bluuebunny/crossref_metadata_2025 huggingface dataset:
Physical properties ofβLyrae A and its opaque accretion disk
Authors: Mourard D. , Brož M. , Nemravová J. A. , Harmanec P. , Budaj J. , Baron F. , Monnier J. D. , Schaefer G. H. , Schmitt H. , Tallon-Bosc I. , Armstrong J. T. , Baines E. K. , Bonneau D. , Božić H. , Clausse J. M. , Farrington C. , Gies D. , Juryšek J. , Korčáková D. , McAlister H. , Meilland A. , Nardetto N. , Svoboda P. , Šlechta M. , Wolf M. , Zasche P.
Published: July 2018

Mass exchange and mass loss in close binaries can significantly affect their evolution, but a complete self-consistent theory of these processes is still to be developed. Processes such as radiative shielding due to a hot-spot region, or a hydrodynamical interaction of different parts of the gas stream have been studied previously. In order to test the respective predictions, it is necessary to carry out detailed observations of binaries undergoing the largescale mass exchange, especially for those that are in the rapid transfer phase.βLyr A is an archetype of such a system, having a long and rich observational history. Our goal for this first study is to quantitatively estimate the geometry and physical properties of the optically thick components, namely the Roche-lobe filling mass-losing star, and the accretion disk surrounding the mass-gaining star ofβLyr A. A series of continuum visible and NIR spectro-interferometric observations by the NPOI, CHARA/MIRC and VEGA instruments covering the whole orbit ofβLyr A acquired during a two-week campaign in 2013 were complemented withUBVRphotometric observations acquired during a three-year monitoring of the system. We included NUV and FUV observations from OAO A-2, IUE, and Voyager satellites. All these observations were compared to a complex model of the system. It is based on the simple LTE radiative transfer codeSHELLSPEC, which was substantially extended to compute all interferometric observables and to perform both global and local optimization of system parameters. Several shapes of the accretion disk were successfully tested – slab, wedge, and a disk with an exponential vertical profile – and the following properties were consistently found: the radius of the outer rim is 30.0±1.0R⊙, the semithickness of the disk 6.5±1.0R⊙, and the binary orbital inclinationi=93.5±1.0deg. The temperature profile is a power-law or a steady-disk in case of the wedge geometry. The properties of the accretion disk indicate that it cannot be in a vertical hydrostatic equilibrium, which is in accord with the ongoing mass transfer. The hot spot was also detected in the continuum but is interpreted as a hotter part of the accretion disk illuminated by the donor. As a by-product, accurate kinematic and radiative properties ofβLyr B were determined.
XMMPZCAT: A catalogue of photometric redshifts for X-ray sources
Authors: Ruiz A. , Corral A. , Mountrichas G. , Georgantopoulos I.
Published: July 2018

Aims. The third version of the XMM-Newton serendipitous catalogue (3XMM), containing almost half million sources, is now the largest X-ray catalogue. However, its full scientific potential remains untapped due to the lack of distance information (i.e. redshifts) for the majority of its sources. Here we present XMMPZCAT, a catalogue of photometric redshifts (photo-z) for 3XMM sources.

Methods. We searched for optical counterparts of 3XMM-DR6 sources outside the Galactic plane in the SDSS and Pan-STARRS surveys, with the addition of near-(NIR) and mid-infrared (MIR) data whenever possible (2MASS, UKIDSS, VISTA-VHS, and AllWISE). We used this photometry data set in combination with a training sample of 5157 X-ray selected sources and the MLZ-TPZ package, a supervised machine learning algorithm based on decision trees and random forests for the calculation of photo-z.

Results. We have estimated photo-z for 100 178 X-ray sources, about 50% of the total number of 3XMM sources (205 380) in the XMM-Newton fields selected to build this catalogue (4208 out of 9159). The accuracy of our results highly depends on the available photometric data, with a rate of outliers ranging from 4% for sources with data in the optical + NIR + MIR, up to ~40% for sources with only optical data. We also addressed the reliability level of our results by studying the shape of the photo-z probability density distributions.
Bayesian group finder based on marked point processes
Authors: Tempel Elmo , Kruuse Maarja , Kipper Rain , Tuvikene Taavi , Sorce Jenny G. , Stoica Radu S.
Published: July 2018

Context. Galaxy groups and clusters are formidable cosmological probes. They permit the studying of the environmental effects on galaxy formation. A reliable detection of galaxy groups is an open problem and is important for ongoing and future cosmological surveys.

Aims. We propose a probabilistic galaxy group detection algorithm based on marked point processes with interactions.

Methods. The pattern of galaxy groups in a catalogue is seen as a random set of interacting objects. The positions and the interactions of these objects are governed by a probability density. The parameters of the probability density were chosen using a priori knowledge. The estimator of the unknown cluster pattern is given by the configuration of objects maximising the proposed probability density. Adopting the Bayesian framework, the proposed probability density is maximised using a simulated annealing (SA) algorithm. At fixed temperature, the SA algorithm is a Monte Carlo sampler of the probability density. Hence, the method provides “for free” additional information such as the probabilities that a point or two points in the observation domain belong to the cluster pattern, respectively. These supplementary tools allow the construction of tests and techniques to validate and to refine the detection result.

Results. To test the feasibility of the proposed methodology, we applied it to the well-studied 2MRS data set. Compared to previously published Friends-of-Friends (FoF) group finders, the proposed Bayesian group finder gives overall similar results. However for specific applications, like the reconstruction of the local Universe, the details of the grouping algorithms are important.

