bluuebunny/crossref_metadata_2025 huggingface dataset:
Near-infrared spectroscopy (NIRS) in vivo assessment of skeletal muscle oxidative capacity: a comparison of results from short versus long exercise protocols and reproducibility in non-athletic adults
Authors: Tandirerung Fistra J. , Jamieson Alexandra , Hendrick Elizabeth , Hughes Alun D. , Jones Siana
Published: July 2024

Background

Near-infrared spectroscopy (NIRS) provides a non-invasive, cost-effective method for assessing skeletal muscle oxidative capacity when combined with a short exercise protocol and arterial occlusions. However, the impact of different exercise protocols and reproducibility of the method in non-athletic adults have not previously been assessed.

Methods

Young, non-athletic adults (YA) were invited to perform a short duration, fast frequency contraction (SF) exercise protocol and a long duration slow frequency (LS) contraction protocol, combined with NIRS measurements and arterial occlusions to assess skeletal muscle oxidative capacity. YA and older non-athletic adults (OA; >65 years old) were invited to perform the SF exercise protocol twice to assess the reproducibility of this oxidative capacity measurement.

Results

We included 25 participants (14 male (56%), age range: 18–86 years) in the analyses. There was a strong positive correlation and good agreement between time constants derived following the SF and LS exercise protocols (Lin’s concordance correlation coefficient: 0.69, p-value < 0.001 mean bias [LoA]: −3.2 [−31.0, 24.4] seconds. There was a strong positive correlation and good agreement between time constants derived from the SF exercise protocol in the YA & OA group (Lin’s concordance correlation coefficient: 0.63, p-value < 0.001; mean bias [LoA] −6.4 [−34.0, 21.3] seconds).

Conclusion

These data provide evidence to suggest that NIRS is a reliable in vivo method for the assessment of skeletal muscle oxidative capacity irrespective of exercise protocol duration or muscle contraction frequency. NIRS-measured oxidative capacity via the SF exercise protocol was reproducible in non-athletic adults with a wide range in age.
Italian university students’ future time perspective and well-being during the COVID-19 pandemic
Authors: Parrello Santa , Postiglione Jacopo , Sica Luigia Simona , De Rosa Barbara , Parola Anna , Regnoli Giorgio Maria , Fenizia Elisabetta , Sommantico Massimiliano
Published: July 2024

Introduction

During the COVID-19 pandemic, young adults worldwide showed signs of distress as they were affected in their specific developmental tasks, including the construction of personal and professional futures.

Methods

The present study aimed to assess the situational future time perspective of Italian university students during the second pandemic wave, as measured by an ad hoc constructed instrument, to explore its interaction with some dispositional traits relevant in future construction, such as optimism, sense of life, aggression, and dispositional future time perspective, and to test their effect on psychological well-being. The total sample consisted of 389 subjects (18–35 years, M = 23.5, SD = 4.4).

Results and discussion

The results indicated that the pandemic experience, assessed by surveying specific indicators, negatively affected the future time perspective of students, particularly those dispositionally optimistic and convinced that life has meaning. However, awareness of the negative impact that the pandemic brought to the vision of the future seems to have dampened the levels of depression and stress, while anxiety was found to be related only to dispositional traits. The results also suggested the need for educational and economic policies that help young adults develop confidence in the future and in their ability to build it.
Prevalence of sexually transmitted infections and human papillomavirus in cervical samples from incarcerated women in São Paulo, Brazil: a retrospective single-center study
Authors: Zonta Marco A. , Liljander Anne , Roque Karina B. , Schillert Arne , Kai Marco , dos Santo Flávia A. , de Freitas Giulia Pinheiro , Soane Michel , Cavalar Markus , Janaudis Gustavo , Shio Marina Tiemi
Published: July 2024

Introduction

Sexually transmitted infections (STIs) cause considerable morbidity worldwide and, depending on the specific pathogen, may lead to serious complications in the female reproductive tract. Incarcerated women are particularly vulnerable to health problems with a disproportionate high rate of STIs, including infections with human papillomavirus (HPV).

Methods

Here, cervical swab samples collected from 299 women (18 to 64 years) living in one of the women’s prisons of São Paulo, Brazil were submitted for liquid-based cytology to determine the prevalence of precancerous lesions. Furthermore, direct detection of 30 genital HPV genotypes (18 high-risk and 12 low-risk types) and 11 additional STIs (Chlamydia trachomatis, Neisseria gonorrhoeae, Herpes simplex virus 1 and 2, Haemophilus ducreyi, Mycoplasma genitalium and hominis, Treponema pallidum, Trichomonas vaginalis, Ureaplasma parvum and urealyticum) were performed by molecular typing using two PCR-based DNA microarray systems, i.e., EUROArray HPV and EUROArray STI (EUROIMMUN), respectively.

Results

The overall prevalence of cytological abnormalities was 5.8%, including five women with low-grade and five women with high-grade squamous intraepithelial lesions. The overall prevalence of HPV was 62.2, and 87.1% of the HPV-positive women were infected with oncogenic high-risk (HR) HPV types. HPV types 16 (24.1%), 33 and 52 (both 10.4%) were the most frequently detected. The prevalence of the other STIs was 72.8%. Up to four different pathogens were found in the infected women, the most frequent being Ureaplasma parvum (45.3%), Mycoplasma hominis (36.2%) and Trichomonas vaginalis (24.8%).

