bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Somatostatin Receptor Subtype 1 (SSTR1) Gene Polymorphism and Their Association with Growth Traits in Hulun Buir Sheep
Authors: Li Xue , Ding Ning , Zhang Zhichao , Tian Dehong , Han Buying , Liu Sijia , Liu Dehui , Tian Fei , Zhao Kai
Published: December 2021

This study was conducted to evaluate SSTR1 gene polymorphisms and their association with growth traits in Hulun Buir sheep. We followed 233 Hulun Buir sheep from birth to 16 months of age, born in the same pasture and on the same year under a consistent grazing conditions. The body weight (BW), body height (BH), body length (BL), chest circumference (ChC), chest depth (ChD), chest width (ChW), hip width (HW), and cannon circumference (CaC) were measured and recorded at birth, 4 months, 9 months, and 16 months of age. The polymorphisms of the SSTR1 gene in Hulun Buir sheep were excavated using exon sequencing, and association analyses of between SNPs and growth traits at each growth stage were conducted. The results showed that there were four SNPs in Exon 2 of the SSTR1 gene, SNP1, SNP2, and SNP3 were low mutation sites, and SNP4 was a moderate mutation site. Four SNPs were consistent with Hardy–Weinberg equilibrium, and all of them were synonymous mutations. The association analyses found that the genotypes of SNP2 were significantly associated with WW and BH at 4 months of age, BW, BL, ChC, and HW at 9 months of age (p < 0.05), and extremely significantly associated with ChD at 4 and 9 months of age (p < 0.01). There were significant associations between SNP3 and BH at 9 months of age, between SNP4 and ChD, ChW, and CaC at 9 months of age, and BW and ChC at 16 months of age (p < 0.05). There were no detectable associations with growth traits among the seven haplotypes between the SNP1, 3, and 4 of a strong linkage disequilibrium (p > 0.05). These results indicated that SNP2, SNP3, and SNP4 may be used as molecular markers for growth traits of Hulun Buir sheep.
Identification of Compound Heterozygous Variants in LRP4 Demonstrates That a Pathogenic Variant outside the Third β-Propeller Domain Can Cause Sclerosteosis
Authors: Huybrechts Yentl , Boudin Eveline , Hendrickx Gretl , Steenackers Ellen , Hamdy Neveen , Mortier Geert , Martínez Díaz-Guerra Guillermo , Bracamonte Milagros Sierra , Appelman-Dijkstra Natasha M. , Van Hul Wim
Published: December 2021

Sclerosteosis is a high bone mass disorder, caused by pathogenic variants in the genes encoding sclerostin or LRP4. Both proteins form a complex that strongly inhibits canonical WNT signaling activity, a pathway of major importance in bone formation. So far, all reported disease-causing variants are located in the third β-propeller domain of LRP4, which is essential for the interaction with sclerostin. Here, we report the identification of two compound heterozygous variants, a known p.Arg1170Gln and a novel p.Arg632His variant, in a patient with a sclerosteosis phenotype. Interestingly, the novel variant is located in the first β-propeller domain, which is known to be indispensable for the interaction with agrin. However, using luciferase reporter assays, we demonstrated that both the p.Arg1170Gln and the p.Arg632His variant in LRP4 reduced the inhibitory capacity of sclerostin on canonical WNT signaling activity. In conclusion, this study is the first to demonstrate that a pathogenic variant in the first β-propeller domain of LRP4 can contribute to the development of sclerosteosis, which broadens the mutational spectrum of the disorder.
Effect of miR-302b MicroRNA Inhibition on Chicken Primordial Germ Cell Proliferation and Apoptosis Rate
Authors: Lázár Bence , Szabadi Nikolett Tokodyné , Anand Mahek , Tóth Roland , Ecker András , Urbán Martin , Aponte Maria Teresa Salinas , Stepanova Ganna , Hegyi Zoltán , Homolya László , Várkonyi Eszter Patakiné , Pain Bertrand , Gócza Elen
Published: December 2021

The primordial germ cells (PGCs) are the precursors for both the oocytes and spermatogonia. Recently, a novel culture system was established for chicken PGCs, isolated from embryonic blood. The possibility of PGC long-term cultivation issues a new advance in germ cell preservation, biotechnology, and cell biology. We investigated the consequence of gga-miR-302b-5P (5P), gga-miR-302b-3P (3P) and dual inhibition (5P/3P) in two male and two female chicken PGC lines. In treated and control cell cultures, the cell number was calculated every four hours for three days by the XLS Imaging system. Comparing the cell number of control and treated lines on the first day, we found that male lines had a higher proliferation rate independently from the treatments. Compared to the untreated ones, the proliferation rate and the number of apoptotic cells were considerably reduced at gga-miR-302b-5P inhibition in all PGC lines on the third day of the cultivation. The control PGC lines showed a significantly higher proliferation rate than 3P inhibited lines on Day 3 in all PGC lines. Dual inhibition of gga-miR-302b mature miRNAs caused a slight reduction in proliferation rate, but the number of apoptotic cells increased dramatically. The information gathered by examining the factors affecting cell proliferation of PGCs can lead to new data in stem cell biology.
Complement-Mediated Selective Tumor Cell Lysis Enabled by Bi-Functional RNA Aptamers
Authors: Mallik Prabhat K. , Nishikawa Kimi , Mallik Pramit , Shi Hua
Published: December 2021

