bluuebunny/crossref_metadata_2025 huggingface dataset:
The perception of fungi among Karajá indigenous children and adolescents from Brazil
Authors: dos Reis Mazulkieliche Jeronimo , Leonardo-Silva Lucas , Xavier-Santos Solange
Published: February 2024

Background

Environmental perception involves the interpretation and interaction of individuals with their surroundings, influenced by cultural, social, and individual factors. Analyzing the environmental perception of children and adolescents contributes to fostering awareness and ethical behavior toward the environment. Indigenous communities, such as the Karajá from Brazil, possess significant environmental knowledge due to their connection with nature, providing distinctive insights into biodiversity and natural interconnections. In this study, the perception of fungi among Karajá indigenous children and adolescents was investigated.

Methods

The study involved 229 elementary school students from the Macaúba, Fontoura and Santa Isabel do Morro communities, located on Bananal Island, Brazil. Students were encouraged to draw what they knew about fungi and answer where they learned about fungi and what name they give to these organisms. The drawings were analyzed considering seven categories.

Results

The term most used to refer to fungi was hedoro(u) (56%), followed by fungus (21%) and mushroom (11%). Most students said they had learned about fungi in nature (38%) and at school (36%). The most represented organisms were in fact fungi (93%), mainly being portrayed in nature (94%). Most participants did not attribute any ecological function to fungi (83%), although 16% of them recognized fungi as decomposers and 1% as phytopathogenic agents. Negative aspects, particularly food contamination, were more frequently represented (13%) than positive aspects (4%). The drawings identified two morphological types: mushrooms (87%) and mold (13%). Among these mushrooms, 68% possibly represent the Amanita muscaria species.

Conclusions

Although the children and adolescents showed that they noticed the fungi around them, the group’s concept and understanding were limited to the figure of the mushroom and the negative aspects related to food contamination. Strong association of the fungi with the A. muscaria is noteworthy, since it does not occur in the environment in which the participants live, suggesting that external stimuli, such as TV or the internet, can influence their perception more than the nature they are exposed to.
Non-mitotic proliferation of malignant cancer cells revealed through live-cell imaging of primary and cell-line cultures
Authors: Tyagi Iram Shazia , Tsui Ho Yin Calvin , Chen Si , Li Xinyi , Mat Wai-Kin , Khan Muhammad A. , Choy Lucas Brendan , Chan Ka-Yin Aden , Chan Tat-Ming Danny , Ng Chi-Ping Stephanie , Ng Ho-Keung , Poon Wai Sang , Xue Hong
Published: February 2024

Introduction

Anti-mitosis has been a key strategy of anti-cancer therapies, targeting at a fundamental property of cancer cells, their non-controllable proliferation due to overactive mitotic divisions. For improved anti-cancer therapies, it is important to find out whether cancer cells can proliferate independent of mitosis and become resistant to anti-mitotic agents.

Results

In this study, live-cell imaging was applied to both primary-cultures of tumor cells, and immortalized cancer cell lines, to detect aberrant proliferations. Cells isolated from various malignant tumors, such as Grade-III hemangiopericytoma, atypical meningioma, and metastatic brain tumor exhibit distinct cellular behaviors, including amoeboid sequestration, tailing, tunneling, nucleic DNA leakage, as well as prokaryote-like division such as binary fission and budding-shedding, which are collectively referred to and reported as ‘non-mitotic proliferation’ in this study. In contrast, benign tumors including Grade-I hemangiopericytoma and meningioma were not obvious in such behaviors. Moreover, when cultured in medium free of any anti-cancer drugs, cells from a recurrent Grade-III hemangiopericytoma that had been subjected to pre-operation adjuvant chemotherapy gradually shifted from non-mitotic proliferation to abnormal mitosis in the form of daughter number variation (DNV) and endomitosis, and eventually regular mitosis. Similarly, when treated with the anti-cancer drugs Epirubicin or Cisplatin, the cancer cell lines HeLa and A549 showed a shift from regular mitosis to abnormal mitosis, and further to non-mitosis as the dominant mode of proliferation with increasing drug concentrations. Upon removal of the drugs, the cells reversed back to regular mitosis with only minor occurrences of abnormal mitosis, accompanied by increased expression of the stem cell markers ALDH1, Sox, Oct4 and Nanog.

Conclusions

The present study revealed that various types of malignant, but not benign, cancer cells exhibited cellular behaviors indicative of non-mitotic proliferation such as binary fission, which was typical of prokaryotic cell division, suggesting cell level atavism. Moreover, reversible transitions through the three modes of proliferation, i.e., mitosis, abnormal mitosis and non-mitosis, were observed when anticancer drug concentrations were grossly increased inducing non-mitosis or decreased favoring mitosis. Potential clinical significance of non-mitotic proliferation in cancer drug resistance and recurrence, and its relationship with cancer stem cells are worthy of further studies.
Determinants of survival following heart transplantation in adults with congenital heart disease
Authors: Sicim Hüseyin , Noly Pierre Emmanuel , Naik Suyash , Sood Vikram , Ohye Richard G. , Haft Jonathan W. , Aaronson Keith D. , Pagani Francis D. , Si Ming-Sing , Tang Paul C.
Published: February 2024

Background

Adult patients surviving with congenital heart disease (ACHD) is growing. We examine the factors associated with heart transplant outcomes in this challenging population with complex anatomy requiring redo-surgeries.

