bluuebunny/crossref_metadata_2025 huggingface dataset:
Does the Presence of Anxiety and Depression Symptoms Mediate the Association Between Family Functions and Self-Efficacy in Pregnant Women in the Third Trimester?: A Community-Based Cross-Sectional Survey
Authors: Wensu Zhou , Xidi Zhu , Shaojie Li , Baohua Zheng , Yunhan Yu , Huilan Xu , Zhao Hu , Xiyue Xiong
Published: November 2021

Objectives: Pregnant women in the third trimester can be more vulnerable to adverse mental health outcomes, but there is limited research on the association between family function and self-efficacy and potential mediation by symptoms of anxiety and depression.

Methods: The cross-sectional study enrolled 813 pregnant women in the third trimester from 14 communities of Hengyang city, Hunan province of China. All of the participants completed a battery of self-report measures of family function (Family Adaptation Partnership Growth and Resolve Index, APGAR-family), self-efficacy (General Self-Efficacy Scale, GSES), anxiety (measured by Generalized Anxiety Disorder scale with seven items, GAD-7), and depression symptoms (Patient Health Questionnaire with nine items, PHQ-9). The correlation analysis was performed using Spearman's correlation coefficient. Baron and Kenny's method and multiple mediation models with bootstrapping were used to determine whether the symptoms of anxiety and depression mediated the association between family functions and self-efficacy.

Results: There were 22.6% pregnant women in the low level of self-efficacy, with 60.9% in the moderate level and 16.5% of respondents in the high level. Self-efficacy had significant correlations with both anxiety symptoms (r = −0.19, p < 0.05), depression symptoms (r = −0.22, p < 0.05), and family function (r = 0.31, p < 0.05). Anxiety symptoms were significantly associated with self-efficacy (β = −0.016, p < 0.05). Depression symptoms were significantly associated with self-efficacy (β = −0.024, p < 0.05). Anxiety and depression symptoms partly mediated the association between family function and self-efficacy, accounting for 11.4 and 16.4% of total effect, respectively. It was indicated that pregnant women with a high degree of family function are less likely to have emotional symptoms and predicted to have higher levels of self-efficacy.

Conclusions: Anxiety and depression show mediating effects in the association between family function and self-efficacy. Improved family function can have a positive impact on pregnant women in the third trimester.
Evaluation of the Acceptability of a Prenatal Program for Women With Histories of Childhood Trauma: The Program STEP
Authors: Berthelot Nicolas , Drouin-Maziade Christine , Garon-Bissonnette Julia , Lemieux Roxanne , Sériès Thibaut , Lacharité Carl
Published: November 2021

Background: Childhood trauma would negatively affect pregnant women's mental health and would have intergenerational repercussions. However, there is a paucity of prenatal interventions specifically designed for women exposed to childhood trauma. The study aims to evaluate the acceptability of STEP, a manualized group intervention designed for pregnant women having experienced early life adversity.

Methods: The acceptability of STEP was assessed in four phases. In Phase 1, six experts evaluated whether the program activities were pertinent and trauma sensitive. In Phase 2, three parents read the intervention manuals and evaluated whether they considered each session relevant, interesting, and clear. In Phase 3, the program was briefly presented by phone to 309 pregnant women from the community. Women were inquired about their interest in the program, and the reasons for their lack of interest were assessed. In Phase 4, 30 pregnant women exposed to childhood trauma participated in the program and completed anonymous satisfaction questionnaires after each session. Psychological distress was also measured before and after the program.

Results: All activities were rated by independent experts as highly pertinent, adequate, and sufficiently safe to be offered to pregnant women. Parents who read through the intervention manuals also considered that the sessions were relevant, clear, and interesting. About half of the pregnant women from the community showed interest in the program. Participants reported very high levels of satisfaction and a significant decrease in psychological distress during the program.

Conclusions: Our findings show a high level of convergence among various indicators of program acceptability.
Mental Health and Integration: A Qualitative Study on the Struggles of Recently Arrived Refugees in Germany
Authors: Walther Lena , Rayes Diana , Amann Julia , Flick Uwe , Ta Thi Minh Tam , Hahn Eric , Bajbouj Malek
Published: November 2021

Introduction:Forcibly displaced people are at particular risk of mental health problems and also face specific integration challenges upon resettlement. Existing literature suggests that there may be a bidirectional relationship between mental health and integration. The present study seeks to understand the relationship between integration processes and mental health problems or significant negative emotional experiences among adult refugees in Germany.

Method:Applying a qualitative approach, we conducted 54 semi-structured interviews with refugees and asylum seekers who arrived in Germany between 2013 and 2018 currently residing in Berlin, Leipzig, or the Duisburg area in North Rhine-Westphalia. Data was collected between December 2018 and September 2019. We analyzed transcripts inductively using thematic analysis.

