bluuebunny/crossref_metadata_2025 huggingface dataset:
Flavonoid Nobiletin Attenuates Cyclophosphamide-Induced Cystitis in Mice through Mechanisms That Involve Inhibition of IL-1β Induced Connexin 43 Upregulation and Gap Junction Communication in Urothelial Cells
Authors: Kono Jin , Ueda Masakatsu , Sengiku Atsushi , Suadicani Sylvia O. , Woo Je Tae , Kobayashi Takashi , Ogawa Osamu , Negoro Hiromitsu
Published: May 2022

Bladder inflammatory diseases cause various urinary symptoms, such as urinary frequency and painful urination, that impair quality of life. In this study, we used a mouse model of cyclophosphamide (CYP)-induced bladder inflammation and immortalized human urothelial (TRT-HU1) cells to explore the preventive potential of nobiletin (NOB), a polymethoxylated flavone enriched in citrus fruit peel, and investigate its mechanism of action in the bladder. Prophylaxis with PMF90 (60% NOB) attenuated the development of bladder inflammation and urinary symptoms in CYP-treated mice. PMF90 also reduced the upregulation of connexin 43 (Cx43), a major component of gap junction channels, in the bladder mucosa of CYP-treated mice. Stimulation of TRT-HU1 cells with the pro-inflammatory cytokine IL-1β increased Cx43 mRNA and protein expression and enhanced gap junction coupling—responses that were prevented by pre-treatment with NOB. In urothelium-specific Cx43 knockout (uCx43KO) mice, macroscopic signs of bladder inflammation and changes in voiding behavior induced by CYP treatment were significantly attenuated when compared to controls. These findings indicate the participation of urothelial Cx43 in the development of bladder inflammation and urinary symptoms in CYP-treated mice and provide pre-clinical evidence for the preventive potential of NOB through its anti-inflammatory effects on IL-1β signaling and urothelial Cx43 expression.
Association of Ligamentum Flavum Hypertrophy with Adolescent Idiopathic Scoliosis Progression—Comparative Microarray Gene Expression Analysis
Authors: Seki Shoji , Iwasaki Mami , Makino Hiroto , Yahara Yasuhito , Kondo Miho , Kamei Katsuhiko , Futakawa Hayato , Nogami Makiko , Watanabe Kenta , Tran Canh Tung Nguyen , Hirokawa Tatsuro , Tsuji Mamiko , Kawaguchi Yoshiharu
Published: May 2022

The role of the ligamentum flavum (LF) in the pathogenesis of adolescent idiopathic scoliosis (AIS) is not well understood. Using magnetic resonance imaging (MRI), we investigated the degrees of LF hypertrophy in 18 patients without scoliosis and on the convex and concave sides of the apex of the curvature in 22 patients with AIS. Next, gene expression was compared among neutral vertebral LF and LF on the convex and concave sides of the apex of the curvature in patients with AIS. Histological and microarray analyses of the LF were compared among neutral vertebrae (control) and the LF on the apex of the curvatures. The mean area of LF in the without scoliosis, apical concave, and convex with scoliosis groups was 10.5, 13.5, and 20.3 mm2, respectively. There were significant differences among the three groups (p < 0.05). Histological analysis showed that the ratio of fibers (Collagen/Elastic) was significantly increased on the convex side compared to the concave side (p < 0.05). Microarray analysis showed that ERC2 and MAFB showed significantly increased gene expression on the convex side compared with those of the concave side and the neutral vertebral LF cells. These genes were significantly associated with increased expression of collagen by LF cells (p < 0.05). LF hypertrophy was identified in scoliosis patients, and the convex side was significantly more hypertrophic than that of the concave side. ERC2 and MAFB genes were associated with LF hypertrophy in patients with AIS. These phenomena are likely to be associated with the progression of scoliosis.
A Real-Time Fatigue Sensing and Enhanced Feedback System
Authors: Miao Xinyue , Xue Chengqi , Li Xian , Yang Lichun
Published: May 2022

This paper describes a real-time fatigue sensing and enhanced feedback system designed for video terminal operating groups. This paper analyzes the advantages and disadvantages of various current acquisition devices and various algorithms for fatigue perception. After comparison, this study uses an eye movement instrument to collect user PERCLOS, and then calculates and determines the user’s fatigue state. A detailed fatigue discrimination calculation method is provided in this paper. The fatigue level is divided into three levels: mild fatigue, moderate fatigue and severe fatigue. Finally, this study uses the fatigue method demonstrated above to achieve real-time discrimination of the fatigue level of the user in front of the video operation terminal. This paper elaborates a method for waking up users and enhancing feedback based on their fatigue level and the importance of information. This study provides a solution for avoiding the operational risks caused by fatigue and lays the foundation for the machine to sense the user and provide different service solutions based on the user’s status.
ICT Governance and Management Macroprocesses of a Brazilian Federal Government Agency
Authors: Canedo Edna Dias , do Vale Ana Paula Morais , Gravina Rogério Machado , de Vasconcelos Sales Alessandra , Praciano Bruno J. G. , dos Reis Vinicius Eloy , Mendonça Fábio Lúcio Lopes , de Sousa Júnior Rafael Timóteo
Published: May 2022

