bluuebunny/crossref_metadata_2025 huggingface dataset:
The Downregulation of Both Giant HERCs, HERC1 and HERC2, Is an Unambiguous Feature of Chronic Myeloid Leukemia, and HERC1 Levels Are Associated with Leukemic Cell Differentiation
Authors: Ali Muhammad Shahzad , Magnati Stefano , Panuzzo Cristina , Cilloni Daniela , Saglio Giuseppe , Pergolizzi Barbara , Bracco Enrico
Published: January 2022

Large HERC E3 ubiquitin ligase family members, HERC1 and HERC2, are staggeringly complex proteins that can intervene in a wide range of biological processes, such as cell proliferation, DNA repair, neurodevelopment, and inflammation. Therefore, mutations or dysregulation of large HERCs is associated with neurological disorders, DNA repair defects, and cancer. Though their role in solid tumors started to be investigated some years ago, our knowledge about HERCs in non-solid neoplasm is greatly lagging behind. Chronic Myeloid Leukemia (CML) is a model onco-hematological disorder because of its unique and unambiguous relation between genotype and phenotype due to a single genetic alteration. In the present study, we ascertained that the presence of the BCR-ABL fusion gene was inversely associated with the expression of the HERC1 and HERC2 genes. Upon the achievement of remission, both HERC1 and HERC2 mRNAs raised again to levels comparable to those of the healthy donors. Additionally, our survey unveiled that their gene expression is sensitive to different Tyrosine Kinases Inhibitors (TKIs) in a time-dependent fashion. Interestingly, for the first time, we also observed a differential HERC1 expression when the leukemic cell lines were induced to differentiate towards different lineages revealing that HERC1 protein expression is associated with the differentiation process in a lineage-specific manner. Taken together, our findings suggest that HERC1 might act as a novel potential player in blood cell differentiation. Overall, we believe that our results are beneficial to initiate exploring the role/s of large HERCs in non-solid neoplasms.
Predictive Validity of Motor Fitness and Flexibility Tests in Adults and Older Adults: A Systematic Review
Authors: Marín-Jiménez Nuria , Cruz-León Carolina , Perez-Bey Alejandro , Conde-Caveda Julio , Grao-Cruces Alberto , Aparicio Virginia A. , Castro-Piñero José , Cuenca-García Magdalena
Published: January 2022

Motor fitness and flexibility have been linked to several health issues. We aimed to investigate the predictive validity of motor fitness and flexibility tests in relation to health outcomes in adults and older adults. Web of Science and PubMed databases were screened for studies published from inception to November 2020. Two authors systematically searched, evaluated, and extracted data from identified original studies and systematic reviews/meta-analysis. Three levels of evidence were constructed: strong, moderate, and limited/inconclusive evidence. In total, 1182 studies were identified, and 70 studies and 6 systematic reviews/meta-analysis were summarized. Strong evidence indicated that (i) slower gait speed predicts falls and institutionalization/hospitalization in adults over 60 years old, cognitive decline/impairment over 55 years old, mobility disability over 50 years old, disability in instrumental activities of daily living (IADL) over 54 years old, cardiovascular disease risk over 45 years old, and all-cause mortality over 35 years old; (ii) impaired balance predicts falls and disability in IADL/mobility disability in adults over 40 years old and all-cause mortality over 53 years old; (iii) worse timed up&go test (TUG) predicts falls and fear of falling over 40 years old. Evidence supports that slower gait speed, impaired balance, and worse TUG performance are significantly associated with an increased risk of adverse health outcomes in adults.
The Best of Two Different Visual Instructions in Improving Precision Ball-Throwing and Standing Long Jump Performances in Primary School Children
Authors: Sorgente Vincenzo , Cohen Erez James , Bravi Riccardo , Minciacchi Diego
Published: January 2022

Two observational learning approaches have been shown to be successful in improving children’s motor performances: one is “technique-focused”, another is “goal-focused”. In this study, we sought to compare the effectiveness of these two strategies, thus testing for the more efficient method of observational learning to enhance motor skills in primary school children. To this end, two experiments were designed. Experiment 1 involved a precision ball throwing task. Experiment 2 involved a standing long jump task. A total of 792 subjects (aged 6–11) participated in this study and were divided into technique-focus (Experiment 1 n = 200; Experiment 2 n = 66), goal-focus (Experiment 1 n = 195; Experiment 2 n = 68), and control groups (Experiment 1 n = 199; Experiment 2 n = 64). The experiments were divided into pretest, practice, and retention phases. During the practice phase, the technique-focus and goal-focus groups were given different visual instructions on how to perform the task. The results showed that children aged 10–11 belonging to the technique-focus group performed significantly better in the practice phase than both the goal-focus and the control group (p < 0.001), but only for the precision ball throwing task. These findings could be useful for training adaptation in the context of motor learning and skills acquisition.
Experimental Investigation on Deterioration Mechanisms of Concrete under Tensile Stress-Chloride Ion-Carbon Dioxide Multiple Corrosion Environment
Authors: Chen Xiangsheng , Shen Jun
Published: January 2022

