bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimized femtosecond laser direct-written fiber Bragg gratings with high reflectivity and low loss
Authors: Wu Jiafeng , Xu Xizhen , Liao Changrui , Weng Xiaoyu , Liu Liwei , Qu Junle , Wang Yiping , He Jun
Published: January 2023

We propose and experimentally demonstrate a femtosecond laser plane-by-plane (Pl-b-Pl) technology for inscription of high-quality fiber Bragg gratings (FBGs). The spherical aberration (SA) was introduced to elongate the focal volume, and then combined with the scanning process, an expanded rectangular refractive index modification (RIM) region can be achieved. Such RIM regions exhibit a length of 15 µm and a width of 14 µm. Note that it consists of a negative region and a positive region. We have systematically studied the influence of the overlap between the RIM region and fiber core on the spectrum of FBG. After optimizing, the core of a conventional single-mode fiber (SMF) is covered completely by using the positive RIM region, resulting in a significant enhancement of the coupling strength coefficient (i.e., 3177.6 m-1). A 500 µm long FBG assembled by using these RIM regions can achieve a high reflectivity of 95.83%. Moreover, the cladding mode resonances in transmission spectrum are suppressed thoroughly, since the localized effect in RIM region was avoided. In addition, this FBG exhibits a high birefringence of 2.13 × 10−4. Therefore, the proposed fabrication method can be used to inscribe high-quality FBGs that could be used in many fields such as communication, fiber laser, polarization-selective filtering and multi-parameter sensing.
Visualized concentration sensors based on fluorescence indication in a dye-doped polymer microwire
Authors: Han Jinjin , Zheng Fangzhu , Chen Minghui , Geng Shihang , Zhang Qiongdan , Lin Zhili , Wu Zhijun , Pu Jixiong , Dai Hao , Zhang Xining
Published: January 2023

We demonstrate visualized microwire sensors based on fluorescence indication for detecting the concentrations of the aqueous solutions. The single Rhodamine (RhB) doped polymer microwires (PMWs) which are excited by the waveguiding excitation method are used as the sensory area. According to the fluorescent microimages of the PMWs, stable periodic oscillations could be observed in the RhB-doped PMWs. The fluorescent period which is dependent on the concentration is further analyzed by image processing and information extraction algorithms. Corresponding to a 1.0% change, the period length change of the visualized sensor reaches ∼380 nm, ∼270 nm, and ∼300 nm in NaCl, KCl, and sucrose solutions, respectively. The dection limits of the three solutions are estimated to be around 1.5 × 10−4%. The dye-doped PMW sensors by fluorescence indication and image analysis proposed here realize the direct visualized detection in concentration sensing, making it possible to avoid the challenges of stability and weak signal detection and offer a potentially stable and cost-effective approach for micro/nanofiber sensor application.
Photonic integrated circuit with multiple waveguide layers for broadband high-efficient 3D OPA
Authors: Wu Dachuan , Yu Bowen , Kakdarvishi Venus , Yi Yasha
Published: January 2023

The traditional photonic integrated circuit (PIC) inherits the mature CMOS fabrication process from the electronic integrated circuit (IC) industry. However, this process also limits the PIC structure to a single-waveguide-layer configuration. In this work, we explore the possibility of the multi-waveguide-layer PIC by proposing and demonstrating a 3D optical phased array (OPA) device, with the light exiting from the edge of the device, based on multi-layer Si3N4/SiO2 stacks. This device is in a multi-waveguide-layer configuration at every single position of the device. This configuration offers the possibility of using edge couplers at both the input and the emitting ends to achieve broadband high efficiency, and its uniqueness provides the potential for a more extended detection range in the lidar application. The device has been studied by numerical simulation, and proof-of-concept samples have been fabricated and tested.
Effectiveness of mRNA boosters after homologous primary series with BNT162b2 or ChAdOx1 against symptomatic infection and severe COVID-19 in Brazil and Scotland: A test-negative design case–control study
Authors: Cerqueira-Silva Thiago , Shah Syed Ahmar , Robertson Chris , Sanchez Mauro , Katikireddi Srinivasa Vittal , de Araujo Oliveira Vinicius , Paixão Enny S. , Rudan Igor , Junior Juracy Bertoldo , Penna Gerson O. , Pearce Neil , Werneck Guilherme Loureiro , Barreto Mauricio L. , Boaventura Viviane S. , Sheikh Aziz , Barral-Netto Manoel
Published: January 2023

Background

Brazil and Scotland have used mRNA boosters in their respective populations since September 2021, with Omicron’s emergence accelerating their booster program. Despite this, both countries have reported substantial recent increases in Coronavirus Disease 2019 (COVID-19) cases. The duration of the protection conferred by the booster dose against symptomatic Omicron cases and severe outcomes is unclear.

