bluuebunny/crossref_metadata_2025 huggingface dataset:
“Who Has the Power to Adapt?” Frameworks for Resilient Agriculture Must Contend With the Power Dynamics of Land Tenure
Authors: Calo Adam
Published: December 2020

This special issue aims to develop how Diversified Farming Systems (DFS) may contribute to adaptive capacity in order to confer resilience to agricultural systems. In this perspective article, I argue that a framework for DFS and adaptive capacity must adequately contend with the role of farmland tenure on the shape of food systems to be both internally coherent and socially redistributive. Yet, both DFS and adaptive capacity scholarship deemphasize or mischaracterize the role of farmland tenure in favor of ecosystem dynamics. In this paper, I bring together lessons from the agrarian change literature and established critiques of resilience thinking to demonstrate core problems with a framework aimed at linking DFS to adaptive capacity without adequately addressing the role of farmland tenure. Namely, applying resilience thinking as a framework to understand food systems change prioritizes concern over final “states” or processes of farming systems and may ignore who has the power to adapt or who derives benefits from adaptation. The critiques of resilience thinking inform that the result of this apolitical elision is (1) entrenchment of neoliberal logics that place responsibility to cultivate adaptation on individual farmers and (2) provisioning of legitimacy for land tenure systems that can most readily adopt DFS, without understanding how well these systems distribute public benefits. Resilience reformers call for ways to include more power aware analysis when applying resilience thinking to complex socio-technical systems. I suggest that centering the role of land tenure into the frameworks of DFS and adaptive capacity provides a lens to observe the power relations that mediate any benefits of agricultural diversification. Integrating analysis of the social and legal structures of the food system into the DFS for adaptive capacity formulation is a crucial step to transforming resilience thinking from an apolitical tool to transformative and power-aware applied science.
An Overview of SARS-CoV-2 and Animal Infection
Authors: Mahdy Mohamed A. A. , Younis Waleed , Ewaida Zamzam
Published: December 2020

A novel coronavirus has been reported as the causative pathogen of the Coronavirus disease 2019 (COVID-19) outbreak in Wuhan city, China in December 2019. Due to the rapid spread of the virus worldwide, it has been announced as a pandemic by the World Health Organization (WHO). Hospitalized patients in Wuhan were associated with the Huanan seafood wholesale market where live animals, such as poultry, bats, snakes, frogs, rabbits, marmots, and hedgehogs are sold in that market which suggests a possible zoonotic infection. It was suggested that bat is the natural host of SARS-CoV-2, but the intermediate host is still unclear. It is essential to identify the potential intermediate host to interrupt the transmission chain of the virus. Pangolin is a highly suspected candidate as an intermediate host for SARS-CoV-2. Recently, SARS-CoV-2 infection has been reported in cats, dogs, tigers, and lions. More recently SARS-CoV-2 infection affected minks severely and zoonotic transfer with a variant SARS-CoV-2 strain evidenced in Denmark, Netherlands, USA, and Spain suggesting animal-to-human and animal-to-animal transmission within mink farms. Furthermore, experimental studies documented the susceptibility of different animal species to SARS-CoV-2, such as mice, golden hamsters, cats, ferrets, non-human primates, and treeshrews. It is also essential to know the possibility of infection for other animal species. This short review aims to provide an overview on the relation between severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and animals.
Dynamic Seismic Response of Nonlinear Displacement Dependent Devices versus Testing Required by Codes: Experimental Case Studies
Authors: Di Cesare Antonio , Ponzo Felice Carlo , Lamarucciola Nicla , Nigro Domenico
Published: December 2020

Passive energy dissipation systems are one of the most resilient solutions to mitigate the seismic risk of structures. In case of strong motions, they can confine the eventual damages into easily replaceable anti-seismic devices. The performance characteristics of nonlinear displacement dependent devices (NLD) shall be defined by the force-displacement cyclic behavior, as well as the expected number of cycles related to both the duration of the earthquake and to the fundamental frequency of the structural systems. The aims of this paper are the comparison between the dynamic results of two different experimental campaigns performed on NLDs included in dissipative bracing systems and the assessment of the reliability of quasi-static testing procedures proposed by current seismic codes for type tests and factory production control tests. The number of cycles under the design earthquake of hysteretic dampers were experimentally evaluated through shaking table testing. Two experimental case studies of a two-story steel frame and of a three-story post-tensioned timber frame both with bracing systems including flexural steel dampers, hysteretic dampers (HDs), and U-shaped flexural plates (UFPs) respectively, were analyzed. Controlled-displacement tests of NLDs were performed considering quasi-static loading procedures specified by codes. Shaking table tests were carried out considering almost the same seismic sequence composed by a set of seven natural earthquakes at increasing peak ground acceleration (PGA) levels. More than one hundred inelastic cycles were experimentally recorded from dynamic tests before the failure of devices in both cases. In line with American standards testing requirements, the number of cycles at the design PGA level, estimated from shaking table tests and from non-linear dynamic analyses, shows a decreasing trend with the increase of ductility demand.
Energy Balance, Cost and Architectural Design Features of 24 Building Integrated Photovoltaic Projects Using a Modelling Approach
Authors: Gercek Cihan , Devetaković Mirjana , Krstić-Furundžić Aleksandra , Reinders Angèle
Published: December 2020

