bluuebunny/crossref_metadata_2025 huggingface dataset:
Machine Learning for Early Prediction of Sepsis in Intensive Care Unit (ICU) Patients
Authors: Alanazi Abdullah , Aldakhil Lujain , Aldhoayan Mohammed , Aldosari Bakheet
Published: July 2023

Background and Objectives: Early detection of sepsis is crucial and can save lives. However, identifying sepsis early and accurately remains a difficult task in the medical field. This study aims to investigate a new machine-learning approach. By analyzing the clinical laboratory results and vital signs of adult patients in the ICU, this approach can predict and detect the initial signs of sepsis. Materials and Methods: To examine survival rates and predict outcomes, the study utilized several models, including the proportional hazards model and data mining algorithms. We analyzed data from the BESTCare database at KAMC, with a focus on patients aged 14 and older who were admitted to the ICU between April and October 2018. We conducted a thorough analysis of the medical records of a total of 1182 patients who were diagnosed with sepsis. Results: We studied two approaches to predict sepsis in ICU patients. The regression model utilizing survival analysis showed moderate predictive ability, emphasizing the importance of only three factors—time (from sepsis to an outcome; discharge or death), lactic acid, and temperature—had a significant p-value (p = 0.000568, p = 0.01, p = 0.02, respectively). Other data mining algorithms may have limitations due to their assumptions of variable independence and linear classification nature. Conclusions: To achieve progress and accuracy in the field of sepsis prediction, it is important to continuously strive for improvement. By meticulously cleaning and selecting data attributes, we can create a strong foundation for future advancements in this area.
Effect of Mg-Ce Treatment on Inclusion Characteristics and Pitting Corrosion Behavior in EH420 Marine Steel
Authors: Guo Zhihong , Liu Erkang , Wang Qi , Lou Xiangjie , Liu Hongbo , Zheng Yaxu , Wang Bo , Zhu Liguang
Published: July 2023

Reasonable regulation of nonmetallic inclusions in steel can significantly improve its strength, toughness, and corrosion resistance. In this paper, EH420 marine steel was treated with Mg, Ce, and Mg-Ce to modify the inclusions. The effects of different treatments on the morphology, composition, size distribution, induced intragranular ferrite (IGF) nucleation, and pitting resistance of inclusions were systematically analyzed using various methods. The results show that the Mg-Ce composite treatment can modify irregular MgAl2O4 inclusions into spherical Mg-Ce-O composite inclusions and MgO-dominated inclusions. The density of inclusions is increased from 74.8/mm2 to 186.0/mm2, and the average size of inclusions is decreased from 2.60 μm to 1.07 μm. The Mg-Ce-O composite inclusions are effective inclusions for inducing IGF. Furthermore, the pitting potential is increased from −503 mV to −487 mV, and the corrosion rate is decreased. The order of average electronic work function is ΦMgO < ΦCe2O3 < Φα-Fe < ΦAl2O3. Ce2O3 is hard to induce pitting corrosion due to its similar electronic work function to the steel matrix. Thus, the Mg-Ce composite treatment is better than that for Mg and Ce treatment alone, and has better application prospects.
Multi-Scale Structuring of CoCrMo and AZ91D Magnesium Alloys Using Direct Laser Interference Patterning
Authors: Henriques Bruno , Fabris Douglas , Voisiat Bogdan , Lasagni Andrés Fabián
Published: July 2023

In this work, the technique of Direct Laser Interference Patterning (DLIP) was used to fabricate micrometric structures at the surface of Cobalt-Chromium-Molybdenum and AZ91D magnesium alloys. Line-like patterns with spatial periods of 5 μm were textured using an ultra-short pulsed laser (10 ps pulse duration and 1064 nm wavelength) with a two-beam interference setup. The surface topography, morphology, and chemical modifications were analysed using Confocal Microscopy, Scanning Electron Microscopy, and Energy Dispersive Spectroscopy (EDS), respectively. Laser fluence and pulse overlap were varied to evaluate their influence on the final structure. Homogeneous structures were achieved for the CoCrMo alloy for every condition tested, with deeper structures (up to 0.85 μm) being achieved for higher energy levels (higher overlap and/or fluence). For high energy, sub-micrometric secondary structures, so-called LIPSS, could also be observed on the CoCrMo. The EDS analysis showed some oxidation after the laser texturing. Regarding the AZ91D alloy, deeper structures could be achieved (up to 2.5 μm), but more melting and oxidation was observed, forming spherical oxide particles. Nonetheless, these results bring new perspectives on the fabrication of microtextures on the surface of CoCrMo and AZ91D using DLIP.
Leaching Kinetics of Hemimorphite with 5-Sulfosalicylic Acid
Authors: Li Yaohong , Wen Shuming , Cao Jing , Wu Dandan , Wang Yijie
Published: July 2023

