bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of a Linear Measurement Tool in Virtual Reality for Assessment of Multimodality Imaging Data—A Phantom Study
Authors: Stephenson Natasha , Pushparajah Kuberan , Wheeler Gavin , Deng Shujie , Schnabel Julia A. , Simpson John M.
Published: November 2022

This study aimed to evaluate the accuracy and reliability of a virtual reality (VR) system line measurement tool using phantom data across three cardiac imaging modalities: three-dimensional echocardiography (3DE), computed tomography (CT) and magnetic resonance imaging (MRI). The same phantoms were also measured using industry-standard image visualisation software packages. Two participants performed blinded measurements on volume-rendered images of standard phantoms both in VR and on an industry-standard image visualisation platform. The intra- and interrater reliability of the VR measurement method was evaluated by intraclass correlation coefficient (ICC) and coefficient of variance (CV). Measurement accuracy was analysed using Bland–Altman and mean absolute percentage error (MAPE). VR measurements showed good intra- and interobserver reliability (ICC ≥ 0.99, p < 0.05; CV < 10%) across all imaging modalities. MAPE for VR measurements compared to ground truth were 1.6%, 1.6% and 7.7% in MRI, CT and 3DE datasets, respectively. Bland–Altman analysis demonstrated no systematic measurement bias in CT or MRI data in VR compared to ground truth. A small bias toward smaller measurements in 3DE data was seen in both VR (mean −0.52 mm [−0.16 to −0.88]) and the standard platform (mean −0.22 mm [−0.03 to −0.40]) when compared to ground truth. Limits of agreement for measurements across all modalities were similar in VR and standard software. This study has shown good measurement accuracy and reliability of VR in CT and MRI data with a higher MAPE for 3DE data. This may relate to the overall smaller measurement dimensions within the 3DE phantom. Further evaluation is required of all modalities for assessment of measurements <10 mm.
Childhood Trauma and Malevolent Creativity in Chinese College Students: Moderated Mediation by Psychological Resilience and Aggression
Authors: Li Wenfu , Zhang Linghui , Qin Zhilei , Chen Jingting , Liu Chuanxin
Published: November 2022

Although a previous study has shown that childhood trauma influences malevolent creativity, aggression and psychological resilience have been linked with childhood trauma and creativity. However, little is known about the complex correlations among these factors in Chinese college students. The present study aimed to investigate the mediating role of aggression and the moderating role of psychological resilience between childhood trauma and malevolent creativity. A total of 389 undergraduates were enrolled in this cross-sectional study. The moderated mediation model was conducted to explore whether aggression mediated the correlation between childhood trauma and malevolent creativity and whether psychological resilience moderated the indirect role of childhood trauma. The results showed that childhood trauma positively correlated with aggression and malevolent creativity and was negatively associated with psychological resilience. Aggression partly mediated the association of childhood trauma with malevolent creativity. Resilience moderated the indirect effect of the mediation model, such that the indirect effect of childhood trauma on malevolent creativity through aggression increased as the level of resilience increased. The study indicated that childhood trauma exposure is associated with malevolent creativity behavior, and aggression mediated this association. The level of psychological resilience differentiates the indirect paths of childhood trauma on malevolent creativity. These results have important implications for preventing and containing expressions of malevolent creativity.
The Artisanal Fishing Sector in the Spanish Mediterranean: A Sector with a Long History and an Uncertain Future
Authors: Márquez Escamilla Andrea , Herrera-Racionero Paloma , Pastor Gimeno José , Miret-Pastor Lluís
Published: November 2022

The fishing sector is facing major economic and environmental challenges worldwide. However, at least in developed countries, it is also undergoing a major social crisis. This research has tried to quantify and validate this subjective feeling of deep crisis, based on objective and quantitative data referring to the Spanish Mediterranean. Indeed, the results show alarming data. Different scattered databases have been analyzed and it has been exposed that in only 15 years the number of vessels, power and tonnage has decreased by about 40%; as for fishermen, their number has decreased by about 30%, also revealing a serious problem of generational replacement, since in only 10 years, the average age of fishermen has increased by 3 years. A simple linear regression model shows that this downward trend in the number of boats and fishermen will continue at least in the short term. The data obtained invite a deep reflection on the future or even the very survival of fishing in the Spanish Mediterranean in the medium term. This reflection is not limited to this specific area but can be extrapolated to many other fishing areas.
Numerical Investigation of Gas-Liquid Flow in a Multiphase Pump with Special Emphasis on the Effect of Tip Leakage Vortex on the Gas Flow Pattern
Authors: Xiao Yexiang , Gui Zhonghua , Li Xuesong , Shu Zekui , Shi Guangtai , Gu Chunwei
Published: November 2022

