bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of Agri-Food Composts in Almond Organic Production: Effects on Soil and Fruit Quality
Authors: Pérez-Murcia María Dolores , Bustamante María Ángeles , Orden Luciano , Rubio Rosa , Agulló Enrique , Carbonell-Barrachina Ángel A. , Moral Raúl
Published: March 2021

The objective of this work was to study the agronomic effects of the application of agri-food composts in almond trees (Prunus dulcis L.) cultivated in two different areas with different almond varieties (cv. Guara and cv. Ferraduel), with both in organic farming conditions throughout two cropping cycles. To reach this objective, almond production and quality issues (seed production, kernel yield, nutrient contents and kernel quality), as well as several physico-chemical and biological properties of the amended soils at the beginning and at the end of each campaign were studied. The four composts used (C1, C2, C3 and C4) were elaborated mainly using exhausted grape marc (EGM) and other wastes (waste from orange juice production, OW; waste from tomato soup production, TW; cattle manure, CM; and sheep manure, SM). Two treatments were used as control treatments, one based on sheep manure and another without any amendment. The composts used produced similar results in kernel yield and production compared to the traditional treatment (sheep manure). However, the thickness of the kernel was higher in the treatments using composts with exhausted grape marc and sheep manure or tomato soup waste. In relation to the almond quality, few differences related to the effect of treatments were observed, except for the content of proteins, sucrose, some fatty acids and vegetal sterols.
Differential Effects of Low Light Intensity on Broccoli Microgreens Growth and Phytochemicals
Authors: Gao Meifang , He Rui , Shi Rui , Zhang Yiting , Song Shiwei , Su Wei , Liu Houcheng
Published: March 2021

To produce high-quality broccoli microgreens, suitable light intensity for growth and phytochemical contents of broccoli microgreens in an artificial light plant factory were studied. Broccoli microgreens were irradiated under different photosynthetic photon flux density (PPFD): 30, 50, 70 and 90 μmol·m−2·s−1 with red: green: blue = 1:1:1 light-emitting diodes (LEDs). The broccoli microgreens grown under 50 μmol·m−2·s−1 had the highest fresh weight, dry weight, and moisture content, while the phytochemical contents were the lowest. With increasing light intensity, the chlorophyll content increased, whereas the carotenoid content decreased. The contents of soluble protein, soluble sugar, free amino acid, flavonoid, vitamin C, and glucosinolates except for progoitrin in broccoli microgreens were higher under 70 μmol·m−2·s−1. Overall, 50 μmol·m−2·s−1 was the optimal light intensity for enhancement of growth of broccoli microgreens, while 70 μmol·m−2·s−1 was more feasible for improving the phytochemicals of broccoli microgreens in an artificial light plant factory.
Cattle Horn Shavings: A Possible Nitrogen Source for Apple Trees
Authors: Lanauskas Juozas , Uselis Nobertas , Buskienė Loreta , Mažeika Romas , Staugaitis Gediminas , Kviklys Darius
Published: March 2021

The circular economy concept promotes the recycling of agricultural waste. This study was aimed at investigating the effects of cattle horn shavings on apple tree nitrogen nutrition. Ligol apple trees on P 60 rootstock were the object of the study. The experiment was conducted in the experimental orchard of the Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, from 2015 to 2018. Two fertiliser rates were tested: 50 and 100 kg/ha N. Horn shavings (14.1% N) were applied at the end of autumn or at the beginning of vegetation in the spring and in one treatment 100 kg/ha N rate was divided into two equal parts and applied both in autumn and spring. The effects of the horn shavings were compared with the effects of ammonium nitrate (34.4% N) and the unfertilised treatment. The lowest mineral nitrogen content was found in the unfertilised orchard soil and the soil fertilised with horn shavings in the spring at 50 kg/ha N equivalent. In all other cases, the fertilisers increased the soil’s mineral nitrogen content. The lowest leaf nitrogen content was found in apple trees that grew in the unfertilised orchard soil or soil fertilised in the spring with 50 kg/ha N of horn shavings (1.58–2.13%). In other cases, leaf nitrogen content was higher (1.77–2.17%). The apple trees with the lowest leaf nitrogen content produced the smallest average yield (34.5–36.6 t/ha). The highest yield was recorded from fruit trees fertilised with 50 kg/ha N of ammonium nitrate applied in spring or horn shavings applied in autumn (42.4 and 41.4 t/ha, respectively). The influence of horn shavings on the other studied parameters was similar to that of ammonium nitrate. Horn shavings, like nitrogen fertiliser, could facilitate nitrogen nutrition management in apple trees, especially in organic orchards, where the use of synthetic fertilisers is prohibited.
Red Cell Distribution Width Has a Negative Prognostic Role in Dogs with Myxomatous Mitral Valve Disease
Authors: Guglielmini Carlo , Valentini Chiara Martina , Contiero Barbara , Valente Carlotta , Poser Helen
Published: March 2021

