bluuebunny/crossref_metadata_2025 huggingface dataset:
GSK872 and necrostatin-1 protect retinal ganglion cells against necroptosis through inhibition of RIP1/RIP3/MLKL pathway in glutamate-induced retinal excitotoxic model of glaucoma
Authors: Liu Mengyuan , Li Haibo , Yang Rongliang , Ji Dan , Xia Xiaobo
Published: October 2022

Background

Glaucoma, the major cause of irreversible blindness worldwide, is characterized by progressive degeneration of retinal ganglion cells (RGCs). Current treatments for glaucoma only slow or partially prevent the disease progression, failing to prevent RGCs death and visual field defects completely. Glutamate excitotoxicity via N-methyl-d-aspartic acid (NMDA) receptors plays a vital role in RGCs death in glaucoma, which is often accompanied by oxidative stress and NLRP3 inflammasome activation. However, the exact mechanisms remain unclear.

Methods

The glutamate-induced R28 cell excitotoxicity model and NMDA-induced mouse glaucoma model were established in this study. Cell counting kit-8, Hoechst 33342/PI dual staining and lactate dehydrogenase release assay were performed to evaluate cell viability. Annexin V-FITC/PI double staining was used to detect apoptosis and necrosis rate. Reactive oxygen species (ROS) and glutathione (GSH) were used to detect oxidative stress in R28 cells. Levels of proinflammatory cytokines were measured by qRT-PCR. Transmission electron microscopy (TEM) was used to detect necroptotic morphological changes in RGCs. Retinal RGCs numbers were detected by immunofluorescence. Hematoxylin and eosin staining was used to detect retinal morphological changes. The expression levels of RIP1, RIP3, MLKL and NLRP3 inflammasome-related proteins were measured by immunofluorescence and western blotting.

Results

We found that glutamate excitotoxicity induced necroptosis in RGCs through activation of the RIP1/RIP3/MLKL pathway in vivo and in vitro. Administration of the RIP3 inhibitor GSK872 and RIP1 inhibitor necrostatin-1 (Nec-1) prevented glutamate-induced RGCs loss, retinal damage, neuroinflammation, overproduction of ROS and a decrease in GSH. Furthermore, after suppression of the RIP1/RIP3/MLKL pathway by GSK872 and Nec-1, glutamate-induced upregulation of key proteins involved in NLRP3 inflammasome activation, including NLRP3, pro-caspase-1, cleaved-caspase-1, and interleukin-1β (IL-1β), was markedly inhibited.

Conclusions

Our findings suggest that the RIP1/RIP3/MLKL pathway mediates necroptosis of RGCs and regulates NLRP3 inflammasome activation induced by glutamate excitotoxicity. Moreover, GSK872 and Nec-1 can protect RGCs from necroptosis and suppress NLRP3 inflammasome activation through inhibition of RIP1/RIP3/MLKL pathway, conferring a novel neuroprotective treatment for glaucoma.
Inflammation and cell-to-cell communication, two related aspects in frailty
Authors: Pansarasa Orietta , Mimmi Maria Chiara , Davin Annalisa , Giannini Marta , Guaita Antonio , Cereda Cristina
Published: October 2022

Background

Frailty is a complex, multi-dimensional age-related syndrome that increases the susceptibility to adverse health outcomes and poor quality of life. A growing consensus supports the contribution of chronic inflammation and immune system alterations to frailty, however a clear role of such alterations remains to be elucidated. Furthermore, pro- and anti-inflammatory cytokines together with other signaling molecules might spread from activated cells to the adjacent ones through extracellular vesicles (EVs), which have also a role in cellular aging. The aim of the present research was to investigate if EVs play a role in the immune function in frailty. 

Results

In 219 older adults aged 76–78 years, selected from the InveCe.Ab study (Abbiategrasso, Italy), we investigated inflammation and EVs-mediated intercellular communication. C-reactive protein (CRP) and pro- (IL-1β, IL-2, IL-6, IL-8, IL-12 p70, TNFα and IFNγ) and anti- (IL-4, IL-10, IL-13) inflammatory cytokines were evaluated on plasma of Frail and non-Frail subjects. We reported a significant increase in CRP, interleukin-1β and -6 (IL-1β, IL-6) and tumor necrosis factor alpha (TNFα) plasma levels in frailty. In female Fr subjects, we also reported an increase in interferon‐gamma (IFN‐γ) and, surprisingly, in IL-13, an anti-inflammatory cytokine, whose increase seems to oppose the inflammaging theory.

An inflammatory panel (toll-like receptors 2 and 4 (TLR2 and TLR4), tumor necrosis factor receptors TNFRec5/CD 40 and TNFRec1B/CD120B) and a panel including receptors involved in cellular senescence (insulin-like growth factor 1 receptor (CD221) and interleukin 6 receptor (IL-6R)) were indeed analysed in plasma isolated large EVs (lEVs) from Frail (n = 20) and non-Frail (n = 20) subjects. In lEVs isolated from plasma of Frail subjects we reported an increase in TLR2 and TLR4, TNFRec5/CD 40 and TNFRec1B/CD120B, suggesting a chronic state of inflammation. In addition, CD221 and IL-6R increases in lEVs of Frail individuals.

Conclusions

To conclude, the pro-inflammatory status, notably the increase in circulating cytokines is pivotal to understand the potential mechanisms underlying the frailty syndrome. Moreover, cytokines release from EVs, mainly the large ones, into the extracellular space suggest their contribution to the formation of a pro-inflammatory and pro-senescent microenvironment that, in turn, can contribute to frailty.
Spinal microglia contribute to sustained inflammatory pain via amplifying neuronal activity
Authors: Gu Nan , Yi Min-Hee , Murugan Madhuvika , Xie Manling , Parusel Sebastian , Peng Jiyun , Eyo Ukpong B. , Hunt Christine L. , Dong Hailong , Wu Long-Jun
Published: October 2022

Microglia are highly dynamic immune cells of the central nervous system (CNS). Microglial processes interact with neuronal elements constantly on the order of minutes. The functional significance of this acute microglia-neuron interaction and its potential role in the context of pain is still largely unknown. Here, we found that spinal microglia increased their process motility and electrophysiological reactivity within an hour after the insult in a mouse model of formalin-induced acute, sustained, inflammatory pain. Using an ablation strategy to specifically deplete resident microglia in the CNS, we demonstrate that microglia participate in formalin-induced acute sustained pain behaviors by amplifying neuronal activity in the spinal dorsal horn. Moreover, we identified that the P2Y12 receptor, which is specifically expressed in microglia in the CNS, was required for microglial function in formalin-induced pain. Taken together, our study provides a novel insight into the contribution of microglia and the P2Y12 receptor in inflammatory pain that could be used for potential therapeutic strategies.
Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose
Authors: Wang Ling-Ru , Zhang Zi-Xu , Nong Fang-Tong , Li Jin , Huang Peng-Wei , Ma Wang , Zhao Quan-Yu , Sun Xiao-Man
Published: October 2022

Background

Schizochytrium sp. is a heterotrophic, oil-producing microorganism that can efficiently produce lipids. However, the industrial production of bulk chemicals using Schizochytrium sp. is still not economically viable due to high-cost culture medium. Replacing glucose with cheap and renewable lignocellulose is a highly promising approach to reduce production costs, but Schizochytrium sp. cannot efficiently metabolize xylose, a major pentose in lignocellulosic biomass.

