bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiparametric MRI may Help to Identify Patients With Prostate Cancer in a Contemporary Cohort of Patients With Clinical Bladder Outlet Obstruction Scheduled for Holmium Laser Enucleation of the Prostate (HoLEP)
Authors: Wenzel Mike , Welte Maria N. , Grossmann Lina , Preisser Felix , Theissen Lena H. , Humke Clara , Deuker Marina , Bernatz Simon , Gild Philipp , Ahyai Sascha , Karakiewicz Pierre I. , Bodelle Boris , Kluth Luis A. , Chun Felix K. H. , Mandel Philipp , Becker Andreas
Published: February 2021

Objective: To investigate the value of standard [digital rectal examination (DRE), PSA] and advanced (mpMRI, prostate biopsy) clinical evaluation for prostate cancer (PCa) detection in contemporary patients with clinical bladder outlet obstruction (BOO) scheduled for Holmium laser enucleation of the prostate (HoLEP).

Material and Methods: We retrospectively analyzed 397 patients, who were referred to our tertiary care laser center for HoLEP due to BOO between 11/2017 and 07/2020. Of those, 83 (20.7%) underwent further advanced clinical PCa evaluation with mpMRI and/or prostate biopsy due to elevated PSA and/or lowered PSA ratio and/or suspicious DRE. Logistic regression and binary regression tree models were applied to identify PCa in BOO patients.

Results: An mpMRI was conducted in 56 (66%) of 83 patients and revealed PIRADS 4/5 lesions in 14 (25%) patients. Subsequently, a combined systematic randomized and MRI-fusion biopsy was performed in 19 (23%) patients and revealed in PCa detection in four patients (5%). A randomized prostate biopsy was performed in 31 (37%) patients and revealed in PCa detection in three patients (4%). All seven patients (9%) with PCa detection underwent radical prostatectomy with 29% exhibiting non-organ confined disease. Incidental PCa after HoLEP (n = 76) was found in nine patients (12%) with advanced clinical PCa evaluation preoperatively. In univariable logistic regression analyses, PSA, fPSA ratio, and PSA density failed to identify patients with PCa detection. Conversely, patients with a lower International Prostate Symptom Score (IPSS) and PIRADs 4/5 lesion in mpMRI were at higher risk for PCa detection. In multivariable adjusted analyses, PIRADS 4/5 lesions were confirmed as an independent risk factor (OR 9.91, p = 0.04), while IPSS did not reach significance (p = 0.052).

Conclusion: In advanced clinical PCa evaluation mpMRI should be considered in patients with elevated total PSA or low fPSA ratio scheduled for BOO treatment with HoLEP. Patients with low IPSS or PIRADS 4/5 lesions in mpMRI are at highest risk for PCa detection. In patients with a history of two or more sets of negative prostate biopsies, advanced clinical PCa evaluation might be omitted.
Differential Effects of Dietary Oregano Essential Oil on the Inflammation Related Gene Expression in Peripheral Blood Mononuclear Cells From Outdoor and Indoor Reared Pigs
Authors: Cappelli Katia , Sabino Marcella , Trabalza-Marinucci Massimo , Acuti Gabriele , Capomaccio Stefano , Menghini Luigi , Verini-Supplizi Andrea
Published: February 2021

Intensive farming systems represent a stressful environment for pigs and negatively influence neuroendocrine functions, behavior, and performance. Outdoor farming is an alternative option, which is thought to imply several beneficial effects for the animal. Dietary essential oils are known to be an innovative strategy to improve pig health and performance, and oregano essential oil (ORE) possesses beneficial effects due to its antimicrobial, anti-fungal, and antioxidant properties. We tested the effect of dietary ORE on peripheral blood mononuclear cells (PBMCs) in 36 growing pigs, either reared under indoor or outdoor conditions. Quantitative real-time PCR (RT-qPCR) assay was used to evaluate the effect of diet (control vs. ORE) and the time of sampling (T1−120 days vs. T2−190 days) on the expression of inflammatory and immune-related genes (TNF, IL1β, IL8, IL18, IL10, IL1RN, STAT3, HSP90, ICAM-1, and NFKB1). Under outdoor condition, the majority of transcripts were upregulated (p < 0.05), assuming a general inflammatory status (TNF, HSP90, NFKB1, IL1β, and STAT3). However, an interaction between diet and the farming system was observed: HSP90, NFKB1, and STAT3 were downregulated (p < 0.05) in the outdoor reared pigs when fed the ORE diet. Our study showed that bioactive compounds of ORE exert their activity, especially when the animals are exposed to stressful stimuli. Dietary ORE can be an acceptable strategy to help pigs tolerate the stress related to the harsh, outdoor, rearing conditions.
Quantification of Global DNA Methylation in Canine Mammary Gland Tumors via Immunostaining of 5-Methylcytosine: Histopathological and Clinical Correlations
Authors: Biondi Luiz Roberto , Tedardi Marcello Vannucci , Gentile Luciana Boffoni , Chamas Patricia Pereira Costa , Dagli Maria Lucia Zaidan
Published: February 2021

