bluuebunny/crossref_metadata_2025 huggingface dataset:
Aging-Related Moderation of the Link Between Compliance With International Physical Activity Recommendations and the Hemodynamic, Structural, and Functional Arterial Status of 3,619 Subjects Aged 3–90 Years
Authors: Zócalo Yanina , Gómez-García Mariana , Torrado Juan , Bia Daniel
Published: February 2022

Background

Compliance with physical activity recommendations (CPARs) is associated with better health indicators. However, there are only few studies to date that have comprehensively analyzed the association between CPARs and cardiovascular status “as a whole” (e.g., analyzing hemodynamic, structural, and functional properties, and different arterial territories). The relationship between CPARs and cardiovascular properties could be strongly influenced by the growth and aging process.

Aim

The goal of the study is to investigate the association between CPAR and cardiovascular properties by placing special emphasis on: (i) identifying if there is an independent association, (ii) if the association is “moderated” by age, and (iii) to what extent the association depends on the arterial parameter (hemodynamic vs. structural vs. functional) and/or the arterial segment (e.g., central vs. peripheral; elastic vs. transitional vs. muscular arteries).

Methods

A total of 3,619 subjects (3–90 years of age) were studied. Extensive cardiovascular evaluations were performed. Cardiovascular risk factors (CRFs) and physical activity (PA) levels were determined. The subjects were categorized as compliant (n = 1, 969) or non-compliant (n = 1,650) with World Health Organization-related PA recommendations. Correlation and multiple regression models (including CPAR*Age interaction) were obtained, and Johnson-Neyman technique was used to produce regions of significance.

Results

The independent association between CPARs and cardiovascular characteristics were strongly moderated by age. The moderation was observed on a wide range of age but particularly notorious on the extremes of life. Certain arterial characteristics demonstrated opposite effects in relation to CPAR status depending on the range of age considered. The association between CPAR and cardiovascular characteristics was independent of CRFs and moderated by age. In subjects younger than 45–55 years, CPAR status was associated with lower central and peripheral blood pressure (i.e., the younger the subject, the higher the reduction). During adult life, as age increases in the subjects, CPARs was associated with a beneficial hemodynamic profile, which is not related with variations in pressure but strongly related with lower levels of waveform-derived indexes and ventricular afterload determinants.

Conclusions

The independent associations between CPARs and arterial properties were strongly moderated by age. Data provided by blood pressure levels and waveform-derived indexes would be enough to evaluate the independent association between CPARs and the vascular system in the general population.
Environmental Concerns and Stewardship Behaviors Among Rural Landowners: What Supports Farmers and Non-farmers in Being Good Stewards?
Authors: Drescher Michael , Warriner G. Keith
Published: February 2022

Intensive agriculture is a main factor of biodiversity and ecosystem services loss globally. It is therefore of great importance to understand how rural landowners are managing their lands and how environmental stewardship behaviors could be strengthened. Farming and non-farming rural landowners are often considered a homogenous group. In reality, however, they vary by their histories, attitudes, interests, and resources. While many rural landowners manage their lands with environmental values in mind, others may struggle to do so. Ignoring this diversity poses the risk that planning and policy for sustainable agriculture are less effective than they could be. Hence, it is of interest to understand the variety of environmental perceptions and stewardship behaviors across these varied groups. To help addressing this knowledge gap, we conducted a survey of 1,200 farming and non-farming rural landowners, using Ontario as a case study. We specifically investigated whether farming landowners differed from non-farming landowners in expressed environmental concerns and stewardship behaviors, as well as what the roles are of participation in conservation incentive programs, demographic factors, and landholding characteristics. We analyzed survey answers with logistic regression and text analysis. Our results suggest that farming landowners are generally less environmentally concerned than non-farming landowners. However, it appears that this difference may be less driven by farm ownership than by contextual factors, such as landowner age and participation in conservation programs. Participation in conservation programs was more pronounced for non-farming landowners and was associated with higher likelihood of environmental concerns and engaging with stewardship behaviors. In contrast, higher age emerged as predictor of lower environmental concerns. In addition, we found that cost factors and knowledge needs were important barriers for stewardship behaviors across farming and non-farming rural landowners. Based on our results, we are making recommendations for increasing the effectiveness of agricultural sustainability planning and policy in Ontario, focusing on reducing financial and knowledge barriers to pro-environmental land management behaviors.
A Temporal Case-Based Reasoning Platform Relying on a Fuzzy Vector Spaces Object-Oriented Model and a Method to Design Knowledge Bases and Decision Support Systems in Multiple Domains
Authors: Colloc Joël , Yameogo Relwendé Aristide , Summons Peter , Loubet Lilian , Cavelier Jean-Bernard , Bridier Paul
Published: February 2022

