bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of the Dietary Intake of Vitamin B12, Folic Acid, and Vitamin D3 on Homocysteine Levels and the Health-Related Quality of Life of Levodopa-Treated Patients with Parkinson’s Disease—A Pilot Study in Romania
Authors: Turcu-Stiolica Adina , Naidin Mihaela-Simona , Halmagean Steliana , Ionescu Ana , Pirici Ionica
Published: July 2024

Background and Objectives: Previous studies have shown that the levodopa treatment of Parkinson’s disease (PD) elevates circulating homocysteine levels, which are associated with an increased risk of cardiovascular and neurological disorders, or thrombosis. The present trial aimed to examine whether the intake of vitamin B12, folic acid, and vitamin D3 supplements improved homocysteine level and quality of life (QoL). Materials and Methods: An interventional prospective trial was conducted in multiple centers across Romania. Participants with clinically established PD taking at least 300 mg/day of levodopa for more than 1 year received a daily tablet of a supplement containing 800 UI of vitamin D3, 1000 µg of folic acid, and 15 µg of vitamin B12. They were followed for 6 months and their serum homocysteine, vitamin B12, vitamin D, and QoL scores were measured at baseline and at 6 months of treatment. QoL was measured using a 15D questionnaire, which assesses mobility, vision, hearing, breathing, sleeping, eating, speech, excretion, usual activities, mental function, discomfort and symptoms, depression, distress, vitality, and sexual activity. Results: Twenty-four PD patients with a mean age of 71 ± 5.04 years (54.2% male and 45.8% female) finished the study. After the intervention, the mean score of speech, mental function, discomfort and symptoms, depression, and QoL significantly increased (p < 0.05 for all). Also, the serum homocysteine and vitamin D were significantly enhanced (p < 0.0001 and p = 0.025, respectively). Changes in vitamin B12 were not statistically significant at 6 months of treatment (p = 0.996). No gender differences were found among the changes that we have demonstrated for homocysteine, vitamin B12, vitamin D, and QoL levels (p < 0.05 for all). Conclusions: The findings of this study showed that the dietary intake of vitamin B12, folic acid, and vitamin D3 remarkably decreased the dimensions of homocysteine and finally increased the total score of QoL in PD patients. We have successfully captured the potential benefits of the supplementation regimen over time and provided insights into the broader implications for managing PD with a focus on nutritional support.
Early Diagnosis and Treatment of Mandibular Second Premolar Impaction: A Case Report
Authors: Janosy Anna-Maria , Moca Abel Emanuel , Juncar Raluca Iulia
Published: July 2024

Odontogenesis, the process of tooth formation, is complex and susceptible to disruptions that can result in dental anomalies such as tooth impaction. The mandibular second premolar, though less commonly impacted than wisdom teeth, presents a unique challenge in pediatric dentistry due to its intricate etiology and the need for timely intervention. This case report aims to highlight the significance of early diagnosis and conservative management strategies in treating mandibular second premolar impaction. The case involves a pediatric patient with impacted mandibular second premolars. Initial treatment included the use of a lower removable appliance with an expansion screw to alleviate crowding, followed by a fixed space maintainer and a Haas rapid palatal expander. These interventions created the necessary space for the premolars to erupt. Self-ligating brackets were later applied, reducing friction and improving periodontal health. The patient underwent two CBCT examinations to monitor progress, which confirmed the successful eruption and alignment of the impacted premolars without the need for surgical exposure. This case underscores the effectiveness of early diagnosis and minimally invasive treatment in managing mandibular second premolar impaction. The tailored approach facilitated the natural eruption of the teeth, highlighting the importance of individualized treatment plans. Future research should focus on optimizing these conservative strategies to enhance patient outcomes in similar cases.
Comparison of the Direct Identification and Short-Term Incubation Methods for Positive Blood Cultures via MALDI-TOF Mass Spectrometry
Authors: Kuo Shu-Fang , Huang Tsung-Yu , Lee Chih-Yi , Lee Chen-Hsiang
Published: July 2024

Timely pathogen identification in bloodstream infections is crucial for patient care. A comparison is made between positive blood culture (BC) pellets from serum separator tubes using a direct identification (DI) method and colonies on agar plates from a short-term incubation (STI) method with a matrix-assisted laser desorption/ionization Biotyper for the evaluation of 354 monomicrobial BCs. Both the DI and STI methods exhibited similar identification rates for different types of bacteria, except for Gram-positive and anaerobic bacteria. The DI method’s results aligned closely with the STI method’s results for Enterobacterales, glucose-non-fermenting Gram-negative bacilli (GNB), and carbapenem-resistant Enterobacterales. The DI method exhibited high concordance with the conventional method for GNB identification, achieving 88.2 and 87.5% accuracy at the genus and species levels, respectively. Compared with the STI method, the DI method showed a less successful performance for Gram-positive bacterial identification (50.5 vs. 71.3%; p < 0.01). The DI method was useful for anaerobic bacterial identification of slow-growing microorganisms without any need for colony growth, unlike in the STI method (46.7 vs. 13.3%; p = 0.04). However, both methods could not identify yeast in positive BCs. Overall, the DI method provided reliable results for GNB identification, offering many advantages over the STI method by significantly reducing the turnaround time and enabling quicker pathogen identification in positive BCs.
The INVISION Talar Component in Revision Total Ankle Arthroplasty: Analysis of Early Outcomes
Authors: Valan Bruno , Anastasio Albert T. , Kim Billy , Krez Alexandra , Wu Kevin A. , Talaski Grayson M. , Nunley James , DeOrio James K. , Easley Mark E. , Adams Samuel B.
Published: July 2024

