bluuebunny/crossref_metadata_2025 huggingface dataset:
Revisiting Cattle Temperament in Beef Cow-Calf Systems: Insights from Farmers’ Perceptions about an Autochthonous Breed
Authors: Estévez-Moreno Laura X. , Miranda-de la Lama Genaro C. , Villarroel Morris , García Laura , Abecia José Alfonso , Santolaria Pilar , María Gustavo A.
Published: January 2021

Understanding temperament is an important part of cattle production since undesirable temperament may cause serious problems associated with aggression, maternal care, and human safety. However, little is known about how farmers define or assess temperament, especially in autochthonous cattle breeds. The aim of this study was to explore perceptions of farmers about the temperament of the Pyrenean cattle breed with special attention to beef cow-calf systems in Spain. The methodology used to obtain the information was focus group discussions (FGD). Farmers defined temperament as a behavioural response to challenging situations imposed by human handling. Specific terms used were related to active or passive reactions to fear (e.g., “strong”, “aggressive”, “nervous”, “fearful”). The speed of response to stimuli was also important. Female temperament was thought to become more docile with age while bull temperament was more variable. Maternal aggressiveness was highlighted as a potential human safety problem, but also desirable in an extensively bred animal who may need to defend calves against predators. Anatomical characteristics were seen as unreliable predictors of temperament, while behavioural indicators were more widely used, such as “alertness”, which was a general trait of the breed, and “gaze”, which, when associated with an alert expression, suggests a potential threat. Sensory acuity, such as sight and smell, were thought to be related with temperament in some FGDs but there was no overall agreement as to whether different behavioural responses were due to differences in sensory acuity. The results from the study could be useful during training programs or in the development of new genetic selection schemes and evaluation protocols involving cattle temperament.
Does the Effect of Replacing Cottonseed Meal with Dried Distiller’s Grains on Nellore Bulls Finishing Phase Vary between Pasture and Feedlot?
Authors: Hoffmann Alvair , Berça Andressa Scholz , Cardoso Abmael da Silva , Fonseca Natalia Vilas Boas , Silva Maria Luísa Curvelo , Leite Rhaony Gonçalves , Ruggieri Ana Cláudia , Reis Ricardo Andrade
Published: January 2021

The study aimed to evaluate the effect of replacing cottonseed meal by dried distiller’s grains (DDG) in terms of efficiency in the productive aspects of beef cattle finishing in pasture versus feedlot. The experiment was conducted in a completely randomized design in a 2 × 3 factorial arrangement, with two production systems (pasture versus feedlot) and three supplements: CM, conventional supplement with cottonseed meal (CM) as a protein source; 50DDG: supplement with 50% replacement of CM by DDG; and 100DDG: 100% replacement. The effect of replacing CM by DDG on dry matter and nutrients intake and nutrients digestibility depends on the finishing system (p < 0.05). While in the pasture system animal consumed more nutrients in the CM, a greater intake was observed in the 100DDG in feedlot. The nutrients digestibility was lower in the pasture (p < 0.05). Animal performance and final body weight were higher in the feedlot (p < 0.0001), with averages of 1.57 kg/d and 566 kg of final body weight (FBW) for feedlot, and 0.99 kg/d and 504 kg FBW for pasture. The use of DDG does not change the animal performance finished in pasture or feedlot, and it is a viable alternative to replace conventional supplements in finishing phase in both systems in tropical environment.
Accuracy of Imputation of Microsatellite Markers from a 50K SNP Chip in Spanish Assaf Sheep
Authors: Marina Héctor , Suarez-Vega Aroa , Pelayo Rocío , Gutiérrez-Gil Beatriz , Reverter Antonio , Esteban-Blanco Cristina , Arranz Juan José
Published: January 2021

Transitioning from traditional to new genotyping technologies requires the development of bridging methodologies to avoid extra genotyping costs. This study aims to identify the optimum number of single nucleotide polymorphisms (SNPs) necessary to accurately impute microsatellite markers to develop a low-density SNP chip for parentage verification in the Assaf sheep breed. The accuracy of microsatellite marker imputation was assessed with three metrics: genotype concordance (C), genotype dosage (length r2), and allelic dosage (allelic r2), for all imputation scenarios tested (0.5–10 Mb microsatellite flanking SNP windows). The imputation accuracy for the three metrics analyzed for all haplotype lengths tested was higher than 0.90 (C), 0.80 (length r2), and 0.75 (allelic r2), indicating strong genotype concordance. The window with 2 Mb length provides the best accuracy for the imputation procedure and the design of an affordable low-density SNP chip for parentage testing. We additionally evaluated imputation performance under two null models, naive (imputing the most common allele) and random (imputing by randomly selecting the allele), which in comparison showed weak genotype concordances (0.41 and 0.15, respectively). Therefore, we describe a precise methodology in the present article to impute multiallelic microsatellite genotypes from a low-density SNP chip in sheep and solve the problem of parentage verification when different genotyping platforms have been used across generations.
Riboswitches as Drug Targets for Antibiotics
Authors: Panchal Vipul , Brenk Ruth
Published: January 2021

