bluuebunny/crossref_metadata_2025 huggingface dataset:
Perbandingan Pelaksanaan Pelayanan Kolaborasi Masa Nifas terhadap Kejadian Postpartum Blues di Puskesmas Sewon I & Banguntapan II Kabupaten Bantul Tahun 2020
Authors: Hertati Dessy , Nurdiati Detty Siti , Sulistyaningsih Sulistyaningsih , Dasuki Djaswadi
Published: May 2022

Postpartum blues is a mild mood disorder syndrome often ignored by postpartum mothers, families, or health workers. Postpartum blues can develop into depression and even psychosis. The incidence varies significantly in Asia, between 26-85%, whereas the incidence is around 50-70% in Indonesia. Postpartum blues also affect the interaction between the baby and the mother, especially during the first year. In mental health, collaborative practice can increase patient and health team satisfaction, reduce the duration of treatment, reduce the cost of care, reduce the incidence of suicide, and reduce outpatient visits. The study aimed to analyze the differences between the implementation of postpartum collaboration services and postpartum blues at Sewon I & Banguntapan II Primary Health Center of Bantul Regency in 2019. This study applied a comparative study with a prospective cohort approach, and the sampling technique used consecutive sampling. Data analysis employed the Wilcoxon test, Mann-Whitney, and binary regression. Wilcoxon test p=0,000 means a difference in the initial and final EPDS score reduction between the two Primary Health Centers and the postpartum blues incidence. The number of postpartum blues in Sewon I Primary Health Center reached 52 (44.83%), while in Banguntapan II, there were 45 (42.45%). Fisher exact test results pregnancy relationship p = 0.642 and history of depression p = 0.078 meaning that there was no relationship. The binary regression test of the age obtained p = 0.000 and parity p = 0.007, meaning a relationship. It can be concluded that Inter-professional Collaboration has become an effective and efficient strategy to improve the quality of services and patient health outcomes due to the increasing diversity of professions in the health sector and the increasingly complex patient problems.
Hubungan Motivasi terhadap Perilaku Pemberantasan Sarang Nyamuk Demam Berdarah Dengue (DBD)
Authors: Yupita Yupita , Frisilia Melisa , Indriani Indriani
Published: May 2022

Dengue hemorrhagic fever (DHF) is one of the public health problems in Indonesia because the number of sufferers and the area of distribution is increasing along with increased mobility and population density. The motivations and behavior of the still diverse people often hinder the PSN movement. In addition, it is also supported by not maximal actions or activities for eradicating mosquito nests (PSN) in the community, causing extraordinary events (KLB). The targets are mosquito breeding places, such as water reservoirs for daily needs or natural water reservoirs or yards. To determine the relationship of motivation to the behavior of eradicating dengue mosquito nests and using the literature review method. The research uses an academic database, namely Google Scholar, published in 2017-2020 using Indonesian and English. Study design Cross-sectional. They obtained six research results according to the inclusion criteria. The research sample is an average of 40-109 respondents. The results of the research analysis showed six research journals that there was a relationship between the factors that influenced the administration of HB-0 immunization in newborns with a value (? = 0.05). There is a relationship between knowledge, family support, occupation, age, education, and motivation in the factors influencing the administration of HB-0 immunization in newborns.
Studi Pola Penggunaan Obat Anti Tuberkulosis pada Pasien Tuberkulosis Paru
Authors: Naftali Andrea Verencia , Al Farizi Gilang Rizki , Ovikariani Ovikariani
Published: May 2022

Tuberculosis is one infectious disease that is still a significant problem in the health sector. This study aims to determine the pattern of anti-tuberculosis drugs in pulmonary tuberculosis patients, including the right dose, right patient, right time of administration, right route of administration, and alert for side effects based on the 2017 National Guidelines for Tuberculosis Control. The method used is descriptive with data collection systematically. Retrospectively in the form of medical record data for 2019 to 2020 at the Semarang Barat District Health Center, Semarang City, namely as many as 35 pulmonary tuberculosis patients. The study of drug side effects was traced based on the literature from several journals. The results of the univariate analysis showed that the use of anti-pulmonary tuberculosis drugs was obtained 100% at the right dose, 100% by the right patient, 100% at the time of administration, and 100% correct method of administration. Meanwhile, the side effects of using fixed-dose combination category 1 anti-tuberculosis drugs based on the literature are nausea, vomiting, redness of the urine, fever, lack of appetite, itching, and skin redness. The use of drugs in pulmonary tuberculosis patients at the Semarang Barat District Health Center, Semarang City, is in by the 2017 National Guidelines for Tuberculosis Control, a reference for the appropriate use of anti-tuberculosis drugs.
ПСИХОКОНСУЛЬТАТИВНА ВЗАЄМОДІЯ З ДЕЗАДАПТОВАНИМИ ПІДЛІТКАМИ У СИТУАЦІЇ РОЗЛУЧЕННЯ БАТЬКІВ
Authors: Герасіна С. , Хомич Г.
Published: May 2022

