bluuebunny/crossref_metadata_2025 huggingface dataset:
Developing an integrated framework of healthcare leaders’ resilience
Authors: Förster Charlotte , Duchek Stephanie , Geithner Silke , Krägler Maxie
Published: August 2022

Healthcare institutions have been under pressure for years now, climaxing in the COVID-19 crisis. Even if they are not operating at a trouble spot of the current COVID-19 pandemic, healthcare leaders need to be highly resilient to remain effective as well as staying healthy themselves. The purpose of this study is to shed light on healthcare leaders’ challenges and, more important, their specific resilience factors. By doing so, we can expand the knowledge of this fragmented research field and deliver an integrated framework that has so far been lacking. We conducted 20 in-depth interviews with healthcare leaders. More precisely, 35% (7) of our interviewees were physicians, 55% (11) worked in nursing, and 10% (2) were nonclinical professionals, with 50% (10) of our participants working in upper, 40% (8) in middle, and 10% (2) in lower leadership positions. Based on a qualitative content analysis approach, we identified main healthcare leaders’ challenges as well as crucial resilience factors (i.e., individual, situational, and behavioral factors). By integrating insights from contemporary leadership and work-related resilience research, we were able to develop an integrated framework of healthcare leaders’ resilience. Considering resilience as a context-dependent construct, we are contributing to the resilience and healthcare literature by investigating the specialty of healthcare leaders’ resilience. This study is contributing to the future development of resilience interventions in healthcare organizations that might help not only healthcare leaders to better cope with critical situations but also promote resilience development among their followers and organizations.
Comparison Between the Video Head Impulse Test and Caloric Irrigation During Acute Vertigo
Authors: Olivecrona Elin , Zborayova Katarina , Barrenäs Marie-Louise , Salzer Jonatan
Published: August 2022

Caloric irrigation (CI) is the gold standard to investigate peripheral vestibular dysfunction. The video head impulse test (vHIT) is faster and more accessible and may be useful during acute vertigo stroke risk differentiation. Comparative studies between the two methods are needed. The objective of this study was to compare vestibular function data derived from caloric irrigation with that from vHIT. This study included 80 patients with acute onset vertigo who underwent caloric irrigation and vHIT. CI derived sum of slow phase velocities (SPVs) and unilateral weakness (UW) were compared with vHIT vestibulo-ocular reflex (VOR) gain and gain asymmetry (GA) using correlation analyses. Optimal cut offs for vHIT VOR gain and GA were calculated using Youden indexes. There was a strong positive correlation between the asymmetry measures UW and GA whereas the correlation between the sum of SPVs and VOR gain was weaker. The optimal cut offs to diagnose unilateral vestibular weakness were 0.80 for VOR gain and 28% for GA; with specificities for predicting normal caloric irrigation results of 55% and 93%, respectively. In one third of cases the results from caloric irrigation and vHIT dissociated. The results from vHIT correlated with those from CI, still neither test seem to have the accuracy to replace the other. GA appears as an attractive measure in acute vertigo as the high specificity can be used to identify those with a substantial probability of normal vestibular function in need of more comprehensive work-up for central causes. To diagnose vestibular dysfunction, CI remains gold standard.
Diagnostic performance of metagenomic next-generation sequencing in non-tuberculous mycobacterial pulmonary disease when applied to clinical practice
Authors: Wei Wei , Cao Jie , Wu Xiao-cui , Cheng Li-ping , Shen Xiao-na , Sha Wei , Sun Qin
Published: August 2022

Objective

To compare non-tuberculous mycobacterial pulmonary disease (NTMPD) diagnosis by metagenomic next-generation sequencing (mNGS) with Bactec mycobacterial growth indicator tube (MGIT) 960.

Methods

A total of 422 patients with suspected NTMPD in Shanghai Pulmonary Hospital between January 2020 and May 2021 were retrospectively analyzed; 194 were diagnosed with NTMPD. The diagnostic performance of mNGS and MGIT 960 for NTMPD was assessed. Receiver operating characteristic (ROC) curves and areas under curve (AUCs) were compared.

Results

The sensitivity of mNGS in NTMPD diagnosis was 81.4% and higher than that of MGIT 960 (53.6%). The specificity of mNGS in NTMPD diagnosis was 97.8%, similar to that of MGIT 960 (100%). The sensitivity of combined mNGS and MGIT 960 in NTMPD diagnosis was 91.8%. The sensitivity of mNGS for bronchoalveolar lavage fluid (BALF), pulmonary puncture tissue fluid, and sputum was 84.8%, 80.6%, and 77.5%, respectively; all were higher than that of MGIT 960 (P < 0.05). The AUC of mNGS and MGIT 960 was 0.897 and 0.768, respectively. The AUC of mNGS were BALF (0.916), pulmonary puncture tissue fluid (0.903), and sputum (0.870).