The adverse effects of a hostile marine environment on concrete structures inevitably result in great economic loss and may contribute to catastrophic failure. There is limited information on the durability of concrete in a tensile stress-chloride ion-carbon dioxide (TCC) multiple-corrosion environment. The objective of this study is to explore the impact of a TCC multiple-corrosion environment on concrete considering three coupled factors of compressive strength, Cl− penetration, and carbonation. Dry–wet cycle tests were conducted to determine the strength degradation and Cl− penetration concentration of concrete in a hostile multiple-corrosion marine environment. The results show that the effects of water-soluble chloride ions (Cl−), carbon dioxide (CO2), and tensile stress on concrete are not a simple superposition, but involve obvious interaction. The compressive strength of a concrete specimen first increases and then decreases in chlorine salt-carbon tests. The Cl− concentration and tensile stress affect the carbonation depth of concrete, which increases with an increase in Cl− concentration, and with the application of tensile stress. The Cl− concentration has an obvious effect on the carbonation depth. In addition to experimental observations, a stepwise regression equation was established based on the multiple linear regression theory. A correlation analysis considering different factors was conducted to reflect the corrosion results more directly.
Ship Type Recognition Based on Ship Navigating Trajectory and Convolutional Neural Network
Authors: Yang Tianyu , Wang Xin , Liu Zhengjiang
Published: January 2022

With the aim to solve the problem of missing or tampering of ship type information in AIS information, in this paper, a novel ship type recognition scheme based on ship navigating trajectory and convolutional neural network (CNN) is proposed. Firstly, according to speed and acceleration of the ship, three ship navigating situations, i.e., static, normal navigation and maneuvering, are integrated into the process of trajectory images generation in the form of pixels. Then, three kinds of modular network structures with different depths are trained and optimized to determine the appropriate convolutional neural network structure. In the validation phase of the model, a large amount of verified data with a time span of one month was used, covering a variety of water conditions including open water, ports, rivers and lakes. Following this approach, a kind of CNN scheme which can be directly used to identify ship types in a wide range of waters is proposed. This scheme can be used to judge the ship type when the static information is completely missing and to test the data when the ship type information is partially missing.
Regulation of 17α-Hydroxyprogesterone Production during Induced Oocyte Maturation and Ovulation in Amur Sturgeon (Acipenser schrenckii)
Authors: Hasegawa Yuya , Surugaya Ryohei , Adachi Shinji , Ijiri Shigeho
Published: January 2022

In several teleosts, 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) has been identified as a maturation-inducing steroid. DHP is synthesized from 17α-hydroxyprogesterone (17OHP) by 17β-hydroxysteroid dehydrogenase type 12-like (hsd17b12L). Along with 3β-hydroxysteroid dehydrogenase/Δ5-4 isomerase (3β-HSD), 17α-hydroxylase and C17-20 lyase are associated with 17OHP production. This study aimed to determine the roles of Amur sturgeon hsd3b, P450c17-I (cyp17a1), and P450c17-II (cyp17a2) in 17OHP production and to examine their enzyme activity and mRNA expression pattern during oocyte maturation. In the sturgeons used in this study, hsd3b encoded 3β-HSD, cyp17a1 catalyzed 17α-hydroxylase production with C17-20 lyase activity, and cyp17a2 processed 17α-hydroxylase activity alone. In the ovarian follicles of individuals that underwent induced ovulation, hsd3b mRNA levels increased rapidly, cyp17a1 expression was downregulated, and cyp17a2 expression was upregulated during oocyte maturation. Finally, an in vitro study revealed that salmon pituitary extract (SPE) stimulation rapidly induced hsd3b expression, whereas cyp17a1 expression was downregulated. In vitro, cyp17a2 expression did not rapidly increase with SPE stimulation. This rapid upregulation of hsd3b during oocyte maturation was first observed in teleosts. It was suggested that hsd17b12L expression is upregulated after 17OHP production, which is regulated by hsd3b, cyp17a1, and cyp17a2, resulting in DHP production.
Local Environmental Conditions Promote High Turnover Diversity of Benthic Deep-Sea Fungi in the Ross Sea (Antarctica)
Authors: Barone Giulio , Corinaldesi Cinzia , Rastelli Eugenio , Tangherlini Michael , Varrella Stefano , Danovaro Roberto , Dell’Anno Antonio
Published: January 2022

Fungi are a ubiquitous component of marine systems, but their quantitative relevance, biodiversity and ecological role in benthic deep-sea ecosystems remain largely unexplored. In this study, we investigated fungal abundance, diversity and assemblage composition in two benthic deep-sea sites of the Ross Sea (Southern Ocean, Antarctica), characterized by different environmental conditions (i.e., temperature, salinity, trophic availability). Our results indicate that fungal abundance (estimated as the number of 18S rDNA copies g−1) varied by almost one order of magnitude between the two benthic sites, consistently with changes in sediment characteristics and trophic availability. The highest fungal richness (in terms of Amplicon Sequence Variants−ASVs) was encountered in the sediments characterized by the highest organic matter content, indicating potential control of trophic availability on fungal diversity. The composition of fungal assemblages was highly diverse between sites and within each site (similarity less than 10%), suggesting that differences in environmental and ecological characteristics occurring even at a small spatial scale can promote high turnover diversity. Overall, this study provides new insights on the factors influencing the abundance and diversity of benthic deep-sea fungi inhabiting the Ross Sea, and also paves the way for a better understanding of the potential responses of benthic deep-sea fungi inhabiting Antarctic ecosystems in light of current and future climate changes.
In Silico Predictions of Ecological Plasticity Mediated by Protein Family Expansions in Early-Diverging Fungi
Authors: Orłowska Małgorzata , Muszewska Anna
Published: January 2022

