bluuebunny/crossref_metadata_2025 huggingface dataset:
Mitochondria as novel mediators linking gut microbiota to atherosclerosis that is ameliorated by herbal medicine: A review
Authors: Li Yujuan , Yang Shengjie , Jin Xiao , Li Dan , Lu Jing , Wang Xinyue , Wu Min
Published: January 2023

Atherosclerosis (AS) is the main cause of cardiovascular disease (CVD) and is characterized by endothelial damage, lipid deposition, and chronic inflammation. Gut microbiota plays an important role in the occurrence and development of AS by regulating host metabolism and immunity. As human mitochondria evolved from primordial bacteria have homologous characteristics, they are attacked by microbial pathogens as target organelles, thus contributing to energy metabolism disorders, oxidative stress, and apoptosis. Therefore, mitochondria may be a key mediator of intestinal microbiota disorders and AS aggravation. Microbial metabolites, such as short-chain fatty acids, trimethylamine, hydrogen sulfide, and bile acids, also affect mitochondrial function, including mtDNA mutation, oxidative stress, and mitophagy, promoting low-grade inflammation. This further damages cellular homeostasis and the balance of innate immunity, aggravating AS. Herbal medicines and their monomers can effectively ameliorate the intestinal flora and their metabolites, improve mitochondrial function, and inhibit atherosclerotic plaques. This review focuses on the interaction between gut microbiota and mitochondria in AS and explores a therapeutic strategy for restoring mitochondrial function and intestinal microbiota disorders using herbal medicines, aiming to provide new insights for the prevention and treatment of AS.
Role of exosomes in hepatocellular carcinoma and the regulation of traditional Chinese medicine
Authors: Yao Man , Liang Shufang , Cheng Binbin
Published: January 2023

Hepatocellular carcinoma (HCC) usually occurs on the basis of chronic liver inflammatory diseases and cirrhosis. The liver microenvironment plays a vital role in the tumor initiation and progression. Exosomes, which are nanometer-sized membrane vesicles are secreted by a number of cell types. Exosomes carry multiple proteins, DNAs and various forms of RNA, and are mediators of cell-cell communication and regulate the tumor microenvironment. In the recent decade, many studies have demonstrated that exosomes are involved in the communication between HCC cells and the stromal cells, including endothelial cells, macrophages, hepatic stellate cells and the immune cells, and serve as a regulator in the tumor proliferation and metastasis, immune evasion and immunotherapy. In addition, exosomes can also be used for the diagnosis and treatment HCC. They can potentially serve as specific biomarkers for early diagnosis and drug delivery vehicles of HCC. Chinese herbal medicine, which is widely used in the prevention and treatment of HCC in China, may regulate the release of exosomes and exosomes-mediated intercellular communication. In this review, we summarized the latest progresses on the role of the exosomes in the initiation, progression and treatment of HCC and the potential value of Traditional Chinese medicine in exosomes-mediated biological behaviors of HCC.
Agrobacterium-mediated genetic transformation of the most widely cultivated superior clone Eucalyptus urophylla × Eucalyptus grandis DH32-29 in Southern China
Authors: Wang Xiaoping , Chen Shanshan , Zhang Haonan , Luo Ping , Zhou Fangping , Zeng Bingshan , Xu Jianmin , Fan Chunjie
Published: January 2023

Eucalyptus, as an economically important species for wood and paper industries, still remains a challenge to genetic improvement by transgenic technology owing to the deficiency of a highly efficient and stable genetic transformation system, especially in cultivated superior clones.Eucalyptus urophylla×Eucalyptus grandisclone DH32-29 is most widely planted in southern China, but it is relatively recalcitrant to adventitious bud regeneration, which blocks the establishment of a genetic transformation system. Here, an efficient adventitious bud regeneration and transformation system ofEucalyptuswas established usingE. urophylla×E. grandisDH32-29 as material. Thein vitroleaves from microshoots that were subcultured for 20–25 days were immersed into liquid Woody Plant Medium supplemented with 0.02 mg·L−1of α-naphthaleneacetic acid (NAA) and 0.24 mg·L−1of forchlorfenuron (callus-inducing medium (CIM)). After 15 days, explants were transferred to a medium containing 0.10 mg·L−1of NAA and 0.50 mg·L−1of 6-benzyladenine (shoot-inducing medium (SIM)) for adventitious bud induction. The highest regeneration efficiency of adventitious buds was 76.5%. Therefore, anAgrobacterium tumefaciens-mediated genetic transformation system was optimized. The leaves were precultured for 7 days and infected for 30 min withA. tumefaciensstrain EHA105 grown to a bacterial density of 0.3 (OD600). After 72 h of cocultivation in the dark, leaves were transferred to CIM supplemented with 100 mg·L−1of cefotaxime (Cef), 100 mg·L−1of timentin, and 15 mg·L−1of kanamycin (Kan) for 15 days to induce calluses. Then, the explants were transferred to SIM supplemented with the same concentration of antibiotics, and the fresh medium was replaced every 15 days until resistant adventitious buds appeared. After induction of roots in root-inducing medium supplemented with 200 mg·L−1of Cef and 75 mg·L−1of Kan, completely transgenic plants were obtained. With the use of the aforementioned method, the transformation frequency can reach 1.9%. This provides a powerful approach for genetic improvement ofE. urophylla×E. grandisDH32-29 and gene function analysis inEucalyptus.
Plant growth, salt removal capacity, and forage nutritive value of the annual euhalophyte Suaeda salsa irrigated with saline water
Authors: Wang Ning , Zhao Zhenyong , Zhang Xinyi , Liu Sihai , Zhang Ke , Hu Mingfang
Published: January 2023

