bluuebunny/crossref_metadata_2025 huggingface dataset:
All the Pups We Cannot See: Cannibalism Masks Perinatal Death in Laboratory Mouse Breeding but Infanticide Is Rare
Authors: Brajon Sophie , Morello Gabriela Munhoz , Capas-Peneda Sara , Hultgren Jan , Gilbert Colin , Olsson Anna
Published: August 2021

Perinatal mortality is a major issue in laboratory mouse breeding. We compared a counting method using daily checks (DAILY_CHECK) with a method combining daily checks with detailed video analyses to detect cannibalisms (VIDEO_TRACK) for estimating the number of C57BL/6 pups that were born, that died and that were weaned in 193 litters from trios with (TRIO-OVERLAP) or without (TRIO-NO_OVERLAP) the presence of another litter. Linear mixed models were used at litter level. To understand whether cannibalism was associated with active killing (infanticide), we analysed VIDEO_TRACK recordings of 109 litters from TRIO-OVERLAP, TRIO-NO_OVERLAP or SOLO (single dams). We used Kaplan-Meier method and logistic regression at pup level. For DAILY_CHECK, the mean litter size was 35% smaller than for VIDEO_TRACK (p < 0.0001) and the number of dead pups was twice lower (p < 0.0001). The risk of pup loss was higher for TRIO-OVERLAP than TRIO-NO_OVERLAP (p < 0.0001). A high number of pup losses occurred between birth and the first cage check. Analyses of VIDEO_TRACK data indicated that pups were clearly dead at the start of most of the cannibalism events and infanticide was rare. As most pups die and disappear before the first cage check, many breeding facilities are likely to be unaware of their real rates of mouse pup mortality.
The Association between Selected Dietary Minerals and Mastitis in Dairy Cows—A Review
Authors: Libera Kacper , Konieczny Kacper , Witkowska Katarzyna , Żurek Katarzyna , Szumacher-Strabel Małgorzata , Cieslak Adam , Smulski Sebastian
Published: August 2021

The aim of this paper is to describe the association between selected dietary minerals and mastitis in dairy cows. Minerals are a group of nutrients with a proven effect on production and reproductive performance. They also strongly affect immune system function. In particular their deficiencies may result in immunosuppression, which is a predisposing factor for udder inflammation occurrence. The role of selected dietary minerals (including calcium, phosphorus, magnesium, selenium, copper and zinc) has been reviewed. Generally, minerals form structural parts of the body; as cofactors of various enzymes they are involved in nerve signaling, muscle contraction and proper keratosis. Their deficiencies lead to reduced activity of immune cells or malfunction of teat innate defense mechanisms, which in turn promote the development of mastitis. Special attention was also paid to minerals applied as nanoparticles, which in the future may turn out to be an effective tool against animal diseases, including mastitis. To conclude, minerals are an important group of nutrients, which should be taken into account on dairy farms when aiming to achieve high udder health status.
Calcinosis in Alpaca Crias (Vicugna pacos) Due to Vitamin D Intoxication—Clinical, Laboratory and Pathological Findings with a Focus on Kidney Function
Authors: Wagener Matthias Gerhard , Helmer Carina , Kammeyer Patricia , Kleinschmidt Sven , Punsmann Teresa Maria , Meilwes Johanna Maria , Schwennen Cornelia , von Altrock Alexandra , Wilkens Mirja , Schwert Barbara , von Keyserlingk-Eberius Nicole , Ganter Martin
Published: August 2021

Alpacas kept in Central Europe are often deficient in vitamin D3, which is supplemented orally or by injection by the owners or veterinarians. Vitamin D3 can be specified in two different units (IU and µg), which differ by a factor of 40. By mixing up these units, an overdosage can be induced. In this study, three alpaca crias were examined after vitamin D3 intoxication, with particular reference to kidney function. All three animals developed non-specific clinical alterations 1–2 weeks after a vitamin D3 overdose of approximately 40 times. Plasma of the animals revealed several alterations. The main findings were severe azotemia, hypercalcemia and hyperphosphatemia, 15 days after treatment. Kidney function analysis (endogenous creatinine clearance) in two of the crias revealed severe glomerular damage. All crias died despite intensive treatment within 23 days after vitamin D3 treatment. Necropsy revealed calcification in different organs, mainly the kidneys, lungs and liver. Since nine other crias in the same group were treated with comparable doses of vitamin D3 and no clinical signs were observed in these animals, it is concluded that individual animals show different levels of sensitivity to vitamin D3.
A Survey of Horse Selection, Longevity, and Retirement in Equine-Assisted Services in the United States
Authors: Rankins Ellen M. , Wickens Carissa L. , McKeever Kenneth H. , Malinowski Karyn
Published: August 2021

