bluuebunny/crossref_metadata_2025 huggingface dataset:
The Magic Realist Unconscious: Twain, Yamashita and Jackson
Authors: Tatsumi Takayuki
Published: October 2022

The literary topic of Siamese twins is not unfamiliar. American literary history tells us of the genealogy from Mark Twain’s pseudo-antebellum story The Tragedy of Pudd’nhead Wilson and the Comedy Those Extraordinary Twins (1894), Karen Tei Yamashita’s postmodern metafiction “Siamese Twins and Mongoloids: Cultural Appropriation and the Deconstruction of Stereotype via the Absurdity of Metaphor” (1999), down to Shelley Jackson’s James Tiptree, Jr. award winner Half-Life (2006). Rereading these works, we are easily invited to notice the political unconscious hidden deep within each plot: Twain’s selection of the Italian Siamese twins based upon Chang and Eng Bunker, antebellum stars of the Barnum Museum, cannot help but recall the ideal of the post-Civil War world uniting the North and the South; Yamashita’s figure of the conjoined twins Heco and Okada derives from Hikozo Hamada, an antebellum Japanese who made every effort to empower the bond between Japan and the United States, and John Okada, the Japanese American writer well known for his masterpiece No No Boy (1957); and Jackson’s characterization of the female conjoined twins Nora and Blanche Olney represents a new civil rights movement in the post-Cold War age in the near future, establishing a close friendship between the humans and the post-humans. This literary and cultural context should convince us that Yamashita’s short story “Siamese Twins and Mongoloids” serves as a kind of singularity point between realist twins and magic realist twins. Influenced by Twain’s twins, Yamashita paves the way for the re-figuration of the conjoined twins not only as tragi-comical freaks in the Gilded Age but also as representative men of magic realist America in our Multiculturalist Age. A Close reading of this metafiction composed in a way reminiscent of Jorge Luis Borges, Stanislaw Lem and Bruce Sterling will enable us to rediscover not only the role conjoined twins played in cultural history, but also the reason why Yamashita had to feature them once again in her novel I Hotel (2010) whose plot centers around the Asian American civil rights movement between the 1960s and the 1970s. Accordingly, an Asian American magic realist perspective will clarify the way Yamashita positioned the figure of Siamese Twins as representing legal and political double standards, and the way the catachresis of Siamese Twins came to be naturalized, questioned and dismissed in American literary history from the 19th century through the 21st century.
Oil Change Interval Evaluation of Gearbox Used in Heavy-Duty Truck E-Axle with Oil Analysis Data
Authors: Yan Shufa , Kong Zhuo , Liu Hongwei , Li Bin , Fan Mao , Zhang Xiaokun
Published: October 2022

Regular lubricating oil change in the gearbox is desirable for improving vehicle reliability and reducing operating costs. To achieve this objective, evaluating the oil change interval is necessary. However, due to the complex and dynamic properties of oil degradation, oil change interval evaluation has been a bottleneck in practice. Therefore, a solution strategy is proposed in this paper that utilizes the oil physicochemical properties derived from oil analysis data to determine the optimal oil change interval. With a large amount of oil analysis data collected, the iron (Fe) debris, kinematic viscosity (100 °C), and total acid number (TAN) are considered to be the oil change indicators of lubricating oil. By monitoring the changes in the selected oil change indicators, linear regression is firstly applied to the original oil analysis data to reveal the dynamic degradation process. Then, the Wiener-based stochastic process is used to describe the first hitting time and the increasing trends of the selected oil change indicator. Finally, the oil change interval can be obtained under the concept of the first hitting time. Compared with the planned maintenance time, the proposed method seems reasonable considering the dynamic property of oil degradation. The effectiveness of the proposed method is evaluated using a case study with an oil analysis dataset from an E-axle with a two-shift gearbox. The results show that the oil change interval increased by approximately 10,000 km (50%) compared with the planned maintenance interval. This will reduce vehicle maintenance time and save maintenance costs.
Viability Criteria during Liver Ex-Situ Normothermic and Hypothermic Perfusion
Authors: Melandro Fabio , De Carlis Riccardo , Torri Francesco , Lauterio Andrea , De Simone Paolo , De Carlis Luciano , Ghinolfi Davide
Published: October 2022

With the increased use of extended-criteria donors, machine perfusion became a beneficial alternative to cold storage in preservation strategy for donor livers with the intent to expand donor pool. Both normothermic and hypothermic approach achieved good results in terms of mid- and long-term outcome in liver transplantation. Many markers and molecules have been proposed for the assessment of liver, but no definitive criteria for graft viability have been validated in large clinical trials and key parameters during perfusion still require optimization.In this review, we address the current literature of viability criteria during normothermic and hypothermic machine perfusion and discuss about future steps and evolution of these technologies.
Pulmonary Involvement in SARS-CoV-2 Infection Estimates Myocardial Injury Risk
Authors: Dumea Eduard , Lazar Mihai , Barbu Ecaterina Constanta , Chitu Cristina Emilia , Ion Daniela Adriana
Published: October 2022

