bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of the Destruction Model of Ventilated Facade under the Influence of Fire
Authors: Schabowicz Krzysztof , Sulik Paweł , Zawiślak Łukasz
Published: May 2020

Ventilated facades are becoming an increasingly popular solution for external part of walls in the buildings. They may differ in many elements, among others things: claddings (fiber cement boards, HPL plates, large-slab ceramic tiles, ACM panels, stone cladding), types of substructures, console supports, etc. The main part that characterizes ventilated facades is the use of an air cavity between the cladding and thermal insulation. Unfortunately, in some aspects they are not yet well-standardized and tested. Above all, the requirements for the falling-off of elements from ventilated facades during a fire are not precisely defined by, among other things, the lack of clearly specified requirements and testing. This is undoubtedly a major problem, as it significantly affects the safety of evacuation during a fire emergency. For the purposes of this article, experimental tests were carried out on a large-scale facade model, with two types of external-facade cladding. The materials used as external cladding were fiber cement boards and large-slab ceramic tiles. The model of large-scale test was 3.95 m × 3.95 m, the burning gas released from the burner was used as the source of fire. The test lasted one hour. The facade model was equipped with thermocouples. The cladding materials showed different behavior during the test. Large-slab ceramic tiles seemed to be a safer form of external cladding for ventilated facades. Unfortunately, they were destroyed much faster, for about 6 min. Large-slab ceramic tiles were destroyed within the first dozen or so minutes, then their destruction did not proceed or was minimal. In the case of fiber cement boards, the destruction started from the eleventh minute and increased until the end of the test. The authors referred the results of large-scale test to testing on samples carried out by other authors. The results presented the convergence of large-scale test with samples. External claddings was equipped with additional mechanical protection. The use of additional mechanical protection to maintain external cladding elements increases their safety but does not completely eliminate the problem of the falling-off of parts of the facade. As research on fiber cement boards and large-slab ceramic tiles presented, these claddings were a major hazard due to fall-off from facade.
Clinical and Radiographic Evaluation of Simultaneous Alveolar Ridge Augmentation by Means of Preformed Titanium Meshes at Dehiscence-Type Peri-Implant Defects: A Prospective Pilot Study
Authors: Maiorana Carlo , Manfredini Mattia , Beretta Mario , Signorino Fabrizio , Bovio Andrea , Poli Pier Paolo
Published: May 2020

Background: bone augmentation by means of manually shaped titanium mesh is an established procedure to regenerate atrophic alveolar ridges and recreate a proper contour of the peri-implant bone anatomy. Conversely, current literature on the use of preformed titanium meshes instead of traditional grids remains lacking. Therefore, the aim of the present prospective study was to evaluate the use of preformed titanium mesh to support bone regeneration simultaneously to implant placement at dehiscence-type defects from clinical, radiological, and patient-related outcomes. Methods: 8 implants showing buccal dehiscence defects were treated with preformed titanium mesh directly fixed to flat abutments screwed to the implant. Intrasurgical clinical measurements and radiographic evaluations by means of cone-beam computed tomography scans were performed to assess the horizontal bone gain after 8 months from the augmentation surgery. Biological and patient-centered outcomes were also evaluated.; Results: clinically, a mean horizontal bone gain of 4.95 ± 0.96 mm, and a mean horizontal thickness of the buccal plate of 3.25 ± 0.46 mm were found. A mean horizontal bone gain of 5.06 ± 0.88 mm associated with a mean horizontal thickness of the buccal plate of 3.45 ± 0.68 mm were observed radiographically. From a macroscopic aspect, the remodeled graft appeared well integrated with the host bone. Well vascularized newly formed bone-like tissue was observed in intimate contact with the implants. Conclusions: preformed titanium mesh may be effective in supporting simultaneous horizontal bone regeneration at dehiscence-type peri-implant defects. Titanium mesh exposure still remain an issue in this type of surgery.
Influence of Phosphorus Slag on Physical and Mechanical Properties of Cement Mortars
Authors: Pang Min , Sun Zhenping , Chen Ming , Lang Jianlei , Dong Jiayan , Tian Xu , Sun Jiliang
Published: May 2020

Influences of phosphorus slag from 10% to 50% (by mass) on the setting time and the water requirement of the normal consistency of cement pastes, flowability, resistance to carbonation, and the compressive strength of cement mortars were investigated. The physical activation by improving fineness and the chemical activation by adding the chemical activator were evaluated by the compressive strength of cement mortars with 30% by mass of phosphorus slag. Hydration heat, X-ray diffraction, and scanning electron microscopy were used to study the microstructure of cement pastes and mortars with 30% by mass of phosphorus slag and the chemical activator. Results showed that the setting time of cement pastes was delayed by phosphorus slag from 10% to 50%. Phosphorus slag had nearly no effects on the water requirement of the normal consistency of cement pastes and the flowability of cement mortars. The resistance to carbonation of cement mortars was decreased by phosphorus slag from 10% to 50% according to the acceleration carbonation. The compressive strength of cement mortars was also decreased by phosphorus slag from 10% to 50% and the low activity of phosphorus slag was concluded based on compressive strength of cement mortars. The effect of the chemical activator on the compressive strength of cement mortars with 30% by mass of phosphorus slag was better than improving fineness of phosphorus slag from 300 m2/kg to 450 m2/kg. Both hydration heat and cement hydrates were inhibited by phosphorus slag and could be partly compensated by the chemical activator. Loose morphology and propagations of microcracks were found in cement pastes and mortars with 30% by mass of phosphorus slag.
The Influences of Moisture on the Mechanical, Morphological and Thermogravimetric Properties of Mineral Wool Made from Basalt Glass Fibers
Authors: Ivanič Andrej , Kravanja Gregor , Kidess Wadie , Rudolf Rebeka , Lubej Samo
Published: May 2020

