bluuebunny/crossref_metadata_2025 huggingface dataset:
Searching for Long-Period Binary Central Stars of Planetary Nebulae with SALT HRS
Authors: Miszalski B. , Manick R. , Mikołajewska J. , Iłkiewicz K. , Kamath D. , Van Winckel Hans
Published: August 2019

In the last decade great strides have been made in understanding the role of binary stars in the evolution and shaping of planetary nebulæ (PNe). Observational efforts have mainly focused on finding close binaries with orbital periods of 1 day or less. Those close binary systems make up around 1 in 5 PNe, and constitute the youngest accessible window into the aftermath of the critical and unobserved common-envelope (CE) phase of binary-star evolution. The poster focused on our recent work with the High Resolution Spectrograph (HRS) on the Southern African Large Telescope (SALT) to search for long-period binaries in PNe. Considerably less is known about such long-period binaries with orbital periods of weeks to years, but they may be fundamental to improving CE population synthesis models and for determining the total binary fraction of PNe. The queue-mode operation of SALT and the excellent sensitivity and stability of HRS (which is enclosed in a vacuum tank) are ideally suited to detecting binaries with low radial-velocity amplitudes over the expected timescales of weeks to years. Many exciting new discoveries about binaries have already been made in this newly-accessible southern horizon in time-domain astronomy thanks to the many unique advantages of SALT.
Companion(s) of the Eclipsing Binary KIC 3832716
Authors: Parimucha Š. , Fedurco M. , Gajdoš P.
Published: August 2019

Detailed analyses of observations by the Kepler satellite may reveal unknown facts about objects that were previously regarded as eclipsing binaries. We present results of our analysis of such an object, KIC 3832716. We show that the system actually contains two eclipsing binaries (EB1 and EB2), with orbital periods of 1.14 and 2.17 days, orbiting around their common centre of mass with period of at least 1400 days and with an estimated mass ratio of 0.7 ± 0.3. Analyses of the (O–C) diagrams of both eclipsing pairs show three different types of variation: (i) long-term changes probably due to light-time effects, (ii) spikes caused by the superposition of the eclipses of both binaries, and (iii) semi-regular variations in EB1 with a period of 57 days, presumably caused by the presence of spots on its secondary component.
Detecting AGB stars in LG Dwarf Galaxies for Understanding Galaxy Formation and Evolution
Authors: Saremi E. , Javadi A. , van Loon J. , Khosroshahi H. , Torki M.
Published: August 2019

Stars are the main ingredients of galaxies, and the sites of the creation of most chemical elements in our universe. The knowledge that we gain from studying nearby resolved stellar populations assists directly our ability to measure the properties of distant galaxies. The overall objective of this project is to study galaxy formation and evolution in a complete environment of the dwarf galaxies in the Local Group, by using the same methods for all of them. For that purpose, we used the INT to conduct a monitoring survey of the majority of Local-Group dwarf galaxies in order to identify the most evolved AGB stars that are long-period variables (LPV). LPV stars reach their maximum brightness amplitudes at optical wavelengths, owing to changes in temperature. They trace stellar populations as young as ∼30 Myr up to as old as ∼10 Gyr, and identifying them is one of the best ways of reconstructing star-formation history using a method that we have developed and applied successfully to other Local-Group galaxies. Since the luminosity variations span 100–1000 days, we planned observations over 10 epochs, spaced ∼3 months apart; 9 epochs of data have so far been obtained.
Accurate Photometry with Digitized Photographic Plates of the Moscow Collection
Authors: Sokolovsky K. V. , Zubareva A. M. , Kolesnikova D. M. , Samus N. N. , Antipin S. V. , Belinski A. A.
Published: August 2019

Photographic plate archives contain a wealth of information about the positions and brightness of celestial objects decades ago. Plate digitization is necessary to make this information accessible, but extracting it is a technical challenge. We have developed algorithms to extract photometry with an accuracy of better than ∼0.1 mag. in the range 13 < B < 17 mag from photographic images obtained in 1948–1996 with the 40-cm Sternberg Institute astrograph (30 × 30 cm plate size, 10 × 10 deg field of view) and digitized using a flatbed scanner. The extracted photographic light-curves are used to identify thousands of new high-amplitude variable stars (>0.2 mag). The algorithms are implemented in the free software VaST available at http://scan.sai.msu.ru/vast/
One-dimensional beam-steering Fabry–Perot cavity (FPC) antenna with a reconfigurable superstrate
Authors: Ji Lu-Yang , Fu Shuai , Zhang Lin-Xi , Li Jian-Ying
Published: August 2019

In this work, a new reconfigurable discrete 1D beam-steering Fabry–Perot cavity antenna with enhanced radiation performance is presented. It consists of a probe-fed patch antenna printed on the ground plane and a reconfigurable metasurface acting as the upper partially reflective surface to realize beam steering. By utilizing 6 × 6 proposed reconfigurable unit cells on the superstrate, the beam-steering angle can be effectively enhanced from ±7° to ±17° with fewer active elements and a much simpler biasing network. The proposed antenna was fabricated to validate the feasibility. Good agreement between the simulated and measured results is achieved. Moreover, the measured realized gains are over 11 dBi with a gain variation from the boresight direction to the tilted direction <0.2 dBi.
Evaluation of herbicides for hair fescue (Festuca filiformis) management and potential seedbank reduction in lowbush blueberry
Authors: White Scott N.
Published: August 2019

