bluuebunny/crossref_metadata_2025 huggingface dataset:
GAMBARAN STRES KERJA PEGAWAI BAGIAN REKAM MEDIS RUMAH SAKIT BHAKTI WIRATAMTAMA SEMARANG
Authors: Sartika Dewi , Sugiharto Sugiharto
Published: September 2017

Faktor penyebab stres kerja adalah lingkungan kerja yang kurang nyaman, konsultasi yang tidak efektif dan konflik antara tuntutan keluarga dan tuntutan instansi. Penelitian kualitatif dilakukan untuk memperoleh gambaran stres kerja pegawai bagian rekam medis rumah sakit Bhakti Wiratamtama Semarang. Jenis penelitianini adalah penelitiankualitatif deskriptif menggunakan teknik snowball sampling. Informan utama berjumlah 15 pegawai dan 2 triangulasi. Hasil yang diperoleh, yaitu informan mengeluhkan mengalami stres kerja akibat rak fililng yang kurang rapi dan pengembalian dokumen rekam medis pasien yang terlambat dan lingkungan kerja yang kurang baik. Adanya pertemuan triwulan dapat mengurangi masalah yang dihadapi oleh pegawai. Saran bagi pegawai yaitu petugas filing melakukan pemilahan dokumen rekam medis antara lembar yang akan diabadikan dan dimusnahkan. Rumah sakit disarankan dalam pembersihan berkas pasien dan penataan ruang lebih ditata dengan rapi agar pegawai nyaman dalam mobilisasi dalam bekerja.

 

Factors that cause occupational stress are the inconvenient occupational environment, ineffective consultation

and conflict between family agency requirement. The purpose of this qualitative research is to get occupational

stress overview of medical record employee in Bhakti Wiratamtama Semarang Hospital. This research method

is qualitative research using snowball sampling. The informants consist of 15 key informants and 2

triangulation informants. The informans were complained about untidy shelves and late of medical record returning. Quarterly meeting to reduce this problem. Suggestion to employee was to sorting the medical record document, and to select wich one to be saved. Suggestion to hospital was to clean up patients documents and

tidy up the room so the employee can work with comfort.
PRAKTIK BIDAN DALAM PENGGUNAAN ALGORITMA MANAJEMEN TERPADU BAYI MUDA PADA KUNJUNGAN NEONATAL
Authors: Iraningsih Wahyu , Azinar Muhammad
Published: September 2017

Neonatal mortality in Indonesia declined slowly and tended to stagnant in number of 20/1000 live births. Proportion of neonatal mortality in Cilacap Regency at 2015 reached 69% of infant mortality. A quality neonatal care might decrease 34-62% mortality, but not all midwives used integrated management of neonatal and childhood illness (IMNCI) algorithm. This study aimed to figure out factors associated with midwives’ practice on using IMNCI algorithm in neonatal care. This study used cross sectional approach with purposive sampling until 60 respondents were collected. Data were analyzed with chi-square test. There were association between knowledge (p=0.000), attitude (p=0.023), availability of facilities (p=0.023), support from leader (p=0.017), length of work (p=0.011), and supervision (p=0.039) with midwives’ practice on using IMNCI algorithm. There were not assocation on age (p=0.124), employment status (p=0.124), extra work load (p=0.290), and reward (p=0.053). Factors associated with midwives’ practice on using IMNCI algorithm in neonatal care were knowledge, attitude, availability of facilities, support from leader, length of work, and supervision.
EVALUASI PELAKSANAAN PROGRAM TERAPI RUMATAN METADON PADA PECANDU NARKOBA JENIS PUTAW DI KOTA SEMARANG TAHUN 2016
Authors: Juniawati Juniawati , Indriyanti Dyah Rini
Published: September 2017

Methadone maintenance treatment (MMT) is aimed to reduce the harmful effects of injecting drug use. Since the first Puskesmas Poncol opened this program in 2010, there were 50 users of MMT. Currently users of MMT were 11 people, 2 people have died, no patient has recovered, and 36 others dropped out. Those did not recover, but dropped out with reasons and information that were not known by the officer. MMT is a strategic key of HIV/ AIDS prevention for injecting drug users. However, in the implementation Puskesmas Poncol was lost most of active MMT users. The research aimed to know the implementation of MMT which includes input, process, and output on heroin addict in Puskesmas Poncol. This research was qualitative research supported by quantitative data. Data collection used in-depth interviews, observation, and documentation. The result of the research showed that the service of MMT in Puskesmas Poncol was still many weaknesses, from input aspect (untrained human resources), process (methadone planning, organizing, and dose arrangement have not been well implemented), and output (services have not been evaluated).
KEEFEKTIFAN PETUGAS SURVEILANS KESEHATAN DEMAM BERDARAH DENGUE DALAM MENENTUKAN ANGKA BEBAS JENTIK
Authors: Resmi Lestariana Putri Citra , Laksono Budi
Published: September 2017

Health surveillance officers of dengue hemorrhagic fever (DHF) was one of DHF prevention and control programs Semarang City. The aim of this research was to know the effectiveness of DHF surveillance officers to determine larvae-free rate in Semarang City. This research was quantitative supported by qualitative study with descriptive survey design. Samples in this research were 57 DHF health surveillance officers determined by cluster sampling technique and 3 of them as informants to complete the quantitative data. Techniques of collecting data were interviews and documentation. Data analysis was done descriptively by determining the effectiveness formula and using Miles and Hubberman model for qualitative data. The study showed that DHF health surveillance officers in Semarang City has been effective. It showed from effectiveness result score of each component compared with the percentage table of program effectiveness that input aspect was effective (71%), process aspect was very effective (92%), and output aspect was effective (77%), even though there were still obstacles in each aspect.
Pengetahuan dan Sikap Terhadap Perilaku dalam Penanggulangan Malaria di Kelurahan Sukarami Kota Bengkulu
Authors: Triana Dessy , Rosana Elvira , Anggraini Rizkianti
Published: September 2017