Conclusion

The sensitivity of mNGS was superior to that of Bactec MGIT 960; the specificity in NTMPD diagnosis was similar. mNGS shows effective performance in NTMPD diagnosis.
Identity Development in Disorientating Times: the Experiences of Medical Students During COVID-19
Authors: Brown Megan E. L. , Lim Jun Hua , Horsburgh Jo , Pistoll Chance , Thakerar Viral , Maini Arti , Johnson Caroline , Beaton Laura , Mahoney Claire , Kumar Sonia
Published: August 2022

Introduction

Professional identity development is a central aim of medical education, which has been disrupted during COVID-19. Yet, no research has qualitatively explored COVID-19’s impact across institutions or countries on medical students’ identities. Kegan proposes a cognitive model of identity development, where ‘disorientating dilemmas’ prompt student development. Given the potential of COVID-related disruption to generate disorientating dilemmas, the authors investigated the ways in which COVID-19 influenced students’ identity development.

Methods

The authors conducted an international qualitative study with second year medical students from Imperial College London, and third year students from Melbourne Medical School. Six focus groups occurred 2020–2021, with three to six students per group. Authors analysed data using reflexive thematic analysis, applying Kegan’s model as a sensitising theoretical lens.

Results

COVID-19 has resulted in a loss of clinical exposure, loss of professional relationships, and a shift in public perception of physicians. Loss of exposure to clinical practice removed the external validation from patients and seniors many students depended on for identity development. Students’ experiences encouraged them to assume the responsibilities of the profession and the communities they served, in the face of conflicting demands and risk. Acknowledging and actioning this responsibility facilitated identity development as a socially responsible advocate.

Conclusions

Educators should consider adapting medical education to support students through Kegan’s stages of development. Measures to foster relationships between students, patients, and staff are likely necessary. Formal curricula provisions, such as spaces for reflection and opportunities for social responsibility, may aid students in resolving the conflict many have recently experienced.
A deterministic and nature-inspired algorithm for the fuzzy multi-objective path optimization problem
Authors: Ma Yi-Ming , Hu Xiao-Bing , Zhou Hang
Published: August 2022

Increasing evaluation indexes have been involved in the network modeling, and some parameters cannot be described precisely. Fuzzy set theory becomes a promising mathematical method to characterize such uncertain parameters. This study investigates the fuzzy multi-objective path optimization problem (FMOPOP), in which each arc has multiple crisp and fuzzy weights simultaneously. Fuzzy weights are characterized by triangular fuzzy numbers or trapezoidal fuzzy numbers. We adopt two fuzzy number ranking methods based on their fuzzy graded mean values and distances from the fuzzy minimum number. Motivated by the ripple spreading patterns on the natural water surface, we propose a novel ripple-spreading algorithm (RSA) to solve the FMOPOP. Theoretical analyses prove that the RSA can find all Pareto optimal paths from the source node to all other nodes within a single run. Numerical examples and comparative experiments demonstrate the efficiency and robustness of the newly proposed RSA. Moreover, in the first numerical example, the processes of the RSA are illustrated using metaphor-based language and ripple spreading phenomena to be more comprehensible. To the best of our knowledge, the RSA is the first algorithm for the FMOPOP that can adopt various fuzzy numbers and ranking methods while maintaining optimality.
An enhanced whale optimization algorithm with improved dynamic opposite learning and adaptive inertia weight strategy
Authors: Cao Di , Xu Yunlang , Yang Zhile , Dong He , Li Xiaoping
Published: August 2022

Whale Optimization Algorithm (WOA), as a newly proposed swarm-based algorithm, has gradually become a popular approach for optimization problems in various engineering fields. However, WOA suffers from the poor balance of exploration and exploitation, and premature convergence. In this paper, a new enhanced WOA (EWOA), which adopts an improved dynamic opposite learning (IDOL) and an adaptive encircling prey stage, is proposed to overcome the problems. IDOL plays an important role in the initialization part and the algorithm iterative process of EWOA. By evaluating the optimal solution in the current population, IDOL can adaptively switch exploitation/exploration modes constructed by the DOL strategy and a modified search strategy, respectively. On the other hand, for the encircling prey stage of EWOA in the latter part of the iteration, an adaptive inertia weight strategy is introduced into this stage to adaptively adjust the prey’s position to avoid falling into local optima. Numerical experiments, with unimodal, multimodal, hybrid and composition benchmarks, and three typical engineering problems are utilized to evaluate the performance of EWOA. The proposed EWOA also evaluates against canonical WOA, three sub-variants of EWOA, three other common algorithms, three advanced algorithms and four advanced variants of WOA. Results indicate that according to Wilcoxon rank sum test and Friedman test, EWOA has balanced exploration and exploitation ability in coping with global optimization, and it has obvious advantages when compared with other state-of-the-art algorithms.
Representation Learning in Multi-view Clustering: A Literature Review
Authors: Chen Man-Sheng , Lin Jia-Qi , Li Xiang-Long , Liu Bao-Yu , Wang Chang-Dong , Huang Dong , Lai Jian-Huang
Published: August 2022

Multi-view clustering (MVC) has attracted more and more attention in the recent few years by making full use of complementary and consensus information between multiple views to cluster objects into different partitions. Although there have been two existing works for MVC survey, neither of them jointly takes the recent popular deep learning-based methods into consideration. Therefore, in this paper, we conduct a comprehensive survey of MVC from the perspective of representation learning. It covers a quantity of multi-view clustering methods including the deep learning-based models, providing a novel taxonomy of the MVC algorithms. Furthermore, the representation learning-based MVC methods can be mainly divided into two categories, i.e., shallow representation learning-based MVC and deep representation learning-based MVC, where the deep learning-based models are capable of handling more complex data structure as well as showing better expression. In the shallow category, according to the means of representation learning, we further split it into two groups, i.e., multi-view graph clustering and multi-view subspace clustering. To be more comprehensive, basic research materials of MVC are provided for readers, containing introductions of the commonly used multi-view datasets with the download link and the open source code library. In the end, some open problems are pointed out for further investigation and development.
Persistence
Authors: Miller Kristie
Published: August 2022

