bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Machine Learning for Shale Oil and Gas “Sweet Spots” Prediction
Authors: Wang Hongjun , Guo Zekun , Kong Xiangwen , Zhang Xinshun , Wang Ping , Shan Yunpeng
Published: May 2024

With the continuous improvement of shale oil and gas recovery technologies and achievements, a large amount of geological information and data have been accumulated for the description of shale reservoirs, and it has become possible to use machine learning methods for “sweet spots” prediction in shale oil and gas areas. Taking the Duvernay shale oil and gas field in Canada as an example, this paper attempts to build recoverable shale oil and gas reserve prediction models using machine learning methods and geological and development big data, to predict the distribution of recoverable shale oil and gas reserves and provide a basis for well location deployment and engineering modifications. The research results of the machine learning model in this study are as follows: ① Three machine learning methods were applied to build a prediction model and random forest showed the best performance. The R2 values of the built recoverable shale oil and gas reserves prediction models are 0.7894 and 0.8210, respectively, with an accuracy that meets the requirements of production applications; ② The geological main controlling factors for recoverable shale oil and gas reserves in this area are organic matter maturity and total organic carbon (TOC), followed by porosity and effective thickness; the main controlling factor for engineering modifications is the total proppant volume, followed by total stages and horizontal lateral length; ③ The abundance of recoverable shale oil and gas reserves in the central part of the study area is predicted to be relatively high, which makes it a favorable area for future well location deployment.
Distribution Dynamics of Diplopanax stachyanthus Hand.-Mazz. (Mastixiaceae) and Its Implications in Relict Mastixioid Flora Conservation
Authors: Chen Menglin , Yang Yongjingwen , Lin Lin , Tan Yunhong , Deng Min , Zuo Yunjuan
Published: May 2024

Climate is a key driver shaping the distribution pattern of organisms. Cenozoic climate change has led to extensive biota turnover. Untangling the distribution dynamics of a representative lineage of flora can provide deep insights into biodiversity conservation. Diplopanax is a notable relict lineage of the Tertiary mastixioid flora with abundant fossils in the Northern Hemisphere. Diplopanax stachyanthus Hand.-Mazz. is a representative relic lineage of the mastixioid flora, which was once widespread in the Northern Hemisphere of the early Tertiary period, but with only endemic distribution in the (sub)tropical humid forests of East Asia. It offers a unique chance to understand how climatic drivers shape the Boreotropical flora. In this research, we investigated the distribution dynamics of D. stachyanthus at the last glacial maximum (LGM), mid-Holocene (MH), current, and three periods of the future (2041–2060, 2061–2080, and 2081–2100) at four shared socio-economic emissions scenarios pathways. Our results indicated that the Precipitation of the Wettest Quarter (32.6%), the Precipitation of the Driest Quarter (21.2%), and the Precipitation of the Coldest Quarter (17.3%) are the key factors affecting its distribution. The current high suitable distribution areas are primarily in southern China and northern Indo-China. The enforced winter monsoon seasons in East Asia since the late Pliocene period are the key climatic drivers reducing its once widespread distribution in the Northern Hemisphere. Under future scenarios, centroid transfer analysis suggests that its distribution center will shift southwestward, but the potentially suitable habitats in the coastal regions of southern China and northern Indo-China will be lost. These coastal populations should be prioritized for ex situ conservation. Expanding the nature reserve within its long-term stable distribution range in southwest China is an effective strategy for the in situ conservation of the ancient mastixioid flora.
Physical and Mechanical Properties of Fiberboard Made of MDF Residues and Phase Change Materials
Authors: Rodríguez Gustavo E. , Bustos Ávila Cecilia , Cloutier Alain
Published: May 2024

The wood-based panel industry is experiencing an excessive accumulation of solid residues from the production of medium-density fiberboard (MDF) panels and moldings. It is possible to create new MDF products with acceptable physical and mechanical properties by revaluing MDF residues. Additionally, those products’ thermal properties can be improved by incorporating phase change materials (PCMs). This study aims to develop a wood-based fiberboard made of MDF residues, capable of storing thermal energy. Two types of PCMs (liquid and microencapsulated), two PCM ratios (2% and 6%), and two types of adhesives (urea-formaldehyde and phenol-formaldehyde) were used to produce eight different types of panels. The vertical density profile, thickness swelling, water absorption, internal bond (IB), and static bending properties—modulus of elasticity (MOE) and modulus of rupture (MOR)—were determined for each panel type. The specific heat of the panels was also determined. The results show the panels’ densities were greater than 700 kg/m3. Thickness swelling in water improved by 23% compared to the reference value of the control panel PCMs after PCM incorporation. The highest IB value was 1.30 MPa, which is almost three times the minimum required by regulation standards. The incorporation of PCMs reduced the panels’ bending properties compared to the properties of the control panels. Even though the values obtained are sufficient to comply with the minimum values set out in ANSI standard A208.2 with an MOE value of 2072.4 MPa and the values obtained are sufficient to comply with the minimum standards with an MOE value of 2072.4 MPa and an MOR value of 16.4 MPa, when microencapsulated PCM is used, the specific heat of the panels is increased by more than 100% over that of the control panels. This study developed fiberboards with adequate physical and mechanical properties and capable of storing thermal energy.
Optimizing Variational Problems through Weighted Fractional Derivatives
Authors: Almeida Ricardo
Published: May 2024

