immunosuppressed Macaca fascicularis are described for the first time. Although the worms, especially females, were slightly smaller, they were similar in morphology to those of the periodic and non-periodic W. bancrofti previously described.
Nilocotyle duplicisphinctris sp. n. (Trematoda: Paramphistomidae) from Hippopotamus amphibius
Authors: Sey O. , Graber M.
Published: June 2009

Nilocotyle duplicisphinctris sp. n. was recovered from the stomach of Hippopotamus amphibius in Ethiopia. The average body size (L × W) is 3.6 × 1.2 mm; pharynx 0.5 × 0.3, Dicranocoelium type; other diameter of the acetabulum 0.85, Nilocotyle type; anterior testis 0.34 × 0.49; posterior testis 0.32 × 0.27; ovary 0.27 × 0.19; genital opening 0.30 × 0.34, Duplicis phinctris type and eggs 0.15 × 0.07. The most characteristic specific feature is the presence of a double sphincter along the genital atrium.
The effect of gamma irradiation on Nematospiroides dubius. Factors affecting the survival of worms in a primary infection in mice
Authors: Behnke Jerzy M. , Parish Heather A. , Hagan Paul
Published: June 2009

The infective larvae of Nematospiroides dubius were exposed to various levels (0–30 krad) of gamma irradiation by means of a Cobalt 60 source. Groups of mice were infected with these larvae and autopsied 5 weeks later for worm counts. It was found that male worms were more susceptible to irradiation than female worms. In both instances, however, the survival curve on a semi logarithmic plot was characterised by a shoulder at low doses and an exponential component at the higher levels of exposure. No male worms were recovered from mice infected with larvae given more than 12 krad but some female worms were capable of surviving 20 krad. The fecundity of female worms was reduced by 61% at 4 krad and totally ablated at 8 krad.

Further experiments demonstrated that the survival of irradiated N. dubius in vivo was related to the extent of the damage caused at the time of irradiation and was not dependant on additional host parameters. Thus neither the number of irradiated worms inoculated nor the sex of the host radically altered the sex ratio or proportion of the worms lost as a result of irradiating the larvae. Furthermore, treatment with cortisone or sublethal irradiation of the host did not increase the proportion of surviving worms. It was, therefore, concluded that a host immune response was not involved.
Opisthorchis viverrini:Partial success in adoptively transferring immunity with spleen cells and serum in the hamster
Authors: Flavell D. J. , Pattanapanyasat K. , Flavell Sopsamornu U.
Published: June 2009

Three groups of golden Syrian hamsters received intraperitoneally either, (1) 1 × 107spleen cells, (2) 0.5 ml. serum or, (3) 1 × 107spleen cells plus 0.5 ml. serum, from donors infected 6 weeks previously with 25Opisthorchis viverrinimetacercariae, one day before and at the same time as challenge with 25 metacercariae. Three groups of control animals received the same quantities of spleen cells, serum or an admixture of both from normal non-infected donors in the same manner followed by challenge with 25 metacercariae.

Animals were killed six weeks after challenge, livers and extrahepatic biliary systems carefully removed, and liver worm burdens estimated. Additionally, parasitic egg counts were performed on pooled faeces samples collected one hour prior to death.

Animals receiving spleen cells, serum, or an admixture of both, from normal non-infected donors had mean worm burdens of 11.44, 12.00 and 12.66, respectively. Animals receiving spleen cells, serum, or both, from infected donors had mean worm burdens of 9.88, 7.77 and 12.00, respectively. There were no significant differences in mean worm burdens between control and experimental groups. However, a substanial reduction in parasitic faecal egg counts and subsequently mean egg production per worm was observed in all 3 groups of animals receiving spleen cells, serum, or both, from infected donors when compared to their respective control group.

These findings are discussed in relation to adoptive transfer studies performed with other helminths and their possible relevance to naturally acquired immunity is commented on.
Castroia kamariae sp. nov. and Limatulum kuziai sp. nov. (Lecithodendriidae) from the free-tailed bat (Tadarida mops de Blainville) from West Malaysia
Authors: Palmieri James R. , Krishnasamy M. , Sullivan John T.
Published: June 2009

Thirteen bats, Tadarida mops de Blainville, collected from the Ampang district in Kuala Lumpur, Malaysia, were found positive for the trematodes Castroia kamariae sp. nov. and Limatulum kuziai sp. nov. Two distinct but morphologically similar forms of Castroia kamariae were recovered. The morphological type is apparently determined by its location in the host intestine.
Anticestode activity of 3, 5-dibromo-2' -chlorosalicylanilide-4' -isothiocyanate—a preliminary report
Authors: Gupta Suman , Katiyar J. C. , Sen A. B. , Dubey S. K. , Singh H. , Sharma S. , Iyer R. N.
Published: June 2009

