bluuebunny/crossref_metadata_2025 huggingface dataset:
Performance of Automated Oral Cancer Screening Algorithm in Tobacco Users vs. Non-Tobacco Users
Authors: Yang Susan Meishan , Song Bofan , Wink Cherie , Abouakl Mary , Takesh Thair , Hurlbutt Michelle , Dinica Dana , Davis Amber , Liang Rongguang , Wilder-Smith Petra
Published: March 2023

Oral non-neoplastic and neoplastic lesions have similar clinical manifestations, increasing the risk of inaccurate screening decisions that adversely affect oral cancer (OC) outcomes. Tobacco-use-related changes in the oral soft tissues may affect the accuracy of “smart” oral screening modalities. Because smoking is such a strong predictor of OC risk, it may overwhelm the impact of other variables on algorithm performance. The objective was to evaluate the screening accuracy in tobacco users vs. non-users of a previously developed prototype smartphone and machine-learning algorithm-based oral health screening modality. 318 subjects with healthy mucosa or oral lesions were allocated into either a “tobacco smoker” group or a “tobacco non-smoker” group. Next, intraoral autofluorescence (AFI) and polarized white light images (pWLI), risk factors as well as clinical signs and symptoms were recorded using the prototype screening platform. OC risk status as determined by the algorithm was compared with OC risk evaluation by an oral medicine specialist (gold standard). The screening platform achieved 80.0% sensitivity, 87.5% specificity, 83.67% agreement with specialist screening outcome in tobacco smokers, and 62.1% sensitivity, 82.9% specificity, 73.1% agreement with specialist screening outcome in non-smokers. Tobacco use should be carefully weighted as a variable in the architecture of any imaging-based screening algorithm for OC risk.
Effect of Protective Coatings on Wooden Elements Exposed to a Small Ignition Initiator
Authors: Gašpercová Stanislava , Marková Iveta , Vandlíčková Miroslava , Osvaldová Linda Makovická , Svetlík Jozef
Published: March 2023

This paper presents the results of monitoring the behaviour of selected wood species exposed to a small ignition initiator. We specifically aimed to investigate the effect of retardant coating on the combustion process of the wood species spruce (Picea abies), red spruce (Larix decidua), Scots pine (Pinus sylvestris), ash (Fraxinus excelsior L.), beech (Fagus sylvatica), and oak (Quercus robur) when exposed to a small initiator of ignition. At the same time, we studied the effect of different types of fungicidal agents (based on N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine; alkyl (C12–16) dimethylbenzyl ammonium chloride or 3-iodo-prop-2-inylbutylcarbamate) on the effectiveness of a flame retardant (containing ferric phosphate) applied to the selected fungicide-treated wood samples. The experimentally obtained mass-loss and flame-spread results were statistically evaluated using the QtiPlot software program. A significant dependence of mass loss and surface flame spread on wood species was the primary focus. It was only confirmed for the surface-flame-spread variable. The dependence of the effect of fungicide treatment on the effectiveness of the selected retardant was confirmed. Fungicidal coatings with the active ingredient alkyl/benzyl (C12–16) dimethylbenzyl ammonium chloride indicated the lowest mass loss rate and flame spread for all wood samples.
Shock and Spallation Behavior of a Compositionally Complex High-Strength Low-Alloy Steel under Different Impact Stresses
Authors: Lu Ye , Li Junbao
Published: March 2023

The shock and spalling behavior of a compositionally complex high strength low-alloy steel (HSLA) was studied using plate impact testing. The free surface velocity of the specimen in the range of 194~938 m/s was measured by a displacement interferometer system for any reflector (DISAR). The Hugoniot elastic limit (HEL), spallation fracture and microstructural evolution of the HSLA under an impact stress of 3.04~18.66 GPa were analyzed. Shock Hugoniots were obtained from the measured particle velocities and calculated shock velocities. The velocity curves show clear signs of HEL and velocity fallback, indicating a transition from elastic to plastic and spalling behavior. When the impact velocity exceeds 757 m/s, the particle velocity rises to the peak and then increases again, indicating that an α→ε phase transition occurred, with a threshold of 13.51 GPa. It was found that the impact velocity is linearly related to the particle velocity of the HSLA. As the impact stress increased, the HEL remained within the range of 1.32~1.50 GPa, while the spalling strength presented an upward trend with the increasing impact stresses. Metallographic analysis shows that the impact failure is dominated by brittle fracture at lower velocities, while more ductile fracture occurs at higher velocities.
Recent Applications of Smartphone and Smart Glasses in Applied Science and Engineering
Authors: Choi Yosoon
Published: March 2023

Today, more than 3 [...]
Learning Activities with Plants and Technology: A Systematic Literature Review
Authors: Leo-Ramírez Alejandro , Alvarez Jose , Pérez Marina , Greller Wolfgang , Tabuenca Bernardo
Published: March 2023

