bluuebunny/crossref_metadata_2025 huggingface dataset:
Aberrant Methylation Status of Tumour Suppressor Genes in Ovarian Cancer Tissue and Paired Plasma Samples
Authors: Dvorská Dana , Braný Dušan , Nagy Bálint , Grendár Marián , Poka Robert , Soltész Beáta , Jagelková Marianna , Zelinová Katarína , Lasabová Zora , Zubor Pavol , Danková Zuzana
Published: August 2019

Ovarian cancer is a highly heterogeneous disease and its formation is affected by many epidemiological factors. It has typical lack of early signs and symptoms, and almost 70% of ovarian cancers are diagnosed in advanced stages. Robust, early and non-invasive ovarian cancer diagnosis will certainly be beneficial. Herein we analysed the regulatory sequence methylation profiles of the RASSF1, PTEN, CDH1 and PAX1 tumour suppressor genes by pyrosequencing in healthy, benign and malignant ovarian tissues, and corresponding plasma samples. We recorded statistically significant higher methylation levels (p < 0.05) in the CDH1 and PAX1 genes in malignant tissues than in controls (39.06 ± 18.78 versus 24.22 ± 6.93; 13.55 ± 10.65 versus 5.73 ± 2.19). Higher values in the CDH1 gene were also found in plasma samples (22.25 ± 14.13 versus 46.42 ± 20.91). A similar methylation pattern with positive correlation between plasma and benign lesions was noted in the CDH1 gene (r = 0.886, p = 0.019) and malignant lesions in the PAX1 gene (r = 0.771, p < 0.001). The random forest algorithm combining methylation indices of all four genes and age determined 0.932 AUC (area under the receiver operating characteristic (ROC) curve) prediction power in the model classifying malignant lesions and controls. Our study results indicate the effects of methylation changes in ovarian cancer development and suggest that the CDH1 gene is a potential candidate for non-invasive diagnosis of ovarian cancer.
Comprehensive Analysis of CRISPR/Cas9-Mediated Mutagenesis in Arabidopsis thaliana by Genome-Wide Sequencing
Authors: Xu Wenjie , Fu Wei , Zhu Pengyu , Li Zhihong , Wang Chenguang , Wang Chaonan , Zhang Yongjiang , Zhu Shuifang
Published: August 2019

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been widely applied in functional genomics research and plant breeding. In contrast to the off-target studies of mammalian cells, there is little evidence for the common occurrence of off-target sites in plants and a great need exists for accurate detection of editing sites. Here, we summarized the precision of CRISPR/Cas9-mediated mutations for 281 targets and found that there is a preference for single nucleotide deletions/insertions and longer deletions starting from 40 nt upstream or ending at 30 nt downstream of the cleavage site, which suggested the candidate sequences for editing sites detection by whole-genome sequencing (WGS). We analyzed the on-/off-target sites of 6 CRISPR/Cas9-mediated Arabidopsis plants by the optimized method. The results showed that the on-target editing frequency ranged from 38.1% to 100%, and one off target at a frequency of 9.8%–97.3% cannot be prevented by increasing the specificity or reducing the expression level of the Cas9 enzyme. These results indicated that designing guide RNA with high specificity may be the preferred factor to avoid the off-target events, and it is necessary to predict or detect off-target sites by WGS-based methods for preventing off targets caused by genome differences in different individuals.
ZO-2 Is a Master Regulator of Gene Expression, Cell Proliferation, Cytoarchitecture, and Cell Size
Authors: González-Mariscal Lorenza , Gallego-Gutiérrez Helios , González-González Laura , Hernández-Guzmán Christian
Published: August 2019

ZO-2 is a cytoplasmic protein of tight junctions (TJs). Here, we describe ZO-2 involvement in the formation of the apical junctional complex during early development and in TJ biogenesis in epithelial cultured cells. ZO-2 acts as a scaffold for the polymerization of claudins at TJs and plays a unique role in the blood–testis barrier, as well as at TJs of the human liver and the inner ear. ZO-2 movement between the cytoplasm and nucleus is regulated by nuclear localization and exportation signals and post-translation modifications, while ZO-2 arrival at the cell border is triggered by activation of calcium sensing receptors and corresponding downstream signaling. Depending on its location, ZO-2 associates with junctional proteins and the actomyosin cytoskeleton or a variety of nuclear proteins, playing a role as a transcriptional repressor that leads to inhibition of cell proliferation and transformation. ZO-2 regulates cell architecture through modulation of Rho proteins and its absence induces hypertrophy due to inactivation of the Hippo pathway and activation of mTOR and S6K. The interaction of ZO-2 with viral oncoproteins and kinases and its silencing in diverse carcinomas reinforce the view of ZO-2 as a tumor regulator protein.
Antifibrotic Effects of High-Mobility Group Box 1 Protein Inhibitor (Glycyrrhizin) on Keloid Fibroblasts and Keloid Spheroids through Reduction of Autophagy and Induction of Apoptosis
Authors: Jeon Yeo Reum , Roh Hyun , Jung Ji Hyuk , Ahn Hyo Min , Lee Ju Hee , Yun Chae-Ok , Lee Won Jai
Published: August 2019

