bluuebunny/crossref_metadata_2025 huggingface dataset:
Recognition of Emotional States Using Multiscale Information Analysis of High Frequency EEG Oscillations
Authors: Gao Zhilin , Cui Xingran , Wan Wang , Gu Zhongze
Published: June 2019

Exploring the manifestation of emotion in electroencephalogram (EEG) signals is helpful for improving the accuracy of emotion recognition. This paper introduced the novel features based on the multiscale information analysis (MIA) of EEG signals for distinguishing emotional states in four dimensions based on Russell’s circumplex model. The algorithms were applied to extract features on the DEAP database, which included multiscale EEG complexity index in the time domain, and ensemble empirical mode decomposition enhanced energy and fuzzy entropy in the frequency domain. The support vector machine and cross validation method were applied to assess classification accuracy. The classification performance of MIA methods (accuracy = 62.01%, precision = 62.03%, recall/sensitivity = 60.51%, and specificity = 82.80%) was much higher than classical methods (accuracy = 43.98%, precision = 43.81%, recall/sensitivity = 41.86%, and specificity = 70.50%), which extracted features contain similar energy based on a discrete wavelet transform, fractal dimension, and sample entropy. In this study, we found that emotion recognition is more associated with high frequency oscillations (51–100Hz) of EEG signals rather than low frequency oscillations (0.3–49Hz), and the significance of the frontal and temporal regions are higher than other regions. Such information has predictive power and may provide more insights into analyzing the multiscale information of high frequency oscillations in EEG signals.
Urban Crowd Detection Using SOM, DBSCAN and LBSN Data Entropy: A Twitter Experiment in New York and Madrid
Authors: Sakkari Mohamed , D. Algarni Abeer , Zaied Mourad
Published: June 2019

The surfer and the physical location are two important concepts associated with each other in the social network-based localization service. This work consists of studying urban behavior based on location-based social networks (LBSN) data; we focus especially on the detection of abnormal events. The proposed crowd detection system uses the geolocated social network provided by the Twitter application programming interface (API) to automatically detect the abnormal events. The methodology we propose consists of using an unsupervised competitive learning algorithm (self-organizing map (SOM)) and a density-based clustering method (density-based spatial clustering of applications with noise (DBCSAN)) to identify and detect crowds. The second stage is to build the entropy model to determine whether the detected crowds fit into the daily pattern with reference to a spatio-temporal entropy model, or whether they should be considered as evidence that something unusual occurs in the city because of their number, size, location and time of day. To detect an abnormal event in the city, it is sufficient to determine the real entropy model and to compare it with the reference model. For the normal day, the reference model is constructed offline for each time interval. The obtained results confirm the effectiveness of our method used in the first stage (SOM and DBSCAN stage) to detect and identify clusters dynamically, and imitating human activity. These findings also clearly confirm the detection of special days in New York City (NYC), which proves the performance of our proposed model.
Construction of Residue Number System Using Hardware Efficient Diagonal Function
Authors: Valueva Maria , Valuev Georgii , Semyonova Nataliya , Lyakhov Pavel , Chervyakov Nikolay , Kaplun Dmitry , Bogaevskiy Danil
Published: June 2019

The residue number system (RNS) is a non-positional number system that allows one to perform addition and multiplication operations fast and in parallel. However, because the RNS is a non-positional number system, magnitude comparison of numbers in RNS form is impossible, so a division operation and an operation of reverse conversion into a positional form containing magnitude comparison operations are impossible too. Therefore, RNS has disadvantages in that some operations in RNS, such as reverse conversion into positional form, magnitude comparison, and division of numbers are problematic. One of the approaches to solve this problem is using the diagonal function (DF). In this paper, we propose a method of RNS construction with a convenient form of DF, which leads to the calculations modulo 2 n , 2 n − 1 or 2 n + 1 and allows us to design efficient hardware implementations. We constructed a hardware simulation of magnitude comparison and reverse conversion into a positional form using RNS with different moduli sets constructed by our proposed method, and used different approaches to perform magnitude comparison and reverse conversion: DF, Chinese remainder theorem (CRT) and CRT with fractional values (CRTf). Hardware modeling was performed on Xilinx Artix 7 xc7a200tfbg484-2 in Vivado 2016.3 and the strategy of synthesis was highly area optimized. The hardware simulation of magnitude comparison shows that, for three moduli, the proposed method allows us to reduce hardware resources by 5.98–49.72% in comparison with known methods. For the four moduli, the proposed method reduces delay by 4.92–21.95% and hardware costs by twice as much by comparison to known methods. A comparison of simulation results from the proposed moduli sets and balanced moduli sets shows that the use of these proposed moduli sets allows up to twice the reduction in circuit delay, although, in several cases, it requires more hardware resources than balanced moduli sets.
Wearable Travel Aid for Environment Perception and Navigation of Visually Impaired People
Authors: Bai Jinqiang , Liu Zhaoxiang , Lin Yimin , Li Ye , Lian Shiguo , Liu Dijun
Published: June 2019

