bluuebunny/crossref_metadata_2025 huggingface dataset:
Is it possible to diagnose therapeutic adherence in mild cognitive impairment and dementia patients in clinical practice?
Authors: Barnestein-Fonseca Pilar , Guerrero-Pertiñez Gloria , Gúzman-Parra Jose , Valera-Moreno Esperanza , Mayoral-Cleries Fermín
Published: May 2024

Background

Non-adherence is common and contributes to adverse health outcomes, reduced quality of life, and increased healthcare expenditure. The objective of this study was to assess the diagnostic validity to estimate the prevalence of non-adherence in patients with mild cognitive impairment (MCI) and dementia using two self-reported methods (SRMs) that are useful and easy in clinical practice, considering the pill count as a reference method (RM).

Methods

The cohort study was nested in a multicenter randomized controlled trial NCT03325699. A total of 387 patients from 8 health centers were selected using a non-probabilistic consecutive sampling method. Inclusion criteria were as follows: a score of 20–28 points on the Mini-Mental State Examination (MMSE); older than 55 years; taking prescribed medication; and are in charge of their own medication use. Participants were followed up for 18 months after the baseline visit, i.e., 6, 12, and 18 months. Variables related with treatment adherences were measured in all visits. The variables included age, sex, treatment, comorbidities, and the MMSE test. Adherences included pill counts and Morisky–Green test (MGT) and Batalla test (BT) as SRMs. Statistical analysis included descriptive analysis and 95% confidence intervals (CIs). The diagnostic validity included the following: 1) open comparison statistical association between SRMs and RMs and 2) hierarchy comparison: the RM as the best method to assess non-adherence, kappa value (k), sensitivity (S), specificity (Sp), and likelihood ratio (PPV/PPN).

Results

A total of 387 patients were recruited with an average age of 73.29 years (95% CI, 72.54–74.04), of which 59.5% were female. Comorbidities were 54.4% HTA, 35.9% osteoarticular pathology, and 24.5% DM. The MMSE mean score was 25.57 (95% CI, 25.34–25.8). The treatment adherence for the RM oscillates between 22.5% in the baseline and 26.3%, 14.8%, and 17.9% in the follow-up visits. For SRMs, the treatment adherence oscillates between 43.5% in the baseline and 32.4%, 21.9%, and 20.3% in the follow-up visits. The kappa value was statistically significant in all the comparison in all visits with a score between 0.16 and 035. Regarding the diagnostic validity, for the MGT, the sensibility oscillated between 0.4 and 0.58, and the specificity oscillated between 0.68 and 0.87; for the BT, the sensibility oscillated between 0.4 and 0.7, and the specificity oscillated between 0.66 and 0.9; and when both tests were used together, the sensibility oscillated between 0.22 and 0.4, and the specificity oscillated between 0.85 and 0.96.

Conclusion

SRMs classify non-adherent subjects correctly. They are very easy to use and yield quick results in clinical practice, so SRMs would be used for the non-adherence diagnosis in patients with MCI and mild dementia.
Current use of medicinal plants for children’s diseases among mothers in Southern Romania
Authors: Petran Madalina , Dragoș Dorin , Stoian Irina , Vlad Adelina , Gilca Marilena
Published: May 2024

There is a limited number of studies focusing on ethnomedical practices in children, particularly in Eastern Europe. Romania has a rich history of using medicinal plants in ethnopediatric care, and our objective was to identify the medicinal plants currently employed in treating childhood illnesses in the southern region of the country.

Material and methods Our investigation used structured interviews, focusing on respondent demographics, local names of therapeutically employed herbs, the specific plant part(s) utilized, methods of preparation and administration, and local folk indications of taxa. A total of 326 mothers with children aged 0 to 18, hospitalized in the “Grigore Alexandrescu” Children Emergency Hospital Bucharest and residing in Southern Romania, were enrolled in the study. Use Value Citation Index (UVc), Informant Consensus Factor (Fic), and Fidelity Level (FL) were calculated.

Results Twenty-five plants were identified for treating children’s diseases in Southern Romania. The majority of informants resided in urban areas, and mothers primarily acquired knowledge from family members and healthcare professionals. The herbs most frequently employed were Mentha spp. (UV = 0.509) for diarrhea, Matricaria spp. (UV = 0.301) for skin infections (Fic = 0.99) and digestive diseases (Fic = 0.98), and Calendula officinalis L. (UV = 0.365) for skin diseases (Fic = 0.99). Less utilized were Raphanus raphanistrum subsp. sativus (L.) Domin in respiratory diseases, Prunus avium (L.) L. stalks in urinary tract ailments, Helianthus annuus L. in ear infections, Allium sativum L. in intestinal parasitosis, Viola tricolor L. in hives, Triticum aestivum L. in dermatitis and Allium ursinum L. as a tonic. In 184 cases herbal treatment was used in conjunction with conventional medications. Education level correlated with the number of employed plants and the variety of treated ailments, while residency (rural vs. urban) did not. Both residency and education influenced plant procurement methods: rural background and, surprisingly, higher education were linked to a preference for harvesting rather than purchasing plants.

