bluuebunny/crossref_metadata_2025 huggingface dataset:
Dihydropyridine Enhances the Antioxidant Capacities of Lactating Dairy Cows under Heat Stress Condition
Authors: Yu Meng-Fei , Zhao Xin-Mao , Cai Hang , Yi Jian-Ming , Hua Guo-Hua
Published: October 2020

Heat stress (HS), a nonspecific response to environmental heat, can seriously affect dairy cow health. Feed additives may alleviate HS in dairy cows by improving rumen fermentation efficacy, stimulating feed consumption, enhancing vasodilation, and/or improving antioxidant capacity. The temperature–humidity index (THI) indicates that spring is a non-HS season, and summer is an HS season. HS results in the decrease in dairy cow antioxidant capacities. Our results indicated the decrease in superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and total antioxidation (T-AOC) levels and the increase in malondialdehyde (MDA) level during HS season. Meanwhile, antioxidant indexes (SOD, GSH-Px, and T-AOC) were positively correlated with milk yield (p < 0.01), whereas MDA exhibited a significant negative correlation with milk yield (p < 0.05). In addition, the effects of dihydropyridine (DHP) on antioxidant capacity and ruminal microbial communities in dairy cows under HS were investigated. During summer, dairy cows were randomly assigned into two groups under HS, including a standard diet (S-ND) group and standard diet with 3 g/day/cow DHP (S-D) group. DHP treatment significantly restored SOD and GSH-Px levels under HS. Denaturing gradient gel electrophoresis results indicated that the DHP altered ruminal bacterial community mainly composed Proteobacteria and Firmicutes in dairy cows under HS. Our results suggest that DHP can enhance the antioxidant abilities of dairy cows with favorable effects on ruminal microbial communities under HS, further alleviating HS on dairy cows.
Structural Antibiotic Surveillance and Stewardship via Indication-Linked Quality Indicators: Pilot in Dutch Primary Care
Authors: van der Velden Alike W. , van Triest Mieke I. , Schoffelen Annelot F. , Verheij Theo J. M.
Published: October 2020

Insight into antibiotic prescribing quality is key to general practitioners (GPs) to improve their prescribing behavior and to national antibiotic surveillance and stewardship programs. Additionally to numbers of prescribed antibiotics, quality indicators (QIs) linked to the clinical indication for prescribing are urgently needed. The aim of this proof of concept study was to define indication-linked QIs which can be easily implemented in Dutch primary care by collaborating with data-extraction/processing companies that routinely process patient data for GP practices. An expert group of academic and practicing GPs defined indication-linked QIs for which outcomes can be derived from routine care data. QI outcomes were calculated and fed back to GPs from 44 practices, associations between QI outcomes were determined, and GPs’ opinions and suggestions with respect to the new set were captured using an online questionnaire. The new set comprises: (1) total number of prescribed antibiotics per 1000 registered patients and percentages of generally non-1st choice antibiotics; (2) prescribing percentages for episodes of upper and lower respiratory tract infection; (3) 1st choice prescribing for episodes of tonsillitis, pneumonia and cystitis in women. Large inter-practice variation in QI outcomes was found. The validity of the QI outcomes was confirmed by associations that were expected. The new set was highly appreciated by GPs and additional QIs were suggested. We conclude that it proved feasible to provide GPs with informative, indication-linked feedback of their antibiotic prescribing quality by collaborating with established data extraction/processing companies. Based on GPs’ suggestions the set will be refined and extended and used in the near future as yearly feedback with benchmarking for GPs and for national surveillance and stewardship purposes.
Effect of Ubiquinol on Glaucomatous Neurodegeneration and Oxidative Stress: Studies for Retinal Ganglion Cell Survival and/or Visual Function
Authors: Edwards Genea , Lee Yonghoon , Kim Martha , Bhanvadia Soham , Kim Keun-Young , Ju Won-Kyu
Published: October 2020

Oxidative stress is one of major causal factors in glaucomatous neurodegeneration. Ubiquinol promotes retinal ganglion cell (RGC) survival against glaucomatous insults such as oxidative stress. Here we investigated the effect of ubiquinol on RGC survival and/or visual function in mouse models of glaucoma and oxidative stress. DBA/2J and age-matched DBA/2J-Gpnmb+ (D2-Gpnmb+), which do not develop intraocular pressure elevation, or C57BL/6J mice were fed with ubiquinol (1%) or control diet daily for 5 or 2 months. We assessed RGC survival by Brn3a immunohistochemistry and measured expression levels of active and total BAX, peroxisome proliferator-activated receptor-gamma coactivator 1α, transcription factor A (TFAM) and oxidative phosphorylation (OXPHOS) complex protein. Following induction of oxidative stress by paraquat injection, we also assessed visual function. In glaucomatous retina, ubiquinol supplementation significantly promoted RGC survival, blocked BAX activation and increased TFAM and OXPHOS complex II protein expression. Also, ubiquinol supplementation ameliorated oxidative stress-induced visual dysfunction. These findings indicate that ubiquinol promotes RGC survival by increasing TFAM expression and OXPHOS complex II activity in glaucomatous neurodegeneration, and that ubiquinol enhances RGC survival and preserves visual function against oxidative stress. We propose that ubiquinol has a therapeutic potential for treating oxidative stress-associated glaucomatous neurodegeneration.
Dietary and Lifestyle Factors Modulate the Activity of the Endogenous Antioxidant System in Patients with Age-Related Macular Degeneration: Correlations with Disease Severity
Authors: Ulańczyk Zofia , Grabowicz Aleksandra , Cecerska-Heryć Elżbieta , Śleboda-Taront Daria , Krytkowska Elżbieta , Mozolewska-Piotrowska Katarzyna , Safranow Krzysztof , Kawa Miłosz Piotr , Dołęgowska Barbara , Machalińska Anna
Published: October 2020

