bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancement of the Mechanical and Biological Properties of a Biomembrane for Tissue Engineering the Ocular Surface
Authors: Zhu Xiao , Beuerman Roger W , Chan-Park Mary BE , Cheng Zhiyuan , Ang Leonard PK , Tan Donald TH
Published: February 2023

Introduction: In this study, we have developed and optimised a novel gelatin-chitosan (GC) substrate for use as a cellular carrier for tissue-engineered conjunctival epithelium.

Materials and Methods: The substrate was fabricated by casting and the mechanical properties of the substrate, including tensile strength and elongation, were measured. Using the MTT, cell proliferation assay with rabbit conjunctival fibroblasts, we optimised the G:C ratio to enhance cytocompatibility. Rabbit conjunctival epithelial cells were immunostained using monoclonal antibodies for keratin 4 and pancytokeratin to investigate the biological effects of the GC substrate on the proliferation and differentiation of epithelial cells.
Results: We found that increasing the amount of gelatin resulted in an increase in elasticity (from 1:9 to 1:1 ratio), reaching a maximum (101.89% ± 7.13%) at a ratio of 1:1. The MTT assay showed that the proliferation of conjunctival fibroblasts significantly increased from 0.068 ± 0.017 to 0.177 ± 0.011 (P = 0.014) as the gelatin was increased from 20% (1:4) to 50% (1:1). Additional studies using tissue-cultured conjunctiva explants showed that these explants grew well on the substrate, forming a multilayered epithelium. Cell morphology on this substrate was similar to that of cells grown on culture dishes alone. Positive staining of keratin 4 and pancytokeratin indicated that the substrate supported normal differentiation of conjunctival epithelial cells.
Conclusion: By enhancing the proportion of gelatin, both the mechanical and biological properties of the chitosan substrate were improved. The results also suggest that this GC biomembrane may be a useful candidate for reconstructive tissue engineering of the conjunctiva.
ProgramaciÃ³n verde en el software
Authors: Barzola Edison
Published: February 2023

La TecnologÃ­a Verde se describe al uso eficaz de los recursos computacionales empequeÃ±eciendo el daÃ±o ambiental, extendiendo su posibilidad econÃ³mica y afirmando necesidades sociales. Esta de tal manera tan bien se puede definir como un conjunto de mÃ©todos que reducen el impacto informÃ¡tico sobre el medio ambiente, de tal manera que se considere el reciclaje de los tantos componentes utilizados para asÃ­ darle a otras personas la posibilidad de que continÃºen obteniendo el provecho de esta tecnologÃ­a. Esta no sÃ³lo identifica a las principales consumidoras de energÃ­a y fabricantes de desperdicios ambientales, sino que esta le ofrece el desarrollo de productos informÃ¡ticos ecolÃ³gicos y de tal manera promueve el reciclaje computacional. En cuanto al software verde, en 1992, la Agencia de ProtecciÃ³n Ambiental de EE.UU. puso en camino Energy Star, un programa de etiquetado voluntario para originar y registrar la eficacia energÃ©tica en los ordenadores, unidades de vigilancia climÃ¡tica, y otros mÃ©todos tecnolÃ³gicos.  En cuanto a Algunas de las tecnologÃ­as catalogadas como verdes debido a que apoyan a la reducciÃ³n en el consumo de energÃ­a o emisiÃ³n de diÃ³xido de carbono son computaciÃ³n en nube, computaciÃ³n grid, virtualizaciÃ³n en centros de datos y teletrabajo. La existencia del software verde depende, claramente, de donde arranca la energÃ­a utilizada en los equipos donde el mismo se desarrolla y usa. Esto se trata de un Ã¡rea en la cual no siempre es fÃ¡cil trabajar, primor dial mente por el impacto de los ordenadores y los servidores en los puntos del medio ambiente para la alimentaciÃ³n de los equipos.
As Serine/Arginine Protein Kinases De Leishmania braziliensis (LbSRPK’s) como novo alvo terapêutico para tratamento da leishmaniose tegumentar
Authors:   , Leopoldo Juliana Rodrigues
Published: February 2023

