bluuebunny/crossref_metadata_2025 huggingface dataset:
Blockade of Intranigral and Systemic D3 Receptors Stimulates Motor Activity in the Rat Promoting a Reciprocal Interaction among Glutamate, Dopamine, and GABA
Authors: Rodríguez-Sánchez Marina , Escartín-Pérez Rodrigo Erick , Leyva-Gómez Gerardo , Avalos-Fuentes José Arturo , Paz-Bermúdez Francisco Javier , Loya-López Santiago Iván , Aceves Jorge , Erlij David , Cortés Hernán , Florán Benjamín
Published: September 2019

In vivo activation of dopamine D3 receptors (D3Rs) depresses motor activity. D3Rs are widely expressed in subthalamic, striatal, and dendritic dopaminergic inputs into the substantia nigra pars reticulata (SNr). In vitro studies showed that nigral D3Rs modulate their neurotransmitter release; thus, it could be that these changes in neurotransmitter levels modify the discharge of nigro-thalamic neurons and, therefore, motor behavior. To determine how the in vitro responses correspond to the in vivo responses, we examined the effect of intra-nigral and systemic blockade of D3Rs in the interstitial content of glutamate, dopamine, and GABA within the SNr using microdialysis coupled to motor activity determinations in freely moving rats. Intranigral unilateral blockade of D3R with GR 103,691 increased glutamate, dopamine, and GABA. Increments correlated with increased ambulatory distance, non-ambulatory activity, and induced contralateral turning. Concomitant blockade of D3R with D1R by perfusion of SCH 23390 reduced the increase of glutamate; prevented the increment of GABA, but not of dopamine; and abolished behavioral effects. Glutamate stimulates dopamine release by NMDA receptors, while blockade with kynurenic acid prevented the increase in dopamine and, in turn, of GABA and glutamate. Finally, systemic administration of D3R selective antagonist U 99194A increased glutamate, dopamine, and GABA in SNr and stimulated motor activity. Blockade of intra-nigral D1R with SCH 23390 prior to systemic U 99194A diminished increases in neurotransmitter levels and locomotor activity. These data highlight the pivotal role of presynaptic nigral D3 and D1R in the control of motor activity and help to explain part of the effects of the in vivo administration of D3R agents.
Regulation of Membrane Calcium Transport Proteins by the Surrounding Lipid Environment
Authors: Conrard Louise , Tyteca Donatienne
Published: September 2019

Calcium ions (Ca2+) are major messengers in cell signaling, impacting nearly every aspect of cellular life. Those signals are generated within a wide spatial and temporal range through a large variety of Ca2+ channels, pumps, and exchangers. More and more evidences suggest that Ca2+ exchanges are regulated by their surrounding lipid environment. In this review, we point out the technical challenges that are currently being overcome and those that still need to be defeated to analyze the Ca2+ transport protein–lipid interactions. We then provide evidences for the modulation of Ca2+ transport proteins by lipids, including cholesterol, acidic phospholipids, sphingolipids, and their metabolites. We also integrate documented mechanisms involved in the regulation of Ca2+ transport proteins by the lipid environment. Those include: (i) Direct interaction inside the protein with non-annular lipids; (ii) close interaction with the first shell of annular lipids; (iii) regulation of membrane biophysical properties (e.g., membrane lipid packing, thickness, and curvature) directly around the protein through annular lipids; and (iv) gathering and downstream signaling of several proteins inside lipid domains. We finally discuss recent reports supporting the related alteration of Ca2+ and lipids in different pathophysiological events and the possibility to target lipids in Ca2+-related diseases.
Novel Thiazole Phenoxypyridine Derivatives Protect Maize from Residual Pesticide Injury Caused by PPO-Inhibitor Fomesafen
Authors: Zhao Li-Xia , Yin Min-Lei , Wang Qing-Rui , Zou Yue-Li , Ren Tao , Gao Shuang , Fu Ying , Ye Fei
Published: September 2019

The herbicide fomesafen has the advantages of low toxicity and high selectivity, and the target of this compound is protoporphyrinogen IX oxidase (PPO, EC 1.3.3.4). However, this herbicide has a long residual period and can have phytotoxic effects on succeeding crops. To protect maize from fomesafen, a series of thiazole phenoxypyridines were designed based on structure–activity relationships, active substructure combinations, and bioisosterism. Bioassays showed that thiazole phenoxypyridines could improve maize tolerance under fomesafen toxicity stress to varying degrees at a dose of 10 mg·kg−1. Compound 4i exhibited the best effects. After being treated by compound 4i, average recovery rates of growth index exceeded 72%, glutathione content markedly increased by 167% and glutathione S-transferase activity was almost 163% of fomesafen-treated group. More importantly, after being treated by compound 4i, the activity of PPO, the main target enzyme of fomesafen, recovered to 93% of the control level. The molecular docking result exhibited that the compound 4i could compete with fomesafen to bind with the herbicide target enzyme, which consequently attained the herbicide detoxification. The present work suggests that compound 4i could be developed as a potential safener to protect maize from fomesafen.
Using an Overlapping Time Interval Strategy to Study Diagnostic Instability in Mild Cognitive Impairment Subtypes
Authors: Facal David , Guàrdia-Olmos Joan , Pereiro Arturo X. , Lojo-Seoane Cristina , Peró Maribel , Juncos-Rabadán Onésimo
Published: September 2019