У статті актуалізовано проблему порушення психосоціальної адаптації підлітків в умовах розлучення батьків. Розкрито вікові особливості і кризові тенденції формування підлітка. Досліджено психосоціальну дезадаптацію підлітків у комунікаціях з рідними, ровесниками і педагогами, у звичних їхніх справах і заняттях. Запропоновано психологічний супровід підлітка на засадах особистісно орієнтованого консультування у руслі тріадної взаємодії: «дитина – родина – школа».
РОЛЬ ЕМОЦІЙНОГО ІНТЕЛЕКТА В ОСОБИСТІСНІЙ РЕГУЛЯЦІЇ РІШЕНЬ І ДІЙ КЕРІВНИКА ЯК ЛІДЕРА
Authors: Гриньова Н.
Published: May 2022

Загальнопсихологічне дослідження місця та ролі емоційного інтелекту у системах регуляції прийняття рішень передбачає звернення до вибірок осіб професій, у яких він необхідно включений у діяльність та спілкування. Емоційний інтелект і толерантність до невизначеності можна розглядати як властивості, що сприяють ефективним та результативним рішенням.
ПСИХОЛОГІЧНІ ОСОБЛИВОСТІ КОПІНГ-ПОВЕДІНКИ ЯК ФОРМИ ПОДОЛАННЯ СТРЕСУ У САМОТНІХ ЖІНОК В УМОВАХ НЕВИЗНАЧЕНОСТІ
Authors: Ніколаєв Л. , Руденко О. , Чижма Д. , Озеллі О.
Published: May 2022

У статті презентовано результати теоретичного аналізу та емпіричного дослідження психологічних особливостей прояву копінг-поведінки у самотніх жінок, як форми подолання стресу в умовах невизначеності. Визначено місце копінг-стратегій у збереженні психічного здоров’я в умовах стресу. Проаналізовано планування вирішення проблеми, самоконтроль, пошук соціальної підтримки та конфронтацію як переважаючі копінг-стратегії досліджуваних.
Two Letters of Marsilio Ficino and Their Translations in Humanistic Bohemia
Authors: Nejeschleba Tomáš
Published: May 2022

Two of Marsilio Ficino’s letters among his correspondence—the letter De officiis and the letter Veritas de institutione principis—enjoyed heightened popularity in the sixteenth century. One of the first translations of Ficino into the vernacular is a translation of these two letters into Czech, printed in Prague around the year 1500. Czech humanist Řehoř Hrubý of Jelení (ca. 1460–1514) seems to be a plausible candidate for the authorship. The second translation into Czech was published in 1520 by Oldřich Velenský of Mnichov (1495–1531). This article examines the Czech humanistic translations of Ficino’s letters and places them into the context of the Czech humanistic movement at the begin­ning of the sixteenth century, which emphasized moral topics from Italian Renaissance Platonism. It argues that these letters from Ficino supported the moral claims of Czech pre-Reformation and Reformation thought at the beginning of the sixteenth century.
The Senses of Sight in Lancelot Andrewes’s Sermons
Authors: Norris Paul
Published: May 2022

Lancelot Andrewes constructed visually intense scenes and symbolic images to capture his auditors’ interest, but he never let them forget that his visual enargia was rooted in the words of scripture and had no reality outside that given by God’s Word. His strategy was, therefore, not only to make Christ’s presence concrete and accessible to his listeners, but also to hint at how God will always be out of reach, a truth that cannot be seen. In exploring this rhetorical strategy, I focus especially on Andrewes’s 1604 Good Friday sermon, 1620 Easter sermon, and 1620 consecration rite. The two sermons were delivered at opposite ends of Andrewes’s career in James’s court, but both are notable for their intense scrutiny of what it means to see Christ in the eyes of the mind, body, and soul.
Perancangan Sistem Informasi Akademik Berbasis Web di SMK YABP 1 Garut
Authors: Setiawan Ridwan , Agustin Yoga Handoko , Putuwenda Ditdit , Ramdani Dendi
Published: May 2022

Sekolah Menengah Kejuruan (SMK) YABP 1 Garut merupakan lembaga pendidikan yang di mana pada saat ini kegiatan operasional pembelajaran, baik dalam administrasi, absensi, dan penilaian menggunakan sistem manual dalam kertas dan buku-buku induk data sehingga membutuhkan waktu yang cukup lama untuk melakukan kegiatan-kegiatan perekapan ataupun pengelolaannya sehingga dalam jangka waktu panjang akan menyebabkan penumpukan data karena jumlah data guru dan data siswa yang cukup banyak maka data yang ditampung akan semakin besar dan memperlambat kinerja untuk menyajikan informasi secara cepat dan tepat. Penelitian ini bertujuan untuk mengembangkan sistem informasi akademik yang meliputi data guru, data siswa, data  kelas, jadwal kelas, dan nilai. Metodologi yang digunakan adalah Rational Unified Process dan pemodelan yang digunakan adalah Unified Modeling Language. Luaran dari penelitian ini adalah sistem informasi akademik sekolah SMK YABP 1 Garut berbasis web. Sistem informasi akademik berbasis web ini diharapkan dapat membantu memberikan informasi terkait data guru, data siswa, pendataan kelas, jadwal kelas dan nilai untuk pengajar, siswa, dan orang tua/wali siswa.
Las microempresas como entes generadores de empleo en el Ecuador
Authors: González-Ramón Ernesto , Ramírez-López Guido , Serdán Valencia Kendra , Morales-Ochoa Christian , Scaffy-Vivero Jahaira
Published: May 2022