The process of identifying and managing Information and Communication Technology (ICT) risks has become a concern and a challenge for public and private organizations. In this context, risk management methodologies within the Brazilian Federal Public Administration organizations have become indispensable to help the managers of these organizations in decision making, especially in the distribution of public funds, elaboration of public policies focused on transparency, social actions contemplating indemnities, and social benefits, among others. In addition, the various ICT projects controlled by the public administration need a methodology to perform their management of ICT resources. In this article, we present the Governance and Risk Management methodology used to model the Administrative Council for Economic Defense (CADE) macro processes. The proposed methodology used the risk management process aligned to the ISO 31000 standards. This alignment was necessary for mapping CADE’s risk events, regardless of their complexity. The modeled ICT risk processes will support the organization’s managers in decision making and may be used or customized by any other organization of the Brazilian Federal Public Administration.
An Exploratory Study on the Validation of THUNDERS: A Process to Achieve Shared Understanding in Problem-Solving Activities
Authors: Agredo-Delgado Vanessa , Ruiz Pablo H. , Collazos Cesar A. , Moreira Fernando
Published: May 2022

The complexity in collaborative work is mainly related to the difficulty in social interaction, which generates low levels of understanding among participants about what they should do and about the problem to be solved, resulting in problems in the motivation to generate true collaboration. Therefore, in the search to improve collaborative work and encourage this collaboration, it is necessary to implement strategies that promote the construction of shared understanding and obtain better group results. However, building it becomes a challenge due to the factors that influence it and how little is known about its construction. In this sense, to improve collaborative work, as a result of a research process, the THUNDERS process is proposed, which provides a set of elements to build shared understanding in problem-solving activities and with heterogeneous group formation. Specifically, this paper presents the results of the statistical validation of THUNDERS through the Student’s t-test, which was used in an exploratory study in the educational field in two Colombian universities, where learning styles were considered for the formation of groups; having groups that used the process and other control groups that did not use it, the collaborative activity consisted of determining the scope of a process line simulating a software development company. According to the results obtained in the context of this study, it can be considered that THUNDERS encourages and improves shared understanding when people in a group work collaboratively to solve a problem. In addition, elements for improvement were identified that should be incorporated in further stages of this research so that the process allows for an easy and guided construction of shared understanding in any application context.
Genotype-by-Diet Interactions for Larval Performance and Body Composition Traits in the Black Soldier Fly, Hermetia illucens
Authors: Sandrock Christoph , Leupi Simon , Wohlfahrt Jens , Kaya Cengiz , Heuel Maike , Terranova Melissa , Blanckenhorn Wolf U. , Windisch Wilhelm , Kreuzer Michael , Leiber Florian
Published: May 2022

Further advancing black soldier fly (BSF) farming for waste valorisation and more sustainable global protein supplies critically depends on targeted exploitation of genotype-phenotype associations in this insect, comparable to conventional livestock. This study used a fully crossed factorial design of rearing larvae of four genetically distinct BSF strains (FST: 0.11–0.35) on three nutritionally different diets (poultry feed, food waste, poultry manure) to investigate genotype-by-environment interactions. Phenotypic responses included larval growth dynamics over time, weight at harvest, mortality, biomass production with respective contents of ash, fat, and protein, including amino acid profiles, as well as bioconversion and nitrogen efficiency, reduction of dry matter and relevant fibre fractions, and dry matter loss (emissions). Virtually all larval performance and body composition traits were substantially influenced by diet but also characterised by ample BSF genetic variation and, most importantly, by pronounced interaction effects between the two. Across evaluated phenotypes, variable diet-dependent rankings and the lack of generally superior BSF strains indicate the involvement of trade-offs between traits, as their relationships may even change signs. Conflicting resource allocation in light of overall BSF fitness suggests anticipated breeding programs will require complex and differential selection strategies to account for pinpointed trait maximisation versus multi-purpose resilience.
Temperature Dependence for Survival, Development, and Reproduction of the Cactus Cochineal Dactylopius opuntiae (Cockerell)
Authors: El Aalaoui Mohamed , Sbaghi Mohamed
Published: May 2022

The effect of temperature on Dactylopius opuntiae (Cockerell) life cycle parameters was evaluated at 20, 23, 26, 32, and 40 ± 1 °C, 65 ± 5% RH, and a photoperiod of 12 L:8 D. Temperatures ranging from 26 °C to 32 °C were suitable for survival, development, and reproduction of D. opuntiae. The total developmental time of females ranged from 94.23 d (20 °C) to 43.55 d (40 °C). The average development time of males from egg to death ranged from 26.97 days at 32 °C to 50.75 days at 20 °C. The probability that a newly laid egg would survive to the adult stage was highest at 26 °C and 32 °C (44–60%). The parthenogenesis in females was not observed during our study. The longest oviposition period was observed when the cochineal was reared at 32 °C (17.97 days), and the highest fecundity was observed at 32 °C (355.29 egg/female). The highest proportion of females (0.80) was observed at 40 °C. According to the age-stage-two-sex life table, the highest value of the intrinsic rate of natural increase (rm) was recorded at 32 °C. The lower developmental thresholds for the total pre-adult female and male and adult female and male stages, were 10.15, 12.21, 10.54, and 21.04 °C, respectively. Dactylopius opuntiae females needed a higher thermal constant (769.23 D°) than males (357.14 D°) to achieve their development and reach the mature adult stage. These findings will be useful for the development of an integrated pest management strategy for D. opuntiae.
Association of Catheter Ablation and Reduced Incidence of Dementia among Patients with Atrial Fibrillation during Long-Term Follow-Up: A Systematic Review and Meta-Analysis of Observational Studies
Authors: Saglietto Andrea , Ballatore Andrea , Xhakupi Henri , De Ferrari Gaetano Maria , Anselmino Matteo
Published: May 2022

