bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of microbial diversity and community structure of rhizosphere soil of Cistanche salsa from different host plants
Authors: Liu AiLing , Li YuXia , Wang QiQi , Zhang XinRui , Xiong Jie , Li Yang , Sun YanFei , Lei YongHui
Published: May 2022

Host plants influence Cistanche germination through root secretions, and plant rhizosphere bacteria influence host growth. Therefore, investigating the distribution of rhizosphere microorganisms in these host plants is crucial. Currently, good literature comparing the rhizosphere soil bacteria and fungi of different host plants of Cistanche salsa in the same habitat is lacking. High-throughput sequencing was used here to reveal the similarities and differences in the structural composition of the soil microbial community of C. salsa from six host plants (i.e., Halocnemum, Atriplex, Kalidium, Tamarix, Anabasis, and Ceratoides). We discovered that Ceratoides-parasitizing C. salsa had the highest diversity of rhizosphere bacterial communities, and Anabasis-parasitizing C. salsa had the highest diversity of rhizosphere fungal communities. norank_f__norank_o__Actinomarinales and Aspergillus were the most dominant bacterial and fungal genera, respectively. However, differences were noted in the contents of individual samples, which were attributable to the different host plants. RDA results also revealed that AP, HCO3−, pH, Ca2+, SO42−, and K+ had a highly significant impact on the bacterial community structure (P<0.01), while pH and SO42− had a significant impact on the fungal community structure (P<0.05). Moreover, bacteria are more vulnerable than fungi to soil physical and chemical indicators. Conclusively, differences were noted in the structure of rhizosphere bacterial and fungal communities of C. salsa parasitizing different plants in the same habit. A close correlation was observed between C. salsa and their host plants, soil microorganisms, and soil physical and chemical indicators. Our study findings can provide novel ideas for host plant selection and C. salsa cultivation.
Factors associated with prescribing physiotherapy for adult patients consulting for musculoskeletal disorders in primary care; an ancillary study to ECOGEN
Authors: Peurois Matthieu , Bertin Melanie , Fouquet Natacha , Adjeroud Najia , Roquelaure Yves , Ramond-Roquin Aline
Published: May 2022

Background: Musculoskeletal disorders (MSD) are multifactorial requiring multidisciplinary treatment including physiotherapy. General practitioners (GP) have a central role in managing MSDs and mostly solicit physiotherapists accounting for 76.1% of physiotherapy prescriptions in France. Patient, physician, and contextual factors, including healthcare accessibility, can influence physiotherapy prescription rates.Objective: To identify patient, physician, and contextual factors associated with physiotherapy prescription in adult patients with MSDs in general practice. Methods: This study is based on the 2011/2012 French cross-sectional ECOGEN study. Analyses included working-age patients consulting their GP for any MSD. Physiotherapy prescription was assessed initially, then adjusted multilevel logistic model analysis of patient, physician, geographical area-related factors associated with these prescriptions was performed. Results: Among the 2305 patients included, 456 (19.8%) were prescribed physiotherapy. Following multilevel multivariate analyses, physiotherapy was prescribed more frequently for female patients (OR 1.28; 95% CI [1.03, 1.59]) with spinal (OR 1.47; 95% CI [1.18, 1.83]) and upper limb disorders (OR 1.66; 95% CI [1.20, 2.29]), and less frequently for patients >50 years (OR 0.69; 95% CI [0.52, 0.91]), living in deprived geographical areas (OR 0.60; 95% CI [0.40, 0.90]). GPs prescribed physiotherapy less frequently if they were > 50 years (OR 0.50; 95% CI [0.39, 0.63]), had a high number of annual consultations, or were practicing in semi-urban area in a multidisciplinary team.Conclusion: This multilevel analysis identifies factors associated with physiotherapy prescription for patients with MSDs, including living in deprived geographical areas. This constitutes an original contribution towards addressing healthcare disparities.
Optimization of surface grinding parameters used in improved surface Integrity
Authors: Salem M. M. , Mohamed S. S. , Ibrahim A. A.
Published: May 2022

Surface grinding is one of the most extensively used procedures in industry for finishing materials. Different machining parameters have a great impact on a product's surface quality. While processing temperature increases and heat energy is generated, that may cause a few changes in the tool or workpiece in chemical and physical properties. This will have an effect on the final product surface. Furthermore, numerous aspects of the machine, such as vibration, degree of freedom, and precise size, have an impact on the quality of the surface finish. The machining parameters, on the other hand, have a major role in determining the surface finish.The functional behavior of machined components can be enhanced by the grinding process, so proper selection of grinding wheel specifications and grinding parameters can result in improving the surface integrity.Because of this, understanding the surface integrity imparted by grinding is very important.This paper will display the results of an experimental study that were conducted to comprehensively investigate the effect of surface grinding input machining parameters on the formed surface quality. The experiments are running by changing the input machine parameters which are the feed rate, cutting speed, cooling method and depth of cut. The surface roughness and the cutting forces are the performance to be measured and assessed.It is concluded that the surface roughness (Ra) decreases with the increase in cutting speed and the coolant flow rate. But, as the feed rate and or the depth of cut increase the surface roughness (Ra) will also increase. The results of this investigation can be used to determine the optimal process parameters in order to obtain the required roughness quality of the produced surface in grinding of (ASTM A36) mild carbon steel.
The antioxidant and hepatoprotective potential of Solanum nigrum against oxidative stress
Authors: Alam Amna , Sahar Amna , Sameen Aysha , Faisal Muhammad Naeem
Published: May 2022

