bluuebunny/crossref_metadata_2025 huggingface dataset:
Phosphorus-Induced Lipid Class Alteration Revealed by Lipidomic and Transcriptomic Profiling in Oleaginous Microalga Nannochloropsis sp. PJ12
Authors: Liang Jibei , Iqbal Sunya , Wen Fang , Tong Mengmeng , Liu Jianhua
Published: September 2019

Phytoplankton are primary producers in the marine ecosystem, where phosphorus is often a limiting factor of their growth. Hence, they have evolved strategies to recycle phosphorus by replacing membrane phospholipids with phosphorus-free lipids. However, mechanisms for replacement of lipid classes remain poorly understood. To improve our understanding, we performed the lipidomic and transcriptomic profiling analyses of an oleaginous marine microalga Nannochloropsis sp. PJ12 in response to phosphorus depletion (PD) and replenishing. In this study, by using (liquid chromatography couple with tandem mass spectrometry) LC-MS/MS-based lipidomic analysis, we show that membrane phospholipid levels are significantly reduced upon PD, while phosphorus-free betaine lipid levels are increased. However, levels of phosphorus-free photosynthetic galactolipid and sulfolipid are not increased upon PD, consistent with the reduced photosynthetic activity. RNA-seq-based transcriptomic analysis indicates that enzymes involved in phospholipid recycling and phosphorus-free lipid synthesis are upregulated, supporting the lipidomic analysis. Furthermore, enzymes involved in FASII (type II fatty acid synthesis) elongation cycle upon PD are transcriptionally downregulated. EPA (eicosapentaenoic acid) level decrease upon PD is revealed by both GC-MS (gas chromatography coupled with mass spectrometry) and LC-MS/MS-based lipidomic analyses. PD-induced alteration is reversed after phosphorus replenishing. Taken together, our results suggest that the alteration of lipid classes upon environmental change of phosphorus is a result of remodeling rather than de novo synthesis in Nannochloropsis sp. PJ12.
Biomarker Potential of Plasma MicroRNA-150-5p in Prostate Cancer
Authors: Paunescu Ionut Andrei , Bardan Razvan , Marcu Anca , Nitusca Diana , Dema Alis , Negru Serban , Balacescu Ovidiu , Balacescu Loredana , Cumpanas Alin , Sirbu Ioan Ovidiu , Petrut Bogdan , Seclaman Edward , Marian Catalin
Published: September 2019

Background and Objectives: Over decades, prostate cancer (PCa) has become one of the leading causes of cancer mortality in men. Extensive evidence exists that microRNAs (miRNAs or miRs) are key players in PCa and a new class of non-invasive cancer biomarkers. Materials and Methods: We performed miRNA profiling in plasma and tissues of PCa patients and attempted the validation of candidate individual miRs as biomarkers. Results: The comparison of tissue and plasma profiling results revealed five commonly dysregulated miRs, namely, miR-130a-3p, miR-145-5p, miR-148a-3p, miR-150-5p, and miR-365a-3p, of which only three show concordant changes—miR-130a-3p and miR-150-5p were downregulated and miR-148a-3p was upregulated in both tissue and plasma samples, respectively. MiR-150-5p was validated as significantly downregulated in both plasma and tissue cancer samples, with a fold change of −2.697 (p < 0.001), and −1.693 (p = 0.035), respectively. ROC analysis showed an area under the curve (AUC) of 0.817 (95% CI: 0.680–0.995) for plasma samples and 0.809 (95% CI: 0.616–1.001) for tissue samples. Conclusions: We provide data indicating that miR-150-5p plasma variations in PCa patients are associated with concordant changes in prostate cancer tissues; however, given the heterogeneous nature of previous findings of miR-150-5p expression in PCa cells, additional future studies of a larger sample size are warranted in order to confirm the biomarker potential and role of miRNA-150-5p in PCa biology.
Lead-Related Infective Endocarditis in Latvia: A Single Centre Experience
Authors: Nesterovics Nikolajs , Nesterovics Georgijs , Stradins Peteris , Kalejs Martins , Ansabergs Janis , Blumbergs Maris , Maca Aija , Kamzola Ginta , Lejnieks Aivars , Kalejs Oskars , Erglis Andrejs
Published: September 2019

Background and Objectives: Over the last five decades cardiac implantable electronic devices (CIED) have become established as the mainstay for the treatment of permanent bradycardias, chronic heart failure and dangerous heart rhythm disturbances. These devices improve survival and quality of life in many patients. However, infections associated with CIED implantation, particularly lead-related infective endocarditis (LRIE), can offset all benefits and make more harm than good for the patient. To date, there are no other studies in Latvia, addressing patients with lead-related infective endocarditis. The objective of this study was to identify the most common pathogens associated with LRIE and their antimicrobial resistance and to identify possible risk factors of patients who present with LRIE. Materials and Methods: The study was performed retrospectively at Pauls Stradins Clinical University Hospital (PSCUH). The study included patients who were referred to PSCUH due to LRIE for lead extraction. Patients were identified from procedural journals. Information about isolated microorganisms, patient comorbidities and visual diagnostics data was taken from patient records. Results: Forty-nine patients with CIED related infective endocarditis were included in the study, 34 (69.4%) were male, median age of all patients was 65.0 (50.5–73.0) years, median hospital stay was 15.5 (22.0–30.5) days. Successful and complete lead extraction was achieved in all patients. Thirty-two (65.3%) had received antibiotics prior to blood sample. Only in 31 (63.3%) positive culture results were seen. The most common isolated pathogens were Staphylococcus aureus (23.5%) and coagulase negative staphylococci (23.5%). Other bacteria were isolated considerably less often. The atrial lead was most common location for lead vegetations, seen in 50.0% of cases. Five (10.2%) patients have died due to the disease. Conclusions: Lead-related infective endocarditis is a major complication of cardiac implantable electronic devices with considerable morbidity and mortality, which in our study was as high as 10.2%.
Early Stage Glycosylation Biomarkers in Alzheimer’s Disease
Authors: Regan Patricia , McClean Paula L. , Smyth Thomas , Doherty Margaret
Published: September 2019

