bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of the ocean and marine health performances of 18 countries in the G20 countries: An application using the CEBM-based TOPSIS method
Authors: Altıntaş Furkan Fahri
Published: September 2024

This study investigates the critical role of G20 nations in maintaining ocean health, given the significant influence their economic activities have on global maritime ecosystems. Employing the most recent Ocean Health Index (OHI) data (2023) and the CEBM-TOPSIS Multi-Criteria Decision Making (MCDM) method, the research assesses the ocean health performance of 18 countries G20 countries. The CEBM analysis identifies biodiversity, carbon sequestration capacity, fisheries sustainability, water quality, and coastal protection as the most important OHI criteria, respectively. According to the CEBM-TOPSIS method, Russia, Brazil, and France are the top three countries with the highest ocean health performance, while China, India, and South Africa are ranked lowest among the first three countries. Notably, the average performance score indicates that Russia, Brazil, France, the United Kingdom, Australia, Mexico, South Korea, the United States, Germany, Saudi Arabia, and Canada all exceed the average. This suggests a need for improvement among G20 countries with below-average performance to ensure a more substantial contribution to the global economy and interconnected dimensions. Finally, sensitivity, comparison, and simulation analysis validate the CEBM-TOPSIS MCDM method as a reliable tool for evaluating national ocean health performance.
Length-weight relationships and condition factors of Mystus vittatus (Hamilton, 1822) in natural and culture habitat
Authors: Das Purnachandra , Jana Arun , Sit Godhuli , Mandal Basudev , Chanda Angsuman
Published: September 2024

The Asian catfish Mystus vittatus is very popular food and ornamental fish in India. Length- weight relationships and condition factors of this fish are crucial component in fishery managements. The objective of this study is to investigate the relationships between the length, weight, condition factor (K), relative condition factor (Kn) and modified condition factor of Mystus vittatus (Hamilton, 1822). The study is particularly focus on analysing the sex wise variations in growth patterns, productivity, stocks, and conservation in two different habitats, natural habitat and biofloc system. The findings show that the species did not strictly follow the predicted cube law and showed allometric growth patterns in both habitats. The "b" value varies from 2.72 to 3.12, the condition factor (K) ranges from 0.587-1.50, modified condition factor ranges 0.54-2.26 and relative condition factor (Kn) ranges from 0.71-1.65. Natural habitats had the highest mean values for the regression parameter (b), condition factor (K), and relative condition factor (Kn). For male, female, and combined sexes in two different habitats, the R2 values show a statistically significant relationship between weight and length. The correlation analysis indicates some positive and negative significant relationships among sex, length, weight, condition factor, and relative condition factor. Therefore, there is significant relationship between condition factor, relative condition factor length, weight in two different habitats and it varies between male and female within same habitat. Thus, this finding will help to fishery managers for long term strategy development of Mystus vittatus in both natural as well as biofloc system.
Optimization of extraction conditions for obtaining active compounds of Ulva sp.
Authors: Öder Gizem , Bayraktar Oğuz , Bıçakçı Beyza Tutku
Published: September 2024

Ulva sp., a green macroalgae known as sea lettuce, is rich in polysaccharides, proteins, minerals, and bioactive compounds with antimutagenic, anticoagulant, anticancer, anti-inflammatory, antibacterial, and nutraceutical properties. Its abundance along the Aegean Sea coast poses an environmental challenge, as it is often disposed of as waste. However, Ulva sp. holds potential for high-value products in cosmetics and dietary supplements. Optimizing the extraction of its bioactive compounds using response surface methodology involved adjusting ethanol concentration, solid/liquid ratio, and extraction time. Key responses evaluated included yield, total polysaccharides, total protein, total phenol, total antioxidant activity, alpha-glucosidase inhibitory activity, and yeast cell glucose uptake. In this study, extraction yields ranged from 0.86% to 22.47% based on variations in extraction conditions. The highest total protein content was 106.88 mg BSA/g dry extract, while the polysaccharide content was determined to be 15.42%. The highest values for total phenol content and antioxidant capacity were found to be 82.15 mg GAE/g dry extract and 63.63 mg Trolox/g dry extract, respectively. The determination of the total amounts of antioxidants and phenolic compounds in extracts may expand their potential applications. In addition, the potential application of Ulva sp extracts as inhibitors for the treatment of diabetes has been demonstrated through experiments assessing both alpha-glucosidase enzyme inhibition and glucose uptake in yeast cells. The results support an environmentally friendly approach for the utilization of Ulva sp. from waste into valuable antidiabetic products.
Are potential bisphenol-A substitutes really safe for aquatic life? Impact on primary producers
Authors: Benas Koray , Çakal Arslan Özlem
Published: September 2024

