bluuebunny/crossref_metadata_2025 huggingface dataset:
Avian magnetic compass: Its functional properties and physical basis
Authors: Wiltschko Roswitha , Wiltschko Wolfgang
Published: September 2019

The avian magnetic compass was analyzed in bird species of three different orders – Passeriforms, Columbiforms and Galliforms – and in three different behavioral contexts, namely migratory orientation, homing and directional conditioning. The respective findings indicate similar functional properties: it is an inclination compass that works only within a functional window around the ambient magnetic field intensity; it tends to be lateralized in favor of the right eye, and it is wavelength-dependent, requiring light from the short-wavelength range of the spectrum. The underlying physical mechanisms have been identified as radical pair processes, spin-chemical reactions in specialized photopigments. The iron-based receptors in the upper beak do not seem to be involved. The existence of the same type of magnetic compass in only very distantly related bird species suggests that it may have been present already in the common ancestors of all modern birds, where it evolved as an all-purpose compass mechanism for orientation within the home range.
Identification and expression analysis of bovine ANGPTL gene family
Authors: Guan Zhengbing , Cai Guolin , Sun Junyong , Lu Jian
Published: September 2019

Encoded by seven genes, angiopoietin-like (ANGPTL) family members structurally similar to the angiogenic regulating factor angiopoietin are known to possess biological activities in angiogenesis and metabolism. Here we reports for the first time the identification and expression analysis of all the seven members of bovine ANGPTL gene family, which were designated bANGPTL1 to bANGPTL7 in order. The seven bANGPTL genes consist of 4-9 exons, span 3800-43000 bp and are located on different chromosomes. The deduced amino acid sequences of the members all possess an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins. Phylogenetic analysis showed that the 32 identified ANGPTL homologs from 9 species could be classified into two major groups. Real-time quantitative PCR (Q-PCR) analysis revealed that the bANGPTL family members have different expression patterns. This study will be helpful for investigation on the biological role of the bANGPTL family in this economically important species. Furthermore, it provides an insight into the molecular evolution of the emerging ANGPTL family.
Convergences in the diversification of bats
Authors: Fenton M. Brock
Published: September 2019

Twenty-five characters or suites of characters from bats are considered in light of changes in bat classification. The characters include some associated with flower-visiting (two), echolocation (12), roosting (six), reproduction (two) and three are of unknown adaptive function. In both the 1998 and 2006 classifications of bats into suborders (Megachiroptera and Microchiroptera versus Yinpterochiroptera and Yangochiroptera, respectively), some convergences between suborders are the same (e.g., foliage roosting, tent building), but others associated with echolocation differ substantially. In the 1998 phylogeny convergences associated with echolocation (high duty cycle echolocation, nasal emission of echolocation calls) occurred among the Microchiroptera. In the 2006 phylogeny, they occur between Yinpterochiroptera and Yangochiroptera. While some traits apparently arose independently in two suborders (e.g., foliage-roosting, tent building, low intensity echolocation calls, noseleafs, nasal emission of echolocation calls, high duty cycle echolocation behaviour), others appear to have been ancestral (roosting in narrow spaces, laryngeal echolocation, stylohyal-tympanic contact, oral emission of echolocation calls, and small litter size). A narrow profile through the chest is typical of bats reflecting the thoracic skeleton. This feature suggests that the ancestors of bats spent the day in small crevices. Features associated with laryngeal echolocation appear to be ancestral, suggesting that echolocation evolved early in bats but was subsequently lost in one yinpterochiropteran lineage.
Age and sex specific timing, frequency, and spatial distribution of horseshoe crab spawning in Delaware Bay: Insights from a large-scale radio telemetry array
Authors: Smith David R. , Lorne J. Brousseau , Mandt Mary T. , Millard Michael J.
Published: September 2019

To study horseshoe crab Limulus polyphemus spawning behavior and migration over a large-spatial extent (>100 km), we arrayed fixed station radio receivers throughout Delaware Bay and deployed radio transmitters and archival tags on adult horseshoe crabs prior to their spawning season. We tagged and released 160 females and 60 males in 2004 and 217 females in 2005. The array covered approximately 140 km of shoreline. Recapture rates were >70% with multi-year recaptures. We categorized adult age by carapace wear. Older females tended to spawn earlier in the season and more frequently than young females, but those tendencies were more apparent in 2004 when spawning overall occurred earlier than in 2005 when spawning was delayed possibly due to decreased water temperatures. Timing of initial spawning within a year was correlated with water temperature. After adjusting for day of first spring tide, the day of first spawning was 4 days earlier for every 1 degree (̊C) rise in mean daily water temperature in May. Seventy nine % of spawning occurred during nighttime high tides. Fifty five % of spawning occurred within 3 d of a spring tide, which was slightly higher than the 47% expected if spawning was uniformly distributed regardless of tidal cycle. Within the same spawning season, males and females were observed spawning or intertidally resting at more than one beach separated by >5 km. Between years, most (77%) did not return to spawn at the same beach. Probability of stranding was strongly age dependent for males and females with older adults experiencing higher stranding rates. Horseshoe crabs staging in the shallow waters east of the channel spawned exclusively along the eastern (NJ) shoreline, but those staging west of the channel spawned throughout the bay. Overall, several insights emerged from the use of radio telemetry, which advances our understanding of horseshoe crab ecology and will be useful in conserving the Delaware Bay horseshoe crab population and habitats.
Reproductive behavior in horseshoe crabs: Does density matter?
Authors: Mattei Jennifer H. , Beekey Mark A. , Rudman Adam , Woronik Alyssa
Published: September 2019

