bluuebunny/crossref_metadata_2025 huggingface dataset:
Chronic Central Leptin Infusion Promotes an Anti-Inflammatory Cytokine Profile Related to the Activation of Insulin Signaling in the Gastrocnemius of Male Rats
Authors: Barrios Vicente , Guerra-Cantera Santiago , Martín-Rivada Álvaro , Canelles Sandra , Campillo-Calatayud Ana , Arilla-Ferreiro Eduardo , Frago Laura M. , Chowen Julie A. , Argente Jesús
Published: June 2022

Leptin is involved in the modulation of insulin signaling in peripheral tissues, being closely associated with changes in lipid metabolism. This adipokine modifies inflammatory pathways that can interact with insulin targets in peripheral organs; however, the mechanisms remain unclear. Inflammatory and insulin signaling targets, cytokines, adiponectin, irisin and non-esterified fatty acid (NEFA) levels and enzymes of fatty acid anabolism were studied in the gastrocnemius of chronic centrally infused leptin (L), pair-fed and control rats. The phosphorylation of signal transducer and activator of transcription 3 (STAT3) and c-Jun N-terminal kinase (JNK) was reduced in L rats (59% and 58%, respectively). The phosphorylation of the insulin receptor and Akt and adiponectin and irisin content was increased in L rats (154%, 157%, 308% and 329%, respectively). The levels of glucose-6-phosphate dehydrogenase, the mRNA content of acetyl Co-A carboxylase and NEFA concentrations were diminished in the muscles of L rats (59%, 50% and 61%, respectively). The activation of JNK correlated positively with STAT3 phosphorylation, tumoral necrosis factor-α and NEFA and negatively with irisin and Akt phosphorylation. These data suggest that the activation of insulin signaling targets and a decrease in NEFA content are associated with a reduction in muscle inflammation parameters, suggesting that leptin may integrate these pathways.
Intra-Individual Comparison of 124I-PET/CT and 124I-PET/MR Hybrid Imaging of Patients with Resected Differentiated Thyroid Carcinoma: Aspects of Attenuation Correction
Authors: Grafe Hong , Lindemann Maike E. , Weber Manuel , Kirchner Julian , Binse Ina , Umutlu Lale , Herrmann Ken , Quick Harald H.
Published: June 2022

Background: This study evaluates the quantitative differences between 124-iodine (I) positron emission tomography/computed tomography (PET/CT) and PET/magnetic resonance imaging (PET/MR) in patients with resected differentiated thyroid carcinoma (DTC). Methods: N = 43 124I PET/CT and PET/MR exams were included. CT-based attenuation correction (AC) in PET/CT and MR-based AC in PET/MR with bone atlas were compared concerning bone AC in the head-neck region. AC-map artifacts (e.g., dentures) were noted. Standardized uptake values (SUV) were measured in lesions in each PET data reconstruction. Relative differences in SUVmean were calculated between PET/CT and PET/MR with bone atlas. Results: Overall, n = 111 124I-avid lesions were detected in all PET/CT, while n = 132 lesions were detected in PET/MR. The median in SUVmean for n = 98 congruent lesions measured in PET/CT was 12.3. In PET/MR, the median in SUVmean was 16.6 with bone in MR-based AC. Conclusions: 124I-PET/CT and 124I-PET/MR hybrid imaging of patients with DTC after thyroidectomy provides overall comparable quantitative results in a clinical setting despite different patient positioning and AC methods. The overall number of detected 124I-avid lesions was higher for PET/MR compared to PET/CT. The measured average SUVmean values for congruent lesions were higher for PET/MR.
Maternal Pre-Pregnancy Obesity and Gestational Diabetes Mellitus Increase the Risk of Childhood Obesity
Authors: Choi Mi Jin , Yu Juyoun , Choi Jimi
Published: June 2022

Previous studies have shown inconsistent results regarding the effects of maternal gestational diabetes mellitus (GDM) and pre-pregnancy obesity (PPO) on childhood obesity. This study aimed to determine the risk for early childhood obesity based on maternal GDM and PPO. This nationwide study used data obtained from the National Health Information Database in South Korea. The participants were divided into four groups based on maternal GDM and PPO, and 1:1 matching was performed. Each group had 1319 participants. A generalized estimating equation model was used to analyze the changes in body mass index percentile of children with age, and simple and multiple conditional logistic regression models were used to compare the prevalence of childhood obesity at 5 years. Children whose mothers had both PPO and GDM, only PPO, or only GDM had a 4.46 (95% CI: 3.28–6.05, p < 0.001), 3.11 (95% CI: 2.27–4.26, p < 0.001), or 1.58 (95% CI: 1.12–2.23, p = 0.010) times higher risk, respectively, of developing childhood obesity than children whose mothers had neither PPO nor GDM. Maternal PPO increases the risk for childhood obesity to a higher degree than maternal GDM, and the presence of both increases the risk even further.
Risk Factors for Macrosomia in Multipara: A Multi-Center Retrospective Study
Authors: Juan Juan , Wei Yumei , Song Geng , Su Rina , Chen Xu , Shan Ruiqin , Yan Jianying , Xiao Mei , Li Ying , Cui Shihong , Zhao Xianlan , Fan Shangrong , Feng Ling , Zhang Meihua , Ma Yuyan , You Zishan , Meng Haixia , Liu Haiwei , Sun Jingxia , Cai Yan , Hu Kejia , Yang Huixia
Published: June 2022

