bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice
Authors: Cuffaro Bernardo , Assohoun Aka L. W. , Boutillier Denise , Súkeníková Lenka , Desramaut Jérémy , Boudebbouze Samira , Salomé-Desnoulez Sophie , Hrdý Jiří , Waligora-Dupriet Anne-Judith , Maguin Emmanuelle , Grangette Corinne
Published: September 2020

Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-promoting bacteria. We selected five Parabacteroides distasonis strains isolated from human adult and neonates gut microbiota. We evaluated in vitro their immunomodulation capacities and their ability to reinforce the gut barrier and characterized in vivo their protective effects in an acute murine model of colitis. The in vitro beneficial activities were highly strain dependent: two strains exhibited a potent anti-inflammatory potential and restored the gut barrier while a third strain reinstated the epithelial barrier. While their survival to in vitro gastric conditions was variable, the levels of P. distasonis DNA were higher in the stools of bacteria-treated animals. The strains that were positively scored in vitro displayed a strong ability to rescue mice from colitis. We further showed that two strains primed dendritic cells to induce regulatory T lymphocytes from naïve CD4+ T cells. This study provides better insights on the functionality of commensal bacteria and crucial clues to design live biotherapeutics able to target inflammatory chronic diseases such as IBD.
RBM10, a New Regulator of p53
Authors: Jung Ji Hoon , Lee Hyemin , Zeng Shelya X , Lu Hua
Published: September 2020

The tumor suppressor p53 acts as a transcription factor that regulates the expression of a number of genes responsible for DNA repair, cell cycle arrest, metabolism, cell migration, angiogenesis, ferroptosis, senescence, and apoptosis. It is the most commonly silenced or mutated gene in cancer, as approximately 50% of all types of human cancers harbor TP53 mutations. Activation of p53 is detrimental to normal cells, thus it is tightly regulated via multiple mechanisms. One of the recently identified regulators of p53 is RNA-binding motif protein 10 (RBM10). RBM10 is an RNA-binding protein frequently deleted or mutated in cancer cells. Its loss of function results in various deformities, such as cleft palate and malformation of the heart, and diseases such as lung adenocarcinoma. In addition, RBM10 mutations are frequently observed in lung adenocarcinomas, colorectal carcinomas, and pancreatic ductal adenocarcinomas. RBM10 plays a regulatory role in alternative splicing. Several recent studies not only linked this splicing regulation of RBM10 to cancer development, but also bridged RBM10′s anticancer function to the p53 pathway. This review will focus on the current progress in our understanding of RBM10 regulation of p53, and its role in p53-dependent cancer prevention.
Raloxifene and n-Acetylcysteine Ameliorate TGF-Signalling in Fibroblasts from Patients with Recessive Dominant Epidermolysis Bullosa
Authors: Aguado Tania , García Marta , García Adela , Ferrer-Mayorga Gemma , Martínez-Santamaría Lucía , del Río Marcela , Botella Luisa-María , Sánchez-Puelles José-María
Published: September 2020

Recessive dystrophic epidermolysis bullosa (RDEB) is a severe skin disease caused by mutation of the COL7A1 gene. RDEB is associated with high levels of TGF-β1, which is likely to be involved in the fibrosis that develops in this disease. Endoglin (CD105) is a type III coreceptor for TGF-β1 and its overexpression in fibroblasts deregulates physiological Smad/Alk1/Alk5 signalling, repressing the synthesis of TGF-β1 and extracellular matrix (ECM) proteins. Raloxifene is a specific estrogen receptor modulator designated as an orphan drug for hereditary hemorrhagic telangiectasia, a rare vascular disease. Raloxifene stimulates endoglin synthesis, which could attenuate fibrosis. By contrast, the antioxidant N-acetylcysteine may have therapeutic value to rectify inflammation, fibrosis and endothelial dysfunction. Thus, we present here a repurposing strategy based on the molecular and functional screening of fibroblasts from RDEB patients with these drugs, leading us to propose the repositioning of these two well-known drugs currently in clinical use, raloxifene and N-acetylcysteine, to counteract fibrosis and inflammation in RDEB. Both compounds modulate the profibrotic events that may ultimately be responsible for the clinical manifestations in RDEB, suggesting that these findings may also be relevant for other diseases in which fibrosis is an important pathophysiological event.
Pulmonary Hypertension with Prolonged Patency of the Ductus Arteriosus in Preterm Infants
Authors: Philip Ranjit , Lamba Vineet , Talati Ajay , Sathanandam Shyam
Published: September 2020

There continues to be a reluctance to close the patent ductus arteriosus (PDA) in premature infants. The debate on whether the short-term outcomes translate to a difference in long-term benefits remains. This article intends to review the pulmonary vasculature changes that can occur with a chronic hemodynamically significant PDA in a preterm infant. It also explains the rationale and decision-making involved in a diagnostic cardiac catheterization and transcatheter PDA closure in these preterm infants.
Effect of CeO2-ZnO Nanocomposite for Photocatalytic and Antibacterial Activities
Authors: Syed Asad , Yadav Lakshmi Sagar Reddy , Bahkali Ali H. , Elgorban Abdallah M. , Abdul Hakeem Deshmukh , Ganganagappa Nagaraju
Published: September 2020

