bluuebunny/crossref_metadata_2025 huggingface dataset:
Victim Sensitivity and Proposal Size Modulate the Ingroup Favoritism During Fairness Norm Enforcement
Authors: Zhang Zhen , Zhao Hui , Liu Ruixue , Qi Chunhui
Published: September 2021

People show a strong aversion to inequality and are willing to sacrifice their own interests to punish violations of fairness norms. Empirical research has found that group membership could influence the fairness judgment and norm enforcement of the individuals but has shown inconsistent findings and has not focused much on the potential moderators. Here, the two studies aimed to investigate whether victim sensitivity and proposal size moderate the impact of group membership on reactions to unfair proposals. In both studies, the participants with different victim sensitivity (low vs. high group) played the hypothetical (Study 1) and incentivized (Study 2) ultimatum game under the intragroup and intergroup condition and indicated their responses to the different proposals. Results showed that, regardless of the victim sensitivity, ingroup member is often given preferential and positive treatment. Low victim sensitive persons are more likely to accept unfair offers from the ingroup than the outgroup, while this effect was attenuated for those with high victim sensitivity, especially for highly ambiguous unfair offers (offer 6:4 in Study 1 and 8:2 in Study 2). Moreover, the ingroup favoritism score for ambiguous unfair offers was smaller for high compared with the victim sensitivity group. Taken together, the victim sensitivity, and proposal size could moderate the ingroup favoritism on responses to unfairness.
EFL Teachers' Apprehension and L2 Students' Classroom Engagement
Authors: Li Xiaodong
Published: September 2021

Teachers' apprehension is an indispensable part of the educational context since it impacts the amount to which students are engaged in class activities. Although several studies have been carried out considering the role of students' stress in their engagement, it seems extremely vital to conduct such studies among teachers to measure the link between these two variables. In this study, the author has made endeavors to define one of the apprehension's categories named communication apprehension and the antecedents of teachers' apprehension. Then classroom engagement is discussed. Following that, the relevance between the two variables of this research is discussed. Finally, both implications and suggestions for further studies are dealt with.
Electroencephalography in Assessment of Autism Spectrum Disorders: A Review
Authors: Milovanovic Maja , Grujicic Roberto
Published: September 2021

Electroencephalography (EEG) can further out our understanding of autistic spectrum disorders (ASD) neurophysiology. Epilepsy and ASD comorbidity range between 5 and 46%, but its temporal relationship, causal mechanisms and interplay with intellectual disability are still unknown. Epileptiform discharges with or without seizures go as high as 60%, and associate with epileptic encephalopathies, conceptual term suggesting that epileptic activity can lead to cognitive and behavioral impairment beyond the underlying pathology. Seizures and ASD may be the result of similar mechanisms, such as abnormalities in GABAergic fibers or GABA receptor function. Epilepsy and ASD are caused by a number of genetic disorders and variations that induce such dysregulation. Similarly, initial epilepsy may influence synaptic plasticity and cortical connection, predisposing a growing brain to cognitive delays and behavioral abnormalities. The quantitative EEG techniques could be a useful tool in detecting and possibly measuring dysfunctions in specific brain regions and neuronal regulation in ASD. Power spectra analysis reveals a U-shaped pattern of power abnormalities, with excess power in the low and high frequency bands. These might be the consequence of a complicated network of neurochemical changes affecting the inhibitory GABAergic interneurons and their regulation of excitatory activity in pyramidal cells. EEG coherence studies of functional connectivity found general local over-connectivity and long-range under-connectivity between different brain areas. GABAergic interneuron growth and connections are presumably impaired in the prefrontal and temporal cortices in ASD, which is important for excitatory/inhibitory balance. Recent advances in quantitative EEG data analysis and well-known epilepsy ASD co-morbidity consistently indicate a role of aberrant GABAergic transmission that has consequences on neuronal organization and connectivity especially in the frontal cortex.
Defining the Dynamic Regulation of O-GlcNAc Proteome in the Mouse Cortex---the O-GlcNAcylation of Synaptic and Trafficking Proteins Related to Neurodegenerative Diseases
Authors: Huynh Van N , Wang Sheng , Ouyang Xiaosen , Wani Willayat Y , Johnson Michelle S , Chacko Balu K , Jegga Anil G , Qian Wei-Jun , Chatham John C , Darley-Usmar Victor M , Zhang Jianhua
Published: September 2021

O-linked conjugation of ß-N-acetyl-glucosamine (O-GlcNAc) to serine and threonine residues is a post-translational modification process that senses nutrient availability and cellular stress and regulates diverse biological processes that are involved in neurodegenerative diseases and provide potential targets for therapeutics development. However, very little is known of the networks involved in the brain that are responsive to changes in the O-GlcNAc proteome. Pharmacological increase of protein O-GlcNAcylation by Thiamet G (TG) has been shown to decrease tau phosphorylation and neurotoxicity, and proposed as a therapy in Alzheimer’s disease (AD). However, acute TG exposure impairs learning and memory, and protein O-GlcNAcylation is increased in the aging rat brain and in Parkinson’s disease (PD) brains. To define the cortical O-GlcNAc proteome that responds to TG, we injected young adult mice with either saline or TG and performed mass spectrometry analysis for detection of O-GlcNAcylated peptides. This approach identified 506 unique peptides corresponding to 278 proteins that are O-GlcNAcylated. Of the 506 unique peptides, 85 peptides are elevated by > 1.5 fold in O-GlcNAcylation levels in response to TG. Using pathway analyses, we found TG-dependent enrichment of O-GlcNAcylated synaptic proteins, trafficking, Notch/Wnt signaling, HDAC signaling, and circadian clock proteins. Significant changes in the O-GlcNAcylation of DNAJC6/AUXI, and PICALM, proteins that are risk factors for PD and/or AD respectively, were detected. We compared our study with two key prior O-GlcNAc proteome studies using mouse cerebral tissue and human AD brains. Among those identified to be increased by TG, 15 are also identified to be increased in human AD brains compared to control, including those involved in cytoskeleton, autophagy, chromatin organization and mitochondrial dysfunction. These studies provide insights regarding neurodegenerative diseases therapeutic targets.
Universal Transceivers: Opportunities and Future Directions for the Internet of Everything (IoE)
Authors: Civas Meltem , Cetinkaya Oktay , Kuscu Murat , Akan Ozgur B.
Published: September 2021