Age-related macular degeneration (AMD) is a common cause of blindness in the elderly population, but the pathogenesis of this disease remains largely unknown. Since oxidative stress is suggested to play a major role in AMD, we aimed to assess the activity levels of components of the antioxidant system in patients with AMD. We also investigated whether lifestyle and dietary factors modulate the activity of these endogenous antioxidants and clinical parameters of disease severity. We recruited 330 patients with AMD (39 with early, 100 with intermediate and 191 with late form of AMD) and 121 controls in this study. At enrolment, patients’ dietary habits and physical activity were assessed, and each study participant underwent a thorough ophthalmologic examination. The activity of several components of the antioxidant system were measured in red blood cells and platelets using both kinetic and spectrophotometric methods. Patients with AMD consumed much lower levels of fatty fish and eggs than the control group (p = 0.008 and p = 0.04, respectively). In the nAMD group, visual acuity (VA) correlated positively with green vegetable consumption (Rs = +0.24, p = 0.004) and omega-3-rich oil intake (Rs = +0.17, p = 0.03). In the AMD group, the total physical activity MET score correlated positively with VA (Rs = +0.17, p = 0.003) and correlated negatively with the severity of AMD (Rs = −0.14, p = 0.01). A multivariate analysis of patients and controls adjusted for age, sex, and smoking status (pack-years) revealed that AMD was an independent variable associated with a lower RBC catalase (β = −0.37, p < 0.001) and higher PLT catalase (β = +0.25, p < 0.001), RBC GPx (β = +0.26, p < 0.001), PLT GPx (β = +0.16, p = 0.001), RBC R-GSSG (β = +0.13, p = 0.009), PLT R-GSSG (β = +0.12, p = 0.02) and RBC GSH transferase (β = +0.23, p < 0.001) activity. The activities of components of the antioxidant system were associated with disease severity and depended on dietary habits. The observed substantial increase in the activity of many critical endogenous antioxidants in patients with AMD further indicates that the required equilibrium in the antioxidant system is disturbed throughout the course of the disease. Our findings explicitly show that a diet rich in green vegetables, fish and omega-3-rich oils, supplemented by physical exercise, is beneficial for patients with AMD, as it might delay disease progression and help retain better visual function.
Performance of Fine-Tuning Convolutional Neural Networks for HEp-2 Image Classification
Authors: Taormina Vincenzo , Cascio Donato , Abbene Leonardo , Raso Giuseppe
Published: October 2020

The search for anti-nucleus antibodies (ANA) represents a fundamental step in the diagnosis of autoimmune diseases. The test considered the gold standard for ANA research is indirect immunofluorescence (IIF). The best substrate for ANA detection is provided by Human Epithelial type 2 (HEp-2) cells. The first phase of HEp-2 type image analysis involves the classification of fluorescence intensity in the positive/negative classes. However, the analysis of IIF images is difficult to perform and particularly dependent on the experience of the immunologist. For this reason, the interest of the scientific community in finding relevant technological solutions to the problem has been high. Deep learning, and in particular the Convolutional Neural Networks (CNNs), have demonstrated their effectiveness in the classification of biomedical images. In this work the efficacy of the CNN fine-tuning method applied to the problem of classification of fluorescence intensity in HEp-2 images was investigated. For this purpose, four of the best known pre-trained networks were analyzed (AlexNet, SqueezeNet, ResNet18, GoogLeNet). The classifying power of CNN was investigated with different training modalities; three levels of freezing weights and scratch. Performance analysis was conducted, in terms of area under the ROC (Receiver Operating Characteristic) curve (AUC) and accuracy, using a public database. The best result achieved an AUC equal to 98.6% and an accuracy of 93.9%, demonstrating an excellent ability to discriminate between the positive/negative fluorescence classes. For an effective performance comparison, the fine-tuning mode was compared to those in which CNNs are used as feature extractors, and the best configuration found was compared with other state-of-the-art works.
Simplified Estimation Method for Maximum Deflection in Bending-Failure-Type Reinforced Concrete Beams Subjected to Collision Action and Its Application Range
Authors: Kurihashi Yusuke , Masuya Hiroshi
Published: October 2020

As natural disasters have become increasingly severe, many structures designed to prevent rockfalls and landslides have been constructed in various areas. The impact resistance capacity of a reinforced concrete (RC) rock shed can be evaluated using its roof deflection. This study establishes a method for estimating the maximum deflection of a bending-failure-type RC beam, subjected to collisions that is based on the energy conservation concept—in which, the transmitted energy from a collision is equivalent to the energy absorbed by the beam. However, the following assumptions have never been confirmed: (1) The energy transmitted to the RC beam, due to the dropped weight, can be estimated by assuming a perfect plastic collision; and (2) the energy absorbed by the RC beam can be estimated by assuming plane conservation. In this study, these assumptions were verified using 134 previous test results of RC beams subject to weight collisions. In addition, we proposed a simple method for calculating the maximum deflection and its application scope. With this method, a performance-based impact-resistant design procedure for various RC structures can be established in the future. Moreover, this method will significantly improve the maintenance and management of existing RC structures subject to collisions.
Assessment of Optimum CaO Content Range for High Volume FA Based Concrete Considering Durability Properties
Authors: Lee Jaehyun , Lee Taegyu , Choi Hyeonggil , Lee Dong-Eun
Published: October 2020

