bluuebunny/crossref_metadata_2025 huggingface dataset:
The posttraumatic cognitive appraisal inventory (PTCAI): development and validation
Authors: Zhang Wenyue , Yu Wenjing , Wei Baojian , Dong Qianni , Zhang Aihua
Published: December 2023

Objective

This study aims to develop and validate the Posttraumatic Cognitive Appraisal Inventory (PTCAI) for accidental trauma survivors.

Method

Based on interviews and expert feedback, the initial item pool was generated for the Negative Cognitive Appraisal Inventory of Loss and Feeling Threatened, and the Positive Cognitive Appraisal Inventory of Positively Face, Self-Sense, and Relationships. Then, we recruited two groups of accidental trauma survivors to examine the reliability and validity of the PTCAI. Item analysis and exploratory factor analysis (EFA) were conducted on Sample 1. Confirmatory factor analysis (CFA), Pearson correlation analysis, and internal consistency reliability analysis were applied to Sample 2. After 2 weeks, 20 survivors completed the PTCAI again to test temporal stability.

Results

Following item analysis, the PTCAI was reduced to 27 items. The results of the EFA demonstrated that the five-factor, 27-item solution of the PTCAI was appropriate, which accounted for 63.931% of the total variation. The CFA indicated that the five-factor second-order model offered an excellent fit to the data. Loss and Feeling threatened were equally important in the study participants’ negative cognitive appraisal of accidental traumas. Self-sence was the most important positive cognitive appraisal of accidental traumas by the study participants. Positively Face and Relationships were somewhat behind. Additionally, the PTCAI demonstrated high concurrent validity and reliability (test–retest and internal consistency).

Conclusion

The PTCAI appears to be a reliable and valid instrument for assessing cognitive appraisals of accidental trauma survivors.
Risk attitude and belief updating: theory and experiment
Authors: Huang Evelyn Y. H. , Leung Benson Tsz Kin
Published: December 2023

Despite the importance of risk attitude in decision-making, its role in belief updating has been overlooked. Using economic theory, we analyzed a dual-self equilibrium where an individual first updates her belief about an uncertain state and then takes an action to maximize her payoff. We showed that stronger risk aversion drives more conservative actions and thus decreases the instrumental value of information relative to the importance of belief-based utility. As a result, the relationship between risk attitude and belief updating depends on the nature of the belief-based utility. With self-relevant information, stronger risk aversion leads to more belief change, whereas with self-irrelevant information, stronger risk aversion leads to less belief change. Our experimental results concur with the theoretical predictions with two settings where subjects update their belief about their IQ and a randomly drawn number, respectively. We discuss implications on persuasion, advertisements, and political campaigns.
Hierarchical structures emerge from the cultural transmission: an iterated learning experiment using a non-linguistic task
Authors: Nakata Seiya , Takezawa Masanori
Published: December 2023

Human language is characterized by complex structural features, such as the hierarchical combination of words to form sentences. Although other animals use communication systems, empirical evidence of hierarchical structures is rare. Computational studies of language evolution have suggested that cultural transmission plays a key role in the emergence of structural features in human languages, including hierarchy. While the previous study demonstrated the emergence of hierarchical structures in non-linguistic systems, we argue that their laboratory study may have overestimated the role of cultural transmission because of a lack of appropriate controls and analyses. To directly test the effect of cultural transmission, we conducted an experiment with no cultural transmission as a control (individual condition) in addition to replicating the previous transmission experiment (transmission condition). Our study has added a quantitative analysis of the hierarchical depth. We found that sequences became more structured as the number of generations increased; however, those produced under the transmission condition were more structured than those under the individual condition. These findings suggest that cultural transmission plays an important role in the emergence of hierarchical structures, which cannot be explained by increased learnability alone. The emergence of complex structural properties in human culture, such as language, technology, and music, may have resulted from information transmission processes between different individuals. In conclusion, this study provides evidence of the crucial role of cultural transmission in the emergence of hierarchical structures in non-linguistic communication systems. Our results contribute to the ongoing debate on the origins of human language and the emergence of complex cultural artifacts. The results of this study have implications for the study of cultural evolution and the role of transmission in shaping the emergence of structural features across diverse domains.
Appraising systematic reviews: a comprehensive guide to ensuring validity and reliability
Authors: Shaheen Nour , Shaheen Ahmed , Ramadan Alaa , Hefnawy Mahmoud Tarek , Ramadan Abdelraouf , Ibrahim Ismail A. , Hassanein Maged Elsayed , Ashour Mohamed E. , Flouty Oliver
Published: December 2023

