bluuebunny/crossref_metadata_2025 huggingface dataset:
Glaucomatous Maculopathy: Thickness Differences on Inner and Outer Macular Layers between Ocular Hypertension and Early Primary Open-Angle Glaucoma Using 8 × 8 Posterior Pole Algorithm of SD-OCT
Authors: Garcia-Medina Jose , del-Rio-Vellosillo Monica , Palazon-Cabanes Ana , Pinazo-Duran Maria , Zanon-Moreno Vicente , Villegas-Perez Maria
Published: May 2020

The purpose of this study was to compare the thickness of all inner and outer macular layers between ocular hypertension (OHT) and early primary open-angle glaucoma (POAG) using spectral domain optical coherence tomography (SD-OCT) 8 × 8 posterior pole algorithm (8 × 8 PPA). Fifty-seven eyes of 57 OHT individuals and fifty-seven eyes of 57 early POAG patients were included. The thickness of macular retinal nerve fiber layer (mRNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform and nuclear layer, photoreceptor layer (PRL) and retinal pigment epithelium were obtained in 64 cells for each macular layer and mean thickness of superior and inferior hemispheres was also calculated. Thinning of superior and inferior hemisphere mean thickness in mRNFL, GCL and IPL and thickening of superior and inferior hemisphere mean thickness in PRL and inferior hemisphere in INL were found in early GPAA group. Otherwise, heatmaps representing cell-to-cell comparisons showed thinning patterns in inner retinal layers (except for INL) and thickening patterns in outer retinal layers in GPAA group. We found that 8 × 8 PPA not only allows the detection of significant thinning patterns in inner retinal layers, but also thickening patterns in outer retinal layers when comparing early POAG eyes to OHT eyes.
Reproducibility of Ablated Volume Measurement Is Higher with Contrast-Enhanced Ultrasound than with B-Mode Ultrasound after Benign Thyroid Nodule Radiofrequency Ablation—A Preliminary Study
Authors: Schiaffino Simone , Serpi Francesca , Rossi Duccio , Ferrara Valerio , Buonomenna Ciriaco , Alì Marco , Monfardini Lorenzo , Sconfienza Luca Maria , Mauri Giovanni
Published: May 2020

The reproducibility of contrast-enhanced ultrasound (CEUS) and standard B-mode ultrasound in the assessment of radiofrequency-ablated volume of benign thyroid nodules was compared. A preliminary study was conducted on consecutive patients who underwent radiofrequency ablation (RFA) of benign thyroid nodules between 2014 and 2016, with available CEUS and B-mode post-ablation checks. CEUS and B-mode images were retrospectively evaluated by two radiologists to assess inter- and intra-observer agreement in the assessment of ablated volume (Bland–Altman test). For CEUS, the mean inter-observer difference (95% limits of agreement) was 0.219 mL (-0.372–0.809 mL); for B-mode, the mean difference was 0.880 mL (-1.655–3.414 mL). Reproducibility was significantly higher for CEUS (85%) than for B-mode (27%). Mean intra-observer differences (95% limits of agreement) were 0.013 mL (0.803–4.097 mL) for Reader 1 and 0.031 mL (0.763–3.931 mL) for Reader 2 using CEUS, while they were 0.567 mL (-2.180–4.317 mL, Reader 1) and 0.759 mL (-2.584–4.290 mL, Reader 2) for B-mode. Intra-observer reproducibility was significantly higher for CEUS (96% and 95%, for the two readers) than for B-mode (21% and 23%). In conclusion, CEUS had higher reproducibility and inter- and intra-observer agreement compared to conventional B-mode in the assessment of radiofrequency-ablated volume of benign thyroid nodules.
Effects of Steam Sterilization on 3D Printed Biocompatible Resin Materials for Surgical Guides—An Accuracy Assessment Study
Authors: Sharma Neha , Cao Shuaishuai , Msallem Bilal , Kunz Christoph , Brantner Philipp , Honigmann Philipp , Thieringer Florian M.
Published: May 2020

Computer-assisted surgery with three-dimensional (3D) printed surgical guides provides more accurate results than free-hand surgery. Steam sterilization could be one of the factors that affect the dimensions of surgical guide resin materials, leading to inaccuracies during surgeries. The purpose of this study was to evaluate the effects of steam sterilization on the dimensional accuracy of indication-specific hollow cube test bodies, manufactured in-house using Class IIa biocompatible resin materials (proprietary and third-party). To evaluate the pre- and post-sterilization dimensional accuracy, root mean square (RMS) values were calculated. The results indicate that, in all the groups, steam sterilization resulted in an overall linear expansion of the photopolymeric resin material, with an increase in outer dimensions and a decrease in inner dimensions. The effects on the dimensional accuracy of test bodies were not statistically significant in all the groups, except PolyJet Glossy (p > 0.05). The overall pre- and post-sterilization RMS values were below 100 and 200 µm, respectively. The highest accuracies were seen in proprietary resin materials, i.e., PolyJet Glossy and SLA-LT, in pre- and post-sterilization measurements, respectively. The dimensional accuracy of third-party resin materials, i.e., SLA-Luxa and SLA-NextDent, were within a comparable range as proprietary materials and can serve as an economical alternative.
Trauma Can Induce Telangiectases in Hereditary Hemorrhagic Telangiectasia
Authors: Geisthoff Urban , Nguyen Ha-Long , Lefering Rolf , Maune Steffen , Thangavelu Kruthika , Droege Freya
Published: May 2020