Indonesia merupakan salah satu negara yang masih berisiko terhadap malaria dengan prevalensi sebesar 1,4% dan angka insiden sebesar 0,3% dengan angka Annual Parasite Incidence (API) tahun 2015 sebesar 0,85%. Provinsi Bengkulu memiliki angka prevalensi sebesar 1,5% dan angka insiden sebesar 5,7% dengan angka API sebesar 2,03% yang menduduki urutan ke-6 angka API terbesar di seluruh Indonesia. Tujuan penelitian ini adalah untuk mengetahui hubungan antara pengetahuan dan sikap terhadap perilaku dalam penanggulangan Malaria. Penelitian ini merupakan observasional analitik dengan rancangan penelitian cross sectional. Sampel dipilih secara acak. Analisis data dilakukan dengan uji regresi logistik. Hasil penelitian menunjukkan bahwa terdapat hubungan bermakna antara pengetahuan tentang malaria terhadap perilaku penanggulangan malaria (p value= 0,001; OR=4,237), tetapi tidak terdapat hubungan yang signifikan antara sikap tentang malaria terhadap perilaku penanggulangan malaria (p value = 0.392). Proporsi pengaruh variabel pengetahuan dan sikap terhadap variabel perilaku penanggulangan malaria sebesar 17,9%.

Indonesia has a risk of Malaria with the prevalence of 1.4%, incidence rate of 0.3%, and Annual Parasite Incidence (API) rate in 2015 of 0.85%. Bengkulu Province had prevalence rate of 1.5% and incidence rate of 5.7% with API rate of 2.03% which ranked 6th highest API ratein Indonesia. The purpose of this study was to determine association between knowledge and attitude with behavior in Malaria prevention. This research was analytical observational study with cross sectional design. Sample was randomly selected. Data were analyzedwith logistic regression test. The results showed that there was significant association between Malaria knowledge with Malaria prevention behavior (p value = 0,001; OR = 4,237), but there was no significant association between Malaria attitude with Malaria prevention behavior (p value = 0.392). The proportion of knowledge and attitude variables’ influence to Malaria prevention behavior variable was 17,9%.
KAJIAN IMPLEMENTASI KEBIJAKAN RUANG LAKTASI DI SEKTOR PEMERINTAH DAN SWASTA
Authors: Wijaya Prawiti Sugeng , Soesanto Soesanto Soesanto
Published: September 2017

ABSTRAK

Masalah pemberian ASI menjadi salah satu issue kesehatan di dunia. Rendahnya konsumsi ASI bagi bayi, salah satu penyebabnya adalah faktor lingkungan yang belum kondusif mendukung ibu untuk tetap dapat memberikan ASI Eksklusif. Implementasi Kebijakan Pemerintah tidak selalu berjalan sesuai dengan harapan, sehingga perlu dilakukan studi implementasi baik di sector pemerintah maupun swasta.Tujuan penelitian yaitu untuk menganalisis implementasi kebijakan ruang laktasi yang diselenggarakan pemerintah dan swasta di kabupaten Purbalingga.

Pendekatan yang digunakan adalah pendekatan kualitatif dengan desain studi kasus. Sasaran penelitian adalah pemimpin perusahaan, penanggungjawab ruang laktasi dan pengguna ruang laktasi di PT Royal Korindah dan KPP Pratama kabupaten Purbalingga dan informan tambahan dari bagian promosi kesehatan Dinas Kesehatan kabupaten Purbalingga serta bagian HPTK Dinas Tenaga Kerja kabupaten Purbalingga. Pengambilan data dilakukan melalui wawancara mendalam dan observasi sistematis.

Hasil penelitian adalah implementasi kebijakan ruang laktasi yang diselenggarakan oleh sektor pemerintah dan swasta di kabupaten Purbalingga belum dilaksanakan sesuai dengan ketentuan yang ada.

 

ABSTRACT

The low consumption of breast milk for infants, one reason is the environment that is not conducive factor supporting the mother to remain exclusive breastfeeding. Implementation of Government policies do not always go according to expectations. Object of this study was to analyze the implementation of lactation space policy organized by the government and private sectors in Purbalingga district.

The approach used is qualitative approach with case study research design. Goal of research is the leader of the company, responsible for lactation space and user space lactation PT Royal Korindah and STO The Purbalingga and additional informants from the health promotion Purbalingga District Health Office and the Department of Labor HPTK part Purbalingga district. Data were collected through interviews and systematic observation.

