bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Study of Phosphorous-Acid-Containing Products for Managing Phytophthora Blight of Bell Pepper
Authors: Hua Gia Khuong Hoang , Ji Pingsheng , Culbreath Albert K. , Ali Md Emran
Published: May 2022

Phytophthora blight of pepper caused by Phytophthora capsici is a major constraint to bell pepper (Capsicum annuum) production. The long-term effectiveness of chemicals currently in use against P. capsici is uncertain due to the development of fungicide resistance by this pathogen. Hence, the efficacy of alternative chemicals such as phosphorous-acid-containing products was evaluated in this study. In in vitro tests, ProPhyt, K-Phite, Lexx-A-Phos, Agri-Fos, and Nutri-Phite were less effective in inhibiting mycelial growth (EC50 = 50.5 to 324.4 µg mL−1) and sporangium formation (EC50 = 6.1 to 225.7 µg mL−1) of two P. capsici isolates, but more effective against zoospore germination compared with mefenoxam. Among phosphorous-acid-containing products tested, Nutri-Phite was most effective in inhibiting mycelial growth of both P. capsici isolates. In greenhouse studies, Nutri-Phite was effective against Phytophthora blight used as drench. The use of Nutri-Phite, Agri-Fos, ProPhyt, and K-Phite could induce systemic resistance against foliar blight when applied to the root and potting mix. The results indicated that some phosphorous-acid-containing products have the potential to lower disease occurrence and delay Phytophthora blight of bell pepper without phytotoxic effects. The utility of the systemic protection induced by these products is promising in Phytophthora blight management.
Development of the PARMS Markers of the Waxy Gene and Utilization in Discriminating Wild Accessions, and Cultivated Rice (Oryza sativa L.) with Different Eating and Cooking Quality
Authors: Jeazet Dongho Epse Mackon Guibeline Charlie , Mackon Enerand , Ma Yafei , Zhao Yitong , Yao Yuhang , Dai Xianggui , Liu Piqing
Published: May 2022

Amylose content (AC) is the major indicator of rice eating and cooking quality (ECQ). Its synthesis in rice endosperm is mainly regulated by the protein, granule-bound starch synthase 1, which is encoded by the waxy gene (Os06g0133000, LOC_Os06g04200). The diversity of AC is largely attributable to the allelic variation at the Wx locus and the development of effective and accurate functional molecular markers to target rice variant alleles is crucial in the breeding strategy. In the present study, we developed six pairs of penta-primer amplification refractory mutation system (PARMS) markers to distinguish between Wxlv, Wxa, Wxin, Wxb, Wxmp, and Wxop,hp. These markers were successfully used to screen the genotype of large assets of genetic resources including 98 wild accessions, 55 cultivars, and 22 parental lines. Our results showed that Wxb in a low AC type was predominant in Guangxi cultivated rice as a result of cultural preference, while Wxlv in the wild accessions. Moreover, our findings surprisingly revealed the presence of Wxb in wild accession, which is a new outcome that may contribute to understanding the origin, selection and domestication processes of rice. These functional markers could be effectively used in marker-assisted breeding to improve selection efficiency of cultivars with desired AC in the early generation.
Simultaneous Estimation of Rhein and Aloe-Emodin in Traditional and Ultrasound-Based Extracts of Rheum palmatum L. (Rhubarb) Using Sustainable Reverse-Phase and Conventional Normal-Phase HPTLC Methods
Authors: Alqarni Mohammed H. , Alam Prawez , Shakeel Faiyaz , Alam Aftab , Salkini Mohammad A. , Muharram Magdy M.
Published: May 2022

The greenness indices of literature analytical procedures for the simultaneous measurement of rhein and aloe-emodin have not been determined. As a consequence, the first goal of this study was to design and validate a sensitive and sustainable reverse-phase high-performance thin-layer chromatography (HPTLC) method for the simultaneous estimation of rhein and aloe-emodin in a traditional extract (TE) and ultrasound-based extract (UBE) of commercial Rhubarb and Rhubarb plant extracts in comparison to the conventional normal-phase HPTLC method. The second goal was to determine the greenness indices for both methods using the AGREE approach. For the sustainable reverse-phase HPTLC approach, the method was linear in the 50–1000 ng/spot range for rhein and 25–1000 ng/spot range for aloe-emodin. However, for the conventional normal-phase HPTLC approach, the method was linear in the 50–600 ng/spot range for rhein and 100–600 ng/spot range for aloe-emodin. The limit of detection (LOD) for rhein and aloe-emodin was 16.81 ng/spot and 8.49 ng/spot, respectively, using the sustainable analytical method. However, the LOD for rhein and aloe-emodin was 18.53 ng/spot and 39.42 ng/spot, respectively, using the conventional analytical method. For the simultaneous determination of rhein and aloe-emodin, the sustainable analytical method was more sensitive, accurate, precise, and robust than the conventional analytical method. The amount of rhein and aloe-emodin was higher in the UBE of commercial Rhubarb and Rhubarb plant extract over their TE. For the simultaneous quantification of rhein and aloe-emodin in the TE and UBE of marketed Rhubarb and Rhubarb plant extract, the sustainable analytical method was superior to the conventional analytical method. The AGREE index for the sustainable reverse-phase and conventional normal-phase HPTLC methods was determined to be 0.78 and 0.49, respectively, indicating an excellent greenness profile of the sustainable reverse-phase HPTLC method over the conventional normal-phase HPTLC approach. The sustainable analytical method was found to be superior to the conventional analytical method based on these results.
Combining Planting Patterns with Mulching Bolsters the Soil Water Content, Growth, Yield, and Water Use Efficiency of Spring Wheat under Limited Water Supply in Arid Regions
Authors: El-Hendawy Salah , Alsamin Bazel , Mohammed Nabil , Al-Suhaibani Nasser , Refay Yahya , Alotaibi Majed , Tola ElKamil , Mattar Mohamed A.
Published: May 2022