Results

In order to improve the utilization of lignocellulose by Schizochytrium sp., we cloned and functionally characterized the genes encoding enzymes involved in the xylose metabolism. The results showed that the endogenous xylose reductase and xylulose kinase genes possess corresponding functional activities. Additionally, attempts were made to construct a strain of Schizochytrium sp. that can effectively use xylose by using genetic engineering techniques to introduce exogenous xylitol dehydrogenase/xylose isomerase; however, the introduction of heterologous xylitol dehydrogenase did not produce a xylose-utilizing engineered strain, whereas the introduction of xylose isomerase did. The results showed that the engineered strain 308-XI with an exogenous xylose isomerase could consume 8.2 g/L xylose over 60 h of cultivation. Xylose consumption was further elevated to 11.1 g/L when heterologous xylose isomerase and xylulose kinase were overexpressed simultaneously. Furthermore, cultivation of 308-XI-XK(S) using lignocellulosic hydrolysates, which contained glucose and xylose, yielded a 22.4 g/L of dry cell weight and 5.3 g/L of total lipid titer, respectively, representing 42.7 and 30.4% increases compared to the wild type.

Conclusion

This study shows that engineering of Schizochytrium sp. to efficiently utilize xylose is conducive to improve its utilization of lignocellulose, which can reduce the costs of industrial lipid production.
Phase Ib Study of Telisotuzumab Vedotin in Combination With Erlotinib in Patients With c-Met Protein–Expressing Non–Small-Cell Lung Cancer
Authors: Camidge D. Ross , Barlesi Fabrice , Goldman Jonathan W. , Morgensztern Daniel , Heist Rebecca , Vokes Everett , Spira Alex , Angevin Eric , Su Wu-Chou , Hong David S. , Strickler John H. , Motwani Monica , Dunbar Martin , Parikh Apurvasena , Noon Elysa , Blot Vincent , Wu Jun , Kelly Karen
Published: October 2022

PURPOSE

Overexpression of c-Met protein and epidermal growth factor receptor ( EGFR) mutations can co-occur in non–small-cell lung cancer (NSCLC), providing strong rationale for dual targeting. Telisotuzumab vedotin (Teliso-V), a first-in-class antibody-drug conjugate targeting c-Met, has shown a tolerable safety profile and antitumor activity as monotherapy. Herein, we report the results of a phase Ib study (ClinicalTrials.gov identifier: NCT02099058 ) evaluating Teliso-V plus erlotinib, an EGFR tyrosine kinase inhibitor (TKI), in patients with c-Met–positive (+) NSCLC.

PATIENTS AND METHODS

This study evaluated Teliso-V (2.7 mg/kg once every 21 days) plus erlotinib (150 mg once daily) in adult patients (age ≥ 18 years) with c-Met+ NSCLC. Later enrollment required presence of an EGFR-activating mutation ( EGFR-M +) and progression on a prior EGFR TKI. End points included safety, pharmacokinetics, objective response rate (ORR), and progression-free survival (PFS). The efficacy-evaluable population consisted of c-Met+ patients (confirmed histology [H]-score ≥ 150) who had at least one postbaseline scan; c-Met+ patients with H-scores ≥ 225 were classified as c-Met high.

RESULTS

As of January 2020, 42 patients were enrolled (N = 36 efficacy-evaluable). Neuropathies were the most common any-grade adverse events reported, with 24 of 42 patients (57%) experiencing at least one event. The pharmacokinetic profile of Teliso-V plus erlotinib was similar to Teliso-V monotherapy. Median PFS for all efficacy-evaluable patients was 5.9 months (95% CI, 2.8 to not reached). ORR for EGFR-M + patients (n = 28) was 32.1%. Of EGFR-M + patients, those who were c-Met high (n = 15) had an ORR of 52.6%. Median PFS was 6.8 months for non-T790M+ and for those whose T790M status was unknown, versus 3.7 months for T790M+.

CONCLUSION

Teliso-V plus erlotinib showed encouraging antitumor activity and acceptable toxicity in EGFR TKI-pretreated patients with EGFR-M+, c-Met+ NSCLC.
The Finnish genetic heritage in 2022 – from diagnosis to translational research
Authors: Uusimaa Johanna , Kettunen Johannes , Varilo Teppo , Järvelä Irma , Kallijärvi Jukka , Kääriäinen Helena , Laine Minna , Lapatto Risto , Myllynen Päivi , Niinikoski Harri , Rahikkala Elisa , Suomalainen Anu , Tikkanen Ritva , Tyynismaa Henna , Vieira Päivi , Zarybnicky Tomas , Sipilä Petra , Kuure Satu , Hinttala Reetta
Published: October 2022

Isolated populations have been valuable for the discovery of rare monogenic diseases and their causative genetic variants. Finnish disease heritage (FDH) is an example of a group of hereditary monogenic disorders caused by single major, usually autosomal-recessive, variants enriched in the population due to several past genetic drift events. Interestingly, distinct subpopulations have remained in Finland and have maintained their unique genetic repertoire. Thus, FDH diseases have persisted, facilitating vigorous research on the underlying molecular mechanisms and development of treatment options. This Review summarizes the current status of FDH, including the most recently discovered FDH disorders, and introduces a set of other recently identified diseases that share common features with the traditional FDH diseases. The Review also discusses a new era for population-based studies, which combine various forms of big data to identify novel genotype–phenotype associations behind more complex conditions, as exemplified here by the FinnGen project. In addition to the pathogenic variants with an unequivocal causative role in the disease phenotype, several risk alleles that correlate with certain phenotypic features have been identified among the Finns, further emphasizing the broad value of studying genetically isolated populations.
Is the Interpolated-Twitch Technique-Derived Voluntary Activation Just Neural? Novel Perspectives from Mechanomyographic Data
Authors: CORATELLA GIUSEPPE , CÈ EMILIANO , DORIA CHRISTIAN , BORRELLI MARTA , TONINELLI NICHOLAS , RAMPICHINI SUSANNA , LIMONTA ELOISA , LONGO STEFANO , ESPOSITO FABIO
Published: October 2022

Purpose

Voluntary activation (VA) determined by interpolation-twitch technique could be affected by the characteristics of the in-series elastic components. To overcome this possible bias, a novel approach based on the mechanomyographic (MMG) signal to detect voluntary activation (VAMMG) has been proposed. We examined the changes in VA and VAMMG after passive stretching to check the influence of neural and mechanical factors in the force output.

