bluuebunny/crossref_metadata_2025 huggingface dataset:
Düşünme Becerileri: Kritik Düşünme ve Öğretimi
Authors: PAYAM Mehmet Murat
Published: March 2022

Bilgi toplumunda, öğrencilerin öğrenme sürecine etkin olarak katılan, sahih kaynaklardan öğrenen ve öğrendiklerini hayatında tatbik eden bireyler olarak yetiştirilmeleri gerekmektedir. Eğitim sistemimizin ise ezbere dayandığı ve sadece bilgiyi aktardığı, mantıklı ve kritik düşünen bireyler yetiştirmekte yetersiz kaldığı sıkça dile getirilir. Bununla birlikte, günümüzde eğitim etkinliklerinden beklenen, düşünmeyi bilen, öğretme-öğrenme sürecinde bilgi edinmek için aktif bir biçimde rol üstlenen, kendi öğrenmesinin sorumluluğunu taşıyan, düşünme becerileriyle donanmış, kendine ve topluma faydalı ve üretici bireyler yetiştirmesidir. Yapılan deneysel çalışmalar da, düşüncenin eğitim yoluyla geliştirilebileceğini ve kişinin kritik düşünme becerileri kazanabileceğini göstermektedir. Kritik düşünme yoluyla akla kapı açılmalı ve fakat irade elden alınmamalıdır. Dolayısıyla, düşünme becerilerinin özellikle de kritik düşünmenin öğretimiyle sadece dilde kalan düşünce ve söylem tarzından uzak, derinlikli bir düşünce bütünlüğü kazanılacak, bilgilerin dışarıdan olduğu gibi alınıp depolanması yerine, yeni bilgilerin anlamlandırılarak oluşturulmasına ve onunla yaşanılmasına zemin hazırlanacaktır. Böylece kritik düşünmeyi öğrenme neticesinde, gelişmeleri doğru değerlendirme ve anlama yeteneği geliştikçe, iyi insanların dünya politikalarında daha aktif ve belirleyici rol almaları ve iyiliği ön plana çıkarma fırsatları doğacaktır. Zaten bugünkü şartlarda iyi insana düşen en büyük vazife de, sahih kaynaklardan kemaliyle doğru bilgileri öğrenmek, öğretmek ve kemaliyle tatbik edip tatbik ettirmektir. Bu bağlamda bu bildiride, düşünme becerileri, kritik düşünme ve okullarda kritik düşünme öğretimi üzerinde durulmuştur.
Optimization and Validation of a Custom-Designed Perfusion Bioreactor for Bone Tissue Engineering: Flow Assessment and Optimal Culture Environmental Conditions
Authors: Yamada Shuntaro , Yassin Mohammed A. , Schwarz Thomas , Mustafa Kamal , Hansmann Jan
Published: March 2022

Various perfusion bioreactor systems have been designed to improve cell culture with three-dimensional porous scaffolds, and there is some evidence that fluid force improves the osteogenic commitment of the progenitors. However, because of the unique design concept and operational configuration of each study, the experimental setups of perfusion bioreactor systems are not always compatible with other systems. To reconcile results from different systems, the thorough optimization and validation of experimental configuration are required in each system. In this study, optimal experimental conditions for a perfusion bioreactor were explored in three steps. First, an in silico modeling was performed using a scaffold geometry obtained by microCT and an expedient geometry parameterized with porosity and permeability to assess the accuracy of calculated fluid shear stress and computational time. Then, environmental factors for cell culture were optimized, including the volume of the medium, bubble suppression, and medium evaporation. Further, by combining the findings, it was possible to determine the optimal flow rate at which cell growth was supported while osteogenic differentiation was triggered. Here, we demonstrated that fluid shear stress up to 15 mPa was sufficient to induce osteogenesis, but cell growth was severely impacted by the volume of perfused medium, the presence of air bubbles, and medium evaporation, all of which are common concerns in perfusion bioreactor systems. This study emphasizes the necessity of optimization of experimental variables, which may often be underreported or overlooked, and indicates steps which can be taken to address issues common to perfusion bioreactors for bone tissue engineering.
Isolation of a Monoclonal Antibody and its Derived Immunosensor for Rapid and Sensitive Detection of 17β-Estradiol
Authors: Liang Jingru , Dong Hang , Xu Fei , Li Baowei , Li Haimei , Chen Limei , Li Mei , Liu Yingchu , Jiang Guosheng , Dong Jinhua
Published: March 2022

Estrogens are effective for stimulating several functions in living organisms and for regulating cancer development by promoting cell proliferation. Estradiol can disrupt the reproductive and endocrine systems, leading to the development of various diseases. In this study, the monoclonal antibody ESC9 was developed by immunizing mice with a 17β-estradiol (E2) conjugate, preparing an antibody phage display library, and screening monoclonal antibodies from the prepared library. An antibody with the same sequence as that of ESC9 has not been reported previously. The equilibrium dissociation constant between ESC9 and E2 was found to be 43.3 nM. Additionally, we generated an ESC9-derived immunosensor named as the ESC9 Quenchbody (Q-body), which can rapidly and sensitively detect E2. The assay can be completed within 2 min with a limit of detection of 3.9 pg/ml and half-maximal effective concentration of 154.0 ng/ml. Serum E2 levels were measured using the ESC9 Q-body without pretreatment with serum and with a high recovery rate of 83.3–126.7%. The Q-body immunosensor shows potential for clinical applications based on its excellent detection speed and sensitivity.
The Biosynthesis of D-1,2,4-Butanetriol From d-Arabinose With an Engineered Escherichia coli
Authors: Wang Jing , Chen Qiaoyu , Wang Xin , Chen Kequan , Ouyang Pingkai
Published: March 2022