Little published information exists on the horses in equine-assisted services (EAS), particularly their selection, longevity, and retirement. The purpose of this study was to characterize horses and procedures used in EAS. A pilot survey was developed using focus group discussions and distributed to Professional Association of Therapeutic Horsemanship, International (PATH Intl) centers in Florida (n = 45, Part I) before further modification and distribution to members of PATH Intl., American Hippotherapy Association (AHA), eagala, and Certified Horsemanship Association (CHA) (n = 26,000, Part II). Response rates were 36% (Part I) and 0.7% (Part II). Centers report a median of 10 (Part I) or 9 (Part II) horses and ponies. Selection procedures included initial screening (Part I = 100%, Part II = 96%), pre-purchase or pre-donation exam (I = 64%, II = 60%), acclimation period (I = 100%, II = 84%), trial period (I = 91%, II = 90%), and other (II = 11%). Horses remained active in programs for less than a year to over 20 years with the greatest number working 7–10 (Part I) or 1–6 (Part II) yr. In Part I of the study, behavior (44%) was the leading cause of retirement followed by unsoundness (33%). In Part II, unsoundness was the highest ranked response followed by behavior. Behavior, soundness, and health emerged as key factors in horse selection and retirement. Future work should focus on investigating these issues at an individual horse level.
Effects of Bait on Male White-Tailed Deer Resource Selection
Authors: Johnson James T. , Chandler Richard B. , Conner L. Mike , Cherry Michael J. , Killmaster Charlie H. , Johannsen Kristina L. , Miller Karl V.
Published: August 2021

Bait is often used to increase wildlife harvest susceptibility, enhance viewing opportunities, and survey wildlife populations. The effects of baiting depend on how bait influences space use and resource selection at multiple spatial scales. Although telemetry studies allow for inferences about resource selection within home ranges (third-order selection), they provide limited information about spatial variation in density, which is the result of second-order selection. Recent advances in spatial capture-recapture (SCR) techniques allow exploration of second- and third-order selection simultaneously using non-invasive methods such as camera traps. Our objectives were to describe how short-term baiting affects white-tailed deer (Odocoileus virginianus) behavior and distribution. We fit SCR models to camera data from baited and unbaited locations in southwestern Georgia to assess the effects of short-term baiting on second- and third-order selection of deer during summer and winter surveys. We found little evidence of second-order selection during late summer or early winter surveys when camera surveys using bait are typically conducted. However, we found evidence for third-order selection, indicating that resource selection within home ranges is affected. Concentrations in space use resulting from baiting may enhance disease transmission, change harvest susceptibility, and potentially bias the outcome of camera surveys using bait.
Assessment of Intestinal Immunity and Permeability of Broilers on Partial Replacement Diets of Two-Stage Fermented Soybean Meal by Bacillus velezensis and Lactobacillus brevis ATCC 367
Authors: Tsai Chia Fen , Lin Li Jen , Wang Chao Humg , Tsai Ching Sung , Chang Shang Chang , Lee Tzu Tai
Published: August 2021

The effect of soybean peptides from fermented soybean meal on the intestinal immunity and tight junction of broilers was assessed. Roughly, two-stage fermented soybean meal prepared with Bv and Lb (FSBMB+L), which has nearly three times higher soluble peptides than soybean meal (SBM), and reduced galacto-oligosaccharide (GOS) content and allergen protein. The one-stage fermented by Bv (FSBMB) has the highest soluble peptides, while commercial lactic acid bacteria (FSBML) has the highest Lactic acid bacteria count; these were used to compare the differences in the process. Ross308 broilers (n = 320) were divided into four groups: SBM diet and a diet replaced with 6% FSBMB+L, FSBMB, or FSBML. The growth performance was recorded during the experiment, and six birds (35-day-old) per group were euthanized. Analysis of their jejunum and ileum showed that the fermented soybean meal significantly improved the villus height in the jejunum (p < 0.05) and reduced the crypt hyperplasia. The FSBMB group had the highest reducing crypt depth; however, the FSBMB+L group had the highest villus height/crypt depth in the ileum (p < 0.05). In the jejunum, the relative mRNA of CLDN-1 and Occludin increased 2-fold in the treatments, and ZO-1 mRNA increased 1.5 times in FSBML and FSBMB+L (p < 0.05). Furthermore, the level of NF-κB and IL-6 mRNAs in FSBML increased, respectively, by 4 and 2.5 times. While FSBMB, along with FSBMB+L, had a 1.5-fold increase in the mRNA of IL-10, that of NF-κB increased 2-fold. FSBMB+L and FSBMB singly led to a 2- and 3-fold increase in IL-6 mRNA, respectively (p < 0.05). FSBMB and FSBMB+L can also upregulate MUC2 in the jejunum (p < 0.05). In short, using the soybean peptides from two-stage fermented soybean meal can ameliorate the negative factors of SBM and effectively regulate immune expression and intestinal repair, which will help broilers maintain intestinal integrity.
Effects of Differences in Fibre Composition and Maturity of Forage-Based Diets on the Microbial Ecosystem and Its Activity in Equine Caecum and Colon Digesta and Faeces
Authors: Muhonen Sara , Sadet-Bourgeteau Sophie , Julliand Véronique
Published: August 2021

