bluuebunny/crossref_metadata_2025 huggingface dataset:
Glutathione Metabolism and the Novel Role of Mitochondrial GSH in Retinal Degeneration
Authors: Sreekumar Parameswaran G. , Ferrington Deborah A. , Kannan Ram
Published: April 2021

Glutathione (GSH) is present ubiquitously, and its role as a crucial cellular antioxidant in tissues, including the retina, is well established. GSH’s antioxidant function arises from its ability to scavenge reactive oxygen species or to serve as an essential cofactor for GSH S-transferases and peroxidases. This review summarizes the general functions, retinal distribution, disorders linked to GSH deficiency, and the emerging role for mitochondrial GSH (mGSH) in retinal function. Though synthesized only in the cytosol, the presence of GSH in multiple cell organelles suggests the requirement for its active transport across organellar membranes. The localization and distribution of 2-oxoglutarate carrier (OGC) and dicarboxylate carrier (DIC), two recently characterized mitochondrial carrier proteins in RPE and retina, show that these transporters are highly expressed in human retinal pigment epithelium (RPE) cells and retinal layers, and their expression increases with RPE polarity in cultured cells. Depletion of mGSH levels via inhibition of the two transporters resulted in reduced mitochondrial bioenergetic parameters (basal respiration, ATP production, maximal respiration, and spare respiratory capacity) and increased RPE cell death. These results begin to reveal a critical role for mGSH in maintaining RPE bioenergetics and cell health. Thus, augmentation of mGSH pool under GSH-deficient conditions may be a valuable tool in treating retinal disorders, such as age-related macular degeneration and optic neuropathies, whose pathologies have been associated with mitochondrial dysfunction.
Effects of Drying Methods on Antioxidant, Anti-Inflammatory, and Anticancer Potentials of Phenolic Acids in Lovage Elicited by Jasmonic Acid and Yeast Extract
Authors: Złotek Urszula , Lewicki Sławomir , Markiewicz Anna , Szymanowska Urszula , Jakubczyk Anna
Published: April 2021

The study presents the effect of drying methods (traditional, convection, microwave, and freeze-drying) on the content and bioactivity (determined as antioxidative, anti-inflammatory, and antiproliferative potential) of potentially bioavailable fractions of phenolic acids contained in lovage elicited with jasmonic acid (JA) and yeast extract (YE) and in untreated control leaves. The highest amount of syringic acid was recorded in the convectionally dried lovage samples, while ethanolic extracts from lyophilized lovage had the highest content of protocatechuic and caffeic acids. The drying method significantly influenced the tested properties only in some cases. The traditional drying resulted in lower antioxidant potential, while convectional drying caused a reduction of the lipoxygenase inhibition ability of the samples after simulated digestion. Samples containing the control and elicited lovage leaves dried with convectional and traditional methods exhibited the highest cytotoxicity against a prostate cancer epithelial cell line.
Thermal Behavior Improvement of Fortified Commercial Avocado (Persea americana Mill.) Oil with Maqui (Aristotelia chilensis) Leaf Extracts
Authors: Flores Marcos , Reyes-García Luis , Ortiz-Viedma Jaime , Romero Nalda , Vilcanqui Yesica , Rogel Cristian , Echeverría Javier , Forero-Doria Oscar
Published: April 2021

Avocado oil is considered a highly prized food due to its nutritional contribution. On the other hand, Aristotelia chilensis (Molina) Stuntz (Elaeocarpaceae), common name “maqui”, is an endemic fruit in Chile, well known for its exceptional antioxidant properties. In general, maqui by-products such as leaves are considered as waste. Thus, maqui leaves extracts were used to improve the stability of vegetable oils, particularly avocado oil. Hence, avocado oil was fortified with two extracts (ethyl ether and methanol) obtained of maqui leaves and exposed to 120 °C for 386 h in an oven. The results showed a high content of monounsaturated fatty acids (69.46%, mainly oleic acid), followed by polyunsaturated fatty acids (16.41%, mainly linoleic acid) and finally saturated fatty acids (14.13%). The concentration of the total phenolic compounds in the pure oil, ethyl ether and methanol maqui leaves extracts were 45.8, 83.7, and 4100.9 ppm, respectively. In addition, the antioxidant activity was 5091.6 and 19,452.5 µmol Trolox eq/g for the ethyl ether and methanol extracts, respectively. The secondary degradation compounds showed significant differences between the fortified and non-fortified samples after 144 h and the TG/DTG analysis showed a significant increment of 7 °C in the degradation temperature (Tonset) of avocado oil fortified with the methanol extract when compared to the non-fortified oil and fortified oil with ethyl ether extract. After heating for 336 h, fortified oil with methanol extract reached the limit percentages of polar compounds, while pure oil reached it in a shorter time, i.e., 240 h. Based on the results, avocado oil can be protected with natural additives such as extracts obtained from maqui leaves, leading to an increase in its thermo-oxidative stability.
Development of Highly Sensitive Temperature Microsensors for Localized Measurements
Authors: Sousa Paulo J. , Pinto Vânia C. , Magalhães Vitor H. , Rodrigues Raquel O. , Sousa Patrícia C. , Minas Graça
Published: April 2021

