bluuebunny/crossref_metadata_2025 huggingface dataset:
Bletilla striata Polysaccharide Promotes Diabetic Wound Healing Through Inhibition of the NLRP3 Inflammasome
Authors: Zhao Yan , Wang Qibin , Yan Shan , Zhou Jun , Huang Liangyong , Zhu Haitao , Ye Fang , Zhang Yonghong , Chen Lin , Chen Li , Zheng Tao
Published: April 2021

The aim of this study was to evaluate the therapeutic effects of Bletilla striata polysaccharide (BSP) on wound healing in diabetes mellitus (DM) and to explore the underlying mechanisms. DM mouse models were induced by high fat-diet feeding combined with low-dose streptozocin injection. To establish diabetic foot ulcer (DFU) models, DM mice were wounded on the dorsal surface. Subsequently, mice were treated with vehicle or BSP for 12 days and wound healing was monitored. The effects of BSP on the production of interleukin-1β (IL-1β), tumor necrosis factor-α, macrophages infiltration, angiogenesis, the activation of nucleotide-binding and oligomerization (NACHT) domain, leucine-rich repeat (LRR), and pyrin domain (PYD)-containing protein 3 (NLRP3) inflammasome, and insulin sensitivity in wound tissues were subsequently evaluated. Separated- and cultured- bone marrow-derived macrophages (BMDMs) and cardiac microvascular endothelial cells (CMECs) were isolated from mice and used to investigate the effects of BSP on cell viability, reactive oxygen species (ROS) generation, NLRP3 inflammasome activation and insulin sensitivity in vitro following exposure to high glucose (HG). BSP administration accelerated diabetic wound healing, suppressed macrophage infiltration, promoted angiogenesis, suppressed NLRP3 inflammasome activation, decreased IL-1β secretion, and improved insulin sensitivity in wound tissues in DM mice. In vitro, co-treatment with BSP protected against HG-induced ROS generation, NLRP3 inflammasome activation, and IL-1β secretion in BMDMs, and improved cell viability and decreased ROS levels in CMECs. Moreover, in HG exposed BMDMs-CMECs cultures, BSP treatment suppressed NLRP3 inflammasome activation and IL-1β secretion in BMDMs, and improved cell viability and insulin sensitivity in CMECs. Furthermore, treatment with IL-1β almost completely suppressed the beneficial effects of BSP on the NLRP3 inflammasome, IL-1β secretion, and insulin sensitivity in HG-treated BMDMs-CMECs. BSP promotes DFU healing through inhibition of the HG-activated NLRP3 inflammasome.
Breaching the Delivery Barrier: Chemical and Physical Airway Epithelium Disruption Strategies for Enhancing Lentiviral-Mediated Gene Therapy
Authors: McCarron Alexandra , Farrow Nigel , Cmielewski Patricia , Knight Emma , Donnelley Martin , Parsons David
Published: April 2021

The lungs have evolved complex physical, biological and immunological defences to prevent foreign material from entering the airway epithelial cells. These mechanisms can also affect both viral and non-viral gene transfer agents, and significantly diminish the effectiveness of airway gene-addition therapies. One strategy to overcome the physical barrier properties of the airway is to transiently disturb the integrity of the epithelium prior to delivery of the gene transfer vector. In this study, chemical (lysophosphatidylcholine, LPC) and physical epithelium disruption using wire abrasion were compared for their ability to improve airway-based lentiviral (LV) vector mediated transduction and reporter gene expression in rats. When luciferase expression was assessed at 1-week post LV delivery, LPC airway conditioning significantly enhanced gene expression levels in rat lungs, while a long-term assessment in a separate cohort of rats at 12 months revealed that LPC conditioning did not improve gene expression longevity. In rats receiving physical perturbation to the trachea prior to gene delivery, significantly higher LacZ gene expression levels were found when compared to LPC-conditioned or LV-only control rats when evaluated 1-week post gene transfer. This proof-of-principle study has shown that airway epithelial disruption strategies based on physical perturbation substantially enhanced LV-mediated airway gene transfer in the trachea.
First Evaluation of a Newly Constructed Underwater Pulse Oximeter for Use in Breath-Holding Activities
Authors: Mulder Eric , Schagatay Erika , Sieber Arne
Published: April 2021