While the four species of horseshoe crabs share many common reproductive traits with respect to their reproductive systems, they do differ with respect to their mating behavior (monogamy vs. polygynandry). Past research has attributed these differences to a number of factors including: spawning densities, operational sex ratios (OSR's), male condition (or age), environmental and/or genetic factors, or a combination thereof. Mating behaviors in the three Asian horseshoe crab species (Tachypleus gigas, T. tridentatus, and Carcinoscorpius rotundicauda) with low spawning densities and 1:1 operational sex ratios are typically monogamous. In Limulus polyphemus, mating behavior is more variable ranging from monogamy to polygynandry. Here we provide evidence, through a long term behavioral study, that variation in mating behavior is influenced by population density in L. polyphemus. Our study population on two beaches in Connecticut (Long Island Sound) have a spawning density 400 times less than that found in Delaware Bay (0.002 females/m2 vs. 0.8 females/m2) but similar operational sex ratios. Between 90%-95% of all spawning females in CT were paired with only one male, thus exhibiting monogamous behavior. In contrast, between 30 and 60% of spawning females in Delaware Bay have more than one mate and produce clutches of eggs with multiple paternities. Male condition played no role in mating behavior in CT populations. We also observed that on average 18% of the females on the spawning beaches are single. These results suggest that population density is an important condition that determines mating behavior. Also, low population density may lead to decreased mate finding ability and lost opportunities for spawning.
Application of heat shock protein expression for detecting natural adaptation and exposure to stress in natural populations
Authors: Sørensen Jesper Givskov
Published: September 2019

Heat-shock proteins (HSPs) play an undisputed role for maintaining cellular functioning under environmental challenges and protein denaturing conditions. Compelling evidence points to an evolutionary important role of HSPs and a strict evolutionary control of these proteins as a balance between benefits and costs. While there is a great potential for using HSP expression for detecting natural adaptation and exposure to stress in natural populations, some obstacles and key issues await investigation. From an ecological perspective these key issues needs to be resolved in order to fully appreciate the complex responses and adaptations to stress and to increase our understanding of HSPs and other molecular chaperones for stress adaptation and potential use as biomarkers. Here, the current knowledge and understanding of HSPs is reviewed and a number of key issues including the interpretation of elevated HSP levels, the complications of extrapolating between laboratory and field conditions, the effects of choice of traits and methodology and the larger intra-and extracellular networks of interactions that HSPs participate in are discussed.
Primary neuroendocrine tumour of the left ventricle
Authors: Rogers Michael P , Thomas Sarah , Nallamshetty Leelakrishna , Hooker Robert
Published: September 2019

Neuroendocrine tumours are rare neoplasms typically arising in the gastrointestinal tract that may result in carcinoid syndrome and/or acquired valvular dysfunction. Herein, we present a unique case of a 68-year-old asymptomatic woman with a primary left ventricular neuroendocrine tumour.
Incidence, aetiology and outcomes of major postoperative haemorrhage after pulmonary lobectomy
Authors: Udelsman Brooks V , Soni Monica , Madariaga Maria Lucia , Fintelmann Florian J , Best Till D , Li Selena Shi-Yao , Chang David C , Gaissert Henning A
Published: September 2019

OBJECTIVES

Post-lobectomy bleeding is uncommon and rarely studied. In this study, we aimed to determine the incidence of post-lobectomy haemorrhage and compare the outcomes of reoperation and non-operative management.

METHODS

We conducted a single-institution review of lobectomy cases from 2009 to 2018. The patients were divided into two groups based on the treatment for postoperative bleeding: reoperation or transfusion of packed red blood cells with observation. Transfusion correcting intraoperative blood loss was excluded. One or more criteria defined postoperative bleeding: (i) drop in haematocrit ≥10 or (ii) frank, sustained chest tube bleeding with or without associated hypotension. Covariates included demographics, comorbidities and operative characteristics. Outcomes were operative mortality, complications, length of hospital stay and readmission within 30 days.

RESULTS

Following 1960 lobectomies (92% malignant disease, 8% non-malignant), haemorrhage occurred in 42 cases (2.1%), leading to reoperation in 27 (1.4%), and non-operative management in 15 (0.8%). The median time to reoperation was 17 h. No source of bleeding was identified in 44% of re-explorations. Patients with postoperative haemorrhage were more often male (64.3% vs 41.2%; P < 0.01) and more likely to have preoperative anaemia (45.2% vs 26.5%; P = 0.01), prior median sternotomy (14.3% vs 6.0%; P = 0.04), an infectious indication (7.1% vs 1.8%; P = 0.01) and operative adhesiolysis (45.2% vs 25.8%; P = 0.01). Compared with non-operative management, reoperation was associated with fewer units of packed red blood cells transfusion (0.4 vs 1.9; P < 0.001), while complication rates were similar and 30-day mortality was absent in either group.

