bluuebunny/crossref_metadata_2025 huggingface dataset:
Mid-Term Changes in Soil Properties after Wildfire, Straw Mulching and Salvage Logging in Pinus halepensis Mill. Forests
Authors: Navidi Mehdi , Lucas-Borja Manuel Esteban , Plaza-Álvarez Pedro Antonio , Carra Bruno Gianmarco , Parhizkar Misagh , Zema Demetrio Antonio
Published: October 2022

The hydrological effects of straw mulching and salvage logging have been widely experimented in the Mediterranean forests affected by wildfires. In contrast, knowledge about the impacts of these post-fire management techniques on the physico-chemical properties of burned soils is poor, especially many years after the fire. In particular, no studies have evaluated the soil changes after the combinations of soil mulching and salvage logging after wildfires in Mediterranean forests. To fill this gap, this study explores the effects of straw mulching and salvage logging, applied individually or in combination to a burnt forest of Pinus halepensis Mill. of central-eastern Spain, on the physico-chemical properties of soil six years after a wildfire. Both the post-fire techniques significantly altered the organic matter, phosphorous, and carbonate contents of the burned soils as well as their C/N (carbon/nitrogen) ratio, while the texture and other chemical properties (pH, electrical conductivity, total nitrogen, potassium, cations/anions, and active limestone) of the soils were not significantly affected by these post-fire treatments. Organic matter (OM) and phosphorous (P) contents increased by 57% and 69%, respectively, in mulched soils in comparison to the burned but untreated plots. In logged soils, the OM increased by 27%, while P decreased by 17%. Salvage logging after straw mulching increased OM, albeit less than under the individual soil treatments (+13%), but noticeably reduced P (−39%). The C/N ratio practically underwent the same variation (+15–20%) after the combination of the two treatments. The principal component analysis and the agglomerative hierarchical cluster analysis applied to the soil properties measured in the plots under the individual and combined management show that the effects of salvage logging on soil properties appear to be more impactful compared to straw mulching.
Small-Scale Analysis of Characteristics of the Wildland–Urban Interface Area of Thessaloniki, Northern Greece
Authors: Ganatsas Petros , Oikonomakis Nikolaos , Tsakaldimi Marianthi
Published: October 2022

In the past few years, the continuous expansion of urban development has created mixed forested, agricultural, and urban areas. These areas are called the wildland–urban interface (WUI), and they are characterized by increased human activities and land-use conversion, and they usually contribute to a high risk of wildfire occurrence. In the case of the peri-urban areas of Thessaloniki city, an effort was made to map, classify, and describe this wildland–urban interface, using Sentinel-2 satellite images of the area and very large scale orthophotos (VLSO) for the human settlements. Object-based image analysis (OBIA) was applied to classify landcover, combined with analysis of field data. The results showed that the WUI area in the city of Thessaloniki appears to the north and east of the city and covers an area of 2203.98 ha. The main characteristics affected by the ecological conditions of this area are the building (or human infrastructure) density, type, and the structure of forest vegetation. Human population pressure was found to be greatly differentiated between WUI areas belonging to different municipalities, the most affected was the municipality of Thessaloniki. A set of fire prevention silvicultural treatments are suggested for mitigating the fire danger in the area, accompanied by appropriate human awareness actions and the involvement of the local society. These measures include the reduction in crown bulk density and increase in crown base height through pruning (at least to 1/3 of total tree height), and low thinning, aiming to ensure that tree crowns of mature Pinus brutia trees are not in contact with one another. Both in the young P. brutia forest and the evergreen vegetation areas, thinning, pruning, and vegetation clearing is recommended adjusted according to each ecosystem.
Reproductive Cycle and Sexual Group Maturity of Buccinum osagawai (Neogastropoda: Buccinidae)
Authors: Park Jung Jun , Kim Hyeon Jin , Shin So Ryung , Jin Young Guk , Kim Jae Won , Lee Jung Sick
Published: October 2022

This study was performed to obtain information on the basic reproductive ecology and biological resource management of Buccinum osagawai. Samples were collected from August 2018 to October 2019 with a drum-shaped net at a depth of 150–250 m along the continental shelf in Jumunjin, on the east coast of Korea. The species showed gonochorism and a lack of external sexual dimorphism. The overall sex ratio (F:M) was 1:1.2 (n = 549, F = 250, M = 299); as the size of the individuals increased, the proportion of males tended to decrease. The development pattern of the oocyte was synchronous, in which a dominant group of oocytes was identified within the same ovary. The size of the ripe oocyte was 82.3 (±22.6) × 125.5 (±22.0) μm. The spermatocyte development pattern was group-synchronous, in which multiple stages of germ cell populations were simultaneously identified within the same spermatogenic acinus. The gonad index (GI) for both males and females showed the highest value during June, after which it decreased sharply, and after August it was below 2.0. The main spawning season was from June to July, and the GI and stages of gonadal development did not show a pattern of seasonal changes. The main gonadal active season was from May to July, and both male and female gonadal development and maturation took place over a short period, whereas the recovery period after spawning was longer. At least 60.5% of the group, considering both males and females, showed maturity at 50.1 mm SH or more. Furthermore, the size of 50% group maturity was shown at approximately 50.0 mm SH.
Macro-Habitat Suitability for Threespine Stickleback (Gasterosteus aculeatus L.) Near the Southern Limit of Its Global Distribution: Implications for Species Management and Conservation
Authors: Moreira Andre , Boavida-Portugal Joana , Almeida Pedro R. , Silva Sara , Alexandre Carlos M.
Published: October 2022