Unlike microbes that infect the human body, cancer cells are descended from normal cells and are not easily recognizable as “foreign” by the immune system of the host. However, if the malignant cells can be specifically earmarked for attack by a synthetic “designator”, the powerful effector mechanisms of the immune response can be conscripted to treat cancer. To implement this strategy, we have been developing aptamer-derived molecular adaptors to invoke synthetic immune responses against cancer cells. Here we describe multi-valent aptamers that simultaneously bind target molecules on the surface of cancer cells and an activated complement protein, which would tag the target molecules and their associated cells as “foreign” and trigger multiple effector mechanisms. Increased deposition of the complement proteins on the surface of cancer cells via aptamer binding to membrane targets could induce the formation of the membrane attack complex or cytotoxic degranulation by phagocytes and natural killer cells, thereby causing irreversible destruction of the targeted cells. Specifically, we designed and constructed a bi-functional aptamer linking EGFR and C3b/iC3b, and used it in a cell-based assay to cause lysis of MDA-MB-231 and BT-20 breast cancer cells, with either human or mouse serum as the source of complement factors.
Capturing SNP Association across the NK Receptor and HLA Gene Regions in Multiple Sclerosis by Targeted Penalised Regression Models
Authors: Burnard Sean M. , Lea Rodney A. , Benton Miles , Eccles David , Kennedy Daniel W. , Lechner-Scott Jeannette , Scott Rodney J.
Published: December 2021

Conventional genome-wide association studies (GWASs) of complex traits, such as Multiple Sclerosis (MS), are reliant on per-SNP p-values and are therefore heavily burdened by multiple testing correction. Thus, in order to detect more subtle alterations, ever increasing sample sizes are required, while ignoring potentially valuable information that is readily available in existing datasets. To overcome this, we used penalised regression incorporating elastic net with a stability selection method by iterative subsampling to detect the potential interaction of loci with MS risk. Through re-analysis of the ANZgene dataset (1617 cases and 1988 controls) and an IMSGC dataset as a replication cohort (1313 cases and 1458 controls), we identified new association signals for MS predisposition, including SNPs above and below conventional significance thresholds while targeting two natural killer receptor loci and the well-established HLA loci. For example, rs2844482 (98.1% iterations), otherwise ignored by conventional statistics (p = 0.673) in the same dataset, was independently strongly associated with MS in another GWAS that required more than 40 times the number of cases (~45 K). Further comparison of our hits to those present in a large-scale meta-analysis, confirmed that the majority of SNPs identified by the elastic net model reached conventional statistical GWAS thresholds (p < 5 × 10−8) in this much larger dataset. Moreover, we found that gene variants involved in oxidative stress, in addition to innate immunity, were associated with MS. Overall, this study highlights the benefit of using more advanced statistical methods to (re-)analyse subtle genetic variation among loci that have a biological basis for their contribution to disease risk.
Comprehensive Analysis of Innate Immunophenotyping Based on Immune Score Predicting Immune Alterations and Prognosis in Breast Cancer Patients
Authors: Liu Weiguang , Xia Lingling , Xia Zhengmiao , Chen Liming
Published: December 2021

Breast cancer is the most common cancer, with the highest mortality rate and the most diagnosed cancer type in women worldwide. To identify the effect innate immune checkpoint for breast cancer immunotherapy, the innate immune prognostic biomarkers were selected through the ICI score model and the risk model in breast cancer patients. Moreover, the reliability and accuracy of the ICI score model and the risk model were further examined through the analysis of breast cancer prognosis and immune cell infiltration. The pan cancer analysis further confirmed and selected CXCL9 as the key innate immune checkpoint for breast cancer immunotherapy and identified three small molecular drugs for target CXCL9 through molecular docking analysis. In summary, CXCL9 significantly correlated with the prognostic of breast cancer and immune cell infiltration and could be innate immune checkpoint for breast cancer immunotherapy.
Understanding Transcriptomic and Serological Differences between Forced Molting and Natural Molting in Laying Hens
Authors: Zhang Tongyu , Ning Zhonghua , Chen Yu , Wen Junhui , Jia Yaxiong , Wang Liang , Lv Xueze , Yang Weifang , Qu Changqing , Li Haiying , Wang Huie , Qu Lujiang
Published: December 2021

Molting is natural adaptation to climate change in all birds, including chickens. Forced molting (FM) can rejuvenate and reactivate the reproductive potential of aged hens, but the effect of natural molting (NM) on older chickens is not clear. To explore why FM has a dramatically different effect on chickens compared with NM, the transcriptome analyses of the hypothalamus and ovary in forced molted and natural molted hens at two periods with feathers fallen and regrown were performed. Additionally, each experimental chicken was tested for serological indices. The results of serological indices showed that growth hormone, thyroid stimulating hormone, and thyroxine levels were significantly higher (p < 0.05) in forced molted hens than in natural molted hens, and calcitonin concentrations were lower in the forced molted than in the natural molted hens. Furthermore, the transcriptomic analysis revealed a large number of genes related to disease resistance and anti-aging in the two different FM and NM periods. These regulatory genes and serological indices promote reproductive function during FM. This study systematically revealed the transcriptomic and serological differences between FM and NM, which could broaden our understanding of aging, rejuvenation, egg production, and welfare issues related to FM in chickens.
A Predictable Approach of a Rare and Frequently Misdiagnosed Entity: Laryngeal Nerve Schwannoma
Authors: Filipov Iulian , Chirila Lucian , Sandulescu Mihai , Cristache Corina Marilena
Published: December 2021