Introduction: Launched in 2018 for revision total ankle arthroplasty (rTAA), the INVISION talar component addresses subsidence when poor talar bone stock is present. Due to the recency of the market-availability of the INVISION, studies evaluating its efficacy are lacking. This study presents the first analysis of early-term outcomes of patients undergoing rTAA with the INVISION talar component. Methods: This was a single-center, retrospective review of 28 patients undergoing rTAA with the INVISION talar component and INBONE II tibial component performed between 2018 and 2022. Data on preoperative characteristics, postoperative complications, secondary procedures, and survivorship were collected. The primary outcome measures were rates of major complications, re-operation, and implant failure. Secondary outcomes included post-operative changes in varus and valgus alignment of the tibia and talus. Results: The most common secondary procedures performed with rTAA were medial malleolus fixation (n = 22, 78.6%) and gastrocnemius recession (n = 14, 50%). Overall, 10.7% (n = 3) of patients underwent reoperation and 14.3% (n = 4) suffered major complications. Incidence of implant failure was 10.7% (n = 3). All reoperations were caused by infection. Mean varus alignment of the tibia and talus improved from 4.07 degrees and 4.83 degrees to 1.67 degrees and 1.23 degrees, respectively. Mean valgus alignment of the tibia and talus improved from 3.67 degrees and 4.22 degrees to 2.00 degrees and 2.32 degrees, respectively. Conclusions: In a series of 28 patients undergoing rTAA with the INVISION talar component, we discovered comparatively low rates of reoperation, major complication, and implant failure (10.7%, 14.3%, and 10.7%). The INVISION system appears to have a reasonable safety profile, but further studies evaluating long-term outcomes are required to assess the efficacy of the INVISION system.
Confocal Microscopy of the Cornea in Aqueous-Deficient Dry Eye Disease—A Literature Review
Authors: Bucsan Radu , Coroleucă Ruxandra , Garhöfer Gerhard , Popa-Cherecheanu Alina , Schmetterer Leopold , Iancu Raluca
Published: July 2024

Background: In vivo confocal microscopy (IVCM) is a vital tool in studying dry eye disease (DED), providing insights into morphological changes at ocular surface unit levels. This review presents the main differences in corneal structure between aqueous-deficient dry eye disease (AD-DED) and normal eyes. Methods: A comprehensive search of PubMed, Web of Science, Embase, and MEDLINE databases from January 2000 to December 2023 was conducted. The study selection process, as well as data selection and examination, were independently performed by two members of the review team. Results: The review reveals a consistent decrease in corneal surface epithelial cell density in AD-DED cases compared to a control group, but conflicting data on basal epithelial cell density. Notably, the abnormal hyperreflectivity of keratocytes in patients with Sjogren’s syndrome was recorded, and there was a significant keratocyte density in AD-DED subjects compared to evaporative DED and control groups. Studies also found a decrease in sub-basal nerve density, increased tortuosity, and the fragmentation of nerve fibers. Dendritic cell density and dendritic cell dendrites increase in AD-DED patients compared to healthy subjects. Conclusions: IVCM is a powerful tool for enhancing our understanding of the pathophysiological mechanisms underlying DED. However, the review underscores the urgent need to standardize the terminology, analysis, and units used for accurate interpretation, a crucial step in advancing our knowledge of DED.
Localised Objective Characterisation Assessment of Lymphoedema (LOCAL): Using High-Frequency Ultrasound, Bioelectrical Impedance Spectroscopy and Volume to Evaluate Superficial Tissue Composition
Authors: Sanderson Jennifer , Tuttle Neil , Box Robyn , Reul-Hirche Hildegard , Laakso E-Liisa
Published: July 2024

Lymphoedema tissue is characterised by excess free fluid and structural changes to the extracellular matrix (ECM) in the form of fibrotic and fatty deposition. These tissue characteristics are integral to the assessment of lymphoedema progression; however, clinicians and researchers often focus on changes in the free fluid, volume and function of lymphatic vasculature to inform practice. Subsequently, little is known about the effect of clinical interventions on lymphoedema tissue composition. This article presents a novel approach to classify lymphoedema tissue. The Localised Objective Characterisation Assessment of Lymphoedema (LOCAL) classification combines diagnostic and clinically meaningful objective assessment thresholds to infer lymphoedema pathophysiological changes in tissue layers. The LOCAL classification method was verified using data from fifteen women with unilateral breast cancer-related lymphoedema who were evaluated at three sites on each arm using high-frequency ultrasound (HFUS), bio-electrical impedance spectroscopy (BIS) and volume measurements. Participants exhibited an uneven distribution of volume between the proximal and distal segments of the arm (p = 0.023), with multiple tissue compositional categories observed across sites on the same limb (p < 0.001). The LOCAL method demonstrated utility in categorising a diverse range of lymphoedema tissue layer changes beyond what can be ascertained from whole-limb measures.
Development of Multi-Motor Servo Control System Based on Heterogeneous Embedded Platforms
Authors: Gou Mingrui , Wang Bangji , Zhang Xilin
Published: July 2024

