bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of changes of cardiac morphology and function in fetuses with ductus arteriosus constriction by Speckle-tracking echocardiography
Authors: Li Tianjing , Han Jiancheng , Han Yanli , Liu Xiaowei , Gu Xiaoyan , Zhang Ye , Sun Lin , Zhao Ying , Gao Shuang , Hao Xiuxiu , He Yihua
Published: February 2023

Background

Premature ductus arteriosus constriction (DA Con) can result in right ventricular enlargement, right ventricular hypertrophy, and tricuspid regurgitation.

Method

This study retrospectively analyzed 34 singleton fetuses that underwent fetal echocardiography with a diagnosis of DA Con (16 cases with mild to moderate, and 18 cases with moderate to severe) and 45 healthy fetuses. The morphology and function parameters of cardiac, as well as the 24-Segment of ventricles, were compared between the DA Con group and controls, and between the mild to moderate and moderate to severe groups, using the fetal heart quantification (FHQ) technology.

Results

There were no significant difference in left ventricular parameters in DA Con group when compared to controls. Moreover, fetal 4CV-GSI was significantly reduced, as well as the sphericity index (SI), fractional shortening (FS), global longitudinal strain (GS) and fractional area change (FAC) of right ventricle, especially in the basal-middle segments. Compared with the mild to moderate group, LV-FS increased and RV-FS decreased in moderate to severe group.

Conclusion

The results showed that the fetal heart in the DA Con group was different from the controls in morphology and function. FHQ technology provides a comprehensive assessment for the evaluation of cardiac morphological and functional changes in DA Con fetuses.
Delivery and neonatal outcomes of pregnant women during the Shanghai lockdown: A retrospective analysis
Authors: Zhou Fang-Yue , Li Cheng , Qin Kai-Zhou , Luo Chuan , Huang He-Feng , Wu Yan-Ting
Published: February 2023

Objectives

Shanghai witnessed an unprecedented outbreak of COVID-19 and experienced a strict lockdown from March 28, 2022 to May 31, 2022. Most studies to date are on the first lockdown after the outbreak in December 2019. This study aimed to examine the impact of lockdown on delivery and neonatal outcomes among uninfected pregnant women in the new phase of the COVID-19 outbreak.

Methods

A retrospective analysis was conducted in the Obstetrics and Gynecology Hospital of Fudan University. Pregnant women without COVID-19 who delivered from March 28, 2022 to May 31, 2022 (lockdown group) and the same period in 2021 (non-lockdown group) were recruited for this study. Logistic regression models and 1 : 1 propensity score matching (PSM) were used to assess the effect of lockdown on delivery outcomes.

Results

A total of 2,962 patients were included in this study, 1,339 of whom were from the lockdown group. Compared with the non-lockdown group, pregnant women giving birth during lockdown had an increased risk of term prelabor rupture of membranes (TPROM) (aOR = 1.253, 95% CI: 1.026–1.530), and decreased risks of postpartum hemorrhage (PPH) (aOR = 0.362, 95% CI: 0.216–0.606) and fetal malformation (aOR = 0.309, 95% CI: 0.164–0.582). The risk of large for gestational age (LGA) (aOR = 0.802, 95% CI: 0.648–0.992) and rate of admission to the neonatal intensive care unit (NICU) (aOR = 0.722, 95% CI: 0.589–0.885) also significantly declined. After 1 : 1 PSM, the impact of lockdown on the risk of TPROM (aOR = 1.501, 95% CI: 1.083–2.080), PPH (aOR = 0.371, 95% CI: 0.211–0.654), fetal malformation (aOR = 0.332, 95% CI: 0.161–0.684), LGA (aOR = 0.749, 95% CI: 0.594–0.945) and rate of admission to the NICU (aOR = 0.700, 95% CI: 0.564–0.869) all remained. There were no other delivery or neonatal outcomes affected by the lockdown after the COVID-19 outbreak.

Conclusion

This study indicated a significant increase in the risk of term PROM, significant decreases in the risk of PPH, fetal malformation and LGA, and a marked decline in the rate of admission to the NICU during Shanghai Lockdown.
Corneal wound healing and nerve regeneration by novel ophthalmic formulations based on cross-linked sodium hyaluronate, taurine, vitamin B6, and vitamin B12
Authors: Bucolo Claudio , Maugeri Grazia , Giunta Salvatore , D’Agata Velia , Drago Filippo , Romano Giovanni Luca
Published: February 2023

Introduction: To evaluate the pharmacological profile of ocular formulations based on cross-linked sodium hyaluronate (CL-SH), taurine (Tau), vitamin B6 (Vit B6) and vitamin B12 (Vit B12) using in vitro and in vivo paradigms.

Methods: Rabbit corneal epithelial cells were used to assess wound healing and reactive oxygen species (ROS) formation by scratch assay and oxidative stress (0.3 mM H2O2; 30 min), respectively with or without ocular formulations exposure. In vivo studies were carried out on albino rabbits to evaluate corneal nerve regeneration and corneal wound healing with or without treatment with six different formulations. Animals were anesthetized, the corneal epithelium was removed, and formulations were topically administered (30 μL/eye; 3 times/day for 6 days). Slit-lamp observation was carried out at different time points. After 6 days the animals were killed, and corneas were collected to evaluate corneal re-innervation by immunohistochemistry of selective neuronal marker β-III tubulin.