A Leishmaniose é uma doença infecto-parasitária causada por protozoários da espécie Leishmania. Segundo a Organização Mundial da Saúde, as leishmanioses são endêmicas em 98 países ao redor do mundo, que atinge principalmente países emergentes e em desenvolvimento localizada entre os trópicos. As leishmanioses são classificadas de acordo com os sintomas apresentados e podem se dividir em dois grandes grupos: as Leishmanioses Cutâneas e a Leishmaniose Visceral, a depender da espécie de leishmania que infecta o hospedeiro. No Brasil, os tratamentos de primeira escolha são os antimoniais pentavalentes e os de segunda escolha a anfotericina B em sua formulação lipossomal e quando esses medicamentos não podem ser prescritos, são usadas as pentamidinas. Todos esses medicamentos são disponibilizados pelos Sistema Único de Saúde (SUS) no Brasil. O tratamento muitas vezes necessita de vigilância hospitalar para monitoramento do paciente enquanto o recebe por ser muito tóxico e poder causar danos ao paciente. Isso gera efeitos citotóxicos muito graves e podem fazer com que o tratamento seja abandonado por parte do paciente que, na maioria das vezes, ou reside em local distante do estabelecimento de saúde, ou apresenta quadros de toxicidade aguda devido ao tratamento. Devido a isso, novas terapias leishmanicidas são necessárias de modo urgente, tanto no Brasil quanto no mundo. Já foi descrito por muitos estudos que as Serine Arginine Protein Kinases, as SRPKs, estão desreguladas em processos infecciosos como o HPV, HIV e doenças crônicas como o Alzheimer e o câncer. Além disso, um grupo de pesquisadores desenvolveu um inibidor de SRPK em humanos, o SRPIN340, que quando testado para leishmanioses, não apresentou nenhuma eficácia. Assim, com o presente trabalho, testamos compostos análogos sintéticos do SRPIN340 para o tratamento da Leishmaniose Tegumentar Americana, causada por Leishmania braziliensis. No total 91 compostos, de três séries foram testados e no ensaio de infecção observamos que todas as séries tiveram resultados promissores quanto a potencial atividade leishmanicida. Novos estudos devem ser realizados a fim de que se obtenham maiores informações quanto ao potencial leishmanicida desses compostos. Palavras-chave: Leishmaniose Tegumentar Americana. Alvo farmacológico. Serine Arginine Protein Kinases (SRPK’s).
Penggunaan Pelantar Google Classroom dalam Pengajaran dan Pembelajaran di Rumah bagi Konteks Murid Sekolah Rendah
Authors: Che Muhammad Nor Ahmad Fikri , Mohd Matore Mohd Effendi @ Ewan
Published: February 2023

Penularan wabak Covid-19 menyebabkan pembelajaran dalam talian melalui pelantar Google Classroom mula giat dijalankan bagi memastikan pengajaran dan pembelajaran di rumah (PdPR) dapat dilaksanakan. Google Classroom mula menjadi keutamaan dan dilihat mesra pengguna dalam membantu mewujudkan pengajaran dan pembelajaran yang berkesan dan efektif. Namun begitu, hasil sorotan mendapati terdapat beberapa isu dan kekangan yang wujud seperti Google Classroom belum diteroka sepenuhnya, kurangnya pendedahan dan sokongan, kesediaan guru dan murid, capaian internet yang terhad serta kekangan alat peranti. Beberapa cadangan penyelesaian dikemukakan seperti membantu tahap kesediaan dengan latihan guru, meningkatkan kesedaran dan kesediaan ibu, bapa dan penjaga, menggalakkan kerjasama kerajaan dan sektor swasta dan memperbaiki kemudahan dan infrastruktur ICT. Implikasi kertas konsep ini adalah menambahbaik kualiti penyampaian pengajaran dan pembelajaran kepada pelajar Sekolah Rendah melalui Google Classroom. Kajian lanjutan juga boleh dikembangkan dengan meneroka peluang kolaborasi antara pihak sekolah bersama industri bertujuan meningkatkan penggunaan Google Classroom secara lebih efektif dan menyeluruh.
Kesahan Modul Pembangunan Produk bagi Subjek RBT Tingkatan 3 dalam kalangan Guru SMK di Selangor
Authors: Yusoff Nurul Najwa Binti , Che Kob Che Ghani Bin , Ismail Maswani
Published: February 2023

Kajian ini dilakukan untuk menguji kesahihan kandungan Modul Pembangunan Produk bagi mata pelajaran Reka Bentuk Dan Teknologi (RBT) Tingkatan 3 dalam kalangan guru Sekolah Menengah Kebangsaan (SMK) di Selangor. Kajian ini merupakan kajian kuantitatif yang menggunakan kaedah penyelidikan kajian tinjauan (survey) untuk mengumpul data melalui soal selidik yang berkaitan dengan penilaian keseluruhan terhadap kandungan modul yang dibangunkan. Sampel kajian terdiri dari tiga orang panel pakar yang telah ditetapkan ciri-cirinya. Ketiga-tiga panel pakar ini mestilah merupakan guru RBT yang mengajar di sekolah menengah harian. Selain itu, panel pakar juga haruslah mempunyai pengalaman mengajar dalam mata pelajaran Kemahiran Hidup Bersepadu (KHB) dan juga Reka Bentuk dan Teknologi (RBT) lebih daripada 10 tahun. Keputusan bagi kajian ini menunjukkan bahawa modul yang dibangunkan mempunyai kesahihan yang tinggi sebanyak 92 peratus. Dapatan kajian menunjukkan kesemua item di dalam konstruk modul Reka Bentuk Dan Teknologi seperti kulit hadapan modul, pengenalan, panduan penggunaan, objektif dan kandungan modul mempunyai kesahan kandungan yang tinggi berdasarkan pandangan dari perspektif pakar. Justeru, kesahan kandungan modul Reka Bentuk Dan Teknologi yang tinggi dapat menjadikan modul ini sebagai panduan dan rujukan oleh guru dan murid.
Revalidating a Writing Strategy Scale for Undergraduate Students: A Fuzzy Delphi Approach
Authors: Mohamed Hamdan Hanani , Samsuri Maimanah , Mat Pardi Khalid , Nordin Noli Maishara , Mazlan Siti Aisyah
Published: February 2023

