bluuebunny/crossref_metadata_2025 huggingface dataset:
Peak Power Control in Multicarrier Communications
Authors: Litsyn Simon
Published: December 2009

Peak signal power is an important factor in the implementation of multicarrier (MC) modulation schemes, like OFDM, in wireless and wireline communication systems. This 2007 book describes tools necessary for analyzing and controlling the peak-to-average power ratio in MC systems, and how these techniques are applied in practical designs. The author starts with an overview of multicarrier signals and basic tools and algorithms, before discussing properties of MC signals in detail: discrete and continuous maxima; statistical distribution of peak power; codes with constant peak-to-average power ratio are all covered, concluding with methods to decrease peak power in MC systems. Current knowledge, problems, methods and definitions are summarized using rigorous mathematics, with an overview of the tools for the engineer. The book is aimed at graduate students and researchers in electrical engineering, computer science and applied mathematics, and practitioners in the telecommunications industry.
This Land, This Nation
Authors: Phillips Sarah T.
Published: December 2009

This 2007 book combines political with environmental history to present conservation policy as a critical arm of New Deal reform, one that embodied the promises and limits of midcentury American liberalism. It interprets the natural resource programs of the 1930s and 1940s as a set of federal strategies aimed at rehabilitating the economies of agricultural areas. The New Dealers believed that the country as a whole would remain mired in depression as long as its farmers remained poorer than its urban residents, and these politicians and policymakers set out to rebuild rural life and raise rural incomes with measures tied directly to conservation objectives - land retirement, soil restoration, flood control, and affordable electricity for homes and industries. In building new constituencies for the environmental initiatives, resource administrators and their liberal allies established the political justification for an enlarged federal government and created the institutions that shaped the contemporary rural landscape.
The Architecture of Government
Authors: Treisman Daniel
Published: December 2009

Since the days of Montesquieu and Jefferson, political decentralization has been seen as a force for better government and economic performance. It is thought to bring government 'closer to the people', nurture civic virtue, protect liberty, exploit local information, stimulate policy innovation, and alleviate ethnic tensions. Inspired by such arguments, and generously funded by the major development agencies, countries across the globe have been racing to devolve power to local governments. This book re-examines the arguments that underlie the modern faith in decentralization. Using logical analysis and formal modeling, and appealing to numerous examples, it shows that most are based on vague intuitions or partial views that do not withstand scrutiny. A review of empirical studies of decentralization finds these as inconclusive and mutually contradictory as the theories they set out to test.
Supersymmetry in Particle Physics
Authors: Aitchison Ian
Published: December 2009

Supersymmetry represents the culmination of the search for fundamental symmetries that has dominated particle physics for 50 years. Traditionally, the constituents of matter (fermions) were regarded as different from the particles (bosons) transmitting the forces between them. In supersymmetry, fermions and bosons are unified. Intended for graduate students in particle physics, and researchers in experimental and phenomenological supersymmetry, this textbook, first published in 2007, provides a simple introduction to a previously formidably technical field. Its elementary, practical treatment brings readers to the frontier of contemporary research, in particular the experiments at the Large Hadron Collider. Theories are constructed through an intuitive 'trial and error' approach. Basic elements of spinor formalism and superfields are introduced, allowing readers to access more advanced treatments. Emphasis is placed on physical understanding, and on detailed derivations of important steps. Many short exercises are included, making for a valuable and accessible self-study tool.
Media Argumentation
Authors: Walton Douglas
Published: December 2009

Media argumentation is a powerful force in our lives. From political speeches to television commercials to war propaganda, it can effectively mobilize political action, influence the public, and market products. This book presents a new and systematic way of thinking about the influence of mass media in our lives, showing the intersection of media sources with argumentation theory, informal logic, computational theory, and theories of persuasion. Using a variety of case studies that represent arguments that typically occur in the mass media, Douglas Walton demonstrates how tools recently developed in argumentation theory can be usefully applied to the identification, analysis, and evaluation of media arguments. He draws upon the most recent developments in artificial intelligence, including dialogical theories of argument, which he developed, as well as speech act theory. Each chapter presents solutions to problems central to understanding, analyzing, and criticizing media argumentation.
Problems of the Self
Authors: Williams Bernard
Published: December 2009

This is a volume of philosophical studies, centred on problems of personal identity and extending to related topics in the philosophy of mind and moral philosophy.
The Theology of the Acts of the Apostles
Authors: Jervell Jacob
Published: December 2009

Who are the people of God? Luke's purposes in the Acts of the Apostles are to identify the church, to establish the legitimacy of its gospel and to demonstrate that God was an active force in history. He wanted to show that the communities of Jewish and Gentile Christians are the true heirs of God's promises to Israel. He gives the history of the early church from the last decades of the first century as the communities become separated from their Jewish origins, and Paul plays the lead role. Acts offers an apologetic for the mixed mission of the church: to Jews and Gentiles. Luke was an eyewitness to some of what he reports, but his authorship and views have been questioned. This is a theological interpretation of the history of the church within history: Luke is an artist, a narrator rather than a systematic theologian, but writes about the roles of God, Christ and the Holy Spirit, and of the church.
Sport, Economy and Society in Britain 1750–1914
Authors: Tranter Neil
Published: December 2009