Fibrous feeds are essential for horses. When developing feeding regimens promoting health and performance, we need to understand the digestion of plant cell walls and the functioning of the hindgut microbial ecosystem. Our objective was to investigate the effect of grass fibre maturity and legume forage on the hindgut microbiota and its activity. Six caecum and colon fistulated geldings were fed three diets differing in fibre composition: concentrate and late harvested grass haylage (35:65 energy ratio) (C); early and late harvested grass haylage (80:20) (G); lucerne and late harvested grass haylage (80:20) (L) for 28 days in a Latin-square design. No differences were measured in total bacteria concentrations, fungi and protozoa numbers nor in cellulolytic bacteria concentrations between the diets. Short-chain fatty acid concentrations did not differ between diets, but a lower (acetate + butyrate)/propionate ratio when the horses were fed Diet C, compared to G and L, was observed, suggesting lower fibrolytic and higher amylolytic activity. The pH increased when the horses were fed Diet L and decreased when fed C and G from caecum to faeces. The buffering capacity (BC) of hindgut digesta was five to fifteen-fold higher than that of the feeds, suggesting a decreased effect of feed BC as digesta travelled through the digestive tract. In conclusion, an early harvested forage opens up the possibility for forage-only diets, providing high energy without the negative effects of concentrate.
Assessment of Age Effects on Ovarian Hemodynamics Using Doppler Ultrasound and Progesterone Concentrations in Cycling Spanish Purebred Mares
Authors: Requena Francisco , Campos María Joana A. P. M. , Martínez Marín Andrés Luis , Camacho Rocío , Giráldez-Pérez Rosa M. , Agüera Estrella I.
Published: August 2021

In equine reproduction, accurate and timely detection of the moment of ovulation is of great importance. Power Doppler ultrasound technology is a non-invasive method that enables to assess the morpho-echogenic features and blood flow changes during the estral cycle in mares. The objective of the present study was to evaluate the influence of age on ultrasonographic parameters (follicular diameter, follicular blood flow—FBF, corpus luteum (CL) area and corpus luteum blood flow—CLBF) and blood plasma progesterone concentrations in cycling Spanish Purebred mares (15 less than 8 years old and 15 equal o higher than 8 years old). The ultrasound images obtained were analyzed with the Image Colour Summarizer software, which allows the quantification of the pixels of each image. Young mares had significantly higher FBF, CLBF and plasma progesterone levels. Moreover, linear regression analysis showed that blood progesterone levels could be predicted in both groups from CLBF with moderate precision and accuracy. In conclusion, Power Doppler was useful to assess ovarian hemodynamics. Our results support that age is a factor that significantly influences FBF and CLBF as well as blood progesterone concentration in mares. More studies would be needed to develop high precision and accuracy predictive models of blood progesterone concentration from CLBF measured by Power Doppler.
Polymorphisms of Codons 110, 146, 211 and 222 at the Goat PRNP Locus and Their Association with Scrapie in Greece
Authors: Gelasakis Athanasios I. , Boukouvala Evridiki , Babetsa Maria , Katharopoulos Efstathios , Palaska Vayia , Papakostaki Dimitra , Giadinis Nektarios D. , Loukovitis Dimitrios , Langeveld Jan P. M. , Ekateriniadou Loukia V.
Published: August 2021

Scrapie is considered an endemic disease in both sheep and goats in Greece. However, contrary to sheep, in goats more than one prion protein (PrP) polymorphism has been recognized as a candidate for resistance breeding against the disease. For an impression, candidates which are circulating, (i) brain samples (n = 525) from scrapie-affected (n = 282) and non-affected (n = 243) animals within the national surveillance program, and (ii) individual blood samples (n = 1708) from affected (n = 241) and non-affected (n = 1467) herds, in a large part of mainland Greece and its islands, were collected and assayed. A dedicated Taqman method was used to test for amino acid polymorphisms 110T/P, 146N/S/D, 211R/Q, and 222Q/K. Highly prevalent genotypes were 110TT, 146NN, 211RR, and 222QQ. The frequencies of polymorphisms in blood and negative brain samples for codons 110P, 211Q, and 222K were 4.0%, 3.0%, and 1.9%, respectively, while 146D (0.7%) was present only on Karpathos island. Codon 110P was exclusively found in scrapie-negative brains, and homozygous 110P/P in two scrapie-negative goats. It is concluded that breeding programs in Karpathos could focus on codon 146D, while in other regions carriers of the 110P and 222K allele should be sought. Case-control and challenge studies are now necessary to elucidate the most efficient breeding strategies.
Sustainable Management of Voluntary Culling Risk in Primiparous Zaraibi Goats in Egypt: Roles of Season and Reproductive and Milk Production-Related Traits
Authors: El-Raghi Ali Ali , Hassan Mahmoud A. E. , El-Ratel Ibrahim T. , Hashem Nesrein M. , Abdelnour Sameh A.
Published: August 2021

The purpose of the current study is to reconnoiter the relationships between season (birth season, BS, and kidding season, KS), reproductive traits (birth weight, BW; total litter size at birth, TLS; litter size at weaning, LSW; the number of kids dead, NKD, and; age at first kidding, AFK) and milk production (total milk yield, TMY; average daily milk yield, DMY, and; lactation period, LP), with voluntary culling risk in primiparous Zaraibi goats. Records of 637 primiparous does were collected during the period 2008–2014 from a herd of Zaraibi goats raised at the El-Serw Experimental Station, which belongs to the Animal Production Research Institute. Our data revealed that the voluntary and involuntary culling was 89.12% and 10.88%, respectively. Moreover, the BW, TLS, LSW, NKD, TMY, DMY, LP, AFK had significant effects on culling risk (p < 0.05), while both the season of birth and kidding did not exhibit significant effects on culling risk. The thinnest goats at birth were more likely to be culled compared to those with body energy reserves. Does with weaned twins and triplets kids were 9.5% (OR = 0.905) and 43% (OR = 0.570), respectively less likely to be culled compared to those with singles. Likewise, does with dead twins and triplets kids had 2.566 and 2.138 times, respectively higher odds of culling compared to those with singles. Interestingly, culling risk decreased with 74.6% (OR = 0.254), 79.8% (OR = 0.202), and 75.6% (OR = 0.244) in does with TMY, LP, and DMY more than 230 kg, 260 days, and 0.800 kg, respectively compared to their counterparts (less than 185 kg, 240 day, and 0.500 kg, respectively). Moreover, culling risk increased steadily along with increasing AFK; the animals with an AFK of more than 24 months had 2.974 times higher odds of culling compared to those with an AFK that varied between 22 and 24 months. It could be concluded that the most critical issues for higher culling probability in dairy goats were attributed to the lower TMY (<185 kg) and DMY (<0.5 kg), and shorter LP (<245 days), as well as older age at first kidding (>2 years). This putative information could be used as indicators to enhance the management and genetic approaches in dairy goats and thus sustain productivity with low cost.
Computational Fluid Dynamics Analysis of Alternative Ventilation Schemes in Cage-Free Poultry Housing
Authors: Chen Long , Fabian-Wheeler Eileen E. , Cimbala John M. , Hofstetter Dan , Patterson Paul
Published: August 2021