The present study aimed to evaluate the phytochemical screening and in vivo antioxidant potential of Solanum nigrum against phenylhydrazine induced liver injury. The Solanum nigrum extract has significant in vitro antioxidant activity. Moreover, HPLC analysis of ethanolic Solanum nigrum extract showed revealed the presence of phenolic compounds. The Sprague Dawley rats were divided into four groups for in vivo analysis: G1 (negative control group), G2 (positive control group), G3 (rats receiving standard drug), and G4 (rats receiving Solanum nigrum extract i.e.1.0g/kg b.w.). The Solanum nigrum also reduced the level of liver enzyme, and bilirubin. An opposite trend was seen in the case of albumin, catalase, and superoxide dismutase. Histology slides also showed the normal cell structure of hepatocytes. Gene expression analysis demonstrates the upregulation of antioxidant and anti-apoptosis genes. Conclusively, Solanum nigrum has the ability to alleviate liver toxicity in an animal model by reducing oxidative stress and downregulating the apoptosis genes.
Halide Perovskite Glues Activate Two-dimensional Covalent Organic Framework Crystallites for Selective Gas Sensing
Authors: Ye Wen , Zhao Liangdan , Lin Hongzhen , Ding Lifeng , Cao Qiang , Chen Ze-Kun , Wang Jia , Sun Qi-Meng , He Jing-Hui , Lu Jian-Mei
Published: May 2022

Two-dimensional covalent organic frameworks (2D COFs) are promising for gas sensing owing to the large surface area, size-tunable pores, abundant active sites, and their semiconducting nature. However, 2D COFs are usually produced in the form of insoluble micro crystallites. Their poor contacts between grain boundaries severely suppress the conductivity, which are too low for chemresistive gas sensing. Here, we demonstrate that halide perovskite nanocrystals can be employed as electric glues to bond 2D COF crystallites to significantly improve their conductivity by two orders of magnitude, activating them to detect NO2 with high selectivity and sensitivity. Resonant microcantilever (RMC), grand canonical Monte Carlo (GCMC), density functional theory (DFT) and sum-frequency generation (SFG) analyses prove that 2D COFs can enrich and transfer electrons to NO2 molecules, leading to increased device conductivity. Our work gives a new approach to improving the conductivity of 2D COF films and expands the applications of 2D COF micro crystallites.
Social contact patterns relevant for infectious disease transmission in Cambodia
Authors: Leung William T.M. , Meeyai Aronrag , Holt Hannah R. , Khieu Borin , Chhay Ty , Seng Sokeyra , Pok Samkol , Chiv Phiny , Drake Tom , Rudge James W.
Published: May 2022

Social mixing patterns are key determinants of infectious disease transmission. Mathematical models parameterised with empirical data from contact pattern surveys have played an important role in understanding epidemic dynamics and informing control strategies, including for SARS-CoV-2. However, there is a paucity of data on social mixing patterns in many settings. We conducted a community-based survey in Cambodia to characterise mixing patterns and generate setting-specific contact matrices according to age and urban/rural populations. Data were collected using a diary-based approach from 2,004 participants, selected by stratified random sampling. Contact patterns were highly age-assortative, with clear intergenerational mixing between household members. Both home and school were high-intensity contact settings, with 27.7% of contacts occurring at home with non-household members. Social mixing patterns differed between rural and urban residents; rural participants tended to have more intergenerational mixing, and a higher number of contacts outside of home, work or school. Participants had low spatial mobility, with 88% of contacts occurring within 1km of the participants’ homes. These data broaden the evidence-base on social mixing patterns in low and middle-income countries and Southeast Asia, and highlight within-country heterogeneities which may be important to consider when modelling the dynamics of pathogens transmitted via close contact.
Novel six-gene prognostic signature based on colon adenocarcinoma immune-related genes
Authors: Zhou Rui , Gao ZhuoWei , Ju YongLe
Published: May 2022

Colon adenocarcinoma (COAD) is one of the most common gastrointestinal tumors worldwide, and immunotherapy is one of the most promising emerging cancer treatments. Immune genes (IGs) play a key role in tumor immunotherapy. This study aimed to determine the prognostic value of IGs in patients with COAD and establish an immune-related gene signature. Differentially expressed RNAs (DEGs), immune-related genes (DEIRGs), and transcription factors (DETFs) were screened using The Cancer Genome Atlas (TCGA), Immunology Database and Analysis Portal, InnateDB database, and Cistrome database and we constructed a regulatory network between DEIRGs and DETFs. Using Weighted Gene Co-Expression Network Analysis, we conducted five co-expressed gene modules; six hub genes were obtained using univariate and multivariate regression analysis (CD1A, CD1B, FGF9, GRP, SERPINE1, and F2RL2), leading to the construction of a risk model. Patients from TCGA database were divided into high- and low-risk groups; GSE40967 datasets were used for validation. Survival analysis, somatic gene mutations, and tumor-infiltrating immune cells differed significantly between the high- and low-risk groups. This immune-related gene signature could play an important role in guiding treatment, predicting prognosis, and potentially, future clinical applications.
Neutrophil-to-lymphocyte Ratio Is a Predictive Marker for Anti- MDA5 Positive Dermatomyositis
Authors: Liu Tao , Li Wen , Zhang Zehao , Jiang Ting , Fei Yu , Huang Jing , Xie Qibing
Published: May 2022