Sustainable agricultural development in semiarid and arid regions is severely restricted by soil and water salinization. Cultivation of the representative halophyte Suaeda salsa, which can be irrigated with saline water and cultivated on saline soils, is considered to be a potential solution to the issues of freshwater scarcity, soil salinization, and fodder shortage. However, the salt removal capacity and differences in the forage nutritive value of S. salsa under different saline water treatments remain unknown. Using the methods of field trials and randomized blocks design, we quantified salt accumulation in the aboveground biomass, and the biochemical and nutritive value of field-cultivated S. salsa in arid northwestern China under irrigation with water of different salinities [i.e., freshwater or water containing10, 20, 30, or 40 g/L NaCl). The fresh and dry weights of S. salsa increased, then decreased, with increase in salinity. The salt content of the plant’s aboveground biomass increased to a constant range and, thus, the salt extraction of S. salsa was relatively stable under different salinities of irrigation water. Under the experimental conditions, the crude protein content significantly increased to 9.45% dry weight (DW) and then decreased to 6.85% DW, with an increase in salinity (p < 0.05). The neutral detergent fiber (42.93%–50.00% DW) and acid detergent fiber (34.76%–39.70% DW) contents were suitable for forage. The contents of trace elements, such as copper and zinc, were significantly increased after irrigation with saline water (p < 0.05). The forage of S. salsa is of high nutritive value for livestock, and contains low concentrations of anti-nutrients. Therefore, S. salsa can be considered for cultivation in saline soils irrigated with saline water. In addition, it provides a viable additional source of fodder in arid regions, where the availability of freshwater and non-saline arable land is limited.
Multidimensional analysis reveals environmental factors that affect community dynamics of arbuscular mycorrhizal fungi in poplar roots
Authors: Han Shuo , Wang Xia , Cheng Yao , Wu Guanqi , Dong Xiaoyi , He Xiangwei , Zhao Guozhu
Published: January 2023

Introduction

Poplar is a tree species with important production and application value. The symbiotic relationship between poplar and arbuscular mycorrhizal fungi (AMF) has a key role in ecosystem functioning. However, there remain questions concerning the seasonal dynamics of the AMF community in poplar roots, the relationship between AMF and the soil environment, and its ecological function.

Method

Poplar roots and rhizosphere soil were sampled at the end of April and the end of October. The responses of AMF communities to season, host age, and host species were investigated; the soil environmental factors driving community changes were analyzed.

Results

The diversity and species composition of the AMF community were higher in autumn than in spring. Season, host age, host species, and soil environmental factors affected the formation of the symbiotic mycorrhizal system and the AMF community. Differences in the communities could be explained by soil pH, total nitrogen, total phosphorus, total potassium, available potassium, and glomalin content.

Discussion

The AMF community was sensitive to changes in soil physicochemical properties caused by seasonal dynamics, particularly total potassium. The change in the mycorrhizal symbiotic system was closely related to the growth and development of poplar trees.
Assembly, annotation, and comparative analysis of Ipomoea chloroplast genomes provide insights into the parasitic characteristics of Cuscuta species
Authors: Wang Yu , Xu Jing , Hu Bin , Dong Chunxing , Sun Jin , Li Zixian , Ye Kangzhuo , Deng Fang , Wang Lulu , Aslam Mohammad , Lv Wenliang , Qin Yuan , Cheng Yan
Published: January 2023

In the Convolvulaceae family, around 1650 species belonging to 60 genera are widely distributed globally, mainly in the tropical and subtropical regions of America and Asia. Although a series of chloroplast genomes in Convolvulaceae were reported and investigated, the evolutionary and genetic relationships among the chloroplast genomes of the Convolvulaceae family have not been extensively elucidated till now. In this study, we first reported the complete chloroplast genome sequence of Ipomoea pes-caprae, a widely distributed coastal plant with medical values. The chloroplast genome of I. pes-caprae is 161667 bp in length, and the GC content is 37.56%. The chloroplastic DNA molecule of I. pes-caprae is a circular structure composed of LSC (large-single-copy), SSC (small-single-copy), and IR (inverted repeat) regions, with the size of the three regions being 88210 bp, 12117 bp, and 30670 bp, respectively. The chloroplast genome of I. pes-caprae contains 141 genes, and 35 SSRs are identified in the chloroplast genome. Our research results provide important genomic information for the molecular phylogeny of I. pes-caprae. The Phylogenetic analysis of 28 Convolvulaceae chloroplast genomes showed that the relationship of I. pes-caprae with I. involucrata or I. obscura was much closer than that with other Convolvulaccae species. Further comparative analyses between the Ipomoea species and Cuscuta species revealed the mechanism underlying the formation of parasitic characteristics of Cuscuta species from the perspective of the chloroplast genome.
Intracellular phosphate recycling systems for survival during phosphate starvation in plants
Authors: Yoshitake Yushi , Yoshimoto Kohki
Published: January 2023

