bluuebunny/crossref_metadata_2025 huggingface dataset:
TRAUMA-SENSITIVE PEDAGOGY & PRACTICE NEWSLETTER 1 (OF 2)
Authors: Fidyk Alexandra , Krahn Mandy , Balinska-Ourdeva Vessela , Jacobsen Karen , Brooks-Starks Alison
Published: October 2020

This publication includes the first of two newsletters published in this issue of Art/Research International. This second newsletter is followed by a commentary and references for both newsletters.

Attentive to local and global mental health realities and the emergent need to provide intercultural mental health perspectives, resources, and methods that work across cultures in school contexts, I (the first author) conducted a participatory poetic inquiry, “Image, Body, and Voice: Supporting Girls’ Sense of Wellbeing,” with grade-6 girls in an inner-city school in Alberta. It sought to: (i) meet new Teacher Quality Standards (TQS) “to build positive and productive relationships with students [and] peers” (AB Education, 2018, p. 4); and (ii) be “aware of and facilitate responses to the emotional and mental health needs of students” (p. 6). I was guided by the following research question: In what ways might girls’ experiences with art-integrated activities and body-centred methods inform educators about pedagogical practice and mental health interventions?

Findings indicated the transdisciplinary praxis that emerged—arts-based, contemplative and somatic methods—enhanced the girls’ sense of self and wellbeing. The youth reported that these activities had explicit value: sharing circles used for check-ins and - outs, ceremony—which welcomed witnessing—relational and body-centred practices, and one-on-one time with the PI. The life-size body maps as research creation illustrated that the participants learned to externalize sensations and emotions in a safe way, aiding them in the development of skills needed for emotional self-regulation. Body maps broadly defined are life-size body images, while body mapping is the process of creating body maps using collage, photography, painting, or other arts-integrated methods to visually symbolize aspects of people’s lives, their bodies, and their worlds.

Funding from Research Impact Canada, VP Research & Innovation University of Alberta and the Kule Institute for Advanced Study aided to mobilize evidence-informed knowledge from this research through professional community engagement with pre- service teachers at a full-day workshop. Presenters at the workshop were members of a Community of Professional Practice (COPP) where the activities of educational research and trauma-sensitive practices were shared, including culturally aware methods for diverse populations. This newsletter reflects one of two events and two research creation artifacts provided as follow up to the attendees.
Educação ambiental não-formal: a experiência dos parques urbanos de Goiânia
Authors: Silva Ariana Cárita de Assis Marinho , Oliveira Leandro Gonçalves
Published: October 2020

O enfrentamento dos problemas ambientais exige uma transformação na relação entre a humanidade e natureza. A Educação Ambiental não-formal é uma estratégia importante para uma reintegração do ser humano ao ambiente, despertando sujeitos cidadãos com consciência planetária para intervir nas questões socioambientais. Nessa perspectiva, esta pesquisa, desenvolvida em sete parques da cidade de Goiânia, teve como objetivo estudar a educação ambiental nesses ambientes, avaliando a forma como as políticas públicas são consolidadas e explorando as percepções dos sujeitos vinculados a essas unidades. Trata-se de uma análise qualitativa por meio da categorização das respostas de questionários e entrevistas, além da análise de documentos da gestão pública municipal. Embora tenha se constatado uma percepção conflituosa entre natureza e ambiente, foi possível identificar elementos para ressignificação dos parques como espaços educativos para exercício da cidadania.
To break “the Black Wall”. The motif of fear in plays by Henri-René Lenormand
Authors: Kaczmarek Tomasz
Published: October 2020

Henri-René Lenormand refreshed theatre, defining a new domain for it: the mysteries of the human soul. In all of his plays, he strived to explain the secret of internal life, as well as to solve the mystery that people are to themselves. Therefore, dramaturgy was for the author of La Folle du Ciel not only a means of literary expression, but also a kind of therapy, enabling him to combat his depression. In this article, three plays are discussed: Le Temps est un songe, Les Ratés, and Le Lâche, in which the French playwright diagnosed cases of melancholia by describing the psychotic world from the perspectives of the suffering protagonists. He presented them in closure, isolated from the rest of the world, suffocating in claustrophobic rooms under mansard roofs which symbolised their strained mental conditions. Apart from physical walls, in Lenormand’s works there is also the invisible to the eye yet pervasive “black wall”, in front of which a human being stands completely defenceless and mentally broken, trying to find in it even the slightest crack enabling them to escape the delusional world.
Evaluación fisicoquímica y biológica en sistemas de tratamiento comerciales en Costa Rica
Authors: Calvo-Brenes Guillermo , Zambrano Diana , Picado-Valverde Sofía
Published: October 2020

