bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of a prognostic evaluator from glutamine metabolic heterogeneity studies within and between tissues in hepatocellular carcinoma
Authors: Bao Jie , Yu Yan
Published: October 2023

Background: The liver is the major metabolic organ of the human body, and abnormal metabolism is the main factor influencing hepatocellular carcinoma (HCC). This study was designed to determine the effect of glutamine metabolism on HCC heterogeneity and to develop a prognostic evaluator based on the heterogeneity study of glutamine metabolism within HCC tumors and between tissues.

Methods: Single-cell transcriptome data were extracted from the GSE149614 dataset and processed using the Seurat package in R for quality control of these data. HCC subtypes in the Cancer Genome Atlas and the GSE14520 dataset were identified via consensus clustering based on glutamine family amino acid metabolism (GFAAM) process genes. The machine learning algorithms gradient boosting machine, support vector machine, random forest, eXtreme gradient boosting, decision trees, and least absolute shrinkage and selection operator were utilized to develop the prognosis model of differentially expressed genes among the molecular gene subtypes.

Results: The samples in the GSE149614 dataset included 10 cell types, and there was no significant difference in the GFAAM pathway. HCC was classified into three molecular subtypes according to GFAAM process genes, showing molecular heterogeneity in prognosis, clinicopathological features, and immune cell infiltration. C1 showed the worst survival rate and the highest immune score and immune cell infiltration. A six-gene model for prognostic and immunotherapy responses was constructed among subtypes, and the calculated high-risk score was significantly correlated with poor prognosis, high immune abundance, and a low response rate of immunotherapy in HCC.

Conclusion: Our discovery of GFAAM-associated marker genes may help to further decipher the role in HCC occurrence and progression. In particular, this six-gene prognostic model may serve as a predictor of treatment and prognosis in HCC patients.
Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection
Authors: Wang Huiqiang , Chen Fenbei , Wang Shicong , Li Yuhuan , Liu Ting , Li Yinghong , Deng Hongbin , Dong Jingwen , Pang Jing , Song Danqing , Zhang Dousheng , Yu Juan , Wang Yanxiang
Published: October 2023

Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) infection, currently lacks specific preventive and therapeutic interventions. Here, we demonstrated that Pien Tze Huang (PZH) could dose-dependently inhibit EV-A71 replication at the cellular level, resulting in significant reductions in EV-A71 virus protein 1 (VP1) expression and viral yields in Vero and human rhabdomyosarcoma cells. More importantly, we confirmed that PZH could protect mice from EV-A71 infection for the first time, with Ribavirin serving as a positive control. PZH treatment reduced EV-A71 VP1 protein expression, viral yields in infected muscles, and improved muscle pathology. Additionally, we conducted a preliminary mechanism study using quantitative proteomics. The results suggested that the suppression of the PI3K/AKT/mTOR and NF-κB signaling pathways may contribute to the anti-EV-A71 activity of PZH. These findings provide strong evidence supporting the potential therapeutic application of PZH for EV-A71 infection management.
Efficacy of extracellular vesicles as a cell-free therapy in colitis: a systematic review and meta-analysis of animal studies
Authors: Hou Jun-Jie , Li Wei-Wei , Wang Xiao-Li , Ma A-Huo , Qin Yue-Hua
Published: October 2023

Background: Extracellular vesicles (EVs) mediate inflammation, immune responses, gut barrier integrity, and intestinal homeostasis. Recently, the application of EVs in the treatment of inflammatory bowel disease (IBD) has been under intensive focus. Some studies have been conducted in animal models of colitis, while systematic reviews and meta-analyses are lacking. The current study aimed to conduct a systematic review and meta-analysis of studies investigating the efficacy of EVs on IBD.

Methods: A systematic retrieval of all studies in PubMed, EMBASE, MEDLINE, Web of Science, and Cochrane Library reported the effects of EVs in the colitis model up to 22 June 2023. The methodological quality was assessed based on SYRCLE’s risk of bias (RoB) tool. Disease activity index (DAI), myeloperoxidase activity (MPO), histopathological score (HS), and inflammatory cytokines (TNF-α, NF-κB, IL-1β, IL-6, and IL-10) were extracted as analysis indicators by Web Plot Digitizer 4.5. A meta-analysis was performed to calculate the standardized mean difference and 95% confidence interval using random-effect models by Review Manager 5.3 and STATA 14.0 software.

Results: A total of 21 studies were included in this meta-analysis. Although the heterogeneity between studies and the potential publication bias limits confidence in the extent of the benefit, EV treatment was superior to the control in the colitis evaluation with reduced DAI, HS, MPO activity, and pro-inflammatory cytokines, including TNF-α, NF-κB, IL-1β, and IL-6, while increasing the content of anti-inflammatory cytokine IL-10 (all p < 0.05).

