bluuebunny/crossref_metadata_2025 huggingface dataset:
The effect of coach-athlete relationship on athlete burnout among college soccer players: The mediating role of training satisfaction
Authors: Liu Jiahao , Li Jing
Published: February 2024

Purpose: Athlete burnout is an essential adverse factor that usually decreases athletes’ sports performance and reduces their passion for entire athletic career development. The present study examined the association of coach-athlete relationship, training satisfaction, and athlete burnout, and then explored the training satisfaction as a mediating role of the effect of coach-athlete relationship on athlete burnout among college soccer players.
Methods: Two hundred and eighteen college soccer players who came from seven Chinese higher education institutions participated in this study. The Coach-Athlete Relationship Scale, Training Satisfaction Scale, and Athlete Burnout Questionnaire were used to measure the basic information of participants respectively. Pearson’s product-moment correlation analysis, linear regression analysis, and bootstrap method were conducted to analyze the collected data set.
Results: The results showed that 1) there was a significant correlation relationship between coach-athlete relationship, training satisfaction, and athlete burnout. 2) emotion, behavior, and cognition in coach-athlete relationship significantly negatively affected emotional/ physical exhaustion and sport devaluation in athlete burnout; emotion and behavior in coach-athlete relationship significantly negatively affected reduced athletic accomplishment in athlete burnout; emotion and behavior in coach-athlete relationship significantly positively affected training satisfaction; training satisfaction significantly negatively affected emotional/ physical exhaustion, reduced athletic accomplishment, and sport devaluation in athlete burnout. 3) training satisfaction has a significantly mediating effect on the relationship between coach-athlete relationship and athlete burnout.
Conclusion: Emotion and behavior in coach-athlete relationship can not only directly negative impact on athlete burnout but can also negatively influence athlete burnout through the mediating effect of training satisfaction. Therefore. We suggest that to mitigate athlete burnout, coaches should ensure timely communication with collegiate athletes during training, and collegiate athletes should incorporate mental training techniques into their routines.
Molecular, physiological and functional features underlying antipsychotic medication use related cortical thinning
Authors: Tuominen Lauri , Armio Reetta-Liina , Hansen Justine , Walta Maija , Koutsouleris Nikolaos , Laurikainen Heikki , Salokangas Raimo , Misic Bratislav , Hietala Jarmo
Published: February 2024

Use of antipsychotic medication is related to thinning of the cerebral cortex, but the underlying mechanisms of this effect remain largely unknown. Here, we investigated potential mechanisms across multiple levels of description, from molecular and physiological factors to whole-brain functional patterns. We first analyzed a single site discovery sample of patients (N=131) with early psychosis for whom antipsychotic related cortical thinning was estimated based on lifetime exposure to antipsychotics. Findings were replicated using data from a large (N≥2168) ENIGMA meta-analysis. We discovered that antipsychotic related cortical thinning is associated with a number of neurotransmitter systems, most notably the serotonin system, physiological measures, and functional networks and neural oscillatory power distributions typical for regions subserving higher cognition. At the functional level, antipsychotic related cortical thinning affects regions involved in executive function and motivation, but not perception. These results show how molecular, physiological, and large-scale functional patterns underlie antipsychotic related cortical thinning.
Recurrent spontaneous pneumothorax in a patient Wilson disease who is on long term D-penicillamine. Case report
Authors: Gunathilaka Nilanthi , LMH Bandara , Handagala S , WNS Kularatne
Published: February 2024

Background
Recurrent spontaneous pneumothoraces (PSP) in association with liver disease is well known to occur with alpha 1 antitrypsin deficiency (AATD). Wilson disease (WD) association to recurrent PSP is not a known entity.
Case presentation
A 42-year-old nonsmoker man with a history of recurrent PSP admitted with large right pneumothorax. Following intercostal chest drain (ICD) patient’s breathing improved and the lung expanded. Despite that, he continued to have a small residual pneumothorax (< 2cm). Twelve years ago, he was diagnosed to have WD. Patient was on treatment with D-penicillamine. After 3 years to the diagnosis of WD, patient had developed left sided recurrent PSPs where he ended up with left-pleurectomy in 2015. For the current event, initially expectant management was done for residual pneumothorax. All the possible causes for PSP were excluded. On follow up, he continued to be symptomatic. Thoracic surgical referral was arranged for Video-Assisted Thoracoscopic Surgery (VATS) and redo-pleurectomy. Safety of future treatment with D-penicillamine is not concluded as there is no convincing evidence to prove it as a culprit agent for PSPs. Multidisciplinary discussion arranged and consideration of treatment with alternative copper chelating therapy was emphasized.
Conclusions
Though rare, it is important to observe for occurrence of PSP in WD patients. This case report will be an eye opening for association of lung disease and WD and related treatment.
Research on Aerodynamic Drag Reduction of EMU Tail Car Bogie on Combined Suction and Blowing
Authors: Cui Hongjiang , Tang Huaiyu , Guan Ying , Wu Di
Published: February 2024

