bluuebunny/crossref_metadata_2025 huggingface dataset:
Building–Soil Thermal Interaction: A Case Study
Authors: Nawalany Grzegorz , Sokołowski Paweł
Published: July 2019

This paper presents an analysis of thermal interaction between a building and surrounding soil. The examined building was located in southern Poland. Measurements of selected indoor and outdoor air temperature parameters were made in order to determine the boundary conditions. The soil temperature measurements were conducted at 42 points. The analysis of results is divided into four periods: summer, autumn, winter, and spring. The analysis show that weather conditions significantly affect the temperature in soil, but the range of residential building impact decreases with distance, and it varies depending on the season. The residential building impact on the soil temperature is in the range of 1.2–3.3 m. This paper also includes a study of the heat flow direction in soil and a quantitative estimate of heat exchange between a building and the soil. The greatest energy losses 2082 kWh (21.24 kWh/m2) from the building to the soil were recorded in winter. In spring, the energy losses were reduced by about 38% as compared with the energy losses in winter, and the energy losses in spring were comparable to autumn.
Dust Removal from Solar PV Modules by Automated Cleaning Systems
Authors: Alghamdi Abdulsalam S. , Bahaj AbuBakr S. , Blunden Luke S. , Wu Yue
Published: July 2019

Dust accumulation on solar photovoltaic (PV) modules reduces light transmission from the outer surfaces to the solar cells reducing photon absorption and thus contributing to performance reduction of PV systems. In regions such as the Middle East where dust is prevalent and rainfall is scarce, remedial measures are needed to reduce such impacts. Currently, various techniques are being employed to address such sand soiling ranging from mechanical (brushing) to active and passive electrical interventions. This research focuses on mechanical approaches encompassing module vibration, air and water jets, and combinations of these. A reconfigurable pilot-scale testbed of 8 kWp PV plant was installed on a carport shading system within the campus of King Abdulaziz University (KAU), Jeddah, Saudi Arabia. The functional PV carport was configured to allow water recovery and re-use within the testbed. Here, we discuss the overall cleaning design philosophy and approach, systems design, and how multiple cleaning configurations can be realised within the overall PV carport. Results indicate that in this location, sand soiling has a significant effect on performance of PV modules on a timescale of days. In addition, water jets optimised for high volume and low pressure were effective at reducing sand soiling with array power output increasing by over 27%, whilst air jets and module vibration were less effective in reducing soiling to an acceptable level. Overall, the testbed has provided a new approach to testing a combination of cleaning solutions in the field coupled with used water recovery. The proposed approach is important, as currently, there are a large number of solar PV projects being built in Saudi Arabia with more being planned for the future.
Analyzing Spatial Distribution Patterns of European Beech (Fagus sylvatica L.) Regeneration in Dependence of Canopy Openings
Authors: Stiers Melissa , Willim Katharina , Seidel Dominik , Ammer Christian , Kabal Myroslav , Stillhard Jonas , Annighöfer Peter
Published: July 2019

The use of natural regeneration techniques is one of the key elements of modern (close-to-nature) forestry. In natural forests, changes in canopy cover, such as the emergence and successive re-closure of canopy gaps are particularly important, as they influence the light availability on the forest floor. Creating canopy gaps of different size is a promising silvicultural tool allowing the regulation of the light availability in managed forests in order to control regeneration composition and development. In this study, we used terrestrial laser scanning data to investigate the relationship between canopy-gap dimensions and emerging natural regeneration along a gradient of management in forests dominated by European beech (Fagus sylvatica L.). We analyzed the spatial distribution and height of regeneration patches in dependence of gap characteristics. Mean regeneration height decreases progressively from the gap polygon over a transition zone towards the area under the canopy, while the tallest regeneration plants were placed in positions midway between center and gap edge, and not directly in the gap center as we initially assumed. The centers of regeneration patches were not displaced when compared to the associated canopy gap centers, as has been reported in other studies conducted on the northern hemisphere for various tree species. The observed patterns did not depend on management strategies, indicating that regeneration responded equally to naturally created gaps and gaps that were caused by logging. We conclude that establishment and development of shade-tolerant European beech regeneration in forest stands is driven by gap openings, but not necessarily direct radiation. If at all, pronounced direct radiation mainly occurs at the northern edge of large gaps. Neither regeneration patch center, nor regeneration tree height pointed in that direction. Our study suggests that in the investigated beech-dominated forests the effect of increased light availability at the northern edge of a gap is overruled by other factors increasing towards the gap edge, such as increased belowground competition of the overstory trees.
Polysaccharide Hydrogels
Authors: Stokke Bjørn Torger
Published: July 2019

Polysaccharides are a unique source of organic materials in terms of abundance, structural diversity and functionalities [...]
Oxidative Stress Levels Induced by Mercury Exposure in Amazon Juvenile Populations in Brazil
Authors: Carvalho Leandro V.B. , Hacon Sandra S. , Vega Claudia M. , Vieira Jucilene A. , Larentis Ariane L. , Mattos Rita C. O. C. , Valente Daniel , Costa-Amaral Isabele C. , Mourão Dennys S. , Silva Gabriela P. , Oliveira Beatriz F. A.
Published: July 2019