Conclusion Botanical remedies are still commonly used in the treatment of pediatric diseases in Southern Romania, although the variety of taxa seems reduced compared to the past. Further exploration is essential to unlock the maximum benefits of ethnopediatric practices.
Pharmacological targets of SGLT2 inhibitors on IgA nephropathy and membranous nephropathy: a mendelian randomization study
Authors: Lv Xin , Shang Yan , Ning Yong , Yu Weimin , Wang Jian
Published: May 2024

Introduction

Emerging research suggests that sodium-glucose cotransporter 2 (SGLT2) inhibitors may play a pivotal role in the treatment of primary glomerular diseases. This study was aimed to investigate potential pharmacological targets connecting SGLT2 inhibitors with IgA nephropathy (IgAN) and membranous nephropathy (MN).

Methods

A univariate Mendelian randomization (MR) analysis was conducted using publicly available genome-wide association studies (GWAS) datasets. Co-localization analysis was used to identify potential connections between target genes and IgAN and MN. Then, Comparative Toxicogenomics Database (CTD) was employed to predict diseases associated with these target genes and SGLT2 inhibitors (canagliflozin, dapagliflozin, and empagliflozin). Subsequently, phenotypic scan analyses were applied to explore the causal relationships between the predicted diseases and target genes. Finally, we analyzed the immune signaling pathways involving pharmacological target genes using the Kyoto encyclopedia of genes and genomes (KEGG).

Results

The results of MR analysis revealed that eight drug targets were causally linked to the occurrence of IgAN, while 14 drug targets were linked to MN. In the case of IgAN, LCN2 and AGER emerged as co-localized genes related to the pharmacological agent of dapagliflozin and the occurrence of IgAN. LCN2 was identified as a risk factor, while AGER was exhibited a protective role. KEGG analysis revealed that LCN2 is involved in the interleukin (IL)-17 immune signaling pathway, while AGER is associated with the neutrophil extracellular traps (NETs) signaling immune pathway. No positive co-localization results of the target genes were observed between two other SGLT2 inhibitors (canagliflozin and empagliflozin) and the occurrence of IgAN, nor between the three SGLT2 inhibitors and the occurrence of MN.

Conclusion

Our study provided evidence supporting a causal relationship between specific SGLT2 inhibitors and IgAN. Furthermore, we found that dapagliflozin may act on IgAN through the genes LCN2 and AGER.
Species identification of biological ingredients in herbal product, Gurigumu-7, based on DNA barcoding and shotgun metagenomics
Authors: Wei Miaojie , Tian Yu , Zang Erhuan , Tsambaa Battseren , Liu Jinxin , Shi Linchun , Borjigidai Almaz
Published: May 2024

Accurate identification the species composition in mixtures poses a significant challenge, especially in processed mixtures comprising multiple species, such as those found in food and pharmaceuticals. Therefore, we have attempted to utilize shotgun metabarcoding technology to tackle this issue. In this study, the method was initially established using two mock samples of the Mongolian compound preparation Gurigumu-7 (G-7), which was then applied to three pharmaceutical products and 12 hospital-made preparations. A total of 119.72 Gb of raw data sets were obtained through shotgun metagenomic sequencing. By combining ITS2, matK, and rbcL, all the labeled bio-ingredients specified in the G-7 prescription can be detected, although some species may not be detectable in all samples. The prevalent substitution of Akebia quinata can be found in all the pharmaceutical and hospital samples, except for YN02 and YN12. The toxic alternative to Akebia quinata, Aristolochia manshuriensis, was exclusively identified in the YN02 sample. To further confirm this result, we validated it in YN02 using HPLC and real-time PCR with TaqMan probes. The results showed that aristolochic acid A (AAA) was detected in YN02 using HPLC, and the ITS2 sequence of Aristolochia manshuriensis has been validated in YN02 through qPCR and the use of a TaqMan probe. This study confirms that shotgun metabarcoding can effectively identify the biological components in Mongolian medicine compound preparation G-7. It also demonstrates the method’s potential to be utilized as a general identification technique for mixtures containing a variety of plants.
TriticeaeSSRdb: a comprehensive database of simple sequence repeats in Triticeae
Authors: Li Tingting , Cai Shaoshuai , Cai Zhibo , Fu Yi , Liu Wenqiang , Zhu Xiangdong , Lai Chongde , Cui Licao , Pan Wenqiu , Li Yihan
Published: May 2024

Microsatellites, known as simple sequence repeats (SSRs), are short tandem repeats of 1 to 6 nucleotide motifs found in all genomes, particularly eukaryotes. They are widely used as co-dominant markers in genetic analyses and molecular breeding. Triticeae, a tribe of grasses, includes major cereal crops such as bread wheat, barley, and rye, as well as abundant forage and lawn grasses, playing a crucial role in global food production and agriculture. To enhance genetic work and expedite the improvement of Triticeae crops, we have developed TriticeaeSSRdb, an integrated and user-friendly database. It contains 3,891,705 SSRs from 21 species and offers browsing options based on genomic regions, chromosomes, motif types, and repeat motif sequences. Advanced search functions allow personalized searches based on chromosome location and length of SSR. Users can also explore the genes associated with SSRs, design customized primer pairs for PCR validation, and utilize practical tools for whole-genome browsing, sequence alignment, and in silico SSR prediction from local sequences. We continually update TriticeaeSSRdb with additional species and practical utilities. We anticipate that this database will greatly facilitate trait genetic analyses and enhance molecular breeding strategies for Triticeae crops. Researchers can freely access the database at http://triticeaessrdb.com/.
Assessing the Indonesian government's compliance with the public information disclosure law in the context of COVID-19 data transparency
Authors: Khadafi Rizal , Nurmandi Achmad , Hasibuan Effiati Juliana , Harahap Muhammad Said , Saputra Agung , Mahardika Ananda , Izharsyah Jehan Ridho
Published: May 2024