The impact of a CeO2-ZnO nanocomposite on the photocatalytic and antibacterial properties compared to bare ZnO was investigated. A CeO2-ZnO nanocomposite was synthesized using Acacia nilotica fruit extract as a novel fuel by a simple solution combustion method. The obtained CeO2-ZnO nanocomposite was confirmed structurally by XRD, FTIR, Raman and UV-DRS and morphologically by SEM/TEM analysis. The XRD pattern indicates the presence of both hexagonal Wurtzite-structured ZnO (major) and cubic-phase CeO2 (minor). FTIR shows the presence of a Ce-O-Ce vibration at 468 cm−1 and Zn-O vibration at 445 cm−1. The existence of a band at 460 cm−1 confirmed the F2g Raman-active mode of the fluorite cubic crystalline structure for CeO2. Diffused reflectance spectroscopy was used to estimate the bandgap (Eg) from Kubelka–Munk (K–M) theory which was found to be 3.4 eV. TEM analysis shows almost spherical-shaped particles, at a size of about 10–15 nm. The CeO2-ZnO nanocomposite shows a good BET specific surface area of 30 m2g−1. The surface defects and porosity of the CeO2-ZnO nanocomposite caused methylene blue (MB) dye to degrade under sunlight (88%) and UV light (92%). The CeO2-ZnO nanocomposite also exhibited considerable antibacterial activity against a pathogenic bacterial strain.
Use of the Correlation between Grain Size and Crystallographic Orientation in Crystal Plasticity Simulations: Application to AISI 420 Stainless Steel
Authors: Galán-López Jesús , Hidalgo Javier
Published: September 2020

Crystal plasticity models attempt to reproduce the complex deformation processes of polycrystalline metals based on a virtual representation of the real microstructure. When choosing this representation, a compromise must be made between level of detail at the local level and statistical significance of the aggregate properties, also taking into account the computational cost of each solution. In this work, the correlation between crystallographic orientation and grain size is considered in the definition of virtual microstructures for the simulation of the mechanical behavior of AISI 420 stainless steel (consisting of a ferrite matrix with large carbide precipitates), in order to improve the accuracy of the solution without increasing model complexity or computation time. Both full-field (DAMASK) and mean-field models (Visco Plastic Self Consistent (VPSC)) are used together in combination with experimental results to study the validity of the assumptions done in each of the models.
The Extraordinary Importance of Coral-Associated Fauna
Authors: Montano Simone
Published: September 2020

Coral reefs are one of the most diverse marine ecosystems on Earth and one of the richest in terms of species interactions. Scleractinian corals are usually the most likely to provide numerous different habitats and to support many symbiotic relationships. However, many other invertebrate groups, such as sponges, bryozoans, and other cnidarians, establish strict symbiotic relationships with other marine organisms. Despite the nature of these relationships—as well as the factors that drive their establishment—being unclear in most cases, a few studies have already shown that some associations may increase the resistance of their hosts to external disturbances. Thus, the potential ability of each member of these diverse symbiotic assemblages to influence the fitness and long-term survival of their hosts bring the coral-associated fauna to the top of the list of coral reef studies. Unfortunately, the widespread degradation of coral reef ecosystems may threaten the existence of the intimate relationships that may go unrecognized complicating our understanding of the intricate networks connecting the fates of reef species. Therefore, this unprecedented loss of biodiversity calls for synergic conservation and monitoring actions aimed at significantly increasing our efforts to search for and describe as much of the diversity of coral-associated organisms as possible, shedding new light on the complex, elusive mechanisms controlling coral reef functioning.
Cerebral White Matter Lesions on Diffusion-Weighted Images and Delayed Neurological Sequelae after Carbon Monoxide Poisoning: A Prospective Observational Study
Authors: Nah Sangun , Choi Sungwoo , Kim Han Bit , Lee Jungbin , Lee Sun-Uk , Lee Young Hwan , Kim Gi Woon , Han Sangsoo
Published: September 2020

Introduction: Carbon monoxide (CO) poisoning can result in delayed neurological sequelae (DNS). Factors predicting DNS are still controversial. This study aims to determine whether acute brain lesions observed using diffusion-weighted magnetic resonance imaging (MRI) following acute CO poisoning are related to the subsequent development of DNS. Methods: This prospective study was conducted on patients with CO poisoning treated at a university hospital in Bucheon, Korea. From August 2016 to July 2019, a total of 283 patients visited the hospital because of CO poisoning. Exclusion criteria included age under 18 years, refusing hyperbaric oxygen therapy, refusing MRI, being discharged against medical advice, being lost to follow-up, having persistent neurological symptoms at discharge, and being transferred from another hospital 24 h after exposure. Results: Of the 154 patients included in the final study, acute brain lesions on MRI (ABLM) were observed in 49 patients (31.8%) and DNS occurred in 30 patients (19.5%). In a logistic regression analysis, lower Glasgow coma scale score and higher exposure time were associated with DNS, and the presence of ABLM in white matter was significantly associated with DNS (OR 6.741; 95% CI, 1.843–24.660; p = 0.004). Conclusion: The presence of ABLM in white matter was significantly related to the occurrence of DNS. Early prediction of the risk of developing DNS through MRI may be helpful in treating patients with CO poisoning.
Association of Bone Mineral Density and Coronary Artery Calcification in Patients with Osteopenia and Osteoporosis
Authors: Chuang Tzyy-Ling , Koo Malcolm , Wang Yuh-Feng
Published: September 2020