Innovations in water-saving cultivation strategies are urgently needed to achieve high yield and elevated water use efficiency (WUE) simultaneously in arid regions with limited water resources. Here, we conducted a two-year field study to compare the impacts of eight combinations of planting patterns (PPs) and mulching on the soil water content (SWC) in the top 60 cm soil layer, the growth, the yield, and the WUE of wheat under two irrigation rates (1.00 and 0.50 ET). These combinations included three conventional flat planting (CF) patterns, including CF without mulch (CFNM), with plastic film (CFPM), and with wheat straw mulch (CFSM); three raised-bed planting (RB) patterns, including RB without mulch (RBNM), with plastic film (RBPM), and wheat straw (RBSM) mulch; and two ridge–furrow planting (RF) patterns, including RF without mulch (RFNM) and with plastic film mulch (RFPM). The results showed that the tested treatments affected the SWC at different depths under both irrigation rates. Compared with the two non-mulched treatments under 0.50 ET, the SWC of the three PPs with plastic film and the two PPs with wheat straw mulching were significantly higher before irrigation by 14.4–22.0% and 6.9–17.2% at 0–20 cm soil depth, 16.4–29.0% and 6.6–14.9% at 20–40 cm soil depth, and 3.3–34.8% and 3.4–14.5% at 40–60 cm soil depth, respectively. All measured wheat parameters, except harvest index, were significantly affected by the interaction between irrigation rate and PPs. The highest values for plant dry weight (PDW), yield components, grain yield (GY), and WUE under 1.00 ET were obtained in the two PPs with wheat straw mulch, while the three PPs with plastic film showed the highest values of these parameters under 0.50 ET. The yield response factor (Ky) based on PDW was acceptable for all PPs mulched with plastic film and wheat straw as well as for RFNM, while Ky based on GY was acceptable only for the PPs mulched with plastic film and for RFNM, as the Ky values of these PPs were less than 1 under 0.50 ET. The SWC at different depths exhibited quadratic and nonsignificant relationships with all parameters under 1.00 ET, while these relationships were linear and strong under 0.50 ET, with a few exceptions. Overall, we conclude that combining any PPs with plastic film mulching could be used as a feasible and effective strategy for obtaining high wheat yield and WUE in the irrigated and arid agroecosystem.
An Agricultural Event Prediction Framework towards Anticipatory Scheduling of Robot Fleets: General Concepts and Case Studies
Authors: Pal Abhishesh , Das Gautham , Hanheide Marc , Candea Leite Antonio , From Pål Johan
Published: May 2022

Harvesting in soft-fruit farms is labor intensive, time consuming and is severely affected by scarcity of skilled labors. Among several activities during soft-fruit harvesting, human pickers take 20–30% of overall operation time into the logistics activities. Such an unproductive time, for example, can be reduced by optimally deploying a fleet of agricultural robots and schedule them by anticipating the human activity behaviour (state) during harvesting. In this paper, we propose a framework for spatio-temporal prediction of human pickers’ activities while they are picking fruits in agriculture fields. Here we exploit temporal patterns of picking operation and 2D discrete points, called topological nodes, as spatial constraints imposed by the agricultural environment. Both information are used in the prediction framework in combination with a variant of the Hidden Markov Model (HMM) algorithm to create two modules. The proposed methodology is validated with two test cases. In Test Case 1, the first module selects an optimal temporal model called as picking_state_progression model that uses temporal features of a picker state (event) to statistically evaluate an adequate number of intra-states also called sub-states. In Test Case 2, the second module uses the outcome from the optimal temporal model in the subsequent spatial model called node_transition model and performs “spatio-temporal predictions” of the picker’s movement while the picker is in a particular state. The Discrete Event Simulation (DES) framework, a proven agricultural multi-robot logistics model, is used to simulate the different picking operation scenarios with and without our proposed prediction framework and the results are then statistically compared to each other. Our prediction framework can reduce the so-called unproductive logistics time in a fully manual harvesting process by about 80 percent in the overall picking operation. This research also indicates that the different rates of picking operations involve different numbers of sub-states, and these sub-states are associated with different trends considered in spatio-temporal predictions.
Mitigation of Flood Risks with the Aid of the Critical Points Method
Authors: Drbal Karel , Dumbrovský Miroslav , Muchová Zlatica , Sobotková Veronika , Štěpánková Pavla , Šarapatka Bořivoj
Published: May 2022