Overabundance of extracellular matrix resulting from hyperproliferation of keloid fibroblasts (KFs) and dysregulation of apoptosis represents the main pathophysiology underlying keloids. High-mobility group box 1 (HMGB1) plays important roles in the regulation of cellular death. Suppression of HMGB1 inhibits autophagy while increasing apoptosis. Suppression of HMGB1 with glycyrrhizin has therapeutic benefits in fibrotic diseases. In this study, we explored the possible involvement of autophagy and HMGB1 as a cell death regulator in keloid pathogenesis. We have highlighted the potential utility of glycyrrhizin as an antifibrotic agent via regulation of the aberrant balance between autophagy and apoptosis in keloids. Higher HMGB1 expression and enhanced autophagy were observed in keloids. The proliferation of KFs was decreased following glycyrrhizin treatment. While apoptosis was enhanced in keloids after glycyrrhizin treatment, autophagy was significantly reduced. The expressions of ERK1/2, Akt, and NF-κB, were enhanced in HMGB1-teated fibroblasts, but decreased following glycyrrhizin treatment. The expression of extracellular matrix (ECM) components was reduced in glycyrrhizin-treated keloids. TGF-β, Smad2/3, ERK1/2, and HMGB1 were decreased in glycyrrhizin-treated keloids. Treatment with the autophagy inhibitor 3-MA resulted in a decrease of autophagy markers and collagen in the TGF-β-treated fibroblasts. The results indicated that autophagy plays an important role in the pathogenesis of keloids. Because glycyrrhizin appears to reduce ECM and downregulate autophagy in keloids, its potential use for treatment of keloids is indicated.
Nuclear Disposition of Alien Chromosome Introgressions into Wheat and Rye Using 3D-FISH
Authors: Koláčková Veronika , Perničková Kateřina , Vrána Jan , Duchoslav Martin , Jenkins Glyn , Phillips Dylan , Turkosi Edina , Šamajová Olga , Sedlářová Michaela , Šamaj Jozef , Doležel Jaroslav , Kopecký David
Published: August 2019

During interphase, the chromosomes of eukaryotes decondense and they occupy distinct regions of the nucleus, called chromosome domains or chromosome territories (CTs). In plants, the Rabl’s configuration, with telomeres at one pole of nucleus and centromeres at the other, appears to be common, at least in plants with large genomes. It is unclear whether individual chromosomes of plants adopt defined, genetically determined addresses within the nucleus, as is the case in mammals. In this study, the nuclear disposition of alien rye and barley chromosomes and chromosome arm introgressions into wheat while using 3D-FISH in various somatic tissues was analyzed. All of the introgressed chromosomes showed Rabl’s orientation, but their relative positions in the nuclei were less clear. While in most cases pairs of introgressed chromosomes occupied discrete positions, their association (proximity) along their entire lengths was rare, and partial association only marginally more frequent. This arrangement is relatively stable in various tissues and during various stages of the cell cycle. On the other hand, the length of a chromosome arm appears to play a role in its positioning in a nucleus: shorter chromosomes or chromosome arms tend to be located closer to the centre of the nucleus, while longer arms are more often positioned at the nuclear periphery.
Oral Bacteria and Intestinal Dysbiosis in Colorectal Cancer
Authors: Koliarakis Ioannis , Messaritakis Ippokratis , Nikolouzakis Taxiarchis Konstantinos , Hamilos George , Souglakos John , Tsiaoussis John
Published: August 2019

The human organism coexists with its microbiota in a symbiotic relationship. These polymicrobial communities are involved in many crucial functions, such as immunity, protection against pathogens, and metabolism of dietary compounds, thus maintaining homeostasis. The oral cavity and the colon, although distant anatomic regions, are both highly colonized by distinct microbiotas. However, studies indicate that oral bacteria are able to disseminate into the colon. This is mostly evident in conditions such as periodontitis, where specific bacteria, namely Fusobacterium nucrelatum and Porphyromonas gingivalis project a pathogenic profile. In the colon these bacteria can alter the composition of the residual microbiota, in the context of complex biofilms, resulting in intestinal dysbiosis. This orally-driven disruption promotes aberrant immune and inflammatory responses, eventually leading to colorectal cancer (CRC) tumorigenesis. Understanding the exact mechanisms of these interactions will yield future opportunities regarding prevention and treatment of CRC.
Role of Cancer Stem Cells in Cholangiocarcinoma and Therapeutic Implications
Authors: Wu Hsing-Ju , Chu Pei-Yi
Published: August 2019

Cholangiocarcinoma (CCA) is the second most common type of liver cancer, and is highly aggressive with very poor prognosis. CCA is classified into intrahepatic cholangiocarcinoma (iCCA) and extra-hepatic cholangiocarcinoma (eCCA), which is further stratified into perihilar (pCCA) and distal (dCCA). Cancer stem cells (CSCs) are a subpopulation of cancer cells capable of tumor initiation and malignant growth, and are also responsible for chemoresistance. Thus, CSCs play an important role in CCA carcinogenesis. Surface markers such as CD133, CD24, CD44, EpCAM, Sox2, CD49f, and CD117 are important for identifying and isolating CCA CSCs. CSCs are present in the tumor microenvironment (TME), termed ‘CSC niche’, where cellular components and soluble factors interact to promote tumor initiation. Epithelial-to-mesenchymal transition (EMT) is another important mechanism underlying carcinogenesis, involved in the invasiveness, metastasis and chemoresistance of cancer. It has been demonstrated that EMT plays a critical role in generating CSCs. Therapies targeting the surface markers and signaling pathways of CCA CSCs, proteins involved in TME, and immune checkpoint proteins are currently under investigation. Therefore, this review focuses on recent studies on the roles of CSCs in CCA; the possible therapeutic strategies targeting CSCs of CCA are also discussed.
β-Glucose-1,6-Bisphosphate Stabilizes Pathological Phophomannomutase2 Mutants In Vitro and Represents a Lead Compound to Develop Pharmacological Chaperones for the Most Common Disorder of Glycosylation, PMM2-CDG
Authors: Monticelli Maria , Liguori Ludovica , Allocca Mariateresa , Andreotti Giuseppina , Cubellis Maria Vittoria
Published: August 2019

