bluuebunny/crossref_metadata_2025 huggingface dataset:
Cell cycle arrest and apoptosis are not dependent on p53 prior to p53-dependent embryonic stem cell differentiation
Authors: Jaiswal Sushil K. , Oh John J. , DePamphilis Melvin L.
Published: June 2020

Previous efforts to determine whether or not the transcription factor and tumor suppressor protein p53 is required for DNA damage-induced apoptosis in pluripotent embryonic stem cells (ESCs) produced contradictory conclusions. To resolve this issue, p53+/+ and p53−/− ESCs derived by two different methods were used to quantify time-dependent changes in nuclear DNA content; annexin-V binding; cell permeabilization; and protein expression, modification, and localization. The results revealed that doxorubicin (Adriamycin [ADR]) concentrations 10 to 40 times less than commonly used in previous studies induced the DNA damage-dependent G2-checkpoint and completed apoptosis within the same time frame, regardless of the presence or absence of p53, p21, and PUMA. Increased ADR concentrations delayed initiation of apoptosis in p53−/− ESCs, but the rates of apoptosis remained equivalent. Similar results were obtained by inducing apoptosis with either staurosporine inhibition of kinase activities or WX8 disruption of lysosome homeostasis. Differentiation of ESCs by LIF deprivation revealed p53-dependent formation of haploid cells, increased genomic stability, and suppression of the G2-checkpoint. Minimal induction of DNA damage now resulted in p53-facilitated apoptosis, but regulation of pluripotent gene expression remained p53-independent. Primary embryonic fibroblasts underwent p53-dependent total cell cycle arrest (a prelude to cell senescence), and p53-independent apoptosis occurred in the presence of 10-fold higher levels of ADR, consistent with previous studies. Taken together, these results reveal that the multiple roles of p53 in cell cycle regulation and apoptosis are first acquired during pluripotent stem cell differentiation.
l‐Carnitine protects against 1,4‐benzoquinone‐induced apoptosis and DNA damage by suppressing oxidative stress and promoting fatty acid oxidation in K562 cells
Authors: Sun Rongli , Man Zhaodi , Ji Jiahui , Ji Shuangbin , Xu Kai , Pu Yunqiu , Yu Linling , Zhang Juan , Yin Lihong , Pu Yuepu
Published: June 2020

Widespread occupational and environmental exposure to benzene is unavoidable and poses a public health threat. Studies of potential interventions to prevent or relieve benzene toxicity are, thus, essential. Research has shown l‐carnitine (LC) has beneficial effects against various pathological processes and diseases. LC possesses antioxidant activities and participates in fatty acid oxidation (FAO). In this study, we investigated whether 1,4‐benzoquinone (1,4‐BQ) affects LC levels and the FAO pathway, as well as analyzed the influence of LC on the cytotoxic effects of 1,4‐BQ. We found that 1,4‐BQ significantly decreased LC levels and downregulated Cpt1a, Cpt2, Crat, Hadha, Acaa2, and Acadvl mRNA expression in K562 cells. Subsequent assays confirmed that 1,4‐BQ decreased cell viability and increased apoptosis and caspase‐3, ‐8, and ‐9 activities. It also induced obvious oxidative stress and DNA damage, including an increase in the levels of reactive oxygen species and malondialdehyde, tail DNA%, and olive tail moment. Additionally, the mitochondrial membrane potential was significantly reduced. Cotreatment with LC (500 μmol/L) relieved these alterations by reducing oxidative stress and increasing the protein expression levels of Cpt1a and Hadha, particularly in the 20 μmol/L 1,4‐BQ group. Thus, our results demonstrate that 1,4‐BQ causes cytotoxicity, reduces LC levels, and downregulates the FAO genes. In contrast, LC exhibits protective effects against 1,4‐BQ‐induced apoptosis and DNA damage by decreasing oxidative stress and promoting the FAO pathway.
Understanding and assessing uncertainty of observational climate datasets for model evaluation using ensembles
Authors: Zumwald Marius , Knüsel Benedikt , Baumberger Christoph , Hirsch Hadorn Gertrude , Bresch David N. , Knutti Reto
Published: June 2020

In climate science, observational gridded climate datasets that are based on in situ measurements serve as evidence for scientific claims and they are used to both calibrate and evaluate models. However, datasets only represent selected aspects of the real world, so when they are used for a specific purpose they can be a source of uncertainty. Here, we present a framework for understanding this uncertainty of observational datasets which distinguishes three general sources of uncertainty: (1) uncertainty that arises during the generation of the dataset; (2) uncertainty due to biased samples; and (3) uncertainty that arises due to the choice of abstract properties, such as resolution and metric. Based on this framework, we identify four different types of dataset ensembles—parametric, structural, resampling, and property ensembles—as tools to understand and assess uncertainties arising from the use of datasets for a specific purpose. We advocate for a more systematic generation of dataset ensembles by using these sorts of tools. Finally, we discuss the use of dataset ensembles in climate model evaluation. We argue that a more systematic understanding and assessment of dataset uncertainty is needed to allow for a more reliable uncertainty assessment in the context of model evaluation. The more systematic use of such a framework would be beneficial for both scientific reasoning and scientific policy advice based on climate datasets.

