bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of EST-SSR Markers Related to Polyphyllin Biosynthesis Reveals Genetic Diversity and Population Structure in Paris polyphylla
Authors: Gao Xiaoyang , Su Qixuan , Yao Baolin , Yang Wenjing , Ma Weisi , Yang Bin , Liu Changning
Published: July 2022

Paris polyphylla is an important medicinal plant that can biosynthesize polyphyllins with multiple effective therapies, ranging from anti-inflammation to antitumor; however, the genetic diversity of Paris polyphylla is still unclear. To explore the genetic characteristics of cultivation populations in primary planting areas, we developed 10 expressed sequence tag simple sequence repeat (EST-SSR) markers related to polyphyllin backbone biosynthesis and utilized them in 136 individuals from 10 cultivated populations of P. polyphylla var. yunnanensis. The genetic diversity index showed that ten loci had relatively high genetic polymorphism levels. Shannon information of loci suggested that more information occurred within population and less information occurred among population. In addition, the overall populations exhibited a low degree of differentiation among populations, but maintained a high degree of genetic diversity among individuals, resulting in high gene flow and general hybridization. The genetic structure analysis revealed that 10 populations possibly derived from two ancestral groups and all individuals were found with different levels of admixture. The two groups were different from the cultivation groups at population level, suggesting the cross-pollination among cultivars. These findings will provide insights into the genetic diversity of the germplasm resources and facilitate marker-assisted breeding for this medicinal herb.
Wolves, Crows, Spiders, and People: A Qualitative Study Yielding a Three-Layer Framework for Understanding Human–Wildlife Relations
Authors: Jürgens Uta M. , Hackett Paul M. W. , Hunziker Marcel , Patt Anthony
Published: July 2022

Human dimensions research has proposed a multitude of variables impacting the viability of wildlife populations. Extant approaches to systematizing these variables have mostly focused on human relations to only one animal species or taxon and are largely descriptive, rather than explanatory. In this study, we provide a three-layer framework for understanding people’s responses to a variety of human–wildlife encounters. We conducted a comparative qualitative study, interviewing 20 stakeholders on one of three ecologically disparate model animals. Through thematic analysis, we identified person-specific, species-specific, and overarching factors whose interplay shapes people’s reactions to encounters with wildlife. The person-specific factors, individual people’s biographic backgrounds and life themes, fuel the polarization of stances towards wildlife. The species-specific factors, people’s mental images of wild animals, explain the particular character of different human–wildlife relations. The overarching factors, fundamental questions regarding the place of humans in nature or motivations of control over animal agents, stir the intensity inherent in human encounters with wildlife. This three-layer framework amends existing proposals by providing a cohesive system and an in-depth portrayal of shared and specific factors and processes in various human–wildlife relations and by elucidating their interaction in influencing people’s responses to encounters with wild animals.
The Change in Microbial Diversity and Mycotoxins Concentration in Corn Silage after Addition of Silage Additives
Authors: Kalúzová Mária , Kačániová Miroslava , Bíro Daniel , Šimko Milan , Gálik Branislav , Rolinec Michal , Hanušovský Ondrej , Felšöciová Soňa , Juráček Miroslav
Published: July 2022

In our study the presence of bacteria, yeast, and microscopic fungi was evaluated. Three forms of corn silage were made including silage without additive, silage with microbial additive (lactic acid bacteria), and silage with nutritional additive (urea). Silage additives were applied to the matter within the recommended dosage, then the matter was ensiled into plastic bags and stored at a constant temperature. After 5.5 months of storage, average samples for microbial and mycotoxins analysis were taken. From microbiological points, the plate count agar method for enumeration of total count of bacteria, lactic acid bacteria, enterococci, yeasts, and microscopic fungi and mass spectrometry for microbiota identification were used. In total, 43 species of bacteria and yeasts and 6 genera of microscopic fungi were identified from all samples of corn silages. The most isolated species were Lentilactobacillus buchneri and Kazachstania exigua from bacteria resp. yeasts and Aspergillus and Penicillium from microscopic fungi. Mycotoxins were determined by HPLC-MS/MS and divided into two groups as regulated and emerging. In the corn silages only Fusarium mycotoxins were observed. All corn silages, regardless of the addition of the additive, were the highest in nivalenol content. Deoxynivalenol and beauvericin with the highest concentrations were present in silage with urea. Although the mycotoxins content of the variants changed, these changes were not statistically significant. In general, addition of lactic acid bacteria Lentilactobacillus buchneri and Lacticaseibacillus casei and urea as silage additives affect the microbial diversity; however, the hygienic quality of whole crop corn silage was not negatively changed.
Seasonal Variations of Milk Composition of Sarda and Saanen Dairy Goats
Authors: Scano Paola , Caboni Pierluigi
Published: July 2022

