bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Electrochemical Skin Conductance as a Screening Test of Cardiovascular Autonomic Neuropathy in Patients with Parkinson’s Disease
Authors: Huang Chih-Cheng , Lai Yun-Ru , Lien Chia-Yi , Cheng Ben-Chung , Tsai Nai-Wen , Lu Cheng-Hsien
Published: October 2020

Autonomic disorders have been recognized as an important non-motor feature in Parkinson’s disease (PD). However, there is a paucity of information on the presence and severity of cardiovascular autonomic neuropathy (CAN) among different motor phenotypes. The aims of this study were to examine the feasibility of electrochemical skin conductance (ESC) measured by Sudoscan as a screening service for CAN in patients with PD and investigate the severity of CAN among different motor phenotypes. Design: This was a cross-sectional observational study that enrolled 63 patients with PD. Patients were divided into three phenotypes, postural instability/gait difficulty (PIGD), tremor-dominant (TD), and akinetic-rigid (AR), according to their motor symptoms. Cardiovascular autonomic function was measured, and the presence and severity of CAN was determined according to the composite autonomic scoring scale (CASS). Functional scores were measured by the Hoehn and Yahr (HY) stage and the Unified Parkinson’s Disease Rating Scale (UPDRS). The median HY stage was 2.0 [1.5, 3.0]. Median UPDRS total score was 23.0 (17.5, 30.5), 10.0 (6.0, 11.0) and 14.0 (6.3, 23.8) in groups of PIGD, TD and AR, respectively (p = 0.001). Mean CASS was 1.7 ± 1.3, 0.6 ± 0.4, and 1.8 ± 1.5 in groups of PIGD, TD and AR, respectively (p = 0.204). Although the ESC was not strongly associated with the cardiovascular autonomic parameters, the CAN risk score provided by Sudoscan significantly correlated with parameters of cardiovascular autonomic function, including heart rate response to deep breathing (HR_DB), Valsalva ratio (VR), and baroreflex sensitivity (BRS). By receiver-operating characteristic (ROC) analysis, if a patient’s CAN risk score is higher than 33.5 (%), it is recommended to be aware of the presence of CAN even in PD patients who are asymptomatic. The area under ROC curve was 0.704. Based on our results, CAN risk score may be used for screening of CAN in patients with PD before resorting to the more sophisticated and specific, but ultimately more time-consuming, complete autonomic function testing.
Subsystem Hazard Analysis on an Offshore Waste Disposal Facility
Authors: Oh Sang-Ho , Kim Seung-Woo
Published: October 2020

Offshore waste disposal facilities are unique marine infrastructures that exist only in a few countries. Although the existing facilities in Japan and Singapore have been successfully operated in general, there have been no investigations on the probable hazards they pose on the environment. Considering this, conceivable hazards were identified for an offshore waste disposal facility that has recently been proposed in Korea. The causes and consequences of each of the identified hazards were analyzed to seek countermeasures for reducing the environmental impact in advance. Hazards of waste disposal facilities can be classified according to their design, construction, maintenance, operation, and site utilization. For these areas, except for site utilization, subsystem hazard analysis was performed. In the initial assessment, seven elements were found to be in the extreme risk zone, 30 were in the high-risk zone, and six were in the moderate-risk zone. After applying the alternative mitigation methods, the final risk assessment resulted in 27 moderate-risk and 16 low-risk elements. Therefore, it was confirmed that the potential risks of the proposed offshore waste disposal facility were within acceptable ranges.
Job Stress and Working Capacity among Fly-In-Fly-Out Workers in the Oil and Gas Extraction Industries in the Arctic
Authors: Korneeva Yana , Simonova Natalia
Published: October 2020

(1) Background: the research purpose is to identify and describe the stress and working capacity dynamics of oil and gas fly-in-fly-out (FIFO) workers in the Arctic during the fly-in period using biochemical, psychophysiological and psychological methods with further analysis of the relationship between them using objective, subjective and projective indicators. (2) Methods: The research involved 70 oil and gas FIFO specialists in the Arctic. The study of stress and working capacity was carried out using biochemical (saliva analysis for cortisol), psychophysiological (complex visual–motor reaction and variational cardiointervalometry) and psychological (questionnaire “Well-being. Activity. Mood”, M. Luscher’s color test and the subjective control level methods. (3) Results: There is a similarity in the dynamic curves of oil and gas FIFO employees’ stress and working objective, subjective and projective indicators during the fly-in period. The maximum relationships number was obtained between objective cortisol indicators in saliva (stress), complex visual–motor response indicators (operator working capacity), variational cardiointervalometry (functionality level), and interpretation coefficients (working capacity, stress, vegetative balance) according to M. Luscher’s test. (4) Conclusions: The obtained results made it possible to explain the mechanisms underlying the previously developed FIFO workers’ adaptation strategies classification, in which emergency and economic adaptation strategies were identified.
Sustaining a Transformative Disaster Risk Reduction Strategy: Grandmothers’ Telling and Singing Tsunami Stories for over 100 Years Saving Lives on Simeulue Island
Authors: Sutton Stephen A. , Paton Douglas , Buergelt Petra , Sagala Saut , Meilianda Ella
Published: October 2020

