bluuebunny/crossref_metadata_2025 huggingface dataset:
Brain functional gradient and structure features in adolescent and adult autism spectrum disorders
Authors: Ruan Lili , Chen Guangxiang , Yao Menglin , Li Cheng , Chen Xiu , Luo Hua , Ruan Jianghai , Zheng Zhong , Zhang Dechou , Liang Sicheng , Lü Muhan
Published: July 2024

Understanding how function and structure are organized and their coupling with clinical traits in individuals with autism spectrum disorder (ASD) is a primary goal in network neuroscience research for ASD. Atypical brain functional networks and structures in individuals with ASD have been reported, but whether these associations show heterogeneous hierarchy modeling in adolescents and adults with ASD remains to be clarified. In this study, 176 adolescent and 74 adult participants with ASD without medication or comorbidities and sex, age matched healthy controls (HCs) from 19 research groups from the openly shared Autism Brain Imaging Data Exchange II database were included. To investigate the relationship between the functional gradient, structural changes, and clinical symptoms of brain networks in adolescents and adults with ASD, functional gradient and voxel‐based morphometry (VBM) analyses based on 1000 parcels defined by Schaefer mapped to Yeo's seven‐network atlas were performed. Pearson's correlation was calculated between the gradient scores, gray volume and density, and clinical traits. The subsystem‐level analysis showed that the second gradient scores of the default mode networks and frontoparietal network in patients with ASD were relatively compressed compared to adolescent HCs. Adult patients with ASD showed an overall compression gradient of 1 in the ventral attention networks. In addition, the gray density and volumes of the subnetworks showed no significant differences between the ASD and HC groups at the adolescent stage. However, adults with ASD showed decreased gray density in the limbic network. Moreover, numerous functional gradient parameters, but not VBM parameters, in adolescents with ASD were considerably correlated with clinical traits in contrast to those in adults with ASD. Our findings proved that the atypical changes in adolescent ASD mainly involve the brain functional network, while in adult ASD, the changes are more related to brain structure, including gray density and volume. These changes in functional gradients or structures are markedly correlated with clinical traits in patients with ASD. Our study provides a novel understanding of the pathophysiology of the structure–function hierarchy in ASD.
Electrophysiological correlation of auditory selective spatial attention in the “cocktail party” situation
Authors: Liu Hongxing , Bai Yanru , Zheng Qi , Liu Jihan , Zhu Jianing , Ni Guangjian
Published: July 2024

The auditory system can selectively attend to the target source in complex environments, the phenomenon known as the “cocktail party” effect. However, the spatiotemporal dynamics of electrophysiological activity associated with auditory selective spatial attention (ASSA) remain largely unexplored. In this study, single‐source and multiple‐source paradigms were designed to simulate different auditory environments, and microstate analysis was introduced to reveal the electrophysiological correlates of ASSA. Furthermore, cortical source analysis was employed to reveal the neural activity regions of these microstates. The results showed that five microstates could explain the spatiotemporal dynamics of ASSA, ranging from MS1 to MS5. Notably, MS2 and MS3 showed significantly lower partial properties in multiple‐source situations than in single‐source situations, whereas MS4 had shorter durations and MS5 longer durations in multiple‐source situations than in single‐source situations. MS1 had insignificant differences between the two situations. Cortical source analysis showed that the activation regions of these microstates initially transferred from the right temporal cortex to the temporal–parietal cortex, and subsequently to the dorsofrontal cortex. Moreover, the neural activity of the single‐source situations was greater than that of the multiple‐source situations in MS2 and MS3, correlating with the N1 and P2 components, with the greatest differences observed in the superior temporal gyrus and inferior parietal lobule. These findings suggest that these specific microstates and their associated activation regions may serve as promising substrates for decoding ASSA in complex environments.
Bridges and gatekeepers: Employees' willingness to refer qualified candidates on the autism spectrum
Authors: Lup Daniela , Canonico Esther
Published: July 2024

Recruiting neurodiverse talent has increasingly featured on the organizational diversity agenda, yet recruitment practices geared toward locating and attracting neurodivergent candidates remain understudied. While research and practice have shown that employee referral is one of the channels through which a significant part of new talent is recruited, little is known about employees' willingness to refer qualified social contacts who are neurodivergent. We address this limitation by investigating employees' willingness to refer highly qualified candidates on the autism spectrum and some conditions under which they are more or less likely to refer such candidates. We explore these aspects in a study of working individuals, using a mixed‐method approach. The quantitative analysis shows that disclosure of an autism condition to potential referrers might pose some advantage, in that it increases the likelihood of being referred, but this advantage accrues only to male candidates. Furthermore, cues that hint at the social dimension of the “ideal worker,” commonly used in job recruitment materials, are the strongest deterrent for referrers. The qualitative analysis of the reasons behind decisions to refer sheds light on some mechanisms that might explain these findings.
Selective enzymatic debridement and modified Meek technique in the treatment of extensive burns: Preliminary observations
Authors: Minic Jasminka , Vigato Enrico , Shoham Yaron , Lavagnolo Umberto , Governa Maurizio
Published: July 2024

Background

Selective bromelain‐based enzymatic debridement (BED) has emerged as a valid alternative for the treatment of extensive burns, with Total Body Surface Area (TBSA) > 20%. Autologous skin grafting represents the procedure of choice but the scarcity of donor sites remains the main reconstructive challenge. The modified Meek micro‐grafting technique may represent a valid strategy to optimize the final outcome.

Methods

A single‐cohort retrospective analysis was performed, involving nine burn patients (TBSA > 20%) who underwent both BED and subsequently modified Meek technique. Demographic and clinical data (mechanism of injury, surgical treatment, complications, necessity of re‐grafting, further surgery and esthetic outcome) were collected.