The matter of transparency regarding information and data emerges as a pivotal concern in the context of mitigating the COVID-19 epidemic in Indonesia. The regulation of public information transparency in Indonesia is stipulated in Law Number 14 of 2008, which ensures the disclosure of public information. The objective of this study is to conduct a comprehensive examination of the extent to which the Government of Indonesia adheres to the principles outlined in its legislation with regard to the implementation of transparency throughout the pandemic. The approach employed to assess the degree of adherence is normative-empirical analysis. The findings of this research analysis indicate a significant lack of transparency in the public dissemination of COVID-19 information and data in Indonesia. This lack of transparency is inconsistent with the provisions outlined in Law No. 14 of 2008, which governs the publication of public information in the country. The act of downplaying COVID-19 through the dissemination of information, along with the government's decision to withhold comprehensive data, and the prevailing skepticism toward scientific research might be characterized as efforts to impede citizens' access to precise knowledge.
Adverse childhood experiences and dental anxiety among Chinese adults in Hong Kong: a cross-sectional study
Authors: Wong Natalie Sui Miu , Yeung Andy Wai Kan , McGrath Colman Patrick , Leung Yiu Yan
Published: May 2024

Introduction

This study aimed to investigate the relationship between adverse childhood experiences (ACEs) and dental anxiety among Chinese adults in Hong Kong.

Methods

A cross-sectional survey was conducted at a university in Hong Kong. The recruiting period and data collection started in January 2023 and ended in June 2023. Participants completed an online questionnaire that assessed ACEs (using the Adverse Childhood Experiences International Questionnaire – ACE-IQ) and dental anxiety (using the Modified Dental Anxiety Scale – MDAS and Dental Fear Survey – DFS). The study examined the impacts of both cumulative (i.e., total number) and independent ACE components on dental anxiety. To explore the relationships between cumulative ACEs, individual ACE components and dental anxiety (MDAS and DFS score), Pearson correlations, linear regression and logistic regression were conducted.

Results

Significant associations were observed between ACEs and dental anxiety among 171 subjects. Cumulative ACEs were positively correlated with MDAS scores (r = 0.169, p = 0.027) and DFS scores (r = 0.253, p < 0.001). The odds of an individual having high dental anxiety increased by 26–43% for every additional increase in the number of ACEs. Individual types of ACEs, such as emotional and physical neglect, sexual abuse, and household substance abuse, significantly influenced the likelihood of having high dental anxiety.

Discussion

The results showed a positive association between ACEs and dental anxiety, highlighting the impact of ACEs on dental anxiety. Dental practitioners should consider inquiring about a patient’s ACE history to develop personalized treatment plans.
Perceived controllability of group membership does not moderate individuating information effects in implicit person perception
Authors: Rubinstein Rachel S. , Jussim Lee , Mangracina Brandon , Shaw K. Mackenzie , Yanovsky Sonia , Bennett Samuel
Published: May 2024

Although the effects of counterstereotypic individuating information (i.e., information specific to individual members of stereotyped groups that disconfirms the group stereotype) on biases in explicit person perception are well-established, research shows mixed effects of such information on implicit person perception. The present research tested the overarching hypothesis that, when social group membership is perceived to be under an individual's control, diagnostic individuating information would have lesser effects on implicit person perception than it would when social group membership is perceived not to be under an individual's control. This hypothesis was tested in the domain of implicit attitudinal and stereotype-relevant judgments of individuals who belonged to existing social groups and individuals who belonged to novel social groups. We found that individuating information consistently shifted scores on implicit measures among targets belonging to existing social groups, but not in a theoretically predicted direction among targets belonging to novel social groups. Controllability of group membership did not moderate such effects. Results of implicit and explicit measures were mostly consistent when targets belonged to existing social groups, but mostly inconsistent when targets belonged to novel social groups.
Association of 24-hour movement guideline adherence, mental health and quality of life in young adults: the role of e-Health literacy
Authors: Lin Lixin , Liang Wei , Wang Runbin , Rhodes Ryan E. , Liu Huaxuan
Published: May 2024

Background

The spread of Covid-19 and resultant infection prevention strategies have disturbed the life routine of Chinese young adults, led to reduced physical activity (PA), prolonged screen time (ST) and inadequate sleep duration (SP), and made immense influence on their mental health (MH) and quality of life (QoL). E-Health literacy (EHL) can enable citizens to use available online information to respond to the highly complex information environment and make appropriate health decisions.

Objective

This study aims to examine associations between adhering to 24-h movement (24HM) guidelines and MH and QoL among young adults, as well as to identify any mediating or moderating role of EHL in these associations.

