bluuebunny/crossref_metadata_2025 huggingface dataset:
Factors affecting the health-promoting behavior of thyroid cancer survivors: comparison by stage of cancer survivorship
Authors: Park Kyung Ah , Kim Sanghee , Oh Eui Geum , Kim Heejung , Chang Hang-Seok , Kim Soo Hyun
Published: January 2022

Purpose

The purpose of this study was to identify differences in factors affecting health-promoting behaviors according to the survival stage of thyroid cancer survivors.

Methods

This descriptive cross-sectional study analyzed data from 354 thyroid cancer survivors after diagnosis. The survivors were divided into three stages: (1) the acute stage (< 2 years after diagnosis), (2) extended stage (2–5 years after diagnosis), and (3) permanent stage (≥ 5 years after diagnosis). To measure health-promoting behavior, the revised Korean version of the Health Promoting Lifestyle Profile questionnaires was used. The factors affecting the health-promoting behavior included social support, self-efficacy, fear of recurrence, and symptoms. Multiple regression analysis was used to analyze factors affecting the health-promoting behavior according to survival stage.

Result

The factors affecting the health-promoting behavior of thyroid cancer survivors differed by survival stage. In the acute stage, the factors of health-promoting behavior were self-efficacy (t = 4.76, p < .001) and social support (t = 3.54, p < .001). In the extended stage, symptoms (t =  − 3.65, p < .001), social support (t = 2.61, p = .011), fear of recurrence (t = 2.18, p = .032), and receipt of radioiodine treatment (t =  − 2.18, p = .032) were found to be significant variables that affected health-promoting behaviors. In the permanent stage, social support (t = 2.79, p = .007), receipt of radioiodine treatment (t =  − 3.21, p = .002), and age (t =  − 2.77, p = .007) were significant variables that affected health-promoting behaviors.

Conclusion

The experience of thyroid cancer survivors varies as they progress through the survival stages; thus, health-promotion interventions should be tailored to each survival stage.
Stir bar sorptive-dispersive microextraction by a poly(methacrylic acid-co-ethylene glycol dimethacrylate)-based magnetic sorbent for the determination of tricyclic antidepressants and their main active metabolites in human urine
Authors: Vállez-Gomis Víctor , Exojo-Trujillo Sara , Benedé Juan L. , Chisvert Alberto , Salvador Amparo
Published: January 2022

A poly(methacrylic acid-co-ethylene glycol dimethacrylate)-based magnetic sorbent was used for the rapid and sensitive determination of tricyclic antidepressants and their main active metabolites in human urine. This material was characterized by magnetism measurements, zeta potential, scanning electron microscopy, nitrogen adsorption–desorption isotherms, and thermogravimetric analysis. The proposed analytical method is based on stir bar sorptive-dispersive microextraction (SBSDME) followed by liquid chromatography–tandem mass spectrometry. The main parameters involved in the extraction step were optimized by using the response surface methodology as a multivariate optimization method, whereas a univariate approach was employed to study the desorption parameters. Under the optimized conditions, the proposed method was properly validated showing good linearity (at least up to 50 ng mL−1) and enrichment factors (13–22), limits of detection and quantification in the low ng L−1 range (1.4–7.0 ng L−1), and good intra- and inter-day repeatability (relative standard deviations below 15%). Matrix effects were observed for the direct analysis of urine samples, but they were negligible when a 1:1 v/v dilution with deionized water was performed. Finally, the method was successfully applied to human urine samples from three volunteers, one of them consuming a prescribed drug for depression that tested positive for clomipramine and its main active metabolite. Quantitative relative recoveries (80–113%) were obtained by external calibration. The present work expands the applicability of the SBSDME to new analytes and new types of magnetic sorbents.

Graphical abstract
Immediately provisionalized tapered conical connection implants for single-tooth restorations in the maxillary esthetic zone: a 5-year prospective single-cohort multicenter analysis
Authors: Baer Russell A. , Nölken Robert , Colic Snjezana , Heydecke Guido , Mirzakhanian Christine , Behneke Alexandra , Behneke Nikolaus , Gottesman Edward , Ottria Liliana , Pozzi Alessandro , Fügl Alexander , Zechner Werner
Published: January 2022

Objectives

This open, single-cohort, multicenter, prospective study investigated the efficacy of immediately provisionalized tapered conical connection implant for single-tooth restorations in the anterior and premolar regions of the maxilla after 5 years of function.

Materials and methods

All implants were placed in healed sites and immediately provisionalized. MBLs, soft-tissue parameters, and oral-health impact profile (OHIP) were evaluated at implant insertion, 6, 12, 24, 36, and 60 months. Paired Wilcoxon signed-rank tests and Kaplan–Meier survival analysis was used for statistical and implant survival/success analyses, respectively.

Results

Seventy-seven patients (81 implants) completed the 5-year follow-up. The 5-year cumulative survival and success rates were 97.8%, and the mean MBL change from implant insertion to 5 years was − 0.80 ± 1.13 mm. Optimal papilla index scores were observed at 90.1% of sites at 5 years compared with 32.8% of sites at insertion. Pink esthetic score, modified bleeding and plaque indices, and OHIP showed statistically significant improvement at the 5-year follow-up.

Conclusions

Immediately provisionalized tapered conical connection implants promote marginal bone stability and excellent esthetic outcomes after 5 years of function.

