bluuebunny/crossref_metadata_2025 huggingface dataset:
Large-Scale Geographic Size Variability of Cyprideis torosa (Ostracoda) and Its Taxonomic and Ecologic Implications
Authors: Wrozyna Claudia , Mischke Steffen , Hoehle Marlene , Gross Martin , Piller Werner E.
Published: July 2022

Body-size variability results from a variety of extrinsic and intrinsic factors (environmental and biological influences) underpinned by phylogeny. In ostracodes it is assumed that body size is predominantly controlled by ecological conditions, but investigations have mostly focused on local or regional study areas. In this study, we investigate the geographical size variability (length, height, and width) of Holocene and Recent valves of the salinity-tolerant ostracode species Cyprideis torosa within a large geographical area (31°–51° latitude, and 12°–96° longitude). It is shown that distant local size clusters of Cyprideis torosa are framed within two large-scale geographical patterns. One pattern describes the separation of two different size classes (i.e., morphotypes) at around ∼42° N. The co-occurrence of both size morphotypes in the same habitats excludes an environmental control on the distribution of the morphotypes but rather could point to the existence of two differentiated lineages. Generally, correlations between valve size and environmental parameters (salinity, geographical positions) strongly depend on the taxonomic resolution. While latitude explains the overall size variability of C. torosa sensu lato (i.e., undifferentiated for morphotypes), salinity-size correlations are restricted to the morphotype scale. Another large-scale pattern represents a continuous increase in valve size of C. torosa with latitude according to the macroecological pattern referred as Bergmann trend. Existing explanations for Bergmann trends insufficiently clarify the size cline of C. torosa which might be because these models are restricted to intraspecific levels. The observed size-latitude relationship of C. torosa may, therefore, result from interspecific divergence (i.e., size ordered spatially may result from interspecific divergence sorting) while environmental influence is of minor importance. Our results imply that geographical body-size patterns of ostracodes are not straightforward and are probably not caused by universal mechanisms. Consideration of phylogenetic relationships of ostracodes is therefore necessary before attempting to identify the role of environmental controls on body size variability.
Crohn’s disease recurrence updates: first surgery vs. surgical relapse patients display different profiles of ileal microbiota and systemic microbial-associated inflammatory factors
Authors: Russo Edda , Cinci Lorenzo , Di Gloria Leandro , Baldi Simone , D’Ambrosio Mario , Nannini Giulia , Bigagli Elisabetta , Curini Lavinia , Pallecchi Marco , Andrea Arcese Donato , Scaringi Stefano , Malentacchi Cecilia , Bartolucci Gianluca , Ramazzotti Matteo , Luceri Cristina , Amedei Amedeo , Giudici Francesco
Published: July 2022

Background and aims

Crohn’s disease (CD) pathogenesis is still unclear. Remodeling in mucosal microbiota and systemic immunoregulation may represent an important component in tissue injury. Here, we aim to characterize the ileal microbiota in both pathological and healthy settings and to evaluate the correlated systemic microbial-associated inflammatory markers comparing first-time surgery and relapse clinical conditions.

Methods

We enrolled 28 CD patients at surgery; we collected inflamed and non-inflamed mucosa tissues and blood samples from each patient. Bacterial wall adherence was observed histologically, while its composition was assessed through amplicon sequencing of the 16S rRNA gene. In addition, we evaluated the systemic microRNA (miRNA) using quantitative real-time PCR amplification and free fatty acids (FFAs) using gas chromatography–mass spectroscopy.

Results

The total number of mucosal adherent microbiota was enriched in healthy compared to inflamed mucosa. In contrast, the phylum Tenericutes, the family Ruminococcaceae, and the genera Mesoplasma and Mycoplasma were significantly enriched in the pathological setting. Significant microbiota differences were observed between the relapse and first surgery patients regarding the families Bacillaceae 2 and Brucellaceae and the genera Escherichia/Shigella, Finegoldia, Antrobacter, Gemmatimonas, Moraxella, Anoxibacillus, and Proteus. At the systemic level, we observed a significant downregulation of circulating miR-155 and miR-223, as well as 2-methyl butyric, isobutyric, and hexanoic (caproic) acids in recurrence compared to the first surgery patients. In addition, the level of hexanoic acid seems to act as a predictor of recurrence risk in CD patients (OR 18; 95% confidence interval 1.24–261.81; p = 0.006).

Conclusions

We describe a dissimilarity of ileal microbiota composition comparing CD and healthy settings, as well as systemic microbial-associated inflammatory factors between first surgery and surgical relapse. We suggest that patterns of microbiota, associated with healthy ileal tissue, could be involved in triggering CD recurrence. Our findings may provide insight into the dynamics of the gut microbiota–immunity axis in CD surgical recurrence, paving the way for new diagnostics and therapeutics aimed not only at reducing inflammation but also at maintaining a general state of eubiosis in healthy tissue.
New vistas unfold: Chicken MHC molecules reveal unexpected ways to present peptides to the immune system
Authors: Halabi Samer , Kaufman Jim
Published: July 2022

