bluuebunny/crossref_metadata_2025 huggingface dataset:
Involvement of the Protein Ras Homolog Enriched in the Striatum, Rhes, in Dopaminergic Neurons’ Degeneration: Link to Parkinson’s Disease
Authors: Serra Marcello , Pinna Annalisa , Costa Giulia , Usiello Alessandro , Pasqualetti Massimo , Avallone Luigi , Morelli Micaela , Napolitano Francesco
Published: May 2021

Rhes is one of the most interesting genes regulated by thyroid hormones that, through the inhibition of the striatal cAMP/PKA pathway, acts as a modulator of dopamine neurotransmission. Rhes mRNA is expressed at high levels in the dorsal striatum, with a medial-to-lateral expression gradient reflecting that of both dopamine D2 and adenosine A2A receptors. Rhes transcript is also present in the hippocampus, cerebral cortex, olfactory tubercle and bulb, substantia nigra pars compacta (SNc) and ventral tegmental area of the rodent brain. In line with Rhes-dependent regulation of dopaminergic transmission, data showed that lack of Rhes enhanced cocaine- and amphetamine-induced motor stimulation in mice. Previous studies showed that pharmacological depletion of dopamine significantly reduces Rhes mRNA levels in rodents, non-human primates and Parkinson’s disease (PD) patients, suggesting a link between dopaminergic innervation and physiological Rhes mRNA expression. Rhes protein binds to and activates striatal mTORC1, and modulates L-DOPA-induced dyskinesia in PD rodent models. Finally, Rhes is involved in the survival of mouse midbrain dopaminergic neurons of SNc, thus pointing towards a Rhes-dependent modulation of autophagy and mitophagy processes, and encouraging further investigations about mechanisms underlying dysfunctions of the nigrostriatal system.
Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells
Authors: Son Yeonghoon , Shin Na-Rae , Kim Sung-Ho , Park Su-Cheol , Lee Hae-June
Published: May 2021

Despite liver cancer being the second-leading cause of cancer-related death worldwide, few systemic drugs have been approved. Sorafenib, the first FDA-approved systemic drug for unresectable hepatocellular carcinoma (HCC), is limited by resistance. However, the precise mechanisms underlying this phenomenon are unknown. Since fibrinogen-like 1 (FGL1) is involved in HCC progression and upregulated after anticancer therapy, we investigated its role in regulating sorafenib resistance in HCC. FGL1 expression was assessed in six HCC cell lines (HepG2, Huh7, Hep3B, SNU387, SNU449, and SNU475) using western blotting. Correlations between FGL1 expression and sorafenib resistance were examined by cell viability, colony formation, and flow cytometry assays. FGL1 was knocked-down to confirm its effects on sorafenib resistance. FGL1 expression was higher in HepG2, Huh7, and Hep3B cells than in SNU387, SNU449, and SNU475 cells; high FGL1-expressing HCC cells showed a lower IC50 and higher sensitivity to sorafenib. In Huh7 and Hep3B cells, FGL1 knockdown significantly increased colony formation by 61% (p = 0.0013) and 99% (p = 0.0002), respectively, compared to that in controls and abolished sorafenib-induced suppression of colony formation, possibly by modulating ERK and autophagy signals. Our findings demonstrate that sorafenib resistance mediated by FGL1 in HCC cells, suggesting FGL1 as a potential sorafenib-resistance biomarker and target for HCC therapy.
Ectopic Expression of a Heterologous Glutaredoxin Enhances Drought Tolerance and Grain Yield in Field Grown Maize
Authors: Tamang Tej Man , Sprague Stuart A. , Kakeshpour Tayebeh , Liu Sanzhen , White Frank F. , Park Sunghun
Published: May 2021

Drought stress is a major constraint in global maize production, causing almost 30–90% of the yield loss depending upon growth stage and the degree and duration of the stress. Here, we report that ectopic expression of Arabidopsis glutaredoxin S17 (AtGRXS17) in field grown maize conferred tolerance to drought stress during the reproductive stage, which is the most drought sensitive stage for seed set and, consequently, grain yield. AtGRXS17-expressing maize lines displayed higher seed set in the field, resulting in 2-fold and 1.5-fold increase in yield in comparison to the non-transgenic plants when challenged with drought stress at the tasseling and silking/pollination stages, respectively. AtGRXS17-expressing lines showed higher relative water content, higher chlorophyll content, and less hydrogen peroxide accumulation than wild-type (WT) control plants under drought conditions. AtGRXS17-expressing lines also exhibited at least 2-fold more pollen germination than WT plants under drought stress. Compared to the transgenic maize, WT controls accumulated higher amount of proline, indicating that WT plants were more stressed over the same period. The results present a robust and simple strategy for meeting rising yield demands in maize under water limiting conditions.
Noscapine Acts as a Protease Inhibitor of In Vitro Elastase-Induced Collagen Deposition in Equine Endometrium
Authors: Amaral Ana , Fernandes Carina , Szóstek-Mioduchowska Anna , Rebordão Maria Rosa , Skarzynski Dariusz Jan , Ferreira-Dias Graça
Published: May 2021

