bluuebunny/crossref_metadata_2025 huggingface dataset:
SOVEREIGN CREDIT RISK, MACROECONOMIC DYNAMICS, AND FINANCIAL CONTAGION: EVIDENCE FROM JAPAN
Authors: Qian Zongxin , Wang Wendun , Ji Kan
Published: October 2017

We try to understand the nature of Japan's sovereign credit risk by examining the interaction between Japan's sovereign credit default swap (CDS) spreads and its financial indicators of macroeconomic fundamentals. We consider potential contagion from the global financial market and allow for reverse causality between CDS spreads and macroeconomic fundamentals. We find strong evidence of contagion from global stock markets to Japan's credit market when Lehman Brothers collapsed, whereas the European sovereign debt crisis only had temporary effects. We also show that several credit events, such as the 2011 Tohoku earthquake and rating cuts by rating agencies, significantly raised volatility in Japan's sovereign CDS market.
Exploring perceptions of the Mexican sugar-sweetened beverage tax among adolescents in north-west Mexico: a qualitative study
Authors: Ortega-Avila Ana G , Papadaki Angeliki , Jago Russell
Published: October 2017

Objective

To explore awareness and perceptions of the sugar-sweetened beverage (SSB) tax implemented in Mexico in 2014 among a sample of Mexican adolescents, and to investigate how the tax has affected their purchases and intake of SSB.

Design

Qualitative.

Setting

Semi-structured interviews were conducted in April–May 2016. The data were analysed using thematic analysis.

Subjects

Adolescents residing in north-west Mexico (n29, 55·2 % females), aged 15–19 years.

Results

Four main themes emerged: awareness of taxation; perceptions of how the tax has affected SSB intake; reasons why the tax was not perceived to have affected SSB intake; and preferences for substitution of the taxed SSB. Participants were mostly unaware of the tax and perceived that it would not cause reductions in their intake of SSB; they felt that the price increase was low and insufficient to affect intake. Taste preferences and ‘addiction’ to SSB were highlighted as the main reasons why participants perceived taxation would not affect intake. If SSB prices were to increase further via a higher tax, participants would consider substituting SSB with other beverages, namely home-made drinks (e.g. 100 % fruit juices), non-caloric, instant-flavoured drinks and water.

Conclusions

These findings provide important insights into the views of this sample of Mexican adolescents regarding the taxation of SSB, by pointing out several possible limitations of the tax policy in Mexico. These results could inform the design of future interventions directed at Mexican youth that would complement and strengthen the current SSB taxation.
WAS CONDORCET A STOIC? ROUSSEAU, UNIVERSAL EDUCATION AND RATIONAL AUTONOMY IN THE FRENCH ENLIGHTENMENT
Authors: ADKINS G. MATTHEW
Published: October 2017

This article considers the philosophical foundations of the French Enlightenment through a close study of the Stoic influences on the Marquis de Condorcet's education philosophy. The article argues that although Condorcet did not acknowledge any direct Stoic influence, his philosophy of education nevertheless should be understood in the eclectic idiom of eighteenth-century Stoic discourse. Furthermore, Condorcet's Stoicism was entirely compatible with Rousseau's Stoicism to the degree that one could call Condorcet a Rousseauean at least in matters of education theory—even though Condorcet, a protégé of Voltaire, is usually presented as a critic of Rousseau. Finally, the article suggests that the notion of liberty that Condorcet seeks to make flourish through his national education plan is in line with the Stoic ideal of rational autonomy, despite Condorcet's insistence that the modern idea of liberty (that is, his own idea) is fundamentally different from the ancient idea.
Probing Magma Reservoirs to Improve Volcano Forecasts
Authors: Lowenstern Jacob , Sisson Thomas , Hurwitz Shaul
Published: October 2017

The roots of volcanoes remain enigmatic, largely because geophysical and petrological models remain rudimentary. Scientific drilling and exploration can help.
Erratum: Genome-wide common and rare variant analysis provides novel insights into clozapine-associated neutropenia
Authors: Legge S E , Hamshere M L , Ripke S , Pardinas A F , Goldstein J I , Rees E , Richards A L , Leonenko G , Jorskog L F ,   Clozapine-Induced Agranulocytosis Consortium, Chambert K D , Collier D A , Genovese G , Giegling I , Holmans P , Jonasdottir A , Kirov G , McCarroll S A , MacCabe J H , Mantripragada K , Moran J L , Neale B M , Stefansson H , Rujescu D , Daly M J , Sullivan P F , Owen M J , O'Donovan M C , Walters J T R
Published: October 2017

Correction to: Molecular Psychiatry (2017) 22: 1502-1508; advance online publication, 12 July 2017; doi: 10.1038/mp.2016.97 In the first paragraph of the Results section and Figure 1, the authors incorrectly referred to the finding of SNP rs77897117. The correct SNP is rs77897177. The corrected figure appears in previous page.
Complex fault interaction controls continental rifting
Authors: Naliboff John B. , Buiter Susanne J. H. , Péron-Pinvidic Gwenn , Osmundsen Per Terje , Tetreault Joya
Published: October 2017

