bluuebunny/crossref_metadata_2025 huggingface dataset:
Postpartum Mother's Knowledge about the use of Postpartum Contraceptive
Authors: Muthoharoh Husnul
Published: May 2022

Most Mothers have difficulty in determining the exact type of contraception in the puerperium, because the mother has not previously understood about contraception to be used, this study aims to determine the post-partum mothers knowledge about contraception in the puerperium in Dlanggu Village, Kec. Deket, Kab. Lamongan.Design used in this study is descriptive Using a population of 30 respondents, the sample of 30 people with positive sampling techniques and analysis of the questionnaire as a measuring tool used is the frequency and distribution of research results obtained are good enough, lacking.The study found almost half of respondents who have sufficient knowledge about contraception is 14 (46.7%) respondents, and almost half again the respondents had less knowledge about contraception in the puerperium of 11 (36.7%) respondents.Conclusionof this study was almost half a per cent of respondents knowledgeable enough about contraception. So health officials are expected to conduct counseling to assist clients in selecting and deciding the type of contraception that will be used according to choice.Keywords: Contraceptive; Konowledge; Postpartum
Pencegahan Penularan Covid-19 dalam Al Quran Surat Al Baqarah, Al Maidah, dan Al A’raf
Authors: Ananta Favian Rakha , Putra Wahyu Anggoro
Published: May 2022

Wabah Corona Virus Disease atau lebih dikenal dengan nama virus corona atau COVID-19 yang pertama kali terdeteksi muncul di Cina tepatnya di Kota Wuhan, Tiongkok. Pada akhir tahun 2019, mendadak menjadi teror mengerikan bagi masyarakat dunia, terutama setelah merenggut nyawa ratusan orang dalam waktu yang relatif singkat. Hampir kurang lebih 215 Negara di Dunia terjangkit virus corona termasuk Indonesia. Berbagai upaya dalam rangka pencegahan, pengobatan dan sebagainya pun telah dilakukan dalam mencegah penyebaran virus corona, hingga lockdown dan social distancing di kota-kota besar sudah dilakukan untuk memutus rantai penyebaran virus corona. Dalam Islam wabah virus corona ini merupakan sebuah ujian bagi suatu kaum agar selalu mendekatkan diri kepada Allah SWT. Al-Quran yang merupakan kitab suci umat Islam telah memberikan petunjuk dalam menghadapi penyakit ini.Kata kunci: Pencegahan, covid-19, pencegahan covid-19 dalam al-quran
Gambaran Karakteristik Penderita HIV/AIDS di Kabupaten Sumenep Tahun 2018-2019
Authors: Mafluhah Luluk Rofiatul , Aboes Sjakoer Nour Athiroh , Lisminingsih Ratna Djuniwati
Published: May 2022

HIV/AIDS cases have high rates in various countries. The reason is, HIV/AID cases are like an iceberg phenomenon, cases that are not seen and not recorded are more than cases that have been recorded. This study aims to describe the characteristics of HIV/AIDS sufferers in Sumenep District. This study used a descriptive qualitative method with a cross-sectional research design. There were 202 HIV/AIDS sufferers in Sumenep District, while the sample taken was 44 people from 3 Public Health Center. The results showed that based on gender the most were male (57.95%), while the age range was 25-49 years (75.25%), and the area with the most sufferers was Kalianget Sub-District (10.4%) of a total 202 sufferers. Referring to 44 patients who were sampled in this study, there were 39 patients (88.6%) who were married, the most transmission was sex (90.9%), most opportunistic infections were pulmonary tuberculosis (20.4%). Stage I (63.6%) is the most common clinical-stage suffered, and most HIV/AIDS sufferers have been infected for 1-2 years (93.2%). To break the chain of HIV/AIDS transmission, the public needs to be educated, from the mode of transmission to treatment, this also aims to eliminate the negative stigma against people with HIV/AIDS.Keywords: HIV/AIDS, Characteristics, Sumenep
Studi In silico Potensi Anti Hipertensi dan Prediksi Profil Farmakokinetika Daun Jati Belanda (Guazuma ulmifolia. Lamk)
Authors: Tilaqza Andri , Herbani Merlita
Published: May 2022

Abstract. Hypertension is a disease that is often occurred in every country, including Indonesia. Hypertension is one of the risk factors that lead to cardiovascular disease. Guazuma ulmifolia Lamk are one of the plants used empirically as antihypertensive in Indonesia, but research on the antihypertensive potential of its leaves has never been done before.The purpose of this study was to predict the potential activity and predict the pharmacokinetic of several compounds contained in Guazuma ulmifolia leaves. The Guazuma ulmifolia leaves compounds and captopril were docked to the Angiotensin-Converting Enzyme protein (PDB ID: 1O86). Mole­cular docking tests were performed using PyRx software while the visuali­zation analysis of molecular anchoring results was performed using Biovia Discovery Studio Visualizer software. Prediction of phar­macokinetic and physicochemical properties of the tested compounds was carried out using the pkCSM web page. Based on the molecular docking test, all compounds in Guazuma ulmifolia leaves have lower energy than the captopril. Kaempferitrin (-9.9 k/mol) and beta-sitosterol (-9.2 kcal/mol) had the lowest energy among other test compounds compared to captopril (-5.5 kcal/mol). Quercetin, kaempferol, and beta-caryophyllene were compounds that had similar interactions to the captopril based on the chemical interaction. All compounds had varied pharmacokinetic profiles but still had a fairly good profile.
Hubungan Pengetahuan tentang Penggunaan OAD pada Pasien Diabetes Mellitus Tipe 2 terhadap Kepatuhan Penggunaan Obat Antidiabetes Oral di Puskesmas Singosari Malang
Authors: Faramida Sari Niken , Ratna Hidayati Ika , Novia Atmadani Rizka
Published: May 2022

