bluuebunny/crossref_metadata_2025 huggingface dataset:
Tracing IgE-Producing Cells in Allergic Patients
Authors: Eckl-Dorna Julia , Villazala-Merino Sergio , Campion Nicholas James , Byazrova Maria , Filatov Alexander , Kudlay Dmitry , Karsonova Antonina , Riabova Ksenja , Khaitov Musa , Karaulov Alexander , Niederberger-Leppin Verena , Valenta Rudolf
Published: August 2019

Immunoglobulin E (IgE) is the key immunoglobulin in the pathogenesis of IgE associated allergic diseases affecting 30% of the world population. Recent data suggest that allergen-specific IgE levels in serum of allergic patients are sustained by two different mechanisms: inducible IgE production through allergen exposure, and continuous IgE production occurring even in the absence of allergen stimulus that maintains IgE levels. This assumption is supported by two observations. First, allergen exposure induces transient increases of systemic IgE production. Second, reduction in IgE levels upon depletion of IgE from the blood of allergic patients using immunoapheresis is only temporary and IgE levels quickly return to pre-treatment levels even in the absence of allergen exposure. Though IgE production has been observed in the peripheral blood and locally in various human tissues (e.g., nose, lung, spleen, bone marrow), the origin and main sites of IgE production in humans remain unknown. Furthermore, IgE-producing cells in humans have yet to be fully characterized. Capturing IgE-producing cells is challenging not only because current staining technologies are inadequate, but also because the cells are rare, they are difficult to discriminate from cells bearing IgE bound to IgE-receptors, and plasma cells express little IgE on their surface. However, due to the central role in mediating both the early and late phases of allergy, free IgE, IgE-bearing effector cells and IgE-producing cells are important therapeutic targets. Here, we discuss current knowledge and unanswered questions regarding IgE production in allergic patients as well as possible therapeutic approaches targeting IgE.
Glutamate at the Vertebrate Neuromuscular Junction: From Modulation to Neurotransmission
Authors: Colombo Maria Nicol , Francolini Maura
Published: August 2019

Although acetylcholine is the major neurotransmitter operating at the skeletal neuromuscular junction of many invertebrates and of vertebrates, glutamate participates in modulating cholinergic transmission and plastic changes in the last. Presynaptic terminals of neuromuscular junctions contain and release glutamate that contribute to the regulation of synaptic neurotransmission through its interaction with pre- and post-synaptic receptors activating downstream signaling pathways that tune synaptic efficacy and plasticity. During vertebrate development, the chemical nature of the neurotransmitter at the vertebrate neuromuscular junction can be experimentally shifted from acetylcholine to other mediators (including glutamate) through the modulation of calcium dynamics in motoneurons and, when the neurotransmitter changes, the muscle fiber expresses and assembles new receptors to match the nature of the new mediator. Finally, in adult rodents, by diverting descending spinal glutamatergic axons to a denervated muscle, a functional reinnervation can be achieved with the formation of new neuromuscular junctions that use glutamate as neurotransmitter and express ionotropic glutamate receptors and other markers of central glutamatergic synapses. Here, we summarize the past and recent experimental evidences in support of a role of glutamate as a mediator at the synapse between the motor nerve ending and the skeletal muscle fiber, focusing on the molecules and signaling pathways that are present and activated by glutamate at the vertebrate neuromuscular junction.
Modeling of Multivalent Ligand-Receptor Binding Measured by kinITC
Authors: Erlekam Franziska , Igde Sinaida , Röblitz Susanna , Hartmann Laura , Weber Marcus
Published: August 2019

In addition to the conventional Isothermal Titration Calorimetry (ITC), kinetic ITC (kinITC) not only gains thermodynamic information, but also kinetic data from a biochemical binding process. Moreover, kinITC gives insights into reactions consisting of two separate kinetic steps, such as protein folding or sequential binding processes. The ITC method alone cannot deliver kinetic parameters, especially not for multivalent bindings. This paper describes how to solve the problem using kinITC and an invariant subspace projection. The algorithm is tested for multivalent systems with different valencies.
Tris(2-Methoxyphenyl)Bismuthine Polymorphism Characterized by Nuclear Quadrupole Resonance Spectroscopy
Authors: Scharfetter Hermann , Fischer Roland , Krassnig Paul , Thonhofer Martin , Theyer Felix , Gösweiner Christian
Published: August 2019

Based on the previous identification of metastable polymorphs in crystalline triphenylbismuth by nuclear quadrupole resonance spectroscopy (NQRS), the potential formation of similar phases was studied in Tris(2-Methoxyphenyl)Bismuthine. To this end, commercial samples with known NQRS properties were molten and re-crystallized at different speeds (shock freezing in different coolants versus slow cooling inside of a heater). In all recrystallization products we have identified a new crystal phase which has not been observed after synthesis from a solution. The new crystallographic structure has been confirmed by X-ray diffraction (XRD). The newly isolated polymorph crystallizes in the monoclinic space group P2(1)/c with only one molecule in the asymmetric unit and consequently only one 5/2-7/2 transition is observed at 88.75 MHz at 310 K. In contrast, the two transitions at 89.38 and 89.29 MHz for the well-known trigonal polymorph originate from two crystallographically distinct molecules of Tris(2-methoxy-Phenyl)Bismuthine in the asymmetric unit. Additional relaxometric NQRS shows distinctly different T2 relaxation times for the new polymorph when compared to the original samples. Additional phase transitions could not be observed during temperature sweeps between 153 K and 323 K.
Liver Fibrosis Assessment with Diffusion-Weighted Imaging: Value of Liver Apparent Diffusion Coefficient Normalization Using the Spleen as a Reference Organ
Authors: Shin Min Ki , Song Ji Soo , Hwang Seung Bae , Hwang Hong Pil , Kim Young Jun , Moon Woo Sung
Published: August 2019