As projections about the number and scale of natural hazard events and their impact on human populations grow, increasing attention is being paid to developing effective means for preparing for and mitigating those impacts. At the same time there is an emerging understanding that gradual and incremental changes in disaster risk reduction (DRR) will not adequately meet the future needs of vulnerable populations. Transformational changes have been identified as a necessary requirement to avoid ongoing large-scale losses of life and property and models have been proposed to recalibrate DRR strategies to achieve transformative changes. One cited example of a transformative change in DRR is that of Simeulue Island. Simeulue Island suffered two tsunamis approximately 100 years apart (1907, 2004) with markedly different impacts. This paper looks in detail at the cognitive and developmental mechanisms Simeulue co-opted to sustain the transformational change throughout the 20th century. Information from interviews and observation identified the role of grandmothers have in the effective communication of risk as well as motivating appropriate action to save lives. The possibility of similarly overlooked, local, and pre-existing community capacities for transformative change in DRR is then discussed.
Comparative Analysis of Proteomes of a Number of Nosocomial Pathogens by KEGG Modules and KEGG Pathways
Authors: Slizen Mikhail V. , Galzitskaya Oxana V.
Published: October 2020

Nosocomial (hospital-acquired) infections remain a serious challenge for health systems. The reason for this lies not only in the local imperfection of medical practices and protocols. The frequency of infection with antibiotic-resistant strains of bacteria is growing every year, both in developed and developing countries. In this work, a pangenome and comparative analysis of 201 genomes of Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Mycoplasma spp. was performed on the basis of high-level functional annotations—KEGG pathways and KEGG modules. The first three organisms are serious nosocomial pathogens, often exhibiting multidrug resistance. Analysis of KEGG modules revealed methicillin resistance in 25% of S. aureus strains and resistance to carbapenems in 21% of Enterobacter spp. strains. P. aeruginosa has a wide range of unique efflux systems. One hundred percent of the analyzed strains have at least two drug resistance systems, and 75% of the strains have seven. Each of the organisms has a characteristic set of metabolic features, whose impact on drug resistance can be considered in future studies. Comparing the genomes of nosocomial pathogens with each other and with Mycoplasma genomes can expand our understanding of the versatility of certain metabolic features and mechanisms of drug resistance.
RedquorinXS Mutants with Enhanced Calcium Sensitivity and Bioluminescence Output Efficiently Report Cellular and Neuronal Network Activities
Authors: Bakayan Adil , Picaud Sandrine , Malikova Natalia P. , Tricoire Ludovic , Lambolez Bertrand , Vysotski Eugene S. , Peyriéras Nadine
Published: October 2020

Considerable efforts have been focused on shifting the wavelength of aequorin Ca2+-dependent blue bioluminescence through fusion with fluorescent proteins. This approach has notably yielded the widely used GFP-aequorin (GA) Ca2+ sensor emitting green light, and tdTomato-aequorin (Redquorin), whose bioluminescence is completely shifted to red, but whose Ca2+ sensitivity is low. In the present study, the screening of aequorin mutants generated at twenty-four amino acid positions in and around EF-hand Ca2+-binding domains resulted in the isolation of six aequorin single or double mutants (AequorinXS) in EF2, EF3, and C-terminal tail, which exhibited markedly higher Ca2+ sensitivity than wild-type aequorin in vitro. The corresponding Redquorin mutants all showed higher Ca2+ sensitivity than wild-type Redquorin, and four of them (RedquorinXS) matched the Ca2+ sensitivity of GA in vitro. RedquorinXS mutants exhibited unaltered thermostability and peak emission wavelengths. Upon stable expression in mammalian cell line, all RedquorinXS mutants reported the activation of the P2Y2 receptor by ATP with higher sensitivity and assay robustness than wt-Redquorin, and one, RedquorinXS-Q159T, outperformed GA. Finally, wide-field bioluminescence imaging in mouse neocortical slices showed that RedquorinXS-Q159T and GA similarly reported neuronal network activities elicited by the removal of extracellular Mg2+. Our results indicate that RedquorinXS-Q159T is a red light-emitting Ca2+ sensor suitable for the monitoring of intracellular signaling in a variety of applications in cells and tissues, and is a promising candidate for the transcranial monitoring of brain activities in living mice.
From Suppressor T Cells to Regulatory T Cells: How the Journey that Began with the Discovery of the Toxic Effects of TCDD Led to Better Understanding of the Role of AhR in Immunoregulation
Authors: Prasad Singh Narendra , Nagarkatti Mitzi , Nagarkatti Prakash
Published: October 2020

Aryl hydrocarbon receptor (AhR) was identified in the early 1970s as a receptor for the ubiquitous environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin), which is a member of halogenated aromatic hydrocarbons (HAHs). TCDD was found to be highly toxic to the immune system, causing thymic involution and suppression of a variety of T and B cell responses. The fact that environmental chemicals cause immunosuppression led to the emergence of a new field, immunotoxicology. While studies carried out in early 1980s demonstrated that TCDD induces suppressor T cells that attenuate the immune response to antigens, further studies on these cells were abandoned due to a lack of specific markers to identify such cells. Thus, it was not until 2001 when FoxP3 was identified as a master regulator of Regulatory T cells (Tregs) that the effect of AhR activation on immunoregulation was rekindled. The more recent research on AhR has led to the emergence of AhR as not only an environmental sensor but also as a key regulator of immune response, especially the differentiation of Tregs vs. Th17 cells, by a variety of endogenous, microbial, dietary, and environmental ligands. This review not only discusses how the role of AhR emerged from it being an environmental sensor to become a key immunoregulator, but also confers the identification of new AhR ligands, which are providing novel insights into the mechanisms of Treg vs. Th17 differentiation. Lastly, we discuss how AhR ligands can trigger epigenetic pathways, which may provide new opportunities to regulate inflammation and treat autoimmune diseases.
Surface Wiping Test to Study Biocide -Cinnamaldehyde Combination to Improve Efficiency in Surface Disinfection
Authors: Malheiro Joana F. , Oliveira Catarina , Cagide Fernando , Borges Fernanda , Simões Manuel , Maillard Jean-Yves
Published: October 2020

