bluuebunny/crossref_metadata_2025 huggingface dataset:
BOOK REVIEW | Baghdad Burning: Girl Blog from Iraq, Volume II
Authors: Jerome Alexandra Izabela
Published: October 2024

The uncensored sphere of the Internet has permitted the free exchange of ideas and rapid dissemination of information. At the core of Internet discourse is the blog, a new phenomena which allows politicos and novices alike to reach an audience in new, unmediated ways that conventional media often do not permit. In the Middle East, especially in those countries with institutionalized censorship and a rise in Islamization, the blog has provided citizens with an outlet through which the limitations of print media can be circumvented. One of the most famous of the blogs that has emerged in this climate of censorship, war, and religiosity is the Riverbend Blog, written by a 26 year-old woman in Baghdad. A former engineer who lost her job after the 2003 U.S.-led invasion of Iraq, Riverbend’s blog has given the world a glimpse into what journalists isolated in the Green Zone or embedded with coalition troops can never give us: the real Iraq. The second volume of Riverbend’s work follows her journey through the extraordinary and mundane of the Iraq war that began in her award-winning book, Baghdad Burning: Girl Blog from Iraq (2005). In this volume, which covers October 2004 through March 2006, her commentary and analysis deal with topics ranging from the 2004 U.S. presidential elections to the rise of Islamic fundamentalism in the streets of Iraq. In October 2004, the week preceding the elections, Riverbend writes what she calls “an open letter of sorts” (10) to the American people: “I guess what I’m trying to say is this: Americans, the name of your country which once stood for ‘freedom and justice’ is tarnished worldwide. Your latest president has proved that the great American image of democracy is just that—an image.” (13) After the re-election of George W. Bush, she expresses her disappointment in a series of political rhymes, a parody of the Hallmark phenomena. She is candid in her disgust and it is this bluntness in her posts that can make even the most liberal American bristle at the failure of the American people to reject the war through the Electoral College.
Darfur: Covering the “forgotten” story
Authors: Pintak Lawrence
Published: October 2024

There is no issue in Arab journalism today that is more controversial than how the region’s media cover Darfur. Not Iraq, where, according to a new report from the Arab Archives Institute, 52 Arab journalists have lost their lives since 2001; not Palestine, where journalists are caught between Israel and the Palestinians and between Fatah and Hamas; nor Lebanon, where reporters have been in the cross-hairs of rival factions and governments. Darfur is a hot-button issue in the newsroom not because of the physical danger but because the issue bores right to the heart of the mission of Arab journalism and the self-identity of those who practice it. That was vividly apparent at a one-day workshop on the crisis organized by the International Crisis Group and hosted by the Center for Electronic Journalism at The American University in Cairo in April this year and it was evident again, two weeks later, at the 2007 Arab Broadcast Forum, the annual gathering of Arab television executives. The central issue: “The Arabs see the victims are not Arabs, and we don’t care,” Khaled Ewais, Al Arabiya’s political producer, told the Cairo gathering, which brought together reporters and editors from across the Arab world. Fayez el Sheikh Saleik, Khartoum correspondent of Al-Hayat, concurred: “Sudan is a marginal country when it comes to the Arab region.”
BOOK REVIEW | Filming the Modern Middle East: Politics in the Cinemas of Hollywood and the Arab World
Authors: Abu-Remaileh Refqa
Published: October 2024

Lina Khatib’s Filming the Modern Middle East: Politics in the Cinemas of Hollywood and the Arab World builds on Jack Shaheen’s study of American film and media representations of Arabs in Reel Bad Arabs (2001) but broadens it to include the Arab film industry. While Shaheen critiqued portrayals of Arabs and Islam in Hollywood films, Khatib advances the critique by not only examining Hollywood representations of the Middle East, but also how Arabs represents themselves, their politics, and their crises in the cinema. Filming analyzes 70 American and Arab films covering the period from 1980 to 2005 that were selected because they “concerned themselves with the salient political issues of the time” (3). Such selection criteria are ambiguous, however, given that such salient issues have been treated in Arab films long before the 80s. The choice for periodisation leaves us to ponder the importance, if any, of 1980 as a marker in the development of film, especially in the Arab world, as opposed to earlier decades. The periodisation in Filming appears to be driven more by the selection of Hollywood films rather than their Arab counterparts. Conveniently, the majority of Hollywood films (23 of them) dealing with the Middle East fall into that period. However, the basis for the selection of Arab films dealing with these “salient political issues” is not elaborated on. Here it would have been useful if Khatib defined what it means for a film to be political, especially considering that so many Arab films deal with political issues in one way or another.
Radio Sawa: America’s new adventure in radio broadcasting
Authors: Hilmy Sam
Published: October 2024

In April 2002, the U.S. Government launched an audacious new Arabic language radio station aimed at the countries of the Middle East and North Africa. The round-the-clock broadcasts, oddly dubbed Radio Sawa, replaced at a single stroke the respected brand name of the Voice of America's Arabic Service, which had for over a half century, in war and peace, provided the region with comprehensive full service programming. A predominantly pop music service designed to appeal to youth, Sawa was established at the behest of American commercial media mogul Norman Pattiz who, until his resignation at the end of 2006, was a member of the Broadcasting Board of Governors (BBG), an independent U.S. federal agency. The BBG oversees all non-military U.S. Government-funded broadcast outlets. To run the new station, the BBG under Chairman Kenneth Tomlinson (a former chairman of the Corporation for Public Broadcasting, editor of Reader's Digest and director of the VOA who also recently abandoned his government position under a cloud of criticism for mismanagement) founded and funded the Middle East Broadcasting Networks (MBN), a non-profit corporation. MBN also operates Sawa's younger twin Arabic TV satellite channel Alhurra. Mr. Tomlinson has publicly described his friend and colleague Mr. Pattiz as "the father of Radio Sawa." After the station was launched, Mr. Pattiz described it in a public forum in ecstatic terms: "It sounded so different and it was so appealing—because it really sounds like a Western contemporary music station, a pop station."
Does the veiled look sell? Egyptian advertisers grapple with the hijab
Authors: Otterman Sharon
Published: October 2024