En Costa Rica, el 70.9% de la población utiliza el tanque séptico como sistema de tratamiento individual a las aguas residuales domésticas. A pesar de que los tanques sépticos son la tecnología de tratamiento más utilizada en el país, no se cuenta con información detallada acerca de su funcionamiento y su eficiencia. Además, labores de mantenimiento, como lo es la remoción de lodos sépticos, son ejecutadas directamente por cada familia que usa este sistema y sin que exista un protocolo de limpieza unificado. La evaluación de estos sistemas se llevó a cabo en sistemas de tratamiento fabricados por una de las empresas privadas más importantes del país. Cada equipo evaluado consistía en un tanque séptico y un filtro anaerobio de flujo ascendente (FAFA) para el tratamiento de aguas residuales domésticas. La caracterización incluyó aspectos físicos, químicos y biológicos de la calidad y cantidad del agua residual producida en tres sistemas de tratamiento distintos, considerando variaciones horarias, así como la calidad y la cantidad de lodos acumulados en cada sistema. Los sistemas de tratamiento estaban instalados en dos edificios multifamiliares y una casa unifamiliar. Los resultados indicaron que los sistemas de tratamiento son muy sensibles en cuanto a cantidad y calidad del agua residual, presentando variaciones horarias significativas y además, afectadas por el tipo de uso de agua que se da en los hogares; lo cual está directamente relacionado al funcionamiento del tratamiento.
Uso de vehículos aéreos no tripulados (VANTs) para el monitoreo y manejo de los recursos naturales: una síntesis
Authors: Guevara-Bonilla Mario , Meza-Leandro Ana Sofía , Esquivel-Segura Edwin Antonio , Arias-Aguilar Dagoberto , Tapia-Arenas Andrea , Masís Meléndez Federico
Published: October 2020

El desarrollo tecnológico de los vehículos aéreos no tripulados, VANT´s da acceso a nuevas aplicaciones en múltiples campos de la ciencia y la ingeniería. El uso de sensores remotos, el análisis satelital, la medición de datos en el sitio han sido las principales herramientas empleadas para la monitorización y el estudio de los recursos naturales. Los VANT´s son una tecnología de bajo costo capaz de recolectar información de manera rápida y precisa, con alta resolución espacial. El objetivo del presente trabajo fue realizar una revisión de las características principales y las aplicaciones de los vehículos aéreos no tripulados en el manejo de los recursos naturales, principalmente en la región latinoamericana. En este trabajo se describen los tipos de drones existentes, sus ventajas, desventajas y los diferentes tipos de sensores que se pueden adaptar. Además, se revisaron trabajos sobre VANTS realizados en Brasil, Perú y Colombia como una guía para América Latina. Se muestra una   serie de posibles arreglos entre VANTS y sensores remotos., Próximamente, estos arreglos tendrán un amplio potencial de incrementar la eficiencia de adquisición de datos, incrementando su aplicabilidad en el campo del estudio de los recursos naturales. El futuro del uso de este tipo de tecnologías parece apuntar a una mayor automatización en la captura de datos, mejoras en el rendimiento de los tiempos de vuelo y sistemas automatizados con algoritmos complejos capaces de ofrecer información en tiempo real.
Differentiated instruction in the mathematics classroom: Teachers’ teaching experience in a teacher professional development
Authors: Hidayati Fina Hanifa
Published: October 2020

Differentiated instruction is an approach that can be used to facilitate students with various characteristics and help them to master mathematics in every topic learned. This study describes teachers’ performance in implementing differentiated instruction. In the second secondary school level, thirty mathematics teachers designed mathematics lessons using differentiated instruction in different topics, peer-reviewed, and peer-presented. Implementing the lesson in a real classroom was done while conducting classroom observation to see what works well during the learning process and improve it. Qualitative descriptive method was used to describe the process of research and analyze findings during the lesson through observation and forum group interview. From this process, teachers prepared instruction based on students’ abilities, took into account personal mathematics understanding, and helped students achieve mathematics learning goals. Teachers also learned strategies for helping students learned. Meanwhile, students enjoyed the learning process through their different ways of learning.
Pengaruh Kepemimpinan Islami dan Budaya Organisasi Islami Terhadap Motivasi Kerja Islami Pada UMKM Kulit di Magetan
Authors: Aziz Rezy , Shofawati Atina
Published: October 2020

The purpose of this research is to know the effect of islamic leadership and islamic organization culture in variable through islamic work motivation to SMEs leather in Magetan.This research uses questionnaires in primary data collected and the method used is quantitative descriptive by using two independent variables islamic leadership and islamic organization culture and a dependent variable islamic work motivation. Research sampling is the employee of SMEs leather in Magetan industry area in east java, it was 110 people. Sampling collected takes in proportioned stratified random sampling because each SMEs leather will be taken a random sampling. Data analysis technique used is multiple linear regression analysis by using F and T treatment as hypothesisThe results showed that simultaneous and partial Islamic leadership and Islamic organizational culture influence significant on Islamic work motivation.
Faktor Yang Mempengaruhi Keputusan Mahasiswa S1 Ekonomi Islam Fakultas Ekonomi dan Bisnis Universitas Airlangga Belum Menjadi Nasabah Bank Syariah
Authors: Johan Mega Nilasari , Ratnasari Ririn Tri
Published: October 2020

This research aims to determine the factors that influence the decision of Students of Islamic Economics FEB UNAIR not selected as Syariah Bank’s Customer.This research used quantitative methods through exploratory factor analysis. Characteristic of the research population are students of Islamic Economics UNAIR who following the active class. The number of samples in pre eliminary test by conducting a written interview by 25 respondents, and main test required 100 respondents. The sampling technique used is probability sampling. Sampling was done using simple random sampling.Based on the results of the study indicate that there are four factors that affect the decision of Students of Islamic Economics FEB UNAIR. The four factors as a result of reduction of processing factor analysis. These four factors named by factor of the brand image, product knowledge, spurious loyalty, service experience.Advice for the Syariah Bank are improves service experience for every remittance transactions by ontime, Syariah Bank offers interesting sales promotion to get customers, more socialization about negative impact of riba, not burden charge for every transactions in ATM or online banking. Advice for the next researcher, can analys factors made from this research influencing other variabel that decision muslim students S1 FEB UNAIR have not been Syariah Bank’s customer yet, for the second next research, make the factors in this research being exogen variables that influenced muslim consumen behavior to Syariah Bank.
Implementasi Konsumsi Islami Pada Pengajar Pondok Pesantren (Studi Kasus Pada Pengajar Pondok Pesantren Al Aqobah Kecamatan Diwek Kabupaten Jombang)
Authors: Baidhowi Bagus , Zaki Irham
Published: October 2020