Systematic reviews play a crucial role in evidence-based practices as they consolidate research findings to inform decision-making. However, it is essential to assess the quality of systematic reviews to prevent biased or inaccurate conclusions. This paper underscores the importance of adhering to recognized guidelines, such as the PRISMA statement and Cochrane Handbook. These recommendations advocate for systematic approaches and emphasize the documentation of critical components, including the search strategy and study selection. A thorough evaluation of methodologies, research quality, and overall evidence strength is essential during the appraisal process. Identifying potential sources of bias and review limitations, such as selective reporting or trial heterogeneity, is facilitated by tools like the Cochrane Risk of Bias and the AMSTAR 2 checklist. The assessment of included studies emphasizes formulating clear research questions and employing appropriate search strategies to construct robust reviews. Relevance and bias reduction are ensured through meticulous selection of inclusion and exclusion criteria. Accurate data synthesis, including appropriate data extraction and analysis, is necessary for drawing reliable conclusions. Meta-analysis, a statistical method for aggregating trial findings, improves the precision of treatment impact estimates. Systematic reviews should consider crucial factors such as addressing biases, disclosing conflicts of interest, and acknowledging review and methodological limitations. This paper aims to enhance the reliability of systematic reviews, ultimately improving decision-making in healthcare, public policy, and other domains. It provides academics, practitioners, and policymakers with a comprehensive understanding of the evaluation process, empowering them to make well-informed decisions based on robust data.
Decomposing user-defined tasks in a reinforcement learning setup using TextWorld
Authors: Petsanis Thanos , Keroglou Christoforos , Ch. Kapoutsis Athanasios , Kosmatopoulos Elias B. , Sirakoulis Georgios Ch.
Published: December 2023

The current paper proposes a hierarchical reinforcement learning (HRL) method to decompose a complex task into simpler sub-tasks and leverage those to improve the training of an autonomous agent in a simulated environment. For practical reasons (i.e., illustrating purposes, easy implementation, user-friendly interface, and useful functionalities), we employ two Python frameworks called TextWorld and MiniGrid. MiniGrid functions as a 2D simulated representation of the real environment, while TextWorld functions as a high-level abstraction of this simulated environment. Training on this abstraction disentangles manipulation from navigation actions and allows us to design a dense reward function instead of a sparse reward function for the lower-level environment, which, as we show, improves the performance of training. Formal methods are utilized throughout the paper to establish that our algorithm is not prevented from deriving solutions.
Supplementary posterior fusion in patients operated on employing TLIF may decrease the instrumentation failure rate
Authors: Bokov Andrey , Kalinina Svetlana , Khaltyrov Mingiyan , Pavlova Svetlana , Bulkin Anatoliy
Published: December 2023

Background

It is supposed that additional posterior fusion may provide additional stability of the pedicle screw; however, the clinical impact of additional posterior fusion in patients treated with TLIF remains uncertain. The objective of this study is to assess the clinical efficacy of circumferential fusion in patients treated with TLIF.

Materials and methods

This is a single-center retrospective evaluation of consecutive 179 patients with degenerative lumbar stenosis and instability of spinal segments. Patients with axial pain and neurogenic claudication or radiculopathy associated with spinal stenosis were enrolled during the period from 2012 to 2018. Transforaminal lumbar interbody fusion (TLIF) with a single cage was used to treat patients. In 118 cases a supplementary posterior fusion was made. The duration of follow-up accounted for 24 months, logistic regression analysis was used to assess factors that influence the complication rate.

Results

The rate of pedicle screw loosening was growing with radiodensity getting decreased and was more frequent in patients with two level fusion. An increase in pedicle screw loosening rate correlated with anterior nonunion Tan 2 and 3 grade while both posterior complete and incomplete fusion resulted in a decline in the complication rate. Lumbosacral fusion, bilateral facet joints` resection and laminectomy turned out to be insignificant factors. The overall goodness of fit of the estimated general multivariate model was χ2 = 87.2230; P < 0.0001. To confirm clinical relevance of those findings, a univariate logistic regression was performed to assess the association between clinically significant pedicle screw instability and posterior fusion in patients operated on employing TLIF. The results of logistic regression analysis demonstrate that additional posterior fusion may decrease the rate of instrumentation failure that requires revision surgery in patients treated with TLIF [B0 = 1.314321; B1 = −3.218279; p = 0.0023; OR = 24.98507; 95% CI (3.209265; 194.5162), the overall goodness of fit of the estimated regression was χ2 = 22.29538, p = <0.0001].

Conclusion

Circumferential fusion in patients operated on employing TLIF is associated with a decline in the rate of pedicle screw loosening detected by CT imaging and clinically significant instrumentation failure.
Case Report: Recurrent colonic metastasis from lung cancer—diagnostic pitfalls and therapeutic challenge of a peculiar case
Authors: Tramontano Salvatore , Sarno Gerardo , Prisco Vera , Tedesco Anna Mirea , Gargiulo Antonio , Bracale Umberto
Published: December 2023