The Internet of Everything (IoE) is a recently introduced information and communication technology (ICT) framework promising for extending the human connectivity to the entire universe, which itself can be regarded as a natural IoE, an interconnected network of everything we perceive. The countless number of opportunities that can be enabled by IoE through a blend of heterogeneous ICT technologies across different scales and environments and a seamless interface with the natural IoE impose several fundamental challenges, such as interoperability, ubiquitous connectivity, energy efficiency, and miniaturization. The key to address these challenges is to advance our communication technology to match the multi-scale, multi-modal, and dynamic features of the natural IoE. To this end, we introduce a new communication device concept, namely the universal IoE transceiver, that encompasses transceiver architectures that are characterized by multi-modality in communication (with modalities such as molecular, RF/THz, optical and acoustic) and in energy harvesting (with modalities such as mechanical, solar, biochemical), modularity, tunability, and scalability. Focusing on these fundamental traits, we provide an overview of the opportunities that can be opened up by micro/nanoscale universal transceiver architectures towards realizing the IoE applications. We also discuss the most pressing challenges in implementing such transceivers and briefly review the open research directions. Our discussion is particularly focused on the opportunities and challenges pertaining to the IoE physical layer, which can enable the efficient and effective design of higher-level techniques. We believe that such universal transceivers can pave the way for seamless connection and communication with the universe at a deeper level and pioneer the construction of the forthcoming IoE landscape.

Index Terms– Internet of Everything, Universal IoE Transceiver, Interoperability, Multi-modality, Hybrid Energy Harvesting, Molecular Communications, THz Communications, Graphene and related nanomaterials.
Age is Nothing but a Number: Ben 10s, Sugar Mummies, and the South African Gender Order in the Daily Sun’s Facebook Page
Authors: Boshoff Priscilla , Mlangeni Ntombi Lina
Published: September 2021

Stories about “Ben10” relationships between older women and their younger male lovers appear regularly in the Daily Sun, South Africa’s most popular tabloid newspaper. Daily Sun readers, who are typically township residents, engage vociferously over the rights and wrongs of such relationships on the tabloid’s Facebook page, and alternatively berate or support the older, working class women who feature in them. These women could be understood as “postfeminist” insofar as they are financially independent and sexually autonomous. Their actions echo those of the independent township women in the mid 20th century who, resisting patriarchal apartheid social engineering, brewed beer and rented rooms in order to assert their financial and sexual independence. In both cases, these women’s bold actions confront local hetero-patriarchal norms and call into question an ideal local patriarchal gender order. However, the meanings that are made by the readers of such women in Ben10 relationships today also reflect a social context characterised by a contestation over the meaning of rights, high rates of unemployment, gender-based violence and HIV, factors that curtail a premature diagnosis of postfeminist identity. Drawing on a textual analysis of several articles and their Facebook comments, we argue that any assessment of postfeminism in southern spaces must account for how historical and contextual factors such as these constrain the reach of global postfeminism.
Robotic Cochlear Implant Surgery: Imaging-Based Evaluation of Feasibility in Clinical Routine
Authors: Auinger Alice Barbara , Dahm Valerie , Liepins Rudolfs , Riss Dominik , Baumgartner Wolf-Dieter , Arnoldner Christoph
Published: September 2021

Background: Robotic surgery has been proposed in various surgical fields to reduce recovery time, scarring, and to improve patients' outcomes. Such innovations are ever-growing and have now reached the field of cochlear implantation. To implement robotic ear surgery in routine, it is of interest if preoperative planning of a safe trajectory to the middle ear is possible with clinically available image data.

Methods: We evaluated the feasibility of robotic cochlear implant surgery in 50 patients (100 ears) scheduled for routine cochlear implant procedures based on clinically available imaging. The primary objective was to assess if available high-resolution computed tomography or cone beam tomography imaging is sufficient for planning a trajectory by an otological software. Secondary objectives were to assess the feasibility of cochlear implant surgery with a drill bit diameter of 1.8 mm, which is the currently used as a standard drill bit. Furthermore, it was evaluated if feasibility of robotic surgery could be increased when using smaller drill bit sizes. Cochlear and trajectory parameters of successfully planned ears were collected. Measurements were carried out by two observers and the interrater reliability was assessed using Cohen's Kappa.

Results: Under the prerequisite of the available image data being sufficient for the planning of the procedure, up to two thirds of ears were eligible for robotic cochlear implant surgery with the standard drill bit size of 1.8 mm. The main reason for inability to plan the keyhole access was insufficient image resolution causing anatomical landmarks not being accurately identified. Although currently not applicable in robotic cochlear implantation, narrower drill bit sizes ranging from 1.0 to 1.7 mm in diameter could increase feasibility up to 100%. The interrater agreement between the two observers was good for this data set.

