bluuebunny/crossref_metadata_2025 huggingface dataset:
Higher Serum Brain-Derived Neurotrophic Factor Levels Are Associated With a Lower Risk of Cognitive Decline: A 2-Year Follow Up Study in Community-Dwelling Older Adults
Authors: Fujiwara Yoshinori , Ihara Kazushige , Hachisu Mitsugu , Suzuki Hiroyuki , Kawai Hisashi , Sakurai Ryota , Hirano Hirohiko , Chaves Paulo H. M. , Hashizume Masahiro , Obuchi Shuichi
Published: June 2021

Objective

To assess the relationship of serum brain-derived neurotrophic factor (BDNF) levels with the subsequent short-term decline in cognitive functioning in community-dwelling older adults.

Design

Two-year prospective, observational study.

Setting and Participants

The study included 405 adults aged 65–84 years, initially free of a dementia diagnosis who were living in Tokyo, Japan.

Methods

Participants underwent health assessments at baseline (2011) and follow-up (2013). Serum BDNF levels and scores from the Montreal Cognitive Assessment-Japanese version (MoCA-J) were systematically measured. Logistic regression was used to estimate the odds of cognitive decline between baseline and follow-up assessments in the full MoCA-J scale (operationally defined as a decrease of two or more points), as well as in MoCA-J subscales (decline of one or more points in a specific subscale), as a function of serum BDNF level, adjusting for baseline demographics, prevalent chronic diseases, and baseline cognitive scores.

Results

Among individuals who performed worse on the full MoCA-J at baseline (i.e., scores in the bottom quartile [≤21], which is consistent with a mild cognitive impairment status), but not among those who performed better (top 3 quartiles), those with highest baseline serum BDNF levels (top quartile) had lower odds of subsequent decline in the full MoCA-J scale than those with lowest (bottom quartile); i.e., odds ratio (OR): 0.10 (95% confidence interval [CI]: 0.02–0.62; p = 0.013). Regarding MoCA-J subscales, adjusted odds of decline in the executive function subscale, but not in the other five subscales, were substantially low among those with highest baseline serum BDNF levels (top quartile), as compared to those with the lowest (bottom quartile), i.e., OR: 0.27 (95% CI:0.13–0.60; p < 0.001).

Conclusion and Implications

Higher serum BDNF levels were associated with a lower risk of decline in cognitive function in a sample of community-dwelling older Japanese adults. Risk varied across cognitive subdomains and according to baseline cognition. This warrants further research to evaluate the added-value of serum BDNF in health promotion initiatives directed toward cognitive decline prevention in community-dwelling older adults.
Role of Brain Arterial Remodeling in HIV-Associated Cerebrovascular Outcomes
Authors: Spagnolo-Allende Antonio , Gutierrez Jose
Published: June 2021

As the life expectancy of people living with HIV (PLWH) on combination antiretroviral therapy (cART) increases, so does morbidity from cerebrovascular disease and neurocognitive disorders. Brain arterial remodeling stands out as a novel investigational target to understand the role of HIV in cerebrovascular and neurocognitive outcomes. We therefore conducted a review of publications in PubMed, EMBASE, Web of Science and Wiley Online Library, from inception to April 2021. We included search terms such as HIV, cART, brain, neuroimmunity, arterial remodeling, cerebrovascular disease, and neurocognitive disorders. The literature shows that, in the post-cART era, PLWH continue to experience an increased risk of stroke and neurocognitive disorders (albeit milder forms) compared to uninfected populations. PLWH who are immunosuppressed have a higher proportion of hemorrhagic strokes and strokes caused by opportunistic infection and HIV vasculopathy, while PLWH on long-term cART have higher rates of ischemic strokes, compared to HIV-seronegative controls. Brain large artery atherosclerosis in PLWH is associated with lower CD4 nadir and higher CD4 count during the stroke event. HIV vasculopathy, a form of non-atherosclerotic outward remodeling, on the other hand, is associated with protracted immunosuppression. HIV vasculopathy was also linked to a thinner media layer and increased adventitial macrophages, suggestive of non-atherosclerotic degeneration of the brain arterial wall in the setting of chronic central nervous system inflammation. Cerebrovascular architecture seems to be differentially affected by HIV infection in successfully treated versus immunosuppressed PLWH. Brain large artery atherosclerosis is prevalent even with long-term immune reconstitution post-cART. HIV-associated changes in brain arterial walls may also relate to higher rates of HIV-associated neurocognitive disorders, although milder forms are more prevalent in the post-cART era. The underlying mechanisms of HIV-associated pathological arterial remodeling remain poorly understood, but a role has been proposed for chronic HIV-associated inflammation with increased burden on the vasculature. Neuroimaging may come to play a role in assessing brain arterial remodeling and stratifying cerebrovascular risk, but the data remains inconclusive. An improved understanding of the different phenotypes of brain arterial remodeling associated with HIV may reveal opportunities to reduce rates of cerebrovascular disease in the aging population of PLWH on cART.
Impact of Left Atrial Appendage Morphology on Recurrence in Embolic Stroke of Undetermined Source and Atrial Cardiopathy
Authors: Gwak Dong-Seok , Choi WooChan , Kim Yong-Won , Kim Yong-Sun , Hwang Yang-Ha
Published: June 2021

Background: The left atrial appendage (LAA) is a major source of thrombus and non-chicken wing (CW). LAA morphology is a risk factor for embolic events in atrial fibrillation. However, the association of non-CW morphology with embolic stroke recurrence is unknown in patients with embolic stroke of undetermined source (ESUS) and atrial cardiopathy.