Phosphorus (P) is an essential nutrient for plant growth and plants use inorganic phosphate (Pi) as their P source, but its bioavailable form, orthophosphate, is often limited in soils. Hence, plants have several mechanisms for adaptation to Pi starvation. One of the most common response strategies is “Pi recycling” in which catabolic enzymes degrade intracellular constituents, such as phosphoesters, nucleic acids and glycerophospholipids to salvage Pi. Recently, several other intracellular degradation systems have been discovered that salvage Pi from organelles. Also, one of sphingolipids has recently been identified as a degradation target for Pi recycling. So, in this mini-review we summarize the current state of knowledge, including research findings, about the targets and degradation processes for Pi recycling under Pi starvation, in order to further our knowledge of the whole mechanism of Pi recycling.
Heat stress tolerance in peas (Pisum sativum L.): Current status and way forward
Authors: Devi Jyoti , Sagar Vidya , Mishra Gyan P. , Jha Prakash Kumar , Gupta Nakul , Dubey Rakesh K. , Singh Prabhakar M. , Behera Tusar K. , Prasad P. V. Vara
Published: January 2023

In the era of climate change, the overall productivity of pea (Pisum sativum L.) is being threatened by several abiotic stresses including heat stress (HS). HS causes severe yield losses by adversely affecting several traits in peas. A reduction in pod yield has been reported from 11.1% to 17.5% when mean daily temperature increase from 1.4 to 2.2°C. High-temperature stress (30.5-33°C) especially during reproductive phase is known to drastically reduce both seed yield and germination. HS during germination and early vegetative stage resulted in poor emergence and stunted plant growth along with detrimental effects on physiological functions of the pea plant. To combat HS and continue its life cycle, plants use various defense strategies including heat escape, avoidance or tolerance mechanisms. Ironically, the threshold temperatures for pea plant and its responses are inconsistent and not yet clearly identified. Trait discovery through traditional breeding such as semi leaflessness (afila), upright growing habit, lodging tolerance, lower canopy temperature and small seeded nature has highlighted their utility for greater adaptation under HS in pea. Screening of crop gene pool and landraces for HS tolerance in a targeted environment is a simple approach to identify HS tolerant genotypes. Thus, precise phenotyping using modern phenomics tools could lead to increased breeding efficiency. The NGS (next generation sequencing) data can be associated to find the candidate genes responsible for the HS tolerance in pea. In addition, genomic selection, genome wide association studies (GWAS) and marker assisted selection (MAS) can be used for the development of HS tolerant pea genotypes. Additionally, development of transgenics could be an alternative strategy for the development of HS tolerant pea genotypes. This review comprehensively covers the various aspects of HS tolerance mechanisms in the pea plant, screening protocols, omic advances, and future challenges for the development of HS tolerant genotypes.
Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection
Authors: Tang Qianjun , Ma Qianqian , Xiao Zhipeng , Xiao Yansong , Wang Yunsheng , Liu Lei , Peng Weiye , Wang Bing , Liu Tianbo , Song Na
Published: January 2023

Tobacco target spot disease is caused by a ubiquitous soil-borne phytopathogen Rhizoctonia solani; the pathogenic mechanisms underlying the effects of R. solani remain unclear. Deeper understanding of the functional responses to R. solani during host plant infection would help identify the molecular mechanisms essential for successful host invasion. In this study, we performed global transcriptional analysis of R. solani during various stages (12, 24, 48, 72, 96, and 120 h) of tobacco infection via an RNA sequencing method, while utilizing the pathosystem model R. solani AG3–tobacco (Nicotiana tabacum L.). After R. solani inoculation, the number of differentially expressed genes of R. solani differed at the various time points. Moreover, several gene ontology and Kyoto encyclopedia of genes and genomes pathways were unique in different infection stages, especially with respect to the genes involved in plant cell wall degradation and catalysis of biotransformation reactions, such as the pectin metabolic process and pectin catabolic process. The overexpressing-PD8 N. benthamiana plants enhanced the susceptibility to R. solani. In addition, we found that large amounts of reactive oxygen species (ROS) were generated in tobacco after infected by R. solani. R. solani encoding FAD/NAD binding oxidoreductase and peroxidase gene family to eliminating ROS and counteract oxidative stress. Moreover, Perox3 was validated that can enhance the ability of scavenging ROS by co-injecting. Overall, our findings show that pectin-degrading enzymes and cytochrome P450 genes are critical for plant infection. These results provide comprehensive insights into R. solani AG3 transcriptome responses during tobacco invasion.
Evaluating how lodging affects maize yield estimation based on UAV observations
Authors: Liu Yuan , Nie Chenwei , Zhang Zhen , Wang ZiXu , Ming Bo , Xue Jun , Yang Hongye , Xu Honggen , Meng Lin , Cui Ningbo , Wu Wenbin , Jin Xiuliang
Published: January 2023