There have been many studies on the effect of durability and compressive strength on the increase of the mixing rate of admixtures. However, there is no research that can provide a guide on the optimal mixture proportions for maintaining compressive strength and secure durability properties when using local materials. Therefore, the purpose of this article is to assess the durability and engineering performances of concrete based on local fly ash (FA), as well as to derive the optimum CaO content scope for ensuring durability. The results of this study were compared with the results of the previous study of high-volume ground-granulated blast-furnace slag (GGBFS) concrete. To achieve this, tests were carried out by increasing the admixture mixing rate in 10% increments from 0% to 70%. The unit water was set at 175 kg/m3 and the amount of binder was set at 330 kg/m3. It was found that the overall compressive strength of the hardened concrete decreased when the admixture mixing rate increased. In addition, the compressive strength of specimens tended to improve as all the CaO contents of the admixture types increased. When the durability properties were examined, it was found that the relative dynamic elasticity modulus and carbonation depth decreased, and the chloride penetration depth increased as the CaO content increased for both GGBFS and FA. The weight loss rate, however, remained similar. Based on the results of this study, the optimal CaO content that achieved satisfactory engineering and durability properties was found to be between 39% and 48% for FA. The results of this study will be able to offer guidelines for the mixture rates of FA when mixing durable concrete for use in the field. Additionally, these results are expected to be utilized as a basis for determining instructions relating to chemical composition in order to develop binders with improved durability.
General Moving Object Localization from a Single Flying Camera
Authors: Yow Kin-Choong , Kim Insu
Published: October 2020

Object localization is an important task in the visual surveillance of scenes, and it has important applications in locating personnel and/or equipment in large open spaces such as a farm or a mine. Traditionally, object localization can be performed using the technique of stereo vision: using two fixed cameras for a moving object, or using a single moving camera for a stationary object. This research addresses the problem of determining the location of a moving object using only a single moving camera, and it does not make use of any prior information on the type of object nor the size of the object. Our technique makes use of a single camera mounted on a quadrotor drone, which flies in a specific pattern relative to the object in order to remove the depth ambiguity associated with their relative motion. In our previous work, we showed that with three images, we can recover the location of an object moving parallel to the direction of motion of the camera. In this research, we find that with four images, we can recover the location of an object moving linearly in an arbitrary direction. We evaluated our algorithm on over 70 image sequences of objects moving in various directions, and the results showed a much smaller depth error rate (less than 8.0% typically) than other state-of-the-art algorithms.
Parallel Broadband Femtosecond Reflection Spectroscopy at a Soft X-Ray Free-Electron Laser
Authors: Engel Robin Y. , Miedema Piter S. , Turenne Diego , Vaskivskyi Igor , Brenner Günter , Dziarzhytski Siarhei , Kuhlmann Marion , Schunck Jan O. , Döring Florian , Styervoyedov Andriy , Parkin Stuart S.P. , David Christian , Schüßler-Langeheine Christian , Dürr Hermann A. , Beye Martin
Published: October 2020

X-ray absorption spectroscopy (XAS) and the directly linked X-ray reflectivity near absorption edges yield a wealth of specific information on the electronic structure around the resonantly addressed element. Observing the dynamic response of complex materials to optical excitations in pump–probe experiments requires high sensitivity to small changes in the spectra which in turn necessitates the brilliance of free electron laser (FEL) pulses. However, due to the fluctuating spectral content of pulses generated by self-amplified spontaneous emission (SASE), FEL experiments often struggle to reach the full sensitivity and time-resolution that FELs can in principle enable. Here, we implement a setup which solves two common challenges in this type of spectroscopy using FELs: First, we achieve a high spectral resolution by using a spectrometer downstream of the sample instead of a monochromator upstream of the sample. Thus, the full FEL bandwidth contributes to the measurement at the same time, and the FEL pulse duration is not elongated by a monochromator. Second, the FEL beam is divided into identical copies by a transmission grating beam splitter so that two spectra from separate spots on the sample (or from the sample and known reference) can be recorded in-parallel with the same spectrometer, enabling a spectrally resolved intensity normalization of pulse fluctuations in pump–probe scenarios. We analyze the capabilities of this setup around the oxygen K- and nickel L-edges recorded with third harmonic radiation of the free electron laser in Hamburg (FLASH), demonstrating the capability for pump–probe measurements with sensitivity to reflectivity changes on the per mill level.
Optimal Mixture Designs for Heavy Metal Encapsulation in Municipal Solid Waste Incineration Fly Ash
Authors: Wang Ying , Ni Wen , Zhang Siqi , Li Jia , Suraneni Prannoy
Published: October 2020

