bluuebunny/crossref_metadata_2025 huggingface dataset:
Hematological Changes Associated with Pregnancy in Domestic Sows (Suis domesticus)
Authors: Okafor Simeon C. , Chukwudi Chinwe U. , Okanya Kevin C. , Ihedioha John I.
Published: June 2021

Background: Pregnancy involves complex biological interaction and communication between the developing conceptus and maternal uterus, which may alter the blood cellular elements. The present investigation evaluated the hematological changes associated with pregnancy in domestic sows. Methods: Blood samples were collected by auricular venipuncture from twenty sexually mature domestic sows from four pig farms. Hematological analyses were carried out on the blood samples following standard procedures. Result: The mean packed cell volume (PCV), the red blood cell (RBC) count, hemoglobin (Hb) concentration, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and absolute lymphocyte count were higher during pregnancy and lower following parturition, whereas, the mean corpuscular volume (MCV) was lower during pregnancy and declined further following parturition. The mean total white blood cell (TWBC) count and the absolute neutrophil count were higher during pregnancy and further elevated postpartum while the absolute monocyte count was lower during pregnancy and elevated postpartum. The absolute eosinophil count was significantly (p less than 0.05) lower in the pregnant sows (0.35 ± 0.13) and postpartum sows (0.21 ± 0.15) than in the non-pregnant sows (1.06 ± 0.27). These hematological alterations could assist in monitoring the physiological and health status of the dam, fetuses and pregnancy diagnosis.
Livestock Breeding for Disease Resistance: A Perspective Review
Authors: Gogoi Ankita , Das Bula , Chabukdhara Prasanta , Phookan Arundhati , Phangchopi Donna
Published: June 2021

Livestock plays a momentous role in a country’s economy especially in a developing nation like India, where livelihoods of about 20.5 million people depend upon livestock and its allied sectors, suggesting that about two-third of the rural community is involved (Annual Report, 2018). The small ruminants like sheep and goat provide main source of income for many economically poor people, especially marginal and landless farmers. Similarly the role of these livestock to various pastoral communities cannot be ignored. So whenever there is a disease outbreak, the burden of huge economic loss falls on these poor livestock keepers. The traditional methods of disease management involve either treatment with antibiotics or vaccination for prevention of diseases. However, the indiscriminate use of these antibiotics has lead to antibiotic-resistant pathogens and likewise it is extremely difficult to develop vaccines against a wide range of pathogens that are frequently mutating and are caused by multi etiological factors. Hence, considering all these factors breeding of disease resistant animals becomes a top priority in this changing world. The literature is filled with references of indigenous animals and poultry being resistant to many diseases. Keeping this in view, the current review has been written to highlight sustainable and feasible methods for breeding them for disease resistance.
Social minimum living standard in Russia: estimation methods
Authors: Migranova Lyudmila , Korchagina Irina
Published: June 2021

The minimum living standard of the population in Russia since 1992 is subsistence minimum (SM). It plays a significant social role as a criterion for the assessment of the need of households for social assistance. The methods for SM calculating were periodically changed, but the rate of absolute poverty was always measured by the share of the population with income below SM. The article considers in detail the methods for estimating SM, adopted in 2013 and based on normative-statistical approach. Analysis of the food consumption and the structure of consumer spending of poor households, based on Household Budget Survey 2012-2019, revealed their non-compliance with the SM that was effective up to and including 2020. Since 2021 estimation of poverty is made by statistical methods without calculating minimum consumer basket (Law № 473-ФЗ as of 29.12.2020). The poverty line is determined by the ratio to median income per capita, and the poverty rate is of relative character. Retrospective analysis of the dynamics in the per capita and median incomes of the RF population and the SM per capita for 2013-2020 makes it possible to estimate the difference in the rates of absolute and relative poverty and to understand what guided the developers of the law in determining the SM per capita at 44.2% of the median income and the value of SM for the main sociodemographic groups of the population for the year 2021. In addition, on concrete examples, by means of the logarithmic normal function used by Rosstat for calculation of the population income distribution, it is shown, at what ratio of the median income to the average, the rate of absolute poverty may be reduced by half as compared to 2019
FUNGAL KERATITIS WITH CORNEAL ULCER IN FARMER
Authors: Cahyana Nugraha Wahyu
Published: June 2021

Fungal keratitis is an important cause of corneal blindness all over the world, especially in developing countries. Fungal Keratitis can diagnosis by slit-lamp biomicroscopic examination and culture is essential for early specific diagnosis and must be taken into consideration to establish the most effective treatment and avoid severe complications. The study was present a case of Fungal Keratitis in Farmer with a corneal ulcer caused by rice seeds corneal corpus allienum. A corneal ulcer is a complication was caused by ineffective therapy especially Steroid topical that should be avoided. The culture test result was C. Albicans and was treated with natamycin 5% Eye drops. Finally, patients have a good clinical response, however with the sequel of decreased visual acuity.
The Financing of the University of Rome (15th-16th centuries)
Authors: Frova Carla
Published: June 2021