In this paper, the gas-liquid flow is comprehensively analyzed under different inlet gas void fractions, and the effect of tip leakage vortex (TLV) on the gas flow pattern in multiphase pumps is revealed. The results show that the gas flow pattern in an impeller is closely related to the centrifugal force, low-pressure region, and vortex motion. Most gas is present near the hub and suction surface of the blade as well as in the TLV. The two- and three-dimensional spatiotemporal evolution of the gas is presented, and the gas motion during the inception, development, and dissipation of TLV is revealed. It is reflected that the gas volume fraction is the highest at the TLV core and gradually weakens along the radial direction with the vortex core at the center. Additionally, the TLV energy dissipation is closely related to the gas and pressure difference, and strong energy dissipation occurs in the jet-wake flow.
Coastal Vulnerability Assessment for Future Sea Level Rise and a Comparative Study of Two Pocket Beaches in Seasonal Scale, Ios Island, Cyclades, Greece
Authors: Komi Apostolia , Petropoulos Alexandros , Evelpidou Niki , Poulos Serafeim , Kapsimalis Vasilios
Published: November 2022

The coastal zone may be considered as the location where the marine and land environments interact dynamically and coexist with human societies. Globally, natural and human systems are being severely threatened by the sea level rise related to climate change. The outcome between the dynamic relationship of coastal environments and marine processes, and the future sea level rise as predicted by scientific reports, is the vulnerability of coastal areas such as sandy beaches, pocket beaches and low-lying coastal areas. The current research aims to assess the coastal vulnerability of Ios Island, Cyclades, Greece for the next 100 years and to identify areas that are comparatively more vulnerable to future sea level changes. Moreover, the seasonal changes concerning sedimentological and morphological characteristics of two pocket beaches of Ios Island, Mylopotas and Magganari, are also examined. From the application of the Coastal Vulnerability Index, 92.37% of the total length of the coastline of Ios Island is characterized by a very low vulnerability as it consists of rocky shores and cliffs, while sandy and pocket beaches are characterized by a very high vulnerability. From the fieldworks and data processing, the seasonal changes mainly concern the seabed’s topography, the sediments’ texture of the collected sand samples, the foreshore and backshore topography, as well as seasonal shoreline displacement, using the Digital Shoreline Analysis System tool (DSAS).
Test Study on Vortex-Induced Vibration of Deep-Sea Riser under Bidirectional Shear Flow
Authors: Zhou Weiwei , Duan Menglan , Chen Rongqi , Wang Shisheng , Li Huiming
Published: November 2022

A model test was carried out to reveal the vortex-induced vibration characteristics of a deep-sea riser under bidirectional shear flow. Bandpass filtering and modal analysis were used to process the test strain data, and the amplitude and frequency response characteristics of the vortex-induced vibration of the riser in the bidirectional shear flow field were obtained. The results of the test data analysis show that the dominant frequency of the vortex-induced vibration of the riser model under bidirectional shear flow is locked in the natural frequency of the riser and does not increase with the increase in flow velocity, that the average resistance coefficient of the riser model has little change under different flow velocities because of the distribution characteristics of the “bidirectional shear” flow field, that there is an extreme value of the shear force in the middle of the riser model, and that the Strouhal number in the transverse direction of the vortex-induced vibration under bidirectional shear flow is less than the recommended value of the current vortex-induced vibration prediction software. The obtained results provide basic data for the prediction of vortex-induced vibration and research into the fatigue analysis method of a riser under an internal wave flow field.
Design of Dihedral Bows: A New Type of Developable Added Bulbous Bows—Experimental Results
Authors: Pérez-Arribas Francisco , Silva-Campillo Arturo , Díaz-Ojeda Héctor Rubén
Published: November 2022

This paper presents the design and the experimental results of a new type of developable added bulbous bow that has been designated as a dihedral bow. This type of bow is based on polyhedral bows that are used in small vessels, whose origin is traced to the 1990s. The bow is designed with a set of developable surfaces that are designed following previous methodology on surface design that considers material properties and can contain boundary curves. Two dihedral bow designs and their towing tank tests are presented in this work. A displacement and a semi-displacement hull were tested in two different loading conditions and for different Froude numbers. An important reduction of the effective power (PE) of the ships with the dihedral bow was observed during the experiments. There is a reduction of about 20% for the displacement hull and about 16% for the semi-displacement. The design methodology for the dihedral bows is presented in this paper together with experimental results on power, sink and trim. Dihedral bows are a good option for efficient small ship design, as well as larger ships.
Increased Temperature and Nitrogen Enrichment Inhibit the Growth of the Golden Tide Blooming Macroalgae Sargassum horneri in the Yellow Sea, China
Authors: Wu Hailong , Li Xuebing , Liu Yanhong , Wang Chuchu , Ji Chenkai , Xu Juntian
Published: November 2022

The golden tide, a large biomass bloom of the brown macroalgae Sargassum horneri, occurs yearly in the Yellow Sea, where it causes enormous economic and ecologic losses. To investigate the response of S. horneri to global warming and eutrophication, S. horneri was cultured under six conditions of varying temperature combinations (20 and 24 °C) and nitrogen levels (5, 30, and 300 μM). The growth, photosynthetic performance, pigment content, and contents of soluble protein were assessed. The growth of S. horneri followed an increasing trend with increasing N concentration at ambient temperature. Elevated temperatures had an inhibitory effect on growth and photosynthesis in S. horneri, which was further enhanced by eutrophication. This suggests that in the globally warming environment of the future, eutrophication may reduce the frequency and scale of gold tide outbreaks during the hot season.
Two New Free-Living Marine Nematode Species of the Genus Haliplectus Cobb, 1913 (Haliplectidae) from Mangroves of Shenzhen, Guangdong Province, China
Authors: Zhou Rengui , Chen Yuzhen , Shih Yijia , Guo Yuqing
Published: November 2022