Traditionally, in Mediterranean areas the goat population was composed of autochthonous breeds with strong milk production seasonality. In the last decades, high productive alpine breeds were introduced together with more widespread out-of-season milk production practices. This study is a large-scale survey on the seasonal variations of the main compositional characteristics of goat milk obtained from Sarda and Saanen breeds reared on the Mediterranean island of Sardinia (Italy). Analysis of data indicated that milk from the Sarda breed was significantly richer, at p < 0.001, in protein, fat, and lactose, and had a lower urea mean content than Saanen. Throughout the year, fluctuations of mean contents of the milk parameters were similar for the two groups of goats, indicating that, besides genetic intrinsic differences, climate and herbage growth influenced the Sarda as well as the Saanen goats. During the summer, milk from Saanen showed a marked drop in fat and protein contents, with 21% of samples showing a fat-to-protein ratio <1. No significant differences were found for the somatic cell count; however, the Sarda breed showed a higher bacterial count, suggesting improper milk handling and/or storage equipment more frequently encountered in extensive and semi-extensive farm systems.
Quantum Thermodynamic Uncertainty Relations, Generalized Current Fluctuations and Nonequilibrium Fluctuation–Dissipation Inequalities
Authors: Reiche Daniel , Hsiang Jen-Tsung , Hu Bei-Lok
Published: July 2022

Thermodynamic uncertainty relations (TURs) represent one of the few broad-based and fundamental relations in our toolbox for tackling the thermodynamics of nonequilibrium systems. One form of TUR quantifies the minimal energetic cost of achieving a certain precision in determining a nonequilibrium current. In this initial stage of our research program, our goal is to provide the quantum theoretical basis of TURs using microphysics models of linear open quantum systems where it is possible to obtain exact solutions. In paper [Dong et al., Entropy 2022, 24, 870], we show how TURs are rooted in the quantum uncertainty principles and the fluctuation–dissipation inequalities (FDI) under fully nonequilibrium conditions. In this paper, we shift our attention from the quantum basis to the thermal manifests. Using a microscopic model for the bath’s spectral density in quantum Brownian motion studies, we formulate a “thermal” FDI in the quantum nonequilibrium dynamics which is valid at high temperatures. This brings the quantum TURs we derive here to the classical domain and can thus be compared with some popular forms of TURs. In the thermal-energy-dominated regimes, our FDIs provide better estimates on the uncertainty of thermodynamic quantities. Our treatment includes full back-action from the environment onto the system. As a concrete example of the generalized current, we examine the energy flux or power entering the Brownian particle and find an exact expression of the corresponding current–current correlations. In so doing, we show that the statistical properties of the bath and the causality of the system+bath interaction both enter into the TURs obeyed by the thermodynamic quantities.
Complex Network Analysis of Mass Violation, Specifically Mass Killing
Authors: Erum Iqra , Malick Rauf Ahmed Shams , Ahmed Ghufran , Cherifi Hocine
Published: July 2022

News reports in media contain news about society’s social and political conditions. With the help of publicly available digital datasets of events, it is possible to study a complex network of mass violations, i.e., Mass Killings. Multiple approaches have been applied to bring essential insights into the events and involved actors. Power law distribution behavior finds in the tail of actor mention, co-actor mention, and actor degree tells us about the dominant behavior of influential actors that grows their network with time. The United States, France, Israel, and a few other countries have been identified as major players in the propagation of Mass Killing throughout the past 20 years. It is demonstrated that targeting the removal of influential actors may stop the spreading of such conflicting events and help policymakers and organizations. This paper aims to identify and formulate the conflicts with the actor’s perspective at a global level for a period of time. This process is a generalization to be applied to any level of news, i.e., it is not restricted to only the global level.
An Optimal WSN Node Coverage Based on Enhanced Archimedes Optimization Algorithm
Authors: Dao Thi-Kien , Chu Shu-Chuan , Nguyen Trong-The , Nguyen Trinh-Dong , Nguyen Vinh-Tiep
Published: July 2022

Node coverage is one of the crucial metrics for wireless sensor networks’ (WSNs’) quality of service, directly affecting the target monitoring area’s monitoring capacity. Pursuit of the optimal node coverage encounters increasing difficulties because of the limited computational power of individual nodes, the scale of the network, and the operating environment’s complexity and constant change. This paper proposes a solution to the optimal node coverage of unbalanced WSN distribution during random deployment based on an enhanced Archimedes optimization algorithm (EAOA). The best findings for network coverage from several sub-areas are combined using the EAOA. In order to address the shortcomings of the original Archimedes optimization algorithm (AOA) in handling complicated scenarios, we suggest an EAOA based on the AOA by adapting its equations with reverse learning and multidirection techniques. The obtained results from testing the benchmark function and the optimal WSN node coverage of the EAOA are compared with the other algorithms in the literature. The results show that the EAOA algorithm performs effectively, increasing the feasible range and convergence speed.
A Comparison of Partial Information Decompositions Using Data from Real and Simulated Layer 5b Pyramidal Cells
Authors: Kay Jim , Schulz Jan , Phillips William
Published: July 2022