Conclusion

The high number of HR-HPV infections and other STIs described here highlights the fact that the Brazilian female prison population requires more attention in the country’s health policies. The implementation of screening programs and treatment measures might contribute to a decrease in the incidence of STIs and cervical cancer in this vulnerable population. However, for such measures to be effective, further studies are needed to investigate the best practice to get more women to engage in in-prison prevention programs, e.g., through offering further sexual health education and self-sampling.
What is known about adolescent dysmenorrhoea in (and for) community health settings?
Authors: Dixon Sharon , Hirst Jennifer , Taghinejadi Neda , Duddy Claire , Vincent Katy , Ziebland Sue
Published: July 2024

Introduction

Dysmenorrhoea affects many adolescents with significant impacts on education and well-being. In the UK, most of the adolescents who seek care (and many never do), will do so through general practice (primary care). Knowing how best to care for adolescents reporting menstrual pain is an area where UK general practitioners would like better guidance and resources.

Methods

This mixed-methods narrative synthesis collates community and specialist evidence from 320 papers about adolescent dysmenorrhoea, with a UK general practice community health perspective.

Results

We report a narrative summary of symptoms, cause, consequences and treatments for adolescent dysmenorrhoea. We highlight areas of tension or conflicted evidence relevant to primary care alongside areas of uncertainty and research gaps identified through this synthesis with input from lived experience advisers

Discussion

There is little evidence about primary care management of adolescent dysmenorrhoea or specific resources to support shared-decision making in general practice, although there are evidence-based treatments to offer. Primary care encounters also represent potential opportunities to consider whether the possibility of underlying or associated health conditions contributing to symptoms of dysmenorrhoea, but there is little epidemiological evidence about prevalence from within community health settings to inform this. The areas where there is little or uncertain evidence along the care journey for adolescent dysmenorrhoea, including at the interface between experience and expression of symptoms and potential underlying contributory causes warrant further exploration.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPEROFILES/256458_STRATEGY_20210608.pdf, identifier (CRD42021256458).
Determination of the reference interval for urinary klotho to creatinine ratio of healthy dogs
Authors: Marečáková Nikola , Kačírová Jana , Tóthová Csilla , Maďari Aladár , Maďar Marián , Farbáková Jana , Horňák Slavomír
Published: July 2024

For several years, alpha klotho has been considered as a candidate biomarker in chronic kidney disease (CKD), progression of CKD and CKD mineral bone disorders (CKD-MBD). The evidence on the relationship between klotho and kidney function is controversial in some areas. The aim of the study was to identify the influence of age, sex and breed on urinary alpha klotho, values in the early stages of CKD within the studied population and determine a reference interval in a group of healthy dogs. Significantly higher values were measured in older dogs over 6 years old (p = 0.026, p = 0.0007) and in the breed German Shepherd than Belgian Shepherd (p = 0.0401). On the basis of sex and in small breed dogs, no significant differences were noted. In dogs with CKD stage 2, alpha klotho values were significantly lower (p = 0.0135) than in healthy dogs. Within the studied population, a reference interval for urinary klotho to creatinine ratio (UrKl/Cr) was determined in the range of 3.94–23.55 pg/gCr. Since our findings show that alpha klotho is associated with older age, we assume that this may have influenced the results in the group of dogs with CKD stage 1 due to the presence of predominantly old dogs in this group. Future studies would be needed to consider age as a factor affecting urinary alpha klotho in dogs with CKD.
High toxinogenic potential of Staphylococcus aureus from wild ungulates in Brandenburg, Germany with a low level of antibiotic resistance
Authors: Lienen Tobias , Mateus-Vargas Rafael Hernán , Steinhoff-Wagner Julia , Richter Martin H. , Maurischat Sven
Published: July 2024

Introduction

Data regarding the occurrence and virulence of Staphylococcus (S.) aureus in wild living animals is rare. However, S. aureus may carry a multitude of virulence factors and express resistance to several antimicrobial substances. Handling game meat may thus lead to serious infections or food poisoning. The aim of this study was to provide insights into the occurrence and characteristics of S. aureus in wild ungulates from Brandenburg, Germany.

Methods

Nasal swabs of externally healthy-looking wild boars, roe, fallow and red deer were collected in hunts during season 2021/2022 and analyzed for S. aureus by selective enrichment. Species were determined using matrix assisted laser desorption ionization mass spectrometry and tested for phenotypic antimicrobial resistance. Whole-genome sequencing was conducted for genotyping, determination of virulence associated genes and analysis of phylogenetic relationships.

Results

S. aureus were recovered from approximately 8% of nasal swabs. However, the strains were only obtained from the sampled wild ruminants. S. aureus isolates were associated with sequence types (ST) 1, ST30, ST133, ST425, ST582 and ST6238. Isolates of ST1 clustered closely together in the phylogenetic analysis. Genes encoding staphylococcal enterotoxin (SE) or SE-like (SEl) were found in 14/17 isolates. In particular, a seh gene was present in 12/17 isolates. Moreover, two isolates harbored a multiplicity of genes encoding SE or SEl. In addition, the toxic shock syndrome toxin encoding tst gene was detected in one isolate. This isolate was resistant to penicillin and cefoxitin and accordingly harbored the blaZ gene.

