bluuebunny/crossref_metadata_2025 huggingface dataset:
EDUKASI JAJANAN SEHAT SEBAGAI UPAYA PENCEGAHAN STUNTING PADA ANAK USIA PRASEKOLAH
Authors: Kelrey Fathimah , Mars Clara liem Glorya
Published: January 2025

Jajanan sehat merupakan bagian dari makanan aman yang bergizi yang memegang peranan penting bagi pertumbuhan dan mencegah terjadinya stunting. Angka kejadian stunting pada Balita termasuk anak usia prasekolah di Indonesia masih cukup tinggi sehingga perlu dicegah karena beresiko terhadap kurangnya pemenuhan nutrisi yang optimal. Untuk mengatasi hal tersebut diperlukan edukasi Kesehatan mengenai jajanan sehat. Tujuan dilakukan kegiatan ini diharapkan anak anak usia prasekolah mempunyai pemahaman tentang jajanan sehat sejak dini.  Metode edukasi yang digunakan dalam pengabdian masyarakat ini yaitu ceramah, diskusi, tanya jawab dan menggunakan media lembar balik. Hasil kegiatan ini menunjukkan adanya peningkatan pengetahuan dari peserta tentang jajanan sehat pada anak usia prasekolah di TK Angasa Lanud Pattimura Ambon dibuktikan dengan anak mampu memilih atau membedakan jajanan yang sehat dan jajanan yang tidak sehat. Kesimpulan dari kegiatan ini adalah edukasi jajanan sehat pada anak usia prasekolah melalui penyuluhan kesehatan dapat meningkatkan pengetahuan anak tentang jajanan sehat.
Exoplanet detection in rotational shearing interferometry through experimental setup and digital filtering techniques
Authors: Montes-Flores Manuel , Garcia-Torales Guillermo , Strojnik Marija
Published: January 2025

The significant brightness contrast between stars and orbiting planets often hinders the detection of exoplanets. This paper presents the development and validation of an experimental setup and digital filtering techniques for a rotational shearing interferometer (RSI) aimed at enhancing exoplanet detection. The method leverages controlled phase shifts and spatial frequency modulation through Risley and Dove prisms to isolate faint planetary signals from dominant starlight. Laboratory experiments use HeNe lasers to simulate a star-planet system, and spatial filters ensure precise wavefront alignment. The interferometer’s rotational shearing capabilities enhance the accuracy of phase alignment, allowing for significant suppression of starlight and improved detection of planetary signals. Additionally, applying Fourier-based digital filtering techniques further enhances detection sensitivity by reducing background noise. Experimental results demonstrate an 80% reduction in noise and up to a 20% increase in detection sensitivity compared to traditional interferometric methods. The RSI’s performance represents a significant advancement in interferometric techniques, suggesting its potential for real-world astronomical applications. However, further optimization is required to address challenges associated with space-based observations. This work sets the foundation for future research aimed at refining optical configurations and digital filtering techniques for exoplanet detection.
Loading rate, geometry, and damage state influence vertical extraction biomechanics in an ex vivo swine dental model
Authors: Gadzella Timothy J. , Rabey Karyne N. , Doschak Michael R. , Westover Lindsey , Addison Owen , Romanyk Dan L.
Published: January 2025

Introduction

Validated models describing the biomechanics of tooth extraction are scarce. This study seeks to perform experimental and numerical characterization of vertical tooth extraction biomechanics in swine incisors with imposed vertical extraction loads. Imaging analysis related mechanical outcomes to tooth geometry and applied loading rate. Then, the predictive capabilities of the developed finite element analysis (FEA) models were demonstrated by testing different loading scenarios and validating the results against experimental equivalents.

Methods

Simulated vertical extractions were performed on partial swine central incisors (n = 49) and studied for peak extraction force and dental complex stiffness. Post-extraction µCT images were obtained to measure root surface attachment area (RSAA) and observe patterns of periodontal ligament (PDL) rupture. Crosshead force-displacement data was used in an inverse finite element analysis (IFEA) to verify parameters for the PDL in an axisymmetric model of tooth extraction. New force-hold loading protocols were devised in silico and validated in a series of tests on swine incisors to demonstrate the predictive efficacy of the finite element model. Force-hold loading on an initially-damaged PDL was also simulated.

Results

Reductions in loading rate and RSAA were found to significantly reduce peak extraction forces by 98N–120 N. Increases in instantaneous stiffness during loading were associated with increases in loading rate. Inverse finite element solutions demonstrated consistent PDL parameters across loading cases. Force-hold loading predicted extraction behaviour with large variance in extraction time. Damage imposed in the FEA model was able to predict experimental results from experiments on similarly-damaged dental complexes.

Conclusion

This study presents a comprehensive experimental and numerical characterization of vertical tooth extraction biomechanics employing an ex vivo swine model. The results of these experiments suggest that the axisymmetric FEA model is a powerful tool for predicting a range of conditions and dental complex geometries. The predictive power of the FEA model demonstrated in this study encourages its use in pre-clinical testing and development of new vertical extraction loading schemes for improving clinical outcomes.
Biomimetic bioreactor for potentiated uricase replacement therapy in hyperuricemia and gout
Authors: Yang Bin , Luo Guihu , Nie Tailei , Ban Zhenglan , Ning Quanxin , Zhang Jialin , Liu Xiangru , Lin Yanhua , Xie Xiaochun , Chen Qianyun , Zhong Han , Huang Ying , Liao Pan , Liu Yan , Guo Chenyang , Cheng Chuanxu , Sun Erwei
Published: January 2025

Introduction

Uricase replacement therapy is a promising approach for managing hyperuricemia and gout but is hindered by challenges such as short blood circulation time, reduced catalytic activity, and excessive hydrogen peroxide (H2O2) production. These limitations necessitate innovative strategies to enhance therapeutic efficacy and safety.

