bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of HDAC inhibitors on neuroblastoma SH-SY5Y cell differentiation into mature neurons via the Wnt signaling pathway
Authors: Choi Jiyun , Hwang Jinsu , Mahesh Ramalingam , Jeong Han-Seong , Jang Sujeong
Published: December 2022

Histone deacetylase (HDAC) inhibitors affect cell homeostasis, gene expression, and cell cycle progression and promote cell terminal differentiation or apoptosis. However, the effect of HDAC inhibition on SH-SY5Y cells, neuroblastoma cells capable of differentiating into neurons under specific conditions, such as in presence of retinoic acid (RA), is unknown. In this study, we hypothesized that HDAC inhibitors induced the neuronal differentiation of SH-SY5Y cells. To test this hypothesis, we used phase contrast microscopy, immunocytochemistry (ICC), reverse transcriptase PCR (RT-PCR), and western blot analysis. MS-275 and valproic acid (VPA), two HDAC inhibitors, were selected to evaluate neuronal differentiation. It was confirmed that cells treated with MS-275 or VPA differentiated into mature neurons, distinguished by bipolar or multipolar morphologies with elongated branches. In addition, the mRNA expression of neuronal markers (MAP2 and NEFH), the astrocytic marker (GFAP), and the oligodendrocyte marker (CNP) was significantly increased with MS-275 or VPA treatment compared to RA treatment. Interestingly, the mRNA expression of NEFM, another neuronal marker, was only increased in VPA treatment compared to RA treatment. In addition, the protein expression of Tuj1 and NeuN, other neuronal markers, were highly expressed with HDAC inhibitors compared to RA treatment. Furthermore, we confirmed that non-canonical Wnt signaling was upregulated with HDAC inhibitors via MAPK signaling and the Wnt/JNK pathway. Therefore, both MS-275 and VPA promoted the differentiation of SH-SY5Y cells into mature neurons via the Wnt signaling pathway.
Structure elucidation and anti-Klebsiella activity with in silico ADME prediction and molecular docking of the bioactive metabolite from Aspergillus sp. 3MAG
Authors: awady Mohamed E. El , Abdulrazik Ghada , Hamed Ahmed A. , Hassan Mervat G. , Abdel-Monem Mohamed O.
Published: December 2022

Marine environments are considered an interesting source of new active ingredients. The discovery and subsequent clinical introduction of antibiotics is one of the most important game-changers in the history of medicine. So, A pure compound of Dibutyl phthalate ester was extracted from a marine-derived fungus of Aspergillussp. 3MAG isolated from soft coral collected from the Red Sea. The suppression of Klebsiella pneumoniae strain 3MAG by Dibutyl phthalate ester was an intriguing finding in our investigation measured by minimum inhibitory concentration (MIC) of value 40 µg/ml and minimum bactericidal concentration (MBC) of value 200 µg/ml. The kinetic time killer curve showed that the bacterial cells die within 4 hours. The transmission electron microscope technique detected Klebsiella pneumoniae strain 3MAG cellular damage after being exposed to Dibutyl phthalate ester instead of its typical cell shape. Bacterial and fungal strains were molecularly identified. The fractionation and purification of the Dibutyl phthalate ester compound were achieved using silica gel column G254 and preparative thin-layer chromatography (TLC). Spectrum data such as UV spectroscopy, LC-mass, and nuclear magnetic resonance (NMR) aided in the structure elucidation of Dibutyl phthalate. The ADME-related physicochemical properties of the obtained compound were predicted using SwissADME web tools and the ProToxii webserver was used to estimate in silico toxicity. While by using inslico molecular docking, Dibutyl phthalate ester interacted with OMPA (7RJJ) through hydrophobic contact with THR475, GLU480, GLN485 and LEU486, Cation-pi interaction with GLU480 and Hydrogen bond with ASN484, THR475 and LEU486 which may lead to disruption of the Klebsiella pneumoniae outer membrane.
Site characterisation based seismic microzonation of the Himalayan region: Implication towards seismic risk assessment for sustainable tunnelling projects
Authors: Ansari Abdullah , Rao KS , Jain AK
Published: December 2022

Infrastructure projects require billions of dollars, but they are destroyed in seconds due to strong ground motion during earthquakes. The disaster mitigation cell of any nation seeks sustainable and resilient infrastructure schemes to reduce the catastrophic effects of any seismic hazard. This study attempted to carry out detailed seismic microzonation of Jammu Region (JR), which is located in the northwestern part of the Himalayas. To accomplish this, seismic hazard analysis and liquefaction assessment are carried out, with a primary focus on site characterisation using geophysical field testing. For the proposed seismic zones, the performance and vulnerability of tunnels are evaluated under various seismic environments. Further, seismic risk of the 345 km long Udhampur Srinagar Baramulla Rail Link (USBRL) Project is assessed for serviceability in post-seismic conditions. The proposed zonation, risk matrices, and route maps will be useful for auditioning existing and proposed tunnelling projects in this area. This will pave the way for earthquake-resistant design of sustainable infrastructure projects in seismically active areas like the Himalayas.
Insecticide resistance status of Aedes aegypti in Southern and Northern Ghana
Authors: Abdulai Anisa , Owusu-Asenso Christopher Mfum , Akosah-Brempong Gabriel , Abdul Rahim Mohammed , Sr Isaac Kwame , Attah Simon Kwaku , Forson Akua Obeng , Weetman David , Afrane Yaw Asare
Published: December 2022