Abstract. Background: Diabetes Mellitus is a chronic disease characterized by high blood sugar levels in the body and can be caused by the pancreas not being able to produce enough insulin or because the cells in the body cannot respond to the insulin that has been produced. Management includes diet, physical activity, and administration of insulin injections and oral antidiabetic drugs (OAD). The objective of this study is to determine the relationship between knowledge of Type 2 Diabetes Mellitus patients and adherence to oral antidiabetic drug use at Singosari Public Health Center, Malang Regency. Research Methods: This research is analytic observational with a cross-sectional approach. Conducted in July 2021 at the Singosari Public Health Center, Malang Regency, by distributing questionnaires to 66 Type 2 Diabetes patients who met the inclusion criteria. The analysis was carried out with the SPSS application using descriptive and analytic methods. The results of the validity and reliability tests have met the requirements Result and Discussion:. Patients who have good knowledge of 74.2%, 18.2% good knowledge, and 7.6% poor knowledge. Patients with good knowledge were found to have 73% adherence to the use of OAD. Based on the results of the correlation test with Chi-Square, obtained a significant value of p = 0.000. Conclusion iit can be stated that there is a relationship between the knowledge of type 2 DM patients and adherence to the use of OAD at the Singosari Public Health Center, Malang Regency.
Sodium-Glucose Co-transporter-2 Inhibitors in Heart Failure with Preserved Ejection Fraction: A Breakthrough in Improvement of Clinical Outcomes?
Authors: Berezin Alexander E. , Berezin Alexander A.
Published: May 2022

The conventional conception of the therapy of heart failure (HF) with reduced ejection fraction has been recently modified by adding sodium-glucose co-transporter-2 (SGLT2) inhibitors to the combination consisting of beta blockers, mineralocorticoid receptor antagonists, and angiotensin receptor-neprilysin inhibitors, with the aim of improving clinical outcomes. It remains unclear whether other sub-populations of patients with HF, having either HF with preserved ejection fraction (HFpEF) or HF with mildly reduced ejection fraction, are relevant candidates for the effective therapeutic intervention that includes SGLT2 inhibitors.

The purpose of the narrative review is to elucidate plausible perspectives for the clinical implementation of SGLT2 inhibitors into optimal medical therapy in patients with HFpEF. The authors searched the bibliographic databases (Embase, Medline, and the Web of Science) and the Cochrane Central to find English-written publications satisfying the purpose of this study. The authors included eight studies and two meta-analyses that have been reported as completed and found that there were high heterogeneous data regarding the fact that SGLT2 inhibitors had strict resemblance in their efficacy among patients with HFpEF with and without Type 2 diabetes. Due to the use of unpublished data and findings from the trials ended early, there is a lack of upper left ventricular ejection fraction threshold levels to identify inclusion criteria and no agreement in heart failure with reduced ejection fraction determination. However, the results of the meta-analysis, especially come from subgroups’ analysis, appeared to be relevantly optimistic for use of SGLT2 inhibitors in HFpEF therapy.
HUBUNGAN SELF-EFFICACY DENGAN KESIAPSIAGAAN MENGHADAPI BANJIR PADA MASYARAKAT DESA DUSUN DALAM KECAMATAN SIULAK KABUPATEN KERINCI
Authors: SIMANDALAHI TIURMAIDA
Published: May 2022

Tujuan penelitian adalah untuk mengetahui hubungan self-efficacy dengan kesiapsiagaan menghadapi banjir pada masyarakat Desa Dusun Dalam Kecamatan Siulak Kabupaten Kerinci. Metoda penelitian menggunakan deskriptif analitik dengan pendekatan cross sectional. Penelitian dilakukan di Desa Dusun Dalam Kecamatan Siulak Kabupaten Kerinci, selama 1 bulan dari Agustus - September 2019 dengan melibatkan semua kepala keluarga yang ada berjumlah 264 orang. Sampel berjumlah 69 orang, digunakan teknik Random Sampling. Analisa data secara univariat dan bivariat melalui uji Chi-Square, dan data diolah secara komputerasi. Hasil univariat didapatkan 59,4% masyarakat tidak siaga, 63,8% self-efficacy rendah. Uji bivariat menjelaskan ada hubungan self-efficacy dengan kesiapsiagaan menghadapi bencana banjir (p-value 0.026 < 0,05). Rekomendasi penelitian yang diusulkan adalah optimalisasi sosialisasi dan edukasi terkait banjir dan penanganannya secara terpadu, dengan melibatkan seluruh perangkat masyarakat, bekerjasama dengan tim kebencanaan daerah BPBD Kerinci,dan memberdayakan potensi kearifan lokal yang ada sehingga masyarakat memiliki kesiapsiagaan dalam menghadapi bencana banjir.

Kata kunci: self-efficacy; kesiapsiagaan; banjir
EVALUASI KESUKSESAN SISTEM PELATIHAN JARAK JAUH PADA BALAI DIKLAT KEUANGAN MAKASSAR DI MASA PANDEMI COVID-19
Authors: Iskandar Azwar
Published: May 2022

This study aims to measure the success of the implementation of distance learning systems in the Financial Education and Training Agency of Makassar during the pandemic. This study used data obtained from respondents through questionnaires and quantitative approaches through component or variant-based of Structural Equation Modelling (SEM) techniques with Partial Least Square (PLS). The results show that in general the distance learning system has not fully run well and successfully based on indicators or approach of DeLone and McLean model. The system quality affects user satisfaction and user satisfaction also affects the net benefits. Meanwhile, the information quality and service quality has no effect on user satisfaction. Financial Education and Training Agency of Makassar is expected to improve the quality of information and quality of services in order to improve the satisfaction of training participants that will have an impact on the achievement of learning outcomes.
RASIO KINERJA KEUANGAN DAERAH DAN PEMBIAYAAN INFRASTRUKTUR DI KABUPATEN BONDOWOSO
Authors: BUDITIAWAN KRISTIAN , Santoso Eko Budi
Published: May 2022