Discussion: For robotic cochlear implant surgery, imaging with sufficient resolution is essential for preoperative assessment. A slice thickness of <0.3 mm is necessary for trajectory planning. This can be achieved by using digital volume tomography while radiation exposure can be kept to a minimum. Furthermore, surgeons who use the software tool, should be trained on a regular basis in order to achieve planning consistency.
Seasonally Related Disruption of Metabolism by Environmental Contaminants in Male Goldfish (Carassius auratus)
Authors: Bottalico Lisa N. , Korlyakova Julia , Weljie Aalim M. , Habibi Hamid R
Published: September 2021

Endocrine disrupting chemicals mimic or disrupt action of the natural hormones, adversely impacting hormonal function as well as cardiovascular, reproductive, and metabolic health. Goldfish are seasonal breeders with an annual reproductive cycle regulated by neuroendocrine signaling which involves allocation of metabolic energy to sustain growth and reproduction. We hypothesize that seasonal changes in physiology alter overall vulnerability of goldfish to metabolic perturbation induced by environmental contaminants. In this study, we assess effects of endogenous hormones, individual contaminants and their mixture on metabolism of goldfish at different reproductive stages. Exposure effects were assessed using 1H-NMR metabolomics profiling of male goldfish midbrain, gonad and liver harvested during early recrudescence (October), mid-recrudescence (February) and late recrudescence (June). Compounds assessed include bisphenol A, nonylphenol, bis(2-ethylhexyl) phthalate, fucosterol and a tertiary mixture (DEHP + NP + FS). Metabolome-level responses induced by contaminant exposure across tissues and seasons were benchmarked against responses induced by 17β-estradiol, testosterone and thyroid hormone (T3). We observe a clear seasonal dependence to metabolome-level alteration induced by hormone or contaminant exposures, with February (mid-recrudescence) the stage at which male goldfish are most vulnerable to metabolic perturbation. Responses induced by contaminant exposures differed from those induced by the natural hormones in a season-specific manner. Exposure to the tertiary mixture induced a functional gain at the level of biochemical pathways modeling over responses induced by individual components in select tissues and seasons. We demonstrate the importance of seasonally driven changes in physiology altering overall vulnerability of goldfish to metabolic perturbation induced by environmental contaminants, the relevance of which likely extends to other seasonally-breeding species.
Predicting Parasite Dynamics in Mixed-Use Trans-Himalayan Pastures to Underpin Management of Cross-Transmission Between Livestock and Bharal
Authors: Khanyari Munib , Suryawanshi Kulbhushansingh R. , Milner-Gulland E. J. , Dickinson Eleanor , Khara Abhirup , Rana Rashmi Singh , Rose Vineer Hannah , Morgan Eric R.
Published: September 2021

The complexities of multi-use landscapes require sophisticated approaches to addressing disease transmission risks. We explored gastro-intestinal nematode (GINs) infections in the North India Trans-Himalayas through a socio-ecological lens, integrating parasite transmission modelling with field surveys and local knowledge, and evaluated the likely effectiveness of potential interventions. Bharal (blue sheep;Pseudois nayaur), a native wild herbivore, and livestock share pasture year-round and livestock commonly show signs of GINs infection. While both wild and domestic ungulates had GINs infections, egg counts indicated significantly higher parasite burdens in bharal than livestock. However, due to higher livestock densities, they contributed more to the total count of eggs and infective larvae on pasture. Herders also reported health issues in their sheep and goats consistent with parasite infections. Model simulations suggested that pasture infectivity in this system is governed by historical pasture use and gradually accumulated larval development during the summer, with no distinct short-term flashpoints for transmission. The most effective intervention was consequently predicted to be early-season parasite suppression in livestock using temperature in spring as a cue. A 1-month pause in egg output from livestock could lead to a reduction in total annual availability of infective larvae on pasture of 76%, potentially benefitting the health of both livestock and bharal. Modelling suggested that climate change over the past 33 years has led to no overall change in GINs transmission potential, but an increase in the relative influence of temperature over precipitation in driving pasture infectivity. Our study provides a transferable multi-pronged approach to investigating disease transmission, in order to support herders' livelihoods and conserve wild ungulates.
Productive Performance, Carcass Traits, and Meat Quality in Finishing Lambs Supplemented with a Polyherbal Mixture
Authors: Orzuna-Orzuna José Felipe , Dorantes-Iturbide Griselda , Lara-Bueno Alejandro , Mendoza-Martínez Germán David , Miranda-Romero Luis Alberto , López-Ordaz Rufino , Hernández-García Pedro Abel
Published: September 2021

The objective of this study was to evaluate the effects of dietary supplementation of a polyherbal mixture (HM) containing saponins, flavonoids, and polysaccharides on productive performance, carcass characteristics and meat quality of lambs during the final fattening period. Thirty-six Dorper × Katahdin lambs (23.27 ± 1.23 kg body weight (BW)) were housed in individual pens and were assigned to four treatments (n = 9) with different doses of HM: 0 (CON), 1 (HM1), 2 (HM2) and 3 (HM3) g of HM kg−1 of DM for 56 days. Data were analysed as a completely randomized design using the MIXED and GLM procedures of statistical analysis system (SAS), and linear and quadratic effects were tested to evaluate the effects of the HM level. DM digestibility decreased in lambs fed HM3 (p < 0.05). There was no effect of HM on daily weight gain, dry matter intake, final BW, feed conversion, carcass characteristics, colour (L* and a*) and meat chemical composition. Meat pH, cooking loss and drip loss increased linearly (p < 0.05) when the HM dose was increased. The Warner-Bratzler shear force (WBSF) of meat was lower (p < 0.05) in lambs fed HM3. In conclusion, dietary inclusion of 3 g HM kg1 of DM improves meat tenderness. However, high doses of HM in the diet may decrease the digestibility of DM and increase the cooking loss and drip loss of lamb meat during the final fattening period.
Mushroom Phorid Flies—A Review
Authors: Navarro María Jesús , Escudero-Colomar Lucía Adriana , Carrasco Jaime , Gea Francisco José
Published: September 2021