Many compounds have been claimed to possess powerful taenicidal activity but only a few are able to remove the tapeworms complete with scolices; accordingly better drugs are still needed. A series of 3, 5-dibromosalicylanilides, possessing a molecular framework similar to that of niclosamide (Gönnert and Schraufstätter, 1960) was tested for taenicidal activity; the most potent anticestode compound was found to be 3, 5-dibromo-2' -chlorosalicylanilide-4' -isothiocyanate (CDRI Compound 77–6). The present communication compares the efficacy of Compound 77–6 with niclosamide, N-(2' -chloro-4-nitrophenyl)-5-chlorosalicylamid (Bayer, power with 75% active ingredient) and praziquantel, 2-cyclohexylcarbonyl-1–3, 4, 6, 7, 11 b-hexahydro-2H-Pyrazino [2–1-a], isoquinoline-4-one (Droncit, E. Merck & Bayer, powder, 100% pure) (Seubert et al., 1977) in rats and mice.
Taeniasis, cysticercosis and echinococcosis/hydatidosis in Nigeria: II — prevalence of bovine and porcine cysticercosis, and hydatid disease in slaughtered food animals based on retrospective analysis of abattoir records
Authors: Dada B. J. O.
Published: June 2009

A retrospective analysis of slaughterhouse records during the pre-veterinary-organized meat inspection era (before 1970) for cysticercosis and hydatidosis in food animals showed that parasitic cysts were documented but not identified and that the numbers of slaughtered animal species harbouring various parasitic cysts were not recorded. Since the various parasites which the meat inspectors observed were not identified, the prevalence of species encountered could not be determined.

An analysis of abattoir records during the veterinary meat inspection era showed that Cysticercus bovis and C. cellulosae infections were documented in cattle and pigs respectively. Moreover, hydatid cysts were detected in cattle, pigs, sheep, goats and camels.

The data obtained from this survey cannot be wholly relied upon as accurate, but it can be used as a baseline for the proper evaluation of the status of these zoonotic parasitic diseases in future co-ordinated researches.
Helminth parasites of wild cats in north-east Scotland
Authors: Burt M. D. B. , Pike A. W , Corbett L. K
Published: June 2009

Three cestode species, namelyTaenia taeniaeformisBatsch, 1786,Taenia pisiformis(Bloch, 1780) andMesocestoides litteratus(Batsch, 1786) and one nematode species,Toxocara catiSchrank, 1788, were found in wild cats collected in Grampian region, Scotland. Of the 41 cats examined, 25 wereFelis silvestris, 12 were feralFelis catusand four were hybrids ofF. silvestrisandF. catus.
Ascaridoid nematodes of Sirenians—the Heterocheilinae redefined
Authors: Sprent J. F. A.
Published: June 2009

The two ascaridoids of sirenians, Heterocheilus tunicatus Diesing, 1839, and Paradujardinia halicoris (Owen, 1833), are redescribed. A basic affinity between the two species is indicated in the bilateral structure of the excretory system, the globular ventriculus, long intestinal caecum, and the form of the male tail. The subfamily Heterocheilinae is redefined so as to contain these two genera. It is proposed that the subfamily comprises a natural suprageneric group within the Ascarididae sensu Hartwich, 1974 containing, in addition to Heterocheilus and Paradujardinia, the following genera occurring in freshwater, estuarine and coastal vertebrates: Multicaecum Baylis, 1923; Dujardinascaris Baylis, 1947; Gedoelstascaris Sprent, 1978; Ortleppascaris Sprent, 1978; Brevimulticaecum Mozgovoy sensu Sprent, 1979; Krefftascaris Sprent, 1980. The suggestion is made that the ascaridoids of sirenians originated from forms in fluviatile lower vertebrates.
The Ultrastructural Changes in the Abomasal Mucosa of Calves Experimentally Infected with Trichostrongylus axei
Authors: Ross J. G. , McCracken J. , Purcell D. A.
Published: June 2009

The normal ultrastructure of the fundic gastric glands of cattle is described and compared with those from calves infected with T. axei. Loss and alterations in structure of zymogen and parietal cells are observed and are related to changes observed by light microscopy. The significance of these changes is discussed.
Revision of the genera Longistrongylus Le Roux 1931, Kobusinema Ortlepp, 1963 and Bigalkenema Ortlepp, 1963 (Nematoda: Trichostrongylidae)
Authors: Gibbons Lynda M.
Published: June 2009