Liver fibrosis staging is of great clinical importance because it is used to assess the severity of the underlying chronic liver disease. Among various imaging-based methods, apparent diffusion coefficient (ADC) measurement using diffusion-weighted imaging (DWI) has the potential to be used as an imaging biomarker for liver fibrosis assessment. In this study, we investigated the usefulness of liver ADC normalization using the spleen as a reference organ in liver fibrosis staging with 66 patients who underwent liver magnetic resonance imaging (MRI), transient elastography (TE), and surgical resection of a hepatic mass. ADC values of the liver (ADCliver) and spleen were analyzed, and the spleen was used for ADCliver normalization (nADCliver). ADCliver showed a weak negative correlation with TE (r = −0.246; p = 0.047) and fibrosis stage (r = −0.269; p = 0.029), while n ADCliver showed a moderate negative correlation with TE (r = −0.504; p < 0.001) and fibrosis stage (r = −0.579; p < 0.001). AUC values for nADCliver (0.777–0.875) were higher than those for ADCliver for each stage of fibrosis (0.596–0.713, p = 0.037–0.157). AUC values for TE (0.726–0.884) and nADCliver were not statistically different. In conclusion, normalized liver ADC can be useful in diagnosing liver fibrosis stage in patients with variable DWI acquisitions.
Efficiency Analysis and Improvement of an Intelligent Transportation System for the Application in Greenhouse
Authors: Zhang Tianfan , Zhou Weiwen , Meng Fei , Li Zhe
Published: August 2019

In view of the future lack of human resources due to the aging of the population, the automatic, Intelligent Mechatronic Systems (IMSs) and Intelligent Transportation Systems (ITSs) have broad application prospects. However, complex application scenarios and limited open design resources make designing highly efficient ITS systems still a challenging task. In this paper, the optimal load factor solving solution is established. By converting the three user requirements including working distance, time and load into load-related factors, the optimal result can be obtained among system complexity, efficiency and system energy consumption. A specialized visual navigation and motion control system has been proposed to simplify the path planning, navigation and motion control processes and to be accurately calculated in advance, thereby further improving the efficiency of the ITS system. The validity of the efficiency calculation formula and navigation control method proposed in this paper is verified. Under optimal conditions, the actual working mileage is expected to be 99.7%, and the energy consumption is 83.5% of the expected value, which provides sufficient redundancy for the system. In addition, the individual ITS reaches the rated operating efficiency of 95.86%; in other words, one ITS has twice the ability of a single worker. This proves the accuracy and efficiency of the designed ITS system.
Effect of Annealing Environment on the Performance of Sol–Gel-Processed ZrO2 RRAM
Authors: Ha Seunghyun , Lee Hyunjae , Lee Won-Yong , Jang Bongho , Kwon Hyuk-Jun , Kim Kwangeun , Jang Jaewon
Published: August 2019

We investigate the annealing environment effect on ZrO2-based resistive random-access memory (RRAM) devices. Fabricated devices exhibited conventional bipolar-switching memory properties. In particular, the vacuum-annealed ZrO2 films exhibited larger crystallinity and grain size, denser film, and a relatively small quantity of oxygen vacancies compared with the films annealed in air and N2. These led to a decrease in the leakage current and an increase in the resistance ratio of the high-resistance state (HRS)/low-resistance state (LRS) and successfully improved non-volatile memory properties, such as endurance and retention characteristics. The HRS and LRS values were found to last for 104 s without any significant degradation.
Design and Implementation of CPU & FPGA Co-Design Tester for SDN Switches
Authors: Jiang Yue , Chen Hongyi , Yang Xiangrui , Sun Zhigang , Quan Wei
Published: August 2019

The southbound protocol of Software Defined Networking (SDN) enables the direct access into SDN switches which accelerates the innovation and deployment of network functions in the data plane. Correspondingly, SDN switches that support the new southbound protocol and provide high performance are developed continuously. Therefore, there is an increasing need for testing tools to test such equipment in terms of protocol correctness and performance. However, existing tools have deficiencies in flexibility for verifying the novel southbound protocol, time synchronization between the two planes, and supporting more testing functions with less resource consumption. In this paper, we present the concept of CPU & FPGA co-design Tester (CFT) for SDN switches, which provides flexible APIs for test cases of the control plane and high performance for testing functions in the data plane. We put forward an efficient scheduling algorithm to integrate the control plane and the data plane into a single pipeline which fundamentally solves the time asynchronization between these two planes. Due to the reconfigurable feature of our proposed pipeline, it becomes possible to perform different testing functions in one pipeline. Through a prototype implementation and evaluation, we reveal that the proposed CFT can verify the protocol correctness of SDN switches on the control plane while providing no-worse performance for tests on the data plane compared with commercial testers.
A Single Transformer for Active Cell Equalization Method of Lithium-Ion Batteries with Two Times Fewer Secondaries than Cells
Authors: Farzan Moghaddam Ali , Van den Bossche Alex
Published: August 2019