(1) Background: Schwannoma, a mesenchymal neoplasm derived from Schwann cells that line peripheral nerve sheaths, has a challenging diagnosis, due to the non-specific medical history and clinical examination. Nowadays, virtual reality (VR) is increasingly more used for enhancing diagnosis and for preoperative planning of surgical procedures. With VR, the surgeon can interact, before any surgery, with a virtual environment that is completely generated by a computer, offering them a real experience inside a virtual 3D model. (2) Methods and Results: The aim of the present paper was to present a case of surgically removal of a schwannoma, which originated from the fibers of the superior laryngeal nerve, in a predictable and minimally invasive fashion, upon using VR for diagnosis and surgical procedure planning. (3) Conclusions: The current clinical report attracted the attention of including schwannoma in the possible differential diagnosis of a swelling in the anterior cervical region, mainly when a nonspecific radiological appearance is noticed, even with the use of multiple imaging modalities. Virtual reality can increase the predictability and success rate of the surgical procedure, being in the meantime a good tool for communication with the patient.
The Role of Methyl Donors of the Methionine Cycle in Gastrointestinal Infection and Inflammation
Authors: Vaccaro Joseph A. , Naser Saleh A.
Published: December 2021

Vitamin deficiency is well known to contribute to disease development in both humans and other animals. Nonetheless, truly understanding the role of vitamins in human biology requires more than identifying their deficiencies. Discerning the mechanisms by which vitamins participate in health is necessary to assess risk factors, diagnostics, and treatment options for deficiency in a clinical setting. For researchers, the absence of a vitamin may be used as a tool to understand the importance of the metabolic pathways in which it participates. This review aims to explore the current understanding of the complex relationship between the methyl donating vitamins folate and cobalamin (B12), the universal methyl donor S-adenosyl-L-methionine (SAM), and inflammatory processes in human disease. First, it outlines the process of single-carbon metabolism in the generation of first methionine and subsequently SAM. Following this, established relationships between folate, B12, and SAM in varying bodily tissues are discussed, with special attention given to their effects on gut inflammation.
Analysis of Growth and Yield of Three Types Cucumbers (Cucumis sativus L.) Based on Yield Components
Authors: Maeda Kazuya , Ahn Dong-Hyuk
Published: December 2021

The objectives of this study were to evaluate the yield of different types of cucumbers from the perspective of yield components. The yield and yield components of nine cucumber cultivars (Beit Alpha, Greenhouse, and Japanese) grown hydroponically in a greenhouse were investigated. Fruit yield fresh weight in the Beit Alpha type was higher than that of the Japanese type, and the fruit yield fresh weight was significantly correlated with fruit yield dry weight, total dry matter (TDM), fraction of fruits, and number of fruits. However, the fruit yield fresh weight was not significantly correlated with intercepted light, light use efficiency (LUE), leaf area index, light extinction coefficient, and fruit dry matter content. High fruit yield dry weight was associated with a high TDM and the fraction of fruits. Moreover, the high TDM and fraction of fruits were associated with LUE and the number of fruits, respectively.
Pseudocochlodinium profundisulcus Resting Cysts Detected in the Ballast Tank Sediment of Ships Arriving in the Ports of China and North America and the Implications in the Species’ Geographic Distribution and Possible Invasion
Authors: Shang Lixia , Zhai Xinyu , Tian Wen , Liu Yuyang , Han Yangchun , Deng Yunyan , Hu Zhangxi , Tang Ying Zhong
Published: December 2021