Objective: To correlate neutrophil-to-lymphocyte ratio (NLR) with fatality from dermatomyositis in anti-MDA5 positive patients.Method: A retrospective study in which 195 patients were enrolled was conducted. Clinical and laboratory information was collated and ratios of neutrophil to lymphocyte counts (NLR) calculated. The primary end point was all-cause death.Result: Of the 195 patients studied, all had interstitial lung disease, including 140 survivors and 55 non-survivors. An optimal NLR cut-off value of 4.86 for mortality prediction was identified. The NLR of non-survivors was significantly higher than that of survivors (p<0.001). Plasma levels of lactate dehydrogenase (LDH) and C-reactive protein (CRP) were significantly increased when NLR was greater than 4.86. Results of multivariate analysis established that NLR>4.86 was an independent predictor of mortality (HR: 2.52; 95%CI: 1.33-4.78; p=0.005). Abstinence from smoking (HR: 2.66; 95%CI: 1.33-4.78; p=0.003), emerge of rapidly progressive interstitial lung disease (RPILD; HR: 4.38; 95%CI: 2.37-8.08; p<0.001), low plasma LDH (HR: 3.82; 95%CI: 2.06-7.11; p<0.001) and appear with dyspnea (HR: 2.17; 95%CI: 1.22-3.86; p=0.009) were all protective factors predictive of survival.Conclusion: NLR is a cost-effective and widely accessible biomarker with utility for risk stratification in patients with anti-MDA5+ dermatomyositis.
Prognostic significance and biological function of SOCS3 in bladder cancer
Authors: Zhou Hui , Chen Liuxi , Liu Ying , Zhang Ruonan , Chen Bi , Feng Jiao , Liu Shuiping , Xiang Yu , Zhang Mingming , Duan Ting , Zhang Wenzheng , Tang Huifen , Yang Zuyi , Chen Xiaohui , Gu Lei , Zhang Qi
Published: May 2022

Background: Suppressor of cytokine signaling (SOCS) is a family of proteins that respond to cytokine, growth factor and other signals, and can regulate various functions of tumor cells. The purpose of our study was to investigate the prognostic performance and biological function of SOCS3 in bladder cancer. Methods: The clinical information of 80 patients with bladder cancer at Zhejiang Provincial People’s Hospital was collected and the SOCS3 protein level was detected by immunohistochemistry. The relationship between SOCS3 status and clinical parameters and the prognosis was analyzed. Besides, we explored the biological function of SOCS3 in bladder cancer. Results: The expression of SOCS3 in bladder cancer tissues was lower than that in normal tissues at the protein level and transcription level (P < 0.001). The low expression group of SOCS3 was more inclined to high staging and high grading. The prognosis of the high expression group of SOCS3 was significantly better than that of the low expression group of SOCS3 in non-muscle-invasive bladder cancer patients (P = 0.037). Also, ectopic expression of SOCS3 reduced the cell proliferation and migration ability of the bladder cancer cell line. Conclusions: Our findings imply that SOCS3 is a potential prognostic factor for non-muscle-invasive bladder cancer, and plays a tumor suppressor role in bladder cancer.
Persistence, Use of Resources and Costs in Patients Under Migraine Preventive Treatment: the PERSEC Study
Authors: Irimia Pablo , García-Azorín David , Núñez Mercedes , Díaz-Cerezo Sílvia , Polavieja Pepa García , Panni Tommaso , Sicras-Navarro Aram , Sicras-Mainar Antoni , Ciudad Antonio
Published: May 2022

Background: Migraine represents a serious burden for national health systems. However, preventive treatment is not optimally applied to reduce the severity and frequency of headache attacks and the related expenses. Our aim was to assess the persistence to traditional migraine prophylaxis available in Spain and its relationship with the healthcare resource use (HRU) and costs.Methods: Retrospective observational study with retrospective cohort design of individuals with migraine treated with oral preventive medication for the first time from 01/01/2016 to 30/06/2018. One-year follow-up information was retrieved from the Big-PacTM database. According to their one-year persistence to oral prophylaxis, two study groups were created and describe regarding HRU and healthcare direct and indirect costs using 95% confidence intervals (CI). The analysis of covariance (ANCOVA) was performed as a sensitivity analysis. Patients were considered persistent if they continued on preventive treatment until the end of the study or switched medications within 60 days or less since the last prescription. Non-persistent were those who permanently discontinued or re-initiated a treatment after 60 days.Results: 7,866 patients started preventive treatment (mean age (SD) 48.2(14.8) and 80.4% women), of whom 2,545 (32.4%) were persistent for 6 months and 2,390 (30.4%) for 12 months. Most used first-line preventive treatments were antidepressants (3,642; 46.3%) followed by antiepileptics (1,738; 22.1%) and beta-blockers (1,399; 17.8%). The acute treatments prescribed concomitantly with preventives were NSAIDs (4,530; 57.6%), followed by triptans (2,217; 28.2%). First-time preventive treatment prescribers were mostly primary care physicians (6,044; 76.8%) followed by neurologists (1,221; 15.5%). Non-persistent patients required a higher number of primary care visits (mean difference (95%CI): 3.0 (2.6;3.4)) and days of sick leave (2.7 (0.8;4.5)) than the persistent ones. The mean annual expenditure was €622 (415; 829) higher in patients who not persisted on migraine prophylactic treatment. Conclusions: In this study, we observed a high discontinuation rate for migraine prophylaxis which is related to an increase in HRU and costs for non-persistent patients. These results suggest that the treatment adherence implies not only a clinical benefit but also a reduction in HRU and costs.
Laser Spark Evolution in an Ethylene Jet in Supersonic Crossflow Configuration
Authors: Fries Dan , Ranjan Devesh , Menon Suresh
Published: May 2022