Endometrosis is a reproductive pathology that is responsible for mare infertility. Our recent studies have focused on the involvement of neutrophil extracellular traps enzymes, such as elastase (ELA), in the development of equine endometrosis. Noscapine (NOSC) is an alkaloid derived from poppy opium with anticough, antistroke, anticancer, and antifibrotic properties. The present work investigates the putative inhibitory in vitro effect of NOSC on collagen type I alpha 2 chain (COL1A2) mRNA and COL1 protein relative abundance induced by ELA in endometrial explants of mares in the follicular or mid-luteal phases at 24 or 48 h of treatment. The COL1A2 mRNA was evaluated by qPCR and COL1 protein relative abundance by Western blot. In equine endometrial explants, ELA increased COL 1 expression, while NOSC inhibited it at both estrous cycle phases and treatment times. These findings contribute to the future development of new endometrosis treatment approaches. Noscapine could be a drug capable of preventing collagen synthesis in mare’s endometrium and facilitate the therapeutic approach.
FLTX2: A Novel Tamoxifen Derivative Endowed with Antiestrogenic, Fluorescent, and Photosensitizer Properties
Authors: Díaz Mario , Lobo Fernando , Hernández Dácil , Amesty Ángel , Valdés-Baizabal Catalina , Canerina-Amaro Ana , Mesa-Herrera Fátima , Soler Kevin , Boto Alicia , Marín Raquel , Estévez-Braun Ana , Lahoz Fernando
Published: May 2021

Tamoxifen is the most widely used selective modulator of estrogen receptors (SERM) and the first strategy as coadjuvant therapy for the treatment of estrogen-receptor (ER) positive breast cancer worldwide. In spite of such success, tamoxifen is not devoid of undesirable effects, the most life-threatening reported so far affecting uterine tissues. Indeed, tamoxifen treatment is discouraged in women under risk of uterine cancers. Recent molecular design efforts have endeavoured the development of tamoxifen derivatives with antiestrogen properties but lacking agonistic uterine tropism. One of this is FLTX2, formed by the covalent binding of tamoxifen as ER binding core, 7-nitrobenzofurazan (NBD) as the florescent dye, and Rose Bengal (RB) as source for reactive oxygen species. Our analyses demonstrate (1) FLTX2 is endowed with similar antiestrogen potency as tamoxifen and its predecessor FLTX1, (2) shows a strong absorption in the blue spectral range, associated to the NBD moiety, which efficiently transfers the excitation energy to RB through intramolecular FRET mechanism, (3) generates superoxide anions in a concentration- and irradiation time-dependent process, and (4) Induces concentration- and time-dependent MCF7 apoptotic cell death. These properties make FLTX2 a very promising candidate to lead a novel generation of SERMs with the endogenous capacity to promote breast tumour cell death in situ by photosensitization.
Long Non-Coding RNA MEG3 in Cellular Stemness
Authors: Hsieh Pei-Fang , Yu Cheng-Chia , Chu Pei-Ming , Hsieh Pei-Ling
Published: May 2021

Long non-coding RNAs (lncRNAs) regulate a diverse array of cellular processes at the transcriptional, post-transcriptional, translational, and post-translational levels. Accumulating evidence suggests that lncRNA MEG3 exerts a large repertoire of regulatory functions in cellular stemness. This review focuses on the molecular mechanisms by which lncRNA MEG3 functions as a signal, scaffold, guide, and decoy for multi-lineage differentiation and even cancer progression. The role of MEG3 in various types of stem cells and cancer stem cells is discussed. Here, we provide an overview of the functional versatility of lncRNA MEG3 in modulating pluripotency, differentiation, and cancer stemness.
Long-Term Exposure to Nanosized TiO2 Triggers Stress Responses and Cell Death Pathways in Pulmonary Epithelial Cells
Authors: Alswady-Hoff Mayes , Erdem Johanna Samulin , Phuyal Santosh , Knittelfelder Oskar , Sharma Animesh , Fonseca Davi de Miranda , Skare Øivind , Slupphaug Geir , Zienolddiny Shanbeh
Published: May 2021

There is little in vitro data available on long-term effects of TiO2 exposure. Such data are important for improving the understanding of underlying mechanisms of adverse health effects of TiO2. Here, we exposed pulmonary epithelial cells to two doses (0.96 and 1.92 µg/cm2) of TiO2 for 13 weeks and effects on cell cycle and cell death mechanisms, i.e., apoptosis and autophagy were determined after 4, 8 and 13 weeks of exposure. Changes in telomere length, cellular protein levels and lipid classes were also analyzed at 13 weeks of exposure. We observed that the TiO2 exposure increased the fraction of cells in G1-phase and reduced the fraction of cells in G2-phase, which was accompanied by an increase in the fraction of late apoptotic/necrotic cells. This corresponded with an induced expression of key apoptotic proteins i.e., BAD and BAX, and an accumulation of several lipid classes involved in cellular stress and apoptosis. These findings were further supported by quantitative proteome profiling data showing an increase in proteins involved in cell stress and genomic maintenance pathways following TiO2 exposure. Altogether, we suggest that cell stress response and cell death pathways may be important molecular events in long-term health effects of TiO2.
Beneficial Effects of Metformin on the Central Nervous System, with a Focus on Epilepsy and Lafora Disease
Authors: Sanz Pascual , Serratosa José Maria , Sánchez Marina P.
Published: May 2021

Metformin is a drug in the family of biguanide compounds that is widely used in the treatment of type 2 diabetes (T2D). Interestingly, the therapeutic potential of metformin expands its prescribed use as an anti-diabetic drug. In this sense, it has been described that metformin administration has beneficial effects on different neurological conditions. In this work, we review the beneficial effects of this drug as a neuroprotective agent in different neurological diseases, with a special focus on epileptic disorders and Lafora disease, a particular type of progressive myoclonus epilepsy. In addition, we review the different proposed mechanisms of action of metformin to understand its function at the neurological level.
Antimicrobial and Anti-Biofilm Peptide Octominin for Controlling Multidrug-Resistant Acinetobacter baumannii
Authors: Jayathilaka E. H. T. Thulshan , Rajapaksha Dinusha C. , Nikapitiya Chamilani , De Zoysa Mahanama , Whang Ilson
Published: May 2021

