bluuebunny/crossref_metadata_2025 huggingface dataset:
The Morais Dictionary: Following Best Practices in a Retro-digitized Dictionary Project
Authors: Salgado Ana , Romary Laurent , Costa Rute , Tasovac Toma , Khan Anas Fahad , Ramos Margarida , Almeida Bruno , Carvalho Sara , Khemakhem Mohamed , Silva Raquel , Lehečka Boris
Published: April 2024

This article outlines essential best practices for retro-digitized dictionary projects, using the ongoing MORDigital project (DOI 10.54499/PTDC/LLT-LIN/6841/2020) as a case study. The MORDigital project focuses on digitally transforming the historically significant Portuguese Morais dictionary’s first three editions (1789, 1813, 1823). While the primary objective is to create faithful digital versions of these renowned dictionaries, MORDigital stands out by going beyond the mere adoption of established best practices. Instead, it reflects on the choices made throughout the process, providing insights into the decision-making process. The key topics emphasized include (1) the establishment of a robust data model; (2) the refinement of metadata; (3) the implementation of consistent identifiers; and (4) the enhancement of encoding techniques; additionally exploring the issue of structuring domain labelling. The article aims to contribute to the ongoing discourse on best practices in retro-digitized dictionary projects and their implications for data preservation and knowledge organization.
Faults and pitfalls that can be prevent when opening an ophthalmology center (based on the report at the Autumn Refractive Readings – 2023)
Authors: Tarasova I. V.
Published: April 2024

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Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants
Authors: You Xiaoxiao , Wang Zhongke , Wang Li , Liu Youbo , Chen Hongmei , Lan Xiaorong , Guo Ling
Published: April 2024

The construction of an antibacterial biological coating on titanium surface plays an important role in the long-term stability of oral implant restoration. Graphene oxide (GO) has been widely studied because of its excellent antibacterial properties and osteogenic activity. However, striking a balance between its biological toxicity and antibacterial properties remains a significant challenge with GO. ε-poly-L-lysine (PLL) has broad-spectrum antibacterial activity and ultra-high safety performance. Using Layer-by-layer self-assembly technology (LBL), different layers of PLL/GO coatings and GO self-assembly coatings were assembled on the surface of titanium sheet. The materials were characterized using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and contact angle test. The antibacterial properties of Porphyromonas gingivalis (P.g.) were analyzed through SEM, coated plate experiment, and inhibition zone experiment. CCK-8 was used to determine the cytotoxicity of the material to MC3T3 cells, and zebrafish larvae and embryos were used to determine the developmental toxicity and inflammatory effects of the material. The results show that the combined assembly of 20 layers of GO and PLL exhibits good antibacterial properties and no biological toxicity, suggesting a potential application for a titanium-based implant modification scheme.
Magnetic-driven hydrogel microrobots for promoting osteosarcoma chemo-therapy with synthetic lethality strategy
Authors: Tao Yining , Li Leike , Yang Xiyu , Yin Shiyu , Zhang Zhanxiang , Wang Haoyu , Pu Ruochen , Wang Zongyi , Zhang Qi , Mu Haoran , Wu Chenqiong , He Jin , Yang Liu
Published: April 2024

The advancements in the field of micro-robots for drug delivery systems have garnered considerable attention. In contrast to traditional drug delivery systems, which are dependent on blood circulation to reach their target, these engineered micro/nano robots possess the unique ability to navigate autonomously, thereby enabling the delivery of drugs to otherwise inaccessible regions. Precise drug delivery systems can improve the effectiveness and safety of synthetic lethality strategies, which are used for targeted therapy of solid tumors. MYC-overexpressing tumors show sensitivity to CDK1 inhibition. This study delves into the potential of Ro-3306 loaded magnetic-driven hydrogel micro-robots in the treatment of MYC-dependent osteosarcoma. Ro-3306, a specific inhibitor of CDK1, has been demonstrated to suppress tumor growth across various types of cancer. We have designed and fabricated this micro-robot, capable of delivering Ro-3306 precisely to tumor cells under the influence of a magnetic field, and evaluated its chemosensitizing effects, thereby augmenting the therapeutic efficacy and introducing a novel possibility for osteosarcoma treatment. The clinical translation of this method necessitates further investigation and validation. In summary, the Ro-3306-loaded magnetic-driven hydrogel micro-robots present a novel strategy for enhancing the chemosensitivity of MYC-dependent osteosarcoma, paving the way for new possibilities in future clinical applications.
Gut microbiota’s influence on erysipelas: evidence from a two-sample Mendelian randomization analysis
Authors: Bao Lijie , Wang Zehui , Wu Lidong , Luo Zhiqiang , Wang Yibing
Published: April 2024

Background

Previous studies have suggested a link between gut microbiota and skin diseases, including erysipelas, an inflammatory skin condition. Despite this, the precise nature of the relationship between erysipelas and gut microbiota remains unclear and subject to debate.