D-1,2,4-Butanetriol (BT) has attracted much attention for its various applications in energetic materials and the pharmaceutical industry. Here, a synthetic pathway for the biosynthesis of BT from d-arabinose was constructed and optimized in Escherichia coli. First, E. coli Trans1-T1 was selected for the synthesis of BT. Considering the different performance of the enzymes from different organisms when expressed in E. coli, the synthetic pathway was optimized. After screening two d-arabinose dehydrogenases (ARAs), two d-arabinonate dehydratases (ADs), four 2-keto acid decarboxylases (ADXs), and three aldehyde reductases (ALRs), ADG from Burkholderia sp., AraD from Sulfolobus solfataricus, KivD from Lactococcus lactis IFPL730, and AdhP from E. coli were selected for the bio-production of BT. After 48 h of catalysis, 0.88 g/L BT was produced by the recombinant strain BT5. Once the enzymes were selected for the pathway, metabolic engineering strategy was conducted for further improvement. The final strain BT5ΔyiaEΔycdWΔyagE produced 1.13 g/L BT after catalyzing for 48 h. Finally, the fermentation conditions and characteristics of BT5ΔyiaEΔycdWΔyagE were also evaluated, and then 2.24 g/L BT was obtained after 48 h of catalysis under the optimized conditions. Our work was the first report on the biosynthesis of BT from d-arabinose which provided a potential for the large-scale production of d-glucose-based BT.
Prediction of Femoral Strength Based on Bone Density and Biochemical Markers in Elderly Men With Type 2 Diabetes Mellitus
Authors: Jia Shaowei , Gong He , Zhang Yingying , Liu Hongmei , Cen Haipeng , Zhang Rui , Fan Yubo
Published: March 2022

Purpose: Effects of bone density, bone turnover and advanced glycation end products (AGEs) on femoral strength (FS) are still unclear in patients with type 2 diabetes mellitus (T2DM). This study aims to assess and predict femoral strength and its influencing factors in elderly men with T2DM.

Methods: T2DM patients (n = 10, mean age, 66.98 years) and age-matched controls (n = 8, mean age, 60.38 years) were recruited. Femoral bone mineral density (BMD) and serum biochemical indices of all subjects were measured. FS was evaluated through finite element analysis based on quantitative computed tomography. Multiple linear regression was performed to obtain the best predictive models of FS and to analyze the ability of predictors of FS in both groups.

Results: FS (p = 0.034), HbA1c (p = 0.000) and fasting blood glucose (p = 0.000) levels of T2DM group were significantly higher than those of control group; however, the P1NP level (p = 0.034) was significantly lower. FS was positively correlated with femoral neck T score (FNTS) (r = 0.794, p < 0.01; r = 0.881, p < 0.01) in both groups. FS was correlated with age (r = -0.750, p < 0.05) and pentosidine (r = -0.673, p < 0.05) in T2DM group. According to multiple linear regression, FNTS and P1NP both contributed to FS in two groups. P1NP significantly improved the prediction of FS in both groups, but significant effect of FNTS on predicting FS was only presented in control group. Furthermore, pentosidine, age and HbA1c all played significant roles in predicting FS of T2DM.

Conclusion: Femoral strength was higher in elderly men with T2DM, which might be caused by higher BMD and lower bone turnover rate. Moreover, besides BMD and bone formation level, AGEs, blood glucose and age might significantly impact the prediction of femoral strength in T2DM.
Targeting Cancer Cell Ferroptosis to Reverse Immune Checkpoint Inhibitor Therapy Resistance
Authors: Deng Jingjing , Zhou Mei , Liao Tingting , Kuang Wenlong , Xia Hui , Yin Zhengrong , Tan Qi , Li Yumei , Song Siwei , Zhou E , Jin Yang
Published: March 2022

In recent years, cancer therapies using immune checkpoint inhibitors (ICIs) have achieved meaningful success, with patients with advanced tumors presenting longer survival times and better quality of life. However, several patients still do not exhibit good clinical outcomes for ICI therapy due to low sensitivity. To solve this, researchers have focused on identifying the cellular and molecular mechanisms underlying resistance to ICI therapy. ICI therapy induces apoptosis, which is the most frequent regulated cell death (RCD) but lacks immunogenicity and is regarded as an “immune silent” cell death. Ferroptosis, a unique type of non-apoptotic-RCD, has been preliminarily identified as an immunogenic cell death (ICD), stimulating tumor-antigen-specific immune responses and augmenting anti-tumor immune effects. However, ferroptosis has rarely been used in clinical practice. Present evidence strongly supports that the interferon-γ signaling pathway is at the crossroads of ICI therapy and ferroptosis. TYRO3, a receptor tyrosine kinase, is highly expressed in tumors and can induce anti-programmed cell death (PD)-ligand 1/PD-1 therapy resistance by limiting tumoral ferroptosis. Therefore, in this review, we summarize the clinical practice and effects of ICI therapy in various cancers. We also provide an overview of ferroptosis and report the molecular connections between cancer cell ferroptosis and ICI therapy, and discuss the possibility to reverse ICI therapy resistance by inducing cancer cell ferroptosis.
A Minimal Model Shows that a Positive Feedback Loop Between sNHE and SLO3 can Control Mouse Sperm Capacitation
Authors: de Prelle Bertrand , Lybaert Pascale , Gall David
Published: March 2022

