bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparing the effectiveness of active and reactive mattresses in pressure injury healing: a pilot study
Authors: Rae Katherine E , Barker Judith , Isbel Stephen , Upton Dominic
Published: June 2024

Objective:

A feasibility study to test the proposed methodology for a larger randomised control trial was conducted, investigating the comparative effectiveness of the two types of pressure management support surfaces with regards to healing pressure injuries (PI). A secondary objective was to provide insights into the user acceptability of the two types of pressure management support surfaces.

Method:

A randomised control feasibility study was conducted in a community health setting in Canberra, Australia. Patients aged ≥65 years with an existing Stage 2 PI who slept in a bed were eligible. Participants were randomised to either the active mattress group or the reactive mattress group for use on their bed. All participants received standard wound care by community nursing staff and were provided an air-flotation cushion for use when not in bed. Photographs were taken and used for blind assessment of wound healing. Secondary information was gathered through a survey regarding user acceptability of the support surfaces and changes in habits regarding PI prevention strategies.

Results:

In total, five patients were recruited, with one passing away prior to mattress allocation. Results were inconclusive with regards to comparative effectiveness and user acceptability due to the small sample size; however, secondary data indicated an increasing implementation of PI prevention strategies.

Conclusion:

This study confirmed the need for further high quality research comparing reactive and active pressure mattresses. Trends indicate the importance of including education on PI prevention strategies to promote changes in behaviour. Changes to the proposed methodology will be made to increase recruitment in the primary study.
Fournier's gangrene originating from the perianal region or the urogenital area: does it make a difference?
Authors: Cengiz Fevzi , Gur Emine Ozlem , Atay Yigit Doganay , Kamer Kemal Erdinc , Bozok Yeliz Yilmaz , Bag Halis , Gungor Feyyaz , Haciyanli Mehmet
Published: June 2024

Objective:

Fournier's gangrene (FG) is a rare and serious disorder which is associated with high mortality. In the literature, there is no study evaluating clinician-, patient- and disease-related factors affecting disease outcomes according to aetiological variation in FG. In our study, laboratory results and Uludag Fournier's Gangrene Severity Index (UFGSI) score, clinical characteristics and mortality rates were compared between FG originating from perianal or from urogenital regions.

Method:

Platelet-to-lymphocyte ratio, neutrophil-to-lymphocyte ratio, Laboratory Risk Indicator for Necrotizing Fasciitis (LRINEC) and UFGSI risk scores were calculated in patients with FG at presentation to the emergency department. The patients were assigned to two groups according to FG aetiology.

Results:

It was observed that the number of debridement interventions and the need for colostomy were significantly greater in the perianal FG group, while the need for flap or reconstruction was significantly (p=0.002) higher in the genitourinary FG group. No significant difference was detected in mortality between groups and the difference in aetiology had no significant effect on the results of the neutrophil-to-lymphocyte ratio, LRINEC or UFGSI scores.

Conclusion:

Laboratory results and UFGSI score were helpful in assessing disease severity independently from aetiology. The higher number of debridement interventions to protect anal function in the perianal group and the greater need for reconstructive surgery in the urogenital group were identified as factors that prolonged length of hospital stay.
Optimizing Resource Allocation in M/M/1/N Queues with Feedback, Discouraged Arrivals, and Reneging for Enhanced Service Delivery
Authors: Savita  , Kumar Amit , Shekhar Chandra
Published: June 2024

This article presents a novel computational approach for analyzing M/M/1/N queues with feedback, discouraged arrivals, and reneging, under the first-come, first-served (FCFS) discipline. We calculate explicit transient state probabilities and represent results using symmetric tridiagonal matrix eigenvalues. Through numerical simulations, we validate our method, providing practical insights for optimizing resource allocation. Our study contributes to both theory and application, advancing queueing theory and aiding decision-makers in improving service quality and resource management.
Democracy, “Democracy (Reprise),” and the Asian American Ambivalence of Soft Power
Authors: Galella Donatella
Published: June 2024

Abstract: In Soft Power (2019), David Henry Hwang and Jeanine Tesori deconstruct and demonstrate the affective power of American musicals by reversing The King and I (1951). Soft Power satirizes democracy, white supremacy, and gun violence with whiteface, meta-propaganda, and a sweeping Broadway-style score. In the torch song “Democracy” and its reprise, the artists articulate Asian American ambivalence about US democracy: hope in its promise to lift everyone, despair in its rootedness in racism, and cognizance of musical theatre as a delivery system for ideology. This article uses repetition, interviews, reviews, and dramaturgy to consider Soft Power , soft power, and democracy.
Tunable band-stop fiber filter based on laser-induced graphene metamaterial in THz frequency
Authors: Tian Ziping , Luo Zhenyang , Lv Xianpeng , Xie Manyan , Peng Gangding , Kong Depeng , Lu Huihui , Guan Heyuan
Published: June 2024