El presente trabajo de investigación se realizó con el objetivo de analizar la incidencia que tienen las microempresas como entes generadores de empleo y su incidencia en el derecho del trabajo. La investigación es de carácter cualitativo con alcance descriptivo, la búsqueda de información se realizó a través de organismos oficiales. Los resultados reflejan que, en el año 2020 el 39% de los empleos en el Ecuador fueron generados por microempresas, sin embargo, esto no es del todo satisfactorio ya que otros estudios revelan irregularidades en las condiciones de trabajo existentes, pues según lo investigado se evidencia la baja calidad de empleos generados por las mismas, lo que impide a sus trabajadores acceder a un empleo digno y sustentable. Como conclusión se identificó que microempresas ecuatorianas podrían cumplir un papel aún más significativo para la economía del Ecuador, gracias a su incidencia en la generación de empleo y la dinamización económica, pero muchas de las veces el administrador realiza actos que incumplen con los derechos laborales del trabajador.
Análisis del e-wom y brand love del servicio de bar y restaurantes del sector hotelero
Authors: Sanchez-Moyon Nicole , Pineda-Cojitambo Diego , Sanchez-González Irene
Published: May 2022

La investigación analiza las variables del marketing -wom y brand love para analizar el servicio de bar y restaurantes del sector. Aplicando el método cuantitativo, se recopilaron 285 encuestas de consumidores de bares y restaurantes del sector hotelero de la ciudad de Machala, provincia de El Oro-Ecuador. Para el análisis de los resultados se emplearon los programas estadísticos SPSS y Smart PLS SEM 3.0. Los resultados apuntan a que las variables e-wom, sistema turístico, brand love, Marketing digital tienen un efecto positivo en la percepción y comportamiento de los consumidores, Se determina también que estos efectos influyen de manera significativa en la decisión de compra. Los resultados también brindan herramientas importantes al sector turístico y económico.
Los modelos administrativos en las PYMES del sector comercial de la ciudad de Machala
Authors: Sarango-Bermeo Magaly , Jaya-Pineda Iddar
Published: May 2022

Los modelos administrativos aportan con el desarrollo de las empresas, al momento de adaptarlas a sus propias necesidades ocasionan que sea constante su incidencia en la productividad y servicios que ofrece, porque permite obtener la descripción total sobre cómo se manejan las funciones dentro y fuera de la misma, buscando obtener la satisfacción de la administración, colaboradores y consumidores (clientes), con la finalidad de tomar la mejor decisión empresarial debido a la flexibilidad de cada modelo. El objetivo de la investigación es identificar los modelos administrativos aplicados en las pequeñas y medianas empresas de la ciudad de Machala, la utilidad de aplicarlos. Se aplicó el método cualitativo- descriptivo, para conocer la importancia de contar con un modelo administrativo, conociendo los beneficios que estos otorgan y por medio de una encuesta aplicada a las 50 empresas seleccionadas mediante el muestreo por conveniencia que se encuentran registradas en la Superintendencia de Compañías, Valores y Seguros. Los resultados obtenidos demuestran que los modelos administrativos aportan al mejoramiento interno y externo de las empresas porque están basados en estrategias que permiten cumplir las normas de calidad en la prestación de sus productos o servicios mediante la constante innovación empresarial para lograr las ganancias económicas anheladas, por ende, son considerados de gran importancia por las Pymes dedicadas a la actividad comercial.

Palabras claves: modelos administrativos, toma de decisión, Pymes, estrategias, innovación empresarial
van den Hoonaard, D. (2012). Qualitative Research in Action: A Canadian Primer. Oxford University Press. ISBN-10: 0199009473
Authors: Davies Adam
Published: May 2022

Qualitative Research in Action: A Canadian Primer (van den Hoonaard, 2012) provides a comprehensive introduction to qualitative research from a social constructivist, symbolic interactionism perspective. Van den Hoonaard references her previous writings and research experiences extensively as she provides context and examples for readers to draw upon while they learn new concepts and methodologies. Qualitative Research in Action is divided into ten chapters, and systematically goes through the process of beginning a new research project. She begins the preface of the text by locating her theoretical approach as being rooted in symbolic interactionism (p. x). The text moves into defining the differences between qualitative and quantitative work, as well as addressing the potential benefits of a mixed methods approach. Van den Hoonaard comes from a sociological perspective and presents Robert Prus’s (2005) ‘generic social processes’, which “reflect researchers’ interest in the emergent nature of human life” (p. 27). Moreover, she positions herself and Qualitative Research in Action as providing a uniquely Canadian perspective on qualitative research while citing Canadian qualitative studies.
Brookes, G., Pooley, J. A., & Earnest J. (2015). Terrorism, Trauma and Psychology: A multilevel victim perspective of the Bali bombings. New York, NY: Routledge.
Authors: Kovaliov Natalia
Published: May 2022

Dedicated to the victims of the 2002 Bali bombings, Terrorism, Trauma and Psychology: A multilevel victim perspective of the Bali bombings provides a comprehensive insight into the dimensionality of the effects experienced by those involved in terrorist attacks: victims, their family members, and volunteer and professional emergency responders. Intended as an essential reading for scholars in the fields of social and clinical psychology, victimology, and terrorist studies, this book is also a good background reading for those willing to learn how the Bali bombings of 2002 rank on the global scale of terrorist attacks.
IFRS applied by public sector entities: legislative regulation of property, plant and equipment transactions
Authors:   SHEE “Kyiv National Economic University named after Vadym Hetman”, NEBYLTSOVA Oksana , KORSHYKOVA Renata ,   SHEE “Kyiv National Economic University named after Vadym Hetman”
Published: May 2022