Hair fescue is a widespread, seed-limited perennial grass in lowbush blueberry fields. Growers rely on pronamide, an expensive and difficult herbicide to use, for hair fescue management. Recent herbicide registrations provide opportunity to reduce pronamide use, though effects of these herbicides on hair fescue suppression and seedbank reduction are not well understood. The objectives of this research were to determine (1) the effects of herbicides currently registered in lowbush blueberry on suppression of hair fescue tufts and (2) whether suppression of hair fescue with these herbicides reduces hair fescue seedbanks. Pronamide gave the most consistent reductions in flowering tuft density, though applications after both autumn pruning and autumn of the nonbearing year were required to reduce the hair fescue seedbank by >60% across sites. Nonbearing-year hexazinone applications did not control hair fescue or reduce the seedbank. Nonbearing-year terbacil applications reduced flowering tuft density, but hair fescue recovered in the bearing year, and the seedbank was not reduced. Glufosinate applications following autumn pruning or in the spring of the nonbearing year did not suppress hair fescue or reduce the seedbank. Spring nonbearing-year foramsulfuron applications, alone or after autumn or spring glufosinate applications, reduced hair fescue flowering tuft density, but hair fescue recovered in the bearing year, and the seedbank was not reduced. In contrast, autumn and spring glufosinate applications followed by spring nonbearing-year foramsulfuron applications, when combined with autumn nonbearing-year pronamide applications, reduced flowering tuft density in both the nonbearing and bearing years and reduced the hair fescue seedbank by 58% to 83% across sites. Results indicate that hair fescue seedbanks can be reduced in lowbush blueberry fields and that a reduction in pronamide use will require alternative bearing-year treatments to prevent tuft recovery and seed production.
Inducing Unconscious Stress
Authors: van der Ploeg Melanie M. , Brosschot Jos F. , Quirin Markus , Lane Richard D. , Verkuil Bart
Published: August 2019

Abstract. Stress-related stimuli may be presented outside of awareness and may ultimately influence health by causing repetitive increases in physiological parameters, such as blood pressure (BP). In this study, we aimed to corroborate previous studies that demonstrated BP effects of subliminally presented stress-related stimuli. This would add evidence to the hypothesis that unconscious manifestations of stress can affect somatic health. Additionally, we suggest that these findings may be extended by measuring affective changes relating to these physiological changes, using measures for self-reported and implicit positive and negative affectivity. Using a repeated measures between-subject design, we presented either the prime word “angry” ( n = 26) or “relax” ( n = 28) subliminally (17 ms) for 100 trials to a student sample and measured systolic and diastolic BP, heart rate (HR), and affect. The “angry” prime, compared to the “relax” prime, did not affect any of the outcome variables. During the priming task, a higher level of implicit negative affect (INA) was associated with a lower systolic BP and diastolic BP. No association was found with HR. Self-reported affect and implicit positive affect were not related to the cardiovascular (CV) activity. In sum, anger and relax primes elicited similar CV activity patterns, but implicit measures of affect may provide a new method to examine the relationship between (unconscious) stress and health.
Distinguishing TBI Malingering and Fatigue Using Event-Related Potentials
Authors: Robinson Lindsey K. , McFadden Sandra L.
Published: August 2019

Abstract. Poorer-than-expected performance on cognitive-behavioral tasks may indicate malingering, or it could be an outcome of fatigue, resulting in false positives when suboptimal task performance is used to flag individuals feigning or exaggerating symptoms of traumatic brain injury (TBI). The primary goal of this study was to examine the P3 event-related potentials (ERP) and behavioral outcomes associated with TBI malingering and fatigue, in order to distinguish between them. A secondary goal was to determine if history of TBI (hTBI) is associated with differences in fatigue, ERPs, or performance on a short-term memory task. Participants completed the Mental Fatigue and Related Symptoms (SR-MF) questionnaire and were interviewed to assess TBI history, then they completed a computerized “old/new” (match-mismatch) task while ERPs were recorded, under three conditions: Normal, Malinger, and Fatigue. Participants reported mild fatigue at the end of study, with no difference between individuals reporting a history of TBI ( n = 32) and healthy controls ( n = 47). Fatigue was associated with prolonged P3 latency but was otherwise indistinguishable from Normal. In contrast, Malinger was clearly distinguished from Normal by significantly lower accuracy, longer reaction times, reduced P3 amplitude on Match trials, and a smaller old/new ERP effect. Individuals with a history of TBI reported clinical levels of fatigue at baseline but did not differ significantly from healthy controls on any behavioral or ERP measure. The results support the use of behavioral and ERP measures to identify malingering, without concern over confounding effects of mild subjective fatigue, including mild fatigue induced by testing.
Robustness of Statistical Power in Group-Randomized Studies of	Mediation Under an Optimal Sampling Framework
Authors: Cox Kyle , Kelcey Benjamin
Published: August 2019

Abstract. When planning group-randomized studies probing mediation, effective and efficient sample allocation is governed by several parameters including treatment-mediator and mediator-outcome path coefficients and the mediator and outcome intraclass correlation coefficients. In the design stage, these parameters are typically approximated using information from prior research and these approximations are likely to deviate from the true values eventually realized in the study. This study investigates the robustness of statistical power under an optimal sampling framework to misspecified parameter values in group-randomized designs with group- or individual-level mediators. The results suggest that estimates of statistical power are robust to misspecified parameter values across a variety of conditions and tests. Relative power remained above 90% in most conditions when the incorrect parameter value ranged between 50% and 150% of the true parameter.
Mantle Dynamics of the Eastern Mediterranean and Middle East: Constraints From P‐Wave Anisotropic Tomography
Authors: Wei Wei , Zhao Dapeng , Wei Feixiang , Bai Xiang , Xu Jiandong
Published: August 2019