This paper presents the energy balance, architectural design features and cost aspects of 24 building integrated photovoltaic (BIPV) projects in three different contexts, namely BIPV in residential, office and historical buildings. These BIPV projects have been modelled and evaluated for different geographic locations because the European Energy Performance of Buildings Directive (2018/844/EU) has resulted in country-specific regulations and situations aimed towards the reduction in energy consumption, and hence the CO2 emissions of built environments. Moreover, the geographical variation of irradiation affects the performance of different BIPV projects on different locations. The results of our study show that the return of investment of BIPV projects across 12 countries took (on average) 13.3 years. Furthermore, the residential projects —as compared to non-residential buildings—were mostly energy plus buildings with an average self-sufficiency of 110% due to their low energy consumption. In conclusion, most BIPV projects resulted in realistic energy performances (on average: 761 kWh/kWp.year), low payback times (10 years for residential and office buildings), and modelled unique design features.
Targeted Nanoparticles for Fluorescence Imaging of Folate Receptor Positive Tumors
Authors: Marko Aimee J. , Borah Ballav M. , Siters Kevin E. , Missert Joseph R. , Gupta Anurag , Pera Paula , Isaac-Lam Meden F. , Pandey Ravindra K.
Published: December 2020

This report presents the synthesis and folate receptor target-specificity of amino-functionalized polyacrylamide nanoparticles (AFPAA NPs) for near-infrared (NIR) fluorescence imaging of cancer. For the synthesis of desired nano-constructs, the AFPAA NPs (hereafter referred to as NPs) were reacted with a NIR cyanine dye (CD) bearing carboxylic acid functionality by following our previously reported approach, and the resulting conjugate (NP-CD) on further reaction with folic acid (FA) resulted in a new nano-construct, FA-NP-CD, which demonstrated significantly higher uptake in folate receptor-positive breast cancer cells (KB+) and in folate receptor over-expressed tumors in vivo. The target-specificity of these nanoparticles was further confirmed by inhibition assay in folate receptor-positive (KB+) and -negative (HT-1080) cell lines. To show the advantages of polyacrylamide (PAA)-based NPs in folate receptor target-specificity, the CD used in preparing the FA-NP-CD construct was also reacted with folic acid alone and the synthetic conjugate (CD-FA) was also investigated for its target-specificity. Interestingly, in contrast to NPs (FA-NP-CD), the CD-FA conjugate did not show any significant in vitro or in vivo specificity toward folate receptors, showing the advantages of PAA-based nanotechnology in delivering the desired agent to tumor cells.
Sildenafil–Resorcinol Cocrystal: XRPD Structure and DFT Calculations
Authors: Barbas Rafael , Kumar Vineet , Vallcorba Oriol , Prohens Rafel , Frontera Antonio
Published: December 2020

Herein, the X-ray powder diffraction (XRPD) crystal structure of a new Sildenafil cocrystal is reported, where resorcinol has been used as the coformer. The crystal structure has been solved by means of direct space methods used in combination with density functional theory (DFT) calculations. In the structure, the Sildenafil and resorcinol molecules form cooperative hydrogen bond (HB) and π-stacking interactions that have been analyzed using DFT calculations, the molecular electrostatic potential (MEP) surface, and noncovalent interaction plot (NCI plot). The formation of O–H⋯N H-bonds between resorcinol and Sildenafil increases the dipole moment and enhances the antiparallel π-stacking interaction.
Elaborating Validation Scenarios Based on the Context Analysis and Combinatorial Method: Example of the Power-Efficiency Framework Innomterics
Authors: Ciancarini Paolo , Kruglov Artem , Sadovykh Andrey , Succi Giancarlo , Zuev Evgeniy
Published: December 2020

The preliminary task of a project consists of the definition of the scenarios that will guide further development work and validate the results. In this paper, we present an approach for the systematic generation of validation scenarios using a specifically developed taxonomy and combinatorial testing. We applied this approach to our research project for the development of the energy-efficiency evaluation framework named Innometrics. We described in detail all steps for taxonomy creation, generation of abstract validation scenarios, and identification of relevant industrial and academic case studies. We created the taxonomy of the target computer systems and then elaborated test cases using combinatorial testing. The classification criteria were the type of the system, its purpose, enabling hardware components and connectivity technologies, basic design patterns, programming language, and development lifecycle. The combinatorial testing results in 13 cases for one-way test coverage, which was considered enough to create a comprehensive test suite. We defined the case study for each particular scenario. These case studies represent the real industrial, educational, and open-source software development projects that will be used in further work on the Innometrics project.
Energy Policy Concerns, Objectives and Indicators: A Review towards a Framework for Effectiveness Assessment
Authors: Ortiz Dania , Leal Vítor
Published: December 2020

This work presents a review that aims to characterize the policy evaluation practices regarding the public policies on energy, with a focus on the metrics: concerns, objectives, and indicators. As key novelty, emphasis was put into finding attributes and metrics that can be used to assess effectiveness, not only efficacy or efficiency. The concerns and objectives were organized into four categories: Institutional, Environmental, Economic, and Social. For every category, detailed and condensed concerns were identified. It was attempted to find indicators for every condensed concern, which resulted in 15 core indicators.
Lipid and Protein Oxidation Marker Compounds in Horse Meat Determined by MIR Spectroscopy
Authors: Jáuregui-López Irati , Zulategi Fernando , Beriain María José , Sarriés María Victoria , Beruete Miguel , Insausti Kizkitza
Published: December 2020

