bluuebunny/crossref_metadata_2025 huggingface dataset:
KDM3B inhibitors disrupt PAX3-FOXO1 oncogenic activity in fusion positive rhabdomyosarcoma.
Authors: Kim Yong Yean , Gryder Berkley , Sinniah Ranuka , Peach Megan , Shern Jack , Abdelmaksoud Abdalla , Pomella Silvia , Woldemichael Girma , Stanton Benjamin , Milewski David , Barchi Joe , Schneekloth John , Chari Raj , Kowalczyk Joshua , Shenoy Shilpa , Evans Jason , Song Young , Wang Chaoyu , Wen Xinyu , Chou Hsien-chao , Gangalapudi Vineela , Esposito Dominic , Jones Jane , Procter Lauren , O’Neill Maura , Jenkins Lisa , Wei Jun , McMahon James , O'Keefe Barry , Hawley Robert , Khan Javed
Published: November 2022

Fusion-positive alveolar rhabdomyosarcoma (FP-RMS) is an aggressive pediatric sarcoma driven primarily by the PAX3-FOXO1 fusion oncogene, for which therapies targeting PAX3-FOXO1 are lacking. We screened 62,643 compounds using an engineered cell line that monitors PAX3-FOXO1 transcriptional activity identifying a hitherto uncharacterized compound, PFI-63. RNA-seq, ATAC-seq, and docking analyses implicated histone lysine demethylases (KDMs) as its targets. Enzymatic assays confirmed the inhibition of multiple KDMs with highest selectivity for KDM3B. Structural similarity search of PFI-63 identified PFI-90 with improved solubility and potency. Biophysical binding of PFI-90 to KDM3B was demonstrated using NMR and SPR. PFI-90 suppressed the growth of FP-RMS in vitro and in vivo through downregulating PAX3-FOXO1 activity, and combined knockdown of KDM3B and KDM1A phenocopied PFI-90 effects. Thus, we report novel KDM inhibitors with highest specificity for KDM3B. Its potent suppression of PAX3-FOXO1 activity can be exploited as a new therapeutic approach for FP-RMS and other transcriptionally driven cancers.
The use of artificial intelligence models to predict survival in patients with laryngeal squamous cell carcinoma
Authors: Choi Nayeon , Kim Junghyun , Yi Heejun , Kim HeeJung , Kim Tae Hwan , Chung Myung Jin , Ji Migyeong , Lee Sujeong , Kim Zero , Son Young-Ik
Published: November 2022

Most recent survival prediction has been based on TNM staging, which does not provide individualized information. However, clinical factors including performance status, age, sex, and smoking might influence survival. Therefore, we used artificial intelligence (AI) to analyze various clinical factors to precisely predict the survival of patients with larynx squamous cell carcinoma (LSCC). We included patients with LSCC (N=1026) who received definitive treatment from 2002 to 2020. Age, sex, smoking, alcohol consumption, Eastern Cooperative Oncology Group (ECOG) performance status, location of tumor, TNM stage, and treatment methods were analyzed using deep neural network (DNN) with multi-classification and regression, random survival forest (RSF), and Cox proportional hazards (COX-PH) model for prediction of overall survival. Each model was confirmed with five-fold cross validation, and performance was evaluated using linear slope, y-intercept, and C-index. The DNN with multi-classification model demonstrated the highest prediction power (1.000 ± 0.047, 0.126 ± 0.762, and 0.859 ± 0.018 for slope, y-intercept, and C-index, respectively), and the prediction survival curve showed the strongest agreement with the validation survival curve, followed by DNN with regression (0.731 ± 0.048, 9.659 ± 0.964, and 0.893 ± 0.017, respectively). The DNN model produced with only T/N staging showed the poorest survival prediction. When predicting the survival of LSCC patients, various clinical factors should be considered. In the present study, DNN with multi-class was shown to be an appropriate method for survival prediction. AI analysis may predict survival more accurately and improve oncologic outcomes.
The potential role of intestinal dysbacteriosis-related genes in hypertension: a bridge for future targeted therapies
Authors: wang kuangyi , jiang yufeng , Chen Tan , Zhou Yafeng
Published: November 2022

Background: Changes in the balance of intestinal flora can cause many diseases. This article offers a new direction to the future therapy of hypertension by screening potential drug targets for hypertension related to intestinal flora.
Method: Differential expression of GSE74144 in Gene Expression Omnibus (GEO) database was analyzed by GEO2R, grouping based on whether they had hypertension or not. Intestinal flora-related genes retrieved from the GeneCards database were intersected with the differential genes. The protein-protein interaction network was then constructed and the central gene was identified by Cytoscape. Candidate pharmacological compounds for differentially expressed genes were filtrated by cMap.
Results: We found high-expressed genes and 12 low-expressed genes were found in hypertension and intestinal microbiota related genes. These genes participate in biological processes including immune response regulation, bacterial defense response, glycolysis, viral response, etc. KEGG results show that these genes are related to inflammatory bowel disease, immune cell differentiation.
Conclusions: Through bioinformatics analysis, we have revealed the key genes related to intestinal flora in hypertension, as well as 10 drugs that may be used to treat hypertension, which provides a new way to treat hypertension in the future.
Emotional psychopaths? Differentiation of various emotional states experienced by psychopathic individuals
Authors: Bolek Ewelina , Trzebińska Ewa , Braniecka Anna
Published: November 2022