Methods

We reviewed the United Network for Organ Sharing-Standard Transplant Analysis and Research database and analyzed 35,952 heart transplants from January 1st, 2000, to September 30th, 2018. We compared transplant characteristics for ischemic cardiomyopathy (ICM) (n = 14,236), nonischemic cardiomyopathy (NICM) (n = 20,676), and ACHD (n = 1040). Mean follow-up was 6.20 ± 4.84 years. Kaplan–Meier survival curves and Cox-proportional hazards analysis were used to analyze survival data.

Results

Multivariable analysis confirmed that ACHD was associated greater in-hospital death compared to ICM (HR = 0.54, P < 0.001) and NICM (HR = 0.46, P < 0.001). Notable factors associated with increased mortality were history of cerebrovascular disease (HR = 1.11, P = 0.026), prior history of malignancy (HR = 1.12, P = 0.006), pre-transplant biventricular support (HR = 1.12, P = 0.069), postoperative stroke (HR = 1.47, P < 0.001) and postoperative dialysis (HR = 1.71, P < 0.001). ACHD transplants had a longer donor heart ischemic time (P < 0.001) and trend towards more deaths from primary graft dysfunction (P = 0.07). In-hospital deaths were more likely with ACHD and use of mechanical support such as use of right ventricular assist device (HR = 2.20, P = 0.049), biventricular support (HR = 1.62, P < 0.001) and extracorporeal membrane oxygenation (HR = 2.36, P < 0.001). Conditional survival after censoring hospital deaths was significantly higher in ACHD (P < 0.001).

Conclusion

Heart transplant in ACHD is associated with a higher post-operative mortality given anatomical complexity but a better long-term conditional survival. Normothermic donor heart perfusion may improve outcomes in the ACHD population by reducing the impact of longer ischemic times.
Hybrid operating room with ceiling mounted imaging system assisted pre-operative and intra-operative lung nodule localization for thoracoscopic resections: a 5-year case series
Authors: Chia Audrey Qi Xin , Gogna Apoorva , Pena Angela Maria Takano , Sai Vishnu Vemula Sri , Chandramohan Sivanathan , Chan Shaun Ju Min Xavier , Ong Boon-Hean
Published: February 2024

Background

Video-assisted thoracoscopic (VATS) lung resections are increasingly popular and localization techniques are necessary to aid resection. We describe our experience with hybrid operating room (OR) cone-beam computed tomography (CT) assisted pre-operative and intra-operative lesion localization of lung nodules for VATS wedge resections, including our novel workflow using the hybrid OR cone-beam CT to re-evaluate patients who have undergone pre-operative localization for those who are unsuitable for intra-operative localization.

Methods

Retrospective analysis of all consecutive patients with small (≤ 20 mm), deep (≥ 10 mm distance from pleura) and/or predominantly ground-glass nodules selected for lesion localization in the Interventional Radiology suite followed by re-evaluation with cone-beam CT in the hybrid OR (pre-operative), or in the hybrid OR alone (intra-operative), prior to intentional VATS wedge performed by a single surgeon at our centre from January 2017 to December 2021.

Results

30 patients with 36 nodules underwent localization. All nodules were successfully resected with a VATS wedge resection, although 10% of localizations had hookwire or coil dislodgement. The median effective radiation dose in the pre-operative group was 10.4 mSV including a median additional radiation exposure of 0.9 mSV in the hybrid OR for reconfirmation of hookwire or coil position prior to surgery (p = 0.87). The median effective radiation dose in the intra-operative group was 3.2 mSV with a higher mean rank than the intra-operative group, suggesting a higher radiation dose (p = 0.01).

Conclusions

We demonstrate that our multidisciplinary approach utilizing the hybrid OR is safe and effective. Intra-operative localization is associated with lower radiation doses. Routine use of cone-beam CT to confirm the position of the physical marker prior to surgery in the hybrid OR helps mitigate consequences of localization failure with only a modest increase in radiation exposure.
Needs of amyloidosis patients and their care providers: design & first results of the AMY-NEEDS research and care program
Authors: Ihne-Schubert Sandra Michaela , Radovic Teresa , Fries Saskia , Frantz Stefan , Einsele Hermann , Störk Stefan , Neuderth Silke
Published: February 2024

Background

Amyloidosis represents a rare yet heterogeneous multi-system disorder associated with a grave prognosis and an enormous psycho-emotional strain on patients, relatives, and caregivers. We here present the overall study design and first results of AMY-NEEDS, a research program aiming to systematically assess the needs of patients suffering from amyloidosis, their relatives and health care professionals (HCPs), and develop an amyloidosis-specific care approach.

Methods

AMY-NEEDS uses a mixed-methods approach including focus groups (step 1), a questionnaire-based broad evaluation within the local amyloidosis patient collective (step 2), and the development of a needs-adapted care concept (step 3).

Results

Seven patients, six relatives and five HCPs participated in the focus groups (step 1). At the time of diagnosis, patients expressed the need of a smooth diagnostic process, possibly enhanced through improved awareness and better education of local HCPs. There was a strong wish to receive well-founded information and comprehensive support including companionship during medical visits, experience the feeling of being understood, find trust in that “everything possible” is being done, and have effortless access to centre staff. In the course of the disease, patients favoured that the specialized centre should manage treatment coordination, monitoring and psychosocial support. The interface between centre and local HCPs was regarded of particular importance, requiring further investigation into its optimal design.