Background: Atrial fibrillation (AF) is independently associated with the onset of cognitive decline/dementia. AF catheter ablation (AFCA) is the most effective treatment strategy in terms of sinus rhythm maintenance, but its effects on dementia prevention remain under investigation. The aim of the present study was to perform a systematic review and meta-analysis of the presently available studies exploring the effect of AFCA on dementia occurrence. Methods: PubMed/MEDLINE databases were screened for articles through 14 March 2022 reporting adjusted time-to-event outcome data comparing AFCA and non-AFCA cohorts in terms of de novo dementia occurrence. A random effect meta-analysis was performed to estimate the meta-analytic hazard ratio (HR) of dementia occurrence in AFCA vs. non-AFCA cohorts, as well as the meta-analytic incidence rate of dementia in the non-AFCA cohort. Based on the aforementioned estimates, the number needed to treat (NNT), projected at median follow-up, was derived. Results: Four observational studies were included in the analysis, encompassing 40,146 patients (11,312 in the AFCA cohort; 28,834 in the non-AFCA cohort). AFCA conferred a significant protection to the development of dementia with an overall HR of 0.52 (95% CI 0.35–0.76). The incidence rate of dementia in the non-AFCA group was 1.12 events per 100 person-year (95% CI 0.47–2.67). The derived NNT projected to the median follow-up (4.5 years) was 41. Conclusion: AFCA is associated with a nearly 50% reduction in dementia occurrence during a median 4.5-year follow-up. Future randomized clinical trials are needed to reinforce these findings.
Clonal Tracing of Heart Regeneration
Authors: Kolluri Kamal , Nazarian Taline , Ardehali Reza
Published: May 2022

Cardiomyocytes in the adult mammalian heart have a low turnover during homeostasis. After myocardial injury, there is irreversible loss of cardiomyocytes, which results in subsequent scar formation and cardiac remodeling. In order to better understand and characterize the proliferative capacity of cardiomyocytes, in vivo methods have been developed to track their fate during normal development and after injury. Lineage tracing models are of particular interest due to their ability to record cell proliferation events over a long period of time, either during development or in response to a pathological event. This paper reviews two well-studied lineage-tracing, transgenic mouse models—mosaic analysis with double markers and rainbow reporter system.
A Comprehensive Update on Retinal Vasculitis: Etiologies, Manifestations and Treatments
Authors: Agarwal Aniruddha , Rübsam Anne , zur Bonsen Lynn , Pichi Francesco , Neri Piergiorgio , Pleyer Uwe
Published: May 2022

Retinal vasculitis is characterized by inflammatory involvement of retinal arterioles, venules and/or capillaries and can be associated with a myriad of systemic and ophthalmic diseases. In this review, we have comprehensively discussed the etiologies, clinical manifestations, and presentations of retinal vasculitis. We have also included newer advances in imaging in retinal vasculitis such as OCTA and widefield imaging.
IL-6 Responsiveness of CD4+ and CD8+ T Cells after Allogeneic Stem Cell Transplantation Differs between Patients and Is Associated with Previous Acute Graft versus Host Disease and Pretransplant Antithymocyte Globulin Therapy
Authors: Tvedt Tor Henrik Anderson , Rose-John Stefan , Tsykunova Galina , Ahmed Aymen Bushra , Gedde-Dahl Tobias , Ersvær Elisabeth , Bruserud Øystein
Published: May 2022

Graft-versus-host disease (GVHD), one of the most common and serious complications after allogeneic stem cell transplantation, is mediated by allocative T cells. IL-6 mediates both pro- and anti-inflammatory effects and modulates T cell response through classical signaling and trans-signaling. We investigated the effects on the mTOR and JAK/STAT pathways after various types of IL-6 signaling for circulating T cells were derived from 31 allotransplant recipients 90 days post-transplant. Cells were stimulated with IL-6 alone, hyper-IL-6 (trans-signaling), IL-6+IL-6 receptor (IL-6R; classical + trans-signaling) and IL-6+IL-6R+soluble gp130-Fc (classical signaling), and flow cytometry was used to investigate the effects on phosphorylation of AKT (Thr308), mTOR (Ser2442), STAT3 (Ser727) and STAT3 (Tyr705). CD3+CD4+ and CD3+C8+ T cells responded to classical and trans IL-6 stimulation with increased STAT3 (Tyr705) phosphorylation; these responses were generally stronger for CD3+CD4+ cells. STAT3 (Tyr705) responses were stronger for patients with previous acute GVHD; CD3+CD4+ cells from GVHD patients showed an additional STAT3 (Ser727) response, whereas patients without acute GVHD showed additional mTOR (Ser2448) responses. Furthermore, treatment with antithymocyte globulin as a part of GVHD prophylaxis was associated with generally weaker STAT3 (Tyr705) responses and altered STAT3 (Ser727) responsiveness of CD3+CD4+ cells together with increased mTOR (Ser2448) responses for the CD3+CD8+ cells. Thus, early post-transplant CD3+CD4+ and CD3+ CD8+ T cell subsets differ in their IL-6 responsiveness; this responsiveness is modulated by antithymocyte globulin and differs between patients with and without previous acute GVHD. These observations suggest that allotransplant recipients will be heterogeneous with regard to the effects of post-transplant IL-6 targeting.
Risk Stratification in Patients with Acute Pulmonary Embolism: Current Evidence and Perspectives
Authors: Leidi Antonio , Bex Stijn , Righini Marc , Berner Amandine , Grosgurin Olivier , Marti Christophe
Published: May 2022

