bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of Radiation Resistance of WO3 Microparticles under Irradiation with Heavy Kr15+ and Xe22+ Ions
Authors: Kadyrzhanov Dauren B. , Kozlovskiy Artem L. , Zdorovets Maxim V. , Khametova Ainagul A. , Shlimas Dmitriy I.
Published: August 2022

In this work, we consider the effect of irradiation with heavy Kr15+ and Xe22+ ions on the change in the structural and strength properties of WO3 microparticles, which are among the candidates for inert matrix materials. Irradiation with heavy Kr15+ and Xe22+ ions was chosen to determine the possibility of simulation of radiation damage comparable to the impact of fission fragments. During the studies, it was found that the main changes in the structural properties with an increase in the irradiation fluence are associated with the crystal lattice deformation and its anisotropic distortion, which is most pronounced during irradiation with heavy Kr15+ ions. An assessment of the gaseous swelling effect due to the radiation damage accumulation showed that a change in the ion type during irradiation leads to an increase in the swelling value by more than 8–10%. Results of strength changes showed that the most intense decrease in the hardness of the near-surface layer is observed when the fluence reaches more than 1012 ion/cm2, which is typical for the effect of overlapping radiation damage in the material. The dependences obtained for the change in structural and strength properties can later be used to evaluate the effectiveness of the use of refractory oxide materials for their use in the creation of inert matrices of nuclear fuel.
Green-Graphene Protective Overlayer on Optical Microfibers: Prolongs the Device Lifetime
Authors: Novikova Anastasia , Katiyi Aviad , Halstuch Aviran , Karabchevsky Alina
Published: August 2022

Optical microfibers find new applications in various fields of industry, which in turn require wear resistance, environmental friendliness and ease of use. However, optical microfibers are fragile. Here we report a new method to prolong the microfiber lifetime by modifying its surface with green-extracted graphene overlayers. Graphene films were obtained by dispergation of shungite mineral samples in an aqueous medium. For this, we tapered optical fibers and sculptured them with graphene films mixed with gold nanoparticles. We observed that due to the surface modification the lifetime and survivability of the microfiber increased 5 times, as compared to the bare microfiber. The embedded gold nanoparticles can also be utilized for enhanced sensitivity and other applications.
Zn-Loaded and Calcium Phosphate-Coated Degradable Silica Nanoparticles Can Effectively Promote Osteogenesis in Human Mesenchymal Stem Cells
Authors: Sutthavas Pichaporn , Schumacher Matthias , Zheng Kai , Habibović Pamela , Boccaccini Aldo Roberto , van Rijt Sabine
Published: August 2022

Nanoparticles such as mesoporous bioactive glasses (MBGs) and mesoporous silica nanoparticles (MSN) are promising for use in bone regeneration applications due to their inherent bioactivity. Doping silica nanoparticles with bioinorganic ions could further enhance their biological performance. For example, zinc (Zn) is often used as an additive because it plays an important role in bone formation and development. Local delivery and dose control are important aspects of its therapeutic application. In this work, we investigated how Zn incorporation in MSN and MBG nanoparticles impacts their ability to promote human mesenchymal stem cell (hMSC) osteogenesis and mineralization in vitro. Zn ions were incorporated in three different ways; within the matrix, on the surface or in the mesopores. The nanoparticles were further coated with a calcium phosphate (CaP) layer to allow pH-responsive delivery of the ions. We demonstrate that the Zn incorporation amount and ion release profile affect the nanoparticle’s ability to stimulate osteogenesis in hMSCs. Specifically, we show that the nanoparticles that contain rapid Zn release profiles and a degradable silica matrix were most effective in inducing hMSC differentiation. Moreover, cells cultured in the presence of nanoparticle-containing media resulted in the highest induction of alkaline phosphate (ALP) activity, followed by culturing hMSC on nanoparticles immobilized on the surface as films. Exposure to nanoparticle-conditioned media did not increase ALP activity in hMSCs. In summary, Zn incorporation mode and nanoparticle application play an important role in determining the bioactivity of ion-doped silica nanoparticles.
Fe3O4@SiO2@VAN Nanoadsorbent Followed by GC-MS for the Determination of Polycyclic Aromatic Hydrocarbons at Ultra-Trace Levels in Environmental Water Samples
Authors: Tian Yu , Xu Zhigang , Liu Zhimin , Si Xiaoxi , Zhang Fengmei , Jiang Wei
Published: August 2022

In the present study, silica-coated magnetic nanoparticles functionalized with vancomycin (Fe3O4@SiO2@VAN) were synthesized. The Fe3O4@SiO2@VAN nanocomposite was used as a sorbent for the magnetic solid-phase extraction (MSPE) of polycyclic aromatic hydrocarbons (PAHs) from environmental water, followed by GC-MS. The nanocomposite was characterized by Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, and nitrogen sorption. Various experimental parameters were optimized, including extraction condition and desorption condition. Results show that Fe3O4@SiO2@VAN combined the advantages of nanomaterials and magnetic separation technology, showing excellent dispersibility and high selectivity for PAHs in environmental water sample. Under the optimal extraction conditions, an analytical method was established with the sensitive limit of detection (LOD) of 0.03–0.16 μg L−1. The method was successfully applied for the analysis of environmental water samples. The relative standard deviations (%) were in the range of 0.50–12.82%, and the extraction recovery (%) was in the range of 82.48% and 116.32%. MSPE-coupled gas chromatography–mass spectrometry quantification of PAHs is an accurate and repeatable method for the monitoring of PAH accumulation in environmental water samples. It also provides an effective strategy for the tracing and quantification of other environmental pollutants in complex samples.
Gut Microbiota Dysbiosis after Traumatic Brain Injury Contributes to Persistent Microglial Activation Associated with Upregulated Lyz2 and Shifted Tryptophan Metabolic Phenotype
Authors: Zheng Zhipeng , Wang Shuai , Wu Chenghao , Cao Yang , Gu Qiao , Zhu Ying , Zhang Wei , Hu Wei
Published: August 2022