Ignition and relighting in supersonic flows is an important challenge for the design of hypersonic propulsion systems. Supersonic compressible flows of interest exhibit much larger local variations in velocity, shear, and thermodynamic state than their incompressible counterparts. Thus, it is of interest to study the relationship between ignition kernel evolution, the initial spark location, and the kernel's subsequent flow state history. We leverage the flexibility of a laser plasma ignition system to systematically explore a large set of spark locations on the symmetry plane of an ethylene jet in supersonic crossflow setup. CH* measurements are used to visualize chemically active regions and results are correlated with flow field properties derived from Mie-scattering data of the non-reacting flow field. Our study describes the laser plasma properties in detail and scrutinizes the effect of turbulent mixing and flow dilatation on ignition kernels. Finally, the results yield general guidelines for favorable ignition locations in the engineering design of chemically reactive compressible flows.
Adsorption of Hexavalent Chromium and Methylene Blue onto the Rubber Seed Shell Activated Carbon: Isotherm and Kinetic Studies
Authors: Pattarith Kongsak , Tangtubtim Supattra , Thupsuri Suphawarat
Published: May 2022

Rubber seed shell (RSS) is a low-cost agricultural by-product. It has been utilized to produce two adsorbents, rubber seed shell charcoal (RSS-CH) and rubber seed shell activated carbon (RSS-AC) for removal of hexavalent chromium (Cr(VI)) and methylene blue (MB) from aqueous solutions. The surface morphology, thermal behavior, and FTIR were characterized to confirm the successful modification on the surface of the RSS. The effects of adsorption process such as pH, initial concentration, contact time, and temperature were also studied. The adsorption isotherm results revealed that the Langmuir isotherm model was a good fit for the Cr(VI) and MB adsorption processes. The Cr(VI) adsorption kinetic was explained by pseudo-first-order kinetic model and MB adsorption was followed with pseudo-second-order kinetic model. Moreover, the maximum adsorption capacities for Cr(VI) and MB adsorptions on the RSS-AC were 156.25 and 370.37 mg/g, respectively, which showed greater than those on the RSS-CH. Thus, the RSS-AC is a suitable, alternative, and high adsorption efficiency biosorbent for the removal of heavy metal and dye contaminants in wastewater.
QC-LDPC Codes from Difference Matrices and Difference Covering Arrays
Authors: Donovan Diane , Rao Asha , Üsküplü Elif , Yazıcı Emine Şule
Published: May 2022

We give a framework for generalizing LDPC code constructions that use Transversal Designs or related structures such as mutually orthogonal Latin squares. Our construction offers a broader range of code lengths and codes rates. Similar earlier constructions rely on the existence of finite fields of order a power of a prime. In contrast the LDPC codes constructed here are based on difference matrices and difference covering arrays, structures available for any order a. They satisfy the RC constraint and have, for a odd, length a2 and rate 1 − [(4a−3)/ a2 ], and for a even, length a2 − a and rate at least 1 − [(4a−6)/ (a2 −a)]. When 3 does not divide a, these LDPC codes have stopping distance at least 8. When a is odd and both 3 and 5 do not divide a, our construction delivers an infinite family of QC-LDPC codes with minimum distance at least 10. The simplicity of the construction allows us to theoretically verify these properties and analytically determine lower bounds for the minimum distance and stopping distance of the code. The BER and FER performance of our codes over AWGN (via simulation) is at the least equivalent to codes constructed previously, while in some cases significantly outperforming them.
High-Performance Paper-based Biocathode fabricated by Screen-printing an improved Mesoporous Carbon Ink and by Oriented Immobilization of Bilirubin Oxidase
Authors: Loew Noya , Shitanda Isao , Goto Himeka , Watanabe Hikari , Mikawa Tsutomu , Tsujimura Seiya , Itagaki Masayuki
Published: May 2022

In this study, the performance of a paper-based, screen-printed biofuel cell with mesoporous MgO-templated carbon (MgOC) electrodes was improved in two steps. First, a small amount of carboxymethyl cellulose (CMC) was added to the MgOC ink. Next, the cathode was modified with bilirubin prior to immobilizing the bilirubin oxidase (BOD). The CMC increased the accessibility of the mesopores of the MgOC, and subsequently, the performance of both the bioanode and biocathode. CMC also likely increased the stability of the electrodes. The pre-modification with bilirubin improved the orientation of the BOD, which facilitated direct electron transfer. With these two steps, an open circuit potential of 0.65 V, a maximal current density of 1.94 mA cm-2, and a maximal power density of 465 μW cm-2 was achieved with lactate oxidase as bioanode enzyme and lactate as fuel. This is one of the highest reported performances for a biofuel cell.
Tuning the optical properties and thermal stability of polymeric graphitic carbon nitride (g-C3N4) with inclusion of ZnS for an emissive layer.
Authors: Nayak Debashish , Choudhary Ram Bilash
Published: May 2022