Mammary tumors are the most prevalent neoplasms in non-neutered female dogs, with genetic and epigenetic alterations contributing to canine mammary carcinogenesis. This study quantified global DNA methylation in 5-methylcytosine (5mC)-immunostained canine mammary tumor samples and established histopathological and clinical correlations. A total of 91 formalin-fixed paraffin-embedded mammary tumor samples from female dogs were retrospectively selected and subjected to immunohistochemistry using an anti-5mC mouse monoclonal antibody. We evaluated 5mC+ stained nuclei of neoplastic epithelial cells in canine mammary glands to obtain semiquantitative histoscores based on staining intensity. Survival rates were estimated based on owners' or veterinary records. Histological samples comprised 28 and 63 benign and malignant canine mammary gland tumors, respectively. Results revealed significant differences between global DNA methylation patterns when mammary samples were categorized as benign or malignant (p = 0.024), with hypomethylated patterns more prevalent in malignant tumors and those with higher relapse behavior (p = 0.011). Of note, large diameter (>5 cm) tumors revealed a lower methylation pattern (p = 0.028). Additionally, we found non-statistically significant differences when tumors were grouped by histopathological characteristics, clinical parameters, or survival. These findings propose global DNA methylation assessment as a promising tool for detecting canine mammary tumors with relapse propensity.
The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O
Authors: Xiao Yan , Zhang Suling , Yan He , Geng Xiaolin , Wang Yanwei , Xu Xin , Wang Mengyue , Zhang Haohao , Huang Baicheng , Pang Wenqiang , Yang Ming , Tian Kegong
Published: February 2021

Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)–fused capsid proteins of FMDV serotype O by single plasmid in Escherichia coli was achieved with an optimal tandem permutation (VP0–VP3–VP1), showing a protein yield close to 1:1:1. After SUMO removal at a low level of protease activity (5 units), the assembled FMDV virus-like particles (VLPs) could expose multiple epitopes and have a size similar to the naive FMDV. Immunization of pigs with the FMDV VLPs could induce FMDV-specific humoral and cellular immune responses effectively, in a dose-dependent manner. These data suggested that the stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, which laid a foundation for the further development of the FMDV subunit vaccine.
A Simple Dynamic Characterization Method for Thin Stacked Dielectric Elastomer Actuators by Suspending a Weight in Air and Electrical Excitation
Authors: Takagi Kentaro , Kitazaki Yuya , Kondo Kota
Published: February 2021

This paper proposes a simple but effective method for characterizing dielectric elastomer actuators (DEAs), especially for thin stacked DEAs, which are promising for haptic devices but which measure the dynamic elastic modulus with great difficulty. The difficulty of the measurement of such a thin stacked DEA arises from the friction and local deformation of the surface between the DEA and a contact, as shown in this paper. In the proposed method, a DEA is vertically suspended and a weight is attached to it. The proposed method requires no contact with the surface of a DEA and uses only a weighting mass. Experimental results demonstrated the proposed method can estimate almost essential constants, such as the dynamic elastic modulus (Young’s modulus and damping time constant), the electrical constants (permittivity and resistivity), and the coefficient of electromechanical coupling, through the forced vibration induced by voltage actuation.
Reducing N Fertilization without Yield Penalties in Maize with a Commercially Available Seed Dressing
Authors: Maris Stefania Codruta , Capra Federico , Ardenti Federico , Chiodini Marcello E. , Boselli Roberta , Taskin Eren , Puglisi Edoardo , Bertora Chiara , Poggianella Lorenzo , Amaducci Stefano , Tabaglio Vincenzo , Fiorini Andrea
Published: February 2021

Introducing smart and sustainable tools for climate change adaptation and mitigation is a major need to support agriculture’s productivity potential. We assessed the effects of the processed gypsum seed dressing SOP® COCUS MAIZE+ (SCM), combined with a gradient of N fertilization rates (i.e., 0%, 70% equal to 160 kg N ha−1, and 100% equal to 230 kg N ha−1) in maize (Zea mays L.), on: (i) grain yield, (ii) root length density (RLD) and diameter class length (DCL), (iii) biodiversity of soil bacteria and fungi, and (iv) Greenhouse Gases (GHGs, i.e., N2O, CO2, and CH4) emission. Grain yield increased with SCM by 1 Mg ha−1 (+8%). The same occurred for overall RLD (+12%) and DCL of very fine, fine, and medium root classes. At anthesis, soil microbial biodiversity was not affected by treatments, suggesting earlier plant-rhizosphere interactions. Soil GHGs showed that (i) the main driver of N losses as N2O is the N-fertilization level, and (ii) decreasing N-fertilization in maize from 100% to 70% decreased N2O emissions by 509 mg N-N2O m−2 y−1. Since maize grain yield under SCM with 70% N-fertilization was similar to that under Control with 100% N-fertilization, we concluded that under our experimental conditions SCM may be used for reducing N input (−30%) and N2O emissions (−23%), while contemporarily maintaining maize yield. Hence, SCM can be considered an available tool to improve agriculture’s alignment to the United Nation Sustainable Development Goals (UN SDGs) and to comply with Europe’s Farm to Fork strategy for reducing N-fertilizer inputs.
Molecular Diversity within a Mediterranean and European Panel of Tetraploid Wheat (T. turgidum subsp.) Landraces and Modern Germplasm Inferred Using a High-Density SNP Array
Authors: Ganugi Paola , Palchetti Enrico , Gori Massimo , Calamai Alessandro , Burridge Amanda , Biricolti Stefano , Benedettelli Stefano , Masoni Alberto
Published: February 2021