Conclusions. The proposed Bayesian group finder is tested on a galaxy redshift survey, but more detailed analyses are needed to understand the actual capabilities of the algorithm regarding upcoming cosmological surveys. The presented mathematical framework permits adapting it easily for other data sets (in astronomy and in other fields of sciences). In cosmology, one promising application is the detection of galaxy groups in photometric galaxy redshift surveys, while taking into account the full photometric redshift posteriors.
First observations of magnetic holes deep within the coma of a comet
Authors: Plaschke F. , Karlsson T. , Götz C. , Möstl C. , Richter I. , Volwerk M. , Eriksson A. , Behar E. , Goldstein R.
Published: July 2018

The Rosetta spacecraft of the European Space Agency made ground-breaking observations of comet 67P/Churyumov-Gerasimenko and of its cometary environment. We search for magnetic holes in that environment, i.e., significant depressions in the magnetic field strength, measured by the Rosetta fluxgate Magnetometer (MAG) in April and May 2015. In that time frame of two months, we identified 23 magnetic holes. The cometary activity was intermediate and increasing because Rosetta was on the inbound leg toward the Sun. While in April solar wind protons were still observed by Rosetta near the comet, in May these protons were already mostly replaced by heavy cometary ions. Magnetic holes have frequently been observed in the solar wind. We find, for the first time, that magnetic holes exist in the cometary environment even when solar wind protons are almost absent. Some of the properties of the magnetic holes are comparable to those of solar wind holes; they are associated with density enhancements, sometimes associated with co-located current sheets and fast solar wind streams, and are of similar scales. However, particularly in May, the magnetic holes near the comet appear to be more processed, featuring shifted density enhancements and, sometimes, bipolar signatures in magnetic field strength rather than simple depressions. The magnetic holes are of global size with respect to the coma. However, at the comet, they are compressed owing to magnetic field pile-up and draping so that they change in shape. There, the magnetic holes become of comparable size to heavy cometary ion gyroradii, potentially enabling kinetic interactions.
Statistical analysis of UV spectra of a quiescent prominence observed by IRIS
Authors: Jejčič S. , Schwartz P. , Heinzel P. , Zapiór M. , Gunár S.
Published: July 2018

Context. The paper analyzes the structure and dynamics of a quiescent prominence that occurred on October 22, 2013 and was observed by several instruments including the Interface Region Imaging Spectrograph (IRIS).

Aims. We aim to determine the physical characteristics of the observed prominence using Mg II k and h (2796 and 2803 Å), C II (1334 and 1336 Å), and Si IV (1394 Å) lines observed by IRIS. In addition we study the dynamical behavior of the prominence.

Methods. We employed the one-dimensional non-LTE (departures from the local thermodynamic equilibrium – LTE) modeling of Mg II lines assuming static isothermal-isobaric slabs. We selected a large grid of models with realistic input parameters expected for quiescent prominences (temperature, gas pressure, effective thickness, microturbulent velocity, height above the solar surface) and computed synthetic Mg II lines. The method of Scargle periodograms was used to detect possible prominence oscillations.

Results. We analyzed 2160 points of the observed prominence in five different sections along the slit averaged over ten pixels due to low signal to noise ratio in the C II and Si IV lines. We computed the integrated intensity for all studied lines, while the central intensity and reversal ratio was determined only for both Mg II and C II 1334 lines. We plotted several correlations: time evolution of the integrated intensities and central intensities, scatter plots between all combinations of line integrated intensities, and reversal ratio as a function of integrated intensity. We also compared Mg II observations with the models. Results show that more than two-thirds of Mg II profiles and about one-half of C II 1334 profiles are reversed. Profiles of Si IV are generally unreversed. The Mg II and C II lines are optically thick, while the Si IV line is optically thin.

Conclusions. The studied prominence shows no global oscillations in the Mg II and C II lines. Therefore, the observed time variations are caused by random motions of fine structures with velocities up to 10 km s−1. The observed average ratio of Mg II k to Mg II h line intensities can be used to determine the prominence’s characteristic temperature. Certain disagreements between observed and synthetic line intensities of Mg II lines point to the necessity of using more complex two-dimensional multi-thread modeling in the future.
Single stage en bloc resection of a recurrent metastatic osteosarcoma of the pediatric lumbar spine through multiple exposures – a novel approach
Authors: Gupta Saurabh , Stinson Zachary S. , Marco Rex A. , Dormans John P.
Published: July 2018

To obtain a wide resection and safe margins in recurrent spine osteosarcoma, the surgical approach can include – posterior only, combined posterior and anterior, and combined posterior and anterior with a return to posterior in multiple stages. In our case, we used a novel approach of multiple extensile exposures circumferentially in a single stage with a single surgical prep. We present the case of a 9-year-old female with a history of metastatic osteosarcoma, who previously underwent an attempted en bloc resection with an L3 corpectomy and left below knee amputation. At 1 year follow-up, she developed a recurrent solitary spine lesion at the previous surgical resection site. An additional attempt at complete surgical resection was performed with a complex en bloc L2, L3, L4 corpectomy with removal of deep spinal implants and anterior and posterior spinal fusion with instrumentation and revision decompressive laminectomy. The patient had a good functional outcome without neurological deficits, except those resulting from resection of involved lumbar nerve roots. At last follow-up of 5 months, there was no local recurrence or distant metastasis. This approach for revision resection of recurrent spinal osteosarcoma can be performed successfully with clean margins in a safe manner.
Merkelzellkarzinome der Kopf-Hals-Region
Authors: Steinbichler Teresa B. , Hauser Undine , Schartinger Volker H. , Dudás József , Riechelmann Herbert
Published: July 2018

