bluuebunny/crossref_metadata_2025 huggingface dataset:
Tree Cover Species Modify the Diversity of Rhizosphere-Associated Microorganisms in Nothofagus obliqua (Mirb.) Oerst Temperate Forests in South-Central Chile
Authors: Almonacid-Muñoz Leonardo , Herrera Hector , Fuentes-Ramírez Andres , Vargas-Gaete Rodrigo , Larama Giovanni , Jara Ronald , Fernández-Urrutia Camila , da Silva Valadares Rafael Borges
Published: May 2022

Chilean native forests have been subjected to several types of disturbances, with one of them being the replacement by exotic species. Pinus radiata D. Don is a widespread exotic tree that forms extensive plantations in southern Chile. It covers extended areas, affecting the landscape, biodiversity, and ecosystem services associated with native forest ecosystems. Although advances in assessing the impact of exotic plant species have been conducted, few studies have focused on the alteration of soil microorganisms. This study aimed to characterize the rhizosphere bacterial and fungal communities associated with the tree species Nothofagus obliqua inside a native forest stand and within a P. radiata plantation growing nearby. We used a 16S rRNA gene and ITS region metabarcoding approach. Using bioinformatics, diversity indices, relative abundance, preferential taxa, and predicted functions and guilds were estimated. The β-diversity analysis showed that both factors, the type of soil (rhizosphere or bulk soil) and the type of site (native forest or P. radiata plantation), were significant, with the site explaining most of the variation among bacterial and fungal communities. Proteobacteria and Basidiomycota were the most abundant bacterial and fungal phyla in both types of soil and sites. Similarly, bacteria showed similar abundant taxa at the family level, independent of the soil type or the site. The main fungal taxa associated with native forests were Tricholomataceae and Cantharellales, whereas in P. radiata plantations, Russulaceae and Hyaloscyphaceae were the most abundant families. The main bacteria functional groups were chemoheterotrophy and aerobic chemoheterotrophy, without significant differences between the type of soil or sites. Overall, these results demonstrate that the composition and diversity of bacterial and fungal communities associated with native N. obliqua forest are influenced by the surrounding forest, and mainly depend on the site’s characteristics, such as the lignin-rich wood source. These results improve our understanding of the impact of native forest replacement on soil microbial communities, which can alter microbial-related soil ecosystem services.
Creep Properties of Densified Wood in Bending
Authors: Han Lei , Kutnar Andreja , Couceiro José , Sandberg Dick
Published: May 2022

Thermo-hydro-mechanical (THM)-densified timber is rarely used in construction, although its mechanical properties are in many cases excellent. The main reason for its rare use is set-recovery, which reduces the degree of densification over time so that the mechanical properties deteriorate. Our knowledge of the long-term creep of densified timber is insufficient and a full understanding of its long-term behaviour is still lacking. The purpose of this study was to examine the behaviour under long-term loading of Scots pine sapwood densified in an open system at 170–200 °C. The influence of the THM densification process on the creep properties was studied on (1) unmodified specimens, (2) THM-densified specimens, (3) THM-densified specimens that had been further thermally treated, and (4) low-molecular-weight phenol-formaldehyde resin-impregnated and THM-densified specimens. All specimens were loaded at 20 ± 2 °C and 65 ± 5% relative humidity for 14 days under 3-point bending at 35% of the short-term ultimate load, and the bending deformation was registered. The THM densification doubled the density, causing a significant increase in the modulus of rupture but no change in the modulus of elasticity, and reduced the equilibrium moisture content and creep compliance. Post-thermal modification and resin impregnation improved the dimensional stability and further reduced the creep compliance in bending. The results demonstrate that THM densification combined with resin-impregnation or thermal modification reduces the creep of Scots pine timber under a long-term bending load in a constant climate.
Academic Success, Emotional Intelligence, Well-Being and Resilience of First-Year Forestry Students
Authors: Năstasă Laura Elena , Cocoradă Elena , Vorovencii Iosif , Curtu Alexandru Lucian
Published: May 2022

Academic success is conceptualized as a multifactor model, achievement and persistence after the first year of study being considered the main learning outcomes. In this area, the findings are inconsistent, depending on the academic context, individual characteristics and diversity of psychological measures. Here, we conducted two related correlational studies to analyze variables of the emotional domain, as emotional intelligence (2017–2019), emotions, well-being and resilience (2018–2020), dropout intention, perceived barriers to completion at the beginning of first years of study in forestry, academic performance and real dropout at the end of the first year of study and several socio-demographic variables. The two studies focused on undergraduate students and included 367 and 227 participants, respectively. Forestry students with higher academic performance report higher accomplishment and engagement and feel weak negative emotions concerning aversive academic assessment compared with students with lower academic performance and students that abandoned their studies. Female students and students with full ECTS load at the end of the first year use their emotions more effectively, have reported lower dropout intention and lower perception of barriers to completion of study, and have higher GPA admission and expected GPA. Performance at the end of the first year is explained by GPA admission, relationships with high school teachers, expected GPA, gender, and academic resilience. Dropout intention is explained by barriers to completion of studies, general negative emotions and negative affect related to threatened assessment situations, and managing others’ emotions. Our findings may help develop intervention measures at the individual and organizational level.
Data-Driven Optimization of Forestry and Wood Procurement toward Carbon-Neutral Logistics of Forest Industry
Authors: Palander Teijo , Vesa Lauri
Published: May 2022