High-density single-nucleotide polymorphism (SNP) molecular markers are widely used to assess the genetic variability of plant varieties and cultivars, which is nowadays recognized as an important source of well-adapted alleles for environmental stresses. In our study, the genetic diversity and population genetic structure of a collection of 265 accessions of eight tetraploid Triticumturgidum L. subspecies were investigated using 35,143 SNPs screened with a 35K Axiom® array. The neighbor-joining algorithm, discriminant analysis of principal components (DAPC), and the Bayesian model-based clustering algorithm implemented in STRUCTURE software revealed clusters in accordance with the taxonomic classification, reflecting the evolutionary history of the Triticum turgidum L. subspecies and the phylogenetic relationships among them. Based on these results, a clear picture of the population structure within a collection of tetraploid wheats is given herein. Moreover, the genetic potential of landraces and wild relatives for the research of specific traits of interest is highlighted. This research provides a great contribution to future phenotyping and crossing activities. In particular, the recombination efficiency and gene selection programs aimed at developing durum wheat composite cross populations that are adapted to Mediterranean conditions could be improved.
Extraction and Analysis of Natural Product in Plant
Authors: Barbero Gerardo F.
Published: February 2021

Plants are well known for being a major source of natural compounds, many of them generally considered of biological interest for their antioxidant, anti-inflammatory, antimicrobial or anti-cancerogenic properties [...]
Emergence of Nosocomial Pneumonia Caused by Colistin-Resistant Escherichia coli in Patients Admitted to Chest Intensive Care Unit
Authors: El-Mokhtar Mohamed A. , Daef Enas , Mohamed Hussein Aliae A. R. , Hashem Maiada K. , Hassan Hebatallah M.
Published: February 2021

(1) Background: Colistin is a last-resort antibiotic used in treating multidrug-resistant Gram-negative infections. The growing emergence of colistin resistance in Escherichia coli (E. coli) represents a serious health threat, particularly to intensive care unit (ICU) patients. (2) Methods: In this work, we investigated the emergence of colistin resistance in 140 nosocomial E. coli isolated from patients with pneumonia and admitted to the chest ICU over 36 months. Virulence and resistance-related genes and E. coli pathotypes in colistin-resistant and colistin-sensitive isolates were determined. (3) Results: Colistin resistance was observed in 21/140 (15%) of the nosocomial E. coli isolates. The MIC50 of the resistant strains was 4 mg/L, while MIC90 was 16 mg/L. Colistin-resistant isolates were also co-resistant to amoxicillin, amoxicillin/clavulanic, aztreonam, ciprofloxacin, and chloramphenicol. The mechanism of colistin resistance was represented by the presence of mcr-1 in all resistant strains. Respectively, 42.9% and 36.1% of colistin-resistant and colistin-sensitive groups were extended-spectrum β-lactamase (ESBL) producers, while 23.8% and 21% were metallo β-lactamase (MBL) producers. blaTEM-type was the most frequently detected ESBL gene, while blaIMP-type was the most common MBL in both groups. Importantly, most resistant strains showed a significantly high prevalence of astA (76.2%), aggR (76.2%), and pic (52.4%) virulence-related genes. Enteroaggregative E. coli (76%) was the most frequently detected genotype among the colistin-resistant strains. (4) Conclusion: The high colistin resistance rate observed in E. coli strains isolated from patients with nosocomial pneumonia in our university hospital is worrisome. These isolates carry different drug resistance and virulence-related genes. Our results indicate the need for careful monitoring of colistin resistance in our university hospital. Furthermore, infection control policies restricting the unnecessary use of extended-spectrum cephalosporins and carbapenems are necessary.
Impact of On-Farm Interventions against CTX-Resistant Escherichia coli on the Contamination of Carcasses before and during an Experimental Slaughter
Authors: Projahn Michaela , Sachsenroeder Jana , Correia-Carreira Guido , Becker Evelyne , Martin Annett , Thomas Christian , Hobe Carolin , Reich Felix , Robé Caroline , Roesler Uwe , Kaesbohrer Annemarie , Bandick Niels
Published: February 2021

Cefotaxime (CTX)-resistant Enterobacteriaceae are still an ongoing challenge in human and veterinary health. High prevalence of these resistant bacteria is detected in broiler chickens and the prevention of their dissemination along the production pyramid is of major concern. The impact of certain on-farm interventions on the external bacterial contamination of broiler chickens, as well as their influence on single processing steps and (cross-) contamination, have not yet been evaluated. Therefore, we investigated breast skin swab samples of broiler chickens before and during slaughter at an experimental slaughter facility. Broiler chickens were previously challenged with CTX-resistant Escherichia coli strains in a seeder-bird model and subjected to none (control group (CG)) or four different on-farm interventions: drinking water supplementation based on organic acids (DW), slow growing breed Rowan × Ranger (RR), reduced stocking density (25 kg/sqm) and competitive exclusion with Enterobacteriales strain IHIT36098(CE). Chickens of RR, 25 kg/sqm, and CE showed significant reductions of the external contamination compared to CG. The evaluation of a visual scoring system indicated that wet and dirty broiler chickens are more likely a vehicle for the dissemination of CTX-resistant and total Enterobacteriaceae into the slaughterhouses and contribute to higher rates of (cross-) contamination during processing.
Sirt1 and Sirt3 Activation Improved Cardiac Function of Diabetic Rats via Modulation of Mitochondrial Function
Authors: Paramesha Bugga , Anwar Mohammed Soheb , Meghwani Himanshu , Maulik Subir Kumar , Arava Sudheer Kumar , Banerjee Sanjay K
Published: February 2021