Merkelzellkarzinome (MCC) sind hochmaligne neuroendokrine Tumore der Haut. Auch wenn sie sehr selten sind, hat sich ihre Inzidenz in den letzten 20 Jahren
verdoppelt. Ihre Mortalität ist höher als die des malignen Melanoms. Risikofaktor ist neben UV-Exposition eine iatrogene oder anderweitige Immunsuppression. MCC
betreffen vor allem Menschen jenseits des 70. Lebensjahrs und treten in der Hälfte der Fälle in der Kopf-Hals-Region auf.
Immunonkologie – ein Überblick
Authors: Kobold Sebastian , Krackhardt Angela , Schlösser Hans , Wolf Dominik
Published: July 2018

Pioneering work has unraveled the role of the immune system in the development and control of cancer. This knowledge has set the basis for the successful implementation of immunotherapy into the standard of care for a large number of cancer types. Based on response rates and prolongation of overall survival, immunotherapeutic approaches have been approved in a growing number of tumor diseases. Activation or therapeutic utilization of T cells represent the basis of these concepts. Checkpoint inhibitory antibodies inhibiting CTLA-4, PD1 and PD-L1 receptors and ligands induce long-term clinical tumor control in a significant number of cancer patients including metastatic melanoma and non-small cell lung cancer. As an alternative concept of T cell activation, the dual CD19 – CD3 targeting bispecific antibody blinatumomab has been approved for refractory acute lymphatic B-cell leukemia (ALL). Moreover, T cells genetically engineered with an anti-CD19-chimeric antigen receptor have also been approved for ALL and malignant B-cell lymphoma by the food and drug administration (FDA). In all of these immunotherapies, severe side effects may occur and require a well-trained team of physicians. Moreover, the growing number of clinically investigated and validated novel compounds as well as cellular therapeutics accentuate the complexity and challenge of this treatment modality. This review highlights the most prominent recent clinical developments in the field of immuno-oncology.
Kieferorthopädischer Lückenschluss als Therapieoption bei fehlenden oberen seitlichen Schneidezähnen – ein Fallbericht
Authors: Jäger Karin
Published: July 2018

Der vorliegende Fallbericht zeigt die Therapie und das Behandlungsergebnis eines jugendlichen Patienten mit Agenesie der oberen seitlichen Schneidezähne. Der 14 Jahre alte, männliche Patient wies eine dentale Klasse-I-Malokklusion mit milder skelettaler Klasse III und vertikalem Wachstumsmuster auf. Der Behandlungsplan sah einen Lückenschluss im Oberkiefer und den Ersatz der seitlichen Schneidezähne durch die Eckzähne vor. Im Anschluss an die kieferorthopädische Behandlung war eine prothetische Umgestaltung der oberen Eckzähne und der ersten oberen Prämolaren geplant.
Brodie-Bite-Syndrom – Fallbericht inklusive Nachuntersuchung 23 Jahre nach Behandlung
Authors: Cudovic Borko , Savio Thorsten
Published: July 2018

Die Behandlung dieses Patienten mit Angle Klasse II/2, totaler Nonokklusion und Extraktion aller zweiten Molaren 7±7 erfolgte mittels Edgewise-Apparatur mit 0.022×0.028 inch Slot. Von größter Bedeutung war die Kontrolle der vertikalen Dimension. Zudem waren Funktion, Stabilität, faziale Balance und Harmonie ein ultimatives Ziel der Behandlung.
Rapid and Simultaneous Determination of Three Active Components in Raw and Processed Root Samples of Scutellaria baicalensis by Near-infrared Spectroscopy
Authors: Jintao Xue , Quanwei Yang , Chunyan Li , Yun Jing , Shuangxi Wang , Mingxiang Zhang , Peng Li
Published: July 2018

Motivated by the wide use of Scutellariae Radix (SR) in the food and pharmaceutical industries, a rapid and non-destructive near-infrared spectroscopy (NIRS) method was developed for the simultaneous analysis of three main active components in raw SR and SR processed by stir-frying with wine. From seven geographical areas, 58 samples were collected. The reference contents for the SR components baicalin, baicalein, and wogonin were determined by high-performance liquid chromatography. Two multivariate analysis methods, partial least-squares (PLS) regression as a linear regression method and artificial neural networks (ANN) as a nonlinear regression method, were applied to the NIR data, and their results were compared. In the PLS model, different model parameters (i.e., 11 spectral pre-treatment methods), spectral region, and latent variables were investigated to optimize the calibration model; additionally, the ANN model was applied with five different spectral pre-treatment methods and six algorithms. For the optimal model parameters, the correlation coefficients of the calibration set for baicalin, baicalein, and wogonin were 0.9979, 0.9786, and 0.9773, respectively; the correlation coefficients of the prediction set were 0.9756, 0.9843, and 0.9592, respectively; the root mean square error of validation values were 0.215, 0.321, and 0.174, respectively. The optimal NIR models were then employed to analyze the effects of processing and geographical regions on analyte contents. The established NIR methods were robust, accurate, and reproducible. NIRS may be a promising approach for the routine screening and quality control of traditional Chinese medicines.
A Unique Skeletal Rearrangement of a Bicyclo[3.3.1]nonanetrione to a Tetrahydroquinolin-2(1H)-one System
Authors: Wu Jing , Liang Yong , Li Jian-Xin , Shen Qi , Liu Fang , Zhang Yu-Chao , Wang Jie , Zhang Lei , Wang Yue , Xu Hong-Xi , Shao Zhuzhou , Cao Yang
Published: July 2018