Methods

1742 young adults (20.03 ± 1.54 years old, 68.6% females) from north and south China completed self-report measures of 24HM (PA, ST and SP), health indicators (MH and QoL), EHL and demographic information through an online survey between 4 Apr and 16 Jun 2022. Generalized linear mixed models were applied for data analysis.

Results

Results showed that adhering to PA, ST and SP guidelines were all positively connected with QoL while MH was associated with adhering ST or SP guidelines. Adhering to more of 24HM guidelines was linked to better MH and QoL. EHL significantly mediated the association of guideline adherence and QoL and moderate that of guideline adherence and MH.

Conclusion

This is the first study to investigate the role of EHL on the associations between 24HM and MH as well as QoL during the Covid-19. The findings may contribute to further empirical research or intervention that aims to promote MH or QoL among young adults more effectively or provide valuable references for developing relevant strategies or policy of health promotion or public health events in China.
Distinct time trends in colorectal cancer incidence in countries with SDI levels from 1990 to 2019: an age–period–cohort analysis for the Global Burden of Disease 2019 study
Authors: Zhang Yan , Zhang Xun-Bing , Ding Yu-Wei , Kong Yang , Zhu Xiao-Feng , Li Pu-Heng , Tian Yang , Zhang Qing-Wei
Published: May 2024

Introduction

The burden of colorectal cancer (CRC) plays a pivotal role in the global cancer epidemic. Our study reported the incidence trends in CRC and the associated effects of age, period, and birth cohort in 204 countries and territories over the past 30 years.

Methods

The incidence data of CRC were extracted from the Global Burden of Disease Study (GBD) 2019. We performed the age–period–cohort (APC) model to estimate the overall annual percentage change (net drift) in the incidence rate, the annual percentage change by age group (local drift), and the relative risk (period and cohort effects) of the period and cohort in CRC during 1990–2019. This approach allows examining and distinguishing age, period, and cohort effects in incidence and potentially distinguishing colorectal cancer gaps in prevention and screening.

Results

In 2019, the incidence of CRC was 2.17 (95% UI 2.00–2.34) million, of which China, the United States of America, and Japan had the highest incidence population, accounting for 45.9% of the global population. The age–standardized incidence rate (ASIR) was 26.7 (95% UI 28.9–24.6) per 100,000 people, of which 30 countries had an incidence rate greater than 40.0 per 100,000 people. From 1990 to 2019, the middle SDI region had the largest increase in incidence rate, with a net drift of 2.33% (95% CI 2.2–2.46%, p < 0.001). Globally, the incidence population was concentrated in the age group of 50–69 years, and the age group of 30–34 years had the largest increase in incidence rate (local drift 1.19% (95% CI 1.01–1.37%)). At the same time, the sex and age distributions of CRC incidence had significant heterogeneity across regions and countries. In the past 30 years, the incidence rate in 31 countries has been well controlled (net drift <0), and most of them were concentrated in high–and high–middle–SDI regions, such as Australia, Czechia, and Belgium, and the relative risk of incidence generally improved over time and consecutive young birth cohorts. CRC incidence showed an unfavorable trend (net drift ≥1%) in 89 countries, of which 27 countries were more significant (net drift >2%), mostly concentrated in the middle SDI region, such as China, Mexico, and Brazil, and the risk of period and birth cohort was unfavorable.

Conclusion

Globally, the incidence of CRC has shown an overall upward trend over the past 30 years, with the exception of some countries with higher SDI values. Significant age–period–cohort differences were observed in the risk of incidence in CRC worldwide. Effective prevention and control policies need to take into account the age–period–cohort effect characteristics of different regions.
Clinical predictive value of the CRP-albumin-lymphocyte index for prognosis of critically ill patients with sepsis in intensive care unit: a retrospective single-center observational study
Authors: Zhang Jinhui , Zhao Qun , Liu Shuo , Yuan Nana , Hu Zhenkui
Published: May 2024

Background

Sepsis is a complex syndrome characterized by physiological, pathological, and biochemical abnormalities caused by infection. Its development is influenced by factors such as inflammation, nutrition, and immune status. Therefore, we combined C-reactive protein (CRP), albumin, and lymphocyte, which could reflect above status, to be the CRP-albumin-lymphocyte (CALLY) index, and investigated its association with clinical prognosis of critically ill patients with sepsis.

Methods

This retrospective observational study enrolled critically ill patients with sepsis who had an initial CRP, albumin, and lymphocyte data on the first day of ICU admission. All data were obtained from the Affiliated Hospital of Jiangsu University. The patients were divided into quartiles (Q1–Q4) based on their CALLY index. The outcomes included 30-/60-day mortality and acute kidney injury (AKI) occurrence. The association between the CALLY index and these clinical outcomes in critically ill patients with sepsis was evaluated using Cox proportional hazards and logistic regression analysis.

Results

A total of 1,123 patients (63.0% male) were included in the study. The 30-day and 60-day mortality rates were found to be 28.1 and 33.4%, respectively, while the incidence of AKI was 45.6%. Kaplan–Meier analysis revealed a significant association between higher CALLY index and lower risk of 30-day and 60-day mortality (log-rank p < 0.001). Multivariate Cox proportional hazards analysis indicated that the CALLY index was independently associated with 30-day mortality [HR (95%CI): 0.965 (0.935–0.997); p = 0.030] and 60-day mortality [HR (95%CI): 0.969 (0.941–0.997); p = 0.032]. Additionally, the multivariate logistic regression model showed that the CALLY index served as an independent risk predictor for AKI occurrence [OR (95%CI): 0.982 (0.962–0.998); p = 0.033].

