bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Intracellular Ca2+ and Mitochondrial ROS in Small Aβ1-42 Oligomer-Induced Microglial Death
Authors: Jekabsone Aiste , Jankeviciute Silvija , Pampuscenko Katryna , Borutaite Vilmante , Morkuniene Ramune
Published: August 2023

Alzheimer’s disease (AD) is the most common form of dementia worldwide, and it contributes up to 70% of cases. AD pathology involves abnormal amyloid beta (Aβ) accumulation, and the link between the Aβ1-42 structure and toxicity is of major interest. NMDA receptors (NMDAR) are thought to be essential in Aβ-affected neurons, but the role of this receptor in glial impairment is still unclear. In addition, there is insufficient knowledge about the role of Aβ species regarding mitochondrial redox states in neurons and glial cells, which may be critical in developing Aβ-caused neurotoxicity. In this study, we investigated whether different Aβ1-42 species—small oligomers, large oligomers, insoluble fibrils, and monomers—were capable of producing neurotoxic effects via microglial NMDAR activation and changes in mitochondrial redox states in primary rat brain cell cultures. Small Aβ1-42 oligomers induced a concentration- and time-dependent increase in intracellular Ca2+ and necrotic microglial death. These changes were partially prevented by the NMDAR inhibitors MK801, memantine, and D-2-amino-5-phosphopentanoic acid (DAP5). Neither microglial intracellular Ca2+ nor viability was significantly affected by larger Aβ1-42 species or monomers. In addition, the small Aβ1-42 oligomers caused mitochondrial reactive oxygen species (mtROS)-mediated mitochondrial depolarization, glutamate release, and neuronal cell death. In microglia, the Aβ1-42-induced mtROS overproduction was mediated by intracellular calcium ions and Aβ-binding alcohol dehydrogenase (ABAD). The data suggest that the pharmacological targeting of microglial NMDAR and mtROS may be a promising strategy for AD therapy.
CRISPR/Cas9: A Powerful Strategy to Improve CAR-T Cell Persistence
Authors: Wei Wei , Chen Zhi-Nan , Wang Ke
Published: August 2023

As an emerging treatment strategy for malignant tumors, chimeric antigen receptor T (CAR-T) cell therapy has been widely used in clinical practice, and its efficacy has been markedly improved in the past decade. However, the clinical effect of CAR-T therapy is not so satisfying, especially in solid tumors. Even in hematologic malignancies, a proportion of patients eventually relapse after receiving CAR-T cell infusions, owing to the poor expansion and persistence of CAR-T cells. Recently, CRISPR/Cas9 technology has provided an effective approach to promoting the proliferation and persistence of CAR-T cells in the body. This technology has been utilized in CAR-T cells to generate a memory phenotype, reduce exhaustion, and screen new targets to improve the anti-tumor potential. In this review, we aim to describe the major causes limiting the persistence of CAR-T cells in patients and discuss the application of CRISPR/Cas9 in promoting CAR-T cell persistence and its anti-tumor function. Finally, we investigate clinical trials for CRISPR/Cas9-engineered CAR-T cells for the treatment of cancer.
Functional Evidence of CCDC186 as a New Disease-Associated Gene with Endocrine and Central Nervous System Alterations
Authors: Arrabal Luisa , Muñoz-Pujol Gerard , Medina Martínez Inmaculada , Gort Laura , García-Villoria Judit , Roldán Susana , Tort Frederic , Ribes Antonia
Published: August 2023

CCDC186 protein is involved in the maturation of dense-core vesicles (DCVs) in the trans-Golgi network in neurons and endocrine cells. Mutations in genes involved in DCV regulation, other than CCDC186, have been described in patients with neurodevelopmental disorders. To date, only one patient, within a large sequencing study of 1000 cases, and a single case report with variants in CCDC186, had previously been described. However, no functional studies in any of these two cases had been performed. We identified three patients from two gypsy families, unrelated to each other, with mutations in the CCDC186 gene. Clinically, all patients presented with seizures, frontotemporal atrophy, hypomyelination, recurrent infections, and endocrine disturbances such as severe non-ketotic hypoglycemia. Low levels of cortisol, insulin, or growth hormone could only be verified in one patient. All of them had a neonatal onset and died between 7 months and 4 years of age. Whole exome sequencing identified a homozygous variant in the CCDC186 gene (c.2215C>T, p.Arg739Ter) in the index patients of both families. Protein expression studies demonstrated that CCDC186 was almost undetectable in fibroblasts and muscle tissue. These observations correlated with the transcriptomic analysis performed in fibroblasts in one of the patients, which showed a significant reduction of CCDC186 mRNA levels. Our study provides functional evidence that mutations in this gene have a pathogenic effect on the protein and reinforces CCDC186 as a new disease-associated gene. In addition, mutations in CCDC186 could explain the combined endocrine and neurologic alterations detected in our patients.
Genome-Wide Analysis of Haemonchus contortus Proteases and Protease Inhibitors Using Advanced Informatics Provides Insights into Parasite Biology and Host–Parasite Interactions
Authors: Zheng Yuanting , Young Neil D. , Song Jiangning , Gasser Robin B.
Published: August 2023