Results

All patients had large burns of mixed and deep dermal thickness (first, second, and third degree). All burns were enzymatically debrided postadmission and covered by the modified Meek technique. Local infection due to poor general conditions was the main complication for all patients. All but two patients survived. The selectiveness of the enzymatic debridement and dermal preservation seemed to improve the quality of scars resulting from micro‐grafting. Evaluations performed at 12 ± 2 months postburn showed superior scar quality compared to areas treated with traditional (sheet/mesh) grafts.

Conclusion

Combined BED and Meek techniques may provide an effective synergic combination for the treatment of extensive burns.
Structural characterization of iron azadiethylthiolate complexes: Insights from NMR, crystallography, and ion mobility‐mass spectroscopy
Authors: Liu Yu‐Chiao , Ho Yi‐Chi , Lee Gene‐Hsiang , Chiang Ming‐Hsi , Tseng Mei‐Chun
Published: July 2024

Fe3(CO)12 reacted with azadiethylthiols afforded [Fe2(μ‐S(CH2)2NR(CH2)2S)(CO)6]2 (R = nPr, 1A; iPr, 2A). Structure analysis revealed that 1A features the anti‐(a,e,a,e) configuration, while its iso‐propyl 2A adopts the syn‐(a,e,a,e) arrangement. NMR measurements at room temperature confirmed this structure, showing distinct signals for 2 NCH2 and 2 SCH2 methylene groups. Acid treatment of 1–2 yielded N‐protonated species (1A–H, 2A–H), maintaining the anti‐(a,e,a,e) configuration. Isolation of 1B–H revealed both the syn‐(a,e,e,a) and anti‐(a,e,e,a) configuration by X‐ray single‐crystallography. Protonation of 1A–H induced downfield shifts in methylene proton signals, with NH hydrogen recorded at 6.96 ppm. Furthermore, 1B–H displays similar NMR behavior, indicating the coexistence of two isomeric molecules of 1B–H, syn‐(a,e,e,a) and anti‐(a,e,e,a), which is consistent with the crystallographic results. Moreover, ion mobility‐mass spectrometry (IM‐MS) is harnessed for the swift identification and differentiation of these isomeric complexes in solution. By leveraging accurate mass measurements and MS/MS analyses, the molecular formula and constituents are validated. The sample mobility enables straightforward characterization of 1A, 1B, and 2A in relation to their stereo‐ and regio‐configurations, obviating the need for extensive time or sample quantities. Integration of findings from X‐ray crystallography, NMR, and IM‐MS furnishes precise and comprehensive structural insights into the coordination complexes.
Synthesis, characterization, and a comparative study on the catalytic activity of the functionalized magnetic nanoparticles for the green oxidation of sulfides
Authors: Rayati Saeed , Mazraeh Mohammad , Shokoohi Saeedeh , Nafarieh Parinaz , Nejabat Fatemeh
Published: July 2024

In the following research, meso‐tetrakis(4‐carboxyphenyl)porphyrinatomanganese (III) acetate (MnTCPP(OAc)) was immobilized onto the surface of two functionalized magnetic nanoparticles (MNPs) with different linker length via amide bond (Fe3O4@SiO2‐NH2‐MnTCPP(OAc) (A1) and Fe3O4@SiO2‐NHCO‐NH2‐MnTCPP(OAc) (A2)). The prepared catalysts were characterized by standard methods such as: atomic absorption spectroscopy (AAS), Ultra violet‐visible (UV‐Vis), Fourier‐transform infrared (FT‐IR), vibrating sample magnetometry (VSM), field emission scanning electron microscopy (FE‐SEM) and transmission electron microscopy (TEM) techniques and also the effect of the length of the linker on the catalytic activity of supported magnetic nanohybrids was investigated. The prepared magnetic nano‐catalysts with different linker lengths were participated in a comparative study of the clean oxidation of sulfides with urea hydrogen peroxide (UHP) in ethanol. The higher catalytic activity, selectivity and also reusability were achieved in the presence of the catalyst with longer linker length (A2) at ambient temperature. Moreover, the turnover number (TON) of catalytic reactions using A2 as catalyst was highly enough to consider the catalyst as an efficient material to oxidize various sulfides. Both A1 and A2 catalysts simply have accomplished 5 runs of recycling without significant decrease in the catalytic activity.
Influence of nutrition during previtellogenesis on the follicular development in the Asian Tiger mosquito, Aedes albopictus (Skuse) (Diptera: Culicidae)
Authors: Yamany Abeer S. , Abdel‐Gaber Rewaida
Published: July 2024

Dengue fever poses a global public health threat, with 2.5 billion people at risk of infection each year. Because the Aedes albopictus is the primary vector of dengue, it is closely monitored and handled. The efficiency of Dengue eradication is strongly dependent on understanding a female mosquito's physiological age. This study addresses key entomological issues about the impact of previtellogenic nutrition on egg production mechanisms. Ovarian development included two distinct periods: previtellogenesis and vitellogenesis. Sugar intake during previtellogenesis influences the size of the blood meal. The major parameter influencing the vitellogenesis process is the presence of a hematophagous feeding event following sugar concentration. Upon subjecting female mosquitoes to sucrose, the ovarian follicles entered the third phase of previtellogenesis. Once females feed on blood following sucrose, ovarian development enters the vitellogenesis, and the oocyte cytoplasm reveals that the yolk granules are organized in one or two rows like a crown, increasing oocyte size. Females fed 15% sucrose before a blood meal, have the largest vitellogenic growth, and follicular size, which is seven times greater than those fed water only. Fecundity increased by 78.7% by adding 7% sucrose to the diet. Mitochondria within oocytes increase, most likely due to their transportation from the nurse cells, where the yolk is synthesized. This study describes in detail the histological alterations detected in the ovaries during the previtellogenesis as well as those associated with yolk formation, suggesting that yolk protein deposition in the oocyte is associated with blood meal, independent of sucrose feeding.