This study presents two new free-living marine nematodes, Haliplectus major sp. nov. and Haliplectus futianisus sp. nov., from a mangrove reservation in Shenzhen, China. Haliplectus major sp. nov. can be distinguished by its total body length of 1615–1998 µm; a cuticle annulated with eight rows of cuticle pores; amphidial fovea at 9–12 µm from the anterior end; a bipartite basal bulb with striated valve plates in both sexes; five to seven closely spaced precloacal supplements in males; a didelphic reproductive system in females, with the vulva at 49.64–52.37% of body length; and sexual dimorphism in tail shape, arcuate-conoid and 2.3–3.2 times anal body diameter long in males and elongate-conid and 3.5–4.2 times anal body diameter long in females. Haliplectus futianisus sp. nov. is characterized by a total body length of 574–655 µm; a cuticle annulated with eight rows of cuticle pores; amphidial fovea at 8–11 µm from the anterior end; a monopartite basal bulb with unstriated valve plates in both sexes; an absence of precloacal supplements in males; and a didelphic reproductive system in females, with the vulva at 42.33–45.63% of body length. The identification was confirmed by analyzing morphological characteristic and 18S rRNA gene sequences. The maximum likelihood (ML) topology was regarded as morphological evidence of H. major sp. nov. and H. futianisus sp. nov. being two distinct species.
Pairwise-Comparison Based Semi-SPO Method for Ship Inspection Planning in Maritime Transportation
Authors: Yang Ying , Yan Ran , Wang Hans
Published: November 2022

Port state control (PSC) plays an important role in enhancing maritime safety and protecting the marine environment. Since the inspection resources are limited and the inspection process is costly and time-consuming, a critical issue for port states to guarantee inspection efficiency is to accurately select ships with a high risk for inspection. To address this issue, this study proposes three prediction models to predict the ship deficiency number and a ship selection optimization model based on the prediction results to target the riskiest ships for inspection. In addition to a linear regression model for ship deficiency number prediction solved by the least squares method, we establish two prediction models with the pairwise-comparison target based semi-“smart predict then optimize” (semi-SPO) method. Specifically, a linear programming model and a support vector machine (SVM) model are built and both have a loss function to minimize the sum of predicted ranking errors of each pair of ships regarding their deficiency numbers. We use the Hong Kong port as a case study, which shows that the SVM model based on the semi-SPO approach performs best among the three models with the least computation time and best ship selection decisions.
The Pathogenesis and Diagnosis of Pneumocystis jiroveci Pneumonia
Authors: Apostolopoulou Anna , Fishman Jay A.
Published: November 2022

Pneumocystis jiroveci remains an important fungal pathogen in immunocompromised hosts. The environmental reservoir remains unknown. Pneumonia (PJP) results from airborne transmission, including in nosocomial clusters, or with reactivation after an inadequately treated infection. Pneumocystis pneumonia most often occurs within 6 months of organ transplantation, with intensified or prolonged immunosuppression, notably with corticosteroids and following cytomegalovirus (CMV) infections. Infection may be recognized during recovery from neutropenia and lymphopenia. Invasive procedures may be required for early diagnosis and therapy. Despite being a well-established entity, aspects of the pathogenesis of PJP remain poorly understood. The goal of this review is to summarize the data on the pathogenesis of PJP, review the strengths and weaknesses of the pertinent diagnostic modalities, and discuss areas for future research.
Extracellularly Released Molecules by the Multidrug-Resistant Fungal Pathogens Belonging to the Scedosporium Genus: An Overview Focused on Their Ecological Significance and Pathogenic Relevance
Authors: Mello Thaís P. , Barcellos Iuri C. , Aor Ana Carolina , Branquinha Marta H. , Santos André L. S.
Published: November 2022

The multidrug-resistant species belonging to the Scedosporium genus are well recognized as saprophytic filamentous fungi found mainly in human impacted areas and that emerged as human pathogens in both immunocompetent and immunocompromised individuals. It is well recognized that some fungi are ubiquitous organisms that produce an enormous amount of extracellular molecules, including enzymes and secondary metabolites, as part of their basic physiology in order to satisfy their several biological processes. In this context, the molecules secreted by Scedosporium species are key weapons for successful colonization, nutrition and maintenance in both host and environmental sites. These biologically active released molecules have central relevance on fungal survival when colonizing ecological places contaminated with hydrocarbons, as well as during human infection, particularly contributing to the invasion/evasion of host cells and tissues, besides escaping from the cellular and humoral host immune responses. Based on these relevant premises, the present review compiled the published data reporting the main secreted molecules by Scedosporium species, which operate important physiopathological events associated with pathogenesis, diagnosis, antimicrobial activity and bioremediation of polluted environments.
Altered Cerebral Processing of Videos in Children with Motor Dysfunction Suggests Broad Embodiment of Perceptual Cognitive Functions
Authors: Ntoumanis Ioannis , Agranovich Olga , Shestakova Anna N. , Blagovechtchenski Evgeny , Koriakina Maria , Kadieva Dzerassa , Kopytin Grigory , Jääskeläinen Iiro P.
Published: November 2022