Methods

We conducted a Mendelian Randomization (MR) analysis using publicly available summary data from genome-wide association studies (GWAS) to explore the potential causal relationship between gut microbiota and erysipelas. Instrumental variables (IVs) were identified using a comprehensive set of screening methods. We then performed MR analyses primarily using the Inverse Variance Weighted (IVW) method, complemented by alternative approaches such as MR Egger, weighted median, simple mode, and weighted mode. A series of sensitivity analyses, including Cochran’s Q test, MR-Egger intercept test, Mendelian Randomization Pleiotropy RESidual Sum and Outlier (MR-PRESSO) test, and a leave-one-out test, were executed to ensure the robustness and validity of our findings.

Results

We identified potential associations between erysipelas and various gut microbiota, including Alcaligenaceae (OR 1.23; 95% CI 1.06-1.43; p=0.006), Rikenellaceae (OR 0.77; 95% CI 0.67-0.90; p=0.001), and others. Notably, associations with Actinomyces, Lachnospiraceae NC2004 group, Ruminiclostridium 9, Ruminococcaceae UCG014, Odoribacter, and Actinobacteria were also observed. Sensitivity analyses confirmed the robustness of these associations.

Conclusion

Our MR analysis suggests both potentially beneficial and harmful causal relationships between various gut microbiota and the incidence of erysipelas. This study provides new theoretical and empirical insights into the pathogenesis of erysipelas and underscores the potential for innovative preventive and therapeutic approaches.
Economic self-sufficiency (ESS) as a barrier to health self-sufficiency (HSS) for Burmese refugees in the United States: a culture-centered analysis
Authors: Kumar Rati
Published: April 2024

From displacement to resettlement, Burmese refugees bear high disease burdens; first as forced migrants escaping conflict and then as second-class citizens in host countries like the United States. This relegation of refugee bodies to the margins upon resettlement, warrants an examination of the contradictions of the process as a humanitarian enterprise yet in violation of the United Nations Sustainable Development Goal 10 of Reduced Inequalities, particularly through a health inequities lens. In the present study, the researcher conducted 15 semi-structured in-depth interviews with key stakeholders within the resettlement ecosystem of the city of Indianapolis, Indiana in the U.S., including Burmese community members, leaders, and health practitioners. Narrative data reveal an inverse relationship between the federally mandated metric of economic self-sufficiency (ESS) defined as job placement upon resettlement, and health self-sufficiency (HSS) defined as the ability to meaningfully engage with healthcare structures and resources. Participants articulate the precarious nature of such low-wage employment in predatory industries like meatpacking, requiring little to no language or vocational training and subjecting refugee employees to coercive practices including mandatory overtime. These practices are outlined as both dangerous to the health of employees and detracting from their time resources which would otherwise be allocated toward linguistic and vocational training—both required for socioeconomic mobility, as well as to meaningfully navigate labyrinthian healthcare structures. Faced with these structural constraints, Burmese community members demonstrate health self-sufficiency through a combination of treatment with over-the-counter (OTC) medications, traditional healing practices, and by activating familial and community networks as communal resources for accessing healthcare structures.
Imbalanced media portrayal: a content analysis of precision medicine in US news of 2015–2021
Authors: Peng Wei , Seo Hae Yeon , Choi Juwhan , Jia Xiaofeng
Published: April 2024

Introduction

Engaging diverse participants is vital to precision medicine (PM) but has been limited by low knowledge and concerns about various issues related to PM research. News media is an important channel of information that can shape public understanding and perception of PM. However, how PM is represented in news media has not been sufficiently understood.

Methods

The study used quantitative content analysis to evaluate the portrayal of PM in US print news media between 2015 and 2021 (N = 198). Three domains of factors related to PM portrayed in news articles were coded: (1) characteristics of PM, (2) target diseases of PM and their related characteristics, and (3) non-scientific news frames.

Results

There was considerable news coverage of the treatment benefits of PM, especially for cancer. Potential risks or concerns, non-cancer diseases, and non-treatment issues that could be important to diverse populations were less covered. News articles frequently cited scientists, patients, and government officials with different focuses on PM.

Discussion

The study highlighted the need for accurate and complete information about PM in news media for diverse participants. News media should actively explore social, ethical, and legal issues to support the engagement of diverse populations.
Oral nitrate-reducing bacteria as potential probiotics for blood pressure homeostasis
Authors: Chai Xiaofen , Liu Libing , Chen Feng
Published: April 2024

Hypertension is a leading cause of morbidity and mortality worldwide and poses a major risk factor for cardiovascular diseases and chronic kidney disease. Research has shown that nitric oxide (NO) is a vasodilator that regulates vascular tension and the decrease of NO bioactivity is considered one of the potential pathogenesis of essential hypertension. The L-arginine-nitric oxide synthase (NOS) pathway is the main source of endogenous NO production. However, with aging or the onset of diseases, the function of the NOS system becomes impaired, leading to insufficient NO production. The nitrate–nitrite–NO pathway allows for the generation of biologically active NO independent of the NOS system, by utilizing endogenous or dietary inorganic nitrate and nitrite through a series of reduction cycles. The oral cavity serves as an important interface between the body and the environment, and dysbiosis or disruption of the oral microbiota has negative effects on blood pressure regulation. In this review, we explore the role of oral microbiota in maintaining blood pressure homeostasis, particularly the connection between nitrate-reducing bacteria and the bioavailability of NO in the bloodstream and blood pressure changes. This review aims to elucidate the potential mechanisms by which oral nitrate-reducing bacteria contribute to blood pressure homeostasis and to highlight the use of oral nitrate-reducing bacteria as probiotics for oral microbiota intervention to prevent hypertension.
Prevalence and outcomes of atrial fibrillation in patients suffering prostate cancer: a national analysis in the United States
Authors: Pan Zhemin , Xu Xiao , Xu Xi , Wu Shengyong , Zhang Zhensheng , Liu Suxuan , Liu Zhijian , Tu Boxiang , Chen Chenxin , Qin Yingyi , He Jia
Published: April 2024

