bluuebunny/crossref_metadata_2025 huggingface dataset:
A Nationwide Virtual Research Education Program for Medical Students in Pakistan: Methodological Framework, Feasibility Testing, and Outcomes
Authors: Noorali Ali Aahil , Inam Maha , Shahbaz Hamna , Rauf Hareem , Aamir Faiqa Binte , Khalid Farah , Abbas Saadia , Saeed Abdullah , Musharraf Muhammad Daniyal , Merchant Asma Altaf Hussain , Hasan Babar S. , Rasheed Muneera A. , Jehan Fyezah , Tariq Muhammad , Haider Adil Hussain
Published: January 2022

Introduction: Equipping young medical trainees with fundamental research skills can be a promising strategy to address the need for professionals who can understand and responsibly communicate evolving scientific evidence during a pandemic. Despite an ardent interest to partake in research, most educational institutions in Pakistan and other low-middle income countries have not yet adopted a comprehensive strategy for research skills education. The authors aimed to design and assess the feasibility of implementing the first nation-wide virtual research workshop for medical students in Pakistan.

Methods: The course “Beginners Guide to Research,” designed as a nation-wide virtual research workshop series, was conducted for medical students across Pakistan in June 2020. Four interactive live workshops took place online on alternate days from June 22nd, 2020, to June 27th, 2020, each lasting 1–2 h. Outcomes included: (i) reach, (ii) efficacy as indexed by pre-post change in score pertaining to knowledge and application of research and (iii) self-rated perceptions about understanding of research on a Likert scale.

Results: 3,862 participants enrolled from 41 cities and 123 institutions. Enrolled participants belonged to the following provinces: Sindh (n = 1,852, 48.0%), Punjab (n = 1,767, 45.8%), Khyber Pakhtunkhwa (n = 109, 2.8%), Azad Jammu and Kashmir (n = 84, 2.2%) Balochistan (n = 42, 1.1%). We also saw a few registrations from international students (n = 8, 0.2%). Mean (SD) age of enrolled medical students was 21.1 (2.1) years, 2,453 (63.5%) participants were female and 2,394 (62.0%) were from private-sector medical colleges. Two thousand ninety-three participants participants filled out all four pre-test and post-test forms. The total median knowledge score improved from 39.7 to 60.3% with the highest improvements in concepts of research bioethics and literature search (p < 0.001) with greater change for females compared to males (+20.6 vs. +16.2%, p < 0.001) and private institutions compared to public ones (+16.2 vs. +22.1%, p < 0.001).

Conclusion: The overwhelming enrollment and significant improvement in learning outcomes (>50% of baseline) indicate feasibility of a medical student-led research course during a pandemic, highlighting its role in catering to the research needs in the LMICs.
Characteristics of Patients With Trigeminal Neuralgia Referred to the Indonesian National Brain Center Neurosurgery Clinic
Authors: Prasetya Mustaqim , Adidharma Peter , Sulistyanto Adi , Inoue Takuro , Arham Abrar
Published: January 2022

Trigeminal neuralgia (TN) is a debilitating neuropathic pain involving the fifth cranial nerve. There has been no study investigating the clinical and socioeconomical characteristics of patients with TN in Indonesia. A total of 100 patients were included in this study. Symptoms indicating a later stage of the illness, namely, involvement of all the trigeminal nerve branches, numbness, and concomitant persistent pain, were the common presentations found in our cohort. Only one TN diagnosis was made by a general practitioner (GP). None were immediately referred to a neurosurgeon following their diagnosis. Access to our clinic took as long as 4.7 ± 5.1 years (mean ± SD) from the onset. Older age was a significant predictor of an increased likelihood of not knowing their illness upon the referral (21.9%, p = 0.008). Upon their first presentation, 25.5% of patients had experienced drug-related side effects due to prolonged medication. Only 50% of patients were compensated by the universal health coverage (UHC) system. Seven patients spent ≥ 50 million rupiahs and eight patients had already lost their jobs. In conclusion, early contact with a neurosurgeon contributes to better management of TN, both for the patients and healthcare system in Indonesia. A refined understanding of TN nature is still needed in this country.
Single-Use vs. Reusable Digital Flexible Ureteroscope to Treat Upper Urinary Calculi: A Propensity-Score Matching Analysis
Authors: Huang Fang , Zhang Xiaoqiong , Cui Yu , Zhu Zewu , Li Yongchao , Chen Jinbo , Zeng Feng , Li Yang , Chen Zhiyong , Chen Hequn
Published: January 2022

Objective: The purpose of this research was to compare the treatment outcomes and costs of a single-use and reusable digital flexible ureteroscope for upper urinary calculi.

Methods: Four hundred forty patients with reusable digital flexible ureteroscope and 151 patients with single-use flexible digital ureteroscope were included in this study. Through exclusion and inclusion criteria and 1:1 propensity-score matching analysis based on baseline characteristics, ultimately, 238 patients (119:119) were compared in terms of treatment outcomes. The cost analysis was based on the costs of purchase, repair, and reprocessing divided by the number of all procedures in each group (450 procedures with reusable digital flexible ureteroscope and 160 procedures with single-use digital flexible ureteroscope).