This work investigated alternative ventilation schemes to help define a proper ventilation system design in cage-free hen houses with the goal of assuring bird welfare through comfortable conditions. Computational fluid dynamics (CFD) modeling was employed to simulate indoor and outdoor airflows to quantify the effectiveness of ventilation systems in maintaining suitable and uniform living conditions at the hen level. Four three-dimensional CFD models were developed based on a full-scale floor-raised layer house, corresponding to ventilation schemes of the standard top-wall inlet, sidewall exhaust, and three alternatives: mid-wall inlet, ceiling exhaust; mid-wall inlet, ridge exhaust; and mid-wall inlet, attic exhaust with potential for pre-treatment of exhaust air. In a sophisticated and powerful achievement of the analysis, 2365 birds were individually modeled with simplified bird-shapes to represent a realistic number, body heat, and airflow obstruction of hens housed. The simulated ventilation rate for the layer house models was 1.9–2.0 m3/s (4100 ft3/min) in the desired range for cold weather (0 °C). Simulation results and subsequent analyses demonstrated that these alternative models had the capacity to create satisfactory comfortable temperature and air velocity at the hen level. A full-scale CFD model with individual hen models presented robustness in evaluating bird welfare conditions.
Pharmacokinetic Behaviour of Enrofloxacin after Single Intramuscular Dosage in American Black Vultures (Coragyps atratus)
Authors: Waxman Samanta , de Lucas José Julio , Wiemeyer Guillermo , Torres Bianchini Laura , San Andrés Manuel Ignacio , Rodríguez Casilda
Published: August 2021

The aim of the study was to investigate the intramuscular pharmacokinetics of enrofloxacin in black vultures (Coragyps atratus). The pharmacokinetics of a single intramuscular dose (10 mg/kg) of enrofloxacin was studied in six vultures. Plasma concentrations of enrofloxacin and its active metabolite, ciprofloxacin, were determined by high-performance liquid chromatography (HPLCuv). Pharmacokinetic parameters were estimated using non-compartmental and compartmental analysis. After intramuscular administration, enrofloxacin showed a rapid and complete absorption, reaching a Cmax value of 3.26 ± 0.23 μg/mL at 1.75 ± 0.53 h. A long terminal half-life of 19.58 h has been observed. Using previously published MIC values to perform a PK/PD analysis, cumulative fraction responses obtained after Monte Carlo simulation for AUC/MIC > 30, 50 and 125 were 72.93%, 72.34% and 30.86% for E. coli and 89.29%, 88.89% and 58.57% for Mycoplasma synoviae, respectively. Cumulative fraction responses obtained for Cmax/MIC index were 33.93% and 40.18% for E. coli and M. synoviae, respectively. The intramuscular administration of 10 mg/kg could be appropriate to treat infectious diseases caused by gram-positive bacteria with MIC value lower than 1 µg/mL; however, although enrofloxacin showed a slow elimination in black vultures, plasma concentrations were insufficient to reach the gram-negative stablished breakpoints.
Terpenoids and Polyphenols as Natural Antioxidant Agents in Food Preservation
Authors: Gutiérrez-del-Río Ignacio , López-Ibáñez Sara , Magadán-Corpas Patricia , Fernández-Calleja Luis , Pérez-Valero Álvaro , Tuñón-Granda Mateo , Miguélez Elisa M. , Villar Claudio J. , Lombó Felipe
Published: August 2021