The kinetics of leaching zinc from hemimorphite was investigated. The factors that influence hemimorphite leaching were also evaluated, and a kinetic model was built. In addition, scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) was used to investigate the changes of surface morphology before and after leaching. By decreasing particle size and increasing temperature, 5-sulfosalicylic acid concentration, and stirring speed, the leaching rate of hemimorphite can be enhanced. The shrinkage nucleus model describes the surface chemistry of leaching. The activation energy of hemimorphite by 5-sulfosalicylic acid in the leaching process was determined as 55.244 kJ/mol. The reaction rate based on the shrinkage nucleus model can be expressed by the semi-empirical formula:1−1−x1/3 =[k0C0.3385(r0)−0.6083(SS)0.4992exp(−55.244/RT)]t. At the condition of 50 °C of leaching temperature, 0.175 mol/L of 5-sulfosalicylic acid concentration, 82.5 μm of particle size and 650 rpm of stirring speed, the high leaching rates of zinc were obtained. After the reaction time of 15 min, the leaching rate of zinc reached more than 95%. According to the SEM-EDS results, the hemimorphite and leaching residue are distributed in blocks, but the particle size of the leaching residue is smaller, and the atomic concentrations of Zn and Si in the leaching residue are significantly lower than those in the hemimorphite, so the leaching effect is remarkable. Therefore, 5-sulfosalicylic acid solution would be an excellent leaching agent for zinc extraction from hemimorphite.
Identification, Biological Function Profiling and Biosynthesis of Secondary Metabolites in Medicinal Orchids
Authors: Li Kunqian , Wu Fengju , Chen Mengzhu , Xiao Zhihao , Xu Ya , Xu Mengwei , Liu Jingyi , Xu Delin
Published: July 2023

The secondary metabolites present in medicinal orchids are diverse and possess a vast array of biological activities. They represent valuable raw materials for modern pharmaceuticals and clinical medicine and have tremendous potential for future development. A systematic collation of secondary metabolites’ composition and a summary of the biological activities of medicinal orchids represent a crucial step in unlocking the potential of these valuable resources in drug development. Furthermore, such information can provide essential guidance for comprehensively analyzing the pharmacological and therapeutic mechanisms of these valuable herbs in traditional Chinese herbal medicine. This review article presents an overview of the types and main biological functions of the secondary metabolites found in medicinal orchids, as well as the conventional synthesis methods for these compounds. Our aim is to provide a useful reference for future research and the drug development of secondary metabolic products of medicinal orchids.
Hetero-Pathogenic O181:H4 EAHEC Strain of Sequence Type ST678 Associated with Hemolytic–Uremic Syndrome in Schoolchildren in Russia
Authors: Kartsev Nikolay N. , Detusheva Elena V. , Kalmantaeva Olga V. , Korobova Olga V. , Gerasimov Vladimir N. , Kombarova Tatiana I. , Borzilov Aleksander I. , Fursova Nadezhda K. , Vereshchagin Anatoly N. , Svetoch Edward A.
Published: July 2023

Background: In the last decade, the importance of hetero-pathogenic enteroaggregative Shiga-toxin-producing E. coli for public health has increased. Recently, we described the genetic background of the EAHEC O181:H4 strain of ST678 carrying the stx2 gene in prophage and five plasmids, including the plasmid-carrying aggR and aaiC genes. Here, we present the morphological and enzymatic characteristics of this strain, as well as susceptibility to antimicrobials, biofilm formation, etc. Methods: Bacterial morphology was studied using an electron microscope. Susceptibility to antimicrobials was determined using the microdilution method. Cytotoxicity was estimated in Vero cells. Virulence was studied on mice. Results: The morphological and enzymatic properties of the hetero-pathogenic EAHEC strain were typical for E. coli; electron microscopy revealed the specific flagella. The strain was susceptible to most antibiotics and disinfectants but resistant to ampicillin and ciprofloxacin and showed a high degree of biofilm formation. Cytotoxicity towards Vero cells was estimated as 80%. Conclusions: The emergence of a new O181:H4 EAHEC strain poses a potential threat to humans because of the virulence potential that must be taken into account in the epidemiological analysis of outbreaks and sporadic cases of foodborne infections associated with hemolytic–uremic syndrome.
Desulfovibrio in the Gut: The Enemy within?
Authors: Singh Sudha B. , Carroll-Portillo Amanda , Lin Henry C.
Published: July 2023

Desulfovibrio (DSV) are sulfate-reducing bacteria (SRB) that are ubiquitously present in the environment and as resident commensal bacteria within the human gastrointestinal tract. Though they are minor residents of the healthy gut, DSV are opportunistic pathobionts that may overgrow in the setting of various intestinal and extra-intestinal diseases. An increasing number of studies have demonstrated a positive correlation between DSV overgrowth (bloom) and various human diseases. While the relationship between DSV bloom and disease pathology has not been clearly established, mounting evidence suggests a causal role for these bacteria in disease development. As DSV are the most predominant genera of SRB in the gut, this review summarizes current knowledge regarding the relationship between DSV and a variety of diseases. In this study, we also discuss the mechanisms by which these bacteria may contribute to disease pathology.
Minimal Inhibitory Concentrations of Thymol and Carvacrol: Toward a Unified Statistical Approach to Find Common Trends
Authors: Speranza Barbara , Bevilacqua Antonio , Campaniello Daniela , Altieri Clelia , Corbo Maria Rosaria , Sinigaglia Milena
Published: July 2023

