bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence Estimates and Risk Factors of Anxiety among Healthcare Workers in Jordan over One Year of the COVID-19 Pandemic: A Cross-Sectional Study
Authors: Yassin Ahmed , Al-Mistarehi Abdel-Hameed , El-Salem Khalid , Karasneh Reema A. , Al-Azzam Sayer , Qarqash Aref A. , Khasawneh Aws G. , Zein Alaabdin Anas M. , Soudah Ola
Published: February 2022

This study investigates the changes in prevalence estimates, severity, and risk factors of anxiety among healthcare workers (HCWs) over the first year of the COVID-19 pandemic. A survey was distributed among HCWs using snowball sampling, collecting their socio-demographics, occupation, and anxiety symptoms as measured by the Generalized Anxiety Disorder-7 (GAD-7) scale. It was distributed one month after the pandemic’s onset in Jordan between 15 and 30 April 2020 (onset group) and after one year between 15 and 30 March 2021 (one-year group). A total of 422 HCWs were included (211 in each group). The one-year group reported a higher risk of GAD (30.8% vs. 16.6%; p = 0.001), a higher mean (SD) GAD-7 score (7.94 (5.29) vs. 6.15 (4.15); p < 0.001), and more severe symptoms (p = 0.003). Univariate analyses showed that participants who were younger, women, unmarried, had lower monthly incomes, underwent testing for COVID-19, had higher contact with COVID-19 patients, did not receive special education, and were unsatisfied with the institutional COVID-19 preparedness scored higher on the GAD-7 scale and had more severe symptoms than their counterparts in both groups. Unlike the onset group, occupation as a physician, COVID-19 infection history, and perception of remarkable changes in work were associated with higher anxiety scores and severity among the one-year group. The COVID-19 vaccine was a relative protective action. Logistic regression analyses showed that the female gender was a risk factor for developing GAD at the pandemic onset, while poor satisfaction with institutional preparedness was a significant GAD risk factor in the one-year group. Low monthly income and lack of special education were the shared risk factors for GAD in both groups. This study reveals a significant rise in anxiety among HCWs over a year of the COVID-19 pandemic and shows the vulnerable sub-groups who likely need psychological interventions.
The Pattern of Hemoglobin A1C Trajectories and Risk of Herpes Zoster Infection: A Follow-Up Study
Authors: Pan Bo-Lin , Chou Chia-Pei , Huang Kun-Siang , Bin Pin-Jie , Luo Kuei-Hau , Chuang Hung-Yi
Published: February 2022

To investigate the risks of herpes zoster (HZ) infection among heterogeneous HbA1C trajectories of patients with newly diagnosed type 2 diabetes, this cohort study used data from the Chang Gung Research Database (CGRD), from the 10-year period of 1 January 2007 to 31 December 2017. We applied group-based trajectory modeling (GBTM) to identify the patterns of HbA1C trajectories, and multiple Cox proportional hazards regressions were used to estimate the hazard ratio (HR) for the risk of HZ infection with adjustment of age, sex, and comorbidities. This study enrolled 121,999 subjects to perform the analysis. The GBTM identified four HbA1C trajectories: ‘good control’ (58.4%), ‘high decreasing’ (8.9%), ‘moderate control’ (25.1%), and ‘poor control’ (7.6%) with the mean HbA1C of 6.7% (50 mmol/mol), 7.9% (63 mmol/mol), 8.4% (68 mmol/mol), and 10.7% (93 mmol/mol) respectively. The risk of HZ was significantly higher in the poor control with an HR = 1.44 (95% CI 1.26–1.64) after adjustment for confounders and comorbidities. The risk of HZ infection for the high decreasing group (initially poor then rapidly reaching optimal control) was nonsignificant compared to the good control group. In conclusion, the patients with poor glycemic control (mean HbA1C = 10.7%) had the highest risk of HZ infection. The patients with initial hyperglycemia then reaching optimal control could have a lower risk of HZ infection.
Independence and Sex Differences in Physical Activity and Sedentary Behavior Trends from Middle Adolescence to Emerging Adulthood: A Latent Class Growth Curve Analysis
Authors: Luo Yan , Zhong Juan
Published: February 2022

The purpose of this study was to examine the relationship and sex differences in the growth patterns of moderate-to-vigorous physical activity (MVPA) and sedentary behavior (SB) from middle adolescence (around age 15) to emerging adulthood (18–29 years old). We used the secondary data from the National Longitudinal Study of Adolescent Health’s (Add Health) publicly accessible database. MVPA and SB were assessed four times (1995, 1996, 2000–2001, and 2008–2009) for a total of 681 individuals from middle adolescence to emerging adulthood. Latent class growth modeling was utilized to identify heterogeneous growth patterns in MVPA and SB. Chi-square tests were used to assess group dependence and sex differences in MVPA and SB growth patterns. Seven quadratic growth patterns for MVPA and two linear growth patterns for SB were found. Both MVPA and SB growth patterns exhibited slow or rapid rate of change at different periods of adolescence and emerging adulthood. MVPA growth patterns included: decline (slow)-and-rise (rapid), rise-and-decline (both rapid), decline-and-rise (both rapid), consistently low, consistently high, decline (rapid)-and-decline (slow), and decline (rapid)-and-rise (slow). SB growth patterns included: consistently low (slow decline) and consistently high (rapid decline). While women were more likely to be involved in consistently low group for MVPA and consistently low (slow decline) group for SB, men were more likely to be involved in rise-and-decline (both rapid) group, consistently high group, and decline (rapid)-and-rise (slow) group for MVPA and consistently high (rapid decline) group for SB. SB growth patterns were independent of MVPA growth patterns. SB should not be assumed to decrease as a result of MVPA intervention. Treatment of MVPA should prioritize adolescence over emerging adulthood, with an emphasis on preventing men’s MVPA levels from decreasing in emerging adulthood and increasing women’s overall MVPA levels.
Quality of Life among Next of Kin of Frail Older People in Nursing Homes: An Interview Study after an Educational Intervention concerning Palliative Care
Authors: Ahlström Gerd , Rosén Helena , Persson Eva I.
Published: February 2022