Results: There was no statistical significance in mean operation time (P = 0.666). The single-use digital flexible ureteroscope group has a shorter mean length of hospital stay than the reusable digital flexible ureteroscope group (P = 0.026). And the two groups have a similar incidence of postoperative complications (P = 0.678). No significant difference was observed in the final stone-free rate (P = 0.599) and the probability of secondary lithotripsy (P = 0.811) between the two groups. After 275 procedures, the total costs of a single-use flexible ureteroscope would exceed the reusable flexible ureteroscope.

Conclusion: Our data demonstrated that the single-use digital flexible ureteroscope is an alternative to reusable digital flexible ureteroscopy in terms of surgical efficacy and safety for upper urinary calculi. In terms of the economics of the two types of equipment, institutions should consider their financial situation, the number of FURS procedures, the volume of the patient's calculus, surgeon experience, and local dealerships' annual maintenance contract when making the choice.
Year-Long Phenotypical Study of Calves Derived From Different Assisted-Reproduction Technologies
Authors: Lopes Jordana S. , Soriano-Úbeda Cristina , París-Oller Evelyne , Navarro-Serna Sergio , Canha-Gouveia Analuce , Sarrias-Gil Lucía , Cerón José Joaquin , Coy Pilar
Published: January 2022

Assisted reproductive technologies play a major role in the cattle industry. An increase in the use of in vitro-derived embryos is currently being seen around the globe. But the efficiency and quality of the in vitro-derived embryos are substandard when compared to the in vivo production. Different protocols have been designed to overcome this issue, one of those being the use of reproductive fluids as supplementation to embryo culture media. In this study, in vitro-derived calves produced with reproductive fluids added to their embryo production protocol were followed for the first year of life pairwise with their in vivo control, produced by artificial insemination (AI), and their in vitro control, produced with standard supplementation in embryo production. The objective was to assess if any differences could be found in terms of growth and development as well as hematological and biochemical analytes between the different systems. All the analysed variables (physical, hematological, and biochemical) were within physiological range and very similar between calves throughout the entire experiment. However, differences were more evident between calves derived from standard in vitro production and AI. We concluded that the use of reproductive fluids as a supplementation to the embryo culture media results in calves with closer growth and development patterns to those born by AI than the use of bovine serum albumin as supplementation.
Biological Function of Plant Tannin and Its Application in Animal Health
Authors: Tong Zhenkai , He Wenfeng , Fan Xiao , Guo Aiwei
Published: January 2022

Plant tannins are widely found in plants and can be divided into hydrolyzed tannins and condensed tannins. In recent years, researchers have become more and more interested in using tannin-rich plants and plant extracts in ruminant diets to improve the quality of animal products. Some research results show that plant tannins can effectively improve the quality of meat and milk, and enhance the oxidative stability of the product. In this paper, the classification and extraction sources of plant tannins are reviewed, as well as the biological functions of plant tannins in animals. The antioxidant function of plant tannins is discussed, and the influence of their structure on antioxidation is analyzed. The effects of plant tannins against pathogenic bacteria and the mechanism of action are discussed, and the relationship between antibacterial action and antioxidant action is analyzed. The inhibitory effect of plant tannins on many kinds of pathogenic viruses and their action pathways are discussed, as are the antiparasitic properties of plant tannins. The anti-inflammatory action of tannins and its mechanism are analyzed. The function of plant tannins in antidiarrheal action and its influencing factors are discussed. In addition, the effects of plant tannins as feed additives on animals and the influencing factors are reviewed in this paper to provide a reference for further research.
Trust in Institutions and the COVID-19 Threat: A Cross-Sectional Study on the Public Perception of Official Recommendations and of Othering in Switzerland
Authors: Gilles Ingrid , Le Pogam Marie-Annick , Perriraz Margaux , Bangerter Adrian , Green Eva G. T. , Staerklé Christian , Krings Franciska , Wagner-Egger Pascal , Peytremann-Bridevaux Isabelle
Published: January 2022

Objectives: To explore how perceived disease threat and trust in institutions relate to vaccination intent, perceived effectiveness of official recommendations, and to othering strategies.

Methods: We conducted a cross-sectional survey of Swiss adults in July 2020. Outcome variables were vaccination intent, perceived effectiveness of official recommendations and othering strategies (labelling a given social group as responsible for the disease and distancing from it). Independent variables were perceived disease threat, trust in various institutions, perceived health-related measures, and sociodemographic variables. Linear and logistic regressions were performed.

Results: The response rate was 20.2% (1518/7500). Perceived disease threat and trust in medical/scientific institutions were positively associated with vaccination intent and perceived effectiveness of official recommendations for coronavirus mitigation measures. Only disease threat was associated with a perception of effectiveness among othering strategies. Age and education levels were associated with vaccination intent.

