bluuebunny/crossref_metadata_2025 huggingface dataset:
Exact Solutions of Nonlinear Partial Differential Equations via the New Double Integral Transform Combined with Iterative Method
Authors: Ahmed Shams A. , Qazza Ahmad , Saadeh Rania
Published: May 2022

This article demonstrates how the new Double Laplace–Sumudu transform (DLST) is successfully implemented in combination with the iterative method to obtain the exact solutions of nonlinear partial differential equations (NLPDEs) by considering specified conditions. The solutions of nonlinear terms of these equations were determined by using the successive iterative procedure. The proposed technique has the advantage of generating exact solutions, and it is easy to apply analytically on the given problems. In addition, the theorems handling the mode properties of the DLST have been proved. To prove the usability and effectiveness of this method, examples have been given. The results show that the presented method holds promise for solving other types of NLPDEs.
Seven Sycoryctine Fig Wasp Species (Chalcidoidea: Pteromalidae) Associated with Dioecious Ficus hirta Inhabiting South China and Southeast Asia
Authors: Wong Da-Mien , Fan Songle , Yu Hui
Published: May 2022

Even though non-pollinating fig wasps are essential components in tropical and subtropical habitats, yet they are poorly described in the Oriental communities. This study presented seven new sycoryctine fig wasp species associated with Ficus hirta fig trees inhabiting South China and Southeast Asia. These new sycoryctine species belong to the genera Philotrypesis, Sycoryctes, and Sycoscapter. They can be easily distinguished by their adaptive morphologies such as face sculpture, body-color, and ovipositors. An identification key is provided to differentiate between them, and the relationships with their host fig trees are also discussed. The holotypes and paratypes are both deposited in the South China Botanical Garden, Chinese Academy of Sciences, China.
Overview and Update on Extracellular Vesicles: Considerations on Exosomes and Their Application in Modern Medicine
Authors: Di Bella Maria Antonietta
Published: May 2022

In recent years, there has been a rapid growth in the knowledge of cell-secreted extracellular vesicle functions. They are membrane enclosed and loaded with proteins, nucleic acids, lipids, and other biomolecules. After being released into the extracellular environment, some of these vesicles are delivered to recipient cells; consequently, the target cell may undergo physiological or pathological changes. Thus, extracellular vesicles as biological nano-carriers, have a pivotal role in facilitating long-distance intercellular communication. Understanding the mechanisms that mediate this communication process is important not only for basic science but also in medicine. Indeed, extracellular vesicles are currently seen with immense interest in nanomedicine and precision medicine for their potential use in diagnostic, prognostic, and therapeutic applications. This paper aims to summarize the latest advances in the study of the smallest subtype among extracellular vesicles, the exosomes. The article is divided into several sections, focusing on exosomes’ nature, characteristics, and commonly used strategies and methodologies for their separation, characterization, and visualization. By searching an extended portion of the relevant literature, this work aims to give a quick outline of advances in exosomes’ extensive nanomedical applications. Moreover, considerations that require further investigations before translating them to clinical applications are summarized.
Enhanced 3T3-L1 Differentiation into Adipocytes by Pioglitazone Pharmacological Activation of Peroxisome Proliferator Activated Receptor-Gamma (PPAR-γ)
Authors: Teixeira Catarina , Sousa André P. , Santos Inês , Rocha Ana Catarina , Alencastre Inês , Pereira Ana Cláudia , Martins-Mendes Daniela , Barata Pedro , Baylina Pilar , Fernandes Rúben
Published: May 2022

Despite the primary function of pioglitazone in antidiabetic treatment, this drug is a potent inducer of PPAR-γ, a crucial receptor that is involved in adipocyte differentiation. In this work, we propose an optimized methodology to enhance the differentiation of 3T3-L1 fibroblasts into adipocytes. This process is crucial for adipocyte secretome release, which is fundamental for understanding the molecular mechanisms that are involved in obesity for in vitro studies. To achieve this, a pioglitazone dose-response assay was determined over a range varying from 0 to 10 µM. Lipid accumulation was evaluated using Oil-Red-O. The results showed that 10 µM pioglitazone enhanced differentiation and increased secretome production. This secretome was then added into two cell lines: PC3 and RAW264.7. In the PC3 cells, an increase of aggressiveness was observed in terms of viability and proliferation, with the increase of anti-inflammatory cytokines. Conversely, in RAW264.7 cells, a reduction of viability and proliferation was observed, with a decrease in the overexpression of pro-inflammatory cytokines. Overall, the present work constitutes an improved method for adipocyte secretome production that is suitable for experimental biology studies and that could help with our understanding of the molecular mechanisms underlying adiposity influence in other cells.
Cell Biology of Galectins: Novel Aspects and Emerging Challenges
Authors: Timoshenko Alexander V.
Published: May 2022

Galectins are a family of soluble β-galactoside-binding proteins with diverse glycan-dependent and glycan-independent functions outside and inside the cell [...]
Gut Microbiota and Associated Mucosal Immune Response in Eosinophilic Granulomatosis with Polyangiitis (EGPA)
Authors: Niccolai Elena , Bettiol Alessandra , Baldi Simone , Silvestri Elena , Di Gloria Leandro , Bello Federica , Nannini Giulia , Ricci Federica , Nicastro Maria , Ramazzotti Matteo , Vaglio Augusto , Bartolucci Gianluca , Emmi Giacomo , Amedei Amedeo , Prisco Domenico
Published: May 2022