The flow field structure in the bogie region has an important impact on the aerodynamic drag of the EMU. In order to meet the lower aerodynamic drag requirements for further speed increases of EMU trains, this paper adopts numerical simulation method to study the active control drag reduction technology of suction and blowing air combined with bogie. The results indicate that the setting of suction and blowing air holes at the front and rear end plates of the tail bogie has only a drag reduction effect on the pressure drag in the aerodynamic drag of the bogie and tail car. With the change of suction and blowing air speeds, the drag reduction rate of the tail car reaches the optimal value of 3.82% at 0.05U, and the drag reduction rate of the bogie reaches the optimal value of 4.61% at 0.2U. The study on the combined suction and blowing air drag reduction method of the bogie has important significance in breaking through the limitations of traditional bogie aerodynamic drag reduction.
Identification of methylation markers for age and Bovine Respiratory Disease in dairy cattle
Authors: Psifidi Androniki , Attree Elizabeth , Griffiths Bethany , Panchal Ketankumar , Xia Dong , Werling Dirk , Banos Georgios , Oikonomou George
Published: February 2024

Methylation profiles of animals is known to differ by age and disease status. Bovine respiratory disease (BRD), a complex infectious disease, primarily affects calves and has significant impact on animal welfare and the cattle industry, predominantly from production losses. BRD susceptibility is multifactorial, influenced by both environmental and genetic factors. We investigated the epigenetic profile of BRD susceptibility in calves and age-related methylation differences between healthy calves and adult dairy cows using Reduced Representation Bisulfite Sequencing (RRBS).
We identified 3,452 genes within differentially methylated regions between calves and adults. Functional analysis revealed enrichment of developmental pathways including cell fate commitment and tissue morphogenesis. Between healthy and BRD affected calves, 964 genes were identified within differentially methylated regions. Immune and vasculature regulatory pathways were enriched and key candidates in BRD susceptibility involved in complement cascade regulation, vasoconstriction and respiratory cilia structure and function were identified.
An One-Year Randomised Controlled Clinical Trial to Assess the Effects of Two Desensitising Agents on Reducing Post-operative Sensitivity After Composite Restorations
Authors: Abd-Elkader Nessrin M. , El-Mahy Waleed A. , Afifi Rania R.
Published: February 2024

Background: Roughly 30% of individuals encounter post-operative sensitivity Subsequent to the placement of resin composite restorations in their posterior teeth, as indicated by various clinical research studies. To alleviate post-operative sensitivity, one approach involves the desensitization of teeth through the occlusion of dentinal tubules using dental desensitizers, aimed at reducing sensitivity following composite restorations.
Methods: A total of 10 patients, all having Class I cavities, were prepared for this study. These patients had a vital pulp and fell within the age range of 18 to 45, maintaining good oral hygiene. Patients with abnormal habits, traumatic occlusion, and gingival disease were excluded from the study. One of three groups was randomly allocated to the participants: Group I received Hurriseal, Group II was treated with Gluma desensitizer, and Group III functioned as the control group. After the dental desensitizer surface treatment and the evaluation of post-operative sensitivity, the teeth were subsequently restored with composite material.
Results: At 1 week, Significant statistical differences among the three groups were observed (p-value = 0.006), with Gluma demonstrating the lowest Visual Analog Scale (VAS) score in comparison to Hurriseal and the Control group.
Nevertheless, no statistically significant differences were identified between the groups at all other timepoints (P value > 0.05). Post-hoc comparisons among the three study groups revealed significant differences between Hurriseal and Gluma, although there were no appreciable variations found between the Gluma and Control groups.
Moreover, significant differences in VAS scores within each group over time were detected (p value= 0.03, 0.001, and 0.01, for Hurriseal, Gluma and control groups, respectively. Statistically significant differences were observed mainly during the comparisons involving the 1-month timepoint in all three groups, suggesting that the most notable changes in pain levels occurred around this period within each group.
Conclusion: Gluma proved to be the most efficient in minimizing postoperative sensitivity after composite restoration, whereas Hurriseal exhibited the least effectiveness in this regard.
Trial registration: The research was formally recorded in the clinicaltrials.gov database.
(#NCT06113874):(02/11/2023).
ClinicalTrials.gov Identifier: NCT06113874
A new method for unmanned aerial vehicle path planning in complex environments
Authors: He Yong , Hou Ticheng , Wang Mingran
Published: February 2024

In response to challenges in unmanned aerial vehicle (UAV) path planning, such as low search efficiency, non-smooth paths, and the inability to adapt to unknown environments, this paper proposes a novel UAV path planning method integrating a dual-layer optimization of A* and the dynamic window approach(DWA). Firstly, neighbor node pruning rules are defined to optimize the node expansion process of the A* algorithm. The heuristic function of the A* algorithm is dynamically adjusted based on obstacle coverage, and key path nodes are extracted using the Bresenham algorithm. Secondly, a deviation sub-function is introduced to enhance local path planning coherence, and an adaptive weighting method for the evaluation function is designed. Lastly, the optimized key nodes are utilized as sub-goals for local path planning, integrating the dynamic window approach to enhance real-time obstacle avoidance and adaptability in unknown environments. Simulation results demonstrate that the proposed method significantly improves the efficiency, smoothness, and obstacle avoidance performance of mobile robot path planning, meeting the requirements of complex planning tasks more effectively.
The Role of Social Media in Monitoring and Predicting Influenza Cases in China: A Time Series Analysis
Authors: Fang Chuangsen , Chen Jialong , Zeng Kewei , Wang Hongjian , Lan Yajia , Li Yanming
Published: February 2024