In the present study, we aimed to evaluate the effect of Sirt1, Sirt3 and combined activation in high fructose diet-induced insulin resistance rat heart and assessed the cardiac function focusing on mitochondrial health and function. We administered the Sirt1 activator; SRT1720 (5 mg/kg, i.p.), Sirt3 activator; Oroxylin-A (10 mg/kg i.p.) and the combination; SRT1720 + Oroxylin-A (5 mg/kg and 10 mg/kg i.p.) daily from 12th week to 20th weeks of study. We observed significant perturbations of most of the cardiac structural and functional parameters in high fructose diet-fed animals. Administration of SRT1720 and Oroxylin-A improved perturbed cardiac structural and functional parameters by decreasing insulin resistance, oxidative stress, and improving mitochondrial function by enhancing mitochondrial biogenesis, OXPHOS expression and activity in high fructose diet-induced insulin-resistant rats. However, we could not observe the synergistic effect of SRT1720 and Oroxylin-A combination. Similar to in-vivo study, perturbed mitochondrial function and oxidative stress observed in insulin-resistant H9c2 cells were improved after activation of Sirt1 and Sirt3. We observed that Sirt1 activation enhances Sirt3 expression and mitochondrial biogenesis, and the opposite effects were observed after Sirt1 inhibition in cardiomyoblast cells. Taken together our results conclude that activation of Sirt1 alone could be a potential therapeutic target for diabetes-associated cardiovascular complications.
A Genetic Crow Search Algorithm for Optimization of Operation Sequencing in Process Planning
Authors: Djurdjev Mica , Cep Robert , Lukic Dejan , Antic Aco , Popovic Branislav , Milosevic Mijodrag
Published: February 2021

Computer-aided process planning represents the main link between computer-aided design and computer-aided manufacturing. One of the crucial tasks in computer-aided process planning is an operation sequencing problem. In order to find the optimal process plan, operation sequencing problem is formulated as an NP hard combinatorial problem. To solve this problem, a novel genetic crow search approach (GCSA) is proposed in this paper. The traditional CSA is improved by employing genetic strategies such as tournament selection, three-string crossover, shift and resource mutation. Moreover, adaptive crossover and mutation probability coefficients were introduced to improve local and global search abilities of the GCSA. Operation precedence graph is adopted to represent precedence relationships among features and vector representation is used to manipulate the data in the Matlab environment. A new nearest mechanism strategy is added to ensure that elements of machines, tools and tool approach direction (TAD) vectors are integer values. Repair strategy to handle precedence constraints is adopted after initialization and shift mutation steps. Minimization of total production cost is used as the optimization criterion to evaluate process plans. To verify the performance of the GCSA, two case studies with different dimensions are carried out and comparisons with traditional and some modern algorithms from the literature are discussed. The results show that the GCSA performs well for operation sequencing problem in computer-aided process planning.
Performance Evaluation of Rigid Braced Indirect Suspended Ceiling with Steel Panels
Authors: Lee Jae-Sub , Jung Dam-I , Lee Doo-Yong , Cho Bong-Ho
Published: February 2021

In Korea, the earthquakes in Gyeongju (2016) and Pohang (2017) have led to increased interest in the seismic design of nonstructural elements. Among these, the suspended ceiling can cause personal injury and property damage. In addition, most suspended ceilings that are used in Korea neither have seismic design details nor meet the current seismic design standards. There are two seismic design methods for suspended ceilings using a perimeter clip and a brace. In the United States and Japan, seismic design of ceilings is typically used, but the concepts of applying and installing braces are different. This is because the typical ceiling systems are different in the United States and Japan. In this study, a brace-applied ceiling system that is suitable for a suspended ceiling with a steel panel was applied in the indirect suspended ceiling mainly used in Korea. In addition, the seismic performance was verified through a shaking table test. All the specimens were applied with anti-falling clips that are designed to prevent the panels from falling, and they satisfy KDS 41 17 00, which is a Korean seismic design life safety standard. Without considering these factors, the performance level is lower than a nonseismic designed ceiling, which is not properly designed or constructed.
Modelling of Amplitude Modulated Vocal Fry Glottal Area Waveforms Using an Analysis-by-Synthesis Approach
Authors: Devaraj Vinod , Aichinger Philipp
Published: February 2021

The characterization of voice quality is important for the diagnosis of a voice disorder. Vocal fry is a voice quality which is traditionally characterized by a low frequency and a long closed phase of the glottis. However, we also observed amplitude modulated vocal fry glottal area waveforms (GAWs) without long closed phases (positive group) which we modelled using an analysis-by-synthesis approach. Natural and synthetic GAWs are modelled. The negative group consists of euphonic, i.e., normophonic GAWs. The analysis-by-synthesis approach fits two modelled GAWs for each of the input GAW. One modelled GAW is modulated to replicate the amplitude and frequency modulations of the input GAW and the other modelled GAW is unmodulated. The modelling errors of the two modelled GAWs are determined to classify the GAWs into the positive and the negative groups using a simple support vector machine (SVM) classifier with a linear kernel. The modelling errors of all vocal fry GAWs obtained using the modulating model are smaller than the modelling errors obtained using the unmodulated model. Using the two modelling errors as predictors for classification, no false positives or false negatives are obtained. To further distinguish the subtypes of amplitude modulated vocal fry GAWs, the entropy of the modulator’s power spectral density and the modulator-to-carrier frequency ratio are obtained.
Smart Rings vs. Smartwatches: Utilizing Motion Sensors for Gesture Recognition
Authors: Kurz Marc , Gstoettner Robert , Sonnleitner Erik
Published: February 2021

