bluuebunny/crossref_metadata_2025 huggingface dataset:
The Emerging Role of Extracellular Vesicle-Associated RNAs in the Multiple Myeloma Microenvironment
Authors: Khalife Jihane , Sanchez James F. , Pichiorri Flavia
Published: June 2021

Multiple myeloma (MM) is a cancer of terminally differentiated plasma cells (PCs) that develop at multiple sites within the bone marrow (BM). MM is treatable but rarely curable because of the frequent emergence of drug resistance and relapse. Increasing evidence indicates that the BM microenvironment plays a major role in supporting MM-PC survival and resistance to therapy. The BM microenvironment is a complex milieu containing hematopoietic cells, stromal cells, endothelial cells, immune cells, osteoclasts and osteoblasts, all contributing to the pathobiology of MM, including PC proliferation, escape from immune surveillance, angiogenesis and bone disease development. Small extracellular vesicles (EVs) are heterogenous lipid structures released by all cell types and mediate local and distal cellular communication. In MM, EVs are key mediators of the cross-talk between PCs and the surrounding microenvironment because of their ability to deliver bioactive cargo molecules such as lipids, mRNAs, non-coding regulatory RNA and proteins. Hence, MM-EVs highly contribute to establish a tumor-supportive BM niche that impacts MM pathogenesis and disease progression. In this review, we will first highlight the effects of RNA-containing, MM-derived EVs on the several cellular compartments within the BM microenvironment that play a role in the different aspects of MM pathology. We will also touch on the prospective use of MM-EV-associated non-coding RNAs as clinical biomarkers in the context of “liquid biopsy” in light of their importance as a promising tool in MM diagnosis, prognosis and prediction of drug resistance.
Validation of the Clinical Treatment Score Post–Five Years in Breast Cancer Patients for Predicting Late Distant Recurrence: A Single-Center Investigation in Korea
Authors: Lee Jun-Hee , Lee Se Kyung , Chae Byung Joo , Yu Jonghan , Lee Jeong Eon , Kim Seok Won , Nam Seok Jin , Ryu Jai Min
Published: June 2021

Background

Endocrine therapy is administered to hormone-positive breast cancer patients to prevent distant metastasis. It is important to evaluate the risk of recurrence and to determine which patients are viable candidates for such treatment because hormone therapy has side effects that can include postmenopausal symptoms. The Clinical Treatment Score post–five years (CTS5), a simple tool for identifying candidates for endocrine therapy, was recently introduced; however, CTS5 only has been applied in validation studies with postmenopausal women. We aimed to validate CTS5 among premenopausal breast cancer patients.

Methods

We identified patients treated between 1994 and 2014 at Samsung Medical Center in Seoul, Korea, and followed their treatment outcomes for more than 60 months after surgery using clinicopathologic parameters. According to menopausal status, we divided the study population into two groups: pre- and postmenopausal women. After calculating CTS5 values based on some parameters, we stratified the rate of late distant recurrence (DR) and analyzed the correlation between CTS5 value and late DR by risk.

Results

Among 16,904 patients treated surgically for breast cancer, 2,605 with hormone receptor–positive breast cancer who received endocrine therapy were included. Of these, 1,749 (67.14%) patients were premenopausal women, and the median age was 44.00 years. When categorizing study participants according to CTS5-related risk for late DR, 86.79% were categorized as low risk, 5.95% were categorized as intermediate risk, and 7.26% were categorized as high risk. The annual rate of DR was 1.41% for those in the present study and was similar between pre- and postmenopausal participants (1.40 vs. 1.42). Distant metastasis-free survival was not different between the two groups (hazard ratio: 0.817, 95% confidence interval [CI]: 0.547–1.221). The area under the receiver operating characteristic curve at 10 years for premenopausal and postmenopausal patients was 61.75 (95% CI: 52.97–70.53) and 72.71 (95% CIs: 63.30–82.12), respectively.

Conclusions

Although CTS5 was able to predict late DR, it should be applied with caution in premenopausal women. A CTS5 calculator for premenopausal women might be needed to not underestimate the risk of recurrence in Korea.
Baicalin Attenuates Oxygen–Glucose Deprivation/Reoxygenation–Induced Injury by Modulating the BDNF-TrkB/PI3K/Akt and MAPK/Erk1/2 Signaling Axes in Neuron–Astrocyte Cocultures
Authors: Li Changxiang , Sui Conglu , Wang Wei , Yan Juntang , Deng Nan , Du Xin , Cheng Fafeng , Ma Xiaona , Wang Xueqian , Wang Qingguo
Published: June 2021

Background: Baicalin (BCL), a candidate drug for ischemic stroke, has been indicated to protect neurons by promoting brain-derived neurotrophic factor (BDNF). However, the cellular source of BDNF release promoted by baicalin and its detailed protective mechanism after ischemia/reperfusion remains to be studied. The aim of this study was to investigate the neuroprotective mechanisms of baicalin against oxygen–glucose deprivation/reoxygenation (OGD/R) in a neuron–astrocyte coculture system and to explore whether the BDNF-TrkB pathway is involved.