In this paper, the concept of active cell-balancing technique, by using a multiple-outputs double-forward converter for lithium-ion (Li-ion) batteries, is investigated. It controls two times more cells than secondaries, and it equalizes eight cells in a series. In this method, four secondaries can reasonably be wound with the same back electromotive force (EMF). This means a low pin count on the transformer and a low bill of materials (BOM). The bridge uses four N-channel MOSFETs as switches, which means two times fewer transistors than cells, resulting in fewer switching losses. This scheme is applied for controlling the minimum voltage among the cells of the lithium-ion battery. It uses a multi-winding transformer based on a forward double converter structure. Conventional schemes using a multi-winding transformer for electric vehicles (EVs) require an equal number of secondaries per cell. This scheme requires one secondary for two adjacent cells, thus the number of secondaries is reduced by a factor of two. Also, the redistribution of charge from a high cell to a low cell does not require many switching components and little intelligence to determine low cell voltage detection. The basic principle of this method is to use the overall battery pack voltage as a reference to supply individual cells, using a forward converter containing a transformer with a well-chosen winding ratio. The experimental and simulation results are performed to verify the feasibility of the proposed system.
Design and Performance Analysis of a Stand-alone PV System with Hybrid Energy Storage for Rural India
Authors: Javed Kashif , Ashfaq Haroon , Singh Rajveer , Hussain S.M. Suhail , Ustun Taha Selim
Published: August 2019

The operations of domestic stand-alone Photovoltaic (PV) systems are mostly dependent on storage systems due to changing weather conditions. For electrical energy storage, batteries are widely used in stand-alone PV systems. The performance and life span of batteries depend on charging/discharging cycles. Fluctuation in weather conditions causes batteries to charge/discharge quite often, which decreases the operational life and increases the maintenance cost. This paper proposes a domestic stand-alone PV system with Hybrid Energy Storage System (HESS) that is a combination of battery and supercapacitor. A new Fuzzy Logic Control Strategy (FHCS) is implemented to control the power flow of the battery and supercapacitor. Simulation studies are performed with real data collected in Sultanpur, India to investigate the proposed system’s performance (Latitude [N] 26.29 and Longitude [E] 82.08). The results show that FHCS successfully controls the power flow of HESS components to increase the system efficiency. The developed system is validated to provide an effective alternative that would enhance the battery life span and reduce the system maintenance cost. While considering the prohibitive upfront costs for rural systems, such an improvement helps to electrify more underserved communities.
Study of An Innovative Approach of Roof Presplitting for Gob-Side Entry Retaining in Longwall Coal Mining
Authors: Zhang Xingyu , Chen Liang , Gao Yubing , Hu Jinzhu , Yang Jun , He Manchao
Published: August 2019

Gob-side entry retaining (GER) is a hot issue with regard to saving resources and reducing the drivage ratio in longwall mining. This paper investigates an innovative approach of roof presplitting for gob-side entry retaining (RPGER). RPGER uses the directional cumulative blasting to split the roof in advance. The rock roof within the presplitting range caves in gob after mining. The caved gangue can become the natural rib of the gob-side entry and expands to be the natural supporting body for resisting the upper roof movement. A numerical model of RPGER was established by the discrete element method (DEM), which showed that the supporting effect by the expanded gangue was well functioning. The gob-side entry was in pressure-relief surroundings and featured in the lesser deformation. The roof presplitting design method was presented and validated with a field test. The test illustrated that RPGER reduced the mining pressure on the retained entry side. The expanded gangue on the entry side was gradually compacted. It is the compaction process that played the role of reliving mining pressure, and the compacted gangue became the effective rib of the gob-side entry. The retained entry in the pressure-relief surroundings would stabilize a lagging distance behind the working face. The gob-side entry after stabilization met the entry retaining and the safety production requirements. This work illustrates the mechanism of RPGER and validates its feasibility and efficiency.
Study on Flow and Heat Transfer Characteristics of Porous Media in Engine Particulate Filters Based on Lattice Boltzmann Method
Authors: Fu Jiale , Zhang Tiechen , Li Menghan , Li Su , Zhong Xianglin , Liu Xiaori
Published: August 2019

To investigate the laminar flow characteristics of porous media in the inner core of engine particulate filters, a two-dimensional lattice Boltzmann–Cellular Automata (LB–CA) probabilistic model is used to simulate the flow characteristics of porous media. The variation of dimensionless permeability of various numerical structures on pore scale with Reynolds number is analyzed, and the heat transfer as well as particle filtration are considered. The results show that the flow law of different structures obeys Darcy law under the condition of low Reynolds number (Re < 1). The dimensionless permeability coefficient of the ordered structure is significantly higher than that of the disordered structure; however. the filtration efficiency of the ordered structure decreases. With the increase of Reynolds number, the heat transfer increases. Furthermore, it is found that the particle size has a great influence on the filtration efficiency.
Ectomycorrhizal Community on Norway Spruce Seedlings Following Bark Beetle Infestation
Authors: Veselá Petra , Vašutová Martina , Hofmannová Karolína , Edwards-Jonášová Magda , Cudlín Pavel
Published: August 2019