Conclusion: Our meta-analysis results supported the protective effect of EVs on colitis rodent models based on their potential role in IBD therapy and propelling the field toward clinical studies.
Lonicerae Japonicae Caulis: a review of its research progress of active metabolites and pharmacological effects
Authors: Cao Yu-Xia , Ji Peng , Wu Fan-Lin , Dong Jia-Qi , Li Chen-Chen , Ma Ting , Yang Hao-Chi , Wei Yan-Ming , Hua Yong-Li
Published: October 2023

Lonicerae Japonicae Caulis is the aboveground stem part of the Lonicera Japonica Thunb, which belongs to the medicine food homology species in China. It has the effects of clearing away heat, toxic material, dredging wind and unblocking collaterals. Modern research shows that it contains various active metabolites and a wide range of pharmacological effects, which is of great research and clinical application value. It mainly contains organic acids, volatile oils, flavonoids, triterpenes, triterpene saponins and other active metabolites. Its pharmacological effects mainly include anti-inflammatory, antibacterial, antitumor, antioxidant, and repairing bone and soft tissue. Based on the literature reports in recent years, the active metabolites, pharmacological effects and mechanisms of Lonicerae Japonicae Caulis were sorted out and summarized. It lays a foundation for explaining the efficacy material basis and application value of Lonicerae Japonicae Caulis. It aims to provide a reference for the in-depth research, development and utilization of Lonicerae Japonicae Caulis.
Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage
Authors: Liao Zhi , Liu Fei , Wang Ying , Fan Xiaojun , Li Yingao , He Jianyu , Buttino Isabella , Yan Xiaojun , Zhang Xiaolin , Shi Ge
Published: October 2023

Mytilus coruscus is an economically important marine calcifier living in the Yangtze River estuary sea area, where seasonal fluctuations in natural pH occur owing to freshwater input, resulting in a rapid reduction in seawater pH. In addition, Mytilus constantly suffers from shell fracture or injury in the natural environment, and the shell repair mechanisms in mussels have evolved to counteract shell injury. Therefore, we utilized shell-complete and shell-damaged Mytilus coruscus in this study and performed transcriptomic analysis of the mantle to investigate whether the expression of mantle-specific genes can be induced by acute seawater acidification and how the mantle responds to acute acidification during the shell repair process. We found that acute acidification induced more differentially expressed genes than shell damage in the mantle, and the biomineralization-related Gene Ontology terms and KEGG pathways were significantly enriched by these DEGs. Most DEGs were upregulated in enriched pathways, indicating the activation of biomineralization-related processes in the mussel mantle under acute acidification. The expression levels of some shell matrix proteins and antimicrobial peptides increased under acute acidification and/or shell damage, suggesting the molecular modulation of the mantle for the preparation and activation of the shell repairing and anti-infection under adverse environmental conditions. In addition, morphological and microstructural analyses were performed for the mantle edge and shell cross-section, and changes in the mantle secretory capacity and shell inner film system induced by the two stressors were observed. Our findings highlight the adaptation of M. coruscus in estuarine areas with dramatic fluctuations in pH and may prove instrumental in its ability to survive ocean acidification.
Twenty-four years lucerne (Medicago sativa L.) breeder seed production in India: a retrospective study
Authors: Chand Subhash , Roy Ajoy Kumar , Singh Tejveer , Agrawal Rajiv Kumar , Yadav Vijay Kumar , Kumar Sanjay , Malaviya Devendra Ram , Chandra Amaresh , Yadava Devendra Kumar
Published: October 2023

Lucerne (Medicago sativa L.) is the second most significant winter leguminous fodder crop after berseem in India. Breeder seed (BS) is the first stage of the seed production chain, as it is the base material for producing foundation and certified seeds. In India, lucerne BS demand has been reduced by 85.58% during the last 24 years (1998–1999 to 2021–2022), declining from 2150 kg to 310 kg. Out of 14 varieties released and notified so far, only nine varieties entered the seed chain since 1998–1999. It shows narrow varietal diversification and, hence, needs robust breeding programs towards enriching genetic variability and varietal development. The present study also highlights the disparity in BS demand and production over the years and puts forth the possible reasons behind the reduction in BS demand and production in the country. Out of the nine varieties, the BS demand of Anand-2 (53.11%) was highest, followed by Type-9 (19.44%) and RL-88 (13.60%). Varietal replacement rate (VRR) was found to be moderate, i.e., 23.67% for the varieties having <5 years old age in the last 3 years (2019–2020 to 2021–2022). It has also been estimated that BS produced (233 kg) during 2021–2022 can cover the approximate area of 6,300 ha at farmers’ fields in 2024–2025 if the seed chain functions 100%, effectively. The present study provides a holistic overview of lucerne BS demand and production, challenges in BS production, and the way forward to develop more varieties and surplus BS production in the country.
Various tomato cultivars display contrasting morphological and molecular responses to a chronic heat stress
Authors: Bollier N. , Micol-Ponce R. , Dakdaki A. , Maza E. , Zouine M. , Djari A. , Bouzayen M. , Chevalier C. , Delmas F. , Gonzalez N. , Hernould M.
Published: October 2023