Methods and findings

Using a test-negative design, we analyzed national databases to estimate the vaccine effectiveness (VE) of a primary series (with ChAdOx1 or BNT162b2) plus an mRNA vaccine booster (with BNT162b2 or mRNA-1273) against symptomatic Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and severe COVID-19 outcomes (hospitalization or death) during the period of Omicron dominance in Brazil and Scotland compared to unvaccinated individuals. Additional analyses included stratification by age group (18 to 49, 50 to 64, ≥65). All individuals aged 18 years or older who reported acute respiratory illness symptoms and tested for SARS-CoV-2 infection between January 1, 2022, and April 23, 2022, in Brazil and Scotland were eligible for the study. At 14 to 29 days after the mRNA booster, the VE against symptomatic SARS-CoV-2 infection of ChAdOx1 plus BNT162b2 booster was 51.6%, (95% confidence interval (CI): [51.0, 52.2], p < 0.001) in Brazil and 67.1% (95% CI [65.5, 68.5], p < 0.001) in Scotland. At ≥4 months, protection against symptomatic infection waned to 4.2% (95% CI [0.7, 7.6], p = 0.02) in Brazil and 37.4% (95% CI [33.8, 40.9], p < 0.001) in Scotland. VE against severe outcomes in Brazil was 93.5% (95% CI [93.0, 94.0], p < 0.001) at 14 to 29 days post-booster, decreasing to 82.3% (95% CI [79.7, 84.7], p < 0.001) and 98.3% (95% CI [87.3, 99.8], p < 0.001) to 77.8% (95% CI [51.4, 89.9], p < 0.001) in Scotland for the same periods. Similar results were obtained with the primary series of BNT162b2 plus homologous booster. Potential limitations of this study were that we assumed that all cases included in the analysis were due to the Omicron variant based on the period of dominance and the limited follow-up time since the booster dose.

Conclusions

We observed that mRNA boosters after a primary vaccination course with either mRNA or viral-vector vaccines provided modest, short-lived protection against symptomatic infection with Omicron but substantial and more sustained protection against severe COVID-19 outcomes for at least 3 months.
Malnutrition-related parasite dissemination from the skin in visceral leishmaniasis is driven by PGE2-mediated amplification of CCR7-related trafficking of infected inflammatory monocytes
Authors: Osorio E. Yaneth , Uscanga-Palomeque Ashanti , Patterson Grace T. , Cordova Erika , Travi Bruno L. , Soong Lynn , Melby Peter C.
Published: January 2023

People are infected with Leishmania donovani when the parasite is deposited in the dermis during the blood meal of the sand fly vector. Most infected people develop a subclinical latent infection, but some develop progressive visceral leishmaniasis. Malnutrition is a risk factor for the development of active VL. We previously demonstrated increased parasite dissemination from the skin to visceral organs in a murine model of malnutrition. Here we investigated the mechanism of early parasite dissemination. After delivery of L. donovani to the skin, we found enhanced capture of parasites by inflammatory monocytes and neutrophils in the skin of malnourished mice. However, parasite dissemination in malnourished mice was driven primarily by infected inflammatory monocytes, which showed increased CCR7 expression, greater intrinsic migratory capacity, and increased trafficking from skin to spleen. PGE2 production, which was increased at the site of skin infection, increased monocyte CCR7 expression and promoted CCR7-related monocyte-mediated early parasite dissemination in malnourished mice. Parasite dissemination in monocytes was reduced by inhibition of PGE2, knockdown or silencing of CCR7 in monocytes, and depletion of inflammatory monocytes through administration of diphtheria toxin to CSFR1-DTR transgenic mice that have monocyte-specific DT receptor expression. CCR7-driven trafficking of infected inflammatory monocytes through the lymph node was accompanied by increased expression of its ligands CCL19 and CCL21. These results show that the CCR7/PGE2 axis is responsible for the increased trafficking of L. donovani-infected inflammatory monocytes from the skin to the spleen in the malnourished host. Undernutrition and production of PGE2 are potential targets to reduce the risk of people developing VL. Nutritional interventions that target improved immune function and reduced PGE2 synthesis should be studied in people at risk of developing VL.
MAGNET: A web-based application for gene set enrichment analysis using macrophage data sets
Authors: Chen Shang-Yang , Gadhvi Gaurav , Winter Deborah R.
Published: January 2023

Characterization of gene lists obtained from high-throughput genomic experiments is an essential task to uncover the underlying biological insights. A common strategy is to perform enrichment analyses that utilize standardized biological annotations, such as GO and KEGG pathways, which attempt to encompass all domains of biology. However, this approach provides generalized, static results that may fail to capture subtleties associated with research questions within a specific domain. Thus, there is a need for an application that can provide precise, relevant results by leveraging the latest research. We have therefore developed an interactive web application, Macrophage Annotation of Gene Network Enrichment Tool (MAGNET), for performing enrichment analyses on gene sets that are specifically relevant to macrophages. Using the hypergeometric distribution, MAGNET assesses the significance of overlapping genes with annotations that were curated from published manuscripts and data repositories. We implemented numerous features that enhance utility and user-friendliness, such as the simultaneous testing of multiple gene sets, different visualization options, option to upload custom datasets, and downloadable outputs. Here, we use three example studies compared against our current database of ten publications on mouse macrophages to demonstrate that MAGNET provides relevant and unique results that complement conventional enrichment analysis tools. Although specific to macrophage datasets, we envision MAGNET will catalyze developments of similar applications in other domains of interest. MAGNET can be freely accessed at the URL https://magnet-winterlab.herokuapp.com. Website implemented in Python and PostgreSQL, with all major browsers supported. The source code is available at https://github.com/sychen9584/MAGNET.
Gender differences in motor and non-motor symptoms in individuals with mild-moderate Parkinson’s disease
Authors: Abraham Amit , Bay Allison A. , Ni Liang , Schindler Nicole , Singh Eeshani , Leeth Ella , Bozorg Ariyana , Hart Ariel R. , Hackney Madeleine E.
Published: January 2023

Background

Parkinson’s disease (PD) affects both men and women with documented gender differences across functional domains, with findings varying among reports. Knowledge regarding gender differences in PD for different geographic locations is important for further understanding of the disease and for developing personalized gender-specific PD assessment tools and therapies.