Ectomycorrhizal (ECM) fungi importantly influence seedling growth, nutrition, and survival and create an extensive mycelial network interconnecting tree species and enabling resource redistribution. Due to their symbiotic relationship with trees, they are impacted by forest disturbances, which are of increasing relevance due to climate change. The effect of disturbance on seedling colonization and their morphology is still largely unknown. Seedling growth parameters and the ECM fungal assemblage on the roots of Norway spruce (Picea abies (L.) H. Karst.) seedlings were assessed in mature spruce forests attacked and destroyed by bark beetle and in a mature non-attacked forest as a reference. We did not detect significant differences in number of ECM species on seedling roots among forest types, but ECM species composition changed; Tylospora fibrillosa (Burt) Donk, Meliniomyces variabilis Hambl. & Sigler, and Phialocephala fortinii C.J.K. Wang & H.E. Wilcox were characteristic species in the forest destroyed by bark beetle, whereas Lactarius, Cortinarius, and Russula were in the mature forest. Forest type further significantly influenced the height, root length, and root collar thickness of seedlings and the proportion of exploration types of mycorrhizae.
Genome-Wide Analysis of NAC Gene Family in Betula pendula
Authors: Chen Song , Lin Xin , Zhang Dawei , Li Qi , Zhao Xiyang , Chen Su
Published: August 2019

NACs (NAM, ATAF1/2, and CUC2) are plant-specific transcription factors that play diverse roles in various plant developmental processes. In this study, we identified the NAC gene family in birch (Betula pendula) and further analyzed the function of BpNACs. Phylogenetic analysis reveals that the 114 BpNACs can be divided into seven subfamilies. We investigated the expression levels of these BpNACs in different tissues of birch including roots, xylem, leaves, and flowers, and the results showed that the BpNACs seem to be expressed higher in xylem and roots than leaves and flowers. In addition to tissue-specific expression analysis, we investigated the expression of BpNACs under low-temperature stress. A total of 21 BpNACs were differentially expressed under low-temperature stress, of which 17 were up-regulated, and four were down-regulated. Using the gene expression data, we reconstructed the gene co-expression network for the 21 low-temperature-responsive BpNACs. In conclusion, our results provide insight into the evolution of NAC genes in the B. pendula genome, and provide a basis for understanding the molecular mechanism for BpNAC-mediated cold responses in birch.
Weak Apical Control of Swiss Stone Pine (Pinus cembra L.) May Serve as a Protection against Environmental Stress above Treeline in the Central European Alps
Authors: Oberhuber Walter , Geisler Theresa Andrea , Bernich Fabio , Wieser Gerhard
Published: August 2019

At the treeline in the Central European Alps, adverse climate conditions impair tree growth and cause krummholz formation of Swiss stone pine (Pinus cembra L.). Multi-stemmed trees (tree clusters) are frequently found in the treeline ecotone and are generally thought to originate from seed caches (multiple genets) of the European nutcracker (N. caryocatactes) or due to repeated damage of the leader shoot by browsing or mechanical stress (single genet). Additionally, lack of apical control can lead to upward bending of lateral branches, which may obscure single-genet origin if the lower branching points are overgrown by vegetation and the humus layer. The multi-stemmed growth form may serve as a means of protection against extreme environmental stress during winter, especially at wind-exposed sites, because leeward shoots are protected from, e.g., ice particle abrasion and winter desiccation. The aims of this study therefore were to analyze in an extensive field survey: (i) whether weak apical control may serve as a protection against winter stress; and (ii) to what extent the multi-stemmed growth form of P. cembra in the krummholz zone is originating from a single genet or multiple genets. To accomplish this, the growth habit of P. cembra saplings was determined in areas showing extensive needle damage caused by winter stress. Multi-stemmed saplings were assigned to single and multiple genets based on determination of existing branching points below the soil surface. The findings revealed that upward bending of lateral branches could protect saplings against winter stress factors, and, although multi-stemmed P. cembra trees were primarily found to originate from multiple genets (most likely seed caches), about 38% of tree clusters originated from upward bending of (partially) buried branches. The results suggest that weak apical control of P. cembra in the sapling stage might be an adaptation to increase survival rate under severe climate conditions prevailing above treeline during winter.
Enhanced Stability and Bioactivity of Curcuma comosa Roxb. Extract in Electrospun Gelatin Nanofibers
Authors: Chiu Chi-Ming , Nootem Jukkrit , Santiwat Thanapich , Srisuwannaket Choladda , Pratumyot Kornkanya , Lin Wei-Chao , Mingvanish Withawat , Niamnont Nakorn
Published: August 2019

Electrospun fiber can be used as a carrier for releasing active ingredients at the target site to achieve the effects of drug treatment. The objectives of this research work were to study suitable conditions for producing electrospun gelatin fiber loaded with crude Curcuma comosa Roxb. extract (CE) and to study antioxidant, anti-tyrosinase and anti-bacterial activities and its freeze–thaw stability as well. To achieve optimal conditions for producing electrospun gelatin fiber, the concentration of gelatin was adjusted to 30% w/v in a co-solvent system of acetic acid/water (9:1 v/v) with a feed rate of 3 mL/h and an applied voltage of 15 kV. The lowest percent loading of 5% (w/v) CE in gelatin nanofiber exhibited the highest DPPH radical scavenging activity of 94% and the highest inhibition of tyrosinase enzyme of 35%. Moreover, the inhibition zones for antibacterial activities against S. aureus and S. epidermidis were 7.77 ± 0.21 and 7.73 ± 0.12 mm, respectively. The freeze–thaw stability of CE in electrospun gelatin nanofiber was significantly different (p < 0.05) after the 4th cycle as compared to CE. Electrospun gelatin nanofiber containing CE also showed the capacity of the release of bioactive ingredients possessing anti-oxidant properties and, therefore, it could potentially be used for face masks.
Jute Based Bio and Hybrid Composites and Their Applications
Authors: Ashraf Muhammad Ahsan , Zwawi Mohammed , Taqi Mehran Muhammad , Kanthasamy Ramesh , Bahadar Ali
Published: August 2019

