bluuebunny/crossref_metadata_2025 huggingface dataset:
Supportive care needs of women with gynaecological cancer: A systematic review and synthesis of qualitative studies
Authors: Sun Yue , Ma Yanling , Li Qiaoran , Ge Jingling
Published: February 2023

Aims

To synthesize existing qualitative research evidence on the supportive care needs of women with gynaecological cancer.

Design

Qualitative systematic review.

Data sources

A comprehensive literature search was performed using nine databases (PubMed, Web of Science, PsycINFO, CINAHL, Embase, CBM, CNKI, VIP and WanFang) without restrictions regarding publication date; qualitative studies published in English or Chinese were included. Initial search in December 2021 and updated in October 2022.

Review methods

This study was conducted according to the Enhancing Transparency in Reporting the Synthesis of Qualitative Research (ENTREQ) guidelines. The Critical Appraisal Skills Programme tool for qualitative research was used to assess the quality of all included papers. Finally, we adopted a thematic synthesis method, synthesized the main findings, and constructed themes.

Results

Eleven studies published between 2010 and 2021 were included in the review. Based on the thematic synthesis method, 10 descriptive themes were generated and five analytical themes were derived: psychological support, information support, social support, disease‐specific symptom management and form of care. Women with gynaecological cancer expressed a desire for psychological support from empathetic healthcare professionals; information support included access to adequate and appropriate information, as well as communication and involvement; social support highlighted women's desire for peer support, family‐related support and financial support; disease‐specific symptom management described women's desire for support in coping with reproduction/sexual issues and form of care highlighted the need for continuity of care and holistic care.

Conclusion

The supportive care needs of women with gynaecological cancer are multidimensional and complex. The future care practice should take women's needs as a starting point and provide ongoing holistic and individualized support. Healthcare providers' understanding and support of these needs are critical to improving women's clinical outcomes and quality of care.

Impact

The present findings can help further develop supportive care programmes and make nursing interventions more targeted and effective.

Patient or Public Contribution

No patient or public contribution.
Costunolide alleviates hyperglycaemia‐induced diabetic cardiomyopathy via inhibiting inflammatory responses and oxidative stress
Authors: Jin Bo , Chen Yi , Wang Jiong , Chen Yue , Zhang Mengpei , Huang Jianxiong , Wang Yi
Published: February 2023

Hyperglycaemia‐induced myocardial injury promotes the induction of heart failure in diabetic patients. Impaired antioxidant capability and sustained chronic inflammation play a vital role in the progression of diabetic cardiomyopathy (DCM). Costunolide (Cos), a natural compound with anti‐inflammatory and antioxidant properties, has exhibited therapeutic effects in various inflammatory diseases. However, the role of Cos in diabetes‐induced myocardial injury remains poorly understood. In this study, we investigated the effect of Cos on DCM and explored the potential mechanisms. C57BL/6 mice were administered intraperitoneal streptozotocin for DCM induction. Cos‐mediated anti‐inflammatory and antioxidation activities were examined in heart tissues of diabetic mice and high glucose (HG)‐stimulated cardiomyocytes. Cos markedly inhibited HG‐induced fibrotic responses in diabetic mice and H9c2 cells, respectively. The cardioprotective effects of Cos could be correlated to the reduced expression of inflammatory cytokines and decreased oxidative stress. Further investigations demonstrated Cos reversed diabetes‐induced nuclear factor‐κB (NF‐κB) activation and alleviated impaired antioxidant defence system, principally via activation of nuclear factor‐erythroid 2 p45‐related factor‐2 (Nrf‐2). Cos alleviated cardiac damage and improved cardiac function in diabetic mice by inhibiting NF‐κB‐mediated inflammatory responses and activating the Nrf‐2‐mediated antioxidant effects. Therefore, Cos could be a potential candidate for the treatment of DCM.
Study of the effects of in‐person attendance at academic conferences on the health of the attendees under COVID‐19 pandemic
Authors: Yahata Hideaki , Kato Kiyoko , Shimokawa Mototsugu , Kawamura Keiko , Shimono Nobuyuki , Kawana Kei , Okamoto Aikou , Aoki Daisuke , Kimura Tadashi
Published: February 2023

Objective

To analyze the effects of in‐person attendance at an academic conference held during the Covid‐19 pandemic on the health of the attendees, as assessed based on symptoms such as fever and cough attributed to infection with the Covid‐19 virus.

Methods

A questionnaire was used to survey the members of the Japan Society of Obstetrics and Gynecology (JSOG) about their health during the period from August 7 to August 12, 2022, after the 74th Annual Congress of the JSOG, which was held August 5 to 7.

Results

Our survey yielded responses from 3054 members (1566 of whom had attended the congress in person and 1488 of whom had not attended in person); 102 (6.5%) of the in‐person attendees and 93 (6.2%) of the people who did not attend in person reported problems with their health. No statistically significant difference was found between these two groups (p = 0.766). In a univariate analysis of factors affecting the presence of health problems, attendees with age ≥60 years had significantly fewer health problems than attendees who were in their 20s (odds ratio: 0.366 [0.167–0.802; p = 0.0120]). In a multivariate analysis, attendees who had received four vaccine shots had significantly fewer health problems than attendees who had received three shots (odds ratio: 0.397 [0.229–0.690, p = 0.0010]).

