bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of liver FGF21-KLB signaling in ketogenic diet-induced amelioration of hepatic steatosis
Authors: Guo Wanrong , Cao Huanyi , Shen Yunfeng , Li Wuguo , Wang Wei , Cheng Lidan , Cai Mengyin , Xu Fen
Published: April 2024

Background

The effectiveness of ketogenic diet (KD) in ameliorating fatty liver has been established, although its mechanism is under investigation. Fibroblast growth factor 21 (FGF21) positively regulates obesity-associated metabolic disorders and is elevated by KD. FGF21 conventionally initiates its intracellular signaling via receptor β-klotho (KLB). However, the mechanistic role of FGF21-KLB signaling for KD-ameliorated fatty liver remains unknown. This study aimed to delineate the critical role of FGF21 signaling in the ameliorative effects of KD on hepatic steatosis.

Methods

Eight-week-old C57BL/6 J mice were fed a chow diet (CD), a high-fat diet (HFD), or a KD for 16 weeks. Adeno-associated virus-mediated liver-specific KLB knockdown mice and control mice were fed a KD for 16 weeks. Phenotypic assessments were conducted during and after the intervention. We investigated the mechanism underlying KD-alleviated hepatic steatosis using multi-omics and validated the expression of key genes.

Results

KD improved hepatic steatosis by upregulating fatty acid oxidation and downregulating lipogenesis. Transcriptional analysis revealed that KD dramatically activated FGF21 pathway, including KLB and fibroblast growth factor receptor 1 (FGFR1). Impairing liver FGF21 signaling via KLB knockdown diminished the beneficial effects of KD on ameliorating fatty liver, insulin resistance, and regulating lipid metabolism.

Conclusion

KD demonstrates beneficial effects on diet-induced metabolic disorders, particularly on hepatic steatosis. Liver FGF21-KLB signaling plays a critical role in the KD-induced amelioration of hepatic steatosis.
Infection with SARS-CoV-2 can cause pancreatic impairment
Authors: Deng Wei , Bao Linlin , Song Zhiqi , Zhang Ling , Yu Pin , Xu Yanfeng , Wang Jue , Zhao Wenjie , Zhang Xiuqin , Han Yunlin , Li Yanhong , Liu Jiangning , Lv Qi , Liang Xujian , Li Fengdi , Qi Feifei , Deng Ran , Wang Siyuan , Xiong Yibai , Xiao Ruiping , Wang Hongyang , Qin Chuan
Published: April 2024

Evidence suggests associations between COVID-19 patients or vaccines and glycometabolic dysfunction and an even higher risk of the occurrence of diabetes. Herein, we retrospectively analyzed pancreatic lesions in autopsy tissues from 67 SARS-CoV-2 infected non-human primates (NHPs) models and 121 vaccinated and infected NHPs from 2020 to 2023 and COVID-19 patients. Multi-label immunofluorescence revealed direct infection of both exocrine and endocrine pancreatic cells by the virus in NHPs and humans. Minor and limited phenotypic and histopathological changes were observed in adult models. Systemic proteomics and metabolomics results indicated metabolic disorders, mainly enriched in insulin resistance pathways, in infected adult NHPs, along with elevated fasting C-peptide and C-peptide/glucose ratio levels. Furthermore, in elder COVID-19 NHPs, SARS-CoV-2 infection causes loss of beta (β) cells and lower expressed-insulin in situ characterized by islet amyloidosis and necrosis, activation of α-SMA and aggravated fibrosis consisting of lower collagen in serum, an increase of pancreatic inflammation and stress markers, ICAM-1 and G3BP1, along with more severe glycometabolic dysfunction. In contrast, vaccination maintained glucose homeostasis by activating insulin receptor α and insulin receptor β. Overall, the cumulative risk of diabetes post-COVID-19 is closely tied to age, suggesting more attention should be paid to blood sugar management in elderly COVID-19 patients.
A life cycle analysis of the environmental impact of procurement, waste and water in the dental practice
Authors: Suresh Peter , Crotty John , Tesanovic Sonja , Alaweed Othman , Doyle Sadhbh , Kiandee Mikra , Hayes Emily , Umeh Vanessa , Khalilinejad Bita , Duane Brett
Published: April 2024

Background Health care is a significant contributor to climate change. Global pressure for a change towards a more sustainable way of providing dental health care has resulted in the creation of the Green Impact Toolkit, which is comprised of a list of suggested changes that dental practices can make to become more sustainable in a number of categories, such as procurement, waste and water.

Aims To compare the effectiveness of changes suggested by the Green Impact Toolkit.

Materials and methods A comparative life cycle assessment (LCA) was conducted using the Ecoinvent database v3.8 and these data were processed using OpenLCA v1.10.3 software.

Results The carbon footprint per patient was significantly reduced after the recommendations were implemented. For instance, using water from a rainwater collection tank instead of the mains supply saved 30 g CO2eq (carbon dioxide equivalents) per patient, a 90% reduction in carbon footprint.

Discussion This comparative LCA identified some effective changes which can be easily made by a dental practice. Nevertheless, some actions require some initial financial investment and may be difficult to implement in a busy modern dental practice setting.