Rifted margins mark a transition from continents to oceans and contain in their architecture a record of their rift history. Recent investigations of rift architecture have suggested that multiphase deformation of the crust and mantle lithosphere leads to the formation of distinct margin domains. The processes that control transitions between these domains, however, are not fully understood. Here we use high-resolution numerical simulations to show how structural inheritance and variations in extension velocity control the architecture of rifted margins and their temporal evolution. Distinct domains form as extension velocities increase over time and deformation focuses along lithosphere-scale detachment faults, which migrate oceanwards through re-activation and complex linkages of prior fault networks. Our models demonstrate, in unprecedented detail, how faults formed in the earliest phases of continental extension control the subsequent structural evolution and complex architecture of rifted margins through fault interaction processes, hereby creating the widely observed distinct margin domains.
Post-transcriptional gene silencing mediated by microRNAs is controlled by nucleoplasmic Sfpq
Authors: Bottini Silvia , Hamouda-Tekaya Nedra , Mategot Raphael , Zaragosi Laure-Emmanuelle , Audebert Stephane , Pisano Sabrina , Grandjean Valerie , Mauduit Claire , Benahmed Mohamed , Barbry Pascal , Repetto Emanuela , Trabucchi Michele
Published: October 2017

There is a growing body of evidence about the presence and the activity of the miRISC in the nucleus of mammalian cells. Here, we show by quantitative proteomic analysis that Ago2 interacts with the nucleoplasmic protein Sfpq in an RNA-dependent fashion. By a combination of HITS-CLIP and transcriptomic analyses, we demonstrate that Sfpq directly controls the miRNA targeting of a subset of binding sites by local binding. Sfpq modulates miRNA targeting in both nucleoplasm and cytoplasm, indicating a nucleoplasmic commitment of Sfpq-target mRNAs that globally influences miRNA modes of action. Mechanistically, Sfpq binds to a sizeable set of long 3′UTRs forming aggregates to optimize miRNA positioning/recruitment at selected binding sites, including let-7a binding to Lin28A 3′UTR. Our results extend the miRNA-mediated post-transcriptional gene silencing into the nucleoplasm and indicate that an Sfpq-dependent strategy for controlling miRNA activity takes place in cells, contributing to the complexity of miRNA-dependent gene expression control.
Multi-dimensional genomic analysis of myoepithelial carcinoma identifies prevalent oncogenic gene fusions
Authors: Dalin Martin G. , Katabi Nora , Persson Marta , Lee Ken-Wing , Makarov Vladimir , Desrichard Alexis , Walsh Logan A. , West Lyndsay , Nadeem Zaineb , Ramaswami Deepa , Havel Jonathan J. , Kuo Fengshen , Chadalavada Kalyani , Nanjangud Gouri J. , Ganly Ian , Riaz Nadeem , Ho Alan L. , Antonescu Cristina R. , Ghossein Ronald , Stenman Göran , Chan Timothy A. , Morris Luc G. T.
Published: October 2017

Myoepithelial carcinoma (MECA) is an aggressive salivary gland cancer with largely unknown genetic features. Here we comprehensively analyze molecular alterations in 40 MECAs using integrated genomic analyses. We identify a low mutational load, and high prevalence (70%) of oncogenic gene fusions. Most fusions involve the PLAG1 oncogene, which is associated with PLAG1 overexpression. We find FGFR1-PLAG1 in seven (18%) cases, and the novel TGFBR3-PLAG1 fusion in six (15%) cases. TGFBR3-PLAG1 promotes a tumorigenic phenotype in vitro, and is absent in 723 other salivary gland tumors. Other novel PLAG1 fusions include ND4-PLAG1; a fusion between mitochondrial and nuclear DNA. We also identify higher number of copy number alterations as a risk factor for recurrence, independent of tumor stage at diagnosis. Our findings indicate that MECA is a fusion-driven disease, nominate TGFBR3-PLAG1 as a hallmark of MECA, and provide a framework for future diagnostic and therapeutic research in this lethal cancer.
Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors
Authors: Kleinjan Dirk A. , Wardrope Caroline , Nga Sou Si , Rosser Susan J.
Published: October 2017

The nuclease-deactivated variant of CRISPR-Cas9 proteins (dCas9) fused to heterologous transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene expression in mammalian cells. In such a system the long-term presence of a stable dCas9 effector can be a draw-back precluding the ability to switch rapidly between repressed and activated target gene expression states, imposing a static environment on the synthetic regulatory circuits in the cell. To address this issue we have generated a toolkit of conditionally degradable or stabilisable orthologous dCas9 or Cpf1 effector proteins, thus opening options for multidimensional control of functional activities through combinations of orthogonal, drug-tunable artificial transcription factors.
Combining chemical and genetic approaches to increase drought resistance in plants
Authors: Cao Min-Jie , Zhang Yu-Lu , Liu Xue , Huang Huan , Zhou X. Edward , Wang Wen-Long , Zeng Ai , Zhao Chun-Zhao , Si Tong , Du Jiamu , Wu Wen-Wu , Wang Fu-Xing , Xu H. Eric , Zhu Jian-Kang
Published: October 2017

Drought stress is a major threat to crop production, but effective methods to mitigate the adverse effects of drought are not available. Here, we report that adding fluorine atoms in the benzyl ring of the abscisic acid (ABA) receptor agonist AM1 optimizes its binding to ABA receptors by increasing the number of hydrogen bonds between the compound and the surrounding amino acid residues in the receptor ligand-binding pocket. The new chemicals, known as AMFs, have long-lasting effects in promoting stomatal closure and inducing the expression of stress-responsive genes. Application of AMFs or transgenic overexpression of the receptor PYL2 in Arabidopsis and soybean plants confers increased drought resistance. The greatest increase in drought resistance is achieved when AMFs are applied to the PYL2-overexpression transgenic plants. Our results demonstrate that the combining of potent chemicals with transgenic overexpression of an ABA receptor is very effective in helping plants combat drought stress.
Algorithm for post-clustering curation of DNA amplicon data yields reliable biodiversity estimates
Authors: Frøslev Tobias Guldberg , Kjøller Rasmus , Bruun Hans Henrik , Ejrnæs Rasmus , Brunbjerg Ane Kirstine , Pietroni Carlotta , Hansen Anders Johannes
Published: October 2017