Methods

Twenty-six healthy men underwent VA assessment using the interpolated-twitch technique before and after unilateral passive stretching of the plantarflexors (five 45-s on + 15-s off). In addition to the force signal, the MMG signal was detected on gastrocnemius medialis, gastrocnemius lateralis, and soleus. From the force and MMG signal analysis, VA and VAMMG were calculated in the stretched and contralateral nonstretched limbs. Joint passive stiffness was also defined.

Results

In the stretched limb, passive stretching increased dorsiflexion range (mean ± SD = +18% ± 10%, P < 0.001, ES = 1.54) but reduced joint passive stiffness (−22% ± 8%, P < 0.001, ES = −1.75), maximum voluntary contraction (−15% ± 7%, P < 0.001, ES = −0.87), VA (−7% ± 3%, P < 0.001, ES = −2.32), and VAMMG (~−5% ± 2%, P < 0.001, ES = −1.26/−1.14). In the contralateral nonstretched limb, passive stretching increased dorsiflexion range (+10% ± 6%, P < 0.001, ES = 0.80) but reduced joint passive stiffness (−3% ± 2%, P = 0.041, ES = −0.27), maximum voluntary contraction (−4% ± 3%, P = 0.035, ES = −0.24), VA (−4% ± 2%, P < 0.001, ES = −1.77), and VAMMG (~− 2% ± 1%, P < 0.05, ES = −0.54/−0.46). The stretch-induced changes in VA correlated with VAMMG (R ranging from 0.447 to 0.583 considering all muscles) and with joint passive stiffness (stretched limb: R = 0.503; contralateral nonstretched limb: R = 0.530).

Conclusions

VA output is overall influenced by both neural and mechanical factors, not distinguishable using the interpolated-twitch technique. VAMMG is a complementary index to assess the changes in VA not influenced by mechanical factors and to examine synergistic muscles.
Persistent Polarizing Effects of Persuasion: Experimental Evidence from Turkey
Authors: Baysan Ceren
Published: October 2022

I evaluate randomly varied neighborhood exposure to information campaigns regarding either executive performance, or increases in executive power, prior to a Turkish referendum on weakening checks and balances on the executive. The campaigns increased voter polarization over the referendum, and subsequently changed party affiliation in national and local elections over the next two years, leading to partisan polarization. My results suggest that, when voters disagree on whether increasing executive power is a good policy, more information can increase voter polarization. Finally, I conclude that because potential polarization is often ignored, the impact of information campaigns on civil society is underestimated. (JEL D72, D83, O17)
Evaluating Deliberative Competence: A Simple Method with an Application to Financial Choice
Authors: Ambuehl Sandro , Bernheim B. Douglas , Lusardi Annamaria
Published: October 2022

We examine methods for evaluating interventions designed to improve decision-making quality when people misunderstand the consequences of their choices. In an experiment involving financial education, conventional outcome metrics (financial literacy and directional behavioral responses) imply that two interventions are equally beneficial even though only one reduces the average severity of errors. We trace these failures to violations of the assumptions embedded in the conventional metrics. We propose a simple, intuitive, and broadly applicable outcome metric that properly differentiates between the interventions, and is robustly interpretable as a measure of welfare loss from misunderstanding consequences even when additional biases distort choices. (JEL D83, D91, G51, G53)
Social Media and Mental Health
Authors: Braghieri Luca , Levy Ro’ee , Makarin Alexey
Published: October 2022

We provide quasi-experimental estimates of the impact of social media on mental health by leveraging a unique natural experiment: the staggered introduction of Facebook across US colleges. Our analysis couples data on student mental health around the years of Facebook’s expansion with a generalized difference-in-differences empirical strategy. We find that the rollout of Facebook at a college had a negative impact on student mental health. It also increased the likelihood with which students reported experiencing impairments to academic performance due to poor mental health. Additional evidence on mechanisms suggests the results are due to Facebook fostering unfavorable social comparisons. (JEL D91, I12, I23, L82)
Immigration and Spatial Equilibrium: The Role of Expenditures in the Country of Origin
Authors: Albert Christoph , Monras Joan
Published: October 2022

We document that international migrants concentrate more in expensive cities—the more so, the lower the prices in their origin countries are—and consume less locally than comparable natives. We rationalize this empirical evidence by introducing a quantitative spatial equilibrium model, in which a part of immigrants’ income goes toward consumption in their origin countries. Using counter-factual simulations, we show that, due to this novel consumption channel, immigrants move economic activity toward expensive, high-productivity locations. This leads to a more efficient spatial allocation of labor and, as a result, increases the aggregate output and welfare of natives. (JEL F24, J15, J31, J61, R23)
Phantasma-agoria of/in crisis
Authors: Πασχαλίδου Μαρία
Published: October 2022