Eosinophilic granulomatosis with polyangiitis (EGPA) is an anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. A genome-wide association study showed a correlation between ANCA-negative EGPA and variants of genes encoding proteins with intestinal barrier functions, suggesting that modifications of the mucosal layer and consequent gut dysbiosis might be involved in EGPA pathogenesis. Here, we characterized the gut microbiota (GM) composition and the intestinal immune response in a cohort of EGPA patients. Faeces from 29 patients and 9 unrelated healthy cohabitants were collected, and GM and derived metabolites’ composition were compared. Seven intestinal biopsies from EGPA patients with gastrointestinal manifestations were analysed to assess the T-cell distribution and its correlation with GM and EGPA clinical and laboratory features. No significant differences in GM composition, nor in the total amount of faecal metabolites, emerged between patients and controls. Nevertheless, differences in bacterial taxa abundances and compositional GM-derived metabolites profile were observed. Notably, an enrichment of potential pathobionts (Enterobacteriacee and Streptococcaceae) was found in EGPA, particularly in patients with active disease, while lower levels were found in patients on immunosuppression, compared with non-immunosuppressed ones. Significantly lower amounts of hexanoic acid were found in patients, compared to controls. The analysis of the immune response in the gut mucosa revealed a high frequency of IFN-γ/IL-17-producing T lymphocytes, and a positive correlation between EGPA disease activity and intestinal T-cell levels. Our data suggest that an enrichment in potential intestinal pathobionts might drive an imbalanced inflammatory response in EGPA.
Effects of Thyroid Hormones on Lipid Metabolism Pathologies in Non-Alcoholic Fatty Liver Disease
Authors: Liao Chia-Jung , Huang Po-Shuan , Chien Hui-Tzu , Lin Tzu-Kang , Yeh Chau-Ting , Lin Kwang-Huei
Published: May 2022

The typical modern lifestyle contributes to the development of many metabolic-related disorders, as exemplified by metabolic syndrome. How to prevent, resolve, or avoid subsequent deterioration of metabolic disturbances and the development of more serious diseases has become an important and much-discussed health issue. Thus, the question of the physiological and pathological roles of thyroid hormones (THs) in metabolism has never gone out of fashion. Although THs influence almost all organs, the liver is one of the most important targets as well as the hub of metabolic homeostasis. When this homeostasis is out of balance, diseases may result. In the current review, we summarize the common features and actions of THs, first focusing on their effects on lipid metabolism in the liver. In the second half of the review, we turn to a consideration of non-alcoholic fatty liver disease (NAFLD), a disease characterized by excessive accumulation of fat in the liver that is independent of heavy alcohol consumption. NAFLD is a growing health problem that currently affects ~25% of the world’s population. Unfortunately, there are currently no approved therapies specific for NAFLD, which, if left uncontrolled, may progress to more serious diseases, such as cirrhosis or liver cancer. This absence of effective treatment can also result in the development of non-alcoholic steatohepatitis (NASH), an aggressive form of NAFLD that is the leading cause of liver transplantation in the United States. Because THs play a clear role in hepatic fat metabolism, their potential application in the prevention and treatment of NAFLD has attracted considerable research attention. Studies that have investigated the use of TH-related compounds in the management of NAFLD are also summarized in the latter part of this review. An important take-home point of this review is that a comprehensive understanding of the physiological and pathological roles of THs in liver fat metabolism is possible, despite the complexities of this regulatory axis—an understanding that has clinical value for the specific management of NAFLD.
Bovine Milk Fat Intervention in Early Life and Its Impact on Microbiota, Metabolites and Clinical Phenotype: A Multi-Omics Stacked Regularization Approach
Authors: Pereira João , Bresser Lucas R. F. , Riel Natal van , Looijesteijn Ellen , Schoemaker Ruud , Ulfman Laurien H. , Jeurink Prescilla , Karaglani Eva , Manios Yannis , Brouwer Rutger W. W. , Ijcken Wilfred F. J. van , Levin Evgeni
Published: May 2022

The integration and analysis of multi-omics modalities is an important challenge in bioinformatics and data science in general. A standard approach is to conduct a series of univariate tests to determine the significance for each parameter, but this underestimates the connected nature of biological data and thus increases the number of false-negative errors. To mitigate this issue and to understand how different omics’ data domains are jointly affected, we used the Stacked Regularization model with Bayesian optimization over its full parameter space. We applied this approach to a multi-omics data set consisting of microbiota, metabolites and clinical data from two recent clinical studies aimed at detecting the impact of replacing part of the vegetable fat in infant formula with bovine milk fat on healthy term infants. We demonstrate how our model achieves a high discriminative performance, show the advantages of univariate testing and discuss the detected outcome in its biological context.
Developing a Method to Connect Thermal Physiology in Animals and Plants to the Design of Energy Efficient Buildings
Authors: Imani Negin , Vale Brenda
Published: May 2022