As an important device in the application of terahertz (THz) technology, a THz filter has broad application prospects in the fields of THz communication, imaging, and sensing. In this paper, a THz filter based on grating structure laser-induced graphene (LIG)/ side polishing terahertz fiber composite structure is proposed. In the experiment, we achieved the maximum Q factor of 23.83 at the central resonant frequency of 0.715 THz. By modifying the grating structure, a tunable operational span of 269 GHz was achieved, along with a tunable range of 21 GHz through laser stimulation. In testing, we found that LIG materials prepared with circular filling are more sensitive to relatively high-power pump lasers, while LIG samples prepared with line filling exhibit better linear response to laser power. Furthermore, the compact and highly integrated nature of the device suggests its broad potential utility in the realm of THz frequency selection.
Photonic generation of dual-band triangular chirps utilizing a monolithic integrated mutual injection laser and application in radars
Authors: Chen Shilin , Li Jin , Zheng Jilin , Wang Li , Wu Gengze , Zhang Yunshan , Chen Xiangfei , Pu Tao
Published: June 2024

We propose and demonstrate a dual-band microwave photonic radar scheme based on a monolithic integrated mutual injection laser. Based on the photon–photon resonance (PPR) and the gain switching effect of the integrated laser, the C-/X-band triangular chirp signals with high-quality and comparable power at 4.75–5.25 GHz and 9.5–10.5 GHz are generated. In the current proof-of-concept experiment, the range resolution of the dual-band chirp signals can reach 16.9 cm, compared with the single-band chirp signal that cannot distinguish the targets. Through the application of a single integrated device and a transceiver module sharing a set of antennas, the dual-band microwave photonic radar system scheme improves the system integration.
Generating complex vectorial optical fields via surface lattice resonances
Authors: Wu Simeng , Yin Cheng , Yuan Shuaijie , Luo Yinlong , Kan Xuefen , Zhang Yuqiu , Yu Qiang , Wu Jian
Published: June 2024

Vectorial optical fields (VOFs) with extra degrees of freedom hold promise for many photonic applications. However, current methods to generate VOFs are either bulky in size or exhibit limited functionalities. Here, we demonstrate a tunable VOF generator by exciting plasmonic surface lattice resonances (SLRs) with axial symmetry. By meticulously arranging bilayer circular arrays with opposite handedness, we achieve a high Q-factor of 103 via just a few particles despite the general belief that too small array size suppresses the SLRs. This work presents tunable complex VOFs with distinct inhomogeneous spatial polarization distributions, which may enable various applications in integrated and polarization optics.
Simultaneous improvement of the sensitivity and resolution of CPT magnetometers based on phase delay and differential method
Authors: Yuan Jiahao , Du Pengcheng , Yang Feifan , Quan Wei , Li Jin
Published: June 2024

We report a method to enhance the sensitivity of coherent population trapping (CPT) magnetometers using a combination of left-handed and right-handed circularly polarized light phase-delay detection and a differential detection scheme. The approach can achieve a four third-fold enhancement of the CPT dispersion signal slope and a three-fold reduction in noises. The proposed method experimentally exhibits a four third-fold magnetic field resolution enhancement in CPT open-loop measurements, and the differential method could achieve a sensitivity of 1 pT/Hz at 10 Hz and a sensitivity of 0.4 pT/Hz at 50–100 Hz in the CPT closed-loop measurement, which is a four-fold sensitivity enhancement compared to the single-transmitted CPT magnetometer.
Comparative genomics of the closely related fungal genera Cryptococcus and Kwoniella reveals karyotype dynamics and suggests evolutionary mechanisms of pathogenesis
Authors: Coelho Marco A. , David-Palma Márcia , Shea Terrance , Bowers Katharine , McGinley-Smith Sage , Mohammad Arman W. , Gnirke Andreas , Yurkov Andrey M. , Nowrousian Minou , Sun Sheng , Cuomo Christina A. , Heitman Joseph
Published: June 2024

In exploring the evolutionary trajectories of both pathogenesis and karyotype dynamics in fungi, we conducted a large-scale comparative genomic analysis spanning the Cryptococcus genus, encompassing both global human fungal pathogens and nonpathogenic species, and related species from the sister genus Kwoniella. Chromosome-level genome assemblies were generated for multiple species, covering virtually all known diversity within these genera. Although Cryptococcus and Kwoniella have comparable genome sizes (about 19.2 and 22.9 Mb) and similar gene content, hinting at preadaptive pathogenic potential, our analysis found evidence of gene gain (via horizontal gene transfer) and gene loss in pathogenic Cryptococcus species, which might represent evolutionary signatures of pathogenic development. Genome analysis also revealed a significant variation in chromosome number and structure between the 2 genera. By combining synteny analysis and experimental centromere validation, we found that most Cryptococcus species have 14 chromosomes, whereas most Kwoniella species have fewer (11, 8, 5, or even as few as 3). Reduced chromosome number in Kwoniella is associated with formation of giant chromosomes (up to 18 Mb) through repeated chromosome fusion events, each marked by a pericentric inversion and centromere loss. While similar chromosome inversion–fusion patterns were observed in all Kwoniella species with fewer than 14 chromosomes, no such pattern was detected in Cryptococcus. Instead, Cryptococcus species with less than 14 chromosomes showed reductions primarily through rearrangements associated with the loss of repeat-rich centromeres. Additionally, Cryptococcus genomes exhibited frequent interchromosomal translocations, including intercentromeric recombination facilitated by transposons shared between centromeres. Overall, our findings advance our understanding of genetic changes possibly associated with pathogenicity in Cryptococcus and provide a foundation to elucidate mechanisms of centromere loss and chromosome fusion driving distinct karyotypes in closely related fungal species, including prominent global human pathogens.
Common data models to streamline metabolomics processing and annotation, and implementation in a Python pipeline
Authors: Mitchell Joshua M. , Chi Yuanye , Thapa Maheshwor , Pang Zhiqiang , Xia Jianguo , Li Shuzhao
Published: June 2024