Clinical relevance

This treatment is a viable option for patients requiring immediately provisionalized single-tooth restorations in the esthetic zone and shows favorable long-term clinical outcomes, including marginal bone stability and excellent esthetics.
Computing top-k temporal closeness in temporal networks
Authors: Oettershagen Lutz , Mutzel Petra
Published: January 2022

The closeness centrality of a vertex in a classical static graph is the reciprocal of the sum of the distances to all other vertices. However, networks are often dynamic and change over time. Temporal distances take these dynamics into account. In this work, we consider the harmonic temporal closeness with respect to the shortest duration distance. We introduce an efficient algorithm for computing the exact top-ktemporal closeness values and the corresponding vertices. The algorithm can be generalized to the task of computing all closeness values. Furthermore, we derive heuristic modifications that perform well on real-world data sets and drastically reduce the running times. For the case that edge traversal takes an equal amount of time for all edges, we lift two approximation algorithms to the temporal domain. The algorithms approximate the transitive closure of a temporal graph (which is an essential ingredient for the top-kalgorithm) and the temporal closeness for all vertices, respectively, with high probability. We experimentally evaluate all our new approaches on real-world data sets and show that they lead to drastically reduced running times while keeping high quality in many cases. Moreover, we demonstrate that the top-ktemporal and static closeness vertex sets differ quite largely in the considered temporal networks.
Can competition improve hospital quality of care? A difference-in-differences approach to evaluate the effect of increasing quality transparency on hospital quality
Authors: Strumann Christoph , Geissler Alexander , Busse Reinhard , Pross Christoph
Published: January 2022

Public reporting on the quality of care is intended to guide patients to the provider with the highest quality and to stimulate a fair competition on quality. We apply a difference-in-differences design to test whether hospital quality has improved more in markets that are more competitive after the first public release of performance data in Germany in 2008. Panel data from 947 hospitals from 2006 to 2010 are used. Due to the high complexity of the treatment of stroke patients, we approximate general hospital quality by the 30-day risk-adjusted mortality rate for stroke treatment. Market structure is measured (comparatively) by the Herfindahl–Hirschman index (HHI) and by the number of hospitals in the relevant market. Predicted market shares based on exogenous variables only are used to compute the HHI to allow a causal interpretation of the reform effect. A homogenous positive effect of competition on quality of care is found. This effect is mainly driven by the response of non-profit hospitals that have a narrow range of services and private for-profit hospitals with a medium range of services. The results highlight the relevance of outcome transparency to enhance hospital quality competition.
The role of central autonomic nervous system dysfunction in Takotsubo syndrome: a systematic review
Authors: Brazdil Vojtech , Kala Petr , Hudec Martin , Poloczek Martin , Kanovsky Jan , Stipal Roman , Jerabek Petr , Bocek Otakar , Pail Martin , Brazdil Milan
Published: January 2022

Introduction

Takotsubo syndrome (TTS), also known as stress cardiomyopathy or “broken heart” syndrome, is a mysterious condition that often mimics an acute myocardial infarction. Both are characterized by left ventricular systolic dysfunction. However, this dysfunction is reversible in the majority of TTS patients.

Purpose

Recent studies surprisingly demonstrated that TTS, initially perceived as a benign condition, has a long-term prognosis akin to myocardial infarction. Therefore, the health consequences and societal impact of TTS are not trivial. The pathophysiological mechanisms of TTS are not yet completely understood. In the last decade, attention has been increasingly focused on the putative role of the central nervous system in the pathogenesis of TTS.

Conclusion

In this review, we aim to summarize the state of the art in the field of the brain–heart axis, regional structural and functional brain abnormalities, and connectivity aberrancies in TTS.
Towards an integrated neonatal brain and cardiac examination capability at 7 T: electromagnetic field simulations and early phantom experiments using an 8-channel dipole array
Authors: Clément Jérémie , Tomi-Tricot Raphaël , Malik Shaihan J. , Webb Andrew , Hajnal Joseph V. , Ipek Özlem
Published: January 2022

Objective

Neonatal brain and cardiac imaging would benefit from the increased signal-to-noise ratio levels at 7 T compared to lower field. Optimal performance might be achieved using purpose designed RF coil arrays. In this study, we introduce an 8-channel dipole array and investigate, using simulations, its RF performances for neonatal applications at 7 T.

Methods

The 8-channel dipole array was designed and evaluated for neonatal brain/cardiac configurations in terms of SAR efficiency (ratio between transmit-field and maximum specific-absorption-rate level) using adjusted dielectric properties for neonate. A birdcage coil operating in circularly polarized mode was simulated for comparison. Validation of the simulation model was performed on phantom for the coil array.

Results

The 8-channel dipole array demonstrated up to 46% higher SAR efficiency levels compared to the birdcage coil in neonatal configurations, as the specific-absorption-rate levels were alleviated. An averaged normalized root-mean-square-error of 6.7% was found between measured and simulated transmit field maps on phantom.

