bluuebunny/crossref_metadata_2025 huggingface dataset:
Implementation of an Environmental Cleaning Protocol in Hospital Critical Areas Using a UV-C Disinfection Robot
Authors: Casini Beatrice , Tuvo Benedetta , Scarpaci Michela , Totaro Michele , Badalucco Federica , Briani Silvia , Luchini Grazia , Costa Anna Laura , Baggiani Angelo
Published: February 2023

Improving the cleaning and disinfection of high-touch surfaces is one of the core components of reducing healthcare-associated infections. The effectiveness of an enhanced protocol applying UV-C irradiation for terminal room disinfection between two successive patients was evaluated. Twenty high-touch surfaces in different critical areas were sampled according to ISO 14698-1, both immediately pre- and post-cleaning and disinfection standard operating protocol (SOP) and after UV-C disinfection (160 sampling sites in each condition, 480 in total). Dosimeters were applied at the sites to assess the dose emitted. A total of 64.3% (103/160) of the sampling sites tested after SOP were positive, whereas only 17.5% (28/160) were positive after UV-C. According to the national hygienic standards for health-care setting, 9.3% (15/160) resulted in being non-compliant after SOP and only 1.2% (2/160) were non-compliant after UV-C disinfection. Operation theaters was the setting that resulted in being less compliant with the standard limit (≤15 colony-forming unit/24 cm2) after SOP (12%, 14/120 sampling sites) and where the UV-C treatment showed the highest effectiveness (1.6%, 2/120). The addition of UV-C disinfection to the standard cleaning and disinfection procedure had effective results in reducing hygiene failures.
Exposure of the Gestating Mother to Sympathetic Stress Modifies the Cardiovascular Function of the Progeny in Male Rats
Authors: Piquer Beatriz , Olmos Diandra , Flores Andrea , Barra Rafael , Bahamondes Gabriela , Diaz-Araya Guillermo , Lara Hernan E.
Published: February 2023

Background: Sympathetic stress stimulates norepinephrine (NE) release from sympathetic nerves. During pregnancy, it modifies the fetal environment, increases NE to the fetus through the placental NE transporter, and affects adult physiological functions. Gestating rats were exposed to stress, and then the heart function and sensitivity to in vivo adrenergic stimulation were studied in male progeny. Methods: Pregnant Sprague–Dawley rats were exposed to cold stress (4 °C/3 h/day); rats’ male progeny were euthanized at 20 and 60 days old, and their hearts were used to determine the β-adrenergic receptor (βAR) (radioligand binding) and NE concentration. The in vivo arterial pressure response to isoproterenol (ISO, 1 mg/kg weight/day/10 days) was monitored in real time (microchip in the descending aorta). Results: Stressed male progeny presented no differences in ventricular weight, the cardiac NE was lower, and high corticosterone plasma levels were recorded at 20 and 60 days old. The relative abundance of β1 adrenergic receptors decreased by 36% and 45%, respectively (p < 0.01), determined by Western blot analysis without changes in β2 adrenergic receptors. A decrease in the ratio between β1/β2 receptors was found. Displacement of 3H-dihydroalprenolol (DHA) from a membrane fraction with propranolol (β antagonist), atenolol (β1 antagonist), or zinterol (β2 agonist) shows decreased affinity but no changes in the β-adrenergic receptor number. In vivo exposure to ISO to induce a β-adrenergic overload provoked death in 50% of stressed males by day 3 of ISO treatment. Conclusion: These data suggest permanent changes to the heart’s adrenergic response after rat progeny were stressed in the uterus.
Arsenic in Mining Areas: Environmental Contamination Routes
Authors: Faria Márcia , Hott Rodrigo , Santos Maicon , Santos Mayra , Andrade Thainá , Bomfeti Cleide , Rocha Bruno , Barbosa Fernando , Rodrigues Jairo
Published: February 2023

The emission and accumulation of toxic elements such as arsenic in various environmental compartments have become increasingly frequent primarily due to anthropogenic actions such as those observed in agricultural, industrial, and mining activities. An example of environmental arsenic contamination in Brazil exists in the city of Paracatu, MG, due to the operation of a gold mine. The aim of this work is to evaluate the routes and effects of arsenic contamination in environmental compartments (air, water, and soil) and environmental organisms (fish and vegetables) from mining regions as well as the trophic transfer of the element for a risk assessment of the population. In this study, high levels of arsenic were found in the waters of the Rico stream ranging from 4.05 µg/L during the summer season to 72.4 µg/L during the winter season. Moreover, the highest As concentration was 1.668 mg kg−1 in soil samples, which are influenced by seasonal variation and by proximity to the gold mine. Inorganic and organic arsenic species were found above the allowed limit in biological samples, indicating the transfer of arsenic found in the environment and demonstrating a great risk to the population exposed to this area. This study demonstrates the importance of environmental monitoring to diagnose contamination and encourage the search for new interventions and risk assessments for the population.
Static Postural Control during Single-Leg Stance in Endurance, Team and Combat Athletes from the Spanish National Sport Technification Program
Authors: Villarón-Casales Carlos , Aladro-Gonzalvo Arian Ramón , Gámez-Payá Javier , Pardo-Ibáñez Alberto , Domínguez-Navarro Fernando , Gallego Diana , Alarcón-Jimenez Jorge
Published: February 2023

