bluuebunny/crossref_metadata_2025 huggingface dataset:
Hyperprolactinemia in Adults with Prader-Willi Syndrome
Authors: Sjöström Anna , Pellikaan Karlijn , Sjöström Henrik , Goldstone Anthony P. , Grugni Graziano , Crinò Antonino , De Graaff Laura C. G. , Höybye Charlotte
Published: August 2021

Prader-Willi syndrome (PWS) is a rare neurodevelopmental genetic disorder typically characterized by body composition abnormalities, hyperphagia, behavioural challenges, cognitive dysfunction, and hypogonadism. Psychotic illness is common, particularly in patients with maternal uniparental disomy (mUPD), and antipsychotic medications can result in hyperprolactinemia. Information about hyperprolactinemia and its potential clinical consequences in PWS is sparse. Here, we present data from an international, observational study of 45 adults with PWS and hyperprolactinemia. Estimated prevalence of hyperprolactinemia in a subset of centres with available data was 22%, with 66% of those related to medication and 55% due to antipsychotics. Thirty-three patients were men, 12 women. Median age was 29 years, median BMI 29.8 kg/m2, 13 had mUPD. Median prolactin was 680 mIU/L (range 329–5702). Prolactin levels were higher in women and patients with mUPD, with only 3 patients having severe hyperprolactinemia. Thyroid function tests were normal, 24 were treated with growth hormone, 29 with sex steroids, and 20 with antipsychotic medications. One patient had kidney insufficiency, and one a microprolactinoma. In conclusion, severe hyperprolactinemia was rare, and the most common aetiology of hyperprolactinemia was treatment with antipsychotic medications. Although significant clinical consequences could not be determined, potential negative long-term effects of moderate or severe hyperprolactinemia cannot be excluded. Our results suggest including measurements of prolactin in the follow-up of adults with PWS, especially in those on treatment with antipsychotics.
Assessment of Dysphonia in Children with Pompe Disease Using Auditory-Perceptual and Acoustic/Physiologic Methods
Authors: Crisp Kelly D. , Neel Amy T. , Amarasekara Sathya , Marcus Jill , Nichting Gretchen , Korlimarla Aditi , Kishnani Priya S. , Jones Harrison N.
Published: August 2021

Bulbar and respiratory weakness occur commonly in children with Pompe disease and frequently lead to dysarthria. However, changes in vocal quality associated with this motor speech disorder are poorly described. The goal of this study was to characterize the vocal function of children with Pompe disease using auditory-perceptual and physiologic/acoustic methods. High-quality voice recordings were collected from 21 children with Pompe disease. The Grade, Roughness, Breathiness, Asthenia, and Strain (GRBAS) scale was used to assess voice quality and ratings were compared to physiologic/acoustic measurements collected during sustained phonation tasks, reading of a standard passage, and repetition of a short phrase at maximal volume. Based on ratings of grade, dysphonia was present in 90% of participants and was most commonly rated as mild or moderate in severity. Duration of sustained phonation tasks was reduced and shimmer was increased in comparison to published reference values for children without dysphonia. Specific measures of loudness were found to have statistically significant relationships with perceptual ratings of grade, breathiness, asthenia, and strain. Our data suggest that dysphonia is common in children with Pompe disease and primarily reflects impairments in respiratory and laryngeal function; however, the primary cause of dysphonia remains unclear. Future studies should seek to quantify the relative contribution of deficits in individual speech subsystems on voice quality and motor speech performance more broadly.
Age-Related Changes in Left Ventricular Vortex Formation and Flow Energetics
Authors: Chan Jeffrey Shi Kai , Lau Dawnie Ho Hei , Fan Yiting , Lee Alex Pui-Wai
Published: August 2021

Analysis of the cardiac vortex has been used for a deeper understanding of the pathophysiology in heart diseases. However, physiological changes of the cardiac vortex with normal aging are incompletely defined. Vector flow mapping (VFM) is a novel echocardiographic technique based on Doppler and speckle tracking for analysis of the cardiac vortex. Transthoracic echocardiography and VFM analysis were performed in 100 healthy adults (33 men; age = 18–67 years). The intracardiac flow was assessed throughout the cardiac cycle. The size (cross-sectional area) and circulation (equivalent to the integral of normal component of vorticity) of the largest vortices in systole (S-vortex), early diastole (E-vortex), and late diastole (A-vortex) were measured. Peak energy loss (EL) was calculated from information of the velocity vector of intracardiac flow in systole and diastole. With normal aging, the circulation (p = 0.049) of the E-vortex decreased, while that of the A-vortex increased (both p < 0.001). E-vortex circulation correlated directly to e’ (p = 0.003), A-vortex circulation correlated directly to A and a’ (both p < 0.001), and S-vortex circulation correlated directly to s’ (p = 0.032). Despite changes in vortex patterns, energy loss was not significantly different in older individuals. Normal aging is associated with altered intracardiac vortex patterns throughout the cardiac cycle, with the late-diastolic A-vortex becoming physiologically more dominant. Maintained energy efficiency accompanies changes in vortex patterns in aging hearts.
Comprehensive Use of Routine Clinical Parameters to Identify Patients at Risk of New-Onset Atrial Fibrillation in Acute Myocardial Infarction
Authors: Raczkowska-Golanko Monika , Raczak Grzegorz , Gruchała Marcin , Daniłowicz-Szymanowicz Ludmiła
Published: August 2021