The increase in remote work and study modalities in recent years has changed our indoor physical spaces. Key variables such as air quality, temperature, or well-being in general have acquired special relevance when designing workspaces. In this context, plants can play an active role in moderating these variables and providing well-being to the people who live in these spaces. UNESCO, when framing its 2030 agenda, highlighted the importance of promoting environmental awareness at the educational level (Sustainable Development Goals 3, 4, and 11). The scientific literature shows that the potential of plants is not sufficiently well addressed in educational contexts. Therefore, this review explores activities in which plants are used as a deliberate object of attention in learning contexts. The results show what learning activities have been carried out, what kind of plants have been used in the activities, and what technologies have supported those activities. The results provide a clear vision of the potential of plants to naturalize indoor learning spaces and to promote environmental awareness. This work aims to provide cues for further research on green education towards a sustainable society.
The Role of Fluid Overpressure on the Fracture Slip Mechanism Based on Laboratory Tests That Stimulating Reservoir-Induced Seismicity
Authors: Zhu Yujie , Xu Chen , Song Danqing , Liu Xiaoli , Wang Enzhi
Published: March 2023

A critically stressed fracture will slide in response to the increase in fluid pressure inside the fracture while impounding, which will trigger induced seismicity. The mechanism of fluid overpressure is regarded as a significant factor in the reaction of the fracture slip after water diffusing. This study uses a shearing test with a cylinder of granite, with 100 mm height and 50 mm diameter, under the condition of hydraulic-mechanic (HM) coupling to figure out how fluid overpressure alters the mechanical behavior of the critically stressed fracture. The cyclic water pressurization simulates periodical impounding in the water reservoir. Results show that several slip events happen when water pressure continues to rise higher than the stable state. The change of roughness also indicates the deterioration of the fracture surface while sliding. According to the results, we conclude that the difference between inlet pressure and outlet pressure leads to an overpressure of the fracture, promoting a series of slips and induced seismicity. Hydraulic energy is introduced to explain the relationship between the input and output energy, which is also strong evidence to illustrate that fluid overpressure is a crucial mechanism in reservoir-induced seismicity.
Case Study and Risk Assessment of Water Inrush Disaster in Qingdao Metro Line 4
Authors: Zhang Yongjun , Zhang Weiguo , Xia Huangshuai , Gong Bin , Liu Fei , Zhang Jiahui , Liu Kai
Published: March 2023

Water inrush is one of the most frequent and catastrophic hazards in tunnel engineering, and poses serious threats to the safety of engineering and personnel. This paper presents a case study of a water inrush and ground collapse in the Qingdao Metro Line 4, which caused a cave-in with the diameter and depth of about 30 m and 6 m, respectively. Based on the field data and numerical modelling, the causes of the disaster were analyzed. A numerical model was used to analyze the changes of surface settlement, vault settlement and water pressure during the tunnel excavation. The results of the study indicate that the cause of this disaster was the failure of the tunnel vault surrounding rock caused by the weakening of the tunnel surrounding rock and water pressure, which in turn triggered the water inrush in the tunnel and caused a large volume of surface collapse. As the tunnel was excavated from the slightly weathered area to the strongly weathered area, the vault settlement increased, and the influence zone expanded towards the surface due to the continuous decrease in the strength of the surrounding rock. In particular, a negative pore water pressure zone was formed in a certain area around the tunnel during the water inrush. The negative pressure zone caused the surrounding groundwater to converge here, leading to an increase in the amount of water inflow, which also increased the scope and scale of the impact of this disaster. A risk assessment method for water inrush in tunnels is proposed. According to the geological and engineering characteristics of Qingdao area, the evaluation index system of tunnel water inrush risk was established. An RBF neural network was improved by gray correlation analysis and a PAM clustering algorithm to establish the tunnel water inrush risk assessment model. Comparing the evaluation data with the actual data, the prediction data of a traditional RBF neural network and a BP neural network, the accuracy and reliability of the model were verified. This study has value in reducing the occurrence of water inrush in a composite formation tunnel.
Assessment of Land Surface Schemes from the WRF-Chem for Atmospheric Modeling in the Andean Region of Ecuador
Authors: Parra Rene
Published: March 2023

Surface interactions occur near the land–atmosphere interface, thus affecting the temperature, convection, boundary layer, and stability of the atmosphere. A proper representation of surface interactions is a crucial component for numerical atmospheric and air quality modeling. We assessed four land surface schemes—1. 5–layer thermal diffusion scheme (1 5-Layer); 2. unified Noah land surface model (2 Noah); 3. rapid update cycle (3 RUC) land surface model; and 4. Pleim–Xiu land surface model (4 Pleim–Xiu)—from the Weather Research and Forecasting with Chemistry (WRF-Chem V3.2) model for the purposes of atmospheric modeling in Cuenca, which is a region with a complex topography and land use configuration and which is located in the Southern Andean region, in Ecuador. For this purpose, we modeled the meteorological and air quality variables during September 2014. It was found that the meteorological and short-term air quality variables were better modeled through the 2 Noah scheme. Long-term (mean monthly) air quality variables were better modeled by the 1 5–Layer and 3 RUC options. On average, the 2 Noah scheme was better at modeling meteorology and air quality. In addition, we assessed the 2 Noah scheme combined with the urban canopy model (UCM) (5 Noah UCM), which was developed as an option to represent the urban effects at a subgrid-scale. Results indicated that the performance of the 5 Noah UCM scheme was not better at modeling than the 2 Noah scheme alone. Moreover, the 5 Noah UCM scheme notably decreased the modeling performance for carbon monoxide and fine particulate matter. These results complement previous assessments of other schemes, allowing us to recommend a basic configuration of parameters for atmospheric modeling in the Andean region of Ecuador.
Mid-Latitude Jet Response to Pan-Arctic and Regional Arctic Warming in Idealized GCM
Authors: Yang Gun-Hwan , Moon Woosok , Noh Hayeon , Kim Baek-Min
Published: March 2023