In the context of pediatric physical exercise, the analysis of factors affecting postural control (PC) provides insight into the development of sport-specific motor skills. This study aims to evaluate the static PC during single-leg stance in endurance, team and combat athletes from the Spanish National Sport Technification Program. A total of 29 boys and 32 girls, aged 12 to 16 years old, were recruited. Centre of pressure (CoP) was measured on a force platform in standing position for 40 s under two sensorial and leg dominance conditions. Girls showed lower MVeloc (p < 0.001), MFreq (p > 0.001) and Sway (p < 0.001) values than boys in both sensorial conditions (open and closed eyes). The highest values in all PC variables were observed with eyes closed in both genders (p < 0.001). Sway values were lower in boys combat-athletes compared to endurance athletes in two sensorial conditions and with non-dominant leg (p < 0.05). Young athletes in their teens enrolled in a Sport Technification Program have shown differences in PC when comparing different visual conditions, sport disciplines and gender. This study opens a window to a better understanding of the determinants of PC during single-leg stance as a critical element in the sport specialization of young athletes.
Composition and Properties of Saliva in Patients with Osteoporosis Taking Antiresorptive Drugs
Authors: Sobczak-Jaskow Hanna , Kochańska Barbara , Drogoszewska Barbara
Published: February 2023

The aim of this study was to examine how the composition and properties of saliva change in people with osteoporosis who have received antiresorptive (AR) treatment, compared to patients with osteoporosis who have not yet received this treatment. Methods: The study population consisted of 38 patients with osteoporosis using AR drugs (Group I) and 16 patients with osteoporosis who had never used AR drugs (Group II). The control group consisted of 32 people without osteoporosis. Laboratory tests included determination of pH and concentrations of Ca, PO4, total protein, lactoferrin, lysozyme, sIgA, IgA, cortisol, neopterin, activity of amylase at rest, and stimulated saliva. The buffering capacity of stimulated saliva was also determined. Results: There were no statistically significant differences between the saliva of Group I and Group II. No statistically significant correlation was found between the amount of time using AR therapy (Group I) and the tested parameters of the saliva. Significant differences were found between Group I and the control group. The concentrations of PO4, lysozyme, and cortisol were higher, while concentrations of Ca ions, sIgA, and neopterin were lower, in comparison to the control group. The significant differences between Group II and the control group were smaller, and they concerned only the concentrations of lysozyme, cortisol, and neopterin. Conclusions: The saliva of people with osteoporosis subjected to AR therapy and those not subjected to AR therapy did not show statistically significant differences in terms of the examined parameters of the saliva. However, the saliva of patients with osteoporosis taking and not taking AR drugs was significantly different compared to the saliva of the control group.
The Brazilian System for Monitoring Workers and General Population Exposed to Asbestos: Development, Challenges, and Opportunities for Workers’ Health Surveillance
Authors: Buralli Rafael Junqueira , Pinheiro Regina Dal Castel , Susviela Laura Lima , Duracenko Sandra Renata Canale , De Capitani Eduardo Mello , Savaris Alexandre , Algranti Eduardo
Published: February 2023

The lack of safe levels of asbestos exposure and the long latency of asbestos-related disease (ARD) makes workers’ health surveillance challenging, especially in lower-income countries. This paper aims to present the recently developed Brazilian system for monitoring workers and general population exposed to asbestos (Datamianto), and to discuss the main challenges and opportunities for workers’ health surveillance. Methods: a descriptive study of the Datamianto development process, examining all the stages of system planning, development, improvement, validation, availability, and training of health services for its use, in addition to presenting the main challenges and opportunities for its implementation. Results: The system was developed by a group of software developers, workers’ health specialists, and practitioners, and it was recently incorporated by the Ministry of Health to be used for workers’ health surveillance. It can facilitate the monitoring of exposed individuals, epidemiological data analysis, promote cooperation between health services, and ensure periodical medical screening guaranteed to workers by labor legislation. Moreover, the system has a Business Intelligence (BI) platform to analyze epidemiologic data and produce near real-time reports. Conclusions: Datamianto can support and qualify the healthcare and surveillance of asbestos-exposed workers and ARD, promoting a better quality of life for workers and improving companies’ compliance with legislation. Even so, the system’s significance, applicability, and longevity will depend on the efforts aimed at its implementation and improvement.
Association of Religious Service Attendance and Neuropsychiatric Symptoms, Cognitive Function, and Sleep Disturbances in All-Cause Dementia
Authors: Britt Katherine Carroll , Richards Kathy C. , Acton Gayle , Hamilton Jill , Radhakrishnan Kavita
Published: February 2023