Persistence realism is the view that ordinary sentences that we think and utter about persisting objects are often true. Persistence realism involves both a semantic claim, about what it would take for those sentences to be true, and an ontological claim about the way things are. According to persistence realism, given what it would take for persistence sentences to be true, and given the ontology of our world, often such sentences are true. According to persistence error-theory, they are not. This Element considers several different views about the conditions under which those sentences are true. It argues for a view on which it is relatively easy to vindicate persistence realism, because all it takes is for the world to be the way it seems to us. Thereby it argues for the view that relations of numerical identity, or of being-part-of-the-same-object, are neither necessary nor sufficient for persistence realism.
The Distributive Politics of Environmental Protection in Latin America and the Caribbean
Authors: Alcañiz Isabella , Gutiérrez Ricardo A.
Published: August 2022

The study of environmental politics in Latin America and the Caribbean expands as conflicts stemming from the deterioration of the natural world increase. Yet this scholarship has not generated a broad research agenda similar to the ones that emerged around other key political phenomena. This Element seeks to address the lack of a comprehensive research agenda in Latin American and Caribbean environmental politics and helps integrate the existing, disparate literatures. Drawing from distributive politics, this Element asks who benefits from the appropriation and pollution of the environment, who pays the costs of climate change and environmental degradation, and who gains from the allocation of state protections.
From insights to action: Enriching the clinical and translational research workforce by developing diverse and inclusive career programs
Authors: Ruiz Raquel , Vitale Alfred , Schwartz Ann , Ossip Deborah , Zand Martin S. , Dozier Ann , Egede Leonard E.
Published: August 2022

Diversification of the Translational Science workforce is a strategic goal for the National Center for the Advancement of Translational Science (NCATS) program. NCATS has identified the development of translational science education, training, and support for a diverse translational science workforce as key to advancing the growing field of translational science. An annual mixed-methods assessment has been conducted on Common Metrics data submitted by over 60 Clinical & Translational Science Awards (CTSA) programs nationwide and includes metrics addressing recruitment and retention of scientists with particular attention to underrepresented persons and women. This article describes a methodology for the development of From Insights to Action, a resource for guiding program implementation and strategic planning to develop a diverse clinical and translational science workforce. This was informed by the Common Metrics Initiative process and constituted of findings from qualitative interviews of a subset of CTSAs that participated. The dissemination of this guide had several impacts, including providing structural foci for the CTSA Fall 2020 program meeting centered on Diversity, Equity, and Inclusion in translational science; addressing NCATS’ goal of workforce diversity; and understanding the number of diverse graduates still engaged in research.
Research 101: An online course introducing medical students to research
Authors: Blackard Jason T. , Knapke Jacqueline M. , Ryan Patrick H. , Schuckman Stephanie , Veevers Jennifer , Hardie William D. , Heubi James E.
Published: August 2022

Introduction:

Research is an important aspect of many medical students’ training. However, many medical students are not required to complete a scholarly project, and formal research training is often fragmented across the medical school curriculum. Thus, we developed an online, structured, asynchronous set of modules to introduce trainees to multiple topics relevant to the conduct of research.

Methods:

Research 101 was piloted by 27 first-year medical students at the University of Cincinnati College of Medicine. Students’ knowledge, confidence, and satisfaction were assessed using a final quiz and pre- and post-module surveys with five-point Likert-scaled questions and open-ended text responses.

Results:

Pre-module survey results showed that learners felt most confident in Conducting a literature search and least confident in Submitting an Institutional Review Board (IRB) protocol at UC. Post-module mean scores were significantly increased compared to pre-module results for all modules and questions (P < 0.05). The response to “The content of this module met my needs” was high across all modules with 236 (84.0%) “yes” responses. Thematic analysis of open-ended text responses from post-module surveys identified several improvements to individual modules and to the overall structure of Research 101. A final quiz of 25 multiple choice questions covering content from all required modules was required. The median score was 21.

Conclusions:

Comparison of post-module to pre-module survey scores provided clear evidence of improved learning across all topics. The modules developed were responsive to the students’ needs, and students provided additional improvements for subsequent iterations of Research 101.
Quasi-adelic measures and equidistribution on
Authors: MAVRAKI NIKI MYRTO , YE HEXI
Published: August 2022

