bluuebunny/crossref_metadata_2025 huggingface dataset:
Glycyrrhizin Alleviates the Damage Caused by Zearalenone and Protects the Glandular Stomach of Chickens
Authors: Sun Tong , Wang Fuhan , Qian Man , Wang Jingjing , Guo Mengyao
Published: February 2025

Zearalenone (ZEA) is a kind of mycotoxin that widely contaminates food and feed and poses a threat to poultry farming. As a natural extract, glycyrrhizin acid (GA) has antioxidant, antibacterial, and anti-inflammatory effects. Although studies have revealed the toxic effects of ZEA on the liver, the mechanism by which GA reduces ZEA’s toxic protective glandular stomach remains unclear. In order to study the therapeutic effect of GA on tissue damage caused by ZEA, we conducted in vivo and in vitro experiments to compare the expression of inflammation, oxidative stress, apoptosis, and necrosis. The results showed that ZEA can induce inflammation in tissues and cells, inducing apoptosis and necrosis. In addition, GA can alleviate the toxic effects caused by ZEA and protect cells. Dietary GA significantly increased the antioxidant capacity of glandulae and inhibited the overexpression of NFκB/IκB-α and its mediated inflammatory response. Moreover, GA decreased the expression of pro-apoptotic factors and necrosis factors, thereby alleviating apoptosis and necrosis of chicken glandular stomach cells. At present, the mechanism of ZEA damage to livers and lungs has been confirmed by studies. However, there have been no studies on GA alleviating the damage caused by ZEA to the glandular stomach. Therefore, the purpose of this study was to explore the mechanism of GA alleviating the damage caused by ZEA in the glandular stomach through in vivo and in vitro experimental comparison. The results may provide some reference for the solution of feed contamination.
Oxidative Stress and Reprogramming of Lipid Metabolism in Cancers
Authors: Li Siqi , Yuan Hang , Li Liang , Li Qin , Lin Ping , Li Kai
Published: February 2025

Oxidative stress is a common event involved in cancer pathophysiology, frequently accompanied by unique lipid metabolic reprogramming phenomena. Oxidative stress is caused mainly by an imbalance between the production of reactive oxygen species (ROS) and the antioxidant system in cancer cells. Emerging evidence has reported that oxidative stress regulates the expression and activity of lipid metabolism-related enzymes, leading to the alteration of cellular lipid metabolism; this involves a significant increase in fatty acid synthesis and a shift in the way in which lipids are taken up and utilized. The dysregulation of lipid metabolism provides abundant intermediates to synthesize biological macromolecules for the rapid proliferation of cancer cells; moreover, it contributes to the maintenance of intracellular redox homeostasis by producing a variety of reducing agents. Moreover, lipid derivatives and metabolites play critical roles in signal transduction within cancer cells and in the tumor microenvironment that evades immune destruction and facilitates tumor invasion and metastasis. These findings suggest a close relationship between oxidative stress and lipid metabolism during the malignant progression of cancers. This review focuses on the crosstalk between the redox system and lipid metabolic reprogramming, which provides an in-depth insight into the modulation of ROS on lipid metabolic reprogramming in cancers and discusses potential strategies for targeting lipid metabolism for cancer therapy.
Morphological Analysis of US Treated PANC-1 Spheroids
Authors: Ricci Martina , Dimitri Mattia , Serio Martina , Corvi Andrea
Published: February 2025

This study investigates the impact of low-intensity continuous ultrasound (LICU) on pancreatic adenocarcinoma (PANC-1) spheroids, emphasizing morphological and volumetric transformations. PANC-1 spheroids were cultured and treated with LICU across frequencies from 1 to 5 MHz. Cell viability and mortality were analyzed through Calcein AM/PI staining, while volumetric and morphological changes were quantified across frequencies from 2 to 4 MHz using advanced imaging techniques and computational tools, including a custom Python OpenCv Library, AnaSP 3.0, a MATLAB based open source tool. Notably, a frequency of 3.5 MHz yielded optimal outcomes, also achieving a reduction in spheroid volume and mortality while minimizing disgregation, a factor linked to metastasis risk. These findings underscore LICU’s potential as an effective therapeutic strategy, balancing tumor reduction with the preservation of structural cohesion. The study establishes a methodological framework for optimizing LICU parameters, presenting a less invasive avenue for improving therapeutic outcomes in pancreatic cancer treatment.
Multicriteria Methodology for Evaluating Energy Management Strategies in Heavy-Duty Fuel Cell Electric Vehicles via Vehicular Models
Authors: Arribas Jaime Rodriguez , Nájera Jorge , Alcalá Enrique , Segale Gabriele , Álvarez Jaime
Published: February 2025

In this paper, a methodology for selecting the Energy Management Strategy (EMS) that best suits a heavy-duty Fuel Cell Electric Vehicle (FCEV) operating under specific conditions along a given driving cycle is proposed. Using a simulation model that incorporates the powertrain architecture and components of a specific FCEV—validated through a more detailed model operating at the power converter switching level—the performance of the entire system can be tested under different EMSs. The multicriteria evaluation system developed in this study enables the calculation of hydrogen and energy consumption, as well as the aging of the battery and fuel cell associated with each EMS. The proposed methodology serves as an evaluation tool for both the dimensioning of powertrain components and the selection of the EMS that best meets the operational requirements of a given FCEV. Results demonstrate that applying this methodology to a use case tailored for commercial devices and a standard driving cycle enables the identification of the most suitable EMS, minimizing hydrogen and energy consumption while reducing battery and fuel cell aging.
Calculation of the Main Parameters of the Two-Line Helical Traction Transmission of an Electric Locomotive Based on Diagnostic Parameters
Authors: Khromova Galina , Radjibaev Davran , Zabiyeva Aliya , Kenesbek Anuar , Mavlanov Adham
Published: February 2025

