bluuebunny/crossref_metadata_2025 huggingface dataset:
THE EFFECT OF MICRONUTRIENTS ON POTATO TUBERS COLONIZED BY NEMATODES DITYLENCHUS DESTRUCTOR IN ARTIFICIAL INFECTION
Authors: Lychagina S. V. , Zakharova V. V.
Published: May 2024

Potatoes in our country are a vegetable crop of strategic importance. The merit of
this vegetable is in its multifaceted use. But there are no potato varieties resistant
to the potato tuber nematode yet. The potential is in biological and biochemical
characteristics of varieties in addition to various mineral nutrition. The experiment
studied the effect of various micronutrients on increasing the resistance of Gala
potato plants to phytoparasitic nematodes Ditylenchus destructor during artificial
infection of the apical tuber segments. Standard micronutrient chelated solutions
(B10,1%K2
O44%; Ca10%; Cu15%; Fe11%; Mn13%; Zn15%) for experiments were prepared
in concentrations as recommended by the manufacturer. Shallow scalpel incisions
were made on the surface of each segment and a Ditylenchus destructor suspension
was instilled into the incision. The segments wetted with the standard solutions
were put in plastic lidded containers to preserve humidity. The variants were labeled
and left for 20 days. The control variant was sprayed with clean water. At 20 days
that were enough for nematode colonization in potato slices, the tuber segments
and nematode populations therein were examined. The result of the Ditylenchus
destructor development in the potato segments with the use of micronutrients showed
that the least nematodes were observed in the variants treated with copper chelate
(EDTA-Cu15%) and iron chelate (Fe11%). Slightly more of them were in the variant with potassium metaborate (B10,1%K2
O44%). The remaining experiment variants and
the control variant had the largest number of nematodes and their larvae.
PARASITE FAUNA BIODIVERSITY IN ANIMALS AND PLANTS IN ANTHROPOGENIC ECOSYSTEM OF THE NORTH ARMENIAN REGIONS, LORI AND TAVUSH, AND THE LAKE SEVAN BASIN
Authors: Movsesyan S. O. , Vardanyan M. V. , Petrosyan R. A. , Nikogosyan M. A. , Arutyunova L. D. , Rukhkyan M. Y. , Hovhannisyan R. L. , Akopyan K. V. , Barseghyan R. E. , Dudukchyan Z. M. , Terenina N. B. , Voronin M. V.
Published: May 2024

The parasite fauna in domestic animals, birds, fish, ixodofauna, some biohelminths’
circulation routes, and nematodes isolated from wood samples were studied. The
fauna includes 50 species including 29 helminth species, of which 6 trematode
species: Dicrocoelium dendriticum (D. lanceatum), Fasciola hepatica, F. gigantica,
Paramphistomum cervi, Diplostomum spathaceum and D. paraspathaceum; 4 cestode
species: Moniezia expansa, M. benedeni, Ligula intestinalis and Schyzocotyle
acheilognathi; 18 nematode species: Ascaris suum, Ascaridia galli, Amidostomum
anseris, Heterakis gallinarum, Syngamus trachea, Capillaria obsignata, Trichuris
ovis, T. suis, Chabertia ovina, Nematodirus spathiger, Protostrongylus hobmaieri,
Muellerius capillaris, Cystocaulus nigrescens, Metastrongylus elongatus, Strongylus
equinus, Passalurus ambiguus, Rhabdochona macrostoma and R. fortunatovi, and one
acanthocephalan species Pomphorhynchus kostylewi. We also found seven Eimeria
species: E. exigua, E. media, E. stiedae, E. irresidua, E. perforans, E. magna and
E. tenella, and two haematophagous parasites: Babesia bigemina and B. canis. The
terrestrial molluscs, intermediate hosts of Dicrocelia and Protostrongylus, were
represented by the species Hesseola solidior, and freshwater molluscs, intermediate
hosts of Dicrocelia and paramphistomes, were represented by two species: Ancylus
fluviatilis and Planorbis planorbis. One species of soil mites, intermediate hosts of
cestodes of the Scheloribates gen., was identified. We identified species composition
of ixodid ticks totaling 12 species: Ixodes ricinus, I. redikorzevi, I. persulcatus,
Dermacentor marginatus, D. pictus, D. silvarum, Hyalomma aegyptium, H. marginatum
(= H. plumbeum), H. asiaticum caucasicum, Rhipicephalus sanguineus, R. annulatus,
R. bursa.
HELMINTHOZOONOSIS AND TOXOPLASMOSIS IN THE RYAZAN REGION
Authors: Novak M. D. , Novak A. I. , Kleimenova Y. Y.
Published: May 2024

Laboratory tests were carried out for toxocariasis, strongyloidiasis, and ancylostomiasis
of carnivorous animals and a statistical epidemiological record was made for the
prevalence of toxoplasmosis. Fecal samples from dogs were found to have eggs of
Strongyloides stercoralisc and Ancylostoma caninum using the flotation method in
eight cases out of ten (prevalence = 80%, infection intensitymean = 26 specimens)
in the Ryazan Central Park, and in five cases of ten (prevalence = 50%, infection
intensitymean = 12 specimens) in the Novopavlovskaya Wood. Using the BermanOrlov method, nematode larval stages were identified in all positive samples: the
mean invasion intensity was 38 specimens in one sample in the Central Park, and 26
specimens in the Novopavlovskaya Wood. The method of cultivation in a thermostat
found Strongyloides stercoralis and Ancylostoma caninum larvae in quantities of
39 to 45 specimens. In one of the shelters in Ryazan, the diagnosis of toxocariasis
was confirmed in 7%, ancylostomiasis in 9%, and strongyloidiasis in 4,5% when
examining puppies. A retrospective analysis for toxocariasis in dogs (puppies aged
1.5–3.5 months) showed the following results: 13.2% in 2012, 17.8% in 2015, 15.5%
in 2018, 18.1% in 2021, and 14.6% in 2023. According to the Center for Hygiene
and Epidemiology of the Ryazan Region, seropositive results for toxoplasmosis were
detected in 58 cases with a subclinical, latent course of the disease for 1998 to 2021.
RISKS OF SOIL BIOCONTAMINATION OF THE RUSSIAN FEDERATION WITH THE INTRODUCTION OF A DANGEROUS PARASITE, THE PALE POTATO CYST NEMATODE GLOBODERA PALLIDA
Authors: Perevertin K. A. , Vasiliev T. A. , Perevertina J. K. , Burnashev М. R.
Published: May 2024