The literature shows that translating the thermal adaptation mechanisms of biological organisms to building design solutions can improve energy performance. In the context of bio-inspired thermoregulation several worthwhile attempts have been made to develop a framework for finding relevant thermal adaptation mechanisms in nature as inspiration for architectural design. However, almost all of these have followed a solution-based approach despite the problem-solving nature of architectural design. Given this, this research set out to take a problem-based approach to biomimetic design. The aim was to investigate the most effective way of accessing biological thermoregulatory solutions to assist architects in finding relevant biological inspirations for the thermal design of buildings. This required the development of an optimal structure for categorizing thermoregulatory mechanisms that could then be used as part of a framework for finding appropriate mechanisms for a particular architectural design problem. This development began with a three-step literature review to find, study, generalize and categorize a comprehensive list of thermal adaptation mechanisms used by animals and plants. This article describes how this literature review was carried out leading to the identification of nine main themes which were analysed for their practicality in informing the structure of the proposed framework. The selected themes were built around the common aspects of biology and architecture, and hence facilitated the categorization of biological thermoregulation mechanisms. This article thus explains the steps taken to develop a structure for generalizing and categorizing thermal adaptation strategies in nature. This article does not report on the list of thermal adaptation mechanisms identified in step 2 of the literature review. Instead, it presents the literature review workflow with a focus on step 3. Given that, discussion of the thermal adaptation mechanisms falls outside the scope of this article.
Phosphoprotein Detection in Sweat Realized by Intercalation Structure 2D@3D g-C3N4@Fe3O4 Wearable Sensitive Motif
Authors: Qiao Yuting , Qiao Lijuan , Zhao Peize , Zhang Peng , Wu Fanbin , Zhang Jiahui , Gao Li , Liu Bingxin , Zhang Lei
Published: May 2022

Abnormal protein phosphorylation in sweat metabolites is closely related to cancer, cardiovascular disease, and other diseases. The real-time monitoring of phosphoproteins in sweat is significant for early monitoring of disease biomarkers. Here, a high-efficiency electrochemical sensor for phosphoprotein in sweat was realized by 2D@3D g-C3N4@Fe3O4 with intercalation structure. Common phosphoprotein β-Casein was selected to demonstrate the platform’s functionalities. The detection limit of g-C3N4@Fe3O4 could be as low as 9.7 μM, and the detection range was from 0.01 mg/mL to 1 mg/mL. In addition, the sensing platform showed good selectivity, reproducibility, and stability. We also investigated the effects of interface structure on adsorption properties and electronic properties of the g-C3N4 and Fe3O4 heterostructure using DFT. More electrons from Fe3O4 were transferred to g-C3N4, which increased the electrons in the energy band of N atoms and promoted the formation of stable N-H bonds with H atoms in phosphoproteins. We demonstrated phosphoprotein sensor functionality by measuring the phosphoprotein in human sweat during exercising. This work realizes a sensing platform for noninvasive and continuous detection of sweat phosphoproteins in wearable devices.
Mental Health of Mothers of Children with Neurodevelopmental and Genetic Disorders in Pakistan
Authors: Neoh Michelle Jin Yee , Airoldi Livia , Arshad Zarah , Bin Eid Wasmiah , Esposito Gianluca , Dimitriou Dagmara
Published: May 2022

This study examined maternal mental health in mothers of children with neurodevelopmental and genetic disorders in Pakistan; maternal education and affiliate stigma were also tested. It was hypothesised that mothers of children with neurodevelopmental and genetic disorders would experience high levels of depression, anxiety and affiliate stigma, and that these variables would be mediated by the level of maternal education. Seventy-five mothers of children with neurodevelopmental and genetic disorders were recruited from “Special Needs” schools in Lahore and Islamabad. The results showed that the majority of mothers were clinically depressed and a large majority of mothers were clinically anxious. The distribution of mothers with clinical anxiety was significantly different among mothers with different levels of education (p < 0.05). Furthermore, mothers with higher levels of education had less affiliate stigma. This result suggests that maternal education may be a protective factor of mental health in mothers of children with neurodevelopmental and genetic disorders. Limitations, implications and future research are discussed.
Burn Them Right! Determining the Optimal Temperature for the Purification of Carbon Materials by Combustion
Authors: Picheau Emmanuel , Hof Ferdinand , Derré Alain , Amar Sara , Noé Laure , Monthioux Marc , Pénicaud Alain
Published: May 2022

A new purification procedure for carbon nanoforms is proposed. It was tested on multiwall carbon nanotubes (MWCNTs) prepared by arc discharge, which is among the most challenging of cases due to the chemical and structural similarity between the MWCNTs and most of the impurities to be removed. Indeed, the various methods for synthesizing carbon nanoforms lead to a distribution of carbonaceous products, such as carbon shells, carbon spheres, fullerenes, and a variety of other species. Thus, many strategies to purify the desired products have been developed. Among the most successful ones, thermal oxidation (combustion) seems particularly efficient. To be successful while preserving a reasonable amount of MWCNTs, the combustion temperature has to be carefully selected. Moreover, the ideal combustion temperature does not only depend on the material to be treated but also on the overall system used to perform the reaction, including the reactor type and the parameters of the gaseous reactant. Typically, the optimization of the purification relies on multiple experiments and analysis of the products. However, to the best of our knowledge, a strategy to determine a priori the most suitable temperature has not been reported yet. We demonstrate here that a thermogravimetric method, namely the constant decomposition rate thermal analysis (CRTA), is particularly well adapted to answer this question. An isothermal treatment based on the results obtained from a CRTA program allowed arc-MWCNTs to be successfully purified from graphenic shells while optimizing the yield of the MWCNTs. This strategy is believed to be valuable not only for purifying MWCNTs but also for the purification of other carbonaceous forms, including new carbon nanoforms.
Photodegradation of Pharmaceutical Pollutants: New Photocatalytic Systems Based on 3D Printed Scaffold-Supported Ag/TiO2 Nanocomposite
Authors: Bergamonti Laura , Graiff Claudia , Bergonzi Carlo , Potenza Marianna , Reverberi Cinzia , Ossiprandi Maria Cristina , Lottici Pier Paolo , Bettini Ruggero , Elviri Lisa
Published: May 2022