Purpose

Although the adverse effects of atrial fibrillation (AF) on cancers have been well reported, the relationship between the AF and the adverse outcomes in prostate cancer (PC) remains inconclusive. This study aimed to explore the prevalence of AF and evaluate the relationship between AF and clinical outcomes in PC patients.

Methods

Patients diagnosed with PC between 2008 and 2017 were identified from the National Inpatient Sample database. The trends in AF prevalence were compared among PC patients and their subgroups. Multivariable regression models were used to assess the associations between AF and in-hospital mortality, length of hospital stay, total cost, and other clinical outcomes.

Results

256,239 PC hospitalizations were identified; 41,356 (83.8%) had no AF and 214,883 (16.2%) had AF. AF prevalence increased from 14.0% in 2008 to 20.1% in 2017 (P < .001). In-hospital mortality in PC inpatients with AF increased from 5.1% in 2008 to 8.1% in 2017 (P < .001). AF was associated with adverse clinical outcomes, such as in-hospital mortality, congestive heart failure, pulmonary circulation disorders, renal failure, fluid and electrolyte disorders, cardiogenic shock, higher total cost, and longer length of hospital stay.

Conclusions

The prevalence of AF among inpatients with PC increased from 2008 to 2017. AF was associated with poor prognosis and higher health resource utilization. Better management strategies for patients with comorbid PC and AF, particularly in older individuals, are required.
Extracting geological and alteration information and predicting antimony ore based on multisource remote sensing data in Huangyangling, Xinjiang
Authors: Wang Dan , Chen Jianping , Dai Xin
Published: April 2024

Introduction: China has the world’s largest amount of antimony resources and plays a vital role in the global antimony industry. The Huangyangling area in Xinjiang belongs to the Lazhulong-Huangyangling antimony mineralization belt; this area is rich in antimony ore resources, with potential for discovery of additional ore. This high-elevation mountainous area is located in the northwestern region of the northern Tibetan Plateau, with a harsh natural environment and poor transportation access; thus, the use of traditional geological exploration methods is limited, and remote sensing technology provides unique advantages.

Methods: Using Landsat 8 OLI and Gaofen-6 (GF-6) data, we extracted stratigraphic, lithologic and fault information from the study area through information enhancement processing of the images. Based on Landsat 8 OLI, Sentinel-2 and ASTER images and the use of remote sensing image preprocessing and interference information masking, the spectral characteristics of limonite, as well as hydroxyl, carbonate, and silicified alteration minerals were utilized; the characteristics included reflection or absorption in specific spectral bands. The spectral characteristics were then combined with principal component analysis (PCA) and the band ratio method to extract the alteration information for the study area.

Results: The stratigraphy and boundaries between stratigraphic and lithologic units extracted in this study were defined, and numerous small faults were identified based on a comparison with 1:250,000 geologic maps. And the overall distribution trends of the indicators of alteration extracted in this study were located along fault zones; additionally, the hydroxyl and carbonate alteration anomalies matched well with known deposits (occurrences). Thus, the alteration information is a good indication of antimony ore bodies. Based on the geological interpretation, alteration information extraction and geochemical exploration data, four prospecting areas were identified.

Discussion: The method of extracting geological and hydrothermal alteration information by remote sensing has practical significance because it provides the possibility for geological work and mineral exploration in difficult high-elevation environments.
The syndrome of multiple bodies: the transformative impact of the onlife existence on preadolescents
Authors: Digennaro Simone
Published: April 2024

This conceptual paper explores the integration of technology and virtual spaces into preadolescents’ lives, blurring the boundaries between the digital and physical worlds. It emphasises the profound implications of this integration on various aspects, including perception, reality, and interactions. The younger generations, deeply immersed in this reality, experience a transformation in their perception of the world, well-being, identity, and selfhood due to technology’s pervasive influence. The fusion of online and offline experiences gives rise to a new form of existence, shaping a hybrid identity that can be manipulated and reconstructed in the virtual realm. However, this fluidity and transience of virtual life also pose risks and challenges to personal identity and societal ruptures. Educators face the dual challenge of effectively integrating technology into education while understanding its impact on the new generations. Social media usage influences the interlink between body and identity processes, challenging traditional notions of embodiment and raising questions about the malleability and multiplicity of identities in technologically mediated spaces. The proposed reflections seek to provide valuable guidance for educators in navigating this complex digital reality.
Apigenin and Rutaecarpine reduce the burden of cellular senescence in bone marrow stromal stem cells
Authors: Ali Dalia , Okla Meshail , Abuelreich Sarah , Vishnubalaji Radhakrishnan , Ditzel Nicholas , Hamam Rimi , Kowal Justyna M. , Sayed Ahmed , Aldahmash Abdullah , Alajez Nehad M. , Kassem Moustapha
Published: April 2024

Introduction

Osteoporosis is a systemic age-related disease characterized by reduced bone mass and microstructure deterioration, leading to increased risk of bone fragility fractures. Osteoporosis is a worldwide major health care problem and there is a need for preventive approaches.