The functions of a wide variety of molecules with structures similar to the classical class I and class II molecules encoded by the major histocompatibility complex (MHC) have been studied by biochemical and structural studies over decades, with many aspects for humans and mice now enshrined in textbooks as dogma. However, there is much variation of the MHC and MHC molecules among the other jawed vertebrates, understood in the most detail for the domestic chicken. Among the many unexpected features in chickens is the co-evolution between polymorphic TAP and tapasin genes with a dominantly-expressed class I gene based on a different genomic arrangement compared to typical mammals. Another important discovery was the hierarchy of class I alleles for a suite of properties including size of peptide repertoire, stability and cell surface expression level, which is also found in humans although not as extreme, and which led to the concept of generalists and specialists in response to infectious pathogens. Structural studies of chicken class I molecules have provided molecular explanations for the differences in peptide binding compared to typical mammals. These unexpected phenomena include the stringent binding with three anchor residues and acidic residues at the peptide C-terminus for fastidious alleles, and the remodelling binding sites, relaxed binding of anchor residues in broad hydrophobic pockets and extension at the peptide C-terminus for promiscuous alleles. The first few studies for chicken class II molecules have already uncovered unanticipated structural features, including an allele that binds peptides by a decamer core. It seems likely that the understanding of how MHC molecules bind and present peptides to lymphocytes will broaden considerably with further unexpected discoveries through biochemical and structural studies for chickens and other non-mammalian vertebrates.
Methotrexate Inhibits T Cell Proliferation but Not Inflammatory Cytokine Expression to Modulate Immunity in People Living With HIV
Authors: Freeman Michael L. , Clagett Brian M. , Moisi Daniela , Yeh Eunice , Morris Charles D. , Ryu Angela , Rodriguez Benigno , Stein James H. , Deeks Steven G. , Currier Judith S. , Hsue Priscilla Y. , Anthony Donald D. , Calabrese Leonard H. , Ribaudo Heather J. , Lederman Michael M.
Published: July 2022

Inflammation associated with increased risk of comorbidities persists in people living with HIV (PWH) on combination antiretroviral therapy (ART). A recent placebo-controlled trial of low-dose methotrexate (MTX) in PWH found that numbers of total CD4 and CD8 T cells decreased in the low-dose MTX arm. In this report we analyzed T cell phenotypes and additional plasma inflammatory indices in samples from the trial. We found that cycling (Ki67+) T cells lacking Bcl-2 were reduced by MTX but plasma inflammatory cytokines were largely unaffected. In a series of in vitro experiments to further investigate the mechanisms of MTX activity, we found that MTX did not inhibit effector cytokine production but inhibited T cell proliferation downstream of mTOR activation, mitochondrial function, and cell cycle entry. This inhibitory effect was reversible with folinic acid, suggesting low-dose MTX exerts anti-inflammatory effects in vivo in PWH largely by blocking T cell proliferation via dihydrofolate reductase inhibition, yet daily administration of folic acid did not rescue this effect in trial participants. Our findings identify the main mechanism of action of this widely used anti-inflammatory medicine in PWH and may provide insight into how MTX works in the setting of other inflammatory conditions.
A novel aGAPSS-based nomogram for the prediction of ischemic stroke in patients with antiphospholipid syndrome
Authors: Song Xiaodong , Fan Yangyi , Jia Yuan , Li Gongming , Liu Meige , Xu Yicheng , Zhang Jun , Li Chun
Published: July 2022

Background

Ischemic stroke (IS) is the most common and life-threatening arterial manifestation of antiphospholipid syndrome (APS). It is related to high mortality and severe permanent disability in survivors. Thus, it is essential to identify patients with APS at high risk of IS and adopt individual-level preventive measures. This study was conducted to identify risk factors for IS in patients with APS and to develop a nomogram specifically for IS prediction in these patients by combining the adjusted Global Anti-Phospholipid Syndrome Score (aGAPSS) with additional clinical and laboratory data.

Methods

A total of 478 consecutive patients with APS were enrolled retrospectively. All patients were randomly assigned to the training and validation cohorts. Univariate and multivariate binary logistic analyses were conducted to identify predictors of IS in the training cohort. Then, a nomogram was developed based on these predictors. The predictive performance of the nomogram for the training and validation cohorts was evaluated by determining areas under the receiver operating characteristic curve (AUROC) and creating calibration plots. A decision curve analysis (DCA) was conducted to compare the potential net benefits of the nomogram with those of the aGAPSS.

Results

During a mean follow-up period of 2.7 years, 26.9% (129/478) of the patients were diagnosed with IS. Binary logistic regression analysis revealed that five risk factors were independent clinical predictors of IS: age (P < 0.001), diabetes (P = 0.030), hyperuricemia (P < 0.001), the platelet count (P = 0.001), and the aGAPSS (P = 0.001). These predictors were incorporated into the nomogram, named the aGAPSS-IS. The nomogram showed satisfactory performance in the training [AUROC = 0.853 (95% CI, 0.802–0.896] and validation [AUROC = 0.793 (95% CI, 0.737–0.843)] cohorts. Calibration curves showed good concordance between observed and nomogram-predicted probability in the training and validation cohorts. The DCA confirmed that the aGAPSS-IS provided more net benefits than the aGAPSS in both cohorts.