Due to the release of active pharmaceutical compounds in wastewater and their persistence in the environment, dangerous consequences can develop in the aquatic and terrestrial organisms. Chitosan/Ag/TiO2 3D printed scaffolds, at different Ag nanoparticle concentrations (10, 100, 1000 ppm) are investigated here as promising materials for photocatalytic degradation under the UV–Vis irradiation of pharmaceutical compounds in wastewater. As target drugs, amoxicillin, paracetamol and their 1:1 mix were selected. Ag nanoparticles increase the photocatalytic efficiency of the system based on titanium dioxide embedded in the chitosan scaffold: in the presence of Chitosan/Ag100/TiO2, the selected pharmaceuticals (PhCs), monitored by UV–Vis spectroscopy, are completely removed in about 2 h. The photodegradation products of the PhCs were identified by Liquid Chromatography–Mass Spectroscopy and assessed for their toxicological impact on six different bacterial strains: no antibacterial activity was found towards the tested strains. This new system based on Ag/TiO2 supported on 3D chitosan scaffolds may represent an effective strategy to reduce wastewater pollution by emerging contaminants.
Unraveling the Pathogenesis of Asthma and Chronic Obstructive Pulmonary Disease Overlap: Focusing on Epigenetic Mechanisms
Authors: Chen Yung-Che , Chang Yu-Ping , Huang Kuo-Tung , Hsu Po-Yuan , Hsiao Chang-Chun , Lin Meng-Chih
Published: May 2022

Asthma and COPD overlap (ACO) is characterized by patients presenting with persistent airflow limitation and features of both asthma and COPD. It is associated with a higher frequency and severity of exacerbations, a faster lung function decline, and a higher healthcare cost. Systemic inflammation in COPD and asthma is driven by type 1 T helper (Th1) and Th2 immune responses, respectively, both of which may contribute to airway remodeling in ACO. ACO-related biomarkers can be classified into four categories: neutrophil-mediated inflammation, Th2 cell responses, arachidonic acid-eicosanoids pathway, and metabolites. Gene–environment interactions are key contributors to the complexity of ACO and are regulated by epigenetic mechanisms, including DNA methylation, histone modifications, and non-coding RNAs. Thus, this review focuses on the link between epigenetics and ACO, and outlines the following: (I) inheriting epigenotypes without change with environmental stimuli, or epigenetic changes in response to long-term exposure to inhaled particles plus intermittent exposure to specific allergens; (II) epigenetic markers distinguishing ACO from COPD and asthma; (III) potential epigenetic drugs that can reverse oxidative stress, glucocorticoid insensitivity, and cell injury. Improved understanding of the epigenetic regulations holds great value to give deeper insight into the mechanisms, and clarify their implications for biomedical research in ACO.
Therapeutic Validation of GEF-H1 Using a De Novo Designed Inhibitor in Models of Retinal Disease
Authors: Mills Clare , Hemkemeyer Sandra A. , Alimajstorovic Zerin , Bowers Chantelle , Eskandarpour Malihe , Greenwood John , Calder Virginia , Chan A. W. Edith , Gane Paul J. , Selwood David L. , Matter Karl , Balda Maria S.
Published: May 2022

Inflammation and fibrosis are important components of diseases that contribute to the malfunction of epithelia and endothelia. The Rho guanine nucleotide exchange factor (GEF) GEF-H1/ARHGEF-2 is induced in disease and stimulates inflammatory and fibrotic processes, cell migration, and metastasis. Here, we have generated peptide inhibitors to block the function of GEF-H1. Inhibitors were designed using a structural in silico approach or by isolating an inhibitory sequence from the autoregulatory C-terminal domain. Candidate inhibitors were tested for their ability to block RhoA/GEF-H1 binding in vitro, and their potency and specificity in cell-based assays. Successful inhibitors were then evaluated in models of TGFβ-induced fibrosis, LPS-stimulated endothelial cell-cell junction disruption, and cell migration. Finally, the most potent inhibitor was successfully tested in an experimental retinal disease mouse model, in which it inhibited blood vessel leakage and ameliorated retinal inflammation when treatment was initiated after disease diagnosis. Thus, an antagonist that blocks GEF-H1 signaling effectively inhibits disease features in in vitro and in vivo disease models, demonstrating that GEF-H1 is an effective therapeutic target and establishing a new therapeutic approach.
Redistribution of TNF Receptor 1 and 2 Expression on Immune Cells in Patients with Bronchial Asthma
Authors: Alshevskaya Alina , Zhukova Julia , Kireev Fedor , Lopatnikova Julia , Evsegneeva Irina , Demina Daria , Nepomniashchikch Vera , Gladkikh Victor , Karaulov Alexander , Sennikov Sergey
Published: May 2022

