bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of High-Quality Nuclei Isolation to Study Plant Root–Microbe Interaction for Single-Nuclei Transcriptomic Sequencing in Soybean
Authors: D’Agostino Leonidas W. , Yong-Villalobos Lenin , Herrera-Estrella Luis , Patil Gunvant B.
Published: June 2023

Single-nucleus RNA sequencing (sNucRNA-seq) is an emerging technology that has been rapidly adopted and demonstrated to be a powerful tool for detailed characterization of each cell- and sub cell-types in complex tissues of higher eukaryotes. sNucRNA-seq has also been used to dissect cell-type-specific transcriptional responses to environmental or developmental signals. In plants, this technology is being utilized to identify cell-type-specific trajectories for the study of several tissue types and important traits, including the single-cell dissection of the genetic determinants regulating plant–microbe interactions. The isolation of high-quality nuclei is one of the prerequisite steps to obtain high-quality sNucRNA-seq results. Although nuclei isolation from several plant tissues is well established, this process is highly troublesome when plant tissues are associated with beneficial or pathogenic microbes. For example, root tissues colonized with rhizobium bacteria (nodules), leaf tissue infected with bacterial or fungal pathogens, or roots infected with nematodes pose critical challenges to the isolation of high-quality nuclei and use for downstream application. Therefore, isolation of microbe-free, high-quality nuclei from plant tissues are necessary to avoid clogging or interference with the microfluidic channel (e.g., 10× Genomics) or particle-templated emulsion that are used in sNucRNA-seq platforms. Here, we developed a simple, effective, and efficient method to isolate high-quality nuclei from soybean roots and root nodules, followed by washing out bacterial contamination. This protocol has been designed to be easily implemented into any lab environment, and it can also be scaled up for use with multiple samples and applicable to a variety of samples with the presence of microbes. We validated this protocol by successfully generating a barcoded library using the 10× Genomics microfluidic platform from tissue subjected to this procedure. This workflow was developed to provide an accessible alternative to instrument-based approaches (e.g., fluorescent cell sorting) and will expand the ability of researchers to perform experiments such as sNucRNA-seq and sNucATAC-seq on inherently heterogeneous plant tissue samples.
Susceptibility of the Most Popular Soybean Cultivars in South-East Europe to Macrophomina phaseolina (Tassi) Goid
Authors: Šućur Elez Jovana , Petrović Kristina , Crnković Marina , Krsmanović Slobodan , Rajković Miloš , Kaitović Željko , Malenčić Đorđe
Published: June 2023

Oxidative stress in soybean seedlings and the length of the soybean stem lesions infected with the fungus Macrophomina phaseolina (Tassi) Goid were evaluated to determine the most tolerant soybean cultivar to this pathogen. The level of superoxide anion radical (O2•−) production, the activity of the antioxidant enzyme superoxide-dismutase (SOD), and the intensity of lipid peroxidation (LP) were measured in four soybean cultivars: Favorit, Atlas, Victoria, and Rubin. Results showed that O2•− radical production and SOD activity were the most elevated in the cv. Favorit inoculated with M. phaseolina, while the level of lipid peroxidation intensity was the lowest compared to the control. This indicates that the soybean cv. Favorit has managed to prevent infection with M. phaseolina. Furthermore, higher O2•− radical production and lower SOD enzyme activity were measured in cv. Victoria, with enhanced lipid peroxidation. This means that the cv. Victoria was infected with M. phaseolina, and was the most sensitive. None of the tested oxidative stress parameters showed a significant difference in the cvs. Atlas and Rubin compared to the control. Furthermore, the highest lesion length was measured in the cv. Victoria, followed by cv. Favorit, while the lowest lesion length was measured in the cv. Atlas followed by the cv. Rubin; and thus, the cv. Atlas followed by the cv. Rubin, were the most tolerant soybean cultivars to this pathogen.
Detrimental Effects of Induced Soil Compaction on Morphological Adaptation and Physiological Plasticity of Selected Multipurpose Tree Species
Authors: Asif Muhammad , Nawaz Muhammad Farrakh , Ahmad Irfan , Rashid Muhammad Haroon U. , Farooq Taimoor Hassan , Kashif Muhammad , Gul Sadaf , Li Qian
Published: June 2023

Soil compaction has become a global problem affecting soil worldwide. With an increased population, more demands for food and wood have resulted in intensive cultivation and increased mechanization of our farmlands and irrigated plantations. The use of heavy machinery results in soil compaction, which affects the entire soil ecosystem. This study was conducted to analyze the impact of compacted soil on germination and initial growth stages of four major agro-forest trees of central Punjab, Pakistan. Morpho-physiological traits of all selected species (Eucalyptus camaldulensis, Albizia lebbeck, Vachellia nilotica, and Zyziphus mauritiana) were measured against soil compaction. Results indicated that the root and shoot length, biomass, root–shoot ratio, diameter at root collar, no. of leaves and branches, leaf area, germination, and survival %, and physiological traits (i.e., photosynthetic rate, transpiration rate, stomatal conductance, internal CO2 concentration, and photosynthetic water use efficiency) were significantly affected by the induced soil compaction. Eucalyptus camaldulensis Dehnh. performed better and exhibited 96% germination percentage under (1.40 mg m−3) compaction level and gradually decreased by 11% with the increase of compaction level (1.80 mg m−3). It shows that the shorter roots developed due to soil compaction decreased water use efficiency, photosynthesis, and whole-plant physiological performance. The findings concluded that judicious use of machinery is highly desired for sustainable and good-quality wood production from farm trees.
The Influence of Elevated CO2 Concentrations on the Growth of Various Microalgae Strains
Authors: Chunzhuk Elizaveta A. , Grigorenko Anatoly V. , Kiseleva Sophia V. , Chernova Nadezhda I. , Ryndin Kirill G. , Kumar Vinod , Vlaskin Mikhail S.
Published: June 2023

