bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimicrobial Drug-Resistant Gram-Negative Saprophytic Bacteria Isolated from Ambient, Near-Shore Sediments of an Urbanized Estuary: Absence of β-Lactamase Drug-Resistance Genes
Authors: Moritz Charles F. , Snyder Robert E. , Riley Lee W. , Immke Devin W. , Greenfield Ben K.
Published: July 2020

We assessed the prevalence of antimicrobial resistance and screened for clinically relevant β-lactamase resistance determinants in Gram-negative bacteria from a large urbanized estuary. In contrast to the broad literature documenting potentially hazardous resistance determinants near wastewater treatment discharge points and other local sources of aquatic pollution, we employed a probabilistic survey design to examine ambient, near-shore sediments. We plated environmental samples from 40 intertidal and shallow subtidal areas around San Francisco Bay (California, USA) on drug-supplemented MacConkey agar, and we tested isolates for antimicrobial resistance and presence of clinically relevant β-lactamase resistance determinants. Of the 74 isolates identified, the most frequently recovered taxa were Vibrio spp. (40%), Shewanella spp. (36%), Pseudomonas spp. (11%), and Aeromonas spp. (4%). Of the 55 isolates tested for antimicrobial resistance, the Vibrio spp. showed the most notable resistance profiles. Most (96%) were resistant to ampicillin, and two isolates showed multidrug-resistant phenotypes: V. alginolyticus (cefotaxime, ampicillin, gentamicin, cefoxitin) and V. fluvialis (cefotaxime, ampicillin, cefoxitin). Targeted testing for class 1 integrons and presence of β-lactam-resistance gene variants TEM, SHV, OXA, CTX-M, and Klebsiella pneumonia carbapenemase (KPC) did not reveal any isolates harboring these resistance determinants. Thus, while drug-resistant, Gram-negative bacteria were recovered from ambient sediments, neither clinically relevant strains nor mobile β-lactam resistance determinants were found. This suggests that Gram-negative bacteria in this well-managed, urbanized estuary are unlikely to constitute a major human exposure hazard at this time.
A Dropout Compensation ILC Method for Formation Tracking of Heterogeneous Multi-Agent Systems with Loss of Multiple Communication Packets
Authors: Wu Yuzhou , Zhang Jialu , Ge Yu , Sheng Zhichao , Fang Yong
Published: July 2020

In this paper, the formation tracking problem for heterogeneous multi-agent systems with loss of multiple communication packets is considered using the iterative learning control (ILC) method. A dropout compensation ILC method is presented to construct effective distributed iterative learning protocols. The convergence conditions are given based on the frequency-domain analysis by using the general Nyquist stability criterion and Greshgorin’s disk theorem. The results show that the multi-agent system with different packet loss rate can achieve formation tracking without reducing the convergence speed. Numerical simulation results show the effectiveness of the proposed dropout compensation ILC method.
Practical Enhancement of User Experience in NVMe SSDs
Authors: Kim Seongmin , Kim Kyusik , Shin Heeyoung , Kim Taeseok
Published: July 2020

When processing I/O requests, the current Linux kernel does not adequately consider the urgency of user-centric tasks closely related to user experience. To solve this critical problem, we developed a practical method in this study to enhance user experience in a computing environment wherein non-volatile memory express (NVMe) solid-state drives (SSDs) serve as storage devices. In our proposed scheme, I/O requests that originate from the user-centric tasks were preferentially served across various levels of queues by modifying the multi-queue block I/O layer of the Linux kernel, considering the dispatch method of NVMe SSDs. Our scheme tries to give as fast a path as possible for I/O requests from user-centric tasks among many queues with different levels. Especially, when the SSD is overburdened, it avoids the queues with many pending I/O requests and thus can significantly reduce the I/O latency of user-centric tasks. We implemented our proposed scheme in the Linux kernel and performed practical evaluations on a commercial SSD. The experimental results showed that the proposed scheme achieved significant enhancement in the launch time of five widely used applications by up to ~65%.
The Addition of a Synthetic LPS-Targeting Domain Improves Serum Stability While Maintaining Antimicrobial, Antibiofilm, and Cell Stimulating Properties of an Antimicrobial Peptide
Authors: Maystrenko Anna , Feng Yulong , Akhtar Nadeem , Li Julang
Published: July 2020

Multi-drug resistant (MDR) bacteria and their biofilms are a concern in veterinary and human medicine. Protegrin-1 (PG-1), a potent antimicrobial peptide (AMP) with antimicrobial and immunomodulatory properties, is considered a potential alternative for conventional antibiotics. AMPs are less stable and lose activity in the presence of physiological fluids, such as serum. To improve stability of PG-1, a hybrid peptide, SynPG-1, was designed. The antimicrobial and antibiofilm properties of PG-1 and the PG-1 hybrid against MDR pathogens was analyzed, and activity after incubation with physiological fluids was compared. The effects of these peptides on the IPEC-J2 cell line was also investigated. While PG-1 maintained some activity in 25% serum for 2 h, SynPG-1 was able to retain activity in the same condition for up to 24 h, representing a 12-fold increase in stability. Both peptides had some antibiofilm activity against Escherichia coli and Salmonella typhimurium. While both peptides prevented biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA), neither could destroy MRSA’s pre-formed biofilms. Both peptides maintained activity after incubation with trypsin and porcine gastric fluid, but not intestinal fluid, and stimulated IPEC-J2 cell migration. These findings suggest that SynPG-1 has much better serum stability while maintaining the same antimicrobial potency as PG-1.
The FAAH Inhibitor URB597 Modulates Lipid Mediators in the Brain of Rats with Spontaneous Hypertension
Authors: Biernacki Michał , Baranowska-Kuczko Marta , Niklińska Gabriella N. , Skrzydlewska Elżbieta
Published: July 2020

