bluuebunny/crossref_metadata_2025 huggingface dataset:
On the Dependence of the Critical Success Index (CSI) on Prevalence
Authors: Mbizvo Gashirai K. , Larner Andrew J.
Published: March 2024

The critical success index (CSI) is an established metric used in meteorology to verify the accuracy of weather forecasts. It is defined as the ratio of hits to the sum of hits, false alarms, and misses. Translationally, CSI has gained popularity as a unitary outcome measure in various clinical situations where large numbers of true negatives may influence the interpretation of other, more traditional, outcome measures, such as specificity (Spec) and negative predictive value (NPV), or when unified interpretation of positive predictive value (PPV) and sensitivity (Sens) is needed. The derivation of CSI from measures including PPV has prompted questions as to whether and how CSI values may vary with disease prevalence (P), just as PPV estimates are dependent on P, and hence whether CSI values are generalizable between studies with differing prevalences. As no detailed study of the relation of CSI to prevalence has been undertaken hitherto, the dataset of a previously published test accuracy study of a cognitive screening instrument was interrogated to address this question. Three different methods were used to examine the change in CSI across a range of prevalences, using both the Bayes formula and equations directly relating CSI to Sens, PPV, P, and the test threshold (Q). These approaches showed that, as expected, CSI does vary with prevalence, but the dependence differs according to the method of calculation that is adopted. Bayesian rescaling of both Sens and PPV generates a concave curve, suggesting that CSI will be maximal at a particular prevalence, which may vary according to the particular dataset.
Enterobacterales Biofilm-Specific Genes and Antimicrobial and Anti-Inflammatory Biomarkers in the Blood of Patients with Ischemic Heart Disease
Authors: Giedraitiene Agne , Tatarunas Vacis , Kaminskaite Kornelija , Meskauskaite Ugne , Boieva Svitlana , Ajima Yu , Ciapiene Ieva , Veikutiene Audrone , Zvikas Vaidotas , Kupstyte-Kristapone Nora , Jakstas Valdas , Luksiene Dalia , Tamosiunas Abdonas , Lesauskaite Vaiva
Published: March 2024

Background: Ischemic heart disease (IHD) is the most prevalent type of cardiovascular disease. The main cause of IHD is atherosclerosis, which is a multifactorial inflammatory disease of blood vessels. Studies show that bacteria might have a significant impact on the pathogenesis of atherosclerosis and plaque rupture. This study aimed to evaluate the complexity of interactions between bacteria and the human body concerning metabolites and bacterial genes in patients with ischemic heart disease. Methods: Bacterial 16S rDNA and wcaF, papC, and sdhC genes were detected in whole blood using a real-time PCR methodology. An enzyme-linked immunosorbent assay was used to measure the concentration of the LL-37 protein. An analysis of ARA in blood plasma was performed. Results: Bacterial 16S rDNA was detected in 31% of the study patients, and the genes wcaF and sdhC in 20%. Enterobacterales genes were detected more frequently in patients younger than 65 years than in patients aged 65 years and older (p = 0.018) and in patients with type 2 diabetes (p = 0.048). Concentrations of the human antimicrobial peptide LL-37 and 12S-HETE concentrations were determined to be higher if patients had 16S rDNA and biofilm-specific genes. Conclusions: The results of this study enhance the understanding that Enterobacterales bacteria may participate in the pathogenesis of atherosclerosis and IHD. Bacterial DNA and host metabolites in higher concentrations appear to be detected.
Fetal Left Ventricle Function Evaluated by Two-Dimensional Speckle-Tracking Echocardiography across Clinical Stages of Severity in Growth-Restricted Fetuses
Authors: Domínguez-Gallardo Carla , Ginjaume-García Nuria , Ullmo Johana , Parra Juan , Vázquez Ana , Cruz-Lemini Mónica , Llurba Elisa
Published: March 2024

Fetal growth restriction (FGR) can result in adverse perinatal outcomes due to cardiac dysfunction. This study used 2D speckle-tracking echocardiography to assess left ventricle (LV) longitudinal strain across FGR severity stages. A prospective longitudinal cohort study measured global (GLS) and segmental LV longitudinal strain in FGR fetuses, with evaluations conducted at various time points. FGR was classified into subtypes based on published criteria using fetal weight centile and Doppler parameters. A linear mixed model was employed to analyze repeated measures and compare Z-score measurements between groups throughout gestational age. The study included 40 FGR fetuses and a total of 107 evaluations were performed: 21 from small for gestational age (SGA), 74 from the FGR stage I, and 12 from the FGR stage ≥ II. The results indicate that SGA and stage I FGR fetuses exhibit higher LV GLS than stages ≥ II. Throughout gestation, SGA and FGR stage I fetuses showed similar behavior with consistently better LV GLS values when compared to FGR stages ≥ II. No significant differences were observed in LV GLS strain behavior between SGA and FGR stage I. In conclusion, all FGRs show signs of early cardiac dysfunction, with severe cases demonstrating significantly a lower LV GLS when compared to mild cases, suggesting deterioration of cardiac dysfunction with progression of fetal compromise.
Visualization Methods for Uterine Sentinel Lymph Nodes in Early-Stage Endometrial Carcinoma: A Comparative Analysis
Authors: Andreika Linas , Vankevičienė Karolina , Ramašauskaitė Diana , Rudaitis Vilius
Published: March 2024