Background: The co-expression patterns of type 1 and 2 tumor necrosis factor (TNF)-α membrane receptors (TNFR1/TNFR2) are associated with the presence, stage, and activity of allergic diseases. The aim of this study was to assess the expression levels and dynamics of TNFRs on immune cells and to assess associations between their expression and severity of bronchial asthma (BA). Methods: Patients with severe (n = 8), moderate (n = 10), and mild (n = 4) BA were enrolled. As a comparison group, data from 46 healthy volunteers (HV) were accessed. Co-expression of TNFR1/2 was evaluated as a percentage of cells and the number of receptors of each type per cell. Multivariate logistic regression analysis was used to identify diagnostic biomarkers of BA. Results: More than 90% of the monocytes in patients with mild BA were TNFR1+TNFR2+ but had significantly lower TNFR1 expression density compared with HV (7.82- to 14.08-fold, depending on disease severity). Lower percentages of the TNFR+ B-lymphocytes were observed in combination with significantly lower receptors density in BA compared with HV (2.59- to 11.64-fold for TNFR1 and 1.72- to 3.4-fold for TNFR2, depending on disease severity). The final multivariate model for predicting the presence of BA included the percentage of double-positive CD5+ B-lymphocytes and average number of TNFR1 molecules expressed on cytotoxic naive T-lymphocytes and T-helper cells (R2 = 0.87). Conclusions: The co-expression patterns of TNFRs on immune cells in BA differed significantly compared with HV. The expression differences were associated with disease severity. TNFR1 expression changes were key parameters that discriminated patients with BA from those with HV.
Homocysteine Metabolism Pathway Is Involved in the Control of Glucose Homeostasis: A Cystathionine Beta Synthase Deficiency Study in Mouse
Authors: Cruciani-Guglielmacci Céline , Meneyrol Kelly , Denom Jessica , Kassis Nadim , Rachdi Latif , Makaci Fatna , Migrenne-Li Stéphanie , Daubigney Fabrice , Georgiadou Eleni , Denis Raphaël G. , Rodriguez Sanchez-Archidona Ana , Paul Jean-Louis , Thorens Bernard , Rutter Guy A. , Magnan Christophe , Le Stunff Hervé , Janel Nathalie
Published: May 2022

Cystathionine beta synthase (CBS) catalyzes the first step of the transsulfuration pathway from homocysteine to cystathionine, and its deficiency leads to hyperhomocysteinemia (HHcy) in humans and rodents. To date, scarce information is available about the HHcy effect on insulin secretion, and the link between CBS activity and the setting of type 2 diabetes is still unknown. We aimed to decipher the consequences of an inborn defect in CBS on glucose homeostasis in mice. We used a mouse model heterozygous for CBS (CBS+/−) that presented a mild HHcy. Other groups were supplemented with methionine in drinking water to increase the mild to intermediate HHcy, and were submitted to a high-fat diet (HFD). We measured the food intake, body weight gain, body composition, glucose homeostasis, plasma homocysteine level, and CBS activity. We evidenced a defect in the stimulated insulin secretion in CBS+/− mice with mild and intermediate HHcy, while mice with intermediate HHcy under HFD presented an improvement in insulin sensitivity that compensated for the decreased insulin secretion and permitted them to maintain a glucose tolerance similar to the CBS+/+ mice. Islets isolated from CBS+/− mice maintained their ability to respond to the elevated glucose levels, and we showed that a lower parasympathetic tone could, at least in part, be responsible for the insulin secretion defect. Our results emphasize the important role of Hcy metabolic enzymes in insulin secretion and overall glucose homeostasis.
The Impact of Air Pollution on Gut Microbiota and Children’s Health: An Expert Consensus
Authors: Fadlyana Eddy , Soemarko Dewi Sumaryani , Endaryanto Anang , Haryanto Budi , Darma Andy , Dewi Dian Kusuma , Chandra Dian Novita , Hartono Budi , Buftheim Sonia , Wasito Erika , Sundjaya Tonny , Basrowi Ray Wagiu
Published: May 2022

Air pollution is an unseen threat to children’s health because it may increase the risk of respiratory infection, atopy, and asthma, and also alter gut microbiota compositions. The impact of air pollution on children’s health has not been firmly established. A literature review followed by a series of discussions among experts were performed to develop a theoretical framework on how air pollution could affect various bodily organs and functions in children. We invited experts from different backgrounds, such as paediatricians, nutritionists, environmental health experts, and occupational health experts, to provide their views on this matter. This report summarizes the discussion of multidisciplinary experts on the impact of air pollution on children’s health. The report begins with a review of air pollution’s impact on allergy and immunology, neurodevelopment, and cardiometabolic risks, and ends with the conceptualization of a theoretical framework. While the allergic and immunological pathway is one of the most significant pathways for air pollution affecting children’s health in which microbiotas also play a role, several pathways have been proposed regarding the ability to affect neurodevelopment and cardiometabolic risk. Further research is required to confirm the link between air pollution and the gut microbiota pathway.
Bullying and Health Related Quality of Life among Adolescents—A Systematic Review
Authors: Dubey Viney Prakash , Kievišienė Justina , Rauckiene-Michealsson Alona , Norkiene Sigute , Razbadauskas Artūras , Agostinis-Sobrinho Cesar
Published: May 2022