It is an axiom of advertising that, to be effective, an ad must feel and look natural—and yet at the same time represent a prettier, cleaner, better version of reality, a reality to which one can aspire. But if that’s true, who is the ‘Arab woman’, and who would she like to be? The representation of women in Arab television advertising is a mishmash of the diverse cultural currents buffeting the region today. On MBC4, for example, a popular Dubai-based satellite channel that shows mostly American sitcoms and programs, one frequently shown ad depicts an olive-skinned, unveiled Arab woman who tries a skin-lightening cream called Fair and Lovely and then finds a dream job in a department store. Another Arabic-language ad spotlights a beautiful veiled young mother dressed all in white, serving Knorr soup to her family in perfect domestic bliss. In a third, a gorgeous, tanned model in a skimpy sundress throws back a 7up while sunbathing on the concrete bank of a public fountain. Are all these ads targeting the same women, or different women? Do advertising companies have any strategy here, or are they just trying a variety of things to see what works?
The Changing Ambitions of Workers: Adapting Leadership for Today's Reality
Authors: Westover Jonathan
Published: October 2024

Recent research suggests workers' career ambitions are changing, with fewer aspiring to top executive roles like CEO. This article explores the implications of shifting ambitions for organizational leadership strategies. Research from McKinsey and others finds only 3 in 10 workers today want C-suite careers, down from previous generations where climbing the corporate ladder was standard. Societal factors like economic instability and greater valuation of work-life balance, as well as generational preferences for meaningful work and flexibility over prestige roles, help explain changing priorities. To optimize talent management amid these trends, the author recommends companies realign leadership strategies, talent practices, and culture. Distributed leadership models, non-linear career paths focused on skills development, and culture emphasizing purpose, learning, and well-being can better engage modern workers who prioritize autonomy, growth, and impact over rigid hierarchy. Industry examples demonstrate how redefining success and expanding career definitions can boost retention, diversity, and collaboration.
Segunda cita de la especie exótica Pheidole megacephala (Fabricius, 1793) (Hymenoptera, Formicidae) en la España peninsular
Authors: Espadaler Xavier , Pradera Carlos , Estrada Virginia
Published: October 2024

Pheidole megacephala (Fabricius, 1793) (Hymenoptera, Formicidae): a second record for continental Spain
Perancangan Sistem Keamanan Rumah Menggunakan Face Recognition (Pengenalan Wajah) Berbasis Raspberry Pi
Authors: Adi Nugraha Batubara  , Abdul Jabbar lubis  , Arnes Sembiring
Published: October 2024

Talking about technology, currently computer technology is developing very rapidly and is a field that has a very important role in several aspects of human life, including in the field of security. Currently, many home or room security systems have been developed with several identity verification methods using a computer system, using keys, cards, passwords, and so on. However, this method still has shortcomings such as humans' limitations in remembering number combinations which makes the door inaccessible. Therefore, techniques for reliable and accurate identification or verification can be designed using biometric technology that utilizes the special characteristics of the human individual.
Campanha de Sensibilização para a Lavagem das mãos
Authors: Coelho Paula
Published: October 2024

Inserido nas comemorações do Dia Internacional do Enfermeiro o grupo de "Higiene Hospitalar - Enfermagem na Prevenção e Controlo da Infecção Hospitalar", realizou de 7 a 11 de Maio de 2007 uma jornada de Sensibilização para a Lavagem das Mãos dirigida a todos dos profissionais da CSE, clientes, familiares e visitas. Esta iniciativa surgiu na sequência da análise dos resultados de um diagnóstico realizado à prática da lavagem das mãos na CSE - afinal, a que é tradicionalmente considerada o acto mais importante para a prevenção e controlo da infecção nosocomial.
Algoritmo para abordagem da via aérea difícil não previsível na grávida
Authors: Santos Ângela dos , Marinho Ana , Ramón Manuel , Lages Neusa , Correia Carlos
Published: October 2024

A falha de entubação na grávida é um problema grave a ter em conta em anestesia obstétrica porque contribui para a mortalidade materna e fetal. O ideal seria prever e planificar todas as entubações difíceis, o que não é possível porque os testes preditivos da via aérea nem sempre são fiáveis e por vezes o anestesiologista se depara com cenários de entubações difíceis não previsíveis. Como anestesiologistas, a antecipação é uma estratégia que faz parte da especialidade. Os autores propõem um algoritmo de abordagem da via aérea difícil não previsível , como plano alternativo a ter em mente para o manuseio dessas situações.
Graduação em Gestão de Unidades de Saúde: FOGUS I
Authors: Escoval Ana
Published: October 2024

Em 2010, foi celebrado um Protocolo de Colaboração entre a Escola Nacional de Saúde Pública da Universidade NOVA de Lisboa (ENSP/UNL) e a Clínica Sagrada Esperança (CSE), que visava dar formação em Gestão para Chefiar uma Formação em Secretariado Clínico, entre outras áreas científicas. Uniram-se vontades, competências e visões e foram desenhados vários tipos de formação que procuravam responder às necessidades formativas dos profissionais da CSE. Ao todo, foram envolvidos cerca de 180 profissionais.
Poster- Yellow Fever outbreak in Angola
Authors: Brock Nádia
Published: October 2024