Discussion

Wild ungulates intended for human consumption may carry potentially virulent S. aureus. In one case, the close phylogenetic relationship of S. aureus isolates indicates a possible intraspecific spread within a common territory. However, for others, the origin or the spread pattern can only be inferred. Handling of animals or their carcasses might contribute to staphylococcal infections in humans. Moreover, food poisoning due to SE producing strains may occur, if recommended hygiene practices are not applied during processing of game meat.
The role of acetylation in obesity-induced cardiac metabolic alterations
Authors: Ketema Ezra B. , Lopaschuk Gary D.
Published: July 2024

Obesity is a growing public health problem, with its prevalence rate having tripled in the last five decades. It has been shown that obesity is associated with alterations in cardiac energy metabolism, which in turn plays a significant role in heart failure development. During obesity, the heart becomes highly dependent on fatty acid oxidation as its primary source of energy (ATP), while the contribution from glucose oxidation significantly decreases. This metabolic inflexibility is associated with reduced cardiac efficiency and contractile dysfunction. Although it is well recognized that alterations in cardiac energy metabolism during obesity are associated with the risk of heart failure development, the molecular mechanisms controlling these metabolic changes are not fully understood. Recently, posttranslational protein modifications of metabolic enzymes have been shown to play a crucial role in cardiac energy metabolic changes seen in obesity. Understanding these novel mechanisms is important in developing new therapeutic options to treat or prevent cardiac metabolic alteration and dysfunction in obese individuals. This review discusses posttranslational acetylation changes during obesity and their roles in mediating cardiac energy metabolic perturbations during obesity as well as its therapeutic potentials.
DNA Methylation Biomarkers in Stool Samples: Enhancing Colorectal Cancer Screening Strategies
Authors: Porcaro Floriana , Voccola Serena , Cardinale Gaetano , Porcaro Piercarmine , Vito Pasquale
Published: July 2024

Colorectal cancer (CRC) is a significant global health challenge, ranking among the leading causes of cancer-related mortality worldwide. Despite efforts in prevention and early detection, CRC incidence and mortality rates are expected to rise substantially. Traditional screening methods like gFOBT, FIT, flexible sigmoidoscopy, colonoscopy, CTC, and colon capsule have limitations, including false positives/negatives, limited scope, or invasiveness. Recent developments in CRC screening involve DNA methylation biomarkers, showing promise in detecting early-stage CRC and precancerous lesions. Stool-based DNA testing is emerging as a noninvasive and convenient method for detecting CRC-associated DNA methylation alterations, offering potential for earlier detection compared to traditional methods. Several commercial stool-based DNA methylation tests targeting different genes associated with CRC have demonstrated varying sensitivity and specificity, some surpassing traditional screening methods. Challenges remain in optimizing their performance and accessibility. This review discusses how DNA methylation biomarkers could enhance CRC screening, and stool-based DNA methylation tests could revolutionize CRC screening practices, comparing them to the gold standard.
Virtuális gyártás alkalmazása ipari gyártóegységek korszerűsítésére
Authors: Palánkai Emese , Sarvajcz Kornél
Published: July 2024

Kutatómunkánk egy hosszú ideje változatlan elveken üzemelő, hagyományos rendszerekkel dolgozó vállalat egyik gyártósorának megismerésére, elemzésére, majd modernizálására terjed ki. A publikáció a cég gyártási folyamatainak korszerűsítésére alkalmas módszerek közül a virtuális gyártás eszközét emeli ki. Átfogó ismereteket ad annak tulajdonságairól, előnyeiről, valamint a folyamatok fejlődésére gyakorolt pozitív hatásairól. A tudományos cikk a kiválasztott valós termelési folyamat áttekintése után a virtuális gyártás megvalósításához létrehozott digitális ikertestvér felépítésének lépéseit mutatja be. Részletesen kifejti a modell megalkotásához felhasznált Tecnomatix Plant Simulation modellalkotó környezet különböző funkcióit és az abban elért eredményeket. A tanulmány a szimuláció kiértékelését követően vizsgálja a valós folyamat jelenlegi állapotát, illetve teljes eszközhatékonyságát, majd a levont következtetések alapján előremutat a gyártósor fejlesztésére vonatkozóan, valamint bemutatja a kutatás továbbhaladásának lehetőségeit.
Internationalization of Small and Medium-Sized Enterprises: Best Practices and the Emerging Concept of Foreign Champion, an Empirical Investigation
Authors: Calheiros-Lobo Nuno , Palma-Moreira Ana , Au-Yong-Oliveira Manuel , Ferreira José Vasconcelos
Published: July 2024

This article introduces a new theoretical concept, the “foreign champion”, which attempts to solve the principal–agent problem and improve academic understanding of foreign market entry decisions by analyzing what experts in the field consider to be best practices. Based on empirical research into the determinants of SME internationalization success, semi-structured interviews (n = 12, t = 5 h 10 min.) were conducted with industry experts, including one academic, government officials, chamber and association leaders, a venture capitalist, and several CEOs, and key testimonies were studied using qualitative analysis software. The results shed light on effective internationalization strategies and common market entry mistakes. Despite the perception of saturation, the prominence of the experts, and the large sample, there may be limitations, such as not covering several regions. The originality of this study lies in its conceptual framework, the insights gained from the select interviews, and a new concept of foreign market entry.
Research on the Design of Aviation and Aerospace Hatch Door Mechanisms and Their Future Bionic Prospects
Authors: Cui Zhiwu , Wu Haochang , Zhou Ming , Cui Zhihe , Huang Hao , Liu Ziyu
Published: July 2024