Commonly reported in dementia, neuropsychiatric symptoms (NPS), cognitive decline, and sleep disturbances indicate dementia progression. With the growing dementia burden, identifying protective factors that may slow dementia progression is increasingly essential. Religion and spirituality are associated with better mental and physical health, yet few studies have been reported in older adults with dementia. This study examines associations between religious service attendance and symptoms of dementia progression. Using data from the Health and Retirement Study in 2000, 2006, and 2008 and the sub-study, Aging, Demographics, and Memory Study in 2001–2003, 2006–2007, and 2008–2009, we examined the association of religious attendance with neuropsychiatric symptoms, cognitive function, and sleep disturbances among U.S. older adults aged 70 years and older with all-cause dementia (N = 72) using Spearman’s partial Rho correlation controlling for social interaction. Significant associations were identified for religious attendance and NPS (rs (97) = –0.124, 95% CI [–0.129, –0.119], p < 0.0005); cognitive function, rs (97) = –0.018, 95% CI [–0.023, –0.013], p < 0.001); and sleep disturbances, rs (97) = –0.275, 95% CI [–0.280, –0.271], p < 0.0005). Beyond adjusting for social interaction, increased religious attendance was associated with lower NPS, better cognitive function, and fewer sleep disturbances. Clinical trials and longitudinal studies with a larger sample size examining religion and spirituality factors with dementia progression are warranted.
Moving Morehouse School of Medicine Translation Tx Research through MDTTs—Multidisciplinary Translational Teams
Authors: Holliday Rhonda Conerly , Piper Kendra D. , Trimble Shawn X. , Dickinson-Copeland Carmen M. , Mitchell Ashley K. , Akintobi Tabia Henry , Bond Vincent C. , Floyd Virginia D.
Published: February 2023

Morehouse School of Medicine (SOM) works to achieve its vision of advancing health equity through conducting transformational, translation science (Tx). Tx describes our translational research continuum, symbolizing a method and scientific philosophy that intentionally promotes and supports convergence of interdisciplinary approaches and scientists to stimulate exponential advances for the health of diverse communities. Morehouse SOM actualizes Tx through multidisciplinary translational teams (MDTTs). We chronicle the identification of MDTTs by documenting formation, composition, functioning, successes, failures, and sustainability. Data and information were collected through key informant interviews, review of research documents, workshops, and community events. Our scan identified 16 teams that meet our Morehouse SOM definition of an MDTT. These team science workgroups cross basic science, clinical, and public health academic departments, and include community partners and student learners. We present four MDTTs, in various stages of progress, at Morehouse SOM and how they are advancing translational research.
Measurement and Coupling Coordination of High-Quality Development in Guangdong Province of China: A Spatiotemporal Analysis
Authors: Hu Jincan , Liang Junyi , Tian Litao , Wang Shaojian
Published: February 2023

Regional high-quality coordination plays a crucial role in promoting high-quality national development. Guangdong province is a trailblazer in China’s reform and opening-up policies and high-quality development. This study analyzes the high-quality development of the economic, social, and ecological environments from 2010 to 2019 in Guangdong with the entropy weight TOPSIS model. Meanwhile, the coupling coordination degree model is used to investigate the spatial-temporal pattern of the coupling and coordinated development of the three-dimensional system in 21 prefecture-level cities. The results show that the high-quality development index of Guangdong increased from 0.32 to 0.39, a 21.9% increase during 2010–2019. The Pearl River Delta had the highest value of the high-quality development index, whereas Western Guangdong had the lowest level in 2019. Guangzhou, Shenzhen, Zhuhai, and Dongguan are the core cities in the high-quality development of Guangdong, with the index decreasing from the Pearl River Delta estuary cities to the province’s edge. We also found that the coupling degree and coupling coordination of high-quality development of the three-dimensional system grew slowly during the study period. Half of the cities in Guangdong entered the stage of benign coupling. Except for Zhaoqing, all cities in the Pearl River Delta have a high coupling coordination degree of high-quality development of the three-dimensional system. This study provides valuable references for the high-quality, coordinated development of Guangdong province and some policy suggestions for other regions.
Changes in Bacterial Communities and Their Effects on Soil Carbon Storage in Spartina alterniflora Invasion Areas, Coastal Wetland Bare Flats, and Sueada salsa Areas
Authors: Liu Jiashuo , Duan Xiaoxiao , Li Guo , Cai Zhenjie , Wei Sijie , Song Qixuan , Zheng Zheng
Published: February 2023

Spartina alterniflora is considered an invasive species that has affected the biogeochemical circle of carbon in coastal wetlands around the world. Nevertheless, it is still unclear how S. alternation invasion affects the carbon storage capacity of coastal wetlands as carbon pools through bacterial changes. Herein, bacterial communities and soil carbon content in coastal wetland native areas and S. alterniflora invasion areas were detected. It was found that an S. alterniflora invasion brought more organic carbon and resulted in the increase in Proteobacteria in bare flats and Sueada salsa areas. When decomposition capacity was not sufficient, large amounts of organic carbon may be stored in specific chemical forms, such as monosaccharides, carboxylic acids, alcohols, etc. The results have also shown that soil bacterial communities were highly similar between the bare flat and S. alterniflora invasion area, which is extremely conducive to the rapid growth of S. alterniflora. However, an S. alterniflora invasion would decrease total carbon contents and inorganic carbon contents in the Sueada salsa area. This is not conducive to the stability of the soil carbon pool and soil health. These findings may complement, to some extent, the shortcomings of the interaction between S. alterniflora and bacterial communities, and their joint effect on soil carbon storage.
Analysis of the Degradation of OCPs Contaminated Soil by the BC/nZVI Combined with Indigenous Microorganisms
Authors: Li Qun , Zhang Lei , Wan Jinzhong , Fan Tingting , Deng Shaopo , Zhou Yan , He Yue
Published: February 2023