Disinfection is crucial to control and prevent microbial pathogens on surfaces. Nonetheless, disinfectants misuse in routine disinfection has increased the concern on their impact on bacterial resistance and cross-resistance. This work aims to develop a formulation for surface disinfection based on the combination of a natural product, cinnamaldehyde, and a widely used biocide, cetyltrimethylammonium bromide. The wiping method was based on the Wiperator test (ASTM E2967−15) and the efficacy evaluation of surface disinfection wipes test (EN 16615:2015). After formulation optimization, the wiping of a contaminated surface with 6.24 log10 colony-forming units (CFU) of Escherichia coli or 7.10 log10 CFU of Staphylococcus aureus led to a reduction of 4.35 log10 CFU and 4.27 log10 CFU when the wipe was impregnated with the formulation in comparison with 2.45 log10 CFU and 1.50 log10 CFU as a result of mechanical action only for E. coli and S. aureus, respectively. Furthermore, the formulation prevented the transfer of bacteria to clean surfaces. The work presented highlights the potential of a combinatorial approach of a classic biocide with a phytochemical for the development of disinfectant formulations, with the advantage of reducing the concentration of synthetic biocides, which reduces the potentially negative environmental and public health impacts from their routine use.
Phe-140 Determines the Catalytic Efficiency of Arylacetonitrilase from Alcaligenes faecalis
Authors: Kim Jung-Soo , Patel Sanjay K. S. , Tiwari Manish K. , Lai Chunfen , Kumar Anurag , Kim Young Sin , Kalia Vipin Chandra , Lee Jung-Kul
Published: October 2020

Arylacetonitrilase from Alcaligenes faecalis ATCC8750 (NitAF) hydrolyzes various arylacetonitriles to the corresponding carboxylic acids. A systematic strategy of amino acid residue screening through sequence alignment, followed by homology modeling and biochemical confirmation was employed to elucidate the determinant of NitAF catalytic efficiency. Substituting Phe-140 in NitAF (wild-type) to Trp did not change the catalytic efficiency toward phenylacetonitrile, an arylacetonitrile. The mutants with nonpolar aliphatic amino acids (Ala, Gly, Leu, or Val) at location 140 had lower activity, and those with charged amino acids (Asp, Glu, or Arg) exhibited nearly no activity for phenylacetonitrile. Molecular modeling showed that the hydrophobic benzene ring at position 140 supports a mechanism in which the thiol group of Cys-163 carries out a nucleophilic attack on a cyanocarbon of the substrate. Characterization of the role of the Phe-140 residue demonstrated the molecular determinant for the efficient formation of arylcarboxylic acids.
Increased Expression of TLR4 in Circulating CD4+T Cells in Patients with Allergic Conjunctivitis and In Vitro Attenuation of Th2 Inflammatory Response by Alpha-MSH
Authors: Nieto Jane E. , Casanova Israel , Serna-Ojeda Juan Carlos , Graue-Hernández Enrique O. , Quintana Guillermo , Salazar Alberto , Jiménez-Martinez María C.
Published: October 2020

Ocular allergic diseases are frequently seen in ophthalmological clinical practice. Immunological damage is mediated by a local Th2 inflammatory microenvironment, accompanied by changes in circulating cell subsets, with more effector cells and fewer T regulatory cells (Tregs). This study aimed to evaluate the involvement of toll-like receptor 4 (TLR4) and α-melanocyte stimulating hormone (α-MSH) in the immune regulation associated with perennial allergic conjunctivitis (PAC). We performed an Ag-specific stimulation during 72 h of culturing with or without lipopolysaccharide (LPS) or α-MSH in peripheral blood mononuclear cells (PBMC), analyzing the cell subsets and cytokines induced by the stimuli. We also determined α-MSH in tear samples from healthy donors (HD) or PAC patients. Our findings demonstrate an immunological dysregulation characterized by an increased frequency of CD4+TLR4+ in the PBMC of patients with PAC, compared to HD. Most of these CD4+TLR4+ cells were also CD25+, and when α-MSH was added to the culture, the percentage of CD4+CD25+FoxP3+ increased significantly, while the percentage of CD69+ cells and cytokines IL-4 and IL-6 were significantly decreased. In tears, we found an increased concentration of α-MSH in PAC patients, compared with HD. These findings indicate a novel mechanism involved in controlling ocular allergic diseases, in which α-MSH diminishes the concentration of IL-6 and IL-4, restoring the frequency of Tregs and down-regulating CD4 activation. Moreover, we demonstrated the involvement of CD4+TLR4+ cells as an effector cell subset in ocular allergy.
Mapping a Circular RNA–microRNA–mRNA-Signaling Regulatory Axis that Modulates Stemness Properties of Cancer Stem Cell Populations in Colorectal Cancer Spheroid Cells
Authors: Rengganaten Vimalan , Huang Chiu-Jung , Tsai Ping-Hsing , Wang Mong-Lien , Yang Yi-Ping , Lan Yuan-Tzu , Fang Wen-Liang , Soo Shelly , Ong Hooi Tin , Cheong Soon Keng , Choo Kong Bung , Chiou Shih-Hwa
Published: October 2020