CONCLUSIONS

Haemorrhage after lobectomy is associated with multiple risk factors. Reoperation may avoid transfusion. A prospective study should optimize timing and selection of operative and non-operative management.
If smallholder farmers have access to the world market: the case of tobacco marketing in Malawi
Authors: Zant Wouter
Published: September 2019

We measure the impact of a reduction in transaction costs on crop area and production of smallholder tobacco growers in Malawi. For identification, we exploit the introduction of an additional tobacco auction floor. Estimations are based on annual data by extension planning area. A 10 per cent reduction in distance to auction floor is shown to increase crop area and production around 4 and 10 per cent, respectively. Supply response weakens beyond a distance to auction floor of 60 km and runs along the intensive margin: existing tobacco growers improve productivity of cultivation. Impacts are robust for non-random placement of auction floors and several other threats. The results point to potentially large welfare benefits for smallholders if marketing infrastructure is improved and if more auction floors are introduced.
Does country-level gender equality explain individual risk of intimate partner violence against women? A multilevel analysis of individual heterogeneity and discriminatory accuracy (MAIHDA) in the European Union
Authors: Ivert Anna-Karin , Gracia Enrique , Lila Marisol , Wemrell Maria , Merlo Juan
Published: September 2019

Background

Gender equality is widely accepted as an important explanatory factor for the occurrence of intimate partner violence (IPV) against women. However, the relationship is not straightforward, as high country-level gender equality is not always associated with lower IPV prevalence. We apply ‘multilevel analysis of individual heterogeneity and discriminatory accuracy’ (MAIHDA) to (i) quantify the extent to which the country of residence determines individual risk of IPV and (ii) investigate the association between country-level gender equality and individual experience of IPV, and to which extent this association explains the observed between-country differences.

Methods

Using data from the 2012 European Union Agency for Fundamental Rights survey on violence against women we applied MAIHDA to analyse experiences of physical and sexual IPV among 42 000 women living in the EU. We fitted three consecutive models, and calculated specific individual contextual effects (measures of association) as well as the general contextual effects (measures of variance) and the discriminatory accuracy (DA).

Results

Our findings show that the relationship between experiences of IPV and country-level gender equality is weak and heterogeneous. The general contextual effect is small and the DA is low, indicating that country boundaries are rather irrelevant for understanding the individual risk of IPV.

Conclusions

Findings from the present study do not imply that that gender equality is unimportant in relation to IPV, but rather that information on country of residence or country-level gender equality does not discriminate very well with regards to individual experiences of IPV in cross-national comparisons.
Multicentre descriptive cross-sectional study of Japanese home visit patients: reasons for encounter, health problems and multimorbidity
Authors: Kaneko Makoto , Van Boven Kees , Takayanagi Hiroshi , Kusaba Tesshu , Yamada Takashi , Matsushima Masato
Published: September 2019

Background

GP in Japan are encouraged to conduct home visits for older adults. However, most previous studies on home visits were based on secondary analyses of billing data that did not include reasons for the encounter.

Objectives

This study aimed to describe home visit care by GP in Japan, including reasons for encounter, health problems, episodes of care, comprehensiveness and multimorbidity.

Methods

This multicentre descriptive cross-sectional study used the International Classification of Primary Care, second edition, and was conducted in Japan from 1 October 2016 to 31 March 2017. Participants were patients who received home visits from 10 enrolled GPs working in urban and rural areas across Japan. The main outcome measures were reasons for encounter, health problems and multimorbidity.

Results

Of 253 potential patient participants, 250 were included in this analysis; 92.4% were aged 65 years and older. We registered 1,278 regular home visits and 110 emergency home visits. The top three reasons for encounters home visits were associated with cardiovascular and gastrointestinal disorders: prescriptions for cardiovascular diseases (n = 796), medical examination/health evaluation for cardiovascular diseases (n = 758) and prescriptions for gastrointestinal problems (n = 554). About 50% of patients had multimorbidity. Cardiovascular, endocrine and neuropsychological diseases were the most frequent problems in patients with multimorbidity.

Conclusions

The main reasons for encounter were prescriptions for chronic conditions. Emergency visits accounted for 8% of all visits. Around half of the patients had multimorbidity. This information may help GPs and policy makers to better assess home visit patients' needs.
Photosynthetic capacity of the endosymbiotic dinoflagellate Cladocopium sp. is preserved during digestion of its jellyfish host Mastigias papua by the anemone Entacmaea medusivora
Authors: Vega de Luna Félix , Dang Kieu-Van , Cardol Mila , Roberty Stéphane , Cardol Pierre
Published: September 2019