The threespine stickleback is a freshwater fish listed as endangered in Portugal, near the southern limit of the species global distribution. However, few measures have been proposed aiming at the conservation of this species in suboptimal environments. From existing databases and specific sampling campaigns, we obtained occurrence data of threespine stickleback for a total of 646 sites. The occurrence data, together with 15 environmental macrohabitat predictors, were used to model the potential distribution of the species using an ensemble of species distribution models. Through the results of our final ensemble, we project that the threespine stickleback occurs predominantly at lower stretches of river systems, where sandy substrate is dominant and flow is higher. Within this region, sticklebacks are also more likely to occur in sites with high levels of rainfall in the driest month, thus avoiding locations with high potential for drying during summer. The species also tends to avoid steep slope areas with high levels of annual precipitation. Based on our results, a map of the species probability of occurrence was generated and river sections were categorized into levels according to their importance for the species’ conservation.
Prokaryotic and Eukaryotic Microbial Community in Kumamoto Oyster (Crassostrea sikamea) Larvae: Response to Antibiotics in Trace Concentration
Authors: Dai Wenfang , Ye Jing , Liu Sheng , Xu Hongqiang , Liu Minhai , Lin Zhihua , Xue Qinggang
Published: October 2022

Antibiotic treatment is regarded as an emergency measure to avoid disease occurrence of aquatic animals during metamorphosis in an aquaculture system, which is very common in hatcheries of bivalve mollusc larvae. However, it is still unclear how and to what extent the antibiotic addition affects the prokaryotic and eukaryotic microbial communities of marine bivalve larvae. We profiled the community compositions and dominant taxonomies of prokaryotic and eukaryotic microbiota of Kumamoto oyster (Crassostrea sikamea) larvae exposed to seawater with antibiotics in trace concentration. A total of 500,664 16S rRNA and 501,933 18S rRNA gene fragments were selected for classification, resulting in 714 prokaryotic Operational Taxonomic Units (OTUs) and 47 eukaryotic OTUs. Antibiotic exposure altered the structure of larval microbiome and increased the prokaryotic but decreased the eukaryotic microbial diversity. Larval microbiota was sensitive to antibiotics, as evidenced by alternation of the dominant bacterial phyla Proteobacteria, Bacteroidetes, Firmicutes, Chlamydiae, and Actinobacteria, and eukaryotic phyla Streptophyta, Cercozoa, Chlorophyta and Haptophyta. Similarly, a significant effect was observed at the family and genus level, especially the increased bacterial Devosiaceae, Microbacteriaceae, Halieaceae, Vibrionaceae families, and Devosia, Stappia and Vibrio genera, and eukaryotic Isochrysidaceae and TAGIRI1-linage family and Tisochrysis genus. These results indicate that antibiotic treatment may induce a shift in the larval microbiome, which may cause an unstable community structure and in turn affect the oyster health.
Effects of Dietary Supplementation with Probiotics and Prebiotics on Growth, Physiological Condition, and Resistance to Pathogens Challenge in Nile Tilapia (Oreochromis niloticus)
Authors: Sîrbu Elena , Dima Maricel Floricel , Tenciu Magdalena , Cretu Mirela , Coadă Marian Tiberiu , Țoțoiu Aurelia , Cristea Victor , Patriche Neculai
Published: October 2022

This study aimed to investigate the effects of the probiotic Betaplus® and prebiotic Technomos® as feed additives for Nile tilapia in terms of growth performance, health profiles, and resistance to infection with Aeromonas hydrophila and Pseudomonas fluorescens. A total of 960 healthy juvenile Nile tilapia (1.5 ± 0.01 g) were randomly divided into four experimental variants with three replicates for each variant. Fish were fed a commercial diet (control group, V0), supplemented with BetaPlus® probiotics–1% × BW (V1), TechnoMos® prebiotics–1% × BW (V2), and with BetaPlus® probiotics and TechnoMos® prebiotics in a ratio of 1:1% × BW (V3). Results on growth performance showed the best values in the probiotic variant, correlated with the health profile and the relative survival percentage after the challenge test with A. hydrophila and P. fluorescens. Similarly, the effects of dietary supplementation with probiotics and prebiotics on physiological conditions also recorded beneficial results in the synbiotic variant, where a high survival percentage was obtained after infection with the two pathogenic bacteria. In conclusion, the results of this study indicate that dietary supplementation with feed additives consisting of mentioned probiotics, prebiotic, and their combination as synbiotics has the potential to promote growth performance, improving tilapia immunity and increasing survival after the challenge test.
Anthropogenic Contaminants Shape the Fitness of the Endangered European Eel: A Machine Learning Approach
Authors: Bourillon Bastien , Feunteun Eric , Acou Anthony , Trancart Thomas , Teichert Nils , Belpaire Claude , Dufour Sylvie , Bustamante Paco , Aarestrup Kim , Walker Alan , Righton David
Published: October 2022

