bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving the Protective Properties of Paints and Varnishes by Optimizing the Coating System
Authors:   I.Ya. Yakovlev Chuvash State Pedagogical University, Fadeev I.V. , Uspenskiy I.A. ,   Ryazan State Agrotechnological University named after P.A.Kostychev, Stepanova E.I. ,   Ryazan State Agrotechnological University named after P.A.Kostychev, Khaylov N.I. ,   Ryazan State Agrotechnological University named after P.A.Kostychev
Published: September 2022

The physical and mechanical properties, moisture capacity, adhesion to the substrate of films of various compositions of paints and varnishes have been studied. It has been established that enamels applied over a layer of phosphating primers on the surface of metals exhibit the best protective properties. Coating systems with phosphating primers have high continuity, better physical and mechanical properties, and adhesion to the surface of the metal being protected from corrosion.
Analytical Assessment and Consideration of the Properties of Electromagnetic Fields in Agro-disinfection Devices
Authors:   Donskoy Agrarian Research Center, Pakhomov A.I.
Published: September 2022

Studies have been carried out to assess the properties of electromagnetic fields used in the disinfection of grain and seeds. Cause-and-effect relationships between the non-monochromaticity of the field and the shortcomings of microwave and other methods of disinfection have been established. For a better result, it is proposed to use monochromatic working fields, the advantages of which are demonstrated by the example of a new method of resonant low-frequency disinfection. An analysis of the electric and magnetic components of the field in the output device was performed, based on the results of which an effective technical solution based on the electric component was proposed. The superiority of a device with an electrical component over a solenoid has been experimentally confirmed.
تحديات عولمة الاعلام وسبل المواجهة
Authors: العزاوي مظفر مندوب
Published: September 2022

تمخضت عن المتغيرات الدولية المتلاحقة في الميادين الاقتصادية والسياسية والثقافية وغيرها العديد من الظواهر التي احتلت مواقع متباينة من الاهتمام والمتابعة والدراسة والتحليل من قبل المختصين والباحثين وخاصة في ظل التطورات الاعلامية المتسارعة في الخريطة الاتصالية العالمية.
فكانت هناك ظاهرة " تفجير المعلومات" المتمثلة في استخدام الحاسب الالكتروني في تخزين واسترجاع خلاصة ما انتجه الفكر البشري في أقل حيز متاح وباسرع وقت ممكن(1) والتي تعتبر " الانترنت " شبكة المعلومات الدولية من ابرز مظاهرها والتي اتسع نشاطها و تضاعف دورها المعلوماتي في عملية الاتصال الدولية في مختلف العلوم والفنون ثم انتشرت ظاهرة " البث الفضائي المباشر " التي جعلت العالم قرية صغيرة الكترونية كما قال عالم الاتصال الكندي " مارشال ماكلوهان " انما تحولت الى بيت صغير بل الى شاشة صغيرة.
ولكن رغم ما عكسته هذه الظواهر وغيرها في الميدان الاتصالي من تطور وتغيير في آليات التعامل بين المجتمعات الدولية لكنها افرزت في الوقت نفسه ظاهرة الاختلال في تصنيع وتوزيع وتوظيف المعلومات وتقنياتها كانت ارجحيتها لصالح بلدان لايتجاوز عددها اصابع اليد الواحدة مقابل اخرى يتجاوز عددها المائة والثمانون دولة .
كل هذه الظواهر مجتمعة وما يتصل بها معنى واسلوباً وهدفاً دخل في بودقة واحدة اطلق عليها " عولمة الاعلام " التي اتخذت لها صيغاً واسلحة مختلفة باتت تشكل تحدياً للبلدان التي ما تزال في وضع اعلامي لاتحسد عليه بسبب تواضع امكانياتها التقنية والبشرية مقابل بلدان تتفوق عليها بهذه الصناعة بكل خلفياتها واستخداماتها والاهداف المرسومة لها , وهنا تكمن عملية التحدي الاعلامي المتدفق من الخارج .
ويأتي بحثنا لتسليط الضوء على مايشكله هذا التحدي  من انعكاسات على البلدان التي تتعرض له من خلال الكشف عن الحلقات الغامضة التي تحيط بمفهوم العولمة من وجهة نظر عامة وعولمة الاعلام من وجه التحدد, هذه الظاهرة وافرازاتها قد اصبحت هاجس العصر ومنطلقاً للعديد من الافكار والدراسات والمعالجات التي تناولتها بمختلف مفاهيمها واتجاهاتها ووظائفها اضافة الى الكشف عن ما هو جديد في ميدان عولمة الاعلام وما تشكله من تحديات على البلدان المستهدفة منها , فقد اصبحت عولمة الاعلام باساليبها ووسائلها واهدافها تشكل باستخداماتها وانعكاساتها السلبية على وجه التحديد خطراً  يهدد الواقع الاقليمي والامني للبلدان التي تتعرض لمخاطرها وخاصة بلدان العالم الثالث التي تتدفق اليها من جانب واحد كميات هائلة من المعلومات والبرامج المتلفزة مصدرها بلدان الشمال المصنعة والمصدرة لها وهدفها بلدان الجنوب المستقبلة والمستهلكة لهما. الامر الذي يشكل اختلالاً في تبادل المعلومات نوعاً وكماً بين تلك البلدان لصالح البلدان المتطورة وهذا الاختلال بدوره اصبح يشكل وحده احدى مظاهر ومخاطر عولمة الاعلام في عصرنا الراهن على المجتمعات التي ما تزال في وضع لايسمح لها مواكبة ما يجري من تطورات تقنية في ميادين الاتصال, الهيمنة فيها لعدد محدد من البلدان المتطورة وخاصة من خلال البث الفضائي وما تمخض عنه من استحداث لمئات القنوات الفضائية الوافدة هذه المشكلة وتفاعلاتها ومعالجاتها ستخضع لمتطلبات هذا البحث ومن خلال المحاور الاتية:
At the sources of Ukrainian Juvenile Justice: Valery Levitsky and Yuriy Novitsky – founders of the Kiev court for minors
Authors: Samoilenko Olena
Published: September 2022