Thymol and carvacrol are some of the most important and used components of Essential oils (EOs); they are widely studied, and there are much data available in the literature. Their Minimal Inhibitory Concentration (MIC) values found in the literature from 2005 to present were used to assess the bioactivity toward yeasts, molds, Gram-positive bacteria, and Gram-negative bacteria, as well as on some bacterial species/serotypes (Salmonella sp., Escherichia coli, E. coli O157:H7, lactic acid bacteria, Listeria monocytogenes, Staphylococcus aureus, S. epidermidis, etc.) to find possible common trends or differences between the two compounds and among the tested species. The results were quite interesting and pointed out that there is a common range for the MIC of thymol and carvacrol for some bacterial species (150–400 mg/L), with some exceptions to this generalized statement. In addition, the statistics pointed out that bacteria could experience homogeneous (S. epidermidis, E. coli O157:H7) or heterogeneous trends (for example, Salmonella sp.) depending on the existence of possible sub-species or different experimental set-ups. Moreover, this paper suggests that there are some drawbacks and issues that should be solved for the effective use of EOs, which are the strong variability among the microorganisms and the lack of standard protocols and reference strains.
Monkeypox: A Histopathological and Transmission Electron Microscopy Study
Authors: Moltrasio Chiara , Boggio Francesca Laura , Romagnuolo Maurizio , Cagliani Rachele , Sironi Manuela , Di Benedetto Alessandra , Marzano Angelo Valerio , Leone Biagio Eugenio , Vergani Barbara
Published: July 2023

The global outbreak of human monkeypox virus (hMPXV1) in 2022 highlighted the usefulness of dermatological manifestations for its diagnosis. Infection by the human monkeypox virus thus necessitated inclusion in the diagnostic repertoire of dermatopathology. To assess the histopathological and microscopical findings of cutaneous lesions related to hMPXV infection, we analyzed skin biopsies from patients with positive MPXV DNA polymerase chain reaction presenting with a typical course of hMPXV1 infection. The most prominent histopathological findings were ascribable to a pustular stage in which epidermal necrosis with areas of non-viable keratinocytes and a “shadow cell” appearance were evident; in some cases, the deep portion of the hair follicle and the acrosyringial epithelium were affected. The main cytopathic modifications included ballooning keratinocytes, followed by Guarnieri bodies and a ground glass appearance of the keratinocytes’ nuclei, together with a dense mixed inflammatory cell infiltrate with prominent neutrophil exocytosis. Transmission electron microscopy analysis demonstrated viral particle aggregates in the cytoplasm of keratinocytes, without any involvement of the nucleus. Interestingly, we also found the presence of viral particles in infected mesenchymal cells, although to a lesser extent than in epithelial cells. Through this study, we contributed to expanding the histological and microscopic knowledge of the human mpox virus, a key step to understanding current and potential future trends of the disease, as well as of other Orthopoxvirus infections.
Effect of Raw Materials on Temperature Development during Prereduction of Comilog and Nchwaning Manganese Ores
Authors: Larssen Trine A. , Tangstad Merete
Published: July 2023

The effect of raw materials on the temperature development and prereduction behavior of Comilog and Nchwaning manganese ores (3.33–4.00 mm or 11.20–15.00 mm) were investigated in a CO-CO2 atmosphere. Experiments were conducted at a heating rate of 6 °C/min from room temperature to 1000 °C where the ore was mixed with inert material (quartz) in various ratios. A similar heating program gave dissimilar temperature development for Comilog and Nchwaning ores due to differences in oxygen levels and reduction rates. The temperature development, and thus reduction rate, decreased with the increasing amount of inert material in the mix for Comilog ore in size 3.33–4.00 mm. The peak reaction rates observed during reduction were 2.4 wt%/min, 1.0 wt%/min, and 0.8 wt%/min for an ore:quartz weight ratio of 1:0, 1:1, and 1:3, respectively. For Comilog ore in size 11.20–15.00 mm, and Nchwaning ore in both sizes, the temperature development and reduction rates were slightly or insignificantly affected. It was suggested that the effect was low for reactions under a diffusion-controlled kinetic regime.
Simulation of Capacitorless DRAM Based on the Polycrystalline Silicon Nanotube Structure with Multiple Grain Boundaries
Authors: Park Jin , Lee Sang-Ho , Kang Ga-Eon , Heo Jun-Hyeok , Jeon So-Ra , Kim Min-Seok , Bae Seung-Ji , Hong Jeong-Woo , Jang Jae-won , Bae Jin-Hyuk , Lee Sin-Hyung , Kang In-Man
Published: July 2023

In this study, a capacitorless one-transistor dynamic random-access memory (1T-DRAM), based on polycrystalline silicon (poly-Si) nanotube structure with a grain boundary (GB), is designed and analyzed using technology computer-aided design (TCAD) simulation. In the proposed 1T-DRAM, the 1T-DRAM cell exhibited a sensing margin of 422 μA/μm and a retention time of 213 ms at T = 358 K with a single GB. To investigate the effect of random GBs, it was assumed that the number of GB is seven, and the memory characteristics depending on the location and number of GBs were analyzed. The memory performance rapidly degraded due to Shockley–Read–Hall recombination depending on the location and number of GBs. In the worst case, when the number of GB is 7, the mean of the sensing margin was 194 µA/µm, and the mean of the retention time was 50.4 ms. Compared to a single GB, the mean of the sensing margin and the retention time decreased by 59.7% and 77.4%, respectively.
Effect of Femtosecond Laser-Irradiated Titanium Plates on Enhanced Antibacterial Activity and Preservation of Bacteriophage Stability
Authors: Grase Liga , Onufrijevs Pavels , Rezevska Dace , Racenis Karlis , Skadins Ingus , Karosas Jonas , Gecys Paulius , Iesalnieks Mairis , Pludons Arturs , Kroica Juta , Raciukaitis Gediminas
Published: July 2023

