bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Pathological Method and Clearance Definition for the Evaluation of Margin Status after Pancreatoduodenectomy for Periampullary Cancer. Results of a Multicenter Prospective Randomized Trial
Authors: Nappo Gennaro , Borzomati Domenico , Zerbi Alessandro , Spaggiari Paola , Boggi Ugo , Campani Daniela , Mrowiec Sławomir , Liszka Łukasz , Coppola Alessandro , Amato Michela , Petitti Tommasangelo , Vistoli Fabio , Montorsi Marco , Perrone Giuseppe , Coppola Roberto , Caputo Damiano
Published: April 2021

Background: There is extreme heterogeneity in the available literature on the determination of R1 resection rate after pancreatoduodenectomy (PD); consequently, its prognostic role is still debated. The aims of this multicenter randomized study were to evaluate the effect of sampling and clearance definition in determining R1 rate after PD for periampullary cancer and to assess the prognostic role of R1 resection. Methods: PD specimens were randomized to Leeds Pathology Protocol (LEEPP) (group A) or the conventional method adopted before the study (group B). R1 rate was determined by adopting 0- and 1-mm clearance; the association between R1, local recurrence (LR) and overall survival (OS) was also evaluated. Results. One-hundred-sixty-eight PD specimens were included. With 0 mm clearance, R1 rate was 26.2% and 20.2% for groups A and B, respectively; with 1 mm, R1 rate was 60.7% and 57.1%, respectively (p > 0.05). Only in group A was R1 found to be a significant prognostic factor: at 0 mm, median OS was 36 and 20 months for R0 and R1, respectively, while at 1 mm, median OS was not reached and 30 months. At multivariate analysis, R1 resection was found to be a significant prognostic factor independent of clearance definition only in the case of the adoption of LEEPP. Conclusions. The 1 mm clearance is the most effective factor in determining the R1 rate after PD. However, the pathological method is crucial to accurately evaluate its prognostic role: only R1 resections obtained with the adoption of LEEPP seem to significantly affect prognosis.
Polymetallic Group 4 Complexes: Catalysts for the Ring Opening Polymerisation of rac-Lactide
Authors: Jenkins David T. , Fazekas Eszter , Patterson Samuel B. H. , Rosair Georgina M. , Vilela Filipe , McIntosh Ruaraidh D.
Published: April 2021

Five novel air- and moisture-stable polymetallic Ti and Zr amino acid-derived amine bis(phenolate) (ABP) complexes were synthesised and fully characterised, including X-ray crystallographic studies. The reaction of the ABP proligands with Ti or Zr alkoxides has resulted in the formation of polymetallic aggregates of different nuclearity. The steric bulk on the pendant arm of the ligand was found to play a critical role in establishing the nuclearity of the aggregated complex. Sterically, less-demanding groups, such as H or Me, facilitated the formation of tetrametallic Ti clusters, bridged by carboxylate groups, while increased steric bulk (tBu) led to the formation of binuclear μ-oxo-bridged species. The isolated complexes were employed as catalysts for the ring opening polymerisation (ROP) of rac-lactide. Overall, the Ti catalysts were all active with the smaller, bimetallic Ti aggregates exhibiting relatively faster rates. A monometallic, bis(ABP) Zr complex was found to exert remarkable ROP activity, albeit with limited control over the tacticity and molecular weight distribution of the polymer. A further oxo-bridged Zr cluster was shown to display a previously unprecedented trimetallic structure and achieved a moderate rate in the ROP of rac-lactide.
Tungsten Oxide-Modified SSZ-13 Zeolite as an Efficient Catalyst for Ethylene-To-Propylene Reaction
Authors: Abdullaev Mansurbek Urol ugli , Lee Sungjune , Kim Tae-Wan , Kim Chul-Ung
Published: April 2021

Among the zeolitic catalysts for the ethylene-to-propylene (ETP) reaction, the SSZ-13 zeolite shows the highest catalytic activity based on both its suitable pore architecture and tunable acidity. In this study, in order to improve the propylene selectivity further, the surface of the SSZ-13 zeolite was modified with various amounts of tungsten oxide ranging from 1 wt% to 15 wt% via a simple incipient wetness impregnation method. The prepared catalysts were characterized with several analysis techniques, specifically, powder X-ray diffraction (PXRD), Raman spectroscopy, temperature-programmed reduction of hydrogen (H2-TPR), temperature-programmed desorption of ammonia (NH3-TPD), inductively coupled plasma-atomic emission spectroscopy (ICP-AES), and N2 sorption, and their catalytic activities were investigated in a fixed-bed reactor system. The tungsten oxide-modified SSZ-13 catalysts demonstrated significantly improved propylene selectivity and yield compared to the parent H-SSZ-13 catalyst. For the tungsten oxide loading, 10 wt% loading showed the highest propylene yield of 64.9 wt%, which was 6.5 wt% higher than the pristine H-SSZ-13 catalyst. This can be related to not only the milder and decreased strong acid sites but also the diffusion restriction of bulky byproducts, as supported by scanning transmission electron microscopy-energy dispersive X-ray spectroscopy (STEM-EDS) observation.
Lithium Chloride Protects against Sepsis-Induced Skeletal Muscle Atrophy and Cancer Cachexia
Authors: Lee Ji-Hyung , Kim Seon-Wook , Kim Jun-Hyeong , Kim Hyun-Jun , Um JungIn , Jung Da-Woon , Williams Darren R.
Published: April 2021