Objective

This study aimed to examine gender differences in PD-related motor, motor-cognitive, cognitive, and psychosocial function in people with PD from the southern United States (US).

Methods

199 (127 men and 72 women;Mage: 69.08±8.94) individuals with mild-moderate idiopathic PD (Hoehn &Yahr (H&Y) Median = 2, stages I-III) from a large metro area in the southeastern US were included in this retrospective, cross-sectional study. Motor, motor-cognitive, cognitive, and psychosocial data were obtained using standardized and validated clinical tests. Univariate analyses were performed, adjusting for age and housing type.

Results

After adjustment for age, housing, PD duration and fall rate, men exhibited statistically significantly greater motor (Movement Disorders Society (MDS)-Unified Parkinson Disease Rating Scale (UPDRS)-II) and non-motor (MDS-UPDRS-I) impact of PD, and more severe motor signs (MDS-UPDRS-III). Men exhibited worse PD-specific health-related quality of life related to mobility, activities of daily living, emotional well-being, cognitive impairment, communication, and more depressive symptoms. Men performed worse on a subtraction working memory task. Women had slower fast gait speed.

Conclusions

In the southeastern United States, men may experience worse PD-related quality of life and more depression than women. Many non-motor and motor variables that are not PD specific show no differences between genders in this cohort. These findings can contribute to the development of gender-sensitive assessment and rehabilitation policies and protocols for people with PD.
Determinants of household dropout from community-based health insurance program in northwest Ethiopia; A community-based case-control study
Authors: Wassie Gizachew Tadesse , Tadesse Getasew , Nebeb Gebeyehu Tsega , Melese Amare Alemu , Ayalew Agumas Fentahun , Bantie Getasew Mulat
Published: January 2023

Background

Community-Based Health Insurance (CBHI) is an evolving program for delivering financial protection against the cost of illness and enhancing access to quality health services for low-income informal households.

Objective

The study aimed to identify determinants of household dropout from a CBHI program in Mecha district, North West Ethiopia, 2019.

Methods

A community-based case-control study was conducted in the Mecha district from March 10 to June 10, 2018. The final sample was 634 (317 cases and 317 controls) determined by the two-population proportion formula, and these samples were selected using a multi-stage sampling technique. The collected data was entered into Epi-data version 3.1 and analyzed using R version 4.0 software. Descriptive statistics computed. A simple logistic analysis was run (at 95% CI and p-value < 0.05) to identify the determinants for the dropout from CBHI.

Results

Poor perceived quality of care (AOR = 3.66; 95%CI: 2.35, 5.69), low knowledge of community-based health insurance (AOR = 6.02; 95%CI: 2.97, 12.26), no active community communication (AOR = 5.41; 95%CI: 3.29, 8.90) no chronic illness (AOR = 10.82; 95%CI: 5.52, 21.21) premium fee is not affordable (AOR = 2.35; 95%CI: 1.47, 3.77), and out of pocket money not reimbursed (AOR = 9.37; 95%CI: 4.44, 19.77) were the determinants for the dropout from CBHI.

Conclusions

Poor perceived quality of care, low knowledge of CBHI, no active community participation, no chronic illness, premium fees are not affordable, and out-of-pocket money not reimbursed were the determinants for the dropout from CBHI.
Pandemic news information uncertainty—News dynamics mirror differential response strategies to COVID-19
Authors: Nielbo Kristoffer , Enevoldsen Kenneth , Baglini Rebekah , Fano Elena , Roepstorff Andreas , Gao Jianbo
Published: January 2023

National differences in uncertainty, inequality, and trust have been accentuated by COVID-19. There are indications that the pandemic has impacted societies characterized by high uncertainty, inequality, and low trust harder than societies characterized by low uncertainty, equality, and high trust. This study investigates differential response strategies to COVID-19 as reflected in news media of two otherwise similar low uncertainty societies: Denmark and Sweden. The comparison is made using a recent approach to information dynamics in unstructured data. The main findings are that the news dynamics generally mirror public-health policies, capture fundamental socio-cultural variables related to uncertainty and trust, and may provide a measure of societal uncertainty. The findings can provide insights into evolutionary trajectories of decision-making under high uncertainty and, from a methodological level, be used to develop a media-based index of uncertainty and trust.
Sores of boreal moose reveal a previously unknown genetic lineage of parasitic nematode within the genus Onchocerca
Authors: Benedict Bridgett M. , Barboza Perry S. , Crouse John A. , Groch Katia R. , Kulpa Matthew R. , Thompson Dan P. , Verocai Guilherme G. , Wiener Dominique J.
Published: January 2023

