bluuebunny/crossref_metadata_2025 huggingface dataset:
Selection of Newly Identified Growth-Promoting Archaea Haloferax Species With a Potential Action on Cobalt Resistance in Maize Plants
Authors: Selim Samy , Akhtar Nosheen , Hagagy Nashwa , Alanazi Awadh , Warrad Mona , El Azab Eman , Elamir Mohammed Yagoub Mohammed , Al-Sanea Mohammad M. , Jaouni Soad K. Al , Abdel-Mawgoud Mohamed , Shah Anis Ali , Abdelgawad Hamada
Published: May 2022

Soil contamination with cobalt (Co) negatively impacts plant growth and production. To combat Co toxicity, plant growth-promoting microorganisms for improving plant growth are effectively applied. To this end, unclassified haloarchaeal species strain NRS_31 (OL912833), belonging to Haloferax genus, was isolated, identified for the first time, and applied to mitigate the Co phytotoxic effects on maize plants. This study found that high Co levels in soil lead to Co accumulation in maize leaves. Co accumulation in the leaves inhibited maize growth and photosynthetic efficiency, inducing oxidative damage in the tissue. Interestingly, pre-inoculation with haloarchaeal species significantly reduced Co uptake and mitigated the Co toxicity. Induced photosynthesis improved sugar metabolism, allocating more carbon to defend against Co stress. Concomitantly, the biosynthetic key enzymes involved in sucrose (sucrose-P-synthase and invertases) and proline (pyrroline-5- carboxylate synthetase (P5CS), pyrroline-5-carboxylate reductase (P5CR)) biosynthesis significantly increased to maintain plant osmotic potential. In addition to their osmoregulation potential, soluble sugars and proline can contribute to maintaining ROS hemostasis. Maize leaves managed their oxidative homeostasis by increasing the production of antioxidant metabolites (such as phenolics and tocopherols) and increasing the activity of ROS-scavenging enzymes (such as POX, CAT, SOD, and enzymes involved in the AsA/GSH cycle). Inside the plant tissue, to overcome heavy Co toxicity, maize plants increased the synthesis of heavy metal-binding ligands (metallothionein, phytochelatins) and the metal detoxifying enzymes (glutathione S transferase). Overall, the improved ROS homeostasis, osmoregulation, and Co detoxification systems were the basis underlying Co oxidative stress, mitigating haloarchaeal treatment's impact.
Comparative Proteomic Analysis Reveals the Ascorbate Peroxidase-Mediated Plant Resistance to Verticillium dahliae in Gossypium barbadense
Authors: Lu Tianxin , Zhu Liping , Liang Yuxuan , Wang Fei , Cao Aiping , Xie Shuangquan , Chen Xifeng , Shen Haitao , Wang Beini , Hu Man , Li Rong , Jin Xiang , Li Hongbin
Published: May 2022

In previous research on the resistance of cotton to Verticillium wilt (VW), Gossypium hirsutum and G. barbadense were usually used as the susceptible and resistant cotton species, despite their different genetic backgrounds. Herein, we present data independent acquisition (DIA)-based comparative proteomic analysis of two G. barbadense cultivars differing in VW tolerance, susceptible XH7 and resistant XH21. A total of 4,118 proteins were identified, and 885 of them were differentially abundant proteins (DAPs). Eight co-expressed modules were identified through weighted gene co-expression network analysis. GO enrichment analysis of the module that significantly correlated with V. dahliae infection time revealed that oxidoreductase and peroxidase were the most significantly enriched GO terms. The last-step rate-limiting enzyme for ascorbate acid (AsA) biosynthesis was further uncovered in the significantly enriched GO terms of the 184 XH21-specific DAPs. Additionally, the expression of ascorbate peroxidase (APX) members showed quick accumulation after inoculation. Compared to XH7, XH21 contained consistently higher AsA contents and rapidly increased levels of APX expression, suggesting their potential importance for the resistance to V. dahliae. Silencing GbAPX1/12 in both XH7 and XH 21 resulted in a dramatic reduction in VW resistance. Our data indicate that APX-mediated oxidoreductive metabolism is important for VW resistance in cotton.
Three Intervention Programs in Secondary Education on Attitudes Toward Persons With a Disability
Authors: Álvarez-Delgado Julián , León-del-Barco Benito , Polo-del-Río María-Isabel , Mendo-Lázaro Santiago , Lopez-Ramos Victor M.
Published: May 2022

Persons with a disability make up a social group which is in an especially vulnerable situation. They have to face obstacles and difficulties in their participation as part of the community with equal opportunities, in which attitude of others is a determining factor. This study makes a comparative analysis of three intervention programs (1 “Simulation and Modeling,” 2 “Information and Awareness Raising,” and 3 “Adapted Sport”) on attitudes toward persons with a disability of adolescents in secondary schools. Each program is based on a concrete technique, but they all have the common thread of the direct, structured contact technique with persons with a disability. The effectiveness of the three programs in changing attitudes is analyzed, and their impact on the different factors of the attitude construct (1 “acceptance/rejection,” 2 “competence/limitation,” and 3 “equality of opportunities”) is also studied. The results show the effectiveness of the three programs. The students show more positive attitudes toward persons with a disability in all the groups, especially program 1. Analyzing the general influence of the three programs on the factors of the attitude construct, it can be seen that in factors 2 and 3, the attitudes have significantly improved in all three programs. Finally, the results show that each program has been more effective on a concrete attitude factor.
Managing Organizational Inertia: Indonesian Family Business Perspective
Authors: Teofilus Teofilus , Ardyan Elia , Sutrisno Timotius F. C. W. , Sabar Sabar , Sutanto Verrell
Published: May 2022

