bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibition of exosome biogenesis affects cell motility in heterogeneous sub-populations of paediatric-type diffuse high-grade gliomas
Authors: Pericoli Giulia , Galardi Angela , Paolini Alessandro , Petrilli Lucia Lisa , Pepe Gerardo , Palma Alessandro , Colletti Marta , Ferretti Roberta , Giorda Ezio , Levi Mortera Stefano , Burford Anna , Carai Andrea , Mastronuzzi Angela , Mackay Alan , Putignani Lorenza , Jones Chris , Pascucci Luisa , Peinado Hector , Helmer-Citterich Manuela , de Billy Emmanuel , Masotti Andrea , Locatelli Franco , Di Giannatale Angela , Vinci Maria
Published: November 2023

Background

Paediatric-type diffuse High-Grade Gliomas (PDHGG) are highly heterogeneous tumours which include distinct cell sub-populations co-existing within the same tumour mass. We have previously shown that primary patient-derived and optical barcoded single-cell-derived clones function as interconnected networks. Here, we investigated the role of exosomes as a route for inter-clonal communication mediating PDHGG migration and invasion.

Results

A comprehensive characterisation of seven optical barcoded single-cell-derived clones obtained from two patient-derived cell lines was performed. These analyses highlighted extensive intra-tumour heterogeneity in terms of genetic and transcriptional profiles between clones as well as marked phenotypic differences including distinctive motility patterns. Live single-cell tracking analysis of 3D migration and invasion assays showed that the single-cell-derived clones display a higher speed and longer travelled distance when in co-culture compared to mono-culture conditions. To determine the role of exosomes in PDHGG inter-clonal cross-talks, we isolated exosomes released by different clones and characterised them in terms of marker expression, size and concentration. We demonstrated that exosomes are actively internalized by the cells and that the inhibition of their biogenesis, using the phospholipase inhibitor GW4689, significantly reduced the cell motility in mono-culture and more prominently when the cells from the clones were in co-culture. Analysis of the exosomal miRNAs, performed with a miRNome PCR panel, identified clone-specific miRNAs and a set of miRNA target genes involved in the regulation of cell motility/invasion/migration. These genes were found differentially expressed in co-culture versus mono-culture conditions and their expression levels were significantly modulated upon inhibition of exosome biogenesis.

Conclusions

In conclusion, our study highlights for the first time a key role for exosomes in the inter-clonal communication in PDHGG and suggests that interfering with the exosome biogenesis pathway may be a valuable strategy to inhibit cell motility and dissemination for these specific diseases.
Use of airway pressure-based indices to detect high and low inspiratory effort during pressure support ventilation: a diagnostic accuracy study
Authors: Yang Yan-Lin , Liu Yang , Gao Ran , Song De-Jing , Zhou Yi-Min , Miao Ming-Yue , Chen Wei , Wang Shu-Peng , Wang Yue-Fu , Zhang Linlin , Zhou Jian-Xin
Published: November 2023

Background

Assessment of the patient’s respiratory effort is essential during assisted ventilation. We aimed to evaluate the accuracy of airway pressure (Paw)-based indices to detect potential injurious inspiratory effort during pressure support (PS) ventilation.

Methods

In this prospective diagnostic accuracy study conducted in four ICUs in two academic hospitals, 28 adult acute respiratory failure patients undergoing PS ventilation were enrolled. A downward PS titration was conducted from 20 cmH2O to 2 cmH2O at a 2 cmH2O interval. By performing an end-expiratory airway occlusion maneuver, the negative Paw generated during the first 100 ms (P0.1) and the maximal negative swing of Paw (∆Pocc) were measured. After an end-inspiratory airway occlusion, Paw reached a plateau, and the magnitude of change in plateau from peak Paw was measured as pressure muscle index (PMI). Esophageal pressure was monitored and inspiratory muscle pressure (Pmus) and Pmus–time product per minute (PTPmus/min) were used as the reference standard for the patient’s effort. High and low effort was defined as Pmus > 10 and < 5 cmH2O, or PTPmus/min > 200 and < 50 cmH2O s min−1, respectively.

Results

A total of 246 levels of PS were tested. The low inspiratory effort was diagnosed in 145 (59.0%) and 136 (55.3%) PS levels using respective Pmus and PTPmus/min criterion. The receiver operating characteristic area of the three Paw-based indices by the respective two criteria ranged from 0.87 to 0.95, and balanced sensitivity (0.83–0.96), specificity (0.74–0.88), and positive (0.80–0.91) and negative predictive values (0.78–0.94) were obtained. The high effort was diagnosed in 34 (13.8%) and 17 (6.9%) support levels using Pmus and PTPmus/min criterion, respectively. High receiver operating characteristic areas of the three Paw-based indices by the two criteria were found (0.93–0.95). A high sensitivity (0.80–1.00) and negative predictive value (0.97–1.00) were found with a low positive predictive value (0.23–0.64).