Methods

We designed and synthesized RBC@SeMSN@Uri, a red blood cell-coated biomimetic self-cascade bioreactor, which encapsulates uricase (Uri) and a selenium-based nano-scavenger (SeMSN) within RBC membranes. This design aims to reduce immunogenicity, extend systemic circulation, and maintain enzymatic activity. In vitro assays were conducted to evaluate biocompatibility, anti-inflammatory effects, and oxidative stress protection. In vivo experiments in hyperuricemia and gout models assessed therapeutic efficacy, biodistribution, and biosafety.

Results

RBC@SeMSN@Uri effectively degraded uric acid (UA) into allantoin and converted H2O2 into water, preventing oxidative damage and inflammation. In vitro assays demonstrated excellent biocompatibility and reduced H2O2-induced inflammatory responses compared to free uricase. In vivo, the bioreactor prolonged circulation time, significantly reduced uric acid levels, alleviated kidney damage, and mitigated symptoms of hyperuricemia and gout. It also targeted inflamed joints, reducing swelling and inflammation in gouty arthritis models.

Discussion

This study presents RBC@SeMSN@Uri as a novel biomimetic strategy for enzyme replacement therapy in hyperuricemia and gout. By integrating uricase and selenium-based nano-scavenger within RBC membranes, the bioreactor addresses key limitations of traditional therapies, offering enhanced stability, reduced immunogenicity, and superior therapeutic efficacy. This platform holds potential for broader applications in protein or antibody delivery for enzyme replacement therapies in other diseases.
Relevance of the GH-VEGFB/VEGFA axis in liver grafts from brain-dead donors with alcohol-associated liver disease
Authors: Micó-Carnero Marc , Rojano-Alfonso Carlos , Maroto-Serrat Cristina , Cutrin Juan Carlos , Casillas-Ramírez Araní , Peralta Carmen
Published: January 2025

Introduction

Grafts with alcohol-associated liver disease (ALD) subjected to prolonged cold ischaemia from donors after brain death (DBD) are typically unsuitable for transplantation. Here, we investigated the role of growth hormone (GH) in livers with ALD from DBDs and its relationship with vascular endothelial growth factor A (VEGFA) and VEGFB.

Methods

Livers from rats fed ethanol for 6 weeks and with brain death (BD) were cold stored for 24 h and subjected to ex vivo reperfusion. Hepatic damage and proliferative and inflammatory parameters were analysed after BD, before graft retrieval, and after reperfusion. Survival was monitored using an in vivo transplantation model.

Results

In DBDs, the administration of GH, which increased the levels in the intestine but not in the liver, induced the generation of both VEGFA and VEGFB in the intestine and protected against hepatic damage caused by BD before retrieving liver grafts from donors. However, VEGFA was the only factor that protected against damage after cold ischemia and reperfusion, which also increased the survival of the recipients.

Discussion

In conclusion, the signalling pathway and beneficial properties of the GH-VEGFA/VEGFB pathway, in which the intestine-liver axis plays a key role, were disrupted when grafts with ALD from DBDs were retrieved from donors and subjected to cold ischemia and reperfusion.
Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues
Authors: Utkina Marina , Shcherbakova Anastasia , Deviatiiarov Ruslan , Ryabova Alina , Loguinova Marina , Trofimov Valentin , Kuznetsova Anna , Petropavlovskiy Mikhail , Salimkhanov Rustam , Maksimov Denis , Albert Eugene , Golubeva Alexandra , Asaad Walaa , Urusova Lilia , Bondarenko Ekaterina , Lapshina Anastasia , Shutova Alexandra , Beltsevich Dmitry , Gusev Oleg , Dzeranova Larisa , Melnichenko Galina , Minniakhmetov Ildar , Dedov Ivan , Mokrysheva Natalya , Popov Sergey
Published: January 2025

Current dissociation methods for solid tissues in scRNA-seq studies do not guarantee intact single-cell isolation, especially for sensitive and complex human endocrine tissues. Most studies rely on enzymatic dissociation of fresh samples or nuclei isolation from frozen samples. Dissociating whole intact cells from fresh-frozen samples, commonly collected by biobanks, remains a challenge. Here, we utilized the acetic-methanol dissociation approach (ACME) to capture transcriptional profiles of individual cells from fresh-frozen tissue samples. This method combines acetic acid-based dissociation and methanol-based fixation. In our study, we optimized this approach for human endocrine tissue samples for the first time. We incorporated a high-salt washing buffer instead of the standard PBS to stabilize RNA and prevent RNases reactivation during rehydration. We have designated this optimized protocol as ACME HS (ACetic acid-MEthanol High Salt). This technique aims to preserve cell morphology and RNA integrity, minimizing transcriptome changes and providing a more accurate representation of mature mRNA. We compared the ability of enzymatic, ACME HS, and nuclei isolation methods to preserve major cell types, gene expression, and standard quality parameters across 41 tissue samples. Our results demonstrated that ACME HS effectively dissociates and fixes cells, preserving cell morphology and high RNA integrity. This makes ACME HS a valuable alternative for scRNA-seq protocols involving challenging tissues where obtaining a live cell suspension is difficult or disruptive.
The role of Golgi complex proteins in cell division and consequences of their dysregulation
Authors: Iannitti Roberta , Mascanzoni Fabiola , Colanzi Antonino , Spano Daniela
Published: January 2025