Gearboxes used in electric locomotives are a critical unit, especially in freight rolling stock. The article presents the calculation of the main parameters of the two-way oblique traction transmission of the VL-80s electric locomotive (which is operated on the railways of Uzbekistan) based on a comprehensive analysis of the diagnostic parameters obtained using the Poisson normal distribution method for the identified failures according to the Uzbekistan depot data. Also, a Pareto diagram was constructed for the chassis of 3VL-80s electric locomotives based on the data of the Locomotive Operation Department of JSC Uzbekistan Temir Yollari, and probabilistic and statistical analyses of the failures and breakdowns of the wheel pair and the large gear wheel of the traction gearbox of the VL-80s electric locomotives were carried out. An algorithm and methodology for assessing the reliability of the large gear wheel of the traction gearbox of an electric locomotive are presented. As a result of a numerical calculation of the coefficients of the empirical regression equations using approximation and spline interpolation methods, a regression equation was obtained for the dependence of the standard deviation of the wear of the tooth of the large gear wheel of the traction reducer of the VL80s electric locomotive on the mileage.
Study on the Effect of Plant Growth on the Power Generation Performance of CdTe Photovoltaic Glass Curtain Walls
Authors: Mu Dawei , Yang Xiaoyong , Zhang Yixian
Published: February 2025

The high summer temperatures of PV (photovoltaic) glass curtain walls lead to reduced power generation performance of PV modules and increased indoor temperatures. To address this issue, this study constructed a test platform for planted photovoltaic glass curtain walls to investigate the effect of plants on their power generation performance. The study’s results indicate the following: (1) reducing the average surface temperature of the surface temperature measurement instrument for the photovoltaic glass curtain wall by 13.6 °C can increase its average power generation capacity by 76 w, demonstrating its power generation performance; (2) plant cultivation influences the micro-environmental temperature on the surface temperature of the photovoltaic glass curtain wall, resulting in a decrease in average micro-environmental temperature by 3.2 °C and average surface temperature by 10.1 °C; (3) compared to traditional PV glass curtain walls, the planted PV glass curtain wall increases cumulative PV power generation output by 21.5 kWh over 15 days and average daily power generation output by 1.4 kWh. Furthermore, during sunny weather with high temperatures, the PV power generation output of the planted PV glass curtain wall is significantly enhanced.
Relationship of Individual Task-Specific Functional Brain Connectivity with Sex Differences in Developmental Dyslexia
Authors: Taskov Tihomir , Dushanova Juliana
Published: February 2025

Previous EEG studies using graph analysis have revealed altered functional brain networks in children with developmental dyslexia (DD). The influence of sex on these networks within this childhood disorder remains unclear. The study emphasizes the importance of considering sex and individual differences by investigating brain connectivity in 8-year-old children (42 controls and 72 children with DD, half girls) during a task involving low- and high-contrast discrimination of low-spatial frequency illusion (LSFI). Understanding these variations is crucial for elucidating the neurobiological underpinnings of developmental disabilities. Control children showed sex differences in association networks, while children with DD exhibited them in sensorimotor networks. The control boys’ α, β2-frequency functional networks were more integrated than control girls in low-contrast LSFI and in β and γ2-networks in high-contrast LSFI. Boys exhibited stronger anterior connectivity (language, visual motion), while girls showed stronger posterior connectivity (visuospatial, visuomotor attention). There was a notable overlap in association networks between boys and girls. Sex-related differences were pronounced in the γ2 frequency sensorimotor, and association cortical networks exhibited dispersion in both hemispheres for boys and in the left hemisphere for girls (both contrast LSFIs). Boys with DD exhibited hubs in α-sensorimotor networks (low-contrast LSFI) and β1-networks (high-contrast LSFI) in the right brain hemisphere, while girls’ hubs with DD were in the left hemisphere. The differing rates of cortical network maturation between sexes with DD during childhood contribute to variations linked to disruptions in brain network development, even within sensorimotor networks. The study showed that this task enhanced even minor individual differences in functional connectivity characteristics and revealed subtle differences in brain connectivity, especially in children with DD.
The Interlaced Arches and the So-Called sebka Decoration: Origin and Materialisation in al-Andalus and Its Reinterpretation in Medieval Castile
Authors: González Cavero Ignacio
Published: February 2025

In this article, I aim to address one of the most characteristic decorative elements of the Almohad period, the so-called sebka decoration. With this aim in mind and through the research carried out and the examples that have been preserved, I consider it appropriate to know the origin of this ornamental motif that is so recurrent in the Andalusian architectural panorama and to analyse not only its compositional scheme but also the different formal variants that arose around it. Furthermore, its use in other buildings in the Kingdom of Castile is a further indication that allows us to approach a scenario where cultural and artistic transfer between al-Andalus and the Christian territories was a reality.
Acid Sphingomyelinase and Ceramide Signaling Pathway Mediates Nicotine-Induced NLRP3 Inflammasome Activation and Podocyte Injury
Authors: Rahman Mohammad , Datta Sayantap , Lakkakula Harini , Koka Saisudha , Boini Krishna
Published: February 2025