When mammalian spermatozoa are released in the female reproductive tract, they are incapable of fertilizing the oocyte. They need a prolonged exposure to the alkaline medium of the female genital tract before their flagellum gets hyperactivated and the acrosome reaction can take place, allowing the sperm to interact with the oocyte. Ionic fluxes across the sperm membrane are involved in two essential aspects of capacitation: the increase in intracellular pH and the membrane hyperpolarization. In particular, it has been shown that the SLO3 potassium channel and the sNHE sodium-proton exchanger, two sperm-specific transmembrane proteins, are necessary for the capacitation process to occur. As the SLO3 channel is activated by an increase in intracellular pH and sNHE is activated by hyperpolarization, they act together as a positive feedback system. Mathematical modeling provides a unique tool to capture the essence of a molecular mechanism and can be used to derive insight from the existing data. We have therefore developed a theoretical model formalizing the positive feedback loop between SLO3 and sHNE in mouse epididymal sperm to see if this non-linear interaction can provide the core mechanism explaining the existence of uncapacited and capacitated states. We show that the proposed model can fully explain the switch between the uncapacitated and capacited states and also predicts the existence of a bistable behaviour. Furthermore, our model indicates that SLO3 inhibition, above a certain threshold, can be effective to completely abolish capacitation.
The Impact of Metabolic Syndrome and Type 2 Diabetes Mellitus on Prostate Cancer
Authors: Sousa André P. , Costa Raquel , Alves Marco G. , Soares Raquel , Baylina Pilar , Fernandes Rúben
Published: March 2022

Prostate cancer (PCa) remains the second most common type of cancer in men worldwide in 2020. Despite its low death rate, the need for new therapies or prevention strategies is critical. The prostate carcinogenesis process is complex and multifactorial. PCa is caused by a variety of mutations and carcinogenic events that constitutes the disease’s multifactorial focus, capable of not only remodeling cellular activity, but also modeling metabolic pathways to allow adaptation to the nutritional requirements of the tumor, creating a propitious microenvironment. Some risk factors have been linked to the development of PCa, including Metabolic Syndrome (MetS) and Type 2 Diabetes Mellitus (T2DM). MetS is intrinsically related to PCa carcinogenic development, increasing its aggressiveness. On the other hand, T2DM has the opposite impact, although in other carcinomas its effect is similar to the MetS. Although these two metabolic disorders may share some developmental processes, such as obesity, insulin resistance, and dyslipidemia, their influence on PCa prognosis appears to have an inverse effect, which makes this a paradox. Understanding the phenomena behind this paradoxical behavior may lead to new concepts into the comprehension of the diseases, as well as to evaluate new therapeutical targets. Thus, this review aimed to evaluate the impact of metabolic disorders in PCa’s aggressiveness state and metabolism.
The Role of Impaired Mitochondrial Dynamics in MFN2-Mediated Pathology
Authors: Zaman Mashiat , Shutt Timothy E.
Published: March 2022

The Mitofusin 2 protein (MFN2), encoded by the MFN2 gene, was first described for its role in mediating mitochondrial fusion. However, MFN2 is now recognized to play additional roles in mitochondrial autophagy (mitophagy), mitochondrial motility, lipid transfer, and as a tether to other organelles including the endoplasmic reticulum (ER) and lipid droplets. The tethering role of MFN2 is an important mediator of mitochondrial-ER contact sites (MERCs), which themselves have many important functions that regulate mitochondria, including calcium homeostasis and lipid metabolism. Exemplifying the importance of MFN2, pathogenic variants in MFN2 are established to cause the peripheral neuropathy Charcot-Marie-Tooth Disease Subtype 2A (CMT2A). However, the mechanistic basis for disease is not clear. Moreover, additional pathogenic phenotypes such as lipomatosis, distal myopathy, optic atrophy, and hearing loss, can also sometimes be present in patients with CMT2A. Given these variable patient phenotypes, and the many cellular roles played by MFN2, the mechanistic underpinnings of the cellular impairments by which MFN2 dysfunction leads to disease are likely to be complex. Here, we will review what is known about the various functions of MFN2 that are impaired by pathogenic variants causing CMT2A, with a specific emphasis on the ties between MFN2 variants and MERCs.
A Theoretical Study on the Structural, Electronic, and Magnetic Properties of Bimetallic Pt13−nNin (N = 0, 3, 6, 9, 13) Nanoclusters to Unveil the Catalytic Mechanisms for the Water-Gas Shift Reaction
Authors: Barrionuevo Manoel Victor Frutuoso , Andrés Juan , San-Miguel Miguel Angel
Published: March 2022