The species of the genera Longistrongylus, Kobusinema and Bigalkenema are redescribed. Kobusinema and Bigalkenema are considered synonyms of Longistrongylus and their species are transferred as new combinations. Bigalkenema neveulemairei (Gutteres, 1947) Jansen, 1958 and Longistrongylus muraschkinzevi (Shulz and Kadenatsii, 1950), Jansen, 1958 are considered species inquirendae. A key to the species of the genus Longistrongylus is given and their hosts and geographical localities are listed.
Studies on the mechanism of lysis ofEchinococcus granulosusprotoscoleces incubated in normal serum
Authors: Rickard M. D. , Mackinlay L. M. , Kane G. J. , Matossian R. M. , Smyth J. D.
Published: June 2009

Brood capsules were obtained from freshly collected cysts of equine and ovine strains ofEchinococcus granulosus. Protoscoleces were freed from brood capsules either by mechanical disruption or pepsin-HCl digestion. Preparations of protoscoleces studied included: mechanically released protoscoleces without further treatment, or incubated either in HCl pH 2·0 or in evaginating solution (containing Na taurocholate) for 24 h; pepsin-HCl released protoscoleces without further treatment or incubated in evaginating solution for 24 h or 7 days. Half of each preparation of ovine protoscoleces was fixed in absolute methanol.

All fresh preparations of protoscoleces lysed rapidly when incubated in normal human serum. Studies with a fluorescein isothiocyanate (FITC) labelled sheep anti-human C3antiserum revealed the presence of C3on the surface of lysing protoscoleces. Antibody could not be detected on the surface of any of the preparations of fresh or methanol-fixed protoscoleces using direct or indirect fluorescent antibody tests suggesting that the classical pathway of complement activation was not involved in the lytic process. Strong evidence for lysis by the alternate pathway of complement activation was the lysis of protoscoleces which had been treated with pepsin-HCl and lysis of protoscoleces in guinea-pig serum deficient in C4component of complement.
The fate of Fasciola hepatica metacercariae following challenge infection of immune rats
Authors: Rajasekariah G. R. , Howell M. J.
Published: June 2009

Groups of rats, infected 7 weeks previously with Fasciola hepatica, together with appropriate control groups, were challenged either orally or intraperitoneally with 30 metacercariae. The mean worm recovery from the previously infected, orally challenged rats was significantly lower than from their respective controls (2.2 ± 1.1 worms as opposed to 9.0 ± 2.6). There was no significant difference in mean worm recovery from the previously infected, intraperitoneally challenged rats and their respective controls (5.3 ± 3.2 worms as opposed to 6.2 ± 1.9). Livers of the orally challenged group appeared to be largely free from secondary damage but considerable damage was evident in rats which received an intraperitioneal challenge. This evidence supports the view that the gut acts as an important barrier to metacercariae of a challenge infection. In a further experiment, young flukes were recovered from the gut, abdominal cavity and liver of immune and control rats 9, 18, 27, 36 and 45 h after oral challenge. It was found that fewer flukes successfuly penetrated the guts of immune rats (3%) than those of uninfected controls (13%), again pointing to the gut as a barrier to metacercariae of a challenge infection. Protective mechanisms that may oprate at the level of the gut are discussed.
Monanema nilotican.sp. (Nematoda: Onchocercidae) a cardiopulmonary parasite of the Nile rat in Sudan
Authors: Elbihari Sabir , Hussein Hussein S. , Muller Ralph
Published: June 2009

Monanema nilotican.sp. (Nematoda: Onchocercidae) is described from the right auricle and pulmonary artery of the Nile rat,Arvicanthis niloticus testicularis(Sundevall, 1842), trapped in Khartoum-North, Sudan. It differs from other species principally in length of adults and microfilariae, in the shape of the buccal capsule and in the structure of the spicules of the males.
Modification of the pathogenicity of Schistosoma mattheei for sheep by passage of the parasite in hamsters
Authors: Taylor M. G. , James E. R. , Nelson G. S. , Bickle Q. , Dunne D. W. , Dobinson A. R. , Dargie J. D. , Berry C. I. , Hussein M. F.
Published: June 2009

Border Leicester X Suffolk sheep infected with a strain of S. mattheei maintained in hamsters do not develop the same pathological changes as Romney Marsh sheep infected with the same strain of parasite before hamster passage. To determine the cause of this reduced pathogenicity, five Romney Marsh sheep were each infected with 10 000 cercariae of the hamster-passaged parasite and five with 10 000 cercariae of a S. mattheei strain from Onderstepoort, South Africa, passaged exclusively through sheep.