Στην διδακτορική μου διατριβή, που εντάσσεται στο πλαίσιο της καλλιτεχνικής έρευνας, διερευνώ τρόπους με τους οποίους ένα καλλιτεχνικό γεγονός μπορεί να μετασχηματίσει το δημόσιο χώρο, την αγορά, από έναν τόπο εμπορευματοποίησης και κατανάλωσης σε ένα χώρο που ενεργοποιεί το πολιτικό, διαταράσσοντας την τάξη αυτού που κάθε φορά επιτρέπεται να είναι ορατό και μπορεί να ειπωθεί. Η έρευνα πραγματοποιήθηκε κατά την περίοδο 2012-2016 και πληροφορήθηκε άμεσα από το ιστορικό-κοινωνικό πλαίσιο αυτής της περιόδου. Ειδικότερα, θέτει ως πεδίο προβληματισμού τις πολιτικές της αισθητικής που κυριάρχησαν την περίοδο της οικονομικής και ανθρωπιστικής κρίσης στην Αθήνα και τις καλλιτεχνικές πρακτικές που βρίσκονταν στο προσκήνιο ως πιο ‘αντιπροσωπευτικές’ για την απόδοση του πολιτικού. Τα καλλιτεχνικά γεγονότα τις περισσότερες φορές βασίζονταν σε συμμετοχικά δρώμενα, χωρίς ιδιαίτερους οπτικούς πειραματισμούς, ενώ τα οπτικά μέσα περιορίζονταν στην τεκμηριωτική καταγραφή των συνεπειών της κρίσης.Για να διερευνήσω την σχέση μεταξύ οπτικότητας και συμμετοχικών πρακτικών, χρησιμοποίησα κριτικά την έννοια της φαντασμαγορίας, η οποία παραδοσιακά συλλαμβάνεται ως το σύμβολο του καταναλωτικού πολιτισμού. Συγκεκριμένα, επικεντρώθηκα στη σημασιολογία της λέξης ως σύνθεσης του φαντάσματος [εικόνα] και της αγοράς [δημόσιος χώρος/λόγος], για να επανεξετάσω την πρακτική της συμμετοχικότητας συσχετίζοντάς την με την πολιτική και την οπτικότητα. Συνδυάζοντας οπτικά μέσα και ψηφιακές τεχνολογίες με τη συμμετοχικότητα και την επιτέλεση, τα καλλιτεχνικά έργα, που δημιουργήθηκαν στο πλαίσιο αυτής της έρευνας, παράγουν συσχετίσεις εικόνας/φαντάσματος και αγοράς, δημιουργώντας όρους για πολιτική σκέψη και πράξη. Λαμβάνοντας υπόψη ότι η λέξη κρίση στην ελληνική γλώσσα σημαίνει, μεταξύ άλλων, κριτική σκέψη, αυτά τα έργα συνιστούν τη Φαντασμαγορία σε κρίση, δηλαδή μια κριτική προσέγγιση της φαντασμαγορίας. Ταυτόχρονα, αποτελούν και μια Φαντασμαγορία της κρίσης, καθώς αναφέρονται στην οικονομική κρίση στην Ελλάδα και στους τρόπους με τους οποίους πλαισίωσε νέες α/ορατότητες στην αγορά. Στην πραγματικότητα, κάθε ένα από τα έργα αυτά διερευνά οπτικά διαφορετικές πτυχές της έννοιας της αγοράς: τη διάρθρωση της αγοράς μέσω του λόγου και της γλώσσας, τη διάρθρωση της αγοράς μέσω συλλογικής δράσης, τη χωρική πολιτική της αγοράς σε σχέση με το δίπολο ιδιωτικού (ή οικιακού) και δημόσιου χώρου. Με την εννοιολόγηση της πρακτικής της συμμετοχικότητας μέσω της ιδέας της αγοράς, η καλλιτέχνις και οι συμμετέχουσες/συμμετέχοντες, εμπλέκονται σε έναν διάλογο πολιτικής εγρήγορσης. Ανάλογα με την περίπτωση, η καλλιτέχνιδα θα πρέπει να είναι πρόθυμη να αναλάβει το ρίσκο και να περάσει μέρος της εξουσίας της στις/στους συμμετέχουσες/συμμετέχοντες και το αντίστροφο. Η ανάληψη του ρίσκου εγκαθιδρύει εμπιστοσύνη και ενισχύει την αντίσταση σε κανονιστικές πρακτικές της εμπορευματοποίησης του πολιτισμού, θέτοντας σε κίνηση την πολιτική αγορά.
Πρόγραμμα ελέγχου και παρέμβασης για την ορθή χρήση των αντιμηκυτιακών φαρμάκων στο Γ.Ν.Α. "ΛΑΪΚΟ"
Authors: Κοραντάνης Κωνσταντίνος
Published: October 2022

Στόχοι: Να εκτιμηθεί ο αντίκτυπος ενός προγράμματος αντιμυκητιακής διαχείρισης (ΠΑΔ) στην κατάλληλη χρήση, το κόστος κατανάλωσης και απόκτησης αντιμυκητιακών και τα κλινικά αποτελέσματα (νοσοκομειακή θνησιμότητα, διάρκεια παραμονής στο νοσοκομείο). Μέθοδοι: Η μελέτη διεξήχθη σε πανεπιστημιακό νοσοκομείο τριτοβάθμιας περίθαλψης 535 κλινών και είχε τρεις διαδοχικές περιόδους. Α) Περίοδος παρατήρησης (10 μήνες): όλες οι αντιμυκητιακές συνταγές αξιολογήθηκαν προοπτικά. Β) Εκπαιδευτική παρέμβαση για την αύξηση της ευαισθητοποίησης σχετικά με τη σωστή χρήση των αντιμυκητιακών. Γ) Υλοποίηση ενός μη υποχρεωτικού ΠΑΔ (10 μήνες) βάσει προοπτικού ελέγχου και ανατροφοδότησης πληροφόρησης. Χρησιμοποιήθηκε ανάλυση διακοπής χρονοσειρών για την αξιολόγηση του αντίκτυπου της παρέμβασης. Αποτελέσματα: Κατά την προ-επεμβατική περίοδο, 198 συνταγογραφήσεις αντιμυκητιακών για 147 ασθενείς, αξιολογήθηκαν σε σύγκριση με 181 συνταγές σε 138 ασθενείς κατά τη διάρκεια της περιόδου ΠΑΔ . Η στατιστική ανάλυση έδειξε σημαντική άμεση πτώση των ακατάλληλων συνταγών αμέσως μετά την παρέμβαση, με παραμονή της πτωτικής τάσης στη συνέχεια, καθώς και μια παρατεταμένη σημαντική πτώση στη συνολική κατανάλωση αντιμυκητιακών αμέσως μετά την παρέμβαση και σημαντική πτωτική τάση μετέπειτα. Όλη η νοσοκομειακή θνητότητα ήταν σταθερή κατά την περίοδο πριν από την παρέμβαση με σημαντική πτωτική τάση μετά την εφαρμογή του προγράμματος ΠΑΔ. Η σύγκριση των προ- και μετά την παρέμβαση περιόδων έδειξε σημαντική μείωση του κόστους (-26,8%, p <0,001) αλλά καμία διαφορά ως προς τον συνολικό αριθμό των ημερών (107.654 έναντι 102.382) και τη μέση διάρκεια της παραμονής στο νοσοκομείο ( 5,19 έναντι 4,96 ημέρες, p = NS). Συμπεράσματα: Η εφαρμογή ενός μη υποχρεωτικού προγράμματος ΠΑΔ είχε ως αποτέλεσμα τη σημαντική βελτίωση της ποιότητας των συνταγών και τη μείωση της κατανάλωσης αντιμυκητιακών και του κόστους αγοράς, χωρίς να επηρεάζεται η συνολική θνητότητα στο νοσοκομείο και η μέση διάρκεια παραμονής στο νοσοκομείο.
Time-lapse monitoring of pollution by geoelectrical methods
Authors: Κίρκου Στέλλα
Published: October 2022