In this work, first-principles calculations by using density functional theory at the GFN-xTB level, are performed to investigate the relative stability and structural, electronic, and magnetic properties of bimetallic Pt13−nNin (n = 0, 3, 6, 9, 13) nanoclusters by using corrected Hammer and Nørskov model. In addition, by employing the reaction path and the energetic span models, the energy profile and the turnover frequency are calculated to disclose the corresponding reaction mechanism of the water-gas shift reaction catalyzed by these nanoclusters. Our findings render that Ni causes an overall shrinking of the nanocluster’s size and misalignment of the spin channels, increasing the magnetic nature of the nanoclusters. Pt7Ni6 nanocluster is the most stable as a result of the better coupling between the Pt and Ni d-states. Pt4Ni9 maintains its structure over the reaction cycle, with a larger turnover frequency value than Pt7Ni6. On the other hand, despite Pt10Ni3 presenting the highest value of turnover frequency, it suffers a strong structural deformation over the completion of a reaction cycle, indicating that the catalytic activity can be altered.
Preliminary Observation of the Changes in the Intestinal Flora of Patients With Graves’ Disease Before and After Methimazole Treatment
Authors: Yang Mengxue , Zheng Xiaodi , Wu Yueyue , Zhang Rui , Yang Qian , Yu Zhiyan , Liu Jun , Zha Bingbing , Gong Qihai , Yang Bo , Sun Bowen , Zeng Miao
Published: March 2022

Immune dysfunction caused by environmental factors plays an important role in the development of Graves’ disease (GD), and environmental factors are closely related to the intestinal flora. Our previous study showed significant changes in the intestinal flora in GD patients compared with healthy volunteers. This study analyzed the relationships between changes in the intestinal flora, thyroid function and relevant thyroid antibodies in GD patients before and after methimazole treatment. The subjects were divided into the UGD group (18 newly diagnosed GD patients), the TGD group (10 GD patients with normal or approximately normal thyroid function after methimazole treatment) and the NC group (11 healthy volunteers). Their fresh stool samples were sent for 16S rRNA gene amplification and Illumina platform sequencing. The correlations of the relative abundance of Bifidobacterium with the levels of TRAb, TgAb and TPOAb in the NC group and the UGD group were analyzed. A total of 1,562,445 high-quality sequences were obtained. In the UGD group, the abundances of Bifidobacterium and Collinsella were higher than that in the NC group; Bacteroides abundance in the TGD group was higher than that in the NC group, while Prevotella and Dialister abundances were lower than that in the NC group; Prevotella and Collinsella abundances in the UGD group were higher than that in the TGD group. The predominant abundance distribution of Bifidobacteriaceae in the UGD group at the family level was superior to that in the NC group. The abundance of Bifidobacterium was positively correlated with the levels of TRAb, TgAb, and TPOAb. The biological diversity of the intestinal flora was reduced in GD patients. After methimazole treatment, the composition of the intestinal flora was significantly altered. The change in Bifidobacterium abundance was positively correlated with TRAb, TgAb and TPOAb, suggesting that it might be related to the immune mechanism of GD. The results of this study may deepen our understanding of the pathogenesis of GD and provide a new idea for the treatment of GD.
The Use of Comparative Genomic Analysis for the Development of Subspecies-Specific PCR Assays for Mycobacterium abscessus
Authors: Akwani Winifred C. , van Vliet Arnoud H.M. , Joel Jordan O. , Andres Sönke , Diricks Margo , Maurer Florian P. , Chambers Mark A. , Hingley-Wilson Suzanne M.
Published: March 2022

Mycobacterium abscessus complex (MABC) is an important pathogen of immunocompromised patients. Accurate and rapid determination of MABC at the subspecies level is vital for optimal antibiotic therapy. Here we have used comparative genomics to design MABC subspecies-specific PCR assays. Analysis of single nucleotide polymorphisms and core genome multilocus sequence typing showed clustering of genomes into three distinct clusters representing the MABC subspecies M. abscessus, M. bolletii and M. massiliense. Pangenome analysis of 318 MABC genomes from the three subspecies allowed for the identification of 15 MABC subspecies-specific genes. In silico testing of primer sets against 1,663 publicly available MABC genomes and 66 other closely related Mycobacterium genomes showed that all assays had >97% sensitivity and >98% specificity. Subsequent experimental validation of two subspecies-specific genes each showed the PCR assays worked well in individual and multiplex format with no false-positivity with 5 other mycobacteria of clinical importance. In conclusion, we have developed a rapid, accurate, multiplex PCR-assay for discriminating MABC subspecies that could improve their detection, diagnosis and inform correct treatment choice.
The Microbiology of Acute Exacerbations in Chronic Rhinosinusitis - A Systematic Review
Authors: Okifo Oghenefejiro , Ray Amrita , Gudis David A.
Published: March 2022

Background

Acute exacerbations (AE) in chronic rhinosinusitis (CRS) are a common and important clinical issue. However, relatively little is known regarding the underlying microbiology that drives exacerbations or how it relates to the microbiome of CRS. The purpose of this study is to examine the literature to characterize the microbiome associated with acute exacerbations in a chronic rhinosinusitis setting. Understanding this disease process may facilitate targeted antibiotic therapy, reduced antibiotic resistance, and offer more effective disease control and treatment efficacy.

Objective

To characterize the microbiome associated with acute exacerbations of chronic rhinosinusitis (AECRS).

Methods

We conducted a systematic review of the literature on Medline, Embase, and Web of Science databases from January 1990-June 2021 to identify studies related to AE in CRS. Exclusion criteria include non-English, non-human studies, and case reports. Studies without culture or PCR data were also excluded.

Results

Fourteen studies were identified which provided detailed data regarding sinus microbiome in AECRS patients. In these patients, a total of 1252 individual isolates were identified. While common acute pathogens were identified in high frequencies in the sinonasal cultures (Staphylococcus pneumonia, Haemophilus influenza), the predominant bacteria were Staphylococcus aureus (including methicillin-sensitive Staphylococcus aureus) and Pseudomonas aeruginosa. Patient characteristics that may represent higher risk phenotypes were not consistently collected in the studies. Discussion of antimicrobial sensitivities and/or resistance were included in 7/14 studies.

