bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Ethical Ideologies on Students’ Attitude toward Animals—A Pakistani Perspective
Authors: Khalid Asiya , Martens Pim , Khalid Aliya
Published: March 2023

Idealism and relativism are components of ethical ideologies which have been explored in relation to animal welfare and attitudes, and potential cultural differences. The present study investigated how ethical ideologies influenced attitude toward animals among undergraduate students. With the help of stratified random sampling, 450 participants were selected from both private and public sector universities in Pakistan. Research instruments consisted of a demographic sheet, the Ethics Position Questionnaire (EPQ), the Animal Attitude Scale—10-Item Version (AAS-10), and Animal Issue Scale (AIS). The study hypotheses were explored by employing various statistical analyses like Pearson Product Moment Correlation, independent sample t-test, ANOVA, and linear regression. Results revealed that there was a significant positive relationship between ethical ideologies (idealism and relativism) and attitude toward animals in students. Results further showed that students who consumed meat less frequently scored higher on relativism as compared to those who consumed meat more frequently (however, the effect size was small). It was also found that senior students held more idealistic ideologies as compared to freshman students. Finally, idealism positively predicted concern for animal welfare among students. The current study shed light on how ethical ideologies can shape and influence animal welfare. It further highlighted the potential cultural differences for the study variables by allowing for comparison with other published studies. By understanding these dynamics better, researchers will be better equipped to help students become informed citizens that may also influence future decision-making processes.
Clearance of Biodegradable Polymer and Polyethylene Films from the Rumens of Holstein Bull Calves
Authors: Galyon Hailey , Vibostok Samuel , Duncan Jane , Ferreira Gonzalo , Whittington Abby , Havens Kirk , McDevitt Jason , Cockrum Rebecca
Published: March 2023

Due to the occurrence of plastic impaction in ruminants and its deleterious effects on health and production, it is necessary to determine the suitability of biodegradable polymers to replace polyethylene-based agricultural plastics, such as hay netting. The objectives of this study were to evaluate the clearance of a polyhydroxyalkanoate (PHA) and poly(butylene succinate-co-adipate) (PBSA) melt-blend polymer from the rumen when fed to cattle and subsequent animal health. Twelve Holstein bull calves were dosed with an encapsulated 13.6 g of PBSA:PHA (Blend), 13.6 g of low-density polyethylene (LDPE), or four empty gelatin capsules (Control) for 30 d. The feed intake, body weight, and body temperature were evaluated, and hemograms were run on d 0 and d 30. On d 31, calves were euthanized to evaluate gross rumen measurements and pathology, papillae length, and polymer residues in rumen contents. No calves presented any signs related to plastic impaction. The feed intake; body weight; rectal temperature; hematological parameters; gross rumen measurements and pathology; and rumen pH and temperature were not affected by treatments. Calves dosed with LDPE had 27 g of undegraded polymer retained in the rumen while Blend calves had only 2 g of fragmented polymers that were 10% of their original size. Agricultural plastics developed from PBSA:PHA may be a suitable alternative to LDPE-based products in the case of animal ingestion and may reduce the incidence of plastic impaction.
A One-Year Retrospective Analysis of Viral and Parasitological Agents in Wildlife Animals Admitted to a First Aid Hospital
Authors: Pacini Maria Irene , Mazzei Maurizio , Sgorbini Micaela , D’Alfonso Rossella , Papini Roberto Amerigo
Published: March 2023

This study aimed to provide information on the presence and frequency of viral and parasitic agents in wildlife presented to a Veterinary Teaching Hospital in 2020–2021. Serum and faecal samples were collected from 50 rescued animals (roe deer, fallow deer, foxes, badgers, pine martens, and porcupines) and examined by serological, molecular, and parasitological techniques. Transtracheal wash (TTW) was also collected post-mortem from roe deer. Overall, the results of the different techniques showed infections with the following viral and parasitic agents: Bovine Viral Diarrhea Virus, Small Ruminant Lentiviruses, Kobuvirus, Astrovirus, Canine Adenovirus 1, Bopivirus, gastrointestinal strongyles, Capillaria, Ancylostomatidae, Toxocara canis, Trichuris vulpis, Hymenolepis, Strongyloides, Eimeria, Isospora, Dictyocaulus, Angiostrongylus vasorum, Crenosoma, Dirofilaria immitis, Neospora caninum, Giardia duodenalis, and Cryptosporidium. Sequencing (Tpi locus) identified G. duodenalis sub-assemblages AI and BIV in one roe deer and one porcupine, respectively. Adult lungworms collected from the TTW were identified as Dictyocaulus capreolus (COX1 gene). This is the first molecular identification of G. duodenalis sub-assemblage AI and D. capreolus in roe deer in Italy. These results show a wide presence of pathogens in wild populations and provide an overview of environmental health surveillance.
Effects of Bacillus coagulans and Lactobacillus plantarum on the Fermentation Characteristics, Microbial Community, and Functional Shifts during Alfalfa Silage Fermentation
Authors: Wang Yan , Ke Wencan , Lu Qiang , Zhang Guijie
Published: March 2023

