bluuebunny/crossref_metadata_2025 huggingface dataset:
Pathogenesis, Early Diagnosis, and Therapeutic Management of Alcoholic Liver Disease
Authors: Kong Ling-Zu , Chandimali Nisansala , Han Ying-Hao , Lee Dong-Ho , Kim Ji-Su , Kim Sun-Uk , Kim Tae-Don , Jeong Dong Kee , Sun Hu-Nan , Lee Dong Sun , Kwon Taeho
Published: June 2019

Alcoholic liver disease (ALD) refers to the damages to the liver and its functions due to alcohol overconsumption. It consists of fatty liver/steatosis, alcoholic hepatitis, steatohepatitis, chronic hepatitis with liver fibrosis or cirrhosis, and hepatocellular carcinoma. However, the mechanisms behind the pathogenesis of alcoholic liver disease are extremely complicated due to the involvement of immune cells, adipose tissues, and genetic diversity. Clinically, the diagnosis of ALD is not yet well developed. Therefore, the number of patients in advanced stages has increased due to the failure of proper early detection and treatment. At present, abstinence and nutritional therapy remain the conventional therapeutic interventions for ALD. Moreover, the therapies which target the TNF receptor superfamily, hormones, antioxidant signals, and MicroRNAs are used as treatments for ALD. In particular, mesenchymal stem cells (MSCs) are gaining attention as a potential therapeutic target of ALD. Therefore, in this review, we have summarized the current understandings of the pathogenesis and diagnosis of ALD. Moreover, we also discuss the various existing treatment strategies while focusing on promising therapeutic approaches for ALD.
Acute Myeloid Leukemia Mutations: Therapeutic Implications
Authors: Papayannidis Cristina , Sartor Chiara , Marconi Giovanni , Fontana Maria Chiara , Nanni Jacopo , Cristiano Gianluca , Parisi Sarah , Paolini Stefania , Curti Antonio
Published: June 2019

Acute Myeloid Leukemia (AML) is an extremely heterogeneous group of hematological neoplasms, for which allogeneic stem cell transplantation (HSCT) still represents the only potentially curative option in the majority of cases. However, elderly age and clinically severe comorbidities may often exclude a wide amount of patients from this therapeutic approach, underlying the urgent need for alternative strategies. Thanks to the introduction of advanced high-throughput techniques, light is being shed on the pathogenesis of AML, identifying molecular recurrent mutations as responsible for the onset, as well as progression, of disease. As a consequence, and in parallel, many new compounds, including targeted therapies (FMS-like tyrosine kinase 3 (FLT3) and Isocitrate dehydrogenase 1-2 (IDH1-2) inhibitors), have found a wide room of application in this setting, and are now available in daily practice, or in late phases of clinical development. Moreover, several further innovative molecules are currently under investigation, and promising results for many of them have already been reported. In this review, we will present an update on the most relevant molecular alterations of AML, focusing on the most frequent genomic mutations of the disease, for which compounds have been approved or are still currently under investigation.
Analysis of Sildenafil in Liquor and Health Wine Using Surface Enhanced Raman Spectroscopy
Authors: Xiao Shupei , He Yong
Published: June 2019

The illegal adulteration of sildenafil in herbal food supplements and alcoholic drinks immensely threatens human health due to its harmful side-effects. Therefore, it is important to accurately detect and identify the presence of sildenafil in alcoholic drinks. In this study, Opto Trace Raman 202 (OTR 202) was used as surface enhanced Raman spectroscopy (SERS) active colloids to detect sildenafil. The results demonstrated that the Raman enhancement factor (EF) of OTR 202 colloids reached 1.84 × 107 and the limits of detection (LODs) of sildenafil in health wine and liquor were found to be as low as 0.1 mg/L. Moreover, the SERS peaks of 645, 814, 1235, 1401, 1530 and 1584 cm−1 could be qualitatively determined as sildenafil characteristic peaks and the relationship between Raman peak intensity and sildenafil concentration in health wine and liquor were different. There was a good linear correlation between Raman peak intensity, and sildenafil concentration in health wine ranged 0.1–1 mg/L (0.9687< R2 < 0.9891) and 1–10 mg/L (0.9701 < R2 < 0.9840), and in liquor ranged 0.1–1 mg/L (0.9662 < R2 < 0.9944) and 1–20 mg/L (0.9625 < R2 < 0.9922). The relative standard deviations (RSD) were less than 5.90% (sildenafil in health wine) and 9.16% (sildenafil in liquor). The recovery ranged 88.92–104.42% (sildenafil in health wine) and 90.09–104.55% (sildenafil in liquor). In general, the sildenafil in health wine and liquor could be rapidly and quantitatively determined using SERS technique, which offered a simple and accurate alternative for the determination of sildenafil in alcoholic drinks.
Effects of Dapagliflozin on Volume Status When Added to Renin–Angiotensin System Inhibitors
Authors: Eickhoff Mie K. , Dekkers Claire C. J. , Kramers Bart J. , Laverman Gozewijn Dirk , Frimodt-Møller Marie , Jørgensen Niklas Rye , Faber Jens , Danser A. H. Jan , Gansevoort Ron T. , Rossing Peter , Persson Frederik , Heerspink Hiddo J. L.
Published: June 2019