Oxidative stress can be induced by mercury (Hg) exposure, including through fish consumption (diet), leading to health risks. The objective of this study was to evaluate the association between oxidative stress biomarkers and dietary Hg exposure levels in riverine children and adolescents at Madeira River (RO/Brazil). Population from three riverine local communities presenting different fish consumption frequencies was sampled. Hg was determined in blood (ICP-MS) and glutathione (GSH); glutathione S-transferases (GST) and malondialdehyde (MDA) were determined in serum (spectrophotometry). Statistical analyses were performed using parametric and non-parametric tests. Multiple linear regression models and generalized additives models were also used to estimate the relationships between oxidative stress biomarkers and blood Hg. The juvenile riverine population from Cuniã RESEX presented the highest levels of oxidative stress and Hg levels in blood (GST = 27.2 (4.93) U/L, MDA = 1.69 (0.27) µmol/L, Hg = 20.6 (18.0) µg/L). This population also presented the highest frequency of fish consumption. The positive relation between Hg and GST and MDA, adjusted for individual characteristics, suggests an oxidative effect. This study shows the importance of oxidative stress biomarkers in the evaluation of dietary Hg exposure since initial and reversible metabolic changes were observed, enriching health risk assessments.
A New Intervention Procedure for Improving Classroom Behavior of Neglected Children: Say Do Say Correspondence Training
Authors: Pino María J , Herruzo Javier , Herruzo Carlos
Published: July 2019

Although neglect is the most common form of child maltreatment, a review of the literature since 1980 reveals a lack of controlled child neglect intervention programs. The aim of this study is to assess a new intervention program to improve the classroom behavior of children exposed to neglect only, by reducing disruptive conduct and promoting adaptive conduct. Two matched groups were selected with children of the same ages, sex, and social class (cultural and economic level) and with mothers of similar ages. The experimental group comprised of five children suffering from neglect and no other type of maltreatment. The control group had five children not abused or neglected. All the children were in the same class at school. The percentage of time per session that each child spent engaged in disruptive behavior was measured (baseline) and was found significantly higher among neglected children. Say-Do-Say Correspondence Training was applied with the neglected children and a rapid, significant reduction in their disruptive behavior was observed (and statistically confirmed), bringing such behavior down to the level of the control (i.e., non-neglected) children. These results were maintained when the intervention was halted. We concluded that the adaptive and classroom behavior of neglected children can be improved with this non-intrusive intervention.
From Plant to Waste: The Long and Diverse Impact Chain Caused by Tobacco Smoking
Authors: Araújo Maria Christina B. , Costa Monica F.
Published: July 2019

Smoking is a social phenomenon of global scope. The impacts start from the cultivation of the plant to the disposal of cigarette butts in the most diverse places. These aspects go beyond economic and public health issues, also affecting natural environments and their biota in a serious and indistinct way. Of the six trillion cigarettes consumed globally each year, four and a half trillion are disposed somewhere in the environment. Cigarette butts are predominantly plastic, non-biodegradable waste, prevalent in coastal environments in various parts of the world, and with high potential for generating impacts on a wide range of socioeconomic and environmental aspects. Among the 5000 compounds found in a cigarette, those with higher toxic potential are mainly concentrated in the filter and in tobacco remnants, which are items found in discarded cigarette butts. After surveying published studies on this topic, the present study addressed the interaction between the impacts related to tobacco smoking, highlighting the problem as an important and emerging issue that demands joint efforts, and actions especially focused on the reduction of environmental impacts, an aspect that has not yet been assessed.
Investigating the Effects of Time and Temperature on the Growth of Escherichia coli O157:H7 and Listeria monocytogenes in Raw Cow’s Milk Based on Simulated Consumer Food Handling Practices
Authors: Leclair Roselyn M. , McLean Sarah K. , Dunn Louise A. , Meyer Denny , Palombo Enzo A.
Published: July 2019

Consumption of raw cow’s milk (RCM) is increasing in popularity in developed countries despite the associated foodborne disease risks. While previous research has focused on consumer motivations for drinking RCM, there is limited research on how consumer handling practices may impact the microbiological safety of RCM. In this study, consumer handling practices associated with transport, storage, and freezing and thawing were simulated to investigate the impact of time and temperature variables on the concentrations of either Escherichia coli O157:H7 or Listeria monocytogenes in RCM. We found that the type of storage during simulated transport had a large (η2 = 0.70) and significant (p < 0.001) effect on both pathogens. The refrigeration temperature also had a large (η2 = 0.43) and significant (p < 0.001) effect on both pathogens during refrigerated storage. The interaction between pathogen species and initial pathogen inoculum level had a large (η2 = 0.20) and significant (p = 0.012) effect on the concentration of the pathogens during ambient temperature storage. We found that freezing and thawing practices did not have a significant effect on the pathogens (p > 0.05). However, we were able to recover L. monocytogenes, but not E. coli O157:H7, from RCM after freezing for 365 days. The results from this study highlight that consumer transport and storage practices can have significant effects on the growth of E. coli O157:H7 and L. monocytogenes in RCM. Consumer food handling practices should be considered when developing public health strategies aimed at reducing the risks of RCM consumption.
Living in the Restoration Public Housing after the Great East Japan Earthquake Correlates with Lower Subjective Well-Being of Older Adults
Authors: Moriyama Nobuaki , Iwasa Hajime , Tsubokura Masaharu , Kuroda Yujiro , Yasumura Seiji
Published: July 2019

