bluuebunny/crossref_metadata_2025 huggingface dataset:
The Unknown Role of Periostin in Psoriatic Epidermal Hyperplasia
Authors: Wojciechowska Milena , Ścibior Kinga , Betyna-Białek Monika , Kostrzewska Ewa , McFarlane Oliwia
Published: November 2023

Psoriasis is an inflammatory skin disease that affects 1–2% of the general population. The pathomechanism is based on type 1 immunological reactions. Hyperplasia of the epidermis in psoriasis is a result of disrupted epidermal architecture due to increased synthesis and expression of extracellular matrix proteins. In our study, we analyzed the involvement of periostin (POSTN) in the pathogenesis of psoriasis, as one of the extracellular matrix proteins belonging to the fasciclin family. The study group consisted of 70 patients with psoriasis, while the control group comprised 30 healthy individuals. The serum concentrations of POSTN, Il-6, Il-17, Il-22, TNF-α and IFN-γ were measured in all participants. The severity of psoriasis was determined using the PASI (Psoriasis Area and Severity Index) score. The presence of POSTN in biopsy samples of 50 patients was assessed using the direct immunofluorescence method. The results were subjected to statistical analysis. The serum concentrations of POSTN, Il-6, Il-17, Il-22, TNF-α and IFN-γ in the study group are significantly higher than in the control group. Positive correlation has been demonstrated between the PASI score and the investigated cytokines, but not with POSTN. There was no statistically significant correlation between the POSTN level and the cytokines levels. POSTN deposits were localized in the epidermis in 66% of patients with psoriasis. The role of POSTN in the pathogenesis of psoriasis remains unclear. The mechanisms inducing the synthesis and expression of POSTN in psoriatic skin are not yet fully understood. Further research is needed to enhance our understanding of the mechanism underlying epidermal hyperplasia in psoriasis.
Garcinia cambogia Extract Increased Hepatic Levels of Lipolysis-Stimulated Lipoprotein Receptor and Lipids in Mice on Normal Diet
Authors: Hanse Marine , Akbar Samina , Layeghkhavidaki Hamed , Yen Frances T.
Published: November 2023

Garcinia cambogia extract (GCE) is a popular weight-loss supplement that also lowers plasma triglyceride (TG) levels. We hypothesized that GCE-mediated inhibition of ATP citrate lyase and thereby hepatic TG production could lead to compensatory mechanisms, including increased hepatic TG uptake via lipoprotein receptors. GCE (20 mg/day) administered 40 days orally to female C57BL/6Rj mice on a standard chow diet led to a decrease in both plasma fasting and post-prandial TG-rich lipoprotein levels, but with no significant change in body weight gain. Lipolysis stimulated lipoprotein receptor (LSR) protein levels, but not those of LDL-receptor, were increased as compared to controls. Mouse Hepa1-6 cells treated with the GCE active ingredient, hydroxycitrate, also led to increased LSR protein levels. Hepatic total cholesterol, TG, and muscle TG contents were higher in GCE-treated animals as compared to controls, whereas adipose TG levels were unchanged. LSR and LDL-receptor protein levels were correlated with liver total cholesterol, but only LDL-receptor was associated with liver TG. These results show that GCE treatment in mice on a standard chow diet led to significantly increased liver and muscle lipids, with no significant change in adipose tissue TG levels, which should be considered in the long-term use of GCE.
Frequency Evaluation of the Interleukin-6 −174G>C Polymorphism and Homeostatic Iron Regulator (HFE) Mutations as Disease Modifiers in Patients Affected by Systemic Lupus Erythematosus and Rheumatoid Arthritis
Authors: Carini Mattia , Fredi Micaela , Cavazzana Ilaria , Bresciani Roberto , Ferrari Fabiana , Monti Eugenio , Franceschini Franco , Biasiotto Giorgio
Published: November 2023

Autoimmune diseases are generally characterized by a multifactorial etiology and are often associated with a genetic predisposition. Both iron metabolism and the inflammatory cytokine system have been shown to play a pivotal role in the dysregulation of the immune response in many different autoimmune conditions, rheumatologic diseases included. The purpose of this work was to analyze the frequency of mutations altering the expression of IL-6 or influencing iron metabolism in patients affected by autoimmune diseases such as Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE). In this study, 144 patients were enrolled: 77 and 67 patients were affected by RA and SLE, respectively. In these cohorts, the frequency of the IL-6 polymorphism −174G>C located in the IL-6 gene promoter was tested. Moreover, the frequencies of the three HFE gene variations associated with iron overload were analyzed: p.His63Asp, p.Ser65Cys and p.Cys282Tyr. The two mutations p.His63Asp and p.Ser65Cys in the HFE gene did not reach statistical significance in any of the comparisons, regardless of the statistical model, cohorts of patients and control populations analyzed. The frequencies of the p.Cys282Tyr mutation and the IL-6 polymorphism −174G>C were found to be overall significantly decreased in RA and SLE patients when the Dominant model and Allele contrast were adopted with both the Odds Ratio and Chi-square. Although further investigation is needed, the examination of the frequencies of the −174G>C IL-6 promoter polymorphism and HFE mutations may add some valuable information on the interplay linking iron metabolism, inflammation and immunity in autoimmune diseases such as SLE and RA.
Gut Microbiome–Estrobolome Profile in Reproductive-Age Women with Endometriosis
Authors: Pai Angel Hsin-Yu , Wang Yi-Wen , Lu Pei-Chen , Wu Hsien-Ming , Xu Jia-Ling , Huang Hong-Yuan
Published: November 2023

