bluuebunny/crossref_metadata_2025 huggingface dataset:
A Survey of Methods and Input Data Types for House Price Prediction
Authors: Geerts Margot , vanden Broucke Seppe , De Weerdt Jochen
Published: May 2023

Predicting house prices is a challenging task that many researchers have attempted to address. As accurate house prices allow better informing parties in the real estate market, improving housing policies and real estate appraisal, a comprehensive overview of house price prediction strategies is valuable for both research and society. In this work, we present a systematic literature review in order to provide insights with regard to the data types and modeling approaches that have been utilized in the current body of research. As such, we identified 93 articles published between 1992 and 2021 presenting a particular technique for house price prediction. Subsequently, we scrutinized these works and scored them according to model and data novelty. A cluster analysis allowed mapping of the property valuation domain and identification of trends. Although conventional methods and traditional input data remain predominant, house price prediction research is slowly adopting more advanced techniques and innovative data sources. In addition, we identify opportunities to include more advanced input data types such as unstructured data and complex spatial data and to introduce deep learning and tailored methods, which could guide further research.
Simple Coumarins from Peucedanum luxurians Fruits: Evaluation of Anxiolytic Activity and Influence on Gene Expression Related to Anxiety in Zebrafish Model
Authors: Widelski Jarosław , Kasica Natalia , Maciąg Monika , Luca Simon Vlad , Budzyńska Barbara , Fondai Dafina , Podlasz Piotr , Skalicka-Woźniak Krystyna
Published: May 2023

Anxiety is one of the most common central nervous system disorders, affecting at least one-quarter of the worldwide population. The medications routinely used for the treatment of anxiety (mainly benzodiazepines) are a cause of addiction and are characterized by many undesirable side effects. Thus, there is an important and urgent need for screening and finding novel drug candidates that can be used in the prevention or treatment of anxiety. Simple coumarins usually do not show side effects, or these effects are much lower than in the case of synthetic drugs acting on the central nervous system (CNS). This study aimed to evaluate the anxiolytic activity of three simple coumarins from Peucedanum luxurians Tamamsch, namely officinalin, stenocarpin isobutyrate, and officinalin isobutyrate, in a 5 dpf larval zebrafish model. Moreover, the influence of the tested coumarins on the expression of genes involved in the neural activity (c-fos, bdnf) or dopaminergic (th1), serotoninergic (htr1Aa, htr1b, htr2b), GABA-ergic (gabarapa, gabarapb), enkephalinergic (penka, penkb), and galaninergic (galn) neurotransmission was assessed by quantitative PCR. All tested coumarins showed significant anxiolytic activity, with officinalin as the most potent compound. The presence of a free hydroxyl group at position C-7 and the lack of methoxy moiety at position C-8 might be key structural features responsible for the observed effects. In addition, officinalin and its isobutyrate upregulated the expression of genes involved in neurotransmission and decreased the expression of genes connected with neural activity. Therefore, the coumarins from P. luxurians might be considered as promising drug candidates for the therapy of anxiety and related disorders.
Activin A Limits VEGF-Induced Permeability via VE-PTP
Authors: Baccouche Basma , Lietuvninkas Lina , Kazlauskas Andrius
Published: May 2023

The clinical success of neutralizing vascular endothelial growth factor (VEGF) has unequivocally identified VEGF as a driver of retinal edema that underlies a variety of blinding conditions. VEGF is not the only input that is received and integrated by the endothelium. For instance, the permeability of blood vessels is also regulated by the large and ubiquitously expressed transforming growth factor beta (TGF-β) family. In this project, we tested the hypothesis that members of the TGF-β family influence the VEGF-mediated control of the endothelial cell barrier. To this end, we compared the effect of bone morphogenetic protein-9 (BMP-9), TGF-β1, and activin A on the VEGF-driven permeability of primary human retinal endothelial cells. While BMP-9 and TGF-β1 had no effect on VEGF-induced permeability, activin A limited the extent to which VEGF relaxed the barrier. This activin A effect was associated with the reduced activation of VEGFR2 and its downstream effectors and an increased expression of vascular endothelial tyrosine phosphatase (VE-PTP). Attenuating the expression or activity of VE-PTP overcame the effect of activin A. Taken together, these observations indicate that the TGF-β superfamily governed VEGF-mediated responsiveness in a ligand-specific manner. Furthermore, activin A suppressed the responsiveness of cells to VEGF, and the underlying mechanism involved the VE-PTP-mediated dephosphorylation of VEGFR2.
Impact of Functional Polymorphisms on Drug Survival of Biological Therapies in Patients with Moderate-to-Severe Psoriasis
Authors: Membrive-Jiménez Cristina , Pérez-Ramírez Cristina , Arias-Santiago Salvador , Richetta Antonio Giovanni , Ottini Laura , Pineda-Lancheros Laura Elena , Ramírez-Tortosa Maria del Carmen , Jiménez-Morales Alberto
Published: May 2023