This article is categorized under:

Paleoclimates and Current Trends > Modern Climate Change
Estrous cyclicity and reproductive success are unaffected by translocation for the formation of new reproductive pairs in captive red wolves (Canis rufus)
Authors: Franklin Ashley D. , Waddell William T. , Behrns Sue , Goodrowe Karen L.
Published: June 2020

This study investigated possible female‐related causes for inconsistent success among reproductive pairs in the zoo‐based red wolf (Canis rufus) population. Females (n = 13) at seven institutions were assessed for evidence of ovulation and normal reproductive cycles through the measurement of estradiol and progesterone metabolite excretion in feces. Fecal cortisol metabolites (FCM) were also measured. Factors potentially affecting FCM and/or estrous cyclicity were recorded, including exhibit status (on vs. off), reproductive history (proven vs. unproven), copulatory behaviors (ties observed: yes or no), pregnancy/parturition (pups or no pups produced), and translocation before the observed breeding season (yes or no). No differences were observed in baseline FCM between females housed on versus off‐exhibit (p = .46) or between females producing pups and those who did not (p = .19). Baseline FCM were significantly lower among females observed in copulatory ties compared to females never observed in a tie (p = .04), and tended to be higher in females translocated before the breeding season compared to females in existing reproductive pairs (p = .11), and among historically unproven females compared to proven females (p = .11). All females evaluated had an endocrine profile indicative of ovulation and among the four females translocated to be paired with a new male before the breeding season, two had successful pregnancies, producing litters. Therefore, despite observed differences in baseline FCM among factors, estrous cyclicity and reproductive success are unaffected by translocation for the formation of new reproductive pairs in the zoo‐based red wolf population.
Predator personality and prey detection: inter-individual variation in responses to cryptic and conspicuous prey
Authors: Szopa-Comley Andrew W. , Donald William Gregory , Ioannou Christos C.
Published: June 2020

Limited attention constrains predators from engaging in cognitively demanding tasks such as searching for cryptic prey at the same time as remaining vigilant towards threats. Since finite attention can result in negative correlations between foraging and vigilance, the tendency of individual predators to focus attention on searching for cryptic prey may be correlated with other behavioural traits which reflect risk-reward trade-offs, such as consistent inter-individual variation in boldness (a personality trait describing risk-taking, defined in this study as the time taken to leave a refuge). We investigated the importance of personality in prey detection by comparing inter-individual variation in the response of three-spined sticklebacks (Gasterosteus aculeatus) to conspicuous and cryptic prey. Fish were slower to attack cryptic prey than conspicuous prey, consistent with cryptic prey being harder to detect. Despite the greater challenge involved in detecting cryptic prey, inter-individual variation in the time taken to detect prey was similar in the cryptic and conspicuous prey treatments, and was uncorrelated with boldness, which was repeatable between individuals. We also observed a positive association between the rate of attack on conspicuous prey and whether individual fish attacked cryptic prey in other trials. Our findings suggest that boldness is not related to prey detection or attention in this context. Instead, consistent differences in motivation once exploration has begun between individual predators may explain inter-individual variation in the time taken to attack both prey cryptic and conspicuous prey.

Significance statement

Using an experimental approach to manipulate the conspicuousness of prey, we show that individual fish consistently differ in their rates of attacking prey. This demonstrates that fish show “personality variation” in predatory behaviour, but these inter-individual differences were not related to the boldness of each fish (their tendency to engage in risky behaviours).
A Numerical Study on Thermal Elastohydrodynamic Lubrication of Coated Polymers
Authors: Ziegltrum A. , Maier E. , Lohner T. , Stahl K.
Published: June 2020

The application of polymers in power-transmitting machine elements, e.g., gears, is limited by moderate thermo-mechanical properties and the detrimental accumulation of contact heat, even with external lubrication. Hence, polymer rolling–sliding elements are often prone to thermo-mechanical overload or abrasive wear. Diamond-like carbon (DLC) coatings are well known from steel applications for enhancing wear resistance and reducing friction. Since preliminary results indicate promising results for such coatings for polymers as well, their influence on the behavior of lubricated polymer contacts is investigated by numerical simulation. For polymer–steel contacts, the mechanical and thermophysical properties of coating and polymer are varied. The contact geometry is dominated by a local conformity, in which most of the deformation is related to the polymer. The DLC coatings affect film thickness and hydrodynamic pressure only little even for untypical high coating thicknesses. In contrast, the contact temperature decreases already for very thin coatings due to enhanced heat removal. Hence, DLC coatings can act as a thermal barrier protecting the polymer from detrimental heat and protecting the polymer from abrasive wear.
Biomass Production of Prairie Cordgrass (Spartina pectinata Link.) Using Urea and Kura Clover (Trifolium ambiguum Bieb.) as a Source of Nitrogen
Authors: Kim SungUn , Albrecht Kenneth , Sheaffer Craig , Lee Dokyoung , Subramanian Senthil , Owens Vance
Published: June 2020

