bluuebunny/crossref_metadata_2025 huggingface dataset:
Macropropagation in Banana: Response to Hormone and Genome in Sucker Production
Authors: Challam Dawan Arkini , Mondal Subhasis , Mandi Sibu
Published: September 2023

Banana (Musa spp.) is the most important fruit crop of the world. The production of banana propagules for different scales of its cultivation by local farmers is still an important researchable issue as micropropagation is the only available source for plantlets production. An experiment on macro-propagation of banana belonging to three different genomic groups (AAA, AAB, ABB) was conducted at the Department of Plant Physiology, BCKV Mohanpur, West Bengal to study the induction of sucker through application of BAP @40 ppm. The substrate used for planting the corms were a mixture of sawdust + Trichoderma @ 15 g kg-1 of sawdust + vermicompost @ 15 g kg-1 of sawdust. Observations recorded were weight of the corm, days to appearance of the first primary suckers, number of primary suckers, number of secondary suckers, number of tertiary suckers and total number of suckers. The number of primary suckers induced ranged between 1.67 in Dwarf Cavendish (AAA) to 5.33 in Lacaton (AAA), while the number of secondary suckers induced ranged between 0.67 in Srimanti (AAA) to 22.00 in Manohar (AAB). The number of tertiary suckers induced were only observed in the genomic composition AAB with 4.00 and 0.67 in both the genotypes Madhurangbale and Manohar respectively. Days to bud appearance varies from 7.33 in Madhurangbale (AAA) to 29.33 days in Basrai (AAA). The weight of the corm also varied from 5.56 kg in Madhurangbale (AAA) and 2.06 kg in Kothia (ABB).
Incidence of Black Point on Wheat Seeds in Different Location of Uttarakhand, India
Authors: Kumar Vijendra , Kumar Varun , Rajesh  , Shakuntalala
Published: September 2023

An experiment was conducted to determine the incidence of black point disease in wheat varieties with different locations caused by A. alternata fungal pathogens of wheat grain. Fresh seeds of popularly cultivated wheat varieties, preferably viz., GW 366, GW 496, Lok 1, and GW 1, were collected after harvesting season from the farmers’ fields of wheat growing areas of middle Uttarakhand viz., Sahaspur, Roorkee, Haridwar, and Dehradun. At least 50 samples were collected from each of the districts. Five primary samples were collected and mixed from the harvested seed lot to have composite samples. Working samples were prepared from composite samples  by repeated halving method. The black point disease incidence might be due to the inherent boldness of the seeds. Florets remain more open in bold-seeded varieties, enhancing the chances of more seed infection during seed development. Moreover, low night temperatures  during the flowering period, severe dew at the seed maturation stage, frequent and heavy crop irrigation, and excessive application of nitrogenous fertilizers may lead to a higher incidence of the black point disease. Besides, infected seeds reveal seed rot, necrosis, and shriveling. High humidity due to prolonged rainfall or heavy dew deposition during seed maturation favors the black point. As our research findings varied, GW366 is significantly the highest incidence of black point disease.
Impact of Paper Mill Effluent on Groundnut Root Nodulation and Soil Microorganisms for Inclusion of Compost
Authors: Balamurugan R. , Dhanushkodi V. , Baskar M. , Rathika S. , UmaMaheswari T.
Published: September 2023

A Field experiment was conducted at Mondippatti village in Tiruchirappalli district of Tamil Nadu to study the effect of paper mill effluent irrigation with or without compost application on root growth, biological properties and soil nutrient status in Alfisol with groundnut as the test crop. The results revealed that the application of paper mill effluent along with compost favourably increased the soil nutrient status during the crop period. The highest concentrations of available nutrients viz., nitrogen, phosphorus, potassium, calcium, magnesium, sulphur, iron and zinc in the surface layer were 309.4 kg/ha, 14.2 kg/ha, 211.9 kg/ha, 64.9 meq/100g, 32.5 meq/100g, 38.8 mg/kg,11.4 mg/kg and 0.8 mg/kg, respectively in 75% paper mill effluent and press mud compost applications. Similarly, the use of paper mill effluent along with compost has a positive impact on soil microbial properties and highest population of bacteria was observed in 50% paper mill effluent and press mud application was 9.79 CFU (x106/g) followed by 100% paper mill effluent and press mud application (7.63 CFU (x106/g). While fungi and actinomycetes populations are higher in 75% paper mill effluent and farm yard manure application which were 5.64 CFU (x104/g) and 5.62 CFU (x104/g) respectively. The highest number of nodules was noticed in 100% paper mill effluent with press mud at 5 t/ha (45 numbers) followed by 75% paper mill effluent with press mud at 5 t/ha (42 numbers). From the study, it was concluded that the application of paper mill effluent with compost was found to be a promising source for soil fertility and root growth in groundnut.
New Records of Testate Amoebae in Shenthuriney Wildlife Sanctuary, Kerala, India: Insights into Microbial Biodiversity
Authors: L. Bindu.
Published: September 2023

This report summarizes the results hitherto achieved in the study of moss inhabitant testate amoebae in Shenthuriney WLS, part of Western Ghats, Kerala. This is the first study on this important protozoan group in the sanctuary to document the testate fauna.The study reveals the novel records of 28 species of testate amoebae span over 6 families and 9 genera from Shenthuriney WLS, Kerala.
Persistent Price Increase of Goods in Enugu State: Postgraduate Students of Business Education Perspective
Authors: Agbo Solomon, Uchenna , Obiageli Nnamani, Victoria
Published: September 2023

