bluuebunny/crossref_metadata_2025 huggingface dataset:
One-Layer vs. Two-Layer SOM in the Context of Outlier Identification: A Simulation Study
Authors: Valverde Castilla Gabriel Antonio , Mira McWilliams José Manuel , González-Pérez Beatriz
Published: July 2021

In this work, we applied a stochastic simulation methodology to quantify the power of the detection of outlying mixture components of a stochastic model, when applying a reduced-dimension clustering technique such as Self-Organizing Maps (SOMs). The essential feature of SOMs, besides dimensional reduction into a discrete map, is the conservation of topology. In SOMs, two forms of learning are applied: competitive, by sequential allocation of sample observations to a winning node in the map, and cooperative, by the update of the weights of the winning node and its neighbors. By means of cooperative learning, the conservation of topology from the original data space to the reduced (typically 2D) map is achieved. Here, we compared the performance of one- and two-layer SOMs in the outlier representation task. The same stratified sampling was applied for both the one-layer and two-layer SOMs; although, stratification would only be relevant for the two-layer setting—to estimate the outlying mixture component detection power. Two distance measures between points in the map were defined to quantify the conservation of topology. The results of the experiment showed that the two-layer setting was more efficient in outlier detection while maintaining the basic properties of the SOM, which included adequately representing distances from the outlier component to the remaining ones.
A Comprehensive Review on Integration Challenges, Optimization Techniques and Control Strategies of Hybrid AC/DC Microgrid
Authors: Azeem Omar , Ali Mujtaba , Abbas Ghulam , Uzair Muhammad , Qahmash Ayman , Algarni Abdulmohsen , Hussain Mohammad Rashid
Published: July 2021

The depletion of natural resources and the intermittence of renewable energy resources have pressed the need for a hybrid microgrid, combining the benefits of both AC and DC microgrids, minimizing the overall deficiency shortcomings and increasing the reliability of the system. The hybrid microgrid also supports the decentralized grid control structure, aligning with the current scattered and concentrated load scenarios. Hence, there is an increasing need to explore and reveal the integration, optimization, and control strategies regarding the hybrid microgrid. A comprehensive study of hybrid microgrid’s performance parameters, efficiency, reliability, security, design flexibility, and cost-effectiveness is required. This paper discusses major issues regarding the hybrid microgrids, the integration of AC and DC microgrids, their security and reliability, the optimization of power generation and load management in different scenarios, the efficient management regarding uncertainty for renewable energy resources, the optimal placement of feeders, and the cost-effective control methodologies for the hybrid microgrid. The major research areas are briefly explained, aiming to find the research gap that can further improve the performance of the grid. In light of the recent trends in research, novel strategies are proposed that are found most effective and cost-friendly regarding the hybrid microgrid. This paper will serve as a baseline for future research, comparative analysis, and further development of novel techniques regarding hybrid microgrids.
Balancer Genetic Algorithm—A Novel Task Scheduling Optimization Approach in Cloud Computing
Authors: Gulbaz Rohail , Siddiqui Abdul Basit , Anjum Nadeem , Alotaibi Abdullah Alhumaidi , Althobaiti Turke , Ramzan Naeem
Published: July 2021

Task scheduling is one of the core issues in cloud computing. Tasks are heterogeneous, and they have intensive computational requirements. Tasks need to be scheduled on Virtual Machines (VMs), which are resources in a cloud environment. Due to the immensity of search space for possible mappings of tasks to VMs, meta-heuristics are introduced for task scheduling. In scheduling makespan and load balancing, Quality of Service (QoS) parameters are crucial. This research contributes a novel load balancing scheduler, namely Balancer Genetic Algorithm (BGA), which is presented to improve makespan and load balancing. Insufficient load balancing can cause an overhead of utilization of resources, as some of the resources remain idle. BGA inculcates a load balancing mechanism, where the actual load in terms of million instructions assigned to VMs is considered. A need to opt for multi-objective optimization for improvement in load balancing and makespan is also emphasized. Skewed, normal and uniform distributions of workload and different batch sizes are used in experimentation. BGA has exhibited significant improvement compared with various state-of-the-art approaches for makespan, throughput and load balancing.
Frequency Division Multiplexing of Terahertz Waves Realized by Diffractive Optical Elements
Authors: Komorowski Paweł , Czerwińska Patrycja , Kaluza Mateusz , Surma Mateusz , Zagrajek Przemysław , Sobczyk Artur , Ciurapiński Wiesław , Piramidowicz Ryszard , Siemion Agnieszka
Published: July 2021

Recently, one of the most commonly discussed applications of terahertz radiation is wireless telecommunication. It is believed that the future 6G systems will utilize this frequency range. Although the exact technology of future telecommunication systems is not yet known, it is certain that methods for increasing their bandwidth should be investigated in advance. In this paper, we present the diffractive optical elements for the frequency division multiplexing of terahertz waves. The structures have been designed as a combination of a binary phase grating and a converging diffractive lens. The grating allows for differentiating the frequencies, while the lens assures separation and focusing at the finite distance. Designed structures have been manufactured from polyamide PA12 using the SLS 3D printer and verified experimentally. Simulations and experimental results are shown for different focal lengths. Moreover, parallel data transmission is shown for two channels of different carrier frequencies propagating in the same optical path. The designed structure allowed for detecting both signals independently without observable crosstalk. The proposed diffractive elements can work in a wide range of terahertz and sub-terahertz frequencies, depending on the design assumptions. Therefore, they can be considered as an appealing solution, regardless of the band finally used by the future telecommunication systems.
Cavitator Design for Straight-Running Supercavitating Torpedoes
Authors: Kim Min-Jae , Kim Seon-Hong , Lee Kurn-Chul , Paik Bu-Geun , Kim Moon-Chan
Published: July 2021