Conclusion

Congress attendees who took precautions at the congress to avoid being infected and who had a high vaccination rate did not develop significantly more health problems associated with in‐person attendance at the congress.
Effect of a new support design on the marginal and internal gap of additively manufactured interim crowns using direct light deposition technology
Authors: Khanlar Leila Nasiry , Francis Claire , Basir Barmak Abdul , Rios Alma L. Salazar , Ashtiani Reza Eftekhar , Zandinejad Amirali
Published: February 2023

Purpose

To investigate the design and location of supporting structures on the marginal and internal gap of interim restorations.

Materials and methods

A mandibular right first molar resin tooth was prepared for a full coverage crown and scanned using a laboratory scanner (3Shape D900). The scanned data were converted into standard tessellation language (STL) format and an indirect prosthesis was designed using computer‐aided design (CAD) software (exocad DentalCAD). The STL file was used to fabricate a total of 60 crowns with a 3D printer (EnvisionTEC Vida HD). The crowns were printed using E‐Dent C&B MH resin and divided into 4 groups based on four different support structure designs, including supports on the occlusal (0° group), buccal and occlusal (45° group), buccal (90° group), and a new design consisting of horizontal bars placed on all surfaces and line angles (Bar) (n = 15). The silicone replica technique was used to determine the gap discrepancy. Fifty measurements were obtained for each specimen to examine the marginal and internal gaps by using a digital microscope (Olympus SZX16) at ×70 magnification. Additionally, the marginal discrepancy at different locations of the tested crowns, including buccal (B), lingual (L), mesial (M), and distal (D), as well as the maximum and minimum marginal gap intervals among groups, were analyzed. The collected data were analyzed using factorial ANOVA, followed by the Tukey HSD test for multiple comparisons (a = 0.05).

Results

There was a significant difference in marginal and internal gaps among the groups (p < 0.001). The buccal placement supports (90° group) had the least marginal and internal discrepancies (p < 0.001). The new design group showed the highest marginal and internal gap. The marginal discrepancy in different locations of the tested crowns (B, L, M, D) was found to be significantly different among the groups (p < 0.001). The mesial margin of the Bar group had the largest marginal gap, whereas the buccal margin of the 90° group had the lowest marginal gap. The new design had a significantly smaller difference between the maximum and minimum marginal gap intervals than other groups (p < 0.001).

Conclusion

The location and design of the supporting structures affected the marginal and internal gaps of an interim crown. The buccal placement of supporting bars (90° printing orientation) showed the smallest mean internal and marginal discrepancies.
Rapid introgression of non‐native alleles following hybridization between a native Anolis lizard species and a cryptic invader across an urban landscape
Authors: DeVos Tyler B. , Bock Dan G. , Kolbe Jason J.
Published: February 2023

Invasive species can impact native populations through competition, predation, habitat alteration, and disease transmission, but also genetically through hybridization. Potential outcomes of hybridization span the continuum from extinction to hybrid speciation and can be further complicated by anthropogenic habitat disturbance. Hybridization between the native green anole lizard (Anolis carolinensis) and a morphologically similar invader (A. porcatus) in south Florida provides an ideal opportunity to study interspecific admixture across a heterogeneous landscape. We used reduced‐representation sequencing to describe introgression in this hybrid system and to test for a relationship between urbanization and non‐native ancestry. Our findings indicate that hybridization between green anole lineages was probably a limited, historic event, producing a hybrid population characterized by a diverse continuum of ancestry proportions. Genomic cline analyses revealed rapid introgression and disproportionate representation of non‐native alleles at many loci and no evidence for reproductive isolation between parental species. Three loci were associated with urban habitat characteristics; urbanization and non‐native ancestry were positively correlated, although this relationship did not remain significant when accounting for spatial nonindependence. Ultimately, our study demonstrates the persistence of non‐native genetic material even in the absence of ongoing immigration, indicating that selection favouring non‐native alleles can override the demographic limitation of low propagule pressure. We also note that not all outcomes of admixture between native and non‐native species should be considered intrinsically negative. Hybridization with ecologically robust invaders can lead to adaptive introgression, which may facilitate the long‐term survival of native populations otherwise unable to adapt to anthropogenically mediated global change.
Peptide signaling through leucine‐rich repeat receptor kinases: insight into land plant evolution
Authors: Furumizu Chihiro , Aalen Reidunn Birgitta
Published: February 2023

Multicellular organisms need mechanisms for communication between cells so that they can fulfill their purpose in the organism as a whole. Over the last two decades, several small post‐translationally modified peptides (PTMPs) have been identified as components of cell‐to‐cell signaling modules in flowering plants. Such peptides most often influence growth and development of organs not universally conserved among land plants. PTMPs have been matched to subfamily XI leucine‐rich repeat receptor‐like kinases with > 20 repeats. Phylogenetic analyses, facilitated by recently published genomic sequences of non‐flowering plants, have identified seven clades of such receptors with a history back to the common ancestor of bryophytes and vascular plants. This raises a number of questions: When did peptide signaling arise during land plant evolution? Have orthologous peptide–receptor pairs preserved their biological functions? Has peptide signaling contributed to major innovations, such as stomata, vasculature, roots, seeds, and flowers? Using genomic, genetic, biochemical, and structural data and non‐angiosperm model species, it is now possible to address these questions. The vast number of peptides that have not yet found their partners suggests furthermore that we have far more to learn about peptide signaling in the coming decades.
Automatic landmark identification in cone‐beam computed tomography
Authors: Gillot Maxime , Miranda Felicia , Baquero Baptiste , Ruellas Antonio , Gurgel Marcela , Al Turkestani Najla , Anchling Luc , Hutin Nathan , Biggs Elizabeth , Yatabe Marilia , Paniagua Beatriz , Fillion‐Robin Jean‐Christophe , Allemang David , Bianchi Jonas , Cevidanes Lucia , Prieto Juan Carlos
Published: February 2023

Objective

To present and validate an open‐source fully automated landmark placement (ALICBCT) tool for cone‐beam computed tomography scans.