High‐resolution images of P‐wave anisotropic tomography beneath the Eastern Mediterranean and Middle East are determined by inverting a large number of high‐quality P‐wave arrival times. Our results clearly show along‐strike variations of subducting slabs in the Hellenic subduction zone. At least three high‐velocity anomalies are imaged beneath and behind the north Hellenides, which are associated with the subducting Hellenic slab at different stages, while in regions further south, a single slab is revealed that has subducted down to a depth of ~1,100 km. The slab feature is not prominent in the Cyprus subduction zone, but a fine‐scale high‐velocity structure is revealed beneath the central Anatolia, which may reflect the detached Cyprus slab. Trench‐parallel fast‐velocity directions occur in the forearc mantle wedge of the Hellenic subduction zone, which may reflect mantle flow induced by slab curvature. In the backarc north Aegean and westernmost Anatolia, dominant NNE‐SSW to N‐S fast‐velocity directions exist in the shallow mantle, which accord well with the extensional direction of present deformation at the surface, suggesting a vertically coherent deformation in the lithosphere. Our results reveal a large‐scale mantle flow beneath western Arabia, which is possibly related to the Zagros and Caucasus orogens and westward motion of the Anatolia lithosphere. The Arabian lithospheric plate is thinner beneath the Zagros than that under the Mesopotamian Foredeep, suggesting that a large portion of the crust was scrapped off from the subducted Arabian lithosphere during the Zagros orogen.
A Time‐Dependent Model of Elastic Stress in the Central Apennines, Italy
Authors: Caporali A. , Zurutuza J. , Bertocco M.
Published: August 2019

Seismicity in the Central Apennines is characterized by normal faulting with dip NE‐SW near 45°. If the stress at the hypocenter of the 2016 Norcia (Mw= 6.5) and 2009 L'Aquila (Mw= 6.3 on the Paganica fault) earthquakes originated only from stress transfer from previous historical events, the orientation of the principal stress axes would have been inconsistent with the observed tensional regime. The additional contribution of a regional stress is thus required, but Global Navigation Satellite System geodesy provides only stress rates. We empirically estimate a time multiplier for the regional stress rate, computed with a dense Global Navigation Satellite System network, such that the principal stress axes resulting from the sum of the stress transferred by previous events and the regional stress rate multiplied by the empirical temporal scale are consistent with normal faulting, both at the L'Aquila and Norcia hypocenters. Based on a Catalogue of 36 events of magnitude larger than 5.6, we estimate the total Coulomb stress at depths and along planes parallel to those of L'Aquila and Norcia. We provide evidence of an asymmetry of the Coulomb stress leading to a stress concentration near the hypocenter of the two events just prior of the 2009 and 2016 earthquakes. This stress anomaly disappeared after the two events. Similar stress patterns are observed for earlier events, which took place in 1461 at L'Aquila, 1703 on the Montereale plain, and in 1703 at Norcia/Valnerina. The 1997 sequence of Colfiorito exhibits a similar, anisotropic Coulomb stress pattern. Other areas with a similar stress anisotropy could be seismic gaps.
Tidal Distortions Related to Extreme Atmospheric Forcing Over the Inner Shelf
Authors: Paniagua‐Arroyave Juan F. , Valle‐Levinson Arnoldo , Parra Sabrina M. , Adams Peter N.
Published: August 2019

Inner shelf transport is driven by the combination of tides, waves, density gradients, and wind that interact with bottom bedforms. To extend understanding of shoal‐swale bathymetry influences on inner‐shelf hydrodynamics, we provide observations at a swale ~13 m deep within Cape Canaveral shoals, ~36 km away from Hurricane Matthew's path during October 2016. Observations document a unidirectional flow that reached 2.7 m/s at a distance ~15 km away from the shoreline. The flow changed direction, over the course of the storm, counterclockwise from southwestward (~2.7 m/s) to northeastward (~1 m/s). The unidirectional flow modified tides over the inner shelf analogously to a river discharge in a channelized tidal flow, producing overtides of the semidiurnal tidal harmonic. Our results provide measurements during extreme conditions related to shore parallel‐propagating hurricanes that can inform morphodynamic and ecological models.
Genome-wide association study for proliferative diabetic retinopathy in Africans
Authors: Liu Chang , Chen Guanjie , Bentley Amy R. , Doumatey Ayo , Zhou Jie , Adeyemo Adebowale , Yang Jinkui , Rotimi Charles
Published: August 2019

Proliferative diabetic retinopathy (PDR) is a sight-threatening complication of diabetes that is associated with longer duration of diabetes and poor glycemic control under a genetic susceptibility background. Although GWAS of PDR have been conducted in Europeans and Asians, none has been done in continental Africans, a population at increased risk for PDR. Here, we report a GWAS of PDR among Africans. PDR cases (n = 64) were T2D patients with neovascularization in the retina and/or retinal detachment. Controls (n = 227) were T2D patients without listed eye complications despite high risk (T2D duration ≥10 years and fasting blood glucose >169 mg/dl). Replication was assessed in African Americans enrolled in the ARIC study. We identified 4 significant loci: WDR72, HLA-B, GAP43/RP11-326J18.1, and AL713866.1. At WDR72 the most strongly associated SNPs were rs12906891 (MAF = 0.071; p = 9.68 × 10-10; OR = 1.46, 95% CI [1.30,1.64]) and rs11070992 (MAF = 0.14; p = 4.23 × 10−8; OR = 1.28, 95%CI [1.17–1.40]). rs11070992 replicated in African Americans (p = 0.04). Variants in this gene have been associated with diabetic retinopathy, glycemic control, revascularization, and kidney disease.
UASOL, a large-scale high-resolution outdoor stereo dataset
Authors: Bauer Zuria , Gomez-Donoso Francisco , Cruz Edmanuel , Orts-Escolano Sergio , Cazorla Miguel
Published: August 2019