Traumatic brain injury (TBI) is a common cause of disability and mortality, affecting millions of people every year. The neuroinflammation and immune response post-TBI initially have neuroprotective and reparative effects, but prolonged neuroinflammation leads to secondary injury and increases the risk of chronic neurodegenerative diseases. Persistent microglial activation plays a critical role in chronic neuroinflammation post-TBI. Given the bidirectional communication along the brain–gut axis, it is plausible to suppose that gut microbiota dysbiosis post-TBI influences microglial activation. In the present study, hippocampal microglial activation was observed at 7 days and 28 days post-TBI. However, in TBI mice with a depletion of gut microbiota, microglia were activated at 7 days post-TBI, but not at 28 days post-TBI, indicating that gut microbiota contributes to the long-term activation of microglia post-TBI. In addition, in conventional mice colonized by the gut microbiota of TBI mice using fecal microbiota transplant (FMT), microglial activation was observed at 28 days post-TBI, but not at 7 days post-TBI, supporting the role of gut microbiota dysbiosis in persistent microglial activation post-TBI. The RNA sequencing of the hippocampus identified a microglial activation gene, Lyz2, which kept upregulation post-TBI. This persistent upregulation was inhibited by oral antibiotics and partly induced by FMT. 16s rRNA gene sequencing showed that the composition and function of gut microbiota shifted over time post-TBI with progressive dysbiosis, and untargeted metabolomics profiling revealed that the tryptophan metabolic phenotype was differently reshaped at 7 days and 28 days post-TBI, which may play a role in the persistent upregulation of Lyz2 and the activation of microglia. This study implicates that gut microbiota and Lyz2 are potential targets for the development of novel strategies to address persistent microglial activation and chronic neuroinflammation post-TBI, and further investigations are warranted to elucidate the specific mechanism.
Association between Adherence to the Mediterranean Diet and Anthropometric and Health Variables in College-Aged Males
Authors: Prieto-González Pablo , Sánchez-Infante Jorge , Fernández-Galván Luis Miguel
Published: August 2022

The present study aimed to verify the association between adherence to the Mediterranean diet (MD) and anthropometric and health variables. Four-hundred-and-ninety-five college-aged males aged 18–25 participated in this cross-sectional research. The KIMED (Mediterranean Diet Quality Index for children and adolescents) was used to assess the adherence to MD. The following variables were also assessed: body mass (BM), height (HE), body mass index (BMI), body fat percentage (%FAT), lean mass (LEAN), abdominal girth (AG), waist-to-hip ratio (WHR), oxygen saturation (SPO2), systolic blood pressure (SBP), diastolic blood pressure (DBP), double product (DP), and fasting blood glucose (GLU). The results showed that adherence to MD presented a strong negative correlation with most of the anthropometric parameters (BM: r = −0.571; BMI: r = −0.614; %FAT: r = −0.558; and AG: r = −0.564), a moderate or weak correlation with most of the health variables (GLU: r = −0.407; SBP: r = −0.238; DBP: r = −0.217, and DP: r = −0.265) and LEAN (r = −0.497), and a very weak correlation with WHR (r = −0.090). Many anthropometric parameters (BM, BMI, %FAT, LEAN, AG, WHR) present significant correlations with health variables (SBP, DBP, DP, and GLU). We conclude that greater adherence to Mediterranean diet is associated with healthier values of the selected anthropometric and health parameters. Since most of the anthropometric and health parameters present significant correlations among themselves, this finding could be useful in medical diagnosis, health monitoring, and risk detection. Based on the level of adherence to Mediterranean diet and the KIDMED found in the present study, and considering the prevalence of obesity in the Middle East, it is imperative to implement nutritional interventions with the target population of this research to prevent nutrition-related diseases and promote public health.
Intraamniotic Administration (Gallus gallus) of Genistein Alters Mineral Transport, Intestinal Morphology, and Gut Microbiota
Authors: Cheng Jacquelyn , Kolba Nikolai , Sisser Philip , Turjeman Sondra , Even Carmel , Koren Omry , Tako Elad
Published: August 2022

