bluuebunny/crossref_metadata_2025 huggingface dataset:
Epidemiology, Etiology, and Pathophysiology of Pulmonary Embolism
Authors: Glazier Catherine R. , Baciewicz Frank A.
Published: April 2024

Over the last 20 years, there has been a progressive increase in the incidence of pulmonary embolism (PE) diagnosis in the United States, Europe, and Australia. Increased use of computed tomography pulmonary angiography has likely contributed in part to this rising incidence. However, it is pertinent to note that the burden of comorbidities associated with PE, such as malignancy, obesity, and advanced age, has also increased over the past 20 years. Time-trend analysis in North American, European, and Asian populations suggests that mortality rates associated with PE have been declining. The reported improved survival rates in PE over the past 20 years are likely, at least in part, to be the result of better adherence to guidelines, improved risk stratification, and enhanced treatment. Factors contributing to the development of venous thromboembolism (VTE) include stasis of blood, hypercoagulability, endothelial injury, and inflammation. In 70 to 80% of cases of PE, the thrombi embolizes from the proximal deep veins of the lower extremities and pelvis. Strong risk factors for VTE include lower extremity fractures and surgeries, major trauma, and hospitalization within the previous 3 months for acute myocardial infarction or heart failure with atrial fibrillation. Acute PE causes several pathophysiological responses including hypoxemia and right ventricle (RV) failure. The latter is a result of pulmonary artery occlusion and associated vasoconstriction. Hemodynamic compromise from RV failure is the principal cause of poor outcome in patients with acute PE.
Evaluation of Improved Imaging Properties with Tungsten-Based Parallel-Hole Collimators: A Monte Carlo Study
Authors: Pirayesh Islamian Jalil , Ljungberg Michael
Published: April 2024

Objectives The purpose of a parallel-hole collimator in a scintillation camera system is to transmit only those photons that have an emission angle close to the direction of the hole. This makes it possible to receive spatial information about the origin of the emission, that is, radioactivity decay. The dimension, shape, and intrahole thickness determine the spatial resolution and, by a tradeoff, sensitivity. The composition of the collimator material also plays an important role in determining a proper collimator. In this study, we compared tungsten alloys as a potential collimator material replacement for the conventional lead antimony material used in most of the current camera systems.

Materials and Methods Monte Carlo simulations of a commercial scintillation camera system with low energy high resolution (LEHR), medium-energy (ME), and high-energy (HE) collimators of lead, tungsten, and tungsten-based alloy were simulated for different I-131, Lu-177, I-123, and Tc-99m sources, and a Deluxe rod phantom using the SIMIND Monte Carlo code. Planar images were analyzed regarding spatial resolution, image contrast in a cold source case, and system sensitivity for each collimator configuration. The hole dimensions for the three collimators were those specified in the vendor's datasheet.

Results Using Pb, W, and tungsten alloy (Wolfmet) as collimator materials, the full width at half maximum (FWHM) measures for total counts (T) for LEHR with Tc-99m source (6.9, 6.8, and 6.8 mm), for ME with Lu-177 source (11.7, 11.5, and 11.6 mm), and for HE with I-131 (6.2, 13.1, and 13.1 mm) were obtained, and the system sensitivities were calculated as 89.9, 86.1, and 89.8 cpsT/MBq with Tc-99m source; 42.7, 17.4, and 20.9 cpsT/MBq with Lu-177 source; and 40.1, 69.7, and 77.4 cpsT/MBq with I-131 source. The collimators of tungsten and tungsten alloy (97.0% W, 1.5% Fe, 1.5% Ni) provided better spatial resolution and improved image contrast when compared with conventional lead-based collimators. This was due to lower septal penetration.

Conclusion The results suggest that development of a new set of ME and HE tungsten and tungsten alloy collimators could improve imaging of I-131, Lu-177, and I-123.
Evaluating Data-Driven Site Characterization Methods for Subsurface Modeling through Benchmarking
Authors:   American Society of Civil Engineers, Larbi Jelian
Published: April 2024

Data-driven site characterization (DDSC) methods generate three-dimensional digital models of subsurface environments
The time revolution in macromolecular crystallography
Authors: Khusainov Georgii , Standfuss Joerg , Weinert Tobias
Published: April 2024

Macromolecular crystallography has historically provided the atomic structures of proteins fundamental to cellular functions. However, the advent of cryo-electron microscopy for structure determination of large and increasingly smaller and flexible proteins signaled a paradigm shift in structural biology. The extensive structural and sequence data from crystallography and advanced sequencing techniques have been pivotal for training computational models for accurate structure prediction, unveiling the general fold of most proteins. Here, we present a perspective on the rise of time-resolved crystallography as the new frontier of macromolecular structure determination. We trace the evolution from the pioneering time-resolved crystallography methods to modern serial crystallography, highlighting the synergy between rapid detection technologies and state-of-the-art x-ray sources. These innovations are redefining our exploration of protein dynamics, with high-resolution crystallography uniquely positioned to elucidate rapid dynamic processes at ambient temperatures, thus deepening our understanding of protein functionality. We propose that the integration of dynamic structural data with machine learning advancements will unlock predictive capabilities for protein kinetics, revolutionizing dynamics like macromolecular crystallography revolutionized structural biology.
A theoretical study on optical field distribution and absorption of stacked thin films supported by a reflective back layer
Authors: Vy Nguyen Duy , Pham Vinh N. T. , Dat Le Tri
Published: April 2024