Long-standing reports of open sores on the hind legs of moose (Alces alces) have been recorded in Alaska (as well as Canada, Europe, and Michigan), eliciting concerns about causes and infection. We used histological and genomic methods to investigate the sores from 20 adult moose on the Kenai Peninsula, Alaska. We paired this with thermal imagery and molt scoring of adult moose to further describe sore formation and understand its timing. Severe, ulcerative and eosinophilic dermatitis was found in all moose with sores present, and microfilariae within intraepidermal pustules were additionally found in four samples. Genetic analysis of sores from moose revealed a previously unknown genetic lineage of Onchocerca. Adult moose molt and lose their barrier of protection against flies in June and July during peak fly activity, leaving them vulnerable and allowing the development of sores. In summary, our results indicate that the cause for the sores on the hindleg of moose is a previously unknown genetic lineage of Onchocerca, probably transmitted by black flies, in timing with the molt cycle of adult moose. These sores leave moose exposed to pathogens, making them vulnerable, and challenging their health and fitness.
How causal machine learning can leverage marketing strategies: Assessing and improving the performance of a coupon campaign
Authors: Langen Henrika , Huber Martin
Published: January 2023

We apply causal machine learning algorithms to assess the causal effect of a marketing intervention, namely a coupon campaign, on the sales of a retailer. Besides assessing the average impacts of different types of coupons, we also investigate the heterogeneity of causal effects across different subgroups of customers, e.g., between clients with relatively high vs. low prior purchases. Finally, we use optimal policy learning to determine (in a data-driven way) which customer groups should be targeted by the coupon campaign in order to maximize the marketing intervention’s effectiveness in terms of sales. We find that only two out of the five coupon categories examined, namely coupons applicable to the product categories of drugstore items and other food, have a statistically significant positive effect on retailer sales. The assessment of group average treatment effects reveals substantial differences in the impact of coupon provision across customer groups, particularly across customer groups as defined by prior purchases at the store, with drugstore coupons being particularly effective among customers with high prior purchases and other food coupons among customers with low prior purchases. Our study provides a use case for the application of causal machine learning in business analytics to evaluate the causal impact of specific firm policies (like marketing campaigns) for decision support.
Quantifying fish range shifts across poorly defined management boundaries
Authors: Palacios-Abrantes Juliano , Crosson Scott , Dumas Chris , Fujita Rod , Levine Arielle , Longo Catherine , Jensen Olaf P.
Published: January 2023

Management regimes of marine resources that rely on spatial boundaries might be poorly adapted to climate change shifts in species distributions. This is of specific concern for the management of fish stocks that cross management jurisdictions, known as shared stocks. Transitioning to dynamic rules in spatial management has been suggested as a solution for mismatches between species distributions and the spatial boundaries. However, in many cases spatial boundaries are not clearly drawn, hampering such transitions. Here, we use black sea bass (Centropristis striata), summer flounder (Paralichthys dentatus) and scup (Stenotomus chrysops) as case studies to explore different approaches to designing spatial regulatory units to facilitate the adaptation of fisheries management to shifting distributions of shared stocks. First, we determine the yearly distribution of each stock within the United States Exclusive Economic Zone from 1951 to 2019 during Fall and Spring sampling seasons. Second, we explore two approaches for drawing regulatory units based on state waters and historical landings. Finally, we estimate each state’s proportion of the stock’s distribution and compare historical and recent values. We show that the distribution of all three stocks has changed relative to the years used to determine the current quota allocation across states, with an overall gain for central-northern states at the expense of the southernmost states. In terms of the distribution of allocation, we find that, while seasonal differences exist, the biggest differences in the proportion of the stock spatial distribution attributed to each state come from the method for designing regulatory units. Here, we show that the method used to define allocation units can have meaningful impacts on resulting adaptive policy. As climate change-driven conflicts in fishing resource allocation are expected to increase and deepen around the world, we provide a replicable approach to make an informed and transparent choice to support data-driven decision-making.
Expectations and attitudes towards medical artificial intelligence: A qualitative study in the field of stroke
Authors: Amann Julia , Vayena Effy , Ormond Kelly E. , Frey Dietmar , Madai Vince I. , Blasimme Alessandro
Published: January 2023

Introduction

Artificial intelligence (AI) has the potential to transform clinical decision-making as we know it. Powered by sophisticated machine learning algorithms, clinical decision support systems (CDSS) can generate unprecedented amounts of predictive information about individuals’ health. Yet, despite the potential of these systems to promote proactive decision-making and improve health outcomes, their utility and impact remain poorly understood due to their still rare application in clinical practice. Taking the example of AI-powered CDSS in stroke medicine as a case in point, this paper provides a nuanced account of stroke survivors’, family members’, and healthcare professionals’ expectations and attitudes towards medical AI.

Methods

We followed a qualitative research design informed by the sociology of expectations, which recognizes the generative role of individuals’ expectations in shaping scientific and technological change. Semi-structured interviews were conducted with stroke survivors, family members, and healthcare professionals specialized in stroke based in Germany and Switzerland. Data was analyzed using a combination of inductive and deductive thematic analysis.

