bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding cervical cancer awareness in hard-to-reach areas of Bangladesh: A cross-sectional study involving women and household decisionmakers
Authors: Nazrul Naheed , de Fouw Marlieke , Beltman Jogchum J. , de Zeeuw Janine , Schans Jurjen van der , Koot Jaap , Rasul Kazi Golam , Kulsum Mosamat Umma , Ahmed Md Shahnur , Rahman Anika , Rahman Aminur , Islam Kazi Maruful , Nessa Ashrafun , Campbell Christine , Stekelenburg Jelle
Published: August 2024

Introduction

In Bangladesh, the uptake of cervical cancer screening is low. Lack of knowledge and understanding of symptoms and risk factors contributes to low screening uptake. The purpose of this study was to explore the knowledge of cervical cancer risk factors and symptoms and to measure the association with socio-demographic characteristics among women and household decisionmakers living in hard-to-reach areas of Bangladesh.

Methods

A cross-sectional survey was conducted in five districts in Bangladesh among women aged between 30 and 60 years, their husbands, and their mothers-in-law from April to September 2022. Data were collected using a modified version of the validated AWACAN questionnaire tool. The significance level was considered at p-value <0.05 and odds ratios with 95% confidence.

Results

Nearly 50% of participating women in hard-to-reach areas of Bangladesh and their family decisionmakers had low levels of knowledge of the risk factors and symptoms of cervical cancer. Only 20% of respondents in our survey knew about HPV, the most important risk factor for developing cervical cancer. Most respondents were familiar with the terminology of cervical cancer as a disease; however, approximately 40% of respondents did not know that not adhering to cervical cancer screening could be seen as a risk factor. Women do not make decisions about participation in cervical cancer screening on their own. Knowledge of cervical cancer risk factors and symptoms among decisionmakers was significantly associated with higher education and higher household monthly expenditure.

Conclusion

Women, their husbands, and mothers-in-law in hard-to-reach areas of Bangladesh had limited knowledge about cervical cancer risk factors and symptoms. Engaging these key decision-makers in targeted health education is vital to improve screening uptake. Conduction of future research to identify and address screening barriers is also essential for effective prevention efforts.
Route to chaos and resonant triads interaction in a truncated rotating nonlinear shallow–water model
Authors: Carbone Francesco , Dutykh Denys
Published: August 2024

The route to chaos and the phase dynamics of the large scales in a rotating shallow-water model have been rigorously examined through the construction of an autonomous five-mode Galerkin truncated system employing complex variables, useful in investigating how large/meso-scales are destabilized and how their dynamics evolves and transits to chaos. This investigation revealed two distinct transitions into chaotic behaviour as the level of energy introduced into the system was incrementally increased. The initial transition manifests through a succession of bifurcations that adhere to the established Feigenbaum sequence. Conversely, the subsequent transition, which emerges at elevated levels of injected energy, is marked by a pronounced shift from quasi-periodic states to chaotic regimes. The genesis of the first chaotic state is predominantly attributed to the preeminence of inertial forces in governing nonlinear interactions. The second chaotic state, however, arises from the augmented significance of free surface elevation in the dynamical process. A novel reformulation of the system, employing phase and amplitude representations for each truncated variable, elucidated that the phase components present a temporal piece-wise locking behaviour by maintaining a constant value for a protracted interval, preceding an abrupt transition characterised by a simple rotation of ±π, even as the amplitudes display chaotic behaviour. It was observed that the duration of phase stability diminishes with an increase in injected energy, culminating in the onset of chaos within the phase components at high energy levels. This phenomenon is attributed to the nonlinear term of the equations, wherein the phase components are introduced through linear combinations of triads encompassing disparate modes. When the locking durations vary across modes, the resultant dynamics is a stochastic interplay of multiple π phase shifts, generating a stochastic dynamic within the coupled phase triads, observable even at minimal energy injections.
COVID-19 vaccination uptake in remote areas—Evidence from a panel survey in Bangladesh
Authors: Rudolph Lukas , Koubi Vally , Freihardt Jan
Published: August 2024

Background

Vaccination has proven to be an essential strategy in combating the COVID-19 pandemic. This study aims to discern the factors influencing both the intentions for and actual behavior regarding COVID-19 vaccination among remote, rural populations in Bangladesh.

Methods

The study utilized panel survey data comprising 1,698 randomly selected household heads. These are predominantly illiterate, of Muslim religion, middle-aged, and male, with agriculture or day labor as primary income source. They reside in 36 locations distributed along the whole 250 km length of the Jamuna River in Bangladesh. Data collection occurred through face-to-face and telephone interviews conducted between September 2021 and October 2022. Descriptive statistics and Ordinary Least Squares regression models were employed to assess influence factors for COVID-19 vaccination intentions and uptake. The analyses considered the constructs of the Health Belief Model alongside sociodemographic characteristics such as gender, age, religion, education, and income source.

Results

Survey respondents showed a notably high willingness to receive the COVID-19 vaccine promptly upon its availability. However, the effectiveness of the Health Belief Model in elucidating COVID-19 vaccination uptake was limited, except for its availability component. Older individuals, those with higher levels of education, and individuals employed in government or formal sector occupations were prompt in receiving the COVID-19 vaccine as it became available. Gender, religion, and the presence of dependents in the household did not exert a significant influence on vaccination uptake.