Sodium glucose co-transporter 2 (SGLT2) inhibitors reduce the risk of heart and kidney failure in patients with type 2 diabetes, possibly due to diuretic effects. Previous non-placebo-controlled studies with SGLT2 inhibitors observed changes in volume markers in healthy individuals and in patients with type 2 diabetes with preserved kidney function. It is unclear whether patients with type 2 diabetes and signs of kidney damage show similar changes. Therefore, a post hoc analysis was performed on two randomized controlled trials (n = 69), assessing effects of dapagliflozin 10 mg/day when added to renin–angiotensin system inhibition in patients with type 2 diabetes and urinary albumin-to-creatinine ratio ≥30 mg/g. Blood and 24-h urine was collected at the start and the end of treatment periods lasting six and 12 weeks. Effects of dapagliflozin compared to placebo on various markers of volume status were determined. Fractional lithium excretion, a marker of proximal tubular sodium reabsorption, was assessed in 33 patients. Dapagliflozin increased urinary glucose excretion by 217.2 mmol/24 h (95% confidence interval (CI): from 155.7 to 278.7, p < 0.01) and urinary osmolality by 60.4 mOsmol/kg (from 30.0 to 90.9, p < 0.01), compared to placebo. Fractional lithium excretion increased by 19.6% (from 6.7 to 34.2; p < 0.01), suggesting inhibition of sodium reabsorption in the proximal tubule. Renin and copeptin increased by 46.9% (from 21.6 to 77.4, p < 0.01) and 33.0% (from 23.9 to 42.7, p < 0.01), respectively. Free water clearance (FWC) decreased by −885.3 mL/24 h (from −1156.2 to −614.3, p < 0.01). These changes in markers of volume status suggest that dapagliflozin exerts both osmotic and natriuretic diuretic effects in patients with type 2 diabetes and kidney damage, as reflected by increased urinary osmolality and fractional lithium excretion. As a result, compensating mechanisms are activated to retain sodium and water.
Systematic Review of the Long-Term Effects of Transgender Hormone Therapy on Bone Markers and Bone Mineral Density and Their Potential Effects in Implant Therapy
Authors: Delgado-Ruiz Rafael , Swanson Patricia , Romanos Georgios
Published: June 2019

This study seeks to evaluate the long-term effects of pharmacologic therapy on the bone markers and bone mineral density of transgender patients and to provide a basis for understanding its potential implications on therapies involving implant procedures. Following the referred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and well-defined PICOT (Problem/Patient/Population, Intervention, Comparison, Outcome, Time) questionnaires, a literature search was completed for articles in English language, with more than a 3 year follow-up reporting the long-term effects of the cross-sex pharmacotherapy on the bones of adult transgender patients. Transgender demographics, time under treatment, and treatment received were recorded. In addition, bone marker levels (calcium, phosphate, alkaline phosphatase, and osteocalcin), bone mineral density (BMD), and bone turnover markers (Serum Procollagen type I N-Terminal pro-peptide (PINP), and Serum Collagen type I crosslinked C-telopeptide (CTX)) before and after the treatment were also recorded. The considerable variability between studies did not allow a meta-analysis. All the studies were completed in European countries. Transwomen (921 men to female) were more frequent than transmen (719 female to male). Transwomen’s treatments were based in antiandrogens, estrogens, new drugs, and sex reassignment surgery, meanwhile transmen’s surgeries were based in the administration of several forms of testosterone and sex reassignment. Calcium, phosphate, alkaline phosphatase, and osteocalcin levels remained stable. PINP increased in transwomen and transmen meanwhile, CTX showed contradictory values in transwomen and transmen. Finally, reduced BMD was observed in transwomen patients receiving long-term cross-sex pharmacotherapy. Considering the limitations of this systematic review, it was concluded that long-term cross-sex pharmacotherapy for transwomen and transmen transgender patients does not alter the calcium, phosphate, alkaline phosphatase, and osteocalcin levels, and will slightly increase the bone formation in both transwomen and transmen patients. Furthermore, long-term pharmacotherapy reduces the BMD in transwomen patients.
A Comparison Study of Marginal and Internal Fit Assessment Methods for Fixed Dental Prostheses
Authors: Son Keunbada , Lee Sangbong , Kang Seok Hyon , Park Jaeseok , Lee Kyu-Bok , Jeon Mansik , Yun Byoung-Ju
Published: June 2019

Numerous studies have previously evaluated the marginal and internal fit of fixed prostheses; however, few reports have performed an objective comparison of the various methods used for their assessment. The purpose of this study was to compare five marginal and internal fit assessment methods for fixed prostheses. A specially designed sample was used to measure the marginal and internal fit of the prosthesis according to the cross-sectional method (CSM), silicone replica technique (SRT), triple scan method (TSM), micro-computed tomography (MCT), and optical coherence tomography (OCT). The five methods showed significant differences in the four regions that were assessed (p < 0.001). The marginal, axial, angle, and occlusal regions showed low mean values: CSM (23.2 µm), TSM (56.3 µm), MCT (84.3 µm), and MCT (102.6 µm), respectively. The marginal fit for each method was in the range of 23.2–83.4 µm and internal fit (axial, angle, and occlusal) ranged from 44.8–95.9 µm, 84.3–128.6 µm, and 102.6–140.5 µm, respectively. The marginal and internal fit showed significant differences depending on the method. Even if the assessment values of the marginal and internal fit are found to be in the allowable clinical range, the differences in the values according to the method should be considered.
Experimental and Numerical Investigations of Thin-Walled Stringer-Stiffened Panels Welded with RFSSW Technology under Uniaxial Compression
Authors: Kubit Andrzej , Trzepiecinski Tomasz , Święch Łukasz , Faes Koen , Slota Jan
Published: June 2019