Risk stratification is one of the cornerstones of the management of acute pulmonary embolism (PE) and determines the choice of both diagnostic and therapeutic strategies. The first step is the identification of patent circulatory failure, as it is associated with a high risk of immediate mortality and requires a rapid diagnosis and prompt reperfusion. The second step is the estimation of 30-day mortality based on clinical parameters (e.g., original and simplified version of the pulmonary embolism severity index): low-risk patients without right ventricular dysfunction are safely managed with ambulatory anticoagulation. The remaining group of hemodynamically stable patients, labeled intermediate-risk PE, requires hospital admission, even if most of them will heal without complications. In recent decades, efforts have been made to identify a subgroup of patients at an increased risk of adverse outcomes (intermediate-high-risk PE), who might benefit from a more aggressive approach, including reperfusion therapies and admission to a monitored unit. The cur-rent approach, combining markers of right ventricular dysfunction and myocardial injury, has an insufficient positive predictive value to guide primary thrombolysis. Sensitive markers of circulatory failure, such as plasma lactate, have shown interesting prognostic accuracy and may play a central role in the future. Furthermore, the improved security of reduced-dose thrombolysis may enlarge the indication of this treatment to selected intermediate–high-risk PE.
Ultrasound-Guided Tru-Cut Biopsy in Gynecological and Non-Gynecological Pelvic Masses: A Single-Center Experience
Authors: Buonomo Francesca , Bussolaro Sofia , de Almeida Fiorillo Clarice , Oliveira de Souza Danilo , Giudici Fabiola , Romano Federico , Romano Andrea , Ricci Giuseppe
Published: May 2022

Aim: The aim of this study was to evaluate the feasibility of adequacy, accuracy, and safety of ultrasound-guided tru-cut biopsy in managing malignant and benign abdominopelvic masses in a selected population and critically discuss some issues in different situations, which deserve some reflections on those practices. Materials and Methods: This is a retrospective study involving 42 patients who underwent transvaginal or transabdominal tru-cut biopsy between August 2017 and November 2021. The inclusion criteria were poor health status or primary inoperable advanced tumor, suspicion of recurrence or metastasis to the ovaries or peritoneum in gynecological and non-gynecological pelvic malignancies. Tissue samples were considered adequate if it was possible to determine the origin of the tumor, and immunohistochemistry could be performed. Diagnostic accuracy was assessed considering the agreement between tru-cut biopsy histology and final postoperative histology. Results: It total, 44 biopsies were obtained from 42 patients (2 patients had repeat biopsies). The pathologist considered all pathological samples adequate (adequacy 100%). The final histology was consistent with tru-cut biopsy diagnosis in all but 2 cases (diagnostic accuracy 88.2%). If we consider only the cases that have carried out at least two diagnostic samples, accuracy rose to 94.1%. Pathological examinations from tru-cut samples showed 2 benign lesions (4.8%) and 40 malignant tumors (95.2%), divided into 19 advanced primary inoperable ovarian cancers, 7 primary advanced cervical cancers, 4 recurrent endometrial cancers, 3 recurrent cervical cancers, 3 recurrent ovarian cancers, 1 case of primitive peritoneal malignancy (leiomyosarcoma), and 3 non-gynecological cancers with a strong suspicion of metastases at ultrasound (2 cases of ovarian, colorectal cancer metastasis, and 1 case of pelvic site type B lymphoma metastasis). However, one case of minor complication related to the procedure was reported but not significant. Conclusions: The diagnostic adequacy, accuracy of the tru-cut biopsy, and safety were high. Pathological samples are representative of the disease and suitable for histological and immunohistochemical analysis.
An Osteotomy Tool That Preserves Bone Viability: Evaluation in Preclinical and Clinical Settings
Authors: Bahat Oded , Yin Xing , Holst Stefan , Zabalegui Ion , Berroeta Eva , Pérez Javier , Wöhrle Peter , Sörgel Norbert , Brunski John , Helms Jill A.
Published: May 2022

The main objectives of this work were to assess the efficiency, ease-of-use, and general performance of a novel osseoshaping tool based on first-user clinical experiences and to compare these observations with preclinical data generated in rodents using a miniaturized version of the instrument. All patients selected for the surgery presented challenging clinical conditions in terms of the quality and/or quantity of the available bone. The presented data were collected during the implant placement of 15 implants in 7 patients, and included implant recipient site (bone quality and quantity) and ridge evaluation, intra-operative handling of the novel instrument, and the evaluation of subsequent implant insertion. The instrument was easy to handle and was applied without any complications during the surgical procedure. Its use obviated the need for multiple drills and enabled adequate insertion torque in all cases. This biologically driven innovation in implant site preparation shows improvements in preserving vital anatomical and cellular structures as well as simplifying the surgical protocol with excellent ease-of-use and handling properties.
Obstetric and Neonatal Outcomes following COVID-19 Vaccination in Pregnancy
Authors: Peretz-Machluf Ravit , Hirsh-Yechezkel Galit , Zaslavsky-Paltiel Inna , Farhi Adel , Avisar Nir , Lerner-Geva Liat , Meyer Raanan , Tsur Abraham , Yinon Yoav
Published: May 2022