The ability to transform on a regular basis is critical in the effort to adapt to external challenges; however, changes to an organization’s fundamental characteristics may increase the likelihood of failure. Because of this, organizational restructuring efforts appear to engender cynicism, which appears to be one of the most significant obstacles facing contemporary businesses, particularly in this area. Organizational inertia is the term used to describe this aversion to change, as well as the desire to maintain the current status quo. A new organizational culture capable of combating the incidence of organizational stagnation is required by massive social, economic, and technological difficulties, and firms that employ the concept of empowering leadership will be able to meet these challenges. For the purposes of this study, a framework for discussing the phenomena of organizational cynicism was developed and implemented.
Trapped by the Lack of Control Over Savings: Evidence From Pakistan
Authors: van Dongen Elisa , Ahmad Syedah , Lensink Robert , Mueller Annika
Published: May 2022

Gender discrimination and associated social norms are important contributing factors to the high frequency of women trapped in poverty – particularly in developing countries. Financial inclusion, especially access to formal saving services, has recently received much attention from the development community for its potential to lift women out of poverty and reduce inequality. To date, however, the impacts of social norms on women’s ability to use and benefit from such formal saving services are not widely understood. The purpose of this paper is to advance the understanding of this relationship, by investigating, in a setting where social norms put women at a disadvantage, the association between their decision-making power with respect to a newly opened formal savings account at a bank and the amount of savings kept in that account. We use data on 1,798 married women in Pakistan, from an intervention to encourage savings account uptake among them. Focusing on the usage, 8 months after the intervention, of 512 newly opened bank accounts, we find that women with at least joint control over the bank account save statistically significantly more in this account than women without any control. On average, this difference amounts to 2,339 PKR (22.40 USD), which is substantial considering that the majority of women in our sample are from lower-middle income class households and are rarely the household’s main income earners. This finding has important implications for future research, as well as for policy makers and practitioners providing financial services to women in gender unequal contexts.
Speaker Accent Modulates the Effects of Orthographic and Phonological Similarity on Auditory Processing by Learners of English
Authors: Frances Candice , Navarra-Barindelli Eugenia , Martin Clara D.
Published: May 2022

The cognate effect refers to translation equivalents with similar form between languages—i.e., cognates, such as “band” (English) and “banda” (Spanish)—being processed faster than words with dissimilar forms—such as, “cloud” and “nube.” Substantive literature supports this claim, but is mostly based on orthographic similarity and tested in the visual modality. In a previous study, we found an inhibitory orthographic similarity effect in the auditory modality—i.e., greater orthographic similarity led to slower response times and reduced accuracy. The aim of the present study is to explain this effect. In doing so, we explore the role of the speaker's accent in auditory word recognition and whether native accents lead to a mismatch between the participants' phonological representation and the stimulus. Participants carried out a lexical decision task and a typing task in which they spelled out the word they heard. Words were produced by two speakers: one with a native English accent (Standard American) and the other with a non-native accent matching that of the participants (native Spanish speaker from Spain). We manipulated orthographic and phonological similarity orthogonally and found that accent did have some effect on both response time and accuracy as well as modulating the effects of similarity. Overall, the non-native accent improved performance, but it did not fully explain why high orthographic similarity items show an inhibitory effect in the auditory modality. Theoretical implications and future directions are discussed.
Chinese English as a Foreign Language Teachers’ Wellbeing and Motivation: The Role of Mindfulness
Authors: Pan Menglai , Liu Jieying
Published: May 2022

Teaching is a career with great instances of anxiety and exhaustion in all stages of education with particular difficulties associated with the attribute of language instruction. The notion of motivation might be a significant fundamental mechanism since demotivated educators are distressed due to the anxious feature of the instructing career. Moreover, educators’ wellbeing has been demonstrated to have a pivotal function in the path of instruction and learners’ success. On the other hand, to mitigate both motivation and wellbeing among teachers, one of the mental traits in this filed, namely, mindfulness can be effective as it is a technique that link to positive effects when used as an administrative strategy for alleviating stress and concern that bring about motivation and wellbeing. As a result, the purpose of the study is to investigate the predictor role of mindfulness on teachers’ motivation and wellbeing. In this study, 577 teachers (235 males and 342 females) Chinese English as a foreign language (EFL) teachers at different colleges, universities, and institutes in 13 provinces among which Jiangsu and Zhejiang province accounted for 26.69%, while other provinces made up 65.86% and 2 municipalities directly under the central government (Beijing and Chongqing; 7.45%). were kindly accepted to participate in the present study, and they answered the three questionnaires, namely, motivation, mindfulness, and wellbeing. The results of the study through a linear regression analysis indicated that teachers’ mindfulness could significantly predict both teachers’ wellbeing and motivation. According to the results, some pedagogical suggestions for the policymakers, educator trainers, materials developers, and language educators are offered. Ultimately, guidance for further studies is proposed to L2 scholars who are interested.
Developing an Integrated Evaluation Model for Physician Comprehensive Workload Tethered to Outpatient Practice: An Empirical Study From China
Authors: Li Dehe , Hu Yinhuan , Liu Sha , Lu Chuntao , Zhang Yeyan , Zhou Jinghan , Li Jiayi , Zhang Zemiao
Published: May 2022

Background

Previous studies, often simply using either objective workload or mental workload as a measure of physician workload in various healthcare settings might have failed to comprehensively reflect the real workload among physicians. Despite this, there is little research that further explores a comprehensive workload evaluation framework with the integration of objective workload and mental workload to describe their comprehensive workload.