Αντικείμενο της διατριβής είναι η μελέτη της αποτελεσματικότητας της μεθοδολογίας της διαχρονικής γεωηλεκτρικής τομογραφίας για την παρακολούθηση στη μελέτη διάχυσης ρύπων στο υπέδαφος. Στόχος είναι η ανάπτυξη μεθοδολογιών ώστε να συνδεθεί ποιοτικά αλλά και ποσοτικά η διαχρονική γεωηλεκτρική εικόνα με την αποτύπωση ιδιαίτερων χαρακτηριστικών της υπεδάφιας ρύπανσης. Η μελέτη επικεντρώθηκε στη μελέτη ρύπων που προέρχονται από τα απόβλητα των ελαιοτριβείων λόγω της ιδιαίτερης σημασίας που έχει αυτού του τύπου η ρύπανση στον Ελληνικό χώρο. Με δεδομένη την ιδιαίτερη σημασία που έχουν στη διαδικασία παρακολούθησης τόσο ο τρόπος λήψης διαχρονικών μετρήσεων όσο και η επεξεργασία τους η παρούσα μελέτη επικεντρώθηκε στην εύρεση βέλτιστων συνδυασμών μετρήσεων και επεξεργασίας ώστε η παρακολούθηση της ρύπανσης να είναι πιο αποτελεσματική.Η διατριβή προτείνει μιας λειτουργική πειραματική διάταξη και μια διαδικασία επεξεργασίας διαχρονικών μετρήσεων ηλεκτρικής τομογραφίας με ηλεκτρόδια σε γεωτρήσεις αλλά και στην επιφάνεια για την παρακολούθηση της διάχυσης των ρύπων στο υπέδαφος. Η μεθοδολογία δοκιμάστηκε με επιτυχία σε διαφορετικές κλίμακες. Ειδικότερα μελετήθηκαν διάφορες παράμετροι της γεωμετρίας λήψης των μετρήσεων και της επεξεργασίας αυτών μέσω συνθετικών δεδομένων προσομοίωσης. Επίσης έγινε προσομοίωση διάχυσης ρύπου στο υπέδαφος με υδρογεωλογικά μοντέλα και έγινε μετατροπή των προσομοιωμάτων της ρύπανσης σε τρισδιάστατες γεωηλεκτρικές εικόνες για τη λήψη συνθετικών διαχρονικών γεωηλεκτρικών μετρήσεων και τον έλεγχο της δυνατότητας της μεθόδου παρακολούθησης να μετρήσει την ταχύτητα διάχυσης του ρύπου. Ακολούθησε η λήψη και ειδική επεξεργασία διαχρονικών γεωηλεκτρικών δεδομένων σε απόλυτα ελεγχόμενο περιβάλλον (δεξαμενή προσομοίωσης 1 m³) με διάχυση ρύπου σε μικρή κλίμακα για την οποία υπολογίστηκε η ταχύτητα διάχυσης του ρύπου.Τέλος έγινε μεταφορά όλης της μεθοδολογίας σε μεγαλύτερη κλίμακα για την εκτέλεση παρόμοιων με το βασικό πείραμα μετρήσεων σε έναν ειδικά διαμορφωμένο εξωτερικό χώρο δοκιμών όπου και πάλι υπολογίστηκε η ταχύτητα διάχυσης του ρύπου.
3D mechanical metamaterial scaffolds for tissue engineering applications
Authors: Φλαμουράκης Γεώργιος
Published: October 2022

Η πολυφωτονική λιθογραφία είναι μια ισχυρή τεχνική που επιτρέπει την κατασκευή δομών σε mικροκλίμακα με πρωτοφανή ανάλυση. Εκμεταλλευόμενοι τις εξαιρετικές μηχανικές ιδιότητες της ρητίνης SZ2080, καταφέραμε να κατασκευάσουμε και να χαρακτηρίσουμε δύο κύρια μηχανικά μεταϋλικά: μία αυξητική δομή, (επίσης γνωστή ως bowtie) που παρουσιάζει αυξητικές ιδιότητες και το εξαιρετικά άκαμπτο υπερελαφρύ τετραδεκάεδρο (επίσης γνωστός ως αφρός Kelvin) που εμφανίζει πολύ υψηλή σκληρότητα και είναι αποτελούμενος κυρίως από κενό χώρο. Με την αλλαγή της αρχιτεκτονικής κυψελίδων μονάδας, δημιουργήθηκαν αυτά τα δύο διαφορετικά μηχανικά περιβάλλοντα, παρόλο που το αρχικό υλικό ήταν το ίδιο.Αρχικά, το υλικό SZ2080 χαρακτηρίστηκε μηχανικά με μικρο-εσοχή και η bowtie δομή βελτιστοποιήθηκε, κατασκευάστηκε και χρησιμοποιήθηκε ως ικρίωμα για κυτταρικές μελέτες της κυτταρικής σειράς ινοβλαστών NIH-3T3. Τα κύτταρα μπόρεσαν να διαπεράσουν τους πόρους των ικριωμάτων, τα παραμόρφωσαν και αύξησαν τον πολλαπλασιασμό τους.Δεύτερον, αντιμετωπίστηκε ο υψηλός αυτοφθορισμός του πολυμερούς. Για το λόγο αυτό, συντέθηκε μια νέα παραλλαγή του SZ2080 που χαρακτηρίζεται από την αλλαγή του φωτοεκκινητή σε Sudan Black B (SBB). Με αυτόν τον τρόπο, κατασκευάστηκαν μεγάλα ικριώματα μη φθορισμού και χρησιμοποιήθηκαν με Μεσεγχυματικά Βλαστοκύτταρα (MSC). Στη συνέχεια χρησιμοποιήθηκε ομοεστιακή μικροσκοπία για τη λήψη στοίβων z και τη χαρτογράφηση ολόκληρου του όγκου του ικριώματος που σπάρθηκε με MSC.Το τρίτο μέρος αφορούσε τη μελέτη της οστεογονικής διαφοροποίησης των MSCs σε αυξητικό περιβάλλον. Δύο κύριες πρωτεΐνες παρακολουθήθηκαν με ομοεστιακή μικροσκοπία και PCR: η πρωτεΐνη μηχανο-επαγωγής YAP-1 και ο οστεογενής δείκτης Runx2. Επιπλέον, η μορφολογία των κυττάρων αναλύθηκε μέσω Ηλεκτρονικής Μικροσκοπίας Σάρωσης (SEM). Τα αποτελέσματα έδειξαν ότι η διαδικασία οστεογονικής διαφοροποίησης ενισχύεται στο αυξητικό περιβάλλον σε σύγκριση με την επίπεδη 2D καλλιέργεια.Τέλος, ο τρισδιάστατος εκτυπωτής στερεολιθογραφίας 1 φωτονίου χρησιμοποιήθηκε για την παραγωγή ικριωμάτων τριπλής περιοδικής ελάχιστης επιφάνειας μεγάλης κλίμακας (δομή Swartz G ή γυροειδής) με κελιά μονάδας ανάλυσης 200μm και έγινε καλλιέργεια με 3 διαφορετικές κυτταρικές σειρές. Είναι ενδιαφέρον ότι δεν παρατηρήθηκε κυτταροτοξικότητα και τα κύτταρα προσαρτήθηκαν και πολλαπλασιάστηκαν σε όλα τα ικριώματα.Συμπερασματικά, τα αυξητικά ικριώματα είναι ιδανικά για οστεογονική διαφοροποίηση καθώς μπορούν να διατηρήσουν και να προωθήσουν αποτελεσματικά την οστεογένεση ακόμη και μετά από 28 ημέρες καλλιέργειας. Αυτή η εργασία ανοίγει το δρόμο για τη χρήση πιο περίπλοκων μεταϋλικών στο πεδίο της μηχανικής ιστών και ωθεί τα όρια για το τι μπορεί να χρησιμοποιηθεί η κατασκευή με τη βοήθεια φωτός.
The state of individual components of humoral immunity of guinea pig blood in the dynamics of the formation of experimental periodontitis and immobilization stress and correction of their disorders by thiocetam
Authors: Regeda M. , Olekshij P.
Published: October 2022