Background and Objectives: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection represents a pathology with primary pulmonary involvement and multisystemic impact, including cardiovascular injuries. The present study aimed to assess the value of clinical, biochemical, and imaging factors in COVID-19 patients in determining the severity of myocardial involvement, and to create a model that can be used toevaluate myocardial injury risk based on clinical, biochemical and imaging factors. Materials and Methods: We performed an observational cohort study on 150 consecutive patients, evaluating their age, sex, hospitalization period, peripheral oxygen saturation (SpO2) in ambient air, systolic and diastolic blood pressure, heart rate, respiratory rate, biochemical markers of cardiac dysfunction (TnI, and NT-proBNP), inflammatory markers (C reactive protein (CRP), fibrinogen, serum ferritin, interleukin-6 (IL-6), tumor necrosis factor alpha (TNFα)), D-dimers, lactate dehydrogenase (LDH), myoglobin and radio-imaging parameters. All patients underwent computerized tomography chest scan in the first two days following admission. Results: We observed elevated heart and respiratory rates, higher systolic blood pressure, and a lower diastolic blood pressure in the patients with cardiac injury; significant differences between groups were registered in TnI, NT-proBNP, LDH, CRP, and D-dimers. For the radiological parameters, we found proportional correlations with the myocardial injury for the severity of lung disease, number of pulmonary segments with alveolar consolidation, number of pulmonary lobes with pneumonia, crazy paving pattern, type of lung involvement, the extent of fibroatelectatic lesions and the mediastinal adenopathies. Conclusions: Myocardial injury occurred in 12% of patients in the study group. Ground glass opacities, interstitial interlobular septal thickening (crazy paving pattern), fibroatelectasic lesions and alveolar consolidations on CT scan were correlated with myocardial injury. Routine lung sectional imaging along with non-specific biomarkers (LDH, D-dimers, and CRP) can be further valuable in the characterization of the disease burden, thus impacting patient care.
Optimization of Castor Oil-Based Ion Selective Electrode (ISE) with Active Agent 1,10-Phenanthroline for Aqueous Pb2+ Analysis
Authors: Nisah Khairun , Rahmi Rahmi , Ramli Muliadi , Idroes Rinaldi , Alva Sagir , Iqhrammullah Muhammad , Safitri Eka
Published: October 2022

This research has successfully fabricated ion selective electrode (ISE) for Pb2+ using castor oil (Ricinus communis L.)-based polyurethane (PU) membrane with 1,10-phenanthroline as the active agent. The sensitivity of the Pb2+ ISE obtained is 27.25 mV/decade with a linear range of [Pb(NO3)2] of 10−10–10−5 M and a coefficient of determination (R2) of 0.959. The system response reaches stability after 25 s of measurement. The Pb2+ has a detection limit of 10−10 M and gives a stable response at pH 7–8 with a 15-day lifetime. The investigation of the selectivity of the ISE was performed using the mixed solution method with log Kij values of <1. The selectivity order of Pb2+ ISE against the foreign ions is Ag2+ > Ca2+ > K+ > Mg2+ > Cu2+ > Fe3+ > Cr3+> Zn2+ > Cd2+. The Pb2+ ISE shows acceptable reproducibility and repeatability with standard deviation values of 0.065 and 0.0079, respectively. Fourier transform infrared (FT-IR) spectra confirmed that 1,10-phenanthroline was responsible for the formation of the Pb2+ ion entrapment via complexation. Other characterizations (crystallinity, micro-surface morphology, and mechanical strength) suggest the degradation of the membrane structure integrity after the application. The analysis results of Pb levels using the Pb2+ ISE in artificial and wastewater samples were not significantly different from the atomic absorption spectroscopy (AAS) measurement.
Recovery of Valuable Aromas from Sardine Cooking Wastewaters by Pervaporation with Fractionated Condensation: Matrix Effect and Model Validation
Authors: Pereira M. João , Pintado Manuela , Brazinha Carla , Crespo João
Published: October 2022

Due to the lack of studies addressing the influence of real food matrices on integrated organophilic pervaporation/fractionated condensation processes, the present work analyses the impact of the real matrix of sardine cooking wastewaters on the fractionation of aromas. In a previous study, a thermodynamic/material balance model was developed to describe the integrated pervaporation—a fractionated condensation process of aroma recovery from model solutions that emulate seafood industry aqueous effluents, aiming to define the best conditions for off-flavour removal. This work assesses whether the previously developed mathematical model, validated only with model solutions, is also applicable in predicting the fractionation of aromas of different chemical families from real effluents (sardine cooking wastewaters), aiming for off-flavour removals. It was found that the food matrix does not influence substantial detrimental consequences on the model simulations, which validates and extends the applicability of the model.
Modelling of Single-Gas Adsorption Isotherms
Authors: Chilev Chavdar , Dicko Moussa , Langlois Patrick , Lamari Farida
Published: October 2022

The present paper reviews and analyses different models that could be used to describe the adsorption equilibrium of pure gases. The adsorption equilibrium of hydrogen, nitrogen and methane is characterised and modelled. Several thermodynamic conceptions have been selected and tested for the calculation of physico-chemical parameters. The model of Dubinin has been selected to estimate the pseudo-saturation vapour pressure. The best results for the molar volume of the adsorbate have been obtained by using Do’s equation. Eight models, namely Langmuir, Freundlich, Sips, Toth, Jovanovic, UNILAN, OBMR and Potential Theory, describing the adsorption equilibrium of pure gases, have been tested and compared with experimental data obtained from the literature at three different temperatures (283 K, 298 K and 313 K). In order to determine the best fit, the correlation coefficient and the standard errors for each parameter have been used to evaluate the data. All the models used in this study, except for Freundlich’s equation in the case of nitrogen or methane adsorption, are in good agreement between experiment and modelling for the adsorption isotherms.
Effect of Roll Material on Strip Solidification between the Rolls of a Vertical-Type High-Speed Twin-Roll Caster
Authors: Haga Toshio , Kurahashi Yukihiro
Published: October 2022