Pada era desentralisasi setiap daerah otonom di Indonesia memiliki kapasitas dan kemampuan untuk menetapkan arah tujuan pembangunan sesuai dengan kondisi daerah masing-masing termasuk juga dalam mengelola sumber-sumber keuangan daerah untuk mencapai tujuan pembangunan nasional di daerahnya. Desentralisasi fiskal dimaksudkan dapat menyeimbangkan laju pembangunan antar daerah sehingga tidak ada lagi dikotomi daerah maju dan tertinggal karena masing-masing daerah mempunyai kesempatan membiayai pembangunan di daerahnya secara mandiri sesuai potensi daerahnya. Undang-Undang Nomor 23 Tahun 2014 tentang Pemerintah Daerah membuka kesempatan yang luas kepada daerah untuk menjalankan roda pemerintahannya dengan memanfaatkan sumber daya yang tersedia di daerahnya dan sedikit bergantung kepada bantuan pemerintah pusat. Untuk itu Pemerintah Daerah berusaha menggunakan sumber-sumber pendapatan daerahnya dengan sebaik-baiknya untuk membiayai proses pemerintahan yang berlangsung. Analisis rasio kinerja keuangan adalah salah upaya untuk menilai kinerja keuangan daerah agar memenuhi unsur akuntabilitas tata kelola pemerintahan yang baik. Penelitian ini membahas tentang rasio kinerja keuangan daerah yang dan kemampuan daerah dalam mendanai penyediaan infrastruktur di Kabupaten Bondowoso. Metode analisis yang digunakan adalah analisis kinerja keuangan daerah yang meliputi aspek rasio efektifitas, rasio efisiensi, rasio kemandirian, rasio aktifitas, dan rasio pertumbuhan. Kinerja keuangan Kabupaten Bondowoso selama periode 2016-2020 dapat dikatakan secara keseluruhan sudah baik dari sisi erasio efektifita dan rasio efisiensi. Rasio kemandirian masih menunjukkan proporsi penerimaan transfer dari pemerintah Provinsi/pusat masih lebih besar dari pendapatan asli daerahnya. Rasio aktifitas menggambarkan bahwa penerimaan masih lebih banyak digunakan untuk membiayai belanja operasional dibandingkan dengan belanja modal (penyediaan infrastruktur). Pembiayaan infrastruktur dapat menggunakan dana yang bersumber dari Dana Alokasi Umum, Dana Alokasi Khusus, Pinjaman Daerah, kerjasama pemerintah dengan badan usaha, dan dana yang bersumber dari CSR perusahaan yang ada di Kabupaten Bondowoso.
PENGARUH MODAL PSIKOLOGIS DAN PENERIMAAN TEKNOLOGI TERHADAP SISTEM PEMERINTAHAN BERBASIS ELEKTRONIK PADA PEGAWAI NEGERI SIPIL PEMERINTAH KABUPATEN PAKPAK BHARAT
Authors: Nasution Mohd Farouq Hasymi
Published: May 2022

Kebijakan penerapan sistem pemerintahan berbasis elektronik dilakukan untuk mewujudkan tata Kelola pemerintahan yang bersih, efektif, transparana, dan akuntabel serta pelayanan publick yang berkualitas dan terpercaya. Namun dalam implementasinya oleh Pemerintah Kabupaten Pakpak Bharat belum maksimal karena masih terbiasanya dengan sistem birokrasi lama.    Penelitian ini bertujuan untuk memberikan gambaran kapasitas modal psikologis dan penerimaan teknologi dan menganalisis secara empiris pengaruh dari modal psikologis dan penerimaan teknologi terhadap sistem pemerintahan berbasis elektronik pada Pegawai Negeri Sipil Pemerintah Kabupaten Pakpak Bharat. Metode penelitian ini menggunakan penelitian kuantitaif dengan teknik analisis regresi linear berganda. Data penelitian dihasilkan melalui skala likert yang kemudian diolah dan dianlisis untuk menarik kesimpulan. Jumlah sampel yang digunakan dalam penelitian ini adalah 236 Pegawai Negeri Sipil Pemerintah Kabupaten Pakpak Bharat. Hasil penelitian ini menunjukkan secara umum Pegawai Negeri Sipil Pemerintah Kabupaten Pakpak Bharat memiliki kapasitas modal psikologis tinggi dan penerimaan teknologi yang sedang. Variabel Modal psikologis berpengaruh positif dan signifikan terhadap sikap penggunaan sistem pemerintahan berbasis elektronik. Variabel Penerimaan teknologi berpengaruh negatif dan signifikan terhadap sikap penggunaan sistem pemerintahan berbasis elektronik. Variabel modal psikologis dan penerimaan teknologi secara bersama-sama berpengaruh positif terhadap sikap penggunaan sistem pemerintahan berbasis elektronik dengan besar pengaruh sebesar 36.8 %. Pemkab Pakpak Bharat dapat meningkatkan upaya memfasilitasi pengembangan diri khususnya yang berkaitan dengan sistem pemerintahan berbasis elektronik kepada Pegawai Negeri Sipi Pemerintah kabupaten Pakpak Bharat dan menciptakan lingkungan kerja dengan teknologi yang dirasakan kemudahan dan kebermanfaatannya.      

 

Kata kunci: sistem pemerintahan berbasis elektronik; modal psikologis; penerimaan teknologi.
KEBIJAKAN AKUNTANSI LINGKUNGAN UNTUK MENDUKUNG PARIWISATA BERKELANJUTAN DI KAWASAN DANAU TOBA PROVINSI SUMATERA UTARA
Authors: Kholis Azizul
Published: May 2022