Lung cancer (LC) mortality exceeds 20%, and detecting metastases from LC is becoming a challenging step in understanding the real prognostic role of specific localization. We report a case of a patient with lung metastasis to the colon with local recurrence at the anastomosis after radical resection for metastasis. In both cases, the diagnosis was on oncological follow-up, and surgery was offered in consideration of reasonable life expectancy, good control of LC, and high risk of intestinal occlusion. A 67-year-old male, with a history of LC 18 months ago, was referred to our surgical unit after a positron emission tomography CT total body, where an area of intense glucose metabolism (SUV max: 35.6) at the hepatic colic flexure was reported. A colonoscopy revealed an ulcerated, bleeding large neoplasm distally to hepatic flexure, almost causing resulting total occlusion. Histologic examination revealed a tumor with complete wall thickness infiltration, which appears extensively ulcerated, from poorly differentiated squamous carcinoma (G3), not keratinizing, with growth in large solid nests, often centered by central necrosis. Two of the 30 isolated lymph nodes were metastatic. The omental flap and resection margins were free from infiltration. The malignant cells exhibited strong positive immunoreactivity only for p40. The features supported metastatic squamous carcinoma of lung origin rather than primary colorectal adenocarcinoma. After 8 months from surgery, intense Fluorodeoxyglucose (FDG) uptake of tissue was confirmed in the transverse colon. Colonoscopy evidenced an ulcerated substenotic area that involved ileocolic anastomosis on both sides. Reoperation consisted of radical resection of ileocolic anastomosis with local lymphadenectomy and ileotransverse anastomosis. The second histologic examination also revealed poorly differentiated squamous carcinoma (G3), not keratinizing, with positive immunoreactivity only for p40, suggesting the origin of LC. This case report confirmed that the possibility of colonic secondary disease should be part of the differential diagnosis in asymptomatic patients and those with a history of LC diagnosis. In addition, relapse of colonic metastasis is infrequent but should be considered during follow-up of LC. More studies on colonic metastasis of LC are required to better understand the clinical features and outcomes.
Can Comprehensive Medical Reform Improve the Efficiency of Medical Resource Allocation? Evidence From China
Authors: Sun Xiaoyang , Xu Liang , Adnan K. M. Mehedi , Luo Yusen
Published: December 2023

Objectives: To evaluate the impact of comprehensive medical reform on the efficiency of medical resource allocation in China.

Methods: This study employs the Slacks-Based Measure- Directional Distance Function (SBM-DDF) to estimate the efficiency of medical resource allocation (MRAE) in China, using panel data from 30 provinces during 2009–2021. Moreover, a multi-period Difference in differences (DID) model is developed to explore the effect of the comprehensive medical reform pilot (CMRP) strategy on efficiency of medical resource allocation in China.

Results: The results show that the average value of China’s medical resources allocation efficiency is 0.861 during the sample period. Coastal area has a higher MRAE than that in the inland area. The DID results show that the comprehensive medical reform pilot strategy has a good, long-lasting impact on the efficiency of medical resource allocation. And the results remain valid after a series of robustness analysis. Additionally, the comprehensive medical reform policy has heterogeneous impact on efficiency of medical resource allocation. The promotion effect is only statistically significant in the eastern and central regions, the groups of higher MRAE and larger population size.

Conclusion: China’s comprehensive medical reform policy can effectively promote the improvement of regional efficiency of medical resource allocation.
Delay in the Air or Detour on the Ground?—A Case Study in Guangzhou Baiyun International Airport
Authors: Du Zhuoming , Zhang Junfeng , Ma Zhao , Xu Jiaxin
Published: December 2023

Collaboration between terminal airspace and airport surface operation shows an increasing significance for the best efficiency of both parts of the air traffic management domain. Runways play a critical role in connecting the two parts for departure and arrival aircraft. Suppose the gate and the entry fix of an aircraft are predetermined according to the flight plan, and they are on the opposite side of the airport terminal. The aircraft will either spend more time (i.e., delay in the air) landing on a runway close to its gate or take a longer distance (i.e., detour on the ground) taxiing to its gate if a runway close to its entry fix is assigned. This paper proposes a runway assignment model considering terminal airspace operation and airport surface movement simultaneously to discover how runway assignments can affect integrated operations. Four different runway assignment schemes are applied in this model. Subsequently, a metaheuristic method is proposed to solve the model. Furthermore, the historical taxiing and flight time data are analyzed to demonstrate the potential benefits of runway reassignment. Finally, the results show that the free assignment of the runway stands out among the four schemes, not only in the performance of terminal airspace operation (lower flight time) but also in airport surface movement (lower pushback delay, taxi time).
Oxidative Stress and Redox-Dependent Pathways in Cholangiocarcinoma
Authors: Caligiuri Alessandra , Becatti Matteo , Porro Nunzia , Borghi Serena , Marra Fabio , Pastore Mirella , Taddei Niccolò , Fiorillo Claudia , Gentilini Alessandra
Published: December 2023

Cholangiocarcinoma (CCA) is a primary liver tumor that accounts for 2% of all cancer-related deaths worldwide yearly. It can arise from cholangiocytes of biliary tracts, peribiliary glands, and possibly from progenitor cells or even hepatocytes. CCA is characterized by high chemoresistance, aggressiveness, and poor prognosis. Potentially curative surgical therapy is restricted to a small number of patients with early-stage disease (up to 35%). Accumulating evidence indicates that CCA is an oxidative stress-driven carcinoma resulting from chronic inflammation. Oxidative stress, due to enhanced reactive oxygen species (ROS) production and/or decreased antioxidants, has been recently suggested as a key factor in cholangiocyte oncogenesis through gene expression alterations and molecular damage. However, due to different experimental models and conditions, contradictory results regarding oxidative stress in cholangiocarcinoma have been reported. The role of ROS and antioxidants in cancer is controversial due to their context-dependent ability to stimulate tumorigenesis and support cancer cell proliferation or promote cell death. On these bases, the present narrative review is focused on illustrating the role of oxidative stress in cholangiocarcinoma and the main ROS-driven intracellular pathways. Heterogeneous data about antioxidant effects on cancer development are also discussed.
Pharmacological Property and Cytotoxic Effect Showing Antiproliferative Potency in Human Melanoma Cell Lines (A375) of Combretum racemosum P. Beauv. Leaf and Root Extracts Used in Benin
Authors: Dah-Nouvlessounon Durand , Chokki Michaelle , Hoteyi Ismaël M. S. , Fassinou Fidèle , Ranga Floricuta , Fetea Florinela , Diaconeasa Zorita , Vodnar Dan , Furdui Bianca , Baba-Moussa Farid , Dinica Rodica Mihaela , Suharoschi Ramona , Baba-Moussa Lamine
Published: December 2023