Climate change is one of the biggest threats that human society currently needs to face. Heat waves associated with global warming negatively affect plant growth and development and will increase in intensity and frequency in the coming years. Tomato is one of the most produced and consumed fruit in the world but remarkable yield losses occur every year due to the sensitivity of many cultivars to heat stress (HS). New insights into how tomato plants are responding to HS will contribute to the development of cultivars with high yields under harsh temperature conditions. In this study, the analysis of microsporogenesis and pollen germination rate of eleven tomato cultivars after exposure to a chronic HS revealed differences between genotypes. Pollen development was either delayed and/or desynchronized by HS depending on the cultivar considered. In addition, except for two, pollen germination was abolished by HS in all cultivars. The transcriptome of floral buds at two developmental stages (tetrad and pollen floral buds) of five cultivars revealed common and specific molecular responses implemented by tomato cultivars to cope with chronic HS. These data provide valuable insights into the diversity of the genetic response of floral buds from different cultivars to HS and may contribute to the development of future climate resilient tomato varieties.
Coexpression and synexpression patterns across languages: comparative concepts and possible explanations
Authors: Haspelmath Martin
Published: October 2023

Meanings and linguistic shapes (or forms) do not always map onto each other in a unique way, and linguists have used all kinds of different terms for such situations: Ambiguity, polysemy, syncretism, lexicalization, semantic maps; portmanteau, cumulative exponence, feature bundling, underspecification, and so on. In the domain of lexical comparison, the termcolexificationhas become generally established in recent years, and in the present paper, I extend this word-formation pattern in a regular way (cogrammification,coexpression;syllexification,syngrammification,synexpression). These novel terms allow us to chart the range of relevant phenomena in a systematic way across the grammar-lexicon continuum, and to ask whether highly general explanations of coexpression and synexpression patterns are possible. While there is no new proposal for explaining coexpression here, I will suggest that frequency of occurrence plays a crucial role in explaining synexpression patterns.
Children’s pre-academic school readiness and home learning activities: a moderated-mediation analysis of home visiting
Authors: Avelar Daniela , Hull Darrell , Middlemiss Wendy
Published: October 2023

Introduction

The current study explores the relation between parent involvement and children’s school readiness for 568 families enrolled in the Home Instruction of Preschool Youngsters (HIPPY) program in Texas. Parent involvement in children’s learning is a focus of the HIPPY curriculum.

Methods

In this analysis, conditional process models were run to examine the relations between children’s school readiness and engagement in home learning activities, parents’ education level, program language (English or Spanish), both before and after completing a year of the program.

Results

At pretest but not posttest, program language moderated the direct relation between parents’ education level and children’s school readiness and parents with high levels of education were more likely to engage their children in educational activities. Engagement in home learning activities was associated with school readiness regardless of parents’ education level and language.

Discussion

These findings provide a better understanding of the variables associated with school readiness for HIPPY families, indicating the importance of home learning activities—a variable that may be more amenable to change within intervention programs. Thus, focusing on home learning activities may contribute to decreasing discrepancies in children’s preparedness for school entry that are generally identified across language and parent education. Findings may have practical implications for other home visitor programs working with similar populations.
Are we really studying resilience in sport? A critical review of adopted methodologies
Authors: Kegelaers Jolan
Published: October 2023

Psychological resilience has gained considerable attention in sport. Nevertheless, the construct often remains poorly understood and multiple conceptual and methodological issues pervade the literature. The purpose of the present article is to provide a critical review of the commonly adopted methodologies to study resilience in sport. This review is divided into four sections. The first section will briefly discuss opposing conceptualizations of resilience as a static trait or a dynamic process. The second section will then discuss key methodological implications relating to the conceptualization of resilience as a dynamic process. In the third section, common methodologies to study resilience in sport are presented and critically reviewed. These methodologies are broadly divided into: (i) self-report resilience measures, (ii) qualitative research, and (iii) direct assessment of functioning in relation to observed adversity. In the final section, some avenues for future research are offered.
Top management team career experience heterogeneity, digital transformation, and the corporate green innovation: a moderated mediation analysis
Authors: Gao Daquan , Li Songsong , Guo Chang
Published: October 2023

Introduction

Drawing upon upper echelon theory and the resource-based view, this study employs a moderated mediation model to investigate the moderating role and underlying mechanisms of digital transformation in the influence of top management teams (TMT) on corporate green innovation.

Methods

Our analysis of panel data from 19,155 Chinese A-share listed companies (2011–2020) demonstrates that TMT career experience heterogeneity has a positive effect on green innovation, a relationship that is further strengthened by digital transformation.

Results

This study shows the role of digital transformation in amplifying the effects of TMT diversity on green innovation and the crucial role of industry-academia-research collaboration as a mediator. Heterogeneity analysis highlights that non-state-owned enterprises (non-SOEs) show more agility than state-owned enterprises (SOEs) in leveraging heterogeneous TMT to drive green innovation. Conversely, green innovation in SOEs benefits more from digital transformation, which includes both its direct and indirect effects of digital transformation. Enterprises located in non-Yangtze River Economic Belt regions benefit more from digital transformation, demonstrating the importance of a balanced distribution of digital resources.

Discussion

This study provides novel insights into leveraging inclusive leadership and digital capabilities to enhance ecological sustainability. This study underscores the potential of diversified TMTs and digitalization technology integration to catalyze green innovation, which is critical for environmentally responsible transformation.
Trends in prescription therapy for adolescents with depression in nine major areas of China during 2017–2021
Authors: Wang Li , Zhou Linpo , Zhu Yao , Yan Jingjing , Bu Na , Fei Weidong , Wu Fan
Published: October 2023

Objective

To date, no national-scale drug usage survey for adolescents with depression has been conducted in China, and the purpose of this study was to examine the national trends in prescriptions in Chinese adolescent depression patients from 2017 to 2021.

