bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary Study on Reconstruction of Building Thermal Field Based on Iterative Algorithm Acoustic CT
Authors: Qin Hengjie , Wen Jiangqi , Gao Zihe , Chai Lingling , Yao Haowei
Published: May 2023

Real-time acquisition and visualization of temperature anomalies in building spaces and 3D temperature field data during fires are of vital importance for fire danger warnings, early rescue operations, evacuation commands, and subsequent fire accident investigations. Taking into account the non-contact, global (planar and spatial), and high efficiency advantages of acoustic CT temperature measurement technology, this study involved the conducting of exploratory preliminary research in order to provide new ideas for the real-time global perception of information on building fires. The detailed research objective was as follows: obtain the temperature data at any time of a fire based on Fire Dynamics Simulator (FDS) and fit them to form the base temperature distribution diagram at that time. The large ill-conditioned matrix equation of acoustic flight under the scheme of multi-grid division was then constructed. The discrete temperature data of each grid in the building space was obtained by solving the matrix equation based on algebraic reconstruction algorithm (ART) and joint algebraic reconstruction algorithm (SART). The three-dimensional temperature field reconstruction of building space was realized by the interpolation of discrete temperature data. The reconstruction effect of each scheme was evaluated through the error analysis between the reconstruction data and the basic data. The results show that the real-time reconstruction of a 3D temperature field of a building thermal field can be realized based on acoustic CT temperature measurement technology, and the reconstruction algorithm and grid division scheme have a significant control effect on the reconstruction effect.
Upward Fire Spread Hazard of Vertical Greenery Systems: A Comparative Study with External Thermal Insulation Composite System and Double-Skin Façade
Authors: Karunaratne Tharindu Lakruwan Wickremanayake , Chow Cheuk Lun
Published: May 2023

Recent studies have shown that vertical greenery systems (VGS) carry a significant fire threat when not properly looked after. Building on this, the fire hazard of VGS was compared to two other thermally efficient building façade systems (TEBFS), namely external thermal insulation composite systems (ETICS) and double-skin façade (DSF). Numerical simulations were conducted in the fire dynamic simulator (FDS). A fire initiated as a room fire of 1 MW followed by a window-ejected flame on a 12 m tall and 9 m wide front façade with a TEBFS. Three scenarios for each TEBFS were simulated for better comparison. Rapid upward fire spread (UFS) was observed in the VGS scenarios, recording average UFS rates of 8.97, 5.51 and 2.86 cms−1 compared to the scenarios of the other 2 TEBFS where the flame failed to reach the top of the façade within the stipulated simulation time of 300 s. The maximum temperatures reached along the façade in VGS scenarios were much higher than those in the other two TEBFS. In conclusion, the fire hazard of VGS in certain conditions is much higher compared to the fire scenarios of ETICS and DSF that are scrutinised by building codes in many countries for fire safety.
Dried Vegetables as Potential Clean-Label Phosphate Substitutes in Cooked Sausage Meat
Authors: Weigel Ingrid , Nistler Sarah , Pichner Rohtraud , Budday Silvia , Gensberger-Reigl Sabrina
Published: May 2023

While phosphates are key additives in sausage production, their use conflicts with consumer preferences for “natural” foods. In this study, we investigated the potential of using vegetables as “clean-label” phosphate substitutes and their effects on water holding capacity, consumer acceptance, color, softness, and tenderness. Six freeze-dried vegetables with a pH above 6.0 were added to sausage meat on a laboratory scale. Adding 1.6% freeze-dried Brussels sprouts or Red Kuri squash resulted in a similar weight gain (7.0%) as the positive control of 0.6% commercial phosphate additive. Higher vegetable concentrations (2.2–4.0%) caused a significant increase in weight (p ≤ 0.05, 10.4–18.4% weight gain). Similar stress was needed to compress sausages containing 1.6/4.0% Brussels sprouts (14.2/11.2 kPa) and the positive control (13.2 kPa). Indentation tests also led to similar softness results for the sausages prepared with 1.6/4.0% Brussels sprouts (15.5 kPa/16.6 kPa) and the positive control (16.5 kPa). A force of 1.25 N was needed to shear the positive control, while 1.60 N/1.30 N was needed for the samples (1.6/4% Brussels sprouts). In summary, the present study indicates that freeze-dried vegetables have the potential to effectively replace phosphate in meat products.
Effects of pH and Ionic Strength in Calcium on the Stability and Aeration Characteristics of Dairy Emulsion
Authors: Wang Yunna , Cui Xin , Li Yang , Wang Shiran , Yan Guosen , Zhang Liebing , Li Yan
Published: May 2023

The effects of different pH levels and ionic strength in calcium on the stability and aeration characteristics of dairy emulsions were investigated in this study. The results revealed that the stability and aeration characteristics of the emulsion were enhanced as the pH value increased from 6.5 to 7.0 and were optimal within the pH of 6.8~7.0, while the concentration of free calcium ions (Ca2+) was 2.94~3.22 mM. With the pH subsequently fixed at 6.8 and 7.0, when the addition of CaCl2 was increased to 2.00 mM (free Ca2+ strength > 4.11 mM), stability and aeration characteristics reduced significantly, including the flocculation of fat globules, an increase in particle size, and a decrease in the zeta potential and viscosity of the O/W emulsion, all leading to an increase in interfacial protein mass and decreased overrun and foam firmness. Overall, the results indicated that pH changes and CaCl2 addition significantly influenced the stability and aeration characteristics of dairy emulsions, by influencing free Ca2+ strength, which is an important factor in determining the quality of dairy emulsions.
Editorial for the Special Issue “Advances in Colloidal Hydrogels”
Authors: Liu Fuguo , Ngai To
Published: May 2023