Mixing municipal solid waste incineration fly ash (MSWIFA) with industrial by-products such as ground granulated blast furnace slag (GGBFS) and ladle furnace slag (LFS) can lead to a hardened system which can encapsulate the heavy metals present in the MSWIFA. The objective of this study is to find optimal mixture designs to effectively encapsulate these heavy metals. The nature of the hydrates and the strength of the mixtures are studied to develop a sustainable and practical construction material incorporating MSWIFA. Heavy metals including Cr, Cu, Zn and Cd are safely encapsulated in several developed mixtures with leachate concentration below EPA drinking water limit. The encapsulation behavior is complex and depends on metal type, age of testing, and hydration products. In general, mixtures containing LFS have more aluminate hydrates, and show greater encapsulation capacity for most heavy metals. However, they also generally show significant Sb leaching. Mixtures which show satisfactory encapsulation for all ions and adequate strength development are identified. Three ideal mixtures, including one containing zero cement, are identified which satisfy both leaching and strength requirements.
Experimental Characterization of Two-Phase Aerated Liquid Ethanol and Jet A Spray Flames
Authors: Smith Cary , Gragston Mark , Wu Yue , Zhang Zhili
Published: October 2020

This work provides an analysis of an air-assisted atomizing nebulizer’s spray characteristics and combustion behaviors via application of various diagnostics. Two-phase far-field flows and flames of liquid ethanol and practical aviation fuel Jet A were characterized for the droplet distributions, fuel concentrations, and flame dynamics by shadowgraph, Mie scattering, and chemiluminescence imaging of CH*, C2*, and CO2*. Both instantaneous and time-averaged measurements of the two-phase flow and flame characteristics were obtained. Shadowgraphs were used to measure the probability distribution of the droplet diameters. Mie scattering was used to map fuel concentration and obtain the liquid spray cone angles. Estimates of the lifted flame height were obtained by both shadowgraph and Mie scattering, which were verified by the chemiluminescence images. The effect of flow rates on parameters such as flow characteristic, spray cone angle, and drop size spectrum were quantitatively characterized for both ethanol and practical aviation fuel Jet A.
Wind-Driven Hydrodynamics in the Shallow, Micro-Tidal Estuary at the Fangar Bay (Ebro Delta, NW Mediterranean Sea)
Authors: F-Pedrera Balsells Marta , Grifoll Manel , Espino Manuel , Cerralbo Pablo , Sánchez-Arcilla Agustín
Published: October 2020

This article investigates water circulation in small-scale (~10 km2), shallow (less than 4 m) and micro-tidal estuaries. The research characterizes the hydrodynamic wind response in these domains using field data from Fangar Bay (Ebro Delta) jointly with three-dimensional numerical experiments in an idealized domain. During calm periods, field data in Fangar Bay show complex water circulation in the inner part of the estuary owing to its shallow depths and positive estuarine circulation in the mouth. Numerical experiments are conducted to investigate wind-induced water circulation due to laterally varying bathymetry. For intense up-bay wind conditions (wind intensities greater than 9 m·s−1), an axially symmetric transverse structure occurs with outflow in the central channel axis and inflow in the lateral shallow areas. These numerical results explain the water circulation observed in Fangar Bay during strong wind episodes, highlighting the role of the bathymetry in a small-scale environment. During these episodes, the water column tends to homogenize rapidly in Fangar Bay, breaking the stratification and disrupting estuarine circulation, consistent with other observations in similar domains.
Towards Semi-Automatic Generation of a Steady State Digital Twin of a Brownfield Process Plant
Authors: Sierla Seppo , Sorsamäki Lotta , Azangoo Mohammad , Villberg Antti , Hytönen Eemeli , Vyatkin Valeriy
Published: October 2020

Researchers have proposed various models for assessing design alternatives for process plant retrofits. Due to the considerable engineering effort involved, no such models exist for the great majority of brownfield process plants, which have been in operation for years or decades. This article proposes a semi-automatic methodology for generating a digital twin of a brownfield plant. The methodology consists of: (1) extracting information from piping and instrumentation diagrams, (2) converting the information to a graph format, (3) applying graph algorithms to preprocess the graph, (4) generating a simulation model from the graph, (5) performing manual expert editing of the generated model, (6) configuring the calculations done by simulation model elements and (7) parameterizing the simulation model according to recent process measurements in order to obtain a digital twin. Since previous work exists for steps (1–2), this article focuses on defining the methodology for (3–5) and demonstrating it on a laboratory process. A discussion is provided for (6–7). The result of the case study was that only few manual edits needed to be made to the automatically generated simulation model. The paper is concluded with an assessment of open issues and topics of further research for this 7-step methodology.
Brain Tumor-Derived Extracellular Vesicles as Carriers of Disease Markers: Molecular Chaperones and MicroRNAs
Authors: Vitale Alessandra Maria , Santonocito Radha , Vergilio Giuseppe , Marino Gammazza Antonella , Campanella Claudia , Conway de Macario Everly , Bucchieri Fabio , Macario Alberto J. L. , Caruso Bavisotto Celeste
Published: October 2020

Primary and metastatic brain tumors are usually serious conditions with poor prognosis, which reveal the urgent need of developing rapid diagnostic tools and efficacious treatments. To achieve these objectives, progress must be made in the understanding of brain tumor biology, for example, how they resist natural defenses and therapeutic intervention. One resistance mechanism involves extracellular vesicles that are released by tumors to meet target cells nearby or distant via circulation and reprogram them by introducing their cargo. This consists of different molecules among which are microRNAs (miRNAs) and molecular chaperones, the focus of this article. miRNAs modify target cells in the immune system to avoid antitumor reaction and chaperones are key survival molecules for the tumor cell. Extracellular vesicles cargo reflects the composition and metabolism of the original tumor cell; therefore, it is a source of markers, including the miRNAs and chaperones discussed in this article, with potential diagnostic and prognostic value. This and their relatively easy availability by minimally invasive procedures (e.g., drawing venous blood) illustrate the potential of extracellular vesicles as useful materials to manage brain tumor patients. Furthermore, understanding extracellular vesicles circulation and interaction with target cells will provide the basis for using this vesicle for delivering therapeutic compounds to selected tumor cells.
Vegetable-Milk-Based Yogurt-Like Structure: Rheological Properties Influenced by Gluten-Free Carob Seed Flour
Authors: Froiio Francesca , Cristiano Maria Chiara , Mancuso Antonia , Iannone Michelangelo , Paolino Donatella
Published: October 2020