The stacked thin films have recently been of great interest for enhancing the optical and thermal absorption of the system via their specific optical properties depending on the optical wavelength and layer thickness. Here, by using Maxwell’s equation for the electromagnetic fields penetrating thin films, we simulated in detail the absorption of the nanometer-thick thin film of several materials, such as Au, Ag, Cu, and Al, and figured out the optimal thickness range for the outer layers exposed to incoming field to optimize the energy harvesting. In particular, the absorption of the film supported by a totally reflective layer at the back of the structure could be significantly enhanced, and the maximal absorption happens at a layer much thinner than that in the case of the film solely irradiated by the field. These results could help suitably choosing of detailed thickness for the structure to optimize the field effect on a specific layer.
Wireless-based portable device heart rate measurement as biomedical devices for stress detection
Authors: Kuncoro C. Bambang Dwi , Efendi Adhan , Luo Win-Jet , Sakanti Maria Mahardini , Ammarullah Muhammad Imam
Published: April 2024

Stress can increase the heart rate, causing dangerous conditions that cause significant harm and even death. Therefore, managing stress well to control the heart rate is vital. Monitoring stress levels using biomedical devices is essential to prevent further damage to the individual. Regular monitoring facilitates the acquisition of individual stress levels through heart rate information. In this research, a MAX30100 sensor can produce Beats Per Minute (BPM) data, which show the stress experienced by a person during four daily activities (sitting, walking, running, and sleeping) and when watching a horror movie. This research underscores the potential of cost-effective wireless solutions to increase portability and facilitate real-time monitoring and remote surveillance. The researchers introduced an innovative methodology by reusing everyday consumer devices, initially intended for routine activities, to measure stress levels in a unique context—precisely in a controlled experiment involving a movie-watching scenario. The ultimate achievement of this research was creating a stationary heart rate monitoring device that is easy to access, easy to use, space-saving, and adept at effectively tracking stress indicators. BPM readings obtained from the MAX30100 sensor have scores similar to those obtained from the heart rate app. The researchers proposed the inclusion of other sensors, such as a photoplethysmograph, an electrocardiogram, and galvanic skin response, to provide a more holistic assessment of stress. Leveraging cloud technology for data storage enables long-term monitoring and in-depth analysis of stress patterns over time.
Modeling and analysis of the triple diffusion unsteady flow of couple stress nanofluid with variable viscosity and distinct thermal sources
Authors: Ghachem Kaouther , Khan Sami Ullah , Safra Imen , Albalawi Hind , Labidi Taher , Kolsi Lioua
Published: April 2024

Thanks to their optimal thermal characteristics, nanomaterials stand out for their varied applications in heat transfer systems, energy storage, industrial processes, and biomedical research. Recently, scientists explored various dynamic properties in nanofluid flow to develop an even better thermal model. In this context, the phenomenon of triple diffusion in nanofluids constitutes an active area of research, offering promising applications in nanotechnology, metallurgical processes, chemical reactors, and thermo-diffusion processes. This paper analyzes the triple diffusion flow of a torque-constrained nanofluid, induced by a periodically oscillating porous surface, taking into account the importance of variations in thermal consequences. The viscosity of the torque-constrained nanofluid is assumed to be temperature-dependent. The analysis takes into account the variable role of thermal conductivity, mass diffusivity, and solute volume fraction. The modeling of the problem is expressed by coupled nonlinear partial differential equations. The semi-analytic technique, known as the homotopic analysis scheme, is used for resolution. The solution is validated and confirms the convergence region. The physical aspects of the parameters are examined with regard to the parameters involved. The simulated observations reveal that with the Dufour–Lewis factor and varying mass diffusivity, an increase in solute concentration is seen. The concentration of nanoparticles decreases with the nano-Lewis number.
Vertical distribution of edaphic oribatid mites (Acari: Oribatida) in two artificial forests planted on temperate grasslands
Authors: Levy Eugenia , Alvarez M. Fernanda , Fredes Natalia A.
Published: April 2024

Context Oribatid mites are the most abundant taxon in forest soils that rely on porosity and organic matter availability. Exotic forests of Pinus radiata and Eucalyptus globulus planted over native grasslands in the Pampas region of Argentina have produced modification in soil properties, which can affect the composition and structure of native oribatids communities. Aims To compare oribatid communities in soils under artificial woodlands of pine and eucalyptus and to assess the vertical distribution of oribatids in relation to edaphic variables. Methods In each forest, oribatids were collected from three levels of the A horizon: (1) litter layer; (2) 0–5 cm; and (3) 5–10 cm. Edaphic variables measured were penetration resistance, bulk density, total porosity, pH and water content and luminosity at soil level. Density, species richness, diversity and evenness of oribatids were calculated in each level of each forest. Comparisons were made through multivariate analyses. Key results Edaphic variables showed no significant differences between plots but litters were structurally different. Richness and diversity showed no differences between plots whereas evenness was significantly higher in plot P. Density had higher values in the litter layer of both forests. Canonical Analysis of Principal Coordinates (CAP) showed that Pine plots had a more defined vertical distribution. Conclusions The structure and composition of litters promoted differences in the vertical distribution of oribatids. In Pinus, thick unaltered litter showed a marked vertical gradient of mites along levels while in Eucalyptus, thin and easily decomposing litter, showed no vertical patterns. Implications Assessment and management of soil biodiversity in artificial woodlands.
Multiple approaches in the study of ancient human microbiome
Authors: Rosas-Plaza Santiago , Rosas-Plaza Ximena , Cerritos René
Published: April 2024