The GC (Golgi complex) plays a pivotal role in the trafficking and sorting of proteins and lipids until they reach their final destination. Additionally, the GC acts as a signalling hub to regulate a multitude of cellular processes, including cell polarity, motility, apoptosis, DNA repair and cell division. In light of these crucial roles, the GC has garnered increasing attention, particularly given the evidence that a dysregulation of GC-regulated signalling pathways may contribute to the onset of various pathological conditions. This review examines the functions of the GC and GC-localised proteins in regulating cell cycle progression, in both mitosis and meiosis. It reviews the involvement of GC-resident proteins in the formation and orientation of the spindle during cell division. In light of the roles played by the GC in controlling cell division, this review also addresses the involvement of the GC in cancer development. Furthermore, TCGA (The Cancer Genome Atlas) database has been queried in order to retrieve information on the genetic alterations and the correlation between the expression of GC-localised proteins and the survival of cancer patients. The data presented in this review highlight the relevance of the GC in regulating cell cycle progression, cellular differentiation and tumourigenesis.
Clinical features and risk factors of HIV-infected patients with intracerebral hemorrhage: a retrospective study with propensity score matching analysis
Authors: Huang Qiuhui , Chen Shengri , Huang Hua , Deng Xuhui , Cen Gengyu , Wang Miao , Liang Zhijian
Published: January 2025

Purpose

To investigate the clinical features and risk factors of the human immunodeficiency virus (HIV)-infected patients with intracerebral hemorrhage (ICH).

Patients and methods

The patients with HIV-infected without ICH group were matched to the group of HIV-infected ICH patients. Logistic regression analysis using 1:1 propensity score matching (PSM) was performed to investigate the independent risk factors for ICH in HIV-infected patients. The receiver operating characteristic (ROC) curve was configured to calculate the optimal predictors of ICH in HIV-infected patients.

Results

A total of 59 HIV-infected patients with ICH and 180 HIV-infected patients without ICH were included. A cohort of 118 patients was ascertained utilizing PSM. Multivariate binary logistic regression analysis revealed that drug abuse-related HIV-infected, prolonged prothrombin time (PT), and elevated triglyceride (TG) levels were independent risk factors of ICH in HIV-infected patients. The ROC curve demonstrated that the combined predictor, composed of drug abuse-related HIV-infected, prolonged PT, and elevated TG levels, exhibited the highest area under the curve (AUC), with a cut-off point at 0.426, sensitivity of 78%, and specificity of 81.4%.

Conclusion

The present study revealed that a valuable factor combined with drug abuse-related HIV-infected, prolonged PT, and elevated serum TG levels could serve as predictors of ICH in HIV-infected patients.
Plasma Epstein-Barr Virus DNA load for diagnostic and prognostic assessment in intestinal Epstein-Barr Virus infection
Authors: Ma Chunxiang , Jiang Mingshan , Li Jiaxin , Zeng Zhen , Wu Yushan , Cheng Rui , Lin Hao , Pang Jiangmei , Yin Fang , Jia Yongbin , Li Lili , Zhang Hu
Published: January 2025

Background

The prospective application of plasma Epstein-Barr virus (EBV) DNA load as a noninvasive measure of intestinal EBV infection remains unexplored. This study aims to identify ideal threshold levels for plasma EBV DNA loads in the diagnosis and outcome prediction of intestinal EBV infection, particularly in cases of primary intestinal lymphoproliferative diseases and inflammatory bowel disease (IBD).

Methods

Receiver operating characteristic (ROC) curves were examined to determine suitable thresholds for plasma EBV DNA load in diagnosing intestinal EBV infection and predicting its prognosis.

Results

108 patients were retrospectively assigned to the test group, while 56 patients were included in the validation group. Plasma EBV DNA loads were significantly higher in the intestinal EBV infection group compared to the non-intestinal EBV infection group (Median: 2.02 × 102 copies/mL, interquartile range [IQR]: 5.49 × 101-6.34×103 copies/mL versus 4.2×101 copies/mL, IQR: 1.07 ×101-6.08×101 copies/mL; P < 0.0001). Plasma EBV DNA levels at 9.21×101 and 6.77×101 copies/mL proved beneficial for the identification and prognostication in intestinal EBV infection, respectively. Values of 0.82 and 0.71 were yielded by the area under the ROC curve (AUC) in the test cohort, corresponding to sensitivities of 84.38% (95% confidence interval [95%CI]: 68.25%-93.14%) and 87.5% (95%CI: 69%-95.66%), specificities of 83.33% (95%CI: 64.15%-93.32%) and 68.09% (95%CI: 53.83%-79.6%), positive predictive values (PPV) of 87.1% (95%CI: 71.15%-94.87%) and 58.33% (95%CI: 42.2%-72.86%), and positive likelihood ratios (LR+) of 5.06 and 2.74 in the validation cohort, respectively. Furthermore, a plasma EBV DNA load of 5.4×102 copies/mL helped differentiate IBD with intestinal EBV infection from primary intestinal EBV-positive lymphoproliferative disorders (PIEBV+LPDs), achieving an AUC of 0.85 within the test cohort, as well as 85% sensitivity (95%CI: 63.96%-94.76%), 91.67% specificity (95%CI: 64.61%-99.57%), 94.44% PPV (95%CI: 74.24%-99.72%), and an LR+ of 10.2 in the validation cohort.

