bluuebunny/crossref_metadata_2025 huggingface dataset:
Discovery of genomic regions associated with grain yield and agronomic traits in Bi-parental populations of maize (Zea mays. L) Under optimum and low nitrogen conditions
Authors: Kimutai Collins , Ndlovu Noel , Chaikam Vijay , Ertiro Berhanu Tadesse , Das Biswanath , Beyene Yoseph , Kiplagat Oliver , Spillane Charles , Prasanna Boddupalli M. , Gowda Manje
Published: October 2023

Low soil nitrogen levels, compounded by the high costs associated with nitrogen supplementation through fertilizers, significantly contribute to food insecurity, malnutrition, and rural poverty in maize-dependent smallholder communities of sub-Saharan Africa (SSA). The discovery of genomic regions associated with low nitrogen tolerance in maize can enhance selection efficiency and facilitate the development of improved varieties. To elucidate the genetic architecture of grain yield (GY) and its associated traits (anthesis-silking interval (ASI), anthesis date (AD), plant height (PH), ear position (EPO), and ear height (EH)) under different soil nitrogen regimes, four F3 maize populations were evaluated in Kenya and Zimbabwe. GY and all the traits evaluated showed significant genotypic variance and moderate heritability under both optimum and low nitrogen stress conditions. A total of 91 quantitative trait loci (QTL) related to GY (11) and other secondary traits (AD (26), PH (19), EH (24), EPO (7) and ASI (4)) were detected. Under low soil nitrogen conditions, PH and ASI had the highest number of QTLs. Furthermore, some common QTLs were identified between secondary traits under both nitrogen regimes. These QTLs are of significant value for further validation and possible rapid introgression into maize populations using marker-assisted selection. Identification of many QTL with minor effects indicates genomic selection (GS) is more appropriate for their improvement. Genomic prediction within each population revealed low to moderately high accuracy under optimum and low soil N stress management. However, the accuracies were higher for GY, PH and EH under optimum compared to low soil N stress. Our findings indicate that genetic gain can be improved in maize breeding for low N stress tolerance by using GS.
Identification of the immune-associated characteristics and predictive biomarkers of keratoconus based on single-cell RNA-sequencing and bulk RNA-sequencing
Authors: Niu Xiaoguang , Xu Man , Zhu Jian , Zhang Shaowei , Yang Yanning
Published: October 2023

Background

Whether keratoconus (KC) is an inflammatory disease is currently debated. Hence, we aimed to investigate the immune-related features of KC based on single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing (bulk RNA-seq) data.

Methods

scRNA-seq data were obtained from the Genome Sequence Archive (GSA), bulk RNA-seq data were obtained from the Gene Expression Omnibus (GEO), and immune-associated genes(IAGs) were obtained from the ImmPort database. Cell clusters of KC were annotated, and different cell clusters were then selected. The IAG score of each cell was calculated using the AUCell package. Three bulk RNA-seq datasets were merged and used to identify the differentially expressed genes (DEGs), biological functions, and immune characteristics. Weighted gene coexpression network analysis (WGCNA) was used to select the IAG score-related hub genes. Based on scRNA-seq and bulk RNA-seq analyses, three machine learning algorithms, including random forest (RF), support vector machine (SVM), and least absolute shrinkage and selection operator (LASSO) regression analysis, were used to identify potential prognostic markers for KC. A predictive nomogram was developed based on prognostic markers.

Results

Six cell clusters were identified in KC, and decreased corneal stromal cell-5 (CSC-5) and increased CSC-6 were found in KC. CSC and immune cell clusters had the highest IAG scores. The bulk RNA-seq analysis identified 1362 DEGs (553 upregulated and 809 downregulated) in KC. We found different immune cell populations and differentially expressed cytokines in KC. More than three key IAG score-related modules and 367 genes were identified. By integrating the scRNA-seq and bulk RNA-seq analyses, 250 IAGs were selected and then incorporated into three machine learning models, and 10 IAGs (CEP112, FYN, IFITM1, IGFBP5, LPIN2, MAP1B, RNASE1, RUNX3, SMIM10, and SRGN) were identified as potential prognostic genes that were significantly associated with cytokine and matrix metalloproteinase(MMP)1-14 expression. Finally, a predictive nomogram was constructed and validated.

Conclusion

Taken together, our results identified CSCs and immune cell clusters that may play a key role during KC progression by regulating immunological features and maintaining cell stability.
Modulating asthma–COPD overlap responses with IL-17 inhibition
Authors: Camargo Leandro do Nascimento , Righetti Renato Fraga , de Almeida Francine Maria , Santos Tabata Maruyama dos , Fukuzaki Silvia , Martins Nilo Arthur Bezerra , Barbeiro Miguel Cantadori , Saraiva-Romanholo Beatriz Mangueira , Lopes Fernanda Degobbi Tenorio Quirino dos Santos , Leick Edna Aparecida , Prado Carla Máximo , Tibério Iolanda de Fátima Lopes Calvo
Published: October 2023

Background

IL-17 is a modulator of the inflammatory response and is implicated in lung remodeling in both asthma and chronic obstructive pulmonary disease (COPD). Well as and probably in patients with asthma–COPD overlap (ACO).