Background
Influenza remains a global health challenge, necessitating robust surveillance systems for timely intervention. With the prevalence of social media, its potential in health surveillance needs exploration.
Methods
Using outpatient and emergency department data from Beijing Hospital spanning three years (2020 to 2022), and social media data from Chinese platforms, we analyzed trends in influenza-like illness (ILI) cases and related media activity. Descriptive analysis, lead-lag correlation, and causal inference through the PCMCI algorithm were applied. The media’s impact on ILI case trends was quantified using Ordinary Least Squares (OLS) regression.
Results
Weekly analysis showed ILI cases peak during winter, with a consistent increase over the study period. Media index analysis revealed exponential growth and peaks aligning with ILI increases. Lead-lag correlation suggested a one-week lag between media index and ILI cases. PCMCI confirmed a causal relationship, indicating that ILI cases were positively correlated with the media index with a one-week delay. Model comparisons highlighted the improved predictive capability when incorporating media index data.
Conclusion
The study confirmed the utility of social media data as an augmenting factor for influenza surveillance, providing vital insights into outbreak dynamics. The incorporation of media indices in predictive models could enhance early warning systems, offering a promising avenue for public health strategies in China and similar settings.
Establishment of an efficient regeneration system of ‘ZiKui’ tea with hypocotyl as explants
Authors: Jin Jiongyi , Chen Yulu , Cai Ju , Lv Litang , Zeng XiaoFang , Li Jianrong , Asghar Sumeera , Li Yan
Published: February 2024

Zikui (Camellia sinensis cv. Zikui) is a recently discovered cultivar of local purple tea in Guizhou, China and it is a perennial woody plant, mainly propagated by cuttings. It is also a model plant for the study of anthocyanins,but the limited germplasm resources and the limitation of traditional reproduction seriously hinder its application. Here, an efficient regeneration system is established by using hypocotyl as explants for the first time. Different plant growth regulators (PGRs) were evaluated during different regeneration processes including callus and root induction. According to our findings, using the optimal disinfection conditions, the embryo contamination rate was 17.58 %. Additionally, the mortality rate was 9.69 %, while the survival rate was measured as 72.73 %. Moreover, the highest germination rate of 93.64% was observed under MS+2.40 mg/L GA3 medium conditions. The optimal callus induction rate was 95.19 %, while the optimal adventitious bud differentiation rate was 20.74%, Medium with 1.6mg L−1 IBA achieved 68.6% rooting of the adventitious shoots.The survival rate was more than 65% after 6 days growth in the cultivated matrix. In summary, our research aims to establish a regeneration system for Zikui tea plants and design a transformation system for tea plant tissue seedlings. This will enable the transfer of the target gene and ultimately facilitate the cultivation of new tea varieties with unique characteristics.
Using the Health Belief Model to Assess the Impact of Latent Tuberculosis Infection Health Education Video Towards Screening Adoption in Foreign-Born Persons Living in California
Authors: Ojukwu Juliana Uzoma , Stimatze Tamara
Published: February 2024

Background
Tuberculosis (TB) disproportionately affects foreign-born persons from TB-endemic countries. Previous studies demonstrated that educational interventions effectively increased knowledge, perception, and latent tuberculosis infection (LTBI) screening in at-risk people. Given the high prevalence of LTBI and active TB cases and the large proportion of foreign-born individuals with low LTBI awareness residing in California, this study sought to evaluate the impact of video-based LTBI education in this population.
Methods
We evaluated the impact of a 5-minute LTBI educational video on participants using Health Belief Model (HBM) constructs using a pre- and post-test design. We enrolled 84 participants during the study period. Participants identified as (54%) women and 45% men, with 54.48 mean age, and participants identified as Asian (48%), White (37%), Hispanic/Latinx (13%), and Black/African American or Native Hawaiian/Pacific Islander (2%). Participants first completed the pre-survey, which consisted of the HBM LTBI Survey, followed by a demographic survey. Participants then watched the educational intervention video followed by the post-survey, which consisted of the HBM LTBI Survey.
Results
To assess the changes in HBM constructs, we ran six paired-sample t-tests and found a significant increase in perceived susceptibility, t(83) = 8.82, p < .001, perceived severity, t(83) = 2.06, p < .04, perceived benefits, t(83) = 3.33, p < .001 and behavioral intention, t(82) = 3.99, p < .001 with a significant decrease in perceived barriers, t(83) = -3.38, p < .001. To analyze the impact of the HBM constructs on behavioral intentions, we ran a multiple linear regression. Overall, the HBM accounted significantly in variance for behavioral intentions to engage in screening, F(5, 77) = 14.81, p < 0.001; with perceived susceptibility, t(81) = 2.64, p = 0.01, perceived severity t(81) = 2.69, p = 0.009, and self-efficacy t(81) = 3.05, p = 0.003 significantly predicting behavioral intentions for LTBI screening.
Conclusions
This project demonstrates the efficacy of health education videos in promoting awareness and screening for LTBI. The authors recommend using health educational videos in communities and healthcare facilities to create more knowledge, awareness, and engagement in LTBI screening.
In-Vivo Assessment of Movement-Evoked Pain using Digital Technology in Patients with Chronic Back Pain: Implications for treatment personalization
Authors: Frumkin Madelyn , Greenberg Jacob , Zhang Justin , Javeed Saad , Zhang Jingwen , Xu Ziqi , Benedict Braeden , Botterbush Kathleen , Ray Wilson , Lu Chenyang , Rodebaugh Thomas
Published: February 2024