Mineral wool made from basalt fibers is frequently used as an insulating material in construction systems. In this study, both unused mineral wool and wool obtained from the softened roofing area were comprehensively analyzed in a laboratory using different characterization techniques. Firstly, the initial water content and compressive strength at 10% deformation were determined. Secondly, microstructure and surface chemical composition were analyzed by scanning electron microscopy (SEM) equipped with energy dispersive X-ray spectroscopy (EDX). To study heterogeneities near the fiber surface and to examine cross-sectional composition, a scanning transmission electron microscope (STEM) was used. Finally, to verify possible reasons for resin degradation, thermogravimetric analysis and differential scanning colometry (TGA-DSC) were simultaneously carried out. The results show that natural aging under high humidity and thermal fluctuations greatly affected the surface morphology and chemical composition of the fibrous composite. Phenol-formaldehyde and other hydrophobic compounds that protect fibers against moisture and give compressive resistance were found to be degraded.
Study of Carbon Black Types in SBR Rubber: Mechanical and Vibration Damping Properties
Authors: Pöschl Marek , Vašina Martin , Zádrapa Petr , Měřínská Dagmar , Žaludek Milan
Published: May 2020

Styrene–butadiene rubber mixtures with four types of carbon black were studied in this paper. The mechanical properties, including the ability to damp mechanical vibration, were investigated, along with dynamical mechanical analysis (DMA). It has been found that carbon black types N 110 and N 330, having the largest specific surface area and the smallest particle diameter, provide a good stiffening effect. These particles have significant interactions between the rubber, resulting in good reinforcement. On the other hand, the carbon black N 990 type has a lower reinforcing effect and improved vibration damping properties at higher excitation frequencies due to higher dissipation of mechanical energy into heat under dynamic loading. The effect of the number of loading cycles on vibration damping properties of the rubber composites was also investigated in this study. It can be concluded that the abovementioned properties of the investigated rubber composites correspond to physical–mechanical properties of the applied carbon black types.
Experimental Studies on Durability of PVD-Based CrCN/CrN-Coated Cutting Blade of Planer Knives Used in the Pine Wood Planing Process
Authors: Nadolny Krzysztof , Kapłonek Wojciech , Sutowska Marzena , Sutowski Paweł , Myśliński Piotr , Gilewicz Adam
Published: May 2020

The condition of the cutting tool is one of the most important factors as it directly affects the technological and economic efficiencies of the woodworking process. The large variety of raw materials of wood combined with possible impurities and inclusion of solids puts high demands on planing machines. One of the methods to modify their operational properties is to apply antiwear coating on their working surfaces using vacuum deposition methods, such as physical vapor deposition (PVD). The use of such coatings reduces the adhesion of planing products to the surface of industrial planer knives, reduces the friction between the cutting tool and the workpiece, and limits the penetration of heat into the tool, thereby contributing to extending its effective working life. This study examines the impact of PVD-based CrCN/CrN coating on the operational durability and intensity of wear of planer knives operating in production conditions compared to unmodified knives (typically used in the wood processing industry for pine wood planing). For the unmodified and CrCN/CrN-coated planing blades (before and after processing), detailed analyses were carried out. These analyses included determining the rounding radius and profile along the blade (worn edge displacement), calculating surface texture parameters of the rake face of planer knives, and carrying out visual microscopic analysis of its condition. The results of the experiments indicated an increase in durability of up to 142% for the CrCN/CrN-coated tools. It was also found that the use of PVD-based modified industrial planer knives turned out to be more beneficial in each analyzed area of analysis.
On the Core-Shell Nanoparticle in Fractional Dimensional Space
Authors: Ali A. , Ashraf M. A. , Minhas Q. A. , Naqvi Q. A. , Baqir M. A. , Choudhury P. K.
Published: May 2020

The investigation of core-shell nanoparticles has been greatly exciting in biomedical applications, as this remains of prime importance in targeted drug delivery, sensing, etc. In the present work, the polarizability and scattering features of nanoparticles comprised of nano-sized dielectric/metallic core-shell structures were investigated in the fractional dimensional (FD) space, which essentially relates to the confinement of charged particles. For this purpose, three different kinds of metals—namely aluminum, gold and silver—were considered to form the shell, having a common silicon dioxide (SiO2) nanoparticle as the core. It is noteworthy that the use of noble metal-SiO2 mediums interface remains ideal to realize surface plasmon resonance. The core-shell nanoparticles were considered to have dimensions smaller than the operating wavelength. Under such conditions, the analyses of polarizability and the scattering and absorption cross-sections, and also, the extinction coefficients were taken up under Rayleigh scattering mechanism, emphasizing the effects of a varying FD parameter. Apart from these, the tuning of resonance peaks and the magnitude of surface plasmons due to FD space parameter were also analyzed. It was found that the increase of FD space parameter generally results in blue-shifts in the resonance peaks. Apart from this, the usage of gold and silver shells brings in fairly large shifts in the peak positions of wavelengths, which allows them to be more suitable for a biosensing purpose.
The Preliminary Exploration of Micro-Friction Stir Welding Process and Material Flow of Copper and Brass Ultra-Thin Sheets
Authors: Zhang Changqing , Qin Zhuo , Rong Chen , Shi Wenchen , Wang Shuwen
Published: May 2020

