bluuebunny/crossref_metadata_2025 huggingface dataset:
Chaotic Dynamics of the Interface between Dielectric Liquids at the Regime of Stabilized Kelvin-Helmholtz Instability by a Tangential Electric Field
Authors: Kochurin Evgeny A. , Zubarev Nikolay M.
Published: March 2021

The nonlinear dynamics of the interface between two immiscible dielectric liquids at the regime of suppressed Kelvin-Helmholtz instability by external horizontal electric field is studied theoretically. The initial equations of the fluids motion are reduced to a single weakly nonlinear integro-differential equation that describes the interaction of solitary waves (rational solitons) propagating along the interface. The dynamics of two interacting solitons is regular and integrable; they can combine into a stable wave packet (breather). It is shown that the interaction of three solitons becomes complex and, for a wide rang of initial conditions, chaotic. The numerically obtained Poincaré sections demonstrate the destruction of toroidal trajectories in the phase space during the transition of the system to a chaotic regime of fluid motion. Such a behaviour is consistent with the Kolmogorov-Arnold-Moser theory describing quasi-periodic chaotic motion in Hamiltonian systems. At the developed chaotic state, the system fast loses the information on its initial state; the corresponding estimate for Lyapunov exponent is obtained. From the physical point of view, the chaotic behavior of the system is related with structural instability of the soliton triplet. The triplet can decay into a solitary wave and stable breather consisting of two interacting solitons.
Correction: Shelat, K.J.; et al. Overall Nutritional and Sensory Profile of Different Species of Australian Wattle Seeds (Acacia spp.): Potential Food Sources in the Arid and Semi-Arid Regions. Foods 2019, 8, 482
Authors: Shelat Kinnari J. , Adiamo Oladipupo Q. , Mantilla Sandra M. Olarte , Smyth Heather E. , Tinggi Ujang , Hickey Sarah , Rühmann Broder , Sieber Volker , Sultanbawa Yasmina
Published: March 2021

The authors wish to make the following correction to the paper [1]:The title of Table 3 “Amino acid (g/100 g dry weight) profile of four different species of wattle seeds” should be changed to “Amino acid (mol% dry weight) profile of four different species of wattle seeds” [...]
Comparison of the Chemical and Technological Characteristics of Wholemeal Flours Obtained from Amaranth (Amaranthus sp.), Quinoa (Chenopodium quinoa) and Buckwheat (Fagopyrum sp.) Seeds
Authors: De Bock Phara , Daelemans Lori , Selis Lotte , Raes Katleen , Vermeir Pieter , Eeckhout Mia , Van Bockstaele Filip
Published: March 2021

A sound fundamental knowledge of the seed and flour characteristics of pseudocereals is crucial to be able to promote their industrial use. As a first step towards a more efficient and successful application, this study focuses on the seed characteristics, chemical composition and technological properties of commercially available pseudocereals (amaranth, quinoa, buckwheat). The levels of starch, fat, dietary fiber and minerals were comparable for amaranth and quinoa seeds but the protein content is higher in amaranth. Due to the high amount of starch, buckwheat seeds are characterised by the lowest amounts of fat, dietary fibre and minerals. Its protein content ranged between that of amaranth and quinoa. Buckwheat seeds were larger but easily reduced in size. The lipid fraction of the pseudocereals mostly contained unsaturated fatty acids, with the highest prevalence of linoleic and oleic acid. Palmitic acid is the most abundant unsaturated fatty acid. Moreover, high levels of P, K and Mg were found in these pseudocereals. The highest phenolic content was found in buckwheat. Amaranth WMF (wholemeal flour) had a high swelling power but low shear stability. The pasting profile strongly varied among the different quinoa WMFs. Buckwheat WMFs showed high shear stability and rate of retrogradation.
Rapid, Effective, and Versatile Extraction of Gluten in Food with Application on Different Immunological Methods
Authors: Segura Verónica , Díaz Jacobo , Ruiz-Carnicer Ángela , Muñoz-Suano Alba , Carrillo-Carrión Carolina , Sousa Carolina , Cebolla Ángel , Comino Isabel
Published: March 2021

One of the main concerns in gluten analysis is to achieve efficient extraction of gluten proteins. Conventional ethanol-based extraction solutions are inefficient and, because of this, it is necessary to use reducing agents or acids for proper solubilization. The extraction recommended by CODEX Standard 118-1979 (revised 2008) utilizes Cocktail solution (patent WO 02/092633 A1). However, it is harmful with a disgusting odor and is not compatible with some immunological techniques. Here, the versatility and extraction capacity of a new Universal Gluten Extraction Solution (UGES) (patent ES 2 392 412 A1) were evaluated using different methodological conditions, food matrices, and various immunological methods. UGES includes safer compounds for both the user and the environment, and it displayed similar extraction efficiency to that of the extraction method recommended for sandwich enzyme-linked immunosorbent assay (ELISA). The extraction time was significantly reduced from 100 to 40 min, depending on the type of the sample. Furthermore, unlike the currently used solution, UGES is compatible with competitive ELISA.
Revalorization of Coffee Husk: Modeling and Optimizing the Green Sustainable Extraction of Phenolic Compounds
Authors: Rebollo-Hernanz Miguel , Cañas Silvia , Taladrid Diego , Benítez Vanesa , Bartolomé Begoña , Aguilera Yolanda , Martín-Cabrejas María A.
Published: March 2021

