SARS-CoV-2 viral titer measurements in Ontario, Canada wastewaters throughout the COVID-19 pandemic
Authors: D’Aoust Patrick M. , Hegazy Nada , Ramsay Nathan T. , Yang Minqing Ivy , Dhiyebi Hadi A. , Edwards Elizabeth , Servos Mark R. , Ybazeta Gustavo , Habash Marc , Goodridge Lawrence , Poon Art , Arts Eric , Brown R. Stephen , Payne Sarah Jane , Kirkwood Andrea , Simmons Denina , Desaulniers Jean-Paul , Ormeci Banu , Kyle Christopher , Bulir David , Charles Trevor , McKay R. Michael , Gilbride Kimberley , Oswald Claire , Peng Hui , Pileggi Vince , Wang Menglu L. , Tong Arthur , Orellano Diego ,   WSI Consortium, Adebiyi Adebowale , Advani Matthew , Agboola Simininuoluwa , Andino Dania , Aqeel Hussain , Badlani Yash , Bitter Lena Carolin , Bragg Leslie , Brasset-Gorny Julia , Breadner Patrick , Brown Stephen , Chan Ronny , Channa Babneet , Chen JinJin , Corchis-Scott Ryland , Cranney Matthew , Dang Hoang , Danna Nora , Dawe Rachel , DeGroot Christopher , de Melo Tomas , Dhiyebi Hadi , Donovan Justin , Eid Walaa , Ellmen Isaac , Farah Joud Abu , Farahbakhsh Farnaz , Fuzzen Meghan , Garant Tim , Geng Qiudi , Gedge Ashley , Gere Alice , Gibson Richard , Gilbride Kimberly , Goitom Eyerusalem , Gong Qinyuan , Graber Tyson , Hamilton Amanda , Haskell Blake , Hayat Samina , Ho Hannifer , Hungwe Yemurayi , Ikert Heather , Islam Golam , Joseph Dilan , Khan Ismail , Kibbee Richard , Knapp Jennifer , Knockleby James , Kwon Su-Hyun , Lawal Opeyemi U. , Lomheim Line , McKay Robert Michael , Menon Ria , Mercier Élisabeth , Miller Zach , Mloszewska Aleksandra M. , Mohammadiankia Ataollah , Naik Shiv , Nash Delaney , Ng Anthony , Olabode Abayomi , Örmeci Banu , Overton Alyssa , Pabon Gabriela Jimenez , Paramananthasivam Vinthiya , Pardy Jessica , Parreira Valeria R. , Pisharody Lakshmi , Prasla Samran , Precious Melinda , Rizvi Fozia , Santilli Matthew , Sarvi Hooman , Servos Mark , Siemon Dan , Sing-Judge Carly , Srikanthan Nivetha , Stephenson Sean , Sun Jianxian , Susilawati Endang , Tehrani Amir , Thakali Ocean , Wan Shen , Wellman Martin , Williams Katie , Yang Ivy , Zeeb Eli , Renouf Elizabeth M. , DeGroot Christopher T. , Delatolla Robert 
Published: June 2024

During the COVID-19 pandemic, the Province of Ontario, Canada, launched a wastewater surveillance program to monitor SARS-CoV-2, inspired by the early work and successful forecasts of COVID-19 waves in the city of Ottawa, Ontario. This manuscript presents a dataset from January 1, 2021, to March 31, 2023, with RT-qPCR results for SARS-CoV-2 genes and PMMoV from 107 sites across all 34 public health units in Ontario, covering 72% of the province’s and 26.2% of Canada’s population. Sampling occurred 2–7 times weekly, including geographical coordinates, serviced populations, physico-chemical water characteristics, and flowrates. In doing so, this manuscript ensures data availability and metadata preservation to support future research and epidemic preparedness through detailed analyses and modeling. The dataset has been crucial for public health in tracking disease locally, especially with the rise of the Omicron variant and the decline in clinical testing, highlighting wastewater-based surveillance’s role in estimating disease incidence in Ontario.