Background
Outbreaks of Aedes-borne arboviral diseases are becoming rampant in Africa. In Ghana, there is no organized arboviral control programme with interventions restricted to mitigate outbreaks. Insecticide application is a crucial part of outbreak responses and future preventative control measures. Thus, knowledge of the resistance status and underlying mechanisms of Aedes populations is required to ensure optimal insecticide choices. The present study assessed the insecticide resistance status of Aedes aegypti populations from southern Ghana (Accra, Tema and Ada Foah) and northern Ghana (Navrongo) respectively.
Methods
Phenotypic resistance was determined with WHO susceptibility tests using Ae. aegypti collected as larvae and reared into adults. Knockdown resistance (kdr) mutations were detected using allele-specific PCR. Synergist assays were performed with piperonyl butoxide (PBO) to investigate the possible involvement of metabolic mechanisms in resistance phenotypes.
Results
Resistance to DDT was moderate to high across sites (11.3–75.8%) and for the pyrethroids, deltamethrin and permethrin, moderate resistance was detected (62.5–88.8%). The 1534C kdr and 1016I kdr alleles were common in all sites (0.65 to 1) and may be on a trajectory toward fixation. In addition, a third kdr mutant, V410L, was detected at lower frequencies (0.03 to 0.31). Pre-exposure to PBO significantly increased the susceptibility of Ae. aegypti to deltamethrin and permethrin (P < 0.001). This indicates that in addition to kdr mutants, metabolic enzymes (monooxygenases) may be involved in the resistance phenotypes observed in the Ae. aegypti populations in these sites.
Conclusion
Insecticide resistance underpinned by multiple mechanisms in Ae. aegypti indicates the need for surveillance to assist in developing appropriate vector control strategies for arboviral disease control in Ghana.
Positive impact of a new compressive garment in patients with genital lymphedema: OLYMPY STUDY
Authors: Mestre Sandrine , Vignes Stéphane , Malloizel-Delaunay Julie , Abba Sarah , Villet Stéphanie , Picolet Astrid , Vicaut Eric , Quéré Isabelle
Published: December 2022

Purpose: Genital lymphedema is a chronic debilitating condition associated with highly impaired health-related quality of life (QoL). This prospective multi-center study evaluated the use of a new compressive garment in patients with secondary and primary genital lymphedema.
Methods: 32 patients prospectively enrolled were advised to wear the compressive garment for 12 weeks (day and night). The primary endpoint was change in patient-reported QoL at 12 weeks via the patient global impression of change (PGI-C) instrument. Secondary outcomes included change in other QoL measures at 12 weeks (VAS, LyQLI and EQ-5D questionnaires), lymphedema severity (Genital Lymphedema Score (GLS)) and physician assessment (Clinical Global Impression- Improvement (CGI-I)). Safety and tolerability were also assessed.
Results: After 12 weeks, improvement was reported in 78.6% of patients (PGI-C). Physician assessment (CGI-I) indicated clinical improvement in 82.8% of patients. Patient assessment of lymphedema symptoms showed a significant decrease in discomfort (p = 0.02) and swelling (p = 0.01). Significant declines in mean global GLS scores (p < 0.0001), and in the proportion of patients reporting heaviness, tightness, swelling or urinary dysfunction (p < 0.05 for all) were also observed. LyQLI scores decreased (indicating improved QoL) in each of the physical, psychosocial (p = 0.05) and practical domains. The compressive garment was well tolerated with high compliance and adverse events (due to swelling or discomfort) led to permanent discontinuation in only 3 patients.
Conclusions: Use of a new genital compression garment over 12 weeks improves QoL and clinical measures in patients with genital lymphedema (NCT04602559; Registration: 10/20/2020).
Impact of trace elements on stability and detoxification of imazethapyr herbicide in water and milk
Authors: Hassan Jalal , Abedini Sepideh , Koohi Mohammad , Hejazy Marzie
Published: December 2022

Imazethapyr is a herbicide belongs to the class of imidazolinone herbicides. Exposure to Imazethapyr (IM) causes residues in body and biological systems. This study was carried out to evaluate the stability of imazethapyr in different chemical & physical conditions such as temperature, ionic strength, pH, presence of metallic ions (Ag+, Cu+2, Na+, Mg+2) in water and milk matrix to predict the factors can diminish Imazethapyr (IM) toxicity in biological systems and environment. Monitoring of imazethapyr was performed using liquid chromatography systems.
The results were shown that temperature and pH and the (Ag+, Na+, Mg+2) ions have no effect on stability degradation or interaction of imazethapyr. Only copper ions (Cu+2) showed selective interaction with imazethapyr and form complex with imazethapyr. This study revealed Cu+2 ions have effect through stabilisation of imazethapyr by complexation. This process can use for detoxification of imazethapyr and Cu+2 in environment and biological systems.
Engineering osmolysis susceptibility in Cupriavidus necator and Escherichia coli for recovery of intracellular products
Authors: Adams Jeremy David , Sander Kyle B. , Criddle Craig S. , Arkin Adam P. , Clark Douglas S.
Published: December 2022