Conclusion The findings from this study can be used to guide dental practices to making choices which are more sustainable and eco-friendly in the future.
A bone adhesive enhances osteoporotic fracture repair by regulating bone homeostasis
Authors: Su Shenghui , Xie Jiajun , Gao Jian , Liu Shencai , Dong Xieping , Li Jianwei , Gao Zhong Feng , Chen Keyuan , Liu Weilu
Published: April 2024

Patients suffering from osteoporotic fractures often require effective fixation and subsequent bone repair. However, the currently available materials are functionally limited and often fail to improve outcomes in this patient population. In this study, we developed orthopedic adhesives doped with romosozumab-loaded mesoporous bioactive glass nanoparticles to aid in osteoporotic fracture fixation and restore dysregulated bone homeostasis. These adhesives were designed to promote osteoblast formation while simultaneously inhibiting osteoclastic bone-resorbing activity, thus working synergistically to promote the healing of osteoporotic fractures. Orthopedic adhesives exhibit injectability, reversible adhesiveness, and malleability, enhancing their adaptability to complex clinical scenarios. Furthermore, the release of romosozumab from mesoporous bioactive glass nanoparticles accelerated osteogenesis and inhibited osteoclastogenesis, delaying the bone resorption process. This dual action contributes to the regulation of bone regeneration and remodeling. Notably, our orthopedic adhesive could restore the disrupted bone homeostasis associated with osteoporotic fractures.
Origami metamaterials for ultra-wideband and large-depth reflection modulation
Authors: Song Zicheng , Zhu Juan-Feng , Wang Xianchao , Zhang Ruicong , Min Pingping , Cao Wenxin , He Yurong , Han Jiecai , Wang Tianyu , Zhu Jiaqi , Wu Lin , Qiu Cheng-Wei
Published: April 2024

The dynamic control of electromagnetic waves is a persistent pursuit in modern industrial development. The state-of-the-art dynamic devices suffer from limitations such as narrow bandwidth, limited modulation range, and expensive features. To address these issues, we fuse origami techniques with metamaterial design to achieve ultra-wideband and large-depth reflection modulation. Through a folding process, our proposed metamaterial achieves over 10-dB modulation depth over 4.96 – 38.8 GHz, with a fractional bandwidth of 155% and tolerance to incident angles and polarizations. Its ultra-wideband and large-depth reflection modulation performance is verified through experiments and analyzed through multipole decomposition theory. To enhance its practical applicability, transparent conductive films are introduced to the metamaterial, achieving high optical transparency (>87%) from visible to near-infrared light while maintaining cost-effectiveness. Benefiting from lightweight, foldability, and low-cost properties, our design shows promise for extensive satellite communication and optical window mobile communication management.
Integrated proteogenomic and metabolomic characterization of papillary thyroid cancer with different recurrence risks
Authors: Qu Ning , Chen Di , Ma Ben , Zhang Lijun , Wang Qiuping , Wang Yuting , Wang Hongping , Ni Zhaoxian , Wang Wen , Liao Tian , Xiang Jun , Wang Yulong , Jin Shi , Xue Dixin , Wu Weili , Wang Yu , Ji Qinghai , He Hui , Piao Hai-long , Shi Rongliang
Published: April 2024

Although papillary thyroid cancer (PTC) has a good prognosis, its recurrence rate is high and remains a core concern in the clinic. Molecular factors contributing to different recurrence risks (RRs) remain poorly defined. Here, we perform an integrative proteogenomic and metabolomic characterization of 102 Chinese PTC patients with different RRs. Genomic profiling reveals that mutations in MUC16 and TERT promoter as well as multiple gene fusions like NCOA4-RET are enriched by the high RR. Integrative multi-omics analyses further describe the multi-dimensional characteristics of PTC, especially in metabolism pathways, and delineate dominated molecular patterns of different RRs. Moreover, the PTC patients are clustered into four subtypes (CS1: low RR and BRAF-like; CS2: high RR and metabolism type, worst prognosis; CS3: high RR and immune type, better prognosis; CS4: high RR and BRAF-like) based on the omics data. Notably, the subtypes display significant differences considering BRAF and TERT promoter mutations, metabolism and immune pathway profiles, epithelial cell compositions, and various clinical factors (especially RRs and prognosis) as well as druggable targets. This study can provide insights into the complex molecular characteristics of PTC recurrences and help promote early diagnosis and precision treatment of recurrent PTC.
Evidence for a competitive relationship between executive functions and statistical learning
Authors: Pedraza Felipe , Farkas Bence C. , Vékony Teodóra , Haesebaert Frederic , Phelipon Romane , Mihalecz Imola , Janacsek Karolina , Anders Royce , Tillmann Barbara , Plancher Gaën , Németh Dezső
Published: April 2024

The ability of the brain to extract patterns from the environment and predict future events, known as statistical learning, has been proposed to interact in a competitive manner with prefrontal lobe-related networks and their characteristic cognitive or executive functions. However, it remains unclear whether these cognitive functions also possess a competitive relationship with implicit statistical learning across individuals and at the level of latent executive function components. In order to address this currently unknown aspect, we investigated, in two independent experiments (NStudy1 = 186, NStudy2 = 157), the relationship between implicit statistical learning, measured by the Alternating Serial Reaction Time task, and executive functions, measured by multiple neuropsychological tests. In both studies, a modest, but consistent negative correlation between implicit statistical learning and most executive function measures was observed. Factor analysis further revealed that a factor representing verbal fluency and complex working memory seemed to drive these negative correlations. Thus, the antagonistic relationship between implicit statistical learning and executive functions might specifically be mediated by the updating component of executive functions or/and long-term memory access.
BepiColombo observations of cold oxygen and carbon ions in the flank of the induced magnetosphere of Venus
Authors: Hadid L. Z. , Delcourt D. , Saito Y. , Fränz M. , Yokota S. , Fiethe B. , Verdeil C. , Katra B. , Leblanc F. , Fischer H. , Persson M. , Aizawa S. , André N. , Harada Y. , Fedorov A. , Fontaine D. , Krupp N. , Michalik H. , Berthelier J-J. , Krüger H. , Murakami G. , Matsuda S. , Heyner D. , Auster H.-U. , Richter I. , Mieth J. Z. D. , Schmid D. , Fischer D.
Published: April 2024