Investments toward a carbon-neutral forest industry will change forestry and wood procurement in Northern Finland. The changing market situation requires data-driven DSSs for the strategic management of logistics. Using this software, logistics were described by a continuing wood flow model and optimized by a dynamic method. Three logistics scenarios described wood flows in the present and in the future. The optimization minimized the economic and environmental costs, which decreased by 4.9%. However, synchronized multimodal transportation costs increased by 23.3%. Therefore, maximum logistics efficiency necessitates increases in railway transport capacity. The change would also decrease CO2 emission costs. Under scenario-specific circumstances, logistics operations could be focused on four profitable regions, increasing market shares at municipalities. To guarantee environmental sustainability of these municipalities, optimization of timber markets between forest owners and forest industry must be developed further by driving data from the EU’s emission allowance price compensation mechanism to the optimization process.
Designing a Waste-Based Culture Medium for the Production of Plant Growth Promoting Microorganisms Based on Cladodes Juice from Opuntia ficus-indica Pruning
Authors: Magarelli Rosaria Alessandra , Trupo Mario , Ambrico Alfredo , Larocca Vincenzo , Martino Maria , Palazzo Salvatore , Balducchi Roberto , Joutsjoki Vesa , Pihlanto Anne , Bevivino Annamaria
Published: May 2022

The production of beneficial microorganisms is the first step to obtain a commercial-based product for application in agriculture. In this study, prickly pear (Opuntia ficus-indica) pruning waste was evaluated as a raw material for the production of large amounts of Plant Growth Promoting Microorganisms (PGPMs) reducing the number of generated wastes. Specifically, five PGPMs constituting a synthetic microbial consortium with complementing plant growth-promoting traits were grown on a laboratory scale and, subsequently, on a pilot scale using a 21-L bioreactor. Primarily, the physical-chemical characterization of the culture medium obtained from the juice of Opuntia cladodes was carried out, revealing the presence of sugars and organic acids with different molar ratios. Compared to conventional media, the waste medium did not show significant differences in bacterial growth efficiency. Instead, the survival rates of the bacteria grown in cladodes juice media, after air-drying on zeolite or freeze-drying, were significantly higher than those observed when they were grown in conventional media. The present work is the first conducted on a pilot-scale that maximizes the production of PGPMs in submerged fermentation using cladodes juice from Opuntia, reducing both economic and environmental impacts associated with the generation of wastes.
QoE Models for Adaptive Streaming: A Comprehensive Evaluation
Authors: Nguyen Duc , Pham Ngoc Nam , Thang Truong Cong
Published: May 2022

Adaptive streaming has become a key technology for various multimedia services, such as online learning, mobile streaming, Internet TV, etc. However, because of throughput fluctuations, video quality may be dramatically varying during a streaming session. In addition, stalling events may occur when segments do not reach the user device before their playback deadlines. It is well-known that quality variations and stalling events cause negative impacts on Quality of Experience (QoE). Therefore, a main challenge in adaptive streaming is how to evaluate the QoE of streaming sessions taking into account the influences of these factors. Thus far, many models have been proposed to tackle this issue. In addition, a lot of QoE databases have been publicly available. However, there have been no extensive evaluations of existing models using various databases. To fill this gap, in this study, we conduct an extensive evaluation of thirteen models on twelve databases with different characteristics of viewing devices, codecs, and session durations. Through experiment results, important findings are provided with regard to QoE prediction of streaming sessions. In addition, some suggestions on the effective employment of QoE models are presented. The findings and suggestions are expected to be useful for researchers and service providers to make QoE assessments and improvements of streaming solutions in adaptive streaming.
Investigation of Spontaneous Combustion Zones and Index Gas Prediction System in Goaf of “Isolated Island” Working Face
Authors: Chai Jiamei
Published: May 2022

Studies show that accurate division of spontaneous combustion (SC) zones in the goaf and the determination of the prediction system of the SC index are of great significance to prevent spontaneous and unwanted combustions in the goaf. Aiming at resolving the problem of coal SC in the goaf of an “isolated-island” fully mechanized caving face, a multiphysics model coupled with gas flow field and gas concentration field was established in the present study. Taking the 8824 working face of Nanzhuang coal mine as the research object and the oxygen concentration as the division index, coal SC was simulated in the goaf. The obtained results show that the ranges of heat dissipation zone, oxidation zone, and the asphyxia zone on the air inlet side are around 0–107 m, 107–239 m, and beyond 239 m, respectively. Moreover, the ranges of the three zones on the return air side are 0–13 m, 13–189 m, and beyond 189 m, respectively. The ranges of the three zones in the middle of goaf are 0–52 m, 52–213 m, and beyond 213 m, respectively. The performed analyses demonstrate that the obtained simulation results are consistent with the experimental data. Meanwhile, the coal programmed temperature rise experiment was carried out to improve the prediction index gas system of SC. It was found that CO and C2H4 can be used as early warning indices of SC in the goaf, while C2H6, C3H8, and C2H4/C2H6 are auxiliary indices to master the coal SC.
The Beneficial Effects of Two Polysaccharide Fractions from Sargassum fusiform against Diabetes Mellitus Accompanied by Dyslipidemia in Rats and Their Underlying Mechanisms
Authors: Jia Rui-Bo , Wu Juan , Luo Donghui , Lin Lianzhu , Chen Chong , Xiao Chuqiao , Zhao Mouming
Published: May 2022