Striking pathological and parasitological differences were found between the two strains. Infection with the “sheep” strain was lethal, whereas infection with the “hamster” strain produced little evidence of clinical disease. By 13 weeks post-infection the mean body weight of the sheep infected with the sheep strain had declined by 15% compared with both the uninfected controls and the sheep infected with the hamster strain, and the mean PCV was lowered to 20% in the sheep strain infected animals. Egg production began at seven weeks with the sheep strain, faecal counts rising to more than 300 e.p.g., whereas only two of the sheep infected with the hamster strain passed eggs in the faeces (at nine weeks) and the maximum egg count was 50 e.p.g. Twice as many adult worms of the sheep strain were recovered, and, although the number of eggs found in the tissues “per worm pair” was not significantly different, overall egg production was higher for the sheep strain; also more of the sheep strain eggs were deposited in the intestines. Similar parasite differences were seen in a supplementary study in mice and it seemed that “attenuation” of the parasite had occurred, presumably due to its maintenance in hamsters. Histopathological observations and faecal egg counts both indicated an inability of hamster strain eggs to penetrate the intestinal lumen; this was probably important in reducing the pathogenicity of the hamster strain.
Moniezia expansa: the interproglottidal glands and their Secretions
Authors: Gunn A. , Probert A. J.
Published: June 2009

Acerylcholinesterase (EC 3:1:1:7) and alkaline phoshatase (EC 3:1:3:1) were detected in secretions of Moniezia expansa maintained in vitro. Ultrastructural cytochemical studies demonstrated acetylcholinesterase activity on the surface of the microtriches at the base of the interproglittidal glands and in the gland lumen but not in the distal tegument or the gland cells. Alkaline phoshatase activity was demonstrated in the cytoplasm of the gland cells and especially in their protoplasmic connections with the distal tegument. Activity was also found in the distal tegument and the microtriches. It is suggested that the acetylcholinesterase secreted by M. expansa performs a metabolic role at the worms's surface.
The suppression of homologous immunity by soluble adult antigens of Nematospiroides dubius
Authors: Pritchard D. I. , Behnke J. M.
Published: June 2009

An established adult infection of Nematospiroides dubius was unaffected by the administration of immune lymphocytes and immune sera whereas an incoming larval infection was expelled. Past experiments have shown that the immune inoculum at least had the ability to recognize adult stages, leading to the hypothesis that adult stages secrete or excrete an immunomodulatory shield around themselves in the gastrointestinal tract. This hypothesis was given further credence by the demonstration that soluble antigens derived from adults abolished the generation of homologous immunity to this parasite. Modulation of immunity was reflected by increased fecundity, increased worm size, and increased survival time in the gut.
The life-cycle of Syphacia muris Yamaguti (Nematoda: Oxyuroidea) in the laboratory rat
Authors: Lewis J. W. , D'silva J.
Published: June 2009

The life-cycle of Syphacia muris is described in primary infections of Wistar rats. The life-cycle of S. muris is completed within seven days and no moults were discovered inside the egg. Instead each of two moults were observed outside the egg up to 24 and 40 hours after infection, and TEM studies suggest a third moult occurs up to 64 hours. The development and maturation of the larval stages are described for the first time using both light and electron microscopy. Differences in the size and growth of female S. muris compared with previously published figures could be due to differences in the strains of rats used.
Attempted vaccination of jirds (Meriones unguiculatus) againstBrugia pahangiwith radiation attenuated infective larvae
Authors: Chusattayanond W. , Denham D. A.
Published: June 2009

Jirds were vaccinated by three to five subcutaneous (SC) injections of infective larvae ofBrugia pahangiwhich had been irradiated at 25, 45 or 90 krads from a60Co source. They were challenged either SC or intraperitoneally. Vaccination with four doses of 50 larvae irradiated with 25 krads produced 49·3% resistance to IP challenge worms and 39·8% against SC challenge worms. Five doses of larvae irradiated with 45 krads produced 62% resistance to SC challenge. Three doses of larvae irradiated with 90 krads produced 74·9% resistance to SC challenge and five doses produced 76·2% resistance. The reasons why irradiated larvae produce resistance whereas normal larvae do not are discussed.
Optimization of culture conditions for the maintenance of Onchocerca gutturosa adult worms in vitro
Authors: Townson Simon , Connelly C. , Muller R.
Published: June 2009

