bluuebunny/crossref_metadata_2025 huggingface dataset:
Composite Bonding Pre-Treatment with Laser Radiation of 3 µm Wavelength: Comparison with Conventional Laser Sources
Authors: Blass David , Nyga Sebastian , Jungbluth Bernd , Hoffmann Hans-Dieter , Dilger Klaus
Published: July 2018

To use the full potential of composite parts, e.g., to reduce the structural weight of cars or airplanes, a greater focus is needed on the joining technology. Adhesive bonding is considered favorable, superior joining technology for these parts. Unfortunately, to provide a structural and durable bond, a surface pre-treatment is necessary. Due to its high integration potential in industrial process chains, laser radiation can be a very efficient tool for this purpose. Within the BMBF-funded (German Federal Ministry of Education and Research) project GEWOL, a laser source that emits radiation at 3 µm wavelength (which shows significant advantages in theory) was developed for a sensitive laser-based bonding pre-treatment. Within the presented study, the developed laser source was compared with conventional laser sources emitting radiation at 355 nm, 1064 nm, and 10,600 nm in terms of application for a composite bonding pre-treatment. With the different laser sources, composites were treated, analytically tested, subsequently bonded, and mechanically tested to determine the bonding ability of the treated specimens. The results show a sensitive treatment of the surface with the developed laser source, which resulted in a very effective cleaning, high bonding strengths (over 32 MPa), and a good effectiveness compared with the conventional laser sources.
Mechanical Modelling of the Plastic Flow Machining Process
Authors: Vu Viet Q. , Beygelzimer Yan , Kulagin Roman , Toth Laszlo S.
Published: July 2018

A new severe plastic deformation process, plastic flow machining (PFM), was introduced recently to produce sheet materials with ultrafine and gradient structures from bulk samples in one single deformation step. During the PFM process, a part of a rectangular sample is transformed into a thin sheet or fin under high hydrostatic pressure. The obtained fin is heavily deformed and presents a strain gradient across its thickness. The present paper aims to provide better understanding about this new process via analytical modelling accompanied by finite element simulations. PFM experiments were carried out on square commercially pure aluminum (CP Al) billets. Under pressing, the material flowed from the horizontal channel into a narrow 90° oriented lateral channel to form a fin sheet product, and the remaining part of the sample continued to move along the horizontal channel. At the opposite end of the bulk sample, a back-pressure was applied to increase the hydrostatic pressure in the material. The experiments were set at different width sizes of the lateral channel under two conditions; with or without applying back-pressure. A factor called the lateral extrusion ratio was defined as the ratio between the volume of the produced fin and the incoming volume. This ratio characterizes the efficiency of the PFM process. The experimental results showed that this ratio was greater when back-pressure was applied and further, it increased with the rise of the lateral channel width size. Finite element simulations were conducted in the same boundary conditions as the experiments using DEFORM-2D/3D software, V11.0. Two analytical models were also established. The first one used the variational principle to predict the lateral extrusion ratio belonging to the minimum total plastic power. The second one employed an upper-bound approach on a kinematically admissible velocity field to describe the deformation gradient in the fin. The numerical simulations and the analytical modelling successfully predicted the experimental tendencies, including the deformation gradient across the fin thickness.
Impact of intensive and traditional rehabilitation on quadriceps strength after anterior cruciate ligament reconstructive surgery
Authors: Streckis Vytautas , Skurvydas Albertas , Zachovajevas Pavelas , Gudas Rimtautas , Lukšaitė Justė , Trumpickas Vytenis
Published: July 2018

After knee anterior cruciate ligament reconstructive surgery, the recovery of the former level of physical activity takes from 3 to 12 months. Such a wide range of recovery period of physical activity suggests that rehabilitation in most cases is not optimal. According to the majority of authors, after the surgery, a patient can resume intensive physical activity, when the difference in muscle strength between the operated lower extremity and another extremity is not greater than 10–15%. The aim of this study was to compare the impact of intensive and normal rehabilitations on the recovery of knee extensor muscle strength after the surgery. Material and methods. A total of 40 patients were enrolled in this study. The subjects were divided into two groups. Both groups were engaged in physical activity. The mean age of patients (16 men and 4 women) in the first group at the time of surgery was 26.4±8.1 years, mean height – 179.8±8.5 cm, and mean weight – 76.0±14.0 kg. An intensive rehabilitation was applied for the first group of the patients studied. The second group consisted of 13 men and 7 women who were engaged in moderate physical activity. Their mean age at the time of surgery was 27.0±9.3 years, mean height – 173.2±6.2 cm, and mean weight – 71.0±9.0 kg. A traditional rehabilitation was applied to this group. Muscle strength was measured in the patients of both groups studied approximately 5.2 months following surgery using the Biodex isokinetic dynamometer. Results. The patients undergoing an intensive rehabilitation achieved higher levels of knee extensor muscle strength than those patients undergoing a traditional rehabilitation program. Applying an aggressive rehabilitation program, knee extensor muscles recover more quickly than using a traditional rehabilitation program. The comparison of intensive and traditional rehabilitation programs applied to the operated and unoperated lower extremities has shown that the indexes of knee extensor muscle strength differed by 11.51– 12.74%. Applying a traditional rehabilitation, a 23.68–49.42% difference in knee flexor muscle strength between operated and unoperated extremities was noted. Conclusions. The effect of intensive rehabilitation aimed at strength recovery of knee extensor muscles after anterior cruciate ligament reconstructive surgery is greater than after ordinary rehabilitation.
How well informed are pharmacy customers in Estonia about minor illnesses and over-the-counter medicines
Authors: Volmer Daisy , Lilja John , Hamilton David
Published: July 2018

