bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Vinylotrimethoxysilane-Modified Linseed Oil on Selected Properties of Impregnated Wood
Authors: Perdoch Waldemar , Depczyńska Ewelina , Tomkowiak Karolina , Furgał Monika , Kurczak Mariola , Mazela Bartłomiej
Published: August 2022

This study aimed to examine the effect of organosilicon compounds-modified linseed oil parameters on wood utility properties. Linseed oil silylation with an organosilicon compound containing a vinyl group (vinyltrimethoxysilane) has made it possible to synthesize products characterized by high stability in storage. The impregnation of wood with these resulting products contributed to increased resistance of the protected wood to water exposure when compared to unprotected wood or wood protected by polymerized oil that was not subject to the silylation reaction. The protected wood was characterized by a lower water absorption rate in liquid as well as vapor forms contained in the humid air. This property immediately translated into an increase in the resistance of the wood exposed to Basidiomycota. The loss of mass of Pinus sylvestris L. (pine wood) exposed to the Coniophora puteana was approximately 3%. This parameter did not improve when the wood was subjected to the washing process (mass loss approx. 3.6%). Thus, the resulting products are those demonstrating the highest potential for use in the preparation of wood protection means.
The Soil Aggregates and Associated Organic Carbon across the Greater Khingan Mountains: Spatial Patterns and Impacting Factors
Authors: Wang Bing , Gong Zhiqi , Meng Meng , Zhang Qiuliang
Published: August 2022

(1) Background: The preservation of soil organic carbon (SOC) by soil aggregates (SA) is a key mechanism for the stability of the soil carbon (C) pool. (2) Methods: Soil samples were collected at a 0–20 cm depth from 75 sites across the forest regions of the Greater Khingan Mountains, China, and were fractionated as SA of 0.25–2 mm, 0.053–0.25 mm, and <0.053 mm by a wet-sieving method. The spatial patterns of SA and associated organic C (OC) were investigated, as well as their associations with environmental factors. (3) Results: The predominant SA was the SA fraction (SAF) of 0.25–2 mm. The spatial pattern of SA, with moderate spatial autocorrelation, was found to be associated with aggregate size. SOC was mainly accumulated in the 0.25–2 mm SAF, accounting for 50.39% of the total content of aggregate SOC; the total SOC content in all SAF showed strong spatial autocorrelations without significant differences. No significant correlations were found between temperature and SA variables. Precipitation presented significantly positive and negative correlations with the SAF of <0.053 mm and 0.053–0.25 mm, respectively. SOC was favorably associated with macroaggregate, geometric mean diameter (GMD), and mean weight diameter (MWD); however, the correlation between SOC and aggregate SOC lessened as particle size decreased. The aggregate SOC contents were significantly linked with NH4+-N, AK, and TP, while 0.25–2 mm SA content was negatively related with pH. (4) Conclusions: Precipitation was helpful for the development of clay aggregates (<0.053 mm), i.e., increasing rainfall-induced aggregate disruption. SOC encourages small SA cementing to large SA, which improves SA stability. OC associated with the SAF of 0.25–2 mm and 0.053–0.25 mm was positively correlated with soil nutrients such as nitrogen, phosphorus, and potassium, suggesting that the formation of aggregates was conducive to the preservation of soil nutrients.
Increases in the Methane Uptake of Upland Forest Soil in China Could Significantly Contribute to Climate Change Mitigation
Authors: Yang Meng
Published: August 2022

Upland forest soil is an important CH4 sink that plays a key role in climate change mitigation. China features large areas of various types of forest, but spatiotemporal variation in CH4 flux has not yet been clarified. Here, we analyzed variation in CH4 flux and the effects of environmental variables on the CH4 flux of forest in China using in situ observational data. Upland forest soil absorbed CH4 at a rate of 0.24 ± 0.02 g m−2 yr−1. The CH4 uptake rate (0.46 ± 0.10 g m−2 yr−1) of warm temperate deciduous broad-leaved forest was the highest. Soil alkali-hydrolyzable nitrogen was the only factor significantly correlated with CH4 uptake variation among vegetation zones. A break point in CH4 uptake over the study period (from 1997 to 2020) was detected in 2015. CH4 uptake slightly decreased until 2015 and increased after 2015. The mean CH4 uptake of the period after 2015 (0.44 ± 0.07 g m−2 yr−1) was significantly higher than that before 2015 (0.20 ± 0.02 g m−2 yr−1). Atmospheric nitrogen deposition was negatively related to interannual CH4 uptake. Our findings suggest that the CH4 uptake of upland forest soil will continue to increase over the next few decades as China accelerates efforts to achieve its carbon neutrality goal, and this would result in continuous decreases in nitrogen deposition through various pathways.
Effects of the Autochthonous Probiotic Bacteria Lactobacillus plantarum B and Lactococcus lactis Subsp. lactis S1 on the Proteolysis of Croatian Cheese Ripened in a Lambskin Sack (Sir iz Mišine)
Authors: Vrdoljak Marija , Tudor Kalit Milna , Dolenčić Špehar Iva , Radeljević Biljana , Jelić Marko , Mandinić Sandra , Frece Jadranka , Kalit Samir
Published: August 2022

