bluuebunny/crossref_metadata_2025 huggingface dataset:
Wearable Sensors for Biochemical Sweat Analysis
Authors: Bandodkar Amay J. , Jeang William J. , Ghaffari Roozbeh , Rogers John A.
Published: February 2019

Sweat is a largely unexplored biofluid that contains many important biomarkers ranging from electrolytes and metabolites to proteins, cytokines, antigens, and exogenous drugs. The eccrine and apocrine glands produce and excrete sweat through microscale pores on the epidermal surface, offering a noninvasive means for capturing and probing biomarkers that reflect hydration state, fatigue, nutrition, and physiological changes. Recent advances in skin-interfaced wearable sensors capable of real-time in situ sweat collection and analytics provide capabilities for continuous biochemical monitoring in an ambulatory mode of operation. This review presents a broad overview of sweat-based biochemical sensor technologies with an emphasis on enabling materials, designs, and target analytes of interest. The article concludes with a summary of challenges and opportunities for researchers and clinicians in this swiftly growing field.
Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors
Authors: Yeh Hsien-Wei , Ai Hui-Wang
Published: February 2019

Although fluorescent reporters and biosensors have become indispensable tools in biological and biomedical fields, fluorescence measurements require external excitation light, thereby limiting their use in thick tissues and live animals. Bioluminescent reporters and biosensors may potentially overcome this hurdle because they use enzyme-catalyzed exothermic biochemical reactions to generate excited-state emitters. This review first introduces the development of bioluminescent reporters, and next, their applications in sensing biological changes in vitro and in vivo as biosensors. Lastly, we discuss chemiluminescent sensors that produce photons in the absence of luciferases. This review aims to explore fundamentals and experimental insights and to emphasize the yet-to-be-reached potential of next-generation luminescent reporters and biosensors.
Thermomorphogenesis
Authors: Casal Jorge J. , Balasubramanian Sureshkumar
Published: February 2019

When exposed to warmer, nonstressful average temperatures, some plant organs grow and develop at a faster rate without affecting their final dimensions. Other plant organs show specific changes in morphology or development in a response termed thermomorphogenesis. Selected coding and noncoding RNA, chromatin features, alternative splicing variants, and signaling proteins change their abundance, localization, and/or intrinsic activity to mediate thermomorphogenesis. Temperature, light, and circadian clock cues are integrated to impinge on the level or signaling of hormones such as auxin, brassinosteroids, and gibberellins. The light receptor phytochrome B (phyB) is a temperature sensor, and the phyB–PHYTOCHROME-INTERACTING FACTOR 4 (PIF4)–auxin module is only one thread in a complex network that governs temperature sensitivity. Thermomorphogenesis offers an avenue to search for climate-smart plants to sustain crop and pasture productivity in the context of global climate change.
A Molecular View of Plant Local Adaptation: Incorporating Stress-Response Networks
Authors: VanWallendael Acer , Soltani Ali , Emery Nathan C. , Peixoto Murilo M. , Olsen Jason , Lowry David B.
Published: February 2019

Ecological specialization in plants occurs primarily through local adaptation to different environments. Local adaptation is widely thought to result in costly fitness trade-offs that result in maladaptation to alternative environments. However, recent studies suggest that such trade-offs are not universal. Further, there is currently a limited understanding of the molecular mechanisms responsible for fitness trade-offs associated with adaptation. Here, we review the literature on stress responses in plants to identify potential mechanisms underlying local adaptation and ecological specialization. We focus on drought, high and low temperature, flooding, herbivore, and pathogen stresses. We then synthesize our findings with recent advances in the local adaptation and plant molecular biology literature. In the process, we identify mechanisms that could cause fitness trade-offs and outline scenarios where trade-offs are not a necessary consequence of adaptation. Future studies should aim to explicitly integrate molecular mechanisms into studies of local adaptation.
Molecular Mechanisms of Plant Regeneration
Authors: Ikeuchi Momoko , Favero David S. , Sakamoto Yuki , Iwase Akira , Coleman Duncan , Rymen Bart , Sugimoto Keiko
Published: February 2019

Plants reprogram somatic cells following injury and regenerate new tissues and organs. Upon perception of inductive cues, somatic cells often dedifferentiate, proliferate, and acquire new fates to repair damaged tissues or develop new organs from wound sites. Wound stress activates transcriptional cascades to promote cell fate reprogramming and initiate new developmental programs. Wounding also modulates endogenous hormonal responses by triggering their biosynthesis and/or directional transport. Auxin and cytokinin play pivotal roles in determining cell fates in regenerating tissues and organs. Exogenous application of these plant hormones enhances regenerative responses in vitro by facilitating the activation of specific developmental programs. Many reprogramming regulators are epigenetically silenced during normal development but are activated by wound stress and/or hormonal cues.
Prevention of Opioid Abuse and Treatment of Opioid Addiction: Current Status and Future Possibilities
Authors: Park Kinam , Otte Andrew
Published: February 2019