COVID-19 infection imposes a risk for pregnant individuals and may lead to adverse maternal and obstetric outcomes. This is a retrospective cohort study of all women giving birth between March and July 2021 at a single tertiary center. Obstetric and neonatal outcomes were compared between vaccinated and non-vaccinated pregnant women with singleton pregnancies. Women with prior COVID-19 infection, multiple gestations and stillbirth were excluded from the study. Of 4708 women who delivered during the study period, 3700 met the eligibility criteria, of whom 3240 were vaccinated during pregnancy. Compared with the non-vaccinated group, the vaccinated group was characterized by a lower rate of smoking (3.70% vs. 6.67%, p = 0.0028), whereasother maternal characteristics were not significantly different. Multivariable analysis demonstrated that COVID-19 mRNA vaccination was not significantly associated with increased risk of preterm birth as well as other adverse obstetric outcomes including hypertensive diseases of pregnancy, cesarean delivery and small for gestational age. However, a significantly lower risk for meconium-stained amniotic fluid was observed among the vaccinated group (adjusted odds ratio 0.63; 95% confidence interval, 0.46–0.86, p = 0.0039). Moreover, the vaccine was not significantly associated with increased risk of neonatal adverse outcomes including respiratory complications and NICU hospitalization. In conclusion, BNT162b2 messenger RNA vaccination during pregnancy was not associated with an increased rate of adverse obstetric and neonatal outcomes. Therefore, in view of its safety on one hand, and the risk associated with COVID-19 disease in pregnancy on the other hand, BNT 162b2 COVID-19 vaccine should be recommended for pregnant women.
Bereaved Family Members’ Perspectives of Good Death and Quality of End-of-Life Care for Malignant Pleural Mesothelioma Patients: A Cross-Sectional Study
Authors: Nagamatsu Yasuko , Sakyo Yumi , Barroga Edward , Koni Riwa , Natori Yuji , Miyashita Mitsunori
Published: May 2022

Objective: This study investigated whether malignant pleural mesothelioma (MPM) patients achieved good deaths and good quality of end-of-life care compared with other cancer patients from the perspective of bereaved family members in Japan. Methods: This cross-sectional study was part of a larger study on the achievement of good deaths of MPM patients and the bereavement of their family members. Bereaved family members of MPM patients in Japan (n = 72) were surveyed. The Good Death Inventory (GDI) was used to assess the achievement of good death. The short version of the Care Evaluation Scale (CES) version 2 was used to assess the quality of end-of-life care. The GDI and CES scores of MPM patients were compared with those of a Japanese cancer population from a previous study. Results: MPM patients failed to achieve good deaths. Only 12.5% of the MPM patients were free from physical pain. The GDI scores of most of the MPM patients were significantly lower than those of the Japanese cancer population. The CES scores indicated a significantly poorer quality of end-of-life care for the MPM patients than the Japanese cancer population. The total GDI and CES scores were correlated (r = 0.55). Conclusions: The quality of end-of-life care for MPM patients remains poor. Moreover, MPM patients do not achieve good deaths from the perspective of their bereaved family members.
Effect of PCSK9 Inhibitors on Hemostasis in Patients with Isolated Hypercholesterolemia
Authors: Basiak Marcin , Hachula Marcin , Kosowski Michal , Okopien Boguslaw
Published: May 2022

Background: In addition to reducing plasma lipids, proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors may produce numerous nonlipid-related pleiotropic effects. The purpose of this trial was to determine the efficacy of PCSK9 inhibitors alone in patients with isolated hypercholesterolemia. Methods: The trial enrolled 21 individuals with isolated hypercholesterolemia and atherosclerosis who received alirocumab for 90 days (150 mg every two weeks). Lipids, glucose homeostasis factors, and hemostatic markers were measured in the plasma at baseline and after treatment. Results: The PCSK9 inhibitor administered to these patients reduced plasma levels/activity of fibrinogen (from 3.6 ± 0.5 to 2.9 ± 0.4 g/L, p < 0.01), factor VII (from 143.8 ± 16.7 to 114.5 ± 14.1%, p < 0.01) and plasminogen activator inhibitor-1 (PAI-1) (from 74.9 ± 13.9 to 52.8 ± 9.1 ng/mL, p < 0.001) without a significant reduction in von Willebrand factor levels, and it tended to prolong the partial thromboplastin and prothrombin times. Conclusion: Our findings indicate that treatment with PCSK9 inhibitors has a multipotential effect on fibrinolysis and coagulation in patients with isolated hypercholesterolemia and that this medication may have some future benefits in patients who are statin-intolerant or contraindicated to statin use.
Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam
Authors: Kien Truong Quy , Toan Pham Quoc , Nghia Phan Ba , Van Diem Thi , Duc Nguyen Van , Ha Do Manh , Dung Nguyen Thi Thuy , Ha Nguyen Thi Thu , Quyen Le Thi Bao , Vinh Hoang Trung , Manh Bui Van , Su Hoang Xuan , Tien Tran Viet , Thang Le Viet , Rostaing Lionel
Published: May 2022