Results:Five themes covering the various links between integration and mental health problems or significant negative emotional experiences were identified. First, we found that the mental health consequences of past adverse experiences, as well as ongoing worries about those left behind in the homeland, can seriously impede refugees' ability to pursue activities key to integration. Second, the process of applying for and securing asylum can result in uncertainty and fear, which, in turn, burden the individual and may impact motivation for integration. Third, many of our participants described mental health ramifications related to feeling stuck and thwarted in the pursuit of building a life, especially in securing employment. Fourth, some participants described feeling so overwhelmed by fundamental tasks throughout the integration process, namely, language learning and bureaucratic processes, that these take a psychological toll. Fifth, we identified several forms of social disconnection between refugees and members of the host community due to xenophobia, social and cultural differences, physical and emotional isolation in refugee camps, as well as with co-nationals and fellow refugees. Negative emotions, mistrust, and socio-cultural differences that emerge throughout the integration processes seem to erode social cohesion among refugee communities, potentially further threatening mental health.

Conclusion:Mental health problems and integration processes appear to be closely related across different areas of integration. Innovative solutions to challenges identified by members of the refugee community in Germany stand to benefit mental health and integration outcomes simultaneously.
Interplay Between Adherence to the Mediterranean Diet and Lipid Profile: A Comparative Survey Between Day-Time Healthcare and Non-healthcare Female Workers
Authors: Di Lorenzo Luigi , Vimercati Luigi , Pipoli Antonella , Manghisi Nicola Mariano , Lampignano Luisa , Caputi Antonio , De Maria Luigi , Zupo Roberta , De Pergola Giovanni
Published: November 2021

Introduction: Occupational physicians, as an aspect of the periodic health surveillance of workers prescribed by law, must develop preventive programs against adverse health-related occurrences (Legislative Decree 81/2008, art.25) to reduce major risk factors for non-communicable/chronic diseases. Eating habits play an important role in defining risk trajectories in the workplace.

Methods: We randomly and cross-sectionally evaluated 147 females, of which 59 were healthcare workers (HCWs) and 88 were non-HCWs. The assessment included a dietary screening for adherence to the Mediterranean diet (MD) and a clinical baseline collection of major fluid biomarkers and anthropometric indicators for cardiovascular and metabolic risk.

Results: The HCW group exhibited greater adherence to the MD than the non-HCW group. Nevertheless, they showed higher serum levels of triglycerides and total cholesterol. Menopause and type of work significantly and unfavorably affected triglyceride serum levels among HCWs.

Conclusion: Greater preventive efforts are needed in the context of periodic health surveillance by occupational physicians. Disseminating additional information on a healthier lifestyle, particularly among female workers of perimenopausal age, is a key issue.
The Relationship Between Storey of Buildings and Fall Risk
Authors: Tsai Ching-Yao , Lin En-Sheng , Li Yang-Tzu , Tung Tao-Hsin , Chen Wei-Cheng
Published: November 2021

Purpose: This study examined the association between storey of building and fall risk in older adults' residences and residents' level of fear of falling.

Methods: The National Health and Ageing Trends Study (NHATS) collected information that would provide an understanding of basic trends people aged 65 years and older. Using a longitudinal survey, the present study employed the first round of NHATS data that was collected in 2011. In the first round, 12,411 participants were enrolled, and 8,077 interviews were completed. The study sample sizes for falling and worry about falling are 6,153 and 6,142, respectively.

Results: Unadjusted analysis revealed that storey of building was a risk factor for fall and worry about falling. There was a higher prevalence for fall and worry about falling when subjects lived in single storey of building compared with the subjects live in multi-storey. Logistic regression analysis showed no highly significant between storey of building and the fall/fear of falling.

Conclusion: Several clinical factors independently were indicated pertaining to the fall and worry about falling in older adult's residences.
Majority of Fortune 500 Companies in 2018 Did Not Recognize Risk of Epidemics Such as COVID-19
Authors: MacDonald Pia D. M. , Endres-Dighe Stacy M. , Macoubray Aaron J. , Shorey Jamie M.
Published: November 2021

Infectious disease threats, like the 2002 severe acute respiratory syndrome coronavirus (SARS-CoV) disease, 2009 pandemic influenza A (H1N1), and the current coronavirus disease 2019 (COVID-19), pose multisectoral risk with the potential for wide-ranging socioeconomic disruption. In our globally intertwined economy, the impact of such events can elicit economic shock waves that reach far beyond the country of origin. Review of the 2018 Fortune 500 company 10-K filings shows the majority did not document perceived risks associated with epidemics, outbreaks, or pandemics. Enhanced engagement and investment of the public and private sectors in advancing global health security is needed to effectively prevent, detect, and respond to infectious disease events and ensure U.S. economic security.
Life Cycle and Techno-Economic Assessment Templates for Emerging Carbon Management Technologies
Authors: Faber Grant , Mangin Christophe , Sick Volker
Published: November 2021