Microbiota is associated with our bodily functions and microenvironment. A healthy, balanced gut microbiome not only helps maintain mucosal integrity, prevents translocation of bacterial content, and contributes to immune status, but also associates with estrogen metabolism. Gut dysbiosis and estrobolome dysfunction have hence been linked to certain estrogen-dependent diseases, including endometriosis. While prior studies on microbiomes and endometriosis have shown conflicting results, most of the observed microbial differences are seen in the genital tract. This case-control study of reproductive-age women utilizes their fecal and urine samples for enzymatic, microbial, and metabolic studies to explore if patients with endometriosis have distinguishable gut microbiota or altered estrogen metabolism. While gut β-glucuronidase activities, microbial diversity, and abundance did not vary significantly between patients with or without endometriosis, fecal samples of patients with endometriosis were more enriched by the Erysipelotrichia class and had higher folds of four estrogen/estrogen metabolites. Further studies are needed to elucidate what these results imply and whether there indeed is an association or causation between gut microbiota and endometriosis.
Investigating the Influence of Morphine and Cocaine on the Mesolimbic Pathway Using a Novel Microimaging Platform
Authors: Ganaway Austin , Tatsuta Kousuke , Castillo Virgil Christian Garcia , Okada Ryoma , Sunaga Yoshinori , Ohta Yasumi , Ohta Jun , Ohsawa Masahiro , Akay Metin , Akay Yasemin M.
Published: November 2023

Dopamine (DA)’s relationship with addiction is complex, and the related pathways in the mesocorticolimbic system are used to deliver DA, regulating both behavioral and perceptual actions. Specifically, the mesolimbic pathway connecting the ventral tegmental area (VTA) and the nucleus accumbens (NAc) is crucial in regulating memory, emotion, motivation, and behavior due to its responsibility to modulate dopamine. To better investigate the relationship between DA and addiction, more advanced mapping methods are necessary to monitor its production and propagation accurately and efficiently. In this study, we incorporate dLight1.2 adeno-associated virus (AAV) into our latest CMOS (complementary metal-oxide semiconductor) imaging platform to investigate the effects of two pharmacological substances, morphine and cocaine, in the NAc using adult mice. By implanting our self-fabricated CMOS imaging device into the deep brain, fluorescence imaging of the NAc using the dLight1.2 AAV allows for the visualization of DA molecules delivered from the VTA in real time. Our results suggest that changes in extracellular DA can be observed with this adapted system, showing potential for new applications and methods for approaching addiction studies. Additionally, we can identify the unique characteristic trend of DA release for both morphine and cocaine, further validating the underlying biochemical mechanisms used to modulate dopaminergic activation.
The Controversial Effect of Antibiotics on Methicillin-Sensitive S. aureus: A Comparative In Vitro Study
Authors: Hackemann Valeria C. J. , Hagel Stefan , Jandt Klaus D. , Rödel Jürgen , Löffler Bettina , Tuchscherr Lorena
Published: November 2023

Methicillin-sensitive Staphylococcus (S.) aureus (MSSA) bacteremia remains a global challenge, despite the availability of antibiotics. Primary treatments include β-lactam agents such as cefazolin and flucloxacillin. Ongoing discussions have focused on the potential synergistic effects of combining these agents with rifampicin or fosfomycin to combat infections associated with biofilm formation. Managing staphylococcal infections is challenging due to antibacterial resistance, biofilms, and S. aureus’s ability to invade and replicate within host cells. Intracellular invasion shields the bacteria from antibacterial agents and the immune system, often leading to incomplete bacterial clearance and chronic infections. Additionally, S. aureus can assume a dormant phenotype, known as the small colony variant (SCV), further complicating eradication and promoting persistence. This study investigated the impact of antibiotic combinations on the persistence of S. aureus 6850 and its stable small colony variant (SCV strain JB1) focusing on intracellular survival and biofilm formation. The results from the wild-type strain 6850 demonstrate that β-lactams combined with RIF effectively eliminated biofilms and intracellular bacteria but tend to select for SCVs in planktonic culture and host cells. Higher antibiotic concentrations were associated with an increase in the zeta potential of S. aureus, suggesting reduced membrane permeability to antimicrobials. When using the stable SCV mutant strain JB1, antibiotic combinations with rifampicin successfully cleared planktonic bacteria and biofilms but failed to eradicate intracellular bacteria. Given these findings, it is reasonable to report that β-lactams combined with rifampicin represent the optimal treatment for MSSA bacteremia. However, caution is warranted when employing this treatment over an extended period, as it may elevate the risk of selecting for small colony variants (SCVs) and, consequently, promoting bacterial persistence.
Buckwheat (Fagopyrum esculentum) Hulls Are a Rich Source of Fermentable Dietary Fibre and Bioactive Phytochemicals
Authors: Zhang Zhihong , Fan Songtao , Duncan Gary J. , Morris Amanda , Henderson Donna , Morrice Philip , Russell Wendy R. , Duncan Sylvia H. , Neacsu Madalina
Published: November 2023