Results: Formulations containing the concentrations 0.16% or 0.32% of cross-linked sodium hyaluronate, taurine, vitamin B6 and vitamin B12 accelerated corneal wound healing. Cells exposed to H2O2 led to significant (p < 0.05) increase of reactive oxygen species concentration that was significantly (p < 0.05) counteract by formulations containing cross-linked sodium hyaluronate (0.32%) and taurine with or without vitamins. The extent of re-innervation, in terms of β-III tubulin staining, was 5-fold greater (p < 0.01) in the eye of rabbits treated with formulation containing 0.32% cross-linked sodium hyaluronate, taurine, vitamins (RenerviX®) compared with the control group (no treatment). Furthermore, re-innervation elicited by RenerviX® was significantly greater (p < 0.01) compared with the group treated with the formulation containing 0.32% cross-linked sodium hyaluronate and taurine without vitamins, and with the group treated with the formulation containing 0.5% linear sodium hyaluronate (SH), taurine, and vitamin B12, respectively.

Discussion: In conclusion, among the formulations tested, the new ophthalmic gel RenerviX® was able to contrast oxidative stress, to accelerate corneal re-epithelization and to promote nerve regeneration.
Impact of polymorphisms in CYP and UGT enzymes and ABC and SLCO1B1 transporters on the pharmacokinetics and safety of desvenlafaxine
Authors: Calleja Sofía , Zubiaur Pablo , Ochoa Dolores , Villapalos-García Gonzalo , Mejia-Abril Gina , Soria-Chacartegui Paula , Navares-Gómez Marcos , de Miguel Alejandro , Román Manuel , Martín-Vílchez Samuel , Abad-Santos Francisco
Published: February 2023

Venlafaxine pharmacokinetic variability and pharmacotherapy outcomes are well known to be related to CYP2D6 pharmacogenetic phenotype. In contrast, scarce pharmacogenetic information is available nowadays concerning desvenlafaxine, its active metabolite first marketed in 2012. The aim of this study was to evaluate the impact of 29 alleles in 12 candidate genes (e.g., CYP enzymes like CYP2D6, CYP3A4, or CYP2C19; ABC transporters like ABCB1; SLCO1B1; and UGT enzymes like UGT1A1) on desvenlafaxine pharmacokinetic variability and tolerability. Pharmacokinetic parameters and adverse drug reaction (ADR) incidence obtained from six bioequivalence clinical trials (n = 98) evaluating desvenlafaxine formulations (five with single dose administration and one with multiple-dose administration) were analyzed. No genetic polymorphism was related to pharmacokinetic variability or ADR incidence. Volunteers enrolled in the multiple-dose clinical trial also showed a higher incidence of ADRs, e.g., xerostomia or appetite disorders. Volunteers experiencing any ADR showed a significantly higher area under the time-concentration curve (AUC) than those not experiencing any ADR (5115.35 vs. 4279.04 ng*h/mL, respectively, p = 0.034). In conclusion, the strong dose-dependent relationship with the occurrence of ADRs confirms that the mechanism of action of desvenlafaxine is essentially dose-dependent.
Device-evaluated autonomic nervous function for predicting ventricular arrhythmias and all-cause mortality in patients who underwent cardiac resynchronization therapy-defibrillator
Authors: Cheng Chendi , Jiang Jiang , Chen Keping , Hua Wei , Su Yangang , Xu Wei , Fan Xiaohan , Dai Yan , Zhang Shu
Published: February 2023

Introduction: Autonomic nervous system (ANS) function quantified by heart rate variability (HRV) was associated with long-term prognosis, but it was rarely used in the evaluation of patients with heart failure, especially those with cardiac resynchronization therapy-defibrillator (CRT-D) implantation. This study aimed to describe the changes in ANS function among patients who underwent CRT-D with remote home monitoring function, and explore predictive value of HRV for ventricular tachyarrhythmias (VTAs) and all-cause mortality.

Method: Patients who underwent CRT-D implantation were included. Device-measured all-day HR, night-time HR, and HRV (measured by the standard deviation of the atrial–atrial sensed intervals) were used to quantify ANS function. Multivariate Cox proportional hazards models were fitted to calculate hazard ratios (HRs) and 95% confidence intervals (CIs) of VTAs or all-cause mortality in relation to ANS function at baseline and 6 months post-implantation. The cutoff value was determined using restrictive cubic splines. Multivariable logistic regression was further established to determine factors influencing postoperative HRV.

Results: A total of 170 patients treated with CRT-D were eligible for analysis. During a median follow-up period of 50.8 months, 61 patients died and 69 patients experienced at least one spontaneous episode of VTAs. At 6 months after CRT implantation, 114 patients showed improvement in HRV, increasing from 66.4 ± 19.4 ms to 76.7 ± 21.2 ms. The postoperative HRV was associated with both all-cause mortality (HRs: 0.983; 95% CI: 0.968 to 0.998, p = 0.012) and VTAs (HRs: 0.973; 95% CI: 0.954 to 0.993, p = 0.008), and the relative risk would significantly increase when the postoperative HRV lower than 75 ms. After adjusting for basic ANS function and possible influencing factors, patients without diabetes (p = 0.018) and with higher daily physical activity (p = 0.041) could maintain higher postoperative HRV after CRT implantation.

