bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Gold Nanostars Plus Amikacin against Carbapenem-Resistant Klebsiella pneumoniae Biofilms
Authors: Aguilera-Correa John Jairo , García-Álvarez Rafaela , Mediero Aranzazu , Esteban Jaime , Vallet-Regí María
Published: January 2022

(1) Background: Carbapenem-resistant Klesiella pneumoniae (CR-KP) infection rates depict an almost pre-antibiotic scenario since the pipeline for effective antibiotics against this pathogen has been almost entirely depleted. This study aims to evaluate the antibacterial effect of gold nanostars (GNS) alone or associated with some of the most widely used antibiotics for the treatment of CR-KP strains, i.e., meropenem or amikacin, on both planktonic and sessile forms. Additionally, we measured the effect of GNS on cell proliferation and biocompatibility in invertebrate in vivo models. (2) Materials and methods: GNS were made from gold seeds grown using a seeded-growth surfactant-free method assisted by silver ions and functionalized with mercapto-poly(ethylene glycol)amino by ligand exchange. The antimicrobial capacity, effect on cell proliferation, and biocompatibility of the most effective combination was evaluated in a Galleria mellonella model. (3) Results: The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were 80 and 160 µM of GNS for all strains, respectively. The minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were >320 µM of GNS for both. A synergy was found between GNS and amikacin. Larvae administered GNS plus amikacin were found to tolerate the treatment well, which prevented infection. (4) Conclusions: GNS are a promising anti-CR-KP nanomaterial.
Ozonation of Whole Blood Results in an Increased Release of Microparticles from Blood Cells
Authors: Boczkowska-Radziwon Barbara , Olbromski Piotr Józef , Rogowska Anna , Bujno Magdalena , Myśliwiec Marta , Żebrowska Agnieszka , Średziński Dariusz , Polityńska Barbara , Wojtukiewicz Marek Z. , Radziwon Piotr
Published: January 2022

Autohemotherapy with ozonated blood is used in the treatment of a broad spectrum of clinical disorders. Ozone demonstrates strong oxidizing properties and causes damage to cell membranes. The impact of whole-blood ozonation on the release of microparticles from blood and endothelial cells and the concentration of selected markers in the hemostatic system (APTT, PT, D-dimer, fibrinogen) were investigated. Venous blood, obtained from 19 healthy men, was split into four equal parts and treated with air, 15 µg/mL ozone, or 30 µg/mL ozone, or left untreated. The number and types of microparticles released were determined using flow cytometry on the basis of surface antigen expression: erythrocyte-derived microparticles (CD235+), platelet-derived microparticles (CD42+), leukocyte-derived microparticles (CD45+), and endothelial-derived microparticles (CD144+). The study is the first to demonstrate that ozone induces a statistically significant increase in the number of microparticles derived from blood and endothelial cells. Although statistically significant, the changes in some coagulation factors were somewhat mild and did not exceed normal values.
Synthesis and Structure–Activity Relationships of Novel Non-Steroidal CYP17A1 Inhibitors as Potential Prostate Cancer Agents
Authors: Wróbel Tomasz , Rogova Oksana , Sharma Katyayani , Rojas Velazquez Maria , Pandey Amit , Jørgensen Flemming , Arendrup Frederic , Andersen Kasper , Björkling Fredrik
Published: January 2022

Twenty new compounds, targeting CYP17A1, were synthesized, based on our previous work on a benzimidazole scaffold, and their biological activity evaluated. Inhibition of CYP17A1 is an important modality in the treatment of prostate cancer, which remains the most abundant cancer type in men. The biological assessment included CYP17A1 hydroxylase and lyase inhibition, CYP3A4 and P450 oxidoreductase (POR) inhibition, as well as antiproliferative activity in PC3 prostate cancer cells. The most potent compounds were selected for further analyses including in silico modeling. This combined effort resulted in a compound (comp 2, IC50 1.2 µM, in CYP17A1) with a potency comparable to abiraterone and selectivity towards the other targets tested. In addition, the data provided an understanding of the structure–activity relationship of this novel non-steroidal compound class.
The Validity and Reliability of a Global Navigation Satellite System in Canoe Slalom
Authors: Macdermid Paul W. , Coppelmans Anna , Cochrane Darryl
Published: January 2022

This study investigates the usefulness of a 10 Hz GPS device for tracking scalar performance in canoe slalom through assessing the validity of automated-informed-aerial video tracking (30 fps and 10 fps) and GPS capability in relation to a known track. Additionally, a real-world (canoe-slalom). A comparison between manual-aerial video tracking (10 fps) and the 10 Hz GPS was performed. All three methods of tracking used during the dry-land test (30 fps or 10 fps video and GPS) reported significantly lower distances (−3.2, −5.1 and −8.5%, p < 0.0001) but were deemed useful based on sample rate and body positioning difference. Intra-method reliability was good (CV = 2.5−2.6%) but requires visual inspection for dataset errors. Informed-colour filtered automated tracking on-water was not possible, but manual tracking provided fewer dataset errors than dry-land automated tracking. GPS significantly (p < 0.0001) under reports distance travelled at key moments during real-world slalom with a bias ± SD of 2.26 ± 2.07 m compared to 10 fps manual-aerial video tracking. The aerial video combined with manual tracking proved most suitable for tracking canoe slalom athlete trajectory in a real-world setting but needs to be automated into an application-based package to make it useable for coaches. GPS, as presented, provides insight but does not accurately quantify movements critical in determining the performance of canoe slalom.
Transcriptomic and Lipidomic Mapping of Macrophages in the Hub of Chronic Beta-Adrenergic-Stimulation Unravels Hypertrophy-, Proliferation-, and Lipid Metabolism-Related Genes as Novel Potential Markers of Early Hypertrophy or Heart Failure
Authors: Nadaud Sophie , Flamant Mathilde , Le Goff Wilfried , Balse Elise , Pavoine Catherine
Published: January 2022