The current study aimed to assess the anti-diabetic effects and potential mechanisms of two Sargassum fusiform polysaccharide fractions (SFPs, named SFP-1 and SFP-2). The carbohydrate-loading experiment revealed that SFP-2 could control postprandial hyperglycemia by inhibiting the activity of digestive enzymes in rats. The analysis of diabetic symptoms and serum profiles indicated that SFPs could mitigate diabetes accompanied by dyslipidemia, and SFP-2 showed better regulatory effects on body weight, food intake and the levels of total cholesterol (TC), triglycerides (TG), low density lipoprotein-cholesterol (LDL-C) and free fatty acid (FFA) in diabetic rats. Intestinal bacterial analysis showed that SFP treatment could reshape the gut flora of diabetic rats, and SFP-2 possessed a greater regulatory effect on the growth of Lactobacillus and Blautia than SFP-1. RT-qPCR analysis revealed that SFPs could regulate the genes involved in the absorption and utilization of blood glucose, hepatic glucose production and lipid metabolism, and the effects of SFP-2 on the relative expressions of Protein kinase B (Akt), Glucose-6-phosphatase (G-6-Pase), Glucose transporter 2 (GLUT2), AMP-activated protein kinase-α (AMPKα), Peroxisome proliferator-activated receptor γ (PPARγ) and Cholesterol 7-alpha hydroxylase (CYP7A1) were greater than SFP-1. All above results indicated that SFPs could be exploited as functional foods or pharmaceutical supplements for the treatment of diabetes and its complications.
Valorisation of Bovine Sweet Whey and Sunflower Press Cake Blend through Controlled Fermentation as Platform for Innovative Food Materials
Authors: Mangieri Nicola , Ambrosini Davide , Baroffio Stefano , Vigentini Ileana , Foschino Roberto , De Noni Ivano
Published: May 2022

The current environmental challenge is pushing food systems towards more sustainable models of production that require reorganizing of processes by re-using side products still containing nutrients. This work aimed at valorising a mix of bovine sweet whey and sunflower press cake, through targeted fermentation. After preliminary screening based on growth rate, final pH, lactose/galactose assimilation, phytase activity, six Lactic Acid Bacteria strains (Lacticaseibacillus casei, L. paracasei (2), Lactococcus lactis, Lentilactobacillus parakefiri and Leuconostoc pseudomesenteroides) and three yeasts (Kluyveromyces lactis, K. marxianus and Torulaspora delbrueckii) were co-cultivated in pairs in microcosms (1-part ground press cake: 4-parts whey). All tested microorganisms were able to grow and acidify the blend: the LAB counts increased during the incubation (26 °C for 48 h) of +2.80 log CFU/g, whereas yeasts counts were of +1.98 log CFU/g, with significant differences among the different associations (p < 0.01). Mould counts were always <3 log CFU/g. Interestingly, the bacterial contaminants count significantly varied in samples with different pairs of strains (p < 0.001). Acidification level, acetic acid and ethanol contents were the limiting factors affecting the growth of spoilage micro-organisms. Best performances were attained in microcosms inoculated with L. lactis or L. paracasei and K. lactis or K. marxianus.
Stevia (Stevia rebaudiana) Improves Carotenoid Content in Eggs When Fed to Laying Hens
Authors: Pirgozliev Vasil Radoslavov , Whiting Isobel Margaret , Kljak Kristina , Mansbridge Stephen Charles , Atanasov Atanas Georgiev , Rose Stephen Paul , Enchev Stanimir Bojidarov
Published: May 2022

Stevia rebaudiana Bertoni is a shrub with leaves that have a high concentration of carotenoids such as lutein and zeaxanthin. Egg yolks are a bioavailable source of lutein and zeaxanthin. The consumption of these carotenoids has been linked with improved human health. To investigate the impact of dried stevia leaves at 0%, 1% and 2% on the quality variables, the chemical composition and antioxidant content of eggs, the experiment involved 90 Hy-Line Brown laying hens, housed in 30 enriched layer cages, in groups of three from 22 to 26 weeks of age. The impact on the internal qualities of stored eggs was also examined. Yolks from hens fed stevia had an enriched color compared with the controls. At the end of the experiment, the whole egg, without shell, of birds fed 2% stevia had a higher total carotenoid content (p < 0.001) compared with birds fed 1% and 0% stevia, i.e., 5.16 (µg/g), 4.23 (µg/g) and 2.96 (µg/g), respectively. Storage reduced albumen height and increased albumen pH (p < 0.001). Stevia supplementation did not interact (p > 0.05) with storage time among the egg quality variables. Consuming eggs from hens fed stevia may increase carotenoids in human diet.
Commercial Baby Foods Aimed at Children up to 36 Months: Are They a Matter of Concern?
Authors: Santos Mariana , Matias Filipa , Loureiro Isabel , Rito Ana Isabel , Castanheira Isabel , Bento Alexandra , Assunção Ricardo
Published: May 2022