Alzheimer’s disease (AD) is of great cause for concern in our ageing population, which currently lacks diagnostic tools to permit accurate and timely diagnosis for affected individuals. The development of such tools could enable therapeutic interventions earlier in the disease course and thus potentially reducing the debilitating effects of AD. Glycosylation is a common, and important, post translational modification of proteins implicated in a host of disease states resulting in a complex array of glycans being incorporated into biomolecules. Recent investigations of glycan profiles, in a wide range of conditions, has been made possible due to technological advances in the field enabling accurate glycoanalyses. Amyloid beta (Aβ) peptides, tau protein, and other important proteins involved in AD pathogenesis, have altered glycosylation profiles. Crucially, these abnormalities present early in the disease state, are present in the peripheral blood, and help to distinguish AD from other dementias. This review describes the aberrant glycome in AD, focusing on proteins implicated in development and progression, and elucidates the potential of glycome aberrations as early stage biomarkers of AD.
The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin
Authors: Ruiz Pamela , Balado Miguel , Fuentes-Monteverde Juan Carlos , Toranzo Alicia E. , Rodríguez Jaime , Jiménez Carlos , Avendaño-Herrera Ruben , Lemos Manuel L.
Published: September 2019

Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals.
Sixteen New Prenylated Flavonoids from the Fruit of Sinopodophyllum hexandrum
Authors: Sun Yanjun , Chen Haojie , Wang Junmin , Gao Meiling , Zhao Chen , Han Ruijie , Chen Hui , Li Meng , Xue Guimin , Feng Weisheng
Published: September 2019

Sixteen new prenylated flavonoids, sinoflavonoids P–Z (1–11) and sinoflavonoids NA–NE (12–16), were isolated from the fruit of Sinopodophyllum hexandrum, along with eight known analogues (17–24). Their structures were elucidated on the basis of extensive spectroscopic data (HR-ESI-MS, 1H-NMR, 13C-NMR, HSQC, HMBC). The cytotoxic activities of compounds 1–18, 20, and 22 were evaluated by MTT assay. Compound 6 showed the most potent cytotoxicity in MCF-7, and HepG2 cell lines, with IC50 values of 6.25 and 3.83 μM, respectively.
Evaluation of an Eight-Week Whole-Food Plant-Based Lifestyle Modification Program
Authors: Campbell Erin K. , Fidahusain Mohammad , Campbell II Thomas M.
Published: September 2019

Poor diet quality is the leading cause of death both in the United States and worldwide, and the prevalence of obesity is at an all-time high and is projected to significantly worsen. Results from an eight-week group program utilizing an ad-libitum whole-food plant-based dietary pattern, were reviewed. There were 79 participants, all self-referred from the community, including 24 (30.4%) who were already vegetarian or vegan at baseline. Seventy-eight participants (98.7%) completed the eight-week program. Among completers, those with higher BMI at baseline lost a larger percentage of their body weight (total body weight loss of 3.0 ± SD 2.1%, 5.8 ± 2.8%, and 6.4 ± 2.5% for participants who had baseline BMI in normal, overweight, and obese range, respectively). The average weight loss for all the completers was 5.5 ± 3.0 kg (p < 0.0001). Final blood pressure and plasma lipids were reduced compared to baseline (SBP decreased 7.1 ± 15.5 mmHg (p = 0.0002), DBP decreased 7.3 ± 10.9 mmHg (p < 0.0001), total cholesterol decreased 25.2 ± 24.7 mg/dL (p < 0.0001), LDL decreased 15.3 ± 21.1 mg/dL (p < 0.0001)). Twenty-one (26.9%) participants were able to decrease or stop at least one chronic medication compared to two (2.6%) participants who required an increased dose of a chronic medication. Participants who were already vegetarian or vegan at baseline experienced statistically significant weight loss and reductions in total and LDL cholesterol. There was a non-significant trend toward less weight loss in these participants compared to participants who were non-vegetarian at baseline. Reductions in total and LDL cholesterol were not significantly different when comparing vegetarian or vegan and non-vegetarian participants. A whole-food plant-based dietary intervention may provide significant short-term benefits for both non-vegetarian, vegetarian, and vegan individuals.
Urolithin Metabotypes can Anticipate the Different Restoration of the Gut Microbiota and Anthropometric Profiles during the First Year Postpartum
Authors: Cortés-Martín Adrián , Romo-Vaquero María , García-Mantrana Izaskun , Rodríguez-Varela Ana , Collado María Carmen , Espín Juan Carlos , Selma María Victoria
Published: September 2019