To standardize metabolomics data analysis and facilitate future computational developments, it is essential to have a set of well-defined templates for common data structures. Here we describe a collection of data structures involved in metabolomics data processing and illustrate how they are utilized in a full-featured Python-centric pipeline. We demonstrate the performance of the pipeline, and the details in annotation and quality control using large-scale LC-MS metabolomics and lipidomics data and LC-MS/MS data. Multiple previously published datasets are also reanalyzed to showcase its utility in biological data analysis. This pipeline allows users to streamline data processing, quality control, annotation, and standardization in an efficient and transparent manner. This work fills a major gap in the Python ecosystem for computational metabolomics.
Cholinergic modulation supports dynamic switching of resting state networks through selective DMN suppression
Authors: Sanda Pavel , Hlinka Jaroslav , van den Berg Monica , Skoch Antonin , Bazhenov Maxim , Keliris Georgios A. , Krishnan Giri P.
Published: June 2024

Brain activity during the resting state is widely used to examine brain organization, cognition and alterations in disease states. While it is known that neuromodulation and the state of alertness impact resting-state activity, neural mechanisms behind such modulation of resting-state activity are unknown. In this work, we used a computational model to demonstrate that change in excitability and recurrent connections, due to cholinergic modulation, impacts resting-state activity. The results of such modulation in the model match closely with experimental work on direct cholinergic modulation of Default Mode Network (DMN) in rodents. We further extended our study to the human connectome derived from diffusion-weighted MRI. In human resting-state simulations, an increase in cholinergic input resulted in a brain-wide reduction of functional connectivity. Furthermore, selective cholinergic modulation of DMN closely captured experimentally observed transitions between the baseline resting state and states with suppressed DMN fluctuations associated with attention to external tasks. Our study thus provides insight into potential neural mechanisms for the effects of cholinergic neuromodulation on resting-state activity and its dynamics.
Metabolic profiles of children aged 2–5 years born after frozen and fresh embryo transfer: A Chinese cohort study
Authors: Zhou Wei , Feng Wanbing , Chang Jinli , Hu Jingmei , Li Fuxia , Hu Kuona , Jiao Jiejing , Xue Xinyi , Lan Ting , Wan Wenjing , Chen Zi-Jiang , Cui Linlin
Published: June 2024

Background

Frozen embryo transfer (FET) has become a widely employed assisted reproductive technology technique. There have historically been concerns regarding the long-term metabolic safety of FET technology in offspring due to pregnancy-induced hypertension and large for gestational age, both of which are well-recognized factors for metabolic dysfunction of children. Therefore, we aimed to compare the metabolic profiles of children born after frozen versus fresh embryo transfer at 2 to 5 years of age.

Methods and findings

This was a prospective cohort study. Using data from the “Assisted Reproductive Technology borned KIDs (ARTKID),” a birth cohort of offspring born from assisted reproductive technology at the Institute of Women, Children and Reproductive Health, Shandong University, China. We included 4,246 singletons born after FET (n = 2,181) and fresh embryo transfer (n = 2,065) enrolled between 2008 and 2019 and assessed the glucose and lipid variables until the age of 2 to 5 years. During a mean follow-up of 3.6 years, no significant differences were observed in fasting blood glucose, fasting insulin, Homeostatic Model Assessment of Insulin Resistance Index, total cholesterol, triglycerides, low-density lipoprotein-cholesterol, and high-density lipoprotein-cholesterol levels between offspring conceived by fresh and frozen embryo transfer in the crude model and adjusted model (adjusted for parental age, parental body mass index, parental education level, paternal smoking, parity, offspring age and sex). These results remained consistent across subgroup analyses considering offspring age, the stage of embryo transfer, and the mode of fertilization. Results from sensitivity analysis on children matched for age within the cohort remains the same. The main limitation of our study is the young age of the offspring.

Conclusions

In this study, the impact of FET on glucose and lipid profiles during early childhood was comparable to fresh embryo transfer. Long-term studies are needed to evaluate the metabolic health of offspring born after FET.
An integrated analysis of the structural changes and gene expression of spleen in human visceral leishmaniasis with and without HIV coinfection
Authors: Fontes Jonathan L. M. , Khouri Ricardo , Reinaldo Luis Gustavo C. , Hassegawa Erina M. A. , Meneses Filho Antônio José , de Melo Caroline V. B. , Ramos Pablo Ivan P. , de Deus Moura Rafael , Pagliari Carla , Santos Marta , Araújo Raimundo José C. , Van Weyenbergh Johan , de Freitas Luiz A. R. , Costa Carlos Henrique N. , dos-Santos Washington L. C.
Published: June 2024

