bluuebunny/crossref_metadata_2025 huggingface dataset:
In vitro and in vivo studies on the bioactivity of a ginger (Zingiber officinale) extract towards adult schistosomes and their egg production
Authors: Sanderson L. , Bartlett A. , Whitfield P.J.
Published: February 2003

The bioactivity of an ethyl acetate extract of ginger (Zingiber officinale) towards Schistosoma mansoni adult pairs, both cultured in vitro and in vivo in laboratory mice, was investigated by monitoring worm mortality and fecundity. In vitro, a concentration of 200 mg l-1 of extract killed almost all worms within 24 h. Male worms seemed more susceptible than female under these conditions. Cumulative egg output of surviving worm pairs in vitro was considerably reduced when exposed to the extract. For example, after 4 days of exposure to 50 mg l-1, cumulative egg output was only 0.38 eggs per worm pair compared with 36.35 for untreated worms. In vivo efficacy of the extract was tested by oral and subcutaneous delivery of 150 mg kg-1 followed by assessment of worm survival and fecundity. Neither delivery route produced any significant reduction in worm numbers compared with untreated controls. Worm fecundity was assessed in vivo by cumulative egg counts per liver at 55 days post infection with mice treated subcutaneously. Such infections showed egg levels in the liver of about 2000 eggs per worm pair in 55 days, in both treated and control mice, with no significant difference between the two groups. To ensure that density-dependent effects did not confound this analysis, a separate experiment demonstrated no such influence on egg output per worm pair, at intensities between 1 and 23 worms per mouse.
Brachylaima cribbi (Digenea: Brachylaimidae): scanning electron microscopical observations of the life-cycle stages
Authors: Butcher A.R. , Brealey J.K. , Grove D.I. , Dymock R.B.
Published: February 2003

Brachylaima cribbi is a recently described species of terrestrial trematode that infects mammals and birds with helicid land snails as its first and second intermediate hosts. The adult worm is 2.5–6.0 mm long by 0.5–0.8 mm wide being a long slender cylindrical worm with oral and ventral suckers in the anterior quarter and genital pore in the posterior quarter. Scanning electron microscopy shows that there is a dense covering of tegumental spines at the anterior end which diminishes towards the posterior extremities of the worm. Development of spines was observed in juvenile and mature adult worms. In young worms 1–3 weeks post infection (wpi) spines appear as buds with a serrated edge each having 1–4 spikes per spine. As the worm ages the spines broaden and by 5 wpi the number of spikes per spine increases to an average of 8.1. The serial development of oral sucker papillae in the cercaria, metacercaria and adult worm was observed with the finding of an elongated papilla with a bifurcated tip on the cercaria becoming a shorter and thicker elongated papilla with a large central stoma on the metacercaria. In the adult worm, this papilla becomes dome-shaped with a small central stoma. For some of these papillae a cilium could be seen extended from the central stoma. Other life-cycle stages illustrated were the hatched egg with an extruded egg membrane minus an operculum and a portion of the branched sporocyst dissected from the digestive gland of the land snail Theba pisana showing a terminal birth pore. Scanning electron microscopy morphological features of the adult worm observed for the first time in a Brachylaima were the unarmed cirrus extended from the genital pore with released sperm present and the Laurer's canal opening visible in tegumental folds on the dorsal surface approximately 300 μm posterior to the genital pore.
Molecular evidence for the synonymy of two species of Apatemon Szidat, 1928, A. gracilis (Rudolphi, 1819) and A. annuligerum () (Digenea: Strigeidae) parasitic as metacercariae in British fishes
Authors: Bell A.S. , Sommerville C.
Published: February 2003

Tenascin is an extracellular matrix glycoprotein known to be an essential factor for the modulation of reciprocal interactions between the epithelium and mesenchyme during embryogenesis and tumourigenesis. The interactions between the expression of tenascin in the liver of Syrian golden hamster and the development of bile duct cancer in an Opisthorchis viverrini-associated cholangiocarcinoma model were investigated. The tenascin was expressed in connective tissues surrounding the dilated ducts, ductal rims and the stroma of cancers, and strongly in the stroma flame of necrotic cancer nodules. The mRNA signal for tenascin was also recognized in the stroma cells. The potential roles of tenascin as prognostic tumour markers are discussed.
Emergence of cercariae of Echinostoma caproni and Schistosoma mansoni from Biomphalaria glabrata under different laboratory conditions
Authors: Fried B. , Laterra R. , Kim Y.
Published: February 2003

