bluuebunny/crossref_metadata_2025 huggingface dataset:
An Update on Prevalence, Assessment, and Risk Factors for Sleep Disturbances in Patients with Advanced Cancer—Implications for Health Care Providers and Clinical Research
Authors: Jakobsen Gunnhild , Gjeilo Kari Hanne , Hjermstad Marianne Jensen , Klepstad Pål
Published: August 2022

Patients with advanced cancer experience multiple symptoms, with fluctuating intensity and severity during the disease. They use several medications, including opioids, which may affect sleep. Sleep disturbance is common in cancer patients, decreases the tolerability of other symptoms, and impairs quality of life. Despite its high prevalence and negative impact, poor sleep quality often remains unrecognized and undertreated. Given that sleep is an essential aspect of health-related quality of life, it is important to extend both the knowledge base and awareness among health care providers in this field to improve patient care. In this narrative review, we provide recommendations on sleep assessment in patients with advanced cancer and highlight cancer-related factors that contribute to insomnia. We also present direct implications for health care providers working in palliative care and for future research.
The Effect of DNA from Escherichia Coli at High and Low CO2 Concentrations on the Shape and Form of Crystal-line Silica-Carbonates of Barium (II)
Authors: Pérez-Aguilar Cesia D. , Islas Selene R. , Moreno Abel , Cuéllar-Cruz Mayra
Published: August 2022

The synthesis of nucleic acids in the Precambrian era marked the start of life, with DNA being the molecule in which the genetic information has been conserved ever since. After studying the DNA of different organisms for several decades, we now know that cell size and cellular differentiation are influenced by DNA concentration and environmental conditions. However, we still need to find out the minimum required concentration of DNA in the pioneer cell to control the resulting morphology. In order to do this, the present research aims to evaluate the influence of the DNA concentration on the morphology adopted by biomorphs (barium silica-carbonates) under two synthesis conditions: one emulating the Precambrian era and one emulating the present era. The morphology of the synthetized biomorphs was assessed through scanning electron microscopy (SEM). The chemical composition and the crystalline structure were determined through Raman and IR spectroscopy. Our results showed that DNA, even at relatively low levels, affects the morphology of the biomorph structure. They also indicated that, even at the low DNA concentration prevailing during the synthesis of the first DNA biomolecules existing in the primitive era, these biomolecules influenced the morphology of the inorganic structure that lodged it. On the other hand, this also allows us to infer that, once the DNA was synthetized in the Precambrian era, it was definitely responsible for generating, conserving, and directing the morphology of all organisms up to the present day.
Survival Comparisons between Breast Conservation Surgery and Mastectomy Followed by Postoperative Radiotherapy in Stage I–III Breast Cancer Patients: Analysis of the Surveillance, Epidemiology, and End Results (Seer) Program Database
Authors: Xiang Wenbin , Wu Chaoyan , Wu Huachao , Fang Sha , Liu Nuomin , Yu Haijun
Published: August 2022

Background: This study aims to evaluate the overall and breast cancer-specific survival (BCSS) after breast-conserving surgery (BCS) plus radiotherapy (RT) compared with mastectomy plus RT in resectable breast cancer. Moreover, the aim is to also identify the subgroups who benefit from BCS plus RT and establish a predictive nomogram for stage II patients. Methods: Stage I–III breast cancer patients were identified from the Surveillance, Epidemiology, and End Results (SEER) database between 1990 and 2016. Patients with available clinical information were split into two groups: BCS plus RT and mastectomy plus RT. Kaplan–Meier survival analysis, univariate and multivariate regression analysis, and propensity score matching were used in the study. Hazard ratio (HR) was calculated based on stratified Cox univariate regression analyses. A prognostic nomogram by multivariable Cox regression model was developed for stage II patients, and consistency index (C-index) and calibration curve were used to evaluate the accuracy of the nomogram in the training and validation set. Results: A total of 24,590 eligible patients were enrolled. The difference in overall survival (OS) and BCSS remained significant in stage II patients both before and after PSM (after PSM: OS: HR = 0.8536, p = 0.0115; BCSS: HR = 0.7803, p = 0.0013). In stage II patients, the survival advantage effect of BCS plus RT on OS and BCSS was observed in the following subgroups: any age, smaller tumor size (<1 cm), stage IIA (T2N0, T0–1N1), ER (+), and any PR status. Secondly, the C-indexes for BCSS prediction was 0.714 (95% CI 0.694–0.734). The calibration curves showed perfect agreement in both the training and validation sets. Conclusions: BCS plus RT significantly improved the survival rates for patients of stage IIA (T2N0, T0–1N1), ER (+). For stage II patients, the nomogram was a good predictor of 5-, 10-, and 15-year BCSS. Our study may help guide treatment decisions and prolong the survival of stage II breast cancer patients.
Layout Comparison and Parameter Optimization of Supercritical Carbon Dioxide Coal-Fired Power Generation Systems under Environmental and Economic Objectives
Authors: Chen Dongxu , Han Zhonghe , Bai Yaping , Guo Dongyang , Zhao Linfei , Li Peng
Published: August 2022