Conclusion: Reinforcing trust in medical/scientific institutions can help strengthen the perceived effectiveness of official recommendations and vaccination. It however does not prevent adherence to ineffective protecting measures such as othering strategies, where decreasing perceptions of epidemic threat appears to be more efficient.
An Inventory of Deceased Donor Family Care and Contact Between Donor Families and Recipients in 15 European Countries
Authors: Wind Tineke , Jansen Nichon , Flodén Anne , Haase-Kromwijk Bernadette , Shaw David , Gardiner Dale
Published: January 2022

Families of organ donors play an important role in the deceased organ donation process. The aim of this study was to gain insight into donor family care by creating an inventory of practice in various European countries. A questionnaire about donor family care and contact between donor families and recipients was developed. Representatives of the organ donor professionals of 15 European countries responded (94%). The donor coordinator plays a key role in care for the donor family. All countries provide information about the donation results to the families, although diminished due to privacy laws. Anonymous written contact between donor families and recipients is possible in almost all countries and direct contact in only a few. Remembrance ceremonies exist in most countries. Half of the respondents thought the aftercare could improve. This first inventory shows that differences exist between countries, depending on the organisation of the donation process, the law and the different role of the professionals. Direct contact between donor families and recipients is rarely supported by the donation organisation. To date there has been limited research about the experience of donor family aftercare and we would urge all donation organisations to consider this as a priority area.
Detection and Recognition of Drones Based on a Deep Convolutional Neural Network Using Visible Imagery
Authors: Samadzadegan Farhad , Dadrass Javan Farzaneh , Ashtari Mahini Farnaz , Gholamshahi Mehrnaz
Published: January 2022

Drones are becoming increasingly popular not only for recreational purposes but also in a variety of applications in engineering, disaster management, logistics, securing airports, and others. In addition to their useful applications, an alarming concern regarding physical infrastructure security, safety, and surveillance at airports has arisen due to the potential of their use in malicious activities. In recent years, there have been many reports of the unauthorized use of various types of drones at airports and the disruption of airline operations. To address this problem, this study proposes a novel deep learning-based method for the efficient detection and recognition of two types of drones and birds. Evaluation of the proposed approach with the prepared image dataset demonstrates better efficiency compared to existing detection systems in the literature. Furthermore, drones are often confused with birds because of their physical and behavioral similarity. The proposed method is not only able to detect the presence or absence of drones in an area but also to recognize and distinguish between two types of drones, as well as distinguish them from birds. The dataset used in this work to train the network consists of 10,000 visible images containing two types of drones as multirotors, helicopters, and also birds. The proposed deep learning method can directly detect and recognize two types of drones and distinguish them from birds with an accuracy of 83%, mAP of 84%, and IoU of 81%. The values of average recall, average accuracy, and average F1-score were also reported as 84%, 83%, and 83%, respectively, in three classes.
Effectiveness of Three Terminating Products on Reducing the Residual Moisture in Dwarf Castor Plants: A Preliminary Study of Direct Mechanical Harvesting in Central Greece
Authors: Latterini Francesco , Stefanoni Walter , Cavalaris Chris , Karamoutis Christos , Pari Luigi , Alexopoulou Efthymia
Published: January 2022

The contribution of castor oil for reaching the targets set by RED1 and RED2 in Europe can be tangible if the problem related to the mechanical harvesting is overcome. Dwarf hybrids suitable for mechanical harvesting are already available on the market but the residual moisture of plants and capsules has to be lowered in order to allow mechanization. In the present case of study, three common terminating products (Glyphosate GLY, Diquat DIQ and Spotlight DEF) were tested on Kaiima C1012 hybrid in a complete randomized block design to assess the effectiveness of using chemical products to decrease residual moisture in castor plants. Plants were harvested via combine harvester equipped with cereal header to evaluate seed loss (due to dehiscence, impact and cleaning shoe) and the dehulling capacity of the combine harvester’s cleaning shoe. DIQ decreased significantly moisture content of capsules (7.32%) in comparison to the other treatments, while the lowest plant moisture was recorded in DIQ (62.38%) and GLY (59.12%). The use of DIQ triggered the highest impact seed loss (61.75%) in comparison with GLY (46.50%) and DEF (29.02%). Control plants could not be harvested mechanically due to the high residual moisture content and high density of weeds. The present case of study provides highlights regarding the need to further investigate the best practice to terminate castor plants and to develop a specific combine header to reduce seed loss from impact.
Effect of 50 Years of No-Tillage, Stubble Retention, and Nitrogen Fertilization on Soil Respiration, Easily Extractable Glomalin, and Nitrogen Mineralization
Authors: Jha Pramod , Hati Kuntal M. , Dalal Ram C. , Dang Yash P. , Kopittke Peter M. , McKenna Brigid A. , Menzies Neal W.
Published: January 2022

In subtropical regions, we have an incomplete understanding of how long-term tillage, stubble, and nitrogen (N) fertilizer management affects soil biological functioning. We examined a subtropical site managed for 50 years using varying tillage (conventional till (CT) and no-till (NT)), stubble management (stubble burning (SB) and stubble retention (SR)), and N fertilization (0 (N0), 30 (N30), and 90 (N90) kg ha−1 y−1) to assess their impact on soil microbial respiration, easily extractable glomalin-related soil protein (EEGRSP), and N mineralization. A significant three-way tillage × stubble × N fertilizer interaction was observed for soil respiration, with NT+SB+N0 treatments generally releasing the highest amounts of CO2 over the incubation period (1135 mg/kg), and NT+SR+N0 treatments releasing the lowest (528 mg/kg). In contrast, a significant stubble × N interaction was observed for both EEGRSP and N mineralization, with the highest concentrations of both EEGRSP (2.66 ± 0.86 g kg−1) and N mineralization (30.7 mg/kg) observed in SR+N90 treatments. Furthermore, N mineralization was also positively correlated with EEGRSP (R2 = 0.76, p < 0.001), indicating that EEGRSP can potentially be used as an index of soil N availability. Overall, this study has shown that SR and N fertilization have a positive impact on soil biological functioning.
Genome-Wide Association Study of Leaf Chlorophyll Content Using High-Density SNP Array in Peanuts (Arachis hypogaea L.)
Authors: Zou Kunyan , Kim Ki-Seung , Kang Dongwoo , Kim Min-Cheol , Ha Jungmin , Moon Jung-Kyung , Jun Tae-Hwan
Published: January 2022