Optimizing nitrogen (N) management is an important factor for sustainable perennial biomass systems. However, N application is costly, both financially and environmentally. Our objectives were to determine: (1) N rate and plant spacing effects on yield and yield components of prairie cordgrass swards and (2) fertilizer N replacement value (FNRV) of kura clover in prairie cordgrass-kura clover binary mixtures. Plots were established in Illinois, Minnesota, South Dakota, and Wisconsin, USA, in 2010. Kura clover was transplanted on 30-cm centers in all treatments in which it was a component; prairie cordgrass seedlings were transplanted within the kura clover on 60- and 90-cm centers. Monoculture prairie cordgrass stands were established at the same population densities of mixed stands and fertilized with 0, 75, 150, or 225 kg N ha-1. Biomass was harvested in the autumn from 2011 to 2013. N (urea), year, plant spacing, and year × plant spacing affected prairie cordgrass production at all locations. Prairie cordgrass yield increased with N application, but the response varied by location. N application tended to increase prairie cordgrass tiller density and consistently increased tiller mass. Prairie cordgrass yield with 0 N was equal to or less than the yield of prairie cordgrass/kura clover mixtures at all locations in 2011 and 2012; however, kura clover provided a FNRV of 25–82 kg N ha-1 to prairie cordgrass in 2013. Kura clover has potential to provide N to prairie cordgrass in binary mixtures of these two species and on land that may not be easily farmed due to wetness.
An Indian national survey of therapeutic drug monitoring with anti-tumor necrosis (TNF) medications in inflammatory bowel disease
Authors: Patel Rajan N. , Nigam Gaurav B. , Jatale Raj G. , Desai Devendra , Makharia Govind , Ahuja Vineet , Limdi Jimmy K.
Published: June 2020

Background

Evidence supports therapeutic drug monitoring (TDM) in improving efficacy and cost-effectiveness of anti-TNF therapy in inflammatory bowel disease (IBD). Data on perceptions and barriers to TDM use are limited and no data are available from India. Our objective was to assess clinicians’ attitudes and barriers to TDM use in IBD.

Methods

A 16-question survey was distributed to members of the Indian Society of Gastroenterology. Information on clinician characteristics, demographics, use and barriers towards TDM with anti-TNFs was collected. Logistic regression was used to predict factors influencing TDM use.

Results

Two hundred and forty-two respondents participated (92.5% male); 83% were consultant gastroenterologists. Of 104 respondents meeting inclusion criteria (treating > 5 IBD patients and at least 1 with an anti-TNF per month), complete responses were available for 101 participants. TDM was utilized by 20% (n = 20) of respondents. Of them, 89.5% (n = 17) used TDM for secondary loss of response; 73.7% (n = 14) for primary non-response and 5.3% (n = 1) proactively. Barriers to TDM use were cost (71.2%), availability (67.8%), time lag in results (58.7%) and the perception that TDM is time-consuming (45.7%). Clinicians treating > 30 IBD patients were more likely to check TDM (OR = 4.9, p = 0.02). Of 81 respondents not using TDM, 97.5% (n = 79) would do so if all the barriers were removed.

Conclusion

Significant barriers to TDM use were availability, cost and time lag for results. If these barriers were removed, almost all the clinicians would use TDM at least reactively and 25% would use proactively. There is an urgent need to address these barriers and optimize anti-TNF therapy for optimal outcomes.
Technologies of Trauma: Flesh Witnessing to Livestreaming Online
Authors: Ibrahim Yasmin
Published: June 2020

This paper examines the trajectory of witnessing and testimony through modernity, utilizing the notion of ‘technologies of trauma’. Such a notion acknowledges humanity’s reliance on technologies to transmute history, trauma, or memory, transforming technologies from medium and machine into artefacts for circulation and exchange. The transcendence of medium into artefacts as sites of trauma equally highlights the socio-political frames within which testimony is extracted and witnessing is enacted, unleashing trauma as a cultural form and a resonant genre of popular consumption. This paper, in tracing the trajectory of these technologies of trauma and its cultural turn from the sensorial to mass witnessing to the aesthetic regimes of immediacy in the digital age, comes to term with our machinic bind in releasing trauma and its aestheticization as a cultural artefact and the unethical challenges, which unfold in such a proposition. The development of print, photography, television, and digital platforms as technologies of trauma reiterates the popularization of trauma, witnessing and testimony as cultural forms reiterating how these are intimately implicated in our emergence as active consuming communities of trauma and in tandem how these remake us as vulnerable subjects through the circulation of trauma as a cultural form.
Conserved quantities and Hamiltonization of nonholonomic systems
Authors: Balseiro Paula , Yapu Luis P.
Published: June 2020

This paper studies hamiltonization of nonholonomic systems using geometric tools, building on [1,5]. The main novelty in this paper is the use of symmetries and suitable first integrals of the system to explicitly define a new bracket on the reduced space that codifies the nonholonomic dynamics and carries, additionally, an almost symplectic foliation (determined by the common level sets of the first integrals); in particular cases of interest, this new bracket is a Poisson structure that hamiltonizes the system. Our construction of the new bracket is based on a gauge transformation of the nonholonomic bracket by a global 2-form that we explicitly describe. We study various geometric features of the reduced brackets and apply our formulas to obtain a geometric proof of the hamiltonization of a homogeneous ball rolling without sliding in the interior side of a convex surface of revolution.
Environmental Human Rights in Earth System Governance
Authors: Baber Walter F. , Bartlett Robert V.
Published: June 2020