Conclusions

The results indicate that a strong willingness to receive the COVID-19 vaccine correlated with an increased likelihood of vaccine uptake once it was available. These findings suggest that a widespread distribution of COVID-19 vaccines to low-income and remote areas could have served as a vital strategy in mitigating the COVID-19 pandemic.
RNAseq analysis of oocyte maturation from the germinal vesicle stage to metaphase II in pig and human
Authors: Tang Feng , Hummitzsch Katja , Rodgers Raymond J.
Published: August 2024

During maturation oocytes at the germinal vesicle (GV) stage progress to metaphase II (MII). However, during in vitro maturation a proportion often fail to progress. To understand these processes, we employed RNA sequencing to examine the transcriptome profile of these three groups of oocytes from the pig. We compared our findings with similar public oocyte data from humans. The transcriptomes in oocytes that failed to progress was similar to those that did. We found in both species, the most upregulated genes in MII oocytes were associated with chromosome segregation and cell cycle processes, while the most down regulated genes were relevant to ribosomal and mitochondrial pathways. Moreover, those genes involved in chromosome segregation during GV to MII transition were conserved in pig and human. We also compared MII and GV oocyte transcriptomes at the isoform transcript level in both species. Several thousands of genes (including DTNBP1, MAPK1, RAB35, GOLGA7, ATP1A1 and ATP2B1) identified as not different in expression at a gene transcript level were found to have differences in isoform transcript levels. Many of these genes were involved in ATPase-dependent or GTPase-dependent intracellular transport in pig and human, respectively. In conclusion, our study suggests the failure to progress to MII in vitro may not be regulated at the level of the genome and that many genes are differentially regulated at the isoform level, particular those involved ATPase- or GTPase-dependent intracellular transport.
Evaluating variable selection methods for multivariable regression models: A simulation study protocol
Authors: Ullmann Theresa , Heinze Georg , Hafermann Lorena , Schilhart-Wallisch Christine , Dunkler Daniela ,   for TG2 of the STRATOS initiative
Published: August 2024

Researchers often perform data-driven variable selection when modeling the associations between an outcome and multiple independent variables in regression analysis. Variable selection may improve the interpretability, parsimony and/or predictive accuracy of a model. Yet variable selection can also have negative consequences, such as false exclusion of important variables or inclusion of noise variables, biased estimation of regression coefficients, underestimated standard errors and invalid confidence intervals, as well as model instability. While the potential advantages and disadvantages of variable selection have been discussed in the literature for decades, few large-scale simulation studies have neutrally compared data-driven variable selection methods with respect to their consequences for the resulting models. We present the protocol for a simulation study that will evaluate different variable selection methods: forward selection, stepwise forward selection, backward elimination, augmented backward elimination, univariable selection, univariable selection followed by backward elimination, and penalized likelihood approaches (Lasso, relaxed Lasso, adaptive Lasso). These methods will be compared with respect to false inclusion and/or exclusion of variables, consequences on bias and variance of the estimated regression coefficients, the validity of the confidence intervals for the coefficients, the accuracy of the estimated variable importance ranking, and the predictive performance of the selected models. We consider both linear and logistic regression in a low-dimensional setting (20 independent variables with 10 true predictors and 10 noise variables). The simulation will be based on real-world data from the National Health and Nutrition Examination Survey (NHANES). Publishing this study protocol ahead of performing the simulation increases transparency and allows integrating the perspective of other experts into the study design.
TAOK1-mediated regulation of the YAP/TEAD pathway as a potential therapeutic target in heart failure
Authors: Zhou Jiani , Wu Chaoqun , Zhao Miaohui
Published: August 2024

Background

This study aimed to determine the roles of interleukin (IL)-17, TAO kinase 1 (TAOK1), and NOD-like receptor protein 3 (NLRP3) in cardiomyocyte pyroptosis and proliferation.

Methods

The IL-17-treated H9C2 cells were used as in vitro heart failure (HF) models. These cells were subjected to TAOK1 overexpression or knockdown and treated with BMS-986299 (NLRP3 inflammasome agonist), MCC950 (NLRP3 inflammasome inhibitor), or verteporfin (Yes-associated protein [YAP] inhibitor). Thereafter, their pyroptosis, proliferative capacity, and gene and protein expression levels were detected. Doxorubicin-induced HF rats were used as in vivo models and subjected to TAOK1 overexpression. Thereafter, their myocardial pathology, NLRP3 inflammasome-mediated pyroptosis, and YAP/TEAD pathway function were evaluated.

Results

IL-17 treatment increased the pyroptosis and decreased the proliferative capacity of H9C2 cells. Additionally, IL-17 treatment inducedto the activation of the NLRP3 inflammasomes and inhibition of the YAP/TEAD pathway in the H9C2 cells. Moreover, the IL-17-mediated effects on the H9C2 cells were alleviated by TAOK1 overexpression and augmented by TAOK1 knockdown. Furthermore, treatment with BMS-986299 or verteporfin affected the pyroptosis, proliferative capacity, and NLRP3 inflammasome activation of the H9C2 cells independently of TAOK1 expression. In the doxorubicin-induced HF rat model, TAOK1 overexpression mitigated myocardial injury, suppressed NLRP3 inflammasome pathway activation, and restored the YAP/TEAD pathway activity.

Conclusion

TAOK1 played a crucial role in regulating IL-17-mediated increase in the pyroptosis and decrease in the proliferation of cardiomyocytes by regulating the activities of the NLRP3 inflammasomes and the YAP/TEAD pathway.
ActVI-ORFA directs metabolic flux towards actinorhodin by preventing intermediate degradation
Authors: Zhu Xuechen , Wang Rongbin , Siitonen Vilja , Vuksanovic Nemanja , Silvaggi Nicholas R. , Melançon III Charles E. , Metsä-Ketelä Mikko
Published: August 2024