Conclusions

Patients with amyloidosis express particular needs that should appropriately be considered in specifically tailored care concepts.
Performance and clinical utility of a new supervised machine-learning pipeline in detecting rare ciliopathy patients based on deep phenotyping from electronic health records and semantic similarity
Authors: Faviez Carole , Vincent Marc , Garcelon Nicolas , Boyer Olivia , Knebelmann Bertrand , Heidet Laurence , Saunier Sophie , Chen Xiaoyi , Burgun Anita
Published: February 2024

Background

Rare diseases affect approximately 400 million people worldwide. Many of them suffer from delayed diagnosis. Among them, NPHP1-related renal ciliopathies need to be diagnosed as early as possible as potential treatments have been recently investigated with promising results. Our objective was to develop a supervised machine learning pipeline for the detection of NPHP1 ciliopathy patients from a large number of nephrology patients using electronic health records (EHRs).

Methods and results

We designed a pipeline combining a phenotyping module re-using unstructured EHR data, a semantic similarity module to address the phenotype dependence, a feature selection step to deal with high dimensionality, an undersampling step to address the class imbalance, and a classification step with multiple train-test split for the small number of rare cases. The pipeline was applied to thirty NPHP1 patients and 7231 controls and achieved good performances (sensitivity 86% with specificity 90%). A qualitative review of the EHRs of 40 misclassified controls showed that 25% had phenotypes belonging to the ciliopathy spectrum, which demonstrates the ability of our system to detect patients with similar conditions.

Conclusions

Our pipeline reached very encouraging performance scores for pre-diagnosing ciliopathy patients. The identified patients could then undergo genetic testing. The same data-driven approach can be adapted to other rare diseases facing underdiagnosis challenges.
Identification of copy number variations among fetuses with isolated ultrasound soft markers in pregnant women not of advanced maternal age
Authors: Liu Yunyun , Liu Sha , Liu Jianlong , Bai Ting , Jing Xiaosha , Deng Cechuan , Xia Tianyu , Cheng Jing , Xing Lingling , Wei Xiang , Luo Yuan , Zhou Quanfang , Xie Dan , Xiong Yueyue , Liu Ling , Zhu Qian , Liu Hongqian
Published: February 2024

Background

Pathogenic (P) copy number variants (CNVs) may be associated with second-trimester ultrasound soft markers (USMs), and noninvasive prenatal screening (NIPS) can enable interrogate the entire fetal genome to screening of fetal CNVs. This study evaluated the clinical application of NIPS for detecting CNVs among fetuses with USMs in pregnant women not of advanced maternal age (AMA).

Results

Fetal aneuploidies and CNVs were identified in 6647 pregnant women using the Berry Genomics NIPS algorithm.Those with positive NIPS results underwent amniocentesis for prenatal diagnosis. The NIPS and prenatal diagnosis results were analyzed and compared among different USMs. A total of 96 pregnancies were scored positive for fetal chromosome anomalies, comprising 37 aneuploidies and 59 CNVs. Positive predictive values (PPVs) for trisomy 21, trisomy 18, trisomy 13, and sex chromosome aneuploidies were 66.67%, 80.00%, 0%, and 30.43%, respectively. NIPS sensitivity for aneuploidies was 100%. For CNVs, the PPVs were calculated as 35.59% and false positive rate of 0.57%. There were six P CNVs, two successfully identified by NIPS and four missed, of which three were below the NIPS resolution limit and one false negative. The incidence of aneuploidies was significantly higher in fetuses with absent or hypoplastic nasal bone, while that of P CNVs was significantly higher in fetuses with aberrant right subclavian artery (ARSA), compared with other groups.

Conclusions

NIPS yielded a moderate PPV for CNVs in non-AMA pregnant women with fetal USM. However, NIPS showed limited ability in identifying P CNVs. Positive NIPS results for CNVs emphasize the need for further prenatal diagnosis. We do not recommend the use of NIPS for CNVs screening in non-AMA pregnant women with fetal USM, especially in fetuses with ARSA.
Barriers to gene therapy, understanding the concerns people with haemophilia have: an exigency sub-study
Authors: Fletcher Simon , Jenner Kathryn , Holland Michael , Khair Kate
Published: February 2024

Background

Gene therapy has the potential to offer people with haemophilia (PwH) a life free from bleeding and the burden posed by current treatment regimens. To date, gene therapy has only been available in clinical trial settings, to PwH without pre-existing or historical factor inhibitors, significant concomitant liver damage or pre-existing neutralising antibodies to the adeno-associated viruses used to deliver the therapy. Thus, most PwH treated at centres not currently involved in gene therapy trials, either as a referral/follow-up centre or as a dosing centre, have been unable to access the therapy. This Exigency sub-study aims to gain a greater understanding of the opinions of PwH in the United Kingdom who have not had access to gene therapy: asking what they understand, what concerns they have, and whether they perceive any barriers preventing their access to gene therapy.