Methods: We conducted retrospective analyses using a prospective institutional stroke registry (2013–2017). Patients with ESUS and atrial cardiopathy were enrolled. Atrial cardiopathy was diagnosed if an increased left atrial diameter (>40 mm, men; >38 mm, women), supraventricular tachycardia, or LAA filling defect on computed tomography (CT) were present. Patients admitted >24 h after onset were excluded. LAA morphology was evaluated using CT and categorized into CW vs. non-CW types. The primary outcome was embolic stroke recurrence. Multivariable Cox proportional hazards models were used to examine the independent association between LAA morphology and outcome.

Results: Of 157 patients, 81 (51.6%) had CW LAA morphology. The median follow-up was 41.5 (interquartile range 12.3–58.5) months corresponding to 509.8 patient years. In total, 18 participants experienced embolic stroke recurrences (3.80 per 100 patient-years). Non-CW morphology was more associated with embolic stroke recurrence than CW morphology (hazard ratio (HR), 3.17; 95% confidence interval (CI), 1.13–8.91; p = 0.029). After adjusting for CHA2DS2-VASc score and number of potential embolic sources, non-CW morphology showed an independent association with outcome (adjusted HR, 2.90; 95% CI, 1.02–8.23; p = 0.045).

Conclusions: The LAA morphology types may help identify high risk of embolic stroke recurrence in ESUS with atrial cardiopathy. LAA morphology in atrial cardiopathy may provide clues for developing therapies tailored to specific mechanisms.
Degree and Complexity of Non-conscious Emotional Information Processing – A Review of Masked Priming Studies
Authors: Rohr Michaela , Wentura Dirk
Published: June 2021

Whether and to what degree information can be processed non-consciously has been a matter of debate since the emergence of psychology as a science. Emotional information, in particular, has often been assumed to have a privileged status because of its relevance for well-being and survival (e.g., to detect a threat). Indeed, many studies have explored non-conscious processing of evaluative (i.e., “emotional” in a broad sense) or emotional (e.g., facial expressions) features using the “silver bullet” of non-consciousness research – the masked sequential priming paradigm. In its prototypical form, this paradigm involves the categorization of target stimuli according to valence (e.g., is the target positive or negative?). Each target is preceded by a briefly presented prime that is followed by a mask to constrain awareness. Non-conscious processing is inferred from subtle influences of the prime on target processing, that is, whether responses are faster if prime and target are valence-congruent or not. We will review this research with a focus on three questions: first, which methods are used in this area to establish non-conscious processing? Second, is there evidence for non-conscious extraction of evaluative information? Third, is there evidence for non-conscious processing beyond a simple valence (positive/negative) discrimination, for example, processing of emotion-specific information? We will highlight important current debates and potential directions in which the field will move in the future.
Effect of Unilateral Acoustic Trauma on Neuronal Firing Activity in the Inferior Colliculus of Mice
Authors: Hsiao Chun-Jen , Galazyuk Alexander V.
Published: June 2021

Neural hyperactivity induced by sound exposure often correlates with the development of hyperacusis and/or tinnitus. In laboratory animals, hyperactivity is typically induced by unilateral sound exposure to preserve one ear for further testing of hearing performance. Most ascending fibers in the auditory system cross into the superior olivary complex and then ascend contralaterally. Therefore, unilateral exposure should be expected to mostly affect the contralateral side above the auditory brain stem. On the other hand, it is well known that a significant number of neurons have crossing fibers at every level of the auditory pathway, which may spread the effect of unilateral exposure onto the ipsilateral side. Here we demonstrate that unilateral sound exposure causes development of hyperactivity in both the contra and ipsilateral inferior colliculus in mice. We found that both the spontaneous firing rate and bursting activity were increased significantly compared to unexposed mice. The neurons with characteristic frequencies at or above the center frequency of exposure showed the greatest increase. Surprisingly, this increase was more pronounced in the ipsilateral inferior colliculus. This study highlights the importance of considering both ipsi- and contralateral effects in future studies utilizing unilateral sound exposure.
School-Based Surveillance of Respiratory Pathogens on “High-Touch” Surfaces
Authors: Murray Alastair F. , Emanuels Anne , Wolf Caitlin , Franko Nicholas , Starita Lea , Englund Janet A. , Chu Helen Y.
Published: June 2021

In order to assess the presence of respiratory pathogens on “high-touch” surfaces and inform sanitation practices at schools, pre-selected surfaces in elementary schools in Seattle, WA, USA were sampled weekly and tested by RT-PCR for 25 viral respiratory pathogens (including SARS-CoV-2 retrospectively) and S. pneumoniae during 2019–2020 winter respiratory illness season. Viral pathogens (rhinovirus, adenovirus, influenza) known to cause respiratory illness were detected on commonly touched surfaces, especially wooden surfaces, and matched the patterns of circulating virus in the community.
Case Report: Unexplained Fever and Chest Pain in a 5-Year-Old
Authors: Lubocka Paulina , Sabiniewicz Robert
Published: June 2021