The research found a rapid increase in the level of immunoglobulin M, starting from the 3 rd day of the experiment in comparison with the control values, which clearly indicates its activation in particular, and stimulation of humoral immunity in general. In the later stages (on the 5 th and 15 th  days) of the formation of experimental periodontitis (EP) and immobilization stress (IS), an increase of this indicator by 93.5% and 94.2% was found, respectively, against group I guinea pigs (p≤0, 05).Regarding the content of immunoglobulin G in the blood, the highest growth rates were recorded in the fourth group of guinea pigs with EP and IS (on the 15 th day), which indicates a direct dependence of the duration of damaging factors on the level of these indicators and the body's ability to the protective response. The results of the treatment showed the effect and the reduction of the activity of the studied classes of immunoglobulins. Thus, the use of thiocetam led to a significant decrease in the content of Ig M and G in the blood, respectively, by 33.8% (p1≤0.05) and 41.7% (p1≤0.05) in EP and IS compared with the group of animals were not exposed to this drug, which indicates the immunocorrective effect of this drug on the studied tests.
Od metafizycznej neutralności do metafizyki problemów. Status metafizyki w filozofii Husserla
Authors: Łaciak Piotr
Published: October 2022

Od metafizycznej neutralności do metafizyki problemów.

Status metafizyki w filozofii Husserla

 

Streszczenie

 

Artykuł prezentuje przejście filozofii Husserla od psychologii fenomenologicznej do fenomenologii transcendentalnej jako zwrot metafizyczny. Husserlowska fenomenologia przedtranscendentalna jako deskryptywna analiza fenomenów mentalnych wyklucza wszelkie metafizyczne pytania dotyczące istnienia świata zewnętrznego, podczas gdy fenomenologia transcendentalna nie jest metafizycznie neutralna, ponieważ ma za zadanie odsłonięcie ostatecznego sensu świata w jego faktycznym istnieniu. Z jednej strony, zgodnie z pierwszeństwem ejdetycznych możliwości przed rzeczywistością, fenomenologia transcendentalna jako nauka ejdetyczna poprzedza metafizykę jako naukę o rzeczywistości i umożliwia ją. Z tego punktu widzenia fenomenologia transcendentalna może być rozumiana w znaczeniu ostatecznego ugruntowania metafizyki jako uniwersalnej i absolutnej nauki o faktach, która jest zwieńczeniem naturalnych nauk o bycie. Z drugiej strony, zasada pierwszeństwa istoty przed faktem nie znajduje zastosowania do transcendentalnego faktu konstytucji świata i ten irracjonalny fakt prowadzi do metafizyki jako filozofii dotyczącej „najwyższych i ostatecznych zagadnień”, takich jak teleologiczne, teologiczne i etyczne zagadnienia. Zagadnienia te okazują się irracjonalną resztą (metafizycznym residuum) fenomenologii transcendentalnej i jej zwieńczeniem.

 

Słowa kluczowe: ejdetyczne możliwości, fakt absolutny, fenomenologia transcendentalna, metafizyka krytyczna, metafizyka problemów, nauka o faktach
Dwa pokolenia szkoły lwowsko-warszawskiej: Tadeusz Czeżowski (1889-1981) i Zbigniew Jordan (1911-1977)
Authors: Konstańczak Stefan
Published: October 2022

Artykuł w całości nawiązuje do dotąd niepublikowanej korespondencji Tadeusza Czeżowskiego z emigracyjnym filozofem Zbigniewem Jordanem zachowanej w rękopisach w zasobach archiwalnych Uniwersytetu Mikołaja Kopernika w Toruniu. Ich korespondencja trwała blisko 40 lat i jest dowodem, że nawet przymusowa powojenna emigracja nie zrywała więzów łączących filozofów związanych ze szkołą lwowsko-warszawską. Choć Czeżowski i Jordan należeli do dwóch różnych pokoleń tej szkoły, to wspólne zainteresowania naukowe i podobne zapatrywania polityczne spowodowały, że ich więź, choć tylko korespondencyjna,  była w pewnym sensie wyjątkowa, bo nawet ingerencje komunistycznej cenzury nie były w stanie wpłynąć na jej kształt. Dzięki niej Jordan wykazywał się doskonałą orientacją w dorobku samego Czeżowskiego jak i pozostałych przedstawicieli szkoły lwowsko-warszawskiej. To właśnie głównie dzięki tej korespondencji mógł pełnić rolę „ambasadora” polskiej filozofii w świecie i zarazem pierwszego historyka szkoły lwowsko-warszawskiej. Przypomnieniu roli obu filozofów w dziejach polskiej filozofii sprzyja zwłaszcza przypadająca w tym roku 40-ta rocznica śmierci Czeżowskiego oraz 110 rocznica urodzin Jordana.
Wierność prawdzie warunkiem bycia podmiotem moralnym.Porównanie koncepcji Tadeusza Stycznia i Alaina Badiou
Authors: Rejewska Julia
Published: October 2022