This study aimed to model and optimize a green sustainable extraction method of phenolic compounds from the coffee husk. Response surface methodology (RSM) and artificial neural networks (ANNs) were used to model the impact of extraction variables (temperature, time, acidity, and solid-to-liquid ratio) on the recovery of phenolic compounds. All responses were fitted to the RSM and ANN model, which revealed high estimation capabilities. The main factors affecting phenolic extraction were temperature, followed by solid-to-liquid ratio, and acidity. The optimal extraction conditions were 100 °C, 90 min, 0% citric acid, and 0.02 g coffee husk mL−1. Under these conditions, experimental values for total phenolic compounds, flavonoids, flavanols, proanthocyanidins, phenolic acids, o-diphenols, and in vitro antioxidant capacity matched with predicted ones, therefore, validating the model. The presence of chlorogenic, protocatechuic, caffeic, and gallic acids and kaemferol-3-O-galactoside was confirmed by UPLC-ESI-MS/MS. The phenolic aqueous extracts from the coffee husk could be used as sustainable food ingredients and nutraceutical products.
Adjustment of Whey:Casein Ratio from 20:80 to 60:40 in Milk Formulation Affects Food Intake and Brainstem and Hypothalamic Neuronal Activation and Gene Expression in Laboratory Mice
Authors: Wood Erin L. , Christian David G. , Arafat Mohammed , McColl Laura K. , Prosser Colin G. , Carpenter Elizabeth A. , Levine Allen S. , Klockars Anica , Olszewski Pawel K.
Published: March 2021

Adjustment of protein content in milk formulations modifies protein and energy levels, ensures amino acid intake and affects satiety. The shift from the natural whey:casein ratio of ~20:80 in animal milk is oftentimes done to reflect the 60:40 ratio of human milk. Studies show that 20:80 versus 60:40 whey:casein milks differently affect glucose metabolism and hormone release; these data parallel animal model findings. It is unknown whether the adjustment from the 20:80 to 60:40 ratio affects appetite and brain processes related to food intake. In this set of studies, we focused on the impact of the 20:80 vs. 60:40 whey:casein content in milk on food intake and feeding-related brain processes in the adult organism. By utilising laboratory mice, we found that the 20:80 whey:casein milk formulation was consumed less avidly and was less preferred than the 60:40 formulation in short-term choice and no-choice feeding paradigms. The relative PCR analyses in the hypothalamus and brain stem revealed that the 20:80 whey:casein milk intake upregulated genes involved in early termination of feeding and in an interplay between reward and satiety, such as melanocortin 3 receptor (MC3R), oxytocin (OXT), proopiomelanocortin (POMC) and glucagon-like peptide-1 receptor (GLP1R). The 20:80 versus 60:40 whey:casein formulation intake differently affected brain neuronal activation (assessed through c-Fos, an immediate-early gene product) in the nucleus of the solitary tract, area postrema, ventromedial hypothalamic nucleus and supraoptic nucleus. We conclude that the shift from the 20:80 to 60:40 whey:casein ratio in milk affects short-term feeding and relevant brain processes.
Marinated Sea Bream Fillets Enriched with Lactiplantibacillus plantarum and Bifidobacterium animalis subsp. lactis: Brine Optimization and Product Design
Authors: Speranza Barbara , Bevilacqua Antonio , Racioppo Angela , Campaniello Daniela , Sinigaglia Milena , Corbo Maria Rosaria
Published: March 2021

This study aimed to design marinated sea bream fillets, inoculated with either Lactiplantibacillus plantarum (strains 11, 68, 69) or Bifidobacterium animalis subsp. lactis DSM 10140. In the first step, the optimization of brine composition was performed through a centroid; the factors of the design were citric acid, vinegar, and salt. As a result of optimization, the optimal composition of brine was set to 0.75% citric acid, 55% vinegar, and 3% NaCl. In the second step, sea bream fillets were inoculated with L. plantarum strain 69 and B. animalis subsp. lactis, marinated and then packed in a conditioning solution (oil or diluted brine); the samples were stored at 4 °C for 21 days. The viability of the strains and sensory scores were assessed. The bacteria retained a high viability throughout storage (21 days); however, the sensory scores were at their highest level for 4 days. In particular, sensory assessment suggested a preference for a conditioning solution with oil, rather than with a diluted brine. In addition, a slightly higher preference was found for B. animalis subsp. lactis.
Rheological and Nutritional Assessment of Dysphagia—Oriented New Food Preparations
Authors: Cuomo Francesca , Angelicola Martina , De Arcangelis Elisa , Lopez Francesco , Messia Maria Cristina , Marconi Emanuele
Published: March 2021