Organochlorine pesticides (OCPs) were typical persistent organic pollutants that posed great hazards and high risks in soil. In this study, a peanut shell biochar-loaded nano zero-valent iron (BC/nZVI) material was prepared in combination with soil indigenous microorganisms to enhance the degradation of α-hexachlorocyclohexane(α-HCH) and γ-hexachlorocyclohexane(γ-HCH) in water and soil. The effects of BC/nZVI on indigenous microorganisms in soil were investigated based on the changes in redox potential and dehydrogenase activity in the soil. The results showed as follows: (1) The specific surface area of peanut shell biochar loaded with nano-zero-valent iron was large, and the nano-zero-valent iron particles were evenly distributed on the peanut shell biochar; (2) peanut shell BC/nZVI had a good degradation effect on α-HCH and γ-HCH in water, with degradation rates of 64.18% for α-HCH and 91.87% for γ-HCH in 24 h; (3) peanut shell BC/nZVI also had a good degradation effect on α-HCH and γ-HCH in soil, and the degradation rates of α-HCH and γ-HCH in the 1% BC/nZVI reached 55.2% and 85.4%, second only to 1% zero-valent iron. The degradation rate was the fastest from 0 to 7 days, while the soil oxidation-reduction potential (ORP) increased sharply. (4) The addition of BC/nZVI to the soil resulted in a significant increase in dehydrogenase activity, which further promoted the degradation of HCHs; the amount of HCHs degradation was significantly negatively correlated with dehydrogenase activity. This study provides a remediation strategy for HCH-contaminated sites, reducing the human health risk of HCHs in the soil while helping to improve the soil and increase the activity of soil microorganisms.
Association between Metabolic Syndrome Status and Daily Physical Activity Measured by a Wearable Device in Japanese Office Workers
Authors: Yamaga Yukako , Svensson Thomas , Chung Ung-il , Svensson Akiko Kishi
Published: February 2023

(1) Background: This study examined the cross-sectional association between metabolic syndrome (MetS) status classified into three groups and daily physical activity (PA; step count and active minutes) using a wearable device in Japanese office workers. (2) Methods: This secondary analysis used data from 179 participants in the intervention group of a randomized controlled trial for 3 months. Individuals who had received an annual health check-up and had MetS or were at a high risk of MetS based on Japanese guidelines were asked to use a wearable device and answer questionnaires regarding their daily life for the entire study period. Multilevel mixed-effects logistic regression models adjusted for covariates associated with MetS and PA were used to estimate associations. A sensitivity analysis investigated the associations between MetS status and PA level according to the day of the week. (3) Results: Compared to those with no MetS, those with MetS were not significantly associated with PA, while those with pre-MetS were inversely associated with PA [step count Model 3: OR = 0.60; 95% CI: 0.36, 0.99; active minutes Model 3: OR = 0.62; 95% CI: 0.40, 0.96]. In the sensitivity analysis, day of the week was an effect modifier for both PA (p < 0.001). (4) Conclusions: Compared to those with no MetS, those with pre-MetS, but not MetS, showed significantly lower odds of reaching their daily recommended PA level. Our findings suggest that the day of the week could be a modifier for the association between MetS and PA. Further research with longer study periods and larger sample sizes are needed to confirm our results.
Influence of Humic Acids on the Removal of Arsenic and Antimony by Potassium Ferrate
Authors: Wang Ning , Li Wenwen , Wang Nannan , Li Man , Wang Hongbo
Published: February 2023

Although the removal ability of potassium ferrate (K2FeO4) on aqueous heavy metals has been confirmed by many researchers, little information focuses on the difference between the individual and simultaneous treatment of elements from the same family of the periodic table. In this project, two heavy metals, arsenic (As) and antimony (Sb) were chosen as the target pollutants to investigate the removal ability of K2FeO4 and the influence of humic acid (HA) in simulated water and spiked lake water samples. The results showed that the removal efficiencies of both pollutants gradually increased along the Fe/As or Sb mass ratios. The maximum removal rate of As(III) reached 99.5% at a pH of 5.6 and a Fe/As mass ratio of 4.6 when the initial As(III) concentration was 0.5 mg/L; while the maximum was 99.61% for Sb(III) at a pH of 4.5 and Fe/Sb of 22.6 when the initial Sb(III) concentration was 0.5 mg/L. It was found that HA inhibited the removal of individual As or Sb slightly and the removal efficiency of Sb was significantly higher than that of As with or without the addition of K2FeO4. For the co-existence system of As and Sb, the removal of As was improved sharply after the addition of K2FeO4, higher than Sb; while the latter was slightly better than that of As without K2FeO4, probably due to the stronger complexing ability of HA and Sb. X-ray energy dispersive spectroscopy (EDS), X-ray diffractometer (XRD), and X-ray photoelectron spectroscopy (XPS) were used to characterize the precipitated products to reveal the potential removal mechanisms based on the experimental results.
Grounding Wellness: Coloniality, Placeism, Land, and a Critique of “Social” Determinants of Indigenous Mental Health in the Canadian Context
Authors: Josewski Viviane , de Leeuw Sarah , Greenwood Margo
Published: February 2023