On 10 August 2021, the Mercury-bound BepiColombo spacecraft performed its second fly-by of Venus and provided a short-lived observation of its induced magnetosphere. Here we report results recorded by the Mass Spectrum Analyzer on board Mio, which reveal the presence of cold O+ and C+ with an average total flux of ~4 ± 1 × 104 cm−2 s−1 at a distance of about six planetary radii in a region that has never been explored before. The ratio of escaping C+ to O+ is at most 0.31 ± 0.2, implying that, in addition to atomic O+ ions, CO group ions or water group ions may be a source of the observed O+. Simultaneous magnetometer observations suggest that these planetary ions were in the magnetosheath flank in the vicinity of the magnetic pileup boundary downstream. These results have important implications regarding the evolution of Venus’s atmosphere and, in particular, the evolution of water on the surface of the planet.
Rapid Laurentide Ice Sheet growth preceding the Last Glacial Maximum due to summer snowfall
Authors: Niu Lu , Knorr Gregor , Krebs-Kanzow Uta , Gierz Paul , Lohmann Gerrit
Published: April 2024

There has been extensive research into the nonlinear responses of the Earth system to astronomical forcing during the last glacial cycle. However, the speed and spatial geometry of ice sheet expansion to its largest extent at the Last Glacial Maximum 21 thousand years ago remains uncertain. Here we use an Earth system model with interactive ice sheets to show that distinct initial North American (Laurentide) ice sheets at 38 thousand years ago converge towards a configuration consistent with the Last Glacial Maximum due to feedbacks between atmospheric circulation and ice sheet geometry. Notably, ice advance speed and spatial pattern in our model are controlled by the amount of summer snowfall, which is dependent on moisture transport pathways from the North Atlantic warm pool linked to ice sheet geometry. The consequence of increased summer snowfall on the surface mass balance of the ice sheet is not only the direct increase in accumulation but the indirect reduction in melt through the snow/ice–albedo feedback. These feedbacks provide an effective mechanism for ice growth for a range of initial ice sheet states and may explain the rapid North American ice volume increase during the last ice age and potentially driving growth during previous glacial periods.
Structure of the native γ-tubulin ring complex capping spindle microtubules
Authors: Dendooven Tom , Yatskevich Stanislau , Burt Alister , Chen Zhuo A. , Bellini Dom , Rappsilber Juri , Kilmartin John V. , Barford David
Published: April 2024

Microtubule (MT) filaments, composed of α/β-tubulin dimers, are fundamental to cellular architecture, function and organismal development. They are nucleated from MT organizing centers by the evolutionarily conserved γ-tubulin ring complex (γTuRC). However, the molecular mechanism of nucleation remains elusive. Here we used cryo-electron tomography to determine the structure of the native γTuRC capping the minus end of a MT in the context of enriched budding yeast spindles. In our structure, γTuRC presents a ring of γ-tubulin subunits to seed nucleation of exclusively 13-protofilament MTs, adopting an active closed conformation to function as a perfect geometric template for MT nucleation. Our cryo-electron tomography reconstruction revealed that a coiled-coil protein staples the first row of α/β-tubulin of the MT to alternating positions along the γ-tubulin ring of γTuRC. This positioning of α/β-tubulin onto γTuRC suggests a role for the coiled-coil protein in augmenting γTuRC-mediated MT nucleation. Based on our results, we describe a molecular model for budding yeast γTuRC activation and MT nucleation.
A dataset for soil organic carbon in agricultural systems for the Southeast Asia region
Authors: Gomez Federico , Carcedo Ana , Mean Chan Makara , Reyes Manuel , Hok Lyda , Tivet Florent , Seng Vang , Vara Prasad P. V. , Ciampitti Ignacio
Published: April 2024

The determination of changes in soil organic carbon (SOC) content under different cropping systems is necessary for policy development oriented towards soil conservation, C sequestration, and future C credit markets. The aim of this study was to generate an open SOC dataset resulting from a systematic literature search related to the agricultural systems for Southeast Asia. The dataset has 209 articles and 4341 observations on soils of cropping systems in this region from articles published between 1987 and 2023. This dataset included different management practices, land uses, soil sampling depth, and length of SOC content assessment. In addition, inherent features of crop production reported in the experiments were included in the dataset. This dataset can be applied to quantify and compare the impact of different land uses or management practices on SOC content, providing foundational knowledge towards identifying sustainable practices. Lastly, it is a useful guide for future regional SOC sequestration policies and the development of C credit markets.
The transcriptome assembly of the European freshwater mussel Unio elongatulus C. Pfeiffer, 1825
Authors: Gomes-dos-Santos André , Fonseca Elza , Riccardi Nicoletta , Hinzmann Mariana , Lopes-Lima Manuel , Froufe Elsa
Published: April 2024

