bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Aromatic Ring Quaternary Ammonium and Phosphonium Salts–Carboxylic Acids-Based Deep Eutectic Solvent for Enhanced Sugarcane Bagasse Pretreatment, Enzymatic Hydrolysis, and Cellulosic Ethanol Production
Authors: Li Biying , Qiu Ziqi , Huang Jiale , Xian Xiaoling , Zheng Xiaojie , Lin Xiaoqing
Published: November 2023

Deep eutectic solvents (DESs) with a hydrophobic aromatic ring structure offer a promising pretreatment method for the selective delignification of lignocellulosic biomass, thereby enhancing enzymatic hydrolysis. Further investigation is needed to determine whether the increased presence of aromatic rings in hydrogen bond receptors leads to a more pronounced enhancement of lignin removal. In this study, six DES systems were prepared using lactic acid (LA)/acetic acid (AA)/levulinic acid (LEA) as hydrogen bond donors (HBD), along with two independent hydrogen bond acceptors (HBA) (benzyl triethyl ammonium chloride (TEBAC)/benzyl triphenyl phosphonium chloride (BPP)) to evaluate their ability to break down sugarcane bagasse (SCB). The pretreatment of the SCB (raw material) was carried out with the above DESs at 120 °C for 90 min with a solid–liquid ratio of 1:15. The results indicated that an increase in the number of aromatic rings may result in steric hindrance during DES pretreatment, potentially diminishing the efficacy of delignification. Notably, the use of the TEBAC:LA-based DES under mild operating conditions proved highly efficient in lignin removal, achieving 85.33 ± 0.52% for lignin removal and 98.67 ± 2.84% for cellulose recovery, respectively. The maximum digestibilities of glucan (56.85 ± 0.73%) and xylan (66.41 ± 3.06%) were attained after TEBAC:LA pretreatment. Furthermore, the maximum ethanol concentration and productivity attained from TEBAC:LA-based DES-pretreated SCB were 24.50 g/L and 0.68 g/(L·h), respectively. Finally, the comprehensive structural analyses of SCB, employing X-rays, FT-IR, and SEM techniques, provided valuable insights into the deconstruction process facilitated by different combinations of HBDs and HBAs within the DES pretreatment.
Influence of Fermentation Time on the Chemical and Functional Composition of Different Cocoa Clones from Southern Colombia
Authors: Polanía-Hincapié Paola Andrea , Suárez Juan Carlos , Hernández Héctor Eduardo , Ramón-Triana Vivian Yorlady , Cuéllar-Álvarez Liceth N. , Casanoves Fernando
Published: November 2023

Cocoa bean quality depends mainly on genetic material, edaphoclimatic factors and post-harvest processes such as fermentation. The impact of the fermentation process on the chemical and functional composition of different cocoa clones grown in southern Colombia was analyzed. A factorial design with repeated measures over time was used to analyze the effect of clone and fermentation time on chemical characteristics (bromatology, phenolic compounds and antioxidant activity). The bromatological characteristics showed significant differences between clones and fermentation time. Clones EET-8 and CCN-51 showed higher contents of acidity (0.51%), fat (63.61%), protein (12.85%) and carbohydrates (1.63 mg g−1). Moisture, acidity and sucrose increased their values between day 4 and day 6 of fermentation (p < 0.05). Phenolic compounds and antioxidant activity had significant differences between clones, where clones CCN-51 and ICS-95 had high contents of phenols (64.56 mg g−1), flavonoids (3.30 mg g−1) and DPPH reducing capacity (325.55 µmol g−1). In this sense, we consider the FSV-41 clone as the major grain quality index based on the results of chemical composition at the bromatological level, antioxidant activity and phenolic compounds.
The Destructive Sir Ivan Fire in New South Wales, Australia; Simulations Using a Coupled Fire—Atmosphere Model
Authors: Peace Mika , Ye Hua , Greenslade Jesse , Kepert Jeffrey D.
Published: November 2023

The destructive Sir Ivan Dougherty fire burned 55,000 hectares around 250 km northwest of Sydney in New South Wales on 12 February 2017. Record hot temperatures were recorded in the area during the lead-in days and the fire conditions at the time were described as the ‘worst ever seen in NSW’. The observed weather conditions were hot, dry and very windy ahead of a synoptic frontal wind change during the afternoon. ‘Extreme’ to ‘catastrophic’ fire weather was predicted, and the potential for extreme fire behaviour was identified several days in advance. The Australian coupled fire–atmosphere model ACCESS-Fire has been run to explore the characteristics of the Sir Ivan fire. Several features resulting from fire–atmosphere interaction are produced in the simulations. Simulated heat flux along the fire perimeter shows increased intensity on the northern fire flank in response to gradual backing winds ahead of the main frontal wind change. Temporal and spatial variability in fire activity, seen as pulses in fire intensity and fireline wind speed, develop in response to boundary layer rolls in the wind fields. Deep moist convection consistent with the observed pyrocumulonimbus (pyroCb) cloud is simulated over the fire at around the time of the frontal wind change, and matches guidance from the ‘PyroCb Firepower Threshold’ tool, which showed transient favourable conditions. After the wind change, short-lived near-surface and elevated vortices suggest organised rotating features on the northern flank of the fire. The coupled model captures processes that cannot be produced in uncoupled fire predictions and that are not captured in current operational meteorological forecast products provided to Australian fire agencies. This paper links the features from coupled simulations to available observations and suggests pathways to embed the learnings in operational practice.
Fire and Rescue Services Reconfiguration for Better Dealing with Post-Flashover Building Fires
Authors: Ivanov Martin Lyubomirov , Chow Cheuk Lun
Published: November 2023

