bluuebunny/crossref_metadata_2025 huggingface dataset:
A Recap of Heme Metabolism towards Understanding Protoporphyrin IX Selectivity in Cancer Cells
Authors: Kiening Martin , Lange Norbert
Published: July 2022

Mitochondria are essential organelles of mammalian cells, often emphasized for their function in energy production, iron metabolism and apoptosis as well as heme synthesis. The heme is an iron-loaded porphyrin behaving as a prosthetic group by its interactions with a wide variety of proteins. These complexes are termed hemoproteins and are usually vital to the whole cell comportment, such as the proteins hemoglobin, myoglobin or cytochromes, but also enzymes such as catalase and peroxidases. The building block of porphyrins is the 5-aminolevulinic acid, whose exogenous administration is able to stimulate the entire heme biosynthesis route. In neoplastic cells, this methodology repeatedly demonstrated an accumulation of the ultimate heme precursor, the fluorescent protoporphyrin IX photosensitizer, rather than in healthy tissues. While manifold players have been proposed, numerous discrepancies between research studies still dispute the mechanisms underlying this selective phenomenon that yet requires intensive investigations. In particular, we wonder what are the respective involvements of enzymes and transporters in protoporphyrin IX accretion. Is this mainly due to a boost in protoporphyrin IX anabolism along with a drop of its catabolism, or are its transporters deregulated? Additionally, can we truly expect to find a universal model to explain this selectivity? In this report, we aim to provide our peers with an overview of the currently known mitochondrial heme metabolism and approaches that could explain, at least partly, the mechanism of protoporphyrin IX selectivity towards cancer cells.
Emerging Roles of the Iron Chelators in Inflammation
Authors: Di Paola Alessandra , Tortora Chiara , Argenziano Maura , Marrapodi Maria Maddalena , Rossi Francesca
Published: July 2022

Iron is a crucial element for mammalian cells, considering its intervention in several physiologic processes. Its homeostasis is finely regulated, and its alteration could be responsible for the onset of several disorders. Iron is closely related to inflammation; indeed, during inflammation high levels of interleukin-6 cause an increased production of hepcidin which induces a degradation of ferroportin. Ferroportin degradation leads to decreased iron efflux that culminates in elevated intracellular iron concentration and consequently iron toxicity in cells and tissues. Therefore, iron chelation could be considered a novel and useful therapeutic strategy in order to counteract the inflammation in several autoimmune and inflammatory diseases. Several iron chelators are already known to have anti-inflammatory effects, among them deferiprone, deferoxamine, deferasirox, and Dp44mT are noteworthy. Recently, eltrombopag has been reported to have an important role in reducing inflammation, acting both directly by chelating iron, and indirectly by modulating iron efflux. This review offers an overview of the possible novel biological effects of the iron chelators in inflammation, suggesting them as novel anti-inflammatory molecules.
Characterization and Functional Implications of the Nonexpressor of Pathogenesis-Related Genes 1 (NPR1) in Saccharum
Authors: Zang Shoujian , Qin Liqian , Zhao Zhennan , Zhang Jing , Zou Wenhui , Wang Dongjiao , Feng Aoyin , Yang Shaolin , Que Youxiong , Su Yachun
Published: July 2022

Sugarcane (Saccharum spp.) is an important sugar and energy crop worldwide. As a core regulator of the salicylic acid (SA) signaling pathway, nonexpressor of pathogenesis-related genes 1 (NPR1) plays a significant role in the response of the plant to biotic and abiotic stresses. However, there is currently no report on the NPR1-like gene family in sugarcane. In this study, a total of 18 NPR1-like genes were identified in Saccharum spontaneum and classified into three clades (clade I, II, and III). The cis-elements predicted in the promotors revealed that the sugarcane NPR1-like genes may be involved in various phytohormones and stress responses. RNA sequencing and quantitative real-time PCR analysis demonstrated that NPR1-like genes were differentially expressed in sugarcane tissues and under Sporisorium scitamineum stress. In addition, a novel ShNPR1 gene from Saccharum spp. hybrid ROC22 was isolated by homologous cloning and validated to be a nuclear-localized clade II member. The ShNPR1 gene was constitutively expressed in all the sugarcane tissues, with the highest expression level in the leaf and the lowest in the bud. The expression level of ShNPR1 was decreased by the plant hormones salicylic acid (SA) and abscisic acid (ABA). Additionally, the transient expression showed that the ShNPR1 gene plays a positive role in Nicotiana benthamiana plants’ defense response to Ralstonia solanacearum and Fusarium solani var. coeruleum. This study provided comprehensive information for the NPR1-like family in sugarcane, which should be helpful for functional characterization of sugarcane NPR1-like genes in the future.
Assessing the Morphological and Behavioral Toxicity of Catechol Using Larval Zebrafish
Authors: Morash Michael G. , Soanes Kelly H. , Achenbach John C. , Ellis Lee D.
Published: July 2022