Background: Sentinel lymph node (SLN) biopsy in early-stage endometrial cancer is recommended over systematic lymphadenectomy due to reduced morbidity and comparable detection rates. The main objective of this study was to compare the overall and bilateral detection rates of SLN in early-stage endometrial cancer using three techniques. Methods: a prospective cohort study was designed to detect the difference in SLN detection rate in three cohorts: Indocyanine green (ICG), methylene blue (MB), and tracer combination (ICG + MB). Mapping characteristics, detection rate, number of SLNs, and positive SLNs of the three cohorts were compared. Results: A total of 99 patients were enrolled. A total of 109 SLN sites with 164 lymph nodes were detected. No differences were found between the three cohorts in terms of age, BMI, tumor diameter, or other histologic characteristics. The overall SLN detection rate (DR) was 54.3% in the MB group, 72.7% in ICG, and 80.6% in the ICG-MB group. Bilateral DR was 22.9%, 39.4%, and 54.8% in groups, respectively, with the MB method yielding significantly inferior results. Conclusions: The ICG-MB group demonstrated superior overall and bilateral detection rates, but a significant difference was found only in the MB cohort. Combining tracer agents can enhance the accuracy of SLN identification in initial-stage endometrial cancer without additional risk to the patient.
Editorial for the Special Issue “Skin and Cutaneous Adnexal Tumors: Diagnosis and Management”
Authors: Koumaki Dimitra
Published: March 2024

We are delighted to present the Special Issue on “Skin and Cutaneous Adnexal Tumors: Diagnosis and Management” in Diagnostics [...]
Steel Surface Defect Detection Algorithm Based on YOLOv8
Authors: Song Xuan , Cao Shuzhen , Zhang Jingwei , Hou Zhenguo
Published: March 2024

To improve the accuracy of steel surface defect detection, an improved model of multi-directional optimization based on the YOLOv8 algorithm was proposed in this study. First, we innovate the CSP Bottleneck with the two convolutions (C2F) module in YOLOv8 by introducing deformable convolution (DCN) technology to enhance the learning and expression ability of complex texture and irregular shape defect features. Secondly, the advanced Bidirectional Feature Pyramid Network (BiFPN) structure is adopted to realize the weight distribution learning of input features of different scales in the feature fusion stage, allowing for more effective integration of multi-level feature information. Next, the BiFormer attention mechanism is embedded in the backbone network, allowing the model to adaptively allocate attention based on target features, such as flexibly and efficiently skipping non-critical areas, and focusing on identifying potentially defective parts. Finally, we adjusted the loss function from Complete-Intersection over Union (CIoU) to Wise-IoUv3 (WIoUv3) and used its dynamic non-monotony focusing property to effectively solve the problem of overfitting the low quality target bounding box. The experimental results show that the mean Average Precision (mAP) of the improved model in the task of steel surface defect detection reaches 84.8%, which depicts a significant improvement of 6.9% compared with the original YOLO8 model. The improved model can quickly and accurately locate and classify all kinds of steel surface defects in practical applications and meet the needs of steel defect detection in industrial production.
Impact of DC-DC Converters on the Energy Performance of a Dense Concentrator PV Array under Nonuniform Irradiance and Temperature Profiles
Authors: Fernández Álvaro , Rosell-Mirmi Joana , Regany Desideri , Vilarrubí Montse , Barrau Jérôme , Ibañez Manel , Rosell-Urrutia Joan
Published: March 2024

Efficiency losses resulting from electrical mismatching in densely packed photovoltaic arrays present a significant challenge, particularly exacerbated in nonuniformly illuminated receivers and under varying temperatures. Serial configurations are particularly susceptible to radiation nonuniformities, while parallel systems are negatively affected by temperature variations. Various authors have recommended the incorporation of electrical voltage and current sources to mitigate these losses. This study explores different electrical connection configurations utilizing concentrated photovoltaic (CPV) cells and DC-DC electrical current converters. A self-adaptive microfluidic cell matrix cooling system is employed to mitigate thermal dispersion caused by the highly nonuniform illumination profile. The obtained results for each configuration are compared with the total electrical power produced by individual cells, operating under identical radiation and temperature conditions to those of the entire array. The results reveal a noteworthy increase in production across all studied configurations, with the parallel–series arrangement demonstrating the most promising practical utility. This configuration exhibited a remarkable 50.75% increase in power production compared with the standard series connection.
A Prototype Reactor Promoting the Hg(0) Capture in the Simulated Flue Gas from Small-Scale Boilers by Using Copper Oxide- and Copper Sulfide-Coated Teflon Pipes
Authors: Deng Yinyou , Górecki Jerzy , Szramowiat-Sala Katarzyna , Macherzynski Mariusz
Published: March 2024

In this study, we designed a prototype reactor, the multiple pipes reactor (MPR), for Hg(0) capture, which can be applied in small-scale boilers. It was tested on a laboratory scale by comparing it with a fixed-bed type, the vertical glass reactor (VGR). In total, 200 mg of CuO and CuS was applied as sorbent materials to reduce the concentration of Hg(0) from the simulated flue gas, in both VGR and MPR reactors. The mercury capture measurements were performed in the same laboratory system at 125 °C and a flow rate of 54 L/h. The contact time between the sorbents and simulated flue gas in the VGR was 0.035 s for both materials. In the case of the MPR, it was 0.44 s (CuO coating) and 0.63 s (CuS coating), depending on the coating area. The contact area inside the VGR was 5.31 cm2, contrasting with the values of 13.19 cm2 (CuO coating) and 18.84 cm2 (CuS coating) in the MPRs. The average Hg(0) capture effectiveness of CuO (granulate) and CuS (granulate) was 51% and 67% in VGR, respectively. The MPR with CuO- and CuS-coating Teflon (PTFE) pipes promoted an average Hg(0) capture effectiveness reaching 65 (by 268%) and 94% (by 158%), respectively.
Harnessing Nanomaterials for Enhanced Energy Efficiency in Transpired Solar Collectors: A Review of Their Integration in Phase-Change Materials
Authors: Croitoru Cristiana , Bode Florin , Calotă Răzvan , Berville Charles , Georgescu Matei
Published: March 2024