The article is dedicated to the issues of IFRS applied by public sector entities related to property, plant and equipment use as regard to their recognition, depreciation and derecognition. Specifying the general issue, our attention was focused on a certain range of aspects which are acquiring more and more importance in the activity of such kind of economic entities at both conceptual and action-oriented levels. A special consideration is given to some most challenging issues. Primarily, professional judgments implementation was required with the aim to select and apply accounting policy for assets obtained by state enterprises under the rights of economic management and permanent use which are specific legal regimes in post-Soviet countries. In its turn, it’s necessary to walk a line between IFRS and legislative acts of Ukraine’s requirements for addressing another issue concerning physically damaged, obsolescent, inoperable as a result of force-major without economic sense of recovery non-current assets derecognition. By means of applying a comparative analysis methodology, key differences regarding requirements towards depreciation policy of IAS 16 rules and National Accounting Standards 7 are discovered as well as its application by some public sector entities. It is discursive key components of property, plant and equipment depreciation policy with variable part which is being chosen voluntary by enterprise, namely, a depreciation method, useful life as well as salvage value. Having discovered the essence as well as advantages and disadvantages of key depreciation methods introduced by the above mentioned IAS, the dominant trends are revealed not just in Ukraine but in other countries including economically developed ones regarding, in particular, accelerated depreciation. Such kind of methods can be applied as an instrument of the state policy in the field of property, plant and equipment administration with the aim to accelerate their renovation and improve efficiency of performance . For further development above mentioned aspects, the consolidated approach in respect to property, plant and equipment decommission due to maintenance, modernization, modification, reconstruction or conservation through the prism of IFRS provisions and imperative rules of tax legislation of Ukraine is presented.
Способы участия в мировой истории после социализма
Authors: Рош Софи
Published: May 2022

Окончание холодной войны вынудило ученых в Центральной Азии обратиться к новым центрам власти, которые используют английский язык в качестве средства общения. На глобальном уровне участие и распределение ресурсов в академической сфере связаны с языковыми компетенциями. Эта тенденция оставляет мало места для постколониальных дебатов и критического осмысления недавнего прошлого. Одним из результатов стало чрезмерное внимание к национальности, в том числе в академических дискурсах. Однако помимо этой риторики мы находим взаимодействие с миром на гораздо более высоком уровне. У исследователей из Таджикистана мы находим работы, которые указывают на связи с соседними странами, общим персидским наследием, религиями и русским языковым и культурным наследием. Поступая таким образом, эти ученые выбирают участие в мире, которое философ Эдуар Глиссан описал для другого региона и которое он называет “Отношением” (relation). Участие здесь является ризомным способом связи с другими обществами, культурами и людьми при отказе от колониальных иерархий и линейной историчности.

The end of the cold war has forced scholars in Central Asia to turn to the new power centres that use the English language as way of communication. On a global level, participation and the distribution of resources in the academic field is linked to language competences. This trend leaves little space to engage in a post-colonial debate and critical reflection of the near past. One result was an overemphasis on nationality also in academic discourses. However, beyond this rhetoric we find an engagement with the world at a much larger level. Among scholars from Tajikistan, we find works that link to neighbouring countries, a shared Persian heritage, religious links, and Russian linguistic and cultural heritage. Doing so, those scholars have opted for a participation in the world that the philosopher Édouard Glissant has described for another region and that he terms “Relation”. This participation is a rhizome way of linking to other societies, cultures and people while rejecting colonial hierarchies and forms of linear historicity.
Efficient Production of Broad-Spectrum Antimicrobials by Paenibacillus polymyxa OSY–EC Using Acid Whey-Based Medium and Novel Antimicrobial Concentration Approach
Authors: Abdelhamid Ahmed G. , Campbell Emily P. , Hawkins Zach , Yousef Ahmed E.
Published: May 2022

Production of some antimicrobial peptides by bacterial producers is a resource-intensive process, thus, using inexpensive growth media and simplifying antimicrobial extraction and down-stream processing are highly desirable. Acid whey, a dairy industry waste, is explored as a medium for production of broad–spectrum antimicrobials from selected bacteriocinogenic bacteria. Neutralized and yeast extract-supplemented acid whey was suitable for production of antimicrobials by four tested strains, but Paenibacillus polymyxa OSY–EC was the most prolific antimicrobial producer. Concentrating synthesized antimicrobials during culture incubation using beads of polymeric adsorbent resin, followed by solvent extraction and freeze-drying, resulted in antimicrobials-rich powder (AMRP). Under these conditions, P. polymyxa OSY–EC produced paenibacillin, polymyxin E, and fusaricidin, which are active against Gram-positive and Gram-negative bacteria and fungi, respectively. When media containing 2x and 4x minimum inhibitory concentrations of AMRP were inoculated with Listeria innocua and Escherichia coli, microbial populations decreased by ≥4–log CFU ml−1 in tryptic soy broth and ≥3.5–log CFU ml−1 in milk. The antimicrobial mechanism of action of AMRP solutions was attributed to the disruption of cytoplasmic membrane of indicator strains, L. innocua and E. coli. These findings exemplify promising strategies for valorization of acid whey via microbial bioreactions to yield potent antimicrobials.
The Emerging Roles of Circ-ABCB10 in Cancer
Authors: Huang Zhenjun , Shan Renfeng , Wen Wu , Li Jianfeng , Zeng Xiaohong , Wan Renhua
Published: May 2022

