bluuebunny/crossref_metadata_2025 huggingface dataset:
Alterations of Gut Microbiota in Patients With Graves’ Disease
Authors: Chang Shih-Cheng , Lin Shu-Fu , Chen Szu-Tah , Chang Pi-Yueh , Yeh Yuan-Ming , Lo Fu-Sung , Lu Jang-Jih
Published: May 2021

Graves’ disease (GD) is a systemic autoimmune disease characterized by hyperthyroidism. Evidence suggests that alterations to the gut microbiota may be involved in the development of autoimmune disorders. The aim of this study was to characterize the composition of gut microbiota in GD patients. Fecal samples were collected from 55 GD patients and 48 healthy controls. Using 16S rRNA gene amplification and sequencing, the overall bacterial richness and diversity were found to be similar between GD patients and healthy controls. However, principal coordinate analysis and partial least squares-discriminant analysis showed that the overall gut microbiota composition was significantly different (ANOSIM; p < 0.001). The linear discriminant analysis effect size revealed that Firmicutes phylum decreased in GD patients, with a corresponding increase in Bacteroidetes phylum compared to healthy controls. In addition, the families Prevotellaceae, and Veillonellaceae and the genus Prevotella_9 were closely associated with GD patients, while the families Lachnospiraceae and Ruminococcaceae and the genera Faecalibacterium, Lachnospira, and Lachnospiraceae NK4A136 were associated with healthy controls. Metagenomic profiles analysis yielded 22 statistically significant bacterial taxa: 18 taxa were increased and 4 taxa were decreased. Key bacterial taxa with different abundances between the two groups were strongly correlated with GD-associated clinical parameters using Spearman’s correlation analysis. Importantly, the discriminant model based on predominant microbiota could effectively distinguish GD patients from healthy controls (AUC = 0.825). Thus, the gut microbiota composition between GD patients and healthy controls is significantly difference, indicating that gut microbiota may play a role in the pathogenesis of GD. Further studies are needed to fully elucidate the role of gut microbiota in the development of GD.
Sensitivity of Iodine-Mediated Stratospheric Ozone Loss Chemistry to Future Chemistry-Climate Scenarios
Authors: Klobas J. Eric , Hansen Janina , Weisenstein Debra K. , Kennedy Robert P. , Wilmouth David M.
Published: May 2021

As the chemical and physical state of the stratosphere evolves, so too will the rates of important ozone-destroying reactions. In this work, we evaluate the chemistry-climate sensitivity of reactions of stratospheric iodine, reporting the iodine alpha factor (the efficiency of ozone loss mediated by a single iodine atom relative to the ozone loss mediated by a single chlorine atom) and the iodine eta factor (the efficiency of ozone loss mediated by a single iodine atom relative to the ozone loss mediated by a single chlorine atom in a benchmark chemistry-climate state) as a function of future greenhouse gas emissions scenario. We find that iodine-mediated ozone loss is much less sensitive to future changes in the state of the stratosphere than chlorine- and bromine-mediated reactions. Additionally, we demonstrate that the inclusion of the heterogeneous reaction of ozone with aqueous iodide in stratospheric aerosol produces substantial enhancements in the iodine alpha and eta factors relative to evaluations that consider gas-phase iodine reactions only. We conclude that the share of halogen-induced ozone loss due to reactions of iodine will likely be greater in the future stratosphere than it is today.
Metabolic Plasticity of Gilthead Seabream Under Different Stressors: Analysis of the Stress Responsive Hepatic Proteome and Gene Expression
Authors: Raposo de Magalhães Cláudia , Schrama Denise , Nakharuthai Chatsirin , Boonanuntanasarn Surintorn , Revets Dominique , Planchon Sébastien , Kuehn Annette , Cerqueira Marco , Carrilho Raquel , Farinha Ana Paula , Rodrigues Pedro M.
Published: May 2021

Hepatic metabolic adjustments are key adaptive mechanisms to stress in fish targeting at increasing energy availability for the animal to efficiently cope with a stressor. Teleosts exhibit a broad variety of these metabolic responses, depending on the species biology, individual experiences and the challenge’s characteristics. Nevertheless, the molecular response to a prolonged stress can be more heterogeneous and far more complex to interpret than that to an acute stress. A comparative proteomics analysis was employed to discover the set of liver proteins involved in the adaptive processes that tune the physiological response ofSparus auratato different suboptimal rearing conditions and physical challenges. Three separated trials were established where fish were submitted to different conditions (overcrowding, net handling and hypoxia). The response at the transcript level of 13 genes was also assessed. Mass spectrometric analysis revealed 71 differential abundant proteins distributed among the trials. Prolonged exposure to stress seems to have induced widespread changes in amino acid, carbohydrate, and lipid metabolisms, antioxidant response and protein folding, sorting and degradation processes. Two genes corresponding to heat-shock proteins were found to be differently expressed in net handled fish. These results shed light on the dynamics and extent of this species’ metabolic reprogramming under different challenges, supporting future studies on stress markers’ discovery and fish welfare research.
Case Report: Arthroscopic “Debridement Antibiotics and Implant Retention” With Local Injection of Personalized Phage Therapy to Salvage a Relapsing Pseudomonas Aeruginosa Prosthetic Knee Infection
Authors: Ferry Tristan , Kolenda Camille , Batailler Cécile , Gaillard Romain , Gustave Claude-Alexandre , Lustig Sébastien , Fevre Cindy , Petitjean Charlotte , Leboucher Gilles , Laurent Frédéric ,   The Lyon BJI Study group
Published: May 2021