The aim of this study was to investigate the association between bone mineral density (BMD) and coronary artery calcification (CAC) in adults with osteopenia or osteoporosis. A retrospective medical review study was conducted in a regional hospital in southern Taiwan. Medical records of patients who underwent both a coronary computed tomography scan and a BMD measurement were identified. Multinomial logistic regression analyses were used to assess the association between BMD and CAC levels in patients with osteopenia or osteoporosis. Of the 246 patients, 119 were female and 42.3% had CAC. For patients with osteopenia, after adjusting for the significant factors of CAC, no significant association was observed between BMD with either moderate CAC (0 < CAC score ≤ 100) or high CAC (CAC score > 100). However, in patients with osteoporosis, after adjusting for the significant factors of CAC, BMD in the lumbar spine was inversely associated with moderate CAC (odds ratio = 0.38, p = 0.035). In conclusion, a lower BMD in the lumbar spine was associated with an increased risk of moderate CAC in patients with osteoporosis. It is crucial to take action to maintain bone health, particularly in those who already have osteoporosis, to reduce the risk of developing CAC and its associated morbidity and mortality.
Analytic Hierarchy Process (AHP)-Based Aggregation Mechanism for Resilience Measurement: NATO Aggregated Resilience Decision Support Model
Authors: Hodicky Jan , Özkan Gökhan , Özdemir Hilmi , Stodola Petr , Drozd Jan , Buck Wayne
Published: September 2020

Resilience is a complex system that represents dynamic behaviours through its complicated structure with various nodes, interrelations, and information flows. Like other international organizations NATO has also been dealing with the measurement of this complex phenomenon in order to have a comprehensive understanding of the civil environment and its impact on military operations. With this ultimate purpose, NATO had developed and executed a prototype model with the system dynamics modelling and simulation paradigm. NATO has created an aggregated resilience model as an upgrade of the prototype one, as discussed within this study. The structure of the model, aggregation mechanism and shock parametrization methodologies used in the development of the model comprise the scope of this study. Analytic Hierarchy Process (AHP), which is a multi-criteria decision-making technique is the methodology that is used for the development of the aggregation mechanism. The main idea of selecting the AHP methodology is its power and usefulness in mitigating bias in the decision-making process, its capability to increase the number of what-if scenarios to be created, and its contribution to the quality of causal explanations with the granularity it provides. The parametrized strategic shock input page, AHP-based weighted resilience and risk parameters input pages, one more country insertion to the model, and the decision support system page enhance the capacity of the prototype model. As part of the model, the decision support system page stands out as the strategic level cockpit where the colour codes give a clear idea at first about the overall situational picture and country-wise resilience and risk status. At the validation workshop, users not only validated the model but also discussed further development opportunities, such as adding more strategic shocks into the model and introduction of new parameters that will be determined by a big data analysis on relevant open source databases. The developed model has the potential to inspire high-level decision-makers dealing with resilience management in other international organizations, such as the United Nations.
An Agent-Based Approach for the Planning of Distribution Grids as a Socio-Technical System
Authors: Siebert Luciano Cavalcante , Aoki Alexandre Rasi , Lambert-Torres Germano , Lambert-de-Andrade Nelson , Paterakis Nikolaos G.
Published: September 2020

Recent developments, such as smart metering, distributed energy resources, microgrids, and energy storage, have led to an exponential increase in system complexity and have emphasized the need to include customer behavior and social and cultural backgrounds in planning activities. This paper analyzes how emergent behavior in electricity consumption can affect the planning of distribution grids with a smart grid vision. For this, an agent-based model that uses insights from the field of behavioral economics to differentiate four consumer categories (high income, low income, middle class, and early adopters) was used. The model was coupled with a real distribution feeder and customer load curve data, and the results showed that heterogeneity of customer’s preferences, values, and behavior led to very distinct load growth patterns. The results emphasize the relevance of modeling customer’s behavioral aspects in planning increasingly complex power systems.
The Linkage between Economic Growth, Renewable Energy, Tourism, CO2 Emissions, and International Trade: The Evidence for the European Union
Authors: Leitão Nuno Carlos , Lorente Daniel Balsalobre
Published: September 2020