The idea of the birth of courts for children originated in Australia, but it was widely known in 1899 in the United States. The main idea was to replace the reprisals for the birth of children, as if they had committed a malice, a wrongful-vichalic beginning. To have good indications that the idea was transferred in 1904 to Western Europe – back to England, then to Germany, France and in 1910, as an experiment, this was implemented in Russia in the city of St. Petersburg. For the results of the activity of a special court for juveniles in St. Petersburg, it was ruled out that people would come to the court to order such a court in Kiev.
The process of organizing the court dragged on for a long time. Until the fi rst September of 1913, the project of splitting and making submissions to the Kiev Star of the World Judges. After that, with insignifi cant changes in the vins of fi lings to the 1st Department of the Ministry of Justice. In early 1913, the Ministry of Justice praised the project, and on September 7, 1914, the Kyiv City Court of the World Courts praised the courts of minors accused and minor victims in an open court. All right, they were seen and entrusted to the additonal justice of the peace. On September 7, 1914, the Kiev City Hall of the World Judgment made a decision to appoint a young, talented, accomplished justice of the peace, Valeriy Mikhailovich Levitsky, to the tenure.
The article deals with the life and creative path of outstanding but little – known lawyers, active members of the Society of Kiev patronage, founders of the Kiev court for minors-Valery Mikhailovich Levitsky and Yuri Petrovich Novitsky. A general overview of their offi cial careers, their role and place in the formation of the Ukrainian juvenile justice system is presented. Their steady energy in achieving their goals and
amazing hard work are shown.

Key words: Society Kiev patronage, Kiev court for minors, additional Justice of the peace, justice of the peace, minors.
FEATURES OF BANKRUPTCY IN SIMPLIFIED BANKRUPTCY PROCEDURES
Authors:   International Law Institute, Moscow, Russian Federation, PILCHINA DAMIRA
Published: September 2022

In this article, the author sets the goal of studying the specific characteristics of simplified bankruptcy procedures, within the framework of which the features of the legal regulation of bankruptcy proceedings in simplified bankruptcy procedures of special debtors are considered: a liquidated debtor, an absent debtor, as well as a specialized company and a mortgage agent. The Bankruptcy Law excludes the application of monitoring, financial rehabilitation and external management procedures in relation to these special debtors, aimed at assessing the debtor's financial condition and implementing rehabilitation measures to restore his solvency, which corresponds to the task of reducing the duration and resource intensity of simplified bankruptcy procedures. The specifics of bankruptcy proceedings in respect of absent and liquidated debtors are insignificant, which do not allow to separate simplified bankruptcy proceedings as a special procedure. Bankruptcy proceedings carried out in relation to a specialized company and a mortgage agent do not have a wide practical application due to the organizational «protection» of these legal entities from bankruptcy. The author concludes that the current legislative regulation of simplified bankruptcy procedures does not contain significant differences between simplified bankruptcy proceedings and ordinary competition, which is a significant drawback of the legal regulation of simplified bankruptcy. The author presents proposals to simplify the procedure for bankruptcy proceedings of absent and liquidated debtors, including the rejection of mandatory quarterly meetings of creditors and the transition to simplified reporting of the bankruptcy trustee, proposals related to reforming the procedure for the sale of the debtor's property, including by developing a separate procedure for the sale of property in simplified bankruptcy procedures, as well as simplification of the procedure for the sale of receivables arrears.
ISSUES OF LAW ENFORCEMENT IN INSURANCE RELATIONSHIPS: NEW TRENDS
Authors:   Institute of the state and right of the Russian Academy of Sciences, Moscow, Russian Federation, OVCHINNIKOVA YULIA
Published: September 2022

The article pays attention to the legal nature of insurance protection and insurance services. Some problems of law enforcement in insurance legal relations are considered, in particular, in liability insurance for damage compensation. It is noted that the insurance legislation contradicts some provisions of civil law. Attention is paid to some aspects of law enforcement when bringing an insurer to civil liability. Conclusions are drawn about the need to amend insurance legislation, as well as proposals for reforming insurance law.
Mukbang and Me: Implications on Cognition and Physical Well-Being among Undergraduates
Authors: Jenging Rosnnie , Mohamad Fitri Suraya
Published: September 2022

