bluuebunny/crossref_metadata_2025 huggingface dataset:
The Value of Marine Spatial Open Data Infrastructures—Potentials of IHO S-100 Standard tο Become the Universal Marine Data Model
Authors: Contarinis Stilianos , Pallikaris Athanasios , Nakos Byron
Published: July 2020

Marine spatial “open” data infrastructures (MSDI) have a significant economic and societal potential for coastal nations and their realization is driven by the evolution of the International Hydrographic Organization’s (IHO) S-100 data model for facilitating marine domain interoperability and the World Wide Web Consortium’s (W3C) best practices for spatial data publishing on the Web. The recent European directive on open data and the re-use of public sector information, known as the “Open Data Directive” is a key driver towards the establishment of “open” MSDIs among other spatial data infrastructures. The paper discusses possible data architectures for the MSDIs, examines the maturity of open data platforms that they could be built upon and compares the most prominent marine spatial data models for their applicability in relation to three marine information domains. MSDIs can facilitate the continuous data capturing of spatial-temporal physical phenomena and human activities at sea and coastal areas, the corresponding data analysis and the decision-making for achieving continual improvement in the marine planning and management processes. MSDIs could play a key role in digital government transformation (DGT) for effective data sharing and offering marine services across various stakeholders. The information provided through a MSDI can be used for safe and efficient operation of maritime traffic, exploration and exploitation of marine resources, marine spatial planning (MSP), integrated coastal zone management (ICZM), environmental protection, and naval and maritime security.
Experimental Validation and Comparison of Numerical Models for the Mooring System of a Floating Wave Energy Converter
Authors: Paduano Bruno , Giorgi Giuseppe , Gomes Rui P. F. , Pasta Edoardo , Henriques João C. C. , Gato Luís M. C. , Mattiazzo Giuliana
Published: July 2020

The mooring system of floating wave energy converters (WECs) has a crucial impact on power generation efficiency, cost of delivered energy, proper operation, reliability and survivability. An effective design, addressing such competing objectives, requires appropriate mathematical models to predict mooring loads and dynamic response. However, conversely to traditional offshore engineering applications, experience in modelling mooring systems for WECs is limited, due to their unique requirement of maximising the motion while minimising loads and costs. Even though modelling approaches and software are available for this application, guidelines and critical comparison are still scarce. This paper proposes a discussion and validation of three mooring-line models: one quasi-static approach (developed in-house) and two dynamic lumped-mass approaches (the open source MoorDyn and the commercial OrcaFlex). The case study is a 1:32-scale prototype of a floating oscillating water column WEC tested in a wave tank, with three mooring lines, each one comprising of a riser and a clump weight. Validation, performed by imposing fairlead displacements and comparing resulting tensions, shows good agreement. The small scale may induce numerical instabilities and uncertainties in the parameter estimation. Finally, likely due to internal resonance of this particular mooring system, high-frequency content in the mooring tension is found, albeit absent in the kinematics of the floater.
Zone of Confidence Impact on Cross Track Limit Determination in ECDIS Passage Planning
Authors: Kristić Miho , Žuškin Srđan , Brčić David , Valčić Sanjin
Published: July 2020

The technology breakthrough that Electronic Chart Display and Information System (ECDIS) has brought to modern navigation has the capability to improve the safety of navigation. This could be achieved only when the capabilities of the system are known by an end-user. Cross Track Limit (XTL) is an ECDIS safety parameter, set by the navigator, which enhances the navigational task automation in the function of workload reduction. Determination of factors affecting the value of XTL safety parameter, with special consideration to chart data reliability, is elaborated in this paper. Chart data reliability depends on the quality of chart survey data, which in many cases are outdated and unreliable. Analysis of past research on this subject is used to define the factors affecting XTL. Practices of different shipping companies with regards to XTL are analyzed and compared in order to confirm if there is a uniform practice between them. Nevertheless, shipping companies have a different or no practice of obtaining XTL, which allows the navigator to define safety parameters by a subjective opinion. In this paper, method of XTL determination for a specific vessel is suggested, considering previously defined factors. Finally, crucial influence of survey data to the safety of navigation is presented in this study.
Endangered Mediterranean Mountain Heritage—Case Study of katuns at the Kuči Mountain in Montenegro
Authors: Laković Ivan , Kapetanović Aleksandra , Pelcer-Vujačić Olga , Koprivica Tatjana
Published: July 2020

The study gives an insight into the domain of seasonal mountain settlements for summer cattle grazing (katuns), characteristic for the mountainous areas in the Mediterranean basin. The area of the Kuči Mountain in Montenegro was chosen for the case study. The area contains numerous characteristics exemplary for the topic—193 katuns with more than 2900 belonging housing and subsidiary objects. The presented results originate from the 3-year-long investigations, where the data obtained from archival documents were combined with those acquired through intensive field work and visits to each and every katun determined and documented within the area. The density of these settlements, as well as their architectural and constructional characteristics, show the high level of importance they had for the local population up until the last third of 20th century. Currently, changed sociodemographic trends rendered their intensive traditional use obsolete, but used building techniques, their internal organization and organic connection to the surrounding mountain landscape, have nominated them for important part of region’s historical heritage.
Spatial Distribution of the Micro-Mechanical Properties in High-Translucent CAD/CAM Resin-Composite Blocks
Authors: Ilie Nicoleta
Published: July 2020

