bluuebunny/crossref_metadata_2025 huggingface dataset:
Surveillance of Erythrovirus B19 (B19V) in patients with acute febrile illness suspected of arboviruses in Mato Grosso do Sul state, Brazil
Authors: Lichs Gislene Garcia C. , Fernandez Zoraida del Carmen , Nascimento Valdinete Alves do , Alcantara Daniel Maximo Corrêa , Lemos Everton Ferreira , Carvalho Cristiano M. Espínola , Demarchi Luiz Henrique Ferraz , Gonçalves Crhistinne Carvalho Maymone , Naveca Felipe Gomes , Favacho Alexsandra Rodrigues de Mendonça
Published: July 2024

Introduction

Human Erythrovirus (parvovirus) B19 infection can produce symptoms similar to those produced by Dengue, Chikungunya, and Zika viruses, making clinical diagnosis difficult. The importance of erythrovirus B19 in human pathology has been increased and reported in numerous studies published globally.

Methods

The B19V infection was investigated by real-time PCR in sera samples from patients with signs and symptoms related to classic arboviral symptoms. This study was conducted to provide information on the genetic diversity of Human Erythrovirus B19 (B19V) circulating in the state of Mato Grosso do Sul, Midwest region of Brazil, from 2017 to 2022. A total of 773 sera samples of patients with negative diagnostic results for Dengue, Chikungunya, and Zika, during the study period were analyzed.

Results

Erythrovirus DNA was found in 10.6% (82/773) of patients, among them 10 were pregnant women. Four samples were completely sequenced, and the other five partially, to genotype by phylogenetic reconstruction. All samples belong to worldwide dispersed genotype 1, subgenotype 1a.

Discussion

The findings of the study demonstrate the importance of including B19V in differential laboratory diagnosis for epidemiological purposes and appropriate patient management. The diagnosis for B19V should be performed, particularly among pregnant women, immunocompromised patients, and individuals with hemolytic diseases, given that the infection is more severe in these cases.
Bioenzymatic detoxification of mycotoxins
Authors: Liu Mengyu , Zhang Xue , Luan Haoni , Zhang Yue , Xu Wei , Feng Wei , Song Peng
Published: July 2024

Mycotoxins are secondary metabolites produced during the growth, storage, and transportation of crops contaminated by fungi and are physiologically toxic to humans and animals. Aflatoxin, zearalenone, deoxynivalenol, ochratoxin, patulin, and fumonisin are the most common mycotoxins and can cause liver and nervous system damage, immune system suppression, and produce carcinogenic effects in humans and animals that have consumed contaminated food. Physical, chemical, and biological methods are generally used to detoxify mycotoxins. Although physical methods, such as heat treatment, irradiation, and adsorption, are fast and simple, they have associated problems including incomplete detoxification, limited applicability, and cause changes in food characteristics (e.g., nutritive value, organoleptic properties, and palatability). Chemical detoxification methods, such as ammonification, ozonation, and peroxidation, pollute the environment and produce food safety risks. In contrast, bioenzymatic methods are advantageous as they achieve selective detoxification and are environmentally friendly and reusable; thus, these methods are the most promising options for the detoxification of mycotoxins. This paper reviews recent research progress on common mycotoxins and the enzymatic principles and mechanisms for their detoxification, analyzes the toxicity of the degradation products and describes the challenges faced by researchers in carrying out enzymatic detoxification. In addition, the application of enzymatic detoxification in food and feed is discussed and future directions for the development of enzymatic detoxification methods are proposed for future in-depth study of enzymatic detoxification methods.
Usefulness of cerebrospinal fluid presepsin (soluble CD14 subtype) as a new marker in the diagnosis of neurosurgical postoperative meningitis
Authors: Fuchinoue Yutaka , Kondo Kosuke , Sakaeyama Yuki , Nakada Chie , Terazono Sayaka , Kubota Syuhei , Mikai Masataka , Abe Mituyoshi , Ujiie Shinji , Morita Toshisuke , Sugo Nobuo
Published: July 2024

Objective

To determine the usefulness of cerebrospinal fluid (CSF) presepsin in the diagnosis of neurosurgical postoperative meningitis (POM).

Methods

The study included patients admitted to the Department of Neurosurgery, Toho University Medical Center Omori Hospital from May 1, 2020 to March 31, 2022 with suspected meningitis after neurosurgery who clinically required CSF sampling and patients who underwent CSF sampling for examination of idiopathic normal pressure hydrocephalus (iNPH). Participants were divided into a POM and a postoperative non meningitis (PONM) group based on the POM diagnostic criteria established for this study. The control group included patients from whom a CSF sample for iNPH was collected by tap test.

Results

A total of 238 CSF samples were collected from 90 patients. There were 39 samples in the POM, 180 samples in the PONM, and 19 samples in the control group. CSF presepsin levels in the POM were significantly higher than in the PONM group (1764.5 and 440.9 pg./mL, respectively; p < 0.0001). The control group had CSF presepsin levels of 95.5 pg./mL. A cutoff value of 669 pg./mL for CSF presepsin in POM and PONM groups had 76.9% sensitivity and 78.3% specificity for the diagnosis of POM. In analyzes including only subarachnoid hemorrhage (SAH) cases (123 samples), CSF presepsin (1251.2 pg./mL) in the POM was significantly higher than in the PONM subgroup (453.9 pg./mL; p < 0.0001). The cutoff value for presepsin in CSF among patients with SAH (669 pg./mL) had 87.5% sensitivity and 76.6% specificity, similar to that of all patients.

