bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vivo Characterization of a Red Light-Activated Vasodilation: A Photobiomodulation Study
Authors: Keszler Agnes , Lindemer Brian , Broeckel Grant , Weihrauch Dorothee , Gao Yan , Lohr Nicole L.
Published: May 2022

Nitric oxide dependent vasodilation is an effective mechanism for restoring blood flow to ischemic tissues. Previously, we established an ex vivo murine model whereby red light (670 nm) facilitates vasodilation via an endothelium derived vasoactive species which contains a functional group that can be reduced to nitric oxide. In the present study we investigated this vasodilator in vivo by measuring blood flow with Laser Doppler Perfusion imaging in mice. The vasodilatory nitric oxide precursor was analyzed in plasma and muscle with triiodide-dependent chemiluminescence. First, a 5–10 min irradiation of a 3 cm2 area in the hind limb at 670 nm (50 mW/cm2) produced optimal vasodilation. The nitric oxide precursor in the irradiated quadriceps tissue decreased significantly from 123 ± 18 pmol/g tissue by both intensity and duration of light treatment to an average of 90 ± 17 pmol/g tissue, while stayed steady (137 ± 21 pmol/g tissue) in unexposed control hindlimb. Second, the blood flow remained elevated 30 min after termination of the light exposure. The nitric oxide precursor content significantly increased by 50% by irradiation then depleted in plasma, while remained stable in the hindlimb muscle. Third, to mimic human peripheral artery disease, an ameroid constrictor was inserted on the proximal femoral artery of mice and caused a significant reduction of flow. Repeated light treatment for 14 days achieved steady and significant increase of perfusion in the constricted limb. Our results strongly support 670 nm light can regulate dilation of conduit vessel by releasing a vasoactive nitric oxide precursor species and may offer a simple home-based therapy in the future to individuals with impaired blood flow in the leg.
Modulation of Wheat Yield Components in Response to Management Intensification to Reduce Yield Gaps
Authors: Jaenisch Brent R. , Munaro Lucas B. , Jagadish S. V. Krishna , Lollato Romulo P.
Published: May 2022

Appropriate genotype selection and management can impact wheat (Triticum aestivum L.) yield in dryland environments, but their impact on yield components and their role in yield modulation are not well understood. Our objectives were to evaluate the yield response of commercial winter wheat genotypes to different management practices reflecting a stepwise increase in management intensity (including a reduction in crop density under high input), and to quantify how the different yield components modulate wheat yield. A factorial experiment evaluated six management (M) intensities [“farmer practice” (FP), “enhanced fertility” (EF), “ecological intensification” (EI), “increased foliar protection” (IFP), “water-limited yield” (Yw), and “increased plant productivity” (IPP)] and four winter wheat genotypes (G) in four Kansas environments (E). Average grain yield was 4.9 Mg ha–1 and ranged from 2.0 to 7.4 Mg ha–1, with significant two-way interactions (E × M and E × G). The EF usually maximized yields in dry environments, while EI, which consisted of EF plus one fungicide application, maximized yields in environments with greater water availability. Across all sources of variation, kernels m–2 and aboveground biomass were the strongest modulators of yield as compared to kernel weight and harvest index, while spikes m–2 and kernels spike–1 modulated yields at a similar magnitude. Foliar fungicides improved yield through increased green canopy cover duration and greater radiation intercepted during grain filling. When crop density was reduced from 2.7 to 1.1 million plants per hectare in an otherwise high-input system, plants produced more productive tillers (with genotype-specific response); however, reduced green canopy cover at anthesis and reduced cumulative solar radiation intercepted during grain filling limited wheat yield—although large differences in canopy cover or intercepted radiation were needed to cause modest changes in yield. Treatments more intensive than EI were not warranted as EF or EI maximized yields at all environments, and practices that promote biomass and kernels m–2 are to be targeted for future increases in wheat yield.
Deep Learning and Hyperspectral Images Based Tomato Soluble Solids Content and Firmness Estimation
Authors: Xiang Yun , Chen Qijun , Su Zhongjing , Zhang Lu , Chen Zuohui , Zhou Guozhi , Yao Zhuping , Xuan Qi , Cheng Yuan
Published: May 2022

Cherry tomato (Solanum lycopersicum) is popular with consumers over the world due to its special flavor. Soluble solids content (SSC) and firmness are two key metrics for evaluating the product qualities. In this work, we develop non-destructive testing techniques for SSC and fruit firmness based on hyperspectral images and the corresponding deep learning regression model. Hyperspectral reflectance images of over 200 tomato fruits are derived with the spectrum ranging from 400 to 1,000 nm. The acquired hyperspectral images are corrected and the spectral information are extracted. A novel one-dimensional (1D) convolutional ResNet (Con1dResNet) based regression model is proposed and compared with the state of art techniques. Experimental results show that, with a relatively large number of samples our technique is 26.4% better than state of art technique for SSC and 33.7% for firmness. The results of this study indicate the application potential of hyperspectral imaging technique in the SSC and firmness detection, which provides a new option for non-destructive testing of cherry tomato fruit quality in the future.
Novel Trichoderma Isolates Alleviate Water Deficit Stress in Susceptible Tomato Genotypes
Authors: Rawal Ranjana , Scheerens Joseph C. , Fenstemaker Sean M. , Francis David M. , Miller Sally A. , Benitez Maria-Soledad
Published: May 2022