Hereditary hemorrhagic telangiectasia (HHT) is an autosomal dominant disease of the fibrovascular tissue resulting in visceral vascular malformations and (muco-) cutaneous telangiectases with recurrent bleedings. The mechanism behind the disease is not fully understood; however, observations from HHT mouse models suggest that mechanical trauma may induce the formation of abnormal vessels. To assess the influence of environmental trauma (mechanical or light induced) on the number of telangiectases in patients with HHT, the number of telangiectases on the hands, face, and lips were counted on 103 HHT patients possessing at least three out of four Curaçao criteria. They were then surveyed for information concerning their dominant hand, exposure to sunlight, and types of regular manual work. Patients developed more telangiectases on their dominant hand and lower lip (Wilcoxon rank sum test: p < 0.001). Mechanical stress induced by manual work led to an increased number of telangiectases on patients’ hands (Mann–Whitney U test: p < 0.001). There was also a positive correlation between sun exposure and the number of telangiectases on the lips (Mann–Whitney U test: 0.027). This study shows that mechanical and UV-induced trauma strongly influence the formation of telangiectases in HHT patients. This result has potential implications in preventive measures and on therapeutic approaches for HHT.
Photodynamic Therapy Using Talaporfin Sodium for Local Failure after Chemoradiotherapy or Radiotherapy for Esophageal Cancer: A Single Center Experience
Authors: Ishida Natsuki , Osawa Satoshi , Miyazu Takahiro , Kaneko Masanao , Tamura Satoshi , Tani Shinya , Yamade Mihoko , Iwaizumi Moriya , Hamaya Yasushi , Furuta Takahisa , Sugimoto Ken
Published: May 2020

A phase II study of second-generation photodynamic therapy (PDT) using talaporfin sodium has shown excellent treatment results for esophageal cancer with local failure after chemoradiotherapy (CRT) or radiotherapy (RT). However, only a few studies have reported this therapy in clinical practice. This study aimed to confirm the efficacy and safety of salvage PDT using talaporfin sodium for esophageal cancer in various clinical situations. Twelve patients with esophageal cancer with local failure after definitive CRT or RT who underwent PDT using talaporfin sodium were enrolled from April 2016 to January 2020. Overall, 10 patients (83.3%) achieved a local complete response. No skin phototoxicity was observed, but esophageal stricture occurred in five patients (41.7%). Esophageal stricture was improved with endoscopic balloon dilation in all patients, and subsequent analysis found no significant factors causing esophageal stricture after PDT. Two patients with synchronous tumors were successfully rescued by combination therapy with endoscopic submucosal dissection. Two patients with carcinoma in situ of larger than 1/2 circumference were rescued by repeated PDT. The 2-year overall survival was 80.0% (95% confidence interval 0.409–0.946). PDT using talaporfin sodium was an effective and safe salvage treatment for esophageal cancer with local failure after CRT or RT in various clinical situations.
Imaging Modalities for the Diagnosis of Vascular Graft Infections: A Consensus Paper amongst Different Specialists
Authors: Lauri Chiara , Iezzi Roberto , Rossi Michele , Tinelli Giovanni , Sica Simona , Signore Alberto , Posa Alessandro , Tanzilli Alessandro , Panzera Chiara , Taurino Maurizio , Erba Paola Anna , Tshomba Yamume
Published: May 2020

Vascular graft infection (VGI) is a rare but severe complication of vascular surgery that is associated with a bad prognosis and high mortality rate. An accurate and prompt identification of the infection and its extent is crucial for the correct management of the patient. However, standardized diagnostic algorithms and a univocal consensus on the best strategy to reach a diagnosis still do not exist. This review aims to summarize different radiological and Nuclear Medicine (NM) modalities commonly adopted for the imaging of VGI. Moreover, we attempt to provide evidence-based answers to several practical questions raised by clinicians and surgeons when they approach imaging in order to plan the most appropriate radiological or NM examination for their patients.
Medication Management after Bariatric Surgery: Providing Optimal Patient Care
Authors: Porat Daniel , Dahan Arik
Published: May 2020

Substantially altered gastrointestinal anatomy/physiology after bariatric surgery presents new challenges for the proper medication management of these patients; drug absorption and bioavailability may increase, decrease, or remain unchanged post surgery, depending on the specific drug in question and the type of bariatric procedure. In this article, we offer a concise overview of the various aspects of this clinically significant issue, aiming to provide readers with a clear understanding as well as practical tools to handle drug management post bariatric surgery. Realizing the potentially altered pharmacokinetics of various drugs after bariatric surgery is essential for providing optimal pharmacological therapy and overall patient care.
Decline in Carbon Monoxide Transfer Coefficient in Chronic Obstructive Pulmonary Disease
Authors: Kim Yeon Wook , Lee Chang-Hoon , Hwang Hun-Gyu , Kim Yu-Il , Kim Deog-Kyeom , Oh Yeon-Mok , Lee Sang Haak , Kim Ki Uk , Lee Sang-Do
Published: May 2020