Circular RNAs (circRNAs) are non-coding RNAs (ncRNAs) without 5′ caps and 3′ tails, which are formed from precursor mRNAs (pre-mRNAs) that are inversely back-spliced by exons. CircRNAs are characterized by a covalently closed circular structure and are abundantly expressed in eukaryotic cells. With the development of RNA-sequencing, it was discovered that circRNAs play important roles in the regulation of numerous human genes and are related to the occurrence, development, and prognosis of diseases. Studies in various cancers have revealed that circRNAs have both positive and negative effects on the occurrence and development of tumors. Circ-ABCB10, a circular RNA originating from exons of ABCB10 located on chromosome 1q42, has been proven to play an important role in different types of cancers. Here, we report the primary findings of recent research studies by many contributors about the roles of circ-ABCB10 in cancer and clearly formulate its influence and functions in different aspects of cancer biology, which gives us a broad picture of circ-ABCB10. Thus, this study aimed to generalize the roles of circ-ABCB10 in the diagnosis and treatment of different types of tumors and its related miRNA genes. In this way, we wish to provide a sufficient understanding and assess the future development direction of the research on circ-ABCB10.
Degradative and Non-Degradative Roles of Autophagy Proteins in Metabolism and Metabolic Diseases
Authors: Kuramoto Kenta , He Congcong
Published: May 2022

Autophagy is a stress-induced lysosomal degradation pathway regulated by evolutionarily conserved autophagy-related (ATG) genes. Recent research has revealed that autophagy plays an important role in the regulation of energy metabolism, development of metabolic tissues, and pathogenesis of metabolic disorders. Bulk and selective degradation by autophagy helps maintain protein homeostasis and physiological function of cells. Aside from classical degradative roles, ATG proteins also carry out non-classical secretory functions of metabolic tissues. In this review, we summarize recent progresses and unanswered questions on the mechanisms of autophagy and ATG proteins in metabolic regulation, with a focus on organelle and nutrient storage degradation, as well as vesicular and hormonal secretion. Such knowledge broadens our understanding on the cause, pathophysiology, and prevention of metabolic diseases including obesity and diabetes.
Pan-Cancer Analysis, Reveals COVID-19-Related BSG as a Novel Marker for Treatment and Identification of Multiple Human Cancers
Authors: Huang Tao , He Wei-Ying
Published: May 2022

Background: Coronavirus disease 2019 (COVID-19) has been a public threat and healthcare concern caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. During the period of the pandemic of COVID-19, cancer patients should be paid more attention as more severe events are found in cancer patients infected with SARS-CoV-2. Basigin (BSG) is an essential factor for the infection and progression of COVID-19 and tumorigenesis of multiple tumors, which may serve as a novel target for the effective treatment against COVID-19 and multiple human cancers.

Methods: A total of 19,020 samples from multiple centers were included in our research for the comprehensive investigation of the differences in BSG expression among human organs, cancer cells, cancer tissues, and normal tissues. Cox regression analysis and Kaplan–Meier curves were utilized to explore the prognosis factor of BSG in cancers. Correlation analyses were used to determine associations of BSG expression with tumor mutational burden, the immune microenvironment, etc. Gene set enrichment analysis was applied to explore the underlying mechanisms of BSG in cancers.

Results: Compared with normal tissues, BSG expression was high in 13 types of cancers (cholangiocarcinoma, etc.) and low in colon adenocarcinoma and rectum adenocarcinoma. BSG expression was related to the prognosis of eight cancers (e.g., invasive breast carcinoma) (p < 0.05). The gene also demonstrated a pronounced effect in identifying 12 cancers (cholangiocarcinoma, etc.) from their control samples (AUC >0.7). The BSG expression was associated with DNA methyltransferases, mismatch repair genes, immune infiltration levels, tumor mutational burden, microsatellite instability, neoantigen, and immune checkpoints, suggesting the potential of BSG as an exciting target for cancer treatment. BSG may play its role in several cancers by affecting several signaling pathways such as drug cytochrome metabolism P450 and JAK-STAT.

Conclusion:BSG may be a novel biomarker for treating and identifying multiple human cancers.
Neutrophil Recruitment in Pneumococcal Pneumonia
Authors: Palmer Catherine S. , Kimmey Jacqueline M.
Published: May 2022