Tool steel for hot working is usually used for the rolls of conventional twin-roll casters for aluminum alloys. In a vertical high-speed twin-roll caster, copper rolls are used to rapidly solidify the aluminum alloys and to increase the casting speed, since the thermal conductivity of copper is higher than that of tool steel. However, the degree of superiority of copper rolls over steel rolls is not clear. In the present study, the effect of the roll material on strip solidification between the rolls of a vertical high-speed twin-roll caster was investigated by inserting a thermocouple in the strip during casting. The Al-4.8%Mg alloy was used, since it does not stick to the steel rolls in the absence of a parting material. Both copper rolls and mild steel rolls were used. The cooling rate, the supercooling of the liquidus temperature, and the temperature of the strip cast using the copper rolls were superior to those of that cast using the mild steel rolls. The copper rolls were better than the steel rolls in terms of achieving high-speed roll casting, rapid solidification of the strip, and high productivity.
Fatigue Performance Evaluation of K-Type Joints in Long-Span Steel Truss Arch Bridge
Authors: Liu Peng , Lu Hongping , Chen Yixuan , Zhao Jian , An Luming , Wang Yuanqing
Published: October 2022

The K-type joint, which consists of the web members and the chord members with varied angles welded together, has been widely adopted in long-span steel truss bridges. However, its fatigue performance has been rarely considered, despite its critical role in bridge structural safety and durability. Accordingly, the FE model of the K-type joint was established in Abaqus and the fatigue performance analysis was conducted, in which the effect of web/chord thickness ratio (τ), chord/web angle (θ), and chord with rib stiffener were investigated. Take the Mingzhu Bay steel truss arch bridge as an engineering background, the hot spot stress method was employed to calculate the fatigue performance of three K-type joints in unfavorable locations. Furthermore, a 3D full-scall bridge model was built to evaluate the fatigue performance of the K-type joints under standard and overloaded moving vehicle load scenarios. The results show that the max hot spot stress factor (SCFmax) of the web and chord member is influenced by τ and θ. The chord members added stiffener is founded to be an effective way to enhance fatigue performance. The fatigue stress intensities of the three unfavorable locations meet the Eurocode 3 specification requirements, but the one in the mid-truss arch is not satisfied under an overloaded vehicle loading rate of 25%.
Effect of Structure of Carbonized Polymer Dot on the Mechanical and Electrical Properties of Copper Matrix Composites
Authors: Xu Jiahui , Zhao Wenmin , Li Shaoyu , Bao Rui , Yi Jianhong , Li Zhaojie
Published: October 2022

Carbonized polymer dots (CPDs) have been paid a lot of attention by researchers because of their excellent properties due to their unique structure. However, few studies have researched the impact of the CPD structure on composite applications. Herein, CPD and heat-treated CPD (h-CPD) are used to fabricate a reinforced Cu matrix composite. There was a semi-coherent interface between h-CPD and Cu. However, functional groups and polymer chains of CPDs decomposed during heat treatment, weakening the mechanical and electrical properties of the composites. These findings show that CPD structural integrity is vital to composites.
Corrosion and Electrochemical Properties of Laser-Shock-Peening-Treated Stainless Steel AISI 304L in VVER Primary Water Environment
Authors: Arnoult Xavier , Arnoult-Růžičková Mariana , Maňák Jan , Viani Alberto , Brajer Jan , Arrigoni Michel , Kolman Radek , Macák Jan
Published: October 2022

Laser Shock Peening (LSP) is a surface treatment technique for metallic materials. It induces plastic deformation at the surface of up to around 1 mm in depth. This process introduces residual stresses that lead to strain hardening, and potentially improvements in fatigue, stress corrosion cracking (SCC) and general corrosion behaviour in many, but not all, corrosive media. In this paper, two specimens made of AISI 304L stainless steel, one LSP-treated and one un-treated, were tested at 280 °C and 8 MPa in VVER (or PWR) primary circuit water chemistry using in situ Electrochemical Impedance Spectroscopy (EIS). This experiment serves to qualify the influence of LSP on the changes in corrosion behaviour in high-temperature, high-density water. The residual stress (RS) measurement of the surface showed a compression RS. Before LSP treatment, RS at the surface was 52.2 MPa in the rolling direction 0°RD and 10.42 MPa in the transverse rolling direction 90°RD. After the treatment, surface RS was −175.27 MPa and −183.51 MPa for Scan and TScan directions, respectively. The effect of compressive RS at the surface was studied and showed an increase in corrosion rate. The analysis of oxide layer by SEM revealed differences between LSP-treated and untreated AISI 304L specimens and their connection to corrosion rates.
Hydraulic Jump Behavior during Filling Process of Cast Start II
Authors: Chen Jin , Zhang Lei , Cui Pan , Zhang Jiageng , Luan Guowei , Wang Fei
Published: October 2022