Knowledge bases in complex domains must take into account many attributes describing numerous objects that are themselves components of complex objects. Temporal case-based reasoning (TCBR) requires comparing the structural evolution of component objects and their states (attribute values) at different levels of granularity. This paper provides some significant contributions to computer science. It extends a fuzzy vector space object-oriented model and method (FVSOOMM) to present a new platform and a method guideline capable of designing objects and attributes that represent timepoint knowledge objects. It shows how temporal case-based reasoning can use distances between temporal fuzzy vector functions to compare these knowledge objects’ evolution. It describes examples of interfaces that have been implemented on this new platform. These include an expert’s interface that describes a knowledge class diagram; a practitioner’s interface that instantiates domain objects and their attribute constraints; and an end-user interface to input attribute values of the real cases stored in a domain case database. This paper illustrates resultant knowledge bases in different domains, with examples of pulmonary embolism diagnosis in medicine and decision making in French municipal territorial recomposition. The paper concludes with the current limitations of the proposed model, its future perspectives and possible platform enhancements.
Robotic Sponge and Watercolor Painting Based on Image-Processing and Contour-Filling Algorithms
Authors: Scalera Lorenzo , Canever Giona , Seriani Stefano , Gasparetto Alessandro , Gallina Paolo
Published: February 2022

In this paper, the implementation of a robotic painting system using a sponge and the watercolor painting technique is presented. A collection of tools for calibration and sponge support operations was designed and built. A contour-filling algorithm was developed, which defines the sponge positions and orientations in order to color the contour of a generic image. Finally, the proposed robotic system was employed to realize a painting combining etching and watercolor techniques. To the best of our knowledge, this is the first example of robotic painting that uses the watercolor technique and a sponge as the painting media.
Effectiveness of Conflict Resolution Methods in Air Traffic Management
Authors: Dudoit Anrieta , Rimša Vytautas , Bogdevičius Marijonas , Skorupski Jacek
Published: February 2022

Aircraft fly en route under concurrent event situations (conflicting situations), which occur when they operate in the same airspace but are too close to each other in the same time frame. Hence, the safe horizontal distance between them is not less than the standard 5 nm. Free route airspace is such a concept, where such concurrent events need to be resolved when the location and number of such event “hotspots” are random in comparison with fixed route (conventional) airspace. This paper proposes two approaches to solving the traffic conflict in the sector by performing horizontal resolution maneuvers. The first of them uses the Dubins trajectory, while the second one uses a three-fold change of heading (3HC) method (for two types of angles). Apart from maintaining safe separation, we compared them, taking as a criterion the extension of the flight path of aircraft involved in the conflict, as the length of the flight is the primary factor determining flight time/delays and the increase in fuel consumption and greenhouse gas emissions. There may be other algorithms depending on the different data that can be identified through further research.
Digital Technology-and-Services-Driven Sustainable Transformation of Agriculture: Cases of China and the EU
Authors: Qin Tianyu , Wang Lijun , Zhou Yanxin , Guo Liyue , Jiang Gaoming , Zhang Lei
Published: February 2022

China’s sustainable development goals and carbon neutrality targets cannot be achieved without revolutionary transitions of the agricultural sector. The rapid development of digital technologies is believed to play a huge role in this revolution. The ongoing prevention and control of COVID-19 has greatly boosted the penetration of digital technology services in all areas of society, and sustainable transformation driven by digital technologies and services is rapidly becoming an area of innovation and research. Studies have shown that the rapid advancement of digitalization is also accompanied by a series of new governance challenges and problems: (1) unclear strategic orientation and inadequate policy and regulatory responses; (2) various stakeholders have not formed a sustainable community of interest; (3) information explosion is accompanied by information fragmentation and digital divide between countries and populations within countries. Meanwhile, current research has focused more on the role of digital services in urban governance and industrial development and lacks systematic research on its role in sustainable agricultural and rural development. To address the realities faced by different stakeholders in the process of digital transformation of agriculture, this paper aims to propose an inclusive analytical framework based on the meta-governance theory to identify and analyze the demand, supply, actor networks, and incentives in the digital technology-and-services-driven sustainable agricultural transformation, starting from the goals and connotations of sustainable agricultural and rural transformation and the interactions among different stakeholders in governing information flows. This analytical framework is further applied to analyze the cases of China and the EU. Although China and the EU represent different development phases and policy contexts, the framework is valid for capturing the characteristics of information flows and actor networks along the flows. It is concluded that a common information platform based on the stakeholder network would benefit all stakeholders, help reach common framing of issues, and maintain a dynamic exchange of information. Depending on the country context, different types of stakeholders may play different roles in creating, supervising, and maintaining such platforms. Digital infrastructures/products as hardware and farmers digital capacity as ‘software’ are the two wings for digital sustainable transformation. Innovative incentives from different countries may inspire each other. In any case, farmers’ actual farming behavior changes should be an important criterion for evaluating the effects and effectiveness of digital transition governance.
Alternative Solution to Synthetic Fertilizers for the Starter Fertilization of Bread Wheat under Mediterranean Climatic Conditions
Authors: Mathlouthi Fakir , Ruggeri Roberto , Rossini Francesco
Published: February 2022

