bluuebunny/crossref_metadata_2025 huggingface dataset:
Promising the potential of β-caryophyllene on mercury chloride–induced alteration in cerebellum and spinal cord of young Wistar albino rats
Authors: Yahyazadeh Ahmad , Gur Fatih Mehmet
Published: July 2024

Mercury chloride (ME) is a chemical pollutant commonly found in the environment, which can contribute to undesirable health consequence worldwide. The current study investigated the detrimental impact of ME on the cerebellum and spinal cord tissues in 6–8-week-old female rats. We also evaluated the neuroprotective efficacy of β-caryophyllene (BC) against spinal and cerebellar changes caused by ME. Thirty-five young Wistar albino rats were randomly chosen and assigned into five groups: control (CO), olive oil (OI), ME, BC, ME + BC. All samples were analysed by means of unbiased stereological, biochemical, immunohistochemical, and histopathological methods. Our biochemical findings showed that SOD level was significantly increased in the ME group compared to the CO group (p < 0.05). We additionally detected a statistically significant decrease in the number of cerebellar Purkinje cells and granular cells, as well as spinal motor neuron in the ME group compared to the CO group (p < 0.05). In the ME + BC group, the number of Purkinje cells, granular cells, and spinal motor neurons was significantly higher compared to the ME group (p < 0.05). Decreased SOD activity in the ME + BC group was also detected than the ME group (p < 0.05). Immunohistochemical (the tumour necrosis factor-alpha (TNF-α)) and histopathological examinations also exhibited crucial information in each of the group. Taken together, ME exposure was associated with neurotoxicity in the cerebellum and spinal cord tissues. BC treatment also mitigated ME-induced neurological alteration, which may imply its potential therapeutic benefits.
The positive reinforcing effects of cocaine and opposite-sex social contact: roles of biological sex and estrus
Authors: Smith Mark A. , Armas Samantha P. , Camp Jacob D. , Carlson Hannah N.
Published: July 2024

Rationale

Preclinical studies report that drug use and social contact mutually influence the reinforcing effects of one another. Most of these studies have used same-sex dyads exclusively, and the role of factors related to biological sex and hormonal fluctuations are not well understood.

Objectives

The purpose of this study was to examine the reinforcing effects of cocaine and social contact with an opposite-sex partner in male and female rats, and how these effects are modulated by ovarian hormones.

Methods

Male and female rats were trained in a nonexclusive choice procedure in which cocaine and social contact with an opposite-sex partner were simultaneously available on concurrent progressive ratio schedules of reinforcement. To examine the effects of ovarian hormones related to estrous cycling, Experiment 1 used naturally cycling, gonadally intact females, whereas Experiment 2 used ovariectomized females, and estrus was artificially induced with exogenous hormones.

Results

In both experiments, cocaine and social contact functioned as robust reinforcers, and there were no significant effects of biological sex or estrus status of the females. The positive reinforcing effects of both cocaine and social contact increased as a function of cocaine dose, indicating that contingent cocaine administration increases the reinforcing effects of social contact.

Conclusions

These data suggest that cocaine use among opposite-sex partners may enhance factors that contribute to social bonding.
Progress, applications, and challenges in high-throughput effect-directed analysis for toxicity driver identification — is it time for HT-EDA?
Authors: Alvarez-Mora Iker , Arturi Katarzyna , Béen Frederic , Buchinger Sebastian , El Mais Abd El Rahman , Gallampois Christine , Hahn Meike , Hollender Juliane , Houtman Corine , Johann Sarah , Krauss Martin , Lamoree Marja , Margalef Maria , Massei Riccardo , Brack Werner , Muz Melis
Published: July 2024

The rapid increase in the production and global use of chemicals and their mixtures has raised concerns about their potential impact on human and environmental health. With advances in analytical techniques, in particular, high-resolution mass spectrometry (HRMS), thousands of compounds and transformation products with potential adverse effects can now be detected in environmental samples. However, identifying and prioritizing the toxicity drivers among these compounds remain a significant challenge. Effect-directed analysis (EDA) emerged as an important tool to address this challenge, combining biotesting, sample fractionation, and chemical analysis to unravel toxicity drivers in complex mixtures. Traditional EDA workflows are labor-intensive and time-consuming, hindering large-scale applications. The concept of high-throughput (HT) EDA has recently gained traction as a means of accelerating these workflows. Key features of HT-EDA include the combination of microfractionation and downscaled bioassays, automation of sample preparation and biotesting, and efficient data processing workflows supported by novel computational tools. In addition to microplate-based fractionation, high-performance thin-layer chromatography (HPTLC) offers an interesting alternative to HPLC in HT-EDA. This review provides an updated perspective on the state-of-the-art in HT-EDA, and novel methods/tools that can be incorporated into HT-EDA workflows. It also discusses recent studies on HT-EDA, HT bioassays, and computational prioritization tools, along with considerations regarding HPTLC. By identifying current gaps in HT-EDA and proposing new approaches to overcome them, this review aims to bring HT-EDA a step closer to monitoring applications.