The purpose of this study is to get expert consensus and comments on the Writing Strategy Scale for undergraduate students. The general consensus is that students who write in a language other than their mother tongue frequently struggle with coherence, consistency, syntax, and word choice. The Fuzzy Delphi approach is used in this study to collect information from 5 experts from Malaysian public institutions utilising a 7-expert scale. Experts are people who have put in the effort to earn their credentials, training, experience, professional membership, and peer recognition. The questionnaire consists of 29 items on the writing strategies used by undergraduate students. For data analysis, Fuzzy Delphi approach Logic Software (FUDELO) was employed. A triangular fuzzy number and a rating of the defuzzification procedure for each construct element were used to analyse the data. The findings of the study and expert consensus indicate that the Agreement's Value is adequate. This confirms that the Writing Strategy Scale items for undergraduate students were highly regarded by experts. The components that the experts agreed on in consensus are organized in order of importance. Thus, researchers were able to offer a fundamental framework for adopting writing strategies for learners in general and undergraduates specifically.
SMEs FINANCING – AN OVERVIEW OF DIRECT FINANCING ON CAPITAL MARKET VS. FINANCIAL INSTITUTIONS
Authors: BOZINTAN (COSMA-GULER) Ioana-Carmen
Published: February 2023

The importance of the role played by SMEs in the global economy and their contribution to job creation, added value, innovation and growth is widely acknowledged, therefore it is extremely necessary to support their development. In order to be competitive, SMEs also rely on external financing, not only through financial institutions but also through direct participation in the capital market. Managers place great emphasis on financing their businesses, and most of the European companies relying primarily on bank loans as their most important source of founding, unlike in the United States of America, where the activity of stock exchanges is much more developed. This paper aims to study a shared analysis of the advantages and disadvantages of SMEs in Romania regarding financing through financial institutions vs direct participation in the capital market.
WHO IS GOING TO WIN: THE EU ESG REGULATION OR THE REST OF THE WORLD? – A CRITICAL REVIEW
Authors: FRECAUTAN Ion , NITA (DANILA) Andreea
Published: February 2023

This paper aims to explore the past and the present challenges of EU ESG regulatory framework. Moreover, it attempts to make an in-depth analysis of the major ESG frameworks and standards used at a global level: GRI, SASB, IIRC, CDP, CDSB, TCFD, CSRD, and EU Taxonomy. The analysis considers 5 criteria: framework vs. standard, shareholder perspective, reporting format, metrics, and materiality. In addition, we draw insights into the complexity of the EU ESG reporting scheme and its added value compared to already existing frameworks and standards. Our results have implications on three levels: 1) for reporting companies that are faced with the option of selecting a reporting framework that will minimize the cost of compliance but also help them implement their climate transition strategy; 2) for employees and consumers of goods and services provided by companies that incorporate ESG in their long term economic activities and 3) policymakers that want to make sure they design the best standards that will ensure a smoother and effective transition to a low carbon economy.
AN OVERVIEW OF ROMANIAN ACCOUNTANTS REGARDING THE INTRODUCTION OF HUMAN CAPITAL IN FINANCIAL STATEMENTS
Authors: COCARIU Ana-Maria
Published: February 2023

Human capital is an indispensable element that strongly contributes to the success and performance of organizations, being considered the most valuable resource of companies. This study aims to bring to the fore the development of human capital and, at the same time, to identify the position of accounting experts regarding the quantification of human capital and how this measurement can contribute to obtaining a more accurate and faithful picture of the financial position of the company. Therefore, to achieve these objectives, a survey was created that was distributed to the members of the Body of Expert and Licensed Accountants of Romania (C.E.C.C.A.R.), thus obtaining a number of 139 responses. The results show that 122 of the respondents consider it necessary to quantify human capital to present the company’s image in a more accurate and realistic way. At the same time, 80 of these respondents do not consider that, once human capital is measured, it should be included in the financial statements, respectively in the balance sheet. This paper can provide an updated picture of the point of view of accounting professionals who have participated in the research on human capital, but also a starting point for the future steps of entrepreneurs in terms of human capital development, outlining new directions of development of professionals in the field. Also, this paper may be of interest to the institutions that regulate the relevant professions in the financial-accounting field, to outline new directions in the measurement and evaluation of human capital.
THE LATEST CRISES CHALLENGING THE EU`S EXPORTS. ANY CHANCES TO MAINTAIN THE COMPETITIVENESS?
Authors: MIERLUȚ Dana-Teodora , GIURGIU Adriana , MINTAȘ Horia-Octavian , TUNDUC Adrian
Published: February 2023

Trade is at the centre of Europe’s model of economic prosperity and competitiveness. As stated in the new Trade Policy Strategy set out by the European Commission (2021), the trade policy has a key role to play in the recovery from the COVID-19 pandemic. Meanwhile, this role was emphasised even better by the effects derived from the war in Ukraine. As the pandemic hit in 2020, the growth drivers of the EU’s economy relented, among which the external trade, putting million jobs at risk and leading to rise in unemployment and to depression of economic activity, while many companies had to close down. Competitiveness is a term which increased in significance in the last few decades. Trade competitiveness and, especially, export competitiveness is one of the most valuable variables a country must improve in order to increase its overall competitive position among nations and also the welfare of its citizens. The export statistics of many EU’s economies changed since the COVID-19 crisis and later, the war stroke. The research questions this paper addresses are: what is the extent of these changes in which sectors? Have they come to an end or the process of recovery has already started within the EU? What effects has the war in Ukraine onto the EU exports competitiveness? Are there any chances for the EU to maintain its exports competitiveness after these crises? What is to be one and what measures to be taken to recover? After introductory theoretical remarks and a brief literature review followed by a survey of various techniques to measure export competitiveness, the paper shifts its focus on statistical data and analyse how much damage has the pandemic and nowadays, the war caused to the EU’s export competitiveness, and which EU Member States were the most affected, based on statistical data analysis and other various indicators.
FOREIGN DIRECT INVESTMENT AND THE IMPACT ON EXPORTS AND GDP GROWTH. A BRIEF GLOBAL PERSPECTIVE
Authors: NEGREA Adrian , BENEA Ciprian Beniamin
Published: February 2023