Background:
Intracellular biomacromolecules, such as industrial enzymes and biopolymers, represent an important class of bio-derived products obtained from bacterial hosts. A common key step in the downstream separation of these biomolecules is lysis of the bacterial cell wall to effect release of cytoplasmic contents. Cell lysis is typically achieved either through mechanical disruption or reagent-based methods, which introduce issues of energy demand, material needs, high costs, and scaling problems. Osmolysis, a cell lysis method that relies on hypoosmotic downshock upon resuspension of cells in distilled water, has been applied for bioseparations such as the purification of polyhydroxybutyrate (PHB) from extreme halophiles and protein products from mammalian cells. However, most industrial bacterial strains are non-halotolerant and relatively resistant to hypoosmotic cell lysis.
Results:
To overcome this limitation, we developed two strategies to increase the susceptibility of non-halotolerant hosts to osmolysis using Cupriavidus necator, a strain often used in electromicrobial production, as a prototypical strain. In one strategy, C. necator was evolved to increase its halotolerance from 1.5% to 3.25% (w/v) NaCl through adaptive laboratory evolution, and genes potentially responsible for this phenotypic change were identified by whole genome sequencing. The evolved halotolerant strain experienced an osmolytic efficiency of 47% in distilled water following growth in 3% (w/v) NaCl. In a second strategy, the cells were made susceptible to osmolysis by knocking out the large-conductance mechanosensitive channel (mscL) gene in C. necator. When these strategies were combined by knocking out the mscL gene from the evolved halotolerant strain, greater than 90% osmolytic efficiency was observed upon osmotic downshock. A modified version of this strategy was applied to E. coli BL21 by deleting the mscL and mscS (small-conductance mechanosensitive channel) genes. When grown in medium with 4% NaCl and subsequently resuspended in distilled water, this engineered strain experienced 75% cell lysis, although decreases in cell growth rate due to higher salt concentrations were observed.
Conclusions:
Our strategy is shown to be a simple and effective way to lyse cells for the purification of intracellular biomacromolecules and may be applicable in many bacteria used for bioproduction.
Effect of demographic factors on the outcome of early intervention therapy among the children with autism spectrum disorder in Bangladesh
Authors: Rahman Md. Sahidur , Hoque Mahfara , Hafiz Promugdha
Published: December 2022

Autism Spectrum Disorders is a group of neuro-developmental disorders affecting one in every 100 children worldwide. The cornerstone of ASD management is based on early intervention programs targeted to improve communication, socialization, and behavior skills. This study, conducted at the child development center of a tertiary care hospital in Chattogram city of Bangladesh, aimed to determine the effect of age and other demographic variables on the outcome of early intervention therapy on the improvement of skills of children with ASD. Participants included two groups of children (75 per group) with ASD having different ages - below three years and three to five years. The diagnosis was confirmed using different tools like neurodevelopment assessment form, Bayley scales, M Chat, ICD 10 criteria, and how and why. The intervention was provided on a 1:1 basis and given six subsequent sessions every two months throughout the one-year study period. Statistically significant improvements were observed in different skills in the young age children than in the older group. Almost half of the children (49.3%) from the old age group remained in own agenda stage. On the other hand, all children of young age have significantly improved in early communicator (46.7%), the requester (41.3%), and partner (12%) stages. Moreover, the monthly family income of children posed a significant effect on the outcome of the intervention therapy. The study suggests the initiation of early intervention therapy before age three to get a significant improvement in communication skills of children.
Dendroecological analysis of the remote endemic Nothomyrcia fernandeziana forests of Robinson Crusoe Island in the Southeast Pacific
Authors: Almonacid Pamela , Rojas-Badilla Moisés , LeQuesne Carlos , Muñoz-Salazar Tomás , Christie Duncan A.
Published: December 2022

Juan Fernández Archipelago National Park is located in the Southeast Pacific Ocean ~ 670 km off the coast of Chile. Composed by three islands, encompasses an ecosystem with 208 native plant species of which 64% are endemic. Robinson Crusoe Island (RCI) is the largest and the only with a permanent human population. Among the tree species of RCI, Nothomyrcia fernandeziana is the most abundant and dominant forming part of this fragile forest ecosystem. Despite this, there is very little information regarding its autoecology and conservation. Therefore, the objective of this work was to determine the radial growth patterns of N. fernandeziana and its longevity using dendrochronological methods. For this purpose, cross sections of trees blown down by wind were collected in conjunction with park rangers from five different sites and analyzed using tree-ring methods. Our results confirm that N. fernandeziana is a shade-tolerant species capable of maintaining extremely low initial growth rates under the canopy for several decades. Individual growth series were found to respond rapidly to changes in environmental conditions, revealed by abrupt positive and negative changes in their annual growth. Our data indicate a longevity of up to 450 years, with one N. fernandeziana individual established in the mid XVII century before the arrival of humans to the pristine island ecosystem. The results of this study are essential to strengthen RCI conservation plans considering restoration actions based on the autoecology of this rare endemic tree species, and the control of exotic invasive species that presently threatens the RCI forests ecosystems.
Repetitive transcranial magnetic stimulation can improve the fixation of eyes rather than the fixation preference in children with autism spectrum disorder.
Authors: Tian Li , Ma Shuai , Li Yin , Zhao Mengfei , Xu Chang , Wang Chen , Zhang Xin , Gao Lei
Published: December 2022