The popularity of jute-based bio and hybrid composites is mainly due to an increase in environmental concerns and pollution. Jute fibers have low cost, high abundance, and reasonable mechanical properties. Research in all-natural fibers and composites have increased exponentially due to the environment concerns of the hazards of synthetic fibers-based composites. Jute based bio and hybrid composites have been extensively used in number of applications. Hybrid jute-based composites have enhanced mechanical and physical properties, reasonably better than jute fiber composites. A detailed analysis of jute-based bio and hybrid composites was carried out in this review. The primary aim of this review paper is to provide a critical analysis and to discuss all recent developments in jute-based composites. The content covers different aspects of jute-based composites, including their mechanical and physical properties, structure, morphology, chemical composition, fiber modification techniques, surface treatments, jute based hybrid composites, limitations, and applications. Jute-based composites are currently being used in a vast number of applications such as in textiles, construction, cosmetics, medical, packaging, automobile, and furniture industries.
Comprehensive Nutrition Review of Grain-Based Muesli Bars in Australia: An Audit of Supermarket Products
Authors: Curtain Felicity , Grafenauer Sara
Published: August 2019

Muesli bars are consumed by 16% of children, and 7.5% of adults, and are classified as discretionary in Australian Dietary Guidelines, containing “higher fat and added sugars” compared with core food choices. This study aimed to provide a nutritional overview of grain-based muesli bars, comparing data from 2019 with 2015. An audit of muesli bars, grain-based bars, and oat slices was undertaken in January 2019 (excluding fruit, nut, nutritional supplement, and breakfast bars) from the four major supermarkets in metropolitan Sydney. Mean and standard deviation was calculated for all nutrients on-pack, including whole grain per serve and per 100g. Health Star Rating (HSR) was calculated if not included on-pack. Of all bars (n = 165), 63% were ≤ 600 kJ (268–1958 kJ), 12% were low in saturated fat, 56% were a source of dietary fibre, and none were low in sugar. Two-thirds (66%) were whole grain (≥8 g/serve), with an average of 10 g/serve, 16% of the 48 g Daily Target Intake. HSR featured on 63% of bars (average 3.2), with an overall HSR of 2.7. Compared to 2015, mean sugars declined (26.6 g to 23.7 g/100 g; p < 0.001), and 31% more bars were whole grain (109 up from 60 bars). Although categorised as discretionary, there were significant nutrient differences across grain-based muesli bars. Clearer classification within policy initiatives, including HSR, may assist consumers in choosing products high in whole grain and fibre at the supermarket shelf.
Affinity Purification of NF1 Protein–Protein Interactors Identifies Keratins and Neurofibromin Itself as Binding Partners
Authors: Carnes Rachel M. , Kesterson Robert A. , Korf Bruce R. , Mobley James A. , Wallis Deeann
Published: August 2019

Neurofibromatosis Type 1 (NF1) is caused by pathogenic variants in the NF1 gene encoding neurofibromin. Definition of NF1 protein–protein interactions (PPIs) has been difficult and lacks replication, making it challenging to define binding partners that modulate its function. We created a novel tandem affinity purification (TAP) tag cloned in frame to the 3’ end of the full-length murine Nf1 cDNA (mNf1). We show that this cDNA is functional and expresses neurofibromin, His-Tag, and can correct p-ERK/ERK ratios in NF1 null HEK293 cells. We used this affinity tag to purify binding partners with Strep-Tactin®XT beads and subsequently, identified them via mass spectrometry (MS). We found the tagged mNf1 can affinity purify human neurofibromin and vice versa, indicating that neurofibromin oligomerizes. We identify 21 additional proteins with high confidence of interaction with neurofibromin. After Metacore network analysis of these 21 proteins, eight appear within the same network, primarily keratins regulated by estrogen receptors. Previously, we have shown that neurofibromin levels negatively regulate keratin expression. Here, we show through pharmacological inhibition that this is independent of Ras signaling, as the inhibitors, selumetinib and rapamycin, do not alter keratin expression. Further characterization of neurofibromin oligomerization and binding partners could aid in discovering new neurofibromin functions outside of Ras regulation, leading to novel drug targets.
The Conserved Non-Coding Sequence 2 (CNS2) Enhances CD69 Transcription through Cooperation between the Transcription Factors Oct1 and RUNX1
Authors: Fontela Miguel G. , Notario Laura , Alari-Pahissa Elisenda , Lorente Elena , Lauzurica Pilar
Published: August 2019