Inflammation-mediated skeletal muscle wasting occurs in patients with sepsis and cancer cachexia. Both conditions severely affect patient morbidity and mortality. Lithium chloride has previously been shown to enhance myogenesis and prevent certain forms of muscular dystrophy. However, to our knowledge, the effect of lithium chloride treatment on sepsis-induced muscle atrophy and cancer cachexia has not yet been investigated. In this study, we aimed to examine the effects of lithium chloride using in vitro and in vivo models of cancer cachexia and sepsis. Lithium chloride prevented wasting in myotubes cultured with cancer cell-conditioned media, maintained the expression of the muscle fiber contractile protein, myosin heavy chain 2, and inhibited the upregulation of the E3 ubiquitin ligase, Atrogin-1. In addition, it inhibited the upregulation of inflammation-associated cytokines in macrophages treated with lipopolysaccharide. In the animal model of sepsis, lithium chloride treatment improved body weight, increased muscle mass, preserved the survival of larger fibers, and decreased the expression of muscle-wasting effector genes. In a model of cancer cachexia, lithium chloride increased muscle mass, enhanced muscle strength, and increased fiber cross-sectional area, with no significant effect on tumor mass. These results indicate that lithium chloride exerts therapeutic effects on inflammation-mediated skeletal muscle wasting, such as sepsis-induced muscle atrophy and cancer cachexia.
The Startling Role of Mismatch Repair in Trinucleotide Repeat Expansions
Authors: Richard Guy-Franck
Published: April 2021

Trinucleotide repeats are a peculiar class of microsatellites whose expansions are responsible for approximately 30 human neurological or developmental disorders. The molecular mechanisms responsible for these expansions in humans are not totally understood, but experiments in model systems such as yeast, transgenic mice, and human cells have brought evidence that the mismatch repair machinery is involved in generating these expansions. The present review summarizes, in the first part, the role of mismatch repair in detecting and fixing the DNA strand slippage occurring during microsatellite replication. In the second part, key molecular differences between normal microsatellites and those that show a bias toward expansions are extensively presented. The effect of mismatch repair mutants on microsatellite expansions is detailed in model systems, and in vitro experiments on mismatched DNA substrates are described. Finally, a model presenting the possible roles of the mismatch repair machinery in microsatellite expansions is proposed.
Exercise Training-Induced Extracellular Matrix Protein Adaptation in Locomotor Muscles: A Systematic Review
Authors: Kritikaki Efpraxia , Asterling Rhiannon , Ward Lesley , Padget Kay , Barreiro Esther , C. M. Simoes Davina
Published: April 2021

Exercise training promotes muscle adaptation and remodelling by balancing the processes of anabolism and catabolism; however, the mechanisms by which exercise delays accelerated muscle wasting are not fully understood. Intramuscular extracellular matrix (ECM) proteins are essential to tissue structure and function, as they create a responsive environment for the survival and repair of the muscle fibres. However, their role in muscle adaptation is underappreciated and underinvestigated. The PubMed, COCHRANE, Scopus and CIHNAL databases were systematically searched from inception until February 2021. The inclusion criteria were on ECM adaptation after exercise training in healthy adult population. Evidence from 21 studies on 402 participants demonstrates that exercise training induces muscle remodelling, and this is accompanied by ECM adaptation. All types of exercise interventions promoted a widespread increase in collagens, glycoproteins and proteoglycans ECM transcriptomes in younger and older participants. The ECM controlling mechanisms highlighted here were concerned with myogenic and angiogenic processes during muscle adaptation and remodelling. Further research identifying the mechanisms underlying the link between ECMs and muscle adaptation will support the discovery of novel therapeutic targets and the development of personalised exercise training medicine.
Cooperative Stabilization of Close-Contact Zones Leads to Sensitivity and Selectivity in T-Cell Recognition
Authors: Różycki Bartosz , Weikl Thomas R.
Published: April 2021

T cells are sensitive to 1 to 10 foreign-peptide-MHC complexes among a vast majority of self-peptide-MHC complexes, and discriminate selectively between peptide-MHC complexes that differ not much in their binding affinity to T-cell receptors (TCRs). Quantitative models that aim to explain this sensitivity and selectivity largely focus on single TCR/peptide-MHC complexes, but T cell adhesion involves a multitude of different complexes. In this article, we demonstrate in a three-dimensional computational model of T-cell adhesion that the cooperative stabilization of close-contact zones is sensitive to one to three foreign-peptide-MHC complexes and occurs at a rather sharp threshold affinity of these complexes, which implies selectivity. In these close-contact zones with lateral extensions of hundred to several hundred nanometers, few TCR/foreign-peptide-MHC complexes and many TCR/self-peptide-MHC complexes are segregated from LFA-1/ICAM-1 complexes that form at larger membrane separations. Previous high-resolution microscopy experiments indicate that the sensitivity and selectivity in the formation of closed-contact zones reported here are relevant for T-cell recognition, because the stabilization of close-contact zones by foreign, agonist peptide-MHC complexes precedes T-cell signaling and activation in the experiments.
Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC Signaling
Authors: Windmöller Beatrice A. , Beshay Morris , Helweg Laureen P. , Flottmann Clara , Beermann Miriam , Förster Christine , Wilkens Ludwig , Greiner Johannes F. W. , Kaltschmidt Christian , Kaltschmidt Barbara
Published: April 2021