Methods and results

Apigenin and Rutaecarpine are plant-derived antioxidants identified through functional screen of a natural product library (143 compounds) as enhancers of osteoblastic differentiation of human bone marrow stromal stem cells (hBMSCs). Global gene expression profiling and Western blot analysis revealed activation of several intra-cellular signaling pathways including focal adhesion kinase (FAK) and TGFβ. Pharmacological inhibition of FAK using PF-573228 (5 μM) and TGFβ using SB505124 (1μM), diminished Apigenin- and Rutaecarpine-induced osteoblast differentiation. In vitro treatment with Apigenin and Rutaecarpine, of primary hBMSCs obtained from elderly female patients enhanced osteoblast differentiation compared with primary hBMSCs obtained from young female donors. Ex-vivo treatment with Apigenin and Rutaecarpine of organotypic embryonic chick-femur culture significantly increased bone volume and cortical thickness compared to control as estimated by μCT-scanning.

Discussion

Our data revealed that Apigenin and Rutaecarpine enhance osteoblastic differentiation, bone formation, and reduce the age-related effects of hBMSCs. Therefore, Apigenin and Rutaecarpine cellular treatment represent a potential strategy for maintaining hBMSCs health during aging and osteoporosis.
Multivariate low-carbon scheduling of distribution network based on improved dynamic carbon emission factor
Authors: Ren Xiaofeng , Gao Hailong , Zhang Xiao , Gu Jie , Hong Lucheng
Published: April 2024

The “load-following” characteristic of the power system makes the electricity consumption behavior on the load side crucial for the low-carbon operation of the distribution network. To address this, this paper proposes an improved dynamic carbon emission factor for the distribution network, taking into account the spatiotemporal characteristics of carbon emission intensity and the integration capacity of photovoltaics (PV). Based on this, a calculation method for the carbon emissions of the distribution network load is provided. Subsequently, for commercial and industrial user scenarios, demand response models are separately constructed for commercial and industrial loads based on different driving mechanisms. Using time-of-use electricity prices as decision variables, optimization scheduling of the distribution network is carried out with the objectives of minimizing scheduling costs and carbon emissions. At the same time, a case study is conducted in an improved IEEE-33 node distribution network. The results indicate that, under the guidance of the improved dynamic carbon emission factor, load transfer can be achieved through fluctuating electricity prices, effectively reducing the scheduling costs of the distribution network, decreasing carbon emissions, and enhancing the PV integration capacity of the distribution network in different user scenarios. Finally, it is hoped that in the future, this optimization method can be widely applied, and further research can explore coordinated strategies among generation, network, load, and storage to advance the development of the power industry.
Is water replenishment an effective way to improve lake water quality? Case study in Lake Ulansuhai, China
Authors: Liu Bojun , Yang Libin , Cui Changyong , Wan Weifeng , Liang Shuntian
Published: April 2024

Lakes are an important component of the global water cycle and aquatic ecosystem. Lake water quality improvement have always been a hot topic of concern both domestically and internationally. Noncompliant outflow water quality frequently occurs, especially for lakes that rely mainly on irrigation return flow as their water source. External water replenishment to improve the water quality of lakes is gradually being recognized as a promising method, which however, is also a controversial method. Lake managers, in the case of constant controversy, hesitate about the appropriateness of lake water replenishing. Thus, taking Lake Ulansuhai in China as an example, this study aimed to construct a lake hydrodynamic and water quality model, under the constraint of multiple boundary conditions, that has sufficient simulation accuracy, and to simulate and analyze the changes in COD (Chemical Oxygen Demand) and TN (Total Nitrogen) concentrations in the lake area before and after water replenishment, and explore whether water replenishment was an effective method for improving lake water quality. The results showed that when the roughness value of Lake Ulansuhai was 0.02, the TN degradation coefficient K was 0.005/d, and the COD degradation coefficient K was 0.01/d; the simulation and measured values had the best fit, and the built model is reasonable and reliable can be used to simulate lake water quality changes. By external water replenishment lasting 140 days in the water volume of 4.925 × 108 m³, the COD and TN concentrations in Lake Ulansuhai could be stabilized at the Class V water quality requirement, which helped improve the self-purification ability of the lake area. Water replenishment was proved to be an effective method for improving the water quality of the lake, but water replenishment is only an emergency measure. Lake water replenishment is more applicable to areas with abundant water resources. External source control and internal source reduction of lake pollution and protection of lake water ecology are the main ways to improve lake water quality for water-deficient areas under the rigid constraints of water resources. In the future, key technologies for reducing and controlling pollution in irrigation areas, construction of lake digital twin platforms, and active promotion of lake legislation work should be the main research direction for managing the lake water environment.
Patterns of morphological diversification are influenced by dietary evolution in a highly species-rich lizard radiation
Authors: Ocampo Mauricio , Pincheira-Donoso Daniel , Rios Rodrigo S.
Published: April 2024