Studying risk factors in freediving, such as hypoxic blackout, requires development of new methods to enable remote underwater monitoring of physiological variables. We aimed to construct and evaluate a new water- and pressure proof pulse oximeter for use in freediving research. The study consisted of three parts: (I) A submersible pulse oximeter (SUB) was developed on a ruggedized platform for recording of physiological parameters in challenging environments. Two MAX30102 sensors were used to record plethysmograms, and included red and infra-red emitters, diode drivers, photodiode, photodiode amplifier, analog to digital converter, and controller. (II) We equipped 20 volunteers with two transmission pulse oximeters (TPULS) and SUB to the fingers. Arterial oxygen saturation (SpO2) and heart rate (HR) were recorded, while breathing room air (21% O2) and subsequently a hypoxic gas (10.7% O2) at rest in dry conditions. Bland-Altman analysis was used to evaluate bias and precision of SUB relative to SpO2 values from TPULS. (III) Six freedivers were monitored with one TPULS and SUB placed at the forehead, during a maximal effort immersed static apnea. For dry baseline measurements (n = 20), SpO2 bias ranged between −0.8 and −0.6%, precision between 1.0 and 1.5%; HR bias ranged between 1.1 and 1.0 bpm, precision between 1.4 and 1.9 bpm. For the hypoxic episode, SpO2 bias ranged between −2.5 and −3.6%, precision between 3.6 and 3.7%; HR bias ranged between 1.4 and 1.9 bpm, precision between 2.0 and 2.1 bpm. Freedivers (n = 6) performed an apnea of 184 ± 53 s. Desaturation- and resaturation response time of SpO2 was approximately 15 and 12 s shorter in SUB compared to TPULS, respectively. Lowest SpO2 values were 76 ± 10% for TPULS and 74 ± 13% for SUB. HR traces for both pulse oximeters showed similar patterns. For static apneas, dropout rate was larger for SUB (18%) than for TPULS (<1%). SUB produced similar SpO2 and HR values as TPULS, both during normoxic and hypoxic breathing (n = 20), and submersed static apneas (n = 6). SUB responds more quickly to changes in oxygen saturation when sensors were placed at the forehead. Further development of SUB is needed to limit signal loss, and its function should be tested at greater depth and lower saturation.
Audiomotor Temporal Recalibration Modulates Decision Criterion of Self-Agency but Not Perceptual Sensitivity
Authors: Sugano Yoshimori
Published: April 2021

Exposure to delayed sensory feedback changes perceived simultaneity between action and feedback [temporal recalibration (TR)] and even modulates the sense of agency (SoA) over the feedback. To date, however, it is not clear whether the modulation of SoA by TR is caused by a change in perceptual sensitivity or decision criterion of self-agency. This experimental research aimed to tease apart these two by applying the signal detection theory (SDT) to the agency judgment over auditory feedback after voluntary action. Participants heard a short sequence of tone pips with equal inter-onset intervals, and they reproduced it by pressing a computer mouse. The delay of each tone pip after the mouse press was manipulated as 80 (baseline) or 180 ms (delayed). Subsequently, the participants reproduced it, in which the delay was fixed at 80 ms and there was a 50% chance that the computer took over the control of the tone pips from the participants. The participants’ task was to discriminate who controlled the tone pips and to judge synchrony between tone pips and mouse presses. Results showed that the modulation of the SoA by the TR is caused by a shift in the decision criterion but not in the perceptual sensitivity of agency.
Mechanisms of Cultural Evolution in the Songs of Wild Bird Populations
Authors: Williams Heather
Published: April 2021

Young songbirds draw the source material for their learned songs from parents, peers, and unrelated adults, as well as from innovation. These learned songs are used for intraspecific communication, and have well-documented roles for such functions as territory maintenance and mate attraction. The songs of wild populations differ, forming local “dialects” that may shift over time, suggesting that cultural evolution is at work. Recent work has focused on the mechanisms responsible for the cultural evolution of bird songs within a population, including drift, learning biases (such as conformity and rare-form copying), and selection (including sexual selection). In many songs or song repertoires, variability is partitioned, with some songs or song segments being stable and consistent, while others vary within the population and across time, and still others undergo population-wide transitions over time. This review explores the different mechanisms that shape the cultural evolution of songs in wild populations, with specific reference to a long-term investigation of a single population of philopatric Savannah sparrows. Males learn a single four-segment song during their 1st year and sing the same song thereafter. Within this song, the buzz segment is a population marker, and may be stable for decades – variant forms occur but eventually disappear. In contrast, the middle segment is highly variable both within the population and over time; changes in relative prevalence of different forms may be due to cultural drift or a rare-form learning bias. Within the introductory segment, a high note cluster was replaced by a click train between 1982 and 2010, following an S-shaped trajectory characteristic of both selective sweeps in population genetics and the replacement of one form by another in human language. In the case of the Savannah sparrows, this replacement may have been due to sexual selection. In subsequent generations, the number of clicks within trains increased, a form of cultural directional selection. In contrast to the narrowing of a trait's range during directional selection in genetic systems, variation in the number of clicks in a train increased as the mean value shifted because improvisation during song learning allowed the range of the trait to expand. Thus, in the single short song of the Savannah sparrow, at least four different mechanisms appear to contribute to three different types of cultural evolutionary outcomes. In the future, it will be import to explore the conditions that favor the application of specific (and perhaps conditional) learning rules, and studies such as the ongoing song seeding experiment in the Kent Island Savannah sparrow population will help in understanding the mechanisms that promote or repress changes in a population's song.
Progressive Reduction of Iconic Gestures Contributes to School-Aged Children’s Increased Word Production
Authors: Mertens Ulrich J. , Rohlfing Katharina J.
Published: April 2021

The economic principle of communication, according to which successful communication can be reached by least effort, has been studied for verbal communication. With respect to nonverbal behavior, it implies that forms of iconic gestures change over the course of communication and become reduced in the sense of less pronounced. These changes and their effects on learning are currently unexplored in relevant literature. Addressing this research gap, we conducted a word learning study to test the effects of changing gestures on children’s slow mapping. We applied a within-subject design and tested 51 children, aged 6.7 years (SD = 0.4), who learned unknown words from a story. The storyteller acted on the basis of two conditions: In one condition, in which half of the target words were presented, the story presentation was enhanced with progressively reduced iconic gestures (PRG); in the other condition, half of the target words were accompanied by fully executed iconic gestures (FEG). To ensure a reliable gesture presentation, children were exposed to a recorded person telling a story in both conditions. We tested the slow mapping effects on children’s productive and receptive word knowledge three minutes as well as two to three days after being presented the story. The results suggest that children’s production of the target words, but not their understanding thereof, was enhanced by PRG.
Body Image Dissatisfaction and Impulse Buying: A Moderated Mediation Model
Authors: Cai Zhihui , Gui Yang , Wang Dandan , Yang Han , Mao Peipei , Wang Zhikeng
Published: April 2021