This paper presents a 0.25-scale quasi-three-dimensional hydraulic model experiment of transient fluid flow phenomena during continuous casting of steel under solidification. Water cooling is performed at the bottom of the mold, and saturated sodium acetate solution is covered on the spring and particle arrangement at the bottom of the mold/on the dummy bar to simulate the solidification phenomenon in the actual continuous casting process. The results show that the solidification on the dummy bar surface is very important to the early stage of the filling process and cannot be ignored. Its influence gradually decreases with the rise of the liquid level, and basically disappears when the liquid level rises to a certain height (steady liquid level, SLL). In SLL, the interface morphology of the solidified layer is more affected by the flow, while the interface morphology of the solidified layer has no significant effect on the liquid surface velocity.
Simplifying the B Complex: How Vitamins B6 and B9 Modulate One Carbon Metabolism in Cancer and Beyond
Authors: Franco Carolina N. , Seabrook Laurence J. , Nguyen Steven T. , Leonard Jack T. , Albrecht Lauren V.
Published: October 2022

Vitamin B micronutrients are essential regulators of one carbon metabolism that ensures human health. Vitamin B9, or folate, lies at the heart of the folate cycle and converges with the methionine cycle to complete the one carbon pathway. Additionally, vitamin B6 contributes by orchestrating the flux of one carbon cycling. Dysregulation of vitamin B contributes to altered biochemical signaling that manifests in a spectrum of human diseases. This review presents an analysis of the past, present, and future work, highlighting the interplay between folate and vitamin B6 in one carbon metabolism. Emerging insights include advances in metabolomic-based mass spectrometry and the use of live-cell metabolic labeling. Cancer is used as a focal point to dissect vitamin crosstalk and highlight new insights into the roles of folate and vitamin B6 in metabolic control. This collection of vitamin-based research detailing the trends of one carbon metabolism in human disease exemplifies how the future of personalized medicine could unfold using this new base of knowledge and ultimately provide next-generation therapeutics.
A Novel Approach for Measurement of Free-Form Optical Elements with Digital Holographic Microscopy
Authors: Zhang Xuhui , Wu Chonglin , Chen Lei , Chen Gengliang , Zheng Guoliang
Published: October 2022

Free-form optical elements face significant challenges in high-precision measurement due to their high complexity and non-rotational symmetry. Digital holographic microscopy (DHM), as one of the methods for the measurement of free-form optical elements, has promising applications due to its ultra-high precision and non-destructive and fast characteristics. Therefore, we have designed a novel measurement method that combines transmission DHM and reflection DHM to obtain thickness information and surface information of elements to deduce the 3D structure. With this method, we completed the measurement of a free-form optical element. The DHM system we built has recorded holograms under 4× and 20× objectives and successfully recovered the 3D surface shape of the element. The measurements are consistent with the designed and manufactured parameters, demonstrating the unique advantages of DHM for measuring special types of optical elements.
Comprehensive Characterization Integrating Static and Dynamic Data for Dynamic Fractures in Ultra-Low Permeability Reservoirs: A Case Study of the Chang 6 Reservoir of the Triassic Yanchang Formation in the Ordos Basin, China
Authors: Wang Youjing , Song Xinmin
Published: October 2022

The generation of dynamic fractures during the process of water injection in ultra-low permeability reservoirs aggravates the heterogeneity of the reservoir, resulting in a rapid rise of water cut and directional flooding of the producers, which affects waterflood sweep efficiency and recovery. A dynamic fracture, as the geological feature of ultra-low permeability reservoirs, has a complex genetic mechanism and is difficult to characterize. Taking the Chang 6 reservoir of the Triassic Yanchang Formation in the Ordos Basin of central China as an example, this paper presents the characterization method and workflow of dynamic fractures. On the one hand, through the analysis of triaxial-compression rock-mechanic experiments and the mineral composition of the core, we evaluated rock brittleness in order to identify the lithology that can easily generate new fractures. On the other hand, beginning with the ancient tectonic stress field and combining the fracture characteristics of core and geological outcrop, the multi-fractal method and the probabilistic neural network were applied to identify the natural fractures and to quantitatively predict the intensity of natural fractures. Based on the rock brittleness evaluation and the natural fracture feature, the intensity of dynamic fractures was characterized by integrating the analysis of the bottom hole pressure, fracture pressure, and production response characteristics. A dynamic fracture is a “double-edged sword” during the waterflooding development of ultra-low permeability reservoirs. The premature activation and generation of dynamic fractures could lead to a worse development status. Nevertheless, the rational control and utilization of dynamic fractures play a positive role in improving oil recovery. Dynamic fractures are of great significance to the optimization and adjustment of well patterns for ultra-low permeability reservoirs. This can provide a reference for similar reservoirs.
Characterization of Discarded Lead–Zinc Sulfide Ore Tailings Based on Mineral Fragments
Authors: Zhao Jianqi , Hu Wentao , Xiao Fusheng , Liu Xinwei , Yu Hongdong , Yuan Huan , Wang Huajun
Published: October 2022

The lead–zinc ore is a typical low-grade complex polymetallic sulphide ore. Its typical mineralogy is characterized by a high content of cryptocrystalline graphite, low content of lead, zinc, and copper, and fine grain size. However, the aggregation size of sulfide mineral aggregations is coarse, and the symbiotic relationship is relatively simple. Conventional process treatment requires the fine grinding of ore into mineral monomer dissociations followed by preferential flotation, but this method has a poor sorting index and high production cost. In this paper, the grain size of the sulfide mineral aggregates determined by mineralogical studies is used to determine the fineness of the grinding, so that the liberation degree of the sulfide ore in the coarse grinding product reaches 70%, and each flotation process is used to collect rough concentrate. In the first step of flotation, the carbon in lead–zinc sulfide ore was removed by adding #2 oil to the flotation tank at a dosage of 40 g/t. In the second flotation process, the pH was adjusted to 7.2, the dosage of isopropyl xanthate was 20 g/t, the dosage of #2 oil was 30 g/t; the flotation reagent in the third step was isopropyl xanthate, and the dosage was 7.5 g/t, with a #2 oil of 15 g/t. Each flotation process concentrate is collected. Finally, the grades of lead and zinc in the final concentrate were 1.6% and 5.71%, respectively. In addition, the recoveries of lead and zinc were 91.78% and 92.07%, respectively. The yield of tailings was 50.6%. By the flotation of sulfide aggregates, a large number of gangues are avoided to participate in fine grinding, which helps to reduce the energy consumption of the mill.
Development of Glycyrrhetinic Acid and Folate Modified Cantharidin Loaded Solid Lipid Nanoparticles for Targeting Hepatocellular Carcinoma
Authors: Xu Yilin , Wang Min , Ning Shuangcheng , Yang Zhonglan , Zhou Lili , Xia Xinhua
Published: October 2022