This work broadens the study of lipid and protein oxidation marker compounds in foal meat, employing the technology of Attenuated Total Reflectance-Fourier Transform Mid-Infrared Spectroscopy (ATR-FT/MIR, shortened in the following as MIR). As a main objective, marker compounds from 23 foals were extracted and their absorbance spectra were measured to establish prediction models (calibration and validation) between them and classical quantification analysis of the compounds. Another objective was to ascertain whether a previous extraction of the marker compounds before executing their MIR analysis is preferable compared to direct MIR measurements on the raw meat samples. In this context, marker compound results (TBARS between 0.4387 and 2.1040, and carbonyls between 4.07 and 4.68) showed more consistent predictive models than the ones achieved using quantitative analysis of the spectra obtained from the raw meat. Lipid oxidation compounds predictive models obtained in this work offered an R2cv of 63.18% and protein oxidation R2cv obtained in this project showed a value of 54.24%. Thus, MIR technology arises as a promising tool to identify and quantify products derived from lipid and protein oxidation in fresh foal meat.
Opinions on Insect Consumption in Hungary
Authors: Szendrő Katalin , Tóth Katalin , Nagy Mónika Zita
Published: December 2020

The aim of the study was to assess knowledge and opinion in the Hungarian population about the consumption of insect-based food. The questionnaire was filled in by 414 respondents. Their knowledge of edible insect consumption in different countries was average (score 4) or above on a scale of 1 (totally disagree) to 7 (totally agree). Their willingness to consume insect-based food was low, usually below average. Significantly higher scores were attained by men than women, by respondents with a university degree than those who graduated from secondary school, and the highest scores were attained by people of 30–39 age group. However, the effects of residence (town or village) and income were not significant. As Hungarians are not traditional insect consumers, there is a significant emotional response of disgust regarding food made from insects and, as insect food is not commercially available, no significant increase in insect consumption is expected in the near future.
Effect of Ohmic Heating on Sensory, Physicochemical, and Microbiological Properties of “Aguamiel” of Agave salmiana
Authors: Rascón Luis , Cruz Mario , Rodríguez-Jasso Rosa M. , Neira-Vielma Alberto A. , Ramírez-Barrón Sonia N. , Belmares Ruth
Published: December 2020

The use of ohmic heating (OH) processing technologies in beverages might provide a higher quality value to the final product; consumers tended to prefer more natural products with minimum preservative substances. The aim of this work was to evaluate the effect of OH over the presence of microorganisms in “aguamiel” as well as to study the effects on physicochemical analysis like total sugars, soluble solids, electric conductivity pH, and color. The results showed that the conductivity of “aguamiel” was 0.374 s/m, this as temperature increased, conductivity rose as well. During OH a bubbling was observed when reaching 70 °C due to the generation of electrochemical reactions during the OH process. OH had a significant effect in the reduction of E. coli, yeast, and lactobacillus compared to conventional pasteurization, reaching optimal conditions for its total inactivation. Regarding its physicochemical properties, both treatments, conventional pasteurization and OH, did not show negative changes in aguamiel, demonstrating that OH technology can be a feasible option as a pasteurization technique. In conclusion it is important to notice that negative changes were not found in quality, color and sugars of “aguamiel”. Therefore, ohmic heating can be an option to replace traditional methods used for pasteurization.
Anomalous Sun Flyby of 1I/2017 U1 (`Oumuamua)
Authors: Wilhelm Klaus , Dwivedi Bhola
Published: December 2020

The findings of Micheli et al. (Nature2018, 559, 223–226) that 1I/2017 U1 (`Oumuamua) showed anomalous orbital accelerations have motivated us to apply an impact model of gravity in search for an explanation. A small deviation from the 1/r potential, where r is the heliocentric distance, is expected for the gravitational interaction of extended bodies as a consequence of this model. This modification of the potential results from an offset of the effective gravitational centre from the geometric centre of a spherically symmetric body. Applied to anomalous Earth flybys, the model accounts for energy gains relative to an exact Kepler orbit and an increased speed of several spacecraft. In addition, the flat rotation profiles of eight disk galaxies could be explained, as well as the anomalous perihelion advances of the inner planets and the asteroid Icarus. The solution in the case of `Oumuamua is also based on the proposal that the offset leads to an approach and flyby trajectory different from a Kepler orbit without postulating cometary activity. As a consequence, an adjustment of the potential and centrifugal orbital energies can be envisaged outside the narrow uncertainty ranges of the published post-perihelion data without a need to re-analyse the original data. The observed anomalous acceleration has been modelled with respect to the orbit solutions JPL 16 and “Pseudo-MPEC” for 1I/`Oumuamua.
A Novel Amino Acid Substitution, Fibrinogen Bβp.Pro234Leu, Associated with Hypofibrinogenemia Causing Impairment of Fibrinogen Assembly and Secretion
Authors: Kaido Takahiro , Yoda Masahiro , Kamijo Tomu , Arai Shinpei , Taira Chiaki , Higuchi Yumiko , Okumura Nobuo
Published: December 2020