Dysphagia that involves difficulty swallowing food and liquids is a symptom of different diseases. In some cases, patients who experience this symptom should be fed with modified consistency foods. Dysphagia is often accompanied by malnutrition and dehydration and an interesting approach to dealing with these conditions is to provide patients with nutrient-rich foods. In this study, two new food formulations for dysphagia patients are proposed: a cereal-based protein meal and a vegetable cream. The nutritional and rheological characteristics of the two innovative preparations were assessed and compared with those of commercial products. The proposed protein meal formulations meet the criteria for the “high protein” claim and the vegetable cream meets those of the “source of fiber” claim. The rheological investigation revealed that the flow properties of the innovative formulations were comparable to those of the commercial ones. Based on these preliminary outcomes, this investigation represents an interesting perspective potentially valuable to enlarge the offer of possibilities for people suffering from swallowing disorders.
A Rational Approach for the Production of Highly Soluble and Functional Sunflower Protein Hydrolysates
Authors: Beaubier Sophie , Albe-Slabi Sara , Aymes Arnaud , Bianeis Marine , Galet Olivier , Kapel Romain
Published: March 2021

Exploitation of plant proteins as an alternative to animal proteins currently presents an important challenge for food industries. In this contribution, total sunflower protein isolate from cold press meal was used as a starting material for the generation of highly soluble and functional hydrolysates that could be used in various food formulations. To do this, a rational and complete approach of controlled hydrolysis was implemented using the individual Alcalase and Prolyve enzymes. The method of stopping the hydrolysis reaction was also evaluated. The influence of operating conditions on hydrolysis kinetics and enzymatic mechanism was studied to identify the appropriate hydrolysis conditions. The gain of the solubility was then analyzed and compared to that of the initial proteins. Finally, the emulsifying and foaming properties (capacities and stabilities) of the resulting hydrolysates were also assessed. As a result, controlled enzymatic proteolysis significantly improved the sunflower protein solubility at neutral pH (twofold increase) and generated highly soluble hydrolysates. The limited proteolysis also maintained the good foam capacities and allowed an improvement in the initial foam stabilities and emulsifying capacities and stabilities of sunflower proteins. This contribution can greatly increase the value of sunflower meal and help in the development of sunflower protein products in the future.
How Perceptions of Trust, Risk, Tap Water Quality, and Salience Characterize Drinking Water Choices
Authors: Grupper Madeline A. , Schreiber Madeline E. , Sorice Michael G.
Published: March 2021

Provision of safe drinking water by water utilities is challenged by disturbances to water quality that have become increasingly frequent due to global changes and anthropogenic impacts. Many water utilities are turning to adaptable and flexible strategies to allow for resilient management of drinking water supplies. The success of resilience-based management depends on, and is enabled by, positive relationships with the public. To understand how relationships between managers and communities spill over to in-home drinking water behavior, we examined the role of trust, risk perceptions, salience of drinking water, and water quality evaluations in the choice of in-home drinking water sources for a population in Roanoke Virginia. Using survey data, our study characterized patterns of in-home drinking water behavior and explored related perceptions to determine if residents’ perceptions of their water and the municipal water utility could be intuited from this behavior. We characterized drinking water behavior using a hierarchical cluster analysis and highlighted the importance of studying a range of drinking water patterns. Through analyses of variance, we found that people who drink more tap water have higher trust in their water managers, evaluate water quality more favorably, have lower risk perceptions, and pay less attention to changes in their tap water. Utility managers may gauge information about aspects of their relationships with communities by examining drinking water behavior, which can be used to inform their future interactions with the public, with the goal of increasing resilience and adaptability to external water supply threats.
Circulating Mediators of Apoptosis and Inflammation in Aging; Physical Exercise Intervention
Authors: Morawin Barbara , Tylutka Anna , Chmielowiec Jolanta , Zembron-Lacny Agnieszka
Published: March 2021

Sarcopenia is an age-related loss of skeletal muscle mass caused by many cellular mechanisms and also by lifestyle factors such as low daily physical activity. In addition, it has been shown that sarcopenia may be associated with inflammation and cognitive impairment in old age. Regular exercise is key in reducing inflammation and preventing sarcopenia and diseases related to cognitive impairment. The study was designed to assess the impact of exercise training on circulating apoptotic and inflammatory markers of sarcopenia in older adults. Eighty older adults aged 70.5 ± 5.8 years were randomized to the physically active group who participated in a 10-month Tai-Chi training session (TC, n = 40) and the control group who participated in health education sessions (HE, n = 40). Tai-Chi training caused a significant decrease in fat mass (FM) by 3.02 ± 3.99%, but an increase in appendicular skeletal muscle mass index (ASMI) by 1.76 ± 3.17% and gait speed by 9.07 ± 11.45%. Tai-Chi training elevated the plasma levels of C-reactive protein (CRP), tumor necrosis factor (TNFα), and tumor necrosis receptor factor II (TNFRII), and decreased caspases 8 and 9. Despite the increase in TNFα, apoptosis was not initiated, i.e., the cell-free DNA level did not change in the TC group. The study demonstrated that Tai-Chi training significantly reduced the symptoms of sarcopenia through the changes in body composition and physical performance, and improvements in cytokine-related mechanisms of apoptosis.
Variability of Urinary Creatinine in Healthy Individuals
Authors: Sallsten Gerd , Barregard Lars
Published: March 2021