In the current studies, the supercritical carbon dioxide coal-fired power generation systems show efficiency and cost advantages over the traditional steam-based power systems. However, few studies have considered simultaneously environmental and economic objectives in the multi-objective analysis process. This study conducts a layout comparison and parameter optimization of the systems under the above two objectives. Initially, the thermodynamic, environmental, and economic models of the systems are established. Subsequently, the optimal layout is determined by the two-stage layout comparison. Further, multi-objective optimization is performed for the selected layout, and the optimal design parameters are determined by the decision process. Finally, the sensitivities of three selected parameters to the optimization results are analyzed. The results show that the basic layout coupled with overlap and intercooling schemes is optimal. Its ultimate environmental impact (UEI) and levelized cost of electricity (LCOE) are 219.8 kp-eq and 56.9 USD/MWh, respectively. The two objectives UEI and LCOE are conflicting. Based on a trade-off between them, the maximum temperature/pressure of the system is determined to be 635.3 °C/30.1 MPa. The coal price per unit of heat shows the highest sensitivity, and the pinch temperature difference of the recuperator shows opposite sensitivities at the UEI below 218 kp-eq and above 223 kp-eq.
A Dead Reckoning Calibration Scheme Based on Optimization with an Adaptive Quantum-Inspired Evolutionary Algorithm for Vehicle Self-Localization
Authors: Yu Biao , Zhu Hui , Xue Deyi , Xu Liwei , Zhang Shijin , Li Bichun
Published: August 2022

Parameter calibration is critical for self-localization based on dead reckoning in the control of intelligent vehicles such as autonomous driving. Most traditional calibration methods for robotics control based on dead reckoning rely on data collection with specially designed paths. For the calibration of parameters in the control of intelligent vehicles, the design of such paths is considered impossible due to the complexity of road conditions. To solve this problem, an optimization-based dead reckoning calibration scheme is introduced in this research using the differential global positioning system to obtain the actual positions of the intelligent vehicle. In this scheme, the difference between the positions obtained through dead reckoning and the positions obtained through the differential global positioning system is selected as the optimization objective function to be minimized. An adaptive quantum-inspired evolutionary algorithm is developed to improve the quality and efficiency of optimization. Experiments with an intelligent vehicle were also conducted to demonstrate the effectiveness of the developed calibration scheme. In addition, the newly introduced adaptive quantum-inspired evolutionary algorithm is compared with the classic genetic algorithm and the classic quantum-inspired evolutionary algorithm using eight benchmark test functions considering computation quality and efficiency.
Numerical Simulation for Redundant Electro-Hydrostatic Servo-Actuators under Certain Special Conditions
Authors: Dinca Liviu , Bogateanu Radu , Corcau Jenica-Ileana , Dumitrache Alexandru , Suatean Bogdan
Published: August 2022

This work presents some numerical simulations, performed using AMESim software, for a system composed by two redundant servo-actuators. Certain operating conditions are taken into account for these two electro-hydrostatic servo-actuators, which are coupled to the command surface (aileron) of a transport aircraft. We first considered the situation of slight asymmetries in the construction of the servo-actuators, then a situation in which one servo-actuator fails at maximum flight speed, and then at a medium flight speed, and finally we considered the effect of gusts upon the system. Small differences in the construction of the servo-actuators were taken into consideration by modifying each of the pump displacements, one by +2% and one by −2%, from the nominal value. It is possible that many other asymmetries exist in the construction of servo-actuators, such as different liquid leakages in the cylinders or pumps, small differences in the controller coefficients, and so on. These differences should be taken into account in future works. Our results provide evidence that, under some operation situations of redundant servo-actuators, significant overstresses can appear in one servo-actuator, leading to a decrease in the time for which the system operates correctly.
Green Credit of China’s Coal Power Enterprises during Green Transformation: A Tripartite Evolutionary Game Analysis
Authors: Cui Feng , Han Chuanfeng , Liu Pihui , Teng Minmin
Published: August 2022

China’s green credit has mostly been invested in new energy areas with positive environmental externalities in recent years, while coal power enterprises have been neglected. This paper constructs a tripartite evolutionary game model among government, coal power enterprises, and banks to clarify the key factors and mechanisms for coal power enterprises undergoing green transformation. The research results show that: Firstly, to realize the spontaneous green transformation of coal power enterprises, spontaneous profitability must be achieved before the removal of policy incentives, which is reflected in the continuous increase in electricity price, carbon emission trading price, and decrease in green transformation cost. Secondly, the green credit adjustment factor cannot determine whether a company chooses to undertake green transition, but it provides a valuable window of green transition for companies. When the relative benefits of green transformation projects are greater than the relative costs, coal power enterprises will undergo green transformation spontaneously. Thirdly, lower green credit interest rates are not better. An optimal green credit interest rate exists, that allows coal power enterprises to obtain the longest transition window in which to achieve spontaneous profitability for green transition projects.
Optimal Operation Strategy of PV-Charging-Hydrogenation Composite Energy Station Considering Demand Response
Authors: Zhu Liwen , He Jun , He Lixun , Huang Wentao , Wang Yanyang , Liu Zong
Published: August 2022