People have been moving from one area to another since ancient times in order to exchange products from one nation with those from another in order to obtain what was lacking in the nation where they resided. These were the first product-to-product trades, and the latter was placed into circulation on money as a product-to-product trade. These early types of commerce have evolved significantly, and they continue to do so now as capital flows from one country to another. This phenomenon, which has influenced worldwide economic growth and improved living standards, saw significant expansion in the twenty-first century in the form of loans used to fund economic development in many nations, as well as ownership of financial assets. In the present era, domestic capital is insufficient for countries to thrive and remain competitive in global markets. For emerging economies, foreign investments that enter the host country as debt or money are crucial financial resources. In this paper, we will discuss the data on the development of exports and FDI of 22 countries from around the world and analysed the evolution of FDI, GDP and exports over a period of 40 years to see trends and patterns of FDI and Exports and their contribution to the economic growth of countries. Broken up in groups, the paper will compare and assess certain countries/regions, grouping and pairing them with economies or countries of similar growth/stance. Corroborating this statistics with recent global events and based on the previous statistics, this paper will try to predict the future trends of FDI around the world.
THE ECONOMIC FREEDOM, COUNTRY RISK AND FOREIGN DIRECT INVESTMENTS
Authors: ELENES PLATONA Iulia
Published: February 2023

The interlinkages between country risk and foreign direct investments are the subject of research interest. The article tests the intuitive hypothesis that economic freedom is associated with low country risk and is an incentive for foreign direct investments. The research paper employs empirical quantitative within-between models to analyze the relationship between foreign direct investments and five indices: trade openness, freedom from corruption, trade freedom, investment freedom, and economic freedom. The database used is The Global Economy for 44 European Countries resulting a panel data employed for between within models, growth curve models, contextual models, generalized estimating equations models (GEE), and asymmetric effects models. Interesting is the different significance of the five indicators in different models. For the first three models within -between model, the growth curve model and the contextual model- statistical significance have trade openness, freedom from corruption, and investment freedom. For the Generalized equations model (GEE) the only indicator that has statistical significance is Investment freedom. For the asymmetric effects model that shows the effect of asymmetric increase and decrease of each indicator, there is no statistical significance for the analyzed indicators. The within – between models combine the robustness of the fix effects models with the flexibility of the random-effects models.
FROM GENERAL TO PROFESSIONAL ENGLISH – CURRICULUM, STUDENTS, TRAINERS
Authors: SIM Monica-Ariana , POP Anamaria-Mirabela
Published: February 2023

The present study is an attempt to offer a bird’s eye view into the relationship between professional English, meaning English for Specific Purposes (ESP) and English for General Purposes (EGP) or 6General English (GE), stressing the difficulties that may appear on the road. There are opinion discrepancies regarding the differences that exist/do not exist between Business English teaching and General English teaching. Therefore, the general purposes and distinctive features are examined to draw a parallel between ESP and EGP to present their common and specific characteristics.
THE ROLE OF ARTIFICIAL INTELLIGENCE IN CONTENT CREATION AND CHECKING ITS EFFECTIVENESS IN THE GOOGLE ADS ADVERTISING SYSTEM
Authors: SOMOSI Zoltán
Published: February 2023

n Hungary, 94% of businesses have Internet access and 63% have a website. Moreover, online retail sales will reach HUF 1,203 billion in 2021. In order for companies to achieve the largest possible market share, they can use various digital marketing strategies. They are distinguished according to different methods. One of the most commonly used in practice and in science is inbound (as search engine optimization) and outbound (as advertising). Google Ads, which emerged at the turn of the millennium and defined itself as the world’s first company to use machine learning technology, is a market leader. Their ad system was initially based on keywords, which have since been expanded to include more than 4,800 types of targeting criteria. These targeting options are available for a variety of ad formats. The digital solutions to the billboards of traditional marketing are banner ads, called Display on Google. These ads contain image, video, and text content and aim to interrupt the consumer’s activity and redirect them to the advertiser’s website. Since they are capable of increasing website traffic by up to 300%, this can be interpreted as an opportunity that is also considered favorable by businesses. It is also suitable for testing various content elements, as one of its main indicators, the click-through rate, expresses the relevance of the ad, as several researchers have noted. As the role of artificial intelligence grows, more and more companies are using it as a competitive advantage. Some of their algorithms are capable of generating text, images, videos, or other content. In this study, I leverage the power of display ads and conduct my research in the Google Ads system instead of conducting consumer surveys. I created two ads for the same target audience, with the same budget and settings. The text content for one ad was created by a marketer, the image content was created by a professional photographer, and the content elements for the other ad were provided by Artificial Intelligence. The objective of the article is to study the performance, efficiency, and impact of artificial intelligence-generated content on conversions under real market conditions. The study also includes content created by the players.
ORGANIZATIONAL PERFORMANCE IS HIGHLY INFLUENCED BY MOTIVATION. A CASE STUDY OF THE PUBLIC HEALTH DIRECTORATE
Authors: ANGHEL Cristiana Maria , ALMASAN Alexandru Ionut
Published: February 2023