There is a constant increase in the attention being paid to food quality and the effects of food on human health among consumers. Vegetable milk is among the foods whose consumption worldwide has increased because, when compared to animal-derived milk, it offers numerous benefits for human health. The aim of this research work was to use vegetable milk to obtain yogurt-like products enriched with different concentrations of carob seed flour, which has a double function: to modify, and thus perfect, the rheological characteristics of vegetable-milk-based yogurt-like samples and to increase their nutritional value. The rheological parameters of the obtained samples were studied both in static and dynamic conditions, confirming that carob seed flour, especially at the highest used concentrations (0.75%; 1%), allows one to obtain products characterized by a good stability and suitable rheological characteristics. The obtained yogurt-like products may also be consumed by celiac subjects, since carob seed flour is a gluten-free flour, and allow celiac consumers to combine a gluten-free diet with a diet free of animal derivatives. Furthermore, the addition of carob flour allows one to obtain a tasty product thanks to the sweet taste of the carob seed flour.
Xylitol Production from Exhausted Olive Pomace by Candida boidinii
Authors: López-Linares Juan Carlos , Ruiz Encarnación , Romero Inmaculada , Castro Eulogio , Manzanares Paloma
Published: October 2020

In this work, the production of xylitol from a hemicellulosic hydrolysate of exhausted olive pomace (EOP), a residue originated in the olive oil production process by Candida boidinii, was assessed. The hydrolysate was obtained by dilute acid pretreatment of EOP at 170 °C and 2% H2SO4 (w/v). A previous detoxification step of the hydrolysate was necessary, and its treatment with activated charcoal and ion-exchange resin was evaluated. Prior to fermentation of the hydrolysate, fermentation tests in synthetic media were performed to determine the maximum xylitol yield and productivity that could be obtained if inhibitory compounds were not present in the medium. In addition, the glucose existing in the media exerted a negative influence on xylitol production. A maximum xylitol yield of 0.52 g/g could be achieved in absence of inhibitor compounds. Fermentation of the hemicellulosic hydrolysate from EOP after detoxification with ion-exchange resin resulted in a xylitol yield of 0.43 g/g.
Effect of Fluoride on Germination, Early Growth and Antioxidant Enzymes Activity of Three Winter Wheat (Triticum aestivum L.) Cultivars
Authors: Pelc Justyna , Śnioszek Martyna , Wróbel Jacek , Telesiński Arkadiusz
Published: October 2020

This paper assesses the impact of sodium fluoride on the morphological parameters and activity of catalase and peroxidase during the germination and root growth phases of three winter wheat cultivars: Tobak, Dalewar, and Arkadia. During examination, the seeds were placed on plastic Petri dishes with an NaF solution at concentrations of 0 (control), 2.5, 5.0, 8.0, and 10.0 mmol dm−3. The obtained results have shown a decrease in germination, inhibition of root growth, and inhibition of catalase activity, both in the embryos and roots of all tested winter wheat cultivars. The observed effects have been strengthened with the increase of the fluoride concentration. However, the effect of NaF on the peroxidase activity has been dependent on the wheat cultivar. It is difficult to state unequivocally which of the tested winter wheat cultivars has been characterized by the highest sensitivity to fluoride. An η2 analysis has confirmed that the NaF concentration has a greater effect than the winter wheat cultivar on the activity of the determined antioxidant enzymes.
A Real Witcher—Slavic or Universal; from a Book, a Game or a TV Series? In the Circle of Multimedia Adaptations of a Fantasy Series of Novels “The Witcher” by A. Sapkowski
Authors: Gawroński Sławomir , Bajorek Kinga
Published: October 2020

A series of novels about a witcher, written by Andrzej Sapkowski almost thirty years ago, has now become an inspiration for the creation of mass productions of mainstream popular culture—film and multimedia adaptations for use in computer games. It is one of the few examples of global messages of mass culture being based on Polish creativity. The recognition of “The Witcher”, due to the Netflix production, soon contributed to building the national pride of Polish people, and at the same time sparked a discussion in Central and Eastern European countries on the consequences of the multimedia adaptation of Andrzej Sapkowski’s prose. Questions about the dissonance between the Slavic and universal dimensions of “The Witcher” in relation to the original novels and their adaptations are a part of the traditional discourse on the adaptability of literature and its consequences for the reception by the audience. This article tries to capture the specific character of the adaptations of Andrzej Sapkowski’s literature from the point of view of typology, known from the literature of the subject, as well as to answer the question about the consequences of the discrepancy between the original book and its adaptations in the form of a film, a TV series, and computer games. The considerations in the article were based on the literature analysis and the research based on the existing sources.
A Virtual Screening Platform Identifies Chloroethylagelastatin A as a Potential Ribosomal Inhibitor
Authors: Caulfield Thomas R. , Hayes Karen E. , Qiu Yushi , Coban Mathew , Seok Oh Joon , Lane Amy L. , Yoshimitsu Takehiko , Hazlehurst Lori , Copland John A. , Tun Han W.
Published: October 2020