Background
Transcranial magnetic stimulation (TMS) has been introduced into the intervention of autism spectrum disorders (ASD) as a possible new therapeutic option for modifying pathological neuroplasticity. However, the stimulating protocols of rTMS for ASD have not been approved unanimously, which affects the clinical popularization and application of rTMS. In addition, there is little research on the improvement of social processing of autistic children by rTMS.
Methods
We explored the clinical efficacy of rTMS and improvement of face processing with the protocol of left high-frequency and right low-frequency on bilateral dorsolateral prefrontal cortex (DLPFC), with a sample of 45 ASD participants aged 2–18.
Results
Our results showed that both the score on the Childhood Autism Rating Scale (CARS) and the fixations on the eyes of the human faces improved by two-session rTMS intervention, except for the eye preference. The mediation analysis indicated the item of “Adaptation to Change” of CARS mediated dominantly the improvement of eye-gaze behavior of ASD participants by rTMS.
Conclusions
Our study revealed the mechanism of rTMS in improving the eye-gaze behavior of the autism population, deepened the understanding of the function of rTMS in treating autistic social disorders, and provided a reference for combined treatment for ASD.
DNA methylation-regulated LINC02587 inhibits ferroptosis and promotes the progression of glioma cells through the CoQ-FSP1 pathway
Authors: Wang Zhengang , Cui Yang , Wang Fanchen , Xu Lixia , Yan Yan , Tong Xiaoguang , Yan Hua
Published: December 2022

Background: Long noncodingRNAs (lncRNAs) are considered key players in the formation and development of tumors.Herein, Gene Expression Profiling Interactive Analysis (GEPIA) was employed as a bioinformatics technology. LINC02587 is differentially expressed in bladder urothelial cancer, glioblastoma, lung adenocarcinoma, lung SCC,melanoma,and other tumortissue and cells. However, its impacton the emergenceof glioma and its mechanism is remaining elusive.
Methods: Some of the in vitro assays employed in this study were the CCK-8 / Annexin-V / Transwell assays, colonyformation, and wound healing, together with Western blot (WB) evaluation. MSP / BSP assays were employed for assessing the CpG island'smethylation status in the LINC02587 promoter. Through transcriptome, ferroptosis-related experiments, and WB evaluation, it was confirmed that LINC02587 is correlated with the regulation of ferroptosisin tumor cells, and CoQ-Fsp1 is one of its regulatory pathways. Moreover, the underlined in-vitro results were further validated by in-vivo studies.
Results: The current study shows that the promoter sequenceof LINC02587 is regulated by methylation. The silencing of LINC02587 can inhibit cellular proliferative, migrative, and invasive properties, and induce ferroptosis within gliomas through the CoQ-FSP1 pathway.
Conclusions: LINC02587 is likelyto be a novel drug target in treating glioma.
4 He Gas in the Temperature-Range 1mK-5K: Thermodynamic Properties from the Quantum Second Virial Coefficient
Authors: Joudeh Bassam R. , Sandouqa A. S. , Al-Obeidat O. T. , Al-Maaitah A. F. , Ghassib H. B.
Published: December 2022

The quantum second virial coefficient Bq of low-dense 4He gas is calculated over the temperature-range 1 mK-5K. This is the first step in determining, according to standard expressions, the thermodynamic properties of the system, namely, the virial equation of state, compressibility factor, total internal energy, specific heat capacity, and entropy. These are compared to previous results wherever available. A large negative Bq is obtained. It is argued that this is intimately related to the formation of small clusters. Based on semi-quantitative analysis, the cluster formation is predicted to occur in the present system at a fairly low temperature ~ 0.049 K to 2.254 K.
Community mental health network care: an evaluative approach in a Brazilian state
Authors: Coelho Vívian Andrade Araújo , Gama Carlos Alberto Pegolo da , Andrade Leonardo Isolani , Silva Mariana Arantes , Guimarães Denise Alves , Guimarães Eliete Albano de Azevedo , Modena Celina Maria
Published: December 2022

In recent decades, public policies of the Unified Health System (SUS) in Brazil have structured a mental health care network (RAPS) based on various community actions and services. This study carried out evaluative research on the implementation of the structure and process dimensions of the RAPS in Minas Gerais (MG), the second most populous state of Brazil, generating indicators that can enhance the strategic management of the SUS in the strengthening the psychosocial care in the state. The application of a multidimensional instrument, previously validated (IMAI-RAPS), in 795 of the 853 municipalities in Minas was carried out between June and August 2020. Regarding the structural dimension, we noticed an adequate implementation of ‘Family Health Strategy (ESF),’ ‘Expanded Family Health Center (NASF),’ and ‘Psychosocial Care Centers (CAPS)’ but a lack of ‘Beds in General Hospitals (LHG),’ ‘Unified Electronic Medical Records’ and ‘Mental Health Training Activities for Professionals’. In the process dimension, adequate implementation of actions such as ‘Multidisciplinary and Joint Care,’ ‘Assistance to Common Mental Disorders by Primary Health Care,’ ‘Management of Psychiatric Crises in CAPS,’ ‘Offer of Health Promotion Actions,’ and ‘Discussion of Cases by Mental Health Teams’ point to a form of work consistent with the guidelines. However, we detected difficulties in the implementation of ‘Psychosocial Rehabilitation Actions,’ ‘Productive Inclusion,’ ‘User Protagonism,’ ‘Network Integration,’ and practical activities for the effectiveness of collaborative care. We found a better implementation of RAPS in more populous, demographically dense, and socioeconomically developed cities, which shows the importance of regional sharing of services that are not possible for small cities. The evaluation practices of RAPS are scarce throughout the Brazilian territory, a fact also found in MG, highlighting the need for its expansion not only in the scientific sphere but also in the daily life of the various levels of management.
A Coordination Control Algorithm as an Automobile Application for Wheel Torque of Parallel Hybrid Vehicles in Industry 4.0
Authors: Kaur Mandeep , Kharat Vilas , Patil Rajkumar
Published: December 2022