This study investigated the mediating roles of self-acceptance and self-esteem in the relationship between body image dissatisfaction and impulse buying, and tested the moderating effect of gender on the relationships. A sample of 374 college students and graduate students (33.4% male, 66.6% female) participated in the study. Results revealed that (a) body image dissatisfaction positively associated with impulse buying; (b) self-esteem plays a mediating role between body image dissatisfaction and impulsive buying; (c) a serial indirect pathway (i.e., Body image dissatisfaction → self-acceptance → self-esteem → impulse buying) emerged; (d) the mediation path from self-acceptance to self-esteem was stronger for female than that from male. The results underscore the importance of identifying the mechanisms that moderate the mediated path between body image dissatisfaction and impulse buying among students. These findings point to the potential implications about how to reduce impulse buying through improving body image satisfaction.
Improving Care for Deinstitutionalized People With Mental Disorders: Experiences of the Use of Knowledge Translation Tools
Authors: Fulone Izabela , Barreto Jorge Otavio Maia , Barberato-Filho Silvio , Bergamaschi Cristiane de Cássia , Silva Marcus Tolentino , Lopes Luciane Cruz
Published: April 2021

Background: The deinstitutionalization process is complex, long-term and many countries fail to achieve progress and consolidation. Informing decision-makers about appropriate strategies and changes in mental health policies can be a key factor for it. This study aimed to develop an evidence brief to summarize the best available evidence to improve care for deinstitutionalized patients with severe mental disorders in the community.

Methods: We used the SUPPORT (Supporting Policy Relevant Reviews and Trials) tools to elaborate the evidence brief and to organize a policy dialogue with 24 stakeholders. A systematic search was performed in 10 electronic databases and the methodological quality of systematic reviews (SRs) was assessed by AMSTAR 2.

Results: Fifteen SRs were included (comprising 378 studies and 69,736 participants), of varying methodological quality (3 high-quality SRs, 2 moderate-quality SRs, 7 low-quality SRs, 3 critically low SRs). Six strategies were identified: (i). Psychoeducation; (ii). Anti-stigma programs, (iii). Intensive case management; (iv). Community mental health teams; (v). Assisted living; and (vi). Interventions for acute psychiatric episodes. They were associated with improvements on a global status, satisfaction with the service, reduction on relapse, and hospitalization. Challenges to implementation of any of them included: stigma, the shortage of specialized human resources, limited political and budgetary support.

Conclusions: These strategies could guide future actions and policymaking to improve mental health outcomes.
Problematic Use of the Internet in Subjects With Bipolar Disorder: Relationship With Posttraumatic Stress Symptoms
Authors: Carmassi Claudia , Bertelloni Carlo Antonio , Cordone Annalisa , Dell'Oste Valerio , Pedrinelli Virginia , Barberi Filippo Maria , Massimetti Enrico , Bui Eric , Dell'Osso Lliliana
Published: April 2021

Background: Literature shows a high risk for problematic use of the Internet (PUI) in mood disorders, especially in bipolar disorder (BD). In subjects with BD, traumatic events and posttraumatic stress disorder (PTSD) are related to alcohol or substance use disorder, as well as to gambling disorder. However, little is known about the possible association between traumatic exposure and PUI. The present study was aimed at examining the relationship between PUI and trauma exposure, besides PTSD symptoms, in subjects with BD.

Methods: A sample of 113 subjects with BD was screened to putative PUI. Furthermore, they completed the Trauma and Loss Spectrum Self-Report (TALS-SR) to assess traumatic events and posttraumatic stress symptoms.

Results: Twenty-four subjects (21.2%) reported putative PUI. Subjects with putative PUI presented significantly higher scores in the TALS-SR domains Potentially Traumatic Events, Re-experiencing, Maladaptive coping, and Arousal, as well as in the TALS-SR total score. In a logistic regression model, a positive association emerged between Potentially Traumatic Events and Arousal TALS-SR domains and putative PUI.

Conclusion: One in five patients with BD screened positive for PUI. A significant association between PUI and lifetime traumatic events as well as PTSD symptoms emerged, highlighting the relevance of the comorbidity between PTSD and PUI in subjects with BD.
A Framework to Assess the Citation Performance of Complex Innovation Systems
Authors: Ronda-Pupo Guillermo Armando
Published: April 2021

Scientometric indicators are useful to evaluate the relevance of scientific research, to prepare rankings, and to evaluate and inform research policies. That is why the choice of appropriate indicators is a matter of primary concern. This article aims to introduce a framework to decide the appropriate type of indicator for assessing the citation-based performance of complex innovation systems. The framework is two-fold: First, it brings the methodology to decide when the use of standard average based indicators is granted, and when scale-invariant indicators are mandatory. Second, it provides the procedures to build scale-invariant indicators to assess the relative impact of complex innovation systems. The framework is validated empirically through the evaluation of the relative impact of the Chilean science system in 2017. The result suggests that the Chilean science system has characteristics of a complex innovation system such as the distribution of citations fits to a power law with an exponential cutoff −2.77±0.09 and a power-law correlation between the size of the system and its impact 1.29±0.11. Furthermore, the framework shows to be efficient to compare fields of vastly different sizes.
The Effect of Footwear, Running Speed, and Location on the Validity of Two Commercially Available Inertial Measurement Units During Running
Authors: Napier Christopher , Willy Richard W. , Hannigan Brett C. , McCann Ryan , Menon Carlo
Published: April 2021