Conclusion

Age, diabetes, hyperuricemia, the platelet count, and the aGAPSS were risk factors for IS in patients with APS. The aGAPSS-IS may be a good tool for IS risk stratification for patients with APS based on routinely available data.
Development and validation of a cancer-associated fibroblast-derived lncRNA signature for predicting clinical outcomes in colorectal cancer
Authors: Pan Hongda , Pan Jingxin , Wu Jianghong
Published: July 2022

Cancer-associated fibroblasts (CAFs) are actively involved in cancer progression through generating extracellular matrix and orchestrating the crosstalk within the tumor microenvironment (TME). This study aimed to develop and validate a CAF-derived lncRNA (long non-coding RNA) (CAFDL) signature for predicting clinical outcomes in colorectal cancer (CRC). Clinical data and transcriptomic profiles of 2,320 patients with CRC from The Cancer Genome Atlas (TCGA)-COAD and TCGA-READ datasets and 16 Gene Expression Omnibus datasets were included in this study. CAFDLs were identified using weighted gene co-expression network analysis. The CAFDL signature was constructed using the least absolute shrinkage and selection operator analysis in the TCGA-CRC training set. Multiple CRC cohorts and pan-cancer cohorts were used to validated the CAFDL signature. Patients with high CAFDL scores had significantly worse overall survival and disease-free survival than patients with low CAFDL scores in all CRC cohorts. In addition, non-responders to fluorouracil, leucovorin, and oxaliplatin (FOLFOX)/fluorouracil, leucovorin, and irinotecan (FOLFIRI) chemotherapy, chemoradiotherapy, bevacizumab, and immune checkpoint inhibitors had significantly higher CAFDL scores compared with responders. Pan-cancer analysis showed that CAFDL had prognostic predictive power in multiple cancers such as lung adenocarcinoma, breast invasive carcinoma, stomach adenocarcinoma, and thyroid carcinoma. The CAFDL signature was positively correlated with transforming growth factor-beta (TGF-β) signaling, epithelial–mesenchymal transition, and angiogenesis pathways but negatively correlated with the expression of immune checkpoints such as PDCD1, CD274, and CTLA4. The CAFDL signature reflects CAF properties from a lncRNA perspective and effectively predicts clinical outcomes in CRC and across pan-cancer. The CAFDL signature can serve as a useful tool for risk stratification and provide new insights into the underlying mechanisms of CAFs in cancer immunity.
Impaired sweating in patients with cholinergic urticaria is linked to low expression of acetylcholine receptor CHRM3 and acetylcholine esterase in sweat glands
Authors: Wang Yiyu , Scheffel Jörg , Vera Carolina Ayala , Liu Wei , Günzel Dorothee , Terhorst-Molawi Dorothea , Maurer Marcus , Altrichter Sabine
Published: July 2022

Background

Cholinergic urticaria (CholU), a frequent form of chronic inducible urticaria, is characterized by itchy wheals and angioedema in response to sweating. As of now, the rate and pathophysiological relevance of impaired sweating in patients with CholU are ill-defined.

Aim

To assess in CholU patients the rate and extent of impaired sweating and its links to clinical and pathophysiological features of CholU.

Patients and methods

We assessed sweating in patients with CholU (n = 13) subjected to pulse-controlled ergometry (PCE) provocation testing. Pre- and post-PCE biopsies of lesional (L) and non-lesional (NL) skin were analyzed for the expression of acetylcholine receptor M3 (CHRM3) and acetylcholine esterase (ACh-E) by quantitative histomorphometry and compared to those of healthy control subjects (HCs). CholU patients were assessed for disease duration and severity as well as other clinical features.

Results

Of the 13 patients with CholU, 10 showed reduced sweating in response to PCE provocation, and 3 had severely reduced sweating. Reduced sweating was linked to long disease duration and high disease severity. CholU patients with impaired sweating responses showed reduced sweat gland epithelial expression of CHRM3 and ACh-E.

Conclusion

Reduced sweating is common in CholU patients, especially in those with long-standing and severe disease, and it can be severe. Reduced expression of CHRM3 and ACh-E may be the cause or consequence of CholU in patients with impaired sweating, and this should be explored by further studies.
Alternative RNA splicing in cancer: what about adult T-cell leukemia?
Authors: Tram Julie , Mesnard Jean-Michel , Peloponese Jean-Marie
Published: August 2022

Eukaryotic cells employ a broad range of mechanisms to regulate gene expression. Among others, mRNA alternative splicing is a key process. It consists of introns removal from an immature mRNA (pre-mRNA) via a transesterification reaction to create a mature mRNA molecule. Large-scale genomic studies have shown that in the human genome, almost 95% of protein-encoding genes go through alternative splicing and produce transcripts with different exons combinations (and sometimes retained introns), thus increasing the proteome diversity. Considering the importance of RNA regulation in cellular proliferation, survival, and differentiation, alterations in the alternative splicing pathway have been linked to several human cancers, including adult T-cell leukemia/lymphoma (ATL). ATL is an aggressive and fatal malignancy caused by the Human T-cell leukemia virus type 1 (HTLV-1). HTLV-1 genome encodes for two oncoproteins: Tax and HBZ, both playing significant roles in the transformation of infected cells and ATL onset. Here, we review current knowledge on alternative splicing and its link to cancers and reflect on how dysregulation of this pathway could participate in HTLV-1-induced cellular transformation and adult T-cell leukemia/lymphoma development.
Toward Regional Marine Ecological Forecasting Using Global Climate Model Predictions From Subseasonal to Decadal Timescales: Bottlenecks and Recommendations
Authors: Minobe Shoshiro , Capotondi Antonietta , Jacox Michael G. , Nonaka Masami , Rykaczewski Ryan R.
Published: August 2022