Over the past several decades, much attention has been focused on the dispersal of aquatic nonindigenous species via ballast tanks of shipping vessels worldwide. The recently reclassified dinoflagellate Pseudocochlodinium profundisulcus (previously identified as Cochlodinium sp., Cochlodinium geminatum, or Polykrikos geminatus) was not reported in China until 2006. However, algal blooming events caused by this organism have been reported almost every year since then in the Pearl River Estuary and its adjacent areas in China. Whether P. profundisulcus is an indigenous or an invasive species has thus become an ecological question of great scientific and practical significance. In this study, we collected the sediments from ballast tanks of ships arriving in the ports of China and North America and characterized dinoflagellate resting cysts via a combined approach. We germinated two dark brownish cysts from the tank of an international ship (Vessel A) arriving at the Jiangyin Port (China) into vegetative cells and identified them as P. profundisulcus by light and scanning electron microscopy and phylogenetic analyses for partial LSU rDNA sequences. We also identified P. profundisulcus cyst from the ballast tank sediment of a ship (Vessel B) arriving in the port of North America via single-cyst PCR and cloning sequencing, which indicated that this species could be transported as resting cyst via ship. Since phylogenetic analyses based on partial LSU rDNA sequences could not differentiate all sequences among our cysts from those deposited in the NCBI database into sub-groups, all populations from China, Australia, Japan, and the original sources from which the cysts in the two vessels arrived in China and North America were carried over appeared to share a very recent common ancestor, and the species may have experienced a worldwide expansion recently. These results indicate that P. profundisulcus cysts may have been extensively transferred to many regions of the world via ships’ ballast tank sediments. While our work provides an exemplary case for both the feasibility and complexity (in tracking the source) of the bio-invasion risk via the transport of live resting cysts by ship’s ballast tanks, it also points out an orientation for future investigation.
Stress Perception, Sleep Quality and Work Engagement of German Outpatient Nurses during the COVID-19 Pandemic
Authors: Bernburg Monika , Hetzmann Mara Shirin , Mojtahedzadeh Natascha , Neumann Felix Alexander , Augustin Matthias , Harth Volker , Groneberg David Alexander , Zyriax Birgit-Christiane , Mache Stefanie
Published: December 2021

Since the outbreak of the COVID-19 pandemic, outpatient nurses have been exposed to a double burden of already known occupational and new pandemic-related stressors. Recent studies suggest that increased pandemic-related stress can affect mental health and promote the development of negative mental health outcomes for nurses. This includes a decrease in sleep quality and work engagement. In addition, certain groups appear to be particularly vulnerable to pandemic-related stress. The aim of this study was to investigate the stress perception of German outpatient nurses during the COVID-19 pandemic. The aim was to determine associations between their pandemic-related stress and variables such as sleep quality, work engagement, pandemic-related worries and concerns. For this purpose, a questionnaire was developed based on well-established measurement instruments such as the 10-item Perceived Stress Scale, the Pittsburgh Sleep Quality Index and the Copenhagen Psychosocial Questionnaire to conduct a cross-sectional online survey among outpatient nurses from Germany. Participants (n = 166) showed rather moderate overall pandemic-related stress levels, good sleep quality, high work engagement, and moderate pandemic-related worries and concerns. Pandemic-related stress proved to be a predictor of decreased sleep quality and work engagement of outpatient nurses with weak effect sizes. Despite the surprisingly moderate stress levels, the effects of pandemic-related stress on selected aspects of participants’ mental health could be demonstrated. Therefore, behavioural and organisational health promotion measures are recommended to support outpatient nurses during the pandemic. However, further research is needed to determine the causal relationships and long-term effects of pandemic-related stress on the mental health of outpatient caregivers.
Sex-Related Predisposition to Post-Traumatic Stress Disorder Development—The Role of Neuropeptides
Authors: Lehner Małgorzata , Skórzewska Anna , Wisłowska-Stanek Aleksandra
Published: December 2021

Post-traumatic stress disorder (PTSD) is characterized by re-experiencing a traumatic event, avoidance, negative alterations in cognitions and mood, hyperarousal, and severe functional impairment. Women have a two times higher risk of developing PTSD than men. The neurobiological basis for the sex-specific predisposition to PTSD might be related to differences in the functions of stress-responsive systems due to the interaction between gonadal hormones and stress peptides such as corticotropin-releasing factor (CRF), orexin, oxytocin, and neuropeptide Y. Additionally, in phases where estrogens levels are low, the risk of developing or exacerbating PTSD is higher. Most studies have revealed several essential sex differences in CRF function. They include genetic factors, e.g., the CRF promoter contains estrogen response elements. Importantly, sex-related differences are responsible for different predispositions to PTSD and diverse treatment responses. Fear extinction (the process responsible for the effectiveness of behavioral therapy for PTSD) in women during periods of high endogenous estradiol levels (the primary form of estrogens) is reportedly more effective than in periods of low endogenous estradiol. In this review, we present the roles of selected neuropeptides in the sex-related predisposition to PTSD development.
Lymphatic Filariasis: A Systematic Review on Morbidity and Its Repercussions in Countries in the Americas
Authors: Medeiros Zulma M. , Vieira Amanda V. B. , Xavier Amanda T. , Bezerra Gilberto S. N. , Lopes Maria de Fátima C. , Bonfim Cristine V. , Aguiar-Santos Ana M.
Published: December 2021

The Global Program to Eliminate Lymphatic Filariasis (GPELF) is a program that aims to eliminate lymphatic filariasis by 2030. The GPELF strategy is based on interrupting transmission using mass drug administration (MDA) and, in parallel, managing morbidity cases. However, it has been seen that there is a shortage of research in the literature and public policies regarding this last pillar. In this study, we reviewed the literature and available information regarding the burden of filarial morbidity. In addition, we identified that in the Americas, the implementation of structured services with regard to morbidity assistance in the Americas was scarce. We formed a review that aimed to assess the pathogenesis, epidemiology, repercussions, and treatment of filarial morbidity in countries in the Americas where lymphatic filariasis is endemic. Structured searches were carried out on PubMed, LILACS, Scopus, and Web of Science databases without time and language restrictions. Three reviewers evaluated the 2150 studies and performed data extraction, and quality assessment by assigning scores to the studies found. The current literature and available information on the burden of filarial morbidity, as well as the implementation of structured services with regard to morbidity assistance in the Americas, were all found to be scarce. Now that this knowledge gap has been identified, both health services and researchers need to seek the implementation and enhancement of the maintenance of GPELF strategies that relate to the morbidity pillar.
The Value of Meteorological Data in Optimizing the Pattern of Physical Load—A Forecast Model of Rowing Pacing Strategy
Authors: Yan Tian , Zhu Xiaodong , Ding Xuesong , Chen Liming
Published: December 2021