DNA metabarcoding is promising for cost-effective biodiversity monitoring, but reliable diversity estimates are difficult to achieve and validate. Here we present and validate a method, called LULU, for removing erroneous molecular operational taxonomic units (OTUs) from community data derived by high-throughput sequencing of amplified marker genes. LULU identifies errors by combining sequence similarity and co-occurrence patterns. To validate the LULU method, we use a unique data set of high quality survey data of vascular plants paired with plant ITS2 metabarcoding data of DNA extracted from soil from 130 sites in Denmark spanning major environmental gradients. OTU tables are produced with several different OTU definition algorithms and subsequently curated with LULU, and validated against field survey data. LULU curation consistently improves α-diversity estimates and other biodiversity metrics, and does not require a sequence reference database; thus, it represents a promising method for reliable biodiversity estimation.
Suppression of mRNAs encoding CD63 family tetraspanins from the carcinogenic liver fluke Opisthorchis viverrini results in distinct tegument phenotypes
Authors: Chaiyadet Sujittra , Krueajampa Watchara , Hipkaeo Wiphawi , Plosan Yada , Piratae Supawadee , Sotillo Javier , Smout Michael , Sripa Banchob , Brindley Paul J. , Loukas Alex , Laha Thewarach
Published: October 2017

The liver fluke Opisthorchis viverrini infects 10 million people in Southeast Asia and causes cholangiocarcinoma (CCA). Fluke secreted and tegumental proteins contribute to the generation of a tumorigenic environment and are targets for drug and vaccine-based control measures. Herein, we identified two tetraspanins belonging to the CD63 family (Ov-TSP-2 and Ov-TSP-3) that are abundantly expressed in the tegument proteome of O. viverrini. Ov-tsp-2 and tsp-3 transcripts were detected in all developmental stages of O. viverrini. Protein fragments corresponding to the large extracellular loop (LEL) of each TSP were produced in recombinant form and antibodies were raised in rabbits. Ov-TSP-2 and TSP-3 were detected in whole worm extracts and excretory/secretory products of O. viverrini and reacted with sera from infected hamsters and humans. Antibodies confirmed localization of Ov-TSP-2 and TSP-3 to the adult fluke tegument. Using RNA interference, Ov-tsp-2 and tsp-3 mRNA expression was significantly suppressed for up to 21 days in vitro. Ultrastructural observation of tsp-2 and tsp-3 dsRNA-treated flukes resulted in phenotypes with increased tegument thickness, increased vacuolation (tsp-2) and reduced electron density (tsp-3). These studies confirm the importance of CD63 family tegument tetraspanins in parasitic flukes and support efforts to target these proteins for vaccine development.
Synthesis of graphene/DPA composite for determination of nicotine in tobacco products
Authors: Jing Yanqiu , Yu Baohua , Li Penghui , Xiong Bin , Cheng Yuyuan , Li Yaoguang , Li Chunguang , Xiao Xianyi , Chen Mengqi , Chen Liangyuan , Zhang Yu , Zhao Mingqin , Cheng Chuance
Published: October 2017

In this contribution, the azo dye (E)-1-(4-((4-(phenylamino)phenyl)diazenyl) phenyl)ethanone (DPA) was combined with reduced graphene oxide (RGO) for the electrochemical modification of a pencil graphite electrode (RGO/DPA/PGE) surface. A series of electrochemical measurements were used for the characterization of the modified electrode surfaces. At the modified electrode, nicotine was irreversibly reduced. An obvious increase was observed in the reductive peak current of nicotine at the modified electrode, indicating the capability of the RGO/DPA composite to increase the electron transfer rate. The current was found proportional to the nicotine concentration in a range of 31 to 1900 μM, and the limit of detection (LOD) was calculated as 7.6 μM.
Reinstatement of memory representations for lifelike events over the course of a week
Authors: Oedekoven Christiane S. H. , Keidel James L. , Berens Sam C. , Bird Chris M.
Published: October 2017

When we remember an event, the content of that memory is represented across the brain. Detailed memory retrieval is thought to involve the reinstatement of those representations. Functional MRI combined with representational similarity analyses (RSA) of spatial patterns of brain activity has revealed reinstatement of recently-experienced events throughout a core memory retrieval network. In the present study, participants were scanned while they watched, immediately retrieved and then retrieved after a week, 24 short videos. Following the delayed retrieval, they freely recalled all videos outside of the scanner. We observed widespread within- and between-subject reinstatement effects within a posterior midline core memory retrieval network during all phases of the experiment. Within precuneus, bilateral middle temporal gyrus and the left hippocampus, reinstatement effects between the retrieval phases correlated with memory performance. These findings extend previous studies that have only employed short retention periods or highly rehearsed materials, demonstrating that memory representations for unique events are reliably reinstated over longer timeframes that are meaningful in the context of real-world episodic memory.
A-ZIP53, a dominant negative reveals the molecular mechanism of heterodimerization between bZIP53, bZIP10 and bZIP25 involved in Arabidopsis seed maturation
Authors: Jain Prateek , Shah Koushik , Sharma Nishtha , Kaur Raminder , Singh Jagdeep , Vinson Charles , Rishi Vikas
Published: October 2017