(1) Background: Mild cognitive impairment (MCI) is a diagnostic label in which stability is typically low. The aim of this study was to examine temporal changes in the diagnosis of MCI subtypes by using an overlapping-time strategy; (2) Methods: The study included 435 participants aged over 50 years with subjective cognitive complaints and who completed at least one follow-up evaluation. The probability of transition was estimated using Bayesian odds ratios; (3) Results: Within the different time intervals, the controls with subjective cognitive complaints represented the largest proportion of participants, followed by sda-MCI at baseline and in the first five intervals of the follow-up, but not in the last eight intervals. The odds ratios indicated higher odds of conversion to dementia in sda-MCI and mda-MCI groups relative to na-MCI (e.g., interval 9–15 months—sda-MCI OR = 9 and mda-MCI OR = 3.36; interval 27–33—sda-MCI OR = 16 and mda-MCI = 5.06; interval 42–48—sda-MCI OR = 8.16 and mda-MCI = 3.45; interval 45–51—sda-MCI OR = 3.31 and mda-MCI = 1); (4) Conclusions: Notable patterns of instability consistent with the current literature were observed. The limitations of a prospective approach in the study of MCI transitions are discussed.
The Emerging Roles of Cancer Stem Cells and Wnt/Beta-Catenin Signaling in Hepatoblastoma
Authors: Mavila Nirmala , Thundimadathil Jyothi
Published: September 2019

Hepatoblastoma (HB) is the most common form of primary liver malignancy found in pediatric populations. HB is considered to be clonal and arises from hepatoblasts, or embryonic liver progenitor cells. These less differentiated tumor-initiating progenitor cells, or cancer stem cells (CSCs), may contribute to tumor recurrence and resistance to therapies, and have high metastatic abilities. Phenotypic heterogeneity, undesired genetic and epigenetic alterations, and dysregulated signaling pathways provide CSCs with a survival advantage over current therapies. The molecular and cellular basis of HB and the mechanism of CSC induction are not fully understood. The Wnt/beta-catenin pathway is one of the major developmental pathways and is believed to play an important role in the pathogenesis of HB and CSC formation. This review summarizes the cellular and molecular characteristics of HB with a specific emphasis on CSCs and Wnt/beta-catenin signaling.
Methylation Assessment for the Prediction of Malignancy in Mediastinal Adenopathies Obtained by Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration in Patients with Lung Cancer
Authors: Leiro-Fernandez Virginia , De Chiara Loretta , Rodríguez-Girondo Mar , Botana-Rial Maribel , Valverde Diana , Núñez-Delgado Manuel , Fernández-Villar Alberto
Published: September 2019

The evaluation of mediastinal lymph nodes is critical for the correct staging of patients with lung cancer (LC). Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a minimally invasive technique for mediastinal staging, though unfortunately lymph node micrometastasis is often missed by cytological analysis. The aim of this study was to evaluate the predictive capacity of methylation biomarkers and provide a classification rule for predicting malignancy in false negative EBUS-TBNA samples. The study included 112 patients with a new or suspected diagnosis of LC that were referred to EBUS-TBNA. Methylation of p16/INK4a, MGMT, SHOX2, E-cadherin, DLEC1, and RASSF1A was quantified by nested methylation-specific qPCR in 218 EBUS-TBNA lymph node samples. Cross-validated linear regression models were evaluated to predict malignancy. According to EBUS-TBNA and final diagnosis, 90 samples were true positives for malignancy, 110 were true negatives, and 18 were false negatives. MGMT, SHOX2, and E-cadherin were the methylation markers that better predicted malignancy. The model including sex, age, short axis diameter and standard uptake value of adenopathy, and SHOX2 showed 82.7% cross-validated sensitivity and 82.4% specificity for the detection of malignant lymphadenopathies among negative cytology samples. Our results suggest that the predictive model approach proposed can complement EBUS-TBNA for mediastinal staging.
Age-Related Alterations Affecting the Chondrogenic Differentiation of Synovial Fluid Mesenchymal Stromal Cells in an Equine Model
Authors: Mazzotti Eleonora , Teti Gabriella , Falconi Mirella , Chiarini Francesca , Barboni Barbara , Mazzotti Antonio , Muttini Aurelio
Published: September 2019