This study aims to determine the application of Islamic consumption by Al Aqobah Boarding School teacher in Diwek Subdistrict, Jombang District.This study used a qualitative approach with descriptive case study method. The population characteristics of this study are the teachers of Al Aqobah Boarding School in Diwek Subdistrict, Jombang District. The teachers of Al Aqobah Boarding School who know and understand the consumption according to Islam are people who implement Islamic consumption in his life, the teacher/ustadz and ustadzah who has been teaching at Al Aqobah boarding school at least 2 years. The number of samples in this study was six informants. The sampling technique used was purposive sampling.Based on the results of this study showed that Al Aqobah boarding school teacher in Diwek Subdistrict, Jombang District, has implemented the Islamic consumption legally (halal); they are not extravagant not only in spending wealth but also in living luxury and showing assets. They can also make the balance system between worldly and hereafter consumption.Moreover, the suggestion for all of people in Al Aqobah boarding school and also the teachers are expected to continue teaching the consumption which is true to Islam, apply in everyday life, and provide appropriate examples to the students. For further research, it is expected to examine the same topic with more in depth details about the implementation of Islamic consumption on other aspects that are more specific.
Implementasi Sifat Amanah Pengelola Koperasi Pondok Pesantren (Studi Kasus Pada Pengelola Koperasi Pondok Pesantren Qomaruddin Kecamatan Bungah Kabupaten Gresik
Authors: Abiyoga Firdaus Arfianandy , Zaki Irham
Published: October 2020

A Muslim who works as a form of workship to Allah SWT make attempts to obtain wealth by means of the lawful and good. As well as within the organization, a Muslim who gained the trust of the other party will always manage cooperatives by prioritizing and applying traits according to traits owned by Rasulallah, one of which is the trusthworthy traits and is able to provide a positive influence to others within the organization.The research method used is case study with a qualitative descriptive approach. This study uses interviews in primary data collection. The scope of research is limited to the focus on the cooperative manager of Qomaruddin boarding school Sampurnan Bungah in Gresik and find answers on how to implement the trustworthy traits of Rasulalllah on the management of the cooperative.The result of this research is the implementation of the trustworthy traits of Rasulallah SAW has already been implemented properly by the cooperative manager of the cooperative boarding school in the form of cooperative management in accordance with Islamic principle, because the cooperative management fullfilled with the 3 indicators such as responsibility, trusworthiness and transparency so that the growth and development of cooperative boarding school survived until today. The manager of the boarding school cooperative that implements the trustworthy traits of Rasulallah able to make policy and ethical Islamic system which resulted in an Islamic organization. The cooperative management has implemented the trustworthy traits correctly, by implementing those trustworthy traits, kopontren obtain the benefit. Kopontren manager interpret the business benefits based on the blessing of sustenance and pleasure of Allah SWT from the management as well as the ability and the sincerity to provide benefits to many parties.
Političkoekonomske marginalije o recepciji Marxa u XXI. stoljeću (u povodu čitanja knjige Daga Strpića Robna proizvodnja i udruženi rad u Marxovoj kritici političke ekonomije)
Authors: Kovač Bogomir
Published: October 2020

Političkoekonomskom analizom recepcije Marxa u Strpićevoj knjizi o Marxovoj kritici političke ekonomije autor želi pokazati kakav je suvremeni položaj i koja je moguća aktualnost Marxove analize. Do aktualizacije i povratka Marxu uvijek dolazi u vrijeme kriza, njegova intelektualna sudbina ovisi o preobrazbi sadašnjeg kapitalizma i njegovoj alternativi. Paradoks teorijske nedovršenosti u Marxovoj analizi zbog njegove spoznajne težnje za savršenstvom epistemologije kapitalizma postaje sudbina svih marksističkih interpretacija, pa tako i Strpićeve. Problem Marxa danas je upravo u metafizici teorije vrijednosti i cijeloga robnog sustava koji ne omogućuje ontologijske preobrazbe vrijednosti, kapitala i kapitalizma. Njegov teorijski pristup je više fiziokratski nego novčani, više ekonomski nego politički. Zato Bidetova rekonstrukcija Marxa u okvirima moderne polazi od “metastrukture” općega političkopravnog momenta, koji u marksovskoj kritici nedostaje. Primjeren pristup podrazumijeva poimanje kapitalizma kao eminentno političkoga: politički kapitalizam, kako pokazuje autor, nije poseban oblik kapitalizma, nije historijski vezan uz suvremeni oblik kapitalizma XXI. stoljeća (Kina...), nego je njegova univerzalna povijesna forma. Marxova je kritika političke ekonomije moderna upravo na tom nedovoljno određenom putu njegove analize. Politički kapitalizam kao “kapitalizam općenito” pokazuje svoju relevantnost u analizi suvremenih kriza, koje nisu više samo ekonomske nego ekologijske, pa i pandemijske. U nizu recentnih kriza političkog kapitalizma pokazuje se kako smo suočeni s izborom između realnog autokratskog “barbarskog kapitalizma” i više utopijskog “kozmopolitskog ekosocijalizma”.
Aplicação das Bibliotecas Python para tratamento de dados em tempo real
Authors: Vicentainer Denis , Mattedi Marcos , Mello Bruno
Published: October 2020

