bluuebunny/crossref_metadata_2025 huggingface dataset:
Possible effects of left pulmonary artery stenting in single ventricle patients on bronchial area, lung volume and lung function
Authors: Callegari Alessia , Geiger Julia , Callaghan Fraser Maurice , Kellenberger Christian , Usemann Jakob , Burkhardt Barbara Elisabeth Ursula , Kretschmar Oliver , Valsangiacomo Büchel Emanuela
Published: January 2024

Background

Left pulmonary artery (LPA) stenting is often required in single ventricle (SV) patients. Due to their close anatomical relationship an LPA stent could potentially compress the left main bronchus (LMB). We assessed the impact of LPA stenting on bronchial size, pulmonary volumes, and lung function in a cohort of SV patients.

Methods

Forty-nine patients underwent cardiovascular magnetic resonance (CMR) and 36 spirometry 11 (8–15) years after Fontan. All patients were free of respiratory symptoms. LPA stents were inserted in 17 (35%) patients at 8.8 (3.4–12.6) years. Area/shape of the main bronchi (n = 46) and lung volumes (n = 47) were calculated from CMR-ZTE images for each lung and transformed in right-to-left (r/l) ratio and indexed for BSA. The effect of early stent insertion (prior to stage III) was analyzed.

Results

Patients with LPA stent had larger r/l ratio for main bronchus area (p < 0.001) and r/l ratio difference for lung volumes was slightly larger in patients with early stenting. A trend toward a deformation of LMB shape in patients with LPA stent and toward a higher prevalence of abnormal spirometry in patients with early stent implantation was observed.

Conclusions

In this cohort of patients, early insertion of LPA stents seems to relate with smaller LMB sizes and a trend toward smaller left lung volume and higher prevalence of impaired lung function. Whether these findings are caused by the stent or, at least to a certain degree, present prior to the implantation needs to be verified.
Identifying new safety risk of human serum albumin: a retrospective study of real-world data
Authors: Lu Hui , Zhang Yuwei , Liu Pengcheng
Published: January 2024

Objective: To mine and analyze the adverse reaction signals of human serum albumin (HSA) using the FDA adverse event reporting system (FAERS) database for the safe clinical use of this drug.

Methods: Data cleaning and analysis of adverse event reports in the FAERS database for a total of 76 quarters from Q1 2004 to Q4 2022 were performed using the reporting odds ratio (ROR), Medicines and Healthcare Products Regulatory Agency (MHRA), and Bayesian confidence propagation neural network (BCPNN). Gender-differentiated signal detection was used to investigate the gender differences in the occurrence of HSA adverse events.

Results: Through a combination of three methods, a total of 535 adverse event reports were identified. These reports involved 1,885 cases of adverse reactions, with respiratory, thoracic, and mediastinal disorders, as well as general disorders and administration site conditions, as the most common. One noteworthy new signal was the occurrence of transfusion-related acute lung injury. Additionally, gender-differentiated signals were present, with females experiencing paraesthesia, hypertension, pulmonary oedema, loss of consciousness, and vomiting.

Conclusion: This study has revealed that HSA poses a risk of causing transfusion-related acute lung injury. It has also been observed that adverse reactions, including paraesthesia, hypertension, pulmonary oedema, loss of consciousness, and vomiting, are more prevalent in females. These findings should be taken into account when using HSA in a clinical setting.
Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources
Authors: Fuller S. Adam , Abernathy Jason W. , Sankappa Nithin Muliya , Beck Benjamin H. , Rawles Steven D. , Green Bartholomew W. , Rosentrater Kurt A. , McEntire Matthew E. , Huskey George , Webster Carl D.
Published: January 2024

White bass (Morone chrysops) are a popular sportfish throughout the southern United States, and one parent of the commercially-successful hybrid striped bass (M. chrysops ♂ x M. saxatilis ♀). Currently, white bass are cultured using diets formulated for other carnivorous fish, such as largemouth bass (Micropterus salmoides) or hybrid striped bass and contain a significant percentage of marine fish meal. Since there are no studies regarding the utilization of alternative proteins in this species, we evaluated the global gene expression of white bass fed diets in which fish meal was partially or totally replaced by various combinations of soybean meal, poultry by-product meal, canola meal, soy protein concentrate, wheat gluten, or a commercial protein blend (Pro-Cision™). Six isonitrogenous (40% protein), isolipidic (11%), and isocaloric (17.1 kJ/g) diets were formulated to meet the known nutrient and energy requirements of largemouth bass and hybrid striped bass using nutrient availability data for most of the dietary ingredients. One of the test diets consisted exclusively of plant protein sources. Juvenile white bass (40.2 g initial weight) were stocked into a flow-through aquaculture system (three tanks/diet; 10 fish/tank) and fed the test diets twice daily to satiation for 60 days. RNA sequencing and bioinformatic analyses revealed significant differentially expressed genes between all test diets when compared to fish meal control. A total of 1,260 differentially expressed genes were identified, with major ontology relating to cell cycle and metabolic processes as well as immune gene functions. This data will be useful as a resource for future refinements to moronid diet formulation, as marine fish meal becomes limiting and plant ingredients are increasingly added as a reliable protein source.
Bridging the gap: unleashing the power of non-core departments through interdepartmental collaboration
Authors: Ren Siyu , Wang Yile
Published: January 2024