Catechol is a ubiquitous chemical used in the manufacturing of fragrances, pharmaceuticals and flavorants. Environmental exposure occurs in a variety of ways through industrial processes, during pyrolysis and in effluent, yet despite its prevalence, there is limited information regarding its toxicity. While the genotoxicity and gastric carcinogenicity of catechol have been described in depth, toxicological studies have potentially overlooked a number of other effects relevant to humans. Here, we have made use of a general and behavioral larval zebrafish toxicity assay to describe previously unknown catechol-based toxicological phenomena. Behavioral testing revealed catechol-induced hypoactivity at concentrations an order of magnitude lower than observable endpoints. Catechol exposure also resulted in punctate melanocytes with concomitant decreases in the expression of pigment production and regulation markers mitfa, mc1r and tyr. Because catechol is converted into a number of toxic metabolites by tyrosinase, an enzyme found almost exclusively in melanocytes, an evaluation of the effects of catechol on these cells is critical to evaluating the safety of this chemical. This work provides insights into the toxic nature of catechol and highlights the benefits of the zebrafish larval testing platform in being able to dissect multiple aspects of toxicity with one model.
A Computational–Experimental Investigation of the Molecular Mechanism of Interleukin-6-Piperine Interaction
Authors: Povinelli Ana Paula Ribeiro , Zazeri Gabriel , Jones Alan M. , Cornélio Marinônio Lopes
Published: July 2022

Herein, we elucidate the biophysical aspects of the interaction of an important protein, Interleukin-6 (IL6), which is involved in cytokine storm syndrome, with a natural product with anti-inflammatory activity, piperine. Despite the role of piperine in the inhibition of the transcriptional protein NF-κB pathway responsible for activation of IL6 gene expression, there are no studies to the best of our knowledge regarding the characterisation of the molecular interaction of the IL6-piperine complex. In this context, the characterisation was performed with spectroscopic experiments aided by molecular modelling. Fluorescence spectroscopy alongside van’t Hoff analyses showed that the complexation event is a spontaneous process driven by non-specific interactions. Circular dichroism aided by molecular dynamics revealed that piperine caused local α-helix reduction. Molecular docking and molecular dynamics disclosed the microenvironment of interaction as non-polar amino acid residues. Although piperine has three available hydrogen bond acceptors, only one hydrogen-bond was formed during our simulation experiments, reinforcing the major role of non-specific interactions that we observed experimentally. Root mean square deviation (RMSD) and hydrodynamic radii revealed that the IL6-piperine complex was stable during 800 ns of simulation. Taken together, these results can support ongoing IL6 drug discovery efforts.
Amyloid Properties of the FXR1 Protein Are Conserved in Evolution of Vertebrates
Authors: Velizhanina Maria E. , Galkin Alexey P.
Published: July 2022

Functional amyloids are fibrillary proteins with a cross-β structure that play a structural or regulatory role in pro- and eukaryotes. Previously, we have demonstrated that the RNA-binding FXR1 protein functions in an amyloid form in the rat brain. This RNA-binding protein plays an important role in the regulation of long-term memory, emotions, and cancer. Here, we evaluate the amyloid properties of FXR1 in organisms representing various classes of vertebrates. We show the colocalization of FXR1 with amyloid-specific dyes in the neurons of amphibians, reptiles, and birds. Moreover, FXR1, as with other amyloids, forms detergent-resistant insoluble aggregates in all studied animals. The FXR1 protein isolated by immunoprecipitation from the brains of different vertebrate species forms fibrils, which show yellow-green birefringence after staining with Congo red. Our data indicate that in the evolution of vertebrates, FXR1 acquired amyloid properties at least 365 million years ago. Based on the obtained data, we discuss the possible role of FXR1 amyloid fibrils in the regulation of vital processes in the brain of vertebrates.
Liposome Formulation and In Vitro Testing in Non-Physiological Conditions Addressed to Ex Vivo Kidney Perfusion
Authors: Pisani Silvia , Chiesa Enrica , Genta Ida , Dorati Rossella , Gregorini Marilena , Grignano Maria Antonietta , Ramus Marina , Ceccarelli Gabriele , Croce Stefania , Valsecchi Chiara , Monti Manuela , Rampino Teresa , Conti Bice
Published: July 2022

This work focuses on formulating liposomes to be used in isolated kidney dynamic machine perfusion in hypothermic conditions as drug delivery systems to improve preservation of transplantable organs. The need mainly arises from use of kidneys from marginal donors for transplantation that are more exposed to ischemic/reperfusion injury compared to those from standard donors. Two liposome preparation techniques, thin film hydration and microfluidic techniques, are explored for formulating liposomes loaded with two model proteins, myoglobin and bovine serum albumin. The protein-loaded liposomes are characterized for their size by DLS and morphology by TEM. Protein releases from the liposomes are tested in PERF-GEN perfusion fluid, 4 °C, and compared to the in vitro protein release in PBS, 37 °C. Fluorescent liposome uptake is analyzed by fluorescent microscope in vitro on epithelial tubular renal cell cultures and ex vivo on isolated pig kidney in hypothermic perfusion conditions. The results show that microfluidics are a superior technique for obtaining reproducible spherical liposomes with suitable size below 200 nm. Protein encapsulation efficiency is affected by its molecular weight and isoelectric point. Lowering incubation temperature slows down the proteins release; the perfusion fluid significantly affects the release of proteins sensitive to ionic media (such as BSA). Liposomes are taken up by epithelial tubular renal cells in two hours’ incubation time.
New Frontiers in Neurodegeneration and Regeneration Associated with Brain-Derived Neurotrophic Factor and the rs6265 Single Nucleotide Polymorphism
Authors: Szarowicz Carlye A. , Steece-Collier Kathy , Caulfield Margaret E.
Published: July 2022