Methods

In this study, we evaluated the response of the airways and alveolar septa to anti-IL-17 treatment in an ACO model. Fifty-six male BALB/c mice were sensitized with ovalbumin (OVA group), received porcine pancreatic elastase (PPE group), or both (ACO group). Mice were then treated with either anti-IL-17 monoclonal antibody or saline. We evaluated hyperresponsiveness, bronchoalveolar lavage fluid (BALF) cell counts, and mean alveolar diameter. We quantified inflammatory, response, extracellular matrix remodeling, oxidative stress markers, and signaling pathway markers.

Results

Anti-IL-17 treatment in the ACO anti-IL-17 group reduced the maximum response of respiratory system Rrs, Ers, Raw, Gtis, this when compared to the ACO group (p<0.05). There was a reduction in the total number of inflammatory cells, neutrophils, and macrophages in the BALF in the ACO anti-IL-17 group compared to the ACO group (p<0.05). There was attenuated dendritic cells, CD4+, CD8+, FOXP3, IL-1β, IL-2, IL-6, IL-13, IL-17, IL-33 in ACO anti-IL-17 group in airway and alveolar septum compared to the ACO group (p<0.05). We observed a reduction of MMP-9, MMP-12, TIMP-1, TGF-β, collagen type I in ACO anti-IL-17 group in airway and alveolar septum compared to the ACO group (p < 0.05). We also observed a reduction of iNOS and 8-iso-PGF2α in the airways and in the alveolar septum was reduced in the ACO anti-IL-17group compared to the ACO group (p < 0.05). Regarding the signaling pathways, NF-kB, ROCK-1, and ROCK-2 in the airway and alveolar septum were attenuated in the ACO anti-IL-17 group when compared to the ACO group (p<0.05).

Conclusions

Our results suggest that inhibiting IL-17 modulates cell-associated cytokine production in lung tissue, extracellular matrix remodeling, and oxidative stress in ACO through the modulation of NF-kB and FOXP3.
Association between the β-blocker use and patients with sepsis: a cohort study
Authors: Yang Qilin , Kong Tianyu , Bao Ziping , Yang Shanshan , Chen Xiaohua , Zheng Jiezhao , Xiong Xuming , Wen Deliang , Zhang Zhenhui
Published: October 2023

Objective

This study aimed to assess whether β-blockers are associated with mortality in patients with sepsis.

Method

We conducted a retrospective cohort study of patients with sepsis using the Medical Information Market for Intensive Care (MIMIC)-IV and the emergency intensive care unit (eICU) databases. The primary outcome was the in-hospital mortality rate. The propensity score matching (PSM) method was adopted to reduce confounder bias. Subgroup and sensitivity analyses were performed to test the stability of the conclusions.

Results

We included a total of 61,751 patients with sepsis, with an overall in-hospital mortality rate of 15.3% in MIMIC-IV and 13.6% in eICU. The inverse probability-weighting model showed that in-hospital mortality was significantly lower in the β-blockers group than in the non-β-blockers group [HR = 0.71, 95% CI: 0.66–0.75, p < 0.001 in MIMIC-IV, and HR = 0.48, 95% CI: 0.45–0.52, p < 0.001 in eICU]. In subgroups grouped according to sex, age, heart rate, APSIII, septic shock, and admission years, the results did not change.

Conclusion

β-blocker use is associated with lower in-hospital mortality in patients with sepsis, further randomized trials are required to confirm this association.
Effects of wearing masks during COVID-19 pandemic on the composition and diversity of skin bacteria and fungi in medical workers
Authors: Zhang Jingxi , Jiang Peiyun , Zhang Yaxin , Liu Wenlou , Kong Shujing , Hou Xiaoyang , Qi Zuoyao , Sun Yujin , Jiang Guan
Published: October 2023

Background

Although studies have shown that wearing masks can affect the skin microbiome, more detailed and comprehensive research on wearing masks needs to be further explored.

Objective

This study aimed to characterize the influence of mask wearing on the diversity and structural characteristics of the facial skin microbial community of medical staff during the COVID-19 pandemic by means of metagenomic sequencing (mNGS).

Methods

A total of 40 samples were taken by swabbing the cheek in the 2 × 2 cm2 area before and after wearing the masks. DNA was extracted for metagenomic sequencing.

Results

A statistically significant decrease was found in the α diversity between BN and AN groups and between B2 h and A2 h groups. BN and AN mean groups before and after 8 h of wearing the medical protective mask (N95), including 10 volunteers, respectively. B2 h and A2 h mean groups before and after 8 h of wearing masks, including 10 volunteers changing mask every 2 h, respectively. The β diversity was found to be statistically reduced between BS and AS groups (p = 0.025), BN and AN groups (p = 0.009), and B2 h and A2 h group (p = 0.042). The fungal beta diversity was significantly decreased in every group before and after wearing masks. The main bacteria on the face before and after wearing masks were Cutibacterium (68.02 and 71.73%). Among the fungi, Malassezia predominated the facial skin surface before and after wearing masks (35.81 and 39.63%, respectively).