Penelitian ini bertujuan untuk menganalisis kebijakan akuntansi lingkungan yang dijalankan oleh perusahaan  dikawasan sekitar danau Toba provinsi Sumaera Utara. Berdasarkan teori pariwisata berkelanjutan mencakup tiga aspek yaitu ekonomi, lingkungan dan sosial maka ketiga faktor tersebut dalam penelitian ini diuji menjadi penentu kebijakan akuntansi lingkungan perusahaan. Proksi ekonomi diukur melalui dukungan manajemen perusahaan, proksi lingkungan adalah kebijakan CSR dan dan proksi sosial adalah peran masyarakat. Pengambilan sampel dilakukan pada 73 perusahaan di Kabupaten Simalungun, Toba, Samosir, Dairi, Humbang Hasundutan, Tapanuli Utara, dan Karo. Teknik analisis yang digunakan adalah regresi berganda dengan hasil penelitian menunjukkan bahwa Kebijakan CSR (X1), Dukungan Manajemen (X2) dan Dukungan Masyarakat (X3), memiliki pengaruh yang signifikan terhadap kebijakan akuntansi lingkungan perusahaan (Y). Keterbatasan penelitian ini adalah pada sampel yang tidak mengelompokkan perusahaan berdasarkan kategori bidang usaha, sehingga dapat saja berbeda kebijakan akuntansi lingkungan antara satu perusahaan dengan perusahan lainnya. Kebaruan dan inovasi penelitian ini adalah menghubungkan secara peta konsep kebijakan akuntansi lingkungan perusahaan yang didasarkan pada teori stakeholder dengan teori pariwisata berkelanjutan sehingga diharapkan secara kajian akademik kebijakan akuntansi lingkungan perusahaan memiliki kontribusi terhadap penguatan pariwisata berkelanjutan. Rekomendasi untuk penelitian selanjutnya adalah dapat memilih sampel penelitian pada satu kelompok kategori bidang usaha yang sejenis misalnya pada bisnis perhotelan, perusahaan agen perjalanan wisata ataupun jasa transportasi air. Hasil penelitian juga membuktikan bahwa kesadaran akan pariwisata berkelanjutan sudah dipahami oleh pihak manajemen perusahaan yang dibuktikan dengan adanya dukungan program CSR di kawasan Danau Toba.

 

Kata kunci: Akuntansi, Lingkungan, Pariwisata, Berkelanjutan
A cilostazol kortól és nemtől függetlenül javítja az életminőséget és az alsó végtagi funkcionális kapacitást alsó végtagi verőérbetegségben – a SHERIFF (SHort-tERm cIlostazol eFFicacy) vizsgálat új eredményei
Authors: Farkas Katalin , Kolossváry Endre , Járai Zoltán
Published: May 2022

A claudicatio intermittens jelentős negatív hatással van a betegek életminőségére. A különböző revascularisatiós eljárások és a noninvazív orvosi kezelések javíthatják a betegek járását. A cilostazol I.A ajánlással rendelkezik a claudicatio intermittens kezelésére. Vizsgálatunk célja a három hónapos cilostazolkezelésnek az egészséggel kapcsolatos életminőségre és az alsó végtag funkcionális kapacitására gyakorolt hatásának értékelése a klinikai gyakorlatban, claudicatio intermittensben szenvedő nőkben (F) és férfiakban (M), valamint 65 évnél fiatalabb (Y), illetve 65 éves vagy idősebb (O) betegek körében. A tanulmány egy multicentrikus, beavatkozással nem járó vizsgálat, amelybe 812, ambuláns kezelés alatt álló alsó végtagi verőérbetegségben (LEAD) szenvedő beteg (Fontaine II. stádium, átlagéletkor: 67,17 év, férfi/nő: 58,25/41,75%, életkor ≥65 év: n=506) került beválasztásra, akik cilostazolkezelést kaptak (50 vagy 100 mg naponta kétszer) három hónapig. Az életminőséget az EQ-5D-3L kérdőívvel, a funkcionális kapacitást a WELCH kérdőívvel értékeltük. A fájdalommentes és maximális járástávolság, valamint a boka-kar index (ABI) mérése történt meg a vizsgálat indulásakor és a három hónapos kezelés után. A vizsgálat befejezése után az EQ-5D index javult (kiindulási érték: F [nők] –0,49±0,23, M [férfiak] –0,44±0,22, Y [65 évnél fiatalabb] –0,45±0,21, O [≥65 év] –0,47±0,23; harmadik hónap: –0,27±0,18, –0,25±0,18, –0,25±0,18, –0,26±0,18; p <0,0001), és a WELCH-pontszám szintén szignifikánsan nőtt (kiindulási érték: F 18±13, M 20±14, Y 21±14, O 18±13; harmadik hónap: 31±18, 32±18, 32±19, 31±17; p <0,0001) mind a négy betegcsoportban. Mind a fájdalommentes, mind a maximális járástávolság nőtt: F 60,94%, (medián: +50,26%), 49,57% (medián: +42,86%), M 50,22% (medián: +50%), 37,7% (medián: +33,33%), Y 54,35% (medián: +56,2%), 36,78% (medián: +42,86%), O 54,62% (medián: +50%), 46,29% (medián: +33,33%) (p <0,001). Három hónapos cilostazolkezelés javította az életminőséget és az alsó végtagi funkcionális kapacitást claudicatio intermittensben szenvedő betegekben, kortól és nemtől függetlenül. A WELCH kérdőív hasznos eszköznek bizonyult a klinikai gyakorlatban a claudicatio kezelése során a járóképesség értékelésére.
Pakistan’s Lower Riparian Anxieties on the Indus and Indian Assurances
Authors: Mehsud Muhammad Imran , Jalal Iqra , Khan Tariq Anwar , Wazir Sajjad Ahmad
Published: May 2022

This paper analyses Pakistan’s lower riparian anxieties on the Indus and Indian assurances by elaborating the differing perspectives of the two states on different projects pursued by India on the western rivers; the rivers allotted to Pakistan by the Indus Waters Treaty of 1960. It argues that the water supply has reduced drastically in the last fifty years, which has resulted in a water crisis in India and Pakistan. India has started constructing different hydroelectric projects on western rivers in Jammu and Kashmir, which the treaty allows under certain conditionality. Pakistan objects to these projects as against the treaty. Pakistan considers the Indian projects a threat to its national integrity and the agriculture-based economy, as they could be used as a weapon to the detriment of downstream Pakistan. India assures Pakistan of no such intentions and defends the projects as in conformity with the Indus Water Treaty. This trust deficit between the two arch-rivals has made water disputes between them intractable.
Türkiye’de Düşünce Özgürlüğü Konusundaki Basılı Kaynakların Nicelik ve İçerik Analizi: 1928-2020
Authors: ANKARALI Ela , YILMAZ Bülent
Published: May 2022