Assistive devices for visually impaired people (VIP) which support daily traveling and improve social inclusion are developing fast. Most of them try to solve the problem of navigation or obstacle avoidance, and other works focus on helping VIP to recognize their surrounding objects. However, very few of them couple both capabilities (i.e., navigation and recognition). Aiming at the above needs, this paper presents a wearable assistive device that allows VIP to (i) navigate safely and quickly in unfamiliar environment, and (ii) to recognize the objects in both indoor and outdoor environments. The device consists of a consumer Red, Green, Blue and Depth (RGB-D) camera and an Inertial Measurement Unit (IMU), which are mounted on a pair of eyeglasses, and a smartphone. The device leverages the ground height continuity among adjacent image frames to segment the ground accurately and rapidly, and then search the moving direction according to the ground. A lightweight Convolutional Neural Network (CNN)-based object recognition system is developed and deployed on the smartphone to increase the perception ability of VIP and promote the navigation system. It can provide the semantic information of surroundings, such as the categories, locations, and orientations of objects. Human–machine interaction is performed through audio module (a beeping sound for obstacle alert, speech recognition for understanding the user commands, and speech synthesis for expressing semantic information of surroundings). We evaluated the performance of the proposed system through many experiments conducted in both indoor and outdoor scenarios, demonstrating the efficiency and safety of the proposed assistive system.
Investigation of Combustion Properties and Soot Deposits of Various US Crude Oils
Authors: Singh Gurjap , Esmaeilpour Mehdi , Ratner Albert
Published: June 2019

The oil boom in the North Dakota oilfields has resulted in improved energy security for the US. Recent estimates of oil production rates indicate that even completion of the Keystone XL pipeline will only fractionally reduce the need to ship this oil by rail. Current levels of oil shipment have already caused significant strain on rail infrastructure and led to crude oil train derailments, resulting in loss of life and property. Treating crude oil as a multicomponent liquid fuel, this work aims to understand crude oil droplet burning and thereby lead to methods to improve train fire safety. Sub-millimeter sized droplets of Pennsylvania, Texas, Colorado, and Bakken crude were burned, and the process was recorded with charge-couple device (CCD) and complementary metal-oxide semiconductor (CMOS) high-speed cameras. The resulting images were post-processed to obtain various combustion parameters, such as burning rate, ignition delay, total combustion time, and microexplosion behavior. The soot left behind was analyzed using a Scanning Electron Microscope (SEM). This data is expected be used for validation of combustion models for complex multicomponent liquid fuels, and subsequently in the modification of combustion properties of crude oil using various additives to make it safer to transport.
A Three-Level Universal Electric Vehicle Charger Based on Voltage-Oriented Control and Pulse-Width Modulation
Authors: Al-Ogaili Ali Saadon , Aris Ishak Bin , Verayiah Renuga , Ramasamy Agileswari , Marsadek Marayati , Rahmat Nur Azzammudin , Hoon Yap , Aljanad Ahmed , Al-Masri Ahmed N.
Published: June 2019

Electric vehicles (EVs) could be used to address the issues of environmental pollution and the depletion of non-renewable energy resources. EVs, which are energized by a battery storage system, are becoming attractive because they keep the environment clean. Furthermore, the cost of EVs is becoming cheaper. Thus, EVs will become a significant load on utility distribution system in the future. EV chargers play a significant role in the expansion of EVs. The input current of an EV charger with a high total harmonic distortion (THD) and a high ripple distortion of the output voltage can impact battery life and battery charging time. Furthermore, the high cost and large size of the chargers are considered other issues in EV development. This work presents the complete design process of a universal EV charger with a special focus on its control algorithms. In this regard, a novel control algorithm based on the integration of voltage-oriented control (VOC) and the sinusoidal pulse-width modulation (SPWM) technique is proposed to ensure effective Levels 1, 2, and 3 battery charging. A simulation of the universal EV charger was conducted and assessed in MATLAB–Simulink. Moreover, a laboratory prototype was constructed with a TMS320F28335 digital signal processor (DSP) programmed as the controller to validate its operation and performance. The findings show that the proposed charger is able to provide a controllable and constant charging voltage for a variety of EVs, with an input current of low total harmonic distortion (THD) and an almost unity power factor.
Energy and Exergy Analysis of Different Exhaust Waste Heat Recovery Systems for Natural Gas Engine Based on ORC
Authors: Valencia Guillermo , Fontalvo Armando , Cárdenas Yulineth , Duarte Jorge , Isaza Cesar
Published: June 2019

Waste heat recovery (WHR) from exhaust gases in natural gas engines improves the overall conversion efficiency. The organic Rankine cycle (ORC) has emerged as a promising technology to convert medium and low-grade waste heat into mechanical power and electricity. This paper presents the energy and exergy analyses of three ORC–WHR configurations that use a coupling thermal oil circuit. A simple ORC (SORC), an ORC with a recuperator (RORC), and an ORC with double-pressure (DORC) configuration are considered; cyclohexane, toluene, and acetone are simulated as ORC working fluids. Energy and exergy thermodynamic balances are employed to evaluate each configuration performance, while the available exhaust thermal energy variation under different engine loads is determined through an experimentally validated mathematical model. In addition, the effect of evaporating pressure on the net power output, thermal efficiency increase, specific fuel consumption, overall energy conversion efficiency, and exergy destruction is also investigated. The comparative analysis of natural gas engine performance indicators integrated with ORC configurations present evidence that RORC with toluene improves the operational performance by achieving a net power output of 146.25 kW, an overall conversion efficiency of 11.58%, an ORC thermal efficiency of 28.4%, and a specific fuel consumption reduction of 7.67% at a 1482 rpm engine speed, a 120.2 L/min natural gas flow, 1.784 lambda, and 1758.77 kW of mechanical engine power.
Eucalyptus Short-Rotation Management Effects on Nutrient and Sediments in Subtropical Streams
Authors: Rodrigues Carolina Bozetti , Taniwaki Ricardo Hideo , Lane Patrick , Lima Walter de Paula , Ferraz Silvio Frosini de Barros
Published: June 2019