Pseudo-cereals such as buckwheat (Fagopyrum esculentum) are valid candidates to promote diet biodiversity and nutrition security in an era of global climate change. Buckwheat hulls (BHs) are currently an unexplored source of dietary fibre and bioactive phytochemicals. This study assessed the effects of several bioprocessing treatments (using enzymes, yeast, and combinations of both) on BHs’ nutrient and phytochemical content, their digestion and metabolism in vitro (using a gastrointestinal digestion model and mixed microbiota from human faeces). The metabolites were measured using targeted LC-MS/MS and GC analysis and 16S rRNA gene sequencing was used to detect the impact on microbiota composition. BHs are rich in insoluble fibre (31.09 ± 0.22% as non-starch polysaccharides), protocatechuic acid (390.71 ± 31.72 mg/kg), and syringaresinol (125.60 ± 6.76 mg/kg). The bioprocessing treatments significantly increased the extractability of gallic acid, vanillic acid, p-hydroxybenzoic acid, syringic acid, vanillin, syringaldehyde, p-coumaric acid, ferulic acid, caffeic acid, and syringaresinol in the alkaline-labile bound form, suggesting the bioaccessibility of these phytochemicals to the colon. Furthermore, one of the treatments, EC_2 treatment, increased significantly the in vitro upper gastrointestinal release of bioactive phytochemicals, especially for protocatechuic acid (p < 0.01). The BH fibre was fermentable, promoting the formation mainly of propionate and, to a lesser extent, butyrate formation. The EM_1 and EC_2 treatments effectively increased the content of insoluble fibre but had no effect on dietary fibre fermentation (p > 0.05). These findings promote the use of buckwheat hulls as a source of dietary fibre and phytochemicals to help meet dietary recommendations and needs.
Influence of Lavender Essential Oil on the Physical and Antibacterial Properties of Chitosan Sponge for Hemostatic Applications
Authors: Gheorghiță Daniela , Antoniac Iulian , Moldovan Horațiu , Antoniac Aurora , Grosu Elena , Motelica Ludmila , Ficai Anton , Oprea Ovidiu , Vasile Eugeniu , Dițu Lia Mara , Raiciu Anca Daniela
Published: November 2023

Uncontrollable bleeding continues to stand as the primary cause of fatalities globally following surgical procedures, traumatic incidents, disasters, and combat scenarios. The swift and efficient management of bleeding through the application of hemostatic agents has the potential to significantly reduce associated mortality rates. One significant drawback of currently available hemostatic products is their susceptibility to bacterial infections at the bleeding site. As this is a prevalent issue that can potentially delay or compromise the healing process, there is an urgent demand for hemostatic agents with antibacterial properties to enhance survival rates. To mitigate the risk of infection at the site of a lesion, we propose an alternative solution in the form of a chitosan-based sponge and antimicrobial agents such as silver nanoparticles (AgNPs) and lavender essential oil (LEO). The aim of this work is to provide a new type of hemostatic sponge with an antibacterial barrier against a wide range of Gram-positive and Gram-negative microorganisms: Staphylococcus epidermidis 2018 and Enterococcus faecalis VRE 2566 (Gram-positive strains) and Klebsiella pneumoniae ATCC 10031 and Escherichia coli ATCC 35218 (Gram-negative strains).
Metal-Organic Cages Based on Phosphorescent Organometallics
Authors: Yu Yunliang , Wang Xiaoxia , Liu Yuliang , Zou Chao
Published: November 2023

During the last two decades, metal-organic cages (MOCs) have been extensively investigated and well documented. Meanwhile, phosphorescent MOCs have emerged as a kind of new MOC material but have not been given much attention. The diversity of their structures and their flexibility of self-assembly result in various luminescent behaviors. Additionally, their special photoactive properties are quite attractive in the background of photochemistry and worthy of discussion. Here, we would like to introduce the recent development of phosphorescent MOCs, including their structures, syntheses, photophysical properties and possible applications. This minireview may hopefully inspire the development of novel phosphorescent MOCs and also facilitate promising applications.
Bee Assemblage in the Southern Chihuahuan Desert: The Role of Season, Year, and Trap Color in Abundance
Authors: Munguia-Soto Esteban O. , Golubov Jordan , Mandujano María C.
Published: November 2023

Recognizing how populations fluctuate over time is a crucial factor in determining the environmental elements affecting population persistence. However, the limited information on wild bee populations complicates the estimation of the impact of anthropogenic threats leading to changes in population size. To address this, we conducted a study capturing and monitoring nine species of wild bees through monthly samplings over four years. Tray traps were placed in permanent plots, and capture records were used to determine population size (N) and density (D). A generalized linear model (GLM) was employed to determine how the use of traps affected bee species captures. The families Apidae and Halictidae represented the most captures. Apis mellifera, the Lasioglossum (Dialictus spp.) complex, and Macrotera sinaloana exhibited the largest number of captures and highest population density. Most species (77.7%) showed a tendency to remain constant over the years and to have a higher number of captures in the spring months. Moreover, yellow traps were the most effective in capturing bee individuals. We suggest that the availability of essential resources and the reduction in environmental stressors positively affected the capture of wild bee populations.
Insect-Mediated Pollination of Strawberries in an Urban Environment
Authors: Blareau Elsa , Sy Pauline , Daoud Karim , Requier Fabrice
Published: November 2023

