bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of chemometric-guided biomarkers associated with alcohol consumption in mice liver tissue using infrared spectroscopy
Authors: Qadawee Arwa Dahham , Algburi Ali Forat , Garip Ustaoğlu Şebnem , Dursun İlknur , Kaygusuz Hakan
Published: January 2024

Among the leading contributors to fetal alcohol spectrum disorder (FASD), perinatal alcohol usage is characterized by neurodevelopmental dysfunction and growth retardation. This study aims to determine spectroscopic biomarkers referring to the results of early postnatal exposure to alcohol in the liver of newborn mice. The samples were divided into three groups: Negative control group, gavage control group (positive control), and group with gavage + alcohol which is treated between postnatal days (PD) 3 and 20 with 3.0 g/kg body weight of ethanol in a liter of artificially enhanced milk (.02 ml/g). After the decapitation of mice, liver tissues were removed and infrared data were obtained using Fourier Transform Infrared (FTIR) Spectroscopy. Spectral data was analyzed and evaluated using normalization, principal component analysis (PCA), and hierarchical cluster analysis (HCA). Possible chemometric biomarkers detected in this study are related to the lipids and found as follows: 2960 cm−1, 2873 cm−1, 1454 cm−1, and 1398 cm−1. According to the results of this study, it can be proposed that FTIR is an effective tool for the detection of chemometric-guided biomarkers related to the consequences of early postnatal alcohol consumption on female rats.
Projection-Based Embedding with Projected Atomic Orbitals
Authors: Szirmai Ádám B. , Hégely Bence , Tajti Attila , Kállay Mihály , Szalay Péter G.
Published: January 2024

The projected atomic orbitals (PAO) technique is presented for the construction of virtual orbital spaces in projection-based embedding applications. The proposed straightforward procedure produces a set of virtual orbitals, which is used in the final, high-level calculation of the embedded active subsystem. The PAO scheme is demonstrated on intermolecular potentials of bimolecular complexes, in ground and excited states, including Rydberg excitations. The results show the outstanding performance of the PbE embedding method with PAO virtual orbitals compared to those produced using common orbital localization techniques. The good agreement of the resulting PbE potential curves with those from high-level ab initio dimer calculations, also in diffuse basis sets, confirms that the PAO technique can be suggested for future applications of this type. The use of D3 dispersion corrections in such calculations is also proposed, supported by a superior accuracy over dispersion terms sourced from the effective fragment potential (EFP2) model.
Application of cis-MoO2(OV)2 in Protection of Radiation-Induced DNA Damage
Authors: Deb Priyangana
Published: January 2024

This study investigates the synthesis of a molybdenum complex with a notable capacity to safeguard DNA against radiation-induced damage. Comprehensive analyses reveal that not only the complex [cis-MoO2(OV)2] exhibits a significant radioprotective efficacy which can shield 89% of damaged CT DNA but also the ligand (OV) show a good amount of protection. At a concentration of 2 mM and a radiation dosage of 20 Gy, cis-MoO2(OV)2 demonstrates the ability to protect approximately 85% of radiation-induced damage to plasmid (pUC19) DNA also from gamma rays. These findings underscore the considerable potential of cis-MoO2(OV)2 as an effective radioprotector for normal tissues in radiotherapy. Notably, the complex's capacity to mitigate DNA damage caused by radiation holds significant promise, presenting implications for the advancement of novel therapeutic strategies across various medical conditions.
Predicting Spin States of Iron Porphyrins with DFT Methods Including Crystal Packing Effects and Thermodynamic Corrections
Authors: Radon Mariusz
Published: January 2024

The accuracy of DFT methods for spin-state energetics is assessed by probing their ability to predict the experimental ground states of selected iron porphyrins in solid state. To this end, six Fe(III) or Fe(II) porphyrin complexes with experimentally assigned spin states and known crystal structures are taken under scrutiny. In each case, periodic and molecular density functional theory (DFT) calculations are employed to quantify how the crystal packing and porphyrin substituents affect their spin-state energetics. The crystal packing effect is resolved into the direct and structural components. The crystal packing and substituent effects are shown to greatly vary from case to case. It is proposed that by knowing the crystal packing and substituent effects, and the Gibbs free energy thermodynamic correction from calculations on a given experimentally characterized system, one can use the knowledge of the experimental ground state in order to derive a quantitative constrain on the electronic energy difference for the corresponding simplified (porphin) model. The constraints derived in such a way are used to assess the accuracy of dispersion-corrected DFT methods for spin-state energetics of [FeP(2-MeIm)2]+, [FeP(2-MeIm)]+, [FeP(THF)2] and FeP models (where P is porphin, 2-MeIm is 2-methylimidazole, THF is tetrahydrofuran). Relatively good accuracy is obtained in the case of double-hybrid functionals (B2PLYP-D3, DSD-PBEB95-D3). By contrast, hybrid functionals with reduced admixtures of exact exchange (B3LYP*-D3, TPSSh-D3), although often recommended in the literature for transition metal systems, are shown here to considerably overstabilize the intermediate spin state, especially in Fe(III) porphyrins. The new approach presented here, which can be generalized to other transition metal complexes, is not only useful in method benchmarking, but also sheds light on the interpretations of experimental data for e.g., mono- and bis-imidazole ligated Fe(III) porphyrins and square-planar Fe(II) porphyrin, which are important models to understand the electronic properties of heme prosthetic groups in metalloproteins.
télétravail transfrontalier face aux défis de coordination des règles de conflit et des normes minimales d'emploi
Authors: Chikoc Barreda Naivi
Published: January 2024