Spheroidal cancer cell cultures have been used to enrich cancer stem cells (CSC), which are thought to contribute to important clinical features of tumors. This study aimed to map the regulatory networks driven by circular RNAs (circRNAs) in CSC-enriched colorectal cancer (CRC) spheroid cells. The spheroid cells established from two CRC cell lines acquired stemness properties in pluripotency gene expression and multi-lineage differentiation capacity. Genome-wide sequencing identified 1503 and 636 circRNAs specific to the CRC parental and spheroid cells, respectively. In the CRC spheroids, algorithmic analyses unveiled a core network of mRNAs involved in modulating stemness-associated signaling pathways, driven by a circRNA–microRNA (miRNA)–mRNA axis. The two major circRNAs, hsa_circ_0066631 and hsa_circ_0082096, in this network were significantly up-regulated in expression levels in the spheroid cells. The two circRNAs were predicted to target and were experimentally shown to down-regulate miR-140-3p, miR-224, miR-382, miR-548c-3p and miR-579, confirming circRNA sponging of the targeted miRNAs. Furthermore, the affected miRNAs were demonstrated to inhibit degradation of six mRNA targets, viz. ACVR1C/ALK7, FZD3, IL6ST/GP130, SKIL/SNON, SMAD2 and WNT5, in the CRC spheroid cells. These mRNAs encode proteins that are reported to variously regulate the GP130/Stat, Activin/Nodal, TGF-β/SMAD or Wnt/β-catenin signaling pathways in controlling various aspects of CSC stemness. Using the CRC spheroid cell model, the novel circRNA–miRNA–mRNA axis mapped in this work forms the foundation for the elucidation of the molecular mechanisms of the complex cellular and biochemical processes that determine CSC stemness properties of cancer cells, and possibly for designing therapeutic strategies for CRC treatment by targeting CSC.
Printed Graphene Layer as a Base for Cell Electrostimulation—Preliminary Results
Authors: Dybowska-Sarapuk Lucja , Sosnowicz Weronika , Krzeminski Jakub , Grzeczkowicz Anna , Granicka Ludomira H. , Kotela Andrzej , Jakubowska Malgorzata
Published: October 2020

Nerve regeneration through cell electrostimulation will become a key finding in regenerative medicine. The procedure will provide a wide range of applications, especially in body reconstruction, artificial organs or nerve prostheses. Other than in the case of the conventional polystyrene substrates, the application of the current flow in the cell substrate stimulates the cell growth and mobility, supports the synaptogenesis, and increases the average length of neuron nerve fibres. The indirect electrical cell stimulation requires a non-toxic, highly electrically conductive substrate material enabling a precise and effective cell electrostimulation. The process can be successfully performed with the use of the graphene nanoplatelets (GNPs)—the structures of high conductivity and biocompatible with mammalian NE-4C neural stem cells used in the study. One of the complications with the production of inks using GNPs is their agglomeration, which significantly hampers the quality of the produced coatings. Therefore, the selection of the proper amount of the surfactant is paramount to achieve a high-quality substrate. The article presents the results of the research into the material manufacturing used in the cell electrostimulation. The outcomes allow for the establishment of the proper amount of the surfactant to achieve both high conductivity and quality of the coating, which could be used not only in electronics, but also—due to its biocompatibility—fruitfully applied to the cell electrostimulation.
Doxorubicin Improves Cancer Cell Targeting by Filamentous Phage Gene Delivery Vectors
Authors: Tsafa Effrosyni , Bentayebi Kaoutar , Topanurak Supachai , Yata Teerapong , Przystal Justyna , Fongmoon Duriya , Hajji Nabil , Waramit Sajee , Suwan Keittisak , Hajitou Amin
Published: October 2020

Merging targeted systemic gene delivery and systemic chemotherapy against cancer, chemovirotherapy, has the potential to improve chemotherapy and gene therapy treatments and overcome cancer resistance. We introduced a bacteriophage (phage) vector, named human adeno-associated virus (AAV)/phage or AAVP, for the systemic targeting of therapeutic genes to cancer. The vector was designed as a hybrid between a recombinant adeno-associated virus genome (rAAV) and a filamentous phage capsid. To achieve tumor targeting, we displayed on the phage capsid the double-cyclic CDCRGDCFC (RGD4C) ligand that binds the alpha-V/beta-3 (αvβ3) integrin receptor. Here, we investigated a combination of doxorubicin chemotherapeutic drug and targeted gene delivery by the RGD4C/AAVP vector. Firstly, we showed that doxorubicin boosts transgene expression from the RGD4C/AAVP in two-dimensional (2D) cell cultures and three-dimensional (3D) tumor spheres established from human and murine cancer cells, while preserving selective gene delivery by RGD4C/AAVP. Next, we confirmed that doxorubicin does not increase vector attachment to cancer cells nor vector cell entry. In contrast, doxorubicin may alter the intracellular trafficking of the vector by facilitating nuclear accumulation of the RGD4C/AAVP genome through destabilization of the nuclear membrane. Finally, a combination of doxorubicin and RGD4C/AAVP-targeted suicide gene therapy exerts a synergistic effect to destroy human and murine tumor cells in 2D and 3D tumor sphere settings.
Beyond Hydrogen Storage—Metal Hydrides as Multifunctional Materials for Energy Storage and Conversion
Authors: Møller Kasper T. , Sargent Anna-Lisa , Remhof Arndt , Heere Michael
Published: October 2020