Background: Recent studies have shown that Nlrp3 inflammasome activation is importantly involved in podocyte dysfunction induced by nicotine. The present study was designed to test whether acid sphingomyelinase (Asm) and ceramide signaling play a role in mediating nicotine-induced Nlrp3 inflammasome activation and subsequent podocyte damage. Methods and Results: Nicotine treatment significantly increased the Asm expression and ceramide production compared to control cells. However, prior treatment with amitriptyline, an Asm inhibitor significantly attenuated the nicotine-induced Asm expression and ceramide production. Confocal microscopic and biochemical analyses showed that nicotine treatment increased the colocalization of NLRP3 with Asc, Nlrp3 vs. caspase-1, IL-1β production, caspase-1 activity, and desmin expression in podocytes compared to control cells. Pretreatment with amitriptyline abolished the nicotine-induced colocalization of NLRP3 with Asc, Nlrp3 with caspase-1, IL-1β production, caspase-1 activity and desmin expression. Immunofluorescence analyses showed that nicotine treatment significantly decreased the podocin expression compared to control cells. However, prior treatment with amitriptyline attenuated the nicotine-induced podocin reduction. In addition, nicotine treatment significantly increased the cell permeability, O2 production, and apoptosis compared to control cells. However, prior treatment with amitriptyline significantly attenuated the nicotine-induced cell permeability, O2 production and apoptosis in podocytes. Conclusions: Asm is one of the important mediators of nicotine-induced inflammasome activation and podocyte injury. Asm may be a therapeutic target for the treatment or prevention of glomerulosclerosis associated with smoking.
Prognostic Factors and Long-Term Outcome Prediction in Patients with Hypopharyngeal Carcinoma Treated with (Chemo)radiotherapy: Development of a Prognostic Model
Authors: Pala Miloslav , Novakova Pavla , Tesar Adam , Vesela Lucie , Vrana Antonin , Sukova Jarmila , Pechacova Zdenka , Holeckova Petra , Drbohlavova Tereza , Podlesak Tomas , Tesarova Petra
Published: February 2025

Background/Objectives: To evaluate the effectiveness of curative (chemo)radiotherapy in patients with hypopharyngeal carcinoma and to identify prognostic factors influencing treatment outcomes. Methods: We conducted a retrospective study of 173 consecutive patients, treated with definitive or postoperative (chemo)radiotherapy from 2002 to 2020 [median age 60 years; current/former smokers 95%; UICC stage III/IV 96%]. Radiation therapy was preceded by a radical resection of a primary tumor in 32% of patients. One hundred patients received chemotherapy. Results: The median total dose of radiotherapy achieved was 70 Gy. The five- and ten-year locoregional controls were 63%, and the five- and ten-year distant controls were 77% and 76%, respectively. The five- and ten-year overall survival rates were 24% and 9%, respectively. Conclusions: The results demonstrate the limited effectiveness of curative (chemo)radiotherapy in patients with hypopharyngeal carcinoma with long-term locoregional and distant control of half of the treated patients. The multivariate analysis indicated that initial surgery, chemotherapy, comorbidity score (as assessed by ACE-27), pretreatment tracheostomy, hemoglobin level and initial response to treatment were the strongest prognostic factors in predicting survival. Using these factors, corresponding predictive models were constructed.
Lactate Clearance of the Adsorber Cytosorb® in Critically Ill Patients: A Post-Hoc Analysis of the Cyto-SOLVE Trial
Authors: Wustrow Vassilissa , Gräfe Caroline , Graf Helen , Scheiermann Patrick , Paal Michael , Vogeser Michael , Liebchen Uwe , Scharf Christina
Published: February 2025

Background/Objectives: Patients with shock suffer from hyperlactatemia, which can lead to endothelial dysfunction. The use of the adsorber Cytosorb® (CS) is recommended in these patients as it may contribute to higher lactate clearance and hemodynamic stabilization. However, it is unclear whether CS can directly adsorb lactate and can therefore increase lactate clearance. Methods: The Cyto-SOLVE trial included patients undergoing continuous kidney replacement therapy combined with CS application. Patients with a lactate concentration > 2 mmol/L and measurements of lactate pre- and post-adsorber, as well as measurements in the blood 10 min and 1, 3, 6, and 12 h after initiation were selected. Lactate clearance was calculated using the following formula: bloodflow(mL/min) × concentrationpre−post/concentrationpre. A t-test was used with the collected samples. Changes in the lactate concentration and vasopressor requirement were recorded before initiation and at the end of therapy. Results: Sixty-five lactate concentrations were measured pre- and post-CS application, as well as in patients’ blood, in a total of 14 patients (median age of 52 years, 10 males, median SAPS-II 67). There was no significant change in the lactate concentration pre- and post-CS application (mean pre-CS: 6.7 mmol/L, mean post-CS: 6.9 mmol/L, RR: −0.2, 95% confidence interval (CI): −0.4–0.1, p = 0.13, Cohen’s d: 0.90). The mean lactate clearance was −6 mL/min (standard deviation (SD): 21 mL/min), with no correlation with the initial lactate concentration or blood flow. In contrast, the mean lactate clearance measured using the dialyzer was 39 mL/min (SD: 28 mL/min). When comparing values before and after treatment, no significant change was observed in the lactate blood concentrations (mean of 9.0 vs. 8.5 mmol/L), nor in the requirement for vasopressin (median of 1.9 vs. 1.8 IE/h) or norepinephrine (mean of 2.7 vs. 2.6 mg/h). Conclusions: The adsorber CS cannot directly adsorb lactate, unlike kidney replacement therapy. Therefore, it is not suitable for achieving faster extracorporeal lactate elimination. Understanding the adsorption spectrum is of great relevance and should be considered when using CS in clinical practice.
Peripheral Immune Profiles in Individuals at Genetic Risk of Amyotrophic Lateral Sclerosis and Alzheimer’s Disease
Authors: Deecke Laura , Ohlei Olena , Goldeck David , Homann Jan , Toepfer Sarah , Demuth Ilja , Bertram Lars , Pawelec Graham , Lill Christina M.
Published: February 2025