Luminescent polymeric graphitic composites have the potential to be efficient energy converters for sophisticated displays and light sources. Thermal condensation is used to synthesize g-C 3 N 4 -ZnS composites. The XRD, and FTIR analyses confirmed the synthesis of the pure host, filler, and composites. FESEM, and TEM images revealed that the ZnS nanosheets were evenly distributed over the g-C 3 N 4 sheets. The addition of ZnS improves the thermal stability of g-C 3 N 4 . The optimized gZ15 band gap is determined to be 2.98 eV with a crystallite size of 4.2 nm and a micro stain of 35.42×10 −3 . With a purity of 63.4%, gZ15 demonstrated a significant rate of recombination in the blue region. All of the improved properties demonstrated that polymeric g-C 3 N 4 -ZnS was the optimum materials for usage in the active or emissive layer of optoelectronic devices.
Association between growth hormone and psychological issues in children with short stature
Authors: Zheng Zhichao , Shan Xiaoou , Jin Jiahui , Lu Yili , Rong Xing , Luo Yue , Wu Huiping
Published: May 2022

Purpose
The use of growth hormone in children with idiopathic short stature is still controversial. We aimed to assess the benefits and downsides of growth hormone in these children at a psychological level.
Methods
The children and adolescents with short stature were recruited from the Second Affiliated Hospital of Wenzhou Medical University. They were divided into two groups: the growth hormone deficit group(GHD-G), and the idiopathic short stature group(ISS-G). The Symptom Checklist-90 scale (SCL-90) was used to evaluate the psychological situation.
Results
The results showed that the average score of GHD-G was lower than that of ISS-G, but there was no significant difference (P > 0.05). Linear regression analysis showed that the higher the growth hormone stimulation peak(GHSP), the higher the score of the SCL-90(β = 0.027, 95%CI = 0.007 to 0.047, P value = 0.00962). The results didn’t change after adjusting the factors like age, sex, and laboratory testing( β = 0.023, 95%CI = 0.003 to 0.043, P value = 0.02652). Further, when LH was more than 0.2U/L, the score of the SCL increased as the GHSP increased (β = 0.0566,95%CI = 0.0295 to 0.0837, P value = 0.0001); But when LH was less than 0.2U/L, there was not a clear relationship has been found(β=-0.0022,95%CI=-0.0268 to 0.0225, P value = 0.8631).
Conclusions
There is no evident detrimental effect of growth hormone deficit on mental health in children with short stature. Instead, increasing GHSP may hurt mental health, particularly in children before puberty. Children with idiopathic short stature who have limited height improvement following growth hormone treatment should carefully consider whether to continue using the hormone.
Predictive Nomogram for the Prediction of Recurrence of Cutaneous Squamous Cell Cancer
Authors: Zhang Wenqing , Wu Chenglong , Zhou Kaili , Cao Yu , Zhou Wange , Zhang Xue , Dan Deng
Published: May 2022

Aim: The prognosis of cutaneous squamous cell cancer (cSCC) individuals after curative resection is not satisfactory due to the recurrence with poor prognosis. We tried to identify the affecting clinical features of recurrence in cSCC patients. Methods: A total of 393 patients were retrospectively incorporated into our study. The χ test and logistic regression analysis were utilized to screen for the significant clinical indexes associated with the presence of cSCC recurrence. Recurrent nomogram was created based on the above informative clinical features to predict the probability of recurrence. The concordance index, area under curve (AUC), and calibration plots were used to assess the performance of the nomogram model and the NCCN Guidelines®. Moreover, the recurrent nomogram exhibited not only encouraging calibration ability, but also great clinical utility comparing with NCCN Guidelines®. Results: Of the 393 patients included in this study, 36 patients sufferred from recurrence. The multivariate analysis showed that exposure, tumor size, ulcer, precancerous lesions, and neural invasion at diagnosis were independent clinical factors affecting the prognosis of patients with cSCC. For the verification of results, the C-index was 0.818 respectively. The calibration curve also showed that the actual observation of recurrence was in good agreement with the prediction of the nomograms. Additionally, both DCA and CIC showed that the recurrent nomogram had good clinical application value. According to the score of each patient, the risk stratification system of cSCC patients was further established by perfectly dividing these patients into three groups, namely, low risk, medium risk, and high risk, in all queues. Conclusion: Exposure, tumor size, ulcer, precancerous lesions, and neural invasion were closely correlated with the presence of recurrence of cSCC patients. The recurrent nomogram held well predictive ability for the identification of cSCC patients with recurrence.
Application Value of Intraoperative Salvaged Autotransfusion during Posterior Lumbar Fusion: A Retrospective Study
Authors: Zhou Fenghua , Tan Xiangqian Tan , Deng Chunbo , Zhang Zhan , Ma Xun , Wang Qiushi , Xueyong Liu
Published: May 2022

Objective: To understand the application value of intraoperative salvaged autotransfusion（ISA）during posterior lumbar decompression and fusion(PLDF) and transforaminal lumbar interbody fusion（TLIF）at ≥1 surgical segments.Methods: Patients who underwent either procedure between January 2015 and January 2018 were enrolled. The patients were divided into two groups according to whether undergone ISA. Patient information, transfusion, clinical, and surgical data were analyzed for determine the clinical significance of ISA.Results: 412 patients were enrolled into two groups:181 in the ISA group and 231 in the non-ISA group. The overall requirement and volumes for allogeneic blood were significantly lower in the ISA group. TLIF at ≥2 segments resulted in significantly lower usage rate of allogeneic blood in the ISA group. During PLDF , the usage rate and volumes of allogeneic blood in the ISA group were significantly lower in both 1 and ≥2 surgical segment numbers Conclusion: The application value of ISA depends on the operation style and number of segments. During TLIF, ISA may not offer any additional clinical benefit in one segment, and the benefit in ≥ 2 segment should be invested further. During PLDF, in ISA group，the demand for allogeneic blood was reduced and patients own blood resources were effectively utilized regardless of surgical segment numbers.
Integrating omics reveals that miRNA-guided genetic regulation at the plant hormone level and defense response pathways shape resistance to Cladosporium fulvum in the tomato Cf-10-carrying line
Authors: Liu Guan , Zhang Dongye , Zhao Tingting , Yang Huanhuan , Jiang Jingbin , Li Jingfu , Zhang He , Xu Xiangyang
Published: May 2022