Research Highlights

Adult nutrition during previtellogenesis significantly impacts various biological parameters and the physiological age of adults of Aedes albopictus.

Female mosquitoes experienced significant growth in vitellogenic development, vectorial capacity, and follicular size after consuming a diet with 15% sucrose before a blood meal.
Australians with metabolic dysfunction‐associated steatotic liver disease have a twofold increase in the incidence of cancer
Authors: Powell Elizabeth E , Roche Shruti , Sarraf Babak , Hartel Gunter , Skoien Richard , Leggett Barbara , O'Beirne James , Valery Patricia C
Published: July 2024

Background and Aim

Metabolic dysfunction‐associated steatotic liver disease (MASLD) is associated with an increased risk of extrahepatic morbidity. We compared the incidence of cancers in adults admitted to Queensland hospitals with MASLD with that for the Queensland population and examined the association between cirrhosis and type 2 diabetes and the development of extrahepatic cancers.

Methods

In this retrospective study, we identified all cancers (Queensland Cancer Registry) after the first hospitalization with MASLD during Jul‐2007 to Dec‐2019, estimated age‐standardized incidence (ASI) of cancers, and compared that with the ASI in the Queensland population (incidence rate ratios [IRR]). Among the MASLD cohort, we examined the association between diabetes and cancer risk (Cox regression). Median follow‐up was 3.8 years (54 204 person‐years).

Results

Totally 1104 new cancers were diagnosed in 1018 patients (8.9% of 9771 non‐cirrhotic and 1712 adults with cirrhosis). The ASI (all cancers) of 1668.2 per 100 000 person‐years in men (95% CI 1523.7–1827.4) and 1284.0 per 100 000 person‐years in women (95% CI 1169.6–1408.2) was 2‐fold higher than that of the Queensland population (IRR = 1.94, 95% CI 1.75–2.16 and IRR = 1.99, 95% CI 1.78–2.22, respectively). Incidence of stomach cancer, unknown primary, and pancreas was 3‐ to 5‐fold higher compared to the general population (all P < 0.001). In multivariable analysis of the MASLD cohort, older age (e.g. ≥70 years adjusted hazard ratio [adj‐HR] = 4.59, 95% CI 3.61–5.83), male gender (adj‐HR = 1.20, 95% CI 1.05–1.37), and cirrhosis (adj‐HR = 1.37, 95% CI 1.11–1.70) were independently associated with extrahepatic cancer risk, while diabetes was not.

Conclusions

Our findings will help to raise awareness among clinicians about the importance of cancer vigilance in this patient group.
Low HIV drug resistance prevalence among recently diagnosed HIV‐positive men who have sex with men in a setting of high PrEP use
Authors: King Jonathan M , Giallonardo Francesca Di , Shaik Ansari , McGregor Skye , Yeung Julie Yuek Kee , Sivaruban Tharshini , Lee Frederick J , Cunningham Philip , Dwyer Dominic E , Nigro Steven J , Grulich Andrew E , Kelleher Anthony D
Published: July 2024

Introduction

New South Wales (NSW) has one of the world's highest uptake rates of HIV pre‐exposure prophylaxis (PrEP). This uptake has been credited with sharp declines in HIV transmission, particularly among Australian‐born gay and bisexual men. Concerns have been raised around the potential for the emergence of tenofovir (TFV) and XTC (lamivudine/emtricitabine) resistance in settings of high PrEP use. Such an emergence could also increase treatment failure and associated clinical outcomes among people living with HIV (PLHIV). Despite low levels of nucleoside reverse‐transcriptase inhibitor (NRTI) resistance relating to PrEP use in clinical settings, there are few published studies describing the prevalence of NRTI resistance among people newly diagnosed with HIV in a setting of high PrEP use.

Methods

Using HIV antiretroviral drug resistance data linked to NSW HIV notifications records of people diagnosed from 1 January 2015 to 31 December 2021 and with HIV attributed to male‐to‐male sex, we described trends in TFV and XTC resistance. Resistance was identified using the Stanford HIV Drug Resistance genotypic resistance interpretation system. To focus on transmitted drug resistance, resistance prevalence estimates were generated using sequences taken less than 3 months post‐HIV diagnosis. These estimates were stratified by timing of sequencing relative to the date of diagnosis, year of sequencing, birthplace, likely place of HIV acquisition, and stage of HIV at diagnosis.

Results

Among 1119 diagnoses linked to HIV genomes sequenced less than 3 months following diagnosis, overall XTC resistance prevalence was 1.3%. Between 2015 and 2021, XTC resistance fluctuated between 0.5% to 2.9% and was 1.0% in 2021. No TFV resistance was found over the study period in any of the sequences analysed. Higher XTC resistance prevalence was observed among people with newly acquired HIV (evidence of HIV acquisition in the 12 months prior to diagnosis; 2.9%, p = 0.008).

Conclusions

In this Australian setting, TFV and XTC resistance prevalence in new HIV diagnoses remained low. Our findings offer further evidence for the safe scale‐up of PrEP in high‐income settings, without jeopardizing the treatment of those living with HIV.
Cost‐effectiveness analysis of community‐led HIV self‐testing among key populations in Côte d'Ivoire, Mali, and Senegal
Authors: Lu Ingrid Jiayin , Silhol Romain , d'Elbée Marc , Boily Marie‐Claude , Soni Nirali , Ky‐Zerbo Odette , Vautier Anthony , Simo Fosto Artlette , Badiane Kéba , Traoré Metogara , Terris‐Prestholt Fern , Larmarange Joseph , Maheu‐Giroux Mathieu ,   for the ATLAS Team
Published: July 2024

Introduction

HIV self‐testing (HIVST) is a promising strategy to improve diagnosis coverage among key populations (KP). The ATLAS (Auto Test VIH, Libre d'Accéder à la connaissance de son Statut) programme implemented HIVST in three West African countries, distributing over 380,000 kits up between 2019 and 2021, focussing on community‐led distribution by KP to their peers and subsequent secondary distribution to their partners and clients. We aim to evaluate the cost‐effectiveness of community‐led HIVST in Côte d'Ivoire, Mali and Senegal.