Objectives: The purpose of this study was to identify the SNP sites and determine the BKV genotype circulating in kidney-transplant Vietnamese recipients based on the VP1 gene region. Methods: 344 samples were collected from post-kidney-transplant recipients at the 103 Vietnam Military Hospital to investigate the number of BKV infections. Positive samples with a sufficient virus concentration were analyzed by nested PCR in the VP1 region, sequencing detected genotyping and single-nucleotide polymorphism. Results: BKV infection was determined in 214 patients (62.2%), of whom 11 (5.1%) were diagnosed with BKV-associated nephropathy. Among the 90 BKV-I strains sequenced, 89 (98.88%) were strains of I/b-1 and 1 (1.12%) was strain I/b-2. The 60 BKV-IV strains had a greater diversity of subgroups, including 40% IV/a-1, 1.66% IV/a-2, 56.68% IV/c-1, and 1.16% IV/c-2. Additionally, of 11 cases diagnosed with BKVN, seven belonged to subgroup I/b-1 (63.6%) and four to subgroup IV/c-1 (36.4%). Moreover, 22 specific SNPs that were genotype I or IV were determined in this Vietnamese population. Specifically, at position 1745, for the Vietnamese BKV-IV strains, the SNP position (A→G) appeared in 57/60 samples (95%). This causes transformation of the amino acid N→S. This SNP site can enable detection of genotype IV in Vietnam. It represents a unique evolution pattern and mutation that has not been found in other international strains. Conclusion: The BKV-I genotype was more common than BKV-IV; however, mutations that occur on the VP1 typing region of BKV-IV strains were more frequent than in BKV-I strains.
Transesophageal Echocardiography-Guided Transseptal Left Atrial Access to Improve Safety in Patients Undergoing Pulmonary Vein Isolation
Authors: Zuercher Rahel , Herling Anique , Schmidt Marc T. , Bachmann Marta , Winnik Stephan , Duru Firat , Eriksson Urs
Published: May 2022

Background: Endovascular pulmonary vein isolation (PVI) has become an important strategy for rhythm control in patients with symptomatic atrial fibrillation (AF). Transseptal access is a critical step of this procedure and can result in potentially life-threatening complications. This retrospective study evaluates the safety of standardized, transesophageal echocardiography (TEE)-guided transseptal access to the left atrium in consecutive patients who underwent PVI. Methods: After the implementation of a standardized, TEE-guided procedure for transseptal access, the data of 404 consecutive PVI procedures using radiofrequency ablation and 3D-mapping were prospectively collected over 5 years. TEE-guided transseptal punctures were performed on 375 patients undergoing one to three PVIs. The patient cohort was retrospectively analyzed for major and minor complications, fluoroscopy time, fluoroscopy dose and ablation outcomes. Results: No single complication related to transseptal access occurred, affirming the safety of the TEE-guided approach. Fluoroscopy time and fluoroscopy dose decreased significantly after 152 procedures. PVI-related minor complications occurred in 11 procedures (2.6%) and included 10 vascular-access-related complications (2.4%) and 1 TEE-related esophageal hematoma (0.2%), which healed spontaneously. Conclusion: Our single-center study shows that TEE guidance may allow safe transseptal access to the left atrium in patients undergoing PVI.
The Activity of Superoxide Dismutase, Its Relationship with the Concentration of Zinc and Copper and the Prevalence of rs2070424 Superoxide Dismutase Gene in Women with Polycystic Ovary Syndrome—Preliminary Study
Authors: Bizoń Anna , Tchórz Agata , Madej Paweł , Leśniewski Marcin , Wójtowicz Mariusz , Piwowar Agnieszka , Franik Grzegorz
Published: May 2022

Superoxide dismutase (SOD) is a crucial antioxidant enzyme involved in the antioxidant pathway during both normal cellular metabolism and different pathologic processes. We investigated the activity of the copper (Cu)-zinc (Zn) SOD1 as well as the level of Cu and Zn in the serum of women with polycystic ovary syndrome (PCOS) and control group. Moreover, the prevalence of rs2070424 gene polymorphism of the enzyme in the course of PCOS was evaluated. Significantly lower activity of SOD 1 and Cu, Zn concentration was found in the group of women with PCOS than without the syndrome. Insulin resistance in the group of women with PCOS caused a further SOD1 activity decrease, while Cu concentration and the value of Cu/Zn was increased when compared to women with normal insulin levels. Furthermore, we assessed for the first time the rs2070424 polymorphism of SOD1 in the women with PCOS, and in these patients we detected dominant variant AA (93.3%). Due to a small number of other genotypes, it is difficult to state if lower SOD1 activity was strictly associated with the AA variant or if other factors play a crucial role, but this should be taken into account.
From Plates to Baits: Using a Remote Video Foraging System to Study the Impact of Foraging on Fouling Non-Indigenous Species
Authors: Chebaane Sahar , Canning-Clode João , Ramalhosa Patrício , Belz Janina , Castro Nuno , Órfão Inês , Sempere-Valverde Juan , Engelen Aschwin Hillebrand , Pais Miguel Pessanha , Monteiro João Gama
Published: May 2022

Marinas are a gateway for the introduction and establishment of non-indigenous species (NIS). In these habitats, competition and predation are crucial determinants for NIS establishment and invasiveness. However, fish trophic preferences and biotic effects inside marinas are poorly known. This study proposes a novel method that combines the deployment of settlement plates to recruit different assemblages, followed by their use as bait in remote underwater video systems. This combined approach, addressed as a remote video foraging system (RVFS), can record fish foraging behaviour, including feeding choices and their impacts on fouling assemblage composition. An experimental RVFS trial carried out in a marina of Madeira Island, Portugal (NE Atlantic), identified the Mediterranean parrotfish, Sparisoma cretense, as the most important fouling grazer in the area. S. cretense behaved as a generalist and increased the heterogeneity of fouling assemblages, which can hamper NIS dominance of the fouling and reduce the pressure of propagules from the marina to the natural environment. The RVFS tool was useful to understand the trophic links between foragers and fouling and has the potential to provide relevant information for the management of NIS introductions, establishment and spread.
Coastal Protection Using Integration of Mangroves with Floating Barges: An Innovative Concept
Authors: Raju Rahul Dev , Arockiasamy Madasamy
Published: May 2022