Timely and accurate pre-harvest estimates of maize yield are vital for agricultural management. Although many remote sensing approaches have been developed to estimate maize yields, few have been tested under lodging conditions. Thus, the feasibility of existing approaches under lodging conditions and the influence of lodging on maize yield estimates both remain unclear. To address this situation, this study develops a lodging index to quantify the degree of lodging. The index is based on RGB and multispectral images obtained from a low-altitude unmanned aerial vehicle and proves to be an important predictor variable in a random forest regression (RFR) model for accurately estimating maize yield after lodging. The results show that (1) the lodging index accurately describes the degree of lodging of each maize plot, (2) the yield-estimation model that incorporates the lodging index provides slightly more accurate yield estimates than without the lodging index at three important growth stages of maize (tasseling, milking, denting), and (3) the RFR model with lodging index applied at the denting (R5) stage yields the best performance of the three growth stages, with R2 = 0.859, a root mean square error (RMSE) of 1086.412 kg/ha, and a relative RMSE of 13.1%. This study thus provides valuable insight into the precise estimation of crop yield and demonstra\tes that incorporating a lodging stress-related variable into the model leads to accurate and robust estimates of crop grain yield.
LIP5, a MVB biogenesis regulator, is required for rice growth
Authors: Wang Mengxue , Luo Shuwei , Fan Baofang , Zhu Cheng , Chen Zhixiang
Published: January 2023

LYST-INTERACTING PROTEIN5 (LIP5) is a conserved regulator of multivesicular body (MVB) biogenesis in eukaryotes. In Arabidopsis, AtLIP5 is a target of stress-responsive MITOGEN-ACTIVATED PROTEIN KINASE3 and 6 and mediates stress-induced MVB biogenesis to promote stress responses. However, Arabidopsis atlip5 knockout mutants are normal in growth and development. Here we report that rice OsLIP5 gene could fully restore both the disease resistance and salt tolerance of the Arabidopsis oslip5 mutant plants to the wild-type levels. Unlike Arabidopsis atlip5 mutants, rice oslip5 mutants were severely stunted, developed necrotic lesions and all died before flowering. Unlike in Arabidopsis, LIP5 regulated endocytosis under both stress and normal conditions in rice. These findings indicate that there is strong evolutionary divergence among different plants in the role of the conserved LIP5-regulated MVB pathway in normal plant growth.
Early lexical processing of Chinese one-character words and Mongolian words: A comparative study using event-related potentials
Authors: Zhang Kai , Gu Feng , Yu Hongzhi
Published: January 2023

Logographic language and alphabetic language differ significantly in orthography. Investigating the commonality and particularity of visual word recognition between the two distinct writing systems is informative for understating the neural mechanisms underlying visual word recognition. In the present study, we compared the chronometry of early lexical processing and the brain regions involved in early lexical processing between Chinese (logographic language) and Mongolian (alphabetic language) by recording event-related potentials (ERPs) using both implicit and explicit reading tasks. Familiar Chinese one-character words (lexical) and unknown Chinese one-character words (non-lexical) were pseudorandomly presented to native Chinese readers in Experiment 1. Mongolian words (lexical) and pseudowords (non-lexical) were pseudorandomly presented to native Mongolian readers in Experiment 2. In the color decision task, participants were asked to decide the color (black or blue) of each stimulus. In the lexical recognition task, participants were asked to report whether they could recognize each stimulus. The results showed that in both experiments and both tasks, ERPs to lexical items differed significantly from those to non-lexical items in the parietooccipital scalp region approximately 250 ms after stimulus onset, reflecting the early lexical processing, which likely originated from the ventral occipitotemporal cortex as revealed by source analysis. These results indicated that although Chinese and Mongolian differed markedly in orthographic features, the neural mechanisms underlying early lexical processing are similar between the two languages.
Development and validation of a highly dynamic and reusable picture-based scale: A new affective measurement tool
Authors: Liu Ping , Wang Ya’nan , Hu Jiang’ning , Qing Lin’bo , Zhao Ke
Published: January 2023

Emotion measurement is crucial to conducting emotion research. Numerous studies have extensively employed textual scales for psychological and organizational behavior research. However, emotions are transient states of organisms with relatively short duration, some insurmountable limitations of textual scales have been reported, including low reliability for single measurement or susceptibility to learning effects for multiple repeated use. In the present article, we introduce the Highly Dynamic and Reusable Picture-based Scale (HDRPS), which was randomly generated based on 3,386 realistic, high-quality photographs that are divided into five categories (people, animals, plants, objects, and scenes). Affective ratings of the photographs were gathered from 14 experts and 209 professional judges. The HDRPS was validated using the Self-Assessment Manikin and the PANAS by 751 participants. With an accuracy of 89.73%, this new tool allows researchers to measure individual emotions continuously for their research. The non-commercial use of the HDRPS system can be freely accessible by request at http://syy.imagesoft.cc:8989/Pictures.7z. HDRPS is used for non-commercial academic research only. As some of the images are collected through the open network, it is difficult to trace the source, so please contact the author if there are any copyright issues.
The Impact of Team Learning Climate on Innovation Performance – Mediating role of knowledge integration capability
Authors: Li Ming-Shun , Li Jiaqi , Li Jia-Mei , Liu Zi-Wei , Deng Xin-Tao
Published: January 2023