Mukbang has become a global eating trend, especially among youths. Mukbang is mainly prevalent on social media platforms and has become addictive content for many who follow such videos. This study explores the effects of watching mukbang on cognitive functions, health problems and social interaction patterns. The study uses neurofeedback to analyse the brainwaves of selected participants who are university-going youths and have a regular habit of watching Mukbang every day. Two situations were recorded using neurofeedback; the first is only watching Mukbang passively, while the other is where the participant watched Mukbang and ate something simultaneously. A beta and alpha brainwave data were chosen to be analysed. Findings showed that the beta brainwave was recorded the highest in watching mukbang passively while the alpha was the highest during watching and eating mukbang. The result shows that anxiety, excitement, and focus were associated with the highest beta waves, while optimal cognitive performance was related to the activation of the upper alpha wave. The analysis provides a perspective to understand the effect of Mukbang on youths, specifically those currently studying at the tertiary level. The study links how idle interest in Mukbang affects cognitive activities and potentially triggers bingeing, worsening the relationship with food. Future research should examine how Mukbang encourages the diet Mukbangers would be tempted to adopt as daily food intake and how it affects long-term cognitive development among university-going youths.
Implementasi Kontrol PID untuk Pengaturan Kecepatan Motor DC pada Proses Pengupasan Bawang Merah
Authors: Khalid Mohammad , Fathoni Fathoni , Murtono Ari
Published: September 2022

Kontrol Kecepatan Motor DC pada Pengupasan Kulit Bawang Merah
IMPLEMENTASI INVERTER 1 FASA GELOMBANG SINUS PADA MODUL AJAR PEMBANGKIT LISTRIK TENAGA SURYA
Authors: Karim Haries Fadhilah , Radianto Donny , Putri Ratna Ika
Published: September 2022

Alat ini merupakan sebuah modul pembelajaran pada bidang renewable energy yang memanfaatkan tenaga surya. Pada prakteknya, modul dioperasikan dengan cara mengarahkan cahaya lampu pada solar cell. Dengan posisi kemiringan cahaya lampu dan solar cell yang dapat diatur secara manual dengan sumbu arah yang berbeda. Hal tersebut dapat mempengaruhi hasil untuk output tegangan dan arus pada solar cell.Pada modul ini terdapat 2 buah solar cell yang dapat dirangkai secara seri ataupun paralel, yang output-nya dihubungkan dengan baterai. Guna mencegah terjadinya over charge, maka modul juga dilengkapi dengan automatic charging system. Baterai dapat dihubungkan dengan beberapa beban DC atau dapat juga menjadi input untuk rangkaian inverter sehingga dapat digunakan untuk menyalakan AC.
Sistem Kontrol Kualtas Udara dan Suhu Inkubator Anak Ayam Pedaging Dengan Metode PID Berbasis IoT (Internet of Things)
Authors: Hanifa Zakhia , Parastiwi Andriani , Komarudin Achmad
Published: September 2022

Abstract – The high demand for chicken meat has increased the number of small-scale and large-scale industrial chicken farms. The waste generated from the chicken farming business is chicken manure. Chicken manure can indeed be used as fertilizer and has been used for a long time. However, due to the decomposition of the dirt, it smells. The smell comes from the high content of ammonia gas (NH3). During the maintenance period, broilers require adequate ambient temperature and lighting according to age for optimal growth. From these problems, an incubator was created that can control air quality and temperature which can also be monitored via cellphone in an application. The air sensor will activate the fan to stabilize the air quality. The temperature sensor turns on the light when the temperature in the incubator is too low and activates the fan if the temperature is too high Keywords: Incubator, Ammonia, Sensor, Fan Intisari— Banyaknya permintaan terhadap daging ayam membuat meningkatnya peternakan ayam dari yang skala kecil maupun yang skala industri besar. Limbah yang dihasilkan dari usaha peterakan ayam diantaranya kotoran ayam. Kotoran ayam memang dapat dijadikan sebagai pupuk dan sudah dimanfaatkan sejak lama. Tetapi akibat dekomposisi dari kotoran tersebut mengakibatkan bau. Bau tersebut berasal dari kandungan gas amonia (NH3) yang tinggi. Dalam masa pemeliharaan, ayam pedaging memerlukan suhu lingkungan dan pencahayaan yang memadai sesuai umur untuk pertumbuhan yang optimal. Dari permasalahan tersebut diciptakanlah inkubator yang dapat mengontrol kualitas udara dan suhu yang juga dapat di monitor lewat handphone dalam sebuah aplikasi. Sensor udara akan mengaktifkan kipas untuk menstabilkan kualitas udara. sensor suhu menyalakan lampu apabila suhu yang ada didalam incubator terlalu rendah dan akan mengaktifkan kipas apabila suhu terlalu tinggi. Kata Kunci : Setrika Uap Boiler, PID, Arduino Uno, Sensor PT100
In silico discovery of biomarkers for the accurate and sensitive detection of Fusarium solani
Authors: Bakare Olalekan Olanrewaju , Gokul Arun , Jimoh Muhali Olaide , Klein Ashwil , Keyster Marshall
Published: September 2022