Conclusion

CSF presepsin is a useful marker in the diagnosis of neurosurgical POM even in patients with blood components, such as SAH. When POM is suspected, measurement of CSF presepsin may be recommended in addition to a general CSF examination.
Drosophila larval motor patterning relies on regulated alternative splicing of Dscam2
Authors: Odierna G. Lorenzo , Kerwin Sarah K. , Shin Grace Ji-eun , Millard S. Sean
Published: July 2024

Recent studies capitalizing on the newly complete nanometer-resolution Drosophila larval connectome have made significant advances in identifying the structural basis of motor patterning. However, the molecular mechanisms utilized by neurons to wire these circuits remain poorly understood. In this study we explore how cell-specific expression of two Dscam2 isoforms, which mediate isoform-specific homophilic binding, contributes to motor patterning and output of Drosophila larvae. Ablating Dscam2 isoform diversity resulted in impaired locomotion. Electrophysiological assessment at the neuromuscular junction during fictive locomotion indicated that this behavioral defect was largely caused by weaker bouts of motor neuron activity. Morphological analyses of single motor neurons using MultiColour FlpOut revealed severe errors in dendrite arborization and assessment of cholinergic and GABAergic projections to the motor domain revealed altered morphology of interneuron processes. Loss of Dscam2 did not affect locomotor output, motor neuron activation or dendrite targeting. Our findings thus suggest that locomotor circuit phenotypes arise specifically from inappropriate Dscam2 interactions between premotor interneurons and motor neurons when they express the same isoform. Indeed, we report here that first-order premotor interneurons express Dscam2A. Since motor neurons express Dscam2B, our results provide evidence that Dscam2 isoform expression alternates between synaptic partners in the nerve cord. Our study demonstrates the importance of cell-specific alternative splicing in establishing the circuitry that underlies neuromotor patterning without inducing unwanted intercellular interactions.
Association between dietary niacin intake and risk of Parkinson’s disease in US adults: cross-sectional analysis of survey data from NHANES 2005–2018
Authors: Zhang Ling , Yang Shaojie , Liu Xiaoyan , Wang Chunxia , Tan Ge , Wang Xueping , Liu Ling
Published: July 2024

Parkinson’s disease (PD) is one of the most common neurodegenerative diseases and involves various pathogenic mechanisms, including oxidative stress and neuroinflammation. Niacin, an important cofactor in mitochondrial energy metabolism, may play a key role in the pathogenesis of PD. An in-depth exploration of the relationship between niacin and mitochondrial energy metabolism may provide new targets for the treatment of PD. The present study was designed to examine the association between dietary niacin intake and the risk of PD in US adults. Data from adults aged 40 years and older collected during cycles of the United States (US) National Health and Nutrition Examination Survey (NHANES) from 2005 to 2018 were used. A multiple logistic regression model was used to analyze the relationship between dietary niacin intake and the risk of PD. Further linear tests using restricted cubic splines (RCS) were performed to explore the shape of the dose–response relationship. Subgroup stratification and interaction analyses were conducted according to years of education, marital status, smoking, and hypertension to evaluate the stability of the association between different subgroups. A total of 20,211 participants were included in this study, of which 192 were diagnosed with PD. In the fully adjusted multiple logistic regression model, dietary niacin intake was negatively associated with the risk of PD (OR: 0.77, 95%CI: 0.6–0.99; p = 0.042). In the RCS linear test, the occurrence of PD was negatively correlated with dietary niacin intake (nonlinearity: p = 0.232). In stratified analyses, dietary niacin intake was more strongly associated with PD and acted as an important protective factor in patients with fewer years of education (OR: 0.35, 95%CI: 0.13–0.93), married or cohabitating (OR: 0.71, 95%CI: 0.5–0.99), taking dietary supplements (OR: 0.6, 95%CI: 0.37 0.97), non-smokers (OR: 0.57, 95%CI: 0.39–0.85), those with hypertension (OR: 0.63, 95%CI: 0.63–0.95), coronary artery disease (OR: 0.77, 95%CI: 0.6–1), and stroke (OR: 0.75, 95%CI: 0.88–0.98), but the interaction was not statistically significant in all subgroups. Dietary niacin intake was inversely associated with PD risk in US adults, with a 23% reduction in risk for each 10 mg increase in niacin intake.
Determining amino acid requirements in humans
Authors: Paoletti Alyssa , Courtney-Martin Glenda , Elango Rajavel
Published: July 2024