Conclusion: More than two-thirds of heart failure patients showed improvement in ANS function following CRT treatment. However, patients with diabetes and low daily physical activity levels have difficulty maintaining a higher postoperative HRV, which is associated with a worse clinical outcome.
Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat
Authors: Schierenbeck Matías , Alqudah Ahmad M. , Thabet Samar G. , Lohwasser Ulrike , Simón María Rosa , Börner Andreas
Published: February 2023

Drought is a major constraint in wheat (Triticum aestivum L.) grain yield. The present work aimed to identify quantitative trait nucleotides (QTNs)/ candidate genes influencing drought tolerance-related traits at the seedling stage in 261 accessions of a diverse winter wheat panel. Seeds from three consecutive years were exposed to polyethylene glycol 12% (PEG-6000) and a control treatment (distilled water). The Farm-CPU method was used for the association analysis with 17,093 polymorphic SNPs. PEG treatment reduced shoot length (SL) (-36.3%) and root length (RL) (-11.3%) compared with control treatments, while the coleoptile length (CL) was increased by 11% under drought conditions, suggesting that it might be considered as an indicator of stress-tolerance. Interestingly, we revealed 70 stable QTN across 17 chromosomes. Eight QTNs related to more than one trait were detected on chromosomes 1B, 2A (2), 2B, 2D, 4B, 7A, and 7B and located nearby or inside candidate genes within the linkage disequilibrium (LD) interval. For instance, the QTN on chromosome 2D is located inside the gene TraesCS2D02G133900 that controls the variation of CL_S and SL_C. The allelic variation at the candidate genes showed significant influence on the associated traits, demonstrating their role in controlling the natural variation of multi-traits of drought stress tolerance. The gene expression of these candidate genes under different stress conditions validates their biological role in stress tolerance. Our findings offer insight into understanding the genetic factors and diverse mechanisms in response to water shortage conditions that are important for wheat improvement and adaptation at early developmental stages.
Genome-wide identification of Mg2+ transporters and functional characteristics of DlMGT1 in Dimocarpus longan
Authors: Lv Xinmin , Huang Shilian , Wang Jing , Han Dongmei , Li Jianguang , Guo Dongliang , Zhu Haifeng
Published: February 2023

Longan (Dimocarpus Longan) is one of the most important fruit crops in Southern China. Lack of available Mg in acidic soil conditions is a limitation to further increasing longan yield. Magnesium transporter (MGT/MRS2) mediates the uptake, transport, and redistribution of Mg2+ in higher plants. To understand the role of MGTs family members in longan Mg deficiency. We identified and analyzed the protein characteristics, phylogeny, expression changes, subcellular localization, and transcriptional regulation of DlMGTs members. The results showed that, twelve DlMGTs are localized in the cell membrane, chloroplast, and nucleus. The evolutionary differences in MGTs between herbaceous and woody species in different plants. The DlMGTs promoters contained many cis-acting elements and transcription factor binding sites related to the hormone, environmental, and stress response. Subcellular localization assays showed that DlMGT1 localizes in the cell membrane of Arabidopsis protoplasts. The candidate transcription factor DlGATA16, which may regulate the expression of DlMGT1, was localized in the nucleus of tobacco leaves. Dual luciferase analysis demonstrated that DlGATA16 is a potential factor regulating the transcriptional activity of DlMGT1. In this study, we identified and analyzed DlMGTs on a genome-wide scale and the subcellular localization and interaction of DlMGT1 and DlGATA16, which has important implications for further functional analysis studies of MGTs and the use of MGT for longan genetic improvement.
The complete chloroplast genome of Hibiscus syriacus using long-read sequencing: Comparative analysis to examine the evolution of the tribe Hibisceae
Authors: Koo Hyunjin , Shin Ah-Young , Hong Seongmin , Kim Yong-Min
Published: February 2023

Hibiscus syriacus, a member of the tribe Hibisceae, is considered an important ornamental and medicinal plant in east Asian countries. Here, we sequenced and assembled the complete chloroplast genome of H. syriacus var. Baekdansim using the PacBio long-read sequencing platform. A quadripartite structure with 161,026 base pairs was obtained, consisting of a pair of inverted repeats (IRA and IRB) with 25,745 base pairs, separated by a large single-copy region of 89,705 base pairs and a short single-copy region of 19,831 base pairs. This chloroplast genome had 79 protein-coding genes, 30 transfer RNA genes, 4 ribosomal RNA genes, and 109 simple sequence repeat regions. Among them, ndhD and rpoC1, containing traces of RNA-editing events associated with adaptive evolution, were identified by analysis of putative RNA-editing sites. Codon usage analysis revealed a preference for A/U-terminated codons. Furthermore, the codon usage pattern had a clustering tendency similar to that of the phylogenetic analysis of the tribe Hibisceae. This study provides clues for understanding the relationships and refining the taxonomy of the tribe Hibisceae.
Assessing risk from invasive alien plants in China: Reconstructing invasion history and estimating distribution patterns of Lolium temulentum and Aegilops tauschii
Authors: Yang Ming , Zhao Haoxiang , Xian Xiaoqing , Wang Rui , Yang Nianwan , Chen Li , Liu Wan-xue
Published: February 2023

Introduction

The establishment of invasive alien plants (IAPs) is primarily driven by climate warming and human activities, and their populations have a negative impact on agricultural economics, ecological systems, and human health.Lolium temulentumandAegilops tauschiiare critical IAPs in China because they reduce the quality of cereal grains and decrease wheat yields.Lolium temulentumis a winter-temperate weed that spreads easily and is poisonous to humans and animals.Aegilops tauschiiis resistant to herbicides, has a high reproductive rate, and frequently grows in wheat. Both species have been listed in the Ministry of Agriculture and Rural Affairs of the People’s Republic of China’s management catalog since 2006.