Acinetobacter baumannii is a serious nosocomial pathogen with multiple drug resistance (MDR), the control of which has become challenging due to the currently used antibiotics. Our main objective in this study is to determine the antibacterial and antibiofilm activities of the antimicrobial peptide, Octominin, against MDR A. baumannii and derive its possible modes of actions. Octominin showed significant bactericidal effects at a low minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of 5 and 10 µg/mL, respectively. Time-kill kinetic analysis and bacterial viability tests revealed that Octominin showed a concentration-dependent antibacterial activity. Field-emission scanning electron microscopy (FE-SEM) analysis revealed that Octominin treatment altered the morphology and membrane structure of A. baumannii. Propidium iodide (PI) and reactive oxygen species (ROS) generation assays showed that Octominin increased the membrane permeability and ROS generation in A. baumannii, thereby causing bacterial cell death. Further, a lipopolysaccharides (LPS) binding assay showed an Octominin concentration-dependent LPS neutralization ability. Biofilm formation inhibition and eradication assays further revealed that Octominin inhibited biofilm formation and showed a high biofilm eradication activity against A. baumannii. Furthermore, up to a concentration of 100 µg/mL, Octominin caused no hemolysis and cell viability changes in mammalian cells. An in vivo study in zebrafish showed that the Octominin-treated group had a significantly higher relative percentage survival (54.1%) than the untreated group (16.6%). Additionally, a reduced bacterial load and fewer alterations in histological analysis confirmed the successful control of A. baumannii by Octominin in vivo. Collectively, these data suggest that Octominin exhibits significant antibacterial and antibiofilm activities against the multidrug-resistant A. baumannii, and this AMP can be developed further as a potent AMP for the control of antibiotic resistance.
The CD200 Regulates Inflammation in Mice Independently of TNF-α Production
Authors: Tonecka Katarzyna , Braniewska Agata , Pilch Zofia , Sas Zuzanna , Skorzynski Marcin , Manuali Elisabetta , Rygiel Tomasz P.
Published: May 2021

Inflammatory bowel disease is characterized by the infiltration of immune cells and chronic inflammation. The immune inhibitory receptor, CD200R, is involved in the downregulation of the activation of immune cells to prevent excessive inflammation. We aimed to define the role of CD200R ligand-CD200 in the experimental model of intestinal inflammation in conventionally-reared mice. Mice were given a dextran sodium sulfate solution in drinking water. Bodyweight loss was monitored daily and the disease activity index was calculated, and a histological evaluation of the colon was performed. TNF-α production was measured in the culture of small fragments of the distal colon or bone marrow-derived macrophages (BMDMs) cocultured with CD200+ cells. We found that Cd200−/− mice displayed diminished severity of colitis when compared to WT mice. Inflammation significantly diminished CD200 expression in WT mice, particularly on vascular endothelial cells and immune cells. The co-culture of BMDMs with CD200+ cells inhibited TNF-α secretion. In vivo, acute colitis induced by DSS significantly increased TNF-α secretion in colon tissue in comparison to untreated controls. However, Cd200−/− mice secreted a similar level of TNF-α to WT mice in vivo. CD200 regulates the severity of DSS-induced colitis in conventionally-reared mice. The presence of CD200+ cells decreases TNF-α production by macrophages in vitro. However, during DDS-induced intestinal inflammation secretion of TNF-α is independent of CD200 expression.
BPA, BPAF and TMBPF Alter Adipogenesis and Fat Accumulation in Human Mesenchymal Stem Cells, with Implications for Obesity
Authors: Cohen Isabel C. , Cohenour Emry R. , Harnett Kristen G. , Schuh Sonya M.
Published: May 2021

Bisphenol A (BPA) is an endocrine-disrupting chemical used in the production of plastics, and is linked to developmental, reproductive, and metabolic disorders including obesity. Manufacturers have begun using ‘BPA-free’ alternatives instead of BPA in many consumer products. However, these alternatives have had much less testing and oversight, yet they are already being mass-produced and used across industries from plastics to food-contact coatings. Here, we used human female adipose-derived stem cells (hASCs), a type of adult mesenchymal stem cell, to compare the effects of BPA and BPA alternatives on adipogenesis or fat cell development in vitro. We focused on two commonly used BPA replacements, bisphenol AF (BPAF) and tetramethyl bisphenol F (TMBPF; monomer of the new valPure V70 food-contact coating). Human ASCs were differentiated into adipocytes using chemically defined media in the presence of control differentiation media with and without 17β-estradiol (E2; 10 μM), or with increasing doses of BPA (0, 0.1 and 1 μM), BPAF (0, 0.1, 1 and 10 nM), or TMBPF (0, 0.01 and 0.1 μM). After differentiation, the cells were stained and imaged to visualize and quantify the accumulation of lipid vacuoles and number of developing fat cells. Treated cells were also examined for cell viability and apoptosis (programmed cell death) using the respective cellular assays. Similar to E2, BPA at 0.1 μM and BPAF at 0.1 nM, significantly increased adipogenesis and lipid production by 20% compared to control differentiated cells (based on total lipid vacuole number to cell number ratios), whereas higher levels of BPA and BPAF significantly decreased adipogenesis (p < 0.005). All tested doses of TMBPF significantly reduced adipogenesis and lipid production by 30–40%, likely at least partially through toxic effects on stem cells, as viable cell numbers decreased and apoptosis levels increased throughout differentiation. These findings indicate that low, environmentally-relevant doses of BPA, BPAF, and TMBPF have significant effects on fat cell development and lipid accumulation, with TMBPF having non-estrogenic, anti-adipogenic effects. These and other recent results may provide a potential cellular mechanism between exposure to bisphenols and human obesity, and underscore the likely impact of these chemicals on fat development in vivo.
Improving Whitefly Management
Authors: Simmons Alvin M. , Riley David G.
Published: May 2021