Riboswitches reside in the untranslated region of RNA and regulate genes involved in the biosynthesis of essential metabolites through binding of small molecules. Since their discovery at the beginning of this century, riboswitches have been regarded as potential antibacterial targets. Using fragment screening, high-throughput screening and rational ligand design guided by X-ray crystallography, lead compounds against various riboswitches have been identified. Here, we review the current status and suitability of the thiamine pyrophosphate (TPP), flavin mononucleotide (FMN), glmS, guanine, and other riboswitches as antibacterial targets and discuss them in a biological context. Further, we highlight challenges in riboswitch drug discovery and emphasis the need to develop riboswitch specific high-throughput screening methods.
Impact of Innovative Technologies on the Content of Vitamin C and Its Bioavailability from Processed Fruit and Vegetable Products
Authors: Mieszczakowska-Frąc Monika , Celejewska Karolina , Płocharski Witold
Published: January 2021

Nowadays, thermal treatments are used for extending the shelf-life of vegetable and fruit products by inactivating microorganisms and enzymes. On the other hand, heat treatments often induce undesirable changes in the quality of the final product, e.g., losses of nutrients, color alterations, changes in flavor, and smell. Therefore, the food industry is opening up to new technologies that are less aggressive than thermal treatment to avoid the negative effects of thermal pasteurization. Non-thermal processing technologies have been developed during the last decades as an alternative to thermal food preservation. Processing changes the structure of fruit and vegetables, and hence the bioavailability of the nutrients contained in them. In this review, special attention has been devoted to the effects of modern technologies of fruit and vegetable processing, such as minimal processing (MPFV), high-pressure processing (HPP), high-pressure homogenization (HPH), ultrasounds (US), pulsed electric fields (PEF), on the stability and bioavailability of vitamin C.
The Demonstration of S2P (Serial-to-Parallel) Converter with Address Allocation Method Using 28 nm CMOS Technology
Authors: Kim Min-Su , Yang Youngoo , Koo Hyungmo , Oh Hansik
Published: January 2021

To improve the performance of analog, RF, and digital integrated circuits, the cutting-edge advanced CMOS technology has been widely utilized. We successfully designed and implemented a high-speed and low-power serial-to-parallel (S2P) converter for 5G applications based on the 28 nm CMOS technology. It can update data easily and quickly using the proposed address allocation method. To verify the performances, an embedded system (NI-FPGA) for fast clock generation on the evaluation board level was also used. The proposed S2P converter circuit shows extremely low power consumption of 28.1 uW at 0.91 V with a core die area of 60 × 60 μm2 and operates successfully over a wide clock frequency range from 5 M to 40 MHz.
Energy-Efficient Envelope Design for Apartment Blocks—Case Study of A Residential Building in Spain
Authors: Sánchez de la Flor Francisco José , Rodríguez Jara Enrique Ángel , Ruiz Pardo Álvaro , Salmerón Lissén José Manuel , Kolokotroni Maria
Published: January 2021

Buildings are known to be responsible for about a third of energy consumption in developed countries. This situation, together with the fact that the existing building stock is being renovated at a very slow pace, makes it crucial to focus on the energy retrofitting of buildings as the only way to reduce their contribution to these energy consumptions and the consequences derived from them in terms of pollution and climate change. The same level of insulation and the same type of windows is usually proposed for all dwellings in a building block. This article shows that since the improvements required by each dwelling in the same block are different, the proposed solution must also be different. The methodology is proposed for a practical case consisting of an apartment block in Cádiz, a demonstration building of the European RECO2ST project. To achieve the optimum solution for each case, a multi-objective optimization problem is solved: to minimize the annual heating demand of the building and the standard deviation of the annual demand of the different dwellings. Thanks to the use of the proposed methodology, it is possible to bring the building to a Nearly Zero Energy Building (NZEB) level, while avoiding excessive insulation that causes overheating in summer.
Recent Advances in Nanoparticle-Mediated Treatment of Inflammatory Bowel Diseases
Authors: Nedelcu Andreea , Mosteanu Ofelia , Pop Teodora , Mocan Teodora , Mocan Lucian
Published: January 2021

There have been continuous advances in nanoscience since the beginning of the 21st century, and the emerging field of computational nanomedicine, the development of nanomaterial-based sensors or the prominent biomedical engineering applications should be mentioned. Intestinal disorders causing prolonged inflammation of the digestive tract, largely known as inflammatory bowel disease (IBD), include Crohn’s disease (CD) and ulcerative colitis (UC), have seen a significant increase in incidence rates. Nanoparticle-based approaches to locally target therapy could help regulate immune responses and act as an anti-inflammatory in individual patients diagnosed with IBD. The results of the paper emphasize the major role that nanoparticle-mediated drug delivery has in IBD treatment, giving IBD patients in remission the chance for a more effective drug therapy with a decreased medication load.
Medical Applications of the GEMPix
Authors: Leidner Johannes , Murtas Fabrizio , Silari Marco
Published: January 2021