The design of the space hatch door mechanisms is crucial in the aerospace field, impacting not only durability and reliability but also the life safety of astronauts during space missions. This review extensively researches vehicle doors and hatches in civil and military systems across various environments, including land, sea, deep sea, aviation, aerospace, and extreme conditions. Specially, it focuses on the structural design of hatches and related mechanisms in civil aviation and military aerospace environments, such as opening and closing mechanisms, release mechanisms, locking mechanisms, sealing mechanisms, and the ergonomic design of door structures. The review highlights the integration of bionic design principles with hatch mechanisms to explore future solutions. By systematically examining these aeras, this review addresses the lack of comprehensive studies in previous reviews, which often overlook the interconnectivity and applicability of hatch mechanisms across different fields. The absence of such holistic reviews has led to fragmented knowledge and missed opportunities for cross-industry innovation. This review aims to fill these gaps by providing a wide range of design solutions and offering insights that can enhance the development of more reliable, efficient, and safe hatch mechanisms in aerospace and other high-stakes environments.
Application of Protein Hydrolysate Improved the Productivity of Soybean under Greenhouse Cultivation
Authors: Engel Daniele Caroline Hörz , Feltrim Daniela , Rodrigues Mayara , Baptistella João Leonardo Corte , Mazzafera Paulo
Published: July 2024

Protein hydrolysates are plant biostimulants containing amino acids, oligopeptides, and peptides in their composition. When supplied to plants, protein hydrolysates (HPs) have been identified to improve nitrogen metabolism, enhance the activity of antioxidant enzymes, boost plant defense response to stresses, and positively impact the quantity and quality of products. Soybean is a crucial global commodity, with nitrogen being the primary nutrient for crop development as it directly affects productivity. This study aimed to evaluate the effect of an HP-based biostimulant on the N metabolism in nodulated soybean plants and their productivity. A greenhouse experiment was conducted to test two modes of application of the 0.20% HP-based biostimulant. Soybean plants, growing in pots, were treated with 0.20% HP either via seed treatment or foliar application (at growth stages V3 and V5). Activities of enzymes and compounds related to N metabolism, gene expression, and productivity components were analyzed. It was observed that the mode of application did not significantly influence the results. The application of HPs increased the concentration of nitrate, amino acids, and ureides in soybean leaves. It also positively altered the expression of genes such as nitrate reductase, urease, and asparagine. Additionally, it enhanced productivity, resulting in plants with a greater number and weight of pods and grains. Therefore, it is possible to consider HPs as a stimulator for increasing soybean productivity, even under non-stressing conditions.
Alterations in Soil Bacterial Community and Its Assembly Process within Paddy Field Induced by Integrated Rice–Giant River Prawn (Macrobrachium rosenbergii) Farming
Authors: Zhang Yiyun , Hou Yiran , Jia Rui , Li Bing , Zhu Jian , Ge Xianping
Published: July 2024

Integrated rice–aquatic animal farming has become a vital strategy for enhancing food security. To assess the effects of integrated rice–giant river prawn (Macrobrachium rosenbergii) farming (IRPF) on agricultural ecosystems, we used 16S rRNA gene sequencing to analyze soil bacterial communities in comparison with traditional rice monoculture (RM). Our study revealed that the IRPF did not significantly affect the diversity of the soil bacterial community. However, during the initial culture stage, IRPF markedly increased the relative abundance of the phylum candidate division NC10 and the genus Candidatus Methylomirabilis, enhancing nitrogen-cycling-related functions within the bacterial community. Additionally, IRPF reduced the complexity and stability of these communities in the early to middle culture stages. While stochastic processes usually dominate the assembly of these communities, IRPF restricted bacterial migration and reduced the influence of these stochastic processes. Furthermore, IRPF had a significant impact on environmental factors within paddy soils, strongly correlating with shifts in bacterial communities, particularly through variations in soil nitrite concentration. In conclusion, the influence of IRPF on the bacterial community in paddy soils was primarily observed during the early and middle culture stages, and the impacts of IRPF on the soil bacterial community were primarily driven by environmental changes, especially soil nitrite concentration. These findings provide theoretical insights and a reference for understanding the microbiological impacts of different integrated rice–fish farming systems on agricultural ecosystems.
Improving Total Mixed Ration Silage: Effects of Lactic Acid Bacteria Inoculants and Antimicrobial Additives on Fermentation Quality and Aerobic Stability
Authors: Li Xinbao , Cheng Yuanzhen , Yang Feifei , Hu Junfeng , Ma Rui , Liu Haopeng , Shao Tao
Published: July 2024

This work aimed to assess microbial inoculants (Lactiplantibacillus plantarum and Lentilactobacillus buchneri), chemical additives (natamycin and hexanoic acid), and their combination on fermentation characteristics and aerobic stability in total mixed ration (TMR) silage. The TMR consisted of 30% water bamboo shell (WBS), 10% alfalfa, 20% rice straw, and 40% concentrate. There were six treatments as follows: (1) deionized water (control, CON). (2) lactic acid bacteria (Lactiplantibacillus plantarum + Lentilactobacillus buchneri; LPB, 1 × 106 cfu/g FW). (3) natamycin (NT, 0.02 g/kg FW). (4) hexanoic acid (HA, 0.02 g/kg FW). (5) lactic acid bacteria + natamycin (SLNT, 0.02 g/kg FW). (6) lactic acid bacteria + hexanoic acid (SLHA, 0.02 g/kg FW). After fermentation, laboratory silos (10 L) were opened to assess fermentation quality, followed by a 6-day aerobic stability test. The results showed that all silages were well fermented with high lactic acid (LA) content, low ammonia nitrogen (NH3-N), and negligible butyric acid (BA) levels. Among all silages, SLNT silage exhibited the greatest LA, acetic acid (AA) levels, LAB counts, and the lowest pH and NH3-N. For aerobic stability, all additives significantly (p < 0.05) enhanced aerobic stability, delayed (p < 0.05) the decrease in LA and water-soluble carbohydrates (WSC) and the increase in pH, and significantly (p < 0.05) minimized yeast proliferation. The SLNT silage showed the best aerobic stability, with SLHA, NT, HA, and LPB following. In conclusion, SLNT is recommended as the optimal additive in improving the fermentation quality and aerobic stability of TMR silage, with SLHA, NT, HA, and LPB following.
Integrated Analysis of the lncRNA-miRNA-mRNA Expression Profiles in Response to Meloidogyne incognita in Radish (Raphanus sativus L.)
Authors: Luo Xiaobo , Jin Yueyue , Shen Feng , Zhang Wanping
Published: July 2024