Red cell distribution width (RDW) is a quantitative measurement of anisocytosis. This hematological parameter is an important prognostic biomarker for different cardiovascular disorders in humans but its influence on survival has been poorly investigated in dogs with cardiovascular disease. The RDW and various clinical, complete blood count, serum biochemical and echocardiographic variables were retrospectively investigated in 146 client-owned dogs with myxomatous mitral valve disease (MMVD) at various disease stages, with or without concurrent diseases and treatment. Laboratory variables, including RDW, urea, and white blood cell (WBC), in addition to the echocardiographic variable left atrium to aorta ratio were found to be independent predictors of all-cause mortality at six months in a multivariable Cox proportional hazards regression model. In particular, the hazard ratio of RDW was 1.203 (95% confidence interval = 1.045–1.384; p = 0.010). The negative effect of increased RDW on outcome was confirmed using Kaplan–Meier curve analysis. The results of this study indicate that RDW acted as an independent predictor of negative outcome in dogs with MMVD.
Ewe Wastage in New Zealand Commercial Flocks: Extent, Timing, Association with Hogget Reproductive Outcomes and BCS
Authors: Flay Kate J. , Ridler Anne L. , Compton Chris W. R. , Kenyon Paul R.
Published: March 2021

Ewe wastage is the combination of on-farm mortality and premature culling. Internationally, there is limited research on actual wastage incidence and causes in commercial sheep flocks. To the authors’ knowledge, this is the first study that reports both lifetime wastage and detailed annual wastage in a sample of commercial New Zealand flocks. This study utilized data collected from 13,142 ewes from four cohorts on three commercial New Zealand farms (Farm A 2010-born, Farm A 2011-born, Farm B, Farm C), during the period 2011–2017, as they aged from replacement hoggets to 6-year-old ewes (Farm A and Farm B) or 3-year-old ewes (Farm C). Data collection visits occurred at three or four key management times each year, namely pre-mating, pregnancy diagnosis, pre-lambing and weaning. At each visit, body condition score (BCS) was assessed and any ewes that were culled or had died on farm were recorded. As this was a lifetime study, each ewe was assigned an outcome and corresponding ‘exit age’. By the end of the study, all ewes that had exited their respective flocks, were classified as either prematurely culled, or dead/missing, or if still in the flock, as censored, and either the exact date or interval in which they exited the flock was recorded. Semi-parametric competing risk (premature culling vs. dead/missing), interval-censored survival models were developed to: 1. describe the association between hogget reproductive outcomes and risk of subsequent wastage, and 2. assess pre-mating BCS as a predictor of wastage in that production year. Of the 13,142 enrolled ewes, 50.4% exited their respective flocks due to premature culling and 40.0% due to on-farm dead/missing, giving a total of 90.4% that exited due to wastage. Annual mortality incidence ranged from 3.5 to 40.2%. As a hogget, wastage incidence ranged from 7.6 to 45.4%. Pregnancy or rearing a lamb as a hogget did not increase risk of subsequent wastage. In all years, pre-mating BCS was a predictor of ewe wastage, with odds of wastage lower with increasing BCS. Therefore, farmers should focus on improving pre-mating BCS to 3.5/5.0 by assessing ewe BCS at weaning, allowing poorer-BCS ewes to be managed to gain BCS before re-breeding.
The Quality and Health-Promoting Value of Meat from Pigs of the Native Breed as the Effect of Extensive Feeding with Acorns
Authors: Szyndler-Nędza Magdalena , Świątkiewicz Małgorzata , Migdał Łukasz , Migdał Władysław
Published: March 2021

The popularity of meat from animals of native breeds is growing all over the world, due to consumer belief regarding its higher quality compared to meat from industrial farm animals. In addition, the living conditions (welfare) are of great importance for consumers. We observed the effect of different ways of keeping and feeding pigs of the same conservative breed on the quality of meat and its health benefits. The aim of the study was to compare the meat quality from pigs of the native Złotnicka Spotted breed, fattened intensively or extensively (with conventional farm-produced compound feed and acorns). The meat from free-range pigs extensively fed on silage and small amounts of acorns was characterized by a higher content of fat, which acts as a carrier for flavor and juiciness, as well as higher monounsaturated fatty acid (MUFA) content (p ≤ 0.05) and lower atherogenic, thrombogenic, and peroxidability indices (p ≤ 0.05). It may be stated that the meat quality of the native pig breed is significantly dependent on the housing and feeding method. A more beneficial effect on the quality of meat and its dietetic value, as well as its susceptibility to rancidity, can be obtained throughextensive pig feeding with roughage and the addition of acorns.
Adaptive and Biological Responses of Buffalo Granulosa Cells Exposed to Heat Stress under In Vitro Condition
Authors: Faheem Marwa S. , Ghanem Nasser , Gad Ahmed , Procházka Radek , Dessouki Sherif M.
Published: March 2021

The steroidogenesis capacity and adaptive response of follicular granulosa cells (GCs) to heat stress were assessed together with the underlying regulating molecular mechanisms in Egyptian buffalo. In vitro cultured GCs were exposed to heat stress treatments at 39.5, 40.5, or 41.5 °C for the final 24 h of the culture period (7 days), while the control group was kept under normal conditions (37 °C). Comparable viability was observed between the control and heat-treated GCs at 39.5 and 40.5 °C. A higher release of E2, P4 and IGF-1 was observed in the 40.5 °C group compared with the 39.5 or 41.5 °C groups. The total antioxidant capacity was higher in response to heat stress at 39.5 °C. At 40.5 °C, a significant upregulation pattern was found in the expression of the stress resistance transcripts (SOD2 and NFE2L2) and of CPT2. The relative abundance of ATP5F1A was significantly downregulated for all heat-treated groups compared to the control, while TNFα was downregulated in GCs at 39.5 °C. Expression analyses of stress-related miRNAs (miR-1246, miR-181a and miR-27b) exhibited a significant downregulation in the 40.5 °C group compared to the control, whereas miR-708 was upregulated in the 39.5 and 40.5 °C groups. In conclusion, buffalo GCs exhibited different adaptive responses, to the different heat stress conditions. The integration mechanism between the molecular and secretory actions of the GCs cultured at 40.5 °C might provide possible insights into the biological mechanism through which buffalo GCs react to heat stress.
Strain Elastography of Injured Equine Superficial Digital Flexor Tendons: A Reliability Study of Manual Measurements
Authors: Secchi Valentina , Masala Gerolamo , Corda Andrea , Corda Francesca , Potop Enrica , Barbero Fernandez Alicia , Pinna Parpaglia Maria Luisa , Sanna Passino Eraldo
Published: March 2021