Bilgi merkezlerinin işlevlerini yerine getirebilmesi için düşünce özgürlüğü kritik öneme sahiptir. Bilgi merkezleri sundukları kaynaklarla düşünce özgürlüğünü besler, düşünce özgürlüğü ise bilgi merkezlerinin her görüşe ait bilgi kaynağını hizmete sunmasına olanak sağlar. Türkiye’de düşünce özgürlüğü ve sansür üzerine yayımlanmış çok sayıda kaynak bulunmaktadır. Bu çalışma kapsamında, Türkiye’de 1928-2020 yılları arasında, düşünce özgürlüğü ve sansür üzerine yayımlanmış basılı kaynakların (kitap, makale ve tez) nicelik ve içerik analizi yapılmıştır. Çalışmada, “Türkiye’de düşünce özgürlüğü ve sansür konusunda yayımlanmış kaynakların türlerine göre dağılımı nasıl olmuştur, dönemlere göre sayısal dağılımı ve değişimi nasıl olmuştur ve dönemlere göre içerik değişimi nasıl olmuştur?” soruları yanıtlanmaya çalışılmıştır. Veri tabanlarından kapsamlı bir şekilde yapılan literatür taraması sonucunda ulaşılan kaynaklar kitap, makale ve tez olarak türlerine ayrılarak, kaynakların on yıllık dönemler için sayısal dağılım ve içerikleri değerlendirilmiştir. İçerik değerlendirmesi, başlıklarda kullanılan terimler üzerinden yapılmıştır. Çalışma kapsamında, 433’ü kitap, 638’i makale ve 232’si tez olmak üzere toplam 1303 kaynağa ulaşılmıştır. Çalışma sonucunda, düşünce özgürlüğü ve sansür ile ilgili yayın sayısının hızla yükselmekte olduğu, özellikle 2011-2020 döneminde yayımlanan kaynak sayısının önceki dönemlere göre büyük ölçüde arttığı ve başlıklarda kullanılan terimlerin değiştiği görülmüştür.
Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis
Authors: Birkholz Mario , Malti Danai Eleni , Hartmann Stephan , Neubauer Peter
Published: May 2022

Microalgae constitute an abundant source of poly-unsaturated fatty acids which are applied in various biotechnological fields such as pharmaceuticals and food supplement. Separating microalgae cells with respect to their lipid content would establish a relevant at-line analytical technique. The present study demonstrates an electrical approach for the separation of the lipid-producing microalgae Crypthecodinium cohnii using the effect of dielectrophoresis (DEP) in a microfluidic flow cell. Microalgae were cultivated for 8 days, while cell growth was characterized by optical density, dry cell weight, glucose concentration and lipid content via fluorescence microscopy. The size distribution of cells during cultivation was thoroughly investigated, since the DEP force scales with cell volume, but also depends on lipid content via cell electrophysiological constants. Thus, the challenge was to deconvolute one separation effect from the other, while the electrical cell constants of C. cohnii are not known yet. The DEP-dependent separation was realized by slanted top-bottom electrodes with the flowing cell suspension between them. Turning on the voltage deflected the cells from their initial path as determined by the streaming and thus changed their direction of flow. The separation efficiency of DEP was tested for various electrical field strengths and its performance was determined by quantitative analysis of optical and fluorescence videos. It could be shown for all size groups that the most lipid-containing cells were always subject to DEP separation and that the method is thus not only suitable for process analysis, but also for strain selection of the most productive cell lines.
A Novel DNA Repair Gene Signature for Immune Checkpoint Inhibitor-Based Therapy in Gastric Cancer
Authors: Yuan Binbin , Jiang Chengfei , Chen Lingyan , Wen Lihui , Cui Jinlong , Chen Min , Zhang Shu , Zhou Lin , Cai Yimeng , Mao Jian-Hua , Zou Xiaoping , Hang Bo , Wang Pin
Published: May 2022

Gastric cancer is a heterogeneous group of diseases with only a fraction of patients responding to immunotherapy. The relationships between tumor DNA damage response, patient immune system and immunotherapy have recently attracted attention. Accumulating evidence suggests that DNA repair landscape is a significant factor in driving response to immune checkpoint blockade (ICB) therapy. In this study, to explore new prognostic and predictive biomarkers for gastric cancer patients who are sensitive and responsive to immunotherapies, we developed a novel 15-DNA repair gene signature (DRGS) and its related scoring system and evaluated the efficiency of the DRGS in discriminating different molecular and immune characteristics and therapeutic outcomes of patients with gastric adenocarcinoma, using publicly available datasets. The results demonstrated that DRGS high score patients showed significantly better therapeutic outcomes for ICB compared to DRGS low score patients (p < 0.001). Integrated analysis of multi-omics data demonstrated that the patients with high DRGS score were characteristic of high levels of anti-tumor lymphocyte infiltration, tumor mutation burden (TMB) and PD-L1 expression, and these patients exhibited a longer overall survival, as compared to the low-score patients. Results obtained from HPA and IHC supported significant dysregulation of the genes in DRGS in gastric cancer tissues, and a positive correlation in protein expression between DRGS and PD-L1. Therefore, the DRGS scoring system may have implications in tailoring immunotherapy in gastric cancers. A preprint has previously been published (Yuan et al., 2021).
Characterization of the Interaction Between SARS-CoV-2 Membrane Protein (M) and Proliferating Cell Nuclear Antigen (PCNA) as a Potential Therapeutic Target
Authors: Zambalde Érika Pereira , Pavan Isadora Carolina Betim , Mancini Mariana Camargo Silva , Severino Matheus Brandemarte , Scudero Orlando Bonito , Morelli Ana Paula , Amorim Mariene Ribeiro , Bispo-dos-Santos Karina , Góis Mariana Marcela , Toledo-Teixeira Daniel A. , Parise Pierina Lorencini , Mauad Thais , Dolhnikoff Marisa , Saldiva Paulo Hilário Nascimento , Marques-Souza Henrique , Proenca-Modena José Luiz , Ventura Armando Morais , Simabuco Fernando Moreira
Published: May 2022

