bluuebunny/crossref_metadata_2025 huggingface dataset:
Myopia progression after cessation of atropine in children: a systematic review and meta-analysis
Authors: Lee Ssu-Hsien , Tsai Ping-Chiao , Chiu Yu-Chieh , Wang Jen-Hung , Chiu Cheng-Jen
Published: January 2024

Purpose: To comprehensively assess rebound effects by comparing myopia progression during atropine treatment and after discontinuation.

Methods: A systematic search of PubMed, EMBASE, Cochrane CENTRAL, and ClinicalTrials.gov was conducted up to 20 September 2023, using the keywords “myopia," “rebound,” and “discontinue." Language restrictions were not applied, and reference lists were scrutinized for relevant studies. Our study selection criteria focused on randomized control trials and interventional studies involving children with myopia, specifically those treated with atropine or combination therapies for a minimum of 6 months, followed by a cessation period of at least 1 month. The analysis centered on reporting annual rates of myopia progression, considering changes in spherical equivalent (SE) or axial length (AL). Data extraction was performed by three independent reviewers, and heterogeneity was assessed using I2 statistics. A random-effects model was applied, and effect sizes were determined through weighted mean differences with 95% confidence intervals Our primary outcome was the evaluation of rebound effects on spherical equivalent or axial length. Subgroup analyses were conducted based on cessation and treatment durations, dosage levels, age, and baseline SE to provide a nuanced understanding of the data.

Results: The analysis included 13 studies involving 2060 children. Rebound effects on SE were significantly higher at 6 months (WMD, 0.926 D/y; 95%CI, 0.288–1.563 D/y; p = .004) compared to 12 months (WMD, 0.268 D/y; 95%CI, 0.077–0.460 D/y; p = .006) after discontinuation of atropine. AL showed similar trends, with higher rebound effects at 6 months (WMD, 0.328 mm/y; 95%CI, 0.165–0.492 mm/y; p < .001) compared to 12 months (WMD, 0.121 mm/y; 95%CI, 0.02–0.217 mm/y; p = .014). Sensitivity analyses confirmed consistent results. Shorter treatment durations, younger age, and higher baseline SE levels were associated with more pronounced rebound effects. Transitioning or stepwise cessation still caused rebound effects but combining optical therapy with atropine seemed to prevent the rebound effects.

Conclusion: Our meta-analysis highlights the temporal and dose-dependent rebound effects after discontinuing atropine. Individuals with shorter treatment durations, younger age, and higher baseline SE tend to experience more significant rebound effects. Further research on the rebound effect is warranted.

Systematic Review Registration: [https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=463093], identifier [registration number]
Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis
Authors: Wei Chengjian , Liang Jinchang , Wang Rui , Chi Luping , Wang Wenjing , Tan Jun , Shi Heli , Song Xueru , Cui Zhenzhen , Xie Qiang , Cheng Dejie , Wang Xiaoqiang
Published: January 2024

The soil microbial community plays a critical role in promoting robust plant growth and serves as an effective defence mechanism against root pathogens. Current research has focused on unravelling the compositions and functions of diverse microbial taxa in plant rhizospheres invaded by Ralstonia solanacearum, however, the specific mechanisms by which key microbial groups with distinct functions exert their effects remain unclear. In this study, we employed a combination of amplicon sequencing and metabolomics analysis to investigate the principal metabolic mechanisms of key microbial taxa in plant rhizosphere soil. Compared to the healthy tobacco rhizosphere samples, the bacterial diversity and co-occurrence network of the diseased tobacco rhizosphere soil were significantly reduced. Notably, certain genera, including Gaiella, Rhodoplanes, and MND1 (Nitrosomonadaceae), were found to be significantly more abundant in the rhizosphere of healthy plants than in that of diseased plants. Eight environmental factors, including exchangeable magnesium, available phosphorus, and pH, were found to be crucial factors influencing the composition of the microbial community. Ralstonia displayed negative correlations with pH, exchangeable magnesium, and cation exchange flux, but showed a positive correlation with available iron. Furthermore, metabolomic analysis revealed that the metabolic pathways related to the synthesis of various antibacterial compounds were significantly enriched in the healthy group. The correlation analysis results indicate that the bacterial genera Polycyclovorans, Lysobacter, Pseudomonas, and Nitrosospira may participate in the synthesis of antibacterial compounds. Collectively, our findings contribute to a more in-depth understanding of disease resistance mechanisms within healthy microbial communities and provide a theoretical foundation for the development of targeted strategies using beneficial microorganisms to suppress disease occurrence.
A qualitative study on the social representations of populism and democracy in Peru
Authors: Espinosa Agustin , Janos Erika , Pacheco Manuel , Cisneros Renzo , Cueva Fiorella , De Las Casas De La Lama Renato , De La Cruz Bellido Chrystian Noe , Grados Camila , Huapaya Carla , Mercado Sebastián , Palacios Danika , Reyes Jackeline Elena , Salazar Eleonora , Silva Mikel , Villa Kelly Patricia , Vera Angela
Published: January 2024