Multi-motor servo systems are widely used in industrial control. However, the single-core microprocessor architecture based on the microcontroller unit (MCU) and digital signal processor (DSP) is not well suited for high-performance multi-motor servo systems due to the inherent limitations in computing performance and serial execution of code. The bus-based distributed architecture formed by interconnecting multiple unit controllers increases system communication complexity, reduces system integration, and incurs additional hardware and software costs. Field programmable gate array (FPGA) possesses the characteristics of high real-time performance, parallel processing, and modularity. A single FPGA can integrate multiple motor servo controllers. This research uses MCU + FPGA as the core to realize high-precision multi-axis real-time control, combining the powerful performance of the MCU processor and the high-speed parallelism of FPGA. The MCU serves as the central processor and facilitates data interaction with the host computer through the controller area network (CAN). After data parsing and efficient computation, MCU communicates with the FPGA through flexible static memory controller (FSMC). A motor servo controller intellectual property (IP) core is designed and packaged for easy reuse within the FPGA. A 38-axis micro direct current (DC) motor control system is constructed to test the performance of the IP core and the heterogeneous embedded platforms. The experimental results show that the designed IP core exhibits robust functionality and scalability. The system exhibits high real-time performance and reliability.
Synthesis of Non-Uniform Spiral Antenna with Low Peak Sidelobe Level Using Enhanced Harris Hawks Optimization Algorithm
Authors: Li Tianlong , Liu Zhijun , Zhang Chen , Cheng Fei , Yao Yali , Li Xiumei , He Haidan , Yang Yang
Published: July 2024

In this paper, to obtain antenna arrays with grating lobes suppression capability in wideband and achieve a low peak sidelobe level (PSLL), two non-uniform spiral antenna arrays and an enhanced Harris Hawks optimization (EHHO) algorithm are proposed. By controlling the parameters of the spiral line and sampling equidistant on the spiral line, the sampling points that make up the non-uniform array can be arranged in the plane uniformly and non-uniformly. The simulation results indicate that, because of this special arrangement, the non-uniform arrays obtain the capability of grating lobe suppression in wideband or wide spacing arrangement when compared to the classic uniform array. In addition, to obtain lower PSLL, the Harris Hawks optimization (HHO) algorithm is used for array synthesis because of its diversity of search methods. By employing the step-type taper distribution strategy and the migration strategy, the algorithm’s search ability is enhanced, and the simulation results indicate the EHHO algorithm obtains a better solution in terms of the PSLL than other algorithms. A simple patch antenna is designed to build the non-uniform spiral arrays synthesized by the EHHO algorithm. The calculation and simulation results validate the superior performance of the proposed algorithm.
A Review of Post-Quantum Privacy Preservation for IoMT Using Blockchain
Authors: Sabrina Fariza , Sohail Shaleeza , Tariq Umair Ullah
Published: July 2024

The Internet of Medical Things (IoMT) has significantly enhanced the healthcare system by enabling advanced patient monitoring, data analytics, and remote interactions. Given that IoMT devices generate vast amounts of sensitive data, robust privacy mechanisms are essential. This privacy requirement is critical for IoMT as, generally, these devices are very resource-constrained with limited storage, computation, and communication capabilities. Blockchain technology, with its decentralisation, transparency, and immutability, offers a promising solution for improving IoMT data security and privacy. However, the recent emergence of quantum computing necessitates developing measures to maintain the security and integrity of these data against emerging quantum threats. This work addresses the current gap of a comprehensive review and analysis of the research efforts to secure IoMT data using blockchain in the quantum era. We discuss the importance of blockchain for IoMT privacy and analyse the impact of quantum computing on blockchain to justify the need for these works. We also provide a comprehensive review of the existing literature on quantum-resistant techniques for effective blockchain solutions in IoMT applications. From our detailed review, we present challenges and future opportunities for blockchain technology in this domain.
Multi-Utility Solar Thermal Systems: Harnessing Parabolic Trough Concentrator Using SAM Software for Diverse Industrial and Residential Applications
Authors: Naaim Soufyane , Ouhammou Badr , Aggour Mohammed , Daouchi Brahim , El Mers El Mahdi , Mihi Miriam
Published: July 2024

This study investigates the technical and economic feasibility of a 20 MW parabolic trough solar thermal power plant (PTSTPP) located in Kenitra, Morocco, characterized by an annual average direct normal irradiance (DNI) exceeding 5.3 kWh/m2/day. Utilizing System Advisor Model (SAM) 2012.12.02 software, the plant is designed with Therminol VP-1 as the heat transfer fluid (HTF) throughout the solar field, coupled with a dry cooling system to reduce water consumption. The proposed thermal energy storage (TES) system employs HITEC solar salt as the storage medium, allowing for six full load hours of thermal energy storage. With a solar multiple (SM) of 2, the simulated plant demonstrates the capability to generate an annual electricity output of 50.51 GWh. The economic viability of the plant is further assessed, revealing a Levelized Cost of Electricity (LCOE) of 0.1717 $/kWh and a capacity factor (CF) of 32%. This comprehensive analysis provides valuable insights into the performance, economic viability, and sustainability of a parabolic trough solar power plant in the specific climatic conditions of Kenitra, Morocco.
The Expanding Thread of Ungulate Browsing—A Review of Forest Ecosystem Effects and Management Approaches in Europe
Authors: Hardalau Darius , Codrean Codrin , Iordache Daniel , Fedorca Mihai , Ionescu Ovidiu
Published: July 2024