Whiteflies (Hemiptera: Aleyrodidae), especially the sweetpotato or cotton whitefly, Bemisia tabaci (Gennadius), are among the most destructive and difficult to manage polyphagous insect pests around the globe [...]
Pretreatment Neutrophil-to-Lymphocyte Ratio Combined with Platelet-to-Lymphocyte Ratio as a Predictor of Survival Outcomes after Definitive Concurrent Chemoradiotherapy for Cervical Cancer
Authors: Lee Jeong Won , Seol Ki Ho
Published: May 2021

The aim of the study was to evaluate pretreatment neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) as prognostic factors for predicting clinical outcomes after definitive concurrent chemoradiotherapy (CCRT) for cervical cancer. The cases were divided into two groups based on the values of NLR and PLR: High NLR-PLR (high value in both NLR and PLR) and Low NLR-PLR (low value in either NLR or PLR). The relationships between survival outcomes and the pretreatment NLR-PLR were investigated. Of the 148 patients enrolled in the study, 30 patients died during the median follow-up of 75 months. Based on receiver operating curves, NLR and PLR cut-off values for survival analysis were 2.34 and 148.89. The 10-year overall survival and disease-free survival rates for high NLR-PLR vs. low NLR-PLR were 63.6% vs. 86.2% (p = 0.001) and 63.3% vs. 77.5% (p = 0.026), respectively. Based on a multivariate analysis, independent predictors of overall survival were high NLR-PLR (hazard ratio [HR], 2.435; 95% confidence interval [CI], 1.106–5.361; p = 0.027) and stage (HR 2.659; 95% CI, 1.146–6.613; p = 0.024). Increases in both NLR and PLR are associated with poor survival. Elevation in both NLR and PLR before initiation of CCRT may be a useful biomarker for predicting clinical outcomes.
Feasibility and Long-Term Efficacy of PEComa Treatment—20 Years of Experience
Authors: Sobiborowicz Aleksandra , Świtaj Tomasz , Teterycz Paweł , Spałek Mateusz J. , Szumera-Ciećkiewicz Anna , Wągrodzki Michał , Zdzienicki Marcin , Czarnecka Anna M. , Rutkowski Piotr
Published: May 2021

Perivascular epithelioid cell tumors (PEComas) represent a family of rare mesenchymal neoplasms, some of which are malignant. There are no specific management guidelines for PEComas, and factors correlating with the disease course are not well defined. This analysis aimed to describe the outcomes of PEComa patients treated radically, including those treated exclusively in the national reference sarcoma center. The secondary aim of the study was to analyze factors associated with PEComa treatment efficacy. We performed an analysis of 27 patients subsequently treated radically for PEComa between 1999 and 2019 who were in follow-up in the national sarcoma reference center. The proportional-hazards model was used to compare the risk of death. The median age at diagnosis was 45 (21–67) years, and 67% of patients were female. The median follow-up period was 68 months (95% CI: 39–101). At the time of analysis, eleven patients (40.7%) experienced progression of the disease and four (14.8%) died. Surgery in the reference sarcoma center was associated with a longer disease control (log-rank p < 0.001). The 5-year-OS rate was 88% (95% CI: 74–100) for the whole analyzed group. We concluded that PEComa treatment should be managed in reference sarcoma centers by a multidisciplinary tumor board with an experienced surgical team. Microscopically radical resection is associated with a longer disease-free survival. Patients requiring long-term follow-ups as late recurrence may be expected.
Recent Advances in Gestational Diabetes Mellitus
Authors: Benhalima Katrien
Published: May 2021

The incidence of gestational diabetes mellitus (GDM) and overt diabetes in pregnancy is rising globally [...]
Faecal Calprotectin in Assessment of Mucosal Healing in Adults with Inflammatory Bowel Disease: A Meta-Analysis
Authors: Bromke Mariusz A. , Neubauer Katarzyna , Kempiński Radosław , Krzystek-Korpacka Małgorzata
Published: May 2021

Achieving mucosal healing in patients with inflammatory bowel disease is related to a higher incidence of sustained clinical remission and it translates to lower rates of hospitalisation and surgery. The assessment methods of disease activity and response to therapy are limited and mainly rely on colonoscopy. This meta-analysis reviews the effectiveness of using faecal calprotectin as a marker for mucosal healing in inflammatory bowel disease. Two meta-analyses were conducted in parallel. The analysis on the use of faecal calprotectin in monitoring mucosal healing in colonic Crohn’s disease is based on 16 publications (17 studies). The data set for diagnostic values of faecal calprotectin in ulcerative colitis is composed of 35 original publications (total 49 studies). The DOR for the use of faecal calprotectin in Crohn’s disease is estimated to be 11.20 and the area under the sROCis 0.829. In cases of ulcerative colitis, the DOR is 14.48, while the AUC sROC is 0.858. Heterogeneity of the studies was moderatetosubstantial. Collected data show overall good sensitivity and specificity of the faecal calprotectin test, as well as a good DOR. Thus, monitoring of mucosal healing with a non-invasive faecal calprotectin test may represent an attractive option for physicians and patients with inflammatory bowel disease.
Feasibility and Safety of the Left Distal Radial Approach in Percutaneous Coronary Intervention for Bifurcation Lesions
Authors: Lee Oh-Hyun , Roh Ji Woong , Im Eui , Cho Deok-Kyu , Jeong Myung Ho , Choi Donghoon , Kim Yongcheol
Published: May 2021