Conclusion

The 8-channel dipole array design integrated for neonatal brain and cardiac MR was successfully demonstrated, in simulation with coverage of the baby and increased SAR efficiency levels compared to the birdcage. We conclude that the 8Tx-dipole array promises safe operating procedures for MR imaging of neonatal brain and heart at 7 T.
Correlation between TXNRD1/HO-1 expression and response to neoadjuvant chemoradiation therapy in patients with esophageal squamous cell carcinoma
Authors: Akaishi Ryujiro , Fujishima Fumiyoshi , Ishida Hirotaka , Tsunokake Junichi , Yamauchi Takuro , Gokon Yusuke , Ueki Shunsuke , Fukutomi Toshiaki , Okamoto Hiroshi , Takaya Kai , Sato Chiaki , Taniyama Yusuke , Nakamura Tomohiro , Nakaya Naoki , Kamei Takashi , Sasano Hironobu
Published: January 2022

Background

Thioredoxin reductase 1 (TXNRD1) and heme oxygenase-1 (HO-1) are both involved in the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway and play key roles in antioxidant responses. In patients with esophageal squamous cell carcinoma (ESCC), the correlation between the expression of these two proteins and the therapeutic response to neoadjuvant chemoradiation therapy (NACRT), as well as the difference in their expression after chemoradiotherapy, remains unknown.

Methods

Proteins involved in the Nrf2 pathway were immunolocalized in carcinoma cells in ESCC patients on NACRT with 5-fluorouracil and cisplatin, followed by esophagectomy. The 8-hydroxydeoxyguanosine (8-OHdG) levels were used to quantify reactive oxygen species. The changes in immunoreactivity before and after NACRT (Δ) were assessed.

Results

Tumor reduction following NACRT was significantly attenuated in pre-therapeutic biopsy specimens associated with high HO-1 status. TXNRD1Δ, HO-1Δ, and 8-OHdGΔ were significantly different in the ineffective and effective groups. The overall survival was significantly lower in high Nrf2 and TXNRD1 groups. In addition, high TXNRD1 expression was an independent prognostic factor in the multivariate analysis of overall survival.

Conclusions

The study findings indicate that HO-1 status in pre-therapeutic biopsy specimens could predict response to NACRT, and TXNRD1 status could predict overall survival of ESCC patients.
PHD2 deletion in endothelial or arterial smooth muscle cells reveals vascular cell type-specific responses in pulmonary hypertension and fibrosis
Authors: Elamaa Harri , Kaakinen Mika , Nätynki Marjut , Szabo Zoltan , Ronkainen Veli-Pekka , Äijälä Ville , Mäki Joni M. , Kerkelä Risto , Myllyharju Johanna , Eklund Lauri
Published: January 2022

Hypoxia plays an important regulatory role in the vasculature to adjust blood flow to meet metabolic requirements. At the level of gene transcription, the responses are mediated by hypoxia-inducible factor (HIF) the stability of which is controlled by the HIF prolyl 4-hydroxylase-2 (PHD2). In the lungs hypoxia results in vasoconstriction, however, the pathophysiological relevance of PHD2 in the major arterial cell types; endothelial cells (ECs) and arterial smooth muscle cells (aSMCs) in the adult vasculature is incompletely characterized. Here, we investigated PHD2-dependent vascular homeostasis utilizing inducible deletions of PHD2 either in ECs (Phd2∆ECi) or in aSMCs (Phd2∆aSMC). Cardiovascular function and lung pathologies were studied using echocardiography, Doppler ultrasonography, intraventricular pressure measurement, histological, ultrastructural, and transcriptional methods. Cell intrinsic responses were investigated in hypoxia and in conditions mimicking hypertension-induced hemodynamic stress. Phd2∆ECi resulted in progressive pulmonary disease characterized by a thickened respiratory basement membrane (BM), alveolar fibrosis, increased pulmonary artery pressure, and adaptive hypertrophy of the right ventricle (RV). A low oxygen environment resulted in alterations in cultured ECs similar to those in Phd2∆ECi mice, involving BM components and vascular tone regulators favoring the contraction of SMCs. In contrast, Phd2∆aSMC resulted in elevated RV pressure without alterations in vascular tone regulators. Mechanistically, PHD2 inhibition in aSMCs involved  actin polymerization -related tension development via activated cofilin. The results also indicated that hemodynamic stress, rather than PHD2-dependent hypoxia response alone, potentiates structural remodeling of the extracellular matrix in the pulmonary microvasculature and respiratory failure.
The 2020 national seismic hazard model for the United Kingdom
Authors: Mosca I. , Sargeant S. , Baptie B. , Musson R. M. W. , Pharaoh T. C.
Published: January 2022

We present updated seismic hazard maps for the United Kingdom (UK) intended for use with the National Annex for the revised edition of Eurocode 8. The last national maps for the UK were produced by Musson and Sargeant (Eurocode 8 seismic hazard zoning maps for the UK. British Geological Survey Report CR/07/125, United Kingdom, 2007). The updated model uses an up-to-date earthquake catalogue for the British Isles, for which the completeness periods have been reassessed, and a modified source model. The hazard model also incorporates some advances in ground motion modelling since 2007, including host-to-target adjustments for the ground motion models selected in the logic tree. For the first time, the new maps are provided for not only peak ground acceleration (PGA) but also spectral acceleration at 0.2 s (SA0.2s) and 1.0 s for 5% damping on rock (time-averaged shear wave velocity for the top 30 m Vs30 ≥ 800 m/s) and four return periods, including 475 and 2475 years. The hazard in most of the UK is generally low and increases slightly in North Wales, the England–Wales border region, and western Scotland. A similar spatial variation is observed for PGA and SA0.2sbut the effects are more pronounced for SA0.2s. Hazard curves, uniform hazard spectra, and disaggregation analysis are calculated for selected sites. The new hazard maps are compared with the previous 2007 national maps and the 2013 European hazard maps (Woessner et al. in Bull Earthq Eng 13:3553–3596, 2015). There is a slight increase in PGA from the 2007 maps to this work; whereas the hazard in the updated maps is lower than indicated by the European maps.
The Role of Moral Foundations, Anticipated Guilt and Personal Responsibility in Predicting Anti-consumption for Environmental Reasons
Authors: Culiberg Barbara , Cho Hichang , Kos Koklic Mateja , Zabkar Vesna
Published: January 2022