Pollination services provided by a diversity of pollinators are critical in agriculture because they enhance the yield of many crops. However, few studies have assessed pollination services in urban agricultural systems. We performed flower–visitor observations and pollination experiments on strawberries (Fragaria × ananassa) in an urban area near Paris, France, in order to assess the effects of (i) insect-mediated pollination service and (ii) potential pollination deficit on fruit set, seed set, and fruit quality (size, weight, and malformation). Flower–visitor observations revealed that the pollinator community solely comprised unmanaged pollinators, despite the presence of beehives in the surrounding landscape. Based on the pollination experiments, we found that the pollination service mediated by wild insects improved the fruit size as a qualitative value of production, but not the fruit set. We also found no evidence of pollination deficit in our urban environment. These results suggest that the local community of wild urban pollinators is able to support strawberry crop production and thus plays an important role in providing high-quality, local, and sustainable crops in urban areas.
A Manifesto in Defense of Pain Complexity: A Critical Review of Essential Insights in Pain Neuroscience
Authors: Párraga Javier Picañol , Castellanos Aida
Published: November 2023

Chronic pain has increasingly become a significant health challenge, not just as a symptomatic manifestation but also as a pathological condition with profound socioeconomic implications. Despite the expansion of medical interventions, the prevalence of chronic pain remains remarkably persistent, prompting a turn towards non-pharmacological treatments, such as therapeutic education, exercise, and cognitive-behavioral therapy. With the advent of cognitive neuroscience, pain is often presented as a primary output derived from the brain, aligning with Engel’s Biopsychosocial Model that views disease not solely from a biological perspective but also considering psychological and social factors. This paradigm shift brings forward potential misconceptions and over-simplifications. The current review delves into the intricacies of nociception and pain perception. It questions long-standing beliefs like the cerebral-centric view of pain, the forgotten role of the peripheral nervous system in pain chronification, misconceptions around central sensitization syndromes, the controversy about the existence of a dedicated pain neuromatrix, the consciousness of the pain experience, and the possible oversight of factors beyond the nervous system. In re-evaluating these aspects, the review emphasizes the critical need for understanding the complexity of pain, urging the scientific and clinical community to move beyond reductionist perspectives and consider the multifaceted nature of this phenomenon.
The Impact of Rhythmic Physical Activity on Mental Health and Quality of Life in Older Adults with and without Cognitive Impairment: A Systematic Review and Meta-Analysis
Authors: Sánchez-Alcalá Marcelina , Aibar-Almazán Agustín , Afanador-Restrepo Diego Fernando , Carcelén-Fraile María del Carmen , Achalandabaso-Ochoa Alexander , Castellote-Caballero Yolanda , Hita-Contreras Fidel
Published: November 2023

(1) Background: Nowadays, it is essential to implement new non-pharmacological strategies, such as rhythmic physical activity, to improve mental health and quality of life in both individuals experiencing normal brain aging and those with cognitive impairment. Therefore, the objective of this study is to identify the effects of rhythmic physical activity interventions on mental health and quality of life in older adults, with or without mild cognitive impairment; (2) Methods: We conducted a systematic review with a meta-analysis, searching the Pubmed, Scopus, Web of Science, and Cochrane Plus databases using specific keywords. We selected studies that included rhythmic physical activity as the primary intervention for patients aged 65 and above, with or without cognitive impairment. We assessed the methodological quality of the articles using the PEDro scale; (3) Results: Out of 961 identified studies, we included 11 in this review, all of which employed rhythmic physical activity as an intervention. The selected studies consistently measured depression, anxiety, and quality of life; (4) Conclusions: This review demonstrates that rhythmic physical activity can effectively improve depression, anxiety, and quality of life in older adults, whether or not they have mild cognitive impairment. However, it is worth noting that while we have identified beneficial outcomes, the evidence supporting the use of rhythmic physical activity in enhancing depression, anxiety, and quality of life in older adults with or without mild cognitive impairment remains somewhat limited.
Can Dual Infrared–Visual Thermography Provide a More Reliable Diagnosis of Perforator Veins and Reflux Severity?
Authors: Dávalos Marcelo Pastor Almada , Brioschi Marcos Leal , da Rosa Samir Ezequiel , Brioschi Gabriel Carneiro , Neves Eduardo Borba
Published: November 2023

The accurate identification of perforator veins (PV) in asymptomatic veins that do not meet the criteria established by venous Doppler (VD) is a complex challenge, considered the gold standard in diagnosis, and is operator-dependent. This study explored the potential of dual infrared–visual thermography (IRVT) to identify PV in 99 patients aged 29 to 80 years. IRVT was conducted using a high-definition hyperspectral visual–infrared sensor. The temperature difference (ΔT) between maximum temperature (Tmax) and minimum temperature (Tmin) within the region of interest (ROI) served as an indicator for assessing vascular dysfunction severity. Comparative analysis was performed with VD results obtained using a Doppler ultrasound unit equipped with a 7.5 MHz linear transducer. Significant statistical differences (p < 0.05) in ΔT (Tmax−Tmin) were observed among PV sites categorized by reflux severity: no reflux (ΔT = 1.2 °C), mild reflux (ΔT = 1.8 °C), moderate reflux (ΔT = 2.9 °C), and severe reflux (ΔT = 3.6 °C). This study concludes that IRVT effectively distinguishes varying degrees of vascular reflux severity. IRVT shows promise as a non-invasive, radiation-free tool to enhance PV identification, especially in challenging cases, potentially improving patient outcomes and healthcare management. Further research is required to validate and refine its diagnostic utility.
Risk for Esophageal Cancer Based on Lifestyle Factors–Smoking, Alcohol Consumption, and Body Mass Index: Insight from a South Korean Population Study in a Low-Incidence Area
Authors: Kwon Mi Jung , Kang Ho Suk , Choi Hyo Geun , Kim Joo-Hee , Kim Ji Hee , Bang Woo Jin , Hong Sung Kwang , Kim Nan Young , Hong Sangkyoon , Lee Hong Kyu
Published: November 2023