Release of Echinostoma caproni cercariae and Schistosoma mansoni from experimentally infected Biomphalaria glabrata snails maintained under different laboratory conditions was studied. Infected snails were isolated individually for 1 h in Stender dishes containing 5 ml of artificial spring water and the number of cercariae released during this time was recorded. Of numerous conditions tested, the addition of lettuce, the use of water conditioned by B. glabrata snails and a temperature of 35°C significantly increased the release of E. caproni cercariae. A significant increase in cercarial release of S. mansoni was seen only in cultures fed lettuce. A temperature of 12°C caused a significant decrease in cercarial release of both E. caproni and S. mansoni. Increased snail activity associated with feeding behaviour was probably responsible for the enhanced cercarial sheds observed in this study.
Daily emergence of Schistosoma mansoni and S. haematobium cercariae from naturally infected snails under field conditions
Authors: Wolmarans C.T. , de Kock K.N. , Strauss H.D. , Bornman M.
Published: February 2003

The daily emergence of Schistosoma mansoni and S. haematobium cercariae was investigated under field conditions. Intermediate host snails of both schistosome species were collected during the rainy season, cold dry season and warm dry season and kept separately in test tubes in habitat water. Shed cercariae were collected from each of the test tubes at two hourly intervals, transferred to Petri dishes and counted. Mice were exposed to these cercariae to establish the identity of the schistosome parasites. Peak shedding for both species was observed at 1100 h during the rainy and warm dry seasons and at 0900 h during the cold dry season. Shedding before 0900 h was found only for S. haematobium in the rainy season while shedding after 1700 h occurred only during this season at both species. Shedding observed during 1900 h observation period was in the low category for both species. No shedding was observed during the 2100 h observation period for any of the species and the investigation was discontinued after this period. Only S. haematobium ova were found in the exposed mice.
Dynamics of Anguillicola crassus larval infections in a paratenic host, the ruffe (Gymnocephalus cernuus) from the Oder River on the border of Germany and Poland
Authors: Pietrock M. , Meinelt T.
Published: February 2003

The course of infection of 2-year-old ruffe (Gymnocephalus cernuus L.) from the Oder River (Germany/Poland) with third-stage larvae (L3) of Anguillicola crassus (Nematoda: Anguillicolidae) was investigated at monthly intervals between March 1994 and March 1995. Of 230 fish examined, 152 (66%) harboured viable L3. Monthly prevalences fluctuated between 12 and 96%; mean intensities ranged from 1.0 to 5.6 L3 per infected fish. Temperature-dependent differences in infection were noted with the prevalence and mean intensity of infection being significantly higher (Chi-Square test and Mann-Whitney-U-test, respectively; P<0.01) at water temperatures less than 10°C (cold period) than at those above (warm period). These differences remained significant even after the division of fish samples into two size classes (8.5–10.5 cm, 11–13 cm). Although the paratenic hosts may acquire infections year round, the results suggest that ruffe become infected mainly during the cold season and that the host's feeding ecology determines the final course of infection.
Do helminths increase the vulnerability of released pheasants to fox predation?
Authors: Millán J. , Gortázar C. , Tizzani P. , Buenestado F.J.
Published: February 2003

The success of ring-necked pheasant (Phasianus colchicus) restocking in Asturias, northern Spain was assessed, and the role of parasites and predators in the mortality of released birds was studied. The experimental release of 56 radio-tagged pheasants showed that 98% of birds died within 12 days. As soon as 72 h after release, 67.5% of males and 55.0% of females were found dead. Foxes (Vulpes vulpes) killed 63% of the birds. The survival of those birds killed by foxes was lower than for birds which died due to other causes, and pheasants depositing eggs of the nematode Eucoleus contortus (Creplin, 1839) survived less than those apparently non-parasitized. No impact of the parasite on the pheasants' condition was found, but foxes preyed upon parasitized birds more than expected by random. The results suggest that: (i) the current pheasant releases in this area are unsuccessful and need to be improved; (ii) this is mainly due to intense predation by red foxes; and (iii) parasites could have some influence on the predation of released birds by foxes. However, the way parasites affect pheasant vulnerability remains unclear.
Life cycle of Trichostrongylus retortaeformis in its natural host, the rabbit (Oryctolagus cuniculus)
Authors: Audebert F. , Hoste H. , Durette-Desset M.C.
Published: February 2003

The chronology of the life cycle of Trichostrongylus retortaeformis (Zeder, 1800) (Nematoda, Trichostrongyloidea) is studied in its natural host Oryctolagus cuniculus. The free living period lasted 5 days at 24°C. Worm-free rabbits were each infected per os with T. retortaeformis larvae. Rabbits were killed at 12 h post-infection (p.i.) and every day from one day to 13 days p.i. By 12 h p.i., all the larvae were exsheathed and in the small intestine. The third moult occurred between 3 and 5 days p.i. and the last moult between 4 and 7 days p.i. The prepatent period lasted 12 to 13 days. The patent period lasted five and a half months. The four known life cycles of species of Trichostrongylus in ruminants were compared with that of T. retortaeformis. No significant difference was found except in the duration of the prepatent period. These similarities in the life cycles confirm the previously formulated hypotheses on the relationship between the parasites of the two host groups ().
Expression of Hsp90, Hsp70 and Hsp60 in Trichinella species exposed to oxidative shock
Authors: Martinez J. , Perez-Serrano J. , Bernadina W.E. , Rodriguez-Caabeiro F.
Published: February 2003