Fusarium solani is worrisome because it severely threatens the agricultural productivity of certain crops such as tomatoes and peas, causing the general decline, wilting, and root necrosis. It has also been implicated in the infection of the human eye cornea. It is believed that early detection of the fungus could save these crops from the destructive activities of the fungus through early biocontrol measures. Therefore, the present work aimed to build a sensitive model of novel anti-Fusarium solani antimicrobial peptides (AMPs) against the fungal cutinase 1 (CUT1) protein for early, sensitive and accurate detection. Fusarium solani CUT1 receptor protein 2D secondary structure, model validation, and functional motifs were predicted. Subsequently, anti-Fusarium solani AMPs were retrieved, and the HMMER in silico algorithm was used to construct a model of the AMPs. After their structure predictions, the interaction analysis was analyzed for the Fusarium solani CUT1 protein and the generated AMPs. The putative anti-Fusarium solani AMPs bound the CUT1 protein very tightly, with OOB4 having the highest binding energy potential for HDock. The pyDockWeb generated high electrostatic, desolvation, and low van der Waals energies for all the AMPs against CUT1 protein, with OOB1 having the most significant interaction. The results suggested the utilization of AMPs for the timely intervention, control, and management of these crops, as mentioned earlier, to improve their agricultural productivity and reduce their economic loss and the use of HMMER for constructing models for disease detection.
Band 3–mediated Plasmodium vivax invasion is associated with transcriptional variation in PvTRAg genes
Authors: De Meulenaere Katlijn , Prajapati Surendra Kumar , Villasis Elizabeth , Cuypers Bart , Kattenberg Johanna Helena , Kasian Bernadine , Laman Moses , Robinson Leanne J. , Gamboa Dionicia , Laukens Kris , Rosanas-Urgell Anna
Published: September 2022

ThePlasmodium vivaxreticulocyte invasion process is still poorly understood, with only a few receptor-ligand interactions identified to date. Individuals with the Southeast Asian ovalocytosis (SAO) phenotype have a deletion in the band 3 protein on the surface of erythrocytes, and are reported to have a lower incidence of clinicalP. vivaxmalaria. Based on this observation, band 3 has been put forward as a receptor forP. vivaxinvasion, although direct proof is still lacking. In this study, we combined functionalex vivoinvasion assays and transcriptome sequencing to uncover a band 3–mediated invasion pathway inP. vivaxand potential band 3 ligands. Invasion byP. vivaxfield isolates was 67%-71% lower in SAO reticulocytes compared with non-SAO reticulocytes. Reticulocyte invasion was decreased by 40% and 27%-31% when blocking with an anti-band 3 polyclonal antibody and a PvTRAg38 peptide, respectively. To identify new band 3 receptor candidates, we mRNA-sequenced schizont-stage isolates used in the invasion assays, and observed high transcriptional variability in multigene and invasion-related families. Transcriptomes of isolates with low or high dependency on band 3 for invasion were compared by differential expression analysis, which produced a list of band 3 ligand candidates with high representation ofPvTRAggenes. Ourex vivoinvasion assays have demonstrated that band 3 is aP. vivaxinvasion receptor and confirm previousin vitrostudies showing binding between PvTRAg38 and band 3, although the lower and variable inhibition levels observed suggest the involvement of other ligands. By coupling transcriptomes and invasion phenotypes from the same isolates, we identified a list of band 3 ligand candidates, of which the overrepresentedPvTRAggenes are the most promising for future research.
Rare brain and pulmonary abscesses caused by oral pathogens started with acute gastroenteritis diagnosed by metagenome next-generation sequencing: A case report and literature review
Authors: Chen Minhua , Lai Zhiyi , Cheng Mingjun , Liang Tianyu , Lin Zongbin
Published: September 2022

Odontogenic brain and pulmonary abscesses are extremely rare infectious diseases. It is mainly caused by the upward or downward transmission of local infection or blood-borne spread. In recent years, with the wide application of some novel testing methods in clinical practice, the diagnosis of unexplained infections such as odontogenic abscesses in different organs has gradually become clear. We report a case of a 21-year-old male who was healthy and had not received any oral treatment before onset. He started with acute gastroenteritis-related symptoms, then developed meningitis-related symptoms seven days later with septic shock. No obvious abscess lesions were found on head computed tomography (CT) at admission, and the etiology was not clear by routine examination, which was very easy to misdiagnose as a serious infection caused by intestinal pathogens. But odontogenic pathogens were found both in his blood and cerebrospinal fluid through metagenomic next-generation sequencing (mNGS) analysis. Subsequently, rechecked imaging examination displayed multiple brain and pulmonary abscesses. Finally, it was diagnosed as an odontogenic brain and pulmonary abscess. After an extremely lengthy anti-infection course (13 weeks of intravenous antibiotics plus 2 weeks of oral antibiotics) and surgery, the patient was improved and discharged from the hospital. From this case, we could see that the development of new diagnostic technologies such as mNGS plays an important role in the early and confirmed diagnosis of diseases previously difficult to diagnose such as odontogenic polymicrobial infections and ultimately helps to improve the prognosis of these patients.
Abnormal prostate microbiota composition is associated with experimental autoimmune prostatitis complicated with depression in rats
Authors: Liu Feng , Xu Xiaolin , Wang Zhong , Wu Peng
Published: September 2022

Background

Microbiota play essential roles in the pathogenesis of prostatitis and depression. However, the changes in prostate microbiota have not yet been explored in rats with prostatitis/depression. This study aimed to investigate the changes of prostate microbiota in rats with prostatitis/depression.

Methods

Rats with experimental autoimmune prostatitis (EAP) complicated with depression were constructed through injection of rat prostate antigen with immunoadjuvants followed by application of chronic unpredictable mild stress (CUMS). The rats were subjected to inflammatory factor detection and behavioral testing to confirm the establishment of the model. Subsequently, the prostate microbiota was assayed in the rats and compared by 16S rRNA gene sequencing.