According to the classification of agroecological soil assessment, agrobiocenosis
contamination with the pale potato cyst nematode Globodera pallida is irreversible
(other than, for example, the potato tuber nematode Ditylenchus destructor
biocontamination that is reversible), which once again underlines the importance of quarantine control. A very disturbing fact is the presence of this dangerous parasite in
the countries of Europe and North America, although their agricultural technologies
including plant protection are at a very high level. The spread of this cyst-forming
nematode depends entirely on anthropogenic factors since the possibility of active
spread of second instars is almost negligible (several tens of centimeters for the
growing season). The worldwide nematode spreading from the historical South
American gene center of origin of cultivated plants (where the origin is of the
potato with which the nematode is co-evolutionarily associated) had a complex
nature primarily determined by such a factor as centuries-old practice of selective
improvement of potato varieties in Europe. The Institute of Plant Quarantine has
developed a computer risk assessment system for the inspection of quarantined
cargoes including potatoes, which makes it possible to organize the inspection of
seed and commercial potatoes more effectively from the countries where the pale
potato cyst nematode is recorded.
OVOCIDAL PROPERTIES OF SAPONIN DAB 9/10
Authors: Postevoy A. N. , Andreyanov O. N. , Puzanova E. V.
Published: May 2024

The research purpose was to study the ovocidal properties of commercial Saponin
DAB 9/10. Its standard aqueous solutions were studied. The test object was eggs of
the nematode Toxocara canis. They were obtained from gonads of fertilized female
Toxocara nematodes isolated from carnivores. Laboratory studies were conducted
according to the Disinfectant Testing Guidelines in Veterinary Medicine. After contact
with freshly isolated helminth eggs, the egg suspension was incubated in a thermostat
at 28±2 оC. One-hour aeration was performed daily in petri dishes with nematode
eggs. Stage 1 larvae were monitored for 2 weeks. At day 21, mechanical exposure
was produced on shells of helminth eggs and a biological sample was evaluated on
laboratory mice. No effective ovocidal action was observed on Toxocara eggs under the
effect of different concentrations of Saponin DAB 9/10. The experimental helminth
eggs developed unchanged at such time as the control eggs. A biological test on the
laboratory mice was positive. Saponin DAB 9/10 did not provide any desired effect for
disinvasion against the causal agent of toxocariasis T. canis.
COMPARATIVE EFFICACY OF DIFFERENT DICLAZURIL
DOSAGE FORMS AGAINST SPONTANEOUS COCCIDIOSIS
IN BROILER CHICKENS
Authors: Safiullin R. T. , Chalysheva E. I. , Tashbulatov A. A.
Published: May 2024

Our research conducted in previous years has shown that coccidiosis of floor
management broiler chickens is widespread on commercial poultry farms in
Russia. Many remedies have been proposed to control the infection, among which
Diclazuril should be noted. The research objective was to test the efficacy of different
doses of Diclazuril and the prescribing scheme of the drug with feed and drinking
water against spontaneous coccidiosis in broiler chickens. For the test, 500 floor
management chickens were selected among those spontaneously infected with
coccidia and divided into 5 similar groups. Group 1 chickens were prescribed 2.5%
Diclazuril with drinking water in a dosage form of 2 mL per 1 L of water, without
interruption, for 48 hours. Group 3 chickens received 30% Amprolium at a dose of 0.4 g per 1 L of water for 48 hours. The indicated Diclazuril and Amprolium doses
were prescribed to the chickens of groups 1 and 3 in three stages: at 9–10, 16–17 and
24–25 days of age. Diclazuril and Amprolium were administered to the chickens of
the second and fourth groups with feed at 10 to 38 days of age. The chickens of group
5 did not receive drugs and served as infected controls. The efficacy of different
Diclazuril doses compared with the base drug Amprolium was determined by the
results of coproscopic examinations of the chickens at the age of 30 days.
The results obtained in testing different diclazuril dosage forms showed that the
best intense-effectiveness was observed in the prescribed dose of 2 mL per 1 L of
drinking water for 48 hours according to the said scheme and it was 94.1%. The
recommended dose of Amprolium provided 84.9% intense-effectiveness against
coccidiosis in broiler chickens.
EFFECTIVENESS OF JOINT ADMINISTRATION OF AN ANTICOCCIDIAL DRUG AND A BIOGENIC STIMULANT AGAINST SPONTANEOUS COCCIDIOSIS OF BROILERS IN A FARM SCALE TRIAL
Authors: Safiullin R. T. , Shibitov S. К.
Published: May 2024