Since electronic components are constantly getting smaller and smaller, sensors and logic boards can be fitted into smaller enclosures. This miniaturization lead to the development of smart rings containing motion sensors. These sensors of smart rings can be used to recognize hand/finger gestures enabling natural interaction. Unlike vision-based systems, wearable systems do not require a special infrastructure to operate in. Smart rings are highly mobile and are able to communicate wirelessly with various devices. They could potentially be used as a touchless user interface for countless applications, possibly leading to new developments in many areas of computer science and human–computer interaction. Specifically, the accelerometer and gyroscope sensors of a custom-built smart ring and of a smartwatch are used to train multiple machine learning models. The accuracy of the models is compared to evaluate whether smart rings or smartwatches are better suited for gesture recognition tasks. All the real-time data processing to predict 12 different gesture classes is done on a smartphone, which communicates wirelessly with the smart ring and the smartwatch. The system achieves accuracy scores of up to 98.8%, utilizing different machine learning models. Each machine learning model is trained with multiple different feature vectors in order to find optimal features for the gesture recognition task. A minimum accuracy threshold of 92% was derived from related research, to prove that the proposed system is able to compete with state-of-the-art solutions.
RETRACTED: Biomaterial-Modified Magnetic Nanoparticles γ-Fe2O3, Fe3O4 Used to Improve the Efficiency of Hyperthermia of Tumors in HepG2 Model
Authors: Zhao Shang , Lee Seoksoon
Published: February 2021

The main treatments for cancer recorded to date include chemotherapy, radiotherapy, and surgery. Although we have achieved great success in treating certain types of tumors, there are still many incurable even with the help of modern treatments. Currently, the principles of magnetic-induction hyperthermia in magnetic nanoparticle hyperthermia are considered an effective treatment for cancer cells. As reported in previous articles, these nanoparticles generate a lot of heat that raises the temperatures of tumors, hence treating the cancer cells. The other significant potential of magnetic nanoparticles is the ability to combine heat and drug release for cancer treatment. However, within the biologically safe range of AC magnetic fields, the lack of induction heating power and the high criteria for biocompatibility in superparamagnetic-nanoparticle hyperthermia agents still make up the key challenges for the successful clinical application of magnetic hyperthermia. In this study, two different types of iron oxide nanoparticles (γ-Fe2O3, Fe3O4) were modified with whey protein isolate (WPI) to form bio-modified superparamagnetic nanoparticles with spherical or diamond-shaped structures and diameters between 20 and 100 nm, which demonstrate excellent stability under different conditions. Adriamycin (ADM) has also been successfully loaded onto these nanoparticles and used in this experiment. In vitro and in vivo experimental studies were performed using these WPI-modified nanoparticles on HepG2 tumor models and mice to assess their bioavailability and biological feasibility. The results prove that these WPI-modified nanoparticles perform satisfactorily in conjunction with hyperthermia to cure tumors completely.
Improve the Energy Efficiency of the Cooling System by Slide Regulating the Capacity of Refrigerator Compressors
Authors: Cárcel-Carrasco Javier , Pascual-Guillamón Manuel , Salas-Vicente Fidel
Published: February 2021

A fundamental part of the electric consumption of the main industries of the food sector comes from the refrigeration production, needed in all production phases. Therefore, every measure that aims to optimize the electric consumption and increase the efficiency of centralized industrial refrigeration systems will help the energetic waste of the company, improving reliability and maintenance. Acting on the regulation of capacity of power compressors used can be a good way to save energy. This article shows a case studied by the authors in an industrial company in the meat industry in Spain, where the refrigeration systems have a great importance in the productive process. It displays the methodology used, the description of the taken actions and the results obtained. These combined measures brought about an improvement, with an energetic saving value reaching 400 MWh per year, leading to an equivalent in CO2 emission reduction of 147.9 tons.
Few-Cycle Infrared Pulse Evolving in FEL Oscillators and Its Application to High-Harmonic Generation for Attosecond Ultraviolet and X-ray Pulses
Authors: Hajima Ryoichi
Published: February 2021

Generation of few-cycle optical pulses in free-electron laser (FEL) oscillators has been experimentally demonstrated in FEL facilities based on normal-conducting and superconducting linear accelerators. Analytical and numerical studies have revealed that the few-cycle FEL lasing can be explained in the frame of superradiance, cooperative emission from self-bunched systems. In the present paper, we review historical remarks of superradiance FEL experiments in short-pulse FEL oscillators with emphasis on the few-cycle pulse generation and discuss the application of the few-cycle FEL pulses to the scheme of FEL-HHG, utilization of infrared FEL pulses to drive high-harmonic generation (HHG) from gas and solid targets. The FEL-HHG enables one to explore ultrafast science with attosecond ultraviolet and X-ray pulses with a MHz repetition rate, which is difficult with HHG driven by solid-state lasers. A research program has been launched to develop technologies for the FEL-HHG and to conduct a proof-of-concept experiment of FEL-HHG.
Data Needs for Modeling Low-Temperature Non-Equilibrium Plasmas: The LXCat Project, History, Perspectives and a Tutorial
Authors: Carbone Emile , Graef Wouter , Hagelaar Gerjan , Boer Daan , Hopkins Matthew M. , Stephens Jacob C. , Yee Benjamin T. , Pancheshnyi Sergey , van Dijk Jan , Pitchford Leanne
Published: February 2021