Osteoarthritis is a degenerative disease that strongly correlates with age and promotes the breakdown of joint cartilage and subchondral bone. There has been a surge of interest in developing cell-based therapies, focused particularly on the use of mesenchymal stromal cells (MSCs) isolated from adult tissues. It seems that MSCs derived from synovial joint tissues exhibit superior chondrogenic ability, but their unclear distribution and low frequency actually limit their clinical application. To date, the influence of aging on synovial joint derived MSCs’ biological characteristics and differentiation abilities remains unknown, and a full understanding of the mechanisms involved in cellular aging is lacking. The aim of this study was therefore to investigate the presence of age-related alterations in synovial fluid MSCs and their influence on the potential ability of MSCs to differentiate toward chondrogenic phenotypes. Synovial fluid MSCs, isolated from healthy equine donors from 3 to 40 years old, were cultured in vitro and stimulated towards chondrogenic differentiation for up to 21 days. An equine model was chosen due to the high degree of similarity of the anatomy of the knee joint to the human knee joint and as spontaneous disorders develop that are clinically relevant to similar human disorders. The results showed a reduction in cell proliferation correlated with age and the presence of age-related tetraploid cells. Ultrastructural analysis demonstrated the presence of morphological features correlated with aging such as endoplasmic reticulum stress, autophagy, and mitophagy. Alcian blue assay and real-time PCR data showed a reduction of efficiency in the chondrogenic differentiation of aged synovial fluid MSCs compared to young MSCs. All these data highlighted the influence of aging on MSCs’ characteristics and ability to differentiate towards chondrogenic differentiation and emphasize the importance of considering age-related alterations of MSCs in clinical applications.
The E-Cadherin and N-Cadherin Switch in Epithelial-to-Mesenchymal Transition: Signaling, Therapeutic Implications, and Challenges
Authors: Loh Chin-Yap , Chai Jian , Tang Ting , Wong Won , Sethi Gautam , Shanmugam Muthu , Chong Pei , Looi Chung
Published: September 2019

Epithelial-to-Mesenchymal Transition (EMT) has been shown to be crucial in tumorigenesis where the EMT program enhances metastasis, chemoresistance and tumor stemness. Due to its emerging role as a pivotal driver of tumorigenesis, targeting EMT is of great therapeutic interest in counteracting metastasis and chemoresistance in cancer patients. The hallmark of EMT is the upregulation of N-cadherin followed by the downregulation of E-cadherin, and this process is regulated by a complex network of signaling pathways and transcription factors. In this review, we summarized the recent understanding of the roles of E- and N-cadherins in cancer invasion and metastasis as well as the crosstalk with other signaling pathways involved in EMT. We also highlighted a few natural compounds with potential anti-EMT property and outlined the future directions in the development of novel intervention in human cancer treatments. We have reviewed 287 published papers related to this topic and identified some of the challenges faced in translating the discovery work from bench to bedside.
Social Cohesion and City Green Space: Revisiting the Power of Volunteering
Authors: Jennings Viniece
Published: September 2019

Urban green spaces can facilitate positive social interactions in numerous ways. These interactions may lead to the development of social cohesion among city dwellers. This article provides a viewpoint on the potential role that the presence of green space and volunteering can play in the construct of social cohesion. Specifically, the article focuses on one aspect of social cohesion, volunteerism, as a way to illustrate the immense potential of social cohesion to link health with place and the planet.
Preparation and Characterization of Polydopamine-Modified Ni/Carbon Nanotubes Friction Composite Coating
Authors: Wang Ye , Chen Jianfeng
Published: September 2019

In this paper, dopamine (DA) was used for the surface modification of carbon nanotubes (CNTs) to solve the agglomeration problem. High resolution transmission electron microscopy (HRTEM) and X-ray photoelectron spectroscopy (XPS) were utilized to understand the morphology and presence of different elements in the modified CNTs. The treated CNT surfaces were wrapped by a pDA layer and the layer thickness was controlled by reaction time. The dispersion test showed that the treated CNTs remained uniformly dispersed in solution for more than 30 days. The Ni/CNTs@pDA composite coating on the mild steel specimen was obtained by the electrodeposition technique. Ball-on-disc friction-wear equipment and an electrochemical workstation were used to test the friction-wear and corrosion resistance of the composite coating. The results indicated that the Ni/CNTs@pDA coating showed excellent tribological performance and were superior to the pure Ni coating.
Growth and Strain Engineering of Trigonal Te for Topological Quantum Phases in Non-Symmorphic Chiral Crystals
Authors: Basnet Rabindra , Doha M. , Hironaka Takayuki , Pandey Krishna , Davari Shiva , Welch Katie , Churchill Hugh , Hu Jin
Published: September 2019

Strained trigonal Te has been predicted to host Weyl nodes supported by a non-symmorphic chiral symmetry. Using low-pressure physical vapor deposition, we systematically explored the growth of trigonal Te nanowires with naturally occurring strain caused by curvature of the wires. Raman spectra and high mobility electronic transport attest to the highly crystalline nature of the wires. Comparison of Raman spectra for both straight and curved nanowires indicates a breathing mode that is significantly broader and shifted in frequency for the curved wires. Strain induced by curvature during growth therefore may provide a simple pathway to investigate topological phases in trigonal Te.
Genetic Characterization of Cleveland Bay Horse Breed
Authors: Khanshour Anas M. , Hempsey Eleanore K. , Juras Rytis , Cothran E. Gus
Published: September 2019

The Cleveland Bay (CB) is the United Kingdom’s oldest established horse breed. In this study we analyzed the genetic variability in CB horses and investigated its genetic relationships with other horse breeds. We examined the genetic variability among 90 CB horses sampled in the USA compared to a total of 3447 horses from 59 other breeds. Analysis of the genetic diversity and population structure was carried out using 15 microsatellite loci. We found that genetic diversity in CB horses was less than that for the majority of other tested breeds. The genetic similarity measures showed no direct relationship between the CB and Thoroughbred but suggested the Turkman horses (likely in the lineage of ancestors of the Thoroughbred) as a possible ancestor. Our findings reveal the genetic uniqueness of the CB breed and indicate its need to be preserved as a genetic resource.
The Landauer Principle: Re-Formulation of the Second Thermodynamics Law or a Step to Great Unification?
Authors: Bormashenko Edward
Published: September 2019

The Landauer principle quantifies the thermodynamic cost of the recording/erasure of one bit of information, as it was stated by its author: “information is physical” and it has an energy equivalent. In its narrow sense, the Landauer principle states that the erasure of one bit of information requires a minimum energy cost equal to kBT ln2, where T is the temperature of a thermal reservoir used in the process and k B is Boltzmann’s constant. The Landauer principle remains highly debatable. It has been argued that, since it is not independent of the second law of thermodynamics, it is either unnecessary or insufficient as an exorcism of Maxwell’s demon. On the other hand, the Landauer principle enables the “informational” reformulation of thermodynamic laws. Thus, the Landauer principle touches the deepest physical roots of thermodynamics. Authors are invited to contribute papers devoted to the meaning, interpretation, physical roots, experimental verification and applications of the Landauer principle. Papers devoted to the quantum and relativity aspects of the Landauer principle are encouraged.
Heat Transfer Performance in a Superheater of an Industrial CFBC Using Fuzzy Logic-Based Methods
Authors: Krzywanski Jaroslaw
Published: September 2019

The heat transfer coefficient in the combustion chamber of industrial circulating flidized bed (CFB) boilers depends on many parameters as it is a result of multifactorial mechanisms proceeding in the furnace. Therefore, the development of an effective modeling tool, which allows for predicting the heat transfer coefficient is interesting as well as a timely subject, of high practical significance. The present paper deals with an innovative application of fuzzy logic-based (FL) method for the prediction of a heat transfer coefficient for superheaters of fluidized-bed boilers, especially circulating fluidized-bed combustors (CFBC). The approach deals with the modeling of heat transfer for the Omega Superheater, incorporated into the reaction chamber of an industrial 670 t/h CFBC. The height above the grid, bed temperature and voidage, gas velocity, and the boiler’s load constitute inputs. The developed Fuzzy Logic Heat (FLHeat) model predicts the local overall heat transfer coefficient of the Omega Superheater. The model is in good agreement with the measured data. The highest overall heat transfer coefficient is equal 220 W/(m2K) and can be achieved by the SH I superheater for the following inputs l = 20 m, tb = 900 °C, v = 0.95, u = 7 m/s, M-C-R = 100%. The proposed technique is an effective strategy and an option for other procedures of heat transfer coefficient evaluation.
Approaching Modern Monetary Theory with a Taylor Rule
Authors: Mattson Ryan S. , Pjesky Rex
Published: September 2019

Considering the goals of Modern Monetary Theorists, this article examines inflation stabilization and employment maximization through a Taylor Rule for fiscal policy, similar to John Taylor’s foundational examination of the behavior of the Federal Reserve. If it is the role of the federal government to aid in the maintenance of the dual mandate of the Federal Reserve, then their behavior should follow a similar policy of setting an intermediate target of deficits relative to the maximum employment (the “Federal Job Guarantee”) and the inflation target. The paper will compare the historical data with the rule. When the predictions of the Deficit Rule are compared to historical data from 1965, we find that fiscal policy aligns with what the Deficit Rule predicts with two exceptions: the stagflation of the 1970s and the current increases in budget deficits.
Mechanical Anisotropy and Failure Characteristics of Columnar Jointed Rock Masses (CJRM) in Baihetan Hydropower Station: Structural Considerations Based on Digital Image Processing Technology
Authors: Xia Yingjie , Zhang Chuanqing , Zhou Hui , Zhang Chunsheng , Hong Wangbing
Published: September 2019

The columnar joints in Baihetan hydropower station are primary tensile joints since they were formed during the process of lava condensation. Understanding the influence of columnar jointed rock mass (CJRM) on the mechanical response and failure modes is the basis for designing of associated engineering works. Hence, the structural characteristics of Baihetan CJRM were analyzed by carrying out a geological survey at first. Three groups of numerical models capable of reflecting the structural characteristics of CJRM were then established to analyze the mechanical and failure characteristics. The results in this study showed that: (1) Irregularity of columnar basalt restricted crack propagation on columnar joints and also led to stress concentration in the distorted parts, and thus, damage of basalt columns; (2) when the included angle between direction of concentrated defect structures in CJRM and uniaxial stress was large, the defect structures can prevent crack propagation on columnar joints, and the failure of defect structure can cause the overall failure of the rock mass; and (3) under the condition of same columnar structure and included angle, the peak strength of models with microcracks and structural plane was low and the irregular shape of columnar joints decreased the anisotropy of mechanical parameters.
Bridging the Gap between Automated Manufacturing of Fuel Cell Components and Robotic Assembly of Fuel Cell Stacks
Authors: Fowler Devin , Gurau Vladimir , Cox Daniel
Published: September 2019

Recently demonstrated robotic assembling technologies for fuel cell stacks used fuel cell components manually pre-arranged in stacks (presenters). Identifying the original orientation of fuel cell components and loading them in presenters for a subsequent automated assembly process is a difficult, repetitive work cycle which if done manually, deceives the advantages offered by either the automated fabrication technologies for fuel cell components or by the robotic assembly processes. We present for the first time a robotic technology which enables the integration of automated fabrication processes for fuel cell components with a robotic assembly process of fuel cell stacks into a fully automated fuel cell manufacturing line. This task uses a Yaskawa Motoman SDA5F dual arm robot with integrated machine vision system. The process is used to identify and grasp randomly placed, slightly asymmetric fuel cell components, to reorient them all in the same position and stack them in presenters in preparation for a subsequent robotic assembly process. The process was demonstrated as part of a larger endeavor of bringing to readiness advanced manufacturing technologies for alternative energy systems, and responds the high priority needs identified by the U.S. Department of Energy for fuel cells manufacturing research and development.
Ammonia, Hydrogen Sulfide, and Greenhouse Gas Emissions from Lab-Scaled Manure Bedpacks with and without Aluminum Sulfate Additions
Authors: Spiehs Mindy J. , Woodbury Bryan L. , Parker David B.
Published: September 2019

The poultry industry has successfully used aluminum sulfate (alum) as a litter amendment to reduce NH3 emissions from poultry barns, but alum has not been evaluated for similar uses in cattle facilities. A study was conducted to measure ammonia (NH3), greenhouse gases (GHG), and hydrogen sulfide (H2S) emissions from lab-scaled bedded manure packs over a 42-day period. Two frequencies of application (once or weekly) and four concentrations of alum (0, 2.5, 5, and 10% by mass) were evaluated. Frequency of alum application was either the entire treatment of alum applied on Day 0 (once) or 16.6% of the total alum mass applied each week for six weeks. Ammonia emissions were reduced when 10% alum was used, but H2S emissions increased as the concentration of alum increased in the bedded packs. Nitrous oxide emissions were not affected by alum treatment. Methane emissions increased as the concentration of alum increased in the bedded packs. Carbon dioxide emissions were highest when 5% alum was applied and lowest when 0% alum was used. Results of this study indicate that 10% alum is needed to effectively reduce NH3 emissions, but H2S and methane emissions may increase when this concentration of alum is used.
Assessing Vegetation Composition and the Indicator Species around Water Source Areas in a Pine Forest Plantation: A Case Study from Watujali and Silengkong Catchments, Kebumen, Indonesia
Authors: Siswo  , Yun Chung-Weon , Abdiyani Susi
Published: September 2019

This research aimed to assess vegetation composition and the indicator species around water source areas of pine forest plantation. Data were collected through interview and vegetation survey. Vegetation communities were first compared using multi-response permutation procedure (MRPP) analysis. Indicator species analysis was then employed to determine the indicator species for each condition by considering historical data from the interview. Canonical correspondence analysis (CCA) and simple correlation analysis were also included. The result showed significant differences in species composition between water source areas in Watujali (lower low flow) and Silengkong (higher low flow) catchments, indicated by T = −5.104, p = 0.000. Pinus merkusii was dominant in Watujali (important value = 78%, D′ = 0.62) compared to Silengkong (important value = 41%, D′ = 0.21), and in becoming an indicator species (value = 52.1, p = 0.042) for Watujali. Meanwhile, Laportea sinuata, as the specific tree of water source areas, was an indicator for Silengkong (value = 29.4, p = 0.004). At a smaller level, indicator species differentiated the two catchments, even though they shared similar in D′ and H′. Among specific plants of water source areas, only Ficus septica was an indicator for Watujali (value = 29.4, p = 0.004), given its adaptability. Specific plants of water source areas, including Laportea sinuata, Coctus spicatus, and Calocassia gigantea, were significant indicators for Silengkong catchments, illustrated by 34.6, 35.9, and 33.0 of indicator values, respectively. These results also reflected the relationship among tree vegetation change, environmental features, and the growth of smaller species, as implied by both CCA and simple correlation. This finding could be used as basic information for early assessment of environmental change and environmental restoration efforts around water source areas on pine forest plantations. Repetition of this study is suggested to be carried out in other pine forest areas, as each region sometimes has its own specific native and natural species.
Effects of Thinning on Microbial Community Structure in the Organic Horizon of Chinese Pine Plantations in Badaling, Beijing, China
Authors: Wang Ling , Zhao Guangliang , Sun Yujun , Yang Ying , Zhou Hongjuan
Published: September 2019

Research Highlights: Moderate thinning can effectively improve forestry production and change the microenvironment of understory vegetation. Background and Objectives: Microbial communities control the decomposition and transformation of forest organic matter; however, the influence of thinning on microbes in the organic horizon remains unclear. Materials and Methods: In this study, we subjected four plots of Chinese pine plantations in Badaling, Beijing to different thinning intensities, including no thinning (T0), low-intensity thinning (T10), medium-intensity thinning (T20), and high-intensity thinning (T50). The changes in chemical properties and microbial community compositions observed in the organic horizon, which comprised undecomposed litter (L layer) and half-decomposed litter (F layer), were analyzed after thinning. Microbial community compositions were evaluated using phospholipid fatty acid (PLFA) methods. Results: The results showed that the abundances of gram-negative bacteria (GN) and total bacteria (B) under the T10 thinning condition were the highest among the four thinning intensities, and the abundance of arbuscular mycorrhizal fungi (AMF) in T20 was higher than under other thinning intensities. The abundance of gram-positive bacteria (GP) and actinobacteria (ACT) in T10 was lower than in both T0 and T50. The abundance of total PLFAs and fungi (FU) was higher in the L layer, whereas the abundance of GP, GN, B, ACT, and AMF was higher in the F layer. Conclusions: Our results demonstrated that the L layer better reflects the influence of thinning on litter. Redundancy analysis (RDA) results indicated that the organic carbon (LOC), dissolved organic carbon (DOC), and ammonium nitrogen (NH4+-N)contents of litter were primarily responsible for the observed changes in microbial community structure, with LOC alone explaining 62.6% of the total variance among the litter substrate factors selected. Overall, moderate-intensity thinning of Pinus tabulaeformis Carr. plantations created more favorable conditions for microbial communities in the organic horizon.
Time and Power Allocation for Energy Efficiency Maximization in Wireless-Powered Full-Duplex Relay Systems
Authors: Song Xin , Ni Yue , Han Xiuwei , Qin Lei , Dong Li
Published: September 2019

In this paper, we propose an optimal time and power allocation scheme in a wireless power supply full-duplex (FD) relay system, where we consider the number of relay antennas in the energy harvesting stage. At the same time, the energy efficiency optimization problem of the system is structured, where optimization issues related to time allocation factors and power allocation are established. For the FD dual-antenna and the FD single-antenna energy harvesting system, energy efficiency function is proven to be a concave function over the time-switch factor, and the optimal time-switching factor is theoretically obtained using the Lambert function. Then, according to the given value range of the optimal time switching factor, the optimal power distribution scheme is obtained by analyzing the derivative function of the system energy efficiency and using the properties of the Lambert function. The time-switching factor and transmission power are optimally selected at the wireless power supply FD relay. Results reveal that the performance of energy efficiency of the dual-antenna energy harvesting at the FD relay outperforms that of the single-antenna. Moreover, our results demonstrate that FD relay systems always substantially boost the energy efficiency compared with half-duplex (HD) relay systems.
Genome-Wide Analysis of Cotton Auxin Early Response Gene Families and Their Roles in Somatic Embryogenesis
Authors: Sun Ruibin , Wang Shaohui , Ma Dan , Li Yilin , Liu Chuanliang
Published: September 2019

Auxin is well known to regulate growth and development processes. Auxin early response genes serve as a critical component of auxin signaling and mediate auxin regulation of diverse physiological processes. In the present study, a genome-wide identification and comprehensive analysis of auxin early response genes were conducted in upland cotton. A total of 71 auxin response factor (ARF), 86 Auxin/Indole-3-Acetic Acid (Aux/IAA), 63 Gretchen Hagen3 (GH3), and 194 small auxin upregulated RNA (SAUR) genes were identified in upland cotton, respectively. Phylogenetic analysis revealed that the ARF, GH3, and SAUR families were likely subject to extensive evolutionary divergence between Arabidopsis and upland cotton, while the Aux/IAA family was evolutionary conserved. Expression profiles showed that the ARF, Aux/IAA, GH3, and SAUR family genes were extensively involved in embryogenic competence acquisition of upland cotton callus. The Aux/IAA family genes generally showed a higher expression level in the non-embryogenic callus (NEC) of highly embryogenic cultivar CCRI24 than that of recalcitrant cultivar CCRI12, which may be conducive to initializing the embryogenic transformation. Auxin early response genes were tightly co-expressed with most of the known somatic embryogenesis (SE) related genes, indicating that these genes may regulate upland cotton SE by interacting with auxin early response genes.
Designing Activating Schoolyards: Seen from the Girls’ Viewpoint
Authors: Pawlowski Charlotte S. , Veitch Jenny , Andersen Henriette B. , Ridgers Nicola D.
Published: September 2019

Girls are typically less active in the schoolyard during recess than boys. It is therefore necessary to understand influences on girls’ recess activity in schoolyards. The aim of this qualitative study was to investigate girls’ perceptions of physical environmental factors influencing recess physical activity in re-designed schoolyards and to compare the perceptions of girls from different age groups. In 2018, 50 girls from five Danish schools were interviewed using photo-elicitation. The girls were from Grade 4 (n = 28, age 10–11) and Grade 6 (n = 22, age 12–13). Data were analysed using pen profiles constructed from verbatim transcripts. Ten factors emerged: variety, accessibility, size, designated spaces, greenery, playground markings, active play facilities, sports facilities, play equipment, and speakers. Play facilities (trampolines, obstacle courses, dancing and gymnastic appliances) were favoured over traditional sport facilities. Designated spaces, greenery and speakers were important for feeling comfortable within the schoolyard. Although similar factors were raised by the two age groups, some factors were perceived as enablers by the youngest and as barriers by the oldest girls, highlighting the complexity of designing schoolyards that cater to all ages. A greater understanding of how different designs and facilities may be perceived by girls of different ages is important for the design of future schoolyards.
Exploring the Experiences of West African Immigrants Living with Type 2 Diabetes in the UK
Authors: Alloh Folashade , Hemingway Ann , Turner-Wilson Angela
Published: September 2019