Sympathetic nervous system overdrive with chronic release of catecholamines is the most important neurohormonal mechanism activated to maintain cardiac output in response to heart stress. Beta-adrenergic signaling behaves first as a compensatory pathway improving cardiac contractility and maladaptive remodeling but becomes dysfunctional leading to pathological hypertrophy and heart failure (HF). Cardiac remodeling is a complex inflammatory syndrome where macrophages play a determinant role. This study aimed at characterizing the temporal transcriptomic evolution of cardiac macrophages in mice subjected to beta-adrenergic-stimulation using RNA sequencing. Owing to a comprehensive bibliographic analysis and complementary lipidomic experiments, this study deciphers typical gene profiles in early compensated hypertrophy (ECH) versus late dilated remodeling related to HF. We uncover cardiac hypertrophy- and proliferation-related transcription programs typical of ECH or HF macrophages and identify lipid metabolism-associated and Na+ or K+ channel-related genes as markers of ECH and HF macrophages, respectively. In addition, our results substantiate the key time-dependent role of inflammatory, metabolic, and functional gene regulation in macrophages during beta-adrenergic dependent remodeling. This study provides important and novel knowledge to better understand the prevalent key role of resident macrophages in response to chronically activated beta-adrenergic signaling, an effective diagnostic and therapeutic target in failing hearts.
Phase-Based Grasp Classification for Prosthetic Hand Control Using sEMG
Authors: Wang Shuo , Zheng Jingjing , Zheng Bin , Jiang Xianta
Published: January 2022

Pattern recognition using surface Electromyography (sEMG) applied on prosthesis control has attracted much attention in these years. In most of the existing methods, the sEMG signal during the firmly grasped period is used for grasp classification because good performance can be achieved due to its relatively stable signal. However, using the only the firmly grasped period may cause a delay to control the prosthetic hand gestures. Regarding this issue, we explored how grasp classification accuracy changes during the reaching and grasping process, and identified the period that can leverage the grasp classification accuracy and the earlier grasp detection. We found that the grasp classification accuracy increased along the hand gradually grasping the object till firmly grasped, and there is a sweet period before firmly grasped period, which could be suitable for early grasp classification with reduced delay. On top of this, we also explored corresponding training strategies for better grasp classification in real-time applications.
Inflammatory Stress Induced by Intraperitoneal Injection of LPS Increases Phoenixin Expression and Activity in Distinct Rat Brain Nuclei
Authors: Friedrich Tiemo , Schalla Martha Anna , Goebel-Stengel Miriam , Kobelt Peter , Rose Matthias , Stengel Andreas
Published: January 2022

Due to phoenixin’s role in restraint stress and glucocorticoid stress, as well as its recently shown effects on the inflammasome, we aimed to investigate the effects of lipopolysaccharide (LPS)-induced inflammatory stress on the activity of brain nuclei-expressing phoenixin. Male Sprague Dawley rats (n = 6/group) were intraperitoneally injected with either LPS or control (saline). Brains were processed for c-Fos and phoenixin immunohistochemistry and the resulting slides were evaluated using ImageJ software. c-Fos was counted and phoenixin was evaluated using densitometry. LPS stress significantly increased c-Fos expression in the central amygdaloid nucleus (CeM, 7.2-fold), supraoptic nucleus (SON, 34.8 ± 17.3 vs. 0.0 ± 0.0), arcuate nucleus (Arc, 4.9-fold), raphe pallidus (RPa, 5.1-fold), bed nucleus of the stria terminalis (BSt, 5.9-fold), dorsal motor nucleus of the vagus nerve (DMN, 89-fold), and medial part of the nucleus of the solitary tract (mNTS, 121-fold) compared to the control-injected group (p < 0.05). Phoenixin expression also significantly increased in the CeM (1.2-fold), SON (1.5-fold), RPa (1.3-fold), DMN (1.3-fold), and mNTS (1.9-fold, p < 0.05), leading to a positive correlation between c-Fos and phoenixin in the RPa, BSt, and mNTS (p < 0.05). In conclusion, LPS stress induces a significant increase in activity in phoenixin immunoreactive brain nuclei that is distinctively different from restraint stress.
Editorial: “Neuromodulation of Language, Cognition and Emotion”
Authors: Borgomaneri Sara , de Vega Manuel
Published: January 2022

Neuromodulation can be defined as the alteration of brain activity by delivering physical stimuli to a specific neural region [...]
Partitioning Climate, Users, and Thermophysical Uncertainties from Building Energy Use: A Monte Carlo & ANOVA Approach
Authors: Yassaghi Hamed , Mostafavi Nariman , Wen Jin , Hoque Simi
Published: January 2022