We identified a novel heterozygous variant, Bβp.Pro234Leu (fibrinogen Tokorozawa), which was suspected to be associated with hypofibrinogenemia. Therefore, we analyzed the assembly and secretion of this fibrinogen using Chinese hamster ovary (CHO) cells. To determine the impact on the synthesis and secretion of fibrinogen of the Bβp.P234L and γp.G242E substitutions, we established recombinant variant fibrinogen-producing CHO cell lines. Synthesis and secretion analyses were performed using an enzyme-linked immunosorbent assay (ELISA) and immunoblotting analysis with the established cell lines. In addition, we performed fibrin polymerization using purified plasma fibrinogen and in-silico analysis. Both Bβp.P234L and γp.G242E impaired the secretion and synthesis of fibrinogen. Moreover, immunoblotting analysis elucidated the mobility migration of the Bβγ complex in Bβp.P234L. On the other hand, the fibrin polymerization of fibrinogen Tokorozawa was similar to that of normal fibrinogen. In-silico analysis revealed that the Bβp.P234 residue is located in the contact region between the Bβ and γ chains and contacts γp.G242 residue. The present study demonstrated that the Bβp.P234L substitution resulted in hypofibrinogenemia by decreasing the assembly and secretion of fibrinogen. Therefore, there is a possibility that substitutions in the contact region between the Bβ and γ chains impact the assembly and secretion of fibrinogen.
Systematic Review and Meta-Analysis on Hysterectomy by Vaginal Natural Orifice Transluminal Endoscopic Surgery (vNOTES) Compared to Laparoscopic Hysterectomy for Benign Indications
Authors: Housmans Susanne , Noori Nargis , Kapurubandara Supuni , Bosteels Jan J. A. , Cattani Laura , Alkatout Ibrahim , Deprest Jan , Baekelandt Jan
Published: December 2020

(1) Objective: We aimed to report an update of the systematic review and meta-analysis by Baekelandt et al. (2016). (2) Method: We followed PRISMA guidelines to perform this systematic review. We searched MEDLINE, EMBASE, CENTRAL and additional sources and aimed to retrieve randomised controlled trials (RCTs), controlled clinical trials (CCTs) and prospective/retrospective cohort studies in human subjects that allowed direct comparison of vNOTES to laparoscopy. (3) Results: Our search yielded one RCT and five retrospective cohort trials. Pooled analysis of two subgroups showed that, compared to conventional laparoscopy, vNOTES is equally effective to successfully remove the uterus in individuals meeting the inclusion criteria. vNOTES had significantly lower values for operation time, length of stay and estimated blood loss. There was no significant difference in intra- and postoperative complications, readmission, pain scores at 24 h postoperative and change in hemoglobin (Hb) on day 1 postoperative.
Clinical Outcomes after Additional Dynamic Renal® Stent Implantation for Stent Recoil in Ostial Coronary Lesions
Authors: Abdulrahman Bachir , Mashayekhi Kambis , Tajti Péter , Ferenc Miroslaw , Valina Christian Marc , Hochholzer Willibald , Neumann Franz-Josef , Nührenberg Thomas Georg
Published: December 2020

Background: Interventional treatment of aorto-ostial coronary stenoses is limited by stent recoil and suboptimal angiographic results, leading to restenosis and frequent re-interventions. As a potential bail-out strategy for stent recoil, implantation of an additional stent to increase radial force has been reported. Thus, we sought to investigate clinical outcomes after additional implantation of a Dynamic Renal® stent (DRS), a non-coronary; bare-metal stent with very high radial force, in aorto-ostial coronary stenoses. Methods: Patients treated by implantation of DRSs for stent recoil in the ostial right coronary artery or the left main stem were identified from the hospital database. Baseline clinical and procedural characteristics were compared to patients who underwent re-intervention for in-stent-restenosis in similar segments by either implantation of conventional drug-eluting stents (DES) or paclitaxel-coated balloons (PCB). Clinical follow-ups were performed up to three years following re-intervention with the assessment of death, target lesion reintervention (TLR), and major adverse cardiac events (MACE) as a combination death, myocardial infarction and target vessel revascularization. Kaplan–Meier analyses were performed for event-free survival between the three groups. Results: Between 05/2013 and 07/2019, 28 patients underwent DRS implantation of aorto-ostial coronary lesions. In comparison with 49 patients with DES implantation and 29 patients undergoing PCB treatment, no relevant differences in baseline parameters were identified. Median follow-up was 714 days, with an available follow-up of >1 year after intervention in 82.1% of patients. In the entire study cohort at two years after re-intervention, the TLR rate was 16% (17 patients), the MACE rate 37% (39 patients), and all-cause mortality 9% (10 patients), with no significant differences between the three groups. Conclusions: DRS implantation for treating stent recoil of aorto-ostial coronary lesions resulted in a high rate of TLR, and was associated with similar risk for death and MACE compared to treatment of in-stent-restenosis with DES or PCB. Randomized, larger comparisons of contemporary DES in patients exclusively presenting with stent recoil are necessary to further define the efficacy and safety of this approach.
Association between Multidimensional Prognostic Index and Hospitalization and Mortality among Older Adults with Chronic Kidney Disease on Conservative or on Replacement Therapy
Authors: Lai Silvia , Amabile Maria , Mazzaferro Sandro , Imbimbo Giovanni , Mitterhofer Anna , Galani Alessandro , Aucella Filippo , Brunori Giuliano , Menè Paolo , Molfino Alessio ,   The Study Group on Geriatric Nephrology of the Italian Society of Nephrology (SIN)
Published: December 2020