Environmental rights are a category of human rights necessarily central to both democracy and effective earth system governance (any environmental-ecological-sustainable democracy). For any democracy to remain democratic, some aspects must be beyond democracy and must not be allowed to be subjected to any ordinary democratic collective choice processes shy of consensus. Real, established rights constitute a necessary boundary of legitimate everyday democratic practice. We analyze how human rights are made democratically and, in particular, how they can be made with respect to matters environmental, especially matters that have import beyond the confines of the modern nation state.
Torn between Legal Claiming and Privatized Remedy: Rights Mobilization against Gold Mining in Chile
Authors: Maher Rajiv , Monciardini David , Böhm Steffen
Published: June 2020

Many academic authors, policy makers, NGOs, and corporations have focused on top-down human rights global norm-making, such as the United Nations Guiding Principles for Business and Human Rights (UNGPs). What is often missing are contextual and substantive analyses that interrogate rights mobilization and linkages between voluntary transnational rules and domestic governance. Deploying a socio-legal approach and using a combination of longitudinal field and archival data, this article investigates how a local, indigenous community in Northern Chile mobilized their rights over a period of almost two decades. We found that rights mobilization was largely shaped by tensions between the different logics of legality and the business organization. In our case, the UNGP implementation process has been ineffective in giving rightsholders access to genuine remedy. On the contrary, it has led to weakened rights mobilization, dividing the local community. We conclude that greater attention to rights mobilization and domestic governance dynamics should be given in the business and human rights debate.
Safety of ACE-I and ARB medications in COVID-19: A retrospective cohort study of inpatients and outpatients in California
Authors: Rubin Samuel J. S. , Falkson Samuel R. , Degner Nicholas R. , Blish Catherine A.
Published: June 2020

Introduction:

There is significant interest in the use of angiotensin converting enzyme inhibitors (ACE-I) and angiotensin II receptor blockers (ARB) in coronavirus disease 2019 (COVID-19) and concern over potential adverse effects since these medications upregulate the severe acute respiratory syndrome coronavirus 2 host cell entry receptor ACE2. Recent studies on ACE-I and ARB in COVID-19 were limited by excluding outpatients, excluding patients by age, analyzing ACE-I and ARB together, imputing missing data, and/or diagnosing COVID-19 by chest computed tomography without definitive reverse transcription polymerase chain reaction (RT-PCR), all of which are addressed here.

Methods:

We performed a retrospective cohort study of 1023 COVID-19 patients diagnosed by RT-PCR at Stanford Hospital through April 8, 2020 with a minimum follow-up time of 14 days to investigate the association between ACE-I or ARB use with outcomes.

Results:

Use of ACE-I or ARB medications was not associated with increased risk of hospitalization, intensive care unit admission, or death. Compared to patients with charted past medical history, there was a lower risk of hospitalization for patients on ACE-I (odds ratio (OR) 0.43; 95% confidence interval (CI) 0.19–0.97; P = 0.0426) and ARB (OR 0.39; 95% CI 0.17–0.90; P = 0.0270). Compared to patients with hypertension not on ACE-I or ARB, patients on ARB medications had a lower risk of hospitalization (OR 0.09; 95% CI 0.01–0.88; P = 0.0381).

Conclusions:

These findings suggest that the use of ACE-I and ARB is not associated with adverse outcomes and may be associated with improved outcomes in COVID-19, which is immediately relevant to care of the many patients on these medications.
Employers as Subjects of the Immigration State: How the State Foments Employment Insecurity for Temporary Immigrant Workers
Authors: Gleeson Shannon , Griffith Kati L.
Published: June 2020

The state plays a key role in shaping worker precarity, and employers are key actors in mediating this process. While employers sometimes may act as willing extensions of the deportation machinery, they are also subjects of the immigration state. In this article, we highlight the impact of state-employer dynamics on migrant workers with Temporary Protected Status (TPS). These workers have only provisional permission to live and work in the United States, but are not tied to any single employer. Even though they are privileged over unauthorized workers and employer-sponsored guest workers, TPS holders experience their own brand of state-induced precarity. Their employers risk civil or criminal liability if they are not in compliance with work authorization requirements and must repeatedly navigate an unpredictable and confusing immigration bureaucracy. Drawing on interviews with 121 low-wage TPS workers and two dozen of their advocates in the New York City metropolitan area, our findings reveal that the intertwined coercive and bureaucratic arms of the immigration state together make hiring TPS workers a more risky and costly proposition for employers, thereby exacerbating the job insecurity that TPS workers already face due to an at-will employment regime that offers few protections against firing.
Measuring directed triadic closure with closure coefficients
Authors: Yin Hao , Benson Austin R. , Ugander Johan
Published: June 2020

Recent work studying triadic closure in undirected graphs has drawn attention to the distinction between measures that focus on the “center” node of a wedge (i.e., length-2 path) versus measures that focus on the “initiator,” a distinction with considerable consequences. Existing measures in directed graphs, meanwhile, have all been center-focused. In this work, we propose a family of eightdirected closure coefficientsthat measure the frequency of triadic closure in directed graphs from the perspective of the node initiating closure. The eight coefficients correspond to different labeled wedges, where the initiator and center nodes are labeled, and we observe dramatic empirical variation in these coefficients on real-world networks, even in cases when the induced directed triangles are isomorphic. To understand this phenomenon, we examine the theoretical behavior of our closure coefficients under a directed configuration model. Our analysis illustrates an underlying connection between the closure coefficients and moments of the joint in- and out-degree distributions of the network, offering an explanation of the observed asymmetries. We also use our directed closure coefficients as predictors in two machine learning tasks. We find interpretable models with AUC scores above 0.92 in class-balanced binary prediction, substantially outperforming models that use traditional center-focused measures.
PROPERTIES OF FINITE GROUPS DETERMINED BY THE PRODUCT OF THEIR ELEMENT ORDERS
Authors: BANIASAD AZAD MORTEZA , KHOSRAVI BEHROOZ
Published: June 2020