Brain-derived neurotrophic factor is an extensively studied neurotrophin implicated in the pathology of multiple neurodegenerative and psychiatric disorders including, but not limited to, Parkinson’s disease, Alzheimer’s disease, Huntington’s disease, traumatic brain injury, major de-pressive disorder, and schizophrenia. Here we provide a brief summary of current knowledge on the role of BDNF and the common human single nucleotide polymorphism, rs6265, in driving the pathogenesis and rehabilitation in these disorders, as well as the status of BDNF-targeted therapies. A common trend has emerged correlating low BDNF levels, either detected within the central nervous system or peripherally, to disease states, suggesting that BDNF replacement therapies may hold clinical promise. In addition, we introduce evidence for a distinct role of the BDNF pro-peptide as a biologically active ligand and the need for continuing studies on its neurological function outside of that as a molecular chaperone. Finally, we highlight the latest research describing the role of rs6265 expression in mechanisms of neurodegeneration as well as paradoxical advances in the understanding of this genetic variant in neuroregeneration. All of this is discussed in the context of personalized medicine, acknowledging there is no “one size fits all” therapy for neurodegenerative or psychiatric disorders and that continued study of the multiple BDNF isoforms and genetic variants represents an avenue for discovery ripe with therapeutic potential.
Spectroscopic Analyses and Antimicrobial Activity of Novel Ciprofloxacin and 7-Hydroxy-4-methylcoumarin, the Plant-Based Natural Benzopyrone Derivative
Authors: El-Attar Mohamed S. , Sadeek Sadeek A. , Abd El-Hamid Sherif M. , Elshafie Hazem S.
Published: July 2022

Coumarin is highly distributed in nature, notably in higher plants. The biological features of coumarin include antibacterial, anticancer and antioxidant effects. It is well known that metal ions present in complexes accelerate the drug action and the efficacy of organic therapeutic agents. The main aim of the current study is the synthesis of different complexes of the interaction between ciprofloxacin hydrochloride (CIP) and coumarin derivative 7-hydroxy-4-methylcoumarin (HMC) with Zr(IV). The chelates of CIP with Zr(IV) were prepared and characterized by elemental analysis, melting point, conductance measurements, spectroscopic techniques involving IR, UV-Vis, 1H NMR, and thermal behavior (TG-DTG) in the presence of HMC, dimethylformamide (DMF), pyridine (Py), and triethylamine (Et3N). Results of molar conductivity tests showed that the new synthesized complexes are electrolytes with a 1:1 or 1:2 electrolyte ratio, with the chloride ions functioning as counter ions. According to IR spectra, CIP acts as a neutral bidentate ligand with Zr(IV) through one carboxylato oxygen and the carbonyl group, HMC as a monodentate through the carbonyl group, and DMF through the oxygen atom of the carbonyl group and the N atom of Py and Et3N. The thermal behavior of the complexes was carefully investigated using TG and DTG techniques. TG findings signal that water molecules are found as hydrated and coordinated. The thermal decomposition mechanisms proposed for CIP, HMC, and Zr(IV) complexes are discussed and the activation energies (Ea), Gibbs free energies (∆G*), entropies (∆S*), and enthalpies (∆H*) of thermal decomposition reactions have been calculated using Coats–Redfern (CR) and Horowitz–Metzeger (HM) methods. The studied complexes were tested against some human pathogens and phytopathogens, including three Gram-positive bacteria (Bacillus subtilis, B. cereus, Brevibacterium otitidis) and three Gram-negative bacteria (Escherichiacoli, Pseudomonas aeruginosa and Klebsiella pneumoniae), and compared to the free CIP and HMC parent compounds.
Drivers Influencing the Adoption Intention towards Mobile Fintech Services: A Study on the Emerging Bangladesh Market
Authors: Hassan Md. Sharif , Islam Md. Aminul , Sobhani Farid Ahammad , Nasir Hussen , Mahmud Imroz , Zahra Fatema Tuz
Published: July 2022

People’s acceptance of technological changes has escalated with time. However, the acceptance and adoption of fintech services hiked after the outbreak of the virulent coronavirus. With this breakout, the adoption of mobile fintech services (MFS) increased among general citizens and business sectors around the world, including in developed, emerging, and developing economies. This study aimed to identify the factors that impact the adoption intention of consumers to embrace and enhance the use of mobile fintech services in an emerging market, Bangladesh. A research model was developed to strengthen the objective of this paper. A total of 218 respondents responded to the questionnaire. The study utilized structural equation modeling to analyze the results in SmartPLS software. The results showed significant positive effects of social influence, trust, perceived benefit, and facilitating conditions on the adoption intention towards MFS. Mobile fintech service providers must keep their users’ needs and literacy rates in mind when designing the user interface (UI). Moreover, they should also cater more efficient services to the users and work based on the feedback received. The customers’ satisfaction will ultimately lead to customers conducting more digital transactions and will contribute to the escalation of fintech transactions, resulting in more financial inclusion.
Reconstruction as Deterioration Indicator for Operational Structural Performances of Bridge Materials
Authors: Farhey Daniel N.
Published: July 2022

This article presents analysis of deterioration indicators for a comparative quantification of the operational structural performances of bridge inventories. The analysis considers the various kinds of material in the entire database of the US National Bridge Inventory. The multi-criteria approach integrates the indicators of deterioration, considering diagnostic condition and life cycle to determine the equivalent operational structural performances. This study also uses reconstruction data to determine an additional deterioration indicator for performance. The proportional effort for reconstruction reflects the practical need to counter deterioration and preserve a required level of structural performance, under all circumstances. The presented addition of reconstruction performance provides a more comprehensive assessment of bridge performance. The results reveal the continuous efforts to maintain a relatively balanced performance adequacy at the national level within the prevailing funding constraints. Reconstruction performances are generally higher than deterioration trends (rate and pattern) performances, revealing that the constrained reconstruction efforts have difficulty to keep pace with the actual deterioration.
A New Species of Limnephilus (Insecta: Trichoptera: Limnephilidae) from China, with Revision of the Genus Limnephilus on the Chinese Mainland
Authors: Zang Haoming , Ge Xinyu , Peng Lang , Sun Changhai , Wang Beixin
Published: July 2022