To study the dynamical mechanism by which Arctic amplification affects extreme weather events in mid-latitude, we investigated the local and remote circulation response to pan-Arctic and regional Arctic thermal forcing. A comprehensive atmospheric GCM (General Circulation Model) coupled to a slab mixed-layer ocean model is used for the experiment. With the increasing thermal forcing in the pan-Arctic configuration, the mid-latitude jet tends to shift equatorward, mainly due to the southward shift of the convergence zone of eddy-heat flux and eddy-momentum flux. From the regional Arctic forced experiments, zonal mean response is similar to the response from the pan-Arctic configuration. The non-zonal response is characterized by the 300 hPa circumpolar zonal wind of wavenumber-1 structure, which establishes an enhanced wavier mid-latitude jet. In the polar region at 300 hPa, regional thermal forcing drives a distinct east–west dipole circulation pattern, in which anticyclonic circulation is located to the west of the thermal forcing, and cyclonic circulation is located to the east. The lower-level circulation shows the opposite pattern to the upper-level circulation in the polar region. While the strength of circulation increases with gradual thermal forcing, the overall dipole pattern is unchanged. In regional warming simulation, compared to the pan-Arctic warming, increasing residual heat flux in a dipole pattern causes enhanced heat advection to mid-latitude.
Study of Relative Humidity Vertical Distribution Characteristics before Precipitation by Microwave Radiometer Data over Southeast China
Authors: Yu Yongjiang , Zou Yan , Pan Weihua
Published: March 2023

We investigated the relative humidity (RH) vertical distribution characteristics before precipitation using microwave radiometer measurements over southeast China in 2021. The superposed epoch method is used to analyze the profile and vertical statistical characteristics and evolution of RH during precipitation events. There is a shallow, high-humidity area on the ground, with a thickness of about 0.1–0.2 Km, from 12 to 8 h before precipitation. An obvious dry layer appears in the lower layer near the ground 8–0 h before precipitation, with a thickness of about 1 km and humidity of less than 80%, which continues until the appearance of precipitation. The water vapor content in the air begins to accumulate and the humidity increases before the occurrence of LRs, MRs, and HRs, classified by total rainfall. The SDPs, MDPs, and LDPs, which are classified by precipitation duration, showed more obvious and significant characteristics of humidity increase. The statistical analysis of the 44 precipitation cases shows that the relative humidity on the ground and in the air increases significantly before precipitation, and the vertical distribution of the relative humidity and the increase in the water vapor content in the air have a more direct and obvious impact on the precipitation duration. The deep and high-humidity area of 2–4 km is conducive to maintaining the precipitation process for a long time.
Airglow Imaging Observations of Plasma Blobs: Merging and Bifurcation during Solar Minimum over Tropical Region
Authors: Adebayo Micheal O. , Pimenta Alexandre A. , Savio Siomel , Nyassor Prosper K.
Published: March 2023

Plasma blobs are night-time ionospheric irregularities whose generation mechanism is still under investigation. A large number of observations highlighted several aspects of their morphology and dynamics. However, the plasma blobs have not been attributed convincingly to a known mechanism. We analyzed the OI 630.0 nm emission images during March and October of 2019 and 2020 (minimum solar activity) using the ground-based all-sky imager at ZF-2 (2.58° S, 60.22° W) in the Amazon region of Brazil. The novelties of the present study are the rarely reported observation of both plasma blob merging and bifurcation. We studied the evolutional dynamics of plasma blobs and observed that blobs are distinct phenomena with unique properties. We attribute the merging of plasma blobs to the “wind reversion effect” (WRE) mechanism caused by a change in the direction of the zonal thermospheric wind from east to west. In some cases, the slower-drifting plasma blobs may merge with the faster ones. Moreover, blobs were observed initially bifurcating at the topside and later divided into two. The activity of the polarized electric field inside the plasma bubble mapping along the magnetic field lines is possibly responsible for the blob’s bifurcation. Subjecting the two features of ionospheric plasma blobs to simulation may reveal further the physics of blobs’ merging and bifurcation.
Social Representations of “Tinnitus” and “Health” among Individuals with Tinnitus Seeking Online Psychological Interventions
Authors: Manchaiah Vinaya , Chundu Srikanth , Ratinaud Pierre , Andersson Gerhard , Beukes Eldre W.
Published: March 2023

(1) Objective: Social representations theory (SRT) is a body of theory within social psychology concerned with how individuals, groups, and communities collectively make sense of socially relevant or problematic issues, ideas, and practices. SRT has been increasingly sued in the area of health and disability. The current study examined the social representations of “tinnitus” and “health” among individuals with tinnitus who are seeking online psychological interventions. (2) Materials/Method: The data were gathered using a free association task about their “tinnitus” and “health” from 399 individuals with tinnitus. The data were analyzed using both qualitative and quantitative analyses methods. (3) Results: The responses resulted in 39 and 30 categories respectively, for “tinnitus” and “health”. The most commonly occurring categories for tinnitus included: descriptions of tinnitus (18%), annoying (13.5%), persistent (8%), and distracting (5%). The most commonly occurring categories for health included: content (12%), conditions (8%), active (7%), take control (6%), and overweight (5%). The responses to tinnitus had predominantly negative connotations (i.e., 76.9%) whereas a larger proportion of responses toward their health was related to positive connotations (i.e., 46.4%). These frequently occurring items were also dominant in similarities analysis. Prototypical analysis of tinnitus responses identified categories horrible and bothersome to be key items in the central zone. The categories in central zone of health responses included: content, active, healthy, grateful, and overweight. (4) Conclusions: Individuals with tinnitus have very negative view of their tinnitus impacting their psychological status. Tinnitus management should focus on reducing the negative associations toward their tinnitus and strengthen the positive aspects related to their general health.
On Several Parameters of Super Line Graph L2(G)
Authors: Meng Jiawei , Wu Baoyindureng , Ma Hongliang
Published: March 2023