There is growing evidence that cancer stem cells (CSCs), a small subpopulation of self-renewal cancer cells, are responsible for tumor growth, treatment resistance, and cancer relapse and are thus of enormous clinical interest. Here, we aimed to isolate new CSC-like cells derived from human primary non-small cell lung cancer (NSCLC) specimens and to analyze the influence of different inhibitors of NF-κB and MYC signaling on cell survival. CSC-like cells were established from three squamous cell carcinomas (SCC) and three adenocarcinomas (AC) of the lung and were shown to express common CSC markers such as Prominin-1, CD44-antigen, and Nestin. Further, cells gave rise to spherical cancer organoids. Inhibition of MYC and NF-κB signaling using KJ-Pyr-9, dexamethasone, and pyrrolidinedithiocarbamate resulted in significant reductions in cell survival for SCC- and AC-derived cells. However, inhibition of the protein–protein interaction of MYC/NMYC proto-oncogenes with Myc-associated factor X (MAX) using KJ-Pyr-9 revealed the most promising survival-decreasing effects. Next to the establishment of six novel in vitro models for studying NSCLC-derived CSC-like populations, the presented investigations might provide new insights into potential novel therapies targeting NF-κB/MYC to improve clinical outcomes in NSCLC patients. Nevertheless, the full picture of downstream signaling still remains elusive.
Identification of CTLA-4-Positive Cells in the Human Tonsil
Authors: Tiemann Markus , Atiakshin Dmitri , Samoilova Vera , Buchwalow Igor
Published: April 2021

CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) was originally defined as a T-lymphocyte antigen and was used as a target in cancer immunotherapy. Unfortunately, the existence of CTLA-4 in cells other than T-lymphocytes is often overlooked. The goal of the present study was to analyze the distribution pattern of CTLA-4 in the human tonsils using a panel of anti–CTLA-4 antibodies of different clones. We found that CTLA-4 was expressed in T-lymphocyte cells of various geneses, including hematopoietic cells and their derivatives (monocytes, macrophages, dendritic, plasma cells, mast cells, and neutrophils), as well as stromal cells of mesodermal (mesenchymal) origin and reticular epithelial cells of ectodermal origin. The expression of CTLA-4 in cells of different origins supports the proposition that CTLA-4 is not restricted to the lymphoid cell lineage and can provide broader effects of CTLA-4 on immune regulation.
Efficient Light Trapping from Nanorod-Like Single-Textured Al-Doped ZnO Transparent Conducting Films
Authors: Zhu Jiang , Hu Daqiang , Wang Ying , Tao Chunlei , Jia Hongbao , Zhao Wenping
Published: April 2021

Nanorod-like single-textured Al-doped ZnO (AZO) transparent conducting films were prepared by the simple hydrothermal growth of AZO nanorods on AZO seed layers. The structures, morphologies, optoelectronic properties and light trapping abilities of the AZO films were investigated. The morphological changes of single-textured AZO films depending on growth temperature were shown. Above all, the relation between light trapping abilities and surface morphologies of the single-textured AZO films was studied in detail. The nanorod-like single-textured AZO films prepared at 100 °C exhibited low resistivity, high total transmittance and remarkable enhancement of haze value, which can be acted as transparent electrodes for improving the conversion efficiency of Si-based thin film solar cells.
Stabilization of the Computation of Stability Constants and Species Distributions from Titration Curves
Authors: Schwoebel Stephan Daniel , Höhlich Dominik , Mehner Thomas , Lampke Thomas
Published: April 2021

Thermodynamic equilibria and concentrations in thermodynamic equilibria are of major importance in chemistry, chemical engineering, physical chemistry, medicine etc. due to a vast spectrum of applications. E.g., concentrations in thermodynamic equilibria play a central role for the estimation of drug delivery, the estimation of produced mass of products of chemical reactions, the estimation of deposited metal during electro plating and many more. Species concentrations in thermodynamic equilibrium are determined by the system of reactions and to the reactions’ associated stability constants. In many applications the stability constants and the system of reactions need to be determined. The usual way to determine the stability constants is to evaluate titration curves. In this context, many numerical methods exist. One major task in this context is that the corresponding inverse problems tend to be unstable, i.e., the output is strongly affected by measurement errors, and can output negative stability constants or negative species concentrations. In this work an alternative model for the species distributions in thermodynamic equilibrium, based on the models used for HySS or Hyperquad, and titration curves is presented, which includes the positivity of species concentrations and stability constants intrinsically. Additionally, in this paper a stabilized numerical methodology is presented to treat the corresponding model guaranteeing the convergence of the algorithm. The numerical scheme is validated with clinical numerical examples and the model is validated with a Citric acid–Nickel electrolyte. This paper finds a stable, convergent and efficient methodology to compute stability constants from potentiometric titration curves.
When the Metal Makes the Difference: Template Syntheses of Tridentate and Tetradentate Salen-Type Schiff Base Ligands and Related Complexes
Authors: Mazzoni Rita , Roncaglia Fabrizio , Rigamonti Luca
Published: April 2021