Industrially cured, high-translucent computer-aided design/computer-aided manufacturing (CAD/CAM) resin-based composites (RBC) are the most recently launched dental restoratives. Clinical treatments and laboratory tests are based on a homogeneous distribution of properties within CAD/CAM blocks to obtain constant and reproducible results. The study therefore aims to determine the spatial distribution of various micro-mechanical parameters (Vickers hardness, Martens hardness, indentation modulus, creep, elastic and total indentation work) in five representative CAD/CAM RBCs. The properties of the tooth structure were evaluated under similar conditions. Filler size and shape were analyzed by scanning electron microscopy. A multivariate analysis (general linear model) identified a very strong influence of the material on all measured properties (p < 0.001; partial eta squared ηP2 > 0.943), whereby the most sensitive parameters when identifying differences within regions were the indentation modulus and the elastic indentation work. CAD/CAM RBC blocks show gradually varying properties that can increase or decrease from central to peripheral areas regardless of the chemical composition of the materials or the inorganic filler fraction. The degree of variation in the measured properties is material-specific and less than 8.7%. Clinical applications and in vitro study designs should consider slight inhomogeneity in CAD/CAM RBC blocks, while the location of the regions with best mechanical performance depends on the material.
Cu-Al2O3/Water Hybrid Nanofluid Stagnation Point Flow Past MHD Stretching/Shrinking Sheet in Presence of Homogeneous-Heterogeneous and Convective Boundary Conditions
Authors: Anuar Nur Syazana , Bachok Norfifah , Pop Ioan
Published: July 2020

The intent of this research was to present numerical solutions to homogeneous–heterogeneous reactions of the magnetohydrodynamic (MHD) stagnation point flow of a Cu-Al2O3/water hybrid nanofluid induced by a stretching or shrinking sheet with a convective boundary condition. A proper similarity variable was applied to the system of partial differential equations (PDEs) and converted into a system of ordinary (similarity) differential equations (ODEs). These equations were solved using Matlab’s in-built function (bvp4c) for various values of the governing parameters numerically. The present investigation considered the effects of homogeneous–heterogeneous reactions and magnetic field in the hybrid nanofluid flow. It was observed that dual solutions were visible for the shrinking sheet, and an analysis of stability was done to determine the physically realizable in the practice of these solutions. It was also concluded that hybrid nanofluid acts as a cooler for some increasing parameters. The magnetohydrodynamic parameter delayed the boundary layer separation; meanwhile, the nanoparticle volume fraction quickened the separation of the boundary layer that occurred. In addition, the first solution of hybrid nanofluid was found to be stable; meanwhile, the second solution was not stable. This study is therefore valuable for engineers and scientists to get acquainted with the properties of hybrid nanofluid flow, its behavior and the way to predict it.
Structures and Bioactivities of Quadrangularisosides A, A1, B, B1, B2, C, C1, D, D1–D4, and E from the Sea Cucumber Colochirus quadrangularis: The First Discovery of the Glycosides, Sulfated by C-4 of the Terminal 3-O-Methylglucose Residue. Synergetic Effect on Colony Formation of Tumor HT-29 Cells of these Glycosides with Radioactive Irradiation
Authors: Silchenko Alexandra S. , Kalinovsky Anatoly I. , Avilov Sergey A. , Andrijaschenko Pelageya V. , Popov Roman S. , Dmitrenok Pavel S. , Chingizova Ekaterina A. , Ermakova Svetlana P. , Malyarenko Olesya S. , Dautov Salim Sh. , Kalinin Vladimir I.
Published: July 2020

Thirteen new mono-, di-, and trisulfated triterpene glycosides, quadrangularisosides A–D4 (1−13) have been isolated from the sea cucumber Colochirus quadrangularis, which was collected in Vietnamese waters. The structures of these glycosides were established by 2D NMR spectroscopy and HR-ESI (High Resolution Electrospray Ionization) mass spectrometry. The novel carbohydrate moieties of quadrangularisosides D–D4 (8−12), belonging to the group D, and quadrangularisoside E (13) contain three sulfate groups, with one of them occupying an unusual position—at C(4) of terminal 3-O-methylglucose residue. Quadrangularisosides A (1) and D3 (11) as well as quadrangularisosides A1 (2) and D4 (12) are characterized by the new aglycones having 25-hydroperoxyl or 24-hydroperoxyl groups in their side chains, respectively. The cytotoxic activities of compounds 1–13 against mouse neuroblastoma Neuro 2a, normal epithelial JB-6 cells, erythrocytes, and human colorectal adenocarcinoma HT-29 cells were studied. All the compounds were rather strong hemolytics. The structural features that most affect the bioactivity of the glycosides are the presence of hydroperoxy groups in the side chains and the quantity of sulfate groups. The membranolytic activity of monosulfated quadrangularisosides of group A (1, 2) against Neuro 2a, JB-6 cells, and erythrocytes was relatively weak due to the availability of the hydroperoxyl group, whereas trisulfated quadrangularisosides D3 (11) and D4 (12) with the same aglycones as 1, 2 were the least active compounds in the series due to the combination of these two structural peculiarities. The erythrocytes were more sensitive to the action of the glycosides than Neuro 2a or JB-6 cells, but the structure–activity relationships observed for glycosides 1–13 were similar in the three cell lines investigated. The compounds 3−5, 8, and 9 effectively suppressed the cell viability of HT-29 cells. Quadrangularisosides A1 (2), C (6), C1 (7), and E (13) possessed strong inhibitory activity on colony formation in HT-29 cells. Due to the synergic effects of these glycosides (0.02 μM) and radioactive irradiation (1 Gy), a decreasing of number of colonies was detected. Glycosides 1, 3, and 9 enhanced the effect of radiation by about 30%.
Relationship between Motor Estimation Error and Physical Function in Patients with Parkinson’s Disease
Authors: Sakai Katsuya , Kawasaki Tsubasa , Ikeda Yumi , Tominaga Keita , Kurihara Kohei
Published: July 2020