Amino acids form the building blocks of body protein. Dietary protein sources provide the amino acids needed, but protein sources vary widely in amio acid composition. To ensure humans can meet body demands for amino acids, amino acid intake recommendations are provided by the Dietary Reference Intakes (DRI) and by Food and Agriculture Organization/World Health Organization/United Nations University (FAO/WHO/UNU). Current amino acid intake recommendations, however, are based on data collected predominantly from young adult males. The development of the minimally invasive indicator amino acid oxidation (IAAO) method has permitted the evaluation of amino acid requirements in various vulnerable populations. The purpose of this review is to discuss recent amino acid requirement studies in school-age children, pregnant females and the elderly determined using the IAAO technique. These requirements will help to inform evidence-based recommendations that will help to guide dietary guidelines.
Prediction of overall survival in patients with locally advanced pancreatic cancer using longitudinal diffusion-weighted MRI
Authors: Bisgaard Anne L. H. , Brink Carsten , Schytte Tine , Bahij Rana , Weisz Ejlsmark Mathilde , Bernchou Uffe , Bertelsen Anders S. , Pfeiffer Per , Mahmood Faisal
Published: July 2024

Background and purpose

Biomarkers for prediction of outcome in patients with pancreatic cancer are wanted in order to personalize the treatment. This study investigated the value of longitudinal diffusion-weighted magnetic resonance imaging (DWI) for prediction of overall survival (OS) in patients with locally advanced pancreatic cancer (LAPC) treated with stereotactic body radiotherapy (SBRT).

Materials and methods

The study included 45 patients with LAPC who received 5 fractions of 10 Gy on a 1.5T MRI-Linac. DWI was acquired prior to irradiation at each fraction. The analysis included baseline values and time-trends of the apparent diffusion coefficient (ADC) and DWI parameters obtained using a decomposition method. A multivariable Cox proportional hazards model for OS was made using best-subset selection, using cross-validation based on Bootstrap.

Results

The median OS from the first day of SBRT was 15.5 months (95% CI: 13.2-20.6), and the median potential follow-up time was 19.8 months. The best-performing multivariable model for OS included two decomposition-based DWI parameters: one baseline and one time-trend parameter. The C-Harrell index describing the model’s discriminating power was 0.754. High baseline ADC values were associated with reduced OS, whereas no association between the ADC time-trend and OS was observed.

Conclusion

Decomposition-based DWI parameters indicated value in the prediction of OS in LAPC. A DWI time-trend parameter was included in the best-performing model, indicating a potential benefit of acquiring longitudinal DWI during the SBRT course. These findings support both baseline and longitudinal DWI as candidate prognostic biomarkers, which may become tools for personalization of the treatment of patients with LAPC.
Major response of a peritoneal mesothelioma to nivolumab and ipilimumab: a case report, molecular analysis and review of literature
Authors: Reveneau Marie-Florence , Masliah-Planchon Julien , Fernandez Manuel , Ouikene Abdenour , Dron Bernard , Dadamessi Innocenti , Dayen Charles , Golmard Lisa , Chauffert Bruno
Published: July 2024

Malignant peritoneal mesothelioma (MPM) is a rare tumor associated with a poor prognosis and a lack of consensus regarding treatment strategies. While the Checkmate 743 trial demonstrated the superiority of first-line nivolumab and ipilimumab over chemotherapy in malignant pleural mesothelioma (MPlM), few studies have assessed the effectiveness of immunotherapy against MPM, due to its rarity. Here, we report a major and sustained 12-month response in a 74-year-old female patient who received the anti-PD-1 nivolumab and the anti-CTLA4 ipilimumab as first-line therapy for diffuse MPM. PD-L1 was expressed and BAP1 expression was lost, as shown by immunohistochemistry, however the BAP1 gene was not mutated. Our findings suggest a role for ICI in non-resectable diffuse MPM exhibiting PD-L1 overexpression and loss of BAP1 expression, and instill new hope in their treatment. To our knowledge, this is the second reported case of dual immunotherapy used as first-line in MPM with a major clinical response. To investigate the clinical outcome, we conducted additional molecular analyses of the MPM tumor and we reviewed the literature on immunotherapy in MPM to discuss the role of PD-L1 and BAP1.
Type II mode of JAK2 inhibition and destabilization are potential therapeutic approaches against the ruxolitinib resistance driven myeloproliferative neoplasms
Authors: Gorantla Sivahari P. , Prince Gerin , Osius Jasmin , Dinesh Dhurvas Chandrasekaran , Boddu Vijay , Duyster Justus , von Bubnoff Nikolas
Published: July 2024

Background

Ruxolitinib has been approved by the US FDA for the treatment of myeloproliferative neoplasms such as polycythemia vera and primary myelofibrosis. Ruxolitinib will remain a main stay in the treatment of MPN patients due to its effective therapeutic benefits. However, there have been instances of ruxolitinib resistance in MPN patients. As JAK2 is a direct target of ruxolitinib, we generated ruxolitinib-resistant clones to find out the mechanism of resistance.

Methods

Cell-based screening strategy was used to detect the ruxolitinib-resistant mutations in JAK2. The Sanger sequencing method was used to detect the point mutations in JAK2. Mutations were re-introduced using the site-directed mutagenesis method and stably expressed in Ba/F3 cells. Drug sensitivities against the JAK2 inhibitors were measured using an MTS-based assay. JAK2 and STAT5 activation levels and total proteins were measured using immunoblotting. Computational docking studies were performed using the Glide module of Schrodinger Maestro software.