Yellow fever is a vector-borne disease affecting humans and non-human primates in tropical areas of Africa and South Americ
CANCRO DO COLO DO ÚTERO EM ANGOLA: CASOS DE LUANDA E BENGO
Authors: Alfredo Edson Joaquim Mayer , Fernandes Ema C. Branco , Catumbela Emanuel , Campos Adão Paulo
Published: October 2024

Introdução: A magnitude do cancro cervical (CC) é desconhecida em Angola. Os dados reportados pelo IACC entre os anos 2007 e 2011, colocam o CC na segunda posição, com uma prevalência estimada em 16,5%. Este estudo teve como objectivo identificar a prevalência de lesões precursoras e de CC em mulheres em idade reprodutiva submetidas ao rastreio.Metodologia: Estudo observacional, descritivo, transversal, sobre rastreio de CC em Luanda (Maternidade Lucrécia Paím e Instituto Nacional de Controlo do Cancro e em Caxito, Bengo, Angola, entre os anos de 2012 e 2014. Variáveis: sociodemográficas, vida reprodutiva e sexual. Para a análise descritiva dos dados foi usado o Epi info, versão 3.2.5.Resultados: A prevalência de lesões foi de 31.7% (743/2341) em 2.341 processos analisados, distribuídas em 527 (23%) lesão de baixo grau, 204 (9%) lesão de alto grau, 12 (1%) cancro invasivo. A média de idade das mulheres foi de 40.8±11 anos, 1026 (44%) eram analfabetas ou com o ensino primário feito, 1337 (57%), com primeiro acto sexual entre os 14 e 17 anos, 51 (13%) referiram ter quatro ou mais parceiros prévios e 1058 (44%) com 5 a 10 gestações.Conclusão: A prevalência de lesões precursoras do CC é alta na população estudada. Medidas educativas massivas sobre a doença devem ser implementadas, associadas a programas de rastreio sistemáticos que garantam a detecção precoce de lesões precursoras, tratamento oportuno e seguimento das mulheres com a doença em estádio avançado.
DESAFIO CLÍNICO: SABE DO QUE SE TRATA?
Authors: Tomás Ndenga
Published: October 2024

Para o desafio desta edição, apresentamos o caso de um homem de 17 anos de idade, com queixas de evolução há vários meses com febrícula, tosse produtiva com expectoração hemoptoica, emagrecimento progressivo, dorsalgias e deformação da coluna (cifose).
PREVALÊNCIA DAS URGÊNCIAS E EMERGÊNCIAS CIRÚRGICAS NO HOSPITAL AMÉRICO BOAVIDA
Authors: Fernando Paulo , Amaral Filomena do , Silva Amílcar da
Published: October 2024

Introdução: A doença cirúrgica é tratada de forma inadequada globalmente, e as condições de emergência que exigem cirurgia contribuem substancialmente para o grande problema das doenças a nível mundial. Apesar dos inúmeros desafios, é notável que alguns países tem aumentado a capacidade de lidar com emergências cirúrgicas. Objectivo: Explorar as principaiscausas, número e características de emergências e urgências cirúrgicas, no bloco operatório de um hospital de nível terciário.
Metodologia: Foi realizado um estudo descritivo, observacional, retrospectivo, com amostra de conveniência de 371 registos operatórios extraídos do Bloco operatório do Hospital Américo Boavida seleccionados no 4º trimestre de 2017. Resultados: As cirurgias de urgência foram mais frequentes com 307 (82,7%) casos e as principais causas de urgência cirúrgica foram apendicite aguda e as fraturas expostas representando 24,8% e 22,6% respectivamente, com domínio do sexo masculino 277 (74,7%) casos e faixa etária jovem (19-35 anos, representando 40,7%,).
Conclusão: Elevada frequência de atendimento de cirurgias deurgência, sendo as causas de abdómen agudo como a apendicite aguda e peritonite secundária, bem como as traumáticas foram as mais prevalentes.
DESAFIO CLÍNICO: DO QUE SE TRATA?
Authors: Tomás Ndenga
Published: October 2024

Mulher de 35 anos de idade, acorre a consulta por emagrecimento. Sem outras queixas.Foi-lhe efectuado um check-up médico, com determinados exames laboratoriais e uma ecografia abdominal.Em seguida as imagens da ecografia abdominal e da tomografia abdominal que a paciente efectuou.Queremos saber qual o órgão afectado e quais os diagnósticos diferenciais?
PREVALÊNCIA DE INFECÇÃO NOSOCOMIAL NA UNIDADE DE CUIDADOS DIFERENCIADOS DA CLINICA SAGRADA ESPERANÇA
Authors: Silva Fortunato
Published: October 2024