Graphical Abstract
High resolution imaging of human development: shedding light on contrast agents
Authors: Jacobs Karl , Docter Daniel , de Smit Lotte , Korfage Hans A. M. , Visser Sophie C. , Lobbezoo Frank , Hlushchuk Ruslan , de Bakker Bernadette S.
Published: July 2024

Background

Visualizing (micro)vascular structures remains challenging for researchers and clinicians due to limitations in traditional radiological imaging methods. Exploring the role of vascular development in craniofacial malformations in experimental settings can enhance understanding of these processes, with the effectiveness of high-resolution imaging techniques being crucial for successful research in this field. Micro-CT imaging offers 3D microstructural insights, but requires contrast-enhancing staining agents (CESAs) for visualizing (micro)-vascular tissues, known as contrast-enhanced micro-CT (CECT). As effective contrast agents are crucial for optimal visualization, this review focuses on comparative studies investigating such agents for micro-vascular tissue imaging using micro-CT. Furthermore, we demonstrate the utilization of B-Lugol solution as a promising contrast agent for acquiring high-quality micro-CT images of (micro)vascular structures in human embryonic samples.

Method

This scoping review followed Preferred Reporting Items for Systematic Reviews and Meta-analysis Protocols. PubMed database provided relevant articles, screened initially by title and abstract. Inclusion and exclusion criteria defined outcomes of interest.

Results

From an initial search, 273 records were identified, narrowed down to 9 articles after applying our criteria. Additionally, two articles were added through citation searching. This, a total of 11 articles were incorporated in this study.

Conclusion

This micro-CT contrast agent review underscores the need for tailored choices based on research goals. Both Barium sulfate and Iodine-based agents showing excellent results, providing high resolution (micro) vascular content, especially in ex-vivo specimens. However, careful consideration of protocols and tissue characteristics remains imperative for optimizing the effectiveness of micro-CT imaging for the study of cranio-facial vascular development.
Learning to sculpt neural cityscapes
Authors: Zhu Jialin , Wang He , Hogg David , Kelly Tom
Published: July 2024

We introduce a system that learns to sculpt 3D models of massive urban environments. The majority of humans live their lives in urban environments, using detailed virtual models for applications as diverse as virtual worlds, special effects, and urban planning. Generating such 3D models from exemplars manually is time-consuming, while 3D deep learning approaches have high memory costs. In this paper, we present a technique for training 2D neural networks to repeatedly sculpt a plane into a large-scale 3D urban environment. An initial coarse depth map is created by a GAN model, from which we refine 3D normal and depth using an image translation network regularized by a linear system. The networks are trained using real-world data to allow generative synthesis of meshes at scale. We exploit sculpting from multiple viewpoints to generate a highly detailed, concave, and water-tight 3D mesh. We show cityscapes at scales of$$100 \times 1600$$100×1600meters with more than 2 million triangles, and demonstrate that our results are objectively and subjectively similar to our exemplars.
Comparison and clinical analysis of antibiotics and endoscopic injection for vesicoureteral reflux in children
Authors: Yang Zhen , Dai Kanglin , Sun Xianglun , Tian Chen , Yuan Lihua , Liu Jingsi , Li Ban , Chung Patrick Ho Yu , Wong Kenneth Kak Yuen
Published: July 2024

Purpose

This study evaluated the outcome of pediatric patients with primary vesicoureteral reflux (VUR) and compared of the treatments between continued antibiotic prophylaxis (CAP) and endoscopic injection.

Methods

The clinical data of children diagnosed with primary vesicoureteral reflux from March 2015 to June 2020 who were treated with antibiotics or endoscopic injection were reviewed. Antibiotic was the first-chosen treatment after the diagnosis of VUR in children. Endoscopic treatment consisted of injection of dextran hyaluronic acid copolymer (DX/HA) into the ureteral opening under direct cystoscopy guidance.

Results

Fifty-two children (35 males, 17 females) were included in this study, and for a total 90 ureters (14 unilateral, 38 bilateral) were diagnosed with vesicoureteral reflux by Voiding cystourethrography (VCUG). Twenty-two children were treated with antibiotics (8 unilateral, 14 bilateral), for a total of 36 ureters; thirty children were treated by endoscopic injection (6 unilateral, 24 bilateral), for a total of 54 ureters. The injection surgery took 36 ± 17 min including duration of general anesthesia and circumcision and the hospital stay was 2.3 ± 1.3 days. All male patients underwent circumcision simultaneously. There were no drug and allergic reactions in the antibiotic group, and no postoperative complications occurred in the injection group. With 23 months (13–63 months) of mean follow-up, the resolution rate, defined as radiological disappearance of VUR, was 36.1% (13/36) in the antibiotic group and 57.4% (31/54) in the injection group (P = 0.048).Two cases of bilateral reflux in the injection group required a second injection before resolution could be achieved. Thus, the overall success rate of injection was 64.8% (35/54). 9 cases (9/18, 50%) in the antibiotic group had renal scars on DMSA scans, while this was seen in 20 cases (20/23, 86.9%) in the injection group. There was a statistically significant difference between the two groups (P = 0.010).The positive rates of ultrasound between the antibiotic group and the injection group were 45.5% (10/22) and 80.0% (24/30), respectively. There was a statistically significant difference between the two groups in positive rates of ultrasound (P = 0.010).