European eel is thought to be a symbol of the effects of global change on aquatic biodiversity. The species has persisted for millions of years and faced drastic environmental fluctuations thanks to its phenotypic plasticity. However, the species has recently declined to historically low levels under synergistic human pressures. Sublethal chemical contamination has been shown to alter reproductive capacity, but the impacts and required actions are not fully addressed by conservation plans. This paper proposes a modelling approach to quantify the effects of sublethal contamination by anthropogenic pollutants on the expression of life history traits and related fitness of the critically endangered European eel. Material and Methods: We sampled female silver eels from eight different catchments across Europe previously shown to be representative of the spectrum of environmental variability and contamination. We measured 11 fitness-related life history traits within four main categories: fecundity, adaptability and plasticity, migratory readiness, and spawning potential. We used machine learning in models to explore the phenotypic reaction (expression of these life history traits) according to geographical parameters, parasite burdens (the introduced nematode Anguillicoloides crassus) and anthropogenic contaminants (persistent organic pollutants (POPs) in muscular tissue and trace elements (TEs) in gonads, livers and muscles). Finally, we simulated, the effects of two management scenarios—contamination reduction and contamination increase—on the fecundity and recruitment. Results: Contamination in our sampling was shown to have a stronger control on life history traits than do geographic and environmental factors that are currently described in the literature. We modelled different contamination scenarios to assess the benefit of mitigation: these scenarios suggest that reducing pollutants concentrations to the lowest values that occurred in our sampling design would double the fecundity of eels compared to the current situation. Discussion: Remediation of contamination could represent a viable management option for increasing the resilience of eel populations, with much more effects than solely reducing fishing mortality. More broadly, our work provides an innovative way for quantitative assessment of the reaction norms of species’ biological traits and related fecundity to contamination by organic and inorganic pollutions thus opening new management and conservation pathways to revert the erosion of biodiversity.
Exploring the Response of the Japanese Sardine (Sardinops melanostictus) Stock-Recruitment Relationship to Environmental Changes under Different Structural Models
Authors: Bai Xuan , Gao Li , Choi Sangduk
Published: October 2022

Japanese sardines (Sardinops melanostictus) are environmentally sensitive pelagic fish. We investigated the effects of environmental factors on the stock-recruitment (S-R) relationship of S. melanostictus in the Pacific Ocean from 1984 to 2018. We modeled and analyzed the recruitment of S. melanostictus using the Ricker model, the Ricker environment extension model (Ricker-E), and the generalized additive model (GAM). Different numbers of environmental factors were added to the models for fitting, and the Akaike information criterion (AIC) was used to select the optimal model. The results showed that the nonlinear GAM provided the best fit. The results of the GAM single factor and multifactor analysis showed that environmental factors were significantly correlated with recruitment in S. melanostictus (p < 0.05). The best fitting model was the GAM, with an AIC of 57.9 and a cumulative explanation rate of 95.7%. Sea surface temperature was the most dominant environmental factor and had a negative impact on R/S. Considering both spawning stock biomass (S) and the environmental factors in the S-R relationship is important for studying the mechanisms of complementary changes and population changes in S. melanostictus under the influence of the marine environment.
Physics of Dynamic Contact Line: Hydrodynamics Theory versus Molecular Kinetic Theory
Authors: Mohammad Karim Alireza , Suszynski Wieslaw J.
Published: October 2022

The dynamic contact line plays a key role in various fields of interfacial physics, including bioprinting, nano-scale printing, three-dimensional printing, biomaterials, tissue engineering, smart materials, flexible printed electronics, biomedicine, and healthcare. However, there is still a lack of thorough physical understanding of its real behavior in numerous complex problems in nature and technology. The dynamic contact line exhibits a complex conformation in real-life fluid dynamics problems. Therefore, this review presents two main long-standing models that describe the physics of the dynamic contact line: hydrodynamics theory and molecular kinetics theory. Next, the role of the dynamic contact line in current advanced technologies is discussed. Finally, this review discusses future research directions to enhance the power of current physical models of the dynamic contact line.
Research Progress of Air Lubrication Drag Reduction Technology for Ships
Authors: An Hai , Pan Haozhe , Yang Po
Published: October 2022

Air lubrication is a promising drag reduction technology for ships because it is considered to reduce the skin-friction resistance of ships by changing the energy of turbulent boundary layers. Air lubrication drag reduction can be classified into: microbubble drag reduction (injection of microbubbles along the hull), air film drag reduction (using a larger film of air to cover the ship bottom), and air cavity drag reduction (recesses underneath the hull are filled with air). In this paper, the research progress of the air lubrication drag reduction technology is reviewed from experimental and numerical aspects. For these three drag reduction methods, based on the aspect of experimental research, the main research focus is the analysis and evaluation of the influencing factors such as the gas injection form and drag reduction rate; in terms of theoretical research, the accuracy of the simulation calculation depends on the selection of the theoretical calculation model and the analysis of the drag reduction mechanism. The paper introduces, in detail, the typical experimental phenomena and the theoretical results of a numerical study of three types of drag reduction methods, revealing the essence of air lubrication technology to achieve drag reduction by changing the physical properties of the turbulent boundary layer.
The Single Particle Motion of Non-Spherical Particles in Low Reynolds Number Flow
Authors: Mendez Yuri
Published: October 2022