Prescription opioid medications have seen a dramatic rise in misuse and abuse, leading regulators and scientists to develop policies and abuse-deterrent technologies to combat the current opioid epidemic. These abuse-deterrent formulations (ADFs) are intended to deter physical and chemical tampering of opioid-based products, while still providing safe and effective delivery for therapeutic purposes. Even though formulations with varying abuse-deterrent technologies have been approved, questions remain about their effectiveness. While these formulations provide a single means to combat the epidemic, a greater emphasis should be placed on formulations for treatment of addiction and overdose to help those struggling with opioid dependence. This article analyzes various ADFs currently in clinical use and explores potential novel systems for treatment of addiction and prevention of overdose.
Split Green Fluorescent Proteins: Scope, Limitations, and Outlook
Authors: Romei Matthew G. , Boxer Steven G.
Published: February 2019

Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein–protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies.
Mammalian Respiratory Complex I Through the Lens of Cryo-EM
Authors: Agip Ahmed-Noor A. , Blaza James N. , Fedor Justin G. , Hirst Judy
Published: February 2019

Single-particle electron cryomicroscopy (cryo-EM) has led to a revolution in structural work on mammalian respiratory complex I. Complex I (mitochondrial NADH:ubiquinone oxidoreductase), a membrane-bound redox-driven proton pump, is one of the largest and most complicated enzymes in the mammalian cell. Rapid progress, following the first 5-Å resolution data on bovine complex I in 2014, has led to a model for mouse complex I at 3.3-Å resolution that contains 96% of the 8,518 residues and to the identification of different particle classes, some of which are assigned to biochemically defined states. Factors that helped improve resolution, including improvements to biochemistry, cryo-EM grid preparation, data collection strategy, and image processing, are discussed. Together with recent structural data from an ancient relative, membrane-bound hydrogenase, cryo-EM on mammalian complex I has provided new insights into the proton-pumping machinery and a foundation for understanding the enzyme's catalytic mechanism.
History of Psychopharmacology
Authors: Braslow Joel T. , Marder Stephen R.
Published: February 2019

We live in an age of psychopharmacology. One in six persons currently takes a psychotropic drug. These drugs have profoundly shaped our scientific and cultural understanding of psychiatric disease. By way of a historical review, we try to make sense of psychiatry's dependency on psychiatric drugs in the care of patients. Modern psychopharmacology began in 1950 with the synthesis of chlorpromazine. Over the course of the next 50 years, the psychiatric understanding and treatment of mental illness radically changed. Psychotropic drugs played a major part in these changes as state hospitals closed and psychotherapy gave way to drug prescriptions. Our review suggests that the success of psychopharmacology was not the consequence of increasingly more effective drugs for discrete psychiatric diseases. Instead, a complex mix of political economic realities, pharmaceutical marketing, basic science advances, and changes in the mental health-care system have led to our current infatuation with psychopharmacology.
Estrogen, Stress, and Depression: Cognitive and Biological Interactions
Authors: Albert Kimberly M. , Newhouse Paul A.
Published: February 2019

This article reviews the interactions of estrogen changes and psychosocial stress in contributing to vulnerability to major depressive disorder (MDD) in women. Estrogen modulates brain networks and processes related to changes in stress response, cognition, and emotional dysregulation that are core characteristics of MDD. Synergistic effects of estrogen on cognitive and emotional function, particularly during psychosocial stress, may underlie the association of ovarian hormone fluctuation and depression in women. We propose a model of estrogen effects on multiple brain systems that interface with stress-related emotional and cognitive processes implicated in MDD and discuss possible mechanisms through which reproductive events and changes in estrogen may contribute to MDD risk in women with other concurrent risk factors.
Using a Developmental Ecology Framework to Align Fear Neurobiology Across Species
Authors: Callaghan Bridget , Meyer Heidi , Opendak Maya , Van Tieghem Michelle , Harmon Chelsea , Li Anfei , Lee Francis S. , Sullivan Regina M. , Tottenham Nim
Published: February 2019

Children's development is largely dependent on caregiving; when caregiving is disrupted, children are at increased risk for numerous poor outcomes, in particular psychopathology. Therefore, determining how caregivers regulate children's affective neurobiology is essential for understanding psychopathology etiology and prevention. Much of the research on affective functioning uses fear learning to map maturation trajectories, with both rodent and human studies contributing knowledge. Nonetheless, as no standard framework exists through which to interpret developmental effects across species, research often remains siloed, thus contributing to the current therapeutic impasse. Here, we propose a developmental ecology framework that attempts to understand fear in the ecological context of the child: their relationship with their parent. By referring to developmental goals that are shared across species (to attach to, then, ultimately, separate from the parent), this framework provides a common grounding from which fear systems and their dysfunction can be understood, thus advancing research on psychopathologies and their treatment.
Abnormal Sleep Spindles, Memory Consolidation, and Schizophrenia
Authors: Manoach Dara S. , Stickgold Robert
Published: February 2019

There is overwhelming evidence that sleep is crucial for memory consolidation. Patients with schizophrenia and their unaffected relatives have a specific deficit in sleep spindles, a defining oscillation of non-rapid eye movement (NREM) Stage 2 sleep that, in coordination with other NREM oscillations, mediate memory consolidation. In schizophrenia, the spindle deficit correlates with impaired sleep-dependent memory consolidation, positive symptoms, and abnormal thalamocortical connectivity. These relations point to dysfunction of the thalamic reticular nucleus (TRN), which generates spindles, gates the relay of sensory information to the cortex, and modulates thalamocortical communication. Genetic studies are beginning to provide clues to possible neurodevelopmental origins of TRN-mediated thalamocortical circuit dysfunction and to identify novel targets for treating the related memory deficits and symptoms. By forging empirical links in causal chains from risk genes to thalamocortical circuit dysfunction, spindle deficits, memory impairment, symptoms, and diagnosis, future research can advance our mechanistic understanding, treatment, and prevention of schizophrenia.
Topological Semimetals in Square-Net Materials
Authors: Klemenz Sebastian , Lei Shiming , Schoop Leslie M.
Published: February 2019