A series of experiments examined the effects of various media, serum supplements, gas phases and the incorporation of mammalian cell feeder layers on the survival on Onchocerca gutturosa adult worms in vitro. The survival of male worms was poor in all media tested that were not supplemented with inactivated foetal calf serum (IFCS), with improved but variable survival in media supplemented with 10–30% IFCS. Using a cell-free system in an atmosphere of 5% CO2in air, good results were obtained in medium NCTC 135 + 10% IFCS (median survival time 39 days, range 25–41). Marginally better survival was obtained with the same medium in an atmosphere of 95% N2/5% CO2 (medium 45 days, range 25–56)and with a 1:1 mixture of media NCTC 135 and IMDM+10% IFCS(medium 38 days, range 38–51). Survival was enhanced in culture systems which incorporated bovine kindney (MDBK) cells, bovine trachea (EBTR) cells and monkey kidney (LLCMK2) cells. Exceptionally long survival was obtained using medium MEM+10% IFCS+LLCMK2 cells under a gas phase of 5% CO2in air, in which male worms survived from approximately 6 to over 7 months. Under similar conditions, female worms were also maintained for periods of up to 6 months and 5 out of 18 specimens released microfilariae into the culture system. The long-term culture described in this study will be useful for basic biochemical, chemotherapeutic and immunological studies in vitro.
Leukocyte accumulation in sparganosis: further characterization of an eosinophil chemotactic factor of the plerocercoid ofSpirometra erinacei
Authors: Horii Y. , Owhashi M. , Ishii A. , Fujita K.
Published: June 2009

An eosinophil chemotactic factor (ECF) was partially purified from plerocercoids ofSpirometra erinaceiby a combination of anion-exchange chromatography on DE52 and gel filtration on Sephacryl S–200. The molecular weight of ECF was estimated to be 25 000–45 000 by high-pressure liquid chromatography. The ECF was bound with concanavalin A-Sepharose. The ECF was sensitive to periodate oxidation and to heating (56°C, 30 min). On isoelectric focusing, eosinophil chemotactic activity was clearly revealed at pI 4·1. These results suggest that ECF ofS. erinaceiplerocercoid is an acidic glycoprotein. An intradermal injection of ECF induced eosinophil attractions in the normal guinea pig skin.
Observations on the suitability and importance of the domestic intermediate hosts of Echinococcus granulosus in Uttah Pradesh, India
Authors: Irshadullah M. , Nizami W. A. , Macpherson C. N. L.
Published: June 2009

The present study investigated the suitability and importance of buffaloes, camels, sheep, goats and pigs in maintaining the life-cycle of Echinococcus granulosus in Aligarh, India. A total of 565 (36%) of 1556 buffaloes, 20 (2%) of 1208 goats, 5 (1%) of 559 pigs, 6 (6%) of 109 sheep and two of three camels were found to harbour hydatid cysts. The frequency distribution of the hydatid cysts in each intermediate host species was over-dispersed and in buffaloes cyst fertility increased with increasing cyst size. Of 2171, 95 and four buffalo, goat, and camel cysts examined 327 (15%), two (2%) and three cysts respectively were fertile. No pig or sheep cysts were found to contain protoscoleces. The unfenced buffalo abattoir and the large number of dogs allowed access to the abattoir coupled to the number of buffaloes slaughtered in comparison to the other potential hosts, indicates that the buffalo is the most significant host for maintaining the life-cycle of the parasite in this area of India. Applicable control measures for the region are suggested.
Experimental Brugia pahangi and B. malayi infections of callitrichid primates
Authors: Denham D. A. , Suswillo R. R. , Hetherington C. M.
Published: June 2009

The callitrichid primates, Callithrix jacchus jacchus (the marmoset) and Saguinus labiatus (the tamarin) were inoculated with infective larvae of Brugia malayi and B. pahangi. Microfilaraemia at low levels developed in 3 out of 4 C. j. jacchus infected with B. malayi and living or dead adult worms found in all 4. Only one of 4 C. j. jacchus became microfilaraemic (mf + ve) when given B. pahangi and adults were found in two. Of 4 S. labiatus given B. pahangi one became very lightly mf + ve and adults were found in 3. It is concluded that these animals are not suitable hosts for chemotherapeutic experiments.
Attempts to control infection withOstertagia ostertagi(Trichostrongylidae) in grazing calves by adding mycelium of the nematode-trapping fungusArthrobotrys oligospora(Hyphomycetales) to cow pats
Authors: Grønvold J. , Henriksen S. A. , Nansen P. , Wolstrup J. , Thylin J.
Published: June 2009