This study aims to determine the effects of the autochthonous probiotic bacteria Lactobacillus plantarum B (currently Lactiplantibacillus plantarum) and Lactococcus lactis subsp. lactis S1 on proteolysis during the ripening of Sir iz mišine—a Croatian cheese which ripens in a lambskin sack. Sir iz mišine was produced in four different variants: (1) from raw milk without starter cultures, and from pasteurized milk with added (2) Lactococcus lactis ssp. lactis S1, (3) Lactobacillus plantarum B, or (4) a starter culture consisting of a mixture of Lactococcus lactis ssp. lactis S1 and Lactobacillus plantarum B (1:1). The addition of Lactobacillus plantarum B alone or in combination with Lactococcus lactis subsp. lactis S1 noticeably increased the alpha and beta indices because of the synergistic activity between the enzymes responsible for primary proteolysis and added autochthonous bacteria. Cheese produced from raw milk had the lowest (12.16%) content of WSN%TN. The highest WSN%TN content was found in cheese produced with combined probiotic bacteria (30.40%) and Lactococcus lactis ssp. lactis S1 (29.74%). Cheese with added combined probiotic bacteria had a noticeably higher content of TCA-SN%TN, indicating a synergistic performance among autochthonous probiotic bacteria. In conclusion, autochthonous probiotic bacteria, in addition to having a functional value, can improve the ripening properties of cheese.
Effect of Fermentation on the Biochemical Parameters Antioxidant Capacity and Dispersed Composition of Plant Beverages Based on Barley and Hemp Seeds
Authors: Merenkova Svetlana , Fatkullin Rinat , Kalinina Irina
Published: August 2022

Enzymatic processes play a key role in the production of grain-containing food due to their effect on the nutritional properties, rheological characteristics, and contribution to improving the functional and antioxidant proprieties. Eight samples of beverages based on barley grain and hemp seeds were produced (control beverages and beverages fermented by bifidobacteria and propionic acid bacteria). It was found that lactic acid accumulated during fermentation alongside a gradual shift in the pH level in the acidic direction. A comparative analysis of the DPPH activity revealed the highest values for barley-based beverages, ranging from 71.0 to 100.7%, while for the hemp seed-based beverages, the DPPH activity was 64.1–97.9%. The maximum values of DPPH activity were observed during fermentation with a combination of bifidobacteria and propionic acid bacteria concentrates. The highest concentration of polyphenolic compounds and flavonoids was found in barley-based beverages fermented with Propionibacterium freudenreichii (1.26 mg GAE/g and 0.11 mg EQ/g) and a combination of Propionibacterium freudenreichii and Bifidobacterium longum (1.24 mg GAE/g and 0.14 mg EQ/g). Studies have shown an increase in the nutrient content for fermented beverages compared to the control samples. The barley-based beverages exhibited the largest average dynamic particle diameter, and all beverage samples showed a more uniform particle size distribution after microbial fermentation.
Data-Driven Analysis of Outdoor-to-Indoor Propagation for 5G Mid-Band Operational Networks
Authors: Ali Usman , Caso Giuseppe , De Nardis Luca , Kousias Konstantinos , Rajiullah Mohammad , Alay Özgü , Neri Marco , Brunstrom Anna , Di Benedetto Maria-Gabriella
Published: August 2022

The successful rollout of fifth-generation (5G) networks requires a full understanding of the behavior of the propagation channel, taking into account the signal formats and the frequencies standardized by the Third Generation Partnership Project (3GPP). In the past, channel characterization for 5G has been addressed mainly based on the measurements performed on dedicated links in experimental setups. This paper presents a state-of-the-art contribution to the characterization of the outdoor-to-indoor radio channel in the 3.5 GHz band, based on experimental data for commercial, deployed 5G networks, collected during a large scale measurement campaign carried out in the city of Rome, Italy. The analysis presented in this work focuses on downlink, outdoor-to-indoor propagation for two operators adopting two different beamforming strategies, single wide-beam and multiple synchronization signal blocks (SSB) based beamforming; it is indeed the first contribution studying the impact of beamforming strategy in real 5G networks. The time and power-related channel characteristics, i.e., mean excess delay and Root Mean Square (RMS) delay spread, path loss, and K-factor are studied for the two operators in multiple measurement locations. The analysis of time and power-related parameters is supported and extended by a correlation analysis between each pair of parameters. The results show that beamforming strategy has a marked impact on propagation. A single wide-beam transmission leads, in fact, to lower RMS delay spread and lower mean excess delay compared to a multiple SSB-based transmission strategy. In addition, the single wide-beam transmission system is characterized by a smaller path loss and a higher K-factor, suggesting that the adoption of a multiple SSB-based transmission strategy may have a negative impact on downlink performance.
A 3D CFD-Based Workflow for Analyses of a Wide Range of Flow and Heat Transfer Conditions in Air Gaps of Electric Machines
Authors: Žnidarčič Anton , Katrašnik Tomaž
Published: August 2022