Many materials crystallize in structure types that feature a square net of atoms. While these compounds can exhibit many different properties, some members of this family are topological materials. Within the square-net-based topological materials, the observed properties are rich, ranging, for example, from nodal-line semimetals to a bulk half-integer quantum Hall effect. Hence, the potential for guided design of topological properties is enormous. Here we provide an overview of the crystallographic and electronic properties of these phases and show how they are linked, with the goal of understanding which square-net materials can be topological, and predict additional examples. We close the review by discussing the experimentally observed electronic properties in this family.
Mechanical Control of Magnetic Order: From Phase Transition to Skyrmions
Authors: Wang Jie
Published: February 2019

Topological magnetic structures such as domain walls, vortices, and skyrmions have recently received considerable attention because of their potential application in advanced functional devices. Tuning the magnetic order of the topological structures can result in emergent functionalities and thus lead to novel application concepts. Strain engineering is one promising approach with which to control magnetic order via magneto-elastic coupling in ferromagnets. By introducing lattice deformation, mechanical strain not only can trigger the magnetic phase transition but also can be used to manipulate topological magnetic orders in ferromagnets. The present review is based on magneto-elastic coupling as the coherent basis of the mechanical control of different topological magnetic orders. Following a description of magneto-elastic coupling, we review recent progress in the mechanical control of the magnetic phase transition and topological structures, including magnetic domain walls, vortices, and skyrmions. The review concludes by briefly addressing the future research directions in the field.
Better Government, Better Science: The Promise of and Challenges Facing the Evidence-Informed Policy Movement
Authors: Bowers Jake , Testa Paul F.
Published: February 2019

Collaborations between the academy and governments promise to improve the lives of people, the operations of government, and our understanding of human behavior and public policy. This review shows that the evidence-informed policy movement consists of two main threads: ( a) an effort to invent new policies using insights from the social and behavioral science consensus about human behavior and institutions and ( b) an effort to evaluate the success of governmental policies using transparent and high-integrity research designs such as randomized controlled trials. We argue that the problems of each approach may be solved or at least well addressed by teams that combine the two. We also suggest that governmental actors ought to want to learn about why a new policy works as much as they want to know that the policy works. We envision a future evidence-informed public policy practice that ( a) involves cross-sector collaborations using the latest theory plus deep contextual knowledge to design new policies, ( b) applies the latest insights in research design and statistical inference for causal questions, and ( c) is focused on assessing explanations as much as on discovering what works. The evidence-informed public policy movement is a way that new data, new questions, and new collaborators can help political scientists improve our theoretical understanding of politics and also help our policy partners to improve the practice of government itself.
Physiological effects of altering oxygenation during kidney normothermic machine perfusion
Authors: Adams Thomas D. , Hosgood Sarah A. , Nicholson Michael L.
Published: February 2019

Kidney normothermic machine perfusion (NMP) has historically used a 95% O2-5% CO2 gas mixture. Using a porcine model of organ retrieval, NMP, and reperfusion, we tested the hypothesis that reducing perfusate oxygenation ([Formula: see text]) would be detrimental to renal function and cause injury. In the minimal ischemic injury experiment, kidneys sustained 10 min of warm ischemia and 2 h of static cold storage before 1 h of NMP with either 95%, 25%, or 12% O2 with 5% CO2 and N2 balance. In the clinical injury experiment, kidneys with 10-min warm ischemia and 17-h static cold storage underwent 1-h NMP with the above gas combinations or 18-h static cold storage as a control. They were then reperfused with whole blood and 95% O2 for 3 h. Overall, reducing [Formula: see text] did not significantly influence renal function in either experiment. Furthermore, there were no differences in the injury markers urinary neutrophil gelatinase-associated lipocalin or tissue high-motility group box protein 1. In the minimal ischemic injury experiment, a [Formula: see text] of 25% significantly reduced renal blood flow and increased vascular resistance. Oxygen delivery, consumption, and extraction (oxygen extraction ratio) were significantly greater at 95% [Formula: see text]. In the clinical injury experiment, renal blood flow was significantly increased at 25% [Formula: see text] and Na+ excretion decreased. At 95% [Formula: see text], the oxygen content and oxygen extraction ratio were significantly increased. During reperfusion, renal blood flow was significantly increased in the 25% group. The control group pH was significantly decreased compared with the 25% group. Our data suggest that reducing [Formula: see text] during NMP does not have detrimental effects on renal function or markers of injury.
Processing of fast amplitude modulations in bat auditory cortex matches communication call-specific sound features
Authors: Hörpel Stephen Gareth , Firzlaff Uwe
Published: February 2019