Streptococcus pneumoniae (Spn)is the primary agent of community-acquired pneumonia. Neutrophils are innate immune cells that are essential for bacterial clearance during pneumococcal pneumonia but can also do harm to host tissue. Neutrophil migration in pneumococcal pneumonia is therefore a major determinant of host disease outcomes. DuringSpninfection, detection of the bacterium leads to an increase in proinflammatory signals and subsequent expression of integrins and ligands on both the neutrophil as well as endothelial and epithelial cells. These integrins and ligands mediate the tethering and migration of the neutrophil from the bloodstream to the site of infection. A gradient of host-derived and bacterial-derived chemoattractants contribute to targeted movement of neutrophils. During pneumococcal pneumonia, neutrophils are rapidly recruited to the pulmonary space, but studies show that some of the canonical neutrophil migratory machinery is dispensable. Investigation of neutrophil migration is necessary for us to understand the dynamics of pneumococcal infection. Here, we summarize what is known about the pathways that lead to migration of the neutrophil from the capillaries to the lung during pneumococcal infection.
Regional Strain Score as Prognostic Marker of Cardiovascular Events From the Multi-Ethnic Study of Atherosclerosis (MESA)
Authors: Pezel Theo , Bluemke David A. , Wu Colin O. , Lima João A. C. , Ambale Venkatesh Bharath
Published: May 2022

Background

Left ventricular (LV) circumferential strain (Ecc) is an accurate indicator of regional myocardial function, particularly using the regional Ecc or layer-specific strain.

Aim

This study aimed to investigate the prognostic value of a regional strain score (RSS) for predicting the incident of heart failure (HF) and coronary heart disease (CHD) in a population without a history of cardiovascular disease at baseline.

Materials and Methods

Data from participants in the Multi-Ethnic Study of Atherosclerosis (MESA) who underwent tagged magnetic resonance imaging for strain determination were analyzed. Using −17% and −10% as Ecc cut-offs, each segment was rated from 0 to 2 points according to the Ecc value of each layer. The endo-Ecc, mid-Ecc, and epi-Ecc values from the 16-segment model were used to calculate three RSS: Endo-, Mid-, and Epi-RSS, respectively, which were defined as a percentage of good LV regional function. The Intramyocardial-RSS was the sum of these three RSS. Cox proportional hazard models were used to evaluate the association between each RSS and incident HF and hard CHD.

Results

Among the 1,506 participants (63.3 ± 9.4 years, 54.6% men), 122 cases of hard CHD and 91 cases of HF were observed [median (IQR) follow-up 15.9 (12.9–16.6) years]. After adjustment, Mid-, Epi-, and Intramyocardial-RSS values <50% were independently associated with HF [adjusted HR 1.43; 95% CI (1.08–2.87), p = 0.004; HR 1.80; 95% CI (1.12–3.07), p < 0.001; and HR 2.01; 95% CI (1.19–3.20), p < 0.001]. After adjustment, Endo-, Mid-, Epi-, and Intramyocardial-RSS <50% were also independently associated with hard CHD [adjusted HR 1.31; 95% CI (1.03–1.51), p = 0.04; HR 1.79; 95% CI (1.26–2.57), p < 0.001; HR 2.03; 95% CI (1.45–3.40), p < 0.001; and HR 2.28; 95% CI (1.51–3.53), p < 0.001].

Conclusions

Layer-specific regional Ecc, assessed by RSS, provides a robust, independent predictive value for incident HF and hard CHD in asymptomatic participants without any history of previous clinical cardiovascular disease.

Clinical Trial Registration

Unique identifier: NCT00005487.
A Landscape of Metabonomics for Intermingled Phlegm and Blood Stasis and Its Concurrent Syndromes in Stable Angina Pectoris of Coronary Heart Disease
Authors: Zheng Li , Mingxue Zhang , Zeng Li , Yushi Zhou , Yuhan Ao , Yi Yang , Botong Liu
Published: May 2022

Objectives

In this study, we analyzed the metabonomics of intermingled phlegm and blood stasis (IPBS) and its three concurrent syndromes in patients with stable angina pectoris of coronary heart disease.

Methods

A total of 164 sera of separated outpatients from 12 national tradition Chinese medicine clinical research centers with IPBS or concurrent syndromes were collected for the study and assessed with LC-ESI-MS/MS (liquid chromatography—electrospray ionization tandem—mass spectrometry)-based metabolomics and multivariate statistical analysis.

Results

Non-differential metabolites between IPBS and its separate syndrome combined with the top 100 most abundant metabolites in four groups were screened to reflect the essence of IPBS. Amino acid and its metabolomics and glycerol phospholipids were screened for common metabolites, and these metabolites were mainly enriched in valine, leucine, and isoleucine metabolism and glycerophospholipid metabolism. Principal component analysis revealed that the difference between IPBS and its separate concurrent syndromes was not distinct. Compared with IPBS, anserine, cytidine 5′-diphosphocholine, and 7,8-dihydro-L-biopterin separately significant increase in phlegm stasis and toxin (PST), phlegm stasis and Qi stagnation (PQS), and phlegm stasis and Qi deficiency (PQD). While these different metabolites were associated with histidine metabolism, beta-alanine metabolism, glycerophospholipid metabolism, and folate biosynthesis. Three accurate identification models were obtained to identify the difference between IPBS and its concurrent syndromes.