In response to the growing importance of environmental issues, more and more consumers are turning to anti-consumption by reducing, rejecting, or avoiding consumption. Covering the intersection of sustainable consumption and anti-consumption, previous studies relied on socio-cognitive models to explain this decision. In order to extend their findings, we consider the moral and emotional perspectives to examine reducing consumption for environmental reasons in a particular context, i.e. air travel. It is against this backdrop that we propose a conceptual model that includes moral foundations as the main antecedent, followed by anticipated guilt and personal responsibility, while intention to reduce consumption (i.e. air travel) for environmental reasons, positive word of mouth about reducing air travel (WOM) and environmental activism represent the outcomes. The proposed model is tested on a sample of 511 respondents from a UK online consumer panel. Our results confirm the importance of moral foundations, anticipated guilt and personal responsibility and their interplay in the prediction of intention to reduce consumption for environmental reasons. Anticipated guilt influences WOM, while personal responsibility influences activism. In addition, intentions to reduce consumption for environmental reasons have a positive impact on WOM and environmental activism. There are several implications for public policy makers and NGOs that fight against climate change that derive from these findings, as well as research opportunities for academics interested in this topic.
Mobility of kinetochore proteins measured by FRAP analysis in living cells
Authors: Watanabe Reito , Hirano Yasuhiro , Hara Masatoshi , Hiraoka Yasushi , Fukagawa Tatsuo
Published: January 2022

The kinetochore is essential for faithful chromosome segregation during mitosis and is assembled through dynamic processes involving numerous kinetochore proteins. Various experimental strategies have been used to understand kinetochore assembly processes. Fluorescence recovery after photobleaching (FRAP) analysis is also a useful strategy for revealing the dynamics of kinetochore assembly. In this study, we introduced fluorescence protein-tagged kinetochore protein cDNAs into each endogenous locus and performed FRAP analyses in chicken DT40 cells. Centromeric protein (CENP)-C was highly mobile in interphase, but immobile during mitosis. CENP-C mutants lacking the CENP-A-binding domain became mobile during mitosis. In contrast to CENP-C, CENP-T and CENP-H were immobile during both interphase and mitosis. The mobility of Dsn1, which is a component of the Mis12 complex and directly binds to CENP-C, depended on CENP-C mobility during mitosis. Thus, our FRAP assays provide dynamic aspects of how the kinetochore is assembled.
Bidirectional Relationship Between Family Accommodation and Youth Anxiety During Cognitive-Behavioral Treatment
Authors: Bertelsen Thomas B. , Himle Joeseph A. , Håland Åshild Tellefsen
Published: January 2022

Family accommodation is associated with an increase in anxiety and has recently received attention as a target for intervention for youth anxiety. Existing theories posit that the increase in family accommodation increases youth anxiety and can attenuate the effect of psychotherapy. However, the directionality between family accommodation and youth anxiety has not been investigated. A cross-lagged cross-panel design was used to assess accommodation and anxiety for 10 sessions for 73 youths with an anxiety disorder, who were receiving cognitive-behavioral therapy. The analysis revealed a bidirectional relationship, such that to some extent previous session family accommodation increased youth anxiety symptoms (β = 0.11, 95% CI [0.06, 0.17]), but to an even greater extent previous session youth-rated anxiety symptoms increased family accommodation (β = 0.23, 95% CI [0.08, 0.38]). Family accommodation is an important target for reducing youth anxiety but should be addressed simultaneously as interventions directly targeting youth anxiety.
Efficacy of apatinib combined with FOLFIRI in the first-line treatment of patients with metastatic colorectal cancer
Authors: Rong Xuetong , Liu Haiyi , Yu Hongmei , Zhao Jian , Wang Jie , Wang Yusheng
Published: January 2022

Objective. To evaluate the efficacy and safety of apatinib combined with FOLFIRI in the first-line treatment of advanced metastatic colorectal cancer (mCRC) and explore potential factors of efficacy. Methods. Twenty mCRC patients treated at Affiliated Cancer Hospital of Shanxi Medical University from March 2017 to March 2019 were included according to the enrolment criteria. They provided informed consent and were treated with apatinib combined with FOLFIRI according to the scheduled regimen until disease progression or unacceptable toxicity occurred. The primary endpoint was OS. The secondary endpoints included PFS, ORR, DCRand safety. OS and PFS were calculated using Kaplan–Meier curves. Univariate and multivariate Cox regression analyses were used to evaluate independent prognostic factors of OS and PFS. R was used to determine cut-off values for biochemical indicators. Forest maps were drawn for Cox univariate results and the relationships between NLR and ECOG, which were significant in univariate analysis, and OS were represented by Kaplan–Meier curves. Results. The median OS and PFS were 16.135 months (95% CI: 9.211–22.929) and 6 months (95% CI: 5.425–6.525). Multivariate Cox analysis showed that NLR and CEA were independent prognostic factors. The most common grade 3–4 adverse events were hypertension, diarrhoea, increased alkaline phosphatase, decreased leukocytes and decreased neutrophils. Conclusion. Apatinib combined with FOLFIRI for the first-line treatment of advanced unresectable mCRC showed good efficacy and safety. The baseline NLR was predictive of efficacy, and a low baseline NLR (HR: 0.2895, P = 0.0084) was associated with improved OS.