This perspective paper discusses how the research community can promote enhancement of marine ecosystem forecasts using physical ocean conditions predicted by global climate models (GCMs). We review the major climate prediction projects and outline new research opportunities to achieve skillful marine biological forecasts. Physical ocean conditions are operationally predicted for subseasonal to seasonal timescales, and multi-year predictions have been enhanced recently. However, forecasting applications are currently limited by the availability of oceanic data; most subseasonal-to-seasonal prediction projects make only sea-surface temperature (SST) publicly available, though other variables useful for biological forecasts are also calculated in GCMs. To resolve the bottleneck of data availability, we recommend that climate prediction centers increase the range of ocean data available to the public, perhaps starting with an expanded suite of 2-dimensional variables, whose storage requirements are much smaller than 3-dimensional variables. Allowing forecast output to be downloaded for a selected region, rather than the whole globe, would also facilitate uptake. We highlight new research opportunities in both physical forecasting (e.g., new approaches to dynamical and statistical downscaling) and biological forecasting (e.g., conducting biological reforecasting experiments) and offer lessons learned to help guide their development. In order to accelerate this research area, we also suggest establishing case studies (i.e., particular climate and biological events as prediction targets) to improve coordination. Advancing our capacity for marine biological forecasting is crucial for the success of the UN Decade of Ocean Science, for which one of seven desired outcomes is “A Predicted Ocean”.
Physiological characterization of Typhlatya cave shrimps: linking habitat with aerobic metabolism
Authors: Chávez-Solís Efraín M. , Díaz Fernando , Paschke Kurt , Re Denise , Rosas Carlos , Rodríguez-Fuentes Gabriela , Tremblay Nelly , Mascaró Maite
Published: July 2022

The anchialine environment is characterized by a vertical stratification of water masses with different salinities. Cave shrimps of the genus Typhlatya are widespread inhabitants of the aquifer in fresh, brackish, and marine groundwater. Here we describe physiological aspects of three of the most abundant and widespread Typhlatya species that thrive in the fresh and marine groundwater habitats of the anchialine ecosystem of the Yucatan Peninsula. The aerobic scope (AS) of Typhlatya mitchelli, Typhlatya pearsei and Typhlatya dzilamensis was estimated through induced physical activity, whilst monitoring protein carbonylation and lipid peroxidation (as indicators of cellular damage), lactate accumulation (as an indicator of anaerobic metabolism) and the antioxidant system response. The critical thermal limits (CTL) of all three species as an additional measure of physiological plasticity were also determined. Our results showed that metabolic rates, AS and CTL were similar amongst the two species that inhabit fresh groundwater habitats, and differed markedly from T. dzilamensis, a species typically found in marine groundwater. The antioxidant system response in all three Typhlatya species accompanied the levels of aerobic metabolism following physical activity. However, the large amount of GSH observed in T. dzilamensis may be indicative of an adaptive trait to a more heterogeneous environment. The differences observed among Typhlatya species reflect different physiological adaptations that correspond to the environmental heterogeneity of their natural habitats. Our results suggest that the marine groundwater species, T. dzilamensis, could be better prepared to respond to a naturally more heterogeneous environment, in contrast to Typhlatya mitchelli and Typhlatya pearsei which rarely face environmental clines in the fresh groundwater habitat. Our findings contribute to a better understanding of the consequences of environmental change on ecologically important species that are restricted to live in the aquifer.
Assessing the potential of the unexploited Atlantic purple sea urchin, Arbacia punctulata, for the edible market
Authors: Suckling Coleen C. , Zavell Max D. , Byczynski Anna L. , Takeda Brian T.
Published: August 2022

The global demand for sea urchin as seafood is currently unmet. Despite exploitation of > 40 species across the world, there is a need to identify other candidate species, especially in regions where diversification in production is sought where species are considered native. The Eastern US presents an opportunity to determine the marketability of the currently unexploited Arbacia punctulata which is naturally distributed from Massachusetts and southwards into the Gulf of Mexico. To determine whether A. punctulata had market potential, it was fed one of the following diets to determine whether the gonad tissue (uni) could be manipulated to increase gonad mass and improve gonad color for the market: dried Ulva lactuca, Salmon pellets (Skretting), Tilapia pellets (Ziegler) or an Urchinomics diet designed for sea urchins either fed for 8 weeks or 12 weeks. All of the pelleted feeds (Salmon, Tilapia and Urchinomics) increased gonad mass and altered the color. The colors of the uni were generally darker than the colors that the market would typically prefer but some individuals did exhibit colors which have been classed as acceptable to the European market. This work highlights that further research is worthwhile to assess the market potential of A. punctulata.
Evaluating the quality of environmental baselines for deep seabed mining
Authors: Christiansen Sabine , Bräger Stefan , Jaeckel Aline
Published: August 2022