The main aim of the study was to determine the persistent price increase of goods in Enugu State: postgraduate students of business education perspective. The study contains three specific purposes. Also, the study was guided by three research questions and three hypotheses. The study adopted descriptive survey research design. The population of the study was 68 postgraduate students of Business Education with registration number ranging from 2019-2022. The sample size for the study was 58 which were obtained using Taro Yamane. The sampling technique used was convenient sampling technique. Producer Wholesaler Retailer Responsible Factors Questionnaire (PWRRFQ) was used as the study instrument. Face validation was done by three experts in the field of business education and measurement and evaluation. The internal consistent of the instrument was determined through the use of Cronbach Alpha and the results of 0.88 which shows that the instrument was highly reliable. Mean and standard deviation were used in analyzing the research questions while t-test statistic was used in analyzing the hypotheses that guided the study. The finding of the study shows that the Producers, the Wholesaler and Retailers are all responsible factors for the persistent increase in the prices of goods in Enugu State. The study recommended that government should subsidize fuel for businesses since all the three factors that were responsible for the persistent increases in the prices of goods lay causes on transportation cost of moving the goods. Finally, conclusion was drawn.
Um estudo sobre o sistema de sexo/gênero a partir da lógica filosófica e matemática
Authors: Puosso Cardoso Gouveia Costa Caio Jade
Published: September 2023

Este artigo tem como objetivo desenvolver uma aproximação entre a lógica filosófica e matemática clássica e o sistema de sexo/gênero hegemônico, orientado pelo paradigma binário homem/mulher. Proporemos a formalização de uma lógica de sexo/gênero clássica a fim de revelar como esse modelo cosmológico colonialista opera a partir dos mesmos princípios da lógica clássica, a saber, da identidade, da não-contradição e do terceiro excluído. Por conseguinte, avaliaremos como certas vidas, nomeadas atualmente como ‘trans’, provocam explosões e colapsos nesses sistemas lógicos clássicos. Assim, indicaremos que essas existências remontam diversas rotas culturais que têm sido apagadas e perseguidas pelo sistema colonialista, mas que ainda podem ser retomadas e revividas.
Afetos marcando o corpo
Authors: De Lacerda Gustavo Haiden
Published: September 2023

Nem todo corpo nem todo afeto pode circular do mesmo modo dentro de uma determinada sociedade em dado momento histórico. Quando tratamos das (im)possibilidades de representação na arte, perguntamos pelo funcionamento de seu regime estético, ligado às condições de produção dos discursos e das subjetividades. Neste artigo, refletimos acerca do processo de re-partilha do sensível em obras fotográficas de Hujar e de Wojnarowicz, partindo dos estudos de Rancière (2009), Courtine (2013) e Safatle (2015). Buscamos mostrar, pela análise das fotografias, a maneira pela qual o corpo (gay) comparece artisticamente nas obras em questão e como essas produções reconfiguram os campos do sensível e do (in)visível, permitindo a constituição de outros corpos e outros afetos.
RESENHA: Histórias LGBTI+
Authors: Vieira Filho Maurício João , Desidério Ricardo
Published: September 2023

Resenha da obra Movimento LGBTI+: uma breve história do século XIX aos nossos dias, publicado em 2022, por Renan Quinalha, cujo propósito se volta para capturar silenciamentos e projetos de esquecimento das diversidades sexuais e de gênero para, assim, catapultar esse movimento político à cena pública atual. Organizada em cinco capítulos, o autor discorre sobre ativismos políticos organizados na Alemanha do século XIX, Estados Unidos pós-Segunda Guerra Mundial para chegar ao cenário brasileiro de hoje. Urgente e provocadora, a obra se lança ao desafio de aparecer frente ao projeto de ocultamento conservador e gritar diante das violências cotidianas enfrentadas por nós. Aberto para públicos da academia e para além dela, o livro chega em um momento chave no país e se configura em um convite para alianças e comunhões de re-existências e resistências.
Os estudos queer e os (cu)ir dos estudos
Authors: Barone María Antonella , Rodrigues Alexsandro , Lima de Souza Mariane
Published: September 2023

Pode-se registrar uma emergência do campo dos estudos queer a partir da década de 1980, localizado no conservadorismo dos Estados Unidos e na germinação de movimentos sociais que se colocaram na reivindicação de questões relacionadas à epidemia de HIV/AIDS nessa década. Sua articulação teórica reformulou os conceitos de sexo e gênero, oferecendo instrumentos para aprimorar a compreensão deste campo. No presente estudo, colocamos em tensão tal campo epistémico, com as narrativas e os saberes do “cu do mundo”, a partir das vivências de pessoas trans e travestis que oferecem pistas para empreender os caminhos tortos e desviantes pelos quais é possível acessar outros recursos hermenêuticos, no que diz respeito a gênero e sexualidades.
Contribuições decoloniais para a produção de conhecimento por pesquisadoras em contextos periféricos
Authors: Queiroz Isabela Saraiva de , Toneli Maria Juracy Filgueiras
Published: September 2023

Este ensaio apresenta algumas contribuições decoloniais para a metodologia de pesquisa, questionando as dicotomias presentes na produção de conhecimento, por meio do reconhecimento de uma geopolítica do saber tecida a partir de territórios existenciais forjados no seio da ferida colonial. Sugere-se que o exercício do lugar de pesquisadora em contextos periféricos se dá quando feito de forma atenta à sua impossibilidade, ou seja, da posição de “forasteira de dentro”, por meio da descolonização dos afetos e da desobediência epistêmica, a partir de um terreno epistemológico no qual o pensamento de fronteira é exercido.
RESENHA: As políticas do desejo
Authors: Lins Paola
Published: September 2023

Livro resenhado: SRINIVASAN, Amia. O direito ao sexo. Feminismo no século vinte e um. São Paulo: Todavia, 2021.
Mental Health Outcomes Among Patients Living in US Counties Lacking Broadband Access and Psychiatrists
Authors: Ramesh Tarun , McBain Ryan K. , Cantor Jonathan H. , Stein Bradley D. , Yu Hao
Published: September 2023