Authored by a small team of settler and Indigenous researchers, all of whom are deeply involved in scholarship and activism interrogating ongoing processes of coloniality in lands now known to many as Canada, this paper critically examines “social” and grounded determinants of Indigenous mental health and wellness. After placing ourselves on the grounds from which we write, we begin by providing an overview of the social determinants of health (SDOH), a conceptual framework with deep roots in colonial Canada. Though important in pushing against biomedical framings of Indigenous health and wellness, we argue that the SDOH framework nevertheless risks re-entrenching deeply colonial ways of thinking about and providing health services for Indigenous people: SDOH, we suggest, do not ultimately reckon with ecological, environmental, place-based, or geographic determinants of health in colonial states that continue to occupy stolen land. These theoretical interrogations of SDOH provide an entry point to, first, an overview of Indigenous ways of understanding mental wellness as tethered to ecology and physical geography, and second, a collection of narrative articulations from across British Columbia: these sets of knowledge offer clear and unequivocal evidence, in the form of Indigenous voices and perspectives, about the direct link between land, place, and mental wellness (or a lack thereof). We conclude with suggestions for future research, policy, and health practice actions that move beyond the current SDOH model of Indigenous health to account for and address the grounded, land-based, and ecologically self-determining nature of Indigenous mental health and wellness.
Experimental Study on Spread and Burning Characteristics of Continuous Spill Fire Leaked from a Point Source under Different Slopes
Authors: Sun Xiaoxiao , Huang Hong , Zhao Jinlong , Zhang Xiang , Song Guangheng
Published: February 2023

Liquid fuel is widely used in industry and transportation. Liquid fuel leakage usually results in some spill fire accidents. In this paper, the effect of slope on the spread and burning behaviors of continuous spill fire from a point discharge source was studied by experiments. The flame spread rate, burning rate, heat convection at the bottom surface, flame feedback radiation, and flame height were analyzed. The results show that the spread area has an increasing trend with the slope, and the length of the spread area increases obviously, while the width of spread area shows an opposite trend. Moreover, the burning rate and the flame height of the steady stage decreases significantly with the slope increase, which can be attributed to the increase of heat convection between the fuel layer and bottom for the larger slopes. Subsequently, a burning rate model for the steady stage is built considering fuel layer heat loss and validated by the current experimental data. This work can provide guidance for the thermal hazard analysis of liquid fuel spill fires from a point source.
Knowledge of Medical Imaging Professionals on Healthcare-Associated Infections: A Systematic Review and Meta-Analysis
Authors: Sukumar Suresh , Saha Shovan , Dkhar Winniecia , Panakkal Nitika C. , Nair Visakh Thrivikraman , Bommasamudram Tulasiram , Vaishali K , Nagaraja Ravishankar , Ravichandran Sneha , Kadavigere Rajagopal
Published: February 2023

Healthcare-associated infections (HCAIs) are a significant concern for both healthcare professionals and patients. With recent advances in imaging modalities, there is an increase in patients visiting the radiology department for diagnosis and therapeutic examination. The equipment used for the investigator is contaminated, which may result in HCAIs to the patients and healthcare professionals. Medical imaging professionals (MIPs) should have adequate knowledge to overcome the spread of infection in the radiology department. This systematic review aimed to examine the literature on the knowledge and precaution standard of MIPs on HCIAs. This study was performed with a relative keyword using PRISMA guidelines. The articles were retrieved from 2000 to 2022 using Scopus, PubMed, and ProQuest databases. The NICE public health guidance manual was used to assess the quality of the full-length article. The search yielded 262 articles, of which Scopus published 13 articles, PubMed published 179 articles, and ProQuest published 55 articles. In the present review, out of 262 articles, only 5 fulfilled the criteria that reported MIPs’ knowledge of Jordan, Egypt, Sri Lanka, France, and Malawi populations. The present review reported that MIPs have moderate knowledge and precautionary standards regarding HCIAs in the radiology department. However, due to the limited studies published in the literature, the current review limits the application of the outcome in the vast MIPs population. This review recommended further studies to be conducted among the MIPs worldwide to know the actual knowledge and precaution standards regarding HCIAs.
Exploring the Dynamics between Specialization and De-Specialization of Welfare Experiences: A Qualitative Study of the Special Families under the One-Child Policy in China
Authors: Cheng Sheng-Li , Yu Miao , Liu Shu-Shan , Li Yun
Published: February 2023

The one-child policy, i.e., of having only one child per couple, was adopted as the essential family policy in China from 1979, and since the beginning of the 21st century, it has given rise to problems of special families under the one-child policy caused by the death or disability of only children. The existing research focused on the issue of special families from a macro-social level and analyzed the welfare demands and welfare policies of those families, whereas less research has been concerned with the families’ individual experiences and interpretations. This study adopted a qualitative research method and conducted in-depth interviews with 33 participants to analyze the welfare experiences of special families in Jinan city, Shandong Province. The findings of the study were based on generalized analyses of the interviews, including the “specialization” dimension of welfare experiences with identity-oriented, targeted, and comprehensive characteristics, the “de-specialization” dimension of welfare experiences with identity-denied, excluded, and hidden characteristics. The dynamics between the two dimensions among different special families, different family members, and different periods in the families’ lives were also examined. We present a discussion of the study’s findings and their implications, categorized into the theoretical and practical domains.
A Systematic Review of Child Health, Developmental and Educational Outcomes Associated with High Mobility in Indigenous Children from Australia, Canada and New Zealand
Authors: Nikolof Arwen , Brown Stephanie J. , Clark Yvonne , Glover Karen , Gartland Deirdre
Published: February 2023