The reconfiguration of fire and rescue services is the focus of this research. The main purpose is to propose an alternative organizational model for better dealing with post-flashover building fires. The primary source of empirical information is a survey involving 267 managers from the Bulgarian fire and rescue services to gather insights. The research methodology meticulously encompasses the identification of changes concerning the efficiency and effectiveness of the fire and rescue services, the discernment of inherent challenges, the definition of specific factors and criteria relevant to their activities, and the determination of strategic priorities. The goal is to suggest a new, more efficient way to organize these services in Bulgaria, whose approach can be applicable to other contexts in similar conditions as well. The proposed model suggests that the Bulgarian fire and rescue services should work independently from the Ministry of Interior as the State Agency under the Council of Ministers. In addition, a comparison between this newly proposed model and the current one is conducted to highlight its potential advantages.
Influence of Weber Number on Crown Morphology during an Oblique Droplet Impact on a Thin Wall Film
Authors: Stober Jonathan Lukas , Santini Maurizio , Schulte Kathrin
Published: November 2023

Spray impacts can be found in several technical applications and consist of many single droplets, which impact under different trajectories on wetted walls. This study investigates the asymmetric crown morphology resulting from an oblique impact (α= 60°) of a single droplet on a horizontal and quiescent wall film of the same liquid. A droplet generator with an accelerated needle releases the droplets (D= 1.5 mm) in a controlled trajectory on a thin film (hf/D= 0.2). The impact process is recorded from two perspectives with two synchronized high-speed cameras. Varying the Weber number within the splashing regime reveals distinct crown morphologies, which are described in detail. For We< 500, a single central finger develops at the front of the crown, with subsequent detachments of secondary droplets. At higher We (>500), a collision of the crown with the wall film shortly after impact introduces disturbances into the rim, leading to two fingers in the middle of the front crown. A further increase in We (>600) intensifies the crown–film interaction, resulting in an early ejection of tiny droplets and a complete breakup of the front rim. The influence of We on the crown morphology during an oblique impact is also compared to the normal impact (90°). This study paves the way for a classification of impact regimes and a comprehensive picture of the oblique impact process, which deserve more investigation.
Organic Medicinal and Aromatic Plants: Consumption Profile of a Portuguese Consumer Sample
Authors: Mendes Ana , Oliveira André , Lameiras Jorge , Mendes-Moreira Pedro , Botelho Goreti
Published: November 2023

The production and consumption of organic products have been increasing in Portugal, as well as in the European Union as a whole. The main objective of this work is to understand the consumption habits of organic medicinal and aromatic plants (OMAPs) among Portuguese adults. An online questionnaire was distributed using social networks, resulting in the collection and statistical analysis of 300 responses. Of the participants who reported consuming OMAPs, 44.3% showed a daily consumption pattern. The most frequently mentioned OMAPs for fresh consumption were parsley (Petrosselinum crispum L., 92%), garlic (Allium sativum L., 84.1%), and coriander (Coriandrum sativum L., 78.1%). The most commonly mentioned OMAP for consumption as dried plants were oregano (Origanum vulgare L., 74.6%), lemon balm (Melissa officinalis L., 49.2%), and lemon verbena (Aloysia citrodora L., 46.8%). The main reasons cited for using OMAPs were their benefits to health (58.7% of participants), benefits to the environment (33.2%), and reduced salt consumption (29.5%). Among these, the main health benefits mentioned included anti-inflammatory properties (45.0%), prevention of cardiovascular diseases (41.6%), and prevention of high cholesterol (39.9%). Furthermore, 82.5% of respondents considered themselves sufficiently, well, or excellently informed about the nutritional properties of OMAPs. This research initiates a discussion about whether profiling OMAP consumption habits can serve as a valuable tool for promoting organic farming in Portugal, increasing OMAP production and consumption, and strengthening the connection between these products and potential positive human health effects.
Enhancing the Physicochemical Attributes of Dough and Noodles through the Incorporation of Bacillus vallismortis Laccase
Authors: Zhu Xiaoyu , Zhang Shijin , Bian Luyao , Shen Juan , Zhang Chong , Manickam Sivakumar , Tao Yang , Lu Zhaoxin
Published: November 2023

This investigation examined how the Bacillus vallismortis laccase (rBVL-MRL522) influenced the physicochemical characteristics, structural attributes, and functional capabilities of both dough and noodles. Incorporating rBVL-MRL522 (1 U/g) did not lead to a substantial change in the water absorption of wheat flour. However, the introduction of rBVL-MRL522 caused a significant elongation in the formation time of wheat flour dough, extending it by 88.9%, and also resulted in a 50% increase in the stabilization duration of wheat flour dough. Furthermore, adding rBVL-MRL522 led to a proportional rise in both the elastic and viscous moduli (G’’ of the dough, signifying that r-BVL (rBVL-MRL522) has a beneficial effect on the gluten strength of the dough. Integrating rBVL-MRL522 promoted the consolidation of the gluten-based cross-linked structure within the dough, decreasing the size of starch particles and, more evenly, the dispersion of these starch particles. In the noodle processing, adding rBVL-MRL522 at a rate of 1 U/g raised the L* value of the noodles by 2.34 units compared to the noodles prepared without the inclusion of rBVL-MRL522. Using a greater amount of rBVL-MRL522 (2 U/g) substantially increased the hardness of the noodles by 51.31%. Additionally, rBVL-MRL522 showed a noteworthy enhancement in the elasticity, cohesiveness, and chewiness of the noodles. In conclusion, rBVL-MRL522 promoted the cross-linking gluten, leading to a more extensive and condensed three-dimensional network structure in raw and cooked noodles. As a result, this study offers valuable insights into the environmentally friendly processing of dough and associated products.
The Progressive Utilization of Ponkan Peel Residue for Regulating Human Gut Microbiota through Sequential Extraction and Modification of Its Dietary Fibers
Authors: Gao Pu , Zheng Meiyu , Lu Hanyu , Lu Shengmin
Published: November 2023