Methods and Results: A neuron–astrocyte coculture system was established to elucidate the role of astrocytes in neurons under OGD/R conditions. The results demonstrated that astrocytes became reactive astrocytes and released more BDNF in the coculture system to attenuate neuronal apoptosis and injury after OGD/R. BCL maintained the characteristics of reactive astrocytes and obviously increased the expression of cyclic AMP response element-binding protein (CREB) and the levels of BDNF in the coculture system after OGD/R. To further verify whether BDNF binding to its receptor tyrosine kinase receptor B (TrkB) was required for the neuroprotective effect of baicalin, we examined the effect of ANA-12, an antagonist of TrkB, on NA system injury, including oxidative stress, inflammation, and apoptosis induced by OGD/R. The results showed that treatment of NA systems with ANA-12 significantly attenuated the neuroprotection of BCL. The phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) and mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathways are two important downstream cascades of signaling pathways activated by BDNF binding to TrkB. We investigated the expressions of TrkB, PI3K, Akt, MAPK, and ERK. The results demonstrated that baicalin significantly increased the expressions of TrkB, PI3K/AKT, and MAPK/ERK.

Conclusion: The neuroprotective effects of baicalin against oxidative stress, inflammation, and apoptosis were improved by astrocytes, mainly mediated by increasing the release of BDNF and its associated receptor TrkB and downstream signaling regulators PI3K/Akt and MAPK/ERK1/2.
Yanghe Decoction Suppresses the Experimental Autoimmune Thyroiditis in Rats by Improving NLRP3 Inflammasome and Immune Dysregulation
Authors: Ma Bing’e , Chen Dexuan , Liu Yangjing , Zhao Zhengping , Wang Jianhua , Zhou Guowei , Xu Kun , Zhu Taiyang , Wang Qiong , Ma Chaoqun
Published: June 2021

Inflammation is an important contributor to autoimmune thyroiditis. Yanghe decoction (YH) is a traditional Chinese herbal formulation which has various anti-inflammatory effects. It has been used for the treatment of autoimmune diseases such as ankylosing spondylitis In this study we aimed to investigate the effects of YH on autoimmune thyroiditis in a rat model and elucidate the underlying mechanisms. The experimental autoimmune thyroiditis (EAT) model was established by thyroglobulin (pTG) injections and excessive iodine intake. Thyroid lesions were observed using hematoxylin and eosin (H and E) staining and serum TgAb, TPOAb, TSH, T3, and T4 levels were measured by enzyme-linked immunosorbent assay IL-35 levels were evaluated using real-time polymerase chain reaction (RT-PCR) and Th17/Treg balance in peripheral blood mononuclear cells (PBMCs) was determined by flow cytometry and RT-PCR. Changes in Wnt/β-catenin signaling were evaluated using Western blot. Immunofluorescence staining and western blot were employed to examine NLRP3 inflammasome activation in the thyroid. YH minimized thyroid follicle injury and decreased concentrations of serum TgAb, TPOAb, TSH, T3, and T4 in EAT model. The mRNA of IL-35 was increased after YH treatment. YH also increased the percentage of Treg cells, and decreased Th17 proportion as well as Th17/Treg ratio in PBMCs. Meanwhile, the mRNA levels of Th17 related cytokines (RORγt, IL-17A, IL-21, and IL-22) were suppressed and Treg related cytokines (FoxP3, TGF-β, and IL-10) were promoted in PBMCs. Additionally, the protein expressions of Wnt-1 and β-catenin were unregulated after YH treatment. NLRP3 immunostaining signal and protein levels of IL-17, p-NF-κB, NLRP3, ASC, cleaved-Caspase-1, cleaved-IL-1β, and IL-18 were downregulated in the thyroid after YH intervention. Overall, the present study demonstrated that YH alleviated autoimmune thyroiditis in rats by improving NLRP3 inflammasome and immune dysregulation.
NQO1 Binds and Supports SIRT1 Function
Authors: Tsvetkov Peter , Adler Julia , Strobelt Romano , Adamovich Yaarit , Asher Gad , Reuven Nina , Shaul Yosef
Published: June 2021

Silent information regulator 2-related enzyme 1 (SIRT1) is an NAD+-dependent class III deacetylase and a key component of the cellular metabolic sensing pathway. The requirement of NAD+ for SIRT1 activity led us to assume that NQO1, an NADH oxidoreductase producing NAD+, regulates SIRT1 activity. We show here that SIRT1 is capable of increasing NQO1 (NAD(P)H Dehydrogenase Quinone 1) transcription and protein levels. NQO1 physically interacts with SIRT1 but not with an enzymatically dead SIRT1 H363Y mutant. The interaction of NQO1 with SIRT1 is markedly increased under mitochondrial inhibition. Interestingly, under this condition the nuclear pool of NQO1 is elevated. Depletion of NQO1 compromises the role of SIRT1 in inducing transcription of several target genes and eliminates the protective role of SIRT1 following mitochondrial inhibition. Our results suggest that SIRT1 and NQO1 form a regulatory loop where SIRT1 regulates NQO1 expression and NQO1 binds and mediates the protective role of SIRT1 during mitochondrial stress. The interplay between an NADH oxidoreductase enzyme and an NAD+ dependent deacetylase may act as a rheostat in sensing mitochondrial stress.
Quantifying Legal Entropy
Authors: Sichelman Ted
Published: June 2021