Traditional research on firm performance has predominantly emphasized the role of key departments, often underestimating the potential contributions of non-core departments. This study redresses this oversight by investigating the impact of non-core departments on firm performance. Utilizing a comprehensive 20-year dataset from Chinese A-listed firms and employing the endogenous growth model, we scrutinize the influence of non-core departments on enterprise productivity and organizational growth. Our findings underscore that non-core departments significantly enhance firm performance. Furthermore, we observe a negative coefficient of the interaction term, implying the presence of diminishing returns to scale when amalgamating department diversity with firm knowledge. This suggests that while both department diversity and firm knowledge independently contribute positively to firm performance, their conjoined effect does not necessarily induce a proportionally amplified impact. Moreover, we found that factors such as the company’s equity structure, market environment, and the age and education level of executives may moderate the impact of departmental diversity on firm performance. This study enriches the literature by spotlighting the potential of non-core departments in propelling firm success and underlines the imperative for strategies that cultivate interdepartmental collaboration. The implications of these findings propose that firms can leverage the potential of non-core departments for sustainable growth, offering a fresh perspective for future research in organizational development.
Prosocial and externalizing behaviors in children raised by different-and same-gender parent families: new directions in parenting research
Authors: Baiocco Roberto , Favini Ainzara , Pistella Jessica , Carone Nicola , Speranza Anna Maria , Lingiardi Vittorio
Published: January 2024

Introduction

Limited research focused on the association between parenting practices and children’s prosocial and externalizing behaviors comparing same- and different-gender parent families. The present study considered 76 Italian families (73% same-gender and 27% different-gender parent families) with 8-year-old (SD = 2.17; 49% assigned female at birth) children born through assisted reproductive techniques, to explore parenting practices and children’s prosocial and externalizing behaviors.

Method

We ran a Multiple-group-by-couple Structural Equation Model in which we estimated the predictive role of parenting on children’s behaviors, controlling for age, gender, and family socioeconomic status using the Maximum Likelihood estimation.

Results

Results showed that both same- and different-gender parent families reported high levels of parental warmth and very low levels of hostility and rejection; regarding children’s behaviors, both same- and different-gender parent families reported high levels of prosociality and low levels of externalizing behaviors. In addition, same-gender parents reported significantly higher levels of children’s prosociality and parental warmth than different-gender parents. Regarding associations between parenting practices and behaviors, we found a positive association between positive parenting practices and increasing children’s prosocial behaviors and decreasing children’s externalizing behaviors, in both same- and different-gender families, controlling for family background characteristics.

Conclusion

The present study encourages future research to investigate how specific parenting practices can influence behavioral adjustment in children, focusing on same-gender parent families.
Homing wellness: can narrative design transform living spaces for people with dementia into engaging environments enabling communication?
Authors: Minetou Loukia , Chatzopoulos Avraam , Tzerachoglou Anastasios , Priniotakis Georgios , van Hoof Joost , Sfyroera Emmanouela , Georgiadou Zoe , Tyrovola Styliani , Drosos Christos
Published: January 2024

Interactive design is an emerging trend in dementia care environments. This article describes a research project aiming at the design and development of novel spatial objects with narrative attributes that incorporate embedded technology and textiles to support the wellbeing of people living with dementia. In collaboration with people with dementia, this interdisciplinary research project focuses on the question of how innovative spatial objects can be incorporated into dementia long-term care settings, transforming the space into a comforting and playful narrative environment that can enhance self-esteem while also facilitating communication between people living with dementia, family, and staff members. The research methodologies applied are qualitative, including Action Research. Participatory design methods with the experts by experience—the people with dementia—and health professionals have been used to inform the study. Early findings from this research are presented as design solutions comprising a series of spatial object prototypes with embedded technology and textiles. The prototypes were evaluated primarily by researchers, health professionals, academics, and design practitioners in terms of functionality, aesthetics, and their potential to stimulate engagement. The research is ongoing, and the aim is to evaluate the prototypes by using ethnographic and sensory ethnography methods and, consequently, further develop them through co-design workshops with people living with dementia.
Disaster-related deaths with alcohol-related diseases after the Fukushima Daiichi nuclear power plant accident: case series
Authors: Kitazawa Kemmei , Sawano Toyoaki , Uchi Yuna , Kawashima Moe , Yoshimura Hiroki , Murakami Michio , Nonaka Saori , Saito Hiroaki , Sakakibara Mamoru , Yagiuchi Kazuko , Otsuki Mako , Ozaki Akihiko , Yamamoto Chika , Zhao Tianchen , Uchiyama Taiga , Oikawa Tomoyoshi , Niwa Shinichi , Tsubokura Masaharu
Published: January 2024

Introduction

The health of patients with mental disorders, such as alcohol-related diseases, often deteriorates after disasters. However, the causes of death among those with alcohol-related diseases during and after radiation disasters remain unclear.

Methods

To minimize and prevent alcohol-related deaths in future radiation disasters, we analyzed and summarized six cases of alcohol-related deaths in Minamisoma City, a municipality near the Fukushima Daiichi nuclear power plant.

Results

Patients were generally treated for alcohol-related diseases. In one case, the patient was forced to evacuate because of hospital closure, and his condition worsened as he was repeatedly admitted and discharged from the hospital. In another case, the patient’s depression worsened after he returned home because of increased medication and drinking for insomnia and loss of appetite.