Mastering the information of arena environment is the premise for athletes to optimize their patterns of physical load. Therefore, improving the forecast accuracy of the arena conditions is an urgent task in competitive sports. This paper excavates the meteorological features that have great influence on outdoor events such as rowing and their influence on the pacing strategy. We selected the meteorological data of Tokyo from 1979 to 2020 to forecast the meteorology during the Tokyo 2021 Olympic Games, analyzed the athletes’ pacing choice under different temperatures, humidity and sports levels, and then recommend the best pacing strategy for rowing teams of China. The model proposed in this paper complements the absence of meteorological features in the arena environment assessment and provides an algorithm basis for improving the forecast performance of pacing strategies in outdoor sports.
Towards Child-Appropriate Virtual Acoustic Environments: A Database of High-Resolution HRTF Measurements and 3D-Scans of Children
Authors: Braren Hark Simon , Fels Janina
Published: December 2021

Head-related transfer functions (HRTFs) play a significant role in modern acoustic experiment designs in the auralization of 3-dimensional virtual acoustic environments. This technique enables us to create close to real-life situations including room-acoustic effects, background noise and multiple sources in a controlled laboratory environment. While adult HRTF databases are widely available to the research community, datasets of children are not. To fill this gap, children aged 5–10 years old were recruited among 1st and 2nd year primary school children in Aachen, Germany. Their HRTFs were measured in the hemi-anechoic chamber with a 5-degree × 5-degree resolution. Special care was taken to reduce artifacts from motion during the measurements by means of fast measurement routines. To complement the HRTF measurements with the anthropometric data needed for individualization methods, a high-resolution 3D-scan of the head and upper torso of each participant was recorded. The HRTF measurement took around 3 min. The children’s head movement during that time was larger compared to adult participants in comparable experiments but was generally kept within 5 degrees of rotary and 1 cm of translatory motion. Adult participants only exhibit this range of motion in longer duration measurements. A comparison of the HRTF measurements to the KEMAR artificial head shows that it is not representative of an average child HRTF. Difference can be seen in both the spectrum and in the interaural time delay (ITD) with differences of 70 μs on average and a maximum difference of 138 μs. For both spectrum and ITD, the KEMAR more closely resembles the 95th percentile of range of children’s data. This warrants a closer look at using child specific HRTFs in the binaural presentation of virtual acoustic environments in the future.
UltraViolet SANitizing System for Sterilization of Ambulances Fleets and for Real-Time Monitoring of Their Sterilization Level
Authors: Michelini Zuleika , Mazzei Chiara , Magurano Fabio , Baggieri Melissa , Marchi Antonella , Andreotti Mauro , Cara Andrea , Gaudino Alessandro , Mazzalupi Marco , Antonelli Francesca , Sommella Lorenzo , Angeletti Silvia , Razzano Elena , Runge Arnaud , Petrinca Paolo
Published: December 2021

Background: The contamination of ambulances with pathogenic agents represents a potential threat for the public health, not only for common pathogens but also for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The aim of this project was to exploits the germicidal effect of the UVC radiation at 254 nm to sanitize the patient’s compartment of ambulances with an advanced UltraViolet SANitizing System (UV-SAN) and assess its relevance for avoiding the spread of COVID-19 and other drug resistant pathogens. Methods: The system is equipped with UVC lamps that are activated when the ambulance compartment is empty and sanitize the environment in less than 15 min. An Ozone sensor continuously monitors the gas concentration, ensuring it does not exceed threshold value harmful for patients and operators’ health. The system is relying on GNSS data and a satellite communication link, which allow to monitor and record traceability (when, where and what) of all the sanitation operations performed. This information is real-time monitored from a dedicated web-application. Results: UVC irradiation efficiently reduced SARS-CoV-2 virus titer (>99.99%), on inanimate surfaces such as plastic, stainless steel or rubber, with doses ranging from 5.5 to 24.8 mJ/cm2 and the UV-SAN system is effective against multi drug resistant (MDR) bacteria up to >99.99%, after 10 to 30 min of irradiation. Conclusions: UV-SAN can provide rapid, efficient and sustainable sanitization procedures of ambulances.
Injury Prevalence of the Lower Limbs in Handball Players: A Systematic Review
Authors: Martín-Guzón Iván , Muñoz Alejandro , Lorenzo-Calvo Jorge , Muriarte Diego , Marquina Moisés , de la Rubia Alfonso
Published: December 2021