The influence of elevated CO2 concentrations on the growth and viability of various microalgae strains was studied. Arthrospira platensis, Chlorella ellipsoidea, Chlorella vulgaris, Gloeotila pulchra, and Elliptochloris subsphaerica were tested. The cultivation of microalgae was carried out at constant CO2 concentrations (0.04, 3, 6, or 9%—sequentially from lower to higher concentrations), under constant (24 h·day−1) illumination with an intensity of 74.3 µmol quanta·m−2·s−1, and a constant temperature of 23.5 ± 0.5 °C. The optical density of the microalgae biomass, pH, and the chemical composition of the culture medium were measured. Microscopy (including the cytochemical microscopic method) was conducted to monitor the state of the microalgae. The highest biomass growth rate (0.37 g·L−1·day−1), among all experiments, was achieved for Chlorella vulgaris at CO2 = 3% and for Chlorella ellipsoidea at CO2 = 6 and 9%. The lowest growth rate (0.12 g·L−1·day−1) was achieved for Arthrospira platensis at CO2 = 3 and 9%. The microscopy results showed the absence or a minimum number of dead cells of the strains under selected conditions. The ability to maintain the viability of cultures up to significant concentrations of CO2 = 9% was due to adaptation (gradual increase in CO2 concentrations in the experiments).
Developing Functional Relationships between Soil Moisture Content and Corn Early-Season Physiology, Growth, and Development
Authors: Vennam Ranadheer Reddy , Ramamoorthy Purushothaman , Poudel Sadikshya , Reddy Kambham Raja , Henry William Brien , Bheemanahalli Raju
Published: June 2023

Drought is a severe threat to agriculture production that affects all growth stages of plants, including corn (Zea mays L.). Any factor affecting early seedling growth and development will significantly impact yield. Despite the recurrence of low rainfall during the growing seasons, corn responses to different early-season soil moisture content levels have not been investigated. In this study, we investigated how corn morpho-physiological and biomass traits responded to varied soil moisture content during the early vegetative stage. Two corn hybrids were grown in a pot-culture facility under five different soil moisture treatments (0.15, 0.12, 0.09, 0.06, and 0.03 m3 m−3 volumetric water content, VWC) to assess the growth and developmental responses to varied soil moisture content during early-season growth (V2 to V7) stage. Sub-optimal soil moisture content limited plant growth and development by reducing physiological and phenotypic expression. Stomatal conductance and transpiration were decreased by an average of 65% and 59% across stress treatments relative to optimum conditions. On average, soil moisture deficit reduced the total leaf area by 71% and 72% compared to the control in ‘A6659VT2RIB’ and ‘P1316YHR’, respectively. Shoot and root dry weights were reduced by 74% and 43% under 0.03 m3 m−3 VWC. An increase in the root-to-shoot ratio was noticed under low VWC conditions compared to the control. Based on the stress tolerance index, the physiology and leaf growth parameters were more sensitive to soil moisture deficit. Our results highlight the impact of sub-optimal soil moisture on physiology and morphological traits during early-season growth. ‘P1316YHR’ demonstrated better physiological performance under stress conditions, while ‘A6659VT2RIB’ produced relatively better root growth. The findings suggest that biomass partitioning between shoot and root components is dynamic and depends on stress intensity. The current findings can help to prioritize traits associated with the early-season drought tolerance in corn. The functional relationships developed between soil moisture content and growth and developmental responses can be integrated into corn crop modeling to allow better irrigation management decisions.
Compositional Changes in Hydroponically Cultivated Salicornia europaea at Different Growth Stages
Authors: Turcios Ariel E. , Braem Lukas , Jonard Camille , Lemans Tom , Cybulska Iwona , Papenbrock Jutta
Published: June 2023

Abiotic stress conditions, such as salinity, affect plant development and productivity and threaten the sustainability of agricultural production. Salt has been proven to accumulate in soil and water over time as a result of various anthropogenic activities and climatic changes. Species of the genus Salicornia thrive in the most saline environments and have a wide climatic tolerance. They can be found in a variety of subtropical, oceanic, and continental environments. This study aims to establish Salicornia europaea as a novel source of plant-based compounds that can grow in areas unsuitable for other crops. The morphological and compositional changes in the tissues of S. europaea in different consecutive developmental stages have not been investigated so far. Therefore, a comprehensive study of changes during the lifecycle of S. europaea was carried out, following changes in the plant’s composition, including biomass yield, and soluble and insoluble compounds. For this, plants were cultivated in hydroponics for 15 weeks and harvested weekly to analyze biomass production, to determine soluble and insoluble compounds, protein content, and polyphenols. According to the results, glucan, xylan, and lignin increase with plant age, while water extractives decrease. Protein content is higher in young plants, while flavonoid content depends on the phenological stage, decreasing in the early flowering stage and then increasing as plants enter early senescence. Our results can aid in finding the optimal harvesting stage of S. europaea, depending on the component of interest.
Cloning and Functional Analysis of the VfRR17 Gene from tung tree (Vernicia fordii)
Authors: Liao Li-Yu , He Zhang-Qi , Zhang Lin
Published: June 2023