The reaction of organic molecules mediated by a metal center (template synthesis) can result in a final connectivity that may differ from the one obtained in the absence of the metal. The condensation of carbonyl fragments with primary amines form C=N iminic bonds, the so-called Schiff bases, which can act as ligands for the templating metal center by means of the lone pair on the nitrogen atom. This review focuses on the template methods for the reaction between a carbonyl compound (mainly salicylaldehyde) and a primary aliphatic diamine able to prevent the double condensation on both amine groups and obtain tridentate N2O ligands. These adducts, still having one free amino group, can further react, yielding tetradentate salen-type Schiff base ligands. A screening over the transition metals able to show such a template effect will be presented, with particular attention to copper(II), together with their peculiar reactivity and the available crystal structure of the metal complexes and related coordination geometries.
On the Usage of Tapered Undulators in the Measurement of Interference in the Intensity-Dependent Electron Mass Shift
Authors: Valialshchikov Maksim A. , Ruijter Marcel , Rykovanov Sergey G.
Published: April 2021

In nonlinear Thomson scattering, the main emission line and its harmonics form a band-like structure due to the laser pulse shape induced ponderomotive broadening. We propose to use tapered undulators to mimic Thomson scattering and measure the intensity-dependent electron mass shift experimentally. We also numerically show that the effect is observable for realistic electron beams like in DESY or SKIF.
NanoChest-Net: A Simple Convolutional Network for Radiological Studies Classification
Authors: Luján-García Juan Eduardo , Villuendas-Rey Yenny , López-Yáñez Itzamá , Camacho-Nieto Oscar , Yáñez-Márquez Cornelio
Published: April 2021

The new coronavirus disease (COVID-19), pneumonia, tuberculosis, and breast cancer have one thing in common: these diseases can be diagnosed using radiological studies such as X-rays images. With radiological studies and technology, computer-aided diagnosis (CAD) results in a very useful technique to analyze and detect abnormalities using the images generated by X-ray machines. Some deep-learning techniques such as a convolutional neural network (CNN) can help physicians to obtain an effective pre-diagnosis. However, popular CNNs are enormous models and need a huge amount of data to obtain good results. In this paper, we introduce NanoChest-net, which is a small but effective CNN model that can be used to classify among different diseases using images from radiological studies. NanoChest-net proves to be effective in classifying among different diseases such as tuberculosis, pneumonia, and COVID-19. In two of the five datasets used in the experiments, NanoChest-net obtained the best results, while on the remaining datasets our model proved to be as good as baseline models from the state of the art such as the ResNet50, Xception, and DenseNet121. In addition, NanoChest-net is useful to classify radiological studies on the same level as state-of-the-art algorithms with the advantage that it does not require a large number of operations.
Minimal Cardinality Diagnosis in Problems with Multiple Observations
Authors: Kalech Meir , Stern Roni , Lazebnik Ester
Published: April 2021

Model-Based Diagnosis (MBD) is a well-known approach to diagnosis in medical domains. In this approach, the behavior of a system is modeled and used to identify faulty components, i.e., once a symptom of abnormal behavior is observed, an inference algorithm is run on the system model and returns possible explanations. Such explanations are referred to as diagnoses. A diagnosis is an assumption about which set of components are faulty and have caused the abnormal behavior. In this work, we focus on the case where multiple observations are available to the diagnoser, collected at different times, such that some of these observations exhibit symptoms of abnormal behavior. MBD with multiple observations is challenging because some components may fail intermittently, i.e., behave abnormally in one observation and behave normally in another, while other components may fail all the time (non-intermittently). Inspired by recent success in solving classical diagnosis problems using Boolean satisfiability (SAT) solvers, we describe two SAT-based approaches to solve this MBD with multiple observations problem. The first approach compiles the problem to a single SAT formula, and the second approach solves each observation independently and then merges them together. We compare these two approaches experimentally on a standard diagnosis benchmark and analyze their pros and cons.
Clinicopathological Association of Autophagy Related 5 Protein with Prognosis of Colorectal Cancer
Authors: Hu Wan-Hsiang , Yang Wen-Chi , Liu Pei-Feng , Liu Ting-Ting , Morgan Paul , Tsai Wei-Lun , Pan Hung-Wei , Lee Cheng-Hsin , Shu Chih-Wen
Published: April 2021

Gene mutation and pathogenesis bacteria are highly associated with colorectal cancer (CRC) development and progression. Autophagy is a self-clearance pathway to degrade abnormal proteins and infected bacteria in cells. Autophagy plays a dual role in cancer development. Among the autophagy-related (ATG) proteins, ATG5 is the key component required for the core machinery of autophagy. However, the role of ATG5 in CRC malignancy remains unclear. Herein, we found that a high ATG5 protein level was correlated with poor overall survival (OS) and disease-free survival (DFS) of 118 patients with CRC. After stratification with demographic and clinicopathologic factors, a high ATG5 protein level was significantly correlated with unfavorable overall survival in female and elder (>60 year) CRC patients and tumor tissues with poor differentiation, late T stages (III + IV), whereas the ATG5 protein level was positively associated with the overall survival in CRC patients without lymph node invasion and radiation therapy. In contrast, a high ATG5 protein level was significantly associated with worse DFS in CRC patients with early stage of AJCC and no radiation therapy. In addition, colorectal cancer cells stably harboring small interfering RNA (siRNA) against ATG5 diminished the tumorsphere formation and sensitized cancer cells to chemotherapeutic agents. Taken together, our results suggest that ATG5 might be a prognostic biomarker for CRC and a potential therapeutic target for CRC patients.
Quantification of Liver Fibrosis, Steatosis, and Viscosity Using Multiparametric Ultrasound in Patients with Non-Alcoholic Liver Disease: A “Real-Life” Cohort Study
Authors: Popa Alexandru , Bende Felix , Șirli Roxana , Popescu Alina , Bâldea Victor , Lupușoru Raluca , Cotrău Radu , Fofiu Renata , Foncea Camelia , Sporea Ioan
Published: April 2021