Methods

In the present study, the historical occurrence and invasion of each species were collected and reconstructed, which showed that the population outbreak ofL. temulentumbegan in 2010, whereas that ofA. tauschiibegan in 2000. Using the optimal MaxEnt model, the geographical distributions ofL. temulentumandA. tauschiiwere predicted based on screened species occurrences and environmental variables under the current and three future scenarios in the 2030s and 2050s (i.e., SSP1-2.6, SSP2-4.5, and SSP5-8.5).

Results

The mean AUC values were 0.867 and 0.931 forL. temulentumandA. tauschii, respectively. Human influence index (HII), mean temperature of coldest quarter (bio11), and precipitation of coldest quarter (bio19) were the most significant variables forL. temulentum, whereas human influence index, temperature seasonality (standard deviation×100) (bio4), and annual mean temperature (bio1) were the critical environmental variables forA. tauschi.Suitable habitat areas in China forL. temulentumandA. tauschiicurrently covered total areas of 125 × 104and 235 × 104km2, respectively. Future suitable areas ofL. temulentumreached the maximum under SSP2-4.5, from 2021 to 2060, whereas forA. tauschiithey reached the maximum under SSP5-8.5, from 2021 to 2060. Furthermore, the overlap area under the current climate conditions forL. temulentumandA. tauschiiwas approximately 90 × 104km2, mainly located in Hubei, Anhui, Jiangsu, Shandong, Henan, Shaanxi, Shanxi, and Hebei. The overlap areas decreased in the 2030s, increased in the 2050s, and reached a maximum under SSP1-2.6 (or SSP2-4.5) with an approximate area of 104 × 104km2. The centroid ofL. temulentumin Henan was transferred to the southwest, whereas forA. tauschiiit transferred to higher latitudes in the northeast.

Discussion

Our findings provide a practical reference for the early warning, control, and management of these two destructive IAP populations in China.
Promoting prosocial behavior in an unequal world
Authors: Kirkland Kelly , Jetten Jolanda , Wilks Matti , Kirby James
Published: February 2023

Amid a global pandemic and the climate crisis, there is an increasing need to understand how to promote largescale, coordinated action between different groups. Yet certain factors such as inequality can hinder cooperation. We aimed to establish how to orient groups toward a superordinate goal when they have unequal resources. Participants were divided into two ‘countries’ and asked to assemble LEGO bricks into food (by building pieces in a certain order) to prevent starvation among ‘the people’. One ‘country’ had few LEGO bricks whereas the other had an abundance, and the only way to maximize food creation was for the groups to work together. We assessed the efficacy of three diverse interventions on superordinate behavior and attitudes: compassion meditation training (Study 1), lower inequality (Study 2), and the introduction of a pro-sharing group norm by a confederate (Study 3). Compassion meditation training and altering the degree of inequality between groups did not have a clear effect on collaborative action. Only the introduction of a pro-sharing group norm enhanced sharing behavior, made participants feel more cooperative and reduced fears of being compassionate toward others. Our findings speak to the importance of leadership in promoting coordinated action to address challenges that face the superordinate group.
Developing local crisis leadership – A research and training agenda
Authors: Eid Jarle , Hansen Anita Lill , Andreassen Natalia , Espevik Roar , Brattebø Guttorm , Johnsen Bjørn Helge
Published: February 2023

The crisis triggered by Covid-19 has exposed the interdependencies of modern society and sparked interest in local response to protracted and complex crisis situations. There has been a growing awareness and interest in the key roles of political and professional stakeholders, their emotional regulation and how they influence team performance and outcomes in dealing with uncertainty and complex crisis situations. While cognitive and behavioral aspects of crisis leadership are well researched, less is understood about how one can mitigate negative emotions, instill trust, or restore public faith and support of security forces and emergency response teams during crises. In addressing this gap, we propose a simplified conceptual roadmap for research and training of local crisis leadership. In this, we emphasize complex problem solving, team interaction, team context and technology and team training design. These four factors represent significant barriers if neglected. On the other side, they may be considerable force multipliers when better understood and managed. We suggest how seven research and training questions could be linked to the four conceptual factors and guide an evidence-based approach to develop local crisis leadership.
Psychological and sociological determinants of entrepreneurial intentions and behaviors
Authors: Antončič Boštjan , Auer Antončič Jasna
Published: February 2023