This cross-sectional study identifies the prevalence of counties without psychiatrists and broadband coverage, describes their sociodemographic characteristics, and quantifies their mental health outcomes.
The Weight of Inevitability—My Journey With BRCA2
Authors: Wu Katherine P.
Published: September 2023

In this essay, the author grapples with the possibility of a diagnosis of BRCA2.
Molecular Auxetic Polymer of Intrinsic Microporosity via Conformational Switching of a Cavitand Crosslinker
Authors: Portone Francesca , Amorini Mattia , Montanari Matteo , Pinalli Roberta , Pedrini Alessandro , Verucchi Roberto , Brighenti Roberto , Dalcanale Enrico
Published: September 2023

Auxetics are materials characterized by a negative Poisson's ratio (NPR), an uncommon mechanical behavior corresponding to a transversal deformation tendency opposite to the traditional materials. Here, the first example of a synthetic molecular auxetic polymer obtained by embedding a conformationally expandable cavitand as a crosslinker into a rigid polymer of intrinsic microporosity (PIM) is presented. The rigidity and microporosity of the polymeric matrix are pivotal to maximizing the expansion effect of the cavitand that, under mechanical stress, can assume two different conformations: a compact vase one and an extended kite form. The auxetic behavior and the corresponding NPR of the proposed material is predicted by a specific micromechanical model that considers the cavitand volume expansion ratio, the fraction of the cavitand crosslinker in the polymer, and the mechanical characteristics of the polymer backbone. The reversible auxetic behavior of the material is experimentally verified via the digital image correlation technique performed during the mechanical tests on films obtained by blending the auxetic crosslinked polymer with pristine PIM. Two specific control experiments prove that the mechanically driven conformational expansion of the cavitand crosslinker is the sole responsible for the observed NPR of the polymer.
Design Principles of Single‐Atom Catalysts for Oxygen Evolution Reaction: From Targeted Structures to Active Sites
Authors: Jiang Fei , Li Yichuan , Pan Yuan
Published: September 2023

Hydrogen production from electrolytic water electrolysis is considered a viable method for hydrogen production with significant social value due to its clean and pollution‐free nature, high hydrogen production efficiency, and purity, but the anode oxygen evolution reaction (OER) process is complex and kinetically slow. Single‐atom catalysts (SACs) with 100% atom utilization and homogeneous active sites often exhibit high catalytic activity and are expected to be extensively applied. The catalytic performance of OER can be further improved by precise regulation of the structure through electronic effects, coordination environment, heteroatomic doping, and so on. In this review, the mechanisms of OER under different conditions are introduced, the latest research progress of SACs in the field of OER is systematically summarized, and then the effects of various structural regulation strategies on catalytic performance are discussed, and principles and ideas for the design of SACs for OER are proposed. In the end, the outstanding issues and current challenges in this field are summarized.
Halide Perovskites‐Based Diffusive Memristors for Artificial Mechano‐Nociceptive System
Authors: Im In Hyuk , Baek Ji Hyun , Kim Seung Ju , Kim Jaehyun , Park Sung Hyuk , Kim Jae Young , Yang J. Joshua , Jang Ho Won
Published: September 2023

Numerous efforts for emulating organ systems comprised of multiple functional units have driven substantial advancements in bio‐realistic electronics and systems. The resistance change behavior observed in diffusive memristors shares similarities with the potential change in biological neurons. Here, the diffusive threshold switching phenomenon in Ag‐incorporated organometallic halide perovskites is utilized to demonstrate the functions of afferent neurons. Halide perovskites‐based diffusive memristors show a low threshold voltage of ≈0.2 V with little variation, attributed to the facile migration of Ag ions uniformly dispersed within the halide matrix. Based on the reversible and reliable volatile threshold switching, the memristors successfully demonstrate fundamental nociceptive functions including threshold firing, relaxation, and sensitization. Furthermore, to replicate the biological mechano‐nociceptive phenomenon at a system level, an artificial mechano‐nociceptive system is built by integrating a diffusive memristor with a force‐sensing resistor. The presented system is capable of detecting and discerning the detrimental impact caused by a heavy steel ball, effectively exhibiting the corresponding sensitization response. By further extending the single nociceptive system into a 5 × 5 array, successful stereoscopic nociception of uneven impulses is achieved in the artificial skin system through array‐scale sensitization. These results represent significant progress in the field of bio‐inspired electronics and systems.
Judicious Selection of Precursors with Suitable Chemical Valence State for Controlled Growth of Transition Metal Chalcogenides
Authors: Lan Shangui , Zhang Zhixiang , Hong Yukun , She Yihong , Pan Baojun , Xu Yang , Wang Peijian
Published: September 2023

Transition metal chalcogenides (TMCs) have attracted wide attentions as a class of promising material for both fundamental investigations and electronic applications due to their atomic thin thickness, dangling bond‐free surface, and excellent electronic properties. Specifically, TMCs show outstanding properties such as good thermal conductivity, robust mechanical properties, and extraordinary electronical characteristics, bestowing them utility in both fundamental research and applications. Recently, the development of post‐Moore electronics based on TMCs calls for their large‐size and single‐crystal growth. However, researchers about synthesis usually focus on controlling several growth parameters (such as growth temperature, flow rate, and time). Herein, it is reported that the chemical valence states of transition metal precursors play an important role in controlling the lateral size and crystal quality for TMCs. The study discusses the valence states‐dependent growth mechanism for WS2 and MoS2 from four factors: evaporation temperature, skipping of reaction steps, atomic binding energy of the precursors, and formation energy. In addition, the as‐grown WS2 and MoS2 nanoflakes exhibit good photoelectric response properties. For EuS, the growth results are obviously different by using EuBr3 and EuBr2 as precursors. The studies provide a unique perspective and also new knowledge to controllably grow large‐size and good crystal quality TMCs.
Modular Optical Diodes for Circular Polarization Based on Glass‐Supported Cellulose Nanocrystal/Polyethylene Glycol Composite Films
Authors: Mendoza-Galván Arturo , Juárez-Rivera Olga Rubi , Mauricio-Sánchez Reina Araceli , Järrendahl Kenneth , Arwin Hans
Published: September 2023