Materials and Methods

One hundred and forty‐three large and medium field of view cone‐beam computed tomography (CBCT) were used to train and test a novel approach, called ALICBCT that reformulates landmark detection as a classification problem through a virtual agent placed inside volumetric images. The landmark agents were trained to navigate in a multi‐scale volumetric space to reach the estimated landmark position. The agent movements decision relies on a combination of DenseNet feature network and fully connected layers. For each CBCT, 32 ground truth landmark positions were identified by 2 clinician experts. After validation of the 32 landmarks, new models were trained to identify a total of 119 landmarks that are commonly used in clinical studies for the quantification of changes in bone morphology and tooth position.

Results

Our method achieved a high accuracy with an average of 1.54 ± 0.87 mm error for the 32 landmark positions with rare failures, taking an average of 4.2 second computation time to identify each landmark in one large 3D‐CBCT scan using a conventional GPU.

Conclusion

The ALICBCT algorithm is a robust automatic identification tool that has been deployed for clinical and research use as an extension in the 3D Slicer platform allowing continuous updates for increased precision.
Wider indications and more efficient drug delivery: A bibliometric analysis and visualization of photodynamic therapy in skin cancer
Authors: Jiang Zixi , Xu Yantao , Zhu Zirui , Liu Yuancheng , Zhang Liang , Jian Dan
Published: February 2023

Background

Photodynamic therapy (PDT) has been widely accepted in keratocyte carcinoma and an increasing number of literatures concerning PDT in skin cancer is published. But a detailed examination of publication patterns of PDT in skin cancer has not yet been carried out.

Methods

Bibliographies were retrieved from Web of Science Core Collection restricted the publication date from January 1, 1985 to December 31, 2021. The search terms were photodynamic therapy and skin cancer. Visualization analysis and statistical analysis were performed by VOSviewer (Version 1.6.13), R software (Version 4.1.2) and Scimago Graphica (Version 1.0.15).

Results

3248 documents were selected for analysis. The results showed that the number of annual publications related to PDT in skin cancer was gradually increased and would continue to increase in the future. The results illustrated that “melanoma”, “nanoparticles”, “drug‐delivery”, “mechanism”, “delivery” and “in‐vitro” are newly occurred topics. The most prolific country was the United States and the most productive institution was the University of Sao Paulo in Brazil. German researcher Szeimies RM published the most papers related to PDT in skin cancer. British Journal of Dermatology was the most popular journal in this field.

Conclusion

The topic that PDT in skin cancer is a heated issue. Our study revealed the bibliometric result of the field, which might provide the prospects for further research. We recommend future investigations focusing on PDT in treating melanoma, innovation of photosensitizer, improvement of drug delivery and the mechanism of PDT in skin cancer.
Effects of communicating lifetime risks and screening rates of colorectal cancer and breast cancer
Authors: Liu Jiawei , Niederdeppe Jeff
Published: February 2023

Colorectal cancer and breast cancer are among the most common types of cancer in the United States, and cancer screening is an effective way to detect and treat these cancers early. Health news stories, medical websites, and media campaigns regularly highlight the national lifetime risks of specific cancers and their screening rates, but recent research suggests that people tend to overestimate the prevalence of health problems but underestimate the prevalence of disease prevention behaviors in the absence of numerical information. This study featured two online experiments, one focused on breast cancer (N = 632) and one focused on colorectal cancer (N = 671), to examine the effects of communicating national cancer lifetime risks and screening rates among samples of screening‐eligible adults in the United States. Findings confirmed prior work in showing that people overestimated colorectal/breast cancer lifetime risks but underestimated colorectal/breast cancer screening rates. Communicating the national lifetime risk of dying from colorectal/breast cancer lowered people's national risk estimates, which in turn was associated with lower perceived cancer risks for themselves. In contrast, communicating the national colorectal/breast cancer screening rate increased people's estimates of the prevalence of cancer screening, which in turn was associated with higher perceived self‐efficacy to engage in cancer screening and greater screening intentions. We conclude that efforts to promote cancer screening may benefit from messages that include data on national cancer screening rates but may not benefit from including national rates of lifetime cancer risks.
Identification of the novel HLA‐C allele HLA‐C*12:02:02:22, in two individuals from Central India
Authors: Neeraja Mamidi , Iyyapu Rohit , Raj Kumar Neerudi , GorleManoj  , Hussain Shaik Farooq
Published: February 2023

One nucleotide change (G > C) in intron 1 of HLA‐C*12:02:02:01 results in novel HLA allele HLA‐C*12:02:02:22.
An optimized transformation protocol for Anthoceros agrestis and three more hornwort species
Authors: Waller Manuel , Frangedakis Eftychios , Marron Alan O. , Sauret‐Güeto Susanna , Rever Jenna , Sabbagh Cyrus Raja Rubenstein , Hibberd Julian M. , Haseloff Jim , Renzaglia Karen S. , Szövényi Péter
Published: February 2023