Generating environmental baseline knowledge is a prerequisite for evaluating and predicting the effects of future deep seabed mining on the seafloor and in the water column. Without baselines, we lack the information against which to assess impacts and therefore cannot decide whether or not they pose an acceptable risk to the marine environment. At present, the International Seabed Authority (ISA), which is the international regulator for seabed mining, requires contractors engaged in mineral exploration to establish geological and environmental baselines for their respective contract areas. However, there are no criteria for evaluating what a robust baseline entails. This paper seeks to address this gap by not only analyzing the role and importance of baselines for environmental management but also suggesting criteria for evaluating the quality of baselines. Such criteria (which we present in tabular format) should include at least a minimum amount of technical information, based on best available scientific information and process, in standardized format to enable comparison between contractors and regional synthesis. These criteria should also allow baselines to be used for before-after comparisons through the choice of appropriate zones for comparison of impacts, and to prepare and test a suite of monitoring indicators and their metrics. Baseline studies should identify uncertainties, vulnerable species and habitats, and include transparent reporting as well as exchange with independent scientists and other stakeholders. The quality criteria suggested in this paper build on the ISA’s existing Mining Code and seek to support the development of a more standardized catalogue of requirements for environmental baselines. This will allow states, mining operators, the ISA, and the public to gain a better understanding of the environmental impacts of seabed mining and available mitigation measures.
Optimizing cryopreservation of sea cucumber (Apostichopus japonicus) sperm using a programmable freezer and computer-assisted sperm analysis
Authors: Xu Shuai , Liu Shilin , Sun Jingchun , Zhang Libin , Lin Chenggang , Sun Lina , Xing Lili , Jiang Chunxi , Yang Hongsheng
Published: August 2022

The sea cucumber Apostichopus japonicus has high nutritional, medicinal, and economic value. However, factors such as overexploitation, climate change, and environmental pollution have resulted in serious germplasm degradation in both farmed and wild A. japonicus, and it has been listed as endangered on the IUCN (International Union for Conservation of Nature) Red List of Threatened Species. Cryopreservation is an important method to protect germplasm resources and solve the problem of germplasm degradation. Using a programmable freezer and computer-aided sperm analysis, we comprehensively studied and screened the factors that affect the post-thaw motility of A. japonicus sperm during cryopreservation. Based on our results, we propose the following optimal cryopreservation procedure for A. japonicus sperm: cryo-diluent composition of 12.5% dimethyl sulfoxide and 0.1 mol/L glucose, with filter-sterilized (the filter mesh size: 0.45μm) natural seawater (NSW) as the extender; 1:5 mixing ratio of sperm and cryo-diluent; cooling rate and thawing temperatures of 10°C/min and 20°C, respectively. The post-thaw motility of sperm treated using the optimal procedure was > 65%, the fertilization rate (in the blastocyst stage) was nearly 80%, and the hatching rate (in the early auricularia larva stage) was > 65%. Additionally, frozen sperm that had been cryopreserved for 1 year retained a considerable post-thaw motility and fertilization rate compared to recently cryopreserved sperm. We detected obvious differences in sperm freezability among individual A. japonicus, and cryopreservation caused some damage to the sperm structure. In conclusion, our optimized procedure make large-scale cryopreservation of A. japonicus sperm possible, and our results provide valuable information that could be applied to research and conservation of A. japonicus.
Research on target detection of Engraulis japonicus purse seine based on improved model of YOLOv5
Authors: Zhang Jiaze , Wang Shuxian , Zhang Shengmao , Tang Fenghua , Fan Wei , Yang Shenglong , Sun Yongwen , Liu Yang , Zhu Wenbin , Zhang Shan , He Ruilin
Published: July 2022

The refined monitoring and identification of fishing operations by fishing vessels is of great significance and value to fishing vessels. In order to solve the problem of inaccurate statistics of current Engraulis japonicus fishing quota and classification, this paper proposes an improved identification algorithm based on YOLOv5. This method introduces the SENet attention mechanism into the YOLOv5 backbone network structure, integrates the target information in different periods of fishing operations, reduces the interference of complex backgrounds, improves the precision of model detection, and ensures real-time detection efficiency. The artificially shot Engraulis japonicus videos are used as the dataset of the study, and the video is converted into a picture format to realize pre-labeling and processing. The 5550 images are divided into the training set, validation set, and test set according to 8:1:1. In order to verify the validity of the data, a set of in the control experiment, the YOLOv5 backbone network was replaced by MobileNetV2 and the SENet attention mechanism was introduced, and four models were implemented for comparison. The experimental results show that the research algorithm can obtain a mean average precision (mAP) of 99.3%, a precision of 98.9%, and a recall of 98.7%, which are improved by 1.4%, 1.7% and 2.5% respectively compared with the original model. The experimental results match the expectations. According to the statistics of some categories, the Kalman filter and the Hungarian matching method are used to count the main categories of fishing baskets, and an accuracy of 96.5% can be obtained. The threshold method for fishing nets and processing vessels can obtain 85.8% and 75% accuracy. These results shows that this target detection research can provide new ideas for job identification of Engraulis japonicus and provide auxiliary means for job statistics.
Case report: Bladder preserving after maximal transurethral resection of the bladder tumor combined with chemotherapy and immunotherapy in recurrent muscle-invasive bladder cancer patients: A report of two cases
Authors: Mao Jiaquan , Yang Chunguang , Xin Sheng , Cui Kai , Liu Zheng , Wang Tao , Hu Zhiquan , Wang Shaogang , Liu Jihong , Song Xiaodong , Song Wen
Published: August 2022

Background

Cisplatin-based neoadjuvant chemotherapy combined with radical cystectomy (RC) plus pelvic lymph node dissection (PLND) is the preferred treatment option for muscle-invasive bladder cancer (MIBC). However, some patients are unable to tolerate RC or may have postoperative complications after RC. And most patients have a strong desire for bladder-preserving treatment. There are no reports on the efficacy of maximal transurethral resection of the bladder tumor (TURBT) in combination with chemotherapy plus tislelizumab for bladder-preserving in recurrent MIBC patients.