Freshwater mussels of the order Unionida are a global conservation concern. Species of this group are strictly freshwater, sessile, slow-growing animals and, extremely sensitive to environmental changes. Human-mediated changes in freshwater habitats are imposing enormous pressure on the survival of freshwater mussels. Although a few flagship species are protected in Europe, other highly imperilled species receive much less attention. Moreover, knowledge about biology, ecology, and evolution and proper conservation assessments of many European species are still sparse. This knowledge gap is further aggravated by the lack of genomic resources available, which are key tools for conservation. Here we present the transcriptome assembly of Unio elongatulus C. Pfeiffer, 1825, one of the least studied European freshwater mussels. Using the individual sequencing outputs from eight physiologically representative mussel tissues, we provide an annotated panel of tissue-specific Relative Gene Expression profiles. These resources are pivotal to studying the species’ biological and ecological features, as well as helping to understand its vulnerability to current and future threats.
Validation of the 2022 European LeukemiaNet risk stratification for acute myeloid leukemia
Authors: Song Ga-Young , Kim Hyeon-Jong , Kim TaeHyung , Ahn Seo-Yeon , Jung Sung-Hoon , Kim Mihee , Yang Deok-Hwan , Lee Je-Jung , Kim Mi Yeon , Cheong June-Won , Jung Chul Won , Jang Jun Ho , Kim Hee- Je , Moon Joon Ho , Sohn Sang Kyun , Won Jong-Ho , Park Seong Kyu , Kim Sung-Hyun , Choi Chang Kyun , Kim Hyeoung-Joon , Ahn Jae-Sook , Kim Dennis Dong Hwan
Published: April 2024

This study aimed to validate the 2022 European LeukemiaNet (ELN) risk stratification for acute myeloid leukemia (AML). A total of 624 newly diagnosed AML patients from 1998 to 2014 were included in the analysis. Genetic profiling was conducted using targeted deep sequencing of 45 genes based on recurrent driver mutations. In total, 134 (21.5%) patients had their risk classification reassessed according to the 2022 ELN risk stratification. Among those initially classified as having a favorable risk in 2017 (n = 218), 31 and 3 patients were reclassified as having intermediate risk or adverse risk, respectively. Among the three subgroups, the 2022 ELN favorable-risk group showed significantly longer survival outcomes than the other groups. Within the 2017 ELN intermediate-risk group (n = 298), 21 and 46 patients were reclassified as having favorable risk or adverse risk, respectively, and each group showed significant stratifications in survival outcomes. Some patients initially classified as having adverse risk in 2017 were reclassified into the intermediate-risk group (33 of 108 patients), but no prognostic improvements were observed in this group. A multivariable analysis identified the 2022 ELN risk stratification, age, and receiving allogeneic hematopoietic cell transplantation as significant prognostic factors for survival. The 2022 ELN risk stratification enables more precise decisions for proceeding with allogeneic hematopoietic cell transplantation for AML patients.
Shape variation and sex differences of the adult human mandible evaluated by geometric morphometrics
Authors: Chalazoniti Aspasia , Lattanzi Wanda , Halazonetis Demetrios J.
Published: April 2024

In cases of osseous defects, knowledge of the anatomy, and its age and sex-related variations, is essential for reconstruction of normal morphology. Here, we aimed at creating a 3D atlas of the human mandible in an adult sample using dense landmarking and geometric morphometrics. We segmented 50 male and 50 female mandibular surfaces from CBCT images (age range: 18.9–73.7 years). Nine fixed landmarks and 510 sliding semilandmarks were digitized on the mandibular surface, and then slid by minimizing bending energy against the average shape. Principal component analysis extracted the main patterns of shape variation. Sexes were compared with permutation tests and allometry was assessed by regressing on the log of the centroid size. Almost 49 percent of shape variation was described by the first three principal components. Shape variation was related to width, height and length proportions, variation of the angle between ramus and corpus, height of the coronoid process and inclination of the symphysis. Significant sex differences were detected, both in size and shape. Males were larger than females, had a higher ramus, more pronounced gonial angle, larger inter-gonial width, and more distinct antegonial notch. Accuracy of sexing based on the first two principal components in form space was 91 percent. The degree of edentulism was weakly related to mandibular shape. Age effects were not significant. The resulting atlas provides a dense description of mandibular form that can be used clinically as a guide for planning surgical reconstruction.
Association of dietary intake of B vitamins with glaucoma
Authors: Hou Jingjing , Wen Yu , Gao Sijia , Jiang Zhengxuan , Tao Liming
Published: April 2024