Le travail à distance effectué au moyen des technologies de l’information et des communications met en question le fondement traditionnel des règles cherchant à identifier la loi applicable aux contrats internationaux de travail. Dans un environnement virtuel, l’exécution de la prestation de travail défie les frontières nationales, employeurs et employés opérant désormais dans un marché numériqueet global. La dématérialisation de l’activité favorise la mobilité transfrontalière des télétravailleurs, intensifiant ainsi les difficultés dans la recherche d’un régime juridique qui s’applique de façon cohérente au rapport detravail potentiellement soumis à une diversité de lois. Le présent texte propose une réflexion comparative sur la réponse fournie par le droit international privé et par les règles qui délimitent unilatéralement le domaine territorial de la Loi sur les normes du travail au Québec. Sous un regard critique, nous soulignons les contradictions résultant d’une application non coordonnée de ces régimes. Pour remédier aux incohérences, nous défendons la voie de leur articulation, en justifiant sa pertinence d’un point de vue téléologique et en présentant les moyens techniques de sa réalisation.
Dispersive Elastic Moduli and Frequency-Dependent Attenuation due to Wave-Induced Fluid Flow in Metapelite
Authors: Fliedner Celine , French Melodie
Published: January 2024

Seismic waves are used to interpret geologic structure, composition, and environmental conditions in the Earth. However, rocks are not perfectly elastic and their viscoelasticity can dissipate energy during wave propagation. Wave-induced fluid flow mechanisms can cause viscoelasticity resulting in frequency-dependent attenuation, velocities, and elastic moduli (dispersion) in saturated rocks. Dispersion and attenuation are hypothesized to be important in subduction zones, where regions of high fluid content are interpreted below the seismogenic zone. However, this has not been well-tested because of a lack of measurements on relevant lithologies and under saturated conditions. We measured the Young's and shear moduli and the attenuation of a greenschist facies metapelite with the forced oscillation technique at frequencies between 2 x 10-5 and 30 Hz. The moduli and attenuation are frequency-dependent under saturated conditions and depend on the effective pressure. At relatively low effective pressure, the Young's and shear moduli increase by over 50 % between 2 x 10-5 and 30 Hz. We use Standard Linear Solid viscoelastic models to investigate the relationship between the attenuation and dispersion in the Orocopia schist. The models are consistent with the experimental data and demonstrate that viscoelasticity can cause significant dispersion and attenuation in subduction zones.
Features of professional burnout among employees of educational organizations
Authors:   Irkutsk State University, Popova V.V. , Reshetnikova E.V. ,   Irkutsk State University, Samusikova E. A. ,   Irkutsk State University
Published: January 2024

The results of a study on the topic of professional burnout among teachers are presented. The authors analyze the specifics of professional burnout and also present the results of a study of employees of educational institutions and teachers working in special correctional institutions. It were given recommendations for the prevention of professional burnout among teachers for managers and specialists of educational institutions based on the analysis of a theoretical review of the literature and an empirical study. It is intended for university teachers and students, workers educational institutions, social sector specialists and for everyone those who are interested in the problem of professional burnout.
KARAKTERISTIK MARSHALL ASPHALT CONCRETE-BINDER COURSE DENGAN BAHAN PENGISI BATU KARANG TAENO
Authors: Siahaya Vera Th. C. , Metekohy Selly , Wabula Rosmini , Matitaputty Josephus R.
Published: January 2024

Abstract

 

The coral rocks on Mount Taeno, Waringin Cap Village, Ambon, have the potential to be used as a filler for asphalt mixtures. In this research, an analysis of the use of filler material, which comes from Mount Taeno coral rocks, was carried out on the Marshall characteristics of asphalt mixtures, with a type of Asphalt Concrete-Binder Course. Asphalt mixtures are made with different asphalt contents, namely 5.0%; 5.5%; and 6.0%, and the filler content given to each mixture is 0%, 1%, 2%, and 3%. Evaluation was carried out on these mixtures using the 2018 General Specifications of the Bina Marga. This research shows that the asphalt content of the mixture influences the value of the optimum filler content added to the mixture. The higher the mixed asphalt content, the higher the optimum filler content that can be added. The optimum filler content for mixtures with asphalt content of 5.0%, 5.5%, and 6.0% is 1.30%, 1.58%, and 1.79%, respectively. The asphalt content in the Asphalt Concrete-Binder Course observed in this study was limited to only 5.0%, 5.5%, and 6.0%. Therefore, further studies are recommended to be carried out, on mixtures with asphalt content higher than 6.0%, with variations in filler content greater than 3.0%. Keywords: asphalt mixture; Marshall characteristics; filler; optimum filler content

 

 

Abstrak

 

Batu karang di Gunung Taeno, Dusun Waringin Cap, Ambon, berpotensi untuk dijadikan sebagai bahan pengisi campuran beraspal. Pada penelitian dilakukan analisis penggunaan bahan pengisi, yang berasal dari batu karang Gunung Taeno, terhadap karakteristik Marshall campuran beraspal, jenis Asphalt Concrete-Binder Course. Campuran beraspal dibuat dengan kadar aspal yang berbeda-beda, yaitu 5,0%; 5,5%; dan 6,0%, dan kadar bahan pengisi yang diberikan kepada masing-masin campuran tersebut adalah 0%, 1%, 2%, dan 3%. Evaluasi dilakukan terhadap campuran-campuran tersebut dengan menggunakan Spesifikasi Umum Bina Marga 2018. Penelitian ini menunjukkan bahwa kadar aspal campuran berpengaruh terhadap nilai kadar bahan pengisi optimum yang ditambahkan pada campuran. Semakin tinggi nilai kadar aspal campuran, semakin tinggi pula kadar bahan pengisi optimum yang dapat ditambahkan. Kadar bahan pengisi optimum untuk campuran dengan kadar aspal 5,0%, 5,5%, dan 6,0%, berturut-turut adalah 1,30%, 1,58%, dan 1,79%. Kadar aspal pada Asphalt Concrete-Binder Course yang diamati pada studi ini terbatas hanya 5,0%, 5,5%, dan 6,0%. Karena itu, kajian lebih lanjut disarankan untuk dilakukan, pada campuran dengan kadar aspal yang lebih tinggi daripada 6,0%, dengan variasi kadar bahan pengisi yang lebih besar daripada 3,0%.