The metabolism of dietary polyphenols ellagitannins by the gut-microbiota allows the human stratification in urolithin metabotypes depending on the final urolithins produced. Metabotype-A only produces urolithin-A, metabotype-B yields urolithin-B and isourolithin-A in addition to urolithin-A, and metabotype 0 does not produce urolithins. Metabotype-A has been suggested to be ‘protective’, and metabotype-B dysbiotic-prone to cardiometabolic impairments. We analyzed the gut-microbiome of 40 healthy women and determined their metabotypes and enterotypes, and their associations with anthropometric and gut-microbial changes after 3 weeks, 4, 6, and 12 months postpartum. Metabotype-A was predominant in mothers who lost weight (≥2 kg) (75%) versus metabotype-B (54%). After delivery, the microbiota of metabotype-A mothers changed, unlike metabotype-B, which barely changed over 1 year. The metabotype-A discriminating bacteria correlated to the decrease of the women’s waist while some metabotype-B bacteria were inversely associated with a reduction of body mass index (BMI), waist, and waist-to-hip ratio. Metabotype-B was associated with a more robust and less modulating microbial and anthropometric profiles versus metabotype-A, in which these profiles were normalized through the 1-year follow-up postpartum. Consequently, urolithin metabotypes assessment could be a tool to anticipate the predisposition of women to normalize their anthropometric values and gut-microbiota, significantly altered during pregnancy and after childbirth.
Topic Analysis of UK Fitness to Practise Cases: What Lessons Can Be Learnt?
Authors: Hanna Alan , Hanna Lezley-Anne
Published: September 2019

Background: Fitness to practise (FtP) impairment (failure of a healthcare professional to demonstrate skills, knowledge, character and/or health required for their job) can compromise patient safety, the profession’s reputation, and an individual’s career. In the United Kingdom (UK), various healthcare professionals’ FtP cases (documents about the panel hearing(s) and outcome(s) relating to the alleged FtP impairment) are publicly available, yet reviewing these to learn lessons may be time-consuming given the number of cases across the professions and amount of text in each. We aimed to demonstrate how machine learning facilitated the examination of such cases (at uni- and multi-professional level), involving UK dental, medical, nursing and pharmacy professionals. Methods: Cases dating from August 2017 to June 2019 were downloaded (577 dental, 481 medical, 2199 nursing and 63 pharmacy) and converted to text files. A topic analysis method (non-negative matrix factorization; machine learning) was employed for data analysis. Results: Identified topics were criminal offences; dishonesty (fraud and theft); drug possession/supply; English language; indemnity insurance; patient care (including incompetence) and personal behavior (aggression, sexual conduct and substance misuse). The most frequently identified topic for dental, medical and nursing professions was patient care whereas for pharmacy, it was criminal offences. Conclusions: While commonalities exist, each has different priorities which professional and educational organizations should strive to address.
Roles of Hardened Husks and Membranes Surrounding Brachypodium hybridum Grains on Germination and Seedling Growth
Authors: El-Keblawy Ali , Elgabra Masarra , Mosa Kareem A. , Fakhry Amal , Soliman Sameh
Published: September 2019

Several studies have assessed the function and significance of the presence of dead, hardened husks on germination and seedling growth in several grass species and reached to inconsistent results. Here, we assess the roles of husks (dead lemma and palea) and an inner membrane surrounding the grains on germination behaviour and seedling growth of Brachypodium hybridum, one of three species of the genetic model B. distachyon complex, in an arid mountain of Arabia. The interactive effects between temperature and the incubation light were assessed on germination of husked and dehusked-demembraned grains. Germination and seedling growth were assessed for different combinations of grain treatments (soaked and non-soaked husked, dehusked-membraned and dehusked-demembraned). Dehusked-demembraned grains were also germinated in different dormancy regulating compounds (DRCs) and light qualities (light, dark and different red: far red [R: FR] ratios). The results indicated an insignificant difference between husked and dehusked-membraned grains on final germination and the germination rate index (GRI), with the former producing significantly bigger seedlings. Removal of the inner-membrane resulted in a significant reduction in all traits. Soaking grains in water resulted in significant enhancements in germination and seedling growth of only husked grains. Husked-membraned and demembraned grains germinated more significantly and faster at lower rather than higher temperatures. None of different concentrations of several DRCs succeeded in enhancing final germination of dehusked-demembraned grains. Red-rich light significantly enhanced germination of dehusked-membraned grains in comparison to other light qualities. It could be concluded that the role of husks is to mainly enhance seedling growth, while the major role of the membrane is to increase final germination. The ability of red-rich light in enhancing the germination of dehusked-membraned but not dehusked-demembraned grains suggest a role for the inner membrane in regulating dormancy through differential filtering of light properties.
Genetically Modified Heat Shock Protein90s and Polyamine Oxidases in Arabidopsis Reveal Their Interaction under Heat Stress Affecting Polyamine Acetylation, Oxidation and Homeostasis of Reactive Oxygen Species
Authors: Toumi Imene , Pagoulatou Marianthi G. , Margaritopoulou Theoni , Milioni Dimitra , Roubelakis-Angelakis Kalliopi A.
Published: September 2019