Genistein is an isoflavone naturally present in numerous staple food crops, such as soybeans and chickpeas. This study utilized the Gallus gallus intraamniotic administration procedure to assess genistein administration effects on trace mineral status, brush border membrane (BBM) functionality, intestinal morphology, and intestinal microbiome in vivo. Eggs were divided into five groups with 1 mL injection of the following treatments: no-injection, DI H2O, 5% inulin, and 1.25% and 2.5% genistein (n = 8 per group). Upon hatch, blood, cecum, small intestine, and liver were collected for assessment of hemoglobin, intestinal microflora alterations, intestinal morphometric assessment, and mRNA gene expression of relevant iron and zinc transporter proteins, respectively. This study demonstrated that intraamniotic administration of 2.5% genistein increased villus surface area, number of acidic goblet cells, and hemoglobin. Additionally, genistein exposure downregulated duodenal cytochrome B (DcytB) and upregulated hepcidin expression. Further, genistein exposure positively altered the composition and function of the intestinal microbiota. Our results suggest a physiological role for genistein administration in improving mineral status, favorably altering BBM functionality and development, positively modulating the intestinal microbiome, as well as improving physiological status.
Pretreatment Glasgow Prognostic Score Correlated with Serum Histidine Level and Three-Year Mortality of Patients with Locally Advanced Head and Neck Squamous Cell Carcinoma and Optimal Performance Status
Authors: Yeh Kun-Yun , Wang Chao-Hung , Ling Hang Huong , Peng Chia-Lin , Chen Zih-Syuan , Hsia Simon
Published: August 2022

Few prospective cohort trials have investigted the effect of pretreatment nutritional and inflammatory status on the clinical outcome of patients with cancer and optimal performance status and assessed the interplay between nutrition, inflammation, body composition, and circulating metabolites before treatment. Here, 50 patients with locally advanced head and neck squamous cell carcinoma (LAHNSCC) and Eastern Cooperative Oncology Group performance status (ECOG PS) ≤ 2 were prospectively recruited along with 43 healthy participants. Before concurrent chemoradiotherapy, compared with healthy controls, the cancer group showed lower levels of histidine, leucine, and phenylalanine and had low values in anthropometric and body composition measurements; however, the group displayed higher ornithine levels, more malnutrition, and severe inflammation. Pretreatment advanced Glasgow prognostic score (1 and 2) status was the sole prognostic factor for 3-year mortality rate and was associated with age and serum histidine levels in patients with cancer. Thus, even at the same tumor stage and ECOG PS, patients with LAHNSCC, poor nutrition, and high inflammation severity at baseline may have inferior survival outcomes than those with adequate nutrition and low inflammation severity. Assessment of pretreatment nutritional and inflammatory status should be included in the enrollment criteria in future studies.
In Vitro Digestibility and Bioaccessibility of Nutrients and Non-Nutrients Composing Extruded Brewers’ Spent Grain
Authors: Gutierrez-Barrutia Maria Belen , Cozzano Sonia , Arcia Patricia , del Castillo Maria Dolores
Published: August 2022

This study aimed to evaluate the effect of the extrusion process on the bioaccessibility of brewers’ spent grain (BSG) nutrients (carbohydrates and proteins) and non-nutrients (bioactive compounds). BSG and extruded BSG (EBSG) were digested in vitro simulating human oral-gastro-intestinal digestion and colonic fermentation. The duodenal bioaccessibility of glucose, amino acids and phenolic compounds was analyzed. The fermentability of the dietary fiber was assessed by analysis of short-chain fatty acids. Additionally, assessment of the bioaccessibility of phenolic compounds after colonic fermentation was undertaken. The antioxidant, anti-inflammatory and antidiabetic properties of the bioaccessible compounds were studied. Extrusion caused no change in the digestibility of gluten and glucose bioaccessibility (p > 0.05). Moreover, the bioaccessibility of amino acids and phenolic compounds significantly increased (p < 0.05) due to extrusion. However, higher short-chain fatty acid content was formed in colonic fermentation of BSG (p < 0.05) compared to EBSG. The latter inhibited intracellular ROS formation in IEC-6 cells and showed anti-inflammatory properties in RAW264.7 cells. With respect to antidiabetic properties, glucose absorption was lower, and the inhibition of carbohydrases higher (p < 0.05), in the presence of EBSG compared to BSG. The effects of EBSG and BSG digests on glucose transporters were not significantly different (p > 0.05). In conclusion, extrusion positively affected the nutritional value and health-promoting properties of BSG.
Effect of 24-Week, Late-Evening Ingestion of a Calcium-Fortified, Milk-Based Protein Matrix on Biomarkers of Bone Metabolism and Site-Specific Bone Mineral Density in Postmenopausal Women with Osteopenia
Authors: Norton Catherine , Hettiarachchi Manjula , Cooke Rachel , Kozior Marta , Kontro Hilkka , Daniel Rosemary , Jakeman Philip
Published: August 2022

Dietary calcium intake is a modifiable, lifestyle factor that can affect bone health and the risk of fracture. The diurnal rhythm of bone remodelling suggests nocturnal dietary intervention to be most effective. This study investigated the effect of daily, bed-time ingestion of a calcium-fortified, milk-derived protein matrix (MBPM) or control (CON), for 24 weeks, on serum biomarkers of bone resorption (C-terminal telopeptide of type I collagen, CTX) and formation (serum pro-collagen type 1 N-terminal propeptide, P1NP), and site-specific aerial bone mineral density (BMD), trabecular bone score (TBS), in postmenopausal women with osteopenia. The MBPM supplement increased mean daily energy, protein, and calcium intake, by 11, 30, and 107%, respectively. 24-week supplementation with MBPM decreased CTX by 23%, from 0.547 (0.107) to 0.416 (0.087) ng/mL (p < 0.001) and P1NP by 17%, from 60.6 (9.1) to 49.7 (7.2) μg/L (p < 0.001). Compared to CON, MBPM induced a significantly greater reduction in serum CTX (mean (CI95%); −9 (8.6) vs. −23 (8.5)%, p = 0.025 but not P1NP −19 (8.8) vs. −17 (5.2)%, p = 0.802). No significant change in TBS, AP spine or dual femur aerial BMD was observed for CON or MBPM. This study demonstrates the potential benefit of bed-time ingestion of a calcium-fortified, milk-based protein matrix on homeostatic bone remodelling but no resultant treatment effect on site-specific BMD in postmenopausal women with osteopenia.
First Isolation and Characteristics of Bovine Parainfluenza Virus Type 3 from Yaks
Authors: Ren Yunxin , Chen Xi , Tang Cheng , Yue Hua
Published: August 2022