Baker and Rumely, Favre, Rivera, and Letelier, and Chambert-Loir proved an important arithmetic equidistribution theorem for points of small height associated to an adelic measure. To broaden the scope in which arithmetic equidistribution may be employed, we generalize the notion of an adelic measure to that of a quasi-adelic measure and show that arithmetic equidistribution holds for quasi-adelic measures as well. We exhibit examples of non-adelic, quasi-adelic measures arising from the dynamics of quadratic rational maps. In fact, we show that the measures that arise in applications of arithmetic equidistribution theorems are typically not adelic. Finally, we motivate our definition of a quasi-adelic measure by relating it to a seemingly different problem in arithmetic dynamics arising from results of Call, Tate, and Silverman in the study of abelian varieties.
Indaziflam reduces downy brome (Bromus tectorum) density and cover five years after treatment in sagebrush-grasslands with no impact on perennial grass cover
Authors: Courkamp Jacob S. , Meiman Paul J. , Nissen Scott J.
Published: August 2022

The invasive annual grass downy brome (Bromus tectorumL.) is a critical threat to the semiarid shrublands that characterize western North America. More abundant fine fuel after invasion typically increases fire frequency in plant communities adapted to relatively infrequent burning, reducing the likelihood of native plant persistence. Currently, imazapic is most often used to manageB. tectorum, but reinvasion from the seedbank after treatment is common. Indaziflam is a newer herbicide recently labeled for use in rangelands grazed by livestock, and many research trials have demonstrated its ability to deplete invasive annual grass seedbanks. We evaluated the effectiveness of indaziflam and imazapic for reducingB. tectorumdensity and cover over a period of approximately 5 yr (57 mo after treatment [MAT]) at two invaded sagebrush-grassland sites near Pinedale, WY. Treatments included three different indaziflam rates (51, 73, and 102 g ai ha−1) and one imazapic rate (123 g ai ha−1), and these treatments were reapplied to half of each plot at 45 MAT to evaluate the effects of two sequential applications. We also measured perennial grass cover, because positive perennial grass responses were observed after release fromB. tectorumcompetition in other studies, and perennial grasses may provide resistance toB. tectorumreinvasion. Intermediate and high indaziflam rates (73 and 102 g ha−1, respectively) reducedB. tectorumcover and density at 45 MAT, and perennial grass cover responded positively to some treatments, mostly early in the study (≤33 MAT). Imazapic reducedB. tectoruminitially, but did not affect density or cover at either site beyond 21 MAT. Reapplication did not substantially improveB. tectorumcontrol at 57 MAT in plots treated with intermediate and high indaziflam rates, suggesting that long-term control with a single indaziflam treatment may be possible in some cases.
A randomized experiment evaluating survey mode effects for video interviewing
Authors: Endres Kyle , Hillygus D. Sunshine , DeBell Matthew , Iyengar Shanto
Published: August 2022

Rising costs and challenges of in-person interviewing have prompted major surveys to consider moving online and conducting live web-based video interviews. In this paper, we evaluate video mode effects using a two-wave experimental design in which respondents were randomized to either an interviewer-administered video or interviewer-administered in-person survey wave after completing a self-administered online survey wave. This design permits testing of both within- and between-subject differences across survey modes. Our findings suggest that video interviewing is more comparable to in-person interviewing than online interviewing across multiple measures of satisficing, social desirability, and respondent satisfaction.
Topcross pearl millet hybrids for Africa: A case study for Mali
Authors: Sanogo Moussa D. , Coulibaly S. B. , Mariko Moussa , Witcombe John R.
Published: August 2022

Single-cross pearl millet hybrids are widely grown in India, but this cultivar type has not been adopted in Africa. Hybrids from India have proven to be highly susceptible to downy mildew disease in Africa, the continent where the disease originated. We investigated an alternative strategy of growing topcross hybrids where both the parents are of African origin and both are only partially inbred. We investigated root characteristics – length, diameter, area, volume and dry weight – under drought stress and well-watered controlled conditions in genotypes that included potential parents of topcross hybrids. Several, including population Civarex 06_05, had better developed roots under drought while also having good roots under more favourable conditions. Some of these genotypes were backrossed to a male-sterile line based on the A4 cytoplasm to produce male-sterile populations with stable male sterility. Civarex 06_05 had good combining ability when used as a female parent and produced high-yielding topcross hybrids. Two of the topcross hybrids were on restorer populations Toroniou, originating from Mali, and Maiwa from Nigeria. On the experimental station and in farmers’ fields, one was much superior in yield to the best local alternatives and both had superior downy mildew resistance. Current efforts on the seed production of these topcross hybrids are described, and the constraints to the commercialisation in Mali of promising topcross hybrids are discussed.
Common Men, Exceptional Politicians: What Do We Gain from an Embodied Social Biographical Approach to Leftist Leaders Like Germany's August Bebel and Brazil's Luis Inácio Lula da Silva?
Authors: French John D.
Published: August 2022

Lula and His Politics of Cunning explores the origin, roots, and evolution of Luis Inácio Lula da Silva's vision, discourse, and practice of leadership as a process of becoming. This commentary invites historians of labor movements and the left to think beyond their geographical and chronological specializations. It argues that there is much to gain from thinking globally if we wish to achieve meaningful causal insights applicable to the sweep of capitalist development.
Pursuing the Individual in Working-Class History
Authors: Barrett James R.
Published: August 2022