Background: Although a reduced carbon monoxide transfer coefficient (Kco) is an important feature in chronic obstructive pulmonary disease (COPD), how it changes over time and its relationship with other clinical outcomes remain unclear. This study evaluated longitudinal changes in Kco and their relationship with other clinical outcomes. Methods: We evaluated patients with COPD from the Korean Obstructive Lung Disease cohort, followed up for up to ten years. Random coefficient models were used to assess the annual change in Kco over time. Participants were categorized into tertiles according to Kco decline rate. Baseline characteristics and outcomes, including changes in FEV1 and emphysema index, incidence of exacerbations, and mortality, were compared between categories. Results: A decline in Kco was observed in 92.9% of the 211 enrolled participants with COPD. Those with the most rapid decline (tertile 1) had a lower FEV1/FVC% (tertile 1: 43.8% ± 9.7%, tertile 2: 46.4% ± 10.5%, tertile 3: 49.2% ± 10.4%, p = 0.008) and a higher emphysema index at baseline (27.7 ± 14.8, 22.4 ± 16.1, 18.1 ± 14.5, respectively, p = 0.001). Tertile 3 showed a lower decline rate in FEV1 (16.3 vs. 27.1 mL/yr, p = 0.017) and a lower incidence of exacerbations (incidence rate ratio = 0.66, 95% CI = 0.44–0.99) than tertile 1. There were no differences in the change in emphysema index and mortality between categories. Conclusion: Most patients with COPD experienced Kco decline over time, which was greater in patients with more severe airflow limitation and emphysema. Decline in Kco was associated with an accelerated decline in FEV1 and more frequent exacerbations; hence, this should be considered as an important outcome measure in further studies.
Mesenchymal Stem/Stromal Cells in Immunity and Disease: A Better Understanding for an Improved Use
Authors: Merimi Makram , Lagneaux Laurence , Agha Douâa Moussa , Lewalle Philippe , Meuleman Nathalie , Burny Arsène , Fahmi Hassan , Najar Mehdi
Published: May 2020

In this Special Issue, directed and supervised by Dr. Mehdi Najar, a collection of basic research articles and reviews, on the state of the art of Mesenchymal Stem/Stromal Cells (MSCs) immune biology, is presented. Among the major goals of this Special Issue is the presentation of an update about the immunomodulatory properties of MSCs and their capacity to respond to tissue microenvironment changes. MSCs hold great promise in the field of immunotherapy and regenerative medicine. Accordingly, a better understanding of MSC immune biology will improve their therapeutic value and use.
Chagas Disease: Detection of Trypanosoma cruzi by a New, High-Specific Real Time PCR
Authors: Kann Simone , Kunz Meik , Hansen Jessica , Sievertsen Jürgen , Crespo Jose J. , Loperena Aristides , Arriens Sandra , Dandekar Thomas
Published: May 2020

Background: Chagas disease (CD) is a major burden in Latin America, expanding also to non-endemic countries. A gold standard to detect the CD causing pathogen Trypanosoma cruzi is currently not available. Existing real time polymerase chain reactions (RT-PCRs) lack sensitivity and/or specificity. We present a new, highly specific RT-PCR for the diagnosis and monitoring of CD. Material and Methods: We analyzed 352 serum samples from Indigenous people living in high endemic CD areas of Colombia using three leading RT-PCRs (k-DNA-, TCZ-, 18S rRNA-PCR), the newly developed one (NDO-PCR), a Rapid Test/enzyme-linked immuno sorbent assay (ELISA), and immunofluorescence. Eighty-seven PCR-products were verified by sequence analysis after plasmid vector preparation. Results: The NDO-PCR showed the highest sensitivity (92.3%), specificity (100%), and accuracy (94.3%) for T. cruzi detection in the 87 sequenced samples. Sensitivities and specificities of the kDNA-PCR were 89.2%/22.7%, 20.5%/100% for TCZ-PCR, and 1.5%/100% for the 18S rRNA-PCR. The kDNA-PCR revealed a 77.3% false positive rate, mostly due to cross-reactions with T. rangeli (NDO-PCR 0%). TCZ- and 18S rRNA-PCR showed a false negative rate of 79.5% and 98.5% (NDO-PCR 7.7%), respectively. Conclusions: The NDO-PCR demonstrated the highest specificity, sensitivity, and accuracy compared to leading PCRs. Together with serologic tests, it can be considered as a reliable tool for CD detection and can improve CD management significantly.
Neuroimaging of Pediatric Intracerebral Hemorrhage
Authors: Sporns Peter B. , Psychogios Marios-Nikos , Fullerton Heather J. , Lee Sarah , Naggara Olivier , Boulouis Grégoire
Published: May 2020

Hemorrhagic strokes account for half of all strokes seen in children, and the etiologies of these hemorrhagic strokes differ greatly from those seen in adult patients. This review gives an overview about incidence and etiologies as well as presentation of children with intracerebral hemorrhage and with differential diagnoses in the emergency department. Most importantly it describes how neuroimaging of children with intracerebral hemorrhage should be tailored to specific situations and clinical contexts and recommends specific imaging protocols for acute and repeat imaging. In this context it is important to keep in mind the high prevalence of underlying vascular lesions and adapt the imaging protocol accordingly, meaning that vascular imaging plays a key role regardless of modality. Magnetic resonance imaging (MRI), including advanced sequences, should be favored whenever possible at the acute phase.
SARS-CoV-2 in Pregnancy: A Comprehensive Summary of Current Guidelines
Authors: Narang Kavita , Ibirogba Eniola R. , Elrefaei Amro , Trad Ayssa Teles Abrao , Theiler Regan , Nomura Roseli , Picone Olivier , Kilby Mark , Escuriet Ramón , Suy Anna , Carreras Elena , Tonni Gabriele , Ruano Rodrigo
Published: May 2020