The yaks belong to the genus Bos within the family Bovidae that live in the Tibet Plateau and is an indispensable economic resource for the local herders. Respiratory tract infections are common diseases in yaks caused by various pathogens; however, there have been no reports of bovine parainfluenza virus type 3 (BPIV3) infection. This study was conducted to investigate the pathogens and analyze their characteristics from the four yak lung samples with severe respiratory tract infection symptoms in the yak farm. Results showed that out of four lung samples, three were identified as BPIV3-positive by RT-PCR. A BPIV3 strain (106.5 TCID50/mL) was successfully isolated from the BPIV3-positive lung samples using Madin–Darby bovine kidney cells. The isolate caused systemic infection in the BALB/c mice and induced pathological changes in the lungs. Moreover, three complete BPIV3 genomes were amplified from the clinical samples. Phylogenetic trees based on the complete genomes, hemagglutinin-neuraminidase protein (HN), phosphoprotein (P), and large polymerase subunit protein (L) amino acid sequences showed that the complete BPIV3 genomes belonged to BPIV3 genotype C, and clustered into a large branch with the Chinese strains, although the three yak BPIV3 strains were clustered into a small branch. Compared to known BPIV3 genotype C strains in GenBank, the three genomes of yak BPIV3 showed four identical amino acid mutations in the HN, P and L proteins, suggesting a unique genetic evolution of BPIV3 in yaks. This study first isolated and characterized the BPIV3 from yaks, which contributed to the understanding of the infection and evolution of BPIV3 in yaks in the Tibet Plateau.
Bilosomes as Nanoplatform for Oral Delivery and Modulated In Vivo Antimicrobial Activity of Lycopene
Authors: Binsuwaidan Reem , Sultan Amal A. , Negm Walaa A. , Attallah Nashwah G. M. , Alqahtani Moneerah J. , Hussein Ismail A. , Shaldam Moataz A. , El-Sherbeni Suzy A. , Elekhnawy Engy
Published: August 2022

Owing to the disseminating resistance among pathogenic bacteria, especially Klebsiella pneumoniae, there is a high need for alternate compounds with antibacterial activity. Herein, lycopene was isolated from Lycopersicon esculentum L. Molecular docking approach was employed to explore lycopene binding affinity to selected vital proteins of K. pneumoniae with the binding mechanisms being investigated. This proposed a promising antibacterial activity of lycopene. However, the pharmacological use of lycopene is hampered by its poor solubility and limited oral bioavailability. Accordingly, bilosomes were fabricated for oral lycopene delivery. The computed entrapment efficiency, mean vesicular size, and zeta potential values for the optimized formulation were 93.2 ± 0.6%, 485.8 ± 35.3 nm, and −38.3 ± 4, respectively. In vitro drug release studies revealed controlled lycopene release from constructed bilosomes, with the drug liberation being based on the Higuchi kinetics model. Transmission electron microscopic evaluation of bilosomes revealed spherical nanovesicles free from aggregates. Moreover, the in vitro and in vivo antibacterial activity of lycopene and its constructed formulations against multidrug-resistant K. pneumoniae isolates were explored. The optimized bilosomes exhibited the lowest minimum inhibitory concentrations ranging from 8 to 32 µg/mL. In addition, scanning electron microscopy revealed remarkable deformation and lysis of the bilosomes-treated bacterial cells. Regarding in vivo investigation, a lung infection model in mice was employed. The tested bilosomes reduced the inflammation and congestion in the treated mice’s lung tissues, resulting in normal-sized bronchioles and alveoli with very few congested vessels. In addition, it resulted in a significant reduction in pulmonary fibrosis. In conclusion, this study investigated the potential activity of the naturally isolated lycopene in controlling infections triggered by multidrug-resistant K. pneumoniae isolates. Furthermore, it introduced bilosomes as a promising biocompatible nanocarrier for modulation of oral lycopene delivery and in vivo antimicrobial activity.
Plant Metabolites as SARS-CoV-2 Inhibitors Candidates: In Silico and In Vitro Studies
Authors: Lopes Alberto Jorge Oliveira , Calado Gustavo Pereira , Fróes Yuri Nascimento , Araújo Sandra Alves de , França Lucas Martins , Paes Antonio Marcus de Andrade , Morais Sebastião Vieira de , Rocha Cláudia Quintino da , Vasconcelos Cleydlenne Costa
Published: August 2022