The spleen plays a pivotal role in the pathogenesis of visceral leishmaniasis. In severe forms of the disease, the spleen undergoes changes that can compromise its function in surveilling blood-circulating pathogens. In this study, we present an integrated analysis of the structural and gene expression alterations in the spleens of three patients with relapsing visceral leishmaniasis, two of whom were coinfected with HIV. Our findings reveal that the IL6 signaling pathway plays a significant role in the disorganization of the white pulp, while BCL10 and ICOSLG are associated with spleen organization. Patients coinfected with HIV and visceral leishmaniasis exhibited lower splenic CD4+ cell density and reduced expression of genes such as IL15. These effects may contribute to a compromised immune response against L. infantum in coinfected individuals, further impacting the structural organization of the spleen.
Acceptability and feasibility of tests for infection, serological testing, and photography to define need for interventions against trachoma
Authors: Renneker Kristen K. , Mtuy Tara B. , Kabona George , Mbwambo Stephen Gabriel , Mosha Patrick , Mollel Jeremiah Mepukori , Hooper PJ , Emerson Paul M. , Hollingsworth T. Deirdre , Butcher Robert , Solomon Anthony W. , Harding-Esch Emma M.
Published: June 2024

Background

Trachoma causes blindness due to repeated conjunctival infection by Chlamydia trachomatis (Ct). Transmission intensity is estimated, for programmatic decision-making, by prevalence of the clinical sign trachomatous inflammation—follicular (TF) in children aged 1–9 years. Research into complementary indicators to field-graded TF includes work on conjunctival photography, tests for ocular Ct infection, and serology. The perceived acceptability and feasibility of these indicators among a variety of stakeholders is unknown.

Methodology

Focus group discussions (FGDs) with community members and in-depth interviews (IDIs) with public health practitioners in Tanzania were conducted. FGDs explored themes including participants’ experience with, and thoughts about, different diagnostic approaches. The framework method for content analysis was used. IDIs yielded lists of perceived strengths of, and barriers to, implementation for programmatic use of each indicator. These were used to form an online quantitative survey on complementary indicators distributed to global stakeholders via meetings, mailing lists, and social media posts.

Results

Sixteen FGDs and 11 IDIs were conducted in October–November 2022. In general, all proposed sample methods were deemed acceptable by community members. Common themes included not wanting undue discomfort and a preference for tests perceived as accurate. Health workers noted the importance of community education for some sample types. The online survey was conducted in April–May 2023 with 98 starting the questionnaire and 81 completing it. Regarding barriers to implementing diagnostics, the highest agreement items related to feasibility, rather than acceptability. No evidence of significant differences was found in responses pertaining to community acceptability based on participant characteristics.

Conclusions

All of the indicators included were generally deemed acceptable by all stakeholders in Tanzania, although community education around the benefits and risks of different sample types, as well as addressing issues around feasibility, will be key to successful, sustainable integration of these indicators into trachoma programs.
High resolution assessment of commercial fisheries activity along the US West Coast using Vessel Monitoring System data with a case study using California groundfish fisheries
Authors: Wang Yi-Hui , Ruttenberg Benjamin I. , Walter Ryan K. , Pendleton Frank , Samhouri Jameal F. , Liu Owen R. , White Crow
Published: June 2024

Commercial fisheries along the US West Coast are important components of local and regional economies. They use various fishing gear, target a high diversity of species, and are highly spatially heterogeneous, making it challenging to generate a synoptic picture of fisheries activity in the region. Still, understanding the spatial and temporal dynamics of US West Coast fisheries is critical to meet the US legal mandate to manage fisheries sustainably and to better coordinate activities among a growing number of users of ocean space, including offshore renewable energy, aquaculture, shipping, and interactions with habitats and key non-fishery species such as seabirds and marine mammals. We analyzed vessel tracking data from Vessel Monitoring System (VMS) from 2010 to 2017 to generate high-resolution spatio-temporal estimates of contemporary fishing effort across a wide range of commercial fisheries along the entire US West Coast. We identified over 247,000 fishing trips across the entire VMS data, covering over 25 different fisheries. We validated the spatial accuracy of our analyses using independent estimates of spatial groundfish fisheries effort generated through the NOAA’s National Marine Fisheries Service Observer Program. Additionally, for commercial groundfish fisheries operating in federal waters in California, we combined the VMS data with landings and ex-vessel value data from California commercial fisheries landings receipts to generate highly resolved estimates of landings and ex-vessel value, matching over 38,000 fish tickets with VMS data that included 87% of the landings and 76% of the ex-vessel value for groundfish. We highlight fisheries-specific and spatially-resolved patterns of effort, landings, and ex-vessel value, a bimodal distribution of fishing effort with respect to depth, and variable and generally declining effort over eight years. The information generated by our study can help inform future sustainable spatial fisheries management and other activities in the marine environment including offshore renewable energy planning.
Synergistic sequential oxidative extraction for nanofibrillated cellulose isolated from oil palm empty fruit bunch
Authors: Abd Manaf Mastura , Harun Shuhaida , Md. Jahim Jamaliah , Sajab Mohd Shaiful , Ibrahim Zulkifli
Published: June 2024