Diptera are among the most serious arthropod pests affecting mushroom crops. Phorid flies, especially Megaselia halterata, have traditionally been globally considered as a minor pest, although they are a very important problem on Spanish mushroom farms. The concerns with respect to the phorid fly populations have recently increased, notably jumping from being a minor to major pest in India, UK and the USA, where yield losses ranging between 10% and 40% were reported. This review updates and summarizes the available literature regarding mushroom phorid populations, stressing the natural distribution of phorids and their seasonal distribution, their biology within the growing substrates and the initial sources of infestation on mushroom farms. Moreover, the review also highlights the scarce available tools for their control and the current alternatives to chemical products.
Improving Soil Moisture Assessment of Turfgrass Systems Utilizing Field Radiometry
Authors: Roberson Travis L. , Badzmierowski Mike J. , Stewart Ryan D. , Ervin Erik H. , Askew Shawn D. , McCall David S.
Published: September 2021

The need for water conservation continues to increase as global freshwater resources dwindle. Turfgrass mangers are adapting to these concerns by implementing new tools to reduce water consumption. Time-domain reflectometer (TDR) soil moisture sensors can decrease water usage when scheduling irrigation, but nonuniformity across unsampled locations creates irrigation inefficiencies. Remote sensing data have been used to estimate soil moisture stress in turfgrass systems through the normalized difference vegetation index (NDVI). However, numerous stressors other than moisture constraints impact NDVI values. The water band index (WBI) is an alternative index that uses narrowband, near-infrared light reflectance to estimate moisture limitations within the plant canopy. The green-to-red ratio index (GRI) is a vegetation index that has been proposed as a cheaper alternative to WBI as it can be measured using digital values of visible light instead of relying on more costly hyperspectral reflectance measurements. A replicated 2 × 3 factorial experimental design was used to repeatedly measure turf canopy reflectance and soil moisture over time as soils dried. Pots of ‘007’ creeping bentgrass (CBG) and ‘Latitude 36’ hybrid bermudagrass (HBG) were grown on three soil textures: United States Golf Association (USGA) 90:10 sand, loam, and clay. Reflectance data were collected hourly between 07:00 and 19:00 using a hyperspectral radiometer and volumetric water content (VWC) data were collected continuously using an embedded soil moisture sensor from soil saturation until complete turf necrosis by drought stress. The WBI had the strongest relationship to VWC (r = 0.62) compared to GRI (r = 0.56) and NDVI (r = 0.47). The WBI and GRI identified significant moisture stress approximately 28 h earlier than NDVI (p = 0.0010). Those metrics also predicted moisture stress prior to fifty percent visual estimation of wilt (p = 0.0317), with lead times of 12 h (WBI) and 9 h (GRI). By contrast, NDVI provided 2 h of prediction time. Nonlinear regression analysis showed that WBI and GRI can be useful for predicting moisture stress of CBG and HBG grown on three different soil textures in a controlled environment.
Optimizing Peach Tree Canopy Architecture for Efficient Light Use, Increased Productivity and Improved Fruit Quality
Authors: Anthony Brendon M. , Minas Ioannis S.
Published: September 2021

Peach production in the USA has been in decline in recent decades due to poor fruit quality, reduced consumption and increased cost of production. Productivity and fruit quality can only be enhanced in the orchard through optimizing preharvest factors such as orchard design and training systems. Transition from low-density plantings (LDP) to high-density plantings (HDP) in peach is associated with the availability of reliable size controlling rootstocks. Increased densities must be combined with modern training systems to diffuse vigor and further increase light interception and yields, while optimizing light distribution, fruit quality and cost of production. Several training systems have been tested in peach with various objectives and goals, such as increasing light, water use and labor efficiencies, along with designing canopy architectures to facilitate mechanization and robotics. In general, increased planting densities increase yields, but excessive densities can promote shade, while excessive crop load can deteriorate quality. An ideal peach cropping system should optimize light interception and light distribution to balance maximum yield potential with maximum fruit quality potential. Successful management of high-density peach fruiting wall systems can lead to enhanced and uniform fruit quality, and ensure a sustainable industry.
Effect of Opuntia ficus-indica Mucilage Edible Coating on Quality, Nutraceutical, and Sensorial Parameters of Minimally Processed Cactus Pear Fruits
Authors: Liguori Giorgia , Gaglio Raimondo , Greco Giuseppe , Gentile Carla , Settanni Luca , Inglese Paolo
Published: September 2021