Biodiversity within the animal kingdom is associated with extensive molecular diversity. The expansion of genomic, transcriptomic and proteomic data sets for invertebrate groups and species with unique biological traits necessitates reliable in silico tools for the accurate identification and annotation of molecules and molecular groups. However, conventional tools are inadequate for lesser-known organismal groups, such as eukaryotic pathogens (parasites), so that improved approaches are urgently needed. Here, we established a combined sequence- and structure-based workflow system to harness well-curated publicly available data sets and resources to identify, classify and annotate proteases and protease inhibitors of a highly pathogenic parasitic roundworm (nematode) of global relevance, called Haemonchus contortus (barber’s pole worm). This workflow performed markedly better than conventional, sequence-based classification and annotation alone and allowed the first genome-wide characterisation of protease and protease inhibitor genes and gene products in this worm. In total, we identified 790 genes encoding 860 proteases and protease inhibitors representing 83 gene families. The proteins inferred included 280 metallo-, 145 cysteine, 142 serine, 121 aspartic and 81 “mixed” proteases as well as 91 protease inhibitors, all of which had marked physicochemical diversity and inferred involvements in >400 biological processes or pathways. A detailed investigation revealed a remarkable expansion of some protease or inhibitor gene families, which are likely linked to parasitism (e.g., host–parasite interactions, immunomodulation and blood-feeding) and exhibit stage- or sex-specific transcription profiles. This investigation provides a solid foundation for detailed explorations of the structures and functions of proteases and protease inhibitors of H. contortus and related nematodes, and it could assist in the discovery of new drug or vaccine targets against infections or diseases.
The Impact of Facial Burns on Short- and Long-Term Quality of Life and Psychological Distress—A Prospective Matched Cohort Study
Authors: Palackic Alen , Franco-Mesa Camila , Beck Inessa , Nolte Steffen , Tapking Christian , Panayi Adriana , Stolle Annette , Haug Valentin , Hirche Christoph , Kneser Ulrich , Hundeshagen Gabriel
Published: August 2023

Burn injuries are a major healthcare challenge worldwide, with up to 50% of all minor burns located on the head and neck. With this study, we sought to describe the effect of facial burns (FB) on health-related quality of life through a prospective and matched cohort study design. Patients completed the 36 Item Short Form (SF-36) and the Hospital Anxiety and Depression Scale (HADS). Results were analyzed based on the distribution of datasets. In total, 55 patients with FB and 55 age-and sex-matched candidates were recruited. The most common mechanism of thermal injury was burns from flames. The FB group scored lower in physical and psychological dimensions than the control group, both acutely and one year after injury. An analysis of each domain showed that subjects in the FB group trended toward improvements in their score after one-year post-burn in physical functioning (acute: 71.0 ± 29.2; one-year: 83.7 ± 23.9; p = 0.02) and bodily pain (acute: 58.5 ± 30.3; one-year: 77.9 ± 30.5; p = 0.01) domains. Additionally, the FB group had significanlyt higher scores for anxiety (FB: 4.8 ± 3.2; control: 2.5 ± 2.8; p = <0.002) and depression (FB: 3.9 ± 3.5; control: 2.1 ± 2.7; p = 0.01) compared to the control. In conclusion, facial burns are associated with physical and psychosocial deficits, as well as elevated levels of psychological distress.
How to Improve Clinical Outcomes in Patients with Tachycardia-Induced Cardiomyopathy. Comment on Katz et al. Long-Term Outcomes of Tachycardia-Induced Cardiomyopathy Compared with Idiopathic Dilated Cardiomyopathy. J. Clin. Med. 2023, 12, 1412
Authors: Kataoka Naoya , Imamura Teruhiko
Published: August 2023

The clinical course of tachycardia-induced cardiomyopathy (TICM) has not yet been well studied thus far [...]
Snaring Self-Expanding Devices to Facilitate Transcatheter Aortic Valve Replacement in Patients with Complex Aortic Anatomies
Authors: Yao Yi-Jun , Zhao Zhen-Gang , Wang Xi , Peng Yong , Wei Jia-Fu , He Sen , Li Yi-Jian , He Jing-Jing , Zhu Zhong-Kai , Feng Yuan , Chen Mao
Published: August 2023

The snare-assisted technique has been described to facilitate transcatheter aortic valve replacement (TAVR) delivery system advancement in complex aortic anatomies. However, the evidence is limited to case reports. To evaluate the safety profile of the snare-facilitated approach and its impact on self-expanding (SE) TAVR outcomes, we collected consecutive patients who underwent transfemoral SE-TAVR for aortic stenosis, using propensity score matching (PSM) separately in tricuspid and type-0 and type-1 bicuspid aortic valve morphology between the snare and non-snare groups. In 766 patients, despite the snare group having significantly larger annulus angulation and maximal ascending aortic diameter, both groups achieved comparable 30-day device success rates, regardless of first-generation or new-generation valve use. After PSM, the snare group had a significantly lower new permanent pacemaker implantation rate among 193 type-0 patients (3.3% vs. 18.3%, p = 0.01). The ipsilateral group used new-generation valves less frequently (23.0% vs. 75.4%, p < 0.001), but there were no significant inter-group differences in procedure-related events, except for a lower incidence of PVL ≥ mild in the ipsilateral group (14.9% vs. 32.3%, p = 0.01). In conclusion, the snare-assisted technique appears useful in SE-TAVR with angulated aortic root anatomy, and the benefits were comparable between ipsilateral and contralateral snare techniques.
Activating Transcription Factor 1 (ATF1) Immunohistochemical Marker Distinguishes HCCC from MEC
Authors: Badawy Wafaey , Abdelfattah Asmaa S. , Sallam Haneen A.
Published: August 2023