This book provides a concise, up-to-date survey of one of the most dramatic changes in the cultural life of Victorian and Edwardian Britain, the radical transformation which occurred in the extent and nature of its participation in sport. Neil Tranter focuses on the issues which have attracted most interest from historians of sport and poses a number of important questions: did levels of involvement in sport increase or decrease during the initial stages of urban-industrialisation? When did the new sporting culture first emerge, and what were its principal features and the mechanisms through which it spread? What were the main aims of the participants and supporters, and to what extent were these aims achieved? The author also discusses the economic consequences of this cultural change and the examines the role of women in this sporting 'revolution' and asks why their participation was so much more restricted than that of men.
New Essays on The Education of Henry Adams
Authors: Rowe John Carlos
Published: December 2009

This volume in The American Novel series addresses the established reputation of The Education of Henry Adams as a classic work of American autobiography and canonical work of American literature. Examining The Education in terms of early twentieth-century American attitudes to education, gender, US foreign policy, and historiography, these essays add considerably to our understanding of the Education as an expression of its time. The approaches of the four contributors - John Carlos Rowe, Brook Thomas, Martha Banta, and Howard Horwitz - complement each other, even though the specific topic explored by each scholar is distinctly different from the others. The result is a remarkably coherent volume that explains in original ways the continuing importance of The Education of Henry Adams as literature and history.
Propositional Attitudes
Authors: Richard Mark
Published: December 2009

This book makes a stimulating contribution to the philosophy of language and philosophy of mind. It begins with a spirited defence of the view that propositions are structured and that propositional structure is 'psychologically real'. The author then develops a subtle view of propositions and attitude ascription. The view is worked out in detail with attention to such topics as the semantics of conversations, iterated attitude ascriptions, and the role of propositions as bearers of truth. Along the way important issues in the philosophy of mind are addressed. Though intended primarily for professional philosophers and graduate students the book will also interest cognitive scientists and linguists.
Market Microstructure
Authors: Spulber Daniel F.
Published: December 2009

This book presents a theory of the firm based on its economic role as an intermediary between customers and suppliers. Professor Spulber demonstrates how the intermediation theory of the firm explains firm formation by showing how they arise in a market equilibrium. In addition, the theory helps explain how markets work by showing how firms select market-clearing prices. Models of intermediation and market microstructure from microeconomics and finance shed considerable light on the formation and market-making activities of firms. The intermediation theory of the firm is compared to existing economic theories of the firm including the neoclassical, industrial organization, transaction cost, and principal-agent models.
Democracy and Moral Conflict
Authors: Talisse Robert B.
Published: December 2009

Why democracy? Most often this question is met with an appeal to some decidedly moral value, such as equality, liberty, dignity or even peace. But in contemporary democratic societies, there is deep disagreement and conflict about the precise nature and relative worth of these values. And when democracy votes, some of those who lose will see the prevailing outcome as not merely disappointing, but morally intolerable. How should citizens react when confronted with a democratic result that they regard as intolerable? Should they revolt, or instead pursue democratic means of social change? In this book, Robert Talisse argues that each of us has reasons to uphold democracy - even when it makes serious moral errors - and that these reasons are rooted in our most fundamental epistemic commitments. His original and compelling study will be of interest to a wide range of readers in political philosophy and political theory.
Inside-Out Houses: Urban Belonging and Imagined Futures in Dakar, Senegal
Authors: Melly Caroline
Published: December 2009

In every neighborhood of Dakar, Senegal, large houses in various stages of construction stand as witnesses to and evidence of transnational movements of labor and capital. These ambitious building projects, funded by Senegalese migrants living and working abroad, have utterly transformed the city landscape, and their pervasiveness leads many Dakarois to assume that everyone must be migrating. Intended as eventual family homes, investment properties, or a combination of the two, the innovative layouts and architectural flourishes of these not-yet houses echo lives lived elsewhere while drawing on local aesthetics and approaches to spatial design. Though some houses seem to near completion within just a year or two, most structures linger for several years or even a decade, slowly eroding as families and hired contractors wait for money transfers from abroad. Some constructions boast newly laid bricks or fresh paint, while others are obscured by overgrown vegetation and debris.
I Want More of You: The Politics of Christian Eroticism in Postwar Guatemala
Authors: O'Neill Kevin Lewis
Published: December 2009