This study aimed to investigate the potential of Bacillus coagulans (BC) as an inoculant in alfalfa silage fermentation. Fresh alfalfa was harvested at a dry matter (DM) content of 329.60 g/kg fresh weight (FW), and inoculated without (CON) or with BC (1 × 106 CFU/g FW), Lactobacillus plantarum (LP, 1 × 106 CFU/g FW), and their combinations (LP+BC, 1 × 106 CFU/g FW, respectively). Samples were taken at 3, 7, 14, 30, and 60 d, with three replicates for each. The prolonged ensiling period resulted in a decrease in pH values and an increase in lactic acid (LA) concentrations in alfalfa silages. After 60 d of fermentation, the application of BC and LP decreased the pH values and increased LA concentrations in treated silages, especially when their combination was applied. Application of BC preserved more water-soluble carbohydrates (WSC), and further application of BC increased WSC in LP+BC-treated silage compared to LP-treated silage. There was no significant difference in the crude protein (CP) content between the CON and treated silages, however, the BC and LP treatments reduced the ammonia nitrogen (NH3-N) concentration, especially when their combination was applied. Additionally, the BC and LP-treated silages had lower neutral detergent fiber (NDF) and acid detergent fiber (ADF) when compared to the CON silage (p < 0.001). Inoculants also increased Lactobacillus abundance and decreased Enterococcus abundance after 60 d of fermentation. Spearman’s rank correlation analysis revealed a positive correlation between LA concentration and Lactobacillus abundance. It was noteworthy that LP, BC, and their combination increased the relative abundances of carbohydrate metabolism, energy metabolism, cofactors, and vitamin metabolism, decreasing the relative abundances of amino acid metabolism and drug resistance: antimicrobial. Therefore, the inclusion of BC increased the fermentation quality of alfalfa silage, with the optimal combination being LP+BC. According to the findings, BC could be considered a viable bioresource for improving fermentation quality.
Microbial Fermented Liquid Supplementation Improves Nutrient Digestibility, Feed Intake, and Milk Production in Lactating Dairy Cows Fed Total Mixed Ration
Authors: Polyorach Sineenart , Nampukdee Rutsamee , Wanapat Metha , Kang Sungchhang , Cherdthong Anusorn , Poungchompu Onanong , Gunun Pongsatorn , Gunun Nirawan , Foiklang Suban , Thinowong Anusorn , Jindatajak Yaungyote , Lapmee Anuwat , Norrapoke Thitima
Published: March 2023

The purpose of this experiment was to examine the effects of MFL supplementation on feed intake, nutrient digestibility, milk yield, and milk composition in early lactating dairy cows. Twelve, Thai crossbred Holstein Friesian cows in early lactation, 500 ± 30 kg of body weight (BW), were randomly assigned according to a completely randomized design (CRD). MFL supplementation levels of 0, 100, 200, and 300 mL/d were used as treatments. Experimental animals were fed a total mixed ration (TMR) with a roughage to concentrate ratio (R:C ratio) of 40:60, which contains 12% crude protein (CP) and 70% total digestible nutrient (TDN). Rice straw was a roughage source. MFL supplementation levels had no effect (p > 0.05) on body weight change and dry matter intake (DMI) expressed as %BW, whereas DMI expressed as metabolic body weight (BW0.75) was linearly (p < 0.05) increased, with the highest at 200 mL/d in the YFL supplementation group (147.5 g/kg BW0.75), whereas feed intake of organic matter (OM), CP, ether extract (EE), neutral detergent fiber (NDF) and acid detergent fiber (ADF) did not significant (p > 0.05) difference among treatments. Related to apparent digestibility, MFL levels did not affect (p > 0.05) on DM, OM, and EE digestibility, while apparent digestibility of CP, NDF, and ADF were linearly increased (p < 0.05) when increasing MFL supplementation levels, and the highest (p < 0.05) were the 200 and 300 mL/d FML supplemented groups. BUN at 0 h post feeding did not show a significant difference (p > 0.05) between treatments, while at 4 h after feeding, BUN was linearly (p < 0.05) increased from 0, 100, 200, and 300 (mL/day) MFL supplementation, the values were 12.9, 13.1, 19.7, and 18.4 mg/dL, respectively and the highest was 200 mL/head/day for the MFL supplemented group. MFL supplementation did not affect (p > 0.05) milk fat, lactose, solid not fat (SNF), and specific gravity of milk, while MFL supplementation at 200 mL/day caused a linear increase (p < 0.01) in BUN, MUN, milk yield, milk protein, total solids (TS) and 3.5% FCM when supplement levels were increased. In conclusion, MFL supplementation in early lactating dairy cows could improve feed intake, nutrient digestibility, milk yield, and milk composition.
Estimates of Genetic Parameters for Direct and Maternal Effects on Pre-Weaning Growth Traits in Turkish Saanen Kids
Authors: Erdoğan Ataç Funda , Takma Çiğdem , Gevrekci Yakut , Öziş Altınçekiç Şeniz , Ayaşan Tugay
Published: March 2023

Data on 4487 Turkish Saanen kids from 176 bucks and 1318 dam-goats, obtained from the Turkish Saanen goats in the Izmir region and collected between 2018 and 2019, were analyzed to examine the effect of genetic and non-genetic factors on growth traits. The average birth weight of the kids was determined as 3.33 ± 0.68 kg, the average W60 was 13.06 ± 2.94 kg, the average WW was 18.38 ± 4.14 kg, and the average PreWDG until weaning was 0.17 ± 0.04 g. Model 1, which does not account for the maternal effect, and Model 2, which includes the maternal effect, were used in the estimation of genetic parameters. The heritability estimates of BW, W60, WW, and PreWDG ranged from 0.05 to 0.59 in both models. It is recommended to consider the maternal effect as well as the environmental factors in the selection program for the best early breeder selection of kids growing alongside their mothers until the weaning period.
Molecular Cytogenetics in Domestic Bovids: A Review
Authors: Iannuzzi Alessandra , Iannuzzi Leopoldo , Parma Pietro
Published: March 2023