This paper evaluates the link between economic growth, renewable energy, tourism arrivals, trade openness, and carbon dioxide emissions in the European Union (EU-28). As an econometric strategy, the research uses panel data. In the first step, we apply the unit root test, and the results demonstrated that the variables used in this study are integrated I (1) in the first difference. In the second step, we apply the Pedroni cointegration test, and Kao Residual cointegration test, and we observe that the variables are cointegrated in the long run. The panel fully modified least squares (FMOLS), panel dynamic least squares (DOLS), and generalized moments system (GMM-System) estimator are considered in this research. The econometric results proved that trade openness and renewable energy decreased climate change and environmental degradation. The empirical study also found a positive effect of economic growth on carbon dioxide emissions. Moreover, tourism arrivals are negatively correlated with carbon dioxide emissions, showing sustainability practices of the tourism sector on the environment. Furthermore, carbon dioxide emissions in the long run present a positive impact, indicating that climate change increases. In this study, we also consider the recent methodology of Dumitrescu–Hurlin to observe the causality and the relationship between renewable energy, trade openness, economic growth, tourism arrivals, and carbon dioxide emissions.
Stand Structural Diversity and Species with Leaf Nitrogen Conservation Drive Aboveground Carbon Storage in Tropical Old-Growth Forests
Authors: Wang Genzhu , Liu Yuguo , Wu Xiuqin , Pang Danbo , Yang Xiao , Hussain Azfar , Zhou Jinxing
Published: September 2020

Tropical old-growth forests are essential for global carbon regulation. Although there is increasing evidence that species and functional diversity, stand structural diversity, functional compositions, and elevation play roles in ecosystem functioning, the relative strengths of these drivers and the underlying mechanisms (mass-ratio hypothesis or niche complementarity hypothesis) are not clear. Aboveground carbon storage, species diversity, stand structural diversity, community-weighted mean (CWM), and functional diversity (FDvar) of 12 leaf traits were analyzed using data from 56 old-growth forest communities in the Dawei Mountain area of Southwest China. Multiple regression models were used to test the relative importance of the predictor variables and the structural equation model was used to explore the direct and indirect influences on aboveground carbon storage. High structural diversity moderately enhanced aboveground carbon storage. CWM leaf nitrogen concentration in young leaves weakly affected aboveground carbon storage. Our final multiple regression model showed that aboveground carbon storage is mostly affected by diameter at breast height (DBH) diversity, followed by FDvar of dry matter concentration in mature leaves and CWM nitrogen concentration in young leaves. The structural equation model indicated that elevation negatively affects aboveground carbon storage via diameter at breast height (DBH) diversity. Our results suggest that niche complementarity effects moderately drive aboveground carbon storage in tropical old-growth forests, but do not fully support the importance of the mass-ratio hypothesis.
The Meaning of Emoji to Describe Food Experiences in Pre-Adolescents
Authors: Sick Julia , Monteleone Erminio , Pierguidi Lapo , Ares Gastón , Spinelli Sara
Published: September 2020

Ongoing research has shown that emoji can be used by children to discriminate food products, but it is unclear if they express emotions and how they are linked to emotional words. Little is known about how children interpret emoji in terms of their emotional meaning in the context of food. This study aimed at investigating the emotional meaning of emoji used to describe food experiences in 9–13-year-old pre-adolescents and to measure related age and gender differences. The meaning of 46 emoji used to describe food experience was explored by: mapping emoji according to similarities and differences in their emotional meaning using the projective mapping technique, and linking emoji with emotion words using a check-all-that-apply (CATA) format. The two tasks gave consistent results and showed that emoji were discriminated along the valence (positive vs. negative) and power (dominant vs. submissive) dimension, and to a lower extent along the arousal dimension (high vs. low activation). In general, negative emoji had more distinct meanings than positive emoji in both studies, but differences in nuances of meaning were found also among positive emoji. Girls and older pre-adolescents (12–13 years old (y.o.)) discriminated positive emoji slightly better than boys and younger pre-adolescents (9–11 y.o.). This suggests that girls and older pre-adolescents may be higher in emotional granularity (the ability to experience and discriminate emotions), particularly of positive emotions. The results of the present work can be used for the development of an emoji-based tool to measure emotions elicited by foods in pre-adolescents.
Estimating Case-Based Learning
Authors: Guilfoos Todd , Pape Andreas Duus
Published: September 2020

We propose a framework in order to econometrically estimate case-based learning and apply it to empirical data from twelve 2 × 2 mixed strategy equilibria experiments. Case-based learning allows agents to explicitly incorporate information available to the experimental subjects in a simple, compact, and arguably natural way. We compare the estimates of case-based learning to other learning models (reinforcement learning and self-tuned experience weighted attraction learning) while using in-sample and out-of-sample measures. We find evidence that case-based learning explains these data better than the other models based on both in-sample and out-of-sample measures. Additionally, the case-based specification estimates how factors determine the salience of past experiences for the agents. We find that, in constant sum games, opposing players’ behavior is more important than recency and, in non-constant sum games, the reverse is true.
Sediment Bed-Load Transport: A Standardized Notation
Authors: Zanke Ulrich , Roland Aron
Published: September 2020