The diversification of lineages is facilitated or constrained by the simultaneous evolution of multiple components of the phenotype that interact with each other during the course of speciation. When evolutionary radiations are adaptive, lineages proliferate via the emergence of multiple phenotypic optima that underlie diversification of species across multiple ecological niches. When radiations are non-adaptive, lineage proliferation unfolds constrained by similar (or nearly identical) correlations among traits that keep phenotypic and ecological diversity across newly emerging species within a single optimum. Nature offers very few opportunities where both types of diversification occur between closely related and highly diverse lineages. The Liolaemidae family of South American lizards offers unique such opportunities given two speciose lineages that have rapidly proliferated via adaptive (Liolaemus) and non-adaptive (Phymaturus) radiations. We analyze body shape in lizards in association with type of diet (herbivory, omnivory or carnivory). In these lizards, diet types have been suggested to be linked to body size. Our results confirm this hypothesis, with three body size optima tightly linked to all three diet types when radiation is both adaptive and non-adaptive. Diet reconstruction along their evolutionary history showed that the common ancestor of Liolaemidae was likely omnivorous, which is matched by ancestral reconstruction of body size. Phylogenetic PCA revealed that herbivorous species generally have more differentiated body shape than insectivores and omnivores. Herbivorous species have evolved larger heads, shorter hindlimbs and a small difference between forelimb and hindlimb length. In contrast, omnivores and insectivores have smaller heads and longer hindlimbs. Collectively, trophic niche plays an important role in defining body shape and size across species within lineages, and the patterns of trait–ecology correlations remain consistent when lineages have diversified via adaptive and non-adaptive radiation.
Evaluation of phytoconstituents in marigold effluent for their antifungal activity against plant pathogens
Authors: Sanam Tulja , Nagaraju Umashankar , P. S Benherlal , Nerella Sridhar Goud , R Jayaramaiah. , G. G Kadalli. , V Satya Srii.
Published: April 2024

The current study placed an intense emphasis on the excess discharge of agro-based industrial effluent and the use of plant extract antimicrobials to inhibit the growth of pathogens in crop plants. An effluent (treated and untreated) from the marigold flower processing industry has been identified for the presence of volatile and semi-volatile organic compounds, and a total of 18 in treated effluent and 23 in untreated effluent were found using gas chromatography–mass spectrometry. A total of 13 classes were identified, which include carboxylic acid, phenols, esters, alkanes, alkenes, alcohols, cyanide, heterocyclic, flavonoids, aldehydes, polycyclic aromatic, cycloalkanes, and cycloalkenes. A principal component analysis with varimax rotation was applied to discern the abundance of identified compounds under each class. An in vitro antifungal bioassay was conducted using effluents at three different concentrations against plant pathogens (Alternaria alter nata, Sclerotium rolfsii, Rhizoctonia solani, Pythium aphanidermata, Fusarium oxysporum, and Colletotrichum gloeosporioides). The study proved that treated and untreated effluents clearly inhibited the growth of fungal pathogens by 10 to 32% and 37 to 92%, respectively. The findings suggest that marigold flower effluent can be a promising resource for developing new plant protection methods that are effective against pathogenic fungi.
Enrichment of type 1 innate lymphoid cells in the course of human atherosclerotic plaque development suggests contribution to atherogenesis
Authors: Pertiwi Kartika R. , Teunissen Marcel B. M. , Krebbers Gabrielle , Willems Martine C.M. , Huisman Laurens , Poelen Cindy , van der Wal Allard C. , de Boer Onno J.
Published: April 2024

Introduction

Innate lymphoid cells (ILCs) have been implicated in multiple pathologic conditions, including atherogenesis, as documented in experimental mice studies, however, their role in atherosclerosis in humans remains unexplored.

Methods

Here, we identify ILCs and their dynamics in early, advanced, and complicated human carotid- and aortic atherosclerotic plaques, using a multiplex immunohistochemical quadruple-staining technique with prototypic transcription factors T-bet, GATA3, or RORgt for identification of the ILC1, ILC2 and ILC3 subsets, respectively, in combination with lineage markers CD3, CD20/ CD79a and CD56 to exclude other lymphoid cell types. ILC subsets were quantified, and to put this in perspective, their numbers were expressed as percentage of the total number of infiltrated lymphoid cells and related to the frequency of conventional T cells, B cells, NK cells, and NKT cells.

Results

All ILC subsets were present in every different stage of atherogenesis. ILC1s were the most abundant ILC subset, and their numbers significantly increased in the course of plaque development, but paradoxically, their relative frequency was reduced because of a higher increment of T cells and B cells. The numbers of ILC2s and ILC3s also gradually increased, but this trend did not achieve significance. T cell subsets always significantly outnumbered their ILC counterparts, except for the early lesions where the proportion of ILC1s was markedly higher, albeit not significant.