Bacteriophages are viruses that specifically target bacteria. They are considered to have a high potential in patients with prosthetic joint infection (PJI), as they have a synergistic anti-biofilm activity with antibiotics. We report here the case of an 88-year-old man (63 kg) with relapsing Pseudomonas aeruginosa prosthetic knee infection. The patient had severe alteration of the general status and was bedridden with congestive heart failure. As prosthesis explantation and/or exchange was not feasible, we proposed to this patient the use of phage therapy to try to control the disease in accordance with the local ethics committee and the French National Agency for Medicines and Health Products Safety (ANSM). Three phages, targeting P. aeruginosa, were selected based on their lytic activity on the patient's strain (phagogram). Hospital pharmacist mixed extemporaneously the active phages (initial concentration 1 ml of 1 × 1010 PFU/ml for each phage) to obtain a cocktail of phages in a suspension form (final dilution 1 × 109 PFU/ml for both phages). Conventional arthroscopy was performed and 30 cc of the magistral preparation was injected through the arthroscope (PhagoDAIR procedure). The patient received intravenous ceftazidime and then oral ciprofloxacin as suppressive antimicrobial therapy. Under this treatment, the patient rapidly improved with disappearance of signs of heart failure and pain of the left knee. During the follow-up of 1 year, the local status of the left knee was normal, and its motion and walking were unpainful. The present case suggests that the PhagoDAIR procedure by arthroscopy has the potential to be used as salvage therapy for patients with P. aeruginosa relapsing PJI, in combination with suppressive antimicrobial therapy. A Phase II clinical study deserves to be performed to confirm this hypothesis.
Bacterial Communities Associated With Four Blooming Scyphozoan Jellyfish: Potential Species-Specific Consequences for Marine Organisms and Humans Health
Authors: Peng Saijun , Hao Wenjin , Li Yongxue , Wang Lei , Sun Tingting , Zhao Jianmin , Dong Zhijun
Published: May 2021

Cnidarians have large surface areas available for colonization by microbial organisms, which serve a multitude of functions in the environment. However, relatively few studies have been conducted on scyphozoan-associated microbial communities. Blooms of scyphozoan species are common worldwide and can have numerous deleterious consequences on the marine ecosystem. Four scyphozoan species, Aurelia coerulea, Cyanea nozakii, Nemopilema nomurai, and Rhopilema esculentum, form large blooms in Chinese seas. In this study, we analyzed the bacterial communities associated with these four jellyfish based on 16S rRNA gene sequencing. We found that the bacterial communities associated with each scyphozoan species were significantly different from each other and from those of the surrounding seawater. There were no significant differences between the bacterial communities associated with different body parts of the four scyphozoan jellyfish. Core bacteria in various compartments of the four scyphozoan taxa comprised 57 OTUs (Operational Taxonomic Units), dominated by genera Mycoplasma, Vibrio, Ralstonia, Tenacibaculum, Shingomonas and Phyllobacterium. FAPROTAX function prediction revealed that jellyfish could influence microbially mediated biogeochemical cycles, compound degradation and transmit pathogens in regions where they proliferate. Finally, Six genera of potentially pathogenic bacteria associated with the scyphozoans were detected: Vibrio, Mycoplasma, Ralstonia, Tenacibaculum, Nautella, and Acinetobacter. Our study suggests that blooms of these four common scyphozoans may cause jellyfish species-specific impacts on element cycling in marine ecosystems, and serve as vectors of pathogenic bacteria to threaten other marine organisms and human health.
Development of the Entorhinal Cortex Occurs via Parallel Lamination During Neurogenesis
Authors: Liu Yong , Bergmann Tobias , Mori Yuki , Peralvo Vidal Juan Miguel , Pihl Maria , Vasistha Navneet A. , Thomsen Preben Dybdahl , Seemann Stefan E. , Gorodkin Jan , Hyttel Poul , Khodosevich Konstantin , Witter Menno P. , Hall Vanessa Jane
Published: May 2021

The entorhinal cortex (EC) is the spatial processing center of the brain and structurally is an interface between the three layered paleocortex and six layered neocortex, known as the periarchicortex. Limited studies indicate peculiarities in the formation of the EC such as early emergence of cells in layers (L) II and late deposition of LIII, as well as divergence in the timing of maturation of cell types in the superficial layers. In this study, we examine developmental events in the entorhinal cortex using an understudied model in neuroanatomy and development, the pig and supplement the research with BrdU labeling in the developing mouse EC. We determine the pig serves as an excellent anatomical model for studying human neurogenesis, given its long gestational length, presence of a moderate sized outer subventricular zone and early cessation of neurogenesis during gestation. Immunohistochemistry identified prominent clusters of OLIG2+ oligoprogenitor-like cells in the superficial layers of the lateral EC (LEC) that are sparser in the medial EC (MEC). These are first detected in the subplate during the early second trimester. MRI analyses reveal an acceleration of EC growth at the end of the second trimester. BrdU labeling of the developing MEC, shows the deeper layers form first and prior to the superficial layers, but the LV/VI emerges in parallel and the LII/III emerges later, but also in parallel. We coin this lamination pattern parallel lamination. The early born Reln+ stellate cells in the superficial layers express the classic LV marker, Bcl11b (Ctip2) and arise from a common progenitor that forms the late deep layer LV neurons. In summary, we characterize the developing EC in a novel animal model and outline in detail the formation of the EC. We further provide insight into how the periarchicortex forms in the brain, which differs remarkably to the inside-out lamination of the neocortex.
The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina
Authors: Wang Qin , Banerjee Seema , So ChungHim , Qiu ChunTing , Sze YingHon , Lam Thomas Chuen , To Chi-Ho , Pan Feng
Published: May 2021