Conclusions

Plasma EBV DNA load demonstrates notable potential in distinguishing between different patient cohorts with intestinal EBV infection, although its sensitivity requires further optimization for clinical application.
Prognostic impact of coronary microvascular dysfunction assessed by AMR in acute coronary syndrome patients with chronic kidney disease
Authors: Guo Ziyu , Li Yike , Chen Qiang , Zheng Jingang
Published: January 2025

Background

Angiography-derived microcirculatory resistance (AMR) is proposed as a novel, pressure- temperature-wire-free and less-invasive method to evaluate coronary microvascular dysfunction (CMD). This study aims to examine the prognostic role of CMD assessed by AMR in predicting adverse events in acute coronary syndrome (ACS) patients with chronic kidney disease (CKD).

Methods

This retrospective cohort study included ACS with CKD patients in the China-Japan Friendship Hospital from January 2016 to November 2022. The patients were divided into CMD and non-CMD groups based on AMR values of less than or greater than 250 mmHg*s/m.

Results

A total of 345 eligible patients were included in this study. During a median follow-up of 23.0 months, higher prevalence rate of MACEs (28.3% vs. 15.1%, P = 0.003) and death (20.2% vs. 4.1%, P = 0.001) were observed in the CMD group. In multivariate Cox regression analysis, patients in the group of CMD had a 1.843 times higher hazard ratio (HR) for developing MACEs (HR: 1.843, 95% CI: 1.071–3.174, P = 0.027) and 5.325 times higher HR for developing death (HR: 5.325, 95% CI: 1.979–14.327, P < 0.001) for every 10 mmHg*s/m increment in AMR. The incorporation of AMR improved the predictive accuracy of the GRACE score for MACEs and death.

Conclusion

This study indicates that the AMR is significantly related to poor prognosis among patients with ACS and CKD. Furthermore, AMR could improve the predictive power of the GRACE risk score. These results indicated that AMR may serve as a valuable clinical tool for classification, risk stratification or therapy individualization in these patients.
Constructing a metadata knowledge graph as an atlas for demystifying AI pipeline optimization
Authors: Venkataramanan Revathy , Tripathy Aalap , Kumar Tarun , Serebryakov Sergey , Justine Annmary , Shah Arpit , Bhattacharya Suparna , Foltin Martin , Faraboschi Paolo , Roy Kaushik , Sheth Amit
Published: January 2025

The emergence of advanced artificial intelligence (AI) models has driven the development of frameworks and approaches that focus on automating model training and hyperparameter tuning of end-to-end AI pipelines. However, other crucial stages of these pipelines such as dataset selection, feature engineering, and model optimization for deployment have received less attention. Improving efficiency of end-to-end AI pipelines requires metadata of past executions of AI pipelines and all their stages. Regenerating metadata history by re-executing existing AI pipelines is computationally challenging and impractical. To address this issue, we propose to source AI pipeline metadata from open-source platforms such as Papers-with-Code, OpenML, and Hugging Face. However, integrating and unifying the varying terminologies and data formats from these diverse sources is a challenge. In this study, we present a solution by introducing Common Metadata Ontology (CMO) which is used to construct an extensive AI Pipeline Metadata Knowledge Graph (AIMKG) consisting of 1.6 million pipelines. Through semantic enhancements, the pipeline metadata in AIMKG is also enriched for downstream tasks such as search and recommendation of AI pipelines. We perform quantitative and qualitative evaluations on AIMKG to search and recommend relevant pipelines to user query. For quantitative evaluation, we propose a custom aggregation model that outperforms other baselines by achieving a retrieval accuracy (R@1) of 76.3%. Our qualitative analysis shows that AIMKG-based recommender retrieved relevant pipelines in 78% of test cases compared to the state-of-the-art MLSchema-based recommender which retrieved relevant responses in 51% of the cases. AIMKG serves as an atlas for navigating the evolving AI landscape, providing practitioners with a comprehensive factsheet for their applications. It guides AI pipeline optimization, offers insights and recommendations for improving AI pipelines, and serves as a foundation for data mining and analysis of evolving AI workflows.
A review of NEST models for liquid xenon and an exhaustive comparison with other approaches
Authors: Szydagis M. , Balajthy J. , Block G. A. , Brodsky J. P. , Brown E. , Cutter J. E. , Farrell S. J. , Huang J. , Kamaha A. C. , Kozlova E. S. , Liebenthal C. S. , McKinsey D. N. , McMichael K. , McMonigle R. , Mooney M. , Mueller J. , Ni K. , Rischbieter G. R. C. , Trengove K. , Tripathi M. , Tunnell C. D. , Velan V. , Westerdale S. , Wyman M. D. , Zhao Z. , Zhong M.
Published: January 2025

This paper discusses the microphysical simulation of interactions in liquid xenon, the active detector medium in many leading rare-event searches for new physics, and describes experimental observables useful for understanding detector performance. The scintillation and ionization yield distributions for signal and background are presented using the Noble Element Simulation Technique (NEST), a toolkit based on experimental data and simple empirical formulas, which mimic previous microphysics modeling but are guided by data. The NEST models for light and charge production as a function of the particle type, energy, and electric field are reviewed, along with models for energy resolution and final pulse areas. NEST is compared with other models or sets of models and validated against real data, with several specific examples drawn from XENON, ZEPLIN, LUX, LZ, PandaX, and table-top experiments used for calibrations.
Construction of a scale measuring primary teachers’ self-efficacy to teach STEAM: the STEAM-TSES
Authors: Stepanović Ilić Ivana , Peixoto Francisco , Dabić Boričić Milana , Videnović Marina , Castro Silva Jose , Ferreira Nadia , Monteiro Vera
Published: January 2025