Conclusions

By performing simple airway occlusion maneuvers, the Paw-based indices could be reliably used to detect low inspiratory efforts. Non-invasive and easily accessible characteristics support their potential bedside use for avoiding over-assistance. More evaluation of their performance is required in cohorts with high effort.
Title-plus-abstract versus title-only first-level screening approach: a case study using a systematic review of dietary patterns and sarcopenia risk to compare screening performance
Authors: Teo Lynn , Van Elswyk Mary E. , Lau Clara S. , Shanahan Christopher J.
Published: November 2023

Background

Conducting a systematic review is a time- and resource-intensive multi-step process. Enhancing efficiency without sacrificing accuracy and rigor during the screening phase of a systematic review is of interest among the scientific community.

Methods

This case study compares the screening performance of a title-only (Ti/O) screening approach to the more conventional title-plus-abstract (Ti + Ab) screening approach. Both Ti/O and Ti + Ab screening approaches were performed simultaneously during first-level screening of a systematic review investigating the relationship between dietary patterns and risk factors and incidence of sarcopenia. The qualitative and quantitative performance of each screening approach was compared against the final results of studies included in the systematic review, published elsewhere, which used the standard Ti + Ab approach. A statistical analysis was conducted, and contingency tables were used to compare each screening approach in terms of false inclusions and false exclusions and subsequent sensitivity, specificity, accuracy, and positive predictive power.

Results

Thirty-eight citations were included in the final analysis, published elsewhere. The current case study found that the Ti/O first-level screening approach correctly identified 22 citations and falsely excluded 16 citations, most often due to titles lacking a clear indicator of study design or outcomes relevant to the systematic review eligibility criteria. The Ti + Ab approach correctly identified 36 citations and falsely excluded 2 citations due to limited population and intervention descriptions in the abstract. Our analysis revealed that the performance of the Ti + Ab first-level screening was statistically different compared to the average performance of both approaches (Chi-squared: 5.21, p value 0.0225) while the Ti/O approach was not (chi-squared: 2.92, p value 0.0874). The predictive power of the first-level screening was 14.3% and 25.5% for the Ti/O and Ti + Ab approaches, respectively. In terms of sensitivity, 57.9% of studies were correctly identified at the first-level screening stage using the Ti/O approach versus 94.7% by the Ti + Ab approach.

Conclusions

In the current case study comparing two screening approaches, the Ti + Ab screening approach captured more relevant studies compared to the Ti/O approach by including a higher number of accurately eligible citations. Ti/O screening may increase the likelihood of missing evidence leading to evidence selection bias.

Systematic review registration

PROSPERO Protocol Number: CRD42020172655.
Experimental and Analytical Investigation of Fracture Characteristics of Steel Fiber-Reinforced Recycled Aggregate Concrete
Authors: Maglad Ahmed M. , Mansour Walid , Tayeh Bassam A. , Elmasry Mohamed , Yosri Ahmed M. , Fayed Sabry
Published: November 2023

Fracture parameters of fiber concrete (FC) are currently a hot research area. Fracture mechanics is the field of solid mechanics that helps to study the type and propagation of cracks in materials. It uses methods of calculating the driving force on a crack and characterizes the material's resistance to fracture. Behavioral characteristics are determined by crack mouth opening displacement and the load–deflection method. This research identifies the fracture parameters of 33 notched simply supported beams made by recycled aggregate cement concrete with steel fiber. The recycled aggregate ratio in concrete has been altered to determine the effect on the mechanical and fracture properties. For determining fracture parameters, a 3-point bending single-edge notched fracture test was used. The results indicated that the steel fiber-reinforced concrete made with recycled aggregate showed similar performance and fracture characteristics compared to normal concrete. Thus, adding steel fibers to various concrete mixes considerably improved the fracture characteristics, while the brittleness was reduced with increased steel fiber content. Linear regression analysis also showed the accuracy of mechanical strength results as the value of R-square was close to unity. Displacement, ultimate load, brittleness (B), fracture toughness (KIC), crack mouth opening displacement (CMOD), fracture energy (GF), modulus of elasticity (E), and characteristic length (lch), were determined for both conventional and recycled aggregate specimens. The “work of fracture"—by definition the formula—is the most reliable to calculate the fracture energy as the nonlinearity is related to the performance of FC.
Maternal microbiota communicates with the fetus through microbiota-derived extracellular vesicles
Authors: Kaisanlahti Anna , Turunen Jenni , Byts Nadiya , Samoylenko Anatoliy , Bart Genevieve , Virtanen Nikke , Tejesvi Mysore V. , Zhyvolozhnyi Artem , Sarfraz Sonia , Kumpula Sohvi , Hekkala Jenni , Salmi Sonja , Will Olga , Korvala Johanna , Paalanne Niko , Erawijantari Pande Putu , Suokas Marko , Medina Tuula Peñate , Vainio Seppo , Medina Oula Peñate , Lahti Leo , Tapiainen Terhi , Reunanen Justus
Published: November 2023

Background

Reports regarding the presence of bacteria in the fetal environment remain limited and controversial. Recently, extracellular vesicles secreted by the human gut microbiota have emerged as a novel mechanism for host-microbiota interaction. We aimed to investigate the presence of bacterial extracellular vesicles in the fetal environment during healthy pregnancies and determine whether extracellular vesicles derived from the gut microbiota can cross biological barriers to reach the fetus.

Results

Bacterial extracellular vesicles were detectable in the amniotic fluid of healthy pregnant women, exhibiting similarities to extracellular vesicles found in the maternal gut microbiota. In pregnant mice, extracellular vesicles derived from human maternal gut microbiota were found to reach the intra-amniotic space.