Stress response and phosphorylation of heat shock proteins (HSPs) 60, 70 and 90 were studied in Trichinella nativa, T. nelsoni, T. pseudospiralis and T. spiralis larvae at 30-min intervals following exposure to 20, 100 and 200 mM H2O2. There was a time- and dose-dependent differential survival for the infective stage larvae (L1) of these four Trichinella species. Immunoblotting analysis revealed that constitutive Hsp60 and Hsp70, but not Hsp90, from test Trichinella species are constitutively phosphorylated on serine/threonine residues as they converted to forms with increased sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) mobility by treatment with alkaline phosphatase. After exposure to H2O2, while there was a time-related occurrence of the three HSPs with decreased SDS–PAGE mobility, these HSPs were insensitive to alkaline phosphatase except in the case of exposure to 20 mM H2O2 for Hsp60 from all Trichinella species and Hsp70 from T. spiralis and T. nelsoni. The synthesis of HSPs forms with decreased SDS–PAGE mobility is a susceptibility signal because the lower concentration of peroxide (20 mM) did not cause a decrease on HSPs SDS–PAGE mobility in T. spiralis and T. nelsoni, the two more resistant selected Trichinella species.
Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis
Authors: Ito A. , Putra M.I. , Subahar R. , Sato M.O. , Okamoto M. , Sako Y. , Nakao M. , Yamasaki H. , Nakaya K. , Craig P.S. , Margono S.S.
Published: February 2003

Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.
Expression of tenascin in bile duct cancer of hamster liver by combined treatment of dimethylnitrosamine with Opisthorchis viverrini infections
Authors: Sithithaworn P. , Ando K. , Limviroj W. , Tesana S. , Pairojkul C. , Yutanawiboonchai W. , Chinzei Y. , Yoshida T. , Sakakura T.
Published: February 2003

Tenascin is an extracellular matrix glycoprotein known to be an essential factor for the modulation of reciprocal interactions between the epithelium and mesenchyme during embryogenesis and tumourigenesis. The interactions between the expression of tenascin in the liver of Syrian golden hamster and the development of bile duct cancer in an Opisthorchis viverrini-associated cholangiocarcinoma model were investigated. The tenascin was expressed in connective tissues surrounding the dilated ducts, ductal rims and the stroma of cancers, and strongly in the stroma flame of necrotic cancer nodules. The mRNA signal for tenascin was also recognized in the stroma cells. The potential roles of tenascin as prognostic tumour markers are discussed.
Predacious activity of Duddingtonia flagrans within the cattle faecal pat
Authors: Faedo M. , Larsen M. , Grønvold J.
Published: February 2003

Two studies were conducted to investigate the growth and activity of the fungus, Duddingtonia flagrans, within cattle faecal pats. Artificial faecal pats were constructed with the centre separated from the outer layer by a nylon mesh. Eight treatments were tested, by varying the presence/absence of Cooperia oncophora eggs and fungal spores within each layer. With parasite eggs in the centre layer, a statistically lower recovery of larvae was observed compared to both pats with parasite eggs in the periphery and pats with parasite eggs throughout both layers. Regardless of location within the pat, if co-located with the parasite egg, D. flagrans was found to be effective in trapping developing larvae. The reduction in recovery of larvae from pats with parasite eggs and fungal spores in the centre was found to be significantly higher than when parasite eggs were in the centre and fungal spores in the periphery. In the second study, pats were made up in two treatments: pats containing fungal spores and C. oncophora eggs (fungus) and pats containing C. oncophora eggs (control). The pats were incubated at low or high humidity. Ten pats were used in a cross over where five pats incubated at low humidity for 7 weeks were removed, water added and then incubated at a high humidity for 1 week. Another five pats were incubated at a high humidity for 7 weeks, aerated and incubated at a low humidity for 1 week. There was no apparent growth of fungus in faecal pats incubated at a high humidity and less than 20% of larvae were recovered. The growth of D. flagrans was observed in faecal pats incubated at a low humidity, but a corresponding reduction in the percentage recovery of larvae did not occur, except in week 4. No statistical difference between fungal and control pats was seen in the change over pats. Nematophagous activity was assessed throughout the study and observed in the first 4 weeks within the pats containing fungus.
The effect of cobalt compounds on uninfected and Ascaridia galli-infected chickens: a kinetic model for Ascaridia galli populations and chicken growth
Authors: Gabrashanska M. , Teodorova S.E. , Mitov M.
Published: February 2003