The increased incidence of macrosomia has caused an enormous burden after the transition from the almost 40-year one-child policy to the universal two-child policy in 2015 and further to the three-child policy in 2021 in China. However, studies on risk factors of macrosomia in multipara under the new fertility policy in China are limited. We aim to explore the incidence and risk factors for macrosomia in multipara to provide the scientific basis for preventing macrosomia in multipara. A multi-center retrospective study was conducted among 6200 women who had two consecutive deliveries in the same hospital and their second newborn was delivered from January to October 2018 at one of 18 hospitals in 12 provinces in China. Macrosomia was defined as birth weight ≥ 4000 g. Logistic regression models were performed to analyze risk factors for macrosomia in multipara. The incidence of macrosomia in multipara was 7.6% (470/6200) and the recurrence rate of macrosomia in multipara was 27.2% (121/445). After adjusting for potential confounders, a higher prepregnancy BMI, higher gestational weight gain, history of macrosomia, a longer gestation in the subsequent pregnancy were independent risk factors of macrosomia in multipara (p < 0.05). Healthcare education and preconception consultation should be conducted for multipara patients with a history of macrosomia to promote maintaining optimal prepregnancy BMI and avoid excessive gestational weight gain to prevent macrosomia.
Correction: Kim et al. Assessing Role of Drought Indices in Anticipating Pine Decline in the Sierra Nevada, CA. Climate 2022, 10, 72
Authors: Kim Yoonji , Grulke Nancy E. , Merschel Andrew G. , Uyeda Kellie A.
Published: June 2022

Author “David A [...]
Health-Related Quality of Life following Total Thyroidectomy and Lobectomy for Differentiated Thyroid Carcinoma: A Systematic Review
Authors: Landry Vivianne , Siciliani Elizabeth , Henry Melissa , Payne Richard J.
Published: June 2022

Health-related quality of life (HrQoL) is a major concern for patients with differentiated thyroid carcinoma (DTC). We aimed to systematically review the literature comparing HrQol following total thyroidectomy (TT) and hemithyroidectomy (HT) in DTC patients. A systematic review of publications indexed in Medline, Embase, and EBM reviews—Cochrane Central Register of Controlled Trials, which evaluated HrQoL following thyroid surgery for DTC, was conducted. Of 2507 identified records, 25 fulfilled the inclusion criteria. Our results suggest that patients undergoing TT may suffer more impairment in physical and social HrQoL than patients undergoing HT. Psychological-related HrQoL and long-term global HrQoL are, however, equivalent in both groups, which highlights the multidimensional nature of HrQoL and the importance of a multitude of factors aside from treatment modalities and related morbidities, such as the experience of receiving a cancer diagnosis, the fear of cancer recurrence, and other psychosocial factors. Addressing postoperative HrQoL when discussing therapeutic options with patients is an integral part of patient-centered care and informed shared decision-making, and should be approached in a holistic manner, accounting for its physical, psychological, and social aspects. This review supplies evidence regarding HrQoL following thyroid surgery, which can be employed in such decisions.
Entropy-Weight-Method-Based Integrated Models for Short-Term Intersection Traffic Flow Prediction
Authors: Qu Wenrui , Li Jinhong , Song Wenting , Li Xiaoran , Zhao Yue , Dong Hanlin , Wang Yanfei , Zhao Qun , Qi Yi
Published: June 2022

Three different types of entropy weight methods (EWMs), i.e., EWM-A, EWM-B, and EWM-C, have been used by previous studies for integrating prediction models. These three methods use very different ideas on determining the weights of individual models for integration. To evaluate the performances of these three EWMs, this study applied them to developing integrated short-term traffic flow prediction models for signalized intersections. At first, two individual models, i.e., a k-nearest neighbors (KNN)-algorithm-based model and a neural-network-based model (Elman), were developed as individual models to be integrated using EWMs. These two models were selected because they have been widely used for traffic flow prediction and have been approved to be able to achieve good performance. After that, three integrated models were developed by using the three different types of EWMs. The performances of the three integrated models, as well as the individual KNN and Elman models, were compared. We found that the traffic flow predicted with the EWM-C model is the most accurate prediction for most of the days. Based on the model evaluation results, the advantages of using the EWM-C method were deliberated and the problems with the EWM-A and EWM-B methods were also discussed.
A Complex-Valued Self-Supervised Learning-Based Method for Specific Emitter Identification
Authors: Zhao Dongxing , Yang Junan , Liu Hui , Huang Keju
Published: June 2022

Specific emitter identification (SEI) refers to distinguishing emitters using individual features extracted from wireless signals. The current SEI methods have proven to be accurate in tackling large labeled data sets at a high signal-to-noise ratio (SNR). However, their performance declines dramatically in the presence of small samples and a significant noise environment. To address this issue, we propose a complex self-supervised learning scheme to fully exploit the unlabeled samples, comprised of a pretext task adopting the contrastive learning concept and a downstream task. In the former task, we design an optimized data augmentation method based on communication signals to serve the contrastive conception. Then, we embed a complex-valued network in the learning to improve the robustness to noise. The proposed scheme demonstrates the generality of handling the small and sufficient samples cases across a wide range from 10 to 400 being labeled in each group. The experiment also shows a promising accuracy and robustness where the recognition results increase at 10–16% from 10–15 SNR.
Scalability and Replicability for Smart Grid Innovation Projects and the Improvement of Renewable Energy Sources Exploitation: The FLEXITRANSTORE Case
Authors: Fotis Georgios , Dikeakos Christos , Zafeiropoulos Elias , Pappas Stylianos , Vita Vasiliki
Published: June 2022