Hypertension is accompanied by oxidative stress, which can be modified by the functioning of the endocannabinoid system playing a prominent modulatory role in the brain. The present study tested whether chronic administration of the fatty acid amide hydrolase (FAAH) inhibitor [3-(3-carbamoylphenyl) phenyl]N-cyclohexylcarbamate (URB597) to rats with primary hypertension (SHR) can modify redox balance and consequently brain phospholipid metabolism. Experiments were conducted using SHRs and normotensive control Wistar–Kyoto rats treated by intraperitoneal injection with URB597 for 14 days. The biochemical parameters were assayed in the rats’ brains. Inhibition of FAAH activity by URB597 resulted in an increase in anandamide and GPR55 receptor levels, as well as a decrease in CB2 receptor expression. However, there was a simultaneous increase in Nrf2 expression, as well as Cu, Zn-SOD, GSH-Px, glutathione reductase activity, and vitamin E levels in brain tissue of SHR rats. Consequently, URB597 caused a decrease in levels of phospholipid fatty acids and MDA, and an increase in free fatty acids. Given the importance of maintaining redox balance for brain function, the results of this study point to endocannabinoids as a potential therapeutic target for preventing brain metabolic disorders in hypertension.
NLPR3 Inflammasomes and Their Significance for Atherosclerosis
Authors: Poznyak Anastasia V. , Melnichenko Alexandra A. , Wetzker Reinhard , Gerasimova Elena V. , Orekhov Alexander N.
Published: July 2020

Atherosclerosis is a serious disorder, with numerous potential complications such as cardiovascular disease, ischemic stroke, and myocardial infarction. The origin of atherosclerosis is related to chronic inflammation, lipid metabolism alterations, and oxidative stress. Inflammasomes are the cytoplasmic multiprotein complex triggering the activation of inflammatory response. NLRP3 inflammasomes have a specific activation pathway that involves numerous stimuli, including a wide range of PAMPs and DAMPs. Recent studies of atherosclerotic pathology are focused on the mitochondria that appear to be a promising target for therapeutic approach development. Mitochondria are the main source of reactive oxygen species (ROS) associated with oxidative stress. It was previously shown that NLRP3 inflammasome activation results in mitochondrial damage, but the exact mechanisms of this need to be specified. In this review, we focused on the features of NLRP3 inflammasomes and their significance for atherosclerosis, especially concerning mitochondria.
The Role of Alveolar Macrophages in the Improved Protection against Respiratory Syncytial Virus and Pneumococcal Superinfection Induced by the Peptidoglycan of Lactobacillus rhamnosus CRL1505
Authors: Clua Patricia , Tomokiyo Mikado , Raya Tonetti Fernanda , Islam Md. Aminul , García Castillo Valeria , Marcial Guillermo , Salva Susana , Alvarez Susana , Takahashi Hideki , Kurata Shoichiro , Kitazawa Haruki , Villena Julio
Published: July 2020

The nasal priming with nonviable Lactobacillus rhamnosus CRL1505 (NV1505) or its purified peptidoglycan (PG1505) differentially modulates the respiratory innate immune response in infant mice, improving their resistance to primary respiratory syncytial virus (RSV) infection and secondary pneumococcal pneumonia. In association with the protection against RSV-pneumococcal superinfection, it was found that NV1505 or PG1505 significantly enhance the numbers of CD11c+SiglecF+ alveolar macrophages (AMs) producing interferon (IFN)-β. In this work, we aimed to further advance in the characterization of the beneficial effects of NV1505 and PG1505 in the context of a respiratory superinfection by evaluating whether their immunomodulatory properties are dependent on AM functions. Macrophage depletion experiments and a detailed study of their production of cytokines and antiviral factors clearly demonstrated the key role of this immune cell population in the improvement of both the reduction of pathogens loads and the protection against lung tissue damage induced by the immunobiotic CRL1505 strain. Studies at basal conditions during primary RSV or S. pneumoniae infections, as well as during secondary pneumococcal pneumonia, brought the following five notable findings regarding the immunomodulatory effects of NV1505 and PG1505: (a) AMs play a key role in the beneficial modulation of the respiratory innate immune response and protection against RSV infection, (b) AMs are necessary for improved protection against primary and secondary pneumococcal pneumonia, (c) the generation of activated/trained AMs would be essential for the enhanced protection against respiratory pathogens, (d) other immune and nonimmune cell populations in the respiratory tract may contribute to the protection against bacterial and viral infections, and (e) the immunomodulatory properties of NV1505 and PG1505 are strain-specific. These findings significantly improve our knowledge about the immunological mechanisms involved in the modulation of respiratory immunity induced by beneficial microbes.
Toxicoproteomic Profiling of hPXR Transgenic Mice Treated with Rifampicin and Isoniazid
Authors: Brewer Christopher Trent , Kodali Kiran , Wu Jing , Shaw Timothy I. , Peng Junmin , Chen Taosheng
Published: July 2020