Forested catchments generally present conserved aquatic ecosystems without anthropogenic disturbances; however, forest management operations can degrade these environments, including their water quality. Despite the potential degradation, few studies have analyzed the effects of forest management in subtropical regions, especially in forest plantations with intensive management, such as Eucalyptus plantations in Brazil. The intensive management of those plantations is characterized by fast-growing, short rotation cycles, and high productivity. This study aimed to assess the effects of Eucalyptus plantations harvesting on the concentration and exportation of nutrients and suspended solids in subtropical streams. Results showed that clear-cut harvesting and subsequent forest management operations do not alter most of the concentration of nitrate, potassium, calcium, and magnesium. The concentration of suspended solids increased during the first year after timber harvesting in all studied catchments, however, the increases were statistically significant in only two catchments. In the first year after harvest, it was observed an increment of water yield/precipitation ratio at three catchments, which also increased export of nutrients and suspended solids. Our results showed that harvesting of fast-growing Eucalyptus forest plantations partially affected sediment exports and did not compromise water quality in the studied catchments. However, the catchment land-use design, especially related to road density and land-use composition, showed significant relationship with sediment exportation.
LANDFIRE Remap Prototype Mapping Effort: Developing a New Framework for Mapping Vegetation Classification, Change, and Structure
Authors: Picotte Joshua J. , Dockter Daryn , Long Jordan , Tolk Brian , Davidson Anne , Peterson Birgit
Published: June 2019

LANDFIRE (LF) National (2001) was the original product suite of the LANDFIRE program, which included Existing Vegetation Cover (EVC), Height (EVH), and Type (EVT). Subsequent refinements after feedback from data users resulted in updated products, referred to as LF 2001, that now served as LANDFIRE’s baseline datasets and are the basis for all subsequent LANDFIRE updates. These updates account for disturbances and vegetation transition changes that may not represent current vegetation conditions. Therefore, in 2016 LANDFIRE initiated the Remap prototype to determine how to undertake a national-scale remap of the LANDFIRE primary vegetation datasets. EVC, EVH, and EVT were produced (circa 2015) via modeling for ecologically variable prototyping areas in the Pacific Northwest (NW) and Grand Canyon (GC). An error analysis within the GC suggested an overall accuracy of 52% (N = 800) for EVT, and a goodness of fit of 51% (N = 38) for percent cover (continuous EVC) and 53% (N = 38) for height (continuous EVH). The prototyping effort included a new 81-class map using the National Vegetation Classification (NVC) within the NW. This paper presents a narrative of the innovative methodologies in image processing and mapping used to create the new LANDFIRE vegetation products.
A Rapid and Accurate MinION-Based Workflow for Tracking Species Biodiversity in the Field
Authors: Maestri Simone , Cosentino Emanuela , Paterno Marta , Freitag Hendrik , Garces Jhoana M. , Marcolungo Luca , Alfano Massimiliano , Njunjić Iva , Schilthuizen Menno , Slik Ferry , Menegon Michele , Rossato Marzia , Delledonne Massimo
Published: June 2019

Genetic markers (DNA barcodes) are often used to support and confirm species identification. Barcode sequences can be generated in the field using portable systems based on the Oxford Nanopore Technologies (ONT) MinION sequencer. However, to achieve a broader application, current proof-of-principle workflows for on-site barcoding analysis must be standardized to ensure a reliable and robust performance under suboptimal field conditions without increasing costs. Here, we demonstrate the implementation of a new on-site workflow for DNA extraction, PCR-based barcoding, and the generation of consensus sequences. The portable laboratory features inexpensive instruments that can be carried as hand luggage and uses standard molecular biology protocols and reagents that tolerate adverse environmental conditions. Barcodes are sequenced using MinION technology and analyzed with ONTrack, an original de novo assembly pipeline that requires as few as 1000 reads per sample. ONTrack-derived consensus barcodes have a high accuracy, ranging from 99.8 to 100%, despite the presence of homopolymer runs. The ONTrack pipeline has a user-friendly interface and returns consensus sequences in minutes. The remarkable accuracy and low computational demand of the ONTrack pipeline, together with the inexpensive equipment and simple protocols, make the proposed workflow particularly suitable for tracking species under field conditions.
Evaluation of Different Pooling Methods to Establish a Multi-Century δ18O Chronology for Paleoclimate Reconstruction
Authors: Foroozan Zeynab , Grießinger Jussi , Pourtahmasi Kambiz , Bräuning Achim
Published: June 2019