Results of the research is the implementation of space policy lactation organized by public and private sectors in Purbalingga has not been implemented in accordance with existing regulations.
HUBUNGAN TEMAN SEBAYA, CITRA TUBUH DAN POLA KONSUMSI DENGAN STATUS GIZI REMAJA PUTRI
Authors: Normate Erni Susana , Nur Marselinus Laga , Toy Sarci Magdalena
Published: September 2017

Abstrak

Teman sebaya dan citra tubuh merupakan faktor yang dapat menyebabkan remaja putri merasa kelebihan berat badan dan merasa tidak puas terhadap bentuk tubuhnya. Remaja tersebut akan menerapkan perilaku makan yang tidak sehat yang akhirnya berdampak pada status gizi yang buruk. Tujuan penelitian ini adalah untuk menganalisis hubungan teman sebaya, citra tubuh dan pola konsumsi dengan status gizi remaja putri SMAN 1 Kupang. Metode penelitian yang digunakan adalah survei analitik dengan rancangan cross sectional study. Populasi penelitian adalah seluruh siswi yang ada di SMAN 1 Kupang dan sampel penelitian sebesar 88 responden yang diperoleh dengan teknik simple random sampling. Hasil penelitian menunjukkan bahwa variabel yang mempunyai hubungan dengan status gizi remaja putri yaitu teman sebaya sedangkan variabel yang tidak mempunyai hubungan yaitu citra tubuh dan pola konsumsi. Upaya peningkatkan pengetahuan remaja tentang diet yang benar sangat diperlukan agar tidak mudah terpengaruh oleh teman sebaya dan melakukan diet yang salah.

 

Abstract

Peers and body image are factors that caused adolescents to feel overweight and dissatisfied toward her body shape. Then adolescents will ted to eat unhealthy food that results in a low nutritional status. The aim of this study was to analyze the relationship of peer, body image, and consumption pattern with nutritional status of adolescents at SMAN 1 Kupang. Research method used was analytical survey with cross sectional study design. The population of this study was all students in SMAN 1 Kupang and sample of this study was 88 respondents selected by using simple random sampling technique. Peers   were significantly related to nutritional status of adolescents. While the variables that had no relationship were body image and consumption pattern. The efforts to increase knowledge about proper diet is necessary for adolescents so that they could not easily affected by peers and have wrong diet.
ID:4003 COP9 signalosome in regulating EGFR oncogenic signals
Authors: Lee Mong-Hong , Fang Lekun , Chen Bo , Choi Hyun-Ho , Shin Ji-Hyun , Phan Liem , Yeung Sai-Ching
Published: September 2017

The constitutive photomorphogenesis 9 signalosome (CSN) regulates the stability of tumor suppressor and oncogenic proteins via proteasome-mediated protein degradation. The detailed regulatory mechanisms of CSN subunit expression in tumorigenesis remain to be illustrated. We demonstrated several important biological functions of CSN6: p53 signal transduction,neddylation regulation of Cullin, F-box protein ubiquitination, and Myc stability regulation. Biochemical studies demonstrated that CSN6 is involved in cell cycle regulator stability function and various cancer signaling pathways, including epidermal growth factor receptor (EGFR) pathway. We show that CSN6 overexpression is frequently observed in human colorectal cancers, which leads to drug resistance in anti-EGFR treatment and is correlated with poor clinical survival. We present amechanism for the role of CSN6 in ERK signaling cascade and provide new insights into the functional role of CSN6 deregulation during tumorigenesis.
ID:4004 Stem cell drugs: the next generation of pharmaceutical products
Authors: Pham Phuc Van
Published: September 2017

Stem cells represent a new treatment option in medicine and pharmacy. Stem cells have been increasingly used for the treatment of many diseases. In fact, they have spurred a new age of medicine called regenerative medicine. In recent years, regenerative medicine has become a new revolution in disease treatment, especially with the use of stem cell drugs. Stem cell drugs refer to live stem cell based products that used as drugs for particular diseases. Unlike autologous stem cell transplantation, stem cell drugs are “off-the-shelf” products that are ready to be used without requirement of any further manipulation. This review aims to summarize some of the approved stem cell drugs, and discuss the revolution of regenerative medicine and personalized medicine. As well, the review will discuss how stem cell drugs have led to a new direction in stem cell therapy, providing a new platform for patient needs.
ID:3002 Notch-mediated control of memory T cells against cancer cells
Authors: Yasutomo Koji
Published: September 2017

T cells recognize an antigen presented by self-MHC, and the part of initially activated T cells differentiate toward memory T cells. T cells also recognize cancer cells leading to generation of memory T cells against cancer-derived antigens although the activity of T cells are frequently suppressed by various factors. The release from T cell inhibitory factors could allow T cells to respond to cancer cells. However, it remains unclear which molecules are required for long-term survival of memory T cells and generation of memory T cells against cancer cells. Notch functions as a regulator for fate decision, activation and survival of immune cells. We have demonstrated the roles of Notch in mature T cell differentiation and found that Notch signaling is essential for the maintenance of memory CD4 T cells. The inhibition of Notch disturbs the survival of memory CD4 T cells. The effect of Notch on T cell survival depended on glucose uptake through cell surface Glut1 expression. We revealed that Notch is crucial for the long-term survival of memory T cells against cancer cells and suppression of Notch signaling reduced the tumor antigen-specific killing of cancer cells. Those data demonstrate that Notch is pivotal for the maintenance of memory T cells against cancer cells and suggest that activation of Notch signaling might be advantageous to cancer immunotherapy.
ID:3005 Combination of vaccine-strain measles and mumps viruses enhances oncolytic activity against human solid malignancies: special focus on prostate cancer
Authors: Nguyen Toan Linh , Son Ho Anh , Zhang LiFeng , Cuong Bui Khac , Tong Hoang Van , Cuong Le Duy , Nhung Hoang Thi My , Yamamoto Naoki
Published: September 2017