The chaperones, heat shock proteins (HSPs), stabilize proteins to minimize proteotoxic stress, especially during heat stress (HS) and polyamine (PA) oxidases (PAOs) participate in the modulation of the cellular homeostasis of PAs and reactive oxygen species (ROS). An interesting interaction of HSP90s and PAOs was revealed in Arabidopsis thaliana by using the pLFY:HSP90RNAi line against the four AtHSP90 genes encoding cytosolic proteins, the T-DNA Athsp90-1 and Athsp90-4 insertional mutants, the Atpao3 mutant and pharmacological inhibitors of HSP90s and PAOs. Silencing of all cytosolic HSP90 genes resulted in several-fold higher levels of soluble spermidine (S-Spd), acetylated Spd (N8-acetyl-Spd) and acetylated spermine (N1-acetyl-Spm) in the transgenic Arabidopsis thaliana leaves. Heat shock induced increase of soluble-PAs (S-PAs) and soluble hydrolyzed-PAs (SH-PAs), especially of SH-Spm, and more importantly of acetylated Spd and Spm. The silencing of HSP90 genes or pharmacological inhibition of the HSP90 proteins by the specific inhibitor radicicol, under HS stimulatory conditions, resulted in a further increase of PA titers, N8-acetyl-Spd and N1-acetyl-Spm, and also stimulated the expression of PAO genes. The increased PA titers and PAO enzymatic activity resulted in a profound increase of PAO-derived hydrogen peroxide (H2O2) levels, which was terminated by the addition of the PAO-specific inhibitor guazatine. Interestingly, the loss-of-function Atpao3 mutant exhibited increased mRNA levels of selected AtHSP90 genes. Taken together, the results herein reveal a novel function of HSP90 and suggest that HSP90s and PAOs cross-talk to orchestrate PA acetylation, oxidation, and PA/H2O2 homeostasis.
Harnessing the Topography of 3D Spongy-Like Electrospun Bundled Fibrous Scaffold via a Sharply Inclined Array Collector
Authors: Cho Sun Hee , Kim Jeong In , Kim Cheol Sang , Park Chan Hee , Kim In Gi
Published: September 2019

To date, many researchers have studied a considerable number of three-dimensional (3D) cotton-like electrospun scaffolds for tissue engineering, including the generation of bone, cartilage, and skin tissue. Although numerous 3D electrospun fibrous matrixes have been successfully developed, additional research is needed to produce 3D patterned and sophisticated structures. The development of 3D fibrous matrixes with patterned and sophisticated structures (FM-PSS) capable of mimicking the extracellular matrix (ECM) is important for advancing tissue engineering. Because modulating nano to microscale features of the 3D fibrous scaffold to control the ambient microenvironment of target tissue cells can play a pivotal role in inducing tissue morphogenesis after transplantation in a living system. To achieve this objective, the 3D FM-PSSs were successfully generated by the electrospinning using a directional change of the sharply inclined array collector. The 3D FM-PSSs overcome the current limitations of conventional electrospun cotton-type 3D matrixes of random fibers.
Fault Diagnosis Method for Hydraulic Directional Valves Integrating PCA and XGBoost
Authors: Lei Yafei , Jiang Wanlu , Jiang Anqi , Zhu Yong , Niu Hongjie , Zhang Sheng
Published: September 2019

A novel fault diagnosis method is proposed, depending on a cloud service, for the typical faults in the hydraulic directional valve. The method, based on the Machine Learning Service (MLS) HUAWEI CLOUD, achieves accurate diagnosis of hydraulic valve faults by combining both the advantages of Principal Component Analysis (PCA) in dimensionality reduction and the eXtreme Gradient Boosting (XGBoost) algorithm. First, to obtain the principal component feature set of the pressure signal, PCA was utilized to reduce the dimension of the measured inlet and outlet pressure signals of the hydraulic directional valve. Second, a machine learning sample was constructed by replacing the original fault set with the principal component feature set. Third, the MLS was employed to create an XGBoost model to diagnose valve faults. Lastly, based on model evaluation indicators such as precision, the recall rate, and the F1 score, a test set was used to compare the XGBoost model with the Classification And Regression Trees (CART) model and the Random Forests (RFs) model, respectively. The research results indicate that the proposed method can effectively identify valve faults in the hydraulic directional valve and have higher fault diagnosis accuracy.
Synthetic Effect of EDTA and Ni2+ on Methane Production and Microbial Communities in Anaerobic Digestion Process of Kitchen Wastes
Authors: Zhong Tingting , Liu Yali , Kang Xiaorong
Published: September 2019

Batch tests were carried out to study the effect of simultaneous addition of ethylenediaminetetraacetic acid and Ni2+ (EDTA-Ni) on anaerobic digestion (AD) performances of kitchen wastes (KWs). The results indicated that the cumulative biogas yield and methane content were enhanced to 563.82 mL/gVS and 63.7% by adding EDTA-Ni, respectively, which were almost 1.15 and 1.07-fold of that in the R2 with Ni2+ addition alone. At the same time, an obvious decrease of propionic acid was observed after EDTA-Ni addition. The speciation analysis of Ni showed that the percentages of water-soluble and exchangeable Ni were increased to 38.8% and 36.3% due to EDTA-Ni addition, respectively. Also, the high-throughput sequencing analysis revealed that the EDTA-Ni promoted the growth and metabolism of Methanosarcina and Methanobacterium, which might be the major reason for propionic acid degradation and methane production.
Characterization of Simple and Double Yeast Cells Using Dielectrophoretic Force Measurement
Authors: García-Diego Fernando-Juan , Rubio-Chavarría Mario , Beltrán Pedro , Espinós Francisco J.
Published: September 2019