Land plants comprise two large monophyletic lineages, the vascular plants and the bryophytes, which diverged from their most recent common ancestor approximately 480 million years ago. Of the three lineages of bryophytes, only the mosses and the liverworts are systematically investigated, while the hornworts are understudied. Despite their importance for understanding fundamental questions of land plant evolution, they only recently became amenable to experimental investigation, with Anthoceros agrestis being developed as a hornwort model system. Availability of a high‐quality genome assembly and a recently developed genetic transformation technique makes A. agrestis an attractive model species for hornworts. Here we describe an updated and optimized transformation protocol for A. agrestis, which can be successfully used to genetically modify one more strain of A. agrestis and three more hornwort species, Anthoceros punctatus, Leiosporoceros dussii, and Phaeoceros carolinianus. The new transformation method is less laborious, faster, and results in the generation of greatly increased numbers of transformants compared with the previous method. We have also developed a new selection marker for transformation. Finally, we report the development of a set of different cellular localization signal peptides for hornworts providing new tools to better understand the hornwort cell biology.
Comparison Between Three Methods to Monitor Reservoir Extension in the Brazilian Semi-Arid Region
Authors: Dos Anjos Carvalho Wilson , Cezar Bezerra Alan , Alba Elisiane , Sandra bastos Souza Luciana , Santos da Silva Anderson , Barbosa de Albuquerque Moura Geber
Published: February 2023

The State of Pernambuco covers an extensive semi-arid area where the Caatinga biome dominates. This region is characterized by long periods of drought, highlighting the need for water resource optimization. This paper aimed to compare three methods to assess reservoir changes: MapBiomas' products, the Normalized Difference Water Index (NDWI), and a support vector machine (SVM) algorithm. Initially, we obtained the monthly precipitation from 1987 to 2019 and calculated the yearly accumulation. Mapbiomas, Landsat 7 ETM, and Landsat 8 OLI data from 2012-2018 were accessed and processed using the Google Earth Engine platform. We obtained the annual image with the median pixel criterion to determine the NDWI and quantify the annual reservoir area. For the supervised classification with SVM, samples from different land-use types of the study area were used to train the algorithm. From 2012 to 2018, a reservoir reduction of 63.42% was observed with MapBiomas images, 69.49% with NDWI images, and 67.69% using the SVM algorithm. The results obtained using NDWI were the most similar to those from the artificial intelligence classification, indicating that NDWI can be used to monitor the reservoir conditions.
Reversible alteration of 3D printed polymer properties via infiltration of alumina by atomic layer deposition
Authors: Varga Atilla C. , Barry Seán T.
Published: February 2023

3D printed acrylonitrile butadiene styrene (ABS) and polyvinyl alcohol (PVA) structures were infiltrated by alumina (Al2O3) using a trimethylaluminum(III) and water ALD process at 130 and 80 °C, respectively, to alter their physical properties. Differential scanning calorimetry was used to determine the glass transition temperature (Tg) of the polymers' pre- and post-deposition after varying the number of ALD cycles, resulting in a change of ∼9 and ∼ 27 °C for ABS and PVA, respectively. After one heat cycle, the postdeposition Tg reverted back to its predisposition point indicating reversibility of the deposition effects are possible. Optimal growing patterns, polymer composition, and inhibiting surface coatings—seen by energy-dispersive x-ray spectroscopy mapping—affected the amount of infiltration possible within the polymer substrate and, in turn, Tg. The results achieved provide guidelines to altering the physical and thermal properties of 3D printed polymer architectures.
An efficient sparse method for direction-of-arrival estimation in the presence of strong interference
Authors: Li Chenmu , Liang Guolong , Qiu Longhao , Shen Tongsheng , Zhao Lei
Published: February 2023

Target direction-of-arrival (DOA) estimation is often difficult in the presence of strong interference—especially when the target DOA is very close to the interference DOA—since the strong interference signal can mask the weak target signal and make the DOA estimation hard. To address this problem, an efficient sparse method for DOA estimation is proposed in this paper, in which the effect of strong interference on the target DOA estimation is significantly reduced. An on-grid version of the grid evolution technique is then developed to nonuniformly refine the grid, thereby reducing the computational complexity while retaining reasonable accuracy. Numerical simulations and experimental results demonstrate that, compared to state-of-art methods, the proposed method achieves higher efficiency and better DOA estimation performance in the presence of strong interference.
Single-Leg Hop Stabilization Throughout Concussion Recovery: A Preliminary Biomechanical Assessment
Authors: Lempke Landon B. , Hoch Matthew C. , Call Jarrod A. , Schmidt Julianne D. , Lynall Robert C.
Published: February 2023