Mangroves and moored barges are used individually for coastal protection and beach restoration. This conceptual paper discusses about the integration of mangroves with moored floating barges for coastal protection. The concept involves towing of a barge to a particular location, mooring it to the seafloor and planting mangroves along the shore or beach. The barges will be unmoored and towed away once the mangroves attain certain growth and are well rooted in the soil. Mangroves can protect the beach from incoming waves using their roots and branches. The incoming waves can be reduced by 50% to 99% using mangroves of 500 m width. Mangroves have a life span of 20–100 years, and they do not need any yearly maintenance as do any other conventional coastal protection measures. Mangroves are considered as soft coastal protection structures and are environmentally friendly. Mangroves will also improve the aesthetic appearance of the beach. This paper discusses about some of the research methodologies for the development of the barge-assisted mangroves coastal protection method. The dimensions of the barge, gap width between the moored barges and the environmental condition at the location determines the performance of the barge-assisted mangroves coastal protection method. The gap width between the barges, draft of the barge and breadth of the barge influence the resonant frequency of the fluid between the barges. The shielding effect of the floating barges can be used for other applications, such as berthing of ships and growing living shorelines using oysters, rocks, sand, plants, coir, etc. for coastal protection.
Spatial Distribution of Heavy Metals in Near-Shore Marine Sediments of the Jeddah, Saudi Arabia Region: Enrichment and Associated Risk Indices
Authors: Halawani Riyadh F. , Wilson Myra E. , Hamilton Kenneth M. , Aloufi Fahed A. , Taleb Md. Abu , Al-Zubieri Aaid G. , Quicksall Andrew N.
Published: May 2022

Red Sea coastal development has rapidly accelerated in recent decades that has led to a rise in the anthropogenic heavy metal levels in sediments. A total of 80 surficial sediment samples were collected from the shallow waters along the eastern Red Sea coast near Jeddah, Saudi Arabia. These samples were collected from three locations, designated as North, Middle and South of Jeddah, to assess the concentrations of six heavy metals: chromium (Cr), manganese (Mn), nickel (Ni), copper (Cu), zinc (Zn), and lead (Pb). The results showed that the concentrations (mg/kg) of these metals in the studied sediments follow this order: Pb (77.34 ± 150.59) > Mn (36.52 ± 37.72) > Zn (18.02 ± 23.94) > Cr (9.56 ± 5.81) > Cu (9.18 ± 13.67) > Ni (3.68 ± 4.54). The majority of the polluted sediments were recorded in the Middle and South locations. Pollution and enrichment indices such as Geo-accumulation indices (Igeo), Enrichment Factors (Ef), Contamination Factors (Cf), Pollution Load Indices (PLI), Potential Ecological Risk Indices (PERI) and Potential Toxicity Response Indices (RI) were calculated from the measured metals to establish baselines for the region and assess specific metal enrichments by location along the Jeddah coastline. The Igeo values showed that 30% of the Southern location stations are considered moderately to highly polluted. The Ef for all the studied sediments followed this order: Pb (extremely severe enrichment) > Zn > Cu > Cd > Cr (severe enrichment) > Ni (moderate enrichment).
Vocal Behaviour of a Bottlenose Dolphin Pod during a Deadly Bycatch Event in the Gulf of Catania, Ionian Sea
Authors: Terranova Francesca , Raffa Alessandra , Floridia Stefano , Monaco Clara , Favaro Livio
Published: May 2022

Cetacean bycatch is increasing worldwide and poses a threat to the conservation of several delphinids. The bottlenose dolphin (Tursiops truncatus) is frequently involved in bycatch incidents, due to its coastal distribution and opportunistic behaviour. The acoustic behaviour of cetaceans during death-related events is a largely unexplored topic. During an acoustic monitoring survey of the bottlenose dolphin population inhabiting the Gulf of Catania (Ionian Sea), we documented the entangling and subsequent death of a sub-adult male in a fishing net. Here, we provide a detailed analysis of the vocal behaviour of the pod of bottlenose dolphins during the event. We identified a total of 720 vocalisations in a timespan of 138 min, including 436 signature whistles, 51 non-signature whistles, and 233 burst pulses. We recorded high vocal activity, with a peak characterised by emission rates of 36 signature whistles per minute and 70 burst pulses per minute. Although future studies are required, our results show massive vocal activity during net entangling, characterised by a significant emission of signature whistles and burst pulses, including bray series of gulps and squeaks. Therefore, we suggest that developing tools for automatically detecting the peaks of these sound types could be helpful in recognising bycatch events, in order to better quantify the impact of professional fishing on small cetaceans.
Automatic Control and Routing of Marine Vessels
Authors: Sotnikova Margarita V.
Published: May 2022

Due to the intensive development of the global economy, many problems are constantly emerging connected with the safety of ships’ motion in the context of increasing marine traffic [...]
CRISPR/Cas9-Based Genome Editing and Its Application in Aspergillus Species
Authors: Jin Feng-Jie , Wang Bao-Teng , Wang Zhen-Dong , Jin Long , Han Pei
Published: May 2022