Many urinary biomarkers are adjusted for dilution using creatinine or specific gravity. The aim was to evaluate the variability of creatinine excretion, in 24 h and spot samples, and to describe an openly available variability biobank. Urine and blood samples were collected from 60 healthy non-smoking adults, 29 men and 31 women. All urine was collected at six time points during two 24 h periods. Blood samples were also collected twice and stored frozen. Analyses of creatinine in urine was performed in fresh urine using an enzymatic method. For creatinine in urine, the intra-class correlation (ICC) was calculated for 24 h urine and spot samples. Diurnal variability was examined, as well as association with urinary flow rate. The creatinine excretion rate was lowest in overnight samples and relatively constant in the other five samples. The creatinine excretion rate in each individual was positively correlated with urinary flow rate. The creatinine concentration was highest in the overnight sample and at 09:30. For 24 h samples the ICC was 0.64, for overnight samples it was 0.5, and for all spot samples, it was much lower. The ICC for urinary creatinine depends on the time of day of sampling. Frozen samples from this variability biobank are open for researchers examining normal variability of their favorite biomarker(s).
Air Pollution Status in 10 Mega-Cities in China during the Initial Phase of the COVID-19 Outbreak
Authors: Ethan Crystal Jane , Mokoena Kingsley Katleho , Yu Yan
Published: March 2021

Over the past decades, urbanization and industrialization have led to a change in air quality, bringing researchers to a full realization of the damaging effects of human activities on the environment. This study focused on describing air quality during the initial phase of the Novel Coronavirus disease (COVID-19) pandemic (since there were fewer anthropogenic activities) in 10 Chinese mega-cities. Using the independent t-test, the means of air quality index (AQI) scores and individual air pollutants concentration during the outbreak were compared with the means before the outbreak. Cohen’s d was estimated to quantify how much difference was observed. Based on the AQI score, the air quality in these 10 cities ranged from excellent (Shenzhen) to light pollution (Xi’an) with 44.8 μg m−3 and 119.7 μg m−3, respectively. In comparison to the 2019 air quality, Guangzhou and Wuhan noted major differences in air quality during the outbreak. Indicators of traffic pollution, particularly NO2, were significantly lower during the outbreak in all cities. Particulate matter pollution varied, with some cities observing lower concentrations and other higher concentrations during the outbreak. There was a significant decrease in air pollution levels during the outbreak. More researchers should observe changes in air quality during peculiar or major events. Implementation of stringent regulation on vehicle use should be considered in mega-cities. Relevant findings should be employed in emphasizing the detrimental effects of anthropogenic activities and support the need for stringent emission control regulations.
Retrospective Study: Lateral Ridge Augmentation Using Autogenous Dentin: Tooth-Shell Technique vs. Bone-Shell Technique
Authors: Korsch Michael , Peichl Marco
Published: March 2021

In the literature, autogenous dentin is considered a possible alternative to bone substitute materials and autologous bone for certain indications. The aim of this proof-of-concept study was to use autogenous dentin for lateral ridge augmentation. In the present retrospective study, autogenous dentin slices were obtained from teeth and used for the reconstruction of lateral ridge defects (tooth-shell technique (TST): 28 patients (15 females, 13 males) with 34 regions and 38 implants). The bone-shell technique (BST) according to Khoury (31 patients (16 females, 15 males) with 32 regions and 41 implants) on autogenous bone served as the control. Implants were placed simultaneously in both cases. Follow-up was made 3 months after implantation. Target parameters during this period were clinical complications, horizontal hard tissue loss, osseointegration, and integrity of the buccal lamella. The prosthetic restoration with a fixed denture was carried out after 5 months. The total observation period was 5 months. A total of seven complications occurred. Of these, three implants were affected by wound dehiscences (TST: 1, BST: 2) and four by inflammations (TST: 0, BST: 4). There were no significant differences between the two groups in terms of the total number of complications. One implant with TST exhibited a horizontal hard tissue loss of 1 mm and one with BST of 0.5 mm. Other implants were not affected by hard tissue loss. There were no significant differences between the two groups. Integrity of the buccal lamella was preserved in all implants. All implants were completely osseointegrated in TST and BST. All implants could be prosthetically restored with a fixed denture 5 months after augmentation. TST showed results comparable to those of the BST. Dentin can therefore serve as an alternative material to avoid bone harvesting procedures and thus reduce postoperative discomfort of patients.
COVID-19 Epidemic: Early Shift in the Socioeconomic Profile of the Affected Population
Authors: Khlat Myriam , Le Coeur Sophie
Published: March 2021

The COVID-19 pandemic has given rise to a wealth of literature in the public health field [...]
Health Services Use and Health Outcomes among Informal Economy Workers Compared with Formal Economy Workers: A Systematic Review and Meta-Analysis
Authors: Naicker Nisha , Pega Frank , Rees David , Kgalamono Spo , Singh Tanusha
Published: March 2021