Hydrogels are three-dimensional polymer networks derived from hydrophilic macromonomers, which can be categorized as natural, synthetic, or hybrid hydrogels [...]
Sodium Salt of Partially Carboxymethylated Sodium Alginate-Graft-Poly(Acrylonitrile): II Superabsorbency, Salt Sensitivity and Swelling Kinetics of Hydrogel, H-Na-PCMSA-g-PAN
Authors: Trivedi Jignesh , Chourasia Arvind
Published: May 2023

The water absorption measurements of a novel superabsorbent anionic hydrogel, H-Na-PCMSA-g-PAN, has been reported first time in water with a poor conductivity, 0.15 M saline (NaCl, CaCl2, and AlCl3) solutions, and simulated urine (SU) solutions at various times. The hydrogel has been prepared by the saponification of the graft copolymer, Na-PCMSA-g-PAN (%G = 316.53, %GE = 99.31). Results indicated that as compared to the swelling capacity values evaluated in water with a poor conductivity, the ability of the hydrogel to swell in various saline solutions with the same concentration is significantly reduced at all different durations. The swelling tends to be Na+ > Ca2+ > Al3+ at the same saline concentration in the solution. Studies of the absorbency in various aqueous saline (NaCl) solutions also revealed that the swelling capacity decreased as the ionic strength of the swelling medium rose, which is consistent with the experimental results and Flory’s equation. Furthermore, the experimental results strongly suggested that second-order kinetics governs the swelling process of the hydrogel in various swelling media. The swelling characteristics and equilibrium water contents for the hydrogel in various swelling media have also been researched. The hydrogel samples have been successfully characterized by FTIR to show the change in chemical environment to COO− and CONH2 groups after swelling in different swelling media. The samples have also been characterized by SEM technique.
Germline JAK2 E846D Substitution as the Cause of Erythrocytosis?
Authors: Maaziz Nada , Garrec Céline , Airaud Fabrice , Bobée Victor , Contentin Nathalie , Cayssials Emilie , Rimbert Antoine , Aral Bernard , Bézieau Stéphane , Gardie Betty , Girodon François
Published: May 2023

The discovery in 2005 of the JAK2 V617F gain-of-function mutation in myeloproliferative neoplasms and more particularly in polycythemia vera has deeply changed the diagnostic and therapeutic approaches to polycythemia. More recently, the use of NGS in routine practice has revealed a large number of variants, although it is not always possible to classify them as pathogenic. This is notably the case for the JAK2 E846D variant for which for which questions remain unanswered. In a large French national cohort of 650 patients with well-characterized erythrocytosis, an isolated germline heterozygous JAK2 E846D substitution was observed in only two cases. For one of the patients, a family study could be performed, without segregation of the variant with the erythrocytosis phenotype. On the other hand, based on the large UK Biobank resource cohort including more than half a million UK participants, the JAK2 E846D variant was found in 760 individuals, associated with a moderate increase in hemoglobin and hematocrit values, but with no significant difference to the mean values of the rest of the studied population. Altogether, our data as well as UK Biobank cohort analyses suggest that the occurrence of an absolute polycythemia cannot be attributed to the sole demonstration of an isolated JAK2 E846D variant. However, it must be accompanied by other stimuli or favoring factors in order to generate absolute erythrocytosis.
Correction: Lauria, G.; Sineo, L. Human Peopling and Population Dynamics in Sicily: Preliminary Analysis of the Craniofacial Morphometric Variation from the Paleolithic to the Contemporary Age. Heritage 2023, 6, 1187–1208
Authors: Lauria Gabriele , Sineo Luca
Published: May 2023

In the original publication [...]
Yeast Mixtures for Postharvest Biocontrol of Diverse Fungal Rots on Citrus limon var Eureka
Authors: Edward-Rajanayagam Rose Meena Amirthanayagam , Narváez-Zapata José Alberto , Ramírez-González María del Socorro , de la Cruz-Arguijo Erika Alicia , López-Meyer Melina , Larralde-Corona Claudia Patricia
Published: May 2023

Mexico is among the most important citrus fruit producers in the world. However, during storage, several problems related to fungi can arise. The most common fungal postharvest diseases detected on Citrus limon var Eureka (Italian lime) produced in the Tamaulipas state are green/blue mold (Penicillium spp.), fusarium rot (F. oxysporum, F. solani, F. proliferatum, among others), and anthracnose (Colletotrichum spp.). In this work, we selected yeasts, occurring as the natural epiphytic mycoflora of lemons or from fermented traditional products, to be tested as part of a formulation for protecting stored lemons against fungal diseases. The best-performing yeasts, labeled as LCBG-03 (Meyerozyma guilliermondii), LCBG-30 (Pseudozyma sp.), and LCBG-49 (Saccharomyces cerevisiae), were selected to test their compatibility and biocontrol performance against strains of Penicillium digitatum (AL-38), Fusarium sp. (AL-21), Colletotrichum gloeosporioides (AL-13), and Epicoccum sorghinum (H3A). Based on their in vitro performance regarding the percentage of radial growth inhibition, both applied individually or as two yeasts mixed at equal cellular concentrations, the best combinations (containing M. guilliermondii formulated with either Pseudozyma sp. or S. cerevisiae) were selected with efficacies higher than 95% in both in vitro fungal radial growth rate inhibition and on stored lemon fruits. This work contributes to the search for compatible yeast combinations with the aim to diminish the fungal losses of citrus fruits using biocontrol for citrus postharvest protection.
Can Soil Cover Affect the Performance, Yield, and Quality of Creeping Fresh Market Tomato Hybrids?
Authors: Dalbianco Alessandro Bandeira , Santi Adalberto , Oliveira Regiane Cristina de , Borges Cristine Vanz , Daniel Diego Fernando , Trento Daiane Andréia , Dipple Fernanda Lourenço , Dallacort Rivanildo , Seabra Júnior Santino
Published: May 2023