The study examined experience of different forms of emotion by people exhibiting strong psychopathic traits and to compare them in this regard with non-psychopathic individuals. One hundred and twenty-six individuals took part in a computer experiment in which they were shown six excerpts from the film evoking various forms of emotions: (1) negative emotion, (2) positive emotion, (3) simultaneously mixed emotion, (4) sequentially mixed emotion (positive following negative), (5) secondary emotion mixed, (6) lack of emotion. Subsequently they reported (1) the intensity of the emotions experienced, (2) the tension and (3) ambivalence associated with them. The severity of psychopathic traits was measured using the Self-Report Psychopathy Scale-III. Results revealed that people with stronger psychopathic traits experienced these particular forms of emotions in a different way to others. The differences are related to negative emotions and sequentially mixed emotions. Negative emotions were experienced by psychopathic people at greater intensity and, accompanied by greater tension. In addition, two aspects of psychopathy: criminal tendencies in its less severe form and interpersonal manipulation were shown to be moderators for these differences, confirming previous findings that psychological traits are the primary mechanisms of psychopathy, rather than antisocial behavior itself.
Rapid Determination of 61 Acid Industrial Dyes in Chili Patse, Hotpot Seasoning, Bearnaise Using Double-Liquid Liquid Extraction and Ultrahigh-performance Liquid Chromatography Quadrupole Orbitrap High-resolution Mass Spectrometry
Authors: Li Shuang , Meng Yanan , Zhang Shun , Cai Ting
Published: November 2022

A rapid screening method of 61 acid dyes in complex food matrix such as chili patse, hotpot seasoning, bearnaise was proposed by using double liquid-liquid extraction (d-LLE), which can maximum remove interference from lipid-soluble compounds and highly hydrophilic compounds. The extract was analyzed by ultrahigh-performance liquid chromatography quadrupole orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap-HRMS). The preliminary qualitative analysis was performed by extracting characteristic ion fragments (m/z 79.9557) based on in-source collision induced dissociation (CID) in full scan MS. Linearity was observed in the range of 0.01 ~ 0.2 µg/mL and the limits of quantification were 0.05 mg/kg for 61 acid dyes. The average recoveries were 75.2% ~ 95.3%, with RSD ≤ 10%. The proposed method has the ability to rapid screen acid dyes in complex foods with high economy, sensitivity and reliability.
Electrical approach for decontaminating a multi-metal polluted soil as influenced by electrolyte type and soil position
Authors: Keshavarz Samira , Ghasemi-Fasaei Reza , Ronaghi Abdolmajid , Mousavi Ali Akbar
Published: November 2022

The remediation of heavy metals contaminated soils is increasingly a global problem with serious implications for human health. This study aimed to evaluate the in-situ remediation performance of multi element contaminated soil by the electrokinetic. To achieve this, the effects of chelating agents (water, ethylenediamine tetraacetic acid (EDTA), diethylenetriamine pentaacetic acid (DTPA)), potential gradient (0, 1, and 2 V cm− 1), and position of soil in electrokinetic cell on metals fractions and metals removal were investigated. The results revealed that the electric potential difference and application of EDTA or DTPA electrolyte generally enhanced heavy metals removal efficiency and for Ni and Pb the interactions effects of these factors were significantly positive. Results showed that Ni, Zn, Cd, and Pb removal efficiency is highest with DTPA, DTPA, EDTA and EDTA electrolytes, respectively. In particular, the usage of electric current remarkably shifted the soil-metal bonds from stable (residual) to a less stable (mobile and mobilisable) fraction. The optimum electric current for the removal of Zn, Cd, and Ni was 1, 1, and 2 V cm− 1, respectively, which removed 44, 47 and 41% of the average of these heavy metals in soil, respectively. Results of present study demonstrated that removal efficiency was highly metal-dependent; and the order of metals removal was Cd > Ni > Zn > Pb.
Using multi-criteria decision analysis to describe stakeholder preferences for new quality improvement initiatives that could optimise prescribing in England: A case study
Authors: Khanal Saval , Schmidtke Kelly Ann , Talat Usman , Turner Alice M , Vlaev Ivo
Published: November 2022

Background

Hospital decision-makers have limited resources to implement quality improvement projects. To decide which interventions to take forward, trade-offs must be considered that inevitably turn on stakeholder preferences. The multi-criteria decision analysis (MCDA) approach could make this decision process more transparent.

Method

An MCDA was conducted to rank-order four types of interventions that could optimise medication use in England’s National Healthcare System (NHS) hospitals, including Computerised Interface, Built Environment, Written Communication, and Face-to-Face Interactions. Initially, a core group of quality improvers (N = 10) was convened to determine criteria that could influence which interventions are taken forward according to the Consolidated Framework for Implementation Research. Next, to determine preference weightings, a preference survey was conducted with a diverse group of quality improvers (N = 356) according to the Potentially All Pairwise Ranking of All Possible Alternatives method. Then, rank orders of four intervention types were calculated according to models with criteria unweighted and weighted according to participant preferences.

Results

The most important criteria influencing what interventions were preferred was whether they addressed ‘patient needs’ and their financial ‘cost’. In both the unweighted and weighted models, the top-ranked intervention type was Computerised Interface followed by Built Environment. In the unweighted model, Written Communication and Face-to-Face intervention types were ranked similarly, while in the weighted model the Face-to-Face type was ranked lower.