Tung tree (Vernicia fordii) is one of the four major woody oilseed species in China. However, its fruit yield is severely hampered by the low number of female flowers and the imbalanced male-to-female flower ratio, which is a problem that restricts the development of the oilseed industry. Previous research has demonstrated that the exogenous application of cytokinins can significantly augment the number of female flowers, although the underlying regulatory mechanism remains elusive. To elucidate the involvement of VfRR17, a member of the A-type ARRs family, in the exogenous cytokinin regulation of flower sexual differentiation in tung tree, this study conducted a comprehensive bioinformatic analysis of the physicochemical properties, structural characteristics, and evolutionary relationships of the protein encoded by VfRR17. Additionally, gene function analysis was performed using subcellular localization, qRT-PCR, and genetic transformation techniques. The findings revealed that the VfRR17 gene’s coding region spanned 471 bp, encoding an unstable protein of 156 amino acids with a relative molecular mass of 17.4 kDa. Phylogenetic analysis demonstrated a higher similarity between VfRR17 of the tung tree and the RR17 gene of Jatropha curcas, Ricinus communis, Hevea brasiliensis, and other species within the Euphorbiaceae family compared to other species, with the greatest similarity of 86% observed with the RR17 gene of Jatropha curcas. The qRT-PCR analysis indicated that VfRR17 exhibited high expression levels during the early stage of tung tree inflorescence buds following 6-BA treatment, peaking at 24 h and displaying a 3.47-fold increase compared to that at 0 h. In female and male flowers of the tung tree, the expression in female flowers during the 1 DBF period was significantly higher than in male flowers, exhibiting a difference of approximately 47.91-fold. Furthermore, notable differential expression was observed in the root, leaf, and petiole segments of the tung tree under low-temperature stress at the 12-h time point. In transgenic Arabidopsis, the VfRR17 lines and wild-type lines exhibited significantly different flowering times under an exogenous 6-BA treatment at a concentration of 2 mg/L, with the VfRR17 lines experiencing an 11-day delay compared to the wild-type lines. Additionally, the number of fruit pods in VfRR17 transgenic Arabidopsis lines was significantly reduced by 28 compared to the wild-type lines at a 6-BA concentration of 3 mg/L. These findings suggest that VfRR17 likely plays a critical role in regulating flower development in response to exogenous 6-BA, providing valuable insights into the mechanisms underlying exogenous 6-BA-mediated regulation of female flower development in the tung tree.
Effects of Drought Stress on Non-Structural Carbohydrates in Different Organs of Cunninghamia lanceolata
Authors: Huang Xiaoyan , Guo Wenjuan , Yang Li , Zou Zhiguang , Zhang Xinyang , Addo-Danso Shalom Daniel , Zhou Lili , Li Shubin
Published: June 2023

The Chinese fir Cunninghamia lanceolata (Lamb.) Hook. is an important timber conifer species in China. Much has been studied about Chinese fir, but the distribution of non-structural carbohydrates (NSCs) among different organs (needles, branch, stem, and roots) under drought stress remains poorly understood. In this study, we used one-year-old C. lanceolata plantlets to evaluate the effects of simulated drought under four water regimes, i.e., adequate water or control, light drought, moderate drought, and severe drought stress corresponding to 80%, 60%, 50%, and 40%, respectively of soil field maximum capacity on various NSCs in the needles, branch, stem and roots. The degree and duration of drought stress had significant effects on fructose, glucose, sucrose, soluble sugar, starch, and NSC content in various organs (p < 0.05). Fructose content increased in stem xylem, stem phloem, and leaves. Glucose and sucrose content declined in stem and branch xylem under light drought stress and moderate drought stress, and increased under severe drought stress conditions. Soluble sugars content declined, and starch content increased in leaf and branch phloem, but the latter could not compensate for soluble sugar consumption in the whole plant, and therefore, total NSCs decreased. Correlation analysis showed that a significant positive correlation existed in the soluble sugar content between leaves and roots, and between xylem and phloem in the stems and branches. Chinese fir appears to have different NSCs distribution strategies in response to drought stress, viz., allocating more soluble sugars to fine roots and increasing starch content in the needles, as well as ensuring osmosis to prevent xylem embolism. Our study may broaden the understanding of the various mechanisms that Chinese fir and other plants have to enhance their tolerance to drought stress.
Organic Carbon, Nitrogen Accumulation and Nitrogen Leaching as Affected by Legume Crop Residues on Sandy Loam in the Eastern Baltic Region
Authors: Tripolskaja Liudmila , Kazlauskaite-Jadzevice Asta , Razukas Almantas
Published: June 2023