Titanium (Ti) is widely recognized for its exceptional properties and compatibility with medical applications. In our study, we successfully formed laser-induced periodic surface structures (LIPSS) on Ti plates with a periodicity of 520–740 nm and a height range of 150–250 nm. To investigate the morphology and chemical composition of these surfaces, we employed various techniques, including field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. Additionally, we utilized a drop-shape analyzer to determine the wetting properties of the surfaces. To evaluate the antibacterial activity, we followed the ISO 22196:2011 standard, utilizing reference bacterial cultures of Gram-positive Staphylococcus aureus (ATCC 25923) and Gram-negative Escherichia coli (ATCC 25922). The results revealed enhanced antibacterial properties against Staphylococcus aureus by more than 99% and Escherichia coli by more than 80% in comparison with non-irradiated Ti. Furthermore, we conducted experiments using the Escherichia coli bacteriophage T4 (ATCC 11303-B4) and the bacterial host Escherichia coli (ATCC 11303) to investigate the impact of Ti plates on the stability of the bacteriophage. Overall, our findings highlight the potential of LIPSS on Ti plates for achieving enhanced antibacterial activity against common bacterial strains while maintaining the stability of bacteriophages.
Silica Nanoparticle-Infused Omniphobic Polyurethane Foam with Bacterial Anti-Adhesion and Antifouling Properties for Hygiene Purposes
Authors: Cho Dongik , Oh Jun Kyun
Published: July 2023

In this study, a method for preventing cross-infection through the surface coating treatment of polyurethane (PU) foam using functionalized silica nanoparticles was developed. Experimental results confirmed that the fabricated PU foam exhibited omniphobic characteristics, demonstrating strong resistance to both polar and nonpolar contaminants. Additionally, quantitative analysis using the pour plate method and direct counting with a scanning electron microscope determined that the treated material exhibited anti-adhesion properties against bacteria. The fabricated PU foam also demonstrated a high level of resistance to the absorption of liquids commonly found in medical facilities, including blood, 0.9% sodium chloride solution, and 50% glycerol. Mechanical durability and stability were verified through repeated compression tests and chemical leaching tests, respectively. The proposed coated PU foam is highly effective at preventing fouling from polar and nonpolar fluids as well as bacteria, making it well-suited for use in a range of fields requiring strict hygiene standards, including the medical, food, and environmental industries.
Total Bio-Based Material for Drug Delivery and Iron Chelation to Fight Cancer through Antimicrobial Activity
Authors: Patamia Vincenzo , Zagni Chiara , Fiorenza Roberto , Fuochi Virginia , Dattilo Sandro , Riccobene Paolo Maria , Furneri Pio Maria , Floresta Giuseppe , Rescifina Antonio
Published: July 2023

Bacterial involvement in cancer’s development, along with their impact on therapeutic interventions, has been increasingly recognized. This has prompted the development of novel strategies to disrupt essential biological processes in microbial cells. Among these approaches, metal-chelating agents have gained attention for their ability to hinder microbial metal metabolism and impede critical reactions. Nanotechnology has also contributed to the antibacterial field by offering various nanomaterials, including antimicrobial nanoparticles with potential therapeutic and drug-delivery applications. Halloysite nanotubes (HNTs) are naturally occurring tubular clay nanomaterials composed of aluminosilicate kaolin sheets rolled multiple times. The aluminum and siloxane groups on the surface of HNTs enable hydrogen bonding with biomaterials, making them versatile in various domains, such as environmental sciences, wastewater treatment, nanoelectronics, catalytic studies, and cosmetics. This study aimed to create an antibacterial material by combining the unique properties of halloysite nanotubes with the iron-chelating capability of kojic acid. A nucleophilic substitution reaction involving the hydroxyl groups on the nanotubes’ surface was employed to functionalize the material using kojic acid. The resulting material was characterized using infrared spectroscopy (IR), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM), and its iron-chelating ability was assessed. Furthermore, the potential for drug loading—specifically, with resveratrol and curcumin—was evaluated through ultraviolet (UV) analysis. The antibacterial assay was evaluated following CLSI guidelines. The results suggested that the HNTs–kojic acid formulation had great antibacterial activity against all tested pathogens. The outcome of this work yielded a novel bio-based material with dual functionality as a drug carrier and an antimicrobial agent. This innovative approach holds promise for addressing challenges related to bacterial infections, antibiotic resistance, and the development of advanced therapeutic interventions.
Simple and Rapid Colorimetric Detection of Canine Parainfluenza Virus 5 (Orthorubulavirus mammalis) Using a Reverse-Transcription Loop-Mediated Isothermal Amplification Assay
Authors: Kim Jong-Min , Kim Hye-Ryung , Baek Ji-Su , Kwon Oh-Kyu , Kang Hae-Eun , Shin Yeun-Kyung , Park Choi-Kyu
Published: July 2023