Se aprecia en los últimos años un interés creciente en la historiografía por las universidades que se desarrollaron en territorios de la Iglesia Romana a finales de la Edad Media. Si bien podemos afirmar que existieron universidades papales en la edad moderna no fue así en la Edad Media. Los trabajos sobre la universidad de Perugia, también sobre la universidad de Roma, dejan ver el dualismo entre ciudad y curia, entre el trabajo académico y la tutela papal, entre la gestión municipal, la vida de la ciudad y la presencia de la iglesia. Y, si nos centramos en la financiación de la Universidad de Roma, también surgen cuestiones relacionadas con el papel de los gobiernos municipales en la determinación de las políticas financieras de la universidad. Este artículo abordará la financiación del Studium Urbis por parte de las autoridades públicas para observar la capacidad de gestión de la misma en las coordenadas específicas y espaciales de la Universidad de Roma.
Francisco Javier Rubio Muñoz. La República de sabios. Profesores, cátedras y universidad en la Salamanca del siglo de Oro.
Authors: Ramis Barceló Rafael
Published: June 2021

Este artículo reseña: Francisco Javier Rubio Muñoz. La  República de sabios. Profesores, cátedras y universidad en la Salamanca del  siglo de Oro. Madrid: Dykinson, 2020. 289 pp.
Governing Complexity in World Politics
Authors: Haas Peter M , Western Jon
Published: June 2021

Complexity is the new global ontology for world politics. This article summarizes the characteristics of complexity and its implications for informed US state policy making. We conclude with some suggestions about administrative reforms to improve US policy making to address global complexity.
Saúde, ciência e política na encruzilhada de discursos
Authors: Silva Luiz Felipe Andrade , Baalbaki Angela Corrêa Ferreira
Published: June 2021

Este artigo tem como objetivo analisar o discurso sobre vacinação contra o novo coronavírus. Fundamenta-se no quadro teórico da Análise do Discurso materialista e toma como corpus principal textualidades digitais contemporâneas que circulam no Twitter, observando para tal o modo de funcionamento das marcas linguísticas presentes nas textualidades selecionadas. Uma guerra (informacional) da vacina está sendo produzida: sentidos favoráveis e contrários circulam e fazem ecoar efeitos de sentidos que percorreram outros momentos históricos por meio de um trabalho da memória discursiva. Em virtude desse movimento de sentidos, constituímos um corpus secundário com charges publicadas em jornais do início do século XX e imagens postadas em outra rede social, o Facebook. Trabalhando no entremeio de diferentes textualidades, promovemos um gesto de interpretação que intenta a constituição do discurso anticiência, na inter-relação com o discurso conservador.
Etawa Goat Estrus Quality with Estrus Synchronization
Authors: Pangestuningrum Jihan , Madyawati Sri Pantja , Eliyani Hana , Damayanti Ratna , Rochmi Siti Eliana
Published: June 2021

Background: Estrus synchronization is a way to get a herd of cattle estrus at the same time. This method can increase the reproductive success of livestock, thereby increasing production and population. Purpose: To determine the estrus quality from estrus synchronized Boerja goats, in Trawas District, Mojokerto Regency. Method: Estrus synchronization using an intra-vaginal sponge contains hormone progesterone, combined with the injection of the hormone GnRH. an intra-vaginal progesterone sponge was inserted for 14 days. After 14 days, the goats were injected with the GnRH hormone, then placed in a cage with the male and observed for estrus quality. Result: the average onset of estrus was 40.6 hours, the average color of the goat's vulvar mucosa when estrus was 2.3 (dark pink), the average presence of mucus was 1.9 (excess vulvar mucus), the average mucus color is 2.8 (white), and the average temperature increase of the vulva when the goat is in estrus is 0.4 ° C. Conclusion: The observations showed that the estrus quality of the goats was very diverse and not all goats were able to show the maximum estrus quality. It can be due to genetic factors, body condition, and feed nutrition.
ANEMIA OF PREGNANCY ASSESSED FROM THE COMPLIANCE BEHAVIOR OF PREGNANT WOMEN IN CONSUMING IRON SUPPLEMENTS IN THE WORKING AREA OF PITU HEALTH CENTER, NGAWI DISTRICT
Authors: Kurniasih Erwin
Published: June 2021

Introduction: pregnant women with anemia are at high risk of bleeding which can cause serious complications to both the mother and the fetus. In Indonesia, maternal mortality due to bleeding due to anemia is quite high because many pregnant women disobidient to consume iron supplements. The study was to determine the relationship between the obedience behavior of pregnant women in consuming iron supplements with the incidence of pregnancy anemia in the working area of the Pitu Community Health Center, Ngawi Regency.  Methods: This study used a cross sectional design and total sampling of 60 respondents. The data collection method was using a questionnaire and analyzed by using the Chi Squre test. Results: The results showed 18 respondents (30%) were at a high level of adherence with anemia condition 5 people (8.3%). Respondents with a moderate level of adherence were 34 people (56.7%) with anemia conditions 16 people (26.7%), and 8 respondents (13.3%) had a low level of compliance with anemia.  The statistical test results obtained p value = 0.003 (p <0.05). Conclusion: There is a relationship between compliance behavior of pregnant women in consuming Fe supplements with the incidence of pregnancy anemia
RELATIONSHIP LEVEL OF PARENTAL KNOWLEDGE ABOUT THE FUNCTION OF PLAYING WITH THE PROVISION OF APE (EDUCATIONAL GAME TOOLS) CHILDREN AGED 4-6 YEARS IN BALONG TUNJUNG VILLAGE, BENJENG DISTRICT, GRESIK
Authors: Sulpat Emuliana , Listianingsih Fitria
Published: June 2021