This cross-sectional study investigated the association between glaucoma and B vitamin dietary intake. A total of 5025 enrolled individuals participated in self-reported glaucoma questionnaire and 3264 participated in International Society Geographical and Epidemiological Ophthalmology (ISGEO) criteria. In self-reported glaucoma, the risk of having self-reported glaucoma was lower in the third quartile of vitamin B1 intake (odds ratio [odds ratio [OR] 0.63, 95% confidence interval [CI] 0.40–0.97), and P trend (P trend = 0.004) for vitamin B12 was significant; in males, the third quartile of vitamin B1 intake (OR 0.44, 95% CI 0.24–0.83) and the fourth quartile of vitamin B2 intake (OR 0.39, 95% CI 0.17–0.89) were associated with a lower risk. In glaucoma based on ISGEO criteria, the increase of niacin intake (OR 0.94, 95% CI 0.89–0.99) was negatively associated with the odds of self-reported glaucoma. After sex-stratified analysis, the third quartile of vitamin B6 intake (OR 0.21, 95% CI 0.08–0.60) in males were associated with reduced odds of glaucoma. The restricted cubic spline analysis revealed a nonlinear association of vitamin B2 (p for nonlinearity = 0.04) and B9 (p for nonlinearity = 0.024) intake with glaucoma diagnosed by ISGEO criteria in females.
Effect of a single immersion in cold water below 4 °C on haemorheological properties of blood in healthy men
Authors: Teległów Aneta , Genç Hatice , Cicha Iwona
Published: April 2024

Cold water immersion (CWI) involves rapid cooling of the body, which, in healthy individuals, triggers a defence response to an extreme stimulus, to which the body reacts with stress. The aim of the study was to determine the effect of CWI on hemorheological blood indicators. The study group consisted of 13 young males. Blood samples were collected before and after CWI. The assessed parameters included the complete blood count, fibrinogen, hs-C-reactive protein (CRP), proteinogram, and blood rheology factors, such as erythrocyte elongation index (EI), half-time of total aggregation, and aggregation index. Additionally, the effect of reduced temperature on primary human vascular endothelium was investigated in vitro. CWI resulted in the decrease of body temperature to 31.55 ± 2.87 °C. After CWI, neutrophil count and mean corpuscular volume (MCV) were significantly increased in the study group, while lymphocyte count was significantly decreased. Significantly higher levels of total blood protein and albumin concentration were detected after the immersion. Among hemorheological characteristics, erythrocyte EIs at shear stress values ranging from 2.19 to 60.30 Pa were significantly lower after CWI. No significant changes in other rheological, morphological or biochemical parameters were observed. In vitro, human umbilical vein endothelial cells responded to 3 h of temperature decrease to 25 °C with unchanged viability, but increased recruitment of THP-1 monocytic cells and changes in cell morphology were observed. This was the first study to evaluate the effect of single CWI on rheological properties of blood in healthy young men. The results indicate that a single CWI may increase blood protein concentrations and worsen erythrocyte deformability parameters.
Advancing pathogen surveillance by nanopore sequencing and genotype characterization of Acheta domesticus densovirus in mass-reared house crickets
Authors: Lim Fang Shiang , González-Cabrera Joel , Keilwagen Jens , Kleespies Regina G. , Jehle Johannes A. , Wennmann Jörg T.
Published: April 2024

Rapid and reliable detection of pathogens is crucial to complement the growing industry of mass-reared insects, in order to safeguard the insect colonies from outbreak of diseases, which may cause significant economic loss. Current diagnostic methods are mainly based on conventional PCR and microscopic examination, requiring prior knowledge of disease symptoms and are limited to identifying known pathogens. Here, we present a rapid nanopore-based metagenomics approach for detecting entomopathogens from the European house cricket (Acheta domesticus). In this study, the Acheta domesticus densovirus (AdDV) was detected from diseased individuals using solely Nanopore sequencing. Virus reads and genome assemblies were obtained within twenty-four hours after sequencing. Subsequently, due to the length of the Nanopore reads, it was possible to reconstruct significantly large parts or even the entire AdDV genome to conduct studies for genotype identification. Variant analysis indicated the presence of three AdDV genotypes within the same house cricket population, with association to the vital status of the diseased crickets. This contrast provided compelling evidence for the existence of non-lethal AdDV genotypes. These findings demonstrated nanopore-based metagenomics sequencing as a powerful addition to the diagnostic tool kit for routine pathogen surveillance and diagnosis in the insect rearing industry.
Genetic interference with HvNotch provides new insights into the role of the Notch-signalling pathway for developmental pattern formation in Hydra
Authors: Pan Qin , Mercker Moritz , Klimovich Alexander , Wittlieb Jörg , Marciniak-Czochra Anna , Böttger Angelika
Published: April 2024

The Notch-signalling pathway plays an important role in pattern formation in Hydra. Using pharmacological Notch inhibitors (DAPT and SAHM1), it has been demonstrated that HvNotch is required for head regeneration and tentacle patterning in Hydra. HvNotch is also involved in establishing the parent-bud boundary and instructing buds to develop feet and detach from the parent. To further investigate the functions of HvNotch, we successfully constructed NICD (HvNotch intracellular domain)-overexpressing and HvNotch-knockdown transgenic Hydra strains. NICD-overexpressing transgenic Hydra showed a pronounced inhibition on the expression of predicted HvNotch-target genes, suggesting a dominant negative effect of ectopic NICD. This resulted in a “Y-shaped” phenotype, which arises from the parent-bud boundary defect seen in polyps treated with DAPT. Additionally, “multiple heads”, “two-headed” and “ectopic tentacles” phenotypes were observed. The HvNotch-knockdown transgenic Hydra with reduced expression of HvNotch exhibited similar, but not identical phenotypes, with the addition of a “two feet” phenotype. Furthermore, we observed regeneration defects in both, overexpression and knockdown strains. We integrated these findings into a mathematical model based on long-range gradients of signalling molecules underlying sharply defined positions of HvNotch-signalling cells at the Hydra tentacle and bud boundaries.
Repeated-sprint sets test: a new method for evaluating and forecasting fitness in elite young male soccer players
Authors: Selmi Mohamed Amin , Ceylan Halil ibrahim , Hammami Raouf , Sassi Radhouane Haj , González-Fernández Francisco Tomás , Morgans Ryland , Bragazzi Nicola Luigi
Published: April 2024