The prevalence of renal disease is constantly increasing in older adults and a prognostic evaluation by a valid tool may play a key role in treatment management. We aimed to assess the association(s) between the multidimensional prognostic index (MPI) and both the hospitalization and mortality among older adults with renal disease. Patients with chronic kidney disease (CKD) (stage 3–5 KDOQI) and on dialysis were considered. Clinical parameters were registered at baseline and after 2 years. In all the patients, the MPI was calculated and divided into grade 0 (low risk), 1 (moderate risk), and 2 (severe risk). Hospitalizations and mortality were recorded during the follow-up and analyzed according to MPI grade. A total of 173 patients, with a median age of 76 years, on conservative (n = 105) and replacement therapy (32 patients on hemodialysis, 36 patients on peritoneal dialysis) were enrolled. Of them, 60 patients were in MPI grade 0, 102 in grade 1, and 11 in grade 2. The median duration of all the hospitalizations was 6 days and the number of deaths was 33. MPI significantly correlated with days of hospitalization (r = 0.801, p < 0.00001) and number of hospitalizations per year (r = 0.808, p < 0.00001), which was higher in MPI grade 2 compared to grade 1 (p < 0.001) and to grade 0 (p < 0.001). We found a significant association between MPI grades and mortality (p < 0.001). Our results indicate that MPI was associated with outcomes in patients with renal disease, suggesting that a multidimensional evaluation should be implemented in this clinical setting.
Treatment of Heart Failure Patients with Anxiolytics Is Associated with Adverse Outcomes, with and without Depression
Authors: Zwas Donna R. , Keren Andre , Amir Offer , Gotsman Israel
Published: December 2020

Background: Few studies have evaluated the effect of pharmacologic treatment of anxiety on outcomes in heart failure (HF) patients. This study examined the impact of treatment with anxiolytics on clinical outcomes in a real-world sample of HF patients with and without depression. Methods: Patients diagnosed with HF were retrieved from a large HMO database. Patients prescribed anxiolytic medication and patients diagnosed with depression and/or prescribed anti-depressant medication were followed for cardiac-related hospitalizations and death. Results: The study cohort included 6293 HF patients. Treatment with anxiolytics was associated with decreased one-year survival compared to untreated individuals, with a greater reduction in survival seen in patients diagnosed with depression and/or treated with anti-depressants. Multi-variable analysis adjusting for age, sex, NYHA class, cardiac risk factors and laboratory parameters found that treatment with anxiolytics remained a predictor of mortality even when adjusting for depression. Depression combined with anxiolytic treatment was predictive of increased mortality, and treatment with anxiolytics alone, depression alone and anxiolytic treatment together with depression were each associated with an increased hazard ratio for a composite outcome of death and hospitalization. Conclusions: In this real-world study of HF patients, both treatment with anxiolytics and depression were associated with increased mortality, and anxiolytic therapy remained a predictor of mortality when adjusting for depression. Treatment of anxiety together with depression was associated with the highest risk of mortality. Safe and effective treatment for anxiety and depression is warranted to alleviate the detrimental impact of these disorders on quality and of life and adverse events.
Fibrosis Distinguishes Critical Limb Ischemia Patients from Claudicants in a Transcriptomic and Histologic Analysis
Authors: Cong Guangzhi , Cui Xiangdong , Ferrari Ricardo , Pipinos Iraklis I. , Casale George P. , Chattopadhyay Ansuman , Sachdev Ulka
Published: December 2020

Most patients with critical limb ischemia (CLI) from peripheral arterial disease (PAD) do not have antecedent intermittent claudication (IC). We hypothesized that transcriptomic analysis would identify CLI-specific pathways, particularly in regards to fibrosis. Derivation cohort data from muscle biopsies in PAD and non-PAD (controls) was obtained from the Gene Expression Omnibus (GSE120642). Transcriptomic analysis indicated CLI patients (N = 16) had a unique gene expression profile, when compared with non-PAD controls (N = 15) and IC (N = 20). Ninety-eight genes differed between controls and IC, 2489 genes differed between CLI and controls, and 2783 genes differed between CLI and IC patients. Pathway enrichment analysis showed that pathways associated with TGFβ, collagen deposition, and VEGF signaling were enriched in CLI but not IC. Receiver operating curve (ROC) analysis of nine fibrosis core gene expression revealed the areas under the ROC (AUC) were all >0.75 for CLI. Furthermore, the fibrosis area (AUC = 0.81) and % fibrosis (AUC = 0.87) in validation cohort validated the fibrosis discrimination CLI from IC and control (all n = 12). In conclusion, transcriptomic analysis identified fibrosis pathways, including those involving TGFβ, as a novel gene expression feature for CLI but not IC. Fibrosis is an important characteristic of CLI, which we confirmed histologically, and may be a target for novel therapies in PAD.
Nightmares in Patients with Major Depressive Disorder, Bipolar Disorder, and Psychotic Disorders: A Systematic Review
Authors: Akkaoui Marine Ambar , Lejoyeux Michel , d’Ortho Marie-Pia , Geoffroy Pierre A.
Published: December 2020