Introduction: Play is an activity where the child can perform or practice skills, express thoughts, be creative, prepare to play and behave maturely, the results of a preliminary study in Benjeng 50 parental (64.70%) do not know the function of play for children. The research objective is to determine the relationship between the level of parental knowledge and the provision of APE. Methods: The design of this study was a correlation study with a Cross Sectional approach, with all parent populations of 34 respondents, a sample of 20 respondents.  Sampling using simple random sampling technique, data taken using questionnaire and observation sheets, then analyzed by spearman test with a significance level a α = 0.05. Results: The results showed that (50%) of respondents had a moderate level of knowledge about the function of play and (65%) respondents gave APE. The statistical test result obtained rs = 0,499 and p= 0,025, where p> 0,05. Conclusion: This study is a relationship between the level of knowledge of parents about the function of play and the provision of APE,  in Balong Tunjung Village, Benjeng  , Gresik.
THE RELATIONSHIP OF KNOWLEDGE, ATTITUDE AND FAMILY SUPPORT WITH THE NUTRITIONAL STATUS OF THE ELDERLY: LITERATURE REVIEW
Authors: Ambrosia Barek Maria , Haryanto Joni , Ulfiana Elida
Published: June 2021

Introduction: Elderly are individuals who are vulnerable and at risk of experiencing health problems related to nutritional status. Families who live together are expected to be able to provide nutritional intake in accordance with the condition of the elderly so that adequate knowledge is needed in preparing the need for balanced nutrition. The purpose of this study was to analyze the relationship between knowledge, attitudes, and family support to elderly's nutritional status. Methods: The search for literature review articles was carried out in four databases (Scopus, Science Direct, Pub med, and Google Scholar) with a publication span of 2016-2020. The prism checklist was used to guide this review. Analysis and data tabulation were carried out in the article. Title, abstract, full text and methodology were assessed to determine study eligibility. Results: A total of 10 studies matched the inclusion criteria of the study, consisting of 6 cross-sectional studies and 4 qualitative studies. A review study found that good knowledge, positive attitude and good family support are directly proportional to the nutritional status of the elderly. Discussion: Family has a role in maintaining the health of the elderly, one of which is in fulfilling nutrition. Knowledge, attitudes and family support are related to the nutritional status of the elderly. Conclusions: Families who have knowledge related to nutrition can provide complete food intake and according to the needs of the elderly. A good family attitude will increase their support in helping fulfill nutrition.
Kedudukan Kreditor yang Tidak Terdaftar Pada Putusan Perdamaian PKPU Dalam Mengajukan Permohonan Pernyataan Pailit
Authors: Barnini Adam , Suryanti Nyulistiowati , Artaji Artaji
Published: June 2021

This article will examine the position of creditors who are not registered in the peace agreement that has been ratified in filing a bankruptcy statement and the strength of the homologation decision against creditors who are not registered in the peace agreement. The research method used is juridical normative by referring to written legal materials by reviewing secondary data in the form of primary, secondary and tertiary legal materials relating to bankruptcy and suspension of payment. The results of this study conclude that first, creditors who feel disadvantaged because they are not registered in the peace agreement cannot file a bankruptcy statement. Second, the homologation decision still has legal force against creditors who are not registered in the homologation decision.Keywords: Suspension Of Obligation For Payment Of Debt; Homologation Decision; Creditors are Not Registered in the Peace Agreement.Penelitian ini akan membahas mengenai kedudukan kreditor yang tidak terdaftar dalam perjanjian perdamaian yang telah disahkan dalam mengajukan permohonan pernyataan pailit dan kekuatan putusan homologasi terhadap kreditor yang tidak terdaftar dalam perjanjian perdamaian. Metode penelitian yang digunakan adalah yuridis normatif dengan merujuk pada bahan-bahan hukum tertulis dengan mengkaji data sekunder yang berupa bahan hukum primer, sekunder dan tersier yang berkaitan dengan kepailitan dan PKPU. Hasil penelitian ini menyimpulkan bahwa pertama, terhadap kreditor yang merasa dirugikan karena tidak terdaftar dalam perjanjian perdamaian tidak dapat mengajukan permohonan pernyataan pailit. Kedua, putusan homologasi tetap memiliki kekuatan hukum terhadap kreditor yang tidak terdaftar dalam putusan homologasi.Kata Kunci: PKPU; Putusan Homologasi; Kreditor Tidak Terdaftar; Perjanjian Perdamaian.
PERGESERAN LEGITIMASI BALIAN DALAM LOCAL INDIGENOUS GOVERNANCE DI DESA HINAS KANAN
Authors: Rezani Muhammad , Salim Ferdinan , Fijaini Fijaini
Published: June 2021

Balian used to be a Customary Institution that organizes all life of the village community in the Meratus Mountains, including managing the village by upholding the local wisdom. Since the New Order, the paradigm of “the state-enters-the village” governs villages through its set of legislation in the governmentality scheme. Behind the shield of will to improve (KAT empowerment) as a media of discursive power (Li, 2007), the government reshape all social order and local indigenous social governance to “obey” to one formal rule. Unconsciously or intentionally, that governmentality has triggered a shift in local indigenous governance from the basis of local wisdom to the basis of modern governance, with the risk of a shift in the legitimacy of Balian’s power vis-à-vis its community. This study is built on Foucault’ theory of governmentality (1982) and Li’s will to improve (2007). The research method used in this study was a qualitative descriptive method by collecting data, in-depth interviews, and participatory observation. The shift led the role and function of the Balian to be no longer the sole holder of local governance with a local wisdom approach. In the current local governance, it is held by the Village Government, the Sub-District Customary and Balian’s Heads. The shifts that also occurred in the community included the shifting of traditional culture in selling rice which was once considered a taboo to be a commonplace thing and the tendency of the community to resolve conflicts with government institutions rather than the customary or Balian’s Heads.
PELAYANAN PEMBAHARUAN E-KTP PADA DINAS KEPENDUDUKAN DAN PENCATATAN SIPIL KOTA BANJARMASIN
Authors: Hadiyanor Enly
Published: June 2021