The image appeared on the cover of a Sunday bulletin, produced and distributed by one of Guatemala City's most conservative neo-Pentecostal mega-churches. The picture presented the face of a young teenage girl, her eyes closed, lips wet, and skin kissed by a soft, transcendent light; the young woman's head was even tilted to the side in what Jacques Lacan would calljouissance(1998). Across her pink lips read Psalm 4:6: “In peace, I lay myself down.” This image, stitched together by the church's media relations department, makes a sly reference to Gian Lorenzo Bernini's sculpture,St. Teresa in Ecstasy(1652). The statue in Rome presents one of Teresa of Ávila's (1515–1582) mystical experiences of God, which the sixteenth-century Spanish saint narrates with unblinkingly erotic imagery. In her autobiography, St. Teresa writes how “the great love of God” often left her “utterly consumed,” “penetrated to [her] entrails,” and made her “utter several moans” for both the “intense pain” and its “sweetness” (Peers 1927: 197). With St. Teresa in mind, my own reaction to the church bulletin parroted Jacques Lacan's response to Bernini's statue. “She's coming,” Lacan commented, “There's no doubt about it” (1998: 76).
Frontier Androgyny: An Archetypal Female Hero in The Adventures of Daniel Boone
Authors: WILSON SMITH SANDRA
Published: December 2009

Influential American studies scholars of the mid-twentieth century, such as R. W. B. Lewis, Henry Nash Smith, and Leslie A. Fiedler, focussed attention on a mythic American character, the frontiersman who penetrates the wilderness. These critics provided analyses of figures such as Daniel Boone and his fictional counterpart, Natty Bumppo, and discussed the power the romanticized western frontier had over the American imagination. Their observations were accurate, as far as they went. However, these critics did not acknowledge the many narratives in which a female character conquers the frontier. The assumption seemed to be that the literary female figure belonged in the parlor with her sewing basket and not in the forest with her weapon. Unfortunately, this incomplete assessment of the frontier adventure genre is still in evidence today. In this essay, I work towards the development of a more complete understanding of the American frontier story and point out that, even in the iconic John Filson/John Trumbull Boone tale, we find a mini-narrative involving a female hero who triumphs over the “savage” forces of the wilderness. This female figure became a cultural archetype, and similar versions of her story were repeated in countless captivity and western adventure anthologies, almanacs, and the like for the next seventy years. The popularity of this frontier narrative featuring a strong, violent female figure suggests that readers were accepting of the idea of an active, aggressive woman, at least while she was contending with chaotic forces in the wilderness. The popularity of this kind of narrative also undercuts the traditional gender paradigm (the nurturing passive female versus the active aggressive male) too often imposed by scholars on antebellum American letters and culture.
Colloidal Palladium Particles of Different Shapes for Electron Microscopy Labeling
Authors: Meyer Daryl A. , Oliver Julie A. , Albrecht Ralph M.
Published: December 2009

The immunogold technique is a valuable method for labeling cellular macromolecules. However, multiple labeling using colloidal gold (cAu) nanoparticles of different sizes presents certain drawbacks; namely, as particle size increases, there is a decreased labeling efficiency and diminished spatial resolution with respect to the locations of labeled epitopes. Both concerns also limit the utility of heavy metal particles for comparative analysis of labeling densities. To minimize the variables due to differential labeling efficiencies, the best solution would be to conduct multiple labeling with particles of similar size. Consequently, some parameter other than size is necessary to distinguish each label type. In this study, we report the synthesis of colloidal palladium (cPd) nanoparticles of similar size but having two distinct shapes, umbonate and faceted, which are readily distinguishable from spherical colloidal gold particles. Their utility and fidelity as labels using a human platelet whole-mount model is also demonstrated.
Crenarchaeol dominates the membrane lipids of Candidatus Nitrososphaera gargensis, a thermophilic Group I.1b Archaeon
Authors: Pitcher Angela , Rychlik Nicolas , Hopmans Ellen C , Spieck Eva , Rijpstra W Irene C , Ossebaar Jort , Schouten Stefan , Wagner Michael , Sinninghe Damsté Jaap S
Published: December 2009

Analyses of archaeal membrane lipids are increasingly being included in ecological studies as a comparatively unbiased complement to gene-based microbiological approaches. For example, crenarchaeol, a glycerol dialkyl glycerol tetraether (GDGT) with a unique cyclohexane moiety, has been postulated as biomarker for ammonia-oxidizing Archaea (AOA). Crenarchaeol has been detected in Nitrosopumilus maritimus and ‘Candidatus Nitrosocaldus yellowstonii’ representing two of the three lineages within the Crenarchaeota containing described AOA. In this paper we present the membrane GDGT composition of ‘Candidatus Nitrososphaera gargensis’, a moderately thermophilic AOA, and the only cultivated Group I.1b Crenarchaeon. At a cultivation temperature of 46 °C, GDGTs of this organism consisted primarily of crenarchaeol, its regioisomer, and a novel GDGT. Intriguingly, ‘Ca. N. gargensis’ is the first cultivated archaeon to synthesize substantial amounts of the crenarchaeol regioisomer, a compound found in large relative abundances in tropical ocean water and some soils, and an important component of the TEX86 paleothermometer. Intact polar lipid (IPL) analysis revealed that ‘Ca. N. gargensis’ synthesizes IPLs similar to those reported for the Goup I.1a AOA, Nitrosopumilus maritimus SCMI, in addition to IPLs containing uncharacterized headgroups. Overall, the unique GDGT composition of ‘Ca. N. gargensis’ extends the known taxonomic distribution of crenarchaeol synthesis to the Group I.1b Crenarchaeota, implicating this clade as a potentially important source of crenarchaeol in soils and moderately high temperature environments. Moreover, this work supports the hypothesis that crenarchaeol is specific to all AOA and highlights specific lipids, which may prove useful as biomarkers for ‘Ca. N. gargensis’-like AOA.
Sociodemographic, Perinatal, Behavioral, and Psychosocial Predictors of Weight Retention at 3 and 12 Months Postpartum
Authors: Siega‐Riz Anna Maria , Herring Amy H. , Carrier Kathryn , Evenson Kelly R. , Dole Nancy , Deierlein Andrea
Published: December 2009