Fifty individuals of Limnephilus from the Qinghai Province, China, were examined, and their COI barcode sequences were extracted and analyzed. Forty individuals of Limnephilus from the Insect Collection of Nanjing Agricultural University (ICNAU), China, were examined, and photos of the male genitalia of four Limnephilus species are here presented. The males, females, larvae, and pupae of a new species, Limnephilus deqianensis n. sp., associated via COI barcode sequences, are described and illustrated. Ecological photos of the male, pupal case, and the habitat of the new species L. deqianensis n. sp. are also provided. Five species groups containing all seventeen Chinese Limnephilus species are revised. Diagnoses, keys, and a distribution map of them are provided. All of the sequences have been uploaded to GenBank. All specimens are deposited in the ICNAU, Nanjing, Jiangsu Province, P. R. China.
A New Cretaceous Dustywing Genus (Neuroptera: Coniopterygidae) with Peculiar Wing Venation
Authors: Chen Zuluan , Wang Lihua , Zhuo De , Xu Chunpeng , Liu Xingyue
Published: July 2022

The species and morphological diversity of dustywings (Neuroptera: Coniopterygidae) from the Cretaceous, of which the knowledge is rapidly increasing by recent studies on the species from the mid-Cretaceous Kachin amber, provide valuable evidence for understanding the phylogeny and early evolution of this highly specialized lacewing lineage. Here we describe a new genus and two new species of this genus in Coniopterygidae from the mid-Cretaceous (lowermost Cenomanian) of northern Myanmar, namely Paradoxoconis szirakii gen. et sp. nov. and Paradoxoconis longipalpa gen. et sp. nov. The new genus possesses a peculiar combination of wing characters, e.g., the terminal fusion or connection between ScP and RA, the terminal connection of RA to RP, the presence of forewing A3, and the presence of a distal gradate series of crossveins. Despite uncertain subfamilial placement, this new genus morphologically resembles the extant genus Coniocompsa Enderlein, 1905 of the subfamily Aleuropteryginae and the extant genus Flintoconis Sziráki, 2007 of the subfamily Brucheiserinae. Our finding highlights the palaeodiversity of dustywings from the Cretaceous.
Effects of Metformin on Bone Mineral Density and Adiposity-Associated Pathways in Animal Models with Type 2 Diabetes Mellitus: A Systematic Review
Authors: Loh Darren Kin Wai , Kadirvelu Amudha , Pamidi Narendra
Published: July 2022

Recently, there have been investigations on metformin (Met) as a potential treatment for bone diseases such as osteoporosis, as researchers have outlined that type 2 diabetes mellitus (T2DM) poses an increased risk of fractures. Hence, this systematic review was conducted according to the 2020 PRISMA guidelines to evaluate the evidence that supports the bone-protective effects of metformin on male animal models with T2DM. Five databases—Google Scholar, PubMed, Wiley Online Library, SCOPUS, and ScienceDirect—were used to search for original randomized controlled trials published in English with relevant keywords. The search identified 18 articles that matched the inclusion criteria and illustrated the effects of Met on bone. This study demonstrates that Met improved bone density and reduced the effects of T2DM on adiposity formation in the animal models. Further research is needed to pinpoint the optimal dosage of Met required to exhibit these therapeutic effects.
New Insights into Immunotherapy for Gynecological Cancer
Authors: Kobori Takuro
Published: July 2022

Gynecologic malignancies are a heterogeneous group of female reproductive system tumors, including cervical, endometrial, ovarian, vaginal, and vulval cancers, and are the second most commonly diagnosed female cancers around the world [...]
Delayed Gastric Emptying Does Not Influence Cancer-Specific Survival after Pancreatoduodenectomy for Pancreatic Ductal Adenocarcinoma
Authors: Pillny Christiane , Teschke Jessica , Enderes Jana , Manekeller Steffen , Kalff Jörg C. , Glowka Tim R.
Published: July 2022

Background: Delayed gastric emptying (DGE) remains the most frequent complication following pancreatoduodenectomy (PD). The present study investigates the influence of delayed gastric emptying on cancer-specific survival after PD. Methods: We included 267 patients who underwent PD between 2014 and 2021. They were analyzed regarding demographic factors, pre- and perioperative characteristics, surgical complications, and long-term survival. Results: Patients with a higher Charlson Comorbidity Index (CCI) or pre-existing pulmonary disease suffered significantly more from DGE. When experiencing PPH, a prolonged hospital stay, or major overall complications (Clavien-Dindo °III-V) were more common in the DGE group. Tumor size over 3 cm negatively affected survival. Conclusions: DGE has no influence on long-term survival in PDAC patients, although it prolongs hospital stay.
Applications of Robotic Surgery in Thoracic Diseases
Authors: Casiraghi Monica , Mariolo Alessio Vincenzo , Spaggiari Lorenzo
Published: July 2022