The unexpected formation of a 4-hydroxytetrahydroquinolin-2(1H)-one from a bicyclo[3.3.1]nonanetrione system and an amino alcohol in the presence of TsOH is reported. The mechanism of this transformation was studied by DFT calculations. The reaction provides an entry to the synthesis of highly functionalized 4-hydroxytetrahydroquinolin-2(1H)-ones.
Synthesis of the First Representatives of Thieno[3,2-c][1,7]naphthyridine Derivatives Based on 3-Amino-6-methyl-4-(2-thienyl) pyridin-2(1H)-one
Authors: Kulakov Ivan , Matsukevich Mariya , Levin Maxim , Palamarchuk Irina , Seilkhanov Tulegen , Fisyuk Alexander
Published: July 2018

A one-pot method for obtaining novel thieno[3,2-c][1,7]naphthyridine derivatives based on the reaction of 3-amino-4-(thien-2-yl)pyridin-2(1H)-one with aromatic aldehydes in 80% ­phosphoric acid at 130 °C has been developed. The formation of the thieno[3,2-c][1,7]naphthyridine ring was due to the intermediate generation of the corresponding azomethine, which underwent intra­molecular cyclization with electrophilic attack of the β-carbon atom of the thiophene core under Pictet–Spengler conditions. The isolated 5,7-dihydrothieno[3,2-c][1,7]naphthyridin-4(3H)-ones underwent oxidative aromatization in air to give thieno[3,2-c][1,7]naphthyridin-6(7H)-ones. A two-step synthesis of thieno[3,2-c][1,7]naphthyridines involving the isolation of the intermediate imine did not lead to a significant increase in the product yield.
Intraoperative Seizures in Awake Craniotomy for Perirolandic Glioma Resections That Undergo Cortical Mapping
Authors: Eseonu Chikezie , Rincon-Torroella Jordina , Lee Young , ReFaey Karim , Tripathi Punita , Quinones-Hinojosa Alfredo
Published: July 2018

Background Perirolandic motor area gliomas present invasive eloquent region tumors within the precentral gyrus that are difficult to resect without causing neurologic deficits.

Study Aims This study evaluates the role of awake craniotomy and motor mapping on neurologic outcome and extent of resection (EOR) of tumor in the perirolandic motor region. It also analyzes preoperative risk factors for intraoperative seizures.

Methods We evaluated 57 patients who underwent an awake craniotomy for a perirolandic motor area eloquent region glioma. Patients who had positive mapping (PM) or intraoperative identification of motor regions in the cortex using direct cortical stimulation were compared with patients with no positive motor mapping following direct cortical stimulation and negative mapping (NM). Preoperative risks, intraoperative seizures, perioperative outcomes, tumor characteristics, and EOR were also compared. A logistic regression model was used to evaluate the predictors for intraoperative seizures in this patient cohort.

Results Overall, 33 patients were in the PM cohort; 24 were in the NM cohort. Our study showed an 8.8% incidence of intraoperative seizures during cortical and subcortical mapping for awake craniotomies in the perirolandic motor area, none of which aborted the case. PM patients had significantly more intraoperative and postoperative seizures (15.5% and 30.3%, respectively) compared with the NM patients (0% and 8.3%, respectively; p = 0.046 and 0.044). New transient postoperative motor deficits were found more often in the PM group (51.5%) versus the NM group (12.5%; p = 0.002). A univariate logistic regression showed that PM (odds ratio [OR]: 1.16; 95% confidence interval [CI], 1.01–1.34; p = 0.035) and preoperative tumor volume (OR: 0.998; 95% CI, 0.996–0.999; p = 0.049) were significant predictors for intraoperative seizures in patients with perirolandic gliomas.

Conclusion Awake craniotomies in the perirolandic motor region can be safely performed with a similar incidence of intraoperative seizures as reported for the language cortex. PM in this region may increase the likelihood of perioperative seizures or motor deficits compared with NM. Craniotomies that minimize cortical exposure for perirolandic gliomas that may not localize motor regions can still allow for extensive tumor resection with a good postoperative outcome.
Diagnosis of Lumbar Spinal Stenosis with Functional Myelography
Authors: Frantz Sandra , Dezena Roberto , Pereira Carlos , Tatagiba Marcos , Morgalla Matthias
Published: July 2018

Background and Study Aims The diagnosis of a lumbar spinal stenosis demands advanced diagnostic radiologic techniques. In recent decades magnetic resonance imaging (MRI) has replaced myelography, now considered an old-fashioned technique. It was our hypothesis that functional myelography still plays an important role in selected cases. We investigated how our surgical strategy was influenced by the results of MRI, functional myelography, and postmyelography computed tomography (CT) in patients with a lumbar spinal stenosis.

Methods The sagittal diameters of the lumbar spinal canal were measured from L1 to S1 on patients with lumbar spinal stenosis. MRI, functional myelography, and postmyelography CT were compared in each of the patients. Sensitivity and specificity were calculated in each method. We examined how the surgical strategy was influenced by the results of these different methods.

Results Fifty consecutive patients (21 women and 29 men; mean age: 70 years, [range: 49–86 years]) fulfilled the inclusion criteria. Functional myelography revealed a sensitivity of 0.99, a specificity of 0.79, and a positive predictive value of 0.45. The MRI exhibited a sensitivity of 0.93, a specificity of 0.74, and a positive predictive value of 0.39. Postmyelography CT showed a sensitivity of 0.96, a specificity of 0.75, and a positive predictive value of 0.41. A functional myelography revealed more information than the MRI and resulted in a change in the surgical strategy in 11 of 50 patients (22%) in comparison with the sole results of MRI or a postmyelography CT.