In this article, we explore a variety of problems within the domain of calculus of variations, specifically in the context of fractional calculus. The fractional derivative we consider incorporates the notion of weighted fractional derivatives along with derivatives with respect to another function. Besides the fractional operators, the Lagrange function depends on extremal points. We examine the fundamental problem, providing the fractional Euler–Lagrange equation and the associated transversality conditions. Both the isoperimetric and Herglotz problems are also explored. Finally, we conclude with an analysis of the variational problem, incorporating fractional derivatives of any positive real order.
Duodenal Adenocarcinoma: The Relationship between Type of Surgery and Site of Recurrence in a Spanish Cohort
Authors: Blanco-Fernández Gerardo , Aparicio-López Daniel , Villodre Celia , Jaén-Torrejimeno Isabel , Alcázar López Cándido F , López-Guerra Diego , Serradilla-Martín Mario , Ramia José M.
Published: May 2024

We present a multicenter retrospective study of patients undergoing surgery for duodenal adenocarcinoma, from January 2010 to August 2020, in order to determine the epidemiological characteristics and the oncological results after surgical resection obtained in this rare tumor. Variables: demographics; tumor location; surgical intervention and immediate postoperative period; and post-surgical follow-up information, such as recurrence, overall survival (OS), and disease-free survival (DFS). A total of 32 patients underwent surgery. The median age was 69.74 years (IQR 60.47–79.09) and the male/female distribution was 3:1. The surgeries performed were: pancreaticoduodenectomy (PD) in 16 (50%) patients, segmental resection in 13 (40.6%), and the local excision of the lesion in three (9.4%). The R0 rate was higher in PD (86.7% vs. 42.9%; p = 0.013). The OS and DFS rate at one, three and five years was 95%, 70%, and 60% and 86%, 55%, and 48%, respectively. There was a greater trend towards recurrence in patients who did not undergo PD (53.8% vs. 25%; p = 0.14) and conservative surgery seemed to be associated with more local recurrence than PD (57.1% vs. 33.3%; p = 0.49). PD and limited resection are both valid options in the cases of non-ampullary duodenal adenocarcinoma, although PD presented lower rates of loco-regional recurrence.
Parallel Narratives: Trauma, Relationality, and Dissociation in Psychoanalysis and Realist Fiction
Authors: Becker Mona , Sjöström C. Christina
Published: May 2024

The reciprocal relationship between cultural trauma studies and psychoanalytic discourse on the one hand, and trauma studies and fictional representations of trauma on the other, has been commented on by scholars within the field of literary studies. What connects the representation of trauma in cultural trauma theory, trauma fiction, and psychoanalysis is that it is regarded as something that overwhelms an individual’s capacities for processing and functioning. However, while cultural trauma theory has come under scrutiny for prioritizing too narrow a view of trauma and its representations, the considerable critiques of and revisions to Freud’s theories, developed in the 1980/90s, have been mostly ignored by cultural trauma theorists. In this interdisciplinary article, we draw on relational psychoanalytic perspectives to demonstrate how relational revisions to psychoanalytic theory and techniques, as well as views on dissociation, can offer new perspectives for approaching literary works of fiction, such as the realist novel, which engage with the subject of trauma outside of established trauma conventions. We demonstrate that trauma novels by Lisa Appignanesi and Aminatta Forna parallel these revisions to psychoanalytic theory and techniques, allowing for a more pluralistic and nuanced representation of responses to trauma and suffering.
Factors Associated with Evidence-Based Practice Competencies among Taiwanese Nurses: A Cross-Sectional Study
Authors: Cheng Li-Chuan , Chen Chia-Jung , Lin Shih-Chun , Koo Malcolm
Published: May 2024

Evidence-based practice (EBP) is an essential component of healthcare practice that ensures the delivery of high-quality care by integrating the best available evidence. This study aimed to explore factors influencing EBP among nursing professionals in Taiwan. A cross-sectional survey study was conducted with 752 registered nurses and nurse practitioners recruited from a regional teaching hospital in southern Taiwan. EBP competency was evaluated using the Taipei Evidence-Based Practice Questionnaire (TEBPQ). The results showed that participation in evidence-based courses or training within the past year had the strongest association with EBP competencies (Std. B = 0.157, p < 0.001). Holding a graduate degree (Std. B = 0.151, p < 0.001), working in gynecology or pediatrics (Std. B = 0.126, p < 0.001), searching the literature in electronic databases (Std. B = 0.072, p = 0.039), and able to read academic articles in English (Std. B = 0.088, p = 0.005) were significantly associated with higher TEBPQ scores. Younger age (Std. B = −0.105, p = 0.005) and male gender (Std. B = 0.089, p = 0.010) were also identified as factors contributing to higher EBP competencies. The study highlights the importance of ongoing professional development, including EBP training and language proficiency, in enhancing EBP competencies among nursing professionals in Taiwan.
Oral Health Status of Healthcare Workers in Ilembula/Tanzania during the COVID-19 Condition
Authors: Bensel Tobias , Megiroo Simon , Kronenberg Werner , Bömicke Wolfgang , Ulrichs Timo , Hinz Sebastian
Published: May 2024