In Arabidopsis, maturation phase, an intricate process in seed formation is tightly regulated by the DNA binding activity of protagonist basic leucine zipper 53 (bZIP53) transcription factor and its heterodimerizing partners, bZIP10 and bZIP25. Structural determinants responsible for heterodimerization specificity of bZIP53 are poorly understood. Analysis of amino acid sequences of three bZIPs does not identify interactions that may favor heterodimerization. Here, we describe a designed dominant negative termed A-ZIP53 that has a glutamic acid-rich amphipathic peptide sequence attached to N-terminal of bZIP53 leucine zipper. Circular dichroism (CD) and mass spectrometry studies with equimolar mixture of three bZIP proteins in pairs showed no heterodimer formation whereas A-ZIP53 interacted and formed stable heterodimers with bZIP53, bZIP10, and bZIP25. A-ZIP53 electrostatically mimics DNA and can overcome repulsion between basic DNA binding regions of three bZIP proteins. Gel shift experiments showed that A-ZIP53 can inhibit the DNA binding of three proteins. CD studies demonstrated the specificity of A-ZIP53 as it did not interact with bZIP39 and bZIP72. Transient co-transfections in Arabidopsis protoplasts showed that A-ZIP53 inhibited three bZIPs and their putative heterodimers-mediated transactivation of GUS reporter gene. Furthermore, four newly designed acidic extensions were evaluated for their ability to interact with three bZIPs.
MIF Inhibitor ISO-1 Protects Photoreceptors and Reduces Gliosis in Experimental Retinal Detachment
Authors: Kim Bongsu , Kusibati Rania , Heisler-Taylor Tyler , Mantopoulos Dimosthenis , Ding Jiaxi , Abdel-Rahman Mohamed H. , Satoskar Abhay R. , Godbout Jonathan P. , Bhattacharya Sanjoy K. , Cebulla Colleen M.
Published: October 2017

Photoreceptor death and retinal gliosis underlie the majority of vision threatening retinal diseases including retinal detachment (RD). Although the underlying pathobiology of vision limiting processes in RD is not fully understood, inflammation is known to play a critical role. We conducted an iTRAQ proteomic screen of up- and down-regulated proteins in a murine model of RD to identify potential targetable candidates. Macrophage migration inhibitory factor (MIF) was identified and evaluated for neurotoxic and pro-gliotic effects during RD. Systemic administration of the MIF inhibitor ISO-1 significantly blocked photoreceptor apoptosis, outer nuclear layer (ONL) thinning, and retinal gliosis. ISO-1 and MIF knockout (MIFKO) had greater accumulation of Müller glia pERK expression in the detached retina, suggesting that Müller survival pathways might underlie the neuroprotective response. Our data show the feasibility of the MIF-inhibitor ISO-1 to block pathological damage responses in retinal detachment and provide a rationale to explore MIF inhibition as a potential therapeutic option for RD.
Polyphyletic ancestry of expanding Patagonian Chinook salmon populations
Authors: Correa Cristian , Moran Paul
Published: October 2017

Chinook salmon native to North America are spreading through South America’s Patagonia and have become the most widespread anadromous salmon invasion ever documented. To better understand the colonization history and role that genetic diversity might have played in the founding and radiation of these new populations, we characterized ancestry and genetic diversity across latitude (39–48°S). Samples from four distant basins in Chile were genotyped for 13 microsatellite loci, and allocated, through probabilistic mixture models, to 148 potential donor populations in North America representing 46 distinct genetic lineages. Patagonian Chinook salmon clearly had a diverse and heterogeneous ancestry. Lineages from the Lower Columbia River were introduced for salmon open-ocean ranching in the late 1970s and 1980s, and were prevalent south of 43°S. In the north, however, a diverse assembly of lineages was found, associated with net-pen aquaculture during the 1990s. Finally, we showed that possible lineage admixture in the introduced range can confound allocations inferred from mixture models, a caveat previously overlooked in studies of this kind. While we documented high genetic and lineage diversity in expanding Patagonian populations, the degree to which diversity drives adaptive potential remains unclear. Our new understanding of diversity across latitude will guide future research.
Incidence and mortality of lung cancer: global trends and association with socioeconomic status
Authors: Wong Martin C. S. , Lao Xiang Qian , Ho Kin-Fai , Goggins William B. , Tse Shelly L. A.
Published: October 2017

We examined the correlation between lung cancer incidence/mortality and country-specific socioeconomic development, and evaluated its most recent global trends. We retrieved its age-standardized incidence rates from the GLOBOCAN database, and temporal patterns were assessed from global databases. We employed simple linear regression analysis to evaluate their correlations with Human Development Index (HDI) and Gross Domestic Product (GDP) per capita. The average annual percent changes (AAPC) of the trends were evaluated from join-point regression analysis. Country-specific HDI was strongly correlated with age-standardized incidence (r = 0.70) and mortality (r = 0.67), and to a lesser extent GDP (r = 0.24 to 0.55). Among men, 22 and 30 (out of 38 and 36) countries showed declining incidence and mortality trends, respectively; whilst among women, 19 and 16 countries showed increasing incidence and mortality trends, respectively. Among men, the AAPCs ranged from −2.8 to −0.6 (incidence) and −3.6 to −1.1 (mortality) in countries with declining trend, whereas among women the AAPC range was 0.4 to 8.9 (incidence) and 1 to 4.4 (mortality) in countries with increasing trend. Among women, Brazil, Spain and Cyprus had the greatest incidence increase, and all countries in Western, Southern and Eastern Europe reported increasing mortality. These findings highlighted the need for targeted preventive measures.
Co-Culture of Plant Beneficial Microbes as Source of Bioactive Metabolites
Authors: Vinale F. , Nicoletti R. , Borrelli F. , Mangoni A. , Parisi O. A. , Marra R. , Lombardi N. , Lacatena F. , Grauso L. , Finizio S. , Lorito M. , Woo S. L.
Published: October 2017