The study aimed to compare 15 cases of mucoepidermoid carcinoma (MEC) and 15 cases of hyalinizing clear cell carcinoma (HCCC) using immunohistochemical staining and molecular analysis. Thirty samples were examined, and markers, including p63, CK5/6, SOX10, CK7, ATF1, and FISH probes specific to EWSR1 and MAML2, were used. Clear cell differentiation was observed in all MEC cases to some extent, with clear cell MEC showing the most prominent findings. Clear cell features were also present in conventional MEC, oncocytic MEC, and Warthin-like MEC, although to a lesser extent. The majority of cases were classified as low-grade MECs. MAML2 rearrangement was detected in all cases (except cases 11 and 14), while EWSR1 rearrangement was observed in a single case of clear cell MEC. These findings helped identify distinct subtypes within the mucoepidermoid carcinoma spectrum. The study emphasized the importance of utilizing immunohistochemical profiles, histopathological features, and molecular analysis for accurate diagnosis and classification of salivary gland neoplasms. HCCC was also discussed, and ATF1 was proposed as a marker to distinguish HCCC from morphologically similar neoplasms. The study concluded that a comprehensive approach combining immunohistochemistry, histopathology, and clinical correlation is essential for accurate diagnosis and classification, considering the variable expression of markers and potential overlap with other tumor types.
On the Measurement of Ocean Near-Surface Current from a Moving Buoy
Authors: Herrera-Vázquez Carlos F. , Rascle Nicolas , Ocampo-Torres Francisco J. , Osuna Pedro , García-Nava Héctor
Published: August 2023

This paper studies the error that occurs when measuring surface currents with a current meter mounted on a buoy or a mooring line whose horizontal and vertical motions respond to the presence of waves. The error is defined with respect to an Eulerian reference measurement where the sensor does not move. First, we present the subject with a theoretical analysis in the case of a monochromatic wave. That idealized model allows us to study particular sensor or mooring line motions. Second, a realistic numerical model is implemented to reconstruct the current field with a high resolution near the surface. Wave orbital velocities are generated with a random phase model. An Ekman-type current, uniform in the horizontal but with a vertical shear, is also incorporated. The results indicate that the error in the current measurement is highly dependent on the sensor motion induced by waves. The error magnitude is proportional to the wave momentum or Stokes drift and depends on the wave development state and the wind-generated current’s magnitude. The error obtained in the current measurement is analyzed by considering that the buoy only responds to low-frequency waves up to a maximum frequency. That maximum frequency is referenced concerning the peak frequency of the third moment of the spectrum (i.e., the Stokes drift spectrum). It allows us to classify the current time average into three ranges with respect to the maximum frequency: (1) Eulerian average, (2) wave-following average, and (3) intermediate case of undulating average where results cannot be generalized. The measurement error is most important in the region above the wave troughs. However, the error is also considerable in the region confined below the wave troughs and down to the Stokes drift e-folding depth. The error is particularly relevant in conditions of developed and energetic waves (Hs>3 m), where the surface Stokes drift can reach values above 0.1 m/s. It should be noted that measurement error can exceed the value of the Stokes drift at the sensor depth for certain mooring line motions. Those results should help better interpret in situ near-surface current measurements obtained from various devices.
Design, Analysis and Maintenance of Green, Innovative Marine Structures
Authors: Parunov Joško , Garbatov Yordan
Published: August 2023

This Special Issue includes eleven research studies on structural design, analysis and maintenance of green, innovative marine structures [...]
Critical Node Identification of Multi-UUV Formation Based on Network Structure Entropy
Authors: Chen Yi , Liu Lu , Zhang Xiaomeng , Qiao Wei , Ren Ranzhen , Zhu Boyu , Zhang Lichuan , Pan Guang , Yu Yang
Published: August 2023

In order to identify and attack the multi-UUV (unmanned underwater vehicle) groups, this paper proposes a method for identifying the critical nodes of multi-UUV formations. This method helps in combating multi-UUV formations by identifying the key nodes to attack them. Moreover, these multi-UUV formations are considered to have an unknown structure as the research object. Therefore, the network structure of the formation is reconstructed according to its space–time trajectory, and the importance of nodes is determined based on network structure entropy. As for the methodology, firstly, based on the swarm intelligence behavior method, the motion similarity of multi-UUV nodes in the formation is analyzed in pairs; furthermore, the leader–follower relationship and the network structure of the formation are calculated successively. Then, based on this network structure, the importance of the network nodes is further determined by the network structure entropy method. Finally, through simulation and experiments, it is verified that the algorithm can accurately construct the network structure of the unknown multi-UUV formation, and the accuracy of the calculated time delay data reaches 84.6%, and compared with the traditional information entropy algorithm, the ordering of the important nodes obtained by this algorithm is more in line with the underwater formation network.
An Overview of Nanotherapeutic Drug Delivery Options for the Management of Glioblastoma
Authors: Pentz William H. , Pizzuti Vincenzo J. , Halbert Matthew E. , Plute Tritan J. , Lockman Paul R. , Sprowls Samuel A.
Published: August 2023