Early diagnosis of tendon injuries and accurate long-term monitoring of the healing process are key for equine veterinarians that use conventional ultrasonography. The development of strain elastography could improve the management of clinical cases. The aim of the study was to assess the intraobserver repeatability and interobserver reproducibility of manual measurements of the colored areas of the tendons within elastograms and to standardize this manual modality by comparing the analysis of the images with ImageJ. Twenty elastograms of the injured superficial digital flexor tendons (SDFTs) of horses were analyzed by two different operators after an acute injury was diagnosed with ultrasonography. Statistical analysis demonstrated excellent intraobserver repeatability (intraclass correlation coefficient, ICC = 0.949) and good interobserver reproducibility (ICC = 0.855) for manual measurements performed with tools available on the ultrasound unit. A good agreement between manual measurements and measurements performed with ImageJ (ICC = 0.849) was then demonstrated. Despite its subjectivity, the manual modality proved to be a valid method for analyzing images obtained with strain elastography.
Exploratory Behaviours of Primitive Horses Based on Konik: A Preliminary Study
Authors: Jastrzębska Ewa , Sadowska Joanna , Wnuk-Pawlak Elżbieta , Różańska-Boczula Monika , Janczarek Iwona
Published: March 2021

This study aimed at assessing the behaviour of Konik geldings and mares, kept in a stable and in a free-range system, during behavioural tests regarded as a determinant of the exploration urge. A total of 19 Konik horses kept in individual stables and in a free-range system were included in the study. The experiment was conducted in five phases separated by five-day breaks. A one-stage passive human test was performed during the first phase, a three-stage active human test—2nd phase, a three-stage unknown object test—3rd phase, a two-stage unknown surface test—4th phase, and a one-stage test of social isolation—5th phase. Ten attributes were analysed, including the horse sex and the keeping system. The results were also correlated with one another. Konik horses were found to show the urge to explore, although their behavioural responses are individual and stimulus dependent. In many cases, the horse sex and the keeping system influence the exploratory behaviour, although it is manifested by a greater intensity in geldings than in mares, and in free-range horses than in those kept in a stable. The study is regarded as preliminary due to the small number of horses in the study groups.
Spatio-Temporal Patterns and Consequences of Road Kills: A Review
Authors: Oddone Aquino Ayrton Gino Humberto Emilio , Nkomo S’phumelele Lucky
Published: March 2021

The development and expansion of road networks have profoundly impacted the natural landscape and various life forms. Animals are affected by these roads in a myriad of ways, none as devastating as road mortalities. This article reviews the literature on the magnitude, spatiotemporal patterns, factors, and consequences of Animal-Vehicle Collisions (AVCs) and the subsequent road kills. Furthermore, the review paper briefly outlines the relationship between roads and animals in the surrounding landscape and later examines the nature and impacts of AVCs. This article evaluates the statistics on the number of road kills and a critical analysis of the spatiotemporal patterns of these mortalities is also evaluated. Subsequently, the review paper examines current mitigation measures and the challenges impeding their success. The paper then concludes with an evaluation of geospatial tools (GIS) and other technologies used in road kill studies. The relevant findings of this paper are that, (1) factors influencing road kill patterns interact with one another; (2) AVCs have serious environmental, economic and social consequences; (3) road kill mitigation strategies suffer several challenges hindering their success; and (4) specific geospatial tools and other technologies have been utilised in assessing AVC road kill patterns. The review, therefore, recommends including overall road kill clusters of all animals in mortality surveys, increasing the spatial coverage of road kill observations, consistent surveying, sufficient research on nighttime driving distances and speed, utilising citizen science in all road mortality studies and incorporating GIS into all apps used for recording road kills. An increased sufficiency in road kill data coupled with improved technologies can enable more effective mitigation strategies to prevent AVCs.
Evaluation of the Antimicrobial Activity of Some Components of the Essential Oils of Plants Used in the Traditional Medicine of the Tehuacán-Cuicatlán Valley, Puebla, México
Authors: Candelaria-Dueñas Sebastián , Serrano-Parrales Rocío , Ávila-Romero Marisol , Meraz-Martínez Samuel , Orozco-Martínez Julieta , Ávila-Acevedo José Guillermo , García-Bores Ana María , Cespedes-Acuña Carlos L. , Peñalosa-Castro Ignacio , Hernandez-Delgado Tzasna
Published: March 2021