The STEAM-TSES was developed to measure teachers’ self-efficacy (TSE) in teaching STEAM disciplines due to the lack of instruments measuring specific TSE dimensions, especially in STEAM education. This paper presents the validation results based on a sample of 805 Portuguese and Serbian primary school teachers. The scale comprises 36 items measuring TSE related to mathematics, biology, chemistry, science, and arts, the ability to motivate students, and confidence in integrating STEAM disciplines. The measurement model tested through Exploratory Structural Equation Modeling and Confirmatory Factor Analysis showed an acceptable fit to the data, supporting the existence of nine factors: five related to TSE in teaching specific subjects, three focused on motivating students in mathematics, science, and arts, and one related to integrated teaching. The analyses show good psychometric characteristics of the STEAM-TSES, and the correlations with the TSE scale indicate the validity of distinguishing constructs associated with STEAM education.
Identification and analysis of pancreatic intraepithelial neoplasia: opportunities and challenges
Authors: Pian Ling-ling , Song Mei-hui , Wang Teng-fei , Qi Ling , Peng Tie-li , Xie Ke-ping
Published: January 2025

Pancreatic intraepithelial neoplasia (PanIN) is the most common precursor lesion of pancreatic ductal adenocarcinoma (PDAC), which has poor prognosis with a short median overall survival of 6-12 months and a low 5-year survival rate of approximately 3%. It is crucial to remove PanIN lesions to prevent the development of invasive PDAC, as PDAC spreads rapidly outside the pancreas. This review aims to provide the latest knowledge on PanIN risk, pathology, cellular origin, genetic susceptibility, and diagnosis, while identifying research gaps that require further investigation in this understudied area of precancerous lesions. PanINs are classified into PanIN 1, PanIN 2, and PanIN 3, with PanIN 3 having the highest likelihood of developing into invasive PDAC. Differentiating between PanIN 2 and PanIN 3 is clinically significant. Genetic alterations found in PDAC are also present in PanIN and increase with the grade of PanIN. Imaging methods alone are insufficient for distinguishing PanIN, necessitating the use of genetic and molecular tests for identification. In addition, metabolomics technologies and miRNAs are playing an increasingly important role in the field of cancer diagnosis, offering more possibilities for efficient identification of PanIN. Although detecting and stratifying the risk of PanIN poses challenges, the combined utilization of imaging, genetics, and metabolomics holds promise for improving patient survival in this field.
Increased risk of type 2 diabetes after traumatic amputation: a nationwide retrospective cohort study
Authors: Yoo Jung Eun , Lee Dagyeong , Kim Bongseong , Chang Won Hyuk , Jin Sang-Man , Han Kyungdo , Shin Dong Wook
Published: January 2025

Background

Amputation confers disabilities upon patients and is linked to cardiometabolic morbidity and mortality. We aimed to compare the incidence of type 2 diabetes (T2DM) between individuals following amputation with those of the general population.

Methods

We performed a population-based retrospective cohort study using the Nationwide Health Insurance Service database. A total of 21,343 individuals with amputation during 2010–2018 and their 1:3 age- and sex-matched controls was included. We conducted Cox proportional hazard analysis to calculate the risk of T2DM among individuals with amputation.

Results

During the 4.2 ± 2.5 year mean follow-up period, there were 912 incident T2DM cases (10.7 per 1,000 person-years) among individuals with amputation. Individuals with amputation had a higher risk for T2DM (adjusted hazard ratio [aHR] 1.11, 95% confidence interval [CI] 1.03–1.20) compared with matched controls. The risks were increased further when accompanied with disability; those with severe disability had a higher risk of T2DM (aHR 1.77, 95% CI 1.20–2.60) than matched controls. Individuals with proximal upper limb amputation (aHR 1.10, 95% CI 1.02–1.18) and proximal lower limb amputation (aHR 3.60, 95% CI 1.50–8.64) had a higher risk of T2DM compared with matched controls.

Conclusions

Individuals with amputation were at significantly greater risk for T2DM than the general population, particularly those with severe disability and proximal amputation. Innovative strategies that improve and support the long-term T2DM risk for severely injured individuals with proximal amputation are warranted.
The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency
Authors: Wang Wei , Shu Mingming , Li Jianhua , Wang Qihang , Zhang Wendan , Wang Ye , Guo Yiming , Cheng Yanbin , Jiang Honghong , Song Chunlan , Liu Yuan , Shang Wei
Published: January 2025

Introduction

Premature ovarian insufficiency (POI) is a condition characterized by ovarian dysfunction occurring before the age of 40, and its etiology is multifactorial, including genetic, immunological, infectious, environmental, and iatrogenic factors, with over half of the cases remaining unexplained. Whether the microbial communities and metabolites in follicular fluid, which is the direct microenvironment for oocyte survival, are related to POI has not been reported.

Methods

In this study, Follicular fluid samples of 26 patients with POI and 27 controls with a normal ovarian reserve were collected and analyzed using 16S rDNA sequencing and untargeted metabolomics. Conjoint analysis was performed to identify key microbial communities and metabolites that might be involved in POI.