The GEMPix is a small gaseous detector with a highly pixelated readout, consisting of a drift region, three Gas Electron Multipliers (GEMs) for signal amplification, and four Timepix ASICs with 55 µm pixel pitch and a total of 262,144 pixels. A continuous flow of a gas mixture such as Ar:CO2:CF4, Ar:CO2 or propane-based tissue equivalent gas is supplied externally at a rate of 5 L/h. This article reviews the medical applications of the GEMPix. These include relative dose measurements in conventional photon radiation therapy and in carbon ion beams, by which on-line 2D dose images provided a similar or better performance compared to gafchromic films. Depth scans in a water phantom with 12C ions allowed measuring the 3D energy deposition and reconstructing the Bragg curve of a pencil beam. Microdosimetric measurements performed in neutron and photon fields allowed comparing dose spectra with those from Tissue Equivalent Proportional Counters and, additionally, to obtain particle track images. Some preliminary measurements performed to check the capabilities as the detector in proton tomography are also illustrated. The most important on-going developments are: (1) a new, larger area readout to cover the typical maximum field size in radiation therapy of 20 × 20 cm2; (2) a sealed and low-pressure version to facilitate measurements and to increase the equivalent spatial resolution for microdosimetry; (3) 3D particle track reconstruction when operating the GEMPix as a Time Projection Chamber.
Parametric Study of the Corrosion of API-5L-X65 QT Steel Using Potentiostat Based Measurements in a Flow Loop
Authors: Ravikumar Arjun , Rostron Paul , Vahdati Nader , Shiryayev Oleg
Published: January 2021

Low-carbon steel is widely used in industrial pipelines, and corrosion studies are focused mostly on erosion-corrosion, its prediction and control. In this paper, the corrosion rate in pipelines is modeled using a flow loop and measured by linear polarization resistance method (LPR) using a 3-electrode corrosion setup for API-5L-X65 QT steel. Optical microscopy and SEM studies are conducted to examine the surface of the sample and the corrosion products. The effect of NaCl concentration on the corrosion rate is studied at different pH, temperature range, and flow velocities with dissolved oxygen content in the solution maintained at 6 mg/L (6ppm). The corrosion rate is found to be varying from 1 mil per year (0.0254 mmyr−1) to 10 mils per year (0.254 mmyr−1), and the corrosion rate increases with the flow velocity and reaches a maximum at Reynolds Number above 10,000. Further increase in fluid velocity shows corrosion is flow insensitive, and uniform corrosion is predominant in the region.
Method for Preparation of a Candidate Reference Material of PM10 and PM2.5 Airborne Particulate Filters Loaded with Incineration Ash-Inter Comparison Results for Metal Concentrations
Authors: Bescond Alexandre , Oster Caroline , Fisicaro Paola , Goddard Sharon , Quincey Paul , Tsakanika Lamprini-Areti , Lymperopoulou Theopisti , Ochsenkuehn-Petropoulou Maria
Published: January 2021

Air pollution is an important issue that can have significant implications for human health. Consequently, air quality control is matter of great interest, and the ΕU has established strict legislation with respect to public health protection. A work package of the EMPIR project AEROMET focused on the investigation of traceable validated methods for chemical composition of airborne particulate matter (PM), including heavy metals. Incineration ash typically contains quantities of heavy and toxic metals in excess of the limits imposed for airborne PM, so it provides a candidate source for a Standard Reference Material (SRM). In this work, a method for loading incinerator ash (PM10 and PM2.5 fractions) on quartz filters with a good reproducibility and homogeneity was developed. An intercomparison exercise involving three separate laboratories was conducted for the elemental analysis of the prepared candidate reference material. The filters were treated by acid digestion and analyzed by Inductively Coupled Plasma–Mass Spectrometry (ICP-MS) according to the European standard EN 14902:2005. Eleven elements, including the regulated metals (As, Cd, Ni and Pb), were determined using different ICP-MS instruments and standardization methods. Data evaluation showed a good agreement between the results, with deviations below 10–15%. The development of a Standard Reference Material seems auspicious.
Changes in Air Temperature and Snow Cover in Winter in Poland
Authors: Tomczyk Arkadiusz M. , Bednorz Ewa , Szyga-Pluta Katarzyna
Published: January 2021

The primary objective of the paper was to characterize the climatic conditions in the winter season in Poland in the years 1966/67–2019/20. The study was based on daily values of minimum (Tmin) and maximum air temperature (Tmax), and daily values of snow cover depth. The study showed an increase in both Tmin and Tmax in winter. The most intensive changes were recorded in north-eastern and northern regions. The coldest winters were recorded in the first half of the analyzed multiannual period, exceptionally cold being winters 1969/70 and 1984/85. The warmest winters occurred in the second half of the analyzed period and among seasons with the highest mean Tmax, particularly winters 2019/20 and 1989/90 stood out. In the study period, a decrease in snow cover depth statistically significant in the majority of stations in Poland was determined, as well as its variability both within the winter season and multiannual.
Investigation of Resonance-Enhanced High-Order Harmonics by Two-Component Laser-Produced Plasmas
Authors: Liang Jingguang , Venkatesh Mottamchetty , Boltaev Ganjaboy S. , Ganeev Rashid A. , Lai Yu Hang , Guo Chunlei
Published: January 2021

Resonance-enhanced harmonics from laser-produced plasma plumes are an interesting phenomenon, whose underlying mechanism is still under debate. In particular, it is unclear whether the macroscopic dispersion properties of the plasma are the key factors for the formation of the enhancement. To shed light on this problem, we perform experiments with two-component plasmas, in which one of the components (tin) is known to be able to generate enhanced harmonics and the other component (lead) is known for altering the overall dispersion properties of the plasma medium. We compare the harmonics spectra from the plasma of pure tin and the plasma of tin/lead alloy. Depending on the driving wavelength, we observe enhanced harmonics at around 47 or 44 nm in both types of plasmas. The two enhanced regions could be attributed to resonances in singly-charged and doubly-charged tin ions, respectively. Our results indicate that the co-existence of lead plasma does not destroy the presence of the enhanced harmonics of tin plasma, and it seems to suggest that the macroscopic properties of the plasma are not the origin of the resonance-enhanced harmonics in tin.
Neurophysiological Correlates of Cognition as Revealed by Virtual Reality: Delving the Brain with a Synergistic Approach
Authors: Mishra Sachin , Kumar Ajay , Padmanabhan Parasuraman , Gulyás Balázs
Published: January 2021