Methods

Prescribing data were extracted from the Hospital Prescription Analysis Cooperative Project. The average number of patients per year, the cost of treatment, and the prescription patterns (monotherapy vs. combination therapy) were analyzed, and subgroup analyses were conducted depending on age, sex, and drug class.

Results

The study included 674,099 patients from 136 hospitals located in nine major areas of China. Of all patients, the proportion of adolescents increased from 1.63% in 2017 to 6.75% in 2021. Visits by adolescent depression patients increased from 1,973 in 2017 to 9,751 in 2021, and the corresponding cost increased from 607,598 Chinese Yuan in 2017 to 2,228,884 Chinese Yuan in 2021. The incidence of adolescent depression among female individuals was far beyond that among male individuals. Combination therapy was more frequent than monotherapy, and the most commonly prescribed drugs were antidepressants, antipsychotics, antiepileptics, and antianxietics. Despite the use of sertraline decreasing from 47.90 to 43.39%, it was the most frequently used drug.

Conclusion

In summary, the prescriptions and cost of treatment for adolescent depression patients both increased rapidly. The widespread use of those drugs with weak clinical evidence reflects the current state of China, which should arouse our attention. The study can provide references for clinical treatment decisions and a basis for more efficient allocation of healthcare resources by the government.
Understanding cross-data dynamics of individual and social/environmental factors through a public health lens: explainable machine learning approaches
Authors: Jeong Siwoo , Yun Sung Bum , Park Soon Yong , Mun Sungchul
Published: October 2023

Introduction

The rising prevalence of obesity has become a public health concern, requiring efficient and comprehensive prevention strategies.

Methods

This study innovatively investigated the combined influence of individual and social/environmental factors on obesity within the urban landscape of Seoul, by employing advanced machine learning approaches. We collected ‘Community Health Surveys’ and credit card usage data to represent individual factors. In parallel, we utilized ‘Seoul Open Data’ to encapsulate social/environmental factors contributing to obesity. A Random Forest model was used to predict obesity based on individual factors. The model was further subjected to Shapley Additive Explanations (SHAP) algorithms to determine each factor’s relative importance in obesity prediction. For social/environmental factors, we used the Geographically Weighted Least Absolute Shrinkage and Selection Operator (GWLASSO) to calculate the regression coefficients.

Results

The Random Forest model predicted obesity with an accuracy of >90%. The SHAP revealed diverse influential individual obesity-related factors in each Gu district, although ‘self-awareness of obesity’, ‘weight control experience’, and ‘high blood pressure experience’ were among the top five influential factors across all Gu districts. The GWLASSO indicated variations in regression coefficients between social/environmental factors across different districts.

Conclusion

Our findings provide valuable insights for designing targeted obesity prevention programs that integrate different individual and social/environmental factors within the context of urban design, even within the same city. This study enhances the efficient development and application of explainable machine learning in devising urban health strategies. We recommend that each autonomous district consider these differential influential factors in designing their budget plans to tackle obesity effectively.
Case Report: Radiation necrosis mimicking tumor progression in a patient with extranodal natural killer/T-cell lymphoma
Authors: Chen Boxiao , Fan Yili , Wang Luyao , Zhang Jiawei , Xin Dijia , Qiu Xi , Jiang Huawei , Li Baizhou , Chen Qin , Wang Chao , Xiao Xibin , Huang Liansheng , Xu Yang
Published: October 2023

Radiation-induced cerebral necrosis, also known as radiation encephalopathy, is a debilitating condition that significantly impacts the quality of life for affected patients. Secondary central nervous system lymphoma (SCNSL) typically arises from highly aggressive mature B-cell lymphoma, but rarely from extranodal natural killer T-cell lymphoma (ENKTL). Treatment will be guided by differentiation between lymphoma progression from brain necrosis, and is particularly important for critically ill patients in an acute setting. However, differential diagnosis remains challenging because they share similar clinical manifestations and have no specific imaging features. We present the case of a 52-year-old man with ENKTL who suffered an emergency brain herniation secondary to massive radiation necrosis. The diagnosis established by brain biopsy ultimately led to appropriate treatment. The importance of the diagnostic biopsy is highlighted in this case for distinguishing between radiation necrosis and SCNSL.
Comparative analysis of microbiota in the ceca of broiler chickens with necrotic enteritis fed a commercial corn diet or with corn high in flavonoids (PennHFD1)
Authors: Miska Katarzyna B. , Proszkowiec-Weglarz Monika , Buiatte Vinicius , Mahmoud Mahmoud , Lesko Tyler , Jenkins Mark C. , Chopra Surinder , Lorenzoni Alberto Gino
Published: October 2023