Legumes have a wide range of positive effects on soil properties, including nitrogen and carbon storage, soil structure and the phytosanitary condition of crops. From an agronomic point of view, legumes are most valued for their ability to take up atmospheric nitrogen in symbiosis with nitrogen-fixing bacteria. The aim of this research was to determine the effect of legume residues (peas, fodder beans, narrow-leaved lupins) on the N (Ntotal) and organic carbon (Corg) accumulation in soil and N leaching under temperate climate conditions. The experiment was carried out in lysimetric equipment in 2016–2023. The effect of legumes on Corg and Ntotal accumulation in soil and N leaching were studied in a Fabaceae–Cereals sequence. Three species of legumes—peas, fodder beans and narrow-leaved lupines—were tested; spring barley (Hordeum vulgare L.) was grown as a control treatment. The lysimeter surface area was 1.75 m2 and the experimental soil layer was 0.60 m (sand loam Haplic Luvisol). It was found that after harvesting, more residues were incorporated into the soil with lupines (p < 0.05), which, compared to pea and bean residues, increased Ntotal and Corg concentrations in the soil. There was a strong correlation (r = 0.95) between the Ntotal concentration in the soil and the N amount incorporated with residues. Mineral N released during residue decomposition was leached from the humic horizon under conditions of excess moisture in the autumn–winter period and increased the nitrate concentration in the lysimeter water. The increase in concentration was recorded within 5 to 6 months after the application of the residues. As a result, the N leaching losses increased on average by 24.7–33.2% (p < 0.05) during the year of legume cultivation. In the following year, after legume residue incorporation, the effect of residues on nitrate concentration and N leaching decreased and did not differ significantly from that of barley residues.
Biocontrol Screening of Endophytes: Applications and Limitations
Authors: Kashyap Nikhil , Singh Sandeep Kumar , Yadav Nisha , Singh Vipin Kumar , Kumari Madhuree , Kumar Dharmendra , Shukla Livleen , Kaushalendra  , Bhardwaj Nikunj , Kumar Ajay
Published: June 2023

The considerable loss of crop productivity each year due to plant disease or pathogen invasion during pre- or post-harvest storage conditions is one of the most severe challenges to achieving the goals of food security for the rising global population. Although chemical pesticides severally affect the food quality and health of consumers, a large population relies on them for plant disease management. But currently, endophytes have been considered one of the most suitable biocontrol agents due to better colonization and acclimatization potential. However, a very limited number of endophytes have been used commercially as biocontrol agents. Isolation of endophytes and their screening to represent potential characteristics as biocontrol agents are considered challenging by different procedures. Through a web search using the keywords “endophytes as biocontrol agents” or “biocontrol mechanism of endophytes,” we have succinctly summarised the isolation strategies and different in vitro and in vivo biocontrol screening methods of endophytic biocontrol agents in the present review. In this paper, biocontrol mechanisms of endophytes and their potential application in plant disease management have also been discussed. Furthermore, the registration and regulatory mechanism of the endophytic biocontrol agents are also covered.
Strategies for the Management of Aggressive Invasive Plant Species
Authors: Lorenzo Paula , Morais Maria Cristina
Published: June 2023

Current control methods for invasive alien plants (IAPs) have acceptable short-term outcomes but have proven to be unfeasible or unaffordable in the long-term or for large invaded areas. For these reasons, there is an urgent need to develop sustainable approaches to control or restrict the spread of aggressive IAPs. The use of waste derived from IAP control actions could contribute to motivating the long-term management and preservation of local biodiversity while promoting some economic returns for stakeholders. However, this strategy may raise some concerns that should be carefully addressed before its implementation. In this article, we summarize the most common methods to control IAPs, explaining their viability and limitations. We also compile the potential applications of IAP residues and discuss the risks and opportunities associated with this strategy.
Saponin Fractions from Eryngium planum L. Induce Apoptosis in Ovarian SKOV-3 Cancer Cells
Authors: Kikowska Małgorzata , Piotrowska-Kempisty Hanna , Kucińska Małgorzata , Murias Marek , Budzianowski Jaromir , Budzianowska Anna , Kaczmarek Mariusz , Kowalczyk Mariusz , Stochmal Anna , Thiem Barbara
Published: June 2023

(1) The cytotoxicity and antioxidant activity of different fractions as well as the pro-apoptotic activity of saponin fractions from Eryngium planum L. in SKOV-3 was investigated. (2) In screening studies, the cytotoxicity of six fractions on SKOV-3 was examined by LDH and SRB assays. The most active fractions—triterpenoid saponins—were selected for further investigation. To determine the mechanism of saponin fractions’ cytotoxicity, their ability to induce apoptosis was examined via Annexin V assay. The effect of the saponin fractions on caspase 3 activity was measured using a Caspase 3 Assay Kit. The expression of 84 apoptosis-related genes was investigated in cancer cells exposed to saponin fractions from the roots. The radical scavenging capacity of different fractions was determined via DPPH assay. (3) The pronounced cytotoxic effects in SKOV-3 were demonstrated by saponin fractions from the leaves and roots. Those saponin fractions were chosen for further investigation. The treatment of cancer cell lines with saponins obtained from the roots provoked a significant increase in apoptotic cells. In the SKOV-3 cells, saponins caused upregulation of pro-apoptotic genes and a decrease in anti-apoptotic genes. The activation of caspase 3 was correlated with an increased DFFA expression level in the treated SKOV-3 cells. The most active fractions were phenolic acids from the shoots and roots. (4) To the best of our knowledge, the current study is the first to demonstrate that the barrigenol-type triterpenoid saponin fraction from the roots of E. planum inhibits SKOV-3 cell proliferation and induces apoptosis, which may be regulated by the expression of genes mostly specific to a mitochondria-related pathway.
New Route of Tire Rubber Devulcanization Using Silanes
Authors: Ghosh Rounak , Mani Christian , Krafczyk Roland , Schnell Rupert , Paasche Alexander , Talma Auke , Blume Anke , Dierkes Wilma K.
Published: June 2023