 

Kata-kata kunci: campuran beraspal; karakteristik Marshall; bahan pengisi; kadar bahan pengisi optimum
Social imaginaries of tourists in the ruins of Armero – Tolima
Authors: Dominiccini Reyes Laura del Pilar , Mora Jorge Alexander , Bohorquez Patiño Luis Mauricio
Published: January 2024

Armero is a municipality located in the department of Tolima, Colombia, framed in the tragedy of the disaster that occurred on November 13, 1985. Hence, the development of this research focuses on inquiring why the tourist chooses to visit the municipality and his thoughts about dark tourism. The objective is to analyze the social imaginaries and the perception of the tourist, in a place mourned by events that triggered more than twenty-five thousand deaths. For this purpose, surveys were applied to 49 people who have visited the ruins of Armero, showing the interest of the participants in the places where dark tourism is practiced. As well as the feelings that the place produces, generating empathy with the locals. Finally, it was concluded that dark tourism in Armero can be a window to enter the practice with elements of sustainability and ethics to promote the memory of the territory.
A foodborne outbreak of Group A streptococcus: an under-recognised method of spread
Authors: Gatman Kate , Thompson Bryn , Harrower Jay , Rajanaidu Subha
Published: January 2024

Foodborne transmission of Group A Streptococcus (GAS) is a rare cause of pharyngitis outbreaks. This report details a GAS outbreak in New Zealand that was associated with a foodborne route of transmission. This outbreak was relevant in the New Zealand context given the high incidence of rheumatic fever (RF).
New migrants’ access to primary healthcare services in Aotearoa New Zealand
Authors: Pledger Megan , Buckley Sue , Cumming Jacqueline
Published: January 2024

aim: To explore new migrants’ access to primary healthcare services in the first 10 years after arrival in Aotearoa New Zealand. methods: Data come from three New Zealand Health Surveys (2014/2015, 2015/2016 and 2016/2017), which each sampled around 13,500 people, aged 15+ years, who were usual residents of Aotearoa New Zealand. Respondents who said they were born overseas were asked the first year they had come to Aotearoa New Zealand. Those who had arrived in the 10 years before their survey was completed were considered new migrants. The survey data were pooled and around 3,700 respondents were estimated to fit this category. Log-linear models, with adjustments for age, sex, ethnicity and New Zealand Deprivation Index, were used to look at last year use of primary healthcare. results: Overall, new migrants used primary healthcare similarly to other New Zealanders. They were more likely to have comprehensive health insurance and paid more for GP visits upon arrival but acted similarly to other New Zealanders after 4 years. conclusion: Generally, new migrants—after adjusting for covariates—appear to be accessing primary healthcare services in a similar manner to other New Zealanders, on average, soon after arrival.
Türkiye'de Briyofitlerle İlgili Lisansüstü Tezlerin Farklı Değişkenler Açısından Değerlendirilmesi
Authors: Cemek Serdar , Keçeli Tamer
Published: January 2024

Bu çalışmanın temel amacı Türkiye’de briyofitler (karayosunları, ciğerotları ve boynuzotları) üzerine yapılmış olan yüksek lisans ve doktora tezlerinin farklı değişkenler açısından incelenmesi ve analiz edilmesidir. Çalışmanın veri kaynağını Yükseköğretim Kurulu ulusal tez merkezine yüklenmiş olan lisansüstü tezler oluşturmaktadır. Tezlerin incelenmesi sonucunda briyofitlerle ilgili en çok lisansüstü tezin 23 adet ile Ankara Üniversitesi’nde yapıldığı, lisansüstü çalışmalardan %77.5’inin yüksek lisans, %22.5’inin ise doktora tezi olduğu tespit edilmiştir. Lisansüstü eğitim yapanların cinsiyetlerine göre dağılımına bakıldığında %53’ünün kadın, %47’sinin de erkek olduğu belirlenmiştir. Briyofitler üzerine araştırmaların yapıldığı üniversitelerin en çok Ankara’da olduğu, tezlerin ortalama 128 sayfadan oluştuğu, %80’inin açık erişiminin olduğu belirlenmiştir. Tezlerde en çok kullanılan anahtar kelimelerin ise karayosunu, flora, Türkiye, Bryophyta, ciğerotu, briyofit, karayosunları, ağır metal ve biyomonitör olduğu tespit edilmiştir.
In Transferral Between Chinese and American Cultures: Famous Chinese Short Stories, Retold by Lin Yutang and its Chinese Translation
Authors:   , Liu Min
Published: January 2024

Lin Yutang 林語堂 (1895–1976) was a Chinese American writer who wrote primarily in English and acted as an interpreter of Chinese culture for the west. During Lin’s stay in the United States from 1936 to 1966, he produced some thirty works in English to disseminate Chinese philosophy and customs to a western audience. Although Lin was hailed as a cultural ambassador between China and the United States, his translation and rewriting works are sometimes criticised for complying in the colonial power dynamics between China and the west. In this thesis, I demonstrate that Lin’s English translations and rewritings are not only achievements made from personal capabilities in bilingual transfer, but also products of individual efforts in dealing with cultural obstacles imposed by the authorities. I select Famous Chinese Short Stories, Retold by Lin Yutang as a case, to illustrate that Lin’s reworking on traditional Chinese folktale stories constitutes a reinvention of Chinese cultural legacy. Famous Chinese Short Stories, Retold by Lin Yutang contains a selection of twenty tales from both literati and popular sources in ancient Chinese culture, edited and rewritten in English by Lin using western storytelling techniques. As I argue, Lin’s cross-cultural rewriting helps to uncover the humanistic potential of traditional Chinese resources to modernise ancient Chinese society. Especially, his endeavour in translation and cross-cultural communication demonstrates his compassionate perspective on women’s status in ancient China. In addition, this thesis explores the Chinese translation of Famous Chinese Short Stories, Retold by Lin Yutang completed by a Taiwanese translator Zhang Zhenyu 張振玉, and examines the Chinese translation as part of the continued reception of Famous Chinese Short Stories, Retold by Lin Yutang in the Chinese world. As such, the study of Famous Chinese Short Stories, Retold by Lin Yutang provides a valuable reference for the significance of and challenges in translators’ cross-cultural mediation.
Efectividad de biomarcadores plasmáticos en la predicción de comportamiento suicida en pacientes adultos diagnosticados con depresión mayor: una revisión de alcance
Authors: Ruiz Salcedo Lina Maria
Published: January 2024