Conclusion

Wearing different types of masks and changing masks according to different frequency will have different effects on the facial skin’s microbiota.
Children with developmental coordination disorder are less able to fine-tune muscle activity in anticipation of postural perturbations than typically developing counterparts
Authors: Harkness-Armstrong Carla , Hodson-Tole Emma F. , Wood Greg , Mills Richard
Published: October 2023

The majority of children with developmental coordination disorder (DCD) struggle with static and dynamic balance, yet there is limited understanding of the underlying neuromechanical mechanisms that underpin poor balance control in these children. Eighteen children with DCD and seven typically developing (TD) children aged 7–10 years stood with eyes open on a moveable platform progressively translated antero-posteriorly through three frequencies (0.1, 0.25 and 0.5 Hz). Myoelectric activity of eight leg muscles, whole-body 3D kinematics and centre of pressure were recorded. At each frequency, postural data were divided into transition-state and steady-state cycles. Data were analyzed using a linear mixed model with follow-up Tukey’s pairwise comparisons. At the slowest frequency, children with DCD behaved like age-matched TD controls. At the fastest frequency, children with DCD took a greater number of steps, had a greater centre of mass variability, had a greater centre of pressure area, and tended to activate their muscles earlier and for longer than TD children. Children with DCD did not alter their postural response following prolonged exposure to platform movement, however they made more, non-structured postural adjustments in the medio-lateral direction as task difficulty increased. At the faster oscillation frequencies, children with DCD adopted a different muscle recruitment strategy to TD children. Activating their muscles earlier and for longer may suggest that children with DCD attempt to predict and react to postural disturbances, however the resulting anticipatory muscle excitation patterns do not seem as finely tuned to the perturbation as those demonstrated by TD children. Future work should examine the impact of balance training interventions on the muscle recruitment strategies of children with DCD, to ensure optimal interventions can be prescribed.
Role of mitophagy in ocular neurodegeneration
Authors: Brooks Calvin D. , Kodati Bindu , Stankowska Dorota L. , Krishnamoorthy Raghu R.
Published: October 2023

Neurons in the central nervous system are among the most metabolically active cells in the body, characterized by high oxygen consumption utilizing glucose both aerobically and anaerobically. Neurons have an abundance of mitochondria which generate adequate ATP to keep up with the high metabolic demand. One consequence of the oxidative phosphorylation mechanism of ATP synthesis, is the generation of reactive oxygen species which produces cellular injury as well as damage to mitochondria. Mitochondria respond to injury by fusion which serves to ameliorate the damage through genetic complementation. Mitochondria also undergo fission to meet an increased energy demand. Loss of mitochondria is also compensated by increased biogenesis to generate new mitochondria. Damaged mitochondria are removed by mitophagy, an autophagic process, in which damaged mitochondria are surrounded by a membrane to form an autophagosome which ultimately fuses with the lysosome resulting in degradation of faulty mitochondria. Dysregulation of mitophagy has been reported in several central nervous system disorders, including, Alzheimer’s disease and Parkinson’s disease. Recent studies point to aberrant mitophagy in ocular neurodegenerative disorders which could be an important contributor to the disease etiology/pathology. This review article highlights some of the recent findings that point to dysregulation of mitophagy and it’s underlying mechanisms in ocular neurodegenerative diseases, including, glaucoma, age-related macular degeneration and diabetic retinopathy.
Characteristics of cancer-related fatigue and its correlation with anxiety, depression, and stress-related hormones among Chinese cancer survivors: a cross-sectional study
Authors: Gu Shanshan , Xu Yun , Zhu Xiaoshu , Lam Anderson , Yi Danhui , Gong Lutian , Wang Jinghui , Guo Xinyu , Fu Li , Shi Jiyan , Wang Feiye , Liu Ketan
Published: October 2023

Background

Fatigue is a common source of distress for cancer survivors. The severity of cancer-related fatigue varies significantly, which may be due to individual differences in host factors.

Aim

This cross-sectional study aims to explore how demographic, oncological, sociological, psychological, and stress-related hormones levels interact to influence the distinct experiences of fatigue (Cancer-related fatigue [CRF] occurrence and fatigue degree).

Methods

A cross-sectional study carried out at the oncology outpatient and ward department of Xiyuan Hospital of China Academy of Chinese Medical Sciences recruited 306 cancer patients between January 2021 to December 2021. General information, fatigue, psychological factors was evaluated by general information questionnaire, the Revised Piper’s Fatigue Scale-Chinese Version (RPFS-CV), and the self-report Hospital Anxiety and Depression Scale (HADS). Stress-related hormones were measured with chemiluminescent enzyme immunoassay (Zhengzhou Antobio).

Results

306 patients were included, 229 (74.8%) were diagnosed with CRF, including 94 (41.0%) with mild fatigue, 121 (52.8%) with moderate fatigue, and 14 (6.1%) with severe fatigue. Multivariate regression analysis showed that higher depression scores, aldosterone levels may increase the risk of CRF. Patients who are obese (Body mass index ≥ 28 kg/m2) may help to reduce the risk of CRF. Other contributing factors for increased levels of fatigue (p< 0.05) include being female, having anxiety, depression and high aldosterone levels.