Technologies that valorize carbon dioxide are becoming an increasingly relevant component of the portfolio of solutions necessary to mitigate and reverse climate change. Assessing the environmental and economic characteristics of these technologies early in their developmental trajectories can help technologists either efficiently accelerate emissions reductions and commercialization or realize potential infeasibility and direct resources toward better opportunities. To aid in such assessments, this article constructs a typology of carbon removal and utilization technologies and identifies specific pathways in need of early-stage life cycle assessment (LCA) and techno-economic assessment (TEA) templates. Based on published literature and project experience, example LCA and TEA templates are developed for high-priority pathways with relatively low technology readiness levels including direct air capture, chemical synthesis, algae products, carbonated concrete, and carbonated aggregates. The templates attempt to capture the most important elements of early-stage LCA and TEA in an easily understandable and usable manner that still allows for reliable, order-of-magnitude estimations and hotspot analysis. Opportunities for other practitioners to use and build upon the templates are also discussed.
Spatiotemporal Variation Characteristics and Driving Factors of Nitrogen Use Efficiency of Wheat–Rice Rotation Systems in the Taihu Lake Region
Authors: Lu Xiaosong , Ma Lixia , Yu Dongsheng , Chen Yang , Wang Xin
Published: November 2021

During the past three decades, a large amount of nitrogen (N) fertilizers has been applied in the rice and wheat rotation system in the Taihu Lake region of southern China to achieve high yield, resulting in low N use efficiency (NUE). China is implementing the national strategy “fertilizer reduction with efficiency increase” to solve the serious ecological problems caused by excessive fertilization. However, the effects of N fertilizer reduction on soil fertility and their integrated effect on NUE of rice–wheat rotation systems in the Taihu Lake region are not fully understood. In this study, test fields with different soil-fertility qualities were selected in typical rice–wheat areas in the Taihu Lake region to perform a 2-year rice–wheat N fertilizer effect test to obtain the comprehensive quantitative relationship among the integrated fertility index (IFI), nitrogen application level (NA), and NUE. Through the investigation and spatial analysis of NA and IFI in the study area in 2003 and 2017, the spatial and temporal variation characteristics of NA and IFI in the study area in the past 15-year period were obtained, and this information was spatially coupled with the comprehensive quantitative relationship model of NUE to reveal the variation characteristics and driving factors of NUE in the study area. The result shows that the wheat and rice NA in the study area in 2017 increased by 35.5 and 8.4%, respectively, compared with 2003. Due to excessive fertilization, the soil nitrogen, phosphorus, and potassium content of cultivated land in the study area in 2017 was greater than that in 2003, especially soil-available phosphorus and potassium contents, whereas soil organic matter (SOM) content was reduced. The cultivated land IFI of the study area as a whole increased by 7.2% in the 15-year period. The NUE of rice and wheat rotation increased by 5.8% in 2017 compared with that of 2003 due to the improvement in crop varieties and N fertilizer yield benefits. The increases of NA and IFI both have negative correlations with the NUE improvement, and the NA increase has a greater impact. In addition, the terrain, soil type, texture, and parent material also affect the soil nutrient-preserving capability and, thus, affect the spatial variation of IFI and NUE improvement. These factors have greater influence on NUE improvement of wheat than rice. This study provides a novel and effective method for analyzing the spatial-temporal variation characteristics of NUE in the rice–wheat system and is conducive to guide precise fertilization and N fertilizer reduction based on the spatial analysis of NA with IFI and NUE.
Soil Health and Nutrient Density: Beyond Organic vs. Conventional Farming
Authors: Montgomery David R. , Biklé Anne
Published: November 2021

Controversy has long surrounded the question of nutritional differences between crops grown organically or using now-conventional methods, with studies dating back to the 1940s showing that farming methods can affect the nutrient density of crops. More recent studies have shown how reliance on tillage and synthetic nitrogen fertilizers influence soil life, and thereby soil health, in ways that can reduce mineral micronutrient uptake by and phytochemical production in crops. While organic farming tends to enhance soil health and conventional practices degrade it, relying on tillage for weed control on both organic and conventional farms degrades soil organic matter and can disrupt soil life in ways that reduce crop mineral uptake and phytochemical production. Conversely, microbial inoculants and compost and mulch that build soil organic matter can increase crop micronutrient and phytochemical content on both conventional and organic farms. Hence, agronomic effects on nutritional profiles do not fall out simply along the conventional vs. organic distinction, making the effects of farming practices on soil health a better lens for assessing their influence on nutrient density. A review of previous studies and meta-studies finds little evidence for significant differences in crop macronutrient levels between organic and conventional farming practices, as well as substantial evidence for the influence of different cultivars and farming practices on micronutrient concentrations. More consistent differences between organic and conventional crops include that conventional crops contain greater pesticide levels, whereas organically grown crops contain higher levels of phytochemicals shown to exhibit health-protective antioxidant and anti-inflammatory properties. Thus, part of the long-running controversy over nutritional differences between organic and conventional crops appears to arise from different definitions of what constitutes a nutrient—the conventional definition of dietary constituents necessary for growth and survival, or a broader one that also encompasses compounds beneficial for maintenance of health and prevention of chronic disease. For assessing the effects of farming practices on nutrient density soil health adds a much needed dimension—the provisioning of micronutrients and phytochemicals that support human health.
Prevalence of Newcastle Disease Virus in Commercial and Backyard Poultry in Haryana, India
Authors: Joshi Vinay G. , Chaudhary Deepika , Bansal Nitish , Singh Renu , Maan Sushila , Mahajan Nand K. , Ravishankar Chintu , Sahoo Niranjana , Mor Sunil K. , Radzio-Basu Jessica , Herzog Catherine M. , Kapur Vivek , Goel Parveen , Jindal Naresh , Goyal Sagar M.
Published: November 2021