The immune system plays a crucial role in the pathogenesis of neurodegenerative diseases. Here, we explored whether blood immune cell profiles are already altered in healthy individuals with a genetic predisposition to amyotrophic lateral sclerosis (ALS) or Alzheimer’s disease (AD). Using multicolor flow cytometry, we analyzed 92 immune cell phenotypes in the blood of 448 healthy participants from the Berlin Aging Study II. We calculated polygenic risk scores (PGSs) using genome-wide significant SNPs from recent large genome-wide association studies on ALS and AD. Linear regression analyses were then performed of the immune cell types on the PGSs in both the overall sample and a subgroup of older participants (>60 years). While we did not find any significant associations between immune cell subtypes and ALS and AD PGSs when controlling for the false discovery rate (FDR = 0.05), we observed several nominally significant results (p < 0.05) with consistent effect directions across strata. The strongest association was observed with CD57+ CD8+ early-memory T cells and ALS risk (p = 0.006). Other immune cell subtypes associated with ALS risk included PD-1+ CD8+ and CD57+ CD4+ early-memory T cells, non-classical monocytes, and myeloid dendritic cells. For AD, naïve CD57+ CD8+ T cells and mature NKG2A+ natural killer cells showed nominally significant associations. We did not observe major immune cell changes in individuals at high genetic risk of ALS or AD, suggesting they may arise later in disease progression. Additional studies are required to validate our nominally significant findings.
Predictors of Ventricular Abnormalities in Children with Idiopathic Ventricular Extrasystoles
Authors: Kunigeliene Rita , Kinciniene Odeta , Marinskis Germanas , Usonis Vytautas
Published: February 2025

Background and Objectives: Ventricular extrasystoles, which are the most common arrhythmias in healthy children and adolescents, could be a reliable factor for the prognosis of structural heart diseases. However, extrasystoles arising in hearts with primary myocardial diseases or channelopathies might cause life-threatening events or be associated with arrhythmia-induced cardiomyopathy. The relationship between ventricular extrasystoles and ventricular abnormalities in children remains controversial. The aim of this study was to evaluate prevalence of ventricular abnormalities in children with ventricular extrasystoles. Materials and Methods: This was a retrospective cohort study of pediatric outpatients in Vilnius University Hospital Santaros Clinics because of ventricular extrasystoles. The inclusion criteria were 3–18-year-old children with more than 5% extrasystoles per 24 h. The exclusion criteria were previous diagnoses of congenital heart defects, cardiomyopathies, and channelopathies. We reviewed the results of electrocardiography, cardiac imaging, and cardiogenetic tests. Results: In total, 131 patients (55.7% males) were included from a database of 915 patients, of whom 79.4% ventricular extrasystoles were found incidentally. Ventricular extrasystoles were monomorphic—95.4%, multiform—4.6%, and consecutive—29.8%. Cardiac magnetic resonance imaging was performed on 22.9% of patients with one-third of the pathological findings (ventricular dysfunction and myocardial fibrosis). Ventricular dysfunction was associated with a higher frequency of ventricular extrasystoles, with a median highest frequency of 26.5% per 24 h. Cardiogenetic testing was performed on only five (3.8%) patients, and RyR2 mutation was detected in one. Conclusions: According to our results, ventricular dysfunction was strongly associated with a higher burden of ventricular extrasystoles.
Effects of Y Additions on the Microstructure and Mechanical Properties of CoCr1.7Ni Medium-Entropy Alloys
Authors: Zhou Shaoshuai , Shu Xiaoyong , Hu Linli , Yuan Xunyu , Qiu Panpan , Xu Xiwen
Published: February 2025

In order to improve the room temperature yield strength of X and enhance its engineering applicability, a series of CoCr1.7NiYx (x = 0, 0.01, 0.02, 0.03, 0.04, and 0.1 at.%) medium-entropy alloys were synthesized to investigate the effect of Y addition on the microstructures and mechanical properties of the CoCr1.7Ni-based alloy. The X-ray diffraction results show that the alloys exhibit face-centered cubic (FCC) + body-centered cubic (BCC) + hexagonal close packing (HCP) triphasic structure when the Y is adopted, whereas the CoCr1.7Ni-based alloy has a FCC+BCC biphasic structure. The volume fraction of BCC and HCP phase increased with increasing Y content, which led to alloy grain refinement. As a result, the microhardness and strength of alloys were both enhanced. The addition of Y resulted in dispersion strengthening and solid solution strengthening of CoCr1.7Ni alloy, the appearance of HCP, and an increase in BCC, which improved the room temperature yield strength and hardness of CoCr1.7Ni alloy. In particular, for CoCr1.7NiY0.1 alloy, its microhardness and yield strength, respectively, increased by 98.18% and 260.59% as compared with those of CoCr1.7Ni alloy.
Four New Species of Hesionidae (Annelida, Polychaeta, Phyllodocida) from Eastern Pacific Chemosynthetic Habitats and Reinstatement of Vrijenhoekia
Authors: Kroesche Rachel , Rouse Greg W.
Published: February 2025