The research conducted in our country and abroad has proven that any poultry farm
that practices poultry floor management is affected by parasitic diseases, especially
eimeriosis.
The purpose of this study was to test the effectiveness of joint administration of the
anticoccidial drug Diclazuril and the biogenic stimulant ASD against spontaneous
coccidiosis in broiler chickens under production conditions. The effectiveness of
joint administration of Diclazuril was tested on 1,739 floor management chickens
aged 9 to 38 days, at a dose of 2 mL per 1 L of drinking water for 48 hours according to
the following scheme: on day of age 9–10; 16–17 and 24–25, and the biogenic stimulant ASD in a dose of 20 mL per 1 L of drinking water for 72 hours according to the
scheme: on day of age 9–11; 15–17 and 23–25. The control chickens were given the
recommended dose of Amprolium with water. The prevalence and intensity of Coccidia infection, as well as the intense-effectiveness of joint administration were determined based on results of coproscopic examinations of 30 litter samples from each
group. The joint administration effect on the weight gain of the broilers was studied
on 60 chickens that were individually tagged and weighed at 9 and 38 days of age.
The farm scale trial results showed that the intense-effectiveness of the joint administration of Diclazuril and the biogenic stimulant ASD against spontaneous coccidiosis in the broiler chickens was 93.5%, and this had a positive effect on the productivity of the broilers that had their average daily weight gained by 11.5% compared
to the control group.
MORPHOMETRY OF CAPILLARIA EGGS IN THE MALLARD ANAS PLATYRHYNCHOS (LINNAEUS, 1758) IN PERM
Authors: Sivkova T. N. , Khazova M. V.
Published: May 2024

Wild waterfowl are a natural reservoir of helminths and put domestic ducks, geese
and some other species at risk of infection; however, studies on the spread of
endoparasites among them were conducted rarely in the Perm Territory. Despite
successes in gene diagnostics, helminth species determination by morphological
characteristics remains the basic approach. We took 51 fecal samples from the
mallard Anas platyrhynchos (Linnaeus, 1758) that winters in Perm. Such samples
were studied in the Laboratory of Parasitology, the Department of Veterinary
Medicine and Animal Technologies, the Perm State Agro-Technological University.
We used the method of G. A. Kotelnikov and V. M. Khrenov with an ammonium
nitrate solution, and sequential washing. The egg morphometric analysis was
performed using the PhotoM 1.21 software (Russia). Among the total number of
samples, eggs with Capillaria sp. morphology were found in 21 samples (prevalence,
41.18%). All identified eggs had the same parameters in size and length & width ratio,
which indicates they belonged to one species. According to literature descriptions,
they correspond to eggs of Capillaria (Eucoleus) annulata (Molin, 1858), which is
potentially dangerous to poultry.
STUDY ON POTENTIAL IMMUNOREGULATORY PROTEINS IN THE EXCRETORY-SECRETORY PRODUCTS OF CESTODES
Authors: Sodnomov T. C. , Kutyrev I. A.
Published: May 2024

This article studied excretory-secretory products of parasitic flatworms aimed at
searching for potential immunoregulatory proteins. Immunoregulatory proteins
are poorly studied at the moment. Recent years have showed increased interest in
identifying immunoregulatory molecules produced by parasitic worms. Potential
immunoregulatory proteins will make a significant contribution to the development
of medicine and biotechnology and will make it possible to effectively treat allergic
and other autoimmune diseases. The study used methods of bioinformatics,
proteomics, and transcriptomics. Potential immunoregulatory proteins were
identified in the Ligula interrupta secretome proteins and listed. Each protein was
analyzed for possible immunoregulatory functions in the parasite-host system. Based
on identification data of SEP proteins using parasite transcriptomes, annotation
of secretome proteins (SEP) was made in the NCBI and Swissprot international
databases. A table was compiled with identified potential immunoregulatory
proteins. A functional analysis of each protein was performed. Protein functions
were determined based on analysis of scientific articles, patents and publications. By
comparing different proteins, it is possible to identify those that are similar in domain
structure, phylogeny, and description. The discovered potential immunoregulatory
proteins will make a significant contribution to the development of medicine and biotechnology and will make it possible to effectively treat allergic and other
autoimmune diseases.
APPLICATION OF THE PCR METHOD TO DETECT DNA OF THE CAUSATIVE AGENT OF CYSTIC ECHINOCOCCOSIS IN THE BLOOD
Authors: Telicheva V. O. , Nagorniy S. A. , Ermakova L. A. , Golovchenko N. V. , Kornienko I. V. , Kirtanasova E. I.
Published: May 2024

Echinococcosis remains a complex multidisciplinary problem. Due to frequent
intra- and postoperative complications, and distant recurrence of echinococcosis,
the successful treatment of the patient depends on interaction of doctors in surgery
and therapeutics. To date, there has been no consensus in the Russian Federation on
management tactics of echinococcosis patients. There are no protocols or clinical
guidelines to choose a surgical intervention volume and method or the duration of
anti-relapse treatment courses. Currently, the main laboratory method to evaluate
the effectiveness of surgical and anti-relapse treatment is enzyme immunoassay
(ELISA) to detect IgG responses to unilocular echinococcus antigens. During cyst
growth, antibody titers can vary greatly and initiate cross-reactions. Therefore,
a search remains relevant for diagnostic methods that allow assessing infection
recurrence risks to determine the anthelmintic therapy duration. The PCR method
using our developed primer pairs, isolation conditions, and the amplification
mode, studied hydatid cyst fluid obtained by the PAIR with anaphylactic shock as a
complication, as well as matched blood samples of the patient taken within 12 hours
after anaphylactic shock and a month after anthelmintic therapy. Echinococcalspecific DNA was found by PCR in the cyst contents and the blood samples of
the patient obtained during the surgical intervention and a month after the antirelapse treatment. The molecular genetic research method can serve as a tool in
the cyst echinococcosis diagnosis as an additional method to the histological one.
Contamination of blood and other cavities and organs during the intervention is of
great importance for determining a further management tactics of echinococcosis
patients.
PRIMARY STAGE OF TOXOCARA ANTIGEN PRODUCTION
Authors: Andreyanov O. N. , Uspensky A. V. , Postevoy A. N.
Published: May 2024