Conclusions

Endoscopic injection is easy to operate with short surgical time and hospital stay, so it is a safe and feasible treatment. For the treatment of primary vesicoureteral reflux in children, the radiological resolution rate of endoscopic injection is better than antibiotic therapy. In this study, the presence of kidney scars on DMSA and the dilated of the collecting system on ultrasound are the indications for endoscopic injection.
Protective defunctioning stoma in bowel segmental resection at the time of total hysterectomy for endometriosis: when less is more
Authors: Dolci Carolina , Kerbage Yohan , Ruffolo Alessandro Ferdinando , Candiani Massimo , Gandon Anne , Rubod Chrystèle
Published: July 2024

Purpose

To compare postoperative complications in women undergoing total hysterectomy with segmental resection (TH-SR) for intestinal endometriosis with or without protective defunctioning stoma (PDS) confection.

Methods

Retrospective cohort study conducted at the Gynecologic department of University Hospital of Lille (France) from January 2008 to January 2022 in patients undergone TH-SR for bowel endometriosis.

Results

100 women were considered for the analysis. PDS were performed in 56 women. The rate of rectal resections was significantly higher in the PDS group (p = 0.03). The mean operative time, AAGL scores and length of hospital stay were significantly higher in the PDS group (p = 0.002). The rate of grade III complication according to Clavien-Dindo classification was higher in the PDS group (p = 0.03). Among digestive complications, one case of anastomosis leakage (1.8%) and one case of recto-vaginal fistula (2.3%) was recorded in the non-PDS group, 4 cases of anastomosis stenosis were recorded in the PDS group (7.1%). Persisting bladder atony requiring self-catheterization over one month was the most common disturb (4.6% in the non-PDS group and 7.1% in the PDS group, p = 0.58). The distance of digestive lesion from anal margin was the only risk factor for digestive complications, persistent bladder atony, Clavien-Dindo IIIA and IIIB complications at the multivariate analysis (p = 0.04 and p = 0.06 respectively).

Conclusion

No statistically significant differences were found in the rate of digestive complications in case of total hysterectomy and concomitant segmental resection when performing or not preventing stoma.
Cellobionate production from sodium hydroxide pretreated wheat straw by engineered Neurospora crassa HL10
Authors: Wang Jiajie , Kasuga Takao , Fan Zhiliang
Published: July 2024

This study investigated cellobionate production from a lignocellulosic substrate using Neurospora crassa HL10. Utilizing NaOH-pretreated wheat straw as the substrate obviated the need for an exogenous redox mediator addition, as lignin contained in the pretreated wheat served as a natural mediator. The low laccase production by N. crassa HL10 on pretreated wheat straw caused slow cellobionate production, and exogenous laccase addition accelerated the process. Cycloheximide induced substantial laccase production in N. crassa HL10, enabling the strain to yield approximately 57 mM cellobionate from pretreated wheat straw (equivalent to 20 g/L cellulose), shortening the conversion time from 8 to 6 days. About 92% of the cellulose contained in the pretreated wheat straw is converted to cellobionate. In contrast to existing methods requiring pure cellobiose or cellulase enzymes, this process efficiently converts a low-cost feedstock into cellobionate at a high yield without enzyme or redox mediator supplementation.
A discontinuous Galerkin/cohesive zone model approach for the computational modeling of fracture in geometrically exact slender beams
Authors: Kota Sai Kubair , Kumar Siddhant , Giovanardi Bianca
Published: July 2024

Slender beams are often employed as constituents in engineering materials and structures. Prior experiments on lattices of slender beams have highlighted their complex failure response, where the interplay between buckling and fracture plays a critical role. In this paper, we introduce a novel computational approach for modeling fracture in slender beams subjected to large deformations. We adopt a state-of-the-art geometrically exact Kirchhoff beam formulation to describe the finite deformations of beams in three-dimensions. We develop a discontinuous Galerkin finite element discretization of the beam governing equations, incorporating discontinuities in the position and tangent degrees of freedom at the inter-element boundaries of the finite elements. Before fracture initiation, we enforce compatibility of nodal positions and tangents weakly, via the exchange of variationally-consistent forces and moments at the interfaces between adjacent elements. At the onset of fracture, these forces and moments transition to cohesive laws modeling interface failure. We conduct a series of numerical tests to verify our computational framework against a set of benchmarks and we demonstrate its ability to capture the tensile and bending fracture modes in beams exhibiting large deformations. Finally, we present the validation of our framework against fracture experiments of dry spaghetti rods subjected to sudden relaxation of curvature.
External validation of a clinical mathematical model estimating post-operative urine output following cardiac surgery in children
Authors: Baloglu Orkun , Marino Bradley S. , Latifi Samir Q. , Morca Ayse , Munther Daniel S. , Ryan Shawn D.
Published: July 2024

Background

This study aims to externally validate a clinical mathematical model designed to predict urine output (UOP) during the initial post-operative period in pediatric patients who underwent cardiac surgery with cardiopulmonary bypass (CPB).