The super line graph of index r, denoted by Lr(G), is defined for any graph G with at least r edges. Its vertices are the sets of r edges of G, and two such sets are adjacent if an edge of one is adjacent to an edge of the other. In this paper, we give an explicit characterization for all graphs G with L2(G) being a complete graph. We present lower bounds for the clique number and chromatic number of L2(G) for several classes of graphs. In addition, bounds for the domination number of L2(G) are established in terms of the domination number of the line graph L(G) of a graph. A number of related problems on L2(G) are proposed for a further study.
New Arithmetic Operations of Non-Normal Fuzzy Sets Using Compatibility
Authors: Wu Hsien-Chung
Published: March 2023

The new arithmetic operations of non-normal fuzzy sets are studied in this paper by using the extension principle and considering the general aggregation function. Usually, the aggregation functions are taken to be the minimum function or t-norms. In this paper, we considered a general aggregation function to set up the arithmetic operations of non-normal fuzzy sets. In applications, the arithmetic operations of fuzzy sets are always transferred to the arithmetic operations of their corresponding α-level sets. When the aggregation function is taken to be the minimum function, this transformation is clearly realized. Since the general aggregation function was adopted in this paper, the concept of compatibility with α-level sets is needed and is proposed, which can cover the conventional case using minimum functions as the special case.
Modelling and Analysis of Hybrid Transformation for Lossless Big Medical Image Compression
Authors: Xue Xingsi , Marappan Raja , Raju Sekar Kidambi , Raghavan Rangarajan , Rajan Rengasri , Khalaf Osamah Ibrahim , Abdulsahib Ghaida Muttashar
Published: March 2023

Due to rapidly developing technology and new research innovations, privacy and data preservation are paramount, especially in the healthcare industry. At the same time, the storage of large volumes of data in medical records should be minimized. Recently, several types of research on lossless medically significant data compression and various steganography methods have been conducted. This research develops a hybrid approach with advanced steganography, wavelet transform (WT), and lossless compression to ensure privacy and storage. This research focuses on preserving patient data through enhanced security and optimized storage of large data images that allow a pharmacologist to store twice as much information in the same storage space in an extensive data repository. Safe storage, fast image service, and minimum computing power are the main objectives of this research. This work uses a fast and smooth knight tour (KT) algorithm to embed patient data into medical images and a discrete WT (DWT) to protect shield images. In addition, lossless packet compression is used to minimize memory footprints and maximize memory efficiency. JPEG formats’ compression ratio percentages are slightly higher than those of PNG formats. When image size increases, that is, for high-resolution images, the compression ratio lies between 7% and 7.5%, and the compression percentage lies between 30% and 37%. The proposed model increases the expected compression ratio and percentage compared to other models. The average compression ratio lies between 7.8% and 8.6%, and the expected compression ratio lies between 35% and 60%. Compared to state-of-the-art methods, this research results in greater data security without compromising image quality. Reducing images makes them easier to process and allows many images to be saved in archives.
For Heart Rate Assessments from Drone Footage in Disaster Scenarios
Authors: Mösch Lucas , Barz Isabelle , Müller Anna , Pereira Carina B. , Moormann Dieter , Czaplik Michael , Follmann Andreas
Published: March 2023

The ability to use drones to obtain important vital signs could be very valuable for emergency personnel during mass-casualty incidents. The rapid and robust remote assessment of heart rates could serve as a life-saving decision aid for first-responders. With the flight sensor data of a specialized drone, a pipeline was developed to achieve a robust, non-contact assessment of heart rates through remote photoplethysmography (rPPG). This robust assessment was achieved through adaptive face-aware exposure and comprehensive de-noising of a large number of predicted noise sources. In addition, we performed a proof-of-concept study that involved 18 stationary subjects with clean skin and 36 recordings of their vital signs, using the developed pipeline in outdoor conditions. In this study, we could achieve a single-value heart-rate assessment with an overall root-mean-squared error of 14.3 beats-per-minute, demonstrating the basic feasibility of our approach. However, further research is needed to verify the applicability of our approach in actual disaster situations, where remote photoplethysmography readings could be impacted by other factors, such as blood, dirt, and body positioning.
Interleukin-18 and Gelsolin Are Associated with Acute Kidney Disease after Cardiac Catheterization
Authors: Kuo Po-Yen , Tsai Kai-Fan , Wu Po-Jung , Hsu Pai-Chin , Wu Chien-Hsing , Lee Wen-Chin , Fang Hsiu-Yu , Fang Chih-Yuan , Chung Sheng-Ying , Chen Yung-Lung , Chiou Terry Ting-Yu
Published: March 2023