Współczesna etyka coraz rzadziej odwołuje się do kategorii prawdy – z tego powodu zainteresowanie budzić może każda propozycja etyczna, której twórca umieszcza kategorię prawdy w samym jej centrum. Na gruncie polskim taką etyką prawdy jest teoria Tadeusza Stycznia (1931-2010), w której prawda ma charakter normatywny. Do normatywnej mocy prawdy w etyce, choć rozumie on prawdę w zupełnie inny sposób, odwołuje się także francuski filozof Alain Badiou (ur. 1937). Zarówno w myśli Stycznia, jak i Badiou zagadnienie prawdy jest ściśle związane z pojęciem powinności. Nie da się ukryć, że filozofie tych myślicieli są od siebie różne, ale można dostrzec między nimi pewne podobieństwa. Celem tego artykułu jest ukazanie zbieżnych punktów w filozofiach Tadeusza Stycznia i Alaina Badiou.  Obaj myśliciele zdają się twierdzić, że człowiek, który dochowuje wierności odkrytej prawdzie czy wydarzeniu, jako podmiot moralny pozostaje wolny, unika zła oraz jest osobą lub Nieśmiertelny.
FINITE ELEMENT-BASED EVALUATION OF RESIDUAL STRESSES IN CARBON FIBER-REINFORCED POLYMER (CFRP) AT MICROSCALE USING FIBER PUSH-OUT EXPERIMENT
Authors: VU QUY TUNG LINH , SEON GUILLAUME , GHAFFARI SARVENAZ , MAKEEV ANDREW
Published: October 2022

Residual Stresses that develop during manufacturing process of Carbon Fiberreinforced Polymer (CFRP) composites can have negative effects on material strength and fatigue properties. The ability to reliably represent residual stresses in the material microstructure may be essential for accurate prediction of composite material properties using numerical micromodels. In this work, a methodology that uses Finite Element Model Updating (FEMU) and experimental data from Fiber Push-Out experiments is presented for evaluation of microscale residual stress in CFRPs. Recently, Fiber Push-Out experiments with in-situ scanning electron microscope (SEM) imaging conducted by the authors on CFRP specimens revealed significant through-thickness matrix deformation/sink-in in the matrix-rich region after nearby fibers are pushed out, a phenomenon attributed to the release of residual stress after the fiber-interface is broken. To characterize and reproduce residual stress in the Push-Out test samples, a two-step approach is proposed. First, a microscale Finite Element Method (FEM) model is built based on the geometry of the Push-Out area. Second, an FEMU procedure determines effective matrix properties associated with stress build-up that will reproduce the matrix Sink-in value as measured experimentally. In this preliminary work, residual stresses are attributed to the development of thermal strains in the matrix during cool down, and an effective Coefficient of Thermal Expansion (CTE) property for the epoxy material is obtained from the FEMU methodology. FEMU results suggest that epoxy matrix’s common CTE (on the order of 50ppm/oC) is too small to explain the observed matrix Sink-in. Preliminary evaluations suggest high level of residual stress in matrix which might initiate and aggravate microstructure defects, and potentially interfere with fiber-matrix interface properties measurements using the Fiber Push-Out Experiment.
ANALYSIS OF PROGRESSIVE DAMAGE IN 3D WOVEN COMPOSITES USING OVERHEIGHT COMPACT TENSION AND COMPACT COMPRESSION
Authors: SESAR NATHAN , MOLLENHAUER DAVID , RAPKING DAN , PANKOW MARK
Published: October 2022

The objective of the ongoing research is to understand the progressive damage and propagation of cracks in a 3D woven composite. This is part of a larger scale effort to understand the progressive damage response of notched tensile and compressive composites. The current effort has gone extensively into studying the response with unidirectional (UD) and traditional textile composites. This project specifically dives into a 3D orthogonal woven composite and is meant to bolster the ongoing effort with information on how binder fibers can change the dynamic crack path as it progresses from the notch throughout the specimen. A 3D orthogonal preform is infused with resin and tested using compact compression (CC) and over-height compact tension (OCT). 7 Samples of each are tested using a custom load fixture and loaded to varying values to capture damage at different stages of failure. Each sample is studied with digital image correlation (DIC) and X-ray Computed Tomography (CT). It is found that even with existing thermal cracks due to increased temperature cure, that the samples retain toughness and strength and show extensive plasticity.
ADDITIVE MANUFACTURING AND MECHANICS OF MULTISCALE ARCHITECTED FLEXIBLE SYNTACTIC FOAMS
Authors: TEWANI H. R. , HINAUS MEGAN , PRABHAKAR PAVANA
Published: October 2022

Polymer syntactic foam is a lightweight composite consisting of hollow particles, like Glass Micro-Balloons (GMBs) or cenospheres, reinforced in a continuous polymer matrix. Due to their inherent weight-saving characteristics and enhanced mechanical properties, these foams are attractive for use in aerospace and marine industries. Recent advances in additive manufacturing (AM) techniques have enabled the development of complex-shaped parts of syntactic foams and circumvents the need for advanced highcost equipment to produce these parts. Selective Laser Sintering (SLS) is a widely adopted powder-based AM technique used to manufacture 3D parts by sintering polymer powder, and unlike other 3D printing methods, SLS does not require support structures. SLS has been reported to generate a segregated matrix system when used with Thermoplastic Urethane (TPU) in a standalone manner. However, the introduction of GMBs to this manufacturing method has thus far not been extensively studied. Consequently, the influence of GMB parameters on the mechanical response of syntactic foam with a segregated matrix is not fully understood. In this work, we use SLS to fabricate and further investigate the mechanical performance of segregated TPU matrix syntactic foam with different grades and volume fractions of (GMBs). We show for the first time that GMB size drives internal microscale architecture within syntactic foams that manifest as counterintuitive macroscale mechanical responses. That is, GMBs with a diameter larger than gaps between the cell walls of the segregated matrix get lodged between the cell walls while those smaller tend to get lodged inside the cell walls of the segregated matrix. Because of this, larger particles increase the stiffness of the syntactic foams while smaller ones do not contribute to this significantly. On the other hand, larger particles with their lower crushing strength reduce the densification stress of the foam, whereas the foam with smaller particles with higher crushing strength behaved similar to pure TPU but with significantly reduced weight. Overall, we show that coupling hollow particle parameters with print parameters can enable the fabrication of 3D printed syntactic foams with hierarchical tailored architectures and functional properties. These findings can be adapted to the development and design of cores for lightweight sandwich structures in the marine and aerospace industries.
EFFECTIVE THERMAL CONDUCTIVITY FOR FUNCTIONALLY GRADED MATERIALS
Authors: NOMURA SEIICHI , KRISHNA NILABH
Published: October 2022