The content of chlorophyll, a fundamental component required for photosynthesis in plants, has been widely studied across crop species. In this study, we aimed to evaluate the genetic diversity of 453 peanut accessions. We evaluated the evolutionary relationships using a genome-wide association study (GWAS) of leaf color data based on chlorophyll content analysis using the Axiom_Arachis array containing 58K single-nucleotide polymorphisms (SNPs). We identified seven SNPs as being significantly associated with leaf chlorophyll content on the chromosomes Aradu.A02, Aradu.A08, Araip.B02, Araip.B05, Araip.B06, and Araip.B08 in a GAPIT analysis. The SNP AX-176820297 on Araip.B05 was significantly linked with leaf chlorophyll content across the seasons. The Arahy.SDG4EV gene was detected to be in linkage disequilibrium (LD) with the significant SNPs, and its expression was significantly correlated with leaf chlorophyll content. The results of the current study provide useful and fundamental information with which to assess genetic variations in chlorophyll content and can be utilized for further genetic and genomic studies and breeding programs in peanuts.
Differential Effects of Abiotic Factors on the Insecticidal Efficacy of Diatomaceous Earth against Three Major Stored Product Beetle Species
Authors: Baliota Georgia V. , Lampiri Evagelia , Athanassiou Christos G.
Published: January 2022

This study evaluated the influence of temperature and relative humidity (RH) on the insecticidal effect of diatomaceous earth (DE) at two concentrations, 500 and 1000 ppm, on wheat, for the control of Cryptolestes ferrugineus (Stephens) (Coleoptera: Cucujidae), Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae), and Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae). These bioassays were carried out in all combinations of four temperature levels (15, 20, 25, and 30 °C), and two relative humidity levels (55 and 75%). Cryptolestes ferrugineus and O. surinamensis were found to be much more susceptible to the DE-treated wheat than T. confusum, but the increase of the DE dose increased the mortality level for all three species. Although the increase of temperature and the decrease of RH increased insect mortality in some of the combinations tested, the reverse was observed in some treatments, suggesting that there are considerable differential effects of these factors in DE efficacy. The increase of insect exposure from 1 to 21 days notably increased insect mortality, suggesting that exposure is a critical factor that may alleviate possible differential effects of certain abiotic conditions. The results of the present work provide data that illustrate the viability of the utilization of DE in stored product protection, as alternatives to chemical control methods.
Biocontrol Potential and Catabolic Profile of Endophytic Diaporthe eres Strain 1420S from Prunus domestica L. in Poland—A Preliminary Study
Authors: Abramczyk Barbara , Marzec-Grządziel Anna , Grządziel Jarosław , Król Ewa , Gałązka Anna , Oleszek Wiesław
Published: January 2022

Recently, Diaporthe has been considered the most frequently isolated genera of endophytic fungi, having a broad spectrum of host plants and a worldwide distribution. The endophytic Diaporthe strain used in the present work came from the Fungal Collection of Phytopathology and Mycology Subdepartment, University of Life Sciences in Lublin (Poland), and was isolated from healthy Prunus domestica shoots during previous studies. Due to the possibility of using the Diaporthe endophytes as a promising option for plant disease management, the main goal of the research was to study the antagonistic effect of endophytic Diaporthe strain against six phytopathogens: Verticillium dahliae, Botrytis cinerea, Fusarium avenaceum, F. sprotrichioides, Alternaria alternata, and Trichothecium roseum based on the dual culture assay and to determine the catabolic profile of the endophyte by using Biolog FF Plates. The dual-culture test assay revealed the ability of the endophytic Diaporthe to limit the growth of all tested pathogens. The growth inhibition percentage ranged from 20% (V. dahliae) to 40% (T. roseum). A distinct zone of inhibition occurred between the endophytic Diaporthe and the pathogens T. roseum, V. dahliae, and B. cinerea in the co-growth combinations. As for the catabolic profile results, the most intensive utilization of carbon substrates was observed after 168 h of incubation. The growth of the analyzed strain was observed on 79 media containing carbohydrates, carboxylic acids, amino acids, amines and amides, polymers, and others. The most effective decomposition was observed in the polymers group, the least in amines and amides. Molecular identification indicated that this strain was closely related to the Diaporthe eres species complex.
Calibration Method for Line-Structured Light Three-Dimensional Measurements Based on a Single Circular Target
Authors: Wang Jun , Li Xuexing
Published: January 2022