The sea anemone Entacmaea medusivora (Actiniaria, Anthozoa) commonly feeds on the golden jellyfish Mastigias papua (Rhizostomeae, Scyphozoa) which harbours an endosymbiotic dinoflagellate of the genus Cladocopium (Symbiodiniaceae). In this study, we monitored the photosynthetic activity of the endosymbiotic microalgae while their host jellyfish were ingested and digested by starved medusivorous anemones. By analyzing the photosynthetic yield of photosystem II, we observed that Cladocopium cells remain photosynthetically competent during the whole digestion process, thus confirming the exceptional resistance of Symbiodiniaceae to digestive enzymes. In the gastric cavity of E. medusivora, Cladocopium cells release oxygen, which could broadly stimulate the gastric microbiotic flora of the sea anemone. Ultimately, E. medusivora is not able to retain Cladocopium cells more than few days and physiologically-unaltered cells are therefore expelled in faecal pellets. The potential contribution of E. medusivora to maintain a reservoir of Cladocopium symbionts and its role in the life cycle of M. papua is discussed.
Preceding crop and tillage system affect winter survival of wheat and the fungal communities on young wheat roots and in soil
Authors: Friberg Hanna , Persson Paula , Jensen Dan Funck , Bergkvist Göran
Published: September 2019

Agricultural practices like tillage and cropping sequence have profound influence on soil-living and plant-associated fungi, and thereby on plant growth. In a field experiment, we studied the effects of preceding crop and tillage on fungal communities in the soil and on young winter wheat roots in relation to plant winter survival and grain yield. We hypothesized that plant performance and fungal communities (described by amplicon sequencing) differ depending on tillage system and preceding crop; that the effect of preceding crop differs depending on tillage system, and that differences in fungal communities are reflected in plant performance. In line with our hypotheses, effects of preceding crop on plant growth and fungal communities on plant roots and in soil were more pronounced under non-inversion tillage than under inversion tillage (ploughing). Fungal communities on plant roots in treatments with low winter survival were different from those with better survival. In soil, several fungal OTUs (operational taxonomic units) differed significantly between tillage systems. OTUs representing putative plant pathogens were either more abundant (Parastagonospora sp._27) or less abundant (Fusarium culmorum/graminearum_5) after non-inversion tillage. Our findings highlight the influence of cultural practices on fungal communities and thereby on plant health and yield.
Heterologous expression of ctsR from Oenococcus oeni enhances the acid-ethanol resistance of Lactobacillus plantarum
Authors: Zhao Hongyu , Yuan Lin , Hu Kai , Liu Longxiang , Peng Shuai , Li Hua , Wang Hua
Published: September 2019

Oenococcus oeni is a lactic acid bacterium that is widely used in wine-making to conduct malolactic fermentation (MLF). During MLF, O. oeni undergoes acid and ethanol stress that impairs its growth. In order to investigate the role that the ctsR gene plays in acid-ethanol stress, the ctsR gene from O. oeni was expressed heterologously in Lactobacillus plantarum. The transcription level of the ctsR gene and 10 additional stress response genes in L. plantarum were analyzed by RT-qPCR. Physiological assays to assess reactive oxygen species accumulation, cell membrane integrity, intracellular ATP and GSH levels, Ca2+/Mg2+-ATPase and Na+/K+-ATPase activities were also performed. Results showed that the recombinant strain WCFS1-CtsR exhibited stronger growth performance than the control strain WCFS1-Vector, and the expression of ctsR, clp and hsp genes were significantly increased under acid-ethanol stress. Furthermore, WCFS1-CtsR displayed 1.08- and 1.39-fold higher ATP and GSH concentrations, respectively, compared with the corresponding values for WCFS1-Vector under acid-ethanol stress. ROS accumulation and PI value of WCFS1-CtsR were decreased by 46.52 and 42.80%, respectively, compared with the control strain. In addition, the two ATPase activities in WCFS1-CtsR increased significantly compared with WCFS1-Vector. This is the first report demonstrating that ctsR gene enhances the acid-ethanol tolerance of L. plantarum.
Is Bullying Victimization in Childhood Associated With Mental Health in Old Age
Authors: Hu Bo
Published: September 2019

Objective

This study investigates the relationship between bullying victimization in childhood and mental health in old age.

Methods

The study uses data from a nationally representative sample of 9,208 older people aged 60 and older collected through the China Health and Retirement Longitudinal Study (CHARLS) conducted in 2014 and 2015.

Results

Older people who were bullied in childhood have more severe depressive symptoms and are more likely to be dissatisfied with life than those without the experience of bullying victimization. The negative impacts remain significant after childhood confounders (15 types of familial adversities), four groups of contemporary confounders (demographic, health, social support, and socioeconomic factors), and community-level unobserved heterogeneity are all controlled for. The negative impacts of bullying victimization on mental health are attenuated among people in very old age, which confirms the socioemotional selectivity theory.

Discussion

The consequences of bullying victimization for mental health are comparable to, or even greater than those of familial adversities and contemporary risk factors. The factors threatening mental health vary considerably for older people in different age groups. Effective anti-bullying schemes in childhood and personalized support in later life can make a substantial contribution to healthy aging.
Effectiveness of Interventions for Preventing People With Dementia Exiting or Getting Lost
Authors: Emrich-Mills Luke , Puthusseryppady Vaisakh , Hornberger Michael
Published: September 2019

Background and Objectives

People with dementia are at risk of exiting premises unsupervised, eloping, or getting lost, potentially leading to harmful or distressing consequences. This review aimed to estimate the effectiveness of interventions for preventing people with dementia from exiting or getting lost.