Background: Motor estimation error is an index of how accurately one’s body movement is recognized. This study determines whether motor estimation error distance is a Parkinson’s disease (PD)- or age-related disability using a two-step task. Methods: The participants were 19 PD patients and 58 elderly people with disabilities. A two-step prediction test and an actual two-step test were performed. The motor estimation error distance (prediction of two-step distance minus actual two-step distance) and error rate between the two groups were compared. We conducted a correlation analysis between the motor estimation error and clinical factor (e.g., Hoehn and Yahr stage (H & Y), Unified Parkinson’s Disease Rating Scale (UPDRS)) related to PD. Results: The motor estimation error distance was not significantly different between the PD patient group and the elderly group with disabilities. However, significant correlations between motor estimation error and H & Y, and between motor estimation error and UPDRS part II, were observed. The error rate was significantly correlated with the Fall Efficacy Scale. Conclusions: The motor estimation error distance is influenced by both aging and PD.
Energy Harvesting from Brines by Reverse Electrodialysis Using Nafion Membranes
Authors: Avci Ahmet H. , Messana Diego A. , Santoro Sergio , Tufa Ramato Ashu , Curcio Efrem , Di Profio Gianluca , Fontananova Enrica
Published: July 2020

Ion exchange membranes (IEMs) have consolidated applications in energy conversion and storage systems, like fuel cells and battery separators. Moreover, in the perspective to address the global need for non-carbon-based and renewable energies, salinity-gradient power (SGP) harvesting by reverse electrodialysis (RED) is attracting significant interest in recent years. In particular, brine solutions produced in desalination plants can be used as concentrated streams in a SGP-RED stack, providing a smart solution to the problem of brine disposal. Although Nafion is probably the most prominent commercial cation exchange membrane for electrochemical applications, no study has investigated yet its potential in RED. In this work, Nafion 117 and Nafion 115 membranes were tested for NaCl and NaCl + MgCl2 solutions, in order to measure the gross power density extracted under high salinity gradient and to evaluate the effect of Mg2+ (the most abundant divalent cation in natural feeds) on the efficiency in energy conversion. Moreover, performance of commercial CMX (Neosepta) and Fuji-CEM 80050 (Fujifilm) cation exchange membranes, already widely applied for RED applications, were used as a benchmark for Nafion membranes. In addition, complementary characterization (i.e., electrochemical impedance and membrane potential test) was carried out on the membranes with the aim to evaluate the predominance of electrochemical properties in different aqueous solutions. In all tests, Nafion 117 exhibited superior performance when 0.5/4.0 M NaCl fed through 500 µm-thick compartments at a linear velocity 1.5 cm·s−1. However, the gross power density of 1.38 W·m−2 detected in the case of pure NaCl solutions decreased to 1.08 W·m−2 in the presence of magnesium chloride. In particular, the presence of magnesium resulted in a drastic effect on the electrochemical properties of Fuji-CEM-80050, while the impact on other membranes investigated was less severe.
The ComX Quorum Sensing Peptide of Bacillus subtilis Affects Biofilm Formation Negatively and Sporulation Positively
Authors: Špacapan Mihael , Danevčič Tjaša , Štefanic Polonca , Porter Michael , Stanley-Wall Nicola R. , Mandic-Mulec Ines
Published: July 2020

Quorum sensing (QS) is often required for the formation of bacterial biofilms and is a popular target of biofilm control strategies. Previous studies implicate the ComQXPA quorum sensing system of Bacillus subtilis as a promoter of biofilm formation. Here, we report that ComX signaling peptide deficient mutants form thicker and more robust pellicle biofilms that contain chains of cells. We confirm that ComX positively affects the transcriptional activity of the PepsA promoter, which controls the synthesis of the major matrix polysaccharide. In contrast, ComX negatively controls the PtapA promoter, which drives the production of TasA, a fibrous matrix protein. Overall, the biomass of the mutant biofilm lacking ComX accumulates more monosaccharide and protein content than the wild type. We conclude that this QS phenotype might be due to extended investment into growth rather than spore development. Consistent with this, the ComX deficient mutant shows a delayed activation of the pre-spore specific promoter, PspoIIQ, and a delayed, more synchronous commitment to sporulation. We conclude that ComX mediated early commitment to sporulation of the wild type slows down biofilm formation and modulates the coexistence of multiple biological states during the early stages of biofilm development.
Genomic Characterization and Environmental Distribution of a Thermophilic Anaerobe Dissulfurirhabdus thermomarina SH388T Involved in Disproportionation of Sulfur Compounds in Shallow Sea Hydrothermal Vents
Authors: Allioux Maxime , Yvenou Stéven , Slobodkina Galina , Slobodkin Alexander , Shao Zongze , Jebbar Mohamed , Alain Karine
Published: July 2020