Health-related quality of life is among global health goals not only in adulthood but also in childhood and adolescence. Being a multi-component construct, health-related quality of life covers various domains, such as physical and psychological wellbeing and social and environmental areas. Bullying might significantly influence those domains especially in adolescence, a period of life when numerous personal and interpersonal transformations are experienced. Therefore, the aim of the current systematic review was to provide a comprehensive overview of the relationship of bullying with the health-related quality of adolescents’ lives. An electronic literature search was performed using PubMed, Embase, and Cochrane Library, and 3621 full-text articles were identified. After a selection process, 12 studies covering diagnosis, prevention and treatment for each of the three sections “adolescents”, “health related quality of life” and “bullying” were reviewed. An overall reduction in health-related quality of life in regard to bullying appeared from the studies analyzed, as well as a decline in adolescent mental health. Different bullying types were identified as causing harm to various adolescents‘ health-related quality of life domains. These findings may contribute to effective bullying management in schools and/or societal settings, and inform intervention strategies for maintaining the quality of life of adolescents being bullied.
Conformity between Pacifier Design and Palate Shape in Preterm and Term Infants Considering Age-Specific Palate Size, Facial Profile and Lip Thickness
Authors: Sistenich Gwendolin , Middelberg Claudius , Stamm Thomas , Dirksen Dieter , Hohoff Ariane
Published: May 2022

This retrospective case-control study is the first to examine the spatial conformity between pacifiers and palates in 39 preterm infants (12 females, 27 males) and 34 term infants (19 females, 15 males), taking into account the facial-soft-tissue profile and thickness. The shape of 74 available pacifiers was spatially matched to the palate, and conformity was examined using width, height, and length measurements. In summary, the size concept of pacifiers is highly variable and does not follow a growth pattern, like infant palates do. Pacifiers are too undersized in width, length, and height to physiologically fit the palate structures from 0 to 14 months of age. There are two exceptions, but only for premature palates: the palatal depth index at 9–11 months of age, which has no clinical meaning, and the nipple length at <37 weeks of age, which bears a resemblance to the maternal nipple during non-nutritive sucking. It can be concluded that the age-size concept of the studied pacifiers does not correspond to any natural growth pattern. Physiologically aligned, pacifiers do not achieve the age-specific dimensions of the palate. The effects attributed to the products on oral health in term infants cannot be supposed.
Long-Term Health Associated with Small and Large for Gestational Age Births among Young Thai Adults
Authors: Suhag Alisha , Rerkasem Amaraporn , Kulprachakarn Kanokwan , Parklak Wason , Angkurawaranon Chaisiri , Rerkasem Kittipan , Derraik José G. B.
Published: May 2022

We examined the long-term health outcomes associated with being born small for gestational age (SGA) or large for gestational age (LGA). A total of 632 young adults aged ≈20.6 years were recruited from a longitudinal study (Chiang Mai, Thailand) in 2010: 473 born appropriate for gestational age (AGA), 142 SGA, and 17 LGA. The clinical assessments included anthropometry, blood pressure (BP), lipid profile, and an oral glucose tolerance test (OGTT). Young adults born SGA were 1.8 and 3.2 cm shorter than AGA (p = 0.0006) and LGA (p = 0.019) participants, respectively. The incidence of short stature was 8% among SGA compared with 3% in AGA and no cases among LGA participants, with the adjusted relative risk (aRR) of short stature among SGA 2.70 times higher than that of AGA counterparts (p = 0.013). SGA participants also had a 2 h glucose 7% higher than that of the AGA group (105 vs. 99 mg/dL; p = 0.006). Young adults born LGA had a BMI greater by 2.42 kg/m2 (p = 0.025) and 2.11 kg/m2 (p = 0.040) than those of SGA and AGA, respectively. Thus, the rate of overweight/obesity was 35% in the LGA group compared with 14.2% and 16.6% of SGA and AGA groups, respectively, with corresponding aRR of overweight/obesity of 2.95 (p = 0.011) and 2.50 (p = 0.017), respectively. LGA participants had markedly higher rates of BP abnormalities (prehypertension and/or hypertension) with an aRR of systolic BP abnormalities of 2.30 (p = 0.023) and 2.79 (p = 0.003) compared with SGA and AGA groups, respectively. Thai young adults born SGA had an increased risk of short stature and displayed some impairment in glucose metabolism. In contrast, those born LGA were at an increased risk of overweight/obesity and elevated blood pressure. The long-term follow-up of this cohort is important to ascertain whether these early abnormalities accentuate over time, leading to overt cardiometabolic conditions.
Early Detection of Corrosion-Induced Concrete Micro-cracking by Using Nonlinear Ultrasonic Techniques: Possible Influence of Mass Transport Processes
Authors: Climent Miguel-Ángel , Miró Marina , Eiras Jesús-Nuño , Poveda Pedro , de Vera Guillem , Segovia Enrique-Gonzalo , Ramis Jaime
Published: May 2022

This work presents results allowing an unequivocal correlation of the observations of strong nonlinear elastic features of ultrasonic waves (values of the nonlinearity parameters exceeding the thresholds corresponding to undamaged states), with the critical events of the corrosion-induced surface cracking of reinforced cement-mortar specimens. These observations point to the possibility of the early detection of cracking using nonlinear ultrasonic (NLU) techniques. Experimental evidence is presented on the existence of active net mass transport processes, due to wick action, in the course of the corrosion tests, in the experimental conditions of this work. These phenomena might explain the observed abrupt shifting of the nonlinear parameter values (typically increasing and then decreasing post-peak, even reaching values typical of the undamaged state), and, partially, the high variability obtained for the values of the nonlinear parameters in damaged (cracked) states. Finally, some consequences are derived from the point of view of use of the NLU techniques in engineering practice, i.e., in surveys aimed at evaluating reinforced concrete structures affected by corrosion.
Characterization of Some Physical and Photocatalytic Properties of CuO Nanofilms Synthesized by a Gentle Chemical Technique
Authors: Aroussi Soumia , Dahamni Mohamed Amine , Ghamnia Mostefa , Tonneau Didier , Fauquet Carole
Published: May 2022