High productivity is of primary concern to individuals, the management of any organization and to the national economy at large. Performance management in the health sector is essential for access to health services and for the provision of quality services. It is generally regarded as a well-known fact that an organization’s employees are a key asset for success. One of the most fascinating questions for specialists, but also one that causes headaches for managers in organizations, is “why are people motivated to do something?”. A human’s ambitions and goals may differ from one person to another, so motivation is a crucial aspect of health care management. Management can guide people to achieve the organization’s goals by identifying their unique qualities and potentials. The main objective of the study was to analyze the factors that motivate and demotivate employees in the Public Health Directorate, in order to find, or demonstrate that workplace satisfaction is influenced by a combination of factors, each of them having their own effect, contributing to the final result, which can be decisive in creating or not an productive equilibrum within the organizations that were subjected to research. Research methods: qualitative-quantitative analysis through the questionnaire-based survey method. Research thesis: Motivation plays a crucial role in organizational performance. This article concluded that not only financial benefits are important to both male and female employees, but also non-financial benefits and Managers must attach significant importance to motivational factors because these are the only ones capable of guaranteeing the success of such an approach.
IMPACT OF WORKING FROM HOME ON PRODUCTIVITY & PERFORMANCE, EVIDENCE FROM NORTH AMERICAN LOGISTICS INDUSTRY DURING COVID-19 PANDEMIC
Authors: YEGUL Fatih , ACIKGOZ Atif , KAZIMOV Zaur
Published: February 2023

This paper analyzes the impact of shifting all employees from office-based working to working from home (WFH) on productivity and performance. Before the COVID-19 Pandemic, there were no instances of companies moving all of their employees to WFH indefinitely. This is one of the first case studies analyzing this new phenomenon supported by hard criteria for productivity and performance measures. We define productivity as the quantity and performance as the quality of the output. We collected our data from a North American 3rd Party Logistics company and employed a multimethod approach to examine the effects of this organizational change forced by the COVID-19 Pandemic. We gathered data from the employees through video interviews and online surveys. We compiled the performance metrics from the corporate database and received commentary from top management. We also used national statistics databases to delineate the specific market conditions. Figures indicate evident improvements in revenues, profits, and labor productivity after the shift to WFH. We also examined other potential moderating factors, such as changes in market conditions, improvements in business processes, and/or management approaches, to distinguish the effect of remote working. Overall results show that when accompanied by solid leadership strategies and proper communications, and with investment in IT technologies, working from home improves productivity with little or no effect on performance. Our findings offer valuable insights for managers who need to make strategic decisions about WFH arrangements for their companies, especially brokerage-type businesses, more specifically those in the logistics industry. The study is based on data from a case study involving one company, preventing the authors from generalizing their findings.
THE PRINCIPALS’ STRESS IN BEDOUIN SCHOOLS IN ISRAEL DURING CORONA PANDEMIC
Authors: ABURKAYEK SLEIMAN
Published: February 2023

The aim of this research is to explore the stress of the principals in Bedouin sector schools in Israel concerning remote teaching during COVID-19 pandemic. The interviews included eight (8) open–ended questions and were conducted with three (3) principals from different Bedouin schools in Negev concerning stress at work in the shadow of COVID-19. The main finding of this research is that the school principals in Bedouin were experiencing high levels of stress concerning distance teaching in the shadow of COVID-19 pandemic. The research findings also show that there is a lack of resources for remote learning among students in the Bedouin population, which was associated to the lack of the school principals’ control over teachers during the COVID-19, and was related to changes in the students’ behavior.
GENETIC DIVERSITY OF THE EAST EUROPEAN VOLE (MICROTUS ROSSIAEMERIDIONALIS) IN BELARUS
Authors: Mashkov E. I. , Gajduchenko H. S.
Published: February 2023

Data on the genetic variability of the mtCytb gene of the East European vole (Microtus rossiaemeridionalis) from Belarus are presented. In total, the haplotypes of 27 individuals of the East European vole from 8 countries were analyzed during the work. To verify the species belonging of the cryptic species Microtus arvalis s.l., a karyological analysis was also performed. The species composition of the cryptic group was determined by both genetic markers: Microtus arvalis "arvalis" (2n = 46, NF = 84) and Microtus rossiaemeridionalis (2n = 54, NF = 56). The East Eastern European vole inhabiting the territory of Belarus was confirmed to belong to the wave of colonization from South Asia along the northern corridor and possible re-settlement in this territory from the Ural refugium after the last glaciation (12 900–11 700 thousand years ago). The ways of further expansion of the species into the northern and southern parts of Europe were established.
DEVELOPMENT OF THE CRISPR/CAS9 SYSTEM FOR THE GENOME
EDITING OF THE NtPDS GENE OF TOBACCO (NICOTIANA TABACUM)
Authors: Shishlova-Sokolovskaya A. M. , Khmilevskaya E. P. , Urbanovich O. Yu.
Published: February 2023

The CRISPR/Cas9 system is one of the most powerful tools for the editing of plant genomes. In the presented study, the vector constructs developed on the basis of the CRISPR/Cas9 system were used to edit the Nicotiana tabacum genome. The NtPDS gene encoding the 15-cis-phytoene desaturase enzyme was chosen as the target. A knockout of this gene in plants results in the albinism phenotype and dwarfism. Using the in silico bioinformatics platforms, three vector constructs based on the binary pRGEB31 vector were modeled: pRGEB31 + gRNA4-pds, pRGEB31 + gRNAJp2-pds, and pRGEB31 + gRNADeT186-pds, carrying in its composition the CRISRP/Cas9 system with spacers to different parts of the structural domains of the NtPDS gene. Vector constructs were assembled using molecular cloning techniques. The accuracy and correctness of their assembly was confirmed by Sanger sequencing. Вy means of Agrobacteriummediated transformation of leaf discs, the genetic constructs were introduced into the genome of the N. tabacum cv.