The biosynthetic pathway of actinorhodin in Streptomyces coelicolor A3(2) has been studied for decades as a model system of type II polyketide biosynthesis. The actinorhodin biosynthetic gene cluster includes a gene, actVI-orfA, that encodes a protein that belongs to the nuclear transport factor-2-like (NTF-2-like) superfamily. The function of this ActVI-ORFA protein has been a long-standing question in this field. Several hypothetical functions, including pyran ring cyclase, enzyme complex stability enhancer, and gene transcription regulator, have been proposed for ActVI-ORFA in previous studies. However, although the recent structural analysis of ActVI-ORFA revealed a solvent-accessible cavity, the protein displayed structural differences to the well-characterized cyclase SnoaL and did not possess a DNA-binding domain. The obtained crystal structure facilitates an inspection of the previous hypotheses regarding the function of ActVI-ORFA. In the present study, we investigated the effects of a series of actVI-orfA test plasmids with different mutations in an established vector/host system. Time-course analysis of dynamic metabolism profiles demonstrated that ActVI-ORFA prevented formation of shunt metabolites and may have a metabolic flux directing function, which shepherds the flux of unstable intermediates towards actinorhodin. The expression studies resulted in the isolation and structure elucidation of two new shunt metabolites from the actinorhodin pathway. Next, we utilized computational modeling to probe the active site of ActVI-ORFA and confirmed the importance of residues R76 and H78 in the flux directing functionality by expression studies. This is the first time such a function has been observed for a member of NTF-2-like superfamily in Streptomyces secondary metabolism.
NeuroPi: A Portable Steady-State Visually Evoked Potential-based Brain-Computer Interface
Authors:   NTT Data, Barbosa Vitor M. , Carvalho Sarah N. ,   Technological Institute of Aeronautics (ITA), Leite Harlei M. A. ,   Technological Institute of Aeronautics (ITA)
Published: August 2024

The NeuroPi system is a Brain-Computer Interface (BCI) based on Steady-State Visually Evoked Potentials (SSVEP) that redefines human-machine interaction. This system employs brain activity responses induced by oscillatory visual stimuli to enable intuitive and efficient control of external devices. The system was designed to non-invasively gather brain signals using electroencephalography. A low-cost biosignal amplifier was employed to capture, filter, and digitize these signals. Python scripts were utilized for signal processing in the stages of preprocessing, feature extraction, and classification, ensuring seamless integration, customization, and straightforward adaptability. NeuroPi was designed with a focus on simplicity and user-friendliness, enabling the integration and control of electronic devices using brain signals. Additionally, the system is portable, cost-effective, and efficient, making it suitable for various real-world applications. Performance tests validate NeuroPi's effectiveness, highlighting its potential to contribute to the popularization of SSVEP-based BCI systems. The NeuroPi system is available to be freely used for research and development purposes.
Cidade, escola e letramentos de reexistência
Authors: Lima Ana Luiza Silva , Carmim Lavareda Welton Diego
Published: August 2024

Trata-se de uma proposta que analisa a potencialidade didática de letramentos de reexistência, na cena periférica da cidade de Belém do Pará, a partir de enunciados transversais. O processo de teorização dos conceitos ocorrerá por meio de um diálogo entre Souza (2011), Canevacci (2004) e Fiorin (2016) na busca de desmembrar gestos interpretativos que tornem mais amplo o olhar sobre os espaços habitados periféricos de uma dada espacialização urbana em consonância com a sala de aula do 1° ano do Ensino Médio. Para sistematizar a pesquisa, os procedimentos técnicos do estudo têm abordagem qualitativa, tendo como instrumento para a produção dos dados as brechas discursivas deixadas na espacialização “marginal” da cidade. Almeja-se, com este artigo, também repensar estratégias didáticas que aproximem mais o estudante do ambiente escolar e, ao mesmo tempo, fortalecer uma prática de pesquisa ativa à formação da nossa própria subjetividade enquanto pesquisadores mergulhados em espaços notadamente mais plurais.
A extensão com estudantes de licenciatura: um estudo de caso a partir da Teoria da Subjetividade
Authors: Gonzalez Rafael , Goulart Daniel Magalhães
Published: August 2024

La extensión ofrece un espacio fecundo para el proceso de formación docente. Esta investigación aborda la dimensión subjetiva de la participación en acciones de extensión por estudiantes de profesorado. El estudio se realizó desde la perspectiva de la Teoría de la Subjetividad en una perspectiva histórico-cultural de González Rey, y de su Metodología Constructivo - Interpretativa. A partir de un estudio de caso, se buscó comprender cómo se configura subjetivamente la participación de estudiantes de profesorado en acciones de extensión, así como posibles recursos subjetivos que son generados en la participación en estas experiencias. Se concluye que la implicación de estudiantes de profesorado en prácticas extensionistas que se desarrollan en contextos comunitarios, llevaron a cambios subjetivos, que favorecieron el aprendizaje de la práctica docente y la producción de recursos subjetivos.
Formação de habilidades em Modelagem Matemática fundamentada no sistema didático Galperin, Talízina, Majmutov para licenciandos em Matemática.
Authors: Wakiyama Yachiko Nascimento
Published: August 2024

O presente texto apresenta, nos limites do resumo, os resultados do trabalho de doutoramento que trata da formação das habilidades requeridas no processo de modelagem matemática para licenciandos de Matemática da Universidade Federal do Amazonas (UFAM).
Prize Effect: an analysis of the Nota Fortaleza program
Authors: Da Cunha Júnior José Maria , Brito Soares Ricardo , Cristina Silva de Azevedo Débora
Published: August 2024

The purpose of this article is to analyze whether the Nota Fortaleza program fulfills its role in terms of the continuous engagement of consumers in the inspection process, since they are awarded. For this, we use a panel data model, considering different intercepts in order to control effects not observed in cross-section units. The estimated coefficients for the main indicators were statistically higher than those observed for the lagging indicators for all levels of awards, associated with a hypothesis that the contemplation in the draw affects their behavior in a future analysis window.
Economic Impacts of Natural Disasters in Brazilian Municipalities and the Focus of the BNDES PER Program
Authors: Agostini Martini Ricardo , Campello Gallo Maria Eduarda
Published: August 2024

The aim of this study is to evaluate the targeting of the BNDES Emergency Program for the Reconstruction of Municipalities Affected by Natural Disasters (BNDES PER) in Brazilian municipalities. The impact of natural disasters on economic activity in the municipalities was estimated using the synthetic control methodology. The program's targeting was estimated using logistic regression and fixed effects methods, taking the estimated impact as the regressor. Looking at data from 2008 to 2017, it was found that natural disasters had a negative impact on the GDP per capita of most of the affected municipalities, with effects lasting up to three years after the events. On the other hand, the BNDES PER was able to reach the most intensely impacted municipalities in terms of GDP per capita and value added by services per capita.
A história dos Kaingang da Terra Indígena Linha Glória, Estrela, Rio Grande do Sul/Brasil: Sentidos de sua (re)territorialidade
Authors: Sehn da Silva Juciane Beatriz , Da Silva Laroque Luís Fernando
Published: August 2024