Since the declaration of the global pandemic of COVID-19 by the World Health Organization on 11 March 2020, we have continued to see a steady rise in the number of patients infected by SARS-CoV-2. However, there is still very limited data on the course and outcomes of this serious infection in a vulnerable population of pregnant patients and their fetuses. International perinatal societies and institutions including SMFM, ACOG, RCOG, ISUOG, CDC, CNGOF, ISS/SIEOG, and CatSalut have released guidelines for the care of these patients. We aim to summarize these current guidelines in a comprehensive review for patients, healthcare workers, and healthcare institutions. We included 15 papers from 10 societies through a literature search of direct review of society’s websites and their journal publications up till 20 April 2020. Recommendations specific to antepartum, intrapartum, and postpartum were abstracted from the publications and summarized into Tables. The summary of guidelines for the management of COVID-19 in pregnancy across different perinatal societies is fairly consistent, with some variation in the strength of recommendations. It is important to recognize that these guidelines are frequently updated, as we continue to learn more about the course and impact of COVID-19 in pregnancy.
Comparative Safety of Bevacizumab, Ranibizumab, and Aflibercept for Treatment of Neovascular Age-Related Macular Degeneration (AMD): A Systematic Review and Network Meta-Analysis of Direct Comparative Studies
Authors: Plyukhova Anna A. , Budzinskaya Maria V. , Starostin Kirill M. , Rejdak Robert , Bucolo Claudio , Reibaldi Michele , Toro Mario D.
Published: May 2020

Background: Since the efficacy of ranibizumab (RBZ), bevacizumab (BVZ) and aflibercept (AFB) is comparable in neovascular age-related macular degeneration (AMD), we conducted a systematic review and meta-analysis to evaluate the long-term safety profiles of these agents, including ocular safety. Methods: Systematic review identifying randomized controlled trials (RCTs) comparing RBZ, BVZ and AFB directly published before March 2019. Serious ocular adverse events (SOAE) of special interest were endophthalmitis, pseudo-endophthalmitis, retinal pigment epithelium tear and newly identified macular atrophy. Results: Thirteen RCTs selected for meta-analysis (4952 patients, 8723 people-years follow-up): 10 compared RBZ vs. BVZ and three RBZ vs. AFB. There were no significant differences in almost all adverse events (systemic and ocular) between BVZ, RBZ and AFB in up to two years’ follow-up. Macular atrophy was reported heterogeneously and not reported as SOAE in most trials. Conclusions: Direct comparison of RBZ, BVZ and AFB safety profiles in the RCT network meta-analytical setting have not revealed a consistent benefit of these three commonly used anti-vascular endothelial growth factor (anti-VEGF) agents in AMD. Network model ranking highlighted potential benefits of RBZ in terms of a systemic safety profile; however, this appears a hypothesis rather than a conclusion. Newly identified macular atrophy is underestimated in RCTs—future real-world data should be focused on SOAE.
New Ways to Disinfect and Fill Dental Hard Tissues
Authors: Zehnder Matthias
Published: May 2020

The main biological goal in clinical dentistry remains to prevent micro-organisms from exerting pathogenic effects by establishing non-commensal biofilms or entering the wrong niches [...]
No Differences in Gastrointestinal Bleeding Risk among Clopidogrel-, Ticagrelor-, or Prasugrel-Based Dual Antiplatelet Therapy
Authors: Laredo Viviana , Sostres Carlos , García Sandra , Carrera-Lasfuentes Patricia , Revilla-Marti Pablo , Lanas Ángel
Published: May 2020

The risk for gastrointestinal bleeding from dual antiplatelet therapy (DAPT) with new antiplatelets (prasugrel/ticagrelor) compared to clopidogrel is unclear. Aim: To determine the risk and type of major (gastrointestinal bleeding requiring hospitalization) and minor (anemia and iron deficiency) gastrointestinal events with different types of DAPT. Methods: Retrospective observational cohort study of patients who started DAPT after percutaneous coronary intervention. Follow-up was censored after 12 months of DAPT, when a major gastrointestinal event occurred, or when DAPT was discontinued. Results: Among 1,327 patients (54.03% were treated with clopidogrel-based DAPT, 38.13% with ticagrelor-based DAPT, and 7.84% with prasugrel-based DAPT), 29.5% had at least one gastrointestinal event. Patients taking clopidogrel-DAPT were older, with more comorbidities, and higher gastrointestinal risk compared to those taking other DAPT regimens. Adjusted hazard ratios (HRs) showed no between-group differences in the risk for major (clopidogrel vs. new antiplatelets: HR 0.996; 95% confidence interval 0.497–1.996) and minor (HR 0.920; 0.712–1.189) gastrointestinal events. Most patients received proton pump inhibitors while on DAPT (93.3%) and after withdrawal (83.2%). Conclusion: Prasugrel- or ticagrelor-based DAPT was not associated with increased gastrointestinal bleeding risk when compared to clopidogrel-DAPT. New antiplatelets do not necessarily need to be restricted to patients with low gastrointestinal risk.
Viscous Damping Identification for a Wave Energy Converter Using CFD-URANS Simulations
Authors: Fontana Marco , Casalone Pietro , Sirigu Sergej Antonello , Giorgi Giuseppe , Bracco Giovanni , Mattiazzo Giuliana
Published: May 2020

During the optimization phase of a wave energy converter (WEC), it is essential to be able to rely on a model that is both fast and accurate. In this regard, Computational Fluid Dynamic (CFD) with Reynolds Averaged Navier–Stokes (RANS) approach is not suitable for optimization studies, given its computational cost, while methods based on potential theory are fast but not accurate enough. A good compromise can be found in boundary element methods (BEMs), based on potential theory, with the addition of non-linearities. This paper deals with the identification of viscous parameters to account for such non-linearities, based on CFD-Unsteady RANS (URANS) analysis. The work proposes two different methodologies to identify the viscous damping along the rotational degree of freedom (DOF) of pitch and roll: The first solely involves the outcomes of the CFD simulations, computing the viscous damping coefficients through the logarithmic decrement method, the second approach solves the Cummins’ equation of motion, via a Runge-Kutta scheme, selecting the damping coefficients that minimize the difference with CFD time series. The viscous damping is mostly linear for pitch and quadratic for roll, given the shape of the WEC analysed.
Potential Role of Laccases in the Relationship of the Maize Late Wilt Causal Agent, Magnaporthiopsis maydis, and Its Host
Authors: Degani Ofir , Goldblat Yuval
Published: May 2020

