bluuebunny/crossref_metadata_2025 huggingface dataset:
Deoxynivalenol Detection beyond the Limit in Wheat Flour Based on the Fluorescence Hyperspectral Imaging Technique
Authors: Wang Chengzhi , Fu Xiaping , Zhou Ying , Fu Feng
Published: March 2024

Deoxynivalenol (DON) is a harmful fungal toxin, and its contamination in wheat flour poses a food safety concern globally. This study proposes the combination of fluorescence hyperspectral imaging (FHSI) and qualitative discrimination methods for the detection of excessive DON content in wheat flour. Wheat flour samples were prepared with varying DON concentrations through the addition of trace amounts of DON using the wet mixing method for fluorescence hyperspectral image collection. SG smoothing and normalization algorithms were applied for original spectra preprocessing. Feature band selection was carried out by applying the successive projection algorithm (SPA), uninformative variable elimination (UVE), competitive adaptive reweighted sampling (CARS), and the random frog algorithm on the fluorescence spectrum. Random forest (RF) and support vector machine (SVM) classification models were utilized to identify wheat flour samples with DON concentrations higher than 1 mg/kg. The results indicate that the SG–CARS–RF and SG–CARS–SVM models showed better performance than other models, achieving the highest recall rate of 98.95% and the highest accuracy of 97.78%, respectively. Additionally, the ROC curves demonstrated higher robustness on the RF algorithm. Deep learning algorithms were also applied to identify the samples that exceeded safety standards, and the convolutional neural network (CNN) model achieved a recognition accuracy rate of 97.78% for the test set. In conclusion, this study demonstrates the feasibility and potential of the FHSI technique in detecting DON infection in wheat flour.
The Effect of the Periodic Drying Method on the Drying Time of Hazelnuts and Energy Utilization
Authors: Akgün Mithat , Kontaş Emrullah
Published: March 2024

Hazelnut is a shelled fruit that is stored by drying and used as a snack or in industry. Since the hazelnut drying process is energy-intensive, there is a need for drying methods that will reduce the energy cost without lengthening the drying time. In this study, the effects of periodic drying of hazelnuts’ energy recovery, oil, and protein content, as well as mass losses, were studied. Fresh Tombul hazelnuts (Corylus avellana L.) with a diameter of Ø 15–16 mm were dried in a tunnel dryer over 16 different periods by adjusting the drying time inside and waiting time outside the oven until the moisture content reached 6%. Drying experiments were carried out at 45 °C and three different air velocities. The increase in air velocity resulted in a reduction in the periodic drying time between 10% and 36%. The optimum drying in terms of drying time and energy utilization was realized at 0.5 m/s air velocity, with a 1.5 h working time and 0.5 h waiting time. During this period, drying time increased by 19% and energy utilization was 69%. For periodic drying, the increase in oven working time causes a decrease in energy utilization, while the increase in waiting time causes an increase in energy utilization and drying time. Periodic drying had no negative effect on hazelnut oil and protein content. Periodic drying is a suitable option for saving energy during hazelnut drying.
Utilizing a Fractional-Order Grey Model to Predict the Development Trends of China’s Electronic Commerce Service Industry
Authors: Guo Jianhong , Chang Che-Jung , Huang Yingyi
Published: March 2024

Electronic commerce plays a vital role in the digital age, and the creation of a good electronic commerce ecosystem is crucial to maintaining economic growth. The electronic commerce service industry is a leading indicator of electronic commerce development, and its possible changes imply the future trends and innovation directions of the electronic commerce industry. An accurate grasp of the possible future revenue scale of the electronic commerce service industry can provide decision-making information for government policy formulation. Electronic commerce companies must formulate operational plans based on the latest information to determine strategic directions that are reasonable and consistent with the actual situation. Although there exist many prediction methods, they often fail to produce ideal results when the number of observations is insufficient. The fractional-order grey model is a common method used to deal with small data set prediction problems. This study therefore proposes a new modeling procedure for the fractional-order grey model to predict the revenue scale of China’s electronic commerce service industry. The results of experiments demonstrate that the proposed procedure can yield robust outputs under the condition of small data sets to reduce decision-making risks. Therefore, it can be regarded as a practical small data set analysis tool for managers.
Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids
Authors: Ribeiro João , Luís Marco Â. , Rodrigues Bruno , Santos Fátima Milhano , Mesquita Joana , Boto Renato , Tomaz Cândida Teixeira
Published: March 2024

The increasing demand for highly pure biopharmaceuticals has put significant pressure on the biotechnological industry to innovate in production and purification processes. Nucleic acid purification, crucial for gene therapy and vaccine production, presents challenges due to the unique physical and chemical properties of these molecules. Meeting regulatory standards necessitates large quantities of biotherapeutic agents of high purity. While conventional chromatography offers versatility and efficiency, it suffers from drawbacks like low flow rates and binding capacity, as well as high mass transfer resistance. Recent advancements in continuous beds, including monoliths and cryogel-based systems, have emerged as promising solutions to overcome these limitations. This review explores and evaluates the latest progress in chromatography utilizing monolithic and cryogenic supports for nucleic acid purification.
Advancements in Viral Gene Therapy for Gaucher Disease
Authors: Kulkarni Akhil , Chen Tiffany , Sidransky Ellen , Han Tae-Un
Published: March 2024