The synergy of perceptual psychology, technology, and neuroscience can be used to comprehend how virtual reality affects cognition of human brain. Numerous studies have used neuroimaging modalities to assess the cognitive state and response of the brain with various external stimulations. The virtual reality-based devices are well known to incur visual, auditory, and haptic induced perceptions. Neurophysiological recordings together with virtual stimulations can assist in correlating humans’ physiological perception with response in the environment designed virtually. The effective combination of these two has been utilized to study human behavior, spatial navigation performance, and spatial presence, to name a few. Moreover, virtual reality-based devices can be evaluated for the neurophysiological correlates of cognition through neurophysiological recordings. Challenges exist in the integration of real-time neuronal signals with virtual reality-based devices, and enhancing the experience together with real-time feedback and control through neuronal signals. This article provides an overview of neurophysiological correlates of cognition as revealed by virtual reality experience, together with a description of perception and virtual reality-based neuromodulation, various applications, and existing challenges in this field of research.
Tractography Alterations in the Arcuate and Uncinate Fasciculi in Post-Stroke Aphasia
Authors: Kierońska Sara , Świtońska Milena , Meder Grzegorz , Piotrowska Magdalena , Sokal Paweł
Published: January 2021

Fiber tractography based on diffuse tensor imaging (DTI) can reveal three-dimensional white matter connectivity of the human brain. Tractography is a non-invasive method of visualizing cerebral white matter structures in vivo, including neural pathways surrounding the ischemic area. DTI may be useful for elucidating alterations in brain connectivity resulting from neuroplasticity after stroke. We present a case of a male patient who developed significant mixed aphasia following ischemic stroke. The patient had been treated by mechanical thrombectomy followed by an early rehabilitation, in conjunction with transcranial direct current stimulation (tDCS). DTI was used to examine the arcuate fasciculus and uncinate fasciculus upon admission and again at three months post-stroke. Results showed an improvement in the patient’s symptoms of aphasia, which was associated with changes in the volume and numbers of tracts in the uncinate fasciculus and the arcuate fasciculus.
Rho GTPases as Key Molecular Players within Intestinal Mucosa and GI Diseases
Authors: Pradhan Rashmita , Ngo Phuong A. , Martínez-Sánchez Luz d. C. , Neurath Markus F. , López-Posadas Rocío
Published: January 2021

Rho proteins operate as key regulators of the cytoskeleton, cell morphology and trafficking. Acting as molecular switches, the function of Rho GTPases is determined by guanosine triphosphate (GTP)/guanosine diphosphate (GDP) exchange and their lipidation via prenylation, allowing their binding to cellular membranes and the interaction with downstream effector proteins in close proximity to the membrane. A plethora of in vitro studies demonstrate the indispensable function of Rho proteins for cytoskeleton dynamics within different cell types. However, only in the last decades we have got access to genetically modified mouse models to decipher the intricate regulation between members of the Rho family within specific cell types in the complex in vivo situation. Translationally, alterations of the expression and/or function of Rho GTPases have been associated with several pathological conditions, such as inflammation and cancer. In the context of the GI tract, the continuous crosstalk between the host and the intestinal microbiota requires a tight regulation of the complex interaction between cellular components within the intestinal tissue. Recent studies demonstrate that Rho GTPases play important roles for the maintenance of tissue homeostasis in the gut. We will summarize the current knowledge on Rho protein function within individual cell types in the intestinal mucosa in vivo, with special focus on intestinal epithelial cells and T cells.
A New Option for the Treatment of Intrahepatic Cholangiocarcinoma: Percutaneous Hepatic Perfusion with CHEMOSAT Delivery System
Authors: Ferrucci Pier Francesco , Cocorocchio Emilia , Bonomo Guido , Varano Gianluca Maria , Della Vigna Paolo , Orsi Franco
Published: January 2021

Liver metastases are a major management problem; since they occur in tumors of different origin, they are often multiple, difficult to visualize and can lie dormant for many years. Patients with liver metastases usually die of their disease, mostly due to liver failure, since systemic treatments are unable to eradicate micro-metastasis, and interventional loco-regional procedures cannot treat all existing ones. Cholangiocarcinoma (CCA) is the second most common primary liver tumor, showing a poor overall prognosis. When resection is not possible, treatment options include tumor-focused or local ablative therapy, organ-focused or regional therapy and systemic therapy. We reviewed available loco-regional therapeutic options, with particular focus on the CHEMOSAT® Melphalan/Hepatic Delivery System (CS-HDS), which is uniquely positioned to perform a percutaneous hepatic perfusion (PHP), in order to treat the entire liver as a standalone or as complementary therapy. This system isolates the liver circulation, delivers a high concentration of chemotherapy (melphalan), filters most chemotherapy out of the blood and is a repeatable procedure. Most CS-HDS benefits are demonstrated in liver-predominant diseases, like liver metastasis from uveal melanoma (UM), hepatocarcinoma (HCC) and CCA. More than 650 procedures have been performed in Europe to date, mostly to treat liver metastases from UM. In CCA, experience is still limited, but retrospective analyses have been reported, while phase II and III studies are closed, waiting for results or ongoing.
NMDA and AMPA Receptor Autoantibodies in Brain Disorders: From Molecular Mechanisms to Clinical Features
Authors: Gardoni Fabrizio , Stanic Jennifer , Scheggia Diego , Benussi Alberto , Borroni Barbara , Di Luca Monica
Published: January 2021