Partial information decomposition allows the joint mutual information between an output and a set of inputs to be divided into components that are synergistic or shared or unique to each input. We consider five different decompositions and compare their results using data from layer 5b pyramidal cells in two different studies. The first study was on the amplification of somatic action potential output by apical dendritic input and its regulation by dendritic inhibition. We find that two of the decompositions produce much larger estimates of synergy and shared information than the others, as well as large levels of unique misinformation. When within-neuron differences in the components are examined, the five methods produce more similar results for all but the shared information component, for which two methods produce a different statistical conclusion from the others. There are some differences in the expression of unique information asymmetry among the methods. It is significantly larger, on average, under dendritic inhibition. Three of the methods support a previous conclusion that apical amplification is reduced by dendritic inhibition. The second study used a detailed compartmental model to produce action potentials for many combinations of the numbers of basal and apical synaptic inputs. Decompositions of the entire data set produce similar differences to those in the first study. Two analyses of decompositions are conducted on subsets of the data. In the first, the decompositions reveal a bifurcation in unique information asymmetry. For three of the methods, this suggests that apical drive switches to basal drive as the strength of the basal input increases, while the other two show changing mixtures of information and misinformation. Decompositions produced using the second set of subsets show that all five decompositions provide support for properties of cooperative context-sensitivity—to varying extents.
Regional Specialization, Competitive Pressure, and Cooperation: The Cocktail for Innovation
Authors: Marco-Lajara Bartolomé , Sánchez-García Eduardo , Martínez-Falcó Javier , Poveda-Pareja Esther
Published: July 2022

The main aim of this paper is to analyze the effect of industrial agglomeration on the degree of interorganizational cooperation and the innovative performance of firms of the electricity supply sector in Spain. For this purpose, the agglomeration coefficient in each of the 50 provinces of Spain is calculated, based on secondary data from SABI database. Subsequently, primary data are obtained from a sample of 197 companies through a structured questionnaire. In this case, the PLS-SEM technique is used. The results show that there is a positive and significant relationship between the variables analyzed. It is concluded that industrial agglomeration and cooperation are relevant external factors that boost the innovative performance of firms and that business associations foster interorganizational cooperation.
Low-Cost I–V Tracer for PV Fault Diagnosis Using Single-Diode Model Parameters and I–V Curve Characteristics
Authors: Kongphet Vorachack , Migan-Dubois Anne , Delpha Claude , Lechenadec Jean-Yves , Diallo Demba
Published: July 2022

The continuous health monitoring of PV modules is mandatory to maintain their high efficiency and minimize power losses due to faults or failures. In this work, a low-cost embedded tracer is developed to measure the I–V curve of a PV module in less than 0.2 s. The data are used to extract the five parameters of the single-diode model and its main characteristics (open-circuit voltage, short-circuit current, and maximum power). Experimental data are used to validate the analytical model and evaluate the two fault diagnosis methods, using as fault features the parameters of the single-diode model or the main characteristics of the I–V curve. The results, based on field data under different temperatures and irradiances, show that the degradation of series and shunt resistances could be detected more accurately with the main characteristics rather than with the parameters. However, the estimated parameters could still be used to monitor the long-term degradation effects.
Investigation of Energy-Saving Strategy for Parallel Variable Frequency Pump System Based on Improved Differential Evolution Algorithm
Authors: Qin Xuecong , Luo Yin , Chen Shengyuan , Chen Yunfei , Han Yuejiang
Published: July 2022

This paper presents an energy-saving strategy that was applied to a parallel variable frequency pump system of a water circulation pumping station. Firstly, the mathematical model of shaft power consumption for the parallel pump system was established using quadratic polynomial fitting, with some constraints configured according to the system’s water supply demands. Then, the algorithm program was designed with the goal of minimizing the energy consumption through the application of an improved differential evolution algorithm. Additionally, the energy consumption model and constraints were integrated and simplified in order to adapt to the algorithm calculation. In the end, the algorithm was implemented according to the pump design parameters and supply targets of the pumping station. Meanwhile, a comparison was done between the differential evolution (DE) algorithm and the genetic algorithm (GA). Furthermore, an experimental test was conducted in an aluminum company in order to verify the applicability of the energy-saving algorithm in practice. The results demonstrated the feasibility of using the improved differential evolution algorithm in order to achieve a minimum energy consumption operation strategy; the results consequently manifested the superiority of the differential evolution algorithm in both computing time and optimal solution aspects.
A Hybrid Three-Level ZVZCS Converter for Photovoltaic Power Connecting to MVDC Collection System
Authors: He Xiaokun , Hu Renjie , Chen Wu
Published: July 2022

Compared with the medium voltage ac (MVAC) collection system, the medium voltage dc (MVDC) one for renewable energy sources has many advantages. High-power dc/dc converters are one of the key stages of the MVDC collection system to boost the voltage generated by photovoltaic or wind turbine. A novel hybrid three-level dc/dc converter utilizing a blocking capacitor to realize zero-voltage zero-current-switching (ZVZCS) is proposed. A higher overall efficiency can be achieved by reducing conduction losses. Detailed experimental results on a scaled-down hardware prototype rated at 150 V/750 V/1 kW are demonstrated to verify the proposed converter performance.
Effective Methods for Adventitious Root Regeneration on Weeping Fig Stems
Authors: Li Nelson , Chang Yusen
Published: July 2022

