bluuebunny/crossref_metadata_2025 huggingface dataset:
PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
Authors: Sant’Ana Rodrigo Ribeiro Arnt , Caprestano Clarissa Alves , Nodari Rubens Onofre , Agapito-Tenfen Sarah Zanon
Published: September 2020

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology allows the modification of DNA sequences in vivo at the location of interest. Although CRISPR-Cas9 can produce genomic changes that do not require DNA vector carriers, the use of transgenesis for the stable integration of DNA coding for gene-editing tools into plant genomes is still the most used approach. However, it can generate unintended transgenic integrations, while Cas9 prolonged-expression can increase cleavage at off-target sites. In addition, the selection of genetically modified cells from millions of treated ones, especially plant cells, is still challenging. In a protoplast system, previous studies claimed that such pitfalls would be averted by delivering pre-assembled ribonucleoprotein complexes (RNPs) composed of purified recombinant Cas9 enzyme and in vitro transcribed guide RNA (gRNA) molecules. We, therefore, aimed to develop the first DNA-free protocol for gene-editing in maize and introduced RNPs into their protoplasts with polyethylene glycol (PEG) 4000. We performed an effective transformation of maize protoplasts using different gRNAs sequences targeting the inositol phosphate kinase gene, and by applying two different exposure times to RNPs. Using a low-cost Sanger sequencing protocol, we observed an efficiency rate of 0.85 up to 5.85%, which is equivalent to DNA-free protocols used in other plant species. A positive correlation was displayed between the exposure time and mutation frequency. The mutation frequency was gRNA sequence- and exposure time-dependent. In the present study, we demonstrated that the suitability of RNP transfection was proven as an effective screening platform for gene-editing in maize. This efficient and relatively easy assay method for the selection of gRNA suitable for the editing of the gene of interest will be highly useful for genome editing in maize, since the genome size and GC-content are large and high in the maize genome, respectively. Nevertheless, the large amplitude of mutations at the target site require scrutiny when checking mutations at off-target sites and potential safety concerns.
Migration of Natural Hydrogen from Deep-Seated Sources in the São Francisco Basin, Brazil
Authors: Donzé Frédéric-Victor , Truche Laurent , Shekari Namin Parisa , Lefeuvre Nicolas , Bazarkina Elena F.
Published: September 2020

Hydrogen gas is seeping from the sedimentary basin of São Franciso, Brazil. The seepages of H2 are accompanied by helium, whose isotopes reveal a strong crustal signature. Geophysical data indicates that this intra-cratonic basin is characterized by (i) a relatively high geothermal gradient, (ii) deep faults delineating a horst and graben structure and affecting the entire sedimentary sequence, (iii) archean to paleoproterozoïc basements enriched in radiogenic elements and displaying mafic and ultramafic units, and (iv) a possible karstic reservoir located 400 m below the surface. The high geothermal gradient could be due to a thin lithosphere enriched in radiogenic elements, which can also contribute to a massive radiolysis process of water at depth, releasing a significant amount of H2. Alternatively, ultramafic rocks that may have generated H2 during their serpentinization are also documented in the basement. The seismic profiles show that the faults seen at the surface are deeply rooted in the basement, and can drain deep fluids to shallow depths in a short time scale. The carbonate reservoirs within the Bambuí group which forms the main part of the sedimentary layers, are crossed by the fault system and represent good candidates for temporary H2 accumulation zones. The formation by chemical dissolution of sinkholes located at 400 m depth might explain the presence of sub-circular depressions seen at the surface. These sinkholes might control the migration of gas from temporary storage reservoirs in the upper layer of the Bambuí formation to the surface. The fluxes of H2 escaping out of these structures, which have been recently documented, are discussed in light of the newly developed H2 production model in the Precambrian continental crust.
The Location of Settled Diasporas in Nova Scotian Fiction
Authors: Eguíbar-Holgado Miasol
Published: September 2020

This article offers a comparative study between two novels by Nova Scotian writers: George and Rue (2006), by George Elliott Clarke, and No Great Mischief (2000), by Alistair MacLeod. The main purpose of this analysis is to transform some of the pervasive assumptions that dominate interpretations of diasporic ontologies. Most conceptual contexts of diaspora, constructed around the idea of a homeland that is located elsewhere, can only partially be applied to historically long-established communities. Clarke’s and MacLeod’s works emphasize “native” identity, the historical presence of Africans and Scots in Nova Scotia and their ensuing attachment to the (home)land. The novels illustrate how the hostland may be transformed into a homeland after centuries of settlement. The favoring of routes over roots of many current conceptualizations of the diaspora thus contravenes the foundations on which these groups construct a “native/diasporic” identity. However, in settler colonies such as Canada, identifying these groups as unequivocally native would imply the displacement of the legitimate Indigenous populations of these territories. A direct transformation from diaspora to indigenous subjectivity would entail the obliteration of a (however distant) history of migration, on the one hand, and the disavowal of Indigenous groups, on the other. For these reasons, new vocabulary needs to be developed that accurately comes to terms with this experience, which I propose to refer to as “settled diaspora.” In settled diasporas, the notions of attachment to a local identity are reconciled with having distant points of origin. At the same time, there is conceptual room to accommodate claims of belonging that differ from those by Indigenous populations. Thus, the concept of the settled diaspora redresses critical restrictions in diaspora theory that prevent discourses of migration from being applied to spaces of settlement.
The Difficult Alliance between Vegan Parents and Pediatrician: A Case Report
Authors: Farella Ilaria , Panza Raffaella , Baldassarre Maria Elisabetta
Published: September 2020