In this paper, we propose a new dataset for outdoor depth estimation from single and stereo RGB images. The dataset was acquired from the point of view of a pedestrian. Currently, the most novel approaches take advantage of deep learning-based techniques, which have proven to outperform traditional state-of-the-art computer vision methods. Nonetheless, these methods require large amounts of reliable ground-truth data. Despite there already existing several datasets that could be used for depth estimation, almost none of them are outdoor-oriented from an egocentric point of view. Our dataset introduces a large number of high-definition pairs of color frames and corresponding depth maps from a human perspective. In addition, the proposed dataset also features human interaction and great variability of data, as shown in this work.
NLRP3 inflammasome is attenuated in patients with Mycobacterium avium complex lung disease and correlated with decreased interleukin-1β response and host susceptibility
Authors: Wu Ming-Fang , Shu Chin-Chung , Wang Jann-Yuan , Yan Bo-Shiun , Lai Hsin-Chih , Chiang Bor-Luen , Wu Lawrence Shih-Hsin , Yu Chong-Jen
Published: August 2019

The incidence of nontuberculous mycobacteria lung disease (NTM-LD) is increasing in patients without human immunodeficiency virus. Mycobacterium avium complex (MAC) is one of the most common pathogenic species. The presence of MAC has a clinical relevance of around 35~42%, indicating the possibility of host susceptibility. Previous studies have shown that interleukin (IL)-1β and IL-1-receptor knock-out mice are susceptible to mycobacterial infections; however, the role of inflammasome-driven interleukin (IL)-1β has not been studied in MAC-LD. We enrolled patients with MAC-LD and healthy controls. Peripheral blood mononuclear cells (PBMCs), monocytes, and monocyte-derived macrophages were stimulated by MAC bacilli. The responses of interleukin(IL)-1β and the expression of inflammasome and toll-like receptors (TLRs) were measured. Single nucleotide polymorphisms (SNPs) were also examined for NLRP3 and TLR2 genes. In the patients with MAC-LD, the IL-1β responses decreased in PBMCs, monocytes, and macrophages assayed by MAC bacilli in comparison to the healthy controls. In addition, the level of caspase-1 after stimulation was lower in the MAC-LD group, although the mRNA level of IL-1β was not significantly lower. In surveying the activation of IL-1β, the MAC-LD group had an attenuated mRNA level of NLRP3 but similar levels of AIM2 and ASC compared with the controls. The SNPs rs3806268 and rs34298354 in NLRP3 for females and rs3804100 in TLR2 for males were associated with MAC-LD. In conclusion, our patients with MAC-LD had attenuated IL-1β production, which may have been due to lower activation of the NLRP3-caspase-1 axis. Two SNPs of NLRP3 and one of TLR2 were correlated with MAC-LD, possibly indicating host susceptibility.
Network analysis of canine brain morphometry links tumour risk to oestrogen deficiency and accelerated brain ageing
Authors: Rzechorzek Nina M. , Saunders Olivia M. , Hiscox Lucy V. , Schwarz Tobias , Marioni-Henry Katia , Argyle David J. , Schoenebeck Jeffrey J. , Freeman Tom C.
Published: August 2019

Structural ‘brain age’ is a valuable but complex biomarker for several brain disorders. The dog is an unrivalled comparator for neurological disease modeling, however canine brain morphometric diversity creates computational and statistical challenges. Using a data-driven approach, we explored complex interactions between patient metadata, brain morphometry, and neurological disease. Twenty-four morphometric parameters measured from 286 canine brain magnetic resonance imaging scans were combined with clinical parameters to generate 9,438 data points. Network analysis was used to cluster patients according to their brain morphometry profiles. An ‘aged-brain’ profile, defined by a small brain width and volume combined with ventriculomegaly, was revealed in the Boxer breed. Key features of this profile were paralleled in neutered female dogs which, relative to un-neutered females, had an 11-fold greater risk of developing brain tumours. Boxer dog and geriatric dog groups were both enriched for brain tumour diagnoses, despite a lack of geriatric Boxers within the cohort. Our findings suggest that advanced brain ageing enhances brain tumour risk in dogs and may be influenced by oestrogen deficiency—a risk factor for dementia and brain tumours in humans. Morphometric features of brain ageing in dogs, like humans, might better predict neurological disease risk than patient chronological age.
Prophage protein RacR activates lysozyme LysN, causing the growth defect of E. coli JM83
Authors: Tang Qiongwei , Feng Meilin , Hou Bingbing , Ye Jiang , Wu Haizhen , Zhang Huizhan
Published: August 2019