Dielectrophoretic force is an electric force experienced by particles subjected to non-uniform electric fields. In recent years, plenty of dielectrophoretic force (DEP) applications have been developed. Most of these works have been centered on particle positioning and manipulation. DEP particle characterization has been left in the background. Likewise, these characterizations have studied the electric properties of particles from a qualitative point of view. This article focuses on the quantitative measurement of cells’ dielectric force, specifically yeast cells. The measures are obtained as the results of a theoretical model and an instrumental method, both of which are developed and described in the present article, based on a dielectrophoretic chamber made of two V-shaped placed electrodes. In this study, 845 cells were measured. For each one, six speeds were taken at different points in its trajectory. Furthermore, the chamber design is repeatable, and this was the first time that measurements of dielectrophoretic force and cell velocity for double yeast cells were accomplished. To validate the results obtained in the present research, the results have been compared with the dielectric properties of yeast cells collected in the pre-existing literature.
Sequent Occupance and Toponymy in Singapore: The Diachronic and Synchronic Development of Urban Place Names
Authors: Cavallaro Francesco , Perono Cacciafoco Francesco , Tan Zhi Xuan
Published: September 2019

This paper is aimed at investigating the applicability of the notion of Sequent Occupance to the Singapore context. Sequent Occupance as a phenomenon in Human Geography was first theorized by Derwent Whittlesey in 1929 in order to describe the current cultural landscape of a region as a combination of all the people which have ‘sequentially’ occupied that region from the past to the present. According to the Sequence Occupance Theory, the cultural imprint of each civilization is never completely lost and its traces can be seen to the present day. This is a historical phenomenon that occurs in the same region or space, but at different times. Sequent Occupance regards each region as a pattern of many cultural layers laid upon each other, where each layer can be attributed to a particular civilization or culture, which overlaps the ones before it. Singapore, with its multilingual and multicultural context and with its colonial past, is a very important test-bed for Sequence Occupance approaches both in the fields of Historical Toponomastics and Human Geography. This paper aims to apply the notion of Sequence Occupance to the study of Singapore Toponomastics with a focus on Odonymy and Micro-Toponyms. The study discusses the notion of Sequent Occupance in Singapore in the light of several local Toponyms, trying to ascertain if this concept can be applied to the diachronic and synchronic development of the Urban Toponymy of the Lion City. The article also highlights historical processes in the “making” of the multi-layered Singapore society.
The Impact of Education and Feedback on the Accuracy of Pressure Injury Staging and Documentation by Bedside Nurses
Authors: Sankovich Kathleen , Fenimore Laura Ann , Hoffman Rose , Ren Dianxu
Published: September 2019

Background: Pressure Injuries (PIs) are largely preventable. Accurate documentation of PI stage or progression is a key quality measure.

Local Problem: Nurses frequently fail to accurately assess and document their findings in the electronic medical record. This project sought to increase nurses’ knowledge and accuracy of staging and documentation of PIs.

Method: Educational interventions; direct observation of PI status; review of nurse documentation; feedback,;and referrals to wound, ostomy, and continence nurses (WOCNs).

Interventions: Nurses completed a pre- and post-test and online training modules, and participated in training sessions. Clinical experts completed direct skin observations and provided feedback about PI staging.

Results: There was a statistically significant improvement in nurses’ knowledge about PIs (p = 0.004). Skin assessments were conducted on 108 patients (13 PIs identified). The bedside nurse accurately assessed a PI stage in only 31% of these observations. Referrals to WOCNs increased by 18% compared to the baseline period.

Conclusions: Educational interventions enhanced nurses’ knowledge; however, appropriate PI staging may require skills development and validation to build competency.

Keywords: pressure injury, pressureulcer,wound care, prevention, evidence-based practice, prevalence, assessment, documentation, education
Allergy-Related Medication Error Reports Submitted to a Large Patient Safety Reporting System
Authors: Grissinger Matthew , Gaunt Michael , Shilman Alexander
Published: September 2019

Medication allergies can and do cause patient harm. Managing a patient’s allergies is a challenge for institutionsbecause failures can happen throughout the medication-use process. A total of 854 Medication Error events associated with patient allergies that occurred between July 2016 and June 2018 were reported through a large event reporting database.

Analysts categorized these events into the following five stages: obtaining information from the patient, documenting allergies in the record, ordering medications, verifying orders, and administering medications. More than half (56.3%; n = 481) of the events reached the patient. Most likely to reach patients were events involving breakdowns when obtaining information from the patient (74.7%, n = 68 of 91) and administering medications (97.6%, n = 281 of 288). In reports that indicated allergies were properly documented, the majority (87.3%, n = 289 of 331) of the events that reached patients passed through two or more stages. Organizations may use this information to inform proactive efforts to implement system-based strategies to improve the medication-use process.

Keywords: drug allergy, drug reaction, medication errors, medication safety, patient safety
Results of the Patient Safety Authority's 2018 Process Measures Survey
Authors: Bell Michelle , Liberatore Kim , Finley Edward
Published: September 2019

Improving patient safety is an ongoing journey that benefits from periodic assessment to recognize, reward, and redirect efforts. As an independent state agency, the Patient Safety Authority (PSA) is uniquely positioned to both conduct comprehensive safety assessments and support improvement efforts. A process measures survey of acute care facilities was conducted in November and December 2018. The purpose was to inform the PSA’s strategic direction, provide benchmarking data to facilities, and understand the current patient safety landscape. The survey consisted of 48 questions divided into 10 domains: Behavioral Health, Falls, Health Information Technology, Improving Diagnosis, Infection Prevention and Control, Leadership, Medication Safety, Obstetrics, Safe Surgery, and Transition of Care. Each question asked respondents to report the degree to which a specific safety practice has been implemented at their facility.