Biological therapies (BTs) indicated for psoriasis are highly effective; however, not all patients obtain good results, and loss of effectiveness is the main reason for switching. Genetic factors may be involved. The objective of this study was to evaluate the influence of single-nucleotide polymorphisms (SNPs) on the drug survival of tumor necrosis factor inhibitors (anti-TNF) medications and ustekinumab (UTK) in patients diagnosed with moderate-to-severe psoriasis. We conducted an ambispective observational cohort study that included 379 lines of treatment with anti-TNF (n = 247) and UTK (132) in 206 white patients from southern Spain and Italy. The genotyping of the 29 functional SNPs was carried out using real-time polymerase chain reaction (PCR) with TaqMan probes. Drug survival was evaluated with Cox regression and Kaplan–Meier curves. The multivariate analysis showed that the HLA-C rs12191877-T (hazard ratio [HR] = 0.560; 95% CI = 0.40–0.78; p = 0.0006) and TNF-1031 (rs1799964-C) (HR = 0.707; 95% CI = 0.50–0.99; p = 0.048) polymorphisms are associated with anti-TNF drug survival, while TLR5 rs5744174-G (HR = 0.589; 95% CI = 0.37–0.92; p = 0.02), CD84 rs6427528-GG (HR = 0.557; 95% CI = 0.35–0.88; p = 0.013) and PDE3A rs11045392-T together with SLCO1C1 rs3794271-T (HR = 0.508; 95% CI = 0.32–0.79; p = 0.002) are related to UTK survival. The limitations are the sample size and the clustering of anti-TNF drugs; we used a homogeneous cohort of patients from 2 hospitals only. In conclusion, SNPs in the HLA-C, TNF, TLR5, CD84, PDE3A, and SLCO1C1 genes may be useful as biomarkers of drug survival of BTs indicated for psoriasis, making it possible to implement personalized medicine that will reduce financial healthcare costs, facilitate medical decision-making and improve patient quality of life. However, further pharmacogenetic studies need to be conducted to confirm these associations.
Bioinformatics Identification of Regulatory Genes and Mechanism Related to Hypoxia-Induced PD-L1 Inhibitor Resistance in Hepatocellular Carcinoma
Authors: Huang Mohan , Yang Sijun , Tai William Chi Shing , Zhang Lingfeng , Zhou Yinuo , Cho William Chi Shing , Chan Lawrence Wing Chi , Wong Sze Chuen Cesar
Published: May 2023

The combination of a PD-L1 inhibitor and an anti-angiogenic agent has become the new reference standard in the first-line treatment of non-excisable hepatocellular carcinoma (HCC) due to the survival advantage, but its objective response rate remains low at 36%. Evidence shows that PD-L1 inhibitor resistance is attributed to hypoxic tumor microenvironment. In this study, we performed bioinformatics analysis to identify genes and the underlying mechanisms that improve the efficacy of PD-L1 inhibition. Two public datasets of gene expression profiles, (1) HCC tumor versus adjacent normal tissue (N = 214) and (2) normoxia versus anoxia of HepG2 cells (N = 6), were collected from Gene Expression Omnibus (GEO) database. We identified HCC-signature and hypoxia-related genes, using differential expression analysis, and their 52 overlapping genes. Of these 52 genes, 14 PD-L1 regulator genes were further identified through the multiple regression analysis of TCGA-LIHC dataset (N = 371), and 10 hub genes were indicated in the protein–protein interaction (PPI) network. It was found that POLE2, GABARAPL1, PIK3R1, NDC80, and TPX2 play critical roles in the response and overall survival in cancer patients under PD-L1 inhibitor treatment. Our study provides new insights and potential biomarkers to enhance the immunotherapeutic role of PD-L1 inhibitors in HCC, which can help in exploring new therapeutic strategies.
Somatosensory-Evoked Early Sharp Waves in the Neonatal Rat Hippocampus
Authors: Gainutdinov Azat , Shipkov Dmitrii , Sintsov Mikhail , Fabrizi Lorenzo , Nasretdinov Azat , Khazipov Roustem , Valeeva Guzel
Published: May 2023

The developing entorhinal–hippocampal system is embedded within a large-scale bottom-up network, where spontaneous myoclonic movements, presumably via somatosensory feedback, trigger hippocampal early sharp waves (eSPWs). The hypothesis, that somatosensory feedback links myoclonic movements with eSPWs, implies that direct somatosensory stimulation should also be capable of evoking eSPWs. In this study, we examined hippocampal responses to electrical stimulation of the somatosensory periphery in urethane-anesthetized, immobilized neonatal rat pups using silicone probe recordings. We found that somatosensory stimulation in ~33% of the trials evoked local field potential (LFP) and multiple unit activity (MUA) responses identical to spontaneous eSPWs. The somatosensory-evoked eSPWs were delayed from the stimulus, on average, by 188 ms. Both spontaneous and somatosensory-evoked eSPWs (i) had similar amplitude of ~0.5 mV and half-duration of ~40 ms, (ii) had similar current-source density (CSD) profiles, with current sinks in CA1 strata radiatum, lacunosum-moleculare and DG molecular layer and (iii) were associated with MUA increase in CA1 and DG. Our results indicate that eSPWs can be triggered by direct somatosensory stimulations and support the hypothesis that sensory feedback from movements is involved in the association of eSPWs with myoclonic movements in neonatal rats.
No Substrate Left behind—Mining of Shotgun Proteomics Datasets Rescues Evidence of Proteolysis by SARS-CoV-2 3CLpro Main Protease
Authors: Bell Peter A. , Overall Christopher M.
Published: May 2023