Radish is an important root vegetable that is widely grown in Asia. The root-knot nematode (RKN) Meloidogyne incognita seriously affects the growth and development of radish root and causes poor appearance quality. However, the molecular mechanism of radish response to RKNs remains poorly understood. In this study, a total of 220 lncRNAs, 1144 mRNAs, 20 miRNAs and 153 proteins were differential expressed between the RKN-infected and WT samples. Correlation analysis of all DEPs compared with all DGEs showed that 8 mRNAs-DEPs showed a changed abundance. The results showed that 18 DEmiRNAs have 167 target DEGs in 220 miRNA-target modules and 29 DElncRNAs were predicted as putative targets of 16 DEmiRNAs in 37 miRNA-target modules. In all, 6 DGEs in the ABA pathway and 2 DGEs in the JA pathway were identified under RKN infection, respectively. The four regulatory networks of lncRNA-miRNA-mRNA were constructed in response to RKN infection. qRT-PCR analysis found that the expression pattern of 6 DElncRNAs, 6 DEmRNAs, 6 DEmiRNAs and 6 DEPs were consistent with sequencing results. These results provide a theoretical basis for studying the molecular mechanism of radish in response to M. incognita and breeding resistant varieties to this nematode.
Comparative Transcriptomic Analysis Reveals the Involvement of Auxin Signaling in the Heat Tolerance of Pakchoi under High-Temperature Stress
Authors: Yang Bing , Chen Yaosong , Li Xiaofeng , Gao Lu , Miao Liming , Song Yishan , Zhang Dingyu , Zhu Hongfang
Published: July 2024

Pakchoi is a kind of nonheading Chinese cabbage being widely cultivated not only in China but also all over Asia. High temperature is a major limiting factor influencing the yield and quality of pakchoi, while the mechanism of pakchoi dealing with high-temperature challenges remains largely elusive. In the present study, we conducted a comparative transcriptomic analysis, which was also validated by qPCR, of the heat-tolerant Xinxiaqing (XXQ) variant and Suzhouqing (SZQ) variant, which are heat-sensitive under high-temperature treatment. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses suggest that high-temperature-induced phytohormones signal transduction, especially auxin signal transduction, regulates the heat responses of pakchoi. Our further investigations imply that high-temperature-activated auxin signal plays a positive role in helping pakchoi deal with high-temperature challenge; IAA-pretreated pakchoi plants exhibited greater resistance to the high-temperature treatment, probably due to the induction of antioxidant activity. In addition, our study also identified six heat shock proteins/factors (HSPs/HSFs) whose up-regulation correlates with the elevated heat tolerance of pakchoi. Notably, among these high-temperature-induced heat-responsive factors, HSP20 and HSP26.5 are under the regulation of auxin signal, and this signal cascade contributes to enhancing the thermostability of pakchoi. In the present study, we identified crucial high-temperature-responsive factors and signaling pathways in pakchoi, which help in understanding the mechanism of pakchoi coping with high-temperature challenge.
Assessing the Impact of Tillage Methods on Soil Moisture Content and Crop Yield in Hungary
Authors: Modiba Maimela Maxwell , Ocansey Caleb Melenya , Ibrahim Hanaa Tharwat Mohamed , Birkás Márta , Dekemati Igor , Simon Barbara
Published: July 2024

A decline in rainfall as a source of agricultural water has affected and will continue to affect sustainable crop production globally including in Hungary. Conservation of the greatest water reservoir is important for the sustainable development of agriculture in Hungary. The objective of this study was to evaluate the effects of the different tillage methods on soil moisture content, grain yield, and root weight of wheat (Triticum aestivum) and sunflower (Helianthus annuus) under rainfed conditions. A field study was conducted at the Józsefmajor Experimental and Training Farm (JM) of the Hungarian University of Agriculture and Life Sciences near Hatvan. The experiment consisted of six tillage treatments: disking (D, 16 cm), shallow cultivation (SC, 20 cm), no-till (NT), deep cultivation (DC, 25 cm), loosening (L, 45 cm), and plowing (P, 30 cm). Soil moisture content (SMC) was measured monthly, and grain yield and root weight were measured at the end of the cropping period. Our results showed no significant difference in SMC between conservation and conventional tillage methods in 2018. However, in 2021, greater SMC was significantly conserved under NT compared to P. Regarding the sampling date, a significant increase in moisture with time was observed. A significantly lower SMC was observed on 3 June 2019 between L and D. while on the 9 September 2020, SMC significantly differed between P and all the other treatments (D, SC, NT, DC, and L). Interestingly in 2018, SMC was significantly lower at 10–20 cm depth between L and D. Notably the effect of depth on SMC was observed as moisture significantly increased with increasing depth in all tillage treatments. Root weight was greatest at DC (1.54 t ha−1) in 2018 and under L (3.89 t ha−1) in 2021. Similarly, wheat grain yield was greatest at DC (2.48 t ha−1) in 2018, while sunflower yield in 2021 was greatest at L (3.86 t ha−1). It is comprehensible that conservation tillage methods such as L and NT can increase SMC and grain yield.
Benchmarking Giant Panda Welfare in Tourism: A Co-Design Approach for Animals, Tourists, Managers, and Researchers
Authors: Guo Yulei , Fennell David
Published: July 2024