Research related to the analysis of microbial communities in ancient human samples began two decades ago. Since then, new technologies as well as new bioinformatic tools have been developed for the manipulation of massive data. All this together, with the theoretical knowledge generated from microbial ecology has expanded our understanding of the human microbiome. Despite the challenge of studying ancient samples and their microbiome, some approaches applied to the contemporary human microbiome have been successfully applied to ancient samples. Most studies in ancient samples have focused on taxonomic profiling at the community level with metagenomic or high-throughput 16S rRNA gene amplicon sequencing data. However, other approaches have emerged in order to get deeper understanding of the ancient human microbiome. Therefore, the aim of this review is to discuss all the approaches and methodologies successfully applied to ancient samples and ancient microbiome as well as the information that can be generated using them, in order to understand the evolution of human microbiome interactions throughout history.
The occurrence of Corynosoma strumosum in the grey seal, Halichoerus grypus, caught off the Atlantic coast of Ireland
Authors: O'Neill G. , Whelan J.
Published: April 2024

The occurrence, location and sex ratio of Corynosoma strumosum (Acanthoceophala: Polymorphidae) from 26 juvenile grey seals, Halichoerus grypus, by-caught from around the Inishkea Islands, Co. Mayo, from March to June 1997 were recorded. The location of C. strumosum within the seal did not vary, with all worms being found in the small intestine. The prevalence of infection was 100%, with no other acanthocephalan species being recorded. The mean abundance was 416 (range 80–846) C. strumosum per seal. There was no significant change in abundance by month. The sex ratio of mature female to mature male parasites was 1.2:1. The lengths of female and male worms did not differ significantly.
Effects of quantum noise on teleportation of arbitrary two-qubit state via five-particle Brown state
Authors: Wang 汪 Ao 澳 , Wei 魏 Yu-Zhen 玉震 , Jiang 姜 Min 敏 , Li 李 Yong-Cheng 泳成 , Chen 陈 Hong 虹 , Huang 黄 Xu 旭
Published: April 2024

We propose a new protocol for quantum teleportation (QT) which adopts the Brown state as the quantum channel. This work focuses on the teleportation of a single unknown two-qubit state via a Brown state channel in an ideal environment. To validate the effectiveness of our proposed scheme, we conduct experiments by using the quantum circuit simulator Quirk. Furthermore, we investigate the effects of four noisy channels, namely, the phase damping noise, the bit-flip noise, the amplitude damping noise, and the phase-flip noise. Notably, we employ Monte Carlo simulation to elucidate the fidelity density under various noise parameters. Our analysis demonstrates that the fidelity of the protocol in a noisy environment is influenced significantly by the amplitude of the initial state and the noise factor.
Mutation in a non-force-bearing region of protein L influences force-dependent unfolding behavior
Authors: Jiang 蒋 Huanjie 环杰 , Wang 王 Yanwei 艳伟 , Chen 陈 Jiayuan 家媛 , Hu 胡 Dan 丹 , Pan 潘 Hai 海 , Guo 郭 Zilong 子龙 , Chen 陈 Hu 虎
Published: April 2024

Single-molecule magnetic tweezers (MTs) have revealed multiple transition barriers along the unfolding pathway of several two-state proteins, such as GB1 and Csp. In this study, we utilized MTs to measure the force-dependent folding and unfolding rates of both protein L (PLWT) and its Y47W mutant (PLY47W) where the mutation point is not at the force-bearing β-strands. The measurements were conducted within a force range of 3–120 pN. Notably, the unfolding rates of both PLWT and PWY47W exhibit distinct force sensitivities below 50 pN and above 60 pN, implying a two-barrier free energy landscape. Both PLWT and PLY47W share the same force-dependent folding rate and the same transition barriers, but the unfolding rate of PLY47W is faster than that of PLWT. Our finding demonstrates that the residue outside of the force-bearing region will also affect the force-induced unfolding dynamics.
Goodenough–Kanamori–Anderson high-temperature ferromagnetism in tetragonal transition-metal xenes
Authors: Yorulmaz U , Šabani D , Sevik C , Milošević M V
Published: April 2024

Seminal Goodenough–Kanamori–Anderson (GKA) rules provide an inceptive understanding of the superexchange interaction of two magnetic metal ions bridged with an anion, and suggest fostered ferromagnetic interaction for orthogonal bridging bonds. However, there are no examples of two-dimensional (2D) materials with structure that optimizes the GKA arguments towards enhanced ferromagnetism and its critical temperature. Here we reveal that an ideally planar GKA ferromagnetism is indeed stable in selected tetragonal transition-metal xenes (tTMXs), with Curie temperature above 300 K found in CrC and MnC. We provide the general orbitally-resolved analysis of magnetic interactions that supports the claims and sheds light at the mechanisms dominating the magnetic exchange process in these structures. Furthermore, we propose the set of three GKA-like rules that will guarantee room temperature ferromagetnism. With recent advent of epitaxially-grown tetragonal 2D materials, our findings earmark tTMXs for facilitated spintronic and magnonic applications, or as a desirable magnetic constituent of functional 2D heterostructures.
Experimental study of Yushania alpina bamboo fiber
Authors: Asres Muluken Awulachew , Zerayohannes Girma , Zekaria Adil , Nuramo Denamo Addissie
Published: April 2024