Soil cover is a major factor in the cultivation of creeping tomatoes, especially for in natura consumption. This study aimed to determine the combination of a suitable tomato hybrid and soil cover, resulting in superior production performance and quality attributes of tomato fruits. Tomato hybrids (Fascínio and Thaíse) were cultivated on five soil cover types (uncovered soil, plastic mulch, sorghum, Sudan grass, and pearl millet). The data were submitted to a principal component analysis (PCA), considering all the variables, through Biplot graphical analysis. A Pearson correlation analysis was performed at the 5% significance level. For biomass production, pearl millet and Sudan grass were distinguished from the other soil covers, showing lower decomposition rates and, consequently, longer half-lives. Covering with plastic mulch provided higher total (111 t ha−1) and commercial (74.4 t ha−1) yields, regardless of the analyzed hybrid. According to the PCA, soil cover management influences the production and quality of tomato fruits, except for chemical and post-harvest characteristics, and maintains ideal levels for commercialization for all treatments. The Fascínio hybrid presented better production attributes, higher total and commercial fruit production per plant as well as fruits with larger sizes, especially when grown in plastic mulch cover. The Fascínio hybrid also presented fruits with higher levels of bioactive compounds (lycopene and β-carotene).
Ectopic Expression of AGAMOUS-like 18 from Litchi (Litchi chinensis Sonn.) Delayed the Floral Organ Abscission in Arabidopsis
Authors: Wang Fei , Liang Zhijian , He Zidi , Ma Xingshuai , Li Jianguo , Zhao Minglei
Published: May 2023

The regulation of abscission has a significant impact on fruit yield and quality. Thus, understanding the mechanisms underlying abscission, particularly identifying key genes, is critical for improving fruit crop breeding and cultivation practices. Here, to explore the key genes involved in litchi fruitlet abscission, the two closest homologs of AGAMOUS-like 15/18 (LcAGL15 and LcAGL18) were identified. During the litchi fruitlet abscission process, LcAGL15 expression was reduced, whereas LcAGL18 expression was increased at the abscission zone. The abscission of floral organs was unaffected by ectopic expression of LcAGL15 in Arabidopsis. Moreover, high expression of LcAGL18 significantly delayed the abscission process of floral organs, particularly the sepals. Overexpression of LcAGL18 in Arabidopsis consistently repressed the expression of abscission-related genes, including HAESA (HAE) and HAESA-LIKE2 (HSL2), and cell wall remodeling genes at the abscission zone. Furthermore, LcAGL18 was localized in the nucleus and acted as a transcriptional inhibitor. Collectively, these results suggest that AGL18 homologs have conserved functions in Arabidopsis and litchi, and that LcAGL18 might function as a key regulator in litchi fruitlet abscission.
Phytochemical Composition and Content of Red-Fleshed Grape Accessions
Authors: Lu Lizhen , Yang Yingzhen , Zhong Gan-Yuan , Liang Zhenchang , Cheng Lailiang
Published: May 2023

Red-fleshed grapes are important breeding resources, and study of the content and composition of phenolic compounds in red-fleshed grapes is lacking. In this study, the profiles of phenolic compounds in the whole berry, flesh, and peel of thirteen red-fleshed grape (Vitis) accessions were determined for two consecutive years. The content of total phenolic compounds ranged from 4.795 to 29.875 mg g−1 FW (fresh weight) in berry, from 1.960 to 12.593 mg g−1 FW in flesh, and from 17.067 to 60.182 mg g−1 FW in peel. As expected, anthocyanins were the main phenolic compounds, accounting for 90.4, 89.4, and 94.1% of the total phenolic compounds in berry, flesh, and peel, respectively. Flavanols accounted for 36.2% of the non-anthocyanin phenolic compounds in berry, 35.3% in flesh, and 38.3% in peel. In comparison, flavonols accounted for about 11.6, 5.7, and 15.8% of the non-anthocyanin phenolic compounds in berry, flesh, and peel, respectively. Hydroxycinnamic derivatives were the most abundant non-anthocyanins and accounted for 53.8, 56.1, and 44.3% of non-anthocyanin phenolic compounds in these three tissues. The content of phenolic compounds in peel was significantly higher than that in flesh and whole berry. A significant variation in the content of total and individual phenolic compounds was observed among different red-fleshed grapes, suggesting that genetic background was an important factor affecting the accumulation of these phenolic compounds. This work represents the most comprehensive characterization of phenolic compounds profiles in red-fleshed grapes.
The Efficacy of Two Group Interventions on Mental Representations, Attachment Security, and Trauma Symptoms in Ethnically and Socioeconomically Minoritized Young Adolescents in an Urban Middle School
Authors: Goodman Geoff , Blum Bryan , Rentrop Carla , Malberg Norka , Agrawal Palakrajiv
Published: May 2023