Devido à relevância do problema da Infecção Nosocomial em Unidades de Cuidados Intensivos fez-se um estudo prospectivo para caracterizar a infecção nosocomial (IN), avaliar o impacto destas infecções na morbimortalidade de uma Unidade de Cuidados Intensivos Polivalente,  analisando as taxas de IN, calcular a mortalidade associada à IN e verificar os agentes etiológicos prevalentes nas IN da unidade. O estudo teve a duração de 6 meses (1 de Agosto de 2002 a 31 de Janeiro de 2003) durante o qual foram internados na UCI 187 doentes. Constituíram objecto do nosso estudo apenas 115 doentes (61,5 %). A Taxa de Infecção Nosocomial foi de 11,3%. A infecção nosocomial mais frequente foi a PNEUMONIA com 7 casos (53.8 %), seguida pela SÉPSIS (30.8%) e INFECÇÃO URINÁRIA com 15.4 %. A Taxa de incidência foi de 10.9‰ na Pneumonia  seguida pela Sépsis com 6.3‰ e  Infecção Urinária com 3.1‰. A média de estadia dos doentes com IN foi de 9.2 dias (dp = 5.6 ) aumentando em 5.25 dias o tempo médio de estadia dos doentes sem infecção nosocomial (p = 0.09). Os principais agentes isolados foram as bactérias gram negativas (Escherichia Coli e Proteus)  seguindo-se o   Staphilococus Aureus. Os doentes com Infecção Nosocomial apresentaram letalidade significativamente maior que os doentes sem infecção (p=0,19). O conhecimento da epidemiologia local das infecções nosocomiais é fundamental para que medidas de controle possam ser adoptadas com objectivo de reduzir a sua morbimortalidade
Ferramentas de Gestão (1): A Análise SWOT
Authors: Malheiro Jorge B.
Published: October 2024

Um bom ponto de partida para uma reflexão sobre o que desejaríamos que viesse a ser o futuro da nossa organização é a realização de uma «ANÁLISE SWOT». Não há registos precisos sobre a origem desse tipo de análise. Segundo HINDLE & LAW-RENCE (1994) a análise SWOT foi criada por dois professores da Harvard Business School: Kenneth Andrews e Roland Christensen. Por outro lado, TARAPANOFF (2001:209) indica que a ideia da análise SWOT já era utilizada há mais de três mil anos quando cita numa epígrafe um conselho de Sun Tzu: “Concentre-se nos pontos fortes, reconheça as fraquezas, agarre as oportunidades e proteja-se contra as ameaças ” Apesar de bastante divulgada e citada por autores, é difícil encontrar uma literatura que aborde directamente esse tema.
Bebé Colódio (Síndrome do bebé Arlequim) - A Propósito de um Caso Clínico
Authors: Sachissokele Francisco
Published: October 2024

A Ictiose de Arlequim ou Síndrome do Bebé Colódio é uma forma de Ictiose congénita relativamente rara. Tem-se muito pouca informação sobre a sua patogénese, evolução clínica, tratamento apropriado e prognóstico, porque a maior parte dos doentes morre poucas semanas após o nascimento, devido a complicações como sepsis e dificuldade respiratória. Apresentamos aqui o caso de uma menina com o Síndrome de Bebé de Arlequim. Ela apresentou-se com as manifestações cutâneas típicas de escamas em placas espessas, maciças, com fissuras, ectrópio e eclábio, deformidades dos pavilhões auriculares e extremidades distais dos membros. Após tratamento de suporte, a doente sobreviveu e está agora com quatro anos, mantendo actualmente apenas as lesões ictiosiformes. Este é o primeiro caso de Bebé Colódio em Angola com uma sobrevida longa.
ĐÔI ĐIỀU VỀ CHUYỂN THỂ ĐIỆN ẢNH
Authors: Phạm Mai
Published: October 2024

Chuyển thể (adaptation) thực ra đã tồn tại khá lâu. Hiện nay, ta thường được xem những bộ phim chuyển thể từ một truyện ngắn, một tiểu thuyết văn học hay một vở kịch. Có lẽ, bởi về lý thuyết, điện ảnh là nghệ thuật thừa hưởng nhiều đặc tính của 6 loại hình đứng trước nó, trong đó có thơ ca (văn học). Còn về thực tế, văn học và điện ảnh là hai loại hình nghệ thuật có những điểm tương đồng, chúng cùng kể một câu chuyện có nhân vật, không gian, thời gian với những hoàn cảnh, tình huống cụ thể. Có khác là, việc kể đó bằng những ngôn ngữ khác nhau, dẫn đến cách kể cũng khác nhau. Có thể coi văn học là nguồn tư liệu vô tận để điện ảnh khai thác, và cũng có nhiều nhà nghiên cứu văn học, điện ảnh bàn về vấn đề chuyển thể này.
Towards a paradigm shift in the fuctions of biologically active agents: herbicide-mediated hormesis
Authors: Kurchii B. A.
Published: October 2024

One of the most important points to which I pay particularly close attention in this paper is the action of biologically active chemicals (BACs) in the living things. Theoretically, the action of BACs manifests itself in four stages, which are determined by the doses from the smallest to the largest: absence of visible effects, stimulation of biochemical and growth processes, inhibition of growth processes and death of the living things. Today, scientific research is mainly focused on the second stage of BACs action. At the same time, using of herbicides (killers of unwanted plants) in agriculture give evidens that they stimulate the growth of beneficial plants. The paradigm of only the stimulating action of BACs should be replaced by the paradigm of events at all four stages of BACs action in living organisms. The article proposes the mechanisms of events at all four stages of BACs action. The main focus is on redox reactions. Mechanisms of transformation of inactive BACs into reactive agents in endogenous redox reactions are described. This article also aims to focus the discussion on the analysis of the diversity of mechanisms underlying the action of natural and synthetic chemical agents.
Design of potential Ilp markers for Hordeum vulgare and H. marinum Atg2, Atg7, Atg8 genes
Authors: Novozhylov D. O. , Hordynskyi S. O. , Rabokon A. M. , Demkovych A. Ye. , Pryvalikhin S. M. , Blume R. Y. , Buziashvili А. Yu. , Yemets A. I. , Pirko Ya. V.
Published: October 2024