A practical cavitator design method for straight-running-type supercavitating torpedoes was developed in this paper. Design requirements were first drawn in terms of torpedo performance characteristics, such as maximum range and motion stability. This method determines the optimum cavitator satisfying the design requirements that not only minimize the total drag of the torpedo, extending the maximum range, but also provide hydrodynamic forces required for straight level flight. The design procedure includes determining a design cavitation number and cavitator type (disk or cone) for obtaining the optimal cavitator that minimizes the total drag of a torpedo in straight level flight. To determine such an optimal cavitator, the equations of force and moment equilibrium for straight level flight were iteratively solved by the existing mathematical models that determine the cavity shapes generated by disk- and cone-shaped cavitators and hydrodynamic forces acting on the vehicle. For validation, model experiments on a small-scale supercavitating vehicle were conducted in a towing tank, and the results agree well with those of the mathematical models used in this study. A preliminary design based on the newly proposed method was also implemented for a realistic supercavitating vehicle. More precise computations using CFD should be conducted to investigate the physics in more detail in the near future.
Special Issue: Musculoskeletal Models in a Clinical Perspective
Authors: Frigo Carlo Albino
Published: July 2021

After the pioneering work of Scott Delp and colleagues dated 1990 (An interactive graphics-based model of the lower extremity to study orthopaedic surgical procedures, [...]
Method of Multilevel Adaptive Synthesis of Monitoring Object Knowledge Graphs
Authors: Krinkin Kirill , Vodyaho Alexander , Kulikov Igor , Zhukova Nataly
Published: July 2021

The paper introduces a method for adaptive deductive synthesis of state models, of complex objects, with multilevel variable structures. The method makes it possible to predict the state of objects using the data coming from them. The data from the objects are collected with sensors installed on them. Multilevel knowledge graphs (KG) are used to describe the observed objects. The new adaptive synthesis method develops previously proposed inductive and deductive synthesis methods, allowing the context to be taken into account when predicting the states of the monitored objects based on the data obtained from them. The article proposes the algorithm for the suggested method and presents its computational complexity analysis. The software system, based on the proposed method, and the algorithm for multilevel adaptive synthesis of the object models developed, are described in the article. The effectiveness of the proposed method is shown in the results from modeling the states of telecommunication networks of cable television operators.
Research and Applied Perspective to Blockchain Technology: A Comprehensive Survey
Authors: Johar Sumaira , Ahmad Naveed , Asher Warda , Cruickshank Haitham , Durrani Amad
Published: July 2021

Blockchain being a leading technology in the 21st century is revolutionizing each sector of life. Services are being provided and upgraded using its salient features and fruitful characteristics. Businesses are being enhanced by using this technology. Countries are shifting towards digital currencies i.e., an initial application of blockchain application. It omits the need of central authority by its distributed ledger functionality. This distributed ledger is achieved by using a consensus mechanism in blockchain. A consensus algorithm plays a core role in the implementation of blockchain. Any application implementing blockchain uses consensus algorithms to achieve its desired task. In this paper, we focus on provisioning of a comparative analysis of blockchain’s consensus algorithms with respect to the type of application. Furthermore, we discuss the development platforms as well as technologies of blockchain. The aim of the paper is to provide knowledge from basic to extensive from blockchain architecture to consensus methods, from applications to development platform, from challenges and issues to blockchain research gaps in various areas.
Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods
Authors: Slama Houda Ben , Chenari Bouket Ali , Pourhassan Zeinab , Alenezi Faizah N. , Silini Allaoua , Cherif-Silini Hafsa , Oszako Tomasz , Luptakova Lenka , Golińska Patrycja , Belbahri Lassaad
Published: July 2021

Natural dyes have been used from ancient times for multiple purposes, most importantly in the field of textile dying. The increasing demand and excessive costs of natural dye extraction engendered the discovery of synthetic dyes from petrochemical compounds. Nowadays, they are dominating the textile market, with nearly 8 × 105 tons produced per year due to their wide range of color pigments and consistent coloration. Textile industries consume huge amounts of water in the dyeing processes, making it hard to treat the enormous quantities of this hazardous wastewater. Thus, they have harmful impacts when discharged in non-treated or partially treated forms in the environment (air, soil, plants and water), causing several human diseases. In the present work we focused on synthetic dyes. We started by studying their classification which depended on the nature of the manufactured fiber (cellulose, protein and synthetic fiber dyes). Then, we mentioned the characteristics of synthetic dyes, however, we focused more on their negative impacts on the ecosystem (soil, plants, water and air) and on humans. Lastly, we discussed the applied physical, chemical and biological strategies solely or in combination for textile dye wastewater treatments. Additionally, we described the newly established nanotechnology which achieves complete discharge decontamination.
Tele-Rehabilitation Versus Local Rehabilitation Therapies Assisted by Robotic Devices: A Pilot Study with Patients
Authors: Catalán José M. , García-Pérez José V. , Blanco Andrea , Ezquerro Santiago , Garrote Alicia , Costa Teresa , Bertomeu-Motos Arturo , Díaz Iñaki , García-Aracil Nicolás
Published: July 2021