A necessidade de monitorar a propagação do Covid-19 (Sars-Cov-2) fez emergir uma demanda, sem precedentes, por armazenamento, tratamento e análise de dados. Esta demanda impõe aos pesquisadores maior celeridade e agilidade no armazenamento, tratamento e disponibilização do dado para visualização da sociedade e de gestores públicos. Para tanto, as ferramentas comuns de manipulação de dados se mostraram pouco eficientes em acompanhar as novas demandas. Considerando estes fatores, o presente artigo objetiva explorar a solução de tratamento e mapeamento de dados de diferentes bases através de bibliotecas Python. A linguagem Python tem se notabilizado por sua variedade de bibliotecas direcionadas para a área de ciência de dados, aumentando sua eficiência enquanto ferramenta de tratamento e análise de dados e estatísticas. Para ilustrar este processo será considerado o de tratamento de dados de isolamento social no Estado de Santa Catarina entre fevereiro e maio de 2020. O texto está dividido em três partes, nas quais: i) pressupostos operacionais e instalação das bibliotecas; ii) limpeza e análise do dado de isolamento social em Santa Catarina e; iii) elaboração do mapeamento, gerando um mapa como produto. Este ensaio possibilitou, de forma prática, descobrir e manipular inconsistências no dado, resultando em um produto mais rápido, assertivo e pronto para ser disponibilizado.
Increased Association of Deamidated αA-N101D with Lens Membrane of Transgenic αAN101D vs. Wild Type αA Mice: Potential Effects on Intracellular Ionic Imbalance and Membrane Disorganization 
Authors: Srivast Om , Srivast Kiran , Joseph Roy , Wilson Landon
Published: October 2020

We have generated two mouse models, in one by inserting the human lens αAN101D transgene in CRYαA N101D mice, and in the other by inserting human wild-type αA-transgene in CRYαA WT mice. The CRYαA N101D mice developed cortical cataract at about 7-months of age relative to CRYαA WT mice. The objective of the study was to determine the following relative changes in the lenses of CRYαA N101D - vs. CRYαA WT mice: age-related changes with specific emphasis on protein insolubilization, relative membrane-association of αA N101D vs. WTαA proteins, and changes in intracellular ionic imbalance and membrane organization. Methods: Lenses of varying ages from CRYαA WT and CRYαA N101D mice were compared for an age-related protein insolubilization. The relative lens membrane-association of the αAN101D- and WTαA proteins in the two types of mice was determined by immunohistochemical-, immunogold-labeling-, and western blot analyses. The relative levels of membrane-binding of recombinant αA N101D - and WTαA proteins was determined by an in vitro assay, and the levels of intracellular Ca 2+ uptake and Na, K-ATPase mRNA were determined in the cultured epithelial cells from lenses of the two types of mice. Results: Compared to the lenses of CRYαA WT , the lenses of CRYαA N101D mice exhibited: (A) An increase in age-related protein insolubilization beginning at about 4-months of age. (B) A greater lens membrane-association of αAN101D- relative to WTαA protein during immunogold-labeling- and western blot analyses, including relatively a greater membrane swelling in the CRYαA N101D lenses. (C) During in vitro assay, the greater levels of binding αAN101D- relative to WTαA protein to membranes was observed. (D) The 75% lower level of Na, K-ATPase mRNA but 1.5X greater Ca 2+ uptake was observed in cultured lens epithelial cells of CRYαA N101D- than those of CRYαA WT mice. Conclusions: The results show that an increased lens membrane association of αA N101D - - relative WTαA protein in CRYαA N101D mice than CRYαA WT mice occurs, which causes intracellular ionic imbalance, and in turn, membrane swelling that potentially leads to cortical opacity.
Assessment of Optometrists’ Knowledge, Skills and Practice on Cataract: A Cross-Sectional Study from Kisumu County, Western Kenya
Authors: Muma Shadrack , Obonyo Stephen
Published: October 2020

Background: The quality of life can be impacted negatively by blindness arising from cataract. The total prevalence of blindness in Kenya is estimated at 0.7%, however cataract contributes almost half (43%) of the total blindness in Kenya. Optometrists are well placed to assess and refer cataract patients. However, little is known on optometrists’ skills, practice and knowledge. Therefore, this study was designed to assess optometrists’ knowledge, skill and practice on cataract in Kisumu, Kenya. Methods: A cross-sectional study design was used. The study was conducted from June 2019 to August 219 using self-administered questionnaire. Basic socio-demographic characteristics were collected and participants’ knowledge, skills and practice on cataract were investigated. The primary outcome measure was the proportions of participants who identified the questions related to knowledge, skills and practice on cataract. Chi-square analysis was performed to assess the association between demographic characteristics of participants with practice, knowledge and skills. Results: A total of 49 optometrists with a mean age of 30.4 years and mean duration of practice of 1-10 years were interviewed. Most optometrists had good knowledge on various aspects of cataract. For example (98%) had a good knowledge on the types of cataract. Almost three quarter (75.5%) of the optometrists reported that they could diagnose cataract correctly based on skills. However, half (57.1%) of the optometrists could not identify nuclear cataract. Being a self reported practice and not an observed practice, most optometrists (61.2%) reported that they did not screen patients aged 40 years and above for cataract. Almost half (52.6%) of the optometrists reported that they did a routine eye examination however, they could not justify the significance of examining the crystalline lens for patient above 40 years. Conclusion The study established that despite the good level of knowledge among optometrist on cataract, there exist a gap on skills and practice. The results of this study calls for more clinical based activities among optometrists. This will eases diagnosis of cataract and its management with an aim to reduce the burden in Kenya.
Relationship between structural and functional changes in glaucomatous eyes: A multifocal electroretinogram study
Authors: Tanaka Hiroki , Ishida Kyoko , Ozawa Kenji , Ishihara Takuma , Sawada Akira , Mochizuki Kiyofumi , Yamamoto Tetsuya
Published: October 2020