The immune regulatory receptor CD69 is expressed upon activation in all types of leukocytes and is strongly regulated at the transcriptional level. We previously described that, in addition to the CD69 promoter, there are four conserved noncoding regions (CNS1-4) upstream of the CD69 promoter. Furthermore, we proposed that CNS2 is the main enhancer of CD69 transcription. In the present study, we mapped the transcription factor (TF) binding sites (TFBS) from ChIP-seq databases within CNS2. Through luciferase reporter assays, we defined a ~60 bp sequence that acts as the minimum enhancer core of mouse CNS2, which includes the Oct1 TFBS. This enhancer core establishes cooperative interactions with the 3′ and 5′ flanking regions, which contain RUNX1 BS. In agreement with the luciferase reporter data, the inhibition of RUNX1 and Oct1 TF expression by siRNA suggests that they synergistically enhance endogenous CD69 gene transcription. In summary, we describe an enhancer core containing RUNX1 and Oct1 BS that is important for the activity of the most potent CD69 gene transcription enhancer.
Viral Metagenomics in the Clinical Realm: Lessons Learned from a Swiss-Wide Ring Trial
Authors: Junier Thomas , Huber Michael , Schmutz Stefan , Kufner Verena , Zagordi Osvaldo , Neuenschwander Stefan , Ramette Alban , Kubacki Jakub , Bachofen Claudia , Qi Weihong , Laubscher Florian , Cordey Samuel , Kaiser Laurent , Beuret Christian , Barbié Valérie , Fellay Jacques , Lebrand Aitana
Published: August 2019

Shotgun metagenomics using next generation sequencing (NGS) is a promising technique to analyze both DNA and RNA microbial material from patient samples. Mostly used in a research setting, it is now increasingly being used in the clinical realm as well, notably to support diagnosis of viral infections, thereby calling for quality control and the implementation of ring trials (RT) to benchmark pipelines and ensure comparable results. The Swiss NGS clinical virology community therefore decided to conduct a RT in 2018, in order to benchmark current metagenomic workflows used at Swiss clinical virology laboratories, and thereby contribute to the definition of common best practices. The RT consisted of two parts (increments), in order to disentangle the variability arising from the experimental compared to the bioinformatics parts of the laboratory pipeline. In addition, the RT was also designed to assess the impact of databases compared to bioinformatics algorithms on the final results, by asking participants to perform the bioinformatics analysis with a common database, in addition to using their own in-house database. Five laboratories participated in the RT (seven pipelines were tested). We observed that the algorithms had a stronger impact on the overall performance than the choice of the reference database. Our results also suggest that differences in sample preparation can lead to significant differences in the performance, and that laboratories should aim for at least 5–10 Mio reads per sample and use depth of coverage in addition to other interpretation metrics such as the percent of coverage. Performance was generally lower when increasing the number of viruses per sample. The lessons learned from this pilot study will be useful for the development of larger-scale RTs to serve as regular quality control tests for laboratories performing NGS analyses of viruses in a clinical setting.
Calves Infected with Virulent and Attenuated Mycoplasma bovis Strains Have Upregulated Th17 Inflammatory and Th1 Protective Responses, Respectively
Authors: Chao Jin , Han Xiaoxiao , Liu Kai , Li Qingni , Peng Qingjie , Lu Siyi , Zhao Gang , Zhu Xifang , Hu Guyue , Dong Yaqi , Hu Changmin , Chen Yingyu , Chen Jianguo , Khan Farhan Anwar , Chen Huanchun , Guo Aizhen
Published: August 2019

Mycoplasma bovis is a critical bovine pathogen, but its pathogenesis remains poorly understood. Here, the virulent HB0801 (P1) and attenuated HB0801-P150 (P150) strains of M. bovis were used to explore the potential pathogenesis and effect of induced immunity from calves’ differential transcriptomes post infection. Nine one-month-old male calves were infected with P1, P150, or mock-infected with medium and euthanized at 60 days post-infection. Calves in P1 group exhibited other clinical signs and pathological changes compared to the other two groups. Transcriptome profiles of peripheral blood mononuclear cells revealed seven and 10 hub differentially expressed genes (DEGs) in P1 and P150 groups compared with mock-infected group, respectively. Then, P1-induced pathogenesis was predicted to be associated with enhanced Th17, and P150-induced immunity with Th1 response and expression of ubiquitination-associated enzymes. Association analysis showed that 14 and 11 DEGs were positively and negatively correlated with pathological changes, respectively. Furthermore, up-regulated expression in molecules critical to differentiation of pathogenic Th17 cells in lung and peripheral blood mononuclear cells in P1 group was validated at RNA and protein levels. The results confirmed virulent and attenuated strains might be associated with biased differentiation of pro-inflammatory pathogenic Th17 and Th1 subsets respectively.
Rock Glacier Kinematics in the Kaunertal, Ötztal Alps, Austria
Authors: Groh Till , Blöthe Jan Henrik
Published: August 2019

The quantification of rock glacier kinematics on a regional basis has gained increasing importance in recent years. Here, we applied an image tracking approach on high-resolution aerial imagery to infer surface kinematics of 129 mapped rock glaciers in the Kaunertal, Austrian Alps. We find significant surface movement for 30 features with mean velocities falling between 0.11 and 0.29 m yr−1 and a maximum of 1.7 m yr−1. Local analysis and comparison to earlier studies reveals significant increases in rock glacier velocities in the study area. From the rock glacier inventory and high-resolution digital topography, we computed a series of morphometric parameters to analyze potential controls on rock glacier creep and to predict rock glacier activity using random forests and logistic regression models. The results point towards a stronger dependence of velocities on parameters describing general inclination, potentially acting as proxies for internal rock glacier properties, while activity states seem to be regulated mainly by rock glacier dimensions and topoclimate. Using a parameter subset, we successfully separated active from inactive rock glaciers with accuracies of up to 77.5%, indicating a promising approach to predict rock glacier activity solely relying on parameters that can be derived from regionally available data sets.
Spatial and Temporal Variability of Glacier Surface Velocities and Outlet Areas on James Ross Island, Northern Antarctic Peninsula
Authors: Lippl Stefan , Friedl Peter , Kittel Christoph , Marinsek Sebastián , Seehaus Thorsten C. , Braun Matthias H.
Published: August 2019