Results

A rat model of EAP complicated with depression was established and confirmed by increases in IL-1β, IL-6, and TNF-α as well as the occurrence of depressive‐like behaviors. EAP/CUMS significantly altered the richness, evenness, and composition of prostate microbiota. Forty-six taxonomic biomarkers for prostate microbiota were enriched in rats with EAP/depression and exhibited statistically significant and biologically consistent differences. Metabolomics profiling revealed that EAP/depression was associated with reductive acetyl coenzyme A pathway, L-lysine fermentation to acetate and butanoate, protein N-glycosylation and purine nucleobases degradation I, which is regulated by DCE29, Nocardioes, Helicobacter and Dorea.

Conclusion

Findings from the study demonstrate the existence of abnormal prostate microbiota in EAP complicated with depression and may be helpful in the treatment of comorbid diseases of prostatitis and depression.
Unguided virtual-reality training can enhance the oral presentation skills of high-school students
Authors: Valls-Ratés Ïo , Niebuhr Oliver , Prieto Pilar
Published: September 2022

Public speaking is fundamental in our daily life, and it happens to be challenging for many people. Like all aspects of language, these skills should be encouraged early on in educational settings. However, the high number of students per class and the extensive curriculum both limit the possibilities of the training and, moreover, entail that students give short in-class presentations under great time pressure. Virtual Reality (VR) environments can help speakers and teachers meet these challenges and foster oral skills. This experimental study employs a between-subjects pre- and post-training design with four Catalan high-school student groups, a VR group (N = 30) and a Non-VR group (N = 20). Both groups gave a 2-min speech in front of a live audience before (pre-training) and after (post-training) 3 training sessions (one session per week) in which they practiced public speaking either in front of a VR audience or alone in a classroom (Non-VR). Students assessed their anxiety measures right before performing every speech and filled out a satisfaction questionnaire at the end. Pre- and post-training speeches were assessed by 15 raters, who analyzed the persuasiveness of the message and the charisma of the presenter. Speeches were also analyzed for prosodic features and gesture rate. First, results showed that self-assessed anxiety got significantly reduced at post-training for both conditions. Second, acoustic analyses of both groups' speeches show that the VR group has, unlike the Non-VR group, developed a more clear and resonant voice quality in the post-training speeches, in terms of higher cepstral-peak prominence (CPP) (although no significant differences in f0- related parameters as a function of training were obtained), as well as significantly less erosion effects than the Non-VR group. However, these differences across groups did not trigger a direct improvement on the participants' gesture rate, persuasiveness and charisma at post-training. Furthermore, students perceived the training in the VR environment to be more useful and beneficial for their future oral presentations than the Non-VR group. All in all, short unguided VR training sessions can help students feel less anxious, promote a more clear and resonant voice style, and can prevent them from experiencing an erosion effect while practicing speeches in front of a real audience.
Massive hemoptysis bridged with VV ECMO: A case report
Authors: Ryan Dylan , Miller Kathleen , Capaldi Carly , Pasquarello Claudine , Yang Qiong , Hirose Hitoshi
Published: September 2022

Objective

Extracorporeal membrane oxygenation (ECMO) can provide full pulmonary support when a patient is completely apneic. The combination of veno-venous (VV) ECMO and induced apnea can be utilized to control significant hemoptysis. We present a case of massive hemoptysis that developed while on VV ECMO and was treated with temporary discontinuation of the ventilator and serial declotting bronchoscopies.

Methods

A 42-year-old male with recent acute ST elevation myocardial infarction status post cardiac stent developed aspiration pneumonia that progressed to acute respiratory distress syndrome. The patient's biventricular function was preserved. VV ECMO was placed for lung rescue on hospital day #7, and tracheostomy was performed for ventilator dependence on hospital day #12. On hospital day #18, the patient developed significant hemoptysis despite the discontinuation of anticoagulation. Bronchoscopy revealed massive bleeding from bilateral bronchi. To facilitate tamponade within the tracheobronchial tree, the ventilator was temporarily discontinued while VV ECMO provided full respiratory support. After 48 h, mechanical ventilation was resumed, and daily bronchoscopies were performed to remove clots from both bronchi until a chest x-ray showed improvement in bilateral opacifications. Bronchoscopy was performed a total of 14 times. There was no recurrence of bronchial bleeding, the patient's respiratory status improved, and VV ECMO was weaned off on hospital day #37. The patient was transferred to a long-term rehabilitation facility 36 days after successful VV ECMO decannulation on hospital day #73.

Conclusions

This patient's survival of massive hemoptysis was facilitated largely by the utilization of serial declotting bronchoscopies with VV ECMO providing full pulmonary support during temporary discontinuation of mechanical ventilation.
Using machine learning to improve neutron identification in water Cherenkov detectors
Authors: Jamieson Blair , Stubbs Matt , Ramanna Sheela , Walker John , Prouse Nick , Akutsu Ryosuke , de Perio Patrick , Fedorko Wojciech
Published: September 2022