Background: There are approximately two billion workers in the informal economy globally. Compared to workers in the formal economy, these workers are often marginalised with minimal or no benefits from occupational health and safety regulations, labour laws, social protection and/or health care. Thus, informal economy workers may have higher occupational health risks compared to their formal counterparts. Our objective was to systematically review and meta-analyse evidence on relative differences (or inequalities) in health services use and health outcomes among informal economy workers, compared with formal economy workers. Methods: We searched PubMed and EMBASE in March 2020 for studies published in 1999–2020. The eligible population was informal economy workers. The comparator was formal economy workers. The eligible outcomes were general and occupational health services use, fatal and non-fatal occupational injuries, HIV, tuberculosis, musculoskeletal disorders, depression, noise-induced hearing loss and respiratory infections. Two authors independently screened records, extracted data, assessed risk of bias with RoB-SPEO, and assessed quality of evidence with GRADE. Inverse variance meta-analyses were conducted with random effects. Results: Twelve studies with 1,637,297 participants from seven countries in four WHO regions (Africa, Americas, Eastern Mediterranean and Western Pacific) were included. Compared with formal economy workers, informal economy workers were found to be less likely to use any health services (odds ratio 0.89, 95% confidence interval 0.85–0.94, four studies, 195,667 participants, I2 89%, low quality of evidence) and more likely to have depression (odds ratio 5.02, 95% confidence interval 2.72–9.27, three studies, 26,260 participants, I2 87%, low quality of evidence). We are very uncertain about the other outcomes (very-low quality of evidence). Conclusion: Informal economy workers may be less likely than formal economy workers to use any health services and more likely to have depression. The evidence is uncertain for relative differences in the other eligible outcomes. Further research is warranted to strengthen the current body of evidence and needed to improve population health and reduce health inequalities among workers.
Suicide among Polish Adolescents—A 20 Year Analysis
Authors: Orlewska Katarzyna , Orlewski Pawel , Klusek Justyna
Published: March 2021

Background: The study aimed to analyze the burden and territorial diversification of adolescent suicide and the link between suicide attempts and selected socioeconomic variables in Poland. Methods: Rates of suicide by voivodeships for years 1999–2019 were obtained from the General Police Headquarters of Poland database. The burden of premature death was expressed in years of life lost (YLL) and costs of lost productivity, which were estimated using the human capital approach. The link between suicide rates and socioeconomic determinants has been analyzed with Pearson’s correlation coefficient. Results: Over the analyzed period, an increase in suicide attempt rates and a decrease in suicide death rates have been observed. Mean YLL and costs of lost productivity per year amounted to 11,982 and 153,172,415 PLN, respectively. Territorial diversification in suicide attempt rates corresponds to the living condition, poverty, association-based capital, and satisfaction with family situation in individual voivodeships. The number of deaths due to suicide is negatively correlated with an indicator of good living conditions. Conclusions: Our findings provide quantitative evidence of the national impact of suicide and suggest that addressing social capital and poverty may have a role in preventing adolescent mortality due to suicide.
Deep Neck Infection Risk in Patients with Sleep Apnea: Real-World Evidence
Authors: Ding Meng-Chang , Hsu Cheng-Ming , Liu Stanley Yung-Chuan , Lee Yi-Chan , Yang Yao-Hsu , Liu Chia-Yen , Chang Geng-He , Tsai Yao-Te , Lee Li-Ang , Yang Pei-Rung , Li Hsueh-Yu , Tsai Ming-Shao
Published: March 2021

(1) Background: Sleep apnea may be a risk factor for deep neck infection (DNI). The objective of this study was to investigate the effects of sleep apnea on DNI. (2) Methods: In this first nationwide retrospective cohort study on the sleep apnea–DNI correlation, we obtained data from the Longitudinal Health Insurance Database 2005, a subset of the Taiwan National Health Insurance Research Database. Patients who were newly diagnosed with sleep apnea between 1997 and 2012 were identified, and patients without sleep apnea were matched at a 1:4 ratio in age, sex, socioeconomic status, and urbanization level. The primary outcome of this study was DNI occurrence. The treatment modalities for sleep apnea and the comorbidities that occurred during the study period were also analyzed. (3) Results: Our sleep apnea and comparison (non-sleep apnea) cohorts comprised 6114 and 24,456 patients, respectively. We compared the cumulative incidence of DNI between these cohorts and found a greater incidence of DNI in the sleep apnea cohort (p < 0.001). A strong sleep apnea–DNI association was found following analysis via the adjusted Cox proportional-hazards model (full model hazard ratio, 1.71; 95% confidence interval, 1.28–2.28; p < 0.001). In the subgroup analysis, sleep apnea increased DNI risk in men, in those aged < 50 years, and in those without diabetes mellitus, end-stage renal disease, liver cirrhosis, autoimmune disease, obesity, tonsillectomy, or adenotonsillectomy. (4) Conclusions: Our results confirmed sleep apnea to be an independent risk factor for DNI. Physicians should be aware of the potential occurrence of DNI in patients with sleep apnea.
Feasibility Assessment of the Let’s Walk Programme (CAMINEM): Exercise Training and Health Promotion in Primary Health-Care Settings
Authors: Mas-Alòs Sebastià , Planas-Anzano Antoni , Peirau-Terés Xavier , Real-Gatius Jordi , Galindo-Ortego Gisela
Published: March 2021