Traditional charging stations have a single function, which usually does not consider the construction of energy storage facilities, and it is difficult to promote the consumption of new energy. With the gradual increase in the number of new energy vehicles (NEVs), to give full play to the complementary advantages of source-load resources and provide safe, efficient, and economical energy supply services, this paper proposes the optimal operation strategy of a PV-charging-hydrogenation composite energy station (CES) that considers demand response (DR). Firstly, the operation mode of the CES is analyzed, and the CES model, including a photovoltaic power generation system, fuel cell, hydrogen production, hydrogen storage, hydrogenation, and charging, is established. The purpose is to provide energy supply services for electric vehicles (EVs) and hydrogen fuel cell vehicles (HFCVs) at the same time. Secondly, according to the travel law of EVs and HFCVs, the distribution of charging demand and hydrogenation demand at different periods of the day is simulated by the Monte Carlo method. On this basis, the following two demand response models are established: charging load demand response based on the price elasticity matrix and interruptible load demand response based on incentives. Finally, a multi-objective optimal operation model considering DR is proposed to minimize the comprehensive operating cost and load fluctuation of CES, and the maximum–minimum method and analytic hierarchy process (AHP) are used to transform this into a linearly weighted single-objective function, which is solved via an improved moth–flame optimization algorithm (IMFO). Through the simulation examples, operation results in four different scenarios are obtained. Compared with a situation not considering DR, the operation strategy proposed in this paper can reduce the comprehensive operation cost of CES by CNY 1051.5 and reduce the load fluctuation by 17.8%, which verifies the effectiveness of the proposed model. In addition, the impact of solar radiation and energy recharge demand changes on operations was also studied, and the resulting data show that CES operations were more sensitive to energy recharge demand changes.
Estimation of Ignition Pressure in Ammunition
Authors: Trębiński Radosław , Woźniak Ryszard , Szupieńko Damian , Fikus Bartosz
Published: August 2022

This paper presents the experimental estimation of the ignition pressure value produced by a primer in small arms ammunition. Investigations were performed using a chamber of the capacity equal to the capacity of the 12.7 mm ammunition case. The inner volume of the chamber was adjusted to the free volume in the cartridge by inserting a sleeve or glass balls of a diameter of 2 or 4 mm. The pressure values were measured inside the chamber after the action of the primer. The maximum pressure value was used as an estimation of the ignition pressure value. Depending on the nature of the filling, considerably different values were obtained; they were compared with the values of pressure measured inside the case at the live firing. A considerable difference between the ignition pressure values measured inside the case and those measured in the model setup was detected. Based on these results, a discussion concerning the choice of the starting pressure in interior ballistics lumped parameters models was carried out.
Simulation Analysis of Issues with Grid Disturbance for a Photovoltaic Powered Virtual Synchronous Machine
Authors: Takamatsu Takahiro , Oozeki Takashi , Orihara Dai , Kikusato Hiroshi , Hashimoto Jun , Otani Kenji , Matsuura Takahiro , Miyazaki Satoshi , Hamada Hiromu , Miyazaki Teru
Published: August 2022

The increase in inverter-based resources associated with the increased installation of PV sources is a concern because it reduces the inertia of the power system during peak PV generation periods. As a countermeasure to reduce grid inertia, the addition of pseudo-inertia using virtual synchronous machines can be selected, and PV generation can cooperatively contribute to the stable operation of the power system by using the suppressed output as reserve power. However, few studies have analyzed VSMs that do not use batteries and use PV as a resource (PV-VSM) in simulations, including grid interconnection and solar radiation fluctuations, and it is necessary to clarify the issues and discuss countermeasures. In this study, electromagnetic transient response analysis was applied to a VSM connected to a two-generator system, simulations were performed, and the following findings were reported and countermeasure methods for the problem were proposed. When the PV capacity is insufficient for the output required by the VSM inverter, the PV-VSM control system may become unstable. This is caused by a drop in the capacitor voltage of the DC/DC converter due to insufficient PV output. The limiter control system is designed to address this problem by combining the headroom estimation system with the current limiting algorithm. The proposed limiter control system is validated on solar radiation ramp fluctuations as a test case and found that the system was effective in supressing PV-VSM instability. In our simulation case, the PV-VSM with our limiter control can continue to operate stably even if the PV available power is 0.03 [p.u.] short of the inverter’s reference power by the solar power ramp fluctuation, as long as the inverter installation rate is less than 50%.
Analysis of the Impact of Flooring Material and Construction Solutions on Heat Exchange with the Ground in a Historic Wooden Building
Authors: Sokołowski Paweł , Nawalany Grzegorz , Michalik Małgorzata
Published: August 2022