Early-diverging fungi (EDF) are ubiquitous and versatile. Their diversity is reflected in their genome sizes and complexity. For instance, multiple protein families have been reported to expand or disappear either in particular genomes or even whole lineages. The most commonly mentioned are CAZymes (carbohydrate-active enzymes), peptidases and transporters that serve multiple biological roles connected to, e.g., metabolism and nutrients intake. In order to study the link between ecology and its genomic underpinnings in a more comprehensive manner, we carried out a systematic in silico survey of protein family expansions and losses among EDF with diverse lifestyles. We found that 86 protein families are represented differently according to EDF ecological features (assessed by median count differences). Among these there are 19 families of proteases, 43 CAZymes and 24 transporters. Some of these protein families have been recognized before as serine and metallopeptidases, cellulases and other nutrition-related enzymes. Other clearly pronounced differences refer to cell wall remodelling and glycosylation. We hypothesize that these protein families altogether define the preliminary fungal adaptasome. However, our findings need experimental validation. Many of the protein families have never been characterized in fungi and are discussed in the light of fungal ecology for the first time.
Multi-Omics Profiling Approach to Asthma: An Evolving Paradigm
Authors: Gautam Yadu , Johansson Elisabet , Mersha Tesfaye B.
Published: January 2022

Asthma is a complex multifactorial and heterogeneous respiratory disease. Although genetics is a strong risk factor of asthma, external and internal exposures and their interactions with genetic factors also play important roles in the pathophysiology of asthma. Over the past decades, the application of high-throughput omics approaches has emerged and been applied to the field of asthma research for screening biomarkers such as genes, transcript, proteins, and metabolites in an unbiased fashion. Leveraging large-scale studies representative of diverse population-based omics data and integrating with clinical data has led to better profiling of asthma risk. Yet, to date, no omic-driven endotypes have been translated into clinical practice and management of asthma. In this article, we provide an overview of the current status of omics studies of asthma, namely, genomics, transcriptomics, epigenomics, proteomics, exposomics, and metabolomics. The current development of the multi-omics integrations of asthma is also briefly discussed. Biomarker discovery following multi-omics profiling could be challenging but useful for better disease phenotyping and endotyping that can translate into advances in asthma management and clinical care, ultimately leading to successful precision medicine approaches.
Targeted Therapy in Leukaemia, Lymphoma and Myeloma
Authors: Opat Stephen Samuel
Published: January 2022

Historically, most advances in cancer therapy have been pioneered by clinicians managing the blood diseases [...]
New Translational Trends in Personalized Medicine: Autologous Peripheral Blood Stem Cells and Plasma for COVID-19 Patient
Authors: Balzanelli Mario Giosuè , Distratis Pietro , Lazzaro Rita , D’Ettorre Ernesto , Nico Andrea , Inchingolo Francesco , Dipalma Gianna , Tomassone Diego , Serlenga Emilio Maria , Dalagni Giancarlo , Ballini Andrea , Nguyen Kieu Cao Diem , Isacco Ciro Gargiulo
Published: January 2022

The COVID-19 pandemic, caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), still remains a severe threat. At the time of writing this paper, the second infectious wave has caused more than 280,000 deaths all over the world. Italy was one of the first countries involved, with more than 200,000 people reported as infected and 30,000 deaths. There are no specific treatments for COVID-19 and the vaccine still remains somehow inconclusive. The world health community is trying to define and share therapeutic protocols in early and advanced clinical stages. However, numbers remain critical with a serious disease rate of 14%, ending with sepsis, acute respiratory distress syndrome (ARDS), multiple organ failure (MOF) and vascular and thromboembolic findings. The mortality rate was estimated within 2–3%, and more than double that for individuals over 65 years old; almost one patient in three dies in the Intensive Care Unit (ICU). Efforts for effective solutions are underway with multiple lines of investigations, and health authorities have reported success treating infected patients with donated plasma from survivors of the illness, the proposed benefit being protective antibodies formed by the survivors. Plasma transfusion, blood and stem cells, either autologous or allograft transplantation, are not novel therapies, and in this short paper, we propose therapeutic autologous plasma and peripheral blood stem cells as a possible treatment for fulminant COVID-19 infection.
Credit Risk in G20 Nations: A Comparative Analysis in International Finance Using Option-Adjusted-Spreads
Authors: Boliari Natalia , Topyan Kudret
Published: January 2022