This research presents a mathematical framework that places the physics and the dynamics of viscosity within a physical environment that captures the effect of the shape and overall weight of non-spherical particles to calculate their settling velocity. It then takes insights derived from the framework to model analytical constructs to solve the motion of a single particle that settles in a fluid that moves horizontally as a whole. These analytical constructs are then shown to be applicable to spherical and non-spherical particles.
Impact of the Soundproofing in the Cavity of the Synthetic Jet Actuator on the Generated Noise
Authors: Smyk Emil , Markowicz Marek
Published: October 2022

The synthetic jet actuator (SJA) generated high noise which limits the area of its application. In this paper, the five actuators with different types of soundproofing in the cavity were tested and compared to the classic actuator. The resistance and the sound pressure level (SPL) were measured for real power P=1, 2, 4 W, and frequency in a range of 20–150 Hz. The resonant frequency of actuators was designed. Only one type of soundproofing had a significant impact on the resonant frequency. The use of soundproofing in the actuator cavity increased or did not affect the generated noise at a frequency below 120 Hz and only the mineral wool significantly decreased the noise at a frequency above 120 Hz– even 7 dBA. The direction for further investigations was set.
Analysis of Food Delivery Using Big Data: Comparative Study before and after COVID-19
Authors: Jang Jina , Lee Eunjung , Jung Hyosun
Published: October 2022

This study examined consumers’ change in perception related to food delivery using big data before and after the COVID-19 crisis. This study identified words closely associated with the keyword “food delivery” based on big data from social media and investigated consumers’ perceptions of and needs for food delivery and related issues before and after COVID-19. Results were derived through analysis methods such as text mining analysis, Concor analysis, and sentiment analysis. The research findings can be summarized as follows: In 2019, frequently appearing dining-related words were “dining-out,” “delivery,” “famous restaurant,” “delivery food,” “foundation,” “dish,” “family order,” and “delicious.” In 2021, these words were “delivery,” “delivery food,” “famous restaurant,” “foundation,” “COVID-19,” “dish,” “order,” “application,” and “family.” The analysis results for the food delivery sentimental network based on 2019 data revealed discourses revolving around delicious, delivery food, lunch box, and Korean food. For the 2021 data, discourses revolved around delivery food, recommend, and delicious. The emotional analysis, which extracted positive and negative words from the “food delivery” search word data, demonstrated that the number of positive keywords decreased by 2.85%, while negative keywords increased at the same rate. In addition, compared to the pre-COVID-19 pandemic era, a weakening trend in positive emotions and an increasing trend in negative emotions were detected after the outbreak of the COVID-19 pandemic; sub-emotions under the positive category (e.g., good feelings, joy, interest) decreased in 2021 compared to 2019, whereas sub-emotions under the negative category (e.g., sadness, fear, pain) increased.
A Comparative Study of Ten Pear (Pyrus communis L.) Cultivars in Relation to the Content of Sugars, Organic Acids, and Polyphenol Compounds
Authors: Akagić Asima , Oras Amila , Gaši Fuad , Meland Mekjell , Drkenda Pakeza , Memić Senad , Spaho Nermina , Žuljević Sanja Oručević , Jerković Igor , Musić Osman , Hudina Metka
Published: October 2022

Traditional pear cultivars are increasingly in demand by consumers because of their excellent taste, the possibility of use in sustainable food production systems, convenience as raw materials for obtaining products of high nutritional quality, and perceived health benefits. In this study, individual sugars, organic acids, and polyphenols in the fruits of nine traditional and one commercial pear cultivar during two growing seasons were determined by HPLC. A significant influence of cultivars, growing years, and their interaction on the content of analyzed primary and secondary metabolites was determined. The commercial pear cultivar ‘Président Drouard’ and traditional cultivars ‘Dolokrahan’, ‘Budaljača’, and ‘Krakača’ had a lower content of all analyzed sugars. Overall, traditional pear cultivars had higher total polyphenols in the peel and pulp than ‘Président Drouard’, with the exception ‘Takiša’ and ‘Ahmetova’. High polyphenol content detected in ‘Budaljača’, ‘Dolokrahan’, and ‘Krakača’ shows the utilization value of traditional pear germplasm. The obtained data can serve as practical supporting data for the use of traditional pears in the neutraceutical, pharmaceutical, and food industries.
A New HPLC-MS/MS Method for the Simultaneous Determination of Quercetin and Its Derivatives in Green Coffee Beans
Authors: Mustafa Ahmed M. , Abouelenein Doaa , Angeloni Simone , Maggi Filippo , Navarini Luciano , Sagratini Gianni , Santanatoglia Agnese , Torregiani Elisabetta , Vittori Sauro , Caprioli Giovanni
Published: October 2022