A large number of mutations causing PMM2-CDG, which is the most frequent disorder of glycosylation, destabilize phosphomannomutase2. We looked for a pharmacological chaperone to cure PMM2-CDG, starting from the structure of a natural ligand of phosphomannomutase2, α-glucose-1,6-bisphosphate. The compound, β-glucose-1,6-bisphosphate, was synthesized and characterized via 31P-NMR. β-glucose-1,6-bisphosphate binds its target enzyme in silico. The binding induces a large conformational change that was predicted by the program PELE and validated in vitro by limited proteolysis. The ability of the compound to stabilize wild type phosphomannomutase2, as well as frequently encountered pathogenic mutants, was measured using thermal shift assay. β-glucose-1,6-bisphosphate is relatively resistant to the enzyme that specifically hydrolyses natural esose-bisphosphates.
Epigenetics and Neurological Disorders in ART
Authors: La Rovere Marina , Franzago Marica , Stuppia Liborio
Published: August 2019

About 1–4% of children are currently generated by Assisted Reproductive Technologies (ART) in developed countries. These babies show only a slightly increased risk of neonatal malformations. However, follow-up studies have suggested a higher susceptibility to multifactorial, adult onset disorders like obesity, diabetes and cardiovascular diseases in ART offspring. It has been suggested that these conditions could be the consequence of epigenetic, alterations, due to artificial manipulations of gametes and embryos potentially able to alter epigenetic stability during zygote reprogramming. In the last years, epigenetic alterations have been invoked as a possible cause of increased risk of neurological disorders, but at present the link between epigenetic modifications and long-term effects in terms of neurological diseases in ART children remains unclear, due to the short follow up limiting retrospective studies. In this review, we summarize the current knowledge about neurological disorders promoted by epigenetics alterations in ART. Based on data currently available, it is possible to conclude that little, if any, evidence of an increased risk of neurological disorders in ART conceived children is provided. Most important, the large majority of reports appears to be limited to epidemiological studies, not providing any experimental evidence about epigenetic modifications responsible for an increased risk.
FOXG1-Related Syndrome: From Clinical to Molecular Genetics and Pathogenic Mechanisms
Authors: Wong Lee-Chin , Singh Shekhar , Wang Hsin-Pei , Hsu Chia-Jui , Hu Su-Ching , Lee Wang-Tso
Published: August 2019

Individuals with mutations in forkhead box G1 (FOXG1) belong to a distinct clinical entity, termed “FOXG1-related encephalopathy”. There are two clinical phenotypes/syndromes identified in FOXG1-related encephalopathy, duplications and deletions/intragenic mutations. In children with deletions or intragenic mutations of FOXG1, the recognized clinical features include microcephaly, developmental delay, severe cognitive disabilities, early-onset dyskinesia and hyperkinetic movements, stereotypies, epilepsy, and cerebral malformation. In contrast, children with duplications of FOXG1 are typically normocephalic and have normal brain magnetic resonance imaging. They also have different clinical characteristics in terms of epilepsy, movement disorders, and neurodevelopment compared with children with deletions or intragenic mutations. FOXG1 is a transcriptional factor. It is expressed mainly in the telencephalon and plays a pleiotropic role in the development of the brain. It is a key player in development and territorial specification of the anterior brain. In addition, it maintains the expansion of the neural proliferating pool, and also regulates the pace of neocortical neuronogenic progression. It also facilitates cortical layer and corpus callosum formation. Furthermore, it promotes dendrite elongation and maintains neural plasticity, including dendritic arborization and spine densities in mature neurons. In this review, we summarize the clinical features, molecular genetics, and possible pathogenesis of FOXG1-related syndrome.
Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
Authors: Vernen Felicitas , Harvey Peta J. , Dias Susana A. , Veiga Ana Salomé , Huang Yen-Hua , Craik David J. , Lawrence Nicole , Troeira Henriques Sónia
Published: August 2019