In microbial cultures the production of secondary metabolites is affected by experimental conditions, and the discovery of novel compounds is often prevented by the re-isolation of known metabolites. To limit this, it is possible to cultivate microorganisms by simulating naturally occurring interactions, where microbes co-exist in complex communities. In this work, co-culturing experiments of the biocontrol agent Trichoderma harzianum M10 and the endophyte Talaromyces pinophilus F36CF have been performed to elicit the expression of genes which are not transcribed in standard laboratory assays. Metabolomic analysis revealed that the co-culture induced the accumulation of siderophores for both fungi, while production of M10 harzianic and iso-harzianic acids was not affected by F36CF. Conversely, metabolites of the latter strain, 3-O-methylfunicone and herquline B, were less abundant when M10 was present. A novel compound, hereby named harziaphilic acid, was isolated from fungal co-cultures, and fully characterized. Moreover, harzianic and harziaphilic acids did not affect viability of colorectal cancer and healthy colonic epithelial cells, but selectively reduced cancer cell proliferation. Our results demonstrated that the co-cultivation of plant beneficial fungi may represent an effective strategy to modulate the production of bioactive metabolites and possibly identify novel compounds.
Sweetness induces sleep through gustatory signalling independent of nutritional value in a starved fruit fly
Authors: Hasegawa Tatsuya , Tomita Jun , Hashimoto Rina , Ueno Taro , Kume Shoen , Kume Kazuhiko
Published: October 2017

Starvation reduces sleep in various animal species including humans and fruit flies. Immediate hunger and the following insufficient nutritional status resulting from starvation may affect sleep and arousal differently. In order to clarify the mechanism underlying the relationship between diet and sleep, we analysed the sleep behaviour of Drosophila melanogaster that were either starved or fed with different types of sugars. Starved flies showed longer activity bouts, short sleep bouts and a decreased arousal threshold. Non-nutritive sweeteners such as sucralose and arabinose, which are sweet but not nutritive, induced sleep in starved flies, but sleep bout length and the arousal threshold was short and decreased, respectively. On the other hand, sorbitol, which is not sweet but nutritive, did not induce sleep, but slightly increased the lowered arousal threshold. Activation of sweetness receptor expressing neurons induced sleep in starved flies. These results suggest that sweetness alone is sufficient to induce sleep in starved flies and that the nutritional status affects sleep homeostasis by decreasing the arousal threshold, which resulted in short sleep bouts in Drosophila.
India Is Overtaking China as the World’s Largest Emitter of Anthropogenic Sulfur Dioxide
Authors: Li Can , McLinden Chris , Fioletov Vitali , Krotkov Nickolay , Carn Simon , Joiner Joanna , Streets David , He Hao , Ren Xinrong , Li Zhanqing , Dickerson Russell R.
Published: October 2017

Severe haze is a major public health concern in China and India. Both countries rely heavily on coal for energy, and sulfur dioxide (SO2) emitted from coal-fired power plants and industry is a major pollutant contributing to their air quality problems. Timely, accurate information on SO2 sources is a required input to air quality models for pollution prediction and mitigation. However, such information has been difficult to obtain for these two countries, as fast-paced changes in economy and environmental regulations have often led to unforeseen emission changes. Here we use satellite observations to show that China and India are on opposite trajectories for sulfurous pollution. Since 2007, emissions in China have declined by 75% while those in India have increased by 50%. With these changes, India is now surpassing China as the world’s largest emitter of anthropogenic SO2. This finding, not predicted by emission scenarios, suggests effective SO2 control in China and lack thereof in India. Despite this, haze remains severe in China, indicating the importance of reducing emissions of other pollutants. In India, ~33 million people now live in areas with substantial SO2 pollution. Continued growth in emissions will adversely affect more people and further exacerbate morbidity and mortality.
Post-drought hydraulic recovery is accompanied by non-structural carbohydrate depletion in the stem wood of Norway spruce saplings
Authors: Tomasella Martina , Häberle Karl-Heinz , Nardini Andrea , Hesse Benjamin , Machlet Anna , Matyssek Rainer
Published: October 2017