The purpose of this paper is to describe and analyze the social representations of democracy, populism and the relationship between both concepts in a sample of citizens from different regions of Peru (n = 76). To this end, a qualitative research design was proposed, using in-depth interviews, which were analyzed from a discursive approach. The results show that democracy and populism are two closely related concepts in tension. On the one hand, the social representation of democracy is semantically poor, closely related to electoral behavior and is seen as a political system that, ideally, is positively valued. Populism, on the other hand, is seen as a political strategy based on the manipulation of citizens' needs in order to reach power using the democratic process of elections. The representation of populism in general is negative, and it is mentioned that it appears and acquires strength in the face of citizen dissatisfaction with democracy, when it cannot solve problems of poverty, corruption or exclusion. The representations of populism take up the constitutive components proposed by different authors on the topic (people, elites and democracy as a product of popular will), but the participants do not necessarily structure the relationships between these components as proposed in the academic literature. Finally, the results shows that respondents' experiences with democracy and populism in Peru act as important socializing forces that will frame how citizens relate to politics and the public.
COVID-19 prevention and control measures and experiences during the 14th National Games of China: a qualitative interview study
Authors: Li Nan , Hou Shike , Zhang Yongzhong , Yao Lulu , Li Tiantian
Published: January 2024

Background

The 14th National Games was the first sporting mass gathering to be held in China in the context of the COVID-19 pandemic. It may increase the risk of severe acute respiratory syndrome coronavirus 2 transmission. In order to reduce the spread of the virus during the 14th National Games, the Chinese government took a series of public health measures, and ultimately no confirmed cases were found in the 14th National Games venues.

Objective

This study aimed to discuss preventive and control measures used to respond to the COVID-19 pandemic during the 14th National Games.

Methods

Five experts were selected for this study using a snowball sampling method, and semistructured and in-depth interviews were conducted. Based on grounded theory, the transcriptions were analysed and coded using Nvivo 12 software.

Results

A theoretical model of the COVID-19 prevention and control measures at the 14th National Games of China was constructed. The model contains seven main components: the health risks of mass gatherings, crowd management, emergency medical care, allocation of emergency medical resources, pandemic emergency drills, the pandemic prevention and control management platform (Quanyuntong app), and emergency response plans.

Conclusion

The study showed that the deployment of emergency medical resources was the most important for mass gatherings. This study not only expanded the applications of grounded theory but also serves as a reference for future scholars when conducting more in-depth empirical studies on public health countermeasures for mass gatherings and can inform organizers when holding mass gatherings.
Association between oxidative balance score and prostate specific antigen among older US adults
Authors: Li Jintao , Yang Chao , Xiang Kui
Published: January 2024

Objective

Oxidative Balance Score (OBS) is an index affecting the oxidative stress of dietary and lifestyle factors. We aimed to explore the association of OBS with prostate specific antigen (PSA) among older males.

Methods

A total of 5,136 samples were collected in this study to investigate the relationship between OBS and PSA from the National Health and Nutrition Examination Survey. Logistic regression models and restricted cubic spline were used to assess the associations between OBS and PSA.

Results

Compared with the Q1 group, the odds ratios for the association between OBS and PSA were 1.005 (1.003, 1.009), 1.003 (1.001, 1.006), and 1.001 (0.978, 1.022) for Q2, Q3, and Q4, respectively. In the age-specific analyses, the association was significant among individuals aged 65 years old and over: the odds ratios for the association between OBS and PSA were 1.019 (1.005, 1.028), 1.028 (1.018, 1.039), and 1.038 (1.022, 1.049) for Q2, Q3, and Q4, respectively. But it was not significant among individuals aged less than 65 years old: the odds ratios for the association between OBS and PSA were 1.016 (0.995, 1.026), 1.015 (0.985, 1.022), and 0.988 (0.978, 1.016) for Q2, Q3, and Q4, respectively. The restricted cubic splines also indicated a nonlinear relationship between OBS and PSA among individuals aged 65 years old and over (Poverall = 0.006, Pnonlinear = 0.021).

Conclusion

Our findings provide evidence that OBS is positively associated with higher levels of PSA among older adults. Further large-scale prospective cohort studies are needed to verify our findings.
Awareness and utilization of pre-exposure prophylaxis and HIV prevention services among transgender and non-binary adolescent and young adults
Authors: Rodriguez Arianna , Horvath Keith J. , Dowshen Nadia , Voss Raina , Warus Jonathan , Jacobs Megan , Kidd Kacie M. , Inwards-Breland David J. , Blumenthal Jill
Published: January 2024

Introduction

Transgender and gender non-binary (TGNB) individuals are disproportionally affected by HIV and face high rates of discrimination and stigmatization, resulting in limited access to HIV prevention services. Pre-exposure prophylaxis (PrEP) is highly efficacious for reducing the risk of HIV transmission. However, little research is available regarding PrEP awareness and utilization among TGNB adolescents and young adults (AYA).

Methods

TGNB AYA ages 15–24 years old were recruited between December 2021 and November 2022 for participation in a one-time, anonymous online survey study to assess PrEP awareness and perceptions, as well as barriers to its use. Participants were recruited from seven academic centers offering gender-affirming care to TGNB AYA across the United States.