In the friction stir welding (FSW) of ultra-thin dissimilar metal sheets, different physical material properties, the reduction of plastic metal in the weld zone, and insufficient plastic metal flow lead to poor weld seam shapes and joint qualities. Therefore, it is necessary to study the flow behavior during the FSW of ultrathin sheets. In this study, micro friction stir welding (μFSW) was conducted and analyzed for the butt welding of 0.6-mm-thick ultrathin brass (H62-H) and pure copper (T2-Y) sheets. By analyzing the electric signals of the temperature and force during the welding process, testing the mechanical properties, and analyzing the metallography of the joint, the influences of the process parameters on the metal flow behavior during μFSW were studied. In the proper process conditions, the material preferentially migrated and concentric vortex flow occurred in the vicinity of the shoulder and tool pin action areas. The copper was pushed from the retreating side (RS) to the advancing side (AS) of the weld, allowing it to flow more fully. A mixture of both materials formed at the bottom of the weld nugget, and less migration occurred in the heat-affected zone of the AS at this time. The highest tensile strength can reach 194 MPa, accounting for 82.6% of the copper. The presence of brittle phases Cu5Zn8, AgZn3 and AgZn caused the hardness to fluctuate slightly.
On ℋ-Simulation Functions and Fixed Point Results in the Setting of ωt-Distance Mappings with Application on Matrix Equations
Authors: Bataihah Anwar , Shatanawi Wasfi , Qawasmeh Tariq , Hatamleh Raed
Published: May 2020

The concepts of b-metric spaces and ω t -distance mappings play a key role in solving various kinds of equations through fixed point theory in mathematics and other science. In this article, we study some fixed point results through these concepts. We introduce a new kind of function namely, H -simulation function which is used in this manuscript together with the notion of ω t -distance mappings to furnish for new contractions. Many fixed point results are proved based on these new contractions as well as some examples are introduced. Moreover, we introduce an application on matrix equations to focus on the importance of our work.
Interacting Ru(bpy)

3

2
+

Dye Molecules and TiO2 Semiconductor in Dye-Sensitized Solar Cells
Authors: Putthikorn Sasipim , Tran-Duc Thien , Thamwattana Ngamta , Hill James M. , Baowan Duangkamon
Published: May 2020

Solar energy is an alternative source of energy that can be used to replace fossil fuels. Various types of solar cells have been developed to harvest this seemingly endless supply of energy, leading to the construction of solar cell devices, such as dye-sensitized solar cells. An important factor that affects energy conversion efficiency of dye-sensitized solar cells is the distribution of dye molecules within the porous semiconductor (TiO 2 ). In this paper, we formulate a continuum model for the interaction between the dye molecule Tris(2,2 ′ -bipyridyl)ruthenium(II) (Ru(bpy) 3 2 + ) and titanium dioxide (TiO 2 ) semiconductor. We obtain the equilibrium position at the minimum energy position between the dye molecules and between the dye and TiO 2 nanoporous structure. Our main outcome is an analytical expression for the energy of the two molecules as a function of their sizes. We also show that the interaction energy obtained using the continuum model is in close agreement with molecular dynamics simulations.
Role of a Brief Intensive Observation Area with a Dedicated Team of Doctors in the Management of Acute Heart Failure Patients: A Retrospective Observational Study
Authors: Savioli Gabriele , Ceresa Iride Francesca , Manzoni Federica , Ricevuti Giovanni , Bressan Maria Antonietta , Oddone Enrico
Published: May 2020

Background and objectives: Acute heart failure (AHF) is one of the main causes of hospitalization in Western countries. Usually, patients cannot be admitted directly to the wards (access block) and stay in the emergency room. Holding units are clinical decision units, or observation units, within the ED that are able to alleviate access block and to contribute to a reduction in hospitalization. Observation units have also been shown to play a role in specific clinical conditions, like the acute exacerbation of heart failure. This study aimed to analyze the impact of a brief intensive observation (OBI) area on the management of acute heart failure (AHF) patients. The OBI is a holding unit dedicated to the stabilization of unstable patients with a team of dedicated physicians. Materials and Methods: We conducted a retrospective and single-centered observational study with retrospective collection of the data of all patients who presented to our emergency department with AHF during 2017. We evaluated and compared two cohorts of patients, those treated in the OBI and those who were not, in terms of the reduction in color codes at discharge, mortality rate within the emergency room (ER), hospitalization rate, rate of transfer to less intensive facilities, and readmission rate at 7, 14, and 30 days after discharge. Results: We enrolled 920 patients from 1st January to 31st December. Of these, 61% were transferred to the OBI for stabilization. No statistically significant difference between the OBI and non-OBI populations in terms of age and gender was observed. OBI patients had worse clinical conditions on arrival. The patients treated in the OBI had longer process times, which would be expected, to allow patient stabilization. The stabilization rate in the OBI was higher, since presumably OBI admission protected patients from “worse condition” at discharge. Conclusions: Data from our study show that a dedicated area of the ER, such as the OBI, has progressively allowed a change in the treatment path of the patient, where the aim is no longer to admit the patient for processing but to treat the patient first and then, if necessary, admit or refer. This has resulted in very good feedback on patient stabilization and has resulted in a better management of beds, reduced admission rates, and reduced use of high intensity care beds.
Investigation of Melt Pool Geometry Control in Additive Manufacturing Using Hybrid Modeling
Authors: Mondal Sudeepta , Gwynn Daniel , Ray Asok , Basak Amrita
Published: May 2020