The role of autoimmunity in central nervous system (CNS) disorders is rapidly expanding. In the last twenty years, different types of autoantibodies targeting subunits of ionotropic glutamate receptors have been found in a variety of patients affected by brain disorders. Several of these antibodies are directed against NMDA receptors (NMDAR), mostly in autoimmune encephalitis, whereas a growing field of research has identified antibodies against AMPA receptor (AMPAR) subunits in patients with different types of epilepsy or frontotemporal dementia. Several in vitro and in vivo studies performed in the last decade have dramatically improved our understanding of the molecular and functional effects induced by both NMDAR and AMPAR autoantibodies at the excitatory glutamatergic synapse and, consequently, their possible role in the onset of clinical symptoms. In particular, the method by which autoantibodies can modulate the localization at synapses of specific target subunits leading to functional impairments and behavioral alterations has been well addressed in animal studies. Overall, these preclinical studies have opened new avenues for the development of novel pharmacological treatments specifically targeting the synaptic activation of ionotropic glutamate receptors.
COVID-19 Emergency eLearning and Beyond: Experiences and Perspectives of University Educators
Authors: Müller Andre Matthias , Goh Charlene , Lim Li Zhen , Gao Xiaoli
Published: January 2021

Due to the COVID-19 pandemic, eLearning became the lifeline of higher education. We explored university educators’ eLearning perspectives, practices, and future adoption intentions. In-depth interviews with 14 educators from a large university in Singapore were conducted. Educators had limited eLearning experience prior to COVID-19 emergency eLearning and expressed strong preferences for in-person sessions. The short notice to switch to eLearning and lack of eLearning experiences created stress and anxiety. Educators responded by making efforts that allowed for teaching to continue, conceding that some expectations had to be readjusted. Despite many obstacles, educators acknowledged reduced apprehension towards eLearning. Reflecting upon their experiences, educators highlighted opportunities and challenges of eLearning. A key opportunity was increased flexibility, which enabled students to learn independently. Additionally, eLearning triggered reflection upon educators teaching which could lead to improved practice. Reduction of some barriers to student–educator interaction were also mentioned. Key challenges include creating social, emotional, and cognitive engagement, catering to diverse student needs and providing holistic learning experiences. Considering opportunities and challenges, educators envisioned that eLearning would feature in their future teaching if practical and helpful for achieving educational goals. Hybrid or blended learning approaches were preferred, but support enabling the implementation of technology-based and pedagogy-informed teaching is necessary.
Rumors of Nature: An Ecotranslation of Ulrike Almut Sandig’s “so habe ich sagen gehört”
Authors: Bradley Hannah
Published: January 2021

Only recently have scholars begun to discuss the implications of the Anthropocene for the translation of literature, introducing the new practice and study of ecotranslation. The Anthropocene—a term popularized by Paul Crutzen—describes the current epoch as one where human activity gains a large negative impact on geology and ecosystems. In light of this, an ecological approach to translation is not only useful but necessary for addressing the complex relationship between humans and the natural world. Ecotranslation can be understood as translation that recognizes and retains ecological themes from the source text. This study looks at the application of ecotranslation theory to an English translation of the German poem “so habe ich sagen gehört” by Ulrike Almut Sandig. The poem critiques preconceived notions about how humans relate to and conceptualize nature, making it an ideal source for applying ecotranslation. Through a close reading and interpretation of the poem, its ecological features are noted, then close attention is given to their translation. Comparison of the ecotranslation with an existing translation displays that an ecological approach can lead to a particular recognition and emphasis of ecological aspects. The resulting translation differs significantly from those translations lacking an ecological emphasis.
PACAP for Retinal Health: Model for Cellular Aging and Rescue
Authors: Pöstyéni Etelka , Kovács-Valasek Andrea , Dénes Viktória , Mester Adrienn , Sétáló György , Gábriel Róbert
Published: January 2021