We aimed to (1) describe the subjective well-being (SWB) of older residents in Fukushima Prefecture seven years and seven months after the Great East Japan Earthquake (GEJE) and examine the effect of relocation to the restoration public housing (RPH) on SWB, social capital, and health indicators; and (2) investigate the association between social capital and SWB. Questionnaires were administered to collect data of both RPH and non-RPH residents (≥65 years). Respondents’ SWB was collected via the Japanese version of the World Health Organization Five Well-Being Index. Additionally, residents’ social capital (trust, reciprocity, and participation), physical activity level, social network, functional health, history of chronic disease, and demographic data were collected. We analyzed 101 responses (valid response rate: approximately 34%) from RPH and 158 (53%) from non-RPH residents. SWB was lower in RPH compared to non-RPH residents but not statistically significant. Older RPH residents may demonstrate lower social capital and health indicators after the GEJE. Mistrust was found to be positively associated with low SWB in RPH residents. Future studies should examine the effectiveness of support for enhancing the trust of older RPH residents regarding, for example, the involvement of scientists—including medical professionals—in risk communications in promoting SWB.
Influence of Smoking Status on Risk of Incident Heart Failure: A Systematic Review and Meta-Analysis of Prospective Cohort Studies
Authors: Lee Hyeonju , Son Youn-Jung
Published: July 2019

Smoking is a well-known risk factor for atherosclerotic cardiovascular disease. However, there are insufficient data regarding the predictive influence of smoking status on the risk of incident heart failure (HF). This study involved a systematic review and meta-analysis of prospective cohort studies to identify the association of smoking status with incident risk of HF. Peer-reviewed articles published in PubMed, Embase, Web of Science, Cochrane, and CINAHL up to May 2019 were identified. Seven studies, based on 42,759 participants and 4826 HF cases, were included. Pooled hazard ratios (HRs) and their 95% confidence intervals (CI) were estimated using the fixed effects model. Subgroup analyses were conducted to define possible sources of heterogeneity. Current smokers aged 18 years and over had a greater risk of HF incidence compared with non-smokers (never or former smokers) (HR = 1.609, 95% CI, 1.470–1.761). Additionally, former smokers had a greater risk of HF incidence compared with never smokers (HR = 1.209, 95% CI, 1.084–1.348). The present study highlighted that never smokers have more obvious cardiovascular benefits than current or former smokers. Therefore, health professionals should support cessation at the earliest among current smokers and encourage young people and non-smokers not to start smoking.
CRISPR/Cas Applications in Myotonic Dystrophy: Expanding Opportunities
Authors: Raaijmakers Renée H.L. , Ripken Lise , Ausems C. Rosanne M. , Wansink Derick G.
Published: July 2019

CRISPR/Cas technology holds promise for the development of therapies to treat inherited diseases. Myotonic dystrophy type 1 (DM1) is a severe neuromuscular disorder with a variable multisystemic character for which no cure is yet available. Here, we review CRISPR/Cas-mediated approaches that target the unstable (CTG•CAG)n repeat in the DMPK/DM1-AS gene pair, the autosomal dominant mutation that causes DM1. Expansion of the repeat results in a complex constellation of toxicity at the DNA level, an altered transcriptome and a disturbed proteome. To restore cellular homeostasis and ameliorate DM1 disease symptoms, CRISPR/Cas approaches were directed at the causative mutation in the DNA and the RNA. Specifically, the triplet repeat has been excised from the genome by several laboratories via dual CRISPR/Cas9 cleavage, while one group prevented transcription of the (CTG)n repeat through homology-directed insertion of a polyadenylation signal in DMPK. Independently, catalytically deficient Cas9 (dCas9) was recruited to the (CTG)n repeat to block progression of RNA polymerase II and a dCas9-RNase fusion was shown to degrade expanded (CUG)n RNA. We compare these promising developments in DM1 with those in other microsatellite instability diseases. Finally, we look at hurdles that must be taken to make CRISPR/Cas-mediated editing a therapeutic reality in patients.
A Novel CreA-Mediated Regulation Mechanism of Cellulase Expression in the Thermophilic Fungus Humicola insolens
Authors: Xu Xinxin , Fan Chao , Song Liya , Li Jinyang , Chen Yuan , Zhang Yuhong , Liu Bo , Zhang Wei
Published: July 2019

The thermophilic fungus Humicola insolens produces cellulolytic enzymes that are of great scientific and commercial interest; however, few reports have focused on its cellulase expression regulation mechanism. In this study, we constructed a creA gene (carbon catabolite repressor gene) disruption mutant strain of H. insolens that exhibited a reduced radial growth rate and stouter hyphae compared to the wild-type (WT) strain. The creA disruption mutant also expressed elevated pNPCase (cellobiohydrolase activities), pNPGase (β-glucosidase activities), and xylanase levels in non-inducing fermentation with glucose. Unlike other fungi, the H. insolens creA disruption mutant displayed lower FPase (filter paper activity), CMCase (carboxymethyl cellulose activity), pNPCase, and pNPGase activity than observed in the WT strain when fermentation was induced using Avicel, whereas its xylanase activity was higher than that of the parental strain. These results indicate that CreA acts as a crucial regulator of hyphal growth and is part of a unique cellulase expression regulation mechanism in H. insolens. These findings provide a new perspective to improve the understanding of carbon catabolite repression regulation mechanisms in cellulase expression, and enrich the knowledge of metabolism diversity and molecular regulation of carbon metabolism in thermophilic fungi.
The Protective Effect of Insulin on Rat Cortical Neurons in Oxidative Stress and Its Dependence on the Modulation of Akt, GSK-3beta, ERK1/2, and AMPK Activities
Authors: Zakharova Irina O. , Sokolova Tatiana V. , Bayunova Liubov V. , Zorina Inna I. , Rychkova Maria P. , Shpakov Alexander O. , Avrova Natalia F.
Published: July 2019