Single circular targets are widely used as calibration objects during line-structured light three-dimensional (3D) measurements because they are versatile and easy to manufacture. This paper proposes a new calibration method for line-structured light 3D measurements based on a single circular target. First, the target is placed in several positions and illuminated by a light beam emitted from a laser projector. A camera captures the resulting images and extracts an elliptic fitting profile of the target and the laser stripe. Second, an elliptical cone equation defined by the elliptic fitting profile and optical center of the camera is established based on the projective geometry. By combining the obtained elliptical cone and the known diameter of the circular target, two possible positions and orientations of the circular target are determined and two groups of 3D intersection points between the light plane and the circular target are identified. Finally, the correct group of 3D intersection points is filtered and the light plane is progressively fitted. The accuracy and effectiveness of the proposed method are verified both theoretically and experimentally. The obtained results indicate that a calibration accuracy of 0.05 mm can be achieved for an 80 mm × 80 mm planar target.
Improvement of Position Repeatability of a Linear Stage with Yaw Minimization
Authors: Cho Doo-Hyun , Kwon Hyo-Chan , Kim Kwon-Hee
Published: January 2022

Recently, due to the miniaturization of electronic products, printed circuit boards (PCBs) have also become smaller. This trend has led to the need for high-precision electrical test equipment to check PCBs for disconnections and short circuits. The purpose of this study is to improve the position repeatability of the platform unit up to ±2.5 μm in linear stage type test equipment. For this purpose, the causes of the position errors of the platform unit are analyzed. The platform unit holding the PCB is driven by a single-axis linear ball screw drive system offset from its geometric center due to design constraints. The yaw rotation of the platform is found to have a dominant effect on position repeatability. To address this problem, adding balancing weights to the platform unit and adjusting the stiffness of the LM Guides are proposed. These methods reduce the yaw rotation by moving the centers of mass and stiffness closer to the linear ball screw actuator. In the verification tests, the position repeatability was decreased to less than ±1.0 μm.
Determining Extreme Still Water Levels for Design and Planning Purposes Incorporating Sea Level Rise: Sydney, Australia
Authors: Watson Phil J.
Published: January 2022

This paper provides an Extreme Value Analysis (EVA) of the hourly water level record at Fort Denison dating back to 1915 to understand the statistical likelihood of the combination of high predicted tides and the more dynamic influences that can drive ocean water levels higher at the coast. The analysis is based on the Peaks-Over-Threshold (POT) method using a fitted Generalised Pareto Distribution (GPD) function to estimate extreme hourly heights above mean sea level. The analysis highlights the impact of the 1974 East Coast Low event and rarity of the associated measured water level above mean sea level at Sydney, with an estimated return period exceeding 1000 years. Extreme hourly predictions are integrated with future projections of sea level rise to provide estimates of relevant still water levels at 2050, 2070 and 2100 for a range of return periods (1 to 1000 years) for use in coastal zone management, design, and sea level rise adaptation planning along the NSW coastline. The analytical procedures described provide a step-by-step guide for practitioners on how to develop similar baseline information from any long tide gauge record and the associated limitations and key sensitivities that must be understood and appreciated in applying EVA.
Biometeorological Conditions during the August 2015 Mega-Heat Wave and the Summer 2010 Mega-Heat Wave in Ukraine
Authors: Shevchenko Olga , Snizhko Sergiy , Zapototskyi Sergii , Matzarakis Andreas
Published: January 2022

The human-biometeorological conditions in Ukraine during two mega-heat waves were analyzed. The evaluation is based on physiologically equivalent temperature (PET). The calculation of PET is performed utilizing the RayMan model. The results revealed these two mega-heat waves produced strenuous human-biometeorological conditions on the territory of Ukraine. During the summer 2010 mega-heat wave, strong and extreme heat stress prevailed at about midday at the stations where this atmospheric phenomenon was observed. The mega-heat wave of August 2015 was characterized by a lower heat load. The diurnal variation of PET values during the researched mega-HW was similar to that of the diurnal variation of air temperature with minimum values in the early morning and maximum values in the afternoon. On the territory where mega-heat waves were observed, the number of days during which heat stress occurred for 9 h amounted to 97.6% for the period from 31 July to 12 August 2010 and 77.1% for the mega-heat wave of August 2015.
Long-Term Changes in Ionospheric Climate in Terms of foF2
Authors: Laštovička Jan
Published: January 2022

There is not only space weather; there is also space climate. Space climate includes the ionospheric climate, which is affected by long-term trends in the ionosphere. One of the most important ionospheric parameters is the critical frequency of the ionospheric F2 layer, foF2, which corresponds to the maximum ionospheric electron density, NmF2. Observational data series of foF2 have been collected at some stations for as long as over 60 years and continents are relatively well covered by a network of ionosondes, instruments that measure, among others, foF2. Trends in foF2 are relatively weak. The main global driver of long-term trends in foF2 is the increasing concentration of greenhouse gases, namely CO2, in the atmosphere. The impact of the other important trend driver, the secular change in the Earth’s main magnetic field, is very regional, being positive in some regions, negative in others, and neither in the rest. There are various sources of uncertainty in foF2 trends. One is the inhomogeneity of long foF2 data series. The main driver of year-to-year changes in foF2 is the quasi-eleven-year solar cycle. The removal of its effect is another source of uncertainty. Different methods might provide somewhat different strengths among trends in foF2. All this is briefly reviewed in the paper.
An Empirical Comparison of Portuguese and Multilingual BERT Models for Auto-Classification of NCM Codes in International Trade
Authors: de Lima Roberta Rodrigues , Fernandes Anita M. R. , Bombasar James Roberto , da Silva Bruno Alves , Crocker Paul , Leithardt Valderi Reis Quietinho
Published: January 2022