Research Design and Methods

A systematic review of English sources was undertaken. Health care (EMBASE, BNI, Medline, PubMed, CINAHL, PsycINFO, AMED, HTA, CENTRAL) and gray literature (OpenGrey) databases were searched using prespecified search terms. Additional studies were identified by hand-searching bibliographies of relevant reviews and included studies. Wide inclusion criteria were set to capture a range of intervention types. Data extraction and risk of bias assessment were completed independently by two reviewers. Methods were preregistered on PROSPERO.

Results

Individual and overall risk of bias was too high for statistical meta-analyses. A narrative synthesis was therefore performed. Twenty-five studies with 814 participants were included, investigating a range of nonpharmacological interventions aiming to prevent exiting, facilitate retrieval, educate participants, or a combination of these. Seventeen (68%) of the included studies had critical risks of internal bias to outcomes, providing no useful evidence for the effectiveness of their respective interventions. The remaining 8 (32%) studies had serious risks of bias. Narrative synthesis of results yielded no overall robust evidence for the effectiveness of any interventions.

Discussion and Implications

No evidence was found to justify the recommendation of any interventions included in this review. Future studies should focus on high-quality, controlled study designs.
Determinants of Self-rated Cognitive Health among Older Korean Americans
Authors: Jang Yuri , Choi Eun Young , Rhee Min-Kyoung , Park Nan Sook , Chiriboga David A , Kim Miyong T
Published: September 2019

Background and Objectives

In response to the dearth of information on cognitive health in older ethnic minorities, in the present study, we examined factors associated with self-rated cognitive health (SRCH) in older Korean Americans. Drawing from the World Health Organization’s framework of social determinants of health, we examined how a broad spectrum of factors might influence the way in which older Korean Americans perceive and evaluate their own cognitive health.

Research Design and Methods

Using data from the Study of Older Korean Americans (SOKA; N = 2,061, mean age = 73.2), a series of hierarchical linear regression models of SRCH was tested with sequential entry of predictors: (1) Mini-Mental State Examination (MMSE) score of cognitive status, (2) demographic variables, (3) physical and mental health status, (4) health behaviors, and (5) socio-cultural and community factors.

Results

In addition to cognitive screening outcomes, each set of variables made a significant contribution to the predictive model of SRCH. Positive ratings of cognitive health were observed among older individuals with greater education, good physical and mental health, involvement in regular exercise, and socio-cultural resources (social networks, acculturation, and family solidarity).

Discussion and Implications

The SRCH of older adults goes beyond cognitive screening outcomes, suggesting a need to incorporate the various sources of social determinants when seeking to promote the cognitive health of older populations.
Validation of a fast semi-analytic method for surface-wave propagation in layered media
Authors: Brissaud Quentin , Tsai Victor C
Published: September 2019

Green’s functions provide an efficient way to model surface-wave propagation and estimate physical quantities for near-surface processes. Several surface-wave Green’s function approximations (far-field, no mode conversions and no higher mode surface waves) have been employed for numerous applications such as estimating sediment flux in rivers, determining the properties of landslides, identifying the seismic signature of debris flows or to study seismic noise through cross-correlations. Based on those approximations, simple empirical scalings exist to derive phase velocities and amplitudes for pure power-law velocity structures providing an exact relationship between the velocity model and the Green’s functions. However, no quantitative estimates of the accuracy of these simple scalings have been reported for impulsive sources in complex velocity structures. In this paper, we address this gap by comparing the theoretical predictions to high-order numerical solutions for the vertical component of the wavefield. The Green’s functions computation shows that attenuation-induced dispersion of phase and group velocity plays an important role and should be carefully taken into account to correctly describe how surface-wave amplitudes decay with distance. The comparisons confirm the general reliability of the semi-analytic model for power-law and realistic shear velocity structures to describe fundamental-mode Rayleigh waves in terms of characteristic frequencies, amplitudes and envelopes. At short distances from the source, and for large near-surface velocity gradients or high Q values, the low-frequency energy can be dominated by higher mode surface waves that can be captured by introducing additional higher mode Rayleigh-wave power-law scalings. We also find that the energy spectral density for realistic shear-velocity models close to piecewise power-law models can be accurately modelled using the same non-dimensional scalings. The frequency range of validity of each power-law scaling can be derived from the corresponding phase velocities. Finally, highly discontinuous near-surface velocity profiles can also be approximated by a combination of power-law scalings. Analytical Green’s functions derived from the non-dimensionalization provide a good estimate of the amplitude and variations of the energy distribution, although the predictions are quite poor around the frequency bounds of each power-law scaling.
Anisotropy as an indicator for reservoir changes: example from the Rotokawa and Ngatamariki geothermal fields, New Zealand
Authors: Mroczek Stefan , Savage Martha K , Hopp Chet , Sewell Steven M
Published: September 2019