The present study aims to evaluate the advantages of a master-slave robotic rehabilitation therapy in which the patient is assisted in real-time by a therapist. We have also explored if this type of strategy is applicable in a tele-rehabilitation environment. A pilot study has been carried out involving 10 patients who have performed a point-to-point rehabilitation exercise supported by three assistance modalities: fixed assistance (without therapist interaction), local therapist assistance, and remote therapist assistance in a simulated tele-rehabiliation scenario. The rehabilitation exercise will be performed using an upper-limb rehabilitation robotic device that assists the patients through force fields. The results suggest that the assistance provided by the therapist is better adapted to patient needs than fixed assistance mode. Therefore, it maximizes the patient’s level of effort, which is an important aspect to improve the rehabilitation outcomes. We have also seen that in a tele-rehabilitation environment it is more difficult to assess when to assist the patient than locally. However, the assistance suits patients better than the fixed assistance mode.
Study on Two-Phase Mixing Inside Flow Focusing/Blurring Nozzle by Gray Distribution Analysis Method
Authors: Zhao Jin , Ning Zhi , Lü Ming , Sun Chunhua
Published: July 2021

The application prospect of a flow focusing/blurring nozzle is broad but research on gas-liquid flow inside the nozzle is not comprehensive. The gas-liquid mixing inside the nozzle is difficult to study by visualization experiment, so this paper proposes to study the gas-liquid flow or mixing inside the nozzle by the gray scale level distribution of the experimental images. The results show that the increase of air flow rate is beneficial to two-phase mixing inside the nozzle, while the influence of water flow rate, tube hole distance (the distance between inner tube and nozzle outlet) and orifice diameter increase is opposite. The influence of air inertia force on two-phase mixing is weaker than the water inertia force under different parameters, the effect of the air inertia force on two-phase mixing is similar to tube hole distance under a small flow rate, the effect of the orifice diameter on two-phase mixing is relatively weak. In addition, the analysis of the gas-liquid flow field in the mixing zone shows that the gas-liquid flow in the nozzle is stable in the flow focusing mode. In the flow blurring mode, the gas-liquid flow inside the nozzle has radial stability but axial pulsation. In the transition mode, the gas-liquid flow inside the nozzle is unstable, but the gas-liquid flow is close to the flow blurring mode.
A Study on COVID-19 Incidence in Europe through Two SEIR Epidemic Models Which Consider Mixed Contagions from Asymptomatic and Symptomatic Individuals
Authors: Nistal Raúl , de la Sen Manuel , Gabirondo Jon , Alonso-Quesada Santiago , Garrido Aitor J. , Garrido Izaskun
Published: July 2021

The impact of the SARS-CoV-2 (COVID-19) on the world has been partially controlled through different measures of social isolation and prophylaxis. Two new SEIR (Susceptible-Exposed-Infected-Recovered) models are proposed in order to describe this spread through different countries of Europe. In both models the infectivity of the asymptomatic period during the exposed stage of the disease will be taken into account. The different transmission rates of the SEIR models are calculated by considering the different locations and, more importantly, the lockdown measures implemented in each region. A new classification of these intervention measures will be set and their influence on the values of the transmission rates will be estimated through regression analysis.
Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients
Authors: Bishr Amr S. , Abdelaziz Salma M. , Yahia Ibrahim S. , Yassien Mahmoud A. , Hassouna Nadia A. , Aboshanab Khaled M.
Published: July 2021

Macrolide-resistant methicillin-resistant Staphylococcus aureus (MAC-MRSA) is one of the most clinically relevant pathogens due to its significant ability of resistance acquisition to different antimicrobial agents. This study aimed to evaluate antimicrobial susceptibility and the use of different combinations of azithromycin with other antibiotics for combating MAC resistance. Seventy-two Staphylococci (38.5%) (n = 187), showed resistance to MACs; of these, 53 isolates (73.6%, n = 72) were S. aureus and 19 (26.4%, n = 72) were coagulase-negative staphylococci (CoNS). Out of the 53 S. aureus and 19 CoNS isolates, 38 (71.7%, n = 53) and 9 (47.4%, n = 19) were MRSA and methicillin-resistant CoNS, respectively. The constitutive MACs, lincosamides and streptogramin-B (cMLS) comprised the predominant phenotype among S. aureus isolates (54.7%) and CoNS isolates (78.9%). The PCR analysis showed that the ermC gene was the most prevalent (79.2%), followed by msrA (48.6%), and ermA (31.9%). Azithromycin combinations with either linezolid, ceftriaxone, gentamicin, or cefotaxime provided synergy in 42.1%, 44.7%, 31.6% and 7.9% of the 38 MAC-MRSA isolates, respectively. Statistical analysis showed significant association between certain MAC resistance genotypes and the synergistic effect of certain azithromycin combinations (p value < 0.05). In conclusion, azithromycin combinations with either linezolid, or ceftriaxone showed synergism in most of the MAC-resistant MRSA clinical isolates.
Ozone Gel in Chronic Periodontal Disease: A Randomized Clinical Trial on the Anti-Inflammatory Effects of Ozone Application
Authors: Colombo Marco , Gallo Simone , Garofoli Alessandro , Poggio Claudio , Arciola Carla Renata , Scribante Andrea
Published: July 2021