In Industry 4.0, everything is automated for utilizing the advantages of AI and Internet of Things (IoT). The automation is required for robotics based operations in the industries. The automobile industries are attempting to utilize the computational algorithms for improving the performance of the automobiles and for improving the performance of production lines. Innovative methods are demanded to resolve the automation problems in industry 4.0. In this paper a coordination control algorithm is proposed for wheel torque for parallel hybrid vehicles. Based on the fuzzy adaptive PID principle (proportional integral derivative), the wheel torque of a parallel hybrid vehicle is coordinated and controlled in the proposed work. To overcome the existing coordination problems of wheel torque, a new coordination control algorithm is designed for parallel hybrid vehicles. In wheel torque drive control, the feedforward and feedback joint control method of the wheel drive torque is utilized. The wheel braking torque control algorithm is used with the fuzzy PID controller to obtain the wheel braking torque; the engine output torque is compensated based on the PID control method of wheel angular speed difference, and a dynamic coordination controller is designed to keep an eye on the performance of the vehicle. The compensatory torque is provided by the motor, and the difference between the actual and intended angular velocity is changed in real time to provide dynamic coordination of the wheel under mode switching control. To assess the performance of the proposed control algorithm, the results are compared to those of the present system. As a result of implementing the proposed algorithm, the total output torque of a parallel hybrid vehicle in the industry 4.0 context can be reduced to 0.08 seconds, the accuracy of torque control can be increased by 11.1% over the existing system, the accuracy of the speed at which the vehicle tracks its speed can be increased by 8.0% over the existing system, and the vehicle dynamics can be improved by 4.4% during the switching process.
Evaluation of Sustainability Awareness in Uzbekistan
Authors: Veckalne Regina , Us Yana , Gerulaitiene Neringa
Published: December 2022

In the frame of this article, the authors maintained that natural resources and environmental conditions are the basis of material production and the life of the population. Systematization of the scientific sources on sustainable development indicated that the state of the environment and the quality level of resource use, protection, and reproduction largely determine the rates of economic growth and production efficiency. The relevance of this scientific problem decision is that at the present stage of economic development, consideration of the ecological and social factors when making decisions on sustainable development is becoming especially significant. One of the factors affecting social behaviors when it comes to sustainable decisions is awareness of the importance of sustainability. The primary purpose of this research is to measure sustainability awareness in Uzbekistan. A survey of 440 Uzbek citizens was conducted to fulfill the research aim. The survey results were analyzed using SmartPLS software. The paper presents the results of an empirical analysis, which showed that environmental awareness is positively and significantly connected with energy conservation. At the same time, the authors found that energy conservation is positively and significantly connected with sustainability issues. In line with the above findings, the obtained results allowed stating that sustainability issues are positively and significantly connected with sustainability awareness. The authors concluded that raising awareness among the population could positively effect the sustainability issues being solved in the region. The scientific novelty of this research could be described in the fact that, to the best of the authors’ knowledge, no such research has been previously conducted.
Identification of Preferred Representational Sensory System in Neuro-Linguistic Programming
Authors: Birknerova Zuzana , Misko David , Ondrijova Ivana , Tomkova Anna , Cema Vladimir , Cigarska Barbara Nicole
Published: December 2022

The paper highlights the scientific debate on the Neurolinguistic programming (NLP) issue. NLP is a collection of approaches, communication tools, techniques, and perspectives that determine how individuals think and communicate through language. NLP is used to recognize and modify patterns of human behavior. The sensory representational system, or the method for recognizing representational systems, which is made up of five main senses, influences this process. Systematization literary sources and approaches to this issue indicate that three sensory representational systems exist in the NLP approach: visual, auditory, and kinesthetic (VAK), and that the individual’s preferred representational sensory system could explain manifested behavior and characteristics in the managerial and marketing sphere. The central purpose of the research and the significance of choice made about this area of interest is to determine each individual’s preferred representational sensory system (VAK) utilizing the original PRSS-VAK methodology. The methodological research tool was the PRSS-VAK methodology which contains nine statements, which are assessed on a scale from 1 (the least describes me) to 4 (the most describes me). The PRSS-VAK methodology would help to comprehend patterns of an individual’s behavior and allied cognitive or emotional processes. EFA (Exploratory Factor Analysis) with Varimax rotation was used to verify the methodology on a sample of 214 respondents from the Slovak Republic, and CFA (Confirmatory Factor Analysis) was used to validate the structure on a sample of 268 respondents from the Slovak Republic. This research empirically and theoretically confirms that one of the preferred representational sensory systems may be dominant. However, this may change regarding the current situation (stimulus, impulse). The research results could be beneficial as a springboard not only for researchers concerning this issue. It also indicates that quantitative research does not determine exactly to which category (visual, auditory, or kinesthetic) a certain individual belongs. Using the identification of a preferred representational sensory system could help to facilitate both management and marketing communications.
A Comparison of Two Firmness-testing Machines for Measuring Blueberry Firmness and Size
Authors: Luby Claire H. , Doane Sarah , Mackey Ted , Yang Wei Q.
Published: December 2022