The characteristics of bamboo fiber depend on the source species. This study investigated the properties of Yushania alpina bamboo fibers extracted using mechanical, chemical, and combined methods. Samples from each extraction method were tested for tensile strength. Scanning electron microscopy was used to examine the morphology of the fibers. Fourier transform infrared was used to trace functional group changes. The absorption capacity of the fibers was also examined. The thermal properties of the fibers were investigated using thermogravimetric analysis. The chemical compositions of the fibers were studied using a gravimetric method. In contrast to mechanically and combinedly extracted bamboo fibers, chemically extracted fiber had up to 90.84% and 67.06% increments in tensile strength, respectively. Scanning electron microscopy revealed the removal of attachments on the surfaces of the fibers extracted chemically. The diameter of the fibers extracted chemically was reduced. Fourier transform infrared showed no change in functional groups among the extracted fibers. However, lignin content was reduced in chemically extracted fibers. The absorption capacity of the fibers was encouraging for use in composites. Thermal analysis showed improved thermal properties with the chemical method. Chemical analysis revealed reduced lignin and hemicellulose compositions in chemically extracted fibers. This study suggests bamboo fibers can be used in the construction industry for sustainability.
Optimizing scan time and bayesian penalized likelihood reconstruction algorithm in copper-64 PET/CT imaging: a phantom study
Authors: Monsef Abbas , Sheikhzadeh Peyman , Steiner Joseph R , Sadeghi Fatemeh , Yazdani Mohammadreza , Ghafarian Pardis
Published: April 2024

Objectives: The aim of this study was to evaluate Cu-64 PET phantom image quality using Bayesian Penalized Likelihood (BPL) and Ordered Subset Expectation Maximum with point-spread function modeling (OSEM-PSF) reconstruction algorithms. In the BPL, the regularization parameter

β

was varied to identify the optimum value for image quality. In the OSEM-PSF, the effect of acquisition time was evaluated to assess the feasibility of shortened scan duration. Methods: A NEMA IEC PET body phantom was filled with known activities of water soluble Cu-64. The phantom was imaged on a PET/CT scanner and was reconstructed using BPL and OSEM-PSF algorithms. For the BPL reconstruction, various

β

values (150, 250, 350, 450, and 550) were evaluated. For the OSEM-PSF algorithm, reconstructions were performed using list-mode data intervals ranging from 7.5 to 240 s. Image quality was assessed by evaluating the signal to noise ratio (SNR), contrast to noise ratio (CNR), and background variability (BV). Results: The SNR and CNR were higher in images reconstructed with BPL compared to OSEM-PSF. Both the SNR and CNR increased with increasing β, peaking at

β

= 550. The CNR for all β, sphere sizes and tumor-to-background ratios (TBRs) satisfied the Rose criterion for image detectability (CNR > 5). BPL reconstructed images with β = 550 demonstrated the highest improvement in image quality. For OSEM-PSF reconstructed images with list-mode data duration ≥ 120 s, the noise level and CNR were not significantly different from the baseline 240 s list-mode data duration. Conclusions: BPL reconstruction improved Cu-64 PET phantom image quality by increasing SNR and CNR relative to OSEM-PSF reconstruction. Additionally, this study demonstrated scan time can be reduced from 240 to 120 s when using OSEM-PSF reconstruction while maintaining similar image quality. This study provides baseline data that may guide future studies aimed to improve clinical Cu-64 imaging.
Marijuana Use Increases Risk of Hematoma Formation in Patients Undergoing Abdominal Body Contouring Surgery
Authors: Rudy Hayeem , Lu Yi-Hsueh , Chernovolenko Daniel , Grande Julia , Park Michelle , Ricci Joseph
Published: April 2024

Goals/Purpose

The physiological effect of marijuana is thought to include platelet inhibition and poor wound healing, however, there is mixed clinical evidence in the plastic surgery literature regarding this topic. As marijuana usage continues to increase, this study sought to identify the effect of marijuana on postoperative complication rates in patients undergoing abdominal body contouring surgery (ABCS) while controlling for tobacco consumption and other comorbidities.

Methods/Technique

A retrospective cohort study was conducted in patients who underwent panniculectomy or abdominoplasty at our institution between 2016 and 2021. Patients were separated into groups of active (at time of surgery), former, and no marijuana use. Demographic characteristics, smoking history, laboratory results, comorbidities, operative details, and postoperative complications including hematoma, deep vein thrombosis (DVT) and pulmonary embolism (PE), and wound healing complications were analyzed. Parametric, nonparametric, and multivariable regression modeling was used for analysis.

Results/Complications

815 patients who underwent panniculectomy or abdominoplasty were included in the study. 61 patients (7.5%) reported active marijuana use at time of their surgery, and 31 patients (3.8%) reported former marijuana use (defined as last use >2 months prior). Patients who reported any marijuana use were significantly younger (40.5 years vs 45.9 years; p<0.0001) and more likely to identify as Black (OR=2.34; p=0.005). Any marijuana use was significantly associated with reported tobacco use (OR=4.80; p<0.001; 95% CI 1.69-12.69) After adjusting for age, BMI, tobacco use, platelet count, and comorbidity index, active marijuana use was associated with significantly higher risk of hematoma formation (OR=2.55; P= 0.03; 95% CI 1.12-6.55) as well as any complication combined (OR=1.73; p=0.02, 95% CI 1.15-3.56). Other complications, including venous thromboembolism, infection, seroma, umbilical necrosis, wound dehiscence, or anesthetic-related complications were not significantly associated with reported marijuana use.