Background:

The nasal to temporal amplitudes ratio (N/T) of multifocal electroretinography (mfERG) scans measured within 5° of the macula can be used to detect glaucomatous change. The photopic negative response (PhNR) of mfERG elicited by a circular stimulus centered on the fovea was significantly reduced in eyes with glaucoma. The PhNR to B-wave ratio (PhNR/B) is the optimal measure of the PhNR. However, clinical superiority for evaluating glaucoma patients has not been determined between N/T and PhNR/B yet.

Methods: For morphological assessments, ganglion cell complex (GCC) in six regions and the average were measured by optical coherence tomography (OCT). For functional assessment, Humphrey visual fields (VF) with mean sensitivities (MT) and mfERG scans with parameters of N/T and the multifocal photopic negative response to B-wave ratio (mfPhNR/B) were measured. Sixty-nine eyes of forty-four glaucoma patients were included and correlations between mfERG parameters and OCT or VF parameters were evaluated.

Results: The mean age of patients was 59.4 years. The mean deviation for all eyes obtained with the VF 30-2 and VF 10-2 was -7.00 and -6.31 dB, respectively. Significant correlations between GCC thickness or VF parameter and the N/T were found, especially in the inferior and inforotemporal retinal areas corresponding to superior and superonasal VF sectors (GCC vs N/T; coefficient = -7.916 and -7.857, and MT vs N/T ; coefficient = -4.302 and -4.437, in the inferior and inforotemporal retinal areas, respectively, all p values < 0.05). However, similar associations were not obtained between mfPhNR/B and OCT or VF parameters. The mfPhNR/B only in the inferotemporal sector was significantly correlated with the average thickness of GCC (coefficient = 4.823, P = 0.012).

Conclusions: The N/T is better parameter than PhNR/B for the assessment of glaucomatous eyes in the current study’s measurement conditions. A future study modifying the stimuli and amplitudes to obtain the spatial correspondence to OCT and VF measurement will be required to evaluate the value of mfERG.
Bloodstream Infections caused by Klebsiella pneumoniae and Serratia marcescens isolates co-harboring NDM-1 and KPC-2.
Authors: Bes Taniela , Nagano Debora , Martins Roberta , Marchi Ana Paula , Neto Lauro Perdigão , Higashino Hermes , Prado Gladys , Guimaraes Thais , Levin Anna S , Costa Silvia
Published: October 2020

Carbapenem-resistant Enterobacteriaceae are a worldwide health problem and isolates carrying both blaKPC-2 and blaNDM-1 are unusual. Here we describe the microbiological and clinical characteristics of five cases of bloodstream infections (BSI) caused by carbapenem-resistant Klebsiella pneumoniae and Serratia marcescens having both blaKPC-2 and blaNDM-1. Of the five blood samples, three are from hematopoietic stem cell transplantation patients, one from a renal transplant patient, and one from a soft tissue surgical patient. All patients lived in low-income neighbourhoods and had no travel history. Despite antibiotic treatment, four of five patients died. The phenotypic susceptibility assays showed that meropenem added either EDTA, phenylboronic acid (PBA) or both, increased the zone of inhibition in comparison to meropenem alone. Molecular tests showed the presence of blaKPC-2 and blaNDM-1 genes. K. pneumoniae isolates were assigned to ST258 or ST340 by whole genome sequencing. This case-series showed a high mortality among patients with BSI caused by Enterobacteriae harbouring both carbapenemases. The detection of carbapenemase-producing isolates carrying both blaKPC-2 and blaNDM-1 remains a challenge when using only phenotypic assays. Microbiology laboratories must be alert for K. pneumoniae isolates producing both KPC-2 and NDM-1.
Luteolin Inhibits Respiratory Syncytial Virus Replication by Regulating the MiR-155/SOCS1/STAT1 Signaling Pathway
Authors: Wang Saisai , Ling Yiting , Yao Yuanyuan , Zheng Gang , Chen Wenbin
Published: October 2020