Proteolytic processing is the most ubiquitous post-translational modification and regulator of protein function. To identify protease substrates, and hence the function of proteases, terminomics workflows have been developed to enrich and detect proteolytically generated protein termini from mass spectrometry data. The mining of shotgun proteomics datasets for such ‘neo’-termini, to increase the understanding of proteolytic processing, is an underutilized opportunity. However, to date, this approach has been hindered by the lack of software with sufficient speed to make searching for the relatively low numbers of protease-generated semi-tryptic peptides present in non-enriched samples viable. We reanalyzed published shotgun proteomics datasets for evidence of proteolytic processing in COVID-19 using the recently upgraded MSFragger/FragPipe software, which searches data with a speed that is an order of magnitude greater than many equivalent tools. The number of protein termini identified was higher than expected and constituted around half the number of termini detected by two different N-terminomics methods. We identified neo-N- and C-termini generated during SARS-CoV-2 infection that were indicative of proteolysis and were mediated by both viral and host proteases—a number of which had been recently validated by in vitro assays. Thus, re-analyzing existing shotgun proteomics data is a valuable adjunct for terminomics research that can be readily tapped (for example, in the next pandemic where data would be scarce) to increase the understanding of protease function and virus–host interactions, or other diverse biological processes.
Live Cells Imaging and Comparative Phosphoproteomics Uncover Proteins from the Mechanobiome in Entamoeba histolytica
Authors: Jhingan Gagan Deep , Manich Maria , Olivo-Marin Jean-Christophe , Guillen Nancy
Published: May 2023

Entamoeba histolytica is a protozoan parasite and the causative agent of amoebiasis in humans. This amoeba invades human tissues by taking advantage of its actin-rich cytoskeleton to move, enter the tissue matrix, kill and phagocyte the human cells. During tissue invasion, E. histolytica moves from the intestinal lumen across the mucus layer and enters the epithelial parenchyma. Faced with the chemical and physical constraints of these diverse environments, E. histolytica has developed sophisticated systems to integrate internal and external signals and to coordinate cell shape changes and motility. Cell signalling circuits are driven by interactions between the parasite and extracellular matrix, combined with rapid responses from the mechanobiome in which protein phosphorylation plays an important role. To understand the role of phosphorylation events and related signalling mechanisms, we targeted phosphatidylinositol 3-kinases followed by live cell imaging and phosphoproteomics. The results highlight 1150 proteins, out of the 7966 proteins within the amoebic proteome, as members of the phosphoproteome, including signalling and structural molecules involved in cytoskeletal activities. Inhibition of phosphatidylinositol 3-kinases alters phosphorylation in important members of these categories; a finding that correlates with changes in amoeba motility and morphology, as well as a decrease in actin-rich adhesive structures.
Determinants of COVID-19 Disease Severity–Lessons from Primary and Secondary Immune Disorders including Cancer
Authors: Solimando Antonio G. , Bittrich Max , Shahini Endrit , Albanese Federica , Fritz Georg , Krebs Markus
Published: May 2023

At the beginning of the COVID-19 pandemic, patients with primary and secondary immune disorders—including patients suffering from cancer—were generally regarded as a high-risk population in terms of COVID-19 disease severity and mortality. By now, scientific evidence indicates that there is substantial heterogeneity regarding the vulnerability towards COVID-19 in patients with immune disorders. In this review, we aimed to summarize the current knowledge about the effect of coexistent immune disorders on COVID-19 disease severity and vaccination response. In this context, we also regarded cancer as a secondary immune disorder. While patients with hematological malignancies displayed lower seroconversion rates after vaccination in some studies, a majority of cancer patients’ risk factors for severe COVID-19 disease were either inherent (such as metastatic or progressive disease) or comparable to the general population (age, male gender and comorbidities such as kidney or liver disease). A deeper understanding is needed to better define patient subgroups at a higher risk for severe COVID-19 disease courses. At the same time, immune disorders as functional disease models offer further insights into the role of specific immune cells and cytokines when orchestrating the immune response towards SARS-CoV-2 infection. Longitudinal serological studies are urgently needed to determine the extent and the duration of SARS-CoV-2 immunity in the general population, as well as immune-compromised and oncological patients.
miR-486 Responds to Apoptosis and Autophagy by Repressing SRSF3 Expression in Ovarian Granulosa Cells of Dairy Goats
Authors: Liu Shujuan , Bu Qiqi , Tong Jiashun , Wang Zhanhang , Cui Jiuzeng , Cao Heran , Ma Haidong , Cao Binyun , An Xiaopeng , Song Yuxuan
Published: May 2023

The accumulation of ovarian granulosa cell (GC) apoptosis underlies follicular atresia. By comparing the previous sequencing results, miR-486 was found to be differentially expressed at higher levels in the monotocous goat than in the polytocous goat. Unfortunately, the miRNA-mediated mechanisms by which the GC fate is regulated are unknown in Guanzhong dairy goats. Therefore, we investigated miR-486 expression in small and large follicles, as well as its impact on normal GC survival, apoptosis and autophagy in vitro. Here, we identified and characterized miR-486 interaction with Ser/Arg-rich splicing factor 3 (SRSF3) using luciferase reporter analysis, detecting its role in GC survival, apoptosis and autophagy regulation through qRT-PCR, Western blot, CCK-8, EdU, flow cytometry, mitochondrial membrane potential and monodansylcadaverine, etc. Our findings revealed prominent effects of miR-486 in the regulation of GC survival, apoptosis and autophagy by targeting SRSF3, which might explain the high differential expression of miR-486 in the ovaries of monotocous dairy goats. In summary, this study aimed to reveal the underlying molecular mechanism of miR-486 regulation on GC function and its effect on ovarian follicle atresia in dairy goats, as well as the functional interpretation of the downstream target gene SRSF3.
Molecular Mechanisms of Retinal Degeneration and How to Avoid It
Authors: Kovács-Öller Tamás , Völgyi Béla
Published: May 2023