Pericarditis is a rare, but severe cause of chest pain in children that can easily be overlooked during routine diagnostics. Fibrinous pericarditis was recognized in a 5-year old patient who presented with fever and chest pain. Despite thorough diagnostics, no evident etiological factor was found. Furthermore, the disease was unresponsive to broad-spectrum antibiotics and NSAIDs, however oral prednisone was found to cause rapid improvement in the patients' condition. It was presumed that the patient's condition was caused by a blunt trauma to the chest experienced 3 days prior to the onset of symptoms. In an 8-month follow-up the patient remains in good overall condition and no recurrences were observed.
The Field-Frequency Lock for Fast Field Cycling Magnetic Resonance: From NMR to MRI
Authors: Galuppini G. , Magni L. , Ferrante G.
Published: June 2021

Magnetic field stability plays a fundamental role in Nuclear Magnetic Resonance (NMR) and Magnetic Resonance Imaging (MRI) experiments, guaranteeing accuracy and reproducibility of results. While high levels of stabilization can be achieved for standard NMR techniques, this task becomes particularly challenging for Fast Field Cycling (FFC) NMR and MRI, where the main magnetic field is switched to higher or lower levels during the pulse sequence, and field stabilization must be guaranteed within a very short time after switching. Recent works have addressed the problem with rigorous tools from control system theory, proposing a model based approach for the synthesis of magnetic field controllers for FFC-NMR. While an experimental proof of concept has underlined the correctness of the approach for a complete FFC-NMR setup, the application of the novel, model based Field-Frequency Lock (FFL) system to a FFC-MRI scanner requires proper handling of field encoding gradients. Furthermore, the proof of concept work has also stressed how further advances in the hardware and firmware could improve the overall performances of the magnetic field control loop. The main aim of this perspective paper is then discussing the key challenges that arise in the development of the FFL system suitable for a complete MRI scanner, as well as defining possible research directions by means of preliminary, simulated experiments, with the final goal of favoring the development of a novel, model based FFL system for FFC-MRI.
Function of Chick Subcutaneous Adipose Tissue During the Embryonic and Posthatch Period
Authors: Zhao Haidong , Wu Mingli , Tang Xiaoqin , Li Qi , Yi Xiaohua , Wang Shuhui , Jia Cunling , Wei Zehui , Sun Xiuzhu
Published: June 2021

Since excess abdominal fat is one of the main problems in the broiler industry for the development of modern broiler and layer industry, the importance of subcutaneous adipose tissue has been neglected. However, chick subcutaneous adipose tissue appeared earlier than abdominal adipose tissue and more than abdominal adipose tissue. Despite a wealth of data, detailed information is lacking about the development and function of chick subcutaneous adipose tissue during the embryonic and posthatch period. Therefore, the objective of the current study was to determine the developmental changes of adipocyte differentiation, lipid synthesis, lipolysis, fatty acid β-oxidation, and lipid contents from E12 to D9.5. The results showed that subcutaneous adipose tissue was another important energy supply tissue during the posthatch period. In this stage, the mitochondrial copy number and fatty acid β-oxidation level significantly increased. It revealed that chick subcutaneous adipose tissue not only has the function of energy supply by lipidolysis but also performs the same function as brown adipose tissue to some extent, despite that the brown adipose tissue does not exist in birds. In addition, this finding improved the theory of energy supply in the embryonic and posthatch period and might provide theoretical basis on physiological characteristics of lipid metabolism in chicks.
Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)
Authors: Fang Zhi-Zhen , Lin-Wang Kui , Zhou Dan-Rong , Lin Yan-Juan , Jiang Cui-Cui , Pan Shao-Lin , Espley Richard V. , Andre Christelle M. , Ye Xin-Fu
Published: June 2021

Plum is one of the most important stone fruits in the world and anthocyanin-rich plums are increasingly popular due to their health-promoting potential. In this study, we investigated the mechanisms of anthocyanin accumulation in the flesh of the red-fleshed mutant of the yellow-fleshed plum ‘Sanyueli’. RNA-Seq and qRT-PCR showed that anthocyanin biosynthetic genes and the transcription factor PsMYB10.2 were upregulated in the flesh of the mutant. Functional testing in tobacco leaves indicated that PsMYB10.2 was an anthocyanin pathway activator and can activate the promoter of the anthocyanin biosynthetic genes PsUFGT and PsGST. The role of PsMYB10.2 in anthocyanin accumulation in the flesh of plum was further confirmed by virus-induced gene silencing. These results provide information for further elucidating the underlying mechanisms of anthocyanin accumulation in the flesh of plum and for the breeding of new red-fleshed plum cultivars.
Nitrogen and Phosphorus Signaling and Transport During Legume–Rhizobium Symbiosis
Authors: Ma Yanlin , Chen Rujin
Published: June 2021