Esophageal cancer constitutes a global public health challenge. However, South Korean population-specific information on the association of lifestyle (smoking, alcohol consumption, and obesity status) with esophageal cancer risk is sparse. This nested case–control study analyzed the Korean national health screening cohort data (2002–2019) of 1114 patients with esophageal cancer and 4456 controls (1:4 propensity-score matched for sex, age, income, and residential region). Conditional and unconditional logistic regression analyses, after adjustment for multiple covariates, determined the effects of lifestyle factors on esophageal cancer risk. Smoking and alcohol consumption increased the esophageal cancer risk (adjusted odds ratio [95% confidence interval]: 1.37 [1.15–1.63] and 1.89 [1.60–2.23], respectively). Overweight (body mass index [BMI] ≥ 23 to <25 kg/m2), obese I (BMI ≥ 25 to <30 kg/m2), or obese II (BMI ≥ 30 kg/m2) categories had reduced odds of esophageal cancer (0.76 [0.62–0.92], 0.59 [0.48–0.72], and 0.47 [0.26–0.85], respectively). In the subgroup analyses, the association of incident esophageal cancer with smoking and alcohol consumption persisted, particularly in men or those aged ≥55 years, whereas higher BMI scores remained consistently associated with a reduced esophageal cancer likelihood across all age groups, in both sexes, and alcohol users or current smokers. Underweight current smokers exhibited a higher propensity for esophageal cancer. In conclusion, smoking and alcohol drinking may potentially increase the risk, whereas weight maintenance, with BMI ≥ 23 kg/m2, may potentially decrease the risk, for esophageal cancer in the South Korean population. Lifestyle modification in the specific subgroups may be a potential strategy for preventing esophageal cancer.
The Evaluation of the Balance Test and MuscleLab Test for the Early Detection of Femoroacetabular Impingement
Authors: Centemeri Roberto , Riva Michele Augusto , Belingheri Michael , Paladino Maria Emilia , D’Orso Marco Italo , Intra Jari
Published: November 2023

Femoroacetabular impingement (FAI) is a common source of hip pain affecting a wide range of subjects. In this work, we assessed two tests, namely the balance test and the MuscleLab test, in comparison with the flexion–abduction–external rotation (FABER) and flexion–adduction–internal rotation (FADIR) tests, in order to evaluate the functionality of the neural–musculoskeletal system of the subjects affected by FAI based on the measurement of biomechanical parameters. Our goal was to investigate the early detection of an altered hip joint and to monitor pathology progression over time. A total of 52 subjects, 29 females and 23 males, with an average age of 42 ± 13 years presenting hip impingement diagnosed using X-ray, were enrolled. Twenty-eight patients without signs of hip impingements were used as the control group. The balance test, which evaluates the capacity of a person to keep the orthostatic position against terrestrial gravity, and the MuscleLab test, which measures the force and power generated by active muscles during a movement, as well as FABER and FADIR tests, were performed in each subject. The balance and MuscleLab tests presented 100% sensitivity and higher sensitivity in FAI diagnosis, with 72.9% and 70.4%, respectively, in comparison with those obtained using FABER and FADIR tests, with 59.6% and 67.3%, respectively. The evaluation of the neural–musculoskeletal system using the balance and MuscleLab tests can help in the early detection of the severity of hip impingements and the assessment of non-operative treatments used over time.
Modification of Recycled Concrete Aggregate and Its Use in Concrete: An Overview of Research Progress
Authors: Su Yingqiang , Yao Yuchong , Wang Yang , Zhao Xuan , Li Li , Zhang Jie
Published: November 2023

The differences in physical properties, chemical properties, and mechanical properties between reclaimed concrete aggregate and natural aggregate are discussed in this paper. In this paper, the commonly used improvement techniques of recycled concrete aggregate are reviewed. Physical modification involves peeling the attached mortar layer using mechanical and thermodynamic means, including mechanical grinding and shaping, heat treatment, and microwave or electric pulse treatment. Chemical modification is based on the chemical reaction of some materials with recycled aggregate attached mortar, including acid treatment removal, water glass strengthening, carbonation strengthening, inorganic slurry strengthening, and polymer strengthening. Microbial modification is mainly based on the metabolic activity of specific microorganisms that induce carbon deposition modification. The results show that the reinforced technology of recycled aggregate has made some progress in improving the performance of recycled aggregate, but there are still some problems, such as inconsistent strengthening effects and the unstable compatibility of composite materials. In this paper, future research directions, such as the development of new strengthening materials and the integration of multi-functional strengthening technology, are described in order to provide some theoretical support for the utilization of recycled concrete aggregate.
Differences in Performance and Conductivity Persistence of New Reduced Graphene Oxide Air Filter Materials before and after Eliminating Static Electricity
Authors: Gao Yun , Shi Huixin , Zhang Xin , Ma Jingyao , Yu Tao
Published: November 2023