O presente trabalho versa sobre a história do grupo indígena Kaingang da Terra Indígena Linha Glória, localizada no município de Estrela, Vale do Taquari, Rio Grande do Sul, no que se refere as suas movimentações pelo tradicional território Kaingang. Por meio deste estudo, temos a intenção de mostrar que este grupo, apesar de ter permanecido fora das terras indígenas tomadas como oficiais pelo Estado Nacional, onde residem muitos de seus parentes, continuou a vivenciar práticas culturais da tradição Kaingang. O objetivo do estudo é também demonstrar que estes indígenas têm sido agentes de sua própria historicidade e os sentidos pelos quais envolvem a sua territorialidade têm a ver com a cosmologia tradicional Kaingang, que se constitui numa verdadeira riqueza cultural e patrimonial para o Vale do Taquari.
Seneca’s Phaedra 406–430 and the E-branch of the MS Tradition
Authors: Słomak Iwona
Published: August 2024

This paper aims to revise previous findings concerning lines 406–430 of Seneca’s Phaedra. Referring to the manuscript tradition, it demonstrates that the heading before 406 and the marginal notation used to identify speaking characters may have been misinterpreted as a result of this notation having been erased from codex Etruscus, the main representative of one of the two branches of the manuscript tradition of Seneca’s plays. Next, it argues that because of the formal consistency of this codex reading (E) – as contrasted with the inconsistencies in A – and the fact that E makes satisfactory sense here, the reconstructed reading of Etruscus should be taken into consideration in future editions of Seneca’s drama.
Epitafium nagrobne biskupa Andrzeja Szołdrskiego z katedry poznańskiej w świetle jego testamentu (Bishop Andrzej Szołdrski’s Tombstone Epitaph from the Poznań Cathedral in the Light of His Will)
Authors: Barbarzak Dawid
Published: August 2024

The Latin tombstone inscription dedicated to Andrzej Szołdrski, the bishop of Poznań (c. 1583–1650), mainly commemorates the work of rebuilding the cathedral destroyed in a fire in 1622, as well as his generous donations for church and charity purposes. A comparison of the inscription with the bishop’s will reveals many similarities between both texts. The fact that the authors of the inscription were also the executors of the will greatly influenced the content of the epitaph, as well as the entire appearance of the chapel. Both texts contain typical Baroque concepts related to the passing of time (vanity, theatrum mundi). However, while the bishop downplays the role of his earthly achievements in the will, the epitaph, according to the tradition of this epigraphic genre, belongs to enkomion (laudatio).
Penitencia cuaresmal como medicina del alma. Nueva edición de un sermón de Cuaresma de San Vicente Ferrer
Authors: Rubio Josep E.
Published: August 2024

The sermons preached in Valencia by Saint Vincent Ferrer during Lent in the year 1413 are an excellent model of the type of preaching carried out by the Valencian Dominican. This article offers a critical edition of one of these sermons (number XXVI, with the theme “Erat praedicans in synagogis Galilaeae”) accompanied by explanatory notes on its structure and content, as well as the identification of the theological sources used by the preacher. Thus, a model is offered for a complete reissue of the sermonary, the need for which is evident in the modifications introduced with respect to previous editions.
Amor y diálogo en la trama trovadoresca
Authors: González Déborah
Published: August 2024

In the cantigas de amor – the main genre adopted by Galician-Portuguese troubadours – the speech uttered by the lyrical voice of the male lover in confessing his love or love-sorrow occasionally appears in alternation with the voice of the lady, who intervenes to offer her response. Our attention here will focus, in the first place, on the compositions in which a direct alternation of these voices can be found within the stanzas, as well as on Airas Moniz’s cantiga spoken in cobras. Due to the particularities of the latter at a structural and formal level, this alternation can be seen in relation to the definition of the tenção of the Galician-Portuguese poetic treatise Arte de Trovar, which indicates that cantigas of this type «se podem fazer d'amor ou d'amigo ou d'escarnho ou de maldizer» (can be used for love or friendship or to mock or curse). Our interest in the dialogic model extends to the non-fictional tenções between troubadours, since in several of these the adoption and/or recreation of motifs and topics equally associated with courtly love can be seen.
Origine dell’it. anche e dei suoi correlati romanzi
Authors: Nocentini Alberto
Published: August 2024

The origin of it. anche ‘also, too’ and of its Romance cognates is commonly traced back to Protoromance *anque, a reconstructed form, which is interpreted as a derivative of the dubitative Latin particle an. This explanation turns out to be untenable, because it does not account for the primitive meanings of anche and its Romance cognates, that is ‘still’ and ‘never’, as is evident from the oldest records of the Romance languages. Therefore, Nicholson’s hypothesis of a derivation from Latin antequam is reconsidered through the meanings of ‘before this time’, ‘up to this time’, which later evolved to ‘still, yet’ and ‘never’ in a negative context. The mechanism whereby a conjunction may become an adverb through the ellipsis of the verb is shown by quoting parallel examples in Italian.
Generalized encoding of the relative subjective value of cognitive effort in dorsal ACC
Authors: Crawford Jennifer L. , Brough Rachel E. , Eisenstein Sarah A. , Peelle Jonathan E. , Braver Todd S.
Published: August 2024

Making choices about whether and when to engage cognitive effort are a common feature of everyday experience, with important consequences for academic, career, and health outcomes. Yet, despite their hypothesized importance, very little is understood about the underlying mechanisms that support this form of human cost-benefit decision-making. To investigate these mechanisms, we used the Cognitive Effort Discounting Paradigm (Cog-ED) during fMRI scanning to precisely quantify the neural encoding of varying cognitive effort demands relative to reward outcomes, within two distinct cognitive domains (working memory, speech comprehension). The findings provide strong evidence that the dorsal anterior cingulate cortex (dACC) plays a central and selective role in this decision-making process. Trial-by-trial modulations in dACC activation tracked the relative subjective value of the low-effort, low-reward option, with the strongest activity occurring when this was of greater value than the high-effort, high-reward option. In contrast, dACC activity was not modulated by decision difficulty, though such effects were found in other frontoparietal regions. Critically, dACC activity was also strongly correlated across the two decision-making task domains, and further predicted subsequent choice behavior in both. Together, the results suggest that dACC activity modulation reflects a domain-general valuation comparison mechanism, which acts to bias participants away from decisions to engage in cognitive effort, when the perceived subjective costs of such engagement outweigh the reward-related benefits. These findings complement work in other cost domains and species by pointing to a clear role of dACC in representing subjective value differences between choice options during cost-benefit decision-making.