Background: Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory tract infection in infants, children, immunocompromised adults, and elderly individuals. Currently, there are few therapeutic options available to prevent RSV infection. The present study aimed to investigate the effects of luteolin on RSV replication and the related mechanisms. Material and methods: We pretreated cells and mice with luteolin before infection with RSV, the virus titer, expressions of RSV-F, interferon (IFN)-stimulated genes (ISGs), and production of IFN-α and IFN-β were determined by plaque assay, RT-qPCR, and ELISA, respectively. The activation of Janus kinase (JAK)-signal transducer and activator of transcription 1 (STAT1) signaling pathway was detected by Western blotting and luciferase assay. Proteins which negatively regulates STAT1 was determined by Western blotting. Then cells were transfected with suppressor of cytokine signaling 1 (SOCS1) plasmid and virus replication and ISGs expression was determined. Luciferase reporter assay and Western blotting was performed to detect the relationship between SOCS1 and miR-155. Results: Luteolin inhibited RSV replication, as shown by the decreased viral titer and RSV-F mRNA expression both in vitro and in vivo. The antiviral activity of luteolin was attributed to the enhanced phosphorylation of STAT1, resulting in the increased production of ISGs. Further study showed that SOCS1 was downregulated by luteolin and SOCS1 is a direct target of microRNA-155 (miR-155). Inhibition of miR-155 rescued luteolin-mediated SOCS1 downregulation, whereas upregulation of miR-155 enhanced the inhibitory effect of luteolin. Conclusion: Luteolin inhibits RSV replication by regulating the miR-155/SOCS1/STAT1 signaling pathway.
Effect of capsular tension ring implantation on capsular stability after phacoemulsification in patients with weak zonules:a randomized controlled trail.
Authors: Yang Shangfei , Jiang Hui , Nie Kailai , Feng Liwen , Fan Wei
Published: October 2020

Background: Patients who had previously undergone pars plana vitrectomy (PPV) or who had severe myopia may have weaker zonules, for which a capsular tension ring (CTR) implantation is still controversial. This study is to test the effect of CTR implantation on capsular stability after phacoemulsification in those patients. Methods: A total of 42 patients who underwent phacoemulsification and received an intraocular lens (IOL) were randomized to undergo CTR implantation or not. The primary outcomes were uncorrected distant visual acuity (UDVA), best corrected distant visual acuity (BCDVA), refractive prediction error, the area of anterior capsulorhexis, and IOL inclination angle. Follow-up visits were conducted postoperatively at 1 day, 1 week, 1 month and 3 months. Outcomes were compared between all CTR and control patients, as well as between subgroups who had previously undergone PPV or who had severe myopia. Results: Surgery significantly improved UDVA and BCDVA to similar extents in CTR and control patients, and refraction prediction error was similar between the two groups at all follow-up times. At 3 months after surgery, the area of anterior capsulorhexis was significantly larger in CTR patients than in controls (p = 0.0199). These differences were also significant between the subgroups of patients with strong myopia. Vertical IOL inclination was less within CTR groups at 3 months after surgery, especially in patients with severe myopia(p=0.0286). At 1 week postoperatively, the proportion of individuals whose posterior lens capsule that had completely adhered to the posterior IOL surface was significantly higher among CTR patients (p = 0.023). No serious surgical complications were observed. Conclusion: CTR implantation can benefit cataract patients with weak zonules by maintaining the shape of the capsular bag, reducing capsule shrinkage and stabilizing IOL inclination.
Protective CD8+ T-cell response against Hantaan virus infection induced by immunization with designed linear multi-epitope peptides in HLA-A2.1/Kb transgenic mice
Authors: Ma Ying , Tang Kang , Zhang Yusi , Zhang Chunmei , Cheng Linfeng , Zhang Fanglin , Zhuang Ran , Jin Boquan , Zhang Yun
Published: October 2020

Background: An effective vaccine that prevents disease caused by hantaviruses is a global public health priority, but up to now, no vaccine has been approved for worldwide use. Therefore, novel vaccines with high prophylaxis efficacy are urgently needed.Methods: Herein, we designed and synthesized Hantaan virus (HTNV) linear multi-epitope peptide consisting of HLA-A*02-restricted HTNV cytotoxic T cell (CTL) epitope and pan HLA-DR-binding epitope (PADRE), and evaluated the immunogenicity, as well as effectiveness, of multi-epitope peptides in HLA-A2.1/Kb transgenic mice with interferon (IFN)-γ enzyme-linked immunospot assay, cytotoxic mediator detection, proliferation assay and HTNV-challenge test.Results: The results showed that a much higher frequency of specific IFN-γ-secreting CTLs, high levels of granzyme B production, and a strong proliferation capacity of specific CTLs were observed in splenocytes of mice immunized with multi-epitope peptide than in those of a single CTL epitope. Moreover, pre-immunization of multi-epitope peptide could reduce the levels of HTNV RNA loads in the liver, spleen and kidneys of mice, indicating that specific CTL responses induced by multi-epitope peptide could reduce HTNV RNA loads in vivo.Conclusions: This study may provide an important foundation for the development of novel peptide vaccines for HTNV prophylaxis.
OphthatomeTM: An integrated knowledgebase of ophthalmic diseases for translating vision research into the clinic
Authors: Raj Praveen , Tejwani Sushma , Sudha Dandayudhapani , B Muthu Narayanan , Thangapandi Chandrasekar , Das Sankar , J Somasekar , Mangalapudi Susmithasane , Kumar Durgesh , Pindipappanahalli Narendra , Shetty Rohit , Ghosh Arkashubra , Kumaramanickavel Govindasamy , Chaudhuri Amitabha , Nagaswamy Soumittra
Published: October 2020