Lower limb injuries are frequent in handball and a serious hindrance to athletic performance. The aim of this systematic review was to synthesize the available research on the prevalence of lower limb injuries in handball players according to sex and competitive level. According to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis, 19 studies were selected after a systematic search and selection process of three digital databases: Scopus, PubMed, and Web of Science. Furthermore, a study quality analysis using an ‘Extension for Sports Injury and Illness Surveillance of the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE-SIIS)’ was carried out. The sample consisted of 7110 male and female handball players registering 4483 injuries in their lower limbs. The results showed a high incidence of knee injuries (30.23%) and ankle injuries (24.80%), especially in the ligaments, such as the talofibular and the anterior cruciate ligaments. Considering sex and competitive level, knee injuries accounted for 47.02% of injuries among women, while among men, ankle injuries were most prevalent (34.22%) in international competitions. Additionally, the most common cause of injuries was trauma (85.61%). The findings highlighted that the prevalence of lower limb injuries varies greatly according to the characteristics of the sample and injury. Therefore, the study underlines the importance that sports practitioners (physical trainers, readapters, and physiotherapists) adapt training protocols to reduce injury incidence in the most affected body areas or tissues.
The Impact of Environmental Uncertainty on Corporate Innovation: Empirical Evidence from an Emerging Economy
Authors: Chen Jinyong , Shu Weijia , Wang Xiaochi , Sial Muhammad Safdar , Sehleanu Mariana , Badulescu Daniel
Published: December 2021

The paper analyzes the effect of environmental uncertainty on corporate technological innovation from the perspective of an innovation value chain under the institutional background of China. This paper not only discusses the intermediary effect of agency problems on environmental uncertainty and corporate technological innovation but also deeply explores the influence of information transparency, government subsidies, and other mechanisms to alleviate agency problems on environmental uncertainty and corporate technological innovation. We use the data of listed companies in China from 2008 to 2019 as the research sample, and the results show that, in general, environmental uncertainty has a negative effect on both input and output of technological innovation, and the negative effect can last for two years. Further research shows that the agency problem has an intermediary effect on the environmental uncertainty and corporate technology innovation, and the environmental uncertainty aggravates the agency problem, which hinders the input and output of corporate technology innovation. As an important mechanism to alleviate the agency problems, information transparency and government subsidies can effectively alleviate the agency conflict, thus reducing the inhibition of environmental uncertainty on the input and output of technological innovation. Our findings contribute to the discussion of driving factors for technological innovation in the context of China’s system. Our results provide useful insights into the link between environmental uncertainty and corporate innovation for economic academics and practitioners alike.
Cultural Consumption and Knowledge, Attitudes, and Practices Regarding Waste Separation Management in China
Authors: Wang Aiqin , Dang Sijia , Luo Wenying , Ji Kangyuan
Published: December 2021

In 2017, the Chinese government created a policy on mandatory waste separation. Many communities and cities have created waste management institutions and appointed workers to supervise these actions. But there is little information about the situation in terms of the knowledge, attitudes, and practices of waste separation and any differences among regions and cities. Thus, the goal of this paper is to show the current status quo and any differences and to analyze their determinants, especially regarding cultural consumption. Based on online survey data collected in 2021, we found that knowledge in rural regions was lower than in urban regions, but there was no difference in attitudes or practices; the practices in pilot cities were better than in non-pilot cities, but the knowledge and attitudes showed no differences. Different cultural consumption patterns had different impacts on waste separation knowledge, attitudes, and practices. Based on the results, a policy related to culture should be enacted to improve efficiency and increase the action impacts to solve environmental and social issues.
Effects of Body Mass Index, Waist Circumference, Waist-to-Height Ratio and Their Changes on Risks of Dyslipidemia among Chinese Adults: The Guizhou Population Health Cohort Study
Authors: Cao Li , Zhou Jie , Chen Yun , Wu Yanli , Wang Yiying , Liu Tao , Fu Chaowei
Published: December 2021

This study aimed to assess the effects of different anthropometric indices and their changes on the risk of incident dyslipidemia among the Chinese population. From the Guizhou population health cohort study, 2989 Chinese adults without dyslipidemia at baseline were followed up. Anthropometric parameters including waist circumference (WC), body mass index (BMI), waist-to-height ratio (WHtR), and their changes in the latter two indices, and serum lipids were tested after at least 8 h fasting. Hazard ratio (HR), adjusted hazard ratio (aHR), and 95% confidential interval (CI) were calculated to estimate the association between anthropometric parameters and dyslipidemia risk using multivariate Cox regression. A total of 2089 (69.98%) new dyslipidemia cases were identified over an average follow-up of 7.0 years. Baseline BMI (aHR = 1.12, 95%CI 1.01, 1.23) and WHtR (aHR = 1.06, 95%CI 1.00, 1.13) were positively associated with higher risks of incident dyslipidemia but not WC. Each 5.0 kg/m2 increment of BMI or 0.05-unit increment of WHtR was significantly associated with 43% or 25% increased risk of incident dyslipidemia, respectively. The aHRs (95%CI) of incident dyslipidemia for subjects maintaining or developing general obesity were 2.19 (1.53, 3.12) or 1.46 (1.22, 1.75), and 1.54 (1.23, 1.82) or 1.30 (1.06, 1.60) for subjects maintaining or developing abdominal obesity, respectively. Linear trends for aHRs of BMI, WHtR change, and BMI change were observed (p for trend: 0.021, <0.001, <0.001, respectively). BMI, WHtR, and their changes were closely associated with the incidence of dyslipidemia for Chinese adults. Loss in BMI and WHtR had protective effects on incident dyslipidemia, whereas gain of BMI or WHtR increased the dyslipidemia risk. Interventions to control or reduce BMI and WHtR to the normal range are important for the early prevention of dyslipidemia, especially for participants aged 40 years or above, male participants, and urban residents with poor control of obesity.
The Effect of Risk Communication on Public Behavior to Non-Conventional Terrorism—Randomized Control Trial
Authors: Bodas Moran , Ragoler Morel , Rabby Yossi , Krasner Esther
Published: December 2021