The number of children on a vegetarian or vegan diet is gradually increasing. If not balanced and adequately supplemented, these dietary regimes can seriously impact the growth of children. Often the pediatrician is not perceived as a figure to rely on in the event of parents’ willingness to follow an alternative diet for their child. The feeling of distrust of parents towards the pediatrician can be dangerous for the health of the child. We present a 22-month-old boy with failure to thrive probably induced by an unbalanced vegetarian diet. The acquisition of the anamnestic data concerning the child’s diet was difficult because initially omitted by the parents. The poor compliance and the difficult follow-up highlights the difficulty in establishing a therapeutic alliance between parents who follow alternative regimens and the pediatrician.
Evaluation of Fecal Coliform Prevalence and Physicochemical Indicators in the Effluent from a Wastewater Treatment Plant in the North-West Province, South Africa
Authors: Makuwa Stenly , Tlou Matsobane , Fosso-Kankeu Elvis , Green Ezekiel
Published: September 2020

Compliance of the effluents from wastewater treatment plants (WWTPs) to the regulatory standards, which mostly entail the removal/reduction of organic waste and deactivation of the potential microbial pathogens is of great importance. The detection of indicator parameters can be used to determine the effectiveness of a WWTP and the level of compliance with the South African regulatory standards. The performance of the WWTP was assessed by biological, physical and chemical measures in wastewater final effluent. The Escherichia coli ranged from 0 and 2420 count/100 mL in the final effluent. The recorded values for the physicochemical parameters were within the following ranges: pH (7.03–8.49), electrical conductivity (81.63–126.5 mS/m), suspended solids (0.40–20.4 mg/L), ammonia (0–22.15 mg/L), Chemical Oxygen Demand (COD) (1–73 mg/L), nitrate (0–16.1 mg/L), ortho-phosphate (0–8.58 mg/L) and free chlorine (0–3.21 mg/L). Furthermore, the concentration of toxic heavy metals was recorded to be between 1–10 ug/L for arsenic, cadmium, lead and mercury. In conclusion, all the parameters that were evaluated in this study indicate that the studied WWTP is performing in accordance with the prescribed general limits.
Comparison of Ammonia Emission Estimation between Passive Sampler and Chamber System in Paddy Soil after Fertilizer Application
Authors: Kim Min-Suk , Koo Namin , Hyun Seunghun , Kim Jeong-Gyu
Published: September 2020

Ammonia (NH3) is an important precursor for particulate secondary aerosol formation. This study was conducted to evaluate the applicability of a passive sampler (PAS) for estimating the NH3 emission from chemical fertilizer application (85 kg-N·ha−1) at field scale and to compare the results with a chamber system for the calculation of NH3 emission flux at lab scale. The application of chemical fertilizer increased the ambient NH3 concentration from 7.11 to 16.87 μg·m−3. Also, the ambient NH3 concentration measured by the PAS was found to be highly influenced by not only the chemical fertilizer application but also the weather (temperature and rainfall). Wind rose diagram data can be useful for understanding the distribution of ambient NH3 concentration. In the case of a chamber with few environmental variables, NH3 was emitted very quickly in the early stages and gradually decreased, whereas it was delayed at intervals of about one week at the site. It was found that daily temperature range, atmospheric disturbance by wind and rainfall, changes in soil moisture, and the presence of a flooded water table were the main influencing factors. The PAS data and the chamber system data were observed to have significant differences in spatial-temporal scale. In order to reduce the gap, it seems to be necessary to further develop a chamber system, in order to improve the precision of field analysis and to strengthen the connection between experimental results.
Are the Water Quality Improvement Measures of China’s South-to-North Water Diversion Project Effective? A Case Study of Xuzhou Section in the East Route
Authors: Pan Ye , Yuan Yuan , Sun Ting , Wang Yuxin , Xie Yujing , Fan Zhengqiu
Published: September 2020

Xuzhou is the hub city of the east route of China’s South-to-North Water Diversion (SNWD) project and implemented dozens of measures to ensure the water quality security of the water transmission line. In order to detect the effectiveness of water quality improvement measures, the monthly water quality data of five water quality parameters from 2005 to 2015 of six state-controlled monitoring sites in Xuzhou section were selected for analysis. The results showed that the water quality improved from 2.95 in 2005 to 2.74 in 2015, as assessed by the comprehensive water quality identification index (CWQII), and basically reached the Class III standards of China’s Environmental Quality Standard for Surface Water (GB3838-2002) from 2011 to 2015. The trend analysis showed that the decline of ammonia nitrogen (NH3-N) was the most obvious among the five water quality parameters. However, the concentrations of phosphorus (TP) showed significant upward trends at three sites. The positive abrupt change of time series of water quality occurred in 2009–2011. The identification of influencing factors of water quality changes by multivariate statistical methods found that the urbanization factor accompanied by a decrease in agricultural nonpoint source pollution emissions and the enhancement of wastewater treatment capacity, the closure of factories with substandard emissions and precipitation were the major influencing factors of most water quality parameters, which confirmed the effectiveness of measures for water quality improvement in Xuzhou.
Different Movement Strategies in the Countermovement Jump Amongst a Large Cohort of NBA Players
Authors: Rauch Jacob , Leidersdorf Eric , Reeves Trent , Borkan Leah , Elliott Marcus , Ugrinowitsch Carlos
Published: September 2020