The search for new topical treatments able to display not only antimicrobial properties but also a multiplicity of other beneficial effects while expressing safe cytocompatibility toward host tissues is being progressively developed. Antiseptics represent an aid to the gold standard nonsurgical treatment Scaling-and-Root-Planing (SRP) for periodontal disease. This split-mouth study aims to assess the efficacy of the ozonized gel GeliO3 (Bioemmei Srl, Vicenza, Italy) plus SRP (experimental treatment), with respect to SRP + chlorhexidine gel. Ten participants were treated with SRP + chlorhexidine gel (control sites) and with SRP + ozone gel (trial sites). After 1 (T1) and 3 months (T2) from baseline (T0), patients were revisited. At each time-point, the following indexes were assessed: probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI), plaque index (PI), and bleeding on probing (BoP). It has been assessed that the use of the ozonized gel in addition to SRP did not show significant differences if compared to conventional SRP + chlorhexidine. Chlorhexidine was found to be more effective than ozone in reducing CAL and GI at T2. Ozone deserves consideration for its wide applicability in several clinical fields. In this connection, we also glance at the latest research on ozone therapy.
Revealing New Landscape of Turbot (Scophthalmus maximus) Spleen Infected with Aeromonas salmonicida through Immune Related circRNA-miRNA-mRNA Axis
Authors: Xue Ting , Liu Yiping , Cao Min , Tian Mengyu , Zhang Lu , Wang Beibei , Liu Xiaoli , Li Chao
Published: July 2021

Increasing evidence suggests that non-coding RNAs (ncRNA) play an important role in a variety of biological life processes by regulating gene expression at the transcriptional and post-transcriptional levels. Turbot (Scophthalmus maximus) has been threatened by various pathogens. In this study, the expression of circular RNAs (circRNAs), microRNAs (miRNAs), and mRNA in the immune organs spleen of turbot infected with Aeromonas salmonicida was analyzed by high-throughput sequencing, and a circRNA-miRNA-mRNA network was constructed, so as to explore the function of non-coding RNA in the immune system of teleost. Illumina sequencing was performed on the uninfected group and infected group. A total of 119 differential expressed circRNAs (DE-circRNAs), 140 DE-miRNAs, and 510 DE-mRNAs were identified in the four infected groups compared with the uninfected group. Most DE-mRNAs and the target genes of DE-ncRNAs were involved in immune-related pathways. The quantitative real-time PCR (qRT-PCR) results verified the reliability and accuracy of the high-throughput sequencing data. Ninety-six differentially expressed circRNA-miRNA-mRNA regulatory networks were finally constructed. Among them, 15 circRNA-miRNA-mRNA were presented in the form of “up (circRNA)-down (miRNA)-up (mRNA)” or “down-up-down”. Immune-related genes gap junction CX32.2, cell adhesion molecule 3, and CC chemokine were also found in these networks. These results indicate that ncRNA may regulate the expression of immune-related genes through the circRNA-miRNA-mRNA regulatory network and thus participate in the immune response of turbot spleen after pathogen infection.
Efficiency of a Seedling Phenotyping Strategy to Support European Wheat Breeding Focusing on Leaf Rust Resistance
Authors: Beukert Ulrike , Pfeiffer Nina , Ebmeyer Erhard , Hinterberger Valentin , Lueck Stefanie , Serfling Albrecht , Ordon Frank , Schulthess Albert Wilhelm , Reif Jochen Christoph
Published: July 2021

Leaf rust resistance is of high importance for a sustainable European wheat production. The expression of known resistance genes starts at different developmental stages of wheat. Breeding for resistance can be supported by a fast, precise, and resource-saving phenotyping. The examination of detached leaf assays of juvenile plants inoculated under controlled conditions and phenotyped by a robotic- and computer-based, high-throughput system is a promising approach in this respect. Within this study, the validation of the phenotyping workflow was conducted based on a winter wheat set derived from Central Europe and examined at different plant developmental stages. Moderate Pearson correlations of 0.38–0.45 comparing leaf rust resistance of juvenile and adult plants were calculated and may be mainly due to different environmental conditions. Specially, the infection under controlled conditions was limited by the application of a single rust race at only one time point. Our results suggest that the diversification with respect to the applied rust race spectrum is promising to increase the consistency of detached leaf assays and the transferability of its results to the field.
VEGF as a Direct Functional Regulator of Photoreceptors and Contributing Factor to Diabetes-Induced Alteration of Photoreceptor Function
Authors: Hu Jianyan , Zhu Meili , Li Dai , Wu Qiang , Le Yun-Zheng
Published: July 2021

Vascular endothelial growth factor (VEGF) is a major therapeutic target for blood–retina barrier (BRB) breakdown in diabetic retinopathy (DR), age-related macular degeneration (AMD), and other hypoxic retinal vascular disorders. To determine whether VEGF is a direct regulator of retinal neuronal function and its potential role in altering vision during the progression of DR, we examined the immediate impact of recombinant VEGF (rVEGF) on photoreceptor function with electroretinography in C57BL6 background wild-type (WT) and Akita spontaneous diabetic mice. Shortly after intravitreal injections, rVEGF caused a significant reduction of scotopic ERG a-wave and b-wave amplitudes and photopic ERG b-wave amplitudes in a dose-dependent manner in dark-adapted 1.5-mo-old WT mice. Compared with WT controls, 5-mo-old Akita spontaneous diabetic mice demonstrated a significant reduction in scotopic ERG a-wave and b-wave amplitudes and photopic ERG b-wave amplitudes. However, the effect of rVEGF altered photoreceptor function in WT controls was diminished in 5-mo-old Akita spontaneous diabetic mice. In conclusion, our results suggest that VEGF is a direct functional regulator of photoreceptors and VEGF up-regulation in DR is a contributing factor to diabetes-induced alteration of photoreceptor function. This information is critical to the understanding of the therapeutic effect and to the care of anti-VEGF drug-treated patients for BRB breakdown in DR, AMD, and other hypoxic retinal vascular disorders.
The Mechanism of CD8+ T Cells for Reducing Myofibroblasts Accumulation during Renal Fibrosis
Authors: Gao Min , Wang Jing , Zang Jianghua , An Yina , Dong Yanjun
Published: July 2021