Results

Of the 156 TGNB AYA individuals who completed the survey, most (67%) were aware of PrEP; however, few (7%) had been prescribed PrEP. Many (60%) had not spoken to a medical provider and, even if the medication was free and obtained confidentially, most participants did not plan to take PrEP due to low perceived HIV risk, lack of PrEP knowledge, and concern about interactions between their hormone therapy and PrEP.

Discussion

These findings underscore the need for broad PrEP educational efforts for both TGNB AYA and their providers to improve knowledge, identify potential PrEP candidates among TGNB AYA and improve access by addressing identified barriers.
Why Is the Baby Sunbathing? An Overview of Jaundice
Authors: Bhatia Megha , Kadamba Sasha , De Guia Rose Angeline , Ansari Hiba , Osier Nico
Published: January 2024

Have you ever seen a small baby with yellow skin or eyes? These babies have a skin condition called jaundice and it is actually pretty common. Jaundice occurs when too much of a waste substance called bilirubin stays in the blood for too long. Bilirubin is produced when red blood cells naturally break down and, in high amounts, it can cause damage to the body. The most common way doctors identify jaundice is the finger test: gently touching the baby’s skin with their finger to look for any yellowing. If the baby does have jaundice, there are two main treatments: ultraviolet (UV) light therapy and exchange transfusion therapy. Both work by bringing bilirubin in the blood down to normal levels.
Where Did Nap Time Go? Why Older Kids Do Not Nap at School
Authors: Babu Ganesh Darshini , Pandey Meghna , Riggins Tracy , Spencer Rebecca M. C. , Tiwari Rhea , Wehland Mark , Leikin Sonya
Published: January 2024

Getting enough sleep is important not only for our health but also for learning. If sleep is good for us, why do children stop napping as they get older? Why do some children stop napping around their second birthday while others nap much longer? To answer these questions, scientists reviewed studies on how sleep, the brain, and memory develop. They took information from each area to create a new theory about why and when children stop napping. The scientists suggested a specific “memory area” in the brain, known as the hippocampus, develops as children grow up. Once the hippocampus can store the day’s memories, it results in fewer “napping” signals sent to the body, causing fewer naps. Information about how and why children stop napping is important for parents and teachers so they can best support children’s sleep needs.
Long-read sequencing-based transcriptomic landscape in longissimus dorsi and transcriptome-wide association studies for growth traits of meat rabbits
Authors: Jia Xianbo , Kang Zhe , Wang Guozhi , Zhang Kai , Fu Xiangchao , Li Congyan , Lai Songjia , Chen Shi-Yi
Published: January 2024

Rabbits are an attractive meat livestock species that can efficiently convert human-indigestible plant biomass, and have been commonly used in biological and medical researches. Yet, transcriptomic landscape in muscle tissue and association between gene expression level and growth traits have not been specially studied in meat rabbits. In this study Oxford Nanopore Technologies (ONT) long-read sequencing technology was used for comprehensively exploring transcriptomic landscape in Longissimus dorsi for 115 rabbits at 84 days of age, and transcriptome-wide association studies (TWAS) were performed for growth traits, including body weight at 84 days of age and average daily gain during three growth periods. The statistical analysis of TWAS was performed using a mixed linear model, in which polygenic effect was fitted as a random effect according to gene expression level-based relationships. A total of 18,842 genes and 42,010 transcripts were detected, among which 35% of genes and 47% of transcripts were novel in comparison with the reference genome annotation. Furthermore, 45% of genes were widely expressed among more than 90% of individuals. The proportions (±SE) of phenotype variance explained by genome-wide gene expression level ranged from 0.501 ± 0.216 to 0.956 ± 0.209, and the similar results were obtained when explained by transcript expression level. In contrast, neither gene nor transcript was detected by TWAS to be statistically significantly associated with these growth traits. In conclusion, these novel genes and transcripts that have been extensively profiled in a single muscle tissue using long-read sequencing technology will greatly improve our understanding on transcriptional diversity in rabbits. Our results with a relatively small sample size further revealed the important contribution of global gene expression to phenotypic variation on growth performance, but it seemed that no single gene has an outstanding effect; this knowledge is helpful to include intermediate omics data for implementing genetic evaluation of growth traits in meat rabbits.
Sustainable Leadership and Hegelian Self-Awareness
Authors: Sebastian Anne , Hühn Matthias P.
Published: January 2024

The contribution of businesses to sustainability, captured in the concept of the triple bottom line (people, profit, planet), has been given a lot of attention. One line of argument is to make sustainability mandatory via regulation, while another is to create a consciousness for questions of sustainability in the minds of managers, that is, to make managers agents of sustainability. Thus, there is debate about what behaviors, practices, and skills leaders need for positive sustainability. In recent years, scholars have attempted to develop leadership approaches (e.g., sustainable leadership, sustainability leadership) that contribute to the implementation of sustainability and positively influence sustainability practices, filling a perceived gap in the current literature. Here, connections to popular relationship-based approaches such as ethical leadership, transformational leadership, authentic leadership, and responsible leadership are also discussed. In this paper, we debate to what extent a specific leadership type is necessary to successfully lead a company into a sustainable future or whether, regardless of conceptualized behaviors, practices, and skills, it is not the core character and attitude of leaders that is crucial to changing the company culture to achieve the goal of sustainable business. This paper first outlines the existing literature on leadership approaches designed to lead to greater sustainability. It does not claim to be exhaustive but refers to the most prominent research findings. It then critically assesses these and raises questions. In the third section, we propose an alternative view of successful leadership toward positive sustainability.
Consumers’ WTP for Sustainability Turfgrass Attributes with Consideration of Aesthetic Attributes and Water Conservation Policies
Authors: Jung Hyojae , Chung Chanjin
Published: January 2024