Buildings are subject to many uncertainties ranging from thermophysical performance to user activity. Climate change is an additional source of uncertainty that complicates building performance evaluation. This study aims to quantify the share of uncertainties stemming from building factors, user behavior, and climate uncertainty from boilers, chillers, fans, pumps, total HVAC systems, and total site energy use. A novel method combining Monte Carlo analysis and ANOVA is proposed to partition uncertainties from building energy simulation results under different climate change scenarios. The Monte Carlo method is used to generate distributions of building and user factors as building simulation inputs. Then, simulation results under current and future climate conditions are post-processed using a three-way ANOVA technique to discretize the uncertainties for a reference office building in Philadelphia, PA. The proposed method shows the share in percentages of each input factor (building, user, and climate) in the total uncertainty of building energy simulation output results. Our results indicate that the contribution of climate uncertainty increases from current conditions to future climate scenarios for chillers, boilers, fans, and pumps’ electricity use. User parameters are the dominant uncertainty factor for total site energy use and fans’ electricity use. Moreover, boiler and HVAC energy use are highly sensitive to the shape and range of user and building input factor distributions. We underline the importance of selecting the appropriate distribution for input factors when partitioning the uncertainties of building performance modeling.
Continuous-Flow Sunlight-Powered CO2 Methanation Catalyzed by γ-Al2O3-Supported Plasmonic Ru Nanorods
Authors: Rohlfs Jelle , Bossers Koen W. , Meulendijks Nicole , Valega Mackenzie Fidel , Xu Man , Verheijen Marcel A. , Buskens Pascal , Sastre Francesc
Published: January 2022

Plasmonic CO2 methanation using γ-Al2O3-supported Ru nanorods was carried out under continuous-flow conditions without conventional heating, using mildly concentrated sunlight as the sole and sustainable energy source (AM 1.5, irradiance 5.5–14.4 kW·m−2 = 5.5–14.4 suns). Under 12.5 suns, a CO2 conversion exceeding 97% was achieved with complete selectivity towards CH4 and a stable production rate (261.9 mmol·gRu−1·h−1) for at least 12 h. The CH4 production rate showed an exponential increase with increasing light intensity, suggesting that the process was mainly promoted by photothermal heating. This was confirmed by the apparent activation energy of 64.3 kJ·mol−1, which is very similar to the activation energy obtained for reference experiments in dark (67.3 kJ·mol−1). The flow rate influence was studied under 14.4 suns, achieving a CH4 production plateau of 264 µmol min−1 (792 mmol·gRu−1·h−1) with a constant catalyst bed temperature of approximately 204 °C.
Six-Minute Walk Distance Is a Useful Outcome Measure to Detect Motor Decline in Treated Late-Onset Pompe Disease Patients
Authors: Claeys Kristl G. , D’Hondt Ann , Fache Lucas , Peers Koen , Depuydt Christophe E.
Published: January 2022

Late-onset Pompe disease (LOPD) is a rare, progressive disorder characterized by limb–girdle muscle weakness and/or respiratory insufficiency, caused by acid alpha-glucosidase (GAA) gene mutations and treated with enzyme replacement therapy. We studied isometric muscle strength in eight muscle groups bilaterally using a Biodex® dynamometer, as well as the Medical Research Council sum score (MRC-SS), hand grip strength, 6 min walk distance (6MWD), 10 m walk test (10MWT) and timed up-and-go test (TUG) in 12 adult, ambulatory, treated LOPD patients and 12 age-/gender-matched healthy controls, every 6 months for 2 years. The mean isometric muscle strength showed a significant decline in right and left knee extensors at 12 months in controls (p < 0.014; p < 0.016), at 18 months in patients (p < 0.010; p < 0.007) and controls (only right side, p < 0.030) and at 24 months in both groups (p < 0.035). The mean 6MWD in patients significantly decreased after 24 months, from 451.9 m to 368.1 m (p < 0.003), whereas in controls, the mean 6MWD significantly increased after 6 months (p < 0.045) and 18 months (p < 0.020) (at 24 months p = 0.054). In patients and controls, the MRC-SS, hand grip test, 10MWT and TUG did not show significant changes (p > 0.05). We conclude that the 6MWD is a useful outcome measure to detect motor decline in treated LOPD patients.
The Ancient and Evolved Mouse Sperm-Associated Antigen 6 Genes Have Different Biologic Functions In Vivo
Authors: Yap Yi Tian , Li Wei , Zhou Qi , Haj-Diab Sarah , Chowdhury Dipanwita Dutta , Vaishnav Asmita , Harding Pamela , Williams David C. , Edwards Brian F. , Strauss Jerome F. , Zhang Zhibing
Published: January 2022