Concentrated surface run-off caused by torrential rain leads to the transport of sediments from soil erosion processes within catchment areas of critical points, which represents a basic component of flood risks. Clear identification of such critical points offers a basis for a suitable new strategy of threat mitigation, via both organizational and structural measures in catchment areas. Critical points are determined in places where generated paths of concentrated surface run-off cross given boundaries of built-up areas. The threshold values for the catchment area of a critical point were derived on the basis of hydrological calculations, field research, and the evaluation of hundreds of specific manifestations of damage in built-up areas for particular critical profiles. The characteristics were determined as follows: contributing area between 0.3 and 10 km2, average slope more than 3.5%, and percentage of arable land more than 40%. Indicator F was determined for the distribution of the territory of the Czech Republic according to the risk of flooding. Knowledge of the existence of critical points enables the implementation of preventive measures, the evaluation of flood risk associated with the transport of sediment due to erosion processes, and the improvement of management measures in respective catchment areas, even before an event occurs. The proposed procedure outputs shall be reflected in spatial planning documentation, land consolidation, and catchment area management plans. Incorporation of critical points into open-access public web-maps can help with assessing the semi-quantitative expression of risk to built-up areas arising from the threat of local flooding.
Priestia sp. LWS1 Is a Selenium-Resistant Plant Growth-Promoting Bacterium That Can Enhance Plant Growth and Selenium Accumulation in Oryza sativa L.
Authors: Lin Xiao-Rui , Chen Han-Bing , Li Yi-Xi , Zhou Zhi-Hua , Li Jia-Bing , Wang Yao-Qiang , Zhang Hong , Zhang Yong , Han Yong-He , Wang Shan-Shan
Published: May 2022

Selenium (Se) is essential for the basic functions of life, but the low daily intake of Se urges us to find reliable ways to increase food Se content. Plant-growth-promoting bacteria (PGPB) have shown potential in enhancing plant growth and Se accumulation. In this study, the soils collected from a Se tailing were used to isolate Se-tolerant PGPB. The results showed that a total of three strains were identified. Strain LWS1, belonging to Priestia sp., grew well in M9 medium and exhibited typical PGP characteristics by an IAA-production ability of 24.3 ± 1.37 mg·L−1, siderophore-production ability of 0.23 ± 0.04 and phosphate-solubilizing ability of 87.5 ± 0.21 mg·L−1. Moreover, LWS1 strain tolerated selenite (SeIV) up to 90 mM by a LC50 of 270.4 mg·L–1. Further investigations demonstrated that the inoculation of strain LWS1 resulted in up to 19% higher biomass and 75% higher Se concentration in rice (Oryza sativa L.) than uninoculated treatments. Our study has provided evidence that microbial Se biofortification through inoculating with Priestia sp. strain LWS1 is an alternative way to improve Se uptake in crops and maintain human health.
Comparison of Water Sensitive Paper and Glass Strip Sampling Approaches to Access Spray Deposit by UAV Sprayers
Authors: Ahmad Fiaz , Zhang Songchao , Qiu Baijing , Ma Jing , Xin Huang , Qiu Wei , Ahmed Shibbir , Chandio Farman Ali , Khaliq Aftab
Published: May 2022

Target and off-target spray depositions determine the spray’s effectiveness and impact on the environment. A decisive stage in the measurement of spray deposition and drift is selecting an appropriate sampling approach under field conditions. There are various approaches available for sampling spray deposition and drift, during the evaluation of ground sprayers used for the UAV sprayer assessment, under field conditions. In this study, two sampling approaches (water-sensitive paper, and glass strip collectors) were compared to analyze spray deposition in target and off-target zones. The results showed a variation in the estimation of the spray deposits among the two applied sampling methods. The results showed that the water-sensitive paper recorded the droplet deposition in the target zone with a range from 0.049 to 4.866 µLcm−2, whereas the glass strip recorded from 0.11 to 0.793 µLcm−2. The results also showed the water sensitive paper recorded an 80.3% higher deposition than that of the glass strip at zero position during the driving flight height 2 m and flight speed 2 ms−1 (T1 treatment). It can be concluded that variation in recorded depositing is due to the sampling material. It is recommended that the confident deposition results, measurement methods and sampling approaches must be standardized for UAV sprayers according to the field conditions and controlled within artificial assessments.
Production of Nutrient-Enriched Vermicompost from Aquatic Macrophytes Supplemented with Kitchen Waste: Assessment of Nutrient Changes, Phytotoxicity, and Earthworm Biodynamics
Authors: Yatoo Ali Mohd , Bhat Sartaj Ahmad , Ali Md. Niamat , Baba Zahoor Ahmad , Zaheen Zarka
Published: May 2022

Vermicompost is an organic fertilizer rich in nutrients, beneficial microbes, and plant growth hormones that not only enhances the growth of crops but also contributes to the improvement in the physicochemical and biological properties of the soil. However, its lower nutrient content makes it less preferable among farmers and limits its applicability. Here, we investigate, for the first time, nutrient enrichment of vermicompost by supplementing the free-floating macrophyte biomass with cow manure and organic nutrient supplements (eggshell, bone meal, banana peel, and tea waste). Free-floating macrophytes are aquatic plants that are found suspended on the water surface, playing a significant role in the structural and functional aspects of aquatic ecosystems. However, uncontrolled proliferation of these macrophytes endangers these ecosystems, having both economic and ecological implications; therefore, they need to be managed. Results showed an enhanced total nitrogen (2.87%), total phosphorus (0.86%), total potassium (3.74%), and other nutrients in vermicompost amended with cow manure and nutrient supplements. Highest biomass gain (710–782 mg), growth rate (11.83–13.04 mg), and reproduction rate (3.34–3.75 cocoons per worm) was also observed, indicating that amending bulking agent and nutrient supplements not only enhance the nutrient content of the final product but also improve overall earthworm activity. The stability and maturity of vermicompost, as indicated by C/N (<20) and Germination Index (>80), indicates that vermicompost obtained is suitable for agricultural applications. The study concluded that amendment of cow manure and organic nutrient supplements results in producing mature and nutrient-enriched vermicompost suitable for sustainable agricultural production.
Nitrogen Hotspots on the Farm—A Practice-Oriented Approach
Authors: Grzebisz Witold , Niewiadomska Alicja , Przygocka-Cyna Katarzyna
Published: May 2022