Increasing power densities of electric machines in e-vehicles in addition to the resulting quest for enhanced cooling concepts are bringing forward the importance of defining adequate heat transfer correlations in air gaps. This is a highly challenging topic, as there exist no generally applicable flow and heat transfer phenomena descriptions for air gaps due to their highly variable geometrical properties and operating conditions. As an answer to this challenge, this paper presents a workflow that defines an adequate 3D CFD model for an arbitrary air-gap design that includes its system-dependent boundary conditions. The workflow is built on the recognition of underlying air-gap flow phenomena, which are used to steer the subsequent design of the 3D CFD model in a systematic step-by-step manner. Consequently, the complexity of the 3D CFD model gradually increases to the point where it provides an adequate flow and heat transfer description. Validation of the workflow is presented for a wide range of air-gap designs and flow conditions. It is demonstrated that the 3D CFD models obtained with the workflow match the experimentally obtained data from various flow cases that have been documented in the literature. Considerable optimization of computational costs, offering potentially an order-of-magnitude reduction in computational time, is achieved as a result of computational domain span optimization and transient simulations being applied only when required. The validation confirms that this workflow facilitates construction of valid 3D CFD models without the prior knowledge of flow and heat transfer phenomena in a specific air gap. This workflow thus provides a reliable and computationally efficient tool for valorization of convective heat transfer, and opens up prospects for time- and cost-efficient optimizations of electric machines’ cooling system designs.
Probability Distribution of Extreme Events in a Baroclinic Wave Laboratory Experiment
Authors: Harlander Uwe , Borcia Ion Dan , Vincze Miklos , Rodda Costanza
Published: August 2022

Atmospheric westerly jet streams are driven by temperature differences between low and high latitudes and the rotation of the Earth. Meandering jet streams and propagating Rossby waves are responsible for the variable weather in the mid-latitudes. Moreover, extreme weather events such as heat waves and cold spells are part of the jet stream dynamics. For many years, a simple analog in the form of a simplified laboratory experiment, the differentially heated rotating annulus, has provided insight into the dynamics of the meandering jet stream. In the present study, probability density distributions of extreme events from a long-term laboratory experiment are studied and compared to the atmospheric probability density distributions. Empirical distributions of extreme value monthly block data are derived for the experimental and atmospheric cases. Generalized extreme value distributions are adjusted to the empirical distributions, and the distribution parameters are compared. Good agreement was found, but the distributions of the experimental data showed a shift toward larger extreme values, and some explanations for this shift are suggested. The results indicate that the laboratory model might be a useful tool for investigating changes in extreme event distributions due to climate change. In the laboratory context, the change can be modeled by an increase in total temperature accompanied by a reduction in the radial heat contrast.
Response Surface Optimization of Solar Drying Conditions and the Effect on the Quality Attributes and Drying Characteristics of Qula Casein
Authors: Wang Jun , Wang Lina , Wang Linlin , Han Ling , Chen Lianhong , Tang Shanhu , Wen Pengcheng
Published: August 2022

The objective of this study was to investigate the potential application of a mixed-mode solar dryer to Qula dehydration in the Qinghai-Tibet Plateau of China. A three-factor five-level central composite rotatable design (CCD) of the response surface methodology (RSM) was employed to evaluate the influences of drying process variables on quality attributes in terms of lipid oxidation [peroxide (POV) and thiobarbituric acid reactive substance (TBARS)] and Maillard reaction (5-hydroxymethylfurfural, 5-HMF). The impact of drying temperature (30–50 °C), material thickness (5–15 mm), and wind velocity (0.4–1.4 m/s) on the color, POV, TBARS, and 5-HMF of Qula were studied. Optimum operating conditions were found to be a temperature of 43.0 °C, material thickness of 11.0 mm, and wind velocity of 1.0 m/s based on the minimum of POV, TBARS, and 5-HMF. In this condition, the values of POV, TBARS, and 5-HMF were 0.65 meq/kg, 0.516 mg/kg, and 4.586 mg water/L, respectively, which are significantly lower than for drying of Qula by open sun-drying (p < 0.05). Compared with open sun-drying, the drying time of Qula by solar drying was shortened by 61.5%. The results indicate that the mixed-mode solar dryer is a feasibility drying technology for Qula and could effectively improve the quality of products.
Solutions of Initial Value Problems with Non-Singular, Caputo Type and Riemann-Liouville Type, Integro-Differential Operators
Authors: Angstmann Christopher N. , Burney Stuart-James M. , Henry Bruce I. , Jacobs Byron A.
Published: August 2022

Motivated by the recent interest in generalized fractional order operators and their applications, we consider some classes of integro-differential initial value problems based on derivatives of the Riemann–Liouville and Caputo form, but with non-singular kernels. We show that, in general, the solutions to these initial value problems possess discontinuities at the origin. We also show how these initial value problems can be re-formulated to provide solutions that are continuous at the origin but this imposes further constraints on the system. Consideration of the intrinsic discontinuities, or constraints, in these initial value problems is important if they are to be employed in mathematical modelling applications.
An EHPB1L1 Nonsense Mutation Associated with Congenital Dyserythropoietic Anemia and Polymyopathy in Labrador Retriever Littermates
Authors: Shelton G. Diane , Minor Katie M. , Guo Ling T. , Thomas-Hollands Alison , Walsh Koranda A. , Friedenberg Steven G. , Cullen Jonah N. , Mickelson James R.
Published: August 2022