Context: Aberrant movement patterns among individuals with concussion history have been reported during sport-related movement. However, the acute postconcussion kinematic and kinetic biomechanical movement patterns during a rapid acceleration–deceleration task have not been profiled and leaves their progressive trajectory unknown. Our study aimed to examine single-leg hop stabilization kinematics and kinetics between concussed and healthy-matched controls acutely (≤7 d) and when asymptomatic (≤72 h of symptom resolution). Design: Prospective, cohort laboratory study. Methods: Ten concussed (60% male; 19.2 [0.9] y; 178.7 [14.0] cm; 71.3 [18.0] kg) and 10 matched controls (60% male; 19.5 [1.2] y; 176.1 [12.6] cm; 71.0 [17.0] kg) completed the single-leg hop stabilization task under single and dual task (subtracting by 6’s or 7’s) at both time points. Participants stood on a 30-cm tall box set 50% of their height behind force plates while in an athletic stance. A synchronized light was illuminated randomly, queuing participants to initiate the movement as rapidly as possible. Participants then jumped forward, landed on their nondominant leg, and were instructed to reach and maintain stabilization as fast as possible upon ground contact. We used 2 (group) × 2 (time) mixed-model analyses of variance to compare single-leg hop stabilization outcomes separately during single and dual task. Results: We observed a significant main group effect for single-task ankle plantarflexion moment, with greater normalized torque (mean difference = 0.03 N·m/body weight; P = .048, g = 1.18) for concussed individuals across time points. A significant interaction effect for single-task reaction time indicated that concussed individuals had slower performance acutely relative to asymptomatic (mean difference = 0.09 s; P = .015, g = 0.64), while control group performance was stable. No other main or interaction effects for single-leg hop stabilization task metrics were present during single and dual task (P ≥ .051). Conclusions: Greater ankle plantarflexion torque coupled with slower reaction time may indicate stiff, conservative single-leg hop stabilization performance acutely following concussion. Our findings shed preliminary light on the recovery trajectories of biomechanical alterations following concussion and provide specific kinematic and kinetic focal points for future research.
Investigating Variability in Microbial Fuel Cells
Authors: Leicester Daniel David , Settle Sam , McCann Clare M. , Heidrich Elizabeth Susan
Published: February 2023

The research presented, although carried out in the area of microbial fuel cells, reaches an important and broadly impacting conclusion that when using mixed inoculate in replica reactors under replicated conditions, different communities emerge capable of different levels of metabolism. To date there has been very little research focusing on this, or even reporting it, with most studies using duplicate or triplicate reactors, in which this phenomenon is not fully observed.
Machine-Learning Model for Prediction of Cefepime Susceptibility in Escherichia coli from Whole-Genome Sequencing Data
Authors: Humphries Romney M. , Bragin Eugene , Parkhill Julian , Morales Grace , Schmitz Jonathan E. , Rhodes Paul A.
Published: February 2023

The declining cost of performing bacterial whole-genome sequencing (WGS) coupled with the availability of large libraries of sequence data for well-characterized isolates have enabled the application of machine-learning (ML) methods to the development of nonlinear sequence-based predictive models. We tested the ML-based model developed by Next Gen Diagnostics for prediction of cefepime phenotypic susceptibility results in
Escherichia coli
.
Undergraduate STEM Students’ Science Communication Skills, Science Identity, and Science Self-Efficacy Influence Their Motivations and Behaviors in STEM Community Engagement
Authors: Murphy Katlyn M. , Kelp Nicole C.
Published: February 2023

While numerous studies have examined how scientists perceive doing public communication and engagement, there is limited research on undergraduate science, technology, engineering, and math (STEM) student attitudes toward these meaningful activities. Undergraduate students are more diverse than STEM faculty and may serve as boundary spanners in communities, so exploring their motivations and behaviors in STEM engagement is valuable.
Key Residue in the Precursor Region of M Protein Contributes to the Neurovirulence and Neuroinvasiveness of the African Lineage of Zika Virus
Authors: He Meng-Jiao , Wang Hong-Jiang , Yan Xiu-Li , Lou Ya-Nan , Song Guang-Yuan , Li Rui-Ting , Zhu Zhu , Zhang Rong-Rong , Qin Cheng-Feng , Li Xiao-Feng
Published: February 2023

The suspected link of Zika virus (ZIKV) to birth defects led the World Health Organization to declare ZIKV a Public Health Emergency of International Concern. ZIKV has been identified to have two dominant phylogenetic lineages, African and Asian.
Draft Genome Sequence of
Thermomicrobium
sp. Strain 4228-Ro, a Thermophilic Bacterium Isolated from a Kamchatka Hot Spring
Authors: Podosokorskaya Olga A. , Klyukina Alexandra A. , Panova Tatiana V. , Rodin Vladimir A. , Zvereva Maria I. , Tulenkov Adolf S. , Lebedinsky Alexander V. , Kublanov Ilya V. , Elcheninov Alexander G.
Published: February 2023

The genome of
Thermomicrobium
sp. strain 4228-Ro, an aerobic thermophilic bacterium isolated from a Kamchatka hot spring, was sequenced and analyzed. The genome assembly comprises 13 contigs with a total length of 3,068,448 bp. Genome analysis revealed the pathway of aerobic utilization of sugars, which was corroborated by growth experiments.
Draft Genome Sequence of Lactococcus lactis Strain 12-16-PSH, Isolated from Prostokvasha from the Arkhangelsk Region of Russia
Authors: Elcheninov Alexander G. , Zayulina Kseniya S. , Shcherbakova Polina A. , Kremneva Mariia K. , Gavirova Liliya A. , Shestakov Andrey I. , Kublanov Ilya V.
Published: February 2023

A draft genome sequence of
Lactococcus lactis
strain 12-16-PSH, which was isolated from prostokvasha, is reported. The genome assembly of strain 12-16-PSH contained 63 contigs, with a total length of 2,468,647 bp. A total of 2,421 protein-coding genes were predicted, among which 6 encoded bacteriocins while 15 encoded glycosyl transferases, presumably involved in exopolysaccharide biosynthesis.
16S rRNA Gene Amplicon Sequencing Data for Pteris vittata Rhizosphere Soils
Authors: Mu’azu Aminu Salisu , Haris Hazzeman , Zarkasi Kamarul Zaman , Lau Nyok-Sean , Ghazali Amir Hamzah
Published: February 2023