Nitrogen (N) and phosphorus (P) are the two predominant mineral elements, which are not only essential for plant growth and development in general but also play a key role in symbiotic N fixation in legumes. Legume plants have evolved complex signaling networks to respond to both external and internal levels of these macronutrients to optimize symbiotic N fixation in nodules. Inorganic phosphate (Pi) and nitrate (NO3−) are the two major forms of P and N elements utilized by plants, respectively. Pi starvation and NO3− application both reduce symbiotic N fixation via similar changes in the nodule gene expression and invoke local and long-distance, systemic responses, of which N-compound feedback regulation of rhizobial nitrogenase activity appears to operate under both conditions. Most of the N and P signaling and transport processes have been investigated in model organisms, such as Medicago truncatula, Lotus japonicus, Glycine max, Phaseolus vulgaris, Arabidopsis thaliana, Oryza sativa, etc. We attempted to discuss some of these processes wherever appropriate, to serve as references for a better understanding of the N and P signaling and transport during symbiosis.
Federal Institutions and Strategic Policy Responses to COVID-19 Pandemic
Authors: Shvetsova Olga , VanDusky-Allen Julie , Zhirnov Andrei , Adeel Abdul Basit , Catalano Michael , Catalano Olivia , Giannelli Frank , Muftuoglu Ezgi , Rosenberg Dina , Sezgin Mehmet Halit , Zhao Tianyi
Published: June 2021

This essay examines the policy response of the federal and regional governments in federations to the COVID-19 crisis. We theorize that the COVID-19 policy response in federations is an outcome of strategic interaction among the federal and regional incumbents in the shadow of their varying accountability for health and the repercussions from the disruptive consequences of public health measures. Using the data from the COVID-19 Public Health Protective Policy Index Project, we study how the variables suggested by our theory correlate with the overall stringency of public health measures in federations as well as the contribution of the federal government to the making of these policies. Our results suggest that the public health measures taken in federations are at least as stringent as those in non-federations, and there is a cluster of federations on which a bulk of crisis policy making is carried by subnational governments. We find that the contribution of the federal government is, on average, higher in parliamentary systems; it appears to decline with the proximity of the next election in presidential republics, and to increase with the fragmentation of the legislative party system in parliamentary systems. Our analysis also suggests that when the federal government carries a significant share of responsibility for healthcare provision, it also tends to play a higher role in taking non-medical steps in response to the pandemic.
Perception of Faces and Elaboration of Gender and Victim/Aggressor Stereotypes: The Influence of Internet Use and of the Perceiver’s Personality
Authors: Bracci Margherita , Guidi Stefano , Marchigiani Enrica , Masini Maurizio , Palmitesta Paola , Parlangeli Oronzo
Published: June 2021

The use of social media, particularly among youngsters, is characterized by simple and fast image exploration, mostly of people, particularly faces. The study presented here was conducted in order to investigate stereotypical judgments about men and women concerning past events of aggression—perpetrated or suffered—expressed on the basis of their faces, and gender-related differences in the judgments. To this aim, 185 participants answered a structured questionnaire online. The questionnaire contained 30 photos of young people’s faces, 15 men and 15 women (Ma et al., 2015), selected on the basis of the neutrality of their expression, and participants were asked to rate each face with respect to masculinity/femininity, strength/weakness, and having a past of aggression, as a victim or as a perpetrator. Information about the empathic abilities and personality traits of participants were also collected. The results indicate that the stereotypes—both of gender and those of victims and perpetrators—emerge as a consequence of the visual exploration of faces that present no facial emotion. Some characteristics of the personality of the observers, such as neuroticism, extraversion, openness, conscientiousness, and affective empathy, have a role in facilitating or hindering stereotype processing, in different ways for male and female faces by male and female observers. In particular, both genders attribute their positive stereotypical attributes to same-gender faces: men see male faces as stronger, masculine, and more aggressive than women do, and women see female faces as more feminine, less weak, and less as victims than men do. Intensive use of social media emerges as a factor that could facilitate the expression of some stereotypes of violent experiences and considering female subjects as more aggressive. Findings in this study can contribute to research on aggressive behavior on the Internet and improve our understanding of the multiple factors involved in the elaboration of gender stereotypes relative to violent or victim behavior.
Personalized Nutrition Advice: Preferred Ways of Receiving Information Related to Psychological Characteristics
Authors: Dijksterhuis Garmt B. , Bouwman Emily P. , Taufik Danny
Published: June 2021

The primary goal of this study is to be able to discern specific types of consumers in terms of their psychosocial characteristics who may need different ways of receiving dietary advice. Knowing these types will enable a better fit of advice to consumers’ psychosocial characteristics, hereby stimulating healthy eating as the probability of compliance to the advice can potentially increase. The study draws upon several psychological theories to distinguish unique underlying factors that can subsequently be used to personalize nutrition information for consumers. A number of general psychological scales (self-regulation, action and coping self-efficacy, social comparison, intrinsic motivation, health info processing, need for cognition and for affect, and regulatory focus) are filled out by 988 respondents, including their preferences for receiving personalized forms of nutrition advice. The set of joint items from various psychological constructs is analyzed using a Principal Component Analysis to find underlying psychological characteristics. The PCA produces four components (explaining 51% of variation), that could be interpreted as ‘intrinsic interest and capabilities for healthy eating,’ ‘perceived difficulty to eat healthily,’ ‘self-worth insecurity,’ and ‘seeking positive challenges,’ respectively. By means of a Logistic Regression these components are able to predict preferences for different forms of receiving nutrition advice. This first component shows that a mind set for maintaining a healthy diet goes together with an interest in receiving an advice on what do to and on how that will affect one’s health. The second component predicts a preference for a fixed moment to receive information/advice. This may be a strategy of those that perceive difficulties to eat healthily, to help them control their healthy food intake. The insecurity that the third component models seems to lead to a wish for receiving specific advice about their health situation at fixed moments in time. The fourth component is a small component, therefore its prediction of a wish for an advice focussing on prevention of negative consequences is probably not a strong result. The study does point out that there appear different psychosocial types of consumers, that may benefit by being addressed according to their preference for receiving nutrition advice on specific moments, of a specific level of detail or pointing at the type of consequences the advice has. A better fit of the advice to the psychosocial characteristics of the recipient, captured in the identified components in the current study, may lead to an increase in compliance, although that will have to be further investigated in subsequent work.
How Do Video Games Elicit Guilt in Players? Linking Character Morality to Guilt Through a Mediation Analysis
Authors: Ahn Changhyun , Grizzard Matthew , Lee Seyoung
Published: June 2021