Digital methods are increasingly used to monitor activity and pain, yet few studies combine data streams to assess clinically relevant constructs, such as movement-evoked pain (MEP). In this study of patients with chronic back pain being evaluated for spine surgery, in-vivo assessment of MEP was uncorrelated with pre-surgical pain, interference, or physical function. In support of our hypothesis, however, patients exhibiting greater preoperative MEP demonstrated greater improvement in pain interference one month after spine surgery. Digital technology can be leveraged to improve treatment recommendations for patients with chronic pain.
Prospective Study of Sars-cov2 Associated Coagulopathy and Role of Complement Activation
Authors: Silingardi Mauro , Zappulo Fulvia , Dormi Ada , Pizzini Attilia Maria , Donadei Chiara , Fantoni Chiara , Zaccaroni Stefania , Pizzuti Valeria , Cilloni Nicola , Tantillo Simona , Guidi Antonella , Mancini Rita , Manna Gaetano La , Comai Giorgia
Published: February 2024

Sars-CoV2 associated coagulopathy is a complex entity. Platelets, coagulation factors, fibrinolysis, inflammatory cytokines, immunothrombosis, antiphospholipd antibodies, von Willebrand factor/ADAMTS13 axis, complement system have all been demonstrated to be actively involved in the determination of thrombotic events. Til now retrospective studies have analyzed the activaction of vWF/ADAMTS13 axis and complement involvement. We performed a prospective study with the aim of describing clinical and laboratoristic features of Sars-CoV2 associated coagulopathy and its relationship with complement activation. Biochemical variables, vWF/ADAMTS13 axis, complement factors of the enrolled patients have been analyzed. These variables have been correlated to clinical outcome of the disease. Covid associated coagulopathy is neither a Trombotic Trombocitopenc Purpura (TTP) nor and atypical hemolytic uremic syndrome (aSEU). Nevertheless, imbalance of vWF/Adamts13 axis and complement activation simultaneously occurre and are significantly higher in the severe form of disease.
Degradation of Reactive Red (B-3BF) Dye Wastewater Using UV Irradiation (254/185nm) with Sodium Persulfate in a Pilot UV Device
Authors: Wang Chao , Li Yongqiang , Wan Junmin , Hu Yi , Huang Yi , Qiu Jiangen
Published: February 2024

Two low-pressure ultraviolet (UV) lamps at 185/254 nm with sodium persulfate in a pilot UV device were utilized for the degradation of reactive red (B-3BF) dye wastewater compared with two UV lamps at 185/185 nm and two UV lamps at 254/254 nm. The degradation performances of UV irradiation (254/185 nm) with sodium persulfate under different degradation times, flow rates, initial pH, initial Na2S2O8 concentrations and initial dye concentrations were investigated. The experimental results illustrated that the degradation percentage of B-3BF dye could reduce to 90.42% with the energy consumption of 85.1 kWh/kg and the residual dye concentration of 1.92 mg/L by UV irradiation (254/185 nm) with initial Na2S2O8 concentration of 1.5 mmol/L and initial dye concentration of 20 mg/L. In addition, degradation performance of B-3BF dye wastewater by UV irradiation (254/185 nm) with sodium persulfate was more effective than those of UV irradiation (254/254 nm) and UV irradiation (185/185 nm). Therefore UV irradiation (254/185 nm) with sodium persulfate was promising for the degradation of B-3BF dye wastewater.
Single-cell RNA sequencing has unveiled the contribution of ADSCs in reprogramming metabolism in triple-negative breast cancer
Authors: Zhang Lingling , Xiao Qinyu , Zhang Mili , Liu Xiaojie , Zhou Lijie
Published: February 2024

Background:
Mesenchymal stem cells (MSCs) constitute a significant element of the tumor microenvironment (TME), exerting dynamic influences on various tumors through mechanisms such as chemotaxis, interaction with immune cells. Therefore, MSCs have the ability to dynamically affect various types of tumors. The purpose of this study was to determine the impact of three common MSCs on tumors using single-cell analysis technology.
Methods:
We accessed single-cell sequencing date for mesenchymal stromal stem cells from adipose tissue (ADSCs), human bone marrow (BMSCs), and stem cells from human exfoliated deciduous teeth (SHED) from GEO database (GSE157863). We utilized R packages, including Seurat and Rtsne, for analyzing difference between ADSCs and other MSCs. Subsequently, ADSCs were injected into nude mice bearing mammary tumor cells, and PET-CT was conducted to compare metabolic absorption rates before and after injection. Additionally, bioinformatic analysis and Immunohistochemistry (IHC) were utilized to investigate the function and distribution of CCDC80.
Results:
Bioinformatic analysis revealed that ADSCs differed from BMSCs and SHED, suggesting a potential inhibition of tumor growth through alterations in glycine metabolism. In vivo experiments using a triple-negative breast cancer model demonstrated that the injection of ADSCs inhibited tumor growth. Furthermore, our findings implicated CCDC80 as a potentially pivotal gene influencing metabolism.
Conclusion:
The data derived from this study indicate that ADSCs possess unique capabilities to modulate metabolism and suppress tumor growth. Consequently, ADSCs may emerge as a promising cellular treatment option for triple-negative breast cancer, a subtype currently lacking effective therapeutic interventions.
Association between Systemic Immune-Inflammation Index and stress urinary incontinence in adult women: a population-based study
Authors: Jiang Mei , Yao Xiaodie , Xu Pengfei , Jiang Hua
Published: February 2024