Postpartum weight retention plays an important role in the pathway leading to obesity among women of childbearing age. The objective of this study was to examine predictors of moderate (1–10 pounds) and high (>10 pounds) postpartum weight retention using data from a prospective pregnancy cohort that followed women into the postpartum period; n = 688 and 550 women at 3 and 12 months, respectively. Analysis included descriptive statistics and predictive modeling using log‐binomial techniques. The average weight retained at 3 and 12 months postpartum in this population was 9.4 lb (s.d. = 11.4) and 5.7 lb (s.d. = 13.2), respectively. At 3 months postpartum, prepregnancy weight, gestational weight gain, and hours slept during the night were associated with moderate or high weight retention, whereas having an infant hospitalized after going home and scoring in the upper 75th percentile of the Eating Attitudes Test (EAT) were associated only with high weight retention. At 12 months postpartum, prepregnancy weight, gestational weight gain, and maternal education were associated with moderate weight retention; and gestational weight gain, maternal age, race, employment status, and having an infant hospitalized at birth were associated with high weight retention. The results of this study illustrate the importance of prepregnancy weight and gestational weight gain in predicting postpartum weight retention. Furthermore, given the lack of successful intervention studies that exist to date to help women lose weight in the postpartum period, the results of this study may help to inform future interventions that focus on such aspects as hours of sleep, dealing with stress associated with a hospitalized infant, and nonclinical eating disorder symptomatology.
Label-free detection of protein-protein interactions using a calmodulin-modified nanowire transistor
Authors: Lin Tsung-Wu , Hsieh Po-Jen , Lin Chih-Lung , Fang Yi-Ya , Yang Jia-Xun , Tsai Chia-Chang , Chiang Pei-Ling , Pan Chien-Yuan , Chen Yit-Tsong
Published: December 2009

In this study, we describe a highly sensitive and reusable silicon nanowire field-effect transistor for the detection of protein-protein interactions. This reusable device was made possible by the reversible association of glutathione S-transferase-tagged calmodulin with a glutathione modified transistor. The calmodulin-modified transistor exhibited selective electrical responses to Ca2+(≥1 μM) and purified cardiac troponin I (∼7 nM); the change in conductivity displayed a linear dependence on the concentration of troponin I in a range from 10 nM to 1 μM. These results are consistent with the previously reported concentration range in which the dissociation constant for the troponin I-calmodulin complex was determined. The minimum concentration of Ca2+required to activate calmodulin was determined to be 1 μM. We have also successfully demonstrated that the N-type Ca2+channels, expressed by cultured 293T cells, can be recognized specifically by the calmodulin-modified nanowire transistor. This sensitive nanowire transistor can serve as a high-throughput biosensor and can also substitute for immunoprecipitation methods used in the identification of interacting proteins.
Limited contribution of stem-loop potential to symmetry of single-stranded genomic DNA
Authors: Zhang Shang-Hong , Huang Ya-Zhi
Published: December 2009

Motivation: The phenomenon of strand symmetry, which may provide clues to genome evolution, exists in all prokaryotic and eukaryotic genomes studied. Several possible mechanisms for its origins have been proposed, including: no strand biases for mutation and selection, strand inversion and selection of stem-loop structures. However, the relative contributions of these mechanisms to strand symmetry are not clear. In this article, we studied specifically the role of stem-loop potential of single-stranded DNA in strand symmetry.

Results: We analyzed the complete genomes of 90 prokaryotes. We found that most oligonucleotides (pentanucleotides and higher) do not have a reverse complement in close proximity in the genomic sequences. Combined with further analysis, we conclude that the contribution of the widespread stem-loop potential of single-stranded genomic DNA to the formation and maintenance of strand symmetry would be very limited, at least for higher-order oligonucleotides. Therefore, other possible causes for strand symmetry must be taken into account to a deeper degree.