In an effort to fulfill the obligation as a good Indonesian citizen of the city of Banjarmasin trying to obtain a personal identity in this case is a resident ID card (KTP), as a legitimate citizen in the Indonesian law. Therefore, if the identity card (KTP) is damaged/lost/change in identities or resident data, the community of Banjarmasin is trying to get the service from the population Office and civil registration of Banjarmasin City to the community. The goal that is to be achieved in this research is to find out what are the changes in the renewal service in the city of population and civil registration services of Banjarmasin and the renewal factors of resident identity card (KTP) and civil registration service of Banjarmasin City. This research is done with a qualitative approach with the type of research used is deskritif, the location of research in the Department of Population and Civil registration of Banjaramasin City. The technique of collecting data on this research is observation and interviews. The results of the renewal service of E-KTP of population and civil registration services Banjarmasin City can be concluded that in general, the renewal of E-KTP service in the population Office and civil registration of Banjarmasin City is not maximally well. The inhibitory factor in the renewal of E-KTP service in the population Office and civil registration of the city of Banjarmasin is from service. Suggestion submitted to the Ministry of Occupation and civil registration of the city of Banjarmasin based on the conclusion, researchers suggested to the population service and civil registration of Banjarmasin City to fix indicators that are considered still less successful so that the renewal service E-KTP in the population office and civil registration of the city of Banjarmasin can be categorized well.
Pelatihan Pemanfaatan Limbah Jerami Sebagai Media untuk Budidaya Jamur Tiram Putih
Authors: Susilo  , Meitiyani  , Setyaningsih Maryanti
Published: June 2021

Oyster mushroom cultivation (Pleurotus sp.) is relatively easy, because it has a fairly good adaptability to the environment and is very suitable to be developed in tropical regions such as Indonesia. One of the factors that play a major role is the planting medium which is generally in the form of wood powder. The supply of sawdust is decreasing, so that alternative media is needed which contains lots of organic matter and cellulose. Straw is an alternative substitute for wood powder media because it is abundant. The purpose of this activity was to provide skills in mushroom cultivation by utilizing straw waste to PKK women in Cigaragol village, Ciulengsi, Bogor. The skills about cultivating oyster mushrooms need to be disseminated to the wider community to overcome the shortage of supply of oyster mushrooms on the market. The method used in this activity is by lecturing, discussing and practicing. The results of training activities on the cultivation of white oyster mushrooms have increased community knowledge and skills by 71.9% which includes the introduction of 31.6% white oyster mushroom service, the benefits of white oyster mushrooms 52.6%, 100% white oyster mushroom habitat, 57.9% white wood powder media for cultivation of oyster mushrooms, straw media as an alternative to 100% white oyster mushroom cultivation and 89.5% white oyster mushroom cultivation technique. Training activities greatly help improve the knowledge and skills of cultivating white oyster mushrooms and potentially increase community income.
Mengembalikan Kejayaan Industri Kerajinan Cadas Silakarang di Kabupaten Gianyar melalui Penerapan Teknologi Cadas Termokromik Buatan
Authors: Karyasa I Wayan , Astawa I Gede Putu , Pradnyana I Made Ardwi
Published: June 2021

Covid-19 pandemi affects most of craft industries, including the formerly fabulous Silakarang stone’s arts and crafts located in the Gianyar Regency, Bali Province. Environmental issues regarding to river basin ecological protection along Ubud and Sukawati Districts, the mining of basaltic stones from the river banks was restricted strictly by the government. Thus, the natural stones resources for the Silakarang crafts became more difficult to afford. Hence, todays the Silakarang stone craftsmen are more and more dependent on the so-called white stone of Yogya from Java Island. Artificial stone, composing of volcanic ashes, rice husk ash silica-carbon nanocomposite, Portland cement could be the solution the problem. The addition of thermochromic natural inorganic pigment to the mixture could improve their color and performance of the artificial stones. The implementation of the thermochromic artificial stones technology to solve the stone crafts problem was conducted through community service using the participatory action and learning system approuch. As results, the artificial thermochromic stone crafts and arts products could be well accepted by costumers and it impacted to better selling of the stone crafts. The craftsmen gained better income and the Silakarang stone arts and crafts enterprises could better survive among the pandemic time.
Pelatihan Budidaya Tanaman secara Hidroponik untuk Pemenuhan Kebutuhan Sayur Skala Rumah Tangga
Authors: Hayati Nur , Fitriyah Lina Arifah , Wijayadi Andri Wahyu
Published: June 2021