Introducción: La predicción del suicidio constituye un reto para el personal sanitario debido a la subjetividad de algunos de los factores que intervienen en este fenómeno, así como la dificultad en la medición de moléculas biológicas implicadas al nivel del sistema nervioso central o del ADN. Por esta razón, recientemente ha surgido el interés por biomarcadores plasmáticos para la predicción del comportamiento suicida en pacientes con trastornos mentales, especialmente aquellos diagnosticados con depresión mayor. El objetivo de la presente revisión es determinar la efectividad de biomarcadores plasmáticos en la predicción de comportamiento suicida en pacientes con depresión mayor.
Metodología: Se realizó una revisión de alcance basada en el marco metodológico propuesto por Kasaju, et al. Se identificaron publicaciones relevantes en las bases de datos PubMed y Embase durante octubre de 2022.
Resultados: Se encontraron diferencias significativas en los niveles plasmáticos de NPY, IL-1β, IL-6, IL-12, monocitos, glucosa, tenascina C, cortisol, quinurenina y triptófano entre pacientes diagnosticados con depresión mayor con comportamiento suicida y controles sanos.
Conclusión: La medición de biomarcadores plasmáticos es una potencial herramienta para la predicción del comportamiento suicida debido a su efectividad en pacientes diagnosticados con depresión mayor.
Desarrollo de depresión grave asociado al consumo excesivo de alimentos ultraprocesados en pacientes con diagnóstico de depresión leve: una revisión de alcance
Authors: Pozo Narváez William Nicolás
Published: January 2024

Introducción: Los trastornos mentales son de tratamiento y control complejo, y pueden originarse por distintos factores, entre estos la dieta. Actualmente, dentro de la dieta de las personas se encuentra la comida ultraprocesada por su alto contenido energético, fácil acceso y estímulo placentero. El objetivo de la presente revisión es conocer la asociación entre la progresión de los síntomas de los pacientes diagnosticados con depresión leve hacia el diagnóstico de depresión moderada o grave debido al excesivo consumo de alimentos ultraprocesados.

Metodología: En el presente artículo se realizó una revisión de alcance basada en la propuesta metodológica de Kasaju, et al., haciendo el uso de bases de datos como PubMed y Embase para la identificación de literatura relevante.
Resultados: Se encontró un total de 4 artículos en los que se evidenció una relación directa y bidireccional de la ingesta de alimentos ultraprocesados y el desarrollo de síntomas depresivos, según Bayes, J., et al, existe un bajo consumo de falimentos sanos, pero un alto de procesados en personas diagnosticadas con depresión, a diferencia de controles sanos (p<0.015) (Bayes et al., 2020). Se logran detectar la aparición de síntomas como el estrés y la ansiedad, relacionados directamente a depresión mayor, seguidos de la depresión en el paciente, asociados al alta ingesta de alimentos ultraprocesados en comparación con controles sanos (p<0.0001) (Lane et al., 2022a).
Conclusión: La exposición a la ingesta de comida procesada en se asocia de manera directa a la evolución de trastornos mentales como depresión.
ISOLATION, IDENTIFICATION, MOLECULAR, AND HISTOPATHOLOGICAL INVESTIGATIONS OF TWO PATHOGENIC ENTEROCOCCUS SPECIES FROM TILAPIA IN EGYPTIAN FARMS
Authors: El-Nobi Gamal , Hassanin Mohamed , El-Hady Mohamed , Aboshabana Shimaa
Published: January 2024

Abstract: The rapid increase in global aquaculture have exposed many diseases in aquaculture field, enterococci are one of them. Tilapia is one of the most important and economic fish species in the world. Hundred and thirty diseased farmed Nile tilapia (Oreochromis niloticus) had been investigated for Enterococci collected from different farms in Abbassa, Sahl Elhusseineya, Altal alkabir, Bahr Albaqar, Al Manzalah, Ismailia, Port said during both spring and summer seasons. Two species were identified by automated identification and antibiotic sensitivity testing system (ID&AST) as E. faecalis and E. faecium. In vitro antibiotic susceptibility testing for these two species showed high susceptibility to ampicillin, penicillin, streptomycin, and gentamycin while, E. faecalis was sensitive  to vancomycin but E. faecium was resistant to it. These species were confirmed by 16s rRNA as Enterococcus. The two species showed sensitivity and resistance to many antibiotics. Ninety fish with an average weight 50-250 g were exposed to pathogenicity test. Fish were randomly divided into three groups, each contain thirty fish then divided into triplicate (10 fish per replicate). Fish of the first and second groups were intraperitoneally injected with 0.2 ml of bacterial suspension of the 2 species, with comparison of a control group they showed high pathogenicity level (77% for E. faecium and 73% for E. faecalis), also the histopathology revealed vascular congestion and infiltration with inflammatory cells in examined organs tissues (brain, liver, kidney, spleen and heart). In conclusion, E. faecalis and E. faecium were the most common subspecies of Enterococci showing high pathogenicity for Nile tilapia fish in Egypt.Keywords: Identification, Tilapia, Enterococci, Pathogenicity, Histopathology,
THE IMMUNOSTIMULANT EFFECT OF SACCHAROMYCES CEREVISIAE AND THE IMPACT OF FUSARIUM SOLANI INFECTION ON OREOCHROMIS NILOTICUS
Authors: Abd El Ghany Nadia , Alagmy Gehan , Mohamed Abd elgalil Noha , Rasheed Neveen
Published: January 2024