The proactive management of nitrogen (N) on a farm is the best way to protect the environment against N pollution. The farm is the basic business unit, where simple and low-cost methods of identifying and ameliorating weaknesses (nitrogen hotspots) in the N-flow chain can be applied. The basis for the effective use of mineral N fertilizers (Nf) is the farmer’s knowledge of the farm’s own N resources, their quantity, and the potential availability for growing crops. These resources include both primary sources of N (N2 fixed by legumes) and those that are recyclable, which include crop byproducts and manure. On the other hand, crop requirements must be accurately quantified to exploit the yield potential of the crop varieties grown on the farm. The basic challenge for the farmer is to maximize the use efficiency of the N resources. In this regard, the farmer has two diagnostic tools available to recognize nitrogen hotspots and to quantify N resources. These are (1) the N balance method (difference between the N inputs and outputs), which allows for a surplus or deficiencies in the N-flow between farm units (fields, livestock housing) to be identified, and (2) the nitrogen gap, which is based on the amount of Nf applied and the yield of a given crop. It is possible to calculate the maximum attainable yield as well as identify the fields on the farm that require a correction of N management.
Soil Water Dynamics and Cotton Production Following Cover Crop Use in a Semi-Arid Ecoregion
Authors: Burke Joseph Alan , Lewis Katie Lynn , DeLaune Paul Bradley , Cobos Christopher Joseph , Keeling Jack Wayne
Published: May 2022

Conservation management practices such as no-tillage and cover crops can decrease soil’s susceptibility to wind erosion, but adoption of these practices has been limited on the Texas High Plains (THP) where producers are concerned with cover crop water usage. The objective of this study was to evaluate the impact of no-tillage and cover crops on cotton (Gossypium hirsutum L.) lint yield and soil water content in a deficit irrigated cropping system. Soil water was observed bi-weekly in long-term, continuous cotton systems established in 1998 that included (1) conventional tillage, winter fallow, (2) no-tillage with rye (Secale cereale L.) cover, and (3) no-tillage with mixed species cover located in Lamesa, TX, USA. Results include observations from 2018–2020 (years 21–23 of the study period). The adoption of conservation practices did not significantly reduce cotton lint yield compared to conventionally tilled, winter fallow cotton. Soil water was initially depleted with cover crops but was greater throughout the growing season following cover crop termination. Throughout the soil profile, water depletion and recharge were more dynamic with conservation practices compared to the conventionally tilled control. There were no differences in cotton water use efficiency between treatments. Results from this study indicate cover crop water usage is likely not the cause of cotton lint yield decline in this deficit irrigated semi-arid production system.
A Comparison between Conventional Sprayers and New UAV Sprayers: A Study Case of Vineyards and Olives in Extremadura (Spain)
Authors: Morales-Rodríguez Pablo Antonio , Cano Cano Eugenio , Villena Jaime , López-Perales Jesús Antonio
Published: May 2022

Recently, technological development has become increasingly pronounced, with great advances in all production areas, including agriculture. In the agricultural sector, improvements in machinery and the study of new techniques have resulted in the increased efficiency and productivity of different crops. In particular, UAVs (unmanned aerial vehicles) are increasingly used in agriculture. Among the latest utilities is the application of phytosanitary products. The use of small UAVs retrofitted with spraying systems make it possible to treat large surfaces in short periods of time, achieving a reduction in inputs and time compared to conventionally used methods (trailed sprayers). The aim of this work was to compare conventional sprayers and UAV sprayers on a farm in Spain, with olive and vineyard crops. The determining factors in the choice of one or another alternative were studied, such as the economic requirements and efficiency of different alternatives. The results show that a large investment is required to acquire a UAV sprayer, while operating costs are higher for conventional sprayers versus UAV sprayers. Efficiency (ha/h) is much higher for UAV sprayers. In addition to these advantages, UAV sprayers require a lower cost of water and products. However, different factors could make conventional sprayers a better choice.
Nitrogen Application and Dense Planting to Obtain High Yields from Maize
Authors: Zhai Juan , Zhang Yuanmeng , Zhang Guoqiang , Xu Wenqian , Xie Ruizhi , Ming Bo , Hou Peng , Wang Keru , Xue Jun , Li Shaokun
Published: May 2022

The rational application of nitrogen fertilizer and close planting are two important ways to obtain high yields and efficient growth from maize (Zea mays L.). This study utilized the maize varieties DengHai 618 and XianYu 335 as test materials from 2019 to 2020 and the maize variety XianYu 335 as the test material in 2021. The planting densities were 7.5 × 104 plants ha−1 and 12.0 × 104 plants ha−1, respectively. Application rates of nitrogen within the range of 0–765 kg ha−1 per 45 kg ha−1 were considered the nitrogen application gradient. The results showed that as the nitrogen application rate increased, the yield of the maize increased at first and then tended to remain flat. Under conditions of 7.5 × 104 plants ha−1 density, the best yield was 17.6–20.2 t ha−1, and the required nitrogen application rate was 219–337 kg ha−1. Under conditions of 12.0 × 104 plants ha−1 density, the best yield was 18.7–21.9 t ha−1, and the required nitrogen application rate was 243–378 kg ha−1. With the increase in the nitrogen application rate, the dry matter weight showed a linear/platform relationship in each growth period. The best nitrogen application rate was obtained for dry matter accumulation in various stages by fitting the nitrogen application rate and dry matter accumulation in different stages. It is concluded that when the planting density was 7.5 × 104 plants ha−1, the recommended nitrogen application rate was 340 kg ha−1, and the distribution ratio of the nitrogen application rates before and after silking were 61.2% and 38.8%, respectively. When the planting density was 12.0 × 104 plants ha−1, the recommended nitrogen application rate was 380 kg ha−1, and the distribution rates before and after flowering were 65.8% and 34.2%, respectively. In summary, increasing planting density can improve maize yield, and the amount of nitrogen applied should be increased before flowering.
The Knockout of the ASIP Gene Altered the Lipid Composition in Bovine Mammary Epithelial Cells via the Expression of Genes in the Lipid Metabolism Pathway
Authors: Xie Tao , Liu Yinuo , Lu Huixian , Iqbal Ambreen , Ruan Mengru , Jiang Ping , Yu Haibin , Meng Jilun , Zhao Zhihui
Published: May 2022