Under the high temporal variability of the Mediterranean climate, the prompt emergence of wheat seedlings and early vigor are pivotal attributes, positively affecting the final grain yield. Phosphorus (P) fertilization at sowing is largely used in wheat cultivation to support the early-season growth and promote a more rapid crop establishment. While conventional farming can rely on a wide variety of synthetic fertilizers, the number of options available for the starter fertilization of organic wheat is restricted and many are often unsuitable. Nanotechnology applied to fertilizers could provide a valuable means to combat this issue. This study aimed to evaluate the starter effect of a granular soil bio-enhancer (SBE) on bread wheat grown in two locations of Northern Tunisia as compared to conventional fertilization with diammonium phosphate (DAP). The SBE was obtained by physically grinding phosphate rocks at a nanoscale level and further mixing it with azotobacters. Aerial dry biomass, plant height, crop density, tiller density, leaf area index (LAI), and leaf P concentration were determined up to 70 days after emergence (DAE) to assess the early vigor of wheat seedlings. The application of SBE before sowing resulted in a greater early vigor of wheat seedlings as compared to commercial DAP over three consecutive growing seasons. Specifically, the aerial dry biomass and the plant height at 7 DAE were found to have increased by 56% and 48%, respectively. LAI at 70 DAE was enhanced by 8.5%, while moderate percentage increases were detected for both crop and tiller density. Finally, wheat plants fertilized with SBE showed a significantly higher leaf P concentration than DAP-fertilized plants in 2020 and 2021 (+21% and +32%, respectively).
Effects of Closing Cut Date and Nitrogen Fertilization on Seed Yield and Seed Quality in Two Novel Cultivars of Urochloa spp.
Authors: Juntasin Weenaporn , Imura Yoshimi , Nakamura Ichiro , Hossain Mohammad Amzad , Thaikua Sarayut , Poungkaew Rattikan , Kawamoto Yasuhiro
Published: February 2022

Two field trials were conducted in Thailand to determine an appropriate closing cut date (CCD) and rate of nitrogen application (N-rate) to maximize seed yield and seed quality of the two novel cultivars (cv.) of Urochloa spp. (Synonym Brachiaria spp.), cv. ‘OKI-1’ (an open-pollinated tetraploid Urochloa ruziziensis (R. Germ. and C.M. Evrard) Crins originated from cv. ‘Miyaokikoku-ichigou’) and cv. ‘Br-203’ (U. ruziziensis cv. ‘Miyaokikoku-ichigou’ × U. hybrid cv. ‘Mulato’). The following treatments were evaluated in this study: four CCDs (uncut, 15 June, 1 July, and 15 July) and four N-rates (0, 50, 100, and 150 kg/ha). The cv. ‘OKI-1’ showed somewhat differences in tiller number/m2 (TN), fertile tiller percentage (FTP), inflorescence number/tiller (IN/T) and spikelet number/raceme (SN/R) with the CCD, while the cv. ‘Br-203’ showed only in SN/R. However, TN and SN/R were highest for 15 June, and FTP and IN/T were highest for 1 July in cv. ‘OKI-1’. The cv. ‘OKI-1’ showed the highest total seed yield (TSY), pure seed yield (PSY), and pure germinated seed yield (PGSY) for 1 July, followed by 15 June, and the cv. ‘Br-203’ showed the highest TSY, PSY, and PGSY for 15 July, followed by 1 July. Nitrogen (N) fertilization showed a negative effect on TSY for both the cultivars due to the higher N content in the soil. Withholding N fertilizer, a CCD in late-June to early-July and early-July to mid-July is recommended for cv. ‘OKI-1’ and cv. ‘Br-203’, respectively.
An Air Convection Wall with a Hollow Structure in Chinese Solar Greenhouses: Thermal Performance and Effects on Microclimate
Authors: Zhuang Yunfei , Zhao Shumei , Cheng Jieyu , Wang Pingzhi , Lu Na , Ma Chengwei , Xing Wenxin , Zheng Kexin
Published: February 2022

A Chinese solar greenhouse (CSG) is a horticultural facility that uses solar energy to promote a growth environment for crops and provides high-efficiency thermal storage performance to meet the demand of vegetables’ growth in winter. Besides being an important load-bearing structure in CSGs, the north wall is a heat sink, storing during the day in order to act as a heat source during the night. At times, the night temperature is lower than the minimum growth temperature requirement of vegetables, and the additional heating is needed. Therefore, optimizing the heat storage and release performance of the north wall in a CSG is an important approach for improving growth environment and reducing consumption of fossil fuel. This study proposes a heat storage north wall with a hollow layer on the basis of air convection, aiming to optimize the utilization of solar energy in CSGs. By the air convection effects, the hollow layer collects and stores surplus solar energy in the air during the day and transfers it to the cultivation space for heating at night. Additionally, field tests were conducted to compare the natural and forced convection strategies via airflow and heat transfer efficiency. The final effect on the indoor temperature ensured that the lowest temperatures at night were above 5 °C under both the natural and forced convection strategies during the winter in the Beijing suburbs where the average minimum temperature is below −10.8 °C during the experimental period. The hollow structure improves the utilization efficiency of solar energy in CSGs and ensures winter production efficiency in northern China.
Biodisinfection as a Profitable Fertilization Method for Horticultural Crops in the Framework of the Circular Economy
Authors: Castillo-Díaz Francisco José , Belmonte-Ureña Luis Jesús , Camacho-Ferre Francisco , Tello Marquina Julio César
Published: February 2022