One cornerstone of palliative care is improving the family’s quality of life (QoL). The principles of palliative care have not been sufficiently applied in nursing homes. The aim of this study was to investigate the experiences of QoL of next of kin of frail older persons in nursing homes after an educational intervention concerning palliative care. This qualitative interview study with 37 next of kin used an abductive design with deductive and inductive content analysis. The deductive analysis confirmed the three themes of QoL from the study before the implementation: (1) orientation to the new life-situation, (2) challenges in the relationship, and (3) the significance of the quality of care in the nursing home. The inductive analysis resulted in the sub-theme “Unspoken palliative care”. Being the next of kin of an older person living in a nursing home can be distressing despite round-the-clock care, so staff need to apply a more explicitly palliative care perspective. Future research needs to evaluate the influence of meaning-focused coping on next of kin’s QoL and integrate this knowledge in psychosocial interventions. Clinical Trial Database for Clinical Research: KUPA project NCT02708498.
Negative Self-Assessment of Health in Women: Association with Sociodemographic Characteristics, Physical Inactivity and Multimorbidity
Authors: Santos Thays Angélica de Pinho , Guimarães Rafael Alves , Pagotto Valéria , Aredes Natália Del’ Angelo , Siqueira Isabela Silva Levindo de , Rocha Suiany Dias , Carrijo Clarissa Irineu de Sousa , Rosso Claci Fátima Weirich
Published: February 2022

Introduction: Women present a higher prevalence of negative self-assessment of health (NSAH) when compared to men. However, there is a gap in the literature of factors associated with NSAH in women from developing countries such as Brazil. In addition, few studies have assessed the magnitude of the association between multimorbidity and NSAH in this population. Thus, the aim of this study was to evaluate the association between NSAH and sociodemographic characteristics, lifestyle and multimorbidity in women from the Midwest region of Brazil. Methods: A study based on data from the National Health Survey, a household survey that investigated health situation, lifestyle and risk factors for chronic diseases in the adult population of Brazil, was held. Sampling was performed in multiple stages. The selected women answered a standardized questionnaire on sociodemographic data, self-assessment of health and potential determinants. Poisson regression was used to analyze the association between NSAH and sociodemographic characteristics, lifestyle and multimorbidity. A significance level of 0.05% was established. Results: The study included 4233 women. The prevalence of NSAH found was 6.0% (95% Confidence Interval [95% CI]: 5.1–7.0%). There was an association between NSAH and advancing age, low schooling, physical inactivity and multimorbidity. Furthermore, there was an association between NSAH and diseases/disorders such as chronic back pain, systemic arterial hypertension, mental disorders, depression, cardiovascular diseases, stroke, cancer, hypercholesterolemia and diabetes mellitus. Conclusion: The prevalence of NSAH was low. A strong association was found between this variable and multimorbidity. In addition, increased age, low schooling and physical inactivity were predictors of NSAH in women.
Sulfidic Habitats in the Gypsum Karst System of Monte Conca (Italy) Host a Chemoautotrophically Supported Invertebrate Community
Authors: Nicolosi Giuseppe , Galdenzi Sandro , Messina Maria Anna , Miller Ana Z. , Petralia Salvatore , Sarbu Serban M. , Isaia Marco
Published: February 2022

The great diversity of the invertebrate community thriving in the deepest sections of the gypsum karst system of the Monte Conca sinkhole (Sicily, Italy) suggests the existence of a complex food web associated with a sulfidic pool and chemoautotrophic microbial activity. To shed light on the peculiarity of this biological assemblage, we investigated the species composition of the invertebrate community and surveyed trophic interactions by stable isotope analysis. The faunal investigation conducted by visual censuses and hand sampling methods led to the discovery of a structured biological assemblage composed of both subterranean specialized and non-specialized species, encompassing all trophic levels. The community was remarkably diverse in the sulfidic habitat and differed from other non-sulfidic habitats within the cave in terms of stable isotope ratios. This pattern suggests the presence of a significant chemoautotrophic support by the microbial communities to the local food web, especially during the dry season when the organic input from the surface is minimal. However, when large volumes of water enter the cave due to local agricultural activities (i.e., irrigation) or extreme precipitation events, the sulfidic habitat of the cave is flooded, inhibiting the local autotrophic production and threatening the conservation of the entire ecosystem.
Heavy Metals in Harvested Rainwater Used for Domestic Purposes in Rural Areas: Yatta Area, Palestine as a Case Study
Authors: Anabtawi Fathi , Mahmoud Nidal , Al-Khatib Issam A. , Hung Yung-Tse
Published: February 2022