Clinical Research Registration Number: ChiCTR1800015308.
Has the European Union entered a bioeconomy transition? Combining an output-based approach with a shift-share analysis
Authors: Ronzon Tévécia , Iost Susanne , Philippidis George
Published: January 2022

The bioeconomy is a collective of activities charged with the production of biologically renewable resources or ‘biomass’ (e.g. agriculture, forestry), its diverse application (e.g. food, textiles, construction, chemicals) and subsequent reuse (e.g. compositing, waste management). Since the European Union (EU) launched its bioeconomy strategy in 2012, further bioeconomy policy initiatives have proliferated at regional, national and pan-European levels. Moreover, the EU Green Deal announced in 2019 targets a transition towards a low-carbon sustainable model of growth, food and energy security, biodiversity and natural resource management, where it is envisaged that the bioeconomy will play a key role. Despite a paucity of available data, the surge in policy interest has triggered the need for evidence-based monitoring of bioeconomy sectors and the efficient tailoring of policy support. Thus, on a Member State (MS) basis for the period 2008–2017, we (1) adopt an ‘output-based’ approach to construct a panel data of performance indicators and (2) characterise the sources of growth and transitional stage of the bioeconomy. Results reveal that the bioeconomy has maintained its relative importance within the total EU27 economy. At the EU level, agriculture and the food industry have played a key role in driving a transition in the primary and industrial bioeconomy sectors due to their significant labour productivity-enhancing impact. Four Northern MS exhibit a bioeconomy transition by modernising their bioeconomy activities and operating structural changes. Other Northern and Western EU MS are still in the early stages of a transition, whilst in Eastern and Central Europe, such a transition remains elusive.
Third-party vocal intervention in the proboscis monkey (Nasalis larvatus)
Authors: Scheumann Marina , Röper Kathrin M. , Nathan Senthilvel K. S. S. , Goossens Benoit
Published: January 2022

Vocal intervention is a triadic social interaction, where a third party responds vocally to a conflict between group members, minimizing the costs of aggression in response to the intervention. Because there is little information on vocal third-party intervention in nonhuman mammals, we investigated whether adult male proboscis monkeys use the bray vocalization as a vocal third-party intervention signal to intervene in intragroup conflicts. First, we audio-recorded 1,811 vocalizations from 17 free-ranging proboscis monkey groups in the Lower Kinabatangan Wildlife Sanctuary, analyzing 378 vocal responses of the adult male to agonistic vocal exchanges (shrieks) of group members. Second, we video- and audio-recorded five habituated groups in the Labuk Bay Proboscis Monkey Sanctuary investigating the context of these vocalizations and the conflict dyads evoking vocal support. We found that adult males of one-male/multifemale groups mainly uttered bray vocalizations, whereas females, immatures, and infants uttered shrieks in intragroup conflicts or in response to other animal species. The adult male uttered significantly more often brays after agonistic shrieks than expected based on the overall occurrence of brays. Brays ended 65% of agonistic conflicts, which were accompanied by vocalizations of the conflict partners and occurred more often after conflicts between females than between offspring. This suggests that the bray functions as a vocal third-party intervention signal for intragroup conflict resolution. We suggest that living in the high canopies of the tropical rainforest might restrict direct access to conflict partners and prevent physical intervention, favoring the evolution of the bray as a third-party vocal intervention signal.
Quenched Decay of Correlations for One-dimensional Random Lorenz Maps
Authors: Larkin Andrew
Published: January 2022

We study rates of mixing for small random perturbations of one-dimensional Lorenz maps. Using a random tower construction, we prove that, for Hölder observables, the random system admits exponential rates of quenched correlation decay.
G-protein coupled receptor 34 regulates the proliferation and growth of LS174T cells through differential expression of PI3K subunits and PTEN
Authors: Zuo Bo , Wu Na , Yang Shen , Zhong Zhaohui , Li Mei , Yu Xin , Liu Yulan , Yu Weidong
Published: January 2022

Purpose

G-protein coupled receptor (GPR 34) has been found to play important roles in some cancers and regulates the proliferation, apoptosis, and migration of these cancer cells. However, the mechanisms underlying how GPR34 functions to regulate growth and proliferation of colorectal cancer cells remains to be clarified.

Methods

We employed stable GPR34 knockdown LS174T cell models, GPR34 Mab blocking, a CCK-8 kit, and a colony formation assay to characterize the effect of GPR34 on the proliferation of LS174T in vitro and xenograft tumor growth in vivo. The mRNA level of GPR34 was detected by RT-PCR in tumor tissues and adjacent normal tissues from 34 CRC patients.