In this report, we describe a novel genetic basis for congenital dyserythropoietic anemia and polymyopathy in Labrador Retriever littermates characterized by incidental detection of marked microcytosis, inappropriate metarubricytosis, pelvic limb weakness and muscle atrophy. A similar syndrome has been described in English Springer Spaniel littermates with an early onset of anemia, megaesophagus, generalized muscle atrophy and cardiomyopathy. Muscle histopathology in both breeds showed distinctive pathological changes consistent with congenital polymyopathy. Using whole genome sequencing and mapping to the CanFam4 (Canis lupus familiaris reference assembly 4), a nonsense variant in the EHBP1L1 gene was identified in a homozygous form in the Labrador Retriever littermates. The mutation produces a premature stop codon that deletes approximately 90% of the protein. This variant was not present in the English Springer Spaniels. Currently, EHPB1L1 is described as critical to actin cytoskeletal organization and apical-directed transport in polarized epithelial cells, and through connections with Rab8 and a BIN1-dynamin complex generates membrane vesicles in the endocytic recycling compartment. Furthermore, EHBP1L1 knockout mice die early and develop severe anemia. The connection of EHBP1L1 to BIN1 and DMN2 functions is particularly interesting due to BIN1 and DMN2 mutations being causative in forms of centronuclear myopathy. This report, along with an independent study conducted by another group, are the first reports of an association of EHBP1L1 mutations with congenital dyserythropoietic anemia and polymyopathy.
Association of Hypoglycemia with Biomarkers of Oxidative Stress and Antioxidants: An Observational Study
Authors: Papachristoforou Eleftheria , Kountouri Aikaterini , Maratou Eirini , Kouretas Dimitris , Skaperda Zoi , Tsoumani Maria , Efentakis Panagiotis , Ikonomidis Ignatios , Lambadiari Vaia , Makrilakis Konstantinos
Published: August 2022

Hypoglycemia has been associated with complications from the vasculature. The contributing effects of oxidative stress (OS) on these actions have not been sufficiently studied, especially in daily, routine clinical practice. We examined the association of hypoglycemia encountered in daily clinical practice with biomarkers of OS and endogenous antioxidant activity in persons with diabetes [type 1 (T1D) or type 2 (T2D)], as well as individuals without diabetes, with a history of hypoglycemia. Several biomarkers of OS (MDA, ADMA, ox-LDL, 3-NT, protein carbonyls, 4-HNE, TBARS) and antioxidant capacity (TAC, superoxide scavenging capacity, hydroxyl radical scavenging capacity, reducing power, ABTS) were measured. Blood was drawn at the time of hypoglycemia detection and under euglycemic conditions on a different day. A total of 31 participants (mean age [±SD] 52.2 ± 21.1 years, 45.2% males) were included in the study. There were 14 (45.2%) persons with T2D, 12 (38.7%) with T1D, and 5 (16.1%) without diabetes. We found no differences in the examined biomarkers. Only TBARS, a biomarker of lipid peroxidation, showed lower values during hypoglycemia (p = 0.005). This finding needs confirmation in more extensive studies, given that MDA, another biomarker of lipid peroxidation, was not affected. Our study suggests that hypoglycemia encountered in daily clinical practice does not affect OS.
Effects of Fusarium proliferatum on Aboveground Physiological Indicators of Superior Apple Rootstock Line 12-2 (Malus spectabilis) with Improved Apple-Replant-Disease Resistance
Authors: Cui Xueli , Qin Xin , Liu Yangbo , Hu Yanli , Mao Zhiquan , Chen Xuesen , Mao Yunfei , Shen Xiang
Published: August 2022

(1) Background: Cultivating resistant rootstocks is an effective way to mitigate apple replant disease (ARD), and we developed superior apple rootstock line 12-2 (self-named), which shows improved ARD resistance. (2) Methods: We used ARD-associated pathogen Fusarium proliferatum MR5 (MR5) to test the fungal infection in the 12-2 line. Seedlings of the 12-2, T337, and M26 rootstock lines were planted in a substrate with potato dextrose broth and MR5 spore solution, and aboveground physiological indicators were measured. (3) Results: MR5 had the greatest effect on the leaf growth of T337 and M26. The incidence rates of infectious symptoms in the T337 and M26 lines were 68 and 100%, respectively. MR5 significantly affected the leaf chlorophyll content, ETR, and NPQ of T337 and M26, as well as Pn and Tr of M26. MR5 tended to reduce the leaf photosynthetic parameters of T337, but the decreases were not significant. The leaf reactive-oxygen-species levels of T337 and M26, the leaf antioxidant-enzyme activities of M26, and the superoxide-dismutase activity of T337 were significantly affected by MR5. MR5 also had a significant effect on the leaf malondialdehyde, proline, and soluble-sugar contents of T337 and M26. None of these aboveground physiological indicators were affected by MR5 in the 12-2 rootstock. (4) Conclusions: The 12-2 rootstock was more resistant to ARD-associated MR5 and could serve as an important test material for resistant-apple-rootstock breeding in China.
Subjective Social Status, Area Deprivation, and Gender Differences in Health among Chinese Older People
Authors: Chen Xi , Woo Jean , Yu Ruby , Chung Gary Ka-Ki , Yao Wei , Yeoh Eng-Kiong
Published: August 2022