Agouti signalling protein (ASIP) is a coat colour-related protein and also is a protein-related to lipid metabolism, which had first been found in agoutis. According to our previous study, ASIP is a candidate gene that affects the lipid metabolism in bovine adipocytes. However, its effect on milk lipid has not been reported yet. This study focused on the effect of the ASIP gene on the lipid metabolism of mammary epithelial cells in cattle. The ASIP gene was knocked out in bMECs by using CRISPR/Cas9 technology. The result of transcriptome sequencing showed that the differentially expressed genes associated with lipid metabolism were mainly enriched in the fatty acids metabolism pathways. Furthermore, the contents of intracellular triglycerides were significantly increased (p < 0.05), and cholesterol tended to rise (p > 0.05) in bMECs with the knockout of the ASIP gene. Fatty acid assays showed a significant alteration in medium and long-chain fatty acid content. Saturated and polyunsaturated fatty acids were significantly up-regulated (p < 0.05), and monounsaturated fatty acids were significantly decreased in the ASIP knockout bMECs (p < 0.05). The Q-PCR analysis showed that knockout of ASIP resulted in a significant reduction of gene expressions like PPARγ, FASN, SCD, and a significant up-regulation of genes like FABP4, ELOVL6, ACSL1, HACD4 prompted increased mid-to long-chain fatty acid synthesis. Overall, ASIP plays a pivotal role in regulating lipid metabolism in bMECs, which could further influence the component of lipid in milk.
Toxoplasma gondii and Neospora caninum Infections in Stray Cats and Dogs in the Qinghai–Tibetan Plateau Area, China
Authors: Yang Jinfang , Ai Jingkai , Qi Tongsheng , Ni Xiaomin , Xu Zichun , Guo Liangting , Sun Yali , Li Ying , Kang Ming , Li Jixu
Published: May 2022

Toxoplasma gondii and Neospora caninum belong to the Apicomplexan protozoa which is an obligate intracellular parasite, causing toxoplasmosis and neosporosis throughout the world. Cats and dogs are the definitive hosts of these two parasites. However, information on the epidemiology of toxoplasmosis and neosporosis in stray cats and dogs in the Qinghai–Tibetan Plateau Area (QTPA) is limited, and little is known about the diversity of the diseases. Therefore, the aim of this study was to perform indirect ELISA tests based on recombinant TgSAG1, TgGRA1, NcSAG1 and NcGRA7 proteins to establish a detailed record of the seroprevalence of T. gondii and N. caninum-specific IgG and IgM antibodies in serum samples and to develop qPCR amplification based on TgB1 and NcNc5 genes to conduct molecular epidemiology in feces from stray cats and dogs in the QTPA. In the current study, a total of 128 cat serum samples were analyzed through serological tests in which 53 (41.4%) and 57 (44.5%) samples were found positive for T. gondii specific-IgG and IgM antibodies, and 2 (1.6%) and 74 (57.8%) samples were confirmed positive for N. caninum specific-IgG and IgM antibodies, respectively. Out of 224 stray dog sera, 59.8% and 58.9% were recorded as positive against anti-Toxoplasma IgG and IgM antibodies, 17.9% and 64.7% were detected positive against Neospora IgG and IgM. On the other hand, 1 of 18 cat fecal samples was successfully amplified within the Ct value of 10 to 30 while no cat was positive for neosporosis. Moreover, a higher prevalence of toxoplasmosis in stray dogs (14.5%, 16/110) than of neosporosis (5.5%, 6/110) with different parasite numbers were found. Further analysis showed that no significant sex differences were found nor between the overall infection rates of T. gondii and N. caninum in this study. This study suggests that stray cats and dogs play key roles in the transmission and prevalence of T. gondii and N. caninum in the plateau area.
Direct Rub Inoculation of Fungal Flora Changes Fatty Acid Composition and Volatile Flavors in Dry-Aged Beef: A Preliminary Study
Authors: Mikami Nana , Toyotome Takahito , Takaya Masahiro , Tamura Kenichi
Published: May 2022