Research concerned with the personality of entrepreneurs entails an important part of the research into the management of small and medium-sized enterprises and entrepreneurship. This research has added new knowledge about the role of entrepreneurs’ personality characteristics, their family entrepreneurial background, and the local supportive entrepreneurial background in entrepreneurial start-up intentions and behaviors. Hypotheses and a model were developed and verified using structural equation modeling and regression analysis considering data from a sample of entrepreneurs and students. This research revealed that several personality and sociological factors can be important for entrepreneurship when it comes to starting a business. The most important were the Big Five personality factors openness, extraversion, and non-agreeableness and, to a smaller extent, emotional stability (non-neuroticism), and conscientiousness. The second-most important group of factors were the specific motivational characteristics entrepreneurial self-efficacy, internal locus of control, and risk-taking propensity. Sociological factors were much less important than psychological elements for establishing business.
A study on surprisal and semantic relatedness for eye-tracking data prediction
Authors: Salicchi Lavinia , Chersoni Emmanuele , Lenci Alessandro
Published: February 2023

Previous research in computational linguistics dedicated a lot of effort to using language modeling and/or distributional semantic models to predict metrics extracted from eye-tracking data. However, it is not clear whether the two components have a distinct contribution, with recent studies claiming that surprisal scores estimated with large-scale, deep learning-based language models subsume the semantic relatedness component. In our study, we propose a regression experiment for estimating different eye-tracking metrics on two English corpora, contrasting the quality of the predictions with and without the surprisal and the relatedness components. Different types of relatedness scores derived from both static and contextual models have also been tested. Our results suggest that both components play a role in the prediction, with semantic relatedness surprisingly contributing also to the prediction of function words. Moreover, they show that when the metric is computed with the contextual embeddings of the BERT model, it is able to explain a higher amount of variance.
Metacognitive sensitivity and symptoms of mental disorder: A systematic review and meta-analysis
Authors: Hohendorf Marianne , Bauer Markus
Published: February 2023

Introduction

Metacognition is a term used to refer to cognition about cognitive processes. In this systematic review and meta-analysis, we reviewed studies that investigated the relationship between experimentally measured objective metacognitive sensitivity and diverse symptoms of mental disorder. In these studies, metacognitive sensitivity is operationalized as the correspondence between the accuracy of task performance and reported confidence therein.

Methods

A literature search was conducted across four databases and studies were selected for review based on predefined eligibility criteria. Twenty studies were included in the review and separate meta-analyses were conducted for psychotic and non-psychotic categories of psychiatric symptoms.

Results

A significant reduction (medium effect size) in metacognitive sensitivity was found in individuals with psychosis-related symptoms of mental disorder compared to healthy control groups, but no significant difference was found for individuals with non-psychotic symptoms. It should be noted though, that fewer studies were available for the latter group. Sub-group analysis found no evidence that the effect of metacognitive impairment depended on whether perceptual or non-perceptual experimental tasks were employed.

Discussion

These findings are discussed in relation to other conceptualizations of metacognition and the role reduced metacognitive sensitivity may play in forms of mental disorder.
Brain network changes in adult victims of violence
Authors: Shymanskaya Aliaksandra , Kohn Nils , Habel Ute , Wagels Lisa
Published: February 2023

Introduction

Stressful experiences such as violence can affect mental health severely. The effects are associated with changes in structural and functional brain networks. The current study aimed to investigate brain network changes in four large-scale brain networks, the default mode network, the salience network, the fronto-parietal network, and the dorsal attention network in self-identified victims of violence and controls who did not identify themselves as victims.

Materials and methods

The control group (n = 32) was matched to the victim group (n = 32) by age, gender, and primary psychiatric disorder. Sparse inverse covariance maps were derived from functional resting-state measurements and from T1 weighted structural data for both groups.

Results

Our data underlined that mostly the salience network was affected in the sample of self-identified victims. In self-identified victims with a current psychiatric diagnosis, the dorsal attention network was mostly affected underlining the potential role of psychopathological alterations on attention-related processes.

Conclusion

The results showed that individuals who identify themselves as victim demonstrated significant differences in all considered networks, both within- and between-network.
Development and validation of a nomogram to predict cancer-specific survival with unresected cholangiocarcinoma undergoing external radiotherapy
Authors: Song Jiazhao , Di Yupeng , Kang Xiaoli , Ren Gang , Wang Yingjie
Published: February 2023

Objective

To analyze the prognostic factors of patients with cholangiocarcinoma (CCA) who were unresected and received radiotherapy to establish a nomogram model for the prediction of patient cancer-specific survival (CSS).

Methods

Suitable patient cases were selected from the Surveillance, Epidemiology, and End Results (SEER) database, survival rates were calculated using the Kaplan-Meier method, prognostic factors were analyzed by Lasso, Cox regression, and nomogram was developed based on independent prognostic factors to predict 6 and 12 months CSS. The consistency index (C-index), calibration curve, and decision curve analysis (DCA) were tested for the predictive efficacy of the model, respectively.

Results

The primary site, tumor size, T-stage, M-stage, and chemotherapy (P < 0.05) were identified as independent risk factors after Cox and Lasso regression analysis. Patients in training cohort had a 6 months CSS rates was 68.6 ± 2.6%, a 12-month CSS rates was 49.0 ± 2.8%. The median CSS time of 12.00 months (95% CI: 10.17–13.83 months). The C-index was 0.664 ± 0.039 for the training cohort and 0.645 ± 0.042 for the validation cohort. The nomogram predicted CSS and demonstrated satisfactory and consistent predictive performance in 6 (73.4 vs. 64.9%) and 12 months (72.2 vs. 64.9%), respectively. The external validation calibration plot is shown AUC for 6- and 12-month compared with AJCC stage was (71.2 vs. 63.0%) and (65.9 vs. 59.8%). Meanwhile, the calibration plot of the nomogram for the probability of CSS at 6 and 12 months indicates that the actual and nomogram predict that the CSS remains largely consistent. DCA showed that using a nomogram to predict CSS results in better clinical decisions compared to the AJCC staging system.