The disposal of tires at the end of their lifespan results in societal and environmental issues. To tackle this, recycling and reuse are effective solutions. Among various recycling methods, devulcanization is considered to be a very sustainable option, as it involves the controlled breakdown of crosslinks while maintaining the polymer backbones. The objective of this study is to develop a sustainable devulcanization process for passenger car tire rubber using silanes. In this study, a thermo-mechanical–chemical devulcanization process was conducted to screen six potential devulcanization aids (DAs). Silanes were chosen as they are widely used in tire rubber as coupling agents for silica. The efficiency of the devulcanization was studied by the degree of network breakdown, miscibility of the devulcanized material, and mechanical properties of the de- and revulcanized material. Compared to the parent compound, a 55–60% network breakdown was achieved for the devulcanizate along with 50–55% of tensile strength recovery. In addition to superior devulcanization efficiency, this DA offers a sustainable alternative to the conventional ones, such as di-phenyl-di-sulphide, due to its compliance with safety regulations. The devulcanizate can be utilized in high-performance applications, such as tires and seals, while 100% devulcanizate can be employed in low-strength technical rubber products.
Evaluation of Organofunctionalized Polydimethylsiloxane Films for the Extraction of Furanic Compounds
Authors: Pérez-Padilla Yamile , Aguilar-Vega Manuel , Uc-Cayetano Erbin Guillermo , Esparza-Ruiz Adriana , Yam-Cervantes Marcial Alfredo , Muñoz-Rodríguez David
Published: June 2023

Hybrid membranes with three different thicknesses, PMDS_C1, PMDS_C2, and PMDS_C3 (0.21 ± 0.03 mm, 0.31 ± 0.05 mm, and 0.48 ± 0.07 mm), were synthesized by the sol–gel method using polydimethylsiloxane, hydroxy-terminated, and cyanopropyltriethoxysilane. The presence of cyano, methyl, and silicon-methyl groups was confirmed by FTIR analysis. Contact angle analysis revealed the membranes’ hydrophilic nature. Solvent resistance tests conducted under vortex and ultrasonic treatments (45 and 60 min) demonstrated a preference order of acetonitrile > methanol > water. Furthermore, the membranes exhibited stability over 48 h when exposed to different pH conditions (1, 3, 6, and 9), with negligible mass losses below 1%. The thermogravimetric analysis showed that the material was stable until 400 °C. Finally, the sorption analysis showed its capacity to detect furfural, 2-furylmethylketone, 5-methylfurfural, and 2-methyl 2-furoate. The thicker membrane was able to adsorb and slightly desorb a higher concentration of furanic compounds due to its high polarity provided by the addition of the cyano groups. The results indicated that the membranes may be suitable for sorbent materials in extracting and enriching organic compounds.
Electrospun/3D-Printed Bicomponent Scaffold Co-Loaded with a Prodrug and a Drug with Antibacterial and Immunomodulatory Properties
Authors: Cojocaru Elena , Ghitman Jana , Pircalabioru Gratiela Gradisteanu , Zaharia Anamaria , Iovu Horia , Sarbu Andrei
Published: June 2023

This work reports the construction of a bicomponent scaffold co-loaded with both a prodrug and a drug (BiFp@Ht) as an efficient platform for wound dressing, by combining the electrospinning and 3D-printing technologies. The outer component consisted of a chitosan/polyethylene oxide-electrospun membrane loaded with the indomethacin–polyethylene glycol–indomethacin prodrug (Fp) and served as a support for printing the inner component, a gelatin methacryloyl/sodium alginate hydrogel loaded with tetracycline hydrochloride (Ht). The different architectural characteristics of the electrospun and 3D-printed layers were very well highlighted in a morphological analysis performed by Scanning Electron Microscopy (SEM). In vitro release profile studies demonstrated that both Fp and Ht layers were capable to release the loaded therapeutics in a controlled and sustained manner. According to a quantitative in vitro biological assessment, the bicomponent BiFp@Ht scaffold showed a good biocompatibility and no cytotoxic effect on HeLa cell cultures, while the highest proliferation level was noted in the case of HeLa cells seeded onto an Fp nanofibrous membrane. Furthermore, the BiFp@Ht scaffold presented an excellent antimicrobial activity against the E. coli and S. aureus bacterial strains, along with promising anti-inflammatory and proangiogenic activities, proving its potential to be used for wound dressing.
Investigation of the Shear Behavior of Concrete Beams Reinforced with FRP Rebars and Stirrups Using ANN Hybridized with Genetic Algorithm
Authors: Di Bo , Qin Renyuan , Zheng Yu , Lv Jiamei
Published: June 2023