Low-dose atropine helps to control myopia progression with few side effects. However, the impact of atropine, a non-selective muscarinic Acetylcholine (ACh) receptor antagonist, on retinal ganglion cells (RGCs) remains unclear. After immersing the cornea and adjacent conjunctiva of enucleated eyes in 0.05% (approximately 800 μM) atropine solution for 30 min, the atropine concentration reached in the retina was below 2 μM. After direct superfusion of the retina with 1 μM atropine (considering that the clinical application of 0.05% atropine eye drops will be diluted over time due to tear flow for 30 min), no noticeable changes in the morphology of ON and OFF alpha RGCs (αRGCs) were observed. Atropine affected the light-evoked responses of ON and OFF αRGCs in a dose- and time-dependent fashion. Direct application of less than 100 μM atropine on the retina did not affect light-evoked responses. The time latency of light-induced responses of ON or OFF αRGCs did not change after the application of 0.05–100 μM atropine for 5 min. However, 50 μM atropine extended the threshold of joint inter-spike interval (ISI) distribution of the RGCs. These results indicated that low-dose atropine (<0.5 μM; equal to 1% atropine topical application) did not interfere with spike frequency, the pattern of synchronized firing between OFF αRGCs, or the threshold of joint ISI distribution of αRGCs. The application of atropine unmasked inhibition to induce ON responses from certain OFF RGCs, possiblyviathe GABAergic pathway, potentially affecting visual information processing.
Neurochemistry of Visual Attention
Authors: Lockhofen Denise Elfriede Liesa , Mulert Christoph
Published: May 2021

Visual attention is the cognitive process that mediates the selection of important information from the environment. This selection is usually controlled by bottom-up and top-down attentional biasing. Since for most humans vision is the dominant sense, visual attention is critically important for higher-order cognitive functions and related deficits are a core symptom of many neuropsychiatric and neurological disorders. Here, we summarize the importance and relative contributions of different neuromodulators and neurotransmitters to the neural mechanisms of top-down and bottom-up attentional control. We will not only review the roles of widely accepted neuromodulators, such as acetylcholine, dopamine and noradrenaline, but also the contributions of other modulatory substances. In doing so, we hope to shed some light on the current understanding of the role of neurochemistry in shaping neuron properties contributing to the allocation of attention in the visual field.
The Val66 and Met66 Alleles-Specific Expression of BDNF in Human Muscle and Their Metabolic Responsivity
Authors: de Assis Gilmara Gomes , Hoffman Jay R. , Bojakowski Jacek , Murawska-Ciałowicz Eugenia , Cięszczyk Paweł , Gasanov Eugene V.
Published: May 2021

Brain-derived neurotrophic factor (BDNF) plays an essential role in nervous system formation and functioning, including metabolism. Present only in humans, the “Val66Met” polymorphism of the BDNF gene (BDNF) is suggested to have a negative influence on the etiology of neurological diseases. However, this polymorphism has only been addressed, at the molecular level, in nonhuman models. Knowledge about Val66- and Met66-variant differences, to date, has been achieved at the protein level using either cell culture or animal models. Thus, the purpose of our study was to analyze the impact of the Val66Met polymorphism on BDNF expression in healthy humans and compare the allele-specific responses to metabolic stress. Muscle biopsies from 13 male recreational athletes (34 ± 9 years, 1.80 ± 0.08 m, 76.4 ± 10.5 kg) were obtained before and immediately following a VO2max test. Allele-specific BDNF mRNA concentrations were quantified by droplet digital PCR (ddPCR) in heterozygous and homozygous subjects. The results indicated that BDNF expression levels were influenced by the genotype according to the presence of the polymorphism. BDNF expression from the Met66-coding alleles, in heterozygotes, was 1.3-fold lower than that from the Val66-coding alleles. Total BDNF mRNA levels in these heterozygotes remained below the whole sample’s mean. A partial dominance was detected for the Val66-coding variant on the Met66-coding’s. BDNF expression levels decreased by an average of 1.8-fold following the VO2max test, independent of the individual’s genotype. The results of this study indicate that metabolic stress downregulates BDNF expression but not plasma BDNF concentrations. No correlation between expression level and plasma BDNF concentrations was found.
Real-Time In Situ Navigation System With Indocyanine Green Fluorescence for Sentinel Lymph Node Biopsy in Patients With Breast Cancer
Authors: Wang Zhaorui , Yang Xiaowei , Wang Jingjing , Liu Peng , Pan Yubo , Han Chunguang , Pei Jing
Published: May 2021

Background

The naked-eye invisibility of indocyanine green fluorescence limits the application of near-infrared fluorescence imaging (NIR) systems for real-time navigation during sentinel lymph node biopsy (SLNB) in patients with breast cancer undergoing surgery. This study aims to evaluate the effectiveness and safety of a novel NIR system in visualizing indocyanine green fluorescence images in the surgical field and the application value of combined methylene blue (MB) and the novel NIR system in SLNB.