Symptoms resulting from childhood trauma can negatively impact socioemotional well-being and school performance during early adolescence unless positive changes in attachment security and mental representations of significant relationships occur. A sample of 109 eighth grade urban students were randomly assigned to one of two weekly, one-hour, school-based group interventions—Storytelling/Story-Acting for Adolescents (STSA-A) or Mentalization-Based Treatment Group Intervention (MBT-G). The Object Relations Inventory (ORI), Adolescent Attachment Questionnaire (AAQ) and Child PSTD Stress Scale (CPSS) were administered to students and their primary group leaders at the beginning (October) and end (May) of the intervention protocol as outcome variables. Participants in both the STSA-A and MBT-G intervention conditions experienced significant increases in attachment security and decreases in trauma symptoms. Over the course of eight months of group intervention, affective valence of paternal mental representations significantly decreased for boys and for participants in the STSA-A condition, while affective valence of primary group leader mental representations significantly decreased for participants in the MBT-G condition. STSA-A and MBT-G were found to be efficacious at improving attachment security and reducing trauma symptoms in young adolescents. The strengths of each group intervention for addressing interpersonal issues unique to specific types of adolescents are discussed.
Using Virtual Reality to Bring Restorative Environments to Employees: An Online Pilot Study
Authors: Horan Kristin A. , Harrington Maria , LeNoble Chelsea A. , Mosher Matthew , Pring Thomas
Published: May 2023

Employees face many demands throughout the workday. Participating in activities can help employees recover from the pressures of work, and physical activity and time spent in nature are among the most beneficial. Simulations of nature offer some of the benefits of actual contact with nature and can address some of the barriers to exercising outdoors that some employees may face. In this pilot study, we examine the influence of physical activity and virtual or actual nature contact on affect, boredom, and satisfaction when experienced during a break from a demanding work task. Twenty-five employed adults participated in an online study in which they completed a problem-solving task, completed a twenty-minute break, and then completed another session of the problem-solving task. During the break, participants were randomized to either a control condition, a physical activity and low-fidelity virtual nature contact condition, a physical activity and high-fidelity virtual nature contact condition, or a physical activity and actual nature contact condition. An examination of the means of affect, boredom, and satisfaction before, during, and after the break revealed that those in high-fidelity virtual nature and actual nature contact conditions seemed to report more positive well-being during the break. The results highlight that to help employees recover from work demands, it could be important to take breaks, be physically active, and have contact with nature, which should be simulated in high fidelity if actual nature contact cannot be achieved.
Understanding the Role of Therapy Dogs in Human Health Promotion
Authors: McDowall Sonya , Hazel Susan J. , Cobb Mia , Hamilton-Bruce Anne
Published: May 2023

Dogs may provide humans with a range of physical, mental and social benefits. Whilst there is growing scientific evidence of benefits to humans, there has been less focus on the impact to canine health, welfare and ethical considerations for the dogs. The importance of animal welfare is increasingly acknowledged, indicating that the Ottawa Charter should be extended to include the welfare of non-human animals supporting the promotion of human health. Therapy dog programmes are delivered across a variety of settings including hospitals, aged care facilities and mental health services, highlighting the important role they play in human health outcomes. Research has shown that that there are biomarkers for stress in humans and other animals engaged in human–animal interactions. This review aims to assess the impact of human–animal interactions on therapy dogs engaged in providing support to human health. While challenging, it is paramount to ensure that, within the framework of One Welfare, the welfare of therapy dogs is included, as it is a key factor for future sustainability. We identified a range of concerns due to the lack of guidelines and standards to protect the wellbeing of the dogs engaged in these programmes. Extension of the Ottawa Charter to include the welfare of non-human animals with leveraging through a One Welfare approach would promote animal and human health beyond current boundaries.
A Review of the Role of Pollen in COVID-19 Infection
Authors: Idrose Nur Sabrina , Zhang Jingwen , Lodge Caroline J. , Erbas Bircan , Douglass Jo A. , Bui Dinh S. , Dharmage Shyamali C.
Published: May 2023

There is current interest in the role of ambient pollen in the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2 or COVID-19) infection risk. The aim of this review is to summarise studies published up until January 2023 investigating the relationship between airborne pollen and the risk of COVID-19 infection. We found conflicting evidence, with some studies showing that pollen may increase the risk of COVID-19 infection by acting as a carrier, while others showed that pollen may reduce the risk by acting as an inhibiting factor. A few studies reported no evidence of an association between pollen and the risk of infection. A major limiting factor of this research is not being able to determine whether pollen contributed to the susceptibility to infection or just the expression of symptoms. Hence, more research is needed to better understand this highly complex relationship. Future investigations should consider individual and sociodemographic factors as potential effect modifiers when investigating these associations. This knowledge will help to identify targeted interventions.
Kitchen Characteristics and Practices Associated with Increased PM2.5 Concentration Levels in Zimbabwean Rural Households
Authors: Muteti-Fana Shamiso , Nkosana Jafta , Naidoo Rajen N.
Published: May 2023