Marine hydrothermal systems are characterized by a pronounced biogeochemical sulfur cycle with the participation of sulfur-oxidizing, sulfate-reducing and sulfur-disproportionating microorganisms. The diversity and metabolism of sulfur disproportionators are studied to a much lesser extent compared with other microbial groups. Dissulfurirhabdus thermomarina SH388T is an anaerobic thermophilic bacterium isolated from a shallow sea hydrothermal vent. D. thermomarina is an obligate chemolithoautotroph able to grow by the disproportionation of sulfite and elemental sulfur. Here, we present the results of the sequencing and analysis of the high-quality draft genome of strain SH388T. The genome consists of a one circular chromosome of 2,461,642 base pairs, has a G + C content of 71.1 mol% and 2267 protein-coding sequences. The genome analysis revealed a complete set of genes essential to CO2 fixation via the reductive acetyl-CoA (Wood-Ljungdahl) pathway and gluconeogenesis. The genome of D. thermomarina encodes a complete set of genes necessary for the dissimilatory reduction of sulfates, which are probably involved in the disproportionation of sulfur. Data on the occurrences of Dissulfurirhabdus 16S rRNA gene sequences in gene libraries and metagenome datasets showed the worldwide distribution of the members of this genus. This study expands our knowledge of the microbial contribution into carbon and sulfur cycles in the marine hydrothermal environments.
Genetic Susceptibility of the Host in Virus-Induced Diabetes
Authors: Mine Keiichiro , Yoshikai Yasunobu , Takahashi Hirokazu , Mori Hitoe , Anzai Keizo , Nagafuchi Seiho
Published: July 2020

Enteroviruses, especially Coxsackie B viruses, are among the candidate environmental factors causative of type 1 diabetes. Host genetic factors have an impact on the development of virus-induced diabetes (VID). Host background, in terms of whether the host is prone to autoimmunity, should also be considered when analyzing the role of target genes in VID. In this review, we describe the genetic susceptibility of the host based on studies in humans and VID animal models. Understanding the host genetic factors should contribute not only to revealing the mechanisms of VID development, but also in taking measures to prevent VID.
Effects of Vitamin B2 Supplementation in Broilers Microbiota and Metabolome
Authors: Biagi Elena , Mengucci Carlo , Barone Monica , Picone Gianfranco , Lucchi Alex , Celi Pietro , Litta Gilberto , Candela Marco , Manfreda Gerardo , Brigidi Patrizia , Capozzi Francesco , De Cesare Alessandra
Published: July 2020

The study of the microbiome in broiler chickens holds great promise for the development of strategies for health maintenance and performance improvement. Nutritional strategies aimed at modulating the microbiota—host relationship can improve chickens’ immunological status and metabolic fitness. Here, we present the results of a pilot trial aimed at analyzing the effects of a nutritional strategy involving vitamin B2 supplementation on the ileum, caeca and litter microbiota of Ross 308 broilers, as well as on the metabolic profile of the caecal content. Three groups of chickens were administered control diets and diets supplemented with two different dosages of vitamin B2. Ileum, caeca, and litter samples were obtained from subgroups of birds at three time points along the productive cycle. Sequencing of the 16S rRNA V3–V4 region and NMR metabolomics were used to explore microbiota composition and the concentration of metabolites of interest, including short-chain fatty acids. Vitamin B2 supplementation significantly modulated caeca microbiota, with the highest dosage being more effective in increasing the abundance of health-promoting bacterial groups, including Bifidobacterium, resulting in boosted production of butyrate, a well-known health-promoting metabolite, in the caeca environment.
Transcriptome Analysis of Testes and Uterus: Reproductive Dysfunction Induced by Toxoplasma gondii in Mice
Authors: Wang Junjie , Liu Tanghui , Mahmmod Yasser S. , Yang Zipeng , Tan Jiexing , Ren Zhaowen , Zhang Xirui , Yang Xiaoying , Zhang Xiu-Xiang , Yuan Zi-Guo
Published: July 2020

Toxoplasma gondii (T. gondii) infection in female mammals during pregnancy can result in poor pregnancy. Similarly, it can result in male reproductive disorders in male mammals. Although the testes and uterus have very different biological makeup, they are still both attacked by T. gondii resulting in reproductive dysfunctions. We hypothesized that there are significant common genes in the testes and uterus that interact with T. gondii. Finding out and studying these genes is vital to understand the infection mechanism of T. gondii and the induced disease pathogenesis. To achieve this goal, we built a mice model of acute infection with T. gondii and the testes and uterus of the mice were sequenced by RNA-Seq. A total of 291 and 679 significantly differently expressed genes (DEGs) were obtained from the testes and the uterus, respectively. In the Gene Ontology (GO) analysis, part of the DEGs in the testes and uterus were related to 35 GO functions. When compared with the KEGG database, seven pathways affecting both the testes and uterus during the course of T. gondii infection were identified. In addition, Toxoplasmosis can significantly affect the expression of Nlrp5 and Insc leading to negative outcomes in the host. On the other hand, the host regulates Gbp7, Gbp2b, and Ifit3 to defend against T. gondii infection.
Biocontrol and Plant-Growth-Promoting Traits of Talaromyces apiculatus and Clonostachys rosea Consortium against Ganoderma Basal Stem Rot Disease of Oil Palm
Authors: Goh Yit Kheng , Marzuki Nurul Fadhilah , Tuan Pa Tuan Nur Fatihah , Goh Teik-Khiang , Kee Zeng Seng , Goh You Keng , Yusof Mohd Termizi , Vujanovic Vladimir , Goh Kah Joo
Published: July 2020