BackgroundInvasion of Cladosporium fulvum causes the most serious diseases affecting the reproduction of tomatoes. The Cf-10 gene has been proven to confer resistance to C. fulvum. However, how the Cf-10 gene mediates host defense gene expression and physiological changes to activate immunity in C. fulvum remains unknown. Multiple-omics studies could reveal potential regulation among different data types, providing an opportunity for dissecting the genetic regulatory mechanism underlying the Cf-10-mediated defense response.ResultsTo exploit the genetic mechanism underlying the Cf-10 gene, we performed a multiple-omics study of a resistant line carrying the Cf-10 gene and a susceptible line not carrying any resistance genes for the control and for 3 days postinoculation (dpi) of C. fulvum. By miRNA profiling, we detected 54 differentially expressed miRNAs (DE-miRNAs) between the control and 3 dpi in the resistant line, 79.63% of which were downregulated at 3 dpi. The miRNAs potentially regulate 894 genes whose functions are involved in the plant–pathogen interaction pathway and the auxin, JA, ethylene, ABA and SA signaling pathways. Transcriptome profiling revealed 1,100 differentially expressed genes (DEGs) between the control and 3 dpi in the resistant line whose functions were enriched in pathways that were potentially regulated by the DE-miRNAs. Integrating DE-miRNAs, gene expression patterns and plant-hormone metabolism profiling demonstrated that the downregulation of miRNAs at 3 dpi activated crucial resistance genes, such as Prf/PTO and RESISTANCE TO P. SYRINGAE PV MACULICOLA 1 (RPM1), to trigger host hypersensitive cell death and upregulated the receptors/critical responsive transcription factors (TFs) of plant hormones to shape immunity to the pathogen. Notably, we identified and expressionally validated that the downregulation of miR9472 would upregulate the expression of IAA-LEUCINE RESISTANT (ILR)-LIKE GENE 6 (ILL6), a gene that transforms JA to its major active component (+)-7-iso-jasmonoyl-l-isoleucine (JA-Ile), to improve the level of JA-Ile and activate JA-response genes in the Cf-10-carrying line.ConclusionThe Cf-10 gene carrying line employs a miRNA-guided “trade-off” strategy to coordinate plant growth and defense response: it expresses miRNAs to suppress invasion recognition and signal transduction pathways and reduce the level of JA and ethylene to maintain plant growth under normal conditions, but the infection of C. fulvum downregulates these miRNAs to activate the defense response pathways for acquiring disease resistance.
Use of Two Combination Creams for the Treatment of Ichthyosis and Ichthyosiform Disorders
Authors: Khalil Samar , Stephan Carla , Hasbani Divina J. , Saade Serena , Bardawil Tara , Chedraoui Adele , Abbas Ossama , Rubeiz Nelly , Kurban Mazen
Published: May 2022

Background. Treatment options for Ichthyosis and Ichthyosiform disorders are limited and often unsatisfactory. Topical cholesterol and statin have been previously used with good results. Objective. To assess the change in the disease severity score after a therapeutic regimen consisting of two combination creams: 2% cholesterol with 2% lovastatin, and 10% glycolic acid with 0.025% tretinoin and 2% ketoconazole. Methods. This study was conducted at the American University of Beirut Medical Center between March 2019 and March 2020. Pregnant women were excluded. The combination creams were used daily or as tolerated by the patients. Results. 24 patients completed the study. The median age was 7.5 years. The average percent reductions in the severity scores were 40% at 1 month (n=20), 44.8% at 2 months (n=18), and 60.3% at 3 months (n=10). Side effects consisted mainly of mild irritation which resolved upon altering the frequency of application. Limitations. Limitations of our study include the relatively small number of participants (due to the rarity of such diseases), the subjective nature of some of the scoring criteria, and the inconsistent follow-up times. Conclusion. These findings suggest a benefit from these two combination creams in the treatment of Ichthyosis and Ichthyosiform disorders.
Several properties of the matrix operators on the weighted difference sequence space derived by real numbers r and s
Authors: Candan Murat
Published: May 2022

We can describe the norm for an operator given as T:X→Y as follows: It is the most suitable value of U satisfying the following inequality ‖Tx‖_{Y}≤U‖x‖_{X} and also for the lower bound of T we can say that the value of L conforms to the following inequality ‖Tx‖_{Y}≥L‖x‖_{X}, where ‖.‖_{X} and ‖.‖_{Y} stand for the norms corresponding to the X and Y spaces, respectively. The most important characteristics of the present article lies in its computing the norms and lower bounds of those matrix operators used as weighted sequence space ℓ_{p}(w) upon a new one. This new sequence space is the generalized weighted sequence one. For this aim, the difference matrix B(r,s) and also the space consisting of those sequence whose B(r,s) transforms lie inside ℓ_{p}(w), in which r,s are taken from R.2010 Mathematics Subject Classification. 46A45, 26D15, 40G05, 47B37.
The effect of ultrasound-guided intercostal nerve block on postoperative analgesia in thoracoscopic surgery: A randomized, double-blinded, clinical trial
Authors: Li Shuo , Feng Jinteng , Fan Kun , Fan Xiaoe , Cao Shaoning , Zhang Guangjian
Published: May 2022