The article deals with the issue of the influence of selected material and construction solutions for a floor in a historic wooden building on heat exchange with the ground. The scope of the work included continuous measurements of selected parameters of internal and external microclimate, which were later used for numerical analysis of selected calculation variants. The research was carried out in a historic wooden church located in southern Poland. The research period covered 2019, while all measurements were performed every 1 h. For the variant analysis, a building with a wooden and stone floor was adopted. The influence of the heating system on the heat exchange with the ground for wooden and stone floors was also analysed. As a result of a detailed analysis, it was found that the material and construction solutions, as well as the heating system, have a significant impact on the formation of heat exchange with the ground. The building with a wooden floor was characterised by significantly higher values of energy losses to the ground in relation to heat gains. During the year, the total energy losses to land amounted to 1005 kWh, while the gain was 47 kWh. The energy flow from inside the building to the ground in August was 2.4 times higher in variant 2 than in variant 1. In February, heat losses to the ground were 1.6 times higher in variant 2 compared to variant 1.
Rumen Metaproteomics Highlight the Unique Contributions of Microbe-Derived Extracellular and Intracellular Proteins for In Vitro Ruminal Fermentation
Authors: Shi Tao , Guo Xi , Liu Yuqin , Zhang Tingting , Wang Xiangnan , Li Zongjun , Jiang Yu
Published: August 2022

Rumen microorganisms can be used in in vitro anaerobic fermentation to encourage the sustainable exploitation of agricultural wastes. However, the understanding of active microbiota under in vitro ruminal fermentation conditions is still insufficient. To investigate how rumen microbes actively participate in the fermentation process in vitro, we resolved the metaproteome generated from ruminal fermentation broth after seven days of in vitro incubation. Herein, the sample-specific database for metaproteomic analysis was constructed according to the metagenomic data of in vitro ruminal fermentation. Based on the sample-specific database, we found in the metaproteome that Bacteroidetes and Firmicutes_A were the most active in protein expression, and over 50% of these proteins were assigned to gene categories involved in energy conversion and basic structures. On the other hand, a variety of bacteria-derived extracellular proteins, which contained carbohydrate-active enzyme domains, were found in the extracellular proteome of fermentation broth. Additionally, the bacterial intracellular/surface moonlighting proteins (ISMPs) and proteins of outer membrane vesicles were detected in the extracellular proteome, and these ISMPs were involved in maintaining microbial population size through potential adherence to substrates. The metaproteomic characterizations of microbial intracellular/extracellular proteins provide new insights into the ability of the rumen microbiome to maintain in vitro ruminal fermentation.
A Novel Logo Identification Technique for Logo-Based Phishing Detection in Cyber-Physical Systems
Authors: Panda Padmalochan , Mishra Alekha Kumar , Puthal Deepak
Published: August 2022

The first and foremost task of a phishing-detection mechanism is to confirm the appearance of a suspicious page that is similar to a genuine site. Once this is found, a suitable URL analysis mechanism may lead to conclusions about the genuineness of the suspicious page. To confirm appearance similarity, most of the approaches inspect the image elements of the genuine site, such as the logo, theme, font color and style. In this paper, we propose a novel logo-based phishing-detection mechanism that characterizes the existence and unique distribution of hue values in a logo image as the foundation to unambiguously represent a brand logo. Using the proposed novel feature, the detection mechanism optimally classifies a suspicious logo to the best matching brand logo. The experiment is performed over our customized dataset based on the popular phishing brands in the South-Asia region. A set of five machine-learning algorithms is used to train and test the prepared dataset. We inferred from the experimental results that the ensemble random forest algorithm achieved the high accuracy of 87% with our prepared dataset.
Nowcasting GDP: An Application to Portugal
Authors: Assunção João B. , Fernandes Pedro Afonso
Published: August 2022

Forecasting the state of an economy is important for policy makers and business leaders. When this is conducted in real-time, it is called nowcasting. In this paper, we present a method that shows how forecasting errors decline as additional contemporaneous information unfolds and becomes available. When the economic environment changes fast, as has happened often in the last decades across most developed economies, it is important to use forecasting methods that are both flexible and robust. This can be achieved with bridge equations and non-parametric estimates of the trend growth using only publicly available information. The method presented in this paper achieves, by the end of a quarter, an accuracy that is equivalent to the methods used by official entities.
Comparative Transcriptome Analysis Reveals Genes Associated with the Gossypol Synthesis and Gland Morphogenesis in Gossypium hirsutum
Authors: Zhang Cuiping , Liu Xiuyan , Song Yin , Sun Zhengran , Zhang Jinli , Wu Hao , Yang Yuzhen , Wang Zhenkai , He Daohua
Published: August 2022