Indigenous families tend to move house more often, especially families with young children. However, little is known about the impact of high mobility on children’s well-being and development. The aim of this systematic review was to examine the relationship between residential mobility and children’s health, developmental, and educational outcomes for Australian, Canadian, and New Zealand Indigenous children (0–12 years). Four databases were investigated with pre-determined inclusion and exclusion criteria. The search identified 243 articles after independent screening by two authors. Eight studies assessing four child health outcomes were included, six quantitative and two qualitative. Child health outcomes were classified into four broad categories—physical health, social and emotional behavior, learning and development, and developmental risk. The review identified limited evidence; possible links were identified between high mobility and emotional and behavioral difficulties for younger children. One study identified evidence of a linear relationship between the number of houses a child has lived in since birth and developmental risk. Further research is needed to fully understand the impact of high residential mobility for Indigenous children at different developmental stages. Prioritizing the involvement, collaboration, and empowerment of Indigenous communities and leadership is critical for future research.
Physical, Psychiatric, and Social Comorbidities of Individuals with Schizophrenia Living in the Community in Japan
Authors: Matsunaga Masaaki , Li Yuanying , He Yupeng , Kishi Taro , Tanihara Shinichi , Iwata Nakao , Tabuchi Takahiro , Ota Atsuhiko
Published: February 2023

The physical, psychiatric, and social comorbidities interfere with the everyday activities of community-dwelling individuals with schizophrenia and increase the risk of their readmission. However, these comorbidities have not been investigated comprehensively in Japan. We conducted a self-reported internet survey in February 2022 to identify individuals aged 20–75 years with and without schizophrenia using a prevalence case-control study. The survey compared physical comorbidities such as being overweight, hypertension, and diabetes; psychiatric comorbidities such as depressive symptoms and sleep disturbances; social comorbidities such as employment status, household income, and social support between participants with and without schizophrenia. A total of 223 participants with schizophrenia and 1776 participants without schizophrenia were identified. Participants with schizophrenia were more likely to be overweight and had a higher prevalence of hypertension, diabetes, and dyslipidemia than participants without schizophrenia. Additionally, depressive symptoms, unemployment, and non-regular employment were more prevalent in participants with schizophrenia than those without schizophrenia. These results highlight the necessity of comprehensive support and interventions addressing physical, psychiatric, and social comorbidities in individuals with schizophrenia in the community. In conclusion, effective interventions for managing comorbidities in individuals with schizophrenia are necessary to enable them to continue to live in the community.
Oral Administration of Lipopolysaccharide Enhances Insulin Signaling-Related Factors in the KK/Ay Mouse Model of Type 2 Diabetes Mellitus
Authors: Yamamoto Kazushi , Yamashita Masashi , Oda Masataka , Tjendana Tjhin Vindy , Inagawa Hiroyuki , Soma Gen-Ichiro
Published: February 2023

Lipopolysaccharide (LPS), an endotoxin, induces systemic inflammation by injection and is thought to be a causative agent of chronic inflammatory diseases, including type 2 diabetes mellitus (T2DM). However, our previous studies found that oral LPS administration does not exacerbate T2DM conditions in KK/Ay mice, which is the opposite of the response from LPS injection. Therefore, this study aims to confirm that oral LPS administration does not aggravate T2DM and to investigate the possible mechanisms. In this study, KK/Ay mice with T2DM were orally administered LPS (1 mg/kg BW/day) for 8 weeks, and blood glucose parameters before and after oral administration were compared. Abnormal glucose tolerance, insulin resistance progression, and progression of T2DM symptoms were suppressed by oral LPS administration. Furthermore, the expressions of factors involved in insulin signaling, such as insulin receptor, insulin receptor substrate 1, thymoma viral proto-oncogene, and glucose transporter type 4, were upregulated in the adipose tissues of KK/Ay mice, where this effect was observed. For the first time, oral LPS administration induces the expression of adiponectin in adipose tissues, which is involved in the increased expression of these molecules. Briefly, oral LPS administration may prevent T2DM by inducing an increase in the expressions of insulin signaling-related factors based on adiponectin production in adipose tissues.
Structurally Different Yet Functionally Similar: Aptamers Specific for the Ebola Virus Soluble Glycoprotein and GP1,2 and Their Application in Electrochemical Sensing
Authors: Banerjee Soma , Hemmat Mahsa Askary , Shubham Shambhavi , Gosai Agnivo , Devarakonda Sivaranjani , Jiang Nianyu , Geekiyanage Charith , Dillard Jacob A. , Maury Wendy , Shrotriya Pranav , Lamm Monica H. , Nilsen-Hamilton Marit
Published: February 2023