Gaucher disease, an autosomal recessively inherited lysosomal storage disorder, results from biallelic mutations in the GBA1 gene resulting in deficient activity of the enzyme glucocerebrosidase. In Gaucher disease, the reduced levels and activity of glucocerebrosidase lead to a disparity in the rates of formation and breakdown of glucocerebroside and glucosylsphingosine, resulting in the accumulation of these lipid substrates in the lysosome. This gives rise to the development of Gaucher cells, engorged macrophages with a characteristic wrinkled tissue paper appearance. There are both non-neuronopathic (type 1) and neuronopathic (types 2 and 3) forms of Gaucher disease, associated with varying degrees of severity. The visceral and hematologic manifestations of Gaucher disease respond well to both enzyme replacement therapy and substrate reduction therapy. However, these therapies do not improve the neuronopathic manifestations, as they cannot cross the blood–brain barrier. There is now an established precedent for treating lysosomal storage disorders with gene therapy strategies, as many have the potential to cross into the brain. The range of the gene therapies being employed is broad, but this review aimed to discuss the progress, advances, and challenges in developing viral gene therapy as a treatment for Gaucher disease.
Overexpression of NtGPX8a Improved Cadmium Accumulation and Tolerance in Tobacco (Nicotiana tabacum L.)
Authors: Peng Xiang , Ma Tengfei , Song Kejin , Ji Xue , Xiang Lien , Chen Nan , Zu Ronglei , Xu Wenyi , Zhu Shunqin , Liu Wanhong
Published: March 2024

Cadmium (Cd)-induced oxidative stress detrimentally affects hyperaccumulator growth, thereby diminishing the efficacy of phytoremediation technology aimed at Cd pollution abatement. In the domain of plant antioxidant mechanisms, the role of glutathione peroxidase (GPX) in conferring Cd tolerance to tobacco (Nicotiana tabacum) remained unclear. Our investigation employed genome-wide analysis to identify 14 NtGPX genes in tobacco, revealing their organization into seven subgroups characterized by analogous conserved domain patterns. Notably, qPCR analysis highlighted NtGPX8a as markedly responsive to Cd2+ stress. Subsequent exploration through yeast two-hybridization unveiled NtGPX8a’s utilization of thioredoxins AtTrxZ and AtTrxm2 as electron donors, and without interaction with AtTrx5. Introduction of NtGPX8a into Escherichia coli significantly ameliorated Cd-induced adverse effects on bacterial growth. Transgenic tobacco overexpressing NtGPX8a demonstrated significantly augmented activities of GPX, SOD, POD, and CAT under Cd2+ stress compared to the wild type (WT). Conversely, these transgenic plants exhibited markedly reduced levels of MDA, H2O2, and proline. Intriguingly, the expression of NtGPX8a in both E. coli and transgenic tobacco led to increased Cd accumulation, confirming its dual role in enhancing Cd tolerance and accumulation. Consequently, NtGPX8a emerges as a promising candidate gene for engineering transgenic hyperaccumulators endowed with robust tolerance for Cd-contaminated phytoremediation.
Effects of Static Meditation Practice on Blood Lipid Levels: A Systematic Review and Meta-Analysis
Authors: Antonelli Michele , Donelli Davide , Gurgoglione Filippo Luca , Lazzeroni Davide , Halasz Geza , Niccoli Giampaolo
Published: March 2024

This review aims to delineate the potential impact of static meditation practice on cholesterol and triglyceride levels. PubMed, EMBASE, Web of Science, Cochrane Library, and Google Scholar were systematically screened up until December 2023 to identify pertinent studies. After searching the scientific literature, 16 clinical studies (11 trials and 5 observational experiments) met the criteria for inclusion, involving a total of 1147 participants. In general, Ayurvedic-based meditation techniques were predominantly associated with lower total cholesterol levels, mindfulness-based techniques demonstrated benefits in both total cholesterol and triglyceride levels, and Eastern meditation techniques with spiritual origins were primarily linked to improved serum concentrations of HDL cholesterol. Study participants mostly engaged in meditation on a daily basis, often practicing it once or even twice a day, spanning a duration ranging from a few weeks to several months. The meta-analysis shows an association between meditation practice in healthy or sub-healthy adults and reduced cholesterol levels, with an average decrease of approximately −14 mg/dL (MD = −13.91 [−23.35; −4.47] mg/dL; p = 0.02), alongside favorable and even more pronounced impacts on triglyceride levels (MD = −32.56 [−48.44; −16.68] mg/dL; p < 0.01). In summary, regular engagement in static meditation practices can be associated with lower triglyceride and, to a lesser extent, cholesterol levels. Further studies on the topic are recommended to better investigate the relationship between meditation practice and physiological parameters.
Sex Differences in the Impact of Dynapenic Abdominal Obesity on Mild Cognitive Impairment among Korean Elderly in the Community
Authors: Kim Jiyun , Paik Yuna
Published: March 2024