Conclusion

When controlling for multiple confounders, marijuana use appears to be independently associated with increased risk of hematoma development in patients undergoing abdominal body contouring surgery with either abdominoplasty or panniculectomy. Further research is warranted to investigate the exact relationships and mechanisms of action behind this finding.
Prognostic implication of lung ultrasound in heart failure: a pooled analysis of international cohorts
Authors: Rastogi Tripti , Gargani Luna , Pellicori Pierpaolo , Lamiral Zohra , Ambrosio Giuseppe , Bayés-Genis Antoni , Domingo Mar , Lupon Josep , Simonovic Dejan , Pugliese Nicola Riccardo , Ruocco Gaetano , Duarte Kevin , Coiro Stefano , Palazzuoli Alberto , Girerd Nicolas
Published: April 2024

Aims

Lung ultrasound (LUS) is often used to assess congestion in heart failure (HF). In this study, we assessed the prognostic role of LUS in patients with HF at admission and hospital discharge, and in an outpatient setting, and explored whether clinical factors [age, sex, left ventricular ejection fraction (LVEF), and atrial fibrillation] impact the prognostic value of LUS findings. Further, we assessed the incremental prognostic value of LUS on top of the following two clinical risk scores: (i) the atrial fibrillation, haemoglobin, elderly, abnormal renal parameters, diabetes mellitus (AHEAD) and (ii) the Meta-Analysis Global Group in Chronic Heart Failure (MAGGIC) clinical risk scores.

Methods and results

We pooled data on patients hospitalized for HF or followed up in outpatient clinics from international cohorts. We enrolled 1947 patients at admission (n = 578), discharge (n = 389), and in outpatient clinics (n = 980). The total LUS B-line count was calculated for the eight-zone scanning protocol. The primary outcome was a composite of rehospitalization for HF and all-cause death. Compared with those in the lower tertiles of B lines, patients in the highest tertiles were older, more likely to have signs of HF and had higher N-terminal pro b-type natriuretic peptide (NT-proBNP) levels. A higher number of B lines was associated with increased risk of primary outcome at discharge [Tertile 3 vs. Tertile 1: adjusted hazard ratio (HR): 5.74 (3.26–10.12), P < 0.0001] and in outpatients [Tertile 3 vs. Tertile 1: adjusted HR: 2.66 (1.08–6.54), P = 0.033]. Age and LVEF did not influence the prognostic capacity of LUS in different clinical settings. Adding B-line count to the MAGGIC and AHEAD scores improved net reclassification significantly in all three clinical settings.

Conclusion

A higher number of B lines in patients with HF was associated with an increased risk of morbidity and mortality, regardless of the clinical setting.
Calcification of surgical aortic bioprostheses and its impact on clinical outcome
Authors: Guimbretière Guillaume , Sénage Thomas , Boureau Anne-Sophie , Roos Jean-Charles , Bernard Quentin , Carlier Baptiste , Veziers Joelle , Cueff Caroline , Piriou Nicolas , Coste Guenola , Fellah Imen , Lelarge Coline , Capoulade Romain , Jaafar Philippe , Manigold Thibaud , Letocart Vincent , Warin-Fresse Karine , Guérin Patrice , Costa Cristina , Vadori Marta , Galinañes Manuel , Manez Rafael , Soulillou Jean-Paul , Cozzi Emanuele , Padler-Karavani Vered , Serfaty Jean-Michel , Roussel Jean-Christian , Le Tourneau Thierry
Published: April 2024

Aims

Aortic valve calcification (AVC) of surgical valve bioprostheses (BPs) has been poorly explored. We aimed to evaluate in vivo and ex vivo BP AVCs and its prognosis value.

Methods and results

Between 2011 and 2019, AVC was assessed using in vivo computed tomography (CT) in 361 patients who had undergone surgical valve replacement 6.4 ± 4.3 years earlier. Ex vivo CT scans were performed for 37 explanted BPs. The in vivo CT scans were interpretable for 342 patients (19 patients [5.2%] were excluded). These patients were 77.2 ± 9.1 years old, and 64.3% were male. Mean in vivo AVC was 307 ± 500 Agatston units (AU). The AVC was 562 ± 570 AU for the 183 (53.5%) patients with structural valve degeneration (SVD) and 13 ± 43 AU for those without SVD (P < 0.0001). In vivo and ex vivo AVCs were strongly correlated (r = 0.88, P < 0.0001). An in vivo AVC > 100 AU (n = 147, 43%) had a specificity of 96% for diagnosing Stage 2–3 SVD (area under the curve = 0.92). Patients with AVC > 100 AU had a worse outcome compared with those with AVC ≤ 100 AU (n = 195). In multivariable analysis, AVC was a predictor of overall mortality (hazard ratio [HR] and 95% confidence interval = 1.16 [1.04–1.29]; P = 0.006), cardiovascular mortality (HR = 1.22 [1.04–1.43]; P = 0.013), cardiovascular events (HR = 1.28 [1.16–1.41]; P < 0.0001), and re-intervention (HR = 1.15 [1.06–1.25]; P < 0.0001). After adjustment for Stage 2–3 SVD diagnosis, AVC remained a predictor of overall mortality (HR = 1.20 [1.04–1.39]; P = 0.015) and cardiovascular events (HR = 1.25 [1.09–1.43]; P = 0.001).