Embodied cognition theory suggests that motor dysfunctions affect cognition. We examined this hypothesis by inspecting whether cerebral processing of movies, featuring both goal-directed movements and content without humans, differ between children with congenital motor dysfunction and healthy controls. Electroencephalography was recorded from 23 healthy children and 23 children with limited or absent arm movement due to either arthrogryposis multiplex congenita or obstetric brachial plexus palsy. Each individual patient exhibited divergent neural responses, disclosed by significantly lower inter-subject correlation (ISC) of brain activity, during the videos compared to the healthy children. We failed to observe associations between this finding and the motor-related content of the various video scenes, suggesting that differences between the patients and controls reflect modulation of perceptual-cognitive processing of videos by upper-limb motor dysfunctions not limited to the watching-mirroring of motor actions. Thus, perceptual-cognitive processes in the brain seem to be more robustly embodied than has previously been thought.
Audiological Evidence of Frequent Hereditary Mild, Moderate and Moderate-to-Severe Hearing Loss
Authors: Markova Tatiana , Alekseeva Natalia , Lalayants Maria , Ryzhkova Oxana , Shatokhina Olga , Galeeva Nailya , Bliznetz Elena , Belov Oleg , Chibisova Svetlana , Polyakov Alexander , Tavartkiladze George
Published: November 2022

Congenital and early onset bilateral sensorineural hearing loss (SNHL) is mainly caused by mutations in numerous genes. The introduction of universal newborn hearing screening (UNHS) has increased the number of infants with mild, moderate, and moderate-to-severe sensorineural hearing loss (SNHL) detected in the first year of life. We aimed to evaluate the audiological features in patients with mild, moderate, and moderate-to-severe SNHL according to genotype. Audiological and genetic data were analyzed for 251 patients and their relatives with congenital bilateral mild, moderate, and moderate-to-severe SNHL. Hearing loss severity, audiogram profile, interaural symmetry, and dynamics of hearing thresholds were analyzed. In this case, 165 patients had GJB2 gene mutations, 30 patients were identified with STRC mutations, and 16 patients had pathogenic or likely pathogenic USH2A mutations. The presence of at least one GJB2 non-truncating variant in genotype led to less severe hearing impairment. The flat and gently sloping audiogram profiles were mostly revealed in all groups. The follow-up revealed the stability of hearing thresholds. GJB2, STRC, and USH2A pathogenic variants were detected in most patients in our cohort and were congenital in most cases.
Pitfalls and Rewards of Setting Up a Liquid Biopsy Approach for the Detection of Driver Mutations in Circulating Tumor DNAs: Our Institutional Experience
Authors: Chen Michelle , Jian Damon , Sidorov Maxim , Woo Rinette W. L. , Kim Angela , Stone David E. , Nazarian Ari , Nosrati Mehdi , Ice Ryan J. , de Semir David , Dar Altaf A. , Luštrik Roman , Kokošar Janez , Ausec Luka , Rowbotham Michael C. , Tranah Gregory J. , Kashani-Sabet Mohammed , Soroceanu Liliana , McAllister Sean D. , Desprez Pierre-Yves
Published: November 2022

We describe our institutional experience of developing a liquid biopsy approach using circulating tumor DNA (ctDNA) analysis for personalized medicine in cancer patients, focusing on the hurdles encountered during the multistep process in order to benefit other investigators wishing to set up this type of study in their institution. Blood samples were collected at the time of cancer surgery from 209 patients with one of nine different cancer types. Extracted tumor DNA and circulating cell-free DNA were sequenced using cancer-specific panels and the Illumina MiSeq machine. Almost half of the pairs investigated were uninformative, mostly because there was no trackable pathogenic mutation detected in the original tumor. The pairs with interpretable data corresponded to 107 patients. Analysis of 48 gene sequences common to both panels was performed and revealed that about 40% of these pairs contained at least one driver mutation detected in the DNA extracted from plasma. Here, we describe the choice of our overall approach, the selection of the cancer panels, and the difficulties encountered during the multistep process, including the use of several tumor types and in the data analysis. We also describe some case reports using longitudinal samples, illustrating the potential advantages and rewards in performing ctDNA sequencing to monitor tumor burden or guide treatment for cancer patients.
Neuroimaging-Based Brain Age Estimation: A Promising Personalized Biomarker in Neuropsychiatry
Authors: Sone Daichi , Beheshti Iman
Published: November 2022