Bats use a large repertoire of calls for social communication. In the bat Phyllostomus discolor, social communication calls are often characterized by sinusoidal amplitude and frequency modulations with modulation frequencies in the range of 100–130 Hz. However, peaks in mammalian auditory cortical modulation transfer functions are typically limited to modulation frequencies below 100 Hz. We investigated the coding of sinusoidally amplitude modulated sounds in auditory cortical neurons in P. discolor by constructing rate and temporal modulation transfer functions. Neuronal responses to playbacks of various communication calls were additionally recorded and compared with the neurons’ responses to sinusoidally amplitude-modulated sounds. Cortical neurons in the posterior dorsal field of the auditory cortex were tuned to unusually high modulation frequencies: rate modulation transfer functions often peaked around 130 Hz (median: 87 Hz), and the median of the highest modulation frequency that evoked significant phase-locking was also 130 Hz. Both values are much higher than reported from the auditory cortex of other mammals, with more than 51% of the units preferring modulation frequencies exceeding 100 Hz. Conspicuously, the fast modulations preferred by the neurons match the fast amplitude and frequency modulations of prosocial, and mostly of aggressive, communication calls in P. discolor. We suggest that the preference for fast amplitude modulations in the P. discolor dorsal auditory cortex serves to reliably encode the fast modulations seen in their communication calls.

NEW & NOTEWORTHY Neural processing of temporal sound features is crucial for the analysis of communication calls. In bats, these calls are often characterized by fast temporal envelope modulations. Because auditory cortex neurons typically encode only low modulation frequencies, it is unclear how species-specific vocalizations are cortically processed. We show that auditory cortex neurons in the bat Phyllostomus discolor encode fast temporal envelope modulations. This property improves response specificity to communication calls and thus might support species-specific communication.
Serum Thyroglobulin: Preoperative Levels and Factors Affecting Postoperative Optimal Timing following Total Thyroidectomy
Authors: Patel Anery , Shostrom Valerie , Treude Kelly , Lydiatt William , Smith Russell , Goldner Whitney
Published: February 2019

Objective. We evaluated if preoperative TG levels affected postoperative levels and if other factors may influence the optimal time to check postoperative TG. Methods. This is a prospective, observational pilot study. We approved and enrolled 50 subjects≥19 years scheduled for total thyroidectomy and measured serum TG, thyroglobulin antibody (TG ab), and TSH preoperatively and post thyroidectomy at 7-14 days, 4 and 6 weeks, and 3 months in subjects with benign pathology, with additional 6- and 12-month measurements in subjects with thyroid cancer. Results. Preoperative TG was significantly higher in the benign (median 167.5 ng/mL vs 30.8 ng/mL) than in the malignant (p=0.0006) group. In the benign group, 76.5% (13/17) of subjects had an undetectable TG<0.2 ng/mL by 12 weeks postoperatively. In the malignant group, 70.6% (12/17) of those who did not receive RAI therapy and 25% (1/4) of those who did receive RAI had undetectable TG<0.2 ng/mL by 12 weeks. Subset analysis showed 94.1% (16/17) of the benign, 70.6% of the malignant without RAI, and 50% (2/4) of the malignant with RAI achieved TG<1.0 ng/mL by 6 weeks postoperatively. Four subjects in the malignant group reached undetectable TG levels as early as 7-14 days postoperatively. Conclusion. Preoperative TG levels did not predict the risk of malignancy nor time to TG nadir postoperatively. We did not find a difference in TG elimination half-life between the benign and malignant groups. The median time to reach undetectable TG levels in both benign and malignant groups who did not receive RAI therapy was 12 weeks. However, those with preexisting hypothyroidism and hyperthyroidism had lower levels of TG overall in the malignant group which can be taken into consideration besides other known factors that can affect TG levels post thyroidectomy. This trial is registered with Clinicaltrials.gov NCT02347683.
Anti-Inflammatory Activities of Leaf Oil from Cinnamomum subavenium In Vitro and In Vivo
Authors: Hao Xincai , Sun Weiguang , Ke Changbin , Wang Fuqian , Xue Yongbo , Luo Zengwei , Wang Xuanbin , Zhang Jinwen , Zhang Yonghui
Published: February 2019

The study determined the chemical constituents and anti-inflammatory effects of leaf oil from Cinnamomum subavenium (CS-LO) that has been used in folk medicine to treat various symptoms including inflammation. The anti-inflammatory effects of the oil were evaluated by LPS-stimulated RAW264.7 cells and the Carr-induced hind mouse paw edema model, respectively. In vitro, nitric oxide (NO), prostaglandin E2 (PGE2), TNF-α, IL-6, and IL-1β were significantly decreased by CS-LO, and the expression of nuclear factor-κB (NF-κB) protein was blocked as well. In in vivo, the malondialdehyde (MDA) and paw edema levels were decreased by CS-LO, and the same result came up on the NO and tumor necrosis factor (TNF-a) of serum at the 5th h after Carr injection. In addition, iNOS and COX-2 immunoreactive cells of the paw tissue were decreased significantly by CS-LO (200 mg/kg) in histological examination. The present findings indicated that CS-LO have anti-inflammatory properties, and the effects might be caused through inhibiting iNOS, COX-2, TNF-α, IL-1β, and IL-6 expression via affecting NF-κB pathway, which will provide a power scientific basis for CS-LO to be used as the treatment of inflammatory diseases.
Comparative Evaluation of Plaque Inhibitory and Antimicrobial Efficacy of Probiotic and Chlorhexidine Oral Rinses in Orthodontic Patients: A Randomized Clinical Trial
Authors: Shah Shreya Shruti , Nambiar Supriya , Kamath Deepa , Suman Ethel , Unnikrishnan Bhaskaran , Desai Asavari , Mahajan Sanchit , Dhawan Kushan Kishore
Published: February 2019