Conclusions In selected cases with multilevel lumbar spinal stenosis, functional myelography revealed the highest precision in reaching a correct diagnosis. It resulted in a change in the surgical approach in every fifth patient in comparison with the MRI and proved most helpful, especially in elderly patients.
Spontaneous Intraventricular Hemorrhage Caused by Sphenoid Meningioma
Authors: Laaguili Jawad , Gazzaz Miloudi , Mostarchid Brahim , Mandour Cherkaoui
Published: July 2018

Introduction Benign sphenoid meningioma presenting with an acute intraventricular hemorrhage is extremely uncommon.

Case report We report a case of a 61-year-old man who was admitted after sudden onset of a severe headache. Brain computed tomography revealed a sphenoid meningioma with acute intraventricular hemorrhage.

Discussion Intraventricular hemorrhage caused by Meningiomas is infrequent. Several hypotheses have been proposed to explain the cause of hemorrhage.

Conclusion The benignity of meningiomas is relative because these tumors can cause exceptional complications such as bleeding.
Marathon Run III: Effects on Coagulation, Fibrinolysis, Platelet Aggregation and Serum Cortisol Levels
Authors: Mandalaki T , Dessypris A , Louizou C , Panayotopoulou C , Dimitriadou C
Published: July 2018

Blood coagulation, fibrinolysis, platelet count-aggregation and Cortisol were assessed in 35 Finnish amateur runners aged 27 to 56 years (mean 40) and three aged 65, 67, and 82 who had run a non-competitive Marathon in 1975, 1976 and 1977 over the classical itinerary. After the run, in all 3 years, APTT showed shortening (p < 0.001); prothrombin time and plasma fibrinogen were not significantly altered; euglobulin lysis time was shorter (p < 0.001) and FDP increased (p < 0.001); PSPT became positive in all subjects, whereas the ethanol gelation test remained negative; no cryofibrinogen was detected. Platelet count and aggregation showed increase (p < 0.001) in 1975 (extreme heat, 25° C) but remained unaltered in 1976, 1977 (15–18° C). Cortisol levels were always significantly increased – more markedly in 1975. Women’s responses were similar to those of men. A possible correlation between physical fitness and the responses of haemostatic balance is suggested and the influence of weather is discussed.
The Platelet of the Newborn Infant – Adenine Nucleotide Metabolism and Release
Authors: Whaun J M , Lievaart P ,   with the technical assistance of
Published: July 2018

Blood from normal full term infants, mothers and normal adults was collected in citrate. Citrated platelet-rich plasma was prelabelled with 3H-adenine and reacted with release inducers, collagen and adrenaline. Adenine nucleotide metabolism, total adenine nucleotide levels and changes in sizes of these pools were determined in platelets from these three groups of subjects.

At rest, the platelet of the newborn infant, compared to that of the mother and normal adult, possessed similar amounts of adenosine triphosphate (ATP), 4.6 ± 0.2 (SD), 5.0 ± 1.1, 4.9 ± 0.6 µmoles ATP/1011 platelets respectively, and adenosine diphosphate (ADP), 2.4 ± 0.7, 2.8 ± 0.6, 3.0 ± 0.3 umoles ADP/1011 platelets respectively. However the marked elevation of specific radioactivity of ADP and ATP in these resting platelets indicated the platelet of the neonate has decreased adenine nucleotide stores.

In addition to these decreased stores of adenine nucleotides, infant platelets showed significantly impaired release of ADP and ATP on exposure to collagen. The release of ADP in infants, mothers, and other adults was 0.9 ± 0.5 (SD), 1.5 ± 0.5, 1.5 ± 0.1 umoles/1011 platelets respectively; that of ATP was 0.6 ± 0.3, 1.0 ± 0.1,1.3 ± 0.2 µmoles/1011 platelets respectively. With collagen-induced release, platelets of newborn infants compared to those of other subjects showed only slight increased specific radioactivities of adenine nucleotides over basal levels. The content of metabolic hypoxanthine, a breakdown product of adenine nucleotides, increased in both platelets and plasma in all subjects studied.

In contrast, with adrenaline as release inducer, the platelets of the newborn infant showed no adenine nucleotide release, no change in total ATP and level of radioactive hypoxanthine, and minimal change in total ADP. The reason for this decreased adrenaline reactivity of infant platelets compared to reactivity of adult platelets is unknown.

Infant platelets may have different membranes, with resulting differences in regulation of cellular processes, or alternatively, may be refractory to catecholamines because of elevated levels of circulating catecholamines in the newborn period.
A Sex Difference in the Bleeding Time
Authors: Bain Barbara , Forster T
Published: July 2018

The bleeding time by a modified Ivy technique was estimated on 128 normal volunteers, 59 men and 69 women. The bleeding time was found to be significantly longer in women than men (p<.001). This was not attributable to a difference in platelet count nor to any detectable difference in platelet aggregability. It is likely that a difference in blood vessels or supporting tissues is responsible for the observed difference.
Heterogeneity of the "Classical" Antithrombin III Deficiency
Authors: Sas Géza , Petö Iván , Bánhegyi Denes , Blaskó György , Domján Gyula
Published: July 2018

We investigated two thrombophilia families with the "classical" type of antithrombin III deficiency, i.e., with a low antithrombin III level measured both by immunochemical and functional methods.

We obtained different antithrombin III patterns in the plasma of the affected members of the two families with the modified two dimensional immunoelectrophoresis method (heparin in agarose). In one family, the electrophoretic mobility of the antithrombin III is identical with that of normal antithrombin III. In the other, the antithrombin III displayed a decreased electrophoretic mobility in the heparinized agarose gel. The relatively low affinity of this antithrombin III to heparin could be directly proved by the heparin-agarose affinity chromatography, too. These two different antithrombin III patterns were observed by other investigators at different families as well.