Following the E-MRS (European Materials Research Society) fall meeting 2019, Symposium L, this Special Issue of Inorganics, entitled “Beyond Hydrogen Storage—Metal Hydrides as Multifunctional Materials for Energy Storage and Conversion”, is dedicated to the wide range of emerging energy-related inorganic hydrogen-containing materials [...]
Phytohormone Cross-Talk Synthesizes Glycoalkaloids in Potato (Solanum tuberosum L.) in Response to Aphid (Myzus persicae Sulzer) Infestation under Drought Stress
Authors: Quandahor Peter , Gou Yuping , Lin Chunyan , Mujitaba Dawuda Mohammed , A. Coulter Jeffrey , Liu Changzhong
Published: October 2020

Potato production is adversely affected by aphid infestation across the globe. Understanding the mechanism of host plant defense against aphids under drought stress is paramount for insect pest management. This study was conducted to examine the cross-talk of phytohormones in potato glycoalkaloids’ defense against green peach aphids under greenhouse conditions. A 3 × 2 × 2 factorial experiment comprising three potato cultivars (Qingshu 9, Longshu 3, and Atlantic) and two levels each of water availability and aphid infestation was conducted. The results show that under drought stress, green peach aphids thrive well on host plants, which contain a relatively high water content. The resistant cultivar DXY, which exhibited a higher level of phytohormones, also demonstrated higher α-chaconine and α-solanine contents in both leaf and root, under drought and aphid stress. Conversely, the susceptible cultivar QS9, which exhibited a lower level of phytohormones, also demonstrated low α-chaconine and α-solanine contents in both leaf and root, under drought and aphid stress. The DXY cultivar, which possessed high resistant traits such as α-chaconine and α-solanine, can be used in areas where green peach aphid infestation is a major setback.
Activity of Ajuga iva Extracts Against the African Cotton Leafworm Spodoptera littoralis
Authors: Taha-Salaime Leena , Lebedev Galina , Abo-Nassar Jackline , Marzouk Sally , Inbar Moshe , Ghanim Murad , Aly Radi
Published: October 2020

Control of the crop pest African cotton leafworm, Spodoptera littoralis (Boisduval), by chemical insecticides has led to serious resistance problems. Ajuga plants contain phytoecdysteroids (arthropod steroid hormone analogs regulating metamorphosis) and clerodanes (diterpenoids exhibiting antifeedant activity). We analyzed these compounds in leaf extracts of the Israeli Ajuga iva L. by liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) and thin-layer chromatography (TLC), and their efficiency at reducing S.littoralis fitness. First and third instars of S. littoralis were fed castor bean leaves (Ricinus communis) smeared with an aqueous suspension of dried methanolic crude extract of A. iva phytoecdysteroids and clerodanes. Mortality, larval weight gain, relative growth rate and survival were compared to feeding on control leaves. We used ‘4’,6-diamidino-2-phenylindole (DAPI, a fluorescent stain) and phalloidin staining to localize A. iva crude leaf extract activity in the insect gut. Ajuga iva crude leaf extract (50, 100 and 250 µg/µL) significantly increased mortality of first-instar S. littoralis (36%, 70%, and 87%, respectively) compared to controls (6%). Third-instar larval weight gain decreased significantly (by 52%, 44% and 30%, respectively), as did relative growth rate (−0.05 g/g per day compared to the relevant controls), ultimately resulting in few survivors. Crude leaf extract (250 µg/µL) reduced gut size, with relocation of nuclei and abnormal actin-filament organization. Ajug iva extract has potential for alternative, environmentally safe insect-pest control.
Measuring the Electrical and Photonic Properties of Cobalt Oxide-Containing Composite Carbon Fibers
Authors: Gan Yong X. , Gan Jeremy B.
Published: October 2020

In this work, cobalt acetate was incorporated into polyacrylonitrile (PAN) polymer through electrospinning as the cobalt oxide source. After oxidization and pyrolysis, a PAN-derived composite carbon fiber containing cobalt oxide was obtained. Measuring the electrical and photonic properties of the composite fiber under visible light irradiation was performed to evaluate the photoelectric behavior of the composite fiber. The p-type semiconducting behavior of the composite fiber was confirmed by measuring the open circuit voltage of a photochemical fuel cell consisting of the photosensitive electrode made from the composite fiber. The application of the composite fiber for glucose sensing was demonstrated.
The Capabilities of Spark-Assisted Chemical Engraving: A Review
Authors: Bassyouni Zahraa , Abou Ziki Jana
Published: October 2020