Tachyplesin I, II and III are host defense peptides from horseshoe crab species with antimicrobial and anticancer activities. They have an amphipathic β-hairpin structure, are highly positively-charged and differ by only one or two amino acid residues. In this study, we compared the structure and activity of the three tachyplesin peptides alongside their backbone cyclized analogues. We assessed the peptide structures using nuclear magnetic resonance (NMR) spectroscopy, then compared the activity against bacteria (both in the planktonic and biofilm forms) and a panel of cancerous cells. The importance of peptide-lipid interactions was examined using surface plasmon resonance and fluorescence spectroscopy methodologies. Our studies showed that tachyplesin peptides and their cyclic analogues were most potent against Gram-negative bacteria and melanoma cell lines, and showed a preference for binding to negatively-charged lipid membranes. Backbone cyclization did not improve potency, but improved peptide stability in human serum and reduced toxicity toward human red blood cells. Peptide-lipid binding affinity, orientation within the membrane, and ability to disrupt lipid bilayers differed between the cyclized peptide and the parent counterpart. We show that tachyplesin peptides and cyclized analogues have similarly potent antimicrobial and anticancer properties, but that backbone cyclization improves their stability and therapeutic potential.
Survey on Drosophila suzukii Natural Short-Term Dispersal Capacities Using the Mark−Release−Recapture Technique
Authors: Vacas Sandra , Primo Jaime , Manclús Juan J. , Montoya Ángel , Navarro-Llopis Vicente
Published: August 2019

Spotted wing drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae), has become a key pest for soft fruits and cherries in Europe in less than a decade since the first outbreak in 2007. Although this pest’s passive dispersal ability has been observed over more than 1400 km in 1 year, active spread has not yet been extensively studied. A mark−release−recapture (MRR) method based on protein-marked flies was employed to determine the flight capacity of D. suzukii. Sterile marked flies were released and recaptured in a trap grid at increasing distances from 10 to 250 m from the releasing point to study flight distance during periods ranging from 3 h to 1 week. MRR experiments were replicated in the presence and absence of host fruits to study how they could affect dispersal behavior. The dispersal capacity of the Mediterranean fruit fly, Ceratitis capitata Wiedemann (Diptera: Tephritidae) was also studied under the same conditions. The results showed a low dispersal ability for D. suzukii, with a daily flight distance below 100 m with no predominant wind. The implications on natural dispersion and control methods based on attractants are discussed.
A Tutorial on Robust Control, Adaptive Control and Robust Adaptive Control—Application to Robotic Manipulators
Authors: Wei Bin
Published: August 2019

A tutorial on robust control, adaptive control, robust adaptive control and adaptive control of robotic manipulators is presented in a systematic manner. Some limitations of the above methods are also illustrated. The relationships between the robust control, adaptive control and robust adaptive control are demonstrated. Basic information on the joint space control, operational space control and force control is also given. This tutorial summarizes the most advanced control techniques currently in use in a very simple manner, and applies to robotic manipulators, which can provide an informative guideline for students who have little knowledge of controls or who want to understand the adaptive control of robotics in a systematic way.
Lipoprotein(a) Gene Polymorphism Increases a Risk Factor for Aortic Valve Calcification
Authors: Ozkan Ugur , Ozcelik Fatih , Yildiz Mustafa , Budak Metin
Published: August 2019

Calcific aortic valve disease (CAVD) is a multifactorial condition. Both environmental and genetic factors play an important role in its etiology. CAVD exhibits a broad spectrum, varying from mild valve thickening to severe valve calcification and stenosis. Progression of the disease consists of chronic inflammation, lipoprotein deposition, and active leaflet calcification. It is a process similar to coronary artery disease. In this study, we investigated Lp(a) levels and gene polymorphisms associated with calcific aortic stenosis from blood samples after echocardiography in the evaluation of 75 patients diagnosed with CAVD and 77 controls. Blood tests were run in our laboratory to rule out certain risk factors before echocardiography examination. A significant association among smoking, elevated LDL level and creatinine, low albumin levels, Lp(a) level, rs10455872, and rs3798220 polymorphisms may be considered genetic risk factors for the development of calcific aortic stenosis.
Biopsy Confirmed Glioma Recurrence Predicted by Multi-Modal Neuroimaging Metrics
Authors: Costabile Jamie D. , Thompson John A. , Alaswad Elsa , Ormond D. Ryan
Published: August 2019

Histopathological verification is currently required to differentiate tumor recurrence from treatment effects related to adjuvant therapy in patients with glioma. To bypass the complications associated with collecting neural tissue samples, non-invasive classification methods are needed to alleviate the burden on patients while providing vital information to clinicians. However, uncertainty remains as to which tissue features on magnetic resonance imaging (MRI) are useful. The primary objective of this study was to quantitatively assess the reliability of combining MRI and diffusion tensor imaging metrics to discriminate between tumor recurrence and treatment effects in histopathologically identified biopsy samples. Additionally, this study investigates the noise adjuvant radiation therapy introduces when discriminating between tissue types. In a sample of 41 biopsy specimens, from a total of 10 patients, we derived region-of-interest samples from MRI data in the ipsilateral hemisphere that encompassed biopsies obtained during resective surgery. This study compares normalized intensity values across histopathology classifications and contralesional volumes reflected across the midline. Radiation makes noninvasive differentiation of abnormal-nontumor tissue to tumor recurrence much more difficult. This is because radiation exhibits opposing behavior on key MRI modalities: specifically, on post-contrast T1, FLAIR, and GFA. While radiation makes noninvasive differentiation of tumor recurrence more difficult, using a novel analysis of combined MRI metrics combined with clinical annotation and histopathological correlation, we observed that it is possible to successfully differentiate tumor tissue from other tissue types. Additional work will be required to expand upon these findings.
Physical Activity Levels Are Low in Inoperable Lung Cancer: Exploratory Analyses from a Randomised Controlled Trial
Authors: Edbrooke Lara , Granger Catherine L. , Clark Ross A. , Denehy Linda
Published: August 2019