Hydraulic failure and carbon starvation are recognized as main causes of drought-induced forest decline. As water transport and carbon dynamics are strictly interdependent, it is necessary to clarify how dehydration-rehydration cycles are affecting the relations between stem embolism and non-structural carbohydrates (NSC). This is particularly needed for conifers whose embolism repair capability is still controversial. Potted Norway spruce saplings underwent two drought-re-irrigation cycles of same intensity, but performed in two consecutive summers. During the second cycle, stem percent loss of hydraulic conductivity (PLC) and NSC content showed no carry-over effects from the previous drought, indicating complete long-term recovery. The second drought treatment induced moderate PLC (20%) and did not affect total NSCs content, while starch was converted to soluble sugars in the bark. After one week of re-irrigation, PLC recovered to pre-stress values (0%) and NSCs were depleted, only in the wood, by about 30%. Our data suggest that spruce can repair xylem embolism and that, when water is newly available, NSCs stored in xylem parenchyma can be mobilized over short term to sustain respiration and/or for processes involved in xylem transport restoration. This, however, might imply dependency on sapwood NSC reserves for survival, especially if frequent drought spells occur.
KRAS mutation and primary tumor location do not affect efficacy of bevacizumab-containing chemotherapy in stagae IV colorectal cancer patients
Authors: Sun De-cong , Shi Yan , Wang Yan-rong , Lv Yao , Yan Huan , Mao Hui , Wang Zhi-kuan , Wu Zhi-yong , Shi Wei-wei , Dai Guang-hai
Published: October 2017

This study aims to investigate the efficacy of bevacizumab-combined chemotherapy (BCC) in Chinese stage IV colorectal cancer (CRC), and analyze the relationship between clinicopathological features with survival. Patients with stage IV CRC treated with BCC were analyzed retrospectively. 217 metastatic CRC (mCRC) patients were collected, out of which79 were right-sided CRCs and 138 were left-sided ones. Patients with Eastern Cooperative Oncology Group (ECOG) performance status ≤2, single agent chemotherapy, poor/mucous/signet ring cell component, second-and further-line of bevacizumab administration, multiple metastasis sites had comparatively worse survival. Among 141 patients with known KRAS status, 55 patients harbored KRAS mutation and 86 had wild type KRAS. The ORR and DCR were 41.9% and 78.9%, respectively, in patients with wild type KRAS, while ORR and DCR was 38.7% and 77.9%, respectively, in patients with KRAS mutation. The median PFS of patients with wild type and mutant KRAS were 8.38, and9.59 months, respectively; whereas the OS was 23.00 and 21.26 months, respectively for mCRC patients with wild-type and mutant KRAS. Cumulatively, our study indicated that BCC was effective and beneficial for Chinese stage IV CRC patients. KRAS mutation status and tumor location were not a prognostic factor for survival.
Descendants of the Jurassic turiasaurs from Iberia found refuge in the Early Cretaceous of western USA
Authors: Royo-Torres Rafael , Upchurch Paul , Kirkland James I. , DeBlieux Donald D. , Foster John R. , Cobos Alberto , Alcalá Luis
Published: October 2017

A new, largely complete eusauropod dinosaur with cranial and postcranial elements from two skeletons, Mierasaurus bobyoungi gen. nov., sp. nov. from the lower Yellow Cat Member (Early Cretaceous) of Utah (USA), is the first recognized member of Turiasauria from North America. Moreover, according to our phylogenetic results, Moabosaurus utahensis from the lower Yellow Cat Member of Utah (USA) is also a member of this clade. This group of non-neosauropod eusauropods, which now includes five genera (Losillasaurus, Turiasaurus, Mierasaurus, Moabosaurus and Zby), was previously known only from the Jurassic of Europe. These recent discoveries in Utah suggest that turiasaurs as a lineage survived the Jurassic-Cretaceous extinction boundary and expanded their known range, at least, into western North America. The revised spatiotemporal distribution of turiasaurs is consistent with the presence of a land connection between North America and Europe sometime during the late Tithonian to Valanginian (c.147-133 Ma). Mierasaurus and Moabosaurus are the only non-neosauropod eusauropods known from North America, despite being younger than the classic neosauropods of the Morrison Formation (c.150 Ma).
Cereal products derived from wheat, sorghum, rice and oats alter the infant gut microbiota in vitro
Authors: Gamage Hasinika K. A. H. , Tetu Sasha G. , Chong Raymond W. W. , Ashton John , Packer Nicolle H. , Paulsen Ian T.
Published: October 2017

The introduction of different nutrient and energy sources during weaning leads to significant changes in the infant gut microbiota. We used an in vitro infant digestive and gut microbiota model system to investigate the effect of four commercially available cereal products based on either wheat, sorghum, rice or oats, on the gut microbiota of six infants. Our results indicated cereal additions induced numerous changes in the gut microbiota composition. The relative abundance of bacterial families associated with fibre degradation, Bacteroidaceae, Bifidobacteriaceae, Lactobacillaceae, Prevotellaceae, Ruminococcaceae and Veillonellaceae increased, whilst the abundance of Enterobacteriaceae decreased with cereal additions. Corresponding changes in the production of SCFAs showed higher concentrations of acetate following all cereal additions, whilst, propionate and butyrate varied between specific cereal additions. These cereal-specific variations in the concentrations of SCFAs showed a moderate correlation with the relative abundance of potential SCFA-producing bacterial families. Overall, our results demonstrated clear shifts in the abundance of bacterial groups associated with weaning and an increase in the production of SCFAs following cereal additions.
Biomechanical, ultrastructural, and electrophysiological characterization of the non-human primate experimental glaucoma model
Authors: Raghunathan VijayKrishna , Eaton J. Seth , Christian Brian J. , Morgan Joshua T. , Ver Hoeve James N. , Yang Chen-Yuan Charlie , Gong Haiyan , Rasmussen Carol A. , Miller Paul E. , Russell Paul , Nork T. Michael , Murphy Christopher J.
Published: October 2017