In recent decades, ungulates have expanded in number and range in Europe. This review aims to analyze the impact of ungulate browsing in different forest ecosystems and identify the main driving factors and trends. In total, 155 studies were analyzed in preparing this review, across 19 European countries. In Europe, the main browsers are represented by roe deer (Capreolus capreolus L.), red deer (Cervus elaphus L.), moose (Alces alces L.), chamois (Rupicapra rupicapra L.), and fallow deer (Dama dama L.). Regarding browsing severity, they frequently exceeded 50%, meaning that over half of the saplings were browsed. Ungulate density was the main driving factor of browsing severity, with areas exhibiting high browsing pressure often having more than ten individuals per square kilometer. The type of silvicultural system used played a vital role in the severity of browsing, and trends in foraging for preferred tree species were identified. Fencing was the most common non-harmful protection method used, while hunting management was the most efficient method for controlling deer numbers and browsing intensity. Large carnivores were missing in most study areas, but in the areas where they were present, they played a significant role in creating a chain reaction of ecological impacts. Considering the significant impact of ungulate browsing on forest ecosystems, there is a pressing need for more research to comprehend and effectively mitigate the effects of deer presence comprehensively.
Impact and Spatial Effect of Government Environmental Policy on Forestry Eco-Efficiency—Examining China’s National Ecological Civilization Pilot Zone Policy
Authors: Yan Yingzheng , Zhou Ziwei , Chen Liupeng , Wei Yuanzhu
Published: July 2024

Can government environmental policy harmonize environmental protection with economic output? We explore this issue from the perspective of forestry eco-efficiency, using China’s National Ecological Civilization Pilot Zone Policy (NECP), an environmental policy promulgated by the government of China, as the subject of this study. The study introduces forestry eco-efficiency as an indicator to assess the balance between economic development in the forestry sector and environmental conservation. The indicator, grounded in sustainable development theory, employs a super-efficiency SBM model that includes undesirable outputs to evaluate efficiency. Additionally, we empirically analyze the impact of NECP on forestry eco-efficiency by using the difference-in-difference (DID) model with provincial panel data from 2011 to 2020. Ultimately, we analyze the effects of spatial spillover by employing the spatial Durbin model (SDM). Our study yields the following conclusions. (1) In this paper, through hotspot clustering analysis, forestry eco-efficiency in each province is categorized into three categories: effective, semi-effective and ineffective. Our findings suggest that China’s average forestry eco-efficiency falls into the ineffective category, highlighting the need to optimize resource allocation within the sector. (2) NECP significantly enhances forestry eco-efficiency, with robust findings across various stability tests. Thus, implementing government environmental policies can have a multiplier effect on forestry, i.e., it can synergize its economic development with environmental protection. (3) In provinces with a strong ecological foundation, the NECP significantly enhances forestry eco-efficiency. However, in other provinces, the improvement is only moderate. Furthermore, while the NECP has a substantial positive impact in the eastern region, it has yet to show a discernible effect in other regions. (4) The positive impacts of NECP implementation on forestry eco-efficiency have spatial spillover effects due to demonstration effects and comparative advantages.
The High-Molecular-Weight Glutenin Subunits of the T. timopheevii (AuAuGG) Group
Authors: Margiotta Benedetta , Colaprico Giuseppe , Urbano Marcella , Panichi Daniela , Sestili Francesco , Lafiandra Domenico
Published: July 2024

Polyploid wheats include a group of tetraploids known as Timopheevii (AuAuGG), which are represented by two subspecies: Triticum timopheevii ssp. timopheevii (cultivated) and Triticum timopheevii ssp. araraticum (wild). The combined use of electrophoretic (SDS-PAGE) and chromatographic (RP-HPLC) techniques carried out on high-molecular-weight glutenin subunits (HMW-GSs) permitted the association of different x- and y-type subunits to the A and G genomes and the assessment of allelic variation present at corresponding loci. The results also revealed that in both subspecies, accessions are present that possess expressed y-type subunits at the Glu-A1 locus. Genes corresponding to these subunits were amplified and amplicons corresponding to x- and y-type genes associated with the A genome were detected in all accessions, including those without expressed x- and y-type subunits. The comparison with genes of polyploid wheats confirmed the structural characteristics of typical y-type genes, with the presence of seven cysteine residues and with hexapeptide and nonapeptide repeat motifs. The identification of wild and cultivated T. timopheevii with both x- and y-type glutenin subunits at the Glu-A1 and Glu-G1 loci represents a useful source for the modification of the allelic composition of HMW-GSs in cultivated wheats with the ultimate objective of improving technological properties.
Analysis of Debris Flow Protective Barriers Using the Coupled Eulerian Lagrangian Method
Authors: Sha Shiyin , Dyson Ashley P. , Kefayati Gholamreza , Tolooiyan Ali
Published: July 2024