Despite its many advantages, a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay has yet to be developed for canine parainfluenza virus 5 (CPIV5). In this study, a visual RT-LAMP (vRT-LAMP) assay was developed for the rapid detection of CPIV5 in clinical samples. At a constant reaction temperature of 62 °C, the assay was completed within 40 min, and the results could be directly detected with the naked eye using a hydroxynaphthol blue (HNB) metal indicator without any additional detection apparatuses. The assay specifically amplified CPIV5 RNA with a limit of detection of 10 RNA copies/reaction, which was 10-fold more sensitive than the previously reported conventional reverse-transcription polymerase chain reaction (cRT-PCR) assay and was comparable to the previously reported real-time RT-PCR (qRT-PCR) assay. In a clinical evaluation using 267 nasopharyngeal swab samples collected from hospitalized dogs with respiratory symptoms, the CPIV5 detection rate using the vRT-LAMP assay was 5.24% (14/267), which was higher than that of the cRT-PCR assay (4.49%, 12/267) and consistent with that of the qRT-PCR assay, demonstrating 100% concordance with a kappa coefficient value (95% confidence interval) of 1 (1.00–1.00). The discrepancies in the results of the assays were confirmed to be attributed to the low sensitivity of the cRT-PCR assay. Owing to the advantages of a high specificity, rapidity, and simplicity, the developed vRT-LAMP assay using an HNB metal indicator will be a valuable diagnostic tool for the detection of CPIV5 in canine clinical samples, even in resource-limited laboratories.
Discovery of a Digenean (Cryptogonimidae) Living in a Cleft-Lipped Goby, Sicyopterus cynocephalus (Teleostei: Gobiidae) from Ranongga Island, Solomon Islands: Analysis of Multiple Ribosomal DNA Regions
Authors: Mathews Patrick D. , Rabet Nicolas , L. Espinoza Luis , Haÿ Vincent , Bonillo Céline , Keith Philippe , Lord Clara , Audebert Fabienne
Published: July 2023

This study results from a continued investigation of the occurrence and diversity of parasites of freshwater fish in the Solomon Islands. Thus, we revealed a new host as well as a new site of infection and a new geographical area for the cryptogonimid parasite, Stemmatostoma cribbi (Digenea: Cryptogonimidae). The cryptogonimid species was identified based on general morphology and on molecular data of metacercariae found in the gills of the cleft-lipped goby, Sicyopterus cynocephalus, from Ranongga Island, Western Province of the Solomon Islands. This is the first report of a Stemmatostoma sp. digenean parasitizing fish of the genus Sicyopterus in the Indo-Pacific region and the first report of S. cribbi infection in a fish from the Solomon Islands. Phylogenetic analysis performed by Bayesian inference and maximum likelihood confirmed the presence of the cryptogonimid in a well-supported subclade of Stemmatostoma spp.
Spatial Spectral Characteristics of Partial Discharge with Different Electrode Models
Authors: Wang Taiqi , Cheng Yongkang , Xu Chao , Li Haoyu , Cheng Jiayao , Peng Gangding , Guo Qiang
Published: July 2023

In this paper, the spatial spectral characteristics of partial discharge (PD) under different electrode models are mainly studied. In the initial corona discharge stage, the emission spectrum is mainly emitted by the N2(C3IIu→B3IIg) energy level transition of the N2 second positive band system. The spectrum is in the ultraviolet range of 294–436 nm, and its main peak is at 337 nm. The streamer discharge stage spectrum is mainly emitted by the energy level transition of the second positive band system of N2, N+, NO, and O+ and the first positive band system of N2(B3IIg→A3Σu+). In the gap of different polarity electrodes, the ultraviolet spectrum content near the positive polarity is more abundant. The UV spectra ranges are 202–225 nm and 229–292 nm, respectively. The discharge of the needle–sphere system is more intense in visible light and near-infrared light, with peaks at 500 nm and 777 nm, respectively. In addition, the PD process based on the finite element method is simulated by COMSOL Multiphysics software. The simulation results show that the distribution of high-energy electron density varies with the electrode spacing and discharge model. The influence of particle energy level transition on the spatial spectral characteristics of PD is verified. This work provides important insights and possibilities for future fluorescent fiberoptic sensing and positioning for spatial PD detection and positioning using spectral characteristic peaks as detection quantities or excitations.
Conditions for Minimizing the Computational Complexity of the RCWA Calculation of the Diffraction Efficiency of Sawtooth Two-Layer Double-Relief Microstructures
Authors: Greisukh Grigoriy I. , Antonov Artem I. , Ezhov Evgeniy G. , Danilov Viktor A. , Usievich Boris A.
Published: July 2023

In this study, novel recommendations are presented and substantiated for selecting the number of modes and optical thicknesses of flat lattice slabs that make up microreliefs, which minimize the computational complexity of the rigorous coupled-wave analysis calculation of the diffraction efficiency (DE) of a sawtooth two-layer two-relief microstructure, while maintaining the specified reliability of the calculation results. The computational complexity can be controlled by allowing one or another level of oscillation of the DE curves, depending on the angle of incidence of the radiation incident on the microstructure. In particular, the complexity of the thousands of DE calculations in the optimization process can be reduced by using the proposed methodology as well as increased computational complexity to verify the accuracy of the solution obtained as a result of the implemented optimization.
Taxonomic Structure and Diversity of Benthic Diatoms (Bacillariophyta) in the Northern Shelf of the Black Sea
Authors: Nevrova Elena
Published: July 2023