In all, 153 unique facility responses with at least 30% of the survey questions completed were received and analyzed. According to respondents, the domains Safe Surgery, Infection Prevention and Control, and Obstetrics had the highest percentages of full implementation, while Behavioral Health, Medication Safety, and Improving Diagnosis had the lowest. Looking across domains, two new themes emerged: first, a high percentage of full implementation of safety practices to support communication about patient safety with frontline staff and second, a low percentage of full implementation of safety practices that promote patient engagement in organizational efforts to support safe patient care. These results will inform the PSA’s focus over the next several years.
Antropologia e literatura: diálogos sobre experiências psicoativas
Authors: Boarin Fernanda Vivacqua de Souza Galvão , Filho Paulo César Caminha Ramos
Published: September 2019

Este artigo é uma tentativa de diálogo entre a antropologia e a literatura na temática dos psicoativos. Por intermédio da conexão dos autores com as substâncias psicoativas, almejamos perceber, inicialmente, que a questão da experiência é importante para a construção desse tipo de conhecimento. Portanto, com a imersão da experiência na escrita desses autores, pretendemos demonstrar a alteração da consciência sendo explorada através de relatos em ambas as disciplinas. Desse modo, o objetivo é trazer à tona tais relatos para que possamos perceber que os diferentes usos dos diversos psicoativos constroem esse mosaico da experiência como plural e calcado na experiência da linguagem.
A tutela dos corpos pelo Estado e a produção do corpo sem órgãos: como ganhar terreno a partir dos direitos da personalidade
Authors: Zanon Caio Hoffmann Cardoso
Published: September 2019

O objetivo deste artigo é o questionamento da atual regulamentação dos direitos da personalidade pelo Código Civil de 2002 à luz da teoria de Deleuze e Guattari sobre o corpo sem órgãos. Como método, utiliza-se de uma análise primeiro da lei e, em seguida, da principal proposta que se tem nos dias atuais para uma reinterpretação do regime dos direitos da personalidade, que seria a doutrina Civil-constitucional, a partir do marco teórico proposto. Como conclusão, chega-se à afirmação de que tanto a regulamentação do Código Civil como a proposta Civil-constitucional não têm êxito em acolher de forma plena a multiplicidade, pois permanecem ligadas à ideia de indivíduo em vez de a uma visão que privilegie os fluxos e intensidades que passam pelos corpos. Propõem-se pesquisas futuras no que diz respeito a formas de desestratificação dos corpos e à identificação de deformações anárquicas no texto constitucional que possibilitem uma realidade acolhedora da diversidade.
“PALHAÇOS” CERIMONIAIS JÊ
Authors: Dias Lucília da Glória Alves
Published: September 2019

Procurando realizar uma discussão sobre o lugar do humor no estabelecimento de vínculos com o exterior do socius, apresento nesse trabalho uma leitura dos dados sobre palhaços cerimoniais que estão presentes em etnografias sobre povos jê produzidas entre as décadas de 1970 e 2010. Veremos que, ao abordar em suas apresentações temas como comida, sexo e relação com seres que habitam o exterior da aldeia, esses personagens cômicos estão performatizando um pensamento Jê sobre o parentesco. Ao que parece, os palhaços jê em seu comportamento grotesco, teriam como espelho as condutas consideradas socialmente adequadas. As atitudes grotescas desses personagens cômicos não se opõem à ordem social, mas se desenvolvem em diálogo com ela, isto é, produzindo uma imaginação para além ou aquém da ordem.
ESTÉTICA E SOCIABILIDADE: O SALÃO DE BELEZA COMO PONTO DE ENCONTRO
Authors: Ferreira Daniela Alexandre
Published: September 2019

O seguinte artigo visa entender como são pautadas as relações sociais em um salão de beleza situado na cidade de Santos Dumont. Como espaço de sociabilidade feminina, procura identificar sua importância para a vida social de suas freqüentadoras. Através de uma investigação qualitativa, baseada na observação e realização de entrevistas semi-estruturadas. Observa-se que além de se configurar como centro de estética, atua como ponto de encontro de uma rede de mulheres que frequentam a vizinhança e de seus filhos, podendo usufruir desse ambiente, diversos usos sociais estimulando a vivência da rua e suas calçadas como espaço de lazer, na vida cotidiana de uma cidade do interior de Minas Gerais.
Estado e capital fictício: o Novo Regime Fiscal no Brasil
Authors: Vazquez João Pedro Pereira
Published: September 2019

Esse trabalho objetiva dissertar sobre a relação entre Estado e capital fictício no Brasil, realizando uma recapitulação histórica da financeirização do capital, processo em que Estado e capital fictício estiveram envolvidos organicamente, e identificando os impactos sobre a América Latina, em geral, e sobre o Brasil, em particular, em que se parte de um exame da dívida pública enquanto forma concreta do capital fictício. Ademais, apoiado numa sumária investigação da conjuntura brasileira recente, realiza-se uma análise do Novo Regime Fiscal desde o prisma da relação Estado-capital fictício.
REDEMOCRATIZAÇÃO E DIREITO À REPARAÇÃO: ANÁLISE SOBRE AS COMISSÕES NACIONAIS DA VERDADE NO BRASIL E NA ARGENTINA
Authors: Esteves Carlos Alberto , Boarin Paula
Published: September 2019