Methods

Children aged 0–18 years admitted to the pediatric cardiac intensive care unit at Cleveland Clinic Children’s from April 2018 to April 2023, who underwent cardiac surgery with CPB were included. Patients were excluded if they had pre-operative kidney failure requiring kidney replacement therapy (KRT), re-operation or extracorporeal membrane oxygenation or KRT requirement within the first 32 post-operative hours or had indwelling urinary catheter for fewer than the initial 32 post-operative hours, or had vasoactive-inotrope score of 0, or those with missing data in the electronic health records.

Results

A total of 213 encounters were analyzed; median age (days): 172 (IQR 25–75th%: 51–1655), weight (kg): 6.1 (IQR 25–75th%: 3.8–15.5), median UOP ml/kg/hr in the first 32 post-operative hours: 2.59 (IQR 25–75th%: 1.93–3.26) and post-operative 30-day mortality: 1, (0.4%). The mathematical model achieved the following metrics in the entire dataset: mean absolute error (95th% Confidence Interval (CI)): 0.70 (0.67–0.73), median absolute error (95th% CI): 0.54 (0.52–0.56), mean squared error (95th% CI): 0.97 (0.89–1.05), root mean squared error (95th% CI): 0.99 (0.95–1.03) and R2 Score (95th% CI): 0.29 (0.24–0.34).

Conclusions

This study provides encouraging external validation results of a mathematical model predicting post-operative UOP in pediatric cardiac surgery patients. Further multicenter studies must explore its broader applicability.

Graphical abstract
Optimization of the different controller parameters via OBL approaches based artificial ecosystem optimization involving fitness distance balance guiding mechanism for efficient motor speed regulation of DC motor
Authors: Isen Evren , Duman Serhat
Published: July 2024

This study proposes a new optimization approach, which is called as artificial ecosystem optimization algorithm with fitness-distance balance guiding mechanism by using opposite based learning methods (FDBAEO_OBLs) for the speed regulation of direct current (DC) motor. The performance of the proposed FDBAEO_OBL algorithm is tested in two different experimental studies. In the first experimental study, the proposed approach is tested in the CEC2020 benchmark test functions and the FDBAEO algorithm, which included the best OBL approach, is determined using non-parametric Wilcoxon and Friedman statistical analysis methods. Second, the parameters of proportional integral derivative (PID), tilt integral derivative (TID), proportional integral derivative with filter (PIDF), tilt integral derivative with filter (TIDF), fractional-order proportional integral derivative (FOPID), fractional-order proportional integral derivative with filter (FOPIDF), proportional integral derivative with fractional-order filter (PIDFF) and fractional-order proportional integral derivative with fractional-order filter (FOPIDFF) controller structures to be used in DC motor closed loop speed control are determined with FDBAEO_OBL, and the performances of the controllers are investigated. Integral absolute error (IAE), integral time absolute error (ITAE), integral time squared error (ITSE) and integral squared error (ISE) performance indices are used as the objective function of the operation process in which the control parameters are determined. According to the comparative step response results of the controller structures, the four best controller structures for DC motor speed regulation are determined. The performances of these controllers are examined under different simulation conditions and according to the results obtained, it is seen that the best controller structure is FOPIDFF. The FDBAEO_OBL algorithm, which is used in both benchmark test functions and DC motor speed regulation, shows an effective, durable and superior performance in finding the optimal solution values during the optimization.
Subnational assessment of legal and illegal deforestation in the Colombian Amazon: consequences for zero deforestation commitments
Authors: Katz-Asprilla David , Piketty Marie-Gabrielle , Briceño Castillo Guido , Blanc Lilian , Camacho Peña Jhon , Karsenty Alain
Published: July 2024

Deforestation has been at the heart of the transformation of the Amazon. Global concerns over deforestation and its impact on climate change have resulted in the adoption of a number of initiatives in the framework of zero deforestation. However, undifferentiated application of the concepts of zero net, gross, and illegal deforestation has revealed a lack of understanding of their scope and of challenges to their implementation. Zero legal and illegal deforestation is, in particular, a controversial subject from the point of view of regulation and sovereignty but an essential aspect from the perspective of public policy design. In Colombia, zero deforestation commitments make no mention of legal deforestation. Papers that analyze deforestation and official data sources fail to incorporate the legal dimension in their analysis. This article addresses this gap by identifying areas where deforestation is legal and where it is illegal in one deforestation hotspot of the Colombian Amazon, the administrative department of Guaviare. Our results show that deforestation has increased since 2013, mainly occurs in illegal zones, and that Guaviare department, 85% of which is covered by forest, has very little legal deforestation potential. Our findings reveal that assessing the legality and illegality of deforestation is more complex than often assumed and must become a priority, especially in forested countries where regulation on forest and land management is shared between different level of governments and institutions.
A global-scale applicable framework of landslide dam formation susceptibility
Authors: Wu Hang , Trigg Mark A. , Murphy William , Fuentes Raul , Martino Salvatore , Esposito Carlo , Marmoni Gian Marco , Scarascia Mugnozza Gabriele
Published: July 2024