SARS-CoV-2 is an emerging virus from the Coronaviridae family and is responsible for the ongoing COVID-19 pandemic. In this work, we explored the previously reported SARS-CoV-2 structural membrane protein (M) interaction with human Proliferating Cell Nuclear Antigen (PCNA). The M protein is responsible for maintaining virion shape, and PCNA is a marker of DNA damage which is essential for DNA replication and repair. We validated the M-PCNA interaction through immunoprecipitation, immunofluorescence co-localization, and PLA (Proximity Ligation Assay). In cells infected with SARS-CoV-2 or transfected with M protein, using immunofluorescence and cell fractioning, we documented a reallocation of PCNA from the nucleus to the cytoplasm and the increase of PCNA and γH2AX (another DNA damage marker) expression. We also observed an increase in PCNA and γH2AX expression in the lung of a COVID-19 patient by immunohistochemistry. In addition, the inhibition of PCNA translocation by PCNA I1 and Verdinexor led to a reduction of plaque formation in an in vitro assay. We, therefore, propose that the transport of PCNA to the cytoplasm and its association with M could be a virus strategy to manipulate cell functions and may be considered a target for COVID-19 therapy.
Intraseasonal Drivers of the 2018 Drought Over São Paulo, Brazil
Authors: Gozzo Luiz Felippe , Drumond Anita , Pampuch Luana Albertani , Ambrizzi Tércio , Crespo Natália Machado , Reboita Michelle Simões , Bier Anderson Augusto , Carpenedo Camila Bertoletti , Bueno Paola Gimenes , Pinheiro Henri Rossi , Custodio Maria de Souza , Kuki Cassia Akemi Castro , Tomaziello Ana Carolina Nóbile , Gomes Helber Barros , da Rocha Rosmeri Porfírio , Coelho Caio A. S. , Pimentel Raíssa de Matos
Published: May 2022

Dry conditions occurred over São Paulo state (southeastern Brazil) from February to July 2018, causing the driest semester in 35 years. Socioeconomic impacts included a record number of fire spots, most adverse conditions to pollutant dispersion in 3 years and the winter's lowest water reservoirs stored volume in 17 years. This paper discusses climate drivers to the onset and persistence of the dry conditions, with special attention to the intraseasonal forcing. Barotropic atmospheric circulations forced by the intraseasonal Pacific-South America teleconnection pattern, embedded in the lower frequency setup of the Pacific Decadal Oscillation and the Atlantic Multidecadal Oscillation, were identified as main large-scale forcings to reduce precipitation. Drought evolution was modulated by other intraseasonal drivers such as the Madden Julian, Antarctic and 10–30 days Oscillations. A break in the 6-month dry condition, in March 2018, highlighted the important role of such oscillations in determining precipitation anomalies over SP. Results show that intraseasonal phenomena and their interactions control drought characteristics such as magnitude, persistence and spatial distribution within a setup determined by lower-frequency oscillations. The intraseasonal timescale seems to be key and must be considered for a complete description and understanding of the complex drought evolution process in São Paulo.
Assessing Peroxygenase-Mediated Oxidations in the Presence of High Concentrations of Water-Miscible Co-Solvents
Authors: Hilberath Thomas , van Troost Anouska , Alcalde Miguel , Hollmann Frank
Published: May 2022

The use of water-miscible organic co-solvents in biocatalysis is a simple procedure for obtaining higher enzymatic activities toward hydrophobic substrates. However, effects on activity and stability have to be carefully evaluated, also with regard to the type and concentration of the respective co-solvent. In this contribution, we investigated and evaluated the effect of some common water-miscible co-solvents on the biocatalytic performance of the recombinant unspecific peroxygenase rAaeUPO from Agrocybe aegerita. rAaeUPO showed promising activities in the presence of high concentrations of the best co-solvent acetonitrile, which enabled to use higher substrate concentrations (≥100 mM). Employing high acetonitrile concentrations for UPO-mediated oxidation of ethylbenzene to (R)-1-phenylethanol was demonstrated under preparative scale conditions and led to product accumulation rates of 31 mM h−1.
Cardiac Adaptation to Prolonged High Altitude Migration Assessed by Speckle Tracking Echocardiography
Authors: Chen Xu , Liu Bohan , Deng Yujiao , Yang Feifei , Wang Wenjun , Lin Xixiang , Yu Liheng , Pu Haitao , Zhang Peifang , Li Zongren , Zhong Qin , Jia Qian , Li Yao , Wang Xiao , Chen Wei , Burkhoff Daniel , He Kunlun
Published: May 2022

Objective

Exposure to high altitudes represents physiological stress that leads to significant changes in cardiovascular properties. However, long-term cardiovascular adaptions to high altitude migration of lowlanders have not been described. Accordingly, we measured changes in cardiovascular properties following prolonged hypoxic exposure in acclimatized Han migrants and Tibetans.

Methods

Echocardiographic features of recently adapted Han migrant (3–12 months,n= 64) and highly adapted Han migrant (5–10 years,n= 71) residence in Tibet (4,300 m) using speckle tracking echocardiography were compared to those of age-matched native Tibetans (n= 75) and Han lowlanders living at 1,400 m (n= 60).

Results

Short-term acclimatized migrants showed increased estimated pulmonary artery systolic pressure (PASP) (32.6 ± 5.1 mmHg vs. 21.1 ± 4.2 mmHg,p< 0.05), enlarged right ventricles (RVs), and decreased fractional area change (FAC) with decreased RV longitudinal strain (−20 ± 2.8% vs. −25.5 ± 3.9%,p< 0.05). While left ventricular ejection fraction (LVEF) was preserved, LV diameter (41.7 ± 3.1 mm vs. 49.7 ± 4.8 mm,p< 0.05) and LV longitudinal strain (−18.8 ± 3.2% vs. −22.9 ± 3.3%,p< 0.05) decreased. Compared with recent migrants, longer-term migrants had recovered RV structure and functions with slightly improved RV and LV longitudinal strain, though still lower than lowlander controls; LV size remained small with increased mass index (68.3 ± 12.7 vs. 59.3 ± 9.6,p< 0.05). In contrast, native Tibetans had slightly increased PASP (26.1 ± 3.4 mmHg vs. 21.1 ± 4.2 mmHg,p< 0.05) with minimally altered cardiac deformation compared to lowlanders.