Case presentation

We report two cases diagnosed with recurrent MIBC who achieved pathological complete response (pCR) and bladder-preserving after maximal TURBT combined with chemotherapy plus tislelizumab.

Conclusion

Postoperative immunotherapy should be considered for all patients with recurrent MIBC who are eligible for immunotherapy. In addition, high programmed death ligand-1 (PD-L1) expression, high tumor mutation burden (TMB), and TP53 mutation level can be combined to predict tislelizumab efficacy.
Protease-activated receptor-2: Role in asthma pathogenesis and utility as a biomarker of disease severity
Authors: Gandhi Vivek Dipak , Shrestha Palikhe Nami , Vliagoftis Harissios
Published: July 2022

PAR2, a receptor activated by serine proteases, has primarily pro-inflammatory roles in the airways and may play a role in asthma pathogenesis. PAR2 exerts its effects in the lungs through activation of a variety of airway cells, but also activation of circulating immune cells. There is evidence that PAR2 expression increases in asthma and other inflammatory diseases, although the regulation of PAR2 expression is not fully understood. Here we review the available literature on the potential role of PAR2 in asthma pathogenesis and propose a model of PAR2-mediated development of allergic sensitization. We also propose, based on our previous work, that PAR2 expression on peripheral blood monocyte subsets has the potential to serve as a biomarker of asthma severity and/or control.
Attenuation of HECT-E3 ligase expression rescued memory deficits in 3xTg-AD mice
Authors: Suresh Pavithra , Jasmin Sureka , Yen Yun , Hsu Hao-Jen , Varinthra Peeraporn , Pairojana Tanita , Chen Chien-Chang , Liu Ingrid Y.
Published: July 2022

Alzheimer's disease (AD) is one of the most common progressive neurodegenerative disorders that cause deterioration of cognitive functions. Recent studies suggested that the accumulation of inflammatory molecules and impaired protein degradation mechanisms might both play a critical role in the progression of AD. Autophagy is a major protein degradation pathway that can be controlled by several HECT-E3 ligases, which then regulates the expression of inflammatory molecules. E3 ubiquitin ligases are known to be upregulated in several neurodegenerative diseases. Here, we studied the expressional change of HECT-E3 ligase using M01 on autophagy and inflammasome pathways in the context of AD pathogenesis. Our results demonstrated that the M01 treatment reversed the working memory deficits in 3xTg-AD mice when examined with the T-maze and reversal learning with the Morris water maze. Additionally, the electrophysiology recordings indicated that M01 treatment enhanced the long-term potentiation in the hippocampus of 3xTg-AD mice. Together with the improved memory performance, the expression levels of the NLRP3 inflammasome protein were decreased. On the other hand, autophagy-related molecules were increased in the hippocampus of 3xTg-AD mice. Furthermore, the protein docking analysis indicated that the binding affinity of M01 to the WWP1 and NEDD4 E3 ligases was the highest among the HECT family members. The western blot analysis also confirmed the decreased expression level of NEDD4 protein in the M01-treated 3xTg-AD mice. Overall, our results demonstrate that the modulation of HECT-E3 ligase expression level can be used as a strategy to treat early memory deficits in AD by decreasing NLRP3 inflammasome molecules and increasing the autophagy pathway.
The detrimental effects of progression of retinal degeneration in the visual cortex
Authors: Bhattacharyya Anwesha
Published: July 2022

The leading cause of blindness in inherited and age-related retinal degeneration (RD) is the death of retinal photoreceptors such as rods and cones. The most prevalent form of RD is age-related macular degeneration (AMD) which affects the macula resulting in an irreversible loss of vision. The other is a heterogenous group of inherited disorders known as Retinitis Pigmentosa (RP) caused by the progressive loss of photoreceptors. Several approaches have been developed in recent years to artificially stimulate the remaining retinal neurons using optogenetics, retinal prostheses, and chemical photoswitches. However, the outcome of these strategies has been limited. The success of these treatments relies on the morphology, physiology, and proper functioning of the remaining intact structures in the downstream visual pathway. It is not completely understood what all alterations occur in the visual cortex during RD. In this review, I will discuss the known information in the literature about morphological and functional changes that occur in the visual cortex in rodents and humans during RD. The aim is to highlight the changes in the visual cortex that will be helpful for developing tools and strategies directed toward the restoration of high-resolution vision in patients with visual impairment.
Spinal Obstruction-Related vs. Craniocervical Junction-Related Syringomyelia: A Comparative Study
Authors: Yuan Chenghua , Guan Jian , Du Yueqi , Fang Zeyu , Wang Xinyu , Yao Qingyu , Zhang Can , Jia Shanhang , Liu Zhenlei , Wang Kai , Duan Wanru , Wang Xingwen , Wang Zuowei , Wu Hao , Chen Zan , Jian Fengzeng
Published: August 2022

Background

No prior reports have focused on spinal cord injury (SCI) characteristics or inflammation after destruction of the blood–spinal cord barrier by syringomyelia. This study aimed to determine the differences in syringomyelia-related central SCI between craniocervical junction (CCJ) syringomyelia and post-traumatic syringomyelia (PTS) before and after decompression.