(1) Background: New-onset atrial fibrillation (NOAF) is a significant complication of acute myocardial infarction (AMI). Our study aimed to investigate whether routinely checked clinical parameters aid in NOAF identification in modernly treated AMI patients. (2) Patients and methods: Patients admitted consecutively within 2017 and 2018 to the University Clinical Centre in Gdańsk (Poland) with AMI diagnosis (necrosis evidence in a clinical setting consistent with acute myocardial ischemia) were enrolled. Medical history and clinical parameters were checked during NOAF prediction. (3) Results: NOAF was diagnosed in 106 (11%) of 954 patients and was significantly associated with in-hospital mortality (OR 4.54, 95% CI 2.50–8.33, p < 0.001). Age, B-type natriuretic peptide (BNP), C-reactive protein (CRP), high-sensitivity troponin I, total cholesterol, low-density lipoprotein cholesterol, potassium, hemoglobin, leucocytes, neutrophil/lymphocyte ratio, left atrium size, and left ventricular ejection fraction (LVEF) were associated with NOAF in the univariate logistic analysis, whereas age ≥ 66 yo, BNP ≥ 340 pg/mL, CRP ≥ 7.7 mg/L, and LVEF ≤ 44% were associated with NOAF in the multivariate analysis. (4) Conclusions: NOAF is a multifactorial, significant complication of AMI, leading to a worse prognosis. Simple, routinely checked clinical parameters could be helpful indices of this arrhythmia in current invasively treated patients with AMI.
Mercury Accumulation and Elimination in Different Tissues of Zebrafish (Danio rerio) Exposed to a Mercury-Supplemented Diet
Authors: Vieira Hugo C. , Rodrigues Andreia C. M. , Soares Amadeu M. V. M. , Abreu Sizenando , Morgado Fernando
Published: August 2021

In this study, we evaluated the bioaccumulation of mercury in zebrafish (Danio rerio) exposed to mercury-contaminated food for 21 days and the depuration of mercury for a subsequent post-exposure period of 28 days. Four tissues (muscle, liver, gills, and skin) were analyzed for mercury content. Overall, data indicated that Hg accumulation in the liver is faster than in other tissues. Furthermore, the liver is the tissue with the highest accumulation rate per day (0.021 µg Hg g−1 day−1), followed by muscle, skin, and gills. Conversely, the Hg depuration rates in different tissues showed the following order: gills > skin > muscle > liver. The bioaccumulation factor values of liver and muscle increased linearly during the uptake period. The ratios between mercury concentration in liver and muscle during the experiment also increased during the uptake period and remained higher than 1 during the elimination period, suggesting that Danio rerio needed more than 4 weeks of depuration. Finally, the distribution of Hg in the water column during the accumulation period is Hg particulate > Hg dissolved, and during the depuration period it is the opposite, mercury particulate < mercury dissolved. In conclusion, this study contributes to a better understanding of the differences in Hg dynamics during the accumulation and depuration stages in a model fish, also emphasizing the alterations on Hg available in the water column.
Oral Candidal Colonization in Patients with Different Prosthetic Appliances
Authors: Kinkela Devcic Maja , Simonic-Kocijan Suncana , Prpic Jelena , Paskovic Igor , Cabov Tomislav , Kovac Zoran , Glazar Irena
Published: August 2021

Oral infections caused by Candida species are becoming more common, which may be related to an increase in the number of immunologically compromised patients as well as favorable conditions in the oral cavity that often include removable prosthetic appliances. The purpose of this study was to determine the presence of a particular Candida species in patients with PMMA and Cr-Co prosthetic appliances, as well as the salivary flow rate, and oral signs and symptoms. This investigation included a total of 120 subjects with different kinds of removable dentures. A sample of concentrated oral rinse was collected from all subjects in order to detect Candida colonization and identify the Candida species, a quantum of salivation was measured, and subjects were examined clinically. Candida spp. was predominant among the subjects who were denture wearers (p < 0.0001). In all subjects, the most frequently detected species was C.albicans. A statistically significant difference was found between the prevalence of C.albicans (p < 0.001) and C.krusei (p < 0.001) in denture wearers. Subjects with PMMA-based removable prosthetic appliances mostly demonstrated a significant decrease in salivation (p < 0.001), an increase in burning sensations (p < 0.001), and dry mouth (p < 0.001) compared to the subjects who wore partial dentures with Co–Cr metallic frameworks. Red oral lesions were more frequently found among the subjects with partial dentures with Co–Cr metallic frameworks (p < 0.001). Regardless of the material used for the denture, patients must be regularly checked by their dentists in order to prevent the development of oral lesions.
Efficacy of Selective Laser Trabeculoplasty after iStent Implantation in Primary Open-Angle Glaucoma
Authors: Siedlecki Adam R , Hicks Patrice M , Haaland Benjamin , DeAngelis Margaret M , Sieminski Sandra F
Published: August 2021