Conclusion

CT scan is a reliable tool to assess BP leaflet calcification. An AVC > 100 AU is tightly associated with SVD and it is a strong predictor of overall mortality and cardiovascular events.
A de novo genome assembly of Solanum bulbocastanum Dun., a Mexican diploid species reproductively isolated from the A-genome species, including cultivated potatoes
Authors: Hosaka Awie J , Sanetomo Rena , Hosaka Kazuyoshi
Published: April 2024

Potato and its wild relatives are distributed mainly in the Mexican highlands and central Andes of South America. The South American A-genome species, including cultivated potatoes, are reproductively isolated from Mexican diploid species. Whole-genome sequencing has disclosed genome structure and similarity, mostly in cultivated potatoes and their closely related species. In this study, we generated a chromosome-scale assembly of the genome of a Mexican diploid species, Solanum bulbocastanum Dun., using PacBio long-read sequencing, optical mapping, and Hi-C scaffolding technologies. The final sequence assembly consisted of 737.9 Mb, among which 647.0 Mb were anchored to the 12 chromosomes. Compared with chromosome-scale assemblies of S. lycopersicum (tomato), S. etuberosum (non-tuber-bearing species with E-genome), S. verrucosum, S. chacoense, S. multidissectum, and S. phureja (all four are A-genome species), the S. bulbocastnum genome was the shortest. It contained fewer transposable elements (56.2%) than A-genome species. A cluster analysis was performed based on pairwise ratios of syntenic regions among the seven chromosome-scale assemblies, showing that the A-genome species were first clustered as a distinct group. Then, this group was clustered with S. bulbocastanum. Sequence similarity in 1,624 single-copy orthologous gene groups among 36 Solanum species and clones separated S. bulbocastanum as a specific group, including other Mexican diploid species, from the A-genome species. Therefore, the S. bulbocastanum genome differs in genome structure and gene sequences from the A-genome species. These findings provide important insights into understanding and utilizing the genetic diversity of S. bulbocastanum and the other Mexican diploid species in potato breeding.
Noninvasive tests for liver fibrosis in 2024: are there different scales for different diseases?
Authors: Lai Jimmy Che-To , Liang Lilian Yan , Wong Grace Lai-Hung
Published: April 2024

Liver fibrosis is the common pathway from various chronic liver diseases and its progression leads to cirrhosis which carries a significant risk for the development of portal hypertension-related complications and hepatocellular carcinoma. It is crucial to identify and halt the worsening of liver fibrosis given its important prognostic implication. Liver biopsy is the gold standard for assessing the degree of liver fibrosis but is limited due to its invasiveness and impracticality for serial monitoring. Many noninvasive tests have been developed over the years trying to assess liver fibrosis in a practical and accurate way. The tests are mainly laboratory- or imaging-based, or in combination. Laboratory-based tests can be derived from simply routine blood tests to patented laboratory parameters. Imaging modalities include ultrasound and magnetic resonance elastography, in which vibration-controlled transient elastography is the most widely validated and adopted whereas magnetic resonance elastography has been proven the most accurate liver fibrosis assessment tool. Nonetheless, noninvasive tests do not always apply to all liver diseases, nor does a common cut-off value of a test mean the same degree of liver fibrosis in different scenarios. In this review, we discuss the diagnostic and prognostic performance, as well as the confounders and limitations, of different noninvasive tests on liver fibrosis assessment in various liver diseases.
Streamlining remote nanopore data access with slow5curl
Authors: Wong Bonson , Ferguson James M , Do Jessica Y , Gamaarachchi Hasindu , Deveson Ira W
Published: April 2024

Background

As adoption of nanopore sequencing technology continues to advance, the need to maintain large volumes of raw current signal data for reanalysis with updated algorithms is a growing challenge. Here we introduce slow5curl, a software package designed to streamline nanopore data sharing, accessibility, and reanalysis.

Results

Slow5curl allows a user to fetch a specified read or group of reads from a raw nanopore dataset stored on a remote server, such as a public data repository, without downloading the entire file. Slow5curl uses an index to quickly fetch specific reads from a large dataset in SLOW5/BLOW5 format and highly parallelized data access requests to maximize download speeds. Using all public nanopore data from the Human Pangenome Reference Consortium (>22 TB), we demonstrate how slow5curl can be used to quickly fetch and reanalyze raw signal reads corresponding to a set of target genes from each individual in large cohort dataset (n = 91), minimizing the time, egress costs, and local storage requirements for their reanalysis.

Conclusions

We provide slow5curl as a free, open-source package that will reduce frictions in data sharing for the nanopore community: https://github.com/BonsonW/slow5curl.
Colorimetric Concurrent Determination of Ultra-Trace Amounts of Pilocarpine and Timolol as Anti-Glaucoma Drugs in Binary Mixtures Using a Multivariate Calibration Approach Based on the Aggregation of Gold Nanoparticles
Authors: Zanjani Asma , Sohrabi Mahmoud Reza , Kabiri Fard Hassan
Published: April 2024

Background

To study the ultra-trace simultaneous determination of drugs, the colorimetric method in combination with chemometrics can be used.

Objective

In this study, a simple, rapid, and sensitive UV-Vis spectrophotometric method using gold nanoparticles (AuNPs) was introduced for the simultaneous determination of ultra-trace amounts of pilocarpine (PIL) and timolol (TIM) in binary mixtures and biological samples.