Technologies based on non-equilibrium, low-temperature plasmas are ubiquitous in today’s society. Plasma modeling plays an essential role in their understanding, development and optimization. An accurate description of electron and ion collisions with neutrals and their transport is required to correctly describe plasma properties as a function of external parameters. LXCat is an open-access, web-based platform for storing, exchanging and manipulating data needed for modeling the electron and ion components of non-equilibrium, low-temperature plasmas. The data types supported by LXCat are electron- and ion-scattering cross-sections with neutrals (total and differential), interaction potentials, oscillator strengths, and electron- and ion-swarm/transport parameters. Online tools allow users to identify and compare the data through plotting routines, and use the data to generate swarm parameters and reaction rates with the integrated electron Boltzmann solver. In this review, the historical evolution of the project and some perspectives on its future are discussed together with a tutorial review for using data from LXCat.
Estimate e-Golf Battery State Using Diagnostic Data and a Digital Twin
Authors: Merkle Lukas , Pöthig Michael , Schmid Florian
Published: February 2021

Li-ion battery packs are the heart of modern electric vehicles. Due to their perishable nature, it is crucial to supervise them closely. In addition to on-board supervision over safety and range, insights into the battery’s degradation are also becoming increasingly important, not only for the vehicle manufacturers but also for vehicle users. The concept of digital twins has already emerged on the field of automotive technology, and can also help to digitalize the vehicle’s battery. In this work, we set up a data pipeline and digital battery twin to track the battery state, including State of charge (SOC) and State of Health (SOH). To achieve this goal, we reverse-engineer the diagnostics interface of a 2014 e-Golf to query for UDS messages containing both battery pack and cell-individual data. An OBD logger records the data with edge-processing capability. Pushing this data into the cloud twin system using IoT-technology, we can fit battery models to the data and infer for example, cell individual internal resistance from them. We find that the resistances of the cells differ by a magnitude of two. Furthermore, we propose an architecture for the battery twin in which the twin fleet shares resources like models by encapsulating them in Docker containers run on a cloud stack. By using web technology, we present the analyzed results on a web interface.
Influence of Circadian Rhythm in the Eye: Significance of Melatonin in Glaucoma
Authors: Martínez-Águila Alejandro , Martín-Gil Alba , Carpena-Torres Carlos , Pastrana Cristina , Carracedo Gonzalo
Published: February 2021

Circadian rhythm and the molecules involved in it, such as melanopsin and melatonin, play an important role in the eye to regulate the homeostasis and even to treat some ocular conditions. As a result, many ocular pathologies like dry eye, corneal wound healing, cataracts, myopia, retinal diseases, and glaucoma are affected by this cycle. This review will summarize the current scientific literature about the influence of circadian patterns on the eye, focusing on its relationship with increased intraocular pressure (IOP) fluctuations and glaucoma. Regarding treatments, two ways should be studied: the first one, to analyze if some treatments could improve their effect on the ocular disease when their posology is established in function of circadian patterns, and the second one, to evaluate new drugs to treat eye pathologies related to the circadian rhythm, as it has been stated with melatonin or its analogs, that not only could be used as the main treatment but as coadjutant, improving the circadian pattern or its antioxidant and antiangiogenic properties.
p53-R273H Sustains ROS, Pro-Inflammatory Cytokine Release and mTOR Activation While Reducing Autophagy, Mitophagy and UCP2 Expression, Effects Prevented by wtp53
Authors: Romeo Maria Anele , Gilardini Montani Maria Saveria , Benedetti Rossella , Arena Andrea , D’Orazi Gabriella , Cirone Mara
Published: February 2021

p53 is the most frequently mutated or inactivated gene in cancer, as its activity is not reconcilable with tumor onset and progression. Moreover, mutations in the p53 gene give rise to mutant proteins such as p53-R273H that, besides losing the wild type p53 (wtp53) capacity to safeguard genome integrity, may promote carcinogenesis, mainly due to its crosstalk with pro-oncogenic pathways. Interestingly, the activation of oncogenic pathways is interconnected with reactive oxygen species (ROS) and the release of pro-inflammatory cytokines that contribute to create an inflammatory/pro-tumorigenic milieu. In this study, based on experiments involving p53-R273H silencing and transfection, we showed that this mutant p53 (mutp53) promoted cancer cell survival by increasing intracellular ROS level and pro-inflammatory/immune suppressive cytokine release, activating mTOR, reducing autophagy and mitophagy and downregulating uncoupling protein 2 (UCP2). Interestingly, p53-R273H transfection into cancer cells carrying wtp53 induced none of these effects and resulted in p21 upregulation. This suggests that wtp53 may counteract several pro-tumorigenic activities of p53-R273H and this could explain the lower aggressiveness of cancers carrying heterozygous mutp53 in comparison to those harboring homozygous mutp53.
Rhizosphere Bacterium Rhodococcus sp. P1Y Metabolizes Abscisic Acid to Form Dehydrovomifoliol
Authors: Yuzikhin Oleg S. , Gogoleva Natalia E. , Shaposhnikov Alexander I. , Konnova Tatyana A. , Osipova Elena V. , Syrova Darya S. , Ermakova Elena A. , Shevchenko Valerii P. , Nagaev Igor Yu. , Shevchenko Konstantin V. , Myasoedov Nikolay F. , Safronova Vera I. , Shavarda Alexey L. , Nizhnikov Anton A. , Belimov Andrey A. , Gogolev Yuri V.
Published: February 2021