Objective. To determine how knowledgeable pharmacy customers are regarding their own health and information about minor illnesses and over-the-counter medicines. Methods. A written questionnaire was sent in the period of January to April 2003 to a sample of pharmacies in different regions of Estonia. Questionnaires were then distributed to randomly selected pharmacy customers. Of 886 questionnaires given out, 727 were answered and returned; a response rate was 82%. Results. Half of the respondents assessed their health as good or excellent. When minor illnesses occurred, most used home remedies or over-the-counter medicines. Their knowledge of such illnesses was better than that of over-the-counter medicines. In both cases, the pharmacist was the most frequently used information source. Drug administration, which preparation should be chosen for which illness, and the drug price were the three most often asked questions in the pharmacy. The information about over-the-counter medicines given in the pharmacies was regarded as sufficient by 51% of the respondents. Conclusion. Although lay people do know about over-the-counter medicines and especially about minor illnesses, pharmacists play a very important part in providing information about medicines. However, drug communication by healthcare professionals regarding over-the-counter medicines should be given more attention in the future.
Influenza virus
Authors: Juozapaitis Mindaugas , Antoniukas Linas
Published: July 2018

Every year, especially during the cold season, many people catch an acute respiratory disease, namely flu. It is easy to catch this disease; therefore, it spreads very rapidly and often becomes an epidemic or a global pandemic. Airway inflammation and other body ailments, which form in a very short period, torment the patient several weeks. After that, the symptoms of the disease usually disappear as quickly as they emerged. The great epidemics of flu have rather unique characteristics; therefore, it is possible to identify descriptions of such epidemics in historic sources. Already in the 4th century BC, Hippocrates himself wrote about one of them. It is known now that flu epidemics emerge rather frequently, but there are no regular intervals between those events. The epidemics can differ in their consequences, but usually they cause an increased mortality of elderly people. The great flu epidemics of the last century took millions of human lives. In 1918–19, during “The Spanish” pandemic of flu, there were around 40–50 millions of deaths all over the world; “Pandemic of Asia” in 1957 took up to one million lives, etc. Influenza virus can cause various disorders of the respiratory system: from mild inflammations of upper airways to acute pneumonia that finally results in the patient’s death. Scientist Richard E. Shope, who investigated swine flu in 1920, had a suspicion that the cause of this disease might be a virus. Already in 1933, scientists from the National Institute for Medical Research in London – Wilson Smith, Sir Christopher Andrewes, and Sir Patrick Laidlaw – for the first time isolated the virus, which caused human flu. Then scientific community started the exhaustive research of influenza virus, and the great interest in this virus and its unique features is still active even today.
Successful rescue therapy with mycophenolate mofetil in kidney transplantation improves the long-term graft survival
Authors: Kahu Jaanus , Lõhmus Aleksander , Ilmoja Madis , Kirsimägi Ülle , Timberg Gennadi , Peetsalu Ants
Published: July 2018

Objective. The aim of this study was to compare the graft survival after kidney transplantation in patients treated with azathioprine (AZA) or mycophenolate mofetil (MMF) and analyze the significance of different risk factors for graft survival. Material and methods. A total of 137 patients, transplanted between January 1996 and June 2001, were retrospectively divided into two groups: patients who received AZA together with cyclosporine A and methylprednisolone (AZA group, n=72) and patients who received MMF either immediately or were switched from AZA to MMF during 3 months (MMF group, n=65). Results. According to Kaplan-Meier analysis, a 1-year graft survival was 79% in the AZA group and 85% in the MMF group; a 6-year graft survival was 51% and 67%, respectively (P=0.046). Multivariate Cox survival model demonstrated that MMF therapy reduced the risk of graft loss by 34% (P=0.028), while delayed graft function increased the risk of graft loss (risk ratio 2.26, P=0.009). A statistically significant difference in total cholesterol level (6.7 vs. 5.7 mmol/L, respectively; P=0.002), mean systolic blood pressure (145 vs. 134 mmHg, P=0.009), and cyclosporine A daily dose (238 vs. 203 mg, P=0.015) between the AZA and MMF groups at 1 year was revealed. Conclusion. MMF rescue therapy improves the long-term graft survival compared to AZA despite high early rejection rate and avoids the negative impact of acute rejections on graft survival.
Bioactive Compounds and Extracts from Traditional Herbs and Their Potential Anti-Inflammatory Health Effects
Authors: Serrano Antonio , Ros Gaspar , Nieto Gema
Published: July 2018

The inflammatory processes associated with several chronic illnesses like cardiovascular disease and cancer have been the focus of mechanistic studies of the pathogenicity of these diseases and of the use of different pharmacological and natural methods to prevent them. In this study we review the current evidence regarding the effectiveness of natural extracts from as-yet little-studied traditional botanical species in alleviating the inflammation process associated with several chronic diseases. Additionally, the intention is to expose the known pathways of action and the potential synergistic effects of the constituent compounds of the discussed extracts. It is noted that the here-studied extracts, which include black garlic rich in S-allylcystein, polyphenols from cat’s claw (Uncaria tomentosa), devil’s claw (Harpagophytum procumbens), camu-camu (Myrciaria dubia), and blackcurrant (Ribes nigrum), and citrus fruit extracts rich in hesperidin, have similar or greater effects than other, more extensively studied extracts such as tea and cocoa. The combined use of all of these extracts can give rise to synergetic effects with greater biological relevance at lower doses.
Graphene-Based Semiconductor Heterostructures for Photodetectors
Authors: Shin Dong , Choi Suk-Ho
Published: July 2018