On the basis of our simultaneous observations of these two families we propose a classification of the inherited congenital antithrombin III deficiencies.
AB0 Blood Groups and Coronary Heart Disease (CHD)
Authors: Erikssen Jan , Thaulow Erik , Stormorken Helge , Brendemoen Ole , Hellem Arvid
Published: July 2018

The view based on epidemiological and laboratory data that blood group A subjects (=A) have clinically significant higher thrombotic potential than blood group 0 subjects (= O), is supported by the present finding of a significantly higher platelet retention in A than 0.

The completely normal ABO distribution found among 71 cases of proven latent CHD, and the disproportionate excess of 0 vs. A in a consecutive series of 191 coronary artery bypass candidates apparently conflict with epidemiological data indicating a higher risk of achieving CHD in A than 0. The conflict may be solved by suggesting

a) that the »thrombotic proneness« in A compared with 0 causes a poorer prognosis in CHD among the former, leaving a disproportionate excess of 0 among longterm CHD survivors, and b) that AB0-related factors have had an insignificant, independent impact on the evolution of preclinical coronary artery disease in our 71 men with latent CHD.
Studies of Aggregation and Thromboxane B2 Synthesis in Avian Thrombocytes: Comparison between Atherosclerosis-Susceptible and Resistant Breeds
Authors: Subbiah M T R , Yunker R , Gallon L
Published: July 2018

Comparison of thrombocyte aggregation in response to arachidonic acid and synthesis of prostaglandins from C14-arachidonic acid by thrombocytes was made between atherosclerosis-susceptible White Carneau and atherosclerosis-resistant Show Racer Pigeons. Contrary to the expectations, thrombocytes from atherosclerosis-susceptible pigeons were a) less sensitive to arachidonic acid induced aggregation and b) synthesized less thromboxane B2 (stable product of proaggregatory thromboxane A2) from C14-arachidonic acid. Analysis of fatty acid composition of thrombocyte phospholipids indicated a higher concentration of arachidonic acid in White Carneau Pigeons. These results suggest that thrombocytes from atherosclerosis-susceptible pigeons are altered early and as a defense mechanism display less responsiveness towards aggregation.
Lipid Activation of Coagulation Factor III Apoprotein (Tissue Factor) - Reconstitution of the Protein-Membrane Complex
Authors: Carson Steven D , Konigsberg William H
Published: July 2018

Coagulation factor III (tissue factor) is required for significant activation of factor X in the presence of factor VIIa. Factor III is a membrane protein which requires bound phospholipids for activity, and removal of lipids during purification of factor III abolishes its activity. The activity can be recovered if lipids are added to the apoprotein under conditions known to favor reconstitution of membrane proteins into phospholipid bilayers. Indeed, incorporation of factor III into vesicular phospholipids is the essential event for recovery of factor III function, and is promoted by cadmium chloride.

Using factor III from human placenta, we have studied the interactions of cadmium, phospholipids, and apoprotein which result in successful protein membrane reconstitution. The effects of phospholipids and cadmium can be independently optimized. Cadmium promotes reconstitution of factor III either with preformed phospholipid vesicles or as the vesicles form during slow dialysis. The incorporation of apoprotein into preformed vesicles is completed within four minutes. These studies demonstrate successful activation of factor III apoprotein by incorporation into phospholipid vesicles and optimization of this activation using cadmium chloride.
A Survey of the Effectiveness of Prothrombin Complex Concentrates in Controlling Hemorrhage in Patients with Hemophilia and Anti-Factor VIII Antibodies
Authors: Blatt Philip M , Ménaché Doris , Roberts Harold R
Published: July 2018

The treatment of patients with hemophilia A and anti-Factor VIII antibodies is difficult. Between July 1977 and June 1978, a survey was carried out by an ad hoc working party of the subcommittee on Factor IX concentrates of the International Committee on Thrombosis and Hemostasis to assess the effectiveness of Prothrombin Complex Concentrates in controlling hemorrhage in these patients. The results are presented in this paper and, although subjective, support the view that these concentrates are not as effective in patients with inhibitors as Factor VIII concentrates are in patients without inhibitors.
Malondialdehyde Formation in Rat Platelet-Rich Plasma
Authors: Rajtar G , Livio M , Merino J , Gaetano G de
Published: July 2018

Malondialdehyde (MDA) is a stable product of arachidonic acid metabolism, catalyzed by the enzyme cyclo-oxygenase. The experimental conditions for measuring the kinetics of MDA formation in rat citrated platelet-rich plasma were defined. Platelets were stimulated with either arachidonic acid, the substrate of MDA, or thrombin, an enzyme which induces release of free arachidonic acid from platelet membrane phospholipids. MDA formation was almost linear for a limited period of time (between 0 and 2 min with arachidonic acid and between 1 and 3 min with thrombin) and was concentration-dependent with saturation kinetics.

The hyperbolic curves obtained could be recast in linear plots (according to Woolf transformation S/V versus S) when arachidonic acid was used. With thrombin, in contrast, the highest concentration at which no MDA production could be detected (3 NIH u/ml) had to be subtracted from each concentration of the enzyme used to obtain Woolf plots. The apparent Km value of arachidonic acid was 0.49 ± 0.09 mM and Vmax was 1.44 ± 0.06 nmoles MDA/1.4 × 109 platelets/min. The corresponding values in experiments with thrombin were 6.5 ± 1.5 NIH u/ml and 0.233 ± 0.012 nmoles MDA/1.4 × 109 platelets/min.
Kinetics and in Vivo Redistribution of 111Indium-Labelled Human Platelets after Intravenous Protamine Sulphate
Authors: Heyns A duP , Lötter M G , Badenhorst P N , Kotze H , Killian F C , Herbst C , Reenen O R van , Minnaar P C
Published: July 2018