Since it acquired pandemic status, SARS-CoV-2 has been causing all kinds of damage all over the world. More than 6.3 million people have died, and many cases of sequelae are in survivors. Currently, the only products available to most of the world’s population to fight the pandemic are vaccines, which still need improvement since the number of new cases, admissions into intensive care units, and deaths are again reaching worrying rates, which makes it essential to compounds that can be used during infection, reducing the impacts of the disease. Plant metabolites are recognized sources of diverse biological activities and are the safest way to research anti-SARS-CoV-2 compounds. The present study computationally evaluated 55 plant compounds in five SARS-CoV-2 targets such Main Protease (Mpro or 3CL or MainPro), RNA-dependent RNA polymerase (RdRp), Papain-Like Protease (PLpro), NSP15 Endoribonuclease, Spike Protein (Protein S or Spro) and human Angiotensin-converting enzyme 2 (ACE-2) followed by in vitro evaluation of their potential for the inhibition of the interaction of the SARS-CoV-2 Spro with human ACE-2. The in silico results indicated that, in general, amentoflavone, 7-O-galloylquercetin, kaempferitrin, and gallagic acid were the compounds with the strongest electronic interaction parameters with the selected targets. Through the data obtained, we can demonstrate that although the indication of individual interaction of plant metabolites with both Spro and ACE-2, the metabolites evaluated were not able to inhibit the interaction between these two structures in the in vitro test. Despite this, these molecules still must be considered in the research of therapeutic agents for treatment of patients affected by COVID-19 since the activity on other targets and influence on the dynamics of viral infection during the interaction Spro x ACE-2 should be investigated.
Characterization of a Lab-Scale Process to Produce Whole IgG Antivenom Covering Scorpion Stings by Genus Tityus and Centruroides of Colombia
Authors: Estrada-Gomez Sebastian , Núñez Vitelbina , Vargas-Muñoz Leidy Johana , Madrid-Bracamonte Carlos A. , Preciado Lina Maria
Published: August 2022

Scorpion stings are a public health event in Colombia lacking official epidemiological data, and are considered a medical emergency. Despite the two local producers of antivenoms, neither of them is currently manufacturing scorpion antivenoms. We present the characterization of a lab-scale process to produce the first specific scorpion antivenom for Colombia, formulated to cover scorpion stings produced by Tityus pachyurus, Tityus asthenes, Tityus fuhrmanii, Centruroides spp. To do so, rabbits were immunized by subcutaneous injection with each venom using an immunization program of 3 months. After each rabbit reached the required IgG concentration, rabbits were bled, and plasma was separated by decantation under refrigeration. Immunoglobulins were purified from each hyperimmune plasma using a methodology including precipitation with ammonium sulfate, thermocoagulation, and purification through an ultrafiltration process using a ready-to-use and reusable laboratory crossflow tangential cassette with a polyethersulfone membrane. Each hyperimmune plasma was processed by being separated and freeze-dried at the end of the process. Rabbits were able to produce specific IgG antibodies recognizing the respective immunization venom; even an in vitro interspecies cross-recognition was detected. The separation and purification processes allowed us to obtain IgG products without considerable contaminants (except for albumin). The process was characterized, and critical stages were identified.
Immune Modulatory Effects of Molecularly Targeted Therapy and Its Repurposed Usage in Cancer Immunotherapy
Authors: Zhang Tiancheng , Zhang Chenhao , Fu Zile , Gao Qiang
Published: August 2022

The fast evolution of anti-tumor agents embodies a deeper understanding of cancer pathogenesis. To date, chemotherapy, targeted therapy, and immunotherapy are three pillars of the paradigm for cancer treatment. The success of immune checkpoint inhibitors (ICIs) implies that reinstatement of immunity can efficiently control tumor growth, invasion, and metastasis. However, only a fraction of patients benefit from ICI therapy, which turns the spotlight on developing safe therapeutic strategies to overcome the problem of an unsatisfactory response. Molecular-targeted agents were designed to eliminate cancer cells with oncogenic mutations or transcriptional targets. Intriguingly, accumulating shreds of evidence demonstrate the immunostimulatory or immunosuppressive capacity of targeted agents. By virtue of the high attrition rate and cost of new immunotherapy exploration, drug repurposing may be a promising approach to discovering combination strategies to improve response to immunotherapy. Indeed, many clinical trials investigating the safety and efficacy of the combination of targeted agents and immunotherapy have been completed. Here, we review and discuss the effects of targeted anticancer agents on the tumor immune microenvironment and explore their potential repurposed usage in cancer immunotherapy.
Nanostructured Lipid Carrier-Based Codelivery of Raloxifene and Naringin: Formulation, Optimization, In Vitro, Ex Vivo, In Vivo Assessment, and Acute Toxicity Studies
Authors: Alhalmi Abdulsalam , Amin Saima , Khan Zafar , Beg Sarwar , Al kamaly Omkulthom , Saleh Asmaa , Kohli Kanchan
Published: August 2022