This research presents a comprehensive study of sequential oxidative extraction (SOE) consisting of alkaline and acidic oxidation processes to extract nanocellulose from plant biomass. This proposed process is advantageous as its operation requires a minimum process with mild solvents, and yet successfully isolated high-quality nanofibrillated cellulose (NFC) from raw OPEFB. The SOE involved ammonium hydroxide (NH4OH, 2.6 M) and formic acid (HCOOH, 5.3 M) catalyzed by hydrogen peroxide (H2O2, 3.2 M). This approach was used to efficiently solubilize the lignin and hemicellulose from Oil Palm Empty Fruit Bunch (OPEFB) at the temperature of 100°C and 1 h extraction time, which managed to retain fibrous NFC. The extracted solid and liquor at each stage were studied extensively through physiochemical analysis. The finding indicated that approximately 75.3%dwb of hemicellulose, 68.9%dwb of lignin, and 42.0%dwb of extractive were solubilized in the first SOE cycle, while the second SOE cycle resulted in 92.3%dwb, 99.6%dwb and 99.8%dwb of solubilized hemicellulose, lignin, and extractive/ash, respectively. High-quality NFC (75.52%dwb) was obtained for the final extracted solid with 76.4% crystallinity, which is near the crystallinity of standard commercial NFC. The proposed process possesses an effective synergy in producing NFC from raw OPEFB with less cellulose degradation, and most of the degraded hemicellulose and lignin are solubilized in the liquor.
Unveiling the nexus between rural population aging, technical efficiency, and carbon emissions in Chinese agriculture
Authors: Song Hongen , Jiang Changyi , Sun Zhaoming
Published: June 2024

Introduction

According to the seventh national population census in China, the proportion of people aged 65 and above in the population reached 13.5%. The aging trend is more pronounced in rural areas, indicating that China has entered an aging society. This article focuses on agricultural carbon emissions in the context of aging, studying the impact of rural population aging on agricultural carbon emissions.

Study objectives

Under the background of deepening population aging, let us discuss how to maintain the green and sustainable development of agriculture in China.

Methodology

Fixed effects and mediating effects models are used. Technical efficiency is used as a mediating variable to discuss the relationship between rural population ageing, technical efficiency and agricultural carbon emissions.

Results

This paper adopts the classical carbon emission calculation theory of IPCC to measure agricultural carbon emissions from 2010 to 2019, and China’s plantation carbon emissions show an "inverted U-shaped" trend, reaching a high level in 2015 and then starting to decline. In addition, the fixed-effects benchmark regression found that the aging of the rural population promotes agricultural carbon emissions, and the technical efficiency of agriculture suppresses agricultural carbon emissions. Finally, the mediating effect model is applied to explore the relationship between the three. Using technical efficiency as the mediating variable, it is found that under the masking effect, rural population aging will weaken agricultural carbon emissions through technical efficiency, thus achieving the suppression of agricultural carbon emissions.

Policy recommendation

The formulation and modification of agricultural carbon-reducing policy row policies should take full account of the broader context of rural population ageing; increase the interconnectedness and interaction between rural population ageing and agricultural production technology, and actively play a positive role in promoting the efficiency of agricultural technology as a result of rural population ageing; and, in accordance with the actual situation of agricultural research, appropriately increase the strength of financial support for agriculture to improve agricultural technology and promote low-carbon development in agriculture.
Molecular identification of Coxiella burnetii in raw milk samples collected from farm animals in districts Kasur and Lahore of Punjab, Pakistan
Authors: Shujat Shahpal , Shehzad Wasim , Anjum Aftab Ahmad , Hertl Julia A. , Gröhn Yrjö T. , Zahoor Muhammad Yasir
Published: June 2024

Coxiella burnetii is the worldwide zoonotic infectious agent for Q fever in humans and animals. Farm animals are the main reservoirs of C. burnetii infection, which is mainly transmitted via tick bites. In humans, oral, percutaneous, and respiratory routes are the primary sources of infection transmission. The clinical signs vary from flu-like symptoms to endocarditis for humans’ acute and chronic Q fever. While it is usually asymptomatic in livestock, abortion, stillbirth, infertility, mastitis, and endometritis are its clinical consequences. Infected farm animals shed C. burnetii in birth products, milk, feces, vaginal mucus, and urine. Milk is an important source of infection among foods of animal origin. This study aimed to determine the prevalence and molecular characterization of C. burnetii in milk samples of dairy animals from two districts in Punjab, Pakistan, as it has not been reported there so far. Using a convenience sampling approach, the current study included 304 individual milk samples from different herds of cattle, buffalo, goats, and sheep present on 39 farms in 11 villages in the districts of Kasur and Lahore. PCR targeting the IS1111 gene sequence was used for its detection. Coxiella burnetii DNA was present in 19 of the 304 (6.3%) samples. The distribution was 7.2% and 5.2% in districts Kasur and Lahore, respectively. The results showed the distribution in ruminants as 3.4% in buffalo, 5.6% in cattle, 6.7% in goats, and 10.6% in sheep. From the univariable analysis, the clinical signs of infection i.e. mastitis and abortion were analyzed for the prevalence of Coxiella burnetii. The obtained sequences were identical to the previously reported sequence of a local strain in district Lahore, Sahiwal and Attock. These findings demonstrated that the prevalence of C. burnetii in raw milk samples deserves more attention from the health care system and veterinary organizations in Kasur and Lahore of Punjab, Pakistan. Future studies should include different districts and human populations, especially professionals working with animals, to estimate the prevalence of C. burnetii.
Differences in condom access and use and associated factors between persons with and without disabilities receiving social cash transfers in Luapula province, Zambia—A cross-sectional study
Authors: Chipanta David , Estill Janne , Stöckl Heidi , Toska Elona , Chanda Patrick , Mwanza Jason , Kaila Kelly , Matome Chisangu , Tembo Gelson , Keiser Olivia
Published: June 2024