Symbiotic fungi in the genus Trichoderma can induce abiotic stress tolerance in crops. The beneficial effects of Trichoderma on water deficit stress are poorly understood and may be isolate-specific. Our objective was to evaluate a collection of Nepalese Trichoderma isolates and their efficacy to improve tomato (Solanum lycopersicum) growth under water deficit. Variable growth in low moisture environments was observed among Trichoderma isolates from Nepal, Ohio, and commercial sources using in vitro assays. The overall performance of the population decreased when cultured under conditions of decreasing matric water potential (0.0, –2.8, –4.8, and –8.5 Ψ). Twelve isolates were selected for evaluation for their potential to elicit drought tolerance in greenhouse-grown ‘Roma Organic’ tomatoes. Plants treated with T. asperelloides-NT33 had higher shoot weight than the non-inoculated control (T0) under water deficit stress conditions. Further, the stress-reducing efficacy of isolates T. asperelloides-NT33, T. asperellum-NT16, T. asperelloides-NT3, and commercial T. harzianum-T22 were tested on tomato genotypes with differing tolerance to drought [‘Roma Organic,’ ‘Jaune Flamme,’ and ‘Punta Banda’]. The water deficit susceptible genotypes ‘Roma Organic’ and ‘Jaune Flamme’ inoculated with isolate NT33 had significantly higher shoot weight (37 and 30% respectively; p < 0.05) compared to the non-inoculated control under water deficit stress conditions. In drought tolerant ‘Punta Banda,’ shoot weight was also significantly greater in NT33 inoculated plants under water deficit stress conditions, but with lower magnitude difference (8%; p < 0.05). Our results demonstrate differences in the ability of Trichoderma isolates to confer tolerance to water deficit in tomato with NT33 potentially relieving stress. Tomato genotypes also play a role in the outcome of interactions with the Trichoderma isolates we tested.
Two Liberibacter Proteins Combine to Suppress Critical Innate Immune Defenses in Citrus
Authors: Basu Supratim , Huynh Loan , Zhang Shujian , Rabara Roel , Nguyen Hau , Velásquez Guzmán Jeanette , Hao Guixia , Miles Godfrey , Shi Qingchun , Stover Ed , Gupta Goutam
Published: May 2022

We adopted a systems-based approach to determine the role of two Candidatus Liberibacter asiaticus (CLas) proteins, LasP235 and Effector 3, in Huanglongbing (HLB) pathogenesis. While a published work suggests the involvement of these CLas proteins HLB pathogenesis, the exact structure-based mechanism of their action has not been elucidated. We conducted the following experiments to determine the structure-based mechanisms of action. First, we immunoprecipitated the interacting citrus protein partners of LasP235 and Effector 3 from the healthy and CLas-infected Hamlin extracts and identified them by Liquid Chromatography with tandem mass spectrometry (LC–MS/MS). Second, we performed a split green fluorescent protein (GFP) assay in tobacco to validate that the interactions observed in vitro are also retained in planta. The notable in planta citrus targets of LasP235 and Effector 3 include citrus innate immune proteins. Third, in vitro and in planta studies were performed to show that LasP235 and Effector 3 interact with and inhibit the functions of multiple citrus proteins belonging to the innate immune pathways. These inhibitory interactions led to a high level of reactive oxygen species, blocking of bactericidal lipid transfer protein (LTP), and induction of premature programed cell death (PCD), all of which are beneficial to CLas lifecycle and HLB pathogenesis. Finally, we performed molecular dynamics simulations to visualize the interactions of LasP235 and Effector 3, respectively, with LTP and Kunitz protease inhibitor. This led to the design of an LTP mimic, which sequestered and blocked LasP235and rescued the bactericidal activity of LTP thereby proving that LasP235, indeed, participates in HLB pathogenesis.
The Future Looks Good: Examining the Associations Between Coping, Psychological Distress, and Optimism
Authors: Santos Tiago , Uva António de Sousa , Rodrigues José Fernandes , Ferreira Regina , Monteiro Diogo , Hernández-Mendo Antonio , Rodrigues Filipe
Published: May 2022

The recent pandemic and consequent lockdown had a substantial impact on mental health and optimism regarding the future. Previous research showed that levels of depression, anxiety, and stress had increased throughout the pandemic. Nonetheless, how individuals cope when faced with adversity may be associated with positive expectations regarding the future. A sample of 274 Portuguese workers (female = 54) with a mean age of 40.86 (SD = 0.70) and work experience of 19.68 years (SD = 12.07) met inclusion criteria and agreed to participate in the proposed study. They represented a variety of working classes (i.e., arts, engineering, marketers, transportation and logistic, clerks, personal trainers, managers, lawyers, accountants, directors, journalism, health care). We investigated the associations between depression, stress, anxiety, adaptive and maladaptive coping, and optimism while controlling for working experience, gender, and work type. We found that depression was related to lower levels of optimism. However, for participants scoring high on adaptive coping and anxiety, higher scores of optimism were reported. Gender, work type, and experience did not significantly influence the results. These results provide evidence through which positive mental health can be promoted after the pandemic.
The Contribution of Psychological Wellbeing and Emotion-Regulation to Foreign Language Teaching Enjoyment
Authors: Azari Noughabi Mostafa , Fekri Neda , Kazemkhah Hasankiadeh Fatemeh
Published: May 2022