Exercise is related to many individual health outcomes but impact evaluations of exercise programmes are seldom conducted. The purpose of the study is to evaluate the feasibility of an exercise prescription intervention in primary health-care settings (CAMINEM Programme) located in two socially disadvantaged neighbourhoods. The CAMINEM was a pragmatic-driven intervention with opportunistic recruitment. It followed the 5As framework for health promotion and also the exercise training principles. Feasibility was evaluated using the RE-AIM framework (Reach, Effectiveness, Adoption, Implementation, and Maintenance). Patients with non-communicable chronic diseases participated in a 12-month home-based moderate-intensity exercise program, counselled by exercise physiologists. Participants were grouped according to their physical activity behaviour at baseline and 6-month adherence. CAMINEM reached 1.49% (n = 229) of the eligible population (N = 15374) and included a final sample of 178. Health outcomes for adhered participants followed positive patterns. Non-adhered participants visited their practitioner more compared to adhered participants. Thirty-three practitioners (40%) referred patients. Nurses referred four times more than physicians (81% and 19% respectively). The delivery of exercise prescriptions proved to be easy to complete and record by participants as well as easy to monitor and adjust by the exercise physiologists. One out of four participants adhered during the 12-month intervention. This intervention has been feasible in primary care in Catalonia, Spain, to safely prescribe home-based exercise for several conditions.
Impacts of the New Worldwide Light-Duty Test Procedure on Technology Effectiveness and China’s Passenger Vehicle Fuel Consumption Regulations
Authors: Chen Kangda , Zhao Fuquan , Liu Xinglong , Hao Han , Liu Zongwei
Published: March 2021

As a main measure to promote the development of China’s energy–saving and new energy vehicles, the Phase V fuel consumption regulation is dramatically different from the past four phases, especially in the test procedure, moving from the New European Driving Cycle (NEDC) to the worldwide harmonized light duty test cycle (WLTC) and corresponding test procedure (WLTP). The switch of test procedure will not only affect the effectiveness of technologies but also change the fuel consumption target of the industry. However, few studies have systematically investigated the impacts of the new WLTP on the Chinese market. This study establishes a “technology–vehicle–fleet” bottom–up framework to estimate the impacts of test procedure switching on technology effectiveness and regulation stringency. The results show that due to the WLTP being closer to the real driving condition and more stringent, almost all baseline vehicles in the WLTP have higher fuel consumption than that in the NEDC, and diesel vehicles are slightly more impacted than gasoline vehicles. In addition, the impacts are increased with the strengthening of electrification, where the fuel consumption of plug–in hybrid electric vehicles (PHEVs) and range-extended electric vehicles (REEVs) in the WLTP are about 6% higher than that in the NEDC. Engine technologies that gain higher effects in low load conditions, such as turbocharging and downsizing, fuel stratified injection (FSI), lean–burn, and variable valve timing (VVT), are faced with deterioration in the WLTP. Among these, the effect of turbocharging and downsizing shows a maximum decline of 8.5%. The variable compression ratio (VCR) and stoichiometric gasoline direct injection (SGDI) are among the few technologies that benefited from procedure switching, with an average improvement of 1.6% and 0.2% respectively. Except for multi–speed transmissions, which have improvement effects in the WLTP, all automatic transmissions are faced with decreases. From the perspective of the whole fleet and national regulation target, the average fuel consumption in the WLTP will increase by about 7.5% in 2025 compared to 4 L/100 km in the NEDC. According to the current planning of the Chinese government, the fuel consumption target of Phase V is set at 4.6 L/100 km in 2025, which is equivalent to loosening the stringency by 0.3 L/100 km. In Phase VI, the target of 3.2 L/100 km is maintained, which is 30.4% stricter than that of Phase V, and the annual compound tightening rate reaches 7.5%. This means that automakers need to launch their product planning as soon as possible and expand the technology bandwidth to comply with the Phase VI fuel consumption regulation, and the government should evaluate the technical feasibility before determining the evaluation methods and targets of the next phase.
Built Environment Design and People with Autism Spectrum Disorder (ASD): A Scoping Review
Authors: Tola Giulia , Talu Valentina , Congiu Tanja , Bain Paul , Lindert Jutta
Published: March 2021

Built environment design can be considered as an influential factor in the quality of life of people with autism spectrum disorder (ASD). This scoping review provides an overview of the current available literature on the relationship between people with ASD and built environment in the specific field of the design of autism-friendly spaces. The literature review allowed the identification of three main factors to be considered when designing for people with ASD—the sensory quality, the intelligibility, and the predictability of the built environment—and, for each of them, a description of the spatial requirements that have been recognized as fundamental according to the specific spatial needs of people with ASD.
Impact of Multifaceted Workplace Bullying on the Relationships between Technology Usage, Organisational Climate and Employee Physical and Emotional Health
Authors: Iftikhar Mehwish , Qureshi Muhammad Imran , Qayyum Shazia , Fatima Iram , Sriyanto Sriyanto , Indrianti Yasinta , Khan Aqeel , Dana Leo-Paul
Published: March 2021

This research article investigates the effect of organisational climate and technology usage on employees’ physiological and emotional health damage resulting from face-to-face bullying and cyberbullying at the workplace. Furthermore, we investigated emotional intelligence as a coping strategy to moderate employee physiological and emotional health damage. The research used a quantitative research design. A five-point Likert-scale questionnaire was used to collect data from a multistage sample of 500 officials from Pakistan’s four service sectors. Results revealed that organisational climate and technology usage are negatively related to face-to-face bullying and cyberbullying at the workplace. At the same time, workplace bullying adversely affects an employee’s emotional and physiological health. However, emotional intelligence can reduce an employee’s emotional health damage due to workplace bullying. Thus, we suggest incorporating emotional intelligence training at the workplace to minimise the devastating effects of face-to-face bullying and cyberbullying on employees’ physical and emotional health.
Contesting Sexual Prejudice to Support Sexual Minorities: Views of Chinese Social Workers
Authors: Kwok Diana K.
Published: March 2021