Results

Based on RT-PCR results, GPR34 exhibited high level in tumor samples compared with adjacent normal samples. Increased expression of GPR34 is more associated with poor prognosis of CRC as shown in The Cancer Genome Atlas (TCGA) dataset by Kaplan–Meier survival analysis. Furthermore, we showed that GPR34 knockdown inhibited the proliferation of LS174T colon cancer cells and related xenograft tumor growth. Searching for the distinct molecular mechanism, we identified several contributors to proliferation of LS174T colon cancer cells: PI3K subunits/PTEN, PDK1/AKT, and Src/Raf/Ras/ERK. GPR34 knockdown inhibited the proliferation of LS174T cells by upregulating expression of PTEN, and downregulating expression of PI3K subunits p110-beta.

Conclusion

Our findings provide direct evidence that GPR34 regulates the proliferation of LS174T cells and the growth of LS174T tumor xenografts by regulating different pathways. High expression of GPR34 mRNA could then be used to predict poor prognosis of CRC.
Predicting the impact of online news articles – is information necessary?
Authors: Preiss Judita
Published: January 2022

We exploit the Twitter platform to create a dataset of news articles derived from tweets concerning COVID-19, and use the associated tweets to define a number of popularity measures. The focus on (potentially) biomedical news articles allows the quantity of biomedically valid information (as extracted by biomedical relation extraction) to be included in the list of explored features. Aside from forming part of a systematic correlation exploration, the features – ranging from the semantic relations through readability measures to the article’s digital content – are used within a number of machine learning classifier and regression algorithms. Unsurprisingly, the results support that for more complex articles (as determined by a readability measure) more sophisticated syntactic structure may be expected. A weak correlation is found with information within an article suggesting that other factors, such as numbers of videos, have a notable impact on the popularity of a news article. The best popularity prediction performance is obtained using a random forest machine learning algorithm, and the feature describing the quantity of biomedical information is in the top 3 most important features in almost a third of the experiments performed. Additionally, this feature is found to be more valuable than the widely used named entity recognition.
$$360^{\circ }$$ real-time and power-efficient 3D DAMOT for autonomous driving applications
Authors: Gómez-Huélamo Carlos , Del Egido Javier , Bergasa Luis Miguel , Barea Rafael , López-Guillén Elena , Araluce Javier , Antunes Miguel
Published: January 2022

Autonomous Driving (AD) promises an efficient, comfortable and safe driving experience. Nevertheless, fatalities involving vehicles equipped with Automated Driving Systems (ADSs) are on the rise, especially those related to the perception module of the vehicle. This paper presents a real-time and power-efficient 3D Multi-Object Detection and Tracking (DAMOT) method proposed for Intelligent Vehicles (IV) applications, allowing the vehicle to track $$360^{\circ }$$

360
∘

surrounding objects as a preliminary stage to perform trajectory forecasting to prevent collisions and anticipate the ego-vehicle to future traffic scenarios. First, we present our DAMOT pipeline based on Fast Encoders for object detection and a combination of a 3D Kalman Filter and Hungarian Algorithm, used for state estimation and data association respectively. We extend our previous work ellaborating a preliminary version of sensor fusion based DAMOT, merging the extracted features by a Convolutional Neural Network (CNN) using camera information for long-term re-identification and obstacles retrieved by the 3D object detector. Both pipelines exploit the concepts of lightweight Linux containers using the Docker approach to provide the system with isolation, flexibility and portability, and standard communication in robotics using the Robot Operating System (ROS). Second, both pipelines are validated using the recently proposed KITTI-3DMOT evaluation tool that demonstrates the full strength of 3D localization and tracking of a MOT system. Finally, the most efficient architecture is validated in some interesting traffic scenarios implemented in the CARLA (Car Learning to Act) open-source driving simulator and in our real-world autonomous electric car using the NVIDIA AGX Xavier, an AI embedded system for autonomous machines, studying its performance in a controlled but realistic urban environment with real-time execution (results).
Final results of the Choroid Plexus Tumor study CPT-SIOP-2000
Authors: Wolff Johannes E. , Van Gool Stefaan W. , Kutluk Tezer , Diez Blanca , Kebudi Rejin , Timmermann Beate , Garami Miklos , Sterba Jaroslav , Fuller Gregory N. , Bison Brigitte , Kordes Uwe R.
Published: January 2022

Introduction

Standards for chemotherapy against choroid plexus tumors (CPT) have not yet been established.

Methods

CPT-SIOP-2000 (NCT00500890) was an international registry for all CPT nesting a chemotherapy randomization for high-risk CPT with Carboplatin/Etoposide/Vincristine (CarbEV) versus Cyclophosphamide/Etoposide/Vincristine (CycEV). Patients older than three years were recommended to receive irradiation: focal fields for non-metastatic CPC, incompletely resected atypical choroid plexus papilloma (APP) or metastatic choroid plexus papilloma (CPP); craniospinal fields for metastatic CPC/APP and non-responsive CPC. High risk was defined as choroid plexus carcinoma (CPC), incompletely resected APP, and all metastatic CPT. From 2000 until 2010, 158 CPT patients from 23 countries were enrolled.