Background: In inoperable lung cancer, evidence is limited regarding physical activity (PA) and associations with other outcomes. Aims: in the usual care (UC) group of an RCT to (1) explore whether baseline PA was associated with improved follow-up outcomes, (2) identify baseline variables associated with higher follow-up PA and in all RCT participants, to (3) analyse patterns of objectively measured PA, and (4) report on characteristics of those who were able to maintain or increase PA levels. Methods: exploratory analyses of an assessor-blinded RCT. Outcomes, assessed at baseline, nine weeks and six months, included PA (seven-days of accelerometry), six-minute walk distance (6MWD), muscle strength, symptoms, mood and health-related quality of life (HRQoL). Results: 92 participants were randomised, 80 completed baseline accelerometry (39 intervention group (IG), 41 UC), characteristics: mean (SD) age 63.0 (12.3) years, 56% male, 51% stage IV disease. Baseline PA: median (IQR) steps/day 2859.6 (2034.0–3849.2) IG versus 3195.2 (2161.2–4839.0) UC. Associations between baseline PA and six-month outcomes were significant for HRQoL and 6MWD. PA at six months was significantly associated with baseline age, 6MWD and quadriceps strength. Between-group change score (steps/day) mean differences (95% CI) at nine weeks (174.5 (−1504.7 to 1853.7), p = 0.84) and six months (574.0 (−1162.3 to 2310.3), p = 0.52). Conclusions: further research is required to determine patient subgroups deriving the greatest benefits from PA interventions.
Erratum: Hohlstein, P. et al. Prognostic Relevance of Altered Lymphocyte Subpopulations in Critical Illness and Sepsis. Journal of Clinical Medicine 2019, 8, 353
Authors: Hohlstein Philipp , Gussen Hendrik , Bartneck Matthias , Warzecha Klaudia , Roderburg Christoph , Buendgens Lukas , Trautwein Christian , Koch Alexander , Tacke Frank
Published: August 2019

The authors wish to make the following corrections to this paper [...]
N-Glycoproteomic Profiling Reveals Alteration In Extracellular Matrix Organization In Non-Type Bladder Carcinoma
Authors: Deb Barnali , Patel Krishna , Sathe Gajanan , Kumar Prashant
Published: August 2019

Treatment of advanced and metastatic bladder carcinoma is often ineffective and displays variable clinical outcomes. Studying this aggressive molecular subtype of bladder carcinoma will lead to better understanding of the pathogenesis which may lead to the identification of new therapeutic strategies. The non-type bladder subtype is phenotypically mesenchymal and has mesenchymal features with a high metastatic ability. Post-translational addition of oligosaccharide residues is an important modification that influences cellular functions and contributes to disease pathology. Here, we report the comparative analysis of N-linked glycosylation across bladder cancer subtypes. To analyze the glycosite-containing peptides, we carried out LC-MS/MS-based quantitative proteomic and glycoproteomic profiling. We identified 1299 unique N-linked glycopeptides corresponding to 460 proteins. Additionally, we identified 118 unique N-linked glycopeptides corresponding to 84 proteins to be differentially glycosylated only in non-type subtypes as compared to luminal/basal subtypes. Most of the altered glycoproteins were also observed with changes in their global protein expression levels. However, alterations in 55 differentially expressed glycoproteins showed no significant change at the protein abundance level, representing that the glycosylation site occupancy was changed between the non-type subtype and luminal/basal subtypes. Importantly, the extracellular matrix organization pathway was dysregulated in the non-type subtype of bladder carcinoma. N-glycosylation modifications in the extracellular matrix organization proteins may be a contributing factor for the mesenchymal aggressive phenotype in non-type subtype. These aberrant protein glycosylation would provide additional avenues to employ glycan-based therapies and may lead to the identification of novel therapeutic targets.
The Effect of Tapered Abutments on Marginal Bone Level: A Retrospective Cohort Study
Authors: Marconcini Simone , Giammarinaro Enrica , Covani Ugo , Mijiritsky Eitan , Vela Xavier , Rodríguez Xavier
Published: August 2019

Background: Early peri-implant bone loss has been associated to long-term implant-prosthetic failure. Different technical, surgical, and prosthetic techniques have been introduced to enhance the clinical outcome of dental implants in terms of crestal bone preservation. The aim of the present cohort study was to observe the mean marginal bone level around two-part implants with gingivally tapered abutments one year after loading. Methods: Mean marginal bone levels and change were computed following radiological calibration and linear measurement on standardized radiographs. Results: Twenty patients who met the inclusion criterion of having at least one implant with the tapered prosthetic connection were included in the study. The cumulative implant success rate was 100%, the average bone loss was −0.18 ± 0.72 mm, with the final bone level sitting above the implant platform most of the time (+1.16 ± 0.91 mm). Conclusion: The results of this cohort study suggested that implants with tapered abutments perform successfully one year after loading and that they are associated with excellent marginal bone preservation, thus suggesting that implant-connection macro-geometry might have a crucial role in dictating peri-implant bone levels.
Comparison of the Processing of Epoxy Resins in Pultrusion with Open Bath Impregnation and Closed-Injection Pultrusion
Authors: Strauß Sebastian , Senz Andreas , Ellinger Jessica
Published: August 2019