The formation and failure of landslide dams is an important and understudied, multi-hazard topic. A framework of landslide dam formation susceptibility evaluation was designed for large-scale studies to avoid the traditional dependence on landslide volume calculations based on empirical relationships, which requires comprehensive local inventories of landslides and landslide dams. The framework combines logistic regression landslide susceptibility models and global fluvial datasets and was tested in Italy and Japan based on landslide and landslide dam inventories collected globally. The final landslide dam formation susceptibility index identifies which river reach is most prone to landslide dam formation, based on the river width and the landslide susceptibility in the adjacent delineated slope drainage areas. The logistic regression models showed good performances with area under the receiver operating characteristics curve values of 0.89 in Italy and 0.74 in Japan. The index effectively identifies the probability of landslide dam formation for specific river reaches, as demonstrated by the higher index values for river reaches with past landslide dam records. The framework is designed to be applied globally or for other large-scale study regions, especially for less studied data-scarce regions. It also provides a preliminary evaluation result for smaller catchments and has the potential to be applied at a more detailed scale with local datasets.
Valuing farmers in transitions to more sustainable food systems: A systematic literature review of local food producers’ experiences and contributions in short food supply chains
Authors: O’Connor Grace , Reis Kimberley , Desha Cheryl , Burkett Ingrid
Published: July 2024

Industrial food systems are being increasingly challenged by alternative food movements globally that advocate for better environmental, social, economic, and political outcomes as part of societal transitions to more sustainable food systems. At the heart of these transitions are local food producers operating within shorter food supply chains, their experiences, and their knowledge of ecologically sustainable food production, biodiversity and climate, and their communities. Despite their important contributions to the resilience of food systems, society and ecology, local food producers' experiences and knowledges are often undervalued, ignored, or inaccurately reflected. This systematic literature review identifies the values, motivations, and concerns as key elements of the experiences of local food producers within short food supply chains across literature globally, their contributions to social-ecological resilience, and discusses how these experiences and contributions can influence transitions to sustainable food systems. Eighty-five research articles were distilled from 5 databases and thematic analysis revealed four major themes: (1) concerns for exploitative operating contexts and hidden labor expectations within short food supple chains, (2) local food producers’ value and need for social networks, (3) their environmental values, connections, and concerns, and (4) how they can value and be motivated by alternative models but are concerned by their economic viability. This review also observed an important paradox within local food producers’ experiences showing that whilst farming is a demanding profession and lifestyle, they can feel a deep fulfilment when they live and work in harmony with their values and motivations. This systematic review is significant for how it values and synthesizes the experiences of local food producers and the diverse personal, social, ecological, and economic contributions that local food production has for social-ecological resilience of communities. By better communicating these experiences and contributions to decision-makers, policy makers and planners, this research can have major implications for enabling societal transitions that are fairer and more just in ways that empower, protect, and privilege local food producers’ voices.
To Improve Motivational Barriers to Retention in High Resolution Anoscopy, Patients and Providers Recommend Social and Environmental Changes: A Sequential Explanatory Mixed-Methods Pilot Study in a Federally Qualified Health Center
Authors: Kutner Bryan A. , Hou Baichun , Giguere Rebecca , DeWitt Will , Tan Mei , Rael Christine Tagliaferri , Radix Asa , Sandfort Theodorus G. M.
Published: July 2024

Loss to follow-up (LTFU) in high-resolution anoscopy (HRA) programs jeopardizes the procedure’s potential to help prevent anal cancer. We explored quality improvement factors to understand how to address this LTFU. Using the transtheoretical COM-B Model (Capability, Opportunity, Motivation, and Behavior) and a sequential explanatory mixed-methods design, we surveyed and interviewed 13 patients who remained engaged in HIV care but who delayed their HRA monitoring or treatment visits in the same community clinic, and 6 HRA clinicians and medical assistants. Analyses involved descriptive statistics and rapid qualitative analysis. Patients were racially, ethnically, and economically representative of the LTFU population, and were generally experienced with HRA (Mean HRA visits = 4.6, SD = 2.8, mdn = 3). Providers were experienced clinicians and medical assistants (Mean years providing HRA = 6.0, SD = 2.2). Analyses revealed two primary, related barriers: (A) motivational barriers such as physical pain, discomfort, embarrassment, and anxiety; which were largely borne from (B) opportunity barriers such as difficulties with scheduling, inconsistent after-care (particularly for pain and discomfort), anxiety-inducing exam rooms and equipment, and internalized and anticipated stigma. Capability barriers, such as limited health literacy about HRA, were less common and, like motivational barriers, linked to opportunity barriers. Participants recommended potential facilitators, including easier scheduling, standardization of pain management and after-care services, and examination room modifications to reduce anxiety. To retain HRA patients in community settings, interventions should address social and physical opportunity barriers that strongly determine motivational and capability barriers. Improving convenience, standardizing pain management, and introducing stigma interventions specific to HRA, could alleviate both motivational and capability barriers.
Positivity and convexity in incomplete cooperative games
Authors: Černý Martin , Bok Jan , Hartman David , Hladík Milan
Published: July 2024