Persons with disabilities are disadvantaged in accessing sexual and reproductive health services, including condoms. In this study, we investigated whether condom access and use and their associated factors differed between persons with and without disabilities. We used data from adults in households receiving the Government of Zambia social cash transfers (SCT) in four districts of Luapula province. Condom access and use was the outcome. Disability, defined by the Washington Group Short Set Questions on Disability, was the main predictor. We performed logistic regression analyses to determine the associations between condom access and use and disability. In multivariable analyses, we controlled for covariates including age, sex, marital status, poverty status, HIV testing, and receiving the SCT. The sample comprised 1,143 people aged 16–49, with a median age of 21 years (interquartile range 18–28); 57.4% (n = 656) were female, 86.5% (n = 989) accessed and used condoms, and 17.9% (n = 205) were disabled, rating themselves with a 3 or a 4 on a scale of 1 = “not limited” to 4 = “cannot at all” in performing any of the six daily functions (seeing, hearing, walking, cognition, self-care, or communicating). Nearly sixty percent(58.5% (n = 120)) of persons with disabilities were female, 79.5% (n = 163) reported being very poor, 87.8% (n = 180) reported receiving SCT, and 86.3% (n = 177) reported accessing and using condoms. Condom access and use did not differ between persons with and without disabilities (adjusted odds ratio: 1.09; 95% confidence interval [CI]: 0.60–1.98]). We found no differences between persons with and without disabilities in condom access and use. We established that individual-level factors such as age, sex, marital status, and knowledge of being HIV positive might play a more important role in condom access and use than disability. Condom promotion interventions should account for these factors.
Factors influencing the efficacy of recombinant tissue plasminogen activator: Implications for ischemic stroke treatment
Authors: Víteček Jan , Vítečková Wünschová Andrea , Thalerová Sandra , Gulati Sumeet , Kubala Lukáš , Capandová Michaela , Hampl Aleš , Robert Mikulík
Published: June 2024

Intravenous thrombolysis with a recombinant tissue plasminogen activator (rt-PA) is the first-line treatment of acute ischemic stroke. However, successful recanalization is relatively low and the underlying processes are not completely understood. The goal was to provide insights into clinically important factors potentially limiting rt-PA efficacy such as clot size, rt-PA concentration, clot age and also rt-PA in combination with heparin anticoagulant. We established a static in vitro thrombolytic model based on red blood cell (RBC) dominant clots prepared using spontaneous clotting from the blood of healthy donors. Thrombolysis was determined by clot mass loss and by RBC release. The rt-PA became increasingly less efficient for clots larger than 50 μl at a clinically relevant concentration of 1.3 mg/l. A tenfold decrease or increase in concentration induced only a 2-fold decrease or increase in clot degradation. Clot age did not affect rt-PA-induced thrombolysis but 2-hours-old clots were degraded more readily due to higher activity of spontaneous thrombolysis, as compared to 5-hours-old clots. Finally, heparin (50 and 100 IU/ml) did not influence the rt-PA-induced thrombolysis. Our study provided in vitro evidence for a clot size threshold: clots larger than 50 μl are hard to degrade by rt-PA. Increasing rt-PA concentration provided limited thrombolytic efficacy improvement, whereas heparin addition had no effect. However, the higher susceptibility of younger clots to thrombolysis may prompt a shortened time from the onset of stroke to rt-PA treatment.
Time to death and predictors of mortality among early neonates admitted to neonatal intensive care unit of Addis Ababa public Hospitals, Ethiopia: Institutional-based prospective cohort study
Authors: Malka Erean Shigign , Solomon Tarekegn , Kassa Dejene Hailu , Erega Besfat Berihun , Tufa Derara Girma
Published: June 2024

Introduction

The largest risk of child mortality occurs within the first week after birth. Early neonatal mortality remains a global public health concern, especially in sub-Saharan African countries. More than 75% of neonatal death occurs within the first seven days of birth, but there are limited prospective follow- up studies to determine time to death, incidence and predictors of death in Ethiopia particularly in the study area. The study aimed to determine incidence and predictors of early neonatal mortality among neonates admitted to the neonatal intensive care unit of Addis Ababa public hospitals, Ethiopia 2021.

Methods

Institutional prospective cohort study was conducted in four public hospitals found in Addis Ababa City, Ethiopia from June 7th, 2021 to July 13th, 2021. All early neonates consecutively admitted to the corresponding neonatal intensive care unit of selected hospitals were included in the study and followed until 7 days-old. Data were coded, cleaned, edited, and entered into Epi data version 3.1 and then exported to STATA software version 14.0 for analysis. The Kaplan Meier survival curve with log- rank test was used to compare survival time between groups. Moreover, both bi-variable and multivariable Cox proportional hazard regression model was used to identify the predictors of early neonatal mortality. All variables having P-value ≤0.2 in the bi-variable analysis model were further fitted to the multivariable model. The assumption of the model was checked graphically and using a global test. The goodness of fit of the model was performed using the Cox-Snell residual test and it was adequate.