Classification problems are common activities in many different domains and supervised learning algorithms have shown great promise in these areas. The classification of goods in international trade in Brazil represents a real challenge due to the complexity involved in assigning the correct category codes to a good, especially considering the tax penalties and legal implications of a misclassification. This work focuses on the training process of a classifier based on bidirectional encoder representations from transformers (BERT) for tax classification of goods with MCN codes which are the official classification system for import and export products in Brazil. In particular, this article presents results from using a specific Portuguese-language-pretrained BERT model, as well as results from using a multilingual-pretrained BERT model. Experimental results show that Portuguese model had a slightly better performance than the multilingual model, achieving an MCC 0.8491, and confirms that the classifiers could be used to improve specialists’ performance in the classification of goods.
Impact of Electrospun Piezoelectric Core–Shell PVDFhfp/PDMS Mesh on Tenogenic and Inflammatory Gene Expression in Human Adipose-Derived Stem Cells: Comparison of Static Cultivation with Uniaxial Cyclic Tensile Stretching
Authors: Baumgartner Walter , Wolint Petra , Hofmann Silvan , Nüesch Cléa , Calcagni Maurizio , Brunelli Marzia , Buschmann Johanna
Published: January 2022

Specific microenvironments can trigger stem cell tenogenic differentiation, such as specific substrates or dynamic cell cultivation. Electrospun meshes composed by core–shell fibers (random or aligned; PDMS core; piezoelectric PVDFhfp shell) were fabricated by coaxial electrospinning. Elastic modulus and residual strain were assessed. Human ASCs were seeded on such scaffolds either under static conditions for 1 week or with subsequent 10% dynamic stretching for 10,800 cycles (1 Hz, 3 h), assessing load elongation curves in a Bose® bioreactor system. Gene expression for tenogenic expression, extracellular matrix, remodeling, pro-fibrotic and inflammatory marker genes were assessed (PCR). For cell-seeded meshes, the E modulus increased from 14 ± 3.8 MPa to 31 ± 17 MPa within 3 h, which was not observed for cell-free meshes. Random fibers resulted in higher tenogenic commitment than aligned fibers. Dynamic cultivation significantly enhanced pro-inflammatory markers. Compared to ASCs in culture flasks, ASCs on random meshes under static cultivation showed a significant upregulation of Mohawk, Tenascin-C and Tenomodulin. The tenogenic commitment expressed by human ASCs in contact with random PVDFhfp/PDMS paves the way for using this novel highly elastic material as an implant to be wrapped around a lacerated tendon, envisioned as a functional anti-adhesion membrane.
CFD-Based and Experimental Hydrodynamic Characterization of the Single-Use Bioreactor XcellerexTM XDR-10
Authors: Kreitmayer Diana , Gopireddy Srikanth R. , Matsuura Tomomi , Aki Yuichi , Katayama Yuta , Nakano Takuya , Eguchi Takuma , Kakihara Hirofumi , Nonaka Koichi , Profitlich Thomas , Urbanetz Nora A. , Gutheil Eva
Published: January 2022

Understanding the hydrodynamic conditions in bioreactors is of utmost importance for the selection of operating conditions during cell culture process development. In the present study, the two-phase flow in the lab-scale single-use bioreactor XcellerexTM XDR-10 is characterized for working volumes from 4.5 L to 10 L, impeller speeds from 40 rpm to 360 rpm, and sparging with two different microporous spargers at rates from 0.02 L min−1 to 0.5 L min−1. The numerical simulations are performed with the one-way coupled Euler–Lagrange and the Euler–Euler models. The results of the agitated liquid height, the mixing time, and the volumetric oxygen mass transfer coefficient are compared to experiments. For the unbaffled XDR-10, strong surface vortex formation is found for the maximum impeller speed. To support the selection of suitable impeller speeds for cell cultivation, the surface vortex formation, the average turbulence energy dissipation rate, the hydrodynamic stress, and the mixing time are analyzed and discussed. Surface vortex formation is observed for the maximum impeller speed. Mixing times are below 30 s across all conditions, and volumetric oxygen mass transfer coefficients of up to 22.1 h−1 are found. The XDR-10 provides hydrodynamic conditions which are well suited for the cultivation of animal cells, despite the unusual design of a single bottom-mounted impeller and an unbaffled cultivation bioreactor.
Sex Differences in Spironolactone and the Active Metabolite Canrenone Concentrations and Adherence
Authors: Peeters Laura E. J. , Tjong Leonardien K. , Rietdijk Wim J. R. , van Gelder Teun , Koch Birgit C. P. , Versmissen Jorie
Published: January 2022