This study examined the gender differences in the main and interactive effects of subjective social status and area deprivation on health among older adults in Hong Kong. Data for this study came from the baseline of MrOs and MsOs studies, including 4000 Chinese men and women ≥ 65 in Hong Kong. Subjective social status was assessed using the MacArthur Scale of subjective social status scale. Our results reaffirm that subjective social status is an independent indicator of health after adjusting for objective SES measures (e.g., education and income). Perceived rank on the community ladder was more closely related to health among older people than was the society ladder, particularly for women. Although area-level social deprivation was not significantly associated with the health of older people, it may moderate the effect of subjective social status on health. Women with a lower perceived status in the community were more likely to experience depressive symptoms but better grip strength when living in more deprived neighborhoods. The findings suggested that subjective social status provides important information for the physical and mental health of the older population. Policymakers may implement interventions to enhance the subjective social status of older adults. Given the greater contribution of relative status in the community to the health of women, these policies and interventions should target to improve women’s perceived status in the community.
Effect on Perceived Weight of Object Shapes
Authors: Ryu Taebeum , Park Jaehyun , Bitkina Olga Vl.
Published: August 2022

The perceived weight of an object is an important research topic in terms of sensation and perception, and it is known that it has size-weight, color-weight, and material-weight illusions due to the influence of size, color, and material, as well as the weight of the object. Although the physical size of an object is measured by volume, the size of an object that we subjectively feel depends on the shape of the object, even if it has the same volume. Therefore, the shape of the object may determine the perceived size of the object, thereby changing its perceived weight accordingly. These cognitive factors play an important role in the period of rehabilitation therapy after an exacerbation or attack of neurological diseases, such as stroke or Parkinson’s disease, regarding the motor functions of the patient. Moreover, the study of these sensation and perception factors is important for the period of the early development of children, for example, for tracking and correcting fine motor skills. Existing related studies analyzed the perceived weight according to three shapes (tetrahedron, cube, and sphere), but only some shapes showed a difference in the perceived weight. This study attempted to demonstrate the difference in perceived weight according to the shape that has yet to be clearly identified. To this end, this study investigated objects with the same physical size (volume) as in previous studies, but in the shapes of tetrahedron, cube, and sphere. In addition, the volumes of these objects were set to 64,000 cm3, 125,000 cm3, and 216,000 cm3, and their weights were set to be 100 g, 150 g, and 200 g, in proportion to the size of the small, medium, and large volumes, respectively. Thirty-eight college students (21 males, 17 females) participated and the perceived weight of a given object compared to a reference object was evaluated according to the modulus method used for sensory size measurement. The analysis of the experimental data found that both weight (volume) and shape had significant effects on the perceived weight. The results support that the shape of objects also led to the size-weight illusion phenomenon. At the same weight (volume), the perceived weight of an object according to shape decreased significantly in the order of sphere, cube, and tetrahedron. At the same volume level, subjective size according to shape is small in the order of tetrahedron, cube, and sphere. The results of weight perception according to shape in this study showed that the subjective size of an object according to shape had an effect on perceived weight.
The Survey Measure of Psychological Safety and Its Association with Mental Health and Job Performance: A Validation Study and Cross-Sectional Analysis
Authors: Sasaki Natsu , Inoue Akiomi , Asaoka Hiroki , Sekiya Yuki , Nishi Daisuke , Tsutsumi Akizumi , Imamura Kotaro
Published: August 2022

Objectives: This study validated the Japanese version of O’Donovan et al.’s (2020) composite measure of the psychological safety scale and examined the associations of psychological safety with mental health and job-related outcomes. Methods: Online surveys were administered twice to Japanese employees in teams of more than three members. Internal consistency and test–retest reliability were tested using Cronbach’s α and intra-class correlation coefficient (ICC), respectively. Structural validity was examined using confirmatory factor analysis (CFA) and exploratory factor analysis (EFA). Convergent validity was tested using Pearson’s correlation coefficients. Multiple linear regression analyses were conducted to examine the relationship between psychological safety and psychological distress, work engagement, job performance, and job satisfaction. Results: Two hundred healthcare workers and 200 non-healthcare workers were analyzed. Internal consistency, test–retest reliability, and convergent validity were acceptable. CFA demonstrated poor fit, and EFA yielded a two-factor structure, with team leader as one factor and peers and team forming the second factor. The total score showed significant and expected associations with all outcomes in the adjusted model for all workers. Conclusions: The Japanese version of the measure of the psychological safety scale presented good reliability and validity. Psychological safety is important for employees’ mental health and performance.
The Sociodemographic-Professional Profile and Emotional Intelligence in Infant and Primary Education Teachers
Authors: Arteaga-Cedeño Wendy L. , Carbonero-Martín Miguel Á. , Martín-Antón Luis J. , Molinero-González Paula
Published: August 2022

Emotional intelligence is the key to students’ psychological-social well-being and academic performance, and teachers must provide socioemotional education in the classroom. To achieve this, teachers must display high levels of socioemotional skills that ensure their own personal, social, and career well-being and, as a result, that of their students. This study seeks to gain an insight into the levels of socioemotional skills of emotional perception, emotional understanding, and emotional regulation and how these are linked to the sociodemographic and career profile of teachers in infant and primary education. For this, we surveyed 351 teachers—310 female and 41 male—aged between 20 and 69. We used the Trait Meta-Mood Scale-24 (TMMS-24) together with a questionnaire (ad hoc) to determine the sociodemographic and career profile of participating teachers. Statistical analysis of the data showed that gender impacts on emotional regulation and emotional perception, while age and number of the children were also seen to have significant difference on emotional regulation and emotional perception. In contrast, professional qualifications were only seen to affect emotional perception. The variable reflecting the level at which staff teach showed significant difference on emotional perception and emotional understanding, while administrative posts held by teachers also demonstrated significant difference on their emotional understanding. The results confirm that sociodemographic and work-related variables impact the level of socioemotional skills of infant and primary education teachers. These aspects should be taken into account in the structure and planning of training aimed at developing socioemotional skills in order to ensure their success.
Mutational Assessment in NKX2-5 and ACTC1 Genes in Patients with Congenital Cardiac Septal Defect (CCSD) from Ethnic Kashmiri Population
Authors: Nazeer Nadeem Ul , Bhat Mohammad Akbar , Rah Bilal , Bhat Gh Rasool , Wani Shadil Ibrahim , Yousuf Adfar , Dar Abdul Majeed , Afroze Dil
Published: August 2022