To develop multi-century stable isotope chronologies from tree rings, pooling techniques are applied to reduce laboratory costs and time. However, pooling of wood samples from different trees may have adverse effects on the signal amplitude in the final isotope chronology. We tested different pooling approaches to identify the method that is most cost-efficient, without compromising the ability of the final chronology to reflect long-term climate variability as well as climatic extreme years. As test material, we used δ18O data from juniper trees (Juniperus polycarpus) from Northern Iran. We compared inter-tree and shifted 5-year blocks serial pooling of stable isotope series from 5 individual trees and addition of one single series to a shifted serial pooled chronology. The inter-tree pooled chronology showed the strongest climate sensitivity and most synchronous δ18O variations with the individual tree ring analyses, while the shifted block chronologies showed a marked decline in high-frequency signals and no correlations with climate variables of the growth year. Combinations of block-pooled and single isotope series compensated the high-frequency decline but added tree-individual climatic signals. Therefore, we recommend pooling calendar synchronous tree rings from individual trees as a viable alternative to individual-tree isotope measurements for robust paleoclimate reconstructions.
Evidence for Pathways of Concentrated Submarine Groundwater Discharge in East Antarctica from Helicopter-Borne Electrical Resistivity Measurements
Authors: Foley Neil , Tulaczyk Slawek M. , Grombacher Denys , Doran Peter T. , Mikucki Jill , Myers Krista F. , Foged Nikolaj , Dugan Hilary , Auken Esben , Virginia Ross
Published: June 2019

The Southern Ocean receives limited liquid surface water input from the Antarctic continent. It has been speculated, however, that significant liquid water may flow from beneath the Antarctic Ice Sheet, and that this subglacial flow carries that water along with dissolved nutrients to the coast. The delivery of solutes, particularly limiting nutrients like bioavailable iron, to the Southern Ocean may contribute to ecosystem processes including primary productivity. Using a helicopter-borne time domain electromagnetic survey along the coastal margins of the McMurdo Dry Valleys region of Southern Victoria Land, Antarctica, we detected subsurface connections between inland lakes, aquifers, and subglacial waters. These waters, which appear as electrically conductive anomalies, are saline and may contain high concentrations of biologically important ions, including iron and silica. Local hydraulic gradients may drive these waters to the coast, where we postulate they emerge as submarine groundwater discharge. This high latitude groundwater system, imaged regionally in the McMurdo Dry Valleys, may be representative of a broader system of Antarctic submarine groundwater discharge that fertilizes the Southern Ocean. In total, it has the potential to deliver tens of gigagrams of bioavailable Fe and Si to the coastal zone.
The Prevalence of Fetal Alcohol Spectrum Disorders in An American Indian Community
Authors: Montag Annika , Romero Rhonda , Jensen Toni , Goodblanket Amiyonette , Admire Ami , Whitten Conner , Calac Daniel , Akshoomoff Natacha , Sanchez Maria , Zacarias MarLa , Zellner Jennifer , del Campo Miguel , Jones Kenneth , Chambers Christina
Published: June 2019

The prevalence of fetal alcohol spectrum disorders (FASD) differs among populations and is largely unknown among minority populations. Prevalence and characterization of FASD is necessary for prevention efforts and allocation of resources for treatment and support. However, prevalence data are lacking, including among many minority populations. The aim of this study was to obtain an FASD prevalence estimate in a Southern California American Indian community employing active case-ascertainment. In 2016, American Indian children aged 5–7 years and their caregivers were recruited in collaboration with Southern California Tribal Health Clinic. Children were assessed using physical examinations and neurobehavioral testing. Parent or guardian interviews assessed child behavior and prenatal exposures including alcohol. Of 488 children identified as eligible to participate, 119 families consented and 94 completed assessments to allow a classification for FASD. Participating children (n = 94) were an average of 6.61 ± 0.91 years old and half were female. Most interviews were conducted with biological mothers (85.1%). Less than one third (29.8%) of mothers reported consuming any alcohol in pregnancy and 19.1% met study criteria for risky alcohol exposure prior to pregnancy recognition. Overall 20 children met criteria for FASD, resulting in an estimated minimum prevalence of 41.0 per 1000 (4.1%). No cases of fetal alcohol syndrome (FAS) were identified; 14 (70.0%) met criteria for alcohol related neuro- developmental disorder (ARND). Minimum prevalence estimates found in this sample are consistent with those noted in the general population.
Obesity and Polypharmacy among African American Older Adults: Gender as the Moderator and Multimorbidity as the Mediator
Authors: Assari Shervin , Wisseh Cheryl , Bazargan Mohsen
Published: June 2019

Despite high prevalence of obesity and polypharmacy among African American (AA) older adults, little information exists on the associations between the two in this population. This study explored the association between obesity and polypharmacy among AA older adults who were residing in poor urban areas of South Los Angeles. We also investigated role of gender as the moderator and multimorbidity as the mediator of this association. In a community-based study in South Los Angeles, 308 AA older adults (age ≥ 55 years) were entered into this study. From this number, 112 (36.4%) were AA men and 196 (63.6%) were AA women. Polypharmacy (taking 5+ medications) was the dependent variable, obesity was the independent variable, gender was the moderator, and multimorbidity (number of chronic medical conditions) was the mediator. Age, educational attainment, financial difficulty (difficulty paying bills, etc.), income, marital status, self-rated health (SRH), and depression were the covariates. Logistic regressions were used for data analyses. In the absence of multimorbidity in the model, obesity was associated with higher odds of polypharmacy in the pooled sample. This association was not significant when we controlled for multimorbidity, suggesting that multimorbidity mediates the obesity-polypharmacy link. We found significant association between obesity and polypharmacy in AA women not AA men, suggesting that gender moderates such association. AA older women with obesity are at a higher risk of polypharmacy, an association which is mainly due to multimorbidity. There is a need for screening for inappropriate polypharmacy in AA older women with obesity and associated multimorbidity.
Interpersonal Conflict, School Connectedness and Depressive Symptoms in Chinese Adolescents: Moderation Effect of Gender and Grade Level
Authors: He Guan-Hao , Strodl Esben , Chen Wei-Qing , Liu Fan , Hayixibayi Alimila , Hou Xiang-Yu
Published: June 2019