The notion of foreign language teaching enjoyment (FLTE) has recently been introduced to the field of foreign language education as a positive emotion which influences teachers’ professional and psychological growth. Due to the pure novelty of the concept, scant research attention has been paid to its antecedents in English as a foreign language (EFL) contexts. The present study aims to investigate the extent to which psychological wellbeing and emotion regulation can contribute to FLTE of Iranian EFL teachers. The participants (N = 575) responded to three electronic questionnaires and the collected data were then analyzed through structural equation modeling (SEM). The SEM analysis indicated that Iranian EFL teachers’ psychological wellbeing and emotion regulation significantly influenced their FLTE. The findings revealed that psychological wellbeing was a stronger predictor of EFL teachers’ FLTE in the Iranian context. The findings were discussed regarding the causes of increasing teaching enjoyment. The results highlighted the importance of applying psychology in language teaching research. Finally, implications and suggestions for future research are offered.
Case Report: A Case of Valproic Acid-Induced Hyperammonemic Encephalopathy Associated With the Initiation of Lithium: A Re-duplicable Finding
Authors: Levy Anna , Very Etienne , Montastruc François , Birmes Philippe , Jullien Adeline , Richaud Louis
Published: May 2022

Introduction

Hyperammonemic encephalopathy (HAE) is a serious adverse effect of valproate semisodium, which is facilitated by the potential for drug interaction. However, despite frequent co-prescription of valproate semisodium and lithium, the role of this combination in the occurrence of HAE has not been defined in the literature. This case report concerns the occurrence of HAE concomitant with the initiation of lithium in a 29-year-old patient who had been placed on valproate semisodium for a schizoaffective disorder.

Case Report

Due to a relapse while on a combined antipsychotic and mood-stabilizing therapy (paliperidone palmitate and valproate semisodium), a cross-taper from valproate semisodium to lithium was proposed. The initiation of lithium was accompanied by an acute confusional syndrome, an elevated serum valproate level and hyperammonemia suggestive of drug-induced HAE. The discontinuation of lithium and reduction of valproate semisodium led to neurological improvement, until a recrudescence of psychiatric symptoms justified a rechallenge of the combination within the framework of a new cross-taper. As soon as Lithium was re-initiated, an increase in the serum valproate level and hyperammonemia were again noted.

Discussion

The mechanisms of valproate-related HAE involve various metabolic pathways. In this case, exploration of the iatrogenic hypothesis focused on the imputability of concomitant cannabis use and co-prescriptions of benzodiazepines, antipsychotics, and in all likelihood, mood stabilizers.

Conclusion

Therefore, this case study suggests that Lithium plays a role in serum valproate level elevation, and supports the hypothesis of an association between an elevated serum valproate level, hyperammonemia and reversible encephalopathy. A more in-depth pharmacokinetic exploration would provide a better understanding of the mechanisms of these interactions and support for the benefit-risk balance associated with this frequent co-prescription.
Online One-Stop Shop for Disaster Response Services After the MH17 Airplane Crash: An Evaluation Study
Authors: van Herpen Merel M. , Dückers Michel L. A. , Schaap Rick , Olff Miranda , te Brake Hans
Published: May 2022

Background

A one-stop shop for disaster response services provides a central location for information and advice in an accessible way. Yet little is known about its organization and outcomes. After the MH17 airplane crash, the one-stop shop concept was realized through a digital environment called the Information and Referral Center (IRC). The aim of this study was to evaluate the experiences of users and providers in regard to the IRC and to identify improvement points for future IRCs.

Method

Data was collected among affected ones as well as involved organizations, using interviews, focus groups, surveys and online user information. Existing evaluation and quality models were combined to design the study and analyze the data.

Results

First, affected ones and a variety of organizations involved were positive about the merits of the IRC. Affected ones indicated they perceived the IRC as a reliable source of information and appreciated the referral possibilities. Second, the feature of the IRC to serve as a community where affected ones could meet, share experiences and support each other was hardly used according to participants. Lastly, tracking evolving psychosocial needs and problems through the IRC was hampered due to difficulty in accessing relevant data.