Vision is the most important sensory modality in vertebrates in general, and as such, it is the most feared sense to lose [...]
Proposal of the Indonesian Framework for Telecommunications Infrastructure Based on Network and Socioeconomic Indicators
Authors: Situmorang Anna Christina , Suryanegara Muhammad , Gunawan Dadang , Juwono Filbert H.
Published: May 2023

In Indonesia, there is still a disparity in telecommunications access, with most rural areas experiencing “no signal” or “blank spots.” In contrast, urban areas enjoy modern and societally-beneficial technologies. A comprehensive framework is needed to address the disparity in telecommunications access between “rich” and “poor” groups in urban and rural/remote areas, respectively. This paper proposes a framework, built by the mathematical model, that can be used as a reference for the Indonesian government in constructing the nation’s telecommunications infrastructure. The framework categorizes Indonesian administrative regions into four grids: Grid #1: “fostered” districts; Grid #2: “developing” districts; Grid #3: “developed” districts; and Grid #4: “independent-advanced” districts. To determine where each district falls in these grids, we propose a novel statistical approach using 17 indicators involving a telecommunications network and socioeconomic factors. The proposed framework results in a grid visualization of 7232 districts in Indonesia. Finally, as this paper is replete with academic research approaches and mathematical model perspectives, it is expected that the results may be a valuable input to the development of the country’s telecommunications policy.
Temperature-Driven Synthesis of 1D Fe2O3@3D Graphene Composite Applies as Anode of Lithium-Ion Batteries
Authors: Zhu Shengyuan , Li Ruizhi , Xu Jiapeng , Yang Liu , Zhou Yingke
Published: May 2023

A series of Fe2O3-anchored three-dimensional graphene (3DG) composites are synthesized via hydrothermal and annealing methods. The Fe2O3 nanocrystals in composites display nanocubes, one-dimensional (1D) nanorods and ellipsoids at hydrothermal temperatures of 120 °C, 150 °C and 180 °C, respectively. Notably, the composite synthesized at 150 °C shows 1D Fe2O3 uniformly embedded in 3DG, forming an interpenetrating 1D-3D (three-dimensional) structure. This combined structure is beneficial in improving the electrochemical stability and accelerating the Li+ diffusion rate. When used as anode for lithium-ion batteries (LIBs), the optimized 1D-3D Fe2O3@3DG composite delivers a reversible specific capacity of 1041 mAh g−1 at 0.1 A g−1 and maintains a high reversible specific capacity of 775 mAh g−1 after 200 cycles. The superior electrochemical properties of Fe2O3@3DG are a result of the stable interpenetrate structure, enhanced conductivity, and buffered volume change. These results suggest that Fe2O3@3DG composites have significant potential as advanced anode materials for LIBs and the combined 1D-3D structure also provides inspiration for other electrode material structure design.
Effects of Irradiation on Biology and Mating Behaviour of Wild Males of Brown Marmorated Stink Bug Using a 6 MV Medical Linear Accelerator
Authors: Roselli Gerardo , Anfora Gianfranco , Suckling David Maxwell , Mazzoni Valerio , Vanoni Valentina , Menegotti Loris , Fellin Lorenzo , Rossi Stacconi Marco Valerio , Ioriatti Claudio , Cristofaro Massimo
Published: May 2023

The brown marmorated stink bug, Halyomorpha halys, is a pentatomid bug of Eastern Asian origin that became an economically relevant pest in the Eurasian and American continents. Management of this species is limited to use of chemical insecticides: an inefficient method due to the strong adaptability of the target pest. The sterile insect technique (SIT) is potentially a valid tactic in the search for nontoxic alternatives. In this work, we investigated the suitability of mass-trapped overwintering males, collected during the aggregation phase before the winter diapause, for their release as competitive sterile males in an SIT programme. Differently from previous studies, irradiation was applied with a linear accelerator device that produced high-energy photons. Following a similar scientific protocol with newly emerged irradiated males, the effects of X-ray irradiation on physiological parameters (longevity, fecundity and fertility) were assessed. In addition, behavioural bioassays were carried out in no-choice conditions to evaluate if irradiation interferes with mating processes. The results are very encouraging; the effects of the irradiation at 32 Gy did not differ from the controls in the longevity or fecundity of the exposed overwintering adults. The hatching rate of the eggs laid by the fertile females that had mated with the irradiated males was less than 5%. The results of behavioural bioassays showed that the irradiation did not cause a significant impact on the quality of the sterile males. More research is warranted to evaluate the mating competitiveness of sterile males in semi-field and field conditions.
The Intriguing Role of Hypoxia-Inducible Factor in Myocardial Ischemia and Reperfusion: A Comprehensive Review
Authors: Heck-Swain Ka-Lin , Koeppen Michael
Published: May 2023