The building sector plays an important role in the global climate change mitigation objectives. The reduction of CO2 emissions and energy consumption in the building sector has been intensively investigated in the last decades, with solar thermal energy considered to be one of the most promising solutions due to its abundance and accessibility. However, the discontinuity of solar energy has led to the study of thermal energy storage to improve the thermal performance of solar thermal systems. In this review paper, the integration of various types of phase-change materials (PCMs) in transpired solar collectors (TSC) is reviewed and discussed, with an emphasis on heat transfer enhancements, including nanomaterials. Thermal energy storage applied to TSC is studied in terms of design criteria, materials technologies, and its impact on thermal conductivity. This review highlights the potential of nanomaterial technology integration in terms of thermal performance improvements. The utilization of nanomaterials in solar walls holds the potential to significantly enhance their performance. The integration of diverse materials such as graphene, graphite, metal oxides, and carbon nanoparticles can pave the way for improving thermal conductivity.
External Multi-Gap Lightning Arrester Modeling Using the Integration Method
Authors: Rodríguez-Serna Johnatan M. , Villa-Acevedo Walter M. , López-Lezama Jesús M.
Published: March 2024

Electric power distribution networks are exposed to both internal and external disturbances. Lightning strikes are among the latter and are responsible for a significant percentage of damage in distribution transformers, especially in rural areas. Electric utilities must pay special attention to prevent damage and service interruption due to these unforeseeable events. In this context, Surge Protection Devices (SPDs) combined with a series of external air gaps are designed to safeguard electric equipment and systems from transient over-voltages. There are several well-known models of SPDs in the specialized literature; nonetheless, few studies have been carried out with external gaps and multi-gaps. The main contribution of this paper is a methodology to model the disruptive effect in an external air gap by determining the parameters of Kind’s and Chowdhuri’s models using the integration method. The adjustment of the model parameters is carried out by a genetic algorithm (GA). The proposed model was tested and validated using experimental measurements, and its capability to predict the time-to-breakdown under different impulse voltages was verified.
Comparative Technical and Economic Analyses of Hydrogen-Based Steel and Power Sectors
Authors: Alikulov Khusniddin , Aminov Zarif , Anh La Hoang , Xuan Tran Dang , Kim Wookyung
Published: March 2024

Decarbonizing the current steel and power sectors through the development of the hydrogen direct-reduction iron ore–electric arc furnace route and the 100% hydrogen-fired gas turbine cycle is crucial. The current study focuses on three clusters of research works. The first cluster covers the investigation of the mass and energy balance of the route and the subsequent application of these values in experiments to optimize the reduction yield of iron ore. In the second cluster, the existing gas turbine unit was selected for the complete replacement of natural gas with hydrogen and for finding the most optimal mass and energy balance in the cycle through an Aspen HYSYS model. In addition, the chemical kinetics in the hydrogen combustion process were simulated using Ansys Chemkin Pro to research the emissions. In the last cluster, a comparative economic analysis was conducted to identify the levelized cost of production of the route and the levelized cost of electricity of the cycle. The findings in the economic analysis provided good insight into the details of the capital and operational expenditures of each industrial sector in understanding the impact of each kg of hydrogen consumed in the plants. These findings provide a good basis for future research on reducing the cost of hydrogen-based steel and power sectors. Moreover, the outcomes of this study can also assist ongoing, large-scale hydrogen and ammonia projects in Uzbekistan in terms of designing novel hydrogen-based industries with cost-effective solutions.
Characteristics and Changes in the Properties of Cereal and Rapeseed Straw Used as Energy Feedstock
Authors: Stolarski Mariusz Jerzy , Welenc Michał , Krzyżaniak Michał , Olba-Zięty Ewelina , Stolarski Jakub , Wierzbicki Sławomir
Published: March 2024

Solid biofuels, including straw as production residue, are still the largest energy feedstock in the structure of primary energy production from renewable energy sources. However, the properties of straw as a solid biofuel can vary depending on the species from which it was produced and the harvest period and year. Therefore, this study aimed to assess the thermophysical properties and elemental composition of six types of straw (rye, oat, triticale, wheat, corn, and rapeseed straw) obtained over three consecutive years (2020, 2021, 2022). Rye straw had the lowest moisture (mean: 10.55%), ash (mean: 2.71% DM), nitrogen (mean: 0.54% DM) and chlorine (mean: 0.046% DM) contents and the highest carbon content (mean: 47.93% DM), a higher heating value—HHV (mean: 19.03 GJ Mg−1 DM) and a lower heating value—LHV (mean: 15.71 GJ Mg−1). Triticale straw had similar properties, classifying it into the same cluster as rye straw. Corn straw had a remarkably high moisture content (mean: 48.91%), low LHV and high chlorine content. Rapeseed straw contained high levels of Cl, S, N and ash, and they were 643%, 481%, 104% and 169% higher, respectively, than those in rye straw. The sulfur, chlorine and moisture contents of the six straw types under study were highly variable during the three years of the study. Knowledge of the properties of different types of straw as energy feedstocks facilitates the logistics and organization of the supply of bioenergy installations. However, further research is needed, especially studies assessing the energy intensity and logistical costs of different types of straw used for energy purposes.
Power Consumption Analysis of a Prototype Lightweight Autonomous Electric Cargo Robot in Agricultural Field Operation Scenarios
Authors: Loukatos Dimitrios , Arapostathis Vasileios , Karavas Christos-Spyridon , Arvanitis Konstantinos G. , Papadakis George
Published: March 2024