Conclusions

The IRC helped organizations to structure and align their services. Affected ones were positive about its reliability and accessibility. An IRC has to be embedded within the established care structures. Future research could indicate whether an IRC is useful in other event types and population contexts as well.
The Relationships Between Family Characteristics and Undergraduate Students' COVID-19 Responses: A Cross-Sectional Study in China
Authors: Zhao Teng , Su Qiang , Hu Xinye
Published: May 2022

The COVID-19 pandemic has dramatically threatened the post-secondary education setting. It is crucial to understand the factors that potentially affect college students' COVID-19 responses, such as risk awareness, knowledge of the disease, and pandemic preparedness. However, there is insufficient literature on whether family characteristics contribute to students' COVID-19 responses. Leveraging the data from self-administrated survey – titled College Students' Epidemic Preparedness in the Post-COVID-19 Era (CSEPPCE), we find that students from high-income families were more likely to have a greater awareness of risk and better knowledge of COVID-19. Additionally, students whose parents were employed by the government had a higher probability of knowing COVID-19 symptoms and wearing masks. However, the relationships among risk awareness, knowledge, and family income did not meaningfully vary by sex or ethnicity. Implications and future directions are discussed.
Development and Internal Validation of Supervised Machine Learning Algorithm for Predicting the Risk of Recollapse Following Minimally Invasive Kyphoplasty in Osteoporotic Vertebral Compression Fractures
Authors: Dong Sheng-tao , Zhu Jieyang , Yang Hua , Huang Guangyi , Zhao Chenning , Yuan Bo
Published: May 2022

Background

The published literatures indicate that patients with osteoporotic vertebral compression fractures (OVCFs) benefit significantly from percutaneous kyphoplasty (PKP), but this surgical technique is associated with frequent postoperative recollapse, a complication that severely limits long-term postoperative functional recovery.

Methods

This study retrospectively analyzed single-segment OVCF patients who underwent bilateral PKP at our academic center from January 1, 2017 to September 30, 2019. Comparing the plain films of patients within 3 days after surgery and at the final follow-up, we classified patients with more than 10% loss of sagittal anterior height as the recollapse group. Univariate and multivariate logistic regression analyses were performed to determine the risk factors affecting recollapse after PKP. Based on the logistic regression results, we constructed one support vector machine (SVM) classifier to predict recollapse using machine learning (ML) algorithm. The predictive performance of this prediction model was validated by the receiver operating characteristic (ROC) curve, 10-fold cross validation, and confusion matrix.

Results

Among the 346 consecutive patients (346 vertebral bodies in total), postoperative recollapse was observed in 40 patients (11.56%). The results of the multivariate logistical regression analysis showed that high body mass index (BMI) (Odds ratio [OR]: 2.08, 95% confidence interval [CI]: 1.58–2.72, p < 0.001), low bone mineral density (BMD) T-scores (OR: 4.27, 95% CI: 1.55–11.75, p = 0.005), presence of intravertebral vacuum cleft (IVC) (OR: 3.10, 95% CI: 1.21–7.99, p = 0.019), separated cement masses (OR: 3.10, 95% CI: 1.21–7.99, p = 0.019), cranial endplate or anterior cortical wall violation (OR: 0.17, 95% CI: 0.04–0.79, p = 0.024), cement-contacted upper endplate alone (OR: 4.39, 95% CI: 1.20–16.08, p = 0.025), and thoracolumbar fracture (OR: 6.17, 95% CI: 1.04–36.71, p = 0.045) were identified as independent risk factors for recollapse after a kyphoplasty surgery. Furthermore, the evaluation indices demonstrated a superior predictive performance of the constructed SVM model, including mean area under receiver operating characteristic curve (AUC) of 0.81, maximum AUC of 0.85, accuracy of 0.81, precision of 0.89, and sensitivity of 0.98.

Conclusions

For patients with OVCFs, the risk factors leading to postoperative recollapse were multidimensional. The predictive model we constructed provided insights into treatment strategies targeting secondary recollapse prevention.
Pediatric Chronic Pain, Resilience and Psychiatric Comorbidity in Canada: A Retrospective, Comparative Analysis
Authors: Young Megan A. , Anang Polina , Gavalova Anna
Published: May 2022

Objective

Chronic pain compromises child and adolescent well-being and development. This study aimed to identify risk factors for chronic pain and exploration of how young people negotiate such risks and express resilience. We hypothesized children and youth with chronic pain would report greater prevalence of mental health disorders than the general population; and those demonstrating greater resilience would demonstrate less psychiatric comorbidity.

Method

A retrospective chart review was conducted for all patients (ages 7–17) attending the sole pediatric chronic pain clinic in Manitoba, from 2015 to 2019 (N = 116). Patients' demographic information and psychiatric illness burden were compared to provincial epidemiological data using Chi-Square tests. Pain sites, family history, psychiatric illness, psychosocial functioning, treatment history and treatment recommendations were explored.

Results

The sample was predominantly female (74%; N = 114). Sixty-eight percent of patients reported a family history of chronic pain. Thirty-seven percent of the patients (vs. 14.0% anticipated; N = 326 260) reported comorbid psychiatric disorder, X2 (1, N = 114) = 53.00, p < 0.001. Thirty-two percent reported diagnosis of mood and/or anxiety disorder (vs. 7.3%), X2 (1, N = 114) = 99.34, p < 0.001. Children and youth demonstrating resilience through engagement in more prosocial behaviors reported fewer psychiatric symptoms (rs = −0.292, N = 114, p = 0.002, Spearman's correlation).