Results

For randomized CPC, the 5/10 year progression free survival (PFS) of patients on CarbEV (n = 20) were 62%/47%, respectively, compared to 27%/18%, on CycEV (n = 15), (intention-to-treat, HR 2.6, p = 0.032). Within the registry, histological grading was the most influential prognostic factor: for CPP (n = 55) the 5/10 year overall survival (OS) and the event free survival (EFS) probabilities were 100%/97% and 92%/92%, respectively; for APP (n = 49) 96%/96% and 76%/76%, respectively; and for CPC (n = 54) 65%/51% and 41%/39%, respectively. Without irradiation, 12 out of 33 patients with CPC younger than three years were alive for a median of 8.52 years. Extent of surgery and metastases were not independent prognosticators.

Conclusions

Chemotherapy for Choroid Plexus Carcinoma is feasible and effective. CarbEV is superior to CycEV. A subset of CPC can be cured without irradiation.
Photosynthetic traits of Australian wild rice (Oryza australiensis) confer tolerance to extreme daytime temperatures
Authors: Phillips Aaron L. , Scafaro Andrew P. , Atwell Brian J.
Published: January 2022

Key message

A wild relative of rice from the Australian savannah was compared with cultivated rice, revealing thermotolerance in growth and photosynthetic processes and a more robust carbon economy in extreme heat.

Above ~ 32 °C, impaired photosynthesis compromises the productivity of rice. We compared leaf tissues from heat-tolerant wild rice (Oryza australiensis) with temperate-adapted O. sativa after sustained exposure to heat, as well as diurnal heat shock. Leaf elongation and shoot biomass in O. australiensis were unimpaired at 45 °C, and soluble sugar concentrations trebled during 10 h of a 45 °C shock treatment. By contrast, 45 °C slowed growth strongly in O. sativa. Chloroplastic CO2 concentrations eliminated CO2 supply to chloroplasts as the basis of differential heat tolerance. This directed our attention to carboxylation and the abundance of the heat-sensitive chaperone Rubisco activase (Rca) in each species. Surprisingly, O. australiensis leaves at 45 °C had 50% less Rca per unit Rubisco, even though CO2 assimilation was faster than at 30 °C. By contrast, Rca per unit Rubisco doubled in O. sativa at 45 °C while CO2 assimilation was slower, reflecting its inferior Rca thermostability. Plants grown at 45 °C were simultaneously exposed to 700 ppm CO2 to enhance the CO2 supply to Rubisco. Growth at 45 °C responded to CO2 enrichment in O. australiensis but not O. sativa, reflecting more robust carboxylation capacity and thermal tolerance in the wild rice relative.
Bio, psycho, or social: supervised machine learning to classify discursive framing of depression in online health communities
Authors: Németh Renáta , Máté Fanni , Katona Eszter , Rakovics Márton , Sik Domonkos
Published: January 2022

Supervised machine learning on textual data has successful industrial/business applications, but it is an open question whether it can be utilized in social knowledge building outside the scope of hermeneutically more trivial cases. Combining sociology and data science raises several methodological and epistemological questions. In our study the discursive framing of depression is explored in online health communities. Three discursive frameworks are introduced: the bio-medical, psychological, and social framings of depression. ~80 000 posts were collected, and a sample of them was manually classified. Conventional bag-of-words models, Gradient Boosting Machine, word-embedding-based models and a state-of-the-art Transformer-based model with transfer learning, called DistilBERT were applied to expand this classification on the whole database. According to our experience ‘discursive framing’ proves to be a complex and hermeneutically difficult concept, which affects the degree of both inter-annotator agreement and predictive performance. Our finding confirms that the level of inter-annotator disagreement provides a good estimate for the objective difficulty of the classification. By identifying the most important terms, we also interpreted the classification algorithms, which is of great importance in social sciences. We are convinced that machine learning techniques can extend the horizon of qualitative text analysis. Our paper supports a smooth fit of the new techniques into the traditional toolbox of social sciences.
Fission theories of Original Guilt
Authors: Effingham Nikk
Published: January 2022

One reading of the Doctrine of Original Sin has it that we are guilty of a sin committed by Adam, thousands of years ago. Fission theorists account for this by saying that Adam fissioned after he sinned and that each of us is one of his ‘fission successors’. This paper recaps the current discussion in the literature about this theory, arguing that the proposed version does not work for reasons already raised by Rea and Hudson. I then introduce a new version of fission theory that avoids the Rea-Hudson objection.
A Lepskiĭ-type stopping rule for the covariance estimation of multi-dimensional Lévy processes
Authors: Papagiannouli Katerina
Published: January 2022

We suppose that a Lévy process is observed at discrete time points. Starting from an asymptotically minimax family of estimators for the continuous part of the Lévy Khinchine characteristics, i.e., the covariance, we derive a data-driven parameter choice for the frequency of estimating the covariance. We investigate a Lepskiĭ-type stopping rule for the adaptive procedure. Consequently, we use a balancing principle for the best possible data-driven parameter. The adaptive estimator achieves almost the optimal rate. Numerical experiments with the proposed selection rule are also presented.
The attributable mortality of new-onset acute kidney injury among critically ill patients: a propensity-matched analysis based on a multicentre prospective cohort study
Authors: Jiang Yi-Jia , Xi Xiu-Ming , Jia Hui-Miao , Zheng Xi , Wang Mei-Ping , Li Wen-Xiong
Published: January 2022

Purpose

This study aimed to evaluate the attributable mortality of new-onset acute kidney injury (AKI).