Morphodynamic processes on Earth are a result of sediment displacements by the flow of water or the action of wind. An essential part of sediment transport takes place with permanent or intermittent contact with the bed. In the past, numerous approaches for bed-load transport rates have been developed, based on various fundamental ideas. For the user, the question arises which transport function to choose and why just that one. Different transport approaches can be compared based on measured transport rates. However, this method has the disadvantage that any measured data contains inaccuracies that correlate in different ways with the transport functions under comparison. Unequal conditions also exist if the factors of transport functions under test are fitted to parts of the test data set during the development of the function, but others are not. Therefore, a structural formula comparison is made by transferring altogether 13 transport functions into a standardized notation. Although these formulas were developed from different perspectives and with different approaches, it is shown that these approaches lead to essentially the same basic formula for the main variables. These are shear stress and critical shear stress. However, despite the basic structure of these 13 formulas being the same, their coefficients vary significantly. The reason for that variation and the possible effect on the bandwidth of results is identified and discussed. A further result is the finding that not only shear stress affects bed-load transport rates as is expressed by many transport formulas. Transport rates are also significantly affected by the internal friction of the moving sediment as well as by the friction fluid-bed. In the case of not fully rough flow conditions, also viscous effects and thus the Reynolds number becomes of importance.
Stock Market Reactions to COVID-19 Pandemic Outbreak: Quantitative Evidence from ARDL Bounds Tests and Granger Causality Analysis
Authors: Gherghina Ștefan Cristian , Armeanu Daniel Ștefan , Joldeș Camelia Cătălina
Published: September 2020

This paper examines the linkages in financial markets during coronavirus disease 2019 (COVID-19) pandemic outbreak. For this purpose, daily stock market returns were used over the period of December 31, 2019–April 20, 2020 for the following economies: USA, Spain, Italy, France, Germany, UK, China, and Romania. The study applied the autoregressive distributed lag (ARDL) model to explore whether the Romanian stock market is impacted by the crisis generated by novel coronavirus. Granger causality was employed to investigate the causalities among COVID-19 and stock market returns, as well as between pandemic measures and several commodities. The outcomes of the ARDL approach failed to find evidence towards the impact of Chinese COVID-19 records on the Romanian financial market, neither in the short-term, nor in the long-term. On the other hand, our quantitative approach reveals a negative effect of the new deaths’ cases from Italy on the 10-year Romanian bond yield both in the short-run and long-run. The econometric research provide evidence that Romanian 10-year government bond is more sensitive to the news related to COVID-19 than the index of the Bucharest Stock Exchange. Granger causality analysis reveals causal associations between selected stock market returns and Philadelphia Gold/Silver Index.
A Novel Anti-Environmental Forest Experience Scale to Predict Preferred Pleasantness Associated with Forest Environments
Authors: Bielinis Ernest , Xu Jianzhong , Omelan Aneta Anna
Published: September 2020

In this study, a method for predicting the preferred pleasantness induced by different forest environments, represented by virtual photographs, was proposed and evaluated using a novel Anti-Environmental Forest Experience Scale psychometric test. The evaluation questionnaire contained twenty-one items divided into four different subscales. The factor structure was assessed in two separate samples collected online (sample 1: n = 254, sample 2: n = 280). The internal validity of the four subscales was confirmed using exploratory factor analysis. Discriminant validity was tested and confirmed using the Amoebic Self Scale (spatial–symbolic domain). Concurrent validity was confirmed using the Connectedness to Nature Scale. Predictive validity was based on an assessment of pleasantness induced by nine different photographs (control—urban landscapes, forest landscapes, dense forest landscapes), with subscales differently correlated with the level of pleasantness assessed for each photograph. This evaluation instrument is appropriate for predicting preferred pleasantness induced by different forest environments.
Reliability of the Resonance Frequency Analysis Values in New Prototype Transepithelial Abutments: A Prospective Clinical Study
Authors: Guerrero-González María , Monticelli Francesca , Saura García-Martín David , Herrero-Climent Mariano , Ríos-Carrasco Blanca , Ríos-Santos José-Vicente , Fernández-Palacín Ana
Published: September 2020

Resonance frequency analysis (RFA) requires abutment disconnection to monitor implant stability. To overcome this limitation, an experimental transepithelial abutment was designed to allow a SmartPeg to be screwed onto it, in order to determine the prototype abutments repeatability and reproducibility using Osstell ISQ and to assess whether implant length and diameter have an influence on the reliability of these measurements. RFA was conducted with a SmartPeg screwed directly into the implant and onto experimental abutments of different heights of 2, 3.5 and 5 mm. A total of 32 patients (116 implants) were tested. RFA measurements were taken twice for each group from mesial, distal, buccal and palatal/lingual surfaces. Mean values and SD were calculated and Intraclass Correlation Coefficients (ICC) (p < 0.05, IC 95%). The implant stability quotient (ISQ) mean values were 72.581 measured directly to implant and 72.899 (2 mm), 72.391 (3.5 mm) and 71.458 (5 mm) measured from the prototypes. ICC between measurements made directly to implant and through 2-, 3.5- and 5-mm abutments were 0.908, 0.919 and 0.939, respectively. RFA values registered through the experimental transepithelial abutments achieved a high reliability. Neither the implant length nor the diameter had any influence on the measurements’ reliability.
The Psychological Process of Residents’ Acceptance of Local Shale Gas Exploitation in China
Authors: Yao Liuyang , Sui Dangchen , Liu Xiaotong , Fan Hui
Published: September 2020