Glioblastoma is the most common primary, malignant brain tumor that remains uniformly lethal in nearly all cases as a result of extreme cellular heterogeneity, treatment resistance, and recurrence. A major hurdle in therapeutic delivery to brain tumors is the blood–brain barrier (BBB), which is the tightly regulated vascular barrier between the brain parenchyma and systemic circulation that prevents distribution of otherwise beneficial chemotherapeutics to central nervous system tumors. To overcome the obstacle of drug delivery beyond the BBB, nanoparticle formulations have come to the forefront, having demonstrated success in preclinical observations, but have not translated well into the clinical setting. In summary, this review article discusses brain tumors and challenges for drug delivery caused by the BBB, explores the benefits of nanoparticle formulations for brain tumor delivery, describes the characteristics these formulations possess that make them attractive therapeutic strategies, and provides preclinical examples that implement nanoparticles within glioma treatment regimens. Additionally, we explore the pitfalls associated with clinical translation and conclude with remarks geared toward overcoming these issues.
Magnetic Resonance Imaging with Active Implantable Hearing Devices: Reports from the Daily Radiological Routine in an Outpatient MR Center
Authors: Fruehwald-Pallamar Julia , Fruehwald Franz , Holzer-Fruehwald Laura , Nolz Richard , Stoiber Christian , Sprinzl Georg Mathias
Published: August 2023

Purpose: For people with hearing implants (HI), magnetic resonance imaging (MRI) still presents some difficulties due to the built-in magnet. Radiologists often have concerns regarding complications associated with HIs. The aim of this study was to record the experiences of HI users during and after MRI examinations. Method: A survey including 15 questions regarding MRI specifics, namely changes in hearing ability, hearing/sound impressions, pain, uncomfortable feelings, etc., were mailed to our patients. Results: Overall, 79 patients with HI had a total of 159 MR examinations in our institute. A total of 45 HI recipients reported back: 35% stated that they had been rejected by an MRI Institute because of their HI. Their feelings/impression ratings during the measurements were not present and therefore were not rated for the majority (49%), 42% of the HI users rated the pain with 0 (no pain), 2% with 1 (very light pain), 4% with 5 (acceptable pain), and 2% rated the pain with 7, which is between acceptable and strong pain. One examination resulted in a dislocation of the magnet of a cochlear implant (CI 512 Cochlear Limited). No adverse events were reported for MED-EL HI users in the survey (none of the contacted AB users answered the questionnaire). The reported mean daily wearing time was 11.6 ± 4.6 h per day for 6.3 ± 1.7 days per week. Conclusions: Based on these results and our experience we conclude that MRI examinations with HI are safe given that the measurements are performed according to the safety policies and procedures released by the manufacturers.
Risk Factors and Clinical Outcomes after Antegrade Intramedullary Nailing in Proximal Humeral Fractures: Insights and Implications for Patient Satisfaction
Authors: Willauschus Maximilian , Grimme Sebastian , Loose Kim , Rüther Johannes , Millrose Michael , Biber Roland , Gesslein Markus , Bail Hermann Josef
Published: August 2023

Background: Proximal humeral fractures (PHFs) are common injuries that can lead to significant functional impairment. This retrospective cohort study aimed to evaluate the clinical outcomes and complications associated with the use of the Targon PH+ (Fa. Aesculap, Germany) intramedullary nail for the treatment of PHFs. Methods: A subgroup consisting of 70 patients with a mean follow-up of 4.91 years out of 479 patients who underwent treatment with the Targon PH+ intramedullary nail for PHFs at a single center between 2014 and 2021 were included. Patient-reported outcome measures (PROMs) and health-related quality of life (HRQoL) were assessed using validated German versions of the Disabilities of the Arm, Shoulder, and Hand (DASH) questionnaire, American Shoulder and Elbow Surgeons (ASES) score, Oxford Shoulder Score (OSS), and EuroQol 5-Dimension 5-Level (EQ-5D-5L). Radiographic assessment was performed using pre- and postoperative imaging. Results: Among the 70 patients of the subgroup who completed follow-up, 21.4% experienced complications, including major complications in 15.7% of cases, all of which were revised (revision rate of 15.7%). Anatomical reduction was achieved in 48,5% of cases. The mean DASH, ASES, and OSS scores were 25.4 ± 22.0, 76.2 ± 21.1, and 38.8 ± 10.3, respectively. Significant correlations were observed among the PROMs, indicating their convergent validity. Additionally, a significant correlation of all used PROMs and patient well-being (HRQoL) was observed. Severe complications and revisions were associated with significantly lower ASES scores (−11.1%, p = 0.013). There was a tendency for PROM scores to slightly decline with increasing fracture complexity, although this trend did not reach statistical significance. Our findings indicate that patients over the age of 65 years tend to exhibit lower scores in PROMs and HRQoL measures. Conclusion: The use of the Targon PH+ intramedullary nail for the treatment of PHFs resulted in satisfactory clinical outcomes and acceptable complication and revision rates. The PROMs and HRQoL measures indicated varying levels of disability and symptoms, with major complications, revision surgery, and age negatively impacting shoulder function after midterm follow-up.
Spatiotemporal Analysis and Prediction of Urban Land Use/Land Cover Changes Using a Cellular Automata and Novel Patch-Generating Land Use Simulation Model: A Study of Zhejiang Province, China
Authors: Koko Auwalu Faisal , Han Zexu , Wu Yue , Zhang Siyuan , Ding Nan , Luo Jiayang
Published: August 2023