Abstract:This study summarizes the impact of Fusarium solani on Oreochromis niloticus with evaluation of the efficacy of Saccharomyces cerevisiae for decreasing its possible consequences on fish health was investigated. Fifty samples of fish ration were collected for isolation and identification of Fusarium solani. One hundred and sixty O. niloticus clinically healthy, average weight of 59.25±1.04 g was distributed into four groups. First group was a control negative group (C). Second group (F) was infected with Fusarium solani (1x 104 zoospores of Fusarium /ml). Third group (F+S) was fed a basal diet containing S. cervisaie 1 g/ kg ration daily and injected intraperitoneally with Fusarium solani. Fourth group (S) was fed a basal diet containing (S. cervisaie) daily. The experiment extended for five weeks then all groups were challenged with Pseudomonas aeroginosa (3x 107 CFU/ml). Bacteriological findings of the intestinal microbiota revealed significant increase (log10 CFU/ml) in total bacterial count (TBC) in the (F+S) group (4.51±0.012) and decreased gradually from the 1st week to the 5th week, while the (S) group showed slight increase in TBC than (C) group (P<0.05). The haemato-immunological parameters after 3 weeks in the (S) group showed significant increase in serum lysozyme activity and total leukocytic count. After five weeks there were a marked decrease in serum lysozyme activity and total leukocytic count in (F) group. After the challenge, there was a significant decrease in serum lysozyme activity and total leukocytic count in (F) group. Lymphocyte count and gamma globulin were significantly higher in (S) group than group (C) (P<0.05). The Pathological lesions in gills, liver, muscles in (F) group showed sever degenerative changes, necrosis and leucocytic infiltrations. The lesions in group (F+S) were milder. It could concluded that S. cerevisiae can be used in ration of O. niloticus under immune-depressive stressful condition (fungal infection) to increase their resistance.       Key words: Fusarium solani, Oreochromis niloticus, Saccharomyces cerevisiae, immunostimulants.
EVALUATION OF CLINICAL FEATURES, ULTRASONOGRAPHY AND CARDIAC BIOMARKERS IN TRAUMATIC AND NON-TRAUMATIC PERICARDITIS IN COWS
Authors: Gouda Heba , El-Naggar Abbas , Eissa Aziza , Gouda Shaimaa , El- Zahar Heba
Published: January 2024

Abstract: A total of 82 female cows with signs of congestive heart failure (CHF) were examined clinically and echocardiography at Veterinary Teaching Hospital of Zagazig University, Egypt. Sixty-seven were diagnosed as traumatic pericarditis (TP) and 15 as non-traumatic pericarditis (non-TP). inappetence, fever, abnormal heart sounds, brisket edema were the most common clinical finding of both diseases. Recurrent tympany was an additional clinical sign in cows with TP. Respiratory acidosis, anemia, leukocytosis, and hyperfibrinogenemia were the main altered hematological parameters in both diseases.  There are significant increases in all cardiac biomarkers concentration (Cardiac troponin I (CTnI), Cardiac troponin T (CTnT), Creatine kinase myocardial band (CK-MB), Lactic dehydrogenase enzyme (LDH), amino-terminal pro B-type natriuretic peptide (NT-proBNP) and endothelin) in TP and non-traumatic pericarditis (non-TP). Whilst CTnI, CTnT, and CK-MB are the cardiac biomarkers that significantly differentiate between TP and non-TP. In conclusion, clinical evaluation couldn't differentiate between TP and non-TP while ultrasonography and CTnI, CTnT and CK-MB were of high diagnostic value for differentiation between both diseases.Keywords: Cardiac biomarkers; biochemical alterations; cattle; hematology; traumatic pericarditis; non-traumatic pericarditis.
ALLEVIATION HARMFUL EFFECTS OF NON- ALCOHOLIC FATTY LIVER DISEASE WITH PHYLLANTHUS NIRURI EXTRACT IN ALBINO RATS
Authors: Hashem Mohamed , Hashish Emad , Atteya Marwa
Published: January 2024

Non-alcoholic fatty liver disease (NAFLD) is a serious global health problem that is strongly linked to insulin resistance, obesity, and oxidative stress. The goal of current work was to inspect the hepatoprotective and hypolipidemic effects of Phyllanthus niruri ethanolic extract (PNEE) on high fat hyperlipidemic diet –induced rat's fatty liver. A unique high-fat diet (HFD) composed of coconut oil (7.3%), beef tallow (7.3%), safflower oil (0.4%) and cholesterol (1.5%) were used in the present study for induction of NAFLD or hyperlipidemia in male Albino rats. Forty-eight clinically healthy male Albino rats were equally divided into four groups: the first served as control. The second group, rats administered ethanol extract of Phyllanthus niruri (PN) (250 mg/kg b.wt.); while the third group was rats fed diet supplemented high fat concentrated (HFCD); and the fourth was rats received combination of HFCD and PN (HFCD+PN). All treatments were given daily by oral route for 105days. All treatments were given daily by oral route for 105 days. Treatment of hyperlipidemic rats by PNEE resulted in reduction of serum liver enzymes, and improvement in proteins and lipids profile, as well as pancreatic function tests, in comparison with hyperlipidemic rats (gp.3) which showed severe biochemical alterations. This was confirmed by histopathological findings in the liver. It could be concluded that PNEE treatment has a strong hepatoprotective impact against fatty alterations in animal models, suggesting that PNEE could be used as a promising agent to prevent fatty liver disease.
ELECTROCARDIOGRAPHY, ECHOCARDIOGRAPHY, AND VERTEBRAL HEART SCORE IN HEALTHY LOCAL EGYPTIAN MALE DOGS: BIAS CORRECTED BOOTSTRAP REFERENCE INTERVALS
Authors: El-said Basma , Mahmoud Abdel Raouf , Mohamed Sobhy , Gouda Shaimaa
Published: January 2024