Retinal aging is the result of accumulating molecular and cellular damage with a manifest decline in visual functions. Somatostatin (SST) and pituitary adenylate cyclase-activating polypeptide (PACAP) have been implicated in neuroprotection through regulating disparate aspects of neuronal activity (survival, proliferation and renewal). The aim of the present study was to validate a transgenic model for SST-expressing amacrine cells and to investigate the chronic effect of PACAP on the aging of SSTergic and dopaminergic cells of the retina. SST-tdTomato transgenic mice that were 6, 12 and 18 months old were treated intravitreally with 100 pmol of PACAP every 3 months. The density of SST and dopaminergic amacrine cells was assessed in whole-mounted retinas. Cells displaying the transgenic red fluorescence were identified as SST-immunopositive amacrine cells. By comparing the three age groups. PACAP treatment was shown to induce a moderate elevation of cell densities in both the SST and dopaminergic cell populations in the 12- and 18-month-old animals. By contrast, the control untreated and saline-treated retinas showed a minor cell loss. In conclusion, we report a reliable transgenic model for examining SSTergic amacrine cells. The fundamental novelty of this study is that PACAP could increase the cell density in matured retinal tissue, anticipating new therapeutic potential in age-related pathological processes.
Identification of Skin Lesions by Using Single-Step Multiframe Detector
Authors: Hsiao Yu-Ping , Chiu Chih-Wei , Lu Chih-Wei , Nguyen Hong Thai , Tseng Yu Sheng , Hsieh Shang-Chin , Wang Hsiang-Chen
Published: January 2021

An artificial intelligence algorithm to detect mycosis fungoides (MF), psoriasis (PSO), and atopic dermatitis (AD) is demonstrated. Results showed that 10 s was consumed by the single shot multibox detector (SSD) model to analyze 292 test images, among which 273 images were correctly detected. Verification of ground truth samples of this research come from pathological tissue slices and OCT analysis. The SSD diagnosis accuracy rate was 93%. The sensitivity values of the SSD model in diagnosing the skin lesions according to the symptoms of PSO, AD, MF, and normal were 96%, 80%, 94%, and 95%, and the corresponding precision were 96%, 86%, 98%, and 90%. The highest sensitivity rate was found in MF probably because of the spread of cancer cells in the skin and relatively large lesions of MF. Many differences were found in the accuracy between AD and the other diseases. The collected AD images were all in the elbow or arm and other joints, the area with AD was small, and the features were not obvious. Hence, the proposed SSD could be used to identify the four diseases by using skin image detection, but the diagnosis of AD was relatively poor.
Transcutaneous Electrical Acupoint Stimulation Reduces Postoperative Analgesic Requirement in Patients Undergoing Inguinal Hernia Repair: A Randomized, Placebo-Controlled Study
Authors: Szmit Mateusz , Agrawal Siddarth , Goździk Waldemar , Kübler Andrzej , Agrawal Anil , Pruchnicki Piotr , Woźniak Marta , Nowak Matylda , Bartoszewicz Bartłomiej , Rudnicki Jerzy
Published: January 2021

Given the rising rate of opioid-related adverse drug events during postsurgical pain management, a nonpharmacologic therapy that could decrease analgesic medication requirements would be of immense value. We designed a prospective, placebo-and-randomized controlled trial to assess the clinical effect of transcutaneous acupoint electrical stimulation (TEAS) on the postoperative patient-controlled analgesia (PCA) requirement for morphine, as well as side effects and recovery profile after inguinal hernia repair. Seventy-one subjects undergoing inguinal hernia repair with a standardized anesthetic technique were randomly assigned to one of three analgesic treatment regimens: PCA + TEAS (n = 24); PCA + sham-TEAS (no electrical stimulation) (n = 24), and PCA only (n = 23). The postoperative PCA requirement, pain scores, opioid-related side effects, and blood cortisol levels were recorded. TEAS treatment resulted in a twofold decrease in the analgesic requirement and decreased pain level reported by the patients. In addition, a significant reduction of cortisol level was reported in the TEAS group at 24 h postoperatively compared to the sham and control groups. We conclude that TEAS is a safe and effective option for reducing analgesic consumption and postoperative pain following inguinal hernia repair.
Dynamic Changes of Generic Quality of Life after Different Treatments for Localized Prostate Cancer
Authors: Kao Yao-Lin , Ou Chien-Hui , Lin Sheng-Hsiang , Chang Sheng-Mao , Wang Jung-Der , Tsai Yuh-Shyan
Published: January 2021

Generic quality of life (QoL) is an important issue in decision making related to the primary treatment of localized prostate cancer (PC). This study assessed the dynamic changes of QoL in patients with localized PC under different treatment modalities. From 2013 to 2018, we prospectively assessed QoL scores in patients with localized PC under unitary treatment using the World Health Organization Quality of Life (WHOQOL) BREF version. The trajectories of the QoL scores after different treatments were estimated using a kernel-smoothing method. Dynamic changes in the major determinants were analyzed using a mixed effects model. The clinical features of the participants in our institute were compared with PC patients in Taiwan’s cancer registry. A total of 196 patients were enrolled with 491 repeated assessments. The participants shared similar clinical characteristics with the PC patients in Taiwan as a whole. Patients with lower household incomes showed statistically significant lower scores on all four domains and related facets, while PC survivors with comorbidities of anxiety and/or diabetes appeared to be affected on the physical domain and related facets. After controlling for these determinants, patients under active surveillance or observation demonstrated significantly higher QoL scores in the physical and social domains, as well as several facets belonging to these domains, in mixed models compared with patients undergoing radical prostatectomy or radiotherapy within the first year. The generic QoL scores were higher within the first year in patients receiving active surveillance or observation after controlling other significant factors. The difference diminished after one year of post management. More studies are needed to corroborate our findings.
Optimal Damping Concept Implementation for Marine Vessels’ Tracking Control
Authors: Veremey Evgeny I.
Published: January 2021