Protective structures play a vital role in mitigating the risks associated with debris flows, yet assessing their performance poses crucial challenges for their real-world effectiveness. This study proposes a comprehensive procedure for evaluating the performance of protective structures exposed to impacts from media transported by large debris flow events. The method combines numerical modelling with site conditions for existing structures along the Hobart Rivulet in Tasmania, Australia. The Coupled Eulerian Lagrangian (CEL) model was validated by comparing simulation results with experimental data, demonstrating high agreement. Utilising three-dimensional modelling of debris flow–boulder interactions over the Hobart Rivulet terrain, boulder velocities were estimated for subsequent finite element analyses. Importantly, a model of interaction between boulders and I-beam posts was established, facilitating a comparative assessment of five distinct I-beam barrier systems defined as Type A to E, which are currently in use at the site. Simulation results reveal larger boulders display a slower increase in their velocities over the 3D terrain. Introducing a key metric, the failure ratio, enable a mechanism for comparative assessments of these barrier systems. Notably, the Type E barriers demonstrate superior performance due to fewer weak points within the structure. The combined CEL and FE assessments allow for multiple aspects of the interactions between debris flows, boulders, and structures to be considered, including structural failure and deformability, to enhance the understanding of debris flow risk mitigation in Tasmania.
The Perspective of Using Neural Networks and Machine Learning Algorithms for Modelling and Forecasting the Quality Parameters of Coking Coal—A Case Study
Authors: Dyczko Artur
Published: July 2024

The quality of coking coal is vital in steelmaking, impacting final product quality and process efficiency. Conventional forecasting methods often rely on empirical models and expert judgment, which may lack accuracy and scalability. Previous research has explored various methods for forecasting coking coal quality parameters, yet these conventional methods frequently fall short in terms of accuracy and adaptability to different mining conditions. Existing forecasting techniques for coking coal quality are limited in their precision and scalability, necessitating the development of more accurate and efficient methods. This study aims to enhance the accuracy and efficiency of forecasting coking coal quality parameters by employing neural networks and artificial intelligence algorithms, specifically in the context of Knurow and Szczyglowice mines. The research involves gathering historical data on various coking coal quality parameters, including a proximate and ultimate analysis, to train and test neural network models using the Group Method of Data Handling (GMDH). Real-world data from Knurow and Szczyglowice mines’ coal production facilities form the basis of this case study. The integration of neural networks and artificial intelligence techniques significantly improves the accuracy of predicting key quality parameters such as ash content, sulfur content, volatile matter, and calorific value. This study also examines the impact of these quality indicators on operational costs and highlights the importance of final indicators like the Coke Reactivity Index (CRI) and Coke Strength after Reaction (CSR) in expanding industrial reserve concepts. Model performance is evaluated using metrics such as mean absolute error (MAE), root mean square error (RMSE), and coefficient of determination (R2). The findings demonstrate the effectiveness of these advanced techniques in enhancing predictive modeling in the mining industry, optimizing production processes, and improving overall operational efficiency. Additionally, this research offers insights into the practical implementation of advanced analytics tools for predictive maintenance and decision-making support within the mining sector.
Raising Awareness about Sex Trafficking among School Personnel
Authors: Savoia Elena , Liu Amy , Leffler Amy , Nadril Churchill Léa Kay , Su Maxwell
Published: July 2024

Background: We aimed to (1) understand the level of knowledge about sex trafficking of minors among school personnel and the determinants of such knowledge and (2) test the efficacy of short educational videos in increasing knowledge (awareness level) about sex trafficking of minors among school personnel. Methods: We employed an online survey to gather responses from 741 school personnel living in the US. The McNemar test was used to test for differences in knowledge before and after exposure to the videos. Logistic regression was used to identify predictors of knowledge based on the respondents’ characteristics. Results: Predictors of knowledge about sex trafficking were years of experience in working with youth, level of education, and being a female. Exposure to the educational videos improved school personnel’s basic knowledge about this crime and interest in seeking additional educational material. Conclusion: School personnel have a high level of awareness of risk factors for sex trafficking but less awareness of the definition of sex trafficking in children. Exposure to short educational videos can increase awareness in the short term. There is a need to develop more comprehensive training initiatives for school personnel on sex trafficking. However, training alone is not sufficient, and there is also a need for developing school protocols and programs to provide adequate support to victims of this crime.
Automatic Vehicle Trajectory Behavior Classification Based on Unmanned Aerial Vehicle-Derived Trajectories Using Machine Learning Techniques
Authors: Teo Tee-Ann , Chang Min-Jhen , Wen Tsung-Han
Published: July 2024

This study introduces an innovative scheme for classifying uncrewed aerial vehicle (UAV)-derived vehicle trajectory behaviors by employing machine learning (ML) techniques to transform original trajectories into various sequences: space–time, speed–time, and azimuth–time. These transformed sequences were subjected to normalization for uniform data analysis, facilitating the classification of trajectories into six distinct categories through the application of three ML classifiers: random forest, time series forest (TSF), and canonical time series characteristics. Testing was performed across three different intersections to reveal an accuracy exceeding 90%, underlining the superior performance of integrating azimuth–time and speed–time sequences over conventional space–time sequences for analyzing trajectory behaviors. This research highlights the TSF classifier’s robustness when incorporating speed data, demonstrating its efficiency in feature extraction and reliability in intricate trajectory pattern handling. This study’s results indicate that integrating direction and speed information significantly enhances predictive accuracy and model robustness. This comprehensive approach, which leverages UAV-derived trajectories and advanced ML techniques, represents a significant step forward in understanding vehicle trajectory behaviors, aligning with the goals of enhancing traffic control and management strategies for better urban mobility.
Advanced Vibrational Spectroscopy and Bacteriophages Team Up: Dynamic Synergy for Medical and Environmental Applications
Authors: Giergiel Magdalena , Chakkumpulakkal Puthan Veettil Thulya , Rossetti Ava , Kochan Kamila
Published: July 2024