Newcastle disease virus (NDV) causes Newcastle disease (ND) in poultry. The ND is a highly contagious disease, which is endemic in several countries despite regular vaccination with live or killed vaccines. Studies on NDV in India are mostly targeted toward its detection and characterization from disease outbreaks. A surveillance study was undertaken to determine NDV prevalence throughout the state of Haryana from March 2018 to March 2020 using a stratified sampling scheme. The state was divided into three different zones and a total of 4,001 choanal swab samples were collected from backyard poultry, commercial broilers, and layers. These samples were tested for the M gene of NDV using real-time RT-PCR. Of the 4,001 samples tested, 392 were positive (9.8% apparent prevalence; 95% CI: 8.9–10.8%) for the M gene. Of these 392 M gene positive samples, 35 (8.9%; 95% CI: 6.4–12.3%) were found to be positive based on F gene real-time RT-PCR. Circulation of NDV in commercial and backyard poultry highlights the importance of surveillance studies even in apparently healthy flocks. The information generated in this study should contribute to better understanding of NDV epidemiology in India and may help formulate appropriate disease control strategies for commercial and backyard birds.
Case Report: Radioactive Holmium-166 Microspheres for the Intratumoral Treatment of a Canine Pituitary Tumor
Authors: Morsink Nino Chiron , Klaassen Nienke Johanna Maria , Meij Björn Petrus , Kirpensteijn Jolle , Grinwis Guillaume Cornelis Maria , Schaafsma Irene Afra , Hesselink Jan Willem , Nijsen Johannes Frank Wilhelmus , van Nimwegen Sebastiaan Alexander
Published: November 2021

Introduction: In this case study, a client-owned dog with a large pituitary tumor was experimentally treated by intratumoral injection of radioactive holmium-166 microspheres (166HoMS), named 166Ho microbrachytherapy. To our knowledge, this is the first intracranial intratumoral treatment through needle injection of radioactive microspheres.

Materials and Methods: A 10-year-old Jack Russell Terrier was referred to the Clinic for Companion Animal Health (Faculty of Veterinary Medicine, Utrecht University, The Netherlands) with behavioral changes, restlessness, stiff gait, and compulsive circling. MRI and CT showed a pituitary tumor with basisphenoid bone invasion and marked mass effect. The tumor measured 8.8 cm3 with a pituitary height-to-brain area (P/B) ratio of 1.86 cm−1 [pituitary height (cm) ×10/brain area (cm2)]. To reduce tumor volume and neurological signs, 166HoMS were administered in the tumor center by transsphenoidal CT-guided needle injections.

Results: Two manual CT-guided injections were performed containing 0.6 ml of 166HoMS suspension in total. A total of 1097 MBq was delivered, resulting in a calculated average tumor dose of 1866 Gy. At 138 days after treatment, the tumor volume measured 5.3 cm3 with a P/B ratio of 1.41 cm−1, revealing a total tumor volume reduction of 40%. Debulking surgery was performed five months after 166HoMS treatment due to recurrent neurological signs. The patient was euthanized two weeks later at request of the owners. Histopathological analysis indicated a pituitary adenoma at time of treatment, with more malignant characteristics during debulking surgery.

Conclusion: The 40% tumor volume reduction without evident severe periprocedural side effects demonstrated the feasibility of intracranial intratumoral 166HoMS treatment in this single dog.
Cervical Epidural Spinal Analgesia for Acute Management of Severe Unilateral Forelimb Lameness: Case Report
Authors: Watkins Amanda R. , Hopster Klaus , Levine David , Hurcombe Samuel D.
Published: November 2021

A 20-year-old Quarter Horse gelding was presented with severe right forelimb lameness (5/5 AAEP Lameness Scale) due to a tear of the superficial digital flexor muscle which was diagnosedviapalpation of swelling and ultrasonography revealing major muscle fiber disruption and hematoma formation. When traditional systemic therapy (non-Steroidal anti-inflammatories) did not restore clinically acceptable comfort and the risk of supporting limb laminitis became a reasonable concern, a cervical epidural catheter was placed between the first and second cervical vertebrae in the standing, sedated patient using ultrasound guidance. The gelding was treated with epidural morphine (0.1 mg/kg every 24 h then decreased to 0.05 mg/kg every 12 h) and was pain-scored serially following treatment. Spinal analgesia was provided for 3 days. Pain scores significantly decreased following each treatment with morphine, and the gelding was successfully managed through the acutely painful period without any adverse effects associated with the C1-C2 epidural catheter placement technique, the epidural morphine, or contralateral limb laminitis. At the 2-month follow-up, the gelding was walking sound with no complications seen at the catheter insertion site. In this case, spinal analgesia using epidural morphine administeredviaa cervical epidural catheter was an effective and technically achievable option for pain management associated with severe forelimb muscle injury in a horse.
Subclinical Hypoventilation in Dogs Undergoing Ventral Slot Decompressive Surgery for Cervical Myelopathy Due to Intervertebral Disc Herniation
Authors: Andruzzi Melissa N. , Simon Bradley T. , Boudreau Elizabeth
Published: November 2021