With the ever-expanding implement of screening programs, as well as a raised awareness of patients about their own health, the number of cases of early-stage lung cancer is progressively increasing, leading surgeons to adapt their practice and to develop new surgical techniques that are less and less invasive [...]
Non-Renal Risk Factors for Chronic Kidney Disease in Liver Recipients with Functionally Intact Kidneys at 1 Month
Authors: Kim Deok-Gie , Hwang Shin , Kim Jong Man , Ryu Je Ho , You Young Kyoung , Choi Donglak , Kim Bong-Wan , Kim Dong-Sik , Nah Yang Won , Kim Tae-Seok , Cho Jai Young , Hong Geun , Yang Jae Do , Han Jaryung , Suh Suk-Won , Kim Kwan Woo , Jung Yun Kyung , Moon Ju Ik , Lee Jun Young , Kim Sung Hwa , Lee Jae Geun , Kim Myoung Soo , Lee Kwang-Woong , Joo Dong Jin
Published: July 2022

Chronic kidney disease (CKD) is a critical complication of liver transplants, of which non-renal risk factors are not fully understood yet. This study aimed to reveal pre- and post-transplant risk factors for CKD (<60 mL/min/1.73 m2), examining liver recipients with functionally intact kidneys one month after grafting using nationwide cohort data. Baseline risk factors were analyzed with multivariable Cox regression analyses and post-transplant risk factors were investigated with the time-dependent Cox model and matched analyses of time-conditional propensity scores. Of the 2274 recipients with a one-month eGFR ≥ 60 mL/min/1.73 m2, 494 (22.3%) developed CKD during a mean follow-up of 36.6 ± 14.4 months. Age, female sex, lower body mass index, pre-transplant diabetes mellitus, and lower performance status emerged as baseline risk factors for CKD. Time-dependent Cox analyses revealed that recurrent hepatocellular carcinoma (HR = 1.93, 95% CI 1.06–3.53) and infection (HR = 1.44, 95% CI 1.12–1.60) were significant post-transplant risk factors for CKD. Patients who experienced one of those factors showed a significantly higher risk of subsequent CKD compared with the matched controls who lacked these features (p = 0.013 for recurrent hepatocellular carcinoma, and p = 0.003 for infection, respectively). This study clarifies pre- and post-transplant non-renal risk factors, which lead to renal impairment after LT independently from patients’ renal functional reserve.
The Eyelid Angiosarcoma: A Systematic Review of Characteristics and Clinical Course
Authors: Hwang Gyudeok , Shin Jeongah , Lee Ji-Young , Na Kyung-Sun , Paik Ji-Sun , Yim Hyeon Woo , Yang Suk-Woo , Cho Won-Kyung
Published: July 2022

A systematic search for eyelid angiosarcoma was performed from inception to December 2020 in Medline, EMBASE, and the Cochrane databases. Forty two eyelid angiosarcoma cases in 32 articles were analyzed. Eyelid angiosarcomas showed an incidence peak in the eighth decade of life, and was reported more frequently in Caucasian males. Eyelid angiosarcomas were associated with a mortality rate of 26.2%, a recurrence rate of 14.3%, and a cure rate of 45.2%. Four years event-free survival (EFS) rate was 36.0%, with median EFS of 36 months. Eyelid angiosarcomas with bilateral involvement or metastasis showed higher mortality and recurrence rates than unilateral eyelid invasion cases. In the prognosis analysis according to treatment modalities, the mortality and recurrence rates were the lowest in patients who underwent surgical excision. The 4-year EFS probability in a group with surgical excision was 60.6%, but in a group without surgical excision it was 30.3%. A total of 45.2% of the cases was misdiagnosed and 21.4% of the cases could not be correctly diagnosed with the first biopsy trial. The prognosis for eyelid angiosarcomas was better than that of angiosarcomas invading the face and scalp. Surgical excision was the most important treatment modality; thus, should be considered as the first treatment of choice.
Serum Concentrations of Ischaemia-Modified Albumin in Acute Coronary Syndrome: A Systematic Review and Meta-Analysis
Authors: Mangoni Arduino A. , Zinellu Angelo
Published: July 2022

The identification of novel circulating biomarkers of acute coronary syndrome (ACS) may improve diagnosis and management. We conducted a systematic review and meta-analysis of ischaemia-modified albumin (IMA), an emerging biomarker of ischaemia and oxidative stress, in ACS. We searched PubMed, Web of Science, and Scopus from inception to March 2022, and assessed the risk of bias and certainty of evidence with the Joanna Briggs Institute Critical Appraisal Checklist and GRADE, respectively. In 18 studies (1654 ACS patients and 1023 healthy controls), IMA concentrations were significantly higher in ACS (standard mean difference, SMD = 2.38, 95% CI 1.88 to 2.88; p < 0.001; low certainty of evidence). The effect size was not associated with pre-defined study or patient characteristics, barring the country where the study was conducted. There were no significant differences in effect size between acute myocardial infarction (MI) and unstable angina (UA), and between ST-elevation (STEMI) and non-ST-elevation MI (NSTEMI). However, the effect size was progressively larger in UA (SMD = 1.63), NSTEMI (SMD = 1.91), and STEMI (3.26). Our meta-analysis suggests that IMA might be useful to diagnose ACS. Further studies are warranted to compare the diagnostic performance of IMA vs. established markers, e.g., troponin, and to determine its potential utility in discriminating between UA, NSTEMI, and STEMI (PROSPERO registration number: CRD42021324603).
Trunk Alignment in Physically Active Young Males with Low Back Pain
Authors: Plandowska Magdalena , Kędra Agnieszka , Kędra Przemysław , Czaprowski Dariusz
Published: July 2022