Insulin is a promising drug for the treatment of diseases associated with brain damage. However, the mechanism of its neuroprotective action is far from being understood. Our aim was to study the insulin-induced protection of cortical neurons in oxidative stress and its mechanism. Immunoblotting, flow cytometry, colorimetric, and fluorometric techniques were used. The insulin neuroprotection was shown to depend on insulin concentration in the nanomolar range. Insulin decreased the reactive oxygen species formation in neurons. The insulin-induced modulation of various protein kinase activities was studied at eight time-points after neuronal exposure to prooxidant (hydrogen peroxide). In prooxidant-exposed neurons, insulin increased the phosphorylation of GSK-3beta at Ser9 (thus inactivating it), which resulted from Akt activation. Insulin activated ERK1/2 in neurons 5–30 min after cell exposure to prooxidant. Hydrogen peroxide markedly activated AMPK, while it was for the first time shown that insulin inhibited it in neurons at periods of the most pronounced activation by prooxidant. Insulin normalized Bax/Bcl-2 ratio and mitochondrial membrane potential in neurons in oxidative stress. The inhibitors of the PI3K/Akt and MEK1/2/ERK1/2 signaling pathways and the AMPK activator reduced the neuroprotective effect of insulin. Thus, the protective action of insulin on cortical neurons in oxidative stress appear to be realized to a large extent through activation of Akt and ERK1/2, GSK-3beta inactivation, and inhibition of AMPK activity increased by neuronal exposure to prooxidant.
Gene-Wide Analysis of Aquaporin Gene Family in Malus domestica and Heterologous Expression of the Gene MpPIP2;1 Confers Drought and Salinity Tolerance in Arabidposis thaliana
Authors: Liu Haili , Yang Leilei , Xin Miaomiao , Ma Fengwang , Liu Jingying
Published: July 2019

The aquaporins (AQPs) are a family of integral membrane proteins involved in the transcellular membrane transport of water and other small molecules. A scan of the apple (Malus domestica) genome revealed the presence of 42 genes encoding putative AQPs. Based on a phylogenetic analysis of the deduced peptide sequences of the AQPs generated by Arabidopsis thaliana, poplar (Populus trichocarpa), and rubber (Hevea brasiliensis), the apple AQPs were each assigned membership of the five established AQP subfamilies, namely the PIPs (eleven members), the TIPs (thirteen members), the NIPs (eleven members), the SIPs (five members), and the XIPs (two members). The apple AQPs included asparagine-proline-alanine (NPA) motifs, an aromatic/arginine (ar/R) selectivity filter, and the Froger’s positions. The heterologous expression of MpPIP2;1 in A. thaliana was shown to enhance the level of tolerance exhibited against both drought and salinity.
Mucin Thin Layers: A Model for Mucus-Covered Tissues
Authors: Rondelli Valeria , Di Cola Emanuela , Koutsioubas Alexandros , Alongi Jenny , Ferruti Paolo , Ranucci Elisabetta , Brocca Paola
Published: July 2019

The fate of macromolecules of biological or pharmacological interest that enter the mucus barrier is a current field of investigation. Studies of the interaction between the main constituent of mucus, mucins, and molecules involved in topical transmucoidal drug or gene delivery is a prerequisite for nanomedicine design. We studied the interaction of mucin with the bio-inspired arginine-derived amphoteric polymer d,l-ARGO7 by applying complementary techniques. Small angle X-ray scattering in bulk unveiled the formation of hundreds of nanometer-sized clusters, phase separated from the mucin mesh. Quartz microbalance with dissipation and neutron reflectometry measurements on thin mucin layers deposited on silica supports highlighted the occurrence of polymer interaction with mucin on the molecular scale. Rinsing procedures on both experimental set ups showed that interaction induces alteration of the deposited hydrogel. We succeeded in building up a new significant model for epithelial tissues covered by mucus, obtaining the deposition of a mucin layer 20 Å thick on the top of a glycolipid enriched phospholipid single membrane, suitable to be investigated by neutron reflectometry. The model is applicable to unveil the cross structural details of mucus-covered epithelia in interaction with macromolecules within the Å discreteness.
An Innovative Stochastic Multi-Agent-Based Energy Management Approach for Microgrids Considering Uncertainties
Authors: Ghorbani Sajad , Unland Rainer , Shokouhandeh Hassan , Kowalczyk Ryszard
Published: July 2019