The phytohormone abscisic acid (ABA) plays an important role in plant growth and in response to abiotic stress factors. At the same time, its accumulation in soil can negatively affect seed germination, inhibit root growth and increase plant sensitivity to pathogens. ABA is an inert compound resistant to spontaneous hydrolysis and its biological transformation is scarcely understood. Recently, the strain Rhodococcus sp. P1Y was described as a rhizosphere bacterium assimilating ABA as a sole carbon source in batch culture and affecting ABA concentrations in plant roots. In this work, the intermediate product of ABA decomposition by this bacterium was isolated and purified by preparative HPLC techniques. Proof that this compound belongs to ABA derivatives was carried out by measuring the molar radioactivity of the conversion products of this phytohormone labeled with tritium. The chemical structure of this compound was determined by instrumental techniques including high-resolution mass spectrometry, NMR spectrometry, FTIR and UV spectroscopies. As a result, the metabolite was identified as (4RS)-4-hydroxy-3,5,5-trimethyl-4-[(E)-3-oxobut-1-enyl]cyclohex-2-en-1-one (dehydrovomifoliol). Based on the data obtained, it was concluded that the pathway of bacterial degradation and assimilation of ABA begins with a gradual shortening of the acyl part of the molecule.
Oral Squamous Cell Carcinoma Is Associated with a Low Thrombosis Risk Due to Storage Pool Deficiency in Platelets
Authors: Haen Pierre , Crescence Lydie , Mege Diane , Altié Alexandre , Dubois Christophe , Panicot-Dubois Laurence
Published: February 2021

Venous thrombo-embolism (VTE) disease is the second most common cause of mortality in cancer patients, and evaluation and prevention of thrombosis risk is essential. VTE-associated risk varies according to the type of tumor disease. Oral cancer is the most frequent type of head and neck cancer, and it represents approximately 2.1% of all cancers worldwide. Most tumors are squamous cell carcinomas and are mainly due to tobacco and alcohol abuse. VTE risk associated with oral squamous cell carcinoma (OSCC) is low. However, many studies have shown that OSCC has the following biological features of cancers associated with a high thrombosis risk: modified thrombosis and fibrinolysis mechanisms; strong expression of procoagulant proteins; secretion of procoagulant microparticles; and production of procoagulant cytokines. Using an original mouse model of tongue squamous cell carcinoma, our study aimed to clarify this paradoxical situation. First, we showed that OSCC tumors have a pro-aggregatory phenotype and a high local thrombosis risk. Second, we found that tongue tumor mice do not have an elevated systemic thrombosis risk (the risk of an “at distance” thrombosis event such as lower extremity deep venous thrombosis or pulmonary embolism) and even show a reduction in risk. Third, we demonstrated that tongue tumor mice show a reduction in platelet reactivity, which explains the low systemic thrombosis risk. Finally, we found that tongue tumor mice present granule pool deficiency, thereby explaining the reduction in platelet reactivity and systemic thrombosis risk.
Intractable Itch in Atopic Dermatitis: Causes and Treatments
Authors: Umehara Yoshie , Kiatsurayanon Chanisa , Trujillo-Paez Juan Valentin , Chieosilapatham Panjit , Peng Ge , Yue Hainan , Nguyen Hai Le Thanh , Song Pu , Okumura Ko , Ogawa Hideoki , Niyonsaba François
Published: February 2021

Itch or pruritus is the hallmark of atopic dermatitis and is defined as an unpleasant sensation that evokes the desire to scratch. It is also believed that itch is a signal of danger from various environmental factors or physiological abnormalities. Because histamine is a well-known substance inducing itch, H1-antihistamines are the most frequently used drugs to treat pruritus. However, H1-antihistamines are not fully effective against intractable itch in patients with atopic dermatitis. Given that intractable itch is a clinical problem that markedly decreases quality of life, its treatment in atopic dermatitis is of high importance. Histamine-independent itch may be elicited by various pruritogens, including proteases, cytokines, neuropeptides, lipids, and opioids, and their cognate receptors, such as protease-activated receptors, cytokine receptors, Mas-related G protein-coupled receptors, opioid receptors, and transient receptor potential channels. In addition, cutaneous hyperinnervation is partly involved in itch sensitization in the periphery. It is believed that dry skin is a key feature of intractable itch in atopic dermatitis. Treatment of the underlying conditions that cause itch is necessary to improve the quality of life of patients with atopic dermatitis. This review describes current insights into the pathophysiology of itch and its treatment in atopic dermatitis.
Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune Interactions
Authors: Liu Tracy W. , Gammon Seth T. , Fuentes David , Piwnica-Worms David
Published: February 2021

A major obstacle in studying the interplay between cancer cells and the immune system has been the examination of proposed biological pathways and cell interactions in a dynamic, physiologically relevant system in vivo. Intravital imaging strategies are one of the few molecular imaging techniques that can follow biological processes at cellular resolution over long periods of time in the same individual. Bioluminescence imaging has become a standard preclinical in vivo optical imaging technique with ever-expanding versatility as a result of the development of new emission bioluminescent reporters, advances in genomic techniques, and technical improvements in bioluminescence imaging and processing methods. Herein, we describe an advance of technology with a molecular imaging window chamber platform that combines bioluminescent and fluorescent reporters with intravital macro-imaging techniques and bioluminescence spectral unmixing in real time applied to heterogeneous living systems in vivo for evaluating tumor signaling dynamics and immune cell enzyme activities concurrently.
Myopic Progression in Girls with Gonadotrophin-Releasing Hormone Agonist Treatment for Central Precocious Puberty
Authors: Chung Seung Ah , Lee Hae Sang , Kim Seung Woo , Hwang Jin Soon
Published: February 2021