Brittle non-conductive materials, like glass and ceramics, are becoming ever more significant with the rising demand for fabricating micro-devices with special micro-features. Spark-Assisted Chemical Engraving (SACE), a novel micromachining technology, has offered good machining capabilities for glass and ceramic materials in basic machining operations like drilling, milling, cutting, die sinking, and others. This paper presents a review about SACE technology. It highlights the process fundamentals of operation and the key machining parameters that control it which are mainly related to the electrolyte, tool-electrode, and machining voltage. It provides information about the gas film that forms around the tool during the process and the parameters that enhance its stability, which play a key role in enhancing the machining outcome. This work also presents the capabilities and limitations of SACE through comparing it with other existing micro-drilling and micromachining technologies. Information was collected regarding micro-channel machining capabilities for SACE and other techniques that fall under four major glass micromachining categories—mainly thermal, chemical, mechanical, and hybrid. Based on this, a figure that presents the capabilities of such technologies from the perspective of the machining speed (lateral) and resulting micro-channel geometry (aspect ratio) was plotted. For both drilling and micro-channel machining, SACE showed to be a promising technique compared to others as it requires relatively cheap set-up, results in high aspect ratio structures (above 10), and takes a relatively short machining time. This technique shows its suitability for rapid prototyping of glass micro-parts and devices. The paper also addresses the topic of surface functionalization, specifically the surface texturing done during SACE and other glass micromachining technologies. Through tuning machining parameters, like the electrolyte viscosity, tool–substrate gap, tool travel speed, and machining voltage, SACE shows a promising and unique potential in controlling the surface properties and surface texture while machining.
Effect of Host, Environment and Fungal Growth on Fungal Leaf Endophyte Communities in Taiwan
Authors: Huang Yu-Ling
Published: October 2020

Fungal endophytes inhabit plant tissues without causing disease symptoms. They are highly diverse and distributed globally in all plants that have been investigated. Host, geographic, and environmental effects on endophyte communities have been reported in several studies, but the direct effect of fungal growth rate on endophyte composition has not been tested. To understand the relationship between foliar endophyte composition and fungal growth and to examine the effect of host, elevation, and climatic factors on the foliar endophyte communities, this study examined the foliar endophyte communities of representative gymnosperms and Rhododendron spp. across different elevations of Hehuanshan and Taipingshan forests in Taiwan. The isolation frequency and diversity of foliar endophytes were higher at low elevations than at high elevations. The foliar endophyte community structure differed as a function of host family and forest vegetation type. Elevation, mean annual temperature, and precipitation were significantly correlated with the community structure. Fungal growth rate was correlated with the endophyte abundance, which indicates that fast-growing fungi might have a competitive advantage when coexisting with other fungi in a plant host.
Tool Path Design of the Counter Single Point Incremental Forming Process to Decrease Shape Error
Authors: Jung Kyu-Seok , Yu Jae-Hyeong , Chung Wan-Jin , Lee Chang-Whan
Published: October 2020

Incremental sheet metal forming can manufacture various sheet metal products without a dedicated punch and die set. In this study, we developed a two-stage incremental forming process to decrease shape errors in the conventional incremental forming process. The forming process was classified into the first single point incremental forming (1st SPIF) process for forming a product and the counter single point incremental forming (counter SPIF) process to decrease shape error. The counter SPIF gives bending deformation in the opposite direction. Furthermore, the counter SPIF compensates for shape errors, such as section deflection, skirt spring-back, final forming height, and round. The tool path of the counter SPIF has been optimized through a relatively simple optimization method by modifying the tool path of the previous step. The tool path of the 1st SPIF depends on the geometry of the product. An experiment was performed to form a circular cup shape to verify the proposed tool path of the 1st and counter SPIF. The result confirmed that the shape error decreased when compared to the conventional SPIF. For the application, the ship-hull geometry was adopted. Experimental results demonstrated the feasibility of the two-stage incremental forming process.
Hydrogen Sulfide Adsorption by Iron Oxides and Their Polymer Composites: A Case-Study Application to Biogas Purification
Authors: Costa Camilla , Cornacchia Matteo , Pagliero Marcello , Fabiano Bruno , Vocciante Marco , Reverberi Andrea Pietro
Published: October 2020

An experimental study of hydrogen sulfide adsorption on a fixed bed for biogas purification is proposed. The adsorbent investigated was powdered hematite, synthesized by a wet-chemical precipitation method and further activated with copper (II) oxide, used both as produced and after pelletization with polyvinyl alcohol as a binder. The pelletization procedure aims at optimizing the mechanical properties of the pellet without reducing the specific surface area. The active substrate has been characterized in its chemical composition and physical properties by X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM), thermogravimetric analysis (TGA) and N2 physisorption/desorption for the determination of surface area. Both powders and pellets have been tested as sorbents for biogas purification in a fixed bed of a steady-state adsorption column and the relevant breakthrough curves were determined for different operating conditions. The performance was critically analyzed and compared with that typical of other commercial sorbents based on zinc oxide or relying upon specific compounds supported on a chemically inert matrix (SulfaTreat®). The technique proposed may represent a cost-effective and sustainable alternative to commercial sorbents in conventional desulphurization processes.
Epitaxial Growth of Silicon on Silicon Wafers by Direct Laser Melting
Authors: Le Dantec Marie , Abdulstaar Mustafa , Leparoux Marc , Hoffmann Patrik
Published: October 2020