Cantharidin (CTD) is the major component of anticancer drugs obtained from Mylabris Cichorii and has a good inhibitory effect on several cancers, including hepatocellular carcinoma (HCC) and breast cancer. However, due to its toxicity, oral administration can cause various adverse reactions, limiting its clinical application. The aim of this work was to design glycyrrhetinic acid (GA)- and/or folate (FA)-modified solid lipid nanoparticles (SLNs) for the encapsulation of CTD to target HCC. Four CTD-loaded SLNs (cantharidin solid lipid nanoparticles (CSLNs), glycyrrhetinic acid-modified cantharidin solid lipid nanoparticles (GA-CSLNs), folate-modified cantharidin solid lipid nanoparticles (FA-CSLNs), and glycyrrhetinic acid and folate-modified cantharidin solid lipid nanoparticles (GA-FA-CSLNs)) were prepared by the emulsion ultrasonic dispersion method, and their physicochemical parameters were determined (particle size and distribution, morphology, zeta-potential, entrapment efficiency, drug loading, and hemolysis). Additionally, the antitumor activities of the four SLNs were evaluated comprehensively by tests for cytotoxicity, cell migration, cell cycle, apoptosis, cellular uptake, competition suppression assay, and in vivo tumor suppression assay. Four SLNs showed spherical shapes and mean diameters in the range of 75–110 nm with size dispersion (PDI) within the range of 0.19–0.50 and zeta-potential approximately –10 mV. The entrapment efficiency of CTD in SLNs was higher than 95% for all tested formulations, and no hemolysis was observed. Compared to GA-CSLNs or CSLNs, GA-FA-CSLNs and FA-CSLNs showed stronger cytotoxicity on hepatocellular carcinoma cells (HepG2), and the cytotoxicity of GA-FA-CSLNs on hepatocyte cells (L-02) was remarkably reduced compared with other formulations. GA-FA-CSLNs and FA-CSLNs also increased the inhibition of HepG2 cell migration, and FA-CSLNs had the highest apoptosis rate. The cell cycle results indicated that HepG2 cells were arrested mainly in the S phase and G2/M phase. Analysis of competition inhibition experiments showed that GA and FA ligands had targeted effects on HepG2 cells. The in vivo tumor inhibition experiment showed that GA-FA-CSLNs and FA-CSLNs had excellent tumor inhibition ability—their tumor inhibition rates were 96.46% and 89.92%, respectively. Our results indicate that GA-FA-CSLNs and FA-CSLNs have a promising future in the therapeutic intervention of HCC.
Alzheimer’s Disease as a Major Public Health Concern: Role of Dietary Saponins in Mitigating Neurodegenerative Disorders and Their Underlying Mechanisms
Authors: Abduljawad Asaad A. , Elawad Mohammed Ahmed , Elkhalifa Modawy Elnour Modawy , Ahmed Alshebli , Hamdoon Alashary Adam Eisa , Salim Liga Hasan Mohammed , Ashraf Muhammad , Ayaz Muhammad , Hassan Syed Shams ul , Bungau Simona
Published: October 2022

Saponins are triterpenoid or steroidal glycosides and are an important group of naturally occurring compounds of plant origin. They exhibit diverse pharmacological potentials including radical scavenging, as well as neuroprotective, anti-diabetic and anti-inflammatory activities, owing to their diverse chemical scaffolds. Saponins consist of an aglycone part (non-sugar) and a glycone part (sugar) and have at least one glycosidic (C–O sugar bond) linkage present between the glycone and aglycone mostly at C-3. On the basis of the aglycone part, saponins are classified into triterpenoid glycosides, steroid glycosides and alkaloid glycosides. Saponins exhibit neuroprotective activities against various disorders of the central nervous system (CNS) including stroke, Alzheimer’s disease (AD), Huntington’s disease (HD) and Parkinson’s disease (PD). They mediate their therapeutic effects by modulation of various pathological targets. This study highlights various neuroprotective mechanisms of saponins including free radical scavenging, modulation of neuroprotective signaling pathways, activation of neurotrophic factors, modulation of neurotransmitters, inhibition of BACE1 enzyme and tau hyper-phosphorylation. The study concludes that saponins have considerable efficacy against various pathological targets of neurological disorders, especially AD, and might be an important source of leads against neurodegenerative disorders.
Hesperetin—Between the Ability to Diminish Mono- and Polymicrobial Biofilms and Toxicity
Authors: Carević Tamara , Kostić Marina , Nikolić Biljana , Stojković Dejan , Soković Marina , Ivanov Marija
Published: October 2022