Introduction: Most running-related injuries are believed to be caused by abrupt changes in training load, compounded by biomechanical movement patterns. Wearable technology has made it possible for runners to quantify biomechanical loads (e.g., peak positive acceleration; PPA) using commercially available inertial measurement units (IMUs). However, few devices have established criterion validity. The aim of this study was to assess the validity of two commercially available IMUs during running. Secondary aims were to determine the effect of footwear, running speed, and IMU location on PPA.

Materials and Methods: Healthy runners underwent a biomechanical running analysis on an instrumented treadmill. Participants ran at their preferred speed in three footwear conditions (neutral, minimalist, and maximalist), and at three speeds (preferred, +10%, −10%) in the neutral running shoes. Four IMUs were affixed at the distal tibia (IMeasureU-Tibia), shoelaces (RunScribe and IMeasureU-Shoe), and insole (Plantiga) of the right shoe. Pearson correlations were calculated for average vertical loading rate (AVLR) and PPA at each IMU location.

Results: The AVLR had a high positive association with PPA (IMeasureU-Tibia) in the neutral and maximalist (r = 0.70–0.72; p ≤ 0.001) shoes and in all running speed conditions (r = 0.71–0.83; p ≤ 0.001), but low positive association in the minimalist (r = 0.47; p < 0.05) footwear condition. Conversely, the relationship between AVLR and PPA (Plantiga) was high in the minimalist (r = 0.75; p ≤ 0.001) condition and moderate in the neutral (r = 0.50; p < 0.05) and maximalist (r = 0.57; p < 0.01) footwear. The RunScribe metrics demonstrated low to moderate positive associations (r = 0.40–0.62; p < 0.05) with AVLR across most footwear and speed conditions.

Discussion: Our findings indicate that the commercially available Plantiga IMU is comparable to a tibia-mounted IMU when acting as a surrogate for AVLR. However, these results vary between different levels of footwear and running speeds. The shoe-mounted RunScribe IMU exhibited slightly lower positive associations with AVLR. In general, the relationship with AVLR improved for the RunScribe sensor at slower speeds and improved for the Plantiga and tibia-mounted IMeasureU sensors at faster speeds.
Exercise-Induced Hypoxemia in Endurance Athletes: Consequences for Altitude Exposure
Authors: Durand Fabienne , Raberin Antoine
Published: April 2021

Exercise-induced hypoxemia (EIH) is well-described in endurance-trained athletes during both maximal and submaximal exercise intensities. Despite the drop in oxygen (O2) saturation and provided that training volumes are similar, athletes who experience EIH nevertheless produce the same endurance performance in normoxia as athletes without EIH. This lack of a difference prompted trainers to consider that the phenomenon was not relevant to performance but also suggested that a specific adaptation to exercise is present in EIH athletes. Even though the causes of EIH have been extensively studied, its consequences have not been fully characterized. With the development of endurance outdoor activities and altitude/hypoxia training, athletes often train and/or compete in this stressful environment with a decrease in the partial pressure of inspired O2(due to the drop in barometric pressure). Thus, one can reasonably hypothesize that EIH athletes can specifically adapt to hypoxemic episodes during exercise at altitude. Although our knowledge of the interactions between EIH and acute exposure to hypoxia has improved over the last 10 years, many questions have yet to be addressed. Firstly, endurance performance during acute exposure to altitude appears to be more impaired in EIH vs. non-EIH athletes but the corresponding physiological mechanisms are not fully understood. Secondly, we lack information on the consequences of EIH during chronic exposure to altitude. Here, we (i) review research on the consequences of EIH under acute hypoxic conditions, (ii) highlight unresolved questions about EIH and chronic hypoxic exposure, and (iii) suggest perspectives for improving endurance training.
Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized Cows
Authors: Macedo Gustavo Guerino , Batista Emiliana de Oliveira Santana , Santos Gustavo Martins Gomes dos , D’Occhio Michael J. , Baruselli Pietro Sampaio
Published: April 2021