Abdominal obesity (AO) and dynapenia (DP) are associated with cognitive decline, and the relationship between dynapenic abdominal obesity (DAO), a combination of DP and AO, and mild cognitive impairment (MCI) has been confirmed. This study aims to determine whether this relationship exhibits potential sex differences. The relationship between MCI and DAO was confirmed in 1309 community elderly individuals aged 65 years or older who were not diagnosed with dementia. The MCI was defined as a Korean mini-mental state examination (K-MMSE) score of 18–23 points. Multiple logistic regression analyses were conducted, categorizing participants into groups: a control group without AO or DP, an AO group, a DP group, and a DAO group. The study results showed that in women, both DP and DAO were significantly associated with MCI not only in the unadjusted Model 1 but also in Model 2, which adjusted for general characteristics and health behaviors, and Model 3, which additionally adjusted for chronic diseases and disease-related characteristics. In men, DP was associated with MCI in the unadjusted Model 1. The findings highlight sex differences in the impact of the DAO on MCI. These differences should be considered when studying the factors related to MCI in old age.
Factors Influencing Injury Severity and Frequency among Korean Sports Participants in Their 20s and 30s
Authors: Kwon Jeonga , Jang Jusun
Published: March 2024

This study aimed to explore the factors that affect the severity and number of sports injuries among Korean sports participants in their 20s and 30s. The data of 5118 lifestyle and professional athletes in their 20s and 30s were collected from a sports safety survey conducted by the Korea Sports Safety Foundation in 2019. The characteristics of the study population were analyzed using descriptive analysis. Determinants of injury severity and frequency based on participants’ sex were identified using multivariate logistic regression analyses. The results show that among male sports participants, the type of athlete, knowledge of first aids, the physical condition, completing safety education, the skill level, and checking environmental factors were associated with injury severity. Among female sports participants, the type of athlete, the physical condition, performing finishing exercises, completing safety education, and the skill level were associated with injury severity. Additionally, among male sports participants, physical condition, completing safety education, skill level, participating in exercises according to one’s fitness level, and having an awareness of accident prevention methods were associated with injury frequency. Lastly, among female sports participants, drinking water during scheduled breaks, carrying water to stay hydrated, physical condition, completing safety education, and skill level were associated with injury frequency. Furthermore, being a lifestyle athlete, being in good physical condition, and having beginner or intermediate skills in one’s sport reduced injury severity among Korean sports participants in their 20s and 30s. Being in good physical condition, not completing safety education, and having beginner skills in one’s sport reduced the injury frequency among Korean sports participants in their 20s and 30s. These findings suggest that lifestyle and professional athletes should be aware of these factors and participate in sports activities in a manner that reduces injury severity and frequency. Moreover, these factors should be considered when developing strategies to prevent sport injuries in lifestyle and professional athletes and allow them to participate in sports activities safely.
Preoperative Risk Factors for Periprosthetic Joint Infection: A Narrative Review of the Literature
Authors: Lucenti Ludovico , Testa Gianluca , Caldaci Alessia , Sammartino Fabio , Cicio Calogero , Ilardo Martina , Sapienza Marco , Pavone Vito
Published: March 2024

Periprosthetic joint infection (PJI) poses a challenging complication for many patients undergoing arthroplasty, and the literature identifies numerous risk factors. A comprehensive understanding of the primary risk and protective factors for PJI is valuable for surgeons. This article aims to compile and summarize the key risk factors for PJI documented in the literature. Some risk factors are related to the nutritional status of patients, with obesity, weight loss, hypovitaminosis, and malnutrition being frequently reported. Pathologies affecting patients also contribute to PJI risk, including septic arthritis, hepatitis, diabetes, urinary tract infections, anemia, hypothyroidism, osteoporosis, and dental pathologies. Unhealthy habits, such as tobacco and drug abuse, are significant factors. Previous corticosteroid injections may also play a role in infection development. A few protective factors are also reported in the literature (use of statins, preoperative decolonization, and preadmission skin preparation). The identification of risk factors and the implementation of evidence-based preoperative protocols are essential steps in reducing the incidence of PJI.
Physical Properties and Crop Performance of Four Substrate Fibers in Greenhouse Petunia Production
Authors: Thiessen Maureen E. , Fields Jeb S. , Abdi Damon E.
Published: March 2024

As peat (P) demand increases throughout the horticultural industry, alternative fibers must be evaluated. Sugarcane bagasse (B), wood fiber (W), and coconut coir (C) have received interest as domestically available alternatives to P, with demonstrated success in producing greenhouse crops. However, there is limited research comparing these materials to peat. This research evaluated the substrate properties and productivity of Petunia Supertunia Mini Vista ‘Indigo’ in pine bark substrates amended with C, W, B, or P and fertigated weekly at 100, 200, or 300 parts per million (ppm) nitrogen (N) to account for possible N immobilization. The container capacity was lowest and air-filled porosity was highest in W and B substrates. Substrate pH increased in W and B substrates, and C substrates were fertigated at 100 ppm N. Increasing the N rate increased the growth index in all substrates, especially B and W substrates later in the production period. Higher fertilization increased shoot mass, chlorophyll content, and blooms across all substrates, demonstrating that fertilizer supplementation may offset possible N immobilization. While plant growth and quality parameters were greatest in the P blend, increasing N applications produced similar-quality plants using alternative substrates, demonstrating that modifying fertilizer management practices can make alternative fibers a viable horticultural substrate.
Genome-Wide Prediction and Expression Characterization of the GATA Gene Family under Nitrogen and Phosphate Deficiency in Panax ginseng
Authors: Liang Hao , Sun Hai , Shao Cai , Qian Jiaqi , Lv Bochen , Zhu Jiapeng , Zhang Yayu
Published: March 2024