Incomplete cooperative games generalize the classical model of cooperative games by omitting the values of some of the coalitions. This allows for incorporating uncertainty into the model and studying the underlying games and possible payoff distributions based only on the partial information. In this paper, we conduct a systematic investigation of incomplete games, focusing on two important classes: positive and convex games. Regarding positivity, we generalize previous results from a special class of minimal incomplete games to a general setting. We characterize the non-extendability to a positive game by the existence of a certificate and provide a description of the set of positive extensions using its extreme games. These results also enable the construction of explicit formulas for several classes of incomplete games with special structures. The second part deals with convexity. We begin with the case of non-negative, minimal incomplete games. We establish the connection between incomplete games and the problem of completing partial functions and, consequently, provide a characterization of extendability and a full description of the set of symmetric convex extensions. This set serves as an approximation of the set of convex extensions.
Rethinking AI code generation: a one-shot correction approach based on user feedback
Authors: Le Kim Tuyen , Andrzejak Artur
Published: July 2024

Code generation has become an integral feature of modern IDEs, gathering significant attention. Notable approaches like GitHub Copilot and TabNine have been proposed to tackle this task. However, these tools may shift code writing tasks towards code reviewing, which involves modification from users. Despite the advantages of user feedback, their responses remain transient and lack persistence across interaction sessions. This is attributed to the inherent characteristics of generative AI models, which require explicit re-training for new data integration. Additionally, the non-deterministic and unpredictable nature of AI-powered models limits thorough examination of their unforeseen behaviors. We propose a methodology named One-shot Correction to mitigate these issues in natural language to code translation models with no additional re-training. We utilize decomposition techniques to break down code translation into sub-problems. The final code is constructed using code snippets of each query chunk, extracted from user feedback or selectively generated from a generative model. Our evaluation indicates comparable or improved performance compared to other models. Moreover, the methodology offers straightforward and interpretable approaches, which enable in-depth examination of unexpected results and facilitate insights for potential enhancements. We also illustrate that user feedback can substantially improve code translation models without re-training. Ultimately, we develop a preliminary GUI application to demonstrate the utility of our methodology in simplifying customization and assessment of suggested code for users.
Identifying remnant biodiversity hotspots in Southern Asia reveals disequilibrium in mammalian communities
Authors: Sartor Caroline Charão , Kaszta Zaneta , Kamler Jan , Hearn Andrew J. , Ash Eric , Bolongon Gilmoore , Can Özgün Emre , Channa Phan , Cheyne Susan , Fitzmaurice Amy , Haidir Iding Achmad , Kyaw Pyae Phyoe , Luskin Matthew Scott , Singh Priya , Rasphone Akchousanh , Wei Cedric Tan Kai , Yadav Bhupendra Prasad , Cushman Samuel A. , Macdonald David W.
Published: July 2024

Identification of highly biodiverse areas has become a crucial step in protecting species richness, especially considering the rapid collapse of biodiversity and the limited funds available to avert, far less to reverse, these trends. Therefore, we aimed to identify the most important areas for the conservation of specified mammalian groups in Southern Asia, a region rich in biodiversity hotspots threatened by increasing rates of habitat loss and other anthropogenic activities. To achieve this, we modelled the occupancy of ungulates and of small, medium and large carnivorans at 20 study sites across the region and identified hotspots of species richness. We analysed the variation of estimated space use between different species groups and ranked areas according to their predicted importance for mammalian species conservation. Our results reveal a significant positive correlation in the spatial utilization patterns of competitive carnivores, yet no correlation among carnivores and their prey species, suggesting that anthropogenic impacts in the region are constraining species to coexist in only the few remaining suitable areas, superseding interactions between species guilds. Although the rank of site importance varied amongst species groups, we were able to identify a consensus on sites that are crucial for the conservation of all groups considered. Most of these top-ranking sites were located in the peninsular region of Thailand. We argue that, of the areas assessed, these sites represent the most important refuges for species conservation in the region, and their protection is critical for the maintenance of the biodiversity in Southern Asia.
Microbial hydrogenation of cholesterol to coprostanol by anaerobic bacteria: evidence from Antarctic lacustrine sediment
Authors: Chen Xin , Wang Jianjun , Jin Jing , Nie Yaguang , Zheng Zhangqin , Xue Yulu , Kong Weidong , Cheng Wenhan , Zhang Jifeng , Liang Lewen , Yang Yi , Emslie Steven D. , Liu Xiaodong
Published: July 2024

Fecal sterols are traditionally ascribed as important biomarkers for animal excrement, and have been widely used to identify the source of organic matter and to reconstruct paleoecological changes in Antarctic terrestrial, aquatic, and marine ecosystems. However, the in situ microbial hydrogenation of cholesterol to coprostanol could have significance as a proxy to infer paleoenvironmental studies in Antarctica, particularly in anoxic sediment. Here, we report that abundant coprostanol, which was traditionally deemed as a biomarker for human sewage contamination, was found in three anoxic sediment profiles (AC2, BI, and CH1), which were strongly influenced by animal excrement at North Victoria Land, western Ross Sea, Antarctica. Our results suggest that the high concentrations of coprostanol in these three sediment profiles were not due to animal excrement, since coprostanol is not present in penguin guano and is a minor component in seal excrement. Coprostanol/cholesterol and coprostanol/(coprostanol + cholestanol) ratios suggest that coprostanol in the sediment cores of AC2 and BI was primarily derived from bacterial hydrogenation of cholesterol introduced by penguin guano. Coprostanol in CH1 sediments is related to human sewage due to intensive research activities from 1968 to 2006 in this region. However, the low abundance of coprostanol and the ratios of coprostanol/cholesterol and coprostanol/(coprostanol + cholestanol) in a relatively oxidizing sediment core (IIL1) infer that coprostanol was likely contributed by seal settlement. Together with high-throughput sequencing of the 16S rRNA gene, the conversion of cholesterol to coprostanol by anaerobic bacteria (e.g., Eubacterium coprostanoligenes) could occur in anoxic aquatic systems. Our results suggest that the presence of coprostanol in Antarctic lacustrine sediment with anoxic conditions does not necessarily indicate seal activity and human waste as the microbial hydrogenation of cholesterol to coprostanol should also be considered.
Biotic regulation of nitrogen gas emissions in temperate agriculture
Authors: Almaraz Maya , Ryals Rebecca , Groffman Peter , Porder Stephen
Published: July 2024