Renal fibrosis is a hallmark of chronic kidney disease (CKD) and a common manifestation of end-stage renal disease that is associated with multiple types of renal insults and functional loss of the kidney. Unresolved renal inflammation triggers fibrotic processes by promoting the activation and expansion of extracellular matrix-producing fibroblasts and myofibroblasts. Growing evidence now indicates that diverse T cells and macrophage subpopulations play central roles in the inflammatory microenvironment and fibrotic process. The present review aims to elucidate the role of CD8+ T cells in renal fibrosis, and identify its possible mechanisms in the inflammatory microenvironment.
Do We Store Packed Red Blood Cells under “Quasi-Diabetic” Conditions?
Authors: Livshits Leonid , Barshtein Gregory , Arbell Dan , Gural Alexander , Levin Carina , Guizouarn Hélène
Published: July 2021

Red blood cell (RBC) transfusion is one of the most common therapeutic procedures in modern medicine. Although frequently lifesaving, it often has deleterious side effects. RBC quality is one of the critical factors for transfusion efficacy and safety. The role of various factors in the cells’ ability to maintain their functionality during storage is widely discussed in professional literature. Thus, the extra- and intracellular factors inducing an accelerated RBC aging need to be identified and therapeutically modified. Despite the extensively studied in vivo effect of chronic hyperglycemia on RBC hemodynamic and metabolic properties, as well as on their lifespan, only limited attention has been directed at the high sugar concentration in RBCs storage media, a possible cause of damage to red blood cells. This mini-review aims to compare the biophysical and biochemical changes observed in the red blood cells during cold storage and in patients with non-insulin-dependent diabetes mellitus (NIDDM). Given the well-described corresponding RBC alterations in NIDDM and during cold storage, we may regard the stored (especially long-stored) RBCs as “quasi-diabetic”. Keeping in mind that these RBC modifications may be crucial for the initial steps of microvascular pathogenesis, suitable preventive care for the transfused patients should be considered. We hope that our hypothesis will stimulate targeted experimental research to establish a relationship between a high sugar concentration in a storage medium and a deterioration in cells’ functional properties during storage.
The Oscillatory Profile Induced by the Anxiogenic Drug FG-7142 in the Amygdala–Hippocampal Network Is Reversed by Infralimbic Deep Brain Stimulation: Relevance for Mood Disorders
Authors: Vila-Merkle Hanna , González-Martínez Alicia , Campos-Jiménez Rut , Martínez-Ricós Joana , Teruel-Martí Vicent , Blasco-Serra Arantxa , Lloret Ana , Celada Pau , Cervera-Ferri Ana
Published: July 2021

Anxiety and depression exhibit high comorbidity and share the alteration of the amygdala–hippocampal–prefrontal network, playing different roles in the ventral and dorsal hippocampi. Deep brain stimulation of the infralimbic cortex in rodents or the human equivalent—the subgenual cingulate cortex—constitutes a fast antidepressant treatment. The aim of this work was: (1) to describe the oscillatory profile in a rodent model of anxiety, and (2) to deepen the therapeutic basis of infralimbic deep brain stimulation in mood disorders. First, the anxiogenic drug FG-7142 was administered to anaesthetized rats to characterize neural oscillations within the amygdala and the dorsoventral axis of the hippocampus. Next, deep brain stimulation was applied. FG-7142 administration drastically reduced the slow waves, increasing delta, low theta, and beta oscillations in the network. Moreover, FG-7142 altered communication in these bands in selective subnetworks. Deep brain stimulation of the infralimbic cortex reversed most of these FG-7142 effects. Cross-frequency coupling was also inversely modified by FG-7142 and by deep brain stimulation. Our study demonstrates that the hyperactivated amygdala–hippocampal network associated with the anxiogenic drug exhibits an oscillatory fingerprint. The study contributes to comprehending the neurobiological basis of anxiety and the effects of infralimbic deep brain stimulation.
The Automatization of a New Thermography Method Using Invasive Nociceptive Stimulation to Confirm an Autonomic Phenomenon within a Trigger Point Referred Pain Zone
Authors: Skorupska Elżbieta , Dybek Tomasz , Rychlik Michał , Jokiel Marta , Dobrakowski Paweł
Published: July 2021

The trigger points (TrPs) related to chronic low back pain that mimic sciatica have been lately recognized and included in the International Classification of Diseases, 11th Revision. This study examined the MATLAB software utility for the objective stratification of low back pain patients using the Minimally Invasive Procedure (MIP). The two diagnostic MIP parameters were: average temperature (ΔTavr) and autonomic referred pain (AURP). Chronic sciatica patients with TrPs (n = 20) and without TrPs (n = 20) were examined using the MIP. A significant increase in both parameters was confirmed for the thigh ROI of the TrP-positive patients, with ΔTavr being the leading parameter (p = 0.016, Exp(β) = 2.603). A continued significance of both parameters was confirmed from 6′00″ to 15′30″ (p < 0.05). The maximum AURP value was confirmed at 13′30″ (p < 0.05) (TrPs(+) 20.4 ± 19.9% vs. TrPs(-) 3.77 ± 9.14%; p = 0.000; CI (0.347,0.348)).
Prospects of Developing Prefabricated Masonry Walling Systems in Australia
Authors: Thamboo Julian , Zahra Tatheer , Navaratnam Satheeskumar , Asad Mohammad , Poologanathan Keerthan
Published: July 2021