Conclusions

Female sex, family history, and lower socioeconomic status were associated with chronic pain. Psychiatric conditions were more prevalent in chronic pain patients than in the general population. Approaching chronic pain from a mind-body perspective, while building on patients' strengths, is central to informing treatment.
What Makes Cities Sustainable? Empirical Evidence From a Brazilian Context
Authors: Agostinho Feni , Pierucci Pedro , Fonseca Tamara , Almeida Cecilia M. V. B. , Giannetti Biagio F.
Published: May 2022

About 70% of the world's population will live in urban areas by 2050, emphasizing the importance of assessment tools to guide decisions toward more sustainable cities. The “Mandala ODS” is an alternative tool promoted by the Brazilian government to quantify the sustainability of cities based on the UN SDGs. Although diagnoses are important steps for governance, the behavioral profile of decision makers also appears as a key aspect. This work aims to assess the potential association evidence among biophysical, socioeconomic and cultural variables with city sustainability as measured by the “Mandala ODS.” A sample of 130 Brazilian cities is considered as a sample, and the Pearson's chi-square indicator is calculated for association analysis. Results show that Brazilian cities located in the South/Southeast/Midwest regions, with HDI higher than 0.75, incidence of poverty lower than 40%, territorial area lower than 3,000 km2, and GDP/capita higher than 25,000 R$/person.yr, have significant statistical association that leads them to higher levels of sustainability than other cities without these characteristics. Population density, academic background of mayors, their political ideology and gender showed no association with city sustainability. These findings highlight the fundamental role of governance focused on local characteristics instead of standardized and larger scale based public policies that would hardly bring the same benefits for all cities with different socioeconomic and cultural characteristics. Although additional efforts are still needed to achieve a more comprehensive picture, this work contributes to the discussions about the reasons that lead some cities to achieve higher degrees of sustainability than others.
The Protective Effects of Three Polysaccharides From Abrus cantoniensis Against Cyclophosphamide-Induced Immunosuppression and Oxidative Damage
Authors: Qu Dongshuai , Hu Hongjie , Lian Shuaitao , Sun Wenjing , Si Hongbin
Published: May 2022

This study was designed to systematically elucidate the immunomodulatory and antioxidant effects of three polysaccharide fractions (ACP60, ACP80, and ACPt2) from Abrus cantoniensis on cyclophosphamide (CTX)-induced immunosuppressive mice. The experimental mice were divided into 12 groups, then modeled and administrated with different doses of three polysaccharides (50, 150, 300 mg/kg/day) by gavage. The results showed that ACP could markedly recover the CTX-induced decline in immune organ and hemocytes indexes and promote proliferation of splenocytes, earlap swelling rate, secretion of cytokines (TNF-α, IFN-γ, IL-1β, IL-6), and immunoglobulin (Ig-M and Ig-G). Additionally, ACP improved the enzymatic activities of T-SOD and GSH-PX greatly, while the level of MDA was significantly decreased in the liver. In particular, ACPt2 had higher immunomodulatory and antioxidant activities than ACP60 and ACP80. Based on the present findings, ACP could be utilized as an efficacious candidate for immunomodulators and antioxidants, which provide a new application prospect in the food and pharmaceutical industries.
A Machine Learning-Based Approach for Classification of Focal Splenic Lesions Based on Their CT Features
Authors: Burti Silvia , Zotti Alessandro , Bonsembiante Federico , Contiero Barbara , Banzato Tommaso
Published: May 2022

The aim of the study was to describe the CT features of focal splenic lesions (FSLs) in dogs in order to predict lesion histotype. Dogs that underwent a CT scan and had a FSL diagnosis by cytology or histopathology were retrospectively included in the study. For the statistical analysis the cases were divided into four groups, based on the results of cytopatholoy or hystopathology, namely: nodular hyperplasia (NH), other benign lesions (OBLs), sarcoma (SA), round cell tumour (RCT). Several qualitative and quantitative CT features were described for each case. The relationship occurring between each individual CT feature and the histopathological groups was explred by means of c chi-square test for the count data and by means of Kruskal-Wallis or ANOVA for the continuous data. Furthermore, the main features of each group were described using factorial discriminant analysis, and a decision tree for lesion classification was then developed. Sarcomas were characterised by large dimensions, a cystic appearance and an overall low post contrast-enhancement. NH and OBLs were characterised by small dimensions, a solid appearance and a high post-contrast enhancement. OBLs showed higher post-contrast values than NH. Lastly, RCTs did not exhibit any distinctive CT features. The proposed decision tree had a high accuracy for the classification of SA (0.89) and a moderate accuracy for the classification of OBLs and NH (0.79), whereas it was unable to classify RCTs. The results of the factorial analysis and the proposed decision tree could help the clinician in classifying FSLs based on their CT features. A definitive FSL diagnosis can only be obtained by microscopic examination of the spleen.
Spatial Distribution and Habitat Selection of Sarda Cattle in a Silvopastoral Mediterranean Area
Authors: Acciaro Marco , Bragaglio Andrea , Pittarello Marco , Marrosu Gian Marco , Sitzia Maria , Sanna Gabriele , Decandia Mauro , Bagella Simonetta , Lombardi Giampiero
Published: May 2022