Conclusion

The research suggested that CRF was a common symptom in cancer survivors and pay attention to these influencing factors may help to better identify patients susceptible to fatigue and provide long-term, targeted interventions.
Genome editing for healthy crops: traits, tools and impacts
Authors: Yıldırım Kubilay , Miladinović Dragana , Sweet Jeremy , Akin Meleksen , Galović Vladislava , Kavas Musa , Zlatković Milica , de Andrade Eugenia
Published: October 2023

Crop cultivars in commercial use have often been selected because they show high levels of resistance to pathogens. However, widespread cultivation of these crops for many years in the environments favorable to a pathogen requires durable forms of resistance to maintain “healthy crops”. Breeding of new varieties tolerant/resistant to biotic stresses by incorporating genetic components related to durable resistance, developing new breeding methods and new active molecules, and improving the Integrated Pest Management strategies have been of great value, but their effectiveness is being challenged by the newly emerging diseases and the rapid change of pathogens due to climatic changes. Genome editing has provided new tools and methods to characterize defense-related genes in crops and improve crop resilience to disease pathogens providing improved food security and future sustainable agricultural systems. In this review, we discuss the principal traits, tools and impacts of utilizing genome editing techniques for achieving of durable resilience and a “healthy plants” concept.
Phylogenetic analysis of endogenous viral elements in the rice genome reveals local chromosomal evolution in Oryza AA-genome species
Authors: Saito Nozomi , Chen Sunlu , Kitajima Katsuya , Zhou Zhitong , Koide Yohei , Encabo Jaymee R. , Diaz Maria Genaleen Q. , Choi Il-Ryong , Koyanagi Kanako O. , Kishima Yuji
Published: October 2023

Introduction

Rice genomes contain endogenous viral elements homologous to rice tungro bacilliform virus (RTBV) from the pararetrovirus family Caulimoviridae. These viral elements, known as endogenous RTBV-like sequences (eRTBVLs), comprise five subfamilies, eRTBVL-A, -B, -C, -D, and -X. Four subfamilies (A, B, C, and X) are present to a limited degree in the genomes of the Asian cultivated rice Oryza sativa (spp. japonica and indica) and the closely related wild species Oryza rufipogon.

Methods

The eRTBVL-D sequences are widely distributed within these and other Oryza AA-genome species. Fifteen eRTBVL-D segments identified in the japonica (Nipponbare) genome occur mostly at orthologous chromosomal positions in other AA-genome species. The eRTBVL-D sequences were inserted into the genomes just before speciation of the AA-genome species.

Results and discussion

Ten eRTBVL-D segments are located at six loci, which were used for our evolutionary analyses during the speciation of the AA-genome species. The degree of genetic differentiation varied among the eRTBVL-D segments. Of the six loci, three showed phylogenetic trees consistent with the standard speciation pattern (SSP) of the AA-genome species (Type A), and the other three represented phylogenies different from the SSP (Type B). The atypical phylogenetic trees for the Type B loci revealed chromosome region–specific evolution among the AA-genome species that is associated with phylogenetic incongruences: complex genome rearrangements between eRTBVL-D segments, an introgression between the distant species, and low genetic diversity of a shared eRTBVL-D segment. Using eRTBVL-D as an indicator, this study revealed the phylogenetic incongruence of local chromosomal regions with different topologies that developed during speciation.
International comparative study of learning trajectories based on TIMSS 2019 G4 data on cognitive diagnostic models
Authors: Zhu Zhemin
Published: October 2023

Learning trajectory describes the student’s learning progress or steps in one area through which teaching and learning can be linked. The cognitive diagnostic model (CDM) is an emerging evaluation theory in education measures. Researchers can determine students’ mastery of fine-grained knowledge points by describing the learning trajectory based on CDM. The present study is based on the Trends in International Mathematics and Science Study (TIMSS) 2019, particularly, 21 mathematical items in the fourth grade. It analyzes the response data of 2,854 students from 17 countries from 10 attributes based on CDM. This study explores students’ different learning trajectories in the content field by analyzing the relationships between knowledge states and attribute patterns. The study found that the 17 countries differ in learning trajectories but have commonalities. The learning starting points of 17 countries can be roughly divided into two categories and geometry attribute and fractions and decimals attribute are the last two attributes to master.
An empirical analysis of the factors driving customers’ purchase intention of green smart home products
Authors: Guo Mingyan , Xiao Shufeng (Simon)
Published: October 2023

With the improvement of consumers’ environmental awareness and the popularity of the Internet of Things, green smart home products (GSHPs) are becoming the dominant trend of future home life. This shift not only makes tedious home life easier and more convenient but also helps families save energy and reduce carbon emissions. However, given the impact of the current technological level, the proportion of users who actually purchase GSHPs remains small. Thus, seeking ways to promote the consumption of GSHPs has become an urgent issue. Hence, this study seeks to fill the gap in the existing research on green consumption behavior and obtain a full understanding of the factors influencing the purchase intention of GSHPs. To do so, this work uses task-technology fit theory and considers the actual situation of green smart home consumption to add social-technology fit into the original theoretical basis. In particular, this research focuses on middle- and high-end Chinese consumers who have experience in purchasing GSHPs. Moreover, it aims for an in-depth exploration of the formation mechanism of Chinese consumers’ purchase intention for GSHPs through structural equation modeling. Using survey data collected from 331 green smart home product users in China, the study empirically examines the relationships among autonomy, environmental agility, sense of belonging, and self-actualization, and both task-technology fit and social-technology fit, which are expected to shape the purchase intention of GSHP users. The empirical results provide broad support for our hypotheses. The results of this study offer important contributions to the increasing research on GSHPs consumption and shed light on the importance of both technology characteristics and the needs of users in achieving both task-technology fit and social-technology fit and, ultimately enhancing the users’ intention to purchase GSHPs.
A mixed method examination: how stigma experienced by autistic adults relates to metrics of social identity and social functioning
Authors: Marion Alex , Bowman Karrah , Thomas Gina , Harrison Ashley J.
Published: October 2023