Background: Medical big data analytics has revolutionized the human healthcare system by introducing processes that facilitate rationale clinical decision making, predictive or prognostic modelling of disease progression and management, disease surveillance, impact on public health and research. Although, the electronic medical records (EMR) system is the digital storehouse of rich medical data of a large patient cohort collected over many years, the data lack sufficient structure to be of clinical value for applying deep learning methods and advanced analytics to improve disease management at an individual patient level or for the discipline in general. Ophthatome TM captures data contained in retrospective electronic medical records between September 2012 and January 2018 to facilitate translational vision research through a knowledgebase of eye diseases. Methods: The electronic medical records data from Narayana Nethralaya recorded in the MS-SQL database was mapped and programmatically transferred to MySQL. The captured data was manually curated to preserve data integrity and accuracy. The data is stored in MySQL database management system for ease of visualization, advanced search functions and other knowledgebase applications. Results: Ophthatome TM is a comprehensive and accurate knowledgebase of ophthalmic diseases containing curated clinical, treatment and imaging data of 581,466 ophthalmic cases from the Indian population, recorded between September 2012 and January 2018. Ophthatome TM provides filters and Boolean searches with operators and modifiers that allow selection of specific cohorts covering 524 distinct ophthalmic disease types and 1800 disease sub-types across 35 different anatomical regions of the eye. The availability of longitudinal data for about 300,000 subjects provides additional opportunity to perform clinical research on disease progression and management including drug response and treatment outcome. The knowledgebase captures eye diseases in a genetically diverse population providing opportunity to study genetic and environmental factors contributing to or influencing eye diseases. Conclusion: Ophthatome TM will accelerate clinical, genomic, pharmacogenomic and advanced translational research on ophthalmic diseases.
Lifespan Regulation by Insulin Signaling Through Phosphorylation of Proteins Beyond FOXO
Authors: Li Wen-jun , Tao Li , wang Chenwei , Yan Yong-Hong , Zhang Mei-Jun , Liu Ze-Xian , Li Yu-Xin , Zhao Hanqing , Li Xue-Mei , He Xian-Dong , Xue Yu , Dong Meng-Qiu
Published: October 2020

Insulin/IGF-1 Signaling (IIS) constrains longevity by inhibiting the transcription factor FOXO. Beyond FOXO, little is known about how phosphorylation—as mediated by IIS kinases— regulates lifespan. Here, we profiled IIS-dependent phosphorylation changes in a large-scale quantitative phosphoproteomic analysis of wild-type and three IIS mutant C. elegans strains. Our state-of-the-art analysis experimentally identified more than 15,000 phosphosites, among which 448 were differentially phosphorylated in the long-lived daf-2/insulin receptor mutant. We developed a machine-learning-based tool for systematically ranking the likely functional importance of phosphosites to guide candidate selection for follow-up validation. We show that AKT-1 pT492 inhibits DAF-16/FOXO and compensates the loss of daf-2 function, that EIF-2α pS49 potently regulates protein synthesis and daf-2 longevity, and that reduced phosphorylation of multiple germline proteins (e.g., CDK-1) apparently transmits a signal representing reduced DAF-2 signaling to the soma. Finally, kinase-substrate analysis and subsequent experimental validation confirm that casein kinase 2 negatively regulates lifespan. Our new benchmark data resource and machine-learning tool enables unprecedented access to detailed functional insights for studies of longevity.
Diversity of SCCmec Elements in Methicillin-Resistant Staphylococcus Aureus (MRSA) Recovered from a Healthy Student Population in Kenya
Authors: Khasabuli Osborn , Ngugi Caroline , Kiiru John
Published: October 2020

Background: Methicillin-resistant S. aureus continues to be a concern for public health systems, particularly due to infections emerging in non-hospital settings. Resistance to methicillin is presently classified as a serious phenomenon because the majority of methicillin-resistant strains are also multi-drug resistant. The genetic determinant of resistance to methicillin and other β-lactam antibiotics is the mec-A gene, which lies in the SCCmec resistance island. In Kenya, studies done previously have shown the existence of SCCmec types in clinical isolates, but similar information on isolates recovered from healthy populations is scanty.Methods: A cross-sectional study was conducted on healthy university students residing within the university residence halls to determine the carriage of MRSA. MRSA was detected using Cefoxitin (30µg), and mec-A gene and Sccmec elements were detected using conventional PCR methods. A total of 237 students were recruited, and 657 swabs were collected using standards methods for recovering S. aureus.Results: A total of 231 S. aureus isolates were recovered, out of which 26 (11.3%) were MRSA. Out of the 26 MRSA strains, 17 carried the mecA gene in their gene cassettes. SCCmecV was the most prevalent (61.5%), followed by SCCmecII (53.9%) among the MRSA strains. SCCmecIVa, SCCmecIVb, SCCmecIVc and SCCmecIVd were absent in all the isolates. SCCmecV was found to be highly prevalent (64.7%) followed by SCCmecII, 8 (47.1%) among the mecA-positive MRSA strains. On the other hand, small proportions of mecA-negative isolates harbored SCCmecI (0.9%), SCCmecII (3.3%), SCCmec III (0.5%) and SCCmecV (2.3%).Conclusion: This study revealed that the strains recovered from the student population were highly diverse in terms of the SCCmec elements they carried in their gene cassettes.
ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1
Authors: Zhang Huafeng , Zhang Tong , Sun Linchong , Hao Yijie , Ma Wenhao , Shen Shengqi , Suo Caixia , Zhang Pinggen , Wang Ting , Li Shi-Ting , Chen Zhaolin , Wei Haoran , Yan Ronghui , Zhang Yi , Cai Yongping , Jia Weidong , Huang Fang , Gao Ping
Published: October 2020