Many scholars have employed the term “entropy” in the context of law and legal systems to roughly refer to the amount of “uncertainty” present in a given law, doctrine, or legal system. Just a few of these scholars have attempted to formulate a quantitative definition of legal entropy, and none have provided a precise formula usable across a variety of legal contexts. Here, relying upon Claude Shannon's definition of entropy in the context of information theory, I provide a quantitative formalization of entropy in delineating, interpreting, and applying the law. In addition to offering a precise quantification of uncertainty and the information content of the law, the approach offered here provides other benefits. For example, it offers a more comprehensive account of the uses and limits of “modularity” in the law—namely, using the terminology of Henry Smith, the use of legal “boundaries” (be they spatial or intangible) that “economize on information costs” by “hiding” classes of information “behind” those boundaries. In general, much of the “work” performed by the legal system is to reduce legal entropy by delineating, interpreting, and applying the law, a process that can in principle be quantified.
Proportional Assist Ventilation Improves Leg Muscle Reoxygenation After Exercise in Heart Failure With Reduced Ejection Fraction
Authors: Borghi-Silva Audrey , Goulart Cassia da Luz , Carrascosa Cláudia R. , Oliveira Cristino Carneiro , Berton Danilo C. , de Almeida Dirceu Rodrigues , Nery Luiz Eduardo , Arena Ross , Neder J. Alberto
Published: June 2021

Background

Respiratory muscle unloading through proportional assist ventilation (PAV) may enhance leg oxygen delivery, thereby speeding off-exercise oxygen uptake (V.⁢O2) kinetics in patients with heart failure with reduced left ventricular ejection fraction (HFrEF).

Methods

Ten male patients (HFrEF = 26 ± 9%, age 50 ± 13 years, and body mass index 25 ± 3 kg m2) underwent two constant work rate tests at 80% peak of maximal cardiopulmonary exercise test to tolerance under PAV and sham ventilation. Post-exercise kinetics of V.⁢O2, vastus lateralis deoxyhemoglobin ([deoxy-Hb + Mb]) by near-infrared spectroscopy, and cardiac output (QT) by impedance cardiography were assessed.

Results

PAV prolonged exercise tolerance compared with sham (587 ± 390 s vs. 444 ± 296 s, respectively; p = 0.01). PAV significantly accelerated V.⁢O2 recovery (τ = 56 ± 22 s vs. 77 ± 42 s; p < 0.05), being associated with a faster decline in Δ[deoxy-Hb + Mb] and QT compared with sham (τ = 31 ± 19 s vs. 42 ± 22 s and 39 ± 22 s vs. 78 ± 46 s, p < 0.05). Faster off-exercise decrease in QT with PAV was related to longer exercise duration (r = −0.76; p < 0.05).

Conclusion

PAV accelerates the recovery of central hemodynamics and muscle oxygenation in HFrEF. These beneficial effects might prove useful to improve the tolerance to repeated exercise during cardiac rehabilitation.
TdP Incidence in Methoxamine-Sensitized Rabbit Model Is Reduced With Age but Not Influenced by Hypercholesterolemia
Authors: Nalos Lukáš , Jarkovská Dagmar , Švíglerová Jitka , Süß Annabell , Záleský Jakub , Rajdl Daniel , Krejčová Milada , Kuncová Jitka , Rosenberg Josef , Štengl Milan
Published: June 2021

Metabolic syndrome is associated with hypercholesterolemia, cardiac remodeling, and increased susceptibility to ventricular arrhythmias. Effects of diet-induced hypercholesterolemia on susceptibility to torsades de pointes arrhythmias (TdP) together with potential indicators of arrhythmic risk were investigated in three experimental groups of Carlsson’s rabbit model: (1) young rabbits (YC, young control, age 12–16 weeks), older rabbits (AC, adult control, age 20–24 weeks), and older age-matched cholesterol-fed rabbits (CH, cholesterol, age 20–24 weeks). TdP was induced by α-adrenergic stimulation by methoxamine and IKr block in 83% of YC rabbits, 18% of AC rabbits, and 21% of CH rabbits. High incidence of TdP was associated with high incidence of single (SEB) and multiple ectopic beats (MEB), but the QTc prolongation and short-term variability (STV) were similar in all three groups. In TdP-susceptible rabbits, STV was significantly higher compared with arrhythmia-free rabbits but not with rabbits with other than TdP arrhythmias (SEB, MEB). Amplitude-aware permutation entropy analysis of baseline ECG could identify arrhythmia-resistant animals with high sensitivity and specificity. The data indicate that the TdP susceptibility in methoxamine-sensitized rabbits is affected by the age of rabbits but probably not by hypercholesterolemia. Entropy analysis could potentially stratify the arrhythmic risk and identify the low-risk individuals.
Adaptation and Psychometric Evaluation of the Chinese Counseling Competencies Scale-Revised
Authors: Xia Wei , Li William Ho Cheung , Liang Tingna , Luo Yuanhui , Ho Laurie Long Kwan , Cheung Ankie Tan , Song Peige
Published: June 2021

Objectives: This study conducted a linguistic and psychometric evaluation of the Chinese Counseling Competencies Scale-Revised (CCS-R).

Methods: The Chinese CCS-R was created from the original English version using a standard forward-backward translation process. The psychometric properties of the Chinese CCS-R were examined in a cohort of 208 counselors-in-training by two independent raters. Fifty-three counselors-in-training were asked to undergo another counseling performance evaluation for the test-retest. The confirmatory factor analysis (CFA) was conducted for the Chinese CCS-R, followed by internal consistency, test-retest reliability, inter-rater reliability, convergent validity, and concurrent validity.