The article describes a method for isolating Toxocara canis larvae from chorions.
Nematode larvae can serve as a specific antigen in the diagnosis with serological
reactions. The study material was cultures of Toxocara eggs obtained from female
nematode gonads. Toxocara eggs were stored in a 4.0% formalin solution or a
1.0% hydrochloric acid solution. Sexually mature female Toxocara species were
obtained by isolating helminths from the intestines of carcasses of naturally infected
carnivores. For artificial isolation of Toxocara larvae from eggs, a method of their
short-term incubation in the bile of ruminants was used. The nematode egg culture
was put on a heated magnetic stirrer. A magnet in plastic of 1.5–3.5 cm was placed
in a sample, and heating to 38±1 оC was turned on. After 30-minute exposure, the
larvae were washed in the saline. For convenience, they may be placed in a Berman
device at 38±1 оС. The materials freshly obtained and stored for 3 months showed a
high percentage of larval yield from eggs. The stored material showed a rather worse
result for the 1st year.
INFECTION OF THE EUROPEAN VENDACE COREGONUS
ALBULA (LINNAEUS, 1758) FROM LAKE SEGOZERO BY
MYXOSPORIDIUM HENNEGUYA ZSCHOKKEI (GURLEY, 1894)
Authors: Farhat A. A. , Avvakumova O. V. , Malofeeva N. A.
Published: May 2024

The article presents study data of the myxosporidium Henneguya zschokkei
infection in the European vendace in Lake Segozero. The lake is located in the
Medvezhyegorsk and Segezha Districts of the Republic of Karelia and belongs to
the White Sea basin. Ichthyological material was collected using passive fishing gear
(nets). The material was collected and processed according to the common method
of partial parasitological dissection. The study was carried out from November 2023
to February 2024 in the Laboratory of Biology and Biological Basis of Preventive Measures of the VNIIP – FSC VIEV. Forty specimens of the European vendace
Coregonus albula (Linnaeus, 1758) were taken from Lake Segozero, the Republic
of Karelia, for the study. Myxosporidia Henneguya zschokkei (Gurley, 1894) were
found in the muscle tissue of the studied fish samples. Myxosporidium Henneguya
zschokkei is not dangerous for human and animal health but can significantly reduce
the fish value since myxosporidium cysts localize in skeletal muscles and are clearly
visible to the naked eye without optical means, and negatively affect the market
condition of fish. The results showed the need for such studies during veterinary and
sanitary assessment of safety and quality of fish in helminth infections.
DIALYSIS STUDY OF TRICLABENDAZOLE-BASED DRUGS
Authors: Khalikov M. S. , Ilyin M. M.
Published: May 2024

The research purpose was to study the dialysis of triclabendazole (TCB) and its solid
dispersions (SDs) obtained by mechanochemical methods by modifying TCB with
polyvinylpyrrolidone (PVP), arabinogalactan (AG) and succinic acid (SA).
Solid dispersions of the following compositions were obtained: TCB:AG:PVP
(1:4,5:4,5), TCB:SA:AG (1:1:8) and TCB:SA:PVP (1:1:8), which were characterized
by their solubility in water. To study the TCB release from the obtained SDs
(dialysis), we used a laboratory installation consisting of a thermostated glass with
a buffered solution (sodium borate) with pH 9.18 (intestinal environment). The
TCB substance concentration in the dialysate was determined by HPLC the data
of which determined that the TCB release into the buffered solution at different
rates depended on the SD composition. It was found that SA included in the SD
composition significantly increased the TCB release rate, apparently due to the
buildup of a salt form from TCB (weak base) and SA (weak acid).
The following conclusions were drawn: the increase in the TCB solubility in the
studied SDs, as well as the increase in the rate of the TCB release from the SDs
with succinic acid was consistent with previously obtained data on high antiparasitic
activity of these SDs in sheep fascioliasis.
THE EFFECT OF ENVIRONMENTAL FACTORS OF THE MOUNTAIN BELT ON THE LARVAL DEVELOPMENT OF DICTYOCAULUS FILARIA (RUD., 1809) AND MONIEZIA EXPANSA (RUD., 1810)
Authors: Ataev A. M. , Zubairova M. M. , Karsakov N. T , Kataeva D. G.
Published: May 2024

In the high-altitude aspect of the mountain belt, a negative effect is observed for
physical environmental factors on the development of helminth larval stages.
In particular, it should be noted that there are differences between daytime and
nighttime temperatures. In summer, during the day in the mountain zone, the air
warms up to +25 оC, and at night the temperature drops to 0 оC and sometimes lower. Severe changes in temperature in mountains are one of the main factors affecting
the development of the infective principle of helminth larval stages in external
environment. We experimentally studied the effect environmental factors had on
the larval development of Dictyocaulus filaria (Rud., 1809) and Moniezia expansa
(Rud., 1810) in the mountain belt. At the same time, it was found that up to 46.0%
of infective D. filaria larvae overwintered in external environment at an altitude of
1200 m above sea level, and respectively their survival rate in the trachea and bronchi
of 3-month-old lambs with artificial infection was 34.1%. By spring, up to 39.6%
of oribatid mites infected with M. expansa cysticercoids overwintered at an altitude
of 1200 m above sea level, that survived in 23.3 to 30,0% in lambs, accordingly. The
survival rate of M. expansa – free oribatid mites was 77.8%.
The survival rate of D. filaria larvae that overwintered in external environment up to
2500 m above sea level reached 18.5%.
Of infective larvae, D. filaria survived from 1 to 2.0% by spring at an altitude of 2500
m above sea level, and non-infective D. filaria larvae died, accordingly.
OPISTHORCHIDAE INFECTION IN COMMERCIAL FISH
IN THE OB RIVER AND THE NOVOSIBIRSK RESERVOIR
Authors: Bonina O. M. , Efremova E. A. , Zubareva I. M. , Samokhina E. R. , Brem A. K.
Published: May 2024