In this paper, detailed scalability and replicability plans have been developed to facilitate the adoption of innovation technologies in the pan-EU market. Smart grid development must enable both information and power exchange between suppliers and customers, thanks to the enormous innovation in intelligent communication, monitoring, and management systems. Implementing physical infrastructure alone is not enough, but a smart grid must include new business models and new regulations. In recent years, the number, participants, and scope of smart grid initiatives have increased, with different goals and results. FLEXITRANSTORE project integrates hardware and software solutions in all areas of the transmission system and wholesale markets, unleashing the potential for full flexibility of power systems and promoting the penetration of renewable energy sources and pan-EU markets. Full deployment of these demonstrated solutions requires a reasonable level of scalability and replicability to prevent project demonstrators from continuing local experimental exercises. Scalability and replicability are fundamental requirements for successful scaling-up and replication. Therefore, scalability and replicability enable or at least reduce barriers to the growth and reuse of project demonstrator results.
Characterizing Various Produced Waters from Shale Energy Extraction within the Context of Reuse
Authors: Liden Tiffany , Hildenbrand Zacariah L. , Sanchez-Rosario Ramon , Schug Kevin A.
Published: June 2022

Environmental concerns with unconventional oil and gas development are frequently centered on elevated water usage and the induction of seismic events during waste disposal. Reuse of produced water for subsequent production well stimulation can effectively address these concerns, but the variability among such samples must be well understood. Twenty-four samples of wastewater from unconventional oil and gas development were collected from south and west Texas to assess their variability and feasibility for direct reuse. Bulk metrics were collected, including total organic carbon, total nitrogen, as well as total dissolved and suspended solids. The profiles of pertinent inorganic constituents were also evaluated. Variations were not only seen between regions but also among samples collected from the same region. For example, the average total organic carbon for Eagle Ford samples collected was 700 ± 500 mg/L, while samples collected from the Permian Basin featured an average total organic carbon concentration of 600 ± 900 mg/L. The Permian Basin total organic carbon ranged from 38 to 2600 mg/L. The total dissolved solids levels had the same variability between regions, with an average value for Eagle Ford of 20,000 ± 10,000 mg/L and a Permian Basin value of 150,000 ± 40,000 mg/L. However, samples were more reproducible within a given region. Collectively, the data indicate that the direct reuse of raw produced water for subsequent production well development without treatment is not feasible based on the reported reuse thresholds. Unconventional development wastewater samples from the Permian Basin were also compared to produced water values from conventional oil and gas wells in the same region, as reported by the United States Geological Survey. Samples collected in the Permian Basin consistently demonstrated lower ionic strength compared to conventional produced water data.
An Energy Cost Assessment of Future Energy Scenarios: A Case Study on San Pietro Island
Authors: Vargiu Alberto , Novo Riccardo , Moscoloni Claudio , Giglio Enrico , Giorgi Giuseppe , Mattiazzo Giuliana
Published: June 2022

The need for a clean and affordable energy supply is a major challenge of the current century. The tough shift toward a sustainable energy mix becomes even more problematic when facing realities that lack infrastructures and financing, such as small islands. Energy modeling and planning is crucial at this early stage of the ecological transition. For this reason, this article aims to improve an established long-run energy model framework, known as “OSeMOSYS,” with an add-on tool able to estimate different types of Levelized Cost Of Electricity (LCOE): a real and theoretical LCOE of each technology and a real and theoretical system LCOE. This tool fills a gap in most modeling frameworks characterized by a lack of information when evaluating energy costs and aims at guiding policymakers to the most appropriate solution. The model is then used to predict future energy scenarios for the island of San Pietro, in Sardinia, which was chosen as a case study. Four energy scenarios with a time horizon from 2020 to 2050—the Business-As-Usual (BAU) scenario, the Current Policy Projection (CPP) scenario, the Sustainable Growth (SG) scenario, and the Self-Sufficient-Renewable (SSR) scenario—are explored and ranked according to the efforts made in them to achieve an energy transition. Results demonstrates the validity of the tool, showing that, in the long run, the average LCOE of the system benefits from the installation of RES plants, passing from 49.1 €/MWh in 2050 in the BAU scenario to 48.8 €/MWh in the ambitious SG scenario. On the other hand, achieving carbon neutrality and the island’s energy independence brings the LCOE to 531.5 €/MWh, questioning the convenience of large storage infrastructures in San Pietro and opening up future work on the exploration of different storage systems.
Experimental and Mechanistic Study of Synergistic Removal of Hg by Evaporation from Desulfurization Wastewater
Authors: Hu Bin , Chen Cong , Yi Yang , Jiang Shouxi , Liu Xiaosong
Published: June 2022