We investigate the relation between geothermal field production and fracture density and orientation in the Ngatamariki and Rotokawa geothermal fields, located in the Taupo Volcanic Zone, New Zealand using shear wave splitting (SWS). We determine the SWS parameters for 17 702 microseismic events across 38 stations spanning close to 4 yr from 2012 to 2015. We compare the strength of anisotropy to changes in field production and injection. We also compare the orientation of the anisotropy to in situ and regional measurements of maximum horizontal stress orientation. ($S_{\mathrm{ H}_{\mathrm{ max}}}$). Due to the volume of unique events (approximately 160 000), shear wave phases are picked automatically. We carry out automatic SWS measurements using the Multiple Filter Automatic Splitting Technique (MFAST). The SWS measurements are interpreted in the context of stress aligned microcracks. Outside both fields and within Ngatamariki, fast polarizations align with the NE–SW regional orientation of $S_{\mathrm{ H}_{\max}}$. Within Rotokawa a greater complexity is observed, with polarizations tending toward N–S. We observe increases in per cent anisotropy coinciding with the start of production/injection in Ngatamariki and then a later correlated drop in per cent anisotropy and vP/vS ratios in southern Ngatamariki as injection is shifted to the north. This relationship is consistent with pore fluid pressure within the reservoir being affected by local changes in production and injection of geothermal fluids causing cracks to open and close in response.
Imaging zero-offset 3-D P-cable data with CRS method
Authors: Glöckner M , Walda J , Dell S , Gajewski D , Karstens J , Kläschen D , Berndt C
Published: September 2019

Standard seismic acquisition and processing require appropriate source–receiver offsets. P-cable technology represents the opposite, namely, very short source–receiver offsets at the price of increased spatial and lateral resolution with a high-frequency source. To use this advantage, a processing flow excluding offset information is required. This aim can be achieved with a processing tuned to diffractions because point diffractions scatter the same information in the offset and midpoint direction. Usually, diffractions are small amplitude events and a careful diffraction separation is required as a first step. We suggest the strategy to use a multiparameter stacking operator, for example, common-reflection surface, and stack along the midpoint direction. The obtained kinematic wave-front attributes are used to calculate time-migration velocities. A diffractivity map serves as a filter to refine the velocities. This strategy is applied to a 3-D P-cable data set to obtain a time-migrated image.
Inequalities in the benefits of national health insurance on financial protection from out-of-pocket payments and access to health services: cross-sectional evidence from Ghana
Authors: Fiestas Navarrete Lucia , Ghislandi Simone , Stuckler David , Tediosi Fabrizio
Published: September 2019

A central pillar of universal health coverage (UHC) is to achieve financial protection from catastrophic health expenditure. There are concerns, however, that national health insurance programmes with premiums may not benefit impoverished groups. In 2003, Ghana became the first sub-Saharan African country to introduce a National Health Insurance Scheme (NHIS) with progressively structured premium charges. In this study, we test the impact of being insured on utilization and financial risk protection compared with no enrolment, using the 2012–13 Ghana Living Standards Survey (n = 72 372). Consistent with previous studies, we observed that participating in health insurance significantly decreased the probability of unmet medical needs by 15 percentage points (p.p.) and that of incurring catastrophic out-of-pocket (OOP) health payments by 7 p.p. relative to no enrolment in the NHIS. Households living outside a 1-h radius to the nearest hospital had lower reductions in financial risk from excess OOP medical spending relative to households living closer (−5 p.p. vs −9 p.p.). We also find evidence that in Ghana, the scheme was highly pro-poor. Once insured, the poorest 40% of households experienced significantly larger improvements in medical utilization (18 p.p. vs. 8 p.p.) and substantively larger reductions in catastrophic OOP health expenditure (−10 p.p. vs. −6 p.p.) compared with that of the richest households. However, health insurance did not benefit vulnerable persons equally from financial risk. Once insured, poor, low-educated and self-employed households living far from hospitals had significantly lower reductions in catastrophic OOP medical spending compared with their counterparts living closer. Taken together, we show that enrolment in the NHIS is associated with improved financial protection but less so among geographically remote vulnerable groups. Efforts to boost not just insurance uptake but also health service delivery may be needed as a supplement for insurance schemes to accelerate progress towards UHC.
The impact of reimbursed user fee exemption of health centre outpatient consultations for the poor in pluralistic health systems: lessons from a quasi-experiment in two rural health districts in Cambodia
Authors: Korachais Catherine , Ir Por , Macouillard Elodie , Meessen Bruno
Published: September 2019