Methods

An HIV transmission dynamics model was adapted and calibrated to country‐specific epidemiological data and used to predict the impact of HIVST. We considered the distribution of HIVST among two KP—female sex workers (FSW), and men who have sex with men (MSM)—and their sexual partners and clients. We compared the cost‐effectiveness of two scenarios against a counterfactual without HIVST over a 20‐year horizon (2019–2039). The ATLAS‐only scenario mimicked the 2‐year implemented ATLAS programme, whereas the ATLAS‐scale‐up scenario achieved 95% coverage of HIVST distribution among FSW and MSM by 2025 onwards. The primary outcome is the number of disability‐adjusted life‐years (DALY) averted. Scenarios were compared using incremental cost‐effectiveness ratios (ICERs). Costing was performed using a healthcare provider's perspective. Costs were discounted at 4%, converted to $USD 2022 and estimated using a cost‐function to accommodate economies of scale.

Results

The ATLAS‐only scenario was highly cost‐effective over 20 years, even at low willingness‐to‐pay thresholds. The median ICERs were $126 ($88–$210) per DALY averted in Côte d'Ivoire, $92 ($88–$210) in Mali and 27$ ($88–$210) in Senegal. Scaling‐up the ATLAS programme would also be cost‐effective, and substantial epidemiological impacts would be achieved. The ICERs for the scale‐up scenario were $199 ($122–$338) per DALY averted in Côte d'Ivoire, $224 ($118–$415) in Mali and $61 ($18–$128) in Senegal.

Conclusions

Both the implemented and the potential scale‐up of community‐led HIVST programmes in West Africa, where KP are important to overall transmission dynamics, have the potential to be highly cost‐effective, as compared to a scenario without HIVST. These findings support the scale‐up of community‐led HIVST to reach populations that otherwise may not access conventional testing services.
Alveolar Soft Tissue Sarcoma: Correlation of MRI Features With Histological Grading and Patient Prognosis
Authors: Yuan Junhui , Xie Deshun , Fang Shaobo , Meng Fan , Shan Dongqiu , Wang Yuanyuan , Du Xinhui , Xu Chunmiao , Zhang Renzhi , Chen Xuejun
Published: July 2024

Background

Alveolar Soft Part Sarcoma (ASPS) is a rare, aggressive cancer whose diagnosis and treatment depend on histological grading. However, tumor variability can lead to underestimation, affecting treatment, and patient survival.

Objective

To evaluate MRI features associated with Grade III ASPS and to determine the relationship between MRI features and patient prognosis.

Study Type

Retrospective analysis.

Subjects

Sixty‐seven patients with ASPS were included with 37 males and 30 females (M/F = 1.23) follow‐up and survival analysis on 50 patients.

Field Strength/Sequence

A 3.0 T, T1WI‐FSE, T2WI‐FSE, DWI‐EPI, DCE‐MRI (gradient echo).

Assessment

MRI features (margin, peritumoral oedema, peritumoral enhancement, necrosis, vascular flow void signal, heterogeneous signal intensity [SI] at T1WI and T2WI, ADCmean, time‐intensity curve [TIC] type, distant metastasis, and bone invasion) and histological grading were independently evaluated by three radiologists and two pathologists, with Grade III considered high‐grade.

Statistical Tests

The chi‐square or Fisher's exact test was used to assess the correlation between MRI features and histological grading. Multivariable binary logistic regression identified independent factors associated with high‐grade tumors. The Kaplan–Meier method and Cox proportional hazards model were used to calculate hazard ratios for MRI features.

Results

Tumor necrosis, heterogeneous SI at T2WI ≥50%, and ADCmean were associated with high‐grade ASPS. Tumor necrosis was an independent factors associated with local relapse‐free survival (odds ratio [OR], 3.88). TIC type was associated with 5‐year survival rate (OR, 2.80) and local relapse‐free survival (OR, 2.69). Heterogeneous SI at T2WI ≥50% was associated with 5‐year survival (OR, 4.00), local relapse‐free survival (OR, 5.58), and local relapse‐free survival (OR, 4.84).

Data Conclusion

MRI features including tumor necrosis, heterogeneity of SI at T2WI, ADCmean, and TIC type aid in assessing ASPS grading and prognosis.

Evidence Level

4

Technical Efficacy

Stage 5
MicroRNA‐26a deficiency attenuates the severity of frozen shoulder in a mouse immobilization model
Authors: Sumimoto Yasuhiko , Harada Yohei , Yimiti Dilimulati , Watanabe Chikara , Miyaki Shigeru , Adachi Nobuo
Published: July 2024