The flue evaporation of desulfurization wastewater can solve the problem that it is difficult to remove some heavy metal ions and chloride ions by conventional methods. A large amount of chloride ions in desulfurization wastewater can also promote the catalytic oxidation removal of Hg in the flue gas. The migration character of chloride ions in the flue evaporation process of desulfurization wastewater was studied by using the coal-fired thermal state experimental platform. The concentrations of Hg0 and Hg2+ in the flue gas at the inlet and outlet of selective catalytic reduction denitration (SCR), electrostatic precipitator (ESP), and wet desulfurization (WFGD) devices were tested, and the synergistic removal of traditional pollutant removal equipment by flue evaporation of desulfurization wastewater was analyzed. The influence of Hg and the effect of the evaporation of desulfurization wastewater at different positions on the removal of Hg in the flue gas were compared and analyzed, and the catalytic mechanism of Hg on the SCR surface was further revealed. The results show that 10% chloride ions enter the flue gas after the desulfurization wastewater evaporates. The content of chlorine elements and evaporation temperature influence the evaporation of desulfurization wastewater. The mechanism of SCR catalytic oxidation of Hg0 was explored; oxygen atoms have catalytic oxidation effects on Hg0 at different positions in the V2O5 molecule in SCR; and chloride ions can enhance the catalytic oxidation of Hg0 by V2O5. The intermediate product HgCl is generated, which is finally converted into HgCl2. The oxidation efficiency of Hg0 in electrostatic precipitation (ESP) is increased from 3% to 18%, and the removal efficiency of Hg is increased from 5% to 10%. The removal efficiency of Hg2+ in WFGD is basically maintained at approximately 85%. In addition, a small amount of Hg2+ was restored to Hg0 in WFGD. The removal efficiency of Hg0 in the flue gas of evaporative desulfurization wastewater before SCR is 65%, and the removal efficiency of gaseous Hg is 62%. When the evaporative desulfurization wastewater before ESP, the synergistic removal efficiency of Hg0 is 39%, and the gaseous Hg removal efficiency is 39%, and the removal efficiency of Hg is 40%. Evaporation of the desulfurization wastewater before SCR was more conducive to the coordinated removal of Hg by the device.
Digital Twin Development of a Dynamic Hardware Emulator
Authors: Kellermüller Sandro , Obushevs Artjoms , Ramirez Gonzalez Miguel , Korba Petr
Published: June 2022

The increasing deployment of new technologies to contribute to the decarbonization of power systems is imposing new challenges in terms of system dynamics and stability. To deal with different operating and control issues in this sense, and support actual needs, advanced tools and solutions are required. Therefore, this paper presents a digital twin of a dynamic hardware emulator that can be used for controller hardware in the loop (CHIL) testing and is based on a small-scale laboratory system. To build the simulation model, the parameters of involved synchronous machines, excitation systems, prime movers and transmission lines have been identified and then compared to laboratory measurements to assess the accuracy of the digital twin. Static and dynamic accuracy have been investigated and an overall good accuracy can be shown with the help of quantification of errors. Furthermore, a case study is presented where the digital twin was used to design a controller to damp inter-area oscillations with the help of wide area measurements. This controller was then implemented and tested within the dynamic hardware emulator in the laboratory.
Stochastic Simulation of Flow Rate and Power Consumption Considering the Uncertainty of Pipeline Cracking Rate and Time-Dependent Topology of a Natural Gas Transmission Network
Authors: Alzbutas Robertas , Iešmantas Tomas
Published: June 2022

Various gas pipeline networks used for the transit of energy sources are some of the most important infrastructures. However, carrying gas from one point to another is not the only concern when planning the construction of a new network or expanding an already existing one. The reliability and environmental impact of the system are crucial when evaluating the network and risks posed by potential gas leaks, fires, explosions, etc. Even though everyone admits that reliability is a key aspect of any system, its constraints will still be most likely neglected in the assessment of the pipeline project. How much energy is wasted by pushing an additional amount of gas through the pipeline network, which will eventually gush out of the pipeline because of one crack or another? Moreover, if this additional power or fuel consumption and related environmental impact are significant, how could it be reduced? In this paper, an approach is introduced for the simulation and quantification of how much more power would be required if the pipelines are regarded as unreliable (i.e., by leaking, rupturing, or even exploding). By employing stochastic simulations and time-dependent topology (topology determined by the value of a variable representing time) of the pipeline network as a case study for the selected representative gas transmission network, the amount of additional power consumption in gas compressor stations due to uncertain cracking and the leaking rate was evaluated. Although the analysis of power consumption was performed for a hypothetical network, the estimates of the cracking rates, detection effectiveness, and leaking rates used were as close to the real cases as possible.
Can the Life History Trait Divergence of Two Extremes of a Cold-Water Genus Distribution Offer Evidence for Their Vulnerability to Sea Warming?
Authors: Serrat Alba , Muñoz Marta
Published: June 2022