Artificially prepared cow pats containingOstertagia ostertagieggs were deposited on two comparable grazing plots at weekly intervals during June and July 1987. Before deposition the cow pats, on the one plot, were inoculated with 0·250 g mycelial fragments per kg of faeces of the predacious fungusArthrobotrys oligospora. On the other control plot comparable numbers of non-inoculated cow pats were placed at the same time. Subsequently, in July, two equal groups of calves were turned out one on each plot to graze for two months after which they were necropsied. The number of infectiveO. ostertagilarvae in inoculated cow pats was reduced by 42% and herbage larval infectivity around them by 50–71% as compared with the corresponding parameters in control pats and surrounding herbage. After a grazing period of two months the calves on the plot with inoculated cow pats had acquired worm burdens that were on average 37% lower than those of calves on the control plot. The lower degree of parasitism in these calves was also reflected in lower egg counts, lower serum pepsinogen levels and higher body weight.
Immunocytochemical and ultrastructural studies on Dipetalonema viteae (Filarioidea)
Authors: Prüsse A. , Vollmer S. , Diesfeld H. J.
Published: June 2009

The antigenic properties of adult male and female of Dipetalonema viteae were studied by immunocytochemistry. Using antisera of the rodents Meriones unguiculatus and Mastomys natalensis infected with D. viteae, the binding of antibodies to sections of filariae embedded in Epon was assayed by the peroxidase-antiperoxidase (PAP) technique and by electron microscopy. The optimal staining intensity was obtained with an antiserum dilution of 1:5000. Control sera were obtained from sex and age matched uninfected animals.

Female D. viteae showed maximal antigen-antibody reactions within the uterus: in the inner uterus wall, in the “nutrient channels” between the maturing eggs and the differentiating microfilariae, on the eggshells, in the cuticula of microfilariae and in the spermatheca on the cell membrane of the mature spermatozoa. Male filariae showed binding of antibodies in the vesicula seminalis: in the nucleus and the nuclear membrane of primary spermatocytes and on maturing spermatids. Less pronounced antigen-antibody reactions in the cuticula, muscle and intestine were observed in both sexes.

The PAP-technique offers significant improvements in comparison with other techniques, e.g., immunofluorescence, used to detect antigens on filariae: the PAP-technique has an increased sensitivity with a concomitant reduction in nonspecific background and can be used for both light and electron microscopy; moreover, PAP-treated tissues can be stored indefinitely at room temperature.
Anisakis simplex (Rudolphi, 1809, det. Krabbe, 1878) (Nematoda: Ascaridoidea): Morphology and morphometry of larvae from euphausiids and fish, and a review of the life-history and ecology
Authors: Smith John W.
Published: June 2009

Larval Anisakis 7·7 to 23·6mm long from euphausiids, and larvae 18·0 to 21·9mm long from a teleost fish were morphologically and morphometrically alike and fitted the description by Beverley-Burton, Nyman & Pippy (1977) of A. simplex L3. Three small larvae, 4·2 to 5·9mm long, from euphausiids resembled L3 in anterior but not in posterior morphology; their tails lacked a mucron but, in two of them, cuticular separation at the posterior extremity revealed clearly the mucron characteristic of L3 within. These small larvae are identified as L2 of A. simplex in the process of moulting to L3. Japanese reports of typical L3 from 6·9 to 32·7 mm long in euphausiids, the occurrence in teleosts of typical L3 from only 8·8 to over 30 mm long, and the present observations strongly suggest that the moult from L2 to L3 occurs in euphausiids and begins when the larvae are about 4–6 mm long. Although it appears, therefore, that only one moult occurs in the egg of A. simplex (i.e. ensheathed L2 hatches), two moults there have been reported for various terrestrial and certain aquatic ascaridoids (i.e. ensheathed L3 hatches). The morphology and homology of the lips and associated sense organs of A simplex L3 are discussed relative to that of a ‘typical’ adult ascaridoid.

A main conclusion is that euphausiids in the North-East Atlantic and northern North Sea, and perhaps universally, are major intermediate hosts of A simplex. The status as hosts of squid and teleosts harbouring L3 is discussed. An extensive literature is reviewed in relation to the present observations.
Estimation of the numbers and infectivity of Nippostrongylus larvae
Authors: Keymer Anne , Martin Jean , Wainwright S. M.
Published: June 2009

Experimental investigations of the subcutaneous infection of third-stage larvae of Nippostrongylus brasiliensis reveal discrepancies between the actual and intended inoculum, and also variability between replicate inocula. Emphasis is thus placed on the importance of obtaining accurate estimates of the level of confidence associated with inoculum size. Under specified laboratory conditions, the retention of infectivity of N. brasiliensis larvae was found to be age-dependent, with a maximum of between 120 and 156 days. The motility of the larvae was not found to be indicative of their ability to complete migration and to become established in the small intestine of the host following subcutaneous inoculation.
Demonstration of collagenase activity in adult Strongyloides ratti (Nematoda: Strongyloididae) and its absence in the infective larvae
Authors: Wertheim Guta , Lustigman Sarah , Silberman Hermina , Shoshan Shmuel
Published: June 2009