Gossypium hirsutum is an important source of natural textile fibers. Gossypol, which is a sesquiterpenoid compound mainly existing in the cotton pigment glands, can facilitate resistance to the stress from diseases and pests. The level of gossypol in the cotton is positively correlated to the quantity of pigment glands. However, the underlying regulatory mechanisms of gossypol synthesis and gland morphogenesis are still poorly understood, especially from a transcriptional perspective. The transcripts of young leaves and ovules at 30 DPA of the glanded plants and glandless plants were studied by RNA-Seq and 865 million clean reads were obtained. A total of 34,426 differentially expressed genes (DEGs) were identified through comparative transcriptome analysis. Genes related to gossypol synthesis or gland morphogenesis displayed significant differential expression between the two cultivars. Functional annotation revealed that the candidate genes related to catalytic activity, the biosynthesis of secondary metabolites, and biomolecular decomposition processes. Our work herein unveiled several potential candidate genes related to gossypol synthesis or gland morphogenesis and may provide useful clues for a breeding program of cotton cultivars with low cottonseed gossypol contents.
Bark Inclusions in Canes of Southern Highbush Blueberry and Their Impact on Cane Union Strength and Association with Botryosphaeria Stem Blight
Authors: Holland Renee M. , Peterson Chris J. , Harmon Philip F. , Brannen Phillip M. , Scherm Harald
Published: August 2022

Bark inclusions are an understudied structural defect in trees and shrubs. They consist of areas of bark on adjacent parts of stems or scaffolds, typically on the inner faces of a narrow fork, which become overgrown and internalized to occupy part of the wood between the stems. Here, bark inclusions are described for the first time to occur in cane unions at the crown of southern highbush blueberry (Vaccinium corymbosum interspecific hybrids) cultivars ‘Farthing’ and ‘Meadowlark’, both of which are characterized by a narrow, vase-shaped architecture at the base of the plant, leading to crowding of the canes. When affected canes were dissected at their bases, bark inclusions were visible internally as a line of compressed bark within the wood of adjoining canes, or as bark invaginations and fissures across part of or the entire cross-section of the cane. Externally, blueberry crowns with included bark were characterized by either an inward ridgeline of bark between canes of similar diameters emerging from the crown at a narrow angle from each other, or by the presence of girdling roots. Bark inclusions were observed in plants of all ages, from the nursery to mature production fields. The internal length of the bark inclusion correlated strongly with the external length of the inward stem bark ridgeline symptom as measured by destructive sampling in the field (r = 0.916, p < 0.0001, n = 20). When plants with and without bark inclusions were subjected to a winch test in the field, the probability of breakage for canes without included bark was significantly lower (p < 0.0002) than for those with included bark, and at the maximum applied force of 972.4 N, 95.2% of the canes with bark inclusions failed (i.e., broke at the crown), compared with only 52.6% for canes without included bark. In a survey across three fields, the number of bark inclusions per plant was significantly associated with an index of cane crowding (r = 0.286. p = 0.0267, n = 60), suggesting that plants with tight, crowded bases had more bark inclusions. In addition, there was a significant association (p < 0.0001) between the presence or length of bark inclusions and the intensity of Botryosphaeria stem blight in these fields. This study showed that bark inclusions occur commonly in certain southern highbush blueberry cultivars in the production conditions of Georgia and Florida, with negative implications for cane integrity and plant health.
A Simulation Study on the Processes of Intra-Group Informal Interaction Affecting Workers’ Safety Behaviors
Authors: Chen Huihua , Chen Cong , Li Hujun , Zhang Jianshe , Yang Zengke
Published: August 2022

The construction industry across the world is characterized by a high safety risk, and the occurrence of these safety accidents has led to substantial economic and social losses. The workers’ unsafe behaviors are considered to be a main cause. Thus, recently, scholars in the construction industry have shifted their attention to the investigation of the influencing factors (or antecedents) and their impact on workers’ safety behaviors (WSBs), hoping to provide insight into useful management policies. The existing literature has identified many society-level, cooperation-level, project-level, and individual-level concepts influencing WSB, but ignores the influence of intra-group informal interaction (IGII) on WSB. This study constructed a conceptual model for IGII, group knowledge sharing (GKS), and group identification (GI) to determine their influence on construction workers’ safety behaviors, and then conducted simulation analysis using the software of NetLogo. The results show that IGII, GKS, and GI can positively influence workers’ safety behaviors, and IGII can also positively influence WSB through GKS and GI. This study enriches the theoretical knowledge on the causation of construction workers’ safety behaviors, provides references for project managers to carry out proper safety management, and offers a theoretic foundation for the formulation of industry regulations.
Functioning of the EROS-R Scale in a Clinical Sample of Psychiatric Patients: New Psychometric Evidence from the Classical Test Theory and the Item Response Theory
Authors: Vilca Lindsey W. , Chambi-Mamani Evelyn L. , Quispe-Kana Emely D. , Hernández-López Mónica , Caycho-Rodríguez Tomás
Published: August 2022

Reliable and valid assessment instruments that can be applied briefly and easily in clinical and outpatient settings that provide information about the sources of reinforcement that the patient finds in his life are especially relevant in therapy. The study aimed to evaluate the psychometric properties of the Environmental Reward Observation Scale (EROS-R) in a sample of psychiatric patients. A sample of 228 psychiatric patients of both sexes (56.1% men and 43.9% women) aged between 18 and 70 years was selected. Along with the EROS-R, other instruments were administered to assess depression and anxiety. The results show that the scale fits a unidimensional model, presenting adequate fit indices (RMSEA = 0.077 (IC 90% 0.055–0.100); SRMR = 0.048; CFI = 0.98; TLI = 0.98). It was also shown that the degree of reward provided by the environment (EROS-R) correlates negatively with the level of depression (ρ = −0.54; p < 0.01) and anxiety (ρ = −0.34; p < 0.01). From the IRT perspective, all the items present adequate discrimination indices, where item 4 is the most precise indicator to measure the degree of environmental reward. All this leads us to conclude that the EROS-R is an instrument with robust psychometric guarantees from TCT and IRT’s perspectives, making it suitable for use in clinical contexts.
Classification of Deprivation Indices That Applied to Detect Health Inequality: A Scoping Review
Authors: Zelenina Anastasia , Shalnova Svetlana , Maksimov Sergey , Drapkina Oksana
Published: August 2022