This study examined the associations of interpersonal conflict and school connectedness with depressive symptoms in Chinese adolescents. A cross-sectional study was conducted among 6576 adolescents in Shenzhen, China. Participants completed a battery of questionnaires that assessed adolescents’ depressive symptoms, conflict with parents, teachers, and peers, school connectedness, and demographics. Multiple linear regression analysis was used to explore the association of interpersonal conflict and school connectedness with depressive symptoms in adolescents. Results showed that conflicts with their mother, father, teachers, and peers were associated with higher levels of depressive symptoms in adolescents, while greater school connectedness was related to lower levels of depressive symptoms in adolescents. Gender proved to be a moderator of these relationships in that the associations of quarreling with mothers, mothers’ use of emotional punishments, teachers’ use of emotional punishments, and school connectedness with depressive symptoms were stronger in females than males. Moreover, grade level proved to be another moderator, with the associations of teachers’ use of physical punishment and fighting with peers with depressive symptoms being stronger in primary school students than in secondary school students. Our findings suggest that gender and grade level moderated the association of interpersonal conflict and school connectedness with depressive symptoms in Chinese adolescents.
Impact of Lifting of Two Types of Barrels on Postural Control, Trunk Muscle Recruitment, and Kinematic Measures in Manual Workers
Authors: Cavaguchi Amanda M.S. , Oliveira Márcio R. , Macedo Christiane G. , Souza Pablo E.A. de , Aguiar Andreo F. , Dallaire Mathieu , Ngomo Suzy , Silva Rubens A. da
Published: June 2019

The aim of this study was to evaluate the impact of 2 types of beer barrels on postural control, trunk activation, and kinematic measures in adult workers. Twelve (12) males randomly performed 4 tasks on a force platform for 20 s: (1) hold an empty recyclable barrel, (2) hold a full recyclable barrel (30 L), (3) hold an empty steel barrel, and (4) hold a full steel barrel (30 L). Trunk muscular activation, force platform and kinematic measures at the trunk, hip, and knee joints were computed. The full steel barrel produced greater postural oscillation than other conditions. Higher trunk activity was also reported during the full steel barrel task. Significant kinematic changes only in the trunk were observed between the empty steel barrel and the full recyclable barrel tasks. In conclusion, the full steel barrel produced a negative impact on postural control, increasing trunk activity and changing trunk flexion angle in adult workers.
Preparation of Attapulgite/CoFe2O4 Magnetic Composites for Efficient Adsorption of Tannic Acid from Aqueous Solution
Authors: Teng Yue , Liu Zhiying , Yao Ke , Song Wenbin , Sun Yongjun , Wang Hailing , Xu Yanhua
Published: June 2019

The use of attapulgite (ATP)-based materials for adsorption of pollutants from water and wastewater has received growing attention. However, recovering ATP-based adsorbents remains a challenge. In this study, a magnetic adsorbent ATP/CoFe2O4 with high tannic acid (TA) adsorptive capacity was fabricated via a facile co-precipitation approach and was well characterized. The loaded CoFe2O4 particles were embedded into the adsorbent surfaces to allow magnetic separability. For this material, its TA adsorption kinetics, isotherm behavior, and magnetic separation efficiency are reported. The developed magnetic composites had rapid sorption kinetics of 3 h, high sorption capacity of 109.36 mg/g, and good magnetic separation efficiency of 80%. The used ATP/CoFe2O4 was successfully regenerated by NaOH and reused five times without a substantial reduction in TA removal and magnetic performance. Intermolecular hydrogen bonding formation and surface complexation were identified as the sorption mechanisms of TA by ATP/CoFe2O4.
Incidence and Characteristics of Endophthalmitis after Cataract Surgery in Poland, during 2010–2015
Authors: Nowak Michał S. , Grzybowski Andrzej , Michalska-Małecka Katarzyna , Szaflik Jacek P. , Kozioł Milena , Niemczyk Wojciech , Grabska-Liberek Iwona
Published: June 2019

Background: The assessment of the incidence and characteristic of acute and chronic postoperative endophthalmitis (POE) after cataract surgery in Poland during 2010–2015. Patients and methods: All hospitalizations of patients, in the National Database of Hospitalizations, who underwent cataract surgery alone or in combined procedures in Poland between January 2010 and December 2015, with a billing code of endophthalmitis, were selected. Acute endophthalmitis was identified if symptoms occurred within 1–42 days from the cataract surgery and chronic endophthalmitis if symptoms occurred ≥43 days after cataract surgery, respectively. Results: In total, 1331 cases of POE after 1,218,777 cataract extractions were identified. The overall incidence of POE decreased from 0.125% in 2010 to 0.066% in 2015. In multiple logistic regression analyses, increasing age was significantly associated with acute POE, while type II diabetes mellitus, extracapsular cataract extraction, and one-day surgery were significantly associated with chronic POE. The combined cataract surgery and male sex were significant risk factors for both acute and chronic POE. A total of 62.51% of all eyes affected by POE received antibiotic treatment and 37.49% had vitrectomy treatment. Conclusions: During the study period, the total incidence of postoperative endophthalmitis after cataract surgery decreased significantly.
New Aspects of Vitamin K Research with Synthetic Ligands: Transcriptional Activity via SXR and Neural Differentiation Activity
Authors: Hirota Yoshihisa , Suhara Yoshitomo
Published: June 2019