The beef livestock system in Sardinia is based on suckler cows, often belonging to autochthonous breeds, such as the Sarda breed, and they often graze silvopastoral areas. Besides beef meat, silvopastoral systems (SPSs) provide several Ecosystem Services (ESs), such as timber provision, harvested as wood, and watershed protection. Livestock distribution is a critical factor for the sustainable use of SPSs (e.g., to avoid uneven grazing patterns) and information on patterns of spatial use are required. A study was conducted to determine: (i) the spatial distribution and (ii) the habitat selection of Sarda cattle grazing in a Mediterranean silvopastoral area. Over different seasons, 12 free-roaming adult Sarda cows were fitted with Global Positioning System (GPS) Knight tracking collars to calculate an index mapping of the incidence of livestock in the landscape (LRI) and a preference index (PI) for different areas. Since the PI data were not normally distributed, the Aligned Rank Transform (ART) procedure was used for the analysis. LRI was able to represent the spatial variability in resource utilization by livestock as a LRI map. Overall, the areas where the animals drank and received supplementation were strongly preferred by the cows, reaching PI values in the summer of 19.3 ± 4.9 (median ± interquartile range), whereas areas with predominantly rocks were strongly avoided (the worst PI value in the spring was 0.2 ± 0.6). Grasslands were, in general, used in proportion to their presence in the area, with slightly increased use in the spring (PI 1.1 ± 0.5). Forest area was avoided by cows, except in the spring when it was used in proportion to their presence in the area.
Developing a Guideline of Unmanned Aerial Vehicle’s Acquisition Geometry for Landslide Mapping and Monitoring
Authors: Nikolakopoulos Konstantinos G. , Kyriou Aggeliki , Koukouvelas Ioannis K.
Published: May 2022

Remote sensing data and techniques are widely used for monitoring and managing natural or man-made disasters, due to their timeliness and their satisfactory accuracy. A key stage in disaster research is the detailed and precise mapping of an affected area. The current work examines the relationship that may exist between the acquisition geometry of Unmanned Aerial Vehicle (UAV) campaigns and the topographic characteristics of an investigated area, toward landslide mapping and monitoring that is as accurate as possible. In fact, this work, concerning the systematic research of the acquisition geometry of UAV flights over multiple active landslides, is conducted for the first time and is focused on creating a guideline for any researcher trying to follow the UAV photogrammetric survey during landslide mapping and monitoring. In particular, UAV flights were executed over landslide areas with different characteristics (land cover, slope, etc.) and the collected data from each area were classified into three groups depending on UAV acquisition geometry, i.e., nadir imagery, oblique imagery, and an integration of nadir and oblique imagery. High-resolution orthophotos and Digital Surface Models (DSMs) emerged from the processing of the UAV imagery of each group through structure-from-motion photogrammetry (SfM). Accuracy assessment was carried out using quantitative and qualitative comparative approaches, such as root mean square error calculation, length comparison, and mean center estimation. The evaluation of the results revealed that there is a strong relationship between UAV acquisition geometry and landslide characteristics, which is evident in the accuracy of the generated photogrammetric products (orthophotos, DSMs). In addition, it was proved that the synergistic processing of nadir and oblique imagery increased overall centimeter accuracy.
Effects of Greening Areas and Water Bodies on Urban Microclimate in Wuhan—A Simulation Study Considering Prospective Planning
Authors: Deng Qinli , Zhou Zeng , Shan Xiaofang , Li Chuancheng , Liu Daoru
Published: May 2022

To alleviate the urban heat island effect and reduce the consumption of electricity and expenditure caused by active cooling devices on hot days, many cities in tropical and subtropical areas emphasize the utilization of urban greening areas in current and future urban planning. We utilized the weather research and forecasting model (WRF) to simulate and study the impact of different greening area rates on the urban microclimate in business, residential, and industrial areas in Wuhan city. Meanwhile, we proposed two efficiency coefficients to evaluate the variable cooling benefit of the improvement of the greening area. The results show that greening areas and water bodies are the cooling sources of cities and that industrial areas benefit the most from improvements in the greening rate, with the average temperature declining by 1.06 °C with a 20% increase in the greening rate, while the corresponding values of residential and industrial areas were 0.98 °C and 0.92 °C, respectively. This research provides a reference for the future planning of tropical and subtropical areas to help improve the urban microclimate, thermal environment, and environmental comfort on hot days.
CFD Study of Dry Pulmonary Surfactant Aerosols Deposition in Upper 17 Generations of Human Respiratory Tract
Authors: Gemci Tevfik , Ponyavin Valery , Collins Richard , Corcoran Timothy E. , Saha Suvash C. , Islam Mohammad S.
Published: May 2022