Research has consistently found that committing immoral actions in video games is capable of eliciting feelings of guilt in players. This study aimed to investigate the mediating role of theoretically-relevant psychological mechanisms: Perceived morality of the player-controlled character and self-attribution of virtual behavior. Based in psychological and communication theory, we derived a model that links these variables to character portrayal and guilt. A between-subjects experiment manipulated the portrayal of the player-controlled character (immoral vs. moral) and measured the mediating variables and self-reported guilt. The hypothesized model was tested using a path model. Data were generally consistent with hypotheses. Controlling an immoral character reduced perceived character morality. Perceived character morality positively predicted self-attribution of character behavior and negatively predicted guilt. Self-attribution positively predicted guilt but self-attribution and perceived character morality did not interact. Our findings suggest novel directions for continued research into how game features elicit emotional responses in players.
Do Implicit Theories About Ability Predict Self-Reports and Behavior-Proximal Measures of Primary School Students’ In-Class Cognitive and Metacognitive Learning Strategy Use?
Authors: Matthes Benjamin , Stoeger Heidrun
Published: June 2021

Although studies show relations between implicit theories about ability (ITs) and cognitive as well as metacognitive learning strategy use, existing studies suffer from an overreliance on broad-brush self-report measures of strategy use and limited ecological validity. Moreover, studies rarely examine younger students, and research on ITs and how much students benefit from interventions on learning strategies is lacking. Therefore, we investigated in ecologically valid settings (regular classroom instruction) whether primary school students’ ITs are related to their use of cognitive strategies (text reduction strategies based on identifying a text’s main ideas) and metacognitive strategies, assessed with (a) typical self-report scales and (b) more behavior-proximal measures. We also investigated whether students’ ITs predict how much they benefit from a previously evaluated 4-week intervention on cognitive and metacognitive strategies during regular classroom instruction (i.e., how much self-report scales and behavior-proximal measures for strategy use increase over the course of the intervention). Participants were 436 German primary school students (third and fourth graders). The data were analyzed using mixed linear regression analyses. Strength of students’ incremental theory was positively related to metacognitive strategy use, but not cognitive strategy use, when measured with self-report scales. For behavior-proximal measures, strength of incremental theory was positively related to the effectiveness of students’ cognitive strategy use and their extent of strategy monitoring (one of the two metacognitive strategies examined), but not to the quality of their goal setting (the second metacognitive strategy). Unexpectedly, students with a stronger incremental theory did not benefit more from the intervention.
Yoga Practice Is Beneficial for Maintaining Healthy Lifestyle and Endurance Under Restrictions and Stress Imposed by Lockdown During COVID-19 Pandemic
Authors: Nagarathna Raghuram , Anand Akshay , Rain Manjari , Srivastava Vinod , Sivapuram Madhava Sai , Kulkarni Ravi , Ilavarasu Judu , Sharma Manjunath N. K. , Singh Amit , Nagendra Hongasandra Ramarao
Published: June 2021

Uncertainty about Coronavirus disease 2019 (COVID-19) and resulting lockdown caused widespread panic, stress, and anxiety. Yoga is a known practice that reduces stress and anxiety and may enhance immunity. This study aimed to (1) investigate that including Yoga in daily routine is beneficial for physical and mental health, and (2) to evaluate lifestyle of Yoga practitioners that may be instrumental in coping with stress associated with lockdown. This is a pan-India cross-sectional survey study, which was conducted during the lockdown. A self-rated scale, COVID Health Assessment Scale (CHAS), was designed by 11 experts in 3 Delphi rounds (Content valid ratio = 0.85) to evaluate the physical health, mental health, lifestyle, and coping skills of the individuals. The survey was made available digitally using Google forms and collected 23,760 CHAS responses. There were 23,290 valid responses (98%). After the study's inclusion and exclusion criteria of yogic practices, the respondents were categorized into the Yoga (n = 9,840) and Non-Yoga (n = 3,377) groups, who actively practiced Yoga during the lockdown in India. The statistical analyses were performed running logistic and multinomial regression and calculating odds ratio estimation using R software version 4.0.0. The non-Yoga group was more likely to use substances and unhealthy food and less likely to have good quality sleep. Yoga practitioners reported good physical ability and endurance. Yoga group also showed less anxiety, stress, fear, and having better coping strategies than the non-Yoga group. The Yoga group displayed striking and superior ability to cope with stress and anxiety associated with lockdown and COVID-19. In the Yoga group, participants performing meditation reportedly had relatively better mental health. Yoga may lead to risk reduction of COVID-19 by decreasing stress and improving immunity if specific yoga protocols are implemented through a global public health initiative.
Yoga of Immortals Intervention Reduces Symptoms of Depression, Insomnia and Anxiety
Authors: Verma Sadhna , Donovan James , Tunuguntla Hari S. , Tunuguntla Renuka , Gupta Babu V. , Nandi Ayon , Shivanand Ishan
Published: June 2021