Conclusions

An MCDA was conducted to rank order intervention types that stand to increase medication optimisation across hospitals in England. The top-ranked intervention type was Computerised Interface. This finding does not imply Computerised Interface interventions are the most effective interventions but suggest that successfully implementing lower-ranked interventions may require more conversations to appreciate stakeholder concerns.
On-line Supercapacitor State-of-Charge Diagnosis in Electric Vehicle Application
Authors: Moosavi S. S. , Moghadasian M. , Golpour M.
Published: November 2022

The authors have requested that this preprint be removed from Research Square.
Multiscale reconstruction of bronchus and cancer cells in human stage II lung adenocarcinoma
Authors: Li Xin , Zhang Bowen , Liang Yanmei , Li Ting
Published: November 2022

Background
While previous studies primarily focused on the structure of the normal whole mouse lung, the whole bronchus and cytoarchitectural details of the mouse intact lung lobe have been discovered at single-cell resolution. Revealing the sophisticated lung adenocarcinoma structure at three-dimensional (3D) and single-cell level remains a fundamental and critical challenge for the pathological mechanism research of lung adenocarcinoma (LA).
Methods
Fluorescence micro-optical Sectioning Tomography (fMOST) combined with PI staining were used to obtain the 3D imaging of the human LA tissues at single-cell resolution.
Results
With a spatial resolution of 0.32 × 0.32 × 1.0 µm3, the dataset of human LA with single-cell precision consists of two channels, each of which contains information about the bronchi and the cytoarchitecture. The bronchial wall is thicker and the lumen is smaller in the adenocarcinoma tissue, in which its original normal structure is vanished. More solid components, more clustered cancer cells with larger nucleoli, and more significant atypia are found in cancer tissues. In paracancerous tissues, the bronchial wall cells have a monolayer or bilayer structure, cluster along the wall, and are relatively dispersed. Few fibrous structures and occasional dissemination of spread through air spaces (STAS) are observed.
Conclusions
Based on the human lung adenocarcinoma tissue database obtained by fMOST and PI staining, the bronchi and cells were reconstructed and visualized. This work provides a technical roadmap for studying the bronchus and cytoarchitectural structure and their spatial relationship in lung adenocarcinoma tissue, which will help with the accurate diagnosis and assessment of the medical effectiveness of lung adenocarcinoma in the future.
Understanding daily rhythms in weakly electric fish: the role of melatonin on the electric behavior of Brachyhypopomus gauderio
Authors: Vazquez Juan I. , Gascue Valentina , Quintana Laura , Migliaro Adriana
Published: November 2022

Living organisms display an array of molecular, physiological and behavioral rhythms synchronized with natural environmental cycles. Understanding these interactions gains power when the complexity of natural habitats and the diversity of behavioral and physiological adaptations are taken into account. Brachyhypopomus gauderio, a South American weakly electric fish, are characterized by the emission of electrical discharges (EOD), with a very stable rate that is modulated by social and environmental cues. The nocturnal arousal in B. gauderio coincides with a melatonin-dependent EOD rate increase. Here we first show a daily cycle in both the EOD-BR and EOD-BR variability of B. gauderio in nature. We approached the understanding of the role of melatonin on this natural behavior through both behavioral pharmacology and in vitro assays. We report, for the first time in gymnotiformes, a direct effect of melatonin on the PN in vitro preparation. Melatonin treatment lowered EOD-BR in freely moving fish and PN-BR, while increasing the variability of both. These results show that melatonin plays a key role in modulating the electric behavior of B. gauderio through its effect on rate and variability, both of which must be under a tight temporal regulation to prepare the animal for a challenging nocturnal environment.
Hyperproduction of extracellular polymeric substance in Pseudomonas fluorescens for efficient Chromium (VI) absorption
Authors: Yang Lijie , Chen Zhen , Zhang Ying , Lu Fuping , Liu Yihan , Cao Mingfeng , He Ning
Published: November 2022

A novel extracellular polymeric substance (EPS) with flocculating activity produced by Pseudomonas fluorescein isolated from soil was studied in this paper. Firstly, atmospheric and room temperature plasma (ARTP) was applied to get a mutant of P. fluorescein with higher EPS production. A mutant T4-2 exhibited a 106.48% increase in flocculating activity compared to the original strain. The maximum EPS yield from T4-2 was enhanced up to 6.42 g/L, nearly 10 times higher than the original strain on a 3.6-L fermentor with optimized fermentation conditions. Moreover, the flocculating activity of the mutant reached 3023.4 U/mL, 10.96-fold higher than that of T4. Further identification showed that EPS from T4-2 was mainly composed of polysaccharide (76.67%) and protein (15.8%) with a molecular weight of 1.17×105 Da. The EPS showed excellent adsorption capacities of 80.13 mg/g for chromium (Ⅵ), which was much higher than many reported adsorbents such as chitosan and cellulose. The adsorption results were described by Langmuir isotherm and pseudo-second order kinetic model. The thermodynamic parameters (ΔG0, ΔH0 and ΔS0) revealed that the adsorption process was spontaneous and exothermic. Adsorption mechanisms were speculated to be electrostatic interaction, reduction, and chelation.
Acculturation stress and post-traumatic growth among female North Korean defectors: A moderated mediation effect of loneliness and neighborhood social cohesion
Authors: Kim Hokon , Kim Ocksim , Kim Kyung-A , Chu Sang Hui , Chung Misook L.
Published: November 2022