To address the fierce competition for corporate innovation in the digital economy, this study introduces knowledge integration capability as a mediating variable in light of social information processing theory, and explores the mechanism of team learning climate on innovation performance. Data were collected from a sample of 184 team members for statistical analysis, and Statistical methods such as descriptive statistical analysis, correlation analysis, and regression analysis were used to verify the study hypotheses through SPSS and Amos software, and the results showed that: (1) Team learning climate has a significant positive effect on knowledge integration capability. (2) Team learning climate has a significant positive effect on innovation performance. (3) Knowledge integration capability has a significant positive effect on innovation performance. (4) Knowledge integration capability partially mediates the role between team learning climate and innovation performance. The results proved the perspective of knowledge integration capability for the mechanism of team learning climate on innovation performance from the perspective of knowledge integration capability, and provided theoretical references for creating a learning climate in companies to promote members’ knowledge learning and enhance innovation performance.
Effect of mask coverage on face identification in Taiwanese men and women
Authors: Chen Yi-Lang , Wu Cheng-Yu , Li Shih-Cheng , Yu Tai-Min , Yu Shu-Ping
Published: January 2023

Mask wearing is the easiest and most effective way to avoid COVID-19 infection; however, it affects interpersonal activities, especially face identification. This study examined the effects of three mask coverage levels (full coverage, FC; coverage up to the middle [MB] or bottom of the nose bridge [BB]) on face identification accuracy and time. A total of 115 university students (60 men and 55 women) were recruited to conduct a computer-based simulation test consisting of 30 questions (10 questions [five face images each of men and women] for the three mask coverage levels). One unmasked target face and four face images with a specified mask coverage level were designed for each question, and the participants were requested to select the same face from the four covered face images on the basis of the target face. The ANOVA results indicated that identification accuracy was significantly affected by sex (p < 0.01) and the mask coverage level (p < 0.001), whereas identification time was only influenced by sex (p < 0.05). The multiple comparison results indicated that the identification accuracy rate for faces wearing a mask with FC (90.3%) was significantly lower than for those wearing masks with coverage up to the MB (93.7%) and BB (94.9%) positions; however, no difference in identification accuracy rate was observed between the MB and BB levels. Women exhibited a higher identification accuracy rate than men (94.1% vs. 91.9%) in identifying unfamiliar faces, even though they may spend less time identifying the images. A smaller mask coverage level (i.e., the BB level) does not facilitate face identification. The findings can be served as a reference for people to trade-off between wearing a mask and interpersonal interaction in their daily activities.
Pre-operative administration of butorphanol mitigates emergence agitation in patients undergoing functional endoscopic sinus surgery: A randomized controlled clinical trial
Authors: Zhang Xiao , Qi Siyi , Lin Zhen , Zhang Yizhe , Dai Wanbing , Tian Weitian , Tian Jie , Zheng Li , Su Diansan , Huai Xiaorong
Published: January 2023

Background

This study explored the effectiveness of pre-operative intravenous injection of butorphanol in the alleviation of emergence agitation (EA) in patients undergoing functional endoscopic sinus surgery (FESS).

Methods

Patients (n = 708) were randomized into two groups. The butorphanol group (Group B, n = 358) received butorphanol infusion (20 ug/kg) before anesthesia induction, while the control group (Group C, n = 350) received an equal volume of normal saline infusion. General anesthesia was induced with sufentanil, propofol, and rocuronium, and was maintained with sevoflurane and remifentanil. Vasoactive drugs maintained the hemodynamic indices within 20% of the baseline.

Results

The incidence of EA was significantly lower in Group B than that in Group C (Group B vs. C: 24.3% vs. 31.4%, respectively; P = 0.034). The times to spontaneous breathing (26.5 min vs. 23.7 min, P = 0.011), verbal response (36.0 min vs. 33.4 min, P = 0.012), and extubation (31.0 min vs. 28.7 min, P = 0.025) were longer in Group B, and the grade of cough (0.33 vs. 0.43, P = 0.024) at extubation in Group B was lower than that in Group C (P = 0.024). The mean arterial pressure at the end of the operation (P = 0.004) and at 5 min after extubation (P = 0.008) was higher and hypotension was less prominent (0.6% vs. 2.6%, P = 0.030) in Group B.

Conclusion

Pre-operative intravenous injection of butorphanol decreased the incidence of EA after FESS and provided smooth and hemodynamically stable emergence without extending the stay in post-anesthesia care unit.

Clinical trial registration

https://www.clinicaltrials.gov/, identifier NCT03398759.
The link between diabetic retinal and renal microvasculopathy is associated with dyslipidemia and upregulated circulating level of cytokines
Authors: Chen Xiaosi , Zhang Xinyuan , Gong Zhizhong , Yang Yang , Zhang Xiaohong , Wang Qiyun , Wang Yanhong , Xie Rui
Published: January 2023

Purpose

To investigate the mechanisms underlying the correlations between diabetic retinopathy (DR) and diabetic nephropathy (DKD) and examine whether circulating cytokines and dyslipidemia contribute to both DR and DKD in patients with 2 diabetes mellitus (T2DM).