Cactus pear (Opuntia ficus-indica (L.) Mill.) is a non-climacteric fruit with a relatively short postharvest life span, being very sensitive to water loss, darkening and decay. Cactus pear is a spiny fruit, and the presence of glochids limits fruit consumption and diffusion; therefore, minimally processing, as well as peel removing, could be an opportunity to improve its availability, consumption, and diffusion in national and international markets. In this study, cactus pear minimally processed fruits were treated with a mucilage-based coating extracted from Opuntia ficus-indica cladodes and stored at 5 °C for 9 days. The effect of mucilage edible coating on the postharvest life, qualitative attributes, and nutraceutical value of fruit were evaluated by colors, firmness, total soluble solids content, titratable acidity, ascorbic acid, betalains and DPPH (2,2-diphenyl-1-picrylhydrazyl). Results showed that mucilage-based coating improved the quality and preserves the nutraceutical value of minimally processed cactus pear fruits during storage. The edible coating was effective in maintaining fruit fresh weight, total soluble solids content, fruit firmness, ascorbic acid and betalain content, sensorial traits, and visual score. Coated fruits showed a significantly lower microbiological growth than uncoated control fruits during the entire cold storage period.
Soil Organic Carbon and System Environmental Footprint in Sugarcane-Based Cropping Systems Are Improved by Precision Land Leveling
Authors: Naresh Rama Krishna , Bhatt Rajan , Chandra M. Sharath , Laing Alison M. , Gaber Ahmed , Sayed Samy , Hossain Akbar
Published: September 2021

A six-year experiment (2009 to 2015) was conducted on sugarcane-based cropping systems in farmers’ fields to examine the effects of precision land leveling (PLL) compared to traditional land leveling (TLL) in terms of soil organic carbon (SOC), greenhouse gas emissions, irrigation water requirements, and system productivity and profitability. Twelve treatments compared different sugarcane sowing regimes and crops in rotation under both PLL and TLL. Spring-sown sugarcane grown in rotation with rice, potato, and wheat under PLL had the highest production (89.7 kg ha−1 day−1) and required 142 cm irrigation water, which was 35.1% less water than a commonly practiced cropping system with late-sown sugarcane grown in rotation with rice and wheat only under TLL). Cropping systems established under PLL had higher land use efficiency (ranging between 64.9 and 86.2%), higher energy productivity (90.7 to 198.6 GJ ha−1), and lower greenhouse gas emissions (5249.33 to 944.19 kg CO2 eq ha−1 year−1) than those under TLL. As well, treatments under PLL had increased levels of SOC, particularly in the upper soil layers, relative to SOC in treatments under TLL. Combining PLL with diversification of crops in sugarcane cropping systems has the potential to sustainably increase farmers’ land productivity and profitability while improving soil health and reducing irrigation requirements. These benefits are likely to have applications in other sugarcane-based cropping systems in similar agro-ecologies.
Effects of Feeding Varying Levels of DL-Methionine on Live Performance and Yield of Broiler Chickens
Authors: Pokoo-Aikins Anthony , Timmons Jennifer Rumsey , Min Byungrok Rok , Lee William Robert , Mwangi Samuel Njoroge , Chen Chongxiao
Published: September 2021

This study was designed to evaluate the effects of dietary supplemental DL-methionine (MET) on live performance and meat yield for broilers raised to a common weight. A total of 1552 one-day old Ross 708, sexed broilers were randomly distributed to 32 pens resulting in eight treatments (TRT) of four replicates with 44 male or 53 female/pen. A randomized complete block with a 2 × 4 (sex × 4 MET levels 0, 0.5, 1, and 2 g/kg) factorial arrangement of TRT was used. A common weight of 2400 g was approached by day 46 (1 and 2 g MET/kg feed) and day 48 (0 and 0.5 g MET/kg feed). Supplementation of MET at 1, and 2 g/kg had a lower (p < 0.01) feed conversion ratio (FCR) at day 46/48 than broilers fed 0.5 g MET/kg. Broilers without supplemental MET had the worst (p < 0.01) feed conversion and average daily gain (ADG) at day 46/48. Birds fed 0 g MET/kg of feed had lower (p < 0.05) whole eviscerated carcass without giblets (WOG), yield than birds fed 2 g MET/kg of feed. Additionally, birds fed 0 g MET/kg of feed had lower (p < 0.05) breast fillet and tender percent yields than birds fed supplemental MET. Elimination of MET from organic broiler diets resulted in reduced ADG, breast fillet yield and feed efficiency of meat yield of broilers raised to day 46/48. Reduction in MET supplementation below current levels reduced the efficiency of meat production of organic broilers raised to day 46/48.
Antioxidant and Antiapoptotic Effects of a Turraea fischeri Leaf Extract on Cryopreserved Goat Sperm
Authors: Hassan Soha A. , Khalil Wael A. , Hassan Mahmoud A. E. , Yousif Ahmed I. , Sabry Omar M. , Wink Michael , Sobeh Mansour
Published: September 2021

This study evaluated the efficacy of Turraea fischeri leaf extract for maintaining the viability of cryopreserved goat sperm. Ejaculated semen was collected from 5 mature Baladi bucks (50–60 kg, 2–4 years of age) and those samples with mass motility ≥ 70% and sperm concentration ≥ 2.5 × 109/mL were selected, pooled, and divided into 4 aliquots. Each aliquot was diluted in Tris-citric-soybean lecithin extender containing a different concentration of T. fischeri leaf extract (0, 125, 250, or 375 µg/mL). Treated semen samples were cooled to 5 °C, transferred to 0.25-mL French straws, and stored in liquid nitrogen (LN2) at −196 °C. After thawing, membrane integrity was examined by transmission electron microscopy, apoptotic activity by Annexin/propidium iodide staining and flow cytometry, and both enzyme activities and antioxidant capacity by spectroscopic assays. The leaf extract at 375 µg/mL significantly improved semen quality as indicated by enhanced total antioxidant capacity, reduced H2O2 concentration, a greater proportion of structurally intact motile sperm, and concomitant reductions in apoptosis and necrosis. The extract also significantly increased the proportion of sperm with a contiguous plasma membrane and intact acrosome (p < 0.05). Furthermore, LC-MS revealed numerous secondary metabolites in the extract that may contribute to sperm cryopreservation.
Broiler Chicken Behavior and Activity Are Affected by Novel Flooring Treatments
Authors: Jacobs Leonie , Melick Shawnna , Freeman Nathan , Garmyn An , Tuyttens Frank A. M.
Published: September 2021