Methods

Sixty patients with clinical node-negative breast cancer received indocyanine green (ICG) and MB as tracers. Two NIR system instruments, namely, lymphatic fluorescence imaging system (LFIS) designed by the University of Science and Technology of China and vascular imager by Langfang Mingde Medical Biotechnology Co., Ltd. (Langfang vascular imager), were used as navigation assistance to locate sentinel lymph nodes (SLNs). Excising the lymph nodes developed by both MB and ICG by two NIR systems or palpably suspicious as SLNs and undergoing rapid pathological examination.

Results

Both instruments exhibited 95% (57/60) success for real-time lymphatic fluorescent images. A total of 186 SLNs were identified, of which two were pathologically confirmed as lacking any lymph node tissue. SLN identification rate was 100% (184/184) for MB plus LFIS and 86.96% (160/184) for MB alone. The median number of SLNs identified by LFIS combined with MB was 3 (range of 1–8), which was significantly higher than that by MB alone at 2 (range 1–7) (P<0.05).

Conclusion

LFIS effectively detects SLNs in breast cancer, projects the fluorescence signals during surgery, and provides a continuous surgical navigation system without the need for a remote monitor. The ICG method navigated by combined LFIS and MB may be a promising alternative tracer for radioisotope in SLN mapping.

Clinical Trial Registration

This clinical trial was registered with the China Clinical Trial Center, registration number ChiCTR2000039542.
IL-21 Is an Accomplice of PD-L1 in the Induction of PD-1-Dependent Treg Generation in Head and Neck Cancer
Authors: Zhao Yi , Zhang Zhiyu , Lei Wenbin , Wei Yi , Ma Renqiang , Wen Yihui , Wei Fanqin , Fan Jun , Xu Yang , Chen Lin , Lyu Kexing , Lin Hanqing , Wen Weiping , Sun Wei
Published: May 2021

Regulatory T cells (Tregs) are immunosuppressive cells involved in antitumor immunity. However, the regulation of Treg generation by inflammation in the tumor microenvironment has not been carefully investigated. Here, we demonstrated that IL-21-polarized inflammation was enriched in the tumor microenvironment in head and neck squamous cell carcinoma (HNSCC) and that IL-21 could promote PD-L1-induced Treg generation in a PD-1-dependent manner. Moreover, generated Tregs showed a greater ability to suppress the proliferation of tumor-associated antigen (TAA)-specific T cells than naturally occurring Tregs. Importantly, an anti-PD-1 antibody could inhibit only Treg expansion induced by clinical tumor explants with high expression of IL-21/PD-L1. In addition, neutralizing IL-21 could enhance the anti-PD-1 antibody-mediated inhibitory effect on Treg expansion. Furthermore, simultaneous high expression of IL-21 and PD-L1 was associated with more Treg infiltrates and predicted reduced overall and disease-free survival in patients with HNSCC. These findings indicate that IL-21 in the tumor microenvironment may promote PD-L1-induced, Treg-mediated immune escape in a PD-1-dependent manner and that an IL-21 neutralization strategy may enhance PD-1 blockade-based antitumor immunotherapy by targeting Treg-mediated immune evasion in patients with high expression of IL-21 and PD-L1.
Surgical and Peri-Operative Considerations for Brain Metastases
Authors: Gupta Saksham , Dawood Hassan , Giantini Larsen Alexandra , Fandino Luis , Knelson Erik H. , Smith Timothy R. , Lee Eudocia Q. , Aizer Ayal , Dunn Ian F. , Bi Wenya Linda
Published: May 2021

Introduction

Brain metastases are the most common brain tumors in adults, whose management remains nuanced. Improved understanding of risk factors for surgical complications and mortality may guide treatment decisions.

Methods

A nationwide, multicenter analysis was conducted with a retrospective cohort. Adult patients in the 2012-2015 American College of Surgeons National Surgical Quality Improvement Project (ACS NSQIP) databases who received a craniotomy for resection of brain metastasis were included.

Results

3500 cases were analyzed, of which 17% were considered frail and 24% were infratentorial. The most common 30-day medical complications were venous thromboembolism (3%, median time-to-event [TTE] 4.5 days), pneumonia (4%, median TTE 6 days), and urinary tract infections (2%, median TTE 5 days). Reoperation and unplanned readmission occurred in 5% and 12% of patients, respectively. Infratentorial approach and frailty were associated with reoperation before discharge (OR 2.0 for both; p=0.01 and p=0.03 respectively), but not after discharge. Infratentorial approaches conferred heightened risk for readmission for hydrocephalus (OR 5.1, p=0.02) and reoperation for cerebrospinal fluid diversion (OR 7.1, p<0.001).Overall 30-day mortality was 4%, with nearly three-quarters occurring after discharge. Pre-frailty and frailty were associated with increased odds for post-discharge mortality (OR 1.7 and 2.7, p<0.05), but not pre-discharge mortality. We developed a model to identify pre-/peri-operative variables associated with death, including frailty, thrombocytopenia, and high American Society of Anesthesiologists score (AUROC 0.75).

Conclusions

Optimization of metrics contributing to patient frailty and heightened surveillance in patients with infratentorial metastases may be considered in the peri-operative period.
miRNA-Based Therapeutics in Breast Cancer: A Systematic Review
Authors: Grimaldi Anna Maria , Salvatore Marco , Incoronato Mariarosaria
Published: May 2021

Background

Breast cancer (BC) is the most common cancer in females and despite advances in treatment, it represents the leading cause of cancer mortality in women worldwide. Conventional therapeutic modalities have significantly improved the management of BC patients, but subtype heterogeneity, drug resistance, and tumor relapse remain the major factors to hamper the effectiveness of therapy for BC. In this scenario, miRNA(miR)-based therapeutics offer a very attractive area of study. However, the use of miR-based therapeutics for BC treatment still represents an underdeveloped topic. Therefore, this systematic review aims at summarizing current knowledge on promising miR-based therapeutics for BC exploring original articles focusing on in vivo experiments.