A recent meta-analysis reveals almost half of autistic individuals experience some form of victimization in their lifetime, including bullying and other forms of stigma. Research among caregivers of autistic individuals demonstrates that stigma can have a long-lasting impact on other aspects of a social identity, such as self-esteem, but less research has specifically examined this among autistic adults themselves, in spite of research suggesting these are likely constructs that contribute to the internalization of stigma and subsequent mental health consequences. The current study used a mixed method approach to assess the relation between stigma and several components of social identity and social functioning. More specifically, among 45 autistic young adults, three dimensions of self-reported stigma (discrimination, disclosure, and positive aspects) were examined in relation to self-esteem, self-efficacy, social satisfaction and adaptive social functioning. Quantitative analyses revealed higher reported discriminative and disclosure stigma were significantly associated with lower self-efficacy. Increased experience with all types of stigma were associated with lower social satisfaction. Greater reported disclosure stigma was also associated with lower self-esteem. Qualitative interviewing among eight autistic young adults helped to better understand the nature of stigma and the impact of these experiences. Thematic analysis of the qualitative data revealed that all of the participants experienced stigma in the form of exclusion or isolation and that a majority also experienced verbal bullying. Many of the negative interactions came from educators, peers, and family members. Most participants indicated that these stigmatizing interactions directly contributed to decreased social satisfaction, diminished self-efficacy, and lowered self-esteem. A greater understanding of the negative consequences of stigma can inform efforts to increase awareness and acceptance of autism.
A mixed method comparison of stigma toward autism and schizophrenia and effects of person-first versus identity-first language
Authors: Jones Desiree R. , Sasson Noah J.
Published: October 2023

Introduction

While stigma toward autistic individuals has been well documented, less is known about how autism is perceived relative to other stigmatized disabilities. As a highly stigmatized condition with similar social cognitive features to autism, schizophrenia may offer a useful comparison for stigma. Previous studies have found that autistic people may be perceived more favorably than those with schizophrenia, but little is known about the underlying volitional thoughts that contribute to differences in how these conditions are perceived.

Methods

The present study utilizes a mixed-methods approach, allowing for a detailed understanding of how young adults perceive different diagnostic labels. 533 college undergraduates completed questionnaires reflecting their perceptions of one of eight diagnostic labels: four related to autism (autism, autistic, autism spectrum disorder, or Asperger’s), two related to schizophrenia (schizophrenia or schizophrenic), and two related to an unspecified clinical condition (clinical diagnosis or clinical disorder). Participants also completed an open-ended question regarding their thoughts about, and exposure to, these labels. Responses were compared across broader diagnostic categories (autism, schizophrenia, general clinical condition), with thematic analysis used to assess the broader themes occurring within the open-ended text.

Results

While perceptions did not differ significantly for person-first and identity-first language within labels, several differences were apparent across labels. Specifically, quantitative results indicated greater prejudice towards autism and schizophrenia than the generic clinical condition, with schizophrenia associated with more perceived fear and danger, as well as an increased preference for social distance, compared to autism. Patterns in initial codes differed across diagnostic labels, with greater variation in responses about autism than responses about schizophrenia or the general clinical condition. While participants described a range of attitudes toward autism (patronizing, exclusionary, and accepting) and schizophrenia (fear, prejudice, and empathy), they refrained from describing their attitudes toward the general clinical label, highlighting the centrality of a cohesive group identity for the development of stigma. Finally, participants reported a number of misconceptions about autism and schizophrenia, with many believing features such as savant syndrome to be core characteristics of the conditions.

Conclusion

These findings offer a more detailed account of how non-autistic individuals view autism and may therefore aid in the development of targeted programs to improve attitudes toward autism.
Droplet digital PCR application for the detection of SARS-CoV-2 in air sample
Authors: Rashid Siti Aishah , Nazakat Raheel , Muhamad Robat Rosnawati , Ismail Rohaida , Suppiah Jeyanthi , Rajendran Kamesh , Raj Louis Masalamany A. S. Santhana , Muhamad Hendri Nur Afrina , Mohamad Nadia , Khairul Hasni Nurul Amalina , Suib Fatin Amirah , Nik Hassan Nik Muhamad Nizam , Pahrol Muhammad Alfatih , Shaharudin Rafiza
Published: October 2023

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) may transmit through airborne route particularly when the aerosol particles remain in enclosed spaces with inadequate ventilation. There has been no standard recommended method of determining the virus in air due to limitations in pre-analytical and technical aspects. Furthermore, the presence of low virus loads in air samples could result in false negatives. Our study aims to explore the feasibility of detecting SARS-CoV-2 ribonucleic acid (RNA) in air samples using droplet digital polymerase chain reaction (ddPCR). Active and passive air sampling was conducted between December 2021 and February 2022 with the presence of COVID-19 confirmed cases in two hospitals and a quarantine center in Klang Valley, Malaysia. SARS-CoV-2 RNA in air was detected and quantified using ddPCR and real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The comparability of two different digital PCR platforms (QX200 and QIAcuity) to RT-PCR were also investigated. Additionally negative staining transmission electron microscopy was performed to visualize virus ultrastructure. Detection rates of SARS-CoV-2 in air samples using ddPCR were higher compared to RT-PCR, which were 15.2% (22/145) and 3.4% (5/145), respectively. The sensitivity and specificity of ddPCR was 100 and 87%, respectively. After excluding 17 negative samples (50%) by both QX200 and QIAcuity, 15% samples (5/34) were found to be positive both ddPCR and dPCR. There were 23.5% (8/34) samples that were detected positive by ddPCR but negative by dPCR. In contrast, there were 11.7% (4/34) samples that were detected positive by dPCR but negative by ddPCR. The SARS-CoV-2 detection method by ddPCR is precise and has a high sensitivity for viral RNA detection. It could provide advances in determining low viral titter in air samples to reduce false negative reports, which could complement detection by RT-PCR.
Progress in studying the impact of hyperlipidemia and statins on rotator cuff injury and repair
Authors: Qian Yinhua , Huang Haoqiang , Wan Renwen , Zhou Yu , Feng Xinting , Xu Feng , Luo Zhiwen , Wang Qing
Published: October 2023

This review delves into the intersection of two prevalent conditions, hyperlipidemia and rotator cuff injuries, both of which bear substantial healthcare burdens. Our investigation begins with an exploration of rotator cuff injuries, common musculoskeletal disorders that severely impair shoulder functionality and quality of life. These injuries are notably pervasive among sports enthusiasts and the older adult, with an incidence rate estimated at 5–10% in the general population. Despite their widespread occurrence and the diverse, multifactorial etiological factors, effective treatment strategies remain elusive. We then examine hyperlipidemia, a metabolic disorder affecting approximately 40% of the global adult population. Characterized by elevated levels of cholesterol and triglycerides, hyperlipidemia can precipitate severe cardiovascular complications and presents a significant socioeconomic burden. Although current management strategies encompass lifestyle modifications and pharmacological interventions, the condition remains a formidable health challenge. Central to this review is the exploration of a potential association between hyperlipidemia and rotator cuff injuries. We aim to synthesize the current understanding of hyperlipidemia’s role in the pathophysiology of rotator cuff injuries, thereby offering fresh insights into their common etiological underpinnings, potential therapeutic targets, and drugs, such as Statins. The influence of other lipid-lowering therapeutics on tendon health is also considered, and further research into the molecular pathways and potential therapeutic benefits of these drugs is required. This pursuit aligns with broader efforts to enhance patient outcomes, minimize healthcare burdens, and contribute to the global understanding of these prevalent conditions.
Neutrophil depletion for early allogeneic islet survival in a methacrylic acid (MAA) copolymer-induced, vascularized subcutaneous space
Authors: Won So-Yoon , Kinney Sean M. , Sefton Michael V.
Published: October 2023

Islet transplantation is a promising treatment for type I diabetes (T1D). Despite the high loss of islets during transplantation, current islet transplant protocols continue to rely on portal vein infusion and intrahepatic engraftment. Because of the risk of portal vein thrombosis and the loss of islets to instant blood mediated inflammatory reaction (IBMIR), other transplantation sites like the subcutaneous space have been pursued for its large transplant volume, accessibility, and amenability for retrieval. To overcome the minimal vasculature of the subcutaneous space, prevascularization approaches or vascularizing biomaterials have been used to subcutaneously deliver islets into diabetic mice to return them to normoglycemia. Previous vascularization methods have relied on a 4 to 6 week prevascularization timeframe. Here we show that a vascularizing MAA-coated silicone tube can generate sufficient vasculature in 2 to 3 weeks to support a therapeutic dose of islets in mice. In order to fully harness the potential of this prevascularized site, we characterize the unique, subcutaneous immune response to allogeneic islets in the first 7 days following transplantation, a critical stage in successful engraftment. We identify neutrophils as a specific cellular target, a previously overlooked cell in the context of subcutaneous allogeneic islet transplantation. By perioperatively depleting neutrophils, we show that neutrophils are a key, innate immune cell target for successful early engraftment of allogeneic islets in a prevascularized subcutaneous site.
A New Approach to Study the Effect of Complexity on an External Gear Pump Model to Generate Data Source for AI-Based Condition Monitoring Application
Authors: Azeez Abid Abdul , Mazzei Pietro , Minav Tatiana , Frosina Emma , Senatore Adolfo
Published: October 2023

The external gear pump, like any other hydraulic component, is vulnerable to failure, which may lead to downtime as well as the failure of other components linked to it, thereby causing production loss. Therefore, establishing a condition monitoring system is crucial in identifying failure at an early stage. Traditional condition monitoring approaches rely on experimental data that are collected by means of sensors. However, the sensors utilized in the experiments may have calibration issues, which lead to inaccurate measurements. The availability of experimental data is also limited as it is difficult and expensive to create and detect a fault in a component. Hence, it is essential to develop a simulation model that mimics the performance of the actual system. The data generated from the model can be utilized to create the data source required for automated condition monitoring. A new methodology based on a detailed geometric model for simulating the External Gear Pump is described and compared to two models analyzed in the authors’ previous work, namely Schlosser’s loss model and simple geometric model. In this paper, the three models are compared with experimental data and the method utilized for fault injection. Schlosser’s loss model, as well as the detailed geometric model, are found to be suitable in terms of validation; however, the latter is a better candidate in terms of fault injection. Hence, the detailed geometric model can be implemented as a tool to generate the data source for condition monitoring applications.
Extended State Observer-Based Sliding Mode Control Design of Two-DOF Lower Limb Exoskeleton
Authors: Zhang Jiyu , Gao Wei , Guo Qing
Published: October 2023