This study estimates consumers’ willingness to pay (WTP) for sustainability turfgrass attributes such as low-input and stress-tolerance attributes, while considering potential trade-off relationships between aesthetic attributes and sustainability attributes. To address our objectives, our study conducts a choice experiment and estimates two mixed logit models. The first model includes low-input, winter kill, and shade-tolerance attributes as predictor variables, and the second model extends the first model by adding interaction terms between the aesthetic and sustainability attributes. Another choice experiment is conducted under water policies with various water rate increase and watering restriction scenarios. Results from the mixed logit models show that, overall, higher low-input cost reduction, less winter-damaged, and more shade-tolerant grasses are preferred, and that the direct effect of aesthetic attributes on consumers’ preferences is strong, but the indirect effects represented by the interaction terms are generally statistically insignificant. Our results indicate that consumers like to have a pretty lawn, but no strong consideration is given to the aesthetics of their lawn when selecting low-input and stress-tolerant turfgrasses. Our choice experiment under water policy scenarios suggests that water pricing is more effective than watering restriction in increasing consumer demand for water-conserving turfgrasses.
Evaluation of Biochar Addition to Digestate, Slurry, and Manure for Mitigating Carbon Emissions
Authors: Verdi Leonardo , Dalla Marta Anna , Orlandini Simone , Maienza Anita , Baronti Silvia , Vaccari Francesco Primo
Published: January 2024

The contribution of animal waste storage on GHG emissions and climate change is a serious issue for agriculture. The carbon emissions that are generated from barns represent a relevant source of emissions that negatively affect the environmental performance measures of livestock production. In this experiment, CO2 and CH4 emissions from different animal wastes, namely, digestate, slurry, and manure, were evaluated both in their original form and with a biochar addition. The emissions were monitored using the static camber methodology and a portable gas analyzer for a 21-day period. The addition of biochar (at a ratio of 2:1 between the substrates and biochar) significantly reduced the emissions of both gases compared to the untreated substrates. Slurry exhibited higher emissions due to its elevated gas emission tendency. The biochar addition reduced CO2 and CH4 emissions by 26% and 21%, respectively, from the slurry. The main effect of the biochar addition was on the digestate, where the emissions decreased by 45% for CO2 and 78% for CH4. Despite a lower tendency to emit carbon-based gases of manure, biochar addition still caused relevant decreases in CO2 (40%) and CH4 (81%) emissions. Biochar reduced the environmental impacts of all treatments, with a GWP reduction of 55% for the digestate, 22% for the slurry, and 44% for the manure.
Metabolome and Transcriptome Analysis Provide Insights into Flower Bud Color Variation in the Adaptation to UV-B Radiation of Litchi
Authors: Xiao Zhidan , Wang Jing , Jiang Nonghui , Xiang Xu , Liu Wei
Published: January 2024

Flower color variations have increasingly been recognized as playing an important role in the adaptation to UV-B radiation; however, the underlying mechanism is poorly understood in perennial fruit trees. Litchi is an important fruit tree, and extremely early maturing (EEM) and middle-to-late-maturing (MLM) cultivars that originated from regions with high and low UV-B radiation have dark brown and light yellow flower buds, respectively, while their hybrid early-maturing (EM) cultivars have an intermediate brown flower bud. This study comprehensively analyzed the metabolome and transcriptome of flower buds of litchi EEM, EM and MLM cultivars to explore the mechanism underlying flower color variation during the adaptation to UV-B radiation for the first time. Metabolomic analysis identified 72 flavonoids in litchi flower buds, among which a higher accumulation of flavonol glycosides was responsible for darker flower buds of EEM cultivars. And transcriptome analysis revealed key structural genes, including LcCHI, LcFLS and seven UGTs, together with two transcription factors (LcMYB12 and LcMYB111), which could be directly up-regulated by UV-B radiation, playing critical roles in regulating the differential accumulation of flavonol glycosides. These results provide new insights into the molecular mechanism underlying adaptation to UV-B radiation and provide a genetic basis for future breeding of stress-tolerant cultivars of litchi.
Genome-Wide Identification of the Genes of the Odorant-Binding Protein Family Reveal Their Role in the Olfactory Response of the Tomato Leaf Miner (Tuta absoluta) to a Repellent Plant
Authors: Ma Ruixin , Li Donggui , Peng Chen , Wang Shuangyan , Chen Yaping , Gui Furong , Sun Zhongxiang
Published: January 2024