The discovery of the Robertsonian translocation (rob) involving cattle chromosomes 1 and 29 and the demonstration of its deleterious effects on fertility focused the interest of many scientific groups on using chromosome banding techniques to reveal chromosome abnormalities and verify their effects on fertility in domestic animals. At the same time, comparative banding studies among various species of domestic or wild animals were found useful for delineating chromosome evolution among species. The advent of molecular cytogenetics, particularly the use of fluorescence in situ hybridization (FISH), has allowed a deeper investigation of the chromosomes of domestic animals through: (a) the physical mapping of specific DNA sequences on chromosome regions; (b) the use of specific chromosome markers for the identification of the chromosomes or chromosome regions involved in chromosome abnormalities, especially when poor banding patterns are produced; (c) better anchoring of radiation hybrid and genetic maps to specific chromosome regions; (d) better comparisons of related and unrelated species by comparative FISH mapping and/or Zoo-FISH techniques; (e) the study of meiotic segregation, especially by sperm-FISH, in some chromosome abnormalities; (f) better demonstration of conserved or lost DNA sequences in chromosome abnormalities; (g) the use of informatic and genomic reconstructions, in addition to CGH arrays, to predict conserved or lost chromosome regions in related species; and (h) the study of some chromosome abnormalities and genomic stability using PCR applications. This review summarizes the most important applications of molecular cytogenetics in domestic bovids, with an emphasis on FISH mapping applications.
Green Synthesis of Na abietate Obtained from the Salification of Pinus elliottii Resin with Promising Antimicrobial Action
Authors: Schons Aline B. , Appelt Patrícia , Correa Jamille S. , Cunha Mário A. A. , Rodrigues Mauricio G. , Anaissi Fauze J.
Published: March 2023

The growing concern about the emergence of increasingly antibiotic-r4esistant bacteria imposes the need to search and develop drugs to combat these microorganisms. This, combined with the search for low-cost synthesis methods, was the motivation for the elaboration of this work. Abietic acid present in the resin of Pinus elliotti var. elliotti was used to generate a sodium salt by salification. The synthesis route was low-cost, consisting of only two reaction steps at mild temperatures without toxic organic solvents, and eco-friendly and easy to conduct on an industrial scale. Sodium abietate (Na-C20H29O2) was characterized by mass spectrometry, infrared spectroscopy, elemental analysis, X-ray diffraction, scanning electron microscopy, and energy-dispersive spectroscopy. To perform the antimicrobial tests, the determination of minimum inhibitory concentration and the disk diffusion assay was performed. The results obtained showed that the salt Na abietate performed an antimicrobial action against the bacterial strains S. aureus, E. coli, L.monocytogenes, and S. enterica Typhimurium and the yeast C. albicans. The disk diffusion test showed a high inhibition potential against S. enterica compared to the standard antimicrobial tetracycline, as an inhibition index of 1.17 was found. For the other bacterial strains, the inhibition values were above 40%. The MIC test showed promising results in the inhibition of E. coli, L. monocytogenes, and C. albicans, indicating bacteriostatic activity against the first microorganism and bactericidal and fungicidal activities against the others. Therefore, the results showed the action of Na abietate as a possible effective antimicrobial drug, highlighting its sustainability within a circular economy.
Epigenetic and Drug Response Modulation of Epigalocaten-In-3-Gallate in Staphylococcus aureus with Divergent Resistance Phenotypes
Authors: Mira Ana Rita , Zeferino Ana Sofia , Inácio Raquel , Delgadinho Mariana , Brito Miguel , Calado Cecília R. C. , Ribeiro Edna
Published: March 2023

Healthcare-associated methicillin-resistant Staphylococcus aureus infections represent extremely high morbidity and mortality rates worldwide. We aimed to assess the antimicrobial potential and synergistic effect between Epigalocatenin-3-gallate (EGCG) and different antibiotics in S. aureus strains with divergent resistance phenotypes. EGCG exposure effects in epigenetic and drug resistance key modulators were also evaluated. S. aureus strains (n = 32) were isolated from infected patients in a Lisbon hospital. The identification of the S. aureus resistance phenotype was performed through automatized methods. The antibiotic synergistic assay was performed through disk diffusion according to EUCAST guidelines with co-exposure to EGCG (250, 100, 50 and 25 µg/mL). The bacteria’s molecular profile was assessed through FTIR spectroscopy. The transcriptional expression of OrfX, SpdC and WalKR was performed by using qRT-PCR. FTIR-spectroscopy analysis enabled the clear discrimination of MRSA/MSSA strains and the EGCG exposure effect in the bacteria’s molecular profiles. Divergent resistant phenotypes were associated with divergent transcriptional expression of the epigenetic modulator OrfX, particularly in MRSA strains, as well as the key drug response modulators SpdC and WalKR. These results clearly demonstrate that EGCG exposure alters the expression patterns of key epigenetic and drug response genes with associated divergent-resistant profiles, which supports its potential for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms.
The Genotypic and Phenotypic Characteristics Contributing to Flomoxef Sensitivity in Clinical Isolates of ESBL-Producing E. coli Strains from Urinary Tract Infections
Authors: Sakaeda Kazuma , Sadahira Takuya , Maruyama Yuki , Iwata Takehiro , Watanabe Masami , Wada Koichiro , Araki Motoo
Published: March 2023