Late wilt is a vascular disease of maize (Zea mays L.) caused by the soil-borne and seed-borne fungus Magnaporthiopsis maydis. The pathogen penetrates the roots of maize plants at the seedling stage, grows into the xylem vessels, and gradually spreads upwards. From the flowering stage to the kernel ripening, the fungal hyphae and secreted materials block the water supply in susceptible maize cultivars, leading to rapid dehydration and death. Laccase is an enzyme secreted by fungus for diverse purposes. The M. maydis laccase gene was identified in our laboratory, but under what conditions it is expressed and to what functions remain unknown. In the current study, we tested the influence of plant age and tissue source (roots or leaves) on M. maydis laccase secretion. The results show increasing laccase secretion as corn parts (as ground tissue) were added to the minimal medium (MM). Furthermore, roots stimulated laccase secretion more than leaves, and adult plants enhanced laccase secretion more than young plants. This implies the possibility that the richer lignin tissue of adult plants may cause increased secretion of the enzyme. In vitro pathogenicity assay proved the ability of M. maydis to develop inside detached roots of maize, barley, watermelon, and cotton but not peanut. Testing root powder from those plants in MM revealed a negative correlation between M. maydis growth (expressed as biomass) and laccase secretion. For example, while the addition of maize, barley, or cotton root powder led to increasing fungal dry weight, it also resulted in relatively lower laccase activity. Watermelon and peanut root powder led to opposite responses. These findings suggest a pivotal role of laccase in the ability of M. maydis to exploit and grow on different host tissues. The results encourage further examination and a deeper understanding of the laccase role in these interesting host–pathogen interactions.
Coupling Analysis of Urban Land Use Benefits: A Case Study of Xiamen City
Authors: Ji Xuanming , Wang Kun , Ji Tao , Zhang Yihua , Wang Kun
Published: May 2020

The high coupling coordination of urban land use benefits is a significant factor for urbanization and sustainable urban development. This study, based on the statistical data from 2002 to 2017 of Xiamen City, constructs an index system that includes social, economic, ecological, and environmental benefits by evaluating the overall coupling coordination degree of land use benefits, using the entropy weight method (EWM), the coupling coordination degree (CCD) model, and the dynamic coupling coordination degree (DCCD) model. The results show that the coupling degree of Xiamen City’s land use is relatively low, while showing a positive trend of development. In terms of the management of land use, the market should play a major role to achieve more efficient land use and promote industrial upgrading. The government should take responsibility for supplying infrastructure, perfecting related laws and regulations, intervening the land use according to the law of markets, and expanding the investment in education, as well as science and technology.
Desertification–Scientific Versus Political Realities
Authors: Sterk Geert , Stoorvogel Jetse J.
Published: May 2020

Desertification is defined as land degradation occurring in the global drylands. It is one of the global problems targeted under the Sustainable Development Goals (SDG 15). The aim of this article is to review the history of desertification and to evaluate the scientific evidence for desertification spread and severity. First quantitative estimates of the global extent and severity of desertification were dramatic and resulted in the establishment of the UN Convention to Combat Desertification (UNCCD) in 1994. UNCCD’s task is to mitigate the negative impacts of desertification in drylands. Since the late 1990s, science has become increasingly critical towards the role of desertification in sustainable land use and food production. Many of the dramatic global assessments of desertification in the 1970s and 1980s were heavily criticized by scientists working in drylands. The used methodologies and the lack of ground-based evidence gave rise to critical reflections on desertification. Some even called desertification a myth. Later desertification assessments relied on remote sensing imagery and mapped vegetation changes in drylands. No examples of large areas completely degraded were found in the scientific literature. In science, desertification is now perceived as a local feature that certainly exists but is not as devastating as was earlier believed. However, the policy arena continues to stress the severity of the problem. Claims that millions of hectares of once productive land are annually lost due to desertification are regularly made. This highlights the disconnection between science and policy, and there is an urgent need for better dialogue in order to achieve SDG 15.
4-(3-Phenyl-1H-pyrazolo[3,4-d]pyrimidin-1-yl)thieno[3,2-d]pyrimidine
Authors: Noh Eun Sun , Kim Sung Min , Song Yang-Heon
Published: May 2020

A new hybrid compound, 4-(3-phenyl-1H-pyrazolo[3,4-d]pyrimidin-1-yl)thieno[3,2-d]pyrimidine 3, with promising biological activity was efficiently synthesized by the reaction of 3-phenyl-1-(thieno[3,2-d]pyrimidin-4-yl)-1H-pyrazol-5-amine with Vilsmeier–Haack reagent and subsequent treatment with ammonium carbonate. The structure of the synthesized compound was fully characterized by 1H-, 13C-NMR, IR spectroscopy, mass-spectrometry and elemental analysis.
Experimental Analysis of Space Trusses Using Spacers of Concrete with Steel Fiber and Sisal Fiber
Authors: Silva Welington V. , Silva Ramon , Bezerra Luciano M. , Freitas Cleirton A. S. , Bonilla Jorge
Published: May 2020