Prophage enriched the prokaryotic genome, and their transcriptional factors improved the protein expression network of the host. In this study, we uncovered a new prophage-prophage interaction in E. coli JM83. The Rac prophage protein RacR (GenBank accession no. AVI55875.1) directly activated the transcription of φ80dlacZΔM15 prophage lysozyme encoding gene 19 (GenBank accession no. ACB02445.1, renamed it lysN, lysozyme nineteen), resulting in the growth defect of JM83. This phenomenon also occurred in DH5α, but not in BL21(DE3) and MG1655 due to the genotype differences. However, deletion of lysN could not completely rescued JM83 from the growth arrest, indicating that RacR may regulate other related targets. In addition, passivation of RacR regulation was found in the late period of growth of JM83, and it was transmissible to daughter cells. Altogether, our study revealed part of RacR regulatory network, which suggested some advanced genetic strategies in bacteria.
NaCl induced salt adaptive changes and enhanced accumulation of 20-hydroxyecdysone in the in vitro shoot cultures of Spinacia oleracea (L.)
Authors: Muchate Niramaya S. , Rajurkar Nilima S. , Suprasanna Penna , Nikam Tukaram D.
Published: August 2019

Spinach (Spinacia oleracea L.) is a vegetable plant with high nutritional properties. In the present work, we studied responses of in vitro shoot cultures to salt stress (0 (control), 100, 200 and 300 mM NaCl) and salt stress-induced accumulation of 20-hydroxyecdysone (20E). Our results revealed that effect of low to moderate level of salinity stress (100–200 mM) was less pronounced on growth and tissue water content (TWC) of shoot cultures compared to higher salinity level (300 mM). The salt treated shoot cultures showed better osmotic adjustment in terms of significant accumulation of compatible solutes and total soluble sugars and also higher antioxidant enzyme activity. As the NaCl stress was increased, there was a corresponding linear raise in the Na+ accumulation while the contents of both K+ and Ca2+ decreased significantly. We also studied salt-stress induced accumulation of a bioactive compound; 20E and results showed that 200 mM salt treated shoot cultures accumulated significantly 2.9 fold higher 20E as compared to untreated shoot cultures. The results suggest that Spinacia oleracea exhibits considerable salt tolerance with better osmotic adjustment and can be considered a suitable candidate for the production of bioactive secondary metabolite.
Role of fundoplication in treatment of patients with symptoms of hiatal hernia
Authors: Li Zhi-tong , Ji Feng , Han Xin-wei , Yuan Li-li , Wu Zheng-yang , Xu Miao , Peng De-lu , Wang Zhong-gao
Published: August 2019

Gastroesophageal reflux disease (GERD) is often associated with hiatal hernia (HH). However, the need for fundoplication during hiatal hernia repair (HHR) remains controversial. The objective of this study was to evaluate the effect of HHR with concomitant laparoscopic Nissen fundoplication (HHR-LNF) in HH patients. A total of 122 patients with symptomatic HH were randomized to receive either HHR (n = 61) or HHR-LNF (n = 61). The measures of evaluating outcomes included DeMeester scores (DMS), complications, Reflux Diagnostic Questionnaire and patients’ satisfaction 24 months following surgery. Despite comparable values in both groups at randomization, the DMS, total numbers of reflux episodes and percentage of time with pH < 4 were significantly higher in HHR group than in HHR-LNF group (P = 0.017, P = 0.002 and P = 0.019, respectively) at 6 months after surgery. One months postoperatively, complications were higher in the HHR-LNF group than in the HHR group (all P < 0.001), and there was no difference between the two groups at 6 months. By the end of the 2-year follow-up, HHR-LNF group showed a significantly lower reflux syndrome frequency-intensity score and greater percentage of satisfaction compared with HHR group (all P < 0.001). Laparoscopic HHR should be combined with a fundoplication in GERD patients with HH. HHR-LNF is safe and effective, not only improve reflux-related symptom, but also reduce the incidence of complications.
Self-reduction of the native TiO2 (110) surface during cooling after thermal annealing – in-operando investigations
Authors: Rogala M. , Bihlmayer G. , Dabrowski P. , Rodenbücher C. , Wrana D. , Krok F. , Klusek Z. , Szot K.
Published: August 2019

We investigate the thermal reduction of TiO2 in ultra-high vacuum. Contrary to what is usually assumed, we observe that the maximal surface reduction occurs not during the heating, but during the cooling of the sample back to room temperature. We describe the self-reduction, which occurs as a result of differences in the energies of defect formation in the bulk and surface regions. The findings presented are based on X-ray photoelectron spectroscopy carried out in-operando during the heating and cooling steps. The presented conclusions, concerning the course of redox processes, are especially important when considering oxides for resistive switching and neuromorphic applications and also when describing the mechanisms related to the basics of operation of solid oxide fuel cells.
Extreme levels of fallout radionuclides and other contaminants in glacial sediment (cryoconite) and implications for downstream aquatic ecosystems
Authors: Owens Philip N. , Blake William H. , Millward Geoffrey E.
Published: August 2019

Glaciers in most parts of the world are retreating, releasing water and sediments to downstream rivers. Studies have found elevated levels of fallout radionuclides (FRNs) and other contaminants in glacial sediments, especially cryoconite, in European glaciers and Greenland. However, there are no equivalent studies for glaciers in North America. We report concentrations of FRNs (i.e. 137Cs, 210Pbun and 241Am) and other contaminants (i.e. metal(loids), phosphorus) in cryoconite and proglacial sediments from a glacier in British Columbia, Canada, and compare values to suspended sediments from the downstream river. The mean concentrations of 137Cs, 210Pbun and 241Am in cryoconite were 2,123 ± 74, 7,535 ± 224 and 11.5 ± 3.0 Bq kg−1, respectively, which are an order of magnitude greater than those for most soils and surficial materials. FRNs were much lower in suspended sediments and decreased with distance away from the glacier. Geochemical elements were enriched in cryoconite relative to local clastic materials and upper continental crust. Concentrations of FRNs in cryoconite were correlated with organic matter, which suggests this is important in controlling the scavenging of hydrophobic contaminants in glacial meltwater. Low concentrations of FRNs and contaminants in suspended sediments suggest that glacial meltwater and the delivery of cryoconite have limited impact on downstream aquatic ecosystems.
Evaluation of uterine receptivity after gonadotropin releasing hormone agonist administration as an oocyte maturation trigger: a rodent model
Authors: Ezoe Kenji , Murata Nana , Yabuuchi Akiko , Kobayashi Tamotsu , Kato Keiichi
Published: August 2019