This paper presents the design, fabrication and characterization of temperature microsensors based on Resistance Temperature Detectors (RTDs) with a meander-shaped geometry. Numerical simulations were performed for studying the sensitivity of the RTDs according to their windings numbers as well as for optimizing their layout. These RTDs were fabricated using well-established microfabrication and photolithographic techniques. The fabricated sensors feature high sensitivity (0.3542 mV/°C), linearity and reproducibility in a temperature range of 35 to 45 °C. Additionally, each sensor has a small size with a strong potential for their integration in microfluidic devices, as organ-on-a-chip, allowing the possibility for in-situ monitoring the physiochemical properties of the cellular microenvironment.
Climate Transition from Warm-Dry to Warm-Wet in Eastern Northwest China
Authors: Yang Jinhu , Zhang Qiang , Lu Guoyang , Liu Xiaoyun , Wang Youheng , Wang Dawei , Liu Weiping , Yue Ping , Zhu Biao , Duan Xinyu
Published: April 2021

During the second half of the 20th century, eastern Northwest China experienced a warming and drying climate change. To determine whether this trend has continued or changed during the present century, this study systematically analyzes the characteristics of warming and dry–wet changes in eastern Northwest China based on the latest observational data and World Climate Research Programme (WCRP) Coupled Model Intercomparison Project Phase 6 (CMIP6) collection data. The results show that eastern Northwest China has warmed continuously during the past 60 years with a sudden temperature change occurring in the late 1990s. However, the temperature in the 2000s decreased slowly, and that in the 2010s showed a warming trend. The amount of precipitation began to increase in the late 1990s, which indicates a contemporary climate transition from warm-dry to warm-wet in eastern Northwest China. The contribution of precipitation to humidity is significantly more than that of temperature. Long-term and interannual variations dominate the temperature change, with the contribution of the former much stronger than that of the latter. However, interannual variation dominates the precipitation change. The warming accelerates from period to period, and the temperature spatial consistently increased during the three most recent climatic periods. The precipitation decreased from 1961–1990 to 1981–2010, whereas its spatial consistency increased from 1981–2010 to 1991–2019. The significant warming and humidification which began in the late 1990s and is expected to continue until the end of the 21st century in the medium emission scenario. However, the current sub-humid climate will not easily be changed. The warming could cause a climate transition from warm temperate to subtropical by 2040. The dry-to-wet climate transition in eastern Northwest China could be related to a synergistic enhancement of the East Asian summer monsoon and the westerly circulation. This research provides a scientific decision-making basis for implementing western development strategies, ecological protection, and high-quality development of the Yellow River Basin Area as well as that for ecological construction planning and water resource management of eastern Northwest China.
Dopamine Transporter Genetic Reduction Induces Morpho-Functional Changes in the Enteric Nervous System
Authors: Cerantola Silvia , Caputi Valentina , Contarini Gabriella , Mereu Maddalena , Bertazzo Antonella , Bosi Annalisa , Banfi Davide , Mantini Dante , Giaroni Cristina , Giron Maria Cecilia
Published: April 2021

Antidopaminergic gastrointestinal prokinetics are indeed commonly used to treat gastrointestinal motility disorders, although the precise role of dopaminergic transmission in the gut is still unclear. Since dopamine transporter (DAT) is involved in several brain disorders by modulating extracellular dopamine in the central nervous system, this study evaluated the impact of DAT genetic reduction on the morpho-functional integrity of mouse small intestine enteric nervous system (ENS). In DAT heterozygous (DAT+/−) and wild-type (DAT+/+) mice (14 ± 2 weeks) alterations in small intestinal contractility were evaluated by isometrical assessment of neuromuscular responses to receptor and non-receptor-mediated stimuli. Changes in ENS integrity were studied by real-time PCR and confocal immunofluorescence microscopy in longitudinal muscle-myenteric plexus whole-mount preparations (). DAT genetic reduction resulted in a significant increase in dopamine-mediated effects, primarily via D1 receptor activation, as well as in reduced cholinergic response, sustained by tachykininergic and glutamatergic neurotransmission via NMDA receptors. These functional anomalies were associated to architectural changes in the neurochemical coding and S100β immunoreactivity in small intestine myenteric plexus. Our study provides evidence that genetic-driven DAT defective activity determines anomalies in ENS architecture and neurochemical coding together with ileal dysmotility, highlighting the involvement of dopaminergic system in gut disorders, often associated to neurological conditions.
SIRT1-Dependent Upregulation of BDNF in Human Microglia Challenged with Aβ: An Early but Transient Response Rescued by Melatonin
Authors: Caruso Grazia Ilaria , Spampinato Simona Federica , Costantino Giuseppe , Merlo Sara , Sortino Maria Angela
Published: April 2021

Microglia represent a first-line defense in the brain. However, in pathological conditions such as Alzheimer’s disease (AD), a pro-inflammatory switch may occur, leading to loss of protective functions. Using the human microglial cell line HMC3, we showed that exposure to low concentrations of β-amyloid peptide 1-42 (Aβ42; 0.2 μM) initially (6 h) upregulated anti-inflammatory markers interleukin (IL)-4, IL-13, and brain-derived neurotrophic factor (BDNF). BDNF increase was prevented by selective inhibition of SIRT1 with EX527 (2 μM). Accordingly, these early effects were accompanied by a significant Aβ42-induced increase of SIRT1 expression, nuclear localization, and activity. SIRT1 modulation involved adenosine monophosphate-regulated kinase (AMPK), which was promptly (30 min) phosphorylated by Aβ42, while the AMPK inhibitor BML-275 (2 μM) attenuated Aβ42-induced SIRT1 increase. Initially observed microglial responses appeared transient, as microglial features changed when exposure to Aβ42 was prolonged (0.2 μM for 72 h). While SIRT1 and BDNF levels were reduced, the expression of inflammatory markers IL-1β and tumor necrosis factor (TNF)-α increased. This coincided with a rise in NF-kB nuclear localization. The effects of melatonin (1 μM) on prolonged microglial exposure to Aβ42 were analyzed for their protective potential. Melatonin was able to prolong SIRT1 and BDNF upregulation, as well as to prevent NF-kB nuclear translocation and acetylation. These effects were sensitive to the melatonin receptor antagonist, luzindole (25 μM). In conclusion, our data define an early microglial defensive response to Aβ42, featuring SIRT1-mediated BDNF upregulation that can be exogenously modulated by melatonin, thus identifying an important target for neuroprotection.
HIF-α Prolyl Hydroxylase Inhibitors and Their Implications for Biomedicine: A Comprehensive Review
Authors: Hirota Kiichi
Published: April 2021