Background: Multimodal analgesia is accepted perioperative, intercostal nerve block (ICNB) which use ropivacaine is a kind of methods of multimodal analgesia. We aimed to explore the effect of ultrasound-guided intercostal nerve block on postoperative analgesia in thoracoscopic surgery.Methods: Patients undergoing thoracoscopic pulmonary resection were randomized to receive either intercostal nerve block (ICNB) or not under ultrasound-guidance with ropivacaine prior to surgery. Visual analog scale pain scores at rest at 0,4, 8,16,24,48,72 and 168h postoperatively, surgical outcomes and rescue analgesia requirement were also recorded. Results: VAS scores were statistically significantly lower for ICNB group compared to control group at 0, 4, 8, 16, 24 and 48h postoperatively. The duration of insertion of chest tube in ICBN group was shorter than that in control group, and the difference was statistically significant (4.69±2.14 vs 5.67±2.86, P=0.036). The postoperative hospital stay, incidence of nausea and vomiting and postoperative pulmonary infection rate in ICBN group were all lower than those in the control group, but there were no statistical differences. The frequency of rescue analgesia during 48 postoperative hours was different between the two groups (ICNB vs Control; 9.83% vs 31.03%, P=0.004).Conclusions: For patients undergoing thoracoscopic surgery, ultrasound-guided ICNB is simple, safe, and effective for providing acute postoperative pain management during the early postoperative stage.Trial registration: Chinese clinical trials: chictr.org.cn, ChiCTR1900021017. Registred on 25/01/2019.
Comparison of the Efficacy of the Two Most Used Imaging Methods in the Surgical Treatment Planning of All-On-4 and Its Variations
Authors: Tandogdu Erim , Ayali Aysa , Caymaz Mehmet Gagari
Published: May 2022

Background
This study aimed to analyze the difference between the measurements made according to certain anatomical signs of the maxillary jaw using panoramic radiography and CBCT to decide whether to use all-on-4, M-4, or V-4 configuration to prevent complications caused by incorrect measurements during the pre-surgical planning stage of the placement of implants in the all-on-4 technique.
Methods
A retrospective study was conducted with 50 patients with upper edentulous jaws suitable for the all-on-4 technique, who underwent preoperative panoramic radiography and CBCT evaluation for dental implant surgery. The shortest vertical distances between anatomical structures were measured. Measurements were made independently by two oral and maxillofacial surgeons, one experienced and the other inexperienced.
Results
A statistically significant difference was found between the mean values according to gender (p = 0,045). When the measurements made by the experienced surgeon and the inexperienced surgeon were compared, there was no significant difference between panoramic radiography and CBCT. In situations where bone measurements are required for deciding on all-on-4 or one of its configurations (M-4 and V-4), it was found that panoramic radiography gives significantly incorrect results compared to CBCT (p < 0.05).
Conclusion
CBCT is more reliable than panoramic radiography and eliminates the margin of error in the planning of all-on-4 or its variations to be made by either an experienced or an inexperienced oral surgeon.
NcRNAs-induced CAPZA1 associated with prognostic and immunological effects across Lung adenocarcinoma
Authors: Qin Ting-ting , Li Na , Li Lan , Song Qi-bin
Published: May 2022

Recently, extensive discoveries have suggested that the F-actin capping protein α1 subunit (CAPZA1) in various human tumors could elicit significantly important parts in regulating cell proliferation, metastasis and epithelial-mesenchymal transition. However, the immune-regulating role of CAPZA1 in the initiation and development of Lung adenocarcinoma (LUAD) remains incompletely clear. In our research, we firstly found that CAPZA1 serves as an oncogene in pan-cancers from TCGA data and higher CAPZA1 expression process unfavorably prognostic value in LUAD based on Starbase database and PrognoScan database. Then, in our analyses, lncRNAs AC026356.1 in LUAD acts as a competitive endogenous RNA (ceRNA) of miR-30d-5p, which might be the possible regulatory miRNA of CAPZA1 based on the Starbase database. Finally, we confirmed that CAPZA1 expression had a tightly positive correlation with immune infiltration cells, immune infiltration markers, TMB, MSI, immune score, stromal score, and immune checkpoints, indicating that CAPZA1 was a markedly reliable therapeutic target for immunological antitumor strategy. In conclusion, our investigations unveiled that CAPZA1 might function as an immune-associated biomarker in the development and treatment of LUAD, thereby acting as a promising prognostic and therapeutic target against LUAD.
Therapeutic approach with commercial supplements for pantothenate kinase-associated neurodegeneration with residual PANK2 expression levels.
Authors: Álvarez-Córdoba Mónica , Reche-López Diana , Cilleros-Holgado Paula , Talaverón-Rey Marta , Villalón-García Irene , Povea-Cabello Suleva , Suárez-Rivero Juan M , Suárez-Carrillo Alejandra , Munuera-Cabeza Manuel , Piñero-Pérez Rocío , Sánchez-Alcázar José A
Published: May 2022