Chronic nightmares are very common in psychiatric disorders, affecting up to 70% of patients with personality or post-traumatic stress disorders. In other psychiatric disorders, the relationships with nightmares are poorly known. This review aimed to clarify the relationship between nightmares and both mood and psychotic disorders. We performed a systematic literature search using the PubMed, Cochrane Library and PsycINFO databases until December 2019, to identify studies of patients suffering from either a mood disorder or a psychotic disorder associated with nightmares. From the 1145 articles screened, 24 were retained, including 9 studies with patients with mood disorders, 11 studies with patients with psychotic disorders and 4 studies with either psychotic or mood disorders. Nightmares were more frequent in individuals with mood or psychotic disorders than in healthy controls (more than two-fold). Patients with frequent nightmares had higher suicidality scores and had more frequently a history of suicide attempt. The distress associated with nightmares, rather than the frequency of nightmares, was associated with the severity of the psychiatric disorder. Further studies assessing whether nightmare treatment not only improves patient–sleep perception but also improves underlying psychiatric diseases are needed. In conclusion, nightmares are overrepresented in mood and psychotic disorders, with the frequency associated with suicidal behaviors and the distress associated with the psychiatric disorder severity. These findings emphasize major clinical and therapeutic implications.
Generic and Respiratory-Specific Quality of Life in Non-Hospitalized Patients with COVID-19
Authors: Meys Roy , Delbressine Jeannet M. , Goërtz Yvonne M.J. , Vaes Anouk W. , Machado Felipe V.C. , Van Herck Maarten , Burtin Chris , Posthuma Rein , Spaetgens Bart , Franssen Frits M.E. , Spies Yvonne , Vijlbrief Herman , van’t Hul Alex J. , Janssen Daisy J.A. , Spruit Martijn A. , Houben-Wilke Sarah
Published: December 2020

The impact of coronavirus disease 2019 (COVID-19) on quality of life appears to be highly underestimated, especially in patients who have not been admitted to the hospital. Therefore, our aim was to assess respiratory-specific quality of life in addition to generic quality of life in former patients with confirmed/suspected COVID-19 who have never been admitted to the hospital. Members of an online Belgian social support group for patients with confirmed/suspected COVID-19 with persistent complaints, completed an online survey. The five-level EQ-5D (EQ-5D-5L) and the Clinical COPD Questionnaire (CCQ) were used to assess generic and respiratory-specific quality of life, respectively. Data of 210 non-hospitalized patients (88% women, 45 ± 11 years, 79 ± 17 days after symptom onset) were included in the analyses. Mean EQ-5D index and visual analogue scale (EQ-VAS) score was 0.62 ± 0.19 and 50.71 ± 18.87, respectively, with 40% of the patients demonstrating an EQ-5D index that was below the fifth percentile of normative values, indicating poor generic quality of life. The mean CCQ score was 2.01 ± 0.98 points, while 123 respondents (59%) had a total score ≥1.9 points, indicating poor respiratory-specific quality of life. The correlation between EQ-5D index score/EQ-VAS score and CCQ total score was moderate (r = −0.524 and r = −0.374; both p < 0.001). In conclusion, both generic and respiratory-specific quality of life are affected in non-hospitalized patients with COVID-19, approximately three months after the onset of symptoms. The combined use of the EQ-5D and the CCQ could identify the broad impact of COVID-19 on quality of life.
Pars Plana Vitrectomy and the Risk of Ocular Hypertension and Glaucoma: Where Are We?
Authors: Rossi Tommaso , Ripandelli Guido
Published: December 2020

Purpose is to review the pathogenic mechanism for ocular hypertension and glaucoma development after pars plana vitrectomy. Both acute and chronic causes are considered, and special attention is paid to the theories and clinical evidence on the risk of developing Open Angle Glaucoma (OAG) after Pars Plana Vitrectomy (PPV). Most existing scientific literature on the issue agree on the role of ascorbate as an oxygen scavenger within the vitreous chamber. Oxygen tension in the vitreous and anterior chamber is maximum inn proximity of the retinal surface and endothelium, respectively and steeply decreases toward the lens, on both sides, and trabecular meshwork. Vitreous removal and, to a lesser extent, liquefaction, greatly reduces oxygen tension gradient in vitreous chamber while cataract extraction has similar effects on anterior chamber oxygen gradients. Oxygen derivatives originated from the cornea and retina are actively reduced by the vitreous gel and/or the crystalline lens. Vitreous removal and cataract extraction reduce drastically this function. Most reported clinical series confirm this hypothesis although protocol difference and follow-up length greatly impact the reliability of results.
The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome
Authors: Buczyńska Angelika , Sidorkiewicz Iwona , Ławicki Sławomir , Krętowski Adam , Zbucka-Krętowska Monika
Published: December 2020

Prenatal screening for Down syndrome (DS) is based on both noninvasive and invasive methods. Noninvasive, cell-free fetal DNA genetic tests are expensive, whereas biochemical methods remain imprecise. Amniocentesis is the most frequently used invasive diagnosis procedure, characterized by 99.8% diagnostic efficiency and less than 1% risk of miscarriage. The aim of this study was to evaluate the screening value of apolipoprotein E (ApoE) as a potential noninvasive biomarker for prenatal DS assessment. This study was conducted on a group of female patients who decided to undergo routine amniocentesis between the 15th and 18th week of pregnancy at the Department of Reproduction and Gynecological Endocrinology of the Medical University of Bialystok, Poland. For the purpose of this study, 20 women with DS fetuses were selected as the study group, and 20 healthy pregnant women with euploid fetus karyotypes as the control group. The plasma levels of ApoE were significantly higher in the study group compared to healthy subjects (p < 0.05). The area under the receiver operating characteristic (ROC) curve was 0.978 (p < 0.001), with the cut-off set to 1.37 mg/mL, which was characterized by 80% of sensitivity and 100% of specificity. The high sensitivity and specificity demonstrate the screening utility of maternal ApoE concentration in prenatal fetal DS screening.
Primary Breast Extranodal Marginal Zone Lymphoma in Primary Sjögren Syndrome: Case Presentation and Relevant Literature
Authors: Ingravallo Giuseppe , Maiorano Eugenio , Moschetta Marco , Limongelli Luisa , Mastropasqua Mauro Giuseppe , Agazzino Gisella Franca , De Ruvo Vincenzo , Tarantino Paola , Favia Gianfranco , Capodiferro Saverio
Published: December 2020