Urban land use/land cover (LULC) monitoring and prediction are vital for understanding the spatiotemporal change dynamics of future land uses. They provide the necessary data for effectively planning and managing natural land resources. In this study, we analyzed and simulated the changes in urban LULC within Zhejiang Province, a region in China experiencing rapid urbanization. By exploring the historical change dynamics of the region, we observed substantial transformations in the extent of built-up areas, forests, and agricultural land from 1995 to 2020. Specifically, the study area witnessed the expansion in urban built-up areas by approximately 6126.93 km2, while forests and agricultural land witnessed decreases of 3252.47 km2 and 2885.13 km2, respectively. To predict the study area’s future LULC, a cellular automata (CA) model was utilized in combination with an advanced patch-generating land use simulation (PLUS) model. This integrated approach allowed for multiple land use predictions based on different scenarios. Under the baseline scenario (BLS), it was projected that the area of urban expansion in Zhejiang Province would be approximately 4501.62 km2. However, under the scenario of cultivated land and ecological protection, i.e., CLPS and EPS, urban growth was observed to be 538.64 km2 and 1776.16 km2, respectively. These findings indicate that the extent of built-up area development in Zhejiang Province is significantly reduced when the CLPS and EPS are implemented in comparison to the BLS. Therefore, policy interventions are crucial to protect agricultural land and conserve ecological areas. This research provides the scientific data needed for proper planning and serves as reference data for other regions with similar rapid urbanization.
Properties of Anti-Invariant Submersions and Some Applications to Number Theory
Authors: Hakami Ali H. , Siddiqi Mohd. Danish
Published: August 2023

In this article, we investigate anti-invariant Riemannian and Lagrangian submersions onto Riemannian manifolds from the Lorentzian para-Sasakian manifold. We demonstrate that, for these submersions, horizontal distributions are not integrable and their fibers are not totally geodesic. As a result, they are not totally geodesic maps. The harmonicity of such submersions is also examined. We specifically prove that they are not harmonic when the Reeb vector field is horizontal. Finally, we provide an illustration of our findings and mention some number-theoretic applications for the same submersions.
Accuracy Is Not Enough: Optimizing for a Fault Detection Delay
Authors: Šprogar Matej , Verber Domen
Published: August 2023

This paper assesses the fault-detection capabilities of modern deep-learning models. It highlights that a naive deep-learning approach optimized for accuracy is unsuitable for learning fault-detection models from time-series data. Consequently, out-of-the-box deep-learning strategies may yield impressive accuracy results but are ill-equipped for real-world applications. The paper introduces a methodology for estimating fault-detection delays when no oracle information on fault occurrence time is available. Moreover, the paper presents a straightforward approach to implicitly achieve the objective of minimizing fault-detection delays. This approach involves using pseudo-multi-objective deep optimization with data windowing, which enables the utilization of standard deep-learning methods for fault detection and expanding their applicability. However, it does introduce an additional hyperparameter that needs careful tuning. The paper employs the Tennessee Eastman Process dataset as a case study to demonstrate its findings. The results effectively highlight the limitations of standard loss functions and emphasize the importance of incorporating fault-detection delays in evaluating and reporting performance. In our study, the pseudo-multi-objective optimization could reach a fault-detection accuracy of 95% in just a fifth of the time it takes the best naive approach to do so.
Common Fixed Point Theorems for Novel Admissible Contraction with Applications in Fractional and Ordinary Differential Equations
Authors: Atiponrat Watchareepan , Varnakovida Pariwate , Chanthorn Pharunyou , Suebcharoen Teeranush , Charoensawan Phakdi
Published: August 2023

In our work, we offer a novel idea of contractions, namely an (α,β,γ)P−contraction, to prove the existence of a coincidence point and a common fixed point in complete metric spaces. This leads us to an extension of previous results in the literature. Furthermore, we applied our acquired results to prove the existence of a solution for ordinary and fractional differential equations with integral-type boundary conditions.
From Threat to Opportunity: Harnessing the Invasive Carpobrotus edulis (L.) N.E.Br for Nutritional and Phytotherapeutic Valorization Amid Seasonal and Spatial Variability
Authors: Pereira Catarina , Neng Nuno , Custódio Luísa
Published: August 2023

Carpobrotus edulis (L.) N.E.Br. (Hottentot-fig) is a problematic invasive species found in coastal areas worldwide. Mechanical removal is a common control method, leaving the removed biomass available as a possible source of natural phytochemicals with prospective commercial applications. While the Hottentot-fig’s vegetative organs have been studied previously, this work establishes for the first time a seasonal and spatial comparative analysis of its nutritional, chemical, and bioactivity profiles (in three locations over four seasons). Proximate and mineral contents were assessed, along with its phenolic composition and in vitro antioxidant and anti-inflammatory properties. Hottentot-fig’s biomass offered a good supply of nutrients, mainly carbohydrates, proteins, and minerals, with a tendency for higher concentrations of the most relevant minerals and proteins in autumn and winter, and in plants from sites A (Ria de Alvor lagoon) and B (Ancão beach). The extracts were rich in polyphenolics, with higher levels in spring and summer, especially for luteolin-7-O-glucoside and salicylic and coumaric acids. The extracts were also effective antioxidants, with stronger radical scavenging activities in spring and summer, along with anti-inflammatory properties. Our results suggest that the usually discarded plant material of this invasive halophyte could be valuable as a source of natural products with potential biotechnological applications in the food and nutraceutical industries.
Effect of Hydroxyl-Containing Fragments on the Structure and Properties of Membrane-Forming Polyamide-Imides
Authors: Kononova Svetlana V. , Lebedeva Galina K. , Gubanova Galina N. , Kruchinina Elena V. , Vlasova Elena N. , Afanas’eva Nadezhda V. , Popova Elena N. , Volkov Anatoly Ya. , Bykova Elena N. , Zakharova Natalya V.
Published: August 2023