We carried out a molecular biological analysis of extended-spectrum β-lactamase (ESBL)-producing E. coli strains and their sensitivity to flomoxef (FMOX). Sequence type (ST) analysis by multilocus sequence typing (MLST) and classification of ESBL genotypes by multiplex PCR were performed on ESBL-producing E. coli strains isolated from urine samples collected from patients treated at our institution between 2008 and 2018. These sequences were compared with results for antimicrobial drug susceptibility determined using a micro-liquid dilution method. We also analyzed cases treated with FMOX at our institution to examine its clinical efficacy. Of the 911 E. coli strains identified, 158 (17.3%) were ESBL-producing. Of these, 67.7% (107/158) were strain ST-131 in ST analysis. Nearly all (154/158; 97.5%) were CTX-M genotypes, with M-14 and M-27 predominating. The isolated strains were sensitive to FMOX in drug susceptibility tests. Among the patient samples, 33 cases received FMOX, and of these, 5 had ESBL-producing E. coli. Among these five cases, three received FMOX for surgical prophylaxis as urinary carriers of ESBL-producing E. coli, and postoperative infections were prevented in all three patients. The other two patients received FMOX treatment for urinary tract infections. FMOX treatment was successful for one, and the other was switched to carbapenem. Our results suggest that FMOX has efficacy for perioperative prophylactic administration in urologic surgery involving carriers of ESBL-producing bacteria and for therapeutic administration for urinary tract infections. Use of FMOX avoids over-reliance on carbapenems or β-lactamase inhibitors and thus is an effective antimicrobial countermeasure.
Solid Lipid Nanoparticles: Review of the Current Research on Encapsulation and Delivery Systems for Active and Antioxidant Compounds
Authors: Subroto Edy , Andoyo Robi , Indiarto Rossi
Published: March 2023

Various active compounds are easily damaged, so they need protection and must be easily absorbed and targeted. This problem can be overcome by encapsulating in the form of solid lipid nanoparticles (SLNs). Initially, SLNs were widely used to encapsulate hydrophobic (non-polar) active compounds because of their matched affinity and interactions. Currently, SLNs are being widely used for the encapsulation of hydrophilic (polar) and semipolar active compounds, but there are challenges, including increasing their entrapment efficiency. This review provides information on current research on SLNs for encapsulation and delivery systems for active and antioxidant compounds, which includes various synthesis methods and applications of SLNs in various fields of utilization. SLNs can be developed starting from the selection of solid lipid matrices, emulsifiers/surfactants, types of active compounds or antioxidants, synthesis methods, and their applications or utilization. The type of lipid used determines crystal formation, control of active compound release, and encapsulation efficiency. Various methods can be used in the SLN fabrication of active compounds and hydrophilic/hydrophobic antioxidants, which have advantages and disadvantages. Fabrication design, which includes the selection of lipid matrices, surfactants, and fabrication methods, determines the characteristics of SLNs. High-shear homogenization combined with ultrasonication is the recommended method and has been widely used because of the ease of preparation and good results. Appropriate fabrication design can produce SLNs with stable active compounds and antioxidants that become suitable encapsulation systems for various applications or uses.
Mechanism of Action of Natural Compounds in Peripheral Multiorgan Dysfunction and Hippocampal Neuroinflammation Induced by Sepsis
Authors: D’Amico Ramona , Tomasello Mario , Impellizzeri Daniela , Cordaro Marika , Siracusa Rosalba , Interdonato Livia , Abdelhameed Ali Saber , Fusco Roberta , Calabrese Vittorio , Cuzzocrea Salvatore , Di Paola Rosanna
Published: March 2023

Bacterial sepsis induces the production of excessive pro-inflammatory cytokines and oxidative stress, resulting in tissue injury and hyperinflammation. Patients recovering from sepsis have increased rates of central nervous system (CNS) morbidities, which are linked to long-term cognitive impairment, such as neurodegenerative pathologies. This paper focuses on the tissue injury and hyperinflammation observed in the acute phase of sepsis and on the development of long-term neuroinflammation associated with septicemia. Here we evaluate the effects of Coriolus versicolor administration as a novel approach to treat polymicrobial sepsis. Rats underwent cecal ligation and perforation (CLP), and Coriolus versicolor (200 mg/kg in saline) was administered daily by gavage. Survival was monitored, and tissues from vital organs that easily succumb to infection were harvested after 72 h to evaluate the histological changes. Twenty-eight days after CLP, behavioral analyses were performed, and serum and brain (hippocampus) samples were harvested at four weeks from surgery. Coriolus versicolor increased survival and reduced acute tissue injury. Indeed, it reduced the release of pro-inflammatory cytokines in the bloodstream, leading to a reduced chronic inflammation. In the hippocampus, Coriolus versicolor administration restored tight junction expressions, reduce cytokines accumulation and glia activation. It also reduced toll-like receptor 4 (TLR4) and neuronal nitric oxide synthase (nNOS) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome components expression. Coriolus versicolor showed antioxidant activities, restoring glutathione (GSH) levels and catalase and superoxide dismutase (SOD) activities and reducing lipid peroxidation, nitrite and reactive oxygen species (ROS) levels. Importantly, Coriolus versicolor reduced amyloid precursor protein (APP), phosphorylated-Tau (p-Tau), pathologically phosphorylated tau (PHF1), phosphorylated tau (Ser202 and Thr205) (AT8), interferon-induced transmembrane protein 3 (IFITM3) expression, and β-amyloid accumulation induced by CLP. Indeed, Coriolus versicolor restored synaptic dysfunction and behavioral alterations. This research shows the effects of Coriolus versicolor administration on the long-term development of neuroinflammation and brain dysfunction induced by sepsis. Overall, our results demonstrated that Coriolus versicolor administration was able to counteract the degenerative process triggered by sepsis.
Effects of Different Hydration Strategies in Young Men during Prolonged Exercise at Elevated Ambient Temperatures on Pro-Oxidative and Antioxidant Status Markers, Muscle Damage, and Inflammatory Status
Authors: Pałka Tomasz , Koteja Piotr Michał , Tota Łukasz , Rydzik Łukasz , Leiva-Arcas Alejandro , Kałuża Anna , Czarny Wojciech , Ambroży Tadeusz
Published: March 2023