iStent implantation is thought to augment the trabecular outflow channel in the anterior segment of the eye. We hypothesized that iStent with subsequent selective laser trabeculoplasty (SLT) would better control the intraocular pressure (IOP) compared to standalone SLT in patients with primary open-angle glaucoma (POAG). We, therefore, determined if the presence of an iStent combined with SLT was statistically associated with IOP lowering compared to standalone SLT. Through retrospective electronic medical record review, records of 824 eyes from 440 patients who received primary SLT without a history of iStent were considered. Additionally, 42 eyes from 28 patients who received SLT after combined phacoemulsification and iStent implantation that failed to control intraocular pressure (IOP) and/or the progression of the disease were retrospectively reviewed. IOP and number of medications, which were tracked in each patient for up to 12 months post laser, were also examined. Successful outcome was defined as a statistically significant reduction in IOP or number of medications at 6 months. As defined in univariate analysis (p ≤ 0.01), multivariate analysis included iStent, age, sex, race, and initial IOP as variables. IOP reduction was statistically associated with patients pre-SLT IOP (p < 0.001) but not with patients with iStent (p = 0.222). Medication reduction was statistically associated with the pre-SLT number of medications (p < 0.001) and iStent (p < 0.001). In eyes that received SLT, iStent was not statistically associated with a greater reduction in IOP compared to controls, but was associated with a higher reduction in the overall number of medications used 6 months after receiving SLT. The work presented should guide clinicians to consider SLT as an effective therapy after iStent implantation, in terms of glaucoma medication reduction in iStent patients, but clinicians should know that the presence of an iStent does not necessarily make subsequent SLT more effective at lowering IOP.
Pain Improvement in Parkinson’s Disease Patients Treated with Safinamide: Results from the SAFINONMOTOR Study
Authors: Santos García Diego , Yáñez Baña Rosa , Labandeira Guerra Carmen , Cimas Hernando Maria Icíar , Cabo López Iria , Paz González Jose Manuel , Alonso Losada Maria Gema , Gonzalez Palmás Maria José , Cores Bartolomé Carlos , Martínez Miró Cristina
Published: August 2021

Background and objective: Pain is a frequent and disabling symptom in Parkinson’s disease (PD) patients. Our aim was to analyze the effectiveness of safinamide on pain in PD patients from the SAFINONMOTOR (an open-label study of the effectiveness of SAFInamide on NON-MOTOR symptoms in Parkinson´s disease patients) study. Material and Methods: SAFINONMOTOR is a prospective open-label single-arm study conducted in five centers from Spain. In this analysis, a secondary objective of the study, the score in the KPPS (King´s Parkinson´s Disease Pain Scale) at V1 (baseline) and V4 (6 months ± 1 month) were compared. Wilcoxon´s rank sum test was performed to test the changes from V1 to V4. Results: Forty-four (88%) out of 50 PD patients (age 68.5 ± 9.12 years; 58% women; 6.4 ± 5.1 years from diagnosis) completed the study. The KPPS total score was reduced by 43.6% (from 40.04 ± 36.18 in V1 to 22.60 ± 21.42 in V4; p < 0.0001). By domains, improvement was observed in musculoskeletal (−35.9%; p = 0.009), fluctuation-related (−51.7%; p = 0.020), nocturnal (−46.1%; p = 0.001), discoloration and/or edema/swelling (−50.4%; p = 0.009) and radicular pain (−40.1%; p = 0.048). A total of 21 adverse events in 11 patients (22%) were reported, five being severe, but not related to safinamide. Conclusion: Safinamide is well tolerated and improves pain in PD patients at 6 months. Future studies are necessary to analyze the possible beneficial effect of safinamide on pain in PD patients.
Kaleka Agroforest in Central Kalimantan (Indonesia): Soil Quality, Hydrological Protection of Adjacent Peatlands, and Sustainability
Authors: Silvianingsih Yosefin Ari , Hairiah Kurniatun , Suprayogo Didik , van Noordwijk Meine
Published: August 2021

Increased agricultural use of tropical peatlands has negative environmental effects. Drainage leads to landscape-wide degradation and fire risks. Livelihood strategies in peatland ecosystems have traditionally focused on transitions from riverbanks to peatland forests. Riparian ‘Kaleka’ agroforests with more than 100 years of history persist in the peatlands of Central Kalimantan (Indonesia), where large-scale open-field agricultural projects have dramatically failed. Our field study in a Dayak Ngaju village on the Kahayan river in the Pulang Pisau district involved characterizing land uses, surveying vegetation, measuring soil characteristics, and monitoring groundwater during a period of 16 months. We focused on how local practices and farmer knowledge compare with standard soil fertility (physical, chemical, biological) measurements to make meaningful assessments of risks and opportunities for sustainable land use within site-specific constraints. The Kaleka agroforests around a former settlement and sacred historical meaning are species-rich agroforests dominated by local fruit trees and rubber close to the riverbank. They function well with high wet-season groundwater tables (up to −15 cm) compatible with peatland restoration targets. Existing soil quality indices rate the soils, with low soil pH and high Alexch, as having low suitability for most annual crops, but active tree regeneration in Kaleka shows sustainability.
Evolution of Habitat Quality and Its Topographic Gradient Effect in Northwest Hubei Province from 2000 to 2020 Based on the InVEST Model
Authors: Li Mengyao , Zhou Yong , Xiao Pengnan , Tian Yang , Huang He , Xiao Liang
Published: August 2021