This work presents the results of studies related to the design of stabilizing feedback connections for marine vessels moving along initially given trajectories. As is known, in mathematical formalization, this question leads to a problem of tracking control synthesis for nonlinear and non-autonomous plants. To provide desirable stability and performance features of the closed-loop systems to be synthesized, it is appropriate to use an optimization approach. Unlike the known synthesis methods, which are usually used within the framework of this approach, it is proposed to implement the optimal damping concept first developed by V.I. Zubov in the early 60s of the last century. Modern interpretation of this concept allows constructing numerically effective procedures of control law synthesis taking into account its applicability in a real-time regime. Central attention is focused on the questions connected with practical adaptation of the optimal damping methods for marine control systems. The operability and effectiveness of the proposed approach are illustrated by a practical example of tracking control design.
Vessel Multi-Parametric Collision Avoidance Decision Model: Fuzzy Approach
Authors: Brcko Tanja , Androjna Andrej , Srše Jure , Boć Renata
Published: January 2021

The application of fuzzy logic is an effective approach to a variety of circumstances, including solutions to maritime anti-collision problems. The article presents an upgrade of the radar navigation system, in particular, its collision avoidance planning tool, using a decision model that combines dynamic parameters into one decision—the collision avoidance course. In this paper, a multi-parametric decision model based on fuzzy logic is proposed. The model calculates course alteration in a collision avoidance situation. First, the model collects input data of the target vessel and assesses the collision risk. Using time delay, four parameters are calculated for further processing as input variables for a fuzzy inference system. Then, the fuzzy logic method is used to calculate the course alteration, which considers the vessel’s safety domain and International Regulations for Preventing Collisions at Sea (COLREGs). The special feature of the decision model is its tuning with the results of the database of correct solutions obtained with the manual radar plotting method. The validation was carried out with six selected cases simulating encounters with the target vessel in the open sea from different angles and at any visibility. The results of the case studies have shown that the decision model computes well in situations where the own vessel is in a give-way position. In addition, the model provides good results in situations when the target vessel violates COLREG rules. The collision avoidance planning tool can be automated and serve as a basis for further implementation of a model that considers the manoeuvrability of the vessels, weather conditions, and multi-vessel encounter situations.
Minimum Wage Changes across Provinces in China: Average Treatment Effects on Employment and Investment Decisions
Authors: Luo Ji , Henderson Daniel J.
Published: January 2021

We exploit data from the China Household Finance Survey to examine the impact of changes in the minimum wage on employment and investment decisions. We are able to non-parametrically identify the average treatment effect on the treated via exogenous variation in the minimum wage across provinces. We find that changes in the minimum wage had no adverse effects on employment (in terms of days worked per month or hours worked per work day) but found evidence that changes in the minimum wage impacted the percentage of families that had a bank account, a family in a rural area owned their home, and whether families (whose highest level of education was primary school) planned to purchase a home.
National Level Land-Use Changes in Functional Urban Areas in Poland, Slovakia, and Czechia
Authors: Wnęk Agnieszka , Kudas Dawid , Stych Premysl
Published: January 2021

Land-use and cover change (LUCC) impacts global environmental changes. Therefore, it is crucial to obtain cross-national level LUCC data that represents past and actual LUCC. As urban areas exhibit the most significant dynamics of the changes, accompanied by such processes as urban sprawl, it seems desirable to take into account LUCC information from such areas to acquire national level information. The paper analyses land-use changes (LUCs) in urban areas in Czechia, Poland, and Slovakia. The analysis is based on functional urban area (FUA) data from the European Urban Atlas project for 2006 and 2012. The area of urbanised land grew at the expense of agricultural areas, semi-natural areas, and wetlands over the investigated period in all three countries. The authors determined LUC direction models in urban areas based on the identified land-use change. The proposed LUC direction models for the investigated period and area should offer national level LUC data for such purposes as modelling of future changes or can be the point of reference for planning analyses. The paper proposes the following models: mean model, median model, weighted mean model where the weight is the urbanised to vegetated area ratio, and weighted mean model where the weight is the share of urbanised areas. According to the proposed LUC models, areas considered as urbanised grow in FUAs on average in six years by 5.5900‰ in Czechia, 7.5936‰ in Poland, and 4.0769‰ in Slovakia. Additionally, the change models facilitated determination of a LUC dynamics ratio in each country. It reached the highest values in Poland and the lowest in Slovakia.
Predictive Effect of Helicobacter pylori in Gastric Carcinoma Development: Systematic Review and Quantitative Evidence Synthesis
Authors: Holmes Laurens , Rios Jasmine , Berice Betyna , Benson Jacqueline , Bafford Nastocia , Parson Kadedrah , Halloran Daniel
Published: January 2021