Methods

The current systematic review was performed according to PRISMA guidelines. PubMed and EMBASE databases were comprehensively explored to perform the article search.

Results

Twenty-one eligible studies were included and analyzed: twelve focused on antitumor miR-based therapeutics and nine on metastatic miR-based therapeutics. We found 18 different miRs tested as potential therapeutic molecules in animal model experiments. About 90% of the selected studies evaluate the efficiency and the safety of miRs as therapeutic agents in triple-negative (TN)-BC mouse models. Among all founded miR-based therapeutics, miR-21 emerged to be the most investigated and proposed as a potential antitumoral molecule for TNBC treatment. Besides, miR-34a and miR-205a appeared to be successful antitumoral and antimetastatic molecules.

Conclusions

Our analysis provides a snapshot of the current scenario regarding the miRs as therapeutic molecules in BC. Nevertheless, despite many efforts, none of the selected studies goes beyond preclinical studies, and their translatability in the clinical practice seems quite premature.
The Complexity of the Tumor Microenvironment and Its Role in Acute Lymphoblastic Leukemia: Implications for Therapies
Authors: Simioni Carolina , Conti Ilaria , Varano Gabriele , Brenna Cinzia , Costanzi Eva , Neri Luca M.
Published: May 2021

The microenvironment that surrounds a tumor, in addition to the tumor itself, plays an important role in the onset of resistance to molecularly targeted therapies. Cancer cells and their microenvironment interact closely between them by means of a molecular communication that mutually influences their biological characteristics and behavior. Leukemia cells regulate the recruitment, activation and program of the cells of the surrounding microenvironment, including those of the immune system. Studies on the interactions between the bone marrow (BM) microenvironment and Acute Lymphoblastic Leukemia (ALL) cells have opened a scenario of potential therapeutic targets which include cytokines and their receptors, signal transduction networks, and hypoxia-related proteins. Hypoxia also enhances the formation of new blood vessels, and several studies show how angiogenesis could have a key role in the pathogenesis of ALL. Knowledge of the molecular mechanisms underlying tumor-microenvironment communication and angiogenesis could contribute to the early diagnosis of leukemia and to personalized molecular therapies. This article is part of a Special Issue entitled: Innovative Multi-Disciplinary Approaches for Precision Studies in Leukemia edited by Sandra Marmiroli (University of Modena and Reggio Emilia, Modena, Italy) and Xu Huang (University of Glasgow, Glasgow, United Kingdom).
Repurposing Kinase Inhibitor Bay 11-7085 to Combat Staphylococcus aureus and Candida albicans Biofilms
Authors: Escobar Iliana E. , Possamai Rossatto Fernanda Cristina , Kim Soo Min , Kang Min Hee , Kim Wooseong , Mylonakis Eleftherios
Published: May 2021

Staphylococcus aureus and Candida spp. are commonly linked with topical biofilm-associated infections such as those found on chronic wounds. These biofilms are notoriously difficult to treat, highlighting the grave need to discover and study new broad-spectrum agents to combat associated infections. Here we report that the kinase inhibitor Bay 11-7085 exhibited bactericidal activity against multidrug-resistant S. aureus with a minimum inhibitory concentration (MIC) of 4 μg/ml. In addition, S. aureus strain MW2 did not acquire resistance to antibiotic pressure. Furthermore, Bay 11-7085 exhibited potency against Candida albicans and the emerging pathogen Candida auris with a MIC of 0.5–1 μg/ml. Bay 11-7085 partially inhibited and eradicated biofilm formation of various pathogens, such as VRSA (vancomycin-resistant S. aureus), as well as antifungal-resistant Candida spp. isolates. Notably, Bay 11-7085 partially inhibited initial cell attachment and formation of a VRSA-C. albicans polymicrobial biofilm in vitro. In contrast to C. albicans, inhibition of VRSA biofilm was linked to initial cell attachment independent of its bactericidal activity. Finally, Bay 11-7085 was effective in vivo at increasing the lifespan of C. elegans during an S. aureus and a C. albicans infection. Our work proposes kinase inhibitor Bay 11-7085 as a potential compound capable of combating biofilms associated with primary multidrug-resistant bacteria and yeast pathogens associated with wound infections.
What Is the Best Discipline to Predict Overall Triathlon Performance? An Analysis of Sprint, Olympic, Ironman® 70.3, and Ironman® 140.6
Authors: Sousa Caio Victor , Aguiar Samuel , Olher Rafael Reis , Cunha Rafael , Nikolaidis Pantelis Theodoros , Villiger Elias , Rosemann Thomas , Knechtle Beat
Published: May 2021

Objective: To analyze the proportion of dedication in each triathlon discipline (swimming, cycling, and running) and the importance of each separate discipline to predict overall performance of elite triathletes across different triathlon distances.

Methods: Data from 2015 to 2020 (n = 16,667) from official races and athletes in Sprint, Olympic distance, IM 70.3 (Half-Ironman distance), and IM 140.6 (Full-Ironman distance) competitions were included. The proportion of each discipline was calculated individually and compared using general linear models by event distance, sex, and performance level. Automatic linear regression models were applied for each distance considering overall performance as the dependent variable.