Results

Twenty-three PwH were approached; 14 consented, and one withdrew prior to interview. The mean age of the participants was 35.7 years (range 25–74 years). Eleven had haemophilia A and two haemophilia B. Two were treated with standard half-life factor products, five with extended half-life products, five with a FVIII mimetic and one with a clinical trial product. One family member (a participant's partner) was also interviewed. The participants identified four barriers to gene therapy: concerns about the process of gene therapy (Expectations), uncertainty about the results (outcomes), (Access) to treatment, and a lack of understanding about gene therapy (education).

Conclusions

This Exigency study subgroup sees gene therapy as a positive treatment development that promises an improved quality of life. For this participant group, four issues impact their decision to undergo gene therapy. If the promise of gene therapy is to be realised, these barriers need to be acknowledged and addressed by healthcare professionals, patient organisations, and gene therapy providers.
Clinical symptoms, biochemistry, and liver histology during the native liver period of progressive familial intrahepatic cholestasis type 2
Authors: Kondou Hiroki , Nakano Satoshi , Mizuno Tadahaya , Bessho Kazuhiko , Hasegawa Yasuhiro , Nakazawa Atsuko , Tanikawa Ken , Azuma Yoshihiro , Okamoto Tatsuya , Inui Ayano , Imagawa Kazuo , Kasahara Mureo , Zen Yoh , Suzuki Mitsuyoshi , Hayashi Hisamitsu
Published: February 2024

Background

Progressive familial intrahepatic cholestasis type 2 (PFIC2) is an ultra-rare disease caused by mutations in the ABCB11 gene. This study aimed to understand the course of PFIC2 during the native liver period.

Methods

From November 2014 to October 2015, a survey to identify PFIC2 patients was conducted in 207 hospitals registered with the Japanese Society of Pediatric Gastroenterology, Hepatology, and Nutrition. Investigators retrospectively collected clinical data at each facility in November 2018 using pre-specified forms.

Results

Based on the biallelic pathogenic variants in ABCB11 and/or no hepatic immunohistochemical detection of BSEP, 14 Japanese PFIC2 patients were enrolled at seven facilities. The median follow-up was 63.2 [47.7–123.3] months. The median age of disease onset was 2.5 [1–4] months. Twelve patients underwent living donor liver transplantation (LDLT), with a median age at LDLT of 9 [4–57] months. Two other patients received sodium 4-phenylbutyrate (NaPB) therapy and survived over 60 months with the native liver. No patients received biliary diversion. The cases that resulted in LDLT had gradually deteriorated growth retardation, biochemical tests, and liver histology since the initial visit. In the other two patients, jaundice, growth retardation, and most of the biochemical tests improved after NaPB therapy was started, but pruritus and liver fibrosis did not.

Conclusions

Japanese PFIC2 patients had gradually worsening clinical findings since the initial visit, resulting in LDLT during infancy. NaPB therapy improved jaundice and growth retardation but was insufficient to treat pruritus and liver fibrosis.
Zero Self-Harm app: a mobile phone application to reduce non-suicidal self-injury—study protocol for a randomized controlled trial
Authors: Guerrero Evelyn , Andreasson Kate , Larsen Lene , Buus Niels , Skovgaard Larsen Jette Louise , Krogh Jesper , Thastum Rasmus , Lindberg Lone , Lindblad Katrine , Erlangsen Annette , Nordentoft Merete
Published: February 2024

Background

Non-suicidal self-injury (NSSI) is a growing healthcare problem. Individuals with NSSI have an increased risk of suicidality. Due to stigma, they may self-injure in secret, which means they might not seek help until events have escalated to include suicidal ideation or a mental disorder. Interventions delivered via mobile phone applications (apps) have been linked to reductions in self-injury. This protocol outlines a trial, which examines whether the Zero Self-Harm intervention, consisting of an app for people with NSSI, can reduce the number of NSSI episodes, suicide ideation, and depressive symptoms.

Methods

The trial will be conducted as a 6-month 2-arm, parallel-group, multicentre, pragmatic, randomized clinical superiority trial. The intervention group will receive the app and instructions on how to use it, while the control group will be allocated to a waitlist and allowed to download the app after 6 months. After inclusion, participants will be asked to complete questionnaires at baseline, 3 months, and 6 months. The primary outcome is the number of NSSI episodes during the preceding month, as measured at the 6 months follow-up with the Deliberate Self-Harm Inventory. A total of 280 participants, 140 in each arm, will be included.

Discussion

This trial will assess the effectiveness of the Zero Self-Harm intervention to reduce the number of NSSI episodes. If effective, the app will have the potential to support a large group of people with NSSI. Considering the stigma related to NSSI, the fact that the app may be used in private and anonymously might make it an appealing and acceptable option for support. The app was developed in collaboration with people with lived experiences related to current and/or previous NSSI. As a result of this, the app focuses on minimizing harm, rather than stopping NSSI. This might enhance its utilization.

Trial registration

ClinicalTrials.gov NCT04463654. Registered on 7 June 2020.
Good Statistical Practice—development of tailored Good Clinical Practice training for statisticians
Authors: Stocken Deborah D. , Mossop Helen , Armstrong Emma , Lewis Steff , Dutton Susan J. , Peckitt Claire , Gamble Carrol , Brown Julia
Published: February 2024

Background

Statisticians are fundamental in ensuring clinical research, including clinical trials, are conducted with quality, transparency, reproducibility and integrity. Good Clinical Practice (GCP) is an international quality standard for the conduct of clinical trials research. Statisticians are required to undertake training on GCP but existing training is generic and, crucially, does not cover statistical activities. This results in statisticians undertaking training mostly unrelated to their role and variation in awareness and implementation of relevant regulatory requirements with regards to statistical conduct. The need for role-relevant training is recognised by the UK NHS Health Research Authority and the Medicines and Healthcare products Regulatory Agency (MHRA).