Conclusion

Our study indicated that valine, leucine, and isoleucine metabolism and glycerophospholipid metabolism could represent the essence of IPBS; dysregulated metabolites were valuable in identifying PST from IPBS.
Deciphering Common Long QT Syndrome Using CRISPR/Cas9 in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Authors: Song Yongfei , Zheng Zequn , Lian Jiangfang
Published: May 2022

From carrying potentially pathogenic genes to severe clinical phenotypes, the basic research in the inherited cardiac ion channel disease such as long QT syndrome (LQTS) has been a significant challenge in explaining gene-phenotype heterogeneity. These have opened up new pathways following the parallel development and successful application of stem cell and genome editing technologies. Stem cell-derived cardiomyocytes and subsequent genome editing have allowed researchers to introduce desired genes into cells in a dish to replicate the disease features of LQTS or replace causative genes to normalize the cellular phenotype. Importantly, this has made it possible to elucidate potential genetic modifiers contributing to clinical heterogeneity and hierarchically manage newly identified variants of uncertain significance (VUS) and more therapeutic options to be tested in vitro. In this paper, we focus on and summarize the recent advanced application of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) combined with clustered regularly interspaced short palindromic repeats/CRISPR-associated system 9 (CRISPR/Cas9) in the interpretation for the gene-phenotype relationship of the common LQTS and presence challenges, increasing our understanding of the effects of mutations and the physiopathological mechanisms in the field of cardiac arrhythmias.
Rupture Risk Assessment for Anterior Communicating Artery Aneurysms Using Decision Tree Modeling
Authors: Liu Jinjin , Xing Haixia , Chen Yongchun , Lin Boli , Zhou Jiafeng , Wan Jieqing , Pan Yaohua , Yang Yunjun , Zhao Bing
Published: May 2022

Background

Although anterior communicating artery (ACoA) aneurysms have a higher risk of rupture than aneurysms in other locations, whether to treat unruptured ACoA aneurysms incidentally found is a dilemma because of treatment-related complications. Machine learning models have been widely used in the prediction of clinical medicine. In this study, we aimed to develop an easy-to-use decision tree model to assess the rupture risk of ACoA aneurysms.

Methods

This is a retrospective analysis of rupture risk for patients with ACoA aneurysms from two medical centers. Morphologic parameters of these aneurysms were measured and evaluated. Univariate analysis and multivariate logistic regression analysis were performed to investigate the risk factors of aneurysm rupture. A decision tree model was developed to assess the rupture risk of ACoA aneurysms based on significant risk factors.

Results

In this study, 285 patients were included, among which 67 had unruptured aneurysms and 218 had ruptured aneurysms. Aneurysm irregularity and vessel angle were independent predictors of rupture of ACoA aneurysms. There were five features, including size ratio, aneurysm irregularity, flow angle, vessel angle, and aneurysm size, selected for decision tree modeling. The model provided a visual representation of a decision tree and achieved a good prediction performance with an area under the receiver operating characteristic curve of 0.864 in the training dataset and 0.787 in the test dataset.

Conclusion

The decision tree model is a simple tool to assess the rupture risk of ACoA aneurysms and may be considered for treatment decision-making of unruptured intracranial aneurysms.
Strategies to Improve the Targeting of Retinal Cells by Non-Viral Gene Therapy Vectors
Authors: Bitoque Diogo B. , Fernandes Cláudia F. , Oliveira Alexandra M. L. , Silva Gabriela A.
Published: May 2022

Retinal diseases lead to severe vision loss and are currently a major cause of vision impairment in industrialized countries. The significant number of genetic defects of the retina underlying these disorders, coupled to the absence of effective treatments, require new therapeutic solutions. Recent gene therapy developments in the field of ophthalmic research reveal the great potential of this approach. In recent years, non-viral vectors have been extensively studied due to their properties such as large gene packaging capacity and low immunogenicity. Hitherto, their development and optimisation for retinal gene therapy have been hindered by their inability to directly target retinal cells. The goal of this review is to summarize the most promising strategies to direct non-viral vectors for retinal cells to avoid off-target effects and promote their specific uptake, gene expression and overall efficiency.
Modulating the Immune Response in Periodontitis
Authors: Bezerra Beatriz , Monajemzadeh Sepehr , Silva Davi , Pirih Flavia Q.
Published: May 2022

Periodontitis is a chronic inflammatory condition initiated by the accumulation of bacterial biofilm. It is highly prevalent and when left untreated can lead to tooth loss. The presence of bacterial biofilm is essential for the initiation of the inflammatory response but is not the sole initiator. Currently it is unknown which mechanisms drive the dysbiosis of the bacterial biofilm leading to the dysregulation of the inflammatory response. Other players in this equation include environmental, systemic, and genetic factors which can play a role in exacerbating the inflammatory response. Treatment of periodontal disease consists of removal of the bacterial biofilm with the goal of resolving the inflammatory response; however, this does not occur in every case. Understanding the way the inflammatory response does not return to a state of homeostasis has led investigators to consider both systemic and local pharmacological interventions. Nonetheless, a better understanding of the impact that genetics and environmental factors may have on the inflammatory response could be key to helping identify how inflammation can be modulated therefore stopping the destruction of the periodontium. In this article, we will explore the current evidence associating the microbial dysbiosis and the dysregulation of the immune response, potential mechanisms or pathways that may be targeted for the modulation of the inflammatory response, and discuss the advantages and drawbacks associated with local and systemic inflammatory modulation in the management of periodontal disease. This information will be valuable for those interested in understanding potential adjunct methods for managing periodontal diseases, but not limited to, dental professionals, clinical researchers and the public at large.
Evaluating the Association of Regional and City-Level Environmental Greenness and Land Over Patterns With PM2.5 Pollution: Evidence From the Shanxi Province, China
Authors: Guo Guangxing , Liu Liwen , Duan Yonghong
Published: May 2022