For a finite group$G$, define$l(G)=(\prod _{g\in G}o(g))^{1/|G|}/|G|$, where$o(g)$denotes the order of$g\in G$. We prove that if$l(G)>l(A_{5}),l(G)>l(A_{4}),l(G)>l(S_{3}),l(G)>l(Q_{8})$or$l(G)>l(C_{2}\times C_{2})$, then$G$is solvable, supersolvable, nilpotent, abelian or cyclic, respectively.
Dietary inflammatory potential in relation to the gut microbiome: results from a cross-sectional study
Authors: Zheng Jiali , Hoffman Kristi L. , Chen Jiun-Sheng , Shivappa Nitin , Sood Akhil , Browman Gladys J. , Dirba Danika D. , Hanash Samir , Wei Peng , Hebert James R. , Petrosino Joseph F. , Schembre Susan M. , Daniel Carrie R.
Published: June 2020

Diet has direct and indirect effects on health through inflammation and the gut microbiome. We investigated total dietary inflammatory potential via the literature-derived index (Dietary Inflammatory Index (DII®)) with gut microbiota diversity, composition and function. In cancer-free patient volunteers initially approached at colonoscopy and healthy volunteers recruited from the medical centre community, we assessed 16S ribosomal DNA in all subjects who provided dietary assessments and stool samples (n 101) and the gut metagenome in a subset of patients with residual fasting blood samples (n 34). Associations of energy-adjusted DII scores with microbial diversity and composition were examined using linear regression, permutational multivariate ANOVA and linear discriminant analysis. Spearman correlation was used to evaluate associations of species and pathways with DII and circulating inflammatory markers. Across DII levels, α- and β-diversity did not significantly differ; however, Ruminococcus torques, Eubacterium nodatum, Acidaminococcus intestini and Clostridium leptum were more abundant in the most pro-inflammatory diet group, while Akkermansia muciniphila was enriched in the most anti-inflammatory diet group. With adjustment for age and BMI, R. torques, E. nodatum and A. intestini remained significantly associated with a more pro-inflammatory diet. In the metagenomic and fasting blood subset, A. intestini was correlated with circulating plasminogen activator inhibitor-1, a pro-inflammatory marker (rho = 0·40), but no associations remained significant upon correction for multiple testing. An index reflecting overall inflammatory potential of the diet was associated with specific microbes, but not overall diversity of the gut microbiome in our study. Findings from this preliminary study warrant further research in larger samples and prospective cohorts.
Dietary methionine supplementation improves the European seabass (Dicentrarchus labrax) immune status following long-term feeding on fishmeal-free diets
Authors: Machado Marina , Engrola Sofia , Colen Rita , Conceição Luis E. C. , Dias Jorge , Costas Benjamín
Published: June 2020

Methionine is a limiting amino acid (AA) in fish diets, particularly in those containing high levels of plant protein (PP), and is key in the immune system. Accordingly, outcome on the fish immune mechanisms of methionine-deficient and methionine-supplemented diets within the context of 0 % fishmeal formulation, after a short and prolonged feeding period, was studied in European seabass (Dicentrarchus labrax). For this, seabass juveniles were fed a (i) fishmeal-free diet, meeting AA requirements, but deficient in methionine (MET0·65); (ii) as control, the MET0·65 supplemented withl-methionine at 0·22 % of feed weight (CTRL); (iii) two diets, identical to MET0·65 but supplemented at 0·63 and 0·88 % of feed weight ofl-methionine (MET1·25 and MET1·5, respectively); and (iv) a fishmeal-based diet (FM), as positive control. After 2 and 12 weeks of feeding, blood and plasma were sampled for leucocyte counting and humoral parameter assays and head-kidney collected for gene expression. After 2 weeks of feeding, a fishmeal-free diet supplemented with methionine led to changes in the expression of methionine- and leucocyte-related genes. A methionine immune-enhancer role was more evident after 12 weeks with an increased neutrophil percentage and a decreased expression of apoptotic genes, possibly indicating an enhancement of fish immunity by methionine dietary supplementation. Furthermore, even though CTRL and FM present similar methionine content, CTRL presented a reduced expression of several immune-related genes indicating that in a practical PP-based diet scenario, the requirement level of methionine for an optimal immune status could be higher.
Molecular characterization ofEchinococcus granulosusin livestock of Al-Madinah (Saudi Arabia)
Authors: AL-Mutairi N.M. , Taha H.A. , Nigm A.H.
Published: June 2020