Non-conventional terrorism (NCT) incorporates an extended dimension of uncertainty that can lead to fear among the public. Health officials have an unsubstantiated assumption that thousands will seek treatment in hospitals following NCT. This study aims to examine public behavioral intentions in the case of NCT and the effect of risk communication on intents. An online randomized controlled trial was conducted among 1802 adult participants in Israel. Threat perception and behavioral intent before and after exposure to hypothetical NCT scenarios were assessed stratified to the type of media, exposure to rumors, and risk communication. The majority (~64%) of participants are aware of the NCT threat. Almost half (45%) of participants indicated a “high” or “very high” chance of seeking medical attention following an NCT incident. Regression analysis suggests that the odds of participants exposed to risk communication to report an elevated intent of seeking medical attention were 0.470 (95% CI: 0.359, 0.615) times that of participants not exposed to risk communication, χ2 = 30.366, p < 0.001. The findings demonstrate the importance of effective risk communication in reducing undesired public behavior during NCT crises. Efforts must be invested to create a robust risk communication infrastructure to allow the proper management of possible NCT incidents.
Spatiotemporal Polyrhythm Characteristics of Public Bicycle Mobility in Urban Chronotopes Context
Authors: Chen Lijun , Jiang Shangjing
Published: December 2021

Cycling rhythm performance is the result of a complex interplay between active travel demand and cycling network supply. Most studies focused on bicycle flow, but little attention has been paid to cycling rhythm changes for public bicycles. Full sample data of origin–destination enables an efficient description of network-wide cycling mobility efficiency in urban public bicycle systems. In this paper, we show how the spatiotemporal characteristics of cycling speed reveal the performance of cycling rhythms. The inference method of riding speed estimation is proposed with an unknown cycling path. The significant inconsistency of docking stations in cycling rhythm was unraveled by the source–sink relationship comparison. The asymmetry of the cycling rhythm on the path is manifested as the rhythm difference among paths and bidirectional inconsistency. We found that cycling rhythm has a temporal multilayer and spatial mismatch, which shows the inflection points of the cycling rhythm where the travel behavioral preference changes and the exact road segments with different rhythms. This finding suggests that a well-designed cycling environment and occupation-residential function should be considered in active transport demand management and urban planning to help induce active travel behavior decisions.
Cascaded Residual Attention Enhanced Road Extraction from Remote Sensing Images
Authors: Li Shengfu , Liao Cheng , Ding Yulin , Hu Han , Jia Yang , Chen Min , Xu Bo , Ge Xuming , Liu Tianyang , Wu Di
Published: December 2021

Efficient and accurate road extraction from remote sensing imagery is important for applications related to navigation and Geographic Information System updating. Existing data-driven methods based on semantic segmentation recognize roads from images pixel by pixel, which generally uses only local spatial information and causes issues of discontinuous extraction and jagged boundary recognition. To address these problems, we propose a cascaded attention-enhanced architecture to extract boundary-refined roads from remote sensing images. Our proposed architecture uses spatial attention residual blocks on multi-scale features to capture long-distance relations and introduce channel attention layers to optimize the multi-scale features fusion. Furthermore, a lightweight encoder-decoder network is connected to adaptively optimize the boundaries of the extracted roads. Our experiments showed that the proposed method outperformed existing methods and achieved state-of-the-art results on the Massachusetts dataset. In addition, our method achieved competitive results on more recent benchmark datasets, e.g., the DeepGlobe and the Huawei Cloud road extraction challenge.
Automatic Identification of Addresses: A Systematic Literature Review
Authors: Cruz Paula , Vanneschi Leonardo , Painho Marco , Rita Paulo
Published: December 2021