Conclusions

This systematic review identifies the predominant microbiology species that may contribute to AECRS. Further studies are needed to understand the pathogenic role of bacteria and viruses in AECRS and to identify associated comorbidities and patient phenotypes that may predispose to AE. The optimal treatment regimen for AECRS remains unclear.
The COVID-19 Pandemic – A Diagnostic Industry Perspective
Authors: Das Shubhagata , Dunbar Sherry
Published: March 2022

The COVID-19 pandemic has brought about unprecedented changes to all facets of the healthcare system, including the diagnostic industry. The pandemic has highlighted both the challenges and strengths of the industry and has also provided valuable insights on how to be better prepared for future pandemics. In this perspective article, we describe the challenges faced by the diagnostic industry in general, particularly the difficulties encountered by Luminex Corporation, a diagnostic assay development and manufacturing company located in Austin, Texas, USA, as well as the mitigation strategies employed. In addition to discussion of the key challenges, the article provides insights on the lessons learned and steps that can be undertaken to better prepare for future outbreaks.
Injury and Healing Response of Healthy Peripheral Arterial Tissue to Intravascular Lithotripsy: A Prospective Animal Study
Authors: Liu Feng , Ge Yangyang , Rong Dan , Zhu Yating , Yin Jianhan , Sun Guoyi , Jia Xin , Guo Wei
Published: March 2022

Objectives

Intravascular lithotripsy (IVL) is a novel clinical technique for the management of severely calcified lesions. However, the biological effects of shock wave on the healthy arterial tissue have not been demonstrated. The preclinical safety study aimed to investigate the vascular response to IVL shock wave compared to plain old balloon angioplasty (POBA) in porcine peripheral arteries.

Methods

The left and right iliofemoral arterial segments of 16 mini-pigs were subjected to IVL and POBA, respectively. The vascular response was evaluated using quantitative vascular angiography (QVA), light microscopy, and scanning electron microscopy (SEM) at 0, 5, and 28 days.

Results

With the emission of shock wave, adjacent muscle contraction was observed. QVA showed there was no statistically significant difference in percent diameter stenosis and late lumen loss between the two groups. SEM examination showed the endothelial cell layer was intact in both groups at all timepoints. Under light microscopy, no area stenosis was observed. However, IVL shock wave resulted in significantly higher percent area stenosis and intimal area at 28 days. Neointima score showed a trend toward a higher rate in the IVL group, although there was no statistically significant difference at 28 days. There were no statistically significant differences in the scored parameters between groups at all timepoints. However, the parameters of inflammation and neointima showed a trend toward higher scores in the IVL group. After disruption of the internal elastic lamina, the arteries demonstrated significantly neointimal thickening.

Conclusions

The safety and operability of IVL are comparable to POBA. The histological response of healthy arteries to IVL shock wave is mild and sustained. IVL shock wave do not cause serious vascular tissue damage, especially endothelial denudation.
Midterm Outcome After Septal Myectomy and Medical Therapy in Mildly Symptomatic Patients With Hypertrophic Obstructive Cardiomyopathy
Authors: Sun Jiejun , Liang Lin , Li Peijin , Jiang Tengyong , Yu Xianpeng , Ren Changwei , Dong Ran , He Jiqiang
Published: March 2022

Objective

The purpose of this study was mainly to determine the midterm outcome of septal myectomy (SM) and medical therapy (MT) in mildly symptomatic patients (NYHA class II) with hypertrophic obstructive cardiomyopathy (HOCM).

Methods

The study cohort consisted of 184 mildly symptomatic patients with HOCM evaluated in Beijing Anzhen Hospital, Capital Medical University between March 2001 and December 2017, including 82 patients in the SM group and 102 patients in the MT group. Overall survival and HCM-related survival were mainly observed.

Results

The average follow-up time was 5.0 years. Compared to patients accepting MT, patients treated with SM were associated with comparable overall survival (96.5% and 93.1% vs. 92.9% and 83.0% at 5 and 10 years, respectively; P = 0.197) and HCM-related survival (98.7% and 98.7% vs. 94.2% and 86.1% at 5 and 10 years, respectively; P = 0.063). However, compared to MT, SM was superior at improvement of NYHA class (1.3 ± 0.6 vs. 2.1 ± 0.5, P < 0.001) and mean reduction of resting left ventricular outflow (LVOT) gradient (78.5 ± 18.6% vs. 28.3 ± 18.4%, P < 0.001). Multivariate analysis suggested that resting LVOT gradient in the last clinical examination was an independent predictor of all-cause mortality (HR = 1.017, 95%CI: 1.000–1.034, P = 0.045) and HCM-related mortality (HR = 1.024, 95%CI: 1.005–1.043, P = 0.012) in the entire cohort.