Cold- and deep-water species such as Molva species show low resilience to anthropogenic pressures, and they become particularly vulnerable at the warm edges of their distribution. In this study, the poorly documented Mediterranean ling (Molva macrophthalma) population from the northwestern Mediterranean Sea was analysed. This area is considered a cul-de-sac in a sea-warming hotspot, where M. macrophthalma shows a low population health status and is experiencing a climate-related reduction in abundance. Several life-history traits (length at maturity, reproductive cycle, fecundity style, oocyte recruitment pattern, and breeding strategy) are here described for the first time to evaluate the reproductive performance (oocyte diameter and production) in relation to the fish condition status (the HSI and relative condition index). Additionally, the results are compared with those of a population of a similar species, the blue ling (Molva dypterygia), inhabiting the cool edge of its distribution, hypothesised to have a higher condition status. Our results indicate that M. macrophthalma is a capital breeder with restricted secondary growth recruitment and group-synchronous oocyte development. In relative terms, the stressed southern M. macrophthalma exhibited a worse condition, a lower investment in reproduction, a smaller size at maturity, larger but fewer primary growth oocytes, and a smaller size-standardized production of secondary growth oocytes than the northern M. dypterygia. Significant differences in the secondary growth oocyte recruitment were also found. These findings reinforce the environment’s role in shaping the reproductive potential and condition status. Altogether, this study suggests high sensitivity to anthropogenic pressures for both species, and, especially for Mediterranean ling, it shows the importance of introducing monitoring and conservation measures to ensure the sustainability of its populations.
Enzyme Activity and Physiochemical Properties of Flour after Supercritical Carbon Dioxide Processing
Authors: Leitgeb Maja , Knez Željko , Hojnik Podrepšek Gordana
Published: June 2022

The objectives of this study were to inactivate the enzymes α-amylase, lipase, protease, and peroxidase in flour with supercritical carbon dioxide (scCO2), and to optimize the enzymatic treatment conditions. Enzyme inactivation is important, due to the undesirability of certain flour enzymes that cause adverse reactions during storage as unpleasant rancidity of flour, and, at the same time, reduce the shelf life of flour. Therefore, crude enzymes and flour were initially exposed to scCO2 to determine the effect on specific enzyme activity under appropriate conditions. The activity of the unwanted enzymes lipase and peroxidase decreased under optimal process conditions of scCO2 exposure, lipase by 30%, and peroxidase by 12%, respectively. It was discovered that the inactivation of enzymes in wheat flour occurred, where, at the same time, this sustainable method allows the regulation of enzyme activity in the baking process. Afterwards, the effect of scCO2 on the physicochemical properties of flour, morphological changes on starch granules, and content of total lipids was studied. In scCO2-treated white wheat flour, the fat content decreased by 46.15 ± 0.5%, the grain structure was not damaged, and the bread as the final product had a lower specific surface volume. Therefore, this could be a promising technology for flour pretreatment, potentially impacting the prolonging of its shelf-life.
Registered Drug Packs of Antimicrobials and Treatment Guidelines for Prostatitis: Are They in Accordance?
Authors: Jerkovic Ivan , Seselja Perisin Ana , Bukic Josipa , Leskur Dario , Bozic Josko , Modun Darko , Vukovic Jonatan , Rusic Doris
Published: June 2022

The aim of this study was to analyze if registered drug packs of antibiotics are in accordance with national guidelines for prostatitis treatment regard to the amount of drug units.; Methods: Croatian, UK (NICE), Australian, Spanish and Slovenian national guidelines were analyzed in this study. Results: Comparing treatment guidelines with registered drug packs resulted in perfect accordance only for drug packs registered in the UK with the NICE guidelines, where even split-pack dispensing is possible. Interestingly, when comparing drug packs registered in the UK with treatment proposed in the national guidelines of Croatia, Italy, Spain, Australia, USA and Slovenia, they matched almost perfectly. In other investigated countries, registered drug packs’ national guidelines’ analysis showed mismatch in 25–100% of recommendations (Italy and Slovenia, respectively). Conclusions: Mismatch between registered drug packs that are dispensed to patients and treatment guidelines may result in excess units of antimicrobials that may be misused by the patient in the future, or excess antimicrobials may become unnecessary waste, further promoting antimicrobial resistance. Greater accordance of registered drug packs of antimicrobials with treatment guidelines may lower rates of antimicrobials misuse.
Oxidative Damage to Mitochondria Enhanced by Ionising Radiation and Gold Nanoparticles in Cancer Cells
Authors: Tabatabaie Farnaz , Franich Rick , Feltis Bryce , Geso Moshi
Published: June 2022

Gold nanoparticles (AuNP) can increase the efficacy of radiation therapy by sensitising tumor cells to radiation damage. When used in combination with radiation, AuNPs enhance the rate of cell killing; hence, they may be of great value in radiotherapy. This study assessed the effects of radiation and AuNPs on mitochondrial reactive oxygen species (ROS) generation in cancer cells as an adjunct therapeutic target in addition to the DNA of the cell. Mitochondria are considered one of the primary sources of cellular ROS. High levels of ROS can result in an intracellular state of oxidative stress, leading to permanent cell damage. In this study, human melanoma and prostate cancer cell lines, with and without AuNPs, were irradiated with 6-Megavolt X-rays at doses of 0–8 Gy. Indicators of mitochondrial stress were quantified using two techniques, and were found to be significantly increased by the inclusion of AuNPs in both cell lines. Radiobiological damage to mitochondria was quantified via increased ROS activity. The ROS production by mitochondria in cells was enhanced by the inclusion of AuNPs, peaking at ~4 Gy and then decreasing at higher doses. This increased mitochondrial stress may lead to more effectively kill of AuNP-treated cells, further enhancing the applicability of functionally-guided nanoparticles.
The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean
Authors: Yang Yunhua , Karthikeyan Adhimoolam , Yin Jinlong , Jin Tongtong , Ren Rui , Fang Fei , Cai Han , Liu Mengzhuo , Wang Dagang , Li Kai , Zhi Haijian
Published: June 2022