The structural features and thermophysical and transport properties of dense nonporous membranes of the casting type from (co)polyamide-imides synthesized by the polycondensation of the diacid chloride of 2-(4-carboxyphenyl)-1,3-dioxoisoindoline-5-carboxylic acid and diamines 5,5′-methylene-bis (2-aminophenol) (DADHyDPhM) and 4,4′-methylenebis(benzeneamine) (DADPhM), taken in molar ratios of 7:3, 1:1, and 3:7, have been studied. The effect of hydroxyl-containing modifying fragments of dihydroxy diphenylmethane introduced in various amounts into the main polymer chain on the pervaporation properties of the formed films is discussed. It has been shown that the presence of the residual solvent N-methyl-2-pyrrolidone in the films not only has a plasticizing effect on the characteristics of film membranes but also promotes the preferential transmembrane transport of polar liquids, primarily methanol (permeation rate over 2 kg for a copolymer with a ratio of DADHyDPhM:DADPhM = 7:3). The removal of the residual solvent from the polymer film, both thermally (heating to 200 °C) and by displacement with another solvent as a result of sequential pervaporation, led to a significant decrease in the rate of transfer of polar liquids and a decrease in the selectivity of the membrane. However, the dehydrocyclization reaction resulted in more brittle films with low permeability to penetrants of different polarities. The results of our comprehensive study made it possible to assume the decisive influence of structural changes in membranes occurring in connection with the competitive formation of intra- and intermolecular hydrogen bonds.
Features of Structure and Properties of Lap-Welded Joints of Aluminum Alloy Al–4Cu–1Mg with Titanium Alloy Ti–6Al–4V, Obtained by Friction Stir Welding
Authors: Ivanov Alexey , Chumaevskii Andrey , Amirov Alihan , Utyaganova Veronika , Savchenko Nikolay , Rubtsov Valery , Tarasov Sergei
Published: August 2023

Lap-welded joints between Ti–6Al–4V and Al–4Cu–1Mg were obtained using water-cooling-bath friction stir welding at different FSW tool rotation rates. The increase in the tool rotation rate from 350 to 375 RPM leads to better plasticization of the titanium alloy, elimination of stir zone defects, better interlocking and bonding with the aluminum alloy as well as the formation of intermetallic Al–Ti compounds (IMC), preferentially of the Al3Ti type. Shear-loading testing showed the best result was at the level of 3000 N with 2 mm of displacement. Increasing the FSW tool rotation rate to 400 and 450 RPM resulted in the formation of more IMCs, which had a detrimental effect on both maximum load and displacement achieved in the shear tests.
Comparative Analysis of Prokaryotic Extracellular Vesicle Proteins and Their Targeting Signals
Authors: Stathatos Ilias , Koumandou Vassiliki Lila
Published: August 2023

Prokaryotic extracellular vesicles (EVs) are vesicles that bud from the cell membrane and are secreted by bacteria and archaea. EV cargo in Gram-negative bacteria includes mostly periplasmic and outer membrane proteins. EVs are clinically important as their cargo can include toxins associated with bacterial virulence and toxicity; additionally, they have been proposed as efficient vaccine agents and as the ancestors of the eukaryotic endomembrane system. However, the mechanistic details behind EV cargo selection and release are still poorly understood. In this study, we have performed bioinformatics analysis of published data on EV proteomes from 38 species of bacteria and 4 archaea. Focusing on clusters of orthologous genes (COGs) and using the EggNOG mapper function, we have identified cargo proteins that are commonly found in EVs across species. We discuss the putative role of these prominent proteins in EV biogenesis and function. We also analyzed the published EV proteomes for conserved signal sequences and discuss the potential role of these signal sequences for EV cargo selection.
Effects of Controlled Ozone Exposure on Circulating microRNAs and Vascular and Coagulation Biomarkers: A Mediation Analysis
Authors: Chen Hao , Masood Syed , Rappold Ana G. , Diaz-Sanchez David , Samet James M. , Tong Haiyan
Published: August 2023