Combretum racemosum, a plant from the Combretaceae family, is traditionally used in Benin for various health problems. However, scientific research on Beninese samples of this plant is limited. The aim of this study was to identify and assess the bioactive compounds in the plant’s leaves and roots. Initial screening involved analyzing powders derived from these parts for total polyphenols, flavonoids, and both condensed and hydrolyzable tannins. The polyphenolic compounds were analyzed using HPLC-DAD-ESI-MS. To evaluate the plant’s antimicrobial properties, the agar diffusion method was employed, while FRAP and DPPH assays were used to determine its antioxidant capacity. For anti-inflammatory activity, the study utilized tests for in vitro protein denaturation inhibition and in vivo acute edema induced by carrageenan. Additionally, an antiproliferative assay was conducted using the human melanoma cell line A375. The analysis revealed the presence of significant polyphenolic compounds in both the leaf and root extracts of C. racemosum. Notably, compounds like Pedunculagin, Vescalagin, Casuarictin, and Digalloyl-glucoside were abundant in the leaves, with Vescalagin being especially predominant in the roots. The study also found that the dichloromethane extracts from the leaves and roots exhibited bactericidal effects on a substantial percentage of meat-isolated strains. Moreover, the antioxidant activities of these extracts were confirmed through FRAP and DPPH methods. Interestingly, the dichloromethane root extract showed strong activity in inhibiting thermal albumin denaturation, while the water–ethanol leaf extract demonstrated significant edema inhibition. Finally, the study observed that C. racemosum extracts reduced cell viability in a dose-dependent manner, with leaf extracts showing more pronounced antiproliferative effects than root extracts. These findings highlight the potential of C. racemosum leaves and roots as sources of compounds with diverse and significant biological activities. In conclusion, C. racemosum’s leaves and roots exhibit promising biological activities, highlighting their potential medicinal value.
Evaluating Antioxidant Performance, Biosafety, and Antimicrobial Efficacy of Houttuynia cordata Extract and Microwave-Assisted Synthesis of Biogenic Silver Nano-Antibiotics
Authors: Moorthy Kavya , Chang Kai-Chih , Huang Hsiao-Chi , Wu Wen-Jui , Chiang Cheng-Kang
Published: December 2023

From the traditional Chinese medicine point of view, although Houttuynia cordata extract (HCE) possesses an incredible amount of phytonutrients and exhibits antioxidant activities, excessive doses of HCE can cause danger to organisms and lead to death. In this study, we first examine HCE’s overall phenolic and flavonoid content, antioxidant efficacy, and antibacterial activity. Results show that HCE is suitable as a bio-reducing agent for the microwave-assisted synthesis of silver nanoparticles (HCE-AgNPs) with enhanced antioxidant and antimicrobial performance. Under an optimized microwave condition (i.e., 100 °C for 10 min), the HCE-stabilized AgNPs were confirmed with a UV-visible peak at 430 nm and 19.7 ± 4.2 nm in size. Physicochemical properties of HCE-AgNPs were extensively characterized by zeta-potential, FT-IR, XRD, and XPS measurements. Compared to the HC extract counterpart, HCE-AgNPs display superior antioxidant activity, higher DPPH scavenging efficiency, and enhanced broad-spectrum bactericidal activity to inhibit the growth of all tested bacterial strains at doses of 2 μg/mL. Biosafety evaluation indicated that HCE-AgNPs are noncytotoxic on human red blood cells. These data show that the microwave synthesis of AgNPs exhibits a great antioxidant ability, superior antibacterial activity, and a trivial hemolytic effect, providing another bactericidal therapy strategy to address the increasing healthcare-associated infections.
Updates on Aβ Processing by Hsp90, BRICHOS, and Newly Reported Distinctive Chaperones
Authors: Iqbal Mohammed , Lewis Shea-Lorane , Padhye Shivani , Jinwal Umesh Kumar
Published: December 2023

Alzheimer’s disease (AD) is an extremely devastating neurodegenerative disease, and there is no cure for it. AD is specified as the misfolding and aggregation of amyloid-β protein (Aβ) and abnormalities in hyperphosphorylated tau protein. Current approaches to treat Alzheimer’s disease have had some success in slowing down the disease’s progression. However, attempts to find a cure have been largely unsuccessful, most likely due to the complexity associated with AD pathogenesis. Hence, a shift in focus to better understand the molecular mechanism of Aβ processing and to consider alternative options such as chaperone proteins seems promising. Chaperone proteins act as molecular caretakers to facilitate cellular homeostasis under standard conditions. Chaperone proteins like heat shock proteins (Hsps) serve a pivotal role in correctly folding amyloid peptides, inhibiting mitochondrial dysfunction, and peptide aggregation. For instance, Hsp90 plays a significant role in maintaining cellular homeostasis through its protein folding mechanisms. In this review, we analyze the most recent studies from 2020 to 2023 and provide updates on Aβ regulation by Hsp90, BRICHOS domain chaperone, and distinctive newly reported chaperones.
Procoagulant Status and Fibrinolytic Activity in COVID-19 Patients during Illness and Convalescence
Authors: Beznoshchenco Olga S. , Romanov Andrey Yu. , Dolgushina Nataliya V. , Gorodnova Elena A. , Ivanets Tatiana Yu. , Yarotskaya Ekaterina L. , Pyregov Aleksey V. , Grachev Sergej V. , Sukhikh Gennady T.
Published: December 2023