Water Cherenkov detectors like Super-Kamiokande, and the next generation Hyper-Kamiokande are adding gadolinium to their water to improve the detection of neutrons. By detecting neutrons in addition to the leptons in neutrino interactions, an improved separation between neutrino and anti-neutrinos, and reduced backgrounds for proton decay searches can be expected. The neutron signal itself is still small and can be confused with muon spallation and other background sources. In this paper, machine learning techniques are employed to optimize the neutron capture detection capability in the new intermediate water Cherenkov detector (IWCD) for Hyper-K. In particular, boosted decision tree (XGBoost), graph convolutional network (GCN), and dynamic graph convolutional neural network (DGCNN) models are developed and benchmarked against a statistical likelihood-based approach, achieving up to a 10% increase in classification accuracy. Characteristic features are also engineered from the datasets and analyzed using SHAP (SHapley Additive exPlanations) to provide insight into the pivotal factors influencing event type outcomes. The dataset used in this research consisted of roughly 1.6 million simulated particle gun events, divided nearly evenly between neutron capture and a background electron source. The current samples used for training are representative only, and more realistic samples will need to be made for the analyses of real data. The current class split is 50/50, but there is expected to be a difference between the classes in the real experiment, and one might consider using resampling techniques to address the issue of serious imbalances in the class distribution in real data if necessary.
Analysis of influencing factors of pneumatic flow enhancement of pumped concrete based on discrete element method
Authors: Su Chang , Wu Zhuang , Xu Hua , Wang Liu
Published: September 2022

It is known that, long-distance concrete pipeline transportation often causes pipe blocking accidents. For the problem of pipeline blockage, we have invented a swirl speed-increasing device. In this study, the discrete element method was used to calibrate the parameters of the contact model through the slump bucket experiment, and a discrete element model of horizontal pumping concrete was established. Based on the particle unit, the flow-promoting mechanism of the swirl speed-increasing device was studied, and the effects of the pumping speed, the volume fraction of coarse aggregate and the wind speed output of the device on the movement state of the particles and the flow-promoting distance were analyzed. The results show that the maximum velocity and flow-promoting distance of the particle unit increase with the increase of pumping speed and wind speed, and decrease with the increase of the coarse aggregate volume fraction. When the pumping speed and wind speed are large, the installation distance of the flow promoting device should be increased. When the volume fraction of concrete aggregate is large, the installation distance of the flow promoting device should be shortened. Our swirl speed-increasing device can solve the problem of pumping concrete pipeline blockage. The discrete element model of pumping concrete established in this study can well reflect the motion state of the concrete unit in the pipeline, which verifies the applicability of the discrete element model. The numerical simulation conclusion in this paper can avoid system energy waste and pipeline blockage caused by improper selection of device installation spacing.
Effect of weight loss on pregnancy outcomes, neuronal-reproductive-metabolic hormones and gene expression profiles in granulosa cells in obese infertile PCOS patients undergoing IVF-ET
Authors: Wu Limin , Fang Qunying , Wang Mengli , Wang Yurui , Zhu Xinyi , Fang Zhaohui , Lu Fangting , Xu Bo , Jin Rentao , Han Hui , Tong Xianhong
Published: September 2022

Objective

To investigate the effect of weight loss on pregnancy outcomes, PCOS related neuronal-reproductive-metabolic hormones and ovarian granulosa cell gene expression profiles in obese PCOS infertile patients undergoing in vitro fertilization-embryo transfer (IVF-ET).

Methods

75 patients undergoing IVF-ET due to tubal factors alone collected as the control group (group A), and 352 patients with obese PCOS infertility were divided into four groups according to the amount of weight loss before IVF: 0 kg (group B), 1-5 kg (group C), 5-10 kg (group D), and >10 kg (group E). Six cases of ovarian granulosa cells were collected randomly with the random number table method in each group for detecting mRNA profiling. Pathway networks and biological functions of the differentially expressed genes were analyzed. Validation by RT-PCR was performed.

Results

(1) The levels of luteinizing hormone(LH), testosterone(T) and homeostasis model assessment insulin resistance(HOMA-IR) in group E were significantly lower than those in groups B and C (P<0.05). (2) Compared with groups A and E, groups B and C showed increased total gonadotropin (Gn) and days of Gn stimulation (P<0.05), and the E2 level on trigger day and number of oocytes obtained in group B was significantly less than that in group E (P<0.05 or 0.01). Embryo implantation rate, clinical pregnancy rate and live birth rate were increased and miscarriage rate was decreased in groups A, D and E compared with group B (P<0.05 or 0.01). (3) There were significant differences among the control group and PCOS groups in some genes that are involved in neuronal-reproductive-metabolic endocrine, transcriptional regulation, cell proliferation and differentiation, etc (P<0.05). RNA-Seq results were validated by real time PCR analysis for the expression of follicle stimulating hormone receptor (FSHR), drosophila mothers against decapentaplegic protein 7(Smad7) and glutathione peroxidase 3(GPX3) genes that are known to have an important role in follicular development. Functional alterations were confirmed by the improvement in the ovarian responsiveness to Gn and embryo quality.

Conclusion

Weight loss more than 5kg may regulate the neuroreproductive endocrine hormone secretion, insulin resistance and gene expression profiles of ovarian granulosa cells, so as to improve the ovarian responsiveness to Gn, the embryo quality, embryo implantation rate, clinical pregnancy rate, live birth rate, and reduce the spontaneous abortion rate in obese infertile PCOS patients undergoing IVF-ET.