It is now possible to estimate an individual’s brain age via brain scans and machine-learning models. This validated technique has opened up new avenues for addressing clinical questions in neurology, and, in this review, we summarize the many clinical applications of brain-age estimation in neuropsychiatry and general populations. We first provide an introduction to typical neuroimaging modalities, feature extraction methods, and machine-learning models that have been used to develop a brain-age estimation framework. We then focus on the significant findings of the brain-age estimation technique in the field of neuropsychiatry as well as the usefulness of the technique for addressing clinical questions in neuropsychiatry. These applications may contribute to more timely and targeted neuropsychiatric therapies. Last, we discuss the practical problems and challenges described in the literature and suggest some future research directions.
Vitamin D May Be Connected with Health-Related Quality of Life in Psoriasis Patients Treated with Biologics
Authors: Paliu Iulia-Alexandra , Ianosi Simona-Laura , Turcu-Stiolica Adina , Pisoschi Catalina-Gabriela , Predoi Luminita-Georgeta , Tica Andrei-Adrian
Published: November 2022

Suboptimal states of vitamin D may play a role in psoriasis evolution, but the interconnections have been studied over the past years with controversial results. Although a peerless therapy among moderate to severe types of psoriasis, the therapeutic effectiveness of biological therapy may vary unforeseeably between patients and leads to biologics switch. We conducted a pilot study in patients diagnosed with psoriasis and treated with biologics, the purpose of which was to explore the prevalence of suboptimal states of vitamin D, especially in the group of patients characterized by the failure of previous biologics, and to investigate the associations between vitamin D levels and psoriasis, regarding aspects such the severity of the disease and quality of life. Their current result of latent tuberculosis infection (LTBI) was also considered concerning a feasible relationship with vitamin D levels. From July to December 2021, 45 patients corresponding to our inclusion criteria were assessed. Variables such as Psoriasis Area and Severity Index (PASI) score and the Dermatology Life Quality Index (DLQI) score, as well as vitamin D serum concentrations and their LTBI result, were recorded for them. Lower serum concentrations of vitamin D were not more common in patients characterized by failure to previous biologics (p = 0.443), but we concluded a weak correlation between the DLQI score and vitamin D (rho = −0.345, p-value = 0.020), although a statistically insignificant result was obtained between vitamin D and the PASI score (rho = −0.280, p-value = 0.062), and with the LTBI result (rho = −0.053, p-value = 0.728). These results establish a connection between higher levels of vitamin D and a better outcome of psoriasis from the perspective of the patient’s quality of life, with no significant association with psoriasis severity and no significant prevalence of suboptimal states among patients that failed previous biologics compared to those with a continuously good response.
Corporate Governance and Financial Stability: The Case of Commercial Banks in Vietnam
Authors: Nguyen Thi Nhu Quynh , Nguyen Duc Trung , Le Hoang Anh , Le Dinh Luan
Published: November 2022

Bank stability is a goal that bank managers aim for in addition to the goal of maximizing shareholder value. To achieve this goal, commercial banks have applied various solutions, including corporate governance because corporate governance plays an important role in the business activities of an enterprise in general as well as in that of a commercial bank in particular. The purpose of this paper is to investigate the impact of corporate governance on the stabilities of Vietnamese commercial banks in the period from 2009 to 2020. Using hand-collected data from 25 commercial banks in Vietnam, by system GMM estimation and the Bayesian Mixed-Effects approach, the paper identifies the characteristics of corporate governance affecting bank stability. Board size, women board members, and board members’ education have a positive impact, and dependent board and foreign board members have a negative impact on bank stability. Our findings show important evidence for an emerging country, such as Vietnam. From the empirical results, the authors suggest several recommendations to maintain and enhance bank stability in the future time.
Time Dependence of CAPM Betas on the Choice of Interval Frequency and Return Timeframes: Is There an Optimum?
Authors: Agrrawal Pankaj , Gilbert Faye W. , Harkins Jason
Published: November 2022

The traditional CAPM beta is almost exclusively calculated over a return period that spans a window length of 60-months, at one-month return frequencies. It is one of the most utilized models in the asset management industry to assess systematic risk. Yet there is limited evidence to suggest that these estimation parameters are optimal. Utilizing data between January 2000 and December 2021 for the Russell 1000 index, we test daily, weekly, and monthly beta estimations to calculate tracking errors (TE) for the use of these betas in predicting subsequent performance over daily, weekly, and monthly timeframes. We identify that daily CAPM betas are best for predicting subsequent period daily returns and that weekly CAPM betas are strongly correlated with forward weekly and monthly period returns. Leveraging the significant advances in computing resources and the increasing utilization of high frequency trading strategies, we argue that additional window length and return interval-based CAPM betas should be calculated for estimating the systematic risk embedded in diversified portfolios.
Behavioural Impact of Captive Management Changes in Three Species of Testudinidae
Authors: Turner Jessica T. , Whittaker Alexandra L. , McLelland David
Published: November 2022

Reptile behaviour and welfare are understudied in comparison with mammals. In this study, behavioural data on three species (Astrochelys radiata, Stigmochelys pardalis, Aldabrachelys gigantea) of tortoises were recorded before and after an environmental change which was anticipated to be positive in nature. The environmental changes differed for each population, but included a substantial increase in enclosure size, the addition of substrate material, and a change in handling procedure. A tortoise-specific ethogram was created to standardise data collection. Focal behaviour sampling was used to collect behavioural data. Changes in the duration of performance of co-occupant interaction and object interaction in the leopard (Stigmochelys pardalis) and Aldabra (Aldabrachelys gigantea) tortoises were observed following the environmental changes. The Shannon–Weiner diversity index did not yield a significant increase after the changes but had a numerical increase which was relatively greater for the leopard tortoise group, which had experienced the greatest environmental change. The leopard tortoises also demonstrated changes in a greater number of behaviours compared to the other species, and this was sustained over the study period. However, this included a behaviour indicative of negative affect: aggression. Whilst we are unable to conclude that welfare was improved by the management changes, there are suggestions that behavioural diversity increased, and some promotion of positive social behaviours occurred.
Progress of Study on Interception of Soil Mulching with an Insight into Karst Soil Leakage Control: A Review
Authors: Wu Chenxu , Xiong Kangning , Luo Ding , Gu Xing
Published: November 2022

Soil erosion is a global issue of great concern, especially in karst areas with special environments, where subsurface soil leakage is closely related to soil erosion, which has become a key factor limiting agricultural development. To explore how to improve soil erosion in karst areas to enhance soil quality and maintain the sustainable use of the land in the long term, a total of 176 studies on the interception characteristics of soil mulching and erosion management were reviewed using a systematic review approach, through the WoS and CNKI databases. Firstly, quantitative analysis was conducted in terms of the annual volume, content and countries of the published literature. Secondly, from four aspects (theoretical research, mechanism research, technology research and technical demonstration), the main progress and landmark achievements of soil mulching interception and erosion management were classified. It is shown that the interception characteristics of soil mulching can produce an effective blockage for soil leakage in karst areas. Based on the global classification, compared to synthetic materials, natural materials have received more attention. We propose five key scientific questions that still need to be addressed. This review explores the insightful role of soil mulching for karst soil leakage management and aims to provide theoretical support for future research on sustainable land development in karst areas.
Unsustainable Urban Development Based on Temporary Workers: A Study on the Changes of Immigration in Macau between 1992 and 2019
Authors: Zhou Bo , Jiang Lei
Published: November 2022

Macau’s urban development model has many unique characteristics, including expansion of the city through sea reclamation, increasing population mainly through immigration, and economic development driven by the gaming industry. Based on data from the Macau Statistics and Census Service, this study uses the Error Correction representation of the Autoregressive Distributed Lag model (ARDL-ECM) to analyze the impact of urban development on the trends of immigration and labor migration in Macau between 1992 and 2019. Results show that both land area and wage level have positive effects on the number of migrant workers and negative effects on the number of immigrants, indicating that Macau is over-dependent on short-term migrant workers. Macau’s land and human resources are tilted towards the gaming industry, resulting in a decreasing living environment and resident carrying capacity as the city develops. Therefore, this paper suggests that Macau should reduce the cost of city expansion and improve economic diversity through strengthening cooperation with neighboring mainland cities, hence sparing resources to absorb non-local talent and ensuring sustainable urban development.
Multi-Agent-Based Model for the Urban Macro-Level Impact Factors of Building Energy Consumption on Different Types of Land
Authors: Song Shiyi , Leng Hong , Guo Ran
Published: November 2022

Urban researchers pay more and more attention to building energy consumption from different perspectives to obtain the results of urban overall energy conservation. The research at the micro level has yielded abundant accomplishments, but the macro-level research that can support urban planning decision making is still in the exploration stage. In this study, a multi-agent-based model, including the main panel, building agent, resident agent, and household appliance agent, is established by using Anylogic software. The model integrates Harbin urban macro-level impact factors of building energy consumption by designing and linking three sub-models: an urban morphology sub-model, climate sub-model, and energy use behavior sub-model. In the end, this study explored the building energy-saving potential of different types of land under the impact of variable factors through urban morphology and climate simulation scenarios and discussed the related energy-saving strategies. Findings and suggestions include: (1) The impact of urban morphology on overall urban building energy consumption is mainly reflected in residential and commercial land. The land development intensity (building density, floor area ratio, and building height) control and the coordination of land type layout and configuration can help to reduce the building energy consumption. (2) The energy-saving potential of residential land is more evident under climate impact, and ecological means should be used to adjust the climate to reduce the building energy consumption on different lands. (3) From the methodology perspective, this model can well realize the integration of multiple impact factors at the macro-level of the city and the dynamic simulation of energy consumption. The research results are expected to provide quantitative support for creating a sustainable built environment for the city.
Demographic Change and the Urban–Rural Divide: Understanding the Role of Density and Agglomeration in Fertility Transitions
Authors: Nickayin Samaneh Sadat , Nosova Bogdana , Turco Rosario , Giacalone Massimiliano , Salvati Luca
Published: November 2022