Purpose
Extant literature shows that loneliness negatively predicts post-traumatic growth (PTG) among North Korean defectors (NKD). Female NKD are more vulnerable to various traumatic experience in the entire process from escaping North Korea and to entering South Korea. Evidence also suggests that the experience of migration and settlement to South Korea causes acculturation stress and loneliness and could be affected by neighborhood social cohesion. This study explores the mediating effect of loneliness on relationship between acculturation stress and PTG, and the moderating effect of neighborhood social cohesion on the relationship between acculturation stress and loneliness among female NKD.
Methods
Instruments used were the short-form of the UCLA Loneliness Scale, Post-Traumatic Growth inventory, part of the Neighborhood-level Cohesion and Disorder scale, and the revised Acculturation Stress Scale for International Students for NKD. Data were analyzed from 166 females NKD who participated in an online survey from July to August 2020. Statistical analysis, including mediating and moderating effect analysis, was performed using SPSS 26.0 and PROCESS 4.0.
Results
Loneliness negatively impacted PTG (B=-2.118, p < 0.001), and loneliness mediated the relationship between acculturation stress and post-traumatic growth (B=-0.294, 95% LLCI=-.415, 95% ULCI=-0.186). High neighborhood social cohesion decreased loneliness, and neighborhood social cohesion moderated the relationship between acculturation stress and loneliness (B=-0.018, 95% LLCI=,0.001 95% ULCI = 0.152).
Conclusion
Increasing neighborhood social cohesion in female NKD would reduce loneliness caused by acculturation stress and help their positive growth. This represents the most appropriate approach to help female NKD achieve growth, even after suffering trauma.
Syntheses, Crystal Structure and Fluorescence Properties of a Cd (Ⅱ) coordination polymer based on pyridinium zwitterionic ligand
Authors: Wang Kaimin , Dong Yanqiu , Zhao Xiong , Bai Xuling , Duan Keyan , Ye Yanqing , Guo Jinrong , Wang Zhengliang , Tang Huaijun , Ma Yulu
Published: November 2022

With pyridinium zwitterionic ligand H2LCl2 (1,1'-((2,3,5,6-tetramethyl-1,4-phenylene)bis(methylene))bis(4-carboxypyridin-1-ium)chloride) as the main connection point, a novel Cd (Ⅱ) coordination polymer namely [Cd(L)2(SO4)2(H2O)2][Cd(H2O)6]·4H2O (CP 1) and a partially deprotonated HLClO4·H2O were obtained by room temperature volatilization method. X-ray diffraction studies reveal that HLClO4·H2O crystallizes in the monoclinic P21/n space group, with a bowl shape partially deprotonated HL+ cation as the main structure, and the ClO4-anion which balances the charge just exists outside. When the ligand reacts with CdSO4‧8/3H2O, CP 1 crystallized in the triclinic P-1 space group with two distinct Cd (Ⅱ) centers was obtained. Abundant hydrogen bonds produce the 3D supramolecular structure of CP 1. Elemental analysis, powder X-ray diffraction, infrared spectrum, thermogravimetric analysis, solid-state fluorescence properties and CIE analysis of CP 1 were studied. Results indicate that the CP 1 has good thermal stability and has potential applications in blue luminescent materials.
EIF2S3 is a Prognostic Biomarker Correlated With Immune Infiltration in Hepatocellular Carcinoma
Authors: chen zhonghuo , Wang Wen-Jun , Tang Lei , Wei Chao , Wang Ben , Xiao Han , Tao Xiao-fei , Yang Jia-Le , Yu Tao-Zhi , Guan Jia-Fu , Yuan Rong-Fa , Yu Xin , Hu Zhi-Gang , Wu Hua-Jun , Wang Kai
Published: November 2022

Background: Hepatocellular carcinoma (HCC) is one of the most frequent cancers with a high recurrence rate and poor prognosis. eukaryotic translation initiation factor 2 subunit gamma (EIF2S3) is a heterotrimeric GTP binding protein, taking part in the recruitment of methionyl-tRNA to the 40 S ribosomal subunit. However, the role of EIF2S3 in HCC remains unclear.
Materials and methods: In the present study, the expression level of EIF2S3 in pan-cancer was identified using TIMER database and its level in HCC tissues was detected by TCGA database. Univariate and multivariate Cox regression analysis was performed to evaluate the relationship between EIF2S3 and clinical characteristics in HCC. Kaplan-Meier Plotter analysis was used to assess the prognostic role of EIF2S3 in HCC patients. Function enrichment analysis was conducted by LinkedOmics database. The association between EIF2S3 level and immune infiltration, and the correlation between EIF2S3 expression and immune cell biomarkers in HCC were analyzed by TIMER and GEPIA database. Finally, the protein expression of EIF2S3 in HCC cell lines was analyzed and the proliferation, migration, invasion and apoptosis of HCC cells after silencing EIF2S3, which were detected by cell viability assay, wound healing assay, transwell assay and flow cytometry analysis, respectively.
Results: EIF2S3 was overexpressed in many solid tumors. And, high EIF2S3 expression was detected in HCC and significantly related to tumor status and prognosis of HCC patients. Moreover, EIF2S3 expression was positively related to immune cell infiltration and gene marker of immune cells in HCC patients. Cell experiments showed thatEIF2S3 was overexpressed in HCC cells and EIF2S3 enhanced the proliferation, migration and invasion of HCC cells and decreased the apoptosis of HCC cells.
Conclusion: EIF2S3 may be involved in the development of HCC and could be a prognostic genomic marker and a novel therapeutic target for HCC.
Efficacy of Intrathecal Methotrexate in Children With High-risk Medulloblastoma over 3 years: A retrospective study from a single center
Authors: Zhang YuTong , Wang Yu , Zhang Chao , Zhong Xiaodan , Chang Jian
Published: November 2022