Conclusions

Our findings reveal maternal microbiota-derived extracellular vesicles as an interaction mechanism between the maternal microbiota and fetus, potentially playing a pivotal role in priming the prenatal immune system for gut colonization after birth.
Autofluorescence imaging of endogenous metabolic cofactors in response to cytokine stimulation of classically activated macrophages
Authors: Bess Shelby N. , Igoe Matthew J. , Denison Abby C. , Muldoon Timothy J.
Published: November 2023

Background

Macrophages are one of the most prevalent subsets of immune cells within the tumor microenvironment and perform a range of functions depending on the cytokines and chemokines released by surrounding cells and tissues. Recent research has revealed that macrophages can exhibit a spectrum of phenotypes, making them highly plastic due to their ability to alter their physiology in response to environmental cues. Recent advances in examining heterogeneous macrophage populations include optical metabolic imaging, such as fluorescence lifetime imaging (FLIM), and multiphoton microscopy. However, the method of detection for these systems is reliant upon the coenzymes NAD(P)H and FAD, which can be affected by factors other than cytoplasmic metabolic changes. In this study, we seek to validate these optical measures of metabolism by comparing optical results to more standard methods of evaluating cellular metabolism, such as extracellular flux assays and the presence of metabolic intermediates.

Methods

Here, we used autofluorescence imaging of endogenous metabolic co-factors via multiphoton microscopy and FLIM in conjunction with oxygen consumption rate and extracellular acidification rate through Seahorse extracellular flux assays to detect changes in cellular metabolism in quiescent and classically activated macrophages in response to cytokine stimulation.

Results

Based on our Seahorse XFP flux analysis, M0 and M1 macrophages exhibit comparable trends in oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). Autofluorescence imaging of M0 and M1 macrophages was not only able to show acute changes in the optical redox ratio from pre-differentiation (0 hours) to 72 hours post-cytokine differentiation (M0: 0.320 to 0.258 and M1: 0.316 to 0.386), mean NADH lifetime (M0: 1.272 ns to 1.379 ns and M1: 1.265 ns to 1.206 ns), and A1/A2 ratio (M0: 3.452 to ~ 4 and M1: 3.537 to 4.529) but could also detect heterogeneity within each macrophage population.

Conclusions

Overall, the findings of this study suggest that autofluorescence metabolic imaging could be a reliable technique for longitudinal tracking of immune cell metabolism during activation post-cytokine stimulation.
Turning the tide: understanding estuarine detection range variability via structural equation models
Authors: Bruneel Stijn , Goossens Jolien , Reubens Jan , Pauwels Ine , Moens Tom , Goethals Peter , Verhelst Pieterjan
Published: November 2023

Insight into the detection range of acoustic telemetry systems is crucial for both sampling design and data interpretation. The detection range is highly dependent on the environmental conditions and can consequently be substantially different among aquatic systems. Also within systems, temporal variability can be significant. The number of studies to assess the detection range in different systems has been growing, though there remains a knowledge gap in estuarine habitats. In this study, a 2-month experimental set-up was used to assess the detection range variability and affecting environmental factors of an estuary. Given the expected complex interplay of different factors and the difficulties it entails for interpretation, a structural equation modelling (pSEM) approach is proposed. The detection range of this estuarine study was relatively low and variable (average 50% detectability of 106 m and ranging between 72 and 229 m) compared to studies of riverine and marine systems. The structural equation models revealed a clear, yet complex, tidal pattern in detection range variability which was mainly affected by water speed (via ambient noise and tilt of the receivers), water depth and wind speed. The negative effect of ambient noise and positive effect of water depth became more pronounced at larger distances. Ambient noise was not only affected by water speed, but also by water depth, precipitation, tilt angle and wind speed. Although the tilt was affected by water speed, water depth and wind speed, most of the variability in tilt could be traced back to the receiver locations. Similarly, the receiver locations seemed to explain a considerable portion of the detection range variability. Retrospective power analyses indicated that for most factors only a minor gain in explanatory power was achieved after more than two days of data collecting. Redirecting some of the sampling effort towards more spatially extensive measurements seems to be a relevant manner to improve the insights in the performance of telemetry systems in estuarine environments. Since the low and variable detection range in estuaries can seriously hamper ecological inferences, range tests with sound sampling designs and appropriate modelling techniques are paramount.
The 4-item family resilience scale: psychometric evaluation and measurement invariance of the malay version in adolescents and young adults
Authors: Hamzah Hazalizah , Tan Chee-Seng , Ramlee Fatanah , Zulkifli Syara Shazanna
Published: November 2023

Background

The original Family Resilience Scale (FRS) is a reliable tool to assess family resilience. However, the FRS is based on the United States and parental context. Thus, the usefulness of the FRS for the adolescent and young adult population in Asian countries, particularly Malaysia remains unknown. This study translated the FRS into the Malay language and validated it on Malaysian adolescents and young adults to identify its potential as a self-report tool to assess the resilience level of their family.