The effect of dietary cobalt from three different sources on uninfected and Ascaridia galli-infected Hisex chickens, has been studied. The chicken diet was supplemented with 0.06 Co2+ kg-1 food either in the form of two glycine–cobalt compounds or mixed zinc–cobalt basic salt. An excess of dietary cobalt in small doses increases the gain of body weight and decreases host mortality. A greater bioefficiency of cobalt was established in infected chickens. A mathematical model has been used to provide a quantitative interpretation of the observed results. The model solutions of the kinetics of worm numbers and body weight are in a good agreement with experimental data. The model is valid for different degrees of A. galli infections and for treatment with different trace elements. The value of the kinetic parameter, regarded as a phenomenological constant of the host immune response, depends on the degree of infection.
Abnormalities of the attachment clamps of representatives of the family Diplozoidae
Authors: Šebelová Š. , Kuperman B. , Gelnar M.
Published: February 2003

A comparative study has been made of the haptoral morphology of four species of diplozoon (Monogenea: Diplozoidae) from the gills of fish exposed to different levels of water pollution in two river systems in eastern Europe. An examination of the haptors of Paradiplozoon homoion (Bychowsky & Nagibina 1959), Paradiplozoon ergensi (Pejčoch 1968) and Paradiplozoon megan (Bychowsky & Nagibina 1959) from chub caught in the River Morava, Czech Republic and of Diplozoon paradoxum (Nordmann 1832) from bream recovered from the River Volga, Russia has revealed abnormalities to the attachment clamps. Two abnormal conditions were found: structural alterations to the attachment clamps and changes in the number of attachment clamps; these occurred both singly and in combination. A higher frequency of abnormal attachment clamps was found in diplozoons from fish caught in the more polluted localities of both rivers. The abnormalities have been recorded and their morphology compared in the light of conditions of environmental stress.
Adult worm tegumental damage and egg-granulomas in praziquantel-resistant and -susceptible Schistosoma mansoni treated in vivo
Authors: Liang Y.-S. , Coles G.C. , Dai J.-R. , Zhu Y.-C. , Doenhoff M.J.
Published: February 2003

The effect of treatment with praziquantel (PZQ) on the tegument of adult Schistosoma mansoni worms and on liver egg-granulomas has been examined in mice infected with PZQ-resistant and -susceptible parasite isolates. Two PZQ-resistant S. mansoni isolates, one selected by passage in the laboratory under drug pressure and one from Senegal established from eggs excreted by an uncured patient, were compared with PZQ-susceptible control isolates. Scanning electron microscopic observations on the tegument of Schistosoma adult worms treated in vivo with PZQ showed that more severe damage was inflicted by PZQ on susceptible worms than on drug-resistant worms. Observations on the pathology of Schistosoma egg-granulomas in the liver of infected mice after treatment with PZQ indicated that eggs from susceptible control isolates were more sensitive to PZQ than those from drug-resistant isolates.
The Hydrobia ulvae–Maritrema subdolum association: influence of temperature, salinity, light, water-pressure and secondary host exudates on cercarial emergence and longevity
Authors: Mouritsen K.N.
Published: February 2003

The effects of environmental factors and exudates from the amphipod Corophium volutator on the emergence of Maritrema subdolum cercariae (Digenea: Microphallidae) from the snail Hydrobia ulvae were investigated in the laboratory. Increasing the temperature (15 to 25°C) caused an overall 11-fold increase in emergence rate under varying salinities (24 to 36‰). The effect of salinity depended on the experimental temperature. Emergence increased with increasing salinity at higher temperatures, but decreased with increasing salinity at 15°C.Whereas the different levels of salinity had no effect, increasing the temperature significantly reduced the life span of cercariae. In comparison with complete darkness, light caused a two-fold increase in emergence, whereas an increment of the water pressure from 1.0 to 1.3 ATM (corresponding to 0 and 3 m of depth) left the shedding rate unaffected. Unidentified exudates from the second intermediate host, C. volutator, significantly depressed the cercarial emergence rate. The main transmission window of M. subdolum seems to occur during low water in tidal pools where light levels are high and solar radiation rapidly elevates the water temperature, as well as salinity through evaporation. The consequence of such a transmission strategy is discussed in relation to the impact of M. subdolum on the population dynamics of the second intermediate host.
The Hydrobia ulvae–Maritrema subdolum association: cercarial emergence controlled by host activity
Authors: Mouritsen K.N.
Published: February 2003

The release of Maritrema subdolum cercariae (Digenea: Microphallidae) from the marine mud snail Hydrobia ulvae is significantly affected by temperature, salinity, light and exudates from the second intermediate amphipod host. Based on (i) previously published data on temperature–salinity dependent H. ulvae activity, (ii) new experimental data on H. ulvae activity in light and darkness as well as in the presence and absence of host exudates, and (iii) the cercarial emergence rate from free moving snails and snails prevented from crawling, the present analysis indicates that emergence of M. subdolum larvae is regulated mainly by host activity as the ultimate factor for release. The adaptive significance of such an emergence strategy is emphasized.
Qualitative and quantitative aspects of recent research on helminth parasites
Authors: Poulin R.
Published: February 2003