In this study, the influence of the open bath and closed-injection pultrusion (CIP) processing methods of epoxy resins on the quality of glass fiber composites was investigated. In addition to the state-of-the-art epoxy resin system with long pot life, new resin systems with short pot life have recently been developed. These systems require processing by closed-injection pultrusion. The epoxies with long pot life allow both processing variants. The experimental work was carried out with two types of injection and impregnation chambers (ii_chamber), namely with a conical and a teardrop design. Fully impregnated composites, which were used for further analyses, could be produced by using the conical ii_chamber. The composite properties of the open bath and the conical ii_chamber impregnation methods were compared. No significant influence on the bending stress could be determined; the interlaminar shear strength was up to 10% better with open bath impregnation than with ii_chamber. For the composites investigated, it was shown that the open bath and ii_chamber impregnation methods can be used to produce parts with partially comparable properties, as demonstrated for the epoxy formulation with long pot life. These results indicate that processing of epoxy systems with a short pot life is also possible by closed-injection pultrusion. Furthermore, the influence on the composite properties of the time interval between the mixing of an epoxy resin and processing in an open bath was investigated. No significant effect on the bending stress and interlaminar shear strength could be determined.
Elastics Selector Gauge as Orthodontics Device Applied to Inter-Maxillary Traction during Malocclusion Correction
Authors: Sambataro Sergio , Bocchieri Salvatore , Bafumi Luigi , Fiorillo Luca , Cervino Gabriele , Cicciù Marco
Published: August 2019

Elastics are the simplest device that can be used during a class correction in orthodontics, and despite the simplicity of a latex band, they are very effective and powerful. The resultant inter-maxillary force affects not only the teeth, but even the mandibular position, and consequently the temporomandibular joints (TMJ). The purpose of our work is to simplify the use of elastics, and to reduce the amount of inventory for orthodontists, because there is a lot of merceology available on the market, and different ways of using the elastics. The use of elastics in clinical practice is based on the force extension values, which are given by the manufacturer for the different sizes of the elastics, generally when they are stretched to three times their lumen size. Various configurations allow for the correction of different malocclusions. We propose a new classification and a new device, the elastic selector gauge, in order to allow clinicians to quickly and easily choose the right elastic in all conditions.
Harmful Fouling Communities on Fish Farms in the SW Mediterranean Sea: Composition, Growth and Reproductive Periods
Authors: Bosch-Belmar Mar , Escurriola Agnés , Milisenda Giacomo , Fuentes Verónica L. , Piraino Stefano
Published: August 2019

Biological fouling organisms on fish cages represent a major issue and costly factor in marine finfish aquaculture. Cnidarians have been identified as one of the most problematical groups, contributing significantly to the occlusion and structural stress of the cage nets, but also dramatically affecting farmed species health in aquaculture facilities worldwide. Recently, significant relationships were established in different Spanish aquaculture facilities between hydrozoans and juvenile fish affected by gill injuries and mortality episodes. Community composition, growth rate and reproductive potential of biofouling were monitored on fish cages over two seasonal periods of fry cages farming, located in southern Spain (SW Alboran Sea), with a special focus on cnidarians. Biomass and community composition of biofouling changed with time and between studied periods, with a marked seasonality in colonization periods and taxonomic composition, particularly for the colonial hydrozoans. The hydroids Ectopleura larynx and Pennaria disticha were found at the highest densities. P. disticha was responsible for major biomass contribution to total hydroid biomass with the fastest growth rates. In addition, actinulae larvae of E. larynx were identified in zooplankton samples at high densities especially during periods of fry introduction in sea cages (when fish are highly vulnerable). These results corroborate evidence of the detrimental influence of fouling cnidarians in Mediterranean finfish aquaculture due to a direct harmful impact on fish health. Investigations on population dynamics, reproductive biology and envenomation potential of fouling hydrozoans should be regarded as key component of best monitoring practices to ensure good farmed fish welfare, maximization of aquaculture production and overall marine spatial planning.
Land Use Planning for Natural Hazards
Authors: Bathrellos George D. , Skilodimou Hariklia D.
Published: August 2019

The Earth’s landscape has a complex evolution and is the result of the interactions involving surficial processes, climate, tectonic, and human activity [...]
Wall-to-Wall Parcel-Level Mapping of Agricultural Land Abandonment in the Polish Carpathians
Authors: Kolecka Natalia , Kozak Jacek
Published: August 2019