Background: Systematic physical activity has become an essential part of the guidelines for the prevention and treatment of low back pain (LBP). The aim of this study was to assess differences in trunk alignment parameters with regard to the level of physical activity in groups of individuals with and without LBP. Methods: 43 participants with LBP and 37 healthy persons were recruited. Participants were divided into two subgroups: (1) students with a moderate level of physical activity (MPA); (2) students with a high level of physical activity (HPA). An original questionnaire was used to assess the prevalence of LBP. The spinal posture was measured using the Formetric 4D rasterstereographic system. Results: There were no significant differences between groups for any of the parameters assessed: trunk imbalance, trunk inclination, trunk torsion, pelvic tilt, pelvic inclination, pelvic torsion, kyphotic angle and lordotic angle. Conclusions: There are no differences in trunk alignment parameters in the sagittal, frontal and transversal planes between physically active males with and without LBP. Therefore, it can be assumed that physical activity may reduce the risk of the deterioration of trunk alignment in males with LBP younger than 25 years.
Primary and Secondary Mitochondrial Diseases: Etiologies and Therapeutic Strategies
Authors: Valenti Daniela , Vacca Rosa
Published: July 2022

Mitochondria are complex and multifaceted organelles that constitute a dynamic network of signaling platforms playing a pivotal role in cellular energy-generating processes [...]
Pleomorphic Adenoma of the Salivary Glands and Epithelial–Mesenchymal Transition
Authors: Matsumiya-Matsumoto Yuka , Morita Yoshihiro , Uzawa Narikazu
Published: July 2022

Pleomorphic adenoma (PA) is a localized tumor that presents pleomorphic or mixed characteristics of epithelial origin and is interwoven with mucoid tissue, myxoid tissue, and chondroid masses. The literature reported that PA most often occurs in adults aged 30–60 years and is a female predilection; the exact etiology remains unclear. Epithelial–mesenchymal transition (EMT) is the transdifferentiation of stationary epithelial cells primarily activated by a core set of transcription factors (EMT-TFs) involved in DNA repair and offers advantages under various stress conditions. Data have suggested that EMTs represent the basic principle of tissue heterogeneity in PAs, demonstrating the potential of adult epithelial cells to transdifferentiate into mesenchymal cells. It has also been reported that multiple TFs, such as TWIST and SLUG, are involved in EMT in PA and that SLUG could play an essential role in the transition from myoepithelial to mesenchymal cells. Given this background, this review aims to summarize and clarify the involvement of EMT in the development of PA, chondrocyte differentiation, and malignant transformation to contribute to the fundamental elucidation of the mechanisms underlying EMT.
The Quality of Life in Citizens with Oropharyngeal Dysphagia—A Cross-Sectional Study
Authors: Bendsen Bettina Burgdorff , Jensen Diana , Westmark Signe , Krarup Anne Lund , Riis Johannes , Melgaard Dorte
Published: July 2022

Dysphagia is a risk factor that impaires an individual’s experience of mealtimes. Few studies contribute to the knowledge on the health-related quality of life (HRQoL) of citizens with oropharyngeal dysphagia (OD) living independently. The aim of this study involves evaluating the HRQoL in citizens living independently and suffering from OD. This cross-sectional study was performed in seven municipalities in Denmark between March 2019 and December 2020. The 90 citizens included (54% female, mean age 76.6 years (SD 0.8)) were ≥18 years, as well as diagnosed with OD using the volume-viscosity swallow test and Minimal Eating Observation Form version II. The Dysphagia Handicap Index-DK, Barthel 20, and European Quality of Life-5 Dimensions were fulfilled. Of the participating citizens, 66% of them needed additional time to eat, 64% coughed while eating, and 58% coughed while drinking. Additionally, 60% reported having a dry mouth, 62% needed to drink to succeed with swallowing foods, and 57% had to swallow multiple times. About one-third felt embarrassed when eating with others. They could not enjoy eating in the same manner as they had previously, and/or felt handicapped or limited. OD was shown to have had a high impact on the QoL in citizens with OD living independently. Focus is needed on xerostomia, as well as on the psychological aspects pertainings to mealtimes for citizens with OD.
Cross-Cultural Adaptation and Psychometric Properties of the French Version of the EXIT to Measure Women’s Experiences of Induction of Labor
Authors: Guiguet-Auclair Candy , Rouzaire Marion , Debost-Legrand Anne , Dissard Sigrid , Rouille Manon , Delabaere Amélie , Gallot Denis
Published: July 2022

Background: In France, more than 20% of women require induction of labor (IOL), which can be psychologically and emotionally challenging for patients. It is important to assess how they feel about their IOL experiences. Our aim was to cross-culturally adapt and evaluate the psychometric properties of a French version of the EXIT to assess women’s experiences of IOL. Methods: The EXIT was cross-culturally adapted by conducting forward and backward translations following international guidelines. A cross-sectional study was conducted to assess the psychometric properties of the ten French EXIT items: data completeness, factor analysis, internal consistency, score distribution, floor and ceiling effects, inter-subscale correlations, convergent validity, and test–retest reliability. Results: The EXIT was successfully cross-culturally adapted to the French context and any IOL method. The results obtained from 163 patients requiring IOL showed good acceptability. Exploratory factor analysis resulted in a three-factor solution with subscales reflecting the experiential aspects of time taken to give birth, discomfort with IOL, and subsequent contractions. Good internal consistency (Cronbach’s alpha or Spearman correlation coefficients ranging from 0.55 to 0.84) and good test–retest reliability (intraclass correlation coefficients ranging from 0.66 to 0.85) for the three identified subscales were found. Conclusions: The ten-item French EXIT is a valid and reliable instrument for the self-assessment of women’s experiences of IOL in the three weeks following delivery for any method of IOL used. As a patient-reported outcome measure, it would allow the comparison of experiential outcomes across IOL studies in order to include women’s preferences in decisions regarding their care.
Pupillometry as a Measure of Listening Effort in Patients with Bone-Anchored Hearing Systems
Authors: Gawęcki Wojciech , Krzystanek Katarzyna , Węgrzyniak Magdalena , Gibasiewicz Renata , Wierzbicka Małgorzata
Published: July 2022