As a by-product of citrus processing, ponkan (Citrus reticulata Blanco, cv. Ponkan) peel residue is a source of high quality dietary fiber (DF). To make a full utilization of this resource and give a better understanding on the probiotic function of its DF, soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were extracted from ponkan peel residue (after flavonoids were extracted) using an alkaline method, followed by modifications using a composite physical-enzymatic treatment. The in vitro fermentation properties of the modified SDF and IDF (namely, MSDF and MIDF) and their effects on short-chain fatty acids (SCFA) production and changes in the composition of human gut microbiota were investigated. Results showed that MSDF and MIDF both significantly lowered the pH value and enhanced total SCFA content in the broths after fermented for 24 h by fecal inocula (p < 0.05) with better effects found in MSDF. Both MSDF and MIDF significantly reduced the diversity, with more in the latter than the former, and influenced the composition of human gut microbiota, especially increasing the relative abundance of Bacteroidetes and decreasing the ratio of Firmicutes to Bacteroidetes (F/B) value. The more influential microbiota by MSDF were g-Collinsella, p-Actinobacteria and g-Dialister, while those by MIDF were f-Veillonellaceae, c-Negativicutes and f-Prevotellacese. These results suggested that the modified ponkan peel residue DF can be utilized by specific bacteria in the human gut as a good source of fermentable fiber, providing a basis for the exploitation of the citrus by-product.
Design of Recyclable Carboxylic Metal-Organic Framework/Chitosan Aerogels for Oil Bleaching
Authors: Jia Xiang-Ze , Yao Qing-Bo , Zhang Bin , Tan Chin-Ping , Zeng Xin-An , Huang Yan-Yan , Huang Qiang
Published: November 2023

Novel hierarchical metal-organic framework/chitosan aerogel composites were developed for oil bleaching. UiO-66-COOH-type metal organic frameworks (Zr-MOFs) were synthesized and integrated onto a chitosan matrix with different contents and named MOF-aerogel-1 and MOF-aerogel-2. Due to the compatibility of chitosan, the carboxylic zirconium MOF-aerogels not only maintained the inherent chemical accessibility of UiO-66-COOH, but the unique crystallization and structural characteristics of these MOF nanoparticles were also preserved. Through 3-dimensional reconstructed images, aggregation of the UiO-66-COOH particles was observed in MOF-aerogel-1, while the MOF was homogeneously distributed on the surface of the chitosan lamellae in MOF-aerogel-2. All aerogels, with or without immobilized MOF nanoparticles, were capable of removing carotenoids during oil bleaching. MOF-aerogel-2 showed the most satisfying removal proportions of 26.6%, 36.5%, and 47.2% at 50 °C, 75 °C, and 100 °C, respectively, and its performance was very similar to that of commercial activated clay. The reuse performance of MOF-aerogel-2 was tested, and the results showed its exceptional sustainability for carotenoid removal. These findings suggested the effectiveness of the MOFaerogel for potential utilization in oil bleaching treatments.
Establishing the Manner of Death: A 3D Reconstruction of a Case of Hanging
Authors: Benevento Marcello , Ambrosi Laura , Maselli Eloisa , Ferorelli Davide , Solarino Biagio , Di Fazio Aldo
Published: November 2023

Establishing the manner of death is one of the most challenging tasks for forensic pathologists. We present the case of a 24-year-old woman found dead in the early morning on a flyover. The body was sitting on the ground with the back leaning against a wall. The neck was encircled by a white phone charger cable knotted to the staircase’s handrail. The victim had argued with her boyfriend and tried to jump out of his car while coming home from a wedding party the night before. After that, she left home alone with her phone charger in her hand. Due to self-harm behaviors, the first hypothesis was suicide by hanging. However, the ligature crossed immediately beneath the thyroid cartilage and encircled the neck twice horizontally; the two ends of the cable overlapped, forming a cross-over point in the front-right of the neck. Then, the ligature passed obliquely through the nape, gradually disappearing, forming a gap in the mark. The mark was sharply defined, stiff, yellow, and parchment-like. The investigators performed a three-dimensional scene reconstruction using the Trimble X7 Laser Scanner and the PC-Crash Multibody System. Even though the geometry of the ligature mark in the present case raised doubts about the manner of death, the three-dimensional reconstruction confirmed that the hanging was feasible without any external intervention.
Experimental Study on the Cross-Scale Relationship of Cemented Backfill under the Action of an Air-Entraining Agent
Authors: Liu Xiaosheng , Yang Dongjie , Wang Weijun
Published: November 2023