The present study examined whether priming with estradiol benzoate (EB) for 12 h increased both the peak and duration of LH release in response to kisspeptin (KISS1, KP) in cows. In a Latin square design, ovariectomized Nelore cows (n = 8) received: Control, i.m. 4 mL of 0.9% saline; KP, i.m. 4 mg murine KISS1-10; EBKP, i.m. 4 mg KISS1-10 + i.m. 2 mg EB simultaneously; EB12KP, i.m. 4 mg KISS1-10 + i.m. 2 mg EB 12 h before KISS1-10. Concentrations of LH were determined in blood samples obtained at time 0 (treatment), 20, 40, 60, 90, 120, 150, 180, 210 and 270 min. Concentrations of LH were analyzed by Proc GLIMMIX for repeated measures. In case of significance, the adjusted Tukey test was used to test for differences among treatments. GraphPad 8.0 PRISM® was used to determine the area under the LH-response curve (AUC) after injection of KISS1-10. Plasma LH remained relatively constant throughout sampling after treatment with saline. The peak in LH after injection of KISS1-10 occurred at 20 min in Groups KP and EBKP and at 40 min in Group EB12KP. The peak LH response (∆LH, ng/mL) was greater (p < 0.01) in Group EB12KP (5.6 ± 0.9) than in Groups KP (2.4 ± 0.9) and EBKP (3.5 ± 0.9), which did not differ. AUC (LH ng/mL*min) was greater (p = 0.02) in Group EB12KP (439 ± 73) than in Groups KP (176 ± 73) and EBKP (241 ± 73), with the latter two groups not differing. The findings indicated that 12 h priming with EB increased both the peak and duration of the LH response to treatment with KISS1. The incorporation of EB priming and KISS1 could improve the efficiency of estrus synchronization with fixed-time AI in cows. This would have an important practical application in assisted breeding in beef and dairy cattle.
Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes
Authors: El-Magd Mohammed A. , Fathy Aziza , Kahilo Khaled A. , Saleh Ayman A. , El Sheikh Ahmed I. , AL-Shami Salah , El-Komy Shymaa M.
Published: April 2021

Prolactin (PRL) and its receptor (PRLR) were considered as potential genetic markers for milk production and quality traits in cattle. However, little information is available regarding PRLR genetic diversity and association studies with milk traits in Egyptian water buffaloes. Therefore, the present study was conducted to search for mutations in PRLR and determine their associations with milk performance in these animals. Exon3 (E3) and E10 of PRLR were screened for polymorphisms using single strand conformation polymorphism (SSCP) and sequencing in 400 buffaloes. The associations between haplotypes and milk production (fat%, protein%, lactose%, and solid%) traits as well as mRNA and protein levels of PRL and PRLR were studied. Two single nucleotide polymorphisms (SNPs) in E10 were detected: g.11685G>A (p.Ala494Thr) and g.11773T>C (p.Val523Aal). The G and T alleles were wild (ancestral) alleles, while the A and C alleles were mutant alleles. These SNPs resulted in four haplotypes; AC, AT, GC, and GT. Buffaloes with wild GT haplotypes showed significantly higher milk yield, fat% and protein%, mRNA and protein levels of PRL and PRLR in milk somatic cells than other animals. Animals carrying mutant AC haplotype had inferior milk traits and lowest levels of associated mRNAs and proteins. With these results, we could conclude that the selection of buffaloes with wild GT haplotypes for g.11685G>A and g.11773T>C SNPs of the PRLR gene might improve the milk production traits of Egyptian water buffaloes.
The Specific Immune Response after Vaccination against Neonatal Calf Diarrhoea Differs between Apparent Similar Vaccines in a Case Study
Authors: Gonzalez Román , Elvira Laura , Carbonell Carlos , Vertenten Geert , Fraile Lorenzo
Published: April 2021

Neonatal calf diarrhoea (NCD) is a major health challenge with a negative impact on farm profitability, calf welfare and antimicrobial use. Neonatal calves are particularly sensitive to enteric infections. Thus, a key point for prevention is minimising infectious pressure and maximising specific immune responses. An amount of 120 dams not previously vaccinated against NCD were randomly allocated to one of three study groups: negative control versus two vaccinated groups (A and B). In the control group, the average level of antibodies was significantly low for both BoCV and ETEC (15.6 and 13.9% in the colostrum samples, respectively), demonstrating the importance of dam vaccination. Indeed, the level of specific immunity was significantly increased for BoCV and ETEC with dam vaccination using both one-shot vaccines versus the control group. Moreover, the statistical analysis revealed a significantly higher level of antibodies for BoCV and ETEC in colostrum samples in vaccine A versus vaccine B and the control group. In accordance, the calf serum demonstrated a significantly higher level and greater homogeneity of antibodies against BoCV and ETEC in the Vaccine A group versus other experimental groups (p < 0.05). In conclusion, this study demonstrated a different specific immune response for the pathogens depending on the vaccine used to control NCD in cows.
Anthropogenic Influences on Distance Traveled and Vigilance Behavior and Stress-Related Endocrine Correlates in Free-Roaming Giraffes
Authors: Scheijen Ciska P. J. , van der Merwe Sean , Ganswindt Andre , Deacon Francois
Published: April 2021

Giraffes are an important tourist attraction, and human presence to wildlife is increasing. This has an impact on an animal’s behavior and its endocrine correlates. Studies on other species show alterations in movement patterns, vigilance, and stress-related hormone levels in the presence of humans. Limited information is available on how anthropogenic activities alter giraffe’s behavior, social structure, and related endocrine parameters. The purpose of this study was to obtain insight into anthropogenic influences on giraffe’s behavior and adrenal activity. We used GPS devices mounted onto giraffes to compare the distance walked in the presence or absence of human observers. We also conducted behavioral observations to assess their vigilance and collected fecal samples to analyze their fecal glucocorticoid metabolite (fGCM) concentrations. Giraffes walked significantly further distances in the presence of humans, but the cumulative time that observers were present decreased the hourly distance walked with an observer present, suggesting that the giraffes were becoming habituated. The number of observers present significantly increased the percentage of time spent on observing an observer as well as the number of unhabituated individuals present in the herd. The percentage of time spent observing a human observer did not decrease with the increase of habituation. Last, fGCM concentrations increased with human presence but decreased when individuals became habituated to human presence. More research is needed to understand the effect of anthropogenic influences in different scenarios (e.g., tourism, vehicles, hunting, etc.).
The Historical Trend of Air Pollution and Its Impact on Human Health in Campania Region (Italy)
Authors: Toscano Domenico , Murena Fabio
Published: April 2021