Basal stem rot (BSR) disease caused by Ganoderma boninense basidiomycetous fungus is the most economically important disease in oil palms in South East Asia. Unfortunately, there is no single most effective control measure available. Tremendous efforts have been directed in incorporation of environmentally friendly biocontrol approaches in minimizing BSR disease. This study investigated the performance of two potential biocontrol agents (BCAs), AAT0115 and AAB0114 strains recovered from oil palm on suppression of BSR in planta, and also assessed their plant-growth-promoting (PGP) performance. ITS rRNA-sequence phylogeny discriminated the two ascomycetous Talaromyces apiculatus (Ta) AT0115 and Clonostachys rosea (Cr) AAB0114 biocontrol species with PGP characteristics. In vitro studies have demonstrated both Ta and Cr are capable of reducing linear mycelial growth of G. boninense. Inoculation of individual Cr and Ta—as well as Cr+Ta consortium—induced a significant increment in leaf area and bole girth of oil-palm seedlings five months post-inoculation (MPI) under nursery conditions. At five months post-inoculation, shoot and root biomass, and nutrient contents (nitrogen, phosphorus, potassium, calcium, magnesium and boron) were significantly higher in Ta-inoculated seedlings compared to control treated with non-Ta-inoculated maize. Chlorophyll and carotenoids contents in rapidly growing oil-palm seedlings challenged with Cr, Ta or a combination of both were not negatively affected. Cr, Ta and Cr+Ta consortium treated seedlings had 4.9–60% BSR disease reduction compared to the untreated control. Co-inoculation of Cr and Ta resulted in increased BSR control efficiencies by 18–26% (compared with Cr only) and 48–55% (compared with Ta only). Collectively, Cr and Ta, either individually or in consortium showed potential as BSR biocontrol agents while also possess PGP traits in oil palm.
Seasonal Variations in the Culturable Mycobiome of Acropora loripes along a Depth Gradient
Authors: Lifshitz Nofar , Hazanov Lena , Fine Maoz , Yarden Oded
Published: July 2020

Coral associated fungi are widespread, highly diverse and are part and parcel of the coral holobiont. To study how environmental conditions prevailing near the coral-host may affect fungal diversity, the culturable (isolated on potato dextrose agar) mycobiome associated with Acropora loripes colonies was seasonally sampled along a depth gradient in the Gulf of Aqaba. Fragments were sampled from both apparently healthy coral colonies as well as those exhibiting observable lesions. Based on phylogenetic analysis of 197 fungal sequences, Ascomycota were the most prevalent (91.9%). The abundance of fungi increased with increasing water depth, where corals sampled at 25 m yielded up to 70% more fungal colony forming units (CFUs) than those isolated at 6 m. Fungal diversity at 25 m was also markedly higher, with over 2-fold more fungal families represented. Diversity was also higher in lesioned coral samples, when compared to apparently healthy colonies. In winter, concurrent with water column mixing and increased levels of available nutrients, at the shallow depths, Saccharomytacea and Sporidiobolacea were more prevalent, while in spring and fall Trichocomacea (overall, the most prevalent family isolated throughout this study) were the most abundant taxa isolated at these depths as well as at deeper sampling sites. Our results highlight the dynamic nature of the culturable coral mycobiome and its sensitivity to environmental conditions and coral health.
Gut Microbiota during Dietary Restrictions: New Insights in Non-Communicable Diseases
Authors: Rinninella Emanuele , Cintoni Marco , Raoul Pauline , Ianiro Gianluca , Laterza Lucrezia , Lopetuso Loris Riccardo , Ponziani Francesca Romana , Gasbarrini Antonio , Mele Maria Cristina
Published: July 2020

In recent decades, there has been a growing interest in dietary restrictions for their promising effects on longevity and health span. Indeed, these strategies are supposed to delay the onset and burden of non-communicable diseases (NCDs) such as obesity, diabetes, cancer and neurological and gastrointestinal inflammatory diseases. At the same time, the gut microbiota has been shown to play a crucial role in NCDs since it is actively involved in maintaining gut homeostasis through its impact on nutrients metabolism, gut barrier, and immune system. There is evidence that dietary restrictions could slow down age-related changes in the types and numbers of gut bacteria, which may counteract gut dysbiosis. The beneficial effects on gut microbiota may positively influence host metabolism, gut barrier permeability, and brain functions, and subsequently, postpone the onset of NCDs prolonging the health span. These new insights could lead to the development of novel strategies for modulating gut microbiota with the end goal of treating/preventing NCDs. This review provides an overview of animal and human studies focusing on gut microbiota variations during different types of dietary restriction, in order to highlight the close relationship between gut microbiota balance and the host’s health benefits induced by these nutritional regimens.
Microbiota Changes Due to Grape Seed Extract Diet Improved Intestinal Homeostasis and Decreased Fatness in Parental Broiler Hens
Authors: Grandhaye Jeremy , Douard Veronique , Rodriguez-Mateos Ana , Xu Yifan , Cheok Alex , Riva Antonella , Guabiraba Rodrigo , Zemb Olivier , Philippe Catherine , Monnoye Magali , Staub Christophe , Venturi Eric , Barbe Alix , Ramé Christelle , Dupont Joelle , Froment Pascal
Published: July 2020