(1) Background globe. The etiology of CHDs is complex and involves both genetic and non-genetic factors. Although, significant progress has been made in deciphering the genetic components involved in CHDs, recent reports have revealed that mutations in Nk2 homeobox5 (NKX2-5) and actin alpha cardiac muscle1 (ACTC1) genes play a key role in CHDs such as atrial and ventricular septum defects. Therefore, the present study evaluates the role of key hotspot mutations in NKX2-5 and ACTC1 genes of congenital cardiac septal defect (CCSD) in ethnic Kashmiri population. (2) Methods: A total of 112 confirmed CHD patients were included in the current study, of which 30 patients were evaluated for mutational analysis for hotspot mutations of NKX2-5 and ACTC1 genes. The total genomic DNA was extracted from the samples (cardiac tissue/blood) and were subjected to amplification for NKX2-5 (exon 1 and 2), and ACTC1 (exon 2) genes by using PCR specific primers to analyze the hotspot mutations in respective exons. The amplified products obtained were sent to Macrogen Korea for sequencing by Sanger’s method. (3) Results: Our results confirmed that not a single mutation was found in either hotspot exon 1 and 2 of NKX2-5 and exon 2 of ACTC1 in the patients included in the current study. Interestingly, a novel synonymous nucleotide variation leading to G > C transversion (GCG > GCC) was found in exon 2 of NKX2-5 gene of CCSD patient. (4) Conclusions: The current findings demonstrated the role of NKX2-5 and ACTC1 in cardiac development. The study will provide an insight in understanding the genetic etiology and highlights the role of newly identified mutations in patients with CDS’s in ethnic Kashmiri population. In silico findings revealed amino acid changes, splice site variation and the creation of new site. Furthermore, the study warrants complete screening of genes involved in CCSDs.
Evaluation of the Carbon Sink Capacity of the Proposed Kunlun Mountain National Park
Authors: Zhao Li , Du Mingxi , Du Wei , Guo Jiahuan , Liao Ziyan , Kang Xiang , Liu Qiuyu
Published: August 2022

National parks, as an important type of nature protected areas, are the cornerstone that can effectively maintain biodiversity and mitigate global climate change. At present, China is making every effort to build a nature-protection system, with national parks as the main body, and this approach considers China′s urgent goals of obtaining carbon neutrality and mitigating climate change. It is of great significance to the national carbon-neutralization strategy to accurately predict the carbon sink capacity of national park ecosystems under the background of global change. To evaluate and predict the dynamics of the carbon sink capacity of national parks under climate change and different management measures, we combined remote-sensing observations, model simulations and scenario analyses to simulate the change in the carbon sink capacity of the proposed Kunlun Mountain National Park ecosystem over the past two decades (2000–2020) and the change in the carbon sink capacity under different zoning controls and various climate change scenarios from 2020 to 2060. Our results show that the carbon sink capacity of the proposed Kunlun Mountain National Park area is increasing. Simultaneously, the carbon sink capacity will be improved with the implementation of park management and control measures; which will be increased by 2.04% to 2.13% by 2060 in the research area under multiple climate change scenarios. The research results provide a scientific basis for the establishment and final boundary determination of the proposed Kunlun Mountain National Park.
The Spaces of Peer-Led Support Groups for Suicide Bereaved in Denmark and the Republic of Ireland: A Focus Group Study
Authors: Hybholt Lisbeth , Higgins Agnes , Buus Niels , Berring Lene Lauge , Connolly Terry , Erlangsen Annette , Morrissey Jean
Published: August 2022

Research has shown that people bereaved by suicide have an increased risk of mental health problems, suicidality and associated stigma, as well as higher rates of sick leave and increased rates of receiving disability pensions. Peer-led suicide bereavement support groups are perceived to enhance people’s recovery by enabling shared experiences with others who are bereaved in similar circumstances. The aim of the research was to explore the viewpoints of participants living in Denmark and the Republic of Ireland on these peer-led support groups. This study focused on how the participants experienced being part of the peer-led support and how the participation affected them. We conducted four focus groups, two in Denmark and two in the Republic of Ireland, and two individual interviews, involving a total of 27 people bereaved by suicide. Data were analyzed thematically. The participants’ experiences in the peer-led support groups were in contrast to what they had experienced in their daily lives. They felt alienated in daily living, as they believed that people could not comprehend their situation, which in turn led participants to search for people with similar experiences and join the peer-led support groups. While peer-led support groups may not be helpful for everyone, they created ‘supportive spaces’ that potentially affected the participants’ recovery processes, from which we generated three key themes: (i) ‘A transformative space’, describing how the peer-led support group created a place to embrace change, learning and knowledge about suicide and suicide bereavement and the making of new connections; (ii) ‘An alternative space for belonging and grieving’, describing how the participants felt allowed to and could give themselves permission to grieve; and (iii) ‘A conflicted space’ describing how it was troublesome to belong to and participate in the peer-led support groups. In conclusion, despite the two cultural settings and different organizational approaches, the experiences were comparable. Peer-led support groups can, despite being a conflicted space for some, provide supportive spaces aiding the participants’ recovery process.
The Wellbeing of Chinese Migrating Grandparents Supporting Adult Children: Negotiating in Home-Making Practices
Authors: Zhu Dan , Xu Haichao , Yao Yuan
Published: August 2022