Pure and Li-doped CuO nanofilms were synthesized on heated glass substrates using the spray-pyrolysis technique. The deposited pure CuO nanofilms were achieved at a precursor molarity of 0.2 M using a solution prepared from copper nitrate trihydrate (Cu(NO3)2·3H2O). Doped Li–CuO nanofilms were obtained using several doping concentrations (3, 6, 9, 12 and 15%) by adding a solution prepared from lithium nitrate (LiNO3). The pure and Li–CuO samples were investigated by different techniques. XRD revealed three dominant peaks (-111), (111) and (211), which are the properties of monoclinic CuO. The increase in Li-doping concentration showed the appearance of other peaks of low intensities detected at 2θ ranging from 49 to 68°. AFM images showed a textured and inhomogeneous surface composed of spherical grains whose size decreased with increasing Li doping. UV–visible spectroscopy showed that the CuO samples were of low transparency; the transmittance was less than 50%. The band-gap energy determined from Tauc’s equation plot increased from 2.157 to 3.728 eV with the increase in Li doping. These values correspond well to the band gap of semiconducting CuO. The photocatalytic properties were accelerated by Li doping, as revealed by the discoloration of aqueous methylene-blue (MB) solution under ultraviolet irradiation.
Variational Formulations and Isogeometric Analysis of Timoshenko–Ehrenfest Microbeam Using a Reformulated Strain Gradient Elasticity Theory
Authors: Yin Shuohui , Xiao Zhibing , Liu Jingang , Xia Zixu , Gu Shuitao
Published: May 2022

This paper presents a novel non-classical Timoshenko–Ehrenfest beam model based on a reformulated strain gradient elasticity theory. The strain gradient effect, couple stress effect, and velocity gradient effect for vibration are included in the new model by only one material length scale parameter for each. The variational formulation and Hamilton’s principle are applied to derive the governing equations and boundary conditions. Both an analytical solution and an isogeometric analysis approach are proposed for static bending and free vibration of the microbeam. A non-uniform rational B-splines (NURBS) isogeometric analysis with high-order continuity can effectively fulfill the higher derivatives of the displacement variables in the reformulated gradient beam model. Convergence studies and comparisons to the corresponding analytical solutions verify the model’s performance and accuracy. Finally, different boundary conditions, material length scale parameters, and beam thicknesses are investigated in order to certify the applicability of the proposed approach.
Crystallinity of Bitumen via WAXD and DSC and Its Effect on the Surface Microstructure
Authors: Pipintakos Georgios , Soenen Hilde , Goderis Bart , Blom Johan , Lu Xiaohu
Published: May 2022

It is well documented that most bituminous binders contain crystallisable material. This crystallisable fraction, often referred to as paraffinic or natural wax, is associated with the bitumen’s origin and has an influence on its rheological performance. In the literature, Differential Scanning Calorimetry (DSC) is the primary technique used to determine the melting and crystallisation behaviour of this waxy fraction. However, in bitumen, thermal transitions in DSC are typically very broad and can be combined with recrystallisation effects upon reheating. This work explores the potential crystallisation and melting process of a waxy and a wax-free bitumen via three different approaches: DSC, Wide-Angle X-ray Diffraction (WAXD) and Confocal Laser Scanning Microscopy (CLSM). The findings reveal that the DSC transitions of the waxy bitumen are in good agreement with the corresponding occurrence of WAXD signals and to some extent with the formation and disappearance of the surface microstructures which were followed at two cooling and heating rates. WAXD results additionally demonstrate that the crystalline material in bitumen is organised in an orthorhombic unit cell, typical for straight chain aliphatic structures. On the other hand, DSC and WAXD support the lack of crystallinity for the wax-free bitumen which could explain its featureless CLSM surface. Overall, the originality of this work resides in the disclosure of connections between crystallographic properties, thermal transitions and the surface micromorphology of bitumen.
Perturbed Angular Correlation Technique at ISOLDE/CERN Applied for Studies of Hydrogenated Titanium Dioxide (TiO2): Observation of Cd-H Pairs
Authors: Zyabkin Dmitry V. , Schell Juliana , Correia João G. M. , Vetter Ulrich , Schaaf Peter
Published: May 2022

Profound understanding of the local electronic and defect structure in semiconductors always plays a vital role in the further developing of applications of such materials. In the present work an investigation of the electronic structure in hydrogenated TiO2 (rutile) thin films is conducted by virtue of Time-Differential γ-γ Perturbed Angular Correlation spectroscopy (TDPAC or PAC) with 111mCd/Cd isotope, produced and implanted at ISOLDE/CERN. The measurements were performed at 581 K as a function of the temperature of the samples during hydrogenation. Despite the fact, that rutile single crystals usually show the presence of two local environments, when are studies with Cd/In isotopes, the current pristine thin films sample had a single electric field gradient. Upon various degrees of hydrogenation, Cd probe atoms showed underwent alterations, resulting in up to 3 different local surroundings, generally with high electric field gradients. Broad EFG distributions are likely due to randomly distributed point defects in the neighbourhood of Cd acceptors. Observed results suggest that hydrogenations performed at RT and 423 K are not able to promote unique defect configurations, while in the range of 473-573 K the formation of such configurations is observed. Therefore, one may assume that the formation of Cd-defect complexes (Cd-H pairs) is temperature enhanced. At higher levels of hydrogenation (663 K), the samples become partly amorphous that further hinders any atomistic studies with strong damped PAC spectra. Cd-H complexes seem to be stable up to annealing up to 581 K annealing. The obtained results give a deep insight into complex hydrogen defects, their interactions and bond formations with Cd acceptor.
Wrinkle-Shaped Nickel Sulfide Grown on Three-Dimensional Nickel Foam: A Binder-Free Electrode Designed for High-Performance Electrochemical Supercapacitor Applications
Authors: Ansari Sajid Ali , Kotb Hicham Mahfoz , Ahmad Mohamad M.
Published: May 2022