Methods

A comprehensive evaluation framework for physician workload was proposed based on the combination of objective workload and task-level mental workload also with the consideration of quality of provided medical services and served patient complexity; and accordingly, an integrated evaluation model for physician comprehensive workload (PCW) tethered to outpatient practice was developed and further applied to perform a PCW analysis using cross-sectional data on outpatient workload of 1,934 physicians mainly from 24 hospitals in 6 provinces in Eastern, Central, and Western China. Multiple linear regression and multinomial logistic regression analyses were established to identify significant factors influencing the PCW.

Results

Overall, the average score of PCW tethered to outpatient practice Chinese physicians experienced was 811.30 (SD=494.98) with concentrating on between 200 and 1,200. Physicians who were female, from Eastern or Western China, and those who worked >60 h per week and longer outpatient hours per week were more likely to experience a higher PCW. 11.2% of participating physicians were identified as very high PCW physicians, compared with 11.6% as low PCW physicians, 45.5% as medium PCW physicians and 30.7% as high PCW physicians. Those who were female, older, from Western China, those who had lower educational levels, lower professional titles and longer working years in the current institution, and those who worked in tertiary A hospitals and Internal or Surgical, and worked >60 h per week and longer outpatient hours per week were more likely to be very high PCW physicians.

Conclusions

Our work has a potential application for comprehensively assessing physician workload tethered to outpatient practice and could provide a solid foundation for hospital managers to further accurately determine and identify physicians with high workload, who would otherwise be missed in either objective workload or mental workload.
The Martian Crustal Magnetic Field
Authors: Mittelholz Anna , Johnson Catherine L.
Published: May 2022

Mars’ crustal magnetic field holds information on the planet’s interior evolution and exterior processes that have modified the crust. Crustal magnetization records an ancient dynamo field that indicates very different interior conditions in the past, possibly linked to the presence of a thicker early atmosphere. Current data sets have provided a wealth of information on the ancient magnetic field, and on the acquisition and modification of magnetization in the crust. However, many puzzles remain regarding the nature and origin of crustal magnetization, and the timing and characteristics of the past dynamo. Here we use recent advances in understanding martian magnetism to highlight open questions, and ways in which they can be addressed through laboratory analysis, modeling and new data sets. Many of the outstanding key issues require data sets that close the gap in spatial resolution between available global satellite and local surface magnetic field measurements. Future missions such as a helicopter, balloon or airplane can provide areal high resolution coverage of the magnetic field, vital to major advances in understanding planetary crustal magnetic fields.
Bone Defects in Tibia Managed by the Bifocal vs. Trifocal Bone Transport Technique: A Retrospective Comparative Study
Authors: Abulaiti Alimujiang , Liu Yanshi , Cai Feiyu , Liu Kai , Abula Abulaiti , Maimaiti Xiayimaierdan , Ren Peng , Yusufu Aihemaitijiang
Published: May 2022

Background

The purpose of this study is to evaluate the clinical effectiveness and determine the differences, if any, between the trifocal bone transport (TFT) technique and the bifocal bone transport (BFT) technique in the reconstruction of long segmental tibial bone defects caused by infection using a monolateral rail external fixator.

Methods

A total of 53 consecutive patients with long segmental tibial bone defects caused by infection and treated by monolateral rail external fixator in our department were retrospectively collected and analyzed from the period January 2013 to April 2019, including 39 males and 14 females with an average age of 38.8 ± 12.4 years (range 19–65 years). Out of these, 32 patients were treated by the BFT technique, and the remaining 21 patients were managed by the TFT technique. The demographic data, operation duration (OD), docking time (DT), external fixation time (EFT), and external fixation index (EFI) were documented and analyzed. Difficulties that occur during the treatment were classified according to Paley. The clinical outcomes were evaluated by following the Association for the Study and Application of the Method of Ilizarov (ASAMI) criteria at the last clinical visit.

Results

All patients achieved an infection-free union finally, and there was no significant difference between the two groups in terms of demographic data and both ASAMI bone and functional scores (p > 0.05). The mean defect size and OD in TFT (9.4 ± 1.5 cm, 161.9 ± 8.9 min) were larger than that in BFT (7.8 ± 1.8 cm, 122.5 ± 11.2 min) (p < 0.05). The mean DT, EFT, and EFI in TFT (65.9 ± 10.8 days, 328.0 ± 57.2 days, 34.8 ± 2.1 days/cm) were all less than those in BFT (96.8 ± 22.6 days, 474.5 ± 103.2 days, 60.8 ± 1.9 days/cm) (p < 0.05). Difficulties and complications were more prevalent in the BFT group than in the TFT group (p < 0.05).