Results

In this study, we have recovered seven residues in the kinase domain of JAK2 that affect ruxolitinib sensitivity. All these mutations confer cross-resistance across the panel of JAK2 kinase inhibitors except JAK2-L983F. JAK2-L983F reduces the sensitivity towards ruxolitinib. However, it is sensitive towards fedratinib indicating that our screen identifies the drug-specific resistance profiles. All the ruxolitinib-resistant JAK2 variants displayed sensitivity towards type II JAK2 inhibitor CHZ-868. In this study, we also found that JAK1-L1010F (homologous JAK2-L983F) is highly resistant towards ruxolitinib suggesting the possibility of JAK1 escape mutations in JAK2-driven MPNs and JAK1 mutated ALL. Finally, our study also shows that HSP90 inhibitors are potent against ruxolitinib-resistant variants through the JAK2 degradation and provides the rationale for clinical evaluation of potent HSP90 inhibitors in genetic resistance driven by JAK2 inhibitors.

Conclusion

Our study identifies JAK1 and JAK2 resistance variants against the type I JAK2 inhibitors ruxolitinib, fedratinib, and lestaurtinib. The sensitivity of these resistant variants towards the type II JAK2 inhibitor CHZ-868 indicates that this mode of type II JAK2 inhibition is a potential therapeutic approach against ruxolitinib refractory leukemia. This also proposes the development of potent and specific type II JAK2 inhibitors using ruxolitinib-resistance variants as a prototype.
Effect of different sedatives on the prognosis of patients with mechanical ventilation: a retrospective cohort study based on MIMIC-IV database
Authors: Shi Xiaoding , Zhang Jiaxing , Sun Yufei , Chen Meijun , Han Fei
Published: July 2024

Aim

To compare the effects of midazolam, propofol, and dexmedetomidine monotherapy and combination therapy on the prognosis of intensive care unit (ICU) patients receiving continuous mechanical ventilation (MV).

Methods

11,491 participants from the Medical Information Mart for Intensive Care (MIMIC)-IV database 2008–2019 was included in this retrospective cohort study. The primary outcome was defined as incidence of ventilator-associated pneumonia (VAP), in-hospital mortality, and duration of MV. Univariate and multivariate logistic regression analyses were utilized to evaluate the association between sedation and the incidence of VAP. Univariate and multivariate Cox analyses were performed to investigate the correlation between sedative therapy and in-hospital mortality. Additionally, univariate and multivariate linear analyses were conducted to explore the relationship between sedation and duration of MV.

Results

Compared to patients not receiving these medications, propofol alone, dexmedetomidine alone, combination of midazolam and dexmedetomidine, combination of propofol and dexmedetomidine, combination of midazolam, propofol and dexmedetomidine were all association with an increased risk of VAP; dexmedetomidine alone, combination of midazolam and dexmedetomidine, combination of propofol and dexmedetomidine, combination of midazolam, propofol and dexmedetomidine may be protective factor for in-hospital mortality, while propofol alone was risk factor. There was a positive correlation between all types of tranquilizers and the duration of MV. Taking dexmedetomidine alone as the reference, all other drug groups were found to be associated with an increased risk of in-hospital mortality. The administration of propofol alone, in combination with midazolam and dexmedetomidine, in combination with propofol and dexmedetomidine, in combination with midazolam, propofol and dexmedetomidine were associated with an increased risk of VAP compared to the use of dexmedetomidine alone.

Conclusion

Dexmedetomidine alone may present as a favorable prognostic option for ICU patients with mechanical ventilation MV.
PD-1 inhibitors in advanced esophageal squamous cell carcinoma: a survival analysis of reconstructed patient-level data
Authors: Yan Chunyan , Cao Wenxiu , Li Jianghua , Zhang Lei , Diao Ruigang
Published: July 2024

Background

Recently, a sum of trials of programmed cell death-1 (PD-1) inhibitors combined with chemotherapy have shown excellent efficacy compared to chemotherapy alone in patients with previously untreated, advanced esophageal squamous cell carcinoma (ESCC). However, there is no head-to-head comparison and consensus on which immunotherapy regimen results in better survival outcomes. This study aimed to evaluate the survival efficacy of various PD-1 inhibitor–based therapies in the first-line treatments for patients with advanced ESCC.

Methods

Data collected prior to 31 July 2023 were searched in the PubMed, Cochrane Library, Embase, Medline, and Web of Science databases. Overall survival (OS) and progression-free survival curves were pooled using the MetaSurv package. Survival data were compared by reconstructed individual patient data.

Results

A total of 4,162 patients and seven randomized controlled trials were included. After synthesizing, PD-1 inhibitors prolonged median OS from 11.3 months (95% CI (confidence interval) 10.7–11.7) to 15.6 months (95% CI 14.7–16.3). Based on reconstructed patient-level data, the toripalimab, tislelizumab, and sintilimab group achieved the longest OS, whereas the sintilimab and tislelizumab group had the lowest risk of recurrence than other treatments. In patients with a combined positive score of ≥10, sintilimab had better OS efficacy than pembrolizumab (HR: 0.71, 95% CI: 0.52–0.96). In terms of tumor proportion score of ≥1%, camrelizumab, nivolumab, and toripalimab showed proximate survival benefits in both OS and progression-free survival.

Conclusion

PD-1 inhibitor combined with chemotherapy significantly improved the survival time of patients with advanced ESCC. Toripalimab, tislelizumab, and sintilimab plus chemotherapy showed the best OS benefit. Longer progression-free benefits might be generated from adding tislelizumab and sintilimab to chemotherapy. Sintilimab was strongly recommended for patients with high programmed cell death–ligand 1 abundance.