In Tehuacán-Cuicatlán valley (Mexico), studies have been carried out on the essential oils of medicinal plants with antimicrobial activity and it was found that they present compounds in common such as: α-pinene, β-pinene, carvacrol, eugenol, limonene, myrcene, ocimene, cineole, methyl salicylate, farnesene, and thymol. The goal of this study was to assess the antimicrobial activity of essential oils’ compounds. The qualitative evaluation was carried out by the Kirby Baüer agar diffusion technique in Gram-positive bacteria (11 strains), Gram-negative bacteria (18 strains), and yeasts (8 strains). For the determination of the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), the agar dilution method was used. All the evaluated compounds presented antimicrobial activity. The compounds eugenol and carvacrol showed the largest inhibition zones. Regarding yeasts, the compounds ocimene, cineole, and farnesene did not show any activity. The compounds eugenol, carvacrol, and thymol presented the lowest MIC; bactericidal effect was observed at MIC level for S. aureus 75MR, E. coli 128 MR, and C albicans CUSI, for different compounds, eugenol, carvacrol, and thymol. Finally, this study shows that the essential oils of plants used by the population of Tehuacán-Cuicatlán valley share compounds and some of them have antibacterial and fungicidal activity.
Extra-Nuclear Functions of the Transcription Factor Grainyhead-Like 3 in the Endothelium—Interaction with Endothelial Nitric Oxide Synthase
Authors: Jander Kirsten , Greulich Jan , Gonnissen Stefanie , Ale-Agha Niloofar , Goy Christine , Jakobs Philipp , Farrokh Sabrina , Marziano Corina , Sonkusare Swapnil K. , Haendeler Judith , Altschmied Joachim
Published: March 2021

We previously demonstrated that the transcription factor Grainyhead-like 3 (GRHL3) has essential functions in endothelial cells by inhibiting apoptosis and promoting migration as well as activation of endothelial nitric oxide synthase (eNOS). We now show that a large portion of the protein is localized to myo-endothelial projections of murine arteries suggesting extra-nuclear functions. Therefore, we generated various deletion mutants to identify the nuclear localization signal (NLS) of GRHL3 and assessed potential extra-nuclear functions. Several large-scale deletion mutants were incapable of activating a GRHL3-dependent reporter construct, which could either be due to deficiencies in transcriptional activation or to impaired nuclear import. One of these mutants encompassed a predicted bipartite NLS whose deletion led to the retention of GRHL3 outside the nucleus. Interestingly, this mutant retained functions of the full-length protein as it could still inhibit pathways inducing endothelial cell apoptosis. As apoptosis protection by GRHL3 depends on NO-production, we examined whether GRHL3 could interact with eNOS and showed a direct interaction, which was enhanced with the extra-nuclear GRHL3 variant. The observation that endogenous GRHL3 also interacts with eNOS in intact murine arteries corroborated these findings and substantiated the notion that GRHL3 has important extra-nuclear functions in the endothelium.
Trends in H2S-Donors Chemistry and Their Effects in Cardiovascular Diseases
Authors: Corvino Angela , Frecentese Francesco , Magli Elisa , Perissutti Elisa , Santagada Vincenzo , Scognamiglio Antonia , Caliendo Giuseppe , Fiorino Ferdinando , Severino Beatrice
Published: March 2021

Hydrogen sulfide (H2S) is an endogenous gasotransmitter recently emerged as an important regulatory mediator of numerous human cell functions in health and in disease. In fact, much evidence has suggested that hydrogen sulfide plays a significant role in many physio-pathological processes, such as inflammation, oxidation, neurophysiology, ion channels regulation, cardiovascular protection, endocrine regulation, and tumor progression. Considering the plethora of physiological effects of this gasotransmitter, the protective role of H2S donors in different disease models has been extensively studied. Based on the growing interest in H2S-releasing compounds and their importance as tools for biological and pharmacological studies, this review is an exploration of currently available H2S donors, classifying them by the H2S-releasing-triggered mechanism and highlighting those potentially useful as promising drugs in the treatment of cardiovascular diseases.
Gene and Protein Expression Is Altered by Ascorbate Availability in Murine Macrophages Cultured under Tumour-Like Conditions
Authors: Ang Abel D. , Vissers Margreet C. M. , Burgess Eleanor R. , Currie Margaret J. , Dachs Gabi U.
Published: March 2021

Tumour-associated macrophages (TAMs) are ubiquitously present in tumours and commonly associated with poor prognosis. In immune cells, ascorbate affects epigenetic regulation, differentiation and phenotype via its co-factor activity for the 2-oxoglutarate dependent dioxygenase enzymes. Here, we determined the effect of ascorbate on TAM development in response to tumour microenvironmental cues. Naïve murine bone marrow monocytes were cultured with Lewis Lung Carcinoma conditioned media (LLCM) or macrophage colony-stimulating factor (MCSF) to encourage the development into tumour-associated macrophages. Cells were stimulated with hypoxia (1% O2), with or without ascorbate (500 µM) supplementation. Cells and media were harvested for gene, cell surface marker and protein analyses. LLCM supported bone marrow monocyte growth with >90% of cells staining CD11b+F4/80+, indicative of monocytes/macrophages. LLCM-grown cells showed increased expression of M2-like and TAM genes compared to MCSF-grown cells, which further increased with hypoxia. In LLCM-grown cells, ascorbate supplementation was associated with increased F4/80 cell surface expression, and altered gene expression and protein secretion. Our study shows that ascorbate modifies monocyte phenotype when grown under tumour microenvironmental conditions, but this was not clearly associated with either a pro- or anti-tumour phenotype, and reflects a complex and nuanced response of macrophages to ascorbate. Overall, ascorbate supplementation clearly has molecular consequences for TAMs, but functional and clinical consequences remain unknown.
A Unique Acylated Flavonol Glycoside from Prunus persica (L.) var. Florida Prince: A New Solid Lipid Nanoparticle Cosmeceutical Formulation for Skincare
Authors: Mostafa Eman S. , Maher Ahmed , Mostafa Dalia A. , Gad Sameh S. , Nawwar Mahmoud A.M. , Swilam Noha
Published: March 2021