The article presents data on Opisthorchidae metacercariae infection in commercial
fish of the carp family from two water bodies, the Novosibirsk Reservoir and the Ob
River in the Novosibirsk Region. Opisthorchidae metacercariae were identified in fish
muscles using the compression method. The prevalence, the invasion intensity and an
abundance index were calculated. The ide, the bream and the roach were studied. The
fish were infected by O. felineus, М. bilis and М. xanthosomus metacercariae. In the
Ob River, the average prevalence of O. felineus metacercariae in the ide was 69.1%, M.
bilis metacercariae, 27%, and M. xanthosomus metacercariae, 17.3%; in the Reservoir,
these values were 17.5, 4.8 and 0.6% in the ide, respectively. The River breams were
found to be uninfected, and the Reservoir breams had the prevalence of O. felineus, M. bilis and M. xanthosomus metacercariae of 4.4, 7.2 and 1.1%, respectively. The
O. felineus metacercariae infection intensity in ides from the River was significantly
higher than that the Reservoir. The roach rarely reaches large sizes and is therefore
not often found in commercial catches. As a source of Opisthorchidae, it poses much
greater danger to domestic and wild carnivores. The Ob River ide is most dangerous
to humans from the point of view of infection with Opisthorchidae larval forms.
HELMINTH FAUNA OF THE CRUCIAN CARP (CARASSIUS CARASSIUS) AND SILVER PRUSSIAN CARP (CARASSIUS GIBELIO) IN WATER BODIES OF THE REPUBLIC OF BELARUS
Authors: Bychkova Е. I. , Yakovich М. М. , Degtyarik S. М.
Published: May 2024

Currently, an alien fish species, the silver Prussian carp Carassius gibelio is
widespread in almost all reservoirs of Belarus due to its high competitiveness. The
introduction of silver Prussian carps in commercial reservoirs of the Republic
caused significant replacement of the native species, the crucian carp. Potentially,
alien helminth species introduced in water bodies by the silver Prussian carp have
affected the crucian carp population. The helminth fauna of the silver Prussian carp
in Belarussian water bodies is represented by 16 species: 1 nematode species (genus
Philometroides Yamaguti, 1935), 2 cestode species (1 of the genus Neogryporhynchus
Baeret Bona, 1960, and 1 of the genus Khawia Hsu, 1935); 6 monogeneas (4 of the genus Dactylogyrus Diesing, 1850; 1 of the genus Gyrodactylus Nordmann, 1832,
and 1 of the genus Diplozoon Nordmann, 1832); 7 trematode species (1 of the genus
Rhipidocotyle Diesing, 1858, 2 of the genus Posthodiplostomum Dubois, 1936, 1 of the
genus Diplostomum Nordmann, 1832, 1 of the genus Ichthyocotylurus Odening, 1969,
1 of the genus Paracoenogonimus Katsurada, 1914, and 1 of the genus Tylodelphys
Diesing, 1850). The helminth fauna of the crucian carp is represented by 13 species:
1 acanthocephalan species (genus Acanthocephalus Koelreuter, 1771); 1 nematode
species (genus Camallanus Railliet et Henry, 1915); 3 cestode species (1 of the genus
Caryophyllaeus Gmelin, 1790, and 2 of the genus Khawia Hsu, 1935); 3 monogeneas
(1 of the genus Dactylogyrus Diesing, 1850, 1 of the genus Gyrodactylus Nordmann,
1832, and 1 of the genus Diplozoon Nordmann, 1832); and 5 trematode species (1 of
the genus Echinochasmus Dietz, 1909, 1 of the genus Paracoenogonimus Katsurada,
1914, 1 of the genus Tylodelphys Diesing, 1850, 1 of the genus Allocreadium Looss,
1900, and 1 of the genus Phyllodistomum Braun, 1899). The helminth fauna had low
similarity (4 species) in the silver Prussian carp when compared with the crucian carp.
Comparison of Intraoperative and Postoperative Bleeding Risks Between Robot-assisted and Laparoscopic Hysterectomy
Authors: Kim Tae Yeong , Park So Hee , Sang Jae Hong , Choi Jeong In , Chung Soo-Ho
Published: May 2024