Green coffee (Coffee arabica and Coffee robusta) is one of the most commonly traded goods globally. Their beans are enriched with polyphenols and numerous health benefits are associated with their consumption. The main aim of this work was to develop a new and fast analytical HPLC-MS/MS method to simultaneously determine six flavonoid polyphenolic compounds (quercetin, rutin, isorhamnetin, quercetin-3-glucouronide, hyperoside, and quercitrin) in 22 green coffee samples from six different geographical origins (Ethiopia, Brazil, Guatemala, Nicaragua, India and Colombia). In addition, by adjusting pH, temperature, solvent type, and extraction duration, several extraction methods such as acidic and alkaline hydrolysis, and extraction without hydrolysis were evaluated. The optimal extraction procedure in terms of recovery percentages (78.67–94.09%)was acidic hydrolysis at pH 2, extraction temperature of 60 °C, extraction solvent of 70% ethanol, and extraction duration of 1.5 h. Hyperoside (878–75 μg/kg) was the most abundant compound followed by quercitrin (408–38 μg/kg), quercetin (300–36 μg/kg), rutin (238–21 μg/kg), and quercetin-3-glucouronide (225–7 μg/kg), while isorhamnetin (34–3 μg/kg) showed the lowest amount. Overall, green coffee beans are rich in flavonoid polyphenolic compounds and could be used as part of a healthy diet.
Experimental Investigation of a New Modular Crusher Machine Developed for Olive Oil Extraction Plants
Authors: Tamborrino Antonia , Perone Claudio , Veneziani Gianluca , Berardi Antonio , Romaniello Roberto , Servili Maurizio , Leone Alessandro
Published: October 2022

The crushing system is crucial in the virgin olive oil (VOO) mechanical extraction process. The use of different crusher machines can highly influence the quality of the final product, mainly due to the phenolic and volatile content responsible for VOO sensory and health properties. An experimental investigation was conducted to evaluate the effect of the geometric features of a new model of crusher machine for olives. The crusher machine consists of interchangeable rotors: a rotor with hammers and a rotor with knives. The evaluation was carried out with the same fixed grid in stainless steel with 6 mm diameter circular holes. An evaluation was carried out on the impact of the crusher tools on the pit particle size and on the distribution of energy and temperature. The performance of the plant was also assessed in terms of process efficiency and olive oil quality. The results showed that the specific energy released by the tool per unit of product, calculated through both energy conservation and comminution theory, is about 25–27% higher in the case of hammers. Since the impact energy is mainly dissipated in the product as heat, the temperature reached during milling operations with the hammer crusher was also higher by the same percentage with respect to the knife crusher. This has important consequences on the quality of the product: the new knife rotor used in the crushing phase produced an improvement in VOO quality, relating mainly to sensory attributes and the health-enhancing properties of the final product. The ability of the crusher to break cell walls and vacuoles, thus releasing the oil contained therein, is comparable for the two different rotors.
Glucosinolates and Polyphenols of Colored Cauliflower as Chemical Discriminants Based on Cooking Procedures
Authors: Nartea Ancuta , Fanesi Benedetta , Giardinieri Alessandra , Campmajó Guillem , Lucci Paolo , Saurina Javier , Pacetti Deborah , Fiorini Dennis , Frega Natale Giuseppe , Núñez Oscar
Published: October 2022

The impact of mild oven treatments (steaming or sous-vide) and boiling for 10 min, 25 min, or 40 min on health-promoting phytochemicals in orange and violet cauliflower (Brassica oleracea L. var. botrytis) was investigated. For this purpose, targeted ultra-high performance liquid chromatography–high-resolution mass spectrometry analysis of phenolics and glycosylates, combined with chemometrics, was employed. Regardless of cooking time, clear differentiation of cooked samples obtained using different procedures was achieved, thus demonstrating the distinct impact of cooking approaches on sample phytochemical profile (both, compound distribution and content). The main responsible components for the observed discrimination were derivatives of hydroxycinnamic acid and kaempferol, organic acids, indolic, and aromatic glucosinolates, with glucosativin that was found, for the first time, as a discriminant chemical descriptor in colored cauliflower submitted to steaming and sous-vide. The obtained findings also highlighted a strict relationship between the impact of the cooking technique used and the type of cauliflower. The boiling process significantly affected the phytochemicals in violet cauliflower whereas orange cauliflower boiled samples were grouped between raw and either steamed or sous-vide-cooked samples. Finally, the results confirm that the proposed methodology is capable of discriminating cauliflower samples based on their phytochemical profiles and identifying the cooking procedure able to preserve bioactive constituents.
Lycopene-Loaded Bilosomes Ameliorate High-Fat Diet-Induced Chronic Nephritis in Mice through the TLR4/MyD88 Inflammatory Pathway
Authors: Liu Chang , Liu Yu , Wang Ciwan , Guo Yahui , Cheng Yuliang , Qian He , Zhao Yong
Published: October 2022