Proper nutrition in infancy and early childhood is crucial to ensuring optimal child development, growth, and better health outcomes later in life. The nutrient profile model proposed by WHO/Europe aims to assess the nutritional quality and promotional/marketing aspects of commercial baby foods aimed at children up to 36 months. We used commercial data from 191 baby foods collected between March 2021 and July 2021, from eight supermarket chains in the Lisbon Metropolitan area. According to the model specifications and the NOVA classification system, we assessed the nutritional quality and promotion aspects and the degree of processing, respectively. The presence of at least one sugar-contributing ingredient was found in 34.0% of the products; 13.9% of products listed sugars and 15.0% listed fruit juices or concentrates as an ingredient. The claim “No added sugar” was present in 69.6% of products. Only 35.1% of products comply with all the nutritional requirements of the model. Concerning processing classification, 61.8% of products were ultra-processed, and about 57.0% were indicated for children < 12 months. These findings reinforce the importance of implementing measures to ensure that commercial foods for infants are marketed appropriately and to promote foods with a lower degree of processing.
Recent Advances in Analytical Methods for Determination of Polyphenols in Tea: A Comprehensive Review
Authors: Sun Mu-Fang , Jiang Chang-Ling , Kong Ya-Shuai , Luo Jin-Lei , Yin Peng , Guo Gui-Yi
Published: May 2022

Polyphenols, the most abundant components in tea, determine the quality and health function of tea. The analysis of polyphenols in tea is a topic of increasing interest. However, the complexity of the tea matrix, the wide variety of teas, and the difference in determination purposes puts forward higher requirements for the detection of tea polyphenols. Many efforts have been made to provide a highly sensitive and selective analytical method for the determination and characterization of tea polyphenols. In order to provide new insight for the further development of polyphenols in tea, in the present review we summarize the recent literature for the detection of tea polyphenols from the perspectives of determining total polyphenols and individual polyphenols in tea. There are a variety of methods for the analysis of total tea polyphenols, which range from the traditional titration method, to the widely used spectrophotometry based on the color reaction of Folin–Ciocalteu, and then to the current electrochemical sensor for rapid on-site detection. Additionally, the application of improved liquid chromatography (LC) and high-resolution mass spectrometry (HRMS) were emphasized for the simultaneous determination of multiple polyphenols and the identification of novel polyphenols. Finally, a brief outline of future development trends are discussed.
Primary Obsessive Slowness: A Complex Presentation and Treatment Difficulties
Authors: Reddy Balaswamy , de Filippis Renato , Nocera Alessandra , Das Soumitra
Published: May 2022

Obsessive slowness is described as a complex and disabling clinical syndrome that causes extreme slowness in performing tasks, with potential personal and functional impairment. It is a rare condition with a challenging differential diagnosis with obsessive-compulsive disorders, mental retardation and catatonia, and its existence as an independent syndrome is still debated by authors and not included by classification systems. Therefore, its treatment management is not well-defined and it still represents a clinical challenge for clinicians. Currently, the main proposal is a mix of antidepressant, antipsychotic, psychoeducation, psychotherapy and biological non-pharmacological interventions. Hereby, we describe a case of an 18-year-old male patient who presented debilitating slowness and severe impairment. Managing his treatment was particularly challenging for clinicians and was ultimately improved with escitalopram 30 mg/day combined with memantine 10 mg/day and amisulpride 400 mg/day.
Investigating the Mechanical Property and Enhanced Mechanism of Modified Pisha Sandstone Geopolymer via Ion Exchange Solidification
Authors: Li Changming , Song Lisha , Cao Yali , Zhao Shunbo , Liu Hui , Yang Chen , Cheng Haifeng , Jia Dongyang
Published: May 2022

The Yellow River has the highest sediment concentration in the world, and the Yellow River coarse sediment mainly comes from a particular kind of argillaceous sandstone, Pisha sandstone. This paper reports an investigation of the possibility of development of low-cost engineering materials using Pisha sandstone via ion exchange modification. The effect of modifiers with different concentration on the inhibition of volume expansion and the strength enhancement of modified Pisha sandstone were studied via ion exchange solidification. The effects of the concentration of ten types of modifier solutions and curing age were considered. The hydration of the mineral components, particle surface potential and reaction products were studied, respectively, by XRD, zeta potential, TG/DTG and SEM. Expansion volume and shear strength tests were conducted to assess the volume stability and mechanical property of modified Pisha sandstone. It showed that the expansion of Pisha sandstone was controlled and that the volume stability and shear strength were improved via ion exchange modification. The results of XRD, TG/DTG and SEM showed that the spacing of the crystal layers of the Pisha sandstone clay mineral and the mass lost had decreased significantly. When the concentration of the modifier was 0.05 mol/L, the volume reduced by 54.55% maximum and the shear strength reached the peak of 138 kPa.
High Expression of EZH2 Mediated by ncRNAs Correlates with Poor Prognosis and Tumor Immune Infiltration of Hepatocellular Carcinoma
Authors: Chen Zhitao , Lin Xin , Wan Zhenmiao , Xiao Min , Ding Chenchen , Wan Pengxia , Li Qiyong , Zheng Shusen
Published: May 2022