Many aircraft structures are thin walled and stringer-stiffened, and therefore, prone to a loss of stability. This paper deals with accurate and validated stability analysis of the model of aircraft skin under compressive loading. Both experimental and numerical analyzes are conducted. Two different methods of joining panel elements are considered. In the first case, the panel is fabricated using rivets. In the second variant, the refill friction stir spot welding technique is used. Both types of panels are loaded in axial compression in a uniaxial tensile testing machine. The geometrically and physically nonlinear finite element analyzes of the panels were carried out in ABAQUS/Standard. The Digital Image Correlation measurement system ARAMIS has been utilized to monitor the buckling behavior and failure mode in the skin-stringer interface of the stiffened panels. The results of experiments and the digital image correlation system are presented and compared to the numerical simulations.
Effect of Heat Curing Method on the Mechanical Strength of Alkali-Activated Slag Mortar after High-Temperature Exposure
Authors: Tran Tai Thanh , Kang Hyuk , Kwon Hyug-Moon
Published: June 2019

The aim of this work was to study the mechanical strength and microstructure changes of alkali-activated slag mortar (AAS mortar) after being heat treated in the temperature range of 200–1000 °C. The AAS mortar was cured in the ambient condition (20 ± 5 °C, 60 ± 5% RH) (Relative humidity: RH) and high temperature condition (80 °C) for 27 days with three different heating regimes: curing in a dry oven, curing in sealed plastic bags, and in a steam environment. The activator for the AAS synthesis was a mixture of sodium silicate solution (water glass) and sodium hydroxide (NaOH) with a SiO2/Na2O weight ratio of 1, and a dosage of 4% Na2O by slag weight. Thermogravimetric analysis (TGA) and scanning electron microscopy (SEM) incorporated with energy-dispersive X-ray spectroscopy (EDX) were used to assess the mortar microstructure change. The results revealed that the curing method significantly affected the mechanical strength of AAS at temperatures lower than 800 °C. The heat treatment at late age of 28 days was more beneficial for compressive strength enhancement in specimens without using heat curing methods.
Determination of Displacement Fields at the Sub-Nanometric Scale
Authors: Sciammarella Cesar A. , Sciammarella Federico M. , Lamberti Luciano
Published: June 2019

Macroscopic behavior of materials depends on interactions of atoms and molecules at nanometer/sub-nanometer scale. Experimental mechanics (EM) can be used for assessing relationships between the macro world and the atomic realm. Theoretical models developed at nanometric and sub-nanometric scales may be verified using EM techniques with the final goal of deriving comprehensive but manageable models. Recently, the authors have carried out studies on EM determination of displacements and their derivatives at the macro and microscopic scales. Here, these techniques were applied to the analysis of high-resolution transmission electron microscopy patterns of a crystalline array containing dislocations. Utilizing atomic positions as carriers of information and comparing undeformed and deformed configurations of observed area, displacements and their derivatives, as well as stresses, have been obtained in the Eulerian description of deformed crystal. Two approaches are introduced. The first establishes an analogy between the basic crystalline structure and a 120° strain gage rosette. The other relies on the fact that, if displacement information along three directions is available, it is possible to reconstruct the displacement field; all necessary equations are provided in the paper. Remarkably, the validity of the Cauchy-Born conjecture is proven to be correct within the range of observed deformations.
Sea Anemone Toxins: A Structural Overview
Authors: Madio Bruno , King Glenn F. , Undheim Eivind A. B.
Published: June 2019

Sea anemones produce venoms of exceptional molecular diversity, with at least 17 different molecular scaffolds reported to date. These venom components have traditionally been classified according to pharmacological activity and amino acid sequence. However, this classification system suffers from vulnerabilities due to functional convergence and functional promiscuity. Furthermore, for most known sea anemone toxins, the exact receptors they target are either unknown, or at best incomplete. In this review, we first provide an overview of the sea anemone venom system and then focus on the venom components. We have organised the venom components by distinguishing firstly between proteins and non-proteinaceous compounds, secondly between enzymes and other proteins without enzymatic activity, then according to the structural scaffold, and finally according to molecular target.
Nutrient Deprivation-Associated Changes in Green Microalga Coelastrum sp. TISTR 9501RE Enhanced Potent Antioxidant Carotenoids
Authors: Rauytanapanit Monrawat , Janchot Kantima , Kusolkumbot Pokchut , Sirisattha Sophon , Waditee-Sirisattha Rungaroon , Praneenararat Thanit
Published: June 2019

The utilization of microalgae as a source of carotenoid productions has gained increasing popularity due to its advantages, such as a relatively fast turnaround time. In this study, a newly discovered Coelastrum sp. TISTR 9501RE was characterized and investigated for its taxonomical identity and carotenoid profile. To the best of our knowledge, this report was the first to fully investigate the carotenoid profiles in a microalga of the genus Coelastrum. Upon use of limited nutrients as a stress condition, the strain was able to produce astaxanthin, canthaxanthin, and lutein, as the major carotenoid components. Additionally, the carotenoid esters were found to be all astaxanthin derivatives, and β-carotene was not significantly present under this stress condition. Importantly, we also demonstrated that this practical stress condition could be combined with simple growing factors, such as ambient sunlight and temperature, to achieve even more focused carotenoid profiles, i.e., increased overall amounts of the aforementioned carotenoids with fewer minor components and chlorophylls. In addition, this green microalga was capable of tolerating a wide range of salinity. Therefore, this study paved the way for more investigations and developments on this fascinating strain, which will be reported in due course.
Marine-Derived Anticancer Agents: Clinical Benefits, Innovative Mechanisms, and New Targets
Authors: Pereira Renato B. , Evdokimov Nikolai M. , Lefranc Florence , Valentão Patrícia , Kornienko Alexander , Pereira David M. , Andrade Paula B. , Gomes Nelson G. M.
Published: June 2019