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide. This study aimed to evaluate the performance of four ultrasound-based techniques for the non-invasive multiparametric (MPUS) assessment of liver fibrosis (LF), steatosis (HS), and inflammation in patients with NAFLD. We included 215 consecutive adult patients with NAFLD (mean age: 54.9 ± 11.7; 54.5% were male), in whom LF, HS, and viscosity were evaluated in the same session using four new ultrasound-based techniques embedded on the Aixplorer MACH 30 system: ShearWave Elastography (2D-SWE.PLUS), Sound Speed Plane-wave UltraSound (SSp.PLUS), Attenuation Plane-wave UltraSound (Att.PLUS), and Viscosity Plane-wave UltraSound (Vi.PLUS). Transient Elastography (TE) with Controlled Attenuation Parameter (CAP) (FibroScan) were considered as control. All elastographic measurements were performed according to guidelines. Valid liver stiffness measurements (LSM) were obtained in 98.6% of patients by TE, in 95.8% of patients by 2D-SWE.PLUS/Vi.PLUS, and in 98.1% of patients by Att.PLUS/SSp.PLUS, respectively. Therefore, 204 subjects were included in the final analysis. A strong correlation between LSMs by 2D-SWE.PLUS and TE (r = 0.89) was found. The best 2D-SWE.PLUS cut-off value for the presence of significant fibrosis (F ≥ 2) was 7 kPa. Regarding steatosis, SSp.PLUS correlated better than Att.PLUS with CAP values: (r = −0.74) vs. (r = 0.45). The best SSp.PLUS cut-off value for predicting the presence of significant steatosis was 1524 m/s. The multivariate regression analysis showed that Vi.PLUS values were associated with BMI and LSM by 2D-SWE.PLUS. In conclusion, MPUS was useful for assessing fibrosis, steatosis, and inflammation in a single examination in patients with NAFLD.
How Can We Manage Gallbladder Lesions by Transabdominal Ultrasound?
Authors: Okaniwa Shinji
Published: April 2021

The most important role of ultrasound (US) in the management of gallbladder (GB) lesions is to detect lesions earlier and differentiate them from GB carcinoma (GBC). To avoid overlooking lesions, postural changes and high-frequency transducers with magnified images should be employed. GB lesions are divided into polypoid lesions (GPLs) and wall thickening (GWT). For GPLs, classification into pedunculated and sessile types should be done first. This classification is useful not only for the differential diagnosis but also for the depth diagnosis, as pedunculated carcinomas are confined to the mucosa. Both rapid GB wall blood flow (GWBF) and the irregularity of color signal patterns on Doppler imaging, and heterogeneous enhancement in the venous phase on contrast-enhanced ultrasound (CEUS) suggest GBC. Since GWT occurs in various conditions, subdividing into diffuse and focal forms is important. Unlike diffuse GWT, focal GWT is specific for GB and has a higher incidence of GBC. The discontinuity and irregularity of the innermost hyperechoic layer and irregular or disrupted GB wall layer structure suggest GBC. Rapid GWBF is also useful for the diagnosis of wall-thickened type GBC and pancreaticobiliary maljunction. Detailed B-mode evaluation using high-frequency transducers, combined with Doppler imaging and CEUS, enables a more accurate diagnosis.
Attitudes towards the Use of Educational Robotics: Exploring Pre-Service and In-Service Early Childhood Teacher Profiles
Authors: Papadakis Stamatios , Vaiopoulou Julie , Sifaki Eirini , Stamovlasis Dimitrios , Kalogiannakis Michail
Published: April 2021

The introduction of STEM education, and specifically the implementation of educational robotics (ER), has drawn researchers’ attention and has shown that teachers play a crucial role in leading this innovation. The present study concerns in-service and pre-service early childhood teachers, focusing on their perceptions and attitudes about ER use in daily teaching practice. The data were collected via a questionnaire (N = 201) and explored using latent class analysis, which detected distinct clusters/profiles of participants based on their pattern of responses. Two clusters were identified: Cluster1 was relatively homogeneous, including those who share a positive attitude towards ER, while Cluster2 was heterogeneous, comprising participants with inconsistent responses and expressing negative and skeptical thinking. The cluster memberships were associated with external covariates, such as age, years of teaching experience, and variables measuring their technological competencies. The results showed that teaching experience and age were negatively associated with cluster1-membership, while educational robotics knowledge was positively associated. The findings are interpretable, and the implications for education are discussed considering the current literature.
Output Voltage Imbalance Compensation Using dc Offset Voltage for Split dc-Link Capacitor 3-Leg Inverter
Authors: Kim Sun-Pil , Song Sung-Geun , Park Sung-Jun , Kang Feel-soon
Published: April 2021

In this paper, we analyzed the output voltage imbalance and the cause of the offset voltage in 3-phase 3-leg inverters by using Millman’s theory. Based on this result, we proposed a voltage imbalance compensation algorithm using the dc offset voltage that appeared at the neutral point voltage of the load. To apply the proposed imbalance compensation algorithm, it needs a circuit structure of 3-phase 4-wire such as split dc-link capacitor 3-leg inverter and 4-leg inverter. Therefore, the total harmonic distortion (THD) of the load phase current according to the imbalance rate of the load was analyzed for the two inverters. Then, PSIM simulation and experiments based on a 10 kW-prototype of split dc-link capacitor 3-leg inverter were implemented to verify the validity of the proposed imbalance compensation algorithm.
Agent-Based Coordinated Control of Power Electronic Converters in a Microgrid
Authors: Nasri Maryam , Ginn Herbert L. , Moallem Mehrdad
Published: April 2021