Hesionid polychaetes are well known at hydrothermal vents, methane seeps, and unusual habitats such as whale falls. In 1985, Sirsoe grasslei was the first hesionid to be named from a hydrothermal vent. Vrijenhoekia was erected in 2008 for a closely related species from a whale fall. Further species of Sirsoe and Vrijenhoekia were described in the following decade and the genera were recovered as reciprocally monophyletic with DNA data. However, Sirsoe was made a senior synonym of Vrijenhoekia when 10 further new species from the clade were named from off the coast of Brazil. To further evaluate the status of Sirsoe and Vrijenhoekia, we present the first DNA data for the type species of Sirsoe, S. grasslei. Our results allow the reinstatement of Vrijenhoekia and emended diagnoses for Sirsoe and Vrijenhoekia. Four new species, three in Sirsoe and one in Vrijenhoekia, are also described.
Computed Tomographic Analysis of Mandibular Tori and Their Relationship to Remaining Teeth
Authors: Shibaguchi Kai , Morinaga Kenzo , Magori Yuki , Kagawa Toyohiro , Matsuura Takashi
Published: February 2025

Objectives: Mandibular tori (bilateral, asymptomatic, lingual mandibular protuberances) often remain untreated. When considering surgical resection, understanding the shape, size, and position of the tori at the bone level is crucial. However, collecting accurate information regarding these characteristics is challenging in cases where the oral mucosa is prominent on the floor of the mouth. Methods: We conducted retrospective surveys at Fukuoka Dental College Medical and Dental General Hospital using computed tomographic (CT) image analysis software (Simplant Pro 18.0). The specific aims of this study were to evaluate the appearance rate of mandibular tori by morphological type, size, location, and CT values and their relationship with the remaining teeth in 1176 patients. These patients underwent simple mandibular CT tomography. We used t-tests to analyze the data. Results: Approximately 10% of the identified tori were pedunculated and difficult to diagnose through visual inspection alone. In all the age groups > 30 years, patients with mandibular tori had a lower rate of tooth loss and a higher rate of remaining occlusal support than healthy subjects. The mean CT value of the mandibular tori was >1350 Hounsfield units (HU). Conclusions: These findings provide insights into future classification and treatment planning for mandibular tori, including that in regard to mandibular ridge resection and factors that may contribute to mandibular torus development or progression, and validate the use of excised bone tissue as a bone graft material.
Genome-Wide Identification and Expression Analysis of the Phosphate Transporter Gene Family in Zea mays Under Phosphorus Stress
Authors: Wang Yueli , Li Ronglan , Guo Yuanhao , Du Yan , Luo Zhiheng , Guo Yuhang , Würschum Tobias , Liu Wenxin
Published: February 2025

Phosphorus is one of the key limiting factors for maize growth and productivity, and low-phosphorus stress severely restricts crop yield and stability. Enhancing the ability of maize to grow under low-phosphorus stress and improving phosphorus use efficiency (PUE) are crucial for achieving high and stable yields. Phosphate transporter (PHT) family proteins play a crucial role in the absorption, transport, and utilization of phosphorus in plants. In this study, we systematically identified the PHT gene family in maize, followed by the phylogenetic, gene structure, and expression profiles. The results show that these genes are widely distributed across the 10 chromosomes of maize, forming multiple subfamilies, with the PHT1 subfamily having the largest number. Cis-regulatory element analysis revealed that these genes might play key roles in plant stress responses and hormone regulation. Transcriptome analysis under phosphorus-deficient and normal conditions demonstrated developmental stage- and tissue-specific expression patterns, identifying candidate genes, such as ZmPHT1-3, ZmPHT1-4, ZmPHT1-10, and ZmPHO1-H3, involved in phosphorus stress response. This study presents a comprehensive and systematic analysis of the PHT gene family in maize, providing key molecular resources for improving phosphorus use efficiency and breeding phosphorus-efficient maize varieties.
Biocontrol of Verticillium Wilt of Olive Using Rhizophagus irregularis: Insights into Oxidative Stress and Phenolic Pathways
Authors: Boutaj Hanane , Meddich Abdelilah , Wahbi Said , El Alaoui-Talibi Zainab , Douira Allal , Filali-Maltouf Abdelkarim , El Modafar Cherkaoui
Published: February 2025