Here, we established a method to produce dry-aged beef (DAB) by rub inoculation with fungal flora on the prepared DAB surface. Portions of Holstein steers’ rumps were prepared by direct rub inoculation of fungal flora or without treatment (conventional DAB) and dry-aged for 26 days in an aging room at 2.9 °C and 90% relative humidity. We compared the fungal covering and meat quality, including fatty acid composition and volatile aromatic compounds, of fungal-inoculated DAB with those of the conventional DAB. The fungal-inoculated DAB was almost entirely covered with white mold, in contrast to the conventional DAB. Moreover, the proportion of oleic acid and the concentration of nine volatile compounds significantly increased in the raw meat of fungal-inoculated DAB compared with those in the conventional DAB (p < 0.05). These results suggested that direct rub inoculation of fungal flora from prepared DAB may accelerate DAB production and efficiently enhance the “melt-in-the-mouth” feeling and flavors of DAB.
Northward Range Expansion of Water Deer in Northeast Asia: Direct Evidence and Management Implications
Authors: Li Ying , Kim Jee Hyun , Li Hailong , Peng Yuxi , Chen Min , Zhu Weihong , Pandey Puneet , Sedash Gleb , Wang Tianming , Darman Yury , Lee Hang
Published: May 2022

Given current anthropogenic pressures and climate change, wildlife range expansion offers a second chance for species conservation. The water deer Hydropotes inermis is a native to China and the Korean peninsula, but populations in North Korea and mainland China have declined drastically in recent years. However, the range of this species appears to be rapidly expanding northward. In this study, we employed camera traps and molecular technology to assess the status, phylogeny, and genetic ancestry of the newly recorded population. Our results showed an ongoing northward expansion of water deer, reaching at least 500 km from their historical distribution limit. We provided updated information on this species’ geographical distribution in Northeast China and the Russian Far East. Based on historical survey data before the 1990s, there had previously been no record of this species in these two regions; thus, this could be a genuine range expansion rather than simply an expansion of the known range. A genetic investigation based on mitochondrial DNA indicated that the expanding population had a close phylogenetic affinity with Korean water deer. The likely migration route and causes of the species’ distribution range expansion are discussed. We recommend revising the range of water deer in the IUCN Red List to facilitate the effective conservation and management of this threatened species, especially in new locations.
Comparison of the Current Situation of Equine Headshaking Syndrome in France and Switzerland Based on an Online Survey
Authors: Stange Laura Maxi , Krieter Joachim , Czycholl Irena
Published: May 2022

Equine headshaking syndrome (EHS) is characterised as non-physical and involuntary movement of the horse’s head and neck. Although EHS is clinically simple to diagnose, its aetiopathogenesis often remains unclear. The aim of this study was to gain an overview of signalment and therapy possibilities used in France and Switzerland. To do this, an online survey was developed and distributed via newsletters. A total of 933 complete, answered surveys from France (n = 804) and Switzerland (n = 129) were evaluated. The median age in France was 12.4 years (CH = 14.3). Mostly geldings were affected (58.5%FRA, 57.4%CH). There was an association with Warmbloods in Switzerland (55.8%CH), but in France, in addition to Warmbloods (34.4%FRA), Thoroughbreds (27.2%FRA) were also affected. Moreover, horses affected by EHS often show stereotypical behaviour (15.7%FRA, 14.7%CH). A total of 38.4%FRA and 67.4%CH of horse owners utilised therapy measures, with nose covers being most commonly used (19.9%FRA, 30.2%CH). Horse owners resorted to alternative treatments that had not previously been studied in context with EHS (15%FRA, 20.9%CH). Conservative treatments, such as medication, were used by 5.4%CH and 1.9%FRA. This study provides an overview of the status of horses affected by EHS in France and Switzerland and thus offers a fundamental step to understanding the consequences of welfare issues associated with EHS.
Subclinical Mastitis Detected during the Last Gestation Period Can Increase the Risk of Stillbirth in Dairy Calves
Authors: Antanaitis Ramūnas , Juozaitienė Vida , Jonike Vesta , Baumgartner Walter , Paulauskas Algimantas
Published: May 2022

We hypothesized that subclinical mastitis detected during the last gestation period can increase the risk of stillbirth in dairy calves. The aim was to investigate the relation of subclinical mastitis detected during the last gestation period and its pathogens with the stillbirth of calves. Cows from the 210th day of pregnancy were selected for the study. They were divided into two groups: the first group—subclinical mastitis was confirmed on the farm by the California mastitis test (CMT); the second group of cows—mastitis was not confirmed by the CMT test. Groups of cows were compared according to the results of their calving—the number of stillborn calves. A stillborn calf was defined as a calf that dies at birth or within the first 24 h after calving, following a gestation period of 260 days. Our results suggest that decreasing the incidence of subclinical mastitis during the last gestation period (from the 210th day of pregnancy) can decrease the risk of stillbirth in dairy calves. Further, it is important to identify the pathogen because the highest risk of stillbirth was found in cows with mastitis caused by Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, pathogenic Staphylococci and other Streptococci. Cows at the first calving had a 1.38–1.65-times higher risk of having stillborn calves than cows of parity ≥ 2. From a practical point, veterinarians and farmers can consider the effect of subclinical mastitis during late gestation on the risk of stillbirth and it could help for strategies of optimizing reproductive performance in dairy cows.
Adaptive Responses of Thyroid Hormones, Insulin, and Glucose during Pregnancy and Lactation in Dairy Cows
Authors: Fazio Esterina , Bionda Arianna , Chiofalo Vincenzo , Crepaldi Paola , Lopreiato Vincenzo , Medica Pietro , Liotta Luigi
Published: May 2022