Exposure to ozone (O3) is associated with adverse respiratory and cardiovascular outcomes. Alterations in circulating microRNAs (miRNAs) may contribute to the adverse vascular effects of O3 exposure through inter-cellular communication resulting in post-transcriptional regulation of messenger RNAs by miRNAs. In this study, we investigated whether O3 exposure induces alterations in circulating miRNAs that can mediate effects on downstream vascular and coagulation biomarkers. Twenty-three healthy male adults were exposed on successive days to filtered air and 300 ppb O3 for 2 h. Circulating miRNA and protein biomarkers were quantified after each exposure session. The data were subjected to mixed-effects model and mediation analyses for the statistical analyses. The results showed that the expression level of multiple circulating miRNAs (e.g., miR-19a-3p, miR-34a-5p) was significantly associated with O3 exposure. Pathway analysis showed that these miRNAs were predictive of changing levels of downstream biomarkers [e.g., D-dimer, C-reactive protein, tumor necrosis factor α (TNFα)]. Mediation analysis showed that miR-19a-3p may be a significant mediator of O3-exposure-induced changes in blood TNFα levels [0.08 (0.01, 0.15), p = 0.02]. In conclusion, this preliminary study showed that O3 exposure of healthy male adults resulted in changes in circulating miRNAs, some of which may mediate vascular effects of O3 exposure.
Space–Time Patterns of Poultry Pathogens in the USA: A Case Study of Ornithobacterium rhinotracheale and Pasteurella multocida in Turkey Populations
Authors: Campler Magnus R. , Hashish Amro , Ghanem Mostafa , El-Gazzar Mohamed M. , Arruda Andréia G.
Published: August 2023

Respiratory infections caused by Ornithobacterium rhinotrachealis (ORT) and Pasteurella multocida (PM) bacteria are significant threats to the poultry industry by causing economic losses and welfare issues. Due to characterization difficulties and underutilization of epidemiological tools, description of the spatio-temporal spread of these diseases in the field is limited. The objectives of this retrospective observational cross-sectional study were to (a) investigate the existence of space–time clusters (hotspots); and (b) investigate the association between genetic similarity and spatial proximity for both pathogens using molecular typing and a recently developed Core-Genome Multilocus Sequencing Typing (cgMLST) scheme. ORT (n = 103) and PM (n = 69) isolates from confirmed disease outbreaks from one commercial company between 2013 and 2021 were obtained from a veterinary diagnostic laboratory, characterized using a cgMLST scheme and visualized using a minimum spanning tree. Spatio-temporal cluster analysis using SaTScanTM and a Spearman’s rank correlation were performed to investigate clustering and any association between allelic diversity and geospatial distance. The cgMLST sequencing revealed three allelic clusters for ORT and thirteen clusters for PM. The spatio-temporal analysis revealed two significant clusters for PM, one with a 259.3 km cluster containing six cases between May and July 2018 and a 9 km cluster containing five cases between February 2019 and February 2021. No spatio-temporal clusters were found for ORT. A weak negative correlation between allelic diversity and geospatial distance was observed for ORT (r = −0.04, p < 0.01) and a weak positive correlation was observed for PM (r = 0.11, p < 0.01). This study revealed regional spatio-temporal clusters for PM in commercial turkey sites between 2018 and 2021 and provided additional insight into bacterial strain subgroups and the geographical spread of ORT and PM over time.
Indoor Bacterial and Fungal Burden in “Moldy” versus “Non-Moldy” Homes: A Case Study Employing Advanced Sequencing Techniques in a US Metropolitan Area
Authors: Chauhan Bhavin V. , Higgins Jones Daleniece , Banerjee Goutam , Agrawal Saumya , Sulaiman Irshad M. , Jia Chunrong , Banerjee Pratik
Published: August 2023

The presence of fungi in the indoor environment is associated with allergies and other respiratory symptoms. The aim of this study was to use sequencing and molecular methods, including next-generation sequencing (NGS) approaches, to explore the bacterial and fungal communities and their abundance in the indoor environment of houses (n = 20) with visible “moldy” (HVM) and nonvisible “non-moldy” (HNM) in Memphis, TN, USA. Dust samples were collected from air vents and ground surfaces, and the total DNA was analyzed for bacteria and fungi by amplifying 16S rRNA and ITS genes on the Illumina Miseq. Results indicated that Leptosphaerulina was the most abundant fungal genus present in the air vent and ground samples from HNM and HVM. At the same time, the most abundant bacterial genera in the air vent and ground samples were Propionibacterium and Streptococcus. The fungi community diversity was significantly different in the air vent samples. The abundance of fungal species known to be associated with respiratory diseases in indoor dust samples was similar, regardless of the visibility of fungi in the houses. The existence of fungi associated with respiratory symptoms was compared with several parameters like dust particulate matter (PM), CO2 level, temperature, and humidity. Most of these parameters are either positively or negatively correlated with the existence of fungi associated with respiratory diseases; however, none of these correlations were significant at p = 0.05. Our results indicate that implementing molecular methods for detecting indoor fungi may strengthen common exposure and risk assessment practices.
Drug Prescriptions during Pregnancy in Lombardy: Temporal Trends and the Impact of the Onset of the COVID-19 Pandemic
Authors: Esposito Giovanna , Cantarutti Anna , Franchi Matteo , Corrao Giovanni , Parazzini Fabio
Published: August 2023