Significance StatementDespite the growing popularity of decision-making paradigms to study the neural mechanisms underlying choices to engage in cognitive effort, there is still little consensus about how such decisions are implemented in the human brain. This study used two distinct versions of a cognitive effort decision-making paradigm during fMRI scanning to identify generalized brain mechanisms engaged while participants decided whether to engage in cognitive effort. Importantly, the results provide additional evidence that the dorsal anterior cingulate cortex is a key locus of cognitive effort-based decision-making. Specifically, this brain region signals the difference in relative value across choice options, making it an attractive target for future studies aimed at investigating the altered decision-making observed in populations for which apathy is commonly observed.
Cross-Border Trade Disputes: A Comparative Analysis of Indonesia and Australia
Authors: Riyanto R Benny , Laryea Emmanuel , Fibrianti Nurul , Latifiani Dian
Published: August 2024

This comparative analysis delves into the intricate landscape of cross-border trade disputes, focusing on Indonesia and Australia. As two significant players in the global trade arena, understanding their dispute resolution mechanisms is crucial for fostering bilateral and multilateral trade relationships. The study examines the legal frameworks, institutional mechanisms, and cultural factors influencing trade dispute resolution in both countries. Indonesia's approach may reflect its unique socio-political context, while Australia's system could showcase its commitment to transparency and adherence to international trade norms. Key aspects explored include the role of governmental bodies, such as trade ministries and dispute settlement panels, as well as the utilization of alternative dispute resolution methods like arbitration and mediation. Moreover, the analysis delves into the effectiveness and efficiency of these mechanisms in resolving disputes promptly while maintaining fairness and equity for all parties involved. Comparisons are drawn between Indonesia and Australia in terms of legal precedents, enforcement mechanisms, and the level of stakeholder engagement in the dispute resolution process. Additionally, cultural nuances and historical contexts are considered to understand how these factors shape the negotiation and resolution of trade conflicts. By synthesizing these insights, the study aims to provide policymakers, trade practitioners, and academics with a comprehensive understanding of the strengths and weaknesses of each country's approach to cross-border trade dispute resolution. Ultimately, it seeks to contribute to the development of best practices and strategies for enhancing trade relations and promoting economic cooperation between Indonesia and Australia, as well as other nations globally.
Decreased sirtuin 4 levels promote cellular proliferation and invasion in papillary thyroid carcinoma
Authors: Lee Hyun-Jin , Hah Young-Sool , Cheon So Young , Won Seong Jun , Yim Chae Dong , Ryu Somi , Lee Seung-Jun , Seo Ji Hyun , Park Jung Je
Published: August 2024

Objective

This study examined the effect of sirtuin 4 (SIRT4), a NAD+-dependent deacetylase, on the proliferation and progression of papillary thyroid carcinoma (PTC).

Methods

Data from The Cancer Genome Atlas (TCGA) were analyzed to identify SIRT4 expression in thyroid cancer. Subsequently, the correlation between SIRT4 expression and clinical characteristics was examined in 205 PTC tissue samples. In vitro assays using three human thyroid cancer cell lines (B-CPAP, TPC-1, and SNU-790) were conducted to assess the effects of regulated SIRT4 expression on cell growth, apoptosis, invasion, and migration. Furthermore, in vivo experiments were performed in a xenograft mouse model.

Results

Gene Expression Omnibus (GEO) and TCGA data indicated that SIRT4 expression is lower in thyroid cancer and SIRT4 downregulation is associated with poor overall survival. In PTC tissues, positive SIRT4 expression was associated with decreased extracapsular extension. In in vitro experiments using three human thyroid cancer cell lines, overexpression of SIRT4 decreased cell survival, clonogenic potential, and invasion and migratory capabilities, as well as inducing apoptosis and increasing reactive oxygen species levels. SIRT4 overexpression upregulated E-cadherin and downregulated N-cadherin, suggesting its potential involvement in the regulation of epithelial–mesenchymal transition. These findings were confirmed in vivo using a xenograft mouse model.

Conclusion

This study provides novel insight into the potential contribution of SIRT4 to the regulation of the pathological progression of PTC. The data suggest that SIRT4 plays a tumor-suppressive role in PTC by inhibiting growth, survival, and invasive potential. Future research should investigate the molecular mechanisms underlying these effects of SIRT4.
Investigate the Push-out Bond Strength of Root Canal Fillings After Calcium Hydroxide Medicament Removal with Ethanol
Authors: Fundaoğlu Küçükekenci Funda
Published: August 2024