Vitamin K is classified into three homologs depending on the side-chain structure, with 2-methyl-1,4-naphthoqumone as the basic skeleton. These homologs are vitamin K1 (phylloquinone: PK), derived from plants with a phythyl side chain; vitamin K2 (menaquinone-n: MK-n), derived from intestinal bacteria with an isoprene side chain; and vitamin K3 (menadione: MD), a synthetic product without a side chain. Vitamin K homologs have physiological effects, including in blood coagulation and in osteogenic activity via γ-glutamyl carboxylase and are used clinically. Recent studies have revealed that vitamin K homologs are converted to MK-4 by the UbiA prenyltransferase domain-containing protein 1 (UBIAD1) in vivo and accumulate in all tissues. Although vitamin K is considered to have important physiological effects, its precise activities and mechanisms largely remain unclear. Recent research on vitamin K has suggested various new roles, such as transcriptional activity as an agonist of steroid and xenobiotic nuclear receptor and differentiation-inducing activity in neural stem cells. In this review, we describe synthetic ligands based on vitamin K and exhibit that the strength of biological activity can be controlled by modification of the side chain part.
Novel Selective Estrogen Receptor Modulator Ameliorates Murine Colitis
Authors: Polari Lauri , Anttila Santeri , Helenius Terhi , Wiklund Anu , Linnanen Tero , Toivola Diana M. , Määttä Jorma
Published: June 2019

Estrogen-receptor-mediated signaling has been suggested to decrease the inflammatory response in monocyte macrophages. Previously, we showed that a novel selective estrogen receptor modulator (SERM2) promotes anti-inflammatory phenotype of monocytes in vitro. In this study, we demonstrate the potential of SERM2 in amelioration of colitis. We utilized a dextran sodium sulfate (DSS)-induced colitis model in FVB/n mice to demonstrate the effects of orally administered SERM2 on the clinical status of the mice and the histopathological changes in the colon, as well as proportion of Mrc-1 positive macrophages. SERM2 nuclear receptor affinities were measured by radioligand binding assays. Orally administered, this compound significantly alleviated DSS-induced colitis in male mice and induced local estrogen receptor activation in the inflamed colon, as well as promoting anti-inflammatory cytokine expression and infiltration of anti-inflammatory monocytes. We show that this novel drug candidate has an affinity to estrogen receptors α and β and progesterone receptors, but not to glucocorticoid receptor, thus expressing unique binding properties compared to other sex steroid receptor ligands. These results indicate that novel drug candidates to alleviate inflammatory conditions of the colon could be found among sex steroid receptor activating compounds.
Molybdenum-Induced Effects on Nitrogen Metabolism Enzymes and Elemental Profile of Winter Wheat (Triticum aestivum L.) Under Different Nitrogen Sources
Authors: Imran Muhammad , Sun Xuecheng , Hussain Saddam , Ali Usman , Rana Muhammad Shoaib , Rasul Fahd , Saleem Muhammad Hamzah , Moussa Mohamed G. , Bhantana Parashuram , Afzal Javaria , Elyamine Ali Mohamed , Hu Cheng Xiao
Published: June 2019

Different nitrogen (N) sources have been reported to significantly affect the activities and expressions of N metabolism enzymes and mineral elements concentrations in crop plants. However, molybdenum-induced effects in winter wheat cultivars have still not been investigated under different N sources. Here, a hydroponic study was carried out to investigate these effects on two winter wheat cultivars (‘97003’ and ‘97014’) as Mo-efficient and Mo-inefficient, respectively, under different N sources (NO3−, NH4NO3, and NH4+). The results revealed that the activities of nitrate reductase (NR) and nitrite reductase (NiR) followed the order of NH4NO3 > NO3− > NH4+ sources, while glutamine synthetase (GS) and glutamate synthase (GOGAT) followed the order of NH4+ > NH4NO3 > NO3− in both the wheat cultivars. However, Mo-induced effects in the activities and expressions of N metabolism enzymes under different N sources followed the order of NH4NO3 > NO3− > NH4+ sources, indicating that Mo has more complementary effects towards nitrate nutrition than the sole ammonium source in winter wheat. Interestingly, under −Mo-deprived conditions, cultivar ‘97003’ recorded more pronounced alterations in Mo-dependent parameters than ‘97014’ cultivar. Moreover, Mo application increased the proteins, amino acids, ammonium, and nitrite contents while concomitantly decreasing the nitrate contents in the same order of NH4NO3 > NO3− > NH4+ sources that coincides with the Mo-induced N enzymes activities and expressions. The findings of the present study indicated that Mo plays a key role in regulating the N metabolism enzymes and assimilatory products under all the three N sources; however, the extent of complementation exists in the order of NH4NO3 > NO3− > NH4+ sources in winter wheat. In addition, it was revealed that mineral elements profiles were mainly affected by different N sources, while Mo application generally had no significant effects on the mineral elements contents in the winter wheat leaves under different N sources.
NOS2 inhibitor 1400W Induces Autophagic Flux and Influences Extracellular Vesicle Profile in Human Glioblastoma U87MG Cell Line
Authors: Palumbo Paola , Lombardi Francesca , Augello Francesca Rosaria , Giusti Ilaria , Luzzi Sabino , Dolo Vincenza , Cifone Maria Grazia , Cinque Benedetta
Published: June 2019