This paper presents the implementation of an agent-based architecture suitable for the coordination of power electronic converters in stand-alone microgrids. To this end, a publish-subscribe agent architecture was utilized as a distributed microgrid control platform. Over a distributed hash table (DHT) searching overlay, the publish-subscribe architecture was identified based on a numerical analysis as a scalable agent-based technology for the distributed real-time coordination of power converters in microgrids. The developed framework was set up to deploy power-sharing distributed optimization algorithms while keeping a deterministic time period of a few tens of milliseconds for a system with tens of converters and when multiple events might happen concurrently. Several agents participate in supervisory control to regulate optimum power-sharing for the converters. To test the design, a notional shipboard system, including several converters, was used as a case study. Results of implementing the agent-based publish-subscribe control system using the Java Agent Development Framework (JADE) are presented.
Influence of the Material Composition SMD Diodes on Their Environmental Impact
Authors: Pina Carmelo , Elduque Daniel , Gómez Patricia , Sarasa Judith , Javierre Carlos
Published: April 2021

The influence of the material composition of surface-mount device (SMD) diodes on the environment has been analysed in this research. This impact assessment has been performed by means of an environmental impact calculation through a life cycle assessment (LCA), in which the EcoInvent dataset has been updated and customised, generating a more precise environmental impact analysis by considering the exact material composition provided by several suppliers of diodes and also recycling during the production stage. Considering the EcoInvent diode dataset as a reference, variations from nearly 1640% to only 8.5% of the environmental impact have been achieved. For example, the impact per 1 g of SMD diodes can change the global warming potential from 292 g CO2 eq up to 354 g CO2 eq, whereas for abiotic depletion, values can change from 9.9 × 10−7 up to 1.9 × 10−4 kg Sb eq. The presence of critical raw materials such as antimony, cobalt, or magnesium, together with precious metals as gold or silver, highly influences the environmental impact values obtained, demonstrating the considerable influence on the environmental impact of the material composition of the SMD diodes analysed.
Assessment of Various Window Functions in Spectral Identification of Passive Intermodulation
Authors: Gharaibeh Khaled
Published: April 2021

Passive Intermodulation (PIM) distortion is a major problem in wireless communications which limits cell coverage and data rates. Passive nonlinearities result in weak intermodulation (IMD) products that are very difficult to diagnose, troubleshoot and model. To predict PIM, behavioral models are used where spectral components of PIM are estimated from the power spectral density at the output of the model. The primary goal of this paper is to study the effect of window functions on the capability of the power spectral density computed from the periodogram of signal realizations to predict low power PIM components in a wideband multichannel communication system. Different window functions are analyzed and it is shown that windows with high side-lobe level fail to predict PIM components which are close to the main channels due to their high spectral leakage; while window functions with low roll-off rate of the side lobes fail to predict low power higher order PIM components especially when the frequency separation between the main carriers is high. These results are supported by simulations of the power spectral density computed using signal realizations of an LTE carrier aggregated system.
Spelling Correction Real-Time American Sign Language Alphabet Translation System Based on YOLO Network and LSTM
Authors: Rivera-Acosta Miguel , Ruiz-Varela Juan Manuel , Ortega-Cisneros Susana , Rivera Jorge , Parra-Michel Ramón , Mejia-Alvarez Pedro
Published: April 2021

In this paper, we present a novel approach that aims to solve one of the main challenges in hand gesture recognition tasks in static images, to compensate for the accuracy lost when trained models are used to interpret completely unseen data. The model presented here consists of two main data-processing stages. A deep neural network (DNN) for performing handshape segmentation and classification is used in which multiple architectures and input image sizes were tested and compared to derive the best model in terms of accuracy and processing time. For the experiments presented in this work, the DNN models were trained with 24,000 images of 24 signs from the American Sign Language alphabet and fine-tuned with 5200 images of 26 generated signs. The system was real-time tested with a community of 10 persons, yielding a mean average precision and processing rate of 81.74% and 61.35 frames-per-second, respectively. As a second data-processing stage, a bidirectional long short-term memory neural network was implemented and analyzed for adding spelling correction capability to our system, which scored a training accuracy of 98.07% with a dictionary of 370 words, thus, increasing the robustness in completely unseen data, as shown in our experiments.
A Methodology for Estimating the Position of the Engineering Bedrock for Offshore Wind Farm Seismic Demand in Taiwan
Authors: Kuo Yu-Shu , Weng Tzu-Ling , Hsu Hui-Ting , Chang Hsing-Wei , Lin Yun-Chen , Chang Shang-Chun , Chuang Ya-Jhu , Tseng Yu-Hsiu , Wong Yih-Ting
Published: April 2021