The challenge of reduced dental treatment and education infrastructure in the Tanzanian highlands affects the oral health situation of both the general population and local healthcare workers. The aim of this study was to investigate the oral health status of healthcare workers at Ilembula Lutheran Hospital (ILH), Tanzania, during the COVID-19 pandemic. In total, 134 healthcare workers (62 women, 72 men; mean age 36.48 ± 9.56 years, range 19–59 years; median age 35.00 years) participated in this cross-sectional study, conducted from 12 February to 27 February. A dental examiner trained in oral health screening performed the oral health data collection. Data collection was performed by probability sampling using the Ilembula Data Collection Form—Oral Health (IDCF-Oral Health) questionnaire distributed in paper form. Ethical approval was obtained from the National Institute for Medical Research/Tanzania. The decayed, missing, and filled teeth (DMF/T) index proposed by the World Health Organization (WHO) was used with the associated caries measurement method and the simplified oral hygiene index (OHI-S). Details regarding edentulism, nutritional habits, and socio-economic factors were collected. Statistical analysis was performed using linear regression (α = 0.05). The average DMF-T index was 3.33 ± 0.82, with age, gender, meal frequency, and soft drink consumption significantly influencing the index. No evidence of dental plaque was detected in 43.3% of the participants. Of the participants, 32.8% required prosthetic treatment (Kennedy Class III), while 16.4% needed it for acute malocclusions. Oral hygiene products were used in 97% of cases. A total of 35.8% of the participants had an OHI-S score of up to 1.0, with (p < 0.001) age and (p < 0.001) sex having a significant influence on the index. The current oral health situation of healthcare workers at ILH shows a moderate need for restorative and prosthetic treatment in rural Tanzania. Despite the COVID-19 pandemic, there was no change in the need for dental treatment, which may be explained by the generally restricted access to dental healthcare in the investigated region. The development of an interdisciplinary oral health prophylaxis system could help to reduce the need for future treatments.
Patient-Orientated Evaluation of Treatment of Non-Melanoma Skin Cancer with Rhenium-188 Compared to Surgery
Authors: Krönert Maila I. C. , Schwarzenböck Sarah M. , Kurth Jens , Heuschkel Martin , Krause Bernd J. , Emmert Steffen , Tietze Julia K.
Published: May 2024

Background: Non-melanoma skin cancers (NMSCs) are responsible for up to one-third of all human malignancies. Surgery is usually the treatment of choice, but patients often experience pain during the procedure. Topical rhenium-188 resin skin cancer treatment (RSCT) may be a valid therapeutic alternative. Methods: In this monocentric pilot study, 19 patients suffering from NMSC were treated with RSCT. Most of these patients had also experienced surgery, either because they developed a new NMSC in aftercare, or they had suffered previously from NMSC. Three RSCT-treated patients, who had no exposure to surgery so far, were paired with three matched patients, who had received surgery. We sought to evaluate and compare the patients’ experience with both treatments. A questionnaire assessed patients’ perceptions regarding side effects, aesthetic outcomes, wound care, fear of complications, and personal treatment preferences. Patients evaluated the different parameters of their either RSCT- or surgery-treated lesions on a scale from 0–10. Results: Patients were more afraid of complications before surgery than before RSCT (p = 0.04). Treatment with RSCT caused significantly less pain on treatment day (mean 0.56) than surgery (mean 2.32) (0 no pain, 10 maximum pain) (p = 0.02) and 14 days after the procedure (mean 0.89 versus mean 2.47) (p = 0.02). On day 14, RSCT-treated lesions were also significantly less itchy (mean 0.34) than after surgery (mean 1.50). Most patients were very satisfied with the aesthetic outcome after both RSCT (mean 8.42) and surgery (mean 8.31) (p = 0.89). In the case of a new NMSC, the majority of patients who experienced both treatments would rather be treated primarily with RSCT (44%) or would consider both options (31%); only 19% preferred surgery. Conclusion: Patients evaluated RSCT as less painful than surgery. The aesthetic outcomes of both treatments were comparable. For pain-sensitive patients, RSCT might be a preferable treatment option.
Digital Apps to Improve Mobility in Adults with Neurological Conditions: A Health App-Focused Systematic Review
Authors: Rendell Reem , Pinheiro Marina , Wang Belinda , McKay Fiona , Ewen Ashleigh , Carnegie Catherine , Tikomaidelana Erin , Fattah Zino , Hassett Leanne
Published: May 2024

The provision of mobility exercises through a smartphone application (app) for people undertaking neurological rehabilitation may improve mobility outcomes. However, it is difficult for clinicians and consumers to select high-quality, appropriate apps. This review aimed to identify (1) which mobile health (mHealth) apps are suitable for prescribing mobility exercises for adults with neurological health conditions, (2) how well these apps incorporate telehealth strategies, and (3) how well these apps rate in terms of quality and capacity for behaviour change. The Australian Apple iTunes Store was systematically searched, by using a search code and manually, for apps suitable for training mobility in neurological rehabilitation. Additional searches were conducted in known app repositories and for web-based apps. Trained reviewers extracted data from the included apps, including population-specific characteristics; quality, by using the Mobile App Rating Scale (MARS); and behaviour change potential, by using the App Behaviour Change Scale (ABACUS). The included apps (n = 18) provided <50 to >10,000 exercises, many incurred a subscription fee (n = 13), and half included telehealth features. App quality was moderate (mean MARS score of 3.2/5 and SD of 0.5), and potential for behaviour change was poor (mean ABACUS score of 5.7/21 and SD of 2.1). A limited number of high-quality apps are available for the prescription of mobility exercises in people with neurological conditions.
Pilot Testing of Useful Tools’ Validity for Frailty Assessment in Greece: Translated PRISMA-7 Tool, Modified Fried Criteria and Clinical Frailty Scale
Authors: Soulis George , Kyriakopoulou Efstathia , Leventouri Aristea , Zigkiri Eleni , Efthymiou Vasiliki , Kentros Zikos , Koutsouri Anastasia
Published: May 2024