We aim to investigate sex differences in blood concentrations of spironolactone and the active metabolite canrenone in resistant hypertension patients. Furthermore, sex differences in adherence for spironolactone and other antihypertensive drugs (AHDs) were studied. The patients in this post hoc study had all participated in a single-blind randomized controlled trial called RHYME-RCT (Dutch Trial Register, NL6736). Concentrations in blood of several AHDs were assessed in RHYME-RCT to investigate adherence to treatment. This allowed for a comparison of drug exposure to spironolactone and canrenone between males and females. In linear regression models, no statistically significant sex differences (N = 35) in spironolactone (B =−10.23, SE = 7.92, p = 0.206) or canrenone (B = 1.24, SE = 10.96, p = 0.911) concentrations after adjustment for dose and time between sampling and intake were found. Furthermore, no statistically significant differences in non-adherence to spironolactone were found between sexes (N = 54, male 15% vs. female 38%, p = 0.100), but non-adherence to spironolactone was associated with non-adherence to other AHDs (p ≤ 0.001). Spironolactone and canrenone concentrations were not different between males and females with resistant hypertension. Although not statistically significant, females were twice as likely to be non-adherent to spironolactone compared to males, and thereby also more likely to be non-adherent to other AHDs.
Retrograde Analysis of Calcium Signaling by CaMPARI2 Shows Cytosolic Calcium in Chondrocytes Is Unaffected by Parabolic Flights
Authors: Hammer Andreas , Cerretti Geraldine , Ricciardi Dario A. , Schiffmann David , Maranda Simon , Kummer Raphael , Zumbühl Christoph , Rattenbacher-Kiser Karin F. , von Arx Silvan , Ammann Sebastian , Strobl Frederic , Berkane Rayene , Stolz Alexandra , Stelzer Ernst H. K. , Egli Marcel , Schleiff Enrico , Wuest Simon L. , Böhmer Maik
Published: January 2022

Calcium (Ca2+) elevation is an essential secondary messenger in many cellular processes, including disease progression and adaptation to external stimuli, e.g., gravitational load. Therefore, mapping and quantifying Ca2+ signaling with a high spatiotemporal resolution is a key challenge. However, particularly on microgravity platforms, experiment time is limited, allowing only a small number of replicates. Furthermore, experiment hardware is exposed to changes in gravity levels, causing experimental artifacts unless appropriately controlled. We introduce a new experimental setup based on the fluorescent Ca2+ reporter CaMPARI2, onboard LED arrays, and subsequent microscopic analysis on the ground. This setup allows for higher throughput and accuracy due to its retrograde nature. The excellent performance of CaMPARI2 was demonstrated with human chondrocytes during the 75th ESA parabolic flight campaign. CaMPARI2 revealed a strong Ca2+ response triggered by histamine but was not affected by the alternating gravitational load of a parabolic flight.
Zinc Homeostasis in Diabetes Mellitus and Vascular Complications
Authors: MacKenzie Stephanie , Bergdahl Andreas
Published: January 2022

Oxidative stress represents an impaired metabolic system that promotes damage to cells and tissues. This is the predominant factor that leads to the development and progression of diabetes and diabetic complications. Research has indicated that zinc plays a consequential mechanistic role in the protection against oxidative stress as zinc is required for the proper functioning of the antioxidant system, the suppression of inflammatory mediators, and the modulation of zinc transporters. Recently, the mechanisms surrounding ZnT8, ZIP7, and metallothionein have shown to be of particular pathogenic importance and are considered as potential therapeutic targets in disease management. The literature has shown that zinc dysregulation is associated with diabetes and may be considered as a leading contributor to the deleterious vascular alterations exhibited by the disease. Although further investigation is required, studies have indicated the favorable use of zinc supplementation in the protection against and prevention of oxidative stress and its consequences over the course of the condition. This review aims to provide a comprehensive account of zinc homeostasis, the oxidative mechanisms governed by zinc status, current therapeutic targets, and the impact of zinc supplementation in the prevention of disease onset and in mitigating vascular complications.
Digital E. coli Counter: A Microfluidics and Computer Vision-Based DNAzyme Method for the Isolation and Specific Detection of E. coli from Water Samples
Authors: Rauf Sakandar , Tashkandi Nouran , de Oliveira Filho José Ilton , Oviedo-Osornio Claudia Iluhí , Danish Muhammad S. , Hong Pei-Ying , Salama Khaled N.
Published: January 2022

Biological water contamination detection-based assays are essential to test water quality; however, these assays are prone to false-positive results and inaccuracies, are time-consuming, and use complicated procedures to test large water samples. Herein, we show a simple detection and counting method for E. coli in the water samples involving a combination of DNAzyme sensor, microfluidics, and computer vision strategies. We first isolated E. coli into individual droplets containing a DNAzyme mixture using droplet microfluidics. Upon bacterial cell lysis by heating, the DNAzyme mixture reacted with a particular substrate present in the crude intracellular material (CIM) of E. coli. This event triggers the dissociation of the fluorophore-quencher pair present in the DNAzyme mixture leading to a fluorescence signal, indicating the presence of E. coli in the droplets. We developed an algorithm using computer vision to analyze the fluorescent droplets containing E. coli in the presence of non-fluorescent droplets. The algorithm can detect and count fluorescent droplets representing the number of E. coli present in the sample. Finally, we show that the developed method is highly specific to detect and count E. coli in the presence of other bacteria present in the water sample.
Effects of Delivering Guanidinoacetic Acid or Its Prodrug to the Neural Tissue: Possible Relevance for Creatine Transporter Deficiency
Authors: Adriano Enrico , Salis Annalisa , Damonte Gianluca , Millo Enrico , Balestrino Maurizio
Published: January 2022