Clinical trial registration

www.chictr.org.cn, identifier ChiCTR1800018298.
Renal fat fraction is significantly associated with the risk of chronic kidney disease in patients with type 2 diabetes
Authors: Shen Yan , Xie Lianghua , Chen Xiangjun , Mao Lina , Qin Yao , Lan Rui , Yang Shumin , Hu Jinbo , Li Xue , Ye Hanwen , Luo Wenjin , Gong Lilin , Li Qifu , Mao Yun , Wang Zhihong
Published: September 2022

Backgrounds

Ectopic fat deposition is closely related to chronic kidney disease (CKD). Currently, there are few population studies that have been conducted to determine the relationship between renal parenchyma fat deposition and the risk of CKD among patients with type 2 diabetes mellitus (T2DM). Therefore, we employed magnetic resonance imaging (MRI) to detect renal parenchyma fat content in individuals with T2DM, expressed as renal fat fraction (FF), to explore whether renal FF is an important risk factor for CKD in patients with T2DM.

Methods

In this cross-sectional study, 189 subjects with T2DM were enrolled. CKD was defined as the estimated glomerular filtration rate (eGFR)<60 mL/min/1.73m2. Measurement of the renal FF was performed on a 3.0-T MRI (MAGNETOM Skyra, Siemens, Erlangen, Germany). Binary logistic regression was used to determine the association between tertiles of renal FF and risk of CKD. Receiver-operator characteristic (ROC) curves were constructed to evaluate the sensitivity and specificity of renal FF in detecting CKD in T2DM patients.

Results

The patients were divided into three groups according to tertiles of the renal FF level (2.498 - 7.434). As renal FF increases, patients tend to be older, and more abdominally obese, with a decreased eGFR (p<0.05). After adjustment for potential confounders, patients in the highest tertile of renal FF had a significantly increased risk of CKD than those in the lowest tertile (odds ratio (OR) = 3.98, 95% confidence interval (CI) = 1.12 - 14.09, p = 0.032), and the area under the ROC curve for this model was 0.836 (0.765–0.907).

Conclusions

The renal FF is significantly independently associated with CKD in patients with T2DM.
Impact of relocation and reconstruction policies on the upgrading of urban industrial structure in old industrial districts
Authors: Lin Qixing , Luo Xiang , Lin Guodong , Yang Tiantian , Su Wenjie
Published: September 2022

Old industrial bases (municipal districts) play a decisive role in the process of China’s economic development. This study focuses on the upgrading of urban industrial structure. Based on the panel data of 120 old industrial bases (municipal districts) in China from 2010 to 2018, a double difference model was established to empirically analyze the impact of pilot relocation and reconstruction policies on the upgrading of urban industrial structure in old industrial districts. The results demonstrate that 1) The pilot relocation and reconstruction policies play a significant role in promoting the upgrading of urban industrial structure in old industrial districts; 2) Upgrading of urban industrial structure is significantly affected by the urban economic development level, urban population density, land marketization level, industrial enterprise agglomeration degree and infrastructure construction; 3) The impact of relocation and reconstruction policies on the upgrading of urban industrial structure in old industrial districts exhibits certain regional heterogeneity. Finally, some targeted suggestions are proposed for future formulation of effective policies for the relocation and reconstruction of old industrial areas and optimal allocation of urban industrial structure.
The production function socialization trend of rural housing land and its response to rural land planning in metropolitan suburbs from the perspective of rural space commodification
Authors: Zhao Qinglei , Jiang Guanghui , Ma Wenqiu , Yang Yuting , Zhou Tao
Published: September 2022

An investigation of the changing production function of rural housing land can help to guide appropriate land use adjustment and rural land planning. Taking into account the layout characteristics from 2005 to 2018, we employed the structural equation model and the theory of planned behavior to analyze the differentiation mechanism of rural housing land production function based on survey data of 613 typical farmers in Pinggu District of Beijing. Our results show that, first, the production function intensity of rural housing land in Pinggu District fell from 0.327 to 0.126, and the coefficient of variation increased from 0.15 to 0.54. This indicates that the overall production function decreased but the spatial heterogeneity increased. Second, the production function of rural housing land gradually withdrew and socialized in villages, following the socialization evolution process of agricultural production function, industrial and commercial service function, and public service function. Third, the internal land use pattern of rural housing land is significantly influenced by the subjective norms and perceptual behavior control of farmers. The demonstrative norms of family and the prescriptive norms of village collective have significant effects on subjective norms, and the perceived behavioral control is significantly affected by income scale and structure, family size, and employment type. Finally, to further promote rural housing land management, it is necessary to optimize its prescriptive norms of the village collective, improve the farmers’ income level and employment structure, and weaken the farmers’ perceived difficulties. According to the different function socialization stages of rural housing land, rural land planning should coordinate the relationship between the production function socialization of rural housing land and the commercialization of rural space, and boost the revitalization of rural industry.
Development of a clinically validated in vitro functional assay to assess pathogenicity of novel GAA variants in patients with Pompe disease identified via newborn screening
Authors: Goomber Shelly , Huggins Erin , Rehder Catherine W. , Cohen Jennifer L. , Bali Deeksha S. , Kishnani Priya S.
Published: September 2022

Purpose: The addition of Pompe disease (Glycogen Storage Disease Type II) to the Recommended Uniform Screening Panel in the United States has led to an increase in the number of variants of uncertain significance (VUS) and novel variants identified in the GAA gene. This presents a diagnostic challenge, especially in the setting of late-onset Pompe disease when symptoms are rarely apparent at birth. There is an unmet need for validated functional studies to aid in classification of GAA variants.