Air-entraining agents have the function of optimizing pores and improving the performance of backfill. In this study, we used tailings and cement as the main raw materials and added different amounts of air-entraining agents to make backfill samples. By testing the uniaxial compressive strength (UCS) and microstructure, macro- and micro characteristics were studied. Nuclear magnetic resonance technology was used to explore pore characteristics, and fractal theory was used to quantitatively discuss the complexity of pore structure. Finally, a cross-scale relationship model between UCS and pores was established. The main conclusions are as follows: (1) Adding the appropriate amount of air-entraining agents can optimize pore structure and increase the UCS of backfill materials, which is beneficial to backfill materials. (2) The pores of backfill materials have fractal characteristics, the fractal effects of pores with different pore size ranges are different, and the air-entraining agent has a certain influence on the fractal characteristics of the pores. (3) There are inverse relationships between UCS and different pore size ranges.
Dilatancy Equation Based on the Property-Dependent Plastic Potential Theory for Geomaterials
Authors: Li Xuefeng , Zhu Houying , Yuan Qi
Published: November 2023

The dilatancy equation ignores the noncoaxiality of granular soil for the coaxial assumption of the direction of the stress and strain rate in conventional plastic potential theory, which is inconsistent with extensive laboratory tests. To reasonably describe the noncoaxial effects on dilatancy, the energy dissipation of plastic flow is derived based on the property-dependent plastic potential theory for geomaterials and integrates the noncoaxiality, the potential theory links the plastic strain of granular materials with its fabric, and the noncoaxiality is naturally related to the mesoscopic properties of materials. When the fabric is isotropic, the dilatancy equation degenerates into the form of the critical state theory, and when the fabric is anisotropic, it naturally describes the effects of noncoaxiality. In the plane stress state, a comparison between a simple shear test and prediction of the dilatancy equation shows that the equation can reasonably describe the effect of noncoaxiality on dilatancy with the introduction of microscopic fabric parameters, and its physical significance is clear. This paper can provide a reference for the theoretical description of the macro and micro mechanical properties of geomaterials.
Effect of Shear History on Solid–Liquid Transition of Particulate Gel Fuels
Authors: Li Jian , Li Yaning , Xiao Wei , Wang Jingyan , Wang Boliang
Published: November 2023

Investigating the structural evolution of particulate gels is a very challenging task due to their vulnerability and true flow characteristics. In this work, deeper insight into the rheological properties of gel fuels filled with fumed silica (FS) and aluminum microparticles (Al MPs) was gained by changing shear procedures. Firstly, the flow curves were found to no longer follow the monotonic power law and exhibited subtle thixotropic responses. As the shear rate increased, the gel structure underwent a transition from local shear to bulk shear in the nonlinear region after yielding. This finding reveals the prevalence of nonideal local shear in industry. Secondly, the time-dependent rheological responses demonstrated that the strength spectrum of gel fuels depends on the applied shear rate, with stress relaxation more easily observed at lower shear rates. Those results involved the structural disruption, recovery, and equilibrium of particulate gels from two scales of shear rate and shear time.
Tri-Component Hydrogel as Template for Nanocrystalline Hydroxyapatite Deposition Using Alternate Soaking Method for Bone Tissue Engineering Applications
Authors: Pelin Irina Mihaela , Popescu Irina , Calin Manuela , Rebleanu Daniela , Voicu Geanina , Ionita Daniela , Zaharia Marius-Mihai , Constantin Marieta , Fundueanu Gheorghe
Published: November 2023

Composite hydrogels containing apatite-like particles can act as scaffolds for osteoblast proliferation, with applications in bone tissue engineering. In this respect, porous biocompatible hydrogels were obtained from chitosan, oxidized pullulan, and PVA in different ratios. The stability of the hydrogels was ensured both by covalent bonds between aldehyde groups of oxidized pullulan and free amino groups of chitosan, and by physical bonds formed during freeze–thaw cycles and lyophilization. The deposition of calcium phosphates was performed by alternate soaking of the porous hydrogels into solutions with calcium and phosphate ions, assuring a basic pH required for hydroxyapatite formation. The mineralized hydrogels were characterized using FTIR spectroscopy, scanning electron microscopy, X-ray diffraction, and thermogravimetric analysis, showing that inorganic particles containing between 80 and 92% hydroxyapatite were deposited in a high amount on the pore walls of the polymeric matrix. The composition of the organic matrix influenced the crystallization of calcium phosphates and the mechanical properties of the composite hydrogels. In vitro biological tests showed that mineralized hydrogels support the proliferation of MG-63 osteoblast-like cells to a greater extent compared to pristine hydrogels.
Superior Conductivity of Transparent ZnO/MoS2 Composite Films for Optoelectronic and Solar Cell Applications
Authors: Tareq Shahad , Kirkil Gokhan , Özuğur Uysal Bengü
Published: November 2023