Blackground: Neurodegeneration with brain iron accumulation (NBIA) is a group of rare neurogenetic disorders frequently associated with iron accumulation in the basal nuclei of the brain characterized by progressive spasticity, dystonia, muscle rigidity, neuropsychiatric symptoms, and retinal degeneration or optic nerve atrophy. Pantothenate kinase-associated neurodegeneration (PKAN) is one of the most widespread NBIA subtypes. It is caused by mutations in the gene of pantothenate kinase 2 (PANK2) that result in dysfunction in PANK2 enzyme activity, with consequent deficiency of coenzyme A (CoA) biosynthesis, as well as low levels of essential metabolic intermediates such as 4′-phosphopantetheine, a necessary cofactor for essential cytosolic and mitochondrial proteins. Methods: In this manuscript, we examined the therapeutic effectiveness of pantothenate, panthetine, antioxidants (vitamin E and omega 3) and mitochondrial function boosting supplements (L-carnitine and thiamine) in mutant PANK2 cells with residual expression levels. Results: Commercial supplements, pantothenate, pantethine, vitamin E, omega 3, carnitine and thiamine were able to eliminate iron accumulation, increase PANK2, mtACP, and NFS1 expression levels and improve pathological alterations in mutant cells with residual PANK2 expression levels. Conclusion : Our results suggest that several commercial compounds are indeed able to significantly correct the mutant phenotype in cellular models of PKAN. These compounds alone or in combinations are of common use in clinical practice and may be useful for the treatment of PKAN patients with residual enzyme expression levels.
The Inhibition of Nrf2/NQO1 pathway was involved in Polymyxin B Exerts Nephrotoxicity Effects in vivo and in vitro
Authors: XIAO GuiYing , Yuan Fang , Xiang Debiao , Xiong Liguang , Deng Yang , Li Yuan , Li Xin
Published: May 2022

Polymyxin B (PMB) is a Polypeptide antibiotic widely applied in multidrug-resistant Gram-negative bacteria. However, nephrotoxicity is one of the serious adverse effects that limit its clinical use. Therefore, clarification of the molecular mechanism of PMB-induced renal injury appears to be particularly important. Our study aimed to explore the possible mechanism of PMB-induced nephrotoxicity in vivo and in vitro. Mice were treated with PMB to construct models of kidney injury. The antioxidant capacity was assessed by measuring the activity of SOD and CAT and the contents of GSH and MDA, and the Nrf2/NQO1 pathway was examined after PMB treatment in NRK-52E cells and mice. Finally, we assessed the expression of apoptosis-related genes and proteins (Bax, Bcl-2, Caspase-3, Caspase-9) through qPCR and WB assay. The study verified PMB-induced nephrotoxicity in mice and NRK-52E cells by a dose- and time-dependent manners. PMB treatment significantly down-regulated the expression of Nrf2 and its downstream target genes NQO1 and up-regulated the apoptosis-related protein expression. In summary, our results suggested PMB induced oxidative stress damage by inhibiting the Nrf2/NQO1 pathway and promoting apoptosis in kidney tissue.
Matrix Indicators for Assessing the Quality of Municipal Plans for Integrated Management of Urban Solid Waste
Authors: Neves Raisa Rodrigues , Fernandes Lindemberg Lima , Braga Risete Maria Queiroz Leão , Crispim Diêgo Lima , Lisboa Isabela de Aviz , Sá João Henrique Macedo
Published: May 2022

The correct management of solid waste is still considered an obstacle for Brazilian municipal realities. In this sense, the National Policy on Solid Waste (NPSW) brings the Integrated Municipal Solid Waste Management Plans (IMSWMP) as a fundamental instrument to guide decision-making. From this, this article sought to propose indicators to assess the quality of elaboration of these documents. The methodological steps were composed of 3 parts: literature review to build a matrix of 40 indicators and 7 dimensions; matrix validation with the attribution of grades to the descriptors and weights to the dimensions considered by the Delphi Method; and calculation of the Quality Index of Integrated Municipal Solid Waste Management Plans applied to the 16 municipalities in the State of Pará. The proposed matrix was able to identify the dimensions and indicators that need improvement, contributing to the elaboration of consistent IMSWMP and for the continuity regarding the advances of the IMSWMP considering the municipal limitations and government changes.
Comparison of AliveCor KardiaMobile Six-Lead ECG with Standard ECG in Pediatric Patients
Authors: Girvin Zachary P. , Silver Eric S. , Liberman Leonardo
Published: May 2022

Background: The AliveCor KardiaMobile (ACKM) is a remote electrocardiogram (ECG) monitoring device. Little research has been conducted on its accuracy with pediatric patients. This prospective study aims to compare the ACKM six-lead device with a standard fifteen-lead ECG in measuring the QTc, QRS, and axis in pediatric patients.Methods: Pediatric patients ages 5 to 21 years were enrolled prospectively to have their ECG recorded using an ACKM six-lead device following a recording with the standard fifteen-lead ECG. A pediatric electrophysiologist measured the QTc, QRS interval, and QRS axis for both ECGs. Bland-Altman analysis was performed to assess agreement among measurements.Results: The study included 141 patients. The mean age was 12.3 ± 4.4 years. Average heart rate was 79 ± 16 bpm. The mean difference in the QTc measurements for a paired standard ECG and ACKM was -0.6 ms [95% confidence interval -48 to 47 ms]. Of the ACKM QTc measurements, 117 (83%) were within 30 ms of the standard ECG. The mean difference in paired QRS measurements was -1.3 ms [95% confidence interval -23 to 21 ms]. Of the ACKM QRS measurements, 134 (95%) were within 20 ms of the standard ECG. The measured axis was the same for 84% of ACKM and standard ECGs. Conclusions: Over 80% of the ACKM six-lead ECGs produced QTc, QRS, and axis deviation measurements within a clinically useful range of the standard ECG. However, it is not accurate enough to be used consistently in place of a standard ECG for QTc and QRS measurement for pediatric patients.