Sperm-associated antigen 6 (SPAG6) is the mammalian orthologue of Chlamydomonas PF16, an axonemal central pair protein involved in flagellar motility. In mice, two Spag6 genes have been identified. The ancestral gene, on mouse chromosome 2, is named Spag6. A related gene originally called Spag6, localized on mouse chromosome 16, evolved from the ancient Spag6 gene. It has been renamed Spag6-like (Spag6l). Spag6 encodes a 1.6 kb transcript consisting of 11 exons, while Spag6l encodes a 2.4 kb transcript which contains an additional non-coding exon in the 3′-end as well as the 11 exons found in Spag6. The two Spag6 genes share high similarities in their nucleotide and amino acid sequences. Unlike Spag6l mRNA, which is widely expressed, Spag6 mRNA expression is limited to a smaller number of tissues, including the testis and brain. In transfected mammalian cells, SPAG6/GFP is localized on microtubules, a similar localization as SPAG6L. A global Spag6l knockout mouse model was generated previously. In addition to a role in modulating the ciliary beat, SPAG6L has many unexpected functions, including roles in the regulation of ciliogenesis/spermatogenesis, hearing, and the immunological synapse, among others. To investigate the role of the ancient Spag6 gene, we phenotyped global Spag6 knockout mice. All homozygous mutant mice were grossly normal, and fertility was not affected in both males and females. The homozygous males had normal sperm parameters, including sperm number, motility, and morphology. Examination of testis histology revealed normal spermatogenesis. Testicular protein expression levels of selected SPAG6L binding partners, including SPAG16L, were not changed in the Spag6 knockout mice, even though the SPAG16L level was significantly reduced in the Spag6l knockout mice. Structural analysis of the two SPAG6 proteins shows that both adopt very similar folds, with differences in a few amino acids, many of which are solvent-exposed. These differences endow the two proteins with different functional characteristics, even though both have eight armadillo repeats that mediate protein–protein interaction. Our studies suggest that SPAG6 and SPAG6L have different functions in vivo, with the evolved SPAG6L protein being more important. Since the two proteins have some overlapping binding partners, SPAG6 could have functions that are yet to be identified.
Evaluation of the Usefulness of Human Adipose-Derived Stem Cell Spheroids Formed Using SphereRing® and the Lethal Damage Sensitivity to Synovial Fluid In Vitro
Authors: Fuku Atsushi , Taki Yasuhiko , Nakamura Yuka , Kitajima Hironori , Takaki Takashi , Koya Terutsugu , Tanida Ikuhiro , Nozaki Kaori , Sunami Hiroshi , Hirata Hiroaki , Tachi Yoshiyuki , Masauji Togen , Yamamoto Naoki , Ishigaki Yasuhito , Shimodaira Shigetaka , Shimizu Yusuke , Ichiseki Toru , Kaneuji Ayumi , Osawa Satoshi , Kawahara Norio
Published: January 2022

Osteoarthritis (OA) is an irreversible degenerative condition causing bone deformation in the joints and articular cartilage degeneration with chronic pain and impaired movement. Adipose-derived stem cell (ADSC) or crushed adipose tissue injection into the joint cavity reportedly improve knee function and symptoms, including pain. Stem cell spheroids may be promising treatment options due to their anti-inflammatory and enhanced tissue regeneration/repair effects. Herein, to form human ADSC spheroids, we used first SphereRing® (Fukoku Co., Ltd., Ageo, Japan), a newly developed rotating donut-shaped tube and determined their characteristics by DNA microarray of mRNA analysis. The variable gene expression cluster was then identified and validated by RT-PCR. Gene expression fluctuations were observed, such as COL15A1 and ANGPTL2, related to vascular endothelial cells and angiogenesis, and TNC, involved in tissue formation. In addition, multiplex cytokine analysis in the medium revealed significant cytokines and growth factors production increase of IL-6, IL-10, etc. However, ADSC administration into the joint cavity involves their contact with the synovial fluid (SF). Therefore, we examined how SF collected from OA patient joint cavities affect 2D-culture ADSCs and ADSC spheroids and observed SF induced cell death. ADSC spheroids could become promising OA treatment options, although studying the administration methods and consider their interaction with SF is essential.
Essential Roles of Efferent Duct Multicilia in Male Fertility
Authors: Hoque Mohammed , Kim Eunice N. , Chen Danny , Li Feng-Qian , Takemaru Ken-Ichi
Published: January 2022

Cilia are microtubule-based hair-like organelles on the cell surface. Cilia have been implicated in various biological processes ranging from mechanosensation to fluid movement. Ciliary dysfunction leads to a plethora of human diseases, known as ciliopathies. Although non-motile primary cilia are ubiquitous, motile multicilia are found in restricted locations of the body, such as the respiratory tract, the oviduct, the efferent duct, and the brain ventricles. Multicilia beat in a whip-like motion to generate fluid flow over the apical surface of an epithelium. The concerted ciliary motion provides the driving force critical for clearing airway mucus and debris, transporting ova from the ovary to the uterus, maintaining sperm in suspension, and circulating cerebrospinal fluid in the brain. In the male reproductive tract, multiciliated cells (MCCs) were first described in the mid-1800s, but their importance in male fertility remained elusive until recently. MCCs exist in the efferent ducts, which are small, highly convoluted tubules that connect the testis to the epididymis and play an essential role in male fertility. In this review, we will introduce multiciliogenesis, discuss mouse models of male infertility with defective multicilia, and summarize our current knowledge on the biological function of multicilia in the male reproductive tract.
Isolation of Murine Myeloid Progenitor Populations by CD34/CD150 Surface Markers
Authors: Olender Leonid , Thapa Roshina , Gazit Roi
Published: January 2022

Myeloid progenitors are intermediates between Hematopoietic Stem Cells (HSCs) and Myeloid effector progeny. In mouse bone marrow, they are part of the Lineage− cKit+ Sca1− (LK) compartment. To date, most researchers used CD34 and FcγR surface markers for the dissection of this compartment into various populations. Surprisingly, however, this approach does not provide distinct separation by fluorescence-activated cell sorting (FACS). In this study, we suggest using CD150 instead of FcγR. We re-analyzed published single-cell RNA-Seq data and found that CD34/CD150 provides better sub-populations separation, compared to the “classical” CD34/FcγR-based approach. We confirm our findings by independent FACS analysis. We demonstrate comparable differentiation potential of the newly-obtained LK sub-populations, like previous “classical” ones. Therefore, we suggest the CD34/CD150 gating strategy, utilizing commonly-used surface markers, as a robust and reproducible separation of the LK compartment into distinct sub-populations.
Notch Signaling in HSC Emergence: When, Why and How
Authors: Thambyrajah Roshana , Bigas Anna
Published: January 2022