SARS-CoV-2 (Severe Acute Respiratory Syndrome-related CoronaVirus 2) activates the immune system, causing thrombin dysregulation and tissue damage and reduces endothelium anticoagulant function, leading to excessive thrombin formation. Hypercoagulability, which causes multiple organ failure in critically ill COVID-19 (COronaVIrus Disease 2019) patients, can be detected by viscoelastic tests like thromboelastography and rotational thromboelastometry (ROTEM). We aimed to assess the coagulation system status and fibrinolytic activity using ROTEM thromboelastometry in patients with COVID-19 and convalescents. The observational prospective study included 141 patients with COVID-19: Group 1—patients with mild (n = 39), Group 2—patients with moderate (n = 65), and Group 3—patients with severe (n = 37) COVID-19. The coagulation status was assessed twice—during the disease and in convalescence. The male gender, age > 56 years, overweight, and obesity were risk factors for developing severe COVID-19. During the disease in patients with moderate and severe COVID-19, the hemostatic system was characterized by a procoagulant status, which persists during the period of convalescence. Fibrinolysis shutdown was detected in both moderate and severe patients with COVID-19. The procoagulant status of the coagulation system and the shutdown of fibrinolysis are typical for patients with moderate to severe COVID-19. In convalescents, activation of coagulation remains, which indicates the need to monitor the hemostatic system after Illness.
Experimental Investigation of a Hybrid Device Combining a Wave Energy Converter and a Floating Breakwater in a Wave Flume Equipped with a Controllable Actuator
Authors: Martinelli Luca , Capovilla Giulio , Volpato Matteo , Ruol Piero , Favaretto Chiara , Loukogeorgaki Eva , Andriollo Mauro
Published: December 2023

This paper presents a hydrodynamic investigation carried out on the “Wave Attenuator” device, which is a new type of floating breakwater anchored with piles and equipped with a linear Power Take Off (PTO) mechanism, which is typical for wave energy converters. The device is tested in the wave flume, under regular waves, in slightly non-linear conditions. The PTO mechanism, that restrains one of the two degrees of freedom, is simulated through an actuator and a programmable logic controller with preassigned strategy. The paper presents the system identification procedure followed in the laboratory, supported by a numerical investigation essential to set up a credible control strategy aiming at maximizing the wave energy harvesting. The maximum power conversion efficiency under the optimal PTO control strategy is found: it is of order 50–70% when the incident wave frequency is lower than the resonance one, and only of order 20% for higher frequencies. This type of experimental investigation is essential to evaluate the actual efficiency limitations imposed by device geometry.
Consensus-Based Coordinated Control of Flexible Interconnected DC Microgrid Clusters
Authors: Qin Dandan , Wang Chao , Liu Yuliang , Wu Rundong , Hou Dengshan , Huang Weijian , Chen Liangliang
Published: December 2023

This paper proposes a consensus–coordinated control strategy to improve the stability and reliability of interconnected direct current (DC) microgrid cluster systems based on isolated bidirectional DC–DC converters. Compared with the traditional communication-based control strategy, the proposed method does not require an additional communication system. The proposed method is only based on local measurement information, making the whole cluster form an organic unit. No matter where the power disturbance occurs in the continuous-flow microgrid, all the balance units in the system can be linked to provide emergency power support for the disturbed microgrid, improve the dynamic characteristics of the DC voltage of the disturbed microgrid, and reduce the steady-state deviation of the DC voltage of the disturbed grid. The disturbance power is reasonably borne by the balance unit of the whole system according to its equivalent power distribution coefficient ratio. Finally, the effectiveness of the proposed control strategy is verified through the PSCAD/EMTDC simulation.
Incidence of Non-Melanoma Skin Cancers in Salento (Southern Italy): A 15-Year Retrospective Analysis from the Cancer Registry of Lecce
Authors: Sordi Emiliano , Piscitelli Prisco , Albanese Carla , Melcarne Anna , Tardio Anna , Quarta Fabrizio , Greco Enrico , Miani Alessandro , Falco Andrea , De Matteis Elisabetta , Congedo Maurizio , Civino Adele
Published: December 2023