Discussion

The overall findings revealed that, in many cases, evacuation caused diseases to deteriorate in the chronic phase, which eventually resulted in death sometime after the disaster. To mitigate loss of life, alcohol-related diseases must be addressed during the chronic phases of future large-scale disasters, including nuclear disasters.
Sex differences in patients with COVID-19 after bariatric surgery: a multicenter cross-sectional study
Authors: Wang Senlin , Jang Qiubai , Wang Han , Yang Yunning , Ruan Min , Yu Juan , Li Xiuying , Luo Dan
Published: January 2024

Objectives

This multicenter, cross-sectional study aimed to investigate whether sex differences persist among patients who have undergone bariatric surgery and tested positive for the coronavirus disease (COVID-19).

Methods

We conducted a multicenter cross-sectional study via an online electronic questionnaire to collect data. Categorical data were presented as absolute and relative frequencies. Data for continuous variables were expressed as mean and standard deviation (SD) or median [interquartile range (IQR)]. We employed ordered logistic regression to assess whether females had higher odds of an increased self-reported duration of the most severe symptom compared to males. Using a modified Poisson regression model with robust standard errors to assess the differences in clinical characteristics among COVID-19 cases.

Results

Statistical analysis revealed significant differences in the prevalence rates of various comorbidities. Among participants who reported their temperature during COVID-19 infection, more than half engaged in vitamin supplementation and regular exercise, while 4.2% remained asymptomatic. The probability of females experiencing a longer duration of severe symptoms increased compared to males [adjusted Odds Ratio (aOR) = 1.92, 95% confidence interval (CI) 1.73–2.12]. In the multivariate mixed-effects Poisson regression analysis, compared to males, females exhibited a lower prevalence rate of asymptomatic infection [adjusted prevalence ratio (aPR 0.40, 95% CI 0.28–0.58), lower prevalence of infection without therapeutic medication use (aPR 0.76, 95% CI 0.70–0.82), and lower prevalence of multiple infections (aPR 0.39, 95% CI 0.20–0.74)].

Conclusion

This cross-sectional study indicates the persistence of sex differences among patients with COVID-19 who have undergone bariatric surgery. Further research is needed to explore the underlying factors contributing to this disparity.
Demand for emergency services during the COVID-19 pandemic and disease burden: a case study in Portugal
Authors: Nunes Alcina , Costa Catarina , Martins João P. , Ferreira Pedro L. , Pimenta Rui
Published: January 2024

Background

The COVID-19 pandemic brought changes in the pattern of care use. A significant increase in the volume of emergencies was expected. However, a significant decrease was observed worldwide.

Methods

An observational, analytical and cross-sectional study of all records of emergency episodes of patients aged 18 years or older admitted to the emergency services of the University of Porto Hospital Centre (2018–2022) were analysed.

Results

During the pandemic, a significant reduction in emergency episode admissions (up to 40% during lockdowns), an increase in pre-emergency services, and discharges from Infectious Diseases and Internal Medicine was observed. The discharges from General Practice and General Practice and Family Medicine were residual.

Conclusion

The lower use and type of use of emergency services during the COVID-19 pandemic had a negative impact on the disease burden. This could be prevented in future pandemics through the development of strategies to promote confidence in the use of health resources and establishing contingency plans for virtual assistance.
Effect of larval handling on black soldier fly life history traits and bioconversion efficiency
Authors: Loiotine Zaira , Gasco Laura , Biasato Ilaria , Resconi Andrea , Bellezza Oddon Sara
Published: January 2024

Introduction

The black soldier fly is considered the most promising insect species for mass production; however, information on the effects of handling, which is unavoidable during experimental trials and rearing practices, is still limited.

Materials and methods

To address this gap, three different manipulation intensities were tested on 100 6-day-old larvae per replica (6 replicates/treatments) fed on Gainesville diet: (1) hard-handled (HH), larvae underwent continuous manipulation until the end of larval stage, (2) soft-handled (SH), larvae were manipulated after the appearance of the first prepupa, (3) no-handled (NH), larvae remained untouched. Every 4 days from the beginning to the end of the larval stage, the manipulations lasted 30 min and occurred under laboratory conditions (20°C). During the sampling operations, at least 30 larvae were randomly extracted, washed, dried, and weight-mimicked. At the end of larval stage, all the boxes remained untouched until the adult fly stage, and the emergency rate and sex ratio were evaluated on dead flies. Data were statistically analyzed using IBM SPSS V20.0.0 software and the considered significance level was p < 0.05.

Results

The larval stage lasted 8.2 days for both HH and SH (p > 0.05). Despite the HH larvae being the most manipulated, no difference was also observed in final weight (HH, 160 mg; SH, 150 mg; p > 0.05) and survival rate (HH, 96.2%; SH, 94.5%; p > 0.05). The manipulation did not influence the bioconversion capacity of the larvae (bioconversion efficiency corrected for the residue: HH, 14.3%; SH, 12.91%; reduction rate: HH, 58.4%; SH, 55.9%; waste reduction index: HH, 7.28%/day; SH, 7.25%/day; p > 0.05). Finally, the development time from larva to fly (about 20.7; p > 0.05), the emergency rate (NH: 92.8%; SH: 89.5%; HH: 82.7%) and sex ratio (~1.2% to male flies) were not affected by the handling (p > 0.05).