The northern Antarctic Peninsula was affected by a significant warming over the second half of the 20th century and the collapse of several ice shelves. Local climate conditions on James Ross Island on the northeastern coast can differ strongly from the main part of the Antarctic Peninsula. This paper reports the spatial and temporal variability of glacier surface velocities and the area of their outlets throughout James Ross Island, and evaluates potential relationships with atmospheric and oceanic conditions. Velocity estimates were retrieved from intensity feature tracking of scenes from satellite synthetic aperture radar sensors TerraSAR-X and TanDEM-X between 2014 and 2018, which were validated against ground observations. Calving front positions back to 1945 were used to calculate outlet area changes for the glaciers by using a common-box approach. The annual recession rates of almost all investigated glacier calving fronts decelerated for the time periods 2009–2014 and 2014–2018 in comparison to the period 1988–2009, but their velocity patterns differed. Analysis of atmospheric conditions failed to explain the different patterns in velocity and area changes. We suggest a strong influence from local bathymetric conditions. Future investigations of the oceanic conditions would be necessary for a profound understanding of the super-position of different influencing factors.
Nostalgia Makes Us All Tick: A Special Issue on Contemporary Nostalgia
Authors: Salmose Niklas
Published: August 2019

Nostalgia makes us all tick: It engages [...]
Multi-Scale Investigation of Body-Glaze Interface in Ancient Ceramics
Authors: Godet Marie , Roisine Gauthier , Beauvoit Emmie , Caurant Daniel , Majérus Odile , Menguy Nicolas , Dargaud Olivier , Bouquillon Anne , Cormier Laurent
Published: August 2019

Bernard Palissy is a French Renaissance ceramist renowned for his masterpieces called Rustiques Figulines on which dozens of glazes of different chemistries (and thus firing behaviors) coexist harmoniously. This study aims at gathering information on the master procedure -never revealed- by investigating the body-glaze interface region (focusing on iron-colored honey transparent glaze-white body system). Optical and electron microscopies including transmission electron microscopy (TEM) are used to characterize the micro and nanostructure of both archaeological and replicas interfaces elaborated in controlled conditions (firing time, cooling rate, addition of Al in the glazing mixture). Both types of interfaces are comparable: a modified paste area from which are growing a relatively continuous layer of interfacial crystals identified as lead feldspars (K,Ca)PbAl2Si2O8 micro-sized single-crystals incorporating mullite 3Al2O3.2SiO2 nano-sized single-crystals. Modification of the firing parameters and removal of Al from the glazing mixture change essentially the interface extension and the micro-crystals morphology. By comparing archaeological and replica interfaces and considering previous studies, we can now state that Palissy was very likely adding clay (Al) in his frit. Moreover, he was probably working with a firing time of more than 1 h followed by slow cooling in the oven.
Physical Activity and Sedentary Behavior Assessment: A Laboratory-Based Evaluation of Agreement between Commonly Used ActiGraph and Omron Accelerometers
Authors: Yano Shohei , Koohsari Mohammad Javad , Shibata Ai , Ishii Kaori , Frehlich Levi , McCormack Gavin R. , Oka Koichiro
Published: August 2019

Different models of accelerometer have the potential to provide a different estimate of the same physical activity or sedentary behavior. Our study compared the outputs of the Active Style Pro (ASP) and ActiGraph (AG) devices in assessing predicted metabolic equivalents (METs) for specific activities under laboratory conditions. Thirty healthy young adults wore two hip accelerometers (ASP and AG), simultaneously while performing twenty-two activities (eight sedentary, eight household, and six ambulatory activities) in a controlled laboratory setting. For the AG, predicted METs for each activity was calculated using four equations based on vertical-axis and vector magnitude data. Separate paired t-tests and Bland–Altman analysis examined the difference and agreement in METs between AG using four commonly used equations and ASP measurements for each activity. AG devices using different equations calculated significantly different outcomes for most activities compared with ASP devices. The smallest differences in predicted METs estimates between ASP and AG were observed for ambulatory activities. Ambulatory activities demonstrated the best agreement between ASP and AG regardless of which AG equation was used. Our findings can be used to assist researchers in their selection of accelerometer and output estimation equations for measuring physical activity and sedentary behavior in adults.
Social Inequalities in Exposure to Ambient Air Pollution: A Systematic Review in the WHO European Region
Authors: Fairburn Jonathan , Schüle Steffen Andreas , Dreger Stefanie , Karla Hilz Lisa , Bolte Gabriele
Published: August 2019