Physical exercise is associated with an increase in the speed of metabolic processes to supply energy to working muscles and endogenous heat production. Intense sweating caused by the work performed at high ambient temperatures is associated with a significant loss of water and electrolytes, leading to dehydration. This study aimed to examine the effectiveness of different hydration strategies in young men during prolonged exercise at elevated ambient temperatures on levels of pro-oxidative and antioxidant status, oxidative status markers (TAC/TOC), muscle cell damage (Mb, LDH), and inflammatory status (WBC, CRP, IL-1β). The study was conducted on a group of 12 healthy men with average levels of aerobic capacity. The intervention consisted of using various hydration strategies: no hydration; water; and isotonic drinks. The examination was di-vided into two main stages. The first stage was a preliminary study that included medical exami-nations, measurements of somatic indices, and exercise tests. The exercise test was performed on a cycle ergometers. Their results were used to determine individual relative loads for the main part of the experiment. In the second stage, the main study was conducted, involving three series of weekly experimental tests using a cross-over design. The change in plasma volume (∆PV) measured im-mediately and one hour after the exercise test was significantly dependent on the hydration strategy (p = 0.003 and p = 0.002, respectively). The mean values of oxidative status did not differ signifi-cantly between the hydration strategy used and the sequence in which the test was performed. Using isotonic drinks, due to the more efficient restoration of the body’s water and electrolyte balance compared to water or no hydration, most effectively protects muscle cells from the negative effects of exercise, leading to heat stress of exogenous and endogenous origin.
Neuroprotective Effects of Nano-Curcumin against Cypermethrin Associated Oxidative Stress and Up-Regulation of Apoptotic and Inflammatory Gene Expression in Rat Brains
Authors: Ashafaq Mohammad , Hussain Sohail , Alshahrani Saeed , Siddiqui Rahimullah , Alam Mohammad Intakhab , Elhassan Taha Manal Mohamed , Almoshari Yosif , Alqahtani Saad S. , Jali Abdulmajeed M. , Aljohani Hashim M.
Published: March 2023

Cypermethrin (CPM) is the most toxic synthetic pyrethroid that has established neurotoxicity through oxidative stress and neurochemical agitation in experimental rats. The toxic effects are supposed to be mediated by modifying the sodium channels, reducing Na-K ATPase, acetylcholine esterase (AchE), and monoamine oxidase (MAO). The use of curcumin nanoparticles (NC) that have potent antioxidant, anti-inflammatory and antiapoptotic properties with improved bioavailability attenuates neurotoxicity in rat brains. To test this hypothesis, animals were divided into five groups, each having six animals. Group-I control received vehicle only, while Group-II was treated with 50 mg/kg CPM. Group-III and Group-IV received both CPM and NC 2.5 mg/kg and 5 mg/kg, respectively. Group-V received 5 mg of NC alone. The CPM and NC were given by oral route. Afterwards, brain antioxidant status was measured by assessing lipid peroxidation (LPO), 4-HNE, glutathione reduced (GSH), antioxidant enzyme catalase, and superoxide dismutase (SOD) along with neurotoxicity markers Na-K ATPase, AchE, and MAO. Inflammation and apoptosis indices were estimated by ELISA, qRT-PCR, and immunohistochemistry, while morphologic changes were examined by histopathology. Observations from the study confirmed CPM-induced neurotoxicity by altering Na-K ATPase, AchE, and MAO, and by decreasing the activity of antioxidant enzymes and GSH. Oxidative stress marker LPO and the level of inflammatory interleukins IL-6, IL-1β, and TNF-α were notably high, and elevated expressions of Bax, NF-kB, and caspase-3 and -9 were reported in CPM group. However, NC treatment against CPM offers protection by improving antioxidant status and lowering LPO, inflammation, and apoptosis. The neurotoxicity marker’s enzyme successfully attenuated after NC treatment. Therefore, this study supports the administration of NC effectively ameliorated CPM-induced neurotoxicity in experimental rats.
H2S Donors with Cytoprotective Effects in Models of MI/R Injury and Chemotherapy-Induced Cardiotoxicity
Authors: Hu Qiwei , Lukesh John C.
Published: March 2023