We sought to determine whether the myopic progression of patients with central precocious puberty (CPP) who were undergoing treatment differed from that of their healthy peers with normal pubertal onset and progression. Eighteen girls with CPP and 14 age-matched controls who underwent regular ophthalmic examinations for at least 1 year were included. All the CPP patients received a 3.75 mg leuprolide acetate depot subcutaneously every 28 days. The spherical equivalent (SE) and axial length (AL) for myopia progression and the pubertal parameters (height, body weight, body mass index, Tanner stage, and bone age) were compared between the two groups. Of 32 subjects with a mean age of 8.6 ± 0.7 years, the SEs and ALs did not differ at baseline between the two groups, which had similar weight and similar body mass index. After 1 year, both the CPP patients and controls showed myopic progression, with an average myopic shift of −0.73 ± 0.48 diopters (D) and AL elongation with a mean change of 0.44 ± 0.61 mm. The SE and AL changes over 1 year were greater in the controls than those in the CPP patients, which was not statistically significant (–0.85 ± 0.55 D vs. –0.64 ± 0.41 D and 0.55 ± 0.89 mm vs. 0.35 ± 0.22 mm, respectively). The change in AL correlated significantly with the change in the height (β = 0.691, p = 0.039). In this 1-year study, the CPP patients with treatments trended to show less myopic progression than the controls.
Optimal Fractional-Order Active Disturbance Rejection Controller Design for PMSM Speed Servo System
Authors: Chen Pengchong , Luo Ying , Peng Yibing , Chen Yangquan
Published: February 2021

In this paper, a fractional-order active disturbance rejection controller (FOADRC), combining a fractional-order proportional derivative (FOPD) controller and an extended state observer (ESO), is proposed for a permanent magnet synchronous motor (PMSM) speed servo system. The global stable region in the parameter (Kp, Kd, μ)-space corresponding to the observer bandwidth ωo can be obtained by D-decomposition method. To achieve a satisfied tracking and anti-load disturbance performance, an optimal ADRC tuning strategy is proposed. This tuning strategy is applicable to both FOADRC and integer-order active disturbance rejection controller (IOADRC). The tuning method not only meets user-specified frequency-domain indicators but also achieves a time-domain performance index. Simulation and experimental results demonstrate that the proposed FOADRC achieves better speed tracking, and more robustness to external disturbance performances than traditional IOADRC and typical Proportional-Integral-Derivative (PID) controller. For example, the JITAE for speed tracking of the designed FOADRC are less than 52.59% and 55.36% of the JITAE of IOADRC and PID controller, respectively. Besides, the JITAE for anti-load disturbance of the designed FOADRC are less than 17.11% and 52.50% of the JITAE of IOADRC and PID controller, respectively.
Trade-offs between Error Exponents and Excess-Rate Exponents of Typical Slepian–Wolf Codes
Authors: Tamir (Averbuch) Ran , Merhav Neri
Published: February 2021

Typical random codes (TRCs) in a communication scenario of source coding with side information in the decoder is the main subject of this work. We study the semi-deterministic code ensemble, which is a certain variant of the ordinary random binning code ensemble. In this code ensemble, the relatively small type classes of the source are deterministically partitioned into the available bins in a one-to-one manner. As a consequence, the error probability decreases dramatically. The random binning error exponent and the error exponent of the TRCs are derived and proved to be equal to one another in a few important special cases. We show that the performance under optimal decoding can be attained also by certain universal decoders, e.g., the stochastic likelihood decoder with an empirical entropy metric. Moreover, we discuss the trade-offs between the error exponent and the excess-rate exponent for the typical random semi-deterministic code and characterize its optimal rate function. We show that for any pair of correlated information sources, both error and excess-rate probabilities exponential vanish when the blocklength tends to infinity.
A Vehicle-Model-Aided Navigation Reconstruction Method for a Multicopter during a GPS Outage
Authors: Xu Yifan , Zhang Qian , Zhang Jingjuan , Wang Xueyun , Yu Zelong
Published: February 2021

The integrated navigation of inertial navigation systems (INS) and the Global Positioning System (GPS) is essential for small unmanned aerial vehicles (UAVs) such as multicopters, providing steady and accurate position, velocity, and attitude information. Nevertheless, decreasing navigation accuracy is a serious threat to flight safety due to the long-term drift error of INS in the absence of GPS measurements. To bridge the GPS outage for multicopters, this paper proposes a novel navigation reconstruction method for small multicopters, which combines the vehicle dynamic model and micro-electro-mechanical system (MEMS) sensors. Firstly, an induced drag model is introduced into the dynamic model of the vehicle, and an efficient online parameter identification method is designed to estimate the model parameters quickly. Secondly, the body velocity can be calculated from the vehicle model and accelerometer measurement. In addition, the nongravitational acceleration estimated from body velocity and radar height are utilized to yield a more accurate attitude estimate. Fusing the information of the attitude, body velocity, magnetic heading, and radar height, a navigation system based on an error-state Kalman filter is reconstructed. Then, an adaptive measurement covariance algorithm based on a fuzzy logic system is designed to reduce the weight due to the disturbed acceleration. Finally, the hardware-in-loop experiment is carried out to demonstrate the effectiveness of the proposed method. Simulation results show that the proposed navigation reconstruction algorithm aided by the vehicle model can significantly improve navigation accuracy during a GPS outage.