The role of the marine environment in the development of anticancer drugs has been widely reviewed, particularly in recent years. However, the innovation in terms of clinical benefits has not been duly emphasized, although there are important breakthroughs associated with the use of marine-derived anticancer agents that have altered the current paradigm in chemotherapy. In addition, the discovery and development of marine drugs has been extremely rewarding with significant scientific gains, such as the discovery of new anticancer mechanisms of action as well as novel molecular targets. Approximately 50 years since the approval of cytarabine, the marine-derived anticancer pharmaceutical pipeline includes four approved drugs and eighteen agents in clinical trials, six of which are in late development. Thus, the dynamic pharmaceutical pipeline consisting of approved and developmental marine-derived anticancer agents offers new hopes and new tools in the treatment of patients afflicted with previously intractable types of cancer.
Synthesis and Preliminary Biological Evaluation of Two Fluoroolefin Analogs of Largazole Inspired by the Structural Similarity of the Side Chain Unit in Psammaplin A
Authors: Zhang Bingbing , Shan Guangsheng , Zheng Yinying , Yu Xiaolin , Ruan Zhu-Wei , Li Yang , Lei Xinsheng
Published: June 2019

Largazole, isolated from a marine Cyanobacterium of the genus Symploca, is a potent and selective Class I HDAC (histone deacetylation enzymes) inhibitor. This natural 16-membered macrocyclic depsipeptide features an interesting side chain unit, namely 3-hydroxy-7-mercaptohept-4-enoic acid, which occurs in many other natural sulfur-containing HDAC inhibitors. Notably, one similar fragment, where the amide moiety replaces the trans alkene moiety, appears in Psammaplin A, another marine natural product with potent HDAC inhibitory activities. Inspired by such a structural similarity, we hypothesized the fluoroolefin moiety would mimic both the alkene moiety in Largazole and the amide moiety in Psammaplin A, and thus designed and synthesized two novel fluoro olefin analogs of Largazole. The preliminary biological assays showed that the fluoro analogs possessed comparable Class I HDAC inhibitory effects, indicating that this kind of modification on the side chain of Largazole was tolerable.
Baking Effect on Desorption of Diffusible Hydrogen and Hydrogen Embrittlement on Hot-Stamped Boron Martensitic Steel
Authors: Kim Hye-Jin , Park Hyeong-Kwon , Lee Chang-Wook , Yoo Byung-Gil , Jung Hyun-Yeong
Published: June 2019

Recently, hot stamping technology has been increasingly used in automotive structural parts with ultrahigh strength to meet the standards of both high fuel efficiency and crashworthiness. However, one issue of concern regarding these martensitic steels, which are fabricated using a hot stamping procedure, is that the steel is highly vulnerable to hydrogen delayed cracking caused by the diffusible hydrogen flow through the surface reaction of the coating in a furnace atmosphere. One way to make progress in understanding hydrogen delayed fractures is to elucidate an interaction for desorption with diffusible hydrogen behavior. The role of diffusible hydrogen on delayed fractures was studied for different baking times and temperatures in a range of automotive processes for hot-stamped martensitic steel with aluminum- and silicon-coated surfaces. It was clear that the release of diffusible hydrogen is effective at higher temperatures and longer times, making the steel less susceptible to hydrogen delayed fractures. Using thermal desorption spectroscopy, the phenomenon of the hydrogen delayed fracture was attributed to reversible hydrogen in microstructure sites with low trapping energy.
Microstructure and Mechanical Properties of a Fiber Welded Socket-Joint Made of Powder Metallurgy Molybdenum Alloy
Authors: Xie Miao-Xia , Li Yan-Xin , Shang Xiang-Tao , Wang Xue-Wu , Pei Jun-Yu
Published: June 2019

Fiber welding of socket-joints made of nanostructured high-performance molybdenum alloy (NS Mo) was carried out to get a better understanding of the role of welding heat input. It was found that low heat input (i.e., high welding speed) resulted in significantly refined grains in the fusion zone (FZ) of fiber laser welded NS Mo joints. When welding heat input decreased from 3600 J/cm (i.e., 1.2 kW, 20 cm/min) to 250 J/cm (i.e., 2.5 kW, 600 cm/min), the tensile strength of welded joints increased from about 250 MPa to about 570 MPa. It was confirmed by energy spectrum analysis that the higher the welding heat input, the higher the oxygen contents at the grain boundary (GB) within the FZ. In addition, the most important reason for poor strength of welded joints of Mo alloys was reported as being that MoO2 was segregated on the grain boundary. Therefore, it was concluded that welding under low heat input (i.e., high welding speed) was able to reduce the segregation degree of MoO2 at the grain boundary by refining grains and increasing the total area of GBs, thus improving the strength of welded joints and reducing the proportion of the intergranular fracture zone in tensile fractures.
Method Development for Selected Bisphenols Analysis in Sweetened Condensed Milk from a Can and Breast Milk Samples by HPLC–DAD and HPLC-QqQ-MS: Comparison of Sorbents (Z-SEP, Z-SEP Plus, PSA, C18, Chitin and EMR-Lipid) for Clean-Up of QuEChERS Extract
Authors: Tuzimski Tomasz , Szubartowski Szymon
Published: June 2019