Results

Based on the participants’ deliberations, we identified four presumed roles that medical AI could play in stroke medicine, including an administrative, assistive, advisory, and autonomous role AI. While most participants held positive attitudes towards medical AI and its potential to increase accuracy, speed, and efficiency in medical decision making, they also cautioned that it is not a stand-alone solution and may even lead to new problems. Participants particularly emphasized the importance of relational aspects and raised questions regarding the impact of AI on roles and responsibilities and patients’ rights to information and decision-making. These findings shed light on the potential impact of medical AI on professional identities, role perceptions, and the doctor-patient relationship.

Conclusion

Our findings highlight the need for a more differentiated approach to identifying and tackling pertinent ethical and legal issues in the context of medical AI. We advocate for stakeholder and public involvement in the development of AI and AI governance to ensure that medical AI offers solutions to the most pressing challenges patients and clinicians face in clinical care.
First evidence of a monodominant (Englerodendron, Amherstieae, Detarioideae, Leguminosae) tropical moist forest from the early Miocene (21.73 Ma) of Ethiopia
Authors: Pan Aaron D. , Jacobs Bonnie F. , Bush Rosemary T. , de la Estrella Manuel , Grímsson Friðgeir , Herendeen Patrick S. , van der Burgt Xander M. , Currano Ellen D.
Published: January 2023

Many tropical wet forests are species-rich and have relatively even species frequency distributions. But, dominance by a single canopy species can also occur in tropical wet climates and can remain stable for centuries. These are uncommon globally, with the African wet tropics supporting more such communities than the Neotropics or Southeast Asia. Differences in regional evolutionary histories are implied by biogeography: most of Africa’s monodominance-forming species are Amherstieae-tribe legumes; monodominance in Neotropical forests occur among diverse taxonomic groups, often legumes, but rarely Amherstieae, and monodominance in Southeast Asian forests occurs mostly among Dipterocarpaceae species. African monodominant forests have been characterized ecologically and taxonomically, but their deep-time history is unknown despite their significant presence and bottom-up ecological influence on diversity. Herein we describe fossil leaflets ofEnglerodendron mulugetanumsp. nov., an extinct species of the extant genusEnglerodendron(Berlinia Clade, Amherstieae, Detarioideae) from the 21.73 Ma Mush Valley site in Ethiopia. We also document a detailed study of associated legume pollen, which originate from a single taxon sharing characters with more than one extant descendant. Taxonomically, the pollen is most comparable to that from some extantEnglerodendronspecies and supports a likely affiliation with theEnglerodendronmacrofossils. The Mush Valley site provides the first fossil evidence of a monodominant tropical forest in Africa as represented by leaflets and pollen. Previous studies documented >2400 leaves and leaflets from localities at six stratigraphic levels spanning 50,000–60,000 years of nearly continuous deposition within seven meters of section; all but the basal level contain ≥ 50%E.mulugetanumleaflets. Modern leaf litter studies in African mixed vs. monodominant forests indicates the likelihood of monodominance in the forests that surrounded the Mush paleolake, particularly after the basal level. Thus, we provide an early case for monodominance within the Amherstieae legumes in Africa.
Consumers’ acceptance toward whole and processed mealworms: A cross-country study in Belgium, China, Italy, Mexico, and the US
Authors: Tzompa-Sosa Daylan Amelia , Moruzzo Roberta , Mancini Simone , Schouteten Joachim Jietse , Liu Aijun , Li Jie , Sogari Giovanni
Published: January 2023

The interest in edible insects as food is growing, both in traditional and non-traditional insect-eating countries given their advantages in terms of sustainability and nutritional content. However, only a few studies have conducted cross-country investigations on the acceptance of including processed or whole insects in the diet. Thus, this study aimed to examine to which extent consumers were accepting (i) whole and visible mealworms, (ii) processed mealworms in their diet and (iii) to explore the factors affecting the acceptance level of consuming mealworms in countries with and without entomophagy tradition. An online survey was applied to collect responses (3,006) from five countries–i.e., Belgium, China, Italy, Mexico, and the US–using a quota sampling method. Moreover, an information treatment was included with about half of the participants receiving information about the advantages of edible insects as food (ingredient) and the presence of food safety regulations. Across countries, gender was the main factor affecting acceptance level as men accepted mealworms more than women. Entomophagy tradition mainly explained the differences among countries. Countries with entomophagy traditions (Mexico and China) showed higher acceptance of including whole or processed mealworms in the diet compared to countries with no entomophagy traditions (i.e., Belgium, Italy, and the US). While information and age did affect differently the acceptance of including processed mealworms in countries with entomophagy traditions showing that consumer acceptance was affected by information in Mexico and by age in China. Whereas it was found that younger people (below 42 years old) in countries without entomophagy tradition were more open to accepting processed mealworms in their diet. Moreover, across countries, the acceptance of including processed mealworms was higher compared to whole mealworms. These findings provide insights into which consumer segments to target and the potential impact of information when introducing new insect-based foods in countries with and without entomophagy traditions.
The nocturnal life of the great scallops (Pecten maximus, L.): First description of their natural daily valve opening cycle
Authors: Retailleau Elie , Chauvaud Arthur , Richard Gaetan , Mathias Delphine , Chauvaud Laurent , Reynaud Sarah , Mars Jerome , Chauvaud Sylvain
Published: January 2023