Methods

A total of 351 participants (Mage = 19.75, SDage = 3.29) were recruited in the study using purposive sampling. Confirmatory factor analysis was conducted to examine the factorial structure of the Family Resilience Scale-Malay (FRS-Malay) and measurement invariance between adolescents and young adults. Then, the scale’s reliability was investigated using Cronbach’s alpha, McDonald’s omega coefficients, and composite reliability index. Finally, we examined the discriminant validity of the FRS-Malay by correlating its score with individual resilience score and examined the incremental validity of the scale using hierarchical multiple regression analysis to test if family resilience can explain individual well-being levels beyond and above individual resilience.

Results

The findings of the confirmatory factor analysis suggest that a single-factor model is supported for both age groups. Furthermore, the scale exhibited scalar invariance between adolescents and young adults. The scale also exhibited good reliability, as the value of Cronbach’s alpha, McDonald omega coefficients, and composite reliability index were above 0.80. Additionally, the Pearson correlation analysis showed a positive correlation between the FRS-Malay and individual resilience scores, which supports the discriminant validity of the scale. Similarly, the incremental validity of the scale is also supported. Specifically, family resilience had a positive correlation with well-being, even after controlling for individual resilience in the regression analysis.

Conclusions

The FRS-Malay has demonstrated good reliability and validity. The scale measures the same construct of family resilience across adolescents and young adults, making it suitable for comparisons. Therefore, this unidimensional tool is appropriate for self-reporting their perceived level of family resilience. It is also useful for studying the development and fluctuation of family resilience in the Malaysian context.
Exploring the feasibility of using long-term stored newborn dried blood spots to identify metabolic features for congenital heart disease screening
Authors: Ceresnak Scott R. , Zhang Yaqi , Ling Xuefeng B. , Su Kuo Jung , Tang Qiming , Jin Bo , Schilling James , Chou C. James , Han Zhi , Floyd Brendan J. , Whitin John C. , Hwa Kuo Yuan , Sylvester Karl G , Chubb Henry , Luo Ruben Y. , Tian Lu , Cohen Harvey J. , McElhinney Doff B.
Published: November 2023

Congenital heart disease (CHD) represents a significant contributor to both morbidity and mortality in neonates and children. There’s currently no analogous dried blood spot (DBS) screening for CHD immediately after birth. This study was set to assess the feasibility of using DBS to identify reliable metabolite biomarkers with clinical relevance, with the aim to screen and classify CHD utilizing the DBS. We assembled a cohort of DBS datasets from the California Department of Public Health (CDPH) Biobank, encompassing both normal controls and three pre-defined CHD categories. A DBS-based quantitative metabolomics method was developed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). We conducted a correlation analysis comparing the absolute quantitated metabolite concentration in DBS against the CDPH NBS records to verify the reliability of metabolic profiling. For hydrophilic and hydrophobic metabolites, we executed significant pathway and metabolite analyses respectively. Logistic and LightGBM models were established to aid in CHD discrimination and classification. Consistent and reliable quantification of metabolites were demonstrated in DBS samples stored for up to 15 years. We discerned dysregulated metabolic pathways in CHD patients, including deviations in lipid and energy metabolism, as well as oxidative stress pathways. Furthermore, we identified three metabolites and twelve metabolites as potential biomarkers for CHD assessment and subtypes classifying. This study is the first to confirm the feasibility of validating metabolite profiling results using long-term stored DBS samples. Our findings highlight the potential clinical applications of our DBS-based methods for CHD screening and subtype classification.
Glass beads from a Scythian grave on the island of Khortytsia (Zaporizhzhia, Ukraine): insights into bead making through 3D imaging
Authors: Nykonenko Dmytro , Yatsuk Oleh , Guidorzi Laura , Lo Giudice Alessandro , Tansella Francesca , Cesareo Ludovica Pia , Sorrentino Giusi , Davit Patrizia , Gulmini Monica , Re Alessandro
Published: November 2023

Four glass beads from a Scythian burial on the island of Khortytsia (Southern Ukraine) were subjected to 3D imaging using micro-CT and photogrammetry. The aim was to reconstruct the process used to produce and decorate the beads by detecting and interpreting the traces left by the technological processes on the bead surface and in the glass body. It turned out that all the beads were obtained by winding hot glass around the mandrel. The distribution, size and shape of the bubbles in the glass matrix revealed by the micro-CT scans and the features observed during a thorough examination of the photogrammetric models allowed us to follow the movements of the bead maker during the formation of the bead body and its decoration, highlighting several details of the production processes such as the number of the superimposed layers and the direction of the rotation of the mandrel during both the formation of the body and the decoration of the bead. Some information about the tools also emerged, with particular reference to the shape of the mandrel, the possible use of a releasing agent and how tools were used to decorate the surface or to remove the beads from the mandrel. According to the archaeological classification, the beads considered here belong to three different types, that are considered chronological indicators of the fourth century BCE and are found in archaeological sites spread over an area extending for several thousand kilometers from the Black Sea coast to the Ural Mountains. This work enriches the knowledge of the micromorphology of beads found in Eastern Europe, which is rarely discussed in the scientific literature on the archaeological glass beads.
Behavioral patterns in collaborative problem solving: a latent profile analysis based on response times and actions in PISA 2015
Authors: Han Areum , Krieger Florian , Borgonovi Francesca , Greiff Samuel
Published: November 2023