Household air pollution (HAP) from biomass fuels significantly contributes to cardio-respiratory morbidity and premature mortality globally. Particulate matter (PM), one of the pollutants generated, remains the most accurate indicator of household air pollution. Determining indoor air concentration levels and factors influencing these levels at the household level is of prime importance, as it objectively guides efforts to reduce household air pollution. This paper describes household factors associated with increased PM2.5 levels in Zimbabwean rural household kitchens. Our HAP and lung health in women study enrolled 790 women in rural and urban households in Zimbabwe between March 2018 and December 2019. Here, we report data from 148 rural households using solid fuel as the primary source of fuel for cooking and heating and where indoor air samples were collected. Data on kitchen characteristics and practices were collected cross-sectionally using an indoor walk-through survey and a modified interviewer-administered questionnaire. An Air metrics miniVol Sampler was utilized to collect PM2.5 samples from the 148 kitchens over a 24 h period. To identify the kitchen features and practices that would likely influence PM2.5 concentration levels, we applied a multiple linear regression model. The measured PM2.5 ranged from 1.35 μg/m3 to 1940 μg/m3 (IQR: 52.1–472). The PM2.5 concentration levels in traditional kitchens significantly varied from the townhouse type kitchens, with the median for each kitchen being 291.7 μg/m3 (IQR: 97.2–472.2) and 1.35 μg/m3 (IQR: 1.3–97.2), respectively. The use of wood mixed with other forms of biomass was found to have a statistically significant association (p < 0.001) with increased levels of PM2.5 concentration. In addition, cooking indoors was strongly associated with higher PM2.5 concentrations (p = 0.012). Presence of smoke deposits on walls and roofs of the kitchens was significantly associated with increased PM2.5 concentration levels (p = 0.044). The study found that kitchen type, energy type, cooking place, and smoke deposits were significant predictors of increased PM2.5 concentrations in the rural households. Concentrations of PM2.5 were high as compared to WHO recommended exposure limits for PM2.5. Our findings highlight the importance of addressing kitchen characteristics and practices associated with elevated PM2.5 concentrations in settings where resources are limited and switching to cleaner fuels may not be an immediate feasible option.
Study on the Effect of EZH2 Inhibitor Combined with TIGIT Monoclonal Antibody against Multiple Myeloma Cells
Authors: Liu Zhaoyun , Jia Yue , Yang Chun , Liu Hui , Shen Hongli , Wang Hao , Fu Rong
Published: May 2023

EZH2, a member of the polycomb repressive complex 2, induces trimethylation of the downstream gene at the histone three lysine 27 (H3K27me3) position to inhibit tumor cell proliferation. Here, we showed that the apoptosis rate and apoptotic protein expression increased after EZH2 inhibition, whereas key molecules of the NF-κB signaling pathway and the downstream target genes were inhibited. Additionally, the expression of CD155, a TIGIT high-affinity ligand in multiple myeloma (MM) cells, was decreased by the mTOR signaling pathway. Furthermore, the combination of EZH2 inhibitor and TIGIT monoclonal antibody blockade enhanced the anti-tumor effect of natural killer cells. In summary, the EZH2 inhibitor not only plays an anti-tumor role as an epigenetic drug, but also enhances the anti-tumor effect of the TIGIT monoclonal antibody by affecting the TIGIT-CD155 axis between NK cells and MM cells, thus providing new ideas and theoretical basis for the treatment of MM patients.
Enzymatic and Bioinspired Systems for Hydrogen Production
Authors: Leone Linda , Sgueglia Gianmattia , La Gatta Salvatore , Chino Marco , Nastri Flavia , Lombardi Angela
Published: May 2023

The extraordinary potential of hydrogen as a clean and sustainable fuel has sparked the interest of the scientific community to find environmentally friendly methods for its production. Biological catalysts are the most attractive solution, as they usually operate under mild conditions and do not produce carbon-containing byproducts. Hydrogenases promote reversible proton reduction to hydrogen in a variety of anoxic bacteria and algae, displaying unparallel catalytic performances. Attempts to use these sophisticated enzymes in scalable hydrogen production have been hampered by limitations associated with their production and stability. Inspired by nature, significant efforts have been made in the development of artificial systems able to promote the hydrogen evolution reaction, via either electrochemical or light-driven catalysis. Starting from small-molecule coordination compounds, peptide- and protein-based architectures have been constructed around the catalytic center with the aim of reproducing hydrogenase function into robust, efficient, and cost-effective catalysts. In this review, we first provide an overview of the structural and functional properties of hydrogenases, along with their integration in devices for hydrogen and energy production. Then, we describe the most recent advances in the development of homogeneous hydrogen evolution catalysts envisioned to mimic hydrogenases.
Simultaneous Copy Number Alteration and Single-Nucleotide Variation Analysis in Matched Aqueous Humor and Tumor Samples in Children with Retinoblastoma
Authors: Schmidt Michael J. , Prabakar Rishvanth K. , Pike Sarah , Yellapantula Venkata , Peng Chen-Ching , Kuhn Peter , Hicks James , Xu Liya , Berry Jesse L.
Published: May 2023

Retinoblastoma (RB) is a childhood cancer that forms in the developing retina of young children; this tumor cannot be biopsied due to the risk of provoking extraocular tumor spread, which dramatically alters the treatment and survival of the patient. Recently, aqueous humor (AH), the clear fluid in the anterior chamber of the eye, has been developed as an organ-specific liquid biopsy for investigation of in vivo tumor-derived information found in the cell-free DNA (cfDNA) of the biofluid. However, identifying somatic genomic alterations, including both somatic copy number alterations (SCNAs) and single nucleotide variations (SNVs) of the RB1 gene, typically requires either: (1) two distinct experimental protocols—low-pass whole genome sequencing for SCNAs and targeted sequencing for SNVs—or (2) expensive deep whole genome or exome sequencing. To save time and cost, we applied a one-step targeted sequencing method to identify both SCNAs and RB1 SNVs in children with RB. High concordance (median = 96.2%) was observed in comparing SCNA calls derived from targeted sequencing to the traditional low-pass whole genome sequencing method. We further applied this method to investigate the degree of concordance of genomic alterations between paired tumor and AH samples from 11 RB eyes. We found 11/11 AH samples (100%) had SCNAs, and 10 of them (90.1%) with recurrent RB-SCNAs, while only nine out of 11 tumor samples (81.8%) had positive RB-SCNA signatures in both low-pass and targeted methods. Eight out of the nine (88.9%) detected SNVs were shared between AH and tumor samples. Ultimately, 11/11 cases have somatic alterations identified, including nine RB1 SNVs and 10 recurrent RB-SCNAs with four focal RB1 deletions and one MYCN gain. The results presented show the feasibility of utilizing one sequencing approach to obtain SCNA and targeted SNV data to capture a broad genomic scope of RB disease, which may ultimately expedite clinical intervention and be less expensive than other methods.
Carcino-Evo-Devo, A Theory of the Evolutionary Role of Hereditary Tumors
Authors: Kozlov Andrei P.
Published: May 2023