The continuous growth of the urban electric vehicles market and the rapid progress of the electronics industry create positive prospects towards fostering the development of autonomous robotic solutions for covering critical production sectors. Agriculture can be seen as such, as its digital transformation is a promising necessity for protecting the environment, and for tackling the degradation of natural resources and increasing nutritional needs of the population on Earth. Many studies focus on the potential of agricultural robotic vehicles to perform operations of increased intelligence. In parallel, the study of the activity footprint of these vehicles can be the basis for supervising, detecting the malfunctions, scaling up, modeling, or optimizing the related operations. In this regard, this work, employing a prototype lightweight autonomous electric cargo vehicle, outlines a simple and cost-effective mechanism for a detailed robot’s power consumption logging. This process is conducted at a fine time granularity, allowing for detailed tracking. The study also discusses the robot’s energy performance across various typical agricultural field operation scenarios. In addition, a comparative analysis has been conducted to evaluate the performance of two different types of batteries for powering the robot for all the operation scenarios. Even non-expert users can conduct the field operation experiments, while directions are provided for the potential use of the data being collected. Given the linear relationship between the size and the consumption of electric robotic vehicles, the energy performance of the prototype agricultural cargo robot can serve as a basis for various studies in the area.
Generating Wall-to-Wall Canopy Height Information from Discrete Data Provided by Spaceborne LiDAR System
Authors: Doyog Nova D. , Lin Chinsu
Published: March 2024

Provision of multi-temporal wall-to-wall canopy height information is one of the initiatives to combat deforestation and is necessary in strategizing forest conversion and reforestation initiatives. This study generated wall-to-wall canopy height information of the subtropical forest of Lishan, Taiwan, using discrete data provided by spaceborne LiDARs, wall-to-wall passive and active remote sensing imageries, topographic data, and machine learning (ML) regression models such as gradient boosting (GB), k-nearest neighbor (k-NN), and random forest (RF). ICESat-2- and GEDI-based canopy height data were used as training data, and medium-resolution passive satellite image (Sentinel-2) data, active remote sensing data such as synthetic aperture radar (SAR), and topographic data were used as regressors. The ALS-based canopy height was used to validate the models’ performance using root mean square error (RMSE) and percentage RMSE (PRMSE) as validation criteria. Notably, GB displayed the highest accuracy among the regression models, followed by k-NN and then RF. Using the GEDI-based canopy height as training data, the GB model can achieve optimum accuracy with an RMSE/PRMSE of 8.00 m/31.59%, k-NN can achieve an RMSE/PRMSE of as low as 8.05 m/31.78%, and RF can achieve optimum RMSE/PRMSE of 8.16 m/32.24%. If using ICESat-2 data, GB can have an optimum RMSE/PRMSE of 13.89 m/54.86%; k-NN can have an optimum RMSE/PRMSE of 14.32 m/56.56%, while RF can achieve an RMSE/PRMSE of 14.72 m/58.14%. Additionally, integrating Sentinel-1 with Sentinel-2 data improves the accuracy of canopy height modeling. Finally, the study underlined the crucial relevance of correct canopy height estimation for sustainable forest management, as well as the potential ramifications of poor-quality projections on a variety of biological and environmental factors.
Physico-Mechanical and Energy Properties of Pine (Pinus sylvestris) and Beech (Fagus sylvatica) Wood from Railroad Ties
Authors: Aniszewska Monika , Gendek Arkadiusz , Tamelová Barbora , Malaťák Jan , Velebil Jan , Krilek Jozef , Čabalová Iveta , Mikłaszewicz Ireneusz , Zychowicz Witold , Drożdżek Michał , Mazurek Andrzej
Published: March 2024

The objective of the present work was to determine the physico-mechanical and energy properties of pine (Pinus sylvestris) and beech (Fagus sylvatica) wood from railroad ties. The ties were divided into internal and external parts as well as into parts impregnated and unimpregnated with creosote oil. The effects of creosote impregnation on wood hardness, compressive strength parallel to the grain, static bending strength, and calorific value were studied. The obtained results show that the parameters of the analyzed samples meet the standard requirements (EN 338) for construction wood (compressive and bending strength class: C50—pine; D70—beech). Depending on the particular property being studied, both pine and beech samples belong to the highest or one of the highest wood quality classes. Creosote oil considerably increased wood density (by 9% for beech and 19% for pine) but did not affect its hardness. Creosote impregnation significantly improved the compressive strength parallel to the grain of both wood species (beech: σc=51.99 MPa (IN); σc=57.78 MPa (OUT); pine: σc=36.56 MPa (IN); σc=42.45 MPa (OUT)); in the case of static bending strength, its value was increased for beech wood (σg=106.13 MPa (IN); σg=113.18 MPa (OUT)) and reduced for pine wood (σg=66.34 MPa (IN); σg=82.62 MPa (OUT)). The oil contained in wood from ties significantly elevated its calorific value (by 25% for beech and 10% for pine). Unfortunately, the presence of creosote oil currently prevents wood from railroad ties from being reused as the oil is deemed hazardous and carcinogenic. However, if it were possible to isolate the unimpregnated parts of railroad ties, they could be reapplied for construction or other uses.
Dragon_Pi: IoT Side-Channel Power Data Intrusion Detection Dataset and Unsupervised Convolutional Autoencoder for Intrusion Detection
Authors: Lightbody Dominic , Ngo Duc-Minh , Temko Andriy , Murphy Colin C. , Popovici Emanuel
Published: March 2024