Process data are becoming more and more popular in education research. In the field of computer-based assessments of collaborative problem solving (ColPS), process data have been used to identify students’ test-taking strategies while working on the assessment, and such data can be used to complement data collected on accuracy and overall performance. Such information can be used to understand, for example, whether students are able to use a range of styles and strategies to solve different problems, given evidence that such cognitive flexibility may be important in labor markets and societies. In addition, process information might help researchers better identify the determinants of poor performance and interventions that can help students succeed. However, this line of research, particularly research that uses these data to profile students, is still in its infancy and has mostly been centered on small- to medium-scale collaboration settings between people (i.e., the human-to-human approach). There are only a few studies involving large-scale assessments of ColPS between a respondent and computer agents (i.e., the human-to-agent approach), where problem spaces are more standardized and fewer biases and confounds exist. In this study, we investigated students’ ColPS behavioral patterns using latent profile analyses (LPA) based on two types of process data (i.e., response times and the number of actions) collected from the Program for International Student Assessment (PISA) 2015 ColPS assessment, a large-scale international assessment of the human-to-agent approach. Analyses were conducted on test-takers who: (a) were administered the assessment in English and (b) were assigned the Xandar unit at the beginning of the test. The total sample size was N = 2,520. Analyses revealed two profiles (i.e., Profile 1 [95%] vs. Profile 2 [5%]) showing different behavioral characteristics across the four parts of the assessment unit. Significant differences were also found in overall performance between the profiles.
Simple, sensitive, and label-free miRNA analysis through strand displacement reaction integrating with G-quadruplex-based signal generation
Authors: Zhang Hongmin , Li Jie , Huang Hong , Wang Hao , Qiu Sheng , Yang Gangyi
Published: November 2023

MicroRNAs (miRNAs) play a significant role in the pathogenesis of various diseases throughout biological processes, and the accurate detection of miRNA biomarkers holds great potential for early stage disease diagnosis and treatment. In this study, a novel method is developed to detect miRNA-21, a biomarker for drug-induced liver injury, by combining target sequence recycling with G-quadruplex-based signal production. This approach is highly sensitive and does not require the use of labels. The target sequence facilitates the cyclic exposure of G-rich regions in the detection probe by toehold-mediated strand displacement processes, with the aid of the catalytic chain. The G-quadruplex sequences that have been produced subsequently interact with thioflavin T (ThT), resulting in a significant increase in its fluorescence intensity. This enhanced fluorescence is utilized for the purpose of detecting miRNA-21, with a remarkably low detection limit of 4.4 fM. The suggested technique also allows for the very specific identification of the target miRNA-21. Due to its non-label format, excellent selectivity, and sensitivity, this technology presents a straightforward and versatile approach for detecting a wide range of biomarkers in the early phases of illness detection.
Efficacy, safety, and pharmacokinetics of MR13A11A, a generic of remifentanil, for pain management of Japanese patients in the intensive care unit: a double-blinded, fentanyl-controlled, randomized, non-inferiority phase 3 study
Authors: Doi Matsuyuki , Takahashi Naoki , Nojiri Rumi , Hiraoka Takehiko , Kishimoto Yusuke , Inoue Shinichi , Oya Nobuyo
Published: November 2023

Background

The aims of this study were to evaluate the efficacy, safety, and pharmacokinetics (PK) of continuous intravenous administration of remifentanil in mechanically ventilated patients in the intensive care unit (ICU).

Methods

This was a multicenter, randomized, double-blinded, fentanyl-controlled, non-inferiority phase 3 study. Patients aged ≥ 20 years requiring 6 h to 10 days mechanical ventilation in an ICU and requiring pain relief were randomly assigned in a 1:1 ratio to receive either remifentanil (n = 98) or fentanyl (n = 98). Dose was titrated from an infusion rate of 1 mL/h (remifentanil: 0.025 µg/kg/min, fentanyl: 0.1 µg/kg/h) until the target level of analgesia (behavioral pain scale [BPS] ≤ 5 or numerical rating score [NRS] ≤ 3) was achieved by escalating the dose in 1 mL/h increasing. Administration was then adjusted to maintain the target level of analgesia until weaning from the ventilator. The primary endpoint was the proportion of patients who did not require rescue fentanyl. Safety was assessed according to standard procedures. PK of remifentanil in the arterial blood was assessed in 24 patients.

Results

The proportion of patients achieving the primary endpoint in the remifentanil and fentanyl groups was 100% (92/92) and 97.8% (88/90), respectively. The difference between the groups was 2.2% (95% confidence interval, − 0.8–5.3) and non-inferiority of remifentanil to fentanyl was verified (p < 0.0001). The incidences of any adverse events in the remifentanil and fentanyl groups was 34 of 92 patients (37.0%) and 34 of 90 patients (37.8%), respectively. Adverse drug reactions was 12 in 92 patients (13.0%) and 15 in 90 patients (16.7%), respectively. In the PK analysis, blood remifentanil concentration decreased within 10 min to almost 50% of the end of administration, suggesting rapid offset of action following discontinuation of remifentanil.

Conclusions

Remifentanil can be used safely for pain management in mechanically ventilated Japanese patients in the ICU.