The hematopoietic stem cell (HSC) sustains blood homeostasis throughout life in vertebrates. During embryonic development, HSCs emerge from the aorta-gonads and mesonephros (AGM) region along with hematopoietic progenitors within hematopoietic clusters which are found in the dorsal aorta, the main arterial vessel. Notch signaling, which is essential for arterial specification of the aorta, is also crucial in hematopoietic development and HSC activity. In this review, we will present and discuss the evidence that we have for Notch activity in hematopoietic cell fate specification and the crosstalk with the endothelial and arterial lineage. The core hematopoietic program is conserved across vertebrates and here we review studies conducted using different models of vertebrate hematopoiesis, including zebrafish, mouse and in vitro differentiated Embryonic stem cells. To fulfill the goal of engineering HSCs in vitro, we need to understand the molecular processes that modulate Notch signaling during HSC emergence in a temporal and spatial context. Here, we review relevant contributions from different model systems that are required to specify precursors of HSC and HSC activity through Notch interactions at different stages of development.
Patterning Large-Scale Nanostructured Microarrays on Coverslip for Sensitive Plasmonic Detection of Aqueous Gliadin Traces
Authors: Casari Bariani Giuliocesare , Zhou Lan , Poggesi Simone , Manzano Marisa , Ionescu Rodica Elena
Published: January 2022

User-friendly devices for detecting low gliadin content in commercial foods are of extreme importance for people with gluten diseases. With this concern, the present work proposes a rapid and sensitive optical nanostructured microarrays platform for the detection of gliadin using specific anti-gliadin IgG antibodies immobilized on annealed gold nanostructures (AuNPs) obtained after the high annealing process (550 °C) of gold thin films evaporated on commercial glass coverslips. Localized Surface Plasmon Resonance (LSPR) immunosensing of gliadin in the range of 0.1 ppm to 1000 ppm is successfully achieved. In addition, the biofunctionalization protocol was used for gluten screening in five food complex products.
The Behavioral Effects of Montessori Pedagogy on Children’s Psychological Development and School Learning
Authors: Gentaz Edouard , Richard Sylvie
Published: January 2022

This review examines the quantitative behavioural studies that have evaluated the effects of Montessori pedagogy on children’s psychological development and school learning. The analyses of only three “Randomized Controlled Trials—RCT” studies published to date reveal varied and contradictory effects. Firstly, these findings are discussed in the light of several methodological limitations: the absence of active control groups, small sample sizes, diversity of measures, or lack of control over the implementation fidelity of both Montessori and conventional pedagogy. Secondly, these findings are discussed in the light of what Montessori pedagogy does not emphasise in its conception of development and the role of the teacher, namely the place given to language and pretend play.
Association of Consuming Tap Water or Purified Water during Infancy with Irritable Bowel Syndrome in Children
Authors: Kim Ju Hee , Baek Hey-Sung , Ha Eun Kyo , Cha Hye Ryung , Lee Seung Won , Han Man Yong
Published: January 2022

Objective: The objective of this study was to analyze the effect of consuming formula powder prepared with tap water or purified water during the first 4 to 6 months of life on the subsequent development of irritable bowel syndrome (IBS). Study design and setting: A total of 917,707 children who were born in Korea between 2007 and 2008 were analyzed. All children were followed up until they lost eligibility for health care services or until 2017. Data on the water used to prepare formula powder were from questionnaires answered by the parents when the child was 4 to 6 months old. IBS was defined as two or more diagnoses of IBS after the age of 4 years. Inverse probability of treatment weighting (IPTW) using the propensity score was used to balance the two groups. The risk of IBS was evaluated using a Cox proportional hazards model. Results: After weighting, there were 73,355 children in the tap water group and 73,351 in the purified water group. The purified water group had a higher risk of IBS (HR: 1.05; 95% CI: 1.01, 1.09). This relationship was also present after the subgroup analyses of males and females and the sensitivity analysis that used different definitions of IBS. Conclusions: Drinking formula powder prepared with purified water rather than tap water during the first 4 to 6 months of age was found to be associated with IBS.
The Effect of Lactobacillus plantarum Extracellular Vesicles from Korean Women in Their 20s on Skin Aging
Authors: Jo Chan Song , Myung Cheol Hwan , Yoon Yeo Cho , Ahn Beom Hee , Min Jin Woo , Seo Won Sang , Lee Dong Hwan , Kang Hee Cheol , Heo Yun Hoe , Choi Hyeong , Hong In Ki , Hwang Jae Sung
Published: January 2022