Regional land use change and ecological security are important fields and have been popular issues in global change research in recent years. Regional habitat quality is also an important embodiment of the service function and health of ecosystems. Taking Shiyan City of Hubei Province as an example, the spatiotemporal differences in habitat quality in Shiyan City were evaluated using the habitat quality module of the InVEST model and GIS spatial analysis method based on DEM and land use data from 2000, 2005, 2010, 2015, and 2020. According to the habitat quality index values, the habitats were divided into four levels indicating habitat quality: I (very bad), II (bad), III (good), and IV (excellent), and the topographic gradient effect of habitat quality was studied using the topographic position index. The results show the following. (1) The habitat quality of Shiyan City showed relatively high and obvious spatial heterogeneity overall and, more specifically, was high in the northwest and southwest, moderate in the center, and low in the northeast. The higher quality habitats (levels III, IV) were mainly distributed in mountain and hill areas and water areas, while those with lower quality habitats (levels I, II) were mainly distributed in agricultural urban areas. (2) From 2000 to 2020, the overall average habitat quality of Shiyan City first increased, then decreased, and then increased again. Additionally, the habitat area increased with an improvement in the level. There was a trend in habitat transformation moving from low to high quality level, showing a spatial pattern of “rising in the southwest and falling in the northeast”. (3) The habitat quality in the water area and woodland area was the highest, followed by grassland, and that of cultivated land was the lowest. From 2000 to 2020, the habitat quality of cultivated land, woodland, and grassland decreased slightly, while the habitat quality of water increased significantly. (4) The higher the level of the topographic position index, the smaller the change range of land use types with time. The terrain gradient effect of habitat quality was significant. With the increase in terrain level, the average habitat quality correspondingly improved, but the increasing range became smaller and smaller. These results are helpful in revealing the spatiotemporal evolution of habitat quality caused by land use changes in Shiyan City and can provide a scientific basis for the optimization of regional ecosystem patterns and land use planning and management, and they are of great significance for planning the rational and sustainable use of land resources and the construction of an ecological civilization.
Research on Identification of Multiple Cropping Index of Farmland and Regional Optimization Scheme in China Based on NDVI Data
Authors: Li Tingting , Wang Yanfei , Liu Changquan , Tu Shuangshuang
Published: August 2021

The multiple cropping index of farmland is a significant characterization of land use intensity. Based on the NDVI data, this paper calculated the multiple cropping index of farmland in China using the S-G filtering method, and proposed an optimized regionalization scheme for the farmland use. The findings reveal that from 2000 to 2018, the multiple cropping index of farmland in China underwent the fluctuation of rising first, then falling and rising continuously, which was closely associated with the agricultural support policies enforced in China. Counties whose multiple cropping indexes decreased from 2009 to 2018 were mainly located in areas primarily producing grain, which exerted a greater influence on food security. The gap between the multiple cropping index and potential multiple cropping index of farmland is increasingly widening from north to south in China. Accordingly, four types of grain producing zones were delineated: key development zone, potential growth zone, appropriate development zone, and restricted development zone. Some suggestions, such as rotation, fallow, determination of yield by water and offsetting the quantity balance of farmland by increasing the multiple cropping index, are put forward based on different zones.
Identification of Autophagy- and Ferroptosis-Related lncRNAs Functioned through Immune-Related Pathways in Head and Neck Squamous Carcinoma
Authors: Guo Qi , Zhang Xuehan , Shen Tao , Wang Xiangting
Published: August 2021

The interplay between autophagy and ferroptosis has been highlighted as an important event to decide cancer cell fate. However, the underlying mechanisms remain largely unclear. In this study, we systematically explored the expression, prognostic value and functional roles of lncRNA in autophagy and ferroptosis. By a set of bioinformatics analyses, we identified 363 autophagy- and ferroptosis-related lncRNAs (AF-lncRNAs) and found 17 of them are dramatically related to the prognosis of head and neck squamous cell carcinoma (HNSC) patients, named as prognosis-related AF-lncRNAs (PAF-lncRNAs). Based on six key PAF-lncRNAs, a risk score model was developed and used to categorize the TCGA-retrieved HNSC patients into two groups (high-risk vs. low-risk). Functional analysis showed the differentially expressed genes (DEGs) between the two groups were mainly enriched in immune-related pathways and regulated by a PAF-lncRNA-directed ceRNA (competitive endogenous RNA) network. Combined with a variety of immune infiltration analyses, we also found a decreased landscape of immune cell infiltration in high-risk groups. Together, by revealing PAF-lncRNAs with tumor prognostic features functioned through immune-related pathways, our work would contribute to show the pathogenesis of a lncRNA-directed interplay among autophagy, ferroptosis and tumor immunity in HNSC and to develop potential prognostic biomarkers and targets for tumor immunotherapy.
Roles of AMPK and Its Downstream Signals in Pain Regulation
Authors: Wang Shenglan , Dai Yi
Published: August 2021

Pain is an unpleasant sensory and emotional state that decreases quality of life. A metabolic sensor, adenosine monophosphate-activated protein kinase (AMPK), which is ubiquitously expressed in mammalian cells, has recently attracted interest as a new target of pain research. Abnormal AMPK expression and function in the peripheral and central nervous systems are associated with various types of pain. AMPK and its downstream kinases participate in the regulation of neuron excitability, neuroinflammation and axonal and myelin regeneration. Numerous AMPK activators have reduced pain behavior in animal models. The current understanding of pain has been deepened by AMPK research, but certain issues, such as the interactions of AMPK at each step of pain regulation, await further investigation. This review examines the roles of AMPK and its downstream kinases in neurons and non-neuronal cells, as well as their contribution to pain regulation.
Phytochemical and Biological Screening of Leaf, Bark and Fruit Extracts from Ilex dipyrena Wall.
Authors: Ali Amjad , Khalil Atif Ali Khan , Khuda Fazli , Nazir Nausheen , Ullah Riaz , Bari Ahmed , Haider Adnan , Jamal Syed Babar , Ahmad Sareer , Khan Zahid , Shah Syed Muhammad Mukarram , Shah Syed Wadood Ali , Nasir Abdul , Ali Abid , Zahoor Muhammad , Jan Samin
Published: August 2021