Synthetic antioxidant food additives, such as BHA, BHT and TBHQ, are going through a difficult time, since these products generate a negative perception in consumers. This has generated an increased pressure on food manufacturers to search for safer natural alternatives like phytochemicals (such as polyphenols, including flavonoids, and essential oils rich in terpenoids, including carotenoids). These plant bioactive compounds have antioxidant activities widely proven in in vitro tests and in diverse food matrices (meat, fish, oil and vegetables). As tons of food are wasted every year due to aesthetic reasons (lipid oxidation) and premature damage caused by inappropriate packaging, there is an urgent need for natural antioxidants capable of replacing the synthetic ones to meet consumer demands. This review summarizes industrially interesting antioxidant bioactivities associated with terpenoids and polyphenols with respect to the prevention of lipid oxidation in high fat containing foods, such as meat (rich in saturated fat), fish (rich in polyunsaturated fat), oil and vegetable products, while avoiding the generation of rancid flavors and negative visual deterioration (such as color changes due to oxidized lipids). Terpenoids (like monoterpenes and carotenoids) and polyphenols (like quercetin and other flavonoids) are important phytochemicals with a broad range of antioxidant effects. These phytochemicals are widely distributed in fruits and vegetables, including agricultural waste, and are remarkably useful in food preservation, as they show bioactivity as plant antioxidants, able to scavenge reactive oxygen and nitrogen species, such as superoxide, hydroxyl or peroxyl radicals in meat and other products, contributing to the prevention of lipid oxidation processes in food matrices.
Machining the Surface of Orthopedic Stent Wire Using a Non-Toxic Abrasive Compound in a Magnetic Abrasive Finishing Process
Authors: Kim Jeong Su , Heng Lida , Chanchamnan Sieb , Mun Sang Don
Published: August 2021

The orthopedic stent wire is one of the critical medical components, which is mainly used for the replacement of physically damaged parts in the human body. Therefore, a smooth surface and lack of toxic substances on the surface of this component are highly demanded. In this study, a magnetic abrasive finishing (MAF) process was carried out using a non-toxic abrasive compound (a mixture of iron powder, diamond particles, cold cream, and eco-friendly oils) to achieve high-quality surface finishing of orthopedic stent wire. The surface roughness (Ra) of the stent wire was investigated according to various processing parameters: different rotational speeds (500, 1000, and 2000 rpm), diamond particle sizes (1.0 µm), and three eco-friendly oils (olive oil: C98H184O10; grapeseed oil: C18H32O2; and castor oil: C57H104O9) within 300 s of the finishing time. The results showed that the surface roughness of the wire was reduced to 0.04 µm with a rotation speed of 1000 rpm and a diamond particle size of 1 µm when using grapeseed oil. SEM microimages and EDS analysis showed that the MAF process using a non-toxic abrasive compound could improve the surface quality of orthopedic Ni-Ti stent wire with a lack of toxic substances on the surface finish.
A Novel Acceleration-Based Moving Force Identification Algorithm to Detect Global Bridge Damage
Authors: Wang Shuo , OBrien Eugene J. , McCrum Daniel P.
Published: August 2021

This paper presents a new moving force identification (MFI) algorithm that uses measured accelerations to infer applied vehicle forces on bridges. Previous MFI algorithms use strain or deflection measurements. Statistics of the inferred forces are used in turn as indicators of global bridge damage. The new acceleration-based MFI algorithm (A-MFI) is validated through numerical simulations with a coupled vehicle-bridge dynamic interaction model programmed in MATLAB. A focussed sensitivity study suggests that results are sensitive to the accuracy of the vehicle velocity data. The inferred Gross Vehicle Weight (GVW), calculated by A-MFI, is proposed as the bridge damage indicator. A real weigh-in-motion database is used with a simulation of vehicle/bridge interaction, to validate the concept. Results show that the standard deviation of inferred GVWs has a good correlation with the global bridge damage level.
Optimization of Composition and Technological Factors for the Lightweight Fiber-Reinforced Concrete Production on a Combined Aggregate with an Increased Coefficient of Structural Quality
Authors: Mailyan Levon R. , Beskopylny Alexey N. , Meskhi Besarion , Stel’makh Sergey A. , Shcherban Evgenii M. , Ananova Oxana
Published: August 2021

In recent years, developing lightweight concrete with both the necessary and sufficient strength characteristics is essential in the construction industry. This article studies the influence of the volumetric composition of lightweight fiber-reinforced concrete (LFRC) and the method of its distribution during the preparation of the fiber–concrete mixture on the strength and deformation characteristics of LFRC on a combined aggregate. The optimal grain size of the porous filler was calculated by the mathematical planning method of the experiment. Regression models of the strength and deformation characteristics on the volumetric content of fiber and its distribution method were obtained. The most effective combination of these factors has been determined. The model shows that the increase in compressive strength was 12%, the value of the prismatic strength increased by 25%, the bending tensile strength increased by 34%, and the axial tensile strength increased by 11%. The ultimate strains during axial compression decreased by 10%, axial tension decreased by 12%, and the elasticity modulus increased by 11% compared to the test results of the control composition samples without fiber and pumice. The coefficient of constructive quality (CCQ) of the LFRC on a combined aggregate compared to concrete with the control composition without fiber and pumice showed an increase of more than 32%. It was also found that fiber reinforcement with basalt fibers with a combination of heavy and porous aggregates achieves a synergistic effect together.
Pareto Effect of LMI for Ship Propulsion
Authors: Rybczak Monika , Podgórski Kamil
Published: August 2021

The aim of this study was to analyze the dynamics of a multidimensional object based on the Pareto curve for the Linear Matrix Inequalities (LMI) controller. The study was carried out based on an available “Blue Lady” training vessel model controller with the use of a MATLAB and Simulink simulation package. Research was focused on optimising both the energy to be used when manoeuvring and the ship’s dynamics. Analysis was combined with the application of H2/H∞ norms finding the Pareto optimal solution for mixed norms used at the γ∞ parameter. Observations for a multidimensional ship model proved that it is possible to optimize the system, using principles of the Pareto curve, to reduce energy consumption in steering-propulsion systems while performing precise manoeuvres in ports correctly. Parameter values, received from observations of operation of individual steering-propulsion systems, proved to be reasonable.
Application of Conventional and Non-Conventional Extraction Methods to Obtain Functional Ingredients from Jackfruit (Artocarpus heterophyllus Lam.) Tissues and By-Products
Authors: Cruz-Casillas Frida Camila , García-Cayuela Tomás , Rodriguez-Martinez Veronica
Published: August 2021