The shear strength prediction of concrete beams reinforced with FRP rebars and stirrups is one of the most complicated issues in structural engineering applications. Numerous experimental and theoretical studies have been conducted to establish a relationship between the shear capacity and the design variables. However, existing semi-empirical models fail to deliver precise predictions due to the intricate nature of shear mechanisms. To provide a more accurate and reliable model, machine learning (ML) techniques are adopted to study the shear behavior of concrete beams reinforced with FRP rebars and stirrups. A database consisting of 120 tested specimens is compiled from the reported literature. An artificial neural network (ANN) and a combination of ANN with a genetic optimization algorithm (GA-ANN) are implemented for the development of an ML model. Through neural interpretation diagrams (NID), the critical design factors, i.e., beam width and effective depth, shear span-to-depth ratio, compressive strength of concrete, FRP longitudinal reinforcement ratio, FRP shear reinforcement ratio, and elastic modulus of FRP longitudinal reinforcement rebars and FRP stirrups, are identified and determined as input parameters of the models. The accuracy of the proposed models has been verified by comparing the model predictions with the available test results. The application of the GA-ANN model provides better statistical results (mean value Vexp/Vpre equal to 0.99, R2 of 0.91, and RMSE of 22.6 kN) and outperforms CSA S806-12 predictions by improving the R2 value by 18.2% and the RMSE value by 52.5%. Furthermore, special attention is paid to the coupling effects of design parameters on shear capacity, which has not been reasonably considered in the models in the literature and available design guidelines. Finally, an ML-regression equation considering the coupling effects is developed based on the data-driven regression analysis method. The analytical results revealed that the prediction agrees with the test results with reasonable accuracy, and the model can be effectively applied in the prediction of shear capacity of concrete beams reinforced with FRP bars and stirrups.
Influence of Surface Texturing on the Dry Tribological Properties of Polymers in Medical Devices
Authors: Evangelista Isabela , Wencel Dorota , Beguin Steve , Zhang Nan , Gilchrist Michael D.
Published: June 2023

There is a constant need to improve patient comfort and product performance associated with the use of medical devices. Efforts to optimise the tribological characteristics of medical devices usually involve modifying existing devices without compromising their main design features and functionality. This article constitutes a state-of-the-art review of the influence of dry friction on polymeric components used in medical devices, including those having microscale surface features. Surface tribology and contact interactions are discussed, along with alternative forms of surface texturing. Evident gaps in the literature, and areas warranting future research are highlighted; these include friction involving polymer Vs polymer surfaces, information regarding which topologies and feature spacings provide the best performing textured surfaces, and design guidelines that would assist manufacturers to minimise or maximise friction under non-lubricated conditions.
Structure and Properties of Composite Fibers Based on Chitosan and Single-Walled Carbon Nanotubes for Peripheral Nerve Regeneration
Authors: Dresvyanina Elena N. , Tagandurdyyeva Nurjemal A. , Kodolova-Chukhontseva Vera V. , Dobrovol’skaya Irina P. , Kamalov Almaz M. , Nashchekina Yulia A. , Nashchekin Alexey V. , Ivanov Alexey G. , Yukina Galina Yu. , Yudin Vladimir E.
Published: June 2023

This study focused on a potential application of electrically conductive, biocompatible, bioresorbable fibers for tubular conduits aimed at the regeneration of peripheral nerves. The conducting, mechanical, and biological properties of composite fibers based on chitosan and single-walled carbon nanotubes were investigated in this paper. It was shown that introducing 0.5 wt.% of SWCNT into the composite fibers facilitated the formation of a denser fiber structure, resulting in improved strength (σ = 260 MPa) and elastic (E = 14 GPa) characteristics. Additionally, the composite fibers were found to be biocompatible and did not cause significant inflammation or deformation during in vivo studies. A thin layer of connective tissue formed around the fiber.
Optimization of Supercritical Carbon Dioxide Fluid Extraction of Medicinal Cannabis from Quebec
Authors: Boumghar Hinane , Sarrazin Mathieu , Banquy Xavier , Boffito Daria C. , Patience Gregory S. , Boumghar Yacine
Published: June 2023

Research on cannabis oil has evolved to encompass the pharmaceutical industry for the therapeutic potential of the active compounds for pathologies such as Alzheimer, auto-immune disorders, and cancer. These debilitating diseases are best treated with cannabinoids such as tetrahydrocannabinol (∆9-THC), cannabigerol (CBG), and cannabinol (CBN), which relieve neuropathic pain and stimulate the immune system. We extracted cannabinoids from plants with supercritical CO2 and produced an extract with a total yield close to 26%. The three-level Box–Behnken experimental design considered four factors: Temperature, pressure, CO2 flow rate, and processing time, with predetermined parameters at low, medium, and high levels. The mathematical model was evaluated by regression analysis. The yield of ∆9-THC and CBG reached a maximum after 2 h and 15 g/min of CO2, 235 bar, 55 °C (64.3 g THC/100 g of raw material and 4.6 g CBG/100 g of raw material). After another 2 h of extraction time, the yield of CBN reached 2.4 g/100 g. The regression analysis identified pressure and time as the only significant factors for total yield while pressure was the only significant factor for ∆9-THC and CBG. Time, temperature, pressure, and flow rate were all significant factors for CBN.
Laboratory Test of Fluid Physical Property Parameters of Well Fluid Containing CO2
Authors: Zou Minghua , Yu Jifei , Chen Huan , Li Menglong , Wu Guang‘ai , Shi Haowen , Bian Hanqing , Liao Xiaobo , Huang Lijuan
Published: June 2023