Conclusion

Both the trifocal and BFT techniques achieve satisfactory clinical outcomes in the reconstruction of long segmental tibial bone defects caused by infection using a monolateral rail external fixator. The TFT technique can significantly decrease the DT, EFT, EFI, difficulties, and complications compared with the BFT technique.
Blood, Toil, and Taxoteres: Biological Determinants of Treatment-Induced ctDNA Dynamics for Interpreting Tumor Response
Authors: Boniface Christopher T. , Spellman Paul T.
Published: May 2022

Collection and analysis of circulating tumor DNA (ctDNA) is one of the few methods of liquid biopsy that measures generalizable and tumor specific molecules, and is one of the most promising approaches in assessing the effectiveness of cancer care. Clinical assays that utilize ctDNA are commercially available for the identification of actionable mutations prior to treatment and to assess minimal residual disease after treatment. There is currently no clinical ctDNA assay specifically intended to monitor disease response during treatment, partially due to the complex challenge of understanding the biological sources of ctDNA and the underlying principles that govern its release. Although studies have shown pre- and post-treatment ctDNA levels can be prognostic, there is evidence that early, on-treatment changes in ctDNA levels are more accurate in predicting response. Yet, these results also vary widely among cohorts, cancer type, and treatment, likely due to the driving biology of tumor cell proliferation, cell death, and ctDNA clearance kinetics. To realize the full potential of ctDNA monitoring in cancer care, we may need to reorient our thinking toward the fundamental biological underpinnings of ctDNA release and dissemination from merely seeking convenient clinical correlates.
Human-Origin iPSC-Based Recellularization of Decellularized Whole Rat Livers
Authors: Acun Aylin , Oganesyan Ruben , Jaramillo Maria , Yarmush Martin L. , Uygun Basak E.
Published: May 2022

End-stage liver diseases lead to mortality of millions of patients, as the only treatment available is liver transplantation and donor scarcity means that patients have to wait long periods before receiving a new liver. In order to minimize donor organ scarcity, a promising bioengineering approach is to decellularize livers that do not qualify for transplantation. Through decellularization, these organs can be used as scaffolds for developing new functional organs. In this process, the original cells of the organ are removed and ideally should be replaced by patient-specific cells to eliminate the risk of immune rejection. Induced pluripotent stem cells (iPSCs) are ideal candidates for developing patient-specific organs, yet the maturity and functionality of iPSC-derived cells do not match those of primary cells. In this study, we introduced iPSCs into decellularized rat liver scaffolds prior to the start of differentiation into hepatic lineages to maximize the exposure of iPSCs to native liver matrices. Through exposure to the unique composition and native 3D organization of the liver microenvironment, as well as the more efficient perfusion culture throughout the differentiation process, iPSC differentiation into hepatocyte-like cells was enhanced. The resulting cells showed significantly higher expression of mature hepatocyte markers, including important CYP450 enzymes, along with lower expression of fetal markers, such as AFP. Importantly, the gene expression profile throughout the different stages of differentiation was more similar to native development. Our study shows that the native 3D liver microenvironment has a pivotal role to play in the development of human-origin hepatocyte-like cells with more mature characteristics.
Gasdermin D Deficiency Limits the Transition of Atherosclerotic Plaques to an Inflammatory Phenotype in ApoE Knock-Out Mice
Authors: Puylaert Pauline , Van Praet Melissa , Vaes Frederik , Neutel Cédric H. G. , Roth Lynn , Guns Pieter-Jan , De Meyer Guido R. Y. , Martinet Wim
Published: May 2022

Gasdermin D (GSDMD) is the key executor of pyroptotic cell death. Recent studies suggest that GSDMD-mediated pyroptosis is involved in atherosclerotic plaque destabilization. We report that cleaved GSDMD is expressed in macrophage- and smooth muscle cell-rich areas of human plaques. To determine the effects of GSDMD deficiency on atherogenesis, ApoE−/− Gsdmd−/− (n = 16) and ApoE−/−Gsdmd+/+ (n = 18) mice were fed a western-type diet for 16 weeks. Plaque initiation and formation of stable proximal aortic plaques were not altered. However, plaques in the brachiocephalic artery (representing more advanced lesions compared to aortic plaques) of ApoE−/− Gsdmd−/− mice were significantly smaller (115 ± 18 vs. 186 ± 16 × 103 µm2, p = 0.006) and showed features of increased stability, such as decreased necrotic core area (19 ± 4 vs. 37 ± 7 × 103 µm2, p = 0.03) and increased αSMA/MAC3 ratio (1.6 ± 0.3 vs. 0.7 ± 0.1, p = 0.01), which was also observed in proximal aortic plaques. Interestingly, a significant increase in TUNEL positive cells was observed in brachiocephalic artery plaques from ApoE−/− Gsdmd−/− mice (141 ± 25 vs. 62 ± 8 cells/mm2, p = 0.005), indicating a switch to apoptosis. This switch from pyroptosis to apoptosis was also observed in vitro in Gsdmd−/− macrophages. In conclusion, targeting GSDMD appears to be a promising approach for limiting the transition to an inflammatory, vulnerable plaque phenotype.
Robotic Radical Nephroureterectomy with Bladder Cuff Excision for Upper Tract Urothelial Carcinoma: A Trend Analysis of Utilization and a Comparative Study
Authors: Bae Hoyoung , Chung Jae Hoon , Song Wan , Kang Minyong , Jeon Hwang Gyun , Jeong Byong Chang , Seo Seong Il , Jeon Seong Soo , Lee Hyun Moo , Sung Hyun Hwan
Published: May 2022