Aim: The present study aims to evaluate the effect of various irrigation solutions used to remove calcium hydroxide [Ca(OH)2], especially ethanol, on the push-out bond strength (PBS) of root canal filling.
Materials And Methods: 50 human incisor protaper next X3 files were prepared. Root canals were filled with Ca(OH)2. The teeth were kept at 37 °C and 100 % humidity for 1 week. The samples were divided into 5 experimental groups according to the irrigation solution used to remove the Ca(OH)2 (n=10); Group 1: 2.5 % NaOCl, Group 2: 6 mL 17 % EDTA, Group 3: 6 ml 37 % phosphoric acid, Group 4: 6 ml 70 % ethanol, Group 5: 6 ml distilled water. After removing the Ca(OH)2, the root canals were filled with AH plus root canal sealer and gutta-percha. PBS test was applied by taking one sample from each tooth from the coronal, middle, and apical thirds. The data were analyzed with a one-way analysis of variance and Tukey's multiple comparison test (α=0.05).
Results: Statistically, the highest bond strength was found in the ethanol group (6.46±0.14 MPa), and there was a statistical difference between all groups (p
The Relationship Between the Amount of Peri-implant Keratinized Mucosa and Patient Satisfaction
Authors: Jafarov Jeyhun , Aykaç Yaşar , Yeğinoğlu Sevilay , Tezel Özge , Akkaya Mehmet Murat
Published: August 2024

Background: The aim of this study was to evaluate the relationship between periimplant soft tissue health, the amount of periimplant keratinized mucosa, and the satisfaction of patients who applied to the Ankara University Faculty of Dentistry and had a fixed implant-assisted restoration in the premolar and/or molar region at least 1 year after the functional loading of implant treatment.
Methods: 40 implants from 40 patients, of which 24 were female and 16 were male, were included in our study. Periimplant soft tissue health was objectively evaluated with clinical measurements and a radiographic examination. The amount of keratinized mucosa around the implants was measured and recorded. Afterwards, the patients were asked to fill out a (subjective) questionnaire that included socio-demographic data and evaluated their satisfaction with their implants by VAS.
Results: All patients in the healthy group had keratinized mucosa. In this study, a mean total VAS score of 8.40 out of 10 indicates that patients are satisfied with implant treatments. However, the relationship between chewing, speech, aesthetics, pain and discomfort, the presence of bleeding scores, and the total VAS score was statistically significant.
There was a statistically significant difference between those without keratinized mucosa, those with inadequate keratinized mucosa, and those with present/adequate keratinized mucosa in terms of speech VAS score (p=0.050).
Conclusions: In our study, the presence of keratinized mucosa significantly affected VAS scores at the speech level.
KEYWORDS
Dental implants, oral mucosa, patient satisfaction, periimplant
Diş Hekimliği Öğrencilerinin İlaca Bağlı Olarak Çene Kemiklerinde Görülen Osteonekroz (MRONJ) ile İlgili Bilgi Düzeylerinin Değerlendirilmesi
Authors: Şahin Meryem , Doğan Fatma Büşra , Demir Azize
Published: August 2024

Amaç: Başta kemik metastazı olan kanser hastalarının tedavisi olmak üzere osteoporoz, Paget hastalığı, multiple myelom gibi patolojilerin tedavisinde kullanılan bifosfanatların ve benzeri özellikteki antianjiojenik ve antirezorptif ilaçların istenmeyen bir yan etkisi çenelerde görülen osteonekrozdur. Bu çalışmada amaç diş hekimliği öğrencilerinin ilaçla ilişkili çene osteonekrozu (MRONJ) ile ilgili bilgi düzeylerini değerlendirmek, farkındalık oluşturmak aynı zamanda diş hekimliğinde MRONJ’un önemini vurgulamaktır.
Gereç ve Yöntemler: Çalışma için gerekli etik onay alındıktan sonra öğrencilere anket çalışması hakkında bilgi verilmiştir. MRONJ tanımı, bifosfanat ve benzeri ilaçların kullanım endikasyonları, ilaçların kullanım süresi, dozajı ve kullanım şeklinin önemini sorgulayan, 10 sorudan oluşan anket soruları çalışmaya katılmayı kabul eden öğrencilere dağıtılmış, cevaplama sırasında herhangi bir veri kaynağına izin verilmemiştir. Verilerin analizi IBM SPSS 25 programı kullanılarak yapılmıştır.
Bulgular: Çalışmaya 102 4.sınıf öğrencisi, 100 5.sınıf öğrencisi olmak üzere toplam 202 öğrenci katılmıştır. Terminolojinin sorgulandığı ilk soruda en fazla bilinen terimin % 98’lik oranla BRONJ olduğu görülmektedir. MRONJ tanısı için gerekli üç kriteri de doğru cevaplayan 42 öğrenci, hiçbir kriteri cevaplamayan 36 öğrenci mevcuttur. Bu kriterlerin tamamını bilen öğrencilerin diğer sorulara daha doğru cevaplar verdiği görülmüştür.
Sonuçlar: Bu çalışma öğrencilerin tamamına yakınının anamnezde çene osteonekrozuna neden olan ilaç kullanımını sorgulamayı ve hastaların bu ilaçlarla tedaviye başlamadan önce diş hekimine muayene olmalarını önemli gördüğünü göstermektedir. Bununla birlikte MRONJ tanısı, risk faktörleri, neden olan ilaçlar ve kullanıldığı hastalıklar yönünden yeterli bilgi düzeyine sahip olmadıkları da görülmektedir.
Anahtar Kelimeler: Osteonekroz, bifosfonat, denosumab
Çocuk Hastaların Diş Hekimliği Kliniğine Başvuru Zamanlarının ve Sebeplerinin Değerlendirilmesi
Authors: Açıkgöz Gözde
Published: August 2024