Prefabrication has been shown to be an effective way of construction in the modern-day context. Although much progress has been made in developing reinforced concrete (RC), timber and steel prefabricated elements/structures, prefabrication of masonry walling systems has received limited attention in the past. Conventional masonry construction is labour-intensive and time-consuming; therefore, prefabrication can be an effective solution to accelerate the masonry construction to make it more cost-effective. Therefore, in this paper, an attempt has been made to evaluate the effectiveness of prefabricated masonry systems (PMS) in terms of their structural characteristics and sustainability perspectives in an Australian context. Subsequently, the available studies related to PMS and the prospects of developing prefabricated masonry walling systems were appraised and reported. In order to assess the applicability of PMS, a case study was carried out by designing four types of prospective prefabricated masonry walling systems for a typical housing unit in Australia. It was shown that the reinforced (RM), post-tensioned (PT) and thin layered mortared (TLM) masonry systems are better suited for prefabrication. Later, in order to assess the sustainability of the considered masonry walling systems, life cycle energy analyses were carried using the Environmental Performance in Construction (EPIC) database. It was found that there can be nearly 30% and 15% savings, respectively, in terms of energy saving and CO2 emissions in prefabricated construction than the conventional masonry construction. Finally, the prospects of developing PMS and the need for future research studies on these systems are highlighted.
Displacement-Based Seismic Assessment of the Likelihood of Failure of Reinforced Concrete Wall Buildings
Authors: Orumiyehei Amirhossein , Sullivan Timothy J.
Published: July 2021

To strengthen the resilience of our built environment, a good understanding of seismic risk is required. Probabilistic performance-based assessment is able to rigorously compute seismic risk and the advent of numerical computer-based analyses has helped with this. However, it is still a challenging process and as such, this study presents a simplified probabilistic displacement-based assessment approach for reinforced concrete wall buildings. The proposed approach is trialed by applying the methodology to 4-, 8-, and 12-story case study buildings, and results are compared with those obtained via multi-stripe analyses, with allowance for uncertainty in demand and capacity, including some allowance for modeling uncertainty. The results indicate that the proposed approach enables practitioners to practically estimate the median intensity associated with exceeding a given mechanism and the annual probability of exceeding assessment limit states. Further research to extend the simplified approach to other structural systems is recommended. Moreover, the research highlights the need for more information on the uncertainty in our strength and deformation estimates, to improve the accuracy of risk assessment procedures.
Surgical Skills and Technological Advancements to Avoid Complications in Lateral Neck Dissection for Differentiated Thyroid Cancer
Authors: Bove Aldo , Farrukh Maira , Di Gioia Adele , Di Resta Velia , Buffone Angelica , Melchionna Claudia , Panaccio Paolo
Published: July 2021

Neck dissection is a surgical procedure reserved for thyroid cancer cases with clinically evident lymphatic invasion. Although neck dissection is a reliable and safe procedure, it can determine a significant morbidity involving a variety of structures of nervous, vascular and endocrine typology. A careful pre-operative study is therefore essential to better plan surgery. Surgical experience, combined with accurate surgical preparation and merged with adequate and specific techniques, can certainly help reduce the percentage of complications. In recent years, however, technology has also proved to be useful. Its crucial role was already recognized in the safeguard of the integrity of the laryngeal nerve through neuro-monitoring, but new technologies are emerging to help the preservation also of the parathyroid glands and other structures, such as the thoracic duct. These surgical skills combined with the latest technological advancements, that allow us to reduce the incidence of complications after neck dissection for thyroid cancer, will be reported in the present article. This topic is of significant interest for the endocrine and metabolic surgeons’ community.
Two-Step Esterification–Hydrogenation of Bio-Oil to Alcohols and Esters over Raney Ni Catalysts
Authors: Xu Ying , Zhang Limin , Lv Wei , Wang Chenguang , Wang Congwei , Zhang Xinghua , Zhang Qi , Ma Longlong
Published: July 2021

Fast pyrolysis bio-oil is very difficult to be used because of its acidity, instability, high degree of unsaturation, etc. Processes for property upgrading are necessary and required. In this study, three kinds of Raney Ni catalysts were prepared and used to investigate two-step esterification–hydrogenation (TEH) to upgrade the light fraction of bio-oil. The results show that the first step in esterification markedly decreased the content of active compounds such as acids and ketones and aldehydes and increased the content of alcohols and esters (from 10.53% to 47.55%), which improved the bio-oil stability and was favorable for the following hydrogenation reaction. The second step of TEH (hydrogenation) further improved the quality of the bio-oil over Raney Ni and metal-modified Raney Ni catalysts at 140 °C. In particular, the Mo-RN catalyst displayed the best hydrogenation effect, with only 5.44% of acid content, and the stable component content reached 90.16%. This may be attributed to the higher hydrogenation activity from Raney Ni combined with acid MoOx species and the thermal stability of the catalyst. Moreover, the obtained upgraded bio-oil mixture could be used as a solvent for raw bio-oil’s esterification. Therefore, it has the potential to reduce methanol solvent usage and energy consumption for solvent separation during the two-step treatment of raw bio-oil in this context. Compared with the OHE (one-step esterification-hydrogenation) process, THE showed a better performance for raw bio-oil upgrading with higher alcohols and stable compounds, which is more favorable for the saturation and stability of bio-oil’s complex components step by step.
A Simple and Straightforward Method for Activity Measurement of Carbonic Anhydrases
Authors: Fuchs Werner , Steger Franziska , Reich Johanna , Ribitsch Doris , Rittmann Simon K.-M. R. , Bochmann Günther
Published: July 2021