Background. Antimicrobial agents serve as an effective adjunct in plaque control, and chlorhexidine has been the gold standard. However, the philosophy that natural agents are better for children's oral health is on the rise. Probiotic technology represents a breakthrough approach to maintain oral health by utilizing natural beneficial bacteria commonly found in healthy mouths. Aim and Objective. To compare efficacy of probiotic and chlorhexidine oral rinses in orthodontic patients. Materials and Methods. 30 healthy patients undergoing fixed orthodontic treatment were randomly selected for the study by block randomization and allocation concealment and were divided into three groups: group a, 0.2% chlorhexidine mouthwash; group b, probiotic mouthwash; and group c, a control group. Results. Probiotic and chlorhexidine groups had significantly decreased plaque indices as compared to the control group. However, greater improvement was seen in the gingival indices than plaque indices with better results in the probiotic group than the chlorhexidine group. No statistical significance was observed in the streptococcus count of probiotic and chlorhexidine groups at the end of the intervention period. Conclusion. The comparison of probiotics to chlorhexidine has proven that probiotics are as effective as chlorhexidine as an adjunctive chemical plaque control agent.
Tankyrase Promotes Aerobic Glycolysis and Proliferation of Ovarian Cancer through Activation of Wnt/β-Catenin Signaling
Authors: Yang Hong-Yi , Shen Jin-Xing , Wang Yi , Liu Yu , Shen Dong-Yan , Quan Song
Published: February 2019

Tankyrase (TNKS) plays important roles in the malignancy of several cancers such as human lung tumor, breast cancer, and hepatocellular cancer. However, its exact functions and molecular mechanisms in ovarian cancer remain unclear. In this study, we found that TNKS was aberrantly overexpressed in human ovarian cancer tissues and associated with poor patient prognosis. TNKS inhibition or knockdown not only reduced ovarian cancer cell proliferation, colony formation, migration, invasion, and tumorigenic potential in nude mice but also enhanced the drug susceptibility of ovarian cancer cells through arresting cell cycle and inducing apoptosis. These phenotypic changes correlated with downregulation of targets (Cyclin D1, MDR, and MMP-9) of Wnt/β-catenin signaling. Furthermore, downregulation of TNKS suppressed the glucose uptake, lactate excretion, and cellular ATP levels and increased cellular O2consumption rates. Molecular mechanism studies revealed that TNKS promoted aerobic glycolysis at least in part due to upregulation of pyruvate carboxylase (PC) via activation of Wnt/β-catenin/snail signaling. In agreement with these findings, expression of TNKS is positively associated with snail and PC in clinical ovarian cancer samples. Our findings identified TNKS as an oncogenic regulator of ovarian cancer cells proliferation that promotes aerobic glycolysis via activation of Wnt/β-catenin signaling, indicating that the TNKS might serve as a potential molecular target for clinical therapy of Wnt/β-catenin dependent ovarian cancer.
Experimental Study on Upper-Limb Rehabilitation Training of Stroke Patients Based on Adaptive Task Level: A Preliminary Study
Authors: Pan Lizheng , Song Aiguo , Wang Simei , Duan Suolin
Published: February 2019

During robot-aided motion rehabilitation training, inappropriate difficulty of the training task usually leads the subject becoming bored or frustrated; therefore, the difficulty of the training task has an important influence on the effectiveness of training. In this study, an adaptive task level strategy is proposed to intelligently serve the subject with a task of suitable difficulty. To make the training task attractive and continuously stimulate the patient's training enthusiasm, diverse training tasks based on grabbing game with visual feedback are developed. Meanwhile, to further enhance training awareness and inculcate a sense of urgency, a dynamic score feedback method is used in the design of the training tasks. Two types of experiments, functional and clinical rehabilitation experiments, were performed with a healthy adult and two recruited stroke patients, respectively. The experimental results suggest that the proposed adaptive task level strategy and dynamic score feedback method are effective strategies with respect to incentive function and rehabilitation efficacy.
Using Kinect v2 to Control a Laser Visual Cue System to Improve the Mobility during Freezing of Gait in Parkinson’s Disease
Authors: Amini Amin , Banitsas Konstantinos
Published: February 2019

Different auditory and visual cues have been proven to be very effective in improving the mobility of people with Parkinson’s (PwP). Nonetheless, many of the available methods require user intervention and so on to activate the cues. Moreover, once activated, these systems would provide cues continuously regardless of the patient’s needs. This research proposes a new indoor method for casting dynamic/automatic visual cues for PwP based on their head direction and location in a room. The proposed system controls the behavior of a set of pan/tilt servo motors and laser pointers, based on the real-time skeletal information acquired from a Kinect v2 sensor. This produces an automatically adjusting set of laser lines that can always be in front of the patient as a guideline for where the next footstep would be placed. A user interface was also created that enables users to control and adjust the settings based on the preferences. The aim of this research was to provide PwP with an unobtrusive/automatic indoor system for improving their mobility during a Freezing of gait (FOG) incident. The results showed the possibility of employing such system, which does not rely on the subject’s input nor does it introduce any additional complexities to operate.
Multifocus Image Fusion Using Wavelet-Domain-Based Deep CNN
Authors: Li Jinjiang , Yuan Genji , Fan Hui
Published: February 2019