Contacts:  lsszsh@mail.sysu.edu.cn; molevol@mail.sysu.edu.cn

Supplementary information:  Supplementary data are available at Bioinformatics online.
Young children with autism spectrum disorder use predictive eye movements in action observation
Authors: Falck-Ytter Terje
Published: December 2009

Does a dysfunction in the mirror neuron system (MNS) underlie the social symptoms defining autism spectrum disorder (ASD)? Research suggests that the MNS matches observed actions to motor plans for similar actions, and that these motor plans include directions for predictive eye movements when observing goal-directed actions. Thus, one important question is whether children with ASD use predictive eye movements in action observation. Young children with ASD as well as typically developing children and adults were shown videos in which an actor performed object-directed actions (human agent condition). Children with ASD were also shown control videos showing objects moving by themselves (self-propelled condition). Gaze was measured using a corneal reflection technique. Children with ASD and typically developing individuals used strikingly similar goal-directed eye movements when observing others’ actions in the human agent condition. Gaze was reactive in the self-propelled condition, suggesting that prediction is linked to seeing a hand–object interaction. This study does not support the view that ASD is characterized by a global dysfunction in the MNS.
The effectiveness of an air cleaner in controlling droplet/aerosol particle dispersion emitted from a patient's mouth in the indoor environment of dental clinics
Authors: Chen Chun , Zhao Bin , Cui Weilin , Dong Lei , An Na , Ouyang Xiangying
Published: December 2009

Dental healthcare workers (DHCWs) are at high risk of occupational exposure to droplets and aerosol particles emitted from patients' mouths during treatment. We evaluated the effectiveness of an air cleaner in reducing droplet and aerosol contamination by positioning the device in four different locations in an actual dental clinic. We applied computational fluid dynamics (CFD) methods to solve the governing equations of airflow, energy and dispersion of different-sized airborne droplets/aerosol particles. In a dental clinic, we measured the supply air velocity and temperature of the ventilation system, the airflow rate and the particle removal efficiency of the air cleaner to determine the boundary conditions for the CFD simulations. Our results indicate that use of an air cleaner in a dental clinic may be an effective method for reducing DHCWs' exposure to airborne droplets and aerosol particles. Further, we found that the probability of droplet/aerosol particle removal and the direction of airflow from the cleaner are both important control measures for droplet and aerosol contamination in a dental clinic. Thus, the distance between the air cleaner and droplet/aerosol particle source as well as the relative location of the air cleaner to both the source and the DHCW are important considerations for reducing DHCWs' exposure to droplets/aerosol particles emitted from the patient's mouth during treatments.
Decline in top predator body size and changing climate alter trophic structure in an oceanic ecosystem
Authors: Shackell Nancy L. , Frank Kenneth T. , Fisher Jonathan A. D. , Petrie Brian , Leggett William C.
Published: December 2009

Globally, overfishing large-bodied groundfish populations has resulted in substantial increases in their prey populations. Where it has been examined, the effects of overfishing have cascaded down the food chain. In an intensively fished area on the western Scotian Shelf, Northwest Atlantic, the biomass of prey species increased exponentially (doubling time of 11 years) even though the aggregate biomass of their predators remained stable over 38 years. Concomitant reductions in herbivorous zooplankton and increases in phytoplankton were also evident. This anomalous trophic pattern led us to examine how declines in predator body size (approx. 60% in body mass since the early 1970s) and climatic regime influenced lower trophic levels. The increase in prey biomass was associated primarily with declines in predator body size and secondarily to an increase in stratification. Sea surface temperature and predator biomass had no influence. A regression model explained 65 per cent of prey biomass variability. Trait-mediated effects, namely a reduction in predator size, resulted in a weakening of top predation pressure. Increased stratification may have enhanced growing conditions for prey fish. Size-selective harvesting under changing climatic conditions initiated a trophic restructuring of the food chain, the effects of which may have influenced three trophic levels.
Human genetic variation recognizes functional elements in noncoding sequence
Authors: Lomelin David , Jorgenson Eric , Risch Neil
Published: December 2009

Noncoding DNA, particularly intronic DNA, harbors important functional elements that affect gene expression and RNA splicing. Yet, it is unclear which specific noncoding sites are essential for gene function and regulation. To identify functional elements in noncoding DNA, we characterized genetic variation within introns using ethnically diverse human polymorphism data from three public databases—PMT, NIEHS, and SeattleSNPs. We demonstrate that positions within introns corresponding to known functional elements involved in pre-mRNA splicing, including the branch site, splice sites, and polypyrimidine tract show reduced levels of genetic variation. Additionally, we observed regions of reduced genetic variation that are candidates for distance-dependent localization sites of functional elements, possibly intronic splicing enhancers (ISEs). Using several bioinformatics approaches, we provide additional evidence that supports our hypotheses that these regions correspond to ISEs. We conclude that studies of genetic variation can successfully discriminate and identify functional elements in noncoding regions. As more noncoding sequence data become available, the methods employed here can be utilized to identify additional functional elements in the human genome and provide possible explanations for phenotypic associations.
Kinetic and Spectral Resolution of Multiple Nonphotochemical Quenching Components in Arabidopsis Leaves
Authors: Lambrev Petar H. , Nilkens Manuela , Miloslavina Yuliya , Jahns Peter , Holzwarth Alfred R.
Published: December 2009