Metal additive manufacturing (AM) works on the principle of consolidating feedstock material in layers towards the fabrication of complex objects through localized melting and resolidification using high-power energy sources. Powder bed fusion and directed energy deposition are two widespread metal AM processes that are currently in use. During layer-by-layer fabrication, as the components continue to gain thermal energy, the melt pool geometry undergoes substantial changes if the process parameters are not appropriately adjusted on-the-fly. Although control of melt pool geometry via feedback or feedforward methods is a possibility, the time needed for changes in process parameters to translate into adjustments in melt pool geometry is of critical concern. A second option is to implement multi-physics simulation models that can provide estimates of temporal process parameter evolution. However, such models are computationally near intractable when they are coupled with an optimization framework for finding process parameters that can retain the desired melt pool geometry as a function of time. To address these challenges, a hybrid framework involving machine learning-assisted process modeling and optimization for controlling the melt pool geometry during the build process is developed and validated using experimental observations. A widely used 3D analytical model capable of predicting the thermal distribution in a moving melt pool is implemented and, thereafter, a nonparametric Bayesian, namely, Gaussian Process (GP), model is used for the prediction of time-dependent melt pool geometry (e.g., dimensions) at different values of the process parameters with excellent accuracy along with uncertainty quantification at the prediction points. Finally, a surrogate-assisted statistical learning and optimization architecture involving GP-based modeling and Bayesian Optimization (BO) is employed for predicting the optimal set of process parameters as the scan progresses to keep the melt pool dimensions at desired values. The results demonstrate that a model-based optimization can be significantly accelerated using tools of machine learning in a data-driven setting and reliable a priori estimates of process parameter evolution can be generated to obtain desired melt pool dimensions for the entire build process.
Development of a Multi-Material Stereolithography 3D Printing Device
Authors: Khatri Bilal , Frey Marco , Raouf-Fahmy Ahmed , Scharla Marc-Vincent , Hanemann Thomas
Published: May 2020

Additive manufacturing, or nowadays more popularly entitled as 3D printing, enables a fast realization of polymer, metal, ceramic or composite devices, which often cannot be fabricated with conventional methods. One critical issue for a continuation of this success story is the generation of multi-material devices. Whilst in fused filament fabrication or 3D InkJet printing, commercial solutions have been realized, in stereolithography only very few attempts have been seen. In this work, a comprehensive approach, covering the construction, material development, software control and multi-material printing is presented for the fabrication of structural details in the micrometer range. The work concludes with a critical evaluation and possible improvements.
Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria
Authors: Navarro Armando , Licona-Moreno Delia , Monsalvo-Reyes Alejandro , Hernández-Chiñas Ulises , Eslava-Campos Carlos A.
Published: May 2020

Background: Escherichia coli and Salmonella are etiologic agents of intestinal infections. A previous study showed the presence of shared epitopes between lipopolysaccharides (LPSs) of E. coli O157 and Salmonella. Aim: Using phage display, the aim of this study is to identify mimotopes of shared epitopes in different enterobacterial LPSs. Methods: We use anti-LPS IgG from E. coli O157 and Salmonella to select peptide mimotopes of the M13 phage. The amino acid sequence of the mimotopes is used to synthesize peptides, which are in turn used to immunize rabbits. The antibody response of the resulting sera against the LPSs and synthetic peptides (SPs) is analyzed by ELISA and by Western blot assays, indicating that LPS sites are recognized by the same antibody. In a complementary test, the reactions of human serum samples obtained from the general population against the SPs and LPSs are also analyzed. Results: From the last biopanning phase, sixty phagotopes are selected. The analysis of the peptide mimotope amino acid sequences shows that in 4 of them the S/N/A/PF motif is a common sequence. Antibodies from the sera of immunized rabbits with SP287/3, SP459/1, SP308/3, and SP073/14 react against both their own peptide and the different LPSs. The Western blot test shows a sera reaction against both the lateral chains and the cores of the LPSs. The analysis of the human sera shows a response against the SPs and LPSs. Conclusion: The designed synthetic peptides are mimotopes of LPS epitopes of Salmonella and E. coli that possess immunogenic capacity. These mimotopes could be considered for use in the design of vaccines against both enterobacteria.
Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate
Authors: Tamires Moreira Melo Nadielle , Pontes Gabriela Coimbra , Procópio Dielle Pierotti , de Gois e Cunha Gabriel Caetano , Eliodório Kevy Pontes , Costa Paes Hugo , Basso Thiago Olitta , Parachin Nádia Skorupa
Published: May 2020