Este artigo propõe quatro possibilidades de análise para a Comissão Nacional da Verdade, no Brasil, e para a Comisión Nacional sobre la Desaparición de Personas, na Argentina. Tendo em vista que esses países adotaram diferentes caminhos para a conformação do direito à reparação relativa às violações aos direitos humanos ao longo do referido período, pergunta-se quais abordagens podem fornecer elementos válidos para sua compreensão. Deste modo, intenciona-se explorar, em perspectiva comparada, as seguintes dimensões de análise: jurídico-transicional e institucional e participativa.
Noção de Pessoa e Subjetividade: a intimidade e suas transformações sob a ótica de Anthony Giddens
Authors: Dos Santos Bruna Motta , De Oliveira Bruno Hermes de Oliveira Hermes
Published: September 2019

O mundo contemporâneo é palco de profundas e aceleradas transformações, cujos impactos têm repercutido nas dimensões mais íntimas da vida dos sujeitos. O sociólogo Anthony Giddens é um dos autores que tem se preocupado com a questão, buscando compreender as consequências da modernidade e seus entrelaçamentos com a vida pessoal e com o Eu (self). Nosso objetivo foi analisar a discussão de Giddens acerca dos impactos do mundo contemporâneo sobre a intimidade, bem como tecer uma breve análise das implicações dessas transformações. A investigação foi conduzida a partir da análise de duas obras do referido autor: “As consequências da modernidade" e “Transformação da Intimidade: sexualidade, amor e erotismo nas sociedades modernas”. Para o autor, o fenômeno sociológico da “transformação da intimidade” caracteriza-se pela modificação na forma como as relações amorosas se estruturam, estando cada vez mais motivadas e sustentadas por aspectos democráticos, como a autonomia do Eu e do Outro. Frente às alterações nas condutas de vida dos sujeitos e suas relações com a dimensão da intimidade sugeridas por Giddens, podemos entrever um adensamento da noção de pessoa enquanto indivíduo nos dias atuais.
Aportaciones históricas y arqueológicas al estudio del proceso de estandarización en la industria ancorera de las potencias marítimas europeas del siglo XVIII
Authors: Ciarlo Nicolás C.
Published: September 2019

Las anclas fueron un elemento de capital importancia a lo largo de la historia de la navegación. En el siglo XVIII, los barcos de guerra europeos utilizaban varios tipos y dependían de ellas para fondear y realizar otras maniobras. La industria ancorera constituyó en aquel entonces una de las expresiones más complejas de la tecnología de forjado del hierro y, en el marco de un proceso de industrialización creciente, estuvo sujeta a varios cambios. En este artículo examinamos las características de las anclas, en materia de diseño y manufactura, de las principales potencias marítimas. A la luz de las fuentes documentales y materiales disponibles, discutimos aspectos relativos al proceso de estandarización y cambio tecnológico en la industria ancorera. Complementariamente, presentamos algunos lineamientos para el estudio de anclas de procedencia arqueológica.
¿Adobes, terrones o bolas de barro amasado? Aportaciones para el reconocimiento arqueológico de las distintas técnicas constructivas que emplean módulos de tierra
Authors: Pastor Quiles María , Knoll Franziska , Jover Maestre Francisco J.
Published: September 2019

En contextos arqueológicos es habitual la documentación de estructuras inmuebles en las que se emplea la tierra como material constructivo. Entre las técnicas constructivas con tierra que emplean elementos modulares se encuentran el adobe, el amasado de barro y el terrón. No obstante, a pesar de ser técnicas reconocidas y haber sido empleadas hasta la actualidad, en los estudios arqueológicos se encuentran importantes dificultades para su reconocimiento. La importancia de su correcta identificación reside, sobre todo, en las implicaciones económicas y sociales que se derivan de su aplicación y desarrollo. Con este trabajo abordamos la caracterización de las distintas técnicas señaladas, con el objetivo de contribuir a una mejor diferenciación entre ellas. A través de su descripción y análisis, atendiendo principalmente a sus características macroscópicas, y de la presentación de un caso de estudio específico de la Edad del Bronce de la península ibérica, se aporta una serie de consideraciones orientadas a concretar qué criterios pueden permitir un mejor reconocimiento de estas técnicas, atendiendo especialmente a su estudio como unidades individualizadas.
Estimating Genetic Parameters using a Random Regression Model
Authors: Márkus Szilárd , Němcová Eva , Fazekas István , Komlósi István
Published: September 2019

One of the most important part of the genetic evaluation using a random regression model is the estimation of variance components. This is the topic of many papers because the large computational costs. We can use restricted maximum likelihood (REML), Gibbs sampling and ℜ method for the estimation of genetic parameters. The variance components are necessary to calculate the heritabilities and repeatabilities.The aim of our paper is to estimate the variance components using a random regression repeatability model from test day data set of Hungarian Holstein-Friesian dairy cows and to analyse the change of additive genetic and permanent environmental variance, heritability and repeatability over lactation.
The evaluation of body condition scoring of Holstein-Friesian cows
Authors: Mikó Józsefné Jónás Edit , Mucsi Imre , Komlósi István
Published: September 2019

We carried out the examinations on a farm of 500 cows where We examined the body condition of the stock every month. We recorded the body condition data together with the milk production and calving data. We analysed the daily milk production as well as the body condition. During the examinations we sought an answer how the body condition and the production level change during the lactation period.
Production and disposition of purebred Jersey populations
Authors: Szendrei Zoltán , Béri Béla
Published: September 2019