Purpose To evaluate the therapeutic benefits and side effects (especially leukoencephalopathy) of intrathecal methotrexate (MTX) in children aged over three years old with high-risk medulloblastoma (MB).
Patients and Methods In the present retrospective study, patients who received intrathecal MTX during chemotherapy were classified as MTX group, while those receiving cerebrospinal fluid (CSF) cytology analysis only were recruited in control group.
Results Among the 46 MB patients, 32 were classified in MTX group, whereas 14 in control group. For those 32 patients in MTX group, 27 (84.38%) had metastatic disease, 3 (9.38%) had diffuse anaplasia, and 3 (9.38%) had residual disease greater than 1.5 cm2. Molecular subgroup classification was available in 28 (87.5%) patients. Of those 14 patients in control group, 8 (57.14%) had metastatic disease, 3 (27.27%) had diffuse anaplasia, and 6 (42.86%) had residual disease greater than 1.5 cm2. Molecular subgroup classification was available in 6 (42.86%) patients. The 5-year progression-free survival (PFS) was 70.99% (95% CI, 58.22%-90.42%) and 5-year overall survival (OS) was 72.99% (95% CI, 60.41%-93.06%) for MTX group, while those were 41.67% (95% CI, 17.93%-66.92%) and 50% (95% CI, 27.88%-77.14%) for control group, separately. Among the 32 patients in MTX group, 6 (18.75%) with group 4 disease developed MTX-related acute leukoencephalopathy and one of them died.
Conclusions Intrathecal MTX improves the 5-year PFS and OS for children with high-risk MB. However, intrathecal MTX is not recommended for patients with group 4 MB due to the high risk of acute leukoencephalopathy.
Galvanic displacement of Co with Rh boosts hydrogen and oxygen evolution reactions in alkaline media
Authors: Vasiljević Bojana Nedić , Jovanović Aleksandar Z. , Mentus Slavko V. , Skorodumova Natalia V. , Pasti Igor
Published: November 2022

The growing energy crisis put an emphasis on the development of novel efficient energy conversion and storage systems. Here we show that surface modification of cobalt by a fast galvanic displacement with rhodium significantly affects the activity towards hydrogen (HER) and oxygen evolution (OER) in alkaline media. After only 20 seconds of galvanic displacement, the HER overpotential is reduced by 0.16 V and OER overpotential by 0.06 V. This reduces the predicted water splitting voltage from 2.03 V (clean Co anode and cathode) to 1.81 V at 10 mA cm− 2. During the galvanic displacement process, the surface roughness of the Co electrode does not suffer significant changes, which suggests an increase in the intrinsic catalytic activity. Density Functional Theory calculations suggest that the reactivity of the Rh-modified Co(0001) surface is modified compared to the clean one. In the case of HER, experimentally observed activity improvements are directly correlated to the weakening of the hydrogen-surface bond, confirming the beneficial role of Rh incorporation into the Co surface.
SMARCA4-deficient dedifferentiated endometrioid carcinoma
Authors: Huang Ruihong , Chen Lvxuan , Pan Canyu , Zhang Peiru , Fang Xueting , Yi Jingyi
Published: November 2022

Background
SMARCA4-deficient, dedifferentiated endometrioid carcinoma （SDDEC）with distinct rhabdoid cells is a rare uterine malignancy. A 58-year-old case was presented.The undifferentiated carcinoma component was 90% and filled with rhabdoid cells, which is easily mistaken for SMARCA4-deficient undifferentiated uterine sarcoma (SDUS). In contrast to earlier findings, the undifferentiated carcinoma component's MMR status was distinct from that of endometrioid adenocarcinoma. We discuss the clinicopathological characteristics, the clinical course of treatment, and a literature review.
Case presentation
The patient was 58 years old and admitted with postmenopausal vaginal bleeding and lower abdominal pain. An MRI revealed a significant uterine tumor and an ambiguous sigmoid colon boundary. A cytoreductive procedure was carried out. A CT scan performed two weeks after surgery revealed liver metastases and new, enlarged lymph nodes in the mediastinum. Anti-PD-1 immunotherapy was tested. One month after the procedure, the patient died. The final pathological diagnosis was SMARCA4-deficient dedifferentiated endometrial carcinoma. SMARCA4 loss was only present in the undifferentiated carcinoma, which manifested as MMRp. As well, the endometrioid adenocarcinoma component had intact SMARCB1 and SMARCA4 expression, which manifested as MMRd.
Conclusion
Mutations in SMARCA4 result in clinically hyperaggressive malignant rhabdomyoblastic tumors.Future research will focus heavily on multimodal therapy, which combines surgical tumor removal, postoperative chemotherapy, radiation, and immunotherapy, for SDDEC.
Clinical characteristics and prognosis of connective tissue disease-associated pulmonary arterial hypertension
Authors: Li Meiqi , Zhang Qingfeng , Xiang Weizhen , Dong Rongrong , Yan Suyan , Ma Zhenzhen , Yang Qingrui
Published: November 2022