Using novel specially designed instrumentation, fluorescence emission spectra were recorded from Arabidopsis (Arabidopsis thaliana) leaves during the induction period of dark to high-light adaptation in order to follow the spectral changes associated with the formation of nonphotochemical quenching. In addition to an overall decrease of photosystem II fluorescence (quenching) across the entire spectrum, high light induced two specific relative changes in the spectra: (1) a decrease of the main emission band at 682 nm relative to the far-red (750–760 nm) part of the spectrum (Δ F682); and (2) an increase at 720 to 730 nm (Δ F720) relative to 750 to 760 nm. The kinetics of the two relative spectral changes and their dependence on various mutants revealed that they do not originate from the same process but rather from at least two independent processes. The Δ F720 change is specifically associated with the rapidly reversible energy-dependent quenching. Comparison of the wild-type Arabidopsis with mutants unable to produce or overexpressing the PsbS subunit of photosystem II showed that PsbS was a necessary component for Δ F720. The spectral change Δ F682 is induced both by energy-dependent quenching and by PsbS-independent mechanism(s). A third novel quenching process, independent from both PsbS and zeaxanthin, is activated by a high turnover rate of photosystem II. Its induction and relaxation occur on a time scale of a few minutes. Analysis of the spectral inhomogeneity of nonphotochemical quenching allows extraction of mechanistically valuable information from the fluorescence induction kinetics when registered in a spectrally resolved fashion.
Antibodies to citrullinated proteins: molecular interactions and arthritogenicity
Authors: Uysal Hüseyin , Nandakumar Kutty Selva , Kessel Christoph , Haag Sabrina , Carlsen Stefan , Burkhardt Harald , Holmdahl Rikard
Published: December 2009

Summary:  The discovery of antibodies specific for citrullinated protein epitopes [anti‐citrullinated protein antibodies (ACPAs)] is a hallmark for the diagnosis and prognosis of rheumatoid arthritis (RA) and will also be a useful tool for understanding the fundamental pathologic processes. There are several essential questions pertaining to ACPA that remain to be explored, such as understanding the early specificity of the underlying T‐cell recognition, whether the production of ACPA is a primary or secondary process, and in the event of such antibodies being arthritogenic, whether they could possibly regulate the disease development. To answer these questions, animal models are needed, but unfortunately ACPA is not a prominent feature of any of the classical animal models of RA. However, we showed recently that ACPA can be isolated from animals susceptible to collagen‐induced arthritis that are specific for citrullinated type II collagen (CII). The citrulline specificity could be visualized, and the specificity is determined primarily by a direct interaction with citrulline. We also demonstrated that these antibodies are specific for the citrullinated epitopes and are pathogenic in vivo. A new hypothesis to explain how inflammation in RA can be directed to cartilaginous joints and be self‐perpetuating is suggested, which involves recognition of post‐translational modifications (glycosylation and citrullination) on CII by T and B cells that can have both arthritogenic and regulatory consequences.
Suppressive functions of activated B cells in autoimmune diseases reveal the dual roles of Toll‐like receptors in immunity
Authors: Lampropoulou Vicky , Calderon‐Gomez Elisabeth , Roch Toralf , Neves Patricia , Shen Ping , Stervbo Ulrik , Boudinot Pierre , Anderton Stephen M. , Fillatreau Simon
Published: December 2009

Summary:  B lymphocytes contribute to immunity through production of antibodies, antigen presentation to T cells, and secretion of cytokines. B cells are generally considered in autoimmune diseases as drivers of pathogenesis. This view is certainly justified, given the successful utilization of the B cell‐depleting reagent rituximab in patients with rheumatoid arthritis or other autoimmune pathologies. In a number of cases, however, the depletion of B cells led to an exacerbation of symptoms in patients with autoimmune disorders. In a similar manner, mice lacking B cells can develop an aggravated course of disease in several autoimmune models. These paradoxical observations are now explained by the concept that activated B cells can suppress immune responses through the production of cytokines, especially interleukin‐10. Here, we review the stimulatory signals that induce interleukin‐10 secretion and suppressive functions in B cells and the phenotype of the B cells with such characteristics. Finally, we formulate a model explaining how this process of immune regulation by activated B cells can confer advantageous properties to the immune system in its combat with pathogens. Altogether, this review proposes that B‐cell‐mediated regulation is a fundamental property of the immune system, with features of great interest for the development of new cell‐based therapies for autoimmune diseases.
Securing a Sustainable Future for Children and Young People
Authors: Renton Zoë , Butcher Joanne
Published: December 2009

This article outlines why sustainable development matters for children and young people, and explores the relevant policy context in England and the UK. It asks whether enough is being carried out by central government to secure a more sustainable future for, and with, today’s children. More is needed at the national policy level to: embed sustainable living principles within a range of policy frameworks affecting children and young people; provide a clear strategy for empowering children and young people to lead change and take action on climate change and environmental issues; and address the impact of environmental degradation on disadvantaged groups of children and young people.
Major vault protein forms complexes with hypoxia‐inducible factor (HIF)‐1α and reduces HIF‐1α level in ACHN human renal adenocarcinoma cells
Authors: Iwashita Ken‐ichi , Ikeda Ryuji , Takeda Yasuo , Sumizawa Tomoyuki , Furukawa Tatsuhiko , Yamaguchi Tatsuya , Akiyama Shin‐ichi , Yamada Katsushi
Published: December 2009