In recent years, researchers in the pharmaceutical and food areas focused on finding the best ways to take advantage of functional ingredients present in jackfruit tissues and by-products such as phenolics and pectin. Many of these studies focused on adding value to the by-products and decreasing their negative environmental impact. However, the type, quantity, and characteristics of jackfruit functional ingredients are highly dependent on the extraction method, either through conventional or non-conventional technologies, and the jackfruit tissue used, with peel and seeds being the most studied. The reported studies suggest that extractions and pre-treatments with emerging technologies such as ultrasounds, microwaves, radio frequency, or supercritical fluids can facilitate the release of functional ingredients of jackfruit; reduce the time and energy consumption required; and, in some cases, improve extraction yields. Therefore, emerging technologies could increase the functional potential of jackfruit and its by-products, with promising applications in the pharmaceutical and nutraceutical industries.
Intersection-Based Routing with Fuzzy Multi-Factor Decision for VANETs
Authors: Cao Zhenbo , Fan Zujie , Kim Jaesool
Published: August 2021

Due to the limitations of the urban environment, the data transferred between vehicles can only change direction at the intersections. Therefore, the routing decision at an intersection will largely affect the overall routing decision. In this article, we propose an Intersection-Based Routing with Fuzzy Multi-Factor Decision (IRFMF), which utilizes several factors to decide the next road segment. In the scheme, each intersection introduces three factors including the direction, the number of lanes, and the traffic. After the fuzzification and defuzzification of these factors, the candidate segment with the highest evaluation will be selected. The simulation shows a significant improvement of VANETs performance on packet delivery ratio and end-to-end delay.
Assessing and Modelling the Interactions of Instrumented Particles with Bed Surface at Low Transport Conditions
Authors: Alhusban Zaid , Valyrakis Manousos
Published: August 2021

Sediment transport at near threshold to low transport stages (below the continuous transport) can still be affected by flow turbulence and its dynamics can benefit from further comprehensive studies. This study uses an instrumented particle embedded with micro electromechanical sensors (MEMS) to allow tracking the motions and forces acting on it, leading to and during its transport. Instrumented particle transport experiments were carried out at laboratory flume under a range of flow conditions. The probability distributions functions (PDFs) of bed load particle instantaneous velocities, hop distances and associated travel times (measured from start to stop of transport) were obtained for all the performed experiments with varying flow rates and particle density. The modelled distributions are useful and enable a deeper understanding of bed load sediment transport dynamics from a Lagrangian perspective. Furthermore, the results analyzed from the instrumented particle (including the particle’s transport mode) were validated using visual particle tracking methods (top and side cameras). The findings of this study demonstrate that for the range of turbulent flows trialed herein, the instrumented particle can be a useful, accessible, and low-cost tool for obtaining particle transport dynamics, having demonstrated satisfactory potential for field deployment in the near future.
Conducting an International, Exploratory Survey to Collect Data on Honey Bee Disease Management and Control
Authors: Mezher Ziad , Bubnic Jernej , Condoleo Roberto , Jannoni-Sebastianini Filippo , Leto Andrea , Proscia Francesco , Formato Giovanni
Published: August 2021

The Istituto Zooprofilattico Sperimentale del Lazio e della Toscana (IZSLT) and the International Federation of Beekeepers’ Associations (Apimondia) set up a worldwide survey in 2015–2017 to gather information on beekeepers’ perceptions concerning good beekeeping practices, the main honey bee diseases, and the technical assistance they receive. The on-line dissemination of the survey was facilitated by the “Technologies and Practices for Small Agricultural Producers” platform (TECA) of the Food and Agriculture Organization of the United Nations (FAO). In total, 248 questionnaires were received from the European region and the results are reported here. Varroosis remains the biggest concern and is the most important reason for beekeepers’ use of veterinary medicines. However, a sustainable approach to managing Varroa was detected. American foulbrood (AFB) and European foulbrood (EFB) infections are mainly managed through the shook swarm technique or burning the hives. Concerning technical assistance for disease management, beekeepers were mainly supported by their associations or expert beekeepers. Relevant data were collected and analyzed but information from many low-income countries in Africa or Asia is still missing, and more efforts are needed to fill the knowledge gaps.
Stability Analysis of Soil Flow Protector and Design Method for Estimating Optimal Length
Authors: Son Suwon , Choi Moonbong , Yoo Jaewon
Published: August 2021

