BIOCHEMICAL PARAMETERS CHANGES OF JAW BONE REMODELING IN EXPERIMENTAL ANIMALS UNDER THE INFLUENCE OF THE SUGGESTED PASTE, INTENDED FOR LOCAL TREATMENT OF CHRONIC GRANULATING PERIODONTITIS OF PERMANENT TEETH IN CHILDREN
Authors: Kostiuk І. R. , Kostiuk V. М. , Semenyuk H. D. 
Published: July 2021

The aim of the research was to study in an experiment on rats the biochemical parameters of the jaw bone remodeling under the influence of the developed paste, intended for the local treatment of chronic granulating periodontitis of permanent teeth in children.

The experimental research was performed on 36 young rats (males) of the Wistar line (130-150g body weight), which were kept in the vivarium. The animals were divided into 2 groups: an experimental and a control one - 18 rats each. They were operated in sterile conditions under ketamine anesthesia. After applying the alcohol to the skin, a scalpel was used to make an incision along the lower edge of the mandible in the area of the canines and a longitudinal incision of the buccal muscle, then access to the bone was bluntly created. Using a sharp dental probe, a bone defect was modeled, in which the animals of the experimental group were injected with a paste based on comfrey tincture and calcium hydroxide. The control group was operated without the application of paste, the bone defect healed under a blood clot. The surgical wound was sutured in layers with polyamide thread and treated with 1% alcohol solution of diamond green.

Blood sampling in experimental animals of both groups (6 rats each) was performed on 7th, 14th and 28th day after surgery. Animals were removed from the experiment by decapitation under ketamine anesthesia. Experimental animals were operated on and removed from the experiment during one day, under the same conditions.

Determination of the amount of calcium and inorganic phosphorus in the blood serum of rats was carried out using standard kits of research by "SIMKO Ltd” company, Lviv. The activity of acidic and alkaline phosphatases in the serum was determined using kits of research by BIO-Ld-TEST company, Lachem (Czech Republic) and "Simbj Ltd", Lviv, respectively. In order to determine these parameters in the serum of normal rats, we took blood from 6 healthy young animals that did not participate in the experiment and were on the diet of the vivarium.

The research found out that in the healing of artificial bone defects in the lower jaws of rats of the experimental group there were significant fluctuations in calcium and phosphorus levels and changes in the activity of acid phosphatase and alkaline phosphatase in the serum. The amount of Ca and P in the blood of rats decreased sufficiently. Alkaline phosphatase activity increased in both groups on the 7th and 14th day of the experiment, but the difference between the indicators in the groups was 38.35% (p <0.05) and 13.16% (p <0, 01) respectively. This was probably due to the rapid remission of inflammation at the site of surgery in the experimental group, as the paste we developed, which was used to fill a bone defect in this group of animals, has anti-inflammatory properties. Subsequent changes in alkaline phosphatase activity occurred similarly in both groups. This testified to the intensification of bone remodeling processes, as well as bone regeneration and mineralization under the influence of the paste we have developed.

Our biochemical researches of the blood of experimental animals confirmed the previously obtained results of morphological and densitometric studies and showed that the recovery of bone wounds of the lower jaws of rats occurs almost equally in the experimental and control groups, and paste based on comfrey tincture and calcium hydroxide promotes bone regeneration at the site of the defect and stimulates osteosynthesis.