Local communities and their opinion on shale gas exploitation (SGE) play an essential role in the implementation of energy policies, while little is known about the reasoning process underpinning the acceptance of SGE. The present study develops a conceptual framework to examine the psychological process of residents’ acceptance of local SGE, in which the impacts of trust, knowledge, and fairness are mediated by risk and benefit perceptions. Structural equation modeling has been applied to analyze the hypothesized relationships based on a dataset of 825 households in China’s largest shale gas field. Our results indicate that residents’ perceived fairness and trust positively affect their benefit perceptions and negatively affect their risk perceptions, which results in positive influences on acceptance, and knowledge of SGE’s environmental impacts positively affects perceived risks, which results in a negative influence on acceptance. Moreover, residents’ acceptance is primarily determined by their benefit perception, followed by perceived fairness, and knowledge is the least important determinant. Thus, our study contributes to the literature by exploring the structural relationships between various psychological predictors and the acceptance toward SGE, and the results from our empirical survey provide insight into designing appropriate strategies in the process of generating and communicating shale policies.
Health and Well-Being in Protected Natural Areas—Visitors’ Satisfaction in Three Different Protected Natural Area Categories in Catalonia, Spain
Authors: Farías-Torbidoni Estela Inés , Mas-Alòs Sebastià , Gil-Moreno-de-Mora Gonzalo , Lavega-Burgués Pere , Castañer Marta , Lorente-Catalán Eloisa , Seguí-Urbaneja Jordi , Lacasa-Claver Enric
Published: September 2020

Protected natural areas (PNAs) can be a source of health and well-being, but little research has been carried out regarding outcomes in terms of satisfaction (the difference between motivations and benefits). Inspired by previous research that examines the motivations and benefits perceived by visitors to various PNAs in Canada, and based on importance–performance analysis (IPA) and service quality gap (GAP) analysis theory, the aim of this study was to identify the outcomes generated by protected areas in terms of satisfaction, especially with regard to the PNAs’ individual protection categories. The study was based on survey data from visitors (n = 360) to three PNAs in Catalonia: one national park, one natural park and one periurban park. The results indicate that anticipated environmental, psychological, physical and social benefits were of major personal value in choosing to visit a PNA. The results indicate that, generally, visitors were satisfied with regard to the benefits anticipated. Differences between parks in this respect could be explained in part by sociodemographic factors and visitors’ behavior. The results are discussed in terms of their applicability and how they relate to the role of PNAs in the promotion of visitors’ health and well-being.
Analysis of the Influence of Psychological Contract on Employee Safety Behaviors against COVID-19
Authors: Du Yuexin , Liu Hui
Published: September 2020

This study explored the influencing factors of safety behavior from the perspective of employees, studied the mechanism of the psychological contract on employees’ safety behavior in the context of the Chinese epidemic situation, tested the mediating role of job burnout and perceived insider status in the process of work resumption, and provided preventive suggestions for combating the global spread of COVID-19. A questionnaire survey was utilized to collect data and, combined with the necessary protective measures taken for employees in China, was used to modify the mature safety behavior scale. Finally, through the analysis of 402 employees’ questionnaires, the hypotheses were verified; that is, in the process of Chinese enterprises returning to work to cope with COVID-19, the psychological contract has a positive role in promoting employees’ safety behavior, while job burnout plays a weakened mediating role, and perceived insider status plays a strengthening mediating role. The psychological contract negatively affects job burnout but positively affects perceived insider status. Job burnout negatively affects employees’ safety behavior, but perceived insider status positively affects employees’ safety behavior. The results show that employees’ conscious participation in safety behavior plays an irreplaceable role in the prevention of COVID-19 and safety of work resumption.
The City of Tomorrow from… the Data of Today
Authors: Agugiaro Giorgio , González Francisco , Cavallo Roberto
Published: September 2020

In urban planning, a common unit of measure for housing density is the number of households per hectare. However, the actual size of the physical space occupied by a household, i.e., a dwelling, is seldom considered, neither in 2D nor in 3D. This article proposes a methodology to estimate the average size of a dwelling in existing urban areas from available open data, and to use it as one of the design parameters for new urban-development projects. The proposed unit of measure, called “living space”, includes outdoor and indoor spaces. The idea is to quantitatively analyze the city of today to help design the city of tomorrow. First, the “typical”-dwelling size and a series of Key Performance Indicators are computed for all neighborhoods from a semantic 3D city model and other spatial and non-spatial datasets. A limited number of neighborhoods is selected based on their similarities with the envisioned development plan. The size of the living space of the selected neighborhoods is successively used as a design parameter to support the computer-assisted generation of several design proposals. Each proposal can be exported, shared, and visualized online. As a test case, a to-be-planned neighborhood in Amsterdam, called “Sloterdijk One”, has been chosen.
Combination of High Dose Hypofractionated Radiotherapy with Anti-PD1 Single Dose Immunotherapy Leads to a Th1 Immune Activation Resulting in a Complete Clinical Response in a Melanoma Patient
Authors: Milhem Clara , Moralès Olivier , Ingelaere Céline , Pasquier David , Mordon Serge , Mortier Laurent , Mirabel Xavier , Delhem Nadira
Published: September 2020