In microgrids a major share of the energy production comes from renewable energy sources such as photovoltaic panels or wind turbines. The intermittent nature of these types of producers along with the fluctuation in energy demand can destabilize the grid if not dealt with properly. This paper presents a multi-agent-based energy management approach for a non-isolated microgrid with solar and wind units and in the presence of demand response, considering uncertainty in generation and load. More specifically, a modified version of the lightning search algorithm, along with the weighted objective function of the current microgrid cost, based on different scenarios for the energy management of the microgrid, is proposed. The probability density functions of the solar and wind power outputs, as well as the demand of the households, have been used to determine the amount of uncertainty and to plan various scenarios. We also used a particle swarm optimization algorithm for the microgrid energy management and compared the optimization results obtained from the two algorithms. The simulation results show that uncertainty in the microgrid normally has a significant effect on the outcomes, and failure to consider it would lead to inaccurate management methods. Moreover, the results confirm the excellent performance of the proposed approach.
Interfacial Stress Analysis of Adhesively Bonded Lap Joint
Authors: Her Shiuh-Chuan , Chan Cheng-Feng
Published: July 2019

The use of adhesively bonded joints in place of traditional joining techniques such as bolted or rivet joints is becoming greatly popular in recent years. Interfacial stress in the adhesive is critical to the strength of adhesively bonded joints. It is necessary to predict the interfacial stresses accurately to ensure the safety of joints. In this work, an analytical model is explicitly presented to evaluate the stresses in a double lap joint. The equilibrium equations in the adhesive overlap region are derived on the basis of elasticity theory. The governing equations are presented in terms of shear and peel stresses in the adhesive. Analytical solutions are derived for the shear and peel stresses, which are considered to be the main reason for the failure of the double lap joint. To verify the analytical solutions, the finite element method is conducted using the commercial package ANSYS. Results from the analytical solution agree well with finite element results and numerical investigations available in the literature. The effect of the adhesive thickness, shear modulus, adherend Young’s modulus and bonding length on the shear and peel stresses in the adhesive of the double lap joint are studied. Numerical results demonstrate that both the maximum shear and peel stress occur at both ends of the bonding region. The maximum values of the shear and peel stresses increase as the adhesive thickness decreases and as the adhesive shear modulus increases provided that the adhesive thickness is sufficiently small. The simplicity and capability to obtain analytical expressions of the shear and peel stresses for double lap adhesive bonded joints makes the proposed analytical model applicable for the stress analysis and preliminary structural design.
Preparation and Microwave Absorption Properties of C@Fe3O4 Magnetic Composite Microspheres
Authors: Shi Youqiang , Yin Yanan , Zhang Yi , Hu Yue , Liu Weifeng
Published: July 2019

In this work, C@Fe3O4 magnetic microspheres were designed and prepared by a novel strategy, and the microwave absorption properties of the materials were investigated. Four kinds of monodisperse P(MAA/St) microspheres with different carboxyl content were synthesized via facile dispersion polymerization. The Fe3O4 nanoparticles were grown on the surface of P(MAA/St) to obtain P(MAA/St)@Fe3O4 microspheres. Using P(MAA/St)@Fe3O4 as the precursors, after vacuum carbonization, C@Fe3O4 were obtained. It was observed that the carboxyl content on the microspheres’ surface increased with the increasing of MAA, which made the magnetic content and maximum specific saturation magnetization of P(MAA/St)@Fe3O4 and C@Fe3O4 increase. The obtained four kinds of C@Fe3O4 microspheres had a particle size range of 4–6 μm. The microwave absorption properties indicated that the magnetic content made a difference to the microwave absorption properties of C@Fe3O4 magnetic microspheres. The microwave absorption properties of materials were determined by controlling dielectric loss, magnetic loss and impedance matching. C@Fe3O4 microspheres exhibited excellent microwave absorption properties. The maximum reflection loss could reach −45.6 dB at 12.8 GHz with 3 mm in thickness. The effective bandwidth was 5.9 GHz with RL < −10 dB. Therefore, C@Fe3O4 microspheres were lightweight and efficient microwave absorption materials.
Biocompatibility and Clinical Application of Porous TiNi Alloys Made by Self-Propagating High-Temperature Synthesis (SHS)
Authors: Yasenchuk Yuri , Marchenko Ekaterina , Gunther Victor , Radkevich Andrey , Kokorev Oleg , Gunther Sergey , Baigonakova Gulsharat , Hodorenko Valentina , Chekalkin Timofey , Kang Ji-hoon , Weiss Sabine , Obrosov Aleksei
Published: July 2019

Porous TiNi alloys fabricated by self-propagating high-temperature synthesis (SHS) are biomaterials designed for medical application in substituting tissue lesions and they were clinically deployed more than 30 years ago. The SHS process, as a very fast and economically justified route of powder metallurgy, has distinctive features which impart special attributes to the resultant implant, facilitating its integration in terms of bio-mechanical/chemical compatibility. On the phenomenological level, the fact of high biocompatibility of porous SHS TiNi (PTN) material in vivo has been recognized and is not in dispute presently, but the rationale is somewhat disputable. The features of the SHS TiNi process led to a multifarious intermetallic Ti4Ni2(O,N,C)-based constituents in the amorphous-nanocrystalline superficial layer which entirely conceals the matrix and enhances the corrosion resistance of the unwrought alloy. In the current article, we briefly explore issues of the high biocompatibility level on which additional studies could be carried out, as well as recent progress and key fields of clinical application, yet allowing innovative solutions.
In Vitro Comparison of Biocompatibility of Calcium Silicate-Based Root Canal Sealers
Authors: Lee Ju Kyung , Kim Sunil , Lee Sukjoon , Kim Hyeon-Cheol , Kim Euiseong
Published: July 2019