Oxygen is essential for the maintenance of the body. Living organisms have evolved systems to secure an oxygen environment to be proper. Hypoxia-inducible factor (HIF) plays an essential role in this process; it is a transcription factor that mediates erythropoietin (EPO) induction at the transcriptional level under hypoxic environment. After successful cDNA cloning in 1995, a line of studies were conducted for elucidating the molecular mechanism of HIF activation in response to hypoxia. In 2001, cDNA cloning of dioxygenases acting on prolines and asparagine residues, which play essential roles in this process, was reported. HIF-prolyl hydroxylases (PHs) are molecules that constitute the core molecular mechanism of detecting a decrease in the partial pressure of oxygen, or hypoxia, in the cells; they can be called oxygen sensors. In this review, I discuss the process of molecular cloning of HIF and HIF-PH, which explains hypoxia-induced EPO expression; the development of HIF-PH inhibitors that artificially or exogenously activate HIF by inhibiting HIF-PH; and the significance and implications of medical intervention using HIF-PH inhibitors.
Stimulation of Different Sectors of the Human Dorsal Premotor Cortex Induces a Shift from Reactive to Predictive Action Strategies and Changes in Motor Inhibition: A Dense Transcranial Magnetic Stimulation (TMS) Mapping Study
Authors: Cattaneo Luigi , Parmigiani Sara
Published: April 2021

Delayed motor tasks require timely interaction between immobility and action. The neural substrates of these processes probably reside in the premotor and motor circuits; however, fine-grained anatomical/functional information is still lacking. Participants performed a delayed simple reaction task, structured as a ready-set-go sequence, with a fixed, predictable, SET-period. Responses were given with lip movements. During the SET-period, we performed a systematic dense-mapping of the bilateral dorsal premotor region (dPM) by means of single transcranial magnetic stimulation (TMS) pulses on an 18-spot mapping grid, interleaved with sham TMS which served as a baseline. Reaction times (RTs) in TMS trials over each grid spot were compared to RTs in sham trials to build a statistical parametric z-map. The results reveal a rostro-caudal functional gradient in the dPM. TMS of the rostral dPM induced a shift from reactive towards predictive response strategies. TMS of the caudal dPM interfered with the SET-period duration. By means of dense TMS mapping, we have drawn a putative functional map of the role of the dPM during the SET-period. A higher-order rostral component is involved in setting action strategies and a caudal, lower-order, part is probably involved in the inhibitory control of motor output.
Distinction of Physiologic and Epileptic Ripples: An Electrical Stimulation Study
Authors: Schönberger Jan , Knopf Anja , Klotz Kerstin Alexandra , Dümpelmann Matthias , Schulze-Bonhage Andreas , Jacobs Julia
Published: April 2021

Ripple oscillations (80–250 Hz) are a promising biomarker of epileptic activity, but are also involved in memory consolidation, which impairs their value as a diagnostic tool. Distinguishing physiologic from epileptic ripples has been particularly challenging because usually, invasive recordings are only performed in patients with refractory epilepsy. Here, we identified ‘healthy’ brain areas based on electrical stimulation and hypothesized that these regions specifically generate ‘pure’ ripples not coupled to spikes. Intracranial electroencephalography (EEG) recorded with subdural grid electrodes was retrospectively analyzed in 19 patients with drug-resistant focal epilepsy. Interictal spikes and ripples were automatically detected in slow-wave sleep using the publicly available Delphos software. We found that rates of spikes, ripples and ripples coupled to spikes (‘spike–ripples’) were higher inside the seizure-onset zone (p < 0.001). A comparison of receiver operating characteristic curves revealed that spike–ripples slightly delineated the seizure-onset zone channels, but did this significantly better than spikes (p < 0.001). Ripples were more frequent in the eloquent neocortex than in the remaining non-seizure onset zone areas (p < 0.001). This was due to the higher rates of ‘pure’ ripples (p < 0.001; median rates 3.3/min vs. 1.4/min), whereas spike–ripple rates were not significantly different (p = 0.87). ‘Pure’ ripples identified ‘healthy’ channels significantly better than chance (p < 0.001). Our findings suggest that, in contrast to epileptic spike–ripples, ‘pure’ ripples are mainly physiological. They may be considered, in addition to electrical stimulation, to delineate eloquent cortex in pre-surgical patients. Since we applied open source software for detection, our approach may be generally suited to tackle a variety of research questions in epilepsy and cognitive science.
Experimental Behavior of Steel-Concrete Composite Girders with UHPC-Grout Strip Shear Connection
Authors: He Zhi-Qi , Ou Changxue , Tian Fei , Liu Zhao
Published: April 2021