Methods

The Good Statistical Practice (GCP for Statisticians) project was instigated by the UK Clinical Research Collaboration (UKCRC) Registered Clinical Trials Unit (CTU) Statisticians Operational Group and funded by the National Institute for Health and Care Research (NIHR), to develop materials to enable role-specific GCP training tailored to statisticians. Review of current GCP training was undertaken by survey. Development of training materials were based on MHRA GCP. Critical review and piloting was conducted with UKCRC CTU and NIHR researchers with comment from MHRA. Final review was conducted through the UKCRC CTU Statistics group.

Results

The survey confirmed the need and desire for the development of dedicated GCP training for statisticians. An accessible, comprehensive, piloted training package was developed tailored to statisticians working in clinical research, particularly the clinical trials arena. The training materials cover legislation and guidance for best practice across all clinical trial processes with statistical involvement, including exercises and real-life scenarios to bridge the gap between theory and practice. Comprehensive feedback was incorporated. The training materials are freely available for national and international adoption.

Conclusion

All research staff should have training in GCP yet the training undertaken by most academic statisticians does not cover activities related to their role. The Good Statistical Practice (GCP for Statisticians) project has developed and extensively piloted new, role-specific, comprehensive, accessible GCP training tailored to statisticians working in clinical research, particularly the clinical trials arena. This role-specific training will encourage best practice, leading to transparent and reproducible statistical activity, as required by regulatory authorities and funders.
Effectiveness of structured psycho-oncological counseling for relatives of lung cancer patients based on the CALM approach—study protocol of a randomized controlled trial
Authors: Dusel Julia , Meng Karin , Arnold Hanna , Rabe Antonia , Jentschke Elisabeth
Published: February 2024

Background

The high incidence combined with the high lethality and bad prognosis of lung cancer highlight the need for psycho-oncological care for both patients and their relatives. While psychological interventions for relatives might be helpful, further research on the impact of specific interventions is necessary. Therefore, this trial aims to evaluate structured psycho-oncological counseling for relatives of lung cancer patients based on the Managing Cancer And Living Meaningfully (CALM) approach compared to usual care. In addition, we explore the impact of psycho-oncological support of relatives on the patients’ mental health outcomes.

Methods

The study is a single-center, prospective, randomized controlled trial with two measurement time points. Relatives of lung cancer patients and, thus, the patients themselves (i.e., dyads) are randomly allocated to the intervention group (IG) or the control group (CG) regardless of their disease or treatment stage. Relatives in the IG receive structured counseling based on the CALM approach (three to six sessions with psycho-oncologists). The CG receives usual psycho-oncological care. In addition, cancer patients in both study arms can request psycho-oncological support (usual care) as needed, but they will not get a specific intervention. Relatives and patients complete assessments at baseline (T0) and after the intervention/6 weeks (T1). The primary outcome for relatives is anxiety. Relatives’ secondary outcomes include depressive symptoms, distress, supportive care needs, and quality of life. Patients’ outcomes include anxiety, depression, and distress. All outcomes are assessed using self-report validated questionnaires. Intervention effects will be evaluated using analysis of covariance (ANCOVA) adjusting for baseline values. Power calculations reveal the need to enroll 200 subjects to detect an effect of d = 0.4.

Discussion

The study will provide evidence for the effectiveness of the CALM intervention in relatives of lung cancer patients. Furthermore, study results will contribute to a better understanding of the effectiveness of a psycho-oncological intervention for highly impaired cancer patients and their relatives. If the CALM intervention positively affects the relatives’ psychosocial outcome, it may be implemented in routine care.

Trial registration

German Clinical Trials Register DRKS00030077. Retrospectively registered on 26 October 2022.
Rudi Kundini, Pamoja Kundini (RKPK): study protocol for a hybrid type 1 randomized effectiveness-implementation trial using data science and economic incentive strategies to strengthen the continuity of care among people living with HIV in Tanzania
Authors: Kadota Jillian L. , Packel Laura J. , Mlowe Matilda , Ulenga Nzovu , Mwenda Natalino , Njau Prosper F. , Dow William H. , Wang Jingshen , Sabasaba Amon , McCoy Sandra I.
Published: February 2024

Background

Economic incentives can improve clinical outcomes among in-care people living with HIV (PLHIV), but evidence is limited for their effectiveness among out-of-care PLHIV or those at risk of disengagement. We propose a type 1 hybrid effectiveness-implementation study to advance global knowledge about the use of economic incentives to strengthen the continuity of HIV care and accelerate global goals for HIV epidemic control.