Space trusses are structural systems, generally made of tubes, used worldwide because of their advantages in covering long-span roofs. In addition to having a low cost, the truss weight is relatively reduced. The load capacity of these structures depends also on the strength of their node connection. Connections made with the superposition of flattened tube ends trespassed by one bolt are, generally, known as typical nodes. They are inexpensive but present eccentricities that reduce significantly the strength of such space trusses. To increase the truss load capacity, this research presents the results of an experimental program to reduce the eccentricities of the typical nodes. This reduction is done with a new type of spacer made of encapsulated concrete with steel fiber or sisal fiber. The experimental tests showed that the trusses with typical nodes collapsed under reduced load by local failure due to high distortions at the nodes. The trusses with encapsulated concrete spacer showed good results, with an increase in collapse load of 36% and failure by buckling bars.
Hydrogen Permeation in X65 Steel under Cyclic Loading
Authors: Cabrini Marina , Coppola Luigi , Lorenzi Sergio , Testa Cristian , Carugo Francesco , Bucella Diego Pesenti , Pastore Tommaso
Published: May 2020

This experimental work analyzes the hydrogen embrittlement mechanism in quenched and tempered low-alloyed steels. Experimental tests were performed to study hydrogen diffusion under applied cyclic loading. The permeation curves were fitted by considering literature models in order to evaluate the role of trapping—both reversible and irreversible—on the diffusion mechanism. Under loading conditions, a marked shift to the right of the permeation curves was noticed mainly at values exceeding the tensile yield stress. In the presence of a relevant plastic strain, the curve changes due to the presence of irreversible traps, which efficiently subtract diffusible atomic hydrogen. A significant reduction in the apparent diffusion coefficient and a considerable increase in the number of traps were noticed as the maximum load exceeded the yield strength. Cyclic loading at a tensile stress slightly higher than the yield strength of the material increases the hydrogen entrapment phenomena. The tensile stress causes a marked and instant reduction in the concentration of mobile hydrogen within the metal lattice from 55% of the yield strength, and it increases significantly in the plastic field.
Laser-Based Ablation of Titanium–Graphite Composite for Dental Application
Authors: Šugár Peter , Ludrovcová Barbora , Kováčik Jaroslav , Sahul Martin , Šugárová Jana
Published: May 2020

Biocompatible materials with excellent mechanical properties as well as sophisticated surface morphology and chemistry are required to satisfy the requirements of modern dental implantology. In the study described in this article, an industrial-grade fibre nanosecond laser working at 1064 nm wavelength was used to micromachine a new type of a biocompatible material, Ti-graphite composite prepared by vacuum low-temperature extrusion of hydrogenated-dehydrogenated (HDH) titanium powder mixed with graphite flakes. The effect of the total laser energy delivered to the material per area on the machined surface morphology, roughness, surface element composition and phases transformations was investigated and evaluated by means of scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), confocal laser-scanning microscopy (CLSM) and X-ray diffraction analysis (XRD). The findings illustrate that the amount of thermal energy put to the working material has a remarkable effect on the machined surface properties, which is discussed from the aspect of the contact properties of dental implants.
Stochastic Memristive Quaternion-Valued Neural Networks with Time Delays: An Analysis on Mean Square Exponential Input-to-State Stability
Authors: Humphries Usa , Rajchakit Grienggrai , Kaewmesri Pramet , Chanthorn Pharunyou , Sriraman Ramalingam , Samidurai Rajendran , Lim Chee Peng
Published: May 2020

In this paper, we study the mean-square exponential input-to-state stability (exp-ISS) problem for a new class of neural network (NN) models, i.e., continuous-time stochastic memristive quaternion-valued neural networks (SMQVNNs) with time delays. Firstly, in order to overcome the difficulties posed by non-commutative quaternion multiplication, we decompose the original SMQVNNs into four real-valued models. Secondly, by constructing suitable Lyapunov functional and applying It o ^ ’s formula, Dynkin’s formula as well as inequity techniques, we prove that the considered system model is mean-square exp-ISS. In comparison with the conventional research on stability, we derive a new mean-square exp-ISS criterion for SMQVNNs. The results obtained in this paper are the general case of previously known results in complex and real fields. Finally, a numerical example has been provided to show the effectiveness of the obtained theoretical results.
From Grammar Inference to Semantic Inference—An Evolutionary Approach
Authors: Kovačević Željko , Mernik Marjan , Ravber Miha , Črepinšek Matej
Published: May 2020

This paper describes a research work on Semantic Inference, which can be regarded as an extension of Grammar Inference. The main task of Grammar Inference is to induce a grammatical structure from a set of positive samples (programs), which can sometimes also be accompanied by a set of negative samples. Successfully applying Grammar Inference can result only in identifying the correct syntax of a language. With the Semantic Inference a further step is realised, namely, towards inducing language semantics. When syntax and semantics can be inferred, a complete compiler/interpreter can be generated solely from samples. In this work Evolutionary Computation was employed to explore and exploit the enormous search space that appears in Semantic Inference. For the purpose of this research work the tool LISA.SI has been developed on the top of the compiler/interpreter generator tool LISA. The first results are encouraging, since we were able to infer the semantics only from samples and their associated meanings for several simple languages, including the Robot language.
Relaxed Inertial Tseng’s Type Method for Solving the Inclusion Problem with Application to Image Restoration
Authors: Abubakar Jamilu , Kumam Poom , Hassan Ibrahim Abdulkarim , Padcharoen Anantachai
Published: May 2020