Results: The results of the CFA supported the factorial validity of the Chinese CCS-R, with adequate construct replicability. The scale had a McDonald's omega of 0.876, and intraclass correlation coefficients of 0.63 and 0.90 for test-retest reliability and inter-rater reliability, respectively. Significantly positive correlations were observed between the Chinese CCS-R score and scores of performance checklist (Pearson's γ = 0.781), indicating a large convergent validity, and knowledge on drug abuse (Pearson's γ = 0.833), indicating a moderate concurrent validity.

Conclusion: The results support that the Chinese CCS-R is a valid and reliable measure of the counseling competencies.

Practice implication: The CCS-R provides trainers with a reliable tool to evaluate counseling students' competencies and to facilitate discussions with trainees about their areas for growth.
Teacher Feedback Practices, Student Feedback Motivation, and Feedback Behavior: How Are They Associated With Learning Outcomes?
Authors: Gan Zhengdong , An Zhujun , Liu Fulan
Published: June 2021

In spite of much recent theorizing about teacher provision of feedback, relatively fewer studies look at the dynamic relationships between teacher feedback practices, student feedback experience, and their learning outcomes in higher education settings. To fill this gap, this study looked at 308 university students' perceived teacher feedback practices and their feedback experiences in an English Studies course context at a key and non-key university, and explored how teacher feedback, student feedback motivation and feedback behavior were associated with students' course satisfaction and course exam performance. Results showed that students from the key university reported a higher level of teacher feedback use as well as student feedback motivation and behavior. Structural equation modeling (SEM) suggested that in the case of the non-key university, student feedback behavior significantly predicted course satisfaction and course exam results; teacher feedback also indirectly influenced course satisfaction and course exam results. In the case of the key university, while teacher feedback and student feedback behavior each had significant influence on course satisfaction, student feedback behavior showed no direct significant effect on course exam results, and teacher feedback also showed no significant indirect influence on course exam results.
Extending the Population Health Workforce Through Service Learning Internships During COVID: A Community Case Study
Authors: Belkora Jeffrey , Weinberg Tia , Murphy Jasper , Karthikeyan Sneha , Tran Henrietta , Toliver Tasha , Lopez Freddie , Tominaga Grant , Helle Michael , Intinarelli Gina , Adler Joshua
Published: June 2021

This report arises from the intersection of service learning and population health at an academic medical center. At the University of California, San Francisco (UCSF), the Office of Population Health and Accountable Care (OPHAC) employs health care navigators to help patients access and benefit from high-value care. In early 2020, facing COVID-19, UCSF leaders asked OPHAC to help patients and employees navigate testing, treatment, tracing, and returning to work protocols. OPHAC established a COVID hotline to route callers to the appropriate resources, but needed to increase the capacity of the navigator workforce. To address this need, OPHAC turned to UCSF's service learning program for undergraduates, the Patient Support Corps (PSC). In this program, UC Berkeley undergraduates earn academic credit in exchange for serving as unpaid patient navigators. In July 2020, OPHAC provided administrative funding for the PSC to recruit and deploy students as COVID hotline navigators. In September 2020, the PSC deployed 20 students collectively representing 2.0 full-time equivalent navigators. After training and observation, and with supervision and escalation pathways, students were able to fill half-day shifts and perform near the level of staff navigators. Key facilitators relevant to success reflected both PSC and OPHAC strengths. The PSC onboards student interns as institutional affiliates, giving them access to key information technology systems, and trains them in privacy and other regulatory requirements so they can work directly with patients. OPHAC strengths included a learning health systems culture that fosters peer mentoring and collaboration. A key challenge was that, even after training, students required around 10 h of supervised practice before being able to take calls independently. As a result, students rolled on to the hotline in waves rather than all at once. Post-COVID, OPHAC is planning to use student navigators for outreach. Meanwhile, the PSC is collaborating with pipeline programs in hopes of offering this internship experience to more students from backgrounds that are under-represented in healthcare. Other campuses in the University of California system are interested in replicating this program. Adopters see the opportunity to increase capacity and diversity while developing the next generation of health and allied health professionals.
Assessment of Sprint Parameters in Top Speed Interval in 100 m Sprint—A Pilot Study Under Field Conditions
Authors: Seidl Thomas , Russomanno Tiago Guedes , Stöckl Michael , Lames Martin
Published: June 2021

Improving performances in sprinting requires feedback on sprint parameters such as step length and step time. However, these parameters from the top speed interval (TSI) are difficult to collect in a competition setting. Recent advances in tracking technology allows to provide positional data with high spatio-temporal resolution. This pilot study, therefore, aims to automatically obtain general sprint parameters, parameters characterizing, and derived from TSI from raw speed. In addition, we propose a method for obtaining the intra-cyclic speed amplitude in TSI. We analyzed 32 100 m-sprints of 7 male and 9 female athletes (18.9 ± 2.8 years; 100 m PB 10.55–12.41 s, respectively, 12.18–13.31 s). Spatio-temporal data was collected with a radio-based position detection system (RedFIR, Fraunhofer Institute, Germany). A general velocity curve was fitted to the overall speed curve (vbase), TSI (upper quintile of vbase values) was determined and a cosine term was added to vbase within TSI (vcycle) to capture the cyclic nature of speed. This allowed to derive TSI parameters including TSI amplitude from the fitted parameters of the cosine term. Results showed good approximation for vbase (error: 5.0 ± 1.0%) and for vcycle (2.0 ± 1.0%). For validation we compared spatio-temporal TSI parameters to criterion values from laser measurement (speed) and optoelectric systems (step time and step length) showing acceptable RMSEs for mean speed (0.08 m/s), for step time (0.004 s), and for step length (0.03 m). Top speed interval amplitude showed a significant difference between males (mean: 1.41 m/s) and females (mean: 0.71 m/s) and correlations showed its independence from other sprint parameters. Gender comparisons for validation revealed the expected differences. This pilot study investigated the feasibility of estimating sprint parameters from high-quality tracking data. The proposed method is independent of the data source and allows to automatically obtain general sprint parameters and TSI parameters, including TSI amplitude assessed here for the first time in a competition-like setting.
Attenuation of Mycoplasma hyopneumoniae Strain ES-2 and Comparative Genomic Analysis of ES-2 and Its Attenuated Form ES-2L
Authors: Li Zhenya , Wang Yingxin , Zhang Yanyan , Tang Xibiao , Wang Xiangru , Liu Wenhao , Qian Yulin , Zhu Yongwei , Chen Huanchun , Tan Chen
Published: June 2021