Depending on processing methods and conditions, cellulose nanocrystal (CNC) films exhibit linear birefringence or selective Bragg reflection. The latter means reflection of light with left‐handed circular polarization (LCP) due to CNC in a helicoidal microstructure of the same handedness. Herein, glass‐supported CNC/polyethylene glycol (CNC/PEG) composite films with PEG concentrations in the range of 0–30% w/w with selective Bragg reflection at wavelengths from 440 to 550 nm are prepared. A modular device comprised of a dip‐coated birefringent CNC‐glass sample sandwiched between two CNC/PEG‐glass samples shows different responses to light with LCP and to light with right‐handed circular polarization (RCP). The device suppresses selective Bragg reflection from the rear (front) CNC/PEG sample for incident light with LCP (RCP), even when the birefringent film does not meet the condition for a halfwave plate. This behavior resembles the performance of optical diodes for circular polarization. Polarization properties of composite films and optical diodes in terms of degree of polarization and ellipticity are discussed within the Stokes–Mueller formalism. Electromagnetic simulations of Mueller matrices reveal the equivalence of modular and in‐tandem film approaches of optical diodes.
Copper‐Catalyzed Photoinduced [3+3] Cycloaddition Reactions of α‐Acidic Isocyanides with Tetrazoles
Authors: Liu Hua‐Wei , Hao Yong‐Jiu , Cai Zhong‐Jian , Ji Shun‐Jun
Published: September 2023

Herein, a copper‐catalyzed photoinduced [3+3] cycloaddition reaction of α‐acidic isocyanides with 2,5‐diaryltetrazoles is described. The reaction proceeded smoothly with high regioselectivity, affording a range of important 1,2,4‐triazines under mild conditions. The obtained 1,2,4‐triazines were converted into triazine alcohol, Weinreb amide and triazinone easily, which further illustrate the utility of this [3+3] cycloaddition process.
Rapid and Safe Continuous‐Flow Simmons‐Smith Cyclopropanation using a Zn/Cu Couple Column
Authors: Nova‐Fernández José Luis , Pascual‐Coca Gustavo , Cabrera Silvia , Alemán José
Published: September 2023

Flow chemistry has recently opened new chemistry windows thanks to the safer use of hazardous and sensitive reagents. Furthermore, flow procedures usually outperform their batch counterparts due to improved mass and heat transfer, offering a good opportunity for industrial application. Herein, a rapid Simmons‐Smith cyclopropanation flow process is presented. Taking advantage of the in situ production of the zinc carbenoid species, several olefins bearing aromatic rings of different electronic nature, aliphatic chains or heterocycles were smoothly cyclopropanated with a residence time of just 15 minutes. In addition, industrial applicability of the protocol is assured thanks to a successful 12‐mmol scale experiment, which represents a 3.59 grams per hour production of a selected example, and to the satisfactory synthesis of pharmaceutical drugs.
An Aqueous Three‐Component Reaction of Cyclopropanols, DABCO ⋅ (SO2)2 and Diaryliodonium salts
Authors: Qiu Yanjie , Yao Jiating , Xia Hongguang , Zhu Chumeng , Ye Shengqing , Wu Jie , Chen Qi
Published: September 2023

An additive‐ and external oxidant‐free reaction of cyclopropanols, DABCO ⋅ (SO2)2 and diaryliodonium salts in aqueous phase is described. This reaction proceeds under mild reaction conditions, affording bioactive aryl substituted γ‐keto sulfones in 36%‐90% yields with good functional group tolerance. This reaction proceeds through a γ‐keto sulfinate intermediate, which undergoes a nucleophilic reaction with diaryliodonium salts, giving rise to corresponding aryl substituted γ‐keto sulfones.
Functionally Tailored Metal–Organic Framework Coatings for Mediating Ti Implant Osseointegration
Authors: Zhang Yuan , Cheng Zhuo , Liu Zaiyang , Shen Xinkun , Cai Chunyuan , Li Menghuan , Luo Zhong
Published: September 2023

Owing to their mechanical resilience and non‐toxicity, titanium implants are widely applied as the major treatment modality for the clinical intervention against bone fractures. However, the intrinsic bioinertness of Ti and its alloys often impedes the effective osseointegration of the implants, leading to severe adverse complications including implant loosening, detachment, and secondary bone damage. Consequently, new Ti implant engineering strategies are urgently needed to improve their osseointegration after implantation. Remarkably, metalorganic frameworks (MOFs) are a class of novel synthetic material consisting of coordinated metal species and organic ligands, which have demonstrated a plethora of favorable properties for modulating the interfacial properties of Ti implants. This review comprehensively summarizes the recent progress in the development of MOF‐coated Ti implants and highlights their potential utility for modulating the bio‐implant interface to improve implant osseointegration, of which the discussions are outlined according to their physical traits, chemical composition, and drug delivery capacity. A perspective is also provided in this review regarding the current limitations and future opportunities of MOF‐coated Ti implants for orthopedic applications. The insights in this review may facilitate the rational design of more advanced Ti implants with enhanced therapeutic performance and safety.
Interface Modification by Ammonium Sulfamate for High‐Efficiency and Stable Perovskite Solar Cells
Authors: Cao Yang , Feng Jiangshan , Wang Mingzi , Yan Nan , Lou Junjie , Feng Xiaolong , Xiao Fengwei , Liu Yucheng , Qi Danyang , Yuan Yin , Zhu Xuejie , Liu Shengzhong (Frank)
Published: September 2023