The main pathogenesis of the frozen shoulder is thought to be the inflammation of the intra‐articular synovium and subsequent fibrosis of the shoulder joint capsule. However, the molecular pathogenesis of the frozen shoulder is still unknown. A class of noncoding RNAs, microRNAs contribute to various diseases including musculoskeletal diseases. MicroRNA‐26a (miR‐26a) has been reported to be associated with fibrosis in several organs. This study aims to reveal the role of miR‐26a on fibrosis in the shoulder capsule using a frozen shoulder model in miR‐26a deficient (miR‐26a KO) mice. MiR‐26a KO and wild‐type (WT) mice were investigated using a frozen shoulder model. The range of motion (ROM) of the shoulder, histopathological changes such as synovitis, and fibrosis‐related gene expression in the model mice were evaluated to determine the role of miR‐26a. In WT mice, both inflammatory cell infiltration and thickening of the inferior shoulder joint capsule were observed after 1 week of immobilization, and this thickening further progressed over the subsequent 6 weeks. However, the immobilized shoulder in miR‐26a KO mice consistently exhibited significantly better ROM compared with WT mice at 1 and 6 weeks, and histological changes were significantly less severe. The expression of inflammation‐ and fibrosis‐related genes was decreased in the miR‐26a KO mice compared with WT mice at 1 and 6 weeks. Together, miR‐26a deficiency attenuated the severity of frozen shoulder in the immobilization model mouse. The present study suggests that miR‐26a has the potential to be a target miRNA for therapeutic approach to frozen shoulder.
Depth perception optimization of mixed reality simulation systems based on multiple‐cue fusion
Authors: Wang Wei , Chen Tong , Liu Haiping , Zhang Jiali , Wang Qingli , Jiang Qinsheng
Published: July 2024

Mixed reality technology can be applied to simulation training, improve surgical performance, enhance 3D game experience, and so on, attracting extensive attention of researchers. The perception of the user about head‐mounted display MR using HoloLens is critical, especially for precision applications such as virtual hoisting. Designing and adding appropriate depth cues in MR scenes is an effective way to improve users' depth perception. In this study, taking virtual hoisting training system as an example, the depth perception strategy of multi‐cue fusion is proposed to improve the perception effect. Based on the mechanism of human depth perception, five kinds of depth cues are designed. The depth perception effect of adding single cue is studied by perceptual matching experiment. Based on the principle of fuzzy clustering, a multiple‐cue comprehensive depth optimization strategy on viewing distance scale is proposed. Finally, the perceptual matching results demonstrate the effectiveness of the multi‐cue fusion strategy, and the average error is reduced by 20.68% compared with the single‐cue strategy, which can significantly improve the spatial depth perception. This research can provide a reference for improving users' depth perception in interactive MR simulation systems.
Magnetic molecularly imprinted polymer based on coordination for the determination of trace banned substance furosemide in human urine
Authors: He Kunlin , Chen Qian , Chen Xueling , Zhang Chungu , Feng Shun , Shan Lianhai
Published: July 2024

Furosemide (FUR), banned in sports events by the World Anti‐Doping Agency, is a key target in drug tests, necessitating a pretreatment material capable of selectively, rapidly, and sufficiently separating/enriching analytes from complex matrices. Herein, a metal‐mediated magnetic molecularly imprinted polymer (mMIP) was rationally designed and synthesized for the specific capture of FUR. The preparations involved the utilization of chromium (III) as the binding pivot, (3‐aminopropyl)triethoxysilane as functional monomer, and Fe3O4 as core, all assembled via free radical polymerization. Both the morphologies and adsorptive properties of the mMIP were characterized using multiple methods. The resulting Cr(III)‐mediated mMIP (ChM‐mMIP) presented excellent selectivity and specificity toward FUR. Under optimized conditions, the adsorption capacity reached 128.50 mg/g within 10 min, and the imprinting factor was 10.41. Moreover, it was also successfully applied as a dispersive solid‐phase extraction material, enabling the detection of FUR concentration as low as 20 ng/mL in human urine samples when coupled with a high‐performance liquid chromatography/photodiode array. Overall, this study offers a valuable strategy for the development of novel recognition material.
Indirect and sensitive determination of microRNAs by magnetic field‐assisted capillary sieving electrophoresis combined with catalytic hairpin assembly
Authors: Ning Jinfeng , Ye Junlan , Wang Qingqing , Wang Wei
Published: July 2024

To determine multiple microRNAs (miRNAs) from cells simultaneously is essential for understanding biological functions. Capillary electrophoresis (CE) can simultaneously determine multiple miRNAs by separation. Nevertheless, similar lengths and low concentrations in cells make miRNAs hard to separate and detect. In this study, CE with laser‐induced fluorescence detection was combined with catalytic hairpin assembly (CHA) to determine three miRNAs, miR‐21, miR‐31, and miR‐122. The amplification products of CHA, which were DNA duplexes, were designed to have different lengths for different miRNAs. This allowed for easy separation of the duplexes of different miRNAs by CE. The indirect determination of miRNAs was then achieved by separating and detecting these duplexes. A magnetic field was first applied on the capillary sieving electrophoresis to assist in the separation of the duplexes. Under the optimal conditions, the three duplexes could be completely separated within 2.5 min with the detection limits of miRNAs in the range 1.12–4.05 × 10−15 M. MiR‐21 and miR‐31 were successfully determined from Hela cells, while miR‐122 was determined from chicken livers by this method. The recoveries ranged from 97.5% to 118%. The developed method was sensitive and reliable for miRNA determination.
Multiple analyses of main flavor components in reconstituted tobacco and transfer behavior of their key substances during heating
Authors: Shi Jianyang , Yang Yunxia , Zhang Ting , Liang Kun , Guo Linqing , Deng Ruijie , Liu Kai , Ren Yao
Published: July 2024