Hydrogen sulfide (H2S) is an endogenous signaling molecule that greatly influences several important (patho)physiological processes related to cardiovascular health and disease, including vasodilation, angiogenesis, inflammation, and cellular redox homeostasis. Consequently, H2S supplementation is an emerging area of interest, especially for the treatment of cardiovascular-related diseases. To fully unlock the medicinal properties of hydrogen sulfide, however, the development and refinement of H2S releasing compounds (or donors) are required to augment its bioavailability and to better mimic its natural enzymatic production. Categorizing donors by the biological stimulus that triggers their H2S release, this review highlights the fundamental chemistry and releasing mechanisms of a range of H2S donors that have exhibited promising protective effects in models of myocardial ischemia-reperfusion (MI/R) injury and cancer chemotherapy-induced cardiotoxicity, specifically. Thus, in addition to serving as important investigative tools that further advance our knowledge and understanding of H2S chemical biology, the compounds highlighted in this review have the potential to serve as vital therapeutic agents for the treatment (or prevention) of various cardiomyopathies.
Advances of H2S in Regulating Neurodegenerative Diseases by Preserving Mitochondria Function
Authors: Zhou Lina , Wang Qiang
Published: March 2023

Neurotoxicity is induced by different toxic substances, including environmental chemicals, drugs, and pathogenic toxins, resulting in oxidative damage and neurodegeneration in mammals. The nervous system is extremely vulnerable to oxidative stress because of its high oxygen demand. Mitochondria are the main source of ATP production in the brain neuron, and oxidative stress-caused mitochondrial dysfunction is implicated in neurodegenerative diseases. H2S was initially identified as a toxic gas; however, more recently, it has been recognized as a neuromodulator as well as a neuroprotectant. Specifically, it modulates mitochondrial activity, and H2S oxidation in mitochondria produces various reactive sulfur species, thus modifying proteins through sulfhydration. This review focused on highlighting the neuron modulation role of H2S in regulating neurodegenerative diseases through anti-oxidative, anti-inflammatory, anti-apoptotic and S-sulfhydration, and emphasized the importance of H2S as a therapeutic molecule for neurological diseases.
Antioxidant Status and Biotechnological Potential of New Vischeria vischeri (Eustigmatophyceae) Soil Strains in Enrichment Cultures
Authors: Yakoviichuk Aleksandr , Krivova Zinaida , Maltseva Svetlana , Kochubey Angelica , Kulikovskiy Maxim , Maltsev Yevhen
Published: March 2023

The functional state of enrichment cultures of the Eustigmatophycean strains Vischeria vischeri MZ–E3 and MZ–E4 after 25-day cultivation in the BBM medium was studied. The concentrations of chlorophyll a, total carotenoids, protein, vitamins A and E, fatty acid peroxidation product content, an antioxidant enzyme, and succinate dehydrogenase activity were measured. MZ–E3 succinate dehydrogenase activity was significantly higher by 2.21 times; the MZ–E4 strain had 2.94 times higher glutathione peroxidase activity. The MZ–E3 antioxidant activity index and the MZ–E3 unsaturation of fatty acids were 1.3 and 1.25 times higher than the MZ–E4. The retinol and α-tocopherol content of the MZ–E3 was 28.6% and 38.76% higher than MZ–E4. The main fatty acid profile differences were the 3.46-fold and 3.92-fold higher stearic and eicosapentaenoic acid content in the MZ–E4 biomass. MZ–E3 had higher antioxidant, energy, and metabolic and photosynthetic status than MZ–E4. The antioxidant status of the studied strains showed the dependence of the adaptive mechanisms of each, associated with differences in the ecological conditions of the biotopes from which they were isolated. These strains are promising for producing α-tocopherol and biomass enriched with omega-3 and omega-6 fatty acids.
Rethinking the Operation Pattern for Anomaly Detection in Industrial Cyber–Physical Systems
Authors: Cheng Zishuai , Cui Baojiang , Fu Junsong
Published: March 2023

Anomaly detection has been proven to be an efficient way to detect malicious behaviour and cyberattacks in industrial cyber–physical systems (ICPSs). However, most detection models are not entirely adapted to the real world as they require intensive computational resources and labelled data and lack interpretability. This study investigated the traffic behaviour of a real coal mine system and proposed improved features to describe its operation pattern. Based on these features, this work combined the basic deterministic finite automaton (DFA) and normal distribution (ND) models to build an unsupervised anomaly detection model, which uses a hierarchical structure to pursue interpretability. To demonstrate its capability, this model was evaluated on real traffic and seven simulated attack types and further compared with nine state-of-the-art works. The evaluation and comparison results show that the proposed method achieved a 99% F1-score and is efficient in detecting sophisticated attacks. Furthermore, it achieved an average 17% increase in precision and a 12% increase in F1-Score compared to previous works. These results confirm the advantages of the proposed method. The work further suggests that future works should investigate operation pattern features rather than pursuing complex algorithms.
Non-Extensive Statistical Mechanics in Acoustic Emissions: Detection of Upcoming Fracture in Rock Materials
Authors: Loukidis Andronikos , Stavrakas Ilias , Triantis Dimos
Published: March 2023