This population-based study aimed at providing an overview of drug prescription patterns during pregnancy in the Italian region of Lombardy from 2010 to 2020. The cohort consisted of 780,075 deliveries identified from the regional healthcare utilization databases. The prevalence of drugs’ dispensed prescriptions was estimated as the proportion of pregnant women with at least one prescription out of the total deliveries over the entire pregnancy and by trimester. Drugs were classified according to the Anatomical Therapeutic Chemical code. In addition, interrupted time series analysis was conducted to investigate temporal trends of antibiotics’ use during the onset of the COVID-19 pandemic. A total of 497,515 women (63.8%) used at least a drug, including vitamins and minerals, at some point during pregnancy. Vitamins, minerals, and anti-anaemic preparations were prescribed in 20.8%, 13.3%, and 18.3% of deliveries over the trimesters of pregnancy. Folic acid was the most prescribed drug, with about one woman out of four, followed by iron preparations, progestogen, and antibiotics (prescription rate, respectively: 15.9%, 10.2%, and 9.8%). A decreasing trend in the dispensing of antibiotics emerged during the entire study period; however, a significant further decrease following the spread of the pandemic was observed. Further evidence is needed to monitor the use of drugs during pregnancy, determinants, and implications.
Generation of Second-Order Sideband through Nonlinear Magnetostrictive Interaction
Authors: Yang Lei , Wang Bao , Xiong Hao
Published: August 2023

Nonlinear interaction between the magnon mode and the mechanical mode in a magnomechanical system is treated analytically where the magnon mode is coherently driven by a bichromatic microwave drive field consisting of a strong pumping field and a weak probe field and that works within a perturbative regime. Using experimentally achievable parameters, we show that the magnonic second-order sideband is generated and can be considerably enhanced by increasing the power of the pumping field. The suppression of the magnonic second-order sideband generation at the resonance point is discussed. Furthermore, the efficiency of magnonic second-order sideband generation can be well controlled by adjusting the applied bias magnetic field strength, which is a particular feature compared to the optical second-order sideband. In addition to offering insights into the magnomechanical nonlinearity, the present results have the potential to pave the way for exploring practical applications for achieving high-precision measurement in magnonics.
Dependence of the Michelson Interferometer-Based Membrane-Less Optical Microphone–Photoacoustic Spectroscopy Gas-Sensing Method on the Fundamental Parameters of a Photoacoustic Gas Cell
Authors: Wang Shuchao , Yetisen Ali K. , Wang Kun , Jakobi Martin , Koch Alexander W.
Published: August 2023

This article presents a mathematical model of the Michelson interferometer (MI)-based membrane-less optical microphone (MeoM)–photoacoustic spectroscopy (MeoM–PAS) method, which is also referred to as MI-based photoacoustic interferometry (PAI), for gas-sensing applications in complex and adverse environments, as it offers a completely static measurement system and the separation of a photoacoustic (PA) gas cell from the measuring system. It also investigates the dependence of this method on the fundamental parameters of a cubical PA gas cell using axial PA signals. The results indicate that the phase of the method is a sine function of the distance between the two light beams and a power exponent of the cell length, the cell height, and the distance between the excitation source and the nearest light beam, under the condition that the PA gas cell is resonant and that the excitation source is at the position of the peak or valley of the PA signals. It is at its maximum when the distance between the two light beams is approximately half the wavelength of the PA signals under the same conditions. In addition, the dependence of a PA gas cell using non-axial PA signals is described under the conditions that the PA gas cell is resonant, which is consistent with the changing aforementioned parameters for the distance between the two light beams, the cell length and height, and the distance between the excitation source and the nearest light beam. Furthermore, the selection of five common materials (aluminum, brass, glass, quartz, and stainless steel) for the PA gas cell is discussed under the influence of temperature fluctuations outside the PA gas cell, noise inside and outside the PA gas cell, as well as thermal and viscous losses inside the PA gas cell. The results indicate that quartz and stainless steel are promising options. Finally, the parameters related to the sensitivity enhancement of the method are analyzed using mathematical models, where the sensitivity of the method can be theoretically enhanced by reducing the dimensions of the PA gas cell.
Effects of Spring Drought and Nitrogen Addition on Productivity and Community Composition of Degraded Grasslands
Authors: Li Shaoning , Lu Shaowei , Li Xiaohui , Hou Xingchen , Zhao Xi , Xu Xiaotian , Zhao Na
Published: August 2023

To explore whether there were differences among the patterns of response of grasslands with different levels of degradation to extreme drought events and nitrogen addition, three grasslands along a degradation gradient (extremely, moderately, and lightly degraded) were selected in the Bashang area of northern China using the human disturbance index (HDI). A field experiment with simulated extreme spring drought, nitrogen addition, and their interaction was conducted during the growing seasons of 2020 and 2021. The soil moisture, aboveground biomass, and composition of the plant community were measured. The primary results were as follows. (1) Drought treatment caused soil drought stress, with moderately degraded grassland being the most affected, which resulted in an 80% decrease in soil moisture and a 78% decrease in aboveground biomass. The addition of nitrogen did not mitigate the impact of drought. Moreover, the aboveground net primary production (ANPP) in 2021 was less sensitive to spring drought than in 2020. (2) The community composition changed after 2 years of drought treatment, particularly for the moderately degraded grasslands with annual forbs, such as Salsola collina, increasing significantly in biomass proportion, which led to a trend of exacerbated degradation (higher HDI). This degradation trend decreased under the addition of nitrogen. (3) The variation in drought sensitivities of the ANPP was primarily determined by the proportion of plants based on the classification of degradation indicators in the community, with higher proportions of intermediate degradation indicator species exhibiting more sensitivity to spring drought. These findings can help to provide scientific evidence for the governance and restoration of regional degraded grassland under frequent extreme weather conditions.