Relationships between the species diversity of different taxa, the mean number of articles published per year on each taxon, and the mean impact factor of the journals in which they appear, were examined across six taxa of helminths: Nematomorpha, Acanthocephala, Monogenea, Trematoda, Cestoda and Nematoda, the latter including only animal parasitic nematodes. The mean annual output of scientific articles per taxon was not related to the species diversity of these taxa or, at least, not significantly. Thus, the large volume of publications on nematodes is not merely a reflection of their estimated diversity. There were significant differences among taxa in the mean impact factor of the journals in which papers on each taxon appeared, with nematodes having the highest mean score, followed by trematodes and cestodes. In addition, across the six taxa, the mean journal impact factor correlated positively and significantly with the mean annual number of papers published: not only are there more papers published on nematodes and trematodes than on nematomorphs or acanthocephalans, but they are also generally published in higher-ranking journals. These results suggest that there is an increasing gap in the quantity and general importance of the research carried out on different helminth taxa.
Immunodiagnosis of ocular toxocariasis using Western-blot for the detection of specific anti-Toxocara IgG and CAP™ for the measurement of specific anti-Toxocara IgE
Authors: Magnaval J.-F. , Malard L. , Morassin B. , Fabre R.
Published: February 2003

A prospective multicentric study was carried out to assess both the performance of Western-blot (WB) detecting specific anti-Toxocara IgG and that of CAP™ measuring specific IgE titre for the immunodiagnosis of ocular toxocariasis. For 14 outpatients presenting ophthalmic symptoms (choroiditis, chorioretinitis, papillar oedema, hyalitis, retinal detachment and/or uveitis), samples of serum and aqueous fluid (AF) were sent to the Department of Parasitology, University Hospitals, Toulouse, France. All patients but two tested positive with WB on the serum; 13 WB tests were performed on the AF, 12 of which were positive. The two patients who had a negative WB serum result tested positive for the AF. Specific IgE detection was considered as a complementary test of WB. Two patients showed a greater specific IgE titre in the AF than in the serum, and one had a positive result in the AF, but not in the serum. These six patients were considered as clear cases of ocular toxocariasis. Western-blot coupled with specific anti-Toxocara IgE detection appeared therefore to be an accurate procedure for the immunodiagnosis of ocular toxocariasis, provided the testing was simultaneously performed on the serum and AF.
The response of Anisakis larvae to freezing
Authors: Wharton D.A. , Aalders O.
Published: February 2003

Anisakis third stage larvae utilize a variety of fish as intermediate hosts. Uncooked fish are rendered safe for human consumption by freezing. Larvae freeze by inoculative freezing from the surrounding medium but can survive freezing at temperatures down to -10°C. This ability may be aided by the production of trehalose, which can act as a cryoprotectant, but does not involve recrystallization inhibition. Monitoring of fish freezing in commercial blast freezers and under conditions which simulate those of a domestic freezer, indicate that it can take a long time for all parts of the fish to reach a temperature that will kill the larvae. This, and the moderate freezing tolerance of larvae, emphasizes the need for fish to be frozen at a low enough temperature and for a sufficient time to ensure that fish are safe for consumption.
Endoparasites of three species of house geckoes in Lampung, Indonesia
Authors: Matsuo K. , Oku Y.
Published: February 2003

Thirty-four house geckoes (21 Cosymbotus platyurus, four Gehyra mutilata and nine Hemidactylus frenatus) in December, 1997 (rainy season) and 26 geckos (16 C. platyurus, six G. mutilata and four H. frenatus) in July, 2000 (dry season) were captured in Lampung, Indonesia. Some species of geckoes have been inadvertently introduced to many tropical regions from their native region, but the three species of geckoes in this study are native to Indonesia. Six species of endoparasites were recovered: Oochoristica javanensis (Cestoda) from the small intestine, Paradistomum geckonum (Digenea) from the small intestine and gallbladder, Postorchigenes ovatus (Digenea) from the small intestine, Spauligodon hemidactylus (Nematoda) from the large intestine, and Raillietiella gehyra and R. frenatus (Pentastomida) from the lungs. The prevalence and mean intensity of infection in each species of geckoes are also presented. The prevalence of S. hemidactylus from C. platyurus and H. frenatus in the rainy season was significantly higher than in the dry season. The low prevalence of S. hemidactylus in G. mutilata in the present study corresponded to a previous report from a non-native area. According to the original description, the male of S. hemidactylus lacked a spicule, but in the present study, one male was found with a spicule. The present study suggests male dimorphism occurs in Spauligodon. The number of endoparasite species and snout vent length of geckoes were positively correlated. Geckoes with high worm burdens may be more easily captured by predators.
The North/South Ireland Food Consumption Survey: survey design and methodology
Authors: Harrington KE , Robson PJ , Kiely M , Livingstone MBE , Lambe J , Gibney MJ
Published: February 2003

Objective

The purpose of this survey was to establish a database of habitual food and drink consumption in a representative sample of Irish adults aged 18-64 years.