The growth of the Internet of Things (IoT) has led to a significant rise in cyber attacks and an expanded attack surface for the average consumer. In order to protect consumers and infrastructure, research into detecting malicious IoT activity must be of the highest priority. Security research in this area has two key issues: the lack of datasets for training artificial intelligence (AI)-based intrusion detection models and the fact that most existing datasets concentrate only on one type of network traffic. Thus, this study introduces Dragon_Pi, an intrusion detection dataset designed for IoT devices based on side-channel power consumption data. Dragon_Pi comprises a collection of normal and under-attack power consumption traces from separate testbeds featuring a DragonBoard 410c and a Raspberry Pi. Dragon_Slice is trained on this dataset; it is an unsupervised convolutional autoencoder (CAE) trained exclusively on held-out normal slices from Dragon_Pi for anomaly detection. The Dragon_Slice network has two iterations in this study. The original achieves 0.78 AUC without post-processing and 0.876 AUC with post-processing. A second iteration of Dragon_Slice, utilising dropout to further impede the CAE’s ability to reconstruct anomalies, outperforms the original network with a raw AUC of 0.764 and a post-processed AUC of 0.89.
Effects of Genotype on Pig Carcass, Meat Quality and Consumer Sensory Evaluation of Loins and Bellies
Authors: Razmaitė Violeta , Šveistienė Rūta , Šiukščius Artūras
Published: March 2024

The objective of the study was to compare carcass and meat quality among Lithuanian White, Lithuanian Indigenous Wattle and conventional hybrids as well as consumer sensory evaluations. The pigs were slaughtered at a live weight of approximately 95–100 kg. After 24 h of slaughter, carcasses were evaluated and sampled for the analysis of meat quality traits of loins and consumer evaluation of cooked loins and baked bellies. The pigs of both Lithuanian breeds had higher backfat thickness and depth of underbelly but lower loin area compared with hybrids. However, more fatty local breeds had higher intramuscular fat content but lower cholesterol content than hybrids. The loins from local pigs displayed higher pH and colour with lower lightness and yellowness but higher redness and also lower cooking loss, shear force and hardness than conventional hybrids. Pork from lean hybrids had a higher proportion of polyunsaturated fatty acids and more favourable lipid quality indices such as atherogenic (AI) and thrombogenic (TI) indices and the hypocholesterolemic/hypercholesterolemic (h/H) ratio; however, the peroxidisability index (PI) and iodine value (IV) were less favourable compared with local pigs. Consumers evaluated cooked loins and baked bellies. The loins from local breeds scored higher in juiciness, taste and overall acceptability compared with conventional hybrids. However, a higher overall acceptability was observed for the lean bellies of hybrids. The results can be used to increase pork consumption choices.
Effects of Supplementing Selenium-Enriched Cardamine violifolia to Laying Hens on Egg Quality and Yolk Antioxidant Capacity during Storage at 4 °C and 25 °C
Authors: Qin Kun , Cong Xin , Wang Hui , Yan Mengke , Xu Xianfeng , Liu Mingkang , Song Fulong , Wang Dan , Xu Xiao , Zhao Jiangchao , Cheng Shuiyuan , Liu Yulan , Zhu Huiling
Published: March 2024

Oxidative stress occurs in the process of egg storage. Antioxidants as feed additives can enhance egg quality and extend the shelf life of eggs. Selenium-enriched Cardamine violifolia (SEC) has strongly antioxidant properties. The objective of this study was to assess the effects of dietary supplementation with SEC on egg quality and the yolk antioxidant capacity of eggs stored at 4 °C and 25 °C. Four hundred fifty 65-week-old, Roman hens that were similar in laying rate (90.79 ± 1.69%) and body weight (2.19 ± 0.23 kg) were divided into 5 groups. The birds were fed diets supplemented with 0 mg/kg selenium (Se) (CON), 0.3 mg/kg Se from sodium selenite (SS), 0.3 mg/kg Se from Se-enriched yeast (SEY), 0.3 mg/kg Se for selenium-enriched Cardamine violifolia (SEC) or 0.3 mg/kg Se from Se-enriched Cardamine violifolia and 0.3 mg/kg Se from Se-enriched yeast (SEC + SEY) for 8 weeks. The eggs were collected on the 8th week and were analyzed for egg quality and oxidative stability of yolk during storage at 4 °C or 25 °C for 0, 2, 4, or 6 weeks. Dietary SEC and SEC + SEY supplementation increased the Haugh unit (HU) and albumen foam stability in eggs stored at 4 °C and 25 °C (p < 0.05). SS and SEC supplementation increased the yolk index in eggs stored at 25 °C (p < 0.05). SEC or SEC + SEY slowed down an increase in albumen pH and gel firmness in eggs stored at 4 °C and 25 °C (p < 0.05). Moreover, SEC or SEC + SEY alleviated the increase in malonaldehyde (MDA), and the decrease in total antioxidant capacity (T-AOC) level and total superoxide dismutase (T-SOD) activity in yolks stored at 4 °C and 25 °C (p < 0.05). These results indicate that SEC mitigated egg quality loss and improved the antioxidant capacity of yolks during storage. SEC supplementation would be advantageous to extend the shelf life of eggs.
Nutritional Quality and Safety Assessment of Pork Meat Cuts from Romania: Fatty Acids and Elemental Profile
Authors: Covaciu Florina-Dorina , Feher Ioana , Cristea Gabriela , Dehelean Adriana
Published: March 2024