In natural cycle or minimal stimulation cycle IVF, buserelin acetate (buserelin), a gonadotropin-releasing hormone agonist, is often used as a maturation trigger; however, its effect on pregnancy outcomes remains unclear. Therefore, in the present study, we compared uterine receptivity in buserelin-administered mice with that in human chorionic gonadotropin (hCG)-administered mice during the peri-implantation period. Implantation, decidualisation, and term-pregnancy were impaired following hCG, but not buserelin administration. hCG stimulated the synthesis and secretion of progesterone and oestradiol, whereas ovarian steroidogenesis in the buserelin-treated group was comparable with that in the control group. Furthermore, similar to the observation in controls, the buserelin-treated group exhibited activation of progesterone receptor signalling and inhibition of oestrogen receptor signalling in the endometrial epithelium on the day of implantation. However, epithelial progesterone signalling was not detected, and a high expression of genes downstream to oestrogen was observed on day 4 following hCG administration. These results suggest that buserelin administration does not impact uterine receptivity as it did not affect ovarian steroidogenesis and endometrial steroid signalling. Therefore, buserelin is preferred as an oocyte maturation trigger to optimise uterine receptivity during treatments involving timed intercourse, intrauterine insemination, or fresh embryo transfer following in vitro fertilisation.
A high-throughput screen to identify novel synthetic lethal compounds for the treatment of E-cadherin-deficient cells
Authors: Beetham Henry , Chen Augustine , Telford Bryony J. , Single Andrew , Jarman Kate E. , Lackovic Kurt , Luxenburger Andreas , Guilford Parry
Published: August 2019

The cell-cell adhesion protein E-cadherin (CDH1) is a tumor suppressor that is required to maintain cell adhesion, cell polarity and cell survival signalling. Somatic mutations in CDH1 are common in diffuse gastric cancer (DGC) and lobular breast cancer (LBC). In addition, germline mutations in CDH1 predispose to the autosomal dominant cancer syndrome Hereditary Diffuse Gastric Cancer (HDGC). One approach to target cells with mutations in specific tumor suppressor genes is synthetic lethality. To identify novel synthetic lethal compounds for the treatment of cancers associated with E-cadherin loss, we have undertaken a high-throughput screening campaign of ~114,000 lead-like compounds on an isogenic pair of human mammary epithelial cell lines – with and without CDH1 expression. This unbiased approach identified 12 novel compounds that preferentially harmed E-cadherin-deficient cells. Validation of these compounds using both real-time and end-point viability assays identified two novel compounds with significant synthetic lethal activity, thereby demonstrating that E-cadherin loss creates druggable vulnerabilities within tumor cells. In summary, we have identified novel synthetic lethal compounds that may provide a new strategy for the prevention and treatment of both sporadic and hereditary LBC and DGC.
Nitrogen-based gas molecule adsorption of monolayer phosphorene under metal functionalization
Authors: Lei Shuangying , Gao Ran , Sun Xiaolong , Guo Sijia , Yu Hong , Wan Neng , Xu Feng , Chen Jie
Published: August 2019

Using the first-principles calculation based on density functional theory (DFT), the adsorption properties of nitrogen-based gases molecules (NH3, NO, NO2) on various metal (Li, Na, K, Rb, Cs, Ca, Sr, Ba, Ni, La, Tl) decorated phosphorene systems have been studied systematically. The results show that all metal decorations can improve the adsorption strength of phosphorene to nitrogen-based gases molecules except for Tl decoration. Especially, the adsorption energy of NH3 molecule on Ni decorated phosphorene is 1.305 eV, and the adsorption energies of NO and NO2 on La decorated phosphorene can be up to 2.475 and 3.734 eV, respectively. In addition, after NO and NO2 adsorptions, the electronic and magnetic properties of some metal decorated phosphorenes change, indicating that the metal decorated phosphorenes have great potential in NO and NO2 detection.
A major sea-level drop briefly precedes the Toarcian oceanic anoxic event: implication for Early Jurassic climate and carbon cycle
Authors: Krencker François-Nicolas , Lindström Sofie , Bodin Stéphane
Published: August 2019

Sea-level change is an important parameter controlling the expansion of oxygen-depleted conditions in neritic settings during oceanic anoxic events (OAEs). Despite this fundamental role, it remains on a short timescale (<1 Myr) one of the least constrained parameters for numerous OAEs. Here we present sedimentological and geochemical evidence from Morocco and East Greenland showing that a forced regression shortly precedes (ca.102 kyr) the major transgression associated with the Toarcian OAE. The forced regression can be correlated over distances greater than 3000 km in numerous Tethyan and Boreal basins, indicating that the relative sea-level change was driven by eustastic fluctuations. The major amplitude (>50 m) and short duration of the forced regression suggests that it was most likely related to the transient waxing and waning of polar ice sheet. We suggest that this short-lived glaciation might have a genetic link with the inception of the Toarcian OAE. Indeed, during the deglaciation and the accompanying sea-level rise, the thawing permafrost may have released important quantities of methane into the atmosphere that would have contributed to the Toarcian OAE rapid warming and its characteristic negative carbon isotope excursion. This study offers a hypothesis on how some hyperthermal events might be rooted in short-lived “cold-snap” episodes.
Respiration of permeabilized cardiomyocytes from mice: no sex differences, but substrate-dependent changes in the apparent ADP-affinity
Authors: Karro Niina , Laasmaa Martin , Vendelin Marko , Birkedal Rikke
Published: August 2019