The key role of benthic diatoms in coastal ecosystems highlights the relevance of studying their biodiversity and developing integrated biodiversity conservation measures. Benthic diatom richness in the northern part of the Black Sea was estimated based on both published data and our own data. The checklist of benthic diatoms includes 1100 species and intraspecific taxa, 953 species, 149 genera, 61 families, 32 orders, and 3 classes of Bacillariophyta. More than 280 species and intraspecific taxa, as well as 8 genera new to the diatom flora of the Black Sea and 7 species new to science, were revealed. An interregional analysis of the diversity of benthic Bacillariophyta along the coasts of Bulgaria, Romania, and Crimea, as well as the northwestern and northeastern parts of the Black Sea shelf, was carried out using the Taxonomic Distinctness Indices (TaxDI). The lowest TaxDI value for Crimea was closest to the average value expected for the Black Sea diatom flora, indicating a large proportion of polyspecies branches in the taxocene structure, which resulted in the maximum species richness (species/genus = 7) and led to a decrease in the vertical evenness of hierarchical tree. On the contrary, the highest TaxDI value was detected off the Romanian coast, which was caused by the significant proportion of mono- and oligospecies branches and minimal species saturation in the taxocene structure (species/genus = 4). We also identified some species with a high taxonomic exclusivity rank. If these species disappear, the entire architectonics of the Black Sea hierarchical tree of Bacillariophyta may be significantly altered. Such region-specific species are given the highest protection priority since they have great importance for gene pool conservation.
Straw Mulch Induced Indoleamines Alleviate Reproductive Depression in Cold Sensitive Hazelnut Cultivars
Authors: Ayyanath Murali-Mohan , Shukla Mukund R. , Hezema Yasmine S. , Saxena Praveen K.
Published: July 2023

Climate change is forcing physiological changes, especially in temperate trees, in which the reproduction phase has been affected harshly, eventually resulting in poor performance. Erratic fluctuations during the flowering periods, predominantly in cold-sensitive, yet industry-desired (sourced), hazelnut cultivars have been causing at least a 10-fold decline in the nut yield. Indoleamines have been noted to provide protection during such abiotic stress conditions. In this study, we investigated the potential involvement of the indoleamine pathway in countering reproductive depression in cold-sensitive hazelnuts by blanketing the ground with wheat straw mulch. The female flower ratio; titers of tryptophan, serotonin, and melatonin; and indoleamine pathway gene regulation were the endpoints for assessing the effects of straw mulch. In the preceding year, we noted that the occurrence of phenological events through the modulation of indoleamines was necessitated via percolation of snowmelt into the rootzone. Otherwise, reproductive depression was noted, especially in harsh conditions, such as ‘no snow’ or when the rootzone was covered with a plastic sheet to disallow water percolation. When cold-sensitive hazelnut cultivars that were subjected to such deleterious treatments in the preceding years’ experiments were treated with straw mulch, the female flower ratio was unaffected and remained on par with that of the cold-hardy locally adapted cultivars. Tryptophan accumulation improved in the (cold-sensitive) sourced cultivars treated with straw mulch and was available as serotonin to counter the cold stress. Lower titers of melatonin explained the slight improvement in female ratio in the sourced cultivars blanketed with straw mulch. ASMT gene regulation via straw mulch treatment emphasized its role in abiotic stress mitigation. A negative trend was noted when improved flowering was compared to the decreased expression of the ASMT gene. Horticultural changes, such as mulch, should provide mitigating solutions to relieve reproductive depression in cold-sensitive hazelnuts, alongside implications in other horticultural crops. The indoleamine toolkit (cellular markers) developed in this study provides insights into the mechanisms of cold sensitivity (abiotic stress) and plausible solutions, such as exogenous application of indoleamines, to propagate climate resilient plant materials with an enhanced capacity to mitigate abiotic stress conditions.
Genotypic Variation of Purple Rice in Response to Shading in Yield, Anthocyanin Content, and Gene Expression
Authors: Danpreedanan Nantapat , Yamuangmorn Supapohn , Jamjod Sansanee , Prom-u-thai Chanakan , Pusadee Tonapha
Published: July 2023

Purple rice (Oryza sativa L.) contains anthocyanin, which acts as an antioxidant and functional food for humans. The levels of anthocyanin growth and production in rice are mainly controlled by the availability of light. However, shade can affect anthocyanin biosynthesis genes. Therefore, the objective of this study was to determine the yield and anthocyanin content among four purple rice varieties, which provide the difference in colors of purple and green leaves. This study also evaluated gene expression affected by shading treatment to understand the relation of grain anthocyanin and expression level. This research was conducted using a split plot design using four levels of shading (levels of shading from anthesis to maturity) with three replications, no shading, 30% shading, 50% shading, and 70% shading, as the main plots and purple rice varieties as subplots, KJ CMU-107, K2, K4, and KDK10, from anthesis to maturity. Shading significantly decreased yield and yield components, but increased grain anthocyanin content. Nonetheless, the response of yield and grain anthocyanin content to shading did not show a significant different between purple and green leaf varieties. In addition, the level of OsDFR gene expression was different depending on the shading level in four rice varieties. The OsDFR gene presented the highest expression at shading levels of 30% for K4 and 50% for KDK10, while the expression of the OsDFR gene was not detected in the purple rice varieties with green leaves (KJ CMU-107 and K2). The response of grain anthocyanin and gene expression of OsDFR to light treatment did not show significantly differences between the purple and green leaf varieties, suggesting that the appearance of anthocyanin in leaves might be not related to anthocyanin synthesis in the grain. Taken together, the results suggest that some purple rice varieties were more suitable for planting under low light intensity based on a lower level of grain yield loss, strong shade tolerance, and high anthocyanin content in leaf and grain pericarp. However, it is necessary to explore the effects of light intensity on genes and intermediates in the anthocyanin synthesis pathway for further study.
Effects of Drought Stress on the Morphological Structure and Flower Organ Physiological Characteristics of Camellia oleifera Flower Buds
Authors: Guo Pu-Rui , Wu Ling-Li , Wang Ying , Liu Dan , Li Jian-An
Published: July 2023