Trial registration: Japan Registry of Clinical Trials, jRCT2080224954. Registered 20 November 2019, https://jrct.niph.go.jp/latest-detail/jRCT2080224954.
Statistical characterization of full-margin rupture recurrence for Cascadia subduction zone using event time resampling and Gaussian mixture model
Authors: Goda Katsuichiro
Published: November 2023

Earthquake occurrence modeling of large subduction events involves significant uncertainty, stemming from the scarcity of geological data and inaccuracy of dating techniques. The previous research on statistical modeling of full-margin ruptures of the Cascadia subduction zone attempted to address these issues. However, the adopted resampling method to account for the uncertain marine turbidite age data from the Cascadia subduction zone was not sufficient in the sample size. This study presents a statistical approach based on the Gaussian mixture model applied to significantly larger resampled Cascadia age data. The results suggest that the 3-component Gaussian mixture model outperforms the 2-component Gaussian mixture model and the 1-component renewal models by capturing the long gap and short-term clustering. The developed Gaussian mixture model is well suited to apply to probabilistic seismic and tsunami hazard analysis and the calculation of long-term probability of the future full-margin Cascadia events by considering the elapsed time since the last event.
The gut microbiome mediates adaptation to scarce food in Coleoptera
Authors: Moldovan Oana Teodora , Carrell Alyssa A. , Bulzu Paul-Adrian , Levei Erika , Bucur Ruxandra , Sitar Cristian , Faur Luchiana , Mirea Ionuț Cornel , Șenilă Marin , Cadar Oana , Podar Mircea
Published: November 2023

Beetles are ubiquitous cave invertebrates worldwide that adapted to scarce subterranean resources when they colonized caves. Here, we investigated the potential role of gut microbiota in the adaptation of beetles to caves from different climatic regions of the Carpathians. The beetles’ microbiota was host-specific, reflecting phylogenetic and nutritional adaptation. The microbial community structure further resolved conspecific beetles by caves suggesting microbiota-host coevolution and influences by local environmental factors. The detritivore species hosted a variety of bacteria known to decompose and ferment organic matter, suggesting turnover and host cooperative digestion of the sedimentary microbiota and allochthonous-derived nutrients. The cave Carabidae, with strong mandibula, adapted to predation and scavenging of animal and plant remains, had distinct microbiota dominated by symbiotic lineages Spiroplasma or Wolbachia. All beetles had relatively high levels of fermentative Carnobacterium and Vagococcus involved in lipid accumulation and a reduction of metabolic activity, and both features characterize adaptation to caves.
Beyond emergency remote teaching: did the pandemic lead to lasting change in university courses?
Authors: Broadbent J. , Ajjawi R. , Bearman M. , Boud D. , Dawson P.
Published: November 2023

The COVID-19 pandemic significantly disrupted traditional methods of teaching and learning within higher education. But what remained when the pandemic passed? While the majority of the literature explores the shiftsduringthe pandemic, with much speculation about post-pandemic futures, a clear understanding of lasting implications remains elusive. To illuminate this knowledge gap, our study contrasts pedagogical practices in matched courses from the pre-pandemic year (2019) to the post-pandemic phase (2022/2023). We also investigate the factors influencing these changes and the perceptions of academics on these shifts. Data were gathered from academics in a large comprehensive Australian university of varying disciplines through a mixed-methods approach, collecting 67 survey responses and conducting 21 interviews. Findings indicate a notable increase in online learning activities, authentic and scaffolded assessments, and online unsupervised exams post-pandemic. These changes were primarily driven by university-guided adaptations, time and workload pressures, continued COVID-19 challenges, local leadership, an individual desire to innovate, and concerns about academic integrity. While most changes were seen as favourable by academics, perceptions were less positive concerning online examinations. These findings illuminate the enduring effects of the pandemic on higher education, suggesting longer-term implications than previous studies conducted during the acute phase of the pandemic.
“Adaptive virtual synchronous generator control using optimized bang-bang for Islanded microgrid stability improvement”
Authors: Elwakil Mahmoud M. , Zoghaby Helmy M. El , Sharaf Soliman M. , Mosa Magdi A.
Published: November 2023

In this paper, a virtual synchronous generator (VSG) controller is applied to a hybrid energy storage system (HESS) containing a battery energy storage system and supercapacitor storage system for maintaining the frequency stability of an isolated microgrid. The microgrid contains a photovoltaic generation system and a diesel generator in addition to the HESS and two constant impedance loads that are fed through a medium voltage radial feeding system. The adaptive virtual inertia constant (H) with constant virtual damping coefficient (D) based on ‘ bang-bang’ control for the microgrid’s frequency stability enhancement is investigated and compared with the constant parameter VSG. In addition, the bang-bang control is modified to adapt the D beside the adaptive H, and the system response is investigated and compared with the conventional adaptive H technique. The VSG parameters are evaluated based on two different methods. The first is a computational method based on the simplified small signal stability analysis, while the other is based on an optimization method using two different objective functions and the particle swarm optimization technique. This paper also investigates the superiority of the proposed technique compared to other techniques in enhancing frequency stability, accelerating steady-state frequency restoration, and reducing the energy requirement of the HESS. The required power from the HESS is shared between the two energy storages using the low pass filter technique so as to reduce battery peak current.
Intraoperative ultrasonographic-guided surgery of intracerebral hemorrhage
Authors: Sultan Ahmed Elsayed , Elwany Amr , Madkour Amr , Agamy Mohmed
Published: November 2023

Background

The location of the cortical incision and maximum evacuations of parenchymal intracerebral hematoma are crucial points. Intraoperative ultrasonography is a real-time tool with great benefit at these points

Methods

A retrospective study of patients with parenchymal intracerebral hemorrhage that underwent evacuation using intraoperative ultrasound guidance was included. Preoperative clinical and radiological assessments were studied. The postoperative imaging and clinical outcome were assessed. A late follow-up after 6 months was done.