Petit Havana SR1 model object. During the cultivation of tobacco leaf discs, it was possible to initiate callusogenesis and morphogenesis processes using all three constructs, however, the maximum frequency of these processes was observed when using the pRGEB31 + gRNA4-pds construct.
Foreign and national experience in a predicate crime setting
Authors: Kudratillayev Khazratali
Published: February 2023

This article analyzed issues related to the identification and definition of predicate crimes, which are considered as the main crime in the commission of crimes related to the legalization of proceeds from criminal activity. Also, the article analyzes the international conventions that should be taken into account by the countries when defining the predicate crime, and the definition that is suitable for the legislation of Uzbekistan was developed.
Cyberculture networks in the literature from 2019 to 2022
Authors: Morales Francisco Espinoza , García Lirios Cruz
Published: February 2023

Gross Similarly , cyberculture refers to a scenario of intellectual capital, which is an intangible asset for organizations focused on the management, production and transfer of knowledge. In this sense, the usefulness and ease of use of information technologies presuppose computational skills, but these entail risks and stigmas that inhibit the climate of relationships, support and innovation. Therefore, the establishment of a model for the study of organizational cyberculture around knowledge innovation is the objective of this work. A documentary study was carried out with a selection of sources indexed to national repositories in order to make a model for the study of cyberculture more complex. Lines of research concerning the amplification of repositories, techniques and theories around the amplification of the model are noted.
Practical Exploration of Integrating Civics into Professional Courses in Open Education Courses
Authors: Zhang Lili
Published: February 2023

Open education is a kind of education mode relative to traditional education and closed education, featuring student-oriented, diversified teaching methods, no learning restrictions and a high degree of student autonomy. In order to better implement the fundamental educational goal of establishing moral education in the national open university, curriculum thinking politics has become an inevitable trend of curriculum reform in colleges and universities, and an effective means to improve the overall quality level of contemporary talents, which is like a link between thinking politics courses and non-thinking politics courses, and can promote the thinking politics of professional courses with the characteristics of open education, so as to improve the overall quality  level  of  students  and  promote  their  overall  development.  This  paper  introduces  the  role  of  open education courses as a link between open education courses and non-catholic courses, so as to improve the overall quality of students and promote their overall development. This paper introduces the significance of integrating the open education curriculum into the professional courses, and analyzes the current situation of the open education curriculum and the practical methods of its integration into the professional courses.
Innovative Reform of "Curriculum Civics" in Music Performance Professional Skills Course
Authors: Li Wenliang , Liu Wenzong
Published: February 2023

For colleges and universities, music performance is one of the important disciplines in their art disciplines. When teachers teach the skills courses, they should incorporate the elements of thinking and politics into them, innovate the existing teaching methods, constantly update the teaching contents, strengthen the students' "thinking and politics" consciousness in their eyes and ears, enhance their national self-confidence, improve the practical significance of modern In this paper, we discuss the practical significance of social science and political education and promote students' all-round development. In this paper, the importance of "Civic Science and Politics" in music performance courses is explored, and specific innovative reform paths are proposed.
The integration of electrical and mechanical education and innovation and entrepreneurship education
Authors: Wu Xiayi , Han Yangqi
Published: February 2023

At present, China is in a period of modernization and development, and innovation and entrepreneurship have become the main trend of social development under the new situation of "mass entrepreneurship". At present, many institutions of higher education have taken innovation and entrepreneurship education as an important educational strategy for their own development, wanting to give their students a greater competitive advantage in the increasingly competitive market. Therefore, the integration of innovation and entrepreneurship education and electromechanical education is not only the inevitable trend of educational reform and development of higher education institutions in the new era, but also the inevitable result of the demand of society for modern and innovative talents, and the combination of the two is not only beneficial to enhance students' competitiveness in society but also can effectively implement students' employment quality.
Punctate Inner Choroidopathy in a Young Filipino Female: A Case Report
Authors: Santos Edmin Michael G.
Published: February 2023

A 30-year-old, monocular myopic female consulted for new onset scotoma on her left eye. Best corrected visualacuity was 20/20 for the left eye. Fundus exam revealed a hypopigmented lesion in the inferior juxtafoveal areawith no overlying vitreous reaction/opacity. Spectral domain optical coherence tomography (SD-OCT) showedfocal disruption and elevation of the inner choroid and retinal pigment epithelium (RPE), indicating punctate inner choroidopathy (PIC). OCT angiography (OCT-A) revealed the presence of a choroidal neovascular membrane (CNV) on RPE-RPE fit segmentation. The CNV and lesion resolved upon treatment with one intravitreal bevacizumab injection and oral prednisone 60 mg (1.2 mg/kg body weight) per day tapering over 2 months.This is the first reported case of PIC in a Filipino that was diagnosed and treated with the guidance of high-resolution SD-OCT and OCT-A. These diagnostic tests proved useful in identifying the lesions based on cross-sectional views of the retina, RPE and choroid.
A Techno-Economic Analysis of Utilization and Development Activated Carbon as Biomass-based Electrodes for Supercapacitor Device
Authors: Taslim Rika , Pahlevi Farhan Dio , Suedi Suedi , Apriwandi Apriwandi , Harpito Harpito , Hamdy Muhammad Ihsan , Kusumanto Ismu , Siska Merry , Taer Erman
Published: February 2023