Oncolytic viruses (OLVs) including measles and mumps viruses (MeV and MuV) have a potential to serve as a therapeutic option for cancers. We have previously shown that the combination of MeV and MuV synergistically kills various human haematological cancer cells. This study aims to investigate the anti-tumor activity of MeV, MuV and MeV-MuV combination (MM) against human solid malignancies in vitro and in vivo. The results showed that MeV, MuV and MM combination targeted and effectively killed various cancer cell lines of human solid malignancies but not normal cells. Notably, MM combination demonstrated a greater anti-tumor effect and prolonged survival in a human prostate cancer (PC3) xenograft tumour model compared to MeV and MuV. MeV, MuV and MM combination significantly induced the expression of immunogenic cell death (ICD) markers and enhanced spleen-infiltrating immune cells such as macrophages, natural killer and dendritic cells. Our study demonstrated that MM combination is a promising option for treatment of human solid malignancies and suggested that MM could induce immunogenic cell death of malignant cells and activate immunity against cancers.
ID3009 Gelatin-Poly (Ethylene Glycol) Methyl Ether-Functionalized Porous Nanosilica for Controlled Doxorubicin Delivery
Authors: Nguyen Hai Dai
Published: September 2017

Porous nanosilica (PNS) has been attracting a growing attention in fabrication of carriers for drug delivery system (DDS). However, unmodified PNS-based nanocarriers exhibit the initial burst release of encapsulated bioactive molecules, which may limit their potential clinical applications. In this report, the surface of PNS was conjugated with gelatin-poly (ethylene glycol) methyl ether (GEL-mPEG) to form a core-shell structure PNS-GEL-mPEG for doxorubicin (DOX) delivery. The conjugated PNS carriers were found to be spherical in shape with diameter range of approximately 55-85 nm as compared with their parentally PNS (55-67 nm). The PNS-GEL-mPEG nanoparticles showed their ability to effectively encapsulate DOX for controlled release. In detail, DOX was efficiently loaded into the PNS-GEL-mPEG to form DOX-loaded nanocarriers (DOX@PNS-GEL-mPEG) with high loading efficiency (79.7%). The release of DOX from DOX@PNS-GEL-mPEG was prolonged and controlled up to 96 h in phosphate buffered saline (PBS, pH 7.4, 37 oC) without any initial burst release. These results demonstrated that this PNS-GEL-mPEG can be a potential candidate for controlled DDS with high loading capacity in cancer therapy
ID3017 New strategy in development of cancer chemopreventive agent using Malaysia Plant
Authors: Seeni Azman
Published: September 2017

Cancer had become worldwide problem that efforts in prevention and treatment of cancer had rapidly grew in any research institute. Meanwhile, epidemiological studies have demonstrated a positive correlation between consumption of vegetables, fruits and beverages with reduced risk of cancer. It is estimated there are around 8,100 plant species in the Malaysia rain forests, with 10% of them reported to have some medicinal value. However, to date not much investigation has been done on chemopreventive activities on cancer. Therefore, we had studied the chemopreventive activity using various type of local plants such as Christia sp, Nephelium lappaceuem, Cocos nucifera and many more others which we going to present here. But, to combat with this miserable disease, we still need new strategies to overcome the problem and we also utilized the waste product from our tropical fruits and Paddy waste which might have beneficial effect towards cancer chemoprevention activities. We also going to present our strategy in developing mouthwash product to prevent oral cancer using bonsai plant, Streblus asper with demonstrable efficacy against defined molecular target on cancer cell line as well as in animal models. It is clearly shown that S. asper root extracts that have the anti-oxidant characteristic with analgesic properties via Inositol, not only kill oral-microbes but interestingly also inhibit the growth of osteosarcoma cells, tongue carcinoma cells and cervical cancer cells. The inhibition effect was through the induction of apoptosis signaling pathway. This mouthwash product also is non-toxic for the liver, kidney and skin fibroblast cells and no heavy metals were found. Currently, we now focused the use of the same product on cervical cancer and might develop it as a ladies hygiene product which act as chemopreventive agent. Futhermore S.asper also can suppressed the proliferative activity of the epithelium cells in Benign Prostate Hyperplasia animal model. Lets combat the cancer disease using new strategies.
ID3018 Direct reprogramming of mouse somatic cells into pluripotent stem sells by pig germinal vesicle oocytes extract
Authors: Nguyen Thuan Van
Published: September 2017

Genomic reprogramming factors in the cytoplasm of mature oocytes could be reprogrammed somatic cell cells to totipotency cells and full-term development (cloned animals). Since then, this technique has been considered an important toot not only for animal reproduction but also for regenerative medicine, concervation of endangered species, and for study of genes function and cell biology. Moreover, in order to produce nuclear transfer embryonic stem (ntES) cells using somatic cell nuclear transfer (SCNT), the SCNT technique requires donated fresh oocytes, which raises ethical problems for production in human cloned embryo. For this reason, the use of induced pluripotent stem (iPS) cells for genomic reprogramming and for regenerative medicine is currently a hot topic in this field. However, the use of iPS cells for human therapy by the technique of retroviruses used to insert the pluripotent genes into somatic cells could cause tumors in tissues grown from the host iPS cells. Recently, we found that genomic reprogramming factors in the cytoplasm of pig germinal vesicle (GV)-stage oocytes has been shown to improve the efficiency of producing cloned mouse offspring and could reprogram pig fibroblasts to stem-like cells. In this talk, we will discuss whether pig GV cytoplasmic extract could induce pluripotent stem cells from mouse fibroblast cells (interspecies reprogramming). We first established stem-like cells from mouse fibroblast cells treated with GV oocytes extracted (gviPS cells). We demonstrated that reactivation of Oct4 promoter in mouse Oct4-GFP fibroblast cells at day 10 after treated with pig GV oocyte cytoplasmic extract and the formation of colonies is observed at 3 weeks after treatment. In addition, mouse gviPS cells reprogrammed with pig GV cytoplasmic extract can in vitro re-differentiate into neuron-like cells. Interestingly, mouse gviPS cells injected into embryos of different mouse strain could be produced chimeric mice.
ID3019 Female germline stem Cells: A source for application in reproductive and regenerative medicine
Authors: Bui Thuy Hong
Published: September 2017