In poultry, the selection of broilers for growth performance has induced a deterioration in the health of the parental hens associated with poor reproductive efficiency. To improve these parameters, we administered to laying parental broiler hens a regular diet supplemented or not (Control) with a moderate (1%) or a high level (2%) of grape seed extract (GSE). The 1% GSE diet was administered from a young age (from 4 to 40 weeks of age) and the high level of 2% GSE was administered only during a 2-week period (from 38 to 40 weeks of age) in the laying period. The analysis of 40-week-old hens showed that 2% GSE displayed a reduction in the fat tissue and an improvement in fertility with heavier and more resistant eggs. Seven monomer phenolic metabolites of GSE were significantly measured in the plasma of the 2% GSE hens. GSE supplementation increased the relative abundance of the following bacteria populations: Bifidobacteriaceae, Lactobacilliaceae and Lachnospiraceae. In conclusion, a supplementation period of only 2 weeks with 2% GSE is sufficient to improve the metabolic and laying parameters of breeder hens through a modification in the microbiota.
A Framework on Division of Work Tasks between Humans and Robots in the Home
Authors: Saplacan Diana , Herstad Jo , Tørresen Jim , Pajalic Zada
Published: July 2020

This paper analyzes work activity in the home, e.g., cleaning, performed by two actors, a human and a robot. Nowadays, there are attempts to automate this activity through the use of robots. However, the activity of cleaning, in and of itself, is not important; it is used instrumentally to understand if and how robots can be integrated within current and future homes. The theoretical framework of the paper is based on empirical work collected as part of the Multimodal Elderly Care Systems (MECS) project. The study proposes a framework for the division of work tasks between humans and robots. The framework is anchored within existing research and our empirical findings. Swim-lane diagrams are used to visualize the tasks performed (WHAT), by each of the two actors, to ascertain the tasks’ temporality (WHEN), and their distribution and transitioning from one actor to the other (WHERE). The study presents the framework of various dimensions of work tasks, such as the types of work tasks, but also the temporality and spatiality of tasks, illustrating linear, parallel, sequential, and distributed tasks in a shared or non-shared space. The study’s contribution lies in its foundation for analyzing work tasks that robots integrated into or used in the home may generate for humans, along with their multimodal interactions. Finally, the framework can be used to visualize, plan, and design work tasks for the human and for the robot, respectively, and their work division.
Graphene-Like Layers from Carbon Black: In Vivo Toxicity Assessment
Authors: d’Amora Marta , Alfe Michela , Gargiulo Valentina , Giordani Silvia
Published: July 2020

Graphene-like (GL) layers, a new graphene-related material (GRM), possess peculiar chemical, colloidal, optical and transport properties. Considering the very recent promising application of GL layers in biomedical and bioelectronic fields, it is of utmost importance to investigate the toxicological profile of these nanomaterials. This study represents an important first report of a complete in vivo toxicity assessment of GL layers on embryonic zebrafish (Danio rerio). Our results show that GL layers do not lead to any perturbations in the different biological parameters evaluated, indicating their good biocompatibility on a vertebrate model. The new insight into the biosafety of GL layers will expand their applications in nanomedicine.
Vitamin D Synthesis Following a Single Bout of Sun Exposure in Older and Younger Men and Women
Authors: Chalcraft Jenna R. , Cardinal Linda M. , Wechsler Perry J. , Hollis Bruce W. , Gerow Kenneth G. , Alexander Brenda M. , Keith Jill F. , Larson-Meyer D. Enette
Published: July 2020

Older adults are frequently cited as an at-risk population for vitamin D deficiency that may in part be due to decreased cutaneous synthesis, a potentially important source of cholecalciferol (vitamin D3). Previous studies found that cutaneous D3 production declines with age; however, most studies have been conducted ex vivo or in the photobiology lab. The purpose of this study was to characterize the response of vitamin D metabolites following a 30-min bout of sun exposure (15-min each to the dorsal and ventral sides) at close to solar noon in younger and older adults. Methods: 30 healthy individuals with skin type II/III were recruited; a younger cohort, aged 20–37 (n = 18) and an older cohort (n = 12), age 51–69 years. Exposure was at outer limits of sensible sun exposure designed to enhance vitamin D synthesis without increasing risk of photo ageing and non-melanoma skin cancer. Serum D3 concentration was measured at baseline, 24, 48 and 72 h post-exposure. Serum 25(OH)D was measured at baseline and 72 h post-exposure plus 168 h post-exposure in the older cohort. Results: D3 increased in response to sun exposure (time effect; p = 0.002) with a trend for a difference in D3 between cohorts (time*group; p = 0.09). By regression modeling of continuous data, age accounted for 20% of the variation in D3 production. D3 production decreased by 13% per decade. Despite changes in D3, however, serum 25(OH)D did not change from baseline to 72 or 168 h post exposure (p > 0.10). Conclusions: Serum D3 concentration increased significantly in response to outdoor sun exposure in younger and older adults. While ageing may dampen cutaneous synthesis, sunlight exposure is still a significant source of vitamin D3.
Supplemental Microalgal Iron Helps Replete Blood Hemoglobin in Moderately Anemic Mice Fed a Rice-Based Diet
Authors: Bhatnagar Rohil S. , Miller Dennis D. , Padilla-Zakour Olga I. , Lei Xin Gen
Published: July 2020