Results

The age of study patients ranged from 9 to 73 (mean 45.3 ± 20.4 years). There were 14 males (58.3%). The mean preoperative Glasgow Coma Scale (GCS) was 9.8 ± 2.9. The preoperative hematoma volume ranged from 32 to 135 cm3 with a mean of 68.5 ± 30.5 cm3. The rate of evacuation ranged from 90 to 100%. The mean postoperative GCS was 11.7 ± 2.5. None of the patients had a recurrent hemorrhage. There was a significant rate of evacuation of the hematomas (P value < 0.001*). Also, there was a marked improvement in postoperative GCS and the late modified Rankin Scale (P value < 0.001*).

Conclusion

Ultrasonography is a useful, efficient, real-time tool for the localization and evacuation of parenchymal intracerebral hemorrhage. It maximizes evacuation and decreases parenchymal insult. It is an important aid to the neurosurgeon.
Evaluation of ovarian reserve in unexplained ınfertile cases: a case-controlled study
Authors: Sahin Mustafa , Guven Suleyman , Sal Hidayet , Guvendag Guven Emine Seda
Published: November 2023

Background

The aim of this study was to evaluate the effectiveness of current ovarian reserve tests in unexplained infertile cases.

Material and methods

This case–control study was conducted on 70 unexplained infertile women who were included in a tertiary university hospital. Both groups of basal FSH, estradiol, antimullerian hormone (AMH), inhibin B, ovarian volume, total antral follicle count (AFC), ovarian volume, and ovarian stromal blood flow (peak systolic velocity (PSV), S/D (systole and diastole ratio), resistance index (RI), and pulsatility index (PI)) values were compared.

Results

The mean AMH, inhibin B, PSV, and stromal blood flow values of the control group patients were higher than those in the unexplained infertility group. However, the values of the means of RI and PI of the cases in the control group were lower than those in the infertility group. When PI’s value was ≥ 2.00, its sensitivity was 65.7%, and its specificity was 64.3%. In the case of the RI, its value was ≥ 0.745, its sensitivity was 65.7%, and its specificity was 62.9%. In order to show ovarian reserve in unexplained infertile cases, the sensitivity values can be sorted from high to low as follows: PI > RI > estradiol > FSH > ovarian volume > AFC > inhibin B > stromal blood flow > PSV > S/D.

Conclusion

In the unexplained infertile patient group with normal ovarian reserve test results, basal estradiol, decreased PI, and RI values may be used as good ovarian reserve predictors.
IMP2ART: development of a multi-level programme theory integrating the COM-B model and the iPARIHS framework, to enhance implementation of supported self-management of asthma in primary care
Authors: Steed Liz , Sheringham Jessica , McClatchey Kirstie , Hammersley Vicky , Marsh Viv , Morgan Noelle , Jackson Tracy , Holmes Steve , Taylor Stephanie , Pinnock Hilary
Published: November 2023

Background

Supported asthma self-management, incorporating an asthma action plan and annual clinical review, has been recommended by UK/global guidelines for over three decades. However, implementation remains poor, as only around a third of individuals receive basic asthma care, according to the UKs leading respiratory charity Asthma and Lung UK. A systematic review of implementation studies recommended that a whole systems approach targeting patients, healthcare professional education, and organisations is needed to improve implementation of supported asthma self-management in primary care. The IMPlementing IMProved Asthma self-management as RouTine (IMP2ART) is a national Hybrid-II implementation cluster randomised controlled trial that aims to evaluate such an approach. This paper describes the development of the implementation strategy for IMP2ART with particular focus on the integration of multiple level theories.

Methods

The Medical Research Council design and evaluation of complex interventions framework and the Person-Based Approach to intervention development were used as guidance for stages of strategy development. Specifically, we (i) set up a multidisciplinary team (including practicing and academic clinicians, health psychologists, public health and patient colleagues), (ii) reviewed and integrated evidence and theory, (iii) developed guiding principles, (iv) developed prototype materials, and (v) conducted a pre-pilot study before final refinement.

Results

The implementation strategy included resources for patients, team-based and individual healthcare professional education, practice audit and feedback, and an asthma review template, as well as a facilitator role accessible to primary care practices for 12 months. The synthesis of the integrated Promoting Action on Research Implementation in Health Services (iPARIHS) and Capability, Opportunity, Motivation and Behaviour (COM-B) frameworks led to an evolved framework bringing together important implementation and behaviour change elements which will be used as a basis for the study process evaluation.

Conclusions

A description of rigorous implementation strategy development for the IMP2ART study is provided along with newly theorised integration of implementation and behaviour change science which may be of benefit to others targeting implementation in primary care.