Change regulation of the physical properties of fluid is key to accurately predicting multiphase fluid flow in the production wellbore of CO2 flooding reservoirs. Given the characteristics of significant changes in pressure, temperature, and CO2 content in the whole wellbore of production wells in CO2 flooding reservoirs, this paper systematically studied the change rules of volume factor, viscosity, density, and solubility of well fluid for pressure 5~30 MPa, temperature 20~120 °C, and CO2 content 10~90% through single degassing PVT experiments. According to the experimental results, the volume factor of crude oil increases first and then decreases with the pressure increase. At the bubble point pressure (20 MPa), the volume factor of crude oil can reach 1.89 at high temperatures. The volume factor can be increased from 1.28 to 1.44 at 8 MPa when the temperature increases from 20 °C to 120 °C. Under the bubble point pressure, the increase in pressure increases the solubility of CO2, and the viscosity of crude oil decreases rapidly. In contrast, above the saturation pressure, the increase in pressure increases the viscosity of crude oil. Under the freezing point temperature (24 °C), the viscosity of crude oil decreases sharply with increase in temperature. In contrast, above the freezing point temperature, the viscosity change of crude oil is not sensitive to temperature. The wellbore temperature has a significant impact on the density of the well fluid. At 5 MPa, the temperature increases from 20 °C to 120 °C, which can reduce the density of high CO2 crude oil from 0.93 g/cm3 to 0.86 g/cm3. The solubility of CO2 in crude oil is sensitive to pressure. When the pressure increases from 5 MPa to 15 MPa at 20 °C, the solubility increases by 36.56 cm3/cm3. The results of this paper support the multiphase fluid flow law prediction of CO2 flooding production wells with a high gas–liquid ratio.
Finite Element Analysis and Prediction of Rock Mass Permeability Based on a Two-Dimensional Plane Discrete Fracture Model
Authors: Zhang Bochao , Wang Lixin , Liu Jianming
Published: June 2023

The safety of underground engineering projects is significantly influenced by groundwater. One of the key complexities is identifying the primary seepage paths within underground rock formations, understanding the patterns of seepage, and determining the effects of fracture parameters on the fluid movement inside the rock mass. To address these issues, a probabilistic model is constructed for random fractures using the finite element method, reflecting the random nature of fracture distributions in the real world. This model allows for an in-depth examination of the distribution of pore water pressure and Darcy velocity field, revealing the permeability trends in fractured rock masses. A variety of fracture models were devised to understand the relationship between factors such as fracture density, length, length power law, angle, dispersion coefficient, aperture, and power law, and how they affect the overall permeability of rock masses. The study suggests that, in the context of discrete fractured rock masses, there is a linear increase in permeability with an increase in fracture density and aperture. Moreover, fractures of greater length lead to increased permeability, with fractures aligned with the direction of water pressure having the most impact on seepage velocity. A thorough investigation of the factors that affect each fracture parameter was performed, and the permeability of each model was computed. From these findings, a series of predictive equations were suggested for estimating rock permeability based on fracture geometry parameters.
The Benedictine Culture of Medieval Iceland
Authors: Clark James G.
Published: June 2023

The monastic tradition of St Benedict of Nursia inspired and influenced Iceland’s medieval monasteries. Four communities, two each of men and women, which were identified in contemporary records as ‘under the rule of Saint Benedict’, endured for four hundred years, until the Protestant suppressions of the mid-sixteenth century. The monasteries of men emerged as Iceland’s most important centres of literary production; each of the churches was the focus of public worship and popular cults, and at times in their history, they may also have maintained the largest monastic populations seen in the island. With no visible trace of their physical environment, material evidence only now being revealed in excavations and very few documentary records describing their form of Benedictinism, their observant customs and broader Benedictine culture remain elusive. Drawing on the inventories (máldagar) of their property made at intervals between the thirteenth and sixteenth centuries, together with the representation of their regular life in contemporary biskupa sögur, this paper reveals a monastic practice that did diverge from that of Benedictines elsewhere in northern Europe but that nonetheless expressed a powerful attachment to some of the principal ideals of the Benedictine Rule: abbacy, conventual fraternity and the interplay of contemplative and active occupation. Above all, these communities appear to have propagated a cult interest in the figure of Benedict himself, placing him at the centre of their worship life long after Benedictines elsewhere in Europe had allowed him to be eclipsed by national and regional cults of more recent creation.
Chinese Wu, Ritualists and Shamans: An Ethnological Analysis
Authors: Winkelman Michael James
Published: June 2023

The relationship of wu (巫) to shamanism is problematic, with virtually all mentions of historical and contemporary Chinese wu ritualists translated into English as shaman. Ethnological research is presented to illustrate cross-cultural patterns of shamans and other ritualists, providing an etic framework for empirical assessments of resemblances of Chinese ritualists to shamans. This etic framework is further validated with assessments of the relationship of the features with biogenetic bases of ritual, altered states of consciousness, innate intelligences and endogenous healing processes. Key characteristics of the various types of wu and other Chinese ritualists are reviewed and compared with ethnological models of the patterns of ritualists found cross-culturally to illustrate their similarities and contrasts. These comparisons illustrate the resemblances of pre-historic and commoner wu to shamans but additionally illustrate the resemblances of most types of wu to other ritualist types, not shamans. Across Chinese history, wu underwent transformative changes into different types of ritualists, including priests, healers, mediums and sorcerers/witches. A review of contemporary reports on alleged shamans in China also illustrates that only some correspond to the characteristics of shamans found in cross-cultural research and foraging societies. The similarities of most types of wu ritualists to other types of ritualists found cross-culturally illustrate the greater accuracy of translating wu as “ritualist” or “religious ritualist.”
Observations of Ionospheric Disturbances Produced by a Powerful Very-Low-Frequency Radio Signal in the Magnetic Conjugated Region Respect the Transmitter
Authors: Feng Ting , Liu Moran , Zhou Chen
Published: June 2023