Iron deficiency anemia affects 1.2 billion people globally. Our objectives were to determine if (1) supplemental iron extracted from defatted microalgae (Nannochloropsis oceanica, DGM) and (2) a combination of minute amount of plant phytase and inulin could help replete hemoglobin in anemic mice. Mice (7 weeks old) were fed a control diet (6 mg Fe/kg). After 10 weeks, the mice were assigned to three treatments: control, control + DGM iron (Fe-DGM, 39 mg Fe/kg), or control + 1% inulin + 250 units of phytase/kg (INU−PHY, 6 mg Fe/kg). The mice had free access to diets and water for 6 weeks. The Fe-DGM group had elevated blood hemoglobin (p < 0.01) and a two-fold greater (p < 0.0001) liver non-heme iron over the control. Strikingly, the INU-PHY group had 34% greater non-heme iron than the control, despite the same concentrations of iron in their diets. Fe-DGM group had altered (p < 0.05) mRNA levels of hepcidin, divalent metal transporter 1, transferrin and transferrin receptor 1. Iron extracted from defatted microalgae seemed to be effective in alleviating moderate anemia, and INU-PHY enhanced utilization of intrinsic iron present in the rice diet. Our findings may lead to a novel formulation of these ingredients to develop safer and bioavailable iron supplements for iron-deficient populations.
Vitamin-D Receptor-Gene Polymorphisms Affect Quality of Life in Patients with Autoimmune Liver Diseases
Authors: Kempinska-Podhorodecka Agnieszka , Adamowicz Monika , Chmielarz Mateusz , Janik Maciej K. , Milkiewicz Piotr , Milkiewicz Malgorzata
Published: July 2020

Vitamin D deficiency has been associated with depressive symptoms and reduced physical functioning. The aim of the study was to characterize the relationship between polymorphisms of the vitamin D receptor (VDR) gene and the quality of life in patients with autoimmune hepatitis (AIH) and primary biliary cholangitis (PBC). Three polymorphisms of the VDR gene (TaqI-rs731236, BsmI-rs1544410, and ApaI-rs7975232) were analyzed in patients with AIH (n = 142) and PBC (n = 230) and in healthy individuals (n = 376). Patient quality of life was assessed by validated questionnaires such as Medical Outcomes Study Short-Form 36 (SF-36), State Trait Anxiety Inventory (STAI), Modified Fatigue-Impact Scale (MFIS), Patient-Health Questionnaire 9 (PHQ-9), and PBC-40. The TaqI C and ApaI A alleles are risk alleles in both AIH and PBC, and a significant dominance of the A allele in BsmI was observed in AIH patients. In terms of quality of life, the presence of the CC or CT TaqI genotype was associated with emotional reactions, including the fatigue and the cognitive skills of patients with PBC, whereas in the group of AIH patients, homozygotes CC of TaqI, AA of BsmI, and AA of ApaI had worse physical, social, emotional, and mental function. The genetic variations of VDR gene can influence individual susceptibility to develop chronic autoimmune liver diseases such as AIH and PBC and affect quality of life.
Emotional Eating in Pregnant Women during the COVID-19 Pandemic and Its Association with Dietary Intake and Gestational Weight Gain
Authors: Zhang Jian , Zhang Yumei , Huo Shanshan , Ma Yidi , Ke Yalei , Wang Peiyu , Zhao Ai
Published: July 2020

Reproductive health is a significant public health issue during pandemics; however, the impacts of the novel 2019 coronavirus disease (COVID-19) on noninfected pregnant women are still unknown. This study intends (1) to examine whether emotional eating (EE) occurred during the pandemic triggered by disease concerns and (2) to explore the associations among EE, dietary changes, and gestational weight gain (GWG). Based on an online survey, 640 new mothers who experienced the lockdown in their third trimester were recruited from seven provinces in China. EE was evaluated with the Chinese version of the Dutch Eating Behavior Questionnaire, EE domain. A self-designed e-questionnaire was used to collect the data of participants on the sociodemographic characteristics, concerns about the COVID-19 pandemic, maternity information, physical activities, and dietary changes during lockdown. The results show that the average EE score was 26.5 ± 8.3, and women living in a severely affected area, who are very worried about the pandemic and who had less physical activity had a higher tendency of EE. Although there is a dietary pattern changed during pandemic, the average GWG in the studied group was in the normal range. However, a higher EE score was associated with a significant excess of GWG in women not from Wuhan (EE score 33–65 vs. 13–22: adjusted Odd Ratio (OR), 95% Confidence Interval (CI) = 1.90, 1.08–3.32). The sensitivity analysis that additionally adjusted for the pregestational body mass index and gestational metabolic disease was consistent with this result. The mediation model was also examined and showed that, after adjusting for living area and exercise, EE was associated with significantly increased consumption of cereals (EE score 33–65 vs. 13–22: adjusted OR, 95% CI = 2.22, 1.29–3.82) and oil (EE score 33–65 vs. 13–22: adjusted OR, 95% CI = 3.03, 1.06–8.69) but decreased consumption of fish and seafood (EE score 33–65 vs. 13–22: adjusted OR, 95% CI = 1.88, 1.14–3.11; 23–32 vs. 13–22: adjusted OR, 95% CI = 1.79, 1.20–2.66). In conclusion, this study indicated that EE occurred in a proportional number of pregnant women during the COVID-19 pandemic and is associated with excess GWG mediated by increased intake of certain foods. The findings suggest the need for psychosocial and nutritional education and interventions during pregnancy checkups. Further studies are needed to determine modifiable psychosocial predictors and potential nutritional concerns in pregnant women during disease outbreaks.
Flash-Like Albuminuria in Acute Kidney Injury Caused by Puumala Hantavirus Infection
Authors: Mantula Paula , Tietäväinen Johanna , Clement Jan , Niemelä Onni , Pörsti Ilkka , Vaheri Antti , Mustonen Jukka , Mäkelä Satu , Outinen Tuula
Published: July 2020