Corporate bond yields are the manifestation of the cost of financing for private firms, and if properly evaluated, they provide researchers with valuable risk information. Within this context, this work is the first study producing corporate yield spreads for all S&P-rated bonds of G20 nations to explain their comparative riskiness. The option-adjusted spread analysis is an advanced method that enables us to compare the bonds with embedded options and different cash flow characteristics. For securities with embedded options, the volatility in the interest rates plays a role in ascertaining whether the option is going to be invoked or not. Therefore, researchers need a spread that, when added to all the forward rates on the tree, will make the theoretical value equal to the market price. The spread that satisfies this condition is called the option-adjusted spread, since it considers the option embedded into the issue. Ultimately, this work investigates the credit risk differentials of S&P rated outstanding bonds issued by the G20 nations to provide international finance professionals with option-adjusted corporate yield spreads showing the credit risk attributable to debt instruments. Detailed results computed using OAS methodology are presented in tables and used to answer the six vital credit-risk-related questions introduced in the introduction.
Simulating Multi-Asset Classes Prices Using Wasserstein Generative Adversarial Network: A Study of Stocks, Futures and Cryptocurrency
Authors: Han Feng , Ma Xiaojuan , Zhang Jiheng
Published: January 2022

Financial data are expensive and highly sensitive with limited access. We aim to generate abundant datasets given the original prices while preserving the original statistical features. We introduce the Wasserstein Generative Adversarial Network with Gradient Penalty (WGAN-GP) into the field of the stock market, futures market and cryptocurrency market. We train our model on various datasets, including the Hong Kong stock market, Hang Seng Index Composite stocks, precious metal futures contracts listed on the Chicago Mercantile Exchange and Japan Exchange Group, and cryptocurrency spots and perpetual contracts on Binance at various minute-level intervals. We quantify the difference of generated results (836,280 data points) and original data by MAE, MSE, RMSE and K-S distances. Results show that WGAN-GP can simulate assets prices and show the potential of a market simulator for trading analysis. We might be the first to look into multi-asset classes in a systematic approach with minute intervals across stocks, futures and cryptocurrency markets. We also contribute to quantitative analysis methodology for generated and original price data quality.
The Capitalist Spirit and Endogenous Growth
Authors: Kumar Ronald , Stauvermann Peter , Wernitz Frank
Published: January 2022

The aim of the study is to investigate the influence of the capitalist spirit in conjunction with the distribution of income on economic growth. The capitalist spirit is represented by the fact that savings rates increase with increasing relative income. We extend an endogenous AK growth model in an overlapping generational framework by implementing imperfect competition and Cournot competition. Using this model, we investigate the influence of profits on the intra- and inter-generational distributions of income and economic growth. While increasing incomes lead to a more unequal intra-generational distribution and to a redistribution of income from the old to the young generation, the impact on economic growth is in general ambiguous, although under specific assumptions it becomes positive. Furthermore, the model shows that increasing market power of firms is associated with declining labor and capital shares, declining interest rates, and an increased wealth-to-income ratio.
Towards a Lightweight Intrusion Detection Framework for In-Vehicle Networks
Authors: Basavaraj Dheeraj , Tayeb Shahab
Published: January 2022

With the emergence of networked devices, from the Internet of Things (IoT) nodes and cellular phones to vehicles connected to the Internet, there has been an ever-growing expansion of attack surfaces in the Internet of Vehicles (IoV). In the past decade, there has been a rapid growth in the automotive industry as network-enabled and electronic devices are now integral parts of vehicular ecosystems. These include the development of automobile technologies, namely, Connected and Autonomous Vehicles (CAV) and electric vehicles. Attacks on IoV may lead to malfunctioning of Electronic Control Unit (ECU), brakes, control steering issues, and door lock issues that can be fatal in CAV. To mitigate these risks, there is need for a lightweight model to identify attacks on vehicular systems. In this article, an efficient model of an Intrusion Detection System (IDS) is developed to detect anomalies in the vehicular system. The dataset used in this study is an In-Vehicle Network (IVN) communication protocol, i.e., Control Area Network (CAN) dataset generated in a real-time environment. The model classifies different types of attacks on vehicles into reconnaissance, Denial of Service (DoS), and fuzzing attacks. Experimentation with performance metrics of accuracy, precision, recall, and F-1 score are compared across a variety of classification models. The results demonstrate that the proposed model outperforms other classification models.
Ecological Efficiency of Urban Industrial Land in Metropolitan Areas: Evidence from China
Authors: Li Lei , Pan Chenzi , Ling Shuai , Li Mingqi
Published: January 2022

Industrial land is an indispensable strategic resource in urban development that plays an indispensable role in ensuring the industrial space of urban construction and development. Measuring and analyzing the eco-efficiency of industrial land utilization (ECILU) can provide insights into how to maximize the input–output ratio of industrial land and ensure the sustainable development of land resources and economies. Based on the undesirable output slacks-based measure (SBM) model, choosing land, capital, and labor as input indicators, and the industrial added value and carbon emissions as desirable and undesirable output indicators, this study measured the ECILUs in 78 cities and 13 metropolitan areas in four Chinese major economic zones from 2007 to 2018, analyzed their spatial and temporal evolution characteristics and regional differences, and constructed a Tobit regression model to test the influence mechanism of each variable on the ECILUs in different regions. This has important theoretical and practical significance for the Chinese government in formulating relevant policies and realizing the green utilization of urban land in the future. Empirical results showed that the ECILUs in most cities were low and that the differences between regions were large. The ECILU in the Western Economic Zone was relatively high, followed by the Eastern, Central, and Northeastern Economic Zones. According to the ECILU value and urban synergy degree of each metropolitan area, this study divided the 13 metropolitan areas into four categories. The regression analysis results showed that the variables had different effects on the ECILUs of all cities and the four economic zones in China. It is suggested that all economic zones should reinforce the optimization of industrial structure, control industrial pollutant discharge, and solve the phenomenon of labor surplus. The Eastern Zone should maintain the growth of its economy while focusing on soil quality. The Central Zone should focus on the efficient use of infrastructure, and the Western, Northeastern, and Central Zones should balance the green coverage area and the industrial land area to ensure the efficient use of urban industrial land.
Mining Industry of the Republic of Sakha (Yakutia) and Problems of Environmental and Social Security of Indigenous Peoples
Authors: Burtseva Evdokia , Sleptsov Anatoliy , Bysyina Anna , Fedorova Alla , Dyachkovski Gavril , Pavlova Alevtina
Published: January 2022