Purpose: To compare the perioperative outcomes and oncological results of open, laparoscopic, and robotic radical nephroureterectomy (RNU) in patients with upper tract urothelial carcinoma (UTUC) and to analyze trends in the utilization of RNU. Methods: From 2017 to 2020, the records of 61, 185, and 119 patients who underwent open, laparoscopic, and robotic RNU, respectively, were reviewed. Results: Baseline characteristics were not significantly different among the three groups. Robotic RNU has recently started to increase from 9% in 2017 to 67% in 2021. Operation time, blood loss, length of hospital stay, and 90-day complications were not different between the three groups. The three-year overall survival (OS) rates for open, laparoscopic, and robotic RNU were 91.8%, 90.4%, and 92.1%, respectively (p > 0.05). No differences in the progression-free survival (PFS), cancer-specific survival (CSS), and OS were observed according to the surgical approach in the Kaplan–Meier survival analysis. Multivariate analysis showed that surgical approach was not an independent predictor of PFS, CSS, and OS. Conclusion: The use of robotic RNU in patients with UTUC has been starting to increase and replace open and laparoscopic RNU. Perioperative outcomes, 90-day complications, and oncological outcomes of robotic RNU were not inferior to those of open and laparoscopic RNU.
Combined Application of Patient-Derived Cells and Biomaterials as 3D In Vitro Tumor Models
Authors: Hasbum Asbiel , Karabulut Ozan , Reyes Ruben Edgar , Ricci Claudio , Franchi Alessandro , Danti Serena , Chew Sue Anne
Published: May 2022

Although advances have been made in cancer therapy, cancer remains the second leading cause of death in the U.S. and Europe, and thus efforts to continue to study and discover better treatment methods are ongoing. Three-dimensional (3D) tumor models have shown advantages over bi-dimensional (2D) cultures in evaluating the efficacy of chemotherapy. This commentary aims to highlight the potential of combined application of biomaterials with patient-derived cancer cells as a 3D in vitro model for the study and treatment of cancer patients. Five studies were discussed which demonstrate and provided early evidence to create 3D models with accurate microenvironments that are comparable to in vivo tumors. To date, the use of patient-derived cells for a more personalized approach to healthcare in combination with biomaterials to create a 3D tumor is still relatively new and uncommon for application in clinics. Although highly promising, it is important to acknowledge the current limitations and challenges of developing these innovative in vitro models, including the need for biologists and laboratory technicians to become familiar with biomaterial scaffolds, and the effort for bioengineers to create easy-to-handle scaffolds for routine assessment.
Impact of HER2 Status on Pathological Response after Neoadjuvant Chemotherapy in Early Triple-Negative Breast Cancer
Authors: Domergue Camille , Martin Elodie , Lemarié Camille , Jézéquel Pascal , Frenel Jean-Sebastien , Augereau Paule , Campone Mario , Patsouris Anne
Published: May 2022

Purpose: Investigates the link between HER2 status and histological response after neoadjuvant chemotherapy in patients with early TNBC. Methods: We retrieved clinical and anatomopathological data retrospectively from 449 patients treated for the first time with standard neoadjuvant chemotherapy for early unilateral BC between 2005 and 2020. The primary endpoint was pathological complete response (pCR, i.e., ypT0 ypN0), according to HER2 status. Secondary endpoints included invasive disease-free survival (I-DFS) and overall survival (OS). Results: 437 patients were included, and 121 (27.7%) patients had HER2-low tumours. The pCR rate was not significantly different between the HER2-low group vs. the HER2-0 group (35.7% versus 41.8%, p = 0.284) in either univariate analysis or multivariate analysis adjusted for TNM classification and grade (odds ratio [OR] = 0.70, confidence interval [CI] 95% 0.45–1.08). With a median follow-up of 72.9 months, no significant survival differences were observed between patients with HER2-low tumours vs. patients with HER2-0 tumours in terms of I-DFS (p = 0.487) and OS (p = 0.329). Conclusions: In our cohort, HER2 status was not significantly associated with pCR in a manner consistent with data published recently on TNBC. However, the prognostic impact of HER2-low expression among TNBC patients warrants further evaluation.
Epigenetic-Like Stimulation of Receptor Expression in SSTR2 Transfected HEK293 Cells as a New Therapeutic Strategy
Authors: Kotzerke Joerg , Buesser Dorothee , Naumann Anne , Runge Roswitha , Huebinger Lisa , Kliewer Andrea , Freudenberg Robert , Brogsitter Claudia
Published: May 2022