Previous research has demonstrated large amounts of inter-subject variability in downward (unweighting & braking) phase strategies in the countermovement jump (CMJ). The purpose of this study was to characterize downward phase strategies and associated temporal, kinematic and kinetic CMJ variables. One hundred and seventy-eight NBA (National Basketball Association) players (23.6 ± 3.7 years, 200.3 ± 8.0 cm; 99.4 ± 11.7 kg; CMJ height 68.7 ± 7.4 cm) performed three maximal CMJs. Force plate and 3D motion capture data were integrated to obtain kinematic and kinetic outputs. Afterwards, athletes were split into clusters based on downward phase characteristics (k-means cluster analysis). Lower limb joint angular displacement (i.e., delta flexion) explained the highest portion of point variability (89.3%), and three clusters were recommended (Ball Hall Index). Delta flexion was significantly different between clusters and players were characterized as “stiff flexors”, “hyper flexors”, or “hip flexors”. There were no significant differences in jump height between clusters (p > 0.05). Multiple regression analyses indicated that most of the jumping height variance was explained by the same four variables, (i.e., sum concentric relative force, knee extension velocity, knee extension acceleration, and height) regardless of the cluster (p < 0.05). However, each cluster had its own unique set of secondary predictor variables.
Biomarkers of Exposure among USA Adult Hookah Users: Results from Wave 1 of the Population Assessment of Tobacco and Health (PATH) Study (2013–2014)
Authors: Travers Mark J. , Rivard Cheryl , Sharma Eva , Retzky Sandra , Yucesoy Berran , Goniewicz Maciej L. , Stanton Cassandra A. , Chen Jiping , Callahan-Lyon Priscilla , Kimmel Heather L. , Xia Baoyun , Wang Yuesong , Sosnoff Connie S. , De Jesús Víctor R. , Blount Benjamin C. , Hecht Stephen S. , Hyland Andrew
Published: September 2020

Hookah smoking has become common in the USA, especially among young adults. This study measured biomarkers of exposure to known tobacco product toxicants in a population-based sample of exclusive, established hookah users. Urinary biomarker data from 1753 adults in Wave 1 of the Population Assessment of Tobacco and Health (PATH) Study were used to compare geometric mean concentrations of biomarkers of exposure in exclusive, established past 30-day hookah users to never users of tobacco. Geometric mean ratios were calculated comparing hookah user groups with never users adjusting for age, sex, race/ethnicity, education, past 30-day marijuana use, secondhand smoke exposure and creatinine. Past 30-day hookah users (n = 98) had 10.6 times the urinary cotinine level of never tobacco users. Compared to never tobacco users, past 30-day hookah users had 2.3 times the level of the carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), a metabolite of the tobacco-specific nitrosamine (TSNA) 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), 1.3 times higher polycyclic aromatic hydrocarbons (PAHs) 3-hydroxyfluorene and 1-hydroxypyrene, 1.8 times higher levels of acrylonitrile, 1.3 times higher levels of acrylamide, and 1.2 times higher levels of acrolein exposure. These data indicate that hookah use is a significant source of exposure to nicotine, carcinogens, and respiratory toxicants.
Arctigenin Attenuates Breast Cancer Progression through Decreasing GM-CSF/TSLP/STAT3/β-Catenin Signaling
Authors: Shi Hui , Zhao Luping , Guo Xinlin , Fang Runping , Zhang Hui , Dong Guanjun , Fu Jia , Yan Fenglian , Zhang Junfeng , Ning Zhaochen , Ma Qun , Li Zhihua , Li Chunxia , Dai Jun , Si Chuanping , Xiong Huabao
Published: September 2020

Invasive breast cancer is highly regulated by tumor-derived cytokines in tumor microenvironment. The development of drugs that specifically target cytokines are promising in breast cancer treatment. In this study, we reported that arctigenin, a bioactive compound from Arctium lappa L., could decrease tumor-promoting cytokines GM-CSF, MMP-3, MMP-9 and TSLP in breast cancer cells. Arctigenin not only inhibited the proliferation, but also the invasion and stemness of breast cancer cells via decreasing GM-CSF and TSLP. Mechanistically, arctigenin decreased the promoter activities of GM-CSF and TSLP via reducing the nuclear translocation of NF-κB p65 which is crucial for the transcription of GM-CSF and TSLP. Furthermore, arctigenin-induced depletion of GM-CSF and TSLP inhibited STAT3 phosphorylation and β-catenin signaling resulting in decreased proliferation, invasion and stemness of breast cancer cells in vitro and in vivo. Our findings provide new insights into the mechanism by which tumor-promoting cytokines regulate breast cancer progression and suggest that arctigenin is a promising candidate for cytokine-targeted breast cancer therapy.
Differential Impact of Calcitriol and Its Analogs on Tumor Stroma in Young and Aged Ovariectomized Mice Bearing 4T1 Mammary Gland Cancer
Authors: Anisiewicz Artur , Pawlik Agata , Filip-Psurska Beata , Wietrzyk Joanna
Published: September 2020