Graphene transparent conductive electrodes are highly attractive for photodetector (PD) applications due to their excellent electrical and optical properties. The emergence of graphene/semiconductor hybrid heterostructures provides a platform useful for fabricating high-performance optoelectronic devices, thereby overcoming the inherent limitations of graphene. Here, we review the studies of PDs based on graphene/semiconductor hybrid heterostructures, including device physics/design, performance, and process technologies for the optimization of PDs. In the last section, existing technologies and future challenges for PD applications of graphene/semiconductor hybrid heterostructures are discussed.
Quantitative 3D Association of Geological Factors and Geophysical Fields with Mineralization and Its Significance for Ore Prediction: An Example from Anqing Orefield, China
Authors: Qin Yaozu , Liu Liangming
Published: July 2018

Quantitative 3D spatial association of geological factors and geophysical fields with orebodies is critical for ore prediction. The Anqing orefield, a principal Cu–Fe orefield in China, is closely associated with the Yueshan intrusion. By compiling the data from drilling and tunnelling exploration, Controlled Source Audiofrequency Magnetotelluric (CSAMT) surveying and the computational modelling of magmatic intrusion’s cooling process, we constructed models of the Yueshan intrusion, ore-favourable carbonate formation, orebodies, resistivity field and volume strain field. These models are used as evidential factors to analyse their spatial association with mineralization by the weights-of-evidence (WofE) method. The location of orebodies is closely related to the shape of the contact zone of the Yueshan intrusion. The spaces with the distance ≤200 m to the concaves that were selected by minimum principal curvature (|Kmin| ≥ 0.0025) from contact zones, are very favourable for localization of orebodies. Most orebodies are not located in the spaces of the lowest resistivity, suggesting that the lowest resistivity cannot be used as an indicator for mineralization. The spaces with higher positive volumetric strain have higher positive weights with orebodies, implying that the mineralization is positively related to the positive volumetric strain. The spaces of all evidential factors that had positive correlation with mineralization were integrated to create a 3D prospectivity map by calculating posterior probability. Five areas with higher posterior probability, indicating higher prospectivity potential, are selected as targets for future exploration.
Carbon Material with High Specific Surface Area Improves Complex Copper Ores’ Bioleaching Efficiency by Mixed Moderate Thermophiles
Authors: Hao Xiaodong , Liu Xueduan , Zhu Ping , Chen Aijia , Liu Hongwei , Yin Huaqun , Qiu Guanzhou , Liang Yili
Published: July 2018

The catalysis of carbon materials with different specific surface areas (SSA) (2, 400, 800 and 1200 m2/g) on complex copper ores bioleaching by moderately mixed thermophiles was investigated. The copper extractions increased with the rise in SSA of carbon materials. A recovery of 98.8% copper in the presence of 1200 m2/g activated carbon was achieved, and improved by 30.7% and 76.4% compared with biotic control and chemical leaching. Moreover, the addition of 1200 m2/g activated carbon adsorbed large amount of bacteria, accelerated the oxidation rate of ferrous iron and maintained the solution redox potential at relatively low values, and significantly increased the dissolution of primary copper sulfide (62.7%) compared to biotic control (6.0%). Microbial community succession revealed that activated carbon changed the microbial community composition dramatically. The S. thermosulfidooxidans ST strain gained a competitive advantage and dominated the microbial community through the whole bioleaching process. The promoting effect of carbon material with higher SSA on copper extraction was mainly attributed to better galvanic interaction, biofilm formation, direct contact and lower redox potential.
The Biological and Biophysical Properties of the Spider Peptide Gomesin
Authors: Tanner John D. , Deplazes Evelyne , Mancera Ricardo L.
Published: July 2018

This review summarises the current knowledge of Gomesin (Gm), an 18-residue long, cationic anti-microbial peptide originally isolated from the haemocytes of the Brazilian tarantula Acanthoscurria gomesiana. The peptide shows potent cytotoxic activity against clinically relevant microbes including Gram-positive and Gram-negative bacteria, fungi, and parasites. In addition, Gm shows in-vitro and in-vivo anti-cancer activities against several human and murine cancers. The peptide exerts its cytotoxic activity by permeabilising cell membranes, but the underlying molecular mechanism of action is still unclear. Due to its potential as a therapeutic agent, the structure and membrane-binding properties, as well as the leakage and cytotoxic activities of Gm have been studied using a range of techniques. This review provides a summary of these studies, with a particular focus on biophysical characterisation studies of peptide variants that have attempted to establish a structure-activity relationship. Future studies are still needed to rationalise the binding affinity and cell-type-specific selectivity of Gm and its variants, while more pre-clinical studies are required to develop Gm into a therapeutically useful peptide.
Phytotoxic Activity of Metabolites Isolated from Rutstroemia sp.n., the Causal Agent of Bleach Blonde Syndrome on Cheatgrass (Bromus tectorum)
Authors: Masi Marco , Meyer Susan , Górecki Marcin , Pescitelli Gennaro , Clement Suzette , Cimmino Alessio , Evidente Antonio
Published: July 2018