Transient proteinuria and acute kidney injury (AKI) are characteristics of Puumala virus (PUUV) infection. Albuminuria peaks around the fifth day and associates with AKI severity. To evaluate albuminuria disappearance rate, we quantified albumin excretion at different time points after the fever onset. The study included 141 consecutive patients hospitalized due to acute PUUV infection in Tampere University Hospital, Finland. Timed overnight albumin excretion (cU-Alb) was measured during the acute phase in 133 patients, once or twice during the convalescent phase within three months in 94 patients, and at six months in 36 patients. During hospitalization, 30% of the patients had moderately increased albuminuria (cU-Alb 20–200 μg/min), while 57% presented with severely increased albuminuria (cU-Alb >200 μg/min). Median cU-Alb was 311 μg/min (range 2.2–6460) ≤7 days after fever onset, 235 μg/min (range 6.8–5479) at 8–13 days and 2.8 μg/min (range 0.5–18.2) at 14–20 days. After that, only one of the measurements showed albuminuria (35.4 μg/min at day 44). At six months, the median cU-Alb was 2.0 μg/min (range 0.6–14.5). Albuminuria makes a flash-like appearance in PUUV infection and returns rapidly to normal levels within 2–3 weeks after fever onset. In the case of AKI, this is a unique phenomenon.
Peculiarities of Platelet Metabolism in Patients with Acute Coronary Syndrome with Anxiety–Depressive Disorders and Informativity of Enzymes in the Forecast of Development of Cardiovascular Complications
Authors: Shimokhina Natalya Yu. , Savchenko Andrey A. , Petrova Marina M.
Published: July 2020

Anxiety–depressive disorders (ADD) are a risk factor of cardiovascular mortality in patients with coronary artery disease (CAD). Acute coronary syndrome (ACS) is the main clinical manifestation of a progressing CAD. Metabolic processes disorder in platelets can be one of the causes of cardiovascular complications in patients with ACS and concomitant ADD. We studied platelets metabolism and prognostic informativity of NAD(P)-dependent dehydrogenases of platelets in ACS patients with ADD in terms of forecasting cardiovascular complications development over a year of observation. The levels of NAD- and NADP-dependent dehydrogenases of platelets were determined by means of a bioluminescent method during the first 24 h after admission to hospital and in dynamics in 10 days. Among 315 examined patients, ADD was found in 161 (51.1%). ACS patients with concomitant ADD had both cytoplasmic and mitochondrial processes impairment in platelets that consisted in a decrease of energy metabolism intensity, inhibition of anaerobic glycolysis reactions and lipid catabolism. After 12 months of follow-up, 41 (25.5%) cardiovascular complications were detected in the group of ACS patients with ADD and 20 (13.0%) in the group of ACS patients without ADD. According to the results of the analysis of the neural network based on NAD(P)-dependent dehydrogenases of platelets activity in ACS patients with ADD, indicators were obtained that are informative for predicting the development of recurrent cardiovascular complications.
Robotic Detection and Grasp of Maize and Sorghum: Stem Measurement with Contact
Authors: Atefi Abbas , Ge Yufeng , Pitla Santosh , Schnable James
Published: July 2020

Frequent measurements of the plant phenotypes make it possible to monitor plant status during the growing season. Stem diameter is an important proxy for overall plant biomass and health. However, the manual measurement of stem diameter in plants is time consuming, error prone, and laborious. The use of agricultural robots to automatically collect plant phenotypic data for trait measurements can overcome many of the drawbacks of manual phenotyping. The objective of this research was to develop a robotic system that can automatically detect and grasp the stem, and measure its diameter of maize and sorghum plants. The robotic system comprises of a four degree of freedom robotic manipulator, a time-of-flight camera for vision system, and a linear potentiometer sensor to measure the stem diameter. Deep learning and conventional image processing were used to detect stem in images and find grasping point of stem, respectively. An experiment was conducted in a greenhouse using maize and sorghum plants to evaluate the performance of the robotic system. The system demonstrated successful grasping of stem and a high correlation between manual and robotic measurements of diameter depicting its ability to be used as a prototype to integrate other sensors to measure different physiological and chemical attributes of the stem.
Modeling the Effect of Green Roof Systems and Photovoltaic Panels for Building Energy Savings to Mitigate Climate Change
Authors: Zheng Yuanfan , Weng Qihao
Published: July 2020

Green roofs and rooftop solar photovoltaic (PV) systems are two popular mitigation strategies to reduce the net building energy demand and ease urban heat island (UHI) effect. This research tested the potential mitigation effects of green roofs and solar photovoltaic (PV) systems on increased buildings energy demand caused by climate change in Los Angeles County, California, USA. The mitigation effects were assessed based on selected buildings that were predicted to be more vulnerable to climate change. EnergyPlus software was used to simulate hourly building energy consumption with the proper settings of PV-green roofs. All buildings with green roofs showed positive energy savings with regard to total energy and electricity. The savings caused by green roofs were positively correlated with three key parameters: Leaf Area Index (LAI), soil depth, and irrigation saturation percentage. Moreover, the majority of the electricity-saving benefits from green roofs were found in the Heating, Ventilation, and Cooling (HVAC) systems. In addition, this study found that green roofs have different energy-saving abilities on different types of buildings with different technologies, which has received little attention in previous studies.