Conclusion

A nomogram model based on clinical prognostic characteristics can be used to provide CSS prediction reference for patients with CCA who have not undergone surgery but have received radiotherapy.
The value of motherhood and psychological distress among infertile women: The mediating role of coping strategies
Authors: Foti Florentina Larisa , Karner-Huţuleac Adina , Maftei Alexandra
Published: February 2023

Introduction

The present study investigated psychological distress and coping strategies among infertile women during the COVID-19 pandemic using a multi-dimensional model of infertility-related stress. We explored the associations between individual (i.e., age) and situational characteristics related to infertility (i.e., duration of infertility, cause of infertility, number of lost pregnancies, and assisted reproductive techniques [ART] status), and perceived-infertility-related factors (i.e., the perceived importance of motherhood).

Methods

Our total sample consisted of 193 women aged 20 to 46 (M= 33.23, SD = 4.58), out of which 102 were undergoing ART procedures (M = 33.60, SD = 4.23), and 91 were not (M= 32.81, SD = 4.94). Participants filled in questionnaires measuring psychological distress, coping strategies, and the importance of motherhood.

Results

Correlation analyses suggested that the importance of motherhood was positively associated with psychological distress and negative coping strategies. Mediation analysis results indicated that both in the overall sample and in the sample of women undergoing ART procedures, the negative self-perception fully mediated the link between the importance of motherhood on psychological distress. In the non-ART sample, we found a significant mediation effect of denial on the link between the importance of motherhood and psychological distress.

Discussion

We discuss the theoretical and practical implications of the present findings, focusing on the mental health-related consequences of the social stigma of infertility heightened by the pressure of parenthood.
New experimental approaches enabling the continuous monitoring of gas species in hydrothermal fluids
Authors: Giroud Sébastien , Tomonaga Yama , Brennwald Matthias S. , Takahata Naoto , Shibata Tomo , Sano Yuji , Kipfer Rolf
Published: February 2023

Hot thermal fluids flow through the Earth's crust and carry valuable information about the deep subsurface. The monitoring of natural tracers transported in geothermal fluids, such as gases or ions, are relevant to better understand the geological processes in the Earth's subsurface and their relation to deep fluid dynamics. Recently developed technologies (e.g., portable gas-equilibrium membrane-inlet mass spectrometry) allow for the continuous monitoring of gas species at a much higher temporal resolution than the sampling procedures commonly used, based on a few individual samples. However, the monitoring of gas species from hot thermal fluids still poses experimental challenges tied to unwanted water vapor condensation in the headspace of the separation module, which irremediably leads to clogging (e.g., of the connecting capillaries) and failure of the detection device. In this contribution, we present two new experimental methods that provide suitable technical conditions to measure gases, even in high temperature geothermal fluids, using a portable gas analyzer. Two sites with different thermal water temperatures (first one ranging from 50 °C to 65 °C and second one close to boiling temperature) were selected. The first method was deployed on the thermal waters of Lavey-les-Bains (Vaud, Switzerland), for which we report results from October 2021. The second method was used in Beppu (Oita Prefecture, Japan), for which we report results from April 2018. Our results show that at both sites, our methods allow for continuous measurements of gas species (N2, Ar, O2, Kr, He, CH4, CO2 and H2) in thermal waters. Furthermore, they show that the variability of gas emanation from the two sites can only be adequately described by measurements with high temporal resolution, which both methods allow.
Eye Movements During Reading
Authors: Wegener Signy , Yu Lili , Reichle Erik D. , Beyersmann Elisabeth , Parrila Rauno , Castles Anne
Published: February 2023

Written words are everywhere. Learning to read is one of the main tasks of our early school years, and the ability to read opens up a world of possibilities—we can absorb ourselves in stories, remind ourselves of important information, and learn new things. But few of us think about what we are actually doing as we read. Moving the eyes is essential for reading. For instance, to read this sentence, you probably began by looking at the first word before moving your eyes to each word in turn. All the while, you are working hard to recognize and understand each word. In this article, you will learn about why eye movements are a necessary part of reading, how they are measured, what they tell scientists about what is happening in the mind during reading, and how they change as children grow into adults.
Bioactive volatile compounds from Penicillium digitatum-infected apples: Oviposition attractants for yellow peach moth Conogethes punctiferalis (Lepidoptera: Crambidae)
Authors: Guo Hong Gang , Miao Shuang Zhen , Ai Peng Peng , Zhang Min Zhao , Yan Zhe , Du Yan Li
Published: February 2023

Introduction

Plant-associated microbes critically shape the dynamics of plant-and insect-associated communities. In previous studies, we reported that the yellow peach moth Conogethes punctiferalis (YPM) preferred to Penicillium digitatum-infected apples (PDA) for oviposition. However, the underlying mechanisms remains unclear.

Methods

In the present study, the behavioral and physiological experiments were conducted to determine how P. digitatum affects the oviposition selection of mated YPM females via altering host plant volatile organic compounds (VOCs).