The Aquifoliaceae is an important family and has been used traditionally for some time. One of the members of this family is the Ilex dipyrena wall, which itself possesses a potential medicinal importance. This plant is traditionally used for the treatment of various ailments including pain, swelling, burns, and fever. The current study was designed to screen out the antioxidant and analgesic potential of this plant and to verify its traditional uses, along with its phytochemical profile. Extracts were subjected to antioxidant, analgesic, and phytochemical analysis using DPPH, chemical-induced (acetic acid and formalin) nociception models and GC-MS analysis, respectively. The leaf, bark, and fruit extracts showed significant antioxidant activity compared to that of standard. Likewise, all the extracts demonstrated significant (p < 0.01) analgesic activity in a mice model. In acetic acid induced analgesia, the leaf, bark, and fruit extracts caused 51.64, 56.13 and 59.52% inhibition, respectively at a dose of 100 mg/kg while at 200 mg/kg it showed 83.01, 71.69 and 75.47% inhibition, respectively. In Formalin-induced paw-licking assay, fruit extract showed 59.42 and 64.19% inhibition at 200 mg/kg dose in the first and second phase, respectively. The GC-MS analysis revealed the presence of cathinone, phenylpropanolamine, dl-phenylephrine, amphetamine, myristic acid, and palmitic acid. Results of the study suggest that crude extracts from different parts of this plant may be a useful source for the development of novel analgesics. However, further investigation in terms of isolation of bioactive compounds and their toxicological evaluations are needed to validate the observed results.
Towards Next Generation Biomarkers in Natural Killer/T-Cell Lymphoma
Authors: Chan Jason Yongsheng , Lim Jing Quan , Ong Choon Kiat
Published: August 2021

Natural killer/T-cell lymphoma (NKTCL) is an Epstein–Barr virus-associated non-Hodgkin lymphoma linked to an aggressive clinical course and poor prognosis. Despite an improvement in survival outcomes with the incorporation of novel agents including immune checkpoint inhibitors in the treatment of NKTCL, a significant proportion of patients still relapse or remain refractory to treatment. Several clinical prognostic models have been developed for NKTCL patients treated in the modern era, though the optimal approach to risk stratification remains to be determined. Novel molecular biomarkers derived from multi-omic profiling have recently been developed, with the potential to improve diagnosis, prognostication and treatment of this disease. Notably, a number of potential biomarkers have emerged from a better understanding of the tumor immune microenvironment and inflammatory responses. This includes a recently described 3′UTR structural variant in the PD-L1 gene, which confers susceptibility to checkpoint immunotherapy. In this review, we summarize the biomarker landscape of NKTCL and highlight emerging biomarkers with the potential for clinical implementation.
Improvements of the MQL Cooling-Lubrication Condition by the Addition of Multilayer Graphene Platelets in Peripheral Grinding of SAE 52100 Steel
Authors: Abrão Bruno Souza , Pereira Mayara Fernanda , da Silva Leonardo Rosa Ribeiro , Machado Álisson Rocha , Gelamo Rogério Valentim , de Freitas Fábio Martinho Cézar , Mia Mozammel , da Silva Rosemar Batista
Published: August 2021

In most grinding processes, the use of cutting fluid is required, and research has been carried out to reduce the amount of fluid used due to costs and environmental impacts. However, such a reduction of fluid can result in thermal damage to the machined component because the amount of cutting fluid may not be sufficient to lubricate and cool the system. One way of improving the cutting fluid properties is to add micro or nanoparticles of solid lubricants. This paper aims to evaluate the performance of multilayer graphene platelets dispersed in cutting fluid and applied through the technique of minimum quantity of lubrication (MQL) during the peripheral surface grinding of SAE 52100 hardened steel. In this sense, the influence of these solid particles with respect to the surface and sub-surface integrity of the machined components was analyzed, performing the roughness and microhardness measurement and analyzing the ground surfaces. The results showed that the cooling–lubrication conditions employing graphene could obtain smaller roughness values and decreases of microhardness in relation to the reference value and components with better surface texture compared to the conventional MQL technique without solid particles.
The Stability Analysis of a Double-X Queuing Network Occurring in the Banking Sector
Authors: Zhang Hong , Akuamoah Saviour Worlanyo , Apeanti Wilson Osafo , Harvim Prince , Yaro David , Georgescu Paul
Published: August 2021

We model a common teller–customer interaction occurring in the Ghanaian banking sector via a Double-X queuing network consisting of three single servers with infinite-capacity buffers. The servers are assumed to face independent general renewal of customers and independent identically distributed general service times, the inter-arrival and service time distributions being different for each server. Servers, when free, help serve customers waiting in the queues of other servers. By using the fluid limit approach, we find a sufficient stability condition for the system, which involves the arrival and service rates in the form of a set of inequalities. Finally, the model is validated using an illustrative example from a Ghanaian bank.
DMO-QPSO: A Multi-Objective Quantum-Behaved Particle Swarm Optimization Algorithm Based on Decomposition with Diversity Control
Authors: You Qi , Sun Jun , Pan Feng , Palade Vasile , Ahmad Bilal
Published: August 2021