This paper develops a new type of shear connection for steel-concrete composite bridges using Ultra-High Performance Concrete (UHPC) as the connection grout. The UHPC-grout strip shear connection is fabricated by preforming a roughened slot in the concrete deck slab, welding an embossed steel rib longitudinally to the upper flange of the steel girder, and casting the strip void between the slot and the steel rib with UHPC grout. The structural performance of the new connection was validated by two sets of experimental tests, including push-out testing of shear connectors and static and fatigue testing of composite beams. The results of push-out testing indicate that the UHPC-grout strip shear connection exhibits a significant improvement of ductility, ultimate capacity, and fatigue performance. The interface shear strength of the UHPC-grout strip connection is beyond 15 MPa, which is about three times that of the strip connection using traditional cementitious grouts. The ultimate capacity of the connection is dominated by the interface failure between the embossed steel and the UHPC grout. The results of composite-beam testing indicate that full composite action is developed between the precast decks and the steel beams, and the composite action remained intact after testing for two million load cycles. Finally, the trail design of a prototype bridge shows that this new connection has the potential to meet the requirements for horizontal shear.
Polyamide 12/Multiwalled Carbon Nanotube and Carbon Black Nanocomposites Manufactured by 3D Printing Fused Filament Fabrication: A Comparison of the Electrical, Thermoelectric, and Mechanical Properties
Authors: Vidakis Nectarios , Petousis Markos , Tzounis Lazaros , Velidakis Emmanuel , Mountakis Nikolaos , Grammatikos Sotirios A.
Published: April 2021

In this study, nanocomposites with polyamide 12 (PA12) as the polymer matrix and multiwalled carbon nanotubes (MWCNTs) and carbon black (CB) at different loadings (2.5, 5.0, and 10.0 wt.%) as fillers, were produced in 3D printing filament form by melt mixing extrusion process. The filament was then used to build specimens with the fused filament fabrication (FFF) three-dimensional (3D) printing process. The aim was to produce by FFF 3D printing, electrically conductive and thermoelectric functional specimens with enhanced mechanical properties. All nanocomposites’ samples were electrically conductive at filler loadings above the electrical percolation threshold. The highest thermoelectric performance was obtained for the PA12/CNT nanocomposite at 10.0 wt.%. The static tensile and flexural mechanical properties, as well as the Charpy’s impact and Vickers microhardness, were determined. The highest improvement in mechanical properties was observed for the PA12/CNT nanocomposites at 5.0 wt.% filler loading. The fracture mechanisms were identified by fractographic analyses of scanning electron microscopy (SEM) images acquired from fractured surfaces of tensile tested specimens. The nanocomposites produced could find a variety of applications such as; 3D-printed organic thermoelectric materials for plausible large-scale thermal energy harvesting applications, resistors for flexible circuitry, and piezoresistive sensors for strain sensing.
The PARP Enzyme Family and the Hallmarks of Cancer Part 2: Hallmarks Related to Cancer Host Interactions
Authors: Demény Máté A. , Virág László
Published: April 2021

Poly (ADP-ribose) polymerases (PARPs) modify target proteins with a single ADP-ribose unit or with a poly (ADP-ribose) (PAR) polymer. PARP inhibitors (PARPis) recently became clinically available for the treatment of BRCA1/2 deficient tumors via the synthetic lethality paradigm. This personalized treatment primarily targets DNA damage-responsive PARPs (PARP1–3). However, the biological roles of PARP family member enzymes are broad; therefore, the effects of PARPis should be viewed in a much wider context, which includes complex effects on all known hallmarks of cancer. In the companion paper (part 1) to this review, we presented the fundamental roles of PARPs in intrinsic cancer cell hallmarks, such as uncontrolled proliferation, evasion of growth suppressors, cell death resistance, genome instability, replicative immortality, and reprogrammed metabolism. In the second part of this review, we present evidence linking PARPs to cancer-associated inflammation, anti-cancer immune response, invasion, and metastasis. A comprehensive overview of the roles of PARPs can facilitate the identification of novel cancer treatment opportunities and barriers limiting the efficacy of PARPi compounds.
Colibactin-Producing Escherichia coli Induce the Formation of Invasive Carcinomas in a Chronic Inflammation-Associated Mouse Model
Authors: Salesse Laurène , Lucas Cécily , Hoang My Hanh Thi , Sauvanet Pierre , Rezard Alexandra , Rosenstiel Philip , Damon-Soubeyrand Christelle , Barnich Nicolas , Godfraind Catherine , Dalmasso Guillaume , Nguyen Hang Thi Thu
Published: April 2021