Multifocus image fusion is the merging of images of the same scene and having multiple different foci into one all-focus image. Most existing fusion algorithms extract high-frequency information by designing local filters and then adopt different fusion rules to obtain the fused images. In this paper, a wavelet is used for multiscale decomposition of the source and fusion images to obtain high-frequency and low-frequency images. To obtain clearer and complete fusion images, this paper uses a deep convolutional neural network to learn the direct mapping between the high-frequency and low-frequency images of the source and fusion images. In this paper, high-frequency and low-frequency images are used to train two convolutional networks to encode the high-frequency and low-frequency images of the source and fusion images. The experimental results show that the method proposed in this paper can obtain a satisfactory fusion image, which is superior to that obtained by some advanced image fusion algorithms in terms of both visual and objective evaluations.
Extreme Oncoplastic Surgery for Multifocal/Multicentric and Locally Advanced Breast Cancer
Authors: Koppiker Chaitanyanand B. , Noor Aijaz Ul , Dixit Santosh , Busheri Laleh , Sharan Gautam , Dhar Upendra , Allampati Hari Kiran , Nare Smeeta
Published: February 2019

Introduction. Breast conserving surgery (BCS) followed by radiation therapy (RT) has become the preferred alternative to mastectomy for patients with early stage breast cancer (BC). Randomized trials have confirmed equivalent locoregional control and overall survival for BCS and mastectomy. Extreme Oncoplasty (EO) extends the indications of BCS for patients who would otherwise require mastectomy, ensuring better aesthetic outcomes and oncological safety. Methods. BC patients with multifocal/multicentric (MF/MC) tumors, extensive DCIS, or large tumor >50mm underwent EO at our breast unit. Therapeutic reduction mammaplasty (TRM) with wise pattern preoperative markings and dual pedicle technique involving parenchymal rearrangement was used for oncoplastic reconstructions in majority of the cases followed by RT. Patient reported outcome measures (PROMs) were assessed using the validated Breast-Q questionnaire. Results. Of the 39 patients in the study, 36 had unilateral and 3 had bilateral BC. Mean age was 47.2 years. Median tumor size was 75mm. 17 (43.6%) patients received NACT; none achieved a complete clinical response. 28 (71.8%) patients were administered to adjuvant chemotherapy. 33(84.6%) patients received RT to the breast with a median dose of 50Gy in 28 fractions and a boost dose of 10Gy in 5 fractions to the tumor bed. No major complications or local recurrences were observed. Excellent Breast-Q scores were observed in patients undergoing EO after 12 months of follow-up. Conclusion. EO followed by RT results in acceptable local-regional control, low rate of complications, and high patient satisfaction. In selected patients, EO could provide a safe alternative for breast conservation surgery instead of mastectomy.
Ubenimex Reverses MDR in Gastric Cancer Cells by Activating Caspase-3-Mediated Apoptosis and Suppressing the Expression of Membrane Transport Proteins
Authors: Guo Qie , Jing Fan-jing , Qu Hai-jun , Xu Wen , Han Bing , Xing Xiao-min , Ji Hong-yan , Jing Fan-Bo
Published: February 2019

Gastric cancer (GC) is one of the most malignant tumors, accounting for 10% of deaths caused by all cancers. Chemotherapy is often necessary for treatment of GC; the FOLFOX regimen is extensively applied. However, multidrug resistance (MDR) of GC cells prevents wider application of this treatment. Ubenimex, an inhibitor of CD13, is used as an immune adjuvant to treat hematological malignancies. Here, we demonstrate that CD13 expression positively correlates with MDR development in GC cells. Moreover, Ubenimex reverses the MDR of SGC7901/X and MKN45/X cells and enhances their sensitivity to FOLFOX, in part by decreasing CD13 expression, which is accompanied by downregulation of Bcl-xl, Bcl-2, and survivin expression; increased expression of Bax; and activation of the caspase-3-mediated apoptotic cascade. In addition, Ubenimex downregulates expression of membrane transport proteins, such as P-gp and MRP1, by inhibiting phosphorylation in the PI3K/AKT/mTOR pathway to increase intracellular accumulations of 5-fluorouracil and oxaliplatin, a process for which downregulation of CD13 expression is essential. Therefore, the present results reveal a previously uncharacterized function of CD13 in promoting MDR development in GC cells and suggest that Ubenimex is a candidate for reversing the MDR of GC cells.
An NFV-Based Energy Scheduling Algorithm for a 5G Enabled Fleet of Programmable Unmanned Aerial Vehicles
Authors: Tipantuña Christian , Hesselbach Xavier , Sánchez-Aguero Victor , Valera Francisco , Vidal Ivan , Nogales Borja
Published: February 2019