Background
Pulmonary arterial hypertension (PAH) is a series of clinicopathological syndromes associated with elevated pulmonary arterial pressure caused by various reasons, and is also the most serious complication of connective tissue disease (CTD). This study aims to evaluate the clinical features and prognostic factors of CTD associated PAH (CTD-PAH) patients in the Chinese Han population, and provide a reference basis for improving their prognosis.
Methods
A total of 392 individuals with CTD between January 2017 and August 2021 were included in this study. They were divided into two main groups: CTD-PAH and CTD-non-PAH. The independent sample t-test and Mann-Whitney U test were used to compare clinical and laboratory findings. Receiver operating characteristic (ROC) curves were converted into binary variables to determine the critical value. A Kaplan-Meier survival analysis was used to compare the survival rates of the two groups. And an analysis of death risk factors was conducted using Cox regression.
Results
In this study, SLE was the most common underlying disease among Chinese Han patients with CTD-PAH (51.56%). The level of anti-U1-RNP antibodies was significantly higher in the CTD-PAH group as compared to the CTD-non-PAH group (P = 0.001), whereas the levels of anti-SCL-70 antibodies were lower (P < 0.001). CTD patients with higher anti-U1-RNP antibodies levels were at high risk for PAH (P = 0.021), whereas higher levels of anti-U1-RNP have also been linked to decreased mortality in CTD-PAH (P < 0.001). In addition, the anti-SCL-70 antibody was an independent protective factor for CTD-PAH (P = 0.029). The poor prognosis of CTD-PAH was associated with high RDW (P = 0.010), NLR (P < 0.001), CAR (P = 0.001) and pulmonary infection (P = 0.008).
Conclusion
Patients with CTD who have higher levels of anti-U1-RNP antibodies are at high risk for PAH, whereas higher levels of anti-U1-RNP antibodies have also been linked to decreased mortality. An increase in the anti-SCL-70 antibody levels is an independent protective factor against CTD-PAH. High CAR, NLR, RDW and pulmonary infection are also significant determinants of total mortality in patients with CTD-PAH.
Performance and Economic Efficiency of Bovans Brown Laying Hens Supplemented With Effective Microorganisms
Authors: Yitbarek Melkamu Bezabih
Published: November 2022

Background
Effective Microorganisms is the mixed cell culture composed of photosynthetic bacteria, actinomyces, yeast, lactobacillus and fungi which improves growth rate, feed efficiency, egg production and increases the health status of poultry. The experiment was conducted to evaluate the economic efficiency of effective microorganism’s supplementation to Bovans Brown laying hens.
Results
The result revealed that layers fed in T4 (124.07g) was significantly higher (P < 0.05) than T1 (119.67g), T2 (122.36g) and T3 (122.37g). The total egg production during the experimental period (week 20 to week 67) was 241,242,249 and 268 for T1, T2, T3 and T4, respectively. The highest (P < 0.05) feed conversion ratio was observed in T4 (2.23). The total feed cost was T1 > T2 > T3 > T4. The highest net revenue was obtained from T4 and the lowest was T1 and T2. The economic efficiency was ranged from 0.66 to 0.90 and the feed cost ratio was ranged from 1.66 to 1.90 ETB.
Conclusion
Therefore, from this study it can be concluded that supplementation of EM with drinking water and in feed (T4) had the best production performance indices among the treatment groups.
A novel systemic inflammation response index (SIRI) for predicting postoperative survival of patients with endometrial cancer
Authors: Tu Yuan , Jiang Peng , Wang Jinyu , Huang Yuzhen , Kong Wei , Li Ning , Zheng Yunfeng , Zhou Qian , Gou Shikai , Tian Chenfan , Yuan Rui
Published: November 2022

Background
Systemic Inflammation Response Index (SIRI) has been reported as an effective biomarker to predict prognosis in various cancers. However, the prognostic value of SIRI in endometrial cancer remains unclear. This study aimed to investigate the potential prognostic significance of SIRI in patients with endometrial cancer.
Methods
A total of 586 patients with endometrial cancer who underwent radical surgical treatment was retrospectively recruited from the First Affiliated Hospital of Chongqing Medical University. Univariate and multivariate analysis were used to select parameters related to recurrence of patients with endometrial cancer. The optimal threshold of SIRI was assessed by receiver operating characteristic curve. Patients were divided into low-SIRI and high-SIRI groups according to the optimal SIRI cut-off value. The differences of clinicopathological parameters and survival analysis between the low-SIRI and high-SIRI groups were compared.
Results
Multivariate analysis indicated that SIRI was an independent predictor for recurrence and death of endometrial cancer. The optimal threshold of SIRI for predicting recurrence and death of endometrial cancer was 1.29. Endometrial cancer patients in low-SIRI group had better prognosis than those in the high-SIRI group (P < 0.001). Among the 231 patients without adjuvant therapy, patients in the low-SIRI group had longer recurrence-free survival and overall survival than those in the high- SIRI group (P = 0.004, P = 0.003).
Conclusions
SIRI was identified as an independent prognostic factor for endometrial cancer. Endometrial cancer patients with higher SIRI had a worse prognosis. High-SIRI could be a prognostic stratification factor for endometrial patients.
Effects of different water quality regulators on growth performance, immunologic function and domestic water quality of GIFT tilapia (Oreochromis niloticus, GIFT strain)
Authors: Wang Liang-Gang , Liu Meng-Qian , Xie Xiao-Dong , Sun Yu-Bo , Zhang Ming-Lin , Zhao Yi , Chen Qi , Ding Yi-Qu , Yu Mei-Ling , Liang Zheng-Min , Hu Ting-Jun , Liang Wan-Wen , Wei Ying-Yi
Published: November 2022

Potassium monopersulfate (KMPS), tetrakis hydroxymethyl phosphonium sulfate (THPS), bacillus subtilis (BS) and chitosan (CS) are four new types of water quality regulators, which play an important role in aquaculture. In the 35-day Oreochromis niloticus (GIFT tilapia) feeding trial, all four drugs could slow down the increasing rate of ammonia nitrogen, nitrite, active phosphate and harmful substances of water in the process of GIFT tilapia culture. At the same time, the growth performance, organ index, blood physiology and biochemistry were improved, acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase activity (SOD) and total antioxidant capacity(T-AOC) of serum were increased, and feed coefficient (CF) was reduced. In KMPS and THPS groups, the contents of glutamic oxaloacetic transaminase (AST), glutamic pyruvic transaminase (ALT) and creatinine (Cr) of serum were significantly increased, and the hepato-somatic index (HSI) was significantly decreased during the whole feeding process. In KMPS and THPS groups, the histomorphology of liver showed obvious pathological changes. The results showed that KMPS, THPS, BS and CS could effectively improve the water quality, growth performance and immune function of GIFT tilapia. Nevertheless, frequent use of KMPS and THPS will adversely affects organ and tissue of GIFT tilapia.
The efficacy and safety of radiofrequency ablation in the treatment of inoperable patients with pulmonary malignant nodules
Authors: Qie Peng , Xun Xuejiao , Nie Xiaodong , Yin Qifan , Cui Hongshang , Liu Lijun , Wang Huien
Published: November 2022