Investigations on the impact of drought stress on the reproductive growth of C. oleifera have been relatively limited compared to the extensive research conducted on its nutritional growth. To study the effects of drought stress on the growth and development of C. oleifera flower buds, we investigated the effects of drought stress on the bud anatomical structure, relative water content, relative electrical conductivity, antioxidant enzyme activity, osmoregulation substance content, and hormone contents of C. oleifera using 4-year-old potted plants (‘Huaxin’ cultivar) as experimental materials. We observed C. oleifera flower bud shrinkage, faded pollen colour, shortened style length, decreased relative water content, increased relative electrical conductivity, and decreased pollen germination rate under drought stress. As the stress treatment duration increased, the malondialdehyde (MDA), soluble sugar (SS), soluble protein (SP), and proline (Pro) contents, as well as peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) activities increased. Moreover, the levels of the plant hormones indole acetic acid (IAA) and cytokinin (CTK) increased, whereas those of salicylic acid (SA) and jasmonic acid (JA) decreased, and those of abscisic acid (ABA) and gibberellin a3 (GA3) first increased and then decreased. Compared to the control group, the drought treatment group exhibited stronger antioxidant capacity, water regulation ability, and drought stress protection. These results indicate that C. oleifera is adaptable to drought-prone environments. The results of this study provide a theoretical basis for the evaluation of drought resistance in C. oleifera, as well as the development of water management strategies for cultivation.
Monitoring Genetic Erosion of Aromatic and Medicinal Plant Species in Alentejo (South Portugal)
Authors: Póvoa Orlanda , Lopes Violeta , Barata Ana Maria , Farinha Noémia
Published: July 2023

The main goal of this work was to study the genetic erosion risk of plants with aromatic, medicinal and gastronomic applications in Portugal, particularly in the Alentejo region. The target species were coriander (Coriandrum sativum L.), hart’s pennyroyal (Mentha cervina L.) and pennyroyal (Mentha pulegium L.). The methodology involved direct observations and surveys (2002/2003 and 2011). The GE formula applied in Hammer’s studies was used to estimate genetic erosion. The main factors causing genetic erosion were the primary drivers of biodiversity loss: habitat loss, invasive species, and overexploitation influenced by human intervention such as the clearing of watercourses, vegetation control, grazing and desertification. The results indicate a reduction in individuals per species in Alentejo, with a net erosion loss of 11% for M. pulegium, 32% for M. cervina and 33% for C. sativum. The overall loss of accessions (genetic erosion risk) was higher in cultivated accessions (33%) than in wild accessions (11%), with an annual genetic erosion rate of 3.7% and 1.2%, respectively. The annual risk of genetic erosion for M. pulegium accessions collected in a natural habitat was 0.6%, which is much lower than the 3.7% for M. cervina. These results consolidate the importance of collecting and conserving genetic resources.
Eukaryotic Translation Elongation Factor OsEF1A Positively Regulates Drought Tolerance and Yield in Rice
Authors: Gu Qing , Kang Junfang , Gao Shuang , Zhao Yarui , Yi Huan , Zha Xiaojun
Published: July 2023

Drought is one of the most serious stresses affecting rice growth. Drought stress causes accelerated senescence, reduced fertility, and subsequent reductions in crop yield. Eukaryotic translation elongation factor EF1A is an important multifunctional protein that plays an essential role in the translation of eukaryotic proteins. In this study, we localized and cloned the OsEF1A gene in rice (Oryza sativa) in order to clarify its role in drought tolerance and yield. Subcellular localization revealed that it was mainly localized to the cell membrane, cytoskeleton and nucleus. Compared with the wild-type, OsEF1A overexpressing transgenic plants had significantly more tillers and grains per plant, resulting in a significantly higher yield. Increases in the relative water content and proline content were also observed in the transgenic seedlings under drought stress, with a decrease in the malondialdehyde content, all of which are representative of drought tolerance. Taken together, these findings suggest that OsEF1A plays a positive regulatory role in rice nutritional development under drought stress. These findings will help support future studies aimed at improving yield and stress tolerance in rice at the molecular level, paving the way for a new green revolution.
A Study of the Molecular Regulatory Network of VcTCP18 during Blueberry Bud Dormancy
Authors: Li Ruixue , Ma Rui , Zheng Yuling , Zhao Qi , Zong Yu , Zhu Youyin , Chen Wenrong , Li Yongqiang , Guo Weidong
Published: July 2023

BRANCHED1 (BRC1) is a crucial member of the TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) gene family and is well known for playing a central role in shoot branching by controlling buds’ paradormancy. However, the expression characteristics and molecular regulatory mechanism of BRC1 during blueberry bud dormancy are unclear. To shed light on these topics, shoots of three blueberry cultivars with different chilling requirements (CRs) were decapitated in summer to induce paradormancy release and subjected to different levels of chilling in winter to induce endodormancy release. The results showed that the high-CR cultivar ‘Chandler’ had the strongest apical dominance among the three cultivars; additionally, the expression of VcTCP18, which is homologous to BRC1, was the highest under both the decapitation treatment and low-temperature treatment. The ‘Emerald’ cultivar, with a low CR, demonstrated the opposite trend. These findings suggest that VcTCP18 plays a negative regulatory role in bud break and that there may be a correlation between the CR and tree shape. Through yeast 1-hybrid (Y1H) assays, we finally screened 21 upstream regulatory genes, including eight transcription factors: zinc-finger homeodomain protein 1/4/5/9, MYB4, AP2-like ethylene-responsive transcription factor AINTEGUMENTA (ANT), ASIL2-like, and bHLH035. It was found that these upstream regulatory genes positively or negatively regulated the expression of VcTCP18 based on the transcriptome expression profile. In summary, this study enriched our understanding of the regulatory network of BRCl during bud dormancy and provided new insights into the function of BRC1.
A Boltzmann Electron Drift Diffusion Model for Atmospheric Pressure Non-Thermal Plasma Simulations
Authors: Popoli Arturo , Ragazzi Fabio , Pierotti Giacomo , Neretti Gabriele , Cristofolini Andrea
Published: July 2023