The Republic of Sakha (Yakutia; RS(Y)) is located in the northeast of Siberia (Russia) in the basins of the Lena, Yana, and Indigirka rivers, in the lower reaches of the Kolyma River. Yakutia is an industrial–agrarian republic with a developed mining, fuel, and energy industry. Indigenous peoples live mainly in the Arctic regions, where the large-scale development of mineral resources is planned, and South Yakutia, where the mining industry is well developed. The aim of this study is the development of methodological approaches to assessing the impact of the mining industry on the natural environment and the social sphere in the places of residence and traditional economic activities of the indigenous peoples of the North. We used the results of research work (R&D), materials of expeditionary work, and regulatory documents of the Republic of Sakha (Yakutia) and the Russian Federation (RF). The state of the environment (ES) was assessed on the basis of the analysis of indicators for three areas: (a) anthropogenic load, (b) environmental and social consequences, and (c) resistance of natural complexes to technogenic impacts. In total, 22 indicators were used for the 3 areas, for example, population density, person/km2; the volume of extraction of rock mass, million m3; and emissions, t/year. To bring dissimilar indicators into comparable ones, we used a methodological approach with the use of the social risk index (SRI). In Arctic regions (mainly agricultural), the ES is in a favorable and relatively favorable state: SRI 0.61–0.70; in the central regions (mainly agricultural), it is satisfactory and relatively satisfactory: SRI 0.71–1.0; in the southern and western regions with a developed mining industry, it is relatively tense and tense: SRI 1.01–3.0. An extremely tense state of environmental conditions has developed in the city of Yakutsk: SRI ≥ 3. Generally, the deterioration of the environmental situation and vital activity of the indigenous peoples in investigated Arctic region correlated with the impact of the mining industry.
Agricultural Transformation and Its Impact on Ecosystem Services and Human Well-Being in Peri-Urban Areas: The Case of Xi’an, China
Authors: Zhou Zhongxue , Liu Xiaofang , Zheng Bijun , Robinson Guy M. , Song Bingjie
Published: January 2022

There is a gap in understanding the relationships between the transformation of agricultural landscapes, ecosystem services and human well-being in the peri-urban fringe of major cities worldwide. In this paper, we use semi-structured interviews, perception surveys, social surveys and field mapping to examine linkages between agricultural and landscape transition, ecosystem services and human well-being in five sample villages in Xi’an metropolitan zone, China. The results indicate that: (1) Agricultural change has increased landscape fragmentation, with a shift from grain to more profitable horticulture and nursery production. The farming system is more diversified and exhibits a multifunctional character. (2) This transformation has had a significant impact on the character of the agroecosystem. (3) The agricultural transformation towards greater multifunctionality has increased the supply of ecosystem services, including tourism-related activities, potentially improving human well-being. (4) Different combinations of activities in the sample villages were evaluated with respect to a well-being index, indicating the importance of combining horticulture and tourism. (5) Linkages identified between agricultural transformation, ecosystem services and human well-being may have significant implications for potential approaches within future studies.
Spatial Heterogeneity of Driving Factors of Wind Erosion Prevention Services in Northern China by Large-Scale Human Land-Use Management
Authors: Ma Jinfeng , Li Ruonan , Yang Yanzheng , Hai Yue , Han Tian , Zheng Hua
Published: January 2022

Large-scale human land-use management is an effective method for ecosystem restoration and wind erosion prevention service (WEPS) improvement. However, the spatial differences of driving factors and the feedback in subsequent management have received less attention. This study analysed the temporal and spatial changes in the WEPS in northern China from 2000 to 2015, classified the driving modes between the WEPS and environmental factors, distinguished the main driving factors, and proposed suggestions for successive projects. The results showed that, compared with 2000, the amount of WEPSs in 2015 increased by 12.60%, and forest and grassland in the WEPS-increased area was 1.34 times that in the declining area. There were east–west differences in the driving mechanism of WEPS improvement. In addition to climatic and topographic factors, the western division was mainly affected by changes in vegetation quality, whereas the eastern division was affected by the combined influence of vegetation quality and quantity. This study shows the necessity of land-use management and project zoning policies, and provides a reference for policy formulation and management of large-scale ecological projects.
The Influence of New Agricultural Business Entities on Farmers’ Employment Decision
Authors: Cheng Lingjuan , Cui Yilin , Duan Kaifeng , Zou Wei
Published: January 2022