Bacteriophages are emerging as a promising alternative in combating antibiotic-resistant bacteria amidst the escalating global antimicrobial resistance crisis. Recently, there has been a notable resurgence of interest in phages, prompting extensive research into their therapeutic potential. Beyond conventional microbiology and virology techniques, such as genomics and proteomics, novel phenotypic and chemical characterization methods are being explored. Among these, there is a growing interest in vibrational spectroscopy, especially in advanced modalities such as surface-enhanced Raman spectroscopy (SERS), tip-enhanced Raman spectroscopy (TERS), and atomic force microscopy-infrared spectroscopy (AFM-IR), which offer improved sensitivity and spatial resolution. This review explores the spectrum of uses of vibrational spectroscopy for bacteriophages, including its role in diagnostics, biosensing, phage detection, assistance in phage-based therapy, and advancing basic research.
Divergent Roles of the Auxin Response Factors in Lemongrass (Cymbopogon flexuosus (Nees ex Steud.) W. Watson) during Plant Growth
Authors: Wang Guoli , Zeng Jian , Du Canghao , Tang Qi , Hua Yuqing , Chen Mingjie , Yang Guangxiao , Tu Min , He Guangyuan , Li Yin , He Jinming , Chang Junli
Published: July 2024

Auxin Response Factors (ARFs) make up a plant-specific transcription factor family that mainly couples perception of the phytohormone, auxin, and gene expression programs and plays an important and multi-faceted role during plant growth and development. Lemongrass (Cymbopogon flexuosus) is a representative Cymbopogon species widely used in gardening, beverages, fragrances, traditional medicine, and heavy metal phytoremediation. Biomass yield is an important trait for several agro-economic purposes of lemongrass, such as landscaping, essential oil production, and phytoremediation. Therefore, we performed gene mining of CfARFs and identified 26 and 27 CfARF-encoding genes in each of the haplotype genomes of lemongrass, respectively. Phylogenetic and domain architecture analyses showed that CfARFs can be divided into four groups, among which groups 1, 2, and 3 correspond to activator, repressor, and ETTN-like ARFs, respectively. To identify the CfARFs that may play major roles during the growth of lemongrass plants, RNA-seq was performed on three tissues (leaf, stem, and root) and four developmental stages (3-leaf, 4-leaf, 5-leaf. and mature stages). The expression profiling of CfARFs identified several highly expressed activator and repressor CfARFs and three CfARFs (CfARF3, 18, and 35) with gradually increased levels during leaf growth. Haplotype-resolved transcriptome analysis revealed that biallelic expression dominance is frequent among CfARFs and contributes to their gene expression patterns. In addition, co-expression network analysis identified the modules enriched with CfARFs. By establishing orthologous relationships among CfARFs, sorghum ARFs, and maize ARFs, we showed that CfARFs were mainly expanded by whole-genome duplications, and that the duplicated CfARFs might have been divergent due to differential expression and variations in domains and motifs. Our work provides a detailed catalog of CfARFs in lemongrass, representing a first step toward characterizing CfARF functions, and may be useful in molecular breeding to enhance lemongrass plant growth.
Sequential Nucleophilic Aromatic Substitution Reactions of Activated Halogens
Authors: Plater M. John , Harrison William T. A.
Published: July 2024

Building blocks have been identified that can be functionalised by sequential nucleophilic aromatic substitution. Some examples are reported that involve the formation of cyclic benzodioxin and phenoxathiine derivatives from 4,5-difluoro-1,2-dinitrobenzene, racemic quinoxaline thioethers, and sulfones from 2,3-dichloroquinoxaline and (2-aminophenylethane)-2,5-dithiophenyl-4-nitrobenzene from 1-(2-aminophenylethane)-2-fluoro-4,5-dinitrobenzene. Four X-ray single-crystal structure determinations are reported, two of which show short intermolecular N–O…N “π hole” contacts.
Rare Germline Variants in the Adenomatous Polyposis Coli Gene Associated with Dental and Osseous Anomalies
Authors: Büki Gergely , Antal Gréta , Bene Judit
Published: July 2024