When transplanting mature Ficus trees, the large root balls are expensive to treat, handle, and move. This study aimed to identify the optimal wounding method and auxin treatment for regenerating adventitious roots (ARs) from weeping fig (Ficus benjamina L.) stems to uptake additional water and to compensate for fewer absorption roots in the smaller root balls at transplantation. We adopted a two-factorial experiment involving the wounding methods (three-line cut (3LC) and rectangular peel (RP)) and auxin treatments (2000 mg·L−1 Indole-3-butyric acid (IBA), 2000 mg·L−1 IBA + 2000 mg·L−1 1-naphthaleneacetic acid (NAA), and 4000 mg·L−1 IBA). The rooting rate of each treatment, the mean root number, the length of the three longest ARs, and the dry weight of ARs in each wound were evaluated. The treatment combination using 4000 mg·L−1 IBA with RP13 (rectangular peel 1/3 the perimeter of the stem) consistently exhibited the best rooting results in 2019 and 2020. It had a 100% rooting rate, a mean of 18.5 roots, a 16.8 cm root length, and a 1640 mg dry weight in the wounds. All auxin treatments demonstrated a superior rooting ability as compared to water treatments. The RP method regenerated more roots than the 3LC method. Doubling the RP length to be 2/3 of the perimeter improved the rooting ability. The locations of ARs varied under different treatment combinations, with 4000 mg·L−1 IBA on RP13 demonstrating the most diversified distribution on four edges of the wounds. Thus, it is recommended to regenerate ARs from stems of F. benjamina trees.
Nitrogen Addition Decreases Rhizodeposition by Chinese Fir (Cunninghamia lanceolata (Lamb.) Hook) Seedlings and Its Distribution in Soil Aggregates
Authors: Chen Bohan , Wang Jiao , Duan Xuan , Zhao Fengxia , Zhang Weidong , Guan Xin , Chen Longchi , Wang Qingkui , Wang Silong , Yang Qingpeng
Published: July 2022

Rhizodeposition-derived carbon plays an important role in plant nutrient acquisition and soil carbon sequestration. However, how nitrogen deposition affects the distribution of rhizodeposition-derived carbon into aggregate classes (macrogagregates, microaggregates, and silt and clay) is unclear. We conducted a nitrogen addition experiment on Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) seedlings with continuously labeled 13CO2 for 120 days. Plant growth and the distribution of rhizodeposition-derived carbon into aggregate classes were assessed. Results showed that nitrogen additionconsiderably increased the ratio of aboveground to belowground biomass, but not aboveground and belowground biomass. Compared with the control, nitrogen addition resulted in a significantdecreaseby 52% inrhizodeposition-derived carbon in bulk soil.We found that more rhizodeposition-derived carbon was incorporated into macroaggregate, followed by microaggregate, and silt and clay regardless of nitrogen addition. The rhizodeposition-derived carbon was significantly decreased by 40% in macroaggregate, 60% in microaggregate, and 61% in silt and clay after nitrogen addition. Nitrogen addition and aggregate classes had no interactive effect on the rhizodeposition-derived carbon. Our results suggest that nitrogen deposition decreases the rhizodeposition of Chinese fir and its distributionin aggregate classes.
Compressive Strength Characteristic Values of Nine Structural Sized Malaysian Tropical Hardwoods
Authors: Azmi Anis , Ahmad Zakiah , Lum Wei Chen , Baharin Adnie , Za’ba Nurul Izzatul Lydia , Bhkari Norshariza Mohamad , Lee Seng Hua
Published: July 2022

The design practice of timber structures in Malaysia is still based on permissible stress codes as stated in Malaysian Standard (MS) 544: Part 2 and MS 544: Part 3, which was adopted from the British Standard (BS) 5268. The British Standard was later completely replaced by Eurocode 5 (EC5) in 2009. Therefore, to preserve the continuity of design concepts specified in the British code of practice, local designers should adopt an EC5 limit state design to generate safe and economical designs. However, new strength data based on characteristic values which comply with EC5 for Malaysian tropical hardwoods are still lacking. The aim of this study was to investigate the compressive strength properties of nine structural-sized Malaysian tropical hardwood species namely Balau, Kempas, Kelat, Resak, Kapur, Keruing, Mengkulang, Light Red Meranti and Geronggang tested according to European Standard (EN) 408. A compression test was performed to measure the compressive strength and modulus of elasticity of the timbers and were used to derive characteristic values. The equation for determining characteristic compressive strength given in EN 384 was also assessed to verify that whether it is suitable for high density Malaysian hardwoods, as this equation was derived from softwood and European hardwoods. The results revealed that the derived characteristic values are higher than the values given in EN 338 for the relevant strength classes, particularly for heavy and medium hardwood with densities greater than 700 kg/m3. A verification of the equation used in EN 384 to determine compressive strength characteristic value yields a different equation, fc,0,k=2.2 fm,k0.7. This shows that the EN 384 equation is not suitable to be used with hardwood timber with a density more than 700 kg/m3, since it will underestimate the strength value.
The Gender-Specific Association of DRD2 Polymorphism with Metabolic Syndrome in Patients with Schizophrenia
Authors: Paderina Diana Z. , Boiko Anastasiia S. , Pozhidaev Ivan V. , Mednova Irina A. , Goncharova Anastasia A. , Bocharova Anna V. , Fedorenko Olga Yu. , Kornetova Elena G. , Semke Arkadiy V. , Bokhan Nikolay A. , Loonen Anton J. M. , Ivanova Svetlana A.
Published: July 2022