A theory of the evolutionary role of hereditary tumors, or the carcino-evo-devo theory, is being developed. The main hypothesis of the theory, the hypothesis of evolution by tumor neofunctionalization, posits that hereditary tumors provided additional cell masses during the evolution of multicellular organisms for the expression of evolutionarily novel genes. The carcino-evo-devo theory has formulated several nontrivial predictions that have been confirmed in the laboratory of the author. It also suggests several nontrivial explanations of biological phenomena previously unexplained by the existing theories or incompletely understood. By considering three major types of biological development—individual, evolutionary, and neoplastic development—within one theoretical framework, the carcino-evo-devo theory has the potential to become a unifying biological theory.
Structural Optimization and Interaction Study of a DNA Aptamer to L1 Cell Adhesion Molecule
Authors: Long Zhenhao , Bing Tao , Zhang Xiangru , Sheng Jing , Zu Shuang , Li Weiwei , Liu Xiangjun , Zhang Nan , Shangguan Dihua
Published: May 2023

The L1 cell adhesion molecule (L1CAM) plays important roles in the development and plasticity of the nervous system as well as in tumor formation, progression, and metastasis. New ligands are necessary tools for biomedical research and the detection of L1CAM. Here, DNA aptamer yly12 against L1CAM was optimized to have much stronger binding affinity (10–24 fold) at room temperature and 37 °C via sequence mutation and extension. This interaction study revealed that the optimized aptamers (yly20 and yly21) adopted a hairpin structure containing two loops and two stems. The key nucleotides for aptamer binding mainly located in loop I and its adjacent area. Stem I mainly played the role of stabilizing the binding structure. The yly-series aptamers were demonstrated to bind the Ig6 domain of L1CAM. This study reveals a detailed molecular mechanism for the interaction between yly-series aptamers and L1CAM and provides guidance for drug development and detection probe design against L1CAM.
Immunoinflammatory Background of Neuronal Damage in Stroke
Authors: Tuttolomondo Antonino
Published: May 2023

Ischemic stroke is caused by a reduction in blood flow to the brain and is a major cause of mortality and disability worldwide [...]
Exploring the Seasonal Dynamics and Molecular Mechanism of Wood Formation in Gymnosperm Trees
Authors: Nguyen Thi Thu Tram , Bae Eun-Kyung , Tran Thi Ngoc Anh , Lee Hyoshin , Ko Jae-Heung
Published: May 2023

Forests, comprising 31% of the Earth’s surface, play pivotal roles in regulating the carbon, water, and energy cycles. Despite being far less diverse than angiosperms, gymnosperms account for over 50% of the global woody biomass production. To sustain growth and development, gymnosperms have evolved the capacity to sense and respond to cyclical environmental signals, such as changes in photoperiod and seasonal temperature, which initiate growth (spring and summer) and dormancy (fall and winter). Cambium, the lateral meristem responsible for wood formation, is reactivated through a complex interplay among hormonal, genetic, and epigenetic factors. Temperature signals perceived in early spring induce the synthesis of several phytohormones, including auxins, cytokinins, and gibberellins, which in turn reactivate cambium cells. Additionally, microRNA-mediated genetic and epigenetic pathways modulate cambial function. As a result, the cambium becomes active during the summer, resulting in active secondary xylem (i.e., wood) production, and starts to become inactive in autumn. This review summarizes and discusses recent findings regarding the climatic, hormonal, genetic, and epigenetic regulation of wood formation in gymnosperm trees (i.e., conifers) in response to seasonal changes.
Structural-Activity Relationship of Rare Ginsenosides from Red Ginseng in the Treatment of Alzheimer’s Disease
Authors: Ye Xianwen , Zhang Haixia , Li Qian , Ren Hongmin , Xu Xinfang , Li Xiangri
Published: May 2023

Rare ginsenosides are the major components of red ginseng. However, there has been little research into the relationship between the structure of ginsenosides and their anti-inflammatory activity. In this work, BV-2 cells induced by lipopolysaccharide (LPS) or nigericin, the anti-inflammatory activity of eight rare ginsenosides, and the target proteins expression of AD were compared. In addition, the Morris water maze test, HE staining, thioflavins staining, and urine metabonomics were used to evaluate the effect of Rh4 on AD mice. Our results showed that their configuration influences the anti-inflammatory activity of ginsenosides. Ginsenosides Rk1, Rg5, Rk3, and Rh4 have significant anti-inflammatory activity compared to ginsenosides S-Rh1, R-Rh1, S-Rg3, and R-Rg3. Ginsenosides S-Rh1 and S-Rg3 have more pronounced anti-inflammatory activity than ginsenosides R-Rh1 and R-Rg3, respectively. Furthermore, the two pairs of stereoisomeric ginsenosides can significantly reduce the level of NLRP3, caspase-1, and ASC in BV-2 cells. Interestingly, Rh4 can improve the learning ability of AD mice, improve cognitive impairment, reduce hippocampal neuronal apoptosis and Aβ deposition, and regulate AD-related pathways such as the tricarboxylic acid cycle and the sphingolipid metabolism. Our findings conclude that rare ginsenosides with a double bond have more anti-inflammatory activity than those without, and 20(S)-ginsenosides have more excellent anti-inflammatory activity than 20(R)-ginsenosides.
The Role of Irisin/FNDC5 Expression and Its Serum Level in Breast Cancer
Authors: Cebulski Kamil , Piotrowska Aleksandra , Kmiecik Alicja , Haczkiewicz-Leśniak Katarzyna , Ciesielska Urszula , Grzegrzółka Jędrzej , Jabłońska Karolina , Romanowicz Hanna , Smolarz Beata , Dzięgiel Piotr , Podhorska-Okołów Marzenna , Nowińska Katarzyna
Published: May 2023