Results

A total of 391 early neonates with their mothers were involved in this study. The incidence rate among admitted early neonates was 33.25 per 1000 neonate day’s observation [95% confidence interval (CI): 26.22, 42.17]. Being preterm birth [adjusted hazard ratio (AHR): 6.0 (95% CI 2.02, 17.50)], having low fifth minute Apgar score [AHR: 3.93 (95% CI; 1.5, 6.77)], low temperatures [AHR: 2.67 (95%CI; 1.41, 5.02)] and, resuscitating of early neonate [AHR: 2.80 (95% CI; 1.51,5.10)] were associated with increased hazard of early neonatal death. However, early neonatal crying at birth [AHR: 0.48 (95%CI; 0.26, 0.87)] was associated with reduced hazard of death.

Conclusions

Early neonatal mortality is high in Addis Ababa public Hospitals. Preterm birth, low five-minute Apgar score, hypothermia and crying at birth were found to be independent predictors of early neonatal death. Good care and attention to neonate with low Apgar scores, premature, and hypothermic neonates.
Genome-wide identification and characterization of DTX family genes highlighting their locations, functions, and regulatory factors in banana (Musa acuminata)
Authors: Amin Al , Naim Md. Darun , Islam Nurul , Mollah Md. Nurul Haque
Published: June 2024

The detoxification efflux carriers (DTX) are a significant group of multidrug efflux transporter family members that play diverse functions in all kingdoms of living organisms. However, genome-wide identification and characterization of DTX family transporters have not yet been performed in banana, despite its importance as an economic fruit plant. Therefore, a detailed genome-wide analysis of DTX family transporters in banana (Musa acuminata) was conducted using integrated bioinformatics and systems biology approaches. In this study, a total of 37 DTX transporters were identified in the banana genome and divided into four groups (I, II, III, and IV) based on phylogenetic analysis. The gene structures, as well as their proteins’ domains and motifs, were found to be significantly conserved. Gene ontology (GO) annotation revealed that the predicted DTX genes might play a vital role in protecting cells and membrane-bound organelles through detoxification mechanisms and the removal of drug molecules from banana cells. Gene regulatory analyses identified key transcription factors (TFs), cis-acting elements, and post-transcriptional regulators (miRNAs) of DTX genes, suggesting their potential roles in banana. Furthermore, the changes in gene expression levels due to pathogenic infections and non-living factor indicate that banana DTX genes play a role in responses to both biotic and abiotic stresses. The results of this study could serve as valuable tools to improve banana quality by protecting them from a range of environmental stresses.
Highly smooth and parameter independent obstacle avoidance method for autonomous vehicle with velocity-varying obstacle
Authors: Yi Nanxi , Liu Zhixian , Yuan Xiaofang
Published: June 2024

One of the primary challenges for autonomous vehicle (AV) is planning a collision-free path in dynamic environment. It is a tricky task for achieving high-performance obstacle avoidance with velocity-varying obstacle. To solve this problem, a highly smooth and parameter independent obstacle avoidance method for autonomous vehicle with velocity-varying obstacle (HSPI-OAM) is presented in this work. The proposed method uses the virtual collision point model to accurately design the desired acceleration, which makes the obtained path highly smooth. At the same time, the method gets rid of the dependence on parameter adjustment and has strong adaptability to different environments. The simulation is implemented on the Matlab-Carsim co-simulation platform, and the simulation results show that the path planned by HSPI-OAM has good performance for obstacle with acceleration.
Lifestyle practices and associated factors among adults with hypertension: Conquering Hypertension in Vietnam-solutions at the grassroots level study
Authors: Nguyen Phuong H. , Goldberg Robert J. , Allison Jeroan J. , Nguyen Diep B. , Tran Ha T. , Tran Oanh M. , Ha Duc A. , Nguyen Hieu L. , Tran Brittany A. , Wang Bo , Nguyen Hoa L.
Published: June 2024

Background

Vietnam is experiencing an increasing prevalence of hypertension in its adult population. In addition to medical therapy, modifying adverse lifestyle practices is important for effective blood pressure control. There are limited data on unhealthy lifestyle practices in patients with chronic diseases, however, particularly among hypertensive patients living in rural Vietnam. Our study objectives were to examine the prevalence of unhealthy lifestyle practices and associated factors among rural Vietnamese adults with uncontrolled hypertension.

Methods

Data from the baseline survey of a cluster randomized trial among hypertensive Vietnamese adults (2017–2022) were utilized. Information on unhealthy lifestyle practices including smoking, excessive alcohol consumption, physical inactivity, and inadequate fruit and vegetable intake was collected from study participants. The primary study outcome was having ≥2 unhealthy lifestyle practices. A multivariable logistic regression model was used to examine factors associated with the primary study outcome.

Results

The mean age of the 671 patients was 67 years and 45.0% were men. Nearly three out of every four participants had one or fewer unhealthy practices, 24.0% had two, and 3.3% had three or all four unhealthy lifestyle practices. Men, individuals who did unpaid work or were unemployed, and individuals with hypertension level III were more likely to have ≥2 unhealthy lifestyle practices, whereas individuals with higher education were less likely to have ≥2 unhealthy lifestyle practices compared with respective comparison groups.