Polyphenols are known dietary antioxidants. They have recently attracted considerable interest in uses to prevent skin aging and hyperpigmentation resulting from solar UV-irradiation. Prunus persica (L.) leaves are considered by-products and were reported to have a remarkable antioxidant activity due to their high content of polyphenols. This study aimed at the development of a cosmeceutical anti-aging and skin whitening cream preparation using ethanol leaves extract of Prunus persica (L.) (PPEE) loaded in solid lipid nanoparticles (SLNs) to enhance the skin delivery. Chemical investigation of PPEE showed significantly high total phenolic and flavonoids content with notable antioxidant activities (DPPH, ABTS, and β-carotene assays). A unique acylated kaempferol glycoside with a rare structure, kaempferol 3-O-β-4C1-(6″-O-3,4-dihydroxyphenylacetyl glucopyranoside) (KDPAG) was isolated for the first time and its structure fully elucidated. It represents the first example of acylation with 3,4-dihydroxyphenyl acetic acid in flavonoid chemistry. The in-vitro cytotoxicity studies against a human keratinocytes cell line revealed the non-toxicity of PPEE and PPEE-SLNs. Moreover, PPEE, PPEE-SLNs, and KDPAG showed good anti-elastase activity, comparable to that of N-(Methoxysuccinyl)-Ala-Ala-Pro-Val-chloromethyl ketone. Besides, PPEE-SLNs and KDPAG showed significantly (p < 0.001) higher anti-collagenase and anti-tyrosinase activities in comparison to EDTA and kojic acid, respectively. Different PPEE-SLNs cream formulae (2% and 5%) were evaluated for possible anti-wrinkle activity against UV-induced photoaging in a mouse model using a wrinkle scoring method and were shown to offer a highly significant protective effect against UV, as evidenced by tissue biomarkers (SOD) and histopathological studies. Thus, the current study demonstrates that Prunus persica leaf by-products provide an interesting, valuable resource for natural cosmetic ingredients. This provides related data for further studying the potential safe use of PPEE-SLNs in topical anti-aging cosmetic formulations with enhanced skin permeation properties.
Transcriptional Regulation of ROS Homeostasis by the ERR Subfamily of Nuclear Receptors
Authors: Scholtes Charlotte , Giguère Vincent
Published: March 2021

Reactive oxygen species (ROS) such as superoxide anion (O2•−) and hydrogen peroxide (H2O2) are generated endogenously by processes such as mitochondrial oxidative phosphorylation, or they may arise from exogenous sources like bacterial invasion. ROS can be beneficial (oxidative eustress) as signaling molecules but also harmful (oxidative distress) to cells when ROS levels become unregulated in response to physiological, pathological or pharmacological insults. Indeed, abnormal ROS levels have been shown to contribute to the etiology of a wide variety of diseases. Transcriptional control of metabolic genes is a crucial mechanism to coordinate ROS homeostasis. Therefore, a better understanding of how ROS metabolism is regulated by specific transcription factors can contribute to uncovering new therapeutic strategies. A large body of work has positioned the estrogen-related receptors (ERRs), transcription factors belonging to the nuclear receptor superfamily, as not only master regulators of cellular energy metabolism but, most recently, of ROS metabolism. Herein, we will review the role played by the ERRs as transcriptional regulators of ROS generation and antioxidant mechanisms and also as ROS sensors. We will assess how the control of ROS homeostasis by the ERRs can be linked to physiology and disease and the possible contribution of manipulating ERR activity in redox medicine.
Flavonoids’ Effects on Caenorhabditis elegans’ Longevity, Fat Accumulation, Stress Resistance and Gene Modulation Involve mTOR, SKN-1 and DAF-16
Authors: Guerrero-Rubio María Alejandra , Hernández-García Samanta , García-Carmona Francisco , Gandía-Herrero Fernando
Published: March 2021

Flavonoids are potential nutraceutical compounds present in diary food. They are considered health-promoting compounds and promising drugs for different diseases, such as neurological and inflammatory diseases, diabetes and cancer. Therefore, toxicological and mechanistic studies should be done to assert the biological effects and identify the molecular targets of these compounds. In this work we describe the effects of six structurally-related flavonoids—baicalein, chrysin, scutellarein, 6-hydroxyflavone, 6,7-dihydroxyflavone and 7,8-dihydroxyflavone—on Caenorhabditis elegans’ lifespan and stress resistance. The results showed that chrysin, 6-hydroxyflavone and baicalein prolonged C. elegans’ lifespan by up to 8.5%, 11.8% and 18.6%, respectively. The lifespan extensions caused by these flavonoids are dependent on different signaling pathways. The results suggested that chrysin’s effects are dependent on the insulin signaling pathway via DAF-16/FOXO. Baicalein and 6-hydroxyflavone’s effects are dependent on the SKN-1/Nfr2 pathway. In addition, microarray analysis showed that baicalein downregulates important age-related genes, such as mTOR and PARP.
Correct Pronunciation Detection of the Arabic Alphabet Using Deep Learning
Authors: Ziafat Nishmia , Ahmad Hafiz Farooq , Fatima Iram , Zia Muhammad , Alhumam Abdulaziz , Rajpoot Kashif
Published: March 2021