Echinococcus granulosusis the causative agent of cystic echinococcosis, which has serious impacts on human and/or animal health, resulting in significant economic losses.Echinococcus granulosuscomprises a number of intra-specific variants or strains at the genetic level. In Saudi Arabia, few studies were performed on genetic variations inEchinococcusspecies. Therefore, the present study aimed to investigate the phenotypic and genetic characterization of hydatid cysts harboured by sheep and camels in Al-Madinah Al-Munawarah. Samples of hydatid cysts were collected from local sheep (n= 25) and camels (n= 8). The morphological criteria of protoscoleces were investigated. To investigate the molecular characterization, random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR), single-stranded conformation polymorphism (SSCP) were carried out. DNA was extracted from individual fertile cysts and subjected to RAPD-PCR analysis (using five arbitrary primers) and PCR amplification of cytochromecoxidase I (cox1) and 12S ribosomal ribonucleic acid (12S rRNA) genes. The PCR products were subjected to SSCP analysis for genetic discrimination inE. granulosusisolates. In addition, partially sequencing of the mitochondrial DNAcox1genes was achieved for assessing the phylogenetic positions of collected isolates using some global published sequence data ofcox1genes. The rostellar hooks of camel and local sheep isolates show remarkable variability in their dimensions. Five distinct SSCP patterns were identified in the 12S rRNA gene, showing intraspecific variations inE. granulosusof camels and local sheep. Sequencing of (cox1) genes of both local sheep and camels exhibit high similarity with those of the same gene (E. granulosus sensu stricto) published in NCBI BLAST.
Open Policy Making in the UK – to Whom Might Policy Formulation be ‘Opening Up’?
Authors: EXLEY SONIA
Published: June 2020

This article explores recent UK government aspirations towards ‘open policy making’ (OPM). Against a backdrop of scholarly literatures on power inequalities in policy making, I consider to whom processes of policy formulation under a banner of OPM are expected to be ‘opening up’. The article draws on an analysis of government documents from 2012–2018 plus some supplementary data from expert interviews. It notes aspirations towards ‘opening up’ policy formulation to new experts and a particular preoccupation with encouraging private sector involvement. Ideas which may boost ordinary citizens’ input are also part of what ‘makes up’ UK Government OPM, though citizen involvement appears restricted, sitting uneasily alongside commitments to austerity influencing how ‘openness’ is understood.
SECOND LANGUAGE USERS EXHIBIT SHALLOW MORPHOLOGICAL PROCESSING
Authors: Song Yoonsang , Do Youngah , Thompson Arthur L. , Waegemaekers Eileen R. , Lee Jongbong
Published: June 2020

The present study tests the Shallow Structure Hypothesis (SSH), which claims that compared to L1 processing, L2 language processing generally underuses grammatical information, prioritizing nongrammatical information. Specifically, this cross-modal priming study tests SSH at the level of morphology, investigating whether late advanced L2 learners construct hierarchically structured representations for trimorphemic derived words during real-time processing as native speakers do. Our results support SSH. In lexical decision on English trimorphemic words (e.g., unkindness or [[un-[kind]]-ness]), L1 recognition of the targets was facilitated by their bimorphemic morphological-structural constituent primes (e.g., unkind), but not by their bimorphemic nonconstituent primes (e.g., kindness), which were only semantically and formally related to the target. In contrast, L2 recognition was equally facilitated by both constituent and nonconstituent primes. These results suggest that unlike L1 processing, L2 processing of multimorphemic words is not mainly guided by detailed morphological structure, overrelying on nonstructural information.
Using theEighteenth-Century English Phonology Database(ECEP) as a teaching resource
Authors: WALLIS CHRISTINE
Published: June 2020

This article reports on the use of theEighteenth-Century English Phonology Database(ECEP) as a teaching resource in historical sociolinguistics and historical linguistics courses at the University of Sheffield. Pronouncing dictionaries are an invaluable resource for students learning about processes of standardisation and language attitudes during the Late Modern English period (1700–1900), however they are not easy to use in their original format. Each author uses their own notation system to indicate their recommended pronunciation, while the terminology used to describe the quality of the vowels and consonants differs from that used today, and provides an additional obstacle to the student wishing to interrogate such sources. ECEP thus provides a valuable intermediary between the students and the source material, as it includes IPA equivalents for the recommended pronunciations, as well as any metalinguistic commentary offered by the authors about a particular pronunciation. This article demonstrates a teaching approach that not only uses ECEP as a tool in its own right, but also explores how it can be usefully combined with other materials covering language change in the Late Modern English period to enable students to undertake their own investigations in research-led courses.
Further realization of a flexible metamaterial-based antenna on indium nickel oxide polymerized palm fiber substrates for RF energy harvesting
Authors: Elwi Taha A. , Al-Saegh Ali M.
Published: June 2020

This paper discusses the design and fabrication of a low-profile Hilbert-shaped metamaterial (MTM) array-based antenna, forming a rectangular patch with partial ground plane backing; the rest is slotted with traces for RF energy harvesting. The antenna is mounted on a 28 mm × 32 mm indium nickel oxide polymerized palm fiber (INP) substrate and compared to the identical one based on FR4 substrate. The two prototypes are printed with silver nanoparticles. Numerical and experimental tests are applied to the antenna performance in terms of S11 and radiation patterns. The obtained antenna gain bandwidth product of the INP prototype is found to be significantly better than the FR4 prototype. The proposed INP antenna gain at 5.8 and 8 GHz frequencies is found to be about 4.56 and 7.38 dBi, respectively, while the FR4 antenna gain is found to be 4.56 and 6.85 dBi at 5.8 and 8 GHz, respectively. Finally, the resultant DC voltage and the efficiency of conversion from harvested RF energy are measured experimentally at 5.8 and 8 GHz for both proposed prototypes.
High gain broadside mode operation of a cylindrical dielectric resonator antenna using simple slot excitation
Authors: Kumar Ojha Anuj , Praveen Kumar A. V.
Published: June 2020