Conclusion

Right ventricular systolic function is impaired in recent (<1 year) migrants to high altitudes but improved during the long-term dwelling. LV remodeling persists in long-term migrants (>5 years) but without impairment of LV systolic or diastolic function. In contrast, cardiac size, structure, and function of native Tibetans are more similar to those of lowland dwelling Hans.
Modification of Cardiovascular Drugs in Advanced Heart Failure: A Narrative Review
Authors: Martínez-Sellés Manuel , Grodzicki Tomasz
Published: May 2022

Advanced heart failure (HF) is a complex entity with a clinical course difficult to predict. However, most patients have a poor prognosis. This document addresses the modification of cardiovascular drugs in patients with advanced HF that are not candidates to heart transplantation or ventricular assist device and are in need of palliative care. The adjustment of cardiovascular drugs is frequently needed in these patients. The shift in emphasis from life-prolonging to symptomatic treatments should be a progressive one. We establish a series of recommendations with the aim of adjusting drugs in these patients, in order to adapt treatment to the needs and wishes of each patient. This is frequently a difficult process for patients and professionals, as drug discontinuing needs to balance treatment benefit with the psychological adaption to having a terminal illness. We encourage the use of validated assessment tools to assess prognosis and to use this information to take clinical decisions regarding drug withdrawal and therapeutic changes. The golden rule is to stop drugs that are harmful or non-essential and to continue the ones that provide symptomatic improvement.
Regulation of Endothelial Progenitor Cell Functions in Ischemic Heart Disease: New Therapeutic Targets for Cardiac Remodeling and Repair
Authors: Huang Huai , Huang Weiqiang
Published: May 2022

Ischemic heart disease (IHD) is the leading cause of morbidity and mortality worldwide. Ischemia and hypoxia following myocardial infarction (MI) cause subsequent cardiomyocyte (CM) loss, cardiac remodeling, and heart failure. Endothelial progenitor cells (EPCs) are involved in vasculogenesis, angiogenesis and paracrine effects and thus have important clinical value in alternative processes for repairing damaged hearts. In fact, this study showed that the endogenous repair of EPCs may not be limited to a single cell type. EPC interactions with cardiac cell populations and mesenchymal stem cells (MSCs) in ischemic heart disease can attenuate cardiac inflammation and oxidative stress in a microenvironment, regulate cell survival and apoptosis, nourish CMs, enhance mature neovascularization, alleviate adverse ventricular remodeling after infarction and enhance ventricular function. In this review, we introduce the definition and discuss the origin and biological characteristics of EPCs and summarize the mechanisms of EPC recruitment in ischemic heart disease. We focus on the crosstalk between EPCs and endothelial cells (ECs), smooth muscle cells (SMCs), CMs, cardiac fibroblasts (CFs), cardiac progenitor cells (CPCs), and MSCs during cardiac remodeling and repair. Finally, we discuss the translation of EPC therapy to the clinic and treatment strategies.
Cellular Crosstalk in the Vascular Wall Microenvironment: The Role of Exosomes in Vascular Calcification
Authors: Wu Yun-Yun , Shan Su-Kang , Lin Xiao , Xu Feng , Zhong Jia-Yu , Wu Feng , Duan Jia-Yue , Guo Bei , Li Fu-Xing-Zi , Wang Yi , Zheng Ming-Hui , Xu Qiu-Shuang , Lei Li-Min , Ou-Yang Wen-Lu , Tang Ke-Xin , Li Chang-Chun , Ullah Muhammad Hasnain Ehsan , Yuan Ling-Qing
Published: May 2022

Vascular calcification is prevalent in aging, diabetes, chronic kidney disease, cardiovascular disease, and certain genetic disorders. However, the pathogenesis of vascular calcification is not well-understood. It has been progressively recognized that vascular calcification depends on the bidirectional interactions between vascular cells and their microenvironment. Exosomes are an essential bridge to mediate crosstalk between cells and organisms, and thus they have attracted increased research attention in recent years. Accumulating evidence has indicated that exosomes play an important role in cardiovascular disease, especially in vascular calcification. In this review, we introduce vascular biology and focus on the crosstalk between the different vessel layers and how their interplay controls the process of vascular calcification.
Study on Organic Matter Enrichment and Reservoir Characteristics of Permian Longtan Formation Shale in Southeast Sichuan
Authors: Cao Qian , Qi Minghui , Li Xiawei , Wang Daifu , Deng Yuwei , Wu Chongyu
Published: May 2022

The study of reservoir space development characteristics and the geochemical parameters of the Permian Longtan Formation in the southeast of the Sichuan Basin at Well X1 was analyzed by using core observations, optical thin-section observations, Ar-ion polishing, scanning electron microscopy, and nitrogen adsorption tests. Major and trace elements of samples, the V/Cr, U/T, enrichment coefficients EF (Mo) and EF (U), chemical alteration indexes CIA and Sr/Cu, and Mo, P, and other index values of different samples were calculated based on the test of organic geochemical parameters. The depositional environment of the Longtan Formation in the study area was systematically analyzed. The Longtan Formation was in a warm and humid climate during the deposition period. The main body of the sedimentary water was in an oxygen-depleted state, and a portion of it was in an oxidized state with high biological productivity. Therefore, it had a small deposition rate. The clay mineral content and organic matter content in the sample played a major role in controlling the development of reservoir space. The content of clay minerals in the sample was high, and it was mainly chlorite and a mixed layer of illite/smectite. Under a microscope, it was observed that the mixed layer of flake illite/smectite and chlorite aggregate mostly developed interlayer pores or microfractures, which played a positive role in the specific surface area and pore volume of the sample.
The Philippine Teachers Concerns on Educational Reform Using Concern Based Adoption Model
Authors: Magallanes Krizia , Chung Jae Young , Lee Sunbok
Published: May 2022