The decomposition-based multi-objective evolutionary algorithm (MOEA/D) has shown remarkable effectiveness in solving multi-objective problems (MOPs). In this paper, we integrate the quantum-behaved particle swarm optimization (QPSO) algorithm with the MOEA/D framework in order to make the QPSO be able to solve MOPs effectively, with the advantage of the QPSO being fully used. We also employ a diversity controlling mechanism to avoid the premature convergence especially at the later stage of the search process, and thus further improve the performance of our proposed algorithm. In addition, we introduce a number of nondominated solutions to generate the global best for guiding other particles in the swarm. Experiments are conducted to compare the proposed algorithm, DMO-QPSO, with four multi-objective particle swarm optimization algorithms and one multi-objective evolutionary algorithm on 15 test functions, including both bi-objective and tri-objective problems. The results show that the performance of the proposed DMO-QPSO is better than other five algorithms in solving most of these test problems. Moreover, we further study the impact of two different decomposition approaches, i.e., the penalty-based boundary intersection (PBI) and Tchebycheff (TCH) approaches, as well as the polynomial mutation operator on the algorithmic performance of DMO-QPSO.
TACI Mutations in Primary Antibody Deficiencies: A Nationwide Study in Greece
Authors: Kakkas Ioannis , Tsinti Gerasimina , Kalala Fani , Farmaki Evangelia , Kourakli Alexandra , Kapousouzi Androniki , Dimou Maria , Kalaitzidou Vassiliki , Sevdali Eirini , Peristeri Athanasia-Marina , Tsiouma Georgia , Patiou Peristera , Papadimitriou Eleni , Vassilakopoulos Theodoros P. , Panayiotidis Panayiotis , Kioumi Anna , Symeonidis Argiris , Speletas Matthaios
Published: August 2021

Background and objectives: Monoallelic (heterozygous) or biallelic (homozygous or compound heterozygous) TACI mutations have been reported as the most common genetic defects in patients with common variable immunodeficiency (CVID), which is the most common clinically significant primary immunodeficiency in humans. The aim of our study was to evaluate the prevalence and any correlations of TACI defects in Greek patients with primary antibody deficiencies. Materials and Methods: 117 patients (male/female: 53/64) with CVID (110) and a combined IgA and IgG subclass deficiency (7) with a CVID-like clinical phenotype were enrolled in the study. Genomic DNA was extracted from peripheral blood and the molecular analysis of the TACI gene was performed by PCR (Polymerase Chain Reaction) and sequencing of all 5 exons, including exon–intron boundaries. Results: Seventeen patients (14.5%) displayed TACI defects, four (23.5%) carried combined heterozygous mutations and 13 (76.5%) carried single heterozygous mutations. The most frequently detected mutation was C104R (58.8%), followed by I87N (23.5%) and A181E (11.8%), while R20C, C62Y, P151L, K188M and E236X mutations were present in only one patient each. Patients with TACI defects were more frequently male (p = 0.011) and displayed a benign lymphoproliferation (splenomegaly and lymph node enlargement, p = 0.047 and p = 0.002, respectively), had a history of tonsillectomy (p = 0.015) and adenoidectomy (p = 0.031) and more frequently exhibited autoimmune cytopenias (p = 0.046). Conclusions: Considering that accumulating evidence suggests several CVID patients have a complex rather than a monogenic inheritance, our data further support the notion that TACI mutations, particularly as monoallelic defects, should be primarily considered as susceptibility co-factors and/or modifiers of primary antibody deficiencies.
Erythema Multiforme and COVID-19: What Do We Know?
Authors: Bennardo Luigi , Nisticò Steven Paul , Dastoli Stefano , Provenzano Eugenio , Napolitano Maddalena , Silvestri Martina , Passante Maria , Patruno Cataldo
Published: August 2021

Background: Erythema multiforme (EM) is an acute cutaneous eruption often associated with infections and more rarely with drugs. This review aimed to evaluate the association between erythema multiforme and coronavirus disease 2019 (COVID-19). Methods: A systematic search of PubMed/MEDLINE, Scimago Scopus, and ISI/Web of Science was performed. Original articles, case series, or case reports were evaluated and selected. Results: Fourteen articles were selected, describing a total of 70 patients. EM is a cutaneous eruption rarely occurring in COVID-19 and is, in most cases, associated with a hypersensitivity reaction to the virus. In these cases, EM seems to affect patients younger than 30 years or older than 55 years. Infrequently, some drugs used in the management of COVID-19 may induce EM, especially hydroxychloroquine. The three groups of patients seem to have different clinical characteristics and courses. Conclusions: From these data, it is possible to preliminarily propose that EM or EM-like eruptions linked to COVID-19 might be divided into three types: the virus-related juvenile type (affecting patients <30-year-old), the virus-related older type (affecting patients >55 years), and the drug-induced type. The occurrence of a skin rash does not seem to be related to the severity and clinical course of COVID-19.
Particle Size Distribution Models for Metallurgical Coke Grinding Products
Authors: Colorado-Arango Laura , Menéndez-Aguado Juan M. , Osorio-Correa Adriana
Published: August 2021