Data on the 16S rRNA gene amplicon sequences from
Pteris vittata
rhizosphere soils are reported. The following phyla were recorded in arsenic-rich soils:
Actinobacteria
(59%),
Proteobacteria
(26%),
Chloroflexi
(17%), and
Acidobacteria
(9%).
Actinobacteria
(45%),
Proteobacteria
(22%),
Chloroflexi
(10%), and
Acidobacteria
(11%) were in natural-mineral soils.
Draft Genome Sequences of Three Bacterial Species from Aquatic Habitats near Washington, DC
Authors: Leff Riley T. , MacDougall Jonathan , Pavloudi Christina , Oliver Lausanne , Slattery Kaitlynn , Lissner Guinevere , Saw Jimmy H.
Published: February 2023

We report the draft genome sequences of three bacterial species isolated from freshwater ponds or features around monuments in Washington, DC, during a semester-long microbiology lab course at the George Washington University. Two of the isolates belong to potentially novel species but lost their viability and could not be revived.
Evaluation of an Engineered Zika Virus-Like Particle Vaccine Candidate in a Mosquito-Mouse Transmission Model
Authors: Mancini Maria Vittoria , Tandavanitj Rapeepat , Ant Thomas H. , Murdochy Shivan M. , Gingell Daniel D. , Setthapramote Chayanee , Natsrita Piyatida , Kohl Alain , Sinkins Steven P. , Patel Arvind H. , De Lorenzo Giuditta
Published: February 2023

Zika is a mosquito-borne viral disease, causing acute debilitating symptoms and complications in infected individuals and irreversible neuronal abnormalities in newborn children. The primary vectors of ZIKV are
Aedes aegypti
mosquitoes.
A Cross-Sectional Study of Companion Animal-Derived Multidrug-Resistant Escherichia coli in Hangzhou, China
Authors: Teng Lin , Feng Mengyao , Liao Sihao , Zheng Zhijie , Jia Chenghao , Zhou Xin , Nambiar Reshma B. , Ma Zhengxin , Yue Min
Published: February 2023

MDR
Escherichia coli
are considered a global threat because of the decreasing options for antimicrobial therapy. Companion animals could be a reservoir of MDR
E. coli
, and the numbers of pets and households owning pets in China are booming.
Genomic Characteristics and Molecular Epidemiology of Multidrug-Resistant Klebsiella pneumoniae Strains Carried by Wild Birds
Authors: Wang Xue , Zhao Jianan , Ji Fang , Wang Meng , Wu Bin , Qin Jianhua , Dong Guoying , Zhao Ruili , Wang Chengmin
Published: February 2023

Little is known about the pathogenic microorganisms carried by wild animals. This study found that the multidrug resistance phenotype of
Klebsiella pneumoniae
isolates carried by wild birds was mainly attributed to multidrug resistance plasmids, and these multidrug resistance plasmids from wild birds were closely related to human-infecting bacteria.
Fusobacterium nucleatum Infection Induces Malignant Proliferation of Esophageal Squamous Cell Carcinoma Cell by Putrescine Production
Authors: Ding Ning , Cheng Yikun , Liu Huijuan , Wu Yueguang , Weng Yongjia , Cui Heyang , Cheng Chen , Zhang Weimin , Cui Yongping
Published: February 2023

Nowadays, the complex and varied interactions between microbes and human body are known to be crucial for maintaining the health of the human body. However, knowledge concerning the influence of esophageal microbes on the progression of esophageal squamous cell carcinoma is limited.
Recognition of tRNA
His
in an RNase P-Free Nanoarchaeum
Authors: Ivanesthi Indira Rizqita , Rida Gita Riswana Nawung , Setiawibawa Aditya Aryandi , Tseng Yi-Kuan , Muammar Arief , Wang Chien-Chia
Published: February 2023

Mature tRNA
His
has, at its 5′-terminus, an extra guanosine with 5′-monophosphate, designated G-1. G-1 is the major recognition element for histidyl-tRNA synthetase (HisRS), regardless of whether it is of eukaryotic or prokaryotic origin.
Intermediary roles of prospective memory and retrospective memory in the comorbidity of depression and pain
Authors: Gao Hua , Xia Qingrong , Zhang Xulai , Chen Yuanyuan , Yan Junwei , Gao Jianliang , Zhang Loufeng , Zhang Jie , Wang Keming , Li Chao , Ju Peijun , Zhu Cuizhen
Published: February 2023

Background

Patients who suffer comorbidity of major depressive disorder (MDD) and chronic pain (CP) maintain a complex interplay between maladaptive prospective memory (PM) and retrospective memory (RM) with physical pain, and their complications are still unknown.

Aims

We aimed to focus on the full cognitive performance and memory complaints in patients with MDD and CP, patients with depression without CP, and control subjects, considering the possible influence of depressed affect and chronic pain severity.