Background: Metabolic syndrome is widespread in patients with schizophrenia receiving long-term antipsychotic therapy. Dopamine D2 receptors play an important role in mediating both the therapeutic actions of antipsychotics and their side effects. The present study examined the association of two polymorphisms of the DRD2 gene with metabolic syndrome in patients with schizophrenia. Methods: We examined 517 patients from several regions of Siberia (Russia) with a clinical diagnosis of schizophrenia. Genotyping of two single nucleotide polymorphisms rs1799732 and rs4436578 of the dopamine D2 receptor gene (DRD2) was performed in a population of 471 patients. The results were analyzed using chi-square tests. Results: Functional polymorphism rs1799732 of the DRD2 gene is associated with drug-induced metabolic syndrome in women with schizophrenia. Conclusions: Our results show that the DRD2 gene may be involved in the pathogenesis of metabolic disorders in patients with schizophrenia. Further analysis of possible genetic markers will allow for personalized treatment with minimal side effects and optimal efficacy. This which seems relevant in light of the recent focus on improving the quality of life and ensuring a high level of social adaptation of patients with schizophrenia.
Phenotypic Variation in Two Siblings Affected with Shwachman-Diamond Syndrome: The Use of Expert Variant Interpreter (eVai) Suggests Clinical Relevance of a Variant in the KMT2A Gene
Authors: Taha Ibrahim , De Paoli Federica , Foroni Selena , Zucca Susanna , Limongelli Ivan , Cipolli Marco , Danesino Cesare , Ramenghi Ugo , Minelli Antonella
Published: July 2022

Introduction. Shwachman-Diamond Syndrome (SDS) is an autosomal-recessive disorder characterized by neutropenia, pancreatic exocrine insufficiency, skeletal dysplasia, and an increased risk for leukemic transformation. Biallelic mutations in the SBDS gene have been found in about 90% of patients. The clinical spectrum of SDS in patients is wide, and variability has been noticed between different patients, siblings, and even within the same patient over time. Herein, we present two SDS siblings (UPN42 and UPN43) carrying the same SBDS mutations and showing relevant differences in their phenotypic presentation. Study aim. We attempted to understand whether other germline variants, in addition to SBDS, could explain some of the clinical variability noticed between the siblings. Methods. Whole-exome sequencing (WES) was performed. Human Phenotype Ontology (HPO) terms were defined for each patient, and the WES data were analyzed using the eVai and DIVAs platforms. Results. In UPN43, we found and confirmed, using Sanger sequencing, a novel de novo variant (c.10663G > A, p.Gly3555Ser) in the KMT2A gene that is associated with autosomal-dominant Wiedemann–Steiner Syndrome. The variant is classified as pathogenic according to different in silico prediction tools. Interestingly, it was found to be related to some of the HPO terms that describe UPN43. Conclusions. We postulate that the KMT2A variant found in UPN43 has a concomitant and co-occurring clinical effect, in addition to SBDS mutation. This dual molecular effect, supported by in silico prediction, could help to understand some of the clinical variations found among the siblings. In the future, these new data are likely to be useful for personalized medicine and therapy for selected cases.
Genetic Polymorphism at 15 Codons of the Prion Protein Gene in 156 Goats from Romania
Authors: Gurau Maria Rodica , Negru Elena , Ionescu Teodor , Udriste Anca Amalia , Cornea Călina Petruța , Baraitareanu Stelian
Published: July 2022

Background: The variability of prion protein gene (PRNP) codons and the frequency of alleles (K222, D146, and S146) that appear to confer genetic resistance to classical scrapie are still unknown in several goat populations/breeds prevalent in Romania. This work aims to assess the genetic polymorphism at 15 PRNP codons in Romanian goat populations to inform the development of goat breeding programs for scrapie resistance. Methods: Whole blood and hair follicles from Carpathian (50), French Alpine (53), and Banat’s White (53) breed goats were sampled to extract genomic DNA for genetic analyses and Sanger sequencing. In the targeted goat groups, one classical scrapie-positive Banat’s White goat was included. Results: The codons without polymorphisms were G37G, W102W, N146N, R151R, S173S, and I218I. The following non-synonymous polymorphisms of PRNP were recorded: P110P, P110S, P110T, T110T, G127G, G127S, I142I, I142M, T142I, H143H, P143P, R143R, R154R, H154R, P168P, Q168Q, Q211Q, Q211R, Q222Q, H222Q, K222K, S240S, P240P, P240S, and S240P. Conclusions: PRNP polymorphism was recorded in 60% (9/15) of codons. The scrapie-positive Banat’s White goat had G37G, W102W, T110T, G127G, I142I, H143H, N146N, R151R, R154R, P168P, S173S, R211R, I218I, Q222Q, and S240S. The K222 allele had a frequency of 6% (3/50) in Carpathian, 9.43% (5/53) in Banat’s White, and 15.09% (8/53) in French Alpine. Therefore, the polymorphisms detected in this sample of Romanian goat breeds are too rare to design a breeding program at the current time.
Cadmium Ecotoxic Effects on Embryonic Dmrt1 and Aromatase Expression in Chrysemys picta Turtles May Implicate Changes in DNA Methylation
Authors: Mizoguchi Beatriz , Topping Nicholas E. , Lavin Andrew M. , Valenzuela Nicole
Published: July 2022