Six different particle size distribution (Gates–Gaudin–Schuhmann (GGS), Rosin–Rammler (RR), Lognormal, Normal, Gamma, and Swebrec) models were compared under different metallurgical coke grinding conditions (ball size and grinding time). Adjusted R2, Akaike information criterion (AIC), and the root mean of square error (RMSE) were employed as comparison criteria. Swebrec and RR presented superior comparison criteria with the higher goodness-of-fit and the lower AIC and RMSE, containing the minimum variance values among data. The worst model fitting was GGS, with the poorest comparison criteria and a wider results variation. The undulation Swebrec parameter was ball size and grinding time-dependent, considering greater b values (b > 3) at longer grinding times. The RR α parameter does not exhibit a defined tendency related to grinding conditions, while the k parameter presents smaller values at longer grinding times. Both models depend on metallurgical coke grinding conditions and are hence an indication of the grinding behaviour. Finally, oversize and ultrafine particles are found with ball sizes of 4.0 cm according to grinding time. The ball size of 2.54 cm shows slight changes in particle median diameter over time, while 3.0 cm ball size requires more grinding time to reduce metallurgical coke particles.
Effect of Cooling Rates on the Local—Overall Morphology Characteristics of Solidification Structure at Different Stages for High Carbon Steel
Authors: Cao Jianghai , Zeng Zihang , Zhang Fuli , Guo Dongwei , Hou Zibing
Published: August 2021

The solidification characteristics of 70 steel at the stage of the superheat elimination and the liquid–solid phase transformation were analyzed at cooling rates from 10 to 150 °C/min based on a high-temperature confocal scanning laser microscope (HT-CSLM). Secondary dendrite arm spacing (SDAS) and fractal dimension (D) were used to quantitatively describe the local compactness and overall self-similar complexity of the solidification morphology. It was found that the cooling rate had a very important influence on the local and overall morphology characteristics of solidification structures. At the superheat elimination stage, the cooling rate affected the morphology of the microstructure through the dynamic structural fluctuation between the generation and disappearance of atomic clusters in the molten steel. At the liquid–solid phase transformation stage, the cooling rate affected the local morphology of the microstructure by affecting the solute diffusion rate between dendrite arms, while it affected the overall morphology by changing the concentration undercooling at the front of all solidified interfaces. The presented results show that adjusting the cooling system at the superheat elimination stage can also be an important way to control the solidified morphology of different alloys.
Evaluation of the Effect of Minor Additions in the Crystallization Path of [(Fe0.5Co0.5)0.75B0.2Si0.05]100-xMx Metallic Glasses by Means of Mössbauer Spectroscopy
Authors: Panahi S. Leila , Ramasamy Parthiban , Masdeu Francesc , Stoica Mihai , Torrens-Serra Joan , Bruna Pere
Published: August 2021

Understanding the crystallization of metallic glasses is fundamental in the design of new alloys with enhanced properties and better glass-formability. The crystallization of a series of Fe-based metallic glasses of composition [(Fe0.5Co0.5)0.75B0.2Si0.05]100-xMx (M = Mo, Nb and Zr) has been studied by means of differential scanning calorimetry and transmission Mössbauer spectroscopy. This latter technique allows the following of the microstructural evolution of the studied alloys through the identification and quantification of the several Fe-containing crystalline phases and also through the changes in the amorphous structure at the initial stages of crystallization. The results show that the crystallization products are the same for all the studied compositions (α-Fe, Fe2B, (FeCo)23B6 and a paramagnetic remnant) although with different relative proportions and the crystallization of a phase without Fe in the alloys with Zr. Moreover, the addition of Zr favors the crystallization of α-Fe causing a detrimental effect on the glass forming ability, while the increase in Mo content up to 6 at% favors the crystallization of (FeCo)23B6. The different amount of α-Fe and borides is presented as a measure of the glass forming ability of this type of alloys.
Effect of MgO Content on Heat Capacity of Synthetic BF Slag and Heat Release Behavior during Cooling Process
Authors: Yang Yanhua , Lei Ting , Qin Yuelin
Published: August 2021

The differential scanning calorimetry (DSC) sapphire analysis was used to measure the specific heat capacity of the BF (BF) slag and observe the CaO-SiO2-MgO-Al2O3-TiO2 5-element slag system with the binary basicity fixed at 1.17. The specific heat capacity of the BF slag and the cooling heat distribution were obtained during the cooling process when the MgO content changing from 7% to 11%. The results showed that the heat released of BF slag was more than 1.2 GJ/ton during the cooling process from 1400 °C to 35 °C, of which the sensible heat was dominant. At MgO content of 9%, the latent heat of crystallization is maximum. The cooling and heat release law of BF slag is directly associated with the phase precipitated in slag cooling and micromorphology.
Composition-Induced Magnetic Transition in GdMn1-xTixSi Intermetallic Compounds for x = 0–1
Authors: Mukhachev Roman D. , Lukoyanov Alexey V.
Published: August 2021

Magnetic intermetallic compounds based on rare earth elements and 3d transition metals are widely investigated due to the functionality of their physical properties and their variety of possible applications. In this work, we investigated the features of the electronic structure and magnetic properties of ternary intermetallic compounds based on gadolinium GdMn1-xTixSi, in the framework of the DFT + U method. Analysis of the densities of electronic states and magnetic moments of ions in Ti-doped GdMnSi showed a significant change in the magnetic properties depending on the contents of Mn and Ti. Together with the magnetic moment, an increase in the density of electronic states at the Fermi energy was found in almost all GdMn1-xTixSi compositions, which may indicate a significant change in the transport properties of intermetallic compounds. Together with the expected Curie temperatures above 300 K, the revealed changes in the magnetic characteristics and electronic structure make the GdMn1-xTixSi intermetallic system promising for use in microelectronic applications.
Clinical Outcomes of Immunocompromised Adults Hospitalized with Pneumococcal Pneumonia: A Case-Control Study
Authors: Ramirez Julio , Chandler Thomas , Furmanek Stephen , Arnold Forest , Bordon Jose
Published: August 2021