The objective of this prospective cohort study was to document the occurrence of post-operative hypoventilation in dogs undergoing decompressive ventral slot or hemilaminectomy for the treatment of intervertebral disc herniation (IVDH). Twenty dogs undergoing ventral slot surgery and 20 dogs undergoing hemilaminectomy surgery for the treatment of IVDH that presented to XX between 2017 and 2020 were enrolled. Dogs were anesthetized using a standard protocol. Blood gas samples were taken at up to 11 time points beginning during anesthetic recovery and continuing for a maximum of 72 h post-operatively. Dogs with cervical lesions that were non-ambulatory before surgery had more evidence of subclinical hypoventilation in the immediate peri-extubation period than dogs with less severe injuries or those undergoing hemilaminectomy surgery. We found no difference in the ventilation status in dogs undergoing cervical or thoracolumbar decompressive surgery for IVDH from 8 to 72 h post-operatively. Other markers of acid-base status indicated that subclinical hypoventilation within the peri-extubation period was transient and self-limiting. There was a moderate positive correlation between sedation scores and estimated PaCO2. These data suggest that dogs with severe cervical spinal cord injuries may be at risk for subclinical hypoventilation in the immediate peri-extubation period. Increased sedation may be correlated with decreased ventilatory status in dogs recovering from decompressive vertebral column surgery.
Factors Influencing the Adoption of Agricultural Machinery by Chinese Maize Farmers
Authors: Quan Xiuhao , Doluschitz Reiner
Published: November 2021

As the major labor force has shifted from rural areas to cities, labor shortages in agricultural production have resulted. In the context of technical progress impact, and depending on farm resource endowments, farmers will choose effective labor saving technology such as machinery to substitute for the missing manual labor. The reasons behind farmers’ adoption of machinery technology are worth exploring. Therefore, this study uses 4165 Chinese maize farmers as the target group. Multivariate probit models were performed to identify the factors that affect maize farmers’ adoption of four machinery technologies as well as the interrelation between these adoption decisions. The empirical results indicate that maize sowing area, arable land area, crop diversity, family labor, subsidy, technical assistance, and economies of scale have positive effects on machinery adoption, while the number of discrete fields in the farm has a negative impact. Maize farmers in the Northeast and North have higher machinery adoption odds than other regions. The adoption of these four machinery technologies are interrelated and complementary. Finally, moderate scale production, crop diversification, subsidizing agricultural machinery and its extension education, and land consolidation, are given as recommendations for promoting the adoption of agricultural machinery by Chinese maize farmers.
Bioavailability of Dietary Zinc Sources and Their Effect on Mineral and Antioxidant Status in Lambs
Authors: Grešáková Ľubomíra , Tokarčíková Katarína , Čobanová Klaudia
Published: November 2021

This study investigated the relative bioavailability (RBV) of zinc from different sources used as feed additives in ruminant nutrition based on Zn concentration and the activity of Zn-dependent enzymes in lamb tissues. Thirty-two male lambs of Improved Valachian breed (three months old) were randomly assigned to four dietary treatments. For 120 days, the lambs were fed either the total mix ration (TMR) providing 29.6 mg Zn/kg or the TMR supplemented with either zinc sulphate (ZnSO4), zinc chelate of glycine hydrate (ZnGly), or zinc chelate of protein hydrolysate (ZnProt). The supplemented diets contained a total of 80 mg Zn/kg. Supplementation with ZnSO4 increased Zn concentration in the liver, while the highest Zn uptake was in the kidneys of lambs fed the ZnProt diet. The ZnGly supplemented diet elevated the activity of the Cu/Zn-dependent enzyme superoxide dismutase (Cu/Zn SOD) in the liver. Regardless of Zn source, Zn supplementation resulted in increased total antioxidant status (TAS) in the pancreas. The estimated RBV of Zn based on linear regression slope ratios did not differ among the Zn sources. Our results indicate similar availability of Zn from organic dietary sources as from commonly used zinc sulphate; however, their effects on mineral and antioxidant status may differ slightly in growing lambs.
Human Waste Substitute Strategies Enhanced Crop Yield, Crop Quality, and Soil Fertility in Vegetable Cultivation Soils in North China
Authors: Liu Bo , Yang Bo , Zhang Chunxue , Wei Xiaocheng , Cao Haoyu , Zheng Xiangqun
Published: November 2021