GATA transcription factors are widespread in plants, exerting crucial functions in multiple processes such as flower development, photoperiod regulation, and light signal transduction. The GATA gene family has a key role in the regulation of medicinal plant adaptation to environmental stress. However, since the publication of the Ginseng (Panax ginseng C.A. Meyer) genome-wide data, there has never been an analysis of the whole GATA gene family. To understand the function of the GATA gene family more broadly, the GATA gene family members in P. ginseng were predicted using an in silico bioinformatics approach. A comprehensive and systematic analysis encompassing chromosome scaffold, expression pattern, gene structure, and phylogeny was conducted. The results showed that a total of 52 GATA gene family members were recognized in P. ginseng, distributed across 51 scaffolds. Each member encoded a diverse number of amino acid residues, extending from 138 to 1064. Moreover, the expression levels of PgGATA genes were significantly altered by nitrogen (N) and phosphorus (P) stresses. The expression levels of PgGATA6, PgGATA11, PgGATA27, PgGATA32, PgGATA37, PgGATA39, PgGATA40, and PgGATA50 exhibited significant elevation under N deficiency, whereas PgGATA15, PgGATA18, PgGATA34, PgGATA38, PgGATA41, and PgGATA44 genes showed substantial upregulation under P deficiency. In addition, PgGATA3, PgGATA4, PgGATA14, PgGATA19, and PgGATA28 were substantially upregulated under both N and P deficiency. This research establishes a theoretical foundation for the thorough examination of the functions of the PgGATA gene family and its regulation by N and P fertilization during P. ginseng cultivation.
Working Together to Support Self-Determination for Tāngata Kāpō (Blind and Low Vision) Māori: An Exemplar
Authors: Masters-Awatere Bridgette , Graham Rebekah , Cowan Chrissie
Published: March 2024

This paper addresses the marginalisation of tāngata kāpō Māori (blind and low-vision Indigenous New Zealanders) in health- and vision-related research, despite New Zealand’s commitments to international conventions. Utilising a pūrākau-based approach, it challenges existing colonial narratives and emphasises the importance of Māori perspectives. We advocate for Māori self-determination over research processes. This paper shares insights from a systematic review and the development of a declaration for engaging with tāngata kāpō Māori, reflecting the 3-year collaborative process. The Materials and Methods section details a Kaupapa Māori-grounded data collection, prioritising relationships and cultural practices. Feedback loops with participants and forums ensure accurate representation. In conclusion, the study underscores NZ government obligations and presents the “3Rs” framework—relationships, respect, and reciprocity—as essential for meaningful research engagements with tāngata kāpō Māori. The findings contribute valuable insights to guide future research practices, advocating for the inclusion and recognition of tāngata kāpō Māori rights in practice and research.
Implementing Aging in Place in Hong Kong: Meeting the Needs and Aspirations of Older Adults and Their Caregivers Living in Private Housing
Authors: Woo Jean , Mok Keilee , Chu Wui-Ling , Lo Regina , Ko Rina
Published: March 2024

A policy of aging in place should be accompanied by physical and social environments that support healthy aging. This article describes how a property development company in Hong Kong sought to elicit the views of older people and their caregivers towards elderly services through a market research company, using questionnaire surveys followed by focus groups. Over 80% of all participants rated healthy dietary habits and exercise, maintaining mental and spiritual health, and maintaining a generally healthy lifestyle as important. Current health concerns include long waiting times for care at public hospitals, lack of carer should dependency occur, and lack of information about what services are available in the community. Interests in services in their neighbourhood include medical care (82%), healthy lifestyle activities (66%), and home care support (55%). There was considerable interest in the provision of services that improve brain and physical function, as well as general health checks. Carers were willing to pay more for services compared with older adults themselves. The findings inform the development of pilot models of aging in place as a sustainable financial model.
TBI and Tau Loss of Function Both Affect Naïve Ethanol Sensitivity in Drosophila
Authors: Hoxha Valbona , Shrestha Gaurav , Baloch Nayab , Collevechio Sara , Laszczyk Raegan , Roman Gregg
Published: March 2024

Traumatic brain injury (TBI) is associated with alcohol abuse and higher ethanol sensitivity later in life. Currently, it is poorly understood how ethanol sensitivity changes with time after TBI and whether there are sex-dependent differences in the relationship between TBI and ethanol sensitivity. This study uses the fruit fly Drosophila melanogaster to investigate how TBI affects alcohol sensitivity and whether the effects are sex-specific. Our results indicate that flies have a significantly higher sensitivity to the intoxicating levels of ethanol during the acute phase post-TBI, regardless of sex. The increased ethanol sensitivity decreases as time progresses; however, females take longer than males to recover from the heightened ethanol sensitivity. Dietary restriction does not improve the negative effects of alcohol post-TBI. We found that tau mutant flies exhibit a similar ethanol sensitivity to TBI flies. However, TBI increased the ethanol sensitivity of dtauKO mutants, suggesting that TBI and dtau loss of function have additive effects on ethanol sensitivity.
Morpho-Physiochemical Indices and Transcriptome Analysis Reveal the Role of Glucosinolate and Erucic Acid in Response to Drought Stress during Seed Germination of Rapeseed
Authors: Ai Xueying , El-Badri Ali Mahmoud , Batool Maria , Lou Hongxiang , Gao Gengdong , Bai Chenyang , Wang Zongkai , Jiang Chunji , Zhao Xinhua , Wang Bo , Kuai Jie , Xu Zhenghua , Wang Jing , King Graham John , Yu Haiqiu , Zhou Guangsheng , Fu Tingdong
Published: March 2024