Patients undergoing cardiac catheterization are at high risk of post-procedure acute kidney injury (AKI) and may experience persistent renal damage after an initial insult, a state known as acute kidney disease (AKD). However, the association between AKD and urinary renal biomarkers has not yet been evaluated in this population. We enrolled 94 patients who underwent elective cardiac catheterization to investigate patterns of urinary renal biomarkers and their associations with post-procedure AKD. Serial urinary renal biomarker levels were measured during pre-procedure, early post-procedure (12–24 h), and late post-procedure (7–10 days) periods. In our investigation, 42.55% of the enrolled patients developed AKD during the late post-procedure period. While the liver-type free-fatty-acid-binding protein level increased sharply during the early post-procedure period, it returned to baseline during the late post-procedure period. In contrast, interleukin-18 (IL-18) levels increased steadily during the post-procedure period. Early post-procedure ratios of IL-18 and gelsolin (GSN) were independently associated with subsequent AKD (odds ratio (95% confidence interval), 4.742 (1.523–14.759) for IL-18 ratio, p = 0.007; 1.812 (1.027–3.198) for GSN ratio, p = 0.040). In conclusion, post-procedure AKD is common and associated with early changes in urinary IL-18 and GSN in patients undergoing cardiac catheterization.
Melatonin in Health and Disease: A Perspective for Livestock Production
Authors: Contreras-Correa Zully E. , Messman Riley D. , Swanson Rebecca M. , Lemley Caleb O.
Published: March 2023

Mounting evidence in the literature indicates an important role of endogenous and exogenous melatonin in driving physiological and molecular adaptations in livestock. Melatonin has been extensively studied in seasonally polyestrous animals whereby supplementation studies have been used to adjust circannual rhythms in herds of animals under abnormal photoperiodic conditions. Livestock undergo multiple metabolic and physiological adaptation processes throughout their production cycle which can result in decreased immune response leading to chronic illness, weight loss, or decreased production efficiency; however, melatonin’s antioxidant capacity and immunostimulatory properties could alleviate these effects. The cardiovascular system responds to melatonin and depending on receptor type and localization, melatonin can vasodilate or vasoconstrict several systemic arteries, thereby controlling whole animal nutrient partitioning via vascular resistance. Increased incidences of non-communicable diseases in populations exposed to circadian disruption have uncovered novel pathways of neurohormones, such as melatonin, influence health, and disease. Perturbations in immune function can negatively impact the growth and development of livestock which has been examined following melatonin supplementation. Specifically, melatonin can influence nutrient uptake, circulating nutrient profiles, and endocrine profiles controlling economically important livestock growth and development. This review focuses on the physiological, cellular, and molecular implications of melatonin on the health and disease of domesticated food animals.
Current SARS-CoV-2 Protective Strategies for Healthcare Professionals
Authors: Ting Miriam , Molinari John A. , Suzuki Jon B.
Published: March 2023

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the Coronavirus disease 2019 (COVID-19). COVID-19 was first reported in China in December 2019. SARS-CoV-2 is highly contagious and spread primarily via an airborne route. Hand hygiene, surgical masks, vaccinations and boosters, air filtration, environmental sanitization, instrument sterilization, mouth rinses, and social distancing are essential infection control measures against the transmission of SARS-CoV-2. This paper aims to provide healthcare professionals with evidence-based protective strategies.
Detection of Alpha Fetoprotein Based on AIEgen Nanosphere Labeled Aptamer Combined with Sandwich Structure of Magnetic Gold Nanocomposites
Authors: Liu Lei , Wang Huixing , Sulemana Husseini , Xie Bing , Gao Li
Published: March 2023

As a biomarker, alpha-fetoprotein (AFP) is valuable for detecting some tumors in men, non-pregnant women, and children. However, the detection sensitivity in some methods needs to be improved. Therefore, developing a simple, reliable, and sensitive detection method for AFP is important for non-malignant diseases. An aptamer binding was developed based on aggregation-induced emission luminogen (AIEgen) nanosphere labeled with Fe3O4@MPTMS@AuNPs. AFP was detected with a sandwich structure of AuNPs magnetic composite particles. An aggregation-induced emission (AIE) molecule and polystyrene (PS) nanosphere complex were assembled, enhancing the fluorescence and improving the sensitivity of detection. The limit of detection (LOD) was at a given level of 1.429 pg/mL, which can best be achieved in serum samples. Finally, the results obtained showed the complex to be promising in practical applications.
Polydopamine Nanoparticles-Based Three-Line Lateral Flow Immunoassay for COVID-19 Detection
Authors: Liu Zhe , Cao Chaoyu , Tong Haoyang , You Minli
Published: March 2023

Currently, the global trend of several hundred thousand new confirmed COVID-19 patients per day has not abated significantly. Serological antibody detection has become an important tool for the self-screening of people. While the most commonly used colorimetric lateral flow immunoassay (LFIA) methods for the detection of COVID-19 antibodies are limited by low sensitivity and a lack of quantification ability. This leads to poor accuracy in the screening of early COVID-19 patients. Therefore, it is necessary to develop an accurate and sensitive autonomous antibody detection technique that will effectively reduce the COVID-19 infection rate. Here, we developed a three-line LFIA immunoassay based on polydopamine (PDA) nanoparticles for COVID-19 IgG and IgM antibodies detection to determine the degree of infection. The PDA-based three-line LFIA has a detection limit of 1.51 and 2.34 ng/mL for IgM and IgG, respectively. This assay reveals a good linearity for both IgM and IgG antibodies detection and is also able to achieve quantitative detection by measuring the optical density of test lines. In comparison, the commercial AuNP-based LFIA showed worse quantification results than the developed PDA-based LFIA for low-concentration COVID-19 antibody samples, making it difficult to distinguish between negative and positive samples. Therefore, the developed PDA-based three-line LFIA platform has the accurate quantitative capability and high sensitivity, which could be a powerful tool for the large-scale self-screening of people.
Noninvasive Brain Stimulation for Neurorehabilitation in Post-Stroke Patients
Authors: Li Kun-Peng , Wu Jia-Jia , Zhou Zong-Lei , Xu Dong-Sheng , Zheng Mou-Xiong , Hua Xu-Yun , Xu Jian-Guang
Published: March 2023