Methods

In all, 106 CCJ, 26 CCJ revision and 15 PTS patients (mean history of symptoms, 71.5 ± 94.3, 88.9 ± 85.5, and 32.3 ± 48.9 months) between 2015 and 2019 were included. The symptom course was analyzed with the American Spinal Injury Association ASIA and Klekamp–Samii scoring systems, and neurological changes were analyzed by the Kaplan–Meier statistics. The mean follow-up was 20.7 ± 6.2, 21.7 ± 8.8, and 34.8 ± 19.4 months.

Results

The interval after injury was longer in the PTS group, but the natural history of syringomyelia was shorter (p = 0.0004 and 0.0173, respectively). The initial symptom was usually paraesthesia (p = 0.258), and the other main symptoms were hypoesthesia (p = 0.006) and abnormal muscle strength (p = 0.004), gait (p < 0.0001), and urination (p < 0.0001). SCI associated with PTS was more severe than that associated with the CCJ (p = 0.003). The cavities in the PTS group were primarily located at the thoracolumbar level, while those in the CCJ group were located at the cervical-thoracic segment at the CCJ. The syrinx/cord ratio of the PTS group was more than 75% (p = 0.009), and the intradural adhesions tended to be more severe (p < 0.0001). However, there were no significant differences in long-term clinical efficacy or peripheral blood inflammation markers (PBIMs) except for the red blood cell (RBC) count (p = 0.042).

Conclusion

PTS tends to progress faster than CCJ-related syringomyelia. Except for the RBC count, PBIMs showed no value in distinguishing the two forms of syringomyelia. The predictive value of the neutrophil-to-lymphocyte ratio for syringomyelia-related inflammation was negative except in the acute phase.
A novel multiple time-frequency sequential coding strategy for hybrid brain-computer interface
Authors: Yue Zan , Wu Qiong , Ren Shi-Yuan , Li Man , Shi Bin , Pan Yu , Wang Jing
Published: July 2022

Background

For brain-computer interface (BCI) communication, electroencephalography provides a preferable choice due to its high temporal resolution and portability over other neural recording techniques. However, current BCIs are unable to sufficiently use the information from time and frequency domains simultaneously. Thus, we proposed a novel hybrid time-frequency paradigm to investigate better ways of using the time and frequency information.

Method

We adopt multiple omitted stimulus potential (OSP) and steady-state motion visual evoked potential (SSMVEP) to design the hybrid paradigm. A series of pre-experiments were undertaken to study factors that would influence the feasibility of the hybrid paradigm and the interaction between multiple features. After that, a novel Multiple Time-Frequencies Sequential Coding (MTFSC) strategy was introduced and explored in experiments.

Results

Omissions with multiple short and long durations could effectively elicit time and frequency features, including the multi-OSP, ERP, and SSVEP in this hybrid paradigm. The MTFSC was feasible and efficient. The preliminary online analysis showed that the accuracy and the ITR of the nine-target stimulator over thirteen subjects were 89.04% and 36.37 bits/min.

Significance

This study first combined the SSMVEP and multi-OSP in a hybrid paradigm to produce robust and abundant time features for coding BCI. Meanwhile, the MTFSC proved feasible and showed great potential in improving performance, such as expanding the number of BCI targets by better using time information in specific stimulated frequencies. This study holds promise for designing better BCI systems with a novel coding method.
Histopathological modeling of status epilepticus-induced brain damage based on in vivo diffusion tensor imaging in rats
Authors: San Martín Molina Isabel , Salo Raimo A. , Gröhn Olli , Tohka Jussi , Sierra Alejandra
Published: July 2022

Non-invasive magnetic resonance imaging (MRI) methods have proved useful in the diagnosis and prognosis of neurodegenerative diseases. However, the interpretation of imaging outcomes in terms of tissue pathology is still challenging. This study goes beyond the current interpretation ofin vivodiffusion tensor imaging (DTI) by constructing multivariate models of quantitative tissue microstructure in status epilepticus (SE)-induced brain damage. We performedin vivoDTI and histology in rats at 79 days after SE and control animals. The analyses focused on the corpus callosum, hippocampal subfield CA3b, and layers V and VI of the parietal cortex. Comparison between control and SE rats indicated that a combination of microstructural tissue changes occurring after SE, such as cellularity, organization of myelinated axons, and/or morphology of astrocytes, affect DTI parameters. Subsequently, we constructed a multivariate regression model for explaining and predicting histological parameters based on DTI. The model revealed that DTI predicted well the organization of myelinated axons (cross-validated R = 0.876) and astrocyte processes (cross-validated R = 0.909) and possessed a predictive value for cell density (CD) (cross-validated R = 0.489). However, the morphology of astrocytes (cross-validated R > 0.05) was not well predicted. The inclusion of parameters from CA3b was necessary for modeling histopathology. Moreover, the multivariate DTI model explained better histological parameters than any univariate model. In conclusion, we demonstrate that combining several analytical and statistical tools can help interpret imaging outcomes to microstructural tissue changes, opening new avenues to improve the non-invasive diagnosis and prognosis of brain tissue damage.
Increased functional connectivity of white-matter in myotonic dystrophy type 1
Authors: Li Jing , Li Jie , Huang Pei , Huang Li-Na , Ding Qing-Guo , Zhan Linlin , Li Mengting , Zhang Jiaxi , Zhang Hongqiang , Cheng Lulu , Li Huayun , Liu Dong-Qiang , Zhou Hai-Yan , Jia Xi-Ze
Published: August 2022