Chronic kidney disease caused by a high-fat diet (HFD)-induced metabolic syndrome has received widespread attention. Lycopene has a wide range of biological activities and can improve a variety of chronic diseases through anti-inflammatory effects. In this study, HFD-fed mice were used as a metabolic syndrome model to evaluate the protective effect of lycopene in a sustained-release vehicle (bilosomes) in the small intestine against renal injury and to determine whether the TLR4/MyD88 pathway and related metabolic pathways are involved in this process. The results showed that lycopene bilosomes alleviated HFD-induced kidney damage, as evidenced by lower serum urea nitrogen, creatinine, and uric acid levels. Histopathology studies showed that lycopene bilosomes attenuated HFD-induced tubular cell and glomerular injury. In addition, Elisa, RT-PCR, and Western blotting results showed that lycopene bilosomes also reduced the expression of inflammatory factors such as TLR4, MyD88, NF-kB, TNF-a, and IL-6 in mouse kidneys. The mechanism was to attenuate renal inflammatory response by inhibiting the TLR4/MyD88 inflammatory pathway. These findings suggested that lycopene can alleviate nephritis and metabolic disorders caused by HFD, inhibiting the TLR4/MyD88 inflammatory pathway and its downstream pro-inflammatory cytokines and further regulating the vitamin K metabolism, beta-alanine metabolism, and glutathione metabolism pathways to relieve chronic nephritis.
Role and Importance of Functional Food Packaging in Specialized Products for Vulnerable Populations: Implications for Innovation and Policy Development for Sustainability
Authors: Pascall Melvin A. , DeAngelo Kris , Richards Julie , Arensberg Mary Beth
Published: October 2022

Specialized products can be needed to help meet the nutrition requirements of vulnerable populations, including infants and young children, those who are ill, and older adults. Laws and regulations delineate distinct categories for such products including medical foods or formulated liquid diets, foods for special dietary use (FSDUs), infant formulas, and natural health products (NHPs). Yet, the literature is limited regarding the role and importance of functional and sustainable packaging for specialized products. This perspective review describes these unique product categories and the role of packaging as well as regulatory considerations. Furthermore, reviewed are how waste reduction strategies and emerging legislative/regulatory policies in the United States and Canada may not adequately address the functional packaging requirements for specialized products. The paper concludes by offering perspectives for emerging innovations and policy development for sustainability.
Sustainable Consumption: Will They Buy It Again? Factors Influencing the Intention to Repurchase Organic Food Grain
Authors: Thanki Heena , Shah Sweety , Oza Ankit , Vizureanu Petrica , Burduhos-Nergis Dumitru Doru
Published: October 2022

Global consumption trends point to rising demand for organic food as people become more health-conscious. The factors that people consider while making initial organic purchases have been discussed at length. However, the published research is scant about the factors that affect consumers’ propensity to repurchase organic goods. The present research fills this gap by focusing on what influences consumers’ decisions to repurchase organic grain. The Stimulus-Organism-Response (S-O-R) theory and the Theory of Planned Behaviour are the theoretical foundations of the present investigation. The consumer’s attitude toward organic grains and their desire to repurchase organic grains are influenced by health consciousness and previous experience. The repurchase intent was determined to be controlled by the buyer’s willingness to pay and their level of trust in the organic grain. This cross-sectional study collected the necessary data from five chosen urban centres in India. Smart PLS 3.2.9 was used to analyse the gathered data from 463 respondents. According to the findings, health consciousness and past experience favourably influence attitudes and repurchase intent. The trust that consumers have built up in organic grain as a result of past experiences is what drives their desire to make more purchases. Willingness to pay significantly controls and impacts the inclination to repurchase. The association between health consciousness and repurchase intention is partially mediated by attitude, as is the relationship between past experience and repurchase intention. The relationship between health consciousness and the desire to repurchase is partially mediated by the willingness to pay.
Method Validation and Assessment of Hazardous Substances and Quality Control Characteristics in Traditional Fruit Wines
Authors: Baek Chae-Wan , Chang Hyeon-Jun , Lee Jeung-Hee
Published: October 2022

The presence of potentially hazardous substances in fruit wines poses a threat for human health. However, the management standards and specifications of hazardous substances contained within various types of fruit wines are currently insufficient. The aim of this study was to analyze hazardous substances (cyanide, acetaldehyde, and ethyl carbamate) and quality control characteristics (pH, titratable acidity, sulfur dioxide, and diacetyl) in seven different types of fruit wines. The pH levels and titratable acidity varied between fruit wine types. In all fruit wines, sulfur dioxide (SO2) was within acceptable ranges as per the Korean standard. Acetaldehyde content also varied between fruit wine types as well as based on the analytical method (titration or enzymatic analysis) employed. Cyanide was in the range of 0.02–0.35 mg/L. Diacetyl contents were in the range of 0.66–2.95 mg/L (p > 0.05). The contents of ethyl carbamate varied considerably, within the range of 5.22–259.69 μg/kg (p < 0.05). The analytical methods of diacetyl and ethyl carbamate were validated for specificity, linearity, sensitivity, accuracy, and precision. Therefore, the content of hazardous substances and quality control characteristics should be closely monitored and controlled to improve safety and quality of the traditional fruit wines.
Effect of Bioprocessing on Techno-Functional Properties of Climate-Resilient African Crops, Sorghum and Cowpea
Authors: Nikinmaa Markus , Renzetti Stefano , Juvonen Riikka , Rosa-Sibakov Natalia , Noort Martijn , Nordlund Emilia
Published: October 2022