The fifth generation of mobile networks (5G) is expected to provide diverse and stringent improvements such as greater connectivity, bandwidth, throughput, availability, improved coverage, and lower latency. Considering this, drones or Unmanned Aerial Vehicles (UAVs) and Internet of Things (IoT) devices are perfect examples of existing technology that can take advantage of the capabilities provided by 5G technology. In particular, UAVs are expected to be an important component of 5G networks implementations and support different communication requirements and applications. UAVs working together with 5G can potentially facilitate the deployment of standalone or complementary communications infrastructures, and, due to its rapid deployment, these solutions are suitable candidates to provide network services in emergency scenarios, natural disasters, and search and rescue missions. An important consideration in the deployment of a programmable drone fleet is to guarantee the reliability and performance of the services through consistent monitoring, control, and management scheme. In this regard, the Network Functions Virtualization (NFV) paradigm, a key technology within the 5G ecosystem, can be used to perform automation, management, and orchestration tasks. In addition, to ensure the coordination and reliability in the communications systems, considering that the UAVs have a finite lifetime and that eventually they must be replaced, a scheduling scheme is needed to guarantee the availability of services and efficient resource utilization. To this end, in this paper is presented an UAV scheduling scheme which leverages the potential offered by NFV. The proposed strategy, based on a brute-force search combinatorial algorithm, allows obtaining the optimal scheduling of UAVs in time, in order to efficiently deploy network services. Simulation results validate the performance of the proposed strategy, by providing the number of drones needed to meet certain levels of service availability. Furthermore, the strategy allows knowing the sequence of replacement of UAVs to ensure the optimal resource utilization.
Transient Global Amnesia as the Sole Presentation of an Acute Stroke in the Left Cingulate Gyrus
Authors: Chau Lawrence , Liu Antonio
Published: February 2019

We discuss a patient who presented to our hospital with signs, symptoms, and clinical course consistent with transient global amnesia (TGA). Her hospital work-up was overall unremarkable except for the presence of a diffusion weighted imaging (DWI) abnormality on MRI suggestive of acute infarction in the left cingulate gyrus. Symptoms quickly resolved, and she was discharged home in stable condition. While the etiology of TGA remains controversial, in discussing this case and the current literature we hope to provide further data supporting the possibility of an underlying ischemic process, as well as help better illustrate the neurologic structures involved.
Practical Web Spam Lifelong Machine Learning System with Automatic Adjustment to Current Lifecycle Phase
Authors: Luckner Marcin
Published: February 2019

Machine learning techniques are a standard approach in spam detection. Their quality depends on the quality of the learning set, and when the set is out of date, the quality of classification falls rapidly. The most popular public web spam dataset that can be used to train a spam detector—WEBSPAM-UK2007—is over ten years old. Therefore, there is a place for a lifelong machine learning system that can replace the detectors based on a static learning set. In this paper, we propose a novel web spam recognition system. The system automatically rebuilds the learning set to avoid classification based on outdated data. Using a built-in automatic selection of the active classifier the system very quickly attains productive accuracy despite a limited learning set. Moreover, the system automatically rebuilds the learning set using external data from spam traps and popular web services. A test on real data from Quora, Reddit, and Stack Overflow proved the high recognition quality. Both the obtained average accuracy and the F-measure were 0.98 and 0.96 for semiautomatic and full–automatic mode, respectively.
C18H17NO6and Its Combination with Scutellarin Suppress the Proliferation and Induce the Apoptosis of Human Glioma Cells via Upregulation of Fas-Associated Factor 1 Expression
Authors: He Xiu-Ying , Xiong Liu-Lin , Xia Qing-Jie , Wang Yang-Yang , Zhao Xiao-Ming , Du Ruo-Lan , Huang Jin , He Xiao-Qiong , Jia-Liu  , Wang Ting-Hua
Published: February 2019

Background. Glioma is the most common malignant brain tumor and the patients are prone to poor prognosis. Due to limited treatments, new drug exploration has become a general trend. Therefore, the objective of this study is to investigate the effect of the new drugs C18H17NO6and its combination with Scutellarin on glioma cells and the underlying mechanism.Method. U251 and LN229 cells were administrated with C18H17NO6and its combination with Scutellarin. The proliferation ability of glioma cells was determined by cell counting kit-8, plate clone formation assay, and EdU incorporation assay. The cell cycle and apoptosis detection were detected by flow cytometry. Moreover, TUNEL assay was also used for cell apoptosis analysis. Then, the transfer ability of cells was achieved through wound healing assay. Furthermore, polymerase chain reaction (PCR) test and western bolt analysis were used to detect the mRNA expression and protein expression, respectively. Lastly, immunofluorescence was for the purity identification of astrocyte.Result. The results showed that, with the increasing dose of C18H17NO6, the cell inhibition rate, the cells in G1 phase, and the apoptosis rate were gradually increased, but the clone number, proliferation rate, and the cells in G2 and S phases were gradually decreased in comparison with control group. However, with the increase of C18H17NO6, the transferred rate of U251 and LN229 was not significantly augmented, expect that on U251 in C18H17NO65μM group. In addition, Scutellarin 200μM has little effect on proliferation, with the inhibition rate 10-20% and proliferation rate except U251 in Scutellarin 200μM group similar to that in control group. Moreover, compared to control group, Scutellarin 300μM increased the U251 cells in G2 and S phases and the apoptosis rate of LN229 but decreased the LN229 cells in G2 and S phases. Besides, in Scutellarin 200μM group, the transfer ability of LN229 was inhibited, but not in U251. Furthermore, if C18H17NO6was combined with Scutellarin 200/300μM, the proliferation and transferred ability were suppressed and the apoptosis was elevated in LN229 cell in comparison with C18H17NO6alone. Dramatically, the combined effect on U251 was the exact opposite. Importantly, there was little toxicity on astrocyte under the dose of C18H17NO6and Scutellarin in the study. In molecular level, the mRNA and protein expression of Fas-associated factor 1 (FAF1) expression in U251 and LN229 were upregulated by C18H17NO6and its combination with Scutellarin, especially the protein expression.Conclusion. C18H17NO6could efficiently suppress cell proliferation and induce cell apoptosis in glioma cells, and its combination with Scutellarin had a promoting effect, in which the underlying mechanism referred to the upregulation of Fas-associated factor 1.
Unusual Painful Muscle Spasm in a Dialysis-Dependent Patient with Progressive Cervical Kyphosis
Authors: Tsunoda Daisuke , Iizuka Yoichi , Mieda Tokue , Iizuka Haku , Chikuda Hirotaka
Published: February 2019