Recently, the left distal radial approach (DRA) for percutaneous coronary intervention (PCI) has been shown to be a feasible option, but there are limited data regarding the feasibility of performing bifurcation PCI via the left DRA. Therefore, this study aimed to describe our experience with the contemporary left DRA to perform PCI of bifurcation lesions. Between December 2017 and December 2019, we identified 106 patients treated with bifurcation PCI via the left DRA. We evaluated the success rate of PCI, access-site complications including major bleeding requiring surgery or transfusion, hematoma, distal and forearm radial artery occlusion, and 30-day mortality. Eleven patients (10.4%) treated with left main bifurcation and true bifurcations accounted for 39.6% of cases, with the left anterior descending artery/diagonal branch being the most frequent bifurcation site (57.5%, 61/106). PCI was performed using a 6-French guiding catheter in 101 (95.3%) cases. Successful PCI for bifurcation lesions via the left DRA was achieved in all 106 patients without access-site cross-over. There was no major bleeding, distal and forearm radial artery occlusion, forearm hematoma, or mortality at 30 days. The left DRA is a safe and feasible alternative access site for bifurcation PCI in selected patients.
Fine Spatial Scale, Frequent Morphological Monitoring of Urbanised Beaches to Improve Coastal Management
Authors: Hinestroza-Mena Kelis M. , Toro Vladimir G. , Londoño-Colorado Gloria S. , Chávez Valeria , García-Blanco Julieth K. , Silva Rodolfo
Published: May 2021

Between 1959 and 2010, a coastline retreat of 2.4 m/year and erosion of up to 174 m were recorded around Turbo, in northern Colombia. This degraded coastal system is the result of a poorly planned coastal defence scheme, combined with a lack of formal technical methodologies for diagnosis and monitoring. The coastal system cannot provide the protection services required by the local community. From 2017 to 2019, the group monitored urban beach profiles in a small area close to the town of Turbo, in the wet and the dry seasons, as part of a coastline analysis to identify morphodynamic trends in the area. The results show a net shoreline accretion of up to 30 m and positive sedimentary accumulation of up to 45.8 m3/m. To the north of the study area, sediment accumulation is evident at the Turbo River Delta. The 34 coastal protection structures in the study area have a high k index (~0.7), suggesting that they are the main drivers controlling the trend of sediment accumulation. The correlation of geomorphological, oceanographic, and anthropic variables related to the presence of coastal structures, is necessary in order to set up efficient coastal protection schemes.
The Pacific Tree-Parasitic Fungus Cyclocybe parasitica Exhibits Monokaryotic Fruiting, Showing Phenotypes Known from Bracket Fungi and from Cyclocybe aegerita
Authors: Elders Hannah , Hennicke Florian
Published: May 2021

Cyclocybe parasitica is a wood-destroying parasitic edible mushroom growing on diverse broad-leafed trees in New Zealand and other Pacific areas. Recent molecular systematics of European Cyclocybe aegerita, a newly delimited Asian phylum and of related species, corroborated the distinction of the chiefly saprobic cultivated edible mushroom C. aegerita from C. parasitica. Here, we show that C. parasitica exhibits a morpho-physiological trait characteristic to its European cousin, i.e., monokaryotic fruiting sensu stricto (basidiome formation without mating). Monokaryotic fruiting structures formed by C. parasitica ICMP 11668-derived monokaryons were categorized into four phenotypes. One of them displays ulcer-like structures previously reported from bracket fungi. Histology of dikaryotic and monokaryotic C. parasitica fruiting structures revealed anatomical commonalities and differences between them, and towards monokaryotic fruiting structures of C. aegerita. Mating experiments with C. parasitica strains representative of each fruiting phenotype identified compatible sibling monokaryons. Given reports on hypothetically monokaryotic basidiome field populations of ‘C. aegerita sensu lato’, it seems worthwhile to prospectively investigate whether monokaryotic fruiting s.str. occurs in nature. Sampling from such populations including karyotyping, comparative -omics, and competition assays may help to answer this question and provide evidence whether this trait may confer competitive advantages to a species capable of it.
Application of Novel Non-Thermal Physical Technologies to Degrade Mycotoxins
Authors: Yousefi Mohammad , Mohammadi Masoud Aman , Khajavi Maryam Zabihzadeh , Ehsani Ali , Scholtz Vladimír
Published: May 2021

Mycotoxins cause adverse effects on human health. Therefore, it is of the utmost importance to confront them, particularly in agriculture and food systems. Non-thermal plasma, electron beam radiation, and pulsed light are possible novel non-thermal technologies offering promising results in degrading mycotoxins with potential for practical applications. In this paper, the available publications are reviewed—some of them report efficiency of more than 90%, sometimes almost 100%. The mechanisms of action, advantages, efficacy, limitations, and undesirable effects are reviewed and discussed. The first foretastes of plasma and electron beam application in the industry are in the developing stages, while pulsed light has not been employed in large-scale application yet.
Macrofungi as a Nutraceutical Source: Promising Bioactive Compounds and Market Value
Authors: Niego Allen Grace , Rapior Sylvie , Thongklang Naritsada , Raspé Olivier , Jaidee Wuttichai , Lumyong Saisamorn , Hyde Kevin D.
Published: May 2021