The individual is still rare in working-class history, and, when we find them, they are often, like Bebel and Lula, exceptional. We are interested in them as leaders of vital mass movements and because they had an important impact on their societies. But another part of the promise of biographies like these is the opportunity to approach the personal dimensions of working-class experience through an individual life. Bringing the two biographies together highlights the diversity of working-class experience. Bebel developed in a racially homogeneous society while Lula was a mixed-race person shaped in race-conscious Brazil. Bebel thrived as a small-shop artisan while Lula thrived as a skilled worker in a mass production factory. I also compare and contrast these two subjects with two American labor radicals, the socialist leader Eugene Debs and William Z. Foster, a key figure in the Communist Party of the US. The importance of individual psychology and the homosocial worlds of these subjects might have played a greater role here, while the ubiquitous learning of both men raises the problem of working-class intellectual history, another subject that has not received enough attention from labor historians.
Executive function as a generalized determinant of psychopathology and functional outcome in school-aged autism spectrum disorder: a case-control study
Authors: Wong Oscar W. H. , Barzilay Ran , Lam Angela M. W. , Chan Sandra , Calkins Monica E. , Gur Raquel E. , Gur Ruben C.
Published: August 2022

Background

Individuals with autism spectrum disorder (ASD) are challenged not only by the defining features of social-communication deficits and restricted repetitive behaviors, but also by a myriad of psychopathology varying in severity. Different cognitive deficits underpin these psychopathologies, which could be subjected to intervention to alter the course of the disorder. Understanding domain-specific mediating effects of cognition is essential for developing targeted intervention strategies. However, the high degree of inter-correlation among different cognitive functions hinders elucidation of individual effects.

Methods

In the Philadelphia Neurodevelopmental Cohort, 218 individuals with ASD were matched with 872 non-ASD controls on sex, age, race, and socioeconomic status. Participants of this cohort were deeply and broadly phenotyped on neurocognitive abilities and dimensional psychopathology. Using structural equation modeling, inter-correlation among cognitive domains were adjusted before mediation analysis on outcomes of multi-domain psychopathology and functional level.

Results

While social cognition, complex cognition, and memory each had a unique pattern of mediating effect on psychopathology domains in ASD, none had significant effects on the functional level. In contrast, executive function was the only cognitive domain that exerted a generalized negative impact on every psychopathology domain (p factor, anxious-misery, psychosis, fear, and externalizing), as well as functional level.

Conclusions

Executive function has a unique association with the severity of comorbid psychopathology in ASD, and could be a target of interventions. As executive dysfunction occurs variably in ASD, our result also supports the clinical utility of assessing executive function for prognostic purposes.
Who Should Represent Future Generations in Climate Planning?
Authors: Byskov Morten Fibieger , Hyams Keith
Published: August 2022

Extreme impacts from climate change are already being felt around the world. The policy choices that we make now will affect not only how high global temperatures rise but also how well-equipped future economies and infrastructures are to cope with these changes. The interests of future generations must therefore be central to climate policy and practice. This raises the questions:Whoshould represent the interests of future generations with respect to climate change? And according to whichcriteriashould we judge whether a particular candidate would make an appropriate representative for future generations? In this essay, we argue that potential representatives of future generations should satisfy what we call a “hypothetical acceptance criterion,” which requires that the representative could reasonably be expected to be accepted by future generations. This overarching criterion in turn gives rise to two derivative criteria. These are, first, the representative'sepistemic and experiential similarity to future generations, and second, his or hermotivation to act on behalf of future generations. We conclude that communities already adversely affected by climate change best satisfy these criteria and are therefore able to command the hypothetical acceptance of future generations.
Communities and Climate Change: Why Practices and Practitioners Matter
Authors: Grix Marco , Watene Krushil
Published: August 2022

Communities most vulnerable to the effects of climate change, such as reduced access to material resources and increased exposure to adverse weather conditions, are intimately tied to a considerable amount of cultural and biological diversity on our planet. Much of that diversity is bound up in the social practices of Indigenous groups, which is why these practices have great long-term value. Yet, little attention has been given to them by philosophers. Also neglected have been the historical conditions and contemporary realities that constrain these practices and devalue the knowledge of their practitioners. In this essay, we make the case for preserving a diverse range of social practices worldwide, and we argue that this is possible only by strengthening the communities of practitioners who enact them in the contexts in which they are adaptive. By concentrating on Indigenous communities, we show how focusing on practices can transform how Indigenous and other local communities are represented in global climate-change conversations and policy as a matter of justice. More specifically, we argue that practice-centered thinking and local practices provide critical insights for determining the extent to which climate policies protect and enable transformative change.
A neonate with crossed pulmonary arteries: a case report and literature review of 115 cases worldwide
Authors: Alhassan Abdalrahman A. , Shati Ayed A. , Al-Ahmari Sami M. , Elhadam Afia E. , Bushari Tajudeen M. , Elhag Omer H. , Elhoury Motea E.
Published: August 2022

Malposition of the branch pulmonary arteries (MBPA) is an unusual malformation characterised by anomalous origin of both pulmonary arteries from the main pulmonary trunk. To date, only few cases have been reported. Herein, we present the first case report of a 3-day-old, full-term male neonate with the lesser form of crossed pulmonary arteries in Saudi Arabia detected by echocardiography and confirmed by cardiac CT. Crossed pulmonary arteries is not a rare anomaly, but it is a somewhat underreported anomaly, and their recognition is important because it is usually associated with other CHDs, airway obstruction, extra-cardiac anomalies, and certain genetic syndromes.
Speaking Machines, the Trial of Articulation, and Deaf Education in Modern France
Authors: Arnaud Sabine
Published: August 2022