The efficient generation of high concentrations of fine-particle, pure surfactant aerosols provides the possibility of new, rapid, and effective treatment modalities for Acute Respiratory Distress Syndrome (ARDS). SUPRAER-CATM is a patented technology by Kaer BiotherapeuticsTM, which is a new class of efficient aerosol drug generation and delivery system using Compressor Air (CA). SUPRAER-CA is capable of aerosolizing relatively viscous solutions or suspensions of proteins and surfactants and of delivering them as pure fine particle dry aerosols. In this Computational Fluid Dynamics (CFD) study, we select a number of sites within the upper 17 generations of the human respiratory tract for calculation of the deposition of dry pulmonary surfactant aerosol particles. We predict the percentage of inhaled dry pulmonary surfactant aerosol arriving from the respiratory bronchioles to the terminal alveolar sacs. The dry pulmonary surfactant aerosols, with a Mass Median Aerodynamic Diameter (MMAD) of 2.6 µm and standard deviation of 1.9 µm, are injected into the respiratory tract at a dry surfactant aerosol flow rate of 163 mg/min to be used in the CFD study at an air inhalation flow rate of 44 L/min. This CFD study in the upper 17th generation of a male adult lung has shown computationally that the penetration fraction (PF) is approximately 25% for the inhaled surfactant aerosols. In conclusion, an ARDS patient might receive approximately one gram of inspired dry surfactant aerosol during an administration period of one hour as a possible means of further inflating partly collapsed alveoli.
Hydrological Impact of the New ECMWF Multi-Layer Snow Scheme
Authors: Zsoter Ervin , Arduini Gabriele , Prudhomme Christel , Stephens Elisabeth , Cloke Hannah
Published: May 2022

The representation of snow is a crucial aspect of land-surface modelling, as it has a strong influence on energy and water balances. Snow schemes with multiple layers have been shown to better describe the snowpack evolution and bring improvements to soil freezing and some hydrological processes. In this paper, the wider hydrological impact of the multi-layer snow scheme, implemented in the ECLand model, was analyzed globally on hundreds of catchments. ERA5-forced reanalysis simulations of ECLand were coupled to CaMa-Flood, as the hydrodynamic model to produce river discharge. Different sensitivity experiments were conducted to evaluate the impact of the ECLand snow and soil freezing scheme changes on the terrestrial hydrological processes, with particular focus on permafrost. It was found that the default multi-layer snow scheme can generally improve the river discharge simulation, with the exception of permafrost catchments, where snowmelt-driven floods are largely underestimated, due to the lack of surface runoff. It was also found that appropriate changes in the snow vertical discretization, destructive metamorphism, snow-soil thermal conductivity and soil freeze temperature could lead to large river discharge improvements in permafrost by adjusting the evolution of soil temperature, infiltration and the partitioning between surface and subsurface runoff.
Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages
Authors: Mandim Filipa , Petropoulos Spyridon A. , Pinela José , Dias Maria Inês , Kostic Marina , Soković Marina , Ferreira Isabel C. F. R. , Santos-Buelga Celestino , Barros Lillian
Published: May 2022

Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1–B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O-caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 µg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 µg/mL for Δt of 60 and 120 min, respectively) and higher cytotoxic (B8–B13, GI50 between 7.1 and 17 µg/mL), anti-inflammatory (B13, IC50 of 10 µg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.
Are There Betel Quid Mixtures Less Harmful than Others? A Scoping Review of the Association between Different Betel Quid Ingredients and the Risk of Oral Submucous Fibrosis
Authors: Cirillo Nicola , Duong Peter Hung , Er Wee Teng , Do Casey Thao Nhi , De Silva Manikkuwadura Eranda Harshan , Dong Yining , Cheong Sok Ching , Sari Elizabeth Fitriana , McCullough Michael J. , Zhang Pangzhen , Prime Stephen S.
Published: May 2022

Oral submucous fibrosis (OSF) is a potentially malignant condition of the oral cavity characterized by progressive fibrosis of the submucosal tissues. OSF is typically associated with the use of betel quid (BQ), a chewing package made of natural products (e.g., areca nut, betel leaves), with or without smokeless tobacco. BQ ingredients contain pro-carcinogenic bioactive compounds, but also potentially protective biomolecules, and we have shown recently that the chemical properties of different BQ recipes vary, which may explain the unequal prevalence of OSF and oral cancer in BQ users in different geographical regions. Hence, this scoping review was aimed at evaluating the existing literature regarding different BQ compounds and their association with OSF. The repository of the National Library of Medicine (MEDLINE/PubMed), medRxiv databases, Google scholar, Baidu scholar, CNKI, and EBSCO were used to search for publications that investigated the association between BQ chewing and OSF up to November 2021. The search terminology was constructed using the keywords “betel quid” and “oral submucous fibrosis”, and their associated terms, with the use of Boolean operators. The search was conducted under Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines, together with clear inclusion and exclusion criteria. The review showed that the risk of developing OSF varied between different BQ recipes, and that chewing BQ mixtures containing betel inflorescence (BI) significantly increased the risk of OSF, as did the addition of tobacco. Conversely, the use of betel leaf in the mixture was likely to be protective, which may be due to the presence of polyphenols. Although further research is needed to determine the effect of individual BQ ingredients in the development of OSF, our pilot results provide the scope and rationale for informing future chemopreventive strategies for OSF and oral cancer in BQ chewers.
Gene Polymorphism of Biotransformation Enzymes and Ciprofloxacin Pharmacokinetics in Pediatric Patients with Cystic Fibrosis
Authors: Zyryanov Sergey K. , Ushkalova Elena A. , Kondratyeva Elena I. , Butranova Olga I. , Kondakova Yulia A.
Published: May 2022