Bisphenol A threat to environmental health and human health and has been added to the Candidate List as Very High Concern Substances by the European Chemicals Agency. This led to the replacement of bisphenol A (BPA) with bisphenol analogues, which were considered "safer". However, there are very few scientific studies on the impact of BPA analogues on the environment. In this study, three analogues bisphenol B (BPB), bisphenol A diglycidyl ether (BADGE) and bisphenol F diglycidyl ether (BFDGE) were selected to investigate their ecotoxicological effects on the marine phytoplankton species Phaeodactylum tricornutum, which is representative of primary producers. Phaeodactylum tricornutum was exposed to different concentrations (0.5, 0.8, 1.0, 1.5, 2.0 mg/L) of BPB, BADGE and BFDGE analogues for 72 hours and the toxicity values of three BPA analogues were calculated by OECD 201 algal growth inhibition assay (IC50/EC50). In the light of the data obtained, algal growth inhibition (IC50/EC50) values for marine phytoplankton Phaeodactylum tricornutum were determined as 3.91 mg-BPA/L, 7.83 mg-BPB/L, 5.69 mg-BFDGE/L, 11.71 mg-BADGE/L. The results revealed that BPB, BFDGE and BADGE showed lower toxicity to Phaeodactylum tricornutum compared to BPA algal growth inhibition (3.91 mg-BPA/L). Therefore, it is necessary to share the results of the adverse effects of BPA analogues on aquatic organisms and to conduct ecotoxicological risk assessments.
Entomoneis parikhani sp.nov., a new diatom (Entomoneidaceae, Bacillariophyta) from Aras River (Iran)
Authors: Parikhani Fatemeh
Published: September 2024

The diatom Entomoneis has a series of unique features such as connecting lines with different shapes, sigmoid raphe on the bilobate keel, different curtains, and multiple girdle bands. Most species belonging to this genus are found in marine and saline habitats. In fact, the genus Entomoneis is an epipelic diatom that is found in streams with high salinity and high electrolyte concentration. To date, no specific type of freshwater has been identified for this species. In this study, a new species of freshwater diatom, Entomoneis parikhani, is described based on detailed morphological observations using a scanning electron microscope. The samples were collected the Aras River near Oltan village of Parsabad city in the northwest Iran. A special feature of Entomoneis parikhani is its triple valve. The genus Entomoneis comprises of diatoms with sigmoidal raphe canals and girdle bands. This taxon is similar to Entomoneis annagodhei and Entomoneis tenera, as shown by LM and SEM observations, and there are sufficient morphological differences to separate Entomoneis parikhani. This new species had very small cells, delicate frustules without valve lines, and wide lanceolate valves. In this study, the wide distribution of this taxon in the Aras River has been evaluated.
Monocularism (unilateral anophthalmia) in the Sea Toad (Chaunax abei Le Danois, 1978) from Suruga Bay, Japan
Authors: Şenbahar Ahmet Mert , Eto Akira , Yokota Masashi
Published: September 2024

The first case of monocularism (unilateral anophthalmia) was observed in a single specimen of Chaunax abei (Le Danois, 1978) during the seasonal trawl sampling in Suruga Bay in 2021. This occurrence marks the first reported incident of such an abnormality within the Chaunacid species. The monocularism was evidenced by photographs, and descriptive statistics of morphometric characters were presented.
A systematic review of age, growth and mortality studies in Mediterranean and Black Sea fishes
Authors: Cerim Hasan , Soykan Ozan , Yapıcı Sercan , Reis İsmail , Yılmaz Özgen
Published: September 2024

Age, growth, and mortality studies (AGMS) conducted in the Mediterranean and the Black Sea were reviewed. The main objective of this study was to find out the gaps on unstudied and less studied species. Names of the all fish species were obtained from fishbase. While the “native” and “endemic” species were taken into consideration, species “introduced”, “questionable”, “misidentified” and “error in a name” were excluded from the data set. Fishbase, semanticscholar and googlescholar were used to obtain the species related studies in June 2023. Graphs and tables were created to represent the results. Totally, 185 of 604 species have AGMS. 22 countries have investigated fish age, growth, and mortalities, and the first three countries are Türkiye, Italy, and Greece, respectively. In the Mediterranean and Black Sea, 796 AGMS were found. The top three of these families with the most species are Sparidae (177), Mullidae (87), and Mugilidae (66). Among the studied species, 86.49% of the species (160) are commercially important for fisheries, and 13.51% of the species (25) are non-commercial. 31 of the 160 species encounter existence problems. All accessible studies were used including studies’ references and it was observed that the most of the species (419) in the Mediterranean and Black Sea have no age or growth studies. This study clearly shows the gaps in AGMS in the Mediterranean and Black Sea regions.
A Study of Paramedics Burnout : Q-methodological Approach
Authors: Oh Jin-Hwan , Lee ln-Soo
Published: September 2024

Purpose: This study was done to give a description of types of paramedics' burnout and explore nursing intervention for each type. Methods: This study was a Q-methodological approach. The Q-sample was made up with 28 statements. The P-sample was 28 paramedics who were working at a fire department in 2 regions. The P-sample sorted the Q-sample for burnout. The data was analyzed by using a PC QUANL program. Results: It was classified by 4 types. Recharging pursuers regarded giving chances of recharging as important. Escapers strongly agreed with frustration and quitting his or her job. And, Ennui type was selected because they got fatigued with his or her work due to exhaustion and dissatisfaction. Finally, Submission type was selected because they rated their job value high by doing his or her best. Conclusion: It is necessary to be approached by types of characteristics in order to control paramedics' burnout effectively. Moreover it is needed to develop programs to relieve or prevent stress which causes burnout.
The Effects of Guided Imagery Program on High School Male Students Stress, Stress Coping, and Health Problem
Authors: Chang Soon Bok , Kim So Ya Ja , Oh Kasil , Yoo II Young , Kim Hee Soon , Kim Sunah , Kim Sue , Hong Min Sun , Bae Choon Hee , Kim Min Jeong , Choi Eun kyoung , Kim Min Jeong , Han Na Rae
Published: September 2024