The importance of frailty in older people is getting constant recognition as an important aspect both in terms of public health, as well as at a personal level, for the appropriate management of an older person’s health condition. This is reflected by the continuously increasing number of research studies carried out in several settings across different countries. Sometimes, this is very solid, but in other cases, there is a considerable gap in terms of accurate and well-grounded documentation of frailty status. This is the case in Greece, where we are missing clinically validated tools to approach frailty. We are missing frailty screening tools, such as, for instance, Program of Research on Integration of Services for the Maintenance of Autonomy 7 (PRISMA 7), the gold standard tool of Fried criteria, is somehow problematic since the question referring to physical activity originates from a questionnaire that has not been translated and validated, while Clinical Frailty Scale (CFS) has been validated for translation but not for the capacity to detect frailty. The aim of this study is to validate these tools for their accuracy to detect frailty by using a measurable index of frailty, previously proposed for use in clinical studies: the Short Physical Performance Battery (SPPB). Seventy-four male and female participants (mean age 80.47 years SD = ±7.45 years, minimum–maximum age = 65–95) have been evaluated for their frailty status using different tools. We observed that the PRISMA 7 translation detects frailty only when one question is removed at a cut-off of ≥2 and indicates a sensitivity of 88.1% and specificity of 99.9% with a good correlation with SPPB measurements (r = −0.858; p < 0.001). When CFS was validated using SPPB, it demonstrated a very good correlation (r = −0.838; p < 0.001 respectively) as was the case for the modified Fried Criteria (r = −0.725; p < 0.001). All items demonstrated a good correlation between them. We here propose that we can accurately assess frailty status in the community setting by using a modified version of Fried criteria, Clinical Frailty Scale translation in Greek, and we can screen for frailty by using the Greek translation of PRISMA 7 only after removing item 6 of the questionnaire.
The Stone Skeleton: A Reappraisal
Authors: Boothby Thomas E. , Coronelli Dario
Published: May 2024

Jacques Heyman’s 1966 article “The Stone Skeleton” has been influential in the assessment of masonry arches in general, including masonry bridges and gothic architecture. Heyman’s article relies on limiting assumptions about arch behavior, and concludes that an arch can be declared stable based on the location of a statically admissible thrust line within the arch. In the following commentary, we assess the validity of the assumptions made by Heyman and the uses that have been made of his application of the lower bound theorem of plasticity. We conclude that Heyman’s methods have enduring value, but that the user needs to recognize the limitations of the assumptions made and the limits on the validity of conclusions imposed by these assumptions.
Recent Applications of Unilateral NMR to Objects of Cultural Heritage
Authors: Di Tullio Valeria , Proietti Noemi
Published: May 2024

Although nuclear magnetic resonance (NMR) is recognized as a powerful tool in many areas of research, among the investigative techniques used in the field of cultural heritage its application is still largely unknown. One of the reasons for this is that artifacts are complex heterogeneous systems whose analysis requires a multi-disciplinary approach. In addition, major drawbacks in the analysis of objects belonging to cultural heritage are their limited quantity, number of samples collected from the artifact, and their immovability. Consequently, a methodological approach where non-destructive, and possibly non-invasive techniques are used, is advisable. In recent years, thanks to the development of portable instruments, there has been an increasing use of the NMR methodology in the cultural heritage field. The use of portable NMR has allowed us to study several materials in the cultural heritage, such as frescoes, stones, wood, paper, and paintings, to address the challenges in monitoring dampness in historical masonries, to evaluate the penetration depth of a hydrophobic treatment into a porous material, and to study of the effect of cleaning procedures on artifacts. In this paper, recent studies illustrating the potential of NMR portable methodologies in this field of research are reported.
New Perceptions of Ancient Commerce Driven by Underwater Ancient Site Investigations: A Case Study of Xinfeng River Basin
Authors: Zhang Song , He Ming , Dong Guoliang , Wang Xianying
Published: May 2024

In the 1950s and 1960s, to address the flooding issues and power shortage that hindered national construction, the Xinfeng River hydropower plant was planned and built to prevent floods, store water, and generate electricity. Consequently, many ancient ruins in the study area were drowned, including ancient post roads, channels, villages, towns, bridges, and other relic sites. By checking historical data and adopting integrated underwater acoustic detection, we conducted a comprehensive cultural-relics survey on the flooded area under Wanlv Lake in the Xinfeng River Basin. A side-scan sonar detection of the underwater relics within the flooded area confirmed the spatial distribution of cultural relics in the Xinfeng River Basin. It portrayed ancient people’s production and life scenarios, outlined the migration and trade history within the region and beyond, and contributed to the enrichment of the literature and understanding of ancient shipping and trade in the basin.
Evolving Adult ADHD Care: Preparatory Evaluation of a Prototype Digital Service Model Innovation for ADHD Care
Authors: Patrickson Bronwin , Shams Lida , Fouyaxis John , Strobel Jörg , Schubert Klaus Oliver , Musker Mike , Bidargaddi Niranjan
Published: May 2024