Automatic speech recognition for Arabic has its unique challenges and there has been relatively slow progress in this domain. Specifically, Classic Arabic has received even less research attention. The correct pronunciation of the Arabic alphabet has significant implications on the meaning of words. In this work, we have designed learning models for the Arabic alphabet classification based on the correct pronunciation of an alphabet. The correct pronunciation classification of the Arabic alphabet is a challenging task for the research community. We divide the problem into two steps, firstly we train the model to recognize an alphabet, namely Arabic alphabet classification. Secondly, we train the model to determine its quality of pronunciation, namely Arabic alphabet pronunciation classification. Due to the less availability of audio data of this kind, we had to collect audio data from the experts, and novices for our model’s training. To train these models, we extract pronunciation features from audio data of the Arabic alphabet using mel-spectrogram. We have employed a deep convolution neural network (DCNN), AlexNet with transfer learning, and bidirectional long short-term memory (BLSTM), a type of recurrent neural network (RNN), for the classification of the audio data. For alphabet classification, DCNN, AlexNet, and BLSTM achieve an accuracy of 95.95%, 98.41%, and 88.32%, respectively. For Arabic alphabet pronunciation classification, DCNN, AlexNet, and BLSTM achieve an accuracy of 97.88%, 99.14%, and 77.71%, respectively.
C-Legged Hexapod Robot Design Guidelines Based on Energy Analysis
Authors: Vina Andres , Barrientos Antonio
Published: March 2021

C-legged hexapod robots offer a balanced trade-off between the robust stability of wheeled robots and the increased-motion capabilities of legged robots, and therefore, are currently of great interest. This article investigates the impact of mass, leg radius, and angular velocity on the energy consumption of C-legged hexapod robots, in order to develop a set of design guidelines that maximize the robot’s performance. The kinematic model of a single C-leg system is obtained and used to determine the system’s energy consumption associated with gravitational potential energy (GPE) and kinetic energy (KE) variations. Both the kinematic model and energy model are validated in a custom-made test bench. Our results show that the kinematic model very accurately predicts the trajectory of the system in space, but due to the varying load experienced by the motor, the system lags compared to the model predictions. Furthermore, the energy model has been also validated experimentally and successfully predicts the motor consumption periods. Using the energy model, it has been concluded that the angular velocity of the leg and the leg radius have an exponential relationship with motor peak power demand—directly affecting the motor selection. On the other hand, the mass is inversely proportional to the robot efficiency, and therefore, must be kept as low as possible.
Citizens’ Perception of Smart Cities: A Case Study
Authors: Georgiadis Athanasios , Christodoulou Panayiotis , Zinonos Zinon
Published: March 2021

The 21st century is considered to be “The Century of Cities”. By the end of this century, over 80% of the global population is expected to be living in urban areas. To become smart, a city should develop an approach of services that will focus mainly on citizens to be the primary beneficiaries of the services offered by a Smart City. In this work, we present through a survey of 545 participants, the citizens’ perception about the smart city concept and reveal the Greek and Cypriot citizens’ level of knowledge regards to a Smart City’s actions, applications, and elements. The final results of this study revealed several interesting outcomes. Firstly, this study showed that Cypriot citizens seem to know better what a “Smart City” is compared to Greek citizens, secondly, the study revealed that a large number of participants do not believe that any efforts have been made in their city in order to become “smart” and finally, regards to the most important challenges for the development of a smart city, the survey disclose that the cooperation of the private and public sector is the biggest challenge that needs to be tackled so as citizens can move towards a “smarter” future.
A Single-Shot 3D Measuring Method Based on Quadrature Phase-Shifting Color Composite Grating Projection
Authors: Huang Xiuzhang , Cao Yiping , Yang Chaozhi , Zhang Yujiao , Gao Jie
Published: March 2021

A single-shot three-dimensional measuring method based on quadrature phase-shifting color composite grating projection is proposed. Firstly, three quadrature phase-shifting sinusoidal gratings are encoded in red (R), green (G), and blue (B) channels respectively, composed single- frame color composite grating. This color composite grating is projecting obliquely on the object by DLP. After that, the color camera which is placed in a specific location is used to capture the corresponding color deformed pattern and send it to the PC. Then, by color separation, the color deformed pattern is demodulated as the corresponding three-frame monochromatic deformed patterns with a shifted quadrature phase. Due to the existences of sensitivity differences and color crosstalk among the tricolor channels, we propose a gray imbalance correction method based on the DC component’s consistency approximation. By the established 3D reconstruction physical model, the measurement of 3D shape can be achieved. Many experimental results for static and moving objects prove the proposed method’s feasibility and practicability. Owing to the single-shot feature of the proposed method, it has a good application prospect in real-time and high-speed 3D measurement.
Terahertz Spiral Spatial Filtering Imaging
Authors: Liu Hui , Wu Shiyou , Zhao Meng , Li Chao , Liu XiaoJun , Fang Guangyou
Published: March 2021