Tuberculosis is a global health threat that affects millions of people every year, and treatment-limiting toxicity remains a considerable source of treatment failure. Recent reports have characterized the nature of hPXR-mediated hepatotoxicity and the systemic toxicity of antitubercular drugs. The antitubercular drug isoniazid plays a role in such pathologic states as acute intermittent porphyria, anemia, hepatotoxicity, hypercoagulable states (deep vein thrombosis, pulmonary embolism, or ischemic stroke), pellagra (vitamin B3 deficiency), peripheral neuropathy, and vitamin B6 deficiency. However, the mechanisms by which isoniazid administration leads to these states are unclear. To elucidate the mechanism of rifampicin- and isoniazid-induced liver and systemic injury, we performed tandem mass tag mass spectrometry-based proteomic screening of mPxr−/− and hPXR mice treated with combinations of rifampicin and isoniazid. Proteomic profiling analysis suggested that the hPXR liver proteome is affected by antitubercular therapy to disrupt [Fe–S] cluster assembly machinery, [2Fe–2S] cluster-containing proteins, cytochrome P450 enzymes, heme biosynthesis, homocysteine catabolism, oxidative stress responses, vitamin B3 metabolism, and vitamin B6 metabolism. These novel findings provide insight into the etiology of some of these processes and potential targets for subsequent investigations. Data are available via ProteomeXchange with identifier PXD019505.
The GHSZ Argument: A Gedankenexperiment Requiring More Denken
Authors: Lad Frank
Published: July 2020

I reassess the gedankenexperiment of Greenberger, Horne, Shimony, and Zeilinger after twenty-five years, finding their influential claim to the discovery of an inconsistency inherent in high dimensional formulations of local realism to arise from a fundamental error of logic. They manage this by presuming contradictory premises: that a specific linear combination of four angles involved in their proposed parallel experiments on two pairs of electrons equals both π and 0 at the same time. Ignoring this while presuming the contradictory implications of these two conditions, they introduce the contradiction themselves. The notation they use in their “derivation” is not sufficiently ornate to represent the entanglement in the double electron spin pair problem they design, confounding their error. The situation they propose actually motivates only an understanding of the full array of symmetries involved in their problem. In tandem with the error now recognised in the supposed defiance of Bell’s inequality by quantum probabilities, my reassessment of their work should motivate a reevaluation of the current consensus outlook regarding the principle of local realism and the proposition of hidden variables.
Multiobjective Reactive Power Optimization of Renewable Energy Power Plants Based on Time-and-Space Grouping Method
Authors: Qu Linan , Zhang Shujie , Lin Hsiung-Cheng , Chen Ning , Li Lingling
Published: July 2020

The large-scale renewable energy power plants connected to a weak grid may cause bus voltage fluctuations in the renewable energy power plant and even power grid. Therefore, reactive power compensation is demanded to stabilize the bus voltage and reduce network loss. For this purpose, time-series characteristics of renewable energy power plants are firstly reflected using K-means++ clustering method. The time group behaviors of renewable energy power plants, spatial behaviors of renewable energy generation units, and a time-and-space grouping model of renewable energy power plants are thus established. Then, a mixed-integer optimization method for reactive power compensation in renewable energy power plants is developed based on the second-order cone programming (SOCP). Accordingly, power flow constraints can be simplified to achieve reactive power optimization more efficiently and quickly. Finally, the feasibility and economy for the proposed method are verified by actual renewable energy power plants.
A Comparison of DER Voltage Regulation Technologies Using Real-Time Simulations
Authors: Summers Adam , Johnson Jay , Darbali-Zamora Rachid , Hansen Clifford , Anandan Jithendar , Showalter Chad
Published: July 2020

Grid operators are now considering using distributed energy resources (DERs) to provide distribution voltage regulation rather than installing costly voltage regulation hardware. DER devices include multiple adjustable reactive power control functions, so grid operators have the difficult decision of selecting the best operating mode and settings for the DER. In this work, we develop a novel state estimation-based particle swarm optimization (PSO) for distribution voltage regulation using DER-reactive power setpoints and establish a methodology to validate and compare it against alternative DER control technologies (volt–VAR (VV), extremum seeking control (ESC)) in increasingly higher fidelity environments. Distribution system real-time simulations with virtualized and power hardware-in-the-loop (PHIL)-interfaced DER equipment were run to evaluate the implementations and select the best voltage regulation technique. Each method improved the distribution system voltage profile; VV did not reach the global optimum but the PSO and ESC methods optimized the reactive power contributions of multiple DER devices to approach the optimal solution.
Decreasing Mining Losses for the Room and Pillar Method by Replacing the Inter-Room Pillars by the Construction of Wooden Cribs Filled with Waste Rocks
Authors: Skrzypkowski Krzysztof
Published: July 2020

The article presents methods of securing mining excavations using wooden cribs. For the underground room and pillar method used to excavate zinc and lead ore body in the Olkusz-Pomorzany mine in Poland, model tests for the replacement of rock pillars by wooden cribs are presented. In the first stage of research, the results of laboratory strength tests carried out on models of four-point, six-point and eight-point cribs made of wooden beech beams at a 1:28 scale arranged horizontally were determined. For the first time, a concave round notch connection was used to connect the beams of the wooden cribs. The maximal capacity of cribs consisting only of beams and filled with waste rocks taken from underground mining excavations was determined. In addition, the vertical deformations of the cribs at maximal loading force and their specific deformations are presented. Additionally, on the basis of load-displacement characteristics, the range in variability of the stiffness of empty cribs and those filled with waste rocks was calculated as a function of their compressibility. In the second stage of research, the room and pillar method was designed in the Phase2 numerical program. The aim of the study was to determine the stresses in the inter-room pillars. Based on the results of laboratory and numerical tests, a factor of safety was determined, indicating that it is possible to reduce mining losses while maintaining the safe exploitation conditions of the ore body.
Design of Low-Cost Synchronous Machine to Prevent Demagnetization
Authors: Bianchini Claudio , Torreggiani Ambra , Davoli Matteo , Bellini Alberto
Published: July 2020