Methods

The Rudi Kundini, Pamoja Kundini study will evaluate two implementation models of an economic incentive strategy for supporting two groups of PLHIV in Tanzania. Phase 1 of the study consists of a two-arm, cluster randomized trial across 32 health facilities to assess the effectiveness of a home visit plus one-time economic incentive on the proportion of out-of-care PLHIV with viral load suppression (< 1000 copies/ml) 6 months after enrollment (n = 640). Phase 2 is an individual 1:1 randomized controlled trial designed to determine the effectiveness of a short-term counseling and economic incentive program offered to in-care PLHIV who are predicted through machine learning to be at risk of disengaging from care on the outcome of viral load suppression at 12 months (n = 692). The program includes up to three incentives conditional upon visit attendance coupled with adapted counselling sessions for this population of PLHIV. Consistent with a hybrid effectiveness-implementation study design, phase 3 is a mixed methods evaluation to explore barriers and facilitators to strategy implementation in phases 1 and 2. Results will be used to guide optimization and scale-up of the incentive strategies, if effective, to the larger population of Tanzanian PLHIV who struggle with continuity of HIV care.

Discussion

Innovative strategies that recognize the dynamic process of lifelong retention in HIV care are urgently needed. Strategies such as conditional economic incentives are a simple and effective method for improving many health outcomes, including those on the HIV continuum. If coupled with other supportive services such as home visits (phase 1) or with tailored counselling (phase 2), economic incentives have the potential to strengthen engagement among the subpopulation of PLHIV who struggle with retention in care and could help to close the gap towards reaching global “95–95-95” goals for ending the AIDS epidemic.

Trial registration

Phase 1: ClinicalTrials.gov, NCT05248100, registered 2/21/2022.

Phase 2: ClinicalTrials.gov, NCT05373095, registered 5/13/2022.
Gut microbiome-metabolome interactions predict host condition
Authors: Shtossel Oshrit , Koren Omry , Shai Iris , Rinott Ehud , Louzoun Yoram
Published: February 2024

Background

The effect of microbes on their human host is often mediated through changes in metabolite concentrations. As such, multiple tools have been proposed to predict metabolite concentrations from microbial taxa frequencies. Such tools typically fail to capture the dependence of the microbiome-metabolite relation on the environment.

Results

We propose to treat the microbiome-metabolome relation as the equilibrium of a complex interaction and to relate the host condition to a latent representation of the interaction between the log concentration of the metabolome and the log frequencies of the microbiome. We develop LOCATE (Latent variables Of miCrobiome And meTabolites rElations), a machine learning tool to predict the metabolite concentration from the microbiome composition and produce a latent representation of the interaction. This representation is then used to predict the host condition.

LOCATE’s accuracy in predicting the metabolome is higher than all current predictors. The metabolite concentration prediction accuracy significantly decreases cross datasets, and cross conditions, especially in 16S data.

LOCATE’s latent representation predicts the host condition better than either the microbiome or the metabolome. This representation is strongly correlated with host demographics. A significant improvement in accuracy (0.793 vs. 0.724 average accuracy) is obtained even with a small number of metabolite samples ($$\sim 50$$

∼
50

).

Conclusion

These results suggest that a latent representation of the microbiome-metabolome interaction leads to a better association with the host condition than any of the two separated or the simple combination of the two.
Exploring the Mediterranean tsunami research landscape: scientometric insights and future prospects
Authors: Laksono F x Anjar Tri , Mishra Manoranjan , Mulyana Budi , Kovács János
Published: February 2024

Background

The Mediterranean Sea is a region characterized by high seismic activity, with at least 200 tsunami events recorded from the fourth century to the present twenty-first century. Numerous studies have been conducted to understand past tsunami events, earthquake–tsunami generation, tsunami recurrence periods, tsunami vulnerability zones, and tsunami hazard mitigation strategies. Therefore, gaining insights into future trends and opportunities in Mediterranean Sea tsunami research is crucial for significantly contributing to all relevant aspects. This study aims to assess such trends and opportunities through a scientometric analysis of publications indexed by Web of Science from 2000 to 2023.

Results

Based on a selection of 329 publications, including research articles, review articles, book chapters, and conference papers, published between 2000 and 2023, Italy has the highest number of publications and citations in this field. The number of publications has increased significantly, especially after the 2004 Indian Ocean, 2011 Tohoku, and 2018 Palu tsunamis. According to the keyword analysis, the terms “tsunami”, “earthquake”, “hazard”, “wave”, “Mediterranean”, “coast”, and “tectonic” were the most frequently used in these publications. Research themes consist of four classifications: motor themes, such as seismic hazard; specific but well-developed themes, like tsunamiite; emerging or disappearing themes, for example, climate change; and general or basic themes, such as equations and megaturbidite. The number of publications related to the motor theme classification continued to grow throughout 2000–2023. Topics from 2011–2023 are more complex compared to 2000–2010, characterized by the emergence of new keywords such as evacuation planning, risk reduction, risk mitigation, building vulnerability, coastal vulnerability, climate change, probabilistic tsunami hazard assessment (PTVA-3 and PTVA-4). However, topics that were popular in the 2000–2010 period (e.g., paleotsunami deposits, earthquake, and tsunami propagation analysis) also increased in 2011–2023.