The study examined the effects of different stages of pregnancy and lactation on thyroid, insulin, and glucose responses in dairy cows. In the present study, 30 dairy cows (10 Holstein, 10 Simmental, and 10 Brown) at 30 ± 20 d of lactation were randomly selected and blood samples were collected once every 60 d for one year to measure circulating thyroid stimulating hormone (TSH), total and free triiodothyronines (T3, fT3) and thyroxines (T4, fT4), insulin, and glucose. Pregnant cows showed higher T4 (p = 0.010) and insulin (p = 0.046) concentrations at >180 d than at >60–120 d of pregnancy and in nonpregnant cows. Along the lactation phase, circulating T4 concentrations showed a biphasic trend, decreasing from 0–60 d to >60–120 d phase, which showed the lowest values, and then increasing until the end of lactation (>300 d) (p = 0.016). Glucose showed the highest concentrations at the start of lactation (0–120 days) and the lowest values at the end (p = 0.008). The monitoring of thyroid hormones, insulin, and glucose changes represents an important tool to evaluate the anabolic and/or catabolic adaptation in response to functional periods in dairy cows, which can potentially predispose the cows to an excessive negative energy balance and related metabolic issues.
Reducing the Fermentability of Wheat with a Starch Binding Agent Reduces Some of the Negative Effects of Heat Stress in Sheep
Authors: Prathap Pragna , Chauhan Surinder S. , Leury Brian J. , Cottrell Jeremy J. , Joy Aleena , Zhang Minghao , Dunshea Frank R.
Published: May 2022

The objective of this study was to investigate the effects of reducing the fermentability of grains on thermoregulatory responses in heat stressed (HS) lambs. To achieve this, wheat grain treated with a commercial starch binding agent, Bioprotect, is compared to maize, which has already demonstrated effects in ameliorating heat stress-induced thermoregulation responses and untreated wheat grains. An initial in vitro experiment was conducted to examine cumulative gas production from the fermentation of wheat grain with different dosages of the commercial starch binding agent, Bioprotect. Based on the in vitro results, an in vivo lamb experiment was conducted using 24 Merino lambs (1 year old; 42.6 ± 3.6 kg BW). The lambs were offered one of three dietary treatments: a wheat-based diet (WD), a Bioprotect treated wheat-based diet (BD), and a maize-based diet (MD). Three successive 1-week experimental periods were conducted with lambs from all dietary groups (P1, P2, and P3). During P1, lambs were exposed to a TN environment and fed a 1.7× Maintenance feed intake (MF) level; in P2, lambs were kept in a HS environment and fed a 1.7× MF level; and in P3, animals were kept in a HS environment and fed a 2× MF level. The in vitro experiment revealed a reduction in cumulative gas production (p < 0.05) from the Bioprotect treated wheat compared to untreated wheat samples. In the in vivo component of the study, the replacement of wheat with maize or 2% Bioprotect-treated wheat reduced the respiration rate (p < 0.001) and heart rate (p ≤ 0.01) of lambs during HS. There was a reduction in the concentration of blood gas variables such as a base excess of blood (BE(b)) and extracellular fluid (BE(ecf)), bicarbonate (CHCO3−), the partial pressure of carbon dioxide (pCO2), the total concentration of carbon dioxide (ctCO2), and sodium (Na+) (p ≤ 0.001 for all) during the periods of HS compared to the thermoneutral conditions. Moreover, BD- and MD-fed lambs had a higher blood potassium concentration (K+) than the WD-fed lambs (p = 0.008). The results of the present study suggest that Bioprotect can be a viable feed treatment strategy for treating rapidly fermentable grains such as wheat to alleviate the effects of HS. Further, Bioprotect-treated wheat could be an option to replace maize in concentrate rations in jurisdictions where maize is cost-prohibitive or unavailable.
Effect of Dietary Supplementation with Mannose Oligosaccharides on the Body Condition, Lactation Performance and Their Offspring of Heat-Stressed Sows
Authors: Ren Ying , Zheng Zibin , Wu Taotao , Lei Long , Liu Zhengya , Zhao Yuanqi , Zhao Shengjun
Published: May 2022

The aim of this study was to determine the effects of dietary supplementation with mannose oligosaccharide (MOS) on the condition of the body and the reproductive and lactation performances of sows. Eighty pregnant sows were randomly assigned to four groups with a 2 × 2 factorial design: with or without MOS (1 g/kg) and with or without heat stress (HS) challenge. The temperature in the HS groups (HS and HM group) was controlled at 31.56 ± 1.22 °C, while the temperature in the active cooling (AC) groups (AC and AM group) was controlled at 23.49 ± 0.72 °C. The weight loss of sows in the AC group was significantly lower than that of sows in the HS group (p < 0.01). The weight and backfat thickness loss of sows supplemented with MOS displayed a downward trend. The average birth weight of the litter significantly increased in the HM group (basic diet + MOS) compared with the HS group (p < 0.05). The milk protein of sows significantly decreased under the HS condition at 2 and 12 h after delivery (p < 0.05). However, the milk immunoglobin G (IgG) of sows in the HS group increased significantly compared with that of sows in the HM group (p < 0.05) at 12 and 24 h after delivery. The levels of serum urea nitrogen (UREA) and glucose (GLU) decreased significantly under the HS condition (p < 0.05), while the level of interleukin-6 (IL-6) increased significantly under the HS condition (p < 0.05). Dietary supplementation with MOS also significantly reduced TNF-α under the AC conditions (p < 0.05). In conclusion, HS significantly affected the body condition, lactation performances and their offspring of sows. However, dietary supplementation with 1 g/kg MOS did not result in statistically significant changes.
Effects of Concentrate Supplementation on Growth Performance, Rumen Fermentation, and Bacterial Community Composition in Grazing Yaks during the Warm Season
Authors: Dai Dongwen , Pang Kaiyue , Liu Shujie , Wang Xun , Yang Yingkui , Chai Shatuo , Wang Shuxiang
Published: May 2022