Verticillium wilt of olive (VWO), caused by Verticillium dahliae (Vd), represents a significant threat to olive cultivation, particularly to the Moroccan cultivar “Picholine Marocaine”, by reducing yield, tree survival, and overall productivity. Current chemical and cultural control strategies provide limited efficacy, highlighting the need for alternative approaches such as biological control. This study investigated the potential of Rhizophagus irregularis (RI) as a biological agent to mitigate VWO over a 10-month period following V. dahliae inoculation. Disease severity, incidence, and defoliation rate as well as oxidant stress markers, antioxidant enzyme activities, and the phenolic pathway were evaluated in a greenhouse experiment. Our results showed that R. irregularis significantly reduced (p < 0.05) disease severity and incidence by about 31% and 26%, respectively, and decreased defoliation rates by 35% in RI-infected plants (RIV). The presence of R. irregularis triggered enhanced antioxidant enzyme activities (e.g., superoxide dismutase, catalase, and polyphenoloxidase), increased lignin deposition, elevated phenylalanine ammonia-lyase activity, and increased total phenol content in olive tissues. These biochemical and structural changes reduced lipid peroxidation, oxidative stress, and physiological damage, resulting in delayed disease progression. Enhanced accumulation of lignin and total phenolic compounds, particularly in aboveground tissues, was observed. This likely reinforced cell walls and enhanced resistance to pathogen infection. This study demonstrates that R. irregularis offers a promising biocontrol agent for combating soil-borne diseases in olives and other susceptible crops by mitigating VWO, boosting antioxidant defenses, and modulating the phenolic pathway with tissue-specific responses.
Plasma Biomarkers for Cerebral Amyloid Angiopathy and Implications for Amyloid-Related Imaging Abnormalities: A Comprehensive Review
Authors: Sin Mo-Kyung , Dage Jeffrey L. , Nho Kwangsik , Dowling N. Maritza , Seyfried Nicholas T. , Bennett David A. , Levey Allan I. , Ahmed Ali
Published: February 2025

Anti-amyloid therapies (AATs) are increasingly being recognized as promising treatment options for Alzheimer’s disease (AD). Amyloid-related imaging abnormalities (ARIAs), small areas of edema and microbleeds in the brain presenting as abnormal signals in MRIs of the brain for patients with AD, are the most common side effects of AATs. While most ARIAs are asymptomatic, they can be associated with symptoms like nausea, headache, confusion, and gait instability and, less commonly, with more serious complications such as seizures and death. Cerebral amyloid angiopathy (CAA) has been found to be a major risk for ARIA development. The identification of sensitive and reliable non-invasive biomarkers for CAA has been an area of AD research over the years, but with the approval of AATs, this area has taken on a new urgency. This comprehensive review highlights several potential biomarkers, such as Aβ40, Aβ40/42, phosphorylated-tau217, neurofilament light chain, glial fibrillary acidic protein, secreted phosphoprotein 1, placental growth factor, triggering receptor expressed on myeloid cells 2, cluster of differentiation 163, proteomics, and microRNA. Identifying and staging CAA even before its consequences can be detected via neuroimaging are critical to allow clinicians to judiciously select appropriate candidates for AATs, stratify monitoring, properly manage therapeutic regimens for those experiencing symptomatic ARIAs, and optimize the treatment to achieve the best outcomes. Future studies can test potential plasma biomarkers in human beings and evaluate predictive values of individual markers for CAA severity.
Pattern Identification in Patients with Functional Dyspepsia Using Brain–Body Bio-Signals: Protocol of a Clinical Trial for AI Algorithm Development
Authors: Koh Won-Joon , Kim Junsuk , Chae Younbyoung , Lee In-Seon , Ko Seok-Jae
Published: February 2025

Background: Functional dyspepsia (FD) is a common functional gastrointestinal disorder characterized by chronic digestive symptoms without identifiable structural abnormalities. FD affects approximately 8–46% of the population, leading to significant socioeconomic burdens due to reduced quality of life and productivity. Traditional medicine utilizes differential diagnosis through comprehensive examinations, which include observing and questioning, abdominal examination, and pulse diagnosis for functional gastrointestinal disorders. However, challenges persist in the standardization and objectivity of diagnostic protocols. Methods: This study aims to develop an artificial intelligence-based algorithm to predict identified patterns in patients with functional dyspepsia by integrating brain–body bio-signals, including brain activity measured by functional near-infrared spectroscopy, pulse wave, skin conductance response, and electrocardiography. We will conduct an observational cross-sectional study comprising 100 patients diagnosed according to the Rome IV criteria, collecting bio-signal data alongside differential diagnoses performed by licensed Korean medicine doctors. The study protocol was reviewed and approved by the Institutional Review Board of Kyung Hee University Hospital at Gangdong on 25 January 2024 (IRB no. KHNMCOH 2023-12-003-003) and was registered in the Korean Clinical Trial Registry (KCT0009275). Results: By creating AI algorithms based on bio-signals and integrating them into clinical practice, the objectivity and reliability of traditional diagnostics are expected to be enhanced. Conclusions: The integration of bio-signal analysis into the diagnostic process for patients with FD will improve clinical practices and support the broader acceptance of traditional-medicine diagnostic processes in healthcare.
Significance of Preoperative Multidisciplinary Assessment with 30-Second Sit-to-Stand and Timed Up-and-Go Tests in Predicting Postoperative Outcomes
Authors: Yücel Mücahid Osman , Sağlam Sönmez , Dalaslan Raşit Emin , Arıcan Mehmet , Karaduman Zekeriya Okan , Akar Bedrettin , Çelik Mücahit , Sav İsmail
Published: February 2025