Defects in perovskite films are still the dominant destroyer of both power conversion efficiency (PCE) and long‐term stability in perovskite solar cells (PSCs). As the most popular electron transport layer (ETL), TiO2 film is used in many PSCs to achieve high PCE. However, pristine TiO2 by itself is not sufficient as an ETL due to lattice mismatch, poor alignment of the energy level gap, and hysteresis of the PSC. Herein, ammonium sulfamate (AS), with desired NH4+ and S═O functional groups, is designed to modify the TiO2 surface and interface to improve the PCE of PSCs. It is found that the AS works like a seed layer for the perovskite deposition, and, in addition, it effectively forms a bridge between the TiO2 surface and the perovskite. As a result, PSCs are successfully fabricated with a champion power conversion efficiency of 24.78% with smaller hysteresis. The PSCs prepared using the AS‐modified TiO2 also show excellent stability, and the bare device without any encapsulation retains 96% of its initial PCE after 1056 h of ambient exposure at 25 °C and 25% relative humidity.
Children's rights violations: Parental corporal punishment under the microscope
Authors: Greeff Laetitia‐Ann
Published: September 2023

When Australia ratified the United Nations Convention on the Rights of the Child (the Convention) in 1990, all children within its borders became entitled to the protection of the rights set out in the Convention. Children, thus, became rights holders. More than just human rights holders, they became children's rights holders. The Convention is the first international instrument to address the protection of children from violence directly. Corporal punishment (CP) is increasingly recognised internationally as physical abuse and a violation of children's rights. However, CP in the home is still legal in all Australian jurisdictions even though it violates several of the rights protected by the Convention. This contribution will examine Articles 2, 3(1), 5, 16, 19(1), 24(3) and 37(a) of the Convention to outline how parental CP undermines the legally binding obligations of the Convention by violating the rights espoused by these provisions. This article then calls for CP to be outlawed by all state and territory governments to adhere to the aim of the Convention that because of children's unique vulnerabilities, all their rights need to be protected, respected and promoted by those who ratified the Convention.
Prediction of Interesting Ferromagnetism in Janus Semiconducting Cr2AsP Monolayer
Authors: Ma Qiuyue , Li Yingmei , Ge Yanfeng , Yang Guochun , Liu Yong
Published: September 2023

2D half‐metallic materials that have sparked intense interest in advanced spintronic applications are essential to the developing next‐generation nanospintronic devices. This study has adopted a first‐principles calculation method to predict the magnetic properties of intrinsic, Se‐doped, and biaxial strain tuning Cr2AsP monolayer. The Janus Cr2AsP monolayer is proven to be an intrinsic ferromagnetic (FM) semiconductor with an exchange splitting bandgap of 0.15 eV at the PBE+U level. Concentration‐dependent Se doping, such as Cr2AsSexP (x = 0.25, 0.50, 0.75), can regulate Cr2AsP from FM semiconductor to FM half‐metallicity. Specifically, the spin‐up channel crosses the Fermi level, while the spin‐down channel has a bandgap. More interestingly, the wide half‐metallic bandgaps and spin bandgaps make them have important implications for the preparation of spintronic devices. At last, it also explore the effect of biaxial strain from ‐14% to 10% on the magnetism of the Cr2AsP monolayer. There appears a transition from FM to antiferromagnetic (AFM) at a compressive strain of ‐10.7%, originating from the competition between the indirect FM superexchange interaction and the direct AFM interaction between the nearest neighboring Cr atoms. Additionally, when the compressive strain is ‐2% or the tensile strain is 6%, the semiconducting Cr2AsP becomes a half‐metallic material. These charming properties render the Janus Cr2AsP monolayer with great potential for applications in spintronic devices.
Display‐Selektion eines Foldamer‐Peptid‐Makrozyklus‐Hybriden
Authors: Dengler Sebastian , Howard Ryan T. , Morozov Vasily , Tsiamantas Christos , Huang Wei‐En , Liu Zhiwei , Dobrzanski Christopher , Pophristic Vojislava , Brameyer Sophie , Douat Céline , Suga Hiroaki , Huc Ivan
Published: September 2023

Die Erweiterung der chemischen Vielfalt von Peptid‐Makrozyklenbibliotheken für die Display‐Selektion ist wünschenswert, um ihr Potenzial zur Bindung an biomolekulare Ziele zu verbessern. Wir haben nun eine erhebliche Erweiterung durch die Einbeziehung eines großen, aromatischen helikalen Foldamers umgesetzt. Es wurde ein helikales aromatisches Foldamer identifiziert, welches eine Flexizym‐vermittelte tRNA‐Acylierung durchläuft und in der Lage ist, die ribosomale Translation mit Ausbeuten durchzuführen, die ausreichend hoch sind, um eine mRNA‐Display‐Selektion von makrozyklischen‐Foldamer‐Peptid‐Hybriden zu vollziehen. Ein hybrider Makrozyklus mit nanomolarer Affinität an C‐lobe der E6AP HECT‐Domäne wurde ausgewählt, der eine stark konvergente Peptidsequenz aufwies. Eine Kristallstruktur und Moleküldynamik‐Simulationen haben gezeigt, dass sowohl das Peptid als auch das Foldamer in einer faszinierenden, reziproken Heftklammer‐artigen Art helikal sind. Die starke Konvergenz der Reste könnte durch ihre Beteiligung an spezifischen Wechselwirkungen mit dem Zielprotein erklärt werden. Das Foldamer stabilisiert die Peptidhelix durch Heftklammern und durch Kontakte mit wesentlichen Resten. Diese Ergebnisse stellen insgesamt eine erhebliche Erweiterung des chemischen Raums dar, der für die Display‐Selektion zur Verfügung steht, und unterstreichen die möglichen Vorteile des Einfügens eines aromatischen Foldamers in einen Peptidmakrozyklus zum Zwecke der Proteinerkennung.
A Nanomedicine‐Enabled Ion‐Exchange Strategy for Enhancing Curcumin‐Based Rheumatoid Arthritis Therapy
Authors: Yang Jiacai , Yang Bowen , Shi Jianlin
Published: September 2023