Background: This study aimed to compare bleeding risks between robot-assisted and laparoscopic hysterectomy and to provide a guidance for selection of surgical methods to minimize intraoperative bleeding. Methods: We enrolled patients who underwent robotic or laparoscopic hysterectomy at our institution from January 2021 to December 2022. We compared the percentage decrease in hemoglobin levels from within 1 month prior to surgery to postoperative days 1 and 3; the total Jackson-Pratt (JP) drainage by postoperative day 2 or 3; input/output (I/O) difference which means the subtraction of all output including urine and JP drainage volume from the total input of administered fluids, which implies amounts of blood lost on the day of surgery; and the numbers of patients who required transfusions. We compared patients who underwent robotic and laparoscopic hysterectomy, and subgroups thereof based on the uterine weight and the extent of adhesiolysis. Analyses employed IBM SPSS software. The independent samples t-test was used to compare the various groups. A p-value less than 0.05 was considered statistically significant. Results: Robotic hysterectomy demonstrated superior outcomes in terms of the total JP drainage compared to laparoscopic hysterectomy. However, no significant differences were demonstrated between the two surgical procedures in terms of the percentage decrease in hemoglobin levels from 1 month prior to surgery to postoperative days 1 or 3, or I/O difference on the day of surgery. Notably, only patients who received laparoscopic hysterectomy required blood transfusions. Conclusions: Our results support the hypothesis that robotic hysterectomy is not only similar, but actually superior to laparoscopic hysterectomy in terms of bleeding control. Our findings offer valuable guidance when decisions are made to choose surgical method via collaborative consultations involving a medical team and a patient. In particular, our results are beneficial for patients seeking to minimize bleeding during surgery and for those who are unfamiliar with the differences between robotic and laparoscopic hysterectomy.
Sexual Health among Thai Women with Gynecological Problems at a University Hospital
Authors: Muakkeaw Thanyada , Sumdaengrit Bualuang , Pokpalagon Piyawan
Published: May 2024

Background: Sexual health is one part of quality of life for women. Those who have gynecological problems may find their sexual health problem, and consequently their quality of life, negatively affected. Thus, the objectives of this study were to: (1) study the sexual health of women with gynecological problems; and (2) compare the sexual health of women with different gynecological problems. Methods: This is a descriptive-comparative study design. Sexual health conceptualization by the World Health Organization was used as a conceptual framework. Data were collected from Thai women with gynecological problems, age 20–59 years, who were actively engaging in sexual intercourse, and who underwent examination at the gynecological outpatient department of a university hospital. Two questionnaires were used: (1) a personal and health status information collection document; and (2) a sexual health questionnaire. The data were analyzed using descriptive statistics, independent t-test, and Mann-Whitney U test in the cases of data that did not meet the assumptions. Results: Two hundred and two women were approached, and 140 consented to participate (69.3% response rate). The results revealed that the age of the participants ranged from 21 to 54 years, with a mean of 36.9 years (standard deviation (SD) = 8.1). The mean score of sexual health problems was 37.3 (SD = 12.1); the score ranged between 0 and 114, with reports of mild (1–38) and moderate/high levels of sexual health problems (>38). In addition, there was a difference in significance between women who had dyspareunia and those who did not (p < 0.000), and between women with paleness/fatigue and those who did not display symptoms (p = 0.004). However, there was no significant difference in sexual health problems between women who suffered abnormal uterine bleeding, dysmenorrhea, or chronic pelvic pain and those who did not. Conclusions: The results of this study can be used as a guideline for providing nursing care to women with gynecological problems, covering all dimensions of sexual health, including reducing other abnormalities and various pains in order to promote sexual health among these women.
miRNA-27b-3p, let-7f-5p and miRNA-142-5p can be Used in a Novel Serum Diagnostic Panel for Clear Cell Renal Cell Carcinoma
Authors: He Tao , Lu Chong , Gong Wei , Ge Zhenjian , Li Rongkang , Li Xinji , Sun Chen , Li Wentao , Long Qishan , Liu Qiang , Lai Yongqing
Published: May 2024

Background: Clear cell renal cell carcinoma (ccRCC) is a prevalent malignant tumor affecting the urinary system. Due to its unfavorable prognosis, there is a pressing need to discover effective approaches for early diagnosis and treatment of ccRCC. Extensive research has consistently demonstrated the presence of stable microRNAs (miRNAs) in human serum. Accordingly, the objective of this study was to identify a specific panel of miRNAs in serum that can serve as a reliable and non-invasive biomarker for the early detection of ccRCC. Methods: The study comprised of training and validation phases to identify potential biomarkers. In the training phase, a total of 10 miRNAs exhibiting the most significant differential expression among 28 ccRCC patients and 28 healthy controls (HCs) were identified using quantitative reverse transcription polymerase chain reaction (qRT-PCR). In the subsequent validation phase, these 10 miRNAs were assessed in serum samples obtained from an additional 80 ccRCC patients and 84 HCs using RT-qPCR. To construct a panel with optimal diagnostic capability, backward stepwise logistic regression analysis was conducted. Furthermore, bioinformatics analysis was performed on this selected miRNA panel. Results: In ccRCC patients, the serum expression level of miRNA-142-5p was found to be significantly elevated compared to healthy controls (HCs), whereas the expression levels of let-7f-5p, miRNA-27b-3p, miRNA-212-3p, and miRNA-216-5p were significantly reduced. To assess their diagnostic potential for ccRCC, receiver operating characteristic (ROC) curve analysis was performed. The analysis revealed that miRNA-27b-3p, let-7f-5p, and miRNA-142-5p exhibited moderate diagnostic capabilities for ccRCC, with area under the curve (AUC) values of 0.826, 0.828, and 0.643, respectively. To further enhance diagnostic accuracy, a final diagnostic panel consisting of these three miRNAs was constructed, demonstrating good diagnostic value with an AUC of 0.952. Conclusions: The miRNA serum biomarker panel (miRNA-27b-3p, let-7f-5p, and miRNA-142-5p) identified in this study holds promise for early, non-invasive, and accurate diagnosis of ccRCC. This panel could potentially provide a valuable tool in clinical settings to aid in the timely detection and management of ccRCC.
The Effects of Chiropractic Spinal Adjustment on EEG in Adults with Alzheimer's and Parkinson's Disease: A Pilot Randomised Cross-over Trial
Authors: Navid Muhammad Samran , Niazi Imran Khan , Holt Kelly , Nedergaard Rasmus Bach , Amjad Imran , Ghani Usman , Kumari Nitika , Shafique Muhammad , Duehr Jenna , Trager Robert J. , Haavik Heidi
Published: May 2024