In this work, the authors report the operation of a cylindrical dielectric resonator antenna (CDRA) in the high gain HEM13δ mode, for the first time. This mode, excited with a standard microstrip slot, radiates in the broadside direction with gain in the range of 8−10 dBi. It is shown that through feed optimization, the HEM13δ mode can be excited dominantly by suppressing the fundamental HEM11δ mode of the CDRA. Detailed simulation studies show that the HEM13δ mode is supported by cylindrical dielectric resonators with an aspect ratio (radius to height ratio or a/d) >1, and it resonates at a frequency approximately 2.2 times that of the fundamental HEM11δ mode. The above features of the HEM13δ mode CDRA can be used as approximate design rules. For a CDRA with dielectric constant ɛr = 24, diameter 2a = 19.43 mm, and height d = 7.3 mm (a/d = 1.3), the HEM13δ mode is excited at 6.125 GHz with a peak gain of 10.14 dBi in simulation. Corresponding values from prototype measurement are 5.981 GHz and 9.62 dBi, respectively for the resonant frequency and the gain, verifying the simulation.
Low Friction Coefficient of Phyllosilicate Fault Gouges and the Effect of Humidity: Insights From a New Microphysical Model
Authors: den Hartog S. A. M. , Faulkner D. R. , Spiers C. J.
Published: June 2020

Fault slip is often localized in phyllosilicate‐rich fault gouges in a manner consistent with the relatively low friction coefficients measured for dry and especially wet phyllosilicates in laboratory experiments. However, the microphysics controlling these low friction coefficients remains unclear. Here, we propose a microphysical model, inspired by microstructural observations, for the prediction of the absolute value of the friction coefficient of pure dry and wet phyllosilicates. Experimentally produced phyllosilicate gouges suggest that shearing is controlled by sliding along (001) grain/platelet interfaces operating in series with removal of overlapping grain edge barriers by basal cleavage. We derive a model incorporating a subcritical crack propagation equation for the latter, constrained by subcritical crack growth data for muscovite. Model predictions for muscovite show similar trends regarding the effects of humidity and slip velocity on friction coefficient as do experiments at room temperature. The absolute value predicted for the friction coefficient is difficult to compare with experimental values, as it critically depends on atomic scale (001) sliding resistance, which is poorly constrained by available experimental data. Further discrepancies with experimental data can be explained by effects of varying grain size, grain aspect ratio, and porosity on the friction coefficient. While numerous qualitative explanations have been proposed previously for the low friction coefficient exhibited by phyllosilicates, especially in the presence of water, our study provides a new step toward a quantitative, physically based model.
The Case for an Open Water Balance: Re‐envisioning Network Design and Data Analysis for a Complex, Uncertain World
Authors: Kampf Stephanie K. , Burges Stephen J. , Hammond John C. , Bhaskar Aditi , Covino Tim P. , Eurich Abby , Harrison Hannah , Lefsky Michael , Martin Caroline , McGrath Daniel , Puntenney‐Desmond Kira , Willi Kathryn
Published: June 2020

The discipline of hydrology has long focused on quantifying the water balance, which is frequently used to estimate unknown water fluxes or stores. While technologies for measuring water balance components continue to improve, all components of the balance have substantial uncertainty at the watershed scale. Watershed‐scale evapotranspiration, storage, and groundwater import or export are particularly difficult to measure. Given these uncertainties, analyses based on assumed water balance closure are highly sensitive to uncertainty propagation and errors of omission, where unknown components are assumed negligible. This commentary examines how greater insight may be gained in some cases by keeping the water balance open rather than applying methods that impose water balance closure. An open water balance can facilitate identifying where unknowns such as groundwater import/export are affecting watershed‐scale streamflow. Strategic improvements in monitoring networks can help reduce uncertainties in observable variables and improve our ability to quantify unknown parts of the water balance. Improvements may include greater spatial overlap between measurements of water balance components through coordination between entities responsible for monitoring precipitation, snow, evapotranspiration, groundwater, and streamflow. Measuring quasi‐replicate watersheds can help characterize the range of variability in the water balance, and nested measurements within watersheds can reveal areas of net groundwater import or export. Well‐planned monitoring networks can facilitate progress on critical hydrologic questions about how much water becomes evapotranspiration, how groundwater interacts with surface watersheds at varying spatial and temporal scales, how much humans have altered the water cycle, and how streamflow will respond to future climate change.
Reply to Comment by Heilbron and Valeriano on “Tectono Metamorphic Evolution of the Central Ribeira Belt, Brazil: A Case of Late Neoproterozoic Intracontinental Orogeny and Flow of Partially Molten Deep Crust During the Assembly of West Gondwana”
Authors: Meira Vinícius T. , Garcia‐Casco Antonio , Hyppolito Thaís , Juliani Caetano , Schorscher Johann Hans D.
Published: June 2020