The aim of the study was to increase the uptake of the SSTR2-targeted radioligand Lu-177-DOTATATE using the DNA methyltransferase inhibitor (DNMTi) 5-aza-2′-deoxycytidine (5-aza-dC) and the histone deacetylase inhibitor (HDACi) valproic acid (VPA). The HEKsst2 and PC3 cells were incubated with variable concentrations of 5-aza-dC and VPA to investigate the uptake of Lu-177-DOTATATE. Cell survival, subsequent to external X-rays (0.6 or 1.2 Gy) and a 24 h incubation with 57.5 or 136 kBq/mL Lu-177-DOTATATE, was investigated via colony formation assay to examine the effect of the epidrugs. In the case of stimulated HEKsst2 cells, the uptake of Lu-177-DOTATATE increased by a factor of 28 in comparison to the unstimulated cells. Further, stimulated HEKsst2 cells demonstrated lower survival fractions (factor 4). The survival fractions of the PC3 cells remained almost unchanged. VPA and 5-aza-dC did not induce changes to the intrinsic radiosensitivity of the cells after X-ray irradiation. Clear stimulatory effects on HEKsst2 cells were demonstrated by increased cell uptake of the radioligand and enhanced SST2 receptor quantity. In conclusion, the investigated approach is suitable to stimulate the somatostatin receptor expression and thus the uptake of Lu-177-DOTATATE, enabling a more efficient treatment for patients with poor response to peptide radionuclide therapy (PRRT).
How Surface-Enhanced Raman Spectroscopy Could Contribute to Medical Diagnoses
Authors: Szaniawska Aleksandra , Mazur Kinga , Kwarta Dominika , Pyrak Edyta , Kudelski Andrzej
Published: May 2022

In the last decade, there has been a rapid increase in the number of surface-enhanced Raman scattering (SERS) spectroscopy applications in medical research. In this article we review some recent, and in our opinion, most interesting and promising applications of SERS spectroscopy in medical diagnostics, including those that permit multiplexing within the range important for clinical samples. We focus on the SERS-based detection of markers of various diseases (or those whose presence significantly increases the chance of developing a given disease), and on drug monitoring. We present selected examples of the SERS detection of particular fragments of DNA or RNA, or of bacteria, viruses, and disease-related proteins. We also describe a very promising and elegant ‘lab-on-chip’ approach used to carry out practical SERS measurements via a pad whose action is similar to that of a pregnancy test. The fundamental theoretical background of SERS spectroscopy, which should allow a better understanding of the operation of the sensors described, is also briefly outlined. We hope that this review article will be useful for researchers planning to enter this fascinating field.
Effects of Calcination Temperature on CO-Sensing Mechanism for NiO-Based Gas Sensors
Authors: Stanoiu Adelina , Ghica Corneliu , Mihalcea Catalina G. , Ghica Daniela , Somacescu Simona , Florea Ovidiu G. , Simion Cristian E.
Published: May 2022

NiO-sensitive materials have been synthesized via the hydrothermal synthesis route and calcined in air at 400 °C and, alternatively, at 500 °C. Structural, morphological, and spectroscopic investigations were involved. As such, the XRD patterns showed a higher crystallinity degree for the NiO calcined at 500 °C. Such an aspect is in line with the XPS data indicating a lower surface hydroxylation relative to NiO calcined at 400 °C. An HRTEM microstructural investigation revealed that the two samples differ essentially at the morphological level, having different sizes of the crystalline nanoparticles, different density of the surface defects, and preferential faceting according to the main crystallographic planes. In order to identify their specific gas-sensing mechanism towards CO exposure under the in-field atmosphere, the simultaneous evaluation of the electrical resistance and contact potential difference was carried out. The results allowed the decoupling of the water physisorption from the chemisorption of the ambient oxygen species. Thus, the specific CO interaction mechanism induced by the calcination temperature of NiO has been highlighted.
Climate Variability Shifting Immigrated Rice Planthoppers in Taiwan
Authors: Huang Shou-Horng , Lai Pau-Yu , Hwang Shaw-Yhi , Borhara Krishna , Huang Wan-Ru , Wang Shih-Yu
Published: May 2022

Rice planthoppers are common insect pests in Taiwan, and they have caused significant damage in the past. The majority of rice planthoppers have seen a drastic decline in their population since the mid-2000s, a trend that has anecdotally attributed to widespread and better pest control, as well as improved rice cultivation management. By analyzing 40 years of the airborne net trap data of rice planthoppers collected in Southwest Taiwan, it was found that the pests’ yearly population, computed with a logarithmic transformation, resembles a signature climate pattern in the global oceans with a robust multi-decadal variability. An ocean temperature-based index derived from the patterns of multi-decadal variability shows a marked resemblance with the population change of common rice planthoppers, with overlapping peaks during the 1990–2010 period. The climate dynamics associated with the regional weather pattern in the vicinity of Taiwan are discussed. Phase reversal of this multi-decadal climate variability in the future may produce favorable climatic conditions for the rice planthopper population to increase back to its historical levels.
A Video-Game-Based Oral Health Intervention in Primary Schools—A Randomised Controlled Trial
Authors: Aljafari Ahmad , ElKarmi Rawan , Nasser Osama , Atef Ala’a , Hosey Marie Therese
Published: May 2022