The remarkable biological and evolutionary adaptations of insects to plants are largely attributed to the powerful chemosensory systems of insects. The tomato leaf miner (Tuta absoluta) is a destructive invasive pest with a global distribution that poses a serious threat to the production of nightshade crops, especially tomatoes. Functional plants can attract or repel insect pests by releasing volatiles that interact with the olfactory system of insects, thereby reducing the damage of insect pests to target crops. However, there is limited research on the interaction between T. absoluta olfactory genes and functional plants. In this study, 97 members of the putative odorant-binding protein (OBP) family have been identified in the whole genome of T. absoluta. Phylogenetic analysis involving various Lepidopteran and Dipteran species, including D. melanogaster, revealed that OBP gene families present conserved clustering patterns. Furthermore, the Plus-C subfamily of OBP showed extremely significant expansion. Moreover, the expression levels of the OBP genes varied significantly between different developmental stages; that is, the highest number of OBP genes were expressed in the adult stage, followed by the larval stage, and fewer genes were expressed in high abundance in the egg stage. On the other hand, through a Y-tube olfactometer, we identified a functional plant—Plectranthus tomentosa—that significantly repels adult and larval T. absoluta. Finally, we screened the OBP genes in response to tomato and P. tomentosa volatiles at the genomic level of T. absoluta using RT-qPCR. These results laid a good foundation for controlling T. absoluta with functional plants and further studying olfactory genes.
Bovine Genital Leptospirosis: An Update of This Important Reproductive Disease
Authors: Aymée Luiza , Mendes Julia , Lilenbaum Walter
Published: January 2024

Bovine leptospirosis is an important disease that affects the reproductive sphere. Due to its high relevance for the bovine production chain in a worldwide scenario, a better understanding of the disease is crucial to reduce its negative impacts. The main agents are strains from the Sejroe serogroup, such as Hardjo and Guaricura, which lead to renal and genital infection. The genital colonization causes a chronic, silent, and subclinical reproductive syndrome, called Bovine Genital Leptospirosis (BGL). Embryonic death, estrus repetition, subfertility, and abortions are the main signs of BGL condition in females. However, although leptospires have been identified in semen, the manifestation of BGL in bulls remains to be clarified. The recommended diagnosis of BGL includes a serologic screening of the herds using the microscopic agglutination test followed by PCR of genital samples (cervicovaginal mucus, uterine fragment, or semen), especially from animals with reproductive failures. After the identification of carriers, control is carried out considering three steps: antimicrobial treatment of the carriers, environmental and reproductive management, and herd vaccination. Systematic testing, quarantine of newly arrived animals, and usage of antimicrobials in semen diluents or embryo culture media are other sanitary approaches that are encouraged to improve the control of the syndrome. Herein we discuss protocols for an efficient diagnosis and preventive procedures of BGL, which are fundamental to reducing the negative impact of the disease on cattle reproduction and its consequent economic hazards.
Genome-Wide Association Study of Milk Composition in Karachai Goats
Authors: Selionova Marina , Trukhachev Vladimir , Aibazov Magomet , Sermyagin Alexander , Belous Anna , Gladkikh Marianna , Zinovieva Natalia
Published: January 2024

This study is first to perform a genome-wide association study (GWAS) to investigate the milk quality traits in Karachai goats. The objective of the study was to identify candidate genes associated with milk composition traits based on the identification and subsequent analysis of all possible SNPs, both genome-wide (high-confidence) and suggestive (subthreshold significance). To estimate the milk components, 22 traits were determined, including several types of fatty acids. DNA was extracted from ear tissue or blood samples. A total of 167 Karachai goats were genotyped using an Illumina GoatSNP53K BeadChip panel (Illumina Inc., San Diego, CA, USA). Overall, we identified 167 highly significant and subthreshold SNPs associated with the milk components of Karachai goats. A total of 10 SNPs were located within protein-coding genes and 33 SNPs in close proximity to them (±0.2 Mb). The largest number of genome-wide significant SNPs was found on chromosomes 2 and 8 and some of them were associated with several traits. The greatest number of genome-wide significant SNPs was identified for crude protein and lactose (6), and the smallest number—only 1 SNP—for freezing point depression. No SNPs were identified for monounsaturated and polyunsaturated fatty acids. Functional annotation of all 43 SNPs allowed us to identify 66 significant candidate genes on chromosomes 1, 2, 3, 4, 5, 8, 10, 13, 16, 18, 21, 23, 25, 26, and 27. We considered these genes potential DNA markers of the fatty acid composition of Karachai goat milk. Also, we found 12 genes that had a polygenic effect: most of them were simultaneously associated with the dry matter content and fatty acids (METTL, SLC1A 8, PHACTR1, FMO2, ECI1, PGP, ABCA3, AMDHD2). Our results suggest that the genes identified in our study affecting the milk components in Karachai goats differed from those identified in other breeds of dairy goats.
An Overview of Reactive Oxygen Species Damage Occurring during In Vitro Bovine Oocyte and Embryo Development and the Efficacy of Antioxidant Use to Limit These Adverse Effects
Authors: Keane Jessica A. , Ealy Alan D.
Published: January 2024