Rainwater harvesting is considered one of the most important water resources in the Palestinian countryside. In this research, the study area chosen for the study was Yatta town in Hebron city. 75 water samples were collected from 74 cisterns in a number of neighborhoods in Yatta, and a structured household survey was conducted with the same households where the water samples were collected. Statistical analysis was made using the SPSS software. An analysis for the samples was made using ICP-MS to test the existence of a number of heavy metals, namely Pb, Cr, Mn, Co, Ni, Cu, Zn and Cd. The results were compared with the WHO and Palestinian limits for drinking water quality. Considering the metals Mn, Co, Cu and Cd, neither of the samples exceeded any of the two limits. For the metals, Pb, Cr, and Ni, two samples exceeded both limits. For the metal, Zn, one sample exceeded the WHO limit only. Sources of pollution by heavy metals of the harvested rainwater were identified by means of a questionnaire distributed to the households. The results showed that except for nickel and the water collection surface of the cistern factor, there is no direct relationship between the factors and activities that may contribute to contaminate harvested rainwater with heavy metals and the existence of heavy metals beyond local and international limits. Based on the questionnaire and literature: Possible sources of lead and zinc are the roof, storage tanks, distribution systems and plumbing; possible sources of chromium are road dust, asbestos brakes and anthropogenic activities occurring around the house; possible source of nickel is leaching from metals in contact with harvested rainwater such as pipes and fittings which are used to collect the harvested rainwater. In addition, an assessment of the potential health risks due to contamination of the harvested rainwater by heavy metals was made for all the samples that exceeded either WHO limit or the Palestinian limit or both. The Chronic Daily Intake (CDI) and the Health Risk Index (HRI) were calculated. The assessment was made for both adults and children. The results showed that all the samples are considered safe (HRI < 1), which means that there are no potential health risks for consumers.
Lung Cancer Screening in Asbestos-Exposed Populations
Authors: Markowitz Steven B.
Published: February 2022

Asbestos exposure is the most important cause of occupational lung cancer mortality. Two large randomized clinical trials in the U.S. and Europe conclusively demonstrate that annual low-dose chest CT (LDCT) scan screening reduces lung cancer mortality. Age and smoking are the chief risk factors tested in LDCT studies, but numerous risk prediction models that incorporate additional lung cancer risk factors have shown excellent performance. The studies of LDCT in asbestos-exposed populations shows favorable results but are variable in design and limited in size and generalizability. Outstanding questions include how to: (1) identify workers appropriate for screening, (2) organize screening programs, (3) inform and motivate people to screen, and (4) incorporate asbestos exposure into LDCT decision-making in clinical practice. Conclusion: Screening workers aged ≥50 years with a history of ≥5 years asbestos exposure (or fewer years given intense exposure) in combination with either (a) a history of smoking at least 10 pack-years with no limit on time since quitting, or (b) a history of asbestos-related fibrosis, chronic lung disease, family history of lung cancer, personal history of cancer, or exposure to multiple workplace lung carcinogens is a reasonable approach to LDCT eligibility, given current knowledge. The promotion of LDCT-based screening among asbestos-exposed workers is an urgent priority.
What Activities in Forests Are Beneficial for Human Health? A Systematic Review
Authors: Park Sujin , Kim Eunsoo , Kim Geonwoo , Kim Soojin , Choi Yeji , Paek Domyung
Published: February 2022

Over the past decade, clinical trials of forest-based interventions have increased, leading to their recognition as preventive medicine. However, little is known about the differences in health effects according to the activity characteristics of interventions. This study aimed to understand the types of activities and their associated health effects to identify differences in health effects between activities. PubMed, PsycINFO, Web of Science, and Scopus databases were searched, and methodological quality was assessed using Cochrane ROB2. A total of 32 randomized controlled trials (RCTs) met the eligibility criteria. Health outcomes were collected from 6264 participants aged 6–98 years, and the sample size was 12–585. The Interventions were walking (n = 21), staying (n = 7), exercise (n = 4), indirect exposure (n = 4), and the activity time was between 10 and 240 min. Overall, walking showed consistent positive health effects, and there were differences in effects on anxiety and depression, cognitive function, stress hormone, and inflammation according to the activity. However, most of the included studies had a high risk of bias, and interventions were limited to specific activities, durations, and frequencies. Although a few limitations remain, the findings in this study are of great significance in providing the basis for the design of forest-based interventions.
Distribution Dynamics of Phthalate Esters in Surface Water and Sediment of the Middle-Lower Hanjiang River, China
Authors: Dong Lei , Lin Li , Pan Xiong , Zhang Sheng , Lv Zhanao , Mi Changqing
Published: February 2022