Additive manufacturing (AM) of brittle materials remains challenging, as they are prone to cracking due to the steep thermal gradients present during melting and cooling after laser exposition. Silicon is an ideal brittle material for study since most of the physical properties of single-element materials can be found in the literature and high-purity silicon powders are readily available. Direct laser melting (DLM) of silicon powder was performed to establish the conditions under which cracks occur and to understand how the solidification front impacts the final microstructure. Through careful control of process conditions, paying special attention to thermal gradients and the growth velocity, epitaxial pillars free of cracks could be grown to a length of several millimeters.
Strength-Ductility Synergy in a Metastable β Titanium Alloy by Stress Induced Interfacial Twin Boundary ω Phase at Cryogenic Temperatures
Authors: Li Yongkang , Liao Zhibin , Zhang Weidong , Wu Zhenggang , Zhou Canxu
Published: October 2020

A β titanium alloy is an excellent candidate for cryogenic applications. In this study, the deformation behavior of Ti-36Nb-2Ta-3Zr-0.35O with cold swaging was investigated at cryogenic temperatures to verify its practical application value. The microstructure after tensile tests was observed by transmission electron microscope in order to reveal the cryogenic deformation mechanism. The results show that the mechanical properties of this alloy have a strong temperature dependence: an increase in strength with a non-monotonic trend (first increase and then decrease) in elongation is found when the temperature decreases from 297 K to 77 K. At 200 K, a strength-ductility synergy is obtained and is mainly due to the occurrence of {211} <11> mechanical twinning accompanied with the ω plate located at the twin boundaries, which is the first time it is detected in titanium alloy at a cryogenic temperature. However, at 77 K, martensitic transformation (β phase to α phase) is induced by the tensile deformation, leading to the increase of strength with a massive sacrifice of elongation. These findings provide insights for understanding the deformation mechanisms and optimizing the mechanical properties of titanium alloys at a cryogenic temperature.
Calcined Clay as Supplementary Cementitious Material
Authors: Jaskulski Roman , Jóźwiak-Niedźwiedzka Daria , Yakymechko Yaroslav
Published: October 2020

Calcined clays are the only potential materials available in large quantities to meet the requirements of eco-efficient cement-based materials by reducing the clinker content in blended cements or reducing the cement content in concrete. More than 200 recent research papers on the idea of replacing Portland cement with large amounts of calcined clay are presented and discussed in detail. First, the fundamental information about the properties and structure of clay minerals is described. Then, the process of activation and hydration of clays is discussed, including the methods of pozzolanic activity assessment. Additionally, various testing methods of clays from different worldwide deposits are presented. The application of calcined clay in cement and concrete technology is then introduced. A separate chapter is devoted to lime calcined clay cement. Then an influence of calcined clay on durability of concrete is summarized. Finally, conclusions are formulated.
Electromechanical Impedance Model for Free 1D Thin-Walled Piezoelectric Ceramics with a Novel Derivation
Authors: Kong Xiangxing , Chen Chunyang , Liu Xiao , Zhu Jianjian , Qing Xinlin
Published: October 2020

The electromechanical impedance model of the piezoelectric ceramics in a free state can be used for screening and quality control in the structural health monitoring community, but the derivation process of the existing model is usually complicated. This paper describes a novel theoretical derivation methodology based on the assumption of zero-stress on the free boundary of the one-dimensional transducer, which can simplify the derivation of the model to a large extent. To assess the accuracy of the model, a signal processing method based on frequency shifting transformation and the Pearson correlation coefficient is also proposed to calculate the similarity between theoretically predicted and experimentally measured data. Two different piezoelectric ceramics were used in experiments to verify the effectiveness of the model. Experimental results convincingly demonstrate that the assumption proposed in this paper possesses good feasibility for one-dimensional thin-walled piezoelectric ceramics and the model has excellent precision.
Casting and Solidification of Light Alloys
Authors: Vorozhtsov Alexander
Published: October 2020

At present, light alloys based on aluminum, magnesium, titanium, etc [...]
Effects of Low and High FODMAP Diets on Human Gastrointestinal Microbiota Composition in Adults with Intestinal Diseases: A Systematic Review
Authors: Vandeputte Doris , Joossens Marie
Published: October 2020

A diet high in non-digestible carbohydrates is known to promote health, in part through its effect on the gut microbiome. While substantially proven for healthy individuals, these effects are more ambiguous in subjects with intestinal diseases. At the same time, a diet low in these fermentable carbohydrates, the low FODMAP (acronym for Fermentable Oligo-, Di-, Mono-saccharides, And Polyols) diet, is gaining popularity as a treatment option for symptom relief in irritable bowel syndrome and inflammatory bowel disease. There are, however, several indications that this diet induces effects opposite to those of prebiotic supplementation, resulting in gut microbiome changes that might be detrimental. Here, we provide a systematic review of the effects of low and high FODMAP diets on human gastrointestinal microbiota composition in adults with intestinal diseases, through literature screening using the databases PubMed, Embase, and Web of Science. We summarize study findings on dietary impact in patients, including the effect on bacterial taxa and diversity. In general, similar to healthy subjects, restricting non-digestible carbohydrate intake in patients with intestinal diseases has opposite effects compared to prebiotic supplementation, causing a reduction in bifidobacteria and an increase in bacteria associated with dysbiosis. Future studies should focus on assessing whether the induced microbial changes persist over time and have adverse effects on long-term colonic health.
Phenolic Acids-Rich Fractions from Agaricus bitorguis (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells
Authors: Lu Hongyun , Jiao Zhihua , Jiao Yingchun , Wang Wei , Chen Qihe
Published: October 2020