Background: Depression, anxiety, and disordered sleep are some common symptoms associated with sub-optimal mental health. During the COVID-19 pandemic, mental health issues have grown increasingly more prevalent in the population. Due to social distancing and other limitations during the pandemic, there is a need for home-based, flexible interventions that can improve mental health. The Yoga of Immortals (YOI) mobile application provides a structured intervention that can be used on any mobile device and applied from the user's home.

Methods: A total of 1,505 participants were enrolled in the study and used the YOI app for an 8-week period. Participants were asked to fill out three questionnaires: The Patient Health Questionnaire, 8 items (PHQ-8), the Generalized Anxiety Disorder questionnaire (GAD-7) and the Insomnia Severity Index (ISI). These three items were completed by 1,297 participants a total of four times: before starting YOI, two more times during use, and a fourth time after the 8-week usage period. Changes in PHQ8, GAD7 and ISI in participants were compared to a control group, who did not use the YOI app but completed all questionnaires (590 controls finished all questionnaires).

Results: Participants reported significant decreases in depression and anxiety-related symptoms. Compared to baseline, PHQ-8 scores decreased 50% on average after the 8-week period. GAD-7 scores also decreased by 40–50% on average, and ISI scores decreased by 50%. These changes were significantly greater (p < 0.05) than that observed in the control group. Participants who reported a previous diagnosis of depression and generalized anxiety reported significantly larger decreases in PHQ-8 and GAD-7 as compared to participants with no prior diagnosis (p < 0.05).

Conclusions: Regular use of the YOI intervention over an 8-week period led to significant decreases in symptoms of both depression and anxiety, as well as alleviation of insomnia.
Untargeted Proteomics-Based Approach to Investigate Unintended Changes in Genetically Modified Maize for Environmental Risk Assessment Purpose
Authors: Zanon Agapito-Tenfen Sarah , Guerra Miguel Pedro , Nodari Rubens Onofre , Wikmark Odd-Gunnar
Published: June 2021

Profiling technologies, such as proteomics, allow the simultaneous measurement and comparison of thousands of plant components without prior knowledge of their identity. The combination of these non-targeted methods facilitates a more comprehensive approach than targeted methods and thus provides additional opportunities to identify genotypic changes resulting from genetic modification, including new allergens or toxins. The purpose of this study was to investigate unintended changes in GM Bt maize grown in South Africa. In the present study, we used bi-dimensional gel electrophoresis based on fluorescence staining, coupled with mass spectrometry in order to compare the proteome of the field-grown transgenic hybrid (MON810) and its near-isogenic counterpart. Proteomic data showed that energy metabolism and redox homeostasis were unequally modulated in GM Bt and non-GM maize variety samples. In addition, a potential allergenic protein—pathogenesis related protein −1 has been identified in our sample set. Our data shows that the GM variety is not substantially equivalent to its non-transgenic near-isogenic variety and further studies should be conducted in order to address the biological relevance and the potential risks of such changes. These finding highlight the suitability of unbiased profiling approaches to complement current GMO risk assessment practices worldwide.
Does Small Ruminant Lentivirus Infection in Goats Predispose to Bacterial Infection of the Mammary Gland? A Preliminary Study
Authors: Urbańska Daria , Puchała Ryszard , Jarczak Justyna , Czopowicz Michał , Kaba Jarosław , Horbańczuk Karina , Bagnicka Emilia
Published: June 2021

The aim of this study was to determine whether asymptomatic small ruminant lentivirus seropositive (SRLV-SP) goats were more susceptible to bacterial infection of the udder when lactating by comparing the presence and species of pathogenic bacteria in their milk with the values for seronegative goats (SRLV-SN). Milk samples were collected during morning milking on days 20, 40, 60, 150, and 210 of lactation for three consecutive years and subjected to bacteriological examination. Staphylococcus caprae and S. xylosus were the most frequent strains identified in both SRLV-SP and SRLV-SN goats. The prevalence of pathogenic bacteria was the highest in the 1st lactation, regardless of SRLV status. Moreover, the prevalence of pathogenic bacteria was significantly higher in SRLV-SP goats, but only those in the 5th or further lactation (p = 0.010). This suggests a relationship between long-lasting SRLV infection and susceptibility to bacterial infections of the udder.
3D Volumetric Tensor Velocity Imaging with Low Computational Complexity Using a Row-Column Addressed Array
Authors: Chetverikova Kseniya , Jensen Jørgen Arendt , Traberg Marie Sand , Stuart Matthias Bo
Published: June 2021