Intensive agriculture has resulted in various environmental impacts that affect ecosystems. In some cases, the application of conventional fertilizers has deteriorated water quality, which includes the marine environment. For this reason, institutions have designed various strategies based on the principles of the circular economy and the bioeconomy. Both of these dynamics aim to reduce excessive fertilization and to inhibit the negative externalities it generates. In our work, a field trial is presented in which a 100% reduction in conventional inorganic fertilizers has been evaluated through a production methodology based on fertilization with reused plant debris in combination with other organic compounds. Based on one tomato crop, the profitability of this production technique has been analyzed in comparison with other conventional vegetable production techniques. The productivity and economic yield of the alternative crop was similar to that of the conventional crop, with a 37.2% decrease in water consumption. The reuse of biomass reduced production costs by 4.8%, while the addition of other organic amendments increased them by up to 22%. The results of our trial show that farms are more sustainable and more profitable from a circular point of view when using these strategies.
Utilizing Genomic Selection for Wheat Population Development and Improvement
Authors: Merrick Lance F. , Herr Andrew W. , Sandhu Karansher S. , Lozada Dennis N. , Carter Arron H.
Published: February 2022

Wheat (Triticum aestivum L.) breeding programs can take over a decade to release a new variety. However, new methods of selection, such as genomic selection (GS), must be integrated to decrease the time it takes to release new varieties to meet the demand of a growing population. The implementation of GS into breeding programs is still being explored, with many studies showing its potential to change wheat breeding through achieving higher genetic gain. In this review, we explore the integration of GS for a wheat breeding program by redesigning the traditional breeding pipeline to implement GS. We propose implementing a two-part breeding strategy by differentiating between population improvement and product development. The implementation of GS in the product development pipeline can be integrated into most stages and can predict within and across breeding cycles. Additionally, we explore optimizing the population improvement strategy through GS recurrent selection schemes to reduce crossing cycle time and significantly increase genetic gain. The recurrent selection schemes can be optimized for parental selection, maintenance of genetic variation, and optimal cross-prediction. Overall, we outline the ability to increase the genetic gain of a breeding program by implementing GS and a two-part breeding strategy.
Trapping of Ceratitis capitata Using the Low-Cost and Non-Toxic Attractant Biodelear
Authors: Kouloussis Nikos A. , Mavraganis Vassilis G. , Damos Petros , Ioannou Charalampos S. , Bempelou Eleftheria , Koveos Dimitris S. , Papadopoulos Nikos T.
Published: February 2022

Trapping is considered a powerful tool in the monitoring and control of fruit flies of high economic importance such as the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae). However, the cost of trapping and, in some cases, the safety of the chemicals used as baits are concerning for growers and the environment. Here we present a novel, low cost, environmentally friendly, female-specific bait for C. capitata, called Biodelear, that consists of a mixture of attracting compounds such us pyrazines, pyranones and amorphous nitrogen-based polymers. The new bait was compared to the commercially available attractant Biolure® (Suterra LLC, Bend, OR, USA) in Greece. McPhail-type traps were deployed in an orange orchard located in Athens. Five traps per treatment were used for several weeks during 2009 and four traps per treatment in 2010. Traps contained either 17 g of Biodelear or one Biolure Unipack dispenser. The results showed that both baits were highly efficient in attracting C. capitata females, and to a lesser extent, males. Although Biolure initially appeared to outperform Biodelear, later in the season the two attractants converged in efficacy. In both years, female captures were similar in traps baited with Biolure and Biodelear. However, male captures were higher in Biodelear-baited traps in 2010. In addition, Biodelear seemed to be longer lasting than Biolure, despite not being formulated into a slow-release dispersion system. The low cost of Biodelear and its strong, long-lasting effects render it suitable for mass trapping of the Mediterranean fruit fly.
Potentiation of β-Lactams against Methicillin-Resistant Staphylococcus aureus (MRSA) Using Octyl Gallate, a Food-Grade Antioxidant
Authors: Tamang Migma Dorji , Bae Junghee , Park Myungseo , Jeon Byeonghwa
Published: February 2022

Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to a number of antibiotics of clinical importance and is a serious threat to public health. Since bacteria rapidly develop resistance even to newly discovered antibiotics, this study aimed to develop drug potentiators to enhance the antibacterial activity of existing antibiotics for the control of MRSA. Based on our previous studies, screening of antimicrobial synergy was conducted with gallic acid and its derivatives using checkerboard assays. Antimicrobial synergy was confirmed with MRSA isolates from clinical cases. Combinations of penicillin, ampicillin, and cephalothin with octyl gallate (OG), an antioxidant approved by the US Food and Drug Administration (FDA), consistently exhibited synergistic bacteriostatic and bactericidal activities against MRSA, rendering MRSA sensitive to β-lactams. The fractional inhibitory concentration (FIC) and fractional bactericidal concentration (FBC) indices exhibited that the antimicrobial effects of OG were synergistic. The results of a permeability assay showed that OG significantly increased the permeability of the bacterial cell wall. Despite the intrinsic resistance of MRSA to β-lactams, the findings in this study demonstrated that OG enhanced the activity of β-lactams in MRSA and sensitized MRSA to β-lactams, suggesting that OG can be used as a drug potentiator to control MRSA using existing antibiotics.
Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life
Authors: Martins Garcia Martins , van Roest Manon , Vermeulen Jacqueline L. M. , Meisner Sander , Koster Jan , Wildenberg Manon E. , van Elburg Ruurd , Muncan Vanesa , Renes Ingrid B.
Published: February 2022