Background and Objectives: Non-melanoma skin cancers (NMSCs) include basal cell carcinoma (BCC) and squamous-cell carcinoma (SCC), as well as a wide range of rare skin tumors. NMSCs is the most frequently diagnosed type of tumor among Caucasians. We aimed at estimating the incidence and mortality of NMSCs in the Salento area (Lecce province, Southern Italy), whose population is assumed to experience heavy and frequent sun exposure due to climatic/environmental factors, both for working and leisure activities. Materials and Methods: We computed the incidence of NMSCs in the Province of Lecce by examining the comprehensive real-world data collected by the local cancer registry, which covers all the 830,000 inhabitants, over a period of fifteen years (from 2003 to 2017), with a focus on the latest 5 years (2013–2017) for the analysis of the different histologic morphologies of these tumors. The incidence of NMSCs has been described in terms of absolute frequencies, crude rates and age-adjusted direct standardized rates (DSR). Joinpoint analysis was used to examine temporal trends in the incidence of NMSCs and estimate annual percent changes (APCs). Results: During the period of 2003–2017, the incidence of NMSCs reached a direct standardized rate (DSR) of 162.62 per 100,000 in men (mortality 1.57 per 100,000) and 89.36 per 100,000 in women (mortality 0.52 per 100,000), respectively. The incidence significantly increased among both men and women across the entire period. Basal cell carcinoma (BCC), with its different morphologies, represented about 67.6% of the NMSCs in men (n = 2139 out of a total of 3161 tumors observed between 2013 and 2017) and about 75.8% of the NMSCs in women (n = 1718 out of a total of 2264 tumors from 2013 to 2017), thus accounting for the vast majority of NMSCs. The results are consistent with the literature data carried out both at national and international level. Conclusions: Proper monitoring of this phenomenon through timely reporting and recording of all new NMSC cases is necessary to develop new preventive strategies.
Health and Bioactive Compounds of Fermented Foods and By-Products
Authors: Carvalho Anna Paula Azevedo de , Conte-Junior Carlos Adam
Published: December 2023

Some microorganisms can increase the levels of vitamins, antioxidant compounds, peptides, exopolysaccharides, organic acids, and other bioactive molecules in foods after fermentation [...]
Adaptation of a Commercial Qualitative BAX® Real-Time PCR Assay to Quantify Campylobacter spp. in Whole Bird Carcass Rinses
Authors: Bodie Aaron R. , Dittoe Dana K. , Applegate Savannah F. , Stephens Tyler P. , Ricke Steven C.
Published: December 2023

Poultry is the primary reservoir of Campylobacter, a leading cause of gastroenteritis in the United States. Currently, the selective plating methodology using selective agars, Campy Cefex and Modified Charcoal Cefoperazone Deoxycholate agar, is preferentially used for the quantification of Campylobacter spp. among poultry products. Due to the specific nature of Campylobacter, this methodology is not sensitive, which can lead to skewed detection and quantification results. Therefore, Campylobacter detection and quantification methods are urgently needed. The objective was to develop a shortened enrichment-based quantification method for Campylobacter (CampyQuant™) in post-chill poultry rinsates using the BAX® System Real-Time PCR assay for Campylobacter. The specificity and sensitivity for the detection of C. jejuni, C. coli, and C. lari in pure culture were determined. The BAX® System Real-Time PCR assay consistently detected and identified each species 100% of the time with an enumeration range of 4.00 to 9.00 Log10 CFU/mL. Enrichment time parameters for low-level concentrations (0.00, 1.00, and 2.00 Log10 CFU/mL) of Campylobacter using the BAX® System Real-Time PCR assay were elucidated. It was determined that an enrichment time of 20 h was needed to detect at least 1.00 Log10 CFU/mL of Campylobacter spp. Using the BAX® System Real-Time PCR assay for Campylobacter. As a result, time of detection, detection limits, and enrichment parameters were used to develop the CampyQuant™ linear standard curve using the detected samples from the BAX® System Real-Time PCR assay to quantify the levels in post-chill poultry rinsates. A linear fit equation was generated for each Campylobacter species using the cycle threshold from the BAX® System Real-Time PCR assay to estimate a pre-enrichment of 1.00 to 4.00 Log10 CFU/mL of rinsates detected. The statistical analyses of each equation yielded an R2 of 0.93, 0.76, and 0.94 with a Log10 RMSE of 0.64, 1.09, and 0.81 from C. jejuni, C. coli, and C. lari, respectively. The study suggests that the BAX® System Real-Time PCR assay for Campylobacter is a more rapid, accurate, and efficient alternative method for Campylobacter enumeration.
Analysis of the Diversity Presented by Vitis vinifera L. in the Volcanic Island of La Gomera (Canary Archipelago, Spain) Using Simple Sequence Repeats (SSRs) as Molecular Markers
Authors: Fort Francesca , Lin-Yang Qiying , Valls Carla , Sancho-Galán Pau , Canals Joan Miquel , Zamora Fernando
Published: December 2023

La Gomera Island is one of the areas of our planet where the phylloxera plague never arrived. To measure the genetic diversity of the vine after more than 500 years (inter- and intravarietal variability) of adaptation to this new environment, a prospection was carried out. For this purpose, 120 samples were collected and genotyped with 20 SSRs. A total of 52 unique profiles were found corresponding to 4 new varieties (Coello blanca, Barrerita negra, Malvasia periquin gomerae, Verdello gomerae), 9 individuals identical to the most widespread profile, and 39 individuals that presented variations (1 corresponding to a mutation of a new variety (Verdello gomerae de Monacal) and 38 corresponding to variations of known varieties, some of which included cases of triallelism or quadriallelism). The population of local vines in La Gomera Island is considered to be the most unique in the Canary Islands to date. It is hypothesised that the grapevine varieties Malvasia periquin gomerae and Verdello gomerae are possibly the most unique and that the Barrerita negra variety may have resulted from an interspecific crossbreeding. The Coello blanco variety (admixed) seems to have a strong Central European influence. Finally, we propose that the prime name for the Albillo forastero variety, which was arbitrarily imposed by the scientific community, be changed to the more widespread and better-known name in La Gomera Island and the Canary Archipelago, which is Forastera gomerae.
Accumulation of Inflammatory Mediators in the Normal Pericardial Fluid
Authors: El-Diasty Mohammad M. , Rodríguez Javier , Pérez Luis , Eiras Sonia , Fernández Angel L.
Published: December 2023