Hesperetin is the aglycone of citrus flavonoid hesperidin. Due to the limited information regarding hesperetin antimicrobial potential and emerging need for novel antimicrobials, we have studied its antimicrobial activity (microdilution assay), antibiofilm activity with different assays in two models (mono- and polymicrobial biofilm), and toxicity (MTT and brine shrimp lethality assays). Hesperetin inhibited growth of all Candida isolates (minimal inhibitory concentration, MIC, 0.165 mg/mL), while it’s inhibitory potential towards Staphylococcus aureus was lower (MIC 4 mg/mL). Hesperetin (0.165 mg/mL) reduced ability of Candida to form biofilms and moderately reduced exopolysaccharide levels in biofilm matrix. Effect on the eradication of 24 h old C. albicans biofilms was promising at 1.320 mg/mL. Inhibition of staphylococcal biofilm formation required higher concentrations of hesperetin (<50% inhibition with MIC 4 mg/mL). Establishment of polymicrobial C. albicans-S. aureus biofilm was significantly inhibited with the lowest examined hesperetin concentration (1 mg/mL) in crystal violet and CFU assays. Hesperetin toxicity was examined towards MRC-5 fibroblasts (IC50 0.340 mg/mL) and in brine shrimp lethality assay (LC50 > 1 mg/mL). Hesperetin is efficient in combating growth and biofilm formation of Candida species. However, its antibacterial application should be further examined due to the cytotoxic effects provoked in the antibacterial concentrations.
Preface to Nitric Oxide Modulators in Health and Disease I
Authors: Maccallini Cristina , Amoroso Rosa
Published: October 2022

Nitric oxide (NO) is a small free radical molecule biosynthesized by nitric oxide synthases (NOS), a family of oxidoreductases responsible for the conversion of the natural substrate L-arginine into L-citrulline and NO [...]
Rigorous Analysis and Systematical Design of Double-Layer Metal Superlens for Improved Subwavelength Imaging Mediated by Surface Plasmon Polaritons
Authors: Wang Jing , Li Zhichao , Liu Weina
Published: October 2022

A double-layer metal superlens was rigorously analyzed and systematically designed to improve subwavelength imaging ability. It was revealed that transmission properties of the imaging system could be accurately interpreted by the five-layer waveguide mode theory—each amplification peak among the spatial frequency range of evanescent waves was associated with a corresponding surface plasmon polariton (SPP) mode of an insulator-metal-insulator-metal-insulator (IMIMI) structure. On the basis of such physical insight, evanescent waves of higher spatial frequency were effectively amplified via increasing propagation constants of symmetrically coupled short-range SPP (s-SRSPP) and antisymmetrically coupled short-range SPP (a-SRSPP), and evanescent waves of lower spatial frequency were appropriately diminished by approaching to cut off symmetrically coupled long-range SPP (s-LRSPP). A flat and broad optical transfer function of the imaging system was then achieved, and improved subwavelength imaging performance was validated by imaging an ideal thin object of two slits with a 20-nm width distanced by a 20-nm spacer, under 193-nm illumination. The resolution limit of the designed imaging system with double-layer superlens was further demonstrated to be at least ~λ/16 for an isolated two-slit object model. This work provided sound theoretical analysis and a systematic design approach of double-layer metal superlens for near-field subwavelength imaging, such as fluorescent micro/nanoscopy or plasmonic nanolithography.
Enhanced Electrochemical Performance of Sugarcane Bagasse-Derived Activated Carbon via a High-Energy Ball Milling Treatment
Authors: Wannasen Likkhasit , Chanlek Narong , Siriroj Sumeth , Maensiri Santi , Swatsitang Ekaphan , Pinitsoontorn Supree
Published: October 2022

Activated carbon (AC) from sugarcane bagasse was prepared using dry chemical activation with KOH. It was then subjected to a high-energy ball milling (HEBM) treatment under various milling speeds (600, 1200 and 1800 rpm) to produce AC nanoparticles from micro-size particles. The AC samples after the HEBM treatment exhibited reduced particle sizes, increased mesopore volume and a rich surface oxygen content, which contribute to higher pseudocapacitance. Notably, different HEBM speeds were used to find a good electrochemical performance. As a result, the AC/BM12 material, subjected to HEBM at 1200 rpm for 30 min, exhibited the highest specific capacitance, 257 F g−1, at a current density 0.5 A g−1. This is about 2.4 times higher than that of the AC sample. Moreover, the excellence capacitance retention of this sample was 93.5% after a 3000-cycle test at a current density of 5 A g−1. Remarkably, a coin cell electrode assembly was fabricated using the AC/BM12 material in a 1 M LiPF6 electrolyte. It exhibited a specific capacitance of 110 F g−1 with a high energy density of 27.9 W h kg−1.
Elucidation of Strain-Dependent, Zinc Oxide Nanorod Response for Nanorod-Guided Fluorescence Intensity
Authors: Truong Johnson , Stoner Andrew , Sytu Marion Ryan C. , Tatlock T Rizana , Cho David H. , Hahm Jong-in
Published: October 2022