The Egyptian domestic (Baladi) dog has been increasingly adopted as a companion animal. Breed-specific cardiovascular reference intervals (RIs) are useful for clinicians in identifying abnormalities. The study aimed to estimate RIs for different heart indices in healthy male Baladi dogs. Twenty-six dogs were recruited to the study. Dogs were approved to be healthy. Afterwards, they were consciously examined by electrocardiography, echocardiography, and radiography. RIs were estimated using a bias-corrected bootstrapping approach. Electrocardiographic RIs were heart rate: 103 – 132 (beats/minute); P: 0.02 - 0.04, T: 0.03 - 0.04 waves durations (seconds); P: 0.13 - 0.23, R: 1.19 - 1.51, T: 0.20 - 0.27 waves amplitudes (mV); and PR: 0.11 - 0.17, QRS: 0.06 - 0.08, QT: 0.41 - 0.44 intervals (seconds). Echocardiographic parameters included end (diastolic: 29.95 - 46.49, systolic: 5.54 - 10.32) volumes (ml); ejection fraction: 76.23 - 83.41 (%); stroke volume 25.13 - 37.41 (ml); fractional shortening: 0.43 - 0.51 (%); interventricular septal thickness (diastole: 7.61 - 8.55; systole: 12.19 - 14.12 mm); left atrial to aortic root ratio  (0.98 - 1.06); left ventricular diameter (diastole: 27.86 - 33.34, systole:14.42 - 18.14 mm) and left ventricular posterior wall thickness (diastole: 9.71 - 13.5; systole: 14.73 - 18.33 mm). Right lateral radiographs heart axes (long: 5.31 - 5.67, short 3.67 - 4.03 vertebrae) and VHS: 9.04 - 9.64 (vertebrae). No significant associations were identified between the measurements and body weight, age, and heart rate. In conclusions our results emphasized the importance of establishing Baladi‐specific RIs.
DEVELOPMENT AND VALIDATION OF A RP- HPLC METHOD FOR DETERMINATION OF DIFLOXACIN RESIDUES IN DIFFERENT RABBIT TISSUES
Authors: Said Ahmed , Abd Elaleem Abd Elaleem , El-Nabtity Sameh , Abd Elhafeez Sherief
Published: January 2024

The occurrence of antibiotic residues in food of animal origin is attributed to incorrect drug administration or neglecting withdrawal times. These residues cause a lot of human health hazards, so it is important to control their presence. The aim of our study to evaluate a reversed phase- high performance liquid chromatography (RP- HPLC) method for determination of difloxacin residues in different rabbit tissues and serum post intramuscular injection of 5 mg/kg b.wt for 5 days and to investigate the withdrawal period of difloxacin in different rabbit tissues to be safe for human consumption. This method was validated to meet United States Pharmacopeia (USP) and the International Council for Harmonisation of Technical Requirements for Pharmaceuticals (ICH) guidelines. The separation was processed using buffer of triethylamine (pH:3.5) and acetonitrile (80 to 20, v.to v.) solvent on RP- C18 column at 35oC to obtain the ideal peak at 6.752 min. the separation was achieved at wavelength of 280 nm. Validation of analytical method has been done to keep up specifications such as specificity, linearity, precision, accuracy, sensitivity, repeatability, and robustness. The study showed high recovery, low detection limit, good precision. In addition, the rapid steps of the extraction process to be more economical and meet the requirements of green chemistry through minimum use of chemicals during sample extraction with little chemical waste production. Finally, residues of difloxacin were less than recommended maximum residue limits (MRL); 300, 600, and 800 ppb for muscle, kidneys, and liver; respectively on 1st day after treatment. Thus, Rabbit`s meat could be eaten safely on the 1st day after the end of treatment with 5 mg difloxacin/kg for 5 days.
LATEX AGGLUTATION TEST AND PCR ASSAYS FOR DIAGNOSIS OF TOXOPLASMA GONDII INFECTION IN RED MEAT PRODUCING ANIMALS IN ASWAN GOVERNORATE, SOUTHERN EGYPT
Authors: Sameeh Shimaa , E. Mahmoud Abeer , El-Salahy MM Monib Mohamed , E. M. Eldeek Hanan
Published: January 2024

Livestock meat is considered a potential source of human infection, therefore evaluation of the infection rate with Toxoplasma gondii (T. gondii) in these animals’ meat is needed for public health protection. Serum and meat samples were collected from slaughtered animals (n=106) (27 sheep, 42 cattle and 37 camels) from slaughterhouses in Aswan Governorate, located in southern Egypt. T. gondii infection status was detected by Latex agglutination test (LAT), microscopic examination and PCR.  Our results revealed that the overall prevalence of anti-T. gondii antibodies among serum samples was 48.1 % using LAT. Male sheep, cattle, and camels had a higher positive percentage (42.9 %, 69.4% and 33.3 %, respectively) than females (40 %, 50 % and 30.8 %, respectively). The infection rate of T. gondii was higher in adult sheep and camels, while it was lower in adult cattle (64.5 %) than in young (72.7 %). There was a high statistically significant difference (P ≤ 0.01) between the animal species using LAT. T. gondii trophozoites were detected microscopically in 47.1 % of meat samples, while T. gondii DNA was detected in 56.9 % by using conventional Polymerase Chain Reaction (cPCR) based B1 gene. There was no significant difference between the results of both microscopy and PCR methods. In conclusion, PCR outperforms LAT in the detection of T. gondii infection and can thus be applied to routine diagnosis of toxoplasmosis in red meat producing animals.
INVESTIGATING THE BIOSECURITY MEASURES’ APPLICATIONS IN POULTRY FARMS AND ITS RELATIONSHIP WITH THE OCCURENCE OF AVIAN INFLUENZA
Authors: Ahmed Heba A. , Mohamed Mohamed E.M. , Erfan Ahmed M. , Abdelkarim Lubna , Awadallah Maysa A.I.
Published: January 2024