Conclusion

The findings of this study indicated a significant association between the CALLY index and both 30-day and 60-day mortality, as well as the occurrence of AKI, in critically ill patients with sepsis. These findings suggested that the CALLY index may be a valuable tool in identifying sepsis patients who were at high risk for unfavorable outcomes.
Impact of a digital employer-based weight loss program on individuals age 65 or older
Authors: Rogers Renee J. , Doherty Michael , Jones David , Jakicic John M. , Church Timothy S.
Published: May 2024

Introduction

Older adults are not protected from obesity, which has been linked to frailty, cognitive impairment, and other aging-related factors. Intensive lifestyle interventions have been shown to be effective for weight loss in older adults; however, these have typically been highly intensive and less feasible for dissemination. This analysis describes weight loss in a large-scale, commercially available, digital intervention in a subset of older adults.

Methods

Older adults (N = 20,443, males = 6,238; females = 14,205) between 65 and 85 years of age with overweight (43.3%) or obesity (46.7%) participated in an online, self-directed weight loss program. Behavioral-based content was delivered through weekly video lessons within an online platform that included weight and physical activity tracking, an online community, a reference library, and access to coaching support. Self-reported measures taken at the time of entry into the program were used for this analysis (demographics, height, body weight, and health status). Weight was reported across weeks of engagement in the curriculum.

Results

The average weight loss was −3.15 kg (95% CI: [−3.20, −3.11]) at 15.5 weeks. Weight loss was significantly greater in male individuals (−3.79 kg [95% CI: −3.89, −3.71]) versus female individuals (−2.87 kg [95% CI: −2.94, −2.82]) (p < 0.001), with a similar engagement in curriculum weeks. Percent weight loss was statistically significant for all age categories (p < 0.05) and self-reported health conditions (p < 0.05).

Discussion

Short-term weight loss was observed in older adults exposed to a low-touch, self-guided, and digital behavioral-based weight loss program. Weight loss was also observed even in the presence of various chronic health conditions.
Sustainable public food procurement: criteria and actors’ roles and influence
Authors: Molin Elvira , Lingegård Sofia , Martin Michael , Björklund Anna
Published: May 2024

Food production and consumption significantly contribute to climate change. The public sector, which procures large quantities of food, has a crucial role in steering toward more sustainable food systems. More empirical studies involving practitioners are called for to understand the complexity of sustainable public food procurement. This study examines how actors interpret and implement sustainability in food procurement, as well as their influence on this process. A comprehensive analysis of multi-actor collaboration and stakeholder engagement involving interviews with key actors such as procurement officers, wholesalers, chefs, and food producers is presented. Findings highlight a consensus on the need for sustainability measures, such as purchasing local and organic products. The results showcase the potential transformation of power dynamics within the supply chain in response to modifications in procurement standards toward local produce and the overuse of product-specific criteria. However, assuming that local is invariably sustainable carries the risk of falling into the “local trap” when the consequence of procuring local varies and requires further investigation. The influence of system-level factors, including market dynamics and regulatory frameworks, plays a significant role in implementing sustainable procurement, as well as alignment and coordination in the supply chain.
Development of an enhanced recovery after surgery program for pediatric solid tumors
Authors: Mansfield Sara A. , Kotagal Meera , Hartman Stephen , Murphy Andrew J. , Davidoff Andrew M. , Anghelescu Doralina L. , Mecoli Marc , Cost Nicholas , Hogan Brady , Rove Kyle O.
Published: May 2024

Introduction

Enhanced recovery after surgery (ERAS) is an evidence-based, multi-modal approach to decrease surgical stress, expedite recovery, and improve postoperative outcomes. ERAS is increasingly being utilized in pediatric surgery. Its applicability to pediatric patients undergoing abdominal tumor resections remains unknown.

Methods and Analysis

A group of key stakeholders adopted ERAS principles and developed a protocol suitable for the variable complexity of pediatric abdominal solid tumor resections. A multi-center, prospective, propensity-matched case control study was then developed to evaluate the feasibility of the protocol. A pilot-phase was utilized prior to enrollment of all patients older than one month of age undergoing any abdominal, retroperitoneal, or pelvic tumor resections. The primary outcome was 90-day complications per patient. Additional secondary outcomes included: ERAS protocol adherence, length of stay, time to administration of adjuvant chemotherapy, readmissions, reoperations, emergency room visits, pain scores, opioid usage, and differences in Quality of Recovery 9 scores.

Ethics and Dissemination

Institutional review board approval was obtained at all participating centers. Informed consent was obtained from each participating patient. The results of this study will be presented at pertinent society meetings and published in peer-reviewed journals. We expect the results will inform peri-operative care for pediatric surgical oncology patients and provide guidance on initiation of ERAS programs. We anticipate this study will take four years to meet accrual targets and complete follow-up.