Conclusions

Research topics with high centrality and density such as seismic hazard will continue to develop and prospect. The cluster network of this topic includes seismoturbidites, sedimentary features, tsunami modeling, active faults, catalog, and historical earthquakes.
An automatic method using MFCC features for sleep stage classification
Authors: Pei Wei , Li Yan , Wen Peng , Yang Fuwen , Ji Xiaopeng
Published: February 2024

Sleep stage classification is a necessary step for diagnosing sleep disorders. Generally, experts use traditional methods based on every 30 seconds (s) of the biological signals, such as electrooculograms (EOGs), electrocardiograms (ECGs), electromyograms (EMGs), and electroencephalograms (EEGs), to classify sleep stages. Recently, various state-of-the-art approaches based on a deep learning model have been demonstrated to have efficient and accurate outcomes in sleep stage classification. In this paper, a novel deep convolutional neural network (CNN) combined with a long short-time memory (LSTM) model is proposed for sleep scoring tasks. A key frequency domain feature named Mel-frequency Cepstral Coefficient (MFCC) is extracted from EEG and EMG signals. The proposed method can learn features from frequency domains on different bio-signal channels. It firstly extracts the MFCC features from multi-channel signals, and then inputs them to several convolutional layers and an LSTM layer. Secondly, the learned representations are fed to a fully connected layer and a softmax classifier for sleep stage classification. The experiments are conducted on two widely used sleep datasets, Sleep Heart Health Study (SHHS) and Vincent’s University Hospital/University College Dublin Sleep Apnoea (UCDDB) to test the effectiveness of the method. The results of this study indicate that the model can perform well in the classification of sleep stages using the features of the 2-dimensional (2D) MFCC feature. The advantage of using the feature is that it can be used to input a two-dimensional data stream, which can be used to retain information about each sleep stage. Using 2D data streams can reduce the time it takes to retrieve the data from the one-dimensional stream. Another advantage of this method is that it eliminates the need for deep layers, which can help improve the performance of the model. For instance, by reducing the number of layers, our seven layers of the model structure takes around 400 s to train and test 100 subjects in the SHHS1 dataset. Its best accuracy and Cohen’s kappa are 82.35% and 0.75 for the SHHS dataset, and 73.07% and 0.63 for the UCDDB dataset, respectively.
Discussing the gaps in the science and practice of lived experience engagement in mental health and substance use research: results of knowledge mobilization activities
Authors: Hawke Lisa D. , Rockburne Faith , Hiebert Melissa , Putterman Connie , Sheikhan Natasha Y.
Published: February 2024

Background

Engaging people with lived experience of mental health or substance use challenges and family members (PWLE) improves the quality and relevance of the associated research, but it can be challenging to include them meaningfully and authentically in the work.

Knowledge mobilization events

After reviewing the literature on the science of lived experience engagement, we held two knowledge mobilization events to translate the findings to relevant partners and collect their feedback to guide our future research. A total of 55 people attended, bringing the perspective of people with lived experience, family members, research staff, research trainees, and scientists, as well as attendees holding multiple roles. We presented the scoping review findings, then held discussions to solicit feedback and encourage the sharing of perspectives.

Attendee perspectives

Through small and large group discussion activities, we found that our scoping review findings resonated with the attendees’ personal experiences with engagement in mental health and substance use research. Among the gaps highlighted in the discussions, the two that were most emphasized were the critical importance of improving diversity in engagement work in mental health and substance use, and the importance of addressing gaps around communication, relationships, rapport, and power dynamics in engagement spaces.

Conclusions

Diversity, communication, relationships, and power dynamics emerge as key areas of work needed in the near future to advance the science of PWLE engagement in mental health and substance use research. We commit to pursuing the work that is considered of greatest need by a range of partners this research engagement sphere. We call on researchers in this area to continue this line of work, with a focus on the areas of research identified by attendees.
Analgesic effect of neuromodulation using the AT-04 portable magnetic field-generating device in a patient with neuropathic pain: a case report
Authors: Sawada Atsushi , Yamakage Michiaki
Published: February 2024

Background

Neuromodulation by magnetic field through the AT-04 (ait® (AT-04); Peace of Mind Co., Ltd., Kumamoto, Japan) has improved allodynia in neuropathic pain model rats. This report focuses on neuromodulation through magnetic field exposure using the AT-04 that provided an analgesic effect in a patient with neuropathic pain.

Case presentation

A 47-year-old man presented with flaccid paralysis and extensive neuropathic pain and scored 7 on the 11-point Numerical Rating Scale (NRS) for his left upper limb. The patient was treated with neuromodulation by magnetic field exposure using the AT-04. Baseline NRS scores were obtained three times daily during the baseline period (days 1–5). Magnetic field exposure was then performed for 30 min three times daily (morning, noon, and evening) at home for 36 days, which was termed the intervention period (days 6–41). During the baseline period, the median NRS score was 7 and the baseline NRS score for calculating the percentage of nonoverlap data (PND) was 6. During the intervention period, the median NRS score was 4 and the PND value of the NRS score was 77.8% (28/36). Neuromodulation by magnetic field exposure using the AT-04 effectively decreased the patient’s NRS score. The patient had no adverse effects during the intervention period.