Reconstituted tobacco (RT) is a product made by reprocessing tobacco waste, experiencing a growing demand for heat‐not‐burn products. The purpose of this study is to analyze the main flavor ingredients in RT aerosol, as well as the transfer behavior of key flavor substances from substrates to aerosol and the concentrations of these compounds in the substrate after heating. First, we demonstrated that the odor of four RT aerosol samples could be distinguished using an electronic nose. Through non‐targeted analysis, 93 volatile compounds were detected by gas chromatography‐mass spectrometry, and 286 non/semi‐volatile compounds were identified by ultra‐high‐performance liquid electrophoresis chromatography‐mass spectrometry in aerosol. Furthermore, we found that the formation of RT aerosol involves primarily evaporation and distillation, however, the total content delivered from unheated RT samples to aerosol remains relatively low due to compound volatility and cigarette filtration. Thermal reactions during heating indicated the pyrolysis of chlorogenic acid to generate catechol and resorcinol, while Maillard reactions involving glucose and proline produced 2,3‐dihydro‐3,5‐dihydroxy‐6‐methyl‐4h‐pyran‐4‐one. The study highlighted that heating RT at approximately 300°C could mitigate the production of harmful substances while still providing a familiar sensory experience with combusted tobacco.
Rapid identification of chemical components and screening of acetylcholinesterase inhibitors from Dalbergia odorifera based on mass defect and diagnostic ion filtering strategy, affinity ultrafiltration, and liquid chromatography‐tandem mass spectrometry
Authors: Zhang Hongbin , Liu Yuecheng , Zhang Ling , Tian Zhenhua , Zhang Hui , Jiang Haiqiang
Published: July 2024

Dalbergia odorifera is a natural product rich in pharmacological ingredients, but the comprehensive characterization and rapid profiling of active components remain a challenge. Thus, an integrated data mining and identification strategy was exploited to efficiently identify the chemical constituents and screen acetylcholinesterase inhibitors (AChEIs) through affinity ultrafiltration and ultra‐high‐performance liquid chromatography‐mass spectrometry (AUF‐UHPLC‐MS). As a result, polygonal mass defect filtering, diagnostic product ions, and neutral loss rules were created for rapid structural classification and component identification. A total of 140 flavonoids were tentatively characterized, including 41 isoflavonoids, 23 flavanones, 21 isoflavans, 19 flavones and flavonols, 13 neoflavonoids, 11 isoflavanones, seven flavone glycosides, and five chalcones. Subsequently, six natural AChEIs including tectorigenin, fisetin, dalbergin, pterostilbene, isoliquiritigenin, and biochanin A were screened out using AUF‐UHPLC‐MS and molecular docking. Meanwhile, the AChE inhibitory activities of the six compounds were assessed in vitro, tectorigenin, fisetinand, and dalbergin have moderate inhibitory activity. In conclusion, a novel strategy for systematic characterization and further screening of active compounds in natural products was established, which provides a material basis for quality control of Dalbergia odorifera.
Evaluation and comparison of liquid chromatography/high‐resolution mass spectrometry platforms for the separation and characterization of ginsenosides from the leaves of Panax ginseng
Authors: Li Xiaohang , Zou Yadan , Cheng Huizhen , Ding Mengxiang , Yang Yang , Hong Lili , Xiong Ying , Zhang Min , Li Xue , Chen Qinhua , Wang Hongda , Cui Yuanwu , Yang Wenzhi
Published: July 2024

The measurement of data repeatability in small‐molecule metabolites acquired within and among different liquid chromatography‐mass spectrometry (LC‐MS) platforms is crucial for data sharing or data transfer in natural products research. This work was designed to investigate and evaluate the separation and detection performance of three commercial high‐resolution LC‐MS platforms (e.g., Agilent 6550 QTOF, Waters Vion IM‐QTOF, and Thermo Scientific Orbitrap Exploris 120) using 68 ginsenoside references and the extract of Panax ginseng leaf. The retention time (tR), measured on these three platforms (under the same chromatography condition), showed good stability in different concentration tests, and within/among different instruments for both intra‐day and inter‐day precision examinations. Correlation in tR of ginsenosides was also highly determined on these three platforms. In spite of the different mass analyzers involved, these three platforms gave the accurate mass determination ability, especially enhanced resolution gained because of the ion mobility (IM) separation facilitated by IM‐quadrupole time‐of‐flight. The current study has systematically evaluated the separation and MS detection performance enabled by three high‐resolution LC‐MS platforms taking ginsenosides as the template, and the reported findings can benefit the researchers for the selection of analytical platforms and the purpose of data sharing or data transfer.
High‐Speed Short Infrared Detector Based on Vertical Gr/Se0.2Te0.8/GaAs Heterojunction
Authors: Yang Chong , Yu He , Lian Yunlu , Liu Yiming , Wu Maoyi , Yang Xiutao , Han Jiayue , Dong Xiang , Gou Jun , Zheng Xing , Wu Zhiming , Jiang Yadong , Wang Jun
Published: July 2024

In the domain of high‐performance short‐wave infrared (SWIR) photodetection and imaging, existing technologies predominantly utilize single‐crystal germanium and III‐V semiconductors. Despite their efficacy, these materials are encumbered by laborious synthesis and complex fabrication demands. In this study, the synthesis of large‐area, high‐crystallinity Se0.2Te0.8 thin films through a CMOS‐compatible vacuum thermal evaporation process is reported. A high‐speed, broad‐spectrum photodetector engineered with an innovative Gr/Se0.2Te0.8/GaAs vertical heterostructure is presented, which capitalizes on the augmented carrier mobility and employs graphene innovatively as both a carrier collection interface and an electrode. This configuration facilitates a remarkably swift response time of 800 ns/1 µs at the crucial 1310 nm wavelength for optical communications. Moreover, the fabrication of a 5 × 5 array device demonstrates substantial SWIR imaging capabilities at ambient conditions, marking a paradigm shift in uncooled infrared imaging and communication technologies. This work not only extends the boundaries of SWIR photodetector performance but also underscores the potential of novel material systems in high‐speed optical applications.
Efficient Manipulation of Near‐Field Terahertz Waves: Individually Addressable Transmissive Meta‐Device
Authors: Wang Peng , Fu Xiaojian , Yang Jun , Fu Yuan , Cai Qingdong , Liu Yujie , Yang Silei , Shi Jianshu , Zheng Shilie , Cui Tie Jun
Published: July 2024