The use of transparent conductive oxides in optoelectronics created a revolution where new-generation materials with high transmittance, low sheet resistance values, durability, and portability can be achieved without decreasing efficiency or increasing costs. Transparent ZnO/MoS2 sandwich-structured conductive composite films were produced in this study via the sol–gel method, which is considered the most efficient method due to its simple process and low cost. The crystal structure properties of ZnO/MoS2 were characterized via X-ray diffraction (XRD) patterns. The crystal sizes of ZnO films doped with different amounts of MoS2 were determined. A UV–visible absorption spectrometer was used to perform the spectroscopic analysis of the film. The area under the absorption curve and the full width of the half-maxima of absorbance data were calculated. Using these values, the optimum amount of MoS2 was determined for the best additive distribution. In addition, in order to determine the best transparent conductive material, resistance values measured via the four-point probe method were compared for different MoS2 additive amounts. The optical and electrical characterizations of transparent ZnO/MoS2 conductive oxide films were investigated. According to the parameters obtained via UV–vis spectroscopy, XRD, and four-point probe measurements, the most effective dispersion that exhibits a low width ratio and high resonance ratio was found for ZnO/MoS2 with a doping amount of 4 mg, the crystallite size of the films was found to be within the range of 21.5 and 24.6 nm, and these observations demonstrated a figure-of-merit value of more than 4.8 × 10−2 with respect to these sandwich-structured films. Compared to the values of previous studies on various transparent ZnO-doped conductive oxide materials, it is possible to claim that these new films have a structure that is very similar to the transparent conductivity characteristics of other films, and they may even be superior relative to some MoS2 amounts.
Modeling Dynamics of Laterally Impacted Piles in Gravel Using Erosion Method
Authors: Yosef Tewodros Y. , Faller Ronald K. , Fang Chen , Kim Seunghee
Published: November 2023

Understanding the dynamic interaction between piles and the surrounding soil under vehicular impacts is essential for effectively designing and optimizing soil-embedded vehicle barrier systems. The complex behavior of pile–soil systems under impact loading, attributed to the soil’s nonlinear behavior and large deformation experienced by both components, presents significant simulation challenges. Popular computation techniques, such as the updated Lagrangian finite element method (UL-FEM), encounter difficulties in scenarios marked by large soil deformation, e.g., impacts involving rigid piles. While mesh-free particle and discrete element methods offer another option, their computational demands for field-scale pile–soil impact simulations are considerable. We introduce the erosion method to bridge this gap by integrating UL-FEM with an erosion algorithm for simulating large soil deformations during vehicular impacts. Validation against established physical impact tests confirmed the method’s effectiveness for flexible and rigid pile failure mechanisms. Additionally, this method was used to examine the effects of soil mesh density, soil domain sizes, and boundary conditions on the dynamic impact response of pile–soil systems. Our findings provide guidelines for optimal soil domain size, mesh density, and boundary conditions. This investigation sets the stage for improved, computationally efficient techniques for the pile–soil impact problem, leading to better pile designs for vehicular impacts.
Unlocking Resilience: How Physical Literacy Impacts Psychological Well-Being among Quarantined Researchers
Authors: Meng Han , Tang Xin , Qiao Jun , Wang Huiru
Published: November 2023

This study investigates the interplay between physical literacy, resilience, and burnout among researchers who experienced strict home quarantine during the COVID-19 pandemic in China, with a particular focus on the mediating role of resilience. Employing a two-stage sampling procedure, a total of 363 researchers from diverse disciplines, notably social science and natural science, were enlisted and administered a series of validated scales, including the Perceived Physical Literacy Scale (PPL), the 10-item Connor–Davidson Resilience Scale (CD-RISC-10), and the Chinese version of the Oldenburg Burnout Inventory (OLBL), via an anonymous online platform. The findings revealed substantial differences in physical literacy, resilience, and burnout across disciplines. Resilience partially mediated the relationship between physical literacy and burnout. Upon a closer examination of the sub-dimensions, resilience was found to fully mediate between factors of motivation, exhaustion, and disengagement. Moreover, one aspect of physical literacy—interaction with the environment—exhibited weaker correlations with both resilience and burnout compared to other dimensions of physical literacy. Overall, the study confirms the significant correlation between physical literacy and psychological parameters, establishing that elevated levels of both physical literacy and resilience serve as key factors in mitigating burnout during the pandemic.
Composite Angioimmunoblastic T-Cell Lymphoma and Diffuse Large B-Cell Lymphoma Presenting with Distributive Shock
Authors: Hariharan Nisha , Kabadi Alisha , Don Michelle , Odish Mazen , Heyman Benjamin
Published: November 2023

Diffuse large B-cell lymphoma (DLBCL) and angioimmunoblastic T-cell lymphoma (AITL) are two subtypes of non-Hodgkin lymphoma (NHL). The simultaneous occurrence of DLBCL and AITL in a composite lymphoma is very rare, and there are no established treatment regimens. We present the case of an 85-year-old male admitted to the intensive care unit with distributive shock, lymphocytosis, and lymphadenopathy, who was subsequently diagnosed with composite AITL and DLBCL, and treated with brentuximab vedotin (BV) and rituximab. To our knowledge, this is the first case of composite lymphoma presenting with distributive shock and treated with BV and rituximab, with successful resolution of shock.
Effect of the Ozone Application in the Nutrient Solution and the Yield and Oxidative Stress of Hydroponic Baby Red Chard
Authors: Machuca Vargas Alejandra , Silveira Gómez Ana Cecilia , Hernández-Adasme Cristian , Escalona Contreras Víctor Hugo
Published: November 2023