Irisin (Ir) is an adipomyokine formed from fibronectin type III domain-containing protein 5 (FNDC5), which can be found in various cancer tissues. Additionally, FNDC5/Ir is suspected of inhibiting the epithelial-mesenchymal transition (EMT) process. This relationship has been poorly studied for breast cancer (BC). The ultrastructural cellular localizations of FNDC5/Ir were examined in BC tissues and BC cell lines. Furthermore, we compared serum levels of Ir with FNDC5/Ir expression in BC tissues. The aim of this study was to examine the levels of EMT markers, such as E-cadherin, N-cadherin, SNAIL, SLUG, and TWIST, and to compare their expression levels with FNDC5/Ir in BC tissues. Tissue microarrays with 541 BC samples were used to perform immunohistochemical reactions. Serum levels of Ir were assessed in 77 BC patients. We investigated FNDC5/Ir expression and ultrastructural localization in MCF-7, MDA-MB-231, and MDA-MB-468 BC cell lines and in the normal breast cell line (Me16c), which was used as the control. FNDC5/Ir was present in BC cell cytoplasm and tumor fibroblasts. FNDC5/Ir expression levels in BC cell lines were higher compared to those in the normal breast cell line. Serum Ir levels did not correlate with FNDC5/Ir expression in BC tissues but were associated with lymph node metastasis (N) and histological grade (G). We found that FNDC5/Ir correlated moderately with E-cadherin and SNAIL. Higher Ir serum level is associated with lymph node metastasis and increased grade of malignancy. FNDC5/Ir expression is associated with E-cadherin expression level.
Ethyl Gallate Inhibits Bovine Viral Diarrhea Virus by Promoting IFITM3 Expression, Lysosomal Acidification and Protease Activity
Authors: Zhang Linlin , Yang Guanghui , Wang Jun , Zhang Jialu , Chen Keyuan , Xiong Xiaoran , Zhu Yaohong , Xu Chuang , Wang Jiufeng
Published: May 2023

Bovine viral diarrhea virus (BVDV) is a highly contagious viral disease which causes economic losses to the cattle industry. Ethyl gallate (EG) is a phenolic acid derivative which has various potentials to modulate the host response to pathogens, such as via antioxidant activity, antibacterial activity, inhibition of the production of cell adhesion factors, and so on. This study aimed to evaluate if EG influences BVDV infection in Madin-Darby Bovine Kidney (MDBK) cells, and to understand the antiviral mechanism. Data indicated that EG effectively inhibited BVDV infection by co-treatment and post-treatment in MDBK cells with noncytotoxic doses. In addition, EG suppressed BVDV infection at an early stage of the viral life cycle by blocking entry and replication steps but not viral attachment and release. Moreover, EG strongly inhibited BVDV infection by promoting interferon-induced transmembrane protein 3 (IFITM3) expression, which localized to the cytoplasm. The protein level of cathepsin B was significantly reduced by BVDV infection, whereas with treatment with EG, it was significantly enhanced. The fluorescence intensities of acridine orange (AO) staining were significantly decreased in BVDV-infected cells but increased in EG-treated cells. Finally, Western blot and immunofluorescence analyses demonstrated that EG treatment significantly enhanced the protein levels of autophagy markers LC3 and p62. Chloroquine (CQ) significantly increased IFITM3 expression, and Rapamycin significantly decreased it. Thus, EG may regulate IFITM3 expression through autophagy. Our results showed that EG could have a solid antiviral activity on BVDV replication in MDBK cells via increased IFITM3 expression, lysosomal acidification, protease activity, and regulated autophagy. EG might have value for further development as an antiviral agent.
Alterations in p53, Microsatellite Stability and Lack of MUC5AC Expression as Molecular Features of Colorectal Carcinoma Associated with Inflammatory Bowel Disease
Authors: Gené Míriam , Cuatrecasas Míriam , Amat Irene , Veiga Jesús Alberto , Fernández Aceñero María Jesús , Fusté Chimisana Victòria , Tarragona Jordi , Jurado Ismael , Fernández-Victoria Rebeca , Martínez Ciarpaglini Carolina , Alenda González Cristina , Zac Carlos , Ortega de la Obra Pilar , Fernández-Figueras María Teresa , Esteller Manel , Musulen Eva
Published: May 2023