Lactic acid is the monomeric unit of polylactide (PLA), a bioplastic widely used in the packaging, automotive, food, and pharmaceutical industries. Previously, the yeast Komagataella phaffii was genetically modified for the production of lactate from glycerol. For this, the bovine L-lactate dehydrogenase- (LDH)-encoding gene was inserted and the gene encoding the pyruvate decarboxylase (PDC) was disrupted, resulting in the GLp strain. This showed a yield of 67% L-lactic acid and 20% arabitol as a by-product in batches with oxygen limitation. Following up on these results, the present work endeavored to perform a detailed study of the metabolism of this yeast, as well as perturbing arabitol synthesis in an attempt to increase lactic acid titers. The GLp strain was cultivated in a glycerol-limited chemostat at different dilution rates, confirming that the production of both lactic acid and arabitol is dependent on the specific growth rate (and consequently on the concentration of the limiting carbon source) as well as on the oxygen level. Moreover, disruption of the gene encoding arabitol dehydrogenase (ArDH) was carried out, resulting in an increase of 20% in lactic acid and a 50% reduction in arabitol. This study clarifies the underlying metabolic reasons for arabitol formation in K. phaffii and points to ways for improving production of lactic acid using K. phaffii as a biocatalyst.
Antifungal Activity of Gentamicin B1 against Systemic Plant Mycoses
Authors: Banfalvi Gaspar
Published: May 2020

Background: Gentamicin is a broad-spectrum aminoglycoside antibiotic produced by Micromonospora purpurea bacteria, effective against Gram-negative bacterial infections. Major fractions of the gentamicin complex (C1, C1a, C2, C2a) possess weak antifungal activity and one of the minor components (A, A1–A4, B, B1, X), gentamicin B1 was found to be a strong antifungal agent. Methods: This work uses in vitro and in vivo dilution methods to compare the antifusarial, antiaspergillic and anticryptococcal effects of gentamicin derivatives and structurally-related congeners. Results: The in vitro antifusarial activity of gentamicin B1 (minimum inhibitory concentration (MIC) 0.4 μg/mL) and structurally-related compounds (MIC 0.8–12.5 μg/mL) suggests that the purpuroseamine ring substituents are responsible for the specific antimycotic effect. The functional groups of the garoseamine and 2-deoxystreptamine rings of gentamicin derivatives are identical in gentamicin compounds and are unlikely to exert a significant antifungal effect. Among soil dermatophytes, Microsporum gypseum was more susceptible to gentamicin B1 (MIC 3.1 µg/mL) than Trichophyton gypseum (MIC 25 µg/mL). The in vitro antifungal effect of gentamicin B1 against plant pathogenic fungi was comparable to primary antifungal agents. Conclusion: Gentamicin is already in medical use. In vitro and preclinical in vivo synergisms of gentamicin B1 with amphotericin B suggest immediate clinical trials starting with subtoxic doses.
Zebrafish-Based Screening Models for the Identification of Anti-Metastatic Drugs
Authors: Nakayama Joji , Makinoshima Hideki
Published: May 2020

Metastasis, a leading contributor to the morbidity of cancer patients, occurs through a multi-step process: invasion, intravasation, extravasation, colonization, and metastatic tumor formation. Each process is not only promoted by cancer cells themselves but is also affected by their microenvironment. Given this complexity, drug discovery for anti-metastatic drugs must consider the interaction between cancer cells and their microenvironments. The zebrafish is a suitable vertebrate animal model for in vivo high-throughput screening studies with physiological relevance to humans. This review covers the zebrafish model used to identify anti-metastatic drugs.
Synthesis, Characterization and Bioassay of Novel Substituted 1-(3-(1,3-Thiazol-2-yl)phenyl)-5-oxopyrrolidines
Authors: Sapijanskaitė-Banevič Birutė , Šovkovaja Božena , Vaickelionienė Rita , Šiugždaitė Jūratė , Mickevičiūtė Eglė
Published: May 2020

Thiazole derivatives attract the attention of scientists both in the field of organic synthesis and bioactivity research due to their high biological activity. In the present study, thiazole ring was obtained by the interaction of 1-(4-(bromoacetyl)phenyl)-5-oxopyrrolidine-3-carboxylic acid with thiocarbamide or benzenecarbothioamide, as well as tioureido acid. A series of substituted 1-(3-(1,3-thiazol-2-yl)phenyl)-5-oxopyrrolidines with pyrrolidinone, thiazole, pyrrole, 1,2,4-triazole, oxadiazole and benzimidazole heterocyclic fragments were synthesized and their antibacterial properties were evaluated against Gram-positive strains of Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes and Gram-negative Pseudomonas aeruginosa, Escherichia coli and Salmonella enterica enteritidis. The vast majority of compounds exhibited between twofold and 16-fold increased antibacterial effect against the test-cultures when compared with Oxytetracycline.
Gender Preference and Difference in Behavior Modeling in Fitness Applications: A Mixed-Method Approach
Authors: Oyibo Kiemute , Vassileva Julita
Published: May 2020