Due to some model uncertainties and unknown friction disturbances that exist in the 2-DOF lower limb exoskeleton, a linear extended state observer (LESO) is proposed to estimate the unmeasurable angular velocity of two joints and the lumped uncertainties caused by friction disturbance and hydraulic parametric uncertainties. Meanwhile, by using the Lyapunov technique, a sliding mode controller is designed to improve the dynamic performance and the steady state accuracy of two joint angle responses in human–exoskeleton cooperative motion. By regulating the sliding mode controller gain, both the system state errors and estimation errors of the LESO are reduced in an arbitrary boundary of zero neighborhood. Finally, the effectiveness of the proposed control scheme is verified with both simulation and experimental results for one operator-wearable test, to guarantee that the joint position tracking performance and human–exoskeleton impedance torques are suppressed in a satisfactory boundary.
Easily Pyrolyzable Biomass Components Significantly Affect the Physicochemical Properties and Water-Holding Capacity of the Pyrolyzed Biochar
Authors: Zhang Kaizhao , Zhang Kun , Li Yaoming , Kang Qilin , Wang Yaofeng , Wang Jing , Yang Kai , Mao Jiefei
Published: October 2023

The influences of feedstocks on biochar properties are widely reported. However, the influence of the transformation of biomass components (mainly cellulose, hemicellulose, and lignin) during feedstock pyrolysis on the obtained biochar has not been clearly stated. Here, biochar was pyrolyzed from four biomass types with different fractions of the three main components, of which surface area, pore structure, functional group, and thermogravimetric analyses were conducted. Further, we investigated the links among the physicochemical properties and water-holding capacity (WHC) of the biochar by measuring the WHC of a biochar–silica-sand (SS) mixture. Cellulose and hemicellulose were considered the easily pyrolyzable components of the feedstock owing to their low thermal stabilities. Additionally, the thermal decomposition of the easily pyrolyzable components caused the disappearance of most functional groups from the biochar that was synthesized at >350 °C. Moreover, the WHC of the biochar–SS mixture correlated significantly with the surface area and pore volumes of the biochar. Notably, the thermal residual mass and the WHC of the biochar–SS mixture exhibited the strongest correlation. Poplar wood sawdust (PT), which accounted for the highest mesopore volume of the biochar sample, contained the highest amount (86.09%) of the easily pyrolyzable components. The PT-derived biochar exhibited superior WHC than other biochar types, indicating that the dehydration, deoxygenation, and condensation of the easily pyrolyzable components of biomasses promoted gradual pore formation, further contributing to the increased WHC of the mixture. Rather than high-temperature-pyrolyzed biochar, PT350 demonstrated the highest WHC (599 mg/g), revealing that attention should be drawn to the contribution of low-temperature-pyrolyzed biochar to soil water retention in future research.
Effect of Different Levels of Calcium and Addition of Magnesium in the Diet on Garden Snails’ (Cornu aspersum) Condition, Production, and Nutritional Parameters
Authors: Rygało-Galewska Anna , Zglińska Klara , Roguski Mateusz , Roman Kamil , Bendowski Wiktor , Bień Damian , Niemiec Tomasz
Published: October 2023

Edible snails are an attractive protein source due to their high growth rate, cost-efficiency, and nutritional value. Calcium is crucial for snail growth, reproduction, and shell formation, while magnesium plays a role in enzyme function and muscle tone. This study aimed to optimise calcium and magnesium levels in Cornu aspersum diets to optimise the production and technological characteristics of the derived animal products. Snails were fed specific diets in controlled conditions with varying calcium and magnesium levels (44.3, 66.1, 88.7, 103.5 Ca g/kg feed and 3.3, 5.6, 7.2 Mg g/kg feed) for four months. Their growth, shell characteristics, and meat composition were evaluated. As calcium in the feed increased, carcass and shell weights were higher. Also, the crushing force of the shells was higher with increasing amount of calcium in the feed. In the group with 10.35% calcium and 0.72% magnesium, snail growth significantly slowed down after three months, with lower mortality. It is suggested that a shortened fattening cycle by 3–4 weeks compared to the magnesium-free diet is possible. However, based on meat, shell, mortality, and feed intake analysis, a 0.56% magnesium concentration in the feed seems to give better results, as magnesium content at 0.72% might be toxic to snails. Further investigation is to confirm the possibility of neutralising the negative effects of magnesium in the diet through increasing calcium and phosphorus intake.
Spatial Pattern and Influencing Factors of Agricultural Leading Enterprises in Heilongjiang Province, China
Authors: Wang Tianli , Ma Yanji , Luo Siqi
Published: October 2023