The development of immunotherapy has recently modified the anti-tumor therapeutic arsenal; particularly, immune checkpoint inhibitors have led to a significant increase in overall survival. The current challenge is now to select good responder patients by identifying early biomarkers to propose therapeutic combinations that potentiate the efficacy of the therapy. Here we report the case of a 60-year-old man with superficial melanoma treated with high-dose hypo fractionated radiotherapy (H-SRT) combined with a single dose of anti-PD1 immunotherapy (Nivolumab) for a metastatic lymph node recurrence due to cancer progression. In this study, we present the results obtained regarding the activation of the Th1 immune response after H-SRT treatment followed by anti PD-1 therapeutic protocol. These results were correlated with clinical data to identify potential immunological biomarkers of treatment efficacy. This exceptional case report shows that a combination of H-SRT with a single dose of anti-PD1 immunotherapy may allow a better activation of the immune response in favor of a complete clinical response.
Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction
Authors: Rey Federica , Pandini Cecilia , Barzaghini Bianca , Messa Letizia , Giallongo Toniella , Pansarasa Orietta , Gagliardi Stella , Brilli Matteo , Zuccotti Gian Vincenzo , Cereda Cristina , Raimondi Manuela Teresa , Carelli Stephana
Published: September 2020

3D cell cultures are becoming more and more important in the field of regenerative medicine due to their ability to mimic the cellular physiological microenvironment. Among the different types of 3D scaffolds, we focus on the Nichoid, a miniaturized scaffold with a structure inspired by the natural staminal niche. The Nichoid can activate cellular responses simply by subjecting the cells to mechanical stimuli. This kind of influence results in different cellular morphology and organization, but the molecular bases of these changes remain largely unknown. Through RNA-Seq approach on murine neural precursors stem cells expanded inside the Nichoid, we investigated the deregulated genes and pathways showing that the Nichoid causes alteration in genes strongly connected to mechanobiological functions. Moreover, we fully dissected this mechanism highlighting how the changes start at a membrane level, with subsequent alterations in the cytoskeleton, signaling pathways, and metabolism, all leading to a final alteration in gene expression. The results shown here demonstrate that the Nichoid influences the biological and genetic response of stem cells thorough specific alterations of cellular signaling. The characterization of these pathways elucidates the role of mechanical manipulation on stem cells, with possible implications in regenerative medicine applications.
The Molecular Priming of Defense Responses is Differently Regulated in Grapevine Genotypes Following Elicitor Application against Powdery Mildew
Authors: Pagliarani Chiara , Moine Amedeo , Chitarra Walter , Meloni Giovanna Roberta , Abbà Simona , Nerva Luca , Pugliese Massimo , Gullino Maria Lodovica , Gambino Giorgio
Published: September 2020

Molecular changes associated with response to powdery mildew (PM) caused by Erysiphe necator have been largely explored in Vitis vinifera cultivars, but little is known on transcriptional and metabolic modifications following application of resistance elicitors against this disease. In this study, the whole transcriptome sequencing, and hormone and metabolite analyses were combined to dissect long-term defense mechanisms induced by molecular reprogramming events in PM-infected ‘Moscato’ and ‘Nebbiolo’ leaves treated with three resistance inducers: acibenzolar-S-methyl, potassium phosphonate, and laminarin. Although all compounds were effective in counteracting the disease, acibenzolar-S-methyl caused the most intense transcriptional modifications in both cultivars. These involved a strong down-regulation of photosynthesis and energy metabolism and changes in carbohydrate accumulation and partitioning that most likely shifted the plant growth-defense trade-off towards the establishment of disease resistance processes. It was also shown that genotype-associated metabolic signals significantly affected the cultivar defense machinery. Indeed, ‘Nebbiolo’ and ‘Moscato’ built up different defense strategies, often enhanced by the application of a specific elicitor, which resulted in either reinforcement of early defense mechanisms (e.g., epicuticular wax deposition and overexpression of pathogenesis-related genes in ‘Nebbiolo’), or accumulation of endogenous hormones and antimicrobial compounds (e.g., high content of abscisic acid, jasmonic acid, and viniferin in ‘Moscato’).
The Aryl Hydrocarbon Receptor: A Mediator and Potential Therapeutic Target for Ocular and Non-Ocular Neurodegenerative Diseases
Authors: Choudhary Mayur , Malek Goldis
Published: September 2020

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor, which senses environmental, dietary or metabolic signals to mount a transcriptional response, vital in health and disease. As environmental stimuli and metabolic products have been shown to impact the central nervous system (CNS), a burgeoning area of research has been on the role of the AHR in ocular and non-ocular neurodegenerative diseases. Herein, we summarize our current knowledge, of AHR-controlled cellular processes and their impact on regulating pathobiology of select ocular and neurodegenerative diseases. We catalogue animal models generated to study the role of the AHR in tissue homeostasis and disease pathogenesis. Finally, we discuss the potential of targeting the AHR pathway as a therapeutic strategy, in the context of the maladies of the eye and brain.
Structure, Folding and Stability of Nucleoside Diphosphate Kinases
Authors: Georgescauld Florian , Song Yuyu , Dautant Alain
Published: September 2020