Methods

A total of 122 patients with T2DM were enrolled and categorized into the DM group (without no DR and DKD), DR group [non-proliferative DR (NPDR), and proliferative DR (PDR)] with no DKD), DR complicated with DKD groups (DR+DKD group). The biochemical profile, including fasting blood glucose (FBG), glycated hemoglobin (HbA1c), and lipid profile were estimated, and plasma inflammatory and angiogenic cytokines [monocyte chemoattractant protein-1 (MCP-1), interleukin (IL)-6, IL-8, vascular endothelial growth factor (VEGF)-A, C, D, and placental growth factor (PlGF)] were analyzed by protein microarrays. The atherogenic plasma index (API) was defined as low-density lipoprotein cholesterol (LDL-C)/high-density lipoprotein-cholesterol (HDL-C); atherogenic index (AI) was calculated as [(total cholesterol (TC) -HDL-C)/HDL-C], and atherogenic index of plasma (AIP) was defined as log (TG/HDL-C).

Results

By multivariable disordered regression analysis, after controlling for duration of DM and hypertension, LDL-C (p = 0.019) and VEGF-D (p = 0.029) resulted as independent risk factors for DR. Albumin-to-creatinine ratio (uACR) (p = 0.003) was an independent risk factor for DR with DKD. In DR, NPDR, and PDR groups, grades of A1, A2, and A3 of albuminuria increased with the severity of DR. In A1, A2, and A3 grade groups, the severity of DR (DM, NPDR, and PDR) increased with higher albuminuria grades. Kendall's tau-b correlation coefficient analysis revealed that FBG (p = 0.019), circulating level of PlGF (p = 0.002), and VEGF-D (p = 0.008) were significantly positively correlated with the grades of uACR (p < 0.001), and uACR grades were significantly correlated with DR severity (p < 0.001).

Conclusions

The occurrence and severity of DR are closely correlated with kidney dysfunction. Among the three kidney functional parameters, uACR resulted as the better indicator of DR severity and progression than glomerular filtration (eGFR) and serum creatinine (Scr). Impaired FBG was associated with microalbuminuria, emphasizing that well-controlled FBG is important for both DR and DKD. The link between diabetic retinal and renal microvasculopathy was associated with dyslipidemia and upregulated circulating level of angiogenic cytokines.
Sexual behaviors, cannabis, alcohol and monkeypox infection
Authors: Vallée Alexandre
Published: January 2023

The emergence of the monkeypox virus (MPXV) outbreak in 2022 is a worldwide health issue. The rapid increase of monkeypox cases caused the WHO to designate the escalating global monkeypox outbreak a Public Health Emergency of International Concern on July 23, 2022. The WHO has called on the group currently most affected by the virus, men who have sex with men (MSM), to limit their sexual partners. The diminution in number of sexual partners not only decreases the proportion of infected MSM but could also increases the number of days needed to reach a given infection level among the general population. Several behavioral factors could be associated with high levels of different sexual partners, such as cannabis use and alcohol consumption. Firstly, this review focuses on the association between cannabis and alcohol consumption and the number of sexual partners, and their possible impact on the current MPXV outbreak by impairing the immune responses. Secondly, this review investigated in the UK Biobank cohort the relationship between alcohol and cannabis use and the number of sexual partners. Among the 115,604 participants, 1.8% declared to be MSM, 1.9% to be WSW (women having sex with women), 43.3% men heterosexuals and 53.0% women heterosexuals. MSM and WSW showed higher lifetime sexual partners (N = 17.4 (SD:17.52) and N = 13.65 (SD: 13.21), respectively) compared to heterosexual men (N = 6.89 (SD: 9.47) and women (N = 5.19 (SD:6.56), p < 0.001. After adjustment for age, body mass index, lifetime sexual activity, educational and income levels, tobacco and cardiovascular diseases, cannabis use and alcohol consumption remained significantly associated with increase in the number of different sexual partners in all four subgroups. Thus, cannabis use and alcohol consumption may have two detrimental effects on the MPXV outbreak: by participating in the increase of the number of sexual partners which are mainly responsible for the augmentation of the number of new MPXV infected cases and by impairing the immune response to a viral infection. Health and safety policies should address the factors and practices, including chemsex, leading to an increase in risk of sexual behaviors responsible for MPXV dissemination in the worldwide population.
Co-creation in a digital health living lab: A case study
Authors: Fotis Theofanis , Kioskli Kitty , Sundaralingam Anand , Fasihi Amer , Mouratidis Haralambos
Published: January 2023

Co-creation in healthcare, especially in developing digital health solutions, has been widely identified as a fundamental principle for person-centered technologies that could accelerate the adaptation of innovation. A Digital Health Living Lab based on community offers a sustainable and real-life environment to ideate, develop, and evaluate digital health solutions addressing the needs of multiple stakeholders. This article presents the experience of the School of Sport and Health Sciences at the University of Brighton in establishing a Digital Health Living Lab. In addition, we share a proposed step-by-step approach to establishing such a living lab in the community, supplemented by a case study of product development.
Methods for eliciting and measuring behavioral and physiological consequences of stress and uncertainty in virtual reality
Authors: Brunyé Tad T. , Giles Grace E.
Published: January 2023