Methods

The data in the present study were derived from a multi-center, prospective cohort study in China that was performed at 18 Chinese ICUs. A propensity-matched analysis was performed between matched patients with and without AKI selected from all eligible patients to estimate the attributable mortality of new-onset AKI.

Results

A total of 2872 critically ill adult patients were eligible. The incidence of new-onset AKI was 29.1% (n = 837). After propensity score matching, 788 patients with AKI were matched 1:1 with 788 controls (patients without AKI). Thirty-day mortality was significantly higher among the patients with AKI than among their matched controls (25.5% versus 17.4%, p < 0.001). Subgroup analysis in terms of AKI classification showed that there was no significant difference (p = 0.509) in 30-day mortality between patients with stage 1 AKI and their matched controls. The attributable mortality values of stage 2 and stage 3 AKI were 12.4% [95% confidence interval (CI) 2.6–21.8%, p = 0.013] and 16.1% (95% CI 8.2–23.8%, p < 0.001), respectively. The attributable mortality of persistent AKI was 15.7% (95% CI 8.8–22.4%, p = 0.001), while no observable difference in 30-day mortality was identified between transient AKI patients and their matched non-AKI controls (p = 0.229).

Conclusion

The absolute excess 30-day mortality that is statistically attributable to new-onset AKI is substantial (8.1%) among general ICU patients. However, neither stage 1 AKI nor transient AKI increases 30-day mortality.
High concentrations of pharmaceuticals emerging as a threat to Himalayan water sustainability
Authors: Quincey Duncan J. , Kay Paul , Wilkinson John , Carter Laura J. , Brown Lee E.
Published: January 2022

The sixth UN Sustainable Development Goal, Clean Water and Sanitation, directly underpins other goals of Health, Life in Water and Sustainable Cities. We highlight that poor sanitation, exemplified through some of the highest concentrations of pharmaceuticals ever detected in rivers, will amplify societal and environmental stress where climate-induced reductions in flow are predicted. Rapidly growing urban centres with inadequate water treatment works will need to prioritise water quality improvement before supply reductions become a reality. For 23 river locations within Kathmandu City and the Annapurna region, Nepal, we show the presence of 28 of 35 monitored human-use pharmaceuticals. Concentrations of antibiotics measured in this sampling campaign in both Kathmandu City (sulfamethazine, metronidazole and ciprofloxacin) and rural locations (ciprofloxacin) are in excess of predicted no effect concentrations, suggesting these sites are at risk of proliferating antimicrobial resistance as well as affecting other ecotoxicological endpoints. It is anticipated that climate-induced reductions in flow combined with contaminated river systems will amplify future societal and environmental stress.
Associations between sociodemographic factors, health spending, disease burden, and life expectancy of older adults (70 + years old) in 22 countries in the Western Pacific Region, 1995–2019: estimates from the Global Burden of Disease (GBD) Study 2019
Authors: Molassiotis Alex , Kwok Stephen W. H. , Leung Angela Y. M. , Tyrovolas Stefanos
Published: January 2022

The needs of the aging populations are putting increasing burden on healthcare particularly in the Western Pacific Region (WPR), which is the home of aging economies such as Hong Kong and Japan alongside rapid increases in older people in low- and middle-income countries (LMICs). However, little is known about the associations between sociodemographic factors, disease burden, and life expectancy in WPR. The current study conducted secondary analysis on the Global Burden of Disease (GBD) estimates of populations aged 70 years and older in WPR countries between 1995 and 2019. Correlation tests, linear mixed regressions and generalized additive mixture models were run to examine the associations of interest. Unsupervised machine learning was conducted to segment the data automatically, at cluster analysis. The sociodemographic development index was found an important factor to the disease burden in terms of Years Lived with Disability (YLD) rate and mortality rate among older adults. YLD rate and mortality rate of non-communicable diseases (NCDs) attributable to different risk groups were significant contributors to higher expectation of Lost Healthy Years (LHE) and shorter life expectancy respectively in the context of rising YLD rate, particularly in LMICs. Three clusters of countries with similar characteristics were identified. NCDs were the most significant contributors to shorter life expectancy within which the time living in poor health did not improve. Better management of NCDs in aging populations should be emphasized in all countries. The country clusters may help in a more regional strategic planning.
COVID-19 and the Wuhan Diary –how does the overseas Chinese community react to group criticism?
Authors: Wang Mei , Rieger Marc Oliver
Published: January 2022

We conduct an online survey to explore how Chinese people living in Germany perceive and react to group criticism in the context of the debate on the Wuhan Diary, a chronicle about life during the lockdown in Wuhan. We find that the majority rating of the book is a lukewarm “neither like nor dislike.” Most participants are open to criticism in principle and do not agree that the book only spreads so-called “negative-energy”. However, many participants were skeptical about the objectivity of the book and concerned about its potential use by so-called anti-China forces, even though the degree of blind patriotism is relatively low in our sample. The factors influencing the book’s evaluation are intriguing: perceived Western sentiment, media exposure and uncritical patriotism all affect COVID-19-related conspiracy beliefs, which in turn lead to a more negative evaluation of the book. A cluster analysis reveals two groups which differ in terms of properties like blind patriotism, belief in certain conspiracies, and also demographic parameters. Our results shed light on identity politics, motivated beliefs, and collective narcissism.