The association between autoimmune diseases, mostly rheumatoid arthritis, systemic lupus erythematosus, celiac disease and Sjögren syndrome, and lymphoma, has been widely demonstrated by several epidemiologic studies. By a mechanism which has not yet been entirely elucidated, chronic activation/stimulation of the immune system, along with the administration of specific treatments, may lead to the onset of different types of lymphoma in such patients. Specifically, patients affected by Sjögren syndrome may develop lymphomas many years after the original diagnosis. Several epidemiologic, hematologic, and histological features may anticipate the progression from Sjögren syndrome into lymphoma but, to the best of our knowledge, a definite pathogenetic mechanism for such progression is still missing. In fact, while the association between Sjögren syndrome and non-Hodgkin lymphoma, mostly extranodal marginal zone lymphomas and, less often, diffuse large B-cell, is well established, many other variables, such as time of onset, gender predilection, sites of occurrence, subtype of lymphoma, and predictive factors, still remain unclear. We report on a rare case of primary breast lymphoma occurring three years after the diagnosis of Sjögren syndrome in a 57-year-old patient. The diagnostic work-up, including radiograms, core needle biopsy, and histological examination, is discussed, along with emerging data from the recent literature, thus highlighting the usefulness of breast surveillance in Sjögren syndrome patients.
Full Dilatation Caesarean Section and the Risk of Preterm Delivery in a Subsequent Pregnancy: A Historical Cohort Study
Authors: Ewington Lauren Jade , Quenby Siobhan
Published: December 2020

Full dilatation caesarean sections (CS) have increased risk of uterine extensions, which leads to cervical trauma that has been associated with an increased risk of spontaneous preterm birth (sPTB) in a subsequent pregnancy. The aim of this study was to determine if CS at full dilatation increased the risk of sPTB in a subsequent pregnancy in our unit. A historical cohort study was performed on women delivered by emergency CS between 2008–2015 (n = 5808) in a university hospital who had a subsequent pregnancy in this time frame (n = 1557). Women were classified into two exposure groups; those who were 6–9 cm and those fully dilated at index CS. The reference group was CS at 0–5 cm dilated. The primary outcome was sPTB < 37 weeks’ gestation. CS at 6–9 cm or fully dilated did not significantly increase the odds of sPTB in a subsequent pregnancy (aOR 1.64, 95% CI: 0.83–3.28, p = 0.158; aOR 1.86, 95% CI: 0.91–3.83; p = 0.090, respectively). However, a short interpregnancy interval of <1 year significantly increased the odds of sPTB in a subsequent pregnancy (aOR 3.10, 95% CI: 1.71–5.61). This study has found a short interpregnancy interval following a CS conferred a higher risk of sPTB than full dilatation CS. This finding highlights postnatal contraception and increased surveillance of women with short interpregnancy interval post CS as possible interventions to reduce sPTB.
Investigating Changes in Aeolian Sediment Transport at Coastal Dunes and Sand Trapping Fences: A Field Study on the German Coast
Authors: Eichmanns Christiane , Schüttrumpf Holger
Published: December 2020

For the restoration and maintenance of beach and dune systems along the coast, knowledge of aeolian sediment transport and its interaction with coastal protection measures is required. As a nature-based solution, sand trapping fences can be an integral part of coastal protection measures initiating foredune development. There are few detailed studies on aeolian sediment transport rates on coastal dunes and sand trapping fences available to date. Thus, in this work, we present the results of field experiments conducted at the beach, coastal dune, and sand trapping fence on the East Frisian island Langeoog. The vertical sediment flux profile was measured by vertical mesh sand traps, and saltiphones measured the instantaneous sediment transport. A meteorological station was set up to obtain wind data. On the beach, dune toe, and dune crest, the stationary wind profile can be described well by the law of the wall. Saturated aeolian sediment transport rates on the beach and dune toe were predicted by widely used empirical models. Between the sand trapping fence, these empirical transport models could not be applied, as no logarithmic wind profile existed. The upwind sediment supply reduced after each brushwood line of the sand trapping fence, thereby, leading to increased deviation from the saturated conditions.
Spatiotemporal Dynamics of Cultivated Land and Its Influences on Grain Production Potential in Hunan Province, China
Authors: Yu De , Hu Shougeng , Tong Luyi , Xia Cong
Published: December 2020