Underground cavities can develop below structures, leading to ground settlement and hindering the development of urban infrastructure. Soil flow protectors (SFPs) have been developed to prevent and alleviate problems due to the formation of such cavities. In this study, we performed scaled model experiments to develop a design method for an SFP with an adequate safety factor under different installation lengths of its upper and lower parts in sandy ground. The installation of the SFP reduced the average surface settlement ratio to the range of 0.44–0.72, thus demonstrating its effectiveness in reducing ground settlement. In addition, we proposed a relational equation for determining the optimal length ratio of the SFP and the settlement ratio. An analysis of the influencing factors showed that the lower part of the SFP influenced the settlement reduction, whereas the upper part influenced the stability of the SFP depending on the ground settlement ratio. Finally, we have proposed an optimal length equation for the SFP and presented a flowchart for the design method. The results of this study can serve as a design basis for the efficient construction of infrastructure.
Association between Serum Heat Shock Proteins and Gamma-Delta T Cells—An Outdated Clue or a New Direction in Searching for an Anticancer Strategy? A Short Report
Authors: Pawlik-Gwozdecka Dorota , Sakowska Justyna , Zieliński Maciej , Górska-Ponikowska Magdalena , Cappello Francesco , Trzonkowski Piotr , Niedźwiecki Maciej
Published: August 2021

HSPs demonstrate a strong association with gamma-delta (γδ) T cells. Most of the studies regarding interactions between the parameters were conducted in the 1990s. Despite promising results, the concept of targeting γδ T cells by HSPs seems to be a forgotten direction due to potent non-peptidic phosphoantigens rather than HSPs have been found to be the essential stimulatory components for human γδ cells. Currently, with greater knowledge of lymphocyte diversity, and more accurate diagnostic methods, we decided to study the correlation once again in the neoplastic condition. Twenty-one children with newly diagnosed acute lymphoblastic leukaemia (ALL) were enrolled on the study. Serum HSP90 concentrations were evaluated by an enzyme-linked immunosorbent assay (ELISA), subsets of γδ T cells (CD3+ γδ, CD3+ γδ HLA/DR+, CD4+ γδ and CD8+ γδ) by flow cytometry. We have shown statistically relevant correlations between serum HSP90 and CD3+ HLA/DR+ γδ T cells in paediatric ALL at diagnosis (R = 0.53, p < 0.05), but not after induction chemotherapy. We also have demonstrated decreased levels of both serum HSP90 and CD3+ HLA/DR+ γδ T cells before treatment, which may indirectly indicate dose-dependent unknown interaction between the parameters. The results of our study may be a good introduction to research on the association between HSPs and CD3+ HLA/DR+ γδ T cells, which could be an interesting direction for the development of anti-cancer strategies, not just for childhood ALL.
The UAE Cloud Seeding Program: A Statistical and Physical Evaluation
Authors: Al Hosari Taha , Al Mandous Abdulla , Wehbe Youssef , Shalaby Abdeltawab , Al Shamsi Noor , Al Naqbi Hajer , Al Yazeedi Omar , Al Mazroui Alya , Farrah Sufian
Published: August 2021

Operational cloud seeding programs have been increasingly deployed in several countries to augment natural rainfall amounts, particularly over water-scarce and arid regions. However, evaluating operational programs by quantifying seeding impacts remains a challenging task subject to complex uncertainties. In this study, we investigate seeding impacts using both long-term rain gauge records and event-based weather radar retrievals within the framework of the United Arab Emirates (UAE) National Center of Meteorology’s operational cloud seeding program. First, seasonal rain gauge records are inter-compared between unseeded (1981–2002) and seeded (2003–2019) periods, after which a posteriori target/control regression is developed to decouple natural and seeded rainfall time series. Next, trend analyses and change point detection are carried out over the July-October seeding periods using the modified Mann-Kendall (mMK) test and the Cumulative Sum (CUSUM) method, respectively. Results indicate an average increase of 23% in annual surface rainfall over the seeded target area, along with statistically significant change points detected during 2011 with decreasing/increasing rainfall trends for pre-/post-change point periods, respectively. Alternatively, rain gauge records over the control (non-seeded) area show non-significant change points. In line with the gauge-based statistical findings, a physical analysis using an archive of seeded (65) and unseeded (87) storms shows enhancements in radar-based storm properties within 15–25 min of seeding. The largest increases are recorded in storm volume (159%), area cover (72%), and lifetime (65%). The work provides new insights for assessing long-term seeding impacts and has significant implications for policy- and decision-making related to cloud seeding research and operational programs in arid regions.
The Impact of Smog Pollution on Audit Quality: Evidence from China
Authors: Li Bin , Zhou Ying , Zhang Tingyu , Liu Yang
Published: August 2021

Audit quality usually refers to the quality that the auditing services accounting firm auditors provide to an enterprise in the form of an audit report. This study empirically analyzes the impact mechanism of smog pollution on audit quality, based on the data of A-share listed companies in the Shanghai and Shenzhen Stock Exchanges during the period 2013 to 2017 and the air quality monitoring data released by the China National Environmental Monitoring Centre covering the period 2013 to 2018. First, the empirical results show that smog pollution can lead to a decline in audit quality. Second, audit time plays a partial mediating role in the relationship between smog pollution and audit quality. Further analysis indicates that the negative impact of smog pollution on audit quality and the intermediary role played by audit time are only significant in the sample of “top 10” accounting firms. Third, the enterprise′s internal control level positively moderates the mediating effect of audit time on smog pollution and audit quality.
Radon Awareness and Policy Perspectives on Testing and Mitigation
Authors: Novilla M. Lelinneth B. , Johnston James D. , Beard John D. , Pettit Lucas L. , Davis Siena F. , Johnson Claire E.
Published: August 2021