Extracellular vesicles, which are highly conserved in most cells, contain biologically active substances. The vesicles and substances interact with cells and impact physiological mechanisms. The skin is the most external organ and is in direct contact with the external environment. Photoaging and skin damage are caused by extrinsic factors. The formation of wrinkles is a major indicator of skin aging and is caused by a decrease in collagen and hyaluronic acid. MMP-1 expression is also increased. Due to accruing damage, skin aging reduces the ability of the skin barrier, thereby lowering the skin’s ability to contain water and increasing the amount of water loss. L. plantarum suppresses various harmful bacteria by secreting an antimicrobial substance. L. plantarum is also found in the skin, and research on the interactions between the bacteria and the skin is in progress. Although several studies have investigated L. plantarum, there are only a limited number of studies on extracellular vesicles (EV) derived from L. plantarum, especially in relation to skin aging. Herein, we isolated EVs that were secreted from L. plantarum of women in their 20s (LpEVs). We then investigated the effect of LpEVs on skin aging in CCD986sk. We showed that LpEVs modulated the mRNA expression of ECM related genes in vitro. Furthermore, LpEVs suppressed wrinkle formation and pigmentation in clinical trials. These results demonstrated that LpEVs have a great effect on skin aging by regulating ECM related genes. In addition, our study offers important evidence on the depigmentation effect of LpEVs.
Reconstruction Method of Old Well Logging Curves Based on BI-LSTM Model—Taking Feixianguan Formation in East Sichuan as an Example
Authors: Cheng Chao , Gao Yan , Chen Yan , Jiao Shixiang , Jiang Yuqiang , Yi Juanzi , Zhang Liang
Published: January 2022

In order to define a favorable oil and gas accumulation area, this study focused on reservoir recognition which is based on logging data of old wells. The Gaofengchang structure in eastern Sichuan is used as a test area to discuss the necessity and feasibility of curve construction by combining new and old wells. Analysis of the reasons for the inaccuracy of the traditional curve reconstruction method is also provided. Given the interdependence of the well log in the depth domain sample sequence, a new intelligent construction method (BI-LSTM) based on the cyclic neural network is proposed. A discussion on the effect of data increments on prediction accuracy is also provided. The following four conclusions were achieved through curve reconstruction experiments: a high-precision CNL pseudo-curve was obtained; an underdetermined equation in optimization logging interpretation method needed to be extended to a positive definite equation; the quantitative processing of the complex lithologic reservoir parameters for the old wells was realized; and the processing result of the lithology physical property were basically consistent with the core data. Therefore, the BI-LSTM proposed in this paper could improve the accuracy of logging curve construction and has a good promotion significance for the old well review.
Preparation and Enhanced Isothermal Oxidation Resistance of a Low Diffusivity NiRePtAl Single-Phase Coating
Authors: Liu He , Yang Erqi , Guo Cean , Liu Quan , Yang Lanlan , Bao Zebin
Published: January 2022

A low diffusivity NiRePtAl single-phase coating was formed on a Ni3Al-based SC superalloy by electroplating and aluminizing treatments, in which the electroplating consisted of depositing Ni-Re and Pt layer. The isothermal oxidation test of the sample was evaluated at 1100 and 1200 °C; the results indicated that the low diffusivity NiRePtAl single-phase sample promoted the oxidation resistance due to a greater β-NiAl phases-enriched outer layer. The Re-base diffusion barrier could effectively refrain the outward diffusion of Mo. Low diffusivity NiRePtAl single-phase coating has a lower inter-diffusion rate to the superalloy, where the thickness of the secondary reaction zone decreased by 35%. Mechanisms responsible for improved oxidation resistance and decreased extent for the formation of secondary reaction zones are discussed in the present study.
Clinical Application and Biological Functionalization of Different Surface Coatings in Artificial Joint Prosthesis: A Comprehensive Research Review
Authors: Yang Xu , Gao Fuqiang , Sun Wei , Li Zirong
Published: January 2022

With advances in materials science and biology, there have been continuing innovations in the field of artificial joint prostheses. Cementless prostheses have the advantages of long service life, easy revision, and good initial stability and are widely used in artificial joint replacement. Coatings are the key to cementless prostheses and are at the heart of their excellent functionality. This article mainly studies the clinical application of hydroxyapatite (HA) coating, standard porous coating represented by Porocoat coating, and new high-porosity coating represented by Gription coating. The clinical application and biological functionalization of different artificial joint prosthesis surface coatings are clarified, and it provides a reference for the clinical selection and development of different prosthesis surface coating materials.
Development and Characterization of Emulsions Containing Ground Seeds of Passiflora Species as Biobased Exfoliating Agents
Authors: Linares-Devia Natalia , Arrieta-Escobar Javier , Baena Yolima , Orjuela Alvaro , Osorio Coralia
Published: January 2022

Ground seeds from three species of the genus Passiflora, P. ligularis, P. edulis Sims fo edulis, and P. mollissima, were obtained by extraction, drying, grinding, and sieving, and their physicochemical properties (morphology, hardness, and proximal analysis) were compared to those of commercial exfoliant seeds from passion fruit. Particle sizes between 0.5 and 1 mm were obtained, and their properties were similar to the commercial product except for the extractable material content that was higher. Subsequently, prototypes of an exfoliating cosmetic product were developed by using the ground seeds as the main active ingredient. Rheology characterization of samples enables to verify that the particles have minor effects on emulsion properties and that the emulsion is stable even after thermal treatment. In particular, the pH of the emulsion decreased when using the obtained ground seeds. This is consistent with the extraction and solvation of organic acids into the emulsion, in particular, alpha-hydroxy acids, which are present in high concentrations in Passiflora species. This indicates that the prepared emulsions could have a synergic chemical and physical exfoliating activity and could be used in cosmetic products.
Multicomponent Solids of DL-2-Hydroxy-2-phenylacetic Acid and Pyridinecarboxamides
Authors: Castiñeiras Alfonso , Frontera Antonio , García-Santos Isabel , González-Pérez Josefa M. , Niclós-Gutiérrez Juan , Torres-Iglesias Rocío
Published: January 2022