Amaç: Pamukkale Üniversitesi Diş Hekimliği Fakültesi Ağız, Diş ve Çene Radyolojisi kliniğine başvuran çocuk hastaların başvuru sebeplerinin ve ilk kez diş hekimine başvuru yaşının araştırılmasıdır.
Gereç ve Yöntemler: Çalışmaya Ekim 2022-Mart 2023 tarihleri arasında kliniğimize başvuran 0-15 yaş aralığındaki 968 çocuk hasta dahil edildi. Demografik bilgiler, diş hekimine başvuru sebepleri ve ilk diş hekimi ziyareti ile ilgili veriler ebeveynlerden öğrenildi. Başvuru sebepleri dokuz ana gruba (dental ağrı, diş çürüğü, dental travma, diş çekimi, renk değişiklikleri/lekeler, ortodontik tedaviler, patolojiler, koruyucu tedaviler, rutin kontrol muayenesi) ayrıldı.
Bulgular: 968 çocuk hastadan 88’i (% 9.1) ilk kez diş hekimi ziyaretine gelmişti. İlk diş hekimi ziyaretine gelen çocuk hastaların çoğu 3-5 yaş aralığında olup, ortalama yaş 6.01 ± 3.27 idi. Kliniğimize en sık başvuru sebebi 485 kişi (% 50.1) ile diş çürüğü iken, bunu 226 kişi (% 23.3) ile dental ağrı takip etti. Koruyucu tedaviler için başvuran hastaya rastlanmadı. İlk diş hekimi ziyaretinin en sık sebebi ise 49 kişi (% 55.7) ile diş çürüğüydü.
Sonuç: Çocuk hastalar diş hekimine pek çok nedenden dolayı başvursa da en sık başvuru nedeninin diş çürükleri olduğu görülmüştür. Ayrıca çocuk hastaların çoğu ilk diş hekimi ziyaretini olması gerekenden geç gerçekleştirmektedir. Çocuklarda bu yaşın daha erkene çekilmesi ve ebeveyn farkındalığının arttırılması gereklidir.
Anahtar Kelimeler: Başvuru; çocuk hasta; ilk diş hekimi ziyareti
Chlorophyll, Carotene, and Anthocyanin Effect on Color Change of Resin-Based Dental Materials: An in-Vitro Study
Authors: Elter Bahar , Diker Burcu
Published: August 2024

Background: The purpose of the present study was to evaluate the effect of chlorophyll, carotene, and anthocyanin-containing juices on color stability of resin-based dental materials.
Methods: 192 disc-shaped specimens were prepared from six resin-based materials (universal, anterior, and flowable resin composites; self-curing polymethyl methacrylate (pmma), bis-acrylic composite, and computer-aided design/computer-aided manufacturing(CAD/CAM)- fabricated pmma). Divided groups were immersed in distilled water, spinach, carrot, and black mulberry juices (n=8). Color measurements were taken with a spectrophotometer before the immersions(T0), after one week(T1), and after four weeks(T2). The color differences (ΔE00) were used for statistical analysis. ANOVA was used to determine the effects of material, solution, and time on color change.
Results: The highest color change was recorded for the CAD/CAM-fabricated pmma (ΔE=2.67) (p
Farklı Modlarda Kullanılan LED Işık Cihazı ile Polimerize Edilen Güncel Rezin Kompozitlerin Renk Stabilitesinin Değerlendirilmesi
Authors: Yenidünya Özge Gizem , Yazkan Başak , Akgül Nilgün , Şahin Mehmet Alperen
Published: August 2024

Amaç: Bu çalışmada, farklı modlar ile polimerize edilen güncel bulk-ﬁll kompozitlerin, kahve solüsyonunda bekletilmesi ile meydana gelen renk değişimlerinin geleneksel bir rezin kompozit ile CIEDE2000 formülasyonu kullanılarak karşılaştırmalı olarak incelenmesi amaçlanmıştır.
Gereç ve Yöntem: Üç farklı restoratif materyalin [GrandioSO (GSO), GrandioSO x-tra (GSX), VisCalor bulk (VCB)] her birinden 40 örnek olmak üzere, 8×4 mm kalıplar kullanılarak 120 adet disk şeklinde örnek hazırlandı. Her bir kompozit grubu LED ışık kaynağının yüksek güç (1200 mW/cm2) ve turbo (2100 mW/cm2) modları talimatlarda belirtilen sürelerce uygulanarak polimerize edildi (n=20). Ardından her bir grup, iki farklı içecekte (kahve ve distile su) bekletilmek üzere alt gruplara ayrıldı (n=10). Renk değişim değerleri [CIEDE2000 (ΔE00)] 1., 7. ve 30. günlerin sonunda hesaplandı. ΔE00 değerlerinin karşılaştırılmasında p
Dişli ve Posterior Kısmi Dişsiz Hastalar Arasında Masseter Kas Kalınlığının ve Mandibulanın Morfolojik Özelliklerinin Karşılaştırılması
Authors: Demir Hilal , Yaşar Füsun
Published: August 2024

Amaç: Dişli ve posterior kısmi dişsiz hastaların masseter kas kalınlıkları ve mandibular morfolojilerinin karşılaştırılmasıydı.
Gereç ve Yöntemler: 40-60 yaş arası 90 kadın hasta, dişli ve posterior dişsiz olmak üzere ikiye ayrıldı. Çalışmaya katılan bireylerin herhangi bir iskelet kas hastalığı, bruksizm alışkanlığı yoktu. Kontrol grubu ve bilateral posterior dişsiz hastalarda ölçümler çift taraflı yapıldı ve sağ sol taraf ölçümlerinin ortalamaları alındı. Tek taraflı posterior dişsiz hastaların sadece dişsiz olan tarafları ölçüldü. Masseter kası kalınlığını değerlendirmek için, ultrasonografi ile, istirahat ve ısırma pozisyonlarında, aynı gözlemci tarafından beş dakika aralıklarla iki kez ölçüm yapıldı. Mandibular morfolojinin yorumlanması için, panoramik radyograf üzerinde gonial ve antegonial açılar ölçüldü. Aynı gözlemci bir hafta sonra ikinci kez ölçümlerini tekrarladı.
Bulgular: Masseter kası kalınlığı ile posterior kısmi dişsizlik arasında herhangi bir anlamlı ilişki bulunamadı (ısırma durumunda p=0,793, serbest durumda p=0,962). Pearson korelasyon testi sonuçlarına göre hem kontrol hem çalışma grubunda gonial açı ile masseter kas kalınlığı arasında anlamlı bir negatif korelasyon vardı (-0,402≤r≤-0,375).
Sonuç: Posterior kısmi dişsizlik, masseter kas kalınlığında anlamlı bir azalmaya neden olmamıştır. Masseter kasının fonksiyonel aktivitesi, gonial bölgenin şekillenmesinde etkilidir. Ultrasonografi, masseter kası kalınlığının değerlendirilmesinde güvenilir, doğru ve tekrarlanabilir bir yöntem olarak bulunmuştur.
Anahtar kelimeler: Antegonial açı, Gonial açı, Masseter kası, Panoramik radyograf, Ultrason.
Ebeveyn Farkındalığının Genel Anestezi Altında Günübirlik Pediyatrik Diş Tedavisi Öncesi Açlık Uyumuna Etkisi: Prospektif Gözlemsel Çalışma
Authors: Arun Funda
Published: August 2024