Background
Systemic immune-inflammation index (SII) is a novel inflammatory marker, and inflammation may contribute significantly to the aetiology of stress urinary incontinence (SUI), in addition to recognised factors. The aim of this study was to explore the associations between SII and SUI in women.
Methods
Adult participants (≥ 20 years) from the 2007–2018 NHANES were included. The SII level was calculated as the platelet count×neutrophil count/lymphocyte count. Weighted multivariate logistic regression and subgroup analysis were conducted to determine the relationship between SII and SUI in women. The non-linear relationship between SII and SUI risk was evaluated using restricted cubic splines (RCS), and the inflection point was determined by two-piecewise logistic regression.
Results
A total of 10,776 adult women were included, of whom 4407 (40.9%) had SUI. After adjusting for all confounders, a significant positive association between SII and SUI risk was observed (OR: 1.09; 95%CI: 1.01–1.19, P = 0.021). Moreover, when compared with the patients in the lowest SII tertile, those in the highest SII tertile had a 15% increased risk of SUI (OR: 1.15; 95%CI: 1.00-1.31, P = 0.049) after adjustment for all confounders. Subgroup analysis showed that there were consistent relationships between SII level and SUI across most subgroups. A non-linear relationship between log2SII and SUI was observed by RCS analysis. Furthermore, the two-piecewise logistic regression demonstrated that the odds of being SUI increased with the SII level, and this rising trend gradually slowed down after passing the inflection point of 8.64.
Conclusion
Our findings. suggest an association between elevated SII levels and an increased likelihood of SUI in women. Further well-designed prospective studies are needed to substantiate our results
Xiashengella succiniciproducens gen. nov., sp. nov., a succinate-producing bacterium isolated from an anaerobic digestion tank in the family Marinilabiliaceae of the order Bacteroidales
Authors: Huang Hao-Jie , Zhang Xi , Sun Xin-Wei , Chen Biao , Li Xiu-Tong , Zhou Nan , Abdugheni Rashidin , Cheng Qiu-Yin , Zhang Tie-Jun , Liu Yao , Jiang Yong , Deng Ye , Liu Shuang-Jiang , Jiang Cheng-Ying
Published: February 2024

A strictly anaerobic, motile bacterium, designated as strain Ai-910T, was isolated from the sludge of an anaerobic digestion tank in China. Cells were Gram-stain-negative rods. Optimal growth was observed at 38°C (growth range 25–42°C), pH 8.5 (growth range 5.5–10.5), and under a NaCl concentration of 0.06% (w/v) (range 0–2.0%). Major cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The respiratory quinone was MK-7. Using xylose as the growth substrate, succinate was produced as the fermentation product. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain Ai-910T formed a distinct phylogenetic lineage that reflects a new genus in the family Marinilabiliaceae, sharing high similarities to Alkaliflexus imshenetskii Z-7010T (92.78%), Alkalitalea saponilacus SC/BZ-SP2T (92.51%), and Geofilum rubicundum JAM-BA0501T (92.36%). Genomic similarity (average nucleotide identity and digital DNA-DNA hybridization) values between strain Ai-910T and its phylogenetic neighbors were below 65.27 and 16.90%, respectively, indicating that strain Ai-910T represented a novel species. The average amino acid identity between strain Ai-910T and other related members of the family Marinilabiliaceae were below 69.41%, supporting that strain Ai-910T was a member of a new genus within the family Marinilabiliaceae. Phylogenetic, genomic, and phenotypic analysis revealed that strain Ai-910T was distinguished from other phylogenetic relatives within the family Marinilabiliaceae. The genome size was 3.10 Mbp, and the DNA G + C content of the isolate was 42.8 mol%. Collectively, differences of the phenotypic and phylogenetic features of strain Ai-910T from its close relatives suggest that strain Ai-910T represented a novel species in a new genus of the family Marinilabiliaceae, for which the name Xiashengella succiniciproducens gen. nov., sp. nov. was proposed. The type strain of Xiashengella succiniciproducens is Ai-910T (= CGMCC 1.17893T = KCTC 25304T).
Enhanced Methods for Evaluating Aquifer Susceptibility: Incorporating Static and Dynamic Vulnerability Assessments.
Authors: Ourarhi Sofia , Barkaoui Alae-Eddine , Zarhloule Yassine
Published: February 2024

This study transforms the concept of the intrinsic vulnerability of aquifers to pollution by integrating static and dynamic elements into the assessment approach. Our innovative methodology simplifies hydrogeological parameters, facilitating the assessment of groundwater aquifer vulnerability while enabling in-depth analysis of future scenarios, including t the impact of human activities and climate change on the hydrological cycle. The results of our analysis reveal that the dynamic vulnerability method (GCITF) and the conventional DRASTIC method generated 12.15% and 9.30% for the high vulnerability zone, respectively. In comparison, the SINTACS method estimated a low percentage of 0.28%. Overlaying the vulnerability maps revealed agreement between the GCITF, DRASTIC and SINTACS methods in the high-vulnerability zones. In particular, the GCITF method showed a more significant extension in zone 2, characterized by an annual extracted volume exceeding 40 Mm3/km2, underlining the importance of considering these sub-factors in dynamic vulnerability assessments. Bivariate statistical analysis, in particular Pearson correlation, revealed a moderate and statistically significant positive association between dynamic vulnerability on the one hand and DRASTIC and SINTACS methods on the other. These results underline the importance of integrating dynamic sub-factors for a more accurate and comprehensive vulnerability assessment, reflecting the complexity of hydrological and anthropogenic interactions influencing aquifer vulnerability.
Application of integrated nursing in minimally invasive rotatory resection for multiple breast tumors.
Authors: Chen Xiaohui , gong yanyan
Published: February 2024