Systematic Review Registration:

[https://www.crd.york.ac.uk/PROSPERO/], identifier [CRD42024501086].
Phosphodiesterase 7 inhibitor reduces stress-induced behavioral and cytoarchitectural changes in C57BL/6J mice by activating the BDNF/TrkB pathway
Authors: Dong Jiahao , Wei Ran , Zong Fangjiao , Wang Zhe , Ma Shengyao , Zhao Wei , Lin Yuanyuan , Zhang Aixin , Lan Ge , Zhang Fang , Zhang Han-Ting
Published: July 2024

Background

Phosphodiesterase 7 (PDE7) plays a role in neurological function. Increased expression and activity of PDE7 has been detected in several central nervous system diseases. However, the role of PDE7 in regulating stress levels remains unclear. Thus, this study aimed to determine whether and how PDE7 involved in the stress-induced behavioral and neuron morphological changes.

Methods

The single prolonged stress (SPS) was used to build a stress exposure model in C57BL/6 J mice and detected PDE7 activity in hippocampus, amygdala, prefrontal cortex and striatum. Next, three doses (0.2, 1, and 5 mg/kg) of the PDE7 inhibitor BRL-50481 were intraperitoneally administered for 10 days, then behavioral, biochemical, and morphological tests were conducted.

Results

PDE7 activity in hippocampus of mice significantly increased at all times after SPS. BRL-50481 significantly attenuated SPS induced anxiety-like behavior and fear response in both context and cue. In addition, BRL-50481 increased the levels of key molecules in the cAMP signaling pathway which were impaired by SPS. Immunofluorescent staining and Sholl analysis demonstrated that BRL-50481 also restored the nucleus/cytoplasm ratio of hippocampal neurons and improved neuronal plasticity. These effects of BRL-50481 were partially blocked by the TrkB inhibitor ANA-12.

Conclusion

PDE7 inhibitors attenuate stress-induced behavioral changes by protecting the neuron cytoarchitecture and the neuronal plasticity in hippocampus, which is mediated at least partly through the activation of BDNF/TrkB signaling pathway. These results proved that PDE7 is a potential target for treating stress-induced behavioral and physiological abnormalities.
Role of macrophage polarization in heart failure and traditional Chinese medicine treatment
Authors: Zhu Zheqin , Wang Min , Lu Shenghua , Dai Sisi , Liu Jianhe
Published: July 2024

Heart failure (HF) has a severe impact on public health development due to high morbidity and mortality and is associated with imbalances in cardiac immunoregulation. Macrophages, a major cell population involved in cardiac immune response and inflammation, are highly heterogeneous and polarized into M1 and M2 types depending on the microenvironment. M1 macrophage releases inflammatory factors and chemokines to activate the immune response and remove harmful substances, while M2 macrophage releases anti-inflammatory factors to inhibit the overactive immune response and promote tissue repair. M1 and M2 restrict each other to maintain cardiac homeostasis. The dynamic balance of M1 and M2 is closely related to the Traditional Chinese Medicine (TCM) yin-yang theory, and the imbalance of yin and yang will result in a pathological state of the organism. Studies have confirmed that TCM produces positive effects on HF by regulating macrophage polarization. This review describes the critical role of macrophage polarization in inflammation, fibrosis, angiogenesis and electrophysiology in the course of HF, as well as the potential mechanism of TCM regulation of macrophage polarization in preventing and treating HF, thereby providing new ideas for clinical treatment and scientific research design of HF.
Growth and non-structural carbohydrates response patterns of Eucommia ulmoides under salt and drought stress
Authors: Zhang Xuejie , Qin Hao , Kan Zhenchao , Liu Dan , Wang Bingxin , Fan Shoujin , Jiang Peipei
Published: July 2024

Introduction

Salinity and droughts are severe abiotic stress factors that limit plant growth and development. However, the differences and similarities of non-structural carbohydrates (NSCs) responses patterns of trees under the two stress conditions remain unclear.

Methods

We determined and compared the growth, physiology, and NSCs response patterns and tested the relationships between growth and NSCs concentrations (or pool size) of Eucommia ulmoides seedlings planted in field under drought and salt stress with different intensities and durations.

Results and discussion

We found that drought and salt stress can inhibit the growth of E. ulmoides, and E. ulmoides tended to enhance its stress resistance by increasing proline concentration and leaf thickness or density but decreasing investment in belowground biomass in short-term stress. During short-term drought and salt stress, the aboveground organs showed different NSCs response characteristics, while belowground organs showed similar change characteristics: the starch (ST) and NSCs concentrations in the coarse roots decreased, while the ST and soluble sugar (SS) concentrations in the fine roots increased to enhance stress resistance and maintain water absorption function. As salt and drought stress prolonged, the belowground organs represented different NSCs response patterns: the concentrations of ST and SS in fine roots decreased as salt stress prolonged; while ST in fine roots could still be converted into SS to maintain water absorption as drought prolonged, resulting in an increase of SS and a decrease of ST. Significant positive relationships were found between growth and the SS and total NSCs concentrations in leaves and branches, however, no significant correlations were found between growth and below-ground organs. Moreover, relationships between growth and NSCs pool size across organs could be contrast.