Acoustic emission (AE), recorded during uniaxial compressive loading with constantly increasing stress and stepped stress increments until the fracture of prismatic marble specimens, were analyzed in terms of non-extensive statistical mechanics (NESM). Initially introduced by Tsallis, NESM has proven to be an autonomous robust theoretical framework for studying fracture mechanisms and damage evolution processes during fracture experiments in specimens made of brittle materials. In the current work, the time intervals of the recorded AE data are analyzed in terms of NESM. For each examined specimen, the corresponding q entropic indices and the βq parameters were calculated, and their variability in terms of the stress applied were studied. Furthermore, a possible linear relationship between the entropic index q and the parameter βq was examined, and it was investigated whether the observed deviation from monotonicity between q and βq may signal increased accumulation of damage, eventually leading to the final fracture of the specimens. Through this work, the emergence of an additional pre-failure indicator (i.e., the deviation from monotonicity between q and βq) alongside well-established ones can provide further insight regarding the underlying crack development mechanisms and damage accumulation processes during the fracture of rock materials.
Evaluation on Lateral Stability of Vehicle: Impacts of Pavement Rutting, Road Alignment, and Adverse Weather
Authors: Tian Gang , Jia Yanshun , Chen Zeqi , Gao Ying , Wang Shaoquan , Wei Ziyao , Chen Yufei , Zhang Tianshuo
Published: March 2023

The combination of pavement rutting, poor road alignment, and extreme adverse weather will seriously threaten the driving safety of vehicles, whereas only a few of these factors are commonly concerned. This study aims to efficiently evaluate the impacts of various driving conditions on the lateral stability of the vehicle and produce a practical recommendation for pavement maintenance in what concerns rutting. A systematic framework was, thus, developed to conduct a comprehensive evaluation of the lateral stability of the vehicle, which incorporates a single-factor test and multi-factor test based on the stability indicators obtained from Carsim simulations. The vehicle road weather model was established in the Carsim software by considering seven factors, including driving speed, width–height ratio (WHR) of rutting sidewall, radius of circular curve, superelevation, crosswind angle, crosswind speed, and friction coefficient, respectively. The results show that the established framework behaves with satisfactory performance, regarding evaluating the effect of various impact factors on the lateral stability of the vehicle while driving across rutting. Stability indicators suddenly fluctuate in a short time, due to the instantaneous wandering behavior of crossing rutting. Additionally, the sudden fluctuation phenomenon is greatly enlarged, and the vehicle is inclined to occur with lateral instability when WHR equals 5, particularly in roll-over instability. It is recommended to concurrently confine the WHR greater than 10 and friction coefficient greater than 0.4, in order to ensuring driving stability. The multi-factor test revealed that the vehicle speed and WHR of the rutting are leading factors that affect driving stability, followed by the radius of circular curve, superelevation, crosswind angle, crosswind speed and friction coefficient, respectively, which are both essential factors for driving stability. The outcomes of this study may contribute to supplying guidelines for controlling key adverse conditions and making decisions on pavement maintenance.
A Novel Open-Closed-Loop Control Strategy for Quadrotor Trajectory Tracking on Real-Time Control and Acquisition Platform
Authors: Chen Keming , Ye Chaoyu , Wu Changke , Wang Hui , Jin Lei , Zhu Fenghao , Hong Hui
Published: March 2023

This paper presents a novel closed-loop control strategy that can be applied to quadrotor trajectory tracking to improve the control accuracy and stability. Inspired by intelligent creatures, the composite control strategy combines the open-loop mode and closed-loop control to overcome some disadvantages of oscillation and overshoot under feedback control mechanisms. The open-loop mode was realized by the quadrotor maneuverability model, which is proposed to build the bridge between the flight control commands and the desired acceleration. Then, a real-time control and acquisition platform with high-precision positioning, low-delay communication, and asynchronous distribution was built to collect the UAV’s real-time flight trajectory data for training the maneuverability model and to control the quadrotors asynchronously. Based on the platform and the trained model, the composite control mode on the closed-loop of the Proportional–Integral–Derivative (PID) control and the open-loop of the Long Short-Term Memory (LSTM) maneuverability model was implemented and verified. The experimental results show that the open-loop control strategy has a better advantage in the unity of time and space over the existing techniques for improving the UAV dynamic obstacle avoidance capability.
Analytical Prediction of Tunnel Deformation Beneath an Inclined Plane: Complex Potential Analysis
Authors: Mabe Fogang Pieride , Liu Yang , Zhao Jia-Le , Ka Thierno Aliou , Xu Shuo
Published: March 2023

When excavating a tunnel, the stresses are distributed asymmetrically along the tunnel cross-section. Other factors, particularly slope friction force and excavation speed, can also contribute to the deformation and displacement of a tunnel. Despite this, several authors have used the complex potential method to predict the ground deformation surrounding the tunnel. However, their applicability to the ground response caused by the asymmetric stress distribution around the tunnel wall is analysed in this context. This paper, therefore, proposes an approximate solution on the slope to predict the tunnel cross-section deformation. The solution is based on the complex potential method to predict analytically and numerically the ground deformation around the tunnel. However, two variables called the “complex potential functions” for the Laurent series expansion are used for the stress redistribution to the tunnel boundary conditions. Data from the Qijiazhuang tunnel case are used to justify the proposed analytical solutions. This solution is an essential guide for analyzing deformations in complex geological conditions and structures, such as steeper slopes.
Tunable Spatial Resolution Focused Laser Differential Interferometer for Density Fluctuation Measurement
Authors: Li Hongxun , Li Yudong , Chen Li , Yao Xianghong
Published: March 2023