Chloroethylagelastatin A (CEAA) is an analogue of agelastatin A (AA), a natural alkaloid derived from a marine sponge. It is under development for therapeutic use against brain tumors as it has excellent central nervous system (CNS) penetration and pre-clinical therapeutic activity against brain tumors. Recently, AA was shown to inhibit protein synthesis by binding to the ribosomal A-site. In this study, we developed a novel virtual screening platform to perform a comprehensive screening of various AA analogues showing that AA analogues with proven therapeutic activity including CEAA have significant ribosomal binding capacity whereas therapeutically inactive analogues show poor ribosomal binding and revealing structural fingerprint features essential for drug-ribosome interactions. In particular, CEAA was found to have greater ribosomal binding capacity than AA. Biological tests showed that CEAA binds the ribosome and contributes to protein synthesis inhibition. Our findings suggest that CEAA may possess ribosomal inhibitor activity and that our virtual screening platform may be a useful tool in discovery and development of novel ribosomal inhibitors.
Modulation of Guanylate Cyclase Activating Protein 1 (GCAP1) Dimeric Assembly by Ca2+ or Mg2+: Hints to Understand Protein Activity
Authors: Bonì Francesco , Marino Valerio , Bidoia Carlo , Mastrangelo Eloise , Barbiroli Alberto , Dell’Orco Daniele , Milani Mario
Published: October 2020

The guanylyl cyclase-activating protein 1, GCAP1, activates or inhibits retinal guanylyl cyclase (retGC) depending on cellular Ca2+ concentrations. Several point mutations of GCAP1 have been associated with impaired calcium sensitivity that eventually triggers progressive retinal degeneration. In this work, we demonstrate that the recombinant human protein presents a highly dynamic monomer-dimer equilibrium, whose dissociation constant is influenced by salt concentration and, more importantly, by protein binding to Ca2+ or Mg2+. Based on small-angle X-ray scattering data, protein-protein docking, and molecular dynamics simulations we propose two novel three-dimensional models of Ca2+-bound GCAP1 dimer. The different propensity of human GCAP1 to dimerize suggests structural differences induced by cation binding potentially involved in the regulation of retGC activity.
Metformin Enhances Excitatory Synaptic Transmission onto Hippocampal CA1 Pyramidal Neurons
Authors: Chen Wen-Bing , Chen Jiang , Liu Zi-Yang , Luo Bin , Zhou Tian , Fei Er-Kang
Published: October 2020

Metformin (Met) is a first-line drug for type 2 diabetes mellitus (T2DM). Numerous studies have shown that Met exerts beneficial effects on a variety of neurological disorders, including Alzheimer’s disease (AD), Parkinson’s disease (PD) and Huntington’s disease (HD). However, it is still largely unclear how Met acts on neurons. Here, by treating acute hippocampal slices with Met (1 μM and 10 μM) and recording synaptic transmission as well as neuronal excitability of CA1 pyramidal neurons, we found that Met treatments significantly increased the frequency of miniature excitatory postsynaptic currents (mEPSCs), but not amplitude. Neither frequency nor amplitude of miniature inhibitory postsynaptic currents (mIPSCs) were changed with Met treatments. Analysis of paired-pulse ratios (PPR) demonstrates that enhanced presynaptic glutamate release from terminals innervating CA1 hippocampal pyramidal neurons, while excitability of CA1 pyramidal neurons was not altered. Our results suggest that Met preferentially increases glutamatergic rather than GABAergic transmission in hippocampal CA1, providing a new insight on how Met acts on neurons.
Expression and Distribution Pattern of Pnn in Ischemic Cerebral Cortex and Cultured Neural Cells Exposed to Oxygen-Glucose Deprivation
Authors: Hsu Shu-Yuan , Mukda Sujira , Leu Steve
Published: October 2020

Pinin (Pnn), a multifunctional protein, participates in embryonic development as well as in cellular apoptosis, proliferation, and migration through regulating mRNA alternative splicing and gene transcription. Previous studies have shown that Pnn plays important roles in neural system development and the expression level of Pnn in astrocytes is altered by ischemic stress and associated with cellular apoptosis. In the present study, we further utilized primary cultured rat neurons and astrocytes with oxygen-glucose deprivation (OGD) and a mouse model with middle cerebral artery occlusion (MCAO)-induced ischemic stroke to examine the effect of ischemic stress on Pnn expression and distribution in different types of neural cells. Under normoxia, Pnn is mainly localized in the nuclear speckle of primary cultured neurons. The expression level of Pnn was increased after the OGD treatment and then decreased in the reoxygenation period. Moreover, the cytoplasmic expression of Pnn was observed in neurons with OGD and reoxygenation (OGD/R). Unlike that in neurons, the Pnn expression in astrocytes was decreased after OGD treatment and then gradually increased during the reoxygenation period. Of interest, the nuclear–cytoplasmic translocation of Pnn was not observed in astrocytes with OGD/R. In the MCAO mouse model, the neuronal expression of Pnn in the peri-ischemic region was reduced by three days post induction of ischemic stroke. However, the Pnn expression in astrocytes was not altered. Moreover, the nuclear speckle distribution of Pnn in neurons was also diminished following ischemic stroke. In conclusion, the Pnn expression and distribution after OGD and during reoxygenation showed distinct manners in neurons and astrocytes, implying that Pnn may play different roles in different types of neural cells in the stress response to ischemic injury.
The Role of Hypoxia and SRC Tyrosine Kinase in Glioblastoma Invasiveness and Radioresistance
Authors: Torrisi Filippo , Vicario Nunzio , Spitale Federica M. , Cammarata Francesco P. , Minafra Luigi , Salvatorelli Lucia , Russo Giorgio , Cuttone Giacomo , Valable Samuel , Gulino Rosario , Magro Gaetano , Parenti Rosalba
Published: October 2020