Objectives: In this study, we explored the effects of chiropractic spinal adjustments on resting-state electroencephalography (EEG) recordings and early somatosensory evoked potentials (SEPs) in Alzheimer’s and Parkinson’s disease. Methods: In this randomized cross-over study, 14 adults with Alzheimer’s disease (average age 67 ± 6 years, 2 females:12 males) and 14 adults with Parkinson’s disease (average age 62 ± 11 years, 1 female:13 males) participated. The participants underwent chiropractic spinal adjustments and a control (sham) intervention in a randomized order, with a minimum of one week between each intervention. EEG was recorded before and after each intervention, both during rest and stimulation of the right median nerve. The power-spectra was calculated for resting-state EEG, and the amplitude of the N30 peak was assessed for the SEPs. The source localization was performed on the power-spectra of resting-state EEG and the N30 SEP peak. Results: Chiropractic spinal adjustment significantly reduced the N30 peak in individuals with Alzheimer’s by 15% (p = 0.027). While other outcomes did not reach significance, resting-state EEG showed an increase in absolute power in all frequency bands after chiropractic spinal adjustments in individuals with Alzheimer’s and Parkinson’s disease. The findings revealed a notable enhancement in connectivity within the Default Mode Network (DMN) at the alpha, beta, and theta frequency bands among individuals undergoing chiropractic adjustments. Conclusions: We found that it is feasible to record EEG/SEP in individuals with Alzheimer’s and Parkinson’s disease. Additionally, a single session of chiropractic spinal adjustment reduced the somatosensory evoked N30 potential and enhancement in connectivity within the DMN at the alpha, beta, and theta frequency bands in individuals with Alzheimer’s disease. Future studies may require a larger sample size to estimate the effects of chiropractic spinal adjustment on brain activity. Given the preliminary nature of our findings, caution is warranted when considering the clinical implications. Clinical Trial Registration: The study was registered by the Australian New Zealand Clinical Trials Registry (registration number ACTRN12618001217291 and 12618001218280).
Disrupted Functional Brain Network Architecture in Sufferers with Boxing-Related Repeated Mild Traumatic Brain Injury: A Resting-State EEG Study
Authors: Wu Shukai , Fu Zhenghao , Wang Shuochen , Zheng Feng , Qiu Weizhi , Xu Guozheng , Zhang Dankui , Song Jian
Published: May 2024

Background: Repetitive mild traumatic brain injury (rmTBI) often occurs in individuals engaged in contact sports, particularly boxing. This study aimed to elucidate the effects of rmTBI on phase-locking value (PLV)-based graph theory and functional network architecture in individuals with boxing-related injuries in five frequency bands by employing resting-state electroencephalography (EEG). Methods: Twenty-fore professional boxers and 25 matched healthy controls were recruited to perform a resting-state task, and their noninvasive scalp EEG data were collected simultaneously. Based on the construction of PLV matrices for boxers and controls, phase synchronization and graph-theoretic characteristics were identified in each frequency band. The significance of the calculated functional brain networks between the two populations was analyzed using a network-based statistical (NBS) approach. Results: Compared to controls, boxers exhibited an increasing trend in PLV synchronization and notable differences in the distribution of functional centers, especially in the gamma frequency band. Additionally, attenuated nodal network parameters and decreased small-world measures were observed in the theta, beta, and gamma bands, suggesting that the functional network efficiency and small-world characteristics were significantly weakened in boxers. NBS analysis revealed that boxers exhibited a significant increase in network connectivity strength compared to controls in the theta, beta, and gamma frequency bands. The functional connectivity of the significance subnetworks exhibited an asymmetric distribution between the bilateral hemispheres, indicating that the optimized organization of information integration and segregation for the resting-state networks was imbalanced and disarranged for boxers. Conclusions: This is the first study to investigate the underlying deficits in PLV-based graph-theoretic characteristics and NBS-based functional networks in patients with rmTBI from the perspective of whole-brain resting-state EEG. Joint analyses of distinctive graph-theoretic representations and asymmetrically hyperconnected subnetworks in specific frequency bands may serve as an effective method to assess the underlying deficiencies in resting-state network processing in patients with sports-related rmTBI.
Nuclear Factor Erythroid 2-Related Factor 2 as a Potential Therapeutic Target in Neonatal Hypoxic-Ischemic Encephalopathy
Authors: Liu Jian-Xia , Zheng Da , Chen Ling , Chen Su , Min Jia-Wei
Published: May 2024

Hypoxic-ischemic encephalopathy (HIE) is a prominent cause of neonatal mortality and neurodevelopmental disorders; however, effective therapeutic interventions remain limited. During neonatal hypoxic-ischemic injury events, increased reactive oxygen species (ROS) production and decreased antioxidant levels lead to the induction of oxidative stress, which plays a pivotal role in the pathological process of neonatal HIE. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a key endogenous antioxidant transcription factor that protects against oxidative stress by promoting the transcription of various antioxidant genes. It has been demonstrated that Nrf2 signaling pathway activation by different compounds may protect against neonatal HIE. This review outlines the role of oxidative stress in neonatal HIE and summarizes the impact of antioxidants on neonatal HIE via activation of the Nrf2 signaling pathway. In conclusion, Nrf2 signaling pathway potentially exerts antioxidant, anti-inflammatory, antiapoptotic and antiferroptotic effects, thereby emerging as a focal point for future neonatal HIE treatment strategies.
Unconfined turbidity current interactions with oblique slopes: deflection, reflection and combined-flow behaviours
Authors: Wang Ru , Peakall Jeff , Hodgson David , Keavney Edward , Brown Helena , Keevil Gareth
Published: May 2024