Biomass-based activated carbon (AC) has been widely used as a supercapacitor electrode. Although there have been many studies that explain the potential of biomass as a material to produce AC as supercapacitor electrodes in performance through specific capacitance values and energy values, there have been no studies that discuss their potential from an economic point of view. Therefore, this study aims to use mission grass as a supercapacitor electrode to produce AC based on techno-economic aspects. There are several calculations, including the cost of production, break-even point (BEP), payback period (PP), net present value (NPV), and sensitivity analysis. Furthermore, it is necessary to include an estimate of cost component data to calculate the small-scale production target of 5,472 units/year. The results showed that cost of production of USD 1.83/unit with a selling price of USD 2.37, BEP at 3,538 units earned a gross profit, PP, and a positive NPV of USD 8,380, 5 years, and USD 8,768, respectively. According to sensitivity analysis, changes in selling price to BEP are considered the most sensitive. These implies that using mission grass as a supercapacitor electrode is beneficial.
HIGHLIGHTS

Development mission grass as a supercapacitor electrode is beneficial
Produce activated carbon in small-scale production up to 5,472 units/year
The payback period obtained in 5 years, NPV positive of USD 8,768 and earned gross profit of USD 8,380
Selling price of the supercapacitor biomass-based cheaper than other

GRAPHICAL ABSTRACT
The effect of complex application of symbiotic and biocidal preparations on the metabolic status of broiler chickens' blood
Authors: Chechet Olha , Shulyak Svitlana , Kovalenko Vyacheslav , Romanko Maryna , Haidei Olha
Published: February 2023

Using probiotic preparations and their complexes in poultry farming is an important step towards increasing poultry resistance and productivity, and thus producing environmentally friendly products. The purpose of the study was to investigate the effect of the complex action of symbiotic drugs and biocidal agents by investigating the clinical and biochemical parameters of blood and mineral metabolism in the body of broiler chickens under the conditions of their use according to the developed scheme. Hematologic, biochemical and immunologic blood parameters were explored by conventional methods, micro- and macroelements by optical emission spectrometry with inductively coupled plasma. Using the proposed complex of symbiotic and biocidal agents in the technology of rearing broiler chickens activates the processes of erythropoiesis, haemoglobin production, restoration of protein metabolism and normalisation of mineral status within physiological limits, which indicates the strengthening of the body's defences, increased resistance to stress factors and adverse environmental conditions, and will further have a positive effect on the growth and productivity of the target bird. The pronounced effect of the complex combination of drugs on the body of broiler chickens of experimental B is a better assimilation of many inorganic elements (by increasing the content of Calcium, Phosphorus, Cuprum, Ferrum and Selenium, Magnesium, Manganum); and, accordingly, causes the sustainable development of metabolic processes due to the prevalence of anabolic over catabolic processes (by increasing the level of total proteins against the background of a physiological increase in the number of globulin fractions; including the induction of the endogenous detoxification system – by reducing the level of production of toxic metabolites of purine metabolism: uric acid and creatinine; reduction of Chromium content; normalisation of transamination processes and deceleration of alkaline phosphatase activity) and immunobiological reactions (by enhancing erythropoiesis and haemoglobin production; induction of lysozyme, bactericidal and phagocytic activity), respectively. The proposed system of rearing young poultry using a complex of symbiotic preparations in combination with biocidal agents promotes the synergistic effect of the latter and their effective action on the body of experimental broiler chickens, which increases nonspecific resistance and restores metabolic reactions
A EXTRAJUDICIALIZAÇÃO NO DIREITO BRASILEIRO: CONFLITOS DE INTERESSE ENTRE CARTÓRIO E A SOCIEDADE – A REALIDADE DO USUCAPIÃO (ADMINISTRATIVO)
Authors: Silva Sidney , Cunha Eduardo
Published: February 2023

A extrajudicialização no direito brasileiro aborda os conflitos de interesse entre cartório e a sociedade. Um conflito no dia a dia pode ser resolvido na prática com a ajuda do cartório, podendo ser peça chave na desjudicialização, levando ao conhecimento da sociedade todas as ferramentas necessárias ao extrajudicial. Pode ser utilizada no litígio comum no meio social atual e deve estar presente para levar celeridade e segurança jurídica para aqueles que necessitam. Cabe ao poder público, em geral, fazer com que este tema faça valer sua perspectiva na sociedade, firmando parcerias com as entidades privadas, pois fica claro que a sociedade não está aquém do direito sobre sua inclusão junto ao extrajudicial. O que se pode ver é que quanto mais direito para a sociedade, mais ela se afasta por não ter conhecimento ou falta de divulgação, tendo a dignidade de ter um simples imóvel registrado em cartório competente, sendo tarefa difícil no Brasil, mesmo com normas objetivas para o cidadão. Palavras-chave: Extrajudicialização no direito brasileiro. Cartório e sociedade. Conflitos de interesses. Usucapião extrajudicial.
CÓDIGO PENAL BRASILEIRO: DESAFIOS DE APLICABILIDADE NO CONTEXTO MORAL SOCIAL
Authors: Andrade Roberto , Silva Jonas
Published: February 2023