Aim. Design of potential intron length polymorphism markers for ATG2, ATG7, ATG8 genes. Methods. Use of classical bioinformatics methods to search for loci encoding ATG2, ATG7, ATG8 in the Hordeum marinum genome based on homology with cultivated barley genes; analysis of exon-intron structure of genes, design of ILP markers. Results. The sequences of ATG2, ATG7, ATG8 genes in H. marinum were determined. Analyzed differences in the exon-intron structure of the ATG2, ATG7, ATG8 loci in cultivated (H. vulgare) and salt-resistant wild barley (H. marinum). 25 potential ILP markers were designed for introns that showed significant interspecies length polymorphism: 13 for ATG2, 6 for ATG7, 3 for ATG8A, 2 for ATG8B and 1 for ATG8D. Conclusions. Based on gene structure comparative analysis of the autophagosome formation proteins ATG2, ATG7, ATG8 of cultivated H. vulgare and salt-tolerasnt H. marinum, a number of introns are recommended for further use as a potential source of ILP markers for the study of interspecies genetic differentiation of cereals.
Setting of seeds during Agrobacterium-mediated winter wheat transformation by in planta method
Authors: Dubrovna O. V. , Slivka L. V.
Published: October 2024

Aim. To investigate the frequency of seeds setting and the formation of transgenic plants during Agrobacterium-mediated transformation of winter wheat by the in planta method using different methods of applying agrobacteria and the composition of inoculation media. Methods. Agrobacterium-mediated transformation by in planta method, molecular genetic analysis, methods of mathematical statistics. Results. A significant difference was found in the studied genotypes of winter wheat according to the indicator seed setting depending on the A. tumefaciens strain used and the inoculation medium. When comparing the effect of different strains of agrobacteria on this indicator, no significant differences between them were established, however, a tendency to increase the frequency of seed formation was observed when using the LBA4404 strain. When using the MS-22 inoculation medium, which additionally contained 12.5 mM MES, 4 mM NH4CI, 5.5 mM MgSO4, a certain increase in the number of formed seeds was also observed compared to the MS-21 medium supplemented with sodium thiosulfate, the frequency of seed setting increased in on average by 5 %. Conclusions. The genotypic dependence of the frequency of seed setting and the formation of transgenic plants with the use of different inoculation media and the method of the transformation procedure was established.
Androgenesis in vitro in anther culture of bread winter wheat
Authors: Zambriborshch I. S. , Shestopal O. L. , Chekalova M. S. , Afinogenov O. A. , Lytvynenko M. A. , Vasiliev O. A.
Published: October 2024

Aim. Study of the effectiveness of stages of in vitro androgenesis in anther culture for the creation of dihaploid lines of bread winter wheat. Methods. In vitro culture of isolated wheat anthers. For each genotype, the following parameters of androgenesis in vitro were determined: the percentage of caluses and the percentage of regeneration of green plants from the number of planted anthers; the percentage of acclimatized plants from regenerated green plantlets, as well as the percentage of fertile (doubled haploid) plants. Results. Androgenesis in vitro in the anther culture of 105 genotypes of soft winter wheat of different genetic origin was studied. Differences regarding the frequency of callusogenesis induction (from 0.17 to 21.25 percent of planted anthers) and the ability to regenerate plants (from 0 to 4.94 percent of planted anthers) were found. The dihaploid lines in anther culturefor almost 50 % of the studied genotypes were obtained. Spontaneous diploidization averaged 29.41 % of the obtained regenerants. Conclusions. Genotype-specific morphogenetic reactions of bread winter wheat microspores in the process of in vitro androgenesis were revealed. 122 dihaploid lines of bread winter wheat were obtained.
Characteristics of lectin receptors on erythrocyte membranes of patients with glioblastoma
Authors: Karpova I. S. , Shuba I. M. , Lylo V. V. , Glavatskyi O. Y.
Published: October 2024

Aim. This work is devoted to the search the abnormalities of the receptor-lectin reactions associated with glioblastoma for diagnostic purposes. Methods. Red blood cell (RBC) specimens were taken from 22 persons distributed into the two cohorts: donors without an oncological diagnosis; patients with glioblastoma (GB). Panel of 9 commercial lectins (LECTINOTEST, Lviv, Ukraine) was used: ConA, WGA, PHA-P; PHA-E; PHA-L, SNA, VAA, STA, and LABA. Also the original lectin from persimmon sepals (named PSL) was used. It was obtained by isoelectric focusing and ammonium sulfate fractionation. Intensity of lectin-receptor interactions was determined in reaction of hemagglutination. Results. Тwo lectins showed some directed divergence in patients from donors. For wheat germ agglutinin (WGA) сhanges in the intensity of lectin-receptor binding were downward. In the case of persimmon L (PSL) the alternative result was observed: the intensity of hemagglutination showed a trend to exceed the level of control. Conclusions. Some individual features in the reaction of erythrocytes with a set of lectins are observed in all examined persons. For two studied lectins, namely WGA and PSL, unidirectional deviations were observed in the reaction of erythrocytes of patients with GB compared to individuals without an oncological diagnosis.
Prospects of using the recultivator composition Trevitan™ for obtaining and growing a collection of plants in vitro
Authors: Kolisnyk Kh. M. , Hrytsak L. R. , Prokopiak M. Z. , Boiko D. A. , Drobyk N. M.
Published: October 2024