Studies suggest a renewable source of eggs and stir more controversy, especially about the origin of female germline stem cells (FGSCs). It should be elucidated whether or not neo-oogenesis continues in the ovaries of mammalian female during postnatal life. Therefore, the establishment of FGSCs is very important for many applications. Here, using adult pig ovary, we isolate, identify, characterize FGSCs to elucidate their origin, then examined the proliferation, growth and differentiation of them. These cells were heterogeneous, depending on both of c-kit expression and cell size, and also express stem cell and germ cell markers. Importantly, we show clearly that the cells with the characteristics of early primordial germ cells are present in the adult pig ovary. Once FGSCs were established, they could be expanded in vitro for months without loss of the identifying markers and proliferative potential. Under appropriate conditions, the FGSCs differentiated into primordial oocyte-like cells and grow close to full-sized oocytes. These may assist in therapeutic strategies in human with their potential to make new oocytes and support ovarian function and fertility. Our results support the theory that the ovary contains a small number of undifferentiated cells with stem cell characteristics. These might remain in the postnatal and adult ovary and under certain conditions could resume mitosis, enter meiosis and give rise to oocytes. Given the existence of these FGSCs in mammalian ovaries and the depletion in ovarian reserve during female reproductive aging, one can hypothesize that such “neo-oogenesis” was present in ancestral forms, is still present in insects, some fish and mollusks, but has been lost in land vertebrates through evolution. FGSCs cannot proliferate in the ovary normally because of inhibitory factors, but under appropriate conditions, they can undergo proliferation and differentiation, and provide a potential mechanism for the self-renewal of germline stem cells.
ID3020 Stem cell transplantation for incurable diseases in children
Authors: Nguyen Liem Thanh
Published: September 2017

Background: Stem cell transplantation has been widely performed to treat many different diseases in adults.  However, its indication is still limited in children. Since 2014, we have been doing stem cell transplantation for various incurable diseases in children. The aim of our report  is to evaluate safety and efficacy of stem cell transplantation for incurable diseases in children.

Results

From 2014-2017, stem cell transplantation has been performed for different conditions  including :

Cerebral palsy due to oxygen deprivation, cerebral palsy related to neonatal icterus , Cerebral palsy after meningitis/encephalitis , cerebral palsy after brain trauma ,

Brain damage due to drowning

Autism

Liver cirrhosis due to biliary atresia

Bowel and urinary dysfunction after meningocele repair

Brongchopulmonary dysplasia

Intestinal neural dysplasia

There were no death related to stem cell transplantation. No severe side effects occurred.  Early outcomes are promising.

 Conclusion : Stem cell transplantation is safe and effectiveness in treatment of different incurable diseases in children
ID:2022 Transoral Laser Microsurgery for Early Glottic Cancer
Authors: Tran Phan Chung Thuy , Nguyen Tuan Thanh
Published: September 2017

Background: For the treatment of early glottic cancer (T1/T2N0M0), different treatment options, such as transoral laser microsurgery, open surgical techniques, and primary radiotherapy, are under discussion. In this context, the aim of the present study was to describe oncologic results and complication rates of transoral laser microsurgery in treatment of early glottic cancer.

 

Methods: A prospective cohort study in Ho Chi Minh City Ear Nose Throat Hospital included early stages (T1/T2N0M0) glottic cancer patients scheduled to undergo transoral laser microsurgery from January 2016 until June 2017. Data on demographics, site and extent of disease, treatment, complication and early outcome were collected and analyzed.

 

Results: Twenty eight early stages glottic cancer cases were exclusively treated by TLM and included into this study, 27 (96.4%) patients were male and 1 (3.6%) were female. The mean age was 61.8 years (± 8 years). 92.9% tumors is T1 and 7.1% for T2. The presence of intraoperative complications was low, affecting only 3.6% of patients. Immediate postoperative complications occurred in 7.1%, whereas delayed complications affected 10.7% of patients, without any of them being fatal. The most frequent complication of TLM was granulation tissue formation. 30% of patients had normal voices and a further 48% had only mild or moderate voice change. At their last follow up, no patients assessed had any difficulty swallowing relating to their treatment for glottic cancer.