(1) Background: Vitamin D compounds (VDC) are extensively studied in the field of anticancer properties, including breast cancer. Previously, we showed that calcitriol and its analogs (PRI-2191 and PRI-2205) stimulate metastasis in 4T1 murine mammary gland cancer models in young mice, whereas the reverse effect was observed in aged ovariectomized (OVX) mice; (2) Methods: We determined the phenotype of monocytes/macrophages using FACS and examined the expression of selected genes and proteins by Real-Time PCR and ELISA; (3) Results: Activities of VDC are accompanied by an increase in the percentage of Ly6Clow anti-inflammatory monocytes in the spleen of young and a decrease in aged OVX mice. Treatment of young mice with VDC resulted in an increase of CCL2 plasma and tumor concentration and Arg1 in tumor. In later stage of tumor progression the expression of genes related to metastasis in lung tissue was decreased or increased, in old OVX or young mice, respectively; (4) Conclusions: Pro- or anti-metastatic effects of calcitriol and its analogs in young or aged OVX mice, respectively, can be attributed to the differences in the effects of VDC on the tumor microenvironment, as a consequence of differences in the immunity status of young and aged mice.
High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75
Authors: Zhang Wenli , Mese Kemal , Schellhorn Sebastian , Bahlmann Nora , Mach Nicolas , Bunz Oskar , Dhingra Akshay , Hage Elias , Lafon Marie-Edith , Wodrich Harald , Heim Albert , Ehrhardt Anja
Published: September 2020

Recently an increasing number of new adenovirus types associated with type-dependent pathogenicity have been identified. However, identification of these clinical isolates represents the very first step to characterize novel pathogens. For deeper analyses, these adenoviruses need to be further characterized in basic virology experiments or they could be applied in translational research. To achieve this goal, it is essential to get genetic access and to enable genetic modification of these novel adenovirus genomes (deletion, insertion, and mutation). Here we demonstrate a high-throughput approach to get genetic access to new adenoviruses via homologous recombination. We first defined the cloning conditions regarding homology arm-length and input adenoviral genome amounts. Then we cloned four naturally occurring adenoviruses (Ad70, Ad73, Ad74, and Ad75) into easy-to-manipulate plasmids and genetically modified them by reporter gene insertion. Three recombinant adenoviruses (Ad70, Ad73, and Ad74) containing a reporter cassette were successfully reconstituted. These novel reporter-labeled adenoviruses were further characterized using the inserted luciferase reporter with respect to receptor usage, presence of anti-adenovirus antibodies, and tropism in vitro. The identified receptor usage, the relatively low prevalence of anti-adenovirus antibodies, and the various cancer cell line transduction pattern are important features of these new pathogens providing essential information for their therapeutic application.
MBNL1-Associated Mitochondrial Dysfunction and Apoptosis in C2C12 Myotubes and Mouse Skeletal Muscle
Authors: Yokoyama Shingo , Ohno Yoshitaka , Egawa Tatsuro , Ohashi Kazuya , Ito Rika , Ortuste Quiroga Huascar Pedro , Yamashita Tomohiro , Goto Katsumasa
Published: September 2020

We explored the interrelationship between a tissue-specific alternative splicing factor muscleblind-like 1 (MBNL1) and peroxisome proliferator-activated receptor-γ coactivator 1-α (PGC-1α), B-cell lymphoma 2 (Bcl-2) or Bcl-2-associated X protein (Bax) in C2C12 myotubes and mouse skeletal muscle to investigate a possible physiological role of MBNL1 in mitochondrial-associated apoptosis of skeletal muscle. Expression level of PGC-1α and mitochondrial membrane potential evaluated by the fluorescence ratio of JC-1 aggregate to monomer in C2C12 myotubes were suppressed by knockdown of MBNL1. Conversely, the ratio of Bax to Bcl-2 as well as the apoptotic index in C2C12 myotubes was increased by MBNL1 knockdown. In plantaris muscle, on the other hand, not only the minimum muscle fiber diameter but also the expression level of MBNL1 and PGC-1α in of 100-week-old mice were significantly lower than that of 10-week-old mice. Furthermore, the ratio of Bax to Bcl-2 in mouse plantaris muscle was increased by aging. These results suggest that MBNL1 may play a key role in aging-associated muscle atrophy accompanied with mitochondrial dysfunction and apoptosis via mediating PGC-1α expression in skeletal muscle.
The Influence of Radiation on Bone and Bone Cells—Differential Effects on Osteoclasts and Osteoblasts
Authors: Donaubauer Anna-Jasmina , Deloch Lisa , Becker Ina , Fietkau Rainer , Frey Benjamin , Gaipl Udo S.
Published: September 2020