Results

Patients with POI exhibited significant alterations in microbial richness and diversity and metabolic profile in their follicular fluid. The downregulation of ABC transporters and upregulation of the citrate cycle (TCA cycle) might be critical for the development and progression of POI. G-Rhodopseudomonas and g-Caulobacter were identified as key microbial genera, while L-aspartic acid, citrate, isoleucine, and cytidine were identified as key metabolites.

Discussion

These findings offer novel insights into the pathogenesis of POI and might pave the way for improved clinical outcomes for individuals with POI.
Family planning and preimplantation testing: family experiences in congenital adrenal hyperplasia
Authors: Sandy Jessica L. , Betts Grant , Harper Jessica L. , Nevin Suzanne M. , Deans Rebecca , Neville Kristen A.
Published: January 2025

Introduction

Pre-implantation testing (PGT) is often suggested by healthcare professionals (HCP) to parents of children with congenital adrenal hyperplasia (CAH) considering subsequent children. Despite this, some families choose to conceive naturally without genetic testing and intervention. The aims of this study were to explore fertility choices of couples with a child with CAH and the decision making process and perceptions behind these choices, and to explore the families’ lived experiences with CAH and the couples’ subsequent fertility journey. A better healthcare professional understanding of these experiences may subsequently help guide clinicians to better manage and support families of children with CAH and other autosomal recessive conditions.

Methods

All parents of current children of a tertiary service in 2020 with 21-hydroxylase deficient CAH who made an active decision regarding family planning after diagnosis of their index child were invited to participate in a semi-structured interview. Thematic analysis was performed using an inductive, semantic approach.

Results

Thirty families (34 children) were identified. Fourteen considered subsequent children and had directed genetic counselling. Eight decided to have additional children of whom seven agreed to participate. Thematic analysis identified six key domains. Psychological impact surrounding the CAH diagnosis was long-lasting, causing symptoms of trauma including depression and anxiety, and influencing a couple’s choice to pursue PGT to avoid having another affected child. The perception of the index child having a mild phenotype, and fear of a more severe phenotype, often supported this decision. Conversely, lived experience of CAH and low day-to-day impact, along with a negative experience of PGT, with a greater than anticipated financial, physical, and emotional toll, led some families to subsequently consider natural conception. The role of the healthcare professional (HCP) was important in the CAH and family planning journeys. A perceived poor understanding of CAH, overstating its potential seriousness, contributed to distress. Parents reported feeling pressured to undergo PGT. Peer-support had a universally positive impact on family experience.

Discussion/conclusions

This study highlights the complex and dynamic nature of fertility decision-making, and the importance of HCP empathy and open-mindedness. Education of HCP and encouraging peer support may improve the CAH and fertility journey for families.
Case report: Prolonged and severe hungry bone syndrome after parathyroidectomy in X-linked hypophosphatemia
Authors: Puliani Giulia , Hasenmajer Valeria , Spaziani Matteo , Frusone Federico , Tarantino Chiara , Angelini Francesco , Vincenzi Ludovica , Lubrano Riccardo , Marcellino Alessia , Biffoni Marco , Isidori Andrea M.
Published: January 2025

Tertiary hyperparathyroidism is characterized by hypercalcemia resulting from autonomous parathyroid hormone production and usually occurs after a prolonged period of secondary hyperparathyroidism. This condition can be a complication of X-linked hypophosphatemia (XLH), a rare genetic disease characterized by renal phosphate loss and consequent hypophosphatemia. Parathyroidectomy is considered the first-line therapy but surgical intervention can be complicated by hungry bone syndrome. A male Caucasian patient presented with XLH, diagnosed at the age of 3 years. At the age of 21, tertiary hyperparathyroidism occurred. Neck ultrasonography, neck magnetic resonance imaging, and 99Tc-sestamibi parathyroid scintigraphy revealed two hyperplastic parathyroid glands. To minimize the risk of hypercalcemia, calcimimetic therapy was initiated. After 6 months and preparation with 1,25-dihydroxy vitamin D, the patient underwent total parathyroidectomy with autotransplantation of half of a parathyroid gland into the sternocleidomastoid muscle. Histopathological examination revealed diffuse microscopical hyperplasia of the parathyroid glands. Despite oral supplementation with calcium carbonate and calcitriol, severe hypocalcemia developed on the second postoperative day, attributable to hungry bone syndrome. This finding was confirmed by an increase in bone turnover markers and a reduction in urinary calcium excretion. Hypocalcemia correction required continuous infusion of calcium gluconate for over 2 months. After approval, the patient began burosumab therapy with significant benefits. This case illustrates the complexity of treating tertiary hyperparathyroidism and mineral metabolism in patients with XLH. The hungry bone syndrome can complicate parathyroidectomy, exposing the patients to life-threatening risks. Burosumab therapy may reduce the risk of tertiary hyperparathyroidism developing in these patients.
Investigating the asymmetric impact of tourism, green fiscal policy, and fintech on environmental emissions and coastal water quality: an empirical study using the method of moments quantile regression
Authors: Yang Mengqi , Tang Xing
Published: January 2025

Introduction

The factors that significantly and negatively impact carbon dioxide (CO2) emissions and coastal water quality (CWQ) must be continuously monitored and thoroughly evaluated. Among these, tourism (TR) volume stands out as one of the primary contributors to such effects. In contrast, green fiscal policy (GFP) and fintech (FT) can be considered proactive and modern efforts contributing to the improvement of these environmental indicators. Exploring whether the impacts of these factors exhibit uniformity across quantiles will greatly benefit strategic solutions aimed at avoiding resource waste.