Aspergillus, a genus of filamentous fungi, is extensively distributed in nature and plays crucial roles in the decomposition of organic materials as an important environmental microorganism as well as in the traditional fermentation and food processing industries. Furthermore, due to their strong potential to secrete a large variety of hydrolytic enzymes and other natural products by manipulating gene expression and/or introducing new biosynthetic pathways, several Aspergillus species have been widely exploited as microbial cell factories. In recent years, with the development of next-generation genome sequencing technology and genetic engineering methods, the production and utilization of various homo-/heterologous-proteins and natural products in Aspergillus species have been well studied. As a newly developed genome editing technology, the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been used to edit and modify genes in Aspergilli. So far, the CRISPR/Cas9-based approach has been widely employed to improve the efficiency of gene modification in the strain type Aspergillus nidulans and other industrially important and pathogenic Aspergillus species, including Aspergillus oryzae, Aspergillus niger, and Aspergillus fumigatus. This review highlights the current development of CRISPR/Cas9-based genome editing technology and its application in basic research and the production of recombination proteins and natural products in the Aspergillus species.
Genotypic and Phenotypic Structure of the Population of Phytophthora infestans in Egypt Revealed the Presence of European Genotypes
Authors: El-Ganainy Sherif , Iqbal Zafar , Awad Hossam , Sattar Muhammad , Tohamy Abdel , Abbas Ahmed , Squires Julie , Cooke David
Published: May 2022

Late blight disease of potato and tomato, caused by Phytophthora infestans, results in serious losses to Egyptian and global potato and tomato production. To understand the structure and dynamics of the Egyptian population of P. infestans, 205 isolates were collected from potato and tomato plants during three growing seasons in 2010–2012. The characterization was achieved by mating-type assay, metalaxyl sensitivity assay, and virulence pattern. Additionally, genotyping of 85 Egyptian isolates and 15 reference UK isolates was performed using 12 highly informative microsatellite (SSR) markers and five effector (RxLR) genes. Mating-type testing showed that 58% (118 of 205) of the isolates belonged to mating type A1, 35% (71 isolates) to mating type A2, and the rest 8% (16 isolates) were self-fertile. The phenotype of metalaxyl response was represented as 45% resistant, 43% sensitive, and 12% as intermediate. Structure analysis grouped the 85 identified genotypes into two main clonal lineages. The first clonal lineage comprised 21 isolates belonging to A2 mating type and 8 self-fertile isolates. This clonal lineage was identified as Blue_13 or EU_13_A2. The second main clonal lineage comprised 55 isolates and was identified as EU_23_A1. A single isolate with a novel SSR genotype that formed a distinct genetic grouping was also identified. The effector sequencing showed good correspondence with the virulence data and highlighted differences in the presence and absence of loci as well as nucleotide polymorphism that affect gene function. This study indicated a changing population of P. infestans in Egypt and discusses the findings in the context of late blight management.
Species Diversity and Ecological Habitat of Absidia (Cunninghamellaceae, Mucorales) with Emphasis on Five New Species from Forest and Grassland Soil in China
Authors: Zhao Heng , Nie Yong , Zong Tong-Kai , Wang Yu-Jie , Wang Mu , Dai Yu-Cheng , Liu Xiao-Yong
Published: May 2022

Although species of Absidia are known to be ubiquitous in soil, animal dung, and insect and plant debris, the species diversity of the genus and their ecological habitats have not been sufficiently investigated. In this study, we describe five new species of Absidia from forest and grassland soils in southwestern China, with support provided by phylogenetic, morphological, and physiological evidence. The species diversity and ecological habitat of Absidia are summarized. Currently, 22 species are recorded in China, which mainly occur in soil, especially in tropical and subtropical forests and mountains. An updated key to the species of Absidia in China is also provided herein. This is the first overview of the Absidia ecological habitat.
Antifungal Potential of Azotobacter salinestris Strain Azt 31 against Phytopathogenic Fusarium spp. Associated with Cereals
Authors: Nagaraja Hanumanthu , Chennappa Gurikar , Deepa Nagaraj , Naik Manjunath Krishnappa , Ajithkumar Kadaiah , Amaresh Yatgal Sharanappa , Achar Premila N. , Sreenivasa M. Y.
Published: May 2022

Antifungal efficacy of Azotobacter salinestris against trichothecene-producing Fusarium spp. was investigated in maize, sorghum, and wheat. The three cereals were subjected to four treatments as control (T1), Fusarium alone (T2), combination of Fusarium and A. salinestris treatment (T3), and only A. salinestris (T4). All the treatments were evaluated for total mass of seedlings, root and shoot length, seed germination, and vigor index (VI), and extent of rhizoplane colonization by A. salinestris was investigated. Further, greenhouse studies were conducted to learn the efficacy of A. salinestris in vivo conditions. Antifungal efficacy was tested by the dual-culture method which resulted in significant reduction in Fusarium growth. Infection by Fusarium was reduced up to 50% in treated cereals such as maize, sorghum, and wheat, and there was also significant increase in seedling mass in the three hosts. Maize showed the highest VI (1859.715), followed by sorghum (1470.84), and wheat (2804.123) with A. salinestris treatment. In addition, seed germination was enhanced to 76% in maize, 69% in sorghum, and 68% in wheat, respectively. Efficacy of rhizoplane colonization showed successful isolation of A. salinestris with high CFU rate, and furthermore, significant colonization inhibition by Fusarium spp. was observed. In the greenhouse conditions, on the 45th day of the experimental set-up, the highest shoot length/root length recorded in maize was 155.70/70.0 cm, in sorghum 165.90/48.0 cm, and in wheat 77.85/56.0 cm, and the maximum root mass recorded was 17.53 g in maize, 4.52 g in sorghum, and 1.90 g in wheat. Our present study showed that seed treatment by A. salinestris, may be used as an alternate biocontrol method against Fusarium infection in maize, sorghum, and wheat.