Conclusion

Our results provide important insights into the mechanisms of carbon balance and carbon starvation and the relationship between tree growth and carbon storage under stress, which were of great significance in guiding for the management of artificial forest ecosystem under the context of global change.
Parenting style and the non-cognitive development of high school student: evidence from rural China
Authors: Wang Sangui , Zheng Lijuan
Published: July 2024

Introduction

Understanding the relationship between parenting style and the non-cognitive development of high school students is crucial, particularly in rural China. Non-cognitive abilities, including traits such as emotional regulation, resilience, and interpersonal skills, play a significant role in students’ overall development and future success. This study aims to investigate how different parenting styles impact non-cognitive abilities among high school students in rural China.

Methods

This study surveyed 6,549 high school students and their primary caregivers in rural China. The students had an average age of 17.61 years, with 48% being male, and 62% of Han ethnicity. Primary caregivers self-reported their parenting styles, while the students’ non-cognitive abilities were assessed using the Big Five Inventory-Short (BFI-S). The relationship between parenting style and non-cognitive development was analyzed using two distinct methods: two dimensions (authoritative and authoritarian) and four categories of parenting styles.

Results

The study revealed that an authoritative parenting style had a positive impact on the non-cognitive abilities of students. Conversely, a negative association was observed between the authoritarian parenting style and the students’ non-cognitive development. This association was more pronounced in the non-cognitive developmental scores of girls compared to boys. Additionally, parents from wealthier families or those with higher levels of education were more likely to adopt an authoritative parenting style rather than an authoritarian one.

Discussion

The results of this study highlight the significant influence of parenting styles on the non-cognitive development of high school students in rural China. Authoritative parenting, characterized by warmth and structure, appears to foster better non-cognitive outcomes, while authoritarian parenting, marked by strictness and less warmth, is associated with poorer non-cognitive development. The gender differences observed suggest that girls may be more sensitive to variations in parenting style. Furthermore, the socioeconomic and educational background of parents plays a crucial role in determining the parenting style adopted. These findings underscore the importance of developing and implementing parenting training interventions in rural China, aimed at promoting authoritative parenting practices to enhance the non-cognitive development of students.
Epigenetic associations of GPNMB rs199347 variant with alcohol consumption in Parkinson’s disease
Authors: Chen Yen-Chung , Liaw Yi-Chia , Nfor Oswald Ndi , Hsiao Chih-Hsuan , Zhong Ji-Han , Wu Shey-Lin , Liaw Yung-Po
Published: July 2024

Introduction

Alcohol consumption can induce a neuroinflammatory response and contribute to the progression of neurodegeneration. However, its association with Parkinson’s disease (PD), the second most common neurodegenerative disorder, remains undetermined. Recent studies suggest that the glycoprotein non-metastatic melanoma protein B (GPNMB) is a potential biomarker for PD. We evaluated the association of rs199347, a variant of the GPNMB gene, with alcohol consumption and methylation upstream of GPNMB.

Methods

We retrieved genetic and DNA methylation data obtained from participants enrolled in the Taiwan Biobank (TWB) between 2008 and 2016. After excluding individuals with incomplete or missing information about potential PD risk factors, we included 1,357 participants in our final analyses. We used multiple linear regression to assess the association of GPNMB rs199347 and chronic alcohol consumption (and other potential risk factors) with GPNMB cg17274742 methylation.

Results

There was no difference between the distribution of GPNMB rs199347 genotypes between chronic alcohol consumers and the other study participants. A significant interaction was observed between the GPNMB rs199347 variant and alcohol consumption (p = 0.0102) concerning cg17274742 methylation. Compared to non-chronic alcohol consumers with the AA genotype, alcohol drinkers with the rs199347 GG genotype had significantly lower levels (hypomethylation) of cg17274742 (p = 0.0187).

Conclusion

Alcohol consumption among individuals with the rs199347 GG genotype was associated with lower levels of cg17274742 methylation, which could increase expression of the GPNMB gene, an important neuroinflammatory-related risk gene for PD.
Knowledge, attitude and practice regarding constipation in pregnancy among pregnant women in Shanghai: a cross-sectional study
Authors: Lin Lin , Yu Yi , Gu Weirong , Hu Rong , Zhu Hao
Published: July 2024

Objective

This study aims to investigate the Knowledge, Attitude, and Practice (KAP) pertaining to constipation during pregnancy among pregnant women in Shanghai.

Methods

Demographic data and KAP scores were collected using a questionnaire. Differences across groups were analyzed using either Wilcoxon-Mann–Whitney tests or Kruskal-Wallis analysis of variance. Spearman’s correlation analysis was utilized to evaluate the relationships between KAP scores. Multivariable logistic regression analyses were conducted to identify factors that influence KAP scores.

Results

Encompassing 241 individuals (46.6%) aged between 30 and 34 years, with 349 participants (67.5%) being nulliparous. The median scores for knowledge (possible range: 0–26), attitude (possible range: 7–35), and practice (possible range: 14–70) were 22 (18, 24), 26 (23, 29), and 51 (46, 56), respectively. Multivariate analysis indicated that being a medical professional (OR = 2.222, p = 0.043) and receiving education on constipation during pregnancy (OR = 0.432, p < 0.001) were significantly associated with higher knowledge scores. Factors significantly associated with practice included being aged 30–34 years (OR = 2.745, p < 0.001), aged 35 years and above (OR = 2.514, p < 0.001), working in education (OR = 2.310, p = 0.012), and not experiencing constipation before pregnancy (OR = 1.894, p = 0.001).