In recent years, the employment of behavior models to motivate behavior change has become a global trend in fitness application design. However, there is hardly any large-scale study of these applications to understand users’ exercise-type preferences, their drivers and barriers, and the potential of employing them for gender-based tailoring. To bridge this gap, we conducted a mixed-method study among 669 participants to investigate users’ exercise-type preferences (their drivers and barriers) and how they and gender can impact users’ social-cognitive beliefs and projected performance of bodyweight exercises. Firstly, we presented to the study participants a behavior model performing push-up or squat bodyweight exercise in a fitness application and asked them to rate their perceived self-efficacy, self-regulation, outcome expectation, and projected (exercise) performance level as observers of the behavior model. Secondly, we presented the study participants with a preselected list of commonly employed exercise types in fitness applications and requested them to identify their most/least preferred, and the reasons behind their choices. Our results showed that there were differences between both genders in their exercise-type preferences, perceived self-efficacy and projected exercise performance level. Males prefer push-up, squat, crunch, plank, and chair dip the most, with effectiveness being the most important driver, followed by ease of performance and improvement of the physique, look, and appearance. On the other hand, females prefer squat, crunch, jumping jack, step up, and plank the most, with ease of performance being the most important driver, followed by improvement of the physique, look, appearance, and effectiveness. Moreover, males prefer running in place the least, while females prefer push-up the least, with perceived difficulty being the greatest barrier for both genders. Moreover, our analysis of variance supported the female’s least preference for a push-up. Females have a lower perceived self-efficacy and projected performance level for push-up than males. We discussed the implications of our findings and provided guidelines for tailoring fitness applications on the market to users’ preferences and gender.
Phytosterols Supplementation Reduces Endothelin-1 Plasma Concentration in Moderately Hypercholesterolemic Individuals Independently of Their Cholesterol-Lowering Properties
Authors: Oliveira Godoy Ilha Angela , Sutti Nunes Valeria , Silva Afonso Milessa , Regina Nakandakare Edna , da Silva Ferreira Guilherme , de Paula Assis Bombo Renata , Rodrigues Giorgi Ricardo , Marcondes Machado Roberta , Carlos Rocha Quintão Eder , Lottenberg Ana Maria
Published: May 2020

Experimental and clinical studies have demonstrated the effect of phytosterols (PS) on reducing plasma levels of cholesterol and LDL-c, but the effects of plant sterols beyond cholesterol-lowering are still questionable. Since inflammation and endothelial dysfunction are involved in the pathogenesis of atherosclerosis, this study aims to evaluate the effect of PS on biomarkers involved in atherosclerosis progression and whether these effects are independent of alterations in plasma LDL-c levels. Thirty-eight moderately hypercholesterolemic volunteers (58 ± 12 years; LDL-c ≥ 130 mg/dL) were randomly assigned to consume 400 mL/day of soy milk or soy milk + PS (1.6 g/day) for 4 weeks in a double-blind, placebo-controlled, cross-over study. Blood samples were collected and lipid profiles and biomarkers for inflammation and endothelial dysfunction determined. The results showed that PS treatment reduced endothelin-1 plasma concentration by 11% (p = 0.02) independently of variations in plasma levels of LDL-c. No alterations were observed regarding fibrinogen, IL-6, hs-CRP, SAA, TNFα, or VCAM-1 between placebo and PS-treated groups. Furthermore, PS reduced total plasma cholesterol concentration (−5,5%, p < 0.001), LDL-c (−6.4%, p < 0.05), triglycerides (−8.3%, p < 0.05), and apo B (−5.3%, p < 0.05), without changing HDL-c concentration (p > 0.05). Therefore, PS supplementation effectively lowers endothelin-1 independently of the reductions in plasma levels of LDL-c, contributing to the comprehension of the effect of plant sterols on endothelial function and prevention of cardiovascular diseases.
Changes in Bone Marrow Fat upon Dietary-Induced Weight Loss
Authors: Spurny Manuela , Jiang Yixin , Sowah Solomon A. , Schübel Ruth , Nonnenmacher Tobias , Bertheau Robert , Kirsten Romy , Johnson Theron , Hillengass Jens , Schlett Christopher L. , von Stackelberg Oyunbileg , Ulrich Cornelia M. , Kaaks Rudolf , Kauczor Hans-Ulrich , Kühn Tilman , Nattenmüller Johanna
Published: May 2020

Background: Bone marrow fat is implicated in metabolism, bone health and haematological diseases. Thus, this study aims to analyse the impact of moderate weight loss on bone marrow fat content (BMFC) in obese, healthy individuals. Methods: Data of the HELENA-Trial (Healthy nutrition and energy restriction as cancer prevention strategies: a randomized controlled intervention trial), a randomized controlled trial (RCT) among 137 non-smoking, overweight or obese participants, were analysed to quantify the Magnetic Resonance Imaging (MRI)-derived BMFC at baseline, after a 12-week dietary intervention phase, and after a 50-week follow-up. The study cohort was classified into quartiles based on changes in body weight between baseline and week 12. Changes in BMFC in respect of weight loss were analysed by linear mixed models. Spearman’s coefficients were used to assess correlations between anthropometric parameters, blood biochemical markers, blood cells and BMFC. Results: Relative changes in BMFC from baseline to week 12 were 0.0 ± 0.2%, −3.2 ± 0.1%, −6.1 ± 0.2% and −11.5 ± 0.6% for Q1 to Q4. Across all four quartiles and for the two-group comparison, Q1 versus Q4, there was a significant difference (p < 0.05) for changes in BMFC. BMFC was not associated with blood cell counts and showed only weaker correlations (<0.3) with metabolic biomarkers. Conclusion: Weight loss is associated with a decrease of BMFC. However, BMFC showed no stronger associations with inflammatory and metabolic biomarkers.
Validation of the Telephone-Administered Version of the Mediterranean Diet Adherence Screener (MEDAS) Questionnaire
Authors: Gregório Maria João , Rodrigues Ana M. , Salvador Clara , Dias Sara S. , de Sousa Rute D. , Mendes Jorge M. , Coelho Pedro S. , Branco Jaime C. , Lopes Carla , Martínez-González Miguel A. , Graça Pedro , Canhão Helena
Published: May 2020