The bone is a complex organ that is dependent on a tight regulation between bone formation by osteoblasts (OBs) and bone resorption by osteoclasts (OCs). These processes can be influenced by environmental factors such as ionizing radiation (IR). In cancer therapy, IR is applied in high doses, leading to detrimental effects on bone, whereas radiation therapy with low doses of IR is applied for chronic degenerative and inflammatory diseases, with a positive impact especially on bone homeostasis. Moreover, the effects of IR are of particular interest in space travel, as astronauts suffer from bone loss due to space radiation and microgravity. This review summarizes the current state of knowledge on the effects of IR on bone with a special focus on the influence on OCs and OBs, as these cells are essential in bone remodeling. In addition, the influence of IR on the bone microenvironment is discussed. In summary, the effects of IR on bone and bone remodeling cells strongly depend on the applied radiation dose, as differential results are provided from in vivo as well as in vitro studies with varying doses of IR. Furthermore, the isolated effects of IR on a single cell type are difficult to determine, as the bone cells and bone microenvironment are building a tightly regulated network, influencing on one another. Therefore, future research is necessary in order to elucidate the influence of different bone cells on the overall radiation-induced effects on bone.
Cell Type-Specific Imaging of Calcium Signaling in Arabidopsis thaliana Seedling Roots Using GCaMP3
Authors: Krogman William , Sparks J. Alan , Blancaflor Elison B.
Published: September 2020

Cytoplasmic calcium ([Ca2+]cyt) is a well-characterized second messenger in eukaryotic cells. An elevation in [Ca2+]cyt levels is one of the earliest responses in plant cells after exposure to a range of environmental stimuli. Advances in understanding the role of [Ca2+]cyt in plant development has been facilitated by the use of genetically-encoded reporters such as GCaMP. Most of these studies have relied on promoters such as Cauliflower Mosaic Virus (35S) and Ubiquitin10 (UBQ10) to drive expression of GCaMP in all cell/tissue types. Plant organs such as roots consist of various cell types that likely exhibit unique [Ca2+]cyt responses to exogenous and endogenous signals. However, few studies have addressed this question. Here, we introduce a set of Arabidopsis thaliana lines expressing GCaMP3 in five root cell types including the columella, endodermis, cortex, epidermis, and trichoblasts. We found similarities and differences in the [Ca2+]cyt signature among these root cell types when exposed to adenosine tri-phosphate (ATP), glutamate, aluminum, and salt, which are known to trigger [Ca2+]cyt increases in root cells. These cell type-targeted GCaMP3 lines provide a new resource that should enable more in depth studies that address how a particular environmental stimulus is linked to specific root developmental pathways via [Ca2+]cyt.
Silencing PROK2 Inhibits Invasion of Human Cervical Cancer Cells by Targeting MMP15 Expression
Authors: Wu Min-Hua , Wu Pei-Ru , Hsieh Yi-Hsien , Lin Chia-Liang , Liu Chung-Jung , Ying Tsung-Ho
Published: September 2020

Cervical cancer is the second most frequent type of gynecologic cancer worldwide. Prokineticin 2 (PROK2) is reported to be involved in tumor progression in some malignant tumors. However, the role of PROK2 in the development of cervical cancer remains unknown. Our results indicate that PROK2 is overexpressed in the human cervical cancer. Cervical cancer patients with high PROK2 expression have a shorter overall survival rate (OS) and disease-free survival rate (DFS). PROK2 acts as a potential biomarker for predicting OS and DFS of cervical cancer patients. We further show that PROK2 is important factor for oncogenic migration and invasion in human cervical cancer cells. Knockdown PROK2 significantly inhibited cell migration, invasion, and MMP15 protein expression in HeLa cells. High expression of MMP15 is confirmed in the human cervical cancer, is significantly associated with the shorter overall survival rate (OS) and is correlated with PROK2 expression. Overexpression of PROK2 using PROK2 plasmid significantly reverses the function of knockdown PROK2, and further upregulates MMP15 expression, migration and invasion of human cervical cancer cells. In conclusion, our findings are the first to demonstrate the role of PROK2 as a novel and potential biomarker for clinical use, and reveal the oncogenic functions of PROK2 as therapeutic target for cervical cancer.
Beauveria bassiana Multifunction as an Endophyte: Growth Promotion and Biologic Control of Trialeurodes vaporariorum, (Westwood) (Hemiptera: Aleyrodidae) in Tomato
Authors: Barra-Bucarei Lorena , González Macarena Gerding , Iglesias Andrés France , Aguayo Gonzalo Silva , Peñalosa Matías Guerra , Vera Pedro Vergara
Published: September 2020

The tomato, Solanum lycopersicum L. is one of the most consumed vegetables in the world; nevertheless, it is affected by biotic and abiotic factors that reduce its productivity. The whitefly is globally considered as the main pest under protected crop conditions, where biologic control using endophytic fungi emerges as a sustainable alternative. We evaluated the indirect effects of five native endophytic strains of Beauveria bassiana on the reproduction of greenhouse whiteflies and the growth of tomatoes. The plant growth substrate was inoculated with five strains of this endophyte and the resulting plants were then exposed to whiteflies afterwards. The effect that endophytic strains had on phosphate solubilization, iron siderophore production, plant height, and plant biomass were evaluated. The evaluated endophytes reduced the number of eggs per cm2 on leaflets compared to the control and behaved similarly to the commercial synthetic insecticide. Leaflets inoculated with strains RGM-557, RGM-644 and RGM-731 showed fewer nymphs than the control and those treated with insecticide. RGM-557 and RGM-731 produced the greatest plant heights; RGM-731 obtained the greatest plant biomass. Our study provides evidence that native endophytic strains of B. bassiana have a biocontrol effect on whiteflies and could be used to promote tomato growth.
A Stepping Trail Making Test as an Indicator of Cognitive Impairment in Older Adults
Authors: Osuka Yosuke , Kim Hunkyung , Watanabe Yutaka , Taniguchi Yu , Kojima Narumi , Seino Satoshi , Kawai Hisashi , Sakurai Ryota , Inagaki Hiroki , Awata Shuichi , Shinkai Shoji
Published: September 2020