Sex differences in cardiac physiology are getting increased attention. This study assessed whether isolated, permeabilized cardiomyocytes from male and female C57BL/6 mice differ in terms of their respiration with multiple substrates and overall intracellular diffusion restriction estimated by the apparent ADP-affinity of respiration. Using respirometry, we recorded 1) the activities of respiratory complexes I, II and IV, 2) the respiration rate with substrates fuelling either complex I, II, or I + II, and 3) the apparent ADP-affinity with substrates fuelling complex I and I + II. The respiration rates were normalized to protein content and citrate synthase (CS) activity. We found no sex differences in CS activity (a marker of mitochondrial content) normalized to protein content or in any of the respiration measurements. This suggests that cardiomyocytes from male and female mice do not differ in terms of mitochondrial respiratory capacity and apparent ADP-affinity. Pyruvate modestly lowered the respiration rate, when added to succinate, glutamate and malate. This may be explained by intramitochondrial compartmentalization caused by the formation of supercomplexes and their association with specific dehydrogenases. To our knowledge, we show for the first time that the apparent ADP-affinity was substrate-dependent. This suggests that substrates may change or regulate intracellular barriers in cardiomyocytes.
Prediction of Site Preference of Implanted Transition Metal Dopants in Rock-salt Oxides
Authors: Misra Debolina , Yadav Satyesh K.
Published: August 2019

Transition metals (TMs) implanted in oxides with rock-salt crystal structures (for example MgO and BaO) are assumed to substitute cations (Mg in case of MgO) from the lattice sites. We show that not all implanted TMs substitute cations but can be stable in interstitial sites as well. Stability of TM (Sc–Zn) dopants in various charge states in MgO and BaO has been investigated in the framework of density functional theory. We propose an effective way to calculate stability of implanted metals that let us predict site preference (interstitial or substitution) of the dopant in the host. We find that two factors govern the preference for an interstitial site: (i) relative ionic radius and (ii) relative oxygen affinity of cation and the TM dopants. If the radius of the cation is much larger than TM dopant, as in BaO, TM atoms always sit at interstitial sites. On the other hand, if the radius of the cation is comparable to that of the dopant TM, as in case of MgO, the transition of the preferred defect site, from substituting lattice Mg atom (Sc to Mn) to occupying interstitial site (Fe to Zn) is observed. This transition can be attributed to the change in the oxygen affinity of the TM atoms from Sc to Zn. Our results also explain experiments on Ni and Fe atoms implanted in MgO. TM dopants at interstitial sites could show substantially different and new properties from substitutionally doped stable compounds.
Compartmentalization of the proteasome-interacting proteins during sperm capacitation
Authors: Zigo Michal , Manaskova-Postlerova Pavla , Jonakova Vera , Kerns Karl , Sutovsky Peter
Published: August 2019

Ubiquitination is a stable, reversible posttranslational modification of target proteins by covalent ligation of the small chaperone protein ubiquitin. Most commonly ubiquitination targets proteins for degradation/recycling by the 26S proteasome in a well-characterized enzymatic cascade. Studies using human and non-human mammalian spermatozoa revealed the role of the ubiquitin-proteasome system (UPS) in the regulation of fertilization, including sperm-zona pellucida (ZP) interactions as well as the early events of sperm capacitation, the remodeling of the sperm plasma membrane and acrosome, and for the acquisition of sperm fertilizing ability. The present study investigated the activity of UPS during in vitro capacitation of fresh boar spermatozoa in relation to changes in sperm proteome. Parallel and sequential treatments of ejaculated and capacitated spermatozoa under proteasome permissive/inhibiting conditions were used to isolate putative sperm proteasome-associated sperm proteins in a compartment-specific manner. A differential proteomic approach employing 1D PAGE revealed differences in accumulated proteins at the molecular weights of 60, 58, 49, and 35 kDa, and MS analysis revealed the accumulation of proteins previously reported as proteasome co-purifying proteins, as well as some novel proteins. Among others, P47/lactadherin, ACRBP, ADAM5, and SPINK2 (alias SAAI) were processed by the proteasome in a capacitation dependent manner. Furthermore, the capacitation-induced reorganization of the outer acrosomal membrane was slowed down in the presence of proteasomal inhibitors. These novel results support the proposed role of UPS in sperm capacitation and open several new lines of inquiry into sperm capacitation mechanism.
Summer high temperature extremes over Northeastern China predicted by spring soil moisture
Authors: Zhang Jingyong , Yang Zhanmei , Wu Lingyun , Yang Kai
Published: August 2019