 

Conclusion: TLM has shown good results and low complication rate compared with traditional open surgery during intervention, in the immediate and delayed postoperative period and in the long-term with respect to radiotherapy, and thus shows utility as a primary treatment modality for early glottic cancer.
ID3024 Polymeric nanoparticles display bactericidal effect and selective fermentation for the treatment of acne vulgaris
Authors: Hsieh Ming-Fa
Published: September 2017

The use of antibiotics in the treatment of acne in specific group (pregnant women) of patients can lead to serious complications. We have previously demonstrated that the nanoparticles made of block copolymers of poly (ethylene glycol) and poly(e-caprolactone) can inhibit the growth of Propionibacterium acnes (P. acnes), a bacterium highly associated with the progress of acne vulgaris in the human skin [Polymers 2016; 8, 321]. To reduce the amount of antibiotics used in the treatment of skin acne, we have further demonstrated that a bacterium in the human skin microbiome can utilize PEG-based polymers to produce various short-chain fatty acids (SCFAs) which suppressed the growth of P. acnes. PEG-based polymers were chosen as selective fermentation initiators which specifically induced the fermentation of the skin commensal bacterium but not P. acnes. Interestingly, PEG-based polymers can efficiently suppress the growth of P. acnes. An acne ex vivo explant was established by using acne biopsies collected from patients with acne vulgaris at the early and middle stages. The levels of pro-inflammatory interleukin (IL)-8 cytokine in early- and middle-staged acnes were significantly higher than those in healthy skins. Incubation of acne ex vivo explants with sucrose remarkably reduced the level of IL-8 and the number of P. acnes. Results from mouse studies revealed that PEG-based polymer functions as antibiotic adjuvants which can considerably reduce the effective doses of clindamycin, a clinically-used acne antibiotic
ID3025 Allogenic mesenchymal stem cell transplantation for chronic obstructive pulmonary disease
Authors: Le Phuong Thi-Bich
Published: September 2017

Will be updated soon.
ID3026 Current use and clinical studies of stem cells therapy for osteoarthritis in Viet Nam
Authors: Tran Tung Dang-Xuan
Published: September 2017

Mesenchymal Stem Cell Therapy (MSC) is one of the newest and most advanced therapies in Regenerative and Integrative Medicine for a variety of medical problems that are not easily treated with traditional conventional allopathic medical approaches. There is an abundance of clinical experience by numerous physicians, patients and researchers proving consistently that mesenchymal stem cell-MSC therapy can be extremely helpful in Osteoarthritis and Rheumatoid Arthritis. In Viet Nam we have several researches in stem cells clinical application in OA
ID:2024 Synthesis and Characterization of PLGA-PEG Thymoquinone Nanoparticle and its Cytotoxicity Effects in Tamoxifen-resistant Breast Cancer Cells
Authors: Ahmad Rozaina , Kaus Noor Haida Mohd , Hamid Shahrul
Published: September 2017

Background: Drug resistance has been a continuous challenge in cancer treatment. The use of nanotechnology in the development of new cancer drugs has potential. One of the extensively studied compound is thymoquinone (TQ) and this work aims to compare two types of TQ-nanoformulation and its cytotoxicity towards resistant cancer cells.

Methods: TQ-nanoparticles were prepared and optimized by using 2 different formulation with different drug to PLGA-PEG ratio (1:20 and 1:7) and different PLGA-PEG to pluronic F68 ratio (10:1 and 2:1). The morphology and size were determined using TEM and DLS. Characterization of particles were done using UV-VIS, ATR-IR, entrapment efficiency and drug release. The effects of drug, polymer and surfactants were compared between the two formulations. Cytotoxicity assay was performed using MTS assay.

Results: TEM finding showed 96% of particles produced with 1:7 drug to PLGA-PEG were less than 90 nm in size and spherical in shape. This was confirmed with DLS which showed smaller particle size than those formed with 1:20 drug to PLGA-PEG ration. Further analysis showed zeta potential was negatively charge which could facilitate cellular uptake as reported previously. In addition, PDI value was less than 0.1 in both formulations indicating monodispersed and less broad in size distribution. The absorption peak of PLGA-PEG-TQ-Nps were at 255 nm. The 1:7 drug to polymer formulation was selected for further analysis where the entrapment efficiency was 79.9% and in vitro drug release showed a maximum release of TQ at 64% respectively. Cytotoxicity result showed IC50 of TQ-nanoparticle at 20.05 µM and free TQ was 8.25 µM.

Conclusion: This study showed that nanoparticle synthesized with 1:7 drug to PLGA-PEG ratio and 2:1 PLGA-PEG to pluronic F68 were less than 100 nm and had spherical shape as confirmed with DLS. This could facilitate its transportation and absorption to reach its target. There was conserved TQ stability as exhibited by slow release of this volatile oil. Cytotoxicity effect was noted when resistant breast cancer cells were treated with these particles.
ID2011 Potential strategies to improve Temozolomide/ radiation therapy of malignant gliomas by targeting glycolytic energy metabolism
Authors: Thakur Kalyani
Published: September 2017