If cochlear implants continue to meet with much resistance from parts of the Deaf community and beyond, this reflects constructions of speech that have been at the core of conceptions of humankind for over three centuries. Starting in the 1750s, Julien Offray de La Mettrie advocated for deaf people's potential for speech. This was also the time of the creation of schools for deaf children, which led to a surge of debate about teaching sign language versus speech. The reception of the speaking machine of Canon Mical, a now forgotten inventor, offered another context in which to question the source of the expressive power of language. By retracing debates about the mechanical nature of articulated speech, the potential limits of communication, and what really constitutes its expressive power, we can better understand how the experience of current technology develops out of conflicts first introduced at the birth of modernity.
Changes in physical activity and adiposity with all-cause, cardiovascular disease, and cancer mortality
Authors: Ahmadi Matthew N. , Lee I-Min , Hamer Mark , del Pozo Cruz Borja , Chen Li Jung , Eroglu Elif , Lai Yun-Ju , Ku Po Wen , Stamatakis Emmanuel
Published: August 2022

Background

The relationship between joint changes in physical activity and adiposity with mortality is not well understood. We examined the association of changes in these two established risk factors with all-cause (ACM), cardiovascular disease (CVD), and cancer mortality.

Methods

We used longitudinal data from Taiwan’s MJ Cohort, comprising 116,228 general population adults recruited from 1998-2013 with repeated measures 4.6 y (2.5) apart and followed up for mortality for 11.9 y (3.5). Physical activity, body mass index (BMI), waist circumference (WC), and body fat percentage (BF%) groups and changes were based on public health and clinical guidelines.

Results

Compared to stable-insufficient physical activity, increasing physical activity from any baseline level was associated with lower ACM (HR [95%CI]): 0.85 [0.74, 0.96]) and CVD mortality (0.72 [0.55, 0.93]) risk. This was approximately equal to meeting physical activity guidelines at both timepoints (eg: 0.71 [0.58, 0.88] for CVD mortality). Compared to stable-overweight/moderate adiposity, decreasing adiposity level attenuated but did not offset mortality risk for all three outcomes (eg: BMI = 0.95 [0.76, 1.16] for CVD mortality). Only maintaining a healthy adiposity level at both timepoints offset mortality risk (BMI = 0.75 [0.61, 0.89]) for CVD mortality). In the joint changes analyses, lower mortality risk was a consequence of increases in physical activity across adiposity change groups (eg: WC decrease = 0.57 [0.48, 0.67]; WC stability = 0.73 [0.66, 0.80], WC increase = 0.83 [0.72, 0.97] for ACM). Decreasing adiposity attenuated the negative associations of decreased physical activity (BF% = 1.13 [0.95, 1.35] for ACM).

Conclusions

We found a lower risk for ACM, CVD, and cancer mortality from increasing physical activity and an attenuation from decreasing adiposity regardless of baseline levels. The beneficial associations of joint changes were primarily driven by physical activity, suggesting lower mortality risk may be more immediate through physical activity improvements compared to adiposity improvements alone.
Chemotaxis may assist marine heterotrophic bacterial diazotrophs to find microzones suitable for N2 fixation in the pelagic ocean
Authors: Hallstrøm Søren , Raina Jean-Baptiste , Ostrowski Martin , Parks Donovan H , Tyson Gene W , Hugenholtz Philip , Stocker Roman , Seymour Justin R , Riemann Lasse
Published: August 2022

Heterotrophic bacterial diazotrophs (HBDs) are ubiquitous in the pelagic ocean, where they have been predicted to carry out the anaerobic process of nitrogen fixation within low-oxygen microenvironments associated with marine pelagic particles. However, the mechanisms enabling particle colonization by HBDs are unknown. We hypothesized that HBDs use chemotaxis to locate and colonize suitable microenvironments, and showed that a cultivated marine HBD is chemotactic toward amino acids and phytoplankton-derived DOM. Using an in situ chemotaxis assay, we also discovered that diverse HBDs at a coastal site are motile and chemotactic toward DOM from various phytoplankton taxa and, indeed, that the proportion of diazotrophs was up to seven times higher among the motile fraction of the bacterial community compared to the bulk seawater community. Finally, three of four HBD isolates and 16 of 17 HBD metagenome assembled genomes, recovered from major ocean basins and locations along the Australian coast, each encoded >85% of proteins affiliated with the bacterial chemotaxis pathway. These results document the widespread capacity for chemotaxis in diverse and globally relevant marine HBDs. We suggest that HBDs could use chemotaxis to seek out and colonize low-oxygen microenvironments suitable for nitrogen fixation, such as those formed on marine particles. Chemotaxis in HBDs could therefore affect marine nitrogen and carbon biogeochemistry by facilitating nitrogen fixation within otherwise oxic waters, while also altering particle degradation and the efficiency of the biological pump.
Nucleosome remodeling and deacetylation complex and MBD3 influence mouse embryonic stem cell naïve pluripotency under inhibition of protein kinase C
Authors: Dai Yujian , Sun Jialei , He Na , An Liyou , Hou Chunhui , Du Fuliang
Published: August 2022