Design

A cross-sectional food consumption survey was carried out. Food intake data were collected using a 7-day estimated food diary. Anthropometric data included measurements of weight, height, waist and hip circumferences and body composition using bioelectrical impedance analysis. Questionnaire data included assessments of health, lifestyle and socio-demographic status, levels of physical activity, attitudes to diet and health and restrained eating.

Setting

Northern Ireland and Republic of Ireland between 1997 and 1999.

Results

In total, 1379 adults aged 18–64 years participated in the survey and completed the 7-day food diary. This paper gives details of the methods used to carry out the survey. Sampling, respondent recruitment, dietary assessment, collection of anthropometric and questionnaire data and data management and analysis are described.

Conclusion

The North/South Ireland Food Consumption Survey is unique in being the first food consumption survey ever to be carried out in the Republic of Ireland and Northern Ireland concurrently using the same methodology.
An evaluation of energy intakes and the ratio of energy intake to estimated basal metabolic rate (EI/BMRest) in the North/South Ireland Food Consumption Survey
Authors: McGowan MJ , Harrington KE , Kiely M , Robson PJ , Livingstone MBE , Gibney MJ
Published: February 2003

Objective

To examine energy intakes (El), their ratio to estimated basal metabolic rate (BMRest) and the contribution of food groups to energy intake in the North/South Ireland Food Consumption Survey.

Design and setting

Random sample of adults from the populations of Northern Ireland and the Republic of Ireland. Food intake data were collected using a 7-day food diary. Body weight and height were measured and EI/BMRest was calculated from reported energy intake and estimated basal metabolic rate. Dieting practices were assessed as part of a self-administered questionnaire.

Results

Mean energy intake in men was 11.0 MJ and in women was 7.6 MJ, which is comparable to reported energy intakes in Northern Ireland and the Republic of Ireland over a decade ago. Mean EI/BMRest was 1.38. This increased to 1.42 after the exclusion of dieters and those who were unwell, but still remained less than the established cut-off of 1.53. EI/BMRest was significantly (P < 0.05) higher in men than in women and decreased significantly (P < 0.05) with increasing BMI in both sexes. The four food groups that contributed 50% of energy in men and women were meat and meat products, breads and rolls, potatoes and potato products, and biscuits, cakes, pastries and puddings.

Conclusions

Energy intakes have not changed remarkably in Northern Ireland or the Republic of Ireland in the last 10 years, but the mean EI/BMRest of 1.38 suggests that energy underreporting occurred. EI/BMRest was lower in women and in the overweight/obese. Additional multivariate analysis of the data is needed to identify more clearly subgroups of the population reporting lower than expected energy intakes and to evaluate the effect of low energy reporting on the consumption of various foods and food groups.
Macronutrient intakes and food sources in Irish adults: findings of the North/South Ireland Food Consumption Survey
Authors: Harrington KE , McGowan MJ , Kiely M , Robson PJ , Livingstone MBE , Morrissey PA , Gibney MJ
Published: February 2003

Objective

To describe macronutrient intakes and food sources of the adult population in the Republic of Ireland and Northern Ireland and to assess adherence of this population to current dietary recommendations.

Design

A cross-sectional food consumption survey collected food intake data using a 7-day food diary.

Setting

Northern Ireland and the Republic of Ireland between October 1997 and October 1999.

Subjects

One thousand three hundred and seventy-nine adults aged 18–64 years (662 males and 717 females).

Results

Mean daily energy intakes in men were 11 MJ per day, 15.5% was derived from protein, 34.8% from fat, 43.5% from carbohydrate and 5.9% from alcohol. Corresponding figures for women were 7.6 MJ per day, 15.6%, 35.6%, 45.1% and 3.5%. When alcohol energy was excluded the contribution of fat and carbohydrate to energy did not differ between men and women. When compared with existing dietary recommendations, 93% of men and 86% of women had protein intakes above the Population Reference Intake. Two approaches were used to assess adherence to the fat and carbohydrate dietary recommendations: (1) the proportion of individuals in the population attaining these dietary targets and (2) the proportion of the population that was included in a 'compliers' group which had a group mean equal to these dietary targets. Thirty-three per cent of men and 34% of women met the target of 35% of food energy from fat and 78% of men and 80% of women comprised the ‘compliers’ group having a group mean of 35% of food energy from fat. Twentythree per cent of men and 27% of women met the target of 50% of food energy from carbohydrate and 56% of men and 62% of women made up the 'compliers' group. Meat and meat products were the main source of fat (23%) and protein (37%), and bread and rolls (25%) were the main source of carbohydrate.