Necrotic enteritis (NE) is a disease of the gastrointestinal tract that is common in broiler chickens and causes substantial economic losses to the poultry industry worldwide. The removal of many antimicrobials in poultry diets has driven the search for alternatives. The purpose of this study was to determine the microbiota changes in the cecal luminal (CE-L) and mucosal (CE-M) populations of broiler chickens undergoing clinical NE (co-infected with Eimeria maxima and Clostridium perfringens) while fed a diet containing a flavonoid rich corn (PennHFD1) or control diet using commercial corns. It was previously shown that chickens fed a diet high in flavonoids had improved performance parameters, lower mortality rate, and lower incidence of intestinal lesions. Flavonoids have been shown to have anti-bacterial, immuno-modulatory, and anti-inflammatory activity. The current study included four experimental groups: infected chickens fed commercial corn diet (CTRL-A) or PennHFD1 (CTRL-B) and infected chickens fed commercial corn diet (IF-A) or PennHFD1 (IF-B). We found that most of the microbiota changes were due to infection rather than diet. The alpha diversity in the IF chickens was lower in both CE-L and CE-M. The beta diversity of microbial communities was different between IF and CTRL chickens, as well as between CTRL-A and CTRL-B. The beta diversity of CTRL birds was more homogenous compared to IF samples. Taxonomic analysis showed a decrease in short chain fatty acid producing bacteria in IF birds. An increase in lactic acid producing bacteria, Escherichia coli, and Enterococcus cecorum was also observed in IF birds. It is possible that the effect of the high flavonoid corn on the microbiota was overcome by the effect of NE, or that the positive effects of increased flavonoids in NE-challenged birds are a result of mechanisms which do not involve the microbiota. The effects of high flavonoid corn on NE infections may be further investigated as a possible alternative to antimicrobials.
Data-driven approach for AI-based crack detection: techniques, challenges, and future scope
Authors: Chakurkar Priti S. , Vora Deepali , Patil Shruti , Mishra Sashikala , Kotecha Ketan
Published: October 2023

This article provides a systematic literature review on the application of artificial intelligence (AI) technology for detecting cracks in civil infrastructure, which is a critical issue affecting the performance and longevity of these structures. Traditional crack detection methods involve manual inspection, which is laborious and time-consuming, especially in urban areas. Therefore, automatic crack detection with AI technology has gained popularity due to its ability to identify degradation of roads in real-time, leading to increased safety and reliability. This review emphasizes two key approaches for crack detection: deep learning and traditional computer vision, with a focus on data-driven aspects that rely primarily on data from training datasets to detect and quantify the severity level of the crack. The article highlights the advantages and drawbacks of each approach and provides an overview of various crack detection models, feature extraction techniques, datasets, potential issues, and future directions. The research concludes that deep learning-based methods used for crack classification, localization and segmentation have shown better performance than traditional computer vision techniques, especially in terms of accuracy. However, deep learning methods require large amounts of training data and computational power, which can be a significant limitation. Additionally, the article identifies a lack of 3D datasets, unsupervised learning algorithms are rarely used to train crack detection model, and datasets having road images with variety of road textures such as asphalt and cement etc. as challenges for future research in this field. A need for 3D and combined texture datasets as challenges for future research in this field.
Menstrual Health: A Health and Human Rights Issue
Authors: Alarcão Violeta , Pintassilgo Sónia
Published: October 2023

Menstruation is a natural part of life, but for some people who menstruate it can be a reason for exclusion. Factors that contribute to exclusion include age, gender, poverty level, health conditions, and other societal factors. Menstrual health is a general state of wellbeing during the menstrual cycle, and it is important because everyone has the right to live their experiences with dignity and feel healthy. In this article, we discuss menstrual health, describe how certain groups face situations that can negatively impact their menstrual health, and explain why menstrual health should be a basic human right. We also discuss the links between menstruation and the environment and the roles that schools and everyone in society can play in contributing to menstrual justice, that is, participating in the debate surrounding menstrual health issues, and promoting healthy, inclusive, accessible, and sustainable menstrual management contexts.
Molecular characterization and phylogenetic analysis of highly pathogenic H5N1 clade 2.3.4.4b virus in Bosnia and Herzegovina
Authors: Goletic Sejla , Softic Adis , Omeragic Jasmin , Koro-Spahic Amira , Kapo Naida , Sabic Emina , Kasagic Dragan , Goletic Teufik
Published: October 2023

Influenza A virus continues to represent a growing problem affecting mainly birds but with an increasing number of mammal transmission events reported each year. Nevertheless, molecular characterization and phylogenetic analysis of influenza A viruses originating from all confirmed cases have not been systematically performed in all parts of the world. In this study, we investigated a sample originating from a mute swan that died in November 2021 in the northern part of Bosnia and Herzegovina with RT-qPCR and whole genome sequencing using ONT MinION. It was diagnosed as a highly pathogenic Influenza A virus, subtype H5N1 of 2.3.4.4b clade, and phylogenetic analysis revealed high sequence homology with other European HPAI H5N1 sequences at the time. The notable detected mutations in HA (N110S and T139P) and NA genes (H155Y), that facilitate the host specificity shift and enable the resistance to some antiviral drugs respectively, underscore the necessity of virus evolution surveillance. Therefore, the rapid dissemination of information, including virological and molecular data, is essential for the introduction of tailored prevention measures for infected animals, providing clearer insight and better awareness of a potential public health threat.
Agreement, Accuracy, and Reliability of a New Algorithm for the Detection of Change of Direction Angle Based on Integrating Inertial Data from Inertial Sensors
Authors: Avilés Roberto , Brito de Souza Diego , Pino-Ortega José , Castellano Julen
Published: October 2023