In this work, we examine how strain exerted on individual ZnO nanorods (NRs) can influence the fluorescence signals that are emitted from fluorophore molecules and subsequently coupled into and guided along the NR. We elucidate the relationships between the incremental levels of compressive and tensile strain on the NRs and measured fluorescence intensity of a model fluorophore, rhodamine 6G (R6G), as a function of the position on the NRs. We reveal that compressive strain on the NRs leads to a decrease in the guided fluorescence signal, while tensile strain leads to an increase in the fluorescence intensity. Compared to an unstrained state, approximately 35% decrease (increase) in R6G fluorescence intensity was observed from ZnO NRs when they were under compressive strain of −14% (tensile strain of +10%). Further, our systematic acquisition of the incremental addition of uniaxial strain result in a linear relationship of the coupled fluorescence signal and the amount of applied strain. The degree of fluorescence intensification on nanorod ends (DoF), which is a quantitative indicator for the amount of R6G signals coupled into and waveguided to the NR ends compared to those on the main body, also exhibits a linear relationship with strain. These outcomes, in turn, demonstrate that strain alters the waveguiding capabilities of ZnO NRs in a predictable manner, which can be exploited to modulate and optimize fluorescence and other light signals emitted by a nearby source. Considering the wide utility of ZnO NRs in photonics, optoelectronics, and sensors, insights from our study may be highly valuable to effectively controlling and enhancing optical signals from chemical and biological analytes through strain.
Corrugations in Free-Standing Graphene
Authors: Singh Rajendra , Scheinecker Daniel , Ludacka Ursula , Kotakoski Jani
Published: October 2022

Although both the tendency of 2D materials to bend out of plane as well as its effect on materials’ properties are well known, the factors influencing this phenomenon have not been extensively studied. Graphene, the one-atom-thick membrane of carbon atoms, is both arguably the best known 2D material, as well as the most prone to spontaneous corrugations. Here, we use electron diffraction to systematically study the factors influencing corrugations in graphene, including the size of the free-standing area, the preparation method, the amount of surface contamination, and electron-beam-induced structural disorder. We find that mechanically exfoliated graphene is less corrugated than graphene grown via chemical vapor deposition (corrugation amplitude of (0.83±0.10) Å compared to (1.33±0.20) Å for a free-standing area with a diameter of 1.7μm). Similarly, corrugation amplitude grows by more than a factor of two when the diameter of the free- standing area is increased from 1.7μm to ca. 3.0μm. Electron beam irradiation affects the corrugation in two ways, firstly by removing the hydrocarbon contamination, which decreases corrugation, and secondly by creating increasing amounts of disorder into the material, which again increases corrugation. Overall, our results show that control over the sample during both initial preparation and post-preparation treatment allows for a change in the amount of corrugation in free-standing 2D materials, which may lead to new advances in their use in applications.
Spontaneous Imbibition Oil Recovery by Natural Surfactant/Nanofluid: An Experimental and Theoretical Study
Authors: Khoramian Reza , Kharrat Riyaz , Pourafshary Peyman , Golshokooh Saeed , Hashemi Fatemeh
Published: October 2022

Organic surfactants have been utilized with different nanoparticles in enhanced oil recovery (EOR) operations due to the synergic mechanisms of nanofluid stabilization, wettability alteration, and oil-water interfacial tension reduction. However, investment and environmental issues are the main concerns to make the operation more practical. The present study introduces a natural and cost-effective surfactant named Azarboo for modifying the surface traits of silica nanoparticles for more efficient EOR. Surface-modified nanoparticles were synthesized by conjugating negatively charged Azarboo surfactant on positively charged amino-treated silica nanoparticles. The effect of the hybrid application of the natural surfactant and amine-modified silica nanoparticles was investigated by analysis of wettability alteration. Amine-surfactant-functionalized silica nanoparticles were found to be more effective than typical nanoparticles. Amott cell experiments showed maximum imbibition oil recovery after nine days of treatment with amine-surfactant-modified nanoparticles and fifteen days of treatment with amine-modified nanoparticles. This finding confirmed the superior potential of amine-surfactant-modified silica nanoparticles compared to amine-modified silica nanoparticles. Modeling showed that amine surfactant-treated SiO2 could change wettability from strongly oil-wet to almost strongly water-wet. In the case of amine-treated silica nanoparticles, a strongly water-wet condition was not achieved. Oil displacement experiments confirmed the better performance of amine-surfactant-treated SiO2 nanoparticles compared to amine-treated SiO2 by improving oil recovery by 15%. Overall, a synergistic effect between Azarboo surfactant and amine-modified silica nanoparticles led to wettability alteration and higher oil recovery.
Added Sugar, Sugar-Sweetened Beverages, and Artificially Sweetened Beverages and Risk of Cardiovascular Disease: Findings from the Women’s Health Initiative and a Network Meta-Analysis of Prospective Studies
Authors: Yang Bo , Glenn Andrea J. , Liu Qing , Madsen Tracy , Allison Matthew A. , Shikany James M. , Manson JoAnn E. , Chan Kei Hang Katie , Wu Wen-Chih , Li Jie , Liu Simin , Lo Kenneth
Published: October 2022