Conclusion

A reduction in dietary fat intake remains an important public health issue in the Republic of Ireland and Northern Ireland. An increase in carbohydrate intake and attention to the rise in alcohol intake is also warranted.
The North/South Ireland Food Consumption Survey: the dietary fibre intake of Irish adults
Authors: Galvin MA , Kiely M , Harrington KE , Robson PJ , Moore R , Flynn A
Published: February 2003

Objective

Mean daily intakes of dietary fibre (DF, Southgate) and non-starch polysaccharide (NSP, Englyst) are estimated in a representative sample of adults aged 18-64 years in Ireland. The contribution of food groups to DF and NSP intake is reported and fibre intakes are compared with dietary recommendations and with intakes in some European countries.

Design

Food consumption was estimated using 7-day food diaries for a representative sample (n = 1379; 662 men, 717 women) of 18–64 year old adults in the Republic of Ireland and Northern Ireland selected from the electoral register. DF and NSP intakes were estimated from tables of food composition.

Results

The mean daily intake of DF in the total sample was 20.2 g (standard deviation (SD) 7.8) [23-2 g (SD 8.5) in men, 17.4 g (SD 5.9) in women] or 2.24 g MJ−1 (SD 0.7) [2.16 g MJ−1 (SD 0.7) in men, 2.33 g MJ−1 (SD 0.7) in women]. Mean daily intake of NSP was 14.8 g (SD 6.2) [16.7 g (SD 6.8) in men, 13.0 g (SD 5.0) in women] or 1.65 g MJ−1 (SD 0.6) [1.56 g MJ−1 (SD 0.6) in men, 1.74 g MJ−1 (SD 0.6) in women]. The main food groups that contributed to mean daily intake of DF (NSP) in the sample were breads 31% (23%), potatoes 19% (23%), and vegetables 17% (19%). Absolute intakes of DF and NSP were higher (P < 0.001) in men than women; however, women overall consumed more (P < 0.001) fibre-dense diets than men. Women aged 18-35 years consumed less (P < 0.01) DF and NSP (g) than women aged 36-64 years. Both men and women aged 18–35 years consumed less (P < 0.01) fibre-dense diets than men and women aged 36–64 years. The NSP intake was below the nutritional goal of 18 g day−1 in 77% of adults and below the minimum of the recommended range (12 g day−1) in 37% of the total sample. Compliance (i.e. the maximum number of individuals whose collective mean daily intake corresponded to the population goal) with the (UK) population goal for an average intake of 18 g day−1 NSP was achieved by a greater proportion of the population (63%) than compliance with the (German) dietary fibre recommendation of 30 g day−1 (27%) or the (Nordic) recommendation of 3 g MJ−1 day−1 (33%).

Conclusion

DF (NSP) intakes were lower than dietary recommendations in a substantial proportion of the population. This is likely to contribute to impaired bowel function and constipation, which in turn may contribute to increased risk of chronic gastrointestinal disease.
Physical activity patterns in a nationally representative sample of adults in Ireland
Authors: Livingstone MBE , Robson PJ , McCarthy S , Kiely M , Harrington K , Browne P , Galvin M , Wareham NJ , Rennie KL
Published: February 2003

Objective

To evaluate habitual levels of physical activity in a nationally representative sample of adults in Ireland.

Design

Cross-sectional survey using a self-administered questionnaire. Usual levels of work, recreational and household activities were evaluated in relation to anthropometric, demographic and socio-economic characteristics. The amount and intensity of all activities were quantified by assigning metabolic equivalents (METS) to each activity.

Setting

Republic of Ireland and Northern Ireland, 1997–1999.

Subjects

Random sample of 1379 adults aged 18–64 years.

Results

Men were approximately twice as active in work and recreational activity (139.7 ± 83.9 METS) as women (68.5 ± 49.8 METS; P < 0.001), but women were three times more active in household tasks (65.9 ± 58.7 METS vs. 22.6 ± 24.6 METS; P < 0.001). Overall levels of physical activity declined with increasing age, particularly leisure activity in men. In women the decline in work activity was offset by spending more time in household pursuits. Twenty-five per cent of the subjects were extremely overweight (body mass index (BMI) > 28kg m−2) or obese (BMI > 30kg m−2). Fewer obese subjects reported higher levels of work and leisure activities. However, a higher percentage of obese women reported participation in the higher levels of household activities. Participation rates in recreational activities were low. Walking was the most important leisure activity of both men (41%) and women (60%). In terms of hours per week spent in vigorous physical activity, men were more active than women, professional and skilled non-manual women were more active than women in other social classes, and younger subjects (aged 18–35 years) were more active than older subjects.

Conclusions

The holistic approach used in the assessment of physical activity in this study has revealed important and subtle differences in the activity patterns of men and women. Failure to fully characterise the respective activity patterns of men and women could lead to ill-informed public health policy aimed at promoting and sustaining lifetime habits of physical activity. The results suggest that simple population-focused programmes to promote physical activity are unlikely to offer the same chance of long-term success as more sensitive and individualised strategies.
Attitudes towards and beliefs about nutrition and health among a random sample of adults in the Republic of Ireland and Northern Ireland
Authors: Kearney John M , Gibney Michael J , Livingstone Barbara E , Robson Paula J , Kiely Mairead , Harrington Karen
Published: February 2003

Objectives

For effective healthy eating promotion, it is necessary to understand the attitudes towards and beliefs about nutrition of the general public. The objective of this study was to provide data on attitudes towards eating a healthy diet and the perceived need to alter eating habits from a random sample of adults in the Republic of Ireland and in Northern Ireland, using a self-administered questionnaire.