Military operations are characterized by high levels of stress and uncertainty, and these states can influence cognitive and physical performance outcomes. These states, however, can be difficult to reliably induce in laboratory contexts, making it challenging to quantify and model their influences on perceptual and cognitive processes underlying performance on applied tasks. Herein we describe the development and validation of a novel scenario-based virtual reality methodology, the decision making under uncertainty and stress (DeMUS) scenario, that accomplishes four primary goals. First, it induces physiological and biochemical stress responses through a threat of shock manipulation. Second, it induces transient states of uncertainty by manipulating stimulus clarity in a perceptual decision-making task. Third, it generates several performance metrics regarding recognition memory, spatial orienting, threat classification, and marksmanship decision making. Finally, the task combines behavioral, physiological, and biochemical measures to provide a more comprehensive understanding of how stress and uncertainty influence applied task performance. To provide an initial validation of the scenario and its associated tasks and measures, we conducted a pilot study (n= 18) involving stress induction and cognitive performance assessment. Analyses revealed that: 1) the DeMUS scenario elicited tonic and phasic biochemical (salivary alpha amylase and cortisol) and physiological (heart rate, pupil diameter) stress responses, 2) the scenario elicited variable sympathetic autonomic nervous system and hypothalamic-pituitary adrenal (HPA) axis responses, and 3) stress influenced some measures of memory and decision-making in both negative and positive directions. Continuing research will assess individual- and group-level predictors of performance on these virtual reality tasks, and emerging performance enhancement techniques that can help military personnel sustain performance during stressful operations.
The first case report of an intraosseous epidermoid cyst in the distal phalanx of the index finger with infection resulting in single clubbing finger: A case report and review of the literature
Authors: Alhaskawi Ahmad , Zhou Haiying , Dong Yanzhao , Lai Jingtian , Wang Zewei , Hasan Abdullah Ezzi Sohaib , Goutham Kota Vishnu , Hasan Abdulla Hasan Abdulla Mohamed , Sun Zhenyu , Lu Hui
Published: January 2023

An intraosseous epidermoid cyst at the distal phalanx of the index finger is extremely rare. These cysts are asymptomatic unless ruptured, severely infected, or transformed into malignant squamous cell carcinoma. We present a case of a single clubbing finger in an adult diagnosed with an intraosseous epidermoid cyst in the distal phalanx of the left index finger with no history of pulmonary or cardiovascular diseases. Preoperative MRI showed an expansile lytic lesion with a sclerotic margin. Histopathological examination indicates that there is keratinous cell debris in the cyst with a wall of stratified squamous epithelium, which was the key to the correct diagnosis of an intraosseous epidermoid cyst. Written informed consent was obtained from the patient for publication of this case report and any accompanying images.
The impact of SARS-CoV-2 measures on patient samples and complication rates in spine surgery – A single center analysis
Authors: Jankovic Dragan , Krenzlin Harald , Keric Naureen , Ottenhausen Malte
Published: January 2023

Objective

Over the past two years during the pandemic, the German health system has taken drastic measures, like the continuous use of face masks for all staff, restrictions of hospital visits as well as cancellation of elective surgical procedures. As a potential side effect of these measures, a significant reduction of surgical site infections was reported for neurosurgical patients. The purpose of our study was to analyze the impact of these measures on spinal surgery.

Methods

We performed a retrospective analysis to compare patient samples, procedures and infection rates before (January 2019 – March 2020) and during (April 2020 – June 2021) the pandemic to evaluate the impact of the measures mentioned above. Demographic and clinical data were collected and correlated with the occurrence of postoperative complications, especially infection.

Results

Our analysis showed no relevant decrease of spine surgeries (838 surgeries in non-pandemic group vs. 831 surgeries pandemic group). The most common postoperative complication was wound infection in both groups, followed by urinary tract infection and pneumonia. In both patient groups, infections were more prevalent in surgeries of multilevel posterior instrumentation. Comparing the two groups of patients, a slight, non-significant (0.5%) reduction of overall postoperative complications in the pandemic group was observed. However, the number of spinal surgeries classified as emergencies in our institution increased by 10.2% during the last 15 months of the COVID-19 pandemic. In line with this finding the urgent transfer of patients from smaller hospitals increased by 14.2%, compared to previous years.

Conclusion

The volume of spinal surgeries remained high and complication rates stable during the pandemic. A reason why complication rates did not drop as reported previously might be a significant change in patient sample due to the increase of emergency surgeries. A decrease of complication rates, especially infections by the measures of infection prevention for the pandemic was not observed.
Noxious effects of riot control agents on the ocular surface: Pathogenic mechanisms and management
Authors: Quiroga-Garza Manuel E. , Ruiz-Lozano Raul E. , Azar Nadim S. , Mousa Hazem M. , Komai Seitaro , Sevilla-Llorca Jose L. , Perez Victor L.
Published: January 2023

Riot Control Agents (RCAs) are chemical compounds used by law enforcement agencies to quell violent demonstrations as an alternative to lethal force and as part of police/military training. They are also known as tear gases because of the hallmark ocular irritation and lacrimation they cause. The most common RCAs include oleoresin capsicum (contained in Mace and pepper spray), chlorobenzylidene malononitrile, dibenzoxazepine, and chloroacetophenone (previously the main content of Mace); some of which have been in use for decades. Their immediate incapacitating effects are mediated through polymodal afferent fibers innervating the corneal surface, inducing the release of peptides that cause neurogenic inflammation. Although previously thought to have only transient effects on exposed patients more severe complications such as corneal stromal opacities, corneal neovascularization, neurotrophic keratopathy, conjunctival necrosis, and pseudopterygium can occur. Concerningly, the lack of research and specific therapies restrict the current management to decontamination and symptom-tailored support. This manuscript will provide an overview of the toxic mechanisms of RCAs, their clinical manifestations, and current therapy after exposure to tear gases.
Burkholderia pseudomallei in soil and natural water bodies in rural Sri Lanka: A hidden threat to public health
Authors: Jayasinghearachchi Himali S. , Muthugama Thilini A. , Masakorala Jayanthi , Kulasekara Upeksha S. , Jayaratne Kumari , Jayatunga D. A. Dasun N. , De Silva Aruna D. , Corea Enoka M.
Published: January 2023