(1) Background: Ciprofloxacin (CPF) is widely used for the treatment of cystic fibrosis, including pediatric patients, but its pharmacokinetics is poorly studied in this population. Optimal CPF dosing in pediatric patients may be affected by gene polymorphism of the enzymes involved in its biotransformation. (2) Materials and Methods: a two-center prospective non-randomized study of CPF pharmacokinetics with sequential enrollment of patients (n-33, mean age 9.03 years, male-33.36%), over a period from 2016 to 2021. All patients received tablets of the original CPF drug Cyprobay® at a dose of 16.5 mg/kg to 28.80 mg/kg. Blood sampling schedule: 0 (before taking the drug), 1.5 h; 3.0 h; 4.5 h; 6.0 h; 7.5 h after the first dosing. CPF serum concentrations were analyzed by high performance liquid chromatography mass spectrometry. The genotype of biotransformation enzymes was studied using total DNA isolated from whole blood leukocytes by the standard method. (4) Results: a possible relationship between the CA genotype of the CYP2C9 gene (c.1075A > C), the GG genotype of the CYP2D6*4 gene (1846G > A), the AG genotype of the GSTP1 gene (c.313A > G), the GCLC* genotype 7/7 and the CPF concentration in plasma (increased value of the area under the concentration–time curve) was established. Conclusions: Gene polymorphism of biotransformation enzymes may affect ciprofloxacin pharmacokinetics in children.
Identification of the Key Genes and Potential Therapeutic Compounds for Abdominal Aortic Aneurysm Based on a Weighted Correlation Network Analysis
Authors: Li Lin , Kan Kejia , Pallavi Prama , Keese Michael
Published: May 2022

Background: There is still an unmet need for therapeutic drugs for patients with an abdominal aortic aneurysm (AAA), especially for candidates unsuitable for surgical or interventional repair. Therefore, the purpose of this in silico study is to identify significant genes and regulatory mechanisms in AAA patients to predicate the potential therapeutic compounds for significant genes. Methods: The GSE57691 dataset was obtained from Gene Expression Omnibus (GEO) and used to identify the differentially expressed genes (DEGs) and weighted correlation network analysis (WGCNA). The biological function of DEGs was determined using gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). AAA-related genes were obtained from the Comparative Toxicogenomics Database (CTD) using the keywords: aortic aneurysm and abdominal. The hub genes in AAA were obtained by overlapping DEGs, WGCNA-based hub genes, and CTD-based genes. The diagnostic values of hub genes were determined using ROC curve analysis. Hereby, a TF-miRNA-hub gene network was constructed based on the miRnet database. Using these data, potential therapeutic compounds for the therapy of AAA were predicted based on the Drug Gene Interaction Database (DGIdb). Results: A total of 218 DEGs (17 upregulated and 201 downregulated) and their biological function were explored; 4093 AAA-related genes were derived by text mining. Three hub modules and 144 hub genes were identified by WGCNA. asparagine synthetase (ASNS), axin-related protein 2 (AXIN2), melanoma cell adhesion molecule (MCAM), and the testis-specific Y-encoded-like protein 1 (TSPYL1) were obtained as intersecting hub genes and the diagnostic values were confirmed with ROC curves. As potential compounds targeting the hub genes, asparaginase was identified as the target compound for ASNS. Prednisolone and abiraterone were identified as compounds targeting TSPYL1. For MCAM and TSPYL1, no potential therapeutic compound could be predicted. Conclusion: Using WGCNA analysis and text mining, pre-existing gene expression data were used to provide novel insight into potential AAA-related protein targets. For two of these targets, compounds could be predicted.
Sex Differences in Substance Use, Prevalence, Pharmacological Therapy, and Mental Health in Adolescents with Attention-Deficit/Hyperactivity Disorder (ADHD)
Authors: Castellano-García Francisca , Benito Ana , Jovani Antonio , Fuertes-Sáiz Alejandro , Marí-Sanmillán María Isabel , Haro Gonzalo
Published: May 2022

Sex differences are poorly studied within the field of mental health, even though there is evidence of disparities (with respect to brain anatomy, activation patterns, and neurochemistry, etc.) that can significantly influence the etiology and course of mental disorders. The objective of this work was to review sex differences in adolescents (aged 13–18 years) diagnosed with ADHD (according to the DSM-IV, DSM-IV-TR and DSM-5 criteria) in terms of substance use disorder (SUD), prevalence, pharmacological therapy and mental health. We searched three academic databases (PubMed, Web of Science, and Scopus) and performed a narrative review of a total of 21 articles. The main conclusions of this research were (1) girls with ADHD are more at risk of substance use than boys, although there was no consensus on the prevalence of dual disorders; (2) girls are less frequently treated because of underdiagnosis and because they are more often inattentive and thereby show less disruptive behavior; (3) together with increased impairment in cognitive and executive functioning in girls, the aforementioned could be related to greater substance use and poorer functioning, especially in terms of more self-injurious behavior; and (4) early diagnosis and treatment of ADHD, especially in adolescent girls, is essential to prevent early substance use, the development of SUD, and suicidal behavior.