In this study, the fatty acids and elemental profiles of 53 pork cut samples were determined. To offer insights into their potential health implications, we computed 18 key nutritional indices. These indices included parameters such as saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), unsaturated fatty acids (UFAs), the MUFAs/SFAs ratio, PUFAs/SFAs ratio, atherogenic index (AI), thrombogenic index (TI), the hypocholesterolemic to hypercholesterolemic ratio (h/H), health-promoting index (HPI), hypocholesterolemic index (HI), unsaturation index (UI), saturation index (SI), peroxidizability index (PI), nutritional value index (NVI), hypocholesterolemic index of fatty acids (DFAs), hypercholesterolemic index of fatty acids (OFAs), and the DFAs/OFAs ratio. These indices were calculated based on their fatty acid composition to provide comprehensive nutritional information. A health risk assessment revealed the safety and minimum health risk for the population from consuming the investigated pork cuts using the Target Hazard Quotient (THQ), Hazard Index (HI), and target cancer risk (TR). The ANOVA test showed significant differences in the levels of K, Fe, Mn, Zn, MUFAs, and AI among the pork cut samples. It was noted that by employing the correlation between the fatty acids profile, nutritional indices, and elemental concentrations and an unsupervised statistical method, such as PCA, a perfect separation from the different pork cuts could not be obtained.
Sexual Minorities and Loneliness: Exploring Sexuality through Social Media and Gender–Sexuality Alliance (GSA) Supports
Authors: Charmaraman Linda , Zhang Alice , Wang Kaitlyn , Chen Becky
Published: March 2024

We examined online and offline social supports for sexual minority adolescents, underscoring the understudied developmental period of early adolescence and the mental outcome of loneliness. Stemming from a larger study in the northeast U.S., 967 youth participants were 26% sexual minority, 53% female, 45% male, and 2% other/nonbinary (mean age = 13.1, SD = 1.52). LGBTQ+ youth reported significantly higher levels of loneliness compared to their heterosexual counterparts. To understand potential sources of social support while exploring their sexual identities, we compared the experiences of LGBTQ+ youth at both ends of the loneliness spectrum. Gaining knowledge about their sexual orientation from LGBTQ+ organization websites, participating in gender–sexuality alliances, and using TikTok or Instagram were associated with lower levels of loneliness. Providing social support to online friends was associated with lower loneliness; however, receiving online support was not associated with lower loneliness. Furthermore, proactive social media engagement such as posting uplifting content, joining online communities, or raising awareness about social issues were associated with lower levels of loneliness. The results provide guidance on specific youth behaviors and online communities beyond a focus on screen time while highlighting the continued need for social support to ameliorate loneliness, such as gender–sexuality alliance networks.
Othering Older People’s Housing: Gaming Ageing to Support Future-Planning
Authors: McCall Vikki , Rutherford Alasdair C. , Bowes Alison , Jagannath Sadhana , Njoki Mary , Quirke Martin , Pemble Catherine M. , Lovatt Melanie , Davison Lisa , Maginn Katie , Scrutton Pat , Pengelly Ro , Gibson Joan
Published: March 2024

The ‘othering’ of ageing is linked to an integrated process of ageism and hinders planning for the future for both individuals and practitioners delivering housing and health services. This paper aims to explore how creative interventions can help personalise, exchange knowledge and lead to system changes that tackle the ‘othering’ of ageing. The Designing Homes for Healthy Cognitive Ageing (DesHCA) project offers new and creative insights through an innovative methodology utilising ‘serious games’ with a co-produced tool called ‘Our House’ that provides insights into how to deliver housing for older people for ageing well in place. In a series of playtests with over 128 people throughout the UK, the findings show that serious games allow interaction, integration and understanding of how ageing affects people professionally and personally. The empirical evidence highlights that the game mechanisms allowed for a more in-depth and nuanced consideration of ageing in a safe and creative environment. These interactions and discussions enable individuals to personalise and project insights to combat the ‘othering’ of ageing. However, the solutions are restrained as overcoming the consequences of ageism is a societal challenge with multilayered solutions. The paper concludes that serious gaming encourages people to think differently about the concept of healthy ageing—both physically and cognitively—with the consideration of scalable and creative solutions to prepare for ageing in place.
Structured Tandem Repeats in Protein Interactions
Authors: Mac Donagh Juan , Marchesini Abril , Spiga Agostina , Fallico Maximiliano José , Arrías Paula Nazarena , Monzon Alexander Miguel , Vagiona Aimilia-Christina , Gonçalves-Kulik Mariane , Mier Pablo , Andrade-Navarro Miguel A.
Published: March 2024