Methods

AuNPs interacted with components and the aggregation mode of NPs occurred, and, finally, the color change of the solution (red to gray) was observed with the naked eye without the most modern and expensive instruments. The characterization of AuNPs was evaluated by transmission electron microscopy (TEM) and dynamic light scattering (DLS).

Results

The validation of the colorimetric way was studied in the concentration range of 100–800 and 100–600 μg/L with good linearity equal to 0.9772 and 0.9891 for PIL and TIM, respectively. The limit of detection (LOD) was found to be 165.00 and 92.40 μg/L, where the limit of quantitation (LOQ) was 500.00 and 280.00 μg/L for PIL and TIM, respectively. The effect of some factors such as interaction time, the concentration of components, and the volume of buffer on absorbance was investigated. Partial least squares (PLS) as an efficient multivariate calibration method was combined with colorimetry for the simultaneous determination of PIL and TIM in binary mixtures. The optimum number of latent variables was selected by k-fold cross-validation based on minimum mean square error prediction (MSEP), and the number of components equal to 1 with MSEP of 1.085 and 0.763 was considered for PIL and TIM, respectively. The mean recovery was obtained at 100.20 and 101.55% for PIL and TIM, respectively.

Conclusions

The colorimetric method can be introduced as a proper option for the simultaneous determination of components in pharmaceutical formulations and other samples.

Highlights

A colorimetric method using AuNPs was proposed. The PLS method was coupled with a colorimetric method for the ultra-trace simultaneous estimation of PIL and TIM in binary mixtures. Ultra-trace amounts of PIL and TIM were also determined in biological samples. The proposed method is simple, fast, and less expensive than chromatography methods.
Morphological variation of the cirri and penis in the hermaphroditic sea-turtle barnacle Chelonibia testudinaria (Linnaeus, 1758) (Cirripedia: Balanomorpha: Chelonibiidae)
Authors: Cash Kevin , Goodwin Glenn D , Burkholder Derek , Hoch J Matthew
Published: April 2024

Phenotypic plasticity of the penis and cirri of acorn barnacles is well documented for intertidal species. We investigated whether these phenomena also occur in the epizoic sea-turtle barnacle, Chelonibia testudinaria (Linnaeus, 1758). We compared the morphologies of the penis and cirri, and fertilization rates for adult, hermaphroditic barnacles on different regions of the carapace of sea turtles, and at different levels of crowding on the carapace. Barnacles were more abundant in the posterior position of loggerhead sea turtles, Caretta caretta (Linnaeus, 1758), followed by the central, central costal, and anterior positions. Barnacles in clustered groups were fertilized 68% more often than solitary individuals. Penis size increased with barnacle size and among individual sea turtles. Cirrus length and width also varied with barnacle size, among individual sea turtles, and among positions. Larger barnacles had longer and wider feeding cirri, and cirri were longer and wider in the central and posterior positions of the carapace than in the anterior and central costal positions. The phenotypic plasticity of the morphologies of the penis and cirrus is far less dramatic in Ch. testudinaria than in intertidal species. This may be explained by the epizoic barnacles not being permanently fixed and able to move to preferred positions on the host shell.
Nurse bees regulate the larval nutrition of developing workers (Apis mellifera) when feeding on various pollen types
Authors: Kratz Madlen , Manning Robert , Dods Kenneth , Baer Boris , Blache Dominique
Published: April 2024

Nutrition has been identified as a key driver of colony health and productivity. Yet, in honey bees, relatively little is known about how the vast variety of natural pollen sources impact larval development. The impact of the nutritional quality of 4 naturally occurring pollen sources, of importance to the Western Australian beekeeping industry, was tested on honey bee (Apis mellifera L.) development. Bee packages consisting of 800 g of bees and a mated sister queen were assigned to 40 nucleus hives and randomly allocated to one of the 4 feed treatments (10 colonies each) of marri (Corymbia calophylla Lindl.), jarrah (Eucalyptus marginata Sm.), clover (Trifolium repens L.), and canola (Brassica napus L.) pollen. Emerging bees were collected once the first bees started hatching on the assigned feed sources. Newly emerging bees were weighed individually, and body composition was measured in batches according to the feed treatment groups. Food consumption was recorded for the duration of the experiment. Nurse bees successfully raised young adult workers from the larval stage until emergence when fed with one of 4 pollen patties with different nutritional qualities. There was no difference in the body composition or weight of emerging bees fed on the different pollen types. However, the body weight of bees increased over time, most likely related to colony size and structure. With the type of pollen patties having little impact on larval development, the availability of pollen may be more important than its composition, providing bees have access to all essential nutrients.
Behavior of an aerodynamic sliding bearing with textured surface under turbulent conditions
Authors: Kadda Mehala , Nadia Bendaoud , Zohra Khelif Fatima , Habib Bendaoud Mohammed , Imene Bouchelaghem , Hadja Khoussa
Published: April 2024