ÖZ
Amaç
Çocuk hastalarda pulmoner aspirasyon riskini en aza indirmek için ameliyat öncesi açlıkla ilgili kılavuzların önerileri uygulanır. Bu çalışma genel anestezi (GA) altında günübirlik diş tedavisi yapılan çocuklarda ebeveyn farkındalığının ameliyat öncesi açlık uyumuna etkisini değerlendirmek ve bunu etkileyen faktörlerin belirlenmek amacıyla planlandı.
Gereç ve Yöntemler
Bu çalışmaya ASAI-II, 88 çocuk hastanın ebeveynleri dahil edildi. Çalışmanın birinci kısmında, ebeveynler çocuklarının ameliyat öncesi açlığı ile ilgili talimatları anestezi öncesi konsültasyon sırasında hem sözlü hem de yazılı talimatlar yoluyla aldılar. İkinci kısmında hastalar GAi ameliyathanesine geldiğinde, cerrahi prosedürden sorumlu anestezi uzmanı tarafından ebeveynlere doldurmaları için anket formu verildi.
Bulgular
Hastalarda GA öncesi açlık süreleri katı gıdalar için ortalama 10 saat, berrak sıvılar için 8 saat bulundu. Ebeveyn eğitim durumu lise olan hastaların katı gıda açlık süreleri ortalama 11 saat ile diğer gruplardan daha uzun bulundu (p=0,017). ‘Hastanızın aç bırakılma sebebi ne olabilir?’ sorusuna “Kusma riski” ve “Gıdaların solunum yoluna kaçma riski” cevabı veren ebeveynlerin hastalarında sıvı açlık süreleri diğer cevapları verenlere göre daha kısa bulundu (p=0,002). ‘Hastanız Neden Önerilenden Uzun Süre Aç Kaldı?’ sorusuna 64 (%72,7) ebeveyn ‘Uykusunu Bölmek İstemedim’, 24 (%27,3) ebeveyn ‘Sebebi Yok’ yanıtını verdi.
Sonuç
Ameliyat öncesi dönemde çocukların açlık sürelerine ilişkin ebeveynlerin daha iyi uyum sağlaması ve endişelerinin giderilmesi için anlaşılır talimatlar, hatırlatma aramaları, eğitici videolar ve iletişim kaynaklarının sağlanması gereklidir.
Anahtar Kelimeler
Açlık; ebeveyn farkındalığı; çocuk hasta; ameliyat öncesi dönem
Dental İmplant Uzunluğunun Belirlenmesinde Panoramik Radyografi Etkinliğinin Değerlendirilmesi
Authors: Aktı Ahmet , Dolunay Uğur , Gürses Gökhan , Küçükkolbaşı Hasan , Alıç Merve , Körez Muslu Kazım
Published: August 2024

Giriş: Panoramik radyografiler, dental implant tedavisi öncesi değerlendirme ve tedavi protokollerinin hazırlanmasında radyografik bir yöntem olarak sıklıkla kullanılmaktadır. Bu retrospektif çalışmanın amacı, maksiller ve mandibular posterior bölgeye dental implant yapılmış hastalarda, preoperatif panoramik radyografik görüntüler üzerinde yapılan planlamaya göre belirlenen implant boyları ile yerleştirilmiş implant boyları arasındaki korelasyonu incelemek ve panoramik radyografinin bu kapsamda güvenilirliğini ve yeterliliğini değerlendirmektir.
Materyal ve Metod: Çalışmada, Mart 2023 ile Şubat 2024 tarihleri arasında dental implant tedavisi için kliniğimize başvuran 568 hastanın dosyası incelenmiştir. Bu bağlamda, maksiller ve mandibular posterior bölgeye implant yapılmış ve preoperatif panoramik radyografisi olan hastalar çalışmaya dahil edilmiştir. Radyografik görüntülerde uzunluk ölçümünü engelleyen artefaktlar bulunan, açılı implant yapılmış, eksternal ve internal sinüs lifting yapılmış hastalar ve vertikal augmentasyon yapılmış hastalar çalışma dışı bırakılmıştır. İstatiksel analizler için SPSS 23 (SPSS Inc., Chicago, IL, USA) yazılımı kullanılmıştır.
Bulgular: Çalışmaya, toplamda 234 hastada 443 dental implant dahil edilmiştir. Örneklemdeki hastaların cinsiyet dağılımı, %54'si (n=126) kadın ve %46’si (n=108) erkek hastadan oluşmaktadır. İmplantların %46,7’si (n=207) maksiller posterior %53,3’si (n=236) mandibular posterior bölgeye uygulanmıştır. Planlanan implant boyları ile yapılmış implantlar arasındaki intraclass correlation coefficient uyum katsayısı 0,9014 olarak bulunmuştur. Erkekler arasında bu oran 0,9036; kadınlar arasında 0,9 olarak ölçülmüştür. Bu sonuçlar, planlanan ve uygulanan implant boyları arasında yüksek düzeyde bir uyum olduğunu göstermektedir.
Sonuç: Panoramik radyografi, dental implant tedavisi öncesinde implant uzunluğunun belirlenmesinde basit, kolay ulaşılabilir ve yararlı bir araç olarak kabul edilebilir. Ancak planlamayla daha uyumlu implant tedavisi için konik ışınlı bilgisayarlı tomografi gibi ileri radyografik tetkiklere de ihtiyaç bulunmaktadır.