APC is a tumor suppressor gene that exerts its effect through the regulation of the Wnt signaling pathway. Loss of function mutations of the gene are associated with familial adenomatous polyposis (FAP). Early diagnosis in FAP patients is essential to prevent the development of colorectal cancer. Extraintestinal manifestations often precede the formation of the polyposis; therefore, these manifestations may serve as a clinical indicator for the condition. The aim of this study was to assess genotype–phenotype associations between the location of APC mutations and various extraintestinal features, mainly focusing on osseous and dental anomalies. Analyses of our cases and the mutations available in the literature with these manifestations revealed that mutations in the N-terminal region (amino acids 1–~1000) of the protein are more frequently associated with only osseous anomalies, whereas dental manifestations are more prevalent in mutations in the middle region (amino acids 1000–~2100). In addition, supernumerary teeth were found to be the most common dental feature. Since dental abnormalities often precede intestinal polyposis, dentists have a crucial role in the early identification of patients at risk.
Integrated Bioinformatics-Based Identification and Validation of Neuroinflammation-Related Hub Genes in Primary Open-Angle Glaucoma
Authors: Ullah Zakir , Tao Yuanyuan , Huang Jufang
Published: July 2024

Glaucoma is a leading cause of permanent blindness, affecting 80 million people worldwide. Recent studies have emphasized the importance of neuroinflammation in the early stages of glaucoma, involving immune and glial cells. To investigate this further, we used the GSE27276 dataset from the GEO (Gene Expression Omnibus) database and neuroinflammation genes from the GeneCards database to identify differentially expressed neuroinflammation-related genes associated with primary open-angle glaucoma (POAG). Subsequently, these genes were submitted to Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes for pathway enrichment analyses. Hub genes were picked out through protein-protein interaction networks and further validated using the external datasets (GSE13534 and GSE9944) and real-time PCR analysis. The gene–miRNA regulatory network, receiver operating characteristic (ROC) curve, genome-wide association study (GWAS), and regional expression analysis were performed to further validate the involvement of hub genes in glaucoma. A total of 179 differentially expressed genes were identified, comprising 60 upregulated and 119 downregulated genes. Among them, 18 differentially expressed neuroinflammation–related genes were found to overlap between the differentially expressed genes and neuroinflammation–related genes, with six genes (SERPINA3, LCN2, MMP3, S100A9, IL1RN, and HP) identified as potential hub genes. These genes were related to the IL-17 signaling pathway and tyrosine metabolism. The gene–miRNA regulatory network showed that these hub genes were regulated by 118 miRNAs. Notably, GWAS data analysis successfully identified significant single nucleotide polymorphisms (SNPs) corresponding to these six hub genes. ROC curve analysis indicated that our genes showed significant accuracy in POAG. The expression of these genes was further confirmed in microglia, Müller cells, astrocytes, and retinal ganglion cells in the Spectacle database. Moreover, three hub genes, SERPINA3, IL1R1, and LCN2, were validated as potential diagnostic biomarkers for high-risk glaucoma patients, showing increased expression in the OGD/R-induced glaucoma model. This study suggests that the identified hub genes may influence the development of POAG by regulation of neuroinflammation, and it may offer novel insights into the management of POAG.
Functional Characterization of an Odorant Receptor Expressed in Newly Hatched Larvae of Fall Armyworm Spodoptera frugiperda
Authors: Wang Zhiqiang , Wang Xiaoqing , Liu Weihao , Chen Run , Liu Yang
Published: July 2024

In the past decade, Spodoptera frugiperda has emerged as a significant invasive pest globally, posing a serious threat to agriculture due to its broad diet, migratory behavior, and ability to cause extensive plant damage. While extensive research has focused on the olfactory capabilities of adult S. frugiperda, understanding of the olfactory process in larvae remains limited, despite larvae playing a crucial role in crop damage. To address this gap, we identified an odorant receptor (OR), SfruOR40, expressed in the first-instar larvae through phylogenetic analysis. Using quantitative real-time PCR, we compared SfruOR40 expression levels in larvae and adults. We then characterized the function of SfruOR40 against 67 compounds using the Xenopus oocyte expression system and found that SfruOR40 responded to three plant volatiles. Further, behavioral experiments revealed a larval attraction to (−)-trans-Caryophyllene oxide. This study elucidates SfruOR40’s role in the olfactory recognition of newly hatched S. frugiperda larvae, expanding our knowledge of such mechanisms in Noctuid moths. Furthermore, it highlights the potential of plant-derived natural products for biological pest control from a behavioral ecology perspective.
First DNA Barcoding Survey in Bulgaria Unveiled Huge Diversity of Yeasts in Insects
Authors: Dimitrov Roumen , Gouliamova Dilnora , Guéorguiev Borislav , Smith Maudy , Groenewald Marizeth , Boekhout Teun
Published: July 2024

In this study, we conducted a comprehensive survey aimed at assessing the diversity of yeast species inhabiting the guts of various insect species collected mainly from two Bulgarian National Parks, namely, Rila, and Pirin. The insect specimens encompass a broad taxonomic spectrum, including representatives from Coleoptera, Orthoptera, Lepidoptera, Hymenoptera, Dermaptera, Isopoda, and Collembola. Yeast strains were identified with DNA barcoding using the ribosomal markers, specifically, the D1/D2 domains of the ribosomal large subunit (LSU) and the internal transcribed spacers regions ITS 1 + 2 (ITS). The analysis unveiled the presence of 89 ascomycetous and 18 basidiomycetous yeast isolates associated with the insect specimens. Furthermore, our study identified 18 hitherto unknown yeast species.
Comparative Study of Blood Neopterin and Biopterins in Patients with COVID-19 and Secondary Bacterial Infection
Authors: Eguchi Tomohiro , Niiyama Shuhei , Kamikokuryo Chinatsu , Madokoro Yutaro , Shimono Kenshin , Hara Satoshi , Ichinose Hiroshi , Kakihana Yasuyuki
Published: July 2024