It is generally assumed that fertilizer addition is the prime driver of nitrogen (N) gas loss from modern cropping systems. This assumption has its basis in observations of nitrous oxide (N2O, an important greenhouse gas) emissions, and is contrary to theory from unmanaged ecosystems, where N losses are controlled by plant physiological influence on the soil environment. However, dinitrogen (N2) emissions are likely a major N loss pathway in both managed and unmanaged ecosystems, but these emissions are very difficult to measure. We directly measured N2 and N2O emissions from two temperate agricultural systems over the course of the growing season to test when total N gas losses are highest. We hypothesized that N2 emissions mirror those of N2O, with the largest flux immediately after fertilization, early in the growing season. Instead, we found that N2 emissions were highest at the end of the growing season, and were most strongly correlated with soil moisture, which increased after plant senescence. Dinitrogen emissions were an order of magnitude larger than N2O. Thus, while N2O emissions were highest following fertilization, overall N gas loss was greatest at the end of the growing season. These data suggest that total N gas losses are high and have different temporal patterns from N2O fluxes. Understanding the magnitude and controls over these losses are important for understanding and managing the N cycle of temperate agricultural systems.
Socially Irresponsible HRM: Findings from the UK Hotel Sector
Authors: Walker Victoria , Nickson Dennis
Published: July 2024

This paper considers the value and extent to which socially responsible HRM enhances understanding of HR practices in the corporate hotel sector. The paper seeks to address two research questions. Firstly, what are the underlying management philosophies guiding models of HRM within the upper market corporate hotel sector? Secondly, how do the resultant HR practices impact the employee experience of work and well-being? Qualitative case studies were conducted in two high end hotels within the UK. Semi structured interviews (n = 30) were carried out at various levels of the organisation to gain multiple perspectives, including frontline employees, line managers, senior management and HR practitioners. Investigation of the experiences of frontline employees uncovered evidence of a socially irresponsible approach to HRM in each case study. Hidden and deceptive management philosophies were uncovered that shaped the nature of the HR practices used, and resulted in negative outcomes for the well-being of employees. This paper extends the limited research base which has considered socially irresponsible HRM, and extends the concept by demonstrating the central role that management philosophy plays in determining the responsibleness of an HRM approach. The paper also demonstrates the utility of SRHRM models in contexts where practices are rarely socially responsible.
Striated muscle: an inadequate soil for cancers
Authors: Saunders Alastair A. E. , Thomson Rachel E. , Goodman Craig A. , Anderson Robin L. , Gregorevic Paul
Published: July 2024

Many organs of the body are susceptible to cancer development. However, striated muscles—which include skeletal and cardiac muscles—are rarely the sites of primary cancers. Most deaths from cancer arise due to complications associated with the development of secondary metastatic tumours, for which there are few effective therapies. However, as with primary cancers, the establishment of metastatic tumours in striated muscle accounts for a disproportionately small fraction of secondary tumours, relative to the proportion of body composition. Examining why primary and metastatic cancers are comparatively rare in striated muscle presents an opportunity to better understand mechanisms that can influence cancer cell biology. To gain insights into the incidence and distribution of muscle metastases, this review presents a definitive summary of the 210 case studies of metastasis in muscle published since 2010. To examine why metastases rarely form in muscles, this review considers the mechanisms currently proposed to render muscle an inhospitable environment for cancers. The “seed and soil” hypothesis proposes that tissues’ differences in susceptibility to metastatic colonization are due to differing host microenvironments that promote or suppress metastatic growth to varying degrees. As such, the “soil” within muscle may not be conducive to cancer growth. Gaining a greater understanding of the mechanisms that underpin the resistance of muscles to cancer may provide new insights into mechanisms of tumour growth and progression, and offer opportunities to leverage insights into the development of interventions with the potential to inhibit metastasis in susceptible tissues.
Comparing the Mixed Logit Estimates and True Parameters under Informative and Uninformative Heterogeneity: A Simulated Discrete Choice Experiment
Authors: Jumamyradov Maksat , Craig Benjamin M. , Greene William H. , Munkin Murat
Published: July 2024