Background: Escherichia coli producing the genotoxin colibactin (CoPEC or colibactin-producing E. coli) abnormally colonize the colonic mucosa of colorectal cancer (CRC) patients. We previously showed that deficiency of autophagy in intestinal epithelial cells (IECs) enhances CoPEC-induced colorectal carcinogenesis in ApcMin/+ mice. Here, we tested if CoPEC trigger tumorigenesis in a mouse model lacking genetic susceptibility or the use of carcinogen. Methods: Mice with autophagy deficiency in IECs (Atg16l1∆IEC) or wild-type mice (Atg16l1flox/flox) were infected with the CoPEC 11G5 strain or the mutant 11G5∆clbQ incapable of producing colibactin and subjected to 12 cycles of DSS treatment to induce chronic colitis. Mouse colons were used for histological assessment, immunohistochemical and immunoblot analyses for DNA damage marker. Results: 11G5 or 11G5∆clbQ infection increased clinical and histological inflammation scores, and these were further enhanced by IEC-specific autophagy deficiency. 11G5 infection, but not 11G5∆clbQ infection, triggered the formation of invasive carcinomas, and this was further increased by autophagy deficiency. The increase in invasive carcinomas was correlated with enhanced DNA damage and independent of inflammation. Conclusions: CoPEC induce colorectal carcinogenesis in a CRC mouse model lacking genetic susceptibility and carcinogen. This work highlights the role of (i) CoPEC as a driver of CRC development, and (ii) autophagy in inhibiting the carcinogenic properties of CoPEC.
High-Grade Gliomas in Children—A Multi-Institutional Polish Study
Authors: Napieralska Aleksandra , Krzywon Aleksandra , Mizia-Malarz Agnieszka , Sosna-Zielińska Joanna , Pawłowska Ewa , Krawczyk Małgorzata A. , Konat-Bąska Katarzyna , Kaczorowska Aneta , Dąbrowska Anna , Harat Maciej
Published: April 2021

Due to the rarity of high-grade gliomas (HGG) in children, data on this topic are scarce. The study aimed to investigate the long-term results of treatment of children with HGG and to identify factors related to better survival. We performed a retrospective analysis of patients treated for HGG who had the main tumor located outside the brainstem. The evaluation of factors that correlated with better survival was performed with the Cox proportional-hazard model. Survival was estimated with the Kaplan–Meier method. The study group consisted of 82 consecutive patients. All of them underwent surgery as primary treatment. Chemotherapy was applied in 93% of children with one third treated with temozolomide. After or during the systemic treatment, 79% of them received radiotherapy with a median dose of 54 Gy. Median follow-up was 122 months, and during that time, 59 patients died. One-, 2-, 5-, and 10-year overall survival was 78%, 48%, 30% and 17%, respectively. Patients with radical (R0) resection and temozolomide-based chemotherapy had better overall survival. Progression-free survival was better in patients after R0 resection and radical radiotherapy. The best outcome in HGG patients was observed in patients after R0 resection with immediate postoperative temozolomide-based chemotherapy and radical radiotherapy.
Overview of Subcutaneous Metastatic Melanoma
Authors: Mozzillo Nicola
Published: April 2021

Melanoma is among the most common primary solid tumors with cutaneous and subcutaneous metastasis [...]
Application of Response Surface Methodology for Preparation of ZnAC2/CAC Adsorbents for Hydrogen Sulfide (H2S) Capture
Authors: Zulkefli Nurul Noramelya , Masdar Mohd Shahbudin , Wan Isahak Wan Nor Roslam , Abu Bakar Siti Nur Hatika , Abu Hasan Hassimi , Mohd Sofian Nabilah
Published: April 2021

Hydrogen sulfide (H2S) should be removed in the early stage of biogas purification as it may affect biogas production and cause environmental and catalyst toxicity. The adsorption of H2S gas by using activated carbon as a catalyst has been explored as a possible technology to remove H2S in the biogas industry. In this study, we investigated the optimal catalytic preparation conditions of the H2S adsorbent by using the RSM methodology and the Box–Behnken experimental design. The H2S catalyst was synthesized by impregnating commercial activated carbon (CAC) with zinc acetate (ZnAc2) with the factors and level for the Box–Behnken Design (BBD): molarity of 0.2–1.0 M ZnAc2 solution, soaked temperature of 30–100 °C, and soaked time of 30–180 min. Two responses including the H2S adsorption capacity and the BET surface area were assessed using two-factor interaction (2FI) models. The interactions were examined by using the analysis of variance (ANOVA). Hence, the optimum point of molarity was 0.22 M ZnAc2 solution, the soaked period was 48.82 min, and the soaked temperature was 95.08 °C obtained from the optimum point with the highest H2S adsorption capacity (2.37 mg H2S/g) and the optimum BET surface area (620.55 m2/g). Additionally, the comparison of the optimized and the non-optimized catalytic adsorbents showed an enhancement in the H2S adsorption capacity of up to 33%.
Indium-Catalyzed Cycloisomerization of 1,6-Cyclohexenylalkynes
Authors: Davenel Vincent , Puteaux Chloé , Nisole Christian , Fontaine-Vive Fabien , Fourquez Jean-Marie , Michelet Véronique
Published: April 2021

Efficient four- and five-step routes to access functionalized bicyclo[3.2.1]oct-2-ene and bicyclo[3.3.1]nonadiene via indium-mediated cycloisomerization of 1,6-enynes has been developed. This atom-economical catalytic process was optimized and relied on the efficiency of InCl3 leading to the preparation of functionalized bicyclic adducts in up to 99% isolated yield. The cyclization occurred on two different processes (5-exo versus 6-endo pathway) and were influenced by the substitution of the alkynyl moiety. The exo process was favored for non-substituted alkynes whereas the endo pathway was generally observed for substituted alkynes. Then, the presence of electron-withdrawing groups on the aryl substituted alkyne increased the ratio of the exo isomer. DFT calculations were performed on stability of intermediates and corroborated the intervention of InCl3.
Impact of COVID-19 Mitigation Policy in South Korea on the Reduction of Preterm or Low Birth Weight Birth Rate: A Single Center Experience
Authors: Kim Sae-Yun , Kim So-Young , Kil Kicheol , Lee Young
Published: April 2021