Introduction: Many studies around the world are undertaken to establish the association between deprivation and public health indicators. Both separate indicators (e.g., income, education, occupation, public security and social support) and complex models (indices) include several indicators. Deprivation indices are actively used in public health since the mid 1980s. There is currently no clear classification of indices. Methods: In the current review, data related to deprivation indices are combined and analyzed in order to create a taxonomy of indices based on the results obtained. The search was carried out using two bibliographic databases. After conducting a full-text review of the articles and searching and adding relevant articles from the bibliography, and articles that were already known to the authors, sixty studies describing the use of sixty deprivation indices in seventeen countries were included in the narrative synthesis, resulting in development of a taxonomy of indices. When creating the taxonomy, an integrative approach was used that allows integrating new classes and sub-classes in the event that new information appears. Results: In the review, 68% (41/60) of indices were classified as socio-economic, 7% (4/60) of indices as material deprivation, 5% (3/60) of indices as environmental deprivation and 20% (12/60) as multidimensional indices. Conclusions: The data stimulates the use of a competent approach, and will help researchers and public health specialist in resolving conflicts or inconsistencies that arise during the construction and use of indices.
HIV-Associated Systemic Sclerosis: Literature Review and a Rare Case Report
Authors: Hasan Shamimul , Aqil Mohd. , Panigrahi Rajat
Published: August 2022

Highly antiretroviral therapy (HAART) used in Human Immunodeficiency Virus (HIV) treatment may prolong the life span of people living with HIV/Acquired Immune Deficiency Syndrome (AIDS) but may also induce the onset of autoimmune disorders. However, HIV-associated systemic sclerosis (SSc) is an extremely rare occurrence, and only four case reports and two studies documenting this association have been reported to date. We report a rare case of HIV-associated SSc who was referred to us for pain management in her mandibular teeth. A 44-year-old female patient diagnosed with HIV-associated SSc reported a complaint of pain in the lower posterior teeth region. Physical examination revealed typical features of SSc. The pain in her mandibular teeth was due to food lodgement, and she was advised to use toothpaste with a powered toothbrush and mouth stretching exercises, followed by oral prophylaxis. The patient responded well to therapy. HIV-associated SSc is an extremely rare occurrence, with an obscure pathogenic mechanism of HIV-associated autoimmunity. Oral physicians play a crucial role in disease management and should be incorporated into the multidisciplinary team.
Sexual Violence and Substance Use among First-Year University Women: Differences by Sexual Minority Status
Authors: Griffin Kenneth W. , Lindley Lisa L. , Cooper Russell Elaine , Mudd Tori , Williams Christopher , Botvin Gilbert J.
Published: August 2022

Sexual violence and substance use are important public health problems among university students. The present study examined rates of sexual violence victimization, perpetration, and substance use among first-year university women. Participants (n = 974) attending 14 universities across the United States completed an online confidential survey at the beginning and again later in their first year. The sample included women who identified as heterosexual, bisexual, lesbian, and asexual or questioning. The mean age was 19.1 years and 71.4% were White. Rates of victimization involving sexual harassment and sexual acts without consent were higher among sexual minority women relative to heterosexual women, with bisexual women being most likely to report these outcomes. Compared to heterosexual women, sexual minority women reported more frequent cigarette smoking, marijuana use and intoxication, use of club drugs, and overall illicit drug use. Across sexual violence and substance use outcomes, bisexual women reported the highest rates. Sexual minority women reported more accurate beliefs about sexual violence and consent relative to heterosexual women. Over the course of the first year, bisexual women and those who used illicit substances were more likely to report new incidents of sexual violence victimization. Implications for prevention of sexual violence among women, including sexual minorities, are discussed.
Sensitive Detection of Hydroxytyrosol in Extra Virgin Olive Oils with a Novel Biosensor Based on Single-Walled Carbon Nanotubes and Tyrosinase
Authors: Bounegru Alexandra Virginia , Apetrei Constantin
Published: August 2022