Understanding the impact of changes in cultivated land in terms of structure, distribution, and quantity on grain production potential (GPP) is essential for a sustainable land utilization strategy and food security. Cultivated land balance (CLB), as a critical policy aiming at protecting farmland in China, has greatly restricted the loss of cultivated land. However, changes in cultivated land were largely generated due to the land-use activities led by the CLB policy. To clarify how the spatiotemporal dynamics of cultivated land led by the CLB policy affects the GPP, this work discusses the impact mechanism of cultivated land changes on GPP and provides an empirical analysis in Hunan Province, China. This study shows that the activities that merely aim at restricting the loss of cultivated land under CLB cannot stop the decline in GPP in China, since it requires the government to reclaim a certain amount of cultivated land that is equal to that occupied for non-cultivated land use. Furthermore, the distribution of cultivated land changed after the implementation of CLB and, as a result, contributed to the decrease in the quality of cultivated land and GPP. Quantity, productivity, and other elements that may potentially facilitate cultivated land protection are greatly advocated to be considered to enrich the connotation of the CLB policy in China. It also found that less developed regions located in central and western Hunan Province, among other areas, observed a higher sensitivity of GPP to cultivated land change. More attention should be paid to protecting cultivated land in these regions and addressing issues such as the abandonment of high-quality cultivated land.
Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production
Authors: Askitosari Theresia D. , Berger Carola , Tiso Till , Harnisch Falk , Blank Lars M. , Rosenbaum Miriam A.
Published: December 2020

Sufficient supply of oxygen is a major bottleneck in industrial biotechnological synthesis. One example is the heterologous production of rhamnolipids using Pseudomonas putida KT2440. Typically, the synthesis is accompanied by strong foam formation in the reactor vessel hampering the process. It is caused by the extensive bubbling needed to sustain the high respirative oxygen demand in the presence of the produced surfactants. One way to reduce the oxygen requirement is to enable the cells to use the anode of a bioelectrochemical system (BES) as an alternative sink for their metabolically derived electrons. We here used a P. putida KT2440 strain that interacts with the anode using mediated extracellular electron transfer via intrinsically produced phenazines, to perform heterologous rhamnolipid production under oxygen limitation. The strain P. putida RL-PCA successfully produced 30.4 ± 4.7 mg/L mono-rhamnolipids together with 11.2 ± 0.8 mg/L of phenazine-1-carboxylic acid (PCA) in 500-mL benchtop BES reactors and 30.5 ± 0.5 mg/L rhamnolipids accompanied by 25.7 ± 8.0 mg/L PCA in electrode containing standard 1-L bioreactors. Hence, this study marks a first proof of concept to produce glycolipid surfactants in oxygen-limited BES with an industrially relevant strain.
Spent Coffee Grounds Alter Bacterial Communities in Latxa Dairy Ewes
Authors: Goiri Idoia , Díaz de Otálora Xabier , Ruiz Roberto , Rey Jagoba , Atxaerandio Raquel , Lavín Jose Luis , San Martin David , Orive Mikel , Iñarra Bruno , Zufia Jaime , Urkiza Jabi , García-Rodríguez Aser
Published: December 2020

Antimicrobial and antioxidant properties of spent coffee grounds (SCG) make them a potential ingredient in a diet for ruminants. This study investigated the effects of SCG on rumen microbiota. For 51 days, 36 dairy ewes were assigned to the experimental treatments (0, 30, 50, and 100 g SCG/kg). Ruminal samples were collected on day 50. DNA was extracted and subjected to paired-end Illumina sequencing of the V3-V4 hypervariable region of the 16S rRNA genes. Bioinformatic analyses were performed using QIIME (v.1.9.0). SCG increased dose-dependently bacterial diversity and altered bacterial structure. Further, 60, 78, and 449 operational taxonomic unit (OUT) were different between control and 30, 50 and 100 g/kg SCG groups, respectively. Higher differences were observed between the control and 100 g/kg SCG group, where OTU of the genera Treponema, CF231, Butyrivibrio, BF331, Anaeroplasma, Blautia, Fibrobacter, and Clostridium were enriched with SCG. Correlations between volatile fatty acids (VFA) and bacterial taxa were sparser in the SCG groups and had little overlap. Certain bacterial taxa presented different signs of the correlation with VFA in SCG and control groups, but Butyrivibrio and Blautia consistently correlated with branched-chain VFA in all groups. SCG induced shifts in the ruminal bacterial community and altered the correlation networks among bacterial taxa and ruminal VFA.
Investigation of In-Vitro Adaptation toward Sodium Bituminosulfonate in Staphylococcus aureus
Authors: Blisse Marko , Idelevich Evgeny A. , Becker Karsten
Published: December 2020

The global increase in antimicrobial resistance has revived the interest in “old” substances with antimicrobial activity such as sodium bituminosulfonate. However, for those “old” compounds, scientific studies are still sparse and the ones available do not mostly meet the current standards. Since this compound is used for topical applications, investigation of a potential increase in minimal inhibitory concentrations (MICs) is of particular importance. For selection of phenotypes with decreased susceptibility, a collection of 30 genetically diverse methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains were cultured on bi-layered linear gradient agar plates containing sub-inhibitory concentrations of the active agents. The stability of phenotypes with increased MICs was determined by serial passage on agent-free medium. Within 10 passages, only slight and, in most cases, reversible increases in MSSA and MRSA MIC levels toward sodium bituminosulfonate were obtained. Fusidic acid, used as a control, showed exponential expansions in MIC based on mutations in the fusA gene (elongation factor G or EF-G) with no reduction during the recovery phase. The only marginal and largely reversible changes of S. aureus MICs after exposition to sodium bituminosulfonate indicate a low potential for resistance development.