Discussion

In conclusion, the handling procedures used in the current study did not influence the life history traits of the black soldier fly. However, further studies are needed to evaluate if different experimental protocols on various scales, the colony strain or other handling procedures may suggest a different scenario or confirm the results.
Cartesian Stiffness Shaping of Compliant Robots—Incremental Learning and Optimization Based on Sequential Quadratic Programming
Authors: Knežević Nikola , Petrović Miloš , Jovanović Kosta
Published: January 2024

Emerging robotic systems with compliant characteristics, incorporating nonrigid links and/or elastic actuators, are opening new applications with advanced safety features, as well as improved performance and energy efficiency in contact tasks. However, the complexity of such systems poses challenges in modeling and control due to their nonlinear nature and model variations over time. To address these challenges, the paper introduces Locally Weighted Projection Regression (LWPR) and its online learning capabilities to keep the model of compliant actuators accurate and enable the model-based controls to be more robust. The approach is experimentally validated in Cartesian position and stiffness control for a 4 DoF planar robot driven by Variable Stiffness Actuators (VSA), whose real-time implementation is supported by the Sequential Least Squares Programming (SLSQP) optimization approach.
A Novel Control Method for Permanent Magnet Synchronous Linear Motor Based on Model Predictive Control and Extended State Observer
Authors: Dong Zhuobo , Sun Zheng , Sun Hao , Wang Wenjun , Mei Xuesong
Published: January 2024

Permanent magnet synchronous linear motor (PMSLM) is widely used to meet the requirement of high dynamic accuracy positioning, such as in machine tools and devices of semiconductor manufacturing. A new 2-DOF control structure is proposed in this paper to improve the dynamic performance of the positioning servo system with PMSLM. Aiming at the position tracking performance, a control algorithm based on the model predictive control (MPC) is developed with position and speed as the feedback state variables. In addition, an extended state observer (ESO) is designed for the rejection of various disturbances, which are not involved in the control model and are regarded as the lumped disturbance to be estimated and compensated by the ESO. The experimental results show that, compared with the commonly used PPI controller (proportional position controller and proportional–integral speed controller), the proposed method enhances the position bandwidth and servo stiffness effectively.
In Vitro Propagation of Commercially Used Krymsk 5® (Prunus fruticosa × Prunus lannesiana) Cherry Rootstock: Impact of Sugar Types and pH Levels
Authors: Tsafouros Athanasios , Roussos Petros A.
Published: January 2024

In the present study, the effects of different types of sugars and cultivation medium pH levels on the micropropagation of Krymsk 5® cherry rootstock were investigated. During the proliferation stage, the effects of four sugars (sucrose, fructose, glucose, and sorbitol) both separately and in two combinations were studied, along with the effects of pre-adjusted pH (4.5, 5.0, 5.2, 5.5, 5.8, 6.0, 6.2, or 6.5) on shoot proliferation parameters, growth medium’s post-autoclaving and post-cultivation pH, and their relations. Similarly, during the rooting stage, the effects of four sugars (sucrose, glucose, fructose, or sorbitol) at three concentrations (1% w/v, 2% w/v, or 3% w/v) without any auxin inclusion were studied as well as the effects of two sugars (sucrose or fructose) at six pre-adjusted pH levels (4.8, 5.2, 5.8, 6.2, or 6.5), also in the absence of auxin, on rooting parameters. Explants cultivated in fructose-supplemented growth mediums exhibited superior proliferation performance, characterized by the highest values of shoots per explant, shoot length, and nodes per explant. Generally, the medium’s pH decreased after autoclaving, and proliferation performance was favored by low pH values (either pre-adjusted or post-autoclaving). As far as rooting is concerned, fructose inclusion induced a higher rooting percentage (88%) compared to sucrose. The highest rooting was obtained in fructose-supplemented rooting mediums at concentrations of 2% or 3% w/v (95% rooting in both cases), in the absence of auxins. Post-autoclaving pH in fructose-supplemented rooting mediums was lower and buffered in low pH levels than in sucrose-supplemented ones, and the rooting of explants in all pH combinations with fructose exceeded 75%. In addition, rooting was negatively correlated with the post-autoclaving pH. These findings underscore the significance of both the sugar type and the post-autoclaving pH of the medium in both proliferation and rooting stages, highlighting their possible physiological, biochemical, or hormonal effects. Additionally, rooting without the use of auxin, but with the correct choice of sugar, emerges with both financial and environmental benefits, whereas fructose could be potentially used as a buffering agent.
Research on Real-Time Detection of Maize Seedling Navigation Line Based on Improved YOLOv5s Lightweighting Technology
Authors: Gong Hailiang , Wang Xi , Zhuang Weidong
Published: January 2024

This study focuses on real-time detection of maize crop rows using deep learning technology to meet the needs of autonomous navigation for weed removal during the maize seedling stage. Crop row recognition is affected by natural factors such as soil exposure, soil straw residue, mutual shading of plant leaves, and light conditions. To address this issue, the YOLOv5s network model is improved by replacing the backbone network with the improved MobileNetv3, establishing a combination network model YOLOv5-M3 and using the convolutional block attention module (CBAM) to enhance detection accuracy. Distance-IoU Non-Maximum Suppression (DIoU-NMS) is used to improve the identification degree of the occluded targets, and knowledge distillation is used to increase the recall rate and accuracy of the model. The improved YOLOv5s target detection model is applied to the recognition and positioning of maize seedlings, and the optimal target position for weeding is obtained by max-min optimization. Experimental results show that the YOLOv5-M3 network model achieves 92.2% mean average precision (mAP) for crop targets and the recognition speed is 39 frames per second (FPS). This method has the advantages of high detection accuracy, fast speed, and is light weight and has strong adaptability and anti-interference ability. It determines the relative position of maize seedlings and the weeding machine in real time, avoiding squeezing or damaging the seedlings.
Improved YOLOv5 Network for Detection of Peach Blossom Quantity
Authors: Sun Li , Yao Jingfa , Cao Hongbo , Chen Haijiang , Teng Guifa
Published: January 2024