Ambient air pollution is a long-standing and significant public health issue. The aim of this review is to systematically examine the peer-reviewed evidence on social inequalities and ambient air pollution in the World Health Organization European Region. Articles published between 2010 and 2017 were analyzed in the review. In total 31 articles were included in the review. There is good evidence from ecological studies that higher deprivation indices and low economic position are usually linked with higher levels of pollutants such as particulate matter (particulate matter under 2.5 and 10 microns in diameter, PM2.5, PM10) and oxides of nitrogen (e.g., NO2, and NOx). There is also evidence that ethnic minorities experience a mixed exposure in comparison to the majority population being sometimes higher and sometimes lower depending on the ethnic minority under consideration. The studies using data at the individual level in this review are mainly focused on pregnant women or new mothers, in these studies deprivation and ethnicity are more likely to be linked to higher exposures of poor air quality. Therefore, there is evidence in this review that the burden of higher pollutants falls disproportionally on different social groups.
Health Professionals’ Knowledge of Probiotics: An International Survey
Authors: Fijan Sabina , Frauwallner Anita , Varga László , Langerholc Tomaž , Rogelj Irena , Lorber Mateja , Lewis Peter , Povalej Bržan Petra
Published: August 2019

The objective of this study was to survey health professionals to investigate their knowledge of probiotics. An online survey was conducted to gather data on the knowledge of health professionals. The online survey was distributed via email and social media platforms using snowball sampling. A total of 1066 health professionals (859; 80.6% female) from 30 countries responded to the survey. Most of the respondents evaluated their knowledge of probiotics as medium (36.4%) or good (36.2%). Only 8.9% of the respondents rated it as excellent. No statistical difference in knowledge was found between male and female health professionals. Over 80% of pharmacists, allied health professionals, medical doctors and dentists, and other health professionals knew the correct definition of probiotics as “live microorganisms that, when administered in adequate amounts, confer a health benefit on the host”, whereas three quarters of registered nurses and midwives and less than two thirds of psychologists identified the correct definition. Statistically, more female than male health professionals knew the correct definition of probiotics. The most frequently recognized species of bacteria containing probiotic strains were Lactobacillus acidophilus (92%), Bifidobacterium bifidum (82%), and Lactobacillus rhamnosus (62%). The opinions on when it is best to take probiotics were different (χ2 = 28.375; p < 0.001), with 90.2% of respondents identifying that probiotics have beneficial effects if taken during antibiotic therapy, 83.5% for diarrhea, 70.6% for constipation, 63.3% before traveling abroad, and 60.4% for treating allergies. Almost 79% of health professionals involved in this study have advised their patients to use probiotics and 57.5% of the respondents wanted to learn more about probiotics. All things considered, health professionals have a medium level of knowledge of probiotics, which could be improved by the implementation of targeted learning programs. As probiotics have many beneficial effects in a wide range of health areas, health professionals need to adopt the use of probiotics in clinical practice.
Chemical Effect of Bisphenol A on Non-Alcoholic Fatty Liver Disease
Authors: Dallio Marcello , Diano Nadia , Masarone Mario , Gravina Antonietta Gerarda , Patanè Vittorio , Romeo Mario , Di Sarno Rosa , Errico Sonia , Nicolucci Carla , Abenavoli Ludovico , Scarpellini Emidio , Boccuto Luigi , Persico Marcello , Loguercio Carmelina , Federico Alessandro
Published: August 2019

Non-alcoholic fatty liver disease (NAFLD) is considered a predominant chronic liver disease worldwide and a component of metabolic syndrome. Due to its relationship with multiple organs, it is extremely complex to precisely define its pathogenesis as well as to set appropriate therapeutic and preventive strategies. Endocrine disruptors (EDCs) in general, and bisphenol A (BPA) in particular, are a heterogeneous group of substances, largely distributed in daily use items, able to interfere with the normal signaling of several hormones that seem to be related to type 2 diabetes mellitus (T2DM), obesity, and other metabolic disorders. It is reasonable to hypothesize a BPA involvement in the pathogenesis and evolution of NAFLD. However, its mechanisms of action as well as its burden in the vicious circle that connects obesity, T2DM, metabolic syndrome, and NAFLD still remain to be completely defined. In this review we analyzed the scientific evidence on this promising research area, in order to provide an overview of the harmful effects linked to the exposure to EDCs as well as to frame the role that BPA would have in all phases of NAFLD evolution.
Walkability, Overweight, and Obesity in Adults: A Systematic Review of Observational Studies
Authors: Paulo dos Anjos Souza Barbosa João , Henrique Guerra Paulo , de Oliveira Santos Crislaine , de Oliveira Barbosa Nunes Ana Paula , Turrell Gavin , Antonio Florindo Alex
Published: August 2019

We conducted a systematic review to describe and summarize possible associations between the walkability index, overweight, and obesity. Systematic searches using seven electronic databases and reference lists were conducted to identify papers published until December 2017. Observational studies, describing associations using regression-based statistical methods, published in English and Portuguese, reporting markers of overweight and obesity, and involving adults (≥18 years) were included. Of the 2469 references initially retrieved, ten were used for the descriptive synthesis. Seven studies showed significant inverse associations between walkability and overweight and obesity, however, all were cross-sectional studies. High risk of bias scores were observed in “selection bias” and “withdrawals and dropouts”. All studies were published in high-income countries with sample sizes ranging among 75 to 649,513 participants. Weight and height as measures for determining BMI tended to be self-reported. Indicators of walkability, such as land-use mix, street connectivity and residential density were used as components of the indices. Based on this review, more studies should be conducted in low, middle, and middle-high income countries, using longitudinal designs that control neighborhood self-selection; other indicators of the neighborhood environment, such as food access, physical activity facilities, sidewalks, and safety and crime prevention should be considered.