Background/Objectives: Evaluating basic daily activities like sitting, standing, and walking is crucial for predicting preoperative risks and postoperative recovery. These functional abilities can be assessed through patient history or measured using objective tests. For this purpose, the 30-Second Sit-to-Stand (30STS) Test and Timed Up-and-Go (TUG) Test are frequently used in clinical settings. However, few studies have evaluated their effectiveness in anesthesia and orthopedics. In this study, we aimed to assess the applicability of these tests across clinical disciplines. Methods: A total of 43 patients who underwent total knee arthroplasty (TKA) surgery between January and December 2023 with American Society of Anesthesiologists (ASA) scores of 2–3 were retrospectively evaluated. The 30STS, TUG, and VAS scores were recorded preoperatively and on postoperative days 90–180. Results: The preoperative 30STS and TUG scores showed no statistically significant difference between the ASA 2–3 groups, but the ASA 2 group demonstrated a more pronounced performance improvement in both tests during the first 90 days postoperatively. The correlation tests revealed a strong positive relationship with the TUG Test and a moderate positive relationship with the 30STS and VAS scores. Conclusions: The correlation between the preoperative and postoperative results of the 30STS and TUG Tests suggests that preoperative tests can predict post-operative functional performance. However, the lack of a significant statistical relationship between the preoperative tests and ASA scores indicates that these tests may not be sufficiently useful for assessing the functional capacity. The better test outcomes in the ASA 2 patients indicate that combining these assessments with anesthetic evaluations may improve postoperative functional predictions, thereby promoting a multidisciplinary approach.
Prevalence and Long-Term Prognostic Significance of Advanced Diastolic Dysfunction Among Hospitalized Patients Referred for Echocardiography
Authors: Dadon Ziv , Moriel Mady , Tirhi Abdallah , Abu Salman Amjad , Glikson Michael , Carasso Shemy , Gottlieb Shmuel
Published: February 2025

Background/Objectives: Left ventricular diastolic dysfunction (LVDD) is associated with unfavorable outcomes, and though it is recognized as an important clinical diagnosis, specific quantification and effective management continue to challenge clinicians, representing an unmet need in modern cardiology. Advanced LVDD diagnosis is likely to have a prognostic role among hospitalized patients. The aim of this study was to describe the prevalence and predictors of advanced LVDD among hospitalized patients and its long-term (5-year) prognostic significance on all-cause mortality. Methods: This was a retrospective observational study of consecutive, non-selected hospitalized patients referred for echocardiography at a tertiary care medical center from October 2013 to February 2024. Diastolic function was classified into normal/LVDD grade I vs. advanced LVDD (grades II and III). Results: A total of 5926 participants were included in the analysis, of whom 3229 (54%) were men, with a mean age of 66 ± 2 years. These included 4779 (81%) patients with normal/LVDD grade I and 1147 (19%) with advanced LVDD. Compared to patients with normal/LVDD grade I, those with advanced LVDD were older, were more likely to be men, and had a higher burden of hypertension, diabetes mellitus, congestive heart failure, atrial fibrillation/flutter and renal failure, abnormal laboratory findings, worse echocardiogram parameters, and longer hospital stay. Multivariate analysis revealed that advanced LVDD was independently associated with increasing age, the male sex, significant aortic stenosis, hypertension, and atrial fibrillation. Patients with advanced LVDD vs. normal/LVDD grade I had higher 5-year all-cause mortality rates (plog-rank < 0.001). Multivariate Cox proportional hazards regression model revealed that advanced LVDD was associated with a 24% increase in the 5-year mortality rate (HR = 1.236, 95% CI of 1.008–1.517, p = 0.042). Conclusions: Among hospitalized patients referred for echocardiography, the prevalence of advanced LVDD was 19%, and it was independently associated with age, the male sex and the presence of multiple comorbidities. Moreover, advanced LVDD was identified as an independent predictor of long-term all-cause mortality. Advanced LVDD should be proactively diagnosed among admitted patients at risk for early therapy tailoring.
Amlodipine-Associated Angioedema: An Integrated Pharmacovigilance Assessment Using Disproportionality and Interaction Analysis and Case Reviews
Authors: Sridharan Kannan , Sivaramakrishnan Gowri
Published: February 2025

Background: Amlodipine has recently been incidentally reported with angioedema and is frequently prescribed with renin–angiotensin–aldosterone system inhibitors (RAAS-i) for hypertension management. While RAAS-i drugs are known to cause angioedema, the risk associated with amlodipine alone or in combination with RAAS-i drugs remains unclear. This study aimed to evaluate the association between amlodipine use and angioedema using pharmacovigilance data. Methods: We analyzed adverse event reports from the US FDA Adverse Event Reporting System using both frequentist and Bayesian approaches. Drug–drug interactions were assessed using multiplicative models. Additionally, we conducted a systematic review of published case reports of amlodipine-associated angioedema. Results: Among 29,661,136 reports, 2076 cases of angioedema were identified (1067 with amlodipine alone, 1009 with amlodipine–RAAS-i combinations). Significant safety signals were detected for amlodipine alone and in combination with aliskiren, specific ACE inhibitors (quinapril, benazepril, trandolapril, fosinopril, perindopril), and certain ARBs (candesartan, losartan). No significant interactions were observed between amlodipine and RAAS-i drugs except for the amlodipine–trandolapril combination. A review of published cases demonstrated definite causality in two cases and possible association in others, with most patients presenting with oropharyngeal/facial edema and achieving complete recovery following drug discontinuation and standard therapy. Conclusions: Our findings suggest a potentially increased risk of angioedema with amlodipine, both as monotherapy and in specific RAAS-i combinations. While these results should not discourage appropriate clinical use, they emphasize the importance of monitoring for angioedema, particularly during therapy initiation. The findings from this study need to be validated in prospective studies for further elucidation of the underlying mechanisms.
Understanding the Intricacies of Khat-Associated Cardiovascular Disease
Authors: Naeem Azka , Situt Divya , Shetty Vijay , Shani Jacob , Emeasoba Emmanuel U.
Published: February 2025