Larvae and adults of Strongyloides ratti were examined for collagenolytic activity on 14C proline-labelled, native, guinea-pig skin collagen substrate. The activity was measured by determining either the amount of hydroxyproline released or the amount of radioactivity in the solubilized fraction of the collagen substrate. Bacterial collagenase was used for enzyme control and trypsin served as substrate control. No collagenolytic activity was found in living larvae, their extracts or metabolites. The collagenolytic activity of the metabolites of adult worms appeared weak, whereas that of the extracts of the adults was pronounced. It is suggested that collagenase is active in the adult females at the time of migration in the intestinal mucosa during oviposition.
Schistosomiasis in Swaziland—a comparative study of three irrigated estates
Authors: Logan James W. M.
Published: June 2009

The prevalence of schistosomiasis and the distribution of the snail intermediate hosts on three adjoining irrigated estates in the lowveld of Swaziland was investigated. The prevalence rates of Schistosoma haematobium and S. mansoni among estate employees was lower than anticipated and there was a marked difference between the prevalence rates of S. mansoni between estates. The reasons for this and the influence of water supplies and agricultural practice on the prevalence of schistosomiasis are discussed.
Shedding of gravid proglottids and destrobilation in experimental infections of foxes withMesocestoides leptothylacusLoos-Frank, 1980 (Cestoda)
Authors: Loos-Frank Brigitte
Published: June 2009

The implications of the confused taxonomy of the genusMesocestoidesand the misuse of the nameM. lineatusare described. In Southwest Germany rodents are intermediate hosts and red foxes are definitive hosts ofM. leptothylacus. The shedding patterns of experimentally infected foxes showed that destrobilation occurs frequently and that there are long periods during which no gravid proglottids are shed at all. Lengths of worms can be taken as a measure of a possible crowding effect only when worms with gravid segments are present, i.e. at the end of the prepatent period (11 to 13 days) or at the beginning of a shedding period.
Comparative study on polypeptide patterns of larvae ofTrichinellaisolates by two-dimensional electrophoresis
Authors: Garate T. , Rivas L.
Published: June 2009

The two-dimensional patterns (isoelectrofocusing-IEF/polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate-SDS) of S3fractions of muscle larvae of fourTrichinellaisolates were compared. The comparative study concerned six groups of polypeptides. It was observed that the Garkavi isolate ofTrichinella pseudospiraliswas clearly different from the other isolates, and it showed the simplest IEF/SDS polypeptide pattern. The C-76 isolate ofT. nelsonihad only four of the six groups, distinguishing it from the GM-1 isolate ofT. spiralisand the Boev isolate ofT. nativathat showed all the indicated groups.
Distribution of echinococcosis/hydatidosis in Italy
Authors: Lorenzini Rodolfo , Ruggieri Anna
Published: June 2009

Results of an epidemiological survey carried out in 88 slaughterhouses in Italy from 1979 to 1983 are reported. Data have been compared to national surveys previously conducted in 1955 and from 1968 to 1978. Analysis of various factors affecting the incidence of the disease in different areas of the country are reported.
Morphological and histochemical observations on the vitelline cells of developing and adult Paramphistomum cervi (Trematoda: Digenea)
Authors: Gupta B. C. , Parshad V. R. , Guraya S. S.
Published: June 2009

In 6 week-old Paramphistomum cervi, vitelline cells appear in the form of a few scattered cells, present below the body wall which divide mitotically to form vitelline follicles in 8 week-old worms. Small shell globules appear first in 10 week-old worms, the number and size of which continue to increase till the worm becomes adult. In the latter the vitelline follicles contain stem cells, immature, maturing and mature vitelline cells, which differ due to the distribution of shell globules as well as their histochemical nature. The shell globules are keratin in nature. The vitelline cells showed activities of various phosphatases but did not show the presence of any of the dehydrogenases studied.
Natural infection of Lymnaea truncatula by the liver fluke Fasciola hepatica in the Porma Basin, León, NW Spain
Authors: Manga-Gonzalez Y. , Gonzalez-Lanza C. , Otero-Merino C. B.
Published: June 2009