There is paucity of studies that focus on the composition of pericardial fluid under resting conditions. The purpose of this study is to determine the levels of inflammatory mediators in pericardial fluid and their correlation with plasma levels in patients undergoing elective cardiac surgery. We conducted a prospective cohort study on candidates for elective aortic valve replacement surgery. Pericardial fluid and peripheral venous blood samples were collected after opening the pericardium. Levels of interleukin 1α (IL-1α); interleukin 1β (IL-1β); interleukin 2 (IL-2) interleukin 4 (IL-4); interleukin 6 (IL-6); interleukin 8 (IL8); interleukin 10 (IL10); tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), vascular endothelial growth factor (VEGF), monocyte chemotactic protein-1 (MCP-1) epidermal growth factor (EGF), soluble E-selectin, L-selectin, P-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) were determined in both pericardial fluid and serum samples. A total of 45 patients with a mean age of 74 years were included of which 66% were males. Serum levels of all study mediators were within normal limits. Serum and pericardial levels of IL-1 α, IL-1 β, IL-2, IL-4, and IL-10 were similar. Levels of VEGF, EGF, VCAM-2, ICAM 1, E-selectin, P-selectin, and L-selectin were significantly lower in pericardial fluid than in serum. However, levels of IL-6, IL-8, TNF-α, IFN-γ, MCP-1, and MCP-1 were significantly higher in the pericardial fluid than in serum. Under normal conditions, the pattern of distribution of different inflammatory mediators in the pericardial fluid does not reflect serum levels. This may either reflect the condition of the underlying myocardium and epicardial fat or the activity of the mesothelial and mononuclear cells present in pericardial fluid.
Diel and Seasonal Changes in the Abundance and Diversity of the Infralittoral Fish Community in the Eastern Central Adriatic
Authors: Soldo Alen , Paliska Dejan
Published: December 2023

The fish species undergo diel and seasonal changes in coastal (littoral) ocean zones. Many factors affect these seasonal and diel patterns, thus it is difficult to determine which are the most important. Concerning the Adriatic Sea, studies on the temporal changes of fish communities are rare. Therefore, the primary aim of this study was to evaluate, in terms of abundance, diversity and species composition, the diel and seasonal changes in a one-year cycle of the fish community living in the infralittoral zone of the Eastern Central Adriatic and the main drivers affecting them. This study revealed the presence of a very rich and diverse community in the coastal zone of the Eastern Central Adriatic with a total of 63 identified fish species. The results showed that the differences in fish assemblage were more strongly influenced by seasonal variations rather than diurnal variations. The primary environmental factor that drives fish to undertake seasonal variations is water temperature. Considering that many species are important commercial species, especially for small scale fisheries, and taking into account future climate-driven changes that would affect the seasonality of the target species, it is essential that future management will be tailored accordingly and in due time.
Study on Mooring Design of 15 MW Floating Wind Turbines in South China Sea
Authors: Chen Mingsheng , Jiang Jiale , Zhang Wei , Li Chun Bao , Zhou Hao , Jiang Yichen , Sun Xinghan
Published: December 2023

Wind turbines and floating platform upsizing are major trends in the current offshore wind development. However, harsh environmental conditions increase the risk of anchor dragging and mooring failure when deploying large offshore floating wind turbines. Therefore, it is necessary to design a mooring system for the specific deployment site. This study aims to perform the mooring system design of a floating offshore wind turbine (FOWT) operated in the South China Sea, which is a combination of the IEA 15 MW wind turbine and UMaine VolturnUS-S floating platform. Hydrodynamic coefficients were calculated based on the potential flow theory, considering the environmental loads in the South China Sea. Additionally, the hydrodynamic coefficients were imported into AQWA to calculate the time-domain mooring tension. The mooring design parameters, such as mooring line length, nominal sizes, and anchor point, were determined using the criterion of anchor uplift, maximum breaking strength, and fatigue life, respectively. The design criterion required that the anchor uplift is not more than the allowable value, the long-term breaking limit of mooring with a 100-year return period should be less than the maximum breaking limit, and the fatigue damage accumulation in 50 years should be safe. The mooring design procedure provides a reference for mooring system design and safe operation of large floating wind turbines in the South China Sea.
An Extension of the Akash Distribution: Properties, Inference and Application
Authors: Gómez Yolanda M. , Firinguetti-Limone Luis , Gallardo Diego I. , Gómez Héctor W.
Published: December 2023