This study aimed to evaluate the effects of concentrate supplementation on the growth performance, serum biochemical parameters, rumen fermentation, and bacterial community composition of grazing yaks during the warm season. Eight male yaks (body weight, 123.96 ± 7.43 kg; 3-years) were randomly allocated to two treatments groups: grazing (n = 4, GY) and concentrate supplement group (n = 4, GYS). Concentrate supplementation increased the average daily gain (ADG) (p < 0.05). Glucose (GLU), total protein (TP), and aspartate aminotransferase (AST) serum concentrations were significantly higher in the GYS group than in the GY group (p < 0.05). Ammonia-N, MCP: microbial protein, and total volatile fatty acid concentrations were significantly higher in the GYS group than in the GY group (p < 0.01), whereas the pH and acetate: propionate values were significantly decreased (p < 0.01). The relative abundance of Firmicutes in the rumen fluid was significantly higher in the GYS group than in the GY group (p < 0.01). At the genus level, the relative abundances of Succiniclasticum, Prevotellaceae_UCG_003, Prevotellaceae_UCG_005, and Ruminococcus_1 were significantly greater in the GY group than in the GYS group (p < 0.01). In conclusion, concentrate supplementation improved yaks’ growth potential during the warm season, improved ruminal fermentation, and altered core bacteria abundance.
Genome-Wide DNA Methylation Patterns of Muscle and Tail-Fat in DairyMeade Sheep and Mongolian Sheep
Authors: Luo Rongsong , Dai Xuelei , Zhang Li , Li Guangpeng , Zheng Zhong
Published: May 2022

This study aimed to explore the genome-wide DNA methylation differences between muscle and tail-fat tissues of DairyMeade sheep (thin-tailed, lean carcass) and Mongolian sheep (fat-tailed, fat-deposited carcass). Whole-genome bisulfite sequencing (WGBS) was conducted and the global DNA methylation dynamics were mapped. Generally, CGs had a higher DNA methylation level than CHHs and CHGs, and tail-fat tissues had higher CG methylation levels than muscle tissues. For DNA repeat elements, SINE had the highest methylation level, while Simple had the lowest. When dividing the gene promoter region into small bins (200 bp per bin), the bins near the transcription start site (±200 bp) had the highest CG count per bin but the lowest DNA methylation levels. A series of DMRs were identified in muscle and tail-fat tissues between the two breeds. Among them, the introns of gene CAMK2D (calcium/calmodulin-dependent protein kinase II δ) demonstrated significant DNA methylation level differences between the two breeds in both muscle and tail-fat tissues, and it may play a crucial role in fat metabolism and meat quality traits. This study may provide basic datasets and references for further epigenetic modification studies during sheep genetic improvement.
Antimicrobial Peptides (AMPs) in the Pathogenesis of Alzheimer’s Disease: Implications for Diagnosis and Treatment
Authors: Bruno Francesco , Malvaso Antonio , Canterini Sonia , Bruni Amalia Cecilia
Published: May 2022

Alzheimer’s disease (AD) represents the most frequent type of dementia in elderly people. There are two major forms of the disease: sporadic (SAD)—whose causes are not completely understood—and familial (FAD)—with clear autosomal dominant inheritance. The two main hallmarks of AD are extracellular deposits of amyloid-beta (Aβ) peptide and intracellular deposits of the hyperphosphorylated form of the tau protein (P-tau). An ever-growing body of research supports the infectious hypothesis of sporadic forms of AD. Indeed, it has been documented that some pathogens, such as herpesviruses and certain bacterial species, are commonly present in AD patients, prompting recent clinical research to focus on the characterization of antimicrobial peptides (AMPs) in this pathology. The literature also demonstrates that Aβ can be considered itself as an AMP; thus, representing a type of innate immune defense peptide that protects the host against a variety of pathogens. Beyond Aβ, other proteins with antimicrobial activity, such as lactoferrin, defensins, cystatins, thymosin β4, LL37, histatin 1, and statherin have been shown to be involved in AD. Here, we summarized and discussed these findings and explored the diagnostic and therapeutic potential of AMPs in AD.
Evidence on the Use of Mouthwash for the Control of Supragingival Biofilm and Its Potential Adverse Effects
Authors: Takenaka Shoji , Sotozono Maki , Ohkura Naoto , Noiri Yuichiro
Published: May 2022

Antimicrobial mouthwash improves supragingival biofilm control when used in conjunction with mechanical removal as part of an oral hygiene routine. Mouthwash is intended to suppress bacterial adhesion during biofilm formation processes and is not aimed at mature biofilms. The most common evidence-based effects of mouthwash on the subgingival biofilm include the inhibition of biofilm accumulation and its anti-gingivitis property, followed by its cariostatic activities. There has been no significant change in the strength of the evidence over the last decade. A strategy for biofilm control that relies on the elimination of bacteria may cause a variety of side effects. The exposure of mature oral biofilms to mouthwash is associated with several possible adverse reactions, such as the emergence of resistant strains, the effects of the residual structure, enhanced pathogenicity following retarded penetration, and ecological changes to the microbiota. These concerns require further elucidation. This review aims to reconfirm the intended effects of mouthwash on oral biofilm control by summarizing systematic reviews from the last decade and to discuss the limitations of mouthwash and potential adverse reactions to its use. In the future, the strategy for oral biofilm control may shift to reducing the biofilm by detaching it or modulating its quality, rather than eliminating it, to preserve the benefits of the normal resident oral microflora.