Background: Poor oral health practices and high levels of dental caries have been reported among children in the developing world. Video games have been successful in promoting oral health in children. The aim of this study was to assess the impact of an oral-health-education video game on children’s dietary knowledge and dietary and toothbrushing practices; Methods: Two Schools in Amman, Jordan were randomly selected and assigned to either intervention or control. Six- to eight-year old children took part. The intervention group played the oral-health-education video game; the control group received no intervention. The groups were compared in terms of changes in: child dietary knowledge, dietary and toothbrushing practices, plaque scores, and parental familiarity with preventive treatments. Data were submitted to statistical analysis with the significance level set at p ≤ 0.05. Results: Two hundred and seventy-eight children took part. Most (92%) had carious teeth. At baseline, children reported having more than one sugary snack a day and only 33% were brushing twice a day. Most parents were unaware of fluoride varnish (66%) or fissure sealants (81%). At follow-up, children in the intervention group had significantly better dietary knowledge, and parents in both groups became more familiar with fluoride varnish. There were no significant changes in children’s plaque scores, toothbrushing and dietary practices, or parental familiarity with fissure sealants in either group. Conclusions: Using an oral-health-education video game improved children’s dietary knowledge. However, future efforts should target children together with parents, and need to be supplemented by wider oral-health-promotion.
Impacts of Overall Financial Development, Access and Depth on Income Inequality
Authors: Mbona Nokulunga
Published: May 2022

There is dense literature on the relationship between financial sector development (FSD) and income inequality. However, most of these studies employ a depth measure of FSD. This study argues that different components of FSD have a heterogenous impact on income inequality. This study first empirically tests the overall impact of FSD on income inequality. Thereafter, I investigate both the linear and nonlinear impact of financial sector development dimensions (depth and access) on income inequality. The study’s novelty lies in using financial access data such as ATM per adult and financial access index and comparing their impact on income inequality versus the impact of financial sector depth (growth in domestic credit) on inequality. Adding to this, fewer studies have investigated the overall impact of FSD. To solve the endogenous problem, the study uses the system General Method of Moments (GMM) on the panel data of 120 countries, from 2004 to 2019. The findings of the study are threefold. Firstly, the study finds that the overall FSD index, individual financial institutions, and market development index all narrow income inequality. Secondly, this study finds that different dimensions of FSD have heterogenous impacts on income inequality, where increased access to financial services reduces income inequality in both linear and nonlinear models. While financial sector depth narrows income inequality in the linear model, the nonlinear model reveals that the Too Much Finance hypothesis holds, as the results confirm a U-shaped relation with income inequality. These results are important for policy decisions concerning financial reforms and income distribution. These results imply that financial sector reforms can be shaped to reduce income inequality by increasing access to credit and through credit policy provisions.
Parsing Long-Term Tree Recruitment, Growth, and Mortality to Identify Hurricane Effects on Structural and Compositional Change in a Tropical Forest
Authors: Zhang Jiaying , Heartsill-Scalley Tamara , Bras Rafael L.
Published: May 2022

After hurricane disturbances in tropical forests, the size structure and species composition are affected by immediate mortality, and subsequent recruitment and individual growth. Often, immediate post-disturbance stand-level data are presented but understanding of the components that affect changes in growth and longer-term responses to forest structure and composition are lacking. To answer questions about how mortality, recruitment, and growth change among successional Plant Functional Types (PFT) through time after a hurricane disturbance, we use long-term census data (1989–2014) collected in the Luquillo Experimental Forest, Puerto Rico. We developed an algorithm to fill missing diameter data from the long-term data set that was collected three months after Hurricane Hugo; and subsequently at five-year intervals. Both the immediate hurricane-induced mortality and subsequent mortality were lower in stems with larger diameters, but varied among successional PFTs Early, Mid, Late, and Palm. Tree growth rates were observed to decrease with time since the hurricane disturbance. Five years after the hurricane, mortality was minimal but then increased gradually with time. In contrast, recruitment was highest five years after the hurricane and then decreased with time. The palm Prestoea montana became the most abundant species in the forest after the hurricane, as it had the lowest immediate hurricane-induced and subsequent mortality, and the highest recruitment. Twenty-five years after the hurricane, the palm and the Late PFT dominate the forest after shifting species composition from pre-hurricane conditions.
Thermosonication of Broccoli Florets Prior to Fermentation Increases Bioactive Components in Fermented Broccoli Puree
Authors: Shokri Sajad , Jegasothy Hema , Hliang Mya Myintzu , Augustin Mary Ann , Terefe Netsanet Shiferaw
Published: May 2022

The aim of this study was to compare the effects of thermosonication (18 kHz at 60 °C for 7 min) pre-treatment with thermal treatment alone (60 °C for 7 min) of broccoli florets prior to pureeing and fermentation on selected bioactive components of fermented broccoli puree. Both thermal and thermosoncation pre-treatments significantly increased the rate of acidification of broccoli puree compared to control untreated broccoli puree, with the time to reach pH 4 being 8.25, 9.9, and 24 h, respectively, for thermally treated, thermosonicated, and control samples. The highest sulforaphane yield of 7268 µmol/kg dry weight (DW) was observed in the thermosonicated samples, followed by 6227 µmol/kg DW and 3180 µmol/kg DW in the thermally treated and untreated samples, respectively. The measurable residual glucoraphanin content was 1642 µmol/kg DW, 1187 µmol/kg DW, and 1047 µmol/kg DW, respectively, in the thermonsonicated, thermally pre-treated, and control fermented samples, indicating that pre-treatment specially by thermosonication increases the extractability of glucoraphanin. The higher sulforaphane yield in the thermosonicated and thermally pre-treated samples could be due to increased extractability and accessibility of glucoraphanin and interaction with myrosinase in addition to the inactivation of epthiospecifier protein (ESP), which directs conversion away from sulforaphane into sulforaphane nitrile.
Hydrogels for Antitumor and Antibacterial Therapy
Authors: Fang Xiuling , Wang Cheng , Zhou Shuwen , Cui Pengfei , Hu Huaanzi , Ni Xinye , Jiang Pengju , Wang Jianhao
Published: May 2022