Nucleoside diphosphate kinases (NDPK) are oligomeric proteins involved in the synthesis of nucleoside triphosphates. Their tridimensional structure has been solved by X-ray crystallography and shows that individual subunits present a conserved ferredoxin fold of about 140 residues in prokaryotes, archaea, eukaryotes and viruses. Monomers are functionally independent from each other inside NDPK complexes and the nucleoside kinase catalytic mechanism involves transient phosphorylation of the conserved catalytic histidine. To be active, monomers must assemble into conserved head to tail dimers, which further assemble into hexamers or tetramers. The interfaces between these oligomeric states are very different but, surprisingly, the assembly structure barely affects the catalytic efficiency of the enzyme. While it has been shown that assembly into hexamers induces full formation of the catalytic site and stabilizes the complex, it is unclear why assembly into tetramers is required for function. Several additional activities have been revealed for NDPK, especially in metastasis spreading, cytoskeleton dynamics, DNA binding and membrane remodeling. However, we still lack the high resolution structural data of NDPK in complex with different partners, which is necessary for deciphering the mechanism of these diverse functions. In this review we discuss advances in the structure, folding and stability of NDPKs.
TIE1 as a Candidate Gene for Lymphatic Malformations with or without Lymphedema
Authors: Michelini Sandro , Ricci Maurizio , Veselenyiova Dominika , Kenanoglu Sercan , Kurti Danjela , Baglivo Mirko , Fiorentino Alessandro , Basha Syed Hussain , Priya Sasi , Serrani Roberta , Krajcovic Juraj , Dundar Munis , Dautaj Astrit , Bertelli Matteo
Published: September 2020

TIE1 is a cell surface protein expressed in endothelial cells. Involved in angiogenesis and lymphangiogenesis, including morphogenesis of lymphatic valves, TIE1 is important for lymphatic system functional integrity. The main purpose of this study was to identify different variants in the TIE1 gene that could be associated with lymphatic malformations or dysfunction and predisposition for lymphedema. In a cohort of 235 Italian lymphedema patients, who tested negative for variants in known lymphedema genes, we performed a further test for new candidate genes, including TIE1. Three probands carried different variants in TIE1. Two of these segregated with lymphedema or lymphatic dysfunction in familial cases. Variants in TIE1 could contribute to the onset of lymphedema. On the basis of our findings, we propose TIE1 as a candidate gene for comprehensive genetic testing of lymphedema.
Gut Microbiota-Mediated Inflammation and Gut Permeability in Patients with Obesity and Colorectal Cancer
Authors: Sánchez-Alcoholado Lidia , Ordóñez Rafael , Otero Ana , Plaza-Andrade Isaac , Laborda-Illanes Aurora , Medina José Antonio , Ramos-Molina Bruno , Gómez-Millán Jaime , Queipo-Ortuño María Isabel
Published: September 2020

Obesity is considered an important factor that increases the risk of colorectal cancer (CRC). So far, the association of gut microbiota with both obesity and cancer has been described independently. Nevertheless, a specific obesity-related microbial profile linked to CRC development has not been identified. The aim of this study was to determine the gut microbiota composition in fecal samples from CRC patients with (OB-CRC) and without obesity (L-CRC) compared to the microbiota profile present in non-obese healthy controls (L-HC), in order to unravel the possible relationship between gut microbiota and microbial-derived metabolite trimethylamine N-oxide (TMAO), the inflammatory status, and the intestinal permeability in the context of obesity-associated CRC. The presence of obesity does not induce significant changes in the diversity and richness of intestinal bacteria of CRC patients. Nevertheless, OB-CRC patients display a specific gut microbiota profile characterized by a reduction in butyrate-producing bacteria and an overabundance of opportunistic pathogens, which in turn could be responsible, at least in part, for the higher levels of proinflammatory cytokine IL-1β, the deleterious bacterial metabolite TMAO, and gut permeability found in these patients. These results suggest a possible role of obesity-related gut microbiota in the development of CRC, which could give new clues for the design of new diagnostic tools for CRC prevention.
Humic Substances Mitigate the Impact of Tritium on Luminous Marine Bacteria. Involvement of Reactive Oxygen Species
Authors: Rozhko Tatiana V. , Kolesnik Olga V. , Badun Gennadii A. , Stom Devard I. , Kudryasheva Nadezhda S.
Published: September 2020

The paper studies the combined effects of beta-emitting radionuclide tritium and Humic Substances (HS) on the marine unicellular microorganism—luminous bacteria—under conditions of low-dose radiation exposures (<0.04 Gy). Tritium was used as a component of tritiated water. Bacterial luminescence intensity was considered as a tested physiological parameter. The bioluminescence response of the marine bacteria to tritium corresponded to the “hormesis” model: it included stages of bioluminescence inhibition and activation, as well as the absence of the effect. HS were shown to decrease the inhibition and activation effects of tritium, similar to those of americium-241, alpha-emitting radionuclide, studied earlier. Correlations between the bioluminescence intensity and the content of Reactive Oxygen Species (ROS) were found in the radioactive bacterial suspensions. The results demonstrate an important role of HS in natural processes in the regions of low radioactive contamination: HS can mitigate radiotoxic effects and adaptive response of microorganisms to low-dose radioactive exposures. The involvement of ROS in these processes was demonstrated.