Background: Hepatocellular carcinoma (HCC) is the predominant form of liver cancer and is accompanied by a complex regulatory network. Increasing evidence suggests that an abnormal gene expression of EZH2 is associated with HCC progression. However, the molecular mechanism by which non-coding RNAs (ncRNAs) regulate EZH2 remains elusive. Methods: The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data were used to perform differential expression analysis and prognostic analysis. We used the Encyclopedia of RNA Interactomes (ENCORI) database to predict candidate miRNAs and lncRNAs that may bind to EZH2. Subsequently, the comprehensive analysis (including expression analysis, correlation analysis, and survival analysis) identified ncRNAs that contribute to EZH2 overexpression. Results: EZH2 was found to be upregulated in the majority of tumor types and associated with a poor prognosis. Hsa-miR-101-3p was identified as a target miRNA of EZH2. Additionally, SNHG6 and MALAT1 were identified as upstream lncRNAs of hsa-miR-101-3p. Meanwhile, correlation analysis revealed that EZH2 expression was significantly associated with the infiltration of several immune cell types in HCC. Conclusion: SNHG6 or MALAT1/hsa-miR-101-3p/EZH2 axis were identified as potential regulatory pathways in the progression of HCC.
Potential Misidentification of Natural Isomers and Mass-Analogs of Modified Nucleosides by Liquid Chromatography–Triple Quadrupole Mass Spectrometry
Authors: Lin Xiuying , Zhang Qianhui , Qin Yichao , Zhong Qisheng , Lv Daizhu , Wu Xiaopeng , Fu Pengcheng , Lin Huan
Published: May 2022

Triple quadrupole mass spectrometry coupled to liquid chromatography (LC-TQ-MS) can detect and quantify modified nucleosides present in various types of RNA, and is being used increasingly in epitranscriptomics. However, due to the low resolution of TQ-MS and the structural complexity of the many naturally modified nucleosides identified to date (>160), the discrimination of isomers and mass-analogs can be problematic and is often overlooked. This study analyzes 17 nucleoside standards by LC-TQ-MS with separation on three different analytical columns and discusses, with examples, three major causes of analyte misidentification: structural isomers, mass-analogs, and isotopic crosstalk. It is hoped that this overview and practical examples will help to strengthen the accuracy of the identification of modified nucleosides by LC-TQ-MS.
Polymerase ζ Is Involved in Mitochondrial DNA Maintenance Processes in Concert with APE1 Activity
Authors: Schreier Heike Katrin , Wiehe Rahel Stefanie , Ricchetti Miria , Wiesmüller Lisa
Published: May 2022

Mitochondrial DNA (mtDNA) damaged by reactive oxygen species (ROS) triggers so far poorly understood processes of mtDNA maintenance that are coordinated by a complex interplay among DNA repair, DNA degradation, and DNA replication. This study was designed to identify the proteins involved in mtDNA maintenance by applying a special long-range PCR, reflecting mtDNA integrity in the minor arc. A siRNA screening of literature-based candidates was performed under conditions of enforced oxidative phosphorylation revealing the functional group of polymerases and therein polymerase ζ (POLZ) as top hits. Thus, POLZ knockdown caused mtDNA accumulation, which required the activity of the base excision repair (BER) nuclease APE1, and was followed by compensatory mtDNA replication determined by the single-cell mitochondrial in situ hybridization protocol (mTRIP). Quenching reactive oxygen species (ROS) in mitochondria unveiled an additional, ROS-independent involvement of POLZ in the formation of a typical deletion in the minor arc region. Together with data demonstrating the localization of POLZ in mitochondria, we suggest that POLZ plays a significant role in mtDNA turnover, particularly under conditions of oxidative stress.
Attitude, Beliefs, and Use of Herbal Remedies by Patients in the Riyadh Region of Saudi Arabia
Authors: Alghadir Ahmad H. , Iqbal Amir , Iqbal Zaheen A.
Published: May 2022

Background: The consumption of herbal medicines (HMs) is increasing worldwide, especially in developing countries. This study attempts to investigate and evaluate the patient’s perception with regard to the security of HMs, their attitudes towards the concomitant use of HMs with modern medicines, and counseling about their use. Design: Self-administered questionnaire-based cross-sectional survey study. Setting: A self-administered structured questionnaire was administered to 200 patients who received HMs from four different government and private hospitals in the Riyadh region of Saudi Arabia, over a period of three months. Results: The response rate was 74.5%. Out of these, 76.83% of respondents reported using HMs in some form for a variety of conditions. There was no statistically significant relationship between various demographic characteristics and the use of herbs. The majority of the respondents (76.72%) reported using HMs without any professional supervision. This exposes them to the risk of harmful side effects and drug interactions. Conclusions: Physicians and pharmacists should work to provide evidence-based information about HMs to patients about effectiveness and side effects and be vigilant while writing prescriptions and dispensing drugs to them. Patient counseling and education about medication use are required to augment their awareness about their use.
Leveraging Tweets for Artificial Intelligence Driven Sentiment Analysis on the COVID-19 Pandemic
Authors: Alkhaldi Nora A. , Asiri Yousef , Mashraqi Aisha M. , Halawani Hanan T. , Abdel-Khalek Sayed , Mansour Romany F.
Published: May 2022