Conclusion

Pregnant women demonstrated satisfactory knowledge, positive attitudes, and proactive practices concerning constipation during pregnancy. To further augment clinical practice, healthcare providers should tailor educational interventions and guidance specifically for pregnant women who are not medical professionals and those who have not received education and guidance related to constipation during pregnancy.
The relationship between neighborhood economic deprivation and community-acquired pneumonia related admissions in Maryland
Authors: Akinyemi Oluwasegun , Fasokun Mojisola , Odusanya Eunice , Weldeslase Terhas , Omokhodion Ofure , Michael Miriam , Hughes Kakra
Published: July 2024

Introduction

Community-acquired pneumonia (CAP) is a major health concern in the United States (US), with its incidence, severity, and outcomes influenced by social determinants of health, including socioeconomic status. The impact of neighborhood socioeconomic status, as measured by the Distressed Communities Index (DCI), on CAP-related admissions remains understudied in the literature.

Objective

To determine the independent association between DCI and CAP-related admissions in Maryland.

Methods

We conducted a retrospective study using the Maryland State Inpatient Database (SID) to collate data on CAP-related admissions from January 2018 to December 2020. The study included adults aged 18–85 years. We explored the independent association between community-level economic deprivation based on DCI quintiles and CAP-related admissions, adjusting for significant covariates.

Results

In the study period, 61,467 cases of CAP-related admissions were identified. The patients were predominantly White (49.7%) and female (52.4%), with 48.6% being over 65 years old. A substantive association was found between the DCI and CAP-related admissions. Compared to prosperous neighborhoods, patients living in economically deprived communities had 43% increased odds of CAP-related admissions.

Conclusion

Residents of the poorest neighborhoods in Maryland have the highest risk of CAP-related admissions, emphasizing the need to develop effective public health strategies beneficial to the at-risk patient population.
Drivers and collaborative governance of public health emergency response in the context of digital city
Authors: Chen Yang , Yu Yu
Published: July 2024

Introduction

With the frequent occurrence of public health events, the government inevitably makes many mistakes in emergency management. In modern emergency management, it is particularly important to promote the diversification of emergency management subjects and improve the government’s emergency management ability.

Methods

In order to make up for the deficiency of government’s participation in public health emergency management, this paper analyzes the driving factors and driving effects of enterprises’ participation in public health emergency response under the background of digital city. A fully explained structural model is used to analyze the relationship between the different drivers. In addition, the spatial and temporal distribution characteristics of public health events were analyzed through spatial auto-correlation. On this basis, the government cooperative governance strategy is discussed.

Results and discussion

The results show that in the context of digital cities, there are 14 driving factors for enterprises to participate in public health emergency response. The most important factors are the company’s own development needs, relative technical advantages and so on. The driving efficiency is mainly concentrated in three aspects: psychology, resources and structure. Public health events have periodicity in time distribution and regional differences in spatial distribution. The significance of this study is to help the government improve the emergency management ability from different angles.
Profiles of physical frailty, social frailty, and cognitive impairment among older adults in rural areas of China: a latent profile analysis
Authors: Dong Qian , Bu Xiaolong , Wang Ting , Liu Man , Zhong Feng , Liu Cuiping
Published: July 2024

Background

As China rapidly ages, it has now become a deeply aging society with the largest number of older individuals in the world. The issue is particularly severe in rural areas. With the aging population growing and the older population expanding, health problems are becoming more prevalent among older individuals, particularly frailty and cognitive impairments. This study aimed to identify the profiles of physical frailty, social frailty, and cognitive impairment among older adults and explore the influencing factors.

Methods

In this cross-sectional study, participants were recruited from six villages in four cities in Shandong Province, China from July to October 2023 through cluster random sampling. Latent profile analysis was used to determine the profiles of physical frailty, social frailty, and cognitive impairment. Chi-square tests and Mann–Whitney U tests were used for univariate analysis, while binary logistic regression was used to analyze the related factors.

Results

Seven hundred and sixty-nine older adult care in rural areas showed two profiles: the “high cognitive function and low frailty” group (73.7%, n = 567) and the “low cognitive function and high frailty” group (26.3%, n = 202). A binary logistic regression found that older people were more likely to be aged 80 or older (OR = 2.253, p = 0.029), have a low income level (OR = 1.051, p = 0.007), have one or two (OR = 2.287, p = 0.004), or more than three chronic diseases (OR = 3.092, p = 0.002), and report moderate (OR = 3.406, p = 0.024) or poor health status (OR = 9.085, p < 0.001) in the “low cognitive function and high frailty” group. Meanwhile, older adults who have completed high school (OR = 0.428, p = 0.005) or junior college and above (OR = 0.208, p = 0.009), and engage in adequate physical activity (OR = 0.319, p < 0.001) were more likely to be in the “high cognitive function and low frailty” group.