Novel ozone (O3) sanitizing treatments can be used to decrease the microbial load during cultivation, but they would affect the composition of the nutrient solution. Variations in the nutrient composition decrease crop yields, especially if a strong oxidizing agent such as ozone is used. In this study, O3 was applied throughout the culture every two days at doses of 0.0 (control); 0.5; 1.0; and 2.0 mg·L−1 for 3 min on baby red chard (Beta vulgaris L. cv. SCR 107) grown in a floating hydroponic system. Macronutrients and micronutrients in the nutrient solution, yield, antioxidant compounds, and oxidative stress enzymes were evaluated in plants. Macronutrients in the nutrient solution were not affected by O3, whereas micronutrients, such as Fe and Mn, decreased by 88.2 and 39.6%, respectively, at the 0.5 mg·L−1 dose. The dose of 0.5 mg·L−1 produced more fresh matter and leaf area than the control. Antioxidant capacity and total phenols were not significantly affected by O3 treatments; however, higher SOD, CAT, and APX activity after O3 applications were found. It is concluded that ozone applications to the nutrient solution affect the availability of some micronutrients and increase oxidative stress and yield in baby red chard plants.
Theoretical Analysis and Experimental Research on the Apple Auto-Orientation Based on Flexible Roller
Authors: Luo Tongyun , Zhou Jianguo , Zhang Shuo , Chen Jun , Hu Guangrui , Sugirbay Adilet
Published: November 2023

After automatic in-field picking, apple stem shortening requires fixing the apple position and maintaining a relatively stable posture, which puts high demands on the automatic apple-orienting structure. In this paper, a novel dual roller compact apple field orientation structure with dual rollers rotating in the same direction is proposed. It can realize the uniform orientation of apples after automatic picking in any attitude, and the apple auto-orientation phenomenon is theoretically analyzed based on the accurately established apple model, then the apple orientation test platform was set up and a monocular camera combined with YOLOv5m was used to determine the time of apple orientation. The results showed that 70.21% and 96.81% of the apples were respectively oriented within 7 s and 28 s with only two flexible rollers rotating in the corresponding direction. All the apples were oriented, and 95.24% of them moved along the axis toward the calyx end. The generalizability of the apple orientation device for different shapes of apples was then verified, and the relationship between the shape characteristics of apples and orientation speed was later illustrated. A structural basis was finally presented for automatic stem shortening and surface damage detection in the apple field.
Improves the Resilience of Cucumber Seedlings under High-Light Stress through End-of-Day Addition of a Low Intensity of a Single Light Quality
Authors: Li Xue , Zhao Shiwen , Qiu Chun , Cao Qianqian , Xu Peng , Zhang Guanzhi , Wu Yongjun , Yang Zhenchao
Published: November 2023

In order to investigate whether an end-of-day (EOD) addition of a single light quality could help alleviate high-light stress in a cucumber, cucumber seedlings were subjected to a 9 d period of high-light stress (light intensity was 1300 ± 50 μmol·m−2·s−1) when they were growing to 3 leaves and 1 heart, while the red light (R), blue light (B), green light (G), far-red light (FR), and ultraviolet A (UVA) light were added in the end-of-day period. The present study was conducted to measure antioxidants, chlorophyll content, and its synthetic degradative enzymes and chlorophyll a fluorescence in response to the degree of stress in cucumber seedlings. The experimental results demonstrated that the addition of blue light, UVA light, and green light significantly decreased the SOD and POD activities in the middle of the treatment (6th day) compared to the dark (D) treatment and improved the absorption performance of the PSI reaction centre of the cucumber seedling leaves to a certain extent (PIABS), but the PSII capacity capture ability (TRo/RC) of the three treatments decreased compared to the D treatment. The MDA content of all the treatments had a significant decrease compared to that of the D treatment. The MDA content of all the treatments was significantly lower than that of D, and its FV/FM was increased to different degrees; the chlorophyll degrading enzyme PPH activity was significantly lower than that of the D treatment when a single light quality was added at the EOD period on the 9th d of treatment. In conclusion, cucumber seedlings subjected to short-term high-light stress can be added during the EOD period with a low-light intensity of a single R, G, B, or UVA light.
The Effect of Melasma on the Quality of Life in People with Darker Skin Types Living in Durban, South Africa
Authors: Mpofana Nomakhosi , Paulse Michael , Gqaleni Nceba , Makgobole Mokgadi Ursula , Pillay Pavitra , Hussein Ahmed , Dlova Ncoza Cordelia
Published: November 2023

Melasma is a common skin disorder of acquired hyperpigmentation that appears commonly on the face. Although asymptomatic, melasma causes psychosocial and emotional distress. This study aimed to assess melasma’s severity on people with darker skin types, evaluate the effects of melasma on the quality of life (QoL), and establish QoL predictors in affected individuals. This was a cross-sectional analytic study that enrolled 150 patients from three private dermatology clinics in Durban, South Africa who were diagnosed with melasma. The severity of melasma alongside QoL were measured using a melasma area and severity index (MASI) score and melasma quality of life scale (MELASQoL), respectively. The associations among factors and QoL were explored using multivariable methods and stepwise regression analysis. p-values less than 0.05 were considered significant. Enrolled patients were predominantly females (95%), of which 76% were of black African ethnicity, 9% were of Indian ethnicity, and 15% had mixed ancestry, with an average age of 47.30 years. Family history revealed that 61% had no prior melasma cases, while 39% had affected relatives, most commonly mothers (41%). The cheeks were the most common site for melasma. MASI score of Masi (β = 0.209, t = 2.628, p < 0.001), the involvement of cheeks (β = −0.268, t = −3.405, p < 0.001), level of education (β = −0.159, t = −2.029, p = 0.044), and being menopausal (β = −0.161, t = −2.027, p = 0.045) were found to be predictors of QoL. A regression model was created to forecast MELASQoL using these four predictors. This equation’s significance lies in its ability to enable the remote assessment of MELASQoL based on these four variables. It offers a valuable tool for researchers and medical professionals to quantitatively and objectively evaluate the impact of melasma on an individual’s quality of life.
Epigenetic Landscape and Therapeutic Implication of Gene Isoforms of Doublecortin-Like Kinase 1 for Cancer Stem Cells
Authors: Moore Landon L. , Houchen Courtney W.
Published: November 2023