Background: Given the prevalence of ADHD and the gaps in ADHD care in Australia, this study investigates the critical barriers and driving forces for innovation. It does so by conducting a preparatory evaluation of an ADHD prototype digital service innovation designed to help streamline ADHD care and empower individual self-management. Methods: Semi-structured interviews with ADHD care consumers/participants and practitioners explored their experiences and provided feedback on a mobile self-monitoring app and related service innovations. Interview transcripts were double coded to explore thematic barriers and the enablers for better ADHD care. Results: Fifteen interviews (9 consumers, 6 practitioners) revealed barriers to better ADHD care for consumers (ignorance and prejudice, trust, impatience) and for practitioners (complexity, sustainability). Enablers for consumers included validation/empowerment, privacy, and security frameworks, tailoring, and access. Practitioners highlighted the value of transparency, privacy and security frameworks, streamlined content, connected care between services, and the tailoring of broader metrics. Conclusions: A consumer-centred approach to digital health service innovation, featuring streamlined, private, and secure solutions with enhanced mobile tools proves instrumental in bridging gaps in ADHD care in Australia. These innovations should help to address the gaps in ADHD care in Australia. These innovations should encompass integrated care, targeted treatment outcome data, and additional lifestyle support, whilst recognising the tensions between customised functionalities and streamlined displays.
Mitochondria-Derived Vesicles, Sterile Inflammation, and Pyroptosis in Liver Cancer: Partners in Crime or Innocent Bystanders?
Authors: Guerra Flora , Ponziani Francesca Romana , Cardone Ferdinando , Bucci Cecilia , Marzetti Emanuele , Picca Anna
Published: May 2024

Alterations in cellular signaling, chronic inflammation, and tissue remodeling contribute to hepatocellular carcinoma (HCC) development. The release of damage-associated molecular patterns (DAMPs) upon tissue injury and the ensuing sterile inflammation have also been attributed a role in HCC pathogenesis. Cargoes of extracellular vesicles (EVs) and/or EVs themselves have been listed among circulating DAMPs but only partially investigated in HCC. Mitochondria-derived vesicles (MDVs), a subpopulation of EVs, are another missing link in the comprehension of the molecular mechanisms underlying the onset and progression of HCC biology. EVs have been involved in HCC growth, dissemination, angiogenesis, and immunosurveillance escape. The contribution of MDVs to these processes is presently unclear. Pyroptosis triggers systemic inflammation through caspase-dependent apoptotic cell death and is implicated in tumor immunity. The analysis of this process, together with MDV characterization, may help capture the relationship among HCC development, mitochondrial quality control, and inflammation. The combination of immune checkpoint inhibitors (i.e., atezolizumab and bevacizumab) has been approved as a synergistic first-line systemic treatment for unresectable or advanced HCC. The lack of biomarkers that may allow prediction of treatment response and, therefore, patient selection, is a major unmet need. Herein, we overview the molecular mechanisms linking mitochondrial dysfunction, inflammation, and pyroptosis, and discuss how immunotherapy targets, at least partly, these routes.
Multi-System-Level Analysis with RNA-Seq on Pterygium Inflammation Discovers Association between Inflammatory Responses, Oxidative Stress, and Oxidative Phosphorylation
Authors: Kim Ye-Ah , Choi Yueun , Kim Tae Gi , Jeong Jisu , Yu Sanghyeon , Kim Taeyoon , Sheen Kisung , Lee Yoonsung , Choi Taesoo , Park Yong Hwan , Kang Min Seok , Kim Man S.
Published: May 2024

A pterygium is a common conjunctival degeneration and inflammatory condition. It grows onto the corneal surface or limbus, causing blurred vision and cosmetic issues. Ultraviolet is a well-known risk factor for the development of a pterygium, although its pathogenesis remains unclear, with only limited understanding of its hereditary basis. In this study, we collected RNA-seq from both pterygial tissues and conjunctival tissues (as controls) from six patients (a total of twelve biological samples) and retrieved publicly available data, including eight pterygium samples and eight controls. We investigated the intrinsic gene regulatory mechanisms closely linked to the inflammatory reactions of pterygiums and compared Asian (Korea) and the European (Germany) pterygiums using multiple analysis approaches from different perspectives. The increased expression of antioxidant genes in response to oxidative stress and DNA damage implies an association between these factors and pterygium development. Also, our comparative analysis revealed both similarities and differences between Asian and European pterygiums. The decrease in gene expressions involved in the three primary inflammatory signaling pathways—JAK/STAT, MAPK, and NF-kappa B signaling—suggests a connection between pathway dysfunction and pterygium development. We also observed relatively higher activity of autophagy and antioxidants in the Asian group, while the European group exhibited more pronounced stress responses against oxidative stress. These differences could potentially be necessitated by energy-associated pathways, specifically oxidative phosphorylation.
Comparative Transcriptomic Analysis of WSSV-Challenged Penaeus vannamei with Variable Resistance Levels
Authors: Li Xupeng , Xue Qian , Luan Sheng , Luo Kun , Kong Jie , Meng Xianhong
Published: May 2024

The Pacific white shrimp, Penaeus vannamei, is highly susceptible to white spot syndrome virus (WSSV). Our study explored the transcriptomic responses of P. vannamei from resistant and susceptible families, uncovering distinct expression patterns after WSSV infection. The analysis revealed a higher number of differentially expressed genes (DEGs) in the susceptible family following WSSV infection compared to the resistant family, when both were evaluated against their respective control groups, indicating that the host resistance of the family line influences the transcriptome. The results also showed that subsequent to an identical duration following WSSV infection, there were more DEGs in P. vannamei with a high viral load than in those with a low viral load. To identify common transcriptomic responses, we profiled DEGs across families at 96 and 228 h post-infection (hpi). The analysis yielded 64 up-regulated and 37 down-regulated DEGs at 96 hpi, with 33 up-regulated and 34 down-regulated DEGs at 228 hpi, showcasing the dynamics of the transcriptomic response over time. Real-time RT-PCR assays confirmed significant DEG expression changes post-infection. Our results offer new insights into shrimp’s molecular defense mechanisms against WSSV.
BAP31 Plays an Essential Role in Mouse B Cell Development via Regulation of BCR Signaling
Authors: Zhao Bo , An Fei , Hao Zhenzhen , Zhang Wanting , Wang Bing
Published: May 2024