A fungal pathogen soon to be described as Rutstroemia capillus-albis (Rutstroemiaceae, Helotiales, Leotiomycetes) has been identified as the causal agent of ‘bleach blonde syndrome’ on the invasive annual grass weed Bromus tectorum (cheatgrass) in western North America. This apparently common but previously undescribed disease causes premature senescence and sterility, but does not affect seed germination or seedling emergence and growth. This study investigated whether the new species produces phytotoxins that could be implicated in pathogenesis. The compounds 9-O-methylfusarubin, 9-O-methylbostrycoidin, 5-O-methylnectriafurone, trans-methyl-p-coumarate and terpestacin were isolated from the solid culture of this fungus. The undescribed absolute stereochemistry at C-3 of 9-O-methylfusarubin and at C-1’ of 5-O-methylnectriafurone were assigned by applying electronic and vibrational circular dichroism (ECD and VCD) combined with computational methods and the advanced Mosher’s method, respectively. The first three listed compounds are naphtoquinone pigments, while terpestacin is a sesterterpene, and trans-methyl-p-coumarate could be the product of an unusual fungal phenylpropanoid biosynthesis pathway. In a juvenile plant immersion bioassay, both 9-O-methylfusarubin and terpestacin proved to be highly toxic at 10−4 M, causing wilting and plant death within 10 days. This finding suggests that these two compounds could play a role in pathogenesis on B. tectorum.
Dual THz Wave and X-ray Generation from a Water Film under Femtosecond Laser Excitation
Authors: Huang Hsin-hui , Nagashima Takeshi , Hsu Wei-hung , Juodkazis Saulius , Hatanaka Koji
Published: July 2018

Simultaneous emission of the THz wave and hard X-ray from thin water free-flow was induced by the irradiation of tightly-focused femtosecond laser pulses (35 fs, 800 nm, 500 Hz) in air. Intensity measurements of the THz wave and X-ray were carried out at the same time with time-domain spectroscopy (TDS) based on electro-optic sampling with a ZnTe(110) crystal and a Geiger counter, respectively. Intensity profiles of the THz wave and X-ray emission as a function of the solution flow position along the incident laser axis at the laser focus show that the profile width of the THz wave is broader than that of the X-ray. Furthermore, the profiles of the THz wave measured in reflection and transmission directions show different features and indicate that THz wave emission is, under single-pulse excitation, induced mainly in laser-induced plasma on the water flow surface. Under double-pulse excitation with a time separation of 4.6 ns, 5–10 times enhancements of THz wave emission were observed. Such dual light sources can be used to characterise materials, as well as to reveal the sequence of material modifications under intense laser pulses.
Enhanced Visible Light Driven Photocatalytic Behavior of BiFeO3/Reduced Graphene Oxide Composites
Authors: Si Yun-hui , Xia Yu , Shang Shao-ke , Xiong Xin-bo , Zeng Xie-rong , Zhou Ji , Li Ya-yun
Published: July 2018

BiFeO3/Reduced Graphene Oxide (BFO/RGO) composites have been fabricated by a simple hydrothermal method. The X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman, and X-ray photoelectron spectroscopy (XPS) analysis reveal that graphene oxide was reduced in hydrothermal process and BFO/RGO composites were successfully synthesized. UV-visible absorption and photoluminescence properties show that the introduction of RGO can effectively reduce the recombination of photogenerated electron and hole pairs. Compared to the pristine BFO, the photocatalytic performance of BiFeO3 Graphene Oxide (BGO) composites is enhanced for the degradation of Methylene blue (MB) solution under visible light irradiation, and the result shows that the optimal amount of Graphene Oxide (GO) in the composites is 60 mg (BGO60). The excellent photocatalytic performance is mainly ascribed to improved light absorption, increased reactive sites, and the low recombination rate of electron-hole pairs. This work can provide more insights into designing advanced photocatalysts for wastewater treatment and environmental protection.
Spontaneous Shape Alteration and Size Separation of Surfactant-Free Silver Particles Synthesized by Laser Ablation in Acetone during Long-Period Storage
Authors: Zhang Dongshi , Choi Wonsuk , Jakobi Jurij , Kalus Mark-Robert , Barcikowski Stephan , Cho Sung-Hak , Sugioka Koji
Published: July 2018