The relevance of nitric oxide synthase 2 (NOS2) as a prognostic factor in Glioblastoma Multiforme (GBM) malignancy is emerging. We analyzed the effect of NOS2 inhibitor 1400W on the autophagic flux and extracellular vesicle (EV) secretion in U87MG glioma cells. The effects of glioma stem cells (GSC)-derived EVs on adherent U87MG were evaluated. Cell proliferation and migration were examined while using Cell Counting Kit-8 assay (CCK-8) and scratch wound healing assay. Cell cycle profile and apoptosis were analyzed by flow cytometry. Autophagy-associated acidic vesicular organelles were detected and quantified by acridine orange staining. The number and size of EVs were assessed by nanoparticle tracking analysis. EV ultrastructure was verified by transmission electron microscopy (TEM). WB was used to analyze protein expression and acid sphingomyelinase was determined through ceramide levels. 1400W induced autophagy and EV secretion in both adherent U87MG and GSCs. EVs secreted by 1400W-treated GSC, but not those from untreated cells, were able to inhibit adherent U87MG cell growth and migration while also inducing a relevant level of autophagy. The hypothesis of NOS2 expression as GBM profile marker or interesting therapeutic target is supported by our findings. Autophagy and EV release following treatment with the NOS2 inhibitor could represent useful elements to better understand the complex biomolecular frame of GBM.
Recent Advances in Models, Mechanisms, Biomarkers, and Interventions in Cisplatin-Induced Acute Kidney Injury
Authors: Holditch Sara J. , Brown Carolyn N. , Lombardi Andrew M. , Nguyen Khoa N. , Edelstein Charles L.
Published: June 2019

Cisplatin is a widely used chemotherapeutic agent used to treat solid tumours, such as ovarian, head and neck, and testicular germ cell. A known complication of cisplatin administration is acute kidney injury (AKI). The development of effective tumour interventions with reduced nephrotoxicity relies heavily on understanding the molecular pathophysiology of cisplatin-induced AKI. Rodent models have provided mechanistic insight into the pathophysiology of cisplatin-induced AKI. In the subsequent review, we provide a detailed discussion of recent advances in the cisplatin-induced AKI phenotype, principal mechanistic findings of injury and therapy, and pre-clinical use of AKI rodent models. Cisplatin-induced AKI murine models faithfully develop gross manifestations of clinical AKI such as decreased kidney function, increased expression of tubular injury biomarkers, and tubular injury evident by histology. Pathways involved in AKI include apoptosis, necrosis, inflammation, and increased oxidative stress, ultimately providing a translational platform for testing the therapeutic efficacy of potential interventions. This review provides a discussion of the foundation laid by cisplatin-induced AKI rodent models for our current understanding of AKI molecular pathophysiology.
Enhanced Hepatogenic Differentiation of Human Wharton’s Jelly–Derived Mesenchymal Stem Cells by Using Three-Step Protocol
Authors: Panta Wachira , Imsoonthornruksa Sumeth , Yoisungnern Ton , Suksaweang Sanong , Ketudat-Cairns Mariena , Parnpai Rangsun
Published: June 2019

Currently, human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) are an attractive source of stem cells for cell-based therapy, owing to their ability to undergo self-renewal and differentiate into all mesodermal, some neuroectodermal, and endodermal progenies, including hepatocytes. Herein, this study aimed to investigate the effects of sodium butyrate (NaBu), an epigenetic regulator that directly inhibits histone deacetylase, on hepatic endodermal lineage differentiation of hWJ-MSCs. NaBu, at 1 mM, optimally promoted endodermal differentiation of hWJ-MSCs, along with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) supplementation. CXCR4, HNF3β, SOX17 (endodermal), and GATA6 (mesendodermal) mRNAs were also up-regulated (p < 0.001). Immunocytochemistry and a Western blot analysis of SOX17 and HNF3β confirmed that the 1 mM NaBu along with EGF and bFGF supplementation condition was appropriately pre-treated with hWJ-MSCs before hepatogenic differentiation. Furthermore, the hepatic differentiation medium with NaBu pre-treatment up-regulated hepatoblast (AFP and HNF3β) and hepatic (CK18 and ALB) markers, and increased the proportion of mature hepatocyte functions, including G6P, C/EBPα, and CYP2B6 mRNAs, glycogen storage and urea secretion. The hepatic differentiation medium with NaBu in the pre-treatment step can induce hWJ-MSC differentiation toward endodermal, hepatoblastic, and hepatic lineages. Therefore, the hepatic differentiation medium with NaBu pre-treatment for differentiating hWJ-MSCs could represent an alternative protocol for cell-based therapy and drug screening in clinical applications.
Is It Possible to Predict the Odor of a Molecule on the Basis of its Structure?
Authors: Genva Manon , Kenne Kemene Tierry , Deleu Magali , Lins Laurence , Fauconnier Marie-Laure
Published: June 2019