Burkholderia pseudomallei is the causative agent of the potentially fatal infection, melioidosis. This study provides the first evidence for the presence of B. pseudomallei in soil and water in Sri Lanka. Targeted sampling of soil and natural water sources was done between November 2019 and October 2020 over eight field visits encompassing the neighborhood of 28 culture and/or antibody-positive melioidosis patients in northwestern, western and southern Sri Lanka. A total of eight environmental isolates of B. pseudomallei (BPs-env1 to BPs-env8) were cultured from 116 soil and 117 natural water samples collected from 72 locations. The presence of B. pseudomallei in soil and natural water in these areas poses a risk of melioidosis for populations cultivating crops in such soils and using untreated water from these sources for drinking, bathing, and other domestic purposes. Identifying sites positive for B. pseudomallei may help to mitigate risk by raising public awareness of contaminated environmental sources and allowing soil and water remediation.
Identification of mutations in porcine STAT5A that contributes to the transcription of CISH
Authors: Yao Diwen , Guo Dongchun , Zhang Yingkun , Chen Zhihua , Gao Xiaowen , Xing Guiling , Yang Xiuqin , Wang Xibiao , Di Shengwei , Cai Jiancheng , Niu Buyue
Published: January 2023

Identification of causative genes or genetic variants associated with phenotype traits benefits the genetic improvement of animals. CISH plays a role in immunity and growth, however, the upstream transcriptional factors of porcine CISH and the genetic variations in these factors remain unclear. In this study, we firstly identified the minimal core promoter of porcine CISH and confirmed the existence of STATx binding sites. Overexpression and RT-qPCR demonstrated STAT5A increased CISH transcriptional activity (P < 0.01) and mRNA expression (P < 0.01), while GATA1 inhibited CISH transcriptional activity (P < 0.01) and the following mRNA expression (P < 0.05 or P < 0.01). Then, the putative functional genetic variations of porcine STAT5A were screened and a PCR-SSCP was established for genotype g.508A>C and g.566C>T. Population genetic analysis showed the A allele frequency of g.508A>C and C allele frequency of g.566C>T was 0.61 and 0.94 in Min pigs, respectively, while these two alleles were fixed in the Landrace population. Statistical analysis showed that Min piglets with CC genotype at g.566C>T or Hap1: AC had higher 28-day body weight, 35-day body weight, and ADG than TC or Hap3: CT animals (P < 0.05, P < 0.05). Further luciferase activity assay demonstrated that the activity of g.508A>C in the C allele was lower than the A allele (P < 0.05). Collectively, the present study demonstrated that STAT5A positively regulated porcine CISH transcription, and SNP g.566C>T in the STAT5A was associated with the Min piglet growth trait.
Iterative Image Reconstruction Algorithm with Parameter Estimation by Neural Network for Computed Tomography
Authors: Kojima Takeshi , Yoshinaga Tetsuya
Published: January 2023

Recently, an extended family of power-divergence measures with two parameters was proposed together with an iterative reconstruction algorithm based on minimization of the divergence measure as an objective function of the reconstructed images for computed tomography. Numerical experiments on the reconstruction algorithm illustrated that it has advantages over conventional iterative methods from noisy measured projections by setting appropriate values of the parameters. In this paper, we present a novel neural network architecture for determining the most appropriate parameters depending on the noise level of the projections and the shape of the target image. Through experiments, we show that the algorithm of the architecture, which has an optimization sub-network with multiplicative connections rather than additive ones, works well.
A Real-Time Novelty Recognition Framework Based on Machine Learning for Fault Detection
Authors: Albertin Umberto , Pedone Giuseppe , Brossa Matilde , Squillero Giovanni , Chiaberge Marcello
Published: January 2023

New technologies are developed inside today’s companies with the ascent of Industry 4.0 paradigm; Artificial Intelligence applied to Predictive Maintenance is one of these, helping factories automate their systems in detecting anomalies. The deviation of statistical features from standard operating conditions computed on collected data is a common investigation technique that companies use. The information loss due to transformation from raw data to extracted features is a problem of this approach. Furthermore, a common Predictive Maintenance framework requires historical data about failures that often do not exist, neglecting the possibility of applying it. This paper uses Artificial Intelligence as Machine Learning models to recognize when something changes in the data’s behavior collected up to that moment, also helping companies to gather a preliminary dataset for future Predictive Maintenance implementation. The aim concerns a framework in which several sensors are used to collect data by adopting a sensor fusion approach. The architecture is composed of an optimized software system able to enhance the computation scalability and the response time regarding novelty detection. This article analyzes the proposed architecture, then explains a proof-of-concept development using a digital model; finally, two real cases are studied to show how the framework behaves in a real environment. The analysis done in this paper has an application-oriented approach; hence a company can directly use the framework in its systems.