As a highly absorbent and hydrophobic material with a three-dimensional network structure, hydrogels are widely used in biomedical fields for their excellent biocompatibility, low immunogenicity, adjustable physicochemical properties, ability to encapsulate a variety of drugs, controllability, and degradability. Hydrogels can be used not only for wound dressings and tissue repair, but also as drug carriers for the treatment of tumors. As multifunctional hydrogels are the focus for many researchers, this review focuses on hydrogels for antitumor therapy, hydrogels for antibacterial therapy, and hydrogels for co-use in tumor therapy and bacterial infection. We highlighted the advantages and representative applications of hydrogels in these fields and also outlined the shortages and future orientations of this useful tool, which might give inspirations for future studies.
Evaluation of Structural Behavior in the Process Dynamics of Oleogel-Based Tender Dough Products
Authors: Tanislav Anda E. , Pușcaș Andreea , Păucean Adriana , Mureșan Andruța E. , Semeniuc Cristina A. , Mureșan Vlad , Mudura Elena
Published: May 2022

The current trend is represented by replacing solid fats with structured liquid oil while maintaining the plastic properties of food products. In this study, the behavior of refined sunflower oil structured with various agents (carnauba wax-CRW, β-sitosterol:beeswax-BS:BW, β-sitosterol:lecithin-BS:LEC, and glycerol monostearate-GM) was evaluated in the process dynamics of oleogel-based tender dough products. The oleogel with the mixture of β-sitosterol:beeswax (OG_BS:BW) displayed the highest capacity to retain oil inside the matrix with a percentage of oil loss as low as 0.05% and also had a significantly higher hardness (6.37 N) than the reference, a commercial margarine (MR—3.58 N). During cooling from 90 to 4 °C, the increase in oleogel’ viscosity results from oleogelator’s liquid–solid phase transition. As demonstrated by the frequency sweeps performed, storage modulus G′ was higher than loss modulus G″, no cross-over points were observed, and the strongest gel network was for the oleogel with glycerol monostearate (OG_GM). Regarding the dough, the sample prepared using the oleogel with carnauba wax (D_CRW) showed the strongest hardness (92.49 N) compared to the reference (D_MR—21.80 N). All the oleogel-containing doughs had elastic solid-like behavior. The samples with margarine (D_MR) and the mixture of β-sitosterol:lecithin (D_BS:LEC) presented the lowest value of both moduli of G’ and G” during the frequency sweep. The biscuits formulated with commercial margarine (B_MR) registered a hardness of 28.74 N. Samples with oleogels showed a specific tenderness for tender dough products, thus being suitable for this type of product (11.22–20.97 N).
Crisaborole Loaded Nanoemulsion Based Chitosan Gel: Formulation, Physicochemical Characterization and Wound Healing Studies
Authors: Ansari Mohd Nazam , Soliman Gamal A. , Rehman Najeeb Ur , Anwer Md. Khalid
Published: May 2022

The development of an effective gel capable of treating eczema remains a challenge in medicine. Because of its greater retention in the affected area, good absorption of wound exudates, and induction of cell growth, nanogel is widely investigated as a topical preparation. Chitosan gel based on nanoemulsions has received much attention for its use in wound healing. In this study, four formulae (CRB-NE1-CRB-NE4) of crisaborole-loaded nanoemulsions (CRB-NEs) were developed using lauroglycol 90 as an oil, Tween-80 as a surfactant, and transcutol-HP (THP) as a co-surfactant. The prepared NEs (CRB-NE1-CRB-NE4) were evaluated for their physicochemical properties. Based on vesicle size (64.5 ± 5.3 nm), polydispersity index (PDI) (0.202 ± 0.06), zeta potential (ZP, −36.3 ± 4.16 mV), refractive index (RI, 1.332 ± 0.03), and percent transmittance (% T, 99.8 ± 0.12) was optimized and further incorporated into chitosan (2%, w/w) polymeric gels. The CRB-NE1-loaded chitosan gel was then evaluated for its drug content, spreadability, in-vitro release, flux, wound healing, and anti-inflammatory studies. The CRB-NE1-loaded chitosan gel exhibited a flux of 0.211 mg/cm2/h, a drug release of 74.45 ± 5.4% CRB released in 24 h with a Korsmeyer-Peppas mechanism release behavior. The CRB-NE1-loaded gel exhibited promising wound healing and anti-inflammatory activities.
m1A RNA Modification in Gene Expression Regulation
Authors: Jin Hao , Huo Chunxiao , Zhou Tianhua , Xie Shanshan
Published: May 2022

N1-methyladenosine (m1A) is a prevalent and reversible post-transcriptional RNA modification that decorates tRNA, rRNA and mRNA. Recent studies based on technical advances in analytical chemistry and high-throughput sequencing methods have revealed the crucial roles of m1A RNA modification in gene regulation and biological processes. In this review, we focus on progress in the study of m1A methyltransferases, m1A demethylases and m1A-dependent RNA-binding proteins and highlight the biological mechanisms and functions of m1A RNA modification, as well as its association with human disease. We also summarize the current understanding of detection approaches for m1A RNA modification.