The global expansion of rapeseed seed quality has been focused on maintaining glucosinolate (GSL) and erucic acid (EA) contents. However, the influence of seed GSL and EA contents on the germination process under drought stress remains poorly understood. Herein, 114 rapeseed accessions were divided into four groups based on GSL and EA contents to investigate their performance during seed imbibition under drought stress. Our results revealed significant variations in seed germination-related traits, particularly with higher GSL and EA, which exhibited higher germination % (G%) and lower mean germination time (MGT) under drought stress conditions. Moreover, osmoregulation, enzymatic system and hormonal regulation were improved in high GSL and high EA (HGHE) versus low GSL and low EA (LGLE) seeds, indicating the essential protective role of GSL and EA during the germination process in response to drought stress. The transcriptional regulation mechanism for coordinating GSL–EA-related pathways in response to drought stress during seed imbibition was found to involve the differential expression of sugar metabolism-, antioxidant-, and hormone-related genes with higher enrichment in HGHE compared to LGLE seeds. GO enrichment analysis showed higher variations in transcription regulator activity and DNA-binding transcription factors, as well as ATP and microtubule motor activity in GSL–EA-related pathways. Furthermore, KEGG analysis identified cellular processes, environmental information processing, and metabolism categories, with varied gene participation between GSL, EA and GSL–EA-related pathways. For further clarification, QY7 (LGLE) seeds were primed with different concentrations of GSL and EA under drought stress conditions. The results showed that 200 μmol/L of GSL and 400 μmol/L of EA significantly improved G%, MGT, and seedling fresh weight, besides regulating stress and fatty acid responsive genes during the seed germination process under drought stress conditions. Conclusively, exogenous application of GSL and EA is considered a promising method for enhancing the drought tolerance of LGLE seeds. Furthermore, the current investigation could provide a theoretical basis of GSL and EA roles and their underlying mechanisms in stress tolerance during the germination process.
Evolution and Function of the Notch Signaling Pathway: An Invertebrate Perspective
Authors: Lv Yan , Pang Xuan , Cao Zhonghong , Song Changping , Liu Baohua , Wu Weiwei , Pang Qiuxiang
Published: March 2024

The highly conserved Notch signaling pathway affects embryonic development, neurogenesis, homeostasis, tissue repair, immunity, and numerous other essential processes. Although previous studies have demonstrated the location and function of the core components of Notch signaling in various animal phyla, a more comprehensive summary of the Notch core components in lower organisms is still required. In this review, we objectively summarize the molecular features of the Notch signaling pathway constituents, their current expression profiles, and their functions in invertebrates, with emphasis on their effects on neurogenesis and regeneration. We also analyze the evolution and other facets of Notch signaling and hope that the contents of this review will be useful to interested researchers.
Antibacterial Properties of Rose Bengal Conjugated to Hyaluronic Acid
Authors: Atrash Melad , Hovor Iryna , Gurianov Yanna , Barel Margarita , Semenova Olga , Brider Tamara , Nisnevitch Marina , Nakonechny Faina
Published: March 2024

Dental diseases, including conditions affecting oral structures, have become more common due to unhealthy lifestyle choices. Traditional antibiotic treatments face challenges related to the development of antibiotic resistance in bacteria. Photodynamic antibacterial chemotherapy is emerging as a promising alternative using photosensitizers to generate reactive oxygen species upon exposure to light. This article examines the photosensitizer Rose Bengal (RB) immobilized in hyaluronic acid (HA) for prolonged antibacterial action. The RB-HA conjugate demonstrated a molar ratio of approximately three RB residues to each of the ten units of HA. RB-HA exhibited a high singlet oxygen quantum yield (ΔΦ = 0.90), suggesting its efficacy in photodynamic treatment. A photostability analysis revealed slower photobleaching of RB-HA, which is essential for prolonged application. Under visible light and ultrasonic treatment, RB-HA exhibited effective antibacterial activity against Gram-positive S. aureus and Gram-negative E. coli bacteria for at least 80 days. The gradual release of RB ensured sustained bactericidal concentration. The study establishes RB-HA as a promising candidate for antimicrobial photodynamic and sonodynamic therapy in dental and other medical fields, providing enhanced stability and prolonged antibacterial efficacy.
BMP2–ERK–ATF4 Axis-Based 6-methoxybenzofuran Compound I-9 Acts as Candidate Drug for Bone Formation and Anti-Osteoporosis
Authors: Zhou Ziying , Zhao Chenxi , Li Siyan , Han Xiaoyang , Zhu Jiangyi , Xue Situ , Li Zhuorong
Published: March 2024

As the global population ages, the number of patients with osteoporosis is rapidly rising. The existing first-line clinical drugs are bone resorption inhibitors that have difficulty restoring the bone mass of elderly patients to the safe range. The range and period of use of existing peptides and monoclonal antibodies are limited, and small-molecule bone formation–promoting drugs are urgently required. We established an I-9 synthesis route with high yield, simple operation, and low cost that was suitable for future large-scale production. I-9 administration promoted bone formation and increased bone mass in mice with low bone mass in an aged C57 mouse model. Our findings revealed a hitherto undescribed pathway involving the BMP2–ERK–ATF4 axis that promotes osteoblast differentiation; I-9 has favorable biosafety in mice. This study systematically investigated the efficacy, safety, and mechanism of I-9 for treating osteoporosis and positions this drug for preclinical research in the future. Thus, this study has promoted the development of small-molecule bone-promoting drugs.
Targeting Sphingosine-1-Phosphate Signaling in Breast Cancer
Authors: Nagahashi Masayuki , Miyoshi Yasuo
Published: March 2024