Valvometry techniques used to monitor bivalve gaping activity have elucidated numerous relationships with environmental fluctuations, along with biological rhythms ranging from sub-daily to seasonal. Thus, a precise understanding of the natural activity of bivalves (i.e., not exposed to stressful environmental variations) is necessary as a baseline for detecting abnormal behaviors (deviations). This knowledge is also needed to reliably interpret observations of bivalve gaping behavior and associated biological processes (e.g., respiration, nutrition) acquired over time-limited periods. With this in mind, we investigated the natural daily gaping activity of the great scallop (Pecten maximus) by continuously monitoring 35 individuals in several individual tanks and in situ (Bay of Saint-Brieuc, Brittany, France) using fully autonomous Hall effect sensors. Our results revealed a circadian cycle (τ = 24.0h) in scallop gaping activity. Despite significant inter-individual variability in mean opening and cycle amplitude, almost all individuals (87.5%) exhibited nocturnal activity, with valves more open at night than during the day. A shift in light regime in the tanks triggered an instantaneous change in opening pattern, indicating that light levels strongly determine scallop activity. Based on the opening status of scallops, we also identified several gaping behaviors deviating from the regular daily pattern (lack of rhythmicity, high daytime opening), potentially reflecting physiological weakness. While further long-term studies are required to fully understand the natural activity of scallops, these findings pave the way for studies focused on the scallop response to external factors and introduce further research into the detection of abnormal behaviors. Coupling observations of diel valve gaping cycles with other daily variations in organismal and environmental parameters could help explain mechanisms driving the growth patterns of scallops observed in their shell striations. From a technical perspective, our field-based monitoring demonstrates the suitability of autonomous valvometry sensors for studying mobile subtidal bivalve activity in remote offshore environments.
Epidemiology of foodborne bongkrekic acid poisoning outbreaks in China, 2010 to 2020
Authors: Zhang Hexiang , Guo Yunchang , Chen Lili , Liu Zhitao , Liang Junhua , Shi Mengmeng , Gao Fei , Song Yunqi , Chen Jiang , Fu Ping
Published: January 2023

Foodborne bongkrekic acid (BA) poisoning is a fatal foodborne disease in China. From 2010–2020, a total of 19 BA poisoning outbreaks were reported to the China National Foodborne Disease Outbreak Surveillance System. These outbreaks involved 146 illnesses, 139 hospitalizations, and 43 deaths, with a case-fatality rate of 29.5%. Approximately 73.3% of the outbreaks occurred in South and Southwest China. Homemade fermented corn flour products, tremella, and sweet potato flour and corn flour products (jelly) caused more early outbreaks, and novel vehicles (wet rice noodles and Auricularia auricula) were associated with later outbreaks in the study period. Outbreaks most frequently occurred at home (79.0%) and in restaurants (21.0%). The prohibition of traditional processed homemade fermented corn flour products and improvement in bongkrekic acid poisoning case identification and early treatment have resulted in a reduction in the case-fatality rate.
How we compare: A new approach to assess aspects of the comparison process for appearance-based standards and their associations with individual differences in wellbeing and personality measures
Authors: McCarthy Peter A. , Meyer Thomas , Back Mitja D. , Morina Nexhmedin
Published: January 2023

We introduce a novel approach to assess habitual comparison processes, while distinguishing between different types of comparison standards. Several comparison theories (e.g., social) suggest that self-evaluations use different standards to inform self-perception and are associated with wellbeing and personality. We developed the Comparison Standards Scale for Appearance (CSS-A) to examine self-reported engagement with social, temporal, criteria-based, dimensional, and counterfactual comparisons for upward and downward standards in relation to appearance. The scale was completed by three hundred participants online alongside measures of appearance schemas, social comparison evaluations, depression, anxiety, stress, self-esteem, physical self-concept, narcissism, and perfectionism. The CSS-A was found to reliably assess individual differences in upward and downward comparison frequency and affective impact for multiple comparison standards. In line with theory, CSS-A upward comparisons were more frequent than downward comparisons and coincided with negative (versus positive) affective impact. Comparison intensity (i.e., comparison frequency × discrepancy) predicted negative and positive affective impact for upward and downward comparisons, respectively. This relationship was partially mediated by appearance concern for upward comparisons (a composite of appearance schemas and physical self-concept), yet moderated by negativity for downward comparisons (a composite of depression, anxiety, stress, and self-esteem). We offer a framework for measuring the comparison process that warrants further research on underlying comparison processes, for which the CSS(-A) and experience sampling methods should serve as useful tools.
Pre-/perinatal reduced optimality and neurodevelopment at 1 month and 3 years of age: Results from the Japan Environment and Children’s Study (JECS)
Authors: Yasumitsu-Lovell Kahoko , Thompson Lucy , Fernell Elisabeth , Eitoku Masamitsu , Suganuma Narufurmi , Gillberg Christopher ,   on behalf of the Japan Environment and Children’s Study Group
Published: January 2023