The Campania region covers an area of about 13,590 km2 with 5.8 million residents. The area suffers from several environmental issues due to urbanization, the presence of industries, wastewater treatment, and solid waste management concerns. Air pollution is one of the most relevant environmental troubles in the Campania region, frequently exceeding the limit values established by European directives. In this paper, airborne pollutant concentration data measured by the regional air quality network from 2003 to 2019 are collected to individuate the historical trends of nitrogen dioxide (NO2), coarse and fine particulate matter with aerodynamic diameters smaller than 10 μm (PM10) and 2.5 μm (PM2.5), and ozone (O3) through the analysis of the number of exceedances of limit values per year and the annual average concentration. Information on spatial variability and the effect of the receptor category is obtained by lumping together data belonging to the same province or category. To obtain information on the general air quality rather than on single pollutants, the European Air Quality Index (EU-AQI) is also evaluated. A special focus is dedicated to the effect of deep street canyons on air quality, since they are very common in the urban areas in Campania. Finally, the impact of air pollution from 2003 to 2019 on human health is also analyzed using the software AIRQ+.
Induction of Apoptosis, Inhibition of MCL-1, and VEGF-A Expression Are Associated with the Anti-Cancer Efficacy of Magnolol Combined with Regorafenib in Hepatocellular Carcinoma
Authors: Chen Cheng-Hsien , Hsu Fei-Ting , Chen Wei-Lung , Chen Jiann-Hwa
Published: April 2021

While regorafenib was approved for the treatment of advanced HCC in 2017, with a partial response and survival benefit; other combination agents to facilitate the efficacy of regorafenib still need to be explored. Magnolol is a potential natural anti-tumor compound for many types of cancers. Combination indexes calculated on the basis of both in vitro and in vivo models have indicated a synergistic effect of the combination of regorafenib and magnolol. The overexpression of the VEGF-A protein significantly diminished regorafenib’s inhibition of cell viability, while the transient knockdown of VEGF-A by siRNA effectively sensitized HCC cells to regorafenib. In addition, the inhibition of MCL-1 by siRNA combined with regorafenib allowed for a significantly greater inhibition of cell growth, compared to regorafenib alone. A lower protein expression level for VEGF-A and MCL-1 was found for the combination treatment of HCC in vitro and in vivo. A superior metastasis inhibition was also found in the combination group, as compared to the single-treatment groups, using a transwell assay, wound healing assay, and Western blotting. The caspase-dependent and -independent and DNA damage effects, as determined by flow cytometry and a comet assay, were increased by the combination therapy. Taken together, magnolol sensitized HCC to regorafenib, which was correlated with the reduction of VEGF-A and MCL-1 and the induction of apoptosis.
MiR-182-5p and miR-375-3p Have Higher Performance Than PSA in Discriminating Prostate Cancer from Benign Prostate Hyperplasia
Authors: Abramovic Irena , Vrhovec Borna , Skara Lucija , Vrtaric Alen , Nikolac Gabaj Nora , Kulis Tomislav , Stimac Goran , Ljiljak Dejan , Ruzic Boris , Kastelan Zeljko , Kruslin Bozo , Bulic-Jakus Floriana , Ulamec Monika , Katusic-Bojanac Ana , Sincic Nino
Published: April 2021

Prostate cancer (PCa) is the most commonly diagnosed neoplasm among men. Since it often resembles benign prostate hyperplasia (BPH), biomarkers with a higher differential value than PSA are required. Epigenetic biomarkers in liquid biopsies, especially miRNA, could address this challenge. The absolute expression of miR-375-3p, miR-182-5p, miR-21-5p, and miR-148a-3p were quantified in blood plasma and seminal plasma of 65 PCa and 58 BPH patients by digital droplet PCR. The sensitivity and specificity of these microRNAs were determined using ROC curve analysis. The higher expression of miR-182-5p and miR-375-3p in the blood plasma of PCa patients was statistically significant as compared to BPH (p = 0.0363 and 0.0226, respectively). Their combination achieved a specificity of 90.2% for predicting positive or negative biopsy results, while PSA cut-off of 4 µg/L performed with only 1.7% specificity. In seminal plasma, miR-375-3p, miR-182-5p, and miR-21-5p showed a statistically significantly higher expression in PCa patients with PSA >10 µg/L compared to ones with PSA ≤10 µg/L. MiR-182-5p and miR-375-3p in blood plasma show higher performance than PSA in discriminating PCa from BPH. Seminal plasma requires further investigation as it represents an obvious source for PCa biomarker identification.
A Special View of What Was Almost Forgotten: p38δ MAPK
Authors: Anton Débora Bublitz , Ducati Rodrigo Gay , Timmers Luís Fernando Saraiva Macedo , Laufer Stefan , Goettert Márcia Inês
Published: April 2021