Carbonic anhydrase (CA) is an enzyme of high interest due to its high implications relative to the medical and environmental sectors. In the current paper, an enzyme assay for the determination of CA activity is proposed and it is characterized by its simplicity and high practicability. It permits the straightforward comparison of CAs performance in physiological conditions. The methodology and the theoretical background of the evaluation method are explained in detail. Moreover, the presumed advantages over alternative assays are discussed. The assay has proven to be particularly useful for the screening of CA activity with respect to their application in CO2, capturing processes for further utilization or storage.
The Catalytic Activity of Biosynthesized Magnesium Oxide Nanoparticles (MgO-NPs) for Inhibiting the Growth of Pathogenic Microbes, Tanning Effluent Treatment, and Chromium Ion Removal
Authors: Saied Ebrahim , Eid Ahmed , Hassan Saad , Salem Salem , Radwan Ahmed , Halawa Mahmoud , Saleh Fayez , Saad Hosam , Saied Essa , Fouda Amr
Published: July 2021

Magnesium oxide nanoparticles (MgO-NPs) were synthesized using the fungal strain Aspergillus terreus S1 to overcome the disadvantages of chemical and physical methods. The factors affecting the biosynthesis process were optimized as follows: concentration of Mg(NO3)2·6H2O precursor (3 mM), contact time (36 min), pH (8), and incubation temperature (35 °C). The characterization of biosynthesized MgO-NPs was accomplished using UV-vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy—energy dispersive X-ray (SEM-EDX), X-ray diffraction (XRD), and dynamic light scattering (DLS). Data confirmed the successful formation of crystallographic, spherical, well-dispersed MgO-NPs with a size range of 8.0–38.0 nm at a maximum surface plasmon resonance of 280 nm. The biological activities of biosynthesized MgO-NPs including antimicrobial activity, biotreatment of tanning effluent, and chromium ion removal were investigated. The highest growth inhibition of pathogenic Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans was achieved at 200 μg mL–1 of MgO-NPs. The biosynthesized MgO-NPs exhibited high efficacy to decolorize the tanning effluent (96.8 ± 1.7% after 150 min at 1.0 µg mL–1) and greatly decrease chemical parameters including total suspended solids (TSS), total dissolved solids (TDS), biological oxygen demand (BOD), chemical oxygen demand (COD), and conductivity with percentages of 98.04, 98.3, 89.1, 97.2, and 97.7%, respectively. Further, the biosynthesized MgO-NPs showed a strong potential to remove chromium ions from the tanning effluent, from 835.3 mg L–1 to 21.0 mg L–1, with a removal percentage of 97.5%.
Tuning IgE: IgE-Associating Molecules and Their Effects on IgE-Dependent Mast Cell Reactions
Authors: Ando Tomoaki , Kitaura Jiro
Published: July 2021

The recent emergence of anti-immunoglobulin E (IgE) drugs and their candidates for humans has endorsed the significance of IgE-dependent pathways in allergic disorders. IgE is distributed locally in the tissues or systemically to confer a sensory mechanism in a domain of adaptive immunity to the otherwise innate type of effector cells, namely, mast cells and basophils. Bound on the high-affinity IgE receptor FcεRI, IgE enables fast memory responses against revisiting threats of venoms, parasites, and bacteria. However, the dysregulation of IgE-dependent reactions leads to potentially life-threatening allergic diseases, such as asthma and anaphylaxis. Therefore, reactivity of the IgE sensor is fine-tuned by various IgE-associating molecules. In this review, we discuss the mechanistic basis for how IgE-dependent mast cell activation is regulated by the IgE-associating molecules, including the newly developed therapeutic candidates.
A Bimodal Fluorescence-Raman Probe for Cellular Imaging
Authors: Lin Jiarun , Graziotto Marcus E. , Lay Peter A. , New Elizabeth J.
Published: July 2021

Biochemical changes in specific organelles underpin cellular function, and studying these changes is crucial to understand health and disease. Fluorescent probes have become important biosensing and imaging tools as they can be targeted to specific organelles and can detect changes in their chemical environment. However, the sensing capacity of fluorescent probes is highly specific and is often limited to a single analyte of interest. A novel approach to imaging organelles is to combine fluorescent sensors with vibrational spectroscopic imaging techniques; the latter provides a comprehensive map of the relative biochemical distributions throughout the cell to gain a more complete picture of the biochemistry of organelles. We have developed NpCN1, a bimodal fluorescence-Raman probe targeted to the lipid droplets, incorporating a nitrile as a Raman tag. NpCN1 was successfully used to image lipid droplets in 3T3-L1 cells in both fluorescence and Raman modalities, reporting on the chemical composition and distribution of the lipid droplets in the cells.
Phosphorylation in the Charged Linker Modulates Interactions and Secretion of Hsp90β
Authors: Weidenauer Lorenz , Quadroni Manfredo
Published: July 2021