Objective: Radiofrequency ablation(RFA) has been recently applied as an alternative treatment in the patients with pulmonary malignancies. The aim of our study was to assess the incidence of complications and survival rate of RFA for malignant lung nodules, and evaluate the efficacy and safety of RFA in the treatment of inoperable patients with pulmonary malignant nodules.
Methods: The clinical data of 50 patients (34 men and 16 women) aged 74.2(range 65-84) years with primary(n=42) and metastatic(n=8) lung malignant nodules treated with RFA from June 2015 and July 2017 in Hebei General Hospital were considered for this study, and the characteristics and clinical data of these patients were analyzed. Complications, progression-free survival, and overall survival at 1, 2 and 5 years of these patients were evaluated.
Results: Following the procedure. There were no major complications and deaths during the operation. 26(52%) patients presented mild-to-moderate chest pain that was easily controlled by analgesic drugs. 8(16%) patients with pneumothorax, 4(8%) haemoptysis, 6(12%) pneumonia, 7(14%) pleural effusion, and 1(2%) postoperative bronchopleural fistula. Needle-track implantation was observed in 2(4%) patients. Median progression-free survival(PFS) was 24.6 months(range, 6.8-60 months). The PFS at 1, 2, 5 years was 76%, 52%, and 20% respectively. Median overall survival(OS) was 35.5 months (range 10.2-60 months). The OS at 1, 2, and 5 years was 80%, 58%, and 32%, respectively.
Conclusion: RFA is a safe and effective alternative treatment for the inoperable patients with primary or metastatic pulmonary malignant nodules. The clinical impact and long-term results of RFA need to be further confirmed in a larger series of patients, and RFA should ideally be compared with surgery.
Prescription Pattern Analysis of Type 2 Diabetes Mellitus: A Cross-Sectional Study in Isfahan, Iran
Authors: Ziad Elnaz , Malekpour Mohammad-Reza , Farzadfar Farshad , Sadat Somayeh
Published: November 2022

Background
Patients with Type 2 Diabetes Mellitus (T2DM) are at a higher risk of polypharmacy and more susceptible to irrational prescriptions; therefore, pharmacological therapy patterns are important to be monitored. The primary objective of this study was to highlight the current prescription patterns in TD2M patients and compare them with the existing standards of medical care in diabetes. The second objective was to analyze whether age and gender affect prescription patterns.
Methods
This cross-sectional study was conducted using Iran Health Insurance Organization (IHIO) prescription database. It was mined by an Association Rule Mining (ARM) technique, named FP-Growth, in order to find co-prescribed drugs with anti-diabetic medications. The algorithm was implemented on different levels of Anatomical Therapeutic Chemical (ATC) classification system, which assigns different codes to drugs based on their anatomy, pharmacological, therapeutics and chemical properties, to provide in-depth analysis of co-prescription patterns.
Results
Altogether the prescriptions of 914,652 patients, out of which 91,505 were diabetic, were analyzed. According to our results, prescribing Lipid Modifying Agents (C10) (56.3%), Agents Acting on The Renin-Angiotensin System (C09) (48.9%), Antithrombotic Agents (B01) (35.7%), and Beta Blocking Agents (C07) (30.1%) were meaningfully associated with the prescription of Drugs Used in Diabetes. Our study also revealed that female diabetic patients have a higher chance of taking Antithyroid agents, and the older the patients were, the more they were prone to take neuropathy-related medications.
Conclusions
Almost all of the association rules found in this research were clinically meaningful, proving the potential of ARM for co-prescription pattern discovery. Moreover, implementing level-based ARM was effective for detecting difficult-to-spot rules. Additionally, the majority of drugs prescribed by physicians were consistent with the Standards of Medical Care in Diabetes.
Alteration of the resting-state brain networks: a multi-aspect study of functional and effective connectivity in Parkinson's disease
Authors: Ahmadimehr Shakiba
Published: November 2022

Purpose
Parkinson disease (PD) is known as the second most common and growing neurodegenerative disorder in the world. It is widely known that the appearance of motor and non-motor symptoms causes disorders in the brain network of such patients.
Methods
This study evaluates the brain connectivity of PD patients' (n = 15) in comparison with healthy individuals (n = 15) using functional magnetic resonance imaging (fMRI). First, independent component analysis (ICA) was implemented on the preprocessed data to extract resting-state networks (RSNs) as functional connectivity (FC) for evaluating intra-network connectivity values. Granger causality analysis (GCA) and transfer entropy (TE) are extracted as effective connectivity (EC) methods for assessing the network and regional time courses for assessing inter and intra-network connectivity measures. Finally, F-test was used to find the discriminative regions between the groups.
Results
Thirty ICA maps were identified as independent components, and seven were selected as RSNs. In terms of spatial maps, we found that patients had altered connectivity between Auditory and dorsal Default Mode Network. Several networks and regions were also discriminative between the groups in inter and intra network connectivity analysis, respectively.
Conclusions
The results show that EC methods such as GCA and TE are promising in extracting local information of PD. The reason can be considered in terms of being directional and causal in this type of connectivity, which is in relation to the concept of neuronal substrates. Also, TE might be more accurate than GCA, since TE is nonlinear which is consistent with the nature of the data. To the best of our knowledge, there was not any research that employed EC and ICA on Parkinson's resting-state fMRI data, and analyzed it using ICA time courses as well as regional time series.
An isomorphic valency transition in SmTe film enabling nonvolatile resistive change
Authors: Hatayama Shogo , Mori Shunsuke , Saito Yuta , Fons Paul , Shuang Yi , Sutou Yuji
Published: November 2022