Trial Registration Number

NCT04344899.
Effects of clove oil concentrations on blood chemistry and stress-related gene expression in Siamese fighting fish (Betta splendens) during transportation
Authors: Sintuprom Chanoknan , Nuchchanart Wirawan , Dokkaew Sahabhop , Aranyakanont Chak , Ploypan Raveewan , Shinn Andrew P. , Wongwaradechkul Ratchakorn , Dinh-Hung Nguyen , Dong Ha Thanh , Chatchaiphan Satid
Published: May 2024

Siamese fishing fish (Betta splendens) or betta are usually subjected to a special method of transportation for global trade, where they are individually conveyed in plastic bags containing just enough water to cover their bodies. This study aimed to investigate the effects of transportation on their stress response by measuring hematological values, stress hormone levels, glucose levels, and stress-related gene expression. Betta fish (average body weight 1.91 ± 0.42 g; n = 30) were exposed to simulated transport in a water volume of 40 mL for 12, 24, and 48 h. Baseline levels (pre-transport) were measured prior to the experiment. The control group was transported using water without adding clove oil. Two treatment groups were transported using water with the addition of 1 and 3 mg/L concentrations of clove oil, respectively. The results revealed that transportation can be a factor that affects water quality. The pH and dissolved oxygen levels were significantly lower than baseline, while nitrite and total ammonia concentrations significantly increased. Correlating to the stress responses, significantly increasing total red blood cell counts, plasma cortisol levels, and up-regulating the expression of stress-related genes, including HSP70, GR, MR, and HIF-1α. The addition of 1 mg/L clove oil was found to reduce stress during the transport simulation, as evidenced by a reduction in these stress parameters. Conversely, increasing the concentration of clove oil to 3 mg/L significantly increased plasma cortisol after 12 h of simulated transport, and up-regulated GR, MR, and HIF-1α expression. This study revealed that the transport process can stimulates stress in betta fish but adding a concentration of 1 mg/L clove oil to the transport water could mitigate this stress response and promote animal welfare during their transportation.
Establishment and characterization of six canine hepatocellular carcinoma cell lines
Authors: Lee Ja Young , Bae Kieun , Kim Jung-Hyun , Han Hyun-Jung , Yoon Hun-Young , Yoon Kyong-Ah
Published: May 2024

Background

Hepatocellular carcinoma (HCC) is the most common malignant liver tumor in dogs. Although surgical resection is a major treatment option for canine HCC, there are no distinct strategies for unresectable tumor subtypes or adjuvant chemotherapy for tumors with positive margins. We aimed to establish and characterize novel HCC cell lines from canine patients.

Methods

The cellular morphology, general growth features and tumorigenicity of the established cell lines were evaluated. We also examined the sensitivity of the cell lines to multi-target tyrosine kinase inhibitors (TKIs).

Results

We established novel canine HCC cell lines from hepatic tumors and an additional kidney tumor of six canine patients. All cell lines showed colony forming and migratory ability. KU-cHCC-001 and KU-cHCC-001-Kidney, two cell lines exhibiting high epithelial–mesenchymal transition characteristics, showed tumorigenicity in xenografted mice. Toceranib, a veterinary TKI that targets vascular endothelial growth factor (VEGFR)/platelet-derived growth factor receptor (PDGFR)/c-kit, effectively inhibited the mitogen-activated protein kinase pathway and induced apoptosis. The established canine HCC cell lines showed greater sensitivity to toceranib than to sorafenib, a first-line treatment for human HCC targeting RAF/VEGFR/PDGFR. Sorafenib showed improved anti-tumor effects when co-treated with SCH772984, an extracellular signal-regulated kinase inhibitor.

Conclusion

Our study suggests new therapeutic strategies for canine HCC, and these cell lines are valuable research materials for understanding HCC tumor biology in both humans and dogs.
Development of a TaqMan polymerase chain reaction detection method for the precise identification and quantification of an attenuated Eimeria maxima vaccine strain in poultry
Authors: Jin Haozhan , Cai Haiming , Liao Shenquan , Qi Nanshan , Li Juan , Lv Minna , Lin Xuhui , Hu Junjing , Song Yongle , Zhu Yibin , Chen Xiangjie , Yin Lijun , Zhang Xiaohui , Zhang Jianfei , Zhang Xu , Sun Mingfei
Published: May 2024

Avian coccidiosis, a parasitic disease prevalent in poultry, is caused by Eimeria species and leads to significant economic losses. The use of attenuated live oocyst vaccines has been adopted as an alternative to the use of anticoccidial drugs. However, the accurate detection and differentiation of vaccine strains from virulent ones remain challenging. Therefore, this study presents a novel TaqMan polymerase chain reaction (PCR) detection method that offers enhanced sensitivity, specificity, and reproducibility compared with traditional PCR techniques. Through whole-genome resequencing and bioinformatics analysis, we identified a molecular marker gene, Em_marker6, with a unique 21-base pair deletion specific to the Eimeria maxima attenuated vaccine strain. Optimized primers and probes targeting this marker enabled rapid quantification cycle value achievement and high fluorescence intensity. The standard curve’s slope of −3.540 and correlation coefficient of 0.9971 confirmed precise quantification capabilities. The TaqMan PCR method detected as few as 30 plasmid DNA copies and 50 oocysts per reaction, outperforming traditional PCR techniques by an order of magnitude. No cross-reactivity was observed with other E. maxima wide-type strains or common intestinal pathogens, ensuring the exclusive detection of the E. maxima EMPY vaccine strain. Weekly testing over 3 weeks demonstrated minimal variability, indicating robust consistency in the method’s application. Testing on 61 clinical samples revealed a 57.38% positivity rate for E. maxima species and 13.11% for the vaccine strain. The Em_marker6 gene exhibited genetic stability across multiple generations, confirming the detection method’s robust stability for the attenuated E. maxima vaccine strain. This study significantly advances the field of avian coccidiosis research and control by providing a valuable tool for monitoring vaccine purity and preventing inadvertent infections in vaccinated flocks, aligning with global efforts to curb antibiotic use in animal feed.
Multi-dimensional evaluation of pain response in low day-age calves to two types of dehorning
Authors: Cui Weiguo , Liu Mengyu , Gu Tianyu , Zhao Shuai , Yin Guoan
Published: May 2024