Phthalate esters (PAEs) are endocrine-disrupting chemicals that pose potential risks to human health. Water and sediments are crucial carriers and storage media for the migration and transformation of PAEs. In this study, six congeners of PAEs were measured in water and sediment samples to elucidate their spatial distribution, congener profiles, and ecological risks in the middle-lower Hanjiang River during the wet and dry seasons. The concentration of the Σ6PAEs ranged from 592 to 2.75 × 103 ng/L with an average of 1.47 × 103 ng/L in surface water, while the concentration of the Σ6PAEs ranged from 1.12 × 103 to 6.61 × 103 ng/g with an average of 2.69 × 103 ng/g in sediments. In general, PAE concentrations were ranked as sediment > water, and dry season > wet season. DEHP and DBP were the dominant PAEs in the middle-lower Hanjiang River in surface water and sediments. SPSS analysis showed that dissolved organic carbon (DOC) in surface water was significantly correlated with the concentration of DBP, DEHP, and the ∑6PAEs, while organic matter (OM) was significantly correlated with the concentration of the ∑6PAEs in sediments. The concentrations of PAEs were irregularly distributed and varied significantly in surface water and sediments. Compared with other regions at home and abroad, the pollution levels of surface water and sediments in the middle-lower Hanjiang River were relatively low and not enough to have a negative impact on the local water’s ecological environment. However, the supervision of land-based discharge should still be strengthened.
Modification Strategies of Kapok Fiber Composites and Its Application in the Adsorption of Heavy Metal Ions and Dyes from Aqueous Solutions: A Systematic Review
Authors: Futalan Cybelle Morales , Choi Angelo Earvin S. , Soriano Hannah Georgia O. , Cabacungan Melbourne Klein B. , Millare Jeremiah C.
Published: February 2022

Kapok fiber (Ceiba pentandra) belongs to a group of natural fibers that are mainly composed of cellulose, lignin, pectin, and small traces of inorganic compounds. These fibers are lightweight with hollow tubular structure that is easy to process and abundant in nature. Currently, kapok fibers are used in industry as filling material for beddings, upholstery, soft toys, and nonwoven materials. However, kapok fiber has also a potential application in the adsorptive removal of heavy metal ions and dyes from aqueous systems. This study aims to provide a comprehensive review about the recent developments on kapok fiber composites including its chemical properties, wettability, and surface morphology. Effective and innovative kapok fiber composites are analyzed with the help of characterization tools such as scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, thermogravimetric analysis, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, and Brunauer-Emmett-Teller analysis. Different pre-treatment methods such as alkali and acid pre-treatment, oxidation pre-treatment, and Fenton reaction are discussed. These techniques are applied to enhance the hydrophilicity and to generate rougher fiber surfaces. Moreover, surface modification and synthesis of kapok fiber-based composites and its environmental applications are examined. There are various methods in the fabrication of kapok fiber composites that include chemical modification and polymerization. These procedures allow the kapok fiber composites to have higher adsorption capacities for selective heavy metal and dye removal.
Impact of Diet Consistency on the Mandibular Morphology: A Systematic Review of Studies on Rat Models
Authors: Karamani Ioanna I. , Tsolakis Ioannis A. , Makrygiannakis Miltiadis A. , Georgaki Maria , Tsolakis Apostolos I.
Published: February 2022

Apart from genetics, environmental factors, such as food consistency, may affect craniofacial morphology and development. The present systematic review aims to systematically investigate and appraise the available evidence regarding the effect of diet consistency on the anatomical structures of the basal bone of the rat mandible. The search was performed without restrictions in five databases (PubMed, Scopus, Web of Science, ProQuest Dissertations and Theses Global, including grey literature) and hand searching through January 2022. A total of 14,904 references were initially identified, and 16 articles were finally included in the systematic review. Rats that consumed hard diets were found to exhibit an increase inbigonial width, corpus height, condylar depth, condylar base inclination, condylar process inclination, mandibular plane inclination, height and length of angular process, mandibular body height, depth of antegonial notch, growth rate in the gonial angle, angular process convexity and height of condylar process. It was also noted that mandibular depth, mandibular height, ramus angle and angle between the angular process and mandibular plane were decreased in rats that were fed with a hard diet. On the other hand, there were conflicting results about the growth of mandibular length and width, corpus length, mandibular body length, ramus height, condylar length and width, gonial angle and height of coronoid process. From the abovementioned results, it can be concluded that food consistency may affect the morphology of anatomical structures and the overall growth and development of rat mandibles in various ways.
The Role of Seminal Oxidative Stress Scavenging System in the Pathogenesis of Sperm DNA Damage in Men Exposed and Not Exposed to Genital Heat Stress
Authors: Fraczek Monika , Lewandowska Angelika , Budzinska Marta , Kamieniczna Marzena , Wojnar Lukasz , Gill Kamil , Piasecka Malgorzata , Kups Michal , Havrylyuk Anna , Chopyak Valentina , Nakonechnyy Jozef , Nakonechnyy Andrij , Kurpisz Maciej
Published: February 2022

Responding to the need for the verification of some experimental animal studies showing the involvement of oxidative stress in germ cell damage in the heat-induced testis, we investigated the possibility of a direct relationship between seminal oxidative stress markers (total antioxidant capacity, catalase activity, superoxide dismutase activity, and malondialdehyde concentration) and ejaculated sperm chromatin/DNA integrity (DNA fragmentation and chromatin condensation abnormalities) in distinct groups of men exposed and not exposed to prolonged scrotal hyperthermia. A statistical increase in the proportion of sperm with DNA fragmentation was observed in all the studied subgroups compared to the fertile men. In turn, the groups subjected to heat stress as professional drivers or infertile men with varicocele presented greater disturbances in the oxidative stress scavenging system than men not exposed to genital heat stress. Based on the comparative analysis of the studied parameters, we can conclude that alterations in the seminal oxidative stress scavenging system are directly engaged in the pathogenesis of ejaculated sperm DNA damage regardless of the intensity of the impact of thermal insult. To the best of our knowledge, this study, for the first time, revealed the co-existence of oxidative stress and sperm DNA damage in the semen of professional drivers.
Semantic–Structural Graph Convolutional Networks for Whole-Body Human Pose Estimation
Authors: Li Weiwei , Du Rong , Chen Shudong
Published: February 2022