Discussion

The high abundance of ILC1s in the early stages and further significant enrichment in later stages, suggest they may participate in the initiation and development of atherogenesis, and thus, may represent a novel target to prevent or treat atherosclerosis.
Arginine alleviates Clostridium perfringens α toxin-induced intestinal injury in vivo and in vitro via the SLC38A9/mTORC1 pathway
Authors: Wang Xiaohui , Zhang Tong , Li Wenli , Wang Heliang , Yan Lei , Zhang Xiaowen , Zhao Lianwen , Wang Nianxue , Zhang Beibei
Published: April 2024

Introduction

Clostridium perfringens α toxin is a main virulence factor responsible for gut damage in animals. Arginine is a functional amino acid exhibiting significant immunoregulatory activities. However, the effects and immunoregulatory mechanisms of arginine supplementation on α toxin-induced intestinal injury remain unclear.

Methods

In vivo, 256 male Arbor Acres chickens were randomly assigned to a 2×2 factorial arrangement, involving diet treatments (with or without 0.3% arginine supplementation) and immunological stress (with or without α toxin challenge). In vitro, IEC-6 cells were treated with or without arginine in the presence or absence of α toxin. Moreover, IEC-6 cells were transfected with siRNA targeting mTOR and SLC38A9 to explore the underlying mechanisms.

Results and discussion

The results showed that in vivo, arginine supplementation significantly alleviated the α toxin-induced growth performance impairment, decreases in serum immunoglobulin (Ig)A and IgG levels, and intestinal morphology damage. Arginine supplementation also significantly reduced the α toxin-induced increase in jejunal proinflammatory cytokines interleukin (IL)-1β, IL-6 and IL-17 mRNA expression. Clostridium perfringens α toxin significantly decreased jejunal mechanistic target of rapamycin (mTOR) and solute carrier family 38 member 9 (SLC38A9) mRNA expression, while arginine supplementation significantly increased mTOR and SLC38A9 mRNA expression. In vitro, arginine pretreatment mitigated the α toxin-induced decrease in cell viability and the increase in cytotoxicity and apoptosis. Arginine pretreatment also alleviated the α toxin-induced upregulation of mRNA expression of inflammation-related cytokines IL-6, C-X-C motif chemokine ligand (CXCL)10, CXCL11 and transforming growth factor-β (TGF-β), as well as apoptosis-related genes B-cell lymphoma-2 associated X protein (Bax), B-cell lymphoma-2 (Bcl-2), B-cell lymphoma-extra large (Bcl-XL) and cysteinyl aspartate specific proteinase 3 (Caspase-3) and the ratio of Bax to Bcl-2. Arginine pretreatment significantly increased the α toxin-induced decrease in mTOR, SLC38A9, eukaryotic translation initiation factor 4E (eIF4E)-binding protein 1 (4EBP1) and ribosomal protein S6 kinase (S6K) mRNA expression. Knockdown SLC38A9 and mTOR largely abrogated the positive effects of arginine pretreatment on α toxin-induced intracellular changes. Furthermore, SLC38A9 silencing abolished the increased mTOR mRNA expression caused by arginine pretreatment. In conclusion, arginine administration attenuated α toxin-induced intestinal injury in vivo and in vitro, which could be associated with the downregulation of inflammation via regulating SLC38A9/mTORC1 pathway.
The bone marrow endothelial progenitor cell response to septic infection
Authors: Shi Xin , Simms Kevin J. , Ewing Thomas J. , Lin Yuan-Ping , Chen Yi-Ling , Melvan John N. , Siggins Robert W. , Zhang Ping
Published: April 2024

Early increase in the level of endothelial progenitor cells (EPCs) in the systemic circulation occurs in patients with septic infection/sepsis. The significance and underlying mechanisms of this response remain unclear. This study investigated the bone marrow EPC response in adult mice with septic infection induced by intravenous injection (i.v.) of Escherichia coli. For in vitro experiments, sorted marrow stem/progenitor cells (SPCs) including lineage(lin)-stem cell factor receptor (c-kit)+stem cell antigen-1 (Sca-1)−, lin−c-kit+, and lin− cells were cultured with or without lipopolysaccharides (LPSs) and recombinant murine vascular endothelial growth factor (VEGF) in the absence and presence of anti-Sca-1 crosslinking antibodies. In a separate set of experiments, marrow lin−c-kit+ cells from green fluorescence protein (GFP)+ mice, i.v. challenged with heat-inactivated E. coli or saline for 24 h, were subcutaneously implanted in Matrigel plugs for 5 weeks. Marrow lin−c-kit+ cells from Sca-1 knockout (KO) mice challenged with heat-inactivated E. coli for 24 h were cultured in the Matrigel medium for 8 weeks. The marrow pool of EPCs bearing the lin−c-kit+Sca-1+VEGF receptor 2 (VEGFR2)+ (LKS VEGFR2+) and LKS CD133+VEGFR2+ surface markers expanded rapidly following septic infection, which was supported by both proliferative activation and phenotypic conversion of marrow stem/progenitor cells. Increase in marrow EPCs and their reprogramming for enhancing angiogenic activity correlated with cell-marked upregulation of Sca-1 expression. Sca-1 was coupled with Ras-related C3 botulinum toxin substrate 2 (Rac2) in signaling the marrow EPC response. Septic infection caused a substantial increase in plasma levels of IFN-γ, VEGF, G-CSF, and SDF-1. The early increase in circulating EPCs was accompanied by their active homing and incorporation into pulmonary microvasculature. These results demonstrate that the marrow EPC response is a critical component of the host defense system. Sca-1 signaling plays a pivotal role in the regulation of EPC response in mice with septic infection.
Preterm birth, preeclampsia, gestational hypertension and offspring birth weight in women with active juvenile idiopathic arthritis and healthy controls
Authors: Götestam Skorpen Carina , Lydersen Stian , Salvesen Kjell Å. , Wallenius Marianne
Published: April 2024