Introduction

Dehorning calves is necessary to minimize injury because intensive raising circumstances make horned cows more aggressive. However, acute pain is commonly perceived by farm animals when undergoing painful practices such as dehorning, affecting their health status and quality of life. By quantifying the magnitude of pain and discomfort associated with dehorning, we aim to contribute to a more humane and sustainable cattle farming industry.

Methods

The objective of this study was to evaluate the behavioral, physiological, and emotional effects of acute dehorning pain in calves using two methods: dehorning cream and dehorning hot-iron.30 Holstein calves aged 4 days were selected for the study. These calves were randomly assigned to two experimental groups based on the method of disbudding: dehorning cream (n = 15) and hot-iron dehorning (n = 15). Before and after dehorning, we evaluated their physiological indicators of infrared eye temperature, concentrations of substance P, IL-6, cortisol, haptoglobin, as well as emotional state, and pain-related behavioral reactions.

Results

Post-dehorning, the duration of lying down decreased significantly in both groups (DI and DC: 0–4 h) after dehorning (p < 0.05). Both groups exhibited increased frequencies of pain-related behaviors such as head shaking (DI: 1–7 h, DC: 1–6 h), ear flicking (DI: 2–7 h, DC: 2–7 h), head scratching (DI: 2–3 h, DC: 1–7 h), and top scuffing (DI: 2 h, DC: 2–7 h) compared to pre-dehorning (p < 0.05). The DC group demonstrated a higher frequency of head-shaking, ear-flicking, head-scratching, and top-rubbing behaviors, along with a longer duration of lying down (0–4 h), compared to the DI group (p < 0.05). Post-dehorning, play behavior reduced significantly in both groups (6–8 h) (p < 0.05), whereas judgment bias and fear levels showed no significant change (p > 0.05). Physiological measures including eye temperature, and blood levels of substance P and IL-6, did not differ significantly between the groups before and after dehorning (p > 0.05). However, 48 h after dehorning, calves in the DC group had significantly higher haptoglobin levels compared to the DI group (p = 0.015). Additionally, salivary cortisol levels in the DC group increased significantly at 3.5 h and 7 h post-dehorning (p = 0.018, p = 0.043).

Discussion

Both hot-iron and cream dehorning induced pain in calves, as evidenced by increased pain-related behaviors, elevated salivary cortisol, and higher haptoglobin levels, alongside reduced positive behaviors. Notably, these effects were more pronounced in the DC group than in the DI group, suggesting that dehorning hot-iron may be a comparatively less stressful dehorning method for young calves. Moreover, the brief duration of pain response and weaker response to dehorning observed in 13-day-age calves in this study suggests that dehorning at younger ages may be more advisable and warrants further research.
Impact of Industry 5.0 Readiness on Sustainable Business Growth of Marine Food Processing SMEs in Thailand
Authors: Madhavan Meena , Sharafuddin Mohammed Ali , Wangtueai Sutee
Published: May 2024

This research aims to develop a conceptual framework and propositions to establish and test the causal relationships between Industry 5.0 readiness (I5.0R), global value chain (GVC) participation, business competitiveness (BC), and sustainable business growth (SBG) of small and medium-sized enterprises (SMEs). This study focuses on Industry 5.0 readiness, evaluated through human-centricity, fairtrade practices, lean management, sustainability practices, and business competitiveness, measured by marketing, resources, production, and finance. Both constructs were developed and tested as higher-order constructs, while GVC participation and sustainable business growth were assessed as lower-order constructs. Data were collected from marine food processing SMEs in Thailand using a purposive sampling technique. This study tested and confirmed the content validity, construct validity, and reliability of both lower and higher-order models. Using partial least squares structural equation modeling (PLS-SEM) with bootstrapping (n = 1000), the results indicated significant positive impacts of Industry 5.0 readiness on GVC participation, Industry 5.0 readiness on business competitiveness, GVC participation on business competitiveness, and business competitiveness on the sustainable business growth of SMEs. Additionally, business competitiveness was found to mediate the relationship between Industry 5.0 readiness and sustainable business growth. These findings contribute to the literature on Industry 5.0, GVCs, and SME business competitiveness, offering practical insights for SMEs and policymakers aiming to enhance sustainable growth through strategic readiness and competitiveness in Industry 5.0 practices. The implications and directions for further research in Industry 5.0 readiness of SMEs are presented.
The Relationship between Geographical Indication Products and Farmers’ Incomes Based on Meta-Analysis
Authors: Li Chunyan , Ban Qi , Ge Lanqing , Qi Liwen , Fan Chenchen
Published: May 2024