Results

Mated YPM females were attracted to and laid more eggs on PDA than on non-infected apples (NIA), mechanically damaged apples (MDA), and P. digitatum in potato dextrose agar medium (PPD) in the oviposition selection experiments. Four-arm olfactometer assays further confirmed that odors in PDA were responsible for the attractiveness of mated YPM females. Further analyses showed that 38 VOCs were collected and identified from all treatments by GC-MS, with five specific VOCs (methyl 2-methylbutyrate, styrene, methyl caproate, butyl caprylate, and n-tetradecane) emitting from PDA. A principal component analysis (PCA) based on the absolute contents of 38 VOCs revealed a clear separation of PDA from NIA, MDA, and PPD. Moreover, when P. digitatum-induced specific VOCs were added to apples in individual or synthetic blends, there was a significantly higher percentage of mated YPM females to apples with individual or synthetic blends consisting of methyl 2-methylbutyrate, butyl caprylate, or n-tetradecane in Y-tube olfactometer experiments, suggesting that these three specific VOCs acted as predominant olfactory signals for mated YPM females to PDA.

Discussion

Taken together, the microbe P. digitatum was an important driver of the interactions between YPMs and host plants by altering plant volatiles. These findings may form the basis for developing attractant baits for field trapping YPMs in the future.
First Insights on the Administration of Insect Oil (Black Soldier Fly Larvae) in the Diet of Juvenile Onychostoma macrolepis
Authors: Gou Nina , Wang Kaifeng , Jin Tiezhi , Yang Bin
Published: February 2023

The use of insect products in aquatic feed is the focus of the aquaculture industry. Black soldier fly larvae oil (BSFLO) has been examined as a potential lipid source for diets for some fish species, but its utilization on Onychostoma macrolepis has not been explored. The influences of substituting fish oil (FO) with BSFLO in the diet on growth, biological indicators, approximate composition, serum biochemistry, antioxidant capacity and expression of lipid metabolism genes in juvenile O. macrolepis were investigated in an eight-week feeding experiment. Four experimental diets were prepared by replacing 0 (control), 25% (BSFLO-25), 50% (BSFLO-50) and 100% (BSFLO-100) FO with BSFLO, and then randomly assigned to twelve aquariums with ten fish (1.75 ± 0.05 g) in each aquarium. The findings showed that the growth indicators, body composition and serum biochemistry in the BSFLO-25 and BSFLO-50 groups had no statistical differences from those of the control group. The activities of superoxide dismutase (SOD) (91.22–94.96 U/mgprot) and the contents of malondialdehyde (MDA) (1.12–1.16 nmol/mgprot) in the liver appeared to be significantly the highest and the lowest in the BSFLO-25, BSFLO-50 and BSFLO-100 groups (p < 0.05). The adipocyte size and intraperitoneal fat index value of fish fed on the BSFLO-100 diet were significantly decreased compared to the control group (p < 0.05). The expression levels of lipid catabolism genes pparα, (peroxisome proliferators-activated receptor alpha) and cpt1a (carnitine palmitoyltransferase 1 isoform a) in the BSFLO-100 group were significantly higher than those of the control group (p < 0.05). The above results indicated that it was feasible to replace 25–50% dietary FO with BSFLO in juvenile O. macrolepis. Dietary BSFLO supplementation could enhance the antioxidant capacity of the liver and suppress intraperitoneal fat accumulation in O. macrolepis. The use of other insect oils in the diets of this species will be evaluated in future research.
Effects of Partial Replacement of Soybean Meal with Defatted Hermetia illucens Meal in the Diet of Laying Hens on Performance, Dietary Egg Quality, and Serum Biochemical and Redox Indices
Authors: Zawisza Patrycja , Szymczyk Beata , Arczewska-Włosek Anna , Szczepanik Kinga
Published: February 2023

This study was carried out on 96 caged Bovans Brown laying hens at an initial age of 25 weeks, which were randomly assigned to four experimental groups of 12 replicates (cages) of two hens per cage. The control group hens received a diet containing 20% soybean meal (SBM), without Hermetia illucens larvae meal (HIM) content. The hens in the experimental groups received a diet containing defatted HIM at levels of 5, 10, and 15% (HIM 5%, HIM 10%, and HIM 15%, respectively), at the expense of a proportionally decreasing content of SBM. During the 12-week experiment, the laying performance, biochemical and redox blood indices, and liver condition were examined. The cholesterol level, fatty acid profile, and malondialdehyde content in egg yolks were also evaluated to determine the dietary quality of the eggs. The inclusion of HIM at any level in the diet did not affect the laying performance parameters (p > 0.05). Increased serum Ca and uric acid contents were observed. There was no effect on the redox indices in plasma. The number of hepatocytes was decreased in the HIM-fed groups. The level of cholesterol in yolks was reduced, and the fatty acid profile showed significant changes. Despite the high lauric acid content in the H. illucens meal, it was present in trace amounts in yolks. In the HIM-fed groups, the levels of saturated fatty acids increased significantly, whereas those of unsaturated fatty acids decreased in the yolks in the same groups.
Dairy Cows Grazing Plantain-Based Pastures Have Increased Urine Patches and Reduced Urine N Concentration That Potentially Decreases N Leaching from a Pastoral System
Authors: Nguyen Thi Truong , Navarrete Soledad , Horne David , Donaghy Daniel , Bryant Racheal H. , Kemp Peter
Published: February 2023