Accurate estimations of the extent of agricultural land abandonment (ALA) are critical to the sustainable management of agricultural resources and forestry, the understanding of ALA determinants, and the development of future agricultural policies. Although ALA is widespread in Europe, mapping it over large areas using remote sensing data is difficult as a result of the complexity of this phenomenon. This study aims to develop methods for a detailed wall-to-wall regional-scale mapping of ALA using vegetation height and secondary forest succession indicators. The rates and distribution of ALA were analyzed at the parcel and communal level in the Polish Carpathians using a high-resolution vegetation height model (VHM) derived from Light Detection and Ranging (LiDAR) point clouds and topographic data. Depending on the parcel-level secondary forest succession threshold (10, 20, and 50%), the regional ALA rates were 18.8, 9.0, and 2.1%, respectively. Regardless of the threshold, abandoned grasslands covered about three times more area than abandoned croplands. The highest ALA rates were observed in communes located in the western part of the study area, as well as east and south of Rzeszów. We found that areas receiving European Union Common Agricultural Policy payments very rarely showed signs of secondary forest succession and land abandonment. The developed method proved to be effective for detailed ALA mapping at various spatial scales.
Educating Refugee-Background Students: Critical Issues and Dynamic Contexts. Edited by Shawna Shapiro, Raichle Farrelly and Mary Jane Curry, Bristol: Multilingual Matters, 2018, 264p. ISBN: 978-1-78309-996-2
Authors: Isserlis Janet
Published: August 2019

Educators, scholars and practitioners whose work intersects with refugee learners’ lives and schooling are familiar with the many strengths and challenges these learners bring to and encounter in classrooms and communities every day [...]
End Coercion in Mental Health Services—Toward a System Based on Support Only
Authors: Zinkler Martin , von Peter Sebastian
Published: August 2019

Based on the UN Convention on the Rights of Persons with Disabilities (CRPD), several UN bodies, among them the High Commissioner for Human Rights, have argued for a complete ban of all coercive interventions in mental health care. The authors conceptualize a system for mental health care based on support only. Psychiatry loses its function as an agent of social control and follows the will and preferences of those who require support. The authors draw up scenarios for dealing with risk, inpatient care, police custody, and mental illness in prison. With such a shift, mental health services could earn the trust of service users and thereby improve treatment outcomes.
Bioprinting Vasculature: Materials, Cells and Emergent Techniques
Authors: Tomasina Clarissa , Bodet Tristan , Mota Carlos , Moroni Lorenzo , Camarero-Espinosa Sandra
Published: August 2019

Despite the great advances that the tissue engineering field has experienced over the last two decades, the amount of in vitro engineered tissues that have reached a stage of clinical trial is limited. While many challenges are still to be overcome, the lack of vascularization represents a major milestone if tissues bigger than approximately 200 µm are to be transplanted. Cell survival and homeostasis is to a large extent conditioned by the oxygen and nutrient transport (as well as waste removal) by blood vessels on their proximity and spontaneous vascularization in vivo is a relatively slow process, leading all together to necrosis of implanted tissues. Thus, in vitro vascularization appears to be a requirement for the advancement of the field. One of the main approaches to this end is the formation of vascular templates that will develop in vitro together with the targeted engineered tissue. Bioprinting, a fast and reliable method for the deposition of cells and materials on a precise manner, appears as an excellent fabrication technique. In this review, we provide a comprehensive background to the fields of vascularization and bioprinting, providing details on the current strategies, cell sources, materials and outcomes of these studies.
Physicochemical Study on the Strength Development Characteristics of Cold Weather Concrete Using a Nitrite–Nitrate Based Accelerator
Authors: Choi Heesup , Inoue Masumi , Choi Hyeonggil , Kim Jihoon , Sudoh Yuhji , Kwon Sukmin , Lee Bokyeong , Yoneyama Akira
Published: August 2019

There has recently been an increased use of anti-freezing agents that are primarily composed of salt- and alkali-free calcium nitrite (Ca(NO2)2) and calcium nitrate (Ca(NO3)2) to promote the hydration reaction of concrete in cold weather concreting. Nitrite–nitrate based accelerators accelerate the hydration of C3A and C3S in cement more quickly when their quantities are increased, thereby boosting the concrete’s early strength and effectively preventing early frost damage. However, the connection between the hydrate formation behavior and the strength development characteristic over time has yet to be clearly identified. Therefore, in this study, a wide range of physicochemical reviews were carried out to clarify the relationship between the hydrate formation behavior and the strength development characteristics, both at an early age and at later ages, which results from the addition of nitrite–nitrate based accelerators to concrete in varying amounts. These accelerators also act as anti-freezing agents. The results show that an increased quantity of nitrite–nitrate based accelerators caused an increase in the early strength of the concrete. This was due to the formation of nitrite and nitrate hydrates in large amounts, in addition to ettringite containing SO42, which is generated during the hydration reaction of normal Portland cement at an early age. On the other hand, at later ages, there was a rise in nitrite and nitrate hydrates with needle crystal structures exhibiting brittle fracture behavior. A decrease in C–S–H gel and Ca(OH)2 hydrates, deemed to have caused a decline in strength on Day 3 and thereafter, was also observed.
3D Microscale Heat Transfer Model of the Thermal Properties of Wood-Metal Functional Composites Based on the Microstructure
Authors: Chai Yuan , Liang Shanqing , Zhou Yongdong , Lin Lanying , Fu Feng
Published: August 2019