The COVID-19 pandemic has been a disastrous event that has elevated several psychological issues such as depression given abrupt social changes and lack of employment. At the same time, social scientists and psychologists have gained significant interest in understanding the way people express emotions and sentiments at the time of pandemics. During the rise in COVID-19 cases with stricter lockdowns, people expressed their sentiments on social media. This offers a deep understanding of human psychology during catastrophic events. By exploiting user-generated content on social media such as Twitter, people’s thoughts and sentiments can be examined, which aids in introducing health intervention policies and awareness campaigns. The recent developments of natural language processing (NLP) and deep learning (DL) models have exposed noteworthy performance in sentiment analysis. With this in mind, this paper presents a new sunflower optimization with deep-learning-driven sentiment analysis and classification (SFODLD-SAC) on COVID-19 tweets. The presented SFODLD-SAC model focuses on the identification of people’s sentiments during the COVID-19 pandemic. To accomplish this, the SFODLD-SAC model initially preprocesses the tweets in distinct ways such as stemming, removal of stopwords, usernames, link punctuations, and numerals. In addition, the TF-IDF model is applied for the useful extraction of features from the preprocessed data. Moreover, the cascaded recurrent neural network (CRNN) model is employed to analyze and classify sentiments. Finally, the SFO algorithm is utilized to optimally adjust the hyperparameters involved in the CRNN model. The design of the SFODLD-SAC technique with the inclusion of an SFO algorithm-based hyperparameter optimizer for analyzing people’s sentiments on COVID-19 shows the novelty of this study. The simulation analysis of the SFODLD-SAC model is performed using a benchmark dataset from the Kaggle repository. Extensive, comparative results report the promising performance of the SFODLD-SAC model over recent state-of-the-art models with maximum accuracy of 99.65%.
Ankle–Brachial Index Predicts Long-Term Renal Outcomes in Acute Stroke Patients
Authors: Lee Tsung-Lin , Chang Yu-Ming , Liu Chi-Hung , Su Hui-Chen , Sung Pi-Shan , Lin Sheng-Hsiang , Chen Chih-Hung
Published: May 2022

Renal dysfunction is common after stroke. We aimed to investigate the clinical predictability of the ankle–brachial index (ABI) and brachial-ankle pulse wave velocity (baPWV) on poststroke renal deterioration. A total of 956 consecutive participants with acute ischemic stroke between 1 July 2016, and 31 December 2017 were enrolled and a final of 637 patients were recruited for final analysis. By using the group-based trajectory model (GBTM), the patients’ renal function trajectories were grouped into the low, intermediate, and high categories (LC, IC, and HC). Significant deterioration in the slope was noted in the IC (p < 0.001) and LC (p = 0.002) groups but was nonsignificant in the HC (p = 0.998) group. Abnormal ABI (ABI ≤ 0.9) was independently related to LC (adjusted odds ratio: 2.40; 95% CI, 1.16–4.95; p = 0.019) and was also independently associated with increased risks of a ≥30% decline in eGFR (adjusted hazard ratio [aHR], 2.28; 95% CI, 1.29–4.05; p = 0.005), a doubling of serum creatinine (aHR, 3.60; 95% CI, 1.93–8.34; p < 0.001) and ESRD (HR, 3.28; 95% CI, 1.23–8.74; p = 0.018). However, baPWV did not have a significant relationship with any of the renal outcomes. Patients with a lower ABI during acute stroke should receive regular renal function tests and should be closely monitored to improve poststroke renal care.
Aspects of In Vitro Plant Tissue Culture and Breeding of Asparagus: A Review
Authors: Encina Carlos Lopez , Regalado José Javier
Published: May 2022

In vitro plant tissue culture and biotechnology used to assist and support the development of plant breeding when classical methods of propagation must be accelerated or it was necessary to overcome barriers inaccessible by classical approaches. In asparagus, to improve multiple breeding tasks, a high number of in vitro methods have been used, such as plant regeneration methods through organogenesis, embryogenesis, manipulation of ploidy, protoplast isolation, genetic manipulation (protoplast fusion, genetic transformation), embryo rescue and germplasm preservation (in vitro, in vitro slow growth, cryopreservation). Plant tissue culture methods can overcome multiple problems in asparagus breeding such as, barriers of self and cross-incompatibility between asparagus species through embryo rescue of interspecific hybrids and protoplast fusion or genetic transformation, introgression of new genes, clonal propagation of elite genotypes of asparagus, mass screening, and the generation of haploid and polyploid genotypes, among others, becoming the tool of choice for asparagus breeding programs. Some of these in vitro methods are still under development.
Nature and Mindfulness to Cope with Work-Related Stress: A Narrative Review
Authors: Menardo Elisa , Di Marco Donatella , Ramos Sara , Brondino Margherita , Arenas Alicia , Costa Patricia , Vaz de Carvalho Carlos , Pasini Margherita
Published: May 2022

In recent years, work-related stress has grown exponentially and the negative impact that this condition has on people’s health is considerable. The effects of work-related stress can be distinguished in those that affect workers (e.g., depression and anxiety) and those that affect the company (e.g., absenteeism and productivity). It is possible to distinguish two types of prevention interventions. Individual interventions aim at promoting coping and individual resilience strategies with the aim of modifying cognitive assessments of the potential stressor, thus reducing its negative impact on health. Mindfulness techniques have been found to be effective stress management tools that are also useful in dealing with stressful events in the workplace. Organizational interventions modify the risk factors connected to the context and content of the work. It was found that a restorative workplace (i.e., with natural elements) reduces stress and fatigue, improving work performance. Furthermore, practicing mindfulness in nature helps to improve the feeling of wellbeing and to relieve stress. In this paper, we review the role of mindfulness-based practices and of contact with nature in coping with stressful situations at work, and we propose a model of coping with work-related stress by using mindfulness in nature-based practices.
Emergency Medical Services Calls Analysis for Trend Prediction during Epidemic Outbreaks: Interrupted Time Series Analysis on 2020–2021 COVID-19 Epidemic in Lazio, Italy
Authors: Vinci Antonio , Pasquarella Amina , Corradi Maria Paola , Chatzichristou Pelagia , D’Agostino Gianluca , Iannazzo Stefania , Trani Nicoletta , Parafati Maria Annunziata , Palombi Leonardo , Ientile Domenico Antonio
Published: May 2022