The objective of this study was to determine the effect of grazing plantain-based pastures on urine volume, urination frequency, and urinary nitrogen (UN) concentration of dairy cows under a typical pastoral dairy practice offering approximately 25% supplemented feeds. The experiment was a completely randomised design with three pasture treatments (perennial ryegrass–white clover (RGWC); RGWC + low plantain rate (PLL); and RGWC + high plantain rate (PLH)), five replicate plots, and repeated in two sequential grazing periods. Forty-five lactating Friesian × Jersey cows were randomly assigned into three groups of 15 animals each to graze over six days in adaptation paddocks and three days in experimental plots. Urine flow sensors were used to measure urine volume and urinary frequency, while spot urine sampling was conducted to determine nitrogen (N) concentration in cow urine. The results showed that including 25% plantain in the diet (PLH) increased daily urine volume by 44% and the daily number of urinations by 28%, compared to grazing the RGWC pasture. In addition, N concentration in cow urine was decreased by 18 and 29% when the diet contained 18% and 25% plantain, respectively. In conclusion, under a typical dairy farm practice, incorporating plantain into the RGWC pasture with the proportion of 25% plantain in the diet can increase the number of urine patches and reduce the concentration of N in the urine, thereby providing the opportunity to decrease N leaching from pastoral systems.
Energy-Performance Evaluation with Revit Analysis of Mathematical-Model-Based Optimal Insulation Thickness
Authors: Balo Figen , Ulutaş Alptekin
Published: February 2023

This study investigates the optimum insulation thickness value using MATLAB Optimization Toolbox based on a mathematical model for sandwich walls that are formed with different insulation-building materials by different fuel types for a particular city located in the second climatic region of Turkey. In the second stage of study, using the BIM-based Revit simulation program, a building was designed with the same building-insulation materials under the same climate conditions. The six different wall performances were compared for the designed building. The study proposes a comprehensive approach by combining technical and economic factors in the sustainable design of buildings. The computational results indicate that using different energy alternatives has a significant impact on the air quality in residential areas. The lowest value is reached when natural gas is used. The energy cost savings change from 7.56 to 14.12 TRY/m2 for external walls. While payback periods vary between 2.15 and 3.76 years for external walls, the lowest period for all wall types is obtained for electricity, which has a high cost. The optimum insulation thickness for 10 years of lifetime varies between 0.02 and 0.16 m. This study reflects that the highest optimal insulating thickness is reached when electricity is utilized as the energy source for all wall types. According to the Revit analysis, the lowest energy consumption of 21,677 kWh during one year using natural gas was obtained for a building material of porous light brick and an insulation material of glass wool.
An Enhancement Strategy for Macau’s Largos from a Syntactic Perspective
Authors: Lou Senyu , Zhang Yaou , Li Chao , Chen Jingjing
Published: February 2023

This paper takes the “Largos” of the Macau Peninsula as the object of study. By applying historical combing and field research, the main functions of Largos are categorized as follows: serving the community, facilitating traffic and promoting religious culture. The paper applied a spatial syntactic theory to establish a model, and three syntactic parameters were used, synergy, integration and choice, to interpret the results of the analysis. Based on the results of the syntactic parameters of Largos, results are divided into four categories. From this, the spaces that require improvements are selected, and corresponding improvement strategies are proposed. Taking Macau’s Largos as an example, this paper aimed to apply a space syntax to analyze the Largos in Macau so as to play a reference role in the effective renewal of urban micro-spaces and small public spaces.
2,2-Diphenethyl Isothiocyanate Enhances Topoisomerase Inhibitor-Induced Cell Death and Suppresses Multi-Drug Resistance 1 in Breast Cancer Cells
Authors: Aggarwal Monika
Published: February 2023

We previously reported that phenethyl isothiocyanate (PEITC), a dietary-related compound, can rescue mutant p53. A structure–activity relationships study showed that the synthetic analog 2,2-diphenylethyl isothiocyanate (DPEITC) is a more potent inducer of apoptosis than natural or synthetic ITCs. Here, we showed that DPEITC inhibited the growth of triple-negative breast cancer cells (MDA-MB-231, MDA-MB-468, and Hs578T) expressing “hotspot” p53 mutants, structural (p53R280K, p53R273H) or contact (p53V157F), at IC50 values significantly lower than PEITC. DPEITC inhibited the growth of HER2+ (p53R175H SK-BR-3, p53R175H AU565) and Luminal A (p53L194F T47D) breast cancer (BC) cells harboring a p53 structural mutant. DPEITC induced apoptosis, irrespective of BC subtypes, by rescuing p53 mutants. Accordingly, the rescued p53 mutants induced apoptosis by activating canonical WT p53 targets and delaying the cell cycle. DPEITC acted synergistically with doxorubicin and camptothecin to inhibit proliferation and induce apoptosis. Under these conditions, DPEITC delayed BC cells in the G1 phase, activated p53 canonical targets, and enhanced pS1981-ATM. DPEITC reduced the expression of MDR1 and ETS1. These findings are the first report of synergism between a synthetic ITC and a chemotherapy drug via mutant p53 rescue. Furthermore, our data demonstrate that ITCs suppress the expression of cellular proteins that play a role in chemoresistance.