E3-ubiquitin ligases are known to confer abiotic stress responses in plants. In the present study, GmPUB21, a novel U-box E3-ubiquitin ligase-encoding gene, was isolated from soybean and functionally characterized. The expression of GmPUB21, which possesses E3-ubiquitin ligase activity, was found to be significantly up-regulated by drought, salinity, and ABA treatments. The fusion protein GmPUB21-GFP was localized in the cytoplasm, nucleus, and plasma membrane. Transgenic lines of the Nicotiana benthamiana over-expressing GmPUB21 showed more sensitive to osmotic, salinity stress and ABA in seed germination and inhibited mannitol/NaCl-mediated stomatal closure. Moreover, higher reactive oxygen species accumulation was observed in GmPUB21 overexpressing plants after drought and salinity treatment than in wild-type (WT) plants. Contrarily, silencing of GmPUB21 in soybean plants significantly enhanced the tolerance to drought and salinity stresses. Collectively, our results revealed that GmPUB21 negatively regulates the drought and salinity tolerance by increasing the stomatal density and aperture via the ABA signaling pathway. These findings improved our understanding of the role of GmPUB21 under drought and salinity stresses in soybean.
Genome-Wide Analysis of the Peptidase M24 Superfamily in Triticum aestivum Demonstrates That TaM24-9 Is Involved in Abiotic Stress Response
Authors: Yan Lu-Yu , Guo Jia-Gui , Zhang Xin , Liu Yang , Xiong Xin-Xin , Han Yu-Xuan , Zhang Li-Li , Zhang Xiao-Hong , Min Dong-Hong
Published: June 2022

The peptidase M24 (Metallopeptidase 24, M24) superfamily is essential for plant growth, stress response, and pathogen defense. At present, there are few systematic reports on the identification and classification of members of the peptidase M24 proteins superfamily in wheat. In this work, we identified 53 putative candidate TaM24 genes. According to the protein sequences characteristics, these members can be roughly divided into three subfamilies: I, II, III. Most TaM24 genes are complex with multiple exons, and the motifs are relatively conserved in each sub-group. Through chromosome mapping analysis, we found that the 53 genes were unevenly distributed on 19 wheat chromosomes (except 3A and 3D), of which 68% were in triads. Analysis of gene duplication events showed that 62% of TaM24 genes in wheat came from fragment duplication events, and there were no tandem duplication events to amplify genes. Analysis of the promoter sequences of TaM24 genes revealed that cis-acting elements were rich in response elements to drought, osmotic stress, ABA, and MeJA. We also studied the expression of TaM24 in wheat tissues at developmental stages and abiotic stress. Then we selected TaM24-9 as the target for further analysis. The results showed that TaM24-9 genes strengthened the drought and salt tolerance of plants. Overall, our analysis showed that members of the peptidase M24 genes may participate in the abiotic stress response and provided potential gene resources for improving wheat resistance.
Climatic Change of Summer Wind Direction and Its Impact on Hydrodynamic Circulation in the Pearl River Estuary
Authors: Hong Bo , Xue Hanlu , Zhu Liangsheng , Xu Hongzhou
Published: June 2022

Assessing the trend of sea surface wind is important for understanding the response of the marine environment to climate change. Analysis of wind data reveals that the summer wind direction in the Pearl River Estuary (PRE) shifts anticlockwise at a rate of −0.36°yr−1 over the past 42 years (1979–2020). The mean wind direction in July shifts from 183.6° (in 1979) to 169.3° (in 2020) and is predicted as 142.1° by 2100. How this long-term wind direction change affects the PRE hydrodynamic circulation structure has not been examined yet. A fully calibrated high resolution 3D hydrodynamic model is used to evaluate the response of local hydrodynamics to wind direction shifting in this study. The model results indicate that both the cross-channel wind-driven transport and along-channel seaward flow are weakened as wind direction shifts. Consequently, the lateral circulation is slowed down significantly while the longitudinal exchange flow is weakened slightly. A remarkable increase in stratification occurs in the coastal sea adjacent to the Modaomen where hypoxia has been frequently reported. The residence time of Lingding Bay increases slightly. The Momentum budget indicates the wind direction shifting can cause major changes in the barotropic pressure term, which is mainly balanced by the baroclinic pressure term and diffusion term.
New Species of Talaromyces (Trichocomaceae, Eurotiales) from Southwestern China
Authors: Wang Xin-Cun , Zhuang Wen-Ying
Published: June 2022

Species of Talaromyces are cosmopolitan and ubiquitous, and some are of industrial and medicinal importance. Species of Talaromyces have been successively reported in China. During our examinations of samples collected from southwestern China, two new species belonging to Talaromyces sect. Talaromyces were further discovered based on phylogenetic analyses and morphological comparisons. Talaromyces ginkgonis sp. nov., isolated from a partially colonized fruit of Ginkgo biloba, differs from closely-related fungi in the combination of conidia ellipsoidal, smooth and 3.5−4 × 2−3 μm, no growth on CYA at 37 °C and sequence divergences; T. shilinensis sp. nov. is distinguished from its related allies in the combination of smooth conidia, colonies 10−11 mm diameter on CYA at 25 °C and sequence differences. Detailed descriptions and illustrations of the new taxa are given.
Critical Language Awareness in the Spanish as a Heritage Language College Classroom
Authors: Hudgens Henderson Mary
Published: June 2022