Desa Ngampel, Ngusikan, Jombang memiliki kondisi lingkungan ekstrim dengan kondisi tanah yang kering dan suhu yang panas sehingga lahan pertanian kurang cocok untuk ditanami sayuran. Kondisi demikian menyebabkan masyarakat hanya bergantung pada penjual sayur keliling dalam memenuhi kebutuhannya. Kegiatan Pengabdian Kepada Masyarakat (PKM) ini bertujuan untuk memberikan pengetahuan dan keterampilan kepada masyarakat dalam budidaya sayuran secara hidroponik sehingga dapat diaplikasikan untuk pemenuhan kebutuhan sayur skala rumah tangga. Sasaran kegiatan ini adalah ibu-ibu PKK berjumlah 25 orang. Tahapan kegiatan PKM meliputi empat tahap yaitu: tahap persiapan, tahap pelatihan, tahap pendampingan dan tahap evaluasi. Observasi lokasi dilaksanakan pada bulan Juli-Agustus 2019, kegiatan PKM yang berupa pelatihan dilaksanakan pada tanggal 12-13 Desember 2019 dan pendampingan dilaksanakan sampai tanggal 2 Pebruari 2020. Dari kegiatan yang dilakukan menunjukkan bahwa indikator keberhasilan kegiatan PKM telah tercapai. Indikator partisipasi peserta PKM berhasil dicapai dengan prosentase 100%. Indikator tingkat pemahaman peserta tercapai dengan ditunjukkan 92% peserta PKM telah memahami materi budidaya sistem hidroponik. Indikator tingkat keterampilan peserta tercapai, dengan 84% peserta PKM mampu mempraktikkan budidaya sayuran dengan sistem hidroponik.
Medial temporal lobe high-frequency activity at feedback signals error and predicts subsequent memory during spatial learning
Authors: Stevenson Rebecca , Janecek John , Larson Myra , Mnatsakanyan Lilit , Vadera Sumeet , Lin Jack , Yassa Michael
Published: June 2021

The ability to incorporate information about feedback is critical for associative learning. The medial temporal lobe (MTL) and prefrontal cortex (PFC) are thought to be involved in processing feedback as new associations are learned. However, the relative contributions of these regions to feedback processing and subsequent memory performance in humans are poorly understood. To address this question, we tested pre-surgical epilepsy patients with depth electrodes implanted in the MTL and PFC using a spatial memory task in which subjects learned object-location associations over time. We found increased high-frequency activity (HFA; 40-100 Hz), thought to reflect local excitatory activity, in the MTL and dorsolateral PFC (dlPFC) at feedback for high error trials. In the MTL, this HFA error signal predicted greater trial-by-trial decreases in error from one training block to the next indicating that these signals are involved in updating memory representations or modifying incorrect associations during learning. The opposite pattern of activity was observed during retrieval, with greater MTL and dlPFC HFA predicting lower error, replicating previous results from our group. Overall, these data suggest putative mechanisms for the learning of object-location associations.
Association between ZFHX3 and PRR X1 polymorphisms and atrial fibrillation susceptibility
Authors: Wu Liting , Chu Ming , Zhuang Wenfang
Published: June 2021

Background Atrial fibrillation (AF) is a common, sustained cardiac arrhythmia. Recent studies have reported an association between ZFHX3/PRRX1 polymorphisms and AF. In this study, a meta-analysis was conducted to confirm these associations. Methods The PubMed, Embase, and Wanfang databases were searched, covering all publications before July 20, 2020. Results Overall, seven articles including 3,674 cases and 8,990 healthy controls for ZFHX3 rs2106261 and 1045 cases and 1407 controls for PRRX1 rs3903239 were included. The odds ratio (OR) [95% confidence interval (CI)] was used to assess the associations. Publication bias was calculated using Egger’s and Begg’s tests. We found that the ZFHX3 rs2106261 polymorphism increased AF risk in Asians (for example, allelic contrast: OR [95% CI]: 1.39 [1.31–1.47], P < 0.001). Similarly, strong associations were detected through stratified analysis using source of control and genotype methods (for example, allelic contrast: OR [95% CI]: 1.51 [1.38–1.64], P < 0.001 for HB; OR [95% CI]: 1.31 [1.21–1.41], P < 0.001 for PB; OR [95% CI]: 1.55 [1.33–1.80], P < 0.001 for TaqMan; OR [95% CI]: 1.31 [1.21–1.41], P < 0.001 for high-resolution melt). In contrast, an inverse relationship was observed between the PRRX1 rs3903239 polymorphism and AF risk (C-allele vs. T-allele: OR [95% CI]: 0.83 [0.77–0.99], P = 0.036; CT vs. TT: OR [95% CI]: 0.79 [0.67–0.94], P = 0.006). No obvious evidence of publication bias was observed. Conclusion In summary, our study suggests that the ZFHX3 rs2106261 and PRRX1 rs3903239 polymorphisms are associated with AF risk, and larger case-controls must be carried out to confirm the above conclusions.
Impact of interface trap charges on Junctionless double and triple metal gate High-k Gate All Around Nanowire FET based Alzheimer Biosensor
Authors: Chaujar Rishu , Yirak Mekonnen Getnet
Published: June 2021

In this work, junctionless double and triple metal gate high-k gate all around nanowire field-effect transistor-based APTES biosensor has been developed to study the impact of ITCs on device sensitivity. The analytical results were authenticated using ‘‘ATLAS-3D’’ device simulation tool. Effect of different interface trap charge on the output characteristics of double and triple metal gate high-k gate all around junctionless NWFET biosensor was studied. Output characteristics, like transconductance, output conductance,drain current, threshold voltage, subthreshold voltage and switching ratio, including APTES biomolecule, have been studied in both devices. 184% improvement has been investigated in shifting threshold voltage in a triple metal gate compared to a double metal gate when APTES biomolecule immobilizes on the nanogap cavity region under negative ITCs. Based on this finding, drain off-current ratio and shifting threshold voltage were considered as sensing metrics when APTES biomolecule immobilizes in the nanogap cavity under negative ITCs which is significant for Alzheimer's disease detection. We signifies a negative ITC has a positive impact on our proposed biosensor device compared to positive and neutral ITCs.
Potential of Aspergilus Niger MT809753 for Bio-treatment Paper Industry Wastewater After Screening Effective Factors on Production of Cellulase Enzyme by Plackett-burman Design
Authors: El-Sesy Marwa , Aly Amira M
Published: June 2021