In agricultural production, rapid and accurate detection of peach blossom bloom plays a crucial role in yield prediction, and is the foundation for automatic thinning. The currently available manual operation-based detection and counting methods are extremely time-consuming and labor-intensive, and are prone to human error. In response to the above issues, this paper proposes a natural environment peach blossom detection model based on the YOLOv5 model. First, a cascaded network is used to add an output layer specifically for small target detection on the basis of the original three output layers. Second, a combined context extraction module (CAM) and feature refinement module (FSM) are added. Finally, the network clusters and statistically analyzes the range of multi-scale channel elements using the K-means++ algorithm, obtaining candidate box sizes that are suitable for the dataset. A novel bounding box regression loss function (SIoU) is used to fuse the directional information between the real box and the predicted box to improve detection accuracy. The experimental results show that, compared with the original YOLOv5s model, our model has correspondingly improved AP values for identifying three different peach blossom shapes, namely, bud, flower, and falling flower, by 7.8%, 10.1%, and 3.4%, respectively, while the final mAP value for peach blossom recognition increases by 7.1%. Good results are achieved in the detection of peach blossom flowering volume. The proposed model provides an effective method for obtaining more intuitive and accurate data sources during the process of peach yield prediction, and lays a theoretical foundation for the development of thinning robots.
Numerical Simulation of an Online Cotton Lint Sampling Device Using Coupled CFD–DEM Analysis
Authors: Wang Peiyu , Wang Huting , Zhang Ruoyu , Hu Rong , Hao Beibei , Huang Jie
Published: January 2024

Cotton processing is the process of converting harvested seed cotton into lint by cleaning, ginning, and cleaning the lint. The real-time acquisition of lint parameters during processing is critical in improving cotton processing quality and efficiency. The existing online inspection system cannot realize quantitative sampling detection, resulting in large fluctuations in the detection of moisture rate, and the impurity content of lint can only be measured according to the number of impurity grains and the percentage of impurity areas. This research developed a quantitative sampling device for cotton lint processing that can collect the right number of cotton samples and obtain the weight of the samples, laying the foundation for the accurate detection of cotton lint dampness and impurity rates. This research aimed to develop an online quantitative sampling device with a sampling plate as its core. The quantitative sampling procedure, consisting of a gas–solid two-phase flow in a cotton pipeline, was numerically simulated and experimentally analyzed using computational fluid dynamics (CFD) and the discrete element method (DEM). According to the coupling results, the maximum pressure differential between the top and bottom regions of the sampling plate when conveying was 1024.45 Pa. This pressure is adequate to allow for cotton samples to accumulate on the sampling plate. Simultaneously, the steady conveying speed of lint is 59.31% of the unloaded conveying wind speed, providing a theoretical foundation for the sampling time of the quantitative sample device in the processing chain. The results from testing the prototype indicate that the quantitative sampling device in the cotton flow can effectively perform the quantitative sampling of cotton lint under uniform conditions, with a sampling pass rate of 84%.
Chemical Profile of Cell Cultures of Kalanchoë gastonis-bonnieri Transformed by Agrobacterium rhizogenes
Authors: Barrera Núñez María Guadalupe , Bueno Mónica , Molina-Montiel Miguel Ángel , Reyes-Vaquero Lorena , Ibáñez Elena , Del Villar-Martínez Alma Angélica
Published: January 2024

Kalanchoë gastonis-bonnieri Raym.-Hamet & Perrier is a plant used for medicinal purposes in the treatment of several ailments. The aim of this study was to analyze the chemical profile of extracts from K. gastonis-bonnieri embryogenic calli, generated from genetically transformed roots by Agrobacterium rhizogenes. Putative transformants were verified by PCR. Hydroalcoholic extracts were obtained and the chemical profile was analyzed by LC-ESI-MS/MS. Root formation was obtained from 80% of infected seedlings. Fifteen root lines were isolated, and two lines showed prominent longitudinal growth and profuse branching in the B5 semi-solid medium. In all lines, the formation of nodules and later embryogenic callus was observed. Putative transgenic root lines were cultivated in free-plant growth regulators B5 medium. In the two selected lines, the PCR amplification of rolA, rolB, rolC, rolD, and aux1 genes was detected. The extract of embryogenic calli showed 60 chemical compounds tentatively identified, such as ferulic acid, quinic acid, neobaisoflavone, and malic acid, among others, and the chemical profile was different in comparison to wild-type extracts. This is the first study reporting the analysis of the chemical profile of hairy root extracts derived from Kalanchoë gastonis-bonnieri. This work displays the great potential for obtaining chemical compounds of pharmacological importance from hairy roots and facilitates the identification of new useful drugs against human chronic-degenerative diseases.
1H-NMR-Based Chemometric Analysis of Echinacea Species to Predict Effectors of Myeloid Progenitor Stimulation
Authors: Nagumalli Suresh K. , Salley Joshua T. , Carstens Jeffrey D.
Published: January 2024