We first propose and demonstrate a novel approach for achieving a focused laser differential interferometer (FLDI) system with tunable spatial resolution. The spatial resolution of the FLDI can be adjusted continuously between 83 μm and 382 μm. The density fluctuation of a supersonic shear flow is measured using the FLDI system with a spatial resolution of 182 μm, and the density fluctuations at different locations of the supersonic shear flow are measured and analyzed. The ability to adjust the spatial resolution in this work is of great significance for enhancing the spatial resolution and flexibility of the FLDI system.
A Convolutional Autoencoder Approach for Boosting the Specificity of Retinal Blood Vessels Segmentation
Authors: Nikoloulopoulou Natalia , Perikos Isidoros , Daramouskas Ioannis , Makris Christos , Treigys Povilas , Hatzilygeroudis Ioannis
Published: March 2023

Automated retina vessel segmentation of the human eye plays a vital role as it can significantly assist ophthalmologists in identifying many eye diseases, such as diabetes, stroke, arteriosclerosis, cardiovascular disease, and many other human illnesses. The fast, automatic and accurate retina vessel segmentation of the eyes is very desirable. This paper introduces a novel fully convolutional autoencoder for the retina vessel segmentation task. The proposed model consists of eight layers, each consisting of convolutional2D layers, MaxPooling layers, Batch Normalisation layers and more. Our model has been trained and evaluated on DRIVE and STARE datasets with 35 min of training time. The performance of the autoencoder model we introduce is assessed on two public datasets, the DRIVE and the STARE and achieved quite competitive results compared to the state-of-the-art methods in the literature. In particular, our model reached an accuracy of 95.73, an AUC_ROC of 97.49 on the DRIVE dataset, and an accuracy of 96.92 and an AUC ROC of 97.57 on the STARE dataset. Furthermore, our model has demonstrated the highest specificity among the methods in the literature, reporting a specificity of 98.57 on the DRIVE and 98.7 on the STARE dataset, respectively. The above statement can be noticed in the final blood vessel segmentation images produced by our convolutional autoencoder method since the segmentations are more accurate, sharp and noiseless than the result images of other proposed methods.
The Extended Information Systems Success Measurement Model: e-Learning Perspective
Authors: Vuckovic Teodora , Stefanovic Darko , Ciric Lalic Danijela , Dionisio Rogério , Oliveira Ângela , Przulj Djordje
Published: March 2023

This study investigated the crucial factors for measuring the success of the information system used in the e-learning process, considering the transformations in the work environment. This study was motivated by the changes caused by COVID-19 witnessed after the shift to fully online learning environments supported by e-learning systems, i.e., learning emphasized with information systems. Empirical research was conducted on a sample comprising teaching staff from two European universities: the University of Novi Sad, Faculty of Technical Sciences in Serbia and the Polytechnic Institute of Castelo Branco in Portugal. By synthesizing knowledge from review of the prior literature, supported by the findings of this study, the authors propose an Extended Information System Success Measurement Model—EISSMM. EISSMM underlines the importance of workforce agility, which includes the factors of proactivity, adaptability, and resistance to change, in the information system performance measurement model. The results of our research provide more extensive evidence and findings for scholars and practitioners that could support measuring information system success primarily in e-learning and other various contextual settings, highlighting the importance of people’s responses to work environment changes.
CA-CGNet: Component-Aware Capsule Graph Neural Network for Non-Rigid Shape Correspondence
Authors: Lian Yuanfeng , Chen Mengqi
Published: March 2023

3D non-rigid shape correspondence is significant but challenging in computer graphics, computer vision, and related fields. Although some deep neural networks have achieved encouraging results in shape correspondence, due to the complexity of the local deformation of non-rigid shapes, the ability of these networks to identify the spatial changes of objects is still insufficient. In this paper, we design a Component-aware Capsule Graph Network (CA-CGNet) to further address the features of embedding space based on the component constraints. Specifically, the dynamic clustering strategy is used to classify the features of patches produced by over-segmentation in order to further reduce noise interference. Moreover, aiming at the problem that existing routing ignores the embedding relationship between capsules, we propose a component-aware capsule graph routing to fully describe the relationship between capsules, which regards capsules as nodes in the graph network and constrains nodes through component information. Then, a knowledge distillation strategy is introduced to improve the convergence speed of the network by decreasing the parameters while maintaining accuracy. Finally, a component pair constraint is added to the functional map, and the component-based semantic loss function is proposed, which can compute isomeric in both direct and symmetric directions. The experimental results show that CA-CGNet improves by 10.21% compared with other methods, indicating the accuracy, generalization, and efficiency of our method on the FAUST, SCAPE, TOSCA, and KIDS datasets.
The Impact of Gamifications and Serious Games on Driving under Unfamiliar Traffic Regulations
Authors: Alyamani Hasan , Alharbi Nesreen , Roboey Amjad , Kavakli Manolya
Published: March 2023

Drivers face many challenges when driving under unfamiliar traffic regulations, which may lead to a reduction in road safety. The need to adjust to different traffic rules could be a major factor toward a safer drive. Gamification is a promising way to enhance the user engagement in non-game tasks. In this paper, we hypothesize that gamification can improve driving performance and minimize the number of driving errors when driving under unfamiliar traffic regulations and thus enhance road safety. A game was designed to provide gamification elements in a simulated driving environment with unfamiliar traffic regulations where the players were motivated to reach the target with no errors. In the experiments, 14 participants who were not familiar with the designed traffic regulations were asked to drive a car simulator in two scenarios. The first scenario had no gamification elements, whereas the second one included gamification elements. The results indicated that gamification significantly helped the participants to drive in the correct traffic flow with the proper use of vehicle configuration. Our findings show that gamified simulation is a reasonable method to adjust the required driving performance and behavior to safely drive under unfamiliar traffic regulations.