Firmness is an important fruit quality trait in northern highbush blueberry (Vaccinium corymbosum). Many researchers, growers, and packers rely on machines for measuring firmness right after harvest and during postharvest cold storage of fresh fruit. In this study, we compared two machines that use compression firmness measurements to determine a force-deformation value. The first firmness-testing machine has been in use for the past 30 years by blueberry (Vaccinium) researchers and packers worldwide. The second has been on the market for the past 5 years. We compared fruit firmness and size measurements for several commercial cultivars and breeding accessions of northern highbush blueberry by both machines at harvest and 2 weeks postharvest. In general, we found there were slight differences in fruit firmness and size measurements between the two machines, but these measurements were generally consistent across the machines. Our study suggests that, in general, one machine can predict the measurements taken on the other machine.
Nutrient Use and Management Practices on United States Golf Courses
Authors: Shaddox Travis W. , Unruh J. Bryan , Johnson Mark E. , Brown Clark D. , Stacey Greg
Published: December 2022

Nutrient use on United States golf courses increases management costs and has the potential to influence ecosystems. Therefore, it is critical to assess nutrient use and management practices to develop and teach best management practices. The objectives of this survey were to measure nutrient use and management practices on United States golf courses in 2021, and to determine if changes occurred since 2006. A survey was developed and distributed via e-mail to 14,033 United States golf facilities, with 1444 responding. From 2006 to 2021, the total projected nitrogen (N), available phosphorus (P2O5), and soluble potash (K2O) applied declined by 41%, 59%, and 54%, to 54,376, 13,761, and 41,386 tons, respectively. These reductions were attributed to course closures, reduced fertilized acres, reduced application rates, and nutrient use restrictions. The percentage of facilities that did not apply P2O5 increased to 21%, which is likely a result of P2O5 application restrictions. Soil testing was associated with greater application rates of N, P2O5, and K2O. Returning clippings, using precision fertilizer applications, reducing turfgrass acreage, and considering N release from soil organic matter were associated with reduced application rates of P2O5. Golf course superintendents have contributed to nationwide reductions in N, P2O5, and K2O, as evidenced by the reduction in fertilized acres and the reduction in nutrient use rates from 2006 to 2021.
United States Consumers’ Landscaping Expenditures: A Case of Garden and Lawn Care Services and Equipment
Authors: Cui Manlin , Yue Chengyan , Zhao Li , Watkins Eric
Published: December 2022

The economic downturns of 2007–09 and the COVID-19 pandemic affected most industries in the United States, including landscape services and equipment sales, and provoked both short-term disruptions and long-term changes. To understand how the landscaping industry has responded, we investigated patterns of consumer expenditures on landscape services and equipment from 2009 through 2021 using a representative sample of 76,895 US households. We categorized US households as detached single-family residents and townhouse residents to more fully articulate the factors that turned potential consumers into purchasers and the factors that affected purchasers’ expenditures. We used a double-hurdle model to identify key factors that drive consumer demand for landscape services and equipment over time, including social-demographics, geographic characteristics, housing conditions, year and seasonal trends, and the COVID-19 pandemic. We found that during the studied period, the demand for landscape services declined in terms of both the percentage of consumers purchasing the services and the purchasers’ average expenditures, while the demand for do-it-yourself (DIY) equipment remained relatively unchanged. In response to the COVID-19 pandemic, the percentage of consumers who purchased landscape services increased, while the expenditures on landscape services decreased in 2020 and then began to rebound in 2021, but not enough to reverse the overall downward trend. In contrast, purchases of DIY equipment were relatively stable in response to the COVID-19 pandemic and mainly relied on current consumers.
Space Lexicalization in English Language Education Students’ Narrative Writing
Authors: Khairunisa Naima , Basthomi Yazid , Anugerahwati Mirjam
Published: December 2022

This article discusses how Indonesian English Language Education Students (ELES) apply space lexicalization in narrative composition. This study also discusses how they understand the concept of space lexicalization. Fifteen master’s degree students in English Language Education are the subjects of this study. A convergent parallel mixed-method research design was utilized in this study. The data of this study are a set of tests on space lexicalization and a writing test. The test on space lexicalization is used to investigate the participants’ understanding of space lexicalization’s concept, while the writing test is utilized to investigate how they apply space lexicalization in narrative writing. The results show that the subjects understand the concept of space lexicalization and they can apply space lexicalization in narrative writing. This shows that Indonesian ELES has acquired space lexicalization in narrative writing, although some of them produce grammatical mistakes and incorrect combinations of spatial words.
ISLAMOPHOBIA IN INDO-PACIFIC COUNTRIES AND THE ROLES OF REGIONAL ORGANIZATION
Authors:   Associate Professor, Faculty of Social and Political Sciences, Universitas Kristen Indonesia, Jakarta., Damayanti Angel , Robertua Siahaan Verdinand ,   Assistant Professor, Faculty of Social and Political Sciences, Universitas Kristen Indonesia, Jakarta., Mulyaman Darynaufal ,   Lecturer, Faculty of Social and Political Sciences, Universitas Kristen Indonesia, Jakarta.
Published: December 2022