The Ebola virus glycoprotein (GP) gene templates several mRNAs that produce either the virion-associated transmembrane protein or one of two secreted glycoproteins. Soluble glycoprotein (sGP) is the predominant product. GP1 and sGP share an amino terminal sequence of 295 amino acids but differ in quaternary structure, with GP1 being a heterohexamer with GP2 and sGP a homodimer. Two structurally different DNA aptamers were selected against sGP that also bound GP1,2. These DNA aptamers were compared with a 2′FY-RNA aptamer for their interactions with the Ebola GP gene products. The three aptamers have almost identical binding isotherms for sGP and GP1,2 in solution and on the virion. They demonstrated high affinity and selectivity for sGP and GP1,2. Furthermore, one aptamer, used as a sensing element in an electrochemical format, detected GP1,2 on pseudotyped virions and sGP with high sensitivity in the presence of serum, including from an Ebola-virus-infected monkey. Our results suggest that the aptamers interact with sGP across the interface between the monomers, which is different from the sites on the protein bound by most antibodies. The remarkable similarity in functional features of three structurally distinct aptamers suggests that aptamers, like antibodies, have preferred binding sites on proteins.
Advanced Situation with Recombinant Toxins: Diversity, Production and Application Purposes
Authors: Efremenko Elena , Aslanli Aysel , Lyagin Ilya
Published: February 2023

Today, the production and use of various samples of recombinant protein/polypeptide toxins is known and is actively developing. This review presents state-of-the-art in research and development of such toxins and their mechanisms of action and useful properties that have allowed them to be implemented into practice to treat various medical conditions (including oncology and chronic inflammation applications) and diseases, as well as to identify novel compounds and to detoxify them by diverse approaches (including enzyme antidotes). Special attention is given to the problems and possibilities of the toxicity control of the obtained recombinant proteins. The recombinant prions are discussed in the frame of their possible detoxification by enzymes. The review discusses the feasibility of obtaining recombinant variants of toxins in the form of protein molecules modified with fluorescent proteins, affine sequences and genetic mutations, allowing us to investigate the mechanisms of toxins’ bindings to their natural receptors.
Stem Cells in Kidney Ischemia: From Inflammation and Fibrosis to Renal Tissue Regeneration
Authors: Cianci Rosario , Simeoni Mariadelina , Cianci Eleonora , De Marco Oriana , Pisani Antonio , Ferri Claudio , Gigante Antonietta
Published: February 2023

Ischemic nephropathy consists of progressive renal function loss due to renal hypoxia, inflammation, microvascular rarefaction, and fibrosis. We provide a literature review focused on kidney hypoperfusion-dependent inflammation and its influence on renal tissue’s ability to self-regenerate. Moreover, an overview of the advances in regenerative therapy with mesenchymal stem cell (MSC) infusion is provided. Based on our search, we can point out the following conclusions: 1. endovascular reperfusion is the gold-standard therapy for RAS, but its success mostly depends on treatment timeliness and a preserved downstream vascular bed; 2. anti-RAAS drugs, SGLT2 inhibitors, and/or anti-endothelin agents are especially recommended for patients with renal ischemia who are not eligible for endovascular reperfusion for slowing renal damage progression; 3. TGF-β, MCP-1, VEGF, and NGAL assays, along with BOLD MRI, should be extended in clinical practice and applied to a pre- and post-revascularization protocols; 4. MSC infusion appears effective in renal regeneration and could represent a revolutionary treatment for patients with fibrotic evolution of renal ischemia.
Phloroglucinol Inhibits Oxidative-Stress-Induced Cytotoxicity in C2C12 Murine Myoblasts through Nrf-2-Mediated Activation of HO-1
Authors: Park Cheol , Cha Hee-Jae , Hwangbo Hyun , Ji Seon Yeong , Kim Da Hye , Kim Min Yeong , Bang EunJin , Hong Su Hyun , Kim Sung Ok , Jeong Soon-Jeong , Lee Hyesook , Moon Sung-Kwon , Shim Jung-Hyun , Kim Gi-Young , Cho Suengmok , Choi Yung Hyun
Published: February 2023

Phloroglucinol is a class of polyphenolic compounds containing aromatic phenyl rings and is known to have various pharmacological activities. Recently, we reported that this compound isolated from Ecklonia cava, a brown alga belonging to the family Laminariaceae, has potent antioxidant activity in human dermal keratinocytes. In this study, we evaluated whether phloroglucinol could protect against hydrogen peroxide (H2O2)-induced oxidative damage in murine-derived C2C12 myoblasts. Our results revealed that phloroglucinol suppressed H2O2-induced cytotoxicity and DNA damage while blocking the production of reactive oxygen species. We also found that phloroglucinol protected cells from the induction of apoptosis associated with mitochondrial impairment caused by H2O2 treatment. Furthermore, phloroglucinol enhanced the phosphorylation of nuclear factor-erythroid-2 related factor 2 (Nrf2) as well as the expression and activity of heme oxygenase-1 (HO-1). However, such anti-apoptotic and cytoprotective effects of phloroglucinol were greatly abolished by the HO-1 inhibitor, suggesting that phloroglucinol could increase the Nrf2-mediated activity of HO-1 to protect C2C12 myoblasts from oxidative stress. Taken together, our results indicate that phloroglucinol has a strong antioxidant activity as an Nrf2 activator and may have therapeutic benefits for oxidative-stress-mediated muscle disease.
Aluminum and Fluoride Stresses Altered Organic Acid and Secondary Metabolism in Tea (Camellia sinensis) Plants: Influences on Plant Tolerance, Tea Quality and Safety
Authors: Peng Anqi , Yu Keke , Yu Shuwei , Li Yingying , Zuo Hao , Li Ping , Li Juan , Huang Jianan , Liu Zhonghua , Zhao Jian
Published: February 2023