In recent years, newly emerging therapies, such as immune checkpoint inhibitors and antibody-drug conjugates, have further improved outcomes for breast cancer patients. However, recurrent and metastatic breast cancer often eventually develops resistance to these drugs, and cure is still rare. As such, the development of new therapies for refractory breast cancer that differ from conventional mechanisms of action is necessary. Sphingosine-1-phosphate (S1P) is a key molecule with a variety of bioactive activities, including involvement in cancer cell proliferation, invasion, and metastasis. S1P also contributes to the formation of the cancer microenvironment by inducing surrounding vascular- and lymph-angiogenesis and regulating the immune system. In this article, we outline the basic mechanism of action of S1P, summarize previous findings on the function of S1P in cancer cells and the cancer microenvironment, and discuss the clinical significance of S1P in breast cancer and the therapeutic potential of targeting S1P signaling.
Real-World Data from the Use of Ranolazine in Patients with Stable Angina Pectoris: The RANGER Study
Authors: Olympios Christoforos , Stafylas Panagiotis , Dermitzakis Alkiviadis , Efthimiadis Ioannis , Gardikiotis Alexandros , Kakouros Stavros , Lampropoulos Stylianos , Barbetseas John , Sourgounis Angelos ,   on behalf of RANGER Investigators
Published: March 2024

Background: Although ranolazine has been available for years as a second-line treatment to reduce angina attacks in patients with stable angina pectoris, real-world data on the effectiveness, tolerability, and safety of ranolazine are limited. Methods: A non-interventional, prospective study was conducted to assess the effectiveness and safety of ranolazine. Patients eligible for enrolment had a baseline assessment between one and fourteen days after initiating ranolazine for the first time and a follow-up visit three months later. The primary endpoints comprised the weekly frequency of angina attacks, total adverse events, and ranolazine discontinuation rate. The secondary endpoints included the use of short-acting nitrates, changes on the Canadian Cardiovascular Society (CCS) angina classification score and quality of life scale score (QoL). Results: In total, 1101 patients were enrolled at 214 sites. Mean weekly angina attacks were reduced from 3.6 ± 2.9 to 0.4 ± 0.9 (p < 0.0001) and the mean weekly consumption of short-acting nitrates decreased by 1.7 ± 2.2 (p < 0.0001). CCS class and QoL were also improved (p < 0.0001). Adverse events were reported by 11 (1%) patients in total, while 2 of them (0.2%) were characterised as serious. Treatment was discontinued for various reasons in 23 patients (2.1%) after the follow-up period. Ranolazine treatment was equally effective in all subgroups tested, with larger benefits observed in patients with more frequent angina and CCS angina class III and IV. Up-titration of ranolazine during the study improved the outcomes. Conclusions: Ranolazine was well tolerated and effectively reduced angina attacks, with simultaneous improvement of the CCS class and QoL score in patients with stable angina.
Sociodemographic and Medical Characteristics of Women Applied for Emergency Contraception—A Retrospective Observational Study
Authors: Tóth Richárd , Lőczi Lotti , Török Marianna , Keszthelyi Attila , Leipold Gergő , Ács Nándor , Várbíró Szabolcs , Keszthelyi Márton , Lintner Balázs
Published: March 2024

Background: Lifestyle factors significantly impact overall health. Our aim was to assess reproductive health awareness among patients who applied for emergency contraceptive pills. Methods: This present retrospective observational study between July 2021 and September 2021 is embedded in the MEEC (Motivation and Epidemiology of Emergency Contraceptive Pill) based on the study cohort of a Hungarian data bank containing follow-up data of 447 women who applied for EC telemedicine consultation. Collected data: age, history of previous pregnancy, lifestyle factors like smoking, alcohol consumption, sexual characteristics: partner consistency and protection during intercourse, cervical cancer screening within the past 2 years, previous HPV screening, and the preference for future contraceptive methods. The investigation also compiled accurate data on intercourse (elapsed time to request a medical consultation). Lifestyle factors were scored. Results: The more health-conscious patients were quicker to report for a post-event pill. Earlier pregnancies and older age were associated with greater reproductive health awareness. Conclusions: Reproductive health awareness is increased by previous pregnancies and older age. More health-conscious women consult a doctor earlier, which can reduce the chance of various health damage. Our study emphasizes the significance of lifestyle factor influence on reproductive health decisions.
Clinical Characteristics and Incidence of Hemorrhagic Complications in Patients Taking Factor Xa Inhibitors in Spain: A Long-Term Observational Study
Authors: Escobar Carlos , Palacios Beatriz , Villarreal Miriam , Gutiérrez Martín , Capel Margarita , Hernández Ignacio , García María , Lledó Laura , Arenillas Juan F.
Published: March 2024