Laser-induced experimental glaucoma (ExGl) in non-human primates (NHPs) is a common animal model for ocular drug development. While many features of human hypertensive glaucoma are replicated in this model, structural and functional changes in the unlasered portions of trabecular meshwork (TM) of laser-treated primate eyes are understudied. We studied NHPs with ExGl of several years duration. As expected, ExGl eyes exhibited selective reductions of the retinal nerve fiber layer that correlate with electrophysiologic measures documenting a link between morphologic and elctrophysiologic endpoints. Softening of unlasered TM in ExGl eyes compared to untreated controls was observed. The degree of TM softening was consistent, regardless of pre-mortem clinical findings including severity of IOP elevation, retinal nerve fiber layer thinning, or electrodiagnostic findings. Importantly, this softening is contrary to TM stiffening reported in glaucomatous human eyes. Furthermore, microscopic analysis of unlasered TM from eyes with ExGl demonstrated TM thinning with collapse of Schlemm’s canal; and proteomic analysis confirmed downregulation of metabolic and structural proteins. These data demonstrate unexpected and compensatory changes involving the TM in the NHP model of ExGl. The data suggest that compensatory mechanisms exist in normal animals and respond to elevated IOP through softening of the meshwork to increase outflow.
Bioinspired Cell-Derived Nanovesicles versus Exosomes as Drug Delivery Systems: a Cost-Effective Alternative
Authors: Goh Wei Jiang , Zou Shui , Ong Wei Yi , Torta Federico , Alexandra Alvarez Fernandez , Schiffelers Raymond M. , Storm Gert , Wang Jiong-Wei , Czarny Bertrand , Pastorin Giorgia
Published: October 2017

Cell Derived Nanovesicles (CDNs) have been developed from the rapidly expanding field of exosomes, representing a class of bioinspired Drug Delivery Systems (DDS). However, translation to clinical applications is limited by the low yield and multi-step approach in isolating naturally secreted exosomes. Here, we show the first demonstration of a simple and rapid production method of CDNs using spin cups via a cell shearing approach, which offers clear advantages in terms of yield and cost-effectiveness over both traditional exosomes isolation, and also existing CDNs fabrication techniques. The CDNs obtained were of a higher protein yield and showed similarities in terms of physical characterization, protein and lipid analysis to both exosomes and CDNs previously reported in the literature. In addition, we investigated the mechanisms of cellular uptake of CDNs in vitro and their biodistribution in an in vivo mouse tumour model. Colocalization of the CDNs at the tumour site in a cancer mouse model was demonstrated, highlighting the potential for CDNs as anti-cancer strategy. Taken together, the results suggest that CDNs could provide a cost-effective alternative to exosomes as an ideal drug nanocarrier.
The necessity to choose causes reward-related anticipatory biasing: Parieto-occipital alpha-band oscillations reveal suppression of low-value targets
Authors: Heuer Anna , Wolf Christian , Schütz Alexander C. , Schubö Anna
Published: October 2017

Positive outcome of actions can be maximized by choosing the option with the highest reward. For saccades, it has recently been suggested that the necessity to choose is, in fact, an important factor mediating reward effects: latencies to single low-reward targets increased with an increasing proportion of interleaved choice-trials, in which participants were free to choose between two targets to obtain either a high or low reward. Here, we replicate this finding for manual responses, demonstrating that this effect of choice is a more general, effector-independent phenomenon. Oscillatory activity in the alpha and beta band in the preparatory period preceding target onset was analysed for a parieto-occipital and a centrolateral region of interest to identify an anticipatory neural biasing mechanism related to visuospatial attention or motor preparation. When the proportion of interleaved choices was high, an increase in lateralized posterior alpha power indicated that the hemifield associated with a low reward was suppressed in preparation for reward-maximizing target selection. The larger the individual increase in lateralized alpha power, the slower the reaction times to low-reward targets. At a broader level, these findings support the notion that reward only affects responses when behaviour can be optimized to maximize positive outcome.
A Fully-Automatic Multiparametric Radiomics Model: Towards Reproducible and Prognostic Imaging Signature for Prediction of Overall Survival in Glioblastoma Multiforme
Authors: Li Qihua , Bai Hongmin , Chen Yinsheng , Sun Qiuchang , Liu Lei , Zhou Sijie , Wang Guoliang , Liang Chaofeng , Li Zhi-Cheng
Published: October 2017

In fully-automatic radiomics model for predicting overall survival (OS) of glioblastoma multiforme (GBM) patients, the effect of image standardization parameters such as voxel size, quantization method and gray level on model reproducibility and prognostic performance are still unclear. In this study, 45792 multiregional radiomics features were automatically extracted from multi-modality MR images with different voxel sizes, quantization methods, and gray levels. The feature reproducibility and prognostic performance were assessed. Multiparametric and fixed-parameter radiomics signatures were constructed based on a training cohort (60 patients). In an independent validation cohort (32 patients), the multiparametric signature achieved better performance for OS prediction (C-Index = 0.705, 95% CI: 0.672, 0.738) and significant stratification of patients into high- and low-risk groups (P = 0.0040, HR = 3.29, 95% CI: 1.40, 7.70), which outperformed the fixed-parameter signatures and conventional factors such as age, Karnofsky Performance Score and tumor volume. This study demonstrated that voxel size, quantization method and gray level had influence on reproducibility and prognosis of radiomics features for GBM OS prediction. An automatic method to determine the optimal parameter settings was provided. It indicated that multiparametric radiomics signature had the potential of offering better prognostic performance than fixed-parameter signatures.
A generic phase between disordered Weyl semimetal and diffusive metal
Authors: Su Ying , Wang X. S. , Wang X. R.
Published: October 2017