The current standard treatment of Malignant Gliomas includes surgery, followed by Temozolomide (TMZ) - Radiotherapy. It leads to increased survival as compared to radiotherapy alone. However, haematological toxicities are also increased by the combination treatments. Therefore, it is important to carry out further preclinical studies, to develop more effective treatment for these tumors. 2-Deoxy-D-Glucose (2-DG), an inhibitor of glycolytic energy metabolism, has been shown earlier to differentially inhibit repair processes, growth and survival of cancer cells. It has been tolerated very well in combination with radiotherapy in clinical trials on Malignant Gliomas. In this study, we investigated the effects of combination of clinically relevant concentrations of 2-DG (0.5mM and 1mM) and TMZ (2µM and 5µM) ± Radiation (1Gy) on cell proliferation, total cellular damage (TCD) and colony formation in an established Glioblastoma cell line (U251MG), and primary cultures derived from malignant glioma tumor pieces. The monolayer cultures were grown on cover slips and stained with Acridine Orange (0.002%) after drug treatments and irradiation, for the study of cellular damage and death. Exponentially growing cells were exposed to drugs and Gamma irradiation. Drugs were removed 4 hrs after irradiation and cultures were processed for different assays of cell death and damage. Our results showed that combination of 2-DG with TMZ±Radiation significantly inhibited the cell proliferation up to 6 days. The TCD was significantly increased by the combination of drugs in primary as well as in established cell line. Cell proliferation as measured by MTT assay showed that drugs ± radiation significantly reduced proliferation response. Cell survival inhibition after combination of TMZ+2-DG+Gamma (2µM+0.5mM+1Gy) showed more than additive effects. These results suggest that targeting glycolytic energy metabolism by agents such as 2-DG can enhance the efficacy of TMZ+Radiation Therapy for Malignant Gliomas, without increasing toxic side effects
ID2015 Preparation of graphene oxide/oligopeptides composite for promoting mesenchymal stem cell proliferation
Authors: Puah Perng Yang
Published: September 2017

Human mesenchymal stem cells (hMSCs) treatments are being tested clinically for a range of disorders. However, ex vivo expansion of hMSCs is still depending on human or animal source extracellular matrix (ECM) molecules. These ECM molecules are expensive to manufacture, have limited scalability and high batch-to-batch variability. Surface modification techniques have been instrumental in the development of scaffolds that promote cell-surface interactions. In this study, the surface of graphene oxide (GO) was modified in order to promote the attachment and proliferation of mesenchymal stem cells (hMSCs). YIGSR, WYQNMIR, IKVAV and QHREDGS peptide sequences derived from ECM adhesion peptides were combined alternatively to form oligopeptides and each oligopeptide was attached to GO through π-π and electrostatic interactions. The presence of peptide bond in the GO/peptide composites were confirmed by using modified Lowry method. The GO/Peptide-IKVAV composite showed increase proliferation rate and better attachment of hMSCs at day 1 as compared to GO substrate. In addition, the GO/Peptide-IKVAV composite proved to be a suitable environment for the time-dependent viability of hMSCs. The data obtained here collectively demonstrates that the GO/Peptide-IKVAV composite is a potential substrate for the adhesion and proliferation of hMSCs
ID2017 Cytotoxic activities of Philippine marine sponges against colon cancer cells
Authors: Uy Mylene
Published: September 2017

Although the diversity of life in the terrestrial environment is exceptional, the greatest biodiversity is in the marine environment. Among the marine organisms, the sponges (Porifera) are the most prolific sources of bioactive secondary metabolites. The Philippines, with its long coastal lines, has drawn on its marine capital only to a small extent. Only a few marine organisms (ascidians, sponges, other marine invertebrates and their associated microorganisms) collected from various parts of the Philippines have been documented and investigated in terms of their potential as source of bioactive secondary metabolites, particularly anticancer compounds.  Thirty-seven sponges from the coasts off Mindanao, Philippines were collected, identifies and extracted to give a total of seventy-four polar and nonpolar extracts. The corresponding sponge extracts were screened for the sponge extracts were investigated for cytotoxicity towards colon cell lines (HCT116) using the the tetrazolium dye  (3-(4,5-Dimethylthiazol-2-yl)- 2,5- diphenyltetrazolium bromide) [MTTmethod. The results indicated fifteen (15) sponge extracts to be active at 100- microgram/mL  concentration among which seven (7) were still active at 10 microgram/mL while two (2) still exhibited activity at 1 microgram/mL. Further investigation of the active sponge extracts is currently in progress
ID2018 Radioisotope Enzymes And Cancer Study
Authors: Tran Luyen Van
Published: September 2017

Cancer is a pathological symptom, when abnormal cells appear in certain human tissues or organs. These cells can reproduce beyond the control of normal biological protection mechanism. Because they multiply themselves rapidly, the metabolic process is accelerated, which causes an extreme need for energy, substrate material and catalyzing enzyme. Bases on these needs, we can control the metabolic process by:  • Stopping the supply of energy.  • Stopping the supply of substrate materials to build up the cell’s structure.  • Stopping the catalysis process by breaking out the enzyme’s structure and/or deactivating the catalytic function of existing enzymes.  • Destroying the tumor cells by extraneous agents such as radiations and chemicals.  All of these methods have been studied for a long time, with a lot of resources to be spent. Although we obtained some positive result, it is nowhere closed to satisfactory. There are many reasons for this situation but the main issue is the lack of information of the processes taking place in human’s cells and body.  In this paper, we, however, would like to propose a method to break the structure and/or to deactivate the catalytic function of the enzyme by nuclear decay process.
ID:2025 Cancer Molecular Medicine: Targeting C-Myc and USP37 Interactions
Authors: Shakir Saadia , Hussain Mushtaq
Published: September 2017

Background: C-Myc is master transcriptional regulator found to be dysregulated in variety of cancers. One major ubiquitin specific protease that regulate the turnover of C-Myc is USP37. The present work aims to explore molecular interaction points between C-MYC and USP37. Secondarily, a peptide disruptor is designed for this interaction.

Methods: A composite molecular model of USP37 was generated by Modeller v9.17 using atomic coordinates of C19 domain of USP46, pleckstrin domain and atomic coordinates of USP37 generated using iterative threading modeling. After thermodynamic and structural refinement, finally selected model of USP37 was docked against atomic coordinates of C-Myc (PDBid 5I4Z) under static and dynamic state. The disruptor peptide was designed de novo and based on the hotspots of interactions between USP37 and C-Myc. Finally, the potential activity of disruptor peptide was assessed by undertaking molecular docking of peptide with USP37.