Hypoxia-inducible factors (HIFs) play a crucial role in cellular responses to low oxygen levels during myocardial ischemia and reperfusion injury. HIF stabilizers, originally developed for treating renal anemia, may offer cardiac protection in this context. This narrative review examines the molecular mechanisms governing HIF activation and function, as well as the pathways involved in cell protection. Furthermore, we analyze the distinct cellular roles of HIFs in myocardial ischemia and reperfusion. We also explore potential therapies targeting HIFs, emphasizing their possible benefits and limitations. Finally, we discuss the challenges and opportunities in this research area, underscoring the need for continued investigation to fully realize the therapeutic potential of HIF modulation in managing this complex condition.
Burden and Risk Factors of Postpartum Depression in Southwest Saudi Arabia
Authors: Alshahrani Safar Abadi Saeed Al-Saleem , Al-Saleh Norah Fayz , Siddiqui Aesha Farheen , Khalil Shamsun Nahar , Abadi Mohammed , AlHefdhi Hayfa A. , Al-Qadi Sahar Awad , Asiri Abdullah Ahmad
Published: May 2023

Objectives: The burden of postpartum depression (PPD) is significant because it remains unrecognized, and it not only affects the mother adversely but also has a negative consequence on the family life and the development of the infant. The aim of the study was to measure the prevalence of PPD and identify the risk factors of PPD among mothers attending the well-baby clinic of six Primary Health Care centers in Abha city, Southwest Saudi Arabia. Materials and Methods: A total of 228 Saudi women having a child aged between two weeks to one year were recruited in the study by using a consecutive sampling technique. The Arabic version of the Edinburgh Postnatal Depression Scale (EPDS) was used as a screening tool to measure the prevalence of PPD. The mothers were also inquired about their socio-demographic characteristics and risk factors. Results: The prevalence rate of postpartum depression was 43.4%. Family conflict, and lack of support by spouse and family during pregnancy were found to be the strongest predictors of developing PPD. Women who had reported family conflict were at six times higher risk for developing PPD compared to those who did not have a family conflict (aOR = 6.5, 95% CI = 2.3–18.4). Women who reported a lack of spousal support during pregnancy encountered 2.3 fold increased risk for PPD (aOR = 2.3, 95% CI = 1.0–4.8) and women who had not received family support during pregnancy period were more than three times (aOR = 3.5, 95 % CI 1.6–7.7) likely to experience PPD. Conclusion: The risk of PPD among Saudi postnatal women was high. PPD screening should be an integral part of postnatal care. Awareness of women, spouses and families about potential risk factors can be a preventive strategy. The early identification of high-risk women during the antenatal and post-natal period could help to prevent this condition.
Clinical Risk Factors for Dysphagia and Esophageal Dysmotility in Systemic Sclerosis
Authors: Hara Mariko , Ueha Rumi , Sato Taku , Goto Takao , Yoshizaki Ayumi , Sumida Hayakazu , Sato Shinichi , Yamasoba Tatsuya
Published: May 2023

Systemic sclerosis (SSc) is often associated with dysphagia and esophageal dysmotility; however, only a few clinical studies on this topic have been conducted. Patients with SSc who underwent swallowing examinations and esophagography at our institution between 2010 and 2022 were included. A retrospective evaluation of the patients’ backgrounds, autoantibody positivity, swallowing function, and esophageal motility was performed using medical charts. The association between dysphagia and esophageal dysmotility in patients with SSc and respective risk factors was investigated. Data were collected from 50 patients. Anti-topoisomerase I antibodies (ATA) and anti-centromere antibodies (ACA) were detected in 21 (42%) and 11 (22%) patients, respectively. Dysphagia was present in 13 patients (26%), and esophageal dysmotility in 34 patients (68%). ATA-positive patients had a higher risk for dysphagia (p = 0.027); ACA-positive patients had a significantly lower risk (p = 0.046). Older age and laryngeal sensory deficits were identified as risk factors for dysphagia; however, no risk factors for esophageal dysmotility were identified. No correlation was found between dysphagia and esophageal dysmotility. Esophageal dysmotility is more common in patients with SSc than in those with dysphagia. Autoantibodies can be predictors of dysphagia, and dysphagia must be carefully considered in ATA-positive and elderly patients with SSc.
Special Issue: Latest Advances in Delivery and Outcomes of Cardiac Resynchronization Therapy and Conduction System Pacing
Authors: Maass Alexander H. , Daniëls Fenna , Roseboom Eva , Vernooy Kevin , Rienstra Michiel
Published: May 2023

Cardiac Resynchronization Therapy (CRT) is an established technique to improve morbidity and mortality in selected heart failure patients [...]
Cellular and Molecular Mechanisms Underlying Tricuspid Valve Development and Disease
Authors: Salerno Nadia , Panuccio Giuseppe , Sabatino Jolanda , Leo Isabella , Torella Michele , Sorrentino Sabato , De Rosa Salvatore , Torella Daniele
Published: May 2023