This study aimed to examine the concurrent validity of a novel motor-cognitive dual-task test, the Stepping Trail Making Test (S-TMT), as an indicator of cognitive impairment (CI), and compare its screening performance to that of motor or cognitive tests alone. This was a population-based cross-sectional study including 965 Japanese adults aged ≥ 70 years. To measure the time taken to perform the S-TMT, the participants were instructed to step on 16 numbers in sequence as quickly and accurately as possible. Motor and cognitive functions were assessed by gait speed and TMT part A (TMT-A), respectively. Participants were classified into CI (< 24 points), mild CI (MCI, 24–27 points), and intact cognition (> 27 points) categories based on their Mini-Mental State Examination score. Binary logistic regression models showed that the addition of the S-TMT to the covariates model gave the highest discrimination index (c-statistics), and significantly improved reclassification indices (net reclassification improvement and integrated discrimination improvement) for screening both CI and MCI compared to those of gait speed or TMT-A alone. These results show that S-TMT has a concurrent validity as a dual-task test for screening CI and MCI and better discrimination and reclassification performance than motor or cognitive tests alone in older adults.
Proton Conduction via Water Bridges Hydrated in the Collagen Film
Authors: Matsui Hiroshi , Matsuo Yasumitsu
Published: September 2020

Collagen films with proton conduction are a candidate of next generation of fuel-cell electrolyte. To clarify a relation between proton conductivity and formation of water networks in the collagen film originating from a tilapia’s scale, we systematically measured the ac conductivity, infrared absorption spectrum, and weight change as a function of relative humidity (RH) at room temperature. The integrated absorbance concerning an O–H stretching mode of water molecules increases above 60% RH in accordance with the weight change. The dc conductivity varies in the vicinity of 60 and 83% RH. From those results, we have determined the dc conductivity vs. hydration number (N) per unit (Gly-X-Y). The proton conduction is negligible in the collagen molecule itself, but dominated by the hydration shell, the development of which is characterized with three regions. For 0 < N < 2, the conductivity is extremely small, because the water molecule in the primary hydration shell has a little hydrogen bonded with each other. For 2 < N < 4, a quasi-one-dimensional proton conduction occurs through intra-water bridges in the helix. For 4 < N, the water molecule fills the helix, and inter-water bridges are formed in between the adjacent helices, so that a proton-conducting network is extended three dimensional.
Experimental and Numerical Investigation on the Transport Characteristics of Particle-Fluid Mixture in Y-Shaped Elbow
Authors: Hu Qiong , Zou Li , Lv Tong , Guan Yingjie , Sun Tiezhi
Published: September 2020

The Y-shaped elbow is used as a connecting pipe between the buffer and the lift pipe in the deep-sea mining system. After being mixed with seawater in the Y-shaped elbow, nodule particles are lifted to the sea surface mining ship via the lift pump. In this paper, we employ a computational fluid dynamics and discrete element coupled method (CFD-DEM) to study the characteristics of particle transport in the Y-shaped elbow. Considering a large diameter of the particles, we discuss the behavior of particles and fluid under different conveying velocities. In addition, the simulation was verified based on the experiment. The results show that the simulation agrees well with the experiment. On this basis, the distribution and motion characteristics of the particles in the Y-shaped elbow were obtained. The interaction between fluid and particles is also discussed. These findings suggest that the particles can be successfully transported when the pump runs at medium to high frequencies. The particles are basically moving along the pipe wall and slower than the fluid flow. Moreover, it was found that the particle motions are more complex with the increasing of conveying velocities, and it is closely related to the secondary flow of fluid. Some suggestions on the actual particle transportation can be put forward based on the research in this paper.
Spatial and Temporal Variability of Ice Algal Trophic Markers—With Recommendations about Their Application
Authors: Leu Eva , Brown Thomas A. , Graeve Martin , Wiktor Jozef , Hoppe Clara J. M. , Chierici Melissa , Fransson Agneta , Verbiest Sander , Kvernvik Ane C. , Greenacre Michael J.
Published: September 2020

Assessing the relative importance of sea ice algal-based production is often vital for studies about climate change impacts on Arctic marine ecosystems. Several types of lipid biomarkers and stable isotope ratios are widely used for tracing sea ic-associated (sympagic) vs. pelagic particulate organic matter (POM) in marine food webs. However, there has been limited understanding about the plasticity of these compounds in space and time, which constrains the robustness of some of those approaches. Furthermore, some of the markers are compromised by not being unambiguously specific for sea ice algae, whereas others might only be produced by a small sub-group of species. We analyzed fatty acids, highly branched isoprenoids (HBIs), stable isotope ratios of particulate organic carbon (POC) (δ13C), as well as δ13C of selected fatty acid markers during an Arctic sea ice algal bloom, focusing on spatial and temporal variability. We found remarkable differences between these approaches and show that inferences about bloom characteristics might even be contradictory between markers. The impact of environmental factors as causes of this considerable variability is highlighted and explained. We emphasize that awareness and, in some cases, caution is required when using lipid and stable isotope markers as tracers in food web studies and offer recommendations for the proper application of these valuable approaches.
The Formation Mechanism of a Self-Organized Periodic-Layered Structure at the Solid-(Cr, Fe)2B/Liquid-Al Interface
Authors: Wang Mengmeng , Ju Jiang , Li Jingjing , Zhou Yang , Lv Haiyang , Gao Haiyan , Wang Jun
Published: September 2020