Assuming fertility variations across urban–rural gradients, our study focuses on the traditional polarization in urban and rural fertility, offering a refined interpretation of demographic processes associated with population density. More specifically, we tested the intimate relationship between local fertility and population density, comparing the outcomes of a classical urban–rural model (reflecting a linear relationship between the two variables) with those of a more complex quadratic model (implying the so-called ‘suburban fertility hypothesis’) in Greece. We considered fertility dynamics in three districts (urban, suburban, and rural) of 51 Greek prefectures for the last two decades (2000–2009 and 2010–2019) and controlled for the diverging impact of local contexts at different population density levels. Taken as a measure of ‘maturity’ of regional systems, urban fertility surpassed rural fertility in almost all prefectures of Greece. An additional sign of maturity in metropolitan systems indicates that suburban birth rates are higher than urban birth rates in prefectures with high population density (Athens, Thessaloniki, Heraklion, and Patras). The regression outcomes document a specific response of fertility to regional development, evidencing a spatially differentiated shift from classical urban–rural disparities toward a more complex model with the emergence of suburban poles. From this perspective, fertility divides reflect the evolutions of socioeconomic forces (more or less rapidly) along the urban gradient.
Phylogenetic Relationships and Disturbance Explain the Resistance of Different Habitats to Plant Invasions
Authors: Guo Chaodan , Zhao Caiyun , Li Feifei , Huang Jianfeng
Published: November 2022

Invasive alien plants have invaded various habitats, posing a threat to biodiversity. Several hypotheses have been proposed to explain the mechanisms of invasion, but few studies have considered the characteristics of the invaded communities and the effects of human interference in the invasion. In this study, we compared the invasibility of three different habitats: abandoned land, eucalyptus plantations, and natural secondary forests. We explored the effects of species diversity, phylogenetic diversity, and disturbance factors on the invasibility of different habitats. The results showed that the invasibility of abandoned land was the highest and the invasibility of the natural secondary forest was the lowest. Phylogenetic indicators affected the invasibility of abandoned land and eucalyptus plantations, and disturbance factors affected the invasibility of all three habitats, while the characteristics of the invaded communities had a weak impact. Our research provided supporting evidence for Darwin’s naturalization hypothesis and his disturbance hypothesis but found no relationship between biotic resistance and invasibility. This study indicated that the differences among habitats should be considered when we prove Darwin’s naturalization hypothesis in nature reserves.
Recombinant Globular Domain of TcpA Pilin from Vibrio cholerae El Tor: Recovery from Inclusion Bodies and Structural Characterization
Authors: Marchenkov Victor , Dubovitskya Elena , Kotova Nina , Tuchkov Igor , Smirnova Nina , Marchenko Natalia , Surin Alexey , Filimonov Vladimir , Semisotnov Gennady
Published: November 2022

The production of recombinant proteins in Escherichia coli cells is often hampered by aggregation of newly synthesized proteins and formation of inclusion bodies. Here we propose the use of transverse urea gradient electrophoresis (TUGE) in testing the capability of folding of a recombinant protein from inclusion bodies dissolved in urea. A plasmid encoding the amino acid sequence 55–224 of TcpA pilin (C-terminal globular domain: TcpA-C) from Vibrio cholerae El Tor enlarged by a His-tag on its N-terminus was expressed in E. coli cells. The major fraction (about 90%) of the target polypeptide was detected in cell debris. The polypeptide was isolated from the soluble fraction and recovered from inclusion bodies after their urea treatment. Some structural properties of the polypeptide from each sample proved identical. The refolding protocol was developed on the basis of TUGE data and successfully used for the protein large-scale recovery from inclusion bodies. Spectral, hydrodynamic, and thermodynamic characteristics of the recombinant TcpA recovered from inclusion bodies indicate the presence of a globular conformation with a pronounced secondary structure and a rigid tertiary structure, which is promising for the design of immunodiagnostics preparations aimed to assess the pilin level in different strains of V. cholerae and to develop cholera vaccines.
Current Concepts and Future Applications of Non-Invasive Functional and Anatomical Evaluation of Coronary Artery Disease
Authors: Oikonomou Evangelos , Theofilis Panagiotis , Lampsas Stamatios , Katsarou Ourania , Kalogeras Konstantinos , Marinos Georgios , Tsatsaragkou Aikaterini , Anastasiou Artemis , Lysandrou Antonios , Gounaridi Maria-Ioanna , Gialamas Ioannis , Vavuranakis Michael-Andrew , Tousoulis Dimitris , Vavuranakis Manolis , Siasos Gerasimos
Published: November 2022

Over the last decades, significant advances have been achieved in the treatment of coronary artery disease (CAD). Proper non-invasive diagnosis and appropriate management based on functional information and the extension of ischemia or viability remain the cornerstone in the fight against adverse CAD events. Stress echocardiography and single photon emission computed tomography are often used for the evaluation of ischemia. Advancements in non-invasive imaging modalities such as computed tomography (CT) coronary angiography and cardiac magnetic resonance imaging (MRI) have not only allowed non-invasive imaging of coronary artery lumen but also provide additional functional information. Other characteristics regarding the plaque morphology can be further evaluated with the latest modalities achieving a morpho-functional evaluation of CAD. Advances in the utilization of positron emission tomography (PET), as well as software advancements especially regarding cardiac CT, may provide additional prognostic information to a more evidence-based treatment decision. Since the armamentarium on non-invasive imaging modalities has evolved, the knowledge of the capabilities and limitations of each imaging modality should be evaluated in a case-by-case basis to achieve the best diagnosis and treatment decision. In this review article, we present the most recent advances in the noninvasive anatomical and functional evaluation of CAD.
MICA Polymorphism and Genetic Predisposition to T1D in Jordanian Patients: A Case-Control Study
Authors: Jarrar Wassan , Khdair Sawsan I. , Khudeir Feras A.
Published: November 2022