Design

Cross-sectional survey using a self-administered attitudinal questionnaire on beliefs and attitudes to healthy eating.

Setting

The survey was carried out between October 1997 and October 1999 in the Republic of Ireland and in Northern Ireland.

Subjects

A randomly selected sample of 1256 adults from the Republic of Ireland and Northern Ireland completed the attitudinal questionnaire.

Results

A majority of subjects (62%) perceived that they make conscious efforts to eat a healthy diet either most of the time or quite often, while just over half (52%) agreed that they do not need to make changes to their diet as it is healthy enough. Subjects most likely to make conscious efforts to try to eat a healthy diet were females, older subjects (51-64 years) and those with the highest intakes of fruit and vegetables and lowest quartile of fat (% food energy). When self-assessed adequacy of fruit and vegetables was examined, two-thirds of the total sample felt they ate too little fruit while just one-third felt they ate too little vegetables.

Conclusion

Results of the present study, in general, revealed good agreement between attitude and behaviour with respect to healthy eating. This suggests that people appear to be reasonably accurate at evaluating their own diet in terms of how healthy it is. In terms of the two food groups examined in this study, some optimistic bias was evident for vegetables but not for fruit. It may be useful therefore to assess attitudes and beliefs about healthy eating by way of examining attitudes to such food groups individually.
The impact of a nutrition education programme on the anthropometric nutritional status of low-income children in South Africa
Authors: Walsh CM , Dannhauser A , Joubert G
Published: February 2003

Objective:

The study determined the impact of a community-based nutrition education programme, using trained community nutrition advisors, on the anthropometric nutritional status of mixed-race children aged between 2 and 5 years.

Design and setting:

The programme was implemented over two years in four study areas in the Free State and Northern Cape Provinces. Two control areas were included to differentiate between the effect of the education programme and a food aid programme that were implemented simultaneously. Weight-for-age, height-for-age and weight-for-height were summarised using standard deviations from the NCHS reference median. For each of the indicators, the difference in the percentage of children below minus two standard deviations from the reference NCHS median in the initial and follow-up surveys was determined.

Subjects:

Initially 536 children were measured and, after two years of intervention, 815.

Results:

Weight-for-age improved in all areas, but only significantly in boys and girls in the urban study area, and in boys in one rural study area. No significant improvement in height-for-age occurred in any area. Weight-for-height improved significantly in the urban study area.

Conclusion:

The education programme in combination with food aid succeeded in improving the weight status of children, but was unable to facilitate catch-up growth in stunted children after two years of intervention.
Relationships between nutrition screening checklists and the health and well-being of older Australian women
Authors: Patterson Amanda J , Young Anne F , Powers Jennifer R , Brown Wendy J , Byles Julie E
Published: February 2003

Objectives:

To examine associations between nutrition screening checklists and the health of older women.

Design:

Cross-sectional postal survey including measures of health and health service utilisation, as well as the Australian Nutrition Screening Initiative (ANSI), adapted from the Nutrition Screening Initiative (NSI).

Setting:

Australia, 1996.

Subjects:

In total, 12 939 women aged 70–75 years randomly selected as part of the Australian Longitudinal Study on Women's Health.

Results:

Responses to individual items in the ANSI checklist, and ANSI and NSI scores, were associated with measures of health and health service utilisation. Women with high ANSI and NSI scores had poorer physical and mental health, higher health care utilisation and were less likely to be in the acceptable weight range. The performance of an unweighted score (TSI) was also examined and showed similar results. Whereas ANSI classified 30% of the women as ‘high-risk’, only 13% and 12% were classified as ‘high-risk’ by the NSI and TSI, respectively. However, for identifying women with body mass index outside the acceptable range, sensitivity, specificity and positive predictive values for all of these checklists were less than 60%.

Conclusions:

Higher scores on both the ANSI and NSI are associated with poorer health. The simpler unweighted method of scoring the ANSI (TSI) showed better discrimination for the identification of ‘at risk’ women than the weighted ANSI method. The predictive value of individual items and the checklist scores need to be examined longitudinally.
Vitamins in Spanish food patterns: The eVe Study
Authors: Aranceta Javier , Serra-Majem Lluís , Pérez-Rodrigo Carmen , Llopis Juan , Mataix José , Ribas Lourdes , Tojo Rafael , Tur Josep A
Published: February 2003

Objective:

To describe vitamin intakes in Spanish food patterns, identify groups at risk for inadequacy and determine conditioning factors that may influence this situation.

Design:

Pooled-analysