bluuebunny/crossref_metadata_2025 huggingface dataset:
Hormone therapy improves conservation breeding outcomes in the critically endangered Baw Baw frog, Philoria frosti
Authors: Gilbert Deon J. , Goodall Damian , Byrne Phillip G. , Silla Aimee J.
Published: October 2024

Conservation breeding programs (CBPs) are often the lifeline between extinction and survival for many imperilled amphibian species. With the goal of recovering wild populations, CBP success is reliant on their ability to successfully manage ex situ populations over time, breed viable offspring, and maintain genetic diversity and adaptive potential. Reproductive technologies have emerged as an important tool in the conservation toolkit to allow managers to improve reproductive output and genetic management, and their use in amphibian conservation is expanding. To date, studies investigating the efficacy of hormone therapies in amphibians typically only report spawning and fertility rates and do not monitor offspring to later stages of development. For the first time, here we assess the effect of hormone therapies on captive breeding outcomes beyond oviposition, to the point of metamorphosis, in the critically endangered Baw Baw frog, Philoria frosti. To determine the effect of hormone therapy on spawning success and offspring viability, male-female pairs were administered either 0 µg/g gonadotropin-releasing hormone agonist (GnRHa), 0.5 µg/g GnRHa, or 0.5 µg/g GnRHa + 10 µg/g metoclopramide (MET) (n = 12 pairs/treatment), and the number of pairs ovipositing, time to oviposition, clutch size, metamorph mass, and the proportion and number (mean and total) of offspring to metamorphosis were quantified. Overall, the percentage of pairs that oviposited was high across all treatment groups (92-100%). The percentage of fertile clutches was highest in the GnRHa group (92%) and lowest in the GnRHa + MET group (82%), though differences were not statistically significant. Both hormone treatment groups took significantly less time to oviposit than the control pairs. Notably, the proportion of eggs developing to metamorphosis was significantly higher in the GnRHa group, resulting in 74% (total eggs=539) metamorphosing compared to approximately 50% in the control and GnRHa+MET treatments (total eggs= 273 and 264, respectively). Interestingly, weight at metamorphosis was statistically similar across all groups, and results are consistent with previous studies in this species that show a narrow range in size at metamorphosis. The continued application of GnRHa is recommended to improve conservation outcomes for the critically endangered Baw Baw frog. The outcomes of this research advance our understanding of the impact of hormone therapies on reproductive outcomes and will inform amphibian conservation breeding programs globally.
The impact of anesthesia clerkship training on procedural timing: a focus on medical students in anesthesiology
Authors: Chatmongkolchart Sunisa , Saetang Mantana , Kunapaisal Thitikan , Yongsata Dararat , Saelim Kantaros
Published: October 2024

Introduction

The impact of medical student involvement in anesthetic procedures on operating room (OR) efficiency and patient outcomes remains understudied, despite its importance in medical education. This study aimed to quantify the effects of fifth-year medical students’ participation in anesthetic procedures on key time metrics, perceived difficulty, procedural success, and postoperative complications and to compare these effects with those of anesthetic residents and student nurse anesthetists.

Methods

This prospective observational study was conducted between April and July 2022 in the OR of a tertiary university hospital in southern Thailand. We analyzed 111 elective obstetric-gynecologic and general surgery procedures under subarachnoid block or general anesthesia, equally distributed among three groups: fifth-year medical students, anesthetic residents, and student nurse anesthetists. Key time intervals, perceived difficulty, number of attempts, success rates, and postoperative complications were recorded and compared across groups.

Results

Fifth-year medical students exhibited significantly prolonged anesthesia release time, anesthesia-controlled time, anesthetic preparation time, and anesthetic procedure time compared to anesthetic residents and student nurse anesthetists (p < 0.05). Medical students perceived case inductions as more challenging than the other groups did (p < 0.001), but procedural success rates were comparable across groups. Immediate postoperative airway complications were more frequent among medical students than anesthetic residents, but no significant differences were found in intraoperative or 24-h postoperative complications.

Discussion

While the involvement of medical students in anesthetic procedures extends specific time metrics, it does not significantly impact overall case duration or major patient outcomes. These findings support the continued integration of medical student training in the OR, with appropriate supervision to manage the slightly increased risk of immediate postoperative complications. Further research is needed to optimize the balance between educational opportunities and OR efficiency.

Clinical trial registration

https://www.thaiclinicaltrials.org/show/TCTR20220418006, identifier TCTR20220418006.
Use of connected injection device has a positive effect on catch-up growth in patients with growth disorders treated with growth hormone therapy
Authors: de Arriba Antonio , van Dommelen Paula , Savage Martin O.
Published: October 2024

Introduction

Human growth hormone (hGH) therapy in children can be administered by subcutaneous injection using either a manual non-connected device, which is a portable injection pen loaded with a pre-filled cartridge, or an electronic connected device. The electronic device is connected to a platform where adherence data is recorded and available for health care professionals (HCPs) and patient support programs. Real-world data used in the clinic, includes regular monitoring of adherence data which are shared with families during patients’ visits and aim to determine the root causes of poor adherence. This study aimed to identify whether there are differences in growth during the first four years of treatment depending on the device, i.e. non-connected versus connected devices.

Methods

This retrospective study reports treatment of either GH deficiency or short stature secondary to birth size small for gestational age (SGA) in 174 pediatric patients attending Miguel Servet Hospital, Zaragoza, Spain. hGH treatment was administered with manual non-connected devices in 87 patients and 87 patients used connected devices. Height was followed for 4 years after start of hGH therapy.

Results

In total, 57% of subjects had GHD and 43% were SGA. Height standard deviation score (HSDS) at treatment start was higher (p<0.001) in the non-connected device group compared to the connected device group. Change of HSDS in the connected device group was significantly higher in the second (+0.13), third (+0.20) and fourth (+0.23) year of treatment compared to the non-connected group after adjustment for age and HSDS at treatment start, sex, indication, dose and Tanner stages during treatment, and timing of measurements.

Discussion

These results support the use of the connected device for hGH treatment of pediatric growth disorders.
Economic operation strategy of an electricity–heat coupling system considering complementary low-carbon characteristics of source-load
Authors: Tang Jianlin , Xiao Yanhong , Qian Bin , Hu Houpeng , Zhou Mi , Ou Jiaxiang , Wang Ji , Chen Ruize
Published: October 2024

The operation effects of a source-side carbon capture power plant (CCPP) and power-to-gas (P2G) equipment do not match. The response range of the load-side traditional demand response strategy is small, and the adjustment period is limited, which leads to the problem that the complementary potential of low-carbon characteristics on both sides of the source and load is not fully utilized. This article proposes an electricity–heat coupling system scheduling strategy considering the complementary low-carbon characteristics of “source-load.” First, the low-carbon operation characteristics of CCPP and P2G with integrated, flexible operations are analyzed, and a source-side CCPP-P2G comprehensive flexible operation mode is proposed. Second, based on the characteristics of flexible adjustment and mutual substitution of electricity and heat load, a load-side comprehensive demand response method is proposed. Finally, the complementary mechanism of low-carbon characteristics on both sides of the source and load is analyzed, and a low-carbon economic dispatch model of the electricity–heat coupling system is constructed to realize the source–load collaborative low-carbon operations. The simulation analysis verifies that the proposed strategy can give full play to the energy time shift advantages of the source-side CCPP and P2G and improve the economic and environmental benefits of the system operations.
Dynamic roles of neutrophil extracellular traps in cancer cell adhesion and activation of Notch 1-mediated epithelial-to-mesenchymal transition in EGFR-driven lung cancer cells
Authors: Dimitrov Jelena , Maddalena Maurizio , Terlizzi Cristina , Altobelli Giovanna G. , Pellegrino Sara , Mehmood Tayyaba , De Rosa Viviana , Iommelli Francesca , Del Vecchio Silvana
Published: October 2024

Introduction

Neutrophil extracellular traps (NETs) are complex structures released by activated neutrophils that may modulate different steps of the metastatic cascade. The aim of our study was to investigate how NETs can modulate the adhesion properties of cancer cells and whether cell exposure to NETs can activate the epithelial-to-mesenchymal transition (EMT) program thus enhancing the migratory and invasive properties of tumor cells.

Materials and methods

Different cancer cell lines were subjected to a solid-phase adhesion assay using NET-coated plates with or without the addition of antibodies against α5β1 or CCDC25 receptor. After 1-4 h of incubation, adherent cells were expressed as the percentage of total cell number. To test EMT occurrence, cells were treated with NETs for up to 48 h and then the levels of E-cadherin, vimentin, Snail, Slug, Zeb 1 and Twist 1 along with levels of Notch 1 and cleaved Notch 1 were determined by western blotting. Untreated and NET-treated cells were subjected to migration assays using 24-multiwell plates with transwell and FBS as chemoattractant.

Results

Cancer cell adhesion to NET-coated plates varied between 30% and 92.7% and was significantly higher than that obtained in uncoated plates. The addition of antibodies against α5β1 or CCDC25 caused a strong reduction of cell adhesion to NETs. The prolonged exposure of EGFR-driven cancer cell lines to NETs caused the activation of the EMT program through the upregulation and cleavage of Notch 1 and was confirmed by the enhanced expression of EMT markers. The consequent loss of the epithelial phenotype induced a strong reduction of the expression of the oncogene driver. Cell migration was significantly enhanced in NET-treated cells as compared to untreated cells.

Discussion

Our findings reveal the dynamic role of NETs that may provide a DNA and fibronectin rich environment for binding of many cancer cells at distant sites where the prolonged exposure to NETs triggers the EMT through the activation of Notch 1 signaling pathway with the subsequent enhancement of migratory and invasive properties of cancer cells. Furthermore, our findings provide an example of how an immune/inflammatory microenvironment may directly modulate the sensitivity of cancer cells to oncogene targeted agents.
A hybrid machine learning model combining association rule mining and classification algorithms to predict differentiated thyroid cancer recurrence
Authors: Firat Atay Feyza , Yagin Fatma Hilal , Colak Cemil , Elkiran Emin Tamer , Mansuri Nasrin , Ahmad Fuzail , Ardigò Luca Paolo
Published: October 2024

Background

Differentiated thyroid cancer (DTC) is the most prevalent endocrine malignancy with a recurrence rate of about 20%, necessitating better predictive methods for patient management. This study aims to create a relational classification model to predict DTC recurrence by integrating clinical, pathological, and follow-up data.

Methods

The balanced dataset comprises 550 DTC samples collected over 15 years, featuring 13 clinicopathological variables. To address the class imbalance in recurrence status, the Synthetic Minority Over-sampling Technique for Nominal and Continuous (SMOTE-NC) was utilized. A hybrid model combining classification algorithms with association rule mining was developed. Two relational classification approaches, regularized class association rules (RCAR) and classification based on association rules (CBAR), were implemented. Binomial logistic regression analyzed independent predictors of recurrence. Model performance was assessed through accuracy, sensitivity, specificity, positive predictive value, negative predictive value, and F1 score.

Results

The RCAR model demonstrated superior performance over the CBAR model, achieving accuracy, sensitivity, and F1 score of 96.7%, 93.1%, and 96.7%, respectively. Association rules highlighted that papillary pathology with an incomplete response strongly predicted recurrence. The combination of incomplete response and lymphadenopathy was also a significant predictor. Conversely, the absence of adenopathy and complete response to treatment were linked to freedom from recurrence. Incomplete structural response was identified as a critical predictor of recurrence risk, even with other low-recurrence conditions.

Conclusion

This study introduces a robust and interpretable predictive model that enhances personalized medicine in thyroid cancer care. The model effectively identifies high-risk individuals, allowing for tailored follow-up strategies that could improve patient outcomes and optimize resource allocation in DTC management.
Study on effect of plant growth promoting rhizobacteria on sorghum (Sorghum bicolor L.) under gnotobiotic conditions
Authors: Chiranjeevi M. , Goudar Geeta D. , PU Krishnaraj , Yalavarthi Nagaraju
Published: October 2024

The rhizosphere is enriched with diverse microflora, allowing for delving prospective microorganisms to enhance crop growth and yield for varied soil conditions. Demand for millet growth-promoting microorganisms is a contemporary need for dryland agriculture. Therefore, a detailed survey was conducted in northern Karnataka, India, to identify the millet growing areas, particularly sorghum. The rhizobacteria from the sorghum (Sorghum bicolor L.) were assessed for promoting seed germination using the paper towel method and classified based on their efficiency. The elite isolates were positive for indole-3-acetic acid (IAA), gibberellic acid (GA), phosphate, zinc oxide solubilization, and hydrogen cyanide (HCN) production. The test isolates were antagonistic to Macrophomina phaseolina and Fusarium sp. and inhibited completely. Further evaluation of the cultures on sorghum growth-promoting attributes under pot culture conditions showed that the plants inoculated with PG-152 (Bacillus subtilis) recorded the highest plant height, chlorophyll content, root dry weight, shoot dry weight, and total dry weight under ideal conditions of fertilization. Two isolates, namely, PG-152 and PG-197, performing superior under pot culture conditions, were identified as Bacillus subtilis and PG-197 as Enterobacter sp., respectively, using 16S rDNA analysis. The sequences were allowed to screen open reading frames (ORF) and found several ORFs in Enterobacter cloacae and Bacillus subtilis, respectively. This study found that the rhizosphere is vital for identifying prospective isolates for biocontrol and plant growth-improving microorganisms.
Exploring genetic determinants of antimicrobial resistance in Brucella melitensis strains of human and animal origin from India
Authors: Ayoub Haris , Kumar M. Suman , Mehta Rishabh , Thomas Prasad , Dubey Muskan , Dhanze Himani , Ajantha Ganavalli S. , Bhilegaonkar K. N. , Salih Harith M. , Cull Charley A. , Veeranna Ravindra P. , Amachawadi Raghavendra G.
Published: October 2024

Introduction

Antimicrobial resistance (AMR) in Brucella melitensis, the causative agent of brucellosis, is of growing concern, particularly in low and middle-income countries. This study aimed to explore the genetic basis of AMR in B. melitensis strains from India.

Methods

Twenty-four isolates from humans and animals were subjected to antimicrobial susceptibility testing and whole-genome sequencing.

Results

Resistance to doxycycline (20.80%), ciprofloxacin (16.67%), cotrimoxazole (4.17%), and rifampicin (16.67%) was observed. Genome analysis revealed efflux-related genes like mprF, bepG, bepF, bepC, bepE, and bepD across all isolates, however, classical AMR genes were not detected. Mutations in key AMR-associated genes such as rpoB, gyrA, and folP were identified, intriguingly present in both resistant and susceptible isolates, suggesting a complex genotype–phenotype relationship in AMR among Brucella spp. Additionally, mutations in efflux genes were noted in resistant and some susceptible isolates, indicating their potential role in resistance mechanisms. However, mutations in AMR-associated genes did not consistently align with phenotypic resistance, suggesting a multifactorial basis for resistance.

Discussion

The study underscores the complexity of AMR in B. melitensis and advocates for a holistic multi-omics approach to fully understand resistance mechanisms. These findings offer valuable insights into genetic markers associated with AMR, guiding future research and treatment strategies.
Comparison of autism spectrum disorder subtypes based on functional and structural factors
Authors: Wang Shan , Sun Zhe , Martinez-Tejada Laura Alejandra , Yoshimura Natsue
Published: October 2024

Autism spectrum disorder (ASD) is a series of neurodevelopmental disorders that may affect a patient’s social, behavioral, and communication abilities. As a typical mental illness, ASD is not a single disorder. ASD is often divided into subtypes, such as autism, Asperger’s, and pervasive developmental disorder-not otherwise specified (PDD-NOS). Studying the differences among brain networks of the subtypes has great significance for the diagnosis and treatment of ASD. To date, many studies have analyzed the brain activity of ASD as a single mental disorder, whereas few have focused on its subtypes. To address this problem, we explored whether indices derived from functional and structural magnetic resonance imaging (MRI) data exhibited significant dissimilarities between subtypes. Utilizing a brain pattern feature extraction method from fMRI based on tensor decomposition, amplitude of low-frequency fluctuation and its fractional values of fMRI, and gray matter volume derived from MRI, impairments of function in the subcortical network and default mode network of autism were found to lead to major differences from the other two subtypes. Our results provide a systematic comparison of the three common ASD subtypes, which may provide evidence for the discrimination between ASD subtypes.
The value of chromosome instability detected by low−pass whole−genome sequencing in preoperative prediction of sentinel lymph node metastasis in breast cancer
Authors: Zheng Jian , Xu Fen , Li Guangying , Lin Moubin , Hao Hua
Published: October 2024

Background

Breast cancer is a malignancy characterized by chromosomal instability (CIN). This study aimed to examine the potential diagnostic value of chromosomal instability, detected by low-pass whole-genome sequencing (LPWGS), in the preoperative evaluation of sentinel lymph node metastasis (SLNM) in breast cancer.

Methods

A retrospective investigation of clinical records from 29 patients with breast cancer revealed two distinct groups based on sentinel lymph node biopsy (SLNB) results: the SLN metastasis group (24 cases) and the SLN non-metastasis group (five cases). CIN and CIN scores were evaluated using LPWGS. An analysis of univariate data and binary logistic regression was employed to identify factors influencing SLNM, and a curve with receiver operating characteristics (ROC) was constructed to assess the diagnostic utility of CIN in predicting SLNM.

Results

A significant association between the SLNM and CIN high groups was observed in breast cancer (P=0.011). The CIN score in the metastasis group (17,665.055 ± 8,630.691) was higher than that in the non-metastasis group (9,247.973 ± 3,692.873), demonstrating a significant difference (P=0.044). Univariate binary logistic regression analysis indicated that CIN was a significant predictor for SLNM (odds ratio: 4.036, 95% CI: 1.015–16.047, P=0.048). The AUC of CIN for preoperative diagnosis of SLNM was 0.808 (95%CI: 0.635–0.982, P=0.033), with a sensitivity value of 67.0% and specificity of 100.0% at a threshold of 13,563.

Conclusion

Detecting CIN through LPWGS demonstrates diagnostic potential in predicting SLNM in patients with breast cancer before surgery. This approach offers a novel method for assessing axillary lymph node status in clinical practice.
Case report: Imaging of adrenal adenomatoid tumors: reports of two cases and review of literature
Authors: Wu Yuanyuan , Hu Dongliang , Cui Manman , Liu Yan , Zhou Xiuzhi , Zhai Duchang , Fan Guohua , Cai Wu
Published: October 2024

Adenomatoid tumors (ATs) are uncommon, benign tumors of mesothelial origin, most frequently found in the genital tracts of both sexes. Extragenital localization sites, such as adrenal glands, are extremely rare. Since patients with adrenal ATs have no obvious clinical symptoms, imaging examination has become important evidence for diagnosis. Although previous literature noted that the imaging findings of adrenal ATs were nonspecific, no relevant studies have confirmed this. We herein present two novel cases of adrenal ATs, confirmed by immunohistochemistry, and that were initially misdiagnosed as other, more common adrenal tumors based on clinical findings and preoperative imaging. Including our cases, we collected a total of 33 previously reported adrenal ATs and extracted all available imaging information from them, aiming to find some radiological characteristics of this rare tumor. Through the review, we identified some nonspecific imaging features of adrenal ATs; however, the final diagnosis still depends on pathology and immunohistochemistry results.
Assessment of synthetic MRI to distinguish Warthin’s tumor from pleomorphic adenoma in the parotid gland: comparison of two methods of positioning the region of interest for synthetic relaxometry measurement
Authors: Sun Jiabin , Kuai Xinping , Huang Dawei , Ji Xinghua , Jia Chuanhai , Wang Shengyu
Published: October 2024

Purpose

To assess the diagnostic potential of the synthetic MRI (SyMRI) for differentiating Warthin’s tumors (WT) from pleomorphic adenomas (PA).

Materials and methods

Forty-nine individuals with parotid gland tumors (PA, n = 23; WT, n = 26) were recruited. Using two distinct regions of interest (ROI), SyMRI quantitative parameters of lesions were calculated, including mean and standard deviation (T1, T2, PD, T1sd, T2sd, and PDsd). Meanwhile, T1ratio, T2ratio, and PDratio (lesion/masseter muscle) were calculated based on the mean SyMRI quantitative parameters of masseter muscle (T1, T2, PD). Using the independent samples t test, we compared PA and WT parameters, while comparing the areas under the curve (AUC) using the DeLong’s test. A multi-parameter SyMRI model was constructed using logistic regression analysis.

Results

In PA, the T1, T1sd, T2, PD, T1ratio, T2ratio, and PDratio derived from full and partial lesion ROIs were significantly higher than in WT. According to the receiver operating curve analysis, the AUC of the quantitative parameters derived from full-lesion and partial-lesion ROIs ranged from 0.722 to 0.983 for differentiating PA from WT. T1 values derived from partial-lesion ROI delineation demonstrated the best diagnostic performance among all single parameters, achieving an AUC of 0.983. Using 1322 ms as a cutoff value, the sensitivity, specificity, and accuracy were 88.46%, 100% and 93.88%, respectively.

Conclusion

The SyMRI-derived quantitative parameters demonstrated excellent performance for discriminating PA from WT in the parotid gland.
Stress and career aspirations: a longitudinal study with medical students
Authors: Picker-Roesch Clara , Lang Jessica
Published: October 2024

Background

The psychological well-being of medical students has often been studied in relation to various factors and typically exhibits poorer scores than comparable student groups. This diminished well-being can have far-reaching implications, like impact on career or academic engagement. While there are some studies on specialty choice or academic performance few studies have examined the effects of stress at the beginning of medical school on career ambitions and the associated interest in research. Given the anticipated future shortage of healthcare professionals and the goal of gender equality in higher positions, it is crucial to examine this relationship as early as medical education. The question arises whether medical education and the demands of studying influence the career ambitions of different student groups.

Method

As part of a longitudinal survey, students from a medical cohort are surveyed over three consecutive years. A total of 297 students participated in the surveys, of which 66.4% were women. Stress experiences during medical school and career aspirations are assessed and analyzed using Generalized Estimating Equation (GEE) modeling.

Results

The results indicate that early academic stress is a negative predictor of the career ambitions of medical students (B = 1.637; p = 0.009). Also, female students report lower career goals than male students (B = 1.168; p = 0.018). In addition, gender moderates the impact of stress on career ambitions (p = 0.046). Finally, stress levels are highest when starting the medical program (p = 0.022) and decrease over time.

Discussion

Stress during the initial medical training impedes students to pursue higher career ambitions. Future research as well as medical programs need to elaborate more on and consider structural stress prevention interventions in order to promote well-being and strengthen also scientific career goals at an early stage, specifically for female students.
The mediating role of school effectiveness in the relationship between transformational leadership and workplace exclusion
Authors: Kandemir Ayhan
Published: October 2024

The purpose of this study is to reveal the mediating role of school effectiveness in the relationship between transformational leadership and workplace exclusion. The sample of the study consisted of 410 teachers working in primary, secondary and high schools in Bolu/Türkiye province center in the 2023–2024 academic year. Correlational design was used in the study and path analysis was used to reveal the predictive relationships between variables. As a result of the study, it was seen that the hypotheses put forward in line with the model put forward were confirmed. In this context, it was concluded that transformational leadership has a significant positive effect on school effectiveness (Hypothesis 1), school effectiveness has a significant negative effect on workplace exclusion (Hypothesis 2), transformational leadership has a significant negative effect on workplace exclusion (Hypothesis 3) and finally school effectiveness has a mediating role in the effect of transformational leadership on workplace exclusion (Hypothesis 4). In the context of the results, suggestions were made such as providing in-service courses for school administrators on transformational leadership and school effectiveness, preventing exclusion at work by giving teachers the opportunity to develop themselves and participate in the decisions taken.
The potential of muscarinic M1 and M4 receptor activators for the treatment of cognitive impairment associated with schizophrenia
Authors: Yohn Samantha E. , Harvey Phillip D. , Brannan Stephen K. , Horan William P.
Published: October 2024

Cognitive impairment is a core symptom of schizophrenia and a major determinant of poor long-term functional outcomes. Despite considerable efforts, we do not yet have any approved pharmacological treatments for cognitive impairment associated with schizophrenia (CIAS). A combination of advances in pre-clinical research and recent clinical trial findings have led to a resurgence of interest in the cognition-enhancing potential of novel muscarinic acetylcholine receptor (mAChR) agonists in schizophrenia. This article provides an overview of the scientific rationale for targeting M1 and M4 mAChRs. We describe the evolution of neuroscience research on these receptors since early drug discovery efforts focused on the mAChR agonist xanomeline. This work has revealed that M1 and M4 mAChRs are highly expressed in brain regions that are implicated in cognition. The functional significance of M1 and M4 mAChRs has been extensively characterized in animal models via use of selective receptor subtype compounds through neuronal and non-neuronal mechanisms. Recent clinical trials of a dual M1/M4 mAChR agonist show promising, replicable evidence of potential pro-cognitive effects in schizophrenia, with several other mAChR agonists in clinical development.
Religion, spirituality, and pediatric mental health: a scoping review of research on religion and spirituality in the Journal of the American Academy of Child and Adolescent Psychiatry from 2000 to 2023
Authors: Elzamzamy Khalid , Naveed Sadiq , Dell Mary Lynn
Published: October 2024

Introduction

Religion and spirituality (R/S) serve as sources of meaning-making and coping for many individuals and families. While research on the relationship between R/S and mental health has been ongoing, their role in pediatric mental health is poorly understood. The objective of this study is to assess research trends and predominant themes of R/S in child and adolescent psychiatric research in articles published in the Journal of the American Association of Child and Adolescent Psychiatry (JAACAP). This provides a rough measure of the relative importance of these topics to academic psychiatrists and researchers in the field and identifies gaps for future research.

Methods

All research and review articles published in JAACAP between 2000-2023 with a focus on R/S themes were retrieved, screened, and appraised for content and extent of focus on R/S (major, minor, incidental). Included articles were assessed for R/S variables and predominant themes.

Results

Thirty-two (32) research articles published between 2000-2023 contained sufficient R/S content for inclusion in our study. Only 4 articles had R/S as their major focus. Our analysis suggested a decline in publications with R/S content over the last 24 years. The R/S variables and measurement tools were heterogenous, with religious attendance and religious affiliation being the most frequently measured variables. The predominant themes include the relationships between R/S and psychopathology, suicide, utilization of services, conceptualization of illness, trauma, identity, and coping.

Discussion

Despite the significance attributed to R/S by many youths and families and the increasing academic interest in the intersection between R/S and health, a significant gap exists in our understanding of R/S vis-à-vis child and adolescent mental health. This gap may be further compounded by the limited attention offered to R/S factors and variables in academic psychiatric activities. JAACAP, a global leading academic platform, may advance this discourse by inviting and encouraging publications addressing R/S variables. This may inform diagnostic, preventive, and interventive clinical work with children and their families.
Influencing Canadian young adults to receive additional COVID-19 vaccination shots: the efficacy of brief video interventions focusing on altruism and individualism
Authors: Batra Rachita , Tatar Ovidiu , Zhu Patricia , Perez Samara , Haward Ben , Zimet Gregory , Rosberger Zeev
Published: October 2024

Younger adults, aged 18–39 years, exhibit low COVID-19 additional vaccine (i.e., vaccination beyond the original 2-dose series) uptake recommended in Canada. No study has examined how altruistic and individualistic messaging can influence COVID-19 additional dose intentions. The present study aimed to estimate the efficacy of altruism and individualism-based videos on vaccine intentions and to explore the multivariable associations between vaccine related individual psychosocial factors and intention to receive the COVID-19 vaccine. Using a web-based survey in a three-arm, pre-post randomized control trial design, we recruited Canadians aged 18–39 years in both English and French. Participants were randomly allocated in a 1:1:1 ratio to receive the active control (COVID-19 general information), control + altruism or control + altruism + individualism. The video interventions were developed with a media company, based on results of a focus group study conducted previously. The measurement of COVID-19 additional dosage intentions before and after completing the interventions was informed by the multistage Precaution Adoption Process Model. The McNemar Chi-square was used to evaluate within-group changes, and the Pearson Chi-square test was used to evaluate between-group changes post-intervention. The measurement of various psychosocial factors was informed by use of validated scale and self-report questions. We employed a generalized Structural Equation Model to evaluate the associations between COVID-19 vaccine intentions and the psychosocial factors. Analyses were performed on 3,431 participants (control: n = 1,149, control + altruism: n = 1,142, control + altruism + individualism: n = 1,140). Within-group results showed that participants transitioned significantly in all three groups in the direction of higher intentions for receiving additional COVID-19 vaccine doses. The between-group differences in post intervention vaccine intentions were not significant. We found that psychosocial factors that include, collectivism, intellectual humility, intolerance to uncertainty, religiosity, identifying as gender diverse, and being indigenous were associated with higher vaccine intentions, whereas pandemic fatigue was associated with lower vaccine intentions. Our study highlighted that a short video that includes altruism and individualism messaging or general COVID-19 information can increase intentions to vaccine among young adults. Furthermore, we gained a comprehensive understanding of various psychosocial factors that influence ongoing COVID-19 vaccination. Our findings can be used to influence public health messaging around COVID-19 vaccination.
Deer antler stem cells immortalization by modulation of hTERT and the small extracellular vesicles characters
Authors: Chen Ze , Meng Deshuang , Pang Xin , Guo Jia , Li Tiejun , Song Jun , Peng Yinghua
Published: October 2024

Background

Deer antler stem cells (AnSCs) exhibit properties of both embryonic and mesenchymal stem cells, with superior self-renewal and proliferation, which drive rapid antler growth and regeneration. AnSCs and their derived small extracellular vesicles (sEVs) hold promising potential for applications in regeneration medicine. Due to the restricted proliferative capacity inherent in primary cells, the production capacity of AnSCs and their sEVs are limited. Human telomerase reverse transcriptase (hTERT) is the most important telomerase subunit, hTERT gene insertion has been successfully employed in generating immortalized cell lines.

Results

In this study, we successfully established immortalized AnSCs by transducing the hTERT gene using lentivirus. Compared to primary AnSCs, hTERT-AnSCs demonstrated extended passage potential and accelerated proliferation rates while maintaining the mesenchymal stem cell surface markers CD44 and CD90. Additionally, hTERT-AnSCs retained the capacity for osteogenic, adipogenic, and chondrogenic differentiation. sEVs derived from hTERT-AnSCs exhibited a particle size distribution similar to that of AnSCs, both displaying a cup-shaped morphology and expressing CD81, ALIX, and TSG101, while notably lacking GM130 expression.

Conclusion

We successfully isolated primary stem cells from deer antler and established the immortalized hTERT-AnSCs. Remarkably, this cell line maintains its stem cell characteristics even after 40 passages. The sEVs derived from these cells exhibit identical morphological and structural features to those of primary AnSCs. This research provides essential technical support for the application of AnSCs and their sEVs in regenerative medicine.
No Interaction, No Problem? An Investigation of Organizational Issues in the University–Industry–Government Triad in a Transition Economy
Authors: Landoni Matteo , Muradzada Nijat
Published: October 2024

Transition economies, on the one hand, grapple with a communist legacy; on the other hand, they seek the optimal institutionalization for knowledge generation, dissemination, and commercialization to compete globally. However, the incumbent knowledge of certain aspects of their innovation systems remains very limited. In particular, intra-organizational cultural relics of the past and their inter-organizational and, consequently, systemic implications require research. This study examines how interaction barriers among universities, industry, and government, stemming from intra-organizational cultures, impact structural change in the innovation system of Azerbaijan. Utilizing the TH model, interviews with 59 participants revealed that a “Statist” TH model in Azerbaijan hinders organic cultural development within organizations, leading to interaction issues among TH actors. Moreover, problems in inter-organizational communication pave the way for a systemic failure that necessitates government intervention, strengthening the “Statist” TH model. The findings increase the context sensitivity of the TH framework by exploring an understudied context and provide valuable insights relevant to other transition economies facing similar institutional legacies.
The Role of Business Angels in the Early-Stage Financing of Startups: A Systematic Literature Review
Authors: Lange Jürgen , Rezepa Stefan , Zatrochová Monika
Published: October 2024

Funding is an essential factor for the viability and growth of startups. As a result, business angels play a crucial role in providing financial support to these business companies, particularly those that are innovative and have significant potential for growth. This study sought to determine the role business angels play in the early-stage financing of startups. Specifically, the study looked at the value-added services provided by business angels, business angel funding impact on startup survival rates, the effectiveness of business angel networks’ impact on facilitating startup funding, and business angels’ contribution to the development of entrepreneurial ecosystems beyond financial investment for startups. This study adopted a systematic literature review methodology, employing key theoretical methods such as analysis, synthesis, comparison, and induction to assess the role business angels play in the early-stage financing of startups. The findings show that business angels’ expertise, networks, and mentorship emerge as critical value-added startup services. Similarly, it was found that business angel funding positively influences startup survival; however, other factors also influence this impact. Moreover, the results show that business angel networks play a significant role in facilitating startup funding. Furthermore, beyond financial investment for startups, it was found that business angels contribute significantly to the development of entrepreneurial ecosystems, including prioritizing the contributions of ecosystem builders in startup screening, access to mentoring, and entrepreneurial education. The study concluded that business angels play a positive role in the early-stage financing of startups.
Method for Underground Motion Using Vibration-Induced Ground Resistance Changes for Planetary Exploration
Authors: Watanabe Tomohiro , Kobayashi Koya , Hiramoto Kazuhiko , Iizuka Kojiro
Published: October 2024

Exploration rovers have difficulty moving underground because the drag force from the ground restricts their movement; this hinders underground exploration. This study aimed to address this challenge. We posit a hypothesis that the rover can move underground by imparting vibration to the ground and changing the drag force. To validate this hypothesis, a testbed that moves underground was developed, and the drag force when imparting vibration was investigated. The results revealed that the drag force while imparting vibration is smaller than that after imparting vibration, and we accordingly devised the operation for moving underground. The proposed operation causes bias of the drag force by imparting vibration to make the testbed move in the direction of the small drag force. The effectiveness of the proposed method was assessed through an experiment wherein the testbed was set to move underground. The experimental results demonstrate the superiority of the proposed method, as the movement distance achieved with vibration is considerably greater than that without vibration. The findings validate the hypothesis that using vibration for underground motion is effective in improving mobility and provides valuable insights into the design of robots for underground motion.
Resource Allocation Approach of Avionics System in SPO Mode Based on Proximal Policy Optimization
Authors: Dong Lei , Liu Jiachen , Sun Zijing , Chen Xi , Wang Peng
Published: October 2024