Mycoplasma hyopneumoniaecauses swine respiratory disease worldwide. Due to the difficulty of isolating and cultivatingM. hyopneumoniae, very few attenuated strains have been successfully isolated, which hampers the development of attenuated vaccines. In order to produce an attenuatedM. hyopneumoniaestrain, we used the highly virulentM. hyopneumoniaestrain ES-2, which was serially passagedin vitro200 times to produce the attenuated strain ES-2L, and its virulence was evidenced to be low in an animal experiment. In order to elucidate the mechanisms underlying virulence attenuation, we performed whole-genome sequencing of both strains and conducted comparative genomic analyses of strain ES-2 and its attenuated form ES-2L. Strain ES-2L showed three large fragment deletion regions including a total of 18 deleted genes, compared with strain ES-2. Analysis of single-nucleotide polymorphisms (SNPs) and indels indicated that 22 dels were located in 19 predicted coding sequences. In addition to these indels, 348 single-nucleotide variations (SNVs) were identified between strains ES-2L and ES-2. These SNVs mapped to 99 genes where they appeared to induce amino acid substitutions and translation stops. The deleted genes and SNVs may be associated with decreased virulence of strain ES-2L. Our work provides a foundation for further examining virulence factors ofM. hyopneumoniaeand for the development of attenuated vaccines.
Design and Preliminary Testing of a Magnetic Spring as an Energy-Storing System for Reduced Power Consumption of a Humanoid Arm
Authors: Rodríguez-León Jhon F. , Cervantes Ilse , Castillo-Castañeda Eduardo , Carbone Giuseppe , Cafolla Daniele
Published: June 2021

The increasing use of robots in the industry, the growing energy prices, and higher environmental awareness have driven research to find new solutions for reducing energy consumption. In additional, in most robotic tasks, energy is used to overcome the forces of gravity, but in a few industrial applications, the force of gravity is used as a source of energy. For this reason, the use of magnetic springs with actuators may reduce the energy consumption of robots performing trajectories due their high-hardness magnetic properties of energy storage. Accordingly, this paper proposes a magnetic spring configuration as an energy-storing system for a two DoF humanoid arm. Thus, an integration of the magnetic spring system in the robot is described. A control strategy is proposed to enable autonomous use. In this paper, the proposed device is modeled and analyzed with simulations as: mechanical energy consumption and kinetic energy rotational and multibody dynamics. Furthermore, a prototype was manufactured and validated experimentally. A preliminary test to check the interaction between the magnetic spring system with the mechanism and the trajectory performance was carried out. Finally, an energy consumption comparison with and without the magnetic spring is also presented.
Impact of Camera Viewing Angle for Estimating Leaf Parameters of Wheat Plants from 3D Point Clouds
Authors: Li Minhui , Shamshiri Redmond R. , Schirrmann Michael , Weltzien Cornelia
Published: June 2021

Estimation of plant canopy using low-altitude imagery can help monitor the normal growth status of crops and is highly beneficial for various digital farming applications such as precision crop protection. However, extracting 3D canopy information from raw images requires studying the effect of sensor viewing angle by taking into accounts the limitations of the mobile platform routes inside the field. The main objective of this research was to estimate wheat (Triticum aestivum L.) leaf parameters, including leaf length and width, from the 3D model representation of the plants. For this purpose, experiments with different camera viewing angles were conducted to find the optimum setup of a mono-camera system that would result in the best 3D point clouds. The angle-control analytical study was conducted on a four-row wheat plot with a row spacing of 0.17 m and with two seeding densities and growth stages as factors. Nadir and six oblique view image datasets were acquired from the plot with 88% overlapping and were then reconstructed to point clouds using Structure from Motion (SfM) and Multi-View Stereo (MVS) methods. Point clouds were first categorized into three classes as wheat canopy, soil background, and experimental plot. The wheat canopy class was then used to extract leaf parameters, which were then compared with those values from manual measurements. The comparison between results showed that (i) multiple-view dataset provided the best estimation for leaf length and leaf width, (ii) among the single-view dataset, canopy, and leaf parameters were best modeled with angles vertically at −45° and horizontally at 0° (VA −45, HA 0), while (iii) in nadir view, fewer underlying 3D points were obtained with a missing leaf rate of 70%. It was concluded that oblique imagery is a promising approach to effectively estimate wheat canopy 3D representation with SfM-MVS using a single camera platform for crop monitoring. This study contributes to the improvement of the proximal sensing platform for crop health assessment.
Pigment Production under Cold Stress in the Green Microalga Chlamydomonas reinhardtii
Authors: Potijun Supakorn , Yaisamlee Chonlada , Sirikhachornkit Anchalee
Published: June 2021