Vaults are evolutionarily highly conserved ribonucleoprotein (RNP) particles with a hollow barrel‐like structure. Although roles in multidrug resistance and innate immunity have been suggested, the physiological function of vaults remains unclear. Major vault protein (MVP), the main component of the vault particle, has been reported to be induced by hypoxia. However, there are no reports about the effect of vaults on cellular responses to hypoxia. We thus examined whether vaults are implicated in cellular responses to hypoxia. In this study, we focused on hypoxia‐inducible factor‐1α (HIF‐1α), which is a master regulator of hypoxic responses, and found that: (i) MVP knockdown by RNA interference increases HIF‐1α protein levels induced by hypoxia and hypoxia mimetics; (ii) MVP knockdown does not affect HIF‐1α mRNA levels, but decreases the ubiquitination and degradation of HIF‐1α protein; and (iii) vaults form complexes with HIF‐1α, PHD2, and pVHL. Taken together, these results suggest that vaults function as scaffolds in HIF‐1α degradation pathway and promote the ubiquitination and degradation of HIF‐1α.

(Cancer Sci 2010; 101: 920–926)
Influence of plant species and soil conditions on plant–soil feedback in mixed grassland communities
Authors: Harrison Kathryn A. , Bardgett Richard D.
Published: December 2009

1. Our aim was to explore plant–soil feedback in mixed grassland communities and its significance for plant productivity and community composition relative to abiotic factors of soil type and fertility.

2. We carried out a 4‐year, field‐based mesocosm experiment to determine the relative effects of soil type, historic management intensity and soil conditioning by a wide range of plant species of mesotrophic grassland on the productivity and evenness of subsequent mixed communities.

3. The study consisted of an initial soil conditioning phase, whereby soil from two locations each with two levels of management intensity was conditioned with monocultures of nine grassland species, and a subsequent feedback phase, where mixed communities of the nine species were grown in conditioned soil to determine relative effects of experimental factors on the productivity and evenness of mixed communities and individual plant species performance.

4. In the conditioning phase of the experiment, individual plant species differentially influenced soil microbial communities and nutrient availability. However, these biotic effects were much less important as drivers of soil microbial properties and nutrient availability than were abiotic factors of soil type and fertility.

5. Significant feedback effects of conditioning were detected during the second phase of the study in terms of individual plant growth in mixed communities. These feedback effects were generally independent of soil type or fertility, and were consistently negative in nature. In most cases, individual plant species performed less well in mixed communities planted in soil that had previously supported their own species.

6. Synthesis. These findings suggest that despite soil abiotic factors acting as major drivers of soil microbial communities and nutrient availability, biotic interactions in the form of negative feedback play a significant role in regulating individual plant performance in mixed grassland communities across a range of soil conditions.
Chemical Activation with a Combination of Ionomycin and Dehydroleucodine for Production of Parthenogenetic, ICSI and Cloned Bovine Embryos
Authors: Vichera G , Alfonso J , Duque CC , Silvestre MA , Pereyra‐Bonnet F , Fernández‐Martín R , Salamone D
Published: December 2009

Contents

The aim of this study was to evaluate the potential of dehydroleucodine (DhL), a new drug isolated from a medicinal herb used in Argentina, for activation of bovine oocyte. Several DhL concentrations and exposure times after ionomycin (Io) treatment were tested. The optimal DhL treatment, found for parthenogenetic development, was employed to produce bovine embryos by intracytoplasmic sperm injection (ICSI) and somatic cell nuclear transfer (SCNT). The best parthenogenic embryo developments were observed with 5 μm Io for 4 min followed by 5 μm DhL concentration and after 3‐h exposure time (52.3% cleavage; 17.4% morulae; 7.3% blastocyst; n = 109). This treatment generated no significant differences with standard Io plus 6‐dimethylaminopurine (DMAP) treatment in preimplantation embryo development. In our conditions, the embryo development reached after ICSI and SCNT assisted by the DhL treatment did not differ in terms of cleavage and blastocyst development from activation with standard Io plus DMAP treatment (p > 0.05). In conclusion, DhL utilization to activate oocytes and induce development of parthenogenotes, ICSI‐embryos or SCNT‐embryos is reported here for first time.
The Missing Link Between the Theory and Empirics of Path Dependence: Conceptual Clarification, Testability Issue, and Methodological Implications
Authors: Vergne Jean‐Philippe , Durand Rodolphe
Published: December 2009