The p38δ mitogen-activated protein kinase is an important signal transduction enzyme. p38δ has recently emerged as a drug target due to its tissue-specific expression patterns and its critical roles in regulation of cellular processes related to cancer and inflammatory diseases, such as cell proliferation, cell migration, apoptosis, and inflammatory responses. However, potent and specific p38δ inhibitors have not been defined so far. Moreover, in cancer disease, p38δ appears to act as a tumor suppressor or tumor promoter according to cancer and cell type studied. In this review, we outline the current understanding of p38δ roles in each cancer type, to define whether it is possible to delineate new cancer therapies based on small-molecule p38δ inhibitors. We also highlight recent advances made in the design of molecules with potential to inhibit p38 isoforms and discuss structural approaches to guide the search for p38δ inhibitors.
Nanoparticle Delivered Anti-miR-141-3p for Stroke Therapy
Authors: Dhuri Karishma , Vyas Rutesh N. , Blumenfeld Leslie , Verma Rajkumar , Bahal Raman
Published: April 2021

Ischemic stroke and factors modifying ischemic stroke responses, such as social isolation, contribute to long-term disability worldwide. Several studies demonstrated that the aberrant levels of microRNAs contribute to ischemic stroke injury. In prior studies, we established that miR-141-3p increases after ischemic stroke and post-stroke isolation. Herein, we explored two different anti-miR oligonucleotides; peptide nucleic acid (PNAs) and phosphorothioates (PS) for ischemic stroke therapy. We used US FDA approved biocompatible poly (lactic-co-glycolic acid) (PLGA)-based nanoparticle formulations for delivery. The PNA and PS anti-miRs were encapsulated in PLGA nanoparticles by double emulsion solvent evaporation technique. All the formulated nanoparticles showed uniform morphology, size, distribution, and surface charge density. Nanoparticles also exhibited a controlled nucleic acid release profile for 48 h. Further, we performed in vivo studies in the mouse model of ischemic stroke. Ischemic stroke was induced by transient (60 min) occlusion of middle cerebral artery occlusion followed by a reperfusion for 48 or 72 h. We assessed the blood-brain barrier permeability of PLGA NPs containing fluorophore (TAMRA) anti-miR probe after systemic delivery. Confocal imaging shows uptake of fluorophore tagged anti-miR in the brain parenchyma. Next, we evaluated the therapeutic efficacy after systemic delivery of nanoparticles containing PNA and PS anti-miR-141-3p in mice after stroke. Post-treatment differentially reduced both miR-141-3p levels in brain tissue and infarct injury. We noted PNA-based anti-miR showed superior efficacy compared to PS-based anti-miR. Herein, we successfully established that nanoparticles encapsulating PNA or PS-based anti-miRs-141-3p probes could be used as a potential treatment for ischemic stroke.
The Role of Electric Pressure/Stress Suppressing Pinhole Defect on Coalescence Dynamics of Electrified Droplet
Authors: Lee Jaehyun , Esmaili Ehsan , Kang Giho , Seong Baekhoon , Kang Hosung , Kim Jihoon , Jung Sunghwan , Kim Hyunggun , Byun Doyoung
Published: April 2021

The dimple occurs by sudden pressure inversion at the droplet’s bottom interface when a droplet collides with the same liquid-phase or different solid-phase. The air film entrapped inside the dimple is a critical factor affecting the sequential dynamics after coalescence and causing defects like the pinhole. Meanwhile, in the coalescence dynamics of an electrified droplet, the droplet’s bottom interfaces change to a conical shape, and droplet contact the substrate directly without dimple formation. In this work, the mechanism for the dimple’s suppression (interfacial change to conical shape) was studied investigating the effect of electric pressure. The electric stress acting on a droplet interface shows the nonlinear electric pressure adding to the uniform droplet pressure. This electric stress locally deforms the droplet’s bottom interface to a conical shape and consequentially enables it to overcome the air pressure beneath the droplet. The electric pressure, calculated from numerical tracking for interface and electrostatic simulation, was at least 108 times bigger than the air pressure at the center of the coalescence. This work helps toward understanding the effect of electric stress on droplet coalescence and in the optimization of conditions in solution-based techniques like printing and coating.
A Review on Machine Learning Approaches for Network Malicious Behavior Detection in Emerging Technologies
Authors: Rabbani Mahdi , Wang Yongli , Khoshkangini Reza , Jelodar Hamed , Zhao Ruxin , Bagheri Baba Ahmadi Sajjad , Ayobi Seyedvalyallah
Published: April 2021

Network anomaly detection systems (NADSs) play a significant role in every network defense system as they detect and prevent malicious activities. Therefore, this paper offers an exhaustive overview of different aspects of anomaly-based network intrusion detection systems (NIDSs). Additionally, contemporary malicious activities in network systems and the important properties of intrusion detection systems are discussed as well. The present survey explains important phases of NADSs, such as pre-processing, feature extraction and malicious behavior detection and recognition. In addition, with regard to the detection and recognition phase, recent machine learning approaches including supervised, unsupervised, new deep and ensemble learning techniques have been comprehensively discussed; moreover, some details about currently available benchmark datasets for training and evaluating machine learning techniques are provided by the researchers. In the end, potential challenges together with some future directions for machine learning-based NADSs are specified.
A Study of the Effects of the COVID-19 Pandemic on the Experience of Back Pain Reported on Twitter® in the United States: A Natural Language Processing Approach
Authors: Fiok Krzysztof , Karwowski Waldemar , Gutierrez Edgar , Saeidi Maham , Aljuaid Awad M. , Davahli Mohammad Reza , Taiar Redha , Marek Tadeusz , Sawyer Ben D.
Published: April 2021