The request for high efficiency motor paves the way for the replacement of induction motors with permanent magnet synchronous motors. Although the efficiency is increased, for medium and high power, the current ripple causes significant additional losses in the magnet and lamination; and, high temperature can lead to demagnetization. In this paper, a new rotor topology is proposed and compared to a traditional surface permanent magnet rotor to reduce the magnet losses and protect them from demagnetization. A reference surface permanent magnet machine is compared with the proposed one in terms of performance and magnet losses. Both analytical and experimental analysis are carried out and discussed.
Dielectric Properties and Dipole Moment of Edible Oils Subjected to ‘Frying’ Thermal Treatment
Authors: Šegatin Nataša , Pajk Žontar Tanja , Poklar Ulrih Nataša
Published: July 2020

The dielectric properties of six refined edible oils with different fatty-acid compositions were determined for oils incubated at 180 °C up to 40 h. The oil degradation was evaluated by the dielectric dispersion and dielectric loss in the frequency range from 40 Hz to 2 MHz at 25 °C, refractive index, density, saponification number, and specific absorption coefficient at 232 and 268 nm. The dependence of the dielectric properties on frequency has been evaluated with Corach, Cole–Cole, and the universal power law models, giving the novel strategies for the interpretation of the dielectric spectra of thermally treated oils. The derived parameters—the dielectric constant, the electrical conductivity, the relaxation time τ and the exponents α, p, and n—are discussed with respect to the increased oxidation evidenced by specific absorption coefficients and polar products, as measured by the dielectric constant of the thermally treated oils. The specific refraction, specific polarization, orientation polarization, and dipole moment were determined using Lorenz–Lorentz, Debye and Onsager relationship. All above parameters obtained increased during the thermal treatment, except specific refraction, the electrical conductivity and the relaxation time. The dielectric constant-macroscopic parameter was compared with microscopic parameter polarization and dipole moment; the linear dependence was found to be R 2 = 0.971 .
Physiochemical Characteristics of Hot and Cold Brew Coffee Chemistry: The Effects of Roast Level and Brewing Temperature on Compound Extraction
Authors: Rao Niny Z. , Fuller Megan , Grim Meghan D.
Published: July 2020

The role of roasting in cold brew coffee chemistry is poorly understood. The brewing temperature influences extraction processes and may have varying effects across the roast spectrum. To understand the relationship between brew temperature and roast temperature, hot and cold brew coffees were prepared from Arabica Columbian coffee beans roasted to light, medium, and dark levels. Chemical and physical parameters were measured to investigate the relationships among degree of roast, water temperature, and key characteristics of resulting coffees. Cold brew coffees showed differential extraction marked by decreased acidity, lower concentration of browned compounds, and fewer TDS indicating that cold water brewing extracts some compounds less effectively than hot water brewing. Compounds in coffee did exhibit sensitivity to degree of roast, with darker roasts resulting in decreased concentrations for both hot and cold brew coffees. Total antioxidant capacity (TAC) was only sensitive to degree of roast in cold brew coffees, while hot brew coffees had a constant TAC for all three roast levels. This indicates that the solid bean matrix and its chemical constituents interact with cold water differently than with hot water. Surface wetting, pore dynamics, and solubility all contribute to the extraction potential during brewing and are all functions of water temperature.
Snack Development for School Feeding Programs in Africa: A Scoping Review
Authors: Hassanally Saffia , Naicker Ashika , Singh Evonne
Published: July 2020

The benefits of school feeding have been well documented. As such, school feeding programs have continuously gained popularity in developing countries. However, challenges and potential opportunities persist, calling for a review of school feeding for long-term sustainability. South Africa has an opportunity to improve their National School Nutrition Program (NSNP) by including an energy-dense snack that would increase the recommended dietary allowance (RDA) of school children to meet at least 25% of their energy requirements. The objective of this scoping review was to conduct a review and an appraisal of studies on snack food development for school feeding programs in Africa. Eligible studies had to report snack development for school feeding programs in Africa. We conducted an electronic search in National Research Foundation (NRF) NEXUS, Elton B. Stephens Company (EBSCO), International Food Information Service (IFIS), Nutrition and Food Sciences Center for Agriculture and Bioscience International (CABI.ORG), and Google Scholar. Of the 429 articles identified, nine studies were included in the final review—five from within South Africa and four from elsewhere in Africa. Data extracted included the study design, intervention, outcomes, relevant findings, and limitations. Results were presented in a narrative summary. The review findings showed that energy-rich staple foods and food fortification were commonly used in snack development. The popular snack products developed included porridges and biscuits. While most studies reported nutritional outcomes, few studies reported on sensory acceptability tests and only two studies conducted a cost analysis. This review of previous snack development initiatives for school feeding programs in Africa underscores the importance of establishing the sustainability of any food product developed. The findings of this review have the potential to inform future snack product development for school feeding programs.
Discovery of Novel Non-Steroidal Cytochrome P450 17A1 Inhibitors as Potential Prostate Cancer Agents
Authors: Wróbel Tomasz M. , Rogova Oksana , Andersen Kasper L. , Yadav Rahul , Brixius-Anderko Simone , Scott Emily E. , Olsen Lars , Jørgensen Flemming Steen , Björkling Fredrik
Published: July 2020