This study introduces a co-design benchmarking framework to understand tourists’ perceptions of animal welfare, integrating diverse perspectives from tourists, researchers, and animals. By leveraging scientific theories to establish benchmark dimensions, the framework is refined through visitor input, ensuring a robust and adaptable methodological tool for assessing tourists’ perceptions and animal informed consent in wildlife tourism. Using the Chengdu Research Base of Giant Panda Breeding as an example, we analyzed 4839 visitor comments collected from March to August 2023 to benchmark perceptions of giant panda welfare. This approach underscores the importance of effective communication in educational initiatives, aiming to enhance public literacy and knowledge about animal welfare. By addressing the complexity and variability in tourists’ perceptions, the proposed framework contributes to more impactful conservation education efforts. The study demonstrates that a collaborative effort results in a benchmarking framework that is firmly grounded in theoretical foundations yet flexible enough to adapt based on visitors’ insights and animal participation. Ultimately, this comprehensive approach ensures that educational initiatives resonate with tourists’ diverse backgrounds, fostering a deeper understanding and commitment to animal welfare and conservation, which, we argue, should be key components of sustainable tourism.
Follicular Atresia in Buffalo: Cocaine- and Amphetamine-Regulated Transcript (CART) and the Underlying Mechanisms
Authors: Yang Chunyan , Zheng Haiying , Amin Ahmed , Faheem Marwa S. , Duan Anqin , Li Lingyu , Xiao Peng , Li Mengqi , Shang Jianghua
Published: July 2024

Atresia is a process in ovarian follicles that is regulated by hormone-induced apoptosis. During atresia, granulosa cell (GC) apoptosis is a key mechanism orchestrated through diverse signaling pathways. Cocaine- and amphetamine-regulated transcript (CART) signaling within ovarian GCs has been demonstrated to play a key role in the regulation of follicular atresia in cattle, pigs, and sheep. The present work aimed to investigate the potential local regulatory role of CART in GC apoptosis-induced follicular atresia in buffalo, focusing on the modulation of the AKT/GSK3β/β-catenin signaling pathways, which are the intracellular signaling pathways involved in cell viability. Our findings revealed increased expression of CARTPT and BAX and decreased levels of AKT, β-catenin, and CYP19A1 genes in atretic follicles compared to healthy follicles. Subsequently, CART treatment in the presence of FSH inhibited the FSH-induced increase in GC viability by reducing estradiol production and increasing apoptosis. This change was accompanied by an increase in the gene expression levels of both CARTPT and BAX. At the protein level, treatment with CART in the presence of FSH negatively affected the activity of AKT, β-catenin, and LEF1, while the activity of GSK3β was enhanced. In conclusion, our study shows how CART negatively influences buffalo GC viability, underlying the modulation of the AKT/GSK3β/β-catenin pathway and promoting apoptosis—a key factor in follicular atresia.
Testosterone Inhibits Lipid Accumulation in Porcine Preadipocytes by Regulating ELOVL3
Authors: Xie Fuyin , Wang Yubei , Chan Shuheng , Zheng Meili , Xue Mingming , Yang Xiaoyang , Luo Yabiao , Fang Meiying
Published: July 2024

Castration is commonly used to reduce stink during boar production. In porcine adipose tissue, castration reduces androgen levels resulting in metabolic disorders and excessive fat deposition. However, the underlying detailed mechanism remains unclear. In this study, we constructed porcine preadipocyte models with and without androgen by adding testosterone exogenously. The fluorescence intensity of lipid droplet (LD) staining and the fatty acid synthetase (FASN) mRNA levels were lower in the testosterone-treated cells than in the untreated control cells. In contrast, the mRNA levels of adipose triglycerides lipase (ATGL) and androgen receptor (AR) were higher than in the testosterone-treated cells than in the control cells. Subsequently, transcriptomic sequencing of porcine preadipocytes incubated with and without testosterone showed that the mRNA expression levels of very long-chain fatty acid elongase 3 (ELOVL3), a key enzyme involved in fatty acids synthesis and metabolism, were high in control cells. The siRNA-mediated knockdown of ELOVL3 reduced LD accumulation and the mRNA levels of FASN and increased the mRNA levels of ATGL. Next, we conducted dual-luciferase reporter assays using wild-type and mutant ELOVL3 promoter reporters, which showed that the ELOVL3 promoter contained an androgen response element (ARE); furthermore, its transcription was negatively regulated by AR overexpression. In conclusion, our study reveals that testosterone inhibits fat deposition in porcine preadipocytes by suppressing ELOVL3 expression. Moreover, our study provides a theoretical basis for further studies on the mechanisms of fat deposition caused by castration.
Preanalytical Stability of 13 Antibiotics in Biological Samples: A Crucial Factor for Therapeutic Drug Monitoring
Authors: Dalla Zuanna Paolo , Curci Debora , Lucafò Marianna , Addobbati Riccardo , Fabretto Antonella , Stocco Gabriele
Published: July 2024