Much remains unknown about the role of added sugar in relation to cardiovascular disease (CVD) and the relative contributions of sugar-sweetened beverages (SSB) or artificially sweetened beverages (ASB) to CVD risk. Among the 109,034 women who participated in Women’s Health Initiative, we assessed average intakes of added sugar, SSB and ASB, and conducted Cox regression to estimate the hazard ratios (HRs) and their 95% confidence intervals for CVD risk. The consistency of findings was compared to a network meta-analysis of all available cohorts. During an average of 17.4 years of follow-up, 11,597 cases of total CVD (nonfatal myocardial infarction, coronary heart disease (CHD) death, stroke, coronary revascularization, and/or incident heart failure) were confirmed. Added sugar as % energy intake daily (%EAS) at ≥15.0% was positively associated with total CVD (HR = 1.08 [1.01, 1.15]) and CHD (HR = 1.20 [1.09, 1.32]). There was also a higher risk of total CVD associated with ≥1 serving of SSB intake per day (HR = 1.29 [1.17, 1.42]), CHD (1.35 [1.16, 1.57]), and total stroke (1.30 [1.10, 1.53]). Similarly, ASB intake was associated with an increased risk of CVD (1.14 [1.03, 1.26]) and stroke (1.24 [1.04, 1.48]). According to the network meta-analysis, there was a large amount of heterogeneity across studies, showing no consistent pattern implicating added sugar, ASB, or SSB in CVD outcomes. A diet containing %EAS ≥15.0% and consuming ≥1 serving of SSB or ASB may be associated with a higher CVD incidence. The relative contribution of added sugar, SSB, and ASB to CVD risk warrants further investigation.
Food Outlet Access and the Healthiness of Food Available ‘On-Demand’ via Meal Delivery Apps in New Zealand
Authors: Norriss Dru , Crossin Rose , Curl Angela , Bidwell Susan , Clark Elinor , Pocock Tessa , Gage Ryan , McKerchar Christina
Published: October 2022

Access to unhealthy commodities is a key factor determining consumption, and therefore influences the prevalence of non-communicable diseases. Recently, there has been an increase in the availability of food ‘on-demand’ via meal delivery apps (MDAs). However, the public health and equity impacts of this shift are not yet well understood. This study focused on three MDAs in New Zealand and aimed to answer (1) what is the health profile of the foods being offered on-demand, (2) how many food outlets are available and does this differ by physical access or neighbourhood demographics and (3) does the health profile of foods offered differ by physical access or neighbourhood demographics? A dataset was created by sampling a set of street addresses across a range of demographic variables, and recording the menu items and number of available outlets offered to each address. Machine learning was utilised to evaluate the healthiness of menu items, and we examined if healthiness and the number of available outlets varied by neighbourhood demographics. Over 75% of menu items offered by all MDAs were unhealthy and approximately 30% of all menu items across the three MDAs scored at the lowest level of healthiness. Statistically significant differences by demographics were identified in one of the three MDAs in this study, which suggested that the proportion of unhealthy foods offered was highest in areas with the greatest socioeconomic deprivation and those with a higher proportion of Māori population. Policy and regulatory approaches need to adapt to this novel mode of access to unhealthy foods, to mitigate public health consequences and the effects on population groups already more vulnerable to non-communicable diseases.
Preoperative High Visceral Fat Increases Severe Complications but Improves Long-Term Prognosis after Gastrectomy for Patients with Advanced Gastric Cancer: A Propensity Score Matching Analysis
Authors: Matsui Ryota , Inaki Noriyuki , Tsuji Toshikatsu , Kokura Yoji , Momosaki Ryo
Published: October 2022

This study investigated the paradox of high visceral fat mass increasing severe complications but improving long-term prognosis after radical gastrectomy for gastric cancer. This was a retrospective cohort study of consecutive patients with primary stage I–III gastric cancer who underwent gastrectomy between April 2008 and June 2018. The visceral adipose tissue index (VAI) was calculated by dividing the visceral fat mass preoperatively measured on computed tomography by the square of the height. Patients with VAIs below the median cut-off value were classified as low-VAI, while those above it were classified as high-VAI. We compared the postoperative complication rate and overall survival (OS) in the low- and high-VAI groups after adjusting patient characteristics using propensity score matching (PSM). There were 155 patients in both groups after PSM. After matching, there was no significant difference in factors other than BMI and VAI that were not adjusted. The high-VAI group had more severe postoperative complications (p = 0.018), but the OS was significantly better in the high-VAI group (hazard ratio 0.611, 95%CI 0.403–0.928, p = 0.021). Preoperative high visceral fat mass not only increased severe complications, but also improved OS after gastrectomy in patients with advanced gastric cancer.
The Efficient Antiviral Response of A549 Cells Is Enhanced When Mitochondrial Respiration Is Promoted
Authors: Lebeau Grégorie , El Safadi Daed , Paulo-Ramos Aurélie , Hoareau Mathilde , Desprès Philippe , Krejbich-Trotot Pascale , Chouchou Florian , Roche Marjolaine , Viranaicken Wildriss
Published: October 2022

When exposed to a viral infection, the attacked cells promptly set up defense mechanisms. As part of the antiviral responses, the innate immune interferon pathway and associated interferon-stimulated genes notably allow the production of proteins bearing antiviral activity. Numerous viruses are able to evade the interferon response, highlighting the importance of controlling this pathway to ensure their efficient replication. Several viruses are also known to manipulate the metabolism of infected cells to optimize the availability of amino acids, nucleotides, and lipids. They then benefit from a reprogramming of the metabolism that favors glycolysis instead of mitochondrial respiration. Given the increasingly discussed crosstalk between metabolism and innate immunity, we wondered whether this switch from glycolysis to mitochondrial respiration would be beneficial or deleterious for an efficient antiviral response. We used a cell-based model of metabolic reprogramming. Interestingly, we showed that increased mitochondrial respiration was associated with an enhanced interferon response following polyriboinosinic:polyribocytidylic acid (poly:IC) stimulation. This suggests that during viral infection, the metabolic reprogramming towards glycolysis is also part of the virus’ strategies to inhibit the antiviral response.