The objective of the current study was to explore the correlation between repeated sprint sets (RSS) ability and several physical attributes, including maximum sprint speed, maximal aerobic speed, maximal anaerobic speed, aerobic capacity, and explosive strength. Moreover, the aim was to assess the suitability of RSS as a comprehensive evaluation tool for physical qualities and to determine which physical field tests most accurately predict RSS in elite young male soccer players. A total of thirty-two young elite male soccer players (mean age 14.6 ± 0.3 years; predicted years from peak height velocity (PHV): − 0.4 ± 0.3; years in training: 3.7 ± 0.5) voluntarily participated in the study. The players participated in eight consecutive specific physical tests, with a minimum 72-h recovery between each session to minimize the impact of fatigue during the second trial. The participants completed the tests in the following order: RSS test, Vam-Eval test, a constant velocity test performed until exhaustion at 100% of vVO2max (tlim100), 20-m Multi-Stage Shuttle Run test (VMSRT), Yo-Yo Intermittent Recovery Test level 1 (Yo-Yo IR1), Maximal Anaerobic Shuttle Running Test (VMASRT), Maximal Sprinting Speed Test (20-m flying sprint), Countermovement Jump (CMJ), and Standing Long Jump test (SLJ). The results of the study showed that there were very large negative correlations between tlim100 and SST (sum of sprint times), and large negative correlations between Yo-Yo IR1, Vam-Eval, and SST during RSS in young elite male soccer players (p < 0.05). Additionally, VMASRT and SLJ demonstrated a moderate negative correlation with SST (p < 0.05). In contrast, significant positive correlations were found between 20-m flying sprint and the SST (p < 0.05). According to the stepwise multiple linear regression analysis, the primary predictors of SST, ranked by importance, were tlim100 and Yo-Yo IR1. These two predictors collectively accounted for 72% of the variance in players’ SST (p < 0.0001). Due to the importance of aerobic capacity and short repeated accelerations/sprint sets for overall competitive performance in soccer, in conclusion, our results suggest that elite young male soccer players should perform both high intensity interval training and aeorobic capactity exercises as part of soccer training if the primary outcome is to improve repeated sprint ability performance.
Composition, characteristics, and treatment technologies of condensable particulate matter present in flue gas emitted by coking plants in China
Authors: Wang Chunyan , Du Yuhong , Yan Bo , Dong Yonggang , Zhao Zhihui , Shen Jinchao , Guo Mengxia , Zhang Zhaochi
Published: April 2024

To study the total particulate matter (TPM) in flue gas emitted by coking plants, a sampling system that could be used to collect filterable particulate matter (FPM) and condensable particulate matter (CPM) was designed and developed based on Method 202 recommended by the U.S. Environmental Protection Agency in 2017 and HJ 836-2017 issued by China. Using this system, FPM and CPM in flue gas emitted by four coking furnaces named A, B, C, and D were tested in China. Further, 9 water-soluble ions, 20 elements, and organic matter present in the CPM were simultaneously examined to determine their formation mechanisms. Statistical data suggested that the FPM emission level in the coking flue gas was low and the average mass concentration was less than 10 mg/m3. However, with high CPM and TPM emission levels, the TPM mass concentrations of A, B, C, and D were 130 ± 11.1, 84.4 ± 6.36, 35.1 ± 17.0, and 63.8 ± 13.0 mg/m3, respectively. The main component of TPM was CPM, and the average mass concentration of CPM accounted for 98%, 95%, 68%, and 95% of TPM in furnaces A, B, C, and D, respectively. Water-soluble ions were the important components of CPM, and the total concentration of water-soluble ions accounted for 70%, 87%, 42%, and 66% of CPM in furnaces A, B, C, and D, respectively. Toxic and harmful heavy metals, such as Mn, Cr, Ni, Cu, Zn, As, Cd, and Pb, were detected in CPM. The formation mechanism of CPM was analyzed in combination with flue-gas treatment. It was shown that the treatment process “activated carbon– flue-gas countercurrent-integrated purification technology + ammonia spraying” used in furnaces A and B was less effective in removing CPM, water-soluble ions, metals, and compounds than that of “selective catalytic reduction denitrification + limestone–gypsum wet desulfurization (spraying NaOH solution)” in furnaces C and D. Hence, different flue-gas treatment technologies and operation levels played vital roles in the formation, transformation, and removal of CPM from flue-gas. Organic components in CPM discharged from furnace A were determined via gas chromatography–mass spectrometry, and the top 15 organic components in CPM were obtained using the area normalization method. N-alkanes accounted for the highest proportion, followed by esters and phenols, and most of them were toxic and harmful to humans and ecosystems. Therefore, advanced CPM treatment technologies should be developed to reduce atmospheric PM2.5 and its precursors to improve ambient air quality in China.
The association between tobacco smoking and systolic toe pressures in active foot ulceration
Authors: Bechara Nada , Hng Tien-Ming , Gunton Jenny E.
Published: April 2024