Hydroxytyrosol (HT) is an important marker for the authenticity and quality assessment of extra virgin olive oils (EVOO). The aim of the study was the qualitative and quantitative determination of hydroxytyrosol in commercial extra virgin olive oils of different origins and varieties using a newly developed biosensor based on a screen-printed electrode modified with single-layer carbon nanotubes and tyrosinase (SPE-SWCNT-Ty). The enzyme was immobilized on a carbon-based screen-printed electrode previously modified with single-layer carbon nanotubes (SPE-SWCNT-Ty) by the drop-and-dry method, followed by cross-linking with glutaraldehyde. The modified electrode surface was characterized by different methods, including electrochemical (cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS)) and spectrometric (Fourier transform infrared (FTIR) spectroscopy) methods. Cyclic voltammetry was used for the quantitative determination of HT, obtaining a detection limit of 3.49 × 10−8 M and a quantification limit of 1.0 × 10−7 M, with a wide linearity range (0.49–15.602 µM). The electrochemical performance of the SPE-SWCNT-Ty biosensor was compared with that of the modified SPE-SWCNT sensor, and the results showed increased selectivity and sensitivity of the biosensor due to the electrocatalytic activity of tyrosinase. The results obtained from the quantitative determination of HT showed that commercial EVOOs contain significant amounts of HT, proving the high quality of the finished products. The determination of the antiradical activity of HT was carried out spectrophotometrically using the free reagent galvinoxyl. The results showed that there is a very good correlation between the antiradical capacity of EVOOs, the voltammetric response and implicitly the increased concentration of HT. SPE-SWCNT-Ty has multiple advantages such as sensitivity, selectivity, feasibility and low cost and could be used in routine analysis for quality control of food products such as vegetable oils.
Blood Test for Breast Cancer Screening through the Detection of Tumor-Associated Circulating Transcripts
Authors: Park Sunyoung , Ahn Sungwoo , Kim Jee Ye , Kim Jungho , Han Hyun Ju , Hwang Dasom , Park Jungmin , Park Hyung Seok , Park Seho , Kim Gun Min , Sohn Joohyuk , Jeong Joon , Song Yong Uk , Lee Hyeyoung , Kim Seung Il
Published: August 2022

Liquid biopsy has been emerging for early screening and treatment monitoring at each cancer stage. However, the current blood-based diagnostic tools in breast cancer have not been sufficient to understand patient-derived molecular features of aggressive tumors individually. Herein, we aimed to develop a blood test for the early detection of breast cancer with cost-effective and high-throughput considerations in order to combat the challenges associated with precision oncology using mRNA-based tests. We prospectively evaluated 719 blood samples from 404 breast cancer patients and 315 healthy controls, and identified 10 mRNA transcripts whose expression is increased in the blood of breast cancer patients relative to healthy controls. Modeling of the tumor-associated circulating transcripts (TACTs) is performed by means of four different machine learning techniques (artificial neural network (ANN), decision tree (DT), logistic regression (LR), and support vector machine (SVM)). The ANN model had superior sensitivity (90.2%), specificity (80.0%), and accuracy (85.7%) compared with the other three models. Relative to the value of 90.2% achieved using the TACT assay on our test set, the sensitivity values of other conventional assays (mammogram, CEA, and CA 15-3) were comparable or much lower, at 89%, 7%, and 5%, respectively. The sensitivity, specificity, and accuracy of TACTs were appreciably consistent across the different breast cancer stages, suggesting the potential of the TACTs assay as an early diagnosis and prediction of poor outcomes. Our study potentially paves the way for a simple and accurate diagnostic and prognostic tool for liquid biopsy.
Stability of DMPC Liposomes Externally Conjugated with Branched Polyglycerol
Authors: Beketov Alexander A. , Mikhailov Ivan V. , Darinskii Anatoly A.
Published: August 2022

Vesicles formed by DMPC liposomes externally conjugated with branched polyglycerol-dendrons as well as linear PEG in water solution were simulated using the DPD method. Such a structure of vesicles corresponds to the structure of polymer-grafted liposomes obtained experimentally by the post-insertion method, in which polymer chains are fixed on the outer surface of the liposome. The grafting density, generation number and spacer length of grafted dendrons were varied. It was shown that modification of the outer surface of liposomes due to grafting of hydrophilic dendrons has practically no effect on the size and shape of the vesicle, as well as on the morphology of the lipid membrane up to certain critical thresholds of grafting density, degree of polymerization, and generation number of grafted molecules. Exceeding the threshold values of these structural parameters leads to irreversible deformation of the lipid membrane. Diffusion through the membrane and the transition of grafted molecules from the outer surface of the liposome to the inner surface is not observed for dendrons with a generation number higher than one, even at high grafting densities. The critical values of the generation number and the characteristics of the molecular coating at these values were determined for various grafting densities and spacer lengths of the grafted chains. It was shown that the chemical potential of the grafted dendron can serve as a stability metric for the conjugated liposome. The chemical potential of grafted molecules was calculated using the mean field model of the spherical brush on the liposome surface. An analysis of the simulation data shows that, within the framework of the applicability of the mean field approach, the value of the chemical potential is a sufficient criterion for separating vesicles into stable and unstable forms. These results can be used as a guide for the experimental design of nanocontainers based on lipid vesicles with an external protective coating of branched macromolecules.
Essential Acidovorax citrulli Virulence Gene hrpE Activates Host Immune Response against Pathogen
Authors: Ji Weiqin , Zhao Mei , Fei Nuoya , Yang Linlin , Qiao Pei , Walcott Ron , Yang Yuwen , Zhao Tingchang
Published: August 2022