Type 1 diabetes (T1D) is an autoimmune disorder whose etiology includes genetic and environmental factors. The non-classical Major Histocompatibility Complex (MHC) class I chain-related gene A (MICA) gene has been associated with increased susceptibility to T1D as the interaction of MICA to the Natural Killer Group 2D (NK2GD) receptors found on the cell surface of natural killer (NK) cells and T cells is responsible for inducing immune responses. MICA polymorphisms were reported in association with T1D among different ethnic groups. However, data from different populations revealed conflicting results, so the association of MICA polymorphisms with predisposition to T1D remains uncertain. The aim of this sequencing-based study was to identify, for the first time, the possible MICA alleles and/or genotypes that could be associated with T1D susceptibility in the Jordanian population. Polymorphisms in exons 2–4 and the short tandem repeats (STR) in exon 5 of the highly polymorphic MICA gene were analyzed. No evidence for association between T1D and MICA alleles/genotypes was found in this study, except for the MICA*011 allele which was found to be negatively associated with T1D (p = 0.023, OR = 0.125). In conclusion, MICA polymorphisms seem not to be associated with increasing T1D susceptibility in Jordanian patients.
Application of Potassium after Waterlogging Improves Quality and Productivity of Soybean Seeds
Authors: Mamun Muhammad Abdullah Al , Julekha Julekha , Sarker Umakanta , Mannan Muhammad Abdul , Rahman Mohammad Mizanur , Karim Md. Abdul , Ercisli Sezai , Marc Romina Alina , Golokhvast Kirill S.
Published: November 2022

Potassium (K) improves the stress tolerance of crop plants, which varies on the timing of K application and crop varieties. Soybean is a promising crop that can easily fit with the cropping pattern during kharif I season, when water logging occurs due to sudden rain. Therefore, an experiment was conducted to determine the effect of K management on the productivity and seed quality of soybean under normal and waterlogged conditions. The treatments comprised three factors, namely soybean genotypes (BU Soybean-1 and BU Soybean-2), waterlogging (WL) (control and WL for 4 days at the flowering stage (FS)), and K application (full dose as basal and 50% as basal +50% as top dress after termination of the flooding). The trial was laid out in a randomized complete block design with three replications. Findings revealed that BU Soybean-1 produced a higher number of pods and seeds pod−1 under control conditions with basal application of K. On the other hand, BU Soybean-2 produced taller plants and heavier grain, improving grain and straw yield under WL conditions when K was top dressed. The varieties absorbed a higher amount of nitrogen, phosphorus, and potassium under control conditions compared to WL when K was top dressed. Similarly, the seed protein content of both varieties was higher in the control condition with a top dressing of K. However, a higher percentage of seed germination was obtained from BU Soybean-2 in the control condition with a top dressing of K. Further, more electrical conductivity and more mean germination time were recorded in the case of BU Soybean-2 under WL with the basal application of K. Split application of 50% of recommended K fertilizer after the recession of flood water could be suggested for improved grain yield in flood-affected soybean growing areas.
Fungal Biodeterioration of a Historical Manuscript Dating Back to the 14th Century: An Insight into Various Fungal Strains and Their Enzymatic Activities
Authors: Abdel-Maksoud Gomaa , Abdel-Nasser Mahmoud , Sultan Mahmoud H. , Eid Ahmed M. , Alotaibi Saad H. , Hassan Saad El-Din , Fouda Amr
Published: November 2022

This study aims to assess the deterioration aspects of a historical manuscript dating back to the 14th century that was deposited in the Library of the Arabic Language Academy, Cairo, Egypt. The study aims at the exploration of the role of various fungal strains that had colonized this deteriorated manuscript in its biodeterioration through their efficacy in the secretion of various hydrolytic enzymes. To evaluate the deterioration, various techniques, including visual inspection, attenuated total reflectance Fourier transform infrared (ATR-FTIR), scanning electron microscopy (SEM), X-Ray diffraction analysis (XRD), color change, and pH value, were utilized. The fungal strains linked to the historical document were isolated, identified, and evaluated for their deterioration activities. The findings demonstrate that the manuscript exhibits a variety of deterioration signs including color change, brittleness and weakness, erosion, and removal of the grain surface pattern in leather binding. According to the ATR-FTIR, the chemical composition of the historical paper and leather underwent some alterations. The historical paper has a lower level of cellulose crystallinity than the control sample. Penicillium chrysogenum (two isolates), P. citrinum (four isolates), Aspergillus ustus (three isolates), A. terreus (two isolates), A. chinensis (one isolate), Paecilomyces sp. (one isolate), and Induratia sp. (one isolate) were among the fourteen fungal strains identified as being associated with the historical manuscript. These fungal strains produced several hydrolytic enzymes with high activity, such as cellulase, amylase, gelatinase, and pectinase, which play a key role in biodegradation.