Conclusion

Compared with MT, SM had comparable overall survival and HCM-related survival in mildly symptomatic HOCM patients, but SM had advantages on improving clinical symptoms and reducing resting LVOT gradient. Resting LVOT gradient in the last clinical examination was an independent predictor of all-cause mortality and HCM-related mortality.
Nd and Sr Isotopes and REE Investigation in Tropical Weathering Profiles of Amazon Region
Authors: Horbe Adriana Maria Coimbra , Albuquerque Márcio Fernando Dos Santos , Dantas Elton Luiz
Published: March 2022

The exceptional development of lateritic profiles make the Amazon one of the best places to conduct a refined study on the effects of long-term tropical climate on the Earth's surface rocks. Concentrations of Nd and Sr isotopes, as well as rare earth elements (REE), Sr, and other trace elements were determined in six profiles in order to shed new light into element behavior and into the processes controlling the geochemistry and the transfer of these elements among the different compartments of the critical zone. Our investigation indicates natural isotope regional pulse transformations in the Oxisol at the top of all profiles. The ƐNd(t=0) values of the parent rock, mottled horizon, and lateritic duricrust are radiogenic similar to the Amazon craton signature. The thick Oxisol has ƐNd(t=0) values which are much more radiogenic and less variable, whereas it has Zr, Th, Y, and REE in higher concentrations. These issues highlight a complex process for the Nd system, and the following possibilities are proposed to explain this behavior: formation of authigenic cerianite and/or kaolinite, penetration of low-temperature weathering solutions along zircon fractures, and some vegetation uptake. These possibilities allow keeping the more radiogenic Nd isotopes, whereas the less radiogenic Nd is released especially in the Oxisol after REE mineral(s) weathering. The Sr system contrary to that of Nd became less radiogenic along the profiles and relatively to the parent rocks content, because nearly all Sr was removed after K-Rb feldspar and mica weathering. However, a mixture return of less radiogenic Sr from plant, biogenic aerosol, and rainwater may have helped let the weathering profiles become less radiogenic. These characteristics let the Oxisol even less Sr radiogenic but still permitting to identify the general parent rocks signature. In contrast, Nd is highly fractionated in the Oxisol relative to the parent rock. Thus, the REE and Sr behavior, similar in the six profiles, does not necessarily reflect only the parent rock geochemistry, but it also depends on the multiple surficial processes typical of the critical zone: weathering, pedogenesis, plant root activity, and rainwater composition which change the inherited primary minerals isotope signatures.
Landslides, a Key Landform in the Global Geological Heritage
Authors: Morino Costanza , Coratza Paola , Soldati Mauro
Published: March 2022

Landslides are important components of global geoheritage, but awareness of their significance and value in such terms seems scanty in the scientific community. Landslides are normally identified among various features of geological and geomorphological interest, and often considered a source of hazard. However, they are seldom identified as geosites and as part of geoheritage. This paper aims at filling these gaps by highlighting the importance of landslides in the global geoheritage. After a short introduction on the values and criteria to define landforms as geosites, based on literature review, we show to what extent landslides have been defined as geomorphosites and as part of geoheritage around the world. We then outline three aspects that should be specifically considered in the identification of landslides as geomorphosites, namely 1) past and present climate changes, 2) anthropic signature, and 3) risk perception. Finally, we describe four cases of spectacular landslides that serve as significant examples worldwide.
Fracture Characteristics and its Role in Bedrock Reservoirs in the Kunbei Fault Terrace Belt of Qaidam Basin, China
Authors: Wang Zhaosheng , Xiang Hui , Wang Libin , Xie Lin , Zhang Zhenguo , Gao Lianfeng , Yan Zhifeng , Li Fuling
Published: March 2022

Fracture characteristics at bedrock reservoirs in the Kunbei fault terrace belt in the southwest of Qaidam Basin, China, are investigated based on cores, thin sections, formation microscanner imaging, and production data. Results show that the weathered unit at the top of bedrocks is not an effective reservoir because of extremely low porosity. A semi-weathered unit is a potential reservoir, where formation water migrating along early effective fractures could form dissolution fractures and pores as primary storage space, and fractures can also act as seepage channels. Four fracture types can be identified from bedrock reservoirs, e.g., structural ones, diagenetic ones, weathering ones, and dissolution ones, while shear fractures related to faults are the most important ones. Fracture types and characteristics are different in granite and slate, e.g., high fracture density results in intensive dissolution in granite. Fracture density is closely related to tectonic stress. The bedrock reservoirs near fault zones and semi-weathered units are potential oil and gas exploration targets in the Kunbei fault terrace belt. Effective fractures govern dissolution behaviors as well as dominant seepage directions, which play a significant role in the development of bedrock reservoirs.
Belief in Counseling Service Effectiveness and Academic Self-Concept as Correlates of Academic Help-Seeking Behavior Among College Students
Authors: Bizuneh Shambel
Published: March 2022

This study aimed at understanding students’ academic self-concepts, academic help-seeking behaviors, and beliefs in counseling service effectiveness. Based on a correlational research design, a closed-ended questionnaire was administrated to 182 college students. An independent-sample t-test revealed that the average scores of male students were significantly higher than average scores of female students in academic self-concept, help-seeking behavior, and beliefs in counseling effectiveness. An analysis of the relationship between them confirmed that academic help-seeking behaviors, beliefs in counseling service effectiveness, and academic self-concepts significantly correlated with each other. This study also revealed that the variance of academic self-concept and belief in counseling service effectiveness contributed to 36% of the variance in academic help-seeking behavior. Therefore, enhancement projects on academic self-concept and female students’ belief in the effectiveness of counseling services should be taken as an agenda by teachers, college administrators, academic advisors, and counselors.
Impact of Insulin Resistance on Cardiometabolic Risk Factors and an Anthropometry-Based Predictive Nomogram for Insulin Resistance Among Adolescents in China
Authors: Du Runyu , Li Ling , Li Ping , Wang Yanjun
Published: March 2022

Objective

We aimed to investigate the impact of insulin resistance (IR), as determined by the homeostasis model assessment of insulin resistance (HOMA-IR), on cardiometabolic risk factors (CMRFs), and develop an anthropometry-based predictive nomogram for IR among adolescents in China.