Recent advancements in turbomachinery development aim to reduce components while improving performance and minimizing environmental impact. Aerodynamic bearings, supporting high-speed rotors by dissipating significant energies, are key elements requiring thorough understanding. In order to better predict the behavior of aerodynamic bearings operating under severe conditions, numerical models using computational fluid dynamics have been employed to study the thermal effect on the tribological behavior of these air-lubricated bearings. An analysis of surface texturing of the bearings has also been conducted to evaluate its influence on operational performance compared to non-textured surfaces, considering the influence of rotation speed, radial load, and textures on the tribological performance of plain bearings. The main results observed are as follows. First, there is a noticeable change in geometric characteristics, such as the application of micro-textures, lubrication and friction, compared with conventional plain bearings. This textured surface appears to have a significant influence on the pressure and velocity distribution of the lubricating fluid, leading to significant changes in the bearing's tribological and operational performance. In addition, numerical analysis also reveals significant variations in shear stresses in the vicinity of the bearing walls. These variations can potentially affect the strength and durability of the bearing under severe operating conditions. Additionally, the results show a downward trend in system temperature, suggesting improved thermal management thanks to the textured surface. Another essential aspect revealed by this analysis is the decrease in the coefficient of friction with increasing shaft speed. This observation underlines the importance of operating speed and applied radial load in the bearing's tribological behavior and suggests further optimization possibilities for reducing energy losses and extending the life of the aerodynamic sliding bearing.
NF1 mutation-driven neuronal hyperexcitability sets a threshold for tumorigenesis and therapeutic targeting of murine optic glioma
Authors: Anastasaki Corina , Chatterjee Jit , Koleske Joshua P , Gao Yunqing , Bozeman Stephanie L , Kernan Chloe M , Marco Y Marquez Lara I , Chen Ji-Kang , Kelly Caitlin E , Blair Connor J , Dietzen Dennis J , Kesterson Robert A , Gutmann David H
Published: April 2024

Background

With the recognition that noncancerous cells function as critical regulators of brain tumor growth, we recently demonstrated that neurons drive low-grade glioma initiation and progression. Using mouse models of neurofibromatosis type 1 (NF1)-associated optic pathway glioma (OPG), we showed that Nf1 mutation induces neuronal hyperexcitability and midkine expression, which activates an immune axis to support tumor growth, such that high-dose lamotrigine treatment reduces Nf1-OPG proliferation. Herein, we execute a series of complementary experiments to address several key knowledge gaps relevant to future clinical translation.

Methods

We leverage a collection of Nf1-mutant mice that spontaneously develop OPGs to alter both germline and retinal neuron-specific midkine expression. Nf1-mutant mice harboring several different NF1 patient-derived germline mutations were employed to evaluate neuronal excitability and midkine expression. Two distinct Nf1-OPG preclinical mouse models were used to assess lamotrigine effects on tumor progression and growth in vivo.

Results

We establish that neuronal midkine is both necessary and sufficient for Nf1-OPG growth, demonstrating an obligate relationship between germline Nf1 mutation, neuronal excitability, midkine production, and Nf1-OPG proliferation. We show anti-epileptic drug (lamotrigine) specificity in suppressing neuronal midkine production. Relevant to clinical translation, lamotrigine prevents Nf1-OPG progression and suppresses the growth of existing tumors for months following drug cessation. Importantly, lamotrigine abrogates tumor growth in two Nf1-OPG strains using pediatric epilepsy clinical dosing.

Conclusions

Together, these findings establish midkine and neuronal hyperexcitability as targetable drivers of Nf1-OPG growth and support the use of lamotrigine as a potential chemoprevention or chemotherapy agent for children with NF1-OPG.
Nasal septum-derived chondroprogenitor cells control mandibular condylar resorption consequent to orthognathic surgery: a clinical trial
Authors: Tesch Ricardo de Souza , Takamori Esther Rieko , Menezes Karla , Carias Rosana Bizon Vieira , Rebelatto Carmen Lucia Kuniyoshi , Senegaglia Alexandra Cristina , Daga Debora Regina , Fracaro Leticia , Robert Anny Waloski , Pinheiro Carlos Bruno Reis , Aguiar Marcelo de Freitas , Blanco Pablo Javier , Zilves Eduardo Guerreiro , Brofman Paulo Roberto Slud , Borojevic Radovan
Published: April 2024

Condylar resorption is an aggressive and disability form of temporomandibular joint (TMJ) degenerative disease, usually non-respondent to conservative or minimally invasive therapies and often leading to surgical intervention and prostheses implantation. This condition is also one of the most dreaded postoperative complications of orthognathic surgery, with severe cartilage erosion and loss of subchondral bone volume and mineral density, associated with a painful or not inflammatory processes. Because regenerative medicine has emerged as an alternative for orthopedic cases with advanced degenerative joint disease, we conducted a phase I/IIa clinical trial (U1111-1194-6997) to evaluate the safety and efficacy of autologous nasal septal chondroprogenitor cells. Ten participants underwent biopsy of the nasal septum cartilage during their orthognathic surgery. The harvested cells were cultured in vitro and analyzed for viability, presence of phenotype markers for mesenchymal stem and/or chondroprogenitor cells, and the potential to differentiate into chondrocytes, adipocytes, and osteoblasts. After the intra-articular injection of the cell therapy, clinical follow-up was performed using the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) and computed tomography (CT) images. No serious adverse events related to the cell therapy injection were observed during the 12-month follow-up period. It was found that autologous chondroprogenitors reduced arthralgia, promoted stabilization of mandibular function and condylar volume, and regeneration of condylar tissues. This study demonstrates that chondroprogenitor cells from the nasal septum may be a promise strategy for the treatment of temporomandibular degenerative joint disease that do not respond to other conservative therapies.