Microalgae have long been used for the commercial production of natural colorants such as carotenoids and chlorophyll. Due to the rising demand for carotenoids and other natural products from microalgae, strategies to increase production efficiency are urgently needed. The production of microalgal biorefineries has been limited to countries with moderate climates. For countries with cooler climates and less daylight, methodologies for the efficient production of microalgal biorefineries need to be investigated. Algal strains that can be safely consumed as whole cells are also attractive alternatives for developing as carotenoid supplements, which can also contain other compounds with health benefits. Using such strains helps to eliminate the need for hazardous solvents for extraction and several other complicated steps. In this study, the mesophilic green alga Chlamydomonas reinhardtii was employed to study the effects of cold stress on cell physiology and the production of pigments and storage compounds. The results showed that temperatures between 10 and 20 °C induced carotenoid and chlorophyll accumulation in the wild-type strain of C. reinhardtii. Interestingly, the increased level of carotenoids suggested that they might play a crucial role in cold stress acclimation. A temperature of 15 °C resulted in the highest carotenoid and chlorophyll productivity. At this temperature, carotenoid and chlorophyll productivity was 2 times and 1.3 times higher than at 25 °C, respectively. Subjecting a mutant defective in lutein and zeaxanthin accumulation to cold stress revealed that these two carotenoids are not essential for cold stress survival. Therefore, cold temperature could be used as a strategy to induce and increase the productivity of pigments in C. reinhardtii.
Impact of Climate Change on Rice Yield in Malaysia: A Panel Data Analysis
Authors: Tan Boon Teck , Fam Pei Shan , Firdaus R. B. Radin , Tan Mou Leong , Gunaratne Mahinda Senevi
Published: June 2021

Climate change is a global problem since many countries worldwide are becoming increasingly vulnerable to natural disasters. Numerous climate models in various studies project a decline in agricultural productivity that will mainly be due to excessive heat in tropical and subtropical regions, especially in Southeast Asia. As a Southeast Asian country, Malaysia is no exception to this problem. Hence, the present study aimed to examine the impact of climate change on rice yields in Malaysia. A panel data approach was adopted using data from 1987 to 2017 on eight granary areas in Peninsular Malaysia. The main objectives were to assess the impact of climate variables (i.e., minimum and maximum temperature and precipitation) on rice yield and the variance of the impact during the main season and off-season. Our regression results indicate that precipitation was not statistically significant in all model specifications for both the main and off-season. While the maximum temperature was found to be negatively associated with yield during the off-season, the minimum temperature showed a positive effect in both cropping seasons. We used the HadGEM3-GC31 N512 resolution model based on the high-emission Shared Socioeconomic Pathways 8.5 scenario (SSPs-8.5) from the High-Resolution Model Intercomparison Project (HighResMIP) of the Coupled Model Intercomparison Project Phase 6 (CMIP6) to project future climate change in 2030 and 2040. The projected results indicate that rice yield would show a more positive trend by 2040 when compared to the previous decade, ranging from −0.02 to 19.85% during the main season and −2.77 to 7.41% during the off-season. Although rice yield is likely to increase in certain areas, other areas are projected to experience negative effects. Hence, adaptation at the farm level remains crucial, specifically during the off-season, since climate change could widen the gaps in rice yields between cropping seasons and among granary areas.
IoFarm in Field Test: Does a Cost-Optimal Choice of Fertilization Influence Yield, Protein Content, and Market Performance in Crop Production?
Authors: Tröster Michael Friedrich , Sauer Johannes
Published: June 2021

Decision-support system (DSS) IoFarm was developed to identify economically optimal fertilizer strategies on the farm level. The average cost savings are 66 EUR ha−1. This study aimed to determine whether this approach impacts yield, protein content, and market performance in crop production compared to usual farm-fertilization strategies. Few DSSs for fertilizer optimization consider multiple nutrients. DSSs with a clear focus on both fertilizer intensity and the least-cost combination of fertilizers are even rarer. To the best of our knowledge, there is no information in the literature on the impact of such DSSs on yield, protein content, and market performance for cereal–maize crop rotation. This study determines for the first time whether the financial benefits of using such an optimization tool are in conflict with important agronomic goals. In a three-year field trial, IoFarm was compared to standard farm-fertilization strategies. Results were evaluated with an analysis of variance followed by post hoc tests. No significant differences in yield, protein content, and market performance were found for comparable fertilization variants (with or without organic fertilization). However, differences exist in the selection of fertilizers and the timing of fertilization. Results show the agronomic comparability of IoFarm and usual farm-fertilizer strategies.
Phytohormonal and Transcriptomic Response of Hulless Barley Leaf in Response to Powdery Mildew Infection
Authors: Sang Zha , Zhang Minjuan , Mu Wang , Yang Haizhen , Yang Chunbao , Xu Qijun
Published: June 2021