The pluripotency of naïve mouse embryonic stem cells (mES) is regulated by multiple signaling pathways, with inhibition of protein kinase C (PKCi) playing a particularly important role in maintaining naïve mES. However, the regulatory function of nucleosome remodeling and deacetylase (NuRD) complex in mES cultured in a PKCi system is unknown. We found that, compared with 2iL-derived mES, PKCi-derived mES showed low mRNA expression of NuRD complex subunits, including MBD3, HDAC1/HDAC2, MTA1, and RbAP46/RbAP48. Western blot showed that PKCi-derived mES expressed lower protein levels of MBD3 and HDAC2 at passage 3, as well as MBD3, HDAC2, and MTA1 at passage 10, indicating that PKCi suppressed NuRD complex expression. Knockdown of MBD3 increased PKCi-derived mES pluripotency by increasing NANOG and OCT4 expression and colony formation. By contrast, overexpression of MBD3 or removal of PKC inhibitor-induced differentiation of mES, results in reduced NANOG, OCT4, and REX1 expression and colony formation, increased differentiation-related gene expression, and differentiation into flat cells. Knockdown of MBD3 in mES upon PKC inhibitor removal partially reversed cell differentiation. Our results show that the regulatory NuRD complex and its MBD3 subunit influence the naïve pluripotency of mES cultured in a PKCi system.
Coupled influence of tectonics, climate, and surface processes on landscape evolution in southwestern North America
Authors: Bahadori Alireza , Holt William E. , Feng Ran , Austermann Jacqueline , Loughney Katharine M. , Salles Tristan , Moresi Louis , Beucher Romain , Lu Neng , Flesch Lucy M. , Calvelage Christopher M. , Rasbury E. Troy , Davis Daniel M. , Potochnik Andre R. , Ward W. Bruce , Hatton Kevin , Haq Saad S. B. , Smiley Tara M. , Wooton Kathleen M. , Badgley Catherine
Published: August 2022

The Cenozoic landscape evolution in southwestern North America is ascribed to crustal isostasy, dynamic topography, or lithosphere tectonics, but their relative contributions remain controversial. Here we reconstruct landscape history since the late Eocene by investigating the interplay between mantle convection, lithosphere dynamics, climate, and surface processes using fully coupled four-dimensional numerical models. Our quantified depth-dependent strain rate and stress history within the lithosphere, under the influence of gravitational collapse and sub-lithospheric mantle flow, show that high gravitational potential energy of a mountain chain relative to a lower Colorado Plateau can explain extension directions and stress magnitudes in the belt of metamorphic core complexes during topographic collapse. Profound lithospheric weakening through heating and partial melting, following slab rollback, promoted this extensional collapse. Landscape evolution guided northeast drainage onto the Colorado Plateau during the late Eocene-late Oligocene, south-southwest drainage reversal during the late Oligocene-middle Miocene, and southwest drainage following the late Miocene.
Real-time 3D analysis during electron tomography using tomviz
Authors: Schwartz Jonathan , Harris Chris , Pietryga Jacob , Zheng Huihuo , Kumar Prashant , Visheratina Anastasiia , Kotov Nicholas A. , Major Brianna , Avery Patrick , Ercius Peter , Ayachit Utkarsh , Geveci Berk , Muller David A. , Genova Alessandro , Jiang Yi , Hanwell Marcus , Hovden Robert
Published: August 2022

The demand for high-throughput electron tomography is rapidly increasing in biological and material sciences. However, this 3D imaging technique is computationally bottlenecked by alignment and reconstruction which runs from hours to days. We demonstrate real-time tomography with dynamic 3D tomographic visualization to enable rapid interpretation of specimen structure immediately as data is collected on an electron microscope. Using geometrically complex chiral nanoparticles, we show volumetric interpretation can begin in less than 10 minutes and a high-quality tomogram is available within 30 minutes. Real-time tomography is integrated into tomviz, an open-source and cross-platform 3D data analysis tool that contains intuitive graphical user interfaces (GUI), to enable any scientist to characterize biological and material structure in 3D.
Frequency wavelength multiplexed optoacoustic tomography
Authors: Stylogiannis Antonios , Prade Ludwig , Glasl Sarah , Mustafa Qutaiba , Zakian Christian , Ntziachristos Vasilis
Published: August 2022

Optoacoustics (OA) is overwhelmingly implemented in the Time Domain (TD) to achieve high signal-to-noise ratios by maximizing the excitation light energy transient. Implementations in the Frequency Domain (FD) have been proposed, but suffer from low signal-to-noise ratios and have not offered competitive advantages over time domain methods to reach high dissemination. It is therefore commonly believed that TD is the optimal way to perform optoacoustics. Here we introduce an optoacoustic concept based on pulse train illumination and frequency domain multiplexing and theoretically demonstrate the superior merits of the approach compared to the time domain. Then, using recent advances in laser diode illumination, we launch Frequency Wavelength Multiplexing Optoacoustic Tomography (FWMOT), at multiple wavelengths, and experimentally showcase how FWMOT optimizes the signal-to-noise ratios of spectral measurements over time-domain methods in phantoms and in vivo. We further find that FWMOT offers the fastest multi-spectral operation ever demonstrated in optoacoustics.
Highly conserved shifts in ubiquitin-proteasome system (UPS) activity drive mitochondrial remodeling during quiescence
Authors: Yue Sibiao , Wang Lei , DeMartino George N. , Zhao FangZhou , Liu Yi , Sieber Matthew H.
Published: August 2022