While significant strides have been made in understanding cancer biology, the enhancement in patient survival is limited, underscoring the urgency for innovative strategies. Epigenetic modifications characterized by hereditary shifts in gene expression without changes to the DNA sequence play a critical role in producing alternative gene isoforms. When these processes go awry, they influence cancer onset, growth, spread, and cancer stemness. In this review, we delve into the epigenetic and isoform nuances of the protein kinase, doublecortin-like kinase 1 (DCLK1). Recognized as a hallmark of tumor stemness, DCLK1 plays a pivotal role in tumorigenesis, and DCLK1 isoforms, shaped by alternative promoter usage and splicing, can reveal potential therapeutic touchpoints. Our discussion centers on recent findings pertaining to the specific functions of DCLK1 isoforms and the prevailing understanding of its epigenetic regulation via its two distinct promoters. It is noteworthy that all DCLK1 isoforms retain their kinase domain, suggesting that their unique functionalities arise from non-kinase mechanisms. Consequently, our research has pivoted to drugs that specifically influence the epigenetic generation of these DCLK1 isoforms. We posit that a combined therapeutic approach, harnessing both the epigenetic regulators of specific DCLK1 isoforms and DCLK1-targeted drugs, may prove more effective than therapies that solely target DCLK1.
Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER Morphology
Authors: Cardoso Tatiana , Sampaio Miguel , Neves João , Oliveira Sofia , Moura Inês , Séneca Ana , Pissarra José , Pereira Susana , Pereira Cláudia
Published: November 2023

The endomembrane system in plant cells enables the cell to manage and coordinate a variety of membranous compartments so that they and their contents arrive at the right location. The secretory pathway is an essential part of this complex network and has its gateway at the Endoplasmic Reticulum. Therefore, alterations at the ER can affect how protein trafficking takes place and how cargo leaves this organelle. With this work, we assessed how abnormalities at the Endoplasmic Reticulum would interfere with protein sorting and trafficking. We used an Arabidopsis mutant—leb-2 GFP-h, presenting abnormal ER morphology, and evaluated the expression of aspartic proteinases and genes related to vacuolar transport along with the localization of a specific vacuolar sorting signal—plant-specific insert (PSI). Our results show that alterations in the leb-2 GFP-h mutant did not disrupt the transport of PSI–mCherry to the vacuole but influenced the expression of endogenous aspartic proteinases. Furthermore, the study of key endomembrane genes expression revealed an upregulation of the SNARE proteins AtVAMP722 and AtVAMP723. The leb-2 mutant seems not to interfere with vacuolar routes but may be implicated in secretion events.
Evaluating the Phytochemical Composition and Antioxidant Activity of Leaves of Different Rose Varieties
Authors: Sharma Shivani , Awasthi Lipakshi , Kumari Poonam
Published: November 2023

Rose is a commercially significant floricultural crop that has been used for various industrial as well as decoration purposes. Along with the beautification of rose flowers, their leaves are enriched with different biologically active compounds having various therapeutic uses. The current study was performed on the phytochemical and antioxidant activity of aqueous extracts of rose leaves. In our study, we found there are consequential variations observed in all the parameters, viz., total chlorophyll, carotenoids, total anthocyanin, total phenol, flavonoids, and antioxidant activity, among all the varieties. Among the ten varieties, Thelma Barlow revealed the maximum phenolic content (35.19 mg/g FW), flavonoids content (15.97 mg/g FW), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of IC50 value (206.86 ± 0.49 µg/mL), and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (301.62 ± 2.31 µg/mL). The variety Grand Amore presented the highest values for total chlorophyll (1.41 mg/g FW) and carotenoids (36.29 mg/g FW) content compared to other varieties. Also, a comparative correlation was studied amongst the phytochemicals such as anthocyanin content, total phenolic content, flavonoids, and antioxidant activities. Amongst the different rose varieties, Grand Amore and Thelma Barlow contain higher antioxidant potential, owing to their greater phytochemical activity. From our findings, we collectively concluded that fresh rose leaves contain potentially higher phenolic and flavonoid content, i.e., are responsible for higher antioxidant activity, which can be utilized for various pharmacological as well as food industries.
Field Test of the Propheromones of the Whitemarked Tussock Moth (WMTM) Orgyia leucostigma (J.E. Smith) (Lepidoptera: Erebidae)
Authors: Mayo Peter , Abeysekera Sumudu Deepa , Silk Peter J. , MaGee David I. , Leclair Gaetan , Sweeney Jon , Ogden Jeffrey
Published: November 2023