Macrofungi production and economic value have been increasing globally. The demand for macrofungi has expanded rapidly owing to their popularity among consumers, pleasant taste, and unique flavors. The presence of high quality proteins, polysaccharides, unsaturated fatty acids, minerals, triterpene sterols, and secondary metabolites makes macrofungi an important commodity. Macrofungi are well known for their ability to protect from or cure various health problems, such as immunodeficiency, cancer, inflammation, hypertension, hyperlipidemia, hypercholesterolemia, and obesity. Many studies have demonstrated their medicinal properties, supported by both in vivo and in vitro experimental studies, as well as clinical trials. Numerous bioactive compounds isolated from mushrooms, such as polysaccharides, proteins, fats, phenolic compounds, and vitamins, possess strong bioactivities. Consequently, they can be considered as an important source of nutraceuticals. Numerous edible mushrooms have been studied for their bioactivities, but only a few species have made it to the market. Many species remain to be explored. The converging trends and popularity of eastern herbal medicines, natural/organic food product preference, gut-healthy products, and positive outlook towards sports nutrition are supporting the growth in the medicinal mushroom market. The consumption of medicinal mushrooms as functional food or dietary supplement is expected to markedly increase in the future. The global medicinal mushroom market size is projected to increase by USD 13.88 billion from 2018 to 2022. The global market values of promising bioactive compounds, such as lentinan and lovastatin, are also expected to rise. With such a market growth, mushroom nutraceuticals hold to be very promising in the years to come.
Spontaneous Cities: Lessons to Improve Planning for Housing
Authors: Salingaros Nikos Angelos
Published: May 2021

The world can learn two key lessons from spontaneous settlements: (i) design so as to adapt to human biology; and (ii) design to save energy. Timeless processes of urban growth and sustainability have forced societies to conserve energy. Yet, nowadays, a profession focused on design ideology and short-term profit discredits many economical and effective long-term design methods. Decision-makers, politicians, and urbanists talk of energy conservation while continuing to use failed notions of industrial urbanity in place of documented solutions that work. Most damaging is the myopic academic elite’s fixation on an unsustainable industrial-modernist visual vocabulary of minimalist forms. By promoting typologies based on images dating from the 1920s, instead of using scientific analysis, the industry serves extractive global imperialism rather than satisfying the world’s population needs. We should instead learn from how self-builders adapt form, geometry, materials, surfaces, and ornament to maximize the user’s emotional experience in an otherwise extremely challenging environment.
Spatial-Temporal Changes and Driving Force Analysis of Green Space in Coastal Cities of Southeast China over the Past 20 Years
Authors: Weng Huayan , Gao Yongchao , Su Xinyi , Yang Xiaodong , Cheng Fangyan , Ma Renfeng , Liu Yanju , Zhang Wen , Zheng Liwen
Published: May 2021

The purpose of this study is to reveal the spatial-temporal change and driving factors of green space in coastal cities of southeast China over the past 20 years. A supervised classification method combining support vector machines (SVMs) and visual interpretation was used to extract the green space from Landsat TM/OLI imageries from 2000–2020. The landscape pattern index was used to calculate geospatial information of green space and analyze their spatial-temporal changes. The hierarchical partitioning analysis was then used to determine the influences of anthropogenic and geographic environmental factors on the spatial-temporal changes in green space. The results indicated that the total area of green space remained constant over the past 20 years in coastal cities of southeast China (1% reduction). The spatial change of green space mainly occurred in the area near the ocean and the southern region. 41.37% of forest land was transferred from cultivated land, while 44.56%, 41.83%, 43.20%, 46.31%, 41.98% and 40.20% of shrub land, sparse woodland, other woodland, high-coverage grassland, moderate-coverage grassland and low-coverage grassland were transferred from forest land. The number of patches, patch density, edge density, landscape shape index and Shannon’s diversity index increased from 2000–2015, and then decreased to the minimum in 2020, while largest patch index continued to decline from 2000–2020. The contribution of anthropogenic factors (0.53–0.61) on the spatial-temporal changes of green space continually increased over the past 20 years, which was also higher than geographical environment factors (0.39–0.41). Our study provides a new perspective to distinguish the impact of anthropogenic activities and geographical environmental factors on the change of green space area, thereby providing a theoretical support for the construction and ecological management of green space.
Pollination in Agroecosystems: A Review of the Conceptual Framework with a View to Sound Monitoring
Authors: Giovanetti Manuela , Albertazzi Sergio , Flaminio Simone , Ranalli Rosa , Bortolotti Laura , Quaranta Marino
Published: May 2021

The pollination ecology in agroecosystems tackles a landscape in which plants and pollinators need to adjust, or be adjusted, to human intervention. A valid, widely applied approach is to regard pollination as a link between specific plants and their pollinators. However, recent evidence has added landscape features for a wider ecological perspective. Are we going in the right direction? Are existing methods providing pollinator monitoring tools suitable for understanding agroecosystems? In Italy, we needed to address these questions to respond to government pressure to implement pollinator monitoring in agroecosystems. We therefore surveyed the literature, grouped methods and findings, and evaluated approaches. We selected studies that may contain directions and tools directly linked to pollinators and agroecosystems. Our analysis revealed four main paths that must come together at some point: (i) the research question perspective, (ii) the advances of landscape analysis, (iii) the role of vegetation, and (iv) the gaps in our knowledge of pollinators taxonomy and behavior. An important conclusion is that the pollinator scale is alarmingly disregarded. Debate continues about what features to include in pollinator monitoring and the appropriate level of detail: we suggest that the pollinator scale should be the main driver.
Spatial Chaos as a Result of War Damage and Post-War Transformations. Example of the Small Town of Węgorzewo
Authors: Musiaka Łukasz , Sudra Paweł , Spórna Tomasz
Published: May 2021