Sorghum and cowpea are very compatible for intercropping in hot and dry environments, and they also have complementary nutritional compositions. Thus, the crops have the potential to improve food security in regions threatened by climate change. The aim of this study was to investigate different enzymes (carbohydrate-degrading, proteases and phytases) and lactic acid bacteria (LAB) fermentation to improve the techno-functional properties of sorghum and cowpea flours. Results show that sorghum carbohydrates were very resistant to hydrolysis induced by bioprocessing treatments. Most of the protease treatments resulted in low or moderate protein solubilization (from ca. 6.5% to 10%) in sorghum, while the pH adjustment to 8 followed by alkaline protease increased solubility to 40%. With cowpea, protease treatment combined with carbohydrate-degrading enzymes increased the solubility of proteins from 37% up to 61%. With regard to the techno-functional properties, LAB and amylase treatment decreased the sorghum peak paste viscosities (from 504 to 370 and 325 cPa, respectively), while LAB and chemical acidification increased cowpea viscosity (from 282 to 366 and 468 cPa, respectively). When the bioprocessed sorghum and cowpea were tested in breadmaking, only moderate effects were observed, suggesting that the modifications by enzymes and fermentation were not strong enough to improve breadmaking.
Study of Lactic Acid Bacteria Biodiversity in Fermented Cobrançosa Table Olives to Determine Their Probiotic Potential
Authors: Coimbra-Gomes Joana , Reis Patrícia J. M. , Tavares Tânia G. , Malcata Francisco Xavier , Macedo Angela C.
Published: October 2022

Current market trends point at increasing demand for functional foods, namely those carrying probiotics. In the case of table olives, presence of probiotics would convey a competitive advantage to Mediterranean-based diets, already established for their cultural heritage and gastronomic character. This work assessed the safety and resistance to gastrointestinal digestion of 19 native LAB strains from Cobrançosa table olives. Strains were identified via molecular sequencing (4 fingerprints/10 strains for Lactiplantibacillus pentosus, and 2 fingerprints/9 strains for L. paraplantarum), and exposed to simulated gastrointestinal fluids, as per the INFOGEST in vitro protocol with modifications. None of those strains proved dangerous for human consumption. Survivability to the gastrointestinal resistance test ranged from 29% to 70%, with strain-dependent variability. L. paraplantarum i18, i27, and i102, and L. pentosus i10 and i11 exhibited statistically lower survival rates (29–35%) than probiotic the Greek table olive reference strain L. pentosus B281 (53%). Among the other strains, L. paraplantarum i101 and L. pentosus i53 and i106 showed the highest survival rates but were not significantly different from the strain of Lacticaseibacillus casei isolated from commercial probiotic yoghurt (65–70%). In vitro results proved that strains retrieved from fermenting cultivar Cobrançosa possess the potential to be claimed as probiotics—thus deserving further attention toward the development of a specific starter culture.
Development of Antimicrobial Cellulose Nanofiber-Based Films Activated with Nisin for Food Packaging Applications
Authors: Maresca Diamante , Mauriello Gianluigi
Published: October 2022

The cellulose nanofiber (CNF) is characterized by the nano-sized (fibers with a diameter between 5 and 20 nm and a length between 2 and 10 μm), flexible and cross-linked structure that confer enhanced mechanical and gas barrier properties to cellulosic fiber-based packaging materials. The purpose of this work was to develop an antimicrobial packaging film by direct mixing nisin with CNF, followed by coating it onto polyethylene (PE), polypropylene (PP), and polylactic acid (PLA) films. The antimicrobial effectiveness of CNF-Nis+PE, CNF-Nis+PP, and CNF-Nis+PLA was investigated both in vitro end in ex vivo tests. In the latter case, challenge test experiments were carried out to investigate the antimicrobial activity of the coupled films of CNF-Nisin+PLA to inhibit the growth of Listeria innocua 1770 during the storage of a meat product. The films were active against the indicator microorganisms Brochothrix thermosphacta and Listeria innocua in in vitro test. Moreover, a reduction in the Listeria population of about 1.3 log cycles was observed immediately after the contact (T0) of the active films with hamburgers. Moreover, when the hamburgers were stored in active films, a further reduction of the Listeria population of about 1.4 log cycles was registered after 2 days of storage. After this time, even though an increase in Listeria load was observed, the trend of the Listeria population in hamburgers packed with active films was maintained significantly lower than the meat samples packed with control films during the whole storage period.
Factors Influencing the Consumption of Seaweed amongst Young Adults
Authors: Young Mikaela , Paul Nicholas , Birch Dawn , Swanepoel Libby
Published: October 2022