In this paper, we propose a terahertz (THz) spiral spatial filtering (SSF) imaging method that can enable image contrast enhancement. The related theory includes three main steps: (1) the THz image of the target is Fourier transformed to the spatial spectrum distribution; (2) the spatial spectrum is modulated by a spiral phase at the Fourier plane; (3) the filtered spatial spectrum is inverse Fourier transformed to the desired THz image. Meanwhile, analytic expression of the final THz image is derived. Due to the unique nature of the spiral phase, THz image contrast enhancement can be achieved and verified by various simulated target images with different contrasts. In our designed THz SSF imaging system, Fourier transform is carried out by the lens, and the spiral phase is acquired by the spiral phase plate (SPP). Proof-of-principle experiments with three different types of targets (carved metal letters, a high-density polyethylene (HDPE) piece with a scratch, and a leaf) were carried out, and the effectiveness of contrast enhancement and edge extraction on the THz reconstruction images was validated.
Evaluation of the Mechanical Properties of Normal Concrete Containing Nano-MgO under Freeze–Thaw Conditions by Evolutionary Intelligence
Authors: Yazdchi Mehdi , Foroughi Asl Ali , Talatahari Siamak , Gandomi Amir H.
Published: March 2021

In this research, different amounts of nano-MgO were added to normal concrete samples, and the effect of these particles on the durability of the samples under freeze and thaw conditions was investigated. The compressive and tensile strength as well as the permeability of concrete containing nanoparticles were measured and compared to those of plain samples (without nanoparticles). The age of concrete samples, percentage of nanoparticles, and water-to-binder ratio are the variables of the current research. Based on the results, the addition of 1% nano-MgO to the normal concrete with a water-to-binder ratio of 0.44 can reduce the permeability up to 63% and improve the compressive and tensile strengths by 9.12% and 10.6%, respectively. Gene Expression Programming (GEP) is applied, and three formulations are derived for the prediction of mechanical properties of concrete containing nano-MgO. In this method, 80% of the dataset is used randomly for the training process and 20% is utilized for testing the formulation. The results obtained by GEP showed acceptable accuracy.
Reliability Analysis of Accelerated Destructive Degradation Testing Data for Bi-Functional DC Motor Systems
Authors: Lee Chinuk , Lim Munwon , Kim Chanjoong , Bae Suk Joo
Published: March 2021

An accelerated degradation test (ADT) has become a popular method to accelerate degradation mechanisms by stressing products beyond their normal use conditions. The components of an automobile are degraded over time or cycle due to their constant exposure to friction or wear. Sometimes, the performance degradation can be measured only by destructive inspection such as operating torques of return-springs in a bi-functional DC motor system. Plastic deformation of the return-spring causes the degradation of actuating forces for shield movement, resulting in deterioration of the shield moving speed in a headlight system. We suggest a step-by-step procedure for a reliability analysis for a bi-functional DC motor in a headlight system, based mainly on accelerated destructive degradation test (ADDT) data. We also propose nonlinear degradation models to describe the ADDT data of the return-springs. Exposure effects of high temperatures on the return-springs are quantitatively modeled through the ADDT models. We compare the estimation results from both the closed-form expression and Monte Carlo simulation to predict the failure–time distribution at normal use conditions, showing that the lifetime estimation results from the closed-form formulation are more conservative.
Analysis of Injected Electron Beam Propagation in a Planar Crossed-Field Gap
Authors: Bhattacharya Ranajoy , Darr Adam M. , Garner Allen L. , Browning Jim
Published: March 2021

This paper examines basic crossed-field device physics in a planar configuration, specifically electron beam perturbation and instability as a function of variation in magnetic field, and angle between magnetic and electric field. We perform a three-dimensional (3-D) simulation of electron perturbation in a planar crossed-field system using the full 3-D particle trajectory solver in CST Particle Studio (CST-PS). The structure has a length, height, width and anode-sole gap of 15 cm, 2 cm, 10 cm, and 2 cm, respectively. The anode to sole voltage is fixed at 3 kV, and the magnetic field and injected current varied from 0.01 T to 0.05 T and 1.5 mA to 1 A, respectively. The simulations show that applying a magnetic field of 0.05 T makes the beam stable for a critical current density of 94 mA/cm2 for an anode-sole gap of 20 mm. Above this current density, the beam was unstable, as predicted. Introducing a 1° tilt in the magnetic field destabilizes the beam at a current density of 23 mA/cm2, which is lower than the critical current density for no tilt, as predicted by our theory. The simulation results also agree well with prior one-dimensional (1-D) theory and simulations that predict stable bands of current density for a 5° tilt where the beam is stable at low current density (<13.3 mA/cm2), unstable above this threshold, and then stable again at higher current density, (>33 mA/cm2).
Numerical Measurement of a Virtual Model for the Knee Prosthesis Geometry
Authors: Zehouani Kheireddine , István Oldal
Published: March 2021