Results: Structurally, USP37 molecule resemble with a bowl shape where both C19 and pleckstrin domain were found in the central region. Near N-terminal the un annotated region was found to adopt structure homologous to the Zn finger. This denotes the possibility of USP37 interaction with DNA alone or along with c-Myc. The interaction points of USP37 and c-Myc lies at region which is exterior to both pleckstrin and C-19 domain. This binding interface contains both polar and no polar residues, taken this and sequence permutation into account a peptidyl disruptor is designed and docked against USP37. Most docking simulation results in the congregation of designed peptidyl disruptor to the targeted spatial position on USP37 molecule, suggesting its potential effectiveness.

Conclusion: The findings could be exploited in designing small molecular and peptidyl disruptor that could be used as a chemotherapeutic agent in cancers where C-Myc-USP37 interactions plays an important role in pathogenesis.
ID:2026 Investigating Molecular Interactions of EBV Oncogene, EBNA1, by Heterologous Cloning and Peptide Array System
Authors: Hussain Mushtaq , Wilson Joanna B
Published: September 2017

Background: EBNA1 is an important latent genes that is involved in the oncogenesis associated with Epstein Barr Virus. The protein is highly promiscuous in terms of its interaction with the several proteins including EBP2 and USP7, a p53 stabilizer. Herein, we aim to investigate the interactions points of molecular complexes of EBNA1, an EBV oncogene, in order to design potential therapeutic peptides.

Methods: Full length EBNA1, EBP2 and USP7 models were constructed employing iterative threading. After proper refinements of models, EBNA1 dimer was constructed employing geometrical and structural constraints. Disruptor peptide was designed and its efficiency was assessed by molecular docking and confocal microscopy. In parallel, USP7 and EBP2 molecules were expressed in pET28c expression vector and after purification by affinity chromatography, the purified proteins were probed over EBNA1 peptide array (wild type and alanine substitution array) to resolve the fine interaction details. Finally, structural conformations of intermolecular complexes were examined by molecular docking.

Results: EBNA1 forms a dimer by engaging its C-terminal DNA binding domain where a proline loop of one molecule penetrated the C-terminal tail of another monomer. A disruptor peptide designed accordingly tend to bind in that region and found to inhibit EBNA1 dimerization resulting in the cellular apoptosis as observed in confocal microscopy and MTT assay. Peptide array systems showed the binding EBNA1 at the glycine and arginine rich regions especially ranging between 325 to 375 amino acids. Similarly, USP7 interactions were noticed between 441 to 450 amino acids where Ser447 holds a key position. Interestingly, phosphorylation of this serine completely inhibits the interaction. Molecular docking analysis further verify the binding sites and their draggability.

Conclusion: The present data shows molecular interactions of EBNA1 molecules at molecular resolution. The findings can be exploited to design inhibitors to curtail molecular interactions that leads to pathological consequences.
ID:2028 PLASMA LEVELS OF MMPs AND TIMP-1 IN BLADDER CANCER PATIENTS
Authors: Yakovlev Pavel , Tetiana Ishchuk , Diana Glavachek , Olexsiy Savchuk , Tetiana Falaleeva , Ludmila Ostapchenko
Published: September 2017

Background: Urinary bladder cancer (UBC) is a common disease worldwide with high mortality rate [1]. UBC is the ninth most frequently diagnosed cancer worldwide, with highest incidence rates observed in developed countries. About 75% of bladder cancer patients are men [2]. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are diagnostic tools in oncology, liver diseases, and rheumatoid arthritis, which may prove to be a valuable prognostic tool in clinical setting [3]. The aim of the present study was to assess the prognostic power of plasma MMPs and TIMP-1 in patients with UBC of different stages.

 

Methods: This study enrolled 29 patients with UBC (27 males, 2 females), aged 52-76 years. Preoperative study for all patients included blood test, blood chemistry, urine analysis, immunogram, computer tomography, tumor biopsy. The spread of cancer was characterized based on TNM clinical classification 7th review (2009). Stage distribution of patients were as following: Stage I – 6 patients; Stage II - 5; Stage III - 7; Stage IV - 8 patients). The control group consisted of 15 healthy individuals (8 females and 22 males; aged 30-35 years). Plasma levels of MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 and TIMP-1 were determined by ELISA before the curative procedure and correlated afterwards with clinical parameters of the patient. Data analysis was conducted using Microsoft Excel 2010.

 

Results: Plasma levels of MMP-9 were significantly higher (by 1.5-2.0 times) in all patients with UBC compared to controls. Plasma level of MMP-8 in Stage III UBC patients was 1.2 times higher than in control group. The levels of MMP-1 and MMP-2 were increased compared to controls, and their elevation correlated with increase in cancer stage. Plasma level of MMP-3 in patients with bladder cancer Stage I, II and III were higher compared to controls by 1.3, 1.2 and 1.2 times respectively. High plasma levels of TIMP-1 were determined in patients with Stages III and IV, which raises question as to its role and function in advanced stage disease, as it may have a protective role in the mechanism of invasion by inhibiting extracellular matrix degradation.

Conclusion: In patients with urinary bladder cancer the plasma levels of MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 and TIMP-1 are elevated. We demonstrated that elevation pattern of MMP-1, MMP-2 and MMP-9 could serve as potential plasma marker for monitoring the cancer progression.