The relaxed inertial Tseng-type method for solving the inclusion problem involving a maximally monotone mapping and a monotone mapping is proposed in this article. The study modifies the Tseng forward-backward forward splitting method by using both the relaxation parameter, as well as the inertial extrapolation step. The proposed method follows from time explicit discretization of a dynamical system. A weak convergence of the iterates generated by the method involving monotone operators is given. Moreover, the iterative scheme uses a variable step size, which does not depend on the Lipschitz constant of the underlying operator given by a simple updating rule. Furthermore, the proposed algorithm is modified and used to derive a scheme for solving a split feasibility problem. The proposed schemes are used in solving the image deblurring problem to illustrate the applicability of the proposed methods in comparison with the existing state-of-the-art methods.
Sepsis Associated Delirium
Authors: Atterton Ben , Paulino Maria Carolina , Povoa Pedro , Martin-Loeches Ignacio
Published: May 2020

Sepsis is a potentially life-threatening condition caused by a systemic dysregulated host response to infection. The brain is particularly susceptible to the effects of sepsis with clinical manifestations ranging from mild confusion to a deep comatose state. Sepsis-associated delirium (SAD) is a cerebral manifestation commonly occurring in patients with sepsis and is thought to occur due to a combination of neuroinflammation and disturbances in cerebral perfusion, the blood brain barrier (BBB) and neurotransmission. The neurological impairment associated with SAD can persist for months or even longer, after the initial septic episode has subsided which may impair the rehabilitation potential of sepsis survivors. Early identification and treatment of the underlying sepsis is key in the management of SAD as once present it can be difficult to control. Through the regular use of validated screening tools for delirium, cases of SAD can be identified early; this allows potentially aggravating factors to be addressed promptly. The usefulness of biomarkers, neuroimaging and electroencephalopathy (EEG) in the diagnosis of SAD remains controversial. The Society of Critical Care Medicine (SCCM) guidelines advise against the use of medications to treat delirium unless distressing symptoms are present or it is hindering the patient’s ability to wean from organ support.
Effect of Surface Roughness on Pitting Corrosion of AZ31 Mg Alloy
Authors: Mitchell Jeffrey , Crow Natalie , Nieto Andy
Published: May 2020

Magnesium is a highly desired material for multiple applications including automotive, aircraft, and biomedical components due to its high strength to weight ratio, non-toxicity, and its good machinability. The main drawback of magnesium use is its poor resistance to corrosion. The goal of this experiment was to determine the effect of surface roughening treatments on the rate of corrosion and to characterize the resultant magnesium oxide (MgO) and brucite (Mg(OH)2) corrosion products. Three samples of AZ31 alloy with varying initial surface roughness were exposed to simulated marine conditions using a salt fog chamber for 672 h. The salt fog experiments used a 3.5 wt.% NaCl solution and were conducted in accordance with the ASTM B117 test protocols. One sample was tested in an as-received state, served as the control, another sample was roughened using a grit blaster, and another was polished to a mirror finish. Formed oxide scales were characterized using scanning electron microscopy, X-ray diffraction, and energy dispersive spectroscopy. The samples with a higher roughness underwent the severest corrosion and ensuing formation of the thickest oxide layers. The results indicate that the initial surface roughness has a profound effect on the resulting severity of pitting corrosion in Mg alloys and that mechanical polishing can substantially reduce the propensity for uniform and pitting corrosion to occur.
Modulatory Effect of Nicotinic Acid on the Metabolism of Caco-2 Cells Exposed to IL-1β and LPS
Authors: Santoru Maria Laura , Piras Cristina , Murgia Federica , Spada Martina , Tronci Laura , Leoni Vera Piera , Serreli Gabriele , Deiana Monica , Atzori Luigi
Published: May 2020

Inflammatory bowel diseases (IBD) are the most common gastrointestinal inflammatory pathologies. Previous work evidenced a lower content of nicotinic acid (NA) in feces of IBD patients compared to healthy subjects. In the present study, we aimed to understand the effects of NA on intestinal inflammation, as several studies reported its possible beneficial effect, and investigate its influence on inflammation-driven metabolism. NA was tested on a Caco-2 in-vitro model in which inflammation was induced with interleukin-1β (IL-1β) and lipopolysaccharide (LPS), two mayor proinflammatory compounds produced in IBD, that stimulate the production of cytokines, such as interleukin 8. A metabolomics approach, with gas chromatography–mass spectrometry (GC-MS) and nuclear proton magnetic resonance (1H-NMR), was applied to study the metabolic changes. The results showed that NA significantly reduced the level of IL-8 produced in both LPS and IL-1β stimulated cells, confirming the anti-inflammatory effect of NA also on intestinal inflammation. Moreover, it was demonstrated that NA treatment had a restoring effect on several metabolites whose levels were modified by treatments with IL-1β or LPS. This study points out a possible use of NA as anti-inflammatory compound and might be considered as a promising starting point in understanding the beneficial effect of NA in IBD.
Cross-Omics: Integrating Genomics with Metabolomics in Clinical Diagnostics
Authors: Kerkhofs Marten H. P. M. , Haijes Hanneke A. , Willemsen A. Marcel , van Gassen Koen L. I. , van der Ham Maria , Gerrits Johan , de Sain-van der Velden Monique G. M. , Prinsen Hubertus C. M. T. , van Deutekom Hanneke W. M. , van Hasselt Peter M. , Verhoeven-Duif Nanda M. , Jans Judith J. M.
Published: May 2020