Taiwan lies in the circum-Pacific earthquake zone. The seabed soil of offshore wind farms in Taiwan is mainly composed of loose silty sand and soft, low-plasticity clay. The seismic demand for offshore wind turbines has been given by the local code. Ground-motion analysis is required to consider the site effects of the soil liquefaction potential evaluation and the foundation design of offshore wind turbines. However, the depth of the engineering bedrock for ground motion analysis is not presented in the local code. In this study, we develop a three-dimensional ground model of an offshore wind farm in the Changhua area, through use of collected in situ borehole and PS (P wave (compression) and S (shear) wave velocities) logging test data. The engineering bedrock is the sediment at the depth where the average shear wave velocity of soil within 30 m, Vsd30, is larger than 360 m/s. In this ground model, the shear wave velocity of each type of soil is quantified using the seismic empirical formulation developed in this study. The results indicate that the engineering bedrock lies at least 49.5–83 m beneath the seabed at the Changhua offshore wind farm. Based on these findings, it is recommended that drilling more than 100 m below the seabed be done to obtain shear wave velocity data for a ground response analysis of the seismic force assessment of offshore wind farm foundation designs.
Evidence of Constrained Divergence and Conservatism in Climatic Niches of the Temperate Maples (Acer L.)
Authors: Grossman Jake J.
Published: April 2021

Research highlights: The availability of global distribution data and new, fossil-calibrated phylogenies has made it possible to compare the climatic niches of the temperate maple (Acer L.) taxa and assess phylogenetic and continental patterns in niche overlap. Background and Objectives: The maples have radiated from East Asia into two other temperate continental bioregions, North America and Eurasia (Europe and West Asia), over a roughly 60-million-year period. During this time, the Earth’s climate experienced pronounced cooling and drying, culminating in cyclic periods of widespread temperate glaciation in the Pliocene to Pleistocene. The objective of this study is to use newly available data to model the climatic niches of 60% of the temperate maples and assess patterns of niche divergence, constraint, and conservatism in the genus’s radiation out of East Asia. Materials and Methods: I assembled global occurrence data and associated climatic information for 71 maple taxa, including all species endemic to temperate North America and Eurasia and their closely related East Asian congeners. I constructed Maxent niche models for all taxa and compared the climatic niches of 184 taxa pairs and assessed phylogenetic signal in key niche axes for each taxon and in niche overlap at the continental and global scale. Results: Maxent models define a fundamental climatic niche for temperate maples and suggest that drought-intolerant taxa have been lost from the Eurasian maple flora, with little continental difference in temperature optima or breadth. Niche axes and niche overlap show minimal evidence of phylogenetic signal, suggesting adaptive evolution. Pairwise niche comparisons reveal infrequent niche overlap continentally and globally, even among sister pairs, with few taxa pairs sharing ecological niche space, providing evidence for constrained divergence within the genus’s fundamental climatic niche. Evidence of niche conservatism is limited to three somewhat geographically isolated regions of high maple diversity (western North America, the Caucasus, and Japan). Conclusions: Over 60 million years of hemispheric radiation on a cooling and drying planet, the maple genus experienced divergent, though constrained, climatic niche evolution. High climatic niche diversity across spatial and phylogenetic scales along with very limited niche overlap or conservatism suggests that the radiation of the genus has largely been one of adaptive diversification.
Do Adjacent Forests Affect the Regeneration of Oak-Hornbeam and Ancient Forest Plant Species in Manor Parks in Poland?
Authors: Fornal-Pieniak Beata , Ollik Marcin , Schwerk Axel
Published: April 2021

Manor parks are characteristic objects in the agricultural landscape of Poland. Lack of proper management after World War II, however, led to their devastation from a cultural point of view, but may allow the regeneration of rare and endangered species. The aim of our study was to determine if the presence of forests in the vicinity of manor parks will work as an accelerator of the regeneration process of oak-hornbeam and ancient forest species. Phytosociological analyses were conducted in manor parks adjacent to forests and not adjacent to the forest as well as natural forests. The total number of plant species, number and percentage share of ancient forest species, and plant species consistent with oak-hornbeam habitat were analyzed using a GLM model. Characteristic species were identified using detrended correspondence analysis. Parks adjacent to forests and natural forests showed higher numbers of total species, ancient forest species, and oak-hornbeam species compared with parks not adjacent to forests, but there were no differences in percentage shares of ancient forest species and oak-hornbeam species. For all three types of studied objects, characteristic species could be identified. We conclude that adjacent forests allow greater regeneration of ancient forest species and oak-hornbeam forest species in manor parks.
Effect of Frying and Roasting Processes on the Oxidative Stability of Sunflower Seeds (Helianthus annuus) under Normal and Accelerated Storage Conditions
Authors: Valdés García Arantzazu , Beltrán Sanahuja Ana , Karabagias Ioannis K. , Badeka Anastasia , Kontominas Michael G. , Garrigós María Carmen
Published: April 2021

The effect of different cooking processes such as frying and roasting on the oxidative stability of sunflower seeds was evaluated under accelerated oxidation and normal storage conditions. The fatty acid composition by GC-MS showed a higher amount of linoleic acid in fried samples due to the replacement of the seed moisture by the frying oil. On the other hand, roasted samples presented a higher oleic acid content. DSC and TGA results showed some decrease in the thermal stability of sunflower seed samples, whereas PV and AV showed the formation of primary and secondary products, with increasing oxidation time. Roasted sunflower seeds showed seven main volatile compounds characteristic of the roasting process by HS-SPME-GC-MS: 2-pentylfuran, 2,3-dimethyl-pyrazine, methyl-pyrazine, 2-octanone, 2-ethyl-6-methylpyrazine, trimethyl-pyrazine, and trans,cis-2,4-decadienal, whereas fried samples showed six volatile characteristic compounds of the frying process: butanal, 2-methyl-butanal, 3-methyl-butanal, heptanal, 1-hexanol, and trans,trans-2,4-decadienal. The generation of hydroperoxides, their degradation, and the formation of secondary oxidation products were also investigated by ATR-FTIR analysis. The proposed methodologies in this work could be suitable for monitoring the quality and shelf-life of commercial processed sunflower seeds with storage time.
Application of Spectrometric Technologies in the Monitoring and Control of Foods and Beverages
Authors: Boqué Ricard , Giussani Barbara
Published: April 2021