We prepared cocrystals of DL-2-Hydroxy-2-phenylacetic acid (D, L-H2ma) with the pyridinecarboxamide isomers, picolinamide (pic) and isonicotinamide (inam). They were characterized by elemental analysis, single crystal and powder X-ray, IR spectroscopy and 1H and 13C NMR. The crystal and molecular structures of (pic)-(D-H2ma) (1), (nam)-(L-H2ma) (2) and (inam)-(L-H2ma) (3) were studied. The crystal packing is stabilized primarily by hydrogen bonding and in some cases through π-π stacking interactions. The analysis of crystal structures reveals the existence of the characteristic heterosynthons with the binding motif R22(8) (primary amide–carboxilic acid) between pyridinecarboxamide molecules and the acid. Other synthons involve hydrogen bonds such as O-H(carboxyl)···N(pyridine) and O-H(hydroxyl)···N(pyridine) depending on the isomer. The packing of 1 and 3 is formed by tetramers, for whose formation a crystallization mechanism based on two stages is proposed, involving an amide–acid (1) or amide–amide (3) molecular recognition in the first stage and the formation of others, and interdimeric hydrogen bonding interactions in the second. The thermal stability of the cocrystals was studied by differential scanning calorimetry and thermogravimetry. Further studies were conducted to evaluate other physicochemical properties of the cocrystals in comparison to the pure coformers. Density-functional theory (DFT) calculations (including NCIplot and QTAIM analyses) were performed to further characterize and rationalize the noncovalent interactions.
Fumarate Based Metal–Organic Framework: An Effective Catalyst for the Transesterification of Used Vegetable Oil
Authors: Shaik Mohammed Rafi , Adil Syed Farooq , ALOthman Zeid A. , Alduhaish Osamah M.
Published: January 2022

Advancement of technology for the sustainable production of biodiesel is of significant importance in fighting against rising fuel costs due to the fast depletion of fossil fuels. In this regard, the application of highly efficient MOFs (metal–organic frameworks)-based materials as acidic, basic, or supported heterogeneous catalysts plays a crucial role in enhancing the efficiency of biodiesel production processes. In this report, we demonstrate the synthesis and catalytic application of Zr-fumarate-MOF (also known as MOF-801) as a heterogeneous catalyst for the transesterification reaction of used vegetable oil (UVO) for the production of biodiesel. The formation of MOF-801 and its structural stability is confirmed by a variety of characterization techniques including XRD, SEM, EDX, FT-IR, BET, and TGA analyses. The results revealed the formations of highly crystalline, cubic MOF-801 possessing thermal stability below 500 °C. The MOF-801 catalyst demonstrated moderate catalytic activity during transesterification of UVO (~60%) at 50 wt.% of methanol: oil, 10 wt.% catalyst loading, 180 °C reaction temperature, and 8 h of reaction time. Furthermore, the catalyst has exhibited adequate reusability with a slight reduction in the reaction yield of up to ~10% after three cycles.
Modeling of Chloride Binding Capacity in Cementitious Matrices Including Supplementary Cementitious Materials
Authors: Abd El Fattah Ahmed M. , Al-Duais Ibrahim N. A.
Published: January 2022

The improvement in the chloride binding capacity of concrete has been shown to increase corrosion resistance. The addition of supplementary cementitious materials (SCMs) to Portland cement has been proven to increase the binding capacity, except for silica fume, whereas the impact of chemical additives is not extensively addressed in the literature. This work studies the influence of SCMs and chemical additives, i.e., calcium nitrite inhibitor (CNI), migrating corrosion inhibitor (MCI), and Caltite as a hydrophobic material, on binding capacity. The addition of both corrosion inhibitors (MCI and CNI) has minimal effect on the binding capacity, while the addition of Caltite reduces the binding capacity by limiting the contact of the samples with the salt in water due to its hydrophobic nature. In addition, the study compares the performance of the available fitting–binding models against the available experimental work in the literature, and shows that the Freundlich isotherm is the best fitting model for describing the relationship between the binding capacity and the free chloride. The study further relates the binding capacity to different compositions in cement and SCMs, and shows, by conducting quantitative analysis, that the Al2O3 content is the dominant factor affecting the binding capacity. Finally, this work proposes a new model, which uses Al2O3 content and free salt concentration to predict the bound chloride. The model shows adequate correlations to the experimental work and, further, can be used in service-life modeling of concrete.
#PraCegoVer: A Large Dataset for Image Captioning in Portuguese
Authors: dos Santos Gabriel Oliveira , Colombini Esther Luna , Avila Sandra
Published: January 2022

Automatically describing images using natural sentences is essential to visually impaired people’s inclusion on the Internet. This problem is known as Image Captioning. There are many datasets in the literature, but most contain only English captions, whereas datasets with captions described in other languages are scarce. We introduce the #PraCegoVer, a multi-modal dataset with Portuguese captions based on posts from Instagram. It is the first large dataset for image captioning in Portuguese. In contrast to popular datasets, #PraCegoVer has only one reference per image, and both mean and variance of reference sentence length are significantly high, which makes our dataset challenging due to its linguistic aspect. We carry a detailed analysis to find the main classes and topics in our data. We compare #PraCegoVer to MS COCO dataset in terms of sentence length and word frequency. We hope that #PraCegoVer dataset encourages more works addressing the automatic generation of descriptions in Portuguese.
Neonatal Sepsis and Hemostasis
Authors: Gialamprinou Dimitra , Mitsiakos Georgios , Katsaras Georgios N. , Kontovazainitis Christos-Georgios , Karagianni Paraskevi , Roilides Emmanuel , Kenet Gili
Published: January 2022