Due to the powerful capability in manipulating electromagnetic (EM) waves, digital coding and programmable metasurfaces have found vast application prospects across numerous areas such as next‐generation wireless communications and digital holography. Liquid crystals (LCs), as dielectric materials with significant birefringence effect over a wide frequency range, provide a cost‐effective solution for achieving the programmable metasurfaces and flexible EM manipulations, especially in the terahertz (THz) band. Different from the conventional 1D control and single functionality of transmissive LC‐based devices, here, a 16 × 16 addressable amplitude‐phase coding meta‐device is proposed to support for frequency multiplexing by using the film on glass (FOG) technology. Both numerical simulations and experimental results demonstrate that the meta‐atom exhibits an amplitude modulation depth over 90% and a phase tuning range ≈180° at two distinct frequencies, and hence the single meta‐device can provide multifunctional EM applications, including near‐field printing imaging, 3D THz energy convergence, and zero‐order Bessel beam generation. The proposed strategy paves a way for constructing highly integrated and high‐performance THz multifunctional information processing systems.
Biodegradable Magnetic Vesicles for Magnetic Hyperthermia Stimulated Drug Release
Authors: Li Xin , Liu Ya , Chen Xing , Zhao Chenxi , Huang Mengya , Shan Kui , Zhou Xun , Liu Xing
Published: July 2024

Magnetic nanomaterials have emerged as an effective drug‐delivery platform for targeted imaging and therapy. However, it remains challenging to fabricate biodegradable magnetic nanoparticles with controllable drug release behaviors and strong magnetic responsiveness. Here an asiaticoside‐loaded biodegradable magnetic vesicle is developed based on the self‐assembly of amphiphilic block copolymer tethered superparamagnetic iron oxide nanoparticles. Thanks to the collective properties, the magnetic vesicles show stronger magnetic responsiveness than individual nanoparticles. Additionally, the magnetic vesicles are dissociated within weeks owing to the biodegradable polymer backbone, which can significantly improve the long‐term biocompatibility of the nanomaterials. The asiaticoside‐loaded magnetic vesicles can readily release the payloads in an alternating magnetic field, likely due to the rising local temperature over the phase transition temperature of the polymers attached on nanoparticles. This work provides new insights into the design and construction of biodegradable magnetic nanomedicines for targeted drug delivery.
Correlation Between Side‐Chain Arrangement and Main‐Chain Helix Conformation in Liquid Crystalline Syndiotactic Poly(Substituted Methylene)s Bearing Cyanobiphenyl Mesogens
Authors: Kiyoura Masamichi , Koshimizu Noboru , Shikinaka Kazuhiro , Shigehara Kiyotaka , Tokita Masatoshi
Published: July 2024

This study focuses on the helical conformation of the main chain of syndiotactic poly(substituted methylene)s with 4‐cyanobiphenyl (CB) moieties incorporated at the ends of the alkyloxycarbonyl side chains. The polymers are designated as PCBn, where n is the number of methylene units in the spacer between the main chain and CB. PCB6 and PCB8 assemble the side chains into layers without positional order within the layers. Meanwhile, PCB10 and PCB12 assemble the side chains into layers with a short‐range positional order. Each CB moiety at a side chain end is bonded to a carbon atom, which is arranged along the helical main chain contour, and its position correlates with that of the carbon atom. As the temperature increases, PCB10 undergoes a phase transition, losing the positional order of the side chains within a layer, which alters the main chain conformation. Consequently, the positions of the side‐chain CB moieties affect the helical conformation of the main chain. In contrast, PCB12 maintains the main chain conformation when undergoing a phase transition similar to that of PCB10. PCB12 with the longer spacer allows the main chain to adopt a helix conformation, which is independent of the positions of side‐chain CB moieties.
Development of Ductile‐Sticky Bone Fillers from Biodegradable Hydrolyzed Wool‐Keratin and Silk Fibroin
Authors: Bekar Serife , Sezgin Arslan Tugba , Arslan Yavuz Emre
Published: July 2024

In the present study, a method is proposed for preparing novel ductile‐sticky materials that can be used as bone void fillers using hydrolyzed wool‐keratin (WK) and silk fibroin (SF). This methodology uses citric acid as a cross‐linking agent in preparing keratin paste (KP) owing to its non‐toxicity and plasticizing properties. The Keratin paste‐silk fibroin structure (KPSF) is obtained by adding SF, which possesses biocompatible and superior mechanical properties. Methanol treatment is employed on the KPSF mixture to convert the Silk I structure in the SF to Silk II, resulting in a water‐insoluble and tightly packed proteinaceous structure. The physicochemical properties of both bioscaffolds are investigated and discussed in detail by comparison. Based on the findings, the presence of SF in the KPSF structure contributed to properties such as flexibility and porosity. In ovo CAM analysis reveals that both materials exhibit proangiogenic properties and are biocompatible. KP and KPSF bioscaffolds can be converted into ductile‐sticky forms by adding water. It believes that these forms can easily apply to bone defect areas, particularly cavitary bone defects. Furthermore, KPSF bioscaffolds, with better mechanical properties, can be considered candidates for use in non‐load‐bearing bone tissue engineering applications.
Single‐cell RNA sequencing reveals reduced intercellular adhesion molecule crosstalk between activated hepatic stellate cells and neutrophils alleviating liver fibrosis in hepatitis B virus transgenic mice post menstrual blood‐derived mesenchymal stem cell transplantation
Authors: Chen Lijun , Huang Yuqi , Zhang Ning , Qu Jingjing , Fang Yangxin , Fu Jiamin , Yuan Yin , Zhang Qi , Li Hang , Wen Zuoshi , Yuan Li , Chen Lu , Xu Zhenyu , Li Yifei , Yan Huadong , Izawa Hiromi , Li Lanjuan , Xiang Charlie
Published: July 2024