B cell receptor-associated protein 31 (BAP31) is a transmembrane protein that is widely expressed and primarily located in the endoplasmic reticulum (ER). B cells play a crucial role in the immune system, and BAP31 significantly contributes to the functions of various immune cells. However, the specific role of BAP31 in B lymphocytes development remains unknown. In this study, we utilized a mouse model with BAP31 deleted from B cells to investigate its effects. Our findings reveal a block in early B cell development in the bone marrow and a significant decrease in the number of B cells in peripheral lymphoid organs taken from BAP31 B cell conditional knockout (BAP31-BCKO) mice. B cell receptor (BCR) signaling is crucial for the normal development and differentiation of B lymphocytes. BAP31, an endoplasmic reticulum membrane protein, directly regulates the BCR signaling pathway and was shown to be significantly positively correlated with B cell activation and proliferation. These findings establish BAP31 as a crucial regulator of early B cell development.
A Fruit-Expressed MYB Transcription Factor Regulates Anthocyanin Biosynthesis in Atropa belladonna
Authors: Liu Xiaoqiang , Zhao Tengfei , Yuan Lina , Qiu Fei , Tang Yueli , Li Dan , Zhang Fangyuan , Zeng Lingjiang , Yang Chunxian , Nagdy Mohammad Mahmoud , Htun Zun Lai Lai , Lan Xiaozhong , Chen Min , Liao Zhihua , Li Yan
Published: May 2024

Anthocyanins are water-soluble flavonoid pigments that play a crucial role in plant growth and metabolism. They serve as attractants for animals by providing plants with red, blue, and purple pigments, facilitating pollination and seed dispersal. The fruits of solanaceous plants, tomato (Solanum lycopersicum) and eggplant (Solanum melongena), primarily accumulate anthocyanins in the fruit peels, while the ripe fruits of Atropa belladonna (Ab) have a dark purple flesh due to anthocyanin accumulation. In this study, an R2R3-MYB transcription factor (TF), AbMYB1, was identified through association analysis of gene expression and anthocyanin accumulation in different tissues of A. belladonna. Its role in regulating anthocyanin biosynthesis was investigated through gene overexpression and RNA interference (RNAi). Overexpression of AbMYB1 significantly enhanced the expression of anthocyanin biosynthesis genes, such as AbF3H, AbF3′5′H, AbDFR, AbANS, and Ab3GT, leading to increased anthocyanin production. Conversely, RNAi-mediated suppression of AbMYB1 resulted in decreased expression of most anthocyanin biosynthesis genes, as well as reduced anthocyanin contents in A. belladonna. Overall, AbMYB1 was identified as a fruit-expressed R2R3-MYB TF that positively regulated anthocyanin biosynthesis in A. belladonna. This study provides valuable insights into the regulation of anthocyanin biosynthesis in Solanaceae plants, laying the foundation for understanding anthocyanin accumulation especially in the whole fruits of solanaceous plants.
A Systematic Review of Semaglutide’s Influence on Cognitive Function in Preclinical Animal Models and Cell-Line Studies
Authors: Tipa Raluca Oana , Balan Daniela-Gabriela , Georgescu Mihai-Teodor , Ignat Luciana Angela , Vacaroiu Ileana Adela , Georgescu Dragos Eugen , Raducu Laura , Mihai Doina Andrada , Chiperi Liviu-Vasile , Balcangiu-Stroescu Andra-Elena
Published: May 2024

Since we aim to test new options to find medication for cognitive disorders, we have begun to assess the effect of semaglutide and to conduct a review gathering studies that have attempted this purpose. This systematic review focuses on the cognitive effects of semaglutide, a glucagon-like peptide 1 receptor agonist (GLP-1 RA), in the context of neurological and cognitive impairment. Semaglutide, a synthetic GLP-1 analog, showcased neuroprotective effects beyond metabolic regulation. It mitigated apoptosis and improved cognitive dysfunction in cerebrovascular disease, suggesting broader implications for neurological well-being. Also, studies highlighted GLP-1 RAs’ positive impact on olfactory function in obese individuals with type 2 diabetes, on neurodegenerative disorders, multiple sclerosis, and endotoxemia. In order to analyze current studies that assess the impact of semaglutide on cognitive function, a literature search was conducted up to February 2024 on two online databases, MEDLINE (via PubMed) and Web of Science Core Collection, as well as various websites. Fifteen studies on mice populations and two studies on cell lines were included, analyzed, and assessed with bias-specific tools. The neuroprotective and anti-apoptotic properties of GLP-1 and its analogs were emphasized, with animal models and cell line studies demonstrating enhanced cognitive function. While promising, limitations include fewer studies, highlighting the need for extensive research, particularly in the human population. Even though this medication seems promising, there are significant limitations, one of which is the lack of studies on human subjects. Therefore, this review aims to gather current evidence.
The Role of One-Carbon Metabolism and Methyl Donors in Medically Assisted Reproduction: A Narrative Review of the Literature
Authors: Sfakianoudis Konstantinos , Zikopoulos Athanasios , Grigoriadis Sokratis , Seretis Nikolaos , Maziotis Evangelos , Anifandis George , Xystra Paraskevi , Kostoulas Charilaos , Giougli Urania , Pantos Konstantinos , Simopoulou Mara , Georgiou Ioannis
Published: May 2024