Many scholars accused that anti-Islam sentiment in many countries came after the 2001 Al-Qaeda attack. This study proves that such a phenomenon has become an issue in Indo-Pacific countries long before the attack. The article explains the root causes and impacts of Islamophobia in India, Myanmar, Thailand, and South Korea. It suggests that the regional organization should play a role in addressing this problem. The concepts of Islamophobia and national and human security are used to examine the issue. Qualitative methodology in case studies was used, with data collection from focused group discussions and literature reviews. The results show that the causes of Islamophobia range from the historical problem of colonialism, religious conflicts between majority and minority groups within a socio-political framework, separatism issues, and the medias role that stigmatizes Muslims. The Islamic-based terrorist attack in 2001 is merely a justification for violating Muslims rights in Indo-Pacific countries. This condition threatens the regions stability and leads to serious human rights violations. Therefore, ASEAN, as a regional organization in Southeast Asia and partnering with Indo-Pacific countries, must address the issue while resolving its internal challenges.
IMPACTS OFTHE DEGRADATION OF THE FOREST FORMATIONS OF THE GUESSELBODI FOREST ON THE RIPARIAN POPULATIONS
Authors:   Universite Boubakar BA De Tillaberi, Faculte Des Sciences Agronomiques. BP : 175, Tillaberi (NIGER)., Abdoul-Salam Koroney , Issaka Mayaki Souley ,   Universite Boubakar BA De Tillaberi, Faculte Des Sciences Agronomiques. BP : 175, Tillaberi (NIGER)., Illiassou Naroua ,   Universite Boubakar BA De Tillaberi, Faculte Des Sciences Agronomiques. BP : 175, Tillaberi (NIGER)., Hamani Farouk Garba ,   Universite Boubakar BA De Tillaberi, Faculte Des Sciences Agronomiques. BP : 175, Tillaberi (NIGER).
Published: December 2022

The Sahel, like most arid and semi-arid environments, is clearly a geographical area where the question of the degradation of natural resources is of concern. In this context, we have initiated a study on the socio-economic and cultural impact of the degradation of dry forest formations on the local population of Guesselbodi in Niger. The methodology mainly consisted of surveys using a participatory approach with the local population and forestry agents of the municipality of Kollo. This approach has made it possible (i) to identify the climatic risks that have a greater influence on the forest, (ii) to determine the socio-economic and cultural impacts of the effects of the degradation of dry forest formations on the local populations. The results of these investigations were able to reveal the various impacts that the local population faces such as the reduction of fauna and flora, land grabbing attempts, the dumping of incinerated toxic waste, etc. As for the adaptation strategies put in place, they are very unfortunately inadequate and contribute intensely to the degradation of the forest. The study also made it possible to make a comparison of both plant and animal species that existed in the past and today. Thus, it has been identified that 30 plant species out of the 61 initially present in the forest and 20 animal species out of the 40 initially present. This drastic decrease in the fauna and flora of the Guesselbodi forest is due not only to the destruction of their habitat but also to anthropogenic pressure.
AİLE HUKUKUNDAN DOĞAN YÜKÜMLÜLÜKLERİN ÖNEMLİ ÖLÇÜDE YERİNE GETİRİLMEMESİ NEDENİYLE MİRASÇILIKTAN ÇIKARMA
Authors: ŞAHİN CANER Ayşenur
Published: December 2022

Mirasçılıktan çıkarma, mirasbırakana saklı paylı mirasçısını saklı payından tamamen veya kısmen mahrum etme imkânı veren ölüme bağlı tasarruftur. Türk/İsviçre Hukuk sistemlerinde mirasçılıktan çıkarmanın iki türü bulunmaktadır ki bunlardan ilki koruyucu mirasçılıktan çıkarma, ikincisi ise cezai mirasçılıktan çıkarmadır. Aile hukukundan doğan yükümlülüklerin önemli ölçüde ihlal edilmesi nedeniyle mirasçılıktan çıkarılma için birtakım şartların gerçekleşmesi gerekir ki bu şartlar, aile hukukundan doğan yükümlülüklerin önemli ölçüde ihlali, bu ihlalin hukuka aykırı olması ve saklı paylı mirasçının kusurlu olmasıdır. Aile hukukundan doğan yükümlülüklerin önemli ölçüde yerine getirilmediğinden bahsedebilmek için mirasçının fiilinin objektif olarak aile hukukundan doğan yükümlülükleri ihlal etmesi dışında bu fiil nedeniyle sübjektif olarak da aile bağlarının kesilmesi şarttır. 4721 sayılı Türk Medeni Kanunu m.510 gereğince mirasçı, mirasbırakana veya mirasbırakanın aile üyelerine karşı aile hukukundan doğan yükümlülükleri önemli ölçüde yerine getirmemişse mirasçılıktan çıkarılabilir. Mirasçılıktan çıkarma için çıkarma sebebinin ölüme bağlı tasarrufta açıkça gösterilmesi şart olduğundan, tasarrufta belirtilen genel ve soyut sebep mirasçılıktan çıkarma bakımından yeterli değildir.
Impact of the COVID-19 outbreak on stock returns of Indian healthcare and tourism sectors
Authors: Joshipura Mayank , Lamba Ashu
Published: December 2022