Characterized by high morbidity, mortality, and disability, stroke usually causes symptoms of cerebral hypoxia due to a sudden blockage or rupture of brain vessels, and it seriously threatens human life and health. Rehabilitation is the essential treatment for post-stroke patients suffering from functional impairments, through which hemiparesis, aphasia, dysphagia, unilateral neglect, depression, and cognitive dysfunction can be restored to various degrees. Noninvasive brain stimulation (NIBS) is a popular neuromodulatory technology of rehabilitation focusing on the local cerebral cortex, which can improve clinical functions by regulating the excitability of corresponding neurons. Increasing evidence has been obtained from the clinical application of NIBS, especially repetitive transcranial magnetic stimulation (rTMS) and transcranial direct current stimulation (tDCS). However, without a standardized protocol, existing studies on NIBS show a wide variation in terms of stimulation site, frequency, intensity, dosage, and other parameters. Its application for neurorehabilitation in post-stroke patients is still limited. With advances in neuronavigation technologies, functional near-infrared spectroscopy, and functional MRI, specific brain regions can be precisely located for stimulation. On the basis of our further understanding on neural circuits, neuromodulation in post-stroke rehabilitation has also evolved from single-target stimulation to co-stimulation of two or more targets, even circuits and the network. The present study aims to review the findings of current research, discuss future directions of NIBS application, and finally promote the use of NIBS in post-stroke rehabilitation.
Determinants of Preschool Teachers’ Knowledge-Sharing Behavior from a Thinking Style Perspective
Authors: Cheng Lu , Wu Dang , Cao Junwei
Published: March 2023

Knowledge sharing not only promotes communication among teachers to achieve self-professional growth but also facilitates knowledge innovation. Thus, knowledge sharing among preschool teachers deserves attention. This study explored the factors influencing preschool teachers’ knowledge-sharing behaviors. A questionnaire was administered to 297 preschool teachers using a Norm Activation Model from a thinking style perspective. Data analysis was performed using partial least square-structural equation modelling (PLS-SEM). The findings indicate that executive thinking style preschool teachers showed a significant positive influence of awareness of consequences; legislative thinking style preschool teachers showed a significant positive influence of awareness of consequences and ascription of responsibility; awareness of consequences had a significant positive influence on ascription of responsibility; awareness of consequences and ascription of responsibility had a significant positive influence on personal norms; and personal norms had a significant positive influence on knowledge-sharing behavior. Meanwhile, the influence of executive thinking style on ascription of responsibility, legislative thinking style on ascription of responsibility, and awareness of consequences on personal norms emerged as significantly different among preschool teachers in two different contexts: interpersonal sharing and Internet sharing. This study confirmed the factors influencing preschool teachers’ knowledge-sharing behaviors from a thinking style perspective and provides suggestions for improving preschool teachers’ knowledge-sharing behaviors.
The Influence of Emotion Induced by Accidents and Incidents on Pilots’ Situation Awareness
Authors: Lu Tianjiao , Li Yuan , Zhou Chenchen , Tang Menghan , You Xuqun
Published: March 2023

(1) Background: This study examines the differences in emotions induced by accidents and incidents as emotional stimuli and the effects on pilot situation awareness (SA) after induction. (2) Method: Forty-five jet pilots were randomly divided into three groups after which their emotions were induced using the pictures of accident, incident, and neutral stimulus, respectively. (3) Results: The conditions of accidents and incidents both induced changes in the pilots’ happiness and sadness and the changes in the emotion were regulated by the emotional intelligence of pilots in the high SA group. The emotion induction, which caused a direct change in pilot’s happiness and fear, resulted in conditions that indirectly affected level 1 of SA in pilots. (4) Conclusions: The research elucidates the difference between accident and incident in inducing pilot emotions, and reminds us that SA level exerts the regulating effects on the same emotional induction conditions.
Comparative Analysis of Building Information Modeling (BIM) Patterns and Trends in the United Arab Emirates (UAE) with Developed Countries
Authors: Abdalla Salem Buhashima , Rashid Mamun , Yahia Moohammed Wasim , Mushtaha Emad , Opoku Alex , Sukkar Ahmad , Maksoud Aref , Hamad Rawan
Published: March 2023