Existing whole-body human pose estimation methods mostly segment the parts of the body’s hands and feet for specific processing, which not only splits the overall semantics of the body, but also increases the amount of calculation and the complexity of the model. To address these drawbacks, we designed a novel semantic–structural graph convolutional network (SSGCN) for whole-body human pose estimation tasks, which leverages the whole-body graph structure to analyze the semantics of the whole-body keypoints through a graph convolutional network and improves the accuracy of pose estimation. Firstly, we introduced a novel heat-map-based keypoint embedding, which encodes the position information and feature information of the keypoints of the human body. Secondly, we propose a novel semantic–structural graph convolutional network consisting of several sets of cascaded structure-based graph layers and data-dependent whole-body non-local layers. Specifically, the proposed method extracts groups of keypoints and constructs a high-level abstract body graph to process the high-level semantic information of the whole-body keypoints. The experimental results showed that our method achieved very promising results on the challenging COCO whole-body dataset.
The Impact of Student Characteristics for Working with AR Technologies in Higher Education—Findings from an Exploratory Study with Microsoft HoloLens
Authors: Wyss Corinne , Degonda Adrian , Bührer Wolfgang , Furrer Florian
Published: February 2022

Augmented reality (AR) is being used in various fields and increasingly being used in the educational sector. Although there is still little research available on the use of this technology in education, the research literature indicates its potential and effectiveness. However, information about students’ characteristics and individual learning preconditions in relation to learning with AR technologies is still scarce. In this study, 18 student teachers for science and technology for lower secondary schools of a German-speaking university of teacher education were asked about different aspects related to motivation, interest, attitude, and self-efficacy before (questionnaire) and after (interviews) participating in an AR-supported teaching unit with Microsoft HoloLens. The results show that the students have a very positive attitude towards AR technologies and were highly motivated to work with this technology. Attitudes towards AR technologies, as well as towards new technologies in general, were found to be related to motivational factors and aspects of self-efficacy in the context of the AR teaching unit. As these aspects can have an impact on learning outcomes, it is of utmost importance to understand and recognize them. Educators and researchers should therefore pay more attention to these issues in the future.
Differential Selection on Caste-Associated Genes in a Subterranean Termite
Authors: Radford Julianne M. , Chen David , Chernyshova Anna M. , Taylor Cambrie , Guoth Alex W. , Wu Tian , Hill Kathleen A. , Thompson Graham J.
Published: February 2022

Analyzing the information-rich content of RNA can help uncover genetic events associated with social insect castes or other social polymorphisms. Here, we exploit a series of cDNA libraries previously derived from whole-body tissue of different castes as well as from three behaviourally distinct populations of the Eastern subterranean termite Reticulitermes flavipes. We found that the number (~0.5 M) of single nucleotide variants (SNVs) was roughly equal between nymph, worker and soldier caste libraries, but dN/dS (ratio of nonsynonymous to synonymous substitutions) analysis suggested that some of these variants confer a caste-specific advantage. Specifically, the dN/dS ratio was high (~4.3) for genes expressed in the defensively specialized soldier caste, relative to genes expressed by other castes (~1.7–1.8) and regardless of the North American population (Toronto, Raleigh, Boston) from which the castes were sampled. The populations, meanwhile, did show a large difference in SNV count but not in the manner expected from known demographic and behavioural differences; the highly invasive unicolonial population from Toronto was not the least diverse and did not show any other unique substitution patterns, suggesting any past bottleneck associated with invasion or with current unicoloniality has become obscured at the RNA level. Our study raises two important hypotheses relevant to termite sociobiology. First, the positive selection (dN/dS > 1) inferred for soldier-biased genes is presumably indirect and of the type mediated through kin selection, and second, the behavioural changes that accompany some social insect urban invasions (i.e., ‘unicoloniality’) may be detached from the loss-of-diversity expected from invasion bottlenecks.
Effect of Dietary Meat Content on Weight Gain, Mortality, and Pre-Pupal Rate in Black Soldier Fly (Hermetia illucens) Larvae
Authors: Kawasaki Kiyonori , Ohkawa Mami , Zhao Junliang , Yano Kiminobu
Published: February 2022

This study aimed to determine the protein content and the ratio of meat that can be added to the diet of black soldier fly (BSF) larvae for the sustainable recycling of food waste using insects. We conducted experiments feeding refined diets to BSF larvae with adjusted protein content and diets with minced pork, and analyzed the correlations between dietary nutrients and larval weight gain, mortality, and pre-pupal rate. The nutrient that was positively correlated with increased larval body weight and pre-pupal rate in both experiments was nitrogen-free extract (NFE). Diets with high concentrations of minced pork showed higher mortality of BSF larvae and were negatively correlated with dietary NFE content. It has been suggested that BSF larvae have reduced body weight and survival due to excessive protein and fat in their diet. Depending on the raw material and ratio of food waste, it may be necessary to adjust dietary protein and fat contents before treatment. The results of this study suggest that the NFE content of a larval diet influences the larval weight gain, mortality, and pre-pupal rate of BSF larvae in a great extent, a finding that has not been reported by previous studies.
Sex Differences in the Evaluation of Congestion Markers in Patients with Acute Heart Failure
Authors: Scicchitano Pietro , Paolillo Claudio , De Palo Micaela , Potenza Angela , Abruzzese Silvia , Basile Marco , Cannito Antonia , Tangorra Maria , Guida Piero , Caldarola Pasquale , Ciccone Marco Matteo , Massari Francesco
Published: February 2022