The whitemarked tussock moth (WMTM), Orgyia leucostigma (J.E. Smith) (Lepidoptera: Erebidae), is an economic pest in Nova Scotia, Canada, where it undergoes periodic outbreaks defoliating several tree species of economic value, including balsam fir, Abies balsamea (L.) Miller (Pinaceae). Herein is described a releasing device for the WMTM sex pheromone (Z,Z)-6, 9-heneicosadien-11-one based on a rubber septum, which converts pheromone precursors, such as acetals, namely (Z,Z)-11,11-dimethoxy-6,9-heneicosadiene and (Z,Z)-6,9-heneicosadien-11-one ethylene ketal, to the pheromone itself by the action of acetic acid and releases it over time. The pheromone is unstable in nature and, consequently, lures made with this compound will only attract WMTM for a day or two. The two pheromone precursors, however, are more stable, and are converted slowly into the pheromone by acetic acid impregnated in the releasing device, or by acidic conditions in the environment. The two pheromone precursors were synthesized in 2019 using a modified, previously published approach. Field trapping studies conducted from 2019–22 showed that traps baited with rubber septa loaded with either (Z,Z)-11,11-dimethoxy-6,9-heneicosadiene by itself or (Z,Z)-6,9-heneicosadien-11-one ethylene ketal plus acetic acid consistently caught significantly more WMTM than traps baited with blank septa in most experiments.
Red Blood Cell Exchange as a Valid Therapeutic Approach for Pregnancy Management in Sickle Cell Disease: Three Explicative Cases and Systematic Review of Literature
Authors: Valentini Caterina Giovanna , Pellegrino Claudio , Ceglie Sara , Arena Vincenzo , Di Landro Francesca , Chiusolo Patrizia , Teofili Luciana
Published: November 2023

Pregnancy in women with sickle cell disease (SCD) is a high-risk situation, especially during the third trimester of gestation and in the post-partum period, due to chronic hypoxia and vaso-occlusive phenomena occurring in the maternal–fetal microcirculation: as a result, unfavorable outcomes, such as intra-uterine growth restriction, prematurity or fetal loss are more frequent in SCD pregnancies. Therefore, there is a consensus on the need for a strict and multidisciplinary follow-up within specialized structures. Transfusion support remains the mainstay of treatment of SCD pregnancies, whereas more targeted modalities are still controversial: the benefit of prophylactic management, either by simple transfusions or by automated red blood cell exchange (aRBCX), is not unanimously recognized. We illustrate the cases of three SCD pregnant patients who underwent aRBCX procedures at our institution in different clinical scenarios. Moreover, we carried out a careful literature revision to investigate the management of pregnancy in SCD, with a particular focus on the viability of aRBCX. Our experience and the current literature support the use of aRBCX in pregnancy as a feasible and safe procedure, provided that specialized equipment and an experienced apheresis team is available. However, further research in this high-risk population, with appropriately powered prospective trials, is desirable to refine the indications and timing of aRBCX and to confirm the advantages of this approach on other transfusion modalities.
Fetal Renal Duplicated Collecting System at 14–16 Weeks of Gestation
Authors: Khatib Nizar , Bronshtein Moshe , Bachar Gal , Beloosesky Ron , Ginsberg Yuval , Zmora Osnat , Weiner Zeev , Gover Ayala
Published: November 2023

(1) Background: To examine the incidence of the prenatal diagnosis of the renal double-collecting system (rDCS) and describe its clinical outcome and associated genetic abnormalities. (2) Methods: This retrospective study included women who attended the obstetric clinic for early fetal anatomic sonography with findings of a renal DCS. Diagnosis was conducted by an expert sonographer using defined criteria. (3) Results: In total, 29,268 women underwent early ultrasound anatomical screening at 14–16 weeks, and 383 cases of rDCS were diagnosed (prevalence: 1:76). Associated abnormalities were diagnosed in eleven pregnancies; four had chromosomal aberrations. No chromosomal abnormalities were reported in isolated cases. Ectopic uretrocele and dysplastic kidney were diagnosed in 6 (1.5%) and 5 (1.3%) fetuses, respectively. One girl was diagnosed with vesicoureteral reflux and recurrent UTIs, and two boys were diagnosed with undescended testis. The recurrence rate of rDCS was 8% in subsequent pregnancies. (4) Conclusions: In light of its benign nature, we speculate that isolated rDCS may be considered a benign anatomic variant, but a repeat examination in the third trimester is recommended to assess hydronephrosis.
The Management of Coronary Artery Disease in TAVR Patients
Authors: Faroux Laurent , Villecourt Aurélien , Metz Damien
Published: November 2023

About half of the transcatheter aortic valve replacement (TAVR) recipients exhibit some degree of coronary artery disease (CAD), and controversial results have been reported regarding the impact of the presence and severity of CAD on clinical outcomes post-TAVR. In addition to coronary angiography, promising data has been recently reported on the use of both cardiac computed tomography angiography and the functional invasive assessment of coronary lesions whether by FFR or iFR in the work-up pre-TAVR. Despite mitigated available data, percutaneous revascularization of significant coronary lesions has been the routine strategy in TAVR candidates with CAD. Additionally, scarce data exists on the incidence, characteristics and management of coronary events post-TAVR, and increasing interest exists on the potential coronary access challenges in patients requiring coronary angiography/intervention post-TAVR. This review provides an updated overview of the knowledge of CAD in TAVR recipients, focusing on its prevalence, clinical impact, pre- and post-procedural evaluation and management.