This work aimed to develop dual drug-loaded nanostructured lipid carriers of raloxifene and naringin (RLX/NRG NLCs) for breast cancer. RLX/NRG NLCs were prepared using Compritol 888 ATO and oleic acid using a hot homogenization–sonication method and optimized using central composite design (CCD). The optimized RLX/NRG NLCs were characterized and evaluated using multiple technological means. The optimized RLX/NRG NLCs exhibited a particle size of 137.12 nm, polydispersity index (PDI) of 0.266, zeta potential (ZP) of 25.9 mV, and entrapment efficiency (EE) of 91.05% (raloxifene) and 85.07% (naringin), respectively. In vitro release (81 ± 2.2% from RLX/NRG NLCs and 31 ± 1.9% from the RLX/NRG suspension for RLX and 93 ± 1.5% from RLX/NRG NLCs and 38 ± 2.01% from the RLX/NRG suspension for NRG within 24 h). Concurrently, an ex vivo permeation study exhibited nearly 2.3 and 2.1-fold improvement in the permeability profiles of RLX and NRG from RLX/NRG NLCs vis-à-vis the RLX/NRG suspension. The depth of permeation was proved with CLSM images which revealed significant permeation of the drug from the RLX/NRG NLCs formulation, 3.5-fold across the intestine, as compared with the RLX/NRG suspension. An in vitro DPPH antioxidant study displayed a better antioxidant potential of RLX/NRG in comparison to RLX and NRG alone due to the synergistic antioxidant effect of RLX and NRG. An acute toxicity study in Wistar rats showed the safety profile of the prepared nanoformulations and their excipients. Our findings shed new light on how poorly soluble and poorly permeable medicines can be codelivered using NLCs in an oral nanoformulation to improve their medicinal performance.
The Very Long Lifetime of 14C in the Shell Model
Authors: Talmi Igal
Published: August 2022

This is a fitting memory for our late friend and colleague Aldo Covello. For many years, he was our host in the series of Spring Seminars which he organized. In these conferences, the shell model was a central subject which was taken very seriously. This paper is written after 70 years of successful shell model calculations of nuclear energies and also various transitions. The beta decay of 14C has been an enigma. The history and present situation are described. The importance check of any theory to yield the strength of the mirror transition of 14O is pointed out.
Physiological Changes of Arabica Coffee under Different Intensities and Durations of Water Stress in the Brazilian Cerrado
Authors: Silva Patrícia Carvalho da , Junior Walter Quadros Ribeiro , Ramos Maria Lucrecia Gerosa , Rocha Omar Cruz , Veiga Adriano Delly , Silva Nathalia Henriques , Brasileiro Lemerson de Oliveira , Santana Charles Cardoso , Soares Guilherme Filgueiras , Malaquias Juaci Vitória , Vinson Christina Cleo
Published: August 2022

Coffee farmers have faced problems due to drought periods, with irrigation being necessary. In this sense, this study aimed to evaluate the responses to different levels and durations of water deficit in arabica coffee genotypes in the Cerrado region. The experiment consisted of three Coffea arabica genotypes and five water regimes: full irrigation (FI 100 and FI 50—full irrigation with 100% and 50% replacement of evapotranspiration, respectively), water deficit (WD 100 and WD 50—water deficit from June to September, with 100% and 50% replacement of evapotranspiration, respectively) and rainfed (without irrigation). The variables evaluated were gas exchange, relative water content (RWC) and productivity. The results showed that during stress, plants under the FI water regime showed higher gas exchange and RWC, differently from what occurred in the WD and rainfed treatments; however, after irrigation, coffee plants under WDs regained their photosynthetic potential. Rainfed and WD 50 plants had more than 50% reduction in RWC compared to FIs. The Iapar 59 cultivar was the most productive genotype and the E237 the lowest. Most importantly, under rainfed conditions, the plants showed lower physiological and productive potential, indicating the importance of irrigation in Coffea arabica in the Brazilian Cerrado.
The Taxonomically Richest Liverwort Hemiboreal Flora in Eurasia Is in the South Kurils
Authors: Bakalin Vadim A. , Klimova Ksenia G. , Bakalin Daniil A. , Choi Seung Se
Published: August 2022

The long coexistence of various floral elements, landscape diversity, and island isolation led to the formation of the richest Eurasian hemiboreal liverwort flora in the southern Kurils. This land that covers less than 5000 square kilometres and houses 242 species and two varieties of liverworts and hornworts. The flora ‘core’ is represented by hemiboreal East Asian and boreal circumpolar taxa. Other elements that have noticeable input in the flora formation are cool-temperate East Asian hypoarctomontane circumpolar and arctomontane. The distribution of some species is restricted to the thermal pools near active or dormant volcanoes or volcanic ash deposits; such species generally provide specificity to the flora. Despite the territorial proximity, the climate of each considered island is characterized by features that, in the vast majority of cases, distinguish it from the climate of the neighbouring island. The last circumstance may inspire the difference in the liverwort taxonomic composition of each of the islands. The comparison of the taxonomic composition of district floras in the Amphi-Pacific hemiarctic, boreal, and cool-temperate Asia revealed four main focal centres: East Kamchatka Peninsula and Sakhalin Island, the southern Sikhote-Alin and the East Manchurian Mountains, the mountains of the southern part of the Korean Peninsula, and the South Kurils plus northern Hokkaido. The remaining floras involved in the comparison occupy an intermediate position between these four centres.
Durability of CFRP–Steel Double–Lap Joints under Cyclic Freeze–Thaw/Wet–Dry Conditions
Authors: Ren Xiang , Jiang Lingzhi , He Jun , Yang Yi , Sun Yamin , Liu Qunfeng , Chen Shaojie
Published: August 2022