The current study presents the design, synthesis, and evaluation of novel cytochrome P450 17A1 (CYP17A1) ligands. CYP17A1 is a key enzyme in the steroidogenic pathway that produces androgens among other steroids, and it is implicated in prostate cancer. The obtained compounds are potent enzyme inhibitors (sub µM) with antiproliferative activity in prostate cancer cell lines. The binding mode of these compounds is also discussed.
Recovery of Fatty Acid Composition in Mediterranean Yellowtail (Seriola dumerili, Risso 1810) fed a Fish-Oil Finishing Diet
Authors: Bordignon Francesco , Martínez-Llorens Silvia , Trocino Angela , Jover-Cerdá Miguel , Tomás-Vidal Ana
Published: July 2020

The present study evaluated the effects of wash-out on the fatty acid (FA) composition in the muscles of Mediterranean yellowtail. After 109 days during which fish were fed either a fish oil (FO)-based diet (FO 100) or a diet (FO 0) in which FO was completely substituted by vegetable oils, all fish were subjected to a wash-out with FO 100 diet for 90 days. The FA profile of muscles in fish fed FO 0 diet at the beginning of the experiment reflected that of dietary vegetable oils, rich in linoleic acid (LA), and α-linolenic acid (ALA), and was deficient in AA (arachidonic acid), EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid). No essential FA were fully restored in fish previously fed FO 0 diet on 45th or 90th day of wash-out. At the end of wash-out, the FA composition showed that AA, EPA, and DHA in the white muscles increased by +33%, +16%, and +43% (p < 0.001), respectively. Similarly, AA and DHA in the red muscles increased by +33% and +41% respectively, while EPA remained similar to fish fed FO 0 diet exclusively. Therefore, a 90-d wash-out can partially improve the FA profile in muscles of Mediterranean yellowtail previously fed vegetable oil-based diets.
Changes in Expression Level of OsHKT1;5 Alters Activity of Membrane Transporters Involved in K+ and Ca2+ Acquisition and Homeostasis in Salinized Rice Roots
Authors: Alnayef Mohammad , Solis Celymar , Shabala Lana , Ogura Takaaki , Chen Zhonghua , Bose Jayakumar , Maathuis Frans J. M. , Venkataraman Gayatri , Tanoi Keitaro , Yu Min , Zhou Meixue , Horie Tomoaki , Shabala Sergey
Published: July 2020

In rice, the OsHKT1;5 gene has been reported to be a critical determinant of salt tolerance. This gene is harbored by the SKC1 locus, and its role was attributed to Na+ unloading from the xylem. No direct evidence, however, was provided in previous studies. Also, the reported function of SKC1 on the loading and delivery of K+ to the shoot remains to be explained. In this work, we used an electrophysiological approach to compare the kinetics of Na+ uptake by root xylem parenchyma cells using wild type (WT) and NIL(SKC1) plants. Our data showed that Na+ reabsorption was observed in WT, but not NIL(SKC1) plants, thus questioning the functional role of HKT1;5 as a transporter operating in the direct Na+ removal from the xylem. Instead, changes in the expression level of HKT1;5 altered the activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in the rice epidermis and stele, explaining the observed phenotype. We conclude that the role of HKT1;5 in plant salinity tolerance cannot be attributed to merely reducing Na+ concentration in the xylem sap but triggers a complex feedback regulation of activities of other transporters involved in the maintenance of plant ionic homeostasis and signaling under stress conditions.
A Comprehensive Machine-Learning Model Applied to Magnetic Resonance Imaging (MRI) to Predict Alzheimer’s Disease (AD) in Older Subjects
Authors: Battineni Gopi , Chintalapudi Nalini , Amenta Francesco , Traini Enea
Published: July 2020

Increasing evidence suggests the utility of magnetic resonance imaging (MRI) as an important technique for the diagnosis of Alzheimer’s disease (AD) and for predicting the onset of this neurodegenerative disorder. In this study, we present a sophisticated machine learning (ML) model of great accuracy to diagnose the early stages of AD. A total of 373 MRI tests belonging to 150 subjects (age ≥ 60) were examined and analyzed in parallel with fourteen distinct features related to standard AD diagnosis. Four ML models, such as naive Bayes (NB), artificial neural networks (ANN), K-nearest neighbor (KNN), and support-vector machines (SVM), and the receiver operating characteristic (ROC) curve metric were used to validate the model performance. Each model evaluation was done in three independent experiments. In the first experiment, a manual feature selection was used for model training, and ANN generated the highest accuracy in terms of ROC (0.812). In the second experiment, automatic feature selection was conducted by wrapping methods, and the NB achieved the highest ROC of 0.942. The last experiment consisted of an ensemble or hybrid modeling developed to combine the four models. This approach resulted in an improved accuracy ROC of 0.991. We conclude that the involvement of ensemble modeling, coupled with selective features, can predict with better accuracy the development of AD at an early stage.
Sequential Strategy Including FFRCT Plus Stress-CTP Impacts on Management of Patients with Stable Chest Pain: The Stress-CTP RIPCORD Study
Authors: Baggiano Andrea , Fusini Laura , Del Torto Alberico , Vivona Patrizia , Guglielmo Marco , Muscogiuri Giuseppe , Soldi Margherita , Martini Chiara , Fraschini Enrico , Rabbat Mark G. , Baessato Francesca , Cicala Gloria , Danza Maria L. , Cavaliere Annachiara , Loffreno Antonella , Palmisano Vitanio , Ricci Francesca , Rizzon Giulia , Tonet Elisabetta , Viani Giacomo M. , Mushtaq Saima , Conte Edoardo , Annoni Andrea D. , Formenti Alberto , Mancini Maria E. , Fabbiocchi Franco , Montorsi Piero , Trabattoni Daniela , Rossi Alexia , Fazzari Fabio , Gaibazzi Nicola , Andreini Daniele , Assanelli Emilio M. , Bartorelli Antonio L. , Pepi Mauro , Guaricci Andrea I. , Pontone Gianluca
Published: July 2020