Background: Identification and quantitative determination of analytes released from the packaging material is undoubtedly a difficult and tricky task, requiring the chemical analyst to develop an individual approach to obtain reliable analytical information. Unfortunately, it is still challenging for scientists to determine bisphenols at trace or even ultra-trace levels in samples characterized by a very complex, and often variable, matrix composition. Objective: Optimization and application of QuEChERS/d-SPE coupled with HPLC-DAD (and LC-QqQ-MS) method for the simultaneous determination of bisphenols (A, S, F, B, BADGE and derivatives) in milk samples from a can and breast milk samples have been performed. Methods: Concerning the analysis of unconjugated analytes, after the thawing and shaking the sample (5 mL breast milk or 10 mL milk samples from a can), it was transferred into a 50 mL polypropylene centrifuge tube. For the analysis of the total amount of analytes, prior to the extraction with acetonitrile, a deconjugation step was implemented in a tube by adding to sample, the an Isotopically Labelled Internal Standard (IS) solution (50 ng/mL) and 1 mL of the enzymatic solution with the β-Glucuronidase (3500 U/mL). The mix was homogenized and incubated for 16–18 h at 37 °C. Next, 10 mL of acetonitrile, and a QuEChERS salt packet (4 g anhydrous MgSO4, 1 g NaCl) were added. After shaking and centrifugation, the total acetonitrile layer was isolated in a polypropylene tube evaporate to dryness, and reconstitute in 1.2 mL acetonitrile. During d-SPE step the extract was transferred into a 15 mL polypropylene tube with Z-Sep and primary secondary amine (PSA). Next, shake the tube, store in fridge, and centrifuge for 15 min. The acetonitrile supernatant was obtained with a pipette and evaporated to dryness. Mixture MeOH: water (20:80, v/v) were added to the dry residue and the extract was reconstitute in 200 μL and analyzed by HPLC-DAD and HPLC–QqQ-MS equipment. Conclusion: Six different salts during d-SPE step were evaluated such as: zirconium dioxide-based sorbent (Z-Sep, Z-Sep Plus), primary secondary amine (PSA), octadecyl (C18), EMR-Lipid, Chitin and also their mixtures. Negligible matrix interference was observed for most of the analytes due to application of Z-Sep and PSA in dispersive-solid phase extraction clean-up step. Extraction of target analytes was performed using QuEChERS/d-SPE cleanup, and presents good performance for selected analytes with recoveries in the range of 15–103% and relative standard deviations (RSD) less than 10% in breast milk samples.
Wetting Behavior and Maximum Retention of Aqueous Surfactant Solutions on Tea Leaves
Authors: Zhu Feng , Cao Chong , Cao Lidong , Li Fengmin , Du Fengpei , Huang Qiliang
Published: June 2019

In this research, the maximum retention and wetting behavior of surfactant solutions (N-200, N-300, Tween-80, Morwet EFW, DTAB, SDS) on the surfaces of tea leaves was investigated based on surface free energy, surface tension, the contact angle, adhesion work, and adhesion force. The results showed that the contact angles of all surfactant solutions were kept constant with low adsorption at the tea leaf–liquid interfaces below 0.005%. With an increase in concentration, the contact angle of Tween-80 decreased sharply because the adsorption of molecules at the solid–liquid interfaces (ΓSL’) was several times greater than that at the liquid–air interfaces (ΓLV). Adhesion work decreased sharply and then reached a minimum at the critical micelle concentration (CMC), but then increased until reaching a constant. Moreover, a high adhesion force did not indicate better wettability, as it does with rose petals and peanut leaves. For tea leaf surfaces, an increase in the contact angle brought about an increase in the adhesion force. In addition, the maximum retention for Morwet EFW is at different concentrations compared to N-200, N-300, Tween-80, DTAB, and SDS, where the maximum retention of Morwet EFW on tea leaves was 6.05 mg/cm2 at 0.005%.According to the mechanisms of wetting behavior on plant surfaces, a recipe for pesticide formulation can be adjusted with better wettability to reduce loss, improve utilization efficiency, and alleviate adverse effects on the environment.
Screening of the Active Component Promoting Leydig Cell Proliferation from Lepidium meyenii Using HPLC-ESI-MS/MS Coupled with Multivariate Statistical Analysis
Authors: Gao Xiao-chen , Lv Jing-wei , Li Chun-nan , Zhang Nan-xi , Tian Lin-lin , Han Xi-ying , Zhang Hui , Sun Jia-ming
Published: June 2019