Geographical indication (GI) products serve as one of the significant instruments for increasing farmers’ income. While most studies affirmatively indicate that GI products contribute to boosting farmers’ income growth, it is noteworthy that their relationship does not consistently demonstrate a positive correlation. The academic discourse on this issue remains inconclusive. This study employs a meta-analysis method to reanalyze 140 effect sizes from 32 independent research samples across diverse global contexts. The findings reveal that the development of GI products significantly promotes farmer income growth, showing a high positive correlation (r = 0.348, CI = [0.104, 0.540]). Specifically, there exists a high positive correlation between GI products and per capita disposable income (r = 0.389) and a moderate positive correlation between GI products and agricultural product prices (r = 0.255). Further analysis indicates that factors at the sample level, literature level, and methodological level all exert moderating effects on the relationship between GI products and farmers’ income. This study not only provides a scientific response to the debate surrounding the relationship between GI products and farmers’ income but also delves into the underlying mechanisms. It holds significant importance for advancing the rational optimization of agricultural resources and enhancing agricultural competitiveness.
Intelligent Detection of Muskmelon Ripeness in Greenhouse Environment Based on YOLO-RFEW
Authors: Xu Defang , Ren Rui , Zhao Huamin , Zhang Shujuan
Published: May 2024

Accurate detection of muskmelon fruit ripeness is crucial to ensure fruit quality, optimize picking time, and enhance economic benefits. This study proposes an improved lightweight YOLO-RFEW model based on YOLOv8n, aiming to address the challenges of low efficiency in muskmelon fruit ripeness detection and the complexity of deploying a target detection model to a muskmelon picking robot. Firstly, the RFAConv replaces the Conv in the backbone part of YOLOv8n, allowing the network to focus more on regions with significant contributions in feature extraction. Secondly, the feature extraction and fusion capability are enhanced by improving the C2f module into a C2f-FE module based on FasterNet and an Efficient Multi-Scale attention (EMA) mechanism within the lightweight model. Finally, Weighted Intersection over Union (WIoU) is optimized as the loss function to improve target frame prediction capability and enhance target detection accuracy. The experimental results demonstrate that the YOLO-RFEW model achieves high accuracy, with precision, recall, F1 score, and mean Average Precision (mAP) values of 93.16%, 83.22%, 87.91%, and 90.82%, respectively. Moreover, it maintains a lightweight design and high efficiency with a model size of 4.75 MB and an inference time of 1.5 ms. Additionally, in the two types of maturity tests (M-u and M-r), APs of 87.70% and 93.94% are obtained, respectively, by the YOLO-RFEW model. Compared to YOLOv8n, significant improvements in detection accuracy have been achieved while reducing both model size and computational complexity using the proposed approach for muskmelon picking robots’ real-time detection requirements. Furthermore, when compared to lightweight models such as YOLOv3-Tiny, YOLOv4-Tiny, YOLOv5s, YOLOv7-Tiny, YOLOv8s, and YOLOv8n, the YOLO-RFEW model demonstrates superior performance with only 28.55%, 22.42%, 24.50%, 40.56%, 22.12%, and 79.83% of their respective model sizes, respectively, while achieving the highest F1 score and mAP values among these seven models. The feasibility and effectiveness of our improved scheme are verified through comparisons between thermograms generated by YOLOv8n and YOLO-RFEW as well as detection images. In summary, the YOLO-RFEW model not only improves the accuracy rate of muskmelon ripeness detection but also successfully realizes the lightweight and efficient performance, which has important theoretical support and application value in the field of muskmelon picking robot development.
The Impact of Abrupt and Fenceline-Weaning Methods on Cattle Stress Response, Live Weight Gain, and Behaviour
Authors: Mac Sarah E. , Lomax Sabrina , Doughty Amanda K. , Thomson Peter C. , Clark Cameron E. F.
Published: May 2024

The impact of abrupt (AB) and fenceline (FL) weaning methods on cattle stress response, live weight gain, and behaviour were determined across 14 days. Thirty-two cow–calf pairs were fitted with ear tag sensors (to continuously record behaviour) and allocated to two weaning treatments. After separation, FL calves were maintained in a pen adjacent to the FL cow paddock. The AB calves were transported to a pen removing all contact with the cows. After 7 d, FL cows were transported away from all calf pens. Body weights and salivary samples were collected for all animals on experimental days 0, 7, and 14. Fenceline-weaned calves had a greater duration of rest and rumination with reduced high activity across the first 3 days after separation as compared to abruptly weaned calves in line with the greater occurrences of pacing observed for AB calves. Fenceline-separated cows had greater levels of rest across the first 7 days but similar levels of rumination compared to abruptly separated cows. Fenceline-separated cow activity levels tended to be greater and eating levels were similar across the first three days. Body weight (BW) and cortisol concentrations were similar for AB and FL cattle, but FL cows had lower overall weight gain than the abrupt cows likely due to reduced eating time on days four to seven. Together, these results suggest that calves be fenceline-weaned for three days followed by total separation.