Results: A within-distance analysis showed that the best predictor for Sprint is cycling, for Olympic is swimming, for IM 70.3 is cycling, and for IM 140.6 is running. A between-distance analysis revealed that swimming is a better predictor in Olympic distance than in other triathlon distances. Cycling is a poor predictor for overall performance in IM 140.6, and the importance of running to predict overall performance is the highest in IM 140.6 and diminishes with decreasing race distance.

Conclusion: Each discipline represents a different relative portion and importance to predict overall performance depending on the triathlon distance. Swimming is the most important predictor discipline in Sprint- and Olympic-distance triathlon, cycling in IM 70.3, and running in IM 140.6.
Transcriptome Analysis of Tetraploid and Octoploid Common Reed (Phragmites australis)
Authors: Wang Cui , Wang Tong , Yin Meiqi , Eller Franziska , Liu Lele , Brix Hans , Guo Weihua
Published: May 2021

Polyploidization in plants is thought to have occurred as coping mechanism with environmental stresses. Polyploidization-driven adaptation is often achieved through interplay of gene networks involved in differentially expressed genes, which triggers the plant to evolve special phenotypic traits for survival.Phragmites australisis a cosmopolitan species with highly variable phenotypic traits and high adaptation capacity to various habitats. The species’ ploidy level varies from 3x to 12x, thus it is an ideal organism to investigate the molecular evolution of polyploidy and gene regulation mediated by different numbers of chromosome copies. In this study, we used high-throughput RNAseq data as a tool, to analyze the gene expression profiles in tetraploid and octoploidP. australis. The estimated divergence time between tetraploid and octoploidP. australiswas dated to the border between Pliocene and Pleistocene. This study identified 439 up- and 956 down-regulated transcripts in tetraploids compared to octoploids. Gene ontology and pathway analysis revealed that tetraploids tended to express genes responsible for reproduction and seed germination to complete the reproduction cycle early, and expressed genes related to defense against UV-B light and fungi, whereas octoploids expressed mainly genes related to thermotolerance. Most differentially expressed genes were enriched in chaperones, folding catalysts and protein processing in endoplasmic reticulum pathways. Multiple biased isoform usage of the same gene was detected in differentially expressed genes, and the ones upregulated in octoploids were related to reduced DNA methylation. Our study provides new insights into the role of polyploidization on environmental responses and potential stress tolerance in grass species.
The Emerging Role of Interdisciplinarity in Clinical Psychoanalysis
Authors: Steinmair Dagmar , Löffler-Stastka Henriette
Published: May 2021

Given the tight interconnections proposed between brain and psyche, psychoanalysis was conceptualized as an interdisciplinary theory right from the beginning. The diversification of knowledge performed by different science and technology fields, concerned with the same matter (explaining mind and brain and connecting them), makes this interdisciplinarity even more visible and evident. This challenges the integrative potential lying in psychoanalytic meta-theory.
“Come on! He Has Never Cooked in His Life!” New Alternative Masculinities Putting Everything in Its Place
Authors: Valls-Carol Rosa , Madrid-Pérez Antonio , Merrill Barbara , Legorburo-Torres Guillermo
Published: May 2021

Communicative acts of some women are perpetuating the dominance that DTM (Dominant Traditional Masculinities) have over both women and OTM (Oppressed Traditional Masculinities). Some women use language in a disdainful manner to reprimand oppressed men’s behavior in daily life situations, the same behavior that such women would not reproach to DTM. But NAM (New Alternative Masculinities) are reacting to this. This article analyzes the communicative acts employed in all these situations, both those produced by women and DTM, as well as NAM’s communicative acts in response to those offenses. Data was collected using communicative daily life stories of give women and three men with diverse profiles and different levels of participation in women’s and men’s movements. Findings highlight, from the transformative dimension of the communicative methodology, that the use of language of desire in NAM’s reactions is effective not only to make justice with men who have never executed violence on women, but also to undermine the attractiveness of both DTM’s behavior and the comments of some women on such behavior. These findings complement previous research on preventive socialization of gender violence by broadening scientific knowledge on NAM’s communicative acts that prevent and eradicate gender-based violence. Further research ought to broaden the evidence of how some women who defend feminist values sometimes do not support and even tease or reprimand men who practice these values; moreover, an important line could analyze the way people talk about men with NAM attitudes to hold back reprimands in comparison to how people talk about men who follow a DTM model.
Augmenting Clinical Interventions in Psychiatric Disorders: Systematic Review and Update on Nutrition
Authors: Offor Samuel J. , Orish Chinna N. , Frazzoli Chiara , Orisakwe Orish E.
Published: May 2021

There is a strong relationship between a healthy diet and mental well-being. Several foods and food compounds are known to modulate biomarkers and molecular mechanisms involved in the aetiogenesis of several mental disorders, and this can be useful in containing the disease progression, including its prophylaxis. This is an updated systematic review of the literature to justify the inclusion and recognition of nutrition in the management of psychiatric illnesses. Such foods and their compounds include dietary flavanols from fruits and vegetables, notable antioxidant and anti-inflammatory agents, probiotics (fermented foods) known to protect good gut bacteria, foods rich in polyunsaturated fatty acids (e.g., Omega-3), and avoiding diets high in saturated fats and refined sugars among others. While the exact mechanism(s) of mitigation of many nutritional interventions are yet to be fully understood, the evidence-based approach warrants the inclusion and co-recognition of nutrition in the management of psychiatric illnesses. For the greater public health benefit, there is a need for policy advocacy aimed at bridging the knowledge gap and encouraging the integration of nutritional intervention with contemporary therapies in clinical settings, as deficiencies of certain nutrients make therapy difficult even with appropriate medication.
Toward Optimal Management of Behavioral and Psychological Symptoms of Dementia: Insights From a COVID-19 Pandemic Experience
Authors: Debas Karen , Beauchamp Joanny , Ouellet Christine
Published: May 2021