Khat, derived from the Catha edulis plant, is widely consumed in East Africa and the Arabian Peninsula, where it holds significant socio-cultural importance. This review examines the multifaceted effects of khat, particularly focusing on its cardiovascular implications. Khat’s active constituents, notably cathinone and cathine, exhibit stimulant and psychoactive properties akin to amphetamines, leading to heightened alertness and euphoria. However, chronic consumption is associated with adverse effects, including cardiovascular diseases such as hypertension, myocardial infarction, and cardiomyopathy. The review highlights the pharmacokinetics of khat, with cathinone being rapidly absorbed and leading to sympathomimetic effects. Studies indicate a correlation between chronic khat use and increased risks of hypertension, acute coronary syndromes, and cardiomyopathy. These cardiovascular conditions are exacerbated by prolonged hemodynamic stress, catecholamine release, and oxidative stress induced by khat’s active compounds. Additionally, khat’s impact extends beyond the cardiovascular system, affecting neurological, reproductive, and gastrointestinal health. Despite its legality in certain regions, khat is classified as a controlled substance in many countries, emphasizing the need for global awareness of its health risks. This review calls for longitudinal studies to elucidate the pathophysiological mechanisms of khat-induced cardiomyopathy and to identify potential biomarkers for its early detection. Furthermore, it advocates for culturally sensitive public health initiatives and clinical guidelines to mitigate the adverse health effects of khat consumption, especially among immigrant populations in developed nations. Recognizing and addressing khat’s cardiovascular implications is crucial for improving patient outcomes and guiding effective clinical practice.
Advances in the Management of Craniopharyngioma: A Narrative Review of Recent Developments and Clinical Strategies
Authors: Javidialsaadi Mousa , Luy Diego D. , Smith Heather L. , Cecia Arba , Yang Seunghyuk Daniel , Germanwala Anand V.
Published: February 2025

Craniopharyngiomas (CPs) are rare intracranial tumors arising from remnants of Rathke’s pouch. Despite their benign histology, CPs present considerable clinical challenges due to their tendency to exert mass effect and adherence to critical neurovascular structures. There remains no clear consensus on the most effective management of CPs. We explore the latest developments in targeted treatment approaches, examining how innovations in surgery, radiation therapy, and emerging therapies are improving outcomes and management for these challenging tumors. In addition to providing our experience, we reviewed previously reported case series and reviews relevant to CPs. Studies show a bimodal age distribution for CP diagnosis, with peak incidence occurring in children aged 5–14 years and in adults aged 50–74 years. Surgical resection is the typical initial treatment for CPs, and adjuncts, including radiation therapy and intracavitary treatments, have been proven effective for tumor control. Additionally, genetic mutations associated with CPs offer an opportunity for novel strategies that address the underlying molecular mechanisms driving tumor growth through targeting the Wnt/β-catenin and/or MAPK/ERK pathways to disrupt the aberrant signaling that promotes tumor proliferation and survival. Survival rates for CPs are generally favorable (five-year survival of 80%), with recent studies showing improved outcomes and higher survival rates in children. CPs remain rare and challenging tumors. Although surgical resection is the main treatment, surgeons must weigh the benefits of achieving a gross total resection with the risks of iatrogenic injury. Adjuncts, including intracavitary and radiation treatments, may assist with subtotal resections and recurrences, as well as approved BRAF inhibitor therapy for the papillary variant. Many improvements in diagnostic and therapeutic methods were made after Dr. Cushing coined the term “craniopharyngioma”. Ongoing experiments, investigations, and advances in radiation techniques and molecular targeted therapies will provide patients with promise for safer and more effective treatments.
Robotic Adrenalectomy and Clevidipine: A New Frontier in Pheochromocytoma Management Preliminary Study
Authors: Paladino Nunzia Cinzia , Guérin Carole , Loundou Anderson , Domato Nancy , Atondeh Cedric , Taïeb David , Sebag Frédéric
Published: February 2025

Background/Objectives: Adrenalectomy for pheochromocytoma presents a risk due to catecholamine discharge, leading to perioperative hemodynamic instability and potential fatality. Recommendations stress surgical caution and care in referral centers. Laparoscopic and robotic adrenalectomy advancements have decreased perioperative risks, with robotic access deemed advantageous for larger tumors. This study aimed to assess if surgical technique and a new clevidipine-based perioperative protocol could improve hemodynamic stability. Methods: All robotic adrenalectomies treated in recent years (50) were included (Group A). A control group of 50 laparoscopic adrenalectomies (Group B) was also included. Results: In Group A, 7 patients had a BMI > 30 (14%), and 20 patients (40%) had pheochromocytomas > 5 cm in size. During surgery, 22 patients (44%) had systolic blood pressure/SBP ≥ 160 mmHg, and 18 patients (36%) had heart rate/HR ≥ 110 bpm. A total of 44 patients (88%) were treated perioperatively with clevidipine, 32 (64%) required amines perioperatively, and 8 (16%) did not require transfer to intensive care. In Group B, 2 patients had BMI > 30 (4%), 12 (24%) had pheochromocytomas > 5 cm in size, 35 (70%) had SBP ≥ 160 mmHg, 16 patients (32%) had HR ≥ 110 bpm, 23 (46%) patients required amines perioperatively, and all were transferred to intensive care after surgery. In addition, 11 (22%) patients were treated with clevidipine. In both groups, MAP < 60 mmHg was equivalent (62% vs. 60%). Conclusions: The clevidipine-based protocol, combined with robotic adrenalectomy, notably for larger tumors, potentially improves perioperative hemodynamic stability, reducing postoperative intensive care needs. This combination could represent an advancement in managing those patients.