Replacing chemical fertilizers with human waste for vegetable planting is a traditional, economical, and environmentally friendly waste resource utilization strategy. However, whether the human waste substitute strategy can improve soil fertility and increase crop yield and quality compared to the simple application of chemical fertilizers is still unclear, especially under acidic and alkaline soil conditions. In this study, we studied the effects of different ratios of human waste (urine and feces) to chemical fertilizer on the crop yield, crop quality, soil fertility, and soil chemical parameters in alkaline Cambisols and acidic Alisols cultivated with water spinach (Ipomoea aquatica Forssk.). The application variants of human waste and chemical fertilizer were as follows: (i) Control, no fertilization (CK), (ii) human waste application (HW), (iii) chemical fertilizer application (CF), (iv) 1/3 human waste to chemical fertilizer (P1), and (v) 2/3 human waste to chemical fertilizer (P2). Human waste application increased the total nitrogen, available phosphorus, available potassium, organic matter, NO3−-N, and conductivity in soil, enhanced soil enzyme activity, slowed down soil acidification, and increased the yield, soluble sugar, and vitamin C contents of the water spinach while reducing its nitrate content. Our findings indicate that human waste substitution improved soil fertility while reducing the potential risks of soil acidification, salinization, and human exposure to nitrates. These findings may be applied to increase vegetable production and quality, improve the soil environment, and increase the utilization of human waste as a valuable resource.
Fused-Deep-Features Based Grape Leaf Disease Diagnosis
Authors: Peng Yun , Zhao Shengyi , Liu Jizhan
Published: November 2021

Rapid and accurate grape leaf disease diagnosis is of great significance to its yield and quality of grape. In this paper, aiming at the identification of grape leaf diseases, a fast and accurate detection method based on fused deep features, extracted from a convolutional neural network (CNN), plus a support vector machine (SVM) is proposed. In the research, based on an open dataset, three types of state-of-the-art CNN networks, three kinds of deep feature fusion methods, seven species of deep feature layers, and a multi-class SVM classifier were studied. Firstly, images were resized to meet the input requirements of the CNN network; then, the deep features of the input images were extracted via the specific deep feature layer of the CNN network. Two kinds of deep features from different networks were then fused using different fusion methods to increase the effective classification feature information. Finally, a multi-class SVM classifier was trained with the fused deep features. The experimental results on the open dataset show that the fused deep features with any kind of fusion method can obtain a better classification performance than using a single type of deep feature. The direct concatenation of the Fc1000 deep feature extracted from ResNet50 and ResNet101 can achieve the best classification result compared with the other two fusion methods, and its F1 score is 99.81%. Furthermore, the SVM classifier trained using the proposed method can achieve a classification performance comparable to that of using the CNN model directly, but the training time is less than 1 s, which has an advantage over spending tens of minutes training a CNN model. The experimental results indicate that the method proposed in this paper can achieve fast and accurate identification of grape leaf diseases and meet the needs of actual agricultural production.
Potential Role and Involvement of Antioxidants and Other Secondary Metabolites of Wheat in the Infection Process and Resistance to Fusarium spp.
Authors: Chrpová Jana , Orsák Matyáš , Martinek Petr , Lachman Jaromír , Trávníčková Martina
Published: November 2021

This article provides a summary of current knowledge about wheat metabolites that may affect resistance against Fusarium head blight (FHB). The mechanisms of resistance, the roles of secondary metabolites in wheat defense, and future directions for breeding are assessed. The soluble phenols play an important role in redox regulation in plant tissues and can act as antimicrobial compounds. The color of cereal hulls and grains is caused by such natural pigments as anthocyanins in the aleurone, endosperm, and pericarp layers of the grain. Phenolic acids, alkylresorcinols, and phytohormones actively participate in the defense system, whereas carotenoids show various effects against Fusarium species that are positively correlated with the levels of their mycotoxins. Pathogen infestation of vegetative tissues induces volatile organic compounds production, which can provide defensive functions to infested wheat. The efficient use of native resistance in the wheat gene pool, introgression of resistant alleles, and implementation of modern genotypic strategies to increase levels of native secondary metabolites with antifungal properties can enhance the FHB resistance of new varieties. Expanding the breeding interest in the use of forms with different grain color and plant organs can be a potential benefit for the creation of lines with increased resistance to various stresses.
Influence of Essential Oils on the Microbiological Quality of Fish Meat during Storage
Authors: Kunová Simona , Sendra Esther , Haščík Peter , Vukovic Nenad L. , Vukic Milena , Kačániová Miroslava
Published: November 2021

The aim of the present study was to evaluate the microbiological quality of rainbow trout meat treated with essential oils (EOs from Citrus limon and Cinnamomum camphora) at concentrations of 0.5% and 1.0% in combination with vacuum packaging during storage. The composition of the EOs were analyzed by gas chromatography coupled with mass spectrometry, and total viable counts (TVCs), coliform bacteria (CB), and lactic acid bacteria (LAB) were determined on the zeroth, first, third, fifth, and seventh days of storage at 4 °C. Individual species of isolated microorganisms were identified using a MALDI-TOF MS Biotyper. The results show that the major components of the EOs were linalool (98.1%) in C. camphora and α-limonene in C. limon. The highest number of TVCs and CB were 4.49 log CFU/g and 2.65 log CFU/g in aerobically packed samples at the seventh day. The lowest TVCs were those of samples treated with 1% C. camphora EO. For CB the most effective treatment was 1% lemon EO. LAB were only detected in a few samples, and were never present in aerobically packed samples; the highest number of LAB was 1.39 log CFU/g in samples treated with 1% lemon EO at day seven. The most commonly isolated coliform bacteria were Hafnia alvei, Serratia fonticola, Serratia proteamaculans, Pantoea agglomerans, and Yersinia ruckeri. Lactobacillus sakei, Staphylococcus hominis, and Carnobacterium maltaromaticum were the most frequently isolated bacteria from lactic acid bacteria. In conclusion, C. camphora EO at a concentration of 1% showed the highest antimicrobial activity.
Sex Differences in Mouse Exploratory Behaviour to Fel d 1, a Cat ABP-Like Protein
Authors: Grau Carlos , Bienboire-Frosini Cécile , Arroub Sana , Lafont-Lecuelle Céline , Leclercq Julien , Pageat Patrick
Published: November 2021