Biosecurity application in poultry farms means measures or practices which limit or prevent the spread of many harmful pathogens onto or out of the farm. When teamed with disinfection and sanitation procedures, biosecurity practices can eradicate or reduce pathogens to noninfectious levels and this illustrate that prevention is better than cure. Such preventive measures as vaccination and serologic monitoring also help to ensure good flock health. Inadequate biosecurity can contribute to creation of wide epidemics of highly pathogenic diseases such as avian influenza virus.  For data collection, a predesigned questionnaire was created, and two visits were applied for investigating the application of biosecurity measures in the selected farms. Results summarize the response of the farm workers regarding the application of biosecurity measures in 244 farms under investigation.  Regarding the number of farms with footbath, it was 150 (61%) in the first visit, although only 133 (56%) were filled with disinfectant. While, in the second visit, footbath number was significantly increased to 190 (78%) (χ12 = 12.68, P > 0.01) and 169 (69%) were filled with disinfectant (χ12 = 7.39, P > 0.01). The increased cleanliness of the area between the farm gate and poultry house was significantly increased from 68% in the first visit to 85% after the second visit (χ12 = 19.1, P > 0.01). As a result of the improved biosecurity measures observed in the second visit, the occurrence of avian influenza infection has significantly reduced from 36% to 20% in the farms under investigation. This in turn highlights the important role of biosecurity measures in the prevention and control of avian influenza infection. There is a need for more consideration on biosecurity measures targeting dead birds' disposal, changing shoes before entrance to the farm, presence of clothes for visitors, disinfection of injection tools,, disinfection machines and veterinary supervision.
GENETIC DIVERSITY AMONG TWO COMMON POPULATIONS OF CANIS LUPUS FAMILIARIS IN EGYPT BY USING MITOCHONDRIAL DNA HVR1 SEQUENCE
Authors: Elmadawy Mostafa A , El-Kassas Seham , Abdo Safaa E , Nagai Atsushi , Bunai Yasuo
Published: January 2024

The current study aimed to investigate the variation of mtDNA hypervariable region 1 (HVR1) among Egyptian Baladi and German shepherd dogs in Egypt with respect to their phylogenetic origin. Blood samples were obtained from two dog breeds; Egyptian Baladi (n = 46) and German shepherd (n = 42) and used for genomic DNA extraction and PCR amplification of the mtDNA HVR1 using primers H15360 and L16106. The determined haplotypes were aligned to the sequences of the first published dog mitochondrial genome (Accession No. U96639). We identified 22 different haplotypes from 34 single nucleotide polymorphisms (SNPs) including 2 insertion-deletion polymorphisms among the Egyptian Baladi dogs and 12 haplotypes from 22 SNPs among the German shepherd dogs. Four haplogroups (A, B, C, and D) were identified in the two breeds, their distribution includes 78% of Egyptian Baladi dogs and 76% of German shepherd dogs, respectively were located in the haplogroup A. While 19 % of the German shepherd and 15% of Egyptian Baladi dogs were found in the haplogroup B. 5% of the detected haplotypes of the two breeds were belonged to haplogroup C. 2% of the detected haplotypes of Egyptian Baladi dogs were classified to a haplogroup D. High haplotype and nucleotide diversities were found in the two breeds indicating a lack of genetic differentiation and a recent population growth. The later was confirmed in the Egyptian dogs with the negative values of the neutrality tests and their clustering in the same clade within the phylogenetic tree.
DIGESTIVE ENZYMES, IMMUNITY AND OXIDATIVE STATUS OF NILE TILAPIA (Oreochromis niloticus) REARED IN INTENSIVE CONDITIONS
Authors: Dawood Mahmoud A , Shukry Mustafa , Zayed Mohamed M , Omar Amira A , Zaineldin Amr I , El Basuini Mohammed F
Published: January 2024

High stocking density is significantly disturbing the growth and productivity of aquatic animals. Digestive enzymes, immunity and oxidative status of Nile tilapia were investigated in case of culturing in several densities. Fish (14.3±0.03 g) were stocked in 12 aquaria (70 L) at four densities of 10 (SD10), 20 (SD20), 30 (SD30) and 40 (SD40) fish per aquarium for 30 days. Fish growth, feed efficiency ratio, digestive enzyme activity and dissolved water oxygen significantly (P<0.05) decreased, while the total ammonia increased with increasing stocking density. Immunoglobulin and NBT levels decreased significantly (P<0.05) in SD40 set compared to SD20 set without no differences with the other two groups. Lysozyme activity reported the highest significant (P<0.05) values in SD10 and SD20 groups over the high stocking density group (SD40) without no difference with SD30 group. Bactericidal, phagocytic activities and phagocytic index reported significantly (P<0.05) lower values in fish reared in SD30 and SD40 groups than fish reared in SD10 and SD20 groups. Peroxidase activity also showed significantly (P<0.05) low values in SD40 and SD30 groups with the weakest activity in SD40 group. Total serum protein lowered relatively in SD30 and SD40 groups without no differences with the other groups. Furthermore, fish reared at high stocking densities resulted in significantly (P<0.05) decreased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities as well as increased malonaldehyde (MDA) activity in blood of tilapia suggesting suppressed antioxidant response. In conclusion, intensive conditions depressed the growth, digestive enzyme activity, immunity and oxidative status of Nile tilapia.Key words: digestive enzyme activity; growth; immunity; nile tilapia; oxidative status; stocking density
UTILIZATION OF SUGAR BEET BY PRODUCTS IN FEEDING GROW-ING RAHMANI LAMBS
Authors: Ali Mohamed , Eweedah Nabil , Gaafar Hamed , Abd El-Salam Metwally
Published: January 2024