Stress computed tomography perfusion (Stress-CTP) and computed tomography-derived fractional flow reserve (FFRCT) are functional techniques that can be added to coronary computed tomography angiography (cCTA) to improve the management of patients with suspected coronary artery disease (CAD). This retrospective analysis from the PERFECTION study aims to assess the impact of their availability on the management of patients with suspected CAD scheduled for invasive coronary angiography (ICA) and invasive FFR. The management plan was defined as optimal medical therapy (OMT) or revascularization and was recorded for the following strategies: cCTA alone, cCTA+FFRCT, cCTA+Stress-CTP and cCTA+FFRCT+Stress-CTP. In 291 prospectively enrolled patients, cCTA+FFRCT, cCTA+Stress-CTP and cCTA+FFRCT+Stress-CTP showed a similar rate of reclassification of cCTA findings when FFRCT and Stress-CTP were added to cCTA. cCTA, cCTA+FFRCT, cCTA+Stress-CTP and cCTA+FFRCT+Stress-CTP showed a rate of agreement versus the final therapeutic decision of 63%, 71%, 89%, 84% (cCTA+Stress-CTP and cCTA+FFRCT+Stress-CTP vs cCTA and cCTA+FFRCT: p < 0.01), respectively, and a rate of agreement in terms of the vessels to be revascularized of 57%, 64%, 74%, 71% (cCTA+Stress-CTP and cCTA+FFRCT+Stress-CTP vs cCTA and cCTA+FFRCT: p < 0.01), respectively, with an effective radiation dose (ED) of 2.9 ± 1.3 mSv, 2.9 ± 1.3 mSv, 5.9 ± 2.7 mSv, and 3.1 ± 2.1 mSv. The addition of FFRCT and Stress-CTP improved therapeutic decision-making compared to cCTA alone, and a sequential strategy with cCTA+FFRCT+Stress-CTP represents the best compromise in terms of clinical impact and radiation exposure.
Association of Hypertensive Intracerebral Hemorrhage with Left Ventricular Hypertrophy on Transthoracic Echocardiography
Authors: Pallesen Lars-Peder , Wagner Jenny , Lambrou Dimitris , Braun Silke , Weise Matthias , Prakapenia Alexandra , Barlinn Jessica , Siepmann Timo , Winzer Simon , Moustafa Haidar , Kitzler Hagen H. , Barlinn Kristian , Reichmann Heinz , Puetz Volker
Published: July 2020

Introduction: Arterial hypertension is the most frequent cause for spontaneous intracerebral hemorrhage (sICH) and may also cause left ventricular hypertrophy (LVH). We sought to analyze whether hypertensive sICH etiology is associated with LVH. Methods: We analyzed consecutive patients with sICH who were admitted to our tertiary stroke center during a four-year period and underwent transthoracic echocardiography (TTE) as part of the diagnostic work-up. We defined hypertensive sICH as typical localization of hemorrhage in patients with arterial hypertension and no other identified sICH etiology. We defined an increased end-diastolic interventricular septal wall thickness of ≥11 mm on TTE as a surrogate parameter for LVH. Results: Among 395 patients with sICH, 260 patients (65.8%) received TTE as part of their diagnostic work-up. The median age was 71 years (interquartile range (IQR) 17), 160 patients (61.5%) were male, the median baseline National Institute of Health Stroke Scale (NIHSS) score was 8 (IQR 13). Of these, 159 (61.2%) patients had a hypertensive sICH and 156 patients (60%) had LVH. In univariable (113/159 (71.1%) vs. 43/101 (42.6%); odds ratio (OR) 3.31; 95% confidence interval (CI95%) 1.97–5.62); and multivariable (adjusted OR 2.95; CI95% 1.29–6.74) analysis, hypertensive sICH was associated with LVH. Conclusions: In patients with sICH, LVH is associated with hypertensive bleeding etiology. Performing TTE is meaningful for diagnosis of comorbidities and clarification of bleeding etiology in these patients. Future studies should include long-term outcome parameters and assess left ventricular mass as main indicator for LVH.
The Impact of Dysphagia in Myositis: A Systematic Review and Meta-Analysis
Authors: Labeit Bendix , Pawlitzki Marc , Ruck Tobias , Muhle Paul , Claus Inga , Suntrup-Krueger Sonja , Warnecke Tobias , Meuth Sven G. , Wiendl Heinz , Dziewas Rainer
Published: July 2020