The in vitro production (IVP) of bovine embryos has gained popularity worldwide and in recent years and its use for producing embryos from genetically elite heifers and cows has surpassed the use of conventional superovulation-based embryo production schemes. There are, however, several issues with the IVP of embryos that remain unresolved. One limitation of special concern is the low efficiency of the IVP of embryos. Exposure to reactive oxygen species (ROS) is one reason why the production of embryos with IVP is diminished. These highly reactive molecules are generated in small amounts through normal cellular metabolism, but their abundances increase in embryo culture because of oocyte and embryo exposure to temperature fluctuations, light exposure, pH changes, atmospheric oxygen tension, suboptimal culture media formulations, and cryopreservation. When uncontrolled, ROS produce detrimental effects on the structure and function of genomic and mitochondrial DNA, alter DNA methylation, increase lipid membrane damage, and modify protein activity. Several intrinsic enzymatic pathways control ROS abundance and damage, and antioxidants react with and reduce the reactive potential of ROS. This review will focus on exploring the efficiency of supplementing several of these antioxidant molecules on oocyte maturation, sperm viability, fertilization, and embryo culture.
Fermentation of Plant-Based Feeds with Lactobacillus acidophilus Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (Oreochromis niloticus) Reared in a Biofloc System
Authors: Neves Nataly Oliveira Dos Santos , De Dea Lindner Juliano , Stockhausen Larissa , Delziovo Fernanda Regina , Bender Mariana , Serzedello Letícia , Cipriani Luiz Augusto , Ha Natalia , Skoronski Everton , Gisbert Enric , Sanahuja Ignasi , Perez Fabregat Thiago El Hadi
Published: January 2024

This study evaluated the effect of fermentation with Lactobacillus acidophilus on the biochemical and nutritional compositions of a plant-based diet and its effects on the productive performance and intestinal health of juvenile Nile tilapia (Oreochromis niloticus) reared in a biofloc technology (BFT) system. The in vitro kinetics of feed fermentation were studied to determine the L. acidophilus growth and acidification curve through counting the colony-forming units (CFUs) mL−1 and measuring the pH. Physicochemical and bromatological analyses of the feed were also performed. Based on the microbial growth kinetics results, vegetable-based Nile tilapia feeds fermented for 6 (FPB6) and 18 (FPB18) h were evaluated for 60 days. Fermented diets were compared with a positive control diet containing fishmeal (CFM) and a negative control diet without animal protein (CPB). Fermentation with L. acidophilus increased lactic acid bacteria (LAB) count and the soluble protein concentration of the plant-based feed, as well as decreasing the pH (p < 0.05). FPB treatments improved fish survival compared with CPB (p < 0.05). Fermentation increased feed intake but worsened feed efficiency (p < 0.05). The use of fermented feeds increased the LAB count and reduced pathogenic bacteria both in the BFT system’s water and in the animals’ intestines (p < 0.05). Fermented plant-based feeds showed greater villi (FPB6; FPB18) and higher goblet cell (FPB6) counts relative to the non-fermented plant-based feed, which may indicate improved intestinal health. The results obtained in this study are promising and show the sustainable potential of using fermented plant-based feeds in fish feeding rather than animal protein and, in particular, fishmeal.
Impacts of Puppy Early Life Experiences, Puppy-Purchasing Practices, and Owner Characteristics on Owner-Reported Problem Behaviours in a UK Pandemic Puppies Cohort at 21 Months of Age
Authors: Brand Claire L. , O’Neill Dan G. , Belshaw Zoe , Dale Fiona C. , Merritt Bree L. , Clover Kathryn N. , Tay Mi-Xue Michelle , Pegram Camilla L. , Packer Rowena M. A.
Published: January 2024

Problem behaviours are a leading cause of relinquishment and euthanasia of young dogs. Previous research has identified associations between owner-reported problem behaviours and risk factors, including how dogs were acquired as puppies, early socialisation experiences, and owners’ experience levels. Puppies acquired during the 2020 phase of the UK COVID-19 pandemic (“Pandemic Puppies”) were more likely to be exposed to many of these risk factors compared to puppies acquired in 2019; however, consequences for their adult behaviour are, as yet, unexplored. This study aimed to investigate the impact of these early-life and provenance-based risk factors, in addition to owner management strategies (including training methods) into early adulthood, upon adult dog behaviour aged 21 months. An online longitudinal cohort study of n = 985 Pandemic Puppies was conducted, recruited from a cohort of n = 4369 puppies originally surveyed in November–December 2020, which included data on how and why they were acquired and their socialisation/habituation experiences <16 weeks of age. Nearly all owners (96.7%) reported that their dog had exhibited at least one problem behaviour up to 21 months of age (median: 5; IQR: 3–7). Almost one third of dogs (30.9%) were reported to have displayed separation-related behaviours. Multivariable linear regression modelling revealed that owners reporting more problem behaviours were more likely to use multiple aversive training techniques (e.g., physical punishment), the use of which was notably high in this population (82.3%) compared to previous studies. Attendance at online puppy classes was the sole protective factor against owner use of aversive training methods. Almost one third of owners had underestimated how hard training their dog would be; this view was more likely among first-time owners. Urgent efforts are required to support this vulnerable population of dogs, including providing owner education regarding humane training techniques, to improve canine emotional health and avoid future relinquishment and/or behavioural euthanasia.
miR-10a-5p Regulates the Proliferation and Differentiation of Porcine Preadipocytes Targeting the KLF11 Gene
Authors: Zhang Wanfeng , Zhao Tianzhi , Gao Xinyu , Ma Shuangji , Gong Tianye , Yang Yang , Li Meng , Cao Guoqing , Guo Xiaohong , Li Bugao
Published: January 2024