One in three homes in Utah (USA) contains dangerous levels of radon. Except for a radon mitigation certification law, Utah’s radon laws are nonexistent. To determine public perception of state policies on radon testing and mitigation, a social cognitive theory-based 52-item questionnaire was administered to residents (N = 307) who visited the Utah County Health Department (UCHD) during the study period. Respondents were divided into an Environmental Health Group (n = 110), who purchased a radon kit, and Vital Records Control Group (n = 197), who filed/obtained birth/death certificates at UCHD. Ninety percent responded they had never tested their homes for radon, and 99% were not aware of state policies regarding radon. Support for various radon policies was significantly associated with older age (odds ratios (OR): 0.37–0.52), being female (OR: 2.60–7.79), lower annual family income (OR: 2.27), and theoretical constructs of behavioral modeling (OR: 2.31–2.55) and risk perception (OR: 2.55–3.71). To increase awareness, testing, and remediation, respondents suggested increasing public education/awareness, requiring testing in homes, businesses, and public buildings, and increasing access to testing. Multi-sectoral radon risk reduction programs could incorporate behavioral modeling and risk perception as components to create a radon testing and mitigation culture in Utah.
Layered Iron Vanadate as a High-Capacity Cathode Material for Nonaqueous Calcium-Ion Batteries
Authors: Chae Munseok S. , Setiawan Dedy , Kim Hyojeong J. , Hong Seung-Tae
Published: August 2021

Calcium-ion batteries represent a promising alternative to the current lithium-ion batteries. Nevertheless, calcium-ion intercalating materials in nonaqueous electrolytes are scarce, probably due to the difficulties in finding suitable host materials. Considering that research into calcium-ion batteries is in its infancy, discovering and characterizing new host materials would be critical to further development. Here, we demonstrate FeV3O9∙1.2H2O as a high-performance calcium-ion battery cathode material that delivers a reversible discharge capacity of 303 mAh g−1 with a good cycling stability and an average discharge voltage of ~2.6 V (vs. Ca/Ca2+). The material was synthesized via a facile co-precipitation method. Its reversible capacity is the highest among calcium-ion battery materials, and it is the first example of a material with a capacity much larger than that of conventional lithium-ion battery cathode materials. Bulk intercalation of calcium into the host lattice contributed predominantly to the total capacity at a lower rate, but became comparable to that due to surface adsorption at a higher rate. This stimulating discovery will lead to the development of new strategies for obtaining high energy density calcium-ion batteries.
Impact of FasL Stimulation on Sclerostin Expression and Osteogenic Profile in IDG-SW3 Osteocytes
Authors: Kratochvilova Adela , Ramesova Alice , Vesela Barbora , Svandova Eva , Lesot Herve , Gruber Reinhard , Matalova Eva
Published: August 2021

The Fas ligand (FasL) is known from programmed cell death, the immune system, and recently also from bone homeostasis. As such, Fas signalling is a potential target of anti-osteoporotic treatment based on the induction of osteoclastic cell death. Less attention has been paid to osteocytes, although they represent the majority of cells within the mature bone and are the key regulators. To determine the impact of FasL stimulation on osteocytes, differentiated IDG-SW3 cells were challenged by FasL, and their osteogenic expression profiles were evaluated by a pre-designed PCR array. Notably, the most downregulated gene was the one for sclerostin, which is the major marker of osteocytes and a negative regulator of bone formation. FasL stimulation also led to significant changes (over 10-fold) in the expression of other osteogenic markers: Gdf10, Gli1, Ihh, Mmp10, and Phex. To determine whether these alterations involved caspase-dependent or caspase-independent mechanisms, the IDG-SW3 cells were stimulated by FasL with and without a caspase inhibitor: Q-VD-OPh. The alterations were also detected in the samples treated by FasL along with Q-VD-OPh, pointing to the caspase-independent impact of FasL stimulation. These results contribute to an understanding of the recently emerging pleiotropic effects of Fas/FasL signalling and specify its functions in bone cells.
The Effect of Oxygen and Micronutrient Composition of Cell Growth Media on Cancer Cell Bioenergetics and Mitochondrial Networks
Authors: Moradi Fereshteh , Moffatt Christopher , Stuart Jeffrey A.
Published: August 2021

Cancer cell culture is routinely performed under superphysiologic O2 levels and in media such as Dulbecco’s Modified Eagle Medium (DMEM) with nutrient composition dissimilar to mammalian extracellular fluid. Recently developed cell culture media (e.g., Plasmax, Human Plasma-Like Medium (HPLM)), which are modeled on the metabolite composition of human blood plasma, have been shown to shift key cellular activities in several cancer cell lines. Similar effects have been reported with respect to O2 levels in cell culture. Given these observations, we investigated how media composition and O2 levels affect cellular energy metabolism and mitochondria network structure in MCF7, SaOS2, LNCaP, and Huh7 cells. Cells were cultured in physiologic (5%) or standard (18%) O2 levels, and in physiologic (Plasmax) or standard cell culture media (DMEM). We show that both O2 levels and media composition significantly affect mitochondrial abundance and network structure, concomitantly with changes in cellular bioenergetics. Extracellular acidification rate (ECAR), a proxy for glycolytic activity, was generally higher in cells cultured in DMEM while oxygen consumption rates (OCR) were lower. This effect of media on energy metabolism is an important consideration for the study of cancer drugs that target aspects of energy metabolism, including lactate dehydrogenase activity.