(1) Background: Dysphagia is a clinical hallmark and part of the current American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) diagnostic criteria for idiopathic inflammatory myopathy (IIM). However, the data on dysphagia in IIM are heterogenous and partly conflicting. The aim of this study was to conduct a systematic review on epidemiology, pathophysiology, outcome and therapy and a meta-analysis on the prevalence of dysphagia in IIM. (2) Methods: Medline was systematically searched for all relevant articles. A random effect model was chosen to estimate the pooled prevalence of dysphagia in the overall cohort of patients with IIM and in different subgroups. (3) Results: 234 studies were included in the review and 116 (10,382 subjects) in the meta-analysis. Dysphagia can occur as initial or sole symptom. The overall pooled prevalence estimate in IIM was 36% and with 56% particularly high in inclusion body myositis. The prevalence estimate was significantly higher in patients with cancer-associated myositis and with NXP2 autoantibodies. Dysphagia is caused by inflammatory involvement of the swallowing muscles, which can lead to reduced pharyngeal contractility, cricopharyngeal dysfunction, reduced laryngeal elevation and hypomotility of the esophagus. Swallowing disorders not only impair the quality of life but can lead to serious complications such as aspiration pneumonia, thus increasing mortality. Beneficial treatment approaches reported include immunomodulatory therapy, the treatment of associated malignant diseases or interventional procedures targeting the cricopharyngeal muscle such as myotomy, dilatation or botulinum toxin injections. (4) Conclusion: Dysphagia should be included as a therapeutic target, especially in the outlined high-risk groups.
Epileptiform Discharge and Electrographic Seizures during the Hypothermia Phase as Predictors of Rewarming Seizures in Children after Resuscitation
Authors: Lin Jainn-Jim , Hsu Mei-Hsin , Hsia Shao-Hsuan , Lin Ying-Jui , Wang Huei-Shyong , Kuo Hsuan-Chang , Chiang Ming-Chou , Chan Oi-Wa , Lee En-Pei , Lin Kuang-Lin ,   the iCNS Group
Published: July 2020

The aim of this study was to determine the frequency, timing, and predictors of rewarming seizures in a cohort of children undergoing therapeutic hypothermia after resuscitation. We retrospectively reviewed consecutive pediatric patients undergoing therapeutic hypothermia after resuscitation admitted to our pediatric intensive care unit between January 2000 and December 2019. Continuous electroencephalographic monitoring was performed during hypothermia (24 h for cardiac aetiologies and 72 h for asphyxial aetiologies), rewarming (72 h), and then an additional 12 h of normothermia. Thirty comatose children undergoing therapeutic hypothermia after resuscitation were enrolled, of whom 10 (33.3%) had rewarming seizures. Two (20%) of these patients had their first seizure during the rewarming phase. Four (40%) patients had electroclinical seizures, and six (60%) had nonconvulsive seizures. The median time from starting rewarming to the onset of rewarming seizures was 37.3 h (range 6 to 65 h). The patients with interictal epileptiform activity and electrographic seizures during the hypothermia phase were more likely to have rewarming seizures compared to those without interictal epileptiform activity or electrographic seizures (p = 0.019 and 0.019, respectively). Therefore, in high-risk patients, continuous electroencephalographic monitoring for a longer duration may help to detect rewarming seizures and guide clinical management.
Autism Spectrum Disorders in Greece: Nationwide Prevalence in 10–11 Year-Old Children and Regional Disparities
Authors: Thomaidis Loretta , Mavroeidi Nikoletta , Richardson Clive , Choleva Antigoni , Damianos George , Bolias Konstantinos , Tsolia Maria
Published: July 2020

Autism spectrum disorders (ASD) constitute a public health concern with increasing prevalence worldwide. We aimed to estimate prevalence and age at diagnosis in Greece, where no large-scale prevalence study has ever been conducted. Aggregate data were collected on ASD diagnoses by gender and calendar year of diagnosis up to 2019, for children born in 2008 and 2009, from the Centers for Educational and Counseling Support, which evaluate children to receive special educational support in school. Coverage was 87.1% of centers and 88.1% of schoolchildren born in 2008–9. ASD prevalence overall was 1.15% (1.83% males, 0.44% females; ratio 4.14:1), ranging from 0.59% to 1.50% in Greece’s 13 regions. In five regions, prevalence differed significantly between centers. Overall, only 3.8% of diagnoses were made before the fourth year after birth and 42.7% before the sixth year, with considerable variation between regions. Approximate mean age at diagnosis was six years and one month, and about three months earlier for girls than for boys. Our results provide evidence-based information to guide service planning and development at national and regional levels. Particular attention should be paid to smoothing out inequalities regarding service accessibility and provision. Emphasis should be given to earlier identification and diagnosis of ASD.
Delirium Post-Stroke—Influence on Post-Stroke Dementia (Research Study—Part of the PROPOLIS Study)
Authors: Droś Jakub , Kowalska Katarzyna , Pasińska Paulina , Szyper-Maciejowska Aleksandra , Gorzkowska Agnieszka , Klimkowicz-Mrowiec Aleksandra
Published: July 2020

Background: Previous research confirmed association between delirium and subsequent dementia in different clinical settings, but the impact of post-stroke delirium on cognitive functioning is still under-investigated. Therefore, we aimed to assess the risk of dementia among patients with stroke and in-hospital delirium. Methods: A total of 750 consecutive patients admitted to the stroke unit with acute stroke or transient ischemic attacks were screened for delirium, during the first seven days after admission. At the three- and twelve-month follow-up, patients underwent cognitive evaluation. The DSM-5 definition for dementia was used. Cases with pre-stroke dementia were excluded from the analysis. Results: Out of 691 included cases, 423 (61.22%) and 451 (65.27%) underwent cognitive evaluation, three and twelve months after stroke; 121 (28.61%) and 151 (33.48%) patients were diagnosed with dementia, respectively. Six (4.96%) patients with dementia, three months post-stroke did not meet the diagnostic criteria for dementia nine months later. After twelve months, 37 (24.50%) patients were diagnosed with dementia, first time after stroke. Delirium in hospital was an independent risk factor for dementia after three months (OR = 7.267, 95%CI 2.182–24.207, p = 0.001) but not twelve months after the stroke. Conclusions: Patients with stroke complicated by in-hospital delirium are at a higher risk for dementia at three but not twelve months post-stroke.
The Uncertain Effect of Antimicrobial Therapy in the Treatment of Patients with Ischemic Colitis
Authors: Lee Jae Gon , Park Jin Hwa , Han Dong Soo , Lee Hang Lak , Park Chan Hyuk , Eun Chang Soo
Published: July 2020