The exercise of paleogeographic/tectonic reconstruction of past orogenic belts is a complex task that includes the interpretation and integration of multi‐technique approaches such as basin tectonics, structural geology, petrology, geochemistry, geochronology, and geodynamics. Proterozoic geologic records are fragmented and incomplete, which make efforts to reconstruct paleogeography and orogenic processes even more challenging. Therefore, for our understanding of the tectonic evolution of past orogenic systems to advance, it is essential that any existing model, including the ones proposed by Heilbron and Valeriano (2020, https://doi.org/10.1029/2019tc005897), are repeatedly exposed to debate and testing. In this reply we address the points raised by Heilbron and Valeriano in order to clarify the scientific foundations of our tectonic interpretation for one of the Neoproterozoic Brasiliano/Pan‐African orogens in South America (the Central Ribeira Belt), presented in Meira, Garcia‐Casco, Hyppolito, et al. (2019, https://doi.org/10.1029/2018TC004959). We also evaluate the proposed paleogeographic reconstructions for this part of West Gondwana and conclude that an intracontinental model better explains the currently available data and observations, including the space problem pointed out in recent publications, and that existing geochemical and geochronologic data by themselves are not conclusive with respect to an unequivocal tectonic environment.
Novel diamond shuttle to deliver flexible neural probe with reduced tissue compression
Authors: Na Kyounghwan , Sperry Zachariah J. , Lu Jiaao , Vöröslakos Mihaly , Parizi Saman S. , Bruns Tim M. , Yoon Euisik , Seymour John P.
Published: June 2020

The ability to deliver flexible biosensors through the toughest membranes of the central and peripheral nervous system is an important challenge in neuroscience and neural engineering. Bioelectronic devices implanted through dura mater and thick epineurium would ideally create minimal compression and acute damage as they reach the neurons of interest. We demonstrate that a three-dimensional diamond shuttle can be easily made with a vertical support to deliver ultra-compliant polymer microelectrodes (4.5-µm thick) through dura mater and thick epineurium. The diamond shuttle has 54% less cross-sectional area than an equivalently stiff silicon shuttle, which we simulated will result in a 37% reduction in blood vessel damage. We also discovered that higher frequency oscillation of the shuttle (200 Hz) significantly reduced tissue compression regardless of the insertion speed, while slow speeds also independently reduced tissue compression. Insertion and recording performance are demonstrated in rat and feline models, but the large design space of these tools are suitable for research in a variety of animal models and nervous system targets.
Cheaters shape the evolution of phenotypic heterogeneity in Bacillus subtilis biofilms
Authors: Martin Marivic , Dragoš Anna , Otto Simon B , Schäfer Daniel , Brix Susanne , Maróti Gergely , Kovács Ákos T
Published: June 2020

Biofilms are closely packed cells held and shielded by extracellular matrix composed of structural proteins and exopolysaccharides (EPS). As matrix components are costly to produce and shared within the population, EPS-deficient cells can act as cheaters by gaining benefits from the cooperative nature of EPS producers. Remarkably, genetically programmed EPS producers can also exhibit phenotypic heterogeneity at single-cell level. Previous studies have shown that spatial structure of biofilms limits the spread of cheaters, but the long-term influence of cheating on biofilm evolution is not well understood. Here, we examine the influence of EPS nonproducers on evolution of matrix production within the populations of EPS producers in a model biofilm-forming bacterium, Bacillus subtilis. We discovered that general adaptation to biofilm lifestyle leads to an increase in phenotypical heterogeneity of eps expression. However, prolonged exposure to EPS-deficient cheaters may result in different adaptive strategy, where eps expression increases uniformly within the population. We propose a molecular mechanism behind such adaptive strategy and demonstrate how it can benefit the EPS producers in the presence of cheaters. This study provides additional insights on how biofilms adapt and respond to stress caused by exploitation in long-term scenario.
Parallel reductive genome evolution in Desulfovibrio ectosymbionts independently acquired by Trichonympha protists in the termite gut
Authors: Takeuchi Mariko , Kuwahara Hirokazu , Murakami Takumi , Takahashi Kazuki , Kajitani Rei , Toyoda Atsushi , Itoh Takehiko , Ohkuma Moriya , Hongoh Yuichi
Published: June 2020

Several Trichonympha protist species in the termite gut have independently acquired Desulfovibrio ectosymbionts in apparently different stages of symbiosis. Here, we obtained the near-complete genome sequence of Desulfovibrio phylotype ZnDsv-02, which attaches to the surface of Trichonympha collaris cells, and compared it with a previously obtained genome sequence of ‘Candidatus Desulfovibrio trichonymphae’ phylotype Rs-N31, which is almost completely embedded in the cytoplasm of Trichonympha agilis. Single-nucleotide polymorphism analysis indicated that although Rs-N31 is almost clonal, the ZnDsv-02 population on a single host cell is heterogeneous. Despite these differences, the genome of ZnDsv-02 has been reduced to 1.6 Mb, which is comparable to that of Rs-N31 (1.4 Mb), but unlike other known ectosymbionts of protists with a genome similar in size to their free-living relatives. Except for the presence of a lactate utilization pathway, cell-adhesion components and anti-phage defense systems in ZnDsv-02, the overall gene-loss pattern between the two genomes is very similar, including the loss of genes responsive to environmental changes. Our study suggests that genome reduction can occur in ectosymbionts, even when they can be transmitted horizontally and obtain genes via lateral transfer, and that the symbiont genome size depends heavily on their role in the symbiotic system.