Professional development has been recognized as one of the strategies to effectively combat sexual prejudice and negative attitudes against lesbian, gay, bisexual, questioning/queer (LGBQ+) individuals and sexual minorities. Nevertheless, studies related to LGBQ+-inclusive training are rarely found in the Chinese Hong Kong context, where sexual prejudice still prevails without the establishment of antidiscrimination law. Sociocultural considerations, such as religious and parental influences, are obstacles to discussing the reduction of sexual prejudices, both within wider society and social work organizations, without institutional support. This paper aims to understand social workers’ perspectives on prejudice reduction training themes and perceived cultural barriers through qualitative in-depth interviews with 67 social workers. Qualitative thematic analysis yielded the following themes: (1) understanding sexuality; (2) initiating training legitimately; (3) contesting religious and cultural assumptions; (4) resolving value and ethical dilemma; (5) selecting relevant knowledge; (6) implementing diverse training strategies. The study suggests that social workers and service providers need to understand how sexual prejudice is manifested in Hong Kong through unique cultural forces. LGBQ+-inclusive content, addressing updated concepts and prejudice-free language, should be incorporated into the training curriculum. Intergroup contact, professional reflection, and experiential learning are suggested as training strategies (190).
Epigenetic Control of Infant B Cell Precursor Acute Lymphoblastic Leukemia
Authors: de Barrios Oriol , Parra Maribel
Published: March 2021

B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is a highly aggressive malignancy, with poorer prognosis in infants than in adults. A genetic signature has been associated with this outcome but, remarkably, leukemogenesis is commonly triggered by genetic alterations of embryonic origin that involve the deregulation of chromatin remodelers. This review considers in depth how the alteration of epigenetic profiles (at DNA and histone levels) induces an aberrant phenotype in B lymphocyte progenitors by modulating the oncogenic drivers and tumor suppressors involved in key cancer hallmarks. DNA methylation patterns have been widely studied in BCP-ALL and their correlation with survival has been established. However, the effect of methylation on histone residues can be very different. For instance, methyltransferase KMT2A gene participates in chromosomal rearrangements with several partners, imposing an altered pattern of methylated H3K4 and H3K79 residues, enhancing oncogene promoter activation, and conferring a worse outcome on affected infants. In parallel, acetylation processes provide an additional layer of epigenetic regulation and can alter the chromatin conformation, enabling the binding of regulatory factors. Therefore, an integrated knowledge of all epigenetic disorders is essential to understand the molecular basis of BCP-ALL and to identify novel entry points that can be exploited to improve therapeutic options and disease prognosis.
Cushing’s Syndrome Effects on the Thyroid
Authors: Paragliola Rosa Maria , Corsello Andrea , Papi Giampaolo , Pontecorvi Alfredo , Corsello Salvatore Maria
Published: March 2021

The most known effects of endogenous Cushing’s syndrome are the phenotypic changes and metabolic consequences. However, hypercortisolism can exert important effects on other endocrine axes. The hypothalamus–pituitary–thyroid axis activity can be impaired by the inappropriate cortisol secretion, which determinates the clinical and biochemical features of the “central hypothyroidism”. These findings have been confirmed by several clinical studies, which also showed that the cure of hypercortisolism can determine the recovery of normal hypothalamus–pituitary–thyroid axis activity. During active Cushing’s syndrome, the “immunological tolerance” guaranteed by the hypercortisolism can mask, in predisposed patients, the development of autoimmune thyroid diseases, which increases in prevalence after the resolution of hypercortisolism. However, the immunological mechanism is not the only factor that contributes to this phenomenon, which probably includes also deiodinase-impaired activity. Cushing’s syndrome can also have an indirect impact on thyroid function, considering that some drugs used for the medical control of hypercortisolism are associated with alterations in the thyroid function test. These considerations suggest the utility to check the thyroid function in Cushing’s syndrome patients, both during the active disease and after its remission.
Transcriptome Analysis Unravels Key Factors Involved in Response to Potassium Deficiency and Feedback Regulation of K+ Uptake in Cotton Roots
Authors: Yang Doudou , Li Fangjun , Yi Fei , Eneji A. Egrinya , Tian Xiaoli , Li Zhaohu
Published: March 2021