Methods: We developed an in vitro mammalian cell expression and functional analysis system based on guidelines established by the Clinical Genome Resource (ClinGen) Sequence Variant Interpretation Working Group for PS3/BS3. We validated the assay with 12 control variants and subsequently analyzed eight VUS or novel variants in GAA identified in patients with a positive newborn screen for Pompe disease without phenotypic evidence of infantile-onset disease.

Results: The control variants were analyzed in our expression system and an activity range was established. The pathogenic controls had GAA activity between 0% and 11% of normal. The benign or likely benign controls had an activity range of 54%–100%. The pseudodeficiency variant had activity of 17%. These ranges were then applied to the variants selected for functional studies. Using the threshold of <11%, we were able to apply PS3_ supporting to classify two variants as likely pathogenic (c.316C > T and c.1103G > A) and provide further evidence to support the classification of likely pathogenic for two variants (c.1721T > C and c.1048G > A). One variant (c.1123C > T) was able to be reclassified based on other supporting evidence. We were unable to reclassify three variants (c.664G > A, c.2450A > G, and c.1378G > A) due to insufficient or conflicting evidence.

Conclusion: We investigated eight GAA variants as proof of concept using our validated and reproducible in vitro expression and functional analysis system. While additional work is needed to further refine our system with additional controls and different variant types in order to apply the PS3/BS3 criteria at a higher level, this tool can be utilized for variant classification to meet the growing need for novel GAA variant classification in the era of newborn screening for Pompe disease.
Genome-wide analysis of OFP gene family in pepper (Capsicum annuum L.)
Authors: Luo Yin , Yang Shimei , Luo Xirong , Li Jing , Li Tangyan , Tang Xiangqun , Liu Feng , Zou Xuexiao , Qin Cheng
Published: September 2022

Ovate family proteins (OFPs) are transcriptional inhibitors that regulate plant growth and development and play important roles in the synthesis of secondary cell walls during pollen development. This study identified the pepper OFP gene family based on the genome-wide analysis and used bioinformatics methods to provide a fundamental profile of the gene family. 74 OFP genes with typical Ovate domain were identified in cultivated pepper Zunla-1, wild pepper Chiltepin and CM334. Chromosome mapping revealed that CazOFP genes were unevenly distributed on 11 chromosomes and Chr00 in Zunla-1, CacOFP genes on 12 chromosomes in Chiltepin, and CamOFP genes on 12 chromosomes and two Scaffflods in CM334. Gene structure analysis revealed that CaOFP genes possessed 1-3 exons, and the analysis of physicochemical properties suggested that CaOFPs were hydrophilic. Many cis-acting elements were identified in the promoter region of CaOFP genes, including ABRE, ARE, Box 4, G-box, TC-rich, and TCT-motif. The expression patterns of pepper at different growth stages showed that CaOFP genes were actively involved in the growth and fruit development of pepper, and CazOFP16 and CazOFP17 were actively involved in response to multiple hormones and stress events. qRT-PCR was also used to verify the expression of CazOFP gene in two developmental stages of seven pepper varieties with different fruit shapes, and it was found that CaOFP genes may be involved in the formation of fruit type in pepper. This study provides theoretical and practical evidence for future research on the OFP gene family.
Identification of N6-methylandenosine related lncRNA signatures for predicting the prognosis and therapy response in colorectal cancer patients
Authors: Li Zhiyong , Liu Yang , Yi Huijie , Cai Ting , Wei Yunwei
Published: September 2022

Despite recent advances in surgical and multimodal therapies, the overall survival (OS) of advanced colorectal cancer (CRC) patients remains low. Thus, discerning sensitive prognostic biomarkers to give the optimistic treatment for CRC patients is extremely critical. N6-methyladenosine (m6A) and long noncoding RNAs (lncRNAs) play an important role in CRC progression. Nonetheless, few studies have focused on the impact of m6A-related lncRNAs on the prognosis, tumor microenvironment (TME) and treatment of CRC. In this study, 1707 m6A-related lncRNAs were identified through Pearson correlation analysis and Weighted co-expression network analysis (WGCNA) using The Cancer Genome Atlas (TCGA) cohort. Then, 28 m6A-related prognostic lncRNAs were screened by univariate Cox regression analysis, followed by identifying two clusters by consensus clustering analysis. A prognostic model consisted of 8 lncRNA signatures was constructed by the least absolute shrinkage and selection operator (LASSO). Kaplan–Meier curve analysis and a nomogram were performed to investigate the prognostic ability of this model. The risk score of prognostic model act as an independent risk factor for OS rate. Functional enrichment analysis indicated that lncRNA signatures related tumor immunity. The low-risk group characterized by increased microsatellite instability-high (MSI-H), mutation burden, and immunity activation, indicated favorable odds of OS. Moreover, the lncRNA signatures were significantly associated with the cancer stem cell (CSC) index and drug sensitivity. In addition, 3 common immune genes shared by the lncRNA signatures were screened out. We found that these immune genes were widely distributed in 2 cell types of TME. Finally, a ceRNA network was constructed to identify ZEB1-AS1 regulatory axis in CRC. We found that ZEB1-AS1 was significantly overexpressed in tumor tissues, and was related to the metastasis of EMT and the chemoresistance of 5-Fu in CRC. Therefore, our study demonstrated the important role of m6A-related lncRNAs in TME remodeling. Moreover, these results illustrated the levels of ZEB1-AS1 might be valuable for predicting the progression and prognosis of CRC, and further provided a new target for the diagnosis and treatment of CRC patients.