Address matching continues to play a central role at various levels, through geocoding and data integration from different sources, with a view to promote activities such as urban planning, location-based services, and the construction of databases like those used in census operations. However, the task of address matching continues to face several challenges, such as non-standard or incomplete address records or addresses written in more complex languages. In order to better understand how current limitations can be overcome, this paper conducted a systematic literature review focused on automated approaches to address matching and their evolution across time. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were followed, resulting in a final set of 41 papers published between 2002 and 2021, the great majority of which are after 2017, with Chinese authors leading the way. The main findings revealed a consistent move from more traditional approaches to deep learning methods based on semantics, encoder-decoder architectures, and attention mechanisms, as well as the very recent adoption of hybrid approaches making an increased use of spatial constraints and entities. The adoption of evolutionary-based approaches and privacy preserving methods stand as some of the research gaps to address in future studies.
School Location Analysis by Integrating the Accessibility, Natural and Biological Hazards to Support Equal Access to Education
Authors: Sakti Anjar Dimara , Rahadianto Muhammad Ario Eko , Pradhan Biswajeet , Muhammad Hubbi Nashrullah , Andani I Gusti Ayu , Sarli Prasanti Widyasih , Abdillah Muhammad Rais , Anggraini Tania Septi , Purnomo Andhika Dimas , Ridwana Riki , Yulianto Fajar , Manessa Masita Dwi Mandini , Fauziyyah Afina Nur , Yayusman Lissa Fajri , Wikantika Ketut
Published: December 2021

This study proposes a new model for land suitability for educational facilities based on spatial product development to determine the optimal locations for achieving education targets in West Java, Indonesia. Single-aspect approaches, such as accessibility and spatial hazard analyses, have not been widely applied in suitability assessments on the location of educational facilities. Model development was performed based on analyses of the economic value of the land and on the integration of various parameters across three main aspects: accessibility, comfort, and a multi-natural/biohazard (disaster) risk index. Based on the maps of disaster hazards, higher flood-prone areas are found to be in gentle slopes and located in large cities. Higher risks of landslides are spread throughout the study area, while higher levels of earthquake risk are predominantly in the south, close to the active faults and megathrusts present. Presently, many schools are located in very high vulnerability zones (2057 elementary, 572 junior high, 157 senior high, and 313 vocational high schools). The comfort-level map revealed 13,459 schools located in areas with very low and low comfort levels, whereas only 2377 schools are in locations of high or very high comfort levels. Based on the school accessibility map, higher levels are located in the larger cities of West Java, whereas schools with lower accessibility are documented far from these urban areas. In particular, senior high school accessibility is predominant in areas of lower accessibility levels, as there are comparatively fewer facilities available in West Java. Overall, higher levels of suitability are spread throughout West Java. These distribution results revealed an expansion of the availability of schools by area: senior high schools, 303,973.1 ha; vocational high schools, 94,170.51 ha; and junior high schools, 12,981.78 ha. Changes in elementary schools (3936.69 ha) were insignificant, as the current number of elementary schools is relatively much higher. This study represents the first to attempt to integrate these four parameters—accessibility, multi natural hazard, biohazard, comfort index, and land value—to determine potential areas for new schools to achieve educational equity targets.
The Ceramide Synthase Subunit Lac1 Regulates Cell Growth and Size in Fission Yeast
Authors: Flor-Parra Ignacio , Sabido-Bozo Susana , Ikeda Atsuko , Hanaoka Kazuki , Aguilera-Romero Auxiliadora , Funato Kouichi , Muñiz Manuel , Lucena Rafael
Published: December 2021

Cell division produces two viable cells of a defined size. Thus, all cells require mechanisms to measure growth and trigger cell division when sufficient growth has occurred. Previous data suggest a model in which growth rate and cell size are mechanistically linked by ceramide-dependent signals in budding yeast. However, the conservation of mechanisms that govern growth control is poorly understood. In fission yeast, ceramide synthase is encoded by two genes, Lac1 and Lag1. Here, we characterize them by using a combination of genetics, microscopy, and lipid analysis. We showed that Lac1 and Lag1 co-immunoprecipitate and co-localize at the endoplasmic reticulum. However, each protein generates different species of ceramides and complex sphingolipids. We further discovered that Lac1, but not Lag1, is specifically required for proper control of cell growth and size in Schizosaccharomyces pombe. We propose that specific ceramide and sphingolipid species produced by Lac1 are required for normal control of cell growth and size in fission yeast.
Increased Risk of Aortic Dissection with Perlecan Deficiency
Authors: Nonaka Risa , Iesaki Takafumi , Kerever Aurelien , Arikawa-Hirasawa Eri
Published: December 2021

Perlecan (HSPG2), a basement membrane-type heparan sulfate proteoglycan, has been implicated in the development of aortic tissue. However, its role in the development and maintenance of the aortic wall remains unknown. Perlecan-deficient mice (Hspg2−/−-Tg: Perl KO) have been found to show a high frequency (15–35%) of aortic dissection (AD). Herein, an analysis of the aortic wall of Perl KO mice revealed that perlecan deficiency caused thinner and partially torn elastic lamina. Compared to the control aortic tissue, perlecan-deficient aortic tissue showed a significant decrease in desmosine content and an increase in soluble tropoelastin levels, implying the presence of immature elastic fibers in Perl KO mice. Furthermore, the reduced expression of the smooth muscle cell contractile proteins actin and myosin in perlecan-deficient aortic tissue may explain the risk of AD. This study showed that a deficiency in perlecan, which is localized along the elastic lamina and at the interface between elastin and fibrillin-1, increased the risk of AD, largely due to the immaturity of extracellular matrix in the aortic tissue. Overall, we proposed a new model of AD that considers the deficiency of extracellular molecule perlecan as a risk factor.