The increasing prevalence and poorer management of Type 2 diabetes among West African immigrants in the UK is a public health concern. This research explored the experiences of West African immigrants in the management of Type 2 diabetes in the UK using a constructivist grounded theory approach. In-depth individual interviews were conducted with thirty-four West African immigrants living with Type 2 diabetes in the London area. Fifteen male and nineteen female adult West African immigrants with age range from 33–82 years participated in the study. Participants were recruited from five diabetes support groups and community settings. Initial, focused and theoretical coding, constant comparison and memos were used to analyse collected data. Three concepts emerged: Changing dietary habits composed of participants’ experiences in meeting dietary recommendations, improving physical activity concerned with the experience of reduced physical activity since moving to the UK and striving to adapt which focus on the impact of migration changes in living with Type 2 diabetes in the UK. These address challenges that West African immigrants experience in the management of Type 2 diabetes in the UK. The findings of this research provide a better understanding of the influencing factors and can be used to improve the support provided for West Africans living with Type 2 diabetes in the UK, presenting a deeper understanding of socio-cultural factors that contribute to supporting individuals from this population.
Spatiotemporal Variability and Influencing Factors of Aerosol Optical Depth over the Pan Yangtze River Delta during the 2014–2017 Period
Authors: Cheng Liang , Li Long , Chen Longqian , Hu Sai , Yuan Lina , Liu Yunqiang , Cui Yifan , Zhang Ting
Published: September 2019

Large amounts of aerosol particles suspended in the atmosphere pose a serious challenge to the climate and human health. In this study, we produced a dataset through merging the Moderate Resolution Imaging Spectrometers (MODIS) Collection 6.1 3-km resolution Dark Target aerosol optical depth (DT AOD) with the 10-km resolution Deep Blue aerosol optical depth (DB AOD) data by linear regression and made use of it to unravel the spatiotemporal characteristics of aerosols over the Pan Yangtze River Delta (PYRD) region from 2014 to 2017. Then, the geographical detector method and multiple linear regression analysis were employed to investigate the contributions of influencing factors. Results indicate that: (1) compared to the original Terra DT and Aqua DT AOD data, the average daily spatial coverage of the merged AOD data increased by 94% and 132%, respectively; (2) the values of four-year average AOD were high in the north-east and low in the south-west of the PYRD; (3) the annual average AOD showed a decreasing trend from 2014 to 2017 while the seasonal average AOD reached its maximum in spring; and that (4) Digital Elevation Model (DEM) and slope contributed most to the spatial distribution of AOD, followed by precipitation and population density. Our study highlights the spatiotemporal variability of aerosol optical depth and the contributions of different factors over this large geographical area in the four-year period, and can, therefore, provide useful insights into the air pollution control for decision makers.
“Who Doesn’t Think about Technology When Designing Urban Environments for Older People?” A Case Study Approach to a Proposed Extension of the WHO’s Age-Friendly Cities Model
Authors: Marston Hannah R. , van Hoof Joost
Published: September 2019

The World Health Organization (WHO) strives to assist and inspire cities to become more “age-friendly”, and the fundamentals are included in the Global Age-Friendly Cities Guide. An age-friendly city enables residents to grow older actively within their families, neighbourhoods and civil society, and offers extensive opportunities for the participation of older people in the community. Over the decades, technology has become essential for contemporary and future societies, and even more imperative as the decades move on, given we are nearly in our third decade of the twenty-first century. Yet, technology is not explicitly considered in the 8-domain model by the WHO, which describes an age-friendly city. This paper discusses the gaps in the WHO’s age-friendly cities model in the field of technology and provides insights and recommendations for expansion of the model for application in the context of countries with a high human development index that wish to be fully age-friendly. This work is distinctive because of the proposed new age-friendly framework, and the work presented in this paper contributes to the fields of gerontology, geography urban and development, computer science, and gerontechnology.
The Roles of Sex Hormones in the Course of Atopic Dermatitis
Authors: Kanda Naoko , Hoashi Toshihiko , Saeki Hidehisa
Published: September 2019

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by T helper 2 cell (Th2)-shifted abnormal immunity, skin barrier impairment, and pruritus. The prevalence of AD in childhood is slightly higher in boys than in girls; after puberty, the sexual difference is reversed. The female preponderance in all generations exists in intrinsic AD with enhanced Th1 activity and nickel allergy, lacking increased serum IgE or filaggrin mutation. AD is often deteriorated before menstruation. We review the effects of sex hormones on immune responses and skin permeability barrier and propose possible hypotheses for the above phenomena. After puberty, the immune responses of patients are remarkably influenced by sex hormones. Estrogen and progesterone enhance the activities of Th2/regulatory T cell (Treg) but suppress Th1/Th17. Androgens suppress Th1/Th2/Th17 and induce Treg. The skin permeability barrier is fortified by estrogen but is impaired by progesterone and androgens. Dehydroepiandrosterone suppresses Th2 but enhances Th1. The amount of steroid sulfatase converting dehydroepiandrosterone sulfate to dehydroepiandrosterone is higher in women than in men, and thus, women might be more susceptible to the influence of dehydroepiandrosterone. The balance of modulatory effects of sex hormones on immune responses and skin barrier might regulate the course of AD.
Unravelling the Metabolic and Hormonal Machinery During Key Steps of Somatic Embryogenesis: A Case Study in Coffee
Authors: Awada Rayan , Campa Claudine , Gibault Estelle , Déchamp Eveline , Georget Frédéric , Lepelley Maud , Abdallah Cécile , Erban Alexander , Martinez-Seidel Federico , Kopka Joachim , Legendre Laurent , Léran Sophie , Conéjéro Geneviève , Verdeil Jean-Luc , Crouzillat Dominique , Breton David , Bertrand Benoît , Etienne Hervé
Published: September 2019