Trial registration

ISRCTN15448074. Registered on 2nd December 2019.
The effect of electricity consumption determinants in household load forecasting models
Authors: Bakiri Hussein A. , Mbembati Hadija
Published: November 2023

Usually, household electricity consumption fluctuates, often driven by several electrical consumption determinants such as income, household size, and price. Recently, research studies on the investigation of predictor variables in household electricity consumption have increased especially in the developing and newly industrialized countries. However, the studies just focus on identifying the predictor variables of household electricity consumption that influence load forecasting models. In Tanzania, for instance, scholars found that using the “income” determinant improves the performance of a forecasting model. The scholars suggest without any empirical bases that adding more predictor variables would have improved the accuracy of the model. This study aims to analyze the effect of the number of predictor variables on household load forecasting performance based on Tanzania’s data. Nonlinear regression based on a Weibull function and multivariate adaptive regression splines approaches are used for this purpose. Our findings indicate that income, household size, and number of appliances are common predictor variables of household consumption in developing countries. The measured forecasting root-mean-square error (RMSE) when using income, household size, and the number of appliances is 0.8244, 1.2314, and 0.9868, respectively. Finally, we forecasted load using all three determinants and the RMSE dropped to 0.7031. Having obtained the smaller value of RMSE when all predictors are used reveals that the inclusion of all three predictor variables in load forecasting leads to a significant decrease in RMSE by 14.73%. Therefore, the study recommends using multiple predictor variables in load forecasting models to increase accuracy.
Demography and fitness of Sesamia inferens Walker (Lepidoptera: Noctuidae) on three important gramineous crops
Authors: Li Chen , Shen Zhen , Tang Liang-De , Wang Xi-Ya , Huang Yuan-Qin , Zhang Yu-Fei , Mu Ming-Yue , Smagghe Guy , Zang Lian-Sheng
Published: November 2023

Background

Pink stem borer, Sesamia inferens (Walker), a major rice pest, has rarely been reported to cause serious damage to sorghum in China. During a survey of insect pests in glutinous sorghum for Chinese liquor production areas in Guizhou Province, it was found that S. inferens has caused serious damage to local sorghum in recent years.

Methods

In this context, we assessed the adaptation of S. inferens to three gramineous crop host plants (maize, rice and sorghum) based on age-stage, two-sex life table theory.

Results

Sesamia inferens had the highest development time, survival rate and total longevity on rice, followed by on sorghum and maize. There was no significant difference in intrinsic rate of increase and finite rate of increase among maize (r = 0.0702 day−1, λ = 1.0727 day−1), sorghum (r = 0.0681 day−1, λ = 1.0705 day−1) and rice (r = 0.0645 day−1, λ = 1.0666 day−1), but the mean generation period (T) was significantly higher for populations reared on rice (53.84 days) than on sorghum (49.53 days) and maize (47.31 days).

Conclusions

The results of our study indicate that S. inferens was able to complete the full developmental cycle on all three host plants. This study further supports that glutinous sorghum is an adaptable host plant for S. inferens.
Influence of pulse-tail energy of short-pulse CO2 laser in drilling of various glasses
Authors: Uno Kazuyuki , Kodama Yasushi , Yoneya Kazuyuki
Published: November 2023

In a short-pulse CO2 laser based on discharge excitation, there is a pulse tail that depends on the device configuration and operating conditions. The pulse tail is longer than the spike pulse and causes thermal effects such as a crack, heat-affected zones (HAZ), and so on. There are various types of glass having different physical constants related to heat, such as the thermal expansion coefficient and the softening point. Even if the same CO2 laser pulse is radiated onto glass, the processing results may differ depending on the glass material. Four types of glass, namely, crown glass, soda-lime glass, borosilicate glass, and synthetic quartz glass were irradiated with two types of short CO2 laser pulses, one with a large pulse tail and one with a small pulse tail, at a repetition rate of 200 Hz and a fluence per pulse of 22 J/cm2. As the processing characteristics, the ratios of the surface hole diameter and the HAZ diameter to the irradiation diameter, as well as the drilling depth, were investigated. The pulse-tail energy of the short CO2 laser pulses did not affect the surface hole diameter. In the glasses with small softening points of 740 °C or less, the pulse-tail energy of short CO2 laser pulses affected the HAZ with a large number of pulse irradiations with a total irradiation fluence of 2000 J/cm2 or more. The short CO2 laser pulses with a small tail produced a smaller HAZ than the short CO2 laser pulses with a large tail. In drilling with a large number of pulse irradiations, the short CO2 laser pulses with a small tail produced deeper drilling than the short CO2 laser pulses with a large tail. The glass material did not affect the surface hole diameter and the drilling depth. The glass material affected the HAZ.
Severe mental illness and the perimenopause
Authors: Behrman Sophie , Crockett Clair
Published: November 2023

Hormonal fluctuations in the perimenopause are associated with an array of physical and psychological symptoms. Those with pre-existing mental disorders may experience changes to their symptoms and response to treatment during the perimenopausal and postmenopausal periods and may also be at risk of poorer longer-term physical health outcomes in menopause. The transition towards menopause may be compounded by the oestradiol-suppressing effect of many psychotropics on the hypothalamopituitary–gonadal axis. A collaborative approach between primary care and secondary mental health services is an opportunity for proactive discussion of symptoms and support with management of the perimenopause. This may involve lifestyle measures and/or hormone replacement therapy, which can both lead to improvements in well-being and mental and physical health.