Next-generation sequencing and next-generation metabolic screening are, independently, increasingly applied in clinical diagnostics of inborn errors of metabolism (IEM). Integrated into a single bioinformatic method, these two –omics technologies can potentially further improve the diagnostic yield for IEM. Here, we present cross-omics: a method that uses untargeted metabolomics results of patient’s dried blood spots (DBSs), indicated by Z-scores and mapped onto human metabolic pathways, to prioritize potentially affected genes. We demonstrate the optimization of three parameters: (1) maximum distance to the primary reaction of the affected protein, (2) an extension stringency threshold reflecting in how many reactions a metabolite can participate, to be able to extend the metabolite set associated with a certain gene, and (3) a biochemical stringency threshold reflecting paired Z-score thresholds for untargeted metabolomics results. Patients with known IEMs were included. We performed untargeted metabolomics on 168 DBSs of 97 patients with 46 different disease-causing genes, and we simulated their whole-exome sequencing results in silico. We showed that for accurate prioritization of disease-causing genes in IEM, it is essential to take into account not only the primary reaction of the affected protein but a larger network of potentially affected metabolites, multiple steps away from the primary reaction.
Antimicrobial Resistance Traits of Escherichia coli Isolated from Dairy Manure and Freshwater Ecosystems Are Similar to One Another but Differ from Associated Clinical Isolates
Authors: Beattie Rachelle E. , Bakke Ellen , Konopek Nicholas , Thill Rebecca , Munson Erik , Hristova Krassimira R.
Published: May 2020

Antimicrobial resistance (AMR) is a prevalent global health problem across human and veterinary medicine. The One Health approach to AMR is necessary to mitigate transmission between sources of resistance and decrease the spread of resistant bacteria among humans, animals, and the environment. Our primary goal was to identify associations in resistance traits between Escherichia coli isolated from clinical (n = 103), dairy manure (n = 65), and freshwater ecosystem (n = 64) environments within the same geographic location and timeframe. Clinical E. coli isolates showed the most phenotypic resistance (47.5%), followed by environmental isolates (15.6%) and manure isolates (7.7%), with the most common resistances to ampicillin, ampicillin-sulbactam, and cefotaxime antibiotics. An isolate subset was screened for extended spectrum beta-lactamase (ESBL) production resulting in the identification of 35 ESBL producers. The most common ESBL gene identified was blaTEM-1. Additionally, we found nine different plasmid replicon types including IncFIA-FIB, which were frequently associated with ESBL producer isolates. Molecular phylotyping revealed a significant portion of clinical E. coli were associated with phylotype B2, whereas manure and environmental isolates were more diverse. Manure and environmental isolates were significantly different from clinical isolates based on analyzed traits, suggesting more transmission occurs between these two sources in the sampled environment.
The Threat of Antibiotic Resistance in Changing Climate
Authors: Fouladkhah Aliyar Cyrus , Thompson Brian , Camp Janey Smith
Published: May 2020

As the earliest form of life, microorganisms have elaborate mechanisms for adapting to changes in environmental conditions [...]
Diversity, Transmission, and Cophylogeny of Ledanteviruses (Rhabdoviridae: Ledantevirus) and Nycteribiid Bat Flies Parasitizing Angolan Soft-Furred Fruit Bats in Bundibugyo District, Uganda
Authors: Bennett Andrew J. , Paskey Adrian C. , Kuhn Jens H. , Bishop-Lilly Kimberly A. , Goldberg Tony L.
Published: May 2020

Obligate hematophagous ectoparasitic flies of the superfamily Hippoboscoidea are distributed worldwide, but their role as vectors and reservoirs of viruses remains understudied. We examined hippoboscoid bat flies (family Nycteribiidae) parasitizing Angolan soft-furred fruit bats (Lissonycteris angolensis ruwenzorii) from Bundibugyo District, Uganda. Using metagenomic methods, we detected 21 variants of the rhabdovirid genus Ledantevirus, which contains medically important “bat-associated” viruses. These 21 viruses, representing at least two divergent viral lineages, infected 26 bat flies from 8 bats in a single roost. Cophylogenetic analyses of viruses and bat flies resulted in strong evidence of virus-host codivergence, indicating vertical transmission of bat fly ledanteviruses. Examination of oral swabs from bats revealed ledantevirus RNA in the saliva of 1 out of 11 bats, with no evidence of insect genetic material in the mouth of this bat. These data demonstrate that bat flies can harbor diverse ledanteviruses even in a single roost and that the predominant mode of transmission is likely vertical (among bat flies), but that bats can become infected and shed viruses orally. In conclusion, bat flies may serve as ectoparasitic reservoirs of “bat-associated” viruses that only transiently or sporadically infect bats.
Association between Gut Microbiota and Infant’s Temperament in the First Year of Life in a Chinese Birth Cohort
Authors: Wang Ying , Chen Xiaoli , Yu Yun , Liu Yanqun , Zhang Qing , Bai Jinbing
Published: May 2020

Infant temperament characteristics play a critical role in children’s developmental pathways and can predict adulthood psychopathology. The diversity and composition of the gut microbiota are associated with human temperament in both adults and young children. However, the relationship between the gut microbiota and temperament in 12-month-old infants is rarely studied; this developmental period is when temperament reaches a relatively stable stage. We used high-throughput sequencing methods to explore whether temperament characteristics were associated with gut microbiota diversity and composition. Infants’ fecal samples were collected at 12 months of age for the gut microbiota analysis. Based on the primary caregivers’ reports, infants’ temperaments were measured using the Infant Behavior Questionnaire-revised (IBQ-R). This study included 51 infants, including 20 boys and 31 girls, with a mean age of 12.25 months. Results showed that soothability was positively correlated with maternal education level (β = 0.29, p = 0.043, adjust p = 0.025) and the abundance of Bifidobacterium genera (β = 0.62, p = 0.004, adjust p = 0.002). Conversely, cuddliness was negatively correlated with the abundance of Hungatella genera. There was no significant difference in temperament based on gender. This study demonstrated that gut microbiota composition was associated with temperament in 12-month-old infants. These results point to the importance of gut microbiota balance. Future studies on the mechanisms behind the gut microbiota affecting temperament are warranted.