A periodic-layered structure was observed in solid-(Cr, Fe)2B/liquid-Al diffusion couple at 750 °C. The interface morphology, the reaction products, and the potential formation mechanism of this periodic-layered structure were investigated using an electron probe microanalyzer (EPMA), scanning electron microscopy (SEM), electron backscatter diffraction (EBSD), transmission electron microscopy (TEM), and energy-dispersive spectroscopy (EDS). The results indicate that the reaction between (Cr, Fe)2B and liquid Al is a diffusion-controlled process. The formation of intermetallics involves both the superficial dissolution of Fe and Cr atoms and the inward diffusion of Al at the interface. The layered structure, as characterized by various experimental techniques, is alternated by a single FeAl3 layer and a (FeAl3 + Cr3AlB4) dual-phase layer. A potential mechanism describing the formation process of this periodic-layered structure was proposed based on the diffusion kinetics based on the experimental results.
Synthesis of Cryolite (Na3AlF6) from Secondary Aluminum Dross Generated in the Aluminum Recycling Process
Authors: Wan Bingbing , Li Wenfang , Sun Wanting , Liu Fangfang , Chen Bin , Xu Shiyao , Chen Weiping , Yi Aihua
Published: September 2020

Secondary aluminum dross (SAD) is regarded as a solid waste of aluminum recycling process that creates serious environmental and health concerns. However, SAD can also be used as a good source of aluminum, so that utilizing the SAD for the production of valuable products is a promising approach of recycling such waste. In the present work, a novel eco-friendly three-step process was proposed for the synthesis of cryolite (Na3AlF6) from the SAD, and it consisted of (1) water-washing pretreatment of SAD, (2) extraction of Al component via pyro-hydrometallurgy, including low-temperature alkaline smelting, water leaching and purification of leachate in sequence, (3) precipitation of cryolite from the purified NaAlO2 solution using the carbonation method. By analysis of the parameter optimization for each procedure, it was found that the maximum hydrolysis efficiency of aluminum nitride (AlN) in the SAD was around 68.3% accompanied with an extraction efficiency of Al reaching 91.5%. On this basis, the cryolite of high quality was synthesized under the following optimal carbonation conditions: reaction temperature of 75 °C, NaAlO2 concentration of 0.11 mol/L, F/(6Al) molar ratio of 1.10, and 99.99% CO2 gas pressure, and flow rate of 0.2 MPa and 0.5 L/min respectively. The formation of Na3AlF6 phase can be detected by XRD. The morphological feature observed by SEM revealed that the as-synthesized cryolite had a polyhedral shape (~1 μm size) with obvious agglomeration. The chemical composition and ignition loss of the as-synthesized cryolite complied well with the requirements of the Chinese national standard (GB/T 4291-2017).
Hybrid Polydopamine/Ag Shell-Encapsulated Magnetic Fe3O4 Nanosphere with High Antibacterial Activity
Authors: Fang Qunling , Xu Kezhu , Zhang Jianfeng , Xiong Qingshan , Duan Jinyu , Xuan Shouhu
Published: September 2020

The bacteria, which usually contaminate water environment, often cause terrible infectious diseases thus seriously threaten people’s health. To meet the increasing requirement of the public health care, an easily separable nanomaterial with sustainable anti-bacteria performance is required. This work reports a Fe3O4@PDA/Ag/PDA core-shell nanosphere in which the Ag nanocrystals immobilized on the magnetic carrier are protected by an external polydopamine (PDA) layer. The magnetic hybrid nanospheres are constructed by a tunable coating method and the particle parameters can be effectively controlled by the experimental condition. The antibacterial potential of the nanospheres is evaluable by using the Staphylococcus aureus and Escherichia coli as the models. The results indicate the Fe3O4@PDA/Ag/PDA core-shell nanospheres have a high antibacterial performance by measuring the minimum inhibitory concentration and the minimum bactericidal concentration. Finally, the product is expected to have a sustainable activity because the protecting PDA layer reduce the releasing rate of the Ag+ ions and the materials can be magnetically recovered from the media after the disinfection procedure.
Development of Unidirectional Cellular Structure with Multiple Pipe Layers and Characterisation of Its Mechanical Properties
Authors: Vesenjak Matej , Nakashima Yutaka , Hokamoto Kazuyuki , Ren Zoran , Marumo Yasuo
Published: September 2020

This study is concerned with the development of a new unidirectional cellular (UniPore) copper structure with multiple concentric pipe layers. The investigated UniPore structures were grouped into three main types, each having a different number of pipes (3, 4, and 5 pipes per transversal cross-section) and different pore arrangements. The specimens were fabricated by explosive compaction to achieve tightly compacted structures with a quasi-constant cross-section along the length of the specimens. The bonding between copper pipes was observed by a metallographic investigation, which showed that the pipes and bars were compressed tightly without voids. However, they were not welded together. The mechanical properties were determined by quasi-static compressive testing, where the typical behaviour for cellular materials was noted. The study showed that porosity significantly influences the mechanical properties, even more so than the arrangement of the pipes.
Hip Spacers with a Metal-on-Cement Articulation Did Not Show Significant Surface Alterations of the Metal Femoral Head in Two-Stage Revision for Periprosthetic Joint Infection
Authors: Lunz Andre , Sonntag Robert , Kretzer J. Philippe , Jaeger Sebastian , Bormann Therese , Streit Marcus R. , Beckmann Nicholas A. , Lehner Burkhard , Omlor Georg W.
Published: September 2020