The technique of laser ablation in liquids (LAL) has already demonstrated its flexibility and capability for the synthesis of a large variety of surfactant-free nanomaterials with a high purity. However, high purity can cause trouble for nanomaterial synthesis, because active high-purity particles can spontaneously grow into different nanocrystals, which makes it difficult to accurately tailor the size and shape of the synthesized nanomaterials. Therefore, a series of questions arise with regards to whether particle growth occurs during colloid storage, how large the particle size increases to, and into which shape the particles evolve. To obtain answers to these questions, here, Ag particles that are synthesized by femtosecond (fs) laser ablation of Ag in acetone are used as precursors to witness the spontaneous growth behavior of the LAL-generated surfactant-free Ag dots (2–10 nm) into different polygonal particles (5–50 nm), and the spontaneous size separation phenomenon by the carbon-encapsulation induced precipitation of large particles, after six months of colloid storage. The colloids obtained by LAL at a higher power (600 mW) possess a greater ability and higher efficiency to yield colloids with sizes of <40 nm than the colloids obtained at lower power (300 mW), because of the generation of a larger amount of carbon ‘captors’ by the decomposition of acetone and the stronger particle fragmentation. Both the size increase and the shape alteration lead to a redshift of the surface plasmon resonance (SPR) band of the Ag colloid from 404 nm to 414 nm, after storage. The Fourier transform infrared spectroscopy (FTIR) analysis shows that the Ag particles are conjugated with COO– and OH– groups, both of which may lead to the growth of polygonal particles. The CO and CO2 molecules are adsorbed on the particle surfaces to form Ag(CO)x and Ag(CO2)x complexes. Complementary nanosecond LAL experiments confirmed that the particle growth was inherent to LAL in acetone, and independent of pulse duration, although some differences in the final particle sizes were observed. The nanosecond-LAL yields monomodal colloids, whereas the size-separated, initially bimodal colloids from the fs-LAL provide a higher fraction of very small particles that are <5 nm. The spontaneous growth of the LAL-generated metallic particles presented in this work should arouse the special attention of academia, especially regarding the detailed discussion on how long the colloids can be preserved for particle characterization and applications, without causing a mismatch between the colloid properties and their performance. The spontaneous size separation phenomenon may help researchers to realize a more reproducible synthesis for small metallic colloids, without concern for the generation of large particles.
Effect of Tb-doped Concentration Variation on the Electrical and Dielectric Properties of CaF2 Nanoparticles
Authors: Cui Xiaoyan , Hu Tingjing , Wang Jingshu , Zhong Xin , Chen Yinzhu , Zhang Junkai , Li Xuefei , Yang Jinghai , Gao Chunxiao
Published: July 2018

Calcium fluoride (CaF2) nanoparticles with various terbium (Tb) doping concentrations were investigated by X-ray diffraction (XRD), transmission electron microscopy (TEM), and alternating current (AC) impedance measurement. The original shape and structure of CaF2 nanoparticles were retained after doping. In all the samples, the dominant charge carriers were electrons, and the F− ion transference number increased with increasing Tb concentration. The defects in the grain region considerably contributed to the electron transportation process. When the Tb concentration was less than 3%, the effect of the ionic radius variation dominated and led to the diffusion of the F− ions and facilitated electron transportation. When the Tb concentration was greater than 3%, the increasing deformation potential scattering dominated, impeding F− ion diffusion and electron transportation. The substitution of Ca2+ by Tb3+ enables the electron and ion hopping in CaF2 nanocrystals, resulting in increased permittivity.
Extended Near-Infrared Photoactivity of Bi6Fe1.9Co0.1Ti3O18 by Upconversion Nanoparticles
Authors: Ge Wen , Li Zhiang , Chen Tong , Liu Min , Lu Yalin
Published: July 2018

Bi6Fe1.9Co0.1Ti3O18 (BFCTO)/NaGdF4:Yb3+, Er3+ (NGF) nanohybrids were successively synthesized by the hydrothermal process followed by anassembly method, and BFCTO-1.0/NGF nanosheets, BFCTO-1.5/NGF nanoplates and BFCTO-2.0/NGF truncated tetragonal bipyramids were obtained when 1.0, 1.5 and 2.0 M NaOH were adopted, respectively. Under the irradiation of 980 nm light, all the BFCTO samples exhibited no activity in degrading Rhodamine B (RhB). In contrast, with the loading of NGF upconversion nanoparticles, all the BFCTO/NGF samples exhibited extended near-infrared photoactivity, with BFCTO-1.5/NGF showing the best photocatalytic activity, which could be attributed to the effect of {001} and {117} crystal facets with the optimal ratio. In addition, the ferromagnetic properties of the BFCTO/NGF samples indicated their potential as novel, recyclable and efficient near-infrared (NIR) light-driven photocatalysts.
Estimation of Salt Intake Assessed by 24-Hour Urinary Sodium Excretion among Somali Adults in Oslo, Norway
Authors: Chen Sairah L. , Dahl Cecilie , Meyer Haakon E. , Madar Ahmed A.
Published: July 2018

High dietary salt intake is associated with increased blood pressure (BP) and cardiovascular disease (CVD) risk. The migration of Somalis from East Africa to Norway may have altered their dietary habits, making them vulnerable to adverse health outcomes. Since little is known about the lifestyle and health status of this population, the purpose of our study was to estimate salt intake in Somali adults in Oslo, Norway. In this cross-sectional study, we included 161 Somali adults (76 men, 86 women) from the Sagene borough in Oslo, Norway. Sodium and potassium excretion was assessed through the collection of 24-hour urine. Creatinine-based exclusions were made to ensure completeness of urine collections. Sodium excretion corresponding to an estimated dietary salt intake of 8.66 ± 3.33 g/24 h was found in men and 7.39 ± 3.64 g/24 h in women (p = 0.013). An estimated 72% of participants consumed >5 g salt/day. The Na:K ratio was 2.5 ± 1.2 in men and 2.4 ± 1.1 in women (p = 0.665). In conclusion, estimated salt intake was, while above the WHO recommendation, within the lower range of estimated salt intakes globally and in Western Europe. Further research is required to assess the health benefits of sodium reduction in this Somali immigrant population.
Anti-Osteoporotic Effects of Polysaccharides Isolated from Persimmon Leaves via Osteoclastogenesis Inhibition
Authors: Hwang Youn-Hwan , Ha Hyunil , Kim Rajeong , Cho Chang-Won , Song Young-Ran , Hong Hee-Do , Kim Taesoo
Published: July 2018