α-Enolase 1 (ENO1) is a critical glycolytic enzyme whose aberrant expression drives the pathogenesis of various cancers. ENO1 has been indicated to have additional roles beyond its conventional metabolic activity, but the underlying mechanisms and biological consequences remain elusive. Here, we show that ENO1 suppresses iron regulatory protein 1 (IRP1) expression to regulate iron homeostasis and survival of hepatocellular carcinoma (HCC) cells. Mechanistically, we unprecedentedly uncover that ENO1, as an RNA-binding protein, recruits CNOT6 to accelerate the mRNA decay of IRP1 in cancer cells, leading to inhibition of mtioferin-1 (Mfrn1) expression and subsequent repression of mitochondrial iron-induced ferroptosis. Moreover, through in vitro and in vivo experiments and clinical sample analysis, we identified IRP1 and Mfrn1 as tumor suppressors by inducing ferroptosis in HCC cells. Taken together, this study establishes a novel role for the ENO1/IRP1/Mfrn1 pathway in the pathogenesis of HCC and reveals a previously unknown connection between the ENO1/IRP1/Mfrn1 pathway and ferroptosis, suggesting a potential innovative cancer therapy.
Community Mental Health Care In Mexico: A Look At The State Of Jalisco, Mexico
Authors: Carmona-Huerta Jaime , Cardona-Muller David , Durand-Arias Sol , Allen Rodriguez , Guarner-Catalá Carmen , Eduardo Madrigal-de León , Alvarado Ruben
Published: October 2020

Background: Access to mental health care is a worldwide public health challenge. In Mexico an unacceptably high percentage of the population with mental disorders does not receive the necessary treatment, which is mainly due to the lack of access to mental health care. The community mental health care model was created and has been implemented to improve this situation. In order to properly plan and implement this model a precise situational diagnosis of the mental health care network is required, thus this is a first approach to evaluate the community mental health networks in the state of Jalisco. Methods: Two components from the EvaRedCom–TMS instrument were used including a general description and accessibility of the community mental health care network. A geographic and economic accessibility evaluation was carried out for the different regions of the state ranging from scattered rural to urban communities using information gathered from health institutions, telephone interviews and computer applications. Results: Jalisco’s community mental health network includes a total of 31 centers and 0.64 mental health workers for every 10,000 inhabitants >15 years of age. The mean transportation cost required to access mental health care was 16.25 USD. The time needed to reach the closest mental health center in 7 of the 13 analyzed regions was more than 30 minutes and the mean time required to reach a prolonged stay center was 172.7 minutes with transportation cost of 22.3 USD. Some marginalized regions in the state have a mean 114 minutes required to reach the closest mental health care center and 386 minutes to reach a prolonged stay center. Conclusions: This first approach to evaluate the mental health networks in Mexico showed that there are multiple barriers to access its care including an unfavorable number of human resources, long distances and high costs. The identification of Jalisco’s mental health network deficiencies is the first step towards establishing a properly planned community mental health care model within the country.
A review of the TOPHAT (Turned-down Onto Pericapsular-tissue Hemisectioned Amputated Toe) filleted toe flap technique for wound coverage during ray amputations
Authors: Sim Nadia , Lee Shaun , Yap Hao Yun , Tan Qian Ying , Tan Jerilyn , Wong Daniel , Chau Amanda , Mak Manfred , Chong Tze Tec , Tay Hsien Ts’ung
Published: October 2020

Background: Wound healing post ray amputation for toe gangrene is commonly complicated by adjacent digital loss after applying Topical Negative Pressure Wound Therapy (TNPWT). This is either due to mechanical soft tissue erosion or trauma to the adjacent digital artery from direct pressure effect. This ultimately results in exposure of the adjacent Metatarsal-Phalangeal Joint (MTPJ) with eventual gangrene and a larger wound bed, thus mandating further ray amputation and subsequently enlarge the wound bed. We describe the use of the TOPHAT filleted toe flap technique – a combination of a filleted toe flap to protect the adjacent MTPJ capsule and a novel Negative Pressure Wound Therapy with instillation and dwell-time (NPWTi-d) dressing technique. The fillet flap protects the adjacent joint capsule and reduce the wound burden whilst allowing the wound to benefit from TNPWT, thereby accelerating wound healing. Methods: We conducted a prospective review of patients with toe gangrene requiring ray amputations who underwent the TOPHAT filleted toe flap technique in our institution from 2019 and 2020. Complications such as wound dehiscence, hematoma, flap necrosis and secondary infection were recorded. Other outcomes recorded were time taken to final skin grafting and time taken for complete wound epithelialisation. Results: 9 patients underwent treatment with the TOPHAT filleted toe flap technique between 2019 and 2020. 2 patients had minimal tip necrosis of the flap which required minor debridement. All except 2 patients whom declined further treatment, progressed to definitive skin coverage with skin grafting. One patient had progressive arterial disease despite successful skin grafting and required above knee amputation. The mean time to final skin grafting was 49.5 days post-operatively and the mean time to final wound epithelialisation was 107.5 days post-operatively. Patients were all satisfied with the outcomes and were able to return to pre-morbid function. Conclusions: The TOPHAT filleted toe flap technique has a consistent vascular supply that provides durable soft tissue coverage. It is a robust and easily reproducible technique to accelerate wound healing after ray amputations even in patients with peripheral vascular disease.