Two-stage revision is considered the gold standard treatment for chronic periprosthetic joint infection (PJI). During the interim period, between explantation of the infected hip endoprosthesis and revision arthroplasty, individually formed articulating hip spacers made of polymethylmethacrylate (PMMA) bone cement can be used to provide better soft tissue preservation, local antibiotic release, and improved postoperative mobilization. If effective prevention from luxation is achieved, hip function and hence overall patient satisfaction is improved. Zirconium oxide particles inside conventional PMMA bone cement, however, are known to enhance third-body wear, which may cause alterations of the metal head in the articulating spacer and hence become a serious risk for the patient. Therefore, the aim of our study was to analyze whether the articular surface of cobalt-chrome (CoCr) femoral heads is significantly altered in the setting of a metal-on-cement articulation during the interim period of two-stage revision for PJI. We analyzed a consecutive series of 23 spacer cases and compared them with femoral heads from two series of conventional hip arthroplasty revisions with metal-on-polyethylene articulations and different time intervals in situ. To investigate metallic wear, the femoral heads were thoroughly examined, and their surface roughness was measured and analyzed. We found no significant differences between the two conventional hip arthroplasty groups, despite their very different times in situ. Furthermore, the individually different times in situ within the spacer group had no significant impact on surface roughness, either. Compared with the spacer group, the surface roughness of the metal femoral heads from both conventional hip arthroplasty groups were even higher. Within the spacer group, roughness parameters did not show significant differences regarding the five predefined locations on the metal head. We conclude that metal-on-cement articulations do not cause enhanced surface alterations of the metal femoral head and hence do not limit the application in articulating hip spacers in the setting of two-stage revision for PJI.
CO2 Curing Efficiency for Cement Paste and Mortars Produced by a Low Water-to-Cement Ratio
Authors: Han Seong Ho , Jun Yubin , Shin Tae Yong , Kim Jae Hong
Published: September 2020

Curing by CO2 is a way to utilize CO2 to reduce greenhouse gas emissions. Placing early-age cement paste in a CO2 chamber or pressure vessel accelerates its strength development. Cement carbonation is attributed to the quickened strength development, and CO2 uptake can be quantitatively evaluated by measuring CO2 gas pressure loss in the pressure vessel. A decrease in CO2 gas pressure is observed with all cement pastes and mortar samples regardless of the mix proportion and the casting method; one method involves compacting a low water-to-cement ratio mix, and the other method comprises a normal mix consolidated in a mold. The efficiency of the CO2 curing is superior when a 20% concentration of CO2 gas is supplied at a relative humidity of 75%. CO2 uptake in specimens with the same CO2 curing condition is different for each specimen size. As the specimen scale is larger, the depth of carbonation is smaller. Incorporating colloidal silica enhances the carbonation as well as the hydration of cement, which results in contributing to the increase in the 28-day strength.
Fabrication of Promising Antimicrobial Aloe Vera/PVA Electrospun Nanofibers for Protective Clothing
Authors: Khanzada Haleema , Salam Abdul , Qadir Muhammad Bilal , Phan Duy-Nam , Hassan Tufail , Munir Muhammad Usman , Pasha Khalid , Hassan Nafees , Khan Muhammad Qamar , Kim Ick Soo
Published: September 2020

In the present condition of COVID-19, the demand for antimicrobial products such as face masks and surgical gowns has increased. Because of this increasing demand, there is a need to conduct a study on the development of antimicrobial material. Therefore, this study was conducted on the development of Aloe Vera and Polyvinyl Alcohol (AV/PVA) electrospun nanofibers. Four different fibers were developed by varying the concentrations of Aloe vera (0.5%, 1.5%, 2.5%, and 3%) while maintaining the concentration of PVA constant. The developed samples were subjected to different characterization techniques such as SEM, FTIR, XRD, TGA, and ICP studies. After that, the antimicrobial activity of the developed Aloe Vera/PVA electrospun nanofibers was checked against Gram-positive (Staphylococcus aureus) bacteria and Gram-negative (Escherichia coli) bacteria. The developed nanofibers had high profile antibacterial activity against both bacteria, but showed excellent results against S. aureus bacteria as compared with E. coli. These nanofibers have potential applications in the development of surgical gowns, gloves, etc.
Second-Order Unconditionally Stable Direct Methods for Allen–Cahn and Conservative Allen–Cahn Equations on Surfaces
Authors: Xia Binhu , Li Yibao , Li Zhong
Published: September 2020

This paper describes temporally second-order unconditionally stable direct methods for Allen–Cahn and conservative Allen–Cahn equations on surfaces. The discretization is performed via a surface mesh consisting of piecewise triangles and its dual-surface polygonal tessellation. We prove that the proposed schemes, which combine a linearly stabilized splitting scheme, are unconditionally energy-stable. The resulting system of discrete equations is linear and is simple to implement. Several numerical experiments are performed to demonstrate the performance of our proposed algorithm.