A microorganism capable of degrading cellulose present in rice straw was isolated from wastewater samples and identified as Aspergilus niger MT809753 by 18S rDNA. In the present study various cheap agronomic cellulosic wastes as (cotton seed husks, barley straw, rice straw and maize straw) were utilized as crude inducers for the cellulase enzyme production and represent the carbon source for isolates where cellulose activity was measured by (DNS) method. The highest cellulases enzyme production was obtained by fungal isolate Aspergilus niger MT809753 within 24 hours (0.532 IU/ml) using rice straw. Plackett-Burman design was used as conventional method for statistically screening of different variables. Nine variables of the production process were selected. The results illustrate those seven variables, namely as (inoculum size, substrate concentration, incubation temperature, pH, shaking conditions, and incubation time and peptone concentration) had influence with high confidence levels, while the remaining two variables did not show a significant effect on cellulase production. After using response optimization the experiment was performed and the obtained cellulase production was 1.08 IU/ml. A bench scale study was performed to examine paper industry wastewater treatment efficiency by Aspergillus Niger MT809753. Results reveal that organisms have proved their bioremediation potency in treatment of paper industry effluent. The importance of the research stems from the fact that it sheds light on the role of some fungi in the production of the cellulase enzyme. So our goal is to obtain local isolates from fungi having a high ability to produce the cellulase enzyme, as well as developing an effective treatment processes to get rid of environmental cellulosic pollution and utilization of cellulosic wastes as cheap carbon sources.
Evaluation of Stormwater Detention Pond to Prepare Recreational Water Quality
Authors: Liu Pao-Wen Grace , Sun Sowa , Lin You Ping , Wang Zhi-Xian , Lin Guan Xin
Published: June 2021

Jenteh Detention Pond (JDP) composing of north and south ponds was designed for lessen extreme floods and disaster relief. This water monitoring project was motivated by concerning hygiene contamination once the JDP is opened as a recreational water park. The Surface Water Classification and Water Quality Standards (Taiwan Environmental Protection Agency) and Bacterial Water Quality Standards (United State EPA) were evaluated and then applied to examine the yearly samples: total coliform (TC), dissolved oxygen (DO), ammonia-nitrogen (NH3-N), and total phosphorus (TP). Significant correlation (0.442-0.946) between precipitation and the TC was concluded so that the South Pond should be alerted for the potential fecal contamination, once encountered torrential rains or extreme floods. Elevated DO over 130% of the corresponding saturation implied deteriorated water quality in the South Pond. High TP (0.36± 0.19 - 1.89± 0.52 mg/L) in both ponds revealed possibility of eutrophication. However, because JDP was not designed as a full-body-use recreational park, the fecal indicator in the bacterial water quality standards did not prevent the two ponds from leisure activity except flooding days. With overall statistical analysis, the water quality found be generally adequate by attaining the reference base line of Category C in the surface water classification.
Characteristics of the Paediatric Foot Arches According to Body Mass Among Primary School Students in Wrocław, Poland
Authors: Górna Sara , Pazdro-Zastawny Katarzyna , Basiak-Rasała Alicja , Kolator Mateusz , Krajewska Joanna , Zatoński Tomasz
Published: June 2021

Introduction:The purpose of the study conducted within the framework of the, “Let’s Get the Kids Moving” project was to determine the association between longitudinal and transverse children’s foot arch and their body mass, gender, and age.Materials and method:T he pro-health initiative “Let’s Get the Kids Moving” was created by researchers of the Medical University and the Run for Health Foundation. A total of 655 children (51.5% boys) aged 7 - 10 years from primary schools in southwestern Poland participated in the study. The mean age of the examined pupils was 8.7± 0 .8 years. In all children, we assessed anthropometric measurements (body mass and height) and longitudinal and transverse arch of the foot under a load of their weight. A two-dimensional (2D) foot scanner (Sensor Medica, Italy) was used to examine the plantar part of the pupils’ foot.Results:The obtained data from 655 pupils revealed that excessive body mass predisposed to lower longitudinal and transverse arching. The foot shape was not differentiated by children's gender and age in the youngest age group.Conclusions:Screening of school-aged children's footprints allows for early detection of abnormalities in the shape of the pupil’s feet. This enables the implementation of further early diagnosis for the detection of functional or structural flatfoot in children.
Analysis of Neurological Clinical Manifestations and Diagnostic Workups in Pediatric COVID-19: A Systematic Review
Authors: Octavius Gilbert Sterling , Chandra Shally , Wijaya Jeremiah Hilkiah , Juliansen Andry
Published: June 2021