Bacterial fruit blotch (BFB) caused by Acidovorax citrulli (Ac) is a devastating watermelon disease that severely impacts the global watermelon industry. Like other Gram-negative bacteria, the type three secretion system (T3SS) is the main pathogenicity factor of A. citrulli. The T3SS apparatus gene hrpE codes for the Hrp pilus and serves as a conduit to secret effector proteins into host cells. In this study, we found that the deletion of hrpE in A. citrulli results in the loss of pathogenicity on hosts and the hypersensitive response on non-hosts. In addition, the A. citrulli hrpE mutant showed a reduction in in vitro growth, in planta colonization, swimming and twitching motility, and displayed increases in biofilm formation ability compared to the wild type. However, when HrpE was transiently expressed in hosts, the defense responses, including reactive oxygen species bursts, callose deposition, and expression of defense-related genes, were activated. Thus, the A. Citrulli growth in HrpE-pretreated hosts was suppressed. These results indicated that HrpE is essential for A. citrulli virulence but can also be used by hosts to help resist A. citrulli. Our findings provide a better understanding of the T3SS pathogenesis in A. citrulli, thus providing a molecular basis for biopesticide development, and facilitating the effective control of BFB.
Transgenic Expression of Haemonchus contortus Cytochrome P450 Hco-cyp-13A11 Decreases Susceptibility to Particular but Not All Macrocyclic Lactones in the Model Organism Caenorhabditis elegans
Authors: Jakobs Natalie , Yilmaz Esra , Krücken Jürgen
Published: August 2022

The number of reported macrocyclic lactones (ML) resistance cases across all livestock hosts is steadily increasing. Different studies in the parasitic nematode Haemonchus contortus assume the participation of cytochrome P450s (Cyps) enzymes in ML resistance. Still, functional data about their individual contribution to resistance or substrate specificity is missing. Via microinjection, transgenic Caenorhabditis elegans expressing HCON_00141052 (transgene-Hco-cyp-13A11) from extrachromosomal arrays were generated. After 24 h of exposure to different concentrations of ivermectin (IVM), ivermectin aglycone (IVMa), selamectin (SEL), doramectin (DRM), eprinomectin (EPR), and moxidectin (MOX), motility assays were performed to determine the impact of the H. contortus Cyp to the susceptibility of the worms against each ML. While transgene-Hco-cyp-13A11 significantly decreased susceptibility to IVM (four-fold), IVMa (2-fold), and SEL (3-fold), a slight effect for DRM and no effect for MOX, and EPR was observed. This substrate specificity of Hco-cyp-13A11 could not be explained by molecular modeling and docking studies. Hco-Cyp-13A11 molecular models were obtained for alleles from isolates with different resistance statuses. Although 14 amino acid polymorphisms were detected, none was resistance specific. In conclusion, Hco-cyp-13A11 decreased IVM, IVMa, and SEL susceptibility to a different extent, but its potential impact on ML resistance is not driven by polymorphisms.
Development of Hydroxamic Acid Compounds for Inhibition of Metallo-β-Lactamase from Bacillus anthracis
Authors: Huckleby Andrew E. , Saul Jhawn G. , Shin Hyunshun , Desmarais Staci , Bokka Apparao , Jeon Junha , Kim Sung-Kun
Published: August 2022

The emergence of resistant bacteria takes place, endangering the effectiveness of antibiotics. A reason for antibiotic resistance is the presence of lactamases that catalyze the hydrolysis of β-lactam antibiotics. An inhibitor of serine-β-lactamases such as clavulanic acid binds to the active site of the enzymes, thus solving the resistance problem. A pressing issue, however, is that the reaction mechanism of metallo-β-lactamases (MBLs) hydrolyzing β-lactam antibiotics differs from that of serine-β-lactamases due to the existence of zinc ions in the active site of MBLs. Thus, the development of potential inhibitors for MBLs remains urgent. Here, the ability to inhibit MBL from Bacillus anthracis (Bla2) was investigated in silico and in vitro using compounds possessing two hydroxamate functional groups such as 3-chloro-N-hydroxy-4-(7-(hydroxyamino)-7-oxoheptyl)benzamide (Compound 4) and N-hydroxy-4-(7-(hydroxyamino)-7-oxoheptyl)-3-methoxybenzamide (Compound 6). In silico docking and molecular dynamics simulations revealed that both Compounds 4 and 6 were coordinated with zinc ions in the active site, suggesting that the hydroxamate group attached to the aromatic ring of the compound plays a crucial role in the coordination to the zinc ions. In vitro kinetic analysis demonstrated that the mode of inhibitions for Compounds 4 and 6 were a competitive inhibition with Ki values of 6.4 ± 1.7 and 4.7 ± 1.4 kcal/mol, respectively. The agreement between in silico and in vitro investigations indicates that compounds containing dihyroxamate moieties may offer a new avenue to overcome antibiotic resistance to bacteria.