Hypoxia is a common pathological process in various clinical diseases. However, there is still a lack of effective anti-hypoxia active substances. Agaricus bitorguis (Quél.) Sacc Chaidam (ABSC) is a rare wild edible macrofungus that grows underground at high altitudes. Herein, intracellular phenolic acids-rich fractions (IPA) were extracted from ABSC ZJU-CDMA-12, and the structural characterization and anti-hypoxia activity of IPA on PC12 cells were elucidated as well. The results of HPLC-Q-TOF-MS illustrated that five kinds of IPA were isolated from ABSC, including (−)-epicatechin gallate, arabelline, yunnaneic acid D, 2′-O-p-hydroxybenzoyl-6′-O-trans-caffeoylgardoside,4′-O-methylgallocatechin-(4->8)-4′-O-methylepigallocatechin. IPA extracted from ABSC proved to show anti-hypoxia activity on hypoxia-damaged PC12 cells. Hypoxia enhanced reactive oxygen species (ROS) generation and reduced the mitochondrial membrane potential (ΔΨm) in PC12 cells, resulting in the inhibition of survival and induction of apoptosis in PC12 cells. Measurements of 100 μg/mL and 250 μg/mL IPA could significantly reduce hypoxia-induced damage in PC12 cells by decreasing overproduced intracellular ROS, improving ΔΨm, and reducing cell apoptosis rate. Our findings indicated that the IPA from ABSC potentially could be used as novel bioactive components applied to anti-hypoxia functional foods or medicines.
Micro-Fibrillated Cellulose in Adhesive Systems for the Production of Wood-Based Panels
Authors: Karagiannidis Emmanouil , Markessini Charles , Athanassiadou Eleftheria
Published: October 2020

Micro-Fibrillated Cellulose (MFC) is a new type of bio-based additive, coming from wood cellulose. It can compete and substitute oil derived chemicals in several application fields. In the present work, the use of micro-fibrillated cellulose, in waterborne adhesive systems applied in the manufacture of composite wood-based panels was evaluated. Research was conducted to test the potential of improving the performance of wood-based panel types such as particleboard, waferboard or randomly-oriented strand board and plywood, by the application of MFC and the substitution of conventional and non-renewable chemical compounds. The approaches followed to introduce MFC into the adhesive systems were three, i.e., MFC 2% suspension added during the adhesive resin synthesis, MFC 10% paste admixed with the already prepared adhesive resin and MFC 2% suspension admixed with the already prepared resin. It was found that MFC improves not only the performance of the final wood panel products but also the behaviour of the applied adhesive polymer colloids (e.g., rheology improvement), especially when admixed with the already prepared resins. Moreover, it was proven that when MFC is introduced into the adhesive resin system, there is a possibility of decreasing the resin consumption, by maintaining the board performance. MFC’s robustness to pH, shear and temperature makes it a highly interesting new additive for adhesive producers. In addition, its natural origin can give adhesive producers the opportunity to move over to more environmentally friendly product solutions.
Total Synthesis of Phorbazole B
Authors: Guttormsen Yngve , Fairhurst Magnus E. , Pandey Sunil K. , Isaksson Johan , Haug Bengt Erik , Bayer Annette
Published: October 2020

Phorbazoles are polychlorinated heterocyclic secondary metabolites isolated from a marine sponge and several of these natural products have shown inhibitory activity against cancer cells. In this work, a synthesis of the trichlorinated phorbazole B using late stage electrophilic chlorination was developed. The synthesis relied on the use of an oxazole precursor, which was protected with an iodine in the reactive 4-position, followed by complete chlorination of all pyrrole positions. Attempts to prepare phorbazole A and C, which contain a 3,4-dichlorinated pyrrole, were unsuccessful as the desired chlorination pattern on the pyrrole could not be obtained. The identities of the dichlorinated intermediates and products were determined using NMR techniques including NOESY/ROESY, 1,1-ADEQUATE and high-resolution CLIP-HSQMBC.
Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma
Authors: Huang Cheng-Chen , Wang Po-Hui , Lu Yen-Ting , Yang Jia-Sin , Yang Shun-Fa , Ho Yu-Ting , Lin Chiao-Wen , Hsin Chung-Han
Published: October 2020

The most important cause of treatment failure of nasopharyngeal carcinoma (NPC) patients is metastasis, including regional lymph nodes or distant metastasis, resulting in a poor prognosis and challenges for treatment. In the present study, we investigated the in vitro anti- tumoral properties of morusin on human nasopharyngeal carcinoma HONE-1, NPC-39, and NPC-BM cells. Our study revealed that morusin suppressed the migration and invasion abilities of the three NPC cells. Gelatin zymography assay and Western blotting demonstrated that the enzyme activity and the level of matrix metalloproteinases-2 (MMP-2) protein were downregulated by the treatment of morusin. Mitogen-activated protein kinase proteins were examined to identify the signaling pathway, which showed that phosphorylation of ERK1/2 was inhibited after the treatment of morusin. In summary, our data showed that morusin inhibited the migration and invasion of NPC cells by suppressing the expression of MMP-2 by downregulating the ERK1/2 signaling pathway, suggesting that morusin may be a potential candidate for chemoprevention or adjuvant therapy of NPC.