We investigate four observational cases over NWC magnetically conjugate region by DEMETER spacecraft and CSES satellite. The cases of DEMETER on 23 February 2008 and CSES on 22 March 2020 show the evident ionospheric heating effects, in which the electron density and electron temperature suggest simultaneous enhancements associated with the intense spectra of the VLF electric and magnetic field above the conjugate region. This indicates that the heating effects associated with ionospheric modification are indeed triggered by the VLF signal transmitted by the NWC transmitter. In other words, the strong disturbances induced directly above the transmitter propagate along the magnetic field lines and extend into the magnetic conjugated region. Differently, the cases on 11 February 2010 (DEMETER) and 15 February 2019 (CSES) show obvious increases in electron densities while having no significant elevation in electron temperatures. The presence of enhanced energetic electron spectra at higher L-values, rather than directly above the conjugate region, suggests precipitation events induced by the VLF transmitter.
Towards Lightweight Deep Classification for Low-Resolution Synthetic Aperture Radar (SAR) Images: An Empirical Study
Authors: Zheng Sheng , Hao Xinhong , Zhang Chaoning , Zhou Wen , Duan Lefan
Published: June 2023

Numerous works have explored deep models for the classification of high-resolution natural images. However, limited investigation has been made into a deep classification for low-resolution synthetic aperture radar (SAR) images, which is a challenging yet important task in the field of remote sensing. Existing work adopted ROC–VGG, which has a huge amount of parameters, thus limiting its application in practical deployment. It remains unclear whether the techniques developed in high-resolution natural images to make the model lightweight can be effective for low-resolution SAR images. Therefore, with prior work as the baseline, this work conducts an empirical study, testing three popular lightweight techniques: (1) channel attention module; (2) spatial attention module; (3) multi-stream head. Our empirical results show that these lightweight techniques in the high-resolution natural image domain can also be effective in the low-resolution SAR domain. We reduce the parameters from 9.2M to 0.17M while improving the performance from 94.8% to 96.8%.
A Benchmark for Multi-Modal LiDAR SLAM with Ground Truth in GNSS-Denied Environments
Authors: Sier Ha , Li Qingqing , Yu Xianjia , Peña Queralta Jorge , Zou Zhuo , Westerlund Tomi
Published: June 2023

LiDAR-based simultaneous localization and mapping (SLAM) approaches have obtained considerable success in autonomous robotic systems. This is in part owing to the high accuracy of robust SLAM algorithms and the emergence of new and lower-cost LiDAR products. This study benchmarks the current state-of-the-art LiDAR SLAM algorithms with a multi-modal LiDAR sensor setup, showcasing diverse scanning modalities (spinning and solid state) and sensing technologies, and LiDAR cameras, mounted on a mobile sensing and computing platform. We extend our previous multi-modal multi-LiDAR dataset with additional sequences and new sources of ground truth data. Specifically, we propose a new multi-modal multi-LiDAR SLAM-assisted and ICP-based sensor fusion method for generating ground truth maps. With these maps, we then match real-time point cloud data using a normal distributions transform (NDT) method to obtain the ground truth with a full six-degrees-of-freedom (DOF) pose estimation. These novel ground truth data leverage high-resolution spinning and solid-state LiDARs. We also include new open road sequences with GNSS-RTK data and additional indoor sequences with motion capture (MOCAP) ground truth, complementing the previous forest sequences with MOCAP data. We perform an analysis of the positioning accuracy achieved, comprising ten unique configurations generated by pairing five distinct LiDAR sensors with five SLAM algorithms, to critically compare and assess their respective performance characteristics. We also report the resource utilization in four different computational platforms and a total of five settings (Intel and Jetson ARM CPUs). Our experimental results show that the current state-of-the-art LiDAR SLAM algorithms perform very differently for different types of sensors. More results, code, and the dataset can be found at GitHub.
DIGITALESCAPE Project—Aerial Remote Sensing, HBIM, and Archaeology for the Preservation and Dissemination of the Cultural Heritage at Risk in the Sierra Sur and Sierra Morena Regions
Authors: Ortiz Villarejo Antonio J. , Delgado Barrado José M.
Published: June 2023

The province of Jaén (Andalusia, Spain), despite being declared the European territory with the largest number of defensive constructions (castles, fortifications, etc.), has few conservation plans, with many remains included on the Red List of Spanish Heritage lying abandoned. This poses a challenge for landscape conservation and the optimal use of the province’s tourism potential. In this paper, we present the early implementation of an ongoing methodological approach designed to alleviate this situation. It takes advantage of data from the Sentinel 2A and 2B satellites, HBIM, RPAS, GIS, and fieldwork that will be applied across five archaeological case studies. It was tested for the first time in the village of Magaña. Three main objectives and phases of this methodological approach were designed: 1. Preparation of an environmental risk chart with which to answer such questions as “How have climate change, anthropic alterations and environmental characteristics affected the state of conservation of certain heritage sites?” 2. Preparation of HBIM models to foster monitorisation and conservation policies for the main archaeological remains and improving their protection based on digital and technological tools. 3. Building a smart tourism app for mobile devices aimed at promoting smart tourism by digitalising and virtualising tourist itineraries and archaeological remains. Finally, public administrations will be apprised of the need to implement a conservation policy for cultural assets and their surroundings in a simple, quick, and cost-effective manner.