In Hungary crossbreeding wit Jersey has been in practice since 2004. Uniquely in the Hungarian history of the Jersey breed two times fifty head pregnant heifers were imported in 2006. The aim of our study is to present through the description of the production and the type traits of the population the inherent possibilities of Jersey. The breed is presented by the figures of the rolling lactations, 305 day milk production, correlations between the traits and by the results of the evaluation of type traits. Analyses of the data were done by using independent samples t-test.Based on data of 87 cows the average number of days in milk  is 308 and 48 cows has finished lactation. During the rolling lactation they produced 5050 kg milk with 5.34% fat and 3.8% protein content.The first lactation Jersey cows’ production when corrected to 305 days was 5089 kg milk with 5.35% fat and 3.81% protein. Between the herds there was significant difference in milk kg, amount of fat produced, protein content and amount of protein produced. On the two farms distribution of cows belonging to different production levels is very different. The biggest difference is when categorized by the amount of milk produced.Correlation between the milk kg and fat kg is the greatest (r=0.950). Similar values were obtained for the correlation between fat and protein kg (r=0.919) and for the milk kg and fat kg (r=0,898). Disposition of the Jersey cows is very well balanced. Their stature, strength, body depth, dairy form, rump angle and pin width is favorable, the average scores are between 4.5 and 6.5. The leg of the cows is a bit sickly, and is hocking in a little with medium-low angled hoof. Average values of all traits describing the udder are between 4 and 5. Cows belonging to the two herds differ by disposition. Significant difference exists in the following traits: body depth, dairy form, pin width, rear leg - side view, rear leg, rear view, foot angle, fore udder attachment, teat length. TheJersey population imported to Hungary has a final score of 78 points which equals with the “good” qualification.
PENGARUH MEMPELAJARI BAHASA ASING TERHADAP PERKEMBANGAN OTAK ANAK
Authors: HAKIM FURQANUL , CHIANI SARASWATI HAYLIAN
Published: September 2019

Children's intelligence shows how we stimulate their brains. At present, all parents are actively looking for the best stimulus for their children's brain growth. But most parents still use traditional methods in stimulating the brains of their children. For example, parents usually use the instant method by giving their children some brain enhancing drugs. Some experts believe that this method is not an effective method to be used.   This study aims to determine the correlation of learning foreign languages with the growth of children's brains, and to find out the benefits of learning foreign languages for the growth of children's brains   This research is conducted at a course called 24 English College Bima NTB, with direct observation methods and conducted interviews based on questionnaires to respondents who are determined by techinique random sampling, then the results of the study are analyzed qualitatively and supported by references (books, printed media or electronically related to the problem).  The results of the study show that: (1). By learning foreign languages such as English, Spanish, French, etc. Can stimulate children's brain growth effectively, and intelligence on children also depends on what type of stimulus they get. (2). The benefits of mastering a foreign language are (a) it maximizes children's brain performance, (b) Children with good foreign language skills have high intelligence and creativity, (c) can decrease senile dimensions, (d). have better cognitive flexibility, (e). delay Alzheimer's disease, (f). Children are able to solve problems if they use a foreign language, (g). Good decision makers. People who think in other languages tend to make rational decisions
DISTANCE AND DOSAGE OF ORGANIC FERTILIZER INFLUENCE RESULTS OF EGG CABBAGE PLANT (BRASSICA OLERACEAE VAR. CAPITATA L)
Authors: WINTEN KETUT TURAINI INDRA , GUNAMANTA PANDE GEDE , PUTRA ANAK AGUNG GEDE
Published: September 2019

The low yield of cabbage is often associated with the low content of soil organic matter. The inappropriate planting distance also contributes to the low yield of the cabbage plant. The provision of organic fertilizer with the right dosage and setting the right spacing is also expected to increase soil fertility and ultimately increase results of cabbage plants.   This research was conducted in the field from April to July 2018 in Subak Gede Bungan Kapal, Tunjuk Village, Tabanan District, Tabanan Regency. Treatment was arranged in a factorial manner in a Randomized Block Design (RBD). Two treatments studied in this study were plant spacing (30 cm x 40 cm; 30 cm x 40 cm; 30 x 50 cm) and organic fertilizer (Petroganic) (0 t ha-1; 1 t ha-1; 2 t ha -1; 3 t ha-1). The treatment was repeated three times.   The results showed that the interaction between the treatment of spacing and the use of doses of organic fertilizer had no significant effect on all observed parameters.   The treatment of spacing has a significant effect on the diameter of the commercial crop -1, the weight of fresh stover ha-1, and has a very significant effect on the weight of crop ha-1, and the weight of commercial crop ha-1. The highest weight of commercial k-ha ha-1 was obtained at a plant spacing of 30 x 50 cm (J3) of 525.86 kg, significantly increasing by 74.80% compared to a spacing of 30 x 30 cm (J1) which was 299.12 kg.  The treatment of organic fertilizer dosage has a very significant effect on the weight of crop ha-1, weight of commercial crop ha-1, commercial crop diameter-1 but has no significant effect on the fresh weight of stover ha-1. The highest weight of commercial ha-1krop obtained at the dose of organic fertilizer 2 t ha-1 of 444.23 kg (P2) increased by 58.30% compared to the dose of 0 t ha-1 (P0) of 280.62 kg