Temperature-dependent sex determination (TSD) decides the sex fate of an individual based on incubation temperature. However, other environmental factors, such as pollutants, could derail TSD sexual development. Cadmium is one such contaminant of soils and water bodies known to affect DNA methylation, an epigenetic DNA modification with a key role in sexual development of TSD vertebrate embryos. Yet, whether cadmium alters DNA methylation of genes underlying gonadal formation in turtles remains unknown. Here, we investigated the effects of cadmium on the expression of two gene regulators of TSD in the painted turtle, Chrysemys picta, incubated at male-producing and female-producing temperatures using qPCR. Results revealed that cadmium alters transcription of Dmrt1 and aromatase, overriding the normal thermal effects during embryogenesis, which could potentially disrupt the sexual development of TSD turtles. Results from a preliminary DNA methylation-sensitive PCR assay implicate changes in DNA methylation of Dmrt1 as a potential cause that requires further testing (aromatase methylation assays were precluded).
The Role of Cytotoxic T-Lymphocyte Antigen 4 in the Pathogenesis of Multiple Sclerosis
Authors: Basile Maria Sofia , Bramanti Placido , Mazzon Emanuela
Published: July 2022

Multiple sclerosis (MS) is an autoimmune neurodegenerative disorder of the central nervous system that presents heterogeneous clinical manifestations and course. It has been shown that different immune checkpoints, including Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), can be involved in the pathogenesis of MS. CTLA-4 is a critical regulator of T-cell homeostasis and self-tolerance and represents a key inhibitor of autoimmunity. In this scopingreview, we resume the current preclinical and clinical studies investigating the role of CTLA-4 in MS with different approaches. While some of these studies assessed the expression levels of CTLA-4 on T cells by comparing MS patients with healthy controls, others focused on the evaluation of the effects of common MS therapies on CTLA-4 modulation or on the study of the CTLA-4 blockade or deficiency in experimental autoimmune encephalomyelitis models. Moreover, other studies in this field aimed to discover if the CTLA-4 gene might be involved in the predisposition to MS, whereas others evaluated the effects of treatment with CTLA4-Ig in MS. Although these results are of great interest, they are often conflicting. Therefore, further studies are needed to reveal the exact mechanisms underlying the action of a crucial immune checkpoint such as CTLA-4 in MS to identify novel immunotherapeutic strategies for MS patients.
Complex Physical Structure of Complete Mitochondrial Genome of Quercus acutissima (Fagaceae): A Significant Energy Plant
Authors: Liu Dan , Guo Haili , Zhu Jingle , Qu Kai , Chen Ying , Guo Yingtian , Ding Ping , Yang Haiping , Xu Ting , Jing Qi , Han Shangjun , Li Wei , Tong Boqiang
Published: July 2022

Quercus acutissima Carruth. is a Chinese important energy plant with high ecological and economic values. While the species chloroplast genome has been reported, its mitochondrial genome (mitogenome) is still unexplored. Here, we assembled and annotated the Q. acutissima mitogenome, and we compared its characteristic differences with several closely related species. The Q. acutissima mitogenome’s main structure is branched with three distinguished contigs (linear molecule 1, circular molecule 2, and circular molecule 3) with 448,982 bp total length and 45.72% GC content. The mitogenome contained 51 genes, including 32 protein-coding, 16 tRNA and 3 rRNA genes. We examined codon usage, repeated sequences, genome recombination, chloroplast to mitochondrion DNA transformation, RNA editing, and synteny in the Q. acutissima mitogenome. Phylogenetic trees based on 29 species mitogenomes clarified the species classification. Our results provided comprehensive information of Q. acutissima mitogenome, and they are expected to provide valuable information for Fagaceae evolutionary biology and to promote the species germplasm utilization.
Source-To-Sink Transport of Sugar and Its Role in Male Reproductive Development
Authors: Li Jingbin , Kim Yu-Jin , Zhang Dabing
Published: July 2022

Sucrose is produced in leaf mesophyll cells via photosynthesis and exported to non-photosynthetic sink tissues through the phloem. The molecular basis of source-to-sink long-distance transport in cereal crop plants is of importance due to its direct influence on grain yield—pollen grains, essential for male fertility, are filled with sugary starch, and rely on long-distance sugar transport from source leaves. Here, we overview sugar partitioning via phloem transport in rice, especially where relevant for male reproductive development. Phloem loading and unloading in source leaves and sink tissues uses a combination of the symplastic, apoplastic, and/or polymer trapping pathways. The symplastic and polymer trapping pathways are passive processes, correlated with source activity and sugar gradients. In contrast, apoplastic phloem loading/unloading involves active processes and several proteins, including SUcrose Transporters (SUTs), Sugars Will Eventually be Exported Transporters (SWEETs), Invertases (INVs), and MonoSaccharide Transporters (MSTs). Numerous transcription factors combine to create a complex network, such as DNA binding with One Finger 11 (DOF11), Carbon Starved Anther (CSA), and CSA2, which regulates sugar metabolism in normal male reproductive development and in response to changes in environmental signals, such as photoperiod.
Impact of COVID-19 on the Changing Patterns of Respiratory Syncytial Virus Infections
Authors: Garg Ishan , Shekhar Rahul , Sheikh Abu Baker , Pal Suman
Published: July 2022