The aim of this study was to assess the effect of three calcium silicate-based sealers (EndoSeal MTA, Nano-ceramic Sealer, and Wellroot ST) and two epoxy resin-based sealers (AH-Plus, AD Seal) on various aspects, such as cell viability, inflammatory response, and osteogenic potential, of human periodontal ligament stem cells (hPDLSCs). AH-Plus showed the lowest cell viability on hPDLSCs in all time periods in fresh media. In set media, hPDLSCs showed no significant differences in cell viability among all the tested materials. Wellroot ST showed the highest level of cell adhesion and the morphology of attached cells. AH-plus presented a significantly higher expression of IL-6 and IL-8 than the other sealers. AD Seal and three calcium silicate sealers showed high expression of the mesenchymal stem cell markers. ALP mRNA expression showed a significant increase in time-dependent manner on all of three calcium silicate-based sealers, which do not seem to interfere with the differentiation of hPDLSCs into osteoblasts. Based on the results from this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy resin-based sealers. Meanwhile, further and long-term clinical follow-up studies are required.
Study of Helium Swelling in Nitride Ceramics at Different Irradiation Temperatures
Authors: Zdorovets Maxim. V. , Dukenbayev Kanat , Kozlovskiy Artem. L.
Published: July 2019

This paper presents the results of a systematic study of helium swelling and the subsequent process of degradation of the near-surface layer of aluminum-based nitride ceramics. The samples were irradiated with 40 keV He2+ ions at temperatures of 300 and 1000 K with a fluence of 1 × 1017–5 × 1017 ions/cm2. The choice of radiation doses and temperature conditions was due to the possibility of simulating reactor tests of structural materials. It has been established that an increase in the irradiation fluence leads to the formation of large agglomerates of clusters of helium bubbles, as well as an increase in the degree of roughness and waviness of the surface with the formation of crater-like inclusions. In the case of irradiation at high temperatures, there was a slight decrease in the average size of helium inclusions compared with irradiation at room temperature. However, the density of inclusions and surface roughness were much higher. It is established that irradiation at room temperatures leads to a sharp decrease in ceramics density, as well as deformation of the crystal structure due to an increase in the density of dislocations and macrostresses in the structure. The decrease in ceramics density due to the formation of helium inclusions led to an increase in porosity and a defective fraction in the structure of the surface layer of ceramics.
Constitutive Modeling of the Tensile Behavior of Recycled Polypropylene-Based Composites
Authors: Wang Kui , Peng Yong , Matadi Boumbimba Rodrigue , Bahlouli Nadia , Pessey Daniel , Ahzi Said , Addiego Frédéric , Rémond Yves
Published: July 2019

The effect of reprocessing on the quasi-static uniaxial tensile behavior of two commercial polypropylene (PP)-based composites is experimentally investigated and modeled. In particular, the studied materials consist of an unfilled high-impact PP and a talc-filled high-impact PP. These PP composites are subjected to repeated processing cycles, including a grinding step and an extrusion step to simulate recycling at the laboratory level, the selected reprocessing numbers for this study being 0, 3, 6, 9, and 12. Because the repeated reprocessing leads to thermo-mechanical degradation by chain scission mechanisms, the tensile behavior of the two materials exhibits a continuous decrease of elastic modulus and failure strain with the increasing amount of reprocessing. A physically consistent three-dimensional constitutive model is used to predict the tensile response of non-recycled materials with strain rate dependence. For the recycled materials, the reprocessing effect is accounted by incorporating the reprocessing sensitive coefficient into the constitutive model for Young’s modulus, failure strain, softening, and hardening equations. Our predictions of true stress—true strain curves for non-recycled and recycled 108MF97 and 7510—are in good agreement with experimental data and can be useful for industries and companies which are looking for a model able to predict the recycling effect on mechanical behavior of polymer-based materials.
Effect of Ultraviolet–Ozone Treatment on the Properties and Antibacterial Activity of Zinc Oxide Sol-Gel Film
Authors: Kim Ji-Hyeon , Ma Junfei , Lee Seunghun , Jo Sungjin , Kim Chang Su
Published: July 2019

To combat infectious diseases, zinc oxide (ZnO) has been identified as an effective antibacterial agent; however, its performance can be adversely affected by harsh application environments. The ozone impact on ZnO antibacterial film needs to be evaluated prior to its application in an ozone disinfection system. In this study, ZnO films synthesized via sol-gel/spin-coating were subjected to ultraviolet–ozone (UVO) treatment for different periods. Surface investigations using scanning electron microscopy, ultraviolet–visible spectroscopy, and X-ray photoelectron spectroscopy revealed that the treatment-induced film changes. With longer UVO treatment, the surface porosity of the film gradually increased from 5% to 30%, causing the transmittance reduction and absorbance increase in visible-light range. Phase transformation of Zn(OH)2 to ZnO occurred during the first 10 min of UVO treatment, followed by oxygen uptake as a consequence of the reaction with reactive oxygen species generated during UVO treatment. However, despite these surface changes, the satisfactory antibacterial activity of the synthesized ZnO film against Staphylococcus aureus and Escherichia coli was sustained even after 120 min of UVO treatment. This indicates that the UVO-induced surface changes do not have a significant effect on the antibacterial performance and that the ZnO sol-gel film possesses good functional durability in ozone environments.
New Trisulfated Steroids from the Vietnamese Marine Sponge Halichondria vansoesti and Their PSA Expression and Glucose Uptake Inhibitory Activities
Authors: Tabakmakher Kseniya M. , Makarieva Tatyana N. , Denisenko Vladimir A. , Popov Roman S. , Dmitrenok Pavel S. , Dyshlovoy Sergey A. , Grebnev Boris B. , Bokemeyer Carsten , von Amsberg Gunhild , Cuong Nguyen X.
Published: July 2019