The impact of sex on the assessment of congestion in acute heart failure (AHF) is still a matter of debate. The objective of this analysis was to evaluate sex differences in the evaluation of congestion at admission in patients hospitalized for AHF. We consecutively enrolled 494 AHF patients (252 female). Clinical congestion assessment, B-type natriuretic peptide levels analysis, blood urea nitrogen to creatinine ratio (BUN/Cr), plasma volume status estimate (by means of Duarte or Kaplam-Hakim PVS), and hydration status evaluation through bioimpedance analysis were performed. There was no difference in medications between men and women. Women were older (79 ± 9 yrs vs. 77 ± 10 yrs, p = 0.005), and had higher left ventricular ejection fraction (45 ± 11% vs. 38 ± 11%, p < 0.001), and lower creatinine clearance (42 ± 25 mL/min vs. 47 ± 26 mL/min, p = 0.04). The prevalence of peripheral oedema, orthopnoea, and jugular venous distention were not significantly different between women and men. BUN/Cr (27 ± 9 vs. 23 ± 13, p = 0.04) and plasma volume were higher in women than men (Duarte PVS: 6.0 ± 1.5 dL/g vs. 5.1 ± 1.5 dL/g, p < 0.001; Kaplam–Hakim PVS: 7.9 ± 13% vs. −7.3 ± 12%, p < 0.001). At multivariate logistic regression analysis, female sex was independently associated with BUN/Cr and PVS. Female sex was independently associated with subclinical biomarkers of congestion such as BUN/Cr and PVS in patients with AHF. A sex-guided approach to the correct evaluation of patients with AHF might become the cornerstone for the correct management of these patients.
Inter-Eye Comparison of the Ocular Surface of Glaucoma Patients Receiving Surgical and Medical Treatments
Authors: Romano Dario , De Ruvo Valentino , Fogagnolo Paolo , Farci Roberta , Rossetti Luca Mario
Published: February 2022

Background: Ocular surface frequently affects glaucoma patients. In this paper we aimed at evaluating the impact of glaucoma surgery on the ocular surface of patients who received unilateral trabeculectomy. Methods: 26 consecutive patients successfully treated with trabeculectomy on one eye (Trab Eye) and under control with topical treatments on the fellow eye (Med Eye) were included in this observational study. They received IDEEL and OSDI questionnaires, Tear Film Osmolarity (TFO), grading of conjunctival hyperemia, fluorescein tear break-up time (tBUT), grading of corneal staining and Schirmer test. Results: IDEEL and OSDI scores were 48 ± 38 and 11 ± 12, respectively, with moderate correlation (r = 0.50, p = 0.03). Compared with Med eyes, Trab Eyes had higher tBUT (6.5 ± 3.5 vs. 5.1 ± 2.7 s, p = 0.004), lower conjunctival hyperemia (0.8 ± 0.9 and 1.7 ± 1.1 respectively, p < 0.001) and lower corneal staining (0.3 ± 0.5 and 0.6 ± 0.5, respectively, p = 0.03). Correlation between corneal staining and conjunctival hyperemia was 0.55 in Trab Eyes (p = 0.01) and 0.44 in Med Eyes (p > 0.05). Patients with bilateral corneal staining had had threefold worse questionnaire scores (p < 0.05). The duration of treatment and the daily exposure to preservatives did not directly affect OS parameters in this cohort of patients. Conclusions: Patients receiving successful trabeculectomy showed better OS homeostasis (higher TBUT, lower grading of conjunctival hyperemia and corneal staining) than fellow medically treated eyes. Presence of corneal epithelial damage in both eyes is the factor more consistently affecting questionnaire scores.
Is Hypertension Associated with Worse Renal Functional Outcomes after Minimally Invasive Partial Nephrectomy? Results from a Multi-Institutional Cohort
Authors: Flammia Rocco Simone , Anceschi Umberto , Tufano Antonio , Tuderti Gabriele , Ferriero Maria Consiglia , Brassetti Aldo , Mari Andrea , Di Maida Fabrizio , Minervini Andrea , Derweesh Ithaar H. , Capitanio Umberto , Larcher Alessandro , Montorsi Francesco , Eun Daniel D. , Lee Jennifer , Luciani Lorenzo G. , Cai Tommaso , Malossini Gianni , Veccia Alessandro , Autorino Riccardo , Fiori Cristian , Porpiglia Francesco , Gallucci Michele , Leonardo Costantino , Simone Giuseppe
Published: February 2022