Fel d 1 is a cat protein abundantly released and found in their habitat and is closely related to mouse androgen-binding proteins (ABPs). We hypothesized that mice have developed chemical communication mechanisms to detect and avoid this protein. We tested purified natural Fel d 1, a fox faeces molecule (TMT) as a positive control, and a negative control (purified water) in three different mouse groups (n = 14 each) to evaluate exploratory behaviour and stress responses. The mice did not show clear avoidance or stress responses to Fel d 1. Our results demonstrated a sex-treatment interaction for Fel d 1, with males spending more time in the areas treated with Fel d 1 than in the untreated areas (p = 0.018). This sex-treated area interaction was also not observed for either the blank or TMT. These results suggest that Fel d 1 from domestic cats could be recognized differently by male and female mice. These sex differences could be linked to the sexual role of ABP proteins and the ABP-like characteristics of Fel d 1.
Invasion Risk and Potential Impact of Alien Freshwater Fishes on Native Counterparts in Klang Valley, Malaysia
Authors: Saba Abdulwakil Olawale , Ismail Ahmad , Zulkifli Syaizwan Zahmir , Ghani Intan Faraha A. , Halim Muhammad Rasul Abdullah , Ibrahim Musa Adamu , Mukhtar Aqilah , Aziz Azharuddin Abd , Wahid Noor Azrizal Abdul , Amal Mohammad Noor Azmai
Published: November 2021

This study explores the potential effects of alien fishes on the native fish community, well-being, and tropic preferences in selected rivers of Klang Valley, Malaysia. Following the Aquatic Species Invasiveness Screening Kit assessment, most of the alien fishes (80%) are invasive. The alien species occurrences correlated positively (p < 0.05) with poor water quality, such as rivers with high ammonia-nitrogen and nitrite, but negatively with phosphate and dissolved oxygen. Anthropogenic characteristics, such as rivers with high pollution levels and ease of accessibility to the fish habitat, are mainly associated positively (p < 0.05) with the occurrences of alien fish species. In general, the results of fish stomach contents analyses and their associated indices, together with stable carbon and nitrogen isotopes, revealed domination by alien fishes or diet overlaps between both alien and native fish species. This finding indicates that alien fishes benefited from the impacts of the anthropogenic activities in their surrounding habitats, while their plasticity in feeding habits might help them to invade, survive, and dominate in the rivers of Klang Valley, Malaysia.
Donkey Ownership Provides a Range of Income Benefits to the Livelihoods of Rural Households in Northern Ghana
Authors: Maggs Heather C. , Ainslie Andrew , Bennett Richard M.
Published: November 2021

Donkeys provide important resources and benefits for millions of people worldwide. However, global donkey populations are under increasing pressure from the growing demand for a traditional Chinese medicine, e’jiao, made from donkey-skin. The objective of this reflexive, qualitative thematic analysis was to examine the role of donkeys with 262 participants in northern Ghana and how donkeys contribute to livelihood outcomes, especially their use by women and children. Data were collected from four surveys, 12 in-depth interviews and 84 daily time budgets with the same participants, plus 16 focus groups, during one wet and one dry season across 2018-19. Uniquely, boys and girls between the ages of 10–16-years old were interviewed. Donkeys are highly valued by their owners as they play a valuable role in providing a pathway out of ultra-poverty. Donkeys’ contributions to livelihoods are significant and more complex than previously understood and documented in the literature. Donkey ownership confers up to six different income benefits in comparison to non-donkey owners. Female owners of donkeys reported that donkeys can contribute between 30–60% of their income. Children of both sexes can play an important role in the efficient deployment of one of these income generating activities.
Use of Antibiotics among Residents Living Close to Poultry or Goat Farms: A Nationwide Analysis in The Netherlands
Authors: Roof Inge , van der Hoek Wim , Oude Boerrigter Lisette , Wielders Cornelia C. H. , Smit Lidwien A. M.
Published: November 2021

Prior regional studies found a high risk of pneumonia for people living close to poultry and goat farms. This epidemiological study in the Netherlands used nationwide antibiotic prescription data as a proxy for pneumonia incidence to investigate whether residents of areas with poultry and goat farms use relatively more antibiotics compared to areas without such farms. We used prescription data on antibiotics most commonly prescribed to treat pneumonia in adults and livestock farming data, both with nationwide coverage. Antibiotic use was expressed as defined daily doses per (4-digit Postal Code (PC4) area)-(age group)-(gender)-(month) combination for the year 2015. We assessed the associations between antibiotic use and farm exposure using negative binomial regression. The amoxicillin, doxycycline, and co-amoxiclav use was significantly higher (5–10% difference in use) in PC4 areas with poultry farms present compared to areas without, even after adjusting for age, gender, smoking, socio-economic status, and goat farm presence. The adjusted models showed no associations between antibiotic use and goat farm presence. The variables included in this study could only partly explain the observed regional differences in antibiotic use. This was an ecological study that precludes inference about causal relations. Further research using individual-level data is recommended.
Novel Photoinduced Squalene Cyclic Peroxide Identified, Detected, and Quantified in Human Skin Surface Lipids
Authors: Khalifa Saoussane , Enomoto Masaru , Kato Shunji , Nakagawa Kiyotaka
Published: November 2021