In this paper, an analytical approach is presented to derive the effective thermal conductivity of a composite reinforced by FGM (Functionally Graded Materials) inclusions of spherical shape. The composite consists of spherical inclusions made of FGM embedded in an infinitely-extended matrix phase subject to uniform heat flux at infinity. The thermal conductivity of the FGM inclusion is assumed to vary linearly inside the FGM. The varying thermal conductivity inside the FGM inclusions results in a temperature distribution in the composite. The effective thermal conductivity is derived based on the exact temperature solution for a single FGM inclusion and by employing the self-consistent approximation. The derived values are found to be within the upper and lower bounds of the rule-ofmixtures.
INVESTIGATION OF INTERFACIAL PROPERTIES OF FLATTENED CARBON NANOTUBES AND AMORPHOUS CARBON
Authors: GAIKWAD PRASHIK S. , KOWALIK MARGARET , DUIN ADRI C. T. VAN , ODEGARD GREGORY M.
Published: October 2022

In this work, Molecular Dynamics (MD) simulations are used to study the interfacial properties (interaction energy, frictional sliding resistance and transverse tension) of flattened carbon nanotubes (flCNTs) – amorphous carbon (AC) as a function of density of AC and flCNT-AC crosslinks. The ReaxFF reactive force field was used to develop the models and predict the interfacial properties.
DESIGNING AN IMPROVED INTERFACE IN GRAPHENE/POLYMER COMPOSITES THROUGH MACHINE LEARNING
Authors: DESHPANDE PRATHAMESH P. , DEMILLE KAREN J. , RAHMAN AOWABIN , GHOSH SUSANTA , SPEAR ASHLEY D. , ODEGARD GREGORY M.
Published: October 2022

The matrix-reinforcement interface has been studied extensively to enhance the performance of polymer matrix composites (PMCs). One commonly practiced approach is functionalization of the reinforcement, which significantly improves the interfacial interaction. A molecular dynamics (MD) and machine learning (ML) workflow is proposed to identify the optimal functionalization parameters that result in improved mechanical performance of a 3-layer graphene nanoplatelet (GNP)/ bismaleimide (BMI) nanocomposite. MD is used to generate the training set for a graph convolutional neural network (GCN). This article reports the MD methodology and an example mechanical response from a pull-out simulation. Upcoming work in the proposed MD-ML workflow for designing a nanocomposite with improved mechanical performance is also discussed.
ESTIMATION OF 3D WOVEN DESIGN SENSITIVITIES USING A RAPID MULTISCALE ANALYSIS TECHNIQUE
Authors: RICKS TRENTON M. , MURTHY PAPPU L. N. , MITAL SUBODH K. , BEDNARCYK BRETT A. , PINEDA EVAN J. , SEGAL KENNETH N.
Published: October 2022

Highly-refined finite element models of three-dimension (3D) woven composite systems currently require excessive computational demands that limit their use in sensitivity analysis, uncertainty quantification, and optimization. An alternative analysis methodology was developed using the NASA Multiscale Analysis Tool (NASMAT) where multiscale models of a 3D woven composite (including inter-tow matrix voids and constituent failure) can be completed on a single central processing unit (CPU) on the order of ~30 s. To develop inputs and validation data for the NASMAT model, coupon and acid-digesting testing and x-ray computed tomography were performed. The NASMAT inputs were parameterized using a set of 25 input variables and distributions. These inputs were randomly sampled to generate a total of 100,000 NASMAT analyses that could be used to understand the influence of different material and geometric properties on the warp and weft-direction stiffness and strength. These analyses (including pre/post-processing) were performed in less than eight hours on a 120 CPU cluster. The computational efficiency of the NASMAT model enabled a sensitivity analysis to be performed, and dominant input variables were able to be identified. Key results were consistent with theoretical and experimental observations for the specific 3D woven system studied in this work.
HEATED ADDITIVELY MANUFACTURED MOLDS FOR THERMOPLASTIC COMPOSITE AUTOMATED FIBER PLACEMENT
Authors: STARK WALTER , HANNON NADIA , YAP TIMOTHY , HEATHMAN NATHANIEL , TEHRANI MEHRAN
Published: October 2022

Thermoplastic composite (TPC) tapes can be consolidated in situ via automated fiber placement (AFP) at relatively high temperatures (>250°C) and local pressures (up to a few MPa). In situ consolidation of TPC parts requires special tooling and heated molds to prevent warping. Creating complex three-dimensional (3D) molds at a low cost and in a short time frame can facilitate TPC AFP adoption and entry into new markets requiring customized parts. The industry standard for mold manufacturing is milling or bending metal stock (typically invar, steel, or aluminum). This process can be costly and have lead times upwards of two months. Additive manufacturing can shorten the lead times significantly. Additively manufactured molds, to be used by TPC AFP, should withstand high temperatures and roller pressures. Current mold generation practices do not have a method of heating the mold surface necessary for dimensional accuracy of TPC tape-based parts. This paper investigates a process to create 3D molds via fused filament fabrication (FFF), a form of material extrusion additive manufacturing (AM), and high-performance materials that can withstand the temperatures and pressures of thermoplastic AFP application. FFF offers customized parts quickly and at a low cost. A laser-assisted AFP robot performed a layup over two heated FFF molds made of short carbon fiber reinforced polyetherketoneketone (CFPEKK), a flat plate and one with a one-axis curvature. These specimens performed well with no noticeable permanent deformation of the mold caused by either the roller or the laser demonstrating the viability of this mold generation process to create 3D molds usable with TPC AFP.