The development of algorithms applied to new technologies allows a better understanding of many of the movements in team sports. The purpose of this work was to analyze the validity, precision, and reproducibility of an algorithm to detect angulation of changes of direction (CoDs) while running, of between 45° and 180°, both to the left and the right at different speeds, in a standardized context. For this, five participants performed a total of 200 CoDs at 13 km/h and 128 CoDs at 18 km/h while wearing three inertial sensors. The information obtained from the sensors was contrasted with observation and coding using high-resolution video. Agreement between systems was assessed using Bland–Altman 95% limits of agreement as well as effect size (ES) and % difference between means. Reproducibility was evaluated using the standard error (CV%). The algorithm overestimated the angulation of 90° and 135° to the right (Cohen’s d > 0.91). The algorithm showed high precision when the angulations recorded at 13 km/h and 18 km/h were compared, except at 45° to the left (mean bias = −2.6°; Cohen’s d = −0.57). All angulations showed excellent reproducibility (CV < 5%) except at 45° (CV = 11%), which worsened when the pre-CoD speed was 18 km/h (CV < 16%). The algorithm showed a high degree of validity and reproducibility to detect angles during CoDs.
Factors Associated with the Prevalence and Treatment of Depression in Adolescent Males in the US during the Period of the COVID-19 Pandemic
Authors: Baser Onur , Zeng Yixuan , Alsaleh Sara , Baser Isabel
Published: October 2023

Background: Much of the research on the effect of the COVID-19 pandemic on mental health has overlooked the experiences of adolescent boys. Objective: To examine the prevalence of depression, treatment trends, and associated risk factors among adolescent boys, controlling for the pandemic year. Methods: Data for boys aged 12 to 17 years (n = 4518) in the 2021 National Survey on Drug Use and Health were analyzed. Time trends and factors associated with depression were examined using a multiple regression analysis. Results: The prevalence of 12-month major depressive episodes (TMDEs) was 11.6% during the first year of the COVID-19 pandemic. Among the boys with TMDEs, 37.8% received treatment overall, and 19.0% received prescription medication. Higher rates of TMDEs were estimated in boys who were older (adjusted odds ratio (AOR): 1.66, p < 0.001), lived in single-mother households (AOR: 1.47, p < 0.001), did not have authoritative parents (AOR: 1.78, p < 0.001), and had negative school experiences (AOR: 2.45, p < 0.001). Although Black boys were less likely to report depression than white boys (AOR: 0.70, p < 0.05), nonwhite boys who had depression were significantly less likely to receive treatment. Boys living in a household without a mother were also less likely to receive treatment (AOR: 0.49, p < 0.05). Conclusions: This study reveals the unique developmental, social, and psychological factors that influence depression among adolescent boys. During the pandemic, more than one out of ten adolescent boys had a major depressive episode, and four of ten of them received treatment; half of these treatments were prescription medication. Recognizing these factors may allow for more targeted and effective interventions to improve mental health outcomes for this demographic.
Evolution of Shock Waves during Muzzle Jet Impinging Moving Bodies under Different Constrained Boundaries
Authors: Li Zijie , Wang Hao
Published: October 2023

A recently developed launching device called the gun–track launch system is affected by its constrained track, such that the form of the muzzle jet changes from the state of free development in the entire space to a constrained state, where this lends unique characteristics of development to its flow field. In this study, the authors establish the corresponding model for numerical simulations based on the dynamic mesh method. We also considered a model of simulation of the muzzle jet with an “infinitely” constrained track to analyze its performance under real launch conditions to explore the mechanism of development and the disturbance-induced propagation of the shock wave when the muzzle jet impinges on moving bodies. The results showed that the muzzle jet exhibited a circumferential asymmetric shape that tilted toward the area above the muzzle and generated transverse air flow that led to the generation of a vortex on it. Because the muzzle was close to the ground, the jet was reflected by it to enhance the development and evolution of the shock waves and vortices and to aggravate the rate of distortion and asymmetry of the jet. The wave reflected from the ground was emitted once again when it encountered the infinitely constrained track. No local low-pressure area or a prominent vortex was observed after multiple reflections. Because the track in the test model was short, the waves reflected by the ground were not blocked, and vortices were formed in the area above the ground. Significant differences in the changes in pressure were also observed at key points in the domain. The results of a comparative analysis showed that the infinitely constrained track increased the Mach number of the moving body from 1.4 to 1.6. The work provides a theoretical basis and the requisite technical support for applications of the gun–track launch system.
Genome-Wide Association Analysis of Rice Leaf Traits
Authors: Wang Nansheng , Wang Xingmeng , Qian Yingzhi , Bai Di , Bao Yaling , Zhao Xueyu , Xu Peng , Li Keyang , Li Jianfeng , Li Kang , Zhang Dewen , Shi Yingyao
Published: October 2023