Design

Data were acquired from a cross-sectional study with a stratified cluster sampling method, conducted among adolescents in Northeast China.

Participants

A total of 882 adolescents (aged 12–16 years, 468 boys) were included.

Measurements

All participants underwent anthropometric and biochemical examinations. The thresholds of IR included the 90th percentile of the HOMA-IR for adolescents with a normal body mass index (BMI) and fasting plasma glucose (FPG) level within each sex group (Cutoff A), and the 75th percentile for all participants of the same sex (Cutoff B).

Results

The HOMA-IR was associated with CMRFs. IR, as defined by both cutoffs A and B, was significantly associated with most CMRFs, except decreased HDL-C levels. Excellent concordance (κ = 0.825) was found between these two criteria in diagnosing IR. However, IR using cutoff A, was more closely associated with cardiometabolic risk. The incidence of IR, as defined by cutoff A, was 18.93% and increased from 10.99% to 43.87% based on the different BMI categories. Further, an anthropometry-based predictive model for IR, incorporating sex, age, waist-to-hip ratio, weight and BMI, was developed and presented as a nomogram.

Conclusions

IR among adolescents is strongly related to cardiometabolic risk. We developed an anthropometry-based predictive nomogram for IR among adolescents, which may facilitate health counselling and self-risk assessments.
Electro-Osmotic Flow of Water–Methanol Mixtures Through Nafion Membranes Revisited: Composition and Temperature Dependence
Authors: Corti Horacio R. , Trevani Liliana
Published: March 2022

The electro-osmotic drag coefficient of water and methanol mixtures through Nafion 117 membranes was measured as a function of the composition at several temperatures between 25 and 60°C using a two-compartment capillary cell with Ag/AgCl electrodes. The electro-osmotic water drag in HCl aqueous solutions is higher than that reported in measurements where the membrane is in contact with pure water; hence, all the reported results were performed at the same acid concentration. It was found that the drag coefficient for pure methanol is about 40% higher than that for water at all the temperatures studied as a consequence of the expanded nanostructure of Nafion in methanol. The drag coefficients of the water–methanol mixtures exhibit a high non-linearity, which can be explained by considering the Nafion sorption in the binary solvent. The electro-osmotic flow in pure methanol is similar to that of 5 M methanol aqueous solutions, which opens the opportunity to use pure methanol in DMFCs. The methanol crossover due to permeability can be minimized. Controversial results with previous studies are also addressed.
Numerical and Experimental Investigations of Modal Coherence Characteristics for the Determination of Broadband Noise in Flow Ducts
Authors: Xu Kunbo , Liu Cunliang , Tong Fan , Qiao Weiyang
Published: March 2022

This paper presents a detailed investigation about the modal coherence characteristics of turbomachinery noise. The modal coherence characteristics of ducted noise at high frequencies are investigated by establishing three kinds of ducted sound source distribution models. The results are consistent with the acoustic experimental results of a single-stage axial fan. The results show that acoustic modes of different orders are mutually incoherent, and radial modes within the same circumferential mode have a great degree of coherence. This paper successfully conducted an acoustic measurement on turbomachinery noise by rotating microphone arrays. The acoustic experimental results show that the coherence of some modes has little impact on the measurement of incident acoustic waves in the flow duct, while it leads to about 3 dB deviation in the prediction of the reflected modal sound power and has little influence on the spectral shape of reflected acoustic waves. It was demonstrated that the experimentally measured modal coherence functions give an inner sense of the spatial noise source distribution and enable the investigation of mode scattering within the propagation. Based on the modal coherence characteristics obtained in this paper, more theoretical and methodological studies can be carried out for the turbomachinery broadband noise test.
Effect of Dimethyl Formamide (DMF) on Vanadium Reloading Over V-Ti SCR Catalyst
Authors: Song Hao , Guo Jiangmin , Liu Shaojun , Zhang Yu , Wu Weihong , Zheng Chenghang , Gao Xiang
Published: March 2022

Active components reloading is critical process for the catalyst regeneration, which is limited by the low adsorption capacity and unwilling distribution of desired components to the catalyst surface. Herein we demonstrated that with dimethyl formamide(DMF) modification and sequentially reloading of vanadium, the traditional V-Ti SCR catalyst, which uses vanadium as the active components and titanium as the carrier, showed the significantly improved DeNOx performance, owing to the increased adsorption capacity and desired distribution of vanadium. When the DMF concentration was 6%, the adsorption capacity of the promoted catalyst was 3.58 and 6.57 mg/g under vanadyl ion concentrations of 1.5 and 3 g/L, respectively, 135 and 147% higher than that of the original catalyst. Adsorption kinetics demonstrated that the pseudo-second-order kinetic model better describes the process by which vanadyl ions adsorb onto the catalyst. In addition, the adsorption equilibrium indicated that Langmuir model was a closer fit for the vanadyl ion adsorption to the promoted catalyst. After DMF modification, the vanadyl ions were first adsorbed onto the functional groups on the catalyst surface, substantially increasing the vanadium loading on the catalyst surface while limiting the increase in vanadium content within the interior of catalyst, which was conducive to enhancing the DeNOx activity and reducing the increase in the SO2/SO3 conversion. When the vanadium adsorption capacity was 3.5 mg/g, the increase in the DeNOx activity of the promoted catalyst was 68.1% higher than that of the original catalyst, whereas the increase in SO2/SO3 conversion was 28.9% lower than that of the original catalyst. Thus, in the regeneration of SCR catalysts vanadium initial concentration and loading could be reduced.
Methodological Comparison of the Production Approach 2013 and 2019 for Quantifying the Carbon Stock in Harvested Wood Products in China
Authors: Yu Zhihan , Zhang Han , Tu Qingshi , Yang Hongqiang
Published: March 2022