Hsp90β is a major chaperone involved in numerous cellular processes. Hundreds of client proteins depend on Hsp90β for proper folding and/or activity. Regulation of Hsp90β is critical to coordinate its tasks and is mediated by several post-translational modifications. Here, we focus on two phosphorylation sites located in the charged linker region of human Hsp90β, Ser226 and Ser255, which have been frequently reported but whose function remains unclear. Targeted measurements by mass spectrometry indicated that intracellular Hsp90β is highly phosphorylated on both sites (>90%). The level of phosphorylation was unaffected by various stresses (e.g., heat shock, inhibition with drugs) that impact Hsp90β activity. Mutating the two serines to alanines increased the amount of proteins interacting with Hsp90β globally and increased the sensitivity to tryptic cleavage in the C-terminal domain. Further investigation revealed that phosphorylation on Ser255 and to a lesser extent on Ser226 is decreased in the conditioned medium of cultured K562 cells, and that a non-phosphorylatable double alanine mutant was secreted more efficiently than the wild type. Overall, our results show that phosphorylation events in the charged linker regulate both the interactions of Hsp90β and its secretion, through changes in the conformation of the chaperone.
The Role of miRNAs in Extracellular Matrix Repair and Chronic Fibrotic Lung Diseases
Authors: Usman Kauna , Hsieh Aileen , Hackett Tillie-Louise
Published: July 2021

The lung extracellular matrix (ECM) plays a key role in the normal architecture of the lung, from embryonic lung development to mechanical stability and elastic recoil of the breathing adult lung. The lung ECM can modulate the biophysical environment of cells through ECM stiffness, porosity, topography and insolubility. In a reciprocal interaction, lung ECM dynamics result from the synthesis, degradation and organization of ECM components by the surrounding structural and immune cells. Repeated lung injury and repair can trigger a vicious cycle of aberrant ECM protein deposition, accompanied by elevated ECM stiffness, which has a lasting effect on cell and tissue function. The processes governing the resolution of injury repair are regulated by several pathways; however, in chronic lung diseases such as asthma, chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary disease (IPF) these processes are compromised, resulting in impaired cell function and ECM remodeling. Current estimates show that more than 60% of the human coding transcripts are regulated by miRNAs. miRNAs are small non-coding RNAs that regulate gene expressions and modulate cellular functions. This review is focused on the current knowledge of miRNAs in regulating ECM synthesis, degradation and topography by cells and their dysregulation in asthma, COPD and IPF.
Human Tissue Angiotensin Converting Enzyme (ACE) Activity Is Regulated by Genetic Polymorphisms, Posttranslational Modifications, Endogenous Inhibitors and Secretion in the Serum, Lungs and Heart
Authors: Bánhegyi Viktor , Enyedi Attila , Fülöp Gábor Áron , Oláh Attila , Siket Ivetta Mányiné , Váradi Csongor , Bottyán Klaudia , Lódi Mária , Csongrádi Alexandra , Umar Azeem J. , Fagyas Miklós , Czuriga Dániel , Édes István , Pólos Miklós , Merkely Béla , Csanádi Zoltán , Papp Zoltán , Szabó Gábor , Radovits Tamás , Takács István , Tóth Attila
Published: July 2021

Objective: Inhibitors of the angiotensin converting enzyme (ACE) are the primarily chosen drugs to treat heart failure and hypertension. Moreover, an imbalance in tissue ACE/ACE2 activity is implicated in COVID-19. In the present study, we tested the relationships between circulating and tissue (lung and heart) ACE levels in men. Methods: Serum, lung (n = 91) and heart (n = 72) tissue samples were collected from Caucasian patients undergoing lung surgery or heart transplantation. ACE I/D genotype, ACE concentration and ACE activity were determined from serum and tissue samples. Clinical parameters were also recorded. Results: A protocol for ACE extraction was developed for tissue ACE measurements. Extraction of tissue-localized ACE was optimal in a 0.3% Triton-X-100 containing buffer, resulting in 260 ± 12% higher ACE activity over detergent-free conditions. SDS or higher Triton-X-100 concentrations inhibited the ACE activity. Serum ACE concentration correlated with ACE I/D genotype (II: 166 ± 143 ng/mL, n = 19, ID: 198 ± 113 ng/mL, n = 44 and DD: 258 ± 109 ng/mL, n = 28, p < 0.05) as expected. In contrast, ACE expression levels in the lung tissue were approximately the same irrespective of the ACE I/D genotype (II: 1423 ± 1276 ng/mg, ID: 1040 ± 712 ng/mg and DD: 930 ± 1273 ng/mg, p > 0.05) in the same patients (values are in median ± IQR). Moreover, no correlations were found between circulating and lung tissue ACE concentrations and activities (Spearman’s p > 0.05). In contrast, a significant correlation was identified between ACE activities in serum and heart tissues (Spearman’s Rho = 0.32, p < 0.01). Finally, ACE activities in lung and the serum were endogenously inhibited to similar degrees (i.e., to 69 ± 1% and 53 ± 2%, respectively). Conclusion: Our data suggest that circulating ACE activity correlates with left ventricular ACE, but not with lung ACE in human. More specifically, ACE activity is tightly coordinated by genotype-dependent expression, endogenous inhibition and secretion mechanisms.