The purpose of this article is to explore the influence of new agricultural business entities on farmers’ employment decision and provide reference for improving policies related to new agricultural business entities and farmers’ employment. This paper constructs a theoretical analysis framework of “new agricultural business entities—land transfer and purchase of agricultural socialized services—farmers’ employment decision”, and then empirically tests the impact of new agricultural business entities on farmers’ employment decision by combining the analysis methods of the benchmark regression, propensity score matching and mediation effects. The research shows that: (1) New agricultural business entities are beneficial for promoting farmers’ employment decision. (2) Renting out land and the purchase of agricultural socialized services have a positive and partially mediating effect between the new agricultural business entities and farmers’ employment decision, and the mediating effects of the two paths account for 7.12% and 6.25% of the total effects, respectively. (3) In addition to key variables, personal characteristics of decision-making, family characteristics and production and management characteristics are also key factors that affect farmers’ employment decision. (4) The new agricultural business entities increase the probability of farmers’ employment (with legal contract) and entrepreneurship, and reduce the idle labor force in rural areas. Finally, this study proposes some policy recommendations including establishing a perfect farmland transfer market, developing rural industry properly and improving agricultural socialized service systems.
Scalar and Counterfactual Approximatives: Investigating Heritage Greek in the USA and Germany
Authors: Oikonomou Despina , Rizou Vasiliki , Bondarenko Daniil , Özsoy Onur , Alexiadou Artemis
Published: January 2022

Approximative constructions present special interest for acquisition due to the counterfactual and scalar inferences they give rise to. In this paper we investigate the acquisition of Greek approximatives by heritage speakers in Germany and the USA. We show that while in English and German there is a single lexical item encoding counterfactuality and scalarity, in Greek there are two lexical items which, as we show, have different interpretations. In view of this difference, we test whether the crosslinguistic differences and the interface nature of approximative constructions affect their representation in heritage language. We present a production study and a comprehension study of approximative constructions. Our findings suggest that the two heritage groups do not diverge from the monolingual group in the domain of approximative constructions.
Thrombotic Thrombocytopenic Purpura in Interferon Beta-1a-Treated Patient Diagnosed with Relapsing-Remitting Multiple Sclerosis: A Case Report
Authors: Plesa Cristina-Florentina , Chitimus Diana Maria , Sirbu Carmen Adella , Țânțu Monica Marilena , Ghinescu Minerva Claudia , Anghel Daniela , Ionita-Radu Florentina
Published: January 2022

Background: Secondary thrombotic thrombocytopenic purpura (TTP) due to interferon beta-1a intramuscular (im) treatment is an uncommon adverse effect with only a few cases in multiple sclerosis patients reported worldwide. TTP together with haemolytic uremic syndrome (HUS) are classic forms of thrombotic microangiopathy, characterized by small-vessel platelet micro-thrombi that manifest clinically in a similar manner. Most common signs and symptoms include bruises and ecchymosis, neurologic symptoms and renal impairment. Interferon beta-1a represents one of the first-line therapies for relapsing-remitting multiple sclerosis due to its accessibility and efficacy. Case presentation: A 36-year-old woman who was previously diagnosed with relapsing-remitting multiple sclerosis had received weekly intramuscular injections with beta-interferon-1a (Avonex 30 mcg). After 9 months of treatment, she presented bruises and ecchymosis on her limbs and torso, epistaxis, gingival bleeding aggravated within 48 h and a persistent headache that was non-responsive to common analgesics. Haematology tests revealed typical results for thrombotic microangiopathy, including severe thrombocytopenia (4000/mm3) and microangiopathic haemolytic anaemia with frequent schistocytes on the peripheral blood smear. Once the beta-interferon administration was ceased and upon the initiation of methylprednisolone, the symptoms remitted. Conclusions: In this case study, we portrayed the particular association between the remission phase of multiple sclerosis and the violent onset of interferon-induced thrombotic thrombocytopenic purpura.
Assessment of a Novel Fixation Method of a Frameless Intrauterine Contraceptive Device Inserted during Cesarean Delivery as a Means of Preventing Displacements and Expulsions: A Prospective Observational Study
Authors: Kutlucan Hazal , Karabacak Recep Onur , De Buyser Stefanie , Erdem Ahmet , Bozkurt Nuray , Demirdağ Erhan , Wildemeersch Dirk
Published: January 2022

The primary objective of this study was to assess the novel fixation method of a frameless copper-releasing intrauterine device inserted following placental delivery during cesarean section and analyze its impact in reducing device displacement and expulsion during and after uterine involution. We hypothesized that the dual-anchoring technique could reduce the risk of intrauterine device displacement and expulsion during and after the uterine involution. The study was conducted at the Gazi University Medicine Faculty Hospital in Ankara, Turkey. Twenty-one pregnant women were enrolled. Insertion was performed following placental removal. To confirm the proper placement and good retention of the device, the distance between the fundal serosa (S) and device anchor knot (A) was measured (S–A) during follow-ups, by ultrasound. There were significant differences in the S–A, as observed by ultrasound at discharge and at 6 weeks post-delivery, which is consistent with the tissue contractions associated with uterine involution. Notwithstanding the uterine involution, no device displacements or expulsions occurred, which indicated a good retention of the frameless device. This innovative retention method of the frameless intrauterine device ensures a well-tolerated, long-term contraception, allowing for immediate contraception and proper pregnancy spacing for cesarean scar healing, and overcomes the issue of expulsion encountered with conventional intrauterine systems.
The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics
Authors: Youssef Lina , Crovetto Francesca , Simoes Rui Vasco , Miranda Jezid , Paules Cristina , Blasco Miquel , Palomo Marta , García-Calderó Héctor , Tura-Ceide Olga , Dantas Ana Paula , Hernandez-Gea Virginia , Herrero Pol , Canela Núria , Campistol Josep Maria , Garcia-Pagan Joan Carles , Diaz-Ricart Maribel , Gratacos Eduard , Crispi Fatima
Published: January 2022

Introduction: Preeclampsia is a multi-system disorder unique to pregnancy responsible for a great part of maternal and perinatal morbidity and mortality. The precise pathogenesis of this complex disorder is still unrevealed. Methods: We examined the pathophysiological pathways involved in early-onset preeclampsia, a specific subgroup representing its most severe presentation, using LC-MS/MS metabolomic analysis based on multi-level extraction of lipids and small metabolites from maternal blood samples, collected at the time of diagnosis from 14 preeclamptic and six matched healthy pregnancies. Statistical analysis comprised multivariate and univariate approaches with the application of over representation analysis to identify differential pathways. Results: A clear difference between preeclamptic and control pregnancies was observed in principal component analysis. Supervised multivariate analysis using orthogonal partial least square discriminant analysis provided a robust model with goodness of fit (R2X = 0.91, p = 0.002) and predictive ability (Q2Y = 0.72, p < 0.001). Finally, univariate analysis followed by 5% false discovery rate correction indicated 82 metabolites significantly altered, corresponding to six overrepresented pathways: (1) aminoacyl-tRNA biosynthesis; (2) arginine biosynthesis; (3) alanine, aspartate and glutamate metabolism; (4) D-glutamine and D-glutamate metabolism; (5) arginine and proline metabolism; and (6) histidine metabolism. Conclusion: Metabolomic analysis focusing specifically on the early-onset severe form of preeclampsia reveals the interplay between pathophysiological pathways involved in this form. Future studies are required to explore new therapeutic approaches targeting these altered metabolic pathways in early-onset preeclampsia.
Long-Term Follow-Up of Mechanical Circulatory Support in Peripartum Cardiomyopathy (PPCM) Refractory to Medical Management: A Multicenter Study
Authors: Jawad Khalil , Koziarz Alex , Dieterlen Maja-Theresa , Garbade Jens , Etz Christian D. , Saeed Diyar , Langer Elena , Stepan Holger , Scholz Ute , Krause Michael , Brenner Paolo , Schulz Uwe , Borger Michael A. , Eifert Sandra
Published: January 2022

Background: Peripartum cardiomyopathy (PPCM) is a rare, life-threatening form of heart disease, frequently associated with gene alterations and, in some cases, presenting with advanced heart failure. Little is known about ventricular assist device (VAD) implantation in severe PPCM cases. We describe long-term follow-up of PPCM patients who were resistant to medical therapy and received mechanical circulatory support or heart transplant. Methods and results: A total of 13 patients were included with mean follow-up of eight years. Mean age of PPCM onset was 33.7 ± 7.7 years. All patients were initially treated with angiotensin-converting enzyme inhibitors and beta-blockers, and four received bromocriptine. Overall, five patients received VADs (three biventricular, two isolated left ventricular) at median 27 days (range: 3 to 150) following childbirth. Two patients developed drive line infection. Due to the short support time, none of those patients had a stroke or VAD thrombosis. In total, five patients underwent heart transplantation, of which four previously had implanted VADs. Median time to transplantation from PPCM onset was 140 days (range: 43 to 776), and time to transplantation from VAD implantation were 7, 40, 132, and 735 days, respectively. All patients survived until most recent follow up, with the exception of one patient who died following unrelated abdominal surgery two years after PPCM recovery. Conclusions: In patients with severe, life-threatening PPCM refractory to medical management, mechanical circulatory support with or without heart transplantation is a safe therapeutic option.
Insecticidal and Antifungal Activities of Chemically-Characterized Essential Oils from the Leaves of Withania frutescens L.
Authors: Abdelfattah El Moussaoui , Aimad Allali , Bourhia Mohammed , Chebbac Khalid , Salamatullah Ahmad Mohammad , Soufan Walid , Nafidi Hiba-Allah , Aboul-Soud Mourad A. M. , Ouahmane Lahcen , Bari Amina
Published: January 2022