Methods

This paper aims to calibrate procedures to apply the method of moment quantile regression (MMQR) model to address this issue. Firstly, cross-sectional dependence (CSD) among the variables is examined. Next, a stable long-term relationship between the variables is assessed using stationarity analysis. Finally, the MMQR estimation is conducted to thoroughly investigate the impact of independent variables on CWQ and CO2 across different quantiles.

Results

The results from both the fixed effects (FE-OLS) and dynamic ordinary least squares (D-OLS) models reveal stable and significant correlations between the regressors and response variables. The research findings indicate that GFP and FT exert a significant impact on improving both CWQ and reducing CO2. In contrast, the favorable growth of the TR sector contributes negatively to CWQ and CO2.

Discussion

The paper recommends that the government increase spending and investment in green projects utilizing renewable energy, green transportation, blockchain technology, and advanced techniques. It also advocates for a strategic approach to controlling TR, focusing on enhanced waste management, in order to improve CWQ and CO2 indicators across most quantiles.
Serum proteomic and metabolomic profiling of hepatocellular carcinoma patients co-infected with Clonorchis sinensis
Authors: Tang Zeli , Wei Caibiao , Deng Xueling , Lin Qiumei , Hu Qiping , Li Shitao , Wang Jilong , Wu Yuhong , Liu Dengyu , Fang Min , Zhan Tingzheng
Published: January 2025

Background

Clonorchis sinensis (C. sinensis) infection is a significant risk factor for hepatocellular carcinoma (HCC), yet its underlying mechanisms remain poorly understood. This study aimed to investigate the impact of C. sinensis infection on the serum proteomic and metabolomic profiling of HCC patients, focusing on the potential mechanisms.

Method

A retrospective clinical analysis was conducted on 1121 HCC patients, comparing those with and without C. sinensis infection. The influence of C. sinensis on serum proteome and metabolome in HCC was further assessed.

Result

C. sinensis infection correlated with a younger age at cancer onset, male predominance, advanced cancer stage, liver cirrhosis, and microvascular invasion in HCC patients. It also associated with shorter overall survival (OS) and recurrence-free survival (RFS). The levels of blood lipids (e.g., APO-A, HDL-C, and TG) were significantly altered after C. sinensis infection. Proteomic and metabolomic analyses revealed metabolic reprogramming caused by C. sinensis, with excessive depletion of argininosuccinate synthase (ASS) and D-glucose as potential factors in C. sinensis-associated HCC malignancy. Key molecules ILF2, CNN2, OLFM4, NOTCH3, and LysoPA were implicated in HCC progression. Furthermore, C. sinensis triggered inflammation, insulin resistance, and pro-tumor immune escape, and exacerbated the complication of degenerative diseases.

Conclusion

This study not only provides compelling evidence for elucidating the mechanisms underlying C. sinensis-mediated HCC development but also identifies potential therapeutic targets for HCC patients co-infected with C. sinensis.
Murine model of cross-IgE sensitization and cross-anaphylactic reactions among multiple group food allergens
Authors: Musa Ibrahim , Ardalani Fariba , Yang Nan , Maleki Soheila J. , Li Xiu-Min
Published: January 2025

Rationale

Approximately 32 million people in the United States suffer from food allergies. Some food groups, such as legumes – peanuts, tree nuts, fish, and shellfish, have a high risk of cross-reactivity. However, the murine model of multiple food group cross-reactivity is limited.

Objective

We sought to develop a murine model that can be used to investigate novel therapeutics for the treatment of multiple food allergies.

Methods

C3H/HeJ mice were sensitized intraperitoneally (i.p.) once a week for three weeks with a mixture of 500µg of protein from peanut, cashew, walnut, shrimp, cod, and 2 mg Alum. The control group consisted of naïve mice. IgE levels against the sensitized allergens and their cross-reactive allergens were measured by ELISA at baseline and 3 weeks after sensitization. In weeks 4 and 5, the mice were given intragastric challenges with 200mg/mouse of each food: peanut, chickpea, lentil, cashew, almond, pistachio, hazelnut, brazil nut, walnut, pecan, shrimp, lobster, cod, salmon, and mackerel. After each challenge, anaphylactic symptoms, rectal temperatures, and plasma histamine were measured.

Results

There was a significant elevation of IgE against sensitized antigens (peanut cashew, walnut, shrimp, and cod) as well as cross-reactive allergens used for oral food challenge from legumes including peanut, chickpea, and lentil, as well as tree nuts such as cashew, almond, pistachio, hazelnut, brazil nut, walnut, and pecan. Furthermore, there was a significant increase in crustaceans such as shrimp, lobster and fish like cod, salmon, and mackerel (p<0.01). Consistently, significantly increased anaphylactic symptom scores (p<0.05), decreased rectal temperature (p<0.001), and increased plasma histamine (p<0.05) compared to the naïve mice occurred following each challenge with sensitized foods and unsensitized, but cross-reactive foods.