Purpose: This study was to analyze the effects of Guided Imagery Program on stress, stress coping, and health problem in high school students, and to detail applicable ways of Guided Imagery Program. Methods: A study with quasi-experimental design was conducted from March 27, 2006 through April 21, 2006 with male students in the second grade of high school. 34 students in the experimental group (once a day the guided imagery program received group) and 32 control group remained for the final analysis. Data were analyzed using SPSS 12.0 program by x2-test, t-test and paired t-test. Results: There was no significant group difference in stress, stress coping, and health problem. However, upper 30% group of imagery ability showed significant increase stress coping in 4weeks after pre-test compare to the control group. Conclusion: The results indicate that students who have high level imagery ability are more efficient to guided imagery program.
Correlation of Internet Addiction, Peer Attachment, and Coping with School Life among Middle School Students
Authors: Seo Mia
Published: September 2024

Purpose: The problem of adolescents' Internet addiction is continuously increasing in Korea but appropriate prevention and intervention programs have not been developed. The purpose of this study was to explore correlation among Internet addiction, peer attachment, and coping with school life. Methods: Data were collected from 435 adolescents with using structured questionnaire. Korean version of internet addiction self-test scale(KIA), the inventory of parent and peer attachnient(IPPA), and youth coping scale(YCS) were used. Results: 1) The internet addiction rate was 4.0% and potential risk rate for addiction was 3.3%. 2) There was low positive correlation between internet addiction and peer attachment. 3) Internet addiction and coping with school life were negatively correlated. The subvariables of coping with school life such as planning, responsibility, and self-control had negative correlations to internet addiction. Conclusion: This study showed that Internet addiction had positive correlation with peer attachment but negative correlation with coping with school life. The results of this study will have influence over the development of the Internet addiction prevention program and intervention program.
A Study on the Variables Forecasting Elderly Inpatients' Fall Experience
Authors: Kwon In Gak , Kim Kyung Hee
Published: September 2024

Purpose: This study was designed to identify the variables forecasting elderly inpatients' fall experience through a comprehensive analysis of individual, disease, environment and fall risk related factors. Methods: The medical records of 144 elderly inpatients were reviewed. The data collected from January to July 2003. Frequencies, t-test, X2 -test and logistic regression were done using the SPSS program. Results: Individual-related factors for elderly inpatients' fall experience were age. Fall risk related factors for elderly inpatients' fall experience were fall history and depression. Conclusion: The experience of fall among Korean elderly inpatients was associated not only with individual factors, but also with fall risk factors. The findings of this study suggest that broad intervention program should be provided to prevent related to elderly inpatients' fall.
Development of a Health Educational Program for Korean American Elderly
Authors: Bae Jeong Yee
Published: September 2024

Purpose: Adjusting to life in foreign country produces a great deal of physical and mental health problems. User centered health management program may be particularly useful in Korean American elderly. The purpose of this paper is to describe the development of health management program for Korean American elderly using principals of user centered design. Methods: Our design process includes four distinct phases: needs assessment, analysis, design, development/testing/revision, and application release. Results: This program includes an introduction, important of health management in elderly, major health problems in elderly, self-examination, methods of health management, FAQ, family education, helping resources and institutions. Conclusion: The end result of this program will be Korean American elderly with a high degree of usability. The next stage of this project is construction of a web-based health information service system for all of the Korean American elderly. Author believe this program have true potential in helping Korean American elderly who are suf'fering, or at risk, for health problems, particularly because of lack of health information in American.
Comparison to Self Esteem, Family Adaptation, Health Promoting Behaviors, and Symptoms of Stress between Nursing and Other Major University Women Student
Authors: Han Kuem Sun , Kim Guen Myun
Published: September 2024

Purpose: The purpose of this study was to compare the self esteem (SE), health promoting behavior (HPB), family adaptation (FA) and symptoms of stress(SOS) between nursing and other major university student. Methods: Data were collected by questionnaires from 200 university students in Seoul, Korea. The data were analyzed using descriptive statistics, chi-test, and t-test. Results: The amount of smoking of nursing students showed lower than other women students. The score of HPB of nursing students showed higher than other major women student The score of SE, and SOS of nursing students showed lower than other major student. There were significant relationship among SE, HPB, FA, and SOS. Conclusion: This study suggest that SE, HPB, FA are significantly considerate factors in development stress management program among university students.
The Influence of Ego-Identity and Depression on Suicidal Ideation for Korean Adolescents
Authors: Park Hyun Sook
Published: September 2024

Purpose: The purposes of this study were 1) to investigate the influence of ego-identity and depression on suicidal ideation, 2) assess the mediating effect of depression in the relationship between ego-identity and suicidal ideation for adolescents. Methods: The participants were 940 adolescents. Data were collected through self-report questionnaires, which were constructed to include demographic-environmental factors, ego-identity, depression, and suicidal ideation. The data were analyzed using the SPSS program. Results: Ego-identity negatively related to suicidal ideation, while ego-identity negatively related to depression for adolescents. Depression was significantly related to suicidal ideation for adolescents. A hierarchical strategy in multiple regression was completed to assess the mediating effect of depression. The result indicated that depression mediated the effect of ego-identity on suicidal ideation for adolescents. Conclusion: Based on the outcomes of this study, it is necessary to design an intervention program that emphasizes reducing depression in order to decrease adolescents' suicidal ideation.
Ego States, Parenting Stress and Child Rearing Method in Parents of Child Psychiatric Outpatients
Authors: Lee Sook
Published: September 2024