Smoking may increase the risk of diabetic foot disease and ulceration. It does so by impairing glycaemic control and promoting the formation of advanced glycated end-products. Additionally, smoking is known to delay surgical wound healing and accelerate peripheral arterial disease. We aimed to determine whether toe pressures differed in smokers with a foot ulcer, when compared to non-smokers and ex-smokers, as well as ulcer outcomes at 12 months, among patients attending Blacktown Hospital High Risk Foot Service (HRFS). This study is a retrospective analysis of our prospectively collected clinic database. Eligible participants were adults attending the HRFS between June 2020 and April 2022. Participants were included if they had an ulcer, at least one systolic toe pressure reading completed at their initial visit and attended at least one follow-up visit. Participants were followed until healing, loss to follow-up or a minimum of 12 months. A total of 195 participants were included; 36 smokers, 82 ex-smokers, and 77 controls who had never smoked. Smoking status was by self-report. Current smokers were significantly younger at initial presentation (p = .002) and tended towards lower socioeconomic status (p = .067). Current smokers were significantly more likely to have ischaemic grade 3 toe pressures (< 30 mmHg) of their left foot (p = .027), suggestive of reduced perfusion. At the end of follow up period, smokers had the numerically highest rates of minor amputations. In conclusion, smokers ulcerate younger and are more likely to have grade 3 ischaemia. Collecting information about the brachial artery pressures and the time since the last cigarette may clarify any relationship between smoking and toe pressures.

Trial registration: WSLHD HREC ethics approval 2111-02 and ANZCTR registration 382470. Registered on 15/09/2021.
Mood variability during adolescent development and its relation to sleep and brain development
Authors: Toenders Yara J. , van der Cruijsen Renske , Runze Jana , van de Groep Suzanne , Wierenga Lara , Crone Eveline A.
Published: April 2024

Mood swings, or mood variability, are associated with negative mental health outcomes. Since adolescence is a time when mood disorder onset peaks, mood variability during this time is of significant interest. Understanding biological factors that might be associated with mood variability, such as sleep and structural brain development, could elucidate the mechanisms underlying mood and anxiety disorders. Data from the longitudinal Leiden self-concept study (N = 191) over 5 yearly timepoints was used to study the association between sleep, brain structure, and mood variability in healthy adolescents aged 11–21 at baseline in this pre-registered study. Sleep was measured both objectively, using actigraphy, as well as subjectively, using a daily diary self-report. Negative mood variability was defined as day-to-day negative mood swings over a period of 5 days after an MRI scan. It was found that negative mood variability peaked in mid-adolescence in females while it linearly increased in males, and average negative mood showed a similar pattern. Sleep duration (subjective and objective) generally decreased throughout adolescence, with a larger decrease in males. Mood variability was not associated with sleep, but average negative mood was associated with lower self-reported energy. In addition, higher thickness in the dorsolateral prefrontal cortex (dlPFC) compared to same-age peers, suggesting a delayed thinning process, was associated with higher negative mood variability in early and mid-adolescence. Together, this study provides an insight into the development of mood variability and its association with brain structure.
Examining the relationship between self-efficacy, career development, and subjective wellbeing in physical education students
Authors: Yiming Yikeranmu , Shi Bing , Kayani Sumaira , Biasutti Michele
Published: April 2024

We investigated the relationship between self-efficacy and career development via subjective well-being of students majoring in physical education. Life satisfaction, positive affect, and negative affect were the componennts of subjective well-being. Participants were the 1381 adolescents with major in physical education with an age range of 18–22 years (Mage = 19.5 ± 1; females = 34.76%). Hayes PROCESS model was used to develop a multiple mediation model. The results suggest that higher self-efficacy leads to better career development. Further, a significant mediating role was played by negative and positive affect in case of the relationship between self-efficacy and career exploration, but life-satisfaction is not significant mediator. Conversely, life satisfaction and positive affect are significant mediators between self-efficacy and career adaptability but negative affect is not. The findings suggest that self-efficacy and subjective well-being benefit career development of adolescents in the physical education field.
Serum concentration of polychlorinated biphenyls and the risk of type 2 diabetes: a 10-year follow-up historical cohort study
Authors: Ravanipour Masoumeh , Nabipour Iraj , Yunesian Masud , Rastkari Noushin , Mahvi Amir Hossein
Published: April 2024

This study investigated the association between serum concentrations of Polychlorinated Biphenyls (PCBs) and the risk of type 2 diabetes within the general population. A ten-year follow-up historical cohort study was conducted during 2009–2019 as part of the Bushehr MONICA cohort study in Iran. Of 893 non-diabetes participants at base line, 181 individuals were included in the study. The concentration of nine PCB congeners was measured in individuals’ serum samples at baseline, and the risk of type 2 diabetes was determined based on fasting blood sugar at the end of follow-up. Multiple logistic regression models were used to assess the study outcomes after adjusting for covariates. This study included 59 diabetes individuals (32.6%; mean [SD] age: 58.64 [8.05]) and 122 non-diabetes individuals (67.4%; mean [SD] age: 52.75 [8.68]). Multivariable analysis revealed that a one-tertile increase (increasing from 33rd centile to 67th centile) in Σ non-dioxin-like-PCBs (OR 2.749, 95% CI 1.066–7.089), Σ dioxin-like-PCBs (OR 4.842, 95% CI 1.911–12.269), and Σ PCBs (OR 2.887, 95% CI 1.120–7.441) significantly associated with an increased risk of type 2 diabetes. The strongest association was obtained for dioxin-like PCBs. The results highlight a significant correlation between PCB exposure and an increased risk of type 2 diabetes. The evidence suggests that additional epidemiological studies are necessary to clarify the link between PCBs and diabetes.
Human influence on the recent weakening of storm tracks in boreal summer
Authors: Chemke Rei , Coumou Dim
Published: April 2024