One-carbon (1-C) metabolic deficiency impairs homeostasis, driving disease development, including infertility. It is of importance to summarize the current evidence regarding the clinical utility of 1-C metabolism-related biomolecules and methyl donors, namely, folate, betaine, choline, vitamin B12, homocysteine (Hcy), and zinc, as potential biomarkers, dietary supplements, and culture media supplements in the context of medically assisted reproduction (MAR). A narrative review of the literature was conducted in the PubMed/Medline database. Diet, ageing, and the endocrine milieu of individuals affect both 1-C metabolism and fertility status. In vitro fertilization (IVF) techniques, and culture conditions in particular, have a direct impact on 1-C metabolic activity in gametes and embryos. Critical analysis indicated that zinc supplementation in cryopreservation media may be a promising approach to reducing oxidative damage, while female serum homocysteine levels may be employed as a possible biomarker for predicting IVF outcomes. Nonetheless, the level of evidence is low, and future studies are needed to verify these data. One-carbon metabolism-related processes, including redox defense and epigenetic regulation, may be compromised in IVF-derived embryos. The study of 1-C metabolism may lead the way towards improving MAR efficiency and safety and ensuring the lifelong health of MAR infants.
A Nanorobotics-Based Approach of Breast Cancer in the Nanotechnology Era
Authors: Neagu Anca-Narcisa , Jayaweera Taniya , Weraduwage Krishan , Darie Costel C.
Published: May 2024

We are living in an era of advanced nanoscience and nanotechnology. Numerous nanomaterials, culminating in nanorobots, have demonstrated ingenious applications in biomedicine, including breast cancer (BC) nano-theranostics. To solve the complicated problem of BC heterogeneity, non-targeted drug distribution, invasive diagnostics or surgery, resistance to classic onco-therapies and real-time monitoring of tumors, nanorobots are designed to perform multiple tasks at a small scale, even at the organelles or molecular level. Over the last few years, most nanorobots have been bioengineered as biomimetic and biocompatible nano(bio)structures, resembling different organisms and cells, such as urchin, spider, octopus, fish, spermatozoon, flagellar bacterium or helicoidal cyanobacterium. In this review, readers will be able to deepen their knowledge of the structure, behavior and role of several types of nanorobots, among other nanomaterials, in BC theranostics. We summarized here the characteristics of many functionalized nanodevices designed to counteract the main neoplastic hallmark features of BC, from sustaining proliferation and evading anti-growth signaling and resisting programmed cell death to inducing angiogenesis, activating invasion and metastasis, preventing genomic instability, avoiding immune destruction and deregulating autophagy. Most of these nanorobots function as targeted and self-propelled smart nano-carriers or nano-drug delivery systems (nano-DDSs), enhancing the efficiency and safety of chemo-, radio- or photodynamic therapy, or the current imagistic techniques used in BC diagnosis. Most of these nanorobots have been tested in vitro, using various BC cell lines, as well as in vivo, mainly based on mice models. We are still waiting for nanorobots that are low-cost, as well as for a wider transition of these favorable effects from laboratory to clinical practice.
Synthesis, Spectroscopic Characterization, and Photophysical Studies of Heteroleptic Silver Complexes Bearing 2,9-Bis(styryl)-1,10-phenanthroline Ligands and Bis[(2-diphenylphosphino)phenyl] Ether
Authors: Glykos Dimitrios , Tsipis Athanassios C. , Plakatouras John C. , Malandrinos Gerasimos
Published: May 2024

Three new heteroleptic Ag(I) complexes, labeled as [AgL(POP)]BF4 (1–3), were successfully synthesized and comprehensively characterized. Here, L represents 2,9-bis((E)-4-methoxystyryl)-1,10-phenanthroline (L1), 2,9-bis((E)-4-methylthiostyryl) -1,10-phenanthroline (L2), and 2,9-bis((E)-4-diethylaminostyryl)-1,10-phenanthroline (L3), while POP stands for Bis[(2-diphenylphosphino)phenyl] ether. The stability of these compounds in solution was confirmed through multinuclear 1D (1H, 13C, 31P) and 2D NMR (COSY, NOESY, HMBC, HSQC) spectroscopies. Additionally, their molecular structure was elucidated via X-ray crystallography. The photophysical properties of the complexes were assessed both in the solid state and in solution (dichloromethane). Compounds 1–3 demonstrated moderate emissions in solution, with quantum yields ranging from 11–23%. Interestingly, their solid-state luminescent behavior differed. Large bathochromic shifts (42–75 nm) of the emission maxima and a decrease in quantum yields (2.5–9.5%) were evident, possibly due to the presence of excimers. Compound 3 stands out as a rare example of an Ag(I) red-color emitter.
Fluorosolvatochromism of Platinum Supramolecular Coordination Complexes: Stepwise Synthesis and Photophysical Properties of Organometallic Tetranuclear Pt(II) Squares
Authors: Garypidou Antonia , Ypsilantis Konstantinos , Garoufis Achilleas
Published: May 2024