As one of the major new agricultural business entities, agricultural leading enterprises (ALEs) are responsible for ensuring national food security, leading agricultural and rural modernization, and increasing farmers’ employment prospects and incomes. From the perspective of headquarters and branches, this study used a point pattern analysis, the local Moran’s index, the rank-size rule, and the geographical detector to depict the spatial pattern of ALEs in Heilongjiang Province, detect influencing factors, and reveal the spatial layout mechanism. The main conclusions are as follows. (1) ALE headquarters and branches in Heilongjiang Province had different location requirements, and their layout orientation, clustering areas, and influencing factors were different. (2) The headquarters displayed a pronounced urban and agglomeration orientation, while branches exhibited a significant farm dependence and raw material orientation. (3) Both the headquarters and the branches showed a significant trend towards spatial clustering. The headquarters were mainly in the Harbin municipal district and surrounding counties, including Wuchang, Zhaodong, and Beilin, which showed a high–high cluster pattern. The branches were mainly in the Sanjiang Plain. Tongjiang, Fujin, Hulin, Mishan, Raohe, Baoqing, and Suibin showed a high–high cluster pattern, while the Harbin municipal district and Bei’an showed a high–low outlier pattern. (4) The ALEs’ regional connection network in Heilongjiang Province was radially distributed with the Harbin municipal district as the centre. The agricultural reclamation system deeply influenced it. (5) The number of supporting enterprises, number of permanent residents, gross domestic product, railway mileage, number of people with Bachelor’s degrees or above, and distance from the provincial capital were the main influencing factors of the headquarters spatial pattern in Heilongjiang Province. The number of state farms in Heilongjiang Province, the per capita grain yield, highway mileage, and distance from the provincial capital were the main influencing factors of the branch spatial patterns in Heilongjiang Province. The interaction results indicated that the explanatory power of two-factor interaction was stronger than that of a single factor regardless of headquarters or branches, and most interaction types were bilinear enhancements. This study aims to provide a decision-making reference for the long-term development of ALEs in Heilongjiang Province at the present stage and accelerate the development of agricultural industrialization in major grain-producing areas.
Pollen Development and Stainability in Vicia faba L. and Lupinus angustifolius L.
Authors: Skrzypkowski Wiktor , Galek Renata , Adamus Adela , Kiełkowska Agnieszka
Published: October 2023

Commercially, leguminous crops (Fabaceae) are the second most important group of cultivated plants, just after grasses (Poaceae). This study focuses on the analysis of pollen development and stainability in two species belonging to the Fabaceae family: Vicia faba L. and Lupinus angustifolius L. Morphological analysis of the anthers at various stages of flower development allowed us to trace the processes of microsporogenesis and microgametogenesis. Nine different cell staining protocols with diverse mechanisms of action, including acetocarmine, Alexander’s dye, aniline blue in lactophenol, Calcein AM, FDA, MTT, TTC, Lugol’s iodine, and aceto-orcein, were tested for their suitability in assessing the viability of microspores as well as pollen grains in both species. Among the applied dyes, four allowed for the discrimination between viable and nonviable microspores in V. faba, and six dyes allowed for this in L. angustifolius. For mature pollen grains, all dyes enabled differentiation between viable and nonviable cells in both species. The highest viability indications for V. faba microspores were obtained with acetocarmine (94.6%), while for mature pollen, aniline blue in lactophenol, MTT, and aceto-orcein yielded the highest viability indications (90.8–96.3%). In L. angustifolius, the highest percentages of viable microspores (64.9–66.5%) were obtained with the acetocarmine, aniline blue in lactophenol, and TTC dyes. For mature pollen, the highest viability indications (83.4%–92.9%) were obtained with acetocarmine, aniline blue in lactophenol, Lugol’s iodine, and aceto-orcein. The viability of V. faba pollen grains in an in vitro germination test showed that the highest pollen germination (61.3%) was observed on the BK medium (rich in minerals with 10% sucrose). In L. angustifolius, the highest pollen germination was observed on the media containing boric acid and 5% sucrose (70.5%) and on the medium containing 10% sucrose only (74.2%).
Pre-Harvest Application of Strigolactone (GR24) Accelerates Strawberry Ripening and Improves Fruit Quality
Authors: Li Mengyao , Yang Min , Liu Xiaoyang , Hou Guoyan , Jiang Yuyan , She Musha , He Caixia , Peng Yuting , Lin Yuanxiu , Zhang Yunting , Wang Yan , He Wen , Chen Qing , Zhang Yong , Wang Xiaorong , Tang Haoru , Luo Ya
Published: October 2023

GR24, a synthetic strigolactone analogue, plays a crucial role in a wide range of life processes. Studying the effects of the pre-harvest application of GR24 on strawberry ripening and fruit quality provides a scientific basis for the application of GR24 in horticultural crop production. GR24 solutions at concentrations of 0, 0.5, 1 and 2 µmol/L were applied to strawberries at the de-greening stage. The results showed that pre-harvest treatment with 1 µmol/L GR24 increased fruit weight and size; promoted fruit softening; increased the content of sugars, total flavonoids, total phenolics, and anthocyanins, and increased antioxidant activity. These results proved that GR24 effectively accelerated fruit ripening and improved fruit quality. In addition, to gain more insight into the biological mechanism of GR24 in fruit ripening, we conducted a combined transcriptomic and metabolomic analysis. It was found that the differences were related to sugar and flavonoid biosynthesis, particularly in the later stage of fruit ripening. qRT-PCR analysis revealed that GR24 increased gene expression activity and metabolite accumulation in the anthocyanin and sugar biosynthetic pathway, thereby promoting strawberry color and flavor.