Curcumin (Cur) has been clinically used for rheumatoid arthritis treatment by the means of reactive oxygen species (ROS) scavenging and immune microenvironment regulation. However, this compound has a poor water solubility and moderate antioxidative activity, favoring no further broadened application. Metal complexes of curcumin such as zinc‐curcumin (Zn−Cur) features enhanced water solubilities, while copper‐curcumin (Cu−Cur) shows a higher antioxidant activity but lower solubility than Zn−Cur. Based on their inherent biological properties, this work proposes a nanomedicine‐based ion‐exchange strategy to enhance the efficacy of Cur for rheumatoid arthritis treatment. Copper silicate nanoparticles with hollow mesoporous structure were prepared to load water‐soluble Zn−Cur for constructing a composite nanomedicine, which can degrade in acidic microenvironment of arthritic region, releasing Cu2+ and Zn−Cur. Cu2+ then substitute for Zn2+ in Zn−Cur to form Cu−Cur with a significantly enhanced antioxidative effect, capable of efficiently scavenging ROS in M1 macrophages, promoting their transition to an anti‐inflammatory M2 phenotype. In addition, the silicate released after nanocarrier degradation and the Zn2+ released after ion exchange reaction synergistically promote the biomineralization of osteoblasts. This work provides a new approach for enhancing the antiarthritic effect of Cur via an ion‐exchange strategy.
Display Selection of a Hybrid Foldamer–Peptide Macrocycle
Authors: Dengler Sebastian , Howard Ryan T. , Morozov Vasily , Tsiamantas Christos , Huang Wei‐En , Liu Zhiwei , Dobrzanski Christopher , Pophristic Vojislava , Brameyer Sophie , Douat Céline , Suga Hiroaki , Huc Ivan
Published: September 2023

Expanding the chemical diversity of peptide macrocycle libraries for display selection is desirable to improve their potential to bind biomolecular targets. We now have implemented a considerable expansion through a large aromatic helical foldamer inclusion. A foldamer was first identified that undergoes flexizyme‐mediated tRNA acylation and that is capable of initiating ribosomal translation with yields sufficiently high to perform an mRNA display selection of macrocyclic foldamer–peptide hybrids. A hybrid macrocyclic nanomolar binder to the C‐lobe of the E6AP HECT domain was selected that showed a highly converged peptide sequence. A crystal structure and molecular dynamics simulations revealed that both the peptide and foldamer are helical in an intriguing reciprocal stapling fashion. The strong residue convergence could be rationalized based on their involvement in specific interactions with the target protein. The foldamer stabilizes the peptide helix through stapling and through contacts with key residues. These results altogether represent a significant extension of the chemical space amenable to display selection and highlight possible benefits of inserting an aromatic foldamer into a peptide macrocycle for the purpose of protein recognition.
Application of polysaccharide based modified ground calcium carbonate in wet‐end of papermaking
Authors: Chatterjee Trinadha , Negi Satya Dev , Janbade Anuradha , Tyagi Sanjay , Gupta Manoj Kumar , Bhardwaj Shubhang , Kaur Prabhjot
Published: September 2023

To enhance the ash in paper without reducing its strength property, ground calcium carbonate (GCC) filler was modified using polysaccharide prior to its addition at wet‐end of papermaking. Till now, modification of GCC filler using chitosan acetate (CH.A) is not explored. The novelty lies in showing the effect of CH.A as GCC filler modifier and its application in papermaking. The results showed that ash of paper get increased by around 18%, 53% and 63% after adding GCC modified using cationic starch (C.S) @ 20 kg/t of GCC, CH.A @ 5 kg/t of GCC and CH.A @ 10 kg/t of GCC respectively, in comparison to that attained by paper using unmodified GCC at the same dose at wet‐end of papermaking. The breaking length, burst factor and tear factor of paper having 11% ash (made using unmodified GCC) was found to be 2485 m, 21.73 and 47.63, respectively. The paper having 18% ash (made using GCC modified by CH.A 10 kg/t of GCC) showed breaking length, burst factor and tear factor as 2364 m, 22.24 and 46.72, respectively. This approach of using CH.A in papermaking will help the papermaker to improve profit and quality of paper.
Site‐specific crosslinking and assembly of tetrameric β‐glucuronidase improve glycyrrhizin hydrolysis
Authors: Wang Qibin , Wang Yingying , Jian Xing , Wang Ning , Li Chun , Liu Hu
Published: September 2023