Aim. To evaluate the effect of the composite recultivant preparation "TrevitanTM" on the germination of seeds of Carlina cirsioides Klok., Carlina onopordifolia Bess. ex Szaf., Kulcz. et Pawl., Carlina acaulis L., Gentiana verna L. and Gentiana lutea L. with extended shelf life and on the growth parameters of plants of these species in vitro cultures. Methods. In vitro cultivation methods, biometric methods. Results. It is shown that the effectiveness of the recultivant composition "TrevitanTM" on seed germination and plant growth in vitro depends on their species. The use of this drug stimulated the germination of G. lutea seeds with a 5-year extension of its germination period. However, it did not significantly affect the germination and germination energy of freshly collected seeds and seeds with a long period of preservation of germination of species of the genus Carlina. In combination with auxin (3-indoleacetic acid), the composite recultivant "TrevitanTM" inhibited the growth and development of the root system in plants of the species G. verna, C. acaulis and C. cirsioides, but stimulated it in C. onopordifolia. The introduction of this drug into the MC/2 nutrient medium without the use of other growth regulators stimulated the growth of internodes in vitro in plants of the mountain species G. verna, and in another mountain species – C. acaulis – it accelerated the development of new leaves and their growth in length. Conclusions. The obtained results indicate the need for further research on the effect of the composite recultivant "TrevitanTM" with the involvement of a wider species diversity of plants.
Seed germination of Cannabis sativa L. under artificially simulated salt stress in vitro culture
Authors: Mishchenko S. V.
Published: October 2024

Aim. The response of hemp (Cannabis sativa L.) to artificially simulated salt stress in vitro culture was the aim of our research. Methods. The seeds of the Hlyana variety were cultivated under conditions of salt stress, which was artificially simulated by adding a certain salt to the Murashige & Skoog nutrient medium in accordance with the main types of soil salinity: chloride, sulfate, and carbonate salinity. Results. Hemp clearly responded to an increase in the concentration of salts in the nutrient medium, in particular, a decrease in seed germination was observed. The most suitable for cultivation is the nutrient medium with sulfates, and the least – with carbonates (at relatively low concentrations). Conclusions. Test systems (including the concentrations of the studied salts) were developed and tested for salt stress screening. The test systems were selective in their selection. Selective concentrations of salts in the environment are determined as follows: chlorides – 0.25 NaCl, 0.75 % MgCl2 · 6 H2O; sulfates – 0.5 MgSO4 · 7 H2O, 1.0 % Na2SO4; carbonates – 0.15 Na2CO3, 0.30 % NaHCO3. The following straight-line regression equations have been constructed, allowing the use of model data to predict seed germination in practical agriculture, depending on the amount of increase / decrease in salt concentration in the soil.
Didactic possibilities of scientific and educational LabXchange online platforms for genetics learning in the pedagogical ZVO
Authors: Torianyk V. M. , Mironets L. P.
Published: October 2024

Aim. Highlight the possibilities of the scientific and educational platform LabXchange for distance learning of genetics for students of higher education in the specialties 014 Secondary Education (Biology and Human Health) and 091 Biology. Methods. Content analysis, survey, generalization. Results. LabXchange, an online educational platform created by scientists, provides users with free access to high-quality scientific education through a variety of online tools. LabXchange's Comprehensive General Genetics Curriculum for Higher Education Students includes content types that transform standard science material into engaging interactive visualizations that promote better understanding of complex concepts, provide the opportunity to conduct genetic experiments in virtual laboratories with state-of-the-art equipment, help personalize the learning process, making it interesting and accessible opens up new opportunities for developing critical thinking skills, creativity and independence. Conclusions. The LabXchange online scientific and educational platform is an innovative resource for the study of genetics for students of higher education, as it performs important didactic functions. The experience of its use by students of a pedagogical university is useful for the formation of their professional competences.
Problems and effectiveness of breeding of winter wheat varieties with increased environmental stability
Authors: Bazaliy V. V. , Lavrynenko Yu. O. , Domaratskyi E. O. , Larchenko O. V. , Panfilova A. V.
Published: October 2024

Aim. The search of adaptive response peculiarities to adverse environmental factors is an important condition of development of varietal technologies and control of adaptive potential of winter wheat varieties. Methods. The studies were carried out in accordance with the field test methodology. The wheat varieties of different types of growing, different genetic and ecological origins were studied. Results. The alternative variety Clarisa and the new universal varieties of winter wheat Perlyna, Askaniyska, Askaniyska Bereginya were characterized by the highest yield at different times of spring vegetation recovery. Thus, on average over the years of research, they exceeded the standard variety of Kherson Bezosta by 0.51–1.27 t/ha in terms of yield, and under unfavorable conditions of the late recovery of spring vegetation, their advantage was at the level of 0.58–1.34 t/ha. Conclusions. For stable production of high-yielding winter soft wheat grain, use new varieties of the universal type Askaniyska, Askaniyska Bereginya, Perlyna and alternative types Clarissa, Solomia for different agrophones for late sowing periods according to different predecessors.
The influence of flowering periods on productivity characteristics in breeding samples of soft winter wheat created with the involvement of western european ecotypes
Authors: Vozhegova R. A. , Marchenko T. Yu. , Lavrynenko Yu. O. , Bazalii H. Н. , Bazaliy V. V. , Mishchenko S. V.
Published: October 2024