The association between prolonged antibiotic (AB) use in neonates and increased incidence of later life diseases is not yet fully understood. AB treatment in early life alters intestinal epithelial cell composition, functioning, and maturation, which could be the basis for later life health effects. Here, we investigated whether AB-induced changes in the neonatal gut persisted up to adulthood and whether early life AB had additional long-term consequences for gut functioning. Mice received AB orally from postnatal day 10 to 20. Intestinal morphology, permeability, and gene and protein expression at 8 weeks were analyzed. Our data showed that the majority of the early life AB-induced gut effects did not persist into adulthood, yet early life AB did impact later life gut functioning. Specifically, the proximal small intestine (SI) of adult mice treated with AB in early life was characterized by hyperproliferative crypts, increased number of Paneth cells, and alterations in enteroendocrine cell-specific gene expression profiles. The distal SI of adult mice displayed a reduced expression of antibacterial defense markers. Together, our results suggest that early life AB leads to structural and physiological changes in the adult gut, which may contribute to disease development when homeostatic conditions are under challenge.
Low Level of Colistin Resistance and mcr Genes Presence in Salmonella spp.: Evaluation of Isolates Collected between 2000 and 2020 from Animals and Environment
Authors: Bertelloni Fabrizio , Cagnoli Giulia , Turchi Barbara , Ebani Valentina Virginia
Published: February 2022

Salmonellosis is one of the most important zoonoses in Europe and the world. Human infection may evolve in severe clinical diseases, with the need for hospitalization and antimicrobial treatment. Colistin is now considered an important antimicrobial to treat infections from multidrug- resistant Gram-negative bacteria, but the spreading of mobile colistin-resistance (mcr) genes has limited this option. We aimed to evaluate colistin minimum inhibitory concentration and the presence of mcr (mcr-1 to mcr-9) genes in 236 Salmonella isolates previously collected from different animals and the environment between 2000 and 2020. Overall, 17.79% of isolates were resistant to colistin; no differences were observed in relation to years of isolation (2000–2005, 2009–2014, and 2015–2020), Salmonella enterica subspecies (enterica, salamae, diarizonae, and houtenae), origin of samples (domestic animals, wildlife, and environment), or animal category (birds, mammals, and reptiles); only recently isolated strains from houseflies showed the most resistance. Few isolates (5.93%) scored positive for mcr genes, in particular for mcr-1, mcr-2, mcr-4, mcr-6, and mcr-8; furthermore, only 2.54% of isolates were mcr-positive and colistin-resistant. Detected resistance to colistin was equally distributed among all examined Salmonella isolates and not always related to the presence of mcr genes.
Recent Advance in Small Molecules Targeting RhlR of Pseudomonas aeruginosa
Authors: Lim Taehyeong , Ham So-Young , Nam SangJin , Kim Myoungsun , Lee Ki Yong , Park Hee-Deung , Byun Youngjoo
Published: February 2022

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic gram-negative pathogen that can cause various infections, particularly in patients with compromised host defenses. P. aeruginosa forms biofilms and produces virulence factors through quorum sensing (QS) network, resulting in resistance to antibiotics. RhlI/RhlR, one of key QS systems in P. aeruginosa, is considered an attractive target for inhibiting biofilm formation and attenuating virulence factors. Several recent studies examined small molecules targeting the RhlI/RhlR system and their in vitro and in vivo biological activities. In this review, RhlR-targeted modulators, including agonists and antagonists, are discussed with particular focus on structure-activity relationship studies and outlook for next-generation anti-biofilm agents.
Perspective, Opportunities and Challenges in Using Fennel (Foeniculum vulgare) in Poultry Health and Production as an Eco-Friendly Alternative to Antibiotics: A Review
Authors: Khan Rifat Ullah , Fatima Adia , Naz Shabana , Ragni Marco , Tarricone Simona , Tufarelli Vincenzo
Published: February 2022

Following the European Union’s restriction on antibiotic growth promoters, research on enhancing gut health has been accelerated. As the poultry industry is facing issues that were previously managed by antimicrobial growth promoters, the hunt for the best remedies continues to find suitable alternatives. Simultaneously, social pressure is mounting to reduce the usage of antibiotics and replace them with other feed additives. Consumers believe a number of accessible options to be safe, with phytogenics playing a crucial role. This review describes how the use of fennel seeds could be beneficial for poultry. An overview of the broad chemical diversity of fennel is presented together with their physicochemical and biological properties. According to investigations, fennel seeds have a variety of biological effects in birds, including improved performance, higher immune cell proliferation, reduced oxidative stress, and boosted antibody titers against infectious diseases. The efficacy of poultry outcomes is determined by the stage and age of the plants, the extraction process, the geographical location, the chicken species, management techniques, and the concentrations administered. The present review focuses on the effects of fennel seeds as a feed additive on poultry growth, carcass quality, blood biochemistry, antioxidant activity, immunity, and microbiological aspects.
Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance
Authors: Catalán Jaime , Yánez-Ortiz Iván , Tvarijonaviciute Asta , González-Arostegui Luis Guillermo , Rubio Camila P. , Yeste Marc , Miró Jordi , Barranco Isabel
Published: February 2022