We herein report a case of cervical myelopathy due to destructive spondyloarthropathy (DSA) with an unusual painful muscle spasm. A 51-year-old man presented to our hospital due to numbness in the hands and progressive gait disturbance. The patient underwent laminoplasty for cervical myelopathy. One and a half years after surgery, the patient developed progressive kyphosis of the cervical spine and increasing spastisity. He also started to suffer from intractable pain associated with a muscle spasm. A blood analysis revealed marked CK elevation. The patient underwent 2-stage circumferential decompression and fusion. After the revision procedure, his symptoms improved, and his serum CK level normalized. Physicians should be alert to the possibility of pain associated with an excessive muscle spasm in cervical myelopathy patients.
AsyncBTree: Revisiting Binary Tree Topology for Efficient FPGA-Based NoC Implementation
Authors: Vipin Kizheppatt
Published: February 2019

Binary tree topology generally fails to attract network on chip (NoC) implementations due to its low bisection bandwidth. Fat trees are proposed to alleviate this issue by using increasingly thicker links to connect switches towards the root node. This scheme is very efficient in interconnected networks such as computer networks, which use generic switches for interconnection. In an NoC context, especially for field programmable gate arrays (FPGAs), fat trees require more complex switches as we move higher in the hierarchy. This restricts the maximum clock frequency at which the network operates and offsets the higher bandwidth achieved through using fatter links. In this paper, we discuss the implementation of a binary tree-based NoC, which achieves better bandwidth by varying the clock frequency between the switches as we move higher in the hierarchy. This scheme enables using simpler switch architecture, thus supporting higher maximum frequency of operation. The effect on bandwidth and resource requirement of this architecture is compared with other FPGA-based NoCs for different network sizes and traffic patterns.
Optimizing Culture Conditions by Statistical Approach to Enhance Production of Pectinase from Bacillus sp. Y1
Authors: Guo Fenfen , Li Xuezhi , Zhao Jian , Li Guanxi , Gao Peike , Han Xiaolong
Published: February 2019

It was found that Bacillus sp. Y1 could secrete alkaline pectinase with suitable enzyme system for powerful and fast degumming of ramie fiber. In this study, the medium components and fermentation conditions were optimized by some statistical methods including mixture design, fractional factorial design, central composite design and response surface methodology, and single factor method for enhancing the alkaline pectinase production. The optimized conditions for pectinase production were that the culture was shaken at 34°C for 60 h in 50 mL of medium containing 10.5% (w/v) carbon source (consisting of 3.8% starch, 4.2% wheat bran, and 2.5% sucrose), 0.37% (NH4)2SO4, 0.3% MgSO4, and 0.1% Tween-80, with initial pH 8.2 and inoculation amount of 1.3 mL (with the OD600 of the seed medium about 5.77). Using the optimizing conditions, the activities of polygalacturonate lyase (PGL) and polygalacturonase (PG) in fermentation liquor were increased to 2.00-fold and 3.44-fold, respectively, and the fermentation time shortened 12 hours (from 72 h to 60 h), which showed good application potential in degumming of ramie.
Benefits of Vitamins in the Treatment of Parkinson’s Disease
Authors: Zhao Xiuzhen , Zhang Ming , Li Chunxiao , Jiang Xue , Su Yana , Zhang Ying
Published: February 2019

Parkinson’s disease (PD) is the second most common neurodegenerative disease in the elderly, which is clinically characterized by bradykinesia, resting tremor, abnormal posture balance, and hypermyotonia. Currently, the pathogenic mechanism of PD remains unclear. Numerous clinical studies as well as animal and cell experiments have found a certain relationship between the vitamin family and PD. The antioxidant properties of vitamins and their biological functions of regulating gene expression may be beneficial for the treatment of PD. Current clinical evidence indicates that proper supplementation of various vitamins can reduce the incidence of PD in the general population and improve the clinical symptoms of patients with PD; nevertheless, the safety of regular vitamin supplements still needs to be highlighted. Vitamin supplementation may be an effective adjuvant treatment for PD. In this review, we summarized the biological correlations between vitamins and PD as well as the underlying pathophysiological mechanisms. Additionally, we elaborated the therapeutic potentials of vitamins for PD.