Tricuspid valve (TV) disease is highly prevalent in the general population. For ages considered “the forgotten valve” because of the predominant interest in left-side valve disease, the TV has now received significant attention in recent years, with significant improvement both in diagnosis and in management of tricuspid disease. TV is characterized by complex anatomy, physiology, and pathophysiology, in which the right ventricle plays a fundamental role. Comprehensive knowledge of molecular and cellular mechanisms underlying TV development, TV disease, and tricuspid regurgitation-related right-ventricle cardiomyopathy is necessary to enhance TV disease understanding to improve the ability to risk stratify TR patients, while also predicting valve dysfunction and/or response to tricuspid regurgitation treatment. Scientific efforts are still needed to eventually decipher the complete picture describing the etiopathogenesis of TV and TV-associated cardiomyopathy, and future advances to this aim may be achieved by combining emerging diagnostic imaging modalities with molecular and cellular studies. Overall, basic science studies could help to streamline a new coherent hypothesis underlying both the development of TV during embryogenesis and TV-associated disease and its complications in adult life, providing the conceptual basis for the ultimate and innovative field of valve repair and regeneration using tissue-engineered heart valves.
Dietary Behaviors and Beliefs in Patients with Inflammatory Bowel Disease
Authors: Godala Małgorzata , Gaszyńska Ewelina , Durko Łukasz , Małecka-Wojciesko Ewa
Published: May 2023

Introduction: Due to a lack of clear dietary guidelines, patients with inflammatory bowel disease (IBD) self-impose dietary restrictions based on their own nutritional experiences. The aim of this study was to investigate dietary perceptions and behavior in IBD patients. Materials and methods: A total of 82 patients (48 with Crohn’s disease and 34 with ulcerative colitis) participated in this prospective, questionnaire-based study. Based on a literature review, the questionnaire was developed to investigate dietary beliefs, behaviors and food exclusions during IBD relapses and remission. Results: The majority of patients (85.4%) believed that diet can be a trigger factor for IBD relapses, and 32.9% believed that diet initiates the disease. The majority of patients (81.7%) believed that they should eliminate some products from their diets. The most often-pointed-out products were spicy and fatty foods, raw fruits and vegetables, alcohol, leguminous foods, cruciferous vegetables, dairy products and milk. Most patients (75%) modified their diets after diagnosis, and 81.7% imposed food restrictions to prevent IBD relapses. Conclusions: The majority of patients avoided certain foods during relapses as well as to maintain remission of IBD, basing this on their own beliefs, inconsistently with current scientific knowledge. Patient education should be a key determinant in IBD control.
Occurrence of Severe Arrhythmias in Patients with Non-ST Elevation Acute Coronary Syndrome (NSTE-ACS): A Retrospective Study
Authors: Wilmé Valérie , Harscoat Sébastien , Séverac François , Carmona Adrien , Le Borgne Pierrick , Bilbault Pascal , Morel Olivier , Kepka Sabrina
Published: May 2023

Background: Non-ST elevation acute coronary syndrome (NSTE-ACS) is one of the most frequent manifestations of coronary artery disease. The occurrence of serious heart rhythm disorders (SHRDs) in NSTE-ACS is not well documented. However, continuous heart rhythm monitoring is recommended during the initial management of NSTE-ACS. The targeted monitoring of patients at greater risk for SHRDs could facilitate patients’ care in emergency departments (EDs) where the flow of patients is continuously increasing. Methods: This retrospective single-center study included 480 patients from emergency and cardiology departments within the Strasbourg University Hospital between 1 January 2019 and 31 December 2020. The objective was to estimate the frequency of the occurrence of SHRDs among patients with NSTE-ACS. The secondary objective was to highlight the factors associated with a higher risk of SHRDs. Results: The proportion of SHRDs during the first 48 h of hospital care was 2.3% (CI95%: 1.2–4.1%, n = 11). Two time periods were considered: before coronary angiography (1.0%), and during, or after coronary angiography (1.3%). In the first group, two patients required immediate treatment (0.4% of the patients) and no death occurred. In the univariate analysis, the variables significantly associated with SHRDs were age, anticoagulant medication, a decrease in glomerular filtration rate, plasmatic hemoglobin, and left ventricle ejection fraction (LVEF), and an increase in plasmatic troponin, BNP, and CRP levels. In the multivariable analysis, plasmatic hemoglobin > 12 g/dL seemed to be a protective factor for SHRDs. Conclusions: In this study, SHRDs were rare and, most often, spontaneously resolved. These data challenge the relevance of systematic rhythm monitoring during the initial management of patients with NSTE-ACS.
The Safety and Efficacy of Ultrasound-Accelerated Catheter-Directed Thrombolysis in Patients with Intermediate–High-Risk Pulmonary Embolism: Bo-NE-Experience
Authors: Al-Terki Hani , Mügge Andreas , Gotzmann Michael , Tiyerili Vedat , Klein Friederike , Franz Marcus , Möbius-Winkler Sven , Elhakim Abdelrahman
Published: May 2023