Persimmon (Diospyros kaki L.f.) leaves have traditionally been used as a phytomedicine, in health beverages to treat cardiovascular and respiratory disease and to promote maternal health in East Asia. In particular, polysaccharides from persimmon are known to have anti-coagulant, anti-oxidant, and immune-stimulatory activities. However, their beneficial effects against osteoporosis have not been reported. In the present study, we investigated the anti-osteoporotic effects of polysaccharides from persimmon leaves (PLE0) using an in vivo model of ovariectomy (OVX)-induced bone loss and an in vitro system of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. In the OVX mouse model, PLE0 remarkably improved OVX-induced trabecular bone loss by suppressing osteoclast activity. In primary bone marrow-derived macrophages (BMMs), PLE0 dose-dependently inhibited osteoclast differentiation. In addition, PLE0 down-regulated RANKL-induced activation of mitogen-activated protein kinases (MAPKs) such as p38, ERK, and JNK resulting in suppression of nuclear factor of activated T cells cytoplasmic 1 (NFATc1) expression. Our results indicate that PLE0 has anti-osteoporotic effects in OVX-induced bone loss via inhibition of osteoclast differentiation. Taken together, PLE0 from persimmon may prevent postmenopausal bone loss and osteoporotic bone fragility.
Immunomodulatory Protein Hydrolysates and Their Application
Authors: Kiewiet Mensiena B. G. , Faas Marijke M. , De Vos Paul
Published: July 2018

Immunomodulatory protein hydrolysate consumption may delay or prevent western immune-related diseases. In order to purposively develop protein hydrolysates with an optimal and reproducible immunomodulatory effect, knowledge is needed on which components in protein hydrolysates are responsible for the immune effects. Important advances have been made on this aspect. Also, knowledge on mechanisms underlying the immune modulating effects is indispensable. In this review, we discuss the most promising application possibilities for immunomodulatory protein hydrolysates. In order to do so, an overview is provided on reported in vivo immune effects of protein hydrolysates in both local intestinal and systemic organs, and the current insights in the underlying mechanisms of these effects. Furthermore, we discuss current knowledge and physicochemical approaches to identify the immune active protein sequence(s). We conclude that multiple hydrolysate compositions show specific immune effects. This knowledge can improve the efficacy of existing hydrolysate-containing products such as sports nutrition, clinical nutrition, and infant formula. We also provide arguments for why immunomodulatory protein hydrolysates could be applied to manage the immune response in the increasing number of individuals with a higher risk of immune dysfunction due to, for example, increasing age or stress.
In Silico SAR Studies of HIV-1 Inhibitors
Authors: Hdoufane Ismail , Bjij Imane , Soliman Mahmoud , Tadjer Alia , Villemin Didier , Bogdanov Jane , Cherqaoui Driss
Published: July 2018

Quantitative Structure Activity Relationships (QSAR or SAR) have helped scientists to establish mathematical relationships between molecular structures and their biological activities. In the present article, SAR studies have been carried out on 89 tetrahydroimidazo[4,5,1-jk][1,4]benzodiazepine (TIBO) derivatives using different classifiers, such as support vector machines, artificial neural networks, random forests, and decision trees. The goal is to propose classification models that will be able to classify TIBO compounds into two groups: high and low inhibitors of HIV-1 reverse transcriptase. Each molecular structure was encoded by 10 descriptors. To check the validity of the established models, all of them were subjected to various validation tests: internal validation, Y-randomization, and external validation. The established classification models have been successful. The correct classification rates reached 100% and 90% in the learning and test sets, respectively. Finally, molecular docking analysis was carried out to understand the interactions between reverse transcriptase enzyme and the TIBO compounds studied. Hydrophobic and hydrogen bond interactions led to the identification of active binding sites. The established models could help scientists to predict the inhibition activity of untested compounds or of novel molecules prior to their synthesis. Therefore, they could reduce the trial and error process in the design of human immunodeficiency virus (HIV) inhibitors.
Design and Synthesis of 99mTcN-Labeled Dextran-Mannose Derivatives for Sentinel Lymph Node Detection
Authors: Boschi Alessandra , Pasquali Micòl , Trapella Claudio , Massi Alessandro , Martini Petra , Duatti Adriano , Guerrini Remo , Zanirato Vinicio , Fantinati Anna , Marzola Erika , Giganti Melchiore , Uccelli Licia
Published: July 2018

Background: New approaches based on the receptor-targeted molecular interaction have been recently developed with the aim to investigate specific probes for sentinel lymph nodes. In particular, the mannose receptors expressed by lymph node macrophages became an attractive target and different multifunctional mannose derivate ligands for the labeling with 99mTc have been developed. In this study, we report the synthesis of a specific class of dextran-based, macromolecular, multifunctional ligands specially designed for labeling with the highly stable [99mTc≡N]2+ core. Methods: The ligands have been obtained by appending to a macromolecular dextran scaffold pendant arms bearing a chelating moiety for the metallic group and a mannosyl residue for allowing the interaction of the resulting macromolecular 99mTc conjugate with specific receptors on the external membrane of macrophages. Two different chelating systems have been selected, S-methyl dithiocarbazate [H2N‒NH‒C(=S)SCH3=HDTCZ] and a sequence of two cysteine residues, that in combination with a monophosphine coligand, are able to bind the [99mTc≡N]2+ core. Conclusions: High-specific-activity labeling has been obtained by simple mixing and heating of the [99mTc≡N]2+ group with the new mannose-dextran derivatives.
Determining Vortex-Beam Superpositions by Shear Interferometry
Authors: Khajavi Behzad , Ureta Junior , Galvez Enrique
Published: July 2018