In the swine industry, meat quality, color, and texture are influenced by the excessive differentiation of fat cells. miRNAs have emerged as integral regulators of adipose development. This study delves into the influence of miR-10a-5b on the proliferation and differentiation of pig preadipocytes. Our findings reveal that miR-10a-5b is prevalent across various tissues. It hinders preadipocyte proliferation, amplifies the expression of adipogenic genes, promotes lipid accumulation, and, as a result, advances preadipocyte differentiation. We predict that KLF11 is the target gene of miRNA. A dual-fluorescence reporter assay was conducted to validate the binding sites of miR-10a-5b on the 3′UTR of the KLF11 mRNA. Results showed that miR-10a-5b targeted KLF11 3′UTR and reduced the fluorescence activity of the dual-fluorescent reporter vector. Our research also indicates that miR-10a-5b targets and downregulates the expression of both mRNA and the protein levels of KLF11. During the differentiation of the preadipocytes, KLF11 inhibited adipose differentiation and was able to suppress the promotion of adipose differentiation by miR-10a-5b. This underscores miR-10a-5b’s potential as a significant regulator of preadipocyte behavior by modulating KLF11 expression, offering insights into the role of functional miRNAs in fat deposition.
Fatty Acids in the Eggs of Red King Crabs from the Barents Sea
Authors: Dvoretsky Alexander G. , Bichkaeva Fatima A. , Baranova Nina F. , Dvoretsky Vladimir G.
Published: January 2024

The red king crab, Paralithodes camtschaticus, was introduced into the Barents Sea where, after a period of 30 years of adaptation, it has established a new population. This population has been commercially exploited over the past two decades, supporting profitable fisheries in both Russia and Norway. Biochemical studies aimed at assessing fatty acid profiles have been conducted, focusing primarily on the edible parts of red king crabs. Only recently have by-products been included in this research. Capture of female red king crabs is prohibited in Russia but is allowed in Norway. The fatty acids of the egg masses carried by these females have not yet been studied. To fill this knowledge gap, we assayed the fatty acid composition of eggs using gas–liquid chromatography. Our results showed a predominance of polyunsaturated fatty acids, while the concentrations of saturated and monounsaturated fatty acids were similar. Multivariate comparisons showed no significant differences in fatty acid profiles in terms of egg developmental stage (nauplius vs. metanauplius), habitat conditions (soft vs. hard bottoms), female size class, or number of autotomized limbs. However, individual comparisons showed some differences in fatty acids, the most important being the lower content of docosahexaenoic acid in eggs at the metanauplius stage compared to eggs at the nauplius stage, which is likely due to its essential role in the development of red king crab embryos. The total fatty acid content (53.94 mg g−1) was 2–87 times higher in eggs than in other red king crab tissues, confirming the critical role that fatty acids play in maintaining physiological processes during vitellogenesis. The high content of essential fatty acids and an optimal omega-3-to-omega-6 ratio (4.9) suggest that red king crab eggs are a good product for a healthy diet and a valuable source for extracting essential fatty acids.
Transcriptome Analysis and Identification of Cadmium-Induced Oxidative Stress Response Genes in Different Meretrix meretrix Developmental Stages
Authors: Xu Yiyuan , Wu Chenghui , Jin Jianyu , Tang Wenhan , Chen Yuting , Chang Alan Kueichieh , Ying Xueping
Published: January 2024

Cadmium (Cd) is one of the major pollutants in the aquatic environment, and it can easily accumulate in aquatic animals and result in toxic effects by changing the metabolism of the body, causing a serious impact on the immune system, reproductive system, and the development of offspring. The clam Meretrix meretrix is one of the commercially important species that is cultivated in large-scale aquaculture in China. To elucidate the underlying molecular mechanisms of Cd2+ in the developmental processes, fertilized eggs and larvae of M. meretrix at different developmental stages were exposed to Cd2+ (27.2 mg L−1 in natural seawater) or just natural seawater without Cd2+ (control), and high-throughput transcriptome sequencing and immunohistochemistry techniques were used to analyze the toxic effects of Cd on larvae at different early developmental stages. The results revealed 31,914 genes were differentially expressed in the different stages of M. meretrix development upon treatment with Cd2+. Ten of these genes were differentially expressed in all stages of development examined, but they comprised only six unigenes (CCO, Ndh, HPX, A2M, STF, and pro-C3), all of which were related to the oxidative stress response. Under Cd exposure, the expression levels of CCO and Ndh were significantly upregulated in D-shaped and pediveliger larvae, while pro-C3 expression was significantly upregulated in the fertilized egg, D-shaped larva, and pediveliger. Moreover, HPX, A2M, and STF expression levels in the fertilized egg and pediveliger larvae were also significantly upregulated. In contrast, CCO, Ndh, HPX, A2M, STF, and pro-C3 expression levels in the postlarva were all downregulated under Cd exposure. Besides the genes with changes in expression identified by the transcriptome, the expression of two other oxidative stress-related genes (MT and Nfr2) was also found to change significantly in the different developmental stages of M. meretrix upon Cd exposure, confirming their roles in combating oxidative stress. Overall, the findings of this study indicated that Cd would interfere with cellular respiration, ion transport, and immune response through inducing oxidative stress, and changes in the expression of oxidative stress-related genes might be an important step for M. meretrix to deal with the adverse effects of Cd at different stages of its development.
The Role of Adipokines in the Control of Pituitary Functions
Authors: Kaminska Barbara , Kurowicka Beata , Kiezun Marta , Dobrzyn Kamil , Kisielewska Katarzyna , Gudelska Marlena , Kopij Grzegorz , Szymanska Karolina , Zarzecka Barbara , Koker Oguzhan , Zaobidna Ewa , Smolinska Nina , Kaminski Tadeusz
Published: January 2024