The first wave of SARS-CoV-2 has deeply affected long term care facilities in the province of Quebec. In response, governmental officials took protective measures, such as suspending visits and activities and even requiring residents to self-isolate to their room. Consequently, residents with major cognitive impairments were cut from their routine as well as from significant social interactions, support, and stimulation essential to their well-being. This isolation negatively affected many residents. For some of them, the loss of bearings resulted in newly or deteriorated behavioral and psychological symptoms of dementia (BPSD). These residents were then more at risk of contracting the virus or contaminating others. To face this challenge, hotels in the Greater Montreal area were transformed into temporary care facilities. As members of a multidisciplinary team specialized in the management of BPSD, we were asked to support the redeployed staff who had little experience in this domain. In this paper, we present the innovative tools implemented in this uncommon work setting. We also discuss factors identified as facilitating the care and treatment of people with BPSD. This experience leads us to propose avenues toward better BPSD management.
Validity of Short-Term Assessment of Risk and Treatability in the Japanese Forensic Probation Service
Authors: Kikuchi Akiko , Soshi Takahiro , Kono Toshiaki , Koyama Mayuko , Fujii Chiyo
Published: May 2021

This study aimed to evaluate the predictive validity and reliability of the Short-Term Assessment of Risk and Treatability (START) in the context of the Japanese forensic probation service. START is a structured professional judgement guide for risk domains concerning negative behaviors such as violence, self-harm, suicide, substance abuse, unauthorized leave, victimization, and self-neglect. In this study, rehabilitation coordinators evaluated community-dwelling patients who were treated under the Medical Treatment and Supervision Act at baseline and followed-up for 6 months. The results revealed that START vulnerability scores significantly predicted self-harm, suicide, physical aggression, substance abuse, and self-neglect. START strength scores predicted physical violence and unauthorized leave. Specific risk estimates predicted physical violence and self-neglect. Risk judgement for future substance use may require adjustments for cultural differences, because of the lower prevalence in Japan. These results suggest that START offers a feasible and valid tool that allows clinicians to plan treatment and promote recovery of forensic patients in Japan.
Functional Variants Surrounding Endothelin 2 Are Associated With Mycobacterium avium Subspecies paratuberculosis Infection
Authors: Kiser Jennifer N. , Wang Zeping , Zanella Ricardo , Scraggs Erik , Neupane Mahesh , Cantrell Bonnie , Van Tassell Curtis P. , White Stephen N. , Taylor Jeremy F. , Neibergs Holly L.
Published: May 2021

Bovine paratuberculosis, caused by Mycobacterium avium subspecies paratuberculosis (MAP), continues to impact the dairy industry through increased morbidity, mortality, and lost production. Although genome-wide association analyses (GWAAs) have identified loci associated with susceptibility to MAP, limited progress has been made in identifying mutations that cause disease susceptibility. A 235-kb region on Bos taurus chromosome 3 (BTA3), containing a 70-kb haplotype block surrounding endothelin 2 (EDN2), has previously been associated with the risk of MAP infection. EDN2 is highly expressed in the gut and is involved in intracellular calcium signaling and a wide array of biological processes. The objective of this study was to identify putative causal mutations for disease susceptibility in the region surrounding EDN2 in Holstein and Jersey cattle. Using sequence data from 10 Holstein and 10 Jersey cattle, common variants within the 70-kb region containing EDN2 were identified. A custom SNP genotyping array fine-mapped the region using 221 Holstein and 51 Jersey cattle and identified 17 putative causal variants (P < 0.01) located in the 5′ region of EDN2 and a SNP in the 3′ UTR (P = 0.00009) associated with MAP infection. MicroRNA interference assays, mRNA stability assays, and electrophoretic mobility shift assays were performed to determine if allelic changes at each SNP resulted in differences in EDN2 stability or expression. Two SNPs [rs109651404 (G/A) and rs110287192 (G/T)] located within the promoter region of EDN2 displayed differential binding affinity for transcription factors in binding sequences harboring the alternate SNP alleles. The luciferase reporter assay revealed that the transcriptional activity of the EDN2 promoter was increased (P < 0.05) with the A allele for rs109651404 and the G allele for rs110287192. These results suggest that the variants rs109651404 and rs110287192 are mutations that alter transcription and thus may alter susceptibility to MAP infection in Holstein and Jersey cattle.
Summer Diet of Horses (Equus ferus caballus Linn.), Guanacos (Lama guanicoe Müller), and European Brown Hares (Lepus europaeus Pallas) in the High Andean Range of the Coquimbo Region, Chile
Authors: Castellaro Giorgio , Orellana Carla Loreto , Escanilla Juan Pablo
Published: May 2021