The olfactory sense is the dominant sensory perception for many animals. When Richard Axel and Linda B. Buck received the Nobel Prize in 2004 for discovering the G protein-coupled receptors’ role in olfactory cells, they highlighted the importance of olfaction to the scientific community. Several theories have tried to explain how cells are able to distinguish such a wide variety of odorant molecules in a complex context in which enantiomers can result in completely different perceptions and structurally different molecules. Moreover, sex, age, cultural origin, and individual differences contribute to odor perception variations that complicate the picture. In this article, recent advances in olfaction theory are presented, and future trends in human olfaction such as structure-based odor prediction and artificial sniffing are discussed at the frontiers of chemistry, physiology, neurobiology, and machine learning.
Phylogenomics Provides New Insights into Gains and Losses of Selenoproteins among Archaeplastida
Authors: Liang Hongping , Wei Tong , Xu Yan , Li Linzhou , Kumar Sahu Sunil , Wang Hongli , Li Haoyuan , Fu Xian , Zhang Gengyun , Melkonian Michael , Liu Xin , Wang Sibo , Liu Huan
Published: June 2019

Selenoproteins that contain selenocysteine (Sec) are found in all kingdoms of life. Although they constitute a small proportion of the proteome, selenoproteins play essential roles in many organisms. In photosynthetic eukaryotes, selenoproteins have been found in algae but are missing in land plants (embryophytes). In this study, we explored the evolutionary dynamics of Sec incorporation by conveying a genomic search for the Sec machinery and selenoproteins across Archaeplastida. We identified a complete Sec machinery and variable sizes of selenoproteomes in the main algal lineages. However, the entire Sec machinery was missing in the Bangiophyceae-Florideophyceae clade (BV) of Rhodoplantae (red algae) and only partial machinery was found in three species of Archaeplastida, indicating parallel loss of Sec incorporation in different groups of algae. Further analysis of genome and transcriptome data suggests that all major lineages of streptophyte algae display a complete Sec machinery, although the number of selenoproteins is low in this group, especially in subaerial taxa. We conclude that selenoproteins tend to be lost in Archaeplastida upon adaptation to a subaerial or acidic environment. The high number of redox-active selenoproteins found in some bloom-forming marine microalgae may be related to defense against viral infections. Some of the selenoproteins in these organisms may have been gained by horizontal gene transfer from bacteria.
Strategies to Overcome Resistance Mechanisms in T-Cell Acute Lymphoblastic Leukemia
Authors: Follini Elena , Marchesini Matteo , Roti Giovanni
Published: June 2019

Chemoresistance is a major cause of recurrence and death from T-cell acute lymphoblastic leukemia (T-ALL), both in adult and pediatric patients. In the majority of cases, drug-resistant disease is treated by selecting a combination of other drugs, without understanding the molecular mechanisms by which malignant cells escape chemotherapeutic treatments, even though a more detailed genomic characterization and the identification of actionable disease targets may enable informed decision of new agents to improve patient outcomes. In this work, we describe pathways of resistance to common chemotherapeutic agents including glucocorticoids and review the resistance mechanisms to targeted therapy such as IL7R, PI3K-AKT-mTOR, NOTCH1, BRD4/MYC, Cyclin D3: CDK4/CDK6, BCL2 inhibitors, and selective inhibitors of nuclear export (SINE). Finally, to overcome the limitations of the current trial-and-error method, we summarize the experiences of anti-cancer drug sensitivity resistance profiling (DSRP) approaches as a rapid and relevant strategy to infer drug activity and provide functional information to assist clinical decision one patient at a time.
Managing Software Security Knowledge in Context: An Ontology Based Approach
Authors: Wen Shao-Fang , Katt Basel
Published: June 2019

Knowledge of software security is highly complex since it is quite context-specific and can be applied in diverse ways. To secure software development, software developers require not only knowledge about general security concepts but also about the context for which the software is being developed. With traditional security-centric knowledge formats, it is difficult for developers or knowledge users to retrieve their required security information based on the requirements of software products and development technologies. In order to effectively regulate the operation of security knowledge and be an essential part of practical software development practices, we argue that security knowledge must first incorporate features that specify what contextual characteristics are to be handled, and represent the security knowledge in a format that is understandable and acceptable to the individuals. This study introduces a novel ontology approach for modeling security knowledge with a context-based approach, by which security knowledge can be retrieved, taking the context of the software application at hand into consideration. In this paper, we present our security ontology with the design concepts and the corresponding evaluation process.
Computational Thinking and Down Syndrome: An Exploratory Study Using the KIBO Robot
Authors: González-González Carina S. , Herrera-González Erika , Moreno-Ruiz Lorenzo , Reyes-Alonso Nuria , Hernández-Morales Selene , Guzmán-Franco María D. , Infante-Moro Alfonso
Published: June 2019

Computational thinking and coding are key competencies in the 21st century. People with Down syndrome need to be part of this new literacy. For this reason, in this work, we present an exploratory study carried out with students with Down syndrome with cognitive ages of 3–6 years old using a tangible robot We applied the observational method during the sessions to analyze the participants’ emotional states, engagement, and comprehension of the programming sequences. Results show that people with cognitive disabilities can acquire basic programming and computational skills using tangible robots such as KIBO.