Single-Pilot Operations (SPO) mode is set to reshape the decision-making process between human-machine and air-ground operations. However, the limited on-board computing resources impose greater demands on the organization of performance parameters and the optimization of process efficiency in SPO mode. To address this challenge, this paper first investigates the flexible requirements of avionics systems arising from changes in SPO operational scenarios, then analyzes the architecture of Reconfigurable Integrated Modular Avionics (RIMA) and its resource allocation framework in the context of scarcity and configurability. A “mission-function-resource” mapping relationship is established between the reconfiguration service elements of SPO mode and avionics resources. Subsequently, the Proximal Policy Optimization (PPO) algorithm is introduced to simulate the resource allocation process of IMA reconfiguration in SPO mode. The objective optimization process is transformed into a sequential decision-making problem by considering constraints and optimization criteria such as load, latency, and power consumption within the feasible domain of avionics system resources. Finally, the resource allocation scheme for avionics system reconfiguration is determined by controlling the probability of action selection during the interaction between the agent and the environment. The experimental results show that the resource allocation scheme based on the PPO algorithm can effectively reduce power consumption and latency, and the DRL model has strong anti-interference and generalization. This enables avionics resources to respond dynamically to the capabilities required in SPO mode and enhances their ability to support the aircraft mission at all stages.
Vegetables Treated before Drying with Natural Antioxidants plus UV-C Improve Colour and Bioactive Compounds
Authors: Ramírez-Fajardo Antonio-Fer , Martín-Vizcaíno Cristina , Rodríguez-García Ignacio , Guil-Guerrero José Luis
Published: October 2024

The quality of minimally processed fruits and vegetables is essential for consumers’ health and effective marketing. This study investigates the effects of UV-C irradiation, applied alone and combined with various natural antioxidants, on the preservation of bioactive compounds and the colour of dehydrated fruits and vegetables. To achieve this, red peppers, yellow peppers, tomatoes, zucchini, eggplants, and melons were subjected to pre-treatments with natural antioxidants and UV-C before processing using low-temperature airflow (50 °C). The drying kinetics showed typical curves of hygroscopic materials, while the drying time was high due to the low temperature applied. The higher drying rate was found for eggplant, due to its porosity, thus allowing a faster moisture removal. The application of antioxidants and UV-C treatments effectively preserved the colour parameters L*, a*, and b*, while in the case of untreated dried vegetables, a significant worsening of colour parameters was noted. However, most applied pre-treatments had positive effects on bioactive compound losses. The best results were obtained using a combination of UV-C with one antioxidant mix that was composed of vanillin, rosemary, and citrus extracts, and combined with a mixture of olive, onion, garlic, and citric acid extracts, which was highly effective in preserving the colour and bioactive compounds of most dried vegetables.
An Age-Stage, Two-Sex Life Table for Megalurothrips usitatus Feeding on Eight Different Crop Plants
Authors: Wang Huanting , Huang Lifei , Zheng Xialin , Gong Rui , Cao Xuemei , Yang Lang
Published: October 2024

Megalurothrips usitatus (Bagnall) feeds on the young tissues of plants, causing wilting and deformity of leaves as well as damage to flowers and fruits, thereby seriously affecting plant yield. Due to its small size and difficulty of control, the species is one of the most important pests in the legume family. To clarify the occurrence and damage pattern of M. usitatus on field crops, a study was conducted using eight different crops as experimental materials, and an age-stage, two-sex life table was constructed. The population density of M. usitatus was the highest on cowpeas (Vigna unguiculata L. Walp.), followed by green beans (Phaseolus vulgaris L.) and Vigna cylindrica (Vigna unguiculata subsp. cylindrica (L.) Verdc.) in terms of net reproductive rate and the intrinsic rate of increase. In terms of reproductive capacity, cowpeas and V. cylindrica were more suitable for M. usitatus growth and reproduction, followed by green beans. M. usitatus could develop into adults on courgettes (Cucurbita pepo L.), wax gourds (Benincasa hispida Thunb. Cogn.), Momordica charantia (Momordica charantia L.), and soybeans (Glycine max L. Merr.) but had difficulty reproducing on those plants. Cucumber (Cucumis sativus L.) was the least suitable for the survival of M. usitatus. Population parameter analysis showed that the cowpea was the most suitable host plant for M. usitatus, followed by green beans and V. cylindrica. M. usitatus had difficulty reproducing on courgette, wax gourd, M. charantia, and soybeans, and feeding on cucumbers inhibited M. usitatus development and reproduction.
Investigation of the Relationship between Plasma Nesfatin-1 Levels and Neutering in Dogs
Authors: Guvenc-Bayram Gokcen , Semen Zeynep , Yalcin Murat
Published: October 2024

Neutering of dogs, whether male or female, provides various benefits such as contraception, population control, and the prevention of reproductive disorders and undesirable sexual behaviors. However, it is also associated with an increased risk of obesity, which may be directly linked to post-neutering hormonal changes. Our study aims to determine the effects of neutering on plasma levels of nesfatin-1, serotonin, dopamine, TSH, and T4—hormones implicated in obesity and metabolic regulation. Fourteen dogs (seven males and seven females), aged between 1 and 3 years, were included in this study. Male dogs underwent orchiectomy and females underwent ovariohysterectomy. Blood samples were collected before surgery and on days 7 and 14 post-operatively to measure the plasma levels of these hormones using ELISA. The results showed a significant decrease in nesfatin-1, serotonin, and T4 levels, along with a significant increase in TSH levels in both male and female dogs post-neutering. While these hormonal changes are likely part of the body’s adaptive response to neutering, they may represent a potential mechanism that contributes to the long-term tendency toward obesity in neutered dogs.