Ambient PM2.5 (fine particulate matter with aerodynamic diameters ≤2.5 μm) is a major threat to human health. Environmental fates and human exposure to PM2.5 can be affected by various factors, and environmental greenness have been documented to be significantly associated with the exposure disparities; however, the relationship between the greenness and ambient PM2.5 on the region and city levels, and variations across different land cover types remain unclear. In this study, PM2.5 changes from 2001 to 2020 varying over different land cover types and cities were analyzed, and discussed for the relationships with environmental greenness, by taking Shanxi province as an example. The results showed in the past 2 decades, the mean annual NDVI (normalized difference vegetation index) of the study area showed a significant increasing trend (p < 0.01), and the PM2.5 concentration decreased as environmental greenness get better. The same trends were observed across different land cover types and cities. The negative correlation was stronger in the construction land with more frequent human activities, especially in the built-up areas with low vegetation coverage; but limited in the high green space coverage areas. These results provide quantitative decision-making references for the rational development, utilization and management of land resources, but also achieving regional coordinated controls of PM2.5 pollution by optimizing land use.
Ontogeny, Phylotypic Periods, Paedomorphosis, and Ontogenetic Systematics
Authors: Martynov Alexander , Lundin Kennet , Korshunova Tatiana
Published: May 2022

The key terms linking ontogeny and evolution are briefly reviewed. It is shown that their application and usage in the modern biology are often inconsistent and incorrectly understood even within the “evo-devo” field. For instance, the core modern reformulation that ontogeny not merely recapitulates, but produces phylogeny implies that ontogeny and phylogeny are closely interconnected. However, the vast modern phylogenetic and taxonomic fields largely omit ontogeny as a central concept. Instead, the common “clade-” and “tree-thinking” prevail, despite on the all achievements of the evo-devo. This is because the main conceptual basis of the modern biology is fundamentally ontogeny-free. In another words, in the Haeckel’s pair of “ontogeny and phylogeny,” ontogeny is still just a subsidiary for the evolutionary process (and hence, phylogeny), instead as in reality, its main driving force. The phylotypic periods is another important term of the evo-devo and represent a modern reformulation of Haeckel’s recapitulations and biogenetic law. However, surprisingly, this one of the most important biological evidence, based on the natural ontogenetic grounds, in the phylogenetic field that can be alleged as a “non-evolutionary concept.” All these observations clearly imply that a major revision of the main terms which are associated with the “ontogeny and phylogeny/evolution” field is urgently necessarily. Thus, “ontogenetic” is not just an endless addition to the term “systematics,” but instead a crucial term, without it neither systematics, nor biology have sense. To consistently employ the modern ontogenetic and epigenetic achievements, the concept of ontogenetic systematics is hereby refined. Ontogenetic systematics is not merely a “research program” but a key biological discipline which consistently links the enormous biological diversity with underlying fundamental process of ontogeny at both molecular and morphological levels. The paedomorphosis is another widespread ontogenetic-and-evolutionary process that is significantly underestimated or misinterpreted by the current phylogenetics and taxonomy. The term paedomorphosis is refined, as initially proposed to link ontogeny with evolution, whereas “neoteny” and “progenesis” are originally specific, narrow terms without evolutionary context, and should not be used as synonyms of paedomorphosis. Examples of application of the principles of ontogenetic systematics represented by such disparate animal groups as nudibranch molluscs and ophiuroid echinoderms clearly demonstrate that perseverance of the phylotypic periods is based not only on the classic examples in vertebrates, but it is a universal phenomenon in all organisms, including disparate animal phyla.
Combined Carbon and Albedo Climate Forcing From Pine and Switchgrass Grown for Bioenergy
Authors: Ahlswede Benjamin J. , O'Halloran Thomas L. , Thomas R. Quinn
Published: May 2022

Expanding and restoring forests decreases atmospheric carbon dioxide, a natural solution for helping mitigate climate change. However, forests also have relatively low albedo compared to grass and croplands, which increases the amount of solar energy they absorb into the climate system. An alternative natural climate solution is to replace fossil fuels with bioenergy. Bioenergy crops such as switchgrass have higher albedo than forest ecosystems but absorb less total carbon over their lifetime. To evaluate trade-offs in the mitigation potential by pine and switchgrass ecosystems, we used eddy covariance net ecosystem exchange and albedo observations collected from planted pine forests and switchgrass fields in eastern North America and Canada to compare the net radiative forcing of these two ecosystems over the length of typical pine rotation (30 years). We found that pine had a net positive radiative forcing (warming) of 5.4 ± 2.8 Wm−2 when albedo and carbon were combined together (30 year mean). However the assumptions regarding the fate of harvested carbon had an important effect on the net radiative forcing. When we assumed all switchgrass carbon was emitted to the atmosphere while the harvested pine carbon was prevented from entering the atmosphere, the 30-year mean net radiative forcing reversed direction (−3.6 ± 2.8 Wm−2). Overall, while the pine ecosystem absorbed more carbon than the switchgrass, the difference in albedo was large enough to result in similar climate mitigation potential at the 30-year horizon between the two systems, whereby the direction and magnitude of radiative forcing depends on the fate of harvested carbon.