Fees charged at the point of use are a barrier to the health services’ users, especially for the poorest. Two decades ago, Cambodia introduced the so-called health equity fund (HEF) strategy, a waiver scheme which enhances access to public health services for the poor without undermining the economic situation of facilities. Evidence suggests that hospital-based HEF effectively removed financial barriers and reduced out-of-pocket expenditures. There is less evidence on the effectiveness of the HEF when assistance is extended to the primary level of healthcare. This research explores the impact of a HEF extended to health centres in two rural health districts. Two household surveys and 16-month diary data allowed to assess the impact of the intervention on health-seeking behaviours and expenditure of poor households. Though HEF effectively removed user fees at public health facilities, health centre utilization of sick and poor people did not budge much in the intervention district; self-medication and private provider consultations remained the preferred health-seeking behaviours, by far, even if more expensive. Difference-in-difference estimates confirmed that HEF had a slight impact on health-seeking behaviours, but only for the subgroups of HEF beneficiaries living close to the health centre and ready to test their new entitlement. This research reminds on the importance of the context for the effectiveness of any policy: in a highly pluralistic health sector, waiving already low-user fees in public health centres may be insufficient to increase rapidly the use of those facilities and reduce catastrophic spending. In such context, apart from distance to health centres, perceived quality of services at the health centres, which was relatively low compared with other providers, also matters. Although the HEF scheme plays a role in improving perceived and objective quality of care, complementary means are to be deployed.
Individualized management of genetic diversity in Niemann-Pick C1 through modulation of the Hsp70 chaperone system
Authors: Wang Chao , Scott Samantha M , Sun Shuhong , Zhao Pei , Hutt Darren M , Shao Hao , Gestwicki Jason E , Balch William E
Published: September 2019

Genetic diversity provides a rich repository for understanding the role of proteostasis in the management of the protein fold in human biology. Failure in proteostasis can trigger multiple disease states, affecting both human health and lifespan. Niemann-Pick C1 (NPC1) disease is a rare genetic disorder triggered by mutations in NPC1, a multi-spanning transmembrane protein that is trafficked through the exocytic pathway to late endosomes (LE) and lysosomes (Ly) (LE/Ly) to globally manage cholesterol homeostasis. Defects triggered by >300 NPC1 variants found in the human population inhibit export of NPC1 protein from the endoplasmic reticulum (ER) and/or function in downstream LE/Ly, leading to cholesterol accumulation and onset of neurodegeneration in childhood. We now show that the allosteric inhibitor JG98, that targets the cytosolic Hsp70 chaperone/co-chaperone complex, can significantly improve the trafficking and post-ER protein level of diverse NPC1 variants. Using a new approach to model genetic diversity in human disease, referred to as variation spatial profiling, we show quantitatively how JG98 alters the Hsp70 chaperone/co-chaperone system to adjust the spatial covariance (SCV) tolerance and set-points on an amino acid residue-by-residue basis in NPC1 to differentially regulate variant trafficking, stability, and cholesterol homeostasis, results consistent with the role of BCL2-associated athanogene family co-chaperones in managing the folding status of NPC1 variants. We propose that targeting the cytosolic Hsp70 system by allosteric regulation of its chaperone/co-chaperone based client relationships can be used to adjust the SCV tolerance of proteostasis buffering capacity to provide an approach to mitigate systemic and neurological disease in the NPC1 population.
FUS-mediated dysregulation of Sema5a, an autism-related gene, in FUS mice with hippocampus-dependent cognitive deficits
Authors: Ho Wan Yun , Chang Jer-Cherng , Tyan Sheue-Houy , Yen Yi-Chun , Lim Kenneth , Tan Bernice Siu Yan , Ong Jolynn , Tucker-Kellogg Greg , Wong Peiyan , Koo Edward , Ling Shuo-Chien
Published: September 2019

Pathological fused in sarcoma (FUS) inclusions are found in 10% of patients with frontotemporal dementia and those with amyotrophic lateral sclerosis (ALS) carrying FUS mutations. Current work indicates that FUS mutations may incur gain-of-toxic functions to drive ALS pathogenesis. However, how FUS dysfunction may affect cognition remains elusive. Using a mouse model expressing wild-type human FUS mimicking the endogenous expression pattern and level within the central nervous system, we found that they developed hippocampus-mediated cognitive deficits accompanied by an age-dependent reduction in spine density and long-term potentiation in their hippocampus. However, there were no apparent FUS aggregates, nuclear envelope defects and cytosolic FUS accumulation. These suggest that these proposed pathogenic mechanisms may not be the underlying causes for the observed cognitive deficits. Unbiased transcriptomic analysis identified expression changes in a small set of genes with preferential expression in the neurons and oligodendrocyte lineage cells. Of these, we focused on Sema5a, a gene involved in axon guidance, spine dynamics, Parkinson’s disease and autism spectrum disorders. Critically, FUS binds directly to Sema5a mRNA and regulates Sema5a expression in a FUS-dose-dependent manner. Taken together, our data suggest that FUS-driven Sema5a deregulation may underlie the cognitive deficits in FUS transgenic mice.
Duplicate and salami publication: a prevalence study of journal policies
Authors: Ding Ding , Nguyen Binh , Gebel Klaus , Bauman Adrian , Bero Lisa
Published: September 2019

Background

Duplicate and salami publication are unethical, but are common practices with substantial consequences for science and society at large. Scientific journals are the ‘gatekeepers’ of the publication process. We investigated journal policies on duplicate and salami publication.

Methods

In 2018, we performed a content analysis of policies of journals in the disciplines of ‘epidemiology and public health’ and ‘general and internal medicine’. Journal policies were searched, extracted, coded and cross-checked. The associations of disciplinary categories and journal impact factors with journal policies were examined using Poisson regression models with a robust error variance.