Tea plants have adapted to grow in tropical acidic soils containing high concentrations of aluminum (Al) and fluoride (F) (as Al/F hyperaccumulators) and use secret organic acids (OAs) to acidify the rhizosphere for acquiring phosphorous and element nutrients. The self-enhanced rhizosphere acidification under Al/F stress and acid rain also render tea plants prone to accumulate more heavy metals and F, which raises significant food safety and health concerns. However, the mechanism behind this is not fully understood. Here, we report that tea plants responded to Al and F stresses by synthesizing and secreting OAs and altering profiles of amino acids, catechins, and caffeine in their roots. These organic compounds could form tea-plant mechanisms to tolerate lower pH and higher Al and F concentrations. Furthermore, high concentrations of Al and F stresses negatively affected the accumulation of tea secondary metabolites in young leaves, and thereby tea nutrient value. The young leaves of tea seedlings under Al and F stresses also tended to increase Al and F accumulation in young leaves but lower essential tea secondary metabolites, which challenged tea quality and safety. Comparisons of transcriptome data combined with metabolite profiling revealed that the corresponding metabolic gene expression supported and explained the metabolism changes in tea roots and young leaves via stresses from high concentrations of Al and F. The study provides new insight into Al- and F-stressed tea plants with regard to responsive metabolism changes and tolerance strategy establishment in tea plants and the impacts of Al/F stresses on metabolite compositions in young leaves used for making teas, which could influence tea nutritional value and food safety.
Vitamin D and Vitamin D-Binding Protein in Health and Disease
Authors: Delrue Charlotte , Speeckaert Marijn M.
Published: February 2023

Vitamin D is a fat-soluble secosteroid that exists in two forms: vitamin D2 and vitamin D3 [...]
Genome-Wide Analysis of the LATERAL ORGAN BOUNDARIES Domain (LBD) Members in Alfalfa and the Involvement of MsLBD48 in Nitrogen Assimilation
Authors: Jiang Xu , Cui Huiting , Wang Zhen , Kang Junmei , Yang Qingchuan , Guo Changhong
Published: February 2023

The LATERAL ORGAN BOUNDARIES DOMAIN (LBD) proteins, a transcription factor family specific to the land plants, have been implicated in multiple biological processes including organ development, pathogen response and the uptake of inorganic nitrogen. The study focused on LBDs in legume forage Alfalfa. The genome-wide analysis revealed that in Alfalfa 178 loci across 31 allelic chromosomes encoded 48 unique LBDs (MsLBDs), and the genome of its diploid progenitor M. sativa spp. Caerulea encoded 46 LBDs. Synteny analysis indicated that the expansion of AlfalfaLBDs was attributed to the whole genome duplication event. The MsLBDs were divided into two major phylogenetic classes, and the LOB domain of the Class I members was highly conserved relative to that of the Class II. The transcriptomic data demonstrated that 87.5% of MsLBDs were expressed in at least one of the six test tissues, and Class II members were preferentially expressed in nodules. Moreover, the expression of Class II LBDs in roots was upregulated by the treatment of inorganic nitrogen such as KNO3 and NH4Cl (0.3 mM). The overexpression of MsLBD48, a Class II member, in Arabidopsis resulted in growth retardance with significantly declined biomass compared with the non-transgenic plants, and the transcription level of the genes involved in nitrogen uptake or assimilation, including NRT1.1, NRT2.1, NIA1 and NIA2 was repressed. Therefore, the LBDs in Alfalfa are highly conserved with their orthologs in embryophytes. Our observations that ectopic expression of MsLBD48 inhibited Arabidopsis growth by repressing nitrogen adaption suggest the negative role of the transcription factor in plant uptake of inorganic nitrogen. The findings imply the potential application of MsLBD48 in Alfalfa yield improvement via gene editing.
Extracellular Vesicles (EVs) as Crucial Mediators of Cell-Cell Interaction in Asthma
Authors: Tinè Mariaenrica , Padrin Ylenia , Bonato Matteo , Semenzato Umberto , Bazzan Erica , Conti Maria , Saetta Marina , Turato Graziella , Baraldo Simonetta
Published: February 2023

Asthma is the most common chronic respiratory disorder worldwide and accounts for a huge health and economic burden. Its incidence is rapidly increasing but, in parallel, novel personalized approaches have emerged. Indeed, the improved knowledge of cells and molecules mediating asthma pathogenesis has led to the development of targeted therapies that significantly increased our ability to treat asthma patients, especially in severe stages of disease. In such complex scenarios, extracellular vesicles (EVs i.e., anucleated particles transporting nucleic acids, cytokines, and lipids) have gained the spotlight, being considered key sensors and mediators of the mechanisms controlling cell-to-cell interplay. We will herein first revise the existing evidence, mainly by mechanistic studies in vitro and in animal models, that EV content and release is strongly influenced by the specific triggers of asthma. Current studies indicate that EVs are released by potentially all cell subtypes in the asthmatic airways, particularly by bronchial epithelial cells (with different cargoes in the apical and basolateral side) and inflammatory cells. Such studies largely suggest a pro-inflammatory and pro-remodelling role of EVs, whereas a minority of reports indicate protective effects, particularly by mesenchymal cells. The co-existence of several confounding factors—including technical pitfalls and host and environmental confounders—is still a major challenge in human studies. Technical standardization in isolating EVs from different body fluids and careful selection of patients will provide the basis for obtaining reliable results and extend their application as effective biomarkers in asthma.