Improving the filtration efficiency of air filter materials is an ongoing research goal. This study conducted in-depth research on a new reduced graphene oxide air filter material, and the differences in its performance and conductivity durability before and after eliminating static electricity were tested and analyzed. The results showed that the filtration efficiency of the reduced graphene oxide air filter material significantly decreased after eliminating static electricity. The maximum decrease in filtration efficiency was observed at a filtration velocity of 0.8 m/s, with PM10 > PM1.0 > PM2.5. In this case, the filtration efficiency decreased by 11.8%, 7.98%, and 7.17%, respectively. The maximum difference in filtration efficiency of 0.29 μm particulates was about 12.7%. Eliminating static electricity slightly increased the resistance (2.5~15.5 Pa). In addition, the new reduced graphene oxide air filter material exhibited good conductivity and stability after continuous testing. This study provides data support for the application of subsequent electrification sterilization, reference values for multi-angle applications, and the development of new composite air filter materials.
Characterization of Densified Pine Wood and a Zero-Thickness Bio-Based Adhesive for Eco-Friendly Structural Applications
Authors: Jalali Shahin , Borges Catarina da Silva Pereira , Carbas Ricardo João Camilo , Marques Eduardo André de Sousa , Bordado João Carlos Moura , da Silva Lucas Filipe Martins
Published: November 2023

This study investigates a sustainable alternative for composites and adhesives in high-performance industries like civil and automotive. This study pioneers the development and application of a new methodology to characterize a bio-based, zero-thickness adhesive. This method facilitates precise measurements of the adhesive’s strength and fracture properties under zero-thickness conditions. The research also encompasses the characterization of densified pine wood, an innovative wood product distinguished by enhanced mechanical properties, which is subsequently compared to natural pine wood. We conducted a comprehensive characterization of wood’s strength properties, utilizing dogbone-shaped samples in the fiber direction, and block specimens in the transverse direction. Butt joints were employed for adhesive testing. Mode I fracture properties were determined via compact tension (CT) and double cantilever beam (DCB) tests for wood and adhesive, respectively, while mode II response was assessed through end-loaded split (ELS) tests. The densification procedure, encompassing chemical and mechanical processes, was a focal point of the study. Initially, wood was subjected to acid boiling to remove the wood matrix, followed by the application of pressure to enhance density. As a result, wood density increased by approximately 100 percent, accompanied by substantial improvements in strength and fracture energy along the fiber direction by about 120 percent. However, it is worth noting that due to the delignification nature of the densification method, properties in the transverse direction, mainly reliant on the lignin matrix, exhibited compromises. Also introduced was an innovative technique to evaluate the bio-based adhesive, applied as a zero-thickness layer. The results from this method reveal promising mechanical properties, highlighting the bio-based adhesive’s potential as an eco-friendly substitute for synthetic adhesives in the wood industry.
Failure Analysis of Cracked P110 Repaired Tubing Used for Gas Transmission
Authors: Zhang Shuxin , Xie Faqin , Wu Xiangqing , Yan Xi , Luo Jinheng , Ma Xiaoliang , Su Gege
Published: November 2023

With green and low-carbon developments in oil fields, an increasing amount of repaired oil tubing is being used as oil and gas transmission pipelines in China. However, due to differences in manufacturing standards between oil tubing and transmission pipelines, there are inevitably some issues during their use. This paper investigates a case of cracking failure in repaired oil tubing used as a gathering and transportation pipeline. The failure occurred after eight months of operation and was characterized by a circumferential crack at the male thread end of the tubing joint. To determine the root cause of the failure, a series of experiments were conducted on the oil tubing. The experiments included visual inspection, chemical composition analysis, mechanical properties testing, hardness testing, metallographic examination, and microstructure analysis. The results revealed that the thread of the cracked tubing was not tightened to the specified position; the connection between the tubing and the coupling was welded in a circumferential direction; and cracks occurred in the heat-affected zone of the weld. Chemical composition, tensile performance, and the Charpy impact of the tubing meet the requirements of API 5CT for P110 material, and no abnormalities were found in the metallographic structure. The microstructure at the weld toe of the fracture is martensite, and the hardness is 476 HV10. Based on the thermal simulation verification test, when the material of the tubing cools from 1200 °C, which is located in the coarse HAZ temperature zone, the base metal transforms into martensite with a little granular bainite, exhibiting its highest hardness value at 371 HV10, which is higher than the allowable hardness for carbon steel and indicates the material has poor weldability. The reasons for the cracking and failure of the tubing are that the P110 repaired tubing has a high carbon equivalent and poor weldability. During the welding process, martensitic structure was formed at the weld toe, and cold cracks appeared in the heat-affected zone, resulting in failure. To avoid the reoccurrence of such failure, recommendations are proposed.
The Bending Properties of Hybrid Cross-Laminated Timber (CLT) Using Various Species Combinations
Authors: Altaher Omer Ahmed Ahmed , Garab József , Horváth-Szováti Erika , Kozelka János , Bejó László
Published: November 2023