Background: Hypertension (HTN) is a global public health issue. There are limited data regarding the effects of HTN in patients undergoing partial nephrectomy (PN) for renal tumors. To address this void, we tested the association between HTN and renal function after minimally invasive PN (MIPN). Methods: Using a multi-institutional database (2007–2017), we identified patients aged ≥ 18 years with a diagnosis of cT1 renal tumors treated with MIPN. Kaplan–Meier plots and Cox regression models addressed newly-onset CKD stage ≥ 3b or higher (sCKD). All analyses were repeated after 1:1 propensity score matching (PSM). Results: Overall, 2144 patients were identified. Of those, 35% (n = 759) were yes-HTN. Yes-HTN patients were older, more frequently male and more often presented with diabetes. Yes-HTN patients harbored higher RENAL nephrometry scores and higher cT stages than no-HTN patients. Conversely, yes-HTN patients exhibited lower preoperative eGFRs. In the overall cohort, five-year sCKD-free survival was 86% vs. 94% for yes-HTN vs. no-HTN, which translated into a multivariable HR of 1.67 (95% CI: 1.06–2.63, p = 0.026). After 1:1 PSM, virtually the same results were observed (HR 1.86, 95% CI: 1.07–3.23, p = 0.027). Conclusions: Yes-HTN patients exhibited worse renal function after MIPN when compared to their no-HTN counterparts. However, these observations need to be further tested in a prospective cohort study.
The Role of Vasospasm and Microcirculatory Dysfunction in Fluoropyrimidine-Induced Ischemic Heart Disease
Authors: Fabin Natalia , Bergami Maria , Cenko Edina , Bugiardini Raffaele , Manfrini Olivia
Published: February 2022

Cardiovascular diseases and cancer are the leading cause of morbidity and mortality globally. Cardiotoxicity from chemotherapeutic agents results in substantial morbidity and mortality in cancer survivors and patients with active cancer. Cardiotoxicity induced by 5-fluorouracil (5-FU) has been well established, yet its incidence, mechanisms, and manifestation remain poorly defined. Ischemia secondary to coronary artery vasospasm is thought to be the most frequent cardiotoxic effect of 5-FU. The available evidence of 5-FU-induced epicardial coronary artery spasm and coronary microvascular dysfunction suggests that endothelial dysfunction or primary vascular smooth muscle dysfunction (an endothelial-independent mechanism) are the possible contributing factors to this form of cardiotoxicity. In patients with 5-FU-related coronary artery vasospasm, termination of chemotherapy and administration of nitrates or calcium channel blockers may improve ischemic symptoms. However, there are variable results after administration of nitrates or calcium channel blockers in patients treated with 5-FU presumed to have myocardial ischemia, suggesting mechanisms other than impaired vasodilatory response. Clinicians should investigate whether chest pain and ECG changes can reasonably be attributed to 5-FU-induced cardiotoxicity. More prospective data and clinical randomized trials are required to understand and mitigate potentially adverse outcomes from 5-FU-induced cardiotoxicity.
Metal/Carbon-Fiber Hybrid Composites—Damage Evolution and Monitoring of Isothermal Fatigue at Low and Elevated Temperatures
Authors: Khatri Bilal , Rehra Jan , Schmeer Sebastian , Breuer Ulf , Balle Frank
Published: February 2022

Carbon-fiber-reinforced polymers (CFRPs) are the standard lightweight composite material for structural applications in aviation. The addition of metallic fibers to CFRPs to form metal/carbon-fiber hybrid composites (MCFRPs) has been shown to improve the elastic and plastic properties and to enable a non-destructive method for structural health monitoring over the material’s service life. In this paper, the results from the fatigue experiments on these hybrid composites at −55, 25 and 120 °C are discussed. Multidirectional CFRP and MCFRP laminates, fabricated using the autoclave method, were tested and compared under different fatigue loading conditions, while being simultaneously monitored for temperature and electrical resistance. Magnetic phase measurements were additionally carried out for the chosen metastable austenitic steel fibers in the MCFRPs. The results show that the improved ductility of the hybrid composite due to the presence of the steel fibers leads to better performance under fatigue loads and a less-brittle failure behavior. Based on the chemical composition of the metastable austenitic steel fibers, a temperature and plastic deformation-dependent phase transformation was observed, which could potentially lead to a method for non-destructive structural health monitoring of the hybrid composite over its service life.
Natural Frequencies Calculation of Composite Annular Circular Plates with Variable Thickness Using the Spline Method
Authors: Javed Saira
Published: February 2022

The present study adds to the knowledge of the free vibration of antisymmetric angle-ply annular circular plates with variable thickness for simply supported boundary conditions. The differential equations in terms of displacement and rotational functions are approximated using cubic spline approximation. A generalized eigenvalue problem is obtained and solved numerically for an eigenfrequency parameter and an associated eigenvector of spline coefficients. The vibration of the annular circular plates is examined for circumferential node number, radii ratio, different thickness variations, number of layers, stacking sequences and lamination materials.
Principal Component Analysis versus Subject’s Residual Profile Analysis for Neuroinflammation Investigation in Parkinson Patients: A PET Brain Imaging Study
Authors: Mabrouk Rostom
Published: February 2022