Objective. To analyze the clinical characteristics of patients taking Factor Xa inhibitors (FXai), either direct FXai or enoxaparin (only in active cancer patients), and to estimate the incidence of and risk factors for major bleeding during FXai use. Methods. A retrospective cohort study, which included secondary data from computerized health records of primary care centers and hospitals in seven Spanish Autonomous Communities. Results. 9374 patients were analyzed, with 8972 taking direct FXai and 402 enoxaparin. At baseline, the mean age (SD) was 71.8 (9.4) years, 56.0% were women, 76.3% had hypertension, 33.6% had type 2 diabetes, and 25.5% had heart failure. The most common indication for FXai use was atrial fibrillation (72.3%), followed by venous thromboembolism (22.2%) and non-mechanical cardiac–valve replacement (5.6%). At the end of the follow-up period, the incidence rates of major bleeding overall, gastrointestinal, and intracranial were 10.2, 9.0, and 0.8 per 100 person-years, respectively. The total incidence of fatal major bleeding was 0.5 per 100 person-years. Incidence rates of all bleedings progressively decreased over time, with 62.5% of the first events occurring in the initial three months and reaching 76.8% within six months following initiation of treatment. Only 4.8% of the 1st major bleedings led to death, 2.3% in the case of major gastrointestinal bleeding, and 30.8% after an intracranial bleeding. 65.9% of patients discontinued anticoagulation after experiencing major bleeding. Conclusions. In Spain, patients taking FXai were old and had many comorbidities. Despite incidence rates of major bleeding were high, incidence rates of intracranial and fatal bleedings were low, but more efforts are required due to their relevant clinical impact.
The Longitudinal Association between Multiple Frailty Criteria and Depressive Symptomatology Five Years Later in Older Adults: A Decision Tree Modelling Approach
Authors: Nascimento Marcelo de Maio , Marques Adilson , Gouveia Élvio R. , Marconcin Priscila , Peralta Miguel , Ihle Andreas
Published: March 2024

Background: To examine the longitudinal association between frailty criteria and depression (DEP) in a large sample of older Europeans using decision tree models, and to examine complex relationships between frailty criteria and DEP symptomatology. Methods: Data come from waves six and eight of the Population Survey of Health, Ageing and Retirement in Europe. DEP was assessed using the EURO-D scale (wave 8) and frailty (wave 6). We included 27,122 people (56.9% women), aged 50 or over. Results: Women indicated a higher rate of DEP (29.0%), as well as a higher prevalence of pre-frailty (21.6%) and frailty (10.8%) than men. For both sexes, fatigue, weight loss, and slowness indicated an increased chance of DEP 5 years later. MPA (moderate physical activity) and grip strength were considered longitudinally protective factors for DEP. The highest prevalence of DEP symptomatology 5 years later was 50.3%, pointing to those with fatigue and slowness. Among women, the highest incidence of DEP was 66.8%, identified through fatigue, slowness, and low MPA. Conclusions: Strategies to reduce frailty and DEP in older European adults may include the creation of policies that encourage the promotion of physical capacity to reach MPA levels, as well as an improvement in muscular strength.
Surgical Management of Chiari 1.5 in Children: A Truly Different Disease?
Authors: Vetrano Ignazio G. , Barbotti Arianna , Galbiati Tommaso Francesco , Mariani Sabrina , Erbetta Alessandra , Chiapparini Luisa , Saletti Veronica , Valentini Laura G.
Published: March 2024

Background: In patients with Chiari 1.5 malformation (CM1.5), a more aggressive disease course and an increased association with craniovertebral junction (CVJ) anomalies has been suggested. The best management of this subgroup of patients is not clearly defined, also due to the lack of specific series elucidating this anomaly’s peculiar characteristics. Methods: We evaluated a series of 33 patients (25 females, 8 males; mean age at surgery: 13 years) fulfilling the criteria for Chiari 1.5 diagnosis who underwent posterior fossa decompression and duraplasty (PFDD) between 2006 and 2021. Results: Headache was present in all children, five presented central apnea, five had dysphagia, and three had rhinolalia. Syringomyelia was present in 19 (58%) children. Twenty patients (61%) showed various CVJ anomalies, but only one child presented instability requiring arthrodesis. The mean tonsil displacement below the foramen magnum was 19.9 mm (range: 12–30), without significant correlation with the severity of symptoms. Syringomyelia recurred or was unchanged in three patients, and one needed C1–C2 fixation. The headache disappeared in 28 children (84%). Arachnoid opening and tonsil coagulation or resection was necessary for 19 children (58%). Conclusions: In our pediatric CM series, the need for tonsil resection or coagulation was higher in CM1.5 children due to a more severe crowding.
The Complexity of Skeletal Transverse Dimension: From Diagnosis, Management, and Treatment Strategies to the Application of Collaborative Cross (CC) Mouse Model
Authors: Watted Nezar , Lone Iqbal M. , Midlej Kareem , Zohud Osayd , Awadi Obaida , Masarwa Samir , Watted Ali , Paddenberg Eva , Krohn Sebastian , Kirschneck Christian , Proff Peter , Iraqi Fuad A.
Published: March 2024