Echinacea, a herbaceous, perennial flowering plant from the Compositae (Asteraceae) family, exhibits stimulating effects on myeloid progenitors (CFU-GMs) in rat bone marrow, as demonstrated in our previous study using a 75% (v v−1) ethanol extract of aerial parts. Expanding on this work, we have investigated eleven different Echinacea samples that belong to three species for their myeloid progenitor-stimulating activity. Simultaneously, we employed 1H-NMR spectroscopy (400 MHz, 0.02–10.02 ppm) and chemometric analysis to predict constituents responsible for activity. Female Sprague–Dawley rats received oral doses of ethanol extracts (0–200 mg extract dry weight kg−1 body weight) of Echinacea for seven days. Bone marrow cells were then cultured with CFU-GM growth factors. Extracts showing a statistically significant (p < 0.05) increase in CFU-GM, compared to the control, were considered active. Significant CFU-GM increases were observed in rats treated with seven Echinacea samples, ranging from 39% to 91% higher than the control, while four samples were inactive. All five Echinacea purpurea samples showed myeloid progenitor-stimulating activity, while one sample each of Echinacea angustifolia and Echinacea pallida also exhibited the same activity. By applying orthogonal partial least squares discriminant analysis (OPLS-DA) to the 1H-NMR spectra, we identified specific spectral bins (0.70–1.98 ppm aliphatic and 6.38–7.76 ppm aromatic) correlating with myelopoiesis stimulation. These findings highlight the potential of chemometric analysis using 1H-NMR spectroscopy to infer the chemical classes that could be responsible for the bioactive properties of complex herbal mixtures, like Echinacea.
Salmonellosis: An Overview of Epidemiology, Pathogenesis, and Innovative Approaches to Mitigate the Antimicrobial Resistant Infections
Authors: Lamichhane Bibek , Mawad Asmaa M. M. , Saleh Mohamed , Kelley William G. , Harrington Patrick J. , Lovestad Cayenne W. , Amezcua Jessica , Sarhan Mohamed M. , El Zowalaty Mohamed E. , Ramadan Hazem , Morgan Melissa , Helmy Yosra A.
Published: January 2024

Salmonella is a major foodborne pathogen and a leading cause of gastroenteritis in humans and animals. Salmonella is highly pathogenic and encompasses more than 2600 characterized serovars. The transmission of Salmonella to humans occurs through the farm-to-fork continuum and is commonly linked to the consumption of animal-derived food products. Among these sources, poultry and poultry products are primary contributors, followed by beef, pork, fish, and non-animal-derived food such as fruits and vegetables. While antibiotics constitute the primary treatment for salmonellosis, the emergence of antibiotic resistance and the rise of multidrug-resistant (MDR) Salmonella strains have highlighted the urgency of developing antibiotic alternatives. Effective infection management necessitates a comprehensive understanding of the pathogen’s epidemiology and transmission dynamics. Therefore, this comprehensive review focuses on the epidemiology, sources of infection, risk factors, transmission dynamics, and the host range of Salmonella serotypes. This review also investigates the disease characteristics observed in both humans and animals, antibiotic resistance, pathogenesis, and potential strategies for treatment and control of salmonellosis, emphasizing the most recent antibiotic-alternative approaches for infection control.
Innovative Techniques for Infection Control and Surveillance in Hospital Settings and Long-Term Care Facilities: A Scoping Review
Authors: Arzilli Guglielmo , De Vita Erica , Pasquale Milena , Carloni Luca Marcello , Pellegrini Marzia , Di Giacomo Martina , Esposito Enrica , Porretta Andrea Davide , Rizzo Caterina
Published: January 2024

Healthcare-associated infections (HAIs) pose significant challenges in healthcare systems, with preventable surveillance playing a crucial role. Traditional surveillance, although effective, is resource-intensive. The development of new technologies, such as artificial intelligence (AI), can support traditional surveillance in analysing an increasing amount of health data or meeting patient needs. We conducted a scoping review, following the PRISMA-ScR guideline, searching for studies of new digital technologies applied to the surveillance, control, and prevention of HAIs in hospitals and LTCFs published from 2018 to 4 November 2023. The literature search yielded 1292 articles. After title/abstract screening and full-text screening, 43 articles were included. The mean study duration was 43.7 months. Surgical site infections (SSIs) were the most-investigated HAI and machine learning was the most-applied technology. Three main themes emerged from the thematic analysis: patient empowerment, workload reduction and cost reduction, and improved sensitivity and personalization. Comparative analysis between new technologies and traditional methods showed different population types, with machine learning methods examining larger populations for AI algorithm training. While digital tools show promise in HAI surveillance, especially for SSIs, challenges persist in resource distribution and interdisciplinary integration in healthcare settings, highlighting the need for ongoing development and implementation strategies.
Extracellular Vesicles from a Biofilm of a Clinical Isolate of Candida albicans Negatively Impact on Klebsiella pneumoniae Adherence and Biofilm Formation
Authors: Imparato Marianna , Maione Angela , Buonanno Annalisa , Gesuele Renato , Gallucci Noemi , Corsaro Maria Michela , Paduano Luigi , Casillo Angela , Guida Marco , Galdiero Emilia , de Alteriis Elisabetta
Published: January 2024