Seven new unusual polysulfated steroids—topsentiasterol sulfate G (1), topsentiasterol sulfate I (2), topsentiasterol sulfate H (3), bromotopsentiasterol sulfate D (4), dichlorotopsentiasterol sulfate D (8), bromochlorotopsentiasterol sulfate D (9), and 4β-hydroxyhalistanol sulfate C (10), as well as three previously described—topsentiasterol sulfate D (7), chlorotopsentiasterol sulfate D (5) and iodotopsentiasterol sulfate D (6) have been isolated from the marine sponge Halichondria vansoesti. Structures of these compounds were determined by detailed analysis of 1D- and 2D-NMR and HRESIMS data, as well as chemical transformations. The effects of the compounds on human prostate cancer cells PC-3 and 22Rv1 were investigated.
Discovery of Two New Sorbicillinoids by Overexpression of the Global Regulator LaeA in a Marine-Derived Fungus Penicillium dipodomyis YJ-11
Authors: Yu Jing , Han Huan , Zhang Xianyan , Ma Chuanteng , Sun Chunxiao , Che Qian , Gu Qianqun , Zhu Tianjiao , Zhang Guojian , Li Dehai
Published: July 2019

Overexpression of the global regulator LaeA in a marine-derived fungal strain of Penicillium dipodomyis YJ-11 induced obvious morphological changes and metabolic variations. Further chemical investigation of the mutant strain afforded a series of sorbicillinoids including two new ones named 10,11-dihydrobislongiquinolide (1) and 10,11,16,17-tetrahydrobislongiquinolide (2), as well as four known analogues, bislongiquinolide (3), 16,17-dihydrobislongiquinolide (4), sohirnone A (5), and 2′,3′-dihydrosorbicillin (6). The results support that the global regulator LaeA is a useful tool in activating silent gene clusters in Penicillium strains to obtain previously undiscovered compounds.
Characterization and Immunomodulatory Effects of High Molecular Weight Fucoidan Fraction from the Sporophyll of Undaria pinnatifida in Cyclophosphamide-Induced Immunosuppressed Mice
Authors: Yoo Hee Joon , You Dong-Ju , Lee Kwang-Won
Published: July 2019

Immunomodulation involves two mechanisms, immunostimulation and immunosuppression. It is a complex mechanism that regulates the pathophysiology and pathogenesis of various diseases affecting the immune system. Immunomodulators can be used as immunostimulators to reduce the side effects of drugs that induce immunosuppression. In this study, we characterized the chemical composition of high molecular weight fucoidan (HMWF) and low molecular weight fucoidan and compared their functions as natural killer (NK) cell-derived immunostimulators in vitro. We also tested the effectiveness of HMWF, which has a relatively high function in vitro, as an immunostimulator in immunosuppressed animal models. In these models, HWMF significantly restored NK cell cytotoxicity and granzyme B release to the control group level. In addition, the expression of interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α also increased in the spleen. This study suggests that HMWF acts as an effective immunostimulant under immunosuppressive conditions.
HDL-C Role in Acquired Aortic Valve Stenosis Patients and Its Relationship with Oxidative Stress
Authors: Hofmanis Juris , Hofmane Dace , Svirskis Simons , Mackevics Vitolds , Tretjakovs Peteris , Lejnieks Aivars , Signorelli Salvatore Santo
Published: July 2019

Background and objectives: Mechanical stress is currently considered as the main factor promoting calcific aortic valve stenosis (AS) onset. It causes endothelial damage and dysfunction. The chronic inflammatory process causes oxidative stress. Oxidative stress-induced high-density lipoprotein cholesterol (HDL-C) dysfunction is an important component of the development of AS. The aim of the study was to evaluate the role of HDL-C in AS patients in three severity grades and in relation to the biomarkers of oxidative stress, thioredoxin reductase 1 (TrxR1) and myeloperoxidase (MPO). Materials and Methods: 18 patients with mild, 19 with moderate. and 15 with severe AS were included in the study, and 50 individuals were enrolled in the control group. Stenosis severity was determined by echocardiography. The TrxR1 and MPO were analyzed by ELISA, and HDL-C by commercially available tests. Data were analyzed using GraphPad Prism 8. Results: HDL-C in AS patients vs. control substantially decreases and this decline was observed in all three AS severity groups: mild (p = 0.018), moderate (p = 0.0002), and severe (p = 0.004). In both the control and the stenosis group, the HDL-C was higher in women than in men. In comparison to control, the HDL-C level was lower in the AS group, and more pronounced in women (p = 0.0001) than in men (p = 0.049). A higher TrxR1 level was observed in patients with mild (p = 0.0001) and severe AS (p = 0.047). However, a clear correlation between TrxR1 and HDL-C was not obtained. Analysis of MPO showed differences in all severity grades vs. control (p = 0.024 mild stenosis; p = 0.002 moderate stenosis; p = 0.0015 severe stenosis). A negative correlation (p = 0.047; rp = −0.28) was found between MPO and HDL-C, which confirms the adverse effects of MPO resulting in HDL-C dysfunction. Conclusions: In this study, we justified HDL-C level association with AS development process. The results unequivocally substantiated the association between HDL-C and AS in all severity grades in women, but only in moderate AS for men, which we explained by the small number of men in the groups. The obtained correlation between the HDL-C and MPO levels, as well as the concurrent decrease in the HDL-C level and increase in the TrxR1 level, indicate in general an HDL-C association with oxidative stress in AS patients.
Biotransformation of the Mycotoxin Enniatin B1 by CYP P450 3A4 and Potential for Drug-Drug Interactions
Authors: Ivanova Lada , Denisov Ilia G. , Grinkova Yelena V. , Sligar Stephen G. , Fæste Christiane K.
Published: July 2019