Current seasonal climate predictions mainly reside in the ocean anomalies. However, the prediction skills are generally limited over many extra-tropical land areas where the oceanic effects are relatively weak. In this study, we address the potential of preceding spring soil moisture condition to predict summer hot days over Northeastern China, a typical Northern Hemisphere mid-latitude region. The results show that spring soil moisture condition over Central-Eastern China is closely related with following summer hot days over Northeastern China for the period of 1979–2017. The statistical model based on the preceding spring soil moisture condition yields temporal cross-validated correlation skill of 0.57 for summer hot days over Northeastern China. The spatial pattern correlation skills of independent hindcast experiments for 2009–2017 are also high, ranging from 0.87 to 0.94. Our results can be easily applied to practical prediction of summer hot days over Northeastern China, and help to provide better climate services and reduce the detrimental effects of extreme heat over this extra-tropical region.
Impact of Resolution of Hyponatremia on Neurocognitive and Motor Performance in Geriatric Patients
Authors: Brinkkoetter Paul Thomas , Grundmann Franziska , Ghassabeh Panteha Jazayeri , Becker Ingrid , Johnsen Marc , Suaréz Victor , Schulz Ralf-Joachim , Streichert Thomas , Burst Volker
Published: August 2019

This observational study investigated the impact of hyponatremia resolution on the results of a comprehensive geriatric assessment (CGA) in 150 patients with age ≥70 years and serum sodium <130 mEq/L. The test battery including Barthel index of Activities of Daily Living (ADL) and various tests of neurocognitive function, motor performance and mood stability was applied on admission and at discharge. Changes of individual test results (Δ) were analyzed and normonatremic patients matched for age, gender, and ADL served as reference group. Most CGA test results improved. The improvement was more pronounced in the hyponatremia group with respect to ADL (ΔADL: 14.3 ± 17.1 vs. 9.8 ± 14.7; p = 0.002) and MMSE (ΔMMSE: 1.8 ± 3.0 vs. 0.7 ± 1.9; p = 0.002). Effect sizes were small (i.e., >0.2) in the overall analysis for ΔADL and ΔMMSE and moderate (i.e., >0.5) for ΔMMSE in the euvolemic subgroup. Beneficial effects on ΔADL and ΔMMSE were only observed in the subgroup of patients in which [Na+] was raised by >5 mEq/L and multivariable linear regression analysis confirmed [Na+] increase to be an independent predictor of MMSE improvement. Resolution of hyponatremia has a beneficial impact on the geriatric patients’ overall functional status, in particular in euvolemic cases.
18F-FDG Positron-Emission Tomography/Computed Tomography Findings of Radiographic Lesions Suggesting Old Healed Pulmonary Tuberculosis and High-risk Signs of Predicting Recurrence: A Retrospective Study
Authors: Ji Yu , Shao Chunchun , Cui Yong , Shao Guangrui , Zheng Jingsong
Published: August 2019

Pulmonary tuberculosis (PTB) is a common worldwide infection with high mortality and morbidity, especially in developing countries. This study analyzed PET/CT findings in tumor patients with radiographic lesions suggesting old healed pulmonary tuberculosis (OHPTB) and imaging follow-up to find and verify PET/CT signs that may predict tuberculosis recurrence. A retrospective analysis of the tumor patients was carried out. These patients underwent 18F-FDG PET/CT in our center from 2010 to 2018. Confirmation of tuberculosis recurrence was obtained by follow-up of morphological changes in old lesions by PET/CT or CT. In total, 238 patients with a complete medical history were included in the final study, and 22 patients experienced OHPTB recurrence. We found that the SUVmax of tuberculosis in PET/CT was significantly increased in the recurrence group compared to the non-recurrence group [5.00 (3.40, 7.30) vs. 1.10 (0.80, 1.30), P < 0.001]. The ROC curve showed good discrimination, with an AUC of 0.980, and a cut-off SUVmax value of 2.15 was identified (the sensitivity was 90.5%, the specificity was 97.2%, the positive predictive value was 76.0%, and the negative predictive value was 99.1%). Both the tumor and the anti-tumor treatment can cause the patient to be immunocompromised and might further cause the recurrence of OHPTB. Positive imaging on 18F-FDG PET can predict the recurrence of OHPPT. Although there might be a false positive, 18F-FDG PET can greatly narrow the monitoring range. A negative result on imaging has high reliability for eliminating the possibility of tuberculosis recurrence. PET/CT has important clinical significance in tuberculosis management in patients with concurrent OHPTB.
Carcinogenic risk of human papillomavirus (HPV) genotypes and potential effects of HPV vaccines in Korea
Authors: Park Eunhyang , Kim Ji-Ye , Choi Sangjoon , Kim Dae Shick , Oh Young Lyun
Published: August 2019

This study investigated the distribution of HPV types in Korean women and evaluated the carcinogenic risk of individual HPV types and the potential effects of HPV vaccines. A total of 4,081 HPV-positive samples between 2014 and 2017 were included. The most prevalent genotypes were HPV 16, 58, 68, and 56. Among them, HPV 16 was significantly higher in high-grade squamous intraepithelial neoplasia or worse (HSIL+ ) group. In cytologically evaluating the risk for HSIL+ by individual HPV types, HPV 16 was associated with the highest risk of HSIL+ (OR = 10.82; 95% CI: 7.93–14.77), followed by HPV 33, 31, 52, 18, 58, 51, and 35, in descending order (OR = 3.50 [type 33] to 2.62 [type 35]). Among those types, HPV 16, 18, 31, 33, and 58 were also significantly associated with HSIL+ on histologic evaluation. The analysis of the HPV subgroups covered by the different vaccines revealed that the HPV types covered by the 9-valent vaccine had a high association with HSIL+ (OR = 4.09; 95% CI: 3.02–5.54). Our findings highlight the different carcinogenic risks posed by the high risk HPV genotypes and the positive potential effects of the 9-valent HPV vaccine in reducing HPV-associated cervical cancer in Korea.