Tandem repeats (TRs) in protein sequences are consecutive, highly similar sequence motifs. Some types of TRs fold into structural units that pack together in ensembles, forming either an (open) elongated domain or a (closed) propeller, where the last unit of the ensemble packs against the first one. Here, we examine TR proteins (TRPs) to see how their sequence, structure, and evolutionary properties favor them for a function as mediators of protein interactions. Our observations suggest that TRPs bind other proteins using large, structured surfaces like globular domains; in particular, open-structured TR ensembles are favored by flexible termini and the possibility to tightly coil against their targets. While, intuitively, open ensembles of TRs seem prone to evolve due to their potential to accommodate insertions and deletions of units, these evolutionary events are unexpectedly rare, suggesting that they are advantageous for the emergence of the ancestral sequence but are early fixed. We hypothesize that their flexibility makes it easier for further proteins to adapt to interact with them, which would explain their large number of protein interactions. We provide insight into the properties of open TR ensembles, which make them scaffolds for alternative protein complexes to organize genes, RNA and proteins.
Study on the Mechanism of the Adrenaline-Evoked Procoagulant Response in Human Platelets
Authors: Gołaszewska Agata , Misztal Tomasz , Kazberuk Adam , Rusak Tomasz
Published: March 2024

Adrenaline has recently been found to trigger phosphatidylserine (PS) exposure on blood platelets, resulting in amplification of the coagulation process, but the mechanism is only fragmentarily established. Using a panel of platelet receptors’ antagonists and modulators of signaling pathways, we evaluated the importance of these in adrenaline-evoked PS exposure by flow cytometry. Calcium and sodium ion influx into platelet cytosol, after adrenaline treatment, was examined by fluorimetric measurements. We found a strong reduction in PS exposure after blocking of sodium and calcium ion influx via Na+/H+ exchanger (NHE) and Na+/Ca2+ exchanger (NCX), respectively. ADP receptor antagonists produced a moderate inhibitory effect. Substantial limitation of PS exposure was observed in the presence of GPIIb/IIIa antagonist, phosphoinositide-3 kinase (PI3-K) inhibitors, or prostaglandin E1, a cyclic adenosine monophosphate (cAMP)-elevating agent. We demonstrated that adrenaline may develop a procoagulant response in human platelets with the substantial role of ion exchangers (NHE and NCX), secreted ADP, GPIIb/IIIa-dependent outside-in signaling, and PI3-K. Inhibition of the above mechanisms and increasing cytosolic cAMP seem to be the most efficient procedures to control adrenaline-evoked PS exposure in human platelets.
The Efficacy of Stem Cells in Wound Healing: A Systematic Review
Authors: Farabi Banu , Roster Katie , Hirani Rahim , Tepper Katharine , Atak Mehmet Fatih , Safai Bijan
Published: March 2024

Wound healing is an intricate process involving coordinated interactions among inflammatory cells, skin fibroblasts, keratinocytes, and endothelial cells. Successful tissue repair hinges on controlled inflammation, angiogenesis, and remodeling facilitated by the exchange of cytokines and growth factors. Comorbid conditions can disrupt this process, leading to significant morbidity and mortality. Stem cell therapy has emerged as a promising strategy for enhancing wound healing, utilizing cells from diverse sources such as endothelial progenitor cells, bone marrow, adipose tissue, dermal, and inducible pluripotent stem cells. In this systematic review, we comprehensively investigated stem cell therapies in chronic wounds, summarizing the clinical, translational, and primary literature. A systematic search across PubMed, Embase, Web of Science, Google Scholar, and Cochrane Library yielded 22,454 articles, reduced to 44 studies after rigorous screening. Notably, adipose tissue-derived mesenchymal stem cells (AD-MSCs) emerged as an optimal choice due to their abundant supply, easy isolation, ex vivo proliferative capacities, and pro-angiogenic factor secretion. AD-MSCs have shown efficacy in various conditions, including peripheral arterial disease, diabetic wounds, hypertensive ulcers, bullous diabeticorum, venous ulcers, and post-Mohs micrographic surgery wounds. Delivery methods varied, encompassing topical application, scaffold incorporation, combination with plasma-rich proteins, and atelocollagen administration. Integration with local wound care practices resulted in reduced pain, shorter healing times, and improved cosmesis. Stem cell transplantation represents a potential therapeutic avenue, as transplanted stem cells not only differentiate into diverse skin cell types but also release essential cytokines and growth factors, fostering increased angiogenesis. This approach holds promise for intractable wounds, particularly chronic lower-leg wounds, and as a post-Mohs micrographic surgery intervention for healing defects through secondary intention. The potential reduction in healthcare costs and enhancement of patient quality of life further underscore the attractiveness of stem cell applications in wound care. This systematic review explores the clinical utilization of stem cells and stem cell products, providing valuable insights into their role as ancillary methods in treating chronic wounds.
Correction: Lozano-Soria et al. Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (Cosmopolites sordidus). Insects 2020, 11, 509
Authors: Lozano-Soria Ana , Picciotti Ugo , Lopez-Moya Federico , Lopez-Cepero Javier , Porcelli Francesco , Lopez-Llorca Luis Vicente
Published: March 2024

In the original publication [...]
Gaucher Disease or Acid Sphingomyelinase Deficiency? The Importance of Differential Diagnosis
Authors: Giacomarra Miriam , Colomba Paolo , Francofonte Daniele , Zora Marcomaria , Caocci Giovanni , Diomede Daniela , Giuffrida Gaetano , Fiori Laura , Montanari Chiara , Sapuppo Annamaria , Scortechini Anna Rita , Vitturi Nicola , Duro Giovanni , Zizzo Carmela
Published: March 2024