Cross-laminated timber (CLT) has become a massive commercial success in recent years due to its high performance, technological advantages, and low environmental impact. The finite softwood raw material supply has motivated researchers to find alternatives. This study presents an investigation of the viability of some Hungarian hardwood materials, such as CLT materials. Homogeneous beech, poplar, and spruce panels, as well as their combinations, were created using a polyurethane adhesive. The experimental results show the clear potential of Hungarian poplar, which performed much better than spruce. Poplar’s modulus of elasticity (MOE) and modulus of rupture (MOR) values reached or exceeded those of high-grade commercial softwood CLT. The bending properties of beech and hybrid beech–poplar panels far exceeded the performance of commercial panels, which shows the excellent potential of high-density hardwoods for high-performance CLT production. Beech–spruce hybrid panels seriously underperformed. This was caused by gluing issues, probably due to the large density differences between the two species, as evidenced by the glueline failure exhibited by most of these specimens during testing. The average panel density proved to be the best predictor of mechanical performance, except for beech–spruce hybrid panels.
Largely Enhanced Thermoelectric Power Factor of Flexible Cu2−xS Film by Doping Mn
Authors: Zuo Xinru , Han Xiaowen , Lu Yiming , Liu Ying , Wang Zixing , Li Jiajia , Cai Kefeng
Published: November 2023

Copper-sulfide-based materials have attracted noteworthy attention as thermoelectric materials due to rich elemental reserves, non-toxicity, low thermal conductivity, and adjustable electrical properties. However, research on the flexible thermoelectrics of copper sulfide has not yet been reported. In this work, we developed a facile method to prepare flexible Mn-doped Cu2−xS films on nylon membranes. First, nano to submicron powders with nominal compositions of Cu2−xMnyS (y = 0, 0.01, 0.03, 0.05, 0.07) were synthesized by a hydrothermal method. Then, the powders were vacuum-filtrated on nylon membranes and finally hot-pressed. Phase composition and microstructure analysis revealed that the films contained both Cu2S and Cu1.96S, and the size of the grains was ~20–300 nm. By Mn doping, there was an increase in carrier concentration and mobility, and ultimately, the electrical properties of Cu2−xS were improved. Eventually, the Cu2−xMn0.05S film showed a maximum power factor of 113.3 μW m−1 K−2 and good flexibility at room temperature. Moreover, an assembled four-leg flexible thermoelectric generator produced a maximum power of 249.48 nW (corresponding power density ~1.23 W m−2) at a temperature difference of 30.1 K, and had good potential for powering low-power-consumption wearable electronics.
Quantitative Characterization of Elemental Segregation in Inconel 718 Superalloy by Micro-Beam X-ray Fluorescence Spectroscopy and Its Correlation Study
Authors: Zhou Xuefan , Li Dongling , Zhou Qingqing , Jiang Fan , Song Yan , Liang Wanying , Liu Mingbo , Shen Xuejing , Wang Haizhou
Published: November 2023

Inconel 718 (IN718) nickel-based superalloy is widely used in aerospace and nuclear applications owing to its excellent comprehensive mechanical properties, oxidation resistance, and hot corrosion resistance. However, the elemental segregation caused by heterogeneous solidification during casting has great influence on the mechanical properties. Therefore, accurately characterizing the segregation behavior is necessary. Traditional quantitative characterization of elemental segregation uses various sampling methods, in which only macroscopic segregation results are obtained. In this study, micro-beam X-ray fluorescence (μ-XRF) is used for the quantitative characterization of element micro-segregation in IN718 superalloy. The concentration distributions of Cr, Fe, Mo, Nb, and Ti in IN718 alloy are determined with optimized testing parameters, and the degree of elemental segregation in different regions of the analytical area is calculated. It is found that the segregation degree of Nb and Ti in the testing area is larger than other alloying elements. The correlation between the microstructure distribution and the segregation degree of Nb and Ti has been studied using scanning electron microscopy (SEM) combined with energy-dispersive spectrometry (EDS). There is severe segregation of Nb and Ti in areas where Nb-containing precipitates are accumulated. The distribution of abnormal signals of Nb with a high fluorescence intensity has a close relationship with the area of precipitates-enriched Nb.
Mouse N2a Neuroblastoma Assay: Uncertainties and Comparison with Alternative Cell-Based Assays for Ciguatoxin Detection
Authors: Raposo-Garcia Sandra , Cao Alejandro , Costas Celia , Louzao M. Carmen , Vilariño Natalia , Vale Carmen , Botana Luis M.
Published: November 2023

The growing concern about ciguatera fish poisoning (CF) due to the expansion of the microorganisms producing ciguatoxins (CTXs) increased the need to develop a reliable and fast method for ciguatoxin detection to guarantee food safety. Cytotoxicity assay on the N2a cells sensitized with ouabain (O) and veratridine (V) is routinely used in ciguatoxin detection; however, this method has not been standardized yet. This study demonstrated the low availability of sodium channels in the N2a cells, the great O/V damage to the cells and the cell detachment when the cell viability is evaluated by the classical cytotoxicity assay and confirmed the absence of toxic effects caused by CTXs alone when using the methods that do not require medium removal such as lactate dehydrogenase (LDH) and Alamar blue assays. Different cell lines were evaluated as alternatives, such as human neuroblastoma, which was not suitable for the CTX detection due to the greater sensitivity to O/V and low availability of sodium channels. However, the HEK293 Nav cell line expressing the α1.6 subunit of sodium channels was sensitive to the ciguatoxin without the sensitization with O/V due to its expression of sodium channels. In the case of sensitizing the cells with O/V, it was possible to detect the presence of the ciguatoxin by the classical cytotoxicity MTT method at concentrations as low as 0.0001 nM CTX3C, providing an alternative cell line for the detection of compounds that act on the sodium channels.
Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats
Authors: Koshak Maimonah Fuad , El-Readi Mahmoud Zaki , Elzubier Mohamed Elzubier , Refaat Bassem , Almaimani Riyad Adnan , Idris Shakir , Althubiti Mohammad , Al-Amodi Hiba Saeed , Eid Safaa Yehia
Published: November 2023