Somatic embryogenesis (SE) is one of the most promising processes for large-scale dissemination of elite varieties. However, for many plant species, optimizing SE protocols still relies on a trial-and-error approach. Using coffee as a model plant, we report here the first global analysis of metabolome and hormone dynamics aiming to unravel mechanisms regulating cell fate and totipotency. Sampling from leaf explant dedifferentiation until embryo development covered 15 key stages. An in-depth statistical analysis performed on 104 metabolites revealed that massive re-configuration of metabolic pathways induced SE. During initial dedifferentiation, a sharp decrease in phenolic compounds and caffeine levels was also observed while auxins, cytokinins and ethylene levels were at their highest. Totipotency reached its highest expression during the callus stages when a shut-off in hormonal and metabolic pathways related to sugar and energetic substance hydrolysis was evidenced. Abscisic acid, leucine, maltotriose, myo-inositol, proline, tricarboxylic acid cycle metabolites and zeatin appeared as key metabolic markers of the embryogenic capacity. Combining metabolomics with multiphoton microscopy led to the identification of chlorogenic acids as markers of embryo redifferentiation. The present analysis shows that metabolite fingerprints are signatures of cell fate and represent a starting point for optimizing SE protocols in a rational way.
Natural Products as Modulators of the Proteostasis Machinery: Implications in Neurodegenerative Diseases
Authors: Cuanalo-Contreras Karina , Moreno-Gonzalez Ines
Published: September 2019

Proteins play crucial and diverse roles within the cell. To exert their biological function they must fold to acquire an appropriate three-dimensional conformation. Once their function is fulfilled, they need to be properly degraded to hamper any possible damage. Protein homeostasis or proteostasis comprises a complex interconnected network that regulates different steps of the protein quality control, from synthesis and folding, to degradation. Due to the primary role of proteins in cellular function, the integrity of this network is critical to assure functionality and health across lifespan. Proteostasis failure has been reported in the context of aging and neurodegeneration, such as Alzheimer’s and Parkinson’s disease. Therefore, targeting the proteostasis elements emerges as a promising neuroprotective therapeutic approach to prevent or ameliorate the progression of these disorders. A variety of natural products are known to be neuroprotective by protein homeostasis interaction. In this review, we will focus on the current knowledge regarding the use of natural products as modulators of different components of the proteostasis machinery within the framework of age-associated neurodegenerative diseases.
Activation of the Sphingosine 1 Phosphate–Rho Pathway in Pterygium and in Ultraviolet-Irradiated Normal Conjunctiva
Authors: Igarashi Nozomi , Honjo Megumi , Fujishiro Takashi , Toyono Tetsuya , Ono Takashi , Mori Yosai , Miyata Kazunori , Obinata Hideru , Aihara Makoto
Published: September 2019

Sphingosine 1 phosphate (S1P) is a bioactive lipid that regulates cellular activity, including proliferation, cytoskeletal organization, migration, and fibrosis. In this study, the potential relevance of S1P–Rho signaling in pterygium formation and the effects of ultraviolet (UV) irradiation on activation of the S1P/S1P receptor axis and fibrotic responses were investigated in vitro. Expressions of the S1P2, S1P4, and S1P5 receptors were significantly higher in pterygium tissue than in normal conjunctiva, and the concentration of S1P was significantly elevated in the lysate of normal conjunctival fibroblast cell (NCFC) irradiated with UV (UV-NCFCs). RhoA activity was significantly upregulated in pterygium fibroblast cells (PFCs) and UV-NCFCs, and myosin phosphatase–Rho interacting protein (MRIP) was upregulated, and myosin phosphatase target subunit 1 (MYPT1) was downregulated in PFCs. Fibrogenic changes were significantly upregulated in both PFCs and UV-NCFCs compared to NCFCs. We found that the activation of the S1P receptor–Rho cascade was observed in pterygium tissue. Additionally, in vitro examination showed S1P–rho activation and fibrogenic changes in PFCs and UV-NCFCs. S1P elevation and the resulting upregulation of the downstream Rho signaling pathway may be important in pterygium formation; this pathway offers a potential therapeutic target for suppressing pterygium generation.
Hydrogel-Mediated DOX⋅HCl/PTX Delivery System for Breast Cancer Therapy
Authors: Hyun Hoon , Yoo Young , Kim So , Ko Hyun , Chun Heung , Yang Dae
Published: September 2019

We used a hydrogel-mediated dual drug delivery approach, based on an injectable glycol chitosan (GC) hydrogel, doxorubicin hydrochloride (DOX⋅HCl), and a complex of beta-cyclodextrin (β-CD) and paclitaxel (PTX) (GDCP) for breast cancer therapy in vitro and in vivo. The hydrogel was swollen over 3 days and remained so thereafter. After an initial burst period of 7 hours, the two drugs were released in a sustained manner for 7 days. The in vitro cell viability test showed that GDCP had a better anticancer effect than well plate and DOX⋅HCl/PTX (DP). In addition, the in vivo tests, which evaluated the anticancer effect, systemic toxicity, and histology, proved the feasibility of GDCP as a clinical therapy for breast cancer.