Health geography provides a relational approach to understanding elders’ wellbeing experience in relation to place. That the migrating grandparents move between their home and their adult children’s home to support their children’s life in the migrating city provides a particular pattern to supplement the place-based wellbeing literature. How they negotiate their wellbeing remains to be observed in the daily home-making practices related to their two homes. This study conducted in-depth interviews with 35 migrating grandparents and nine of their adult children and conducted extensive field notes in Shanghai from 2020 to 2022. Through thematic analysis, it finds that the migrating grandparents met a series of differences, challenges and tensions in the material, social and emotional home-making practices brought by the separation and rotation between their own and their children’s homes. It weakens their physical, social and mental wellbeing. However, they take some initiatives to overcome and relieve these tensions. Therefore, accompanied by sacrifices and negotiations, they also obtain sustained material, social and spiritual–emotional values to negotiate a suboptimal experience of wellbeing. This study contributes to the intersection of elderly wellbeing and home-making studies by revealing the complex and ongoing inter-relationships between migrating grandparents and home in the rotating lifestyle.
Age Increases the Risk of Mortality by Four-Fold in Patients with Emergent Paralytic Ileus: Hospital Length of Stay, Sex, Frailty, and Time to Operation as Other Risk Factors
Authors: Elgar Guy , Smiley Parsa , Smiley Abbas , Feingold Cailan , Latifi Rifat
Published: August 2022

Background: In the United States, ileus accounts for USD 750 million of healthcare expenditures annually and significantly contributes to morbidity and mortality. Despite its significance, the complete picture of mortality risk factors for these patients have yet to be fully elucidated; therefore, the aim of this study is to identify mortality risk factors in patients emergently admitted with paralytic ileus. Methods: Adult and elderly patients emergently admitted with paralytic ileus between 2005–2014 were investigated using the National Inpatient Sample Database. Clinical outcomes, therapeutic management, demographics and comorbidities were collected. Associations between mortality and all other variables were established via univariable and multivariable logistic regression models. Results: A total of 81,674 patients were included, of which 45.2% were adults, 54.8% elderly patients, 45.8% male and 54.2% female. The average adult and elderly ages were 48.3 and 78.8 years, respectively. Elderly patients displayed a significantly (p < 0.01) higher mortality rate (3.0%) than adults (0.7%). The final multivariable logistic regression model showed that for every one-day delay in operation, the odds of mortality for adult and elderly patients increased by 4.1% (p = 0.002) and 3.2% (p = 0.014), respectively. Every additional year of age corresponded to 3.8% and 2.6% increases in mortality for operatively managed adult (p = 0.026) and elderly (p = 0.015) patients. Similarly, non-operatively treated adult and elderly patients displayed associations between mortality and advanced age (p = 0.001). The modified frailty index exhibited associations with mortality in operatively treated adults, conservatively managed adults and conservatively managed elderly patients (p = 0.001). Every additional day of hospitalization increased the odds of mortality in non-operative adult and elderly patients by 7.6% and 5.8%, respectively. Female sex correlated to lower mortality rates in non-operatively managed adult patients (odds ratio = 0.71, p = 0.028). Undergoing invasive diagnostic procedures in non-operatively managed elderly patients related to reduced mortality (odds ratio = 0.78, p = 0.026). Conclusions: Patients emergently admitted for paralytic ileus with increased hospital length of stay, longer time to operation, advanced age or higher modified frailty index displayed higher mortality rates. Female sex and invasive diagnostic procedures were negatively correlated with death in nonoperatively managed patients with paralytic ileus.
A Novel Homozygous Variant in DYSF Gene Is Associated with Autosomal Recessive Limb Girdle Muscular Dystrophy R2/2B
Authors: Spadafora Patrizia , Qualtieri Antonio , Cavalcanti Francesca , Di Palma Gemma , Gallo Olivier , De Benedittis Selene , Cerantonio Annamaria , Citrigno Luigi
Published: August 2022