Powdery mildew (PM) caused by Blumeria graminis (DC.) Golovin ex Speer f. sp. hordei Marchal (Bgh) is one of the major yield reducing diseases in hulless barley (Hordeum vulgare L. var. nudum Hook. f.). Genotypes with contrasting resistance to PM offer unique opportunities to explore the transcriptome in order to understand the expression changes in genes and pathways. In this study, we explored the phytohormone levels and transcriptome of a Bgh susceptible (XL19) and resistant (ZYM1288) hulless barley genotypes at 0, 5, 12, 24, and 36 h post infection (hpi) with Bgh. We found relatively higher levels of abscisic acid, jasmonic acid, salicylic acid, and cytokinins in ZYM1288. The transcriptome analyses identified 31,354 genes that were enriched in signaling, energy, and defense related pathways. Higher numbers of differentially expressed genes (DEGs) were found in XL19 as compared to ZYM1288 after 5 (3603 vs. 2341) and 12 hpi (3530 vs. 2416). However, after 24 and 36 hpi, the number of DEGs was higher in ZYM1288 as compared to XL19 i.e., 3625 vs. 3034 and 5855 vs. 2725, respectively. Changes in hormone levels drove downstream expression changes in plant-hormone signaling that helped ZYM1288 to perform better under Bgh infection. The expression of DEGs in MAPK-signaling and Toll-like receptor signaling pathways, glucosinolate biosynthesis, glutathione metabolism, brassinosteroid metabolism, and energy related pathways indicated their common roles in defense against PM. Key genes related to PM-resistance were upregulated in the resistant genotype. These genes provide key information towards differences in both genotypes towards resistance to PM. The transcriptomic signatures explored in this study will broaden our understanding towards molecular regulation of resistance to PM in hulless barley.
An Investigation into the Influence of Different Types of Nesting Materials upon the Welfare of Captive Chimpanzees (Pan troglodytes)
Authors: Anderson Naomi , Amarasekaran Bala , Riba David
Published: June 2021

Ensuring the welfare of captive chimpanzees (Pan troglodytes) is crucial, in part because they can act as a conservation resource in the case of wild populations becoming extinct. One strategy often adopted to ensure animal welfare is environmental enrichment. In this study, we investigated the impact of different nesting materials (leaves and branches, long grass, cotton sheets, and shredded newspaper) upon the welfare of chimpanzees housed at Tacugama Chimpanzee Sanctuary (Sierra Leone). Data was collected on 20 subjects (aged 4 to 15 years) between November 2019 and March 2020. Welfare was based on individuals’ relative frequency of affiliative, abnormal and agonistic behaviours, as well as their social and behavioural competence. We found that individuals’ welfare was higher when presented with nesting materials (compared to the control and post-treatment conditions), particularly when given shredded newspaper, regardless of whether the material was presented separately or in conjunction with another. In addition, welfare was highest: during the morning (vs evening); in groups of older individuals (vs younger); with females engaging in less agonistic behaviours compared to males; and males displaying relatively higher behavioural competence. Our results support previous research that captive chimpanzees be supplied with destructible nesting materials, and demonstrate that synthetic enrichments can indeed have a more positive impact upon welfare than their natural alternatives.
The Comparison of Latero-Medial versus Dorso-Palmar/Plantar Drilling for Cartilage Removal in the Proximal Interphalangeal Joint
Authors: Spadari Alessandro , Forni Giulia , Del Magno Sara , Tagliavia Claudio , Canova Marco , Grandis Annamaria , Rinnovati Riccardo
Published: June 2021

The aims of the present study were to compare the percentages of articular cartilage removed using a lateral drilling approach of the proximal interphalangeal joint (PIPJ) and a dorsal drilling approach, and to assess the usefulness of digital fluoroscopy when performing a lateral drilling approach. Sixty cadaveric PIPJs were drilled using a surgical drill bit to remove the articular cartilage. The limbs were divided into three groups containing 10 forelimbs and 10 hindlimbs each. One group received the dorsal drilling approach, the second one received the lateral drilling approach and the last one received the lateral drilling approach under digital fluoroscopy guidance. The percentage of articular cartilage removed from each articular surface was assessed using Adobe Photoshop ® software. The percentages of removed cartilage turned out to be significantly higher with lateral approach, especially under fluoroscopic guidance, both in the forelimbs (p = 0.00712) and hindlimbs (p = 0.00962). In conclusion, the lateral drilling approach seems to be a minimally invasive technique with which to perform PIPJ arthrodesis, even more efficient than the previously reported dorsal approach.
Nerolidol Attenuates Oxidative Stress, Inflammation, and Apoptosis by Modulating Nrf2/MAPK Signaling Pathways in Doxorubicin-Induced Acute Cardiotoxicity in Rats
Authors: Arunachalam Seenipandi , Nagoor Meeran M. F. , Azimullah Sheikh , Sharma Charu , Goyal Sameer N. , Ojha Shreesh
Published: June 2021