The creatine precursor guanidinoacetate (GAA) was used as a dietary supplement in humans with no adverse events. Nevertheless, it has been suggested that GAA is epileptogenic or toxic to the nervous system. However, increased GAA content in rodents affected by guanidinoacetate methyltransferase (GAMT) deficiency might be responsible for their spared muscle function. Given these conflicting data, and lacking experimental evidence, we investigated whether GAA affected synaptic transmission in brain hippocampal slices. Incubation with 11.5 μM GAA (the highest concentration in the cerebrospinal fluid of GAMT-deficient patients) did not change the postsynaptic compound action potential. Even 1 or 2 mM had no effect, while 4 mM caused a reversible decrease in the potential. Guanidinoacetate increased creatine and phosphocreatine, but not after blocking the creatine transporter (also used by GAA). In an attempt to allow the brain delivery of GAA when there was a creatine transporter deficiency, we synthesized diacetyl guanidinoacetic acid ethyl ester (diacetyl-GAAE), a lipophilic derivative. In brain slices, 0.1 mM did not cause electrophysiological changes and improved tissue viability after blockage of the creatine transporter. However, diacetyl-GAAE did not increase creatine nor phosphocreatine in brain slices after blockage of the creatine transporter. We conclude that: (1) upon acute administration, GAA is neither epileptogenic nor neurotoxic; (2) Diacetyl-GAAE improves tissue viability after blockage of the creatine transporter but not through an increase in creatine or phosphocreatine. Diacetyl-GAAE might give rise to a GAA–phosphoGAA system that vicariates the missing creatine–phosphocreatine system. Our in vitro data show that GAA supplementation may be safe in the short term, and that a lipophilic GAA prodrug may be useful in creatine transporter deficiency.
MaDoPO: Magnetic Detection of Positions and Orientations of Segmented Deep Brain Stimulation Electrodes: A Radiation-Free Method Based on Magnetoencephalography
Authors: Yalaz Mevlüt , Maling Nicholas , Deuschl Günther , Juárez-Paz León M. , Butz Markus , Schnitzler Alfons , Helmers Ann-Kristin , Höft Michael
Published: January 2022

Background: Current approaches to detect the positions and orientations of directional deep brain stimulation (DBS) electrodes rely on radiative imaging data. In this study, we aim to present an improved version of a radiation-free method for magnetic detection of the position and the orientation (MaDoPO) of directional electrodes based on a series of magnetoencephalography (MEG) measurements and a possible future solution for optimized results using emerging on-scalp MEG systems. Methods: A directional DBS system was positioned into a realistic head–torso phantom and placed in the MEG scanner. A total of 24 measurements of 180 s each were performed with different predefined electrode configurations. Finite element modeling and model fitting were used to determine the position and orientation of the electrode in the phantom. Related measurements were fitted simultaneously, constraining solutions to the a priori known geometry of the electrode. Results were compared with the results of the high-quality CT imaging of the phantom. Results: The accuracy in electrode localization and orientation detection depended on the number of combined measurements. The localization error was minimized to 2.02 mm by considering six measurements with different non-directional bipolar electrode configurations. Another six measurements with directional bipolar stimulations minimized the orientation error to 4°. These values are mainly limited due to the spatial resolution of the MEG. Moreover, accuracies were investigated as a function of measurement time, number of sensors, and measurement direction of the sensors in order to define an optimized MEG device for this application. Conclusion: Although MEG introduces inaccuracies in the detection of the position and orientation of the electrode, these can be accepted when evaluating the benefits of a radiation-free method. Inaccuracies can be further reduced by the use of on-scalp MEG sensor arrays, which may find their way into clinics in the foreseeable future.
Understanding Occupants’ Thermal Sensitivity According to Solar Radiation in an Office Building with Glass Curtain Wall Structure
Authors: Kim Sung-Kyung , Ryu Ji-Hye , Seo Hyun-Cheol , Hong Won-Hwa
Published: January 2022

The thermal comfort of occupants in the increasing number of modern buildings with glass curtain wall structures is of significant research interest. As the thermal sensitivity of building occupants varies with building features, situational factors, and the human body’s thermal balance, it is necessary to derive the comfort temperature based on field research, which was conducted in this study in a South Korean office building with a glass curtain wall structure. The influence of solar radiation on the indoor thermal environment and thermal comfort obtained by measurements and occupant questionnaires was analyzed using cumulative graphs and a sensitivity analysis. The observed changes in operative temperature over time confirmed that occupant comfort was significantly affected by the radiant temperature. Based on this result, two groups (Group A near the windows and Group B near the interior corridor) were defined for analysis. Owing to the influx of solar radiation, Group A was more sensitive to changes in the thermal environment (0.67/°C) than Group B (0.49/°C), and the derived comfort temperature for each group differed from the set temperature by approximately ±2 °C. Thus, it was confirmed that the solar radiation introduced through a glass curtain wall building has a direct impact on the indoor thermal environment and occupant comfort according to location.