Yield-related traits have always been a research hotspot in rice breeding, and functional leaves directly affect the photosynthetic efficiency and yield of rice. Therefore, it is of great significance to explore the genes related to rice leaf size and shape to improve rice leaf morphology, photosynthesis efficiency, and yield. This study detected the quantitative trait loci (QTLs) for the length, width, length–width ratio, and area of rice flag leaf and second leaf in 393 accessions from the 3000 Rice Genome Project (3KRGP) by high-density single-nucleotide polymorphism genotyping. As a result, 91 QTLs were detected, among which 5 candidate genes (LOC_Os03g29170, LOC_Os06g17285, LOC_Os04g35060, LOC_Os03g27450, and LOC_Os09g16280) were identified. In addition, the epistatic interactions affecting leaf-related traits were also investigated, resulting in the identification of 134 significant QQIs (QTL by QTL interactions) pairs. The results of this study provide an important genetic basis for mining genes associated with rice leaf shape and valuable genetic information for rice breeding.
Prenatal Diagnosis of Canine and Feline Twins Using Ultrasound: A Retrospective Study
Authors: Pecchia Fabiana , Di Giorgio Stefania , Sfacteria Alessandra , Monti Salvatore , Vullo Cecilia , Catone Giuseppe , Marino Gabriele
Published: October 2023

Prenatal diagnosis comprises a set of investigations, both instrumental and laboratory-based, which aim to monitor the health of the foetus during pregnancy, from the early stages of embryonic development to the moments preceding delivery. A growing interest is emerging for the preterm ultrasound morphological screening of embryos and foetuses, aimed at assessing the integrity and viability of the conceptus, as well as the early diagnosis of anomalies which can cause complications. This study is a retrospective study of the ultrasonographic findings of twins in the authors’ clinical activity from 2016 to 2022. Only seven cases of monochorionic twins were recorded, out of the whole number of evaluations performed on 3120 foetuses, with a prevalence of 0.6% and 0.2% in feline and canine foetuses. All the twins had their own amniotic sac and umbilical cord but presented a single placenta and a single allantoic sac. Unfortunately, the three feline cases were not more recognizable at term. In the four canine cases, three were of opposite sex and then necessarily dizygotic. Twins may have an impact on the success of a pregnancy due to the risk of dystocia, as observed in some of the reported cases. Prenatal ultrasound allows early recognition of twins in dogs and cats.
The First Genome Survey of the Snail Provanna glabra Inhabiting Deep-Sea Hydrothermal Vents
Authors: Hui Min , Zhang Yu , Wang Aiyang , Sha Zhongli
Published: October 2023

The snail P. glabra is an endemic species in deep-sea chemosynthetic ecosystems of the Northwest Pacific Ocean. To obtain more genetic information on this species and provide the basis for subsequent whole-genome map construction, a genome survey was performed on this snail from the hydrothermal vent of Okinawa Trough. The genomic size of P. glabra was estimated to be 1.44 Gb, with a heterozygosity of 1.91% and a repeated sequence content of 69.80%. Based on the sequencing data, a draft genome of 1.32 Gb was assembled. Transposal elements (TEs) accounted for 40.17% of the entire genome, with DNA transposons taking the highest proportion. It was found that most TEs were inserted in the genome recently. In the simple sequence repeats, the dinucleotide motif was the most enriched microsatellite type, accounting for 53% of microsatellites. A complete mitochondrial genome of P. glabra with a total length of 16,268 bp was assembled from the sequencing data. After comparison with the published mitochondrial genome of Provanna sp. from a methane seep, 331 potential single nucleotide polymorphism (SNP) sites were identified in protein-coding genes (PCGs). Except for the cox1 gene, nad2, nad4, nad5, and cob genes are expected to be candidate markers for population genetic and phylogenetic studies of P. glabra and other deep-sea snails. Compared with shallow-water species, three mitochondrial genes of deep-sea gastropods exhibited a higher evolutionary rate, indicating strong selection operating on mitochondria of deep-sea species. This study provides insights into the genome characteristics of P. glabra and supplies genomic resources for further studies on the adaptive evolution of the snail in extreme deep-sea chemosynthetic environments.
First Capture of a Jaguar Using a Minimally Invasive Capture System for GPS Tracking in an Isolated Patch of Atlantic Forest in Southern Brazil
Authors: Palomares Francisco , de Paula Tarcízio Antônio Rego , Srbek-Araujo Ana Carolina
Published: October 2023

This study presents the first successful capture using GPS tagging of a jaguar (Panthera onca) using a minimally invasive capture system (MICS). We used snare-foot traps and a MICS during two capture campaigns in a fragment of Atlantic Forest in southeastern Brazil. The specimen disarmed snares on different occasions, and capture was only possible with the MICS. The captured jaguar, an estimated 16-year-old adult male, was monitored using a GPS Vertex Plus Iridium collar with an optimal performance of 86% in expected locations. The jaguar’s home range (659 km2 by MPC and 174 km2 by 95%K) was within the observed range for the species and the animal was primarily maintained in protected areas. The habitat types most frequently used were native grassland (27.2% of 4798 fixes), marsh (24.8%), and dense lowland forest (24.7%). The use of a MICS for trapping jaguars is a promising technique that shows advantages in terms of efficiency, selectivity, portability, reduced potential risk of injury to animals or trappers, and animal stress compared to other capture methods used for the species.
Heat Stress Mitigation: Impact of Increased Cooling Sessions on Milk Yield and Welfare of Dairy Buffaloes in a Semiarid Summer
Authors: Hussain Syed Israr , Ahmad Nisar , Ahmed Saeed , Akhter Maqsood , Shahid Muhammad Qamer
Published: October 2023