Neurodevelopmental disorders (NDDs) in children are associated with a complex combination of genetic and/or environmental factors. Pre-/perinatal events are major known environmental suboptimal factors, and their individual and combined contributions vary. This study investigated the association between pre-/perinatal reduced optimality and child development observed by parents at 1 month, as well as NDDs at 3 years of age (i.e., motor delay, intellectual disability, developmental language disorder, and autism spectrum disorder), in the context of the Japan Environment and Children’s Study. The study also assessed whether child development at 1 month predicted NDDs at 3 years of age. Associations between 25 pre-/perinatal factors and (a) developmental concerns at 1 month of age and (b) NDDs at 3 years were analyzed (n = 71,682). Binomial regression models were used to investigate risk ratios of the developmental outcome at each time point for total pre-/perinatal reduced optimality scale scores, as well as for individual pre-/perinatal factors of the reduced optimality scale. Finally, we assessed the ability of parental observations of offspring development at 1 month to predict NDDs at 3 years. Total reduced optimality scores were positively associated with 1-month developmental concerns and 3-year NDDs, with higher scores (i.e., a reduction in optimality) associated with an increased risk of both NDDs and earlier parental concerns. Neonatal transportation, epidural analgesia, advanced maternal age, cesarean section delivery, Apgar score ≤8, and hyperbilirubinemia were identified as individual risk factors for 3-year NDDs, overlapping with 14 risk factors for 1-month developmental concerns except Apgar score ≤8. Among six developmental items assessed at 1 month of age, concerns about gross motor function and difficulty holding/trouble calming down had the strongest associations with later-diagnosed motor delay and autism spectrum disorder, respectively. Five perinatal factors and advanced maternal age were associated with NDD at 3 years of age, as were early parental developmental concerns regarding their offspring’s overall development, indicating the importance of careful follow-up of offspring born with pre-/perinatal reduced optimality. The results also implicated early parental concerns, as early as 1 month, may also be a useful indicator of later NDD status.
Social burden of three major diseases in Japan: A time trend and future projections using the comprehensive cost of illness method
Authors: Hirata Koki , Matsumoto Kunichika , Hatakeyama Yosuke , Onishi Ryo , Seto Kanako , Hasegawa Tomonori
Published: January 2023

Background

Three major diseases in Japan, cancer, heart disease, and cerebrovascular disease (CVD) are the leading causes of death in Japan. This study aimed to clarify the social burden of these diseases, including long-term care (LTC), and to predict future trends.

Methods

The comprehensive cost of illness (C-COI), a modification of the cost of illness (COI), was used to estimate the social burden of the three major diseases in Japan. The C-COI can macroscopically estimate both direct and indirect costs, including the LTC. A new method for future projections of the C-COI was developed according to the method for future projections of the COI. All data sources were government statistics.

Results

The C-COI of cancer, heart diseases, and CVD in 2017 amounted to 11.0 trillion JPY, 5.3 trillion JPY, and 6.5 trillion JPY, respectively. The projected future C-COI in 2029 was 10.3 trillion JPY, 5.3 trillion JPY, and 4.4 trillion JPY, respectively. In 2029, the LTC costs accounted for 4.4%, 12.8%, and 44.1% of the total C-COI, respectively. Informal care costs are projected to be approximately 1.7 times higher, assuming that all family caregivers will be replaced by professional caregivers in 2029.

Conclusion

Indirect costs for all three diseases were projected to decrease owing to aging of the patient. In contrast to the other two diseases, the LTC cost of CVD accounted for a large proportion of the burden. The burden of CVD is expected to decrease in the future, but informal care by older family caregivers is suggested to reach its limits. In the future, the focus of resource allocation should shift from medical care to LTC, especially support for family caregivers. A method of future projections for the social burden based on the C-COI was considered effective for identifying issues for healthcare policy in the context of the times.
The role of the social environment in inclusive sports participation—Identifying similarities and challenges in athletes with and without Intellectual Disabilities through coaches’ eyes: A qualitative inquiry
Authors: Sakalidis Kandianos Emmanouil , Fadeeva Anastasia , Hettinga Florentina Johanna , Ling Fiona Chun Man
Published: January 2023

Despite the efforts of mainstreaming in sports, inclusive participation of people with Intellectual Disabilities (ID) in sports remains challenging. In sports settings, the social environment may influence the motivation of athletes and promote (or restrict) inclusive sports participation of athletes with ID. Thus, this study aims to explore the motivations of athletes and coaches and to investigate the role of the social environment in sports participation of athletes with and without ID. Coaches who coach athletes with ID (n = 11), athletes without ID (n = 13) and both groups (n = 2) were involved in semi-structured interviews. From the inductive thematic analysis three themes were identified for the sports motivations of athletes with ID (Sport-related Progression, Social Interaction, Positive Emotions), two for their coaches’ motivations (Help others, Personal and Professional Development) and four for coaching practices toward athletes with ID (Psychological and Life skills development, Building Meaningful Relationships, Behaviour adaptations and Participation-focused). Moreover, ‘Teammates and Opponents’ and ‘Family’ were classified as social agents that influence the sport participation of athletes with ID. Coaches of athletes without ID reported additional themes about their athletes’ motivations (Health-related Reasons), their coaching motivations (Career aspirations) and their coaching practices towards athletes without ID (Performance-focused). The motivations of athletes and the practices of coaches could promote sports participation of athletes with ID, but more work is needed to address athletes’ sports motivations and overcome the able-ist attitudes and the social oppression that may make their inclusion in sports more challenging. These sports participation barriers and facilitators could provide direction to stakeholders for developing inclusive sports pathways to people with ID.
G-quadruplexes formed by Varicella-Zoster virus reiteration sequences suppress expression of glycoprotein C and regulate viral cell-to-cell spread
Authors: Chung Woo-Chang , Ravichandran Subramaniyam , Park Daegyu , Lee Gwang Myeong , Kim Young-Eui , Choi Youngju , Song Moon Jung , Kim Kyeong Kyu , Ahn Jin-Hyun
Published: January 2023