The government of South Korea implemented social distancing measures to control the coronavirus disease 2019 (COVID-19) outbreak. This study aimed to compare the composite preterm (PT) or low birth weight (LBW) birth rates during the COVID-19 pandemic period in South Korea to those during the prior decade, and to find out the associations of childbirth during the pandemic period with PT or LBW births. Over a ten-year period, this retrospective cohort study was performed in a single hospital in the Seoul metropolitan city. The COVID-19 period was defined as running from 22 March 2020, to 31 October 2020, and the pre-COVID-19 period as the sum of parallel periods from 2011 to 2019. Trends in composite birth rates were investigated, and logistic regression analysis was conducted to investigate independent factors associated with composite births. There were 246 and 2765 singleton deliveries during the COVID-19 period and the pre-COVID-19 period, respectively. The composite birth rate decreased from 16.5% to 9.8%. Childbirth during the pandemic was independently associated with a decreased composite birth rate (adjusted odds ratio, 0.563; 95% confidence interval, 0.355−0.844, p = 0.015). These findings suggested that the COVID-19 pandemic might provide an opportunity to find out preventive factors for PT or LBW births.
Evaluation of Correlations between Genetic Variants and High-Resolution Computed Tomography Patterns in Idiopathic Pulmonary Fibrosis
Authors: Baratella Elisa , Ruaro Barbara , Giudici Fabiola , Wade Barbara , Santagiuliana Mario , Salton Francesco , Confalonieri Paola , Simbolo Michele , Scarpa Aldo , Tollot Saverio , Marrocchio Cristina , Cova Maria Assunta , Confalonieri Marco
Published: April 2021

Background. Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease (ILD). This prospective observational study aimed at the evaluation of any correlation between genetic variants associated with IPF susceptibility and high-resolution computed tomography (HRCT) patterns. It also aimed at evidencing any differences in the HRTC pattern between the familial and sporadic form at diagnosis and after two years. Methods. A total of 65 IPF patients (mean age at diagnosis 65 ± 10) were enrolled after having given written informed consent. HRCT and genetic evaluations were performed. Results. A total of 19 familial (mean age 62 ± 15) and 46 sporadic (mean age 70 ± 9) IPF patients were enrolled. A statistically significant difference was evidenced in the HRTC pattern at diagnosis between the two groups. Sporadic IPF patients had a predominantly usual interstitial pneumonia (UIP) pattern compared with those patients with familial IPF (60.0% vs. 21.1%, respectively). Moreover, familial IPF patients had more alternative diagnoses than those with sporadic IPF (31.6% vs. 2.2%, respectively). Furthermore, there was a slight increase in the typical UIP pattern in the familial IPF group at two years from diagnosis. Conclusions. Genetic factors play a pivotal role in the risk of developing IPF. However, further studies are required to clarify how these genetic factors may guide clinical treatment decisions.
Intradermal Testing Doubles Identification of Allergy among 110 Immunotherapy-Responsive Patients with Eustachian Tube Dysfunction
Authors: Hurst David S. , Gordon Bruce R. , McDaniel Alan B. , Poe Dennis S.
Published: April 2021

The purpose of this study was to determine whether the sensitivity advantage of intradermal dilutional testing (IDT) is clinically relevant in patients with obstructive Eustachian tube dysfunction (ETD) or otitis media with effusion (OME). This retrospective, private-practice cohort study compared the sensitivity of skin prick tests (SPT) vs. IDT in 110 adults and children with suspected allergy and OME. Primary outcome measure was symptom resolution from allergy immunotherapy (AIT). IDT identified 57% more patients as being allergic, and 8.6 times more reactive allergens than would have been diagnosed using only SPT. Patients diagnosed by IDT had the same degree of symptom improvement from immunotherapy, independent of allergen sensitivity (66% by SPT vs. 63% by IDT; p = 0.69, not different). Low-sensitivity allergy tests, which may fail to identify allergy in over two thirds of children aged 3 to 15 as being atopic, or among 60% of patients with ETD, may explain why many physicians do not consider allergy as a treatable etiology for their patient’s OME/ETD. IDT offers superior sensitivity over SPT for detecting allergens clinically relevant to treating OME/ETD. These data strongly support increased utilization of intradermal testing and invite additional clinical outcome studies.
Intelligent Bone Age Assessment: An Automated System to Detect a Bone Growth Problem Using Convolutional Neural Networks with Attention Mechanism
Authors: Zulkifley Mohd Asyraf , Mohamed Nur Ayuni , Abdani Siti Raihanah , Kamari Nor Azwan Mohamed , Moubark Asraf Mohamed , Ibrahim Ahmad Asrul
Published: April 2021