Results

A total of 209 journals, including 122 in epidemiology and public health and 87 in general and internal medicine, were sampled and their policies investigated. Overall, 18% of journals did not have any policies on either practice, 33% only referred to a generic guideline or checklist without explicit mention about either practice, 36% included policies on duplicate publication and only 13% included policies on both duplicate and salami publication. Having explicit journal policies did not differ by journal disciplinary categories (epidemiology and public health vs general and internal medicine) or impact factors. Further analysis of journals with explicit policies found that although duplicate publication is universally discouraged, policies on salami publication are inconsistent and lack specific definitions of inappropriate divisions of papers.

Conclusions

Gaps exist in journal policies on duplicate and salami publication, characterized by an overall lack of explicit policies, inconsistency and confusion in definitions of bad practices, and lack of clearly defined consequences for non-compliance. Scientific publication and the academic reward systems must evolve to credit good research practice.
Appraising the causal relevance of DNA methylation for risk of lung cancer
Authors: Battram Thomas , Richmond Rebecca C , Baglietto Laura , Haycock Philip C , Perduca Vittorio , Bojesen Stig E , Gaunt Tom R , Hemani Gibran , Guida Florence , Carreras-Torres Robert , Hung Rayjean , Amos Christopher I , Freeman Joshua R , Sandanger Torkjel M , Nøst Therese H , Nordestgaard Børge G , Teschendorff Andrew E , Polidoro Silvia , Vineis Paolo , Severi Gianluca , Hodge Allison M , Giles Graham G , Grankvist Kjell , Johansson Mikael B , Johansson Mattias , Davey Smith George , Relton Caroline L
Published: September 2019

Background

DNA methylation changes in peripheral blood have recently been identified in relation to lung cancer risk. Some of these changes have been suggested to mediate part of the effect of smoking on lung cancer. However, limitations with conventional mediation analyses mean that the causal nature of these methylation changes has yet to be fully elucidated.

Methods

We first performed a meta-analysis of four epigenome-wide association studies (EWAS) of lung cancer (918 cases, 918 controls). Next, we conducted a two-sample Mendelian randomization analysis, using genetic instruments for methylation at CpG sites identified in the EWAS meta-analysis, and 29 863 cases and 55 586 controls from the TRICL-ILCCO lung cancer consortium, to appraise the possible causal role of methylation at these sites on lung cancer.

Results

Sixteen CpG sites were identified from the EWAS meta-analysis [false discovery rate (FDR) < 0.05], for 14 of which we could identify genetic instruments. Mendelian randomization provided little evidence that DNA methylation in peripheral blood at the 14 CpG sites plays a causal role in lung cancer development (FDR > 0.05), including for cg05575921-AHRR where methylation is strongly associated with both smoke exposure and lung cancer risk.

Conclusions

The results contrast with previous observational and mediation analysis, which have made strong claims regarding the causal role of DNA methylation. Thus, previous suggestions of a mediating role of methylation at sites identified in peripheral blood, such as cg05575921-AHRR, could be unfounded. However, this study does not preclude the possibility that differential DNA methylation at other sites is causally involved in lung cancer development, especially within lung tissue.
Spatiotemporal Analysis of Serogroup C Meningococcal Meningitis Spread in Niger and Nigeria and Implications for Epidemic Response
Authors: Cooper Laura V , Ronveaux Olivier , Fernandez Katya , Lingani Clement , Goumbi Kadade , Ihekweazu Chikwe , Preziosi Marie-Pierre , Durupt Antoine , Trotter Caroline L
Published: September 2019

Background

After the re-emergence of serogroup C meningococcal meningitis (MM) in Nigeria and Niger, we aimed to re-evaluate the vaccination policy used to respond to outbreaks of MM in the African meningitis belt by investigating alternative strategies using a lower incidence threshold and information about neighboring districts.

Methods

We used data on suspected and laboratory-confirmed cases in Niger and Nigeria from 2013 to 2017. We calculated global and local Moran’s I-statistics to identify spatial clustering of districts with high MM incidence. We used a Pinner model to estimate the impact of vaccination campaigns occurring between 2015 and 2017 and to evaluate the impact of 3 alternative district-level vaccination strategies, compared with that currently used.

Results

We found significant clustering of high incidence districts in every year, with local clusters around Tambuwal, Nigeria in 2013 and 2014, Niamey, Niger in 2016, and in Sokoto and Zamfara States in Nigeria in 2017.

We estimate that the vaccination campaigns implemented in 2015, 2016, and 2017 prevented 6% of MM cases. Using the current strategy but with high coverage (85%) and timely distribution (4 weeks), these campaigns could have prevented 10% of cases. This strategy required the fewest doses of vaccine to prevent a case. None of the alternative strategies we evaluated were more efficient, but they would have prevented the occurrence of more cases overall.

Conclusions

Although we observed significant spatial clustering in MM in Nigeria and Niger between 2013 and 2017, there is no strong evidence to support a change in methods for epidemic response in terms of lowering the intervention threshold or targeting neighboring districts for reactive vaccination.