This article analyzes the critical language awareness (CLA) of Spanish university-level students who were enrolled in a 16-week Spanish heritage language (SHL) course, using CLA as an instructional approach. Students’ attitudes towards bilingualism, Spanglish, language variation, and prescriptivist grammar were measured via pretest and posttest surveys that used a four-point Likert scale of strongly agree, agree, disagree, or strongly disagree, along with a text box asking participants to explain their answers. The CLA instructional methods delivered in the course included the analysis of code-switching grammar constraints, the study of standard language ideologies and monolingual language ideologies, the analysis of stigmatized grammar features found in varieties of Spanish, and English-influenced lexicon. Ten out of fourteen items were included in a factor analysis which yielded a statistically significant change between pretest and posttest. Qualitative analysis of answer explanations showed that some students adapted their language ideologies to the new information and did not change their beliefs at a deep level. Future CLA research should identify “CLA proficiency” levels as well as how to differentiate for students who hold deeply entrenched language ideologies.
Development of Flexible Fixtures with Incomplete Locating: Connecting Rods Machining Case Study
Authors: Ivanov Vitalii , Botko František , Dehtiarov Ivan , Kočiško Marek , Evtuhov Artem , Pavlenko Ivan , Trojanowska Justyna
Published: June 2022

The rapid development of manufacturing in recent years has led to a significant expansion of the technological capabilities of modern metal-cutting equipment. Therefore, the modern approach to intensifying production requires an advanced fixture design. Design and manufacture of flexible fixtures capable of machining similar shapes and sizes of complex geometry parts reduce setup time. The article aims to design flexible fixtures for parts such as one-piece connecting rods under incomplete locating conditions. The advantages are the minimum number of parts and tool availability for multi-axis machining connecting rods in one setup. This approach, combined with up-to-date machining centers and industrial robots, can increase the production efficiency of manufacturing non-removable connecting rods. This effectiveness is in a decrease in the number of operations by 5–7 times, fixtures—by 3–4 times, and machine tools—by 3–5 times, depending on the type of a non-removable connecting rod and its design features. The numerical simulation results of the proposed fixture design confirmed the comprehensive technological capabilities and dynamic characteristics. Particularly, a decrease in displacements and oscillation amplitudes up to 7% compared to the full-basing locating chart was provided. It is determined that the system “fixture–workpiece” entirely meets all the strength, accuracy, and rigidity parameters, which allows you to perform machining with intensive cutting modes. The amplitudes of oscillations do not exceed the tolerances on the dimensions of these surfaces, established by requirements for non-removable connecting rods, and all displacements are elastic. During numerical simulation, the workpiece position remained stable at all machining steps.
Estimation of the Fractal Dimensions of the Linear Combination of Continuous Functions
Authors: Yu Binyan , Liang Yongshun
Published: June 2022

In the present paper, we try to estimate the fractal dimensions of the linear combination of continuous functions with different fractal dimensions. Initially, a general method to calculate the lower and the upper Box dimension of the sum of two continuous functions by classifying all the subsequences into different sets has been proposed. Further, we discuss the majority of possible cases of the sum of two continuous functions with different fractal dimensions and obtain their corresponding fractal dimensions estimation by using that general method. We prove that the linear combination of continuous functions having no Box dimension cannot keep the fractal dimensions closed. In this way, we have figured out how the fractal dimensions of the linear combination of continuous functions change with certain fractal dimensions.
Adopting Nonlinear Activated Beetle Antennae Search Algorithm for Fraud Detection of Public Trading Companies: A Computational Finance Approach
Authors: Liao Bolin , Huang Zhendai , Cao Xinwei , Li Jianfeng
Published: June 2022

With the emergence of various online trading technologies, fraudulent cases begin to occur frequently. The problem of fraud in public trading companies is a hot topic in financial field. This paper proposes a fraud detection model for public trading companies using datasets collected from SEC’s Accounting and Auditing Enforcement Releases (AAERs). At the same time, this computational finance model is solved with a nonlinear activated Beetle Antennae Search (NABAS) algorithm, which is a variant of the meta-heuristic optimization algorithm named Beetle Antennae Search (BAS) algorithm. Firstly, the fraud detection model is transformed into an optimization problem of minimizing loss function and using the NABAS algorithm to find the optimal solution. NABAS has only one search particle and explores the space under a given gradient estimation until it is less than an “Activated Threshold” and the algorithm is efficient in computation. Then, the random under-sampling with AdaBoost (RUSBoost) algorithm is employed to comprehensively evaluate the performance of NABAS. In addition, to reflect the superiority of NABAS in the fraud detection problem, it is compared with some popular methods in recent years, such as the logistic regression model and Support Vector Machine with Financial Kernel (SVM-FK) algorithm. Finally, the experimental results show that the NABAS algorithm has higher accuracy and efficiency than other methods in the fraud detection of public datasets.
Study on Failure Characteristics and Control Technology of Roadway Surrounding Rock under Repeated Mining in Close-Distance Coal Seam
Authors: Shang Yuqi , Kong Dezhong , Pu Shijiang , Xiong Yu , Li Qiang , Cheng Zhanbo
Published: June 2022