Seaweed has been traditionally consumed in Asian and Pacific cultures, yet aside from sushi, is still not commonly eaten in Western societies. Edible seaweeds offer distinct nutritional benefits to terrestrial crops, particularly with respect to mineral and fibre content. Understanding the motivations that drive young Australians to eat seaweed is necessary for food product development and consumer marketing strategies, as well as informing future sustainable production through seaweed aquaculture and wild-harvest practices. An observational cross-sectional online survey with n = 1403 young (19–30 years) Australian seaweed consumers was conducted. The 19-item survey included closed-ended, open-ended, and Likert scale responses. Most respondents were female (89.0%), with tertiary level education or above (57.7%). Seaweed was eaten mostly as a snack (87.7%) and in home-prepared meals (30.7%). The key advantages to consumption were flavour (89.1%), nutrient content (49.1%), and health benefits (44.6%), whilst the key barriers were poor accessibility (59.5%), unaffordable pricing (46.5%), and undesirable packaging (19.0%). The consumers reported wanting more promotion to improve their knowledge about seaweed, in addition to environmentally sustainable packaging and sourcing. Pathways to overcome barriers and encourage greater seaweed consumption are discussed. Most critically, improving the promotion and environmental sustainability of seaweed products will improve intake amongst current and future consumers.
Life Cycle Assessment (LCA) of Substrate Mixes Containing Port Sediments for Sustainable ‘Verna’ Lemon Production
Authors: Hernández Francisca , Martínez-Nicolás Juan , Melgarejo Pablo , Núñez-Gómez Dámaris , Lidón Vicente , Martínez-Font Rafael , Legua Pilar
Published: October 2022

The increase in maritime trade and its global economic importance have forced port management actors to carry out the periodic dredging of their sediments to maintain an adequate depth for the passage of large ships to maintain their operation and competitiveness. During the dredging process, large volumes of port sediment are generated. Dredged port sediment is currently considered a waste material and its disposal is regulated. Finding ways to safely reuse port sediments is necessary for sustainable development. In this study, a life cycle assessment (LCA) methodology was applied to identify the environmental impact of port sediments when used as a culture medium for lemon trees. A total of 90 lemon trees (Citrus limon L. Burm var ‘Verna’) were used in the trial. The trees were grown under controlled conditions using three substrates, with different portions of peat and port sediment (25%, 50%, and 75%) to identify the real impacts of the culture media on the growth process. The LCA was calculated and analyzed according to the ISO 14040:2006 standard, using the SimaPro v. 9.3 software (PRé Sustainability B.V, Amersfoort, The Netherlands). The functional unit defined for the three-culture media was 1 kg of lemons. The LCA results showed a significant increase in the environmental impact of lemon cultivation proportional to port sediment content (75%), due to the decrease in fruit production caused by the sediment. However, the least impact was identified for the culture medium at 50% peat and 50% port sediment. The greatest impacts were more related to crop management rather than the port sediment content. The results showed that the use of the port sediment, mixed with other substrates as an agricultural medium amendment, is a viable option for lemon growers.
Isotope Geochemistry for Seafood Traceability and Authentication: The Northern Adriatic Manila Clams Case Study
Authors: Brombin Valentina , Natali Claudio , Frijia Gianluca , Schmitt Katharina , Casalini Martina , Bianchini Gianluca
Published: October 2022

In Italy, the production of manila clams (Ruditapes philippinarum, Adams and Reeve, 1850) is mainly localized in northern Adriatic lagoons in the Po River delta, where shellfish farming provides important socio-economic revenue. However, in our globalized world, the seafood market is threated by fraudulent activities, in which agri-food products whose provenance is not certified are sold, posing a risk to consumer health. Multi-isotope ratio analysis is commonly used to trace the provenance of goods produced in different countries with different climatic and environmental conditions. Here, we investigated the reliability of this approach in terms of tracing the exact provenance of manila clams harvested in three Adriatic northern lagoons that are close to each other. We also verified the origin of samples bought at a local supermarket with a certificate of provenance. We carried out elemental analyses of carbon (C), nitrogen (N), and sulfur (S) and the respective isotopic ratios (13C/12C; 15N/14N; 34S/32S) on manila clam tissues, plus isotopic analyses of carbon (13C/12C), oxygen (18O/16O), and strontium (87Sr/86Sr) on manila clam shells. Each isotopic parameter can be used to identify the marine and continental contributions of water and/or nutrient supplies occurring in the lagoons. Therefore, the combination of isotopic parameters in a linear discriminant analysis (LDA) allowed for the identification of the lagoons in which the manila clams were produced.
Red Beetroot Fermentation with Different Microbial Consortia to Develop Foods with Improved Aromatic Features
Authors: Casciano Flavia , Mayr Hannah , Nissen Lorenzo , Putti Andreas , Zoli Federica , Gianotti Andrea , Conterno Lorenza
Published: October 2022

The European culinary culture relies on a wide range of fermented products of plant origin, produced mostly through spontaneous fermentation. Unfortunately, this kind of fermentations is difficult to standardize. Therefore, the use of commercial starter cultures is becoming common to achieve more stable, reproducible, and predictable results. Among plant-based fermentation processes, that of the red beet (Beta vulgaris L. var. conditiva) is scarcely described in the scientific literature. In this work, we compared different types of fermentation methods of beetroot and evaluated the processes’ micro-biological, physico-chemical, structural, and volatilome features. A multi-variate analysis was used to match the production of specific VOCs to each starter and to define the correlations between the process variables and volatilome. Overall, the results showed a successful lactic acid fermentation. The analysis of the volatilome clearly discriminated the metabolic profiles of the different fermentations. Among them, the sample fermented with the mixture was the one with the most complex and diversified volatilome. Furthermore, samples did not appear softened after fermentation. Although this work had its weaknesses, such as the limited number of samples and variety, it may pave the way for the standardization of artisanal fermentation procedures of red beetroot in order to improve the quality and safety of the derived food products.