S. pneumoniae is a primary etiologic agent of CAP in immunocompromised adults (ICA). Data on clinical outcomes of ICA hospitalized with pneumococcal pneumonia (PP) is limited. The objectives of this study were (1) to define clinical presentation and outcomes of ICA hospitalized with PP and (2) to compare the data to non-immunocompromised adults (non-ICA) hospitalized with PP. This was a case–control study of ICA hospitalized with PP (cases) and non-ICA hospitalized with PP (controls). Data were collected on clinical presentation, treatment, and outcomes. Evaluated clinical outcomes included time to clinical stability (TCS), length of hospitalization (LOH), clinical failure (CF), cardiovascular events (CE), and in-hospital mortality (IHM). One ICA was matched to two non-ICA through propensity score matching. A total of 93 ICA hospitalized with PP and 186 non-ICA hospitalized with PP were evaluated. Antibiotic therapy was appropriate in all patients. Clinical outcomes for ICA versus non-ICA were as follows: TCS 2 days vs. 2 days (p = 0.392); LOH 5 days vs. 5 days (p = 0.067); CF 4% vs. 6% (p = 0.618); CE 10% vs. 6% (p = 0.375); and IHM 5% vs. 3% (p = 0.296). In hospitalized patients with PP who are treated with appropriate antibiotic therapy, the presence of an abnormal immune system does influence clinical outcomes.
Aphrophoridae Role in Xylella fastidiosa subsp. pauca ST53 Invasion in Southern Italy
Authors: Picciotti Ugo , Lahbib Nada , Sefa Valdete , Porcelli Francesco , Garganese Francesca
Published: August 2021

The Philaenus spumarius L. (Hemiptera Aphrophoridae) is a xylem-sap feeder vector that acquires Xylella fastidiosa subsp. pauca ST53 during feeding on infected plants. The bacterium is the plant pathogen responsible for olive quick decline syndrome that has decimated olive trees in Southern Italy. Damage originates mainly from the insect vector attitude that multiplies the pathogen potentialities propagating Xf in time and space. The principal action to manage insect-borne pathogens and to contain the disease spread consists in vector and transmission control. The analysis of an innovative and sustainable integrated pest management quantitative strategy that targets the vector and the infection by combining chemical and physical control means demonstrates that it is possible to stop the Xylella invasion. This review updates the available topics addressing vectors’ identification, bionomics, infection management, and induced disease by Xylella invasion to discuss major available tools to mitigate the damage consequent to the disease.
Recombinant SARS-CoV-2 Nucleocapsid Protein: Expression, Purification, and Its Biochemical Characterization and Utility in Serological Assay Development to Assess Immunological Responses to SARS-CoV-2 Infection
Authors: Di Da , Dileepan Mythili , Ahmed Shamim , Liang Yuying , Ly Hinh
Published: August 2021

The SARS-CoV-2 nucleocapsid protein (N) binds a single-stranded viral RNA genome to form a helical ribonucleoprotein complex that is packaged into virion particles. N is relatively conserved among coronaviruses and consists of the N-terminal domain (NTD) and C-terminal domain (CTD), which are flanked by three disorganized regions. N is highly immunogenic and has been widely used to develop a serological assay as a diagnostic tool for COVID-19 infection, although there is a concern that the natural propensity of N to associate with RNA might compromise the assay’s specificity. We expressed and purified from bacterial cells two recombinant forms of SARS-CoV-2 N, one from the soluble fraction of bacterial cell lysates that is strongly associated with bacterial RNAs and the other that is completely devoid of RNAs. We showed that both forms of N can be used to develop enzyme-linked immunosorbent assays (ELISAs) for the specific detection of human and mouse anti-N monoclonal antibodies (mAb) as well as feline SARS-CoV-2 seropositive serum samples, but that the RNA-free form of N exhibits a slightly higher level of sensitivity than the RNA-bound form to react to anti-N mouse mAb. Using the electrophoretic mobility shift assay (EMSA), we also showed that N preferentially binds ssRNA in a sequence-independent manner and that both NTD and CTD of N contribute to RNA-binding activity. Collectively, our study describes methods to express, purify, and biochemically characterize the SARS-CoV-2 N protein and to use it for the development of serological assays to detect SARS-CoV-2 infection.
Optimization of DNA Extraction from Field-Collected Mammalian Whole Blood on Filter Paper for Trypanosoma cruzi (Chagas Disease) Detection
Authors: Gulas-Wroblewski Bonnie E. , Kairis Rebecca B. , Gorchakov Rodion , Wheless Anna , Murray Kristy O.
Published: August 2021

Blood filter paper strips are cost-effective materials used to store body fluid specimens under challenging field conditions, extending the reach of zoonotic pathogen surveillance and research. We describe an optimized procedure for the extraction of parasite DNA from whole blood (WB) stored on Type I Advantec Nobuto strips from both experimentally spiked and field-collected specimens from canine and skunks, respectively. When comparing two commercial kits for extraction, Qiagen’s DNeasy Blood & Tissue Kit performed best for the detection of parasite DNA by PCR from Trypanosoma cruzi-spiked canine WB samples on Nobuto strips. To further optimize recovery of β-actin from field-collected skunk WB archived on Nobuto strips, we modified the extraction procedures for the Qiagen kit with a 90 °C incubation step and extended incubation post-addition of proteinase K, a method subsequently employed to identify a T. cruzi infection in one of the skunks. Using this optimized extraction method can efficaciously increase the accuracy and precision of future molecular epidemiologic investigations targeting neglected tropical diseases in field-collected WB specimens on filter strips.