In order to obtain high-quality products and gain a competitive advantage, food producers seek improved manufacturing processes, particularly when physicochemical and sensory properties add significant value to the product [...]
Development and Evaluation of an Ultrasonic Humidifier to Control Humidity in a Cold Storage Room for Postharvest Quality Management of Dates
Authors: Mohammed Maged , Alqahtani Nashi , El-Shafie Hamadttu
Published: April 2021

Dates are subjected to postharvest losses in quality and quantity caused by water loss, fermentation, insect infestation, and microbial spoilage during storage. Cold storage is the main element in the postharvest quality management used for fruit preservation. Although cold storage is used for dates, precision control of the relative humidity (RH) using ultrasonic applications is not used thus far, or it is applied to other fruits on a small scale. Therefore, we designed and constructed an ultrasonic humidifier (DUH) for RH control in the cold storage room (CSR) of dates. The optimum air velocity of 3 m s−1 at the outlets of the DUH ducts produced a mist amount of 6.8 kg h−1 with an average droplet diameter of 4.26 ± 1.43 µm at the applied voltage of 48 V and frequency of 2600 kHz of the transducers. The experimental validation was carried out by comparing a CSR controlled with the DUH with two conventional CSRs. The three tested CSRs were similar in dimensions, cooling system, and amount of stored dates. The time required for cooling 800 kg of dates in the controlled CSR from 25 °C to the target temperature of 5 °C was approximately 48 h. The DUH precisely controlled the RH at the maximum RH set point of 80% in the tested CSR at 5 °C. The controlled RH at 80% has a positive impact on the physicochemical characteristics of the stored dates. It significantly reduced the weight loss of the fruits and preserved fruit mass, moisture content, water activity, firmness, and color parameters. However, no significant effect was observed on fruit dimensions, sphericity, and aspect ratio. The microbial loads of mesophilic aerobic bacteria, molds, and yeasts fell within the acceptable limits in all tested CSRs. Both stored date fruits and artificially infested dates showed no signs of insect activity in the controlled CSR at the temperature of 5 °C and RH of 80%. The DUH proved to be a promising technology for postharvest quality management for dates during cold storage.
A Proteomic Study for the Discovery of Beef Tenderness Biomarkers and Prediction of Warner–Bratzler Shear Force Measured on Longissimus thoracis Muscles of Young Limousin-Sired Bulls
Authors: Zhu Yao , Gagaoua Mohammed , Mullen Anne Maria , Kelly Alan L. , Sweeney Torres , Cafferky Jamie , Viala Didier , Hamill Ruth M.
Published: April 2021

Beef tenderness is of central importance in determining consumers’ overall liking. To better understand the underlying mechanisms of tenderness and be able to predict it, this study aimed to apply a proteomics approach on the Longissimus thoracis (LT) muscle of young Limousin-sired bulls to identify candidate protein biomarkers. A total of 34 proteins showed differential abundance between the tender and tough groups. These proteins belong to biological pathways related to muscle structure, energy metabolism, heat shock proteins, response to oxidative stress, and apoptosis. Twenty-three putative protein biomarkers or their isoforms had previously been identified as beef tenderness biomarkers, while eleven were novel. Using regression analysis to predict shear force values, MYOZ3 (Myozenin 3), BIN1 (Bridging Integrator-1), and OGN (Mimecan) were the major proteins retained in the regression model, together explaining 79% of the variability. The results of this study confirmed the existing knowledge but also offered new insights enriching the previous biomarkers of tenderness proposed for Longissimus muscle.
Late Pleistocene Expansion of Small Murid Rodents across the Palearctic in Relation to the Past Environmental Changes
Authors: Kozyra Katarzyna , Zając Tomasz M. , Ansorge Hermann , Wierzbicki Heliodor , Moska Magdalena , Stanko Michal , Stopka Pavel
Published: April 2021

We investigated the evolutionary history of the striped field mouse to identify factors that initiated its past demographic changes and to shed light on the causes of its current genetic structure and trans-Eurasian distribution. We sequenced mitochondrial cyt b from 184 individuals, obtained from 35 sites in central Europe and eastern Mongolia. We compared genetic analyses with previously published historical distribution models and data on environmental and climatic changes. The past demographic changes displayed similar population trends in the case of recently expanded clades C1 and C3, with the glacial (MIS 3–4) expansion and postglacial bottleneck preceding the recent expansion initiated in the late Holocene and were related to environmental changes during the upper Pleistocene and Holocene. The past demographic trends of the eastern Asian clade C3 were correlated with changes in sea level and the formation of new land bridges formed by the exposed sea shelf during the glaciations. These data were supported by reconstructed historical distribution models. The results of our genetic analyses, supported by the reconstruction of the historical spatial distributions of the distinct clades, confirm that over time the local populations mixed as a consequence of environmental and climatic changes resulting from cyclical glaciation and the interglacial period during the Pleistocene.