The stability of antibiotic preanalytical samples is a critical factor in therapeutic drug monitoring (TDM), a practice of undoubted importance for the proper therapeutic use of antibiotics, especially in complex management patients, such as pediatrics. This review aims to analyze the data in the literature regarding the preanalytical stability of some of the antibiotics for which TDM is most frequently requested. The literature regarding the preanalytical stability of amikacin, ampicillin, cefepime, ceftazidime, ciprofloxacin, daptomycin, gentamicin, levofloxacin, linezolid, meropenem, piperacillin, teicoplanin, and vancomycin in plasma, serum, whole blood, and dried blood/plasma spot samples was analyzed. Various storage temperatures (room temperature, 4 °C, −20 °C, and −80 °C) and various storage times (from 1 h up to 12 months) as well as subjecting to multiple freeze–thaw cycles were considered. The collected data showed that the non-beta-lactam antibiotics analyzed were generally stable under the normal storage conditions used in analytical laboratories. Beta-lactam antibiotics have more pronounced instability, particularly meropenem, piperacillin, cefepime, and ceftazidime. For this class of antibiotics, we suggest that storage at room temperature should be limited to a maximum of 4 h, storage at 2–8 °C should be limited to a maximum of 24 h, and storage at −20 °C should be limited to a maximum of 7 days; while, for longer storage, freezing at −80 °C is suggested.
Rapid and Simple Morphological Assay for Determination of Susceptibility/Resistance to Combined Ciprofloxacin and Ampicillin, Independently, in Escherichia coli
Authors: López Isidoro , Otero Fátima , Fernández María del Carmen , Bou Germán , Gosálvez Jaime , Fernández José Luis
Published: July 2024

Current antibiograms cannot discern the particular effect of a specific antibiotic when the bacteria are incubated with a mixture of antibiotics. To prove that this task is achievable, Escherichia coli strains were treated with ciprofloxacin for 45 min, immobilized on a slide and stained with SYBR Gold. In susceptible strains, the nucleoid relative surface started to decrease near the MIC, being progressively condensed as the dose increased. The shrinkage level correlated with the DNA fragmentation degree. Ciprofloxacin-resistant bacilli showed no change. Additionally, E. coli strains were incubated with ampicillin for 45 min and processed similarly. The ampicillin-susceptible strain revealed intercellular DNA fragments that increased with dose, unlike the resistant strain. Co-incubation with both antibiotics revealed that ampicillin did not modify the nucleoid condensation effect of ciprofloxacin, whereas the quinolone partially decreased the background of DNA fragments induced by ampicillin. Sixty clinical isolates, with different combinations of susceptibility-resistance to each antibiotic, were co-incubated with the EUCAST breakpoints of susceptibility of ciprofloxacin and ampicillin. The morphological assay correctly categorized all the strains for each antibiotic in 60 min, demonstrating the feasible independent evaluation of a mixture of quinolone and beta-lactam. The rapid phenotypic assay may shorten the incubation times and necessary microbial mass currently required for evaluation.
Rod-Shaped Mesoporous Zinc-Containing Bioactive Glass Nanoparticles: Structural, Physico-Chemical, Antioxidant, and Immuno-Regulation Properties
Authors: Zhu Xiuan , Wen Wenjie , Yan Jingjing , Wang Yuran , Wang Rumeng , Ma Xiang , Ren Dandan , Zheng Kai , Deng Chao , Zhang Jue
Published: July 2024

Bioactive glass nanoparticles (BGNs) are applied widely in tissue regeneration. Varied micro/nanostructures and components of BGNs have been designed for different applications. In the present study, nanorod-shaped mesoporous zinc-containing bioactive glass nanoparticles (ZnRBGNs) were designed and developed to form the bioactive content of composite materials for hard/soft tissue repair and regeneration. The nanostructure and components of the ZnRBGNs were characterized, as were their cytocompatibility and radical-scavenging activity in the presence/absence of cells and their ability to modulate macrophage polarization. The ZnRBGNs possessed a uniform rod shape (length ≈ 500 nm; width ≈ 150 nm) with a mesoporous structure (diameter ≈ 2.4 nm). The leaching liquid of the nanorods at a concentration below 0.5 mg/mL resulted in no cytotoxicity. More significant improvements in the antioxidant and M1-polarization-inhibiting effects and the promotion of M2 polarization were found when culturing the cells with the ZnRBGNs compared to when culturing them with the RBGNs. The doping of the Zn element in RBGNs may lead to improved antioxidant and anti-inflammatory effects, which may be beneficial in tissue regeneration/repair.
Ten “Cheat Codes” for Measuring Oxidative Stress in Humans
Authors: Cobley James N. , Margaritelis Nikos V. , Chatzinikolaou Panagiotis N. , Nikolaidis Michalis G. , Davison Gareth W.
Published: July 2024

Formidable and often seemingly insurmountable conceptual, technical, and methodological challenges hamper the measurement of oxidative stress in humans. For instance, fraught and flawed methods, such as the thiobarbituric acid reactive substances assay kits for lipid peroxidation, rate-limit progress. To advance translational redox research, we present ten comprehensive “cheat codes” for measuring oxidative stress in humans. The cheat codes include analytical approaches to assess reactive oxygen species, antioxidants, oxidative damage, and redox regulation. They provide essential conceptual, technical, and methodological information inclusive of curated “do” and “don’t” guidelines. Given the biochemical complexity of oxidative stress, we present a research question-grounded decision tree guide for selecting the most appropriate cheat code(s) to implement in a prospective human experiment. Worked examples demonstrate the benefits of the decision tree-based cheat code selection tool. The ten cheat codes define an invaluable resource for measuring oxidative stress in humans.
The Double-Edged Sword of ROS in Muscle Wasting and COPD: Insights from Aging-Related Sarcopenia
Authors: Chan S. M. H. , Selemidis S. , Vlahos R.
Published: July 2024