Objectives

There is insufficient knowledge about pregnancy outcomes in women with juvenile idiopathic arthritis (JIA). Our objective was to explore a possible association of inflammatory active JIA and pregnancy outcomes, including preterm birth, preeclampsia, gestational hypertension, and offspring gestational weight.

Methods

We linked data from the Norwegian nationwide observational register RevNatus with data from the Medical Birth Registry of Norway (MBRN) for the period 2010 to 2019. Singleton births in women with JIA (n = 181) included in RevNatus were cases. After excluding births in mothers with rheumatic inflammatory diseases, the remaining singleton births registered in MBRN, served as population controls (n = 575 798).

Results

Preterm birth was more frequent in women with active JIA (17.6%) and of equivalent frequency in women with inactive JIA (3.1%), compared to population controls (4.9%). Preeclampsia had similar rates in women with JIA and population controls while gestational hypertension was more frequent in women with active JIA (7.2%) and inactive JIA (6.9%) compared to population controls (1.7%). Abnormal fetal growth occurred in similar rates in women with JIA and population controls.

Conclusion

Having active JIA in pregnancy increased the risk for preterm birth (risk difference 12.7, 95% CI 4.7 to 25.3) and gestational hypertension (risk difference 6.2, 95% CI 1.4 to 16.8). There was no increased risk for preeclampsia or abnormal fetal growth compared to population controls.
Exploring the sex dimorphism in the expression of intestinal barrier and immune-related genes and intestinal microbiota in cage-cultured large yellow croaker (Larimichthys crocea) during the overwintering period along the Zhoushan coast
Authors: Cen Haoxiong , Li Haidong , Chen Ruiyi , Hu Weihua , Yang Yang , Li Weiye , Yin Xiaolong , Liu Bingjian , Xu Dongdong
Published: April 2024

In Zhejiang province, large yellow croakers are primarily cultured in net cages, facing significant challenges during the overwintering period such as susceptibility to cold and starvation stress. Notably, the observable sexual dimorphism in the large yellow croaker hints at the likelihood of gender differences in their responses to these environmental stresses. However, the potential sex-specific adaptive changes during overwintering remain unexplored. To gain deeper insights, we investigated the expression of intestinal barrier-related genes, immune responses, and changes in intestinal microbiota during the overwintering period in males and females separately. The results revealed a more pronounced loss of body weight in females than that in males. In male intestines, there was a significant decrease in the expression of intestinal barrier-related genes (arp2/3, occludin, and zo1), contrasting with a significant increase in females. The expression of TLR1, TLR3, TLR7, TLR9, MyD88, and NF-κB genes in the intestines of female fish decreased significantly in March compared to November, while the opposite trend was observed in male fish. However, in the liver, TLR1, TLR3, TLR7, TLR9, MyD88, and NF-κB genes expression were both decreased significantly in males and females. In the male intestines, there was a significant increase in the expression of inflammatory cytokine genes (IL-1β and IL-6). In the females, IL-1β gene expression significantly decreased, while IL-6 expression increased significantly. The expression of IL-10 genes decreased in both males and females. In the liver, both the males and females exhibited a significant increase in the expression of IL-1β and IL-6 genes. Further analysis revealed greater susceptibility of male intestinal microbiota diversity during the overwintering period. Firmicutes’ relative abundance exhibited opposing changes between the males and females, and Proteobacteria abundance, driven by a significant increase in Vibrio bacteria, significantly increased in the males. In conclusion, the overwintering period may compromise the structural integrity of male fish intestines, reducing their immune function. Additionally, the response strategy of the intestinal microbiota differs between sexes. The findings provide crucial insights for crafting effective strategies and management decisions in cage-cultured large yellow croaker during the overwintering period, as well as offering theoretical references for monosex aquaculture.
Investigation of conventional and ultrasonic vibration-assisted turning of hardened steel using a coated carbide tool
Authors: Ghule Govind S. , Sanap Sudarshan , Chinchanikar Satish , Cep Robert , Kumar Ajay , Bhave Suresh Y. , Kumar Rakesh , Altarazi Faisal
Published: April 2024

This study compares conventional turning (CT) and ultrasonic vibration-assisted turning (UVAT) in machining hardened AISI 52100 steel (62 HRC) with a PVD-coated TiAlSiN carbide tool. UVAT experiments, utilizing an ultrasonic frequency of 20 kHz and vibration amplitude of 20 µm, varied the cutting speed, feed, and depth of cut. Remarkably, UVAT reduced tool wear, extending tool longevity. Surprisingly, power consumption showed no significant differences between CT and UVAT. Mathematical models based on experimental data highlight the substantial impact of the cutting speed on tool wear, followed closely by the depth of cut. For power consumption, the depth of cut took precedence, with the cutting speed and feed rate playing pronounced roles in UVAT. This emphasizes the potential for further research on machinability, particularly exploring different vibration directions on the tool in feed, tangential, and radial aspects.
Regulation of the growth performance and the gastrointestinal microbiota community by the addition of defective pear fermentation to feed of small-tailed Han sheep
Authors: Peng Hongxin , Chen Pinpin , Guo Rui , Zhou Zheng , Chen Yafei , Xu Ping , Su Huawei , Xiao Yuncai , Jiang Hui
Published: April 2024