Aim. To determine the nature of the influence of flowering periods on productivity traits in soft winter wheat lines, which were created with the involvement of late-ripening samples of the Western European ecotype. Methods. Research methods are field, laboratory, selection-genetic, statistical. Results. The nature of the manifestation of the "date of flowering" trait in hybrids F1-F3 and selection numbers selected from hybrid populations with the participation of Western European ecotypes of soft winter wheat, the correlation dependence of the date of flowering, grain yield, ear length and the duration of the "flowering-maturity" period were established. Optimums for the manifestation of indicators that determined high yield and ear length were established. Conclusions. For each hybrid population created with the participation of parental components contrasting in duration of vegetation, it is necessary to develop a specific selection plan taking into account intra-population correlations of yield, flowering dates and the duration of the "flowering-maturity" period.
Sunflower broomrape (Orobanche cumana Wallr.) damage in different hybrids of Helianthus annuus
Authors: Khablak S. H. , Spychak V. M.
Published: October 2024

Aim. To study the racial composition of broomrape in sunflower crops in the conditions of the Forest-Steppe and Polissya of Ukraine. Methods. Sunflower hybrids were evaluated for resistance to broomrape in soil culture using a modified method and the roll method of seed germination. Results. The results of the study revealed a different reaction of sunflower hybrids to the parasite. Sunflower hybrids Arizona, Transol, Bosphorus, resistant to race F, were moderately affected by broomrape. On average, there were 5 to 6 nodules of the parasite per sunflower plant. Sunflower hybrids Estrada, Kupava, Kadiks, Laskala, resistant to race G, were affected to a weak extent by broomrape. On average, there were 2-3 nodules of the parasite per sunflower plant. No sunflower hybrids with complete immunity to broomrape were found. Based on the research, the reasons for the widespread spread of broomrape in the fields in the central, northern and western regions of the country were identified. Conclusions. The emergence of new very aggressive races of broomrape (E, F and G) indicates an important need to solve the problem of creating breeding material resistant to new races of this parasitic plant. The intensive accumulation of the parasite races E, F and G in sunflower crops is associated with the violation of crop rotations and saturation of fields with hybrids of this crop, which are mainly resistant to races 5 (E) and 6 (F) of the parasite.
Bioinformatic study of keratinases from the S01 family from Streptomyces microflavus strain Dsm 40593
Authors: Polishchuk L. V.
Published: October 2024

Aim. Determination and study of the primary structures and domain organization of keratinase precursors (serine proteases from the S01 family) of S. microflavus DSM 40593 using bioinformatic methods. Methods. In the research information presented in databases on the NCBI server was analyzed. Computerized sequence analysis was performed using the BLAST program on the NCBI server. Results. BLAST analysis of the genome sequence of the strain S. microflavus DSM 40593 was carried out using the sequence of the precursor AGK81825 as a query. Thanks to bioinformative analysis, seven genes encoding precursors of serine proteases from the S01 family were found in the genome of the strain S. microflavus DSM 40593. Conclusions. In the genomes of streptomycetes, there may be more than one gene encoding precursors of keratinases from the S01 family. It was established that the amino acid sequences of the enzymatic domains of keratinase precursors from the S01 family are characterized by higher similarity indices than the sequences of foldase domains.
Features of the isolation of yeast from the root microbiome of winter wheat (Triticum aestivum L.)
Authors: Sokolova N. , Yemets A. I.
Published: October 2024

Aim. Development of approaches for isolating yeast symbionts from the rhizosphere of various varieties of winter wheat for their further taxonomic identification, as well as studying the influence of yeast species on the growth and development of wheat plants. Methods. Isolation and identification of yeast from the root microbiome of selected wheat varieties was carried out using microbiological and microscopic research methods. Results. As a result of the investigation, conditions were selected, isolation and primary identification of yeast from the root microbiome of winter wheat varieties in different growing periods was carried out. The following yeast species were identified: Saccharomyces serevisiae, Hanseniaspora uvarum, Papiliotrema terrestris, Pichia fementans, Candida subhashii, Torulaspora delbruecki and Candida sp. Conclusions. The difference in the species composition of yeast-symbionts in different periods of development of winter wheat varieties Lisova Pisnya, Pereyaslivka, Bohdana, Smuglyanka, Podolyanka, Kolonia, Reform and Rebel was noted. The obtained data emphasize the importance of studying the rhizosphere microbiome, in particular the functional role of yeast, for understanding the mechanisms of interaction between the plant and the soil, as well as for the development of effective agronomic strategies in the cultivation of wheat and other crops.
Influence of izatison on growth and development of Kalanchoe daigremontiana (Raim.-Hamet & Perrier) A. Berger
Authors: Katsan V. A. , Potopalsky A. I. , Zadorozhnii B. O.
Published: October 2024

Aim. The investigation was performed to studying of the influence of Izatison on the adaptiv morphogenesis in plants. Kalanchoe Daigremontiana was chosen as the model. Methods. The plants, obtained from the plantlets of same age and origin, were grown as a soil culture in separate pots. The mature leaves from the middle layer of the plants were used for the investigation. The first versions of Izatison dilutions were used. Results. The statistically reliable increase of the initiated plantlets on the leaf (P<0.05) was detected in the version with the greatest dilution for 5 days of exposition of the Kalanchoe leaves in the water suspensions of Izatison. The statistically reliable of the accelerated emergence of second pair of the leaves (P<0.05; P<0.01) was shown in tenth day in the versions with the middle ant the greatest dilutions. It was detected earlier emergence of the third pair of the leaves for 11–17 days (P<0,05; P<0,001) in the versions with the greatest dilutions. Conclusions. It was shown the possibility of Izatison to stimulate the initiation of plantlets and accelerate their differentiation and the growth depending from its concentration.
Studying the composition and anti-radiation properties of sage (Salvia officinalis L.) in wheat seeds
Authors: Shamılov E. N. , Abdullayev A. S. , Shamilli V. E. , Azizov I. V.
Published: October 2024