The rapid spread of the novel coronavirus pandemic (COVID-19) has adversely impacted global economies and stock markets. This study employs an event study methodology to assess the impact of COVID-19 on stock returns in the healthcare (66 stocks) and tourism (39 stocks) sectors in Indian markets surrounding two events: a) the first COVID-19 case reported in India and b) the announcement of a nationwide lockdown. The findings indicate that investors’ reactions to both events were distinct and asymmetric in healthcare and tourism sectors. The tourism sector stocks react more negatively to the second event than the first, with –2.46% vs. –0.59% event day abnormal returns, respectively. The corresponding figures for healthcare sector stocks are –0.68% and –0.16%, respectively. As expected, pandemic events had a minor negative impact on the healthcare sector. Surprisingly, the tourism industry did not react negatively to the first event. Investors in the tourism industry underreacted to the first reported case; they could not predict the potential consequences and then overreacted to the lockdown announcement. The findings support the behavioral finance theory of underreaction and overreaction, particularly in stressful situations. The study has implications for investors and money managers looking for profitable investment opportunities due to temporary dislocations in stock prices caused by investors’ irrational reactions to certain black swan events.
Social Conditions оf the Criminalization of Collaborative Activities іn Ukraine
Authors: Sorokin Artem
Published: December 2022

The application of criminal law measures or criminal liability for violation of legal norms is an exclusive and particularly strict measure of state coercion, therefore the question of the feasibility and correctness of introducing a new article into the criminal law is extremely urgent. The purpose of the article is to clarify the essence and grounds of criminalization of collaborative activities in Ukraine. The methodology consists of terminological, system-structural methods, the method of analysis and synthesis, and logical-deductive. It is noted that social conditionality is the state’s response to society’s need to introduce a new level and quality of guarantees of legality. The main task of social conditionality is the study of the circumstances that affect the creation of norms and institutions of criminal legislation and their effectiveness. It has been proven that the criminalization of collaborative activity is conditioned by a number of reasons (the existence of socially dangerous behavior that requires a criminal law prohibition, the relative prevalence of socially dangerous acts, a change in ideas about the nature and degree of social danger of an act, a change in the generally recognized moral assessment of the relevant act, the insufficiency of available means, other than criminal law, to effectively counteract these acts, the need for a criminal law guarantee of rights and freedoms), which are objective in nature and in together, they determine the need to introduce criminal liability for such an act. Prospects for the author’s future research are the study of the practices of foreign states regarding the criminalization of collaborative activities.
The Concept оf the Legal Collisions
Authors: Priyma Serhiy , Erofeeva Maria
Published: December 2022

The article considers the conflict of law as one of the drowbacks of the law. It has been established that legal conflicts are an objectively existing, natural defect of law that arises due to various objective and subjective circumstances.
To realize the purpose of the article, the following main features of legal conflicts were singled out: 1) their essence is that they are an excess of legal regulation. At the same time, three conditions are necessary for the emergence of a legal conflict: two or more valid legal norms; regulate the same social relationship; such regulation is carried out in different ways. 2) They exist solely between legal norms, as the rules of behavior of the relevant persons; 3) arise at the stage of law-making, but it is revealed at the stage of law application and is resolved with the help of appropriate means.
The article singles out the following reasons for the emergence and existence of the legal conflicts: 1) violation of rules and failure the requirements of the law-making technique; 2) insufficient professional qualification of authorized subjects of law creation; 3) unclear demarcation of the law-making competence of authorized subjects; 4) a large number of legal norms, due to which both difficulties in the work of norm design bodies and errors in legal qualification by the law enforcement and law realization bodies are possible; 5) the simultaneous action of the norms of different historical eras - Soviet and Ukrainian - in Ukrainian legislation.
The article also considers the areas in which legal conflicts arise and proves that they have the most harmful effect on the realization of the legal certainty principle, because the ambiguity and contradiction of legal norms, which are the violation of this principle and are the basis for the emergence of legal conflicts, and therefore failure the requirements of this principle is one of the methods to reduce the conflict of law.
Legal conflicts are defined as a type of legal defect that arises due to the implementation of excessive legal regulation of social life, which leads to a contradiction between the valid legal norms in the regulation of the same social relations.
Mass actions in the context of pre-election campaigning
Authors: В. Є. Шеверєва
Published: December 2022

The article considers the mass actions as a manifestation of political activity and form of campaigning. Analyzes of these forms of pre-election campaigning  is conducted that can be attributed to mass actions, such as: meetings, marches, demonstrations, pickets, concerts, performances, sporting events, etc., in terms of the current legislation and the draft Law on Freedom of Assembly, with the participation of candidates for election. Own recommendations on the regulation of mass actions as a form of campaigning in the future law on such events have been proposed.
Executive power and governance: value concepts
Authors: Є. А. Гетьман
Published: December 2022

The paper analyzed the basic scientifi c approaches to separation of concepts such as «executive» and «governance». Investigated the relationship between these concepts in the former Soviet Union and Europe.
Current problems of state policy support to the ukrainians abroad
Authors: О. Г. Кушніренко
Published: December 2022

Review some of the public policy support relationships with foreign Ukrainians, ways and measures of government and community organizations to further enhance cooperation with foreign Ukrainians.