Lepidium meyenii is now widely consumed as a functional food and medicinal product, which is known as an enhancer of reproductive health. However, the specific chemical composition and mechanism of action for improving sexual function are unclear. The present study aims at screening and determining the potential compounds, which promote mouse leydig cells (TM3) proliferation. The partial least squares analysis (PLS) was employed to reveal the correlation between common peaks of high performance liquid chromatography (HPLC) fingerprint of L. meyenii and the proliferation activity of TM3. The results suggested that three compounds had good activities on the proliferation of TM3 and promoting testosterone secretion, there were N-benzyl-hexadecanamide, N-benzyl-(9z,12z)-octadecadienamide and N-benzyl-(9z,12z,15z)-octadecatrienamide which might be the potential bioactive markers related to the enhancing sexual ability functions of L. meyenii. The first step in testosterone synthesis is the transport of cholesterol into the mitochondria, and the homeostasis of mitochondrial function is related to cyclophilin D (CypD). In order to expound how bioactive ingredients lead to promoting testosterone secretion, a molecular docking simulation was used for further illustration in the active sites and binding degree of the ligands on CypD. The results indicated there was a positive correlation between the binding energy absolute value and testosterone secretion activity. In addition, in this study it also provided the reference for a simple, quick method to screen the promoting leydig cell proliferation active components in traditional Chinese medicine (TCM).
A Snapshot of Transdermal and Topical Drug Delivery Research in Canada
Authors: Roohnikan Mahdi , Laszlo Elise , Babity Samuel , Brambilla Davide
Published: June 2019

The minimally- or non-invasive delivery of therapeutic agents through the skin has several advantages compared to other delivery routes and plays an important role in medical care routines. The development and refinement of new technologies is leading to a drastic expansion of the arsenal of drugs that can benefit from this delivery strategy and is further intensifying its impact in medicine. Within Canada, as well, a few research groups have worked on the development of state-of-the-art transdermal delivery technologies. Within this short review, we aim to provide a critical overview of the development of these technologies in the Canadian environment.
Logical Problems in Analysis of Analogy
Authors: Woleński Jan
Published: June 2019

The paper discusses some logical problems concerning analogy. The traditional understanding of analogy as proportion (proportion) is inadequate, at least if proportionality is taken in mathematical sense. This situation is clear if we considered various special cases of analogy for instance analogia legis and analogia juris. Since analogy assumes a similarity of analogata (items being or investigated) as analogical, a general analysis of analogical relation must begin with the concept of similarity. It can be defined as possessing a common property. This idea is formalized by devices borrowed from logic and set theory.
Enhanced Production of Anti-PD1 Antibody in CHO Cells through Transient Co-Transfection with Anti-Apoptotic Gene Bcl-xL Combined with Rapamycin
Authors: Li Yunxia , Zhang Xinyu , Wang Lei , Zong Huifang , Yuan Yuan , Han Lei , Li Xi , Xu Chenxiao , Zhang Jingyi , Zhu Jianwei , Zhang Baohong
Published: June 2019

CHO cells are often used to produce monoclonal antibodies in mammalian cell expression systems. In the process of large-scale cell culture, apoptosis is related to cell survival and product quality. Over-expressing an anti-apoptotic gene to delay apoptosis and improve cell growth is one of the strategies for improving productivity of monoclonal antibodies. Autophagy inducer rapamycin can extend the culture duration of CHO cells and affect the yield of antibodies. A method was developed for transient co-transfection of anti-apoptotic genes and genes of interest combined with rapamycin to increase the transient expression of the anti-PD1 antibody. Under the optimal transfection conditions, the combination of Bcl-xL and rapamycin can significantly delay cell apoptosis, inhibit cell proliferation, and prolong cell life-time. As a result, anti-PD1 monoclonal antibody expression levels are increased by more than 2 times.
Narrativism and the Unity of Opposites: Theory, Practice, and Exegesis: A Study of Three Stories from the Talmud
Authors: Rosenak Avinoam , Zinger Sharon Leshem
Published: June 2019

In this article, we pursue a double mission: First, we will demonstrate the unique nature of dynamic group facilitation as it emerges from the concept of the unity of opposites and its relation to situations of conflict, as well as the pedagogical challenges that teachers face in the classroom. This approach underlines the value of a more dialogical and dynamic understanding of the intricate networks of relationships that take place between students and with a teacher at any given moment in a classroom situation. Second, we will examine three Talmudic midrashim that focus on conflict and reconciliation through the lens of facilitation, while casting light on the theology behind the facilitation method and its hermeneutic power. Again, this approach to the interpretation of these texts allows them to emerge as valuable not only to the learning process, but also to the dynamics of interaction that saturate the learning situation. To this end, we will highlight the links and differences between two styles of facilitation—the narrative and the unity of opposites. These links and differences will help us illuminate the similarities and differences between the facilitation processes they employ. Because (1) the notion of exegesis is strongly embedded in narrative theory; (2) theology has deep roots in the concept of the unity of opposites; and (3) both styles address conflict and its resolution, in the second part of this article, we take the insights of the narrative and unity of opposites approaches and juxtapose them as hermeneutic tools for reading three related Talmudic midrashim that focus on conflict and reconciliation. In this way, we hope to exemplify how the different approaches can be applied to the design of the different facilitation styles, both in conflict dialogue groups and as a lens through which we can read these seminal tales that have shaped consciousness, identity, and the attitude towards the culture of debate in Judaism.
Augmented Reality Mapping of Rock Mass Discontinuities and Rockfall Susceptibility Based on Unmanned Aerial Vehicle Photogrammetry
Authors: Zhang Yichi , Yue Pan , Zhang Guike , Guan Tao , Lv Mingming , Zhong Denghua
Published: June 2019