In discrete choice experiments (DCEs), differences between respondents’ preferences may be associated with observable or unobservable factors. Unobservable heterogeneity, related to latent factors associated with the choices of individuals, may be modelled using correlated (i.e. informative heterogeneity) or uncorrelated (i.e. uninformative heterogeneity) individual-specific parameters of a logit model. In this study, we simulated unobservable heterogeneity among DCE respondents and compared the results of the maximum simulated likelihood (MSL) estimation of the mixed logit model when correctly specified and mis-specified. These results show that the MSL estimates are biased and can differ greatly from the true parameters, even when correctly specified. Before estimating a mixed logit model, we highly recommend that choice modellers conduct simulation analyses to assess the potential extent of biases before relying on the MSL estimates, particularly their variances and correlations, and then ultimately determine which model specification produces the least bias.
Exploring the influence of rapeseed cultivar and pathogen isolate on Acremonium alternatum's efficacy in clubroot disease control
Authors: Auer Susann , Zamani-Noor Nazanin , Mahfoud Yamen , Ludwig-Müller Jutta
Published: July 2024

Clubroot disease, caused by Plasmodiophora brassicae, ranks among the most significant diseases affecting rapeseed cultivars, leading to substantial annual yield losses. Current control methods are limited to a small selection of chemical or biological treatments. Using biocontrol organisms presents a promising strategy for reducing disease severity and promoting plant vigour. However, their efficacy is strongly dependent on biotic and abiotic factors during the growing season, as well as the specific application conditions. In the present study, we evaluated the efficacy of the biocontrol fungus Acremonium alternatum in reducing clubroot disease symptoms across different susceptible and resistant rapeseed cultivars (Brassica napus) under various experimental greenhouse settings employing different types of P. brassicae inoculum: a uniform single spore isolate e3 and two German field isolates P1 and P1 ( +). We found that A. alternatum can reduce clubroot disease symptoms in susceptible rapeseed cultivars Visby, Ability and Jenifer, but not cv. Jumbo, when inoculated with the aggressive single spore isolate P. brassicae e3 at moderate (106 spores mL−1) and high (107 spores mL−1) densities. A. alternatum enhanced plant vitality and shoot biomass in cv. Visby inoculated with field isolates P1 or P1 ( +) but did not considerably reduce clubroot severity there. The clubroot-resistant cv. Mentor displayed a reduction in clubroot symptoms after A. alternatum treatment. In conclusion, A. alternatum holds some promise in managing moderate P. brassicae levels in the soil and could serve as an option in integrated pest management of clubroot disease when combined with resistant cultivars.
Instrumental Needs: A Relational Account
Authors: Stabell Espen Dyrnes
Published: July 2024

Instrumentalism about need suggests that the normative significance of an agent’s need for x depends on the end for which x is needed. Instrumental accounts have, however, been vague about the transfer or transmission of normative significance supposed to be occurring from ends to needs. How should such transmission be understood, and how can we assess the amount or degree of significance being transmitted in particular cases? The Relational Account (RA) combines work on normative transmission principles and the strength of reasons in order to clarify these issues. RA, it is argued, both (1) improves the instrumental view on need and (2) can be used to analyze and assess a large range of needs and arguments from need – including ‘basic needs’, which some argue require non-instrumental explanation. While the paper develops an instrumental view, the analysis of the normativity of the needs-end relation will also be helpful for clarifying instrumental relations between different kinds of need in non-instrumentalist theories – such as relations between ‘absolute’ and intermediate needs.
Local impedance drop–guided versus lesion size index–guided pulmonary vein isolation
Authors: Lian Evgeny , Pantlik Robert , Maslova Vera , Willert Sven , Moser Fabian , Remppis Andrew , Frank Derk , Demming Thomas
Published: July 2024

Background

Local tissue impedance drop (LID) and lesion size index (LSI) technologies are valuable for predicting effective lesion formation. This study compares the acute and long-term efficacy of LID-guided versus LSI-guided pulmonary vein isolation (PVI) for atrial fibrillation treatment.

Methods

We retrospectively analyzed two patient groups undergoing radiofrequency PVI. In the LID-guided group (n = 35), ablation was performed without contact force monitoring, stopping at the LID plateau (target LID 12 Ohm posterior, 16 Ohm anterior). In the LSI-guided group (n = 31), ablation used contact force information with target LSI (5 anterior, 4 posterior). Both groups utilized a power of 40 W anterior and 30 W posterior, with < 6 mm inter-lesion distance. Gap mapping and touch-up ablation were done if necessary.

Results

PVI was achieved with a significantly shorter ablation time in the LSI-guided group (25 min [21;31] vs 30 [27;35], p = 0.035). PV gaps were more frequent in the LID-guided group (74% vs 42%, p = 0.016). Over 11.5 ± 2.9 months follow-up, arrhythmia recurrence was higher in the LID-guided group (34.3% vs 16.1%, p = 0.037). A redo procedure performed in 10 (28.6%) patients in the LID-guided group and 3 (9.7%) in the LSI-guided group showed chronic PV reconnections in 7 out of 10 (70%) and 2 out of 3 (67%) patients, respectively.

Conclusions

LSI-guided ablation results in shorter ablation time and fewer PV gaps compared to LID-guided ablation. Despite initial success, LID-guided ablation had higher arrhythmia recurrence and PV reconnections during long-term follow-up compared to LSI-guided ablation.

Graphical abstract