Objective/Purpose:
The objective of this study is to investigate the application of integrated nursing in the minimally invasive rotatory resection for multiple breast tumors.
Method
A multicenter, prospective research strategy was adopted to recruit a specific number of patients who underwent minimally invasive rotatory resection for multiple breast tumors. This approach involved providing systematic and individualized treatment to the patients.
Results
The application of integrated nursing measures has resulted in reducing surgery time, enhancement of surgical results, mitigation of surgical complications, and facilitation of patient recovery. The incidence of postoperative hemorrhage and infection is decreased. Furthermore, research has demonstrated that integrated nursing can enhance patients' psychological well-being and elevate their satisfaction and overall quality of life [1].
Conclusion
The results of this study suggest that integrated nursing has wide-ranging potential in the use of minimally invasive rotatory resection for multiple breast tumors, and it can offer excellent nursing solutions for minimally invasive clinical breast surgery.
Genome-wide characterization of BnaLEA gene family reveals a positive role of BnaLEA39 in freezing tolerance in rapeseed (Brassica napus L.)
Authors: Wang Weiping , Liu Yan , Kang Yu , Liu Wei , Li Shun , Wang Zhonghua , Xia Xiaoyan , Chen Xiaoyu , Qian Lunwen , Xiong Xinghua , Liu Zhongsong , Guan Chunyun , He Xin
Published: February 2024

Background
Freezing stress is one of the major abiotic stresses that causes extensive damage to plants. LEA (Late embryogenesis abundant) proteins play a crucial role in plant growth, development, and abiotic stress. However, there is limited research on the function of LEA genes in low-temperature stress in Brassica napus (rapeseed).
Results
Total 148 potential LEA genes were identified in B. napus and divided into eight subgroups. BnaLEA genes of the same subgroup had similar gene structures and predicted subcellular locations. Cis-regulatory elements analysis showed that the promoters of BnaLEA genes rich in cis-regulatory elements related to various abiotic stresses. Additionally, RNA-seq and real-time PCR results indicated that the majority of BnaLEA family members were highly expressed in senescent tissues of rapeseed, especially during late stages of seed maturation, and most BnaLEA genes can be induced by salt and osmotic stress. Interestingly, the BnaLEA39 and BnaLEA40 genes were highly expressed across different vegetative and reproductive organs during different development stages, and showed strong responses to salt, osmotic, and cold stress, particularly freezing stress. Further analysis showed that overexpression of BnaLEA39 increased the freezing tolerance in rapeseed, as evidenced by lower relative electrical leakage and higher survival rates compared to the wild-type (WT) under freezing treatment.
Conclusion
This study is of great significance for understanding the functions of BnaLEA genes in freezing tolerance in rapeseed and offers an ideal candidate gene (BnaLEA39) for molecular breeding of freezing-tolerant rapeseed cultivars.
Quercetin promotes the proliferation and maturation of NK cells by binding to MYH9 and improves cognitive functions in aged mice
Authors: Su Tingting , Shen Haitao , He Mengyuan , Yang Shanshan , Gong Xue , Huang Ce , Guo Liuling , Wang Hao , Feng Shengyu , Mi Taotao , Zhao Meili , Liu Qing , Huo Fenjiao , Zhu Jian-Kang , Zhu Jianbo , Li Hongbin , Liu Hailiang
Published: February 2024

Background: Quercetin is a flavonol compound widely distributed in plants that possesses diverse biological properties, including antioxidative, anti-inflammatory, anticancer, neuroprotective and senescent cell-clearing activities. It has been shown to effectively alleviate neurodegenerative diseases and enhance cognitive functions in various models. The immune system has been implicated in the regulation of brain function and cognitive abilities. However, it remains unclear whether quercetin enhances cognitive functions by interacting with the immune system.
Results: In this study, middle-aged female mice were administered quercetin via tail vein injection. Quercetin increased the proportion of NK cells, without affecting T or B cells, and improved cognitive performance. Depletion of NK cells significantly reduces cognitive ability in mice. RNA-seq analysis revealed that quercetin modulated the RNA profile of hippocampal tissues in aging animals towards a more youthful state. In vitro, quercetin significantly inhibited the differentiation of Lin−CD117+ hematopoietic stem cells into NK cells. Furthermore, quercetin promoted the proliferation and maturation of NK cells by binding to the MYH9 protein.
Conclusions: In summary, our findings suggest that quercetin promotes the proliferation and maturation of NK cells by binding to the MYH9 protein, thereby improving cognitive performance in middle-aged mice.
Nest excavators’ learning walks in the Australian desert ant Melophorus bagoti
Authors: Deeti Sudhakar , McLean Donald James , Cheng Ken
Published: February 2024