Recently, three-dimensional nickel foam (3D-Nf) has been increasingly studied; however, further modifications in nanoscale surface modification are necessary for particular applications. In this work, three-dimensional hierarchically porous nanogranular NiS (NiS-3D-Nf) and wrinkle-shaped NiS (w-NiS-3D-Nf) structures were fabricated directly on nickel foam by a simple one-step solvothermal process using two different solvents. Several characterization techniques, including X-ray diffraction pattern, X-ray photoelectron spectroscopy, and scanning electron microscopy, were used to characterize the samples’ properties. To prove their applicability, supercapacitor electrodes were tested directly in a three-electrode assembly cell. The resulting w-NiS-3D-Nf electrodes exhibited greater capacitive activity than the NiS-3D-Nf electrodes. The optimized w-NiS-3D-Nf electrodes delivered an excellent specific capacitance of 770 Fg−1, at a current density of 1 Ag−1, compared with the NiS-3D-Nf electrodes (162.0 Fg−1 @ 1 Ag−1), with a cyclic stability of over 92.67% capacitance retention after 2200 cycles. The resultant unique structure with integrated hierarchical three-dimensional configuration can not only enhance abundant accessible surface areas but also produce strong adhesion to the 3D-Nf, facilitating the fast transportation of ions and electrons for the electrochemical reaction via the conductive 3D-Nf. This set of results suggests that the modification of 3D-Nf surfaces with a suitable solvent has highly significant effects on morphology, and ultimately, electrochemical performance. Additionally, the current preparation approach is simple and worthwhile, and thus offers great potential for supercapacitor applications.
Using Twitter to Detect Hate Crimes and Their Motivations: The HateMotiv Corpus
Authors: Alnazzawi Noha
Published: May 2022

With the rapidly increasing use of social media platforms, much of our lives is spent online. Despite the great advantages of using social media, unfortunately, the spread of hate, cyberbullying, harassment, and trolling can be very common online. Many extremists use social media platforms to communicate their messages of hatred and spread violence, which may result in serious psychological consequences and even contribute to real-world violence. Thus, the aim of this research was to build the HateMotiv corpus, a freely available dataset that is annotated for types of hate crimes and the motivation behind committing them. The dataset was developed using Twitter as an example of social media platforms and could provide the research community with a very unique, novel, and reliable dataset. The dataset is unique as a consequence of its topic-specific nature and its detailed annotation. The corpus was annotated by two annotators who are experts in annotation based on unified guidelines, so they were able to produce an annotation of a high standard with F-scores for the agreement rate as high as 0.66 and 0.71 for type and motivation labels of hate crimes, respectively.
Predictors of Bone Metastases at 68Ga-PSMA-11 PET/CT in Hormone-Sensitive Prostate Cancer (HSPC) Patients with Early Biochemical Recurrence or Persistence
Authors: Rovera Guido , Grimaldi Serena , Dall’Armellina Sara , Passera Roberto , Oderda Marco , Iorio Giuseppe Carlo , Guarneri Alessia , Gontero Paolo , Ricardi Umberto , Deandreis Désirée
Published: May 2022

Prostate-specific-membrane-antigen/positron-emission-tomography (PSMA-PET) can accurately detect disease localizations in prostate cancer (PCa) patients with early biochemical recurrence/persistence (BCR/BCP), allowing for more personalized image-guided treatments in oligometastatic patients with major impact in the case of bone metastases (BM). Therefore, this study aimed to identify predictors of BM at PSMA-PET in early-BCR/BCP hormone-sensitive PCa (HSPC) patients, previously treated with radical intent (radiotherapy or radical prostatectomy ± salvage-radiotherapy (SRT)). A retrospective analysis was performed on 443 68Ga-PSMA-11-PET/CT scans. The cohort median PSA at PET-scan was 0.60 (IQR: 0.38–1.04) ng/mL. PSMA-PET detection rate was 42.0% (186/443), and distant lesions (M1a/b/c) were found in 17.6% (78/443) of cases. BM (M1b) were present in 9.9% (44/443) of cases, with 70.5% (31/44) showing oligometastatic spread (≤3 PSMA-positive lesions). In the multivariate binary logistic regression model (accuracy: 71.2%, Nagelkerke-R2: 13%), T stage ≥ 3a (OR: 2.52; 95% CI: 1.13–5.60; p = 0.024), clinical setting (previous SRT vs. first-time BCR OR: 2.90; 95% CI: 1.32–6.35; p = 0.008), and PSAdt (OR: 0.93; 95% CI: 0.88–0.99; p = 0.026) were proven to be significant predictors of bone metastases, with a 7% risk increment for each single-unit decrement of PSAdt. These predictors could be used to further refine the indication for PSMA-PET in early BCR/BCP HSPC patients, leading to higher detection rates of bone disease and more personalized treatments.