In this study, eight nonconserved residues with exposed surfaces and flexible conformations of the homotetrameric PGUS (β‐glucuronidase from Aspergillus oryzae Li‐3) were identified. Single‐point mutation into cysteine enabled the thiol‐maleimide reaction and site‐specific protein assembly using a two‐arm polyethylene glycol (PEG)‐maleimide crosslinker (Mal2). The Mal2(1k) (with 1 kDa PEG spacer)‐crosslinked PGUS assemblies showed low crosslinking efficiency and unimproved thermostability except for G194C‐Mal2(1k). To improve the crosslinking efficiency, a lengthened crosslinker Mal2(2k) (with 2 kDa PEG spacer) was used to produce PGUS assembly and a highly improved thermostability was achieved with a half‐life of 47.2–169.2 min at 70°C, which is 1.04–3.74 times that of wild type PGUS. It is found that the thermostability of PGUS assembly was closely associated with the formation of inter‐tetramer assembly and intratetramer crosslinking, rather than the PEGylation of the enzyme. Therefore, the four‐arm PEG‐maleimide crosslinker Mal4(2k) (with 2 kDa PEG spacer) was employed to simultaneously increase the inter‐tetramer assembly and intratetramer crosslinking, and the resulting PGUS assemblies showed further improved thermostabilities compared with Mal2(2k)‐crosslinked assemblies. Finally, the application of PGUS assemblies with significantly improved thermostability to the bioconversion of GL proved that the PGUS assembly is a strong catalyst for glycyrrhizin (GL) hydrolysis in industrial applications.
Enhancing green brand equity through environmental reputation: The important of green brand image, green brand trust, and green brand loyalty
Authors: Tran Nguyen Khanh Hai
Published: September 2023

While previous research have established the advantages of environmental reputation (ER) in the realm of brand development, this article examines the role of ER on the value of green brands in the green food production industry, based on customer perceptions in a newly emerging market, using insights from signal theory and the Hierarchy of Effects Model (HEM). The study data was obtained from consumers who regularly make purchasing decisions for green‐labeled food in Vietnam. The study results suggest that the ER is a critical factor to develop green brand equity (GBE). This is achieved by developing the green brand image (GBI), establishing the green brand trust (GBT), and fostering the green brand loyalty (GBL). The results provide a significant contribution to promoting the development of green brand value in customers' awareness by encouraging activities and programs that enhance ER to strengthen GBI, GBT, and GBL towards green food brands. In addition to the findings, the research has limitations and suggests future research directions.
Current insights into the oncogenic roles of lncRNA LINC00355
Authors: Shen Jinze , Su Xinming , Pan Ming , Wang Zehua , Ke Yufei , Wang Qurui , Dong Jingyin , Duan Shiwei
Published: September 2023

Long noncoding RNAs (lncRNAs) are a class of nonprotein‐coding transcripts that are longer than 200 nucleotides. LINC00355 is a lncRNA located on chromosome 13q21.31 and is consistently upregulated in various cancers. It regulates the expression of downstream genes at both transcriptional and posttranscriptional levels, including eight microRNAs (miR‐15a‐5p, miR‐34b‐5p, miR‐424‐5p, miR‐1225, miR‐217‐5p, miR‐6777‐3p, miR‐195, and miR‐466) and three protein‐coding genes (ITGA2, RAD18, and UBE3C). LINC00355 plays a role in regulating various biological processes such as cell cycle progression, proliferation, apoptosis, epithelial‐mesenchymal transition, invasion, and metastasis of cancer cells. It is involved in the regulation of the Wnt/β‐catenin signaling pathway and p53 signaling pathway. Upregulation of LINC00355 has been identified as a high‐risk factor in cancer patients and its increased expression is associated with poorer overall survival, recurrence‐free survival, and disease‐free survival. LINC00355 upregulation has been linked to several unfavorable clinical characteristics, including advanced tumor node metastasis and World Health Organization stages, reduced Karnofsky Performance Scale scores, increased tumor size, greater depth of invasion, and more extensive lymph node metastasis. LINC00355 induces chemotherapy resistance in cancer cells by regulating five downstream genes, namely HMGA2, ABCB1, ITGA2, WNT10B, and CCNE1 genes. In summary, LINC00355 is a potential oncogene with great potential as a diagnostic marker and therapeutic target for cancer.
LRRC59 serves as a novel biomarker for predicting the progression and prognosis of bladder cancer
Authors: Zhang Peng , Xie Xiaodu , Li Chunming , Zhang Chaohua , Liang Peihe
Published: September 2023

Background

Leucine‐rich repeat‐containing protein 59 (LRRC59) is an endoplasmic reticulum membrane protein involved in various cancers, but its role in bladder cancer (BC) has not been reported. The aim of the present study was to investigate the role of LRRC59 protein in BC progression and prognosis.

Methods

The expression profile and clinical significance were retrieved from BC patients in the Cancer Genome Atlas database. The methylation status of LRRC59 was analyzed by UALCAN and MethSurv databases. Potential signaling pathways and biological functions were explored by functional enrichment analysis. Immunocyte infiltration was evaluated by CIBERSORT analysis. The prognostic value of LRRC59 was evaluated by Kaplan–Meier and Cox regression analyses. Overall survival (OS) was predicted by the nomogram plot established in this study. LRRC59 expression in 10 pairs BC and adjacent noncancerous tissues were analyzed by immunohistochemistry (IHC). Cell proliferation, migration, and invasion were detected by CCK8, colony formation assay, transwell assay, and cell scratch assay, respectively. Proteins related to epithelial–mesenchymal transition and apoptosis were detected by western blot.

Results

LRRC59 overexpression significantly decreased OS, disease‐specific survival, and progress‐free interval of BC patients. LRRC59 was a prognostic marker for OS and its hypomethylation status signified a poor prognosis. LRRC59 overexpression was correlated with infiltration of resting memory CD4 T cells, memory activated CD4 T cells, resting NK cells, macrophages M0, M1, M2, and neutrophils. IHC showed that the LRRC59 expression in BC tissue was significantly higher than that in adjacent noncancerous tissue. Knockdown of LRRC59 expression inhibited the proliferation of BC cells and reduced their migratory ability. Western blot showed that Snail and vimentin protein expressions decreased, while E‐cadherin expressions increased.