Conclusion

In the future, medical professors should increasingly prioritize promptly identifying and intervening in cognitive decline and frailty status in older individuals without delay.
From waste to wealth: a study of concrete recycling in Jordan
Authors: Al-Sharif Mutasem , Geldermans Bob , Rinke Mario
Published: July 2024

The construction industry in Jordan plays a pivotal role in the national economy but also generates a significant amount of concrete waste, leading to environmental and health challenges. Current waste management practices involve landfill use and unregulated disposal, demonstrating the country's infrastructural shortcomings in concrete waste handling. Despite the recognized importance of the construction sector and the challenges of concrete waste management, more comprehensive studies need to focus on the practicality and benefits of recycling concrete within a circular economy framework in Jordan. This gap hinders the development of effective strategies that could mitigate environmental impacts, promote sustainability, and leverage economic and social benefits in the construction industry. Given the accelerating urbanization, increasing environmental awareness, and the pressing need for sustainable development goals, the timing for this study is critical. It aims to catalyze a timely shift toward more sustainable waste management practices that align with global sustainability trends and national priorities. This study aims to fill the knowledge gap regarding the practicality of recycling concrete within a circular economy context in Jordan and explore the hypothesis that adopting circular economy principles can enhance resource efficiency, reduce waste, lower greenhouse gas emissions, foster economic benefits, and create job opportunities within the sector. Methodologically, this research comprises an analysis based on literature, international comparisons, and technical, economic, and ecological explorations of concrete recycling in Jordan. The literature study establishes a foundational understanding of the current waste management landscape, the anticipated benefits of concrete recycling, and the challenges to its implementation. Furthermore, a technical analysis evaluates the quality and applicability of recycled concrete. The in-depth examination of Jordan's concrete waste problem highlights the urbanization challenges, regulatory gaps, and environmental, social, and economic impacts. Barriers, solutions, and recommendations are discussed through an interdisciplinary lens. This integrated approach supports the transition toward a sustainable, environmentally responsible, and economically viable construction sector. The findings could contribute to policy-making and the advancement of industry practices, ultimately promoting sustainable development in Jordan.
The arena or E-games triathlon as a unique real world and virtual mixed-model endurance sports event
Authors: Stapley Paul J. , Lepers Romuald , Heming Tim , Gremeaux Vincent
Published: July 2024

The sport of triathlon has evolved to become a discipline comprising races of different lengths and formats. It has also undergone significant growth in popularity and exposure with several variations in format from the classic swim – bike – run combination attracting significant television and media coverage. Since 2021 an original and unique format called the Arena Games Triathlon (or in 2024 the E-Games) has evolved that involves athletes competing against each other in swimming, cycling and running combining both the real and virtual worlds in one race. This model of endurance sport is currently unique, provides instantaneous data on performance and has the potential to be a tool for performance prediction, talent selection and sport development. The goal of this perspective paper is to provide context around the emergence of the Arena Games Triathlon series, describe the format of this type of racing, outline how it has the potential to drive training and evaluation of triathletes and discuss the attractiveness of its future inclusion as an Olympic discipline.
Do homegardens act as agent of agrobiodiversity conservation: a case study of homegardens of diverse socio-ecological zones in the Brahmaputra Valley, Assam
Authors: Sharma Rashmita , Mina Usha , Devi Ashalata , Kumar B. Mohan
Published: July 2024

Introduction

Homegardens are one of the oldest agroforestry systems reported around the world. These agroforestry systems are often reported as harbingers of plant biodiversity conservation. However, a comprehensive understanding of these systems from the perspective of species level agrobiodiversity conservation is often missing.

Methodology

This study first visualizes the comprehensive role of homegardens in species level agrobiodiversity conservation and then assesses any variation in agrobiodiversity along diverse Socio-ecological Zones (SEZs) in the study site. The prominent SEZs identified in the study site were Protected Area (PA), Riverine (RI), Rural Market (RM), and Tea Estate (TE). Eight ethnic/linguistic groups were also identified at the study site. Agrobiodiversity inventorying of 192 homegardens from 16 villages was done.

Results

The results of the study highlight that homegardens in the study site have high species level agrobiodiversity concentration (101 total tree species reported, 39.58% of homegardens (HGs) had more than 10 varieties of vegetables, 68% had atleast one variety of bamboo, 76% had atleast one banana variety, 20.83% had pond). A total of 64% of HGs had livestock and around 85% had poultry. Moreover, this agrobiodiversity distribution also varied along different SEZs. The livestock diversity indices ranged from 0.49 (TE) to 1.04 (PA). The average plant diversity among homegarden was found to be in the range of 1.09 (PA) to 1.48 (TE) for Shannon, 0.45 (PA) to 0.66 (TE) for Simpson, 0.31 (PA) to 0.71 (TE) for Pileou evenness and 2.39 (PA) to 2.76 (RM) for Margalef. The plant composition reflected the dominance of the food species i.e. an average of 37% in each SEZ. Sorenson similarity index among different SEZs for plant and livestock was found to be highest between the HGs of the PA and RM (0.82). Among the ethnic/linguistic groups, the highest mean number of plant species (51) was found among the Mishing tribe. Also, high similarity index (0.78) was found in plant and livestock composition among the Mishing and the Bodo tribes.

Discussion

The findings imply that HGs exemplify diversified and integrated systems, showcasing their potential to play a crucial role in the development of sustainable food systems.