Conclusions

We observed a high prevalence of unhealthy lifestyle practices among rural Vietnamese patients with uncontrolled hypertension. Several demographic factors were associated with a greater number of unhealthy lifestyle practices. Newer interventions and educational programs encouraging lifestyle modification practices are needed to control hypertension among adults living in rural settings of Vietnam.
Potential for high dynamic range Sedia Limiting Antigen antibody assay to support viral load monitoring during antiretroviral therapy
Authors: Sempa Joseph B. , Welte Alex
Published: June 2024

Introduction

Viral Load (VL) monitoring is a crucial component of patient care during antiretroviral therapy (ART) but is not routinely available in many resource-constrained settings, where millions of patients will require care for decades to come. We hypothesise a serologic ‘recent infection’ test (Sedia LAg assay) which has a high dynamic range for detecting antigen-driven antibody response can provide informative proxies for VL trajectories.

Methods

A retrospective study where we analysed data linked via specimens in a well-described repository for recent infection test benchmarking (CEPHIA collaboration). Patient panels were comprised of 1) observations straddling ART start; 2) observations from a period of stable viral suppression; 3) observations straddling rebound after a period of viral suppression. We analysed an individual’s Sedia LAg ELISA normalised optical density (ODn) trends within these categories. Using groups 2) and 3) we evaluated the specificity and sensitivity of a proposed proxy for “the latest observation is at a time of VL rebound”; proxy was defined as follows: we estimated patient-specific mean-previous-ODn for all observations with at least two preceding virally suppressed observations. We considered various thresholds to define both “VL suppression” and “ODn uptick”.

Results

In regression analysis by category: 1) ODn gradients are statistically significantly negative just after ART-start (p = 0.010); 2) During periods of stable viral suppression, ODn tended to decline, but not statistically significantly, for a range of clinically meaningful “VL suppression” thresholds; 3) comparing ODn values just before, versus at, “VL rebound”, ODn changes were statistically significantly increasing at rebound (p = 0.001). In the analysis comparing groups 2) and 3), at a Z score threshold of 0.8, the proposed proxy for a first viral rebound had an observed specificity and sensitivity both close to 90%.

Conclusion

The high dynamic range of serological tests previously investigated for defining ‘recent infection’ has potential, as demonstrated using the Sedia LAg ELISA, to provide meaningful information about the success of ART, during treatment initiation, at times of stable suppression, and to flag possible viral rebound. It should be investigated how this can be combined with patient management workflows and (clinical and) other data, to provide efficiencies in long-term monitoring viral control in resource-limited settings.
Strength of female mate preferences in temperature manipulation study supports the signal reliability hypothesis
Authors: Cobb Nicole E. , Mason Samantha M. , Tompkins Keith , Fitschen-Brown Meredith , Rios-Cardenas Oscar , Morris Molly R.
Published: June 2024

Both sexually selected traits and mate preferences for these traits can be context dependent, yet how variation in preferred traits could select for context dependent preferences has rarely been examined. The signal reliability hypothesis predicts that mate preferences vary across contexts (e.g., environments) in relation to the reliability of the information preferred traits provide in those contexts. Extensive variation in copy number of mc4r B alleles on the Y-chromosome that associates with male size in Xiphophorus multilineatus allowed us to use a split-sibling design to determine if male size is more likely to provide information about male genotype (i.e., dam) when males were reared in a warm as compared to a cold environment. We then examined strength of preference for male size by females reared in the same two environments. We found that males were larger in the cold environment, but male size was more variable across dams in the warm environment, and therefore male size would be a more reliable indicator of dam (i.e., genetics) in the warm environment. Females reared in the warm environment had stronger mate preferences based on male size than cold reared females, with a significant influence of dam on strength of preference. Therefore, strength of female preference for male size was influenced by the temperature in which they were reared, with the direction of the difference across treatments supporting the signal reliability hypothesis. Understanding how the reliability of male traits can select for contextual variation in the strength of the female mate preferences will further our discovery of adaptive mate preferences. For example, a relationship between the strength of a female’s mate preference and their growth rates was detected in the context where females had a preference based on male size, supporting a hypothesis from previous work with this species of disassortative mating in relation to growth rates to mitigate a documented growth-mortality tradeoff.
A heuristic method for solving the Steiner tree problem in graphs using network centralities
Authors: Fujita Misa , Shimada Yutaka , Kimura Takayuki , Ikeguchi Tohru
Published: June 2024

We propose a heuristic method of using network centralities for constructing small-weight Steiner trees in this paper. The Steiner tree problem in graphs is one of the practical NP-hard combinatorial optimization problems. Given a graph and a set of vertices called terminals in the graph, the objective of the Steiner tree problem in graphs is to find a minimum weight Steiner tree that is a tree containing all the terminals. Conventional construction methods make a Steiner tree based on the shortest paths between terminals. If these shortest paths are overlapped as much as possible, we can obtain a small-weight Steiner tree. Therefore, we proposed to use network centralities to distinguish which edges should be included to make a small-weight Steiner tree. Experimental results revealed that using the vertex or the edge betweenness centralities contributes to making small-weight Steiner trees.