The pathogenesis of thrombocytopenia induced by intravenous protamine sulphate was studied in six patients who underwent cardiopulmonary bypass surgery, and in three normal volunteers. Autologous platelets were labelled with 111Indium-oxine. Platelet lifespan was determined. In vivo 111In-platelet localization, organ redistribution and sites of destruction were quantitated with a scintillation camera and a computer-assisted imaging system. Protamine induced a transient thrombocytopenia, maximal 5-10 min after injection, and 30-40 min in duration. The thrombocytopenia was accompanied by a transient accumulation of platelets in the liver. The splenic platelet pool remained unaltered and no platelets accumulated in the lungs. Platelet survival, measured in two volunteers, was slightly longer than normal and fitted a linear function best. There was a severe transient neutropenia during the period of thrombocytopenia. We conclude that protamineinduced thrombocytopenia is caused by hepatic accumulation of "activated" platelets or platelet aggregates, the process is reversible, and in the two normal volunteers studied, platelet survival was not affected.
Increased Fibrinopeptide A after Prothrombin Complex Concentrates
Authors: Viganó S , Cattaneo M , Gervasoni W , Mannucci P M
Published: July 2018

Prothrombin complex concentrates (PCC) are known to carry a risk of thromboembolism. We have chosen to reassess the problem of detecting in vivo signs of activation of the clotting mechanism by assaying fibrinopeptide A (FpA) after PCC administration in hemophilic patients during bleeding episodes. FpA was significantly increased above baseline levels 15 to 60 min after the infusion of 19 doses of 5 different types of commercial PCC in 14 hemophilia B patients treated for bleeding episodes or dental extractions. A more marked increase followed 16 infusions of the activated PCC FEIBA and Auto IX in 4 hemophilia A patients with F. VIII inhibitors. There was no significant FpA change after F. VIII concentrates administred to a control group of 7 patients with hemophilia A. These findings suggest that circulation of thrombin occurs frequently after PCC administration, even though clinical manifestations of thromboembolism appear to be relatively rare.
Platelet Malondialdehyde in Cardiovascular Disease: Effect of Prosthetic Heart Valves and Cardioactive Drugs on Production
Authors: Sullivan Jay M , Taylor J Charles , Samaha Joseph K , Lamar Patti , Patrick Darrell R ,   With the technical assistance of
Published: July 2018

Malondialdehyde (MDA), a product of platelet lipid peroxidation, was measured in human platelet-rich plasma. Levels of 3.19 n moles/109 platelets ± 0.40 S.E. in 11 patients with prosthetic heart valves were elevated (p<0.25) compared to 17 normal subjects (2.09 ± 0.13 n moles/109 platelets). Reduced production (1.44 ± 0.28 n moles/109 platelets, p<0.5) was found in 10 patients with unstable angina. Normal levels were found in patients with mitral stenosis, cardiomyopathy or hypertension. Usual serum levels of drugs used in cardiac treatment reduced MDA levels as follows: acetaminophen, 47% (p<.01); aspirin, 58% (p<.05); furosemide, 32.6% (p<.005), and sulfinpyrazone, 41% (p<.05). Digoxin, dipyridamole, heparin, hydrochlorothiazide, lidocaine, nitroglycerin, procainamide, propranolol, quinidine, or warfarin had no significant effect at therapeutic concentrations. None of the drugs explained the enhanced production in patients with prosthetic valves while analgesic therapy could explain the decreased levels in other cardiacs. The half-time of platelet survival, measured by suppression of malondialdehyde production, was 3.2 ± 0.24 days in 9 normal subjects but could not be measured reliably in most patients because of multiple drug therapies. We conclude that the blood platelets of patients with prosthetic heart valves differ from those of normal subjects in their capacity to release malondialdehyde after stimulation with n-ethylmaleimide. Additionally, we find that inhibition of malondialdehyde production by several pharmacologic agents limits the usefulness of this method for the measurement of platelet survival in cardiac patients.
Reactivity of a Hereditary Abnormal Antithrombin III Fraction in the Inhibition of Thrombin and Factor Xa
Authors: Tran T H , Bondeli C , Marbet G A , Duckert F
Published: July 2018

Two different AT-III fractions were purified from the plasma of a patient with recurrent superficial thrombophlebitis. The abnormal AT-III fraction (A-AT) was compared to the normal AT-III fraction (N-AT) in the inhibition of thrombin and factor Xa. Without heparin, both inactivate proteases in a similar manner and at the same rate. However, at low heparin concentration the thrombin inhibition proceeds more slowly with A-AT than with N-AT. At high heparin concentration the difference between A-AT and N-AT becomes very small. The inhibition of factor Xa follows a similar pattern. It is suggested that the heparin binding site of A-AT differs from that of N-AT resulting in a decreased heparin cofactor activity.
Platelet Function in Acute Myocardial Infarction Patients Compared with Controls
Authors: O'Brien John R , Etherington Michael D , Shuttleworth Robert D , Calwell William H
Published: July 2018

A considerable number of tests were carried out comparing 33 patients with acute myocardial infarction (MI), 13 patients with chest pain (CP) and 47 controls. There was some evidence that the plasma platelet factor 4 (PF4) was different in the three groups. The heparin neutralizing activity (HNA) of platelet poor plasma was increased in the MI patients relative to the controls and the intra-platelet HNA was decreased. Malondialdehyde (MDA) formation by platelets maximally stimulated by thrombin was decreased in MI whilst the plasma 5-hydroxyindoles (5HIs) in MI was greatly increased. The acute phase proteins α1 acid glycoprotein and fibrinogen were significantly raised in MI. The results in the CP group were intermediate between the MI and the controls. These findings define more precisely the changes in acute MI and are relevant to the concept of exhausted platelets.
Kinetics and Fate of 111Indium-Oxine Labelled Blood Platelets in Asplenic Subjects
Authors: Heyns Anthon duP , Lötter Matthys G , Badenhorst Philip N , Reenen Otto van , Pieters Henry , Minnaar Phil C
Published: July 2018