Conclusions

LRRC59 expression can predict the outcome of BC independently and serve as a new biomarker for diagnosis.
Design, Synthesis of Novel 1,2,3‐Triazole Pendent Quinazolinones and Their Cytotoxicity against MCF‐7 Cell Line
Authors: Myakala Nagaraju , Kandula Kotaiah , Rayala Nagamani , Kuna Sateesh , Thumma Vishnu , Durga Bhavani Anagani Kanaka
Published: September 2023

A library of 6‐(((1‐(substitutedphenyl)‐1H‐1,2,3‐triazol‐4‐yl)methyl) amino)‐3‐methylquinazolin‐4(3H)‐one analogues synthesized from Isatin precursor through a series of nitration, reduction, hydrolysis, cyclization and click reaction. The structures of compounds were characterized by spectral data including IR, 1H‐NMR, 13C NMR and Mass. The novel quinazolinone – 1,2,3‐triazoles were screened for their cytotoxicity against the human breast adenocarcinoma cell lines MCF‐7 by MTT assay. 4‐Isopropyl and 2‐bromo substituted analogues executed high activity against MCF‐7 cell line with IC50 value of 10.16±0.07 μM and 11.23±0.20 μM compared to the Doxorubicin whose IC50 value is 10.81±0.03 μM. The activity of remaining compounds is good to moderate. Further, the molecular docking studies against the crystal structure of Epidermal Growth Factor Receptor delivered the best binding energies and the interactions such as H‐bond and hydrophobic are inevitable. The predicted pharmacokinetic properties results showed that these compounds have more drug likeness properties.
Transforming growth factor β1 upregulates 6‐phosphofructo‐2‐kinase/fructose 2,6‐bisphosphatase‐4 expression in A549 and MCF‐10A cells
Authors: Altunok Tugba H. , Muchut Robertino J. , Iglesias Alberto A. , Yalcin Abdullah
Published: September 2023

Transforming growth factor β1 (TGFβ1) induces a cellular process known as epithelial–mesenchymal transition (EMT) associated with metabolic reprogramming, including enhanced glycolysis. Given the involvement of 6‐phosphofructo‐2‐kinase/fructose 2,6‐bisphosphatase (PFKFB) enzymes in glycolysis, we aimed to investigate whether TGFβ1 regulates expressions of PFKFB genes and if PFKFBs are required for TGFβ1‐driven phenotypes. A549 and MCF‐10A cell lines were used as TGFβ1‐driven EMT models. Messenger RNA expressions of PFKFB and EMT genes were determined by real‐time quantitative polymerase chain reaction. A small interfering RNA approach was used to deplete PFKFB4 expression. A Matrigel invasion assay was conducted to assess the effect of PFKFB4 silencing on the TGFβ1‐enhanced invasion of A549 cells. F2,6BP levels were analyzed using an enzyme‐coupled assay. Glucose and lactate concentrations were determined using colorimetric assays. TGFβ1 robustly induced expression of the fourth isoform of PFKFBs, PFKFB4, in both cell lines. PFKFB4 depletion partially inhibits mesenchymal transdifferentiation caused by TGFβ1 in A549 cells, as assessed by microscopy. Inductions of Snail in MCF‐10A cells and Fibronectin in A549 cells and repressions of E‐cadherin in both cell lines by TGFβ1 are attenuated by PFKFB4 silencing. PFKFB4 silencing reduces F2,6BP and glycolytic activity, although TGFβ1 alone does not affect these parameters. Finally, PFKFB4 depletion suppresses the TGFβ1‐driven invasion of A549 cells through Matrigel. Presented data suggest that TGFβ1 induces the expression of PFKFB4 in A549 and MCF‐10 cells, and PFKFB4 may be required for TGFβ1‐driven phenotypes such as EMT and invasion in these models.
Coexistence of cerebral venous thrombosis and dural arteriovenous fistula in an adolescent: A case report
Authors: Paudyal Man Bahadur , Bhattarai Madhur , Mehta Neha , Gautam Niraj , Baral Bikas , Sharma Niraj Kumar , Basnet Rashika , Ghimire Bardan
Published: September 2023

Key Clinical Message

Clinicians should consider central venous thrombosis (CVT) as a differential diagnosis in young adolescents with persistent headaches. It is essential to assess for concurrent CVT and dural arteriovenous fistula (DAVF), particularly in those with a history of CVT.

Cerebral venous thrombosis (CVT) and dural arteriovenous fistula (DAVF) are uncommon vascular disorders with diverse clinical presentations. The coexistence of CVT and DAVF is a rare but important association that may impact the management and prognosis of affected patients. Prothrombotic conditions generally ranging from acquired to genetic, oral contraceptives, malignancy, puerperium, infection, and head injury are the common risk factors for cerebral venous thrombosis. Here, we present a case of 18 years males who developed recurrent cerebral thrombosis on the background of the presence of an arteriovenous fistula.
Double Hydroamination of Terminal Alkynes over Beta Zeolite‐Supported CuO Catalyst
Authors: Sai Gajula Krishna , Kumar Macharla Arun , Amrutham Vasu , Rohini Yennamaneni Divya , Boosa Murali , Madasu Ramulamma , Kumar Chelukalapally Anil , Nama Narender
Published: September 2023

An effective and novel heterogeneous beta zeolite supported CuO (Cuβ) catalyzed double hydroamination of alkynes for the synthesis of 2,3‐dihydroquinazolin‐4(1H)‐ones has been achieved in a solvent‐free condition. Cuβ zeolite was synthesized by impregnation method. The synthesized Cuβ was characterized by X‐ray diffraction, Fourier transformation infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, and X‐ray photoelectron spectroscopy. Pure Hβ (18 % yield) or Nano CuO (44 % yield) displayed poor catalytic activity, whereas 1 % Cuβ (96 % yield) showed higher catalytic activity for double hydroamination of alkynes due to the synergistic effect of CuO and Hβ zeolite in the reaction. The new protocol offers an appealing approach to a number of di‐substituted quinazolinones with decent to excellent yields. The catalyst has been successfully recycled for up to 5 consecutive cycles.