The Australian red honey ant, Melophorus bagoti, stands out as the most thermophilic ant in Australia, engaging in all outdoor activities during the hottest periods of the day during summer months. This species of desert ants often navigates by means of path integration and learning landmark cues around the nest. In our study, we observed the outdoor activities of M. bagoti workers engaged in nest excavation, the maintenance of the nest structure, primarily by taking excess sand out of the nest. Before undertaking nest excavation, the ants conducted a single exploratory walk. Following their initial learning expedition, these ants then engaged in nest excavation activities. Consistent with previous findings on pre-foraging learning walks, after just one learning walk, the desert ants in our study demonstrated the ability to return home from locations 2 metres away from the nest, although not from locations 4 metres away. These findings indicate that even for activities like dumping excavated sand within a range of 5–10 cm outside the nest, these ants learn and utilize the visual landmark panorama around the nest.
Clinical and Biological Profile and Factors Associated With High Blood Lead Levels in Chronic Hemodialysis Patients in a Western French Guiana Hospital Center
Authors: BUNKETE Arriel MAKEMBI , FERMIGIER Florence , SIDIBE Modi , GONDELE David , ANGA Kazi , MPIBI Blady , EDJOKOLA Alphonse , BAFUNYEMBAKA Gabriel , KUAMBA Pascal , MORRY Franchisca-Anaïs , KASHALA Yannick , SIDIBE Mohamed , BELGRINE Malika , DAVODOUN Timote , DJICONKPODE Irenée , GBAGUIDI Tanguy
Published: February 2024

Background: Lead is toxic to the body. Its chronic intoxication combines various clinical and biological disorders that can be life-threatening. In French Guiana, lead poisoning is particularly worrying, as the incidence rate is nearly sixty times higher than in metropolitan France. In chronic hemodialysis patients, lead levels are often higher and can lead to several adverse consequences. Hence, the interest of this study, which is to describe the clinical and biological characteristics of chronic hemodialysis patients with high blood lead levels and to identify the associated factors to draw attention to its screening and the prevention of its complications.
Methods: Descriptive and analytical cross-sectional study that included 65 patients on chronic conventional hemodialysis: with an annual biological assessment in December 2022, including a serum lead assay. The outcome was the notion of hyper lead level, defined by a lead level > 85 μg/l. We described the clinical, biological, and dialytic parameters of patients with hyper lead levels and in logistic regression, we identified the factors that are correlated according to a significance threshold P<0.05.
Results: In all, 54% of patients had hyperplumbemia, 2/3 of them women. They were older, with an average age of 62. None of the patients had been occupationally exposed to lead. 94% were hypertensive and half were diabetic. 26% had anemia, and half had erythropoietin resistance. Their ferritin levels were slightly lower, with a mean of 721 μg/l. Mean albumin was 30 g/l, prealbumin 28 g/l, mean parathyroid hormone 1355 ng/ml, NT-pro BNP 9144 ng/ml. Mean CRP was 10.8 mg/l. They had collapsed residual diuresis and natriuresis with averages of 141 ml and 12 mmol/24 hours, respectively. There was a significant positive correlation between high BLL levels and young age, and a negative correlation with female gender, low serum albumin, prealbumin, protein and ferritin levels, as well as collapsed residual diuresis.
Conclusion: High blood lead levels are common in the Guyanese chronic hemodialysis population in which it is correlated with female sex, malnutrition, iron deficiency and residual poor renal function and probably with resistance to erythropoietin treatment. It is necessary to screen in at-risk populations to prevent complications associated with it.
A Strategy for Optimal and Selective Utilization of Multiple Interline DC Power Flow Controllers in VSC-HVDC Grids
Authors: Pourmirasghariyan Mirhamed , Yazdi Seyed Saeid Heidari , Milimonfared Jafar , Abbasipour Mehdi , Bagheri Mehdi
Published: February 2024

Inserting interline dc power flow controllers (IDC-PFCs) promote the control flexibility of the DC power flow (DC-PF) in meshed voltage source converter (VSC)-based high voltage direct current (HVDC) grids. Moreover, the steady-state security of the VSC-HVDC grids can be enhanced significantly when the IDC-PFCs are optimally placed and tuned. Therefore, it is proposed to apply minimum spanning tree (MST) graph theory to specify safe subgraph and unsafe subgraphs (which are prone to getting overloaded) of the VSC-HVDC grids and provide graphical insights on the static security conditions. The concept of the current variation index (CVI) is introduced to find the optimal locations of the IDC-PFCs within the identified unsafe subgraphs with maximized current alteration capability. The location with the highest value of CVI is considered the optimal placement scenario. As the focus would be on unsafe subgraphs, the optimal placement process would be done with the minimum computational burden. Then, static mixed-integer power injection models (MI-PIMs) are proposed to model multiple optimally-placed IDC-PFCs and develop mixed-integer security-constraint dc optimal power flow (MI-SC DC-OPF) formulations. The proposed MI-SC DC OPF would simultaneously activate the minimum required number of most effective optimally-placed IDC-PFCs and specify optimum references for them according to the operating point of the VSC-HVDC grid. The effective performances of the proposed placement strategy and selective/optimal operation method are evaluated on CIGRE B4 and IEEE 57-bus VSC-HVDC girds by performing static analysis.
Using tracer particle kinematics to predict particle size in rotating drums
Authors: Laudari Sudip , Marks Benjy , Rognon Pierre
Published: February 2024