An elevation in reactive oxygen species (ROS) is widely accepted to be a key mechanism that drives chronic obstructive pulmonary disease (COPD) and its major co-morbidity, skeletal muscle wasting. However, it will be perhaps a surprise to many that an elevation in ROS in skeletal muscle is also a critical process for normal skeletal muscle function and in the adaptations to physical exercise. The key message here is that ROS are not solely detrimental. This duality of ROS suggests that the mere use of a broad-acting antioxidant is destined to fail in alleviating skeletal muscle wasting in COPD because it will also be influencing critical physiological ROS-dependent processes. Here, we take a close look at this duality of ROS in skeletal muscle physiology and pathophysiology pertaining to COPD and will aim to gain critical insights from other skeletal muscle wasting conditions due to aging such as sarcopenia.
Adaptive Frame Sampling and Feature Alignment for Multi-Frame Infrared Small Target Detection
Authors: Yao Chuanhong , Zhao Haitao
Published: July 2024

In recent years, infrared images have attracted widespread attention, due to their extensive application in low-visibility search and rescue, forest fire monitoring, ground target monitoring, and other fields. Infrared small target detection technology plays a vital role in these applications. Although there has been significant research over the years, accurately detecting infrared small targets in complex backgrounds remains a significant challenge. Multi-frame detection methods can significantly improve detection performance in these cases. However, current multi-frame methods face difficulties in balancing the number of input frames and detection speed, and cannot effectively handle the background motion caused by movement of the infrared camera. To address these issues, we propose an adaptive frame sampling method and a detection network aligned at the feature level. Our adaptive frame sampling method uses mutual information to measure motion changes between adjacent frames, construct a motion distribution, and sample frames with uniform motion based on the averaged motion distribution. Our detection network handles background motion by predicting a homography flow matrix that aligns features at the feature level. Extensive evaluation of all components showed that the proposed method can more effectively perform multi-frame infrared small target detection.
Laboratory Evaluation of Wear Particle Emissions and Suspended Dust in Tire–Asphalt Concrete Pavement Friction
Authors: Lee Jongsub , Kwon Ohsun , Hwang Yujoong , Yeon Gyumin
Published: July 2024

This study aims to evaluate the tire–road-wear particles (TRWPs) and suspended dust generated based on the nominal maximum aggregate size (NMAS) of the polymer-modified stone mastic asphalt (SMA) mixtures indoors. The SMA mixtures containing styrene butadiene styrene (SBS) polymer and the NMASs of 19, 13, 10, 8, and 6 mm were used. Dust was generated from the wear of the tires and the pavement inside the indoor chamber by using the laboratory tire–road-wear particle generation and evaluation tester (LTRWP tester) developed by Korea Expressway Corporation (KEC). In this method, a cylindrical asphalt-mixture specimen rotates in the center, and a load is applied using three tires on the sides of the test specimen. During the test, a digital sensor was used to measure the concentration for each particle size. After the test was completed, the dust was collected and weighed. According to the test results, the generated TRWP emissions were reduced by approximately 0.15 g as the NMAS of the SMA mixture decreased by 1 mm. TRWP emissions decreased by 20% when using the 6 mm SMA mixture compared to the 13 mm SMA mixture. For practical application, a predicted equation of TRWP emissions estimation was developed by using the concentration of suspended dust measured by the digital sensor in the LTRWP tester. LTRWP can be used as an indoor test method to evaluate pavement and tire materials to reduce the amount of dust generated from tire and pavement wear.
Evolution of Industrial Robots from the Perspective of the Metaverse: Integration of Virtual and Physical Realities and Human–Robot Collaboration
Authors: You Jing , Wu Zhiyuan , Wei Wei , Li Ning , Yang Yuhua
Published: July 2024

During the transition from Industry 4.0 to Industry 5.0, industrial robotics technology faces the need for intelligent and highly integrated development. Metaverse technology creates immersive and interactive virtual environments, allowing technicians to perform simulations and experiments in the virtual world, and overcoming the limitations of traditional industrial operations. This paper explores the application and evolution of metaverse technology in the field of industrial robotics, focusing on the realization of virtual–real integration and human–machine collaboration. It proposes a design framework for a virtual–real interaction system based on the ROS and WEB technologies, supporting robot connectivity, posture display, coordinate axis conversion, and cross-platform multi-robot loading. This paper emphasizes the study of two key technologies for the system: virtual–real model communication and virtual–real model transformation. A general communication mechanism is designed and implemented based on the ROS, using the ROS topic subscription to achieve connection and real-time data communication between physical robots and virtual models, and utilizing URDF model transformation technology for model invocation and display. Compared with traditional simulation software, i.e., KUKA Sim PRO (version 1.1) and RobotStudio (version 6.08), the system improves model loading by 45.58% and 24.72%, and the drive response by 41.50% and 28.75%. This system not only supports virtual simulation and training but also enables the operation of physical industrial robots, provides persistent data storage, and supports action reproduction and offline data analysis and decision making.