To properly understand cotton responses to potassium (K+) deficiency and how its shoot feedback regulates K+ uptake and root growth, we analyzed the changes in root transcriptome induced by low K+ (0.03 mM K+, lasting three days) in self-grafts of a K+ inefficient cotton variety (CCRI41/CCRI41, scion/rootstock) and its reciprocal grafts with a K+ efficient variety (SCRC22/CCRI41). Compared with CCRI41/CCRI41, the SCRC22 scion enhanced the K+ uptake and root growth of CCRI41 rootstock. A total of 1968 and 2539 differently expressed genes (DEGs) were identified in the roots of CCRI41/CCRI41 and SCRC22/CCRI41 in response to K+ deficiency, respectively. The overlapped and similarly (both up- or both down-) regulated DEGs in the two grafts were considered the basic response to K+ deficiency in cotton roots, whereas the DEGs only found in SCRC22/CCRI41 (1954) and those oppositely (one up- and the other down-) regulated in the two grafts might be the key factors involved in the feedback regulation of K+ uptake and root growth. The expression level of four putative K+ transporter genes (three GhHAK5s and one GhKUP3) increased in both grafts under low K+, which could enable plants to cope with K+ deficiency. In addition, two ethylene response factors (ERFs), GhERF15 and GhESE3, both down-regulated in the roots of CCRI41/CCRI41 and SCRC22/CCRI41, may negatively regulate K+ uptake in cotton roots due to higher net K+ uptake rate in their virus-induced gene silencing (VIGS) plants. In terms of feedback regulation of K+ uptake and root growth, several up-regulated DEGs related to Ca2+ binding and CIPK (CBL-interacting protein kinases), one up-regulated GhKUP3 and several up-regulated GhNRT2.1s probably play important roles. In conclusion, these results provide a deeper insight into the molecular mechanisms involved in basic response to low K+ stress in cotton roots and feedback regulation of K+ uptake, and present several low K+ tolerance-associated genes that need to be further identified and characterized.
Dietary and Protective Factors to Halt or Mitigate Progression of Autoimmunity, COVID-19 and Its Associated Metabolic Diseases
Authors: Ricordi Camillo , Pacifici Francesca , Lanzoni Giacomo , Palamara Anna Teresa , Garaci Enrico , Della-Morte David
Published: March 2021

COVID-19 is without any doubt the worst pandemic we have faced since the H1N1 virus outbreak. Even if vaccination against SARS-CoV-2 infection is becoming increasingly available, a more feasible approach for COVID-19 prevention and therapy is still needed. Evidence of a pathological link between metabolic diseases and severe forms of COVID-19 has stimulated critical reflection and new considerations. In particular, an abnormal immune response observed in certain patients with SARS-CoV-2 infection suggested possible common predisposing risk factors with autoimmune diseases such as Type 1 Diabetes (T1D). Correct supplementation with dietary factors may be key to preventing and counteracting both the underlying metabolic impairment and the complications of COVID-19. A set of agents may inhibit the cytokine storm and hypercoagulability that characterize severe COVID-19 infection: vitamin D3, omega-3 polyunsaturated fatty acids, polyphenols like pterostilbene, polydatin and honokiol, which can activate anti-inflammatory and antioxidant sirtuins pathways, quercetin, vitamin C, zinc, melatonin, lactoferrin and glutathione. These agents could be highly beneficial for subjects who have altered immune responses. In this review, we discuss the antiviral and metabolic effects of these dietary factors and propose their combination for potential applications in the prevention and treatment of COVID-19. Rigorous studies will be fundamental for validating preventive and therapeutic protocols that could be of assistance to mitigate disease progression following SARS-CoV-2 infection.
Decoding the Roles of Long Noncoding RNAs in Hepatocellular Carcinoma
Authors: Wong Lok-Sze , Wong Chun-Ming
Published: March 2021

Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies worldwide. HCC is associated with several etiological factors, including HBV/HCV infections, cirrhosis, and fatty liver diseases. However, the molecular mechanism underlying HCC development remains largely elusive. The advent of high-throughput sequencing has unveiled an unprecedented discovery of a plethora of long noncoding RNAs (lncRNAs). Despite the lack of coding capacity, lncRNAs have key roles in gene regulation through interacting with various biomolecules. It is increasingly evident that the dysregulation of lncRNAs is inextricably linked to HCC cancer phenotypes, suggesting that lncRNAs are potential prognostic markers and therapeutic targets. In light of the emerging research in the study of the regulatory roles of lncRNAs in HCC, we discuss the association of lncRNAs with HCC. We link the biological processes influenced by lncRNAs to cancer hallmarks in HCC and describe the associated functional mechanisms. This review sheds light on future research directions, including the potential therapeutic applications of lncRNAs.
The Role of Phospholipase C in GABAergic Inhibition and Its Relevance to Epilepsy
Authors: Kim Hye Yun , Suh Pann-Ghill , Kim Jae-Ick
Published: March 2021

Epilepsy is characterized by recurrent seizures due to abnormal hyperexcitation of neurons. Recent studies have suggested that the imbalance of excitation and inhibition (E/I) in the central nervous system is closely implicated in the etiology of epilepsy. In the brain, GABA is a major inhibitory neurotransmitter and plays a pivotal role in maintaining E/I balance. As such, altered GABAergic inhibition can lead to severe E/I imbalance, consequently resulting in excessive and hypersynchronous neuronal activity as in epilepsy. Phospholipase C (PLC) is a key enzyme in the intracellular signaling pathway and regulates various neuronal functions including neuronal development, synaptic transmission, and plasticity in the brain. Accumulating evidence suggests that neuronal PLC is critically involved in multiple aspects of GABAergic functions. Therefore, a better understanding of mechanisms by which neuronal PLC regulates GABAergic inhibition is necessary for revealing an unrecognized linkage between PLC and epilepsy and developing more effective treatments for epilepsy. Here we review the function of PLC in GABAergic inhibition in the brain and discuss a pathophysiological relationship between PLC and epilepsy.