Skeletal bone age assessment using X-ray images is a standard clinical procedure to detect any anomaly in bone growth among kids and babies. The assessed bone age indicates the actual level of growth, whereby a large discrepancy between the assessed and chronological age might point to a growth disorder. Hence, skeletal bone age assessment is used to screen the possibility of growth abnormalities, genetic problems, and endocrine disorders. Usually, the manual screening is assessed through X-ray images of the non-dominant hand using the Greulich–Pyle (GP) or Tanner–Whitehouse (TW) approach. The GP uses a standard hand atlas, which will be the reference point to predict the bone age of a patient, while the TW uses a scoring mechanism to assess the bone age using several regions of interest information. However, both approaches are heavily dependent on individual domain knowledge and expertise, which is prone to high bias in inter and intra-observer results. Hence, an automated bone age assessment system, which is referred to as Attention-Xception Network (AXNet) is proposed to automatically predict the bone age accurately. The proposed AXNet consists of two parts, which are image normalization and bone age regression modules. The image normalization module will transform each X-ray image into a standardized form so that the regressor network can be trained using better input images. This module will first extract the hand region from the background, which is then rotated to an upright position using the angle calculated from the four key-points of interest. Then, the masked and rotated hand image will be aligned such that it will be positioned in the middle of the image. Both of the masked and rotated images will be obtained through existing state-of-the-art deep learning methods. The last module will then predict the bone age through the Attention-Xception network that incorporates multiple layers of spatial-attention mechanism to emphasize the important features for more accurate bone age prediction. From the experimental results, the proposed AXNet achieves the lowest mean absolute error and mean squared error of 7.699 months and 108.869 months2, respectively. Therefore, the proposed AXNet has demonstrated its potential for practical clinical use with an error of less than one year to assist the experts or radiologists in evaluating the bone age objectively.
Optimizing Expected Shortfall under an ℓ1 Constraint—An Analytic Approach
Authors: Papp Gábor , Kondor Imre , Caccioli Fabio
Published: April 2021

Expected Shortfall (ES), the average loss above a high quantile, is the current financial regulatory market risk measure. Its estimation and optimization are highly unstable against sample fluctuations and become impossible above a critical ratio r=N/T, where N is the number of different assets in the portfolio, and T is the length of the available time series. The critical ratio depends on the confidence level α, which means we have a line of critical points on the α−r plane. The large fluctuations in the estimation of ES can be attenuated by the application of regularizers. In this paper, we calculate ES analytically under an ℓ1 regularizer by the method of replicas borrowed from the statistical physics of random systems. The ban on short selling, i.e., a constraint rendering all the portfolio weights non-negative, is a special case of an asymmetric ℓ1 regularizer. Results are presented for the out-of-sample and the in-sample estimator of the regularized ES, the estimation error, the distribution of the optimal portfolio weights, and the density of the assets eliminated from the portfolio by the regularizer. It is shown that the no-short constraint acts as a high volatility cutoff, in the sense that it sets the weights of the high volatility elements to zero with higher probability than those of the low volatility items. This cutoff renormalizes the aspect ratio r=N/T, thereby extending the range of the feasibility of optimization. We find that there is a nontrivial mapping between the regularized and unregularized problems, corresponding to a renormalization of the order parameters.
Hybrid Memristor–CMOS Implementation of Combinational Logic Based on X-MRL
Authors: Ali Khaled Alhaj , Rizk Mostafa , Baghdadi Amer , Diguet Jean-Philippe , Jomaah Jalal
Published: April 2021

A great deal of effort has recently been devoted to extending the usage of memristor technology from memory to computing. Memristor-based logic design is an emerging concept that targets efficient computing systems. Several logic families have evolved, each with different attributes. Memristor Ratioed Logic (MRL) has been recently introduced as a hybrid memristor–CMOS logic family. MRL requires an efficient design strategy that takes into consideration the implementation phase. This paper presents a novel MRL-based crossbar design: X-MRL. The proposed structure combines the density and scalability attributes of memristive crossbar arrays and the opportunity of their implementation at the top of CMOS layer. The evaluation of the proposed approach is performed through the design of an X-MRL-based full adder. The design is presented with its layout and corresponding simulation results using the Cadence Virtuoso toolset and CMOS 65nm process. The comparison with a pure CMOS implementation is promising in terms of the area, as our approach exhibits a 44.79% area reduction. Moreover, the combined Energy.Delay metric demonstrates a significant improvement (between ×5.7 and ×31) with respect to the available literature.
Empirical Validation of a Biogas Plant Simulation Model and Analysis of Biogas Upgrading Potentials
Authors: Zepter Jan Martin , Engelhardt Jan , Gabderakhmanova Tatiana , Marinelli Mattia
Published: April 2021

Biogas plants may support the transformation towards renewable-based and integrated energy systems by providing dispatchable co-generation as well as opportunities for biogas upgrading or power-to-X conversion. In this paper, a simulation model that comprises the main dynamics of the internal processes of a biogas plant is developed. Based on first-order kinetics of the anaerobic digestion process, the biogas production of an input feeding schedule of raw material can be estimated. The output of the plant in terms of electrical and thermal energy is validated against empirical data from a 3-MW biogas plant on the Danish island of Bornholm. The results show that the model provides an accurate representation of the processes within a biogas plant. The paper further provides insights on the functioning of the biogas plant on Bornholm as well as discusses upgrading potentials of biogas to biomethane at the plant from an energy perspective.
Economics of Battery Use in Agriculture: Economic Viability of Renewable Energy Complemented with Batteries in Agriculture
Authors: Fuchs Clemens , Poehls Axel , Skau Katharina , Kasten Joachim
Published: April 2021