For an adequate management of natural grasslands, the knowledge and understanding of the dietary habits of herbivores and their trophic interactions are fundamental. During two summer seasons, in a mountain range of a sector of the Coquimbo Region, Chile, the botanical composition, diversity, and similarity of the diets of horses, European brown hares, and guanacos were studied, as was the selectivity of the main grassland plant species, using feces microhistology. The contribution of hydromorphic grasses was similar in the diets of guanacos (35.90 ± 7.27%) and horses (32.25 ± 4.50%), differing from that found in hares (16.32 ± 5.32%). Dryland grassland grasses contributed similarly to the diets of horses (13.21 ± 3.22%), guanacos (22.53 ± 5.21%) and hares (18.35 ± 3.81%), as well as graminoids, which averaged 47.79 ± 6.66%, 35.63 ± 10.76% and 38.94 ± 7.88%, in diets of horses, guanacos, and hares, respectively, without significant differences. The contribution of herbaceous dicotyledons was only important in hares (23.76 ± 3.76%), while that of shrubs was low (<3%) and similar among the three herbivores. Dietary diversity was similar among the three herbivore species (73–79%), with a higher degree of dietary overlap between horses and guanacos (55.7%), which was higher than that obtained between hares and guanacos (50%) and between horses and hares (48%), for which there would be a potential trophic competition between them. The most abundant species of dryland and wet grasslands generally fulfill a functional role of subsistence and a nutritional role of maintenance; however, for the three herbivores studied, a different selective behavior was evidenced, according to their physiological differences, with the selection process little affected by the relative abundance of these species in the grasslands. Due to the above, herbivores resort to the selection of certain species that, despite being not very abundant in grasslands, play an important nutritional and functional role, improving the quality of their diets.
Differences in Fitness and Academic Attainment between Obese, and Non Obese School-Age Adolescent Handball Players: An Explorative, Cross-Sectional Study
Authors: Hermassi Souhail , Hayes Lawrence D. , Schwesig René
Published: May 2021

This study investigated differences in physical fitness and academic attainment in obese and non-obese adolescent handball players. A total of 31 males (age: 15.5 ± 1.2 years; body mass: 77.8 ± 17.7 kg; height: 1.71 ± 0.10 m; body mass index (BMI): 26.8 ± 6.9 kg/m2; body fat: 26.4 ± 6.34%) from the Qatar handball first division participated and were divided into two body fat percentage (%BF) groups (i.e., obese or non-obese). Anthropometrics (height, mass, BMI, and %BF) and physical performance testing ability (T-half test for change-of-direction (COD); squat jump (SJ), countermovement jump (CMJ), and 10 and 15 m sprints; medicine ball throw (MBT), and aerobic capacity (Yo-Yo Intermittent Recovery Test level 1 (Yo-Yo IR1)) were determined. Academic attainment was determined through grade point averages (GPA). Non-obese participants had superior performances in mathematics (p < 0.001) and science (p = 0.013), agility T-half test (p = 0.001), CMJ (p < 0.001), and 15 m sprint (p = 0.019). Correlations were found between T-half test and mathematics (r = 0.500) and science (r = 0.484). To conclude, obese school-age handball players have poorer fitness and academic performances than normal body weight adolescents.
Effect of Incense Ash on the Engineering Properties of Cement-Based Composite Material
Authors: Kuo Wen-Ten , Juang Chuen-Ul , Chen Tzu-Yi
Published: May 2021

This study investigated the effect of substituting cement with incense ash on material properties and hydration of cement-based composite materials. Test specimens with incense ash replacing up to 30% (volume) of cement were produced and cured at 25 °C and 75 °C for up to 120 days. The researchers of this study observed the effect on setting time, heat conduction, compressive strength, and water absorption, and conducted a microanalysis to observe the structural change of hydration products. The results indicated that by substituting cement with incense ash, the hydration reaction was hastened, and the setting time shortened. When 5% of cement was replaced, incense ash had the effect of filling the voids between particles. At a higher curing temperature, the frequency of particle collisions increased, expediting the hydration reaction and improving the strength of the specimens. At a longer curing time, voids in the specimens were gradually filled by colloids, and pozzolanic reaction caused the amount of Ca(OH)2 to diminish and C–S–H gel to increase, which in turn improved the structural cohesion, reduced the water absorption, and increased the strength and thermal conductivity of the specimens.
An Updated Method for Stability Analysis of Milling Process with Multiple and Distributed Time Delays and Its Application
Authors: Jin Gang , Li Wenshuo , Han Jianxin , Li Zhanjie , Hu Gaofeng , Sun Guangxing
Published: May 2021

Predicting and avoiding the onset of milling chatter are desirable to reduce its harm to machine tools, workpieces, and cutters. This paper presents an updated method to complete the stability prediction for the milling process with multiple and distributed time delays. After the dynamic of the combination milling process with variable helix cutter (VHC) and variable spindle speed (VSS) is modeled as linear delay differential equations with multiple and distributed time delays, the presented method is applied to carrying out its stability prediction for the first time. By comparing with the existing researches and time-domain simulations, the effectiveness of the presented method has been validated. The influence and feasibility of the combination process on chatter suppression are explored and investigated for the associated one- and two-degree-of-freedom systems. Results show that the application of the combination process can realize a further suppression of milling chatter in practice. It can result in nearly 2-fold as high as the minimum depth of cut for the traditional milling or VSS milling and about 1.3-fold for VHC milling for some special domain, and can respectively lead to the average increase of stable area by 30.4%, 23.5%, and 1.5% for the adopted simulations. However, consider the contribution, the combination process is actually one process in which VHC plays an absolutely leading role but VSS plays an auxiliary role, in terms of milling stability.