Seasonal epidemics of respiratory syncytial virus (RSV) is one of the leading causes of hospitalization and mortality among children. Preventive measures implemented to reduce the spread of SARS-CoV-2, including facemasks, stay-at-home orders, closure of schools and local-national borders, and hand hygiene, may have also prevented the transmission of RSV and influenza. However, with the easing of COVID-19 imposed restrictions, many regions are noticing a delayed RSV outbreak. Some of these regions have also noted an increase in severity of these delayed RSV outbreaks partly due to a lack of protective immunity in the community following a lack of exposure from the previous season. Lessons learned from the COVID-19 pandemic can be implemented for controlling RSV outbreaks, including: (1) measures to reduce the spread, (2) effective vaccine development, and (3) genomic surveillance tools and computational modeling to predict the timing and severity of RSV outbreaks. These measures can help reduce the severity and prepare the health care system to deal with future RSV outbreaks by appropriate and timely allocation of health care resources.
Rare Earth Elements in Boletus edulis (King Bolete) Mushrooms from Lowland and Montane Areas in Poland
Authors: Falandysz Jerzy , Nnorom Innocent Chidi , Mędyk Małgorzata
Published: July 2022

Mining/exploitation and commercial applications of the rare-earth elements (REEs: La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, and Lu) in the past 3 decades have raised concerns about their emissions to the environment, possible accumulation in food webs, and occupational/environmental health effects. The occurrence and distribution of REEs Y and Sc in the fruitbodies of Boletus edulis collected from geographically diverse regions in Poland were studied in 14 composite samples that were derived from 261 whole fruiting bodies. Individual REE median concentrations ranged from 0.4–95 µg kg−1 dry weight (dw). The summed REE concentrations varied widely, with a median value of 310 µg kg−1 dw and a range of 87 to 758 µg kg−1. The Sc and Y median concentrations (dw) were 35 and 42 µg kg−1, respectively. Ce, La, and Nd, with median values of 95, 51, and 32 µg kg−1, respectively, showed the highest occurrence. B. edulis collected from a forested area formerly used as a military shooting range—possibly a historically contaminated site—had an elevated summed REE content of 1796 µg kg−1. REE concentrations were generally low in Polish King Bolete. Dietary intake from a mushroom meal was negligible, posing no health risk to consumers.
Effect of Cd on Pyrolysis Velocity and Deoxygenation Characteristics of Rice Straw: Analogized with Cd-Impregnated Representative Biomass Components
Authors: Xu Zhi , Guo Zhaohui , Xie Huimin , Hu Yulian
Published: July 2022

The pyrolysis characteristics of cadmium (Cd)-impregnated cellulose, hemicellulose, and lignin were studied to elucidate the pyrolysis velocity and deoxygenation characteristics of Cd-contaminated rice straw. The results show that Cd significantly affects the pyrolysis characteristics of a single biomass component. With a heating rate of 5 °C·min−1 and a Cd loading of 5%, the initial pyrolysis temperature of cellulose and hemicellulose decreases while that of lignin increases. The maximum pyrolysis velocity of cellulose, hemicellulose, and lignin is decreased by 36.6%, 12.4%, and 15.2%, respectively. Cd increases the pyrolysis activation energy of the three components and inhibits their deoxygenation. For the pyrolysis of Cd-contaminated rice straw, both the initial depolymerization temperature and the pyrolysis velocity of hemicellulose is reduced, while the pyrolysis velocity of cellulose is accordingly increased. When Cd loading amplifies to 0.1%, 1%, and 5%, the maximum pyrolysis velocity of hemicellulose is decreased by 7.2%, 10.5%, and 21.3%, while that of cellulose is increased by 8.4%, 62.1%, and 97.3%, respectively. Cd reduces the release of volatiles and gas from rice straw, such as CO2, CO, and oxygen-containing organics, which retains more oxygen and components in the solid fraction. This research suggested that Cd retards the pyrolysis velocity and deoxygenation of rice straw, being therefore beneficial to obtaining more biochar.
Determinants of Cognitive Performance in Children and Adolescents: A Populational Longitudinal Study
Authors: Lima Rodrigo Antunes , Soares Fernanda Cunha , van Poppel Mireille , Savinainen Saija , Mäntyselkä Aino , Haapala Eero A. , Lakka Timo
Published: July 2022

We evaluated the determinants of cognitive performance in children and adolescents. This is a longitudinal study, secondary analysis of the Physical Activity and Nutrition in Children (PANIC) study. We assessed 502 children (51.6% girls) at middle childhood (range: 6.6 to 9.0 years), at late childhood, 437 children (51.0% girls, range: 8.8 to 11.2 years), and in 277 adolescents (54.5% girls, range: 15.0 to 17.4 years). Raven’s progressive matrices tests estimated the participants’ cognitive performance (outcome variable) at all time points. In total, we evaluated 29 factors from various dimensions (prenatal, neonatal, child fitness, lifestyle and anthropometrics). None of the neonatal and anthropometric parameters were associated with cognitive performance. Preeclampsia (prenatal) and listening to music, writing, arts and craft and watching TV (lifestyle) were negatively associated with cognitive performance. Shuttle run and box and block tests (fitness), and playing music, reading and time at the computer (lifestyle) were positive determinants of cognitive performance in children and adolescents. Fitness and lifestyle factors during childhood and adolescence diminished the importance of prenatal factors on cognitive performance and lifestyle factors were especially relevant in regard to cognitive performance. Reading was positively associated with cognitive performance, regardless of age and time dedicated, and should be promoted.
The Impact of 51 Risk Factors on Life Expectancy in Canada: Findings from a New Risk Prediction Model Based on Data from the Global Burden of Disease Study
Authors: Kopec Jacek A. , Sayre Eric C. , Shams Benajir , Li Linda C. , Xie Hui , Feehan Lynne M. , Esdaile John M.
Published: July 2022