This study investigated whether the activities of the antioxidant components of donkey seminal plasma (SP)—both enzymatic (superoxide dismutase (SOD), catalase-like (CAT), glutathione peroxidase-like (GPX), and paraoxonase type 1 (PON1)) and non-enzymatic (measured in terms of total thiol, copper-reducing antioxidant capacity (CUPRAC), ferric-reducing ability of plasma (FRAP), and Trolox equivalent antioxidant capacity (TEAC))—and oxidative stress index (OSI) are related to sperm cryotolerance. For this purpose, 15 ejaculates from jackasses (one per individual) were collected and split into two aliquots. The first one was used for measuring the activities levels of enzymatic and non-enzymatic antioxidants and OSI in SP, whereas the other aliquot was cryopreserved. Before cryopreservation, sperm quality parameters (concentration, motility, and viability) were evaluated. After thawing, sperm motility, plasma membrane integrity, lipid disorder, mitochondrial membrane potential, reactive oxygen species (ROS), and calcium intracellular levels were also determined. Based on the percentages of total motility (TM) and of sperm with an intact plasma membrane (SYBR14+/PI−) after thawing, samples were classified as good-freezability (GFE) or poor-freezability (PFE) ejaculates through cluster analyses. The SP activity levels of enzymatic (SOD and PON1) and non-enzymatic antioxidants (CUPRAC, FRAP, and TEAC) were higher (p < 0.05) in GFE than in PFE, whereas SP-OSI was higher (p < 0.05) in PFE than in GFE. In addition, the activity levels of SOD, PON1, GPX, CUPRAC, FRAP, and TEAC were positively (p < 0.05) related to post-thaw sperm motility and plasma membrane integrity and negatively to intracellular ROS levels. The SP-OSI was negatively correlated (p < 0.05) to post-thaw sperm quality parameters and positively to intracellular ROS levels. It can thus be concluded that donkey SP antioxidants are related to sperm cryotolerance and that measurements of antioxidants PON1, SOD, CUPRAC, FRAP, and TEAC, as well as SP-OSI, could be used as markers of sperm cryotolerance. Further research addressing the relationship of these antioxidants and SP-OSI with sperm cryotolerance and their potential use as freezing markers is warranted.
CoenzymeQ10 and Ischemic Preconditioning Potentially Prevent Tourniquet-Induced Ischemia/Reperfusion in Knee Arthroplasty, but Combined Pretreatment Possibly Neutralizes Their Beneficial Effects
Authors: Leurcharusmee Prangmalee , Sawaddiruk Passakorn , Punjasawadwong Yodying , Sugundhavesa Nantawit , Klunklin Kasisin , Tongprasert Siam , Sitilertpisan Patraporn , Jaiwongkam Thidarat , Apaijai Nattayaporn , Chattipakorn Nipon , Chattipakorn Siriporn C.
Published: February 2022

Tourniquet (TQ) use during total knee arthroplasty (TKA) induces ischemia/reperfusion (I/R) injury, resulting in mitochondrial dysfunction. This study aims to determine the effects of coenzyme Q10 (CoQ10) and ischemic preconditioning (IPC), either alone or in combination, on I/R-induced mitochondrial respiration alteration in peripheral blood mononuclear cells (PBMCs) and pain following TKA. Forty-four patients were allocated into four groups: control, CoQ10, IPC, and CoQ10 + IPC. CoQ10 dose was 300 mg/day for 28 days. IPC protocol was three cycles of 5/5-min I/R time. Mitochondrial oxygen consumption rates (OCRs) of PBMCs were measured seven times, at baseline and during ischemic/reperfusion phases, with XFe 96 extracellular flux analyzer. Postoperative pain was assessed for 48 h. CoQ10 improved baseline mitochondrial uncoupling state; however, changes in OCRs during the early phase of I/R were not significantly different from the placebo. Compared to ischemic data, IPC transiently increased basal OCR and ATP production at 2 h after reperfusion. Clinically, CoQ10 significantly decreased pain scores and morphine requirements at 24 h. CoQ10 + IPC abolished analgesic effect of CoQ10 and mitochondrial protection of IPC. In TKA with TQ, IPC enhanced mitochondrial function by a transient increase in basal and ATP-linked respiration, and CoQ10 provides postoperative analgesic effect. Surprisingly, CoQ10 + IPC interferes with beneficial effects of each intervention.
Neurolocomotor Behavior and Oxidative Stress Markers of Thiazole and Thiazolidinedione Derivatives against Nauphoeta cinerea
Authors: Pereira Pedro Silvino , Costa Adrielle Rodrigues , de Oliveira Thalyta Julyanne Silva , Oliveira Carlos Vinícius Barros , de Lima Maria do Carmo Alves , de Oliveira Jamerson Ferreira , Kim Bonglee , Coutinho Henrique D. M. , Duarte Antonia Eliene , Kamdem Jean Paul , da Silva Teresinha Gonçalves
Published: February 2022