The goal of this study is to assess speech comprehension and listening effort by means of pupillometry, in patients with bone-anchored hearing system (BAHS). The study was performed prospectively in a group of 21 hearing-impaired adults, unilaterally implanted with BAHS Ponto. Listening effort was compared in patients wearing two sound processors (Oticon Medical AB, Askim, Sweden): Ponto 3 SuperPower (P3SP) and Ponto Pro (PP). Every patient was invited to two visits, separated by a 3-month break. The first session was to establish the noise level needed to obtain 95% correct sentence recall in the hearing in noise test (HINT), when speech is presented at 70 dB SPL. During the second session, pupillometry, with the use of the above-mentioned conditions, was performed. The mean HINT scores obtained during the second visit were 96.3% for PP and 97.7% for P3SP (p = 0.9863). In pupillometry, no significant differences were found for average PPD (peak pupil dilation; p = 0.3247), average peak pupil dilation timing (p = 0.527) and for pupil dilation growth curves with both processors. The findings of this study suggest that BAHS users allocate similar listening effort with PP and P3SP when processing speech-in-noise at a sound pressure level not saturating Ponto Pro and at a fixed performance level of 95%. This finding applies to the patients who meet the qualification criteria for bone conduction devices and have BC in situ threshold average below 45 dB HL.
Differential Regulation of CD45 Expression on Granulocytes, Lymphocytes, and Monocytes in COVID-19
Authors: Ahmed Muhammad G. T. , Limmer Andreas , Sucker Christoph , Fares Khaled Mohamed , Mohamed Sahar Abdel-Baky , Othman Ahmed H. , Berger Marc Moritz , Brenner Thorsten , Hartmann Matthias
Published: July 2022

CD45 is a transmembrane glycoprotein and protein tyrosine phosphatase expressed on the surface of all nucleated hematopoietic cells. While there is increasing evidence demonstrating the involvement of CD45 in immune system regulation, no information on CD45 expression in inflammation and sepsis is currently available. Therefore, we determined the CD45 surface expression on granulocytes, lymphocytes, and monocytes in patients with COVID-19 and healthy volunteers in both absence and presence of lipopolysaccharide (LPS). Following approval by the local ethics committee, whole blood samples were obtained from patients with COVID-19 infection on day 1 of hospital admission and healthy volunteers. Samples were incubated in absence and presence of LPS and CD45 was measured in granulocytes, lymphocytes, and monocytes using flow cytometry. In comparison with healthy individuals, COVID-19 patients showed an increased CD45 expression on the surface of granulocytes (+35%, p < 0.02) and lymphocytes (+39%, p < 0.0001), but a reduced CD45 expression on monocytes (−35%, p < 0.0001). LPS incubation of whole blood from healthy individuals increased the CD45 expression on granulocytes (+430%, p < 0.0001), lymphocytes (+32%, p = 0.0012), and monocytes (+36%, p = 0.0005), respectively. LPS incubation of whole blood samples from COVID-19 patients increased the CD45 expression on granulocytes and monocytes, and decreased the CD45 expression on lymphocytes. In conclusion, CD45 expression on leucocytes is altered: (1) in COVID-19 patients, and (2) in in vitro endotoxemia in a complex cell-specific way, thus representing a new immunoregulatory mechanism.
Dissolution Behavior of Hydrothermally Treated Hydroxyapatite–Titanium Nitride Films Coated on PEEK: In Vitro Study
Authors: Boonpok Siriwat , Koonrungsrisomboon Kwanchanok , Suttiat Kullapop , Yavirach Piriya , Boonyawan Dhreerawan
Published: July 2022

Polyetheretherketone (PEEK) has become an alternative material for orthopaedics and dental implants. However, bio-inertness is an important limitation in this material. In the present study, a hydroxyapatite (HA)–titanium nitride (TiN) coating was fabricated via pulsed DC magnetron sputtering and treated with hydrothermal treatment to improve the bioactive property of PEEK. The dissolution behavior of the coating was studied in simulated body fluid solution (SBF) at 1, 3, 5, 7, 14, 21, 28, and 56 days. The coating surface was analyzed before and after the immersion process by X-ray photoelectron spectroscopy (XPS), atomic force microscope (AFM), and scanning electron microscope (SEM). The calcium and phosphorus concentration alteration in SBF was quantified by an inductively coupled plasma-optical emission spectrometer (ICP-OES). Coating dissolution and the precipitation of calcium phosphate complex from SBF were observed as occurring suddenly and continuously throughout the immersion times. These processes resulted in an alteration in both physical and chemical coating properties. After 56 days, the coating remained on PEEK surfaces and the Ca/P ratio was 1.16. These results indicate that HA-TiN coating via pulsed DC magnetron sputtering followed by hydrothermal treatment improved the bioactivity of materials and provided a potential benefit to orthopedics and dental applications.
Hydroxyapatite Nanoparticles for Improved Cancer Theranostics
Authors: Kargozar Saeid , Mollazadeh Sahar , Kermani Farzad , Webster Thomas J. , Nazarnezhad Simin , Hamzehlou Sepideh , Baino Francesco
Published: July 2022