The opportunistic human fungal pathogen Candida albicans produces and releases into the surrounding medium extracellular vesicles (EVs), which are involved in some processes as communication between fungal cells and host–pathogen interactions during infection. Here, we have conducted the isolation of EVs produced by a clinical isolate of C. albicans during biofilm formation and proved their effect towards the ability of the Gram-negative bacterial pathogen Klebsiella pneumoniae to adhere to HaCaT cells and form a biofilm in vitro. The results represent the first evidence of an antagonistic action of fungal EVs against bacteria.
In Vitro Antimicrobial Activity of Five Newly Approved Antibiotics against Carbapenemase-Producing Enterobacteria—A Pilot Study in Bulgaria
Authors: Markovska Rumyana , Stankova Petya , Stoeva Temenuga , Keuleyan Emma , Mihova Kalina , Boyanova Lyudmila
Published: January 2024

To solve the problem with pan-drug resistant and extensively drug-resistant Gram-negative microbes, newly approved drugs such as ceftazidime/avibactam, cefiderocol, plazomicin, meropenem/vaborbactam, and eravacycline have been introduced in practice. The aim of the present study was to collect carbapenemase-producing clinical Enterobacterales isolates, to characterize their carbapenemase genes and clonal relatedness, and to detect their susceptibility to commonly used antimicrobials and the above-mentioned newly approved antibiotics. Sixty-four carbapenemase producers were collected in a period of one year from four Bulgarian hospitals, mainly including Klebsiella pneumoniae (89% of the isolates) and also single Proteus mirabilis, Providencia stuartii and Citrobacter freundii isolates. The main genotype was blaNDM-1 (in 61%), followed by blaKPC-2 (23%), blaVIM-1 (7.8%) and blaOXA-48 (7.8%). Many isolates showed the presence of ESBL (blaCTX-M-15/-3 in 76.6%) and AmpC (blaCMY-4 in 37.5% or blaCMY-99 in 7.8% of isolates). The most common MLST type was K. pneumoniae ST11 (57.8%), followed by ST340 (12.5%), ST258 (6.3%) and ST101 (6.3%). The isolates were highly resistant to standard-group antibiotics, except they were susceptible to tigecycline (83.1%), colistin (79.7%), fosfomycin (32.8%), and aminoglycosides (20.3–35.9%). Among the newly approved compounds, plazomicin (90.6%) and eravacycline (76.3%) showed the best activity. Susceptibility to ceftazidime/avibactam and meropenem/vaborbactam was 34.4% and 27.6%, respectively. For cefiderocol, a large discrepancy was observed between the percentages of susceptible isolates according to EUCAST susceptibility breakpoints (37.5%) and those of CLSI (71.8%), detected by the disk diffusion method. This study is the first report to show patterns of susceptibility to five newly approved antibiotics among molecularly characterized isolates in Bulgaria. The data may contribute to both the improvement of treatment of individual patients and the choice of infection control strategy and antibiotic policy.
Application of Multi-Omics Technologies to the Study of Phytochromes in Plants
Authors: Wu Shumei , Gao Yue , Zhang Qi , Liu Fen , Hu Weiming
Published: January 2024

Phytochromes (phy) are distributed in various plant organs, and their physiological effects influence plant germination, flowering, fruiting, and senescence, as well as regulate morphogenesis throughout the plant life cycle. Reactive oxygen species (ROS) are a key regulatory factor in plant systemic responses to environmental stimuli, with an attractive regulatory relationship with phytochromes. With the development of high-throughput sequencing technology, omics techniques have become powerful tools, and researchers have used omics techniques to facilitate the big data revolution. For an in-depth analysis of phytochrome-mediated signaling pathways, integrated multi-omics (transcriptomics, proteomics, and metabolomics) approaches may provide the answer from a global perspective. This article comprehensively elaborates on applying multi-omics techniques in studying phytochromes. We describe the current research status and future directions on transcriptome-, proteome-, and metabolome-related network components mediated by phytochromes when cells are subjected to various stimulation. We emphasize the importance of multi-omics technologies in exploring the effects of phytochromes on cells and their molecular mechanisms. Additionally, we provide methods and ideas for future crop improvement.
C2CD4B Evokes Oxidative Stress and Vascular Dysfunction via a PI3K/Akt/PKCα–Signaling Pathway
Authors: Di Pietro Paola , Abate Angela Carmelita , Prete Valeria , Damato Antonio , Venturini Eleonora , Rusciano Maria Rosaria , Izzo Carmine , Visco Valeria , Ciccarelli Michele , Vecchione Carmine , Carrizzo Albino
Published: January 2024