We introduce a fluid computational model for the numerical simulation of atmospheric pressure dielectric barrier discharge plasmas. Ion and neutral species are treated with an explicit drift diffusion approach. The Boltzmann relation is used to compute the spatial distribution of electrons as a function of the electrostatic potential and the ionic charge density. This technique, widely used to speed up particle and fluid models for low-pressure conditions, poses several numerical challenges for high-pressure conditions and large electric field values typical of applications involving atmospheric-pressure plasmas. We develop a robust algorithm to solve the non-linear electrostatic Poisson problem arising from the Boltzmann electron approach under AC electric fields based on a charge-conserving iterative computation of the reference electric potential and electron density. We simulate a volumetric reactor in dry air, comparing the results yielded by the proposed method with those obtained when the drift diffusion approach is used for all charged species, including electrons. We show that the proposed methodology retains most of the physical information provided by the reference modeling approach while granting a substantial advantage in terms of computation time.
Process Design and Techno-ECONOMIC Evaluation of a Decarbonized Cement Production Process Using Carbon Capture and Utilization
Authors: Wu Wei , Jian Zhong-Lin , Chou Bang-Yan , You Chun-Yang , Kuo Yu-Ning
Published: July 2023

To address a decarbonized cement production process (DCPP), a calcium looping process is connected to an industrial cement production process (CPP) for capturing CO2 by 93.5~96%. Since the captured CO2 purity is up to 99.9 wt%, the carbon capture and utilization (CCU) process is connected to generate the additional products of urea and methanol. An integration of DCPP and CCU, named the DCPP-based polygeneration system, is being developed for three scenarios. To meet the power demand for producing high-purity hydrogen and oxygen, Scenario 1 adopts water electrolysis and the full green electricity grid; Scenario 2 adopts the Cu-Cl thermochemical cycle and the partial green electricity grid; and Scenario 3 adopts water electrolysis and the heat recovery steam generator (HRSG). Through the techno-economic analysis and comparisons, the CO2 avoided costs of three scenarios are estimated between 16.53 and 21.42 USD/ton, which are lower than the conventional DCPP of around 40 USD/ton. It is due to the fact that the polygeneration scheme could reduce the LCOP (levelized cost of producing 1 ton of clinker) due to the production of valorized products. It is noted that Scenario 2 is superior to other scenarios since the RenE2P cost in Scenario 2 is lower than it is in Scenario 1 and the captured CO2 rate in Scenario 2 is lower than it is in Scenario 3.
Batch and Continuous Column Adsorption of p-Nitrophenol onto Activated Carbons with Different Particle Sizes
Authors: Ledesma Beatriz , Sabio Eduardo , González-García Carmen María , Román Silvia , Fernandez Maria Emilia , Bonelli Pablo , Cukierman Ana L.
Published: July 2023

The study focused on investigating the solvent adsorption of p-Nitrophenol (PNP) onto activated carbons for wastewater treatment. It explored the influence of adsorbate concentration and adsorbent size on equilibrium isotherms and removal rates to develop efficient adsorption processes. The study examined adsorption isotherms under equilibrium conditions utilizing both the Langmuir and Double-Langmuir models and the Dubinin–Radushkevich equation. Remarkably, all the models demonstrated equally excellent fitting to the experimental data. Kinetics of PNP adsorption were investigated using pseudo-first-order, pseudo-second-order, and intraparticle diffusion kinetic models. This provided insights into the dominant adsorption mechanism and mass transfer phenomena, aiding the design of efficient wastewater treatment processes. Strong correlations (correlation coefficients > 0.9) were found between the models and experimental data for three types of activated carbons under batch conditions. This validation enhances the reliability and applicability of the models, supporting their practical use. The study also observed a slight increase in maximum adsorption capacity (qmax) with decreasing particle size, although there is not a significant difference: 340, 350, and 365 mg·g−1, for CB-L, CB-M, and CB-S, respectively. This insight helps in selecting appropriate activated carbon for effective PNP removal, considering both adsorption capacity and particle size. Furthermore, the analysis of PNP adsorption under dynamic conditions in fixed-bed columns highlighted the significance of inlet velocity and carbon mass in determining breakthrough time, with particle size playing a secondary role. This information aids in optimizing the design and operation of fixed-bed adsorption systems for efficient PNP removal. In summary, this study’s significant contributions lie in enhancing our understanding of PNP adsorption in wastewater treatment. By investigating equilibrium isotherms, kinetics, and mass transfer phenomena, it provides validated models, insights into adsorption capacity and particle size, and practical guidance for dynamic adsorption systems. These findings contribute to the development of efficient and sustainable wastewater treatment methods.