World War II’s military activities and the post-war devastation period destroyed many European cities and towns. One of the areas that was struck the most was former East Prussia, currently located in Poland and the Kaliningrad Region (the Russian Federation). In addition to the destruction of cities, which are strategically and economically important, small towns have also suffered. An example of such a town is Węgorzewo, where the scale of destruction of the pre-war urban tissue exceeded 80%, and the old town’s built-up area practically ceased to exist. This town magnifies most of the processes and spatial problems characteristic of Central and Eastern Europe’s towns of the “metamorphic” type. Post-war zoning during the Polish People’s Republic period, in the spirit of constructing a socialist town and bypassing the original spatial arrangement, brought about irreversible changes in the urban tissue. This was reflected in the break with the town’s original layout and the creation of modernist buildings. The changes were solidified or even deepened during the economic and political transition of the 1990s in Poland. Today, decades after the end of World War II, despite taking corrective measures, the town is still facing the problem of spatial chaos. Its morphological and physiognomic manifestations in the lack of a central public space, the loss of its historic character, the disharmonization of the urban landscape, and the dispersed development are the main subjects of this article’s analysis. This study uses a diverse methodological apparatus consisting of an analysis of the town’s morphological transformations, an analysis of the physiognomy of the urban landscape and architecture, in situ studies, and an analysis of municipal documents and expert interviews. In the discussion, the study results are embedded in the context of the cases of other European cities and towns. The conclusions indicate the risks to the formation of spatial order in Węgorzewo and possible paths of action.
Antioxidant Status in the Vitreous of Eyes with Rhegmatogenous Retinal Detachment with and without Proliferative Vitreoretinopathy, Macular Hole and Epiretinal Membrane
Authors: Pietras-Baczewska Agata , Nowomiejska Katarzyna , Brzozowska Agnieszka , Toro Mario Damiano , Załuska Wojciech , Sztanke Małgorzata , Sztanke Krzysztof , Rejdak Robert
Published: May 2021

(1) Background: The aim of the study was to test the hypothesis that the antioxidant status in the vitreous body of eyes, which had been vitrectomized due to rhegmatogenous retinal detachment (RRD) with or without proliferative vitreoretinopathy (PVR), is higher than in eyes vitrectomized due to other retinal diseases. (2) Methods: four patient groups were analyzed: 22 eyes of patients with RRD without PVR, 27 eyes with RRD and PVR, 22 eyes with macular hole (MH) and 10 eyes with epiretinal membrane (ERM). Spectrophotometric methods were used to determine the total antioxidant status (TAS) values as well as superoxide dismutase (SOD) and glutathione reductase (GR) activities in the vitreous fluid samples. (3) Results: no significant differences in TAS values and antioxidant enzyme activities were observed among patient with RRD with and without PVR and with MH and ERM. The longer the duration of RRD leading to PVR and better postoperative visual acuity, the higher the TAS level. No significant differences were found between “macula on” and “macula off” subgroups within the RRD group and the RRD combined with PVR group. (4) Conclusions: The preliminary results do not support the thesis that the antioxidant status of vitrectomized eyes is different in patients with RRD with or without PVR in comparison to patients with MH and ERM. In patients with RRD, PVR presence and detached macula do not affect the values of TAS, SOD and GR in the vitreous fluid. The duration of the disease influences TAS in the vitreous in eyes with RRD complicated with PVR.
Multi-Gene Phylogeny and Morphology Reveal Haplohelminthosporium gen. nov. and Helminthosporiella gen. nov. Associated with Palms in Thailand and A Checklist for Helminthosporium Reported Worldwide
Authors: Konta Sirinapa , Hyde Kevin D. , Karunarathna Samantha C. , Mapook Ausana , Senwanna Chanokned , Dauner Lucas A. P. , Nanayakkara Chandrika M. , Xu Jianchu , Tibpromma Saowaluck , Lumyong Saisamorn
Published: May 2021

Palms (Arecaceae) are substrates for a highly diverse range of fungi. Many species are known as saprobes and many are important plant pathogens. Over the course of our studies of micro-fungi from palms in Thailand, two new taxa were discovered. Morphological characteristics and phylogenetic analyses of combined ITS, LSU, SSU, and tef1-α sequence data revealed their taxonomic positions within Massarinaceae. There are currently ten genera identified and accepted in Massarinaceae, with the addition of the two new genera of Haplohelminthosporium and Helminthosporiella, that are introduced in this paper. Each new genus is provided with a full description and notes, and each new taxon is provided with an illustration for the holotype. A list of identified and accepted species of Helminthosporium with morphology, host information, locality, sequence data, and related references of Helminthosporium reported worldwide is provided based on records in Species Fungorum 2021. This work provides a micro-fungi database of Haplohelminthosporium, Helminthosporiella, and Helminthosporium which can be modified and validated as new data come to light.
Neutralization of Acidic Wastewater from a Steel Plant by Using CaO-Containing Waste Materials from Pulp and Paper Industries
Authors: Jarnerud Tova , Karasev Andrey V. , Jönsson Pär G.
Published: May 2021