Background: Gaucher disease is a lysosomal storage disorder caused by functional glucocerebrosidase enzyme deficiency. Hepatosplenomegaly and hematological complications are found in both Gaucher disease and Acid Sphingomyelinase Deficiency, which is caused by acid sphingomyelinase dysfunction. The possible overlap in clinical presentation can cause diagnostic errors in differential diagnosis. For this reason, in patients with an initial clinical suspicion of Gaucher disease, we aimed to carry out a parallel screening of acid sphingomyelinase and glucocerebrosidase. Methods: Peripheral blood samples of 627 patients were collected, and enzymatic activity analysis was performed on both glucocerebrosidase and acid sphingomyelinase. The specific gene was studied in samples with null or reduced enzymatic activity. Specific molecular biomarkers helped to achieve the correct diagnosis. Results: In 98.7% of patients, normal values of glucocerebrosidase activity excluded Gaucher disease. In 8 of 627 patients (1.3%), the glucocerebrosidase enzymatic activity assay was below the normal range, so genetic GBA1 analysis confirmed the enzymatic defect. Three patients (0.5%) had normal glucocerebrosidase activity, so they were not affected by Gaucher disease, and showed decreased acid sphingomyelinase activity. SMPD1 gene mutations responsible for Acid Sphingomyelinase Deficiency were found. The levels of specific biomarkers found in these patients further strengthened the genetic data. Conclusions: Our results suggest that in the presence of typical signs and symptoms of Gaucher disease, Acid Sphingomyelinase Deficiency should be considered. For this reason, the presence of hepatosplenomegaly, thrombocytopenia, leukocytopenia, and anemia should alert clinicians to analyze both enzymes by a combined screening. Today, enzyme replacement therapy is available for the treatment of both pathologies; therefore, prompt diagnosis is essential for patients to start accurate treatment and to avoid diagnostic delay.
The Role of IVC Filters in the Management of Acute Pulmonary Embolism
Authors: Asmar Samer , Michael George , Gallo Vincent , Weinberg Mitchell D.
Published: March 2024

Venous thromboembolism (VTE), comprising deep venous thrombosis (DVT) and pulmonary embolism (PE), is a prevalent cardiovascular condition, ranking third globally after myocardial infarction and stroke. The risk of VTE rises with age, posing a growing concern in aging populations. Acute PE, with its high morbidity and mortality, emphasizes the need for early diagnosis and intervention. This review explores prognostic factors for acute PE, categorizing it into low-risk, intermediate-risk, and high-risk based on hemodynamic stability and right ventricular strain. Timely classification is crucial for triage and treatment decisions. In the contemporary landscape, low-risk PE patients are often treated with Direct Oral Anticoagulants (DOACS) and rapidly discharged for outpatient follow-up. Intermediate- and high-risk patients may require advanced therapies, such as systemic thrombolysis, catheter-directed thrombolysis, mechanical thrombectomy, and IVC filter placement. The latter, particularly IVC filters, has witnessed increased usage, with evolving types like retrievable and convertible filters. However, concerns arise regarding complications and the need for timely retrieval. This review delves into the role of IVC filters in acute PE management, addressing their indications, types, complications, and retrieval considerations. The ongoing debate surrounding IVC filter use, especially in patients with less conventional indications, reflects the need for further research and data. Despite complications, recent studies suggest that clinically significant issues are rare, sparking discussions on the appropriate and safe utilization of IVC filters in select PE cases. The review concludes by highlighting current trends, gaps in knowledge, and potential avenues for advancing the role of IVC filters in future acute PE management.
The Management of a Geriatric Patient Using Dabigatran Therapy on Dentigerous Cyst with Oral Bleeding
Authors: Chisci Glauco , Chisci Dafne , Chisci Enea , Chisci Viola , Stumpo Michela , Chisci Elettra
Published: March 2024

Jaw cysts represent a great matter of interest in oral and maxillofacial surgery. Jaw cyst surgery is a common intervention in oral surgery but, in the case of a multidisciplinary patient, the oral surgeon needs to meet with other specialists. A cyst is an epithelium-lined sac containing fluid and/or semisolid material due to epithelial cell proliferation, degeneration, and liquefaction; the hypertonic solution withdraws liquids from the surrounding tissues, while internal pressure exerts an equal strength on the cyst walls. Dentigerous cysts are the second most common odontogenic cysts after radicular cysts, and commonly few or no symptoms are reported. However, the most common diagnosis for dentigerous cyst is represented by eruption of the affected tooth or accidental diagnosis. Commonly, dentigerous cysts may be related to impacted third molars; in the case of impacted third molars and a dentigerous cyst, the tooth should be removed along with the cyst in the same intervention. Mandibular dentigerous cysts are common in children and adults, while dentigerous cysts are a rare neoformation in elderly patients. Treatment usually involves removal of the entire cyst and the associated unerupted tooth. This intervention may be more difficult if the cyst is large, the third molar is in contact with the mandibular nerve, and/or the patient has a medical history that may represent a relative or absolute contraindication. We present the case of a rare symptomatic manifestation of dentigerous cyst in an elderly patient in treatment with dabigatran therapy; for the treatment of dentigerous cysts in the elderly, we suggest a multidisciplinary approach with the use of the histological examination and a careful follow-up.