Thiazolidine compounds NJ20 {(E)-2-(2-(5-bromo-2-methoxybenzylidene)hydrazinyl)-4-(4-nitrophenyl)thiazole} and NW05 [(2-(benzo (d) (1,3) dioxol-4-ylmethylene)-N-(4-bromophenyl)-thiosemicarbazone] potentiated the effect of norfloxacin in resistant bacteria; however, there are no reports on their effects on Nauphoeta cinerea in the literature. The objective of this work was to evaluate the behavioral effects and oxidative markers of NW05 and NJ20 in lobster cockroach N. cinerea. To evaluate the behavioral study, a video tracking software was used to evaluate the locomotor points and the exploratory profile of cockroaches in the horizontal and vertical regions of a new environment. The total concentration of thiol and reduced glutathione (GSH), substances reactive to thiobarbituric acid (TBARS), free iron (II) content and mitochondrial viability were determined. The antioxidant potential was evaluated by the DPPH method. Both substances induced changes in the behavior of cockroaches, showing a significant reduction in the total distance covered and in the speed. In the cell viability test (MTT), there was a significant reduction for NJ20 (1 mM). NJ20 caused a significant increase in total levels of thiol and non-protein thiol (NPSH), although it also slightly increased the content of malondialdehyde (MDA). Both compounds (NW05 and NJ20) caused a significant reduction in the content of free iron at a concentration of 10 mM. In conclusion, the compound NJ20 caused moderate neurotoxicity (1 mM), but had good antioxidant action, while NW05 did not show toxicity or significant antioxidant activity in the model organism tested. It is desirable to carry out complementary tests related to the antioxidant prospection of these same compounds, evaluating them at different concentrations.
Medicarpin Increases Antioxidant Genes by Inducing NRF2 Transcriptional Level in HeLa Cells
Authors: Kim Jung-Hwan , Kang Dong-Min , Cho Young-Jin , Hyun Jin-Won , Ahn Mi-Jeong
Published: February 2022

The nuclear factor erythroid-derived 2-related factor 2 (NRF2) plays a pivotal role in the regulation of genes involved in oxidative stress and drug detoxification. Therefore, it is important to find NRF2 inducers to protect cells from excessive oxidative damage. Here, we investigated the effect of medicarpin isolated from the root of Robinia pseudoacacia L. on the activity of NRF2 in HeLa cells. Medicarpin significantly induced the antioxidant response elements (ARE)-luciferase activity in a concentration-dependent manner. Furthermore, medicarpin not only induced HO-1, GCLC, and NQO1 mRNA by translocating NRF2 to the nucleus but also induced the mRNA level of NRF2. To verify the NRF2 induction mechanism by medicarpin, ~2 kb of NRF2 promoter-luciferase assay was executed. As a result, medicarpin significantly induced NRF2-luciferase activity. Moreover, medicarpin strongly inhibited the ubiquitin-dependent proteasomal degradation of NRF2. Thus, medicarpin might protect cells by promoting the NRF2 transcriptional activity.
Lipophilic Grape Seed Proanthocyanidin Exerts Anti-Cervical Cancer Effects in HeLa Cells and a HeLa-Derived Xenograft Zebrafish Model
Authors: Li Changhong , Zhang Linli , Liu Chengmei , He Xuemei , Chen Mingshun , Chen Jun
Published: February 2022

Lipophilic grape seed proanthocyanidin (LGSP) synthesized from GSP and lauric acid exhibits an excellent antioxidant and anti-inflammatory effect. However, its anti-cervical cancer activity is still unknown. In this study, the in vitro anti-cervical cancer activity of LGSP on HeLa cell lines was investigated by MTT assay, flow cytometry and Western blot analysis, and its effect was explored by a HeLa-derived xenograft zebrafish model. LGSP exhibited an excellent anti-proliferative effect on HeLa cells by increasing the level of reactive oxygen species, which further induced cell apoptosis and blocked cell cycle progression in the G2/M phase. LGSP-treated HeLa cells showed a reduction in mitochondrial membrane potential, upregulation of the Bax/Bcl-2 ratio, release of cytochrome c into the cytoplasm, and activation of cleaved caspase-9/3 and cleavage of PARP, thus indicating that LGSP induced apoptosis through the intrinsic mitochondrial/caspase-mediated pathway. In the zebrafish model, LGSP effectively suppressed the growth of a HeLa xenograft tumor. These data suggest that LGSP may be a good candidate for the prevention or treatment of cervical cancer.
Effects of High-Intensity Electromagnetic Stimulation on Reducing Upper Limb Spasticity in Post-Stroke Patients
Authors: Ciortea Viorela Mihaela , Motoașcă Irina , Borda Ileana Monica , Ungur Rodica Ana , Bondor Cosmina Ioana , Iliescu Mădălina Gabriela , Ciubean Alina Deniza , Lazăr Izabela , Bendea Eliza , Irsay Laszlo
Published: February 2022

Super Inductive System (SIS) stimulation of spastic limbs by tissue-induced electromagnetic field may have the effect of reducing spasticity and improving functionality in patients with post-stroke spasticity. The aim of the study was to evaluate two different protocols for the application of SIS on upper limb spasticity after stroke. We included 60 patients with post-stroke upper limb spasticity, who were randomized into two groups: the study group, with a 9 min application protocol (1 min for agonist muscles, 8 min for antagonistic muscles); and the control group, with an 8 min protocol applied only to the antagonistic muscles. The duration of therapy was 10 days, and the results were assessed using the Modified Ashworth Scale (MAS) and the Barthel Index. Both the MAS and the Barthel Index improved significantly after 10 days of treatment (p < 0.001), but 30 days after the completion of therapy, there was an attenuation of the effects in both study groups. The study group had a significantly higher percentage of patients with improved MAS after 10 days (p = 0.004) and within 30 days (p < 0.001) than the control group. An SIS protocol applied on both agonist and antagonist muscles has a more pronounced and longer lasting spasticity-reducing and improved functionality effect than its application on only antagonistic muscles.
Long-Term Occurrence and Fate of Microplastics in WWTPs: A Case Study in Southwest Europe
Authors: Menéndez-Manjón Andrea , Martínez-Díez Reyes , Sol Daniel , Laca Amanda , Laca Adriana , Rancaño Amador , Díaz Mario
Published: February 2022