A method for volumetric Tensor Velocity Imaging employing row-column (RC) addressed array with low computational complexity is investigated in simulations. An interleaved and non-interleaved sliding aperture sequence with 11 rows and 11 columns emissions by a 62 + 62 RC addressed array was used. The 3D velocities were estimated by a transverse oscillation (TO) cross-correlation estimator. Parabolic profiles at six different orientations corresponding to combinations of 0, 45 degrees azimuth angles and 90, 75, 60 beam-to-flow angles were investigated with 5 kHz pulse repetition frequencies. The Field II simulations were performed at a depth of 30 mm with peak velocity of 0.3 m/s. Across all vessel orientations, the relative mean bias varied from 2.3% to −14.26%, and the relative standard deviation varied from 0.43% to 5.5%. The best and worst performance was found at beam to flow angles of 90 degrees with 0 degrees rotation angle and 60 degrees beam-to-flow angle with 45 degrees rotation angle respectively. Due to the low channel count of the RC array and the low computational complexity, real-time implementation is feasible on conventional ultrasound systems.
Piezoresistive Characteristics of Nanocarbon Composite Strain Sensor by Its Longitudinal Pattern Design
Authors: Kim Sung-Yong , Choi Baek-Gyu , Oh Gwang-Won , Kim Chan-Jung , Jung Young-Seok , Jang Jin-Seok , Joung Kwan-Young , Suh Jun-Ho , Kang Inpil
Published: June 2021

For an engineering feasibility study, we studied a simple design to improve NCSS (nanocarbon composite strain sensor) sensitivity by using its geometric pattern at a macro scale. We fabricated bulk- and grid-type sensors with different filler content weights (wt.%) and different sensor lengths and investigated their sensitivity characteristics. We also proposed a unit gauge factor model of NCSS to find a correlation between sensor length and its sensitivity. NCSS sensitivity was improved proportional to its length incremental ratio and we were able to achieve better linear and consistent data from the grid type than the bulk type one. We conclude that the longer sensor length results in a larger change of resistance due to its piezoresistive unit summation and that sensor geometric pattern design is one of the important issues for axial load and deformation measurement.
Nanoencapsulation of Essential Oils as Natural Food Antimicrobial Agents: An Overview
Authors: Liao Wei , Badri Waisudin , Dumas Emilie , Ghnimi Sami , Elaissari Abdelhamid , Saurel Rémi , Gharsallaoui Adem
Published: June 2021

The global demand for safe and healthy food with minimal synthetic preservatives is continuously increasing. Natural food antimicrobials and especially essential oils (EOs) possess strong antimicrobial activities that could play a remarkable role as a novel source of food preservatives. Despite the excellent efficacy of EOs, they have not been widely used in the food industry due to some major intrinsic barriers, such as low water solubility, bioavailability, volatility, and stability in food systems. Recent advances in nanotechnology have the potential to address these existing barriers in order to use EOs as preservatives in food systems at low doses. Thus, in this review, we explored the latest advances of using natural actives as antimicrobial agents and the different strategies for nanoencapsulation used for this purpose. The state of the art concerning the antibacterial properties of EOs will be summarized, and the main latest applications of nanoencapsulated antimicrobial agents in food systems will be presented. This review should help researchers to better choose the most suitable encapsulation techniques and materials.
Prediction of Flow Velocity from the Flexural Vibration of a Fluid-Conveying Pipe Using the Transfer Function Method
Authors: Yang Wonseok
Published: June 2021

This study presents a method to predict the flow velocity in a fluid-conveying pipe using vibration signals from the pipe surface. The flexural vibration of a fluid pipe is investigated through wave propagation. The wavenumbers and mode shapes of the pipe are determined based on its mechanical properties and flow velocities. The transient components of wavenumbers at low frequencies vary and converge on all values at higher frequencies as the flow velocity is increased. While the stationary fluid pipe exhibits symmetrical mode shapes, pipes with increasing flow velocities exhibit an asymmetric mode shape distribution skewed on one side of the axis. The resonant frequencies shift to the low frequency side as the flow velocity increases. The analytical results of the vibration analysis are used in the transfer function method to predict the flow velocities. To validate the accuracy of the prediction method, numerical vibration signals simulated by the finite element model are used. The actual input flow velocity is compared with the numerical results regarding the same to gauge the accuracy of the prediction method. This method can be used to monitor the flow rate without using flow meters, and thus protect pipelines from sudden malfunction.
Epitaxial Graphene on 4H-SiC (0001) as a Versatile Platform for Materials Growth: Mini-Review
Authors: Shtepliuk Ivan , Giannazzo Filippo , Yakimova Rositsa
Published: June 2021

Material growth on a dangling-bond-free interface such as graphene is a challenging technological task, which usually requires additional surface pre-treatment steps (functionalization, seed layer formation) to provide enough reactive sites. Being one of the most promising and adaptable graphene-family materials, epitaxial graphene on SiC, due to its internal features (substrate-induced n-doping, compressive strain, terrace-stepped morphology, bilayer graphene nano-inclusions), may provide pre-conditions for the enhanced binding affinity of environmental species, precursor molecules, and metal atoms on the topmost graphene layer. It makes it possible to use untreated pristine epitaxial graphene as a versatile platform for the deposition of metals and insulators. This mini-review encompasses relevant aspects of magnetron sputtering and electrodeposition of selected metals (Au, Ag, Pb, Hg, Cu, Li) and atomic layer deposition of insulating Al2O3 layers on epitaxial graphene on 4H-SiC, focusing on understanding growth mechanisms. Special deliberation has been given to the effect of the deposited materials on the epitaxial graphene quality. The generalization of the experimental and theoretical results presented here is hopefully an important step towards new electronic devices (chemiresistors, Schottky diodes, field-effect transistors) for environmental sensing, nano-plasmonics, and biomedical applications.
Real-Time Volumetric Ultrasound Research Platform with 1024 Parallel Transmit and Receive Channels
Authors: Risser Christoph , Hewener Holger , Fournelle Marc , Fonfara Heinrich , Barry-Hummel Selina , Weber Steffen , Speicher Daniel , Tretbar Steffen
Published: June 2021