Ultrasound-accelerated thrombolysis (USAT) is an advanced interventional therapy for patients with intermediate–high-risk pulmonary embolism (PE) who deteriorated on anticoagulation or for high-risk patients for whom systemic thrombolysis is contraindicated. The aim of this study is to investigate the safety and efficacy of this therapy with a focus on the improvement of vital signs and laboratory parameters. Seventy-nine patients with intermediate–high-risk PE were treated with USAT from August 2020 to November 2022. The therapy significantly decreased the mean RV/LV ratio from 1.2 ± 0.22 to 0.9 ± 0.2 (p < 0.001) as well as the mean PAPs from 48.6 ± 11 to 30.1 ± 9.0 mmHg (p < 0.001). The respiratory and heart rate decreased significantly (p < 0.001). Serum creatinine decreased significantly from 1.0 ± 0.35 to 0.9 ± 0.3 (p < 0.001). There were 12 access-associated complications, which could be treated conservatively. One patient had haemothorax after the therapy and had to be operated on. USAT is an effective therapy for patients with intermediate–high-risk PE, with favourable hemodynamic, clinical, and laboratory outcomes.
Computer-Based Mechanobiological Fracture Healing Model Predicts Non-Union of Surgically Treated Diaphyseal Femur Fractures
Authors: Degenhart Christina , Engelhardt Lucas , Niemeyer Frank , Erne Felix , Braun Benedikt , Gebhard Florian , Schütze Konrad
Published: May 2023

As non-unions are still common, a predictive assessment of healing complications could enable immediate intervention before negative impacts for the patient occur. The aim of this pilot study was to predict consolidation with the help of a numerical simulation model. A total of 32 simulations of patients with closed diaphyseal femoral shaft fractures treated by intramedullary nailing (PFNA long, FRN, LFN, and DePuy Synthes) were performed by creating 3D volume models based on biplanar postoperative radiographs. An established fracture healing model, which describes the changes in tissue distribution at the fracture site, was used to predict the individual healing process based on the surgical treatment performed and full weight bearing. The assumed consolidation as well as the bridging dates were retrospectively correlated with the clinical and radiological healing processes. The simulation correctly predicted 23 uncomplicated healing fractures. Three patients showed healing potential according to the simulation, but clinically turned out to be non-unions. Four out of six non-unions were correctly detected as non-unions by the simulation, and two simulations were wrongfully diagnosed as non-unions. Further adjustments of the simulation algorithm for human fracture healing and a larger cohort are necessary. However, these first results show a promising approach towards an individualized prognosis of fracture healing based on biomechanical factors.
Progress and Challenges of Immune Checkpoint Inhibitor-Induced Hypophysitis
Authors: Chen Piaohong , Li Jianwei , Tan Huiwen
Published: May 2023

Immune checkpoint inhibitors (ICIs) are a new type of antitumor drug which can achieve antitumor goals by blocking the binding of immune checkpoints to their ligands, thereby enhancing the activity of T cells. Meanwhile, ICIs block the binding of immune checkpoints to their ligands, disrupting the immune tolerance of T cells to self-antigens, which may lead to a series of immune-related adverse events (irAEs). Immune checkpoint inhibitor-induced hypophysitis (IH) is a relatively rare irAE. Due to the lack of specificity in clinical manifestations, it is difficult to accurately diagnose IH in a timely manner in clinical practice. However, the risk of adverse events, especially IH, for patients receiving ICIs has not been adequately investigated. Missed or delayed diagnosis may lead to poor prognosis or even adverse clinical outcomes. In this article, we summarize the epidemiology, pathogenesis, clinical manifestations, diagnosis and treatment of IH.
Fabrication of Biomedical Ti-Zr-Nb by Reducing Metal Oxides with Calcium Hydride
Authors: Yudin Sergey , Alimov Ivan , Volodko Sergey , Gurianov Alexander , Markova Galina , Kasimtsev Anatoly , Sviridova Tatiana , Permyakova Darya , Evstratov Evgeny , Cheverikin Vladimir , Moskovskikh Dmitry
Published: May 2023

In the present study, a powder of Ti-18Zr-15Nb biomedical alloy with spongy morphology and with more than 95% vol. of β-Ti was obtained by reducing the constituent oxides with calcium hydride. The influence of the synthesis temperature, the exposure time, and the density of the charge (TiO2 + ZrO2 + Nb2O5 + CaH2) on the mechanism and kinetics of the calcium hydride synthesis of the Ti-18Zr-15Nb β-alloy was studied. Temperature and exposure time were established as crucial parameters with the help of regression analysis. Moreover, the correlation between the homogeneity of the powder obtained and the lattice microstrain of β-Ti is shown. As a result, temperatures above 1200 °C and an exposure time longer than 12 h are required to obtain a Ti-18Zr-15Nb powder with a single β-phase structure and uniformly distributed elements. The analysis of β-phase growth kinetics revealed that the formation of β-Ti occurs due to the solid-state diffusion interaction between Ti, Nb, and Zr under the calcium hydride reduction of TiO2 + ZrO2 + Nb2O5, and the spongy morphology of reduced α-Ti is inherited by the β-phase. Thus, the results obtained provide a promising approach for manufacturing biocompatible porous implants from β-Ti alloys that are believed to be attractive candidates for biomedical applications. Moreover, the current study develops and deepens the theory and practical aspects of the metallothermic synthesis of metallic materials and can be compelling to specialists in powder metallurgy.
Cellular Alterations in Carbohydrate and Lipid Metabolism Due to Interactions with Nanomaterials
Authors: Martín-Pardillos Ana , Martin-Duque Pilar
Published: May 2023