This study presents a model for simulating the microscopic heat transfer processes in a wood-metal composite material. The model was developed by analyzing the microstructure of experimental samples comprising a melted alloy impregnated in a wood matrix. According to the thermal parameters of the materials and the boundary conditions, an analytical model of microscale heat transfer was established using Abaqus finite element analysis software. The model was validated experimentally by comparing temperature curves obtained via simulation and experiments; the resulting correlation coefficient was 0.96557. We then analyzed the temperature distribution of the composite material with different cell geometries and heat transfer conditions (heat transfer direction and applied temperature). The thermal properties of the unit cell models were in good agreement with the general trends predicted by several heat transfer equations. This study provides a method for analyzing the microscale heat transfer process in wood-based composites. In addition, the model framework characteristics can be used to evaluate the heat transfer mechanism of impregnated modified wood.
Cuttlefish Bone-Derived Biphasic Calcium Phosphate Scaffolds Coated with Sol-Gel Derived Bioactive Glass
Authors: Neto Ana S. , Brazete Daniela , Ferreira José M.F.
Published: August 2019

The combination of calcium phosphates with bioactive glasses (BG) has received an increased interest in the field of bone tissue engineering. In the present work, biphasic calcium phosphates (BCP) obtained by hydrothermal transformation of cuttlefish bone (CB) were coated with a Sr-, Mg- and Zn-doped sol-gel derived BG. The scaffolds were characterized by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. The initial CB structure was maintained after hydrothermal transformation (HT) and the scaffold functionalization did not jeopardize the internal structure. The results of the in-vitro bioactivity after immersing the BG coated scaffolds in simulated body fluid (SBF) for 15 days showed the formation of apatite on the surface of the scaffolds. Overall, the functionalized CB derived BCP scaffolds revealed promising properties, but further assessment of the in-vitro biological properties is needed before being considered for their use in bone tissue engineering applications.
Wire Ropes and CFRP Strips to Provide Masonry Walls with Out-Of-Plane Strengthening
Authors: Ferretti Elena
Published: August 2019

The present paper deals with an improvement of the strengthening technique consisting in the combined use of straps—made of stainless steel ribbons—and CFRP (Carbon Fiber Reinforced Polymer) strips, to increase the out-of-plane ultimate load of masonry walls. The straps of both the previous and the new combined technique pass from one face to the opposite face of the masonry wall through some holes made along the thickness, giving rise to a three-dimensional net of loop-shaped straps, closed on themselves. The new technique replaces the stainless steel ribbons with steel wire ropes, which form closed loops around the masonry units and the CFRP strips as in the previous technique. A turnbuckle for each steel wire rope allows the closure of the loops and provides the desired pre-tension to the straps. The mechanical coupling—given by the frictional forces—between the straps and the CFRP strips on the two faces of the masonry wall gives rise to an I-beam behavior that forces the CFRP strips to resist the load as if they were the two flanges of the same I-beam. Even the previous combined technique exploits the ideal I-beam mechanism, but the greater stiffness of the steel wire ropes compared to the stiffness of the steel ribbons makes the constraint between the facing CFRP strips stiffer. This gives the reinforced structural element a greater stiffness and delamination load. In particular, the experimental results show that the maximum load achievable with the second combined technique is much greater than the maximum load provided by the CFRP strips. Even the ultimate displacement turns out to be increased, allowing us to state that the second combined technique improves both strength and ductility. Since the CFRP strips of the combined technique run along the vertical direction of the wall, the ideal I-beam mechanism is particularly useful to counteract the hammering action provided by the floors on the perimeter walls, during an earthquake. Lastly, when the building suffers heavy structural damage due to a strong earthquake, the box-type behavior offered by the three-dimensional net of straps prevents the building from collapsing, acting as a device for safeguarding life.
Influence of Binder Property and Mortar Thickness on High-Temperature Performance of Cold Recycled Mixtures with Asphalt Emulsion
Authors: Gao Lei , Wang Zhanqi , Liu Yanping , Zheng Junqiu , Li Hua
Published: August 2019

Four kinds of cold recycling (CR) mixtures with different asphalt emulsions were studied for their high-temperature performance in both binder properties and internal structures aspects. Digital image processing was introduced to determine the thickness spectrum for the asphalt mortar of the CR mixtures from a mesoscopic perspective. The time–temperature sweep (TTS) test was conducted to obtain the rheological parameters of each corresponding emulsified residue and the permanent deformation performance of each CR mixture was measured by dynamic creep test. A principle component analysis (PCA) was used to compare the typical performance parameters of the CR mixtures and find the factors controlling the rutting resistance of CR mixtures. The results show that the high-temperature performance of the CR mixtures with a modified emulsified asphalt showed improvements relative to the nominal case. Including Marshall stability, several parameters from the rheological properties of binder (G*/sinδ, flow number) and mortar thickness (max, range proportion 0–10 mm) could significantly influence the high-temperature performance and rutting resistance of the CR mixtures.
Influence of Interfacial Traps on the Operating Temperature of Perovskite Solar Cells
Authors: Mehdizadeh-Rad Hooman , Singh Jai
Published: August 2019

In this paper, by developing a mathematical model, the temperature of PSCs under different operating conditions has been calculated. It is found that by reducing the density of tail states at the interfaces through some passivation mechanisms, the operating temperature can be decreased significantly at higher applied voltages. The results show that if the density of tail states at the interfaces is reduced by three orders of magnitude through some passivation mechanisms, then the active layer may not undergo any phase change up to an ambient temperature 300 K and it may not degrade up to 320 K. The calculated heat generation at the interfaces at different applied voltages with and without passivation shows reduced heat generation after reducing the density of tail states at the interfaces. It is expected that this study provides a deeper understanding of the influence of interface passivation on the operating temperature of PSCs.