This study investigates the significance of skeletal transverse dimension (STD) in orthodontic therapy and its impact on occlusal relationships. The primary goal is to enhance understanding and promote the integration of transverse skeletal diagnostics into routine orthodontic assessments. To achieve this aim, the study employs a comprehensive approach, utilizing model analysis, clinical assessments, radiographic measurements, and occlusograms. The initial step involves a meticulous assessment of deficiencies in the maxilla, mainly focusing on transverse dimension issues. Various successful diagnostic methods are employed to ascertain the type and presence of these deficiencies. Furthermore, the study compares surgically assisted maxillary expansion (SARME) and orthopedic maxillary expansion (OME) in addressing skeletal transverse issues. Stability assessments and efficacy analyses are conducted to provide valuable insights into the superiority of SARME over OME. The findings reveal that proper evaluation of STD is crucial in orthodontic diagnosis, as overlooking transverse dimension issues can lead to complications such as increased masticatory muscle activity, occlusal interferences, and an elevated risk of gingival recession. Surgically assisted maxillary expansion emerges as a more stable solution than orthopedic methods. In conclusion, incorporating skeletal transverse diagnostics into routine orthodontic assessments is imperative for achieving optimal occlusal relationships and minimizing negative consequences on dentition, periodontium, and joints. The study emphasizes the significance of accurate three-dimensional assessments and recommends the consideration of SARME over OME for addressing skeletal transverse deficiencies. Finally, the Collaborative Cross (CC) mouse model is also a novel mouse model for studying complex traits. Exploring the Collaborative Cross mouse model opens avenues for future research, promising further insights into transverse skeletal issues in orthodontics.
An Improved Reeds–Shepp and Distributed Auction Algorithm for Task Allocation in Multi-AUV System with Both Specific Positional and Directional Requirements
Authors: Li Hongfei , Zhu Daqi , Chen Mingzhi , Wang Tong , Zhu Hongxiu
Published: March 2024

Task assignment is of paramount importance in multi-AUV systems, particularly in applications such as bridge inspection where task execution is direction-specific. In such scenarios, the underactuation of AUVs is a critical factor that cannot be ignored. Therefore, it is essential to consider the AUV’s kinematic model comprehensively to ensure minimal energy consumption during task execution. In this paper, we introduce an improved Reeds–Shepp algorithm in conjunction with a distributed auction approach. We treat AUVs as car-like models in our approach, paying meticulous attention to their operational characteristics during path planning. Importantly, we effectively utilize their backward driving capabilities. Our analysis reveals that this model successfully fulfills the directional requirements of detection tasks. Furthermore, the distributed auction approach optimizes the overall task distribution in the multi-AUV system. We support our method with simulation results that underscore its effectiveness.
Robust Fish Recognition Using Foundation Models toward Automatic Fish Resource Management
Authors: Hasegawa Tatsuhito , Nakano Daichi
Published: March 2024

Resource management for fisheries plays a pivotal role in fostering a sustainable fisheries industry. In Japan, resource surveys rely on manual measurements by staff, incurring high costs and limitations on the number of feasible measurements. This study endeavors to revolutionize resource surveys by implementing image-recognition technology. Our methodology involves developing a system that detects individual fish regions in images and automatically identifies crucial keypoints for accurate fish length measurements. We use grounded-segment-anything (Grounded-SAM), a foundation model for fish instance segmentation. Additionally, we employ a Mask Keypoint R-CNN trained on the fish image bank (FIB), which is an original dataset of fish images, to accurately detect significant fish keypoints. Diverse fish images were gathered for evaluation experiments, demonstrating the robust capabilities of the proposed method in accurately detecting both fish regions and keypoints.
Variable Natural Frequency Damper for Minimizing Response of Offshore Wind Turbine: Effect on Dynamic Response According to Inner Water Level
Authors: Kim Dong-Ju , You Young-Suk , Sun Min-Young
Published: March 2024

Offshore wind turbines (OWTs) are exposed to cyclic loads resulting from wind, waves, and rotor rotation. These loads can induce resonance, thereby significantly increasing the amplitude of the structure and accelerating the accumulation of fatigue damage. Particularly, wave loads can induce the first mode of large turbines. While many studies have been conducted to suppress OWT vibrations due to external loads, research on variable natural frequency damper (VNFD), which control vibrations through changes in the natural frequency by adjusting the inner water level of the structure, is still in its infancy. Herein, the performance of a VNFD in controlling the vibration of monopile-type OWTs is analyzed by focusing on cyclic environmental loads. To analyze the amplitude minimization achieved using a VNFD, wave loads with the same period as that of the structure’s natural frequency were generated, and the structural response resulting from changes in the inner water level were analyzed. As a result, the peak displacement at the top of the tower decreased by 5.8% and 34% at the water depths of 20 m and 50 m, respectively. In terms of the peak intensity determined through Fast Fourier Transform of the displacement response, reductions of 33% and 65% were confirmed at the depths of 20 m and 50 m, respectively.
Application of Regularized Meshless Method with Error Estimation Technique for Water–Wave Scattering by Multiple Cylinders
Authors: Chen Kue-Hong , Kao Jeng-Hong , Hsu Yi-Hui
Published: March 2024

In this manuscript, we will apply the regularized meshless method, coupled with an error estimation technique, to tackle the challenge of modeling oblique incident waves interacting with multiple cylinders. Given the impracticality of obtaining an exact solution in many real engineering problems, we introduce an error estimation technique designed to achieve reliable solutions. This technique excels in providing dependable solutions that closely approximate analytical solutions. An additional advantage is its capacity to identify the optimal number of points for both source and collocating points, thereby enhancing computational efficiency. The validity of the proposed method will be demonstrated through three numerical cases, presenting results that exhibit substantial agreement.