Microplastic (MP) water pollution is a major problem that the world is currently facing, and wastewater treatment plants (WWTPs) represent one of the main alternatives to reduce the MP release to the environment. Several studies have analysed punctual samples taken throughout the wastewater treatment line. However, there are few long-term studies on the evolution of MPs over time in WWTPs. This work analyses the performance of a WWTP sited in Southwest Europe in relation with annual occurrence and fate of MPs. Samples were monthly taken at different points of the facility (influent, secondary effluent, final effluent, and sludge) and MPs were quantified and characterised by means of stereomicroscopy and FTIR spectrophotometry. The majority of MPs found in wastewater and sludge samples were fragments and fibres. Regarding to the chemical composition, in the water samples, polyethylene (PE), polyethylene terephthalate (PET) and polypropylene (PP) stood out, whereas, in the sludge samples, the main polymers were PET, polyamide (PA) and polystyrene (PS). The MPs more easily removed during the wastewater treatment processes were those with sizes greater than 500 µm. Results showed that the MPs removal was very high during all the period analysed with removal efficiencies between 89% and 95%, so no great variations were found between months. MP concentrations in dry sludge samples ranged between 12 and 39 MPs/g, which represented around 79% of the total MPs removed during the wastewater treatment processes. It is noticeable that a trend between temperature and MPs entrapped in sewage sludge was observed, i.e., higher temperatures entailed higher percentage of retention.
Zero-Energy Buildings and Energy Efficiency towards Sustainability: A Bibliometric Review and a Case Study
Authors: Manzoor Bilal , Othman Idris , Sadowska Beata , Sarosiek Wiesław
Published: February 2022

The rapid growth of sustainability has created a plethora of options for expanding zero-energy buildings (ZEBs) and energy efficiency in all aspects of life. In recent years, there has been a rise in interest in ZEBs, and many countries have adopted ZEBs as future energy targets to promote the sustainable development paradigm. The primary goal of this paper was to conduct a bibliometric review of current research on ZEBs and energy efficiency. The first part of this paper identifies new knowledge gaps as well as practical demands in the field of sustainable development. Furthermore, bibliometric analysis was performed using the Scopus database (i.e., 2592 articles) and a screening process was undertaken, with the result being 252 papers. This study draws attention to a body of knowledge by reviewing trends and patterns, major research topics, journals, countries, new approaches, emerging trends, and future directions for sustainable development. This study is unique in that it provides a comprehensive, updated review of ZEBs and energy efficiency trends. Moreover, this study could help identify limitations for future policymakers, practitioners, and academics. The empirical section of this paper, through a case study, presents an example of a low-energy single-family building located in Poland.
Nonlinear Stiffness of Semi-Fixed Dowel Joints in Semi-Integral Bridges
Authors: Xu Zhen , Chen Baochun , Huang Fuyun , Zhuang Yizhou , Luo Xiaoye , Shi Feiting
Published: February 2022

The semi-fixed dowel joint studied in this paper consists of a steel dowel with rubber sleeves and a rubber pad to connect the superstructure and the substructure and to support the main beam. This type of joint is suitably used in retrofitting a jointed bridge with high-stiffness substructure into a semi-integral bridge. This study aimed to investigate the nonlinear stiffness of the joint, which is the key parameter in the retrofitting design. Taking the real joint in a retrofitted semi-integral bridge as a prototype, tests on full-scale models were carried out, in which the wall thickness of the rubber sleeve and the thickness of the rubber pad were taken as parameters. The test results show that the wall thickness of the rubber sleeve is the main factor affecting the radial stiffness and the flexural stiffness of the semi-fixed dowel joint. With the decrease of the wall thickness of the rubber sleeve, the radial stiffness decreases while the flexural stiffness increases. However, the thickness of the rubber pad has little influence on both the radial and flexural stiffness of the joint, and its main role is only vertical loading-bearing. If the longitudinal deformation of the main beam of a bridge under maximum temperature variation is 10 mm as the allowance value of engineering design, the wall thickness of the rubber sleeve for this joint should not be less than 40 mm. At last, on the basis of experimental research and theoretical analysis, the formulas of radial stiffness and flexural stiffness are fitted; it can be used as reference for practical engineering application, or for formulation of relevant specifications.
Privacy-Aware and Secure Decentralized Air Quality Monitoring
Authors: Mrissa Michael , Tošić Aleksandar , Hrovatin Niki , Aslam Sidra , Dávid Balázs , Hajdu László , Krész Miklós , Brodnik Andrej , Kavšek Branko
Published: February 2022

Indoor Air Quality monitoring is a major asset to improving quality of life and building management. Today, the evolution of embedded technologies allows the implementation of such monitoring on the edge of the network. However, several concerns need to be addressed related to data security and privacy, routing and sink placement optimization, protection from external monitoring, and distributed data mining. In this paper, we describe an integrated framework that features distributed storage, blockchain-based Role-based Access Control, onion routing, routing and sink placement optimization, and distributed data mining to answer these concerns. We describe the organization of our contribution and show its relevance with simulations and experiments over a set of use cases.