Conclusion

We generated a comprehensive murine model of IgE-mediated multiple food groups of cross-reactive anaphylaxes. This will provide an essential tool for developing novel therapies for cross-reactivity multiple food allergies.
Case report: Reversible splenial lesion syndrome preceding the onset of multiple sclerosis
Authors: Mauritz Matthias , Kliushnikova Dariia , Otto Ferdinand , Harrer Andrea , Moser Tobias , Radlberger Richard Friedrich , Kleindienst Waltraud , Trinka Eugen , Wipfler Peter
Published: January 2025

Background

The reversible splenial lesion syndrome is frequently associated with systemic and central nervous system infections. Whether an infection associated with the occurrence of the reversible splenial lesion syndrome could play a role in the later development of multiple sclerosis is unknown.

Methods

Case Report.

Results

A 27-year-old woman developed an infection-related reversible splenial lesion syndrome. Diagnostic findings did not establish a specific type of infection, but revealed evidence for a potential disposition towards autoimmunity. 32 months after the initial presentation, new clinical and radiological manifestations developed that led to a diagnosis of multiple sclerosis.

Conclusions

In susceptible individuals, infectious disease processes involving the central nervous system, such as described in this case, might be a factor in the pathogenesis of multiple sclerosis. More research on the prodromal stage of multiple sclerosis is needed to better understand the relationship between infections and autoimmunity.
Element contents changes during the propagule development of two Sonneratia species
Authors: Liu Chao , Zhang Lin , Shi Xiaofang , Tang Yanna , Wang Mao , Wang Wenqing
Published: January 2025

Vivipary is common in several mangrove species and is generally considered an adaptation to the intertidal saline environment. However, the coexistence of many nonviviparous mangroves makes this view controversial. This study investigated the propagule development of two nonviviparous mangrove species, Sonneratia alba and S. caseolaris, with marked differences in salt tolerance and distribution. Changes in the density, water content, and concentrations of the five main osmoregulatory elements (Cl, Na, K, Ca, and Mg) were determined. As the propagules of S. alba and S. caseolaris mature, the element concentrations (mg/g) in the propagules gradually decrease, indicating a desalination process. Moreover, the Cl, Na, Ca, and Mg content in the propagules were lower than in the mature leaves and calyx. Similar to viviparous mangroves, the development of the propagules of nonviviparous mangroves is also a desalination process. Although both viviparous and nonviviparous mangrove species undergo a desalination process during propagule development, our findings suggest that viviparity may not be solely defined by desalination, but rather by the extended period of low-salinity protection during early development on the maternal tree, which represents a key adaptation for survival in high-salinity environments. In contrast, nonviviparous mangroves, which rely on seed germination and early development in saline seawater, face additional challenges in high-salinity habitats, highlighting their distinct adaptive strategies.
Efficacy of traditional Chinese medicine Cordyceps sinensis as an adjunctive treatment in patients with renal dysfunction: a systematic-review and meta-analysis
Authors: Wu Fenfang , Xu Chunhua , Si Xinlei , He Fei , Xu Kang , Zhang Yu , Lin Shan
Published: January 2025

Objective

The effectiveness of using Cordyceps sinensis as an adjuvant therapy for patients with renal dysfunction (RD), especially acute kidney injury (AKI), is still a topic of debate. In response to the current conflicting data, the present meta-analysis was conducted to assess the clinical effectiveness of C. sinensis in the treatment of RD and to provide evidence for clinical practice.

Methods

Several databases, including PubMed, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI) and Wanfang, were systematically searched updated to March 25, 2024. We used the combined ratio (OR) and diagnostic ratio (DOR) to assess the therapeutic effect of C. sinensis. In addition, risk of bias was assessed by Cochrane Risk of Bias Assessment Tool.

Results

The present meta-analysis ultimately incorporated 15 studies comprising a total of 1,310 patients with RD. We pooled estimated the sensitivity, specificity as well as DOR from patient-based analyses with 0.89 (95% confidence interval [CI]: 0.84–0.93), 0.69 (95% CI: 0.59–0.77) and 18.0 (95% CI: 8.0–39.0), respectively. Moreover, we calculated the combined positive likelihood ratio (PLR) as well as negative likelihood ratio (NLR) to be 2.8 (95% CI: 2.1–3.9) and 0.16 (95% CI: 0.10–0.27), respectively. Additionally, area under the curve (AUC) of the summary receiver operating characteristic (SROC) was calculated as 0.88 (95% CI: 0.85–0.90) reflecting prognostic accuracy. Subsequently, subgroup analysis indicated that the clinical efficacy of C. sinensis in northern Chinese patients with RD was superior to that of southern. On the other hand, C. sinensis significantly reduced patients’ blood creatinine levels, shortened the oliguria period, and increased urine osmolality, indicating it can improve the function of glomeruli and renal tubules.

Conclusion

Our results indicate that C. sinensis can be considered a dependable clinical treatment for individuals with RD. It may improve the function of glomeruli and tubules, promote the recovery of tubular function, and thus enhance the clinical therapeutic effects.

Systematic review registration

www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier CRD42024559042.
Severe late onset capillary leak syndrome post allo-HSCT successfully treated by bevacizumab: a case report
Authors: Xue Song , Chen Jiaqi , Shi Yuzhu , Zhang Lina , Chen Man , Sun Huipeng , Cao Xingyu , Wu Fang
Published: January 2025

This article describes a case of successful management of late-onset CLS occurring after allo-HSCT, employing bevacizumab as the therapeutic agent. Capillary leak syndrome (CLS) represents a critical complication arising from allogeneic hematopoietic stem cell transplantation (allo-HSCT). The prognosis for CLS remains considerably constrained. A targeted therapeutic approach targeting vascular endothelial growth factor (VEGF) offers a novel strategy for the management of CLS.