A 14-Item Mediterranean Diet Adherence Screener (MEDAS) questionnaire was developed and validated in face-to-face interviews, but not via telephone. The aims of this study were to evaluate the validity and reliability of a telephone-administered version of the MEDAS as well as to validate the Portuguese version of the MEDAS questionnaire. A convenience community-based sample of adults (n = 224) participated in a three-stage survey. First, trained researchers administered MEDAS via a telephone. Second, the Portuguese version of Food Frequency Questionnaire (FFQ), and MEDAS were administered in a semi-structured face-to-face interview. Finally, MEDAS was again administered via telephone. The telephone-administered MEDAS questionnaire was compared with the face-to-face-version using several metrics. The telephone-administered MEDAS was significantly correlated with the face-to-face-administered MEDAS [r = 0.805, p < 0.001; interclass correlation coefficient (ICC) = 0.803, p < 0.001] and showed strong agreement (k = 0.60). The MEDAS scores that were obtained in the first and second telephone interviews were significantly correlated (r = 0.661, p < 0.001; ICC = 0.639, p < 0.001). The overall agreement between the Portuguese version of MEDAS and the FFQ-derived Mediterranean diet adherence score had a Cohen’s k = 0.39. The telephone-administered version of MEDAS is a valid tool for assessing the adherence to the Mediterranean diet and acquiring data for large population-based studies.
Effects of a High-Protein Diet on Cardiometabolic Health, Vascular Function, and Endocannabinoids—A PREVIEW Study
Authors: Tischmann Lea , Drummen Mathijs , Joris Peter , Gatta-Cherifi Blandine , Raben Anne , Fogelholm Mikael , Matias Isabelle , Cota Daniela , Mensink Ronald , Westerterp-Plantenga Margriet , Adam Tanja
Published: May 2020

An unfavorable lipid profile and being overweight are known mediators in the development of cardiovascular disease (CVD) risk. The effect of diet, particularly high in protein, remains under discussion. Therefore, this study examines the effects of a high-protein (HP) diet on cardiometabolic health and vascular function (i.e., endothelial function, arterial stiffness, and retinal microvascular structure), and the possible association with plasma endocannabinoids and endocannabinoid-related compounds in overweight participants. Thirty-eight participants (64.5 ± 5.9 (mean ± SD) years; body mass index (BMI) 28.9 ± 4.0 kg/m2) were measured for 48 h in a respiration chamber after body-weight maintenance for approximately 34 months following weight reduction. Diets with either a HP (n = 20) or moderate protein (MP; n = 18) content (25%/45%/30% vs. 15%/55%/30% protein/carbohydrate/fat) were provided in energy balance. Validated markers for cardiometabolic health (i.e., office blood pressure (BP) and serum lipoprotein concentrations) and vascular function (i.e., brachial artery flow-mediated vasodilation, pulse wave analysis and velocity, and retinal microvascular calibers) were measured before and after those 48 h. Additionally, 24 h ambulatory BP, plasma anandamide (AEA), 2-arachidonoylglycerol (2-AG), oleoylethanolamide (OEA), palmitoylethanolamide (PEA), and pregnenolone (PREG) were analyzed throughout the day. Office and ambulatory BP, serum lipoprotein concentrations, and vascular function markers were not different between the groups. Only heart rate (HR) was higher in the HP group. HR was positively associated with OEA, while OEA and PEA were also positively associated with total cholesterol (TC) and low-density lipoprotein (LDL) cholesterol concentrations. Vascular function markers were not associated with endocannabinoids (or endocannabinoid-related substances). In conclusion, the HP diet did not affect cardiometabolic health and vascular function in overweight participants after completing a weight-loss intervention. Furthermore, our data indicate a possible association between OEA and PEA with TC and LDL cholesterol.
Diet and Nutrition Status of Mongolian Adults
Authors: Bromage Sabri , Daria Tselmen , Lander Rebecca L. , Tsolmon Soninkhishig , Houghton Lisa A. , Tserennadmid Enkhjargal , Gombo Nyamjargal , Gibson Rosalind S. , Ganmaa Davaasambuu
Published: May 2020