Background While numerous studies report neurological manifestations of COVID-19, there are fewer reviews in the pediatric population, focusing solely on the manifestations.Aim This systematic review aims to describe the clinical manifestations and laboratory findings of pediatric patients with neurological manifestations of COVID-19.Methods We searched Pubmed, Science Direct, Medline, Scielo, Medrxiv, Research Square, SSRN, and Biorxiv with the appropriate keywords. Inclusion criteria include new-onset neurological manifestations with a time correlation to the confirmed COVID-19 with a further diagnostic work-up. We use The Joanna Briggs Institute's (JBI) essential evaluation checklist and Newcastle Ottawa Quality Assessment Scale (NOS) to evaluate the quality of the studies. The protocol for this systematic review has been registered in The international Prospective Register of Systematic Review (PROSPERO) database (CRD42021228671).Results 31 articles were ultimately chosen with 42 patients (26 male) included in this systematic review. The neurological diagnosis was mostly of the CNS classification (38/42) with a predomination of brain disorders (22/38). Most patients had lymphopenia, elevated C-reactive protein, procalcitonin, ferritin, creatinine kinase, blood urea nitrogen, D-dimer, and erythrocyte sedimentation rate. In the CNS group, CSF monocytes (55%; 11–80%), CSF polymorphonuclear (PMN) cells (15%; 0–96%), CSF red blood cells (282 cells/mm3; 2-4000 cells/mm3), and opening pressure (34.5 cm/H2O; 25–100 cm/H2O) were elevated.Conclusions Our systematic review found that neurological manifestations in COVID-19 occurred more often in males, with a majority being CNS disorders. A majority had a favorable outcome. More studies are needed in order to elucidate the viral mechanisms of SARS-CoV-2 in CSF.
Normalization and Outlier Removal in Class Center-Based Firefly Algorithm for Missing Value Imputation
Authors: Nugroho Heru , Utama Nugraha Priya , Surendro Kridanto
Published: June 2021

Missing data is one of the factors often causing incomplete data in research. Data normalization and missing value handling were considered major problems in the data pre-processing stage, while classification algorithms were adopted to handle numerical features. Furthermore, in cases where the observed data contains outliers, the missing values’ estimated results are sometimes unreliable, or even differ greatly from the true values. This study aims to proposed combination of normalization and outlier removal’s before imputing missing values using several methods, mean, random value, regression, multiple imputation, KNN, and C3-FA. Experimental results on the sonar dataset show normalization and outlier removal’s effect in these imputation methods. In the proposed C3-FA method, this produced accuracy, F1-Score, Precision, and Recall values of 0.906, 0.906, 0.908, and 0.906, respectively. Based on the KNN classifier evaluation results, this value outperformed the other five (5) methods. Meanwhile, the results for RMSE, Dks, and r obtained from combining normalization and outlier removal’s in the C3-FA method were 0.02, 0.04, and 0.935, respectively. This shows that the proposed method is able to reproduce the real values ​​of the data or the prediction accuracy and maintain the distribution of the values ​​or the distribution accuracy.
Upregulated lncARAT in Schwann Cells Promotes Axonal Regeneration through Recruiting Macrophages and Inducing Macrophages M2 Polarization
Authors: Yin Gang , Lin Yaofa , Wang Peilin , Zhou Jun , Lin Haodong
Published: June 2021

Background: Axonal regeneration following peripheral nerve injury largely depends on a favorable microenvironment. Schwann cells (SCs) play a crucial role in axonal regeneration by interacting with macrophages, but the mechanisms underlying macrophages recruitment and polarization remain unclear. Methods: The total RNA of crushed sciatic nerves and intact contralateral nerves was extracted and used to RNA-sequencing (RNA-seq). The differentially expressed long noncoding RNA (lncRNA) and mRNAs were analyzed using bioinformatics analysis, and were verified using qPCR and western blot analysis. The putative role of lncRNA in nerve regeneration was analyzed in vitro and in vivo. Macrophage polarization phenotype was identified by assessing IL-10, Arg-1, and CD206.Results: Here we identified an lncRNA, termed Axon Regeneration-Associated Transcript (lncARAT), upregulated in SCs and SCs-derived exosomes after crushed sciatic nerves (CSN). LncARAT contributed to axonal regeneration and improved motor functional recovery. Mechanistically, lncARAT epigenetically activated CCL2 expression by recruiting KMT2A to CCL2 promoter, which resulted in an increased H3K4 trimethylation and CCL2 transcription in SCs. CCL2 upregulation facilitated the infiltration of macrophages into the injured nerves. Meanwhile, lncARAT-enriched exosomes were released from SCs and incorporated into macrophages. Once in macrophage, lncARAT functioned as an endogenous sponge to adsorb miRNA-329-5p, resulting in an increased SOCS2 expression, which facilitated macrophage M2 polarization through a STAT1/6-dependent pathway, thus promoted axonal regeneration. Conclusions: LncARAT may serve as a promising therapeutic avenue for peripheral nerve repair.
Effects of Compacted Forms (Stick and Pellet Fuels) of Rice Husks as Fuel Use on Silica Solubility for Fertilizer Use
Authors: Sekifuji Ryoko , Chieu Le Van , Tateda Masafumi , Takimoto Hiroshi
Published: June 2021

Extremely lightweight of rice husks due to low specific gravity is disadvantageous for accelerating its usage. To overcome this disadvantage, rice husks are sometimes compacted, that is, pelletized or solidified to assist easy and cheap collection and hauling. However, the compaction process may negatively affect the properties of silica in the husk. In this study, the influence of the compaction process was studied through the solubility and physical properties of silica. Two types of fuels, pellet and stick fuels, were synthesized and their influence on the properties of the fuel ash was evaluated. Consequently, stick fuels were better than pellet fuels in terms of fertilizer use of the ash during their use in residential stoves and small commercial heaters. Contrastingly, rice husks in the existing form were better than stick fuels under washing conditions; however, rice husks had disadvantages in terms of cost and handling during collection and hauling. Selection of rice husks in their existing form or compacted rice husks must be carefully considered based on the business approaches of the stakeholders.
SorCS3 Promotes the Internalization of p75NTR to Inhibit Glioma Progression
Authors: Zhang Yanqiu , Li Yue , Fan Yuhua , Zhao Baoshan , Liang Huan , Li Zhiliang , Xu Haiyan , Li Dongliang
Published: June 2021