The clinical usage of doxorubicin (DOX), a potent anthracycline antineoplastic drug, is often limited by its cardiotoxic effects. Thus, for improving usage of DOX, the aim of this study was to assess the cardioprotective effects of nerolidol (NERO) in a rat model of DOX-induced acute cardiotoxicity and examine underlying molecular mechanisms that contribute to these effects. To induce acute cardiotoxicity male albino Wistar rats were injected with single dose intraperitoneal DOX (12.5 mg/kg). The rats were treated with NERO (50 mg/kg, orally) for five days. DOX-injected rats showed elevated levels of cardiac marker enzymes and enhanced oxidative stress markers along with altered Nrf2/Keap1/HO-1 signaling pathways. DOX administration also induced the activation of NF-κB/MAPK signaling and increased the levels and expression of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) as well as expression of inflammatory mediators (iNOS and COX-2) in the heart. DOX also triggered DNA damage and apoptotic cell death in the myocardium. Additionally, histological studies revealed structural alterations of the myocardium. NERO treatment exhibited protection against the deleterious results of DOX on myocardium, as evidenced by the restoration of altered biochemical parameters, mitigated oxidative stress, inflammation, and apoptosis. The findings of the present study demonstrate that NERO provides cardioprotective effects against DOX-induced acute cardiotoxicity attributed to its potent antioxidant, anti-inflammatory, and antiapoptotic activities through modulating cellular signaling pathways.
Iron and Potassium Fertilization Improve Rocket Growth without Affecting Tilapia Growth and Histomorphology Characteristics in Aquaponics
Authors: Stathopoulou Paraskevi , Tsoumalakou Evangelia , Levizou Efi , Vanikiotis Theofilos , Zaoutsos Stefanos , Berillis Panagiotis
Published: June 2021

Aquaponics provides an alternative opportunity for the combined production of fish and plants. Most of the essential nutrients required for optimal plant growth can be supplied from the fish feed, except for K and Fe. These nutrients are usually inadequate in fish feed. In this study, red tilapia and rocket plants were co-cultivated in an aquaponics system along with the extra addition of K and Fe. Fish growth, morpho-anatomical characteristics, and K and Fe loading on fish gills were studied. Plant growth parameters were also determined. The addition of Fe and K slightly improved the produced fresh biomass of rocket per unit area and had no impact on tilapia growth and survival. No severe histological alterations in fish gills, liver, and midgut were detected. EDX analysis showed that the addition of K led to the enrichment of this specific ion in fish gills, but no effects of this accumulation were found on other aspects of fish growth and survival.
Linking Soil Erosion Modeling to Landscape Patterns and Geomorphometry: An Application in Crete, Greece
Authors: Brini Imen , Alexakis Dimitrios D. , Kalaitzidis Chariton
Published: June 2021

Soil erosion is a severe and continuous environmental problem caused mainly by natural factors, which can be enhanced by anthropogenic activities. The morphological relief with relatively steep slopes, the dense drainage network, and the Mediterranean climate are some of the factors that render the Paleochora region (South Chania, Crete, Greece) particularly prone to soil erosion in cases of intense rainfall events. In this study, we aimed to assess the correlation between soil erosion rates estimated from the Revised Universal Soil Loss Equation (RUSLE) and the landscape patterns and to detect the most erosion-prone sub-basins based on an analysis of morphometric parameters, using geographic information system (GIS) and remote sensing technologies. The assessment of soil erosion rates was conducted using the RUSLE model. The landscape metrics analysis was carried out to correlate soil erosion and landscape patterns. The morphometric analysis helped us to prioritize erosion-prone areas at the sub-basin level. The estimated soil erosion rates were mapped, showing the spatial distribution of the soil loss for the study area in 2020. For instance, the landscape patterns seemed to highly impact the soil erosion rates. The morphometric parameter analysis is considered as a useful tool for delineating areas that are highly vulnerable to soil erosion. The integration of three approaches showed that there is are robust relationships between soil erosion modeling, landscape patterns, and morphometry.
Vermicomposting: A Valorization Alternative for Corn Cob Waste
Authors: Castillo-González Eduardo , De Medina-Salas Lorena , Giraldi-Díaz Mario Rafael , Sánchez-Noguez Cipriano
Published: June 2021

As vermicomposting has become a viable alternative for the valorization of organic waste; the objectives of this research were to (1) assess the feasibility of said process for corn cob waste (corn cobs and corn husks) and (2) evaluate the operation conditions for the biodegradation of different mixtures with load material (LM). LM did not include animal excreta as a nitrogen source, a practice widely used in a range of studies. The experiment consisted of an initial phase of pre-composting in order to obtain a partially stabilized substrate. Subsequently, four separate mixtures were made consisting of corn cob waste mixed with consistent load material (LM) containing vegetable waste and eggshells (CR, M1, M2, M3) to obtain a balance substrate able to facilitate degradation using Eisenia fetida earthworms. The following parameters were analyzed during the control process: temperature, pH, humidity, organic material (OM), total organic carbon (TOC), total nitrogen (TN) and carbon/nitrogen (C/N) ratio. The analysis of the final values of the stabilized mixtures showed that vermicomposting is indeed a feasible alternative for the degradation of corn cob waste for use as a soil improver.
An Advanced Spectral–Spatial Classification Framework for Hyperspectral Imagery Based on DeepLab v3+
Authors: Si Yifan , Gong Dawei , Guo Yang , Zhu Xinhua , Huang Qiangsheng , Evans Julian , He Sailing , Sun Yaoran
Published: June 2021

DeepLab v3+ neural network shows excellent performance in semantic segmentation. In this paper, we proposed a segmentation framework based on DeepLab v3+ neural network and applied it to the problem of hyperspectral imagery classification (HSIC). The dimensionality reduction of the hyperspectral image is performed using principal component analysis (PCA). DeepLab v3+ is used to extract spatial features, and those are fused with spectral features. A support vector machine (SVM) classifier is used for fitting and classification. Experimental results show that the framework proposed in this paper outperforms most traditional machine learning algorithms and deep-learning algorithms in hyperspectral imagery classification tasks.