The objective was to determine broiler chicken behavioral differences in response to novel flooring treatments. Broilers (n = 182) were housed in 14 pens (a random subset from a larger-scale study including 42 pens), with 13 birds/pen. One of seven flooring treatments were randomly allocated to 14 pens (2 pens per treatment). The flooring treatments (provided from day 1 {1} or day 29 {29}) included regularly replaced shavings (POS), a mat with 1% povidone-iodine solution (MAT), and the iodine mat placed on a partially slatted floor (SLAT). In addition, a negative control treatment was included with birds kept on used litter from day 1 (NEG). Behavior was recorded in weeks 1, 2, 5, and 6. In week 5, treatments affected the behavioral repertoire (p ≤ 0.035). Birds in POS-1 showed more locomoting, preening and activity overall compared to MAT and/or SLAT treatments. Birds in POS-29 showed more drinking, foraging, preening and overall activity than birds in MAT and/or SLAT treatments. In week 6, birds in the POS-1 treatment spent more time foraging compared to birds in all MAT and SLAT treatments (p ≤ 0.030). In addition, birds in the POS-1 treatment spent more time preening than birds in the MAT-1 treatment (p = 0.046). Our results indicate that access to partially slatted flooring and/or disinfectant mats does not benefit broiler chicken welfare in terms of their ability to express highly motivated behaviors. Access to clean, regularly replaced litter is beneficial for broiler chicken welfare in terms of their ability to express their normal behavioral repertoire.
The Effect of Different Concentrations of Total Polyphenols from Paulownia Hybrid Leaves on Ruminal Fermentation, Methane Production and Microorganisms
Authors: Puchalska Julia , Szumacher-Strabel Małgorzata , Patra Amlan Kumar , Ślusarczyk Sylwester , Gao Min , Petrič Daniel , Nabzdyk Maria , Cieślak Adam
Published: September 2021

This experiment was conducted to study the effects of different concentrations of polyphenols of Paulownia Clon In Vitro 112® leaves or their particular parts on in vitro ruminal fermentation, methane production and microbial population. Paulownia leaves with high (PLH; 31.35 mg/g dry matter (DM)), medium (PLM; 26.94 mg/g DM), and low level of polyphenols (PLL; 11.90 mg/g DM) were used from three plantation areas. Lamina (PLLA; 33.63 mg/g DM) and twigs (PLT; 2.53 mg/g DM) of leaves were also collected from the PLM plantation. The chemical analyses of Paulownia leaves indicated that the content of the most basic nutrients (e.g., crude protein concentration of 185 g/kg of DM) were similar to dehydrated alfalfa. The in vitro results showed that the use of Paulownia leaves with the highest content of total polyphenols (PLH and PLLA) decreased methane production, methanogens numbers, and acetate to propionate ratio. In PLT, lowered methane production was followed by reduced substrate degradability and volatile fatty acid (VFA) concentration along with higher acetate to propionate ratio. Therefore, reduction of methane production in PLH and PLLA was attributed to the lowered methanogen population, whereas in PLT it was caused by decreased substrate degradability with the resultant of limited hydrogen availability to the methanogens.
Characterization and Comparison of Enterococcus spp. Isolates from Feces of Healthy Dogs and Urine of Dogs with UTIs
Authors: Stępień-Pyśniak Dagmara , Bertelloni Fabrizio , Dec Marta , Cagnoli Giulia , Pietras-Ożga Dorota , Urban-Chmiel Renata , Ebani Valentina Virginia
Published: September 2021

Enterococcus spp. are opportunistic pathogens of both humans and animals characterized by high resistance to antimicrobials. Dogs could be intestinal carriers or suffer from Enterococcus infections, mainly urinary tract infections (UTIs). This study aimed to analyze and compare Enterococcus spp. isolated from healthy dog stools and sick dog urine. Overall, 51 isolates (29 from stools and 22 from UTI) were characterized at species level and tested for antimicrobial resistance, biofilm production and presence of resistance and virulence genes. E. faecium and E. faecalis resulted as equally distributed in stools samples, while E. faecalis predominated among UTI isolates. HLAR phenotype was detected in 47.1% isolates; 64.7% isolates were resistant to ampicillin (47.1% with a MIC ≥ 64 µg/mL). High levels of resistance were recorded for fluoroquinolones (enrofloxacin 74.5%, ciprofloxacin 66.7%), clindamycin (84.3%), tetracycline (78.4%) and quinupristin–dalfopristin (78.4%). No vancomycin resistant strains were detected. All but one isolate were multidrug-resistant. Most detected resistance genes were tetM (70.5%), pbp4 (52.9%) and aph(3′)-IIIa (39.2%). All isolates were able to produce biofilm, but isolates from UTIs and belonging to E. faecalis more frequently resulted in strong biofilm producers. Most detected virulence genes were asa1 (52.9%), gelE (41.2%), cylA (37.3%) and esp (35.3%); all of them resulted as more frequently associated to E. faecalis. No particular differences emerged between isolates from feces and UTI, considering all evaluated aspects. Our results confirm pet dogs as carriers of multidrug-resistant enterococci; stool microflora could be considered as the most probable source of enterococcal UTI and E. faecalis carried by dogs seems to be more virulent than E. faecium, justifying its more frequent involvement in urinary tract infections.
Xarifiid Copepods (Copepoda: Cyclopoida: Xarifiidae) Parasitic in the Coral Psammocora columna Dana, 1846 from Taiwan
Authors: Cheng Yu-Rong , Lu Tsai-Ming , Ding De-Sing
Published: September 2021