Helicobacter pylori (H. pylori) is a bacterial pathogen implicated in gastritis, gastric ulceration, and gastric carcinoma. This study aimed to synthesize literature in providing evidence on the causative role of H. pylori in gastric carcinoma development. This study is based on assessing public literature using an applied meta-analysis, namely, quantitative evidence synthesis (QES). The analytic procedure uses DerSimonian-Laird, including assessing heterogeneity. The QES also utilizes meta-regression and the environmental effect associated with H. pylori in gastric cancer development. Eighteen studies are included in the QES. There is increased prevalence of H. pylori exposure among the cases. The heterogeneity between the CES and individual effect sizes is also significant. Despite controlling for the confoundings, there is increased exposure to H. pylori among the gastric cancer cases, regardless of the differences in the geographic location. H. pylori in this synthesized literature illustrates the contributory role of this microbe in gastric carcinoma. Additionally, regardless of geographic locale, namely, South Korea or Spain, H. pylori is implicated in gastric cancer development.
A Blue-Purple Pigment-Producing Bacterium Isolated from the Vezelka River in the City of Belgorod
Authors: Lyakhovchenko Nikita S. , Abashina Tatiana N. , Polivtseva Valentina N. , Senchenkov Vladislav Yu. , Pribylov Daniil A. , Chepurina Anna A. , Nikishin Ilja A. , Avakova Alina A. , Goyanov Michael A. , Gubina Elizaveta D. , Churikova Daria A. , Sirotin Alexander A. , Suzina Nataliya E. , Solyanikova Inna P.
Published: January 2021

Violacein is a biotechnologically significant secondary metabolite due to its antibacterial, antifungal, and other properties. Isolation, research, and identification of violacein producing strains are of interest for the development of biotechnological processes, in order to enhance the biosynthesis of this compound. The purpose of the present work was to study the properties of a newly isolated bacterium capable of synthesizing blue-purple pigment. An aboriginal bacterium was isolated from the coastal zone of the Vezelka River in the city of Belgorod. Based on chemical and spectrophotometric studies of the crude ethanol extract, the pigment was identified as violacein, and the isolate was assigned to the group of violacein-forming bacteria, which includes bacteria of the genera Chromobacterium, Iodobacter, Janthinobacterium, Duganella, Collimonas, and Massilia. Based on cultural, morphological, tinctorial, physiological, and biochemical properties, as well as analysis of the 16S rRNA gene sequence, the new isolated strain was assigned to the genus Janthinobacterium. The isolated strain is capable of suppressing the growth of a number of fungal and bacterial phytopathogens. For representatives of the genus Janthinobacterium, their inhibitory influence on cyanobacteria was shown for the first time.
The Effect of Antimicrobial Resistance Plasmids Carrying blaCMY-2 on Biofilm Formation by Escherichia coli from the Broiler Production Chain
Authors: Nesse Live L. , Mo Solveig S. , Ramstad Silje N. , Witsø Ingun L. , Sekse Camilla , Bruvoll Anna Eline E. , Urdahl Anne Margrete , Vestby Lene K.
Published: January 2021

Extended-spectrum cephalosporin-resistant Escherichia coli (ESCR E. coli) with plasmids carrying the blaCMY-2 resistance gene have been isolated from the Norwegian broiler production chain through the Norwegian monitoring program for antimicrobial resistance in animals, food and feed, NORM-VET. The aim of the present study was to investigate the biofilm forming abilities of these strains, and in particular to see whether these might be influenced by the carriage of blaCMY-2 plasmids. The ESCR E. coli from the broiler production chain displayed relatively low biofilm forming abilities in the crystal violet biofilm assay as compared to quinolone-resistant E. coli (QREC) from the same population (mean ± SD = 0.686 ± 0.686 vs. 1.439 ± 0.933, respectively). Acquisition of two different blaCMY-2 plasmids by QREC strains reduced their biofilm production in microtiter plates, but not their biofilm production on Congo Red agar plates. Furthermore, motility was reduced, but not planktonic growth. We hypothesize that genes carried by these plasmids may have caused the observed reduction in biofilm formation, possibly mediated through changes in flagellar expression or function. Furthermore, this may help explain the different biofilm forming abilities observed between ESCR E. coli and QREC. The results also indicate that the risk of biofilm reservoirs of antimicrobial resistant E. coli on in the broiler production is lower for ESCR E. coli than for QREC.
Inhibition of Type III Interferon Expression in Intestinal Epithelial Cells—A Strategy Used by Coxsackie B Virus to Evade the Host’s Innate Immune Response at the Primary Site of Infection?
Authors: Stone Virginia M. , Ringqvist Emma E. , Larsson Pär G. , Domsgen Erna , Holmlund Ulrika , Sverremark-Ekström Eva , Flodström-Tullberg Malin
Published: January 2021

Increasing evidence highlights the importance of the antiviral activities of the type III interferons (IFNλs; IL-28A, IL-28B, IL29, and IFNλ4) in the intestine. However, many viruses have developed strategies to counteract these defense mechanisms by preventing the production of IFNs. Here we use infection models, a clinical virus isolate, and several molecular biology techniques to demonstrate that both type I and III IFNs induce an antiviral state and attenuate Coxsackievirus group B (CVB) replication in human intestinal epithelial cells (IECs). While treatment of IECs with a viral mimic (poly (I:C)) induced a robust expression of both type I and III IFNs, no such up-regulation was observed after CVB infection. The blunted IFN response was paralleled by a reduction in the abundance of proteins involved in the induction of interferon gene transcription, including TIR-domain-containing adapter-inducing interferon-β (TRIF), mitochondrial antiviral-signaling protein (MAVS), and the global protein translation initiator eukaryotic translation initiation factor 4G (eIF4G). Taken together, this study highlights a potent anti-Coxsackieviral effect of both type I and III IFNs in cells located at the primary site of infection. Furthermore, we show for the first time that the production of type I and III IFNs in IECs is blocked by CVBs. These findings suggest that CVBs evade the host immune response in order to successfully infect the intestine.