The survival, tissue distribution and fate of 111Indium-oxine labelled autologous platelets was studied in four asplenic subjects with serial blood sampling, scintillation camera and computer-assisted imaging. Mean 111In-platelet recovery in the circulation was 89 ± 13% (± 1 SD). Platelet survival curves fitted a linear function best and was 238 ± 41 h. The shape of the survival curves of normal and asplenic subjects differed: in the asplenic subjects the curve was linear whereas that of normal subjects was significantly more curvilinear if analyzed by least squares computer fitting to a gamma function. Early hepatic 111In-activity was significant and transient and ascribed to the "collection injury". As labelled platelets disappeared from the circulation, 111Inactivity in the liver increased progressively and linearly to reach 42.5 ± 14.1 % of whole body activity at 240 h. Radioactivity also accumulated in the bone marrow, but could not be demonstrated in the vasculature of the lower limbs. These results would indicate that in asplenic subjects the major sites of destruction of senescent platelets are the liver and bone marrow.
Soluble Fibrin Complexes and Fibrinogen Heterogeneity in Diabetes mellitus
Authors: Tsianos E B , Stathakis N E
Published: July 2018

The presence of soluble fibrin complexes (SFC) measured by gel filtration of plasma on 4% agarose columns, fibrinogen heterogeneity on 3.5% SDS-polyacrylamide gels and the concentrations of several plasma proteins were evaluated in 39 patients with diabetes mellitus (DM) and 19 matched control subjects. A small but significant increase of SFC was found in DM (p<0.01). On individual basis 51.2% of the patients had increased SFC (>M + 2 SD of the controls). Polyacrylamide gel electrophoresis of the SFC showed no evidence of cross-linking or proteolysis. Plasma clots formed in the presence of EDTA and trasylol were analysed in SDS-polyacrylamide gels in a normal and two lower molecular weight fibrin bands (band I, II, III). The percentage of band I fibrinogen was in diabetics (65.3 ± 4.7%) lower than that of the controls (71.8 ± 4.5%) (p < 0.01). Fibrinogen levels, antithrombin III, α1-antitrypsin, α2-macroglobulin and plasminogen were significantly increased in DM. We suggest that in DM there is an enhancement of intravascular fibrin formation and accelerated fibrinogen degradation to lower molecular weight forms.
Predictive Value of Decreasing Antithrombin III in Deep-Vein Thrombosis
Authors: Andreasen Thorkild Lund
Published: July 2018

Antithrombin III (At-III) was measured at the time of admission and two days later in 131 patients laid up in a coronary care unit. The patients were examined for deep-vein thrombosis (DVT) clinically and by means of 125I-fibrinogen scanning. 19 patients developed DVT. In 11 subjects with and 25 without DVT At-III decreased more than 10%. And in 7 with and 17 without DVT At-III decreased more than 15%. One person with DVT had subnormal At-III. By using decrease of At-III or subnormal initial At-III to predict DVT the following predictive value (PV) were found. Decrease ≤ 10%, PV pos.= 0.32 and PV neg. = 0.93. Decrease ≤ 15%, PV pos. = 0.32 and PV neg. = 0.90. The positive predictive values obtained were too low to let decreasing At-III give occasion for prophylactic anticoagulant treatment.
Evaluation of the Effect of Betapropiolactone/Ultraviolet Irradiation (BPL/UV) Treatment of Source Plasma on Hepatitis Transmission by Factor IX Complex in Chimpanzees
Authors: Prince Alfred M , Stephan W , Brotman B , Ende M C van den
Published: July 2018

To evaluate the safety of a β-Propiolactone/Ultraviolet (BPL/UV), irradiated Factor IX complex preparation we inoculated 8 chimpanzees with 25 units Factor IX/Kilo from a pool of 5 production lots which had been treated in this manner. These lots were derived from approximately 1,000 donors. Animals were followed with weekly tests for hepatitis B serologic markers and transaminases, and biweekly liver biopsies, for 6 months. No evidence of transmission of hepatitis B, or non-A, non-B viruses was observed.

To further evaluate the BPL/UV procedure a plasma pool was intentionally contaminated with hepatitis B virus and one half of the pool treated with BPL/UV. Factor IX complex was isolated from the treated and untreated pools and each was inoculated into 4 chimpanzees. The Factor IX derived from untreated plasma infected all four animals with an average incubation period of 10.5 weeks, whereas that prepared from BPL/UV treated plasma infected only one of four animals with an incubation period of 21 weeks. These results were interpreted as suggesting that BPL/UV can inactivate approximately 99.9% of hepatitis B virus infectivity.
Effects of Halothane on Platelet Function
Authors: Dalsgaard-Nielsen J , Gormsen J
Published: July 2018

Human platelets in platelet rich plasma (PRP) incubated at 37° C with 0.3–2% halothane for 5–10 min lost the ability to aggregate with ADP, epinephrine and collagen.

At the same time uptake and release of 14C-serotonin was inhibited. When halothane supply was removed, platelet functions rapidly returned to normal. However, after high concentrations of halothane, the inhibition of platelet aggregation was irreversible or only partially reversible.

The results suggest that halothane anaesthesia produces a transient impairment of platelet function.