Methods

According to the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders and the criteria given by the International Association of Pain, a total of 124 participants were included in this cross-sectional cohort study. Among them, 82 depressed inpatients and outpatients from Anhui Mental Health centre were divided into two groups: a comorbidity group(patients with MDD and CP) (n=40) and a depression group (patients with depression without CP) (n=42). Meanwhile, 42 healthy controls were screened from the hospital’s physical examination centre from January 2019 to January 2022. The Hamilton Depression Rating Scale-24 (HAMD-24) and Beck Depression Inventory-II (BDI-II) were used to evaluate the severity of depression. The Pain Intensity Numerical Rating Scale (PI-NRS), Short-Form McGill Pain Questionnaire-2 Chinese version (SF-MPQ-2-CN), Montreal Cognitive Assessment-Basic Section (MoCA-BC), and Prospective and Retrospective Memory Questionnaire (PRMQ) were used to assess pain-related features and the global cognitive functioning of study participants.

Results

The impairments in PM and RM differed remarkably among the three groups (F=7.221, p<0.001; F=7.408, p<0.001) and were severe in the comorbidity group. Spearman correlation analysis revealed the PM and RM were positively correlated with continuous pain and neuropathic pain (r=0.431, p<0.001; r=0.253, p=0.022 and r=0.415, p<0.001; r=0.247, p=0.025), respectively. Regression analysis indicated a significant positive relationship between affective descriptors and total BDI-II score (β=0.594, t=6.600, p<0.001). Examining the mediator pathways revealed the indirect role of PM and RM in patients with comorbid MDD and CP.

Conclusions

Patients with comorbid MDD and CP presented more PM and RM impairments than patients with MDD without CP. PM and RM are possibly mediating factors that affect the aetiology of comorbid MDD and CP.

Trial registration number

chiCTR2000029917.
Expression, assessment and significance of Ki67 expression in breast cancer: an update
Authors: Lashen Ayat Gamal , Toss Michael S , Ghannam Suzan Fathy , Makhlouf Shorouk , Green Andrew , Mongan Nigel P , Rakha Emad
Published: February 2023

Ki67 expression is one of the most important and cost-effective surrogate markers to assess for tumour cell proliferation in breast cancer (BC). The Ki67 labelling index has prognostic and predictive value in patients with early-stage BC, particularly in the hormone receptor-positive, HER2 (human epidermal growth factor receptor 2)-negative (luminal) tumours. However, many challenges exist in using Ki67 in routine clinical practice and it is still not universally used in the clinical setting. Addressing these challenges can potentially improve the clinical utility of Ki67 in BC. In this article, we review the function, immunohistochemical (IHC) expression, methods for scoring and interpretation of results as well as address several challenges of Ki67 assessment in BC. The prodigious attention associated with use of Ki67 IHC as a prognostic marker in BC resulted in high expectation and overestimation of its performance. However, the realisation of some pitfalls and disadvantages, which are expected with any similar markers, resulted in an increasing criticism of its clinical use. It is time to consider a pragmatic approach and weigh the benefits against the weaknesses and identify factors to achieve the best clinical utility. Here we highlight the strengths of its performance and provide some insights to overcome the existing challenges.
Justification of principles for healthcare priority setting: the relevance and roles of empirical studies exploring public values
Authors: Gustavsson Erik , Lindblom Lars
Published: February 2023

How should scarce healthcare resources be distributed? This is a contentious issue that became especially pressing during the pandemic. It is often emphasised that studies exploring public views about this question provide valuable input to the issue of healthcare priority setting. While there has been a vast number of such studies it is rarely articulated, more specifically, what the results from these studies would mean for the justification of principles for priority setting. On the one hand, it seems unreasonable that public values would straightforwardly decide the ethical question of how resources should be distributed. On the other hand, in a democratic society, it seems equally unreasonable that they would be considered irrelevant for this question. In this paper we draw on the notion of reflective equilibrium and discuss the relevance and roles that empirical studies may plausibly have for justification in priority setting ethics. We develop a framework for analysing how different kinds of empirical results may have different kinds of implications for justification.
Creatine supplementation combined with blood flow restriction training enhances muscle thickness and performance: a randomized, placebo-controlled, and double-blind study
Authors: Sousa-Silva Rayssa , Cholewa Jason M. , Pessôa Kassiana de Araújo , Xia Zhi , Lauver Jakob D. , Rossi Fabrício E. , Zanchi Nelo Eidy
Published: February 2023

This study aimed to compare the effects of an 8-week creatine (CR) or placebo (PL) supplementation on muscle strength, thickness, endurance, and body composition employing different training paradigms with blood flow restriction (BFR) vs. traditional resistance training (TRAD). Seventeen healthy males were randomized between the PL ( n = 9) and CR ( n = 8) groups. Participants were trained unilaterally utilizing a within-between subject bicep curl exercise where each arm was allocated to TRAD or BFR for 8 weeks. Muscular strength, thickness, endurance, and body composition were evaluated. Creatine supplementation promoted increases in muscle thickness in TRAD and BFR compared with their placebo counterparts, however, without a significant difference between treatments ( p = 0.349). TRAD training increased maximum strength (1 repetition maximum (1RM)) compared with BFR after 8 weeks of training ( p = 0.021). Repetitions to failure at 30% of 1RM were increased in the BFR-CR group compared with the TRAD-CR group ( p = 0.004). Repetitions to failure at 70% 1RM were increased from weeks 0–4 ( p < 0.05) and 4–8 ( p < 0.05) in all groups. Creatine supplementation exerted a hypertrophic effect when utilized with TRAD and BFR paradigms and increased muscle performance at 30% 1RM when utilized in conjunction with BFR. Therefore, creatine supplementation seems to amplify muscle adaptation following a BFR program. Registered in the Brazilian Registry of Clinical Trials (ReBEC), under the registration number: RBR-3vh8zgj.
Mitogen- and stress-activated protein kinase (MSK1/2) regulated gene expression in normal and disease states
Authors: Sattarifard Hedieh , Safaei Akram , Khazeeva Enzhe , Rastegar Mojgan , Davie James R.
Published: February 2023