This study investigated the effects of defective pear fermentation (DPF) diets on growth performance and gastrointestinal microbial communities in 60 healthy male small-tailed Han sheep, aged 90 days. The sheep were randomly divided into four groups, each consisting of three replicates with five sheep per replicate. Initially, all groups received a basal diet for seven days during the adaptation stage. Subsequently, for 60 days, group C (control) was fed a basal diet, group X received a basal diet with 2% DPF, group Y had a basal diet with 4% DPF, and group Z was fed a basal diet with 6% DPF. The results indicated that group Y experienced a significant increase in average daily gain (ADG) and average daily feed intake (ADFI). The addition of DPF significantly elevated the levels of GSH-Px and notably reduced MDA content compared to group C. Analysis of gastrointestinal microbiota showed that groups receiving DPF had increased relative abundances of Lachnospiraceae_NK3A20_group, norank_f p-2534-18B5_gut_group, Acetitomaculum, Actinobacteriota, Bacteroidota and Ruminococcus_gauvreauii_group, and decreased abundances of Proteobacteria, Prevotella, Staphylococcus, and Psychrobacter compared to group C. Group X exhibited the highest relative abundance of Olsenella, while group Y showed a significant increase in unclassified_f Lachnospiraceae compared to the other groups. Bacterial function prediction indicated that pathways related to energy metabolism were more prevalent in group X and Y. This study preliminarily confirms the feasibility of using DPF as feed additives, providing a foundation for further research and evaluation of DPF's application in animal production.
The DmsABC S-oxide reductase is an essential component of a novel, hypochlorite-inducible system of extracellular stress defense in Haemophilus influenzae
Authors: Nasreen Marufa , Ellis Daniel , Hosmer Jennifer , Essilfie Ama-Tawiah , Fantino Emmanuelle , Sly Peter , McEwan Alastair G. , Kappler Ulrike
Published: April 2024

Defenses against oxidative damage to cell components are essential for survival of bacterial pathogens during infection, and here we have uncovered that the DmsABC S-/N-oxide reductase is essential for virulence and in-host survival of the human-adapted pathogen, Haemophilus influenzae. In several different infection models, H. influenzae ΔdmsA strains showed reduced immunogenicity as well as lower levels of survival in contact with host cells. Expression of DmsABC was induced in the presence of hypochlorite and paraquat, closely linking this enzyme to defense against host-produced antimicrobials. In addition to methionine sulfoxide, DmsABC converted nicotinamide- and pyrimidine-N-oxide, precursors of NAD and pyrimidine for which H. influenzae is an auxotroph, at physiologically relevant concentrations, suggesting that these compounds could be natural substrates for DmsABC. Our data show that DmsABC forms part of a novel, periplasmic system for defense against host-induced S- and N-oxide stress that also comprises the functionally related MtsZ S-oxide reductase and the MsrAB peptide methionine sulfoxide reductase. All three enzymes are induced following exposure of the bacteria to hypochlorite. MsrAB is required for physical resistance to HOCl and protein repair. In contrast, DmsABC was required for intracellular colonization of host cells and, together with MtsZ, contributed to resistance to N-Chlorotaurine. Our work expands and redefines the physiological role of DmsABC and highlights the importance of different types of S-oxide reductases for bacterial virulence.
Nematicidal glycosylated resorcylic acid lactones from the fungus Pochonia chlamydosporia PC-170 and their key biosynthetic genes
Authors: Li Zeyu , Luo Ning , Zhang Wenwen , Khan Raja Asad Ali , Ling Jian , Zhao Jianlong , Yang Yuhong , Mao Zhenchuan , Xie Bingyan , Zhou Ligang , Li Yan
Published: April 2024

Chemical study of the nematicidal biocontrol fungus Pochonia chlamydosporia PC-170 led to discovery of six resorcylic acid lactones (RALs), including three nematicidal glycosylated RALs, monocillin VI glycoside (1), colletogloeolactone A (2) and monocillin II glycoside (3), and three antibacterial non-glycosylated RALs, monocillin VI (4), monocillin IV (5) and monocillin II (6). The planar structure of the new compound monocillin VI glycoside (1) was elucidated using HRESIMS and NMR data, and its monosaccharide configuration was further determined through sugar hydrolysis experiment and GC–MS analysis method. Furthermore, their two biosynthetic-related PKS genes, pchE and pchI, were identified through the gene knockout experiment. The glycosylated RALs 1–3 exhibited nematicidal activity against Meloidogyne incognita, with LC50 values of 94, 152 and 64 μg/mL, respectively, and thus had great potential in the development of new nematicidal natural products to control M. incognita in the future.