Optical modes bearing optical vortices are important light systems in which to encode information. Optical vortices are robust features of optical beams that do not dissipate upon propagation. Thus, decoding the modal content of a beam is a vital component of the process. In this work, we present a method to decode modal superpositions of light beams that contain optical vortices. We do so using shear interferometry, which presents a simple and effective means of determining the vortex content of a beam, and extract the parameters of the component vortex modes that constitute them. We find that optical modes in a beam are easily determined. Its modal content can be extracted when they are of comparable magnitude. The use of modes of well-defined topological charge, but not well-defined radial-mode content, such as those produced by phase-only encoding, are much easier to diagnose than pure Laguerre–Gauss modes.
Bioactive and Bioadhesive Catechol Conjugated Polymers for Tissue Regeneration
Authors: Puertas-Bartolomé María , Vázquez-Lasa Blanca , San Román Julio
Published: July 2018

The effective treatment of chronic wounds constitutes one of the most common worldwide healthcare problem due to the presence of high levels of proteases, free radicals and exudates in the wound, which constantly activate the inflammatory system, avoiding tissue regeneration. In this study, we describe a multifunctional bioactive and resorbable membrane with in-built antioxidant agent catechol for the continuous quenching of free radicals as well as to control inflammatory response, helping to promote the wound-healing process. This natural polyphenol (catechol) is the key molecule responsible for the mechanism of adhesion of mussels providing also the functionalized polymer with bioadhesion in the moist environment of the human body. To reach that goal, synthesized statistical copolymers of N-vinylcaprolactam (V) and 2-hydroxyethyl methacrylate (H) have been conjugated with catechol bearing hydrocaffeic acid (HCA) molecules with high yields. The system has demonstrated good biocompatibility, a sustained antioxidant response, an anti-inflammatory effect, an ultraviolet (UV) screen, and bioadhesion to porcine skin, all of these been key features in the wound-healing process. Therefore, these novel mussel-inspired materials have an enormous potential for application and can act very positively, favoring and promoting the healing effect in chronic wounds.
Influence of Macromolecular Architecture on the Optical and Humidity-Sensing Properties of Poly(N,N-Dimethylacrylamide)-Based Block Copolymers
Authors: Lazarova Katerina , Vasileva Marina , Ivanova Sijka , Novakov Christo , Christova Darinka , Babeva Tsvetanka
Published: July 2018

The influence of the macromolecular architecture of block copolymers containing poly(N,N-dimethyl acrylamide) (PDMA) on the optical characteristics and sensing properties of corresponding thin films is discussed. Series of hydrophilic PDMA-based copolymers of different chemical composition and chain architecture such as triblock, star-shaped, and branched were synthesized. The copolymers were characterized using conventional spectroscopic techniques as well as methods for characterization of copolymer macromolecular characteristics in solution, namely size-exclusion chromatography and static light scattering. Thin films of the copolymers of nanometer scale thickness were deposited on silicon substrates by the spin-coating method. The refractive index and extinction coefficient of the copolymer films were calculated from the reflectance spectra by using non-linear curve fitting methods and the composition-structure-optical properties relationships were evaluated. Humidity-sensing properties of the films were studied by measuring reflectance spectra of the films at a relative humidity range from 5 to 95%RH. The implementation of the copolymer films as optical sensors of humidity is justified and discussed.
Development of Poly(HEMA-Am) Polymer Hydrogel Filler for Soft Tissue Reconstruction by Facile Polymerization
Authors: Kim Sujin , Shin Byung Ho , Yang Chungmo , Jeong Soohyun , Shim Jung Hee , Park Min Hee , Choy Young Bin , Heo Chan Yeong , Lee Kangwon
Published: July 2018

The number of breast reconstruction surgeries has been increasing due to the increase in mastectomies. Surgical implants (the standard polydimethylsiloxane (PDMS) implants) are widely used to reconstruct breast tissues, however, it can cause problems such as adverse immune reactions, fibrosis, rupture, and additional surgery. Hence, polymeric fillers have recently garnered increasing attention as strong alternatives for breast reconstruction materials. Polymeric fillers offer noninvasive methods of reconstruction, thereby reducing the possible adverse effects and simplifying the treatment. In this study, we synthesized a 2-hydroxylethylmethacrylate (HEMA) and acrylamide (Am) copolymer (Poly(HEMA-Am)) by redox polymerization to be used as a biocompatible filler material for breast reconstruction. The synthesized hydrogel swelled in phosphate buffered saline (PBS) shows an average modulus of 50 Pa, which is a characteristic similar to that of the standard dermal acrylamide filler. To investigate the biocompatibility and cytotoxicity of the Poly(HEMA-Am) hydrogel, we evaluated an in vitro cytotoxicity assay on human fibroblasts (hFBs) and human adipose-derived stem cells (hADSCs) with the hydrogel eluate, and confirmed a cell viability of over 80% of the cell viability with the Poly(HEMA-Am) hydrogel. These results suggest our polymeric hydrogel is a promising filler material in soft tissue augmentation including breast reconstruction.
Nanostructured Composites Based on Liquid-Crystalline Elastomers
Authors: Cresta Vanessa , Romano Giuseppe , Kolpak Alexej , Zalar Boštjan , Domenici Valentina
Published: July 2018