A comprehensive knowledge of relationships between coral and coral-associated organisms is essential for the conservation studies of the coral reef community, yet the biodiversity database of coral-inhabiting copepods remains incomplete. Here we surveyed in a widely distributed scleractinian coral, Psammocora columna Dana, 1846, and newly discovered two endoparasitic copepod species, Xarifiayanliaoensis sp. nov. and Xarifia magnifica sp. nov. These two new species are described based on specimens collected in Taiwan, and they share several common morphological characters of Xarifia copepods, i.e., region dorsal to fifth legs having three posteriorly directed processes unequally. However, X. yanliaoensis sp. nov. is distinguishable from other species by the morphology of the endopods of legs, antenna, maxilla, and maxilliped (in both genders). The morphological characters of X. magnifica sp. nov. are the endopods of legs, leg 5, and maxilliped in the male. Including the two new species described in the present work, the genus Xarifia Humes, 1960 belongs to the cyclopoid family Xarifiidae Humes, 1960 currently consists of 94 species, and eight of them live in association with the Psammocora coral. A comparison table and a key to the species of Xarifia from Psammocora corals are given herein.
Imipenem/Relebactam Ex Vivo Clearance during Continuous Renal Replacement Therapy
Authors: Jang Soo Min , Yessayan Lenar , Dean Michael , Costello Gabrielle , Katwaru Ravi , Mueller Bruce A.
Published: September 2021

(1) Purpose of this study: determination of adsorption and transmembrane clearances (CLTM) of imipenem and relebactam in ex vivo continuous hemofiltration (CH) and continuous hemodialysis (CHD) models. These clearances were incorporated into a Monte Carlo Simulation (MCS), to develop drug dosing recommendations for critically ill patients requiring continuous renal replacement therapy (CRRT); (2) Methods: A validated ex vivo bovine blood CH and CHD model using two hemodiafilters. Imipenem/relebactam and urea CLTM at different ultrafiltrate/dialysate flow rates were evaluated in both CH and CHD. MCS was performed to determine dose recommendations for patients receiving CRRT; (3) Results: Neither imipenem nor relebactam adsorbed to the CRRT apparatus. The CLTM of imipenem, relebactam, and urea approximated the effluent rates (ultrafiltrate/dialysate flow rates). The types of hemodiafilter and effluent rates did not influence CLTM except in a dialysis flow rate of 1 L/h and 6 L/h in the CHD with relebactam (p < 0.05). Imipenem and relebactam 200 mg/100 mg every 6 h were sufficient to meet the standard time above the MIC pharmacodynamic targets in the modeled CRRT regimen of 25 kg/mL/h. (4) Conclusions: Imipenem and relebactam are not removed by adsorption to the CRRT apparatus, but readily cross the hemodiafilter membrane in CH and CHD. Dosage adjustment of imipenem/relebactam is likely required for critically ill patients receiving CRRT.
Plasma Nitrate Levels Are Related to Metabolic Syndrome and Are Not Altered by Treatment with DPP-4 Inhibitor Linagliptin: A Randomised, Placebo-Controlled Trial in Patients with Early Type 2 Diabetes Mellitus
Authors: Reijrink Melanie , De Boer Stefanie A. , Van Roon Anniek M. , Slart Riemer H. J. A. , Fernandez Bernadette O. , Feelisch Martin , Heerspink Hiddo J. L. , Van Goor Harry , Hillebrands Jan-Luuk , Mulder Douwe J.
Published: September 2021

The depletion of nitrate and nitrite, stable nitric oxide (NO) end-products, promotes adipose tissue dysfunction and insulin resistance (IR). Dipeptidyl peptidase-4 (DPP-4) inhibitors have the potentially beneficial side effect of increasing NO availability. In this study, nitrate and nitrite levels and the effects of DPP-4 inhibitor linagliptin were investigated in relation to metabolic syndrome (MetS) markers. Treatment-naive patients with early type 2 diabetes mellitus (T2DM) (n = 40, median age 63 IQR (55–67) years, 63% male, mean HbA1c 45 ± 4.4 mmol/mol) were randomized (1:1) to linagliptin (5 mg/day) or placebo. MetS-related markers (body mass index (BMI), triglycerides, HOMA-IR, gamma-glutamyltransferase (GGT), C-reactive protein (CRP), and adiponectin), plasma levels of nitrate, nitrite, total free thiols (TFT) and vegetable intake were estimated at baseline and after 4 and 26 weeks of treatment. Plasma nitrate, but not nitrite, correlated positively with vegetable intake (r = 0.38, p = 0.018) and was inversely associated with HOMA-IR (r = −0.44, p = 0.006), BMI (r = −0.35, p = 0.028), GGT (r = −0.37, p = 0.019) and CRP (r = −0.34, p = 0.034). The relationship between nitrate and HOMA-IR remained significant after adjusting for BMI, CRP, vegetable intake and GGT. With stable vegetable intake, nitrate and nitrite, TFT, adipokines and CRP did not change after 26 weeks of linagliptin treatment. While plasma nitrate is inversely associated with MetS, linagliptin treatment does not significantly influence nitrate and nitrite concentrations, oxidative stress, adipose tissue function and systemic inflammation.
Changes in Reactive Oxygen Species, Antioxidants and Carbohydrate Metabolism in Relation to Dormancy Transition and Bud Break in Apple (Malus × domestica Borkh) Cultivars
Authors: Sapkota Sangeeta , Liu Jianyang , Islam Md Tabibul , Sherif Sherif M.
Published: September 2021