Harvested wood products (HWP) play an important role in global climate change mitigation. The Production Approach, an internationally accepted HWP carbon accounting approach by the Intergovernmental Panel on Climate Change (IPCC), has been revised several times to enhance the accuracy of the accounting results. This study compared the methodological differences in the Production Approach elaborated in the 2013 Revised Supplementary Methods and Good Practice Guidance Arising from the Kyoto Protocol (PA2013) and 2019 Refinement to the 2006 IPCC Guidelines for National Greenhouse Gas Inventories (PA2019). We used PA2019 to calculate the HWP carbon stock in China under different wood utilization scenarios. In terms of recycling and reusing of recovered paper, we found that PA2019 has a more accurate representation of the increasing proportion of paper and paperboard materials produced using recovered paper in the world. In 2019, the HWP carbon stock in China was 815.94 Mt (1 Mt = 106 t) using PA2019. In detail, the carbon stock of sawnwood, wood-based panels, and paper and paperboard were 208.52 Mt, 559.73 Mt, and 47.69 Mt, respectively. Scenario simulation results show that half-life was an important factor that affecting HWP carbon stock. China can improve HWP carbon stock by increasing the recovered paper utilization rate for paper products or increasing the utilization rate of HWP with long half-life in construction (structural material). The additional carbon stock obtained by changing the utilization structure of wood and improve the half-life of HWP will not be large until decades later but nonetheless important.
The Impact of Economic Growth and Tiered Medical Policy on the Medical Waste Generation: An Empirical Analysis Based on the Environmental Kuznets Curve Model
Authors: Ma Yan , Jia Linlin , Hou Yanhong , Wu Xiaoming
Published: March 2022

Medical waste has a high environmental risk, economic development and medical policies can affect medical waste generation. Per capita gross domestic product (GDP) is an important indicator to measure the macroeconomic development status, and the tiered medical policy plays an important role in the process of China’s medical reform. The study considered the Environmental Kuznets Curve (EKC) of medical waste generation (MWG) in eight cities in China from 2013 to 2019, using economic indicator (GDP per capita) and policy indicator (tiered medical policy) to reveal the impact of economic development and medical policy on MWG. Furthermore, residents, especially the elderly, are the main consumers of medical resources, and medical institutions are the main places where medical waste is generated. Education level can affect waste management practices. Therefore, the research introduced population size, number of medical institutions, aging degree and education level as control variables. The results confirmed the validity of the N-shaped EKC curve between medical waste and GDP per capita. The amount of medical waste would continue to increase with economic growth, but the growth rate was not fixed. Implementing the tiered medical policy had shown a trend towards reducing medical waste. Population size and aging degree positively affected the generation of medical waste. The number of medical institutions negatively affected the generation of medical waste. The effect of education level on MWG could be ignored. Based on the empirical analysis results, this research put forward relevant policy suggestions in the light of medical waste management practice in China.
Metabolite Profiling of the Social Spider Stegodyphus dumicola Along a Climate Gradient
Authors: Sandfeld Tobias , Malmos Kirsten Gade , Nielsen Camilla Bak , Lund Marie Braad , Aagaard Anne , Bechsgaard Jesper , Wurster Martina , Lalk Michael , Johannsen Mogens , Vosegaard Thomas , Bilde Trine , Schramm Andreas
Published: March 2022

Animals experience climatic variation in their natural habitats, which may lead to variation in phenotypic responses among populations through local adaptation or phenotypic plasticity. In ectotherm arthropods, the expression of thermoprotective metabolites such as free amino acids, sugars, and polyols, in response to temperature stress, may facilitate temperature tolerance by regulating cellular homeostasis. If populations experience differences in temperatures, individuals may exhibit population-specific metabolite profiles through differential accumulation of metabolites that facilitate thermal tolerance. Such thermoprotective metabolites may originate from the animals themselves or from their associated microbiome, and hence microbial symbionts may contribute to shape the thermal niche of their host. The social spider Stegodyphus dumicola has extremely low genetic diversity, yet it occupies a relatively broad temperature range occurring across multiple climate zones in Southern Africa. We investigated whether the metabolome, including thermoprotective metabolites, differs between populations, and whether population genetic structure or the spider microbiome may explain potential differences. To address these questions, we assessed metabolite profiles, phylogenetic relationships, and microbiomes in three natural populations along a temperature gradient. The spider microbiomes in three genetically distinct populations of S. dumicola showed no significant population-specific pattern, and none of its dominating genera (Borrelia, Diplorickettsia, and Mycoplasma) are known to facilitate thermal tolerance in hosts. These results do not support a role of the microbiome in shaping the thermal niche of S. dumicola. Metabolite profiles of the three spider populations were significantly different. The variation was driven by multiple metabolites that can be linked to temperature stress (e.g., lactate, succinate, or xanthine) and thermal tolerance (e.g., polyols, trehalose, or glycerol): these metabolites had higher relative abundance in spiders from the hottest geographic region. These distinct metabolite profiles are consistent with a potential role of the metabolome in temperature response.