Quantum phase transitions of three-dimensional (3D) Weyl semimetals (WSMs) subject to uncorrelated on-site disorder are investigated through quantum conductance calculations and finite-size scaling of localization length. Contrary to previous claims that a direct transition from a WSM to a diffusive metal (DM) occurs, an intermediate phase of Chern insulator (CI) between the two distinct metallic phases should exist due to internode scattering that is comparable to intranode scattering. The critical exponent of localization length is ν$$\,\simeq $$

≃
 1.3 for both the WSM-CI and CI-DM transitions, in the same universality class of 3D Gaussian unitary ensemble of the Anderson localization transition. The CI phase is confirmed by quantized nonzero Hall conductances in the bulk insulating phase established by localization length calculations. The disorder-induced various plateau-plateau transitions in both the WSM and CI phases are observed and explained by the self-consistent Born approximation. Furthermore, we clarify that the occurrence of zero density of states at Weyl nodes is not a good criterion for the disordered WSM, and there is no fundamental principle to support the hypothesis of divergence of localization length at the WSM-DM transition.
Heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) function as endocytic receptors for an internalizing anti-nucleic acid antibody
Authors: Park Hyunjoon , Kim Minjae , Kim Hye-Jin , Lee Yeonjin , Seo Youngsil , Pham Chuong D. , Lee Joungmin , Byun Sung June , Kwon Myung-Hee
Published: October 2017

A subset of monoclonal anti-DNA autoantibodies enters a variety of living cells. Here, we aimed to identify the endocytic receptors recognized by an internalizing anti-nucleic acid autoantibody, the 3D8 single-chain variable fragment (scFv). We found that cell surface binding and internalization of 3D8 scFv were inhibited markedly in soluble heparan sulfate (HS)/chondroitin sulfate (CS)-deficient or -removed cells and in the presence of soluble HS and CS. 3D8 scFv colocalized intracellularly with either HS proteoglycans (HSPGs) or CSPGs in HeLa cells. 3D8 scFv was co-endocytosed and co-precipitated with representative individual HSPG and CSPG molecules: syndecan-2 (a transmembrane HSPG), glypican-3 (a glycosylphosphatidylinositol (GPI)-anchored HSPG); CD44 (a transmembrane CSPG); and brevican (a GPI-anchored CSPG). Collected data indicate that 3D8 scFv binds to the negatively charged sugar chains of both HSPGs and CSPGs and is then internalized along with these molecules, irrespective of how these proteoglycans are associated with the cell membrane. This is the first study to show that anti-DNA antibodies enter cells via both HSPGs and CSPGs simultaneously. The data may aid understanding of endocytic receptors that bind anti-DNA autoantibodies. The study also provides insight into potential cell membrane targets for macromolecular delivery.
Microparticles controllable accumulation, arrangement, and spatial shaping performed by tapered-fiber-based laser-induced convection flow
Authors: Zhang Yu , Lei Jiaojie , Zhang Yaxun , Liu Zhihai , Zhang Jianzhong , Yang Xinghua , Yang Jun , Yuan Libo
Published: October 2017

The ability to arrange cells and/or microparticles into the desired pattern is critical in biological, chemical, and metamaterial studies and other applications. Researchers have developed a variety of patterning techniques, which either have a limited capacity to simultaneously trap massive particles or lack the spatial resolution necessary to manipulate individual particle. Several approaches have been proposed that combine both high spatial selectivity and high throughput simultaneously. However, those methods are complex and difficult to fabricate. In this article, we propose and demonstrate a simple method that combines the laser-induced convection flow and fiber-based optical trapping methods to perform both regular and special spatial shaping arrangement. Essentially, we combine a light field with a large optical intensity gradient distribution and a thermal field with a large temperature gradient distribution to perform the microparticles shaping arrangement. The tapered-fiber-based laser-induced convection flow provides not only the batch manipulation of massive particles, but also the finer manipulation of special one or several particles, which break out the limit of single-fiber-based massive/individual particles photothermal manipulation. The combination technique allows for microparticles quick accumulation, single-layer and multilayer arrangement; special spatial shaping arrangement/adjustment, and microparticles sorting.
Nasopharyngeal metagenomic deep sequencing data, Lancaster, UK, 2014–2015
Authors: Atkinson Kate V. , Bishop Lisa A. , Rhodes Glenn , Salez Nicolas , McEwan Neil R. , Hegarty Matthew J. , Robey Julie , Harding Nicola , Wetherell Simon , Lauder Robert M. , Pickup Roger W. , Wilkinson Mark , Gatherer Derek
Published: October 2017

Nasopharyngeal swabs were taken from volunteers attending a general medical practice and a general hospital in Lancaster, UK, and at Lancaster University, in the winter of 2014–2015. 51 swabs were selected based on high RNA yield and allocated to deep sequencing pools as follows: patients with chronic obstructive pulmonary disease; asthmatics; adults with no respiratory symptoms; adults with feverish respiratory symptoms; adults with respiratory symptoms and presence of antibodies against influenza C; paediatric patients with respiratory symptoms (2 pools); adults with influenza C infection (2 pools), giving a total of 9 pools. Illumina sequencing was performed, with data yields per pool in the range of 345.6 megabases to 14 gigabases after removal of reads aligning to the human genome. The data were deposited in the Sequence Read Archive at NCBI, and constitute a resource for study of the viral, bacterial and fungal metagenome of the human nasopharynx in healthy and diseased states and comparison with other metagenomic studies on the human respiratory tract.