The aims of this study were (1) to develop a comprehensive risk-of-death and life expectancy (LE) model and (2) to provide data on the effects of multiple risk factors on LE. We used data for Canada from the Global Burden of Disease (GBD) Study. To create period life tables for males and females, we obtained age/sex-specific deaths rates for 270 diseases, population distributions for 51 risk factors, and relative risk functions for all disease-exposure pairs. We computed LE gains from eliminating each factor, LE values for different levels of exposure to each factor, and LE gains from simultaneous reductions in multiple risk factors at various ages. If all risk factors were eliminated, LE in Canada would increase by 6.26 years for males and 5.05 for females. The greatest benefit would come from eliminating smoking in males (2.45 years) and high blood pressure in females (1.42 years). For most risk factors, their dose-response relationships with LE were non-linear and depended on the presence of other factors. In individuals with high levels of risk, eliminating or reducing exposure to multiple factors could improve LE by several years, even at a relatively advanced age.
Sleep Pattern Is Related to Mental Health among Chinese Collegiate Student Athletes
Authors: Wei Wei , Liu Weimin
Published: July 2022

Regarding the association between sleep and napping duration and mental health in young and older adults, some studies indicated a positive association, while others indicated a negative, or no, association between them. Moreover, collegiate student athletes have different mental health stressors, such as training pressure, improving sports performance, and relationships with coaches. Therefore, sleep is important for athletes. Whether sleep duration is related to their mental health is unclear. Thus, this study aimed to examine the association between nighttime sleep duration, daytime napping duration, and mental health among collegiate student athletees. This cross-sectional study included 700 college athletes. Sleep and daytime napping durations were assessed using a self-reported questionnaire. The Zung Self-rating Depression Scale and Generalized Anxiety Disorder-7 assessed mental health. A multivariate logistic regression analysis was conducted to examine the adjusted association between sleep duration and mental health. In this study, the odds ratios for depression and anxiety symptoms were significantly higher for short sleep duration (<7 h). Additionally, a significant positive association was found between daytime napping duration and the prevalence of depression. This study indicates that short nighttime sleep and long daytime napping duration may be risk factors for collegiate student athletes’ mental health, having important implications for educators and coaches.
How the 5G Enabled the COVID-19 Pandemic Prevention and Control: Materiality, Affordance, and (De-)Spatialization
Authors: Li Gaoyong , Zhang Xin , Zhang Ge
Published: July 2022

5G, the most disruptive innovation, had played a significant role in the COVID-19 pandemic prevention and control. However, as a novel technology and context, we have little knowledge about how 5G enabled pandemic prevention and control. This study collected 212 cases and conducted qualitative research to explore how the 5G worked in prevention and control. Based on the concepts of materiality and affordance, we grounded two affordances of spatialization and de-spatialization from the data. Spatialization provides non-contact ways to complete the tasks which are supposed to be completed in contact, and de-spatialization provides remote operations to complete the tasks which are supposed to be completed on-site. Spatialization and de-spatialization enabled the diagnosis and treatment of the infectors to relieve the unbalance of medical staff, cutting the infectious route to contain the viral spread, and logistic supply to support the prevention and control. Our study offers theoretical contributions to digital pandemic prevention and control, and the literature on 5G also offers practical implications.
Important End-of-Life Topics among Latino Patients and Caregivers Coping with Advanced Cancer
Authors: Rosario-Ramos Lianel , Suarez Keishliany , Peña-Vargas Cristina , Toro-Morales Yoamy , Costas-Muñiz Rosario , Castro Eida , Torres Stephanie , Torres-Blasco Normarie
Published: July 2022

There is a known end-of-life related disparity among Latino individuals, and there is a need to develop culturally sensitive interventions to help patients and caregivers cope with advanced cancer. Latino patients and caregivers coping with advanced cancer were asked to list important end-of-life topics to culturally inform a psychosocial intervention adaptation process. A qualitative study was conducted, and semi-structured interviews were performed, audio-recorded, and transcribed. Recordings and transcriptions were reviewed and analyzed using thematic content coding. The semi-structured interview described and demonstrated intervention components and elicited feedback about each. Free listing method was used to assess important topics among Latino advanced cancer patients (n = 14; stage III and IV) and caregivers. Patients and caregivers were given a list of 15 topics and asked which topics they deemed important to integrate into the intervention. Overall, more than half of the participants considered it important to include 13 of the 15 topics related to daily activities (eight subcategories), psychosocial support (three subcategories), discussing diagnosis and support (three subcategories), and financial difficulties (one subcategory). Patient-caregivers reported importance in most end-of-life topics. Future research and intervention development should include topics related to psychosocial support, daily activities, discussing diagnosis and support, and financial difficulties.