Beyond their well-known applications in bone tissue engineering, hydroxyapatite nanoparticles (HAp NPs) have also been showing great promise for improved cancer therapy. The chemical structure of HAp NPs offers excellent possibilities for loading and delivering a broad range of anticancer drugs in a sustained, prolonged, and targeted manner and thus eliciting lower complications than conventional chemotherapeutic strategies. The incorporation of specific therapeutic elements into the basic composition of HAp NPs is another approach, alone or synergistically with drug release, to provide advanced anticancer effects such as the capability to inhibit the growth and metastasis of cancer cells through activating specific cell signaling pathways. HAp NPs can be easily converted to smart anticancer agents by applying different surface modification treatments to facilitate the targeting and killing of cancer cells without significant adverse effects on normal healthy cells. The applications in cancer diagnosis for magnetic and nuclear in vivo imaging are also promising as the detection of solid tumor cells is now achievable by utilizing superparamagnetic HAp NPs. The ongoing research emphasizes the use of HAp NPs in fabricating three-dimensional scaffolds for the treatment of cancerous tissues or organs, promoting the regeneration of healthy tissue after cancer detection and removal. This review provides a summary of HAp NP applications in cancer theranostics, highlighting the current limitations and the challenges ahead for this field to open new avenues for research.
Hydraulic Performance of Seawater Intake System Using CFD Modeling
Authors: Yamini Omid Aminoroayaie , Movahedi Azin , Mousavi S. Hooman , Kavianpour Mohammad Reza , Kyriakopoulos Grigorios L.
Published: July 2022

In recent years, tapping the sea for potable water has gained prominence as a potential source of water. Since seawater intake systems are often used in the infrastructure industry, ensuring proper efficiency in different operating conditions is very important. In this paper, CFD modeling is used to show general hydraulic design (flow patterns, stream flow, vortex severities, and pre-swirl) principles and performance acceptability criteria for pump intakes in different conditions. The authors explore scenarios for avoiding or resolving hydraulic problems that have arisen as a result of hydraulic model studies. The results show that the designer should make every effort to avoid small entrance and filtration areas from the basin to the intake forebay bottom, which could result in jet outlet and/or supercritical flow; too small logs at the basin outflow, which could result in high velocity flow jets; and sudden area contractions at the forebay to pump bay junction. There should be enough submergence at the pumps to reduce harmful vortex severities and pre-swirl. Curtain walls, baffles, fillets, and splitters, as well as flow redistributors, can all aid in improving approach flow patterns. Reduced flow separations and eddies will be greatly assisted by rounding corners and providing guide walls. Using a numerical model to figure out what is wrong and how to fix it will help the facility’s costs and maintenance decrease in the long run.
Contourite and Turbidite Features in the Middle Caspian Sea and Their Connection to Geohazards Derived from High-Resolution Seismic Data
Authors: Yutsis Vsevolod , Levchenko Oleg , Putans Victoria
Published: July 2022

High fluvial input combined with specific topographic and oceanographic settings in the Caspian Sea create favorable conditions for contourite deposition. For the first time in its middle portion, contourite deposits have been observed in high-resolution seismic profiles. Various types of contourite drifts and mixed depositional systems have been revealed on the lower slope and in the adjacent basin, some of which are accompanied by sediment wave fields. The deposition of contourites or turbidites and their lateral distribution is controlled by sea-floor topography and oceanographic processes, as well as the modern activity of gravity flows downslope on the western Caucasian slope and in the channel system on the Mangyshlak Sill. The contourite drifts and sediment wave fields form several contourite depositional systems, which seem to merge in the Caspian contourite depositional complex. This occurs near the foot of slopes of the Derbent Basin and is related to the counterclockwise circum-Caspian current in the Middle Caspian Sea. The fact that the Caspian Sea is the largest lake in the world makes this region a significant area for research into the “lake contourites” issue. The Caspian Sea is an important oil-producing area, and sedimentary processes related to the contourite and turbidite can be a source of potential geohazards in the construction and exploitation of underwater engineering structures
Interval-Valued Fuzzy Multi-Criteria Decision-Making with Dependent Evaluation Criteria for Evaluating Service Performance of International Container Ports
Authors: Wang Yu-Jie , Liu Li-Jen , Han Tzeu-Chen
Published: July 2022

Due to COVID-19 barriers, the needs of international container ports have become more important than in the past. Therefore, it is very critical and essential for the scientific developments of port-logistics. To gain the scientific developments of port logistics, effective and efficient evaluation methods for decision-making are indispensable, especially for assessing service performance of international container ports based on dependent evaluation criteria (DEC). Among numerous decision-making methods, technique for order preference by similarity to ideal solution (TOPSIS) was often expanded under fuzzy environments into fuzzy multi-criteria decision-making (FMCDM) to preserve imprecise messages. The FMCDM was able to be associated with quality function deployment (QFD) into a hybrid method to solve problems with DEC. To gain more messages, QFD and TOPSIS are combined and then expanded under interval-valued fuzzy environment (IVFE) to solve a FMCDM problem with DEC. Practically, evaluating service performance of international container ports in Taiwan and the surrounding sea areas is considered a problem with DEC because the related evaluation criteria are partially connected. By the hybrid method of combining QFD with TOPSIS under IVFE, international container ports with DEC are effectively and efficiently evaluated for service performance, and more insights are gained than the past for establishing essential fundamentals in recent scientific developments of port logistics on account of breaking down COVID-19 barriers.