The human knee joint usually suffers progressive deterioration with time. The conventional cure of this issue is to replace it with an alternate knee by applying the prosthesis implant. The reason is that the process causes the abrasion of the different materials rather than just sliding or rolling. This study aims to develop the numerical measurement of the knee prosthesis’s geometry, which fulfils the mechanical requirements of the human knee. The MSC.ADAMS programme was applied to demonstrate the movement of the human knee joint in terms of rotation and flexion. The changes between the condyles of the developed multibody of the prosthesis related to the flexion angle ranging from 20–120° were investigated and presented. The boundary conditions were determined, and simulations performed using the ADAM’s programme. An average value of 0.7 was reached for the slip ration, with the maximum getting up to 0.79. An angle between 110–120° for the flexion angle was obtained. It can be said that the application of the multibody model saves time as there is no involvement of the tibia and the femur as required for the knee prosthesis. More importantly, as the application of the test machine is omitted in our process, our model’s approximations to a human knee are carried out directly. Without cost, several measurements for the knee prosthesis could be made and repaired. The study results provide the necessary insight for future tests regarding the movement of the knee joint.
Compensation for In-Phase/Quadrature Phase Mismatch in Coherent Free-Space Optical QPSK Communication Systems
Authors: Li Xueliang , Geng Tianwen , Gu Yucong , Tian Ruotong , Gao Shijie
Published: March 2021

The Gram–Schmidt orthogonalization procedure (GSOP) and Löwdin symmetric orthogonalization procedure (SYOP) are the two mainstream algorithms for the compensation of phase mismatch in an imperfect optical 90° hybrid. In this paper, we put forward an algorithm switching orthogonalization procedure (ASOP) according to the quality of in-phase and quadrature signals based on the Q value of the eye diagram with less computation. If the quality of the in-phase and quadrature signals has a significant difference, we use the GSOP and select the signal branch with better quality as the initial reference vector for orthogonalization. If they are of about the same quality, then we use the SYOP. We present computer simulations for a coherent free-space optical (FSO) quadrature phase-shift keying (QPSK) communication system and demonstrate the system improvement that can be achieved using the ASOP. Finally, we also show that the proposed ASOP scheme can contribute to the frequency offset and phase estimation of the FSO system in the environment of atmospheric turbulence.
Effects of Wildfire on Rockfall Occurrence: A Review through Actual Cases in Spain
Authors: Sarro Roberto , Pérez-Rey Ignacio , Tomás Roberto , Alejano Leandro R. , Hernández-Gutiérrez Luis Enrique , Mateos Rosa María
Published: March 2021

Understanding processes and conditions that lead to rockfalls during and after a wildfire in different geological contexts is crucial since this phenomenon is one of the major hazards in mountainous regions across Europe. Spain is one of the European countries with the highest rate of wildfires, and rockfalls cause high economic and social impact, with many fatalities every year. The increase of rockfalls during and after wildfires is connected with the merging of different factors, not only in the detached area but also in the propagation and potentially affected area. When wildfire occurred, many actions take place: changes in the mechanical conditions of the rocks, the loss of protective capacity from vegetation, the effect induced by firefighting activities and/or the impact by the high temperatures in the adopted protective measures. After the wildfire, there is an increase in frequency and intensity of rockfalls in the burned area, causing a major impact of rockfalls on not only road networks and built-up areas but also people living. Additionally, the removal of vegetation by wildfires causes an increase in the risk perception, related not only to detached blocks but also to the general appearance of the rock mass. In this review, the main factors that influence the occurrence of rockfalls after a wildfire are analyzed, and three actual case studies in Spain are presented to support the variety of conclusions obtained.
A Numerical Investigation of Laser Beam Welding of Stainless Steel Sheets with a Gap
Authors: Buttazzoni Michele , Zenz Constantin , Otto Andreas , Gómez Vázquez Rodrigo , Liedl Gerhard , Arias Jorge Luis
Published: March 2021

Keyhole laser beam welding (LBW) of 304L stainless steel sheets with a gap in between was numerically simulated with a three-dimensional, transient, multi-physical model for laser material processing based on the finite volume method (FVM). First, the model’s ability to reproduce experimental results on a relatively coarse computational mesh within reasonable computing time, so as to serve as process optimization tool, is presented. An example of process optimization is shown, wherein a given set of weld seam quality criteria is fulfilled by iteratively optimizing a secondary laser beam. The relatively coarse mesh, in combination with a good model calibration for the experimental conditions, allows for sufficiently fast simulations to use this approach for optimization tasks. Finally, using a finer spatial and temporal discretization, the dynamic processes in the vicinity of the keyhole leading to the formation of pores are investigated. The physical phenomena predicted by the simulation are coherent with experimental observations found in literature.
Determination of the Qualitative Composition of Biologically Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhaponticum carthamoides
Authors: Asyakina Lyudmila , Ivanova Svetlana , Prosekov Alexander , Dyshlyuk Lyubov , Chupakhin Evgeny , Ulrikh Elena , Babich Olga , Sukhikh Stanislav
Published: March 2021

This work aims to study the qualitative composition of biologically active substance (BAS) extracts in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides. The research methodology is based on high-performance liquid chromatography, and 1H nuclear magnetic resonance (NMR) spectra, to study the qualitative and quantitative analysis of BAS. The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension and root cultures showed that the main biologically active substances in the medicinal plant Rhaponticum carthamoides are 2-deoxy-5,20,26-trihydroxyecdyson (7 mg, yield 0.12%), 5,20,26-trihydroxyecdyson 20,22-acetonide (15 mg, yield 0.25%), 2-deoxy-5,20,26-trihydroxyecdyson 20,22-acetonide (6 mg, yield 0.10%), 20,26-dihydroxyecdyson 20,22-acetonidecdyson 20,22-acetonide (5 mg, yield 0.09%), and ecdyson 20,22-acetonide (6 mg, yield 0.10%). In the future, it is planned to study the antimicrobial, antioxidant, and antitumor activity of BAS of extracts of in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides, for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial and antioxidant effects.