Mutations in the DYSF gene, encoding dysferlin, are responsible for Limb Girdle Muscular Dystrophy type R2/2B (LGMDR2/2B), Miyoshi myopathy (MM), and Distal Myopathy with Anterior Tibialis onset (MDAT). The size of the gene and the reported inter and intra familial phenotypic variability make early diagnosis difficult. Genetic analysis was conducted using Next Gene Sequencing (NGS), with a panel of 40 Muscular Dystrophies associated genes we designed. In the present study, we report a new missense variant c.5033G>A, p.Cys1678Tyr (NM_003494) in the exon 45 of DYSF gene related to Limb Girdle Muscular Dystrophy type R2/2B in a 57-year-old patient affected with LGMD from a consanguineous family of south Italy. Both healthy parents carried this variant in heterozygosity. Genetic analysis extended to two moderately affected sisters of the proband, showed the presence of the variant c.5033G>A in both in homozygosity. These data indicate a probable pathological role of the variant c.5033G>A never reported before in the onset of LGMDR2/2B, pointing at the NGS as powerful tool for identifying LGMD subtypes. Moreover, the collection and the networking of genetic data will increase power of genetic-molecular investigation, the management of at-risk individuals, the development of new therapeutic targets and a personalized medicine.
Iron Source and Medium pH Affect Nutrient Uptake and Pigment Content in Petunia hybrida ‘Madness Red’ Cultured In Vitro
Authors: Guo Ge , Xiao Jie , Jeong Byoung Ryong
Published: August 2022

Deficiency or excess of iron (Fe) and improper medium pH will inhibit the growth and development of plants, reduce the transfer and utilization of energy from the root to the leaf, and affect the utilization efficiency of inorganic nutrients. The most common symptom of Fe deficiency in plants is chlorosis of the young leaves. In this study, the effects of the iron source, in combination with the medium pH, on plant growth and development, plant pigment synthesis, and nutrient uptake in a model plant Petunia hybrida cultured in vitro were investigated. Iron sulfate (FeSO4·7H2O) or iron chelated with ethylenediaminetetraacetic acid (Fe-EDTA) were supplemented to the MNS (a multipurpose nutrient solution) medium at a concentration of 2.78 mg·L−1 Fe, and the treatment without any Fe was used as the control. The pH of the agar-solidified medium was adjusted to either 4.70, 5.70, or 6.70 before autoclaving. The experiment was carried out in an environmentally controlled culture room with a temperature of 24 °C with 100 µmol·m−2·s−1 photosynthetic photon flux density (PPFD) supplied by white light emitting diodes (LEDs) during a photoperiod of 16 h a day, 18 °C for 8 h a day in the dark, and 70% relative humidity. Regardless of the Fe source including the control, the greatest number of leaves was observed at pH 4.70. However, the greatest lengths of the leaf and root were observed in the treatment with Fe-EDTA combined with pH 5.70. The contents of the chlorophyll, carotenoid, and anthocyanin decreased with increasing medium pH, and contents of these plant pigments were positively correlated with the leaf color. The highest soluble protein content and activities of APX and CAT were observed in the Fe-EDTA under pH 5.70. However, the GPX activity was the highest in the control under pH 4.70. In addition, the highest contents of ammonium (NH4+) and nitrate (NO3−) were measured in the FeSO4-4.7 and EDTA-5.7, respectively. More than that, the treatment of Fe-EDTA combined with pH 5.70 (EDTA-5.7) enhanced nutrient absorption, as proven by the highest tissue contents of P, K, Ca, Mg, Fe, and Mn. The genes’ ferric reduction oxidase 1 and 8 (PhFRO1 and PhFRO8), iron-regulated transporter 1 (PhIRT1), nitrate transporter 2.5 (PhNRT2.5), and deoxyhypusine synthase (PhDHS) were expressed at the highest levels in this treatment as well. In the treatment of EDTA-5.7, the reduction and transport of chelated iron in P. hybrida leaves were enhanced, which also affected the transport of nitrate and catalyzed chlorophyll level in leaves. In conclusion, when the medium pH was adjusted to 5.70, supplementation of chelated Fe-EDTA was more conducive to promoting the growth and development of, and absorption of mineral nutrients by, the plant and the expression of related genes in the leaves.
The Expression and Function of lincRNA-154324 and the Adjoining Protein-Coding Gene vmp1 in the Caudal Fin Regeneration of Zebrafish
Authors: Li Jing , Wen Wenjun , Zhang Shuqiang , Zhou Chune , Feng Yiyi , Li Xiaoyu
Published: August 2022

Caudal fin regeneration is regulated by a variety of mechanisms, but the role of long non-coding RNA (lncRNA) has rarely been studied. The present study aimed to describe the landscape of lncRNAs during caudal fin regeneration using whole transcriptome sequencing, and then to conduct a functional study on the target lncRNAs using real-time fluorescent quantitative PCR (RT-qPCR), in situ hybridization, and the CRISPR/Cas9 method for lncRNA gene knockout. The results of the transcriptome sequencing showed that a total of 381 lncRNAs were differentially expressed, among which ENSDART00000154324 (lincRNA-154324) was found to be highly related to caudal fin regeneration, and thus it was chosen as the target lncRNA for the subsequent functional study. The results regarding the temporal and spatial expression of lincRNA-154324 and the gene knockout results from CRISPR/Cas9 indicated that lincRNA-154324 is involved in the caudal fin regeneration of zebrafish. Importantly, we serendipitously discovered that the cis correlation coefficient between lincRNA-154324 and its neighboring gene vacuole membrane protein 1 (vmp1) is extremely high, and they are essential for the process of caudal fin regeneration. Moreover, studies have found that vmp1 plays an important role in protein secretion, organelle formation, multicellular development, and autophagy. Collectively, our result may provide a framework for the identification and analysis of lncRNAs involved in the regeneration of the zebrafish caudal fin.