High glucose–induced endothelial dysfunction is an important pathological feature of diabetic vasculopathy. While genome-wide studies have identified an association between type 2 diabetes mellitus (T2DM) and increased expression of a C2 calcium-dependent domain containing 4B (C2CD4B), no study has yet explored the possible direct effect of C2CD4B on vascular function. Vascular reactivity studies were conducted using a pressure myograph, and nitric oxide and oxidative stress were assessed through difluorofluorescein diacetate and dihydroethidium, respectively. We demonstrate that high glucose upregulated both mRNA and protein expression of C2CD4B in mice mesenteric arteries in a time-dependent manner. Notably, the inhibition of C2CD4B expression by genetic knockdown efficiently prevented hyperglycemia–induced oxidative stress, endothelial dysfunction, and loss of nitric oxide (NO) bioavailability. Recombinant C2CD4B evoked endothelial dysfunction of mice mesenteric arteries, an effect associated with increased reactive oxygen species (ROS) and decreased NO production. In isolated human umbilical vein endothelial cells (HUVECs), C2CD4B increased phosphorylation of endothelial nitric oxide synthase (eNOS) at the inhibitory site Thr495 and reduced eNOS dimerization. Pharmacological inhibitors of phosphoinositide 3-kinase (PI3K), Akt, and PKCα effectively attenuated oxidative stress, NO reduction, impairment of endothelial function, and eNOS uncoupling induced by C2CD4B. These data demonstrate, for the first time, that C2CD4B exerts a direct effect on vascular endothelium via a phosphoinositide 3-kinase (PI3K)/Akt/PKCα–signaling pathway, providing a new perspective on C2CD4B as a promising therapeutic target for the prevention of oxidative stress in diabetes–induced endothelial dysfunction.
The Amazonian Camu-Camu Fruit Modulates the Development of Drosophila melanogaster and the Neural Function of Adult Flies under Oxidative Stress Conditions
Authors: Musachio Elize Aparecida Santos , Pires Rafaela Garay , Fernandes Eliana Jardim , Andrade Stefani , Meichtry Luana Barreto , Janner Dieniffer Espinosa , Meira Graziela Moro , Ribeiro Euler Esteves , Barbisan Fernanda , Cruz Ivana Beatrice Mânica da , Prigol Marina
Published: January 2024

Camu-camu (Myrciaria dubia) is known for its antioxidant properties, although little is known about its developmental safety effects, particularly on adult neural function under basal redox and oxidative stress conditions. Therefore, this study sought to address this gap by conducting three complementary protocols using Drosophila melanogaster to investigate these effects. The initial assays revealed that second-stage larvae consumed diets supplemented with various concentrations of camu-camu uniformly, establishing a 50% lethal concentration at 4.799 mg/mL. Hence, non-lethal (0.1, 0.5, and 1 mg/mL) and sub-lethal (5 and 10 mg/mL) concentrations were then chosen to evaluate the effects of camu-camu on preimaginal development and adult neural function. Our observations showed that camu-camu impacts the expression of antioxidant enzymes, reactive species, and lipoperoxidation. Notably, sub-lethal concentrations decreased preimaginal viability and locomotor activity, negatively influenced geotaxis and acetylcholinesterase activity, and increased reactive species, catalase, and glutathione S-transferase activity in flies. Additionally, the protective effects of camu-camu against oxidative stress induced by iron (20 mM) were assessed. Flies supplemented with 0.5 mg/mL of camu-camu during the larval period showed improved neural viability and function, and this supplementation was found to protect against oxidative stress. These findings are instrumental in evaluating the safety and efficacy of commercial supplements based on camu-camu, offering significant insights for future research and application.
Pyrroloquinoline Quinone Improves Ram Sperm Quality through Its Antioxidative Ability during Storage at 4 °C
Authors: Zhu Zhendong , Li Wenjia , Yang Qitai , Zhao Haolong , Zhang Weijing , Adetunji Adedeji O. , Hoque S. A. Masudul , Kou Xin , Min Lingjiang
Published: January 2024

Sperm motility is an important factor in the migration of sperm from the uterus to the oviduct. During sperm preservation in vitro, sperm generates excessive ROS that damages its function. This study aims to investigate whether the addition of pyrroloquinoline quinone (PQQ) to the diluted medium could improve chilled ram sperm quality, and then elucidates the mechanism. Ram semen was diluted with Tris-citric acid-glucose (TCG) medium containing different doses of PQQ (0 nM, 10 nM, 100 nM, 1000 nM, 10,000 nM), and stored at 4 °C. Sperm motility patterns, plasma membrane integrity, acrosome integrity, mitochondrial membrane potential, reactive oxygen species (ROS) levels, malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity, and ATP levels were measured after preservation. Furthermore, the expressions of NADH dehydrogenase 1 (MT-ND1) and NADH dehydrogenase 6 (MT-ND6) in sperm were also detected by western blotting. In addition, sperm capacitation and the ability of sperm to bind to the zona pellucina were also evaluated. It was observed that the addition of PQQ significantly (p < 0.05) improved ram sperm motility, membrane integrity, and acrosome integrity during preservation. The percentage of sperm with high mitochondrial membrane potential in the PQQ treatment group was much higher than that in the control. In addition, supplementation of PQQ also decreased the sperm MDA and ROS levels, while increasing ATP levels. Interestingly, the levels of MT-ND1 and MT-ND6 protein in sperm treated with PQQ were also higher than that of the control. Furthermore, the addition of 100 nM PQQ to the medium decreased ROS damage in MT-ND1 and MT-ND6 proteins. The addition of 100 nM PQQ significantly (p < 0.05) increased protein tyrosine phosphorylation in ram sperm after induced capacitation. Furthermore, the value of the sperm–zona pellucida binding capacity in the 100 nM PQQ treatment group was also much higher than that of the control. Overall, during chilled ram- sperm preservation, PQQ protected ram sperm quality by quenching the ROS levels to reduce ROS damage and maintain sperm mitochondrial function, and preserved the sperm’s high ability of fertilization.