The current study was conducted to investigate antifungal and insecticidal activities of essential oil extracted from the Moroccan Withania frutescens L. (EOW), and their chemical composition was profiled. To achieve this goal, EOW was extracted by the hydro-distillation method and their phytochemical constituents were characterized by gas chromatography-mass spectrometry analyses (GC-MS). Insecticidal activity was evaluated by use of four tests: contact toxicity, inhalation toxicity, and repellent tests. Antifungal activity was evaluated on Fusarium oxysporum f. sp. Ciceris (F. oxysporum) using different concentrations of EOW. GC/MS analysis revealed that EOW was rich in carvacrol (31.87%), thymol (30.08%), and camphor (9.13%). At a 1-µL/L dose, EOW exhibited mortality rates of 23.13 ± 1.07% and 24.41 ± 1.21% against Callosobruchus maculatus (C.maculatus) by inhalation and contact, respectively. Notably, EOW dose of 20 μL/L caused significant mortality rates of 95.1 ± 3.5% and 76.69 ± 1.71% by inhalation and contact, respectively. EOW exhibited an inhibitory effect on mycelial growth against the tested fungi F. oxysporum of 100% and 93.5 ± 1.1% for the 9 and 4.5 mg/mL doses, respectively. The reduced mycelial growth rate for F. oxysporum was recorded to be 0.3 ± 0.1 and 0.6 ± 0.1 mm/h for the EOW doses of 2.25 and 4.5 mg/mL, respectively. The outcome of the present work showed that EOW has a promising antifungal and insecticidal activity, and it can therefore be employed as a natural alternative insecticidal and mycocidal agent to replace the chemically-synthesized ones.
Complete Chloroplast Genome Sequence of Fagus longipetiolata Seemen (Fagaceae): Genome Structure, Adaptive Evolution, and Phylogenetic Relationships
Authors: Liang Daqu , Wang Haoyun , Zhang Jun , Zhao Yuanxiang , Wu Feng
Published: January 2022

Fagus longipetiolata Seemen is a deciduous tree of the Fagus genus in Fagaceae, which is endemic to China. In this study, we successfully sequenced the cp genome of F. longipetiolata, compared the cp genomes of the Fagus genus, and reconstructed the phylogeny of Fagaceae. The results showed that the cp genome of F. longipetiolata was 158,350 bp, including a pair of inverted repeat (IRA and IRB) regions with a length of 25,894 bp each, a large single-copy (LSC) region of 87,671 bp, and a small single-copy (SSC) region of 18,891 bp. The genome encoded 131 unique genes, including 81 protein-coding genes, 37 transfer RNA genes (tRNAs), 8 ribosomal RNA genes (rRNAs), and 5 pseudogenes. In addition, 33 codons and 258 simple sequence repeats (SSRs) were identified. The cp genomes of Fagus were relatively conserved, especially the IR regions, which showed the best conservation, and no inversions or rearrangements were found. The five regions with the largest variations were the rps12, rpl32, ccsA, trnW-CCA, and rps3 genes, which spread over in LSC and SSC. The comparison of gene selection pressure indicated that purifying selection was the main selective pattern maintaining important biological functions in Fagus cp genomes. However, the ndhD, rpoA, and ndhF genes of F. longipetiolata were affected by positive selection. Phylogenetic analysis revealed that F. longipetiolata and F. engleriana formed a close relationship, which partially overlapped in their distribution in China. Our analysis of the cp genome of F. longipetiolata would provide important genetic information for further research into the classification, phylogeny and evolution of Fagus.
Advancing Smart Manufacturing in Europe: Experiences from Two Decades of Research and Innovation Projects
Authors: Grefen Paul , Vanderfeesten Irene , Traganos Kostas , Domagala-Schmidt Zuzanna , van der Vleuten Julia
Published: January 2022

In the past two decades, a large amount of attention has been devoted to the introduction of smart manufacturing concepts and technologies into industrial practice. In Europe, these efforts have been supported by European research and innovation programs, bringing together research and application parties. In this paper, we provide an overview of a series of four content-wise connected projects on the European scale that are aimed at advancing smart manufacturing, with a focus on connecting processes on smart factory shop floors to manufacturing equipment on the one hand and enterprise-level business processes on the other hand. These projects cover several tens of application cases across Europe. We present our experiences in the form of a single, informal longitudinal case study, highlighting both the major advances and the current limitations of developments. To organize these experiences, we place them in the context of the well-known RAMI4.0 reference framework for Industry 4.0 (covering the ISA-95 standard). Then, we analyze the experiences, both the positive ones and those including problems, and draw our learnings from these. In doing so, we do not present novel technological developments in this paper—these are presented in the papers we refer to—but concentrate on the main issues we have observed to guide future developments in research efforts and industrial innovation in the smart industry domain.
Identification of Vehicle System Dynamics from the Aspect of Interaction between the Steering and the Suspension Systems
Authors: Miloradović Danijela , Lukić Jovanka , Glišović Jasna , Miloradović Nenad
Published: January 2022

Steering and suspension systems of a motor vehicle have very important mutual connections that have direct influence on a vehicle’s steerability, stability, comfort and life expectancy. These mechanical and functional couplings cause an intensive interaction between the two mentioned vehicle systems on a geometrical, kinematical and dynamical level. This article presents a study on nonparametric identification of dynamic interaction between the steering and the suspension system of a passenger vehicle. A specific methodology for experimental research in on-road conditions was designed that was in line with the research objectives and the applied measuring system. Experimental data were acquired for a curvilinear drive, with different constant driving speeds and on different roads. A multiple input/multiple output model for identification of the vehicle dynamics system from the aspect of interaction between the steering and the suspension system was developed. The analysis of experimental data was realized with the selection of a corresponding identification model, decoupling of model inputs and conditioned spectral analysis. The results of the conditioned spectral analysis of experimentally obtained data records indicate the level of interaction between the observed input and output parameters of the steering and the suspension systems to be in the frequency range below 30 Hz.