This research seeks to gain a deeper understanding of the current ecosystem of BIM technology and its utilization in the UAE. Since the UAE has been an early adopter of BIM, its utilization of BIM is now likely to reach that of developed nations. By adopting the BIM case study analysis framework, two sets of projects of similar categories in terms of functionality and typology were selected and mapped. One set represents projects within the UAE, and the second represents projects from developed nations utilizing BIM during the projects’ lifecycle (LC). Incorporating expert interviews to triangulate the results collected from the case study analysis will further contribute to developing a more in-depth comprehension of BIM use in the UAE. The three significant inferences can be drawn from the findings. Firstly, in the UAE, stakeholders utilize BIM fragmentarily in different stages. Secondly, the full extent of BIM utilization over the project’s LC is not pursued. Thirdly, no effective BIM standards or protocols exist. Even though international firms are the main drivers of UAE BIM adoption and utilization, UAE BIM is still in its infancy, necessitating a more collaborative effort to realize the holistic benefits of the BIM process. The findings could be utilized to identify industry standards and guiding principles for BIM implementation in the UAE and help identify areas for improvement. It also provides valuable insights into how BIM can be used more effectively in the country and how its use can be promoted. Additionally, it could be used as a guide for owners/asset managers and AEC professionals in the UAE looking to implement BIM in their projects.
Fatigue Test and Unified Fatigue Life Calculation of Q460C Steel Notched Plates
Authors: Lv Fengjun , Wang Wanzhen , Zhao Wei
Published: March 2023

In the present study, a total of 20 fatigue tests on notched plates of Q460C steel were carried out, where the effects of relative stress amplitude, Δσ/fy, and relative nominal maximum stress, σmax/fy, on the fatigue life of these notched plates were carefully examined. Theoretical analyses and numerical simulations were subsequently conducted, based on an ellipsoidal fracture model originally proposed by the second author, which has been validated for use as a fracture criterion of fatigue crack, to investigate the fatigue cracking in the Q460C steel notched plates. The theoretical model was further developed to estimate the fatigue life of the Q460C steel notched plates using a unified crack growth approach originally proposed by the second author. Based on the theoretical and simulated results, the accuracy of the unified crack growth approach, and the allowable stress fatigue life formula recommended in China’s code GB50017-2017, were assessed. The comparisons indicate that the unified crack growth approach is able to provide a reliable fatigue life assessment for the Q460C steel notched plates.
PKCθ Regulates Pituitary Adenoma Bone Invasion by Activating Osteoclast in NF-κB/IL-1β-Dependent Manner
Authors: Wang Quanji , Lei Zhuowei , Wang Zihan , Jiang Qian , Zhang Zhuo , Liu Xiaojin , Xing Biao , Li Sihan , Guo Xiang , Liu Yanchao , Li Xingbo , Shu Kai , Zhang Huaqiu , Huang Yimin , Lei Ting
Published: March 2023

Background: Pituitary adenoma (PA) bone invasion results in adverse outcomes, such as reduced rates of complete surgical resection and biochemical remission as well as increased recurrence rates, though few studies have been conducted. Methods: We collected clinical specimens of PAs for staining and statistical analysis. Evaluation of the ability of PA cells to induce monocyte–osteoclast differentiation by coculturing PA cells with RAW264.7 in vitro. An in vivo model of bone invasion was used to simulate the process of bone erosion and evaluate the effect of different interventions in alleviating bone invasion. Results: We found an overactivation of osteoclasts in bone-invasive PAs and concomitant aggregation of inflammatory factors. Furthermore, activation of PKCθ in PAs was established as a central signaling promoting PA bone invasion through the PKCθ/NF-κB/IL-1β pathway. By inhibiting PKCθ and blocking IL1β, we were able to significantly reverse bone invasion in an in vivo study. Meanwhile, we also found that celastrol, as a natural product, can obviously reduce the secretion of IL-1β as well as alleviate the progression of bone invasion. Conclusions: By activating the PKCθ/NF-κB/IL-1β pathway, pituitary tumors are able to induce monocyte–osteoclast differentiation in a paracrine manner and promote bone invasion, which can be alleviated by celastrol.
A Comprehensive Review of Prognostic Factors in Patients with Gastric Adenocarcinoma
Authors: Mantziari Styliani , St Amour Penelope , Abboretti Francesco , Teixeira-Farinha Hugo , Gaspar Figueiredo Sergio , Gronnier Caroline , Schizas Dimitrios , Demartines Nicolas , Schäfer Markus
Published: March 2023

Gastric adenocarcinoma remains associated with a poor long-term survival, despite recent therapeutical advances. In most parts of the world where systematic screening programs do not exist, diagnosis is often made at advanced stages, affecting long-term prognosis. In recent years, there is increasing evidence that a large bundle of factors, ranging from the tumor microenvironment to patient ethnicity and variations in therapeutic strategy, play an important role in patient outcome. A more thorough understanding of these multi-faceted parameters is needed in order to provide a better assessment of long-term prognosis in these patients, which probably also require the refinement of current staging systems. This study aims to review existing knowledge on the clinical, biomolecular and treatment-related parameters that have some prognostic value in patients with gastric adenocarcinoma.
Predictive Biomarkers for Checkpoint Inhibitor Immune-Related Adverse Events
Authors: Les Iñigo , Martínez Mireia , Pérez-Francisco Inés , Cabero María , Teijeira Lucía , Arrazubi Virginia , Torrego Nuria , Campillo-Calatayud Ana , Elejalde Iñaki , Kochan Grazyna , Escors David
Published: March 2023