This study aims to identify the Philippines teachers’ concerns in K–12 implementation. The Concerns Based Adoption Model was applied to determine the level of concerns, and the Stages of Concern Questionnaire has been administered to 400 teachers. Findings indicate that consequence and collaboration was the teachers’ current concern (impact stage). Furthermore, experience and education factors showed the biggest significance affecting their collaboration among teachers. These current concerns match the existing problems of Philippine education: poor PISA results and lack of resources. This research urges the Philippine government to promote professional development activities that encourage teamwork and collaboration among teachers.
Fast and Accurate Health Assessment of Lithium-Ion Batteries Based on Typical Voltage Segments
Authors: Yang Ning , Yu Tao , Luo Qingquan , Wang Keying
Published: May 2022

Lithium-ion batteries are widely employed in industries and daily life. Research on the state of health (SOH) of batteries is essential for grasping the performance of batteries, better guiding battery health management, and avoiding safety mishaps caused by battery aging. Nowadays, most research adopts a data-driven artificial intelligence approach to assess SOH. However, the majority of approaches are based on entire voltage, current, or temperature curves. In reality, voltage, current, and temperature are frequently presented in segments, leading to the limited flexibility and slow analysis speed of the traditional techniques. This study solves the problem by dividing the whole voltage curve into many typical kinds of segments with equal timescales based on different typical voltage beginning points. On this foundation, the temporal convolution network (TCN) is used to create a sub-model of SOH estimation for several typical kinds of segments. In addition, the sub-models are fused using the bootstrap aggregating (Bagging) approach to boost accuracy. Finally, this research uses a publicly available dataset from Oxford to demonstrate the effectiveness of the suggested strategy.
An Overview on the Taxonomy, Phylogenetics and Ecology of the Psychedelic Genera Psilocybe, Panaeolus, Pluteus and Gymnopilus
Authors: Strauss Dominique , Ghosh Soumya , Murray Zurika , Gryzenhout Marieka
Published: May 2022

Psilocybin and psilocin, two psychoactive components found in “magic mushrooms,” have therapeutic potential in a number of mental health disorders without the addictiveness and overdose risks found in other mind-altering drugs, such as cocaine, methamphetamines and alcohol. Psychedelic mushrooms occur naturally, are wide distributed and easily accessible. The need for reviews and comprehensive field guides is urgent due to the recent surge of research into psychedelic mushrooms along with public interest. Psilocybin and psilocin are recorded in mushroom species of Psilocybe, Panaeolus, Pluteus, and Gymnopilus. This review discusses species identification, taxonomy and classification, available DNA sequence data and psychedelic species in Psilocybe, Panaeolus, Pluteus, and Gymnopilus, as well as similar looking genera that could be harmful.
A Connexin Gene (GJB3) Mutation in a Chinese Family With Erythrokeratodermia Variabilis, Ichthyosis and Nonsyndromic Hearing Loss: Case Report and Mutations Update
Authors: Gao Yajuan , Zhang Qianli , Zhang Shiyu , Yang Lu , Liu Yaping , Liu Yuehua , Wang Tao
Published: May 2022

Background: Gap junctions formed by connexins are channels on cytoplasm functioning in ion recycling and homeostasis. Some members of connexin family including connexin 31 are significant components in human skin and cochlea. In clinic, mutations of connexin 31 have been revealed as the cause of a rare hereditary skin disease called erythrokeratodermia variabilis (EKV) and non-syndromic hearing loss (NSHL).

Objective: To determine the underlying genetic cause of EKV, ichthyosis and NSHL in three members of a Chinese pedigree and skin histologic characteristics of the EKV patient.

Methods: By performing whole exome sequencing (WES), Sanger sequencing and skin biopsy, we demonstrate a Chinese pedigree carrying a mutation of GJB3 with three patients separately diagnosed with EKV, ichthyosis and NSHL.

Results: The proband, a 6-year-old Chinese girl, presented with demarcated annular red-brown plaques and hyperkeratotic scaly patches on her trunk and limbs. Her mother has ichthyosis with hyperkeratosis and geographic tongue while her younger brother had NSHL since birth. Mutation analysis revealed all of them carried a heterozygous missense mutation c.293G>A of GJB3. Skin biopsy showed many grain cells with dyskeratosis in the granular layer. Acanthosis, papillomatosis, and a mild superficial perivascular lymphocytic infiltrate were observed.

Conclusion: A mutation of GJB3 associated with EKV, ichthyosis and NSHL is reported in this case. The daughter with EKV and the son with NSHL in this Chinese family inherited the mutation from their mother with ichthyosis. The variation of clinical features may involve with genetic, epigenetic and environmental factors.
Chronic Stress and Ovulatory Dysfunction: Implications in Times of COVID-19
Authors: Vigil Pilar , Meléndez Jaime , Soto Hugo , Petkovic Grace , Bernal Yanara A. , Molina Santiago
Published: May 2022

Stress is known to be associated with adverse health outcomes. The COVID-19 pandemic and its associated lockdowns are examples of chronic stressors. Lockdown measures inadvertently caused significant psychological distress and became a powerful source of anxiety/stress, sleep disturbances, nutritional changes and weight gain. Stress is known to impact women's health specifically, through hypothalamic-pituitary-gonadal (HPG) axis dysfunction and resultant ovulatory dysfunction. Such dysfunction may manifest in menstrual irregularities and/or infertility due to hypothalamic hypogonadism. Here, we review the key physiological mediators of stress and associated ovulatory dysfunction. The kisspeptinergic system is comprised of sets of neurons located in the hypothalamus, the rostral periventricular region of the third ventricle (RP3V) and the arcuate nucleus (ARC). This system links nutrition, reproductive signals and stress. It plays a key role in the function of the HPG axis. During chronic stress, the kisspeptinergic system affects the HPG axis, GnRH pulsatility, and, therefore, ovulation. Leptin, insulin and corticotrophin-releasing hormone (CRH) are thought to be additional key modulators in the behavioral responses to chronic stress and may contribute to stress-related ovulatory dysfunction. This mini-review also summarizes and appraises the available evidence on the negative impact of chronic stress as a result of the COVID-19 pandemic lockdowns. It proposes physiological mechanisms to explain the observed effects on women's reproductive health and well-being. The review suggests areas for future research.