G-quadruplex (G4) formed by repetitive guanosine-rich sequences plays important roles in diverse cellular processes; however, its roles in viral infection are not fully understood. In this study, we investigated the genome-wide distribution of G4-forming sequences (G4 motifs) in Varicella-Zoster virus (VZV) and found that G4 motifs are enriched in the internal repeat short and the terminal repeat short regions flanking the unique short region and also in some reiteration (R) sequence regions. A high density of G4 motifs in the R2 region was found on the template strand of ORF14, which encodes glycoprotein C (gC), a virulent factor for viral growth in skin. Analyses such as circular dichroism spectroscopy, thermal difference spectra, and native polyacrylamide gel electrophoresis with oligodeoxynucleotides demonstrated that several G4 motifs in ORF14 form stable G4 structures. In transfection assays, gC expression from the G4-disrupted ORF14 gene was increased at the transcriptional level and became more resistant to suppression by G4-ligand treatment. The recombinant virus containing the G4-disrupted ORF14 gene expressed a higher level of gC mRNA, while it showed a slightly reduced growth. This G4-disrupted ORF14 virus produced smaller plaques than the wild-type virus. Our results demonstrate that G4 formation via reiteration sequences suppresses gC expression during VZV infection and regulates viral cell-to-cell spread.
Subcultural event tourism: The case study of Monte Paradiso hardcore punk festival in Pula, Croatia
Authors: Vojnović Nikola
Published: January 2023

This article analyses the Monte Paradiso hardcore punk festival in the city of Pula as an important subcultural event for the punk scene and its impact on tourism. The research aims to determine the importance of the festival in relation to tourism supply and the specific spatial features of this event. Pula was a military and industrial city, but it later became famous as a festival destination. In the summer of 1992, a group of punk enthusiasts organized the first Monte Paradiso festival. The event took place in an abandoned Austro-Hungarian fortress. That venue hosted the festival every summer from 1992 to 2000. The festival programme attracted numerous punk fans, but its impact on tourism was irrelevant. Since 2001, the festival has been organized in the Karlo Rojc Community Centre, housed in a former Yugoslav army building in the Monte Zaro urban district. The findings indicate that the festival has become a part of the city’s tourist promotion and the rebranding of Pula.
Rapid Immunochromatography on Fresh Intestinal Biopsy Is Highly Accurate in Detecting Intestinal Antitransglutaminase Antibodies
Authors: Lega Sara , De Leo Luigina , Ziberna Fabiana , Grigoletto Veronica , Gaita Bartolomea , Giangreco Manuela , Villanacci Vincenzo , Bramuzzo Matteo , Di Leo Grazia , Not Tarcisio
Published: January 2023

INTRODUCTION:

Intestinal antitransglutaminase antibodies (I-anti-TG2) are a specific marker of celiac disease (CeD). The aim of this study was to evaluate the diagnostic accuracy of a novel application of an immunochromatographic assay referred to as Rapid_AntiTG2 to detect I-anti-TG2 on intestinal biopsy lysate.

METHODS:

Consecutive pediatric patients referred to a single center for elective upper endoscopy were enrolled. Biopsies were taken from duodenal bulb and distal duodenum. For each sampling site, 2 biopsies were analyzed for standard histology, 1 biopsy was cultured to perform the reference standard assay for I-anti-TG2 detection (endomysium [EMA] biopsy), and 1 biopsy was mechanically lysed to perform Rapid_AntiTG2. The primary outcome was the diagnostic accuracy of Rapid_AntiTG2 on biopsy lysate compared with that of the gold standard (serology + histopathology) for CeD diagnosis. The secondary outcome was the agreement of Rapid_AntiTG2 with EMA biopsy.

RESULTS:

One hundred forty-eight patients were included. Of them, 79 were those with CeD (64 classical CeD, 2 seronegative CeD, and 13 potential CeD) and 69 were controls. Rapid_AntiTG2 on biopsy lysate had very high diagnostic accuracy (sensitivity 100%, specificity 97%, LR+ 34.1, LR− 0.01) in separating patients with CeD from controls. Diagnostic accuracy was unchanged in patients with potential and seronegative CeD. Rapid_AntiTG2 on biopsy lysate had almost perfect agreement with the EMA biopsy reference test (99% agreement, Cohen K 0.97).

DISCUSSION:

I-anti-TG2 can be detected with an immunochromatographic assay after simple mechanical lysis of fresh intestinal biopsy with very high diagnostic accuracy. The test is quick and easy to perform and can be widely available in any endoscopy unit. Its implementation would allow a better understanding of the prognostic value of I-anti-TG2 and help clinicians in cases of suspected CeD that are difficult to classify.