Understanding the biochemical mechanisms underlying bud dormancy and bloom time regulation in deciduous woody perennials is critical for devising effective strategies to protect these species from spring frost damage. This study investigated the accumulation profiles of carbohydrates, ROS and antioxidants during dormancy in ‘Cripps Pink’ and ‘Honeycrisp’, two apple cultivars representing the early and late bloom cultivars, respectively. Our data showed that starch levels generally declined during dormancy, whereas soluble sugars increased. However, the present study did not record significant alternations in the carbohydrate accumulation profiles between the two cultivars that could account for the differences in their bloom dates. On the other hand, H2O2 accumulation patterns revealed an apparent correlation with the dormancy stage and bloom dates in both cultivars; peaking early in the early-blooming cultivar, sustaining high levels for a longer time in the late-blooming cultivars, and fading by the time of bud burst in both cultivars. Also, the redox balance during dormancy appeared to be maintained mainly by catalase and, to a lesser extent, by glutathione (GSH). Overall, the present study concludes that differences in ROS and the bud redox balance could, at least partially, explain the differences in dormancy duration and bloom date among apple cultivars.
Undrained Stability Analysis of Shallow Tunnel and Sinkhole in Soft Clay: The Cavity Contraction Method
Authors: Qi Hui , Cui Wenjie , Li Huaijian , Cheng Junwei , Kong Lingdi , Wang Xiaonan , Zhang Jianliang , Yang Gongzeng , Yue Hongya , Song Xiuguang
Published: September 2021

This paper presents theoretical methods for the undrained stability analysis of shallow tunnels/sinkholes in clay based on the cavity contraction theory, with some assumptions and simplifications. To examine the accuracy and reliability of the new methods, a database was assembled, which consists of stability numbers of tunnel/sinkholes in clays from 22 centrifuge model tests, 10 field tests, and 62 FELA results. It is shown that the proposed methods give an average of 2.5% overestimation for the stability numbers from model tests and is in a good agreement with the FELA results. The cavity contraction theory-based methods are then discussed, which could provide useful guidance for designers to roughly assess shallow tunnel/sinkhole stability in clays.
An Optimization Technique for Linear Manifold Learning-Based Dimensionality Reduction: Evaluations on Hyperspectral Images
Authors: Öztürk Ümit , Yılmaz Atınç
Published: September 2021

Manifold learning tries to find low-dimensional manifolds on high-dimensional data. It is useful to omit redundant data from input. Linear manifold learning algorithms have applicability for out-of-sample data, in which they are fast and practical especially for classification purposes. Locality preserving projection (LPP) and orthogonal locality preserving projection (OLPP) are two known linear manifold learning algorithms. In this study, scatter information of a distance matrix is used to construct a weight matrix with a supervised approach for the LPP and OLPP algorithms to improve classification accuracy rates. Low-dimensional data are classified with SVM and the results of the proposed method are compared with some other important existing linear manifold learning methods. Class-based enhancements and coefficients proposed for the formulization are reported visually. Furthermore, the change on weight matrices, band information, and correlation matrices with p-values are extracted and visualized to understand the effect of the proposed method. Experiments are conducted on hyperspectral imaging (HSI) with two different datasets. According to the experimental results, application of the proposed method with the LPP or OLPP algorithms outperformed traditional LPP, OLPP, neighborhood preserving embedding (NPE) and orthogonal neighborhood preserving embedding (ONPE) algorithms. Furthermore, the analytical findings on visualizations show consistency with obtained classification accuracy enhancements.
Synthesis, Spectroscopic Characterization and Biological Studies of Mn(II), Cu(II), Ni(II), Co(II) and Zn(II) Complexes with New Schiff Base of 2-((Pyrazine-2-ylimino)methyl)phenol
Authors: Howsaui Hanan B. , Sharfalddin Abeer A. , Abdellattif Magda H. , Basaleh Amal S. , Hussien Mostafa A.
Published: September 2021

In the search for novel anticancer complex, transition metal complexes of Schiff base derived from 2-aminopyrazine and salicylaldehyde were successfully synthesized and characterized based on elemental analyses, spectroscopic and thermal analysis. The IR spectra showed the ligand is a tridentate chelator with O, N and N atoms. donor sites in the Zn(II), Co(II), Ni(II), and Mn(II) complexes. Contrary, it behaved a bidentate chelator in the Cu complex by O and N. Molar ratio data revealed that the ligand to metal ratio was 1:2 for Co(II) Cu(II) and Zn(II) while it was 1:1 for Mn(II) and Ni(II) complexes. The obtained complexes have the formulae [M(L)2] (where M = Co(II) and Zn(II), [M(L)Cl(2H2O)]·H2O (where M = Ni(II) and Mn(II) were octahedral geometry). The computational studies were performed by DFT calculations to compare the optimized geometries with the experimental values. The interaction of these complexes with calf thymus DNA (CT-DNA) was investigated by UV-Vis spectroscopic technique. Molecular docking studies were against three protein cancer to investigate the binding ability of the new compounds. The anticancer activity was studied with different cell lines and the IC50 of the Cu(II) complex for (HOP-62) showed a practical result. The LD50 values of Zn(II) and Co(II) complexes showed that they are non-toxic at doses up to 370 mg/kg.