Enniatins (ENNs) are fungal secondary metabolites that frequently occur in grain in temperate climates. Their toxic potency is connected to their ionophoric character and lipophilicity. The biotransformation of ENNs predominantly takes place via cytochrome P450 3A (CYP 3A)-dependent oxidation reactions. Possible interaction with ENNs is relevant since CYP3A4 is the main metabolic enzyme for numerous drugs and contaminants. In the present study, we have determined the kinetic characteristics and inhibitory potential of ENNB1 in human liver microsomes (HLM) and CYP3A4-containing nanodiscs (ND). We showed in both in vitro systems that ENNB1 is mainly metabolised by CYP3A4, producing at least eleven metabolites. Moreover, ENNB1 significantly decreased the hydroxylation rates of the typical CYP3A4-substrate midazolam (MDZ). Deoxynivalenol (DON), which is the most prevalent mycotoxin in grain and usually co-occurrs with the ENNs, was not metabolised by CYP3A4 or binding to its active site. Nevertheless, DON affected the efficiency of this biotransformation pathway both in HLM and ND. The metabolite formation rates of ENNB1 and the frequently used drugs progesterone (PGS) and atorvastatin (ARVS) lactone were noticeably reduced, which indicated a certain affinity of DON to the enzyme with subsequent conformational changes. Our results emphasise the importance of drug–drug interaction studies, also with regard to natural toxins.
Development of a Novel Three Degrees-of-Freedom Rotary Vibration-Assisted Micropolishing System Based on Piezoelectric Actuation
Authors: Gu Yan , Chen Xiuyuan , Lu Faxiang , Lin Jieqiong , Yi Allen , Feng Jie , Sun Yang
Published: July 2019

The limited degrees of freedom (DOF) and movement form of the compliant vibration-assisted processing device are inherent constraints of the polishing technique. In this paper, a concept of a 3-DOF rotary vibration-assisted micropolishing system (3D RVMS) is proposed and demonstrated. The 3-DOF means the proposed vibration-assisted polishing device (VPD) is driven by three piezo-electric (PZT) actuators. Compared with the current vibration-assisted polishing technology which generates a trajectory with orthogonal actuators or parallel actuators, a novel 3-DOF piezoelectrically actuated VPD was designed to enable the workpiece to move along the rotational direction. Meanwhile, the proposed VPD can deliver large processing stoke in mrad scale and can be operated at a flexible non-resonant mode. A matrix-based compliance modeling method was adopted for calculating the compliance and amplification ratio of the VPD. Additionally, the dynamic and static properties of the developed VPD were verified using finite element analysis. Then, the VPD was manufactured and experimentally tested to investigate its practical performance. Finally, various polished surfaces which used silicon carbide (SiC) ceramic as workpiece material were uniformly generated by the high-performance 3D RVMS. Compared with a nonvibration polishing system, surface roughness was clearly improved by introducing rotary vibration-assisted processing. Both the analysis and experiments verified the effectiveness of the present 3D RVMS for micro-machining surfaces.
Impact of Spray-Dried Plasma on Intestinal Health and Broiler Performance
Authors: Campbell Joy M. , Crenshaw Joe D. , González-Esquerra Ricardo , Polo Javier
Published: July 2019

Spray-dried plasma (SDP) is a functional ingredient commonly utilized in swine production and calf milk replacers to improve performance, feed efficiency, and health. The improvements noted with SDP in animal production are more pronounced under commercial production conditions compared to cleaner research settings. Multiple modes of action of SDP have been proposed, including either directly influencing the immune inflammatory response locally or systemically, and/or through the indirect modification of beneficial microbial populations. Spray-dried plasma included at various dietary levels and duration of feeding in broilers has been evaluated in different production and challenging conditions with beneficial effects on broiler performance, as noted in other animals. The purpose of this review is to discuss research related to the modes of action of SDP on immunomodulation and improved intestinal health and specifically discuss research conducted utilizing SDP in feed for poultry. Collectively, the data available indicates that SDP improves early intestinal health and supports an efficient immune system response both locally at the intestine and systemically, thereby benefiting growth, feed efficiency, and survival of broilers in conventional commercial production and under challenging conditions such as disease or environmental stressors.