Este trabalho aborda os desafios da aplicabilidade do Código Penal Brasileiro ante à conduta moral da sociedade em constante transformação ao longo do tempo. O Código Penal Brasileiro é considerado de suma magnitude, posto ser ainda hoje recorrente uma variedade de pensamentos discordantes, ao ponto de muitos que se enveredam pelo ramo jurídico não chegarem a um consenso quanto às interpretações do referido Código em relação ao tratamento da concepção do que é “moralidade”. Palavras-chave: Código Penal. Contexto moral e social. Desafios na aplicação do CP.
LIDERANÇA FEMININA: DESAFIOS DA MULHER NO MERCADO DE TRABALHO
Authors: Campelo Arandi , Paz Carolaine , Santos Lydiane
Published: February 2023

Este artigo trata da liderança feminina e os desafios que a mulher enfrenta no mercado. O estudo teve como objetivo compreender as principais características de mulheres líderes e as condutas no mercado de trabalho, identificando as estratégias, pilares, e os métodos da liderança feminina para alcançar a eficácia em suas ações. Este trabalho foi desenvolvido através de pesquisa bibliográfica e de campo. Os principais teóricos que demarcaram este artigo foram Nascimento (2018), Ana Bernal (2021) e Tonani (2011). Neste estudo qualitativo, foi utilizado como principal ferramenta para coleta das informações uma entrevista semiestruturada, tendo como campo investigativo mulheres líderes da empresa Delikata, uma doceria com sede em Olinda-PE e lojas espalhadas por bairros do Recife e Jaboatão dos Guararapes. Da totalidade de 6 líderes, 3 participaram das entrevistas, no ano de 2022. Com base nos dados coletados em campo foi possível concluir sobre a grande relevância que as mulheres e suas competências representam, no contexto do ambiente profissional, notadamente no papel de liderança eficaz para as organizações. Palavras-chave: Liderança feminina. A mulher no mercado de trabalho. Síndrome de Burnout.
Evolución formal y constructiva de las escaleras de caracol en la Catedral de Sevilla (1433-1560)
Authors: Pinto Puerto Francisco
Published: February 2023

Las escaleras de caracol asumieron un papel fundamental en el proceso constructivo de la catedral hispalense y una vez alcanzada la altura de las terrazas, tras cerrar las bóvedas y cubiertas, adoptaron un papel relevante en la apariencia exterior del edificio, emulando pequeños chapiteles, linternas, pináculos y cúpulas. Un repertorio formal en el que podemos percibir la evolución de estos elementos arquitectónicos. Planteamos en este artículo un análisis formal y constructivo de estas escaleras, siguiendo la peculiar manera en la que se levantó esta fábrica gótica, desde los pies a la cabecera, y como se introducen nuevos modelos en la etapa renacentista. Identificaremos sus variantes tipológicas, su capacidad para constituirse en elementos funcionales, representar significados, así como constituirse en eficaces indicadores cronológicos.
La carpintería mal llamada Mudéjar
Authors: Nuere Matauco Enrique
Published: February 2023

En el último tercio del siglo XX la carpintería de lazo realizada en la totalidad del territorio español estaba escasamente estudiada lo que hizo que se asumiera su origen como mudéjar. Una investigación a fondo sobre este arte peculiar prueba que su origen se basó en técnicas visigodas y que la convivencia con un nuevo cliente islámico pudo influir en su creación. Sin embargo siempre estuvo más enraizada con el mundo cristiano que con el islámico, hasta el punto que prosperó juntamente con el desarrollo del arte gótico y finalmente quedó consolidada con la dinastía Trastámara.
Use of Slack for COVID-19 disaster response in Hyogo Prefecture
Authors: Kuwahara, MD Masaatsu , Kawase, MD, PhD Tetsunori , Kai, MD Soichiro , Shimadzu, MD, PhD Kazuhisa , Ishihara, MD, PhD Satoshi , Hirata, MD, PhD Jun-ichi , Nakayama, MD, PhD Shinichi
Published: February 2023

Introduction: The coronavirus disease 2019 (COVID-19) pandemic has caused great disruptions in education and healthcare systems. However, before the COVID-19 pandemic, Hyogo Prefecture did not have a centralized system for sharing information among hospitals, public healthcare centers, the government, fire departments, and medical associations. In March 2021, we used Slack® an enterprise social network, to invite people from hospitals, healthcare centers, the government, fire departments, and medical associations to join our workspace. This study was undertaken to verify whether the use of Slack can improve information sharing during a disaster.Methods: A questionnaire on whether information-sharing tools such as Slack are useful for disaster response in Hyogo Prefecture, including for COVID-19, was administered using a Google Form.Results: Of the 19 people who responded to the questionnaire, 15 (78.9 percent) were first-time users of Slack and 18 (94.7 percent) found it easy to use. All 19 participants stated that Slack proved useful in providing pertinent information regarding preventive measures for the control of COVID-19, and they believed that it would be a helpful tool if such disasters were to occur in the future. Regarding the launch time of Slack, 11 respondents (57.9 percent) answered that it was launched at the appropriate time, while five respondents (26.3 percent) answered that it was launched too late. Conclusions: The participants thought that Slack was effective for accelerating their communication. The use of Slack could also improve future disaster response communications.