Advances in functional imaging are supporting neurosurgery and radiotherapy for glioblastoma, which still remains the most aggressive brain tumor with poor prognosis. The typical infiltration pattern of glioblastoma, which impedes a complete surgical resection, is coupled with a high rate of invasiveness and radioresistance, thus further limiting efficient therapy, leading to inevitable and fatal recurrences. Hypoxia is of crucial importance in gliomagenesis and, besides reducing radiotherapy efficacy, also induces cellular and molecular mediators that foster proliferation and invasion. In this review, we aimed at analyzing the biological mechanism of glioblastoma invasiveness and radioresistance in hypoxic niches of glioblastoma. We also discussed the link between hypoxia and radiation-induced radioresistance with activation of SRC proto-oncogene non-receptor tyrosine kinase, prospecting potential strategies to overcome the current limitation in glioblastoma treatment.
Progression of Pulmonary Function and Correlation with Survival Following Stereotactic Body Radiotherapy of Central and Ultracentral Lung Tumors
Authors: Regnery Sebastian , Eichkorn Tanja , Weykamp Fabian , Held Thomas , Dinges Lisa-Antonia , Schunn Fabian , Winter Hauke , Thomas Michael , Debus Jürgen , El Shafie Rami A. , Adeberg Sebastian , Hörner-Rieber Juliane
Published: October 2020

Stereotactic body radiotherapy (SBRT) to central and ultracentral lung tumors carries a risk of excessive toxicity. This study analyzed changes in pulmonary function tests (PFT) and their correlation with overall survival (OS) in 107 patients following central (n = 62) or ultracentral (n = 45) lung SBRT. Ultracentral location was defined as planning target volume overlap with the proximal bronchial tree (PBT). Vital capacity (VC) (−0.3 l, absolute −9.4% of predicted, both p < 0.001) and forced expiratory volume in the first second (FEV1s) (−0.2 l, absolute −7.7% of predicted, both p < 0.001) significantly decreased following SBRT. Higher maximum dose to the PBT significantly correlated with a steeper decline in VC (p = 0.005) and FEV1s (p = 0.03) over time. Pronounced decline in FEV1s between 6 and 12 months (HR = 0.90, p = 0.006) and pronounced decline in VC between baseline and 12 months (HR = 0.95, p = 0.004) independently correlated with worse OS. Consequently, PFT presented a statistically significant albeit clinically mild decrease in lung volumes following central and ultracentral SBRT that correlated moderately with maximum dose to the PBT. Stronger decline in pulmonary function was associated with constrained survival, advocating consequent performance of PFT during follow-up.
Photocatalytic H2 Evolution, CO2 Reduction, and NOx Oxidation by Highly Exfoliated g-C3N4
Authors: Todorova Nadia , Papailias Ilias , Giannakopoulou Tatiana , Ioannidis Nikolaos , Boukos Nikos , Dallas Panagiotis , Edelmannová Miroslava , Reli Martin , Kočí Kamila , Trapalis Christos
Published: October 2020

g-C3N4, with specific surface area up to 513 m2/g, was prepared via three successive thermal treatments at 550 °C in air with gradual precursor mass decrease. The obtained bulk and exfoliated (1ex, 2ex and 3ex) g-C3N4 were characterized and tested as photocatalysts for H2 production, CO2 reduction and NOx oxidation. The exfoliated samples demonstrated graphene-like morphology with detached (2ex) and sponge-like framework (3ex) of layers. The surface area increased drastically from 20 m2/g (bulk) to 513 m2/g (3ex). The band gap (Eg) increased gradually from 2.70 to 3.04 eV. Superoxide radicals (·O2−) were mainly formed under UV and visible light. In comparison to the bulk, the exfoliated g-C3N4 demonstrated significant increase in H2 evolution (~6 times), CO2 reduction (~3 times) and NOx oxidation (~4 times) under UV light. Despite the Eg widening, the photocatalytic performance of the exfoliated g-C3N4 under visible light was improved too. The results were related to the large surface area and low e−-h+ recombination. The highly exfoliated g-C3N4 demonstrated selectivity towards H2 evolution reactions.
Urine-Derived Epithelial Cell Lines: A New Tool to Model Fragile X Syndrome (FXS)
Authors: Zafarullah Marwa , Jasoliya Mittal , Tassone Flora
Published: October 2020

Fragile X syndrome (FXS) is an X-linked neurodevelopmental condition associated with intellectual disability and behavioral problems due to the lack of the Fragile X mental retardation protein (FMRP), which plays a crucial role in synaptic plasticity and memory. A desirable in vitro cell model to study FXS would be one that can be generated by simple isolation and culture method from a collection of a non-invasive donor specimen. Currently, the various donor-specific cells can be isolated mainly from peripheral blood and skin biopsy. However, they are somewhat invasive methods for establishing cell lines from the primary subject material. In this study, we characterized a cost-effective and straightforward method to derive epithelial cell lines from urine samples collected from participants with FXS and healthy controls (TD). The urine-derived cells expressed epithelial cell surface markers via fluorescence-activated cell sorting (FACS). We observed inter, and the intra-tissue CGG mosaicism in the PBMCs and the urine-derived cells from participants with FXS potentially related to the observed variations in the phenotypic and clinical presentation FXS. We characterized these urine-derived epithelial cells for FMR1 mRNA and FMRP expression and observed some expression in the lines derived from full mutation mosaic participants. Further, FMRP expression was localized in the cytoplasm of the urine-derived epithelial cells of healthy controls. Deficient FMRP expression was also observed in mosaic males, while, as expected, no expression was observed in cells derived from participants with a hypermethylated full mutation.
MAPLE Deposition of Binary and Ternary Organic Bulk Heterojunctions Based on Zinc Phthalocyanine
Authors: Socol Marcela , Preda Nicoleta , Petre Gabriela , Costas Andreea , Rasoga Oana , Popescu-Pelin Gianina , Mihailescu Andreea , Stanculescu Anca , Socol Gabriel
Published: October 2020

Organic bulk heterojunctions (BHJ) based on zinc phthalocyanine (ZnPc), fullerene compounds (C60 fullerene and [6,6]-phenyl C71 butyric acid methyl ester (PC70BM)), and 5,6,11,12-tetraphenylnaphthacene (rubrene) were fabricated through the matrix-assisted pulsed-laser evaporation (MAPLE) technique. Thus, ZnPc:C60 and ZnPc:PC70BM binary BHJ and ZnPc:rubrene:PC70BM ternary BHJ were deposited as thin films on various substrates. The preservation of the chemical structure of the organic compounds during the MAPLE deposition was confirmed by infrared spectroscopy. The structural, optical, and morphological properties of the deposited layers were investigated by X-ray diffraction (XRD), UV-Vis spectroscopy, photoluminescence (PL), field emission scanning electron microscopy (FESEM), and atomic force microscopy (AFM), respectively. Further, the electrical properties of the developed structures based on ZnPc:C60, ZnPc:PC70BM, and ZnPc:rubrene:PC70BM were evaluated. The J-V characteristics of the organic structures, recorded under illumination, show that an increase in the open-circuit voltage (VOC) is achieved in the case of the ternary blend in comparison with that obtained for the binary blends. The results evidenced that MAPLE-deposited thin films containing binary and ternary organic bulk heterojunctions can find applications in the field of photovoltaic devices.
Anisotropic Strain on GaN Microdisks Grown by Plasma-Assisted Molecular Beam Epitaxy
Authors: Yang Hong-Yi , Lo Ikai , Tsai Cheng-Da , Wang Ying-Chieh , Shih Huei-Jyun , Huang Hui-Chun , Chou Mitch M. C. , Huang Louie , Wang Terence , Kuo Ching T. C.
Published: October 2020

Lattice relaxation on wurtzite GaN microdisks grown by plasma-assisted molecular beam epitaxy was systematically studied. The lattice constants of GaN microdisks were evaluated from high-resolution transmission electron microscopy, and the anisotropic strain was then analyzed by observing the microscopic atomic layers. We found that the vertical lattice strain along the c-axis followed a linear relationship, while the lateral lattice strain along the a-axis exhibited a quadratic deviation. The lattice mismatch is about 0.94% at the interface between the GaN microdisks and the γ-LiAlO2 substrate, which induces the anisotropic strain during epi-growth.
Midshaft Clavicle Fractures Treated Nonoperatively Using Figure-of-Eight Bandage: Are Fracture Type, Shortening, and Displacement Radiographic Predictors of Failure?
Authors: Tagliapietra Jacopo , Belluzzi Elisa , Biz Carlo , Angelini Andrea , Fantoni Ilaria , Scioni Manuela , Bolzan Mario , Berizzi Antonio , Ruggieri Pietro
Published: October 2020

As there are no clear and unique radiographic predictors of healing disturbances for acute midshaft clavicle fractures, their treatment is still controversial. The aim of the study was to evaluate in midshaft clavicle fractures treated nonoperatively if fracture type (FT), shortening, and displacement, assessed before and after figure-of-eight bandage (F8-B) application, could be considered prognostic factors of delayed union and nonunion. One hundred twenty-two adult patients presenting a closed displaced midshaft clavicle fracture, managed nonoperatively with an F8-B, were enrolled. FT, initial shortening (IS), and initial displacement (ID) were radiographically evaluated at diagnosis, and both residual shortening (RS) and displacement (RD) were measured after F8-B application. The patients were followed up 1, 3, 6, and 12 months post-injury. Multivariate statistical analysis was performed. RD should be considered as radiological predictor of sequelae. Further, an RD equal to 104% of clavicle width was identified as an optimal cut-off point to distinguish between healed and unhealed fractures, and 140% between delayed union and nonunion. Our data pointed out the effectiveness of the F8-B in reducing fracture fragments and restoring clavicular length. In midshaft clavicle fractures of adults, fracture comminution and clavicular shortening did not influence bone healing. On the contrary, RD has been shown as the most likely predictor of both delayed union and nonunion.