Background: Glioma is a fatal malignancy caused by dysregulation of cellular signal transduction. Internalization plays a key role in maintaining signalling balance. SorCS3 is involved in nerve cell receptor internalization. However, the impact of SorCS3 on the biological processes involved in glioma has not yet been reported. Here, we highlight the potential of SorCS3-mediated regulation of signalling receptor internalization as a rational target for therapeutic intervention in glioma.Methods: SorCS3 expression was analysed in the TCGA and CGGA databases and in tissue microarrays. The effects of SorCS3 on the proliferation and metastasis of glioma cells were examined in vitro and in vivo with Transwell, wound healing, EdU incorporation and nude mouse tumorigenicity assays. Fluorescent 5-FAM, SE-labelled proteins were used to detect the internalization of SorCS3 in glioma cells. Immunofluorescence and Co-IP assays were conducted to investigate the downstream effector of SorCS3. Moreover, Dynasore and Ro 08-2750, inhibitors of internalization and NGF binding to p75NTR, respectively, were used to validate the biological functions of SorCS3 in glioma.Results: Our data demonstrated that SorCS3 was downregulated in glioma tissues and closely related to favourable prognosis. Overexpression of SorCS3 inhibited the proliferation and metastasis of glioma cells in vitro and in vivo, while silencing of SorCS3 exerted the opposite effects. Mechanistic investigations showed that SorCS3 bound to p75NTR, which subsequently increased the internalization of p75NTR, and then transported p75NTR to the lysosome for degradation, ultimately contributing to inhibition of glioma progression.Conclusions: Our work suggests that SorSC3 is a marker of promising prognosis in glioma patients and suggests that SorCS3 regulates internalization, which plays an important role in inhibiting glioma progression.
Comparison of OPD-Scan III and Pentacam in Measuring Biological Measurements in Cataract Patients with Corneal Astigmatism
Authors: Li Panpan , Song Yu , Tu Yuanyuan , Yuan You
Published: June 2021

Purpose To assess the accuracy of OPD-Scan III in measuring keratometry, astigmatism, axes and intraocular lens (IOL) power in cataract patients with corneal astigmatism.Methods Cataract patients with keratometric astigmatism (KA) measurements greater than 0.75 D were included. Measurements were taken with OPD-Scan III and Pentacam devices. The following measurements were obtained via these 2 devices: keratometry in the steepest axis (Ks) and ﬂattest axis (Kf), KA, axis, Jackson crossed cylinder of powers J0 and J45 and IOL power.Results Seventy eyes of 63 patients were included to analyze the repeatability of the two devices. The ICCs of each index were greater than 0.85 via the OPD-Scan III. Seven hundred thirty-two eyes of 516 patients were included to analyze the differences, correlations and agreements of the two devices. Statistically significant differences between the devices were found in Ks, Kf, axis, J0 and J45 (all p < 0.01). There were significant correlations found between the two devices in each index (all p < 0.01). The 95% limits of agreement (LOAs) for Ks, Kf, KA, axis, J0, J45 and IOL power were (-0.34, 0.44) D, (-0.24, 0.38) D, (-0.46, 0.48) D, (-10.20, 12.92) °, (-0.24, 0.24) D, (-0.27, 0.35) D and (-0.32, 0.22) D, respectively.Conclusion OPD-Scan III had high accuracies for measuring keratometry and IOL power, but the accuracies for measuring astigmatism and the axis were poor in eyes with moderate to high astigmatism.
Mechanosensitive Cation Channel Piezo1 Contributes To Ventilator-Induced Lung Injury By Activating RhoA/ROCK1 In Rats
Authors: Zhang Yang , Jiang Lulu , Huang Tianfeng , Lu Dahao , Song Yue , Wang Lihui , Gao Ju
Published: June 2021

Background: Mechanical ventilation can induce or aggravate lung injury, which is termed ventilator‑induced lung injury. Piezo1 is a key element of the mechanotransduction process and can transduce mechanical signals into biological signals by mediating Ca2+ influx, which in turn regulates cytoskeletal remodeling and stress alterations. We hypothesized that it plays an important role in the occurrence of ventilator‑induced lung injury, and we investigated the underlying mechanisms. Methods: High tidal volume mechanical ventilation and high magnitude cyclic stretch were performed on Sprague Dawley rats, and A549 and human pulmonary microvascular endothelial cells, respectively, to establish ventilator‑induced lung injury models. Immunohistochemical staining, flow cytometry, histological examination, enzyme-linked immunosorbent assay, western blotting, quantitative real-time reverse transcription-PCR and survival curves were used to assess the effect of Piezo1 on induction of lung injury, as well as the signaling pathways involved.Results: We observed that Piezo1 expression increased in the lungs after high tidal volume mechanical ventilation and in cyclic stretch-treated cells. Mechanistically, we observed the enhanced expression of RhoA/ROCK1 in both cyclic stretch and Yoda1-treated cells, while the deficiency or inhibition of Piezo1 dramatically antagonized RhoA/ROCK1 expression. Furthermore, blockade of RhoA/ROCK1 signaling using an inhibitor did not affect Piezo1 expression. GSMTx4 was used to inhibit Piezo1, which alleviated ventilator‑induced lung injury-induced pathologic changes, water content and protein leakage in the lungs, and the induction of systemic inﬂammatory mediators, and improved the 7-day mortality rate in the model rats. Conclusions: These findings indicate that Piezo1 affects the development and progression of ventilator‑induced lung injury through promotion of RhoA/ROCK1 signaling.