Skin surface lipids (SSLs) form the first barrier that protects the human organism from external stressors, disruption of the homeostasis of SSLs can result in severe skin abnormalities. One of the main causes of this disruption is oxidative stress that is primarily due to SSLs oxidation. Squalene (SQ), the most abundant lipid among SSLs, was shown to first undergo singlet molecular oxygen (1O2) oxidation to yield 6 SQ-monohydroperoxide (SQ-OOH) isomers as the primary oxidation products. However, due to the instability and lability of hydroperoxides, we found that when total SQ-OOH isomers are further photooxidized, they form a unique higher molecular weight secondary oxidation product. To generate the compound, we photooxidized total SQ-OOH isomers in the presence of ground state molecular oxygen (3O2), after its isolation and purification, we studied its structure using MS/MS, NMR, derivatization reactions, and chemical calculations. The compound was identified as 2-OOH-3-(1,2-dioxane)-SQ. Photooxidation of individual SQ-OOH isomers revealed that 6-OOH-SQ is the precursor of 2-OOH-3-(1,2-dioxane)-SQ and indicated the possibility of the formation of similar cyclic peroxides from each isomer following the same photoinduced chain reaction mechanism. An HPLC-MS/MS method was developed for the analysis of 2-OOH-3-(1,2-dioxane)-SQ and its presence on the skin was confirmed in SSLs of six healthy individuals. Its quantity on the skin correlated directly to that of SQ and was not inversely proportional to its precursor, indicating the possibility of its accumulation on the skin surface and the constant regeneration of 6-OOH-SQ from SQ’s oxidation. In general, research on lipid cyclic peroxides in the human organism is very limited, and especially on the skin. This study shows for the first time the identification and presence of a novel SQ cyclic peroxide “2-OOH-3-(1,2-dioxane)-SQ” in SSLs, shedding light on the importance of further studying its effect and role on the skin.
Experimental and Numerical Analysis of 3D Printed Polymer Tetra-Petal Auxetic Structures under Compression
Authors: Photiou Demetris , Avraam Stelios , Sillani Francesco , Verga Fabrizio , Jay Olivier , Papadakis Loucas
Published: November 2021

Auxetic structures possess a negative Poisson ratio (ν < 0) as a result of their geometrical configuration, which exhibits enhanced indentation resistance, fracture toughness, and impact resistance, as well as exceptional mechanical response advantages for applications in defense, biomedical, automotive, aerospace, sports, consumer goods, and personal protective equipment sectors. With the advent of additive manufacturing, it has become possible to produce complex shapes with auxetic properties, which could not have been possible with traditional manufacturing. Three-dimensional printing enables easy and precise control of the geometry and material composition of the creation of desirable shapes, providing the opportunity to explore different geometric aspects of auxetic structures with a variety of different materials. This study investigated the geometrical and material combinations that can be jointly tailored to optimize the auxetic effects of 2D and 3D complex structures by integrating design, modelling approaches, 3D printing, and mechanical testing. The simulation-driven design methodology allowed for the identification and creation of optimum auxetic prototype samples manufactured by 3D printing with different polymer materials. Compression tests were performed to characterize the auxetic behavior of the different system configurations. The experimental investigation demonstrated a Poisson’s ration reaching a value of ν = −0.6 for certain shape and material combinations, thus providing support for preliminary finite element studies on unit cells. Finally, based on the experimental tests, 3D finite element models with elastic material formulations were generated to replicate the mechanical performance of the auxetic structures by means of simulations. The findings showed a coherent deformation behavior with experimental measurements and image analysis.
The Usefulness of Video Learning Analytics in Small Scale E-Learning Scenarios
Authors: Córcoles César , Cobo Germán , Guerrero-Roldán Ana-Elena
Published: November 2021

A variety of tools are available to collect, process and analyse learning data obtained from the clickstream generated by students watching learning resources in video format. There is also some literature on the uses of such data in order to better understand and improve the teaching-learning process. Most of the literature focuses on large scale learning scenarios, such as MOOCs, where videos are watched hundreds or thousands of times. We have developed a solution to collect clickstream analytics data applicable to smaller scenarios, much more common in primary, secondary and higher education, where videos are watched tens or hundreds of times, and to analyse whether the solution is useful to teachers to improve the learning process. We have deployed it in a real scenario and collected real data. Furthermore, we have processed and presented the data visually to teachers for those scenarios and have collected and analysed their perception of their usefulness. We conclude that the collected data are perceived as useful by teachers to improve the teaching and learning process.