Liver fibrosis can cause hepatitis B virus (HBV)‐associated hepatocellular carcinoma. Menstrual blood‐derived mesenchymal stem cells (MenSCs) can ameliorate liver fibrosis through paracrine. Single‐cell RNA sequencing (scRNA‐seq) may be used to explore the roadmap of activated hepatic stellate cell (aHSC) inactivation to target liver fibrosis. This study established HBV transgenic (HBV‐Tg) mouse model of carbon tetrachloride (CCl4)‐induced liver fibrosis and demonstrated that MenSCs migrated to the injured liver to improve serological indices and reduce fibrotic accumulation. RNA‐bulk analysis revealed that MenSCs mediated extracellular matrix accumulation and cell adhesion. Liver parenchymal cells and nonparenchymal cells were identified by scRNA‐seq in the control, CCl4, and MenSC groups, revealing the heterogeneity of fibroblasts/HSCs. A CellChat analysis revealed that diminished intercellular adhesion molecule (ICAM) signaling is vital for MenSC therapy. Specifically, Icam1 in aHSCs acted on Itgal/Itgb2 and Itgam/Itgb2 in neutrophils, causing decreased adhesion. The expression of Itgal, Itgam, and Itgb2 was higher in CCl4 group than in the control group and decreased after MenSC therapy in neutrophil clusters. The Lcn2, Pglyrp1, Wfdc21, and Mmp8 had high expression and may be potential targets in neutrophils. This study highlights interacting cells, corresponding molecules, and underlying targets for MenSCs in treating HBV‐associated liver fibrosis.
Risk of Dementia in Parkinson's Disease: A Systematic Review and Meta‐Analysis
Authors: Gibson Lucy L. , Weintraub Daniel , Lemmen Roos , Perera Gayan , Chaudhuri Kallol Ray , Svenningsson Per , Aarsland Dag
Published: July 2024

Estimates of the risk of dementia in Parkinson's disease (PDD) vary widely. We aimed to review the incidence of PDD and in a meta‐analysis estimate the pooled annual incidence and relative risk of PDD while also exploring factors that may contribute to heterogeneity between studies. Preferred Reporting Items for Systematic reviews and Meta‐Analyses guidelines were followed and MEDLINE and EMBASE were searched for articles reporting the number of cases of dementia in a population, followed longitudinally, with a minimum of 100 dementia‐free Parkinson's disease (PD) patients at baseline. Meta‐analyses and meta‐regressions were used to estimate the pooled incidence rate of PDD and the relative risk of PDD versus healthy controls (HC). A total of 32 studies were identified, 25 reporting the incidence of PDD and 10 reporting the relative risk of PDD versus HC. The pooled incidence rate of PDD was 4.45 (95% confidence interval [CI], 3.91–4.99) per 100 person‐years at risk, equating to a 4.5% annual risk of dementia in a PD prevalent population. The relative risk of PDD was estimated to be 3.25 (95% CI, 2.62–4.03) times greater than HC. Factors contributing to study heterogeneity and disparities in the estimated risk of PDD include the age of patients, year of recruitment, and study location. Significant gaps remain with no studies identified in several geographical regions. Future studies should stratify by age and standardize reporting to reduce overall heterogeneity. © 2024 The Author(s). Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
Gen Z chooses trade schools over traditional colleges
Authors: Hodges Dawn Z.
Published: July 2024

Over the past several months, there has been a lot in the news about students from Generation Z (1997–2012) choosing to attend “trade” schools over more traditional colleges. Those of us who work in the trade schools don’t call them that. The Technical College System of Georgia is, I suppose, what others might call trade schools. The colleges in the system offer such programs as welding, automotive maintenance and repair, plumbing, construction, industrial systems maintenance, electrical lineman, aviation maintenance, among many others.
Density functional theory study on frustrated Lewis pairs catalyzed C‐H activation of heteroarenes: Mechanism variation tuning by electronic effect
Authors: Shao Youxiang , Xiao Kang , Wang Huize , Liu Yalan
Published: July 2024

Unreactive C‐H bond activation is a new horizon for frustrated Lewis pairs (FLPs) chemistry. Although concerted mechanism (Science 2015, 349, 513) and stepwise carbene mechanism (Org. Lett. 2018, 20, 1102) have been proposed for the FLPs catalyzed C‐H bond activation of 1‐methylpyrrole, the influence of electronic properties of FLPs on the reaction mechanism is far away from well‐understood. In this study, an assortment of P‐B type FLPs with different electronic characteristic was employed to study the catalyzed C‐H bond activation of 1‐methylpyrrole by using density functional theory calculations. Detailed calculations demonstrated that the reactivity variation and the reaction mechanism binary of FLPs catalyzed C‐H activation can be varied by tuning electronic effect of Lewis base center. On the one hand, the concerted C‐H activation reactivity is mainly controlled by the electron donation of the lone pair of Lewis base center; thus, the FLPs with electron‐donating substituents (FLP1, FLP2, and FLP3) catalyzed the C‐H bond activation through concerted mechanism. On the other hand, the reactivity of stepwise carbene mechanism is mostly attributed to the vacant orbital of Lewis acid center; as a result, the FLP5 bearing ‐P(C6F5)2 preferred to catalyzed the bond activation through concerted mechanism. In contrast, a metathesis mechanism through strained four‐membered ring transition state is less feasible. These results should provide deeper insight and broader perspective to understand the structure and function of FLPs for rational design of FLPs catalyzed C‐H bond activation.