Background/Objectives: As COVID-19 can be severe, early predictive markers of both severity and onset of secondary bacterial infections are needed. This study first examined changes over time in the levels of plasma neopterin (NP) and biopterins (BPs), among others, in patients with COVID-19 and then in those with secondary bacterial infection complications. Methods: Fifty-two patients with COVID-19 admitted to two tertiary care centers were included. They were divided into a severe group (intubated + mechanical ventilation) (n = 10) and a moderate group (non-intubated + oxygen administration) (n = 42), and changes over time in plasma NP, plasma BPs, IFN-γ, lymphocyte count, CRP, and IL-6 were investigated. Four of the patients in the severe group (n = 10) developed secondary bacterial infections during treatment. Plasma NP and plasma BPs of patients with bacterial sepsis (no viral infection) (n = 25) were also examined. Results: The plasma NP, IL-6, CRP, and SOFA levels were significantly higher in the severe group, while the IFN-γ level and lymphocyte count were significantly lower. The higher plasma NP in the severe group persisted only up to 1 week after symptom onset. The plasma BPs were higher in complications of bacterial infection. Conclusions: The timing of sample collection is important for assessing severity through plasma NP, while plasma BPs may be a useful diagnostic tool for identifying the development of secondary bacterial infection in patients with COVID-19. Further investigation is needed to clarify the mechanism by which NP and BPs, which are involved in the same biosynthetic pathway, are differentially activated depending on the type of pathogen.
Anterior Spinal Fusion for Thoraco-Lumbar Idiopathic Scoliosis Comparing Less Invasive Concave versus Traditional Convex Approach: A Pilot Study
Authors: Buttermann Glenn
Published: July 2024

Background/Objectives: Anterior spinal fusion for primary thoracolumbar or lumbar (TL/L) adolescent idiopathic scoliosis, AIS, has advantages over posterior fusion, particularly in saving motion segments below the fusion construct. Traditionally, the approach is anterolaterally from the convexity. In adult degenerative scoliosis, the lateral or anterolateral approach may be performed from the traditional or from the concave approach which is less invasive and gives comparable outcomes. The purpose of the present pilot study was to assess the feasibility of the less invasive concave approach for younger AIS patients and compare it to the traditional convex approach over a 5-year follow-up period. Methods: The two cohorts were assessed by comparing pre- to postoperative radiographs, and clinical outcomes for pain, function, self-perception of appearance, and opinion of surgical success were prospectively obtained. Results: Radiographs found that primary TL/L scoliosis significantly improved from 53° to 18° (65%) for both the concave and convex cohorts. Sagittal alignments remained stable and there was no difference between cohorts. Coronal balance improved in both cohorts and sagittal balance was stable for both. Clinically, VAS back pain improved significantly for both cohorts initially and remained improved in the concave group. Leg pain, pain drawing, ODI disability, and VAS appearance scores improved and there was no difference between cohorts. The self-rating of success of the procedure was 100% at early and late follow-up periods. There were no neurological/surgical complications. Conclusions: The concave approach for anterior fusion for TL/L AIS is feasible with comparable radiographic and clinical outcomes to the traditional approach.
Impact of Serum Indoxyl Sulfate on One-Year Adverse Events in Chronic Kidney Disease Patients with Heart Failure
Authors: Iwasaki Keiichiro , Miyoshi Toru , Urabe Chikara , Sakuragi Satoru , Kawai Yusuke , Fuke Soichiro , Doi Masayuki , Takaishi Atsushi , Oka Takefumi , Tokunaga Naoto , Ito Hiroshi
Published: July 2024

Background/Objectives: Indoxyl sulfate, a uremic toxin, is associated with mortality and cardiovascular events in patients with chronic kidney disease (CKD). This study aimed to evaluate the prognostic implications of serum indoxyl sulfate levels in patients with heart failure and CKD. Methods and Results: This was a prospective multicenter observational study. Overall, 300 patients with chronic heart failure with a previous history of hospitalization and an estimated glomerular filtration rate (eGFR) of 45 mL/min/1.73 m2 or less (CKD stage G3b to G5) without dialysis were analyzed. The primary outcome assessed in a time-to-event analysis from the measurement of indoxyl sulfate was a composite of all-cause death, hospitalization for heart failure, nonfatal myocardial infarction, and nonfatal stroke. Clinical events were followed-up to one year after indoxyl sulfate measurement. The median patient age was 75 years, and 57% of the patients were men. We divided the cohort into low and high indoxyl sulfate categories according to a median value of 9.63 mg/mL. The primary outcome occurred in 27 of 150 patients (18.0%) in the low indoxyl sulfate group and 27 of 150 patients (18.0%) in the high indoxyl sulfate group (hazard ratio, 1.00; 95% confidence interval, 0.58 to 1.70, p = 0.99). In the post hoc exploratory analyses, the results were consistent across age, sex, body mass index, left ventricular ejection fraction, eGFR, and N-terminal pro b-type natriuretic peptide. Conclusions: Among heart failure patients with CKD stages G3b to 5G, serum indoxyl sulfate concentrations were not significantly associated with the subsequent occurrence of cardiovascular events.