The pituitary gland is a key endocrine gland in all classes of vertebrates, including mammals. The pituitary gland is an important component of hypothalamus–pituitary–target organ hormonal regulatory axes and forms a functional link between the nervous system and the endocrine system. In response to hypothalamic stimuli, the pituitary gland secretes a number of hormones involved in the regulation of metabolism, stress reactions and environmental adaptation, growth and development, as well as reproductive processes and lactation. In turn, hormones secreted by target organs at the lowest levels of the hormonal regulatory axes regulate the functions of the pituitary gland in the process of hormonal feedback. The pituitary also responds to other peripheral signals, including adipose-tissue-derived factors. These substances are a broad group of peptides known as adipocytokines or adipokines that act as endocrine hormones mainly involved in energy homeostasis. Adipokines, including adiponectin, resistin, apelin, chemerin, visfatin, and irisin, are also expressed in the pituitary gland, and they influence the secretory functions of this gland. This review is an overview of the existing knowledge of the relationship between chosen adipose-derived factors and endocrine functions of the pituitary gland, with an emphasis on the pituitary control of reproductive processes.
Evaluation of the Effect of an Olive Phenolic Extract on the Secondary Shelf Life of a Fresh Pesto
Authors: Sordini Beatrice , Urbani Stefania , Esposto Sonia , Selvaggini Roberto , Daidone Luigi , Veneziani Gianluca , Servili Maurizio , Taticchi Agnese
Published: January 2024

Recent advances in the olive oil sector aim to develop sustainable strategies for the valorisation of mechanical extraction co-products as a rich source of bioactive compounds with antioxidant and antimicrobial activities. In this work, we studied the effectiveness of a phenolic extract (PE) from olive vegetation water (OVW) as a new antioxidant of natural origin for improving the quality and extending the secondary shelf life (SSL) of a fresh basil pesto sold as a served loose product at the deli counter, simulating the storage conditions after packaging, opening, and serving. For that, the PE was mixed with the oily phase of fresh pesto in two different concentrations and compared to a control pesto (CTRL) made with the addition of common additives (ascorbic acid (E300) and sorbic acid (E200)). The physicochemical parameters, phenolic and volatile composition, sensory profiles, and antioxidant capacity of the experimental pesto samples were evaluated after opening. The results proved that the enrichment with the PE improved the stability of the pesto and, hence, its overall quality. The PE provided higher protection than the CTRL against primary and secondary oxidation at both concentrations tested and delayed the accumulation of the volatile compounds responsible for the ‘rancid’ off-flavour up to 7 days after first opening, while also preserving higher levels of the pesto phytonutrients (such as the rosmarinic, caffeic, and chicoric acids and α-tocopherol). These results show that the generation of food waste in households, catering chains, retail, and/or restaurants can be reduced, improving the sustainability of the food industry and the competitiveness of the olive oil sector.
Antimicrobial Effect of Submicron Complex Oxide Particles CsTeMoO6 under Visible Light
Authors: Semenycheva Lyudmila , Smirnov Vasiliy , Smirnova Olga , Shishkin Andrey , Anikina Nadezhda , Fukina Diana , Koryagin Andrey , Suleimanov Evgeny
Published: January 2024

The antimicrobial activity of submicron particles of new photocatalytic active complex metal oxide CsTeMoO6 against bacteria Escherichia coli and Staphylococcus aureus and fungi Aspergillus niger and Penicillium chrysogenum (spores and vegetative mycelium) was studied. It has been established that CsTeMoO6 has the antimicrobial activity in both under dark and visible light conditions in relation to all test cultures of microorganisms. The most inhibitory effect of CsTeMoO6 was noted for E. coli. The light enhanced the antimicrobial effect of the test compound against all cultures of bacteria and fungi, which is associated with the presence of photocatalytic activity of CsTeMoO6. The antifungal activity of CsTeMoO6 increased against spores and vegetative mycelium of fungi under light condition, and this effect increased with an increasing duration of time exposure. The different degree of survival rate of the studied microorganisms in the presence of this compound (under both dark and light) may be associated with the physiological and biochemical characteristics of the used microorganisms, including different mechanisms of resistance against complex metal oxide and reactive oxygen species.