What is the nature of flow reflection, deflection and combined-flow behaviour when gravity flows interact with slopes? In turn, how do these flow dynamics control sedimentation on slopes? Here, these questions are addressed using physical experiments, with low-density unconfined gravity flows interacting with slopes of varying gradients, at a range of flow incidence angles. The present paradigm for gravity current interaction with slopes was based on experiments with high-density flows, conducted in narrow 2D flume tanks, in small (1 m2 planform) 3D tanks, or in large 3D tanks where flows can surmount the topography. Here, larger-scale physical experiments were undertaken in unconfined settings where the flow cannot surmount a planar topographic slope. The experiments show that the dominant flow-process transitions from divergence-dominated, through reflection-dominated to deflection-dominated as the flow incidence angle varies from 90° to 15° and the slope gradient changes from 20° to 40°. Also, patterns of velocity pulsing at the base of, and on, the slope vary as a function of both the flow incidence angle and slope gradient. Furthermore, in all configurations complex multidirectional combined flows are observed on, or at the base of, the slope, and are shown to vary spatially across the slope. The findings challenge the paradigm of flow deflection and reflection in existing flow-topography process models that has stood for three decades. A new process model for flow-slope interactions is presented, that provides new mechanics for the frequent observation of palaeocurrents from sole marks at high angles to those in the associated ripple division. Results provide insights into the formation and spatial distribution of distinctive combined-flow bedforms, sediment dispersal patterns, and process controls on onlap termination styles in deep-sea settings, which can be applied to refine interpretations of exhumed successions.
The Consequences of Sexual Compliance Scale (CSCS): Scale Development and Psychometric Properties
Authors: Nickull Sabina , Lagerström Martin , Jern Patrick , Gunst Annika
Published: May 2024

Sexual compliance (i.e., to consent to sexual activity despite the lack of initial desire for it) is common in committed relationships. Previous research has shown that sexual compliance can have both positive and negative consequences for the well-being of the individual and the relationship. The aim of the present study was to develop a scale to measure the perceived consequences of sexual compliance, using exploratory factor analysis. The scale items were developed based on previous research and administered to a Finnish population-based sample of adults (N = 1,159). A two-factor model, explaining 40% of the total variance, was selected to create the Consequences of Sexual Compliance Scale (CSCS). Redundant items were excluded, and the final scale included two subscales with 10 items measuring perceived positive consequences (Cronbach’s α = .91) and 10 items measuring perceived negative consequences of sexual compliance (Cronbach’s α = .82). The individuals comprising the present sample experienced significantly more positive consequences compared to negative ones, with a large effect size (Cohen’s d = 2.2).
Error report for Wessel (2018)
Authors: ERROR
Published: May 2024

DECISION AND RECOMMENDATIONWessel (2018) “Prepotent motor activity and inhibitory control demands in different variants of the go/no-go paradigm” was determined to contain Minor Errors. That is, errors that have the benefit of being detectable thanks to the presence and sharing of research materials, but whose scope and implications on the conclusions of the article are minor. The detected errors do not rise to the level of recommending that a correction be issued. I thank Prof Wessel for nonetheless indicating his interest in pursuing such a correction. Authors can of course pursue a correction unilaterally if they choose to do so. Following the ERROR project’s emergent guidelines, the recommendations associated with a decision of ‘Minor Errors’ are as follows: - The report, author response, and recommendation have been posted on the ERROR website (error.reviews/reviews/wessel-2018) and as a preprint on PsyArXiv (osf.io/preprints/psyarxiv/8gtz2). Their associated materials have been posted to OSF (see osf.io/2q3ay).- The author is asked to recognise these errors in future discussions of the article.
In search of an integrative method to study unconscious processing: an application of Bayesian and General Recognition Theory models to the processing of hierarchical patterns in the absence of awareness.
Authors: Prieto Antonio , Montoro Pedro R. , Jimenez Mikel , Hinojosa José Antonio
Published: May 2024

The dissociation between conscious and unconscious perception is one of the most controversial issues in the study of consciousness. Some authors argue in favor of the existence of high-level unconscious processing, while others claim that most of the results obtained can be explained by a mixture of methodological issues and erroneous (or at least questionable) assumptions about what can be considered non-conscious. Particularly, one of the most debated topics involves the way in which the awareness of the stimuli is assessed, with a wide variety of measures and associated methods being proposed throughout the history of the field. To address this question, in the present study an integrative paradigm was devised to assess the extent to which masked hierarchical stimuli can be processed in the absence of awareness. We combined a primming task where participants were presented with Navon-like hierarchical masked stimuli, with the use of subjective and objective awareness measures collected either in a separate block (offline) or trial-by-trial during the main task (online). The unconscious processing of the masked primes was then evaluated through two different novel model-based methods. A Bayesian modeling approach and a General Recognition Theory modeling approach. Crucially, these two approaches assess unconscious processing through different theoretical assumptions and employing different awareness measures. The results showed that although there is a high correlation between awareness measures, the use of alternative approaches under different theoretical assumptions to analyze unconscious processing, leads to somewhat diverging conclusions about the extent of the unconscious processing of the masked primes.