Comminution is an energy intensive process. In ball mills, it is achieved by rotating a drum in which large metal balls crush ore particles. In-situ monitoring of particle size would be of considerable interest to optimize their operation. However, there is no established solution to measure particle size in such a harsh mechanical environment. We show here that the acceleration of the grinding media, which can be monitored using embedded accelerometers, can be used to sense the particle size during operation. In DEM simulations, we find that a machine learning classifier is able to detect the size of small "ore" particles solely based on the knowledge of the acceleration of larger grinding media particles. Results show that this kinematic sensing is effective over a wide range of particle size ratios, mixture ratio and mill charge. Beyond their practical interest in mineral processing, these results point out that the kinematics of large particles is affected by the size of the smaller particles, an observation which can help advance rheological models for bi-disperse granular flows.
Cadmium exposure causes mouse spermatogonia apoptosis via inducing endoplasmic reticulum stress
Authors: Wu Jie , Yin Qizi , Wang Yi , Wang Rong , Gong Wenjing , Chen Yihang , Zhang Mingming , Liu Yehao , Ji Yanli
Published: February 2024

Cadmium (Cd), which can cause testicular germ cell apoptosis, is a well-recognized male reproductive toxicant, but the underlying mechanism still needs investigation. To detect Cd toxicity on testicular germ cell, we treated mouse spermatogonia with CdCl2 in vitro and investigated the responses from cells at both RNA and protein levels. After treating mouse-derived spermatogonia cell line GC-1 spg cells with 20 µM CdCl2 for 24h, cell apoptosis was measured by TUNEL and flow cytometry assay. After then, the expressions of key genes and protein biomarkers involved in endoplasmic reticulum (ER) stress were detected by qPCR and western blot, respectively. Finally, untargeted metabolomics was performed to compare metabolic differences, and Illumina RNA sequencing was conducted to screen differentially expressed genes (DEGs). Our results indicated that Cd exposure caused cell apoptosis, DEGs were involved in several apoptosis-related pathways. Cd exposure apparently elevated the mRNA and protein expressions levels of both GRP78 and ATF6α, and disrupted the expression of many types of metabolites, especially for amino acids. Taken together, our study uncovers the pathway of Cd toxicity on mouse spermatogonia, provides deep understanding on Cd-induced testicular toxicity.
Lotus Elixir: A melody of gene expression variation in Oncorhynchus mykiss tissues
Authors: Maqsood Hakim Mudasir , Ganai Nazir Ahmad , Ahmad Syed Mudasir , Asimi Oyais Ahmad
Published: February 2024

The present study evaluated the survival and immune response of Rainbow trout (Oncorhynchus mykiss) fingerlings fed with Nelumbo nucifera leaf extract (NNLE) for 90-day time period. Two hundred and fifty fingerlings were randomly distributed into five treatment groups in triplicates. Each group was fed isonitrogenous and isoenergetic regimes containing 0% NNLE (control), 0.25% NNLE (T1), 0.75% NNLE (T2), 1.25% NNLE (T3), and 1.75% NNLE (T4) to satiation. NNLE was found to significantly (P < 0.05) reduce malate dehydrogenase and aspartate amino-transferase activity in the liver tissue. Alanine amino-transferase activity in the muscle tissue of NNLE fed groups was significantly higher than in the control. The liver and gill superoxide dismutase activity were significantly reduced in the NNLE fed groups compared to the control. Also, the catalase activity in the liver and gill got significantly lowered in the T4 group. NNLE fed groups showed elevated RBC, TLC, respiratory burst activity, lysozyme activity and phagocytic activity compared to control group. Also, mRNA expression of defensin and hepcidingenes showed significant upregulation in NNLE fed groups thereby highlighting its role in modulation of innate immune response. During pre-challenge phase, pro inflammatory cytokines were significantly (p<0.05) down-regulated compared to control. Whereas, post-challenge period, T4 (1.75% NNLE) caused substantial (p<0.05) up-regulation of IL-1 and TNF-α1 expression. Regulatory cytokines were slightly up-regulated during pre-challenge and down-regulated post-challenge periods. Treatment groups T3 and T4 showed significantly (p<0.05) higher survival rate after challenged with pathogenic bacteria Aeromonas hydrophila. The study highlights that supplementation of Indian lotus leaf extract at the rate of 1.75% in diet enhances the disease resistance in rainbow trout.
Doublecortin-like kinase is required for cnidocyte development in Nematostella vectensis
Authors: Kraus Johanna E. M. , Busengdal Henriette , Kraus Yulia , Rentzsch Fabian
Published: February 2024

The complex morphology of neurons requires precise control of their microtubule cytoskeleton. This is achieved by microtubule-associated proteins (MAPs) that regulate the assembly and stability of microtubules, and transport of molecules and vesicles along them. While many of these MAPs function in all cells, some are specifically or predominantly involved in regulating microtubules in neurons. Here we use the sea anemone Nematostella vectensis as a model organism to provide new insights into the early evolution of neural microtubule regulation. As a cnidarian, Nematostella belongs to an outgroup to all bilaterians and thus occupies an informative phylogenetic position for reconstructing the evolution of nervous system development. We identified an ortholog of the microtubule-binding protein doublecortin-like kinase (NvDclk1) as a gene that is predominantly expressed in neurons and cnidocytes (stinging cells), two classes of cells belonging to the neural lineage in cnidarians. A transgenic NvDclk1 reporter line revealed an elaborate network of neurite-like processes emerging from cnidocytes in the tentacles and the body column. A transgene expressing NvDclk1 under the control of the NvDclk1 promoter suggests that NvDclk1 is indeed a microtubule-binding protein. Further, we generated a mutant for NvDclk1 using CRISPR/Cas9 and show that the mutants fail to generate mature cnidocytes. Our results support the hypothesis that the elaboration of programs for microtubule regulation occurred early in the evolution of nervous systems.