Dysfunction of neurons in the central nervous system is the primary pathological feature of Parkinson’s disease (PD). Despite different triggering, emerging evidence indicates that neuroinflammation revealed through microglia activation is critical for PD. Moreover, recent investigations sought a potential relationship between Lrrk2 genetic mutation and microglia activation. In this paper, neuroinflammation in sporadic PD, Lrrk2-PD and unaffected Lrrk2 mutation carriers were investigated. The principal component analysis (PCA) and the subject’s residual profile (SRP) techniques were performed on multiple groups and regions of interest in 22 brain-regions. The 11C-PBR28 binding profiles were compared in four genotypes depending on groups, i.e., HC, sPD, Lrrk2-PD and UC, using the PCA and SPR scores. The genotype effect was found as a principal feature of group-dependent 11C-PBR28 binding, and preliminary evidence of a MAB-Lrrk2 mutation interaction in manifest Parkinson’s and subjects at risk was found.
Scanning Hyperspectral Imaging for In Situ Biogeochemical Analysis of Lake Sediment Cores: Review of Recent Developments
Authors: Zander Paul D. , Wienhues Giulia , Grosjean Martin
Published: February 2022

Hyperspectral imaging (HSI) in situ core scanning has emerged as a valuable and novel tool for rapid and non-destructive biogeochemical analysis of lake sediment cores. Variations in sediment composition can be assessed directly from fresh sediment surfaces at ultra-high-resolution (40–300 μm measurement resolution) based on spectral profiles of light reflected from sediments in visible, near infrared, and short-wave infrared wavelengths (400–2500 nm). Here, we review recent methodological developments in this new and growing field of research, as well as applications of this technique for paleoclimate and paleoenvironmental studies. Hyperspectral imaging of sediment cores has been demonstrated to effectively track variations in sedimentary pigments, organic matter, grain size, minerogenic components, and other sedimentary features. These biogeochemical variables record information about past climatic conditions, paleoproductivity, past hypolimnetic anoxia, aeolian input, volcanic eruptions, earthquake and flood frequencies, and other variables of environmental relevance. HSI has been applied to study seasonal and inter-annual environmental variability as recorded in individual varves (annually laminated sediments) or to study sedimentary records covering long glacial–interglacial time-scales (>10,000 years).
Genetic Variability and Aggressiveness of Tilletia indica Isolates Causing Karnal Bunt in Wheat
Authors: Aasma  , Asad Shahzad , Fayyaz Muhammad , Majeed Khawar , Rehman Aziz ur , Ali Sajid , Liu Jindong , Rasheed Awais , Wang Yamei
Published: February 2022

Karnal bunt caused by Tilletia indica is a quarantine disease of wheat causing huge economic losses due to the ban on the import of bunted grains. This study was designed to characterize pathogenicity, aggressiveness and genetic diversity of 68 Tilletia indica isolates collected from different geographic regions of Pakistan. Forty-six isolates were tested for their pathogenicity on eight wheat varieties, out of which three were non-aggressive. The coefficient of infection (CI) ranged from 15.73% (PB-25) to 10% (PB-68, PB-60, and PB-43). The isolates collected from central Punjab showed higher infestation compared to other isolates. Among the wheat varieties used for the aggressiveness study, WL-711 showed susceptible reaction with 10.88% CI, while NIFA-Barsat, HD-29, Janbaz, Bakhtawar-92, Tatara, and AARI 2011 showed resistance to the highly resistant response. These isolates were amplified using 31 random amplified polymorphic DNA (RAPD) markers and 32 inter-simple sequence repeat (ISSR) markers for diversity analysis. The principal component analysis (PCA) and analysis of molecular variance (AMOVA) showed greater divergence among isolates collected from Punjab and Khyber Pakhtunkhwa (KPK), with a moderate level of admixture. The isolates from Faisalabad (Punjab) were more aggressive compared to isolates from KPK and were clearly separated based on PCA, indicating the significant genetic distance in the populations. Our findings will assist breeders and pathologists in better understanding the pathogenic variability in Tilletia indica and in subsequent disease management.
The Spectrum of Invasive Fungal Sinusitis in COVID-19 Patients: Experience from a Tertiary Care Referral Center in Northern India
Authors: Baghel Surendra Singh , Keshri Amit Kumar , Mishra Prabhakar , Marak Rungmei , Manogaran Ravi Sankar , Verma Pawan Kumar , Srivastava Arun Kumar , Kumar Raj , Mathialagan Arulalan , Bhuskute Govind , Dubey Abhishek Kumar , Dhiman Radha Krishan
Published: February 2022

This study aimed to determine the patient demographics, risk factors, which include comorbidities, medications used to treat COVID-19, and presenting symptoms and signs, and the management outcome of COVID-19-associated invasive fungal sinusitis. A retrospective, propensity score-matched, comparative study was conducted at a tertiary care center, involving 124 patients with invasive fungal sinusitis admitted between April 2021 and September 2021, suffering from or having a history of COVID-19 infection. Among the 124 patients, 87 were male, and 37 were female. A total of 72.6% of patients received steroids, while 73.4% received antibiotics, and 55.6% received oxygen during COVID-19 management. The most common comorbidities were diabetes mellitus (83.9%) and hypertension (30.6%). A total of 92.2% had mucor, 16.9% had aspergillus, 12.9% had both, and one patient had hyalohyphomycosis on fungal smear and culture. The comparative study showed the significant role of serum ferritin, glycemic control, steroid use, and duration in COVID-19-associated invasive fungal disease (p < 0.001). Headache and facial pain (68, 54.8%) were the most common symptoms. The most involved sinonasal site was the maxillary sinus (90, 72.6%). The overall survival rate at the three-month follow-up was 79.9%. COVID-19-related aggressive inflammatory response, uncontrolled glycemic level, and rampant use of steroids are the most important predisposing factors in developing COVID-19-associated invasive fungal sinusitis.