The stepwise synthesis and characterization of three new mixed-ligand organometallic tetranuclear platinum squares were achieved. All of the complexes were constituted by the conjunction of two (2,2′-bpy)Pt-terph-Pt(2,2′-bpy) (terph = p-terphenyl) fragments linked by a variety of N^N ligands (4,4′-bipyridine (4,4′-bpy), 1,4-di(pyridin-4-yl)benzene (dpbz), and 4,4′-di(pyridin-4-yl)-1,1′-biphenyl (dpbph)), which occupied the fourth coordination site of each metal center, giving rise to square-shaped molecules of the general formula [Pt2(2,2′-bpy)2(terph)(N^N)]2. Consequently, the tetranuclear complexes, {[Pt(2,2′-bpy)]4(μ-terph)2(μ-4,4′-bpy)2}{PF6}4 (7), {[Pt(2,2′-bpy)]4(μ-terph)2(μ-dpbz)2}{PF6}4 (8), and {[Pt(2,2′-bpy)]4(μ-terph)2(μ-dpbph)2}{PF6}4 (9) were constructed. The photophysical properties of these complexes were studied both in the solid state and in various solvents, revealing fluorosolvatochromism.
Electrothermal Instabilities in Barium-Titanate-Based Ceramics
Authors: Krikkis Rizos N.
Published: May 2024

An electrothermal analysis for barium-titanate-based ceramics is presented, combining the Heywang–Jonker model for the electric resistivity with a heat dissipation mechanism based on natural convection and radiation in a one-dimensional model on the device level with voltage as the control parameter. Both positive-temperature-coefficient (PTC) and negative temperature coefficient (NTC) effects are accounted for through the double Schottky barriers at the grain boundaries of the material. The problem formulated in this way admits uniform and non-uniform multiple-steady-state solutions that do not depend on the external circuit. The numerical bifurcation analysis reveals that the PTC effect gives rise to several multiplicites above the Curie point, whereas the NTC effect is responsible for the thermal runaway (temperature blowup). The thermal runaway phenomenon as a potential thermal shock could be among the possible reasons for the observed thermomechanical failures (delamination fracture). The theoretical results for the NTC regime and the thermal runaway are in agreement with the experimental flash sintering results obtained for barium titanate, and 3% and 8% yttria-stabilized zirconia.
Is the Early Screening of Lower Genital Tract Infections Useful in Preventing Adverse Obstetrical Outcomes in Twin Pregnancy?
Authors: Roero Sofia , Benedetto Giulia , Charrier Lorena , Ingala Agata , Ronco Alice , Fea Teresa , Borgarello Valentina , Bossotti Carlotta , Arduino Silvana , Revelli Alberto
Published: May 2024

Objectives: Twin pregnancy implies a higher risk of preterm birth and, consequently, higher neonatal morbidity and mortality. In singleton pregnancies, infections of the lower genital tract (LGTIs) and bacterial vaginosis are associated with preterm labor, and their early detection has been proven effective in reducing complications like the preterm premature rupture of membranes (pPROM) and preterm delivery. The same evidence, however, is lacking for twin pregnancies. This study aimed to evaluate whether the early identification and treatment of LGTIs or bacterial vaginosis in asymptomatic women with twin pregnancy could reduce the rate of miscarriages, pPROM, and preterm birth. Methods: This study performed a retrospective comparison of 285 women with a multiple pregnancy submitted for a cervico-vaginal swab only at 20–22 weeks (Single Test Group, STG), and 199 women who underwent the swab at 12–14 and again at 20–22 weeks (Double Test Group, DTG). All women included in the study had a twin pregnancy and were followed up at Sant’Anna Hospital, Turin (Italy), between September 2012 and February 2021. Results: In STG, 21.7% of patients had a positive swab; in DTG, 19.9% had an early positive swab that was immediately treated by targeted antibiotics; and 16.7% had a mid-pregnancy positive swab. The DTG showed a significantly lower incidence of pPROM in univariate analysis (14.4% vs. 23.1%, p = 0.021), which was confirmed by multivariate analysis (OR 0.55, CI 0.33–0.93, p = 0.025). Conclusions: Our study suggests that, in asymptomatic women with twin pregnancy, the early screening of LGTIs and bacterial vaginosis by a cervico-vaginal swab at 12–14 weeks of gestational age is effective in reducing the risk of pPROM.
Repaired Tetralogy of Fallot: Have We Understood the Right Timing of PVR?
Authors: Leonardi Benedetta , Perrone Marco , Calcaterra Giuseppe , Sabatino Jolanda , Leo Isabella , Aversani Martina , Bassareo Pier Paolo , Pozza Alice , Oreto Lilia , Moscatelli Sara , Borrelli Nunzia , Bianco Francesco , Di Salvo Giovanni
Published: May 2024

Despite many advances in surgical repair during the past few decades, the majority of tetralogy of Fallot patients continue to experience residual hemodynamic and electrophysiological abnormalities. The actual issue, which has yet to be solved, is understanding how this disease evolves in each individual patient and, as a result, who is truly at risk of sudden death, as well as the proper timing of pulmonary valve replacement (PVR). Our responsibility should be to select the most appropriate time for each patient, going above and beyond imaging criteria used up to now to make such a clinically crucial decision. Despite several studies on timing, indications, procedures, and outcomes of PVR, there is still much uncertainty about whether PVR reduces arrhythmia burden or improves survival in these patients and how to appropriately manage this population. This review summarizes the most recent research on the evolution of repaired tetralogy of Fallot (from adolescence onwards) and risk factor variables that may favor or delay PVR.