The mitogen- and stress-activated protein kinases (MSK) are epigenetic modifiers that regulate gene expression in normal and disease cell states. MSK1 and 2 are involved in a chain of signal transduction events bringing signals from the external environment of a cell to specific sites in the genome. MSK1/2 phosphorylate histone H3 at multiple sites, resulting in chromatin remodeling at regulatory elements of target genes and the induction of gene expression. Several transcription factors (RELA of NF-κB and CREB) are also phosphorylated by MSK1/2 and contribute to induction of gene expression. In response to signal transduction pathways, MSK1/2 can stimulate genes involved in cell proliferation, inflammation, innate immunity, neuronal function, and neoplastic transformation. Abrogation of the MSK-involved signaling pathway is among the mechanisms by which pathogenic bacteria subdue the host’s innate immunity. Depending on the signal transduction pathways in play and the MSK-targeted genes, MSK may promote or hinder metastasis. Thus, depending on the type of cancer and genes involved, MSK overexpression may be a good or poor prognostic factor. In this review, we focus on mechanisms by which MSK1/2 regulate gene expression, and recent studies on their roles in normal and diseased cells.
Wave propagation in a circular channel: sloshing and resonance
Authors: Borcia Ion Dan , Richter Sebastian , Borcia Rodica , Schön Franz-Theo , Harlander Uwe , Bestehorn Michael
Published: February 2023

Surface wave resonance of a liquid (water) layer confined in a circular channel is studied both experimentally and numerically. For the experiment, eight unevenly distributed ultrasonic distance sensors measure the local height of the wave surface. The resonance curves show maxima only for odd multiples of the fundamental resonance frequency $$f_0$$

f
0

. We explained this behavior using a simple intuitive “ping-pong” like model. Collision of wave fronts can be observed for higher frequencies. Also, the wave reflection on the walls can be treated as wave collision with itself. The non-linearity seems to be weak in our study so the delay in the wave propagation before and after the collision is small. Time-space plots show localized propagating waves with high amplitudes for frequencies near resonance. Between the peaks low amplitude and harmonic patterns are observed. However, for higher frequencies, the frequency band for localized waves becomes wider. In the Fourier space-time plane, this can be observed as a point for the harmonic patterns or a superposition of two lines: one line parallel to wave-vector k axis corresponding to the excitation frequency $$f_0$$

f
0

and a second line with inclination given by wave propagation velocity $$\sqrt{gh}$$

gh

. For planned future work, this result will help us to reconstruct the whole water surface elevation using time-series from only a few measurement points
A Lightweight Three‐Party Mutual Authentication Protocol for Internet of Health Things Systems
Authors: Wang Zhihui , Zhao Jianli , Sun Peng , Yang Jingjing , Wang Rui , Zhang Xiao
Published: February 2023

In Internet of Health Things (IoHT) systems, there is a two‐hop network structure between the authentication server TA, Internet of Things Connector (IotC), and wearable sensor (WS). Attackers can use the sensor layer network (the first hop) between the IotC and WS to steal patient’s health‐related information and undermine the security of the system and the privacy of sensitive information. To address this threat, this study proposes a lightweight identity authentication and key agreement protocol for third‐party authentication servers TA, IotC, and WS. The results of the formal security proof, BAN logic analysis, and AVISPA tool simulation show that the scheme proposed in this study has an ideal security performance and can meet the security requirements of IoHT. In terms of performance, the proposed scheme could dynamically construct a sensor layer network (the first hop) and offline networking according to the diagnostic needs of doctors. Compared with other related protocols, the proposed scheme can significantly reduce the computing resource requirements of IotC and server TA and the resource requirements of database I/O operation of server TA in the application scenario of concurrent access of multiple WS nodes.
Leaders Condition the Work Experience: A Test of a Job Resources-Demands Model Invariance in Two Countries
Authors: Berger Rita , Glazer Sharon , Leiva David
Published: February 2023

Aim(s). This two-wave study examines how transformational and laissez-faire leadership affect role overload and conflict and subsequent outcomes, including anxiety and intention to leave the organization. Background. In today’s healthcare sector, promoting health among employees is more relevant than ever. Health-promoting leadership styles, such as transformational leadership, can positively affect staff well-being, but research on laissez-faire leadership is particularly sparse, though it is believed to be detrimental. Past research suggests that leadership conditions work experiences and can exacerbate or mitigate role stressors that result in individual outcomes. Method(s). Questionnaires were administered to nurses in the USA (n = 208) and Spain (n = 220), with a five- and eight-week separation, respectively. Results. Transformational leadership has a negative and laissez-faire leadership has a positive relationship with adverse outcomes. Furthermore, role overload and conflict mediate the relationship between leadership styles and outcomes. Conclusion(s). The study provides incremental evidence of the negative implications of laissez-faire leadership compared with the positive implications of transformational leadership on outcomes via role stressors as motivational mechanisms. Implications for Nursing Management. Learning about the medium-term implications of leadership styles on stressors and health-related outcomes would enrich opportunities for leadership training in organizations.