In rockfall hazard management, the investigation and detection of potential rockfall source areas on rock cliffs by remote-sensing-based susceptibility analysis are of primary importance. However, when the rockfall analysis results are used as feedback to the fieldwork, the irregular slope surface morphology makes it difficult to objectively locate the risk zones of hazard maps on the real slopes, and the problem of straightforward on-site visualization of rockfall susceptibility remains a research gap. This paper presents some of the pioneering studies on the augmented reality (AR) mapping of geospatial information from cyberspace within 2D screens to the physical world for on-site visualization, which directly recognizes the rock mass and superimposes corresponding rock discontinuities and rockfall susceptibility onto the real slopes. A novel method of edge-based tracking of the rock mass target for mobile AR is proposed, where the model edges extracted from unmanned aerial vehicle (UAV) structure-from-motion (SfM) 3D reconstructions are aligned with the corresponding actual rock mass to estimate the camera pose accurately. Specifically, the visually prominent edges of dominant structural planes were first explored and discovered to be a robust visual feature of rock mass for AR tracking. The novel approaches of visual-geometric synthetic image (VGSI) and prominent structural plane (Pro-SP) were developed to extract structural planes with identified prominent edges as 3D template models which could provide a pose estimation reference. An experiment verified that the proposed Pro-SP template model could effectively improve the edge tracking performance and quality, and this approach was relatively robust to the changes of sunlight conditions. A case study was carried out on a typical roadcut cliff in the Mentougou District of Beijing, China. The results validate the scalability of the proposed mobile AR strategy, which is applicable and suitable for cliff-scale fieldwork. The results also demonstrate the feasibility, efficiency, and significance of the geoinformation AR mapping methodology for on-site zoning and locating of potential rockfalls, and providing relevant guidance for subsequent detailed site investigation.
Performance of Elephant Herding Optimization and Tree Growth Algorithm Adapted for Node Localization in Wireless Sensor Networks
Authors: Strumberger Ivana , Minovic Miroslav , Tuba Milan , Bacanin Nebojsa
Published: June 2019

Wireless sensor networks, as an emerging paradigm of networking and computing, have applications in diverse fields such as medicine, military, environmental control, climate forecasting, surveillance, etc. For successfully tackling the node localization problem, as one of the most significant challenges in this domain, many algorithms and metaheuristics have been proposed. By analyzing available modern literature sources, it can be seen that the swarm intelligence metaheuristics have obtained significant results in this domain. Research that is presented in this paper is aimed towards achieving further improvements in solving the wireless sensor networks localization problem by employing swarm intelligence. To accomplish this goal, we have improved basic versions of the tree growth algorithm and the elephant herding optimization swarm intelligence metaheuristics and applied them to solve the wireless sensor networks localization problem. In order to determine whether the improvements are accomplished, we have conducted empirical experiments on different sizes of sensor networks ranging from 25 to 150 target nodes, for which distance measurements are corrupted by Gaussian noise. Comparative analysis with other state-of-the-art swarm intelligence algorithms that have been already tested on the same problem instance, the butterfly optimization algorithm, the particle swarm optimization algorithm, and the firefly algorithm, is conducted. Simulation results indicate that our proposed algorithms can obtain more consistent and accurate locations of the unknown target nodes in wireless sensor networks topology than other approaches that have been proposed in the literature.
New Considerations for Collecting Biomechanical Data Using Wearable Sensors: How Does Inclination Influence the Number of Runs Needed to Determine a Stable Running Gait Pattern?
Authors: Ahamed Nizam U. , Benson Lauren C. , Clermont Christian A. , Pohl Andrew J. , Ferber Reed
Published: June 2019

As inertial measurement units (IMUs) are used to capture gait data in real-world environments, guidelines are required in order to determine a ‘typical’ or ‘stable’ gait pattern across multiple days of data collection. Since uphill and downhill running can greatly affect the biomechanics of running gait, this study sought to determine the number of runs needed to establish a stable running pattern during level, downhill, and uphill conditions for both univariate and multivariate analyses of running biomechanical data collected using a single wearable IMU device. Pelvic drop, ground contact time, braking, vertical oscillation, pelvic rotation, and cadence, were recorded from thirty-five recreational runners running in three elevation conditions: level, downhill, and uphill. Univariate and multivariate normal distributions were estimated from differing numbers of runs and stability was defined when the addition of a new run resulted in less than a 5% change in the 2.5 and 97.5 quantiles of the 95% probability density function for each individual runner. This stability point was determined separately for each runner and each IMU variable (univariate and multivariate). The results showed that 2–4 runs were needed to define a stable running pattern for univariate, and 4–5 days were necessary for multivariate analysis across all inclination conditions. Pearson’s correlation coefficients were calculated to cross-validate differing elevation conditions and showed excellent correlations (r = 0.98 to 1.0) comparing the training and testing data within the same elevation condition and good to very good correlations (r = 0.63–0.88) when comparing training and testing data from differing elevation conditions. These results suggest that future research involving wearable technology should collect multiple days of data in order to build reliable and accurate representations of an individual’s stable gait pattern.
Real-Time Detection of Seven Phases of Gait in Children with Cerebral Palsy Using Two Gyroscopes
Authors: Behboodi Ahad , Zahradka Nicole , Wright Henry , Alesi James , Lee Samuel. C. K.
Published: June 2019