Nanoparticles (NPs) have unique physicochemical properties that are useful for a broad range of biomedical and industrial applications; nevertheless, increasing concern exists about their biosafety. This review aims to focus on the implications of nanoparticles in cellular metabolism and their outcomes. In particular, some NPs have the ability to modify glucose and lipid metabolism, and this feature is especially interesting to treat diabetes and obesity and to target cancer cells. However, the lack of specificity to reach target cells and the toxicological evaluation of nontargeted cells can potentially induce detrimental side effects, closely related to inflammation and oxidative stress. Therefore, identifying the metabolic alterations caused by NPs, independent of their application, is highly needed. To our knowledge, this increase would lead to the improvement and safer use with a reduced toxicity, increasing the number of available NPs for diagnosis and treatment of human diseases.
Physical Characteristics and Biocompatibility of 3D-Printed Polylactic-Co-Glycolic Acid Membranes Used for Guided Bone Regeneration
Authors: Petposri Sidabhat , Thuaksuban Nuttawut , Buranadham Supanee , Suwanrat Trin , Punyodom Winita , Supphaprasitt Woraporn
Published: May 2023

Bioresorbable polymeric membranes for guided bone regeneration (GBR) were fabricated using the three-dimensional printing technique. Membranes made of polylactic-co-glycolic acid (PLGA), which consist of lactic acid (LA) and glycolic acid in ratios of 10:90 (group A) and 70:30 (group B), were compared. Their physical characteristics including architecture, surface wettability, mechanical properties, and degradability were compared in vitro, and their biocompatibilities were compared in vitro and in vivo. The results demonstrated that the membranes of group B had mechanical strength and could support the proliferation of fibroblasts and osteoblasts significantly better than those of group A (p < 0.05). The degradation rate in Group B was significantly lower than that in Group A, but they significantly produced less acidic environment (p < 0.05). In vivo, the membranes of group B were compared with the commercially available collagen membranes (group C). The amount of newly formed bone of rat’s calvarial defects covered with the membranes of group C was stable after week 2, whereas that of group B increased over time. At week 8, the new bone volumes in group B were greater than those in group C (p > 0.05). In conclusion, the physical and biological properties of the PLGA membrane (LA:GA, 70:30) were suitable for GBR.
Leader’s Possession of Linguistic Intelligence in Relation to Leader–Member Exchange Theory
Authors: Ribič Timotej , Marič Miha
Published: May 2023

When practicing high-quality leader–member exchange (LMX) theory, the leader’s ability to communicate, build and maintain relationships is a vital part. Because leader–member exchange theory is a relationship-based approach to leadership that primarily includes social exchange and communication on a daily basis, we can highlight linguistic intelligence as a key leadership skill that is part of the multiple intelligences defined by Howard Gardner. The goal of this article was to conduct research into organizations where the leader applies LMX theory and examine whether the leader’s linguistic intelligence is positively related to the quality of the leader–member exchange. The dependent variable was the quality of the LMX. We were able to recruit 39 employees and 13 leaders. Correlations and multiple regressions were used to analyze our statement. The overall results are statistically significant and we conclude that there is a high positive correlation between LMX and linguistic intelligence in the organizations that were part of this study. A limitation of this study is the use of purposive sampling, which resulted in a relatively small sample size and may limit the generalization of the results to other populations.
Steady-State Motion of a Load on an Ice Cover with Linearly Variable Thickness in a Channel
Authors: Shishmarev Konstantin , Khabakhpasheva Tatyana , Oglezneva Kristina
Published: May 2023

The paper considers the visco-elastic response of the ice cover in a channel under an external load moving with constant speed along the center line. The channel has a rectangular cross-section with a finite depth and width. The fluid in the channel is inviscid and incompressible and its motion is potential. The fluid is covered by a thin sheet of ice frozen to the channel walls. The ice thickness varies linearly symmetrically across the channel, being lowest at the center of the channel and highest at the channel walls. Ice deflections and strains in the ice cover are independent of time in the coordinate system moving with the load. The problem is solved numerically using Fourier transform along the channel and the method of normal modes across the channel. The series coefficients for normal modes are determined by truncation for the resulting infinite systems of linear algebraic equations. The ice deflections and strains in the ice plate are investigated and compared to the case of constant mean ice thickness. It is shown that even a small variation of the ice thickness significantly changes the characteristics of the hydroelastic waves in the channel.
Scenario Analysis of Cost-Effectiveness of Maintenance Strategies for Fixed Tidal Stream Turbines in the Atlantic Ocean
Authors: Kamidelivand Mitra , Deeney Peter , McAuliffe Fiona Devoy , Leyne Kevin , Togneri Michael , Murphy Jimmy
Published: May 2023

This paper has developed an operation and maintenance (O&M) model for projected 20 MW tidal stream farm case studies at two sites in the northeast Atlantic in France and at EMEC’s Fall of Warness site in the UK. The annual energy production, number of incidents, and downtimes of the farms for corrective and planned (preventive) maintenance strategies are estimated using Monte Carlo simulations that vary weather windows, repair vessel availabilities, and mean annual failure rates modelled by Weibull distributions. The trade-offs between the mean annual failure rates, time availability, O&M costs, and energy income minus the variable O&M costs were analysed. For all scenarios, a 5-year planned maintenance strategy could considerably decrease the mean annual failure rates by 37% at both sites and increase the net energy income. Based on a detailed sensitivity analysis, the study has suggested a simple decision-making method that examines how the variation in the mean annual failure rate and changes in spare-part costs would reduce the effectiveness of a preventive maintenance strategy. This work provides insights into the most important parameters that affect the O&M cost of tidal stream turbines and their effect on tidal energy management. The output of the study will contribute to decision-making concerning maintenance strategies.