The COVID-19 pandemic has changed our lifestyles, habits, and daily routine. Some of the impacts of COVID-19 have been widely reported already. However, many effects of the COVID-19 pandemic are still to be discovered. The main objective of this study was to assess the changes in the frequency of reported physical back pain complaints reported during the COVID-19 pandemic. In contrast to other published studies, we target the general population using Twitter as a data source. Specifically, we aim to investigate differences in the number of back pain complaints between the pre-pandemic and during the pandemic. A total of 53,234 and 78,559 tweets were analyzed for November 2019 and November 2020, respectively. Because Twitter users do not always complain explicitly when they tweet about the experience of back pain, we have designed an intelligent filter based on natural language processing (NLP) to automatically classify the examined tweets into the back pain complaining class and other tweets. Analysis of filtered tweets indicated an 84% increase in the back pain complaints reported in November 2020 compared to November 2019. These results might indicate significant changes in lifestyle during the COVID-19 pandemic, including restrictions in daily body movements and reduced exposure to routine physical exercise.
Implementation of an Electronic National Early Warning System to Decrease Clinical Deterioration in Hospitalized Patients at a Tertiary Medical Center
Authors: Wu Chieh-Liang , Kuo Chen-Tsung , Shih Sou-Jen , Chen Jung-Chen , Lo Ying-Chih , Yu Hsiu-Hui , Huang Ming-De , Sheu Wayne Huey-Herng , Liu Shih-An
Published: April 2021

The National Early Warning Score (NEWS) is an early warning system that predicts clinical deterioration. The impact of the NEWS on the outcome of healthcare remains controversial. This study was conducted to evaluate the effectiveness of implementing an electronic version of the NEWS (E-NEWS), to reduce unexpected clinical deterioration. We developed the E-NEWS as a part of the Health Information System (HIS) and Nurse Information System (NIS). All adult patients admitted to general wards were enrolled into the current study. The “adverse event” (AE) group consisted of patients who received cardiopulmonary resuscitation (CPR), were transferred to an intensive care unit (ICU) due to unexpected deterioration, or died. Patients without AE were allocated to the control group. The development of the E-NEWS was separated into a baseline (October 2018 to February 2019), implementation (March to August 2019), and intensive period (September. to December 2019). A total of 39,161 patients with 73,674 hospitalization courses were collected. The percentage of overall AEs was 6.06%. Implementation of E-NEWS was associated with a significant decrease in the percentage of AEs from 6.06% to 5.51% (p = 0.001). CPRs at wards were significantly reduced (0.52% to 0.34%, p = 0.012). The number of patients transferred to the ICU also decreased significantly (3.63% to 3.49%, p = 0.035). Using multivariate analysis, the intensive period was associated with reducing AEs (p = 0.019). In conclusion, we constructed an E-NEWS system, updating the NEWS every hour automatically. Implementing the E-NEWS was associated with a reduction in AEs, especially CPRs at wards and transfers to ICU from ordinary wards.
Psychological and Physiological Features Associated with Swimming Performance
Authors: Clemente-Suárez Vicente Javier , Fuentes-García Juan Pedro , Fernandes Ricardo J. , Vilas-Boas João Paulo
Published: April 2021

Background: The aim of the present research was to study the psychological and physiological features associated with aerobic and anaerobic performance in trained swimmers. Methods: A correlation and stepwise regression analyses were conducted with the data obtained in a RESTQ-76 sport questionnaire, a heart rate variability test, and an anaerobic and aerobic swimming performance efforts of 20 swimmers. Results: Aerobic performance correlated, principally, with parameters related to parasympathetic modulation measured in the frequency and time domains of the heart rate variability (LF/HF r: −0.806, p < 0.001; NN50 r: 0.937, p < 0.001). Swimmers’ anaerobic performance correlated to psychological features (low stress r: 0.526, p: 0.025, and high fatigue r: −0.506, p: 0.032). Conclusion: Swimming performance presented different psychological and physiological features depending on the probe characteristic. Specifically, swimmers’ anaerobic performance was associated with psychological features (low stress and high fatigue perception) and aerobic performance with physiological features (high parasympathetic modulation). This information could help coaches to know the variables to control in their swimmers, depending on the probe in which they compete (anaerobic or aerobic).
On the Rocky Road to Independence: Big Five Personality Traits and Locus of Control in Polish Primary School Students during Transition into Early Adolescence
Authors: Filipiak Sara , Łubianka Beata
Published: April 2021

This article reports the results of a survey of 455 Polish primary school sixth-graders experiencing changes in the education system. The goal of the study was to identify the relationships between the Big Five personality traits, measured with the picture-based personality survey for children (PBPS-C) and locus of control, determined using the locus of control questionnaire (LOCQ). The results lead to the conclusion that primary school students do not have an established locus of control of either success or failure. There are also no significant differences between boys and girls in the way they interpret the causes of situations and events that happen to them. Boys, compared to girls, scored significantly higher on traits related to seeking and enjoying the company of others. On the other hand, girls exhibited significantly higher levels of traits responsible for increased anxiety than boys. The personality traits that correlated the strongest with locus of control were Conscientiousness, Openness to Experience, and Agreeableness. A regression model showed that locus of control of success was significantly affected by two traits: Extraversion and Conscientiousness. Locus of control of failure was significantly predicted by Extraversion, Openness to Experience, Agreeableness (positively), and Neuroticism (negatively). Regression model with gender as a moderator of relationships between personality traits and locus of control turned out to be insignificant.