Paracetamol or acetaminophen (PAC) is a commonly used analgesic and antipyretic drug. It has been shown that overdoses beyond the therapeutic range can cause hepatotoxicity and acute liver injury. The most common cause of drug-induced liver injury (DILI) in Saudi Arabia and worldwide is paracetamol overdose. Fucoxanthin (FUC) is an allenic carotenoid that is found in edible brown seaweeds, and it has antioxidant and anti-inflammatory effects. Several studies have shown the potential therapeutic effects of FUC in diabetes, cancers, and inflammatory disorders. This study aims to investigate the protective effect of FUC against PAC-induced acute liver injury in rats. FUC was administered (100, 200, and 500 mg/kg, p.o.) for 7 days, and then the liver injury was induced by the administration of PAC (2000 mg/kg, oral). Blood and liver tissue samples were collected from PAC-positive untreated, treated, and negative control rats. Biochemical and inflammatory parameters in the blood were measured. In addition, RT-PCR, Western blotting, and immunohistochemistry were performed for liver tissue. The serum levels of liver biomarkers (ALT, AST, and ALP) increased after PAC-induced liver toxicity; FUC-treated rats showed lower levels compared to the positive control. There was an increase in the expression of TNF-α, IL-1, IL-6, NF-kB, INF-γ, and iNOS and a decrease in IL-10, IL-22, and IL-10R expression after the FUC treatment of injured liver rats. For the hepatic inflammation and PAC-toxicity-induced oxidative stress genes and proteins, FUC-treated rats (100, 200, and 500 mg/kg) showed a reduction in the expression of oxidative stress genes. These results showed that FUC protected the liver against PAC-induced injury through antioxidant and anti-inflammatory actions. However, further clinical studies are required to confirm the findings.
Correlation between Preoperative MRI Parameters and Oswestry Disability Index in Patients with Lumbar Spinal Stenosis: A Retrospective Study
Authors: Caprariu Radu , Oprea Manuel D. , Poenaru Dan V. , Andrei Diana
Published: November 2023

Background and Objectives: Lumbar spinal stenosis (LSS) is a degenerative condition posing significant challenges in clinical management. Despite the use of radiological parameters and patient-reported outcome measures like the Oswestry Disability Index (ODI) for evaluation, there is limited understanding of their interrelationship. This study aimed to investigate the correlation between preoperative MRI parameters and ODI scores in patients with LSS undergoing surgical treatment. Materials and Methods: A retrospective analysis was conducted on 86 patients diagnosed with LSS over a 5-year period. Preoperative MRI measurements, including the cross-sectional area of the psoas muscle, lumbar canal stenosis, neural foramina area, and facet joint osteoarthritis, were assessed. ODI scores were collected preoperatively and at a 1-year follow-up. Statistical analyses were performed using IBM SPSS Statistics software (version 26). Results: Weak to moderate correlations were observed between certain MRI parameters and ODI scores. The initial ODI score had a weak positive correlation with the severity of lumbar canal stenosis according to Schizas criteria (rho = 0.327, p = 0.010) and a moderate negative correlation with the relative cross-sectional area of the psoas muscle (rho = −0.498, p = 0.000). At 1-year follow-up, the ODI had a weak negative correlation with the relative cross-sectional area of the psoas muscle (rho = −0.284, p = 0.026). Conclusions: While the severity of LSS showed a weak correlation with initial ODI, it was not a predictor of 1-year postoperative ODI. Furthermore, although the cross-sectional area of the thecal sac, the sagittal area of the neural foramen, and the grade of facet joint osteoarthritis influence the imagistic severity, none of them correlate with ODI. These findings underscore the need for a comprehensive model that integrates multiple imaging and clinical parameters for a holistic understanding of LSS and its functional outcomes.
Selective Laser Melting of Non-Weldable Nickel Superalloy: Microstructure, Cracks and Texture
Authors: Starikov Kirill , Polozov Igor , Borisov Evgenii , Kim Artem , Voevodenko Daniil , Gracheva Anna , Shamshurin Alexey , Popovich Anatoly
Published: November 2023

Additive manufacturing, particularly selective laser melting, presents exciting possibilities for fabricating components from high-temperature nickel-based superalloys. Controlling microstructural features and minimizing defects in fabricated specimens are critical challenges. This study explores the influence of process parameters on microstructure and defect formation in directionally solidified nickel-based superalloy specimens. We conducted a comprehensive analysis of selective laser melting process variables, including interdendritic spacing, crystallization times, and volumetric energy density. Electron backscatter diffraction analysis was employed to assess the feasibility of obtaining a directional structure in single-crystal nickel-based heat-resistant alloy specimens using selective laser melting. The study shows a significant correlation between reduced interdendritic spacing and increased defect formation. Longer crystallization times and higher volumetric energy density lead to decreased defect volumes and sizes. Electron backscatter diffraction analysis confirms the maintenance of preferential growth direction across subsequent layers. Our research underscores the importance of optimizing selective laser melting parameters, balancing refractory elements in alloy composition, and adopting strategies for enhancing crystallization times to minimize structural defects. This comprehensive approach ensures both heat resistance and minimal defects, facilitating the production of high-quality components. These findings contribute to advancing selective laser melting applications in critical industries like aerospace and power generation, where heat-resistant materials are paramount.