Anthropogenic warming can alter large-scale circulation patterns in the atmosphere, which could have serious consequences for regional climate impacts and extreme weather. Observed thermodynamic changes in boreal extratropics have been attributed to human emissions with high confidence, but most circulation changes have not. In particular, not only that in the previous suite of climate models most models do not capture the recent boreal summer storm tracks weakening, but also a quantification of the role of human emissions in the recent storm tracks weakening has not been conducted to date. Here we use the latest suite of climate models, which are found to adequately capture the recent storm tracks weakening, and show that this weakening is attributable to anthropogenic emissions. Human emissions have resulted in more-rapid warming of the high latitudes, and the associated reduction in poleward temperature gradient has weakened the storms. The physical consistency between models and reanalyses increases our confidence in the projected weakening, which presents regional risks including hot-dry extremes in summer.
International perspectives on measuring national digital public health system maturity through a multidisciplinary Delphi study
Authors: Maaß Laura , Zeeb Hajo , Rothgang Heinz
Published: April 2024

Unlocking the full potential of digital public health (DiPH) systems requires a comprehensive tool to assess their maturity. While the World Health Organization and the International Telecommunication Union released a toolkit in 2012 covering various aspects of digitalizing national healthcare systems, a holistic maturity assessment tool has been lacking ever since. To bridge this gap, we conducted a pioneering Delphi study, to which 54 experts from diverse continents and academic fields actively contributed to at least one of three rounds. 54 experts participated in developing and rating multidisciplinary quality indicators to measure the maturity of national digital public health systems. Participants established consensus on these indicators with a threshold of 70% agreement on indicator importance. Eventually, 96 indicators were identified and agreed upon by experts. Notably, 48% of these indicators were found to align with existing validated tools, highlighting their relevance and reliability. However, further investigation is required to assess the suitability and applicability of all the suggestions put forward by our participants. Nevertheless, this Delphi study is an essential initial stride toward a comprehensive measurement tool for DiPH system maturity. By working towards a standardized assessment of DiPH system maturity, we aim to empower decision-makers to make informed choices, optimize resource allocation, and drive innovation in healthcare delivery. The results of this study mark a significant milestone in advancing DiPH on a global scale.
Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture – interaction studies based on a multi-OMICs approach
Authors: Kratzl Franziska , Urban Marlene , Pandhal Jagroop , Shi Mengxun , Meng Chen , Kleigrewe Karin , Kremling Andreas , Pflüger-Grau Katharina
Published: April 2024

In their natural habitats, microbes rarely exist in isolation; instead, they thrive in consortia, where various interactions occur. In this study, a defined synthetic co-culture of the cyanobacterium S. elongatus cscB, which supplies sucrose to the heterotrophic P. putida cscRABY, is investigated to identify potential interactions. Initial experiments reveal a remarkable growth-promoting effect of the heterotrophic partner on the cyanobacterium, resulting in an up to 80% increase in the growth rate and enhanced photosynthetic capacity. Vice versa, the presence of the cyanobacterium has a neutral effect on P. putida cscRABY, highlighting the resilience of pseudomonads against stress and their potential as co-culture partners. Next, a suitable reference process reinforcing the growth-promoting effect is established in a parallel photobioreactor system, which sets the basis for the analysis of the co-culture at the transcriptome, proteome, and metabolome levels. In addition to several moderate changes, including alterations in the metabolism and stress response in both microbes, this comprehensive multi-OMICs approach strongly hints towards the exchange of further molecules beyond the unidirectional feeding with sucrose. Taken together, these findings provide valuable insights into the complex dynamics between both co-culture partners, indicating multi-level interactions, which can be employed for further streamlining of the co-cultivation system.
An experimentally representative in-silico protocol for dynamical studies of lyophilised and weakly hydrated amorphous proteins
Authors: Bassotti Elisa , Gabrielli Sara , Paradossi Gaio , Chiessi Ester , Telling Mark
Published: April 2024

Characterization of biopolymers in both dry and weakly hydrated amorphous states has implications for the pharmaceutical industry since it provides understanding of the effect of lyophilisation on stability and biological activity. Atomistic Molecular Dynamics (MD) simulations probe structural and dynamical features related to system functionality. However, while simulations in homogenous aqueous environments are routine, dehydrated model assemblies are a challenge with systems investigated in-silico needing careful consideration; simulated systems potentially differing markedly despite seemingly negligible changes in procedure. Here we propose an in-silico protocol to model proteins in lyophilised and weakly hydrated amorphous states that is both more experimentally representative and routinely applicable. Since the outputs from MD align directly with those accessed by neutron scattering, the efficacy of the simulation protocol proposed is shown by validating against experimental neutron data for apoferritin and insulin. This work also highlights that without cooperative experimental and simulative data, development of simulative procedures using MD alone would prove most challenging.