The usage of carbon fiber–reinforced polymer (CFRP) to strengthen cracked steel structures can effectively improve its bear capacity, so it has been extensively used in recent years. The degradation of interfacial bonding is one of the most important factors affecting the durability of CFRP–steel structures under a freeze–thaw(F–T)/wet–dry (W–D) environment. In this study, epoxy resin adhesive (ERA) dog-bone specimens and CFRP–steel double-lap joints (bonded joints) were prepared. F–T/W–D cycles experiment and tensile tests of the ERA specimens and the bonded joints were also performed. Under F–T/W–D cycles, the main properties of the ERA specimens and the bonded joints were examined. Results indicated that fracture failure occurred in all ERA specimens. The hybrid failure modes of fiber peeling on the surface of CFRP plate and the bonded interface peeling between the CFRP plate and ERA layer primarily occurred in the bonded joints. The failure of both of them can be considered to be brittle, which was unaffected by the F–T/W–D cycles. With increased F–T/W–D cycles, the ultimate load and tensile strength of the ERA specimens initially increased and then decreased, whereas the elastic modulus initially increased and then remained unchanged. The ultimate load of the bonded joints decreased gradually. Based on the relationship between the interfacial bond-slip parameters and the number of F–T/W–D cycles, the bond–slip model of the bonded joints was established. The proposed relationship was validated by comparing with the experimental bond-slip relationships and the predicted relationships under the F–T/W–D cycles.
Embossing Lines and Dots Geometry Effect on the Key Tissue Paper Properties with Finite Element Method Analysis
Authors: Vieira Joana Costa , Mendes António de O. , Ribeiro Marcelo Leite , Vieira André Costa , Carta Ana Margarida , Fiadeiro Paulo Torrão , Costa Ana Paula
Published: August 2022

Embossing is a functional and strategic process for creating high-quality multi-sensory tissue-paper products. Embossing modifies the sheet surface by generating hill and/or valley designs, changing the third-dimension z with a compressive die. This research work specifically concerns the impact study of the engraving finishing geometry on the final properties of tissue paper. This work led us to conclude that, even though the sheets individually present a higher hand-feel (HF) value for the straight finishing geometry, the highest softness was obtained in the two-ply prototype for the round finishing geometry. Moreover, this study confirmed that the HF value reduces with the increase of the bulk, being more accentuated for the micropattern. Relevant differences could not be seen in the spreading kinetics of the liquid droplets over time. Thus, the finishing geometry of the 3D plates did not impact the absorption kinetics on these samples. The finite element model allows us to understand the effect of the plate pattern and its finishing geometry on the paper, and the simulation results were in accordance with the experimental results, showing the same trend where patterns with a round finishing geometry marked the tissue-paper sheet more than patterns with a straight finishing did.
Using Cellulose-graft-Poly(L-lactide) Copolymers as Effective Compatibilizers for the Preparation of Cellulose/Poly(L-lactide) Composites with Enhanced Interfacial Compatibility
Authors: Liu Fei , Lu Shan , Cao Weihong , Huang Juncheng , Sun Yi , Xu Yiting , Chen Meiling , Na Haining , Zhu Jin
Published: August 2022

Cellulose-grafte-poly(L-lactide) (C-g-PLLA) copolymers synthesized in a CO2-switchable solvent are proposed for use as effective compatibilizers for the preparation of cellulose–PLLA composites with enhanced interfacial compatibility. The effect of the molar substitution (MSPLLA) of the grafted PLLA side chain in the C-g-PLLA copolymer and the feeding amount of this copolymer on the mechanical and thermal properties and hydrophilicity of the composites was investigated. The composites had a largely increased impact strength with the incorporation of the compatibilizer. With the increasing of MSPLLA and the feeding amount of the copolymer, the resulting composites had an increased impact strength. When 5 wt% C-g-PLLA with MSPLLA of 4.46 was used as a compatibilizer, the obtained composite containing 20 wt% cellulose presented an impact strength equal to that obtained for the neat PLLA. The composites had a slightly decreased melting temperature and thermal decomposition temperature, but increased hydrophilicity due to the incorporation of the compatibilizer. This work suggests an effective method to improve the interfacial compatibility between cellulose and PLLA for the fabrication of fully bio-based composites with high performance.
Improving the Processability and Performance of Micronized Fiber-Reinforced Green Composites through the Use of Biobased Additives
Authors: Valente Bruno F. A. , Silvestre Armando J. D. , Neto Carlos Pascoal , Vilela Carla , Freire Carmen S. R.
Published: August 2022

Green composites made of bioplastics reinforced with natural fibers have gained considerable attention over recent years. However, the use of natural fibers in composites usually compromise some key properties, such as the impact strength and the processability of the final materials. In the present study, two distinct additives, namely an epoxidized linseed oil (ELO) and a sugar-based surfactant, viz. GlucoPure® Sense (GPS), were tested in composite formulations of poly(lactic acid) (PLA) or poly(hydroxybutyrate) (PHB) reinforced with micronized pulp fibers. Both additives showed a plasticizing effect, which led to a decrease in the Young’s and flexural moduli and strengths. At the same time, the elongation and flexural strain at break were considerably improved on some formulations. The melt flow rate was also remarkably improved with the incorporation of the additives. In the PHB-based composites, an increment of 230% was observed upon incorporation of 7.5 wt.% ELO and, in composites based on PLA, an increase of around 155% was achieved with the introduction of 2.5 wt.% GPS. ELO also increased the impact strength to a maximum of 29 kJ m−2, in formulations with PLA. For most composites, a faster degradation rate was observed on the formulations with the additives, reaching, in the case of PHB composites with GPS, a noteworthy weight loss over 75% under burial testing in compost medium at room temperature.
The Investigation of Volatile Organic Compounds (VOCs) Emissions in Environmentally Friendly Modified Asphalt
Authors: Chen Shuoqiu , Wang Jiaqing , Li Qiang , Zhang Wenxuan , Yan Chaojie
Published: August 2022