Background

Myotonic dystrophy type 1 (DM1) is the most common and dominant inherited neuromuscular dystrophy disease in adults, involving multiple organs, including the brain. Although structural measurements showed that DM1 is predominantly associated with white-matter damage, they failed to reveal the dysfunction of the white-matter. Recent studies have demonstrated that the functional activity of white-matter is of great significance and has given us insights into revealing the mechanisms of brain disorders.

Materials and methods

Using resting-state fMRI data, we adopted a clustering analysis to identify the white-matter functional networks and calculated functional connectivity between these networks in 16 DM1 patients and 18 healthy controls (HCs). A two-sample t-test was conducted between the two groups. Partial correlation analyzes were performed between the altered white-matter FC and clinical MMSE or HAMD scores.

Results

We identified 13 white-matter functional networks by clustering analysis. These white-matter functional networks can be divided into a three-layer network (superficial, middle, and deep) according to their spatial distribution. Compared to HCs, DM1 patients showed increased FC within intra-layer white-matter and inter-layer white-matter networks. For intra-layer networks, the increased FC was mainly located in the inferior longitudinal fasciculus, prefrontal cortex, and corpus callosum networks. For inter-layer networks, the increased FC of DM1 patients is mainly located in the superior corona radiata and deep networks.

Conclusion

Results demonstrated the abnormalities of white-matter functional connectivity in DM1 located in both intra-layer and inter-layer white-matter networks and suggested that the pathophysiology mechanism of DM1 may be related to the white-matter functional dysconnectivity. Furthermore, it may facilitate the treatment development of DM1.
A fused biometrics information graph convolutional neural network for effective classification of patellofemoral pain syndrome
Authors: Xiong Baoping , OuYang Yaozong , Chang Yiran , Mao Guoju , Du Min , Liu Bijing , Xu Yong
Published: July 2022

Patellofemoral pain syndrome (PFPS) is a common, yet misunderstood, knee pathology. Early accurate diagnosis can help avoid the deterioration of the disease. However, the existing intelligent auxiliary diagnosis methods of PFPS mainly focused on the biosignal of individuals but neglected the common biometrics of patients. In this paper, we propose a PFPS classification method based on the fused biometrics information Graph Convolution Neural Networks (FBI-GCN) which focuses on both the biosignal information of individuals and the common characteristics of patients. The method first constructs a graph which uses each subject as a node and fuses the biometrics information (demographics and gait biosignal) of different subjects as edges. Then, the graph and node information [biosignal information, including the joint kinematics and surface electromyography (sEMG)] are used as the inputs to the GCN for diagnosis and classification of PFPS. The method is tested on a public dataset which contain walking and running data from 26 PFPS patients and 15 pain-free controls. The results suggest that our method can classify PFPS and pain-free with higher accuracy (mean accuracy = 0.8531 ± 0.047) than other methods with the biosignal information of individuals as input (mean accuracy = 0.813 ± 0.048). After optimal selection of input variables, the highest classification accuracy (mean accuracy = 0.9245 ± 0.034) can be obtained, and a high accuracy can still be obtained with a 40% reduction in test variables (mean accuracy = 0.8802 ± 0.035). Accordingly, the method effectively reflects the association between subjects, provides a simple and effective aid for physicians to diagnose PFPS, and gives new ideas for studying and validating risk factors related to PFPS.
Bioelectrical impedance vector analysis and body composition in cervical spinal cord injury: A pilot study
Authors: Bauermann Andreia , Costa e Silva Anselmo de Athayde , Figueiredo Flávia , Koury Josely Correa
Published: August 2022

Introduction

Body composition assessment in cervical spinal cord injury (c-SCI) individuals is important to monitor the fat free-mass (FFM) loss, due to immobilization, or gain, due to exercise practice. Single frequency bioelectrical impedance analysis (SF-BIA) is low in cost, simple and easy.

Objectives

The aims of this study are: to evaluate the concordance between the FFM values obtained using dual X-ray absorptiometry (DXA) and the three SF-BIA previous predictive equations; and to test the applicability of the bioelectrical impedance vector analysis (BIVA).

Methods

Twenty-three c-SCI males were divided into two groups: Physically active (PA; n = 13; at least 150 min/week) and non-active individuals (NPA) and were assessed by DXA and SF-BIA simultaneously.

Results

FFM values were similar between groups PA and NPA. Considering all participants, FFM values obtained by Kocina and Heyward (>11%) and Sun (<15.4%) predictive equations were different when compared to DXA (p < 0.01). However, Buchholz's et al. predictive equation showed FFM values similar to DXA, but presented poor concordance (<7%, p = 0.99; concordance coefficient = 0.85). BIVA showed consistency in ellipse distribution using FFM obtained using Buchholz et al. predictive equation.

Conclusions

The use of non-specific BIA equations can lead to misinterpretation in FFM values in male c-SCI individuals. Predictive equations for this group need to be developed.