Conclusions

Neuromodulation by magnetic field exposure using the AT-04 was effective in decreasing the NRS score in a patient with neuropathic pain. The AT-04 portable magnetic field-generating device shows potential as a therapeutic option for refractory neuropathic pain.
Experimental analysis for optimization of process parameters in machining using coated tools
Authors: Mohanta Dillip Kumar , Sahoo Bidyadhar , Mohanty Ardhendu Mouli
Published: February 2024

Manufacturers are facing challenges in achieving high productivity and quality in manufacturing through machining. PVD-coated tools can control several machining challenges by enhancing hardness and abrasion resistance of the cutting tool. These tools facilitate turning operations in terms of efficiency, accuracy, and productivity by extending cutting performance and tool life. Aluminum bronze, a copper alloy valued for its mechanical, thermal, corrosion, and wear-resistant properties, finds application in diverse industries such as aerospace, automobile, marine, and electrical engineering, as well as in the creation of sculptures, decorative elements, and thermal devices. However, machining aluminum bronze presents common challenges, including achieving a smooth surface finish and minimizing high cutting force due to its inherent strength and abrasiveness. This research aims at identifying the optimal levels of cutting velocity, feed, and depth of cut to minimize surface roughness and cutting force during dry turning of wear-resistant high-strength CuAl10Fe5Ni5-C. PVD AlTiN-coated tools were utilized, which offer many advantages over others. Experiments were conducted through Taguchi’s L27 OA (orthogonal array) of factors. The results indicate that coated tools have superior performance in reducing surface roughness and cutting force. When it comes to designing and optimizing experiments, integrating PCA with Taguchi method is a potent strategy. Again, it was observed that feed is the most influential factor affecting responses.
Tectonic analysis of the Mula River Basin, Kirthar fold belt, Pakistan, using hypsometric index
Authors: Hussain Ammar , Afzal Amna , Khan Shuhab D.
Published: February 2024

The Mula River Basin is in an active tectonic region of the Kirthar fold fault belt in the Western Himalayas. The presence of numerous major faults like Chaman Fault, Kirthar Frontal Fault, and Bannh Fault near the study suggests complex tectonic processes in the region. The seismic record of the study area also indicates that this area is tectonically active. This makes the study area an ideal site to measure tectonic activity through geomorphic indices like hypsometric integrals (HI). For tectonic analysis of the Mula River Basin, we divided the study area into 309 subbasins. The results obtained from the HI calculations for the subbasins led us to the classification of the study area into three classes, i.e., class 1 (0.51–0.78), class 2 (0.37–0.50), and class 3 ([Formula: see text]), where class 1 is for the highest tectonic activity, class 2 responds to moderate, and class 3 is for the lowest tectonic activity. We calculated the hypsometric curves to understand the geomorphological cycle of the Mula River Basin. We quantified the subbasins of the Mula River Basin inside the highly active tectonic zone of Kirthar fold and fault zone as per their tectonic activity and found that a major portion of the study area indicates low tectonic activity (44.33%), medium tectonically active, and high tectonically active subbasins are 37.86% and 17.79%, respectively. These findings are supported by the presence of high-relief areas and known faults in the study area.
FEATURES OF FORMATION OF READINESS OF FUTURE TEACHERS TO BUILD A PROFESSIONAL CAREER IN CHANGING CONDITIONS
Authors: Bakhtiyarova Venera F. , Gabidullina Svetlana M.
Published: February 2024

Introduction. The professional activity of a teacher is multifunctional, and responsibility for their own professional success rests with the teacher himself. However, a significant part of the graduates of the pedagogical university is not ready enough to build their professional career.

The purpose of the work is to theoretically substantiate and describe the effectiveness of the implementation of pedagogical conditions that ensure the formation of the readiness of future teachers to build a professional career.

Methods and materials. Systematic, acmeological, activity-based, personality-oriented approaches are considered as the methodological basis of the author’s research. The solution of research tasks is provided by a set of complementary theoretical and empirical methods.

Results. The essence of the concept of readiness of future teachers to build a professional career is clarified. The criterion apparatus for determining the readiness of future teachers to build a professional career is presented. The effectiveness of pedagogical conditions for the formation of the readiness of future teachers to build a professional career has been experimentally proved. The research materials can be used in the system of secondary vocational and higher pedagogical education to form students – future teachers’ readiness to build a professional career.

Conclusion. The revealed pedagogical conditions contribute to the formation of the readiness of future teachers to build a professional career. The presented practical experience can be used in the construction of a professional training space for future teachers and in other educational organizations.
METHODS OF COMPOSITION TRAINING IN FORMATION OF AESTHETIC CULTURE OF ARTISTIC AND PEDAGOGICAL SPECIALTIES STUDENTS
Authors: Filimonyuk Lyudmila A. , Konoval Anastasia R.
Published: February 2024

Purpose. The article is devoted to the topic of the use of composition teaching methods in the process of forming the aesthetic culture of students, which is relevant in the conditions of modern art and pedagogical education. The subject of analysis is the methods of teaching composition in order to increase the level of aesthetic culture of students, future teachers of fine arts. The author aims to reveal the potential and role of composition teaching methods in the process of forming the aesthetic culture of students.

Method or methodology of the work. The study is based on a systematic approach that made it possible to consider the problem in the relationship between the methods of teaching composition and the process of forming the aesthetic culture of students. The study is based on methods of studying and analyzing the theoretical and methodological literature, which examine aspects of this problem, comparative analysis of the literature, as well as the systematization of the information obtained.

Results. The teaching methods applicable to composition classes in order to form the aesthetic culture of artistic and pedagogical specialties students are described.

Scope of the results. The results obtained can be applied in the practice of training future fine arts teachers.