Volumetric ultrasound imaging is of great importance in many medical fields, especially in cardiology, but also in therapy monitoring applications. For development of new imaging technologies and scanning strategies, it is crucial to be able to use a hardware platform that is as free and flexible as possible and does not restrict the user in his research in any way. For this purpose, multi-channel ultrasound systems are particularly suitable, as they are able to control each individual element of a matrix array without the use of a multiplexer. We set out to develop a fully integrated, compact 1024-channel ultrasound system that provides full access to all transmission parameters and all digitized raw data of each transducer element. For this purpose, we synchronize four research scanners of our latest “DiPhAS” ultrasound research system generation, each with 256 parallel channels, all connected to a single PC on whose GPUs the entire signal processing is performed. All components of the system are housed in a compact, movable 19-inch rack. The system is designed as a general-purpose platform for research in volumetric imaging; however, the first-use case will be therapy monitoring by tracking radiation-sensitive ultrasound contrast agents.
The Optic Strut—CBCT Pneumatization Pattern and Prevalence
Authors: Sicoe Casian Teodor , Bichir Cătălina , Rusu Mugurel Constantin
Published: June 2021

(1) Background: The optic strut (OS) is a critical landmark for clinoid and paraclinoid surgical interventions. To our knowledge, the current literature only mentioned the OS as a possibility for a lesser sphenoidal wing (LSW) pneumatization path, without a proper study of the pneumatization patterns and prevalence within this structure. Thus, our aim was to fill in the missing information. (2) Methods: A retrospective study on 80 cone beam computed tomography (CBCT) files was conducted to assess the prevalence and the origins of pneumatization within the OS. (3) Results: The pneumatization patterns of the OS were: 56.25% from the sphenoid sinus, 1.25% from the posterior ethmoid air cells (PEAC), and 10% from Onodi cells (ONC). Simultaneous pneumatization of unique origin within the lesser sphenoidal wing (LSW) was found in 26.25% from the sphenoid sinus, 1.25% from PEAC, and 5% from ONC. Communication between both LSW roots through pneumatization was found in 6.25% of the files. (4) Conclusions: A careful radiological examination should precede clinical diagnosis and surgical interventions in the paraclinoid area to evaluate postoperative surgical risks and possible diffusion patterns for infection. Additionally, pneumatization within the OS alters its morphological features and thus, its utility as a landmark.
Adipose Tissue: Understanding the Heterogeneity of Stem Cells for Regenerative Medicine
Authors: Ong Wee Kiat , Chakraborty Smarajit , Sugii Shigeki
Published: June 2021

Adipose-derived stem cells (ASCs) have been increasingly used as a versatile source of mesenchymal stem cells (MSCs) for diverse clinical investigations. However, their applications often become complicated due to heterogeneity arising from various factors. Cellular heterogeneity can occur due to: (i) nomenclature and criteria for definition; (ii) adipose tissue depots (e.g., subcutaneous fat, visceral fat) from which ASCs are isolated; (iii) donor and inter-subject variation (age, body mass index, gender, and disease state); (iv) species difference; and (v) study design (in vivo versus in vitro) and tools used (e.g., antibody isolation and culture conditions). There are also actual differences in resident cell types that exhibit ASC/MSC characteristics. Multilineage-differentiating stress-enduring (Muse) cells and dedifferentiated fat (DFAT) cells have been reported as an alternative or derivative source of ASCs for application in regenerative medicine. In this review, we discuss these factors that contribute to the heterogeneity of human ASCs in detail, and what should be taken into consideration for overcoming challenges associated with such heterogeneity in the clinical use of ASCs. Attempts to understand, define, and standardize cellular heterogeneity are important in supporting therapeutic strategies and regulatory considerations for the use of ASCs.
Deformation Behavior of Elastomer-Glass Fiber-Reinforced Plastics in Dependence of Pneumatic Actuation
Authors: Mühlich Mona , González Edith A. , Born Larissa , Körner Axel , Schwill Lena , Gresser Götz T. , Knippers Jan
Published: June 2021

This paper aims to define the influencing design criteria for compliant folding mechanisms with pneumatically actuated hinges consisting of fiber-reinforced plastic (FRP). Through simulation and physical testing, the influence of stiffness, hinge width as well as variation of the stiffness, in the flaps without changing the stiffness in the hinge zone, was evaluated. Within a finite element model software, a workflow was developed for simulations, in order to infer mathematical models for the prediction of mechanical properties and the deformation behavior as a function of the aforementioned parameters. In conclusion, the bending angle increases with decreasing material stiffness and with increasing hinge width, while it is not affected by the flap stiffness itself. The defined workflow builds a basis for the development of a predictive model for the deformation behavior of FRPs.