Asphalt pavements are increasingly used in road engineering; however, the release of volatile organic compounds (VOCs) from asphalt can harm the environment and humans. In this study, different types of modifiers are added to 70# virgin asphalt to prepare environmentally friendly modified asphalt, and its performance is analyzed. Through the self-designed simple asphalt heating-emission collection and detection device, the inhibition effect of different types of modifier combinations on VOCs in the asphalt emmissions was explored. Then, VOCs emission curves of modified asphalt at different temperatures were studied, and finally the basic physical properties of the environmentally friendly modified asphalt were tested. The test results showed that the optimal modifier combination was 5% activated carbon and 3% surfactant, in which the VOCs and the peak value of asphalt heating emissions were only 1385 min·ppm and 86 ppm, respectively, which represented the best VOCs suppression effect of other groups. At the same time, the modified asphalt with optimal additives improved the high-temperature performance of 70# base asphalt and did not affect the storage stability.
Bringing Chinese Christianity to Southeast Asia: Constructing Transnational Chinese Evangelicalism across China and Southeast Asia, 1930s to 1960s
Authors: Sim Daowei (Joshua)
Published: August 2022

This paper takes its cue from studies in Chinese religious transnationalism to offer an interpretation of how a group of Chinese evangelical leaders constructed their visions and versions of transnational Christianity across China and Southeast Asia through the 1930s and 1960s. Two representative organisations are examined. The first concerns the transnational network of Chinese evangelistic bands that the prominent revivalist-evangelist John Sung established across China and Southeast Asia in the 1930s and 1940s. The bands’ sources reveal how they played a key role in imbuing a transnational landscape and communal sense of spiritual revival into the imaginations of the Chinese churches. The second case evaluates the cross-border institutional-building work of the Evangelize China Fellowship, a major transnational Chinese evangelical grouping founded by Sung’s colleague Andrew Gih after World War II. The analysis reveals how the Fellowship utilised a faith-based developmental agenda to promote Christianity among the overseas Chinese communities across Southeast Asia, Taiwan and Hong Kong in the 1950s to 1960s. In all, paying attention to Chinese Christian imaginaries of Southeast Asia enables us to understand how they formed faith adherents across Asia into transnational ethno-religious communities.
The Theological Structure of Evolutionary Theory
Authors: Hunter Cornelius G.
Published: August 2022

Evolutionary theory has greatly influenced science and technology, but in recent decades contemporary scholarship has demonstrated religious influence in evolutionary thought. Religious premises do not merely provide insight; they underwrite evolutionary theory both by mandating strictly naturalistic origins and by providing key arguments for evolution. These arguments are common in the evolution literature, but what are the theological traditions and doctrines underlying these arguments? This paper presents a survey of the historical context of five theological traditions that have been important in the evolution literature. This is a step toward a better understanding of this highly influential theory.
An Improved Apple Object Detection Method Based on Lightweight YOLOv4 in Complex Backgrounds
Authors: Zhang Chenxi , Kang Feng , Wang Yaxiong
Published: August 2022

Convolutional neural networks have recently experienced successful development in the field of computer vision. In precision agriculture, apple picking robots use computer vision methods to detect apples in orchards. However, existing object detection algorithms often face problems such as leaf shading, complex illumination environments, and small, dense recognition targets, resulting in low apple detection rates and inaccurate localization. In view of these problems, we designed an apple detection model based on lightweight YOLOv4—called Improved YOLOv4—from the perspective of industrial application. First, to improve the detection accuracy while reducing the amount of computation, the GhostNet feature extraction network with a Coordinate Attention module is implemented in YOLOv4, and depth-wise separable convolution is introduced to reconstruct the neck and YOLO head structures. Then, a Coordinate Attention module is added to the feature pyramid network (FPN) structure in order to enhance the feature extraction ability for medium and small targets. In the last 15% of epochs in training, the mosaic data augmentation strategy is turned off in order to further improve the detection performance. Finally, a long-range target screening strategy is proposed for standardized dense planting apple orchards with dwarf rootstock, removing apples in non-target rows and improving detection performance and recognition speed. On the constructed apple data set, compared with YOLOv4, the mAP of Improved YOLOv4 was increased by 3.45% (to 95.72%). The weight size of Improved YOLOv4 is only 37.9 MB, 15.53% of that of YOLOv4, and the detection speed is improved by 5.7 FPS. Two detection methods of similar size—YOLOX-s and EfficientNetB0-YOLOv3—were compared with Improved YOLOv4. Improved YOLOv4 outperformed these two algorithms by 1.82% and 2.33% mAP, respectively, on the total test set and performed optimally under all illumination conditions. The presented results indicate that Improved YOLOv4 has excellent detection accuracy and good robustness, and the proposed long-range target screening strategy has an important reference value for solving the problem of accurate and rapid identification of various fruits in standard orchards.