abstract

Path dependence is a central construct in organizational research, used to describe a mechanism that connects the past and the future in an abstract way. However, across institutional, technology, and strategy literatures, it remains unclear why path dependence sometimes occurs and sometimes not, why it sometimes lead to inefficient outcomes and sometimes not, how it differs from mere increasing returns, and how scholars can empirically support their claims on path dependence. Hence, path dependence is not yet a theory since it does not causally relate identified variables in a systematized manner. Instead, the existing literature tends to conflate path dependence as a process (i.e. history unfolding in a self‐reinforcing manner) and as an outcome (i.e. a persisting state of the world with specific properties, called ‘lock‐in’). This paper contributes theoretically and methodologically to tackling these issues by: (1) providing a formal definition of path dependence that disentangles process and outcome, and identifies the necessary conditions for path dependence; (2) distinguishing clearly between path dependence and other ‘history matters’ kinds of mechanisms; and (3) specifying the missing link between theoretical and empirical path dependence. In particular, we suggest moving away from historical case studies of supposedly path‐dependent processes to focus on more controlled research designs such as simulations, experiments, and counterfactual investigation.
When Experts Do Politics: Introducing Water Policy Reform in Brazil
Authors: GUTIÉRREZ RICARDO A.
Published: December 2009

This article brings to the fore the role of experts as policy agents by analyzing the water management reform in three Brazilian states. Seeking to contribute to the recent ideational turn in political science, it contends that in studying the emergence and implementation of new policy ideas, more attention must be paid to the role of experts as policy innovators and to the interplay between experts' knowledge brokerage and the influence of party politics. To examine the interaction between knowledge brokerage and party politics, comparative case studies were conducted to trace back throughout their different phases the reform processes of the Brazilian states of Ceará, Paraná, and Rio Grande do Sul. The purpose of this research design is to test the argument that experts are major reform agents and that what experts can effectively achieve depends on both the reform phase and the political context they find themselves in.
‘Amsterdam is Standing on Norway’ Part I: The Alchemy of Capital, Empire and Nature in the Diaspora of Silver, 1545–1648
Authors: MOORE JASON W.
Published: December 2009

In the first of two essays in this Journal, I seek to unify the historical geography of early modern ‘European expansion’ (Iberia and Latin America) with the environmental history of the ‘transition to capitalism’ (northwestern Europe). The expansion of Europe's overseas empires and the transitions to capitalism within Europe were differentiated moments within the geographical expansion of commodity production and exchange – what I call the commodity frontier. This essay is developed in two movements. Beginning with a conceptual and methodological recasting of the historical geography of the rise of capitalism, I offer an analytical narrative that follows the early modern diaspora of silver. This account follows the political ecology of silver production and trade from the Andes to Spain in Braudel's ‘second’ sixteenth century (c. 1545–1648). In highlighting the Ibero‐American moment of this process in the present essay, I contend that the spectacular reorganization of Andean space and the progressive dilapidation of Spain's real economy not only signified the rise and demise of a trans‐Atlantic, Iberian ecological regime, but also generated the historically necessary conditions for the unprecedented concentration of accumulation and commodity production in the capitalist North Atlantic in the centuries that followed.
Household Production and Capitalist Development in Contemporary Russia
Authors: COX TERRY
Published: December 2009

This essay reviews four recent books based on research on the development of capitalism and the position of household‐based farming in post‐Soviet Russia. Each of the books represents a different set of conceptual assumptions and is based on different methods of enquiry. It is argued that a problematic feature of much of the literature on this topic is that it begins from the assumption that successful capitalist development in Russian agriculture should be based on the development of small‐scale family farming. This tends to obscure the variety of forms of production that have emerged so far and the range of different relationships between them.
Balanced effect of PACAP and FasL on granule cell death during cerebellar development: a morphological, functional and behavioural characterization
Authors: Allais Aurélie , Burel Delphine , Roy Vincent , Arthaud Sébastien , Galas Ludovic , Isaac Emma Rachel , Desfeux Arnaud , Parent Bénédicte , Fournier Alain , Chapillon Pierre , Sherwood Nancy McKeown , Vaudry Hubert , Gonzalez Bruno José
Published: December 2009

J. Neurochem. (2010) 113, 329–340.

It is now established that the development of the CNS requires equilibrium between cell survival and apoptosis. Pituitary adenylate cyclase‐activating polypeptide (PACAP) exerts a powerful protective effect on cerebellar granule cells by inhibiting the caspase 3. In contrast, Fas ligand (FasL) plays an essential role during ontogenesis in eliminating supernumerary neurons by apoptosis. To determine if PACAP and FasL interact during cerebellar development, we characterized the effects of these factors on cerebellar morphogenesis and caspase 3 activity in PACAP+/+ and PACAP−/− mice. First, we demonstrated in vivo that PACAP is able to reverse the diminution of internal granule cell layer thickness induced by FasL in PACAP+/+ and PACAP−/− mice. Second, ex vivo and immunohistochemical studies revealed that interaction between FasL and PACAP occurs through the caspase 3 activity. Third, behavioural study showed a significant difference for the PACAP + FasL group in the righting reflex test at P8 which does not persist at P60. Finally, a time course study revealed that the pro‐apoptotic effect of FasL characterized at P8 was followed by a progressive compensatory mechanism in caspase 3 activity and bromodeoxyuridine incorporation. These data suggest that PACAP and FasL interact during cerebellar development to control apoptosis of granule cells and may affect some motor cerebellar functions.