Liquid-crystalline elastomers (LCEs) are the object of many research investigations due to their reversible and controllable shape deformations, and their high potential for use in the field of soft robots and artificial muscles. This review focuses on recent studies about polymer composites based on LCEs and nanomaterials having different chemistry and morphology, with the aim of instilling new physical properties into LCEs. The synthesis, physico-chemical characterization, actuation properties, and applications of LCE-based composites reported in the literature are reviewed. Several cases are discussed: (1) the addition of various carbon nanomaterials to LCEs, from carbon black to carbon nanotubes, to the recent attempts to include graphene layers to enhance the thermo-mechanic properties of LCEs; (2) the use of various types of nanoparticles, such as ferroelectric ceramics, gold nanoparticles, conductive molybdenum-oxide nanowires, and magnetic iron-oxide nanoparticles, to induce electro-actuation, magnetic-actuation, or photo-actuation into the LCE-based composites; (3) the deposition on LCE surfaces of thin layers of conductive materials (i.e., conductive polymers and gold nanolayers) to produce bending actuation by applying on/off voltage cycles or surface-wrinkling phenomena in view of tunable optical applications. Some future perspectives of this field of soft materials conclude the review.
A Roadmap towards Successful Nanocapsule Synthesis via Vesicle Templated RAFT-Based Emulsion Polymerization
Authors: Rusli Wendy , Jackson Alexander W. , Van Herk Alexander
Published: July 2018

Vesicle templated emulsion polymerization is a special form of emulsion polymerization where the polymer is grown from the outside of the vesicle, leading to nanocapsules. Cost effective nanocapsules synthesis is in high demand due to phasing out of older methods for capsule synthesis. Although the first indications of this route being successful were published some 10 years ago, until now a thorough understanding of the parameters controlling the morphologies resulting from the template emulsion polymerization was lacking. Most often a mixture of different morphologies was obtained, ranging from solid particles to pro-trusion structures to nanocapsules. A high yield of nanocapsules was not achieved until now. In this paper, the influence of initial vesicle dispersion, choice of the Reversible Addition-Fragmentation chain Transfer (RAFT) species and oligomer, monomer and crosslinker have been investigated. It turns out that good initial vesicle dispersion, molecular control of the RAFT process, a not too hydrophobic monomer and some crosslinking is needed to result in high yield of nanocapsules. In previous work, the level of RAFT control was often suboptimal and not properly verified and although nanocapsules were shown, other morphologies were also present. We now believe we have a full understanding of vesicle templated nanocapsules synthesis, relevant to many applications.
In Situ Strain and Damage Monitoring of GFRP Laminates Incorporating Carbon Nanofibers under Tension
Authors: Wang Yanlei , Wang Yongshuai , Han Baoguo , Wan Baolin , Cai Gaochuang , Chang Ruijuan
Published: July 2018

In this study, conductive carbon nanofibers (CNFs) were dispersed into epoxy resin and then infused into glass fiber fabric to fabricate CNF/glass fiber-reinforced polymer (GFRP) laminates. The electrical resistance and strain of CNF/GFRP laminates were measured simultaneously during tensile loadings to investigate the in situ strain and damage monitoring capability of CNF/GFRP laminates. The damage evolution and conduction mechanisms of the laminates were also presented. The results indicated that the percolation threshold of CNFs content for CNF/GFRP laminates was 0.86 wt % based on a typical power law. The resistance response during monotonic tensile loading could be classified into three stages corresponding to different damage mechanisms, which demonstrated a good ability of in situ damage monitoring of the CNF/GFRP laminates. In addition, the capacity of in situ strain monitoring of the laminates during small strain stages was also confirmed according to the synchronous and reversible resistance responses to strain under constant cyclic tensile loading. Moreover, the analysis of the resistance responses during incremental amplitude cyclic tensile loading with the maximum strain of 1.5% suggested that in situ strain and damage monitoring of the CNF/GFRP laminates were feasible and stable.
Identification and Characterization of Novel Fc-Binding Heptapeptides from Experiments and Simulations
Authors: Sun Xiaoquan , Weaver Justin , Wickramasinghe Sumith Ranil , Qian Xianghong
Published: July 2018

Purification of biologically-derived therapeutics is a major cost contributor to the production of this rapidly growing class of pharmaceuticals. Monoclonal antibodies comprise a large percentage of these products, therefore new antibody purification tools are needed. Small peptides, as opposed to traditional antibody affinity ligands such as Protein A, may have advantages in stability and production costs. Multiple heptapeptides that demonstrate Fc binding behavior that have been identified from a combinatorial peptide library using M13 phage display are presented herein. Seven unique peptide sequences of diverse hydrophobicity and charge were identified. All seven peptides showed strong binding to the four major human IgG isotypes, human IgM, as well as binding to canine, rat, and mouse IgG. These seven peptides were also shown to bind human IgG4 from DMEM cell culture media with 5% FCS and 5 g/L ovalbumin present. These peptides may be useful as surface ligands for antibody detection and purification purposes. Molecular docking and classical molecular dynamics (MD) simulations were conducted to elucidate the mechanisms and energetics for the binding of these peptides to the Fc region. The binding site was found to be located between the two glycan chains inside the Fc fragment. Both hydrogen bonding and hydrophobic interactions were found to be crucial for the binding interactions. Excellent agreement for the binding strength was obtained between experimental results and simulations.