Aim. The purpose of the research was to study the chemical composition, develop methods for obtaining an extract from the leaves of Salvia officinalis L. and study their anti-radiation properties of irradiated wheat seeds of the Guneshli variety. Methods. Using qualitative reactions and chromatography, the content of lipids, essential oils, diterpene acids, phenolic compounds, macro- and microelements in plants was determined. Wheat seeds were irradiated using a URI installation (K-25) at a dose rate of 13.4 rad/sec, at a dose of 200 Gy. The amount of chlorophyll pigments, carotenoids, and malondialdehyde was measured using a spectrophotometer. Chlorophyll fluorescence in leaves was determined using a MINI-PAM device. Results. According to the results of morphological and physiological-biochemical parameters of seedlings, 0.01 % and 0.001 % Salvia officinalis extract has a positive effect on growth and development, increases the maximum quantum yield of PS II and reduces the yield of lipid peroxidation product. Conclusions. It has been established that Salvia officinalis extract has a radioprotective effect and can be used as a radioprotector for some agricultural plants.
Prediction of protein-ligand binding sites modulating activity of MAST protein kinases
Authors: Karpov P. A. , Steshenko A. O. , Ozheredov S. P. , Blume Y. B.
Published: October 2024

Aim. Identification of the protein-ligand binding sites, that may be the target of compounds, affecting individual human protein kinases of the MAST family (MAST1, 2, 3, 4 and MASTL / GWL). Methods. Literature and database search. Comparison of protein and ligand structures. Protein structure modeling, structural superimposition, etc. Results. The structural alignment demonstrates significant similarity of catalytic domains in MAST1, 2, 3, 4 and MASTL (GWL). It justifies transferring of reference ligands from PDB structures to human MASTs, discovering potential sites of ligand binding. 13 sites of ligand-binding were specified based on refrence ligands, transferred from RCSB Protein Data Bank structures, and differences in sites amino acid composition of MAST family members were discovered. Сonclusions. Based on the differences in the amino acid composition of the studied pockets in MAST1, 2, 3, 4 and MASTL (GWL), the sites B, C, D, E, F, were selected for further study and virtual screening for new selective inhibitors of individual members of MAST protein kinase family.
Structural features of carrot α-tubulin predetermining the natural resistance to dinitroaniline herbicides
Authors: Melnyk O. G. , Ozheredov S. P. , Blume Y. B. , Karpov P. A.
Published: October 2024

Aim. To explain the natural resistance of Daucus carota L. to dinitroaniline herbicides. To clarify features of the carrot α-tubulin that may affect formation of the ligand-protein complex based on the structural and electrostatic analysis of the ligand-binding site. Methods. Reconstruction of the spatial structure of α-tubulin from D. carota and Toxoplasma gondii using profile (Swiss-Model) and de-novo (AlphaFold2) modeling. Molecular dynamics (MD) simulations of the built 3D-models in Gromacs. Analysis of the molecular electrostatics with PDB2PQR/APBS tools. Visualization and analysis of molecular structures in PyMOL. Results. It has been shown that along with the typical positive charge of the dinitroaniline-binding pocket, all isotypes of carrot α-tubulin demonstrate negatively charged regions that may cause conflicts with the nitro-groups of the ligands. Also, the MD-stable negatively charged "bridge" between Cys316 and the aryl-fragment of Phe255 was observed in all α-tubulin isotypes. In our opinion, it not only competes with the cyclic fragment of dinitroanilines, but overall prevent the opening of the site pocket in carrot α-tubulin. Conclusions. It was clarified that natural resistance of D. carota to dinitroaniline herbicides may be associated with steric and electrostatic conflicts observed in in all α-tubulin isotypes. In our opinion, it prevents interaction with dinitroaniline compounds at the stage of primary site recognition on the early stages of protein-ligand complex formation.
Introducción a la “viscehermenéutica”: lectura pornográfica en "Los detectives salvajes" de Roberto Bolaño
Authors: Grzesiak Zofia
Published: October 2024

Este artículo presenta un fragmento del paradigma de las estrategias y prácticas de lectura de los personajes de Roberto Bolaño, con el objetivo de incorporar al autor en un diálogo teórico-literario. El análisis del estilo de recepción de Juan García Madero, personaje de Los detectives salvajes (1998), permite observar la conexión del intelecto y el cuerpo, la hermenéutica (Gadamer) y las “vísceras” esenciales en esta obra. La masturbación del protagonista, causada por la lectura de un poema, provoca la discusión del modo “pornográfico” de leer (Ullén) y de diversos placeres textuales (Barthes) en oposición a la interpretación convencional. Asimismo, constituye el punto de partida para el análisis cognitivo del fenómeno de la recepción, inmersión y la comunicación literaria (Gerrig), en la que los protagonistas de Bolaño pretenden tomar un rol activo. Su estilo de recepción es híbrido, combina la capacidad analítica con las vísceras del lector, une el cuerpo, el intelecto, las emociones, y la intuición, asimismo que considera un libro como una instrucción que debe ser aplicada en el arte y en la vida. Proponemos describir este modo de leer, en homenaje al viscerrealismo, como “viscehermenéutica”.
Walker Carlos. (2022). Contra Bolaño, Santiago de Chile: Lecturas Ediciones
Authors: Velázquez Soto Armando
Published: October 2024

Reseña del libro "Contra Bolaño"
Making memories
Authors: Longo Francesco
Published: October 2024

The neurotransmitter dopamine helps form long-term memories by increasing the production of proteins through a unique signaling pathway.