For two years fortnightly malacological samplings were carried out to collect Lymnaea truncatula (Mollusca; Basommatophora) at five points in the upper and middle Porma river basin, León, Spain. The highest numbers of snails were collected in September, May and November. Of the 5486 molluscs examined, 11·41% harboured F. hepatica (Trematoda; Digenea) with an average intensity of 20·14. In general, the values of both infection prevalence and intensity increased with the size of the snails. It was in October when the highest figures for each parameter mentioned above were detected (18·73% and 28·48, respectively). The chi-square test showed statistically significant differences in relation to the infection prevalence among the groups of molluscs established according to: their length; the months in which they were collected; the sampling localities; monthly average ambient temperature; precipitation during the collection. Similarly, statistically significant differences were detected in the intensity of the infection among the groups of molluscs previously established, except for that based on the values of precipitation. Generally, parasites were found in the same snail at different stages of development. It seems that most mollusc infections occur in February–March and at the end of summer–beginning of autumn periods. The highest rate of rediae with mature cercariae ready to be shed were detected between September and December. Metacercariae in the grass samples were also observed at the end of autumn. For this reason, this period could be considered as the most suitable for infection of the definitive hosts to take place.
The effects of age, sex and breed of chicken on susceptibility to Clinostomum complanatum (Digenea: Clinostomatidae)
Authors: Kalantan A. M. N. , Arfin M. , Nizami W. A.
Published: June 2009

Susceptibility and development of Clinostomum complanatum in the chicken (Gallus gallus domesticus) have been studied. The progenetic metacercariae of C. complanatum developed into ovigerous worms in 3 days in the buccal cavity of the chicken and survived only 6–7 days in this experimental host. The susceptibility is influenced by the age, sex and breed/type of the host. Both percent susceptibility and recovery of the worms decreased with increase in the age of the host. The egg laying chickens were found to be least susceptible to the infection. Mature male chickens were more susceptible than the females. The single comb white Leghorn (SCWL) was found to be susceptible while the local type (LT) and the cross breed (SCWL×LT) were not susceptible to C. complanatum. The possible reasons for these variations have been discussed.
Release and survival of Echinococcus eggs in different environments in Turkana, and their possible impact on the incidence of hydatidosis in man and livestock
Authors: Wachira T. M. , Macpherson C. N. L. , Gathuma J. M.
Published: June 2009

In Turkana, Kenya, a prevalence of hydatidosis of nearly 10% has been recorded among the pastoralists yet their livestock have a much lower prevalence of the disease. The present study investigated the release from dogs and subsequent survival of Echinococcus eggs in Turkana huts, water-holes and in the semi-arid environment. The results were compared with the survival of eggs of Taenia hydatigena and T. saginata. The study was repeated under the cooler and moister conditons found in Maasailand where livestock have a greater incidence of hydatid disease than in Turkana but where the incidence in man is ten times lower. The average number of Echinococcus eggs per proglottid is 823. Nine percent of these remain in proglottids 15 minutes after release from a dog and the released eggs lose their viability in less than two, 48 and 300 hours in the sun, huts and water in Turkana respectively; the major influencing factor being temperature. The greater survival of eggs in the houses, coupled with the fact that dogs congregate for most of the day in the small houses facilitating a close man:dog contact, provide ideal conditions for the trasmission of the parasite to man. The hostile environmental conditions and lack of contact between dogs and livestock contributes to the lower infection rate in livestock. Conversely in Maasailand, Echinococcus eggs survive in the environment for longer than three weeks and in addition, dogs are used for herding. This partly explains the higher infection rate among Maasai livestock but the low human infection rate remains arcane and requires further study. The rapid mortality of the majority of Echinococcus eggs in Turkana suggests that control measures aimed at dog control and a decreased man:dog contact should have a profound effect on the incidence of the disease in an area intrinsically unsuitable for the parasites' survival.
Survival of sheep and goat first stage protostrongylid larvae in experimental conditions: influence of humidity and temperature
Authors: Cabaret J. , Riseani S. Risye , Baeza E.
Published: June 2009

The survival of first-stage larvae of a laboratory strain of Muellerius capillaris and of a natural multispecific infection (Neostrongylus linearis, Cystocaulus nigrescens, Protostrongylus rufescens) was studied for 10 to 12 day periods. The survival was estimated either on larvae in faeces or kept in tap water. Temperature (−18°C to 37°C) and desiccation were the ecological factors investigated. M. capillaris was the most tolerant to these factors but showed better survival at 4°C (and at − 18°C on one occasion). N. linearis survived better at 25°C or − 18°C and C. nigrescens at 4°C and − 18°C. Humidification of faeces was unfavourable to the latter species. All the species could stand desiccation of faeces up to 67% of dry-matter for M. capillaris or 82% for other species. Larval survival estimated for L1 in tap water was different from that estimated for larvae in faeces. The variation in resistance to unfavourable temperatures or moisture conditions may account partly for the geographical distribution of the species