Colitis-associated colorectal carcinoma (CAC) occurs in inflammatory bowel disease (IBD) because of the “chronic inflammation-dysplasia-cancer” carcinogenesis pathway characterized by p53 alterations in the early stages. Recently, gastric metaplasia (GM) has been described as the initial event of the serrated colorectal cancer (CRC) process, resulting from chronic stress on the colon mucosa. The aim of the study is to characterize CAC analyzing p53 alterations and microsatellite instability (MSI) to explore their relationship with GM using a series of CRC and the adjacent intestinal mucosa. Immunohistochemistry was performed to assess p53 alterations, MSI and MUC5AC expression as a surrogate for GM. The p53 mut-pattern was found in more than half of the CAC, most frequently stable (MSS) and MUC5AC negative. Only six tumors were unstable (MSI-H), being with p53 wt-pattern (p = 0.010) and MUC5AC positive (p = 0.005). MUC5AC staining was more frequently observed in intestinal mucosa, inflamed or with chronic changes, than in CAC, especially in those with p53 wt-pattern and MSS. Based on our results, we conclude that, as in the serrated pathway of CRC, in IBD GM occurs in inflamed mucosa, persists in those with chronic changes and disappears with the acquisition of p53 mutations.
Alternative Splicing Analysis Revealed the Role of Alpha-Linolenic Acid and Carotenoids in Fruit Development of Osmanthus fragrans
Authors: Ma Cancan , Zhang Cheng , Wang Xiaoyan , Zhu Fuyuan , Wang Xianrong , Zhang Min , Duan Yifan
Published: May 2023

Alternative splicing refers to the process of producing different splicing isoforms from the same pre-mRNA through different alternative splicing events, which almost participates in all stages of plant growth and development. In order to understand its role in the fruit development of Osmanthus fragrans, transcriptome sequencing and alternative splicing analysis was carried out on three stages of O. fragrans fruit (O. fragrans “Zi Yingui”). The results showed that the proportion of skipping exon events was the highest in all three periods, followed by a retained intron, and the proportion of mutually exclusive exon events was the lowest and most of the alternative splicing events occurred in the first two periods. The results of enrichment analysis of differentially expressed genes and differentially expressed isoforms showed that alpha-Linolenic acid metabolism, flavonoid biosynthesis, carotenoid biosynthesis, photosynthesis, and photosynthetic-antenna protein pathways were significantly enriched, which may play an important role in the fruit development of O. fragrans. The results of this study lay the foundation for further study of the development and maturation of O. fragrans fruit and further ideas for controlling fruit color and improving fruit quality and appearance.
BcMYB111 Responds to BcCBF2 and Induces Flavonol Biosynthesis to Enhance Tolerance under Cold Stress in Non-Heading Chinese Cabbage
Authors: Chen Xiaoshan , Wu Ying , Yu Zhanghong , Gao Zhanyuan , Ding Qiang , Shah Sayyed Hamad Ahmad , Lin Wenyuan , Li Ying , Hou Xilin
Published: May 2023

Flavonols have been shown to respond to a variety of abiotic stresses in plants, including cold stress. Higher total flavonoid content was found in non-heading Chinese cabbage (NHCC, Brassica campestris (syn. Brassica rapa) ssp. chinensis) after cold stress. A non-targeted metabolome analysis showed a significant increase in flavonol content, including that of quercetin and kaempferol. Here, we found that an R2R3–MYB transcription factor, BcMYB111, may play a role in this process. BcMYB111 was up-regulated in response to cold treatment, with an accompanying accumulation of flavonols. Then, it was found that BcMYB111 could regulate the synthesis of flavonols by directly binding to the promoters of BcF3H and BcFLS1. In the transgenic hairy roots of NHCC or stable transgenic Arabidopsis, overexpression of BcMYB111 increased flavonol synthesis and accumulation, while these were reduced in virus-induced gene silencing lines in NHCC. After cold stress, the higher proline content and lower malondialdehyde (MDA) content showed that there was less damage in transgenic Arabidopsis than in the wild-type (WT). The BcMYB111 transgenic lines performed better in terms of antioxidant capacity because of their lower H2O2 content and higher superoxide dismutase (SOD) and peroxidase (POD) enzyme activities. In addition, a key cold signaling gene, BcCBF2, could specifically bind to the DRE element and activate the expression of BcMYB111 in vitro and in vivo. The results suggested that BcMYB111 played a positive role in enhancing the flavonol synthesis and cold tolerance of NHCC. Taken together, these findings reveal that cold stress induces the accumulation of flavonols to increase tolerance via the pathway of BcCBF2–BcMYB111–BcF3H/BcFLS1 in NHCC.
The Moonlighting Function of Soybean Disordered Methyl-CpG-Binding Domain 10c Protein
Authors: Li Yanling , Qin Jiawei , Chen Menglu , Sun Nan , Tan Fangmei , Zhang Hua , Zou Yongdong , Uversky Vladimir N. , Liu Yun
Published: May 2023

Intrinsically disordered proteins (IDPs) are multifunctional due to their ability to adopt different structures depending on the local conditions. The intrinsically disordered regions of methyl-CpG-binding domain (MBD) proteins play important roles in regulating growth and development by interpreting DNA methylation patterns. However, whether MBDs have a stress-protective function is far from clear. In this paper, soybean GmMBD10c protein, which contains an MBD and is conserved in Leguminosae, was predicted to be located in the nucleus. It was found to be partially disordered by bioinformatic prediction, circular dichroism and a nuclear magnetic resonance spectral analysis. The enzyme activity assay and SDS-PAGE results showed that GmMBD10c can protect lactate dehydrogenase and a broad range of other proteins from misfolding and aggregation induced by the freeze–thaw process and heat stress, respectively. Furthermore, overexpression of GmMBD10c enhanced the salt tolerance of Escherichia coli. These data validate that GmMBD10c is a moonlighting protein with multiple functions.