(1) Background: During the COVID-19 outbreak in the Lazio region, a surge in emergency medical service (EMS) calls has been observed. The objective of present study is to investigate if there is any correlation between the variation in numbers of daily EMS calls, and the short-term evolution of the epidemic wave. (2) Methods: Data from the COVID-19 outbreak has been retrieved in order to draw the epidemic curve in the Lazio region. Data from EMS calls has been used in order to determine Excess of Calls (ExCa) in the 2020–2021 years, compared to the year 2019 (baseline). Multiple linear regression models have been run between ExCa and the first-order derivative (D’) of the epidemic wave in time, each regression model anticipating the epidemic progression (up to 14 days), in order to probe a correlation between the variables. (3) Results: EMS calls variation from baseline is correlated with the slope of the curve of ICU admissions, with the most fitting value found at 7 days (R2 0.33, p < 0.001). (4) Conclusions: EMS calls deviation from baseline allows public health services to predict short-term epidemic trends in COVID-19 outbreaks, and can be used as validation of current data, or as an independent estimator of future trends.
Effects of Health-Related Behaviors and Changes on Successful Aging among Indonesian Older People
Authors: Oktaviani Lisa Wahidatul , Hsu Hui-Chuan , Chen Yi-Chun
Published: May 2022

Whether changes in health behaviors can improve successful aging has not been well explored. The purpose of this study was to assess the effects of health-related behaviors and changes on successful aging in Indonesian older adults. Data were from the fourth and fifth waves of the Indonesia Family Life Survey (IFLS), the participants were aged 60 years and older and who completed both waves (n = 1289). Successful aging indicators were defined as no chronic diseases, no physical function difficulties, no depressive symptoms, intact cognitive function, with social support, and with social participation. Health-related behaviors focused on smoking, physical activities, and protein intake. A logistic regression analysis was conducted. The overall successful aging rate in 2007 was 23.6%, and it had decreased to 5.6% by 2014. There were gender differences in smoking, physical activities, and behavioral changes, including promoting increased physical activity, no smoking/smoking cessation, and adequate protein intake by older adults. Quitting smoking, performing medium physical activity, and increasing protein intake were protective factors for successful aging, but the effects of behavioral changes differed by gender. Health-related behaviors and changes may impact successful aging among older adults. A healthy lifestyle is suggested to be adopted as early as possible in one’s life course.
EEG Global Coherence in Scholar ADHD Children during Visual Object Processing
Authors: Hernández-Andrade Loyda , Hermosillo-Abundis Ana Cristina , Betancourt-Navarrete Brenda Lesly , Ruge Diane , Trenado Carlos , Lemuz-López Rafael , Pelayo-González Héctor Juan , López-Cortés Vicente Arturo , Bonilla-Sánchez María del Rosario , García-Flores Marco Antonio , Méndez-Balbuena Ignacio
Published: May 2022

Among neurodevelopmental disorders, attention deficit hyperactivity disorder (ADHD) is the main cause of school failure in children. Notably, visuospatial dysfunction has also been emphasized as a leading cause of low cognitive performance in children with ADHD. Consequently, the present study aimed to identify ADHD-related changes in electroencephalography (EEG) characteristics, associated with visual object processing in school-aged children. We performed Multichannel EEG recordings in 16-year-old children undergoing Navon’s visual object processing paradigm. We mapped global coherence during the processing of local and global visual stimuli that were consistent, inconsistent, or neutral. We found that Children with ADHD showed significant differences in global weighted coherence during the processing of local and global inconsistent visual stimuli and longer response times in comparison to the control group. Delta and theta EEG bands highlighted important features for classification in both groups. Thus, we advocate EEG coherence and low-frequency EEG spectral power as prospective markers of visual processing deficit in ADHD. Our results have implications for the development of diagnostic interventions in ADHD and provide a deeper understanding of the factors leading to low performance in school-aged children.
Evaluation of the Effect of Supervised Group Exercise on Self-Reported Sleep Quality in Pregnant Women with or at High Risk of Depression: A Secondary Analysis of a Randomized Controlled Trial
Authors: Broberg Lotte , Damm Peter , Frokjaer Vibe G. , Rosthøj Susanne , de Wolff Mie Gaarskjaer , Høgh Stinne , Tabor Ann , Hegaard Hanne Kristine
Published: May 2022

Poor sleep quality is common during pregnancy. Our objective was to evaluate the effect of supervised group physical exercise on self-reported sleep quality in pregnant women with or at high risk of depression, and secondly, to describe the association between sleep quality and psychological well-being during pregnancy and postpartum. This was a secondary analysis of a randomized controlled trial (n = 282) (NCT02833519) at Rigshospitalet, Denmark. Sleep quality was evaluated using the Pittsburgh Sleep Quality Index (PSQI), psychological well-being by the five-item WHO Well-Being Index (WHO-5). The intention-to-treat analysis showed no difference in mean global PSQI score neither at 29–34 weeks, 6.56 (95% CI: 6.05–7.07) in the intervention group and 7.00 (95% CI: 6.47–7.53) in the control group, p = 0.2, nor at eight weeks postpartum. Women with WHO-5 ≤ 50 reported higher mean global PSQI scores at baseline, 7.82 (95% CI: 7.26–8.38), than women with WHO-5 score > 50, mean 5.42 (95% CI: 5.02–5.82), p < 0.0001. A significant difference was also present post-intervention and eight weeks postpartum. No significant effect of group exercise regarding self-reported sleep quality was seen at 29–34 weeks of gestation or postpartum. Low psychological well-being was associated with poor sleep quality during pregnancy and postpartum.
Redevelopment and Examination of the Psychometric Properties of the Chinese Version of the Last 7-Day Sedentary Behaviour Questionnaire (SIT-Q-7d-Chi) in Hong Kong Older Adults
Authors: Leung Ka Man , Wong Ming Yu Claudia
Published: May 2022