Neonatal sepsis is considered critical for a significant increase in neonatal morbidity and mortality among hospitalized neonates. Neonatal sepsis, in most cases, coexists with coagulopathy, which can prove to be life-threatening. Complex molecular and cellular systems are involved in the cross-talk between inflammation and hemostasis during sepsis. Disturbances in the regulating systems of the vascular endothelium, and platelet–endothelial and platelet–neutrophil interactions play a pivotal role in both inflammation and coagulation. This complex process is poorly understood in neonates. In addition to the developmental maturation of hemostasis and the immune response in neonatal sepsis, a cellular model of hemostasis during sepsis should be taken into account. This review focused on the molecular and cellular mechanisms underlying inflammation and hemostasis during neonatal sepsis, taking the developmental immune response and developmental hemostasis into account in order to provide future diagnostic approaches to be applied in everyday clinical settings. Regarding the diagnostic modalities, we briefly provide the limitations of the currently used conventional coagulation assays, focusing on viscoelastic tests and platelet flow cytometry.
Comparison of Tongue Characteristics Classified According to Ultrasonographic Features Using a K-Means Clustering Algorithm
Authors: Chantaramanee Ariya , Nakagawa Kazuharu , Yoshimi Kanako , Nakane Ayako , Yamaguchi Kohei , Tohara Haruka
Published: January 2022

The precise correlations among tongue function and characteristics remain unknown, and no previous studies have attempted machine learning-based classification of tongue ultrasonography findings. This cross-sectional observational study aimed to investigate relationships among tongue characteristics and function by classifying ultrasound images of the tongue using a K-means clustering algorithm. During 2017–2018, 236 healthy older participants (mean age 70.8 ± 5.4 years) were enrolled. The optimal number of clusters determined by the elbow method was 3. After analysis of tongue thickness and echo intensity plots, tongues were classified into three groups. One-way ANOVA was used to compare tongue function, tongue pressure, and oral diadochokinesis for /ta/ and /ka/ in each group. There were significant differences in all tongue functions among the three groups. The worst function was observed in patients with the lowest values for tongue thickness and echo intensity (tongue pressure [P = 0.023], /ta/ [P = 0.007], and /ka/ [P = 0.038]). Our results indicate that ultrasonographic classification of tongue characteristics using K-means clustering may aid clinicians in selecting the appropriate treatment strategy. Indeed, ultrasonography is advantageous in that it provides real-time imaging that is non-invasive, which can improve patient follow-up both in the clinic and at home.
The Influence of the COVID-19 Pandemic on Orthodontic Treatments: A Survey Analysis
Authors: Saccomanno Sabina , Saran Stefano , Guercio Elisabetta , Mastrapasqua Rodolfo Francesco , Pirino Alessio , Scoppa Fabio
Published: January 2022

Coronavirus disease 2019 represents the pandemic of the 21st century that has negatively affected the lives of the whole of humanity. For many months, the only weapons to fight against this infection were protective masks and social isolation. During recent months, fear of the virus has led people to avoid crowded environments and events, and to reduce medical checks, limiting them only to emergencies. Outpatient clinics, doctors’ offices, and all closed-in environments were required to limit the patients’ access. Nowadays, the presence of specific protocols around the world, and the extended vaccination campaign, have allowed a reduction of many restrictions. Unfortunately, the virus is still widespread, and dental practice and dental treatments suffer the consequences. Dental therapies in general, and in particular orthodontics, are not considered lifesaving. Due to this, orthodontists, in this historical time, must find solutions for motivating patients to start or continue therapies, while providing a safe way for them to do so. There are orthodontists who have developed, during this period, different ways to help them in treating and communicating with patients. Aim: The aim of this study is to assess the influence of the pandemic on the choice to start orthodontic treatment, oral health care, and the importance placed on the appearance of dental occlusions. Materials and Methods: This study is a survey analysis of 159 people, which was posted in Facebook groups of adult orthodontic patients. The timestamps and answers of responses were analyzed to avoid duplicated or interrupted questionnaires. Conclusions: This study found that the current health emergency has not reduced the demand for orthodontic care, while some patients’ behaviors are changing in relation to oral hygiene and the importance that they attribute to dental health. It seems that dentists’ availability plays a key role in this period of sanitary emergency.
Distributed 3D Navigation of Swarms of Non-Holonomic UAVs for Coverage of Unsteady Environmental Boundaries
Authors: Matveev Alexey S. , Semakova Anna A.
Published: January 2022

A team of non-holonomic constant-speed under-actuated unmanned aerial vehicles (UAVs) with lower-limited turning radii travel in 3D. The space hosts an unknown and unpredictably varying scalar environmental field. A space direction is given; this direction and the coordinate along it are conditionally termed as the “vertical” and “altitude”, respectively. All UAVs should arrive at the moving and deforming isosurface where the field assumes a given value. They also should evenly distribute themselves over a pre-specified range of the “altitudes” and repeatedly encircle the entirety of the isosurface while remaining on it, each at its own altitude. Every UAV measures only the field intensity at the current location and both the Euclidean and altitudinal distances to the objects (including the top and bottom of the altitudinal range) within a finite range of visibility and has access to its own speed and the vertical direction. The UAVs carry no communication facilities, are anonymous to one another, and cannot play distinct roles in the team. A distributed control law is presented that solves this mission under minimal and partly inevitable assumptions. This law is justified by a mathematically rigorous global convergence result; computer simulation tests confirm its performance.