The production of renewable energy fluctuates in terms of sun and wind and must be supplemented by storage in the system. On an individual basis, i.e., for centralized electricity production and predominantly self-consumption, the use of batteries is considered here. Possible future development scenarios were simulated based on current price relationships (status quo). In the status quo, a selling price for PV electricity of 13 Euro cents (ct)ct/kWh was assumed with a production cost of 11 ct/kWh. The selling price of wind power is 5 ct/kWh with a production cost of 3 ct/kWh. The cost of storing electricity in a battery increases the price by 33 ct/kWh. A price of 20 ct/kWh is assumed for electricity purchases by companies. In the status quo, the use of batteries is not economical given the assumed price relationships. Changing the framework conditions, such as those of the legislature in Germany with the nuclear power phase-out and in the EU with the coal exit and decarbonization, will lead to increased availability of (fluctuating) renewable electricity, especially during the day. The purchase of electricity at other times, when the supply is scarce, can lead to increased electricity prices, especially at night. Together with falling costs for storage, the use of batteries for centralized power generators could be very interesting in the future. The method used in this study is nonlinear optimization of the target function costs of electricity supply in the developed simulation model. The results can also be transferred to other countries, as the assumed trends apply worldwide.
Effect of Pole Shoe Design on Inclination Angle of Different Magnetic Fields in Permanent Magnet Machines
Authors: Sjölund Jonathan , Eriksson Sandra
Published: April 2021

Electromagnetic modelling of electrical machines through finite element analysis is an important design tool for detailed studies of high resolution. Through the usage of finite element analysis, one can study the electromagnetic fields for information that is often difficult to acquire in an experimental test bench. The requirement for accurate result is that the magnetic circuit is modelled in a correct way, which may be more difficult to maintain for rare earth free permanent magnets with an operating range that is more likely to be close to non-linear regions for the relation between magnetic flux density and magnetic field strength. In this paper, the inclination angles of the magnetic flux density, magnetic field strength and magnetization are studied and means to reduce the inclination angles are investigated. Both rotating and linear machines are investigated in this paper, with different current densities induced in the stator windings. By proper design of the pole shoes, one can reduce the inclination angles of the fields in the permanent magnet. By controlling the inclination angles, one can both enhance the performance of the magnetic circuit and increase the accuracy of simpler models for permanent magnet modelling.
Endocrine Responses to Sport-Related Brain Injury in Female Athletes: A Narrative Review and a Call for Action
Authors: Duffy Donna , Elliott-Sale Kirsty J. , Gardner Hanna , Goldenstein Samantha , Wideman Laurie
Published: April 2021

Sport-related brain injury (SRBI) occurs when a blow to the head causes the brain to move back and forth in the skull, and can lead to neuroendocrine dysfunction. Research has shown that males and females experience and recover from SRBI differently, yet most of what is known regarding diagnosis, treatment, and recovery of SRBI is based on male normative data even though females meet or exceed incidence numbers of SRBIs compared to those of males. Females also have been known to have worse outcomes and a greater number of symptoms following SRBI than males. Research is limited as to why females have worse outcomes, but sex hormones have been suggested as a potential reason. SRBI may cause a dysregulation of the hypothalamic–pituitary–gonadal (HPG) axis, which is responsible for regulating the sex hormones estrogen and progesterone. Initial research has shown that SRBI may suppress estrogen and progesterone, and the concentration of these sex hormones could be indicative of injury severity and recovery trajectory. This review discusses the sex-specific differences in SRBI and also the future direction of research that is needed in order to identify the repercussions of SRBIs for female athletes, which will eventually lead to better clinical treatment, sideline care, and recovery profiles.
Autumnal Beach Litter Identification by Mean of Using Ground-Based IR Thermography
Authors: Cagnazzo Cosimo , Potente Ettore , Regnauld Hervé , Rosato Sabino , Mastronuzzi Giuseppe
Published: April 2021

The progress of scientific research and technological innovation are contributing to an increase in the use of rapid systems for monitoring and identifying geo-environmental processes related to natural and/or anthropogenic activities. The aim of this study is identifying autumnal beach litter using ground-based IR thermography. Starting from quarterly autumn monitoring data of air temperature and sandy soil surface temperature, an empirical equation between the two environmental matrices (air and sandy soil) is obtained. This will allow the calculation of the sandy soil surface temperature knowing only the air temperature. Therefore, it will be possible to know in advance the thermal response of the sandy soil, thus creating a thermal blank of the beach. Using an IR thermal camera, it is possible for a quicker identification of thermal anomalies of the coastal area potentially connected to the presence of pollution due to the anthropogenic origin (particularly plastic material). The test area is located in the area of the Coastal Dunes Regional Natural Park of Ostuni–Fasano in Apulia (southern Italy).
Withdrawal Properties of Self-Tapping Screws in Japanese larch (Larix kaempferi (Lamb.) Carr.) Cross Laminated Timber
Authors: Xu Junhua , Zhang Shuangbao , Wu Guofang , Gong Yingchun , Ren Haiqing
Published: April 2021

With the increasing popularity of cross-laminated timber (CLT) constructions around the world, there have been attempts to produce CLT using local wood species in different countries, such as Japanese larch (Larix kaempferi (Lamb.) Carr.) in China. Thus, the need to investigate the connection performance also increases to support the design and construction of CLT buildings using local wood species. In this study, the withdrawal properties of three different types of self-tapping screws (STS), with a diameter of 6 mm, 8 mm, and 11 mm, were tested with Japanese larch CLT. The results revealed that the withdrawal strength of STS increased with increasing density and effective length, but decreased with an increasing diameter. With a density increment of 0.05 g/cm3, the withdrawal strength increased by an average of 9.4%. With an effective length increment of 24 mm, the withdrawal strength increased by an average of 1.4%. An empirical regression model was adopted to predict the withdrawal strength of Japanese larch CLT based on the results, which can be used for potential engineering design of CLT connections using STS.