In this article we introduce an extension of the Akash distribution. We use the slash methodology to make the kurtosis of the Akash distribution more flexible. We study the general probability density function of this new model, some properties, moments, skewness and kurtosis coefficients. Statistical inference is performed using the methods of moments and maximum likelihood via the EM algorithm. A simulation study is carried out to observe the behavior of the maximum likelihood estimator. An application to a real data set with high kurtosis is considered, where it is shown that the new distribution fits better than other extensions of the Akash distribution.
Reversible Data Hiding in Encrypted Images Based on Two-Round Image Interpolation
Authors: Zhang Qing , Chen Kaimeng
Published: December 2023

The data embedding of vacating room after encryption reversible data hiding in encrypted images (VRAE RDHEI) is performed on an encrypted image without redundancy and spatial correlation. Data extraction and image recovery rely on a range of unique mechanisms that utilize spatial correlation in the decrypted domain. Of these mechanisms, pixel prediction is among the most frequently used, directly affecting the capacity and fidelity. In this paper, we propose a novel method that uses a two-round interpolation mechanism to enhance pixel prediction precision while preserving a large number of carrier pixels. In the proposed method, the content owner uses a stream cipher to encrypt the image as a carrier. The data hider flips specific LSBs of the encrypted image for data embedding. On the receiver side, the process of data extraction and image recovery is divided into two stages. In each stage, based on the varying distributions of the original or recovered pixels with the carrier pixels, the corresponding pixel interpolation schemes are used to accurately predict the pixels for data extraction and image recovery. The results demonstrate that the proposed method can efficiently improve the capacity and fidelity with full reversibility compared to existing VRAE RDHEI methods.
A Stackelberg Game-Based Model of Distribution Network-Distributed Energy Storage Systems Considering Demand Response
Authors: Li Zezhong , Peng Xiangang , Xu Yilin , Zhong Fucheng , Ouyang Sheng , Xuan Kaiguo
Published: December 2023

In the context of national efforts to promote country-wide distributed photovoltaics (DPVs), the installation of distributed energy storage systems (DESSs) can solve the current problems of DPV consumption, peak shaving, and valley filling, as well as operation optimization faced by medium-voltage distribution networks (DN). In this paper, firstly, a price elasticity matrix based on the peak and valley tariff mechanism is introduced to establish a master–slave game framework for DN-DESSs under the DPV multi-point access environment. Secondly, the main model optimizes the pricing strategy of peak and valley tariffs with the objective of the lowest annual operating cost of the DN, and the slave model establishes a two-layer optimization model of DESSs with the objective of the maximum investment return of the DESSs and the lowest daily operating costs and call the CPLEX solver and particle swarm optimization algorithm for solving. Finally, the IEEE33 node system is used as a prototype for simulation verification. The results show that the proposed model can not only effectively reduce the operating cost of the distribution network but also play a role in improving the energy storage revenue and DPV consumption capacity, which has a certain degree of rationality and practicality.
Open Quantum Dynamics: Memory Effects and Superactivation of Backflow of Information
Authors: Benatti Fabio , Nichele Giovanni
Published: December 2023

We investigate the divisibility properties of the tensor products Λt(1)⊗Λt(2) of open quantum dynamics Λt(1,2) with time-dependent generators. These dynamical maps emerge from a compound open system S1+S2 that interacts with its own environment in such a way that memory effects remain when the environment is traced away. This study is motivated by the following intriguing effect: one can have Backflow of Information (BFI) from the environment to S1+S2 without the same phenomenon occurring for either S1 and S2. We shall refer to this effect as the Superactivation of BFI (SBFI).
Investigating Novel IspE Inhibitors of the MEP Pathway in Mycobacterium
Authors: Choi Seoung-Ryoung , Narayanasamy Prabagaran
Published: December 2023

In a recent effort to mitigate harm from human pathogens, many biosynthetic pathways have been extensively evaluated for their ability to inhibit pathogen growth and to determine drug targets. One of the important products/targets of such pathways is isopentenyl diphosphate. Isopentenyl diphosphate is the universal precursor of isoprenoids, which are essential for the normal functioning of microorganisms. In general, two biosynthetic pathways lead to the formation of isopentenyl diphosphate: (1) the mevalonate pathway in animals; and (2) the non-mevalonate or methylerythritol phosphate (MEP) in many bacteria, and some protozoa and plants. Because the MEP pathway is not found in mammalian cells, it is considered an attractive target for the development of antimicrobials against a variety of human pathogens, including Mycobacterium tuberculosis (M.tb). In the MEP pathway, 4-diphosphocytidyl-2-c-methyl-d-erythritol kinase (IspE) phosphorylates 4-diphosphocytidyl-2-C-methyl-D-erythritol (CDPME) to form 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P). A virtual high-throughput screening against 15 million compounds was carried out by docking IspE protein. We identified an active heterotricyclic compound which showed enzymatic activity; namely, IC50 of 6 µg/mL against M.tb IspE and a MIC of 12 µg/mL against M.tb (H37Rv). Hence, we designed and synthesized similar new heterotricyclic compounds and tested them against mycobacterium, observing a MIC of 5 µg/mL against M. avium. This study will provide the critical insight necessary for developing novel antimicrobials that target the MEP pathways in pathogens.
Epidemiology of Leptospira sp. Infection: Current Status, Insights and Future Prospects
Authors: de Azevedo Sérgio Santos
Published: December 2023

In recent decades, the scientific community has been faced with an increased risk of emerging or re-emerging zoonotic diseases, such as leptospirosis, mainly originating from anthropic actions [...]