Twenty female Rahmani lambs with an average initial body weight 31.01±0.68 kg were randomly divided into four similar groups (5 in each group). To receive one of the tested rations, Ration1 containing 50% concentrate feed mixture 1 plus 50% berseem hay as a control ration. In rations 2 and 4, 50% of berseem hay was replaced by dry sugar beet tops. While, in rations 3 and 4, 50% energy ingredients (yellow corn grains, wheat bran and rice bran) were replaced by dry sugar beet pulp in concentrate feed mixture 2. Ration 3 had significantly (P<0.05) the highest digestibility coefficient of all nutrients followed by rations 4 and 2, while, while ration 1 had the lowest values. Digestible crude protein had nearly the same trend, but total digestible nutrients and digestible energy were significantly higher in ration 3 compared with the other rations. A logarithmic scale of acidity or basicity of rumen liquor were nearly similar for all the different groups. Dry sugar beet pulp in rations 3 and 4 showed significantly the higher concentrations of total volatile fatty acids and ammonia nitrogen compared to the other rations. Dry sugar beet pulp increased dry matter, total digestible nutrients, digestible crude protein and digestible energy, but tended to decrease with dry sugar beet tops compared with control group. Growth rate and feed conversion were significantly higher (P<0.05) for lambs fed ration 3 compared with the other rations. Lambs fed ration 3 recorded significantly the highest net revenue and economic efficiency.Key words: growing lambs; dry sugar beet pulp; dry sugar beet tops; growth performance
EVALUASI PROGRAM SERTIFIKASI KEWIRAUSAHAAN UNTUK MENINGKATKAN MINAT DAN KOMPETENSI WIRAUSAHA DI UNIVERSITAS MUHAMMADIYAH PONOROGO
Authors: Arip Yusup , Putro Rizki Listyono , Ayuningtyas Eka Destriyanto Pristi
Published: January 2024

Tujuan penelitian ini adalah mengevaluasi keefektifan kegiatan kewirausahaan program Sertifikasi Kewirausahaan dalam meningkatkan bakat dan minat wirausaha mahasiswa. Subyek penelitian yaitu mahasiswa yang sudah menempuh Program Sertifikasi Kewirausahaan Universitas Muhammadiyah Ponorogo berjumlah 60. Penelitian ini menggunakan metode evaluasi CIPP dan temuan di lapangan menunjukkan 1)Context evaluation tujuan Program Sertifikasi Kewirausahaan yaitu salah satunya terkait maindset kewirausahaan. 2)Input evaluation berupa kegiatan action dilapanagan. 3)Process evaluation berupa mentoring bersama. 4)Product evaluation bahwa secara teori mereka mengerti tentang karakter yang dibutuhkan oleh seorang wirausaha. Saran untuk memperbaiki program ini yaitu perlu melakukan perbaikan pola, kurikulum, silabi dalam kegiatan pembejaran secara bertahap, karena di kegiatan ini mahasiswa dilibatkan dalam konteks kehidupan nyata (melihat peluang usaha).Kata Kunci: sertifikasi kewirausahaan, entrepreneur, wirausaha.
PERDAGANGAN MERCHANDISE SEBAGAI DETERMINAN INVESTASI ASING LANGSUNG DI COTE D’IVOIRE
Authors: Hertikasari Ainun , Artha Bhenu , Manggala Kasma , Gea Lidiani Erna
Published: January 2024

Perkiraan bahwa akan ada hubungan yang kuat antara investasi asing langsung dan perdagangan barang, kurang jelas apakah dampak perdagangan terhadap investasi asing langsung seharusnya berbeda untuk negara-negara yang sedang berkembang atau apakah arus masuk dan keluar investasi asing langsung bereaksi secara berbeda dengan berbagai jenis arus perdagangan. Berkurangnya hambatan yang menghalangi arus barang dan modal lintas batas telah mendorong lonjakan perdagangan internasional dan investasi asing langsung. Penelitian ini bertujuan untuk mengetahui pengaruh perdagangan merchandise terhadap investasi asing langsung di Cote d’Ivoire, dengan menggunakan metode kuantitatif. Data yang digunakan diambil dari Bank Dunia. Data dalam penelitian ini dianalisis menggunakan regresi. Hasil analisis memperlihatkan bahwa investasi asing langsung tidak dipengaruhi oleh perdagangan merchandise di Cote d’Ivoire. Saran untuk penelitian selanjutnya juga disampaikan disini. Kata Kunci:  investasi, perdagangan, negara berkembang
Uçucu Kül ve Seramik Sektörü Atıklarının Tekli, İkili Ve Üçlü Kullanımı İle Üretilen Geopolimer Harçların Dayanım Özellikleri
Authors: ESER Ayşe , BAYER OZTÜRK Zahide , ATABEY İsmail İsa , ÇELİKTEN Serhat
Published: January 2024

Türkiye'de seramik sektörü, ekonomik anlamda önemli bir paya sahip olup, ülkemiz seramik kaplama malzemeleri ve vitrifiye üretiminde lider konumdadır. Çanak-çömlek ürünleri seramik üretiminin bir başka kolunu oluşturmaktadır. Ancak, ilgili sektörde ıskartaya ayrılan hatalı ürünlerden kaynaklanan atıkların oluşması çevre kirliliği, depolama, tozlaşma sorunlarını beraberinde getirmekte ve atık üretim miktarı dikkate alındığında sektör atıklarının farklı alanlarda değerlendirilmesini zorunlu kılmaktadır. Bu çalışmada, seramik-karo fabrikası filtre-pres kek, vitrifiye ve çömlek atıklarının, farklı aktivatörlerle uçucu kül ile ve kül olmadan üretilen geopolimer harçların mekanik özellikleri incelenmiştir. Sıvı/bağlayıcı oranı 0.40 olan, NaOH ve Na2SiO3 içeren harç karışımları 24 saat boyunca 90ºC sıcaklıkta ısıl küre tabi tutulmuştur. Numunelere 7, 28 günlük eğilme ve basınç dayanımı deneyleri uygulanmıştır. Na2SiO3 ile üretilen harçların eğilme dayanımlarının NaOH ile üretilenlere göre daha yüksek olduğu, basınç dayanımlarında ise seramik atıklarının ikili ve uçucu küllü üçlü karışımlarının 30 MPa’dan yüksek değerlere ulaştığı belirlenmiştir. Çalışma seramik sektörü atıklarının geopolimer üretiminde başarıyla değerlendirilebileceğini ortaya koymaktadır.