A recently designed gait phase detection (GPD) system, with the ability to detect all seven phases of gait in healthy adults, was modified for GPD in children with cerebral palsy (CP). A shank-attached gyroscope sent angular velocity to a rule-based algorithm in LabVIEW to identify the distinct characteristics of the signal. Seven typically developing children (TD) and five children with CP were asked to walk on treadmill at their self-selected speed while using this system. Using only shank angular velocity, all seven phases of gait (Loading Response, Mid-Stance, Terminal Stance, Pre-Swing, Initial Swing, Mid-Swing and Terminal Swing) were reliably detected in real time. System performance was validated against two established GPD methods: (1) force-sensing resistors (GPD-FSR) (for typically developing children) and (2) motion capture (GPD-MoCap) (for both typically developing children and children with CP). The system detected over 99% of the phases identified by GPD-FSR and GPD-MoCap. Absolute values of average gait phase onset detection deviations relative to GPD-MoCap were less than 100 ms for both TD children and children with CP. The newly designed system, with minimized sensor setup and low processing burden, is cosmetic and economical, making it a viable solution for real-time stand-alone and portable applications such as triggering functional electrical stimulation (FES) in rehabilitation systems. This paper verifies the applicability of the GPD system to identify specific gait events for triggering FES to enhance gait in children with CP.
Target Localization Using Double-Sided Bistatic Range Measurements in Distributed MIMO Radar Systems
Authors: Shin Hyuksoo , Chung Wonzoo
Published: June 2019

We develop a novel approach improving existing target localization algorithms for distributed multiple-input multiple-output (MIMO) radars based on bistatic range measurements (BRMs). In the proposed algorithms, we estimate the target position with auxiliary parameters consisting of both the target–transmitter distances and the target–receiver distances (hence, “double-sided”) in contrast to the existing BRM methods. Furthermore, we apply the double-sided approach to multistage BRM methods. Performance improvements were demonstrated via simulations and a limited theoretical analysis was attempted for the ideal two-dimensional case.
The Lightweight Autonomous Vehicle Self-Diagnosis (LAVS) Using Machine Learning Based on Sensors and Multi-Protocol IoT Gateway
Authors: Jeong YiNa , Son SuRak , Lee ByungKwan
Published: June 2019

This paper proposes the lightweight autonomous vehicle self-diagnosis (LAVS) using machine learning based on sensors and the internet of things (IoT) gateway. It collects sensor data from in-vehicle sensors and changes the sensor data to sensor messages as it passes through protocol buses. The changed messages are divided into header information, sensor messages, and payloads and they are stored in an address table, a message queue, and a data collection table separately. In sequence, the sensor messages are converted to the message type of the other protocol and the payloads are transferred to an in-vehicle diagnosis module (In-VDM). The LAVS informs the diagnosis result of Cloud or road side unit(RSU) by the internet of vehicles (IoV) and of drivers by Bluetooth. To design the LAVS, the following two modules are needed. First, a multi-protocol integrated gateway module (MIGM) converts sensor messages for communication between two different protocols, transfers the extracted payloads to the In-VDM, and performs IoV to transfer the diagnosis result and payloads to the Cloud through wireless access in vehicular environment(WAVE). Second, the In-VDM uses random forest to diagnose parts of the vehicle, and delivers the results of the random forest as an input to the neural network to diagnose the total condition of the vehicle. Since the In-VDM uses them for self-diagnosis, it can diagnose a vehicle with efficiency. In addition, because the LAVS converts payloads to a WAVE message and uses IoV to transfer the WAVE messages to RSU or the Cloud, it prevents accidents in advance by informing the vehicle condition of drivers rapidly.
Floor Identification Using Magnetic Field Data with Smartphone Sensors
Authors: Ashraf Imran , Hur Soojung , Shafiq Muhammad , Park Yongwan
Published: June 2019

Floor identification plays a key role in multi-story indoor positioning and localization systems. Current floor identification systems rely primarily on Wi-Fi signals and barometric pressure data. Barometric systems require installation of additional standalone sensors to perform floor identification. Wi-Fi systems, on the other hand, are vulnerable to the dynamic environment and adverse effects of path loss, shadowing, and multipath fading. In this paper, we take advantage of a pervasive magnetic field to compensate for the limitations of these systems. We employ smartphone sensors to make the proposed scheme infrastructure free and cost-effective. We use smartphone magnetic sensors to identify the floors in a multi-story building with improved accuracy. Floor identification is performed with user activities of normal walking, call listening, and phone swinging. Various machine learning techniques are leveraged to identify user activities. Extensive experiments are performed to evaluate the proposed magnetic-data-based floor identification scheme. Additionally, the impact of device heterogeneity on floor identification is investigated using Samsung Galaxy S8, LG G6, and LG G7 smartphones. Research results demonstrate that the magnetic floor identification outperforms barometric and Wi-Fi-enabled floor detection techniques. A floor change module is incorporated to further enhance the accuracy of floor identification.
Increasing Gender Diversity in Higher Education Leadership: The Role of Executive Search Firms
Authors: Manfredi Simonetta , Clayton-Hathway Kate , Cousens Emily
Published: June 2019

Women are under-represented in leadership roles in United Kingdom Higher Education Institutions (HEIs). Existing scholarship focuses on institutional barriers, which include cognitive bias and entrenched homosocial cultures, rather than external factors such as the use of executive search firms (ESFs) in recruitment and selection. Recent research indicates that the use of ESFs is increasing for senior HEI appointments. This analysis offers insights on these firms’ involvement from a gender equality perspective, based on the results from a study that used a ‘virtuous circle’ approach to research and knowledge exchange. The requirement for HEIs to pay ‘due regard’ to equality considerations under the Public Sector Equality Duty provides a framework for analysis. This paper provides new insights on the dynamics within recruitment processes when ESFs are involved and on how a legislative approach can leverage better equality outcomes.