In this study, CaO-containing wastes from pulp and paper industries such as fly ash (FA) and calcined lime mud (LM) were utilized to neutralize and purify acidic wastewaters from the pickling processes in steel mills. The investigations were conducted by laboratory scale trials using four different batches of wastewaters and additions of two types of CaO-containing waste materials. Primary lime (PL), which is usually used for the neutralization, was also tested in the same experimental set up in the sake of comparison. The results show that these secondary lime sources can effectively increase the pH of the acidic wastewaters as good as the commonly used primary lime. Therefore, these secondary lime sources could be potential candidates for application in neutralization processes of industrial acidic wastewater treatment. Moreover, concentrations of metals (such as Cr, Fe, Ni, Mo and Zn) can decrease dramatically after neutralization by using secondary lime. The LM has a purification effect from the given metals, similar to the PL. Application of fly ash and calcined lime mud as neutralizing agents can reduce the amount of waste from pulp and paper mills sent to landfill and decrease the need for nature lime materials in the steel industry.
The Mould War: Developing an Armamentarium against Fungal Pathogens Utilising Thymoquinone, Ocimene, and Miramistin within Bacterial Cellulose Matrices
Authors: Swingler Sam , Gupta Abhishek , Gibson Hazel , Heaselgrave Wayne , Kowalczuk Marek , Adamus Grazyna , Radecka Iza
Published: May 2021

An increase in antifungal resistance has seen a surge in fungal wound infections in patients who are immunocompromised resulting from chemotherapy, disease, and burns. Human pathogenic fungi are increasingly becoming resistant to a sparse repertoire of existing antifungal drugs, which has given rise to the need to develop novel treatments for potentially lethal infections. Bacterial cellulose (BC) produced by Gluconacetobacter xylinus has been shown to possess many properties that make it innately useful as a next-generation biopolymer to be utilised as a wound dressing. The current study demonstrates the creation of a pharmacologically active wound dressing by loading antifungal agents into a biopolymer hydrogel to produce a novel wound dressing. Amphotericin B is known to be highly hepatotoxic, which reduces its appeal as an antifungal drug, especially in patients who are immunocompromised. This, coupled with an increase in antifungal resistance, has seen a surge in fungal wound infections in patients who are immunodeficient due to chemotherapy, disease, or injury. Antifungal activity was conducted via Clinical & Laboratory Standards Institute (CLSI) M27, M38, M44, and M51 against Candida auris, Candida albicans, Aspergillus fumigatus, and Aspergillus niger. This study showed that thymoquinone has a comparable antifungal activity to amphotericin B with mean zones of inhibition of 21.425 ± 0.925 mm and 22.53 ± 0.969 mm, respectively. However, the mean survival rate of HEp-2 cells when treated with 50 mg/L amphotericin B was 29.25 ± 0.854% compared to 71.25 ± 1.797% when treated with 50 mg/L thymoquinone. Following cytotoxicity assays against HEp-2 cells, thymoquinone showed a 71.25 ± 3.594% cell survival, whereas amphotericin B had a mean cell survival rate of 29.25 ± 1.708%. The purpose of this study was to compare the efficacy of thymoquinone, ocimene, and miramistin against amphotericin B in the application of novel antifungal dressings.
Effects of Corrosion on Compressive Arch Action and Catenary Action of RC Frames to Resist Progressive Collapse Based on Numerical Analysis
Authors: Zhang Lu , Wei Tingyu , Li Hongyu , Zeng Jian , Deng Xiaofang
Published: May 2021

Many negative factors can influence the progressive collapse resistance of reinforced concrete (RC) frame structures. One of the most important factors is the corrosion of rebar within the structure. With increasing severity of corrosion, the duration, robustness, and mechanical performance can be greatly impaired. One specific side effect of rebar corrosion is the significant loss of protection against progressive collapse. In order to quantify the effects of rebar corrosion on load-resisting mechanisms (compressive arch action (CAA) and tensile catenary action (TCA)) of RC frames, a series of numerical investigations were carried out in this paper. The previous experimental results reported in the literature provide a benchmark for progressive collapse behavior as a sound condition and validate the proposed numerical model. Furthermore, based on the verified numerical model, the CAA and TCA with increasing corrosion and an elapsed time from 0 to 70 years are investigated. Comparing with the conventional empirical model, the proposed numerical model has shown the ability and feasibility in predicting the collapse resistance capacity in structures with corroded rebar. Therefore, this numerical modeling strategy provides comprehensive insights into the change of load-resisting mechanisms in these structures, which can be beneficial for optimizing the design.
Effects of the Replacement of Co with Ni on the Microstructure, Mechanical Properties, and Age Hardening of AlCo1−xCrFeNi1+x High-Entropy Alloys
Authors: Lee Che-Fu , Shun Tao-Tsung
Published: May 2021

In this study, effects of the replacement of Co with Ni on the microstructure, mechanical properties, and age hardening of high-entropy alloys of AlCo1−xCrFeNi1+x (x = molar ratio; x = 0, 0.5, 1, denoted as X0, X0.5, and X1, respectively) were investigated. These three alloys exhibited a dendritic structure comprising an ordered BCC matrix, a BCC phase, and an FCC or an ordered FCC phase. From X0 to X1 alloys, the yield stress and compressive stress decreased from 1202 and 1790 MPa to 693 and 1537 MPa, respectively. However, fracture strain increased from 0.15 to 0.42. Peak age hardening at 600 °C for the X0 alloy was due to the precipitation of the (Cr,Fe)-rich σ phase. Peak age hardening for the X0.5 and X1 alloys was observed at 500 °C because of the precipitation of the σ phase and BCC phase, respectively.