The current study aimed to evaluate the impact of increasing cooling sessions from three to five times a day on milk yield and the welfare of dairy buffaloes during a semiarid summer in Pakistan. Eighteen Nili Ravi buffaloes were randomly assigned to three cooling strategies: (1) CTL, where buffaloes were cooled with a handheld hosepipe twice daily for 5 min each; (2) 3CS, where buffaloes were cooled using sprinklers three times daily; and (3) 5CS, where buffaloes were cooled using sprinklers five times daily. Each sprinkler cooling session lasted 1 h, with a 6 min cycle of 3 min of water on and 3 min off. Results showed that the 5CS group produced 1.6 and 3.2 kg more milk per day compared to the 3CS and CTL groups, respectively (p < 0.001). Both the 5CS and 3CS groups had consistently lower core body temperatures and respiration rates than the CTL group. Buffaloes in the 5CS group spent significantly more time eating (p < 0.001). Additionally, the 5CS group exhibited lower cortisol and blood urea nitrogen levels (p = 0.001) and higher glucose levels than the CTL group (p = 0.006). In conclusion, increasing cooling sessions to five times daily improved milk yield and welfare compared to the traditional cooling strategy (CTL) in dairy buffaloes during semiarid summers, highlighting the benefits of optimized cooling practices.
Evaluation of the Immunoprotective Capacity of Five Vaccine Candidate Proteins against Avian Necrotic Enteritis and Impact on the Caecal Microbiota of Vaccinated Birds
Authors: Heidarpanah Sara , Thibodeau Alexandre , Parreira Valeria R. , Quessy Sylvain , Segura Mariela , Gottschalk Marcelo , Gaudreau Annie , Juette Tristan , Gaucher Marie-Lou
Published: October 2023

Avian necrotic enteritis is an enteric disease of broiler chickens caused by certain pathogenic strains of Clostridium perfringens in combination with predisposing factors. A vaccine offering complete protection against the disease has not yet been commercialized. In a previous study, we produced five recombinant proteins predicted to be surface-exposed and unique to necrotic enteritis-causing C. perfringens and the immunogenicity of these potential vaccine candidates was assessed in broiler chickens. In the current work, the relative contribution of the antibodies raised by these putative antigens to protect broiler chickens was evaluated using an experimental necrotic enteritis induction model. Additionally, the link between the immune response elicited and the gut microbiota profiles in immunized birds subjected to infection with virulent C. perfringens was studied. The ELISA results showed that the IgY antibody titers in vaccinated birds on days 21 and 33 were significantly higher than those on days 7 and 14 and those in birds receiving the adjuvant alone, while the relative contribution of the specific immunity attributed to these antibodies could not be precisely determined using this experimental necrotic enteritis induction model. In addition, 16S rRNA gene amplicon sequencing showed that immunization of birds with recombinant proteins had a low impact on the chicken caecal microbiota.
Assessing Optimal Cell Counts in Sperm Shape Abnormality Assays in Rodents
Authors: Cardoso Elizandra , Mathias Maria da Luz , Monarca Rita I. , Gabriel Sofia I.
Published: October 2023

Rodents have been the preferred models for the evaluation of the toxicity of pollutants and drugs and their genotoxic effects, including sperm shape abnormalities. The scientific literature is dominated by studies conducted with model animals in laboratory conditions, but a generally accepted and standardized protocol addressing the optimal number of sperm cells to count is still lacking. In this study, we reviewed the literature regarding the number of counted sperm cells in such assessments, published from 1969 to 2023. To infer the number of counts providing the best cost/benefit regarding the robustness of the assay results, a new dataset involving the analysis of two populations of wild rodents was produced. We evaluated the frequency of sperm shape abnormalities in a total of 50 wild brown rats (Rattus norvegicus) captured in two port cities, aiming to detect the impact of differential sperm cell counts in the obtained results. During necropsy, the fresh epididymis tail of adult male rats was excised, and sperm cells were fixated in slides. For each animal, a total of 300, 500, 1000, and 2000 cells were sequentially counted, and head abnormalities were registered. Counting 300 sperm cells failed to detect significant differences between groups and 500 counts resulted in marginally significant differences. Only when 1000 or 2000 sperm cells were counted, significant differences emerged between groups. We propose that studies addressing sperm shape abnormalities should standardize counts to an optimal value of 1000 cells per animal, warranting robust statistical results while providing the best compromise concerning labor time.
Human and Non-Human Primate Coexistence in Argentina: Conflicts and Solutions
Authors: García de la Chica Alba , Oklander Luciana I. , Kowalewski Martin M. , Fernandez-Duque Eduardo
Published: October 2023

There are five different primate species inhabiting widely distinct ecoregions in Argentina. Each of them faces various threats in terms of conservation and conflicts that hamper their ability to coexist with human populations. We present here some of the drivers known to be the causes of conflicts between humans and primates in the southernmost area of distribution of Latin American primates. We focus our synthesis on two of the biggest sources of conflict: the effects of different anthropogenic disturbances, and human misconceptions concerning the role of primates in the ecosystem. In each section, we briefly characterize the conflicts worldwide and then provide specific cases and examples from Argentina. In the last part of the manuscript, we further describe some ongoing national and regional educational, research, and conservation approaches to mitigate those effects.