(1) Background: Aspects of the Mongolian food supply, including high availability of animal-source foods and few plant foods, are plausibly associated with disease in the population. Data on Mongolian diets are lacking, and these risks are poorly quantified. The purpose of this study was to provide a multifaceted nutritional analysis of the modern Mongolian diet. (2) Methods: The study population consisted of 167 male and 167 female healthy non-pregnant urban and nomadic adults (22–55 years) randomly selected from lists of residents in 8 regions. From 2011–2016, 3-day weighed diet records and serum were collected twice from each participant in summer and winter; anthropometry was collected once from each participant. Serum was analyzed for biomarkers, and nutrient intake computed using purpose-built food composition data and adjusted for within-person variation. Exploratory dietary patterns were derived and analyzed for associations with diet and nutrition measurements. (3) Results: We collected 1838 of an expected 1986 diet records (92.5%), 610/658 serum samples (92.7%), and 315/334 height and weight measurements (94.3%). Sixty-one percent of men and 51% of women were overweight or obese. Consumption of red meat, refined grains, and whole-fat dairy was high, while that of fruits, non-tuberous vegetables, eggs, nuts and seeds, fish and poultry, and whole grains was low. Dairy and red meat were more consumed in summer and winter, respectively. Dietary inadequacy of 10 of 21 assessed nutrients, including fiber, folate, and vitamin D were >50% prevalent, while protein, zinc, and vitamin B12 inadequacy were low. Biochemical evidence of iron and vitamin A deficiency was also low. Three dietary patterns (Urban, Transitional, Nomadic) explained 41% of variation in food consumption. The Urban pattern was positively associated with BMI in multivariate analysis. (4) Conclusions: Results indicate a high prevalence of key dietary inadequacies and overweight among Mongolian adults. Prior studies by our group have suggested that expanded supplementation and food fortification would be effective in addressing micronutrient inadequacies; these strategies should be coupled with measures to mitigate the growing burden of chronic disease.
The Relationship between Estrogen-Related Signaling and Human Papillomavirus Positive Cancers
Authors: James Claire D. , Morgan Iain M. , Bristol Molly L.
Published: May 2020

High risk-human papillomaviruses (HPVs) are known carcinogens. Numerous reports have linked the steroid hormone estrogen, and the expression of estrogen receptors (ERs), to HPV-related cancers, although the exact nature of the interactions remains to be fully elucidated. Here we will focus on estrogen signaling and describe both pro and potentially anti-cancer effects of this hormone in HPV-positive cancers. This review will summarize: (1) cell culture-related evidence, (2) animal model evidence, and (3) clinical evidence demonstrating an interaction between estrogen and HPV-positive cancers. This comprehensive review provides insights into the potential relationship between estrogen and HPV. We suggest that estrogen may provide a potential therapeutic for HPV-related cancers, however additional studies are necessary.
A Review: Wolbachia-Based Population Replacement for Mosquito Control Shares Common Points with Genetically Modified Control Approaches
Authors: Yen Pei-Shi , Failloux Anna-Bella
Published: May 2020

The growing expansion of mosquito vectors has made mosquito-borne arboviral diseases a global threat to public health, and the lack of licensed vaccines and treatments highlight the urgent need for efficient mosquito vector control. Compared to genetically modified control strategies, the intracellular bacterium Wolbachia, endowing a pathogen-blocking phenotype, is considered an environmentally friendly strategy to replace the target population for controlling arboviral diseases. However, the incomplete knowledge regarding the pathogen-blocking mechanism weakens the reliability of a Wolbachia-based population replacement strategy. Wolbachia infections are also vulnerable to environmental factors, temperature, and host diet, affecting their densities in mosquitoes and thus the virus-blocking phenotype. Here, we review the properties of the Wolbachia strategy as an approach to control mosquito populations in comparison with genetically modified control methods. Both strategies tend to limit arbovirus infections but increase the risk of selecting arbovirus escape mutants, rendering these strategies less reliable.
Characterization of Colletotrichum ocimi Population Associated with Black Spot of Sweet Basil (Ocimum basilicum) in Northern Italy
Authors: Cacciola Santa Olga , Gilardi Giovanna , Faedda Roberto , Schena Leonardo , Pane Antonella , Garibaldi Angelo , Gullino Maria Lodovica
Published: May 2020

Black spot is a major foliar disease of sweet basil (Ocimum basilicum) present in a typical cultivation area of northern Italy, including the Liguria and southern Piedmont regions, where this aromatic herb is an economically important crop. In this study, 15 Colletotrichum isolates obtained from sweet basil plants with symptoms of black spot sampled in this area were characterized morphologically and by nuclear DNA analysis using internal transcribed spacers (ITS) and intervening 5.8S nrDNA as well as part of the β-tubulin gene (TUB2) regions as barcode markers. Analysis revealed all but one isolate belonged to the recently described species C. ocimi of the C. destructivum species complex. Only one isolate was identified as C. destructivum sensu stricto (s.s.). In pathogenicity tests on sweet basil, both C. ocimi and C. destructivum s.s. isolates incited typical symptoms of black spot, showing that although C. ocimi prevails in this basil production area, it is not the sole causal agent of black spot in northern Italy. While no other hosts of C. ocimi are known worldwide, the close related species C. destructivum has a broad host range, suggesting a speciation process of C. ocimi within this species complex driven by adaptation to the host.
Dental Composition Modified with Aryloxyphosphazene Containing Carboxyl Groups
Authors: Chistyakov Evgeniy M. , Kolpinskaya Natalya , Posokhova Vera , Chuev Vladimir
Published: May 2020

A modifier consisting of the mixture of cyclotriphosphazenes containing 4-allyl-2-methoxyphenoxy and β-carboxyethenylphenoxy moieties was developed for administration with acrylate dental restorative compositions. The synthesized compounds were characterized by 1H and 13C NMR spectroscopy and MALDI-TOF mass spectrometry. The optimal conditions to combine the modifier with the starting dental mixture consisting of bis-GMA and TGM-3 were revealed by differential scanning calorimetry (DSC) method. Properties of the cured modified compositions were evaluated for the compliance with requirements of ISO 4049:2019. It was found that these compositions possess the increased adhesion to dental tissues and cure depth and the decreased water sorption and water solubility. The values of elastic modules, destructive compressive stress and microhardness were also increasing along with the increased content of the modifier in the composition.