Defects in cellular proteostasis and mitochondrial function drive many aspects of infertility, cancer, and other age-related diseases. All of these conditions rely on quiescent cells, such as oocytes and adult stem cells, that reduce their activity and remain dormant as part of their roles in tissue homeostasis, reproduction, and even cancer recurrence. Using a multi-organism approach, we show that dynamic shifts in the ubiquitin proteasome system drive mitochondrial remodeling during cellular quiescence. In contrast to the commonly held view that the ubiquitin-proteasome system (UPS) is primarily regulated by substrate ubiquitination, we find that increasing proteasome number and their recruitment to mitochondria support mitochondrial respiratory quiescence (MRQ). GSK3 triggers proteasome recruitment to the mitochondria by phosphorylating outer membrane proteins, such as VDAC, and suppressing mitochondrial fatty acid oxidation. This work defines a process that couples dynamic regulation of UPS activity to coordinated shifts in mitochondrial metabolism in fungi, Drosophila, and mammals during quiescence.
Molecular and in vivo studies of a glutamate-class prolyl-endopeptidase for coeliac disease therapy
Authors: del Amo-Maestro Laura , Mendes Soraia R. , Rodríguez-Banqueri Arturo , Garzon-Flores Laura , Girbal Marina , Rodríguez-Lagunas María José , Guevara Tibisay , Franch Àngels , Pérez-Cano Francisco J. , Eckhard Ulrich , Gomis-Rüth F. Xavier
Published: August 2022

The digestion of gluten generates toxic peptides, among which a highly immunogenic proline-rich 33-mer from wheat α-gliadin, that trigger coeliac disease. Neprosin from the pitcher plant is a reported prolyl endopeptidase. Here, we produce recombinant neprosin and its mutants, and find that full-length neprosin is a zymogen, which is self-activated at gastric pH by the release of an all-β pro-domain via a pH-switch mechanism featuring a lysine plug. The catalytic domain is an atypical 7+8-stranded β-sandwich with an extended active-site cleft containing an unprecedented pair of catalytic glutamates. Neprosin efficiently degrades both gliadin and the 33-mer in vitro under gastric conditions and is reversibly inactivated at pH > 5. Moreover, co-administration of gliadin and the neprosin zymogen at the ratio 500:1 reduces the abundance of the 33-mer in the small intestine of mice by up to 90%. Neprosin therefore founds a family of eukaryotic glutamate endopeptidases that fulfils requisites for a therapeutic glutenase.
ROR2 regulates self-renewal and maintenance of hair follicle stem cells
Authors: Veltri Anthony , Lang Christopher M. R. , Cangiotti Gaia , Chan Chim Kei , Lien Wen-Hui
Published: August 2022

Hair follicles undergo cycles of regeneration fueled by hair follicle stem cells (HFSCs). While β-catenin-dependent canonical Wnt signaling has been extensively studied and implicated in HFSC activation and fate determination, very little is known about the function of β-catenin-independent Wnt signaling in HFSCs. In this study, we investigate the functional role of ROR2, a Wnt receptor, in HFSCs. By analyzing Ror2-depleted HFSCs, we uncover that ROR2 is not only essential to regulate Wnt-activated signaling that is responsible for HFSC activation and self-renewal, but it is also required to maintain proper ATM/ATR-dependent DNA damage response, which is indispensable for the long-term maintenance of HFSCs. In analyzing HFSCs lacking β-catenin, we identify a compensatory role of ROR2-PKC signaling in protecting β-catenin-null HFSCs from the loss of stem cell pool. Collectively, our study unveils a previously unrecognized role of ROR2 in regulation of stem cell self-renewal and maintenance.
Bacterial biofilm functionalization through Bap amyloid engineering
Authors: Matilla-Cuenca Leticia , Taglialegna Agustina , Gil Carmen , Toledo-Arana Alejandro , Lasa Iñigo , Valle Jaione
Published: August 2022

Biofilm engineering has emerged as a controllable way to fabricate living structures with programmable functionalities. The amyloidogenic proteins comprising the biofilms can be engineered to create self-assembling extracellular functionalized surfaces. In this regard, facultative amyloids, which play a dual role in biofilm formation by acting as adhesins in their native conformation and as matrix scaffolds when they polymerize into amyloid-like fibrillar structures, are interesting candidates. Here, we report the use of the facultative amyloid-like Bap protein ofStaphylococcus aureusas a tool to decorate the extracellular biofilm matrix or the bacterial cell surface with a battery of functional domains or proteins. We demonstrate that the localization of the functional tags can be change by simply modulating the pH of the medium. Using Bap features, we build a tool for trapping and covalent immobilizing molecules at bacterial cell surface or at the biofilm matrix based on the SpyTag/SpyCatcher system. Finally, we show that the cell wall of several Gram-positive bacteria could be functionalized through the external addition of the recombinant engineered Bap-amyloid domain. Overall, this work shows a simple and modulable system for biofilm functionalization based on the facultative protein Bap.