Immune-checkpoint inhibitors (ICIs) are antagonists of inhibitory receptors in the immune system, such as the cytotoxic T-lymphocyte-associated antigen-4, the programmed cell death protein-1 and its ligand PD-L1, and they are increasingly used in cancer treatment. By blocking certain suppressive pathways, ICIs promote T-cell activation and antitumor activity but may induce so-called immune-related adverse events (irAEs), which mimic traditional autoimmune disorders. With the approval of more ICIs, irAE prediction has become a key factor in improving patient survival and quality of life. Several biomarkers have been described as potential irAE predictors, some of them are already available for clinical use and others are under development; examples include circulating blood cell counts and ratios, T-cell expansion and diversification, cytokines, autoantibodies and autoantigens, serum and other biological fluid proteins, human leucocyte antigen genotypes, genetic variations and gene profiles, microRNAs, and the gastrointestinal microbiome. Nevertheless, it is difficult to generalize the application of irAE biomarkers based on the current evidence because most studies have been retrospective, time-limited and restricted to a specific type of cancer, irAE or ICI. Long-term prospective cohorts and real-life studies are needed to assess the predictive capacity of different potential irAE biomarkers, regardless of the ICI type, organ involved or cancer site.
DNA Methylation and Prospects for Predicting the Therapeutic Effect of Neoadjuvant Chemotherapy for Triple-Negative and Luminal B Breast Cancer
Authors: Sigin Vladimir O. , Kalinkin Alexey I. , Nikolaeva Alexandra F. , Ignatova Ekaterina O. , Kuznetsova Ekaterina B. , Chesnokova Galina G. , Litviakov Nikolai V. , Tsyganov Matvey M. , Ibragimova Marina K. , Vinogradov Ilya I. , Vinogradov Maxim I. , Vinogradov Igor Y. , Zaletaev Dmitry V. , Nemtsova Marina V. , Kutsev Sergey I. , Tanas Alexander S. , Strelnikov Vladimir V.
Published: March 2023

Despite advances in the diagnosis and treatment of breast cancer (BC), the main cause of deaths is resistance to existing therapies. An approach to improve the effectiveness of therapy in patients with aggressive BC subtypes is neoadjuvant chemotherapy (NACT). Yet, the response to NACT for aggressive subtypes is less than 65% according to large clinical trials. An obvious fact is the lack of biomarkers predicting the therapeutic effect of NACT. In a search for epigenetic markers, we performed genome-wide differential methylation screening by XmaI-RRBS in cohorts of NACT responders and nonresponders, for triple-negative (TN) and luminal B tumors. The predictive potential of the most discriminative loci was further assessed in independent cohorts by methylation-sensitive restriction enzyme quantitative PCR (MSRE-qPCR), a promising method for the implementation of DNA methylation markers in diagnostic laboratories. The selected most informative individual markers were combined into panels demonstrating cvAUC = 0.83 (TMEM132D and MYO15B markers panel) for TN tumors and cvAUC = 0.76 (TTC34, LTBR and CLEC14A) for luminal B tumors. The combination of methylation markers with clinical features that correlate with NACT effect (clinical stage for TN and lymph node status for luminal B tumors) produces better classifiers, with cvAUC = 0.87 for TN tumors and cvAUC = 0.83 for luminal B tumors. Thus, clinical characteristics predictive of NACT response are independently additive to the epigenetic classifier and in combination improve prediction.
Phase I Trial of [99mTc]Tc-maSSS-PEG2-RM26, a Bombesin Analogue Antagonistic to Gastrin-Releasing Peptide Receptors (GRPRs), for SPECT Imaging of GRPR Expression in Malignant Tumors
Authors: Chernov Vladimir , Rybina Anastasiya , Zelchan Roman , Medvedeva Anna , Bragina Olga , Lushnikova Nadejda , Doroshenko Artem , Usynin Evgeniy , Tashireva Liubov , Vtorushin Sergey , Abouzayed Ayman , Rinne Sara S. , Sörensen Jens , Tolmachev Vladimir , Orlova Anna
Published: March 2023

The gastrin-releasing peptide receptor (GRPR) is overexpressed in prostate cancer (PCa) and in hormone-driven breast cancer (BCa). The aim of this phase I clinical trial was to evaluate safety, biodistribution, and dosimetry after the administration of the recently developed GRPR-targeting antagonistic bombesin analogue [99mTc]Tc-maSSS-PEG2-RM26 in PCa and BCa patients. Planar and whole-body SPECT/CT imaging was performed in six PCa patients and seven BCa patients 2, 4, 6, and 24 h post the intravenous administration of 40 µg of [99mTc]Tc-maSSS-PEG2-RM26 (600–700 MBq). No adverse events or pathological changes were observed. The rapid blood clearance of [99mTc]Tc-maSSS-PEG2-RM26 was observed with predominantly hepatobiliary excretion. The effective doses were 0.0053 ± 0.0007 for male patients and 0.008 ± 0.003 mSv/MBq for female patients. The accumulation of [99mTc]Tc-maSSS-PEG2-RM26 in tumors was observed in four out of six PCa and in seven out of seven BCa patients. In four BCa patients, a high uptake of the agent into the axillary lymph nodes was detected. Immunohistochemistry revealed positive GRPR expression in 60% of primary PCa, 71.4% of BCa tumors, and 50% of examined BCa lymph nodes. In conclusion, a single administration of [99mTc]Tc-maSSS-PEG2-RM26 was safe and well tolerated. [99mTc]Tc-maSSS-PEG2-RM26 SPECT may be useful for tumor detection in PCa and BCa patients, pending further studies.