(1) Background: This study examined the psychometric properties of the Chinese version of the Last 7-Day Sedentary Behaviour Questionnaire (SIT-Q-7d-Chi) in Hong Kong older adults; (2) Methods: Study 1 assessed the questionnaire’s test–retest reliability, and Study 2 examined its validity. Place the question addressed in a broad context and highlight the purpose of the study; (3) Results: In Study 1, 84 older adults (aged 60–90) completed the SIT-Q-7d-Chi twice over a 2-week interval, and in Study 2, 38 older adults (i) completed the SIT-Q-7d-Chi and the Sedentary Behaviour Questionnaire for Older Adults (SBQOA) and (ii) wore a waist-mounted accelerometer for 7 consecutive days. In Study 1, the SIT-Q-7d-Chi’s test–retest reliability (ICC = 0.91–0.99) was satisfactory, and adequate internal consistency was found for most domains of the SIT-Q-7d-Chi (Cronbach’s alpha value being 0.7 or above). Study 2′s results showed that the SIT-Q-7d-Chi results were significantly correlated with the SBQOA results, but not with the accelerometer results; (4) Conclusions: This study revealed the prevalence of sedentary behavior among Hong Kong’s senior citizens, which can be used as a reference to plan or evaluate a future sedentary behavior intervention for older persons, including identifying the content and intensity of activities.
A Deep Learning Approach to Estimate the Incidence of Infectious Disease Cases for Routinely Collected Ambulatory Records: The Example of Varicella-Zoster
Authors: Lanera Corrado , Baldi Ileana , Francavilla Andrea , Barbieri Elisa , Tramontan Lara , Scamarcia Antonio , Cantarutti Luigi , Giaquinto Carlo , Gregori Dario
Published: May 2022

The burden of infectious diseases is crucial for both epidemiological surveillance and prompt public health response. A variety of data, including textual sources, can be fruitfully exploited. Dealing with unstructured data necessitates the use of methods for automatic data-driven variable construction and machine learning techniques (MLT) show promising results. In this framework, varicella-zoster virus (VZV) infection was chosen to perform an automatic case identification with MLT. Pedianet, an Italian pediatric primary care database, was used to train a series of models to identify whether a child was diagnosed with VZV infection between 2004 and 2014 in the Veneto region, starting from free text fields. Given the nature of the task, a recurrent neural network (RNN) with bidirectional gated recurrent units (GRUs) was chosen; the same models were then used to predict the children’s status for the following years. A gold standard produced by manual extraction for the same interval was available for comparison. RNN-GRU improved its performance over time, reaching the maximum value of area under the ROC curve (AUC-ROC) of 95.30% at the end of the period. The absolute bias in estimates of VZV infection was below 1.5% in the last five years analyzed. The findings in this study could assist the large-scale use of EHRs for clinical outcome predictive modeling and help establish high-performance systems in other medical domains.
Evaluation of Active Renin Concentration in A Cohort of Adolescents with Primary Hypertension
Authors: Deja Anna , Skrzypczyk Piotr , Nowak Magdalena , Wrońska Małgorzata , Szyszka Michał , Ofiara Anna , Lesiak-Kosmatka Justyna , Stelmaszczyk-Emmel Anna , Pańczyk-Tomaszewska Małgorzata
Published: May 2022

Our study aimed to assess active renin concentration in children with primary hypertension. Thus, we evaluated active renin concentration, clinical parameters, office and ambulatory blood pressure, and biochemical parameters in 51 untreated adolescents with primary hypertension (median: 14.4 [interquartile range—IQR: 13.8–16.8] years) and 45 healthy adolescents. Active renin concentration did not differ between patients with hypertension and healthy children (median: 28.5 [IQR: 21.9–45.2] vs. 24.9 [IQR: 16.8–34.3] [pg/mL], p = 0.055). In the whole group of 96 children, active renin concentration correlated positively with serum potassium and office and ambulatory systolic and diastolic blood pressures. Among children with hypertension, patients with isolated systolic hypertension had lower renin concentration than patients with systolic-diastolic hypertension (26.2 [IQR: 18.6–34.2] vs. 37.8 [IQR: 27.0–49.6] [pg/mL], p = 0.014). The active renin concentration did not differ between patients with isolated systolic hypertension and healthy children. In multivariate analysis, diastolic blood pressure Z-score (beta = 0.238, 95 confidence interval [0.018–0.458], p = 0.035) was the only predictor of active renin concentration in the studied children. We concluded that active renin concentration is positively associated with blood pressure and potassium in children, and diastolic blood pressure was the strongest predictor of renin level. Patients with isolated systolic hypertension may differ from patients with systolic-diastolic hypertension in less severe activation of the renin-angiotensin-aldosterone system.