In this study, taking the Sheng’an coal mine as an engineering background, the failure characteristics of the surrounding rock of a roadway under repeated mining in a close-distance coal seam is comprehensively illustrated through field measurements (e.g., drilling imaging), theory analysis and numerical simulation (finite difference method (FDM)). The results show that although the return airway 10905 remains intact, the apparent failure of the roadway’s roof and the coal pillar can be observed. In addition, the expression of floor failure depth caused by upper coal seam mining is obtained through elastic-plastic theory. Meanwhile, the deformation of the surrounding rock of the roadway increases with the increase of repeated mining times, especially for the horizontal displacement of the roadway on the coal pillar side. Moreover, the cracks’ evolution of surrounding rock in the roadway can be observed as asymmetric characteristics. Finally, the stability control technology of “asymmetric anchor net cable + I-steel” is proposed to prevent potential mining disasters, and the feasibility of this support scheme is verified by numerical simulation and field practices. It can meet the requirement of safe mining and provide guidelines to effectively solve the failure of a roadway in close-distance coal seam mining.
Oxidative Stress in Chronic Hepatitis B—An Update
Authors: Popa Gabriela Loredana , Popa Mircea Ioan
Published: June 2022

In recent years, the role of oxidative stress has been investigated in an increasing number of infections. There is a close link between the inflammation that accompanies infections and oxidative stress. Excessive reactive oxygen species induce harmful effects on cell components, including lipids, proteins, and nucleic acids. A growing body of evidence attests to the role of oxidative stress in the pathogenesis of viral liver infections, especially in hepatitis C virus (HCV) infection. Regarding hepatitis B virus (HBV) infection, the data are limited, but important progress has been achieved in recent years. This review presents the latest advances pertaining to the role of the oxidative stress byproducts in the pathogenesis of chronic hepatitis B, constituting a source of potential new markers for the evaluation and monitoring of patients with chronic hepatitis B.
Roles of Arbuscular mycorrhizal Fungi as a Biocontrol Agent in the Control of Plant Diseases
Authors: Weng Wenfeng , Yan Jun , Zhou Meiliang , Yao Xin , Gao Aning , Ma Chao , Cheng Jianping , Ruan Jingjun
Published: June 2022

Arbuscularmycorrhizal fungi (AMF) are a class of beneficial microorganisms that are widely distributed in soil ecosystems and can form symbionts with 80% of terrestrial higher plants, and improve the nutritional status of plants. The use of AMF as a biocontrol method to antagonize soil-borne pathogens has received increasing interest from phytopathologists and ecologists. In this paper, the mechanisms of resistance to diseases induced by AMF and the application of AMF to plant fungal, bacterial, and nematode diseases have been summarized. This study aimed to enhance the potential use of AMF as a biological control method to prevent plant diseases in the future. Root morphological alteration characteristics were explained, including the influence of AMF on root structure, function, and the regulation of AMF via secondary metabolites. AMF can improve the rhizosphere environment by influencing the physical and chemical proprieties of soil, enhancing the growth of other beneficial microorganisms, and by competing with pathogenic microorganisms. Two microorganism types may compete for the same invasive sites in root systems and regulate nutrition distribution. AMF can induce the host plant to form defense systems, including improving phytohormone concentrations, inducing signal substrate production, gene expression regulation, and enhancing protein production.
Exploration of the Crystal Structure and Thermal and Spectroscopic Properties of Monoclinic Praseodymium Sulfate Pr2(SO4)3
Authors: Denisenko Yuriy G. , Atuchin Victor V. , Molokeev Maxim S. , Sedykh Alexander E. , Khritokhin Nikolay A. , Aleksandrovsky Aleksandr S. , Oreshonkov Aleksandr S. , Shestakov Nikolai P. , Adichtchev Sergey V. , Pugachev Alexey M. , Sal’nikova Elena I. , Andreev Oleg V. , Razumkova Illaria A. , Müller-Buschbaum Klaus
Published: June 2022

Praseodymium sulfate was obtained by the precipitation method and the crystal structure was determined by Rietveld analysis. Pr2(SO4)3 is crystallized in the monoclinic structure, space group C2/c, with cell parameters a = 21.6052 (4), b = 6.7237 (1) and c = 6.9777 (1) Å, β = 107.9148 (7)°, Z = 4, V = 964.48 (3) Å3 (T = 150 °C). The thermal expansion of Pr2(SO4)3 is strongly anisotropic. As was obtained by XRD measurements, all cell parameters are increased on heating. However, due to a strong increase of the monoclinic angle β, there is a direction of negative thermal expansion. In the argon atmosphere, Pr2(SO4)3 is stable in the temperature range of T = 30–870 °C. The kinetics of the thermal decomposition process of praseodymium sulfate octahydrate Pr2(SO4)3·8H2O was studied as well. The vibrational properties of Pr2(SO4)3 were examined by Raman and Fourier-transform infrared absorption spectroscopy methods. The band gap structure of Pr2(SO4)3 was evaluated by ab initio calculations, and it was found that the valence band top is dominated by the p electrons of oxygen ions, while the conduction band bottom is formed by the d electrons of Pr3+ ions. The exact position of ZPL is determined via PL and PLE spectra at 77 K to be at 481 nm, and that enabled a correct assignment of luminescent bands. The maximum luminescent band in Pr2(SO4)3 belongs to the 3P0 → 3F2 transition at 640 nm.