Although antimicrobial therapy is recommended for patients with moderate or severe ischemic colitis, its beneficial effects are unclear. In the present study, the role of antimicrobial therapy in the treatment of ischemic colitis was investigated. Patients with ischemic colitis were retrospectively identified between January 2004 and June 2019. The characteristics and outcomes of patients who received antibiotics (antibiotics group) and those who did not (no-antibiotics group) were compared. Clinical outcomes included death, surgery, and readmission within 30 days, fasting duration, and hospital stay. Data from 186 patients were analyzed; 122 patients were in the antibiotics group and 64 in the no-antibiotics group. Composite outcome of death, surgery, and readmission within 30 days occurred in 3.3% of patients in the antibiotics group and 3.1% of patients in the no-antibiotics group (p > 0.999). Fasting duration was not significantly different between the two groups (median days, 4.0 vs. 4.0, p = 0.253). However, hospital stays were longer in the antibiotics group than in the no-antibiotics group (median days, 9.0 vs. 7.0, p = 0.043). In patients with ischemic colitis, there was no statistically significant difference in the incidence of death, surgery, and readmission within 30 days between patients who received antibiotics and those who did not receive antibiotics.
Early Pregnancy Vitamin D Binding Protein Is Independently Associated with the Development of Gestational Diabetes: A Retrospective Cohort Study
Authors: Fernando Melinda , Ellery Stacey J. , de Guingand Deborah , Marquina Clara , Lim Siew , Harrison Cheryce L. , Teede Helena J. , Naderpoor Negar , Mousa Aya
Published: July 2020

Background: Vitamin D-binding protein (VDBP) has been implicated in several adverse pregnancy outcomes either directly or indirectly via influencing the concentrations of biologically active vitamin D metabolites. However, human studies exploring these metabolites in pregnancy remain sparse. Here, we examine whether VDBP and total, free, and bioavailable 25-hydroxyvitamin D (25(OH)D) metabolites in early pregnancy are associated with subsequent adverse pregnancy outcomes. Methods: We conducted a retrospective analysis of 304 pregnant women in early pregnancy (<20 weeks gestation). The demographic characteristics, anthropometric data, and total 25(OH)D were measured and plasma or serum samples were collected and bio-banked. Using these samples, we measured VDBP (polyclonal ELISA) and albumin (automated colorimetry), and calculated free and bioavailable 25(OH)D using validated formulae. Pregnancy outcomes were derived from scanned medical records. Regression models were used to analyse the relationships between vitamin D metabolites in early pregnancy and subsequent pregnancy outcomes (gestational diabetes mellitus (GDM), pre-eclampsia, preterm birth), with adjustment for predetermined clinically relevant maternal factors including age, body mass index (BMI), and ethnicity. Results: Lower VDBP concentrations were associated with higher glucose levels and a greater likelihood of developing GDM at 26–28 weeks gestation (odds ratio [OR] (95% CI) = 0.98 (0.97,0.99), p = 0.015). This finding remained significant after adjustment for maternal covariates including age, BMI, and ethnicity (β = −0.003, p = 0.03). Lower total, free and bioavailable 25(OH)D, but not VDBP, were associated with a shorter length of gestation, but only the relationship with total 25(OH)D remained significant after adjustment for the above maternal covariates (β = 0.02, p = 0.006). Conclusions: This is the first study to examine VDBP, and total, free and bioavailable 25(OH)D in relation to pregnancy outcomes in a well characterised multi-ethnic cohort of pregnant women. Our findings show that VDBP and total 25(OH)D are associated with GDM and length of gestation, respectively; however, further investigations using large-scale prospective studies are needed to confirm our findings.
The Contribution of Forerunner to Storm Surges along the Vietnam Coast
Authors: Trinh Tam Thi , Pattiaratchi Charitha , Bui Toan
Published: July 2020

Vietnam, located in the tropical region of the northwest Pacific Ocean, is frequently impacted by tropical storms. Occurrence of extreme water level events associated with tropical storms are often unpredicted and put coastal infrastructure and safety of coastal populations at risk. Hence, an improved understanding of the nature of storm surges and their components along the Vietnam coast is required. For example, a higher than expected extreme storm surge during Typhoon Kalmegi (2014) highlighted the lack of understanding on the characteristics of storm surges in Vietnam. Physical processes that influence the non-tidal water level associated with tropical storms can persist for up to 14 days, beginning 3–4 days prior to storm landfall and cease up to 10 days after the landfall of the typhoon. This includes the forerunner, ‘direct’ storm surge, and coastally trapped waves. This study used a continuous record of six sea level time series collected over a 5-year period (2013–2017) from along the Vietnam coast and Hong Kong to examine the contribution of the forerunner to non-tidal water level. The forerunner is defined as the gradual increase in mean water level, 2–3 days prior to typhoon landfall and generated by shore parallel winds and currents that result in a mean higher water level at the coast. Results indicated that a forerunner was generated by almost all typhoons, at least at one station, with a range between 20 and 50 cm. The forerunner contributed up to 50% of the water level change due to the storm. Combination of forerunner and onshore winds generated storm surges that were much higher (to 70 cm). It was also found that the characteristics of the typhoon (e.g., path, speed, severity and size) significantly influenced the generation of the forerunner. It is recommended that the forerunner that is not currently well defined in predictive models should be included in storm surge forecasts.