A NaCl-type SmTe film showed remarkable electrical contrast (> 105) between as-deposited and annealed films in the absence of a transition in the crystal structure. A rigid-band shift induced by a valency transition between Sm2+ and Sm3+ was found to give rise to significant change in the electronic structure along with a concomitant resistivity change. Moreover, operation of a SmTe-based memory device was demonstrated by a standard measurement scheme with switching driven by electrical pulse-induced Joule heating. Since SmTe remains crystalline in both resistive states, resistance drift is suppressed offering improved readout reliability. Furthermore, the SmTe films were found to exhibit much better data retention, maintaining data for 10 years at 262 °C in contrast to conventional nonvolatile recording material such as Ge-Sb-Te (85-110 °C). The use of a valency transition reveals a new approach to the development of nonvolatile high performance resistive change materials.
Effect of post-milling process on the oxidation on the rice bran
Authors: Najamuddin Ulfah , Gorji Sara Ghorbani , Fitzgerald Melissa
Published: November 2022

The rancidity of rice bran is predominantly triggered by lipophilic enzyme activities. Bran stabilisation and defatting are the typical processes to inactivate the processes that cause rancidity. However, little is known about how this impacts the development of volatile compounds related to odour in the rice bran. The effects of post-milling treatments, including the stabilisation and defatting process, on the nutritional properties and then on the development of secondary volatile oxidation compounds in the rice bran during storage were investigated. Butanal, hexanal, 2 hexenal, butanoic acid, hexanoic acid, pentanoic acid, and heptanoic acid were the discriminant volatile compounds in the non-defatted bran that were identified as the markers of rancidity of non-defatted stabilised rice. It can thus be suggested that the defatting process positively contributed to the low abundance of volatile oxidation products that are responsible for bran rancidity by slowing down the formation of lipid-derived oxidation products.
Platelet to lymphocyte ratio is a risk factor for failure of non-operative treatment of colonic diverticulitis
Authors: Kim Jong Ho , Han Sang Hyup , Lee Jin-Won , Kim Haesung , Han Jeonghee
Published: November 2022

Introduction:
Non-operative treatment is the mainstay of colonic diverticulitis, but some patients require surgery due to non-operative treatment failure. This study aims to identify risk factors for the failure of non-operative treatment of colonic diverticulitis.
Methods:
From January 2011 to December 2020, we retrospectively reviewed 2362 patients with non-operative treatment for first-attack acute diverticulitis. Patients were categorized into non-operative treatment success or failure groups. Clinical characteristics and serum inflammatory markers were analyzed by multivariable logistic regression to determine risk factors for non-operative treatment failure of colonic diverticulitis.
Results:
Overall, 2.2% (n = 50) of patients underwent delayed surgery within 30 days (median 4.0 [ 3.0; 8.0]) due to non-operative treatment failure. Multivariable logistic regression identified that platelet to lymphocyte ratio (odds ratio [OR], 4.2; 95% confidence interval [CI], 0.05–0.13; p < 0.001), diabetes mellitus (OR, 2.2; 95% CI, 0.01–0.09; p = 0.025), left-sided colonic diverticulitis (OR, 4.1; 95% CI, 0.04–0.13; p < 0.001), and modified Hinchey classification (OR, 6.2; 95% CI, 0.09–0.17; p< 0.001) were risk factors for non-operative treatment failure.
Conclusions:
Platelet to lymphocyte ratio (PLR) is a potential risk factor for the non-operative treatment failure of acute first-attack colonic diverticulitis. Therefore, patients with higher PLR during non-operative treatment should be monitored with special caution.
Deep sea cold seep is an atmospheric Hg sink and MeHg source
Authors: Li Jiwei , Dong Xiyang , Tang Yongjie , Zhang Chuwen , Yang Yali , Zhang Wei , Liu Shanshan , Feng Xinbin , Shang Lihai , Peng Xiaotong
Published: November 2022

Mercury (Hg) is an important element in seafloor cold seeps that might govern methane emission. However, so far, the knowledge of biogeochemical Hg cycle in it remains poorly understood. In this study, Hg geochemical characteristics and microorganisms involved in Hg biogeochemical cycling were examined in three (active, inactive seep vs reference) different types of sediments sampled from the Haima cold seep in the South China Sea. Sediments in the active seep area were significantly enriched in mercury and methylmercury (MeHg) compared to the reference. Accordingly, abundant genes related to Hg methylation (hgcAB), demethylation (merB) and reduction (merA) were detected in the active seep sediments, phylogenetically associated with various bacterial and archaeal linages (e.g.Desulfobacterota, GammaproteobacteriaandHalobacteriota). Hg odd-mass number isotopes (Δ199Hg and Δ201Hg) pointed to their source from the upper ocean and the occurrence of abiotic dark oxidation. The δ202Hg values indicate Hg mass fractionation, migration and transformation in the active seep sediments. These geochemical and microbial data highlight active Hg biogeochemical cycles in seafloor cold seeps, functioning as important Hg-sinks and MeHg sources in the deep ocean.