Objectives: Parents of child psychiatric outpatients may have many stresses due to their children's illness.Personality and characteristics of parents and child will influence to the stress and child rearing method of parents.The author investigated various factors having an effect on the parenting stress and child rearing method of parents. Methods: Parents in outpatients interviewed and completed measures assessing ego states, stress and child rearing control style related to parenting by Korean Ego State scale, Parenting stress Visual Analogue Scale, Child rearing Method Scale. Results: Parents shaped N shaped Egogram with top NP. They perceived the above moderate parenting stress and controlled children using by position. We find the differences in the total score of ego states by education level of father, in the parenting stress by sex of parents, diagnosis of children, in the child rearing method by occupation of father, age of mother, diagnosis of children. But we could not the correlation in the total scores of Ego states, Parenting stress, and child rearing method in parents.
The Relation of Anger and Anger Expression to Menstrual Symptoms and Psychosomatic Symptoms in Female Undergraduate Students
Authors: Park Young-Joo , Chang Sung Ok , Han Kuem Sun , Choi Myung Sook , Yoon Ji-Won , Chun Sook Hee , Moon Sohyun
Published: September 2024

Purpose: This descriptive correlational study was designed to investigate the relation of trait anger and anger expression to menstrual symptoms and psychosomatic symptoms in female undergraduate students, and to examine anger expression (anger-in, anger-out, and anger-control) as a moderator in the association between trait anger and outcome variables. Methods: One hundred and seventy-seven female undergraduate students were conveniently sampled from K University and S College located in Seoul, Korea. The instruments were Spielberger's state-trait anger expression inventory - Korean version, Chesney & Tasto's menstrual symptoms questionnaire, and Kim*s personality factor scale for psychosomatic symptoms of adolescence. Results: The severity level of menstrual symptoms and psychosomatic symptoms were higher in the female undergraduate students with relatively high trait anger score. There was a significant interaction between trait anger and anger-in with psychosomatic symptoms.Female undergraduate students were categorized with 'anger-in/out type 5 , ‘low anger expression type’, and 'anger-control type’ by cluster analysis. The anger- in/out type complained more psychosomatic symptoms than the other two types. Conclusions: The finding suggests that trait anger is an independent risk factor for menstrual symptoms and psychosomatic symptoms and anger-in has a moderating effect in the association between trait anger and psychosomatic symptoms.
Psychological Impact and Depressive Response of Sufferers of Natural Disaster, a Torrential Downpour
Authors: Choi Namhee , Byun Joohoon , Han Dong , Im Sook-Bin
Published: September 2024

Purpose: To investigate the psychological impact and depressive response of the sufferers of the natural disaster, a torrential downpour. Methods: The data were collected from 263 residents and 48 officials at P gun in Gangwon province using the questionnaire, IES-R and CES-D at 3 months after the disaster. The data were analyzed using descriptive statistics. Results: Seventy eight percent of the residents and 48% of officials had much higher psychological impact than 25 cutoff score for PTSD problem. And about 70% of residents and 38% of officials were much more depressive than 21 cutoff score. Psychological impact and depression were significantly different according to subjects' age, education, and income. The people who were elderly, lower income, and lower education. The psychological impact was highly correlated positively each other. Conclusion: It is necessary to understand that the sufferers including rescue workers have very high psychological impact and depression after disaster. And the psychiatric nurse should be pro-active as a practitioner or researcher to develop community mental health services, such a crisis intervention or a empowering class to cope with disaster.
The Effects of Self-esteem, Family Support and Perceived Health State on the Stress of Adult Examinee
Authors: Lee Jong Hwa , Kim Hee Young
Published: September 2024

Purpose: The objectives of this study are to identify stress, self-esteem, family support and perceived health state experienced by Adult Examinee. Methods: Data were collected from the 248 subjects preparing for national examinations using the BEPSI-K, the Rosenberg Self-esteem Scale(SES), family support and the perceived health state scale. The data were analyzed, using SPSS/PC Win 10.0. Results: In stress, the subjects registered possible 25points, averaging 12.49. It indicates that on an average the subjects suffer from mild stress. In self-esteem, the subjects registered possible 40 points, averaging 29.92. In family support, possible 55 points, averaging 43.68. and In perceived health state possible 15 points, averaging 8.20. The findings of this study, negative correlation was identified between self-esteem(r=-.390, p<0.01) and stress. The same tendency was identified in the correlation between family support(r=-.237, p<0.01) and stress. By contrast, positive correlation was identified between perceived health state(r=.281, p<0.01) and stress. Variables affecting the stress were self-esteem(P=-.352, p=.000) and perceived health state((P=.235, p=.000). Conclusion: This findings of this study recommended that a variety of self-esteem and perceived health state-related programs should be developed for the Applicants Preparing for National Examinations.
The Effects of Mother-Child Therapeutic Touch Play for Autistic Children
Authors: Ju Se-Jin
Published: September 2024

Purpose: This study was aimed at evaluating the effects of Mother-child Therapeutic Touch Play for the autistic children. The subjects of this study consisted of 32 pairs autistic children and their mothers. Methods: The children were between 30months ~ 53months of age and diagnosed as Autism by Child and Adolescent Psychiatrists. Data were collected by using video-taping methods(ADOS), observational methods(CARS) and questionnaires (demographic data). The data were analyzed with paired t-test. The results of this study were as follows. Results: The autistic symptom which was measured by ADOS showed significant differences(t=5.936, p=.000) after Mother-child therapeutic touch play. There were statistically significant differences in communication range(t=5.416, p=.000) and reciprocal social interaction range(t=5.543, p=.000) in ADOS subscale. Although there was no significant difference in CARS, the sub-group showed significant differences in communication(t=4.676, p=.000), Social interaction(t=5.544, p=.000), Sensory area(t=5.106, p=.000), Activity(t=4.435, p=.000). This study suggests that Mother-child therapeutic touch play might be a effective intervention for the autistic disorder children in order to decrease autistic symptom.
Relationship between Vaccinations and Multiple Sclerosis
Authors: Sievers Eric J , Heyneman Catherine A
Published: September 2024

OBJECTIVE:

To evaluate recent reports of vaccinations causing or exacerbating multiple sclerosis (MS).

DATA SOURCES:

Literature was identified through MEDLINE (1966–December 2001). Key search terms included vaccinations, hepatitis B, influenza, tetanus, and multiple sclerosis.

DATA SYNTHESIS:

Case reports of MS temporally associated with hepatitis B vaccination have prompted some researchers to propose that vaccines may provoke demyelinating diseases. Recently, studies have been performed to determine whether vaccinations are capable of causing or exacerbating MS symptoms.

CONCLUSIONS:

Recent studies reaffirm that vaccinations do not increase the risk of symptom exacerbation in patients with MS or constitute a causative agent in the disease.
Molecular genotyping and subgenotyping of duck circovirus at duck farms in Thailand
Authors: Kulprasertsri Sittinee , Songserm Thaweesak , Phatthanakunanan Sakuna , Saengnual Pattrawut , Sinwat Nuananong , Khamtae Raktiphorn , Lertwatcharasarakul Preeda
Published: September 2024

Background and Aim: Ducks worldwide are infected with duck circovirus (DuCV), which causes feather abnormality, emaciation, and poor growth performance. DuCV is similar to other circoviruses that induce immunosuppression due to the occurrence of the bursae of Fabricius (BF) and spleen atrophies. In Thailand, retarded ducks with feather losses were submitted for disease investigation. The ducks presented low body weight gain, had small BF and spleens, and were consistent with duck-infected DuCV. Our study investigated the possibility of DuCV infection in duck flocks in Thailand. We also analyzed the genetic characteristics of the virus.

Materials and Methods: BF and spleen samples were collected from affected meat and layer ducks from six farms thought to have been infected with DuCV. These tissues were then subjected to histopathological examination and molecular identification using conventional polymerase chain reaction and nucleotide sequencing. To identify DuCV, phylogenetic trees were generated using MEGA version X software. Samples of tissues or swabs were collected to determine whether coinfections with bacteria and viruses existed.

Results: Phylogenetic analysis using the entire genome (1995–1996 bp) and cap gene (762 bp) revealed that the DuCV isolates circulating in Thailand belonged to DuCV genotype I, which was further subdivided into two sub-genotypes: sub-genotype I b and an unclassified sub-genotype based on reference sub-genotypes. Thai isolates have variations in 10 amino acid residues in the capsid protein. Ducks infected with Thai DuCV were also coinfected with Riemerella anatipestifer, Escherichia coli, Pasteurella multocida, duck viral enteritis, and duck Tembusu virus, which is consistent with previous DuCV infection studies.

Conclusion: Six DuCVs from ducks who were previously found to have feather loss, were underweight, had growth retardation, and had poor body condition were identified in this study as belonging to genotype Ⅰ and constituting at least two sub-genotypes. Due to the immunosuppressive effects of DuCV, coinfection of bacterial and viral pathogens was typically observed in Thai DuCV-infected ducks.

Keywords: duck, duck circovirus, genetic characterization, immunosuppression, phylogenetic tree.
Sex identification of sun conure (Aratinga solstitialis) using loop-mediated isothermal amplification of W and Z spindlin chromosomes
Authors: Wancham Parichart , Phatthanakunanan Sakuna , Jala Siriluk , Woramahatthanon Kanyakodchanan , Sripiboon Supaphen , Lertwatcharasarakul Preeda
Published: September 2024

Background and Aim: The sun conure (Aratinga solstitialis), a bird belonging to the Psittaciformes family, is a popular pet because of its bright color and beautiful appearance. The sun conure is a monomorphic bird with similar appearances between males and females, making sex identification difficult by observing the external morphology. Therefore, molecular techniques are utilized. Loop-mediated isothermal amplification (LAMP) is a molecular technique that is often applied for sex identification in birds and is a quick and simple method that can be used in the field. This study used the LAMP technique to improve sex identification in sun conures by observing the color change of hydroxy naphthol blue.

Materials and Methods: Two primer sets, SunSpin-W and SunSpin-Z, were designed for sex identification in sun conures using the LAMP technique specific to the spindlin gene. The developed LAMP reaction was tested for optimal conditions, sensitivity, and specificity compared with the polymerase chain reaction (PCR) technique.

Results: The SunSpin-W primer set amplified only female birds, whereas the SunSpin-Z primer set amplified DNA from both male and female birds. The primer sets were optimized at 62°C for 45 min. A positive result was visible to the naked eye from the color change of the reaction. In the LAMP assay, the lowest detectable concentration was 10 pg/μL and in the PCR assay, it was 1 ng/μL, while a 100% accuracy rate in sex identification was observed when comparing the LAMP assay results with the PCR assay.

Conclusion: This study successfully developed a LAMP technique for sex identification of sun conure, which took 45 min to complete and can be expanded for use in the field.

Keywords: loop-mediated isothermal amplification, sex identification, spindlin gene, sun conure.
Characterizing the bacteriophage PKp-V1 as a potential treatment for ESBL-producing hypervirulent K1 Klebsiella pneumoniae ST258 isolated from veterinary specimens
Authors: Tariq Muhammad Usama , Muzammil Saima , Ashfaq Usman Ali , Arshad Muhammad Imran , Shafique Muhammad , Ejaz Hasan , Khurshid Mohsin , Eltayeb Lienda Bashier , Mazhari Bi Bi Zainab , Elamir Mohammed Yagoub Mohammed , Al-Harthi Helal F. , Rasool Muhammad Hidayat , Aslam Bilal
Published: September 2024

Background and Aim: The dearth of new antibiotics necessitates alternative approaches for managing infections caused by resistant superbugs. This study aimed to evaluate the lytic potential of the purified bacteriophage PKp-V1 against extended-spectrum β-lactamase (ESBL) harboring hypervirulent Klebsiella pneumoniae (hvKp)-K1 recovered from veterinary specimens.

Materials and Methods: A total of 50 samples were collected from various veterinary specimens to isolate K. pneumoniae, followed by antimicrobial susceptibility testing and molecular detection of various virulence and ESBL genes. Multilocus sequence typing of the isolates was performed to identify prevalent sequence types. The bacteriophages were isolated using the double-agar overlay method and characterized using transmission electron microscopy, spot tests, plaque assays, stability tests, and one-step growth curve assays.

Results: Among 17 (34%) confirmed K. pneumoniae isolates, 6 (35%) were hvKp, whereas 13 (76%) isolates belonging to the K1 type were positive for the wzy (K1) virulence gene. All (100%) hvKp isolates exhibited the allelic profile of ST258. Overall, PKp-V1 exhibited an 88 % (15/17; (p ≤ 0.05) host range, among which all (100 %; p ≤ 0.01) hvKp isolates were susceptible to PKp-V1. PKp-V1 exhibited a lytic phage titer of 2.4 × 108 plaque forming unit (PFU)/mL at temperatures ranging from 25°C to 37°C. The lytic phage titers of PKp-V1 at pH = 8 and 0.5% chloroform were 2.1 × 108 PFU/mL and 7.2 × 109 PFU/mL, respectively.

Conclusion: Although the incidence of ESBL-infected K. pneumoniae in veterinary settings is worrisome, PKp-V1 phages showed considerable lytic action against the host bacterium, indicating the potential of PKp-V1 as a possible alternative therapeutic option against MDR K. pneumoniae.

Keywords: antibiotic resistance, bacteriophage, Klebsiella pneumoniae, veterinary.
Extended-spectrum β-lactamase-producing Escherichia coli from poultry: A review
Authors: Widodo Agus , Khairullah Aswin Rafif , Effendi Mustofa Helmi , Moses Ikechukwu Benjamin , Agustin Alfiana Laili Dwi
Published: September 2024

Extended-spectrum β-lactamases (ESBLs) are β-lactamase enzymes produced by Gram-negative bacterial pathogens that harbor the ESBL genes. In addition, most ESBL genes are plasmid-mediated and usually encode a broader spectrum of antimicrobial resistance, especially to penicillins, first-generation, second-generation, and third-generation cephalosporins, as well as monobactam, such as aztreonam. Escherichia coli has become an opportunistic pathogen, especially in poultry, and has been implicated in zoonotic diseases that can be transmitted to humans, resulting in public health problems. Poultry can act as carriers of ESBL-producing E. coli (ESBL-EC) bacteria to humans through poultry meat that is contaminated by waste products, feces, and excretions. The ESBL gene CTX-M type was identified as the main cause of infection in humans and was detected in poultry as a cause of infection accompanied by clinical symptoms. Several studies have also shown a link between E. coli and ESBL gene transfer from birds to humans. Controlling the spread of ESBL-EC involves maintaining the cleanliness of poultry products, especially meat, and eliminating contaminant sources from poultry. Likewise, maintaining the environmental cleanliness of poultry slaughterhouses and poultry farms must be taken as a precautionary measure to curtail the increasing spread of ESBL-EC into the environment. This review aimed to explain the spread of ESBL-producing E. coli in poultry.

Keywords: Escherichia coli, extended-spectrum β-lactamase, poultry, public health, zoonosis.
Historical narratives: how Portuguese students aged 10 and 15 make use of national history
Authors: Moreira Ana Isabel , Barca Isabel
Published: September 2024

The main purpose of this study is to demonstrate how students make use of their knowledge about national history, while considering relevant perspectives in history education about the development of historical consciousness. A total of 103 students attending the sixth or the ninth grade in Portuguese schools were asked to ‘spontaneously’ write about the history of Portugal. This allowed the understanding of their historical literacy and the identification of the development of their historical consciousness. The data analysis was mainly based on a qualitative approach inspired by the grounded theory, carried out through a process of coding of the narrative elements in order to find meanings on conceptual trends. Generally, the results suggest the prevalence of similar schematic templates in the students’ narratives. At the same time, the research process highlighted two main categories: the assumption of a certain national identity and the understanding of intertemporal relations for personal orientation. Within the former, students either appear tied to fixed conceptions of the past, more often in the sixth grade, or to present a more historically balanced pattern, although still somewhat ethnocentric. In the latter, the idea of connecting the past and the present appears to be the overall trend, particularly in the ninth grade.
Telaah Kualitas Hidup Pasien Penyakit Kronik di Kalimantan Barat
Authors: Untari Eka Kartika , Yuswar Muhammad Akib , Rizkifani Shoma
Published: September 2024

Background: The long duration of chronic disease affecting the patient’s quality of life. Every disease including chronic disease has a different duration and prognosis. The instrument that being used in measuring the quality of life can be very important factor. Quality of life assessment has a benefit by evaluate the treatment of patients with chronic disease, moreover the good quality of life will also increase the patient's life expectancy. The purpose of this article are to describe and identify instruments that being used to measure quality of life patients with chronic disease in West Kalimantan.Methods: This study conduct to review the original research articles with the keywords "quality of life", "chronic disease", and "West Kalimantan". Twenty three research articles were obtained in Google Scholar and met the inclusion criteria. The generic instruments such as WHOQOL-BREF and EQ5D are the most widely used to measure the patient’s quality of life.Result: The distribution of chronic diseases that have been assessed were hypertension, chronic renal failure, diabetes mellitus, COPD, pulmonary tuberculosis, asthma, myocardial infarction, stroke, CHD, osteoarthritis, HIV / AIDS, and psoriasis. The disease with the most impact on quality of life is hypertension (6 of 23 articles). There were 53.07% patients has a good quality of life; 15.97% patients has a moderate quality of life; and 30.96% patients has a bad quality of life.Conclusion : Most of the hypertensive patients has a good quality of life compared to other disease groups. Health interventions were needed to improve the quality of life for patients with chronic diseases.
Hubungan Antara Tingkat Pengetahuan dan Perilaku Tentang Katarak Diabetika Penderita Diabetes Mellitus dengan Kejadian Katarak di Puskesmas Gunung Pati Semarang
Authors: Dhaniswara Andhika Adristia , Saubig Arnila Novitasari , Pramono Dodik , Nugraheni Arwinda
Published: September 2024

Background: Cataract is a degenerative disease that causes second highest visual impairment and the highest cause of blindness in the world which is multifactorial. The behavior and knowledge of people with diabetes mellitus in controlling their disease is important considering that diabetes mellitus is one of the significant factors causing cataracts especially if not treated properly.Methods: This research is an observational analytical with cross-sectional approach. The research data was obtained using consecutive sampling from patients with diabetes mellitus who come to the Gunungpati public health center. Research subjects used in this research were 53 patients who fulfill the inclusion and exclusion criteria, the data then analyzed using Chi-square test.Result: There is a significant relationship between the patient’s level of knowledge (p=0,004;OR=5,56;CI=1,67-17,54) and behavior (p=0,04;OR=3,19;CI=1,04-9,80) on the incidence of cataracts.Conclusion : There is a significant relationship between level of knowledge and behavior of people with diabetes mellitus regarding diabetic cataracts on the incidence of cataracts.
Risk Factors Affecting The Incident of Malaria in The Working Area of The Malaimsimsa Public Health Center Sorong City
Authors: Yunus Elga , Haryatmi Dwi
Published: September 2024

Background: Malaria is an infectious disease caused by Plasmodium sp, a single-celled living creature that belongs to the group of the genus Protozoa which is parasitic. The purpose of this study was to determine the influence of environmental factors and behavioral factors on the incidence of malaria in the working area of the Malaimsimsa Community Health Center, Sorong City.Methods: This research is an observational study using a case-control study . The case group in this study included people with malaria as indicated by positive malaria blood test results. Examination of malaria is carried out by means of microscopic examination by making thick and thin blood preparations, stained using Giemsa stain, then identified using a microscope. The control group includes people who are not sick with negative blood test results.Result: The results of this study indicate that all malaria infections that occur are caused by infection with the type of Plasmodium vivax. The risk factors that influence the incidence of malaria are behavioral factors, namely the use of body armor and the use of insect repellent. While the risk factors that do not affect the incidence of malaria are the presence of puddles, temperature and use of mosquito nets.Conclusion : From the results of this study it can be concluded that the factors of stagnant water, temperature and use of mosquito nets are not related to the incidence of malaria, on the contrary the use of body armor and the use of insect repellent have a relationship with the incidence of malaria.
Antibiotic Practices and The Rate of Surgical Site Infection Following Cesarean Section
Authors: Hussein Maiga Ayub , Sutiningsih Dwi , Saidi Ntambi , Frida Chebet , Adi Mateus Sakundarno
Published: September 2024

Background: Cesarean section (CS) is the most performed operation globally. It’s a clean-contaminated surgery with higher surgical site infections (SSIs) risk. The mortality rate associated with cesarean section in Africa is 50 times greater than in high income settings, 11 women die per 1000 CS in sub-Saharan Africa and 14% of women suffer complication (SSIs). Proper antibiotic use can reduce this burden however effective antibiotics are running out due to inappropriate use. This study aimed to assess the pre-cesarean and post-cesarean antibiotic practices and the rate of SSIs following CS at Mbale regional referral hospital (MRRH)Methods: The study was a retrospective and descriptive cross-sectional on CS mothers between 1st January 2020 and 31st December 2022 at MRRH.  Out of the 2432 patient files reviewed, an equal number (104) of women who developed SSIs and those that did not develop SSIs (104) were selected by simple random sampling, total sample size of 208.Result: More than half (n=148; 71.2 %.) received antibiotic prophylaxis. Majority received I.V ceftriaxone (38.0%) within 15minutes (mean duration) before skin incision. 35.1% of 208 that received antibiotic prophylaxis developed SSIs. I.V Metronidazole and Ceftriaxone were the most administered after CS and Oral metronidazole was the most prescribed upon discharge.Conclusion : The rate of SSIs at MRRH was 3% similar to that of Mbarara regional referral hospital at 3.5%. Lack of antibiotics prophylaxis and poor antibiotic prophylaxix timing could explain this rateu underscoring compliance to International, National and Hospital surgical bundles.
Kajian Perilaku Kepatuhan Medikasi Pasien Tuberculosis Paru di Kabupaten Ngawi: Sebuah Model Matematis
Authors: Kartini Puri Ratna , Norachuriya Zedny , Novitasari Karisma Surya , Shintawati Shefina , Wisnubroto Agung Praboow
Published: September 2024

Background: Tuberculosis (TB)  is currently still a health issue in Indonesia. Indonesia is the country that contributes the second largest number of tuberculosis cases after India. Ngawi is one of the cities in East Java Province with a large number of tuberculosis cases. In 2022, tuberculosis cases in Ngawi Regency will double from the previous year with the death rate also increasing from the previous year. This shows that tuberculosis control in Ngawi Regency has not been effective. Efforts are needed to increase medication compliance in tuberculosis sufferers so that therapeutic success can be achieved so that the death rate and tuberculosis transmission rate can be reduced.Methods: This research is an analytical observational study with a cross sectional research design which aims to create a mathematical model of medication adherence in pulmonary TB sufferers in Ngawi Regency. This research involved 102 pulmonary TB sufferers at Dr. Soeroto Hospital, Widodo Hospital and Ngawi Regency Health Center. Pulmonary TB sufferers were selected using a purposive sampling technique. Data were analyzed using multiple logistic regression tests.Result: Based on interviews conducted with 102 respondents, the results obtained were that the variables age, length of treatment, motivation to recover, self-perception, knowledge, access to health facilities, medication assistance, family support, drug side effects and health facility administration were candidates for bivariate testing.Conclusion : The variables of self-perception, knowledge, family support and health facility administration are the dominant factors influencing medication compliance in pulmonary TB sufferers in Ngawi Regency in 2023.
Analisis Hubungan Antara Kualitas Air Bersih dengan Kejadian Penyakit Diare di Wilyah Kerja Puskesmas Tobeo Utara Kabupaten Halmahera Utara
Authors: Soamole Sudirman Diman , Dewi Evi Surtika
Published: September 2024

Background: Diarrhea is one of the diseases based on the environment, poor sanitation such as minimal access to clean water, the existence of poor human feces disposal and poor personal hygiene greatly affect the increase in diarrhea cases. Diarrhea itself in the North Tobelo Health Center is 1,090 cases. This study aims to determine the relationship between well construction, well distance and clean water quality to the incidence of diarrhea.Methods: The sample in this study was 88 respondents. The sampling technique used was simple random sampling..Result: The results of this study indicate that there is a significant relationship between well construction and clean water quality and the incidence of diarrhea (p-value 0.001), there is a significant relationship between well distance and clean water quality and the incidence of diarrhea (p-value 0.004), there is a significant relationship between clean water quality and the incidence of diarrhea (p-value 0.004)Conclusion : There is a significant relationship between well construction, the distance of the well from the source of pollution and the quality of clean water that does not meet the requirements.
Pengaruh Pengalaman Kerja, Tingkat Pendidikan, Dan Disiplin Kerja Terhadap Kinerja Karyawan Pada Perusahaan Wuryani Konveksi di Kota Grobogan
Authors: Widyakto Adhi , Syafitri Laela Deasy , Niati Asih , Ariefiantoro Teguh
Published: September 2024

This study aims to examine and analyze the effect of work experience, education level, and work discipline on employee performance at the Wuryani Convection company in Grobogan City.The population in this study were 30 employees of Wuryani Convection in Grobogan City. The instrument used in this study was in the form of a questionnaire, by testing it using multiple linear regression analysis. Data analysis methods in this study include validity test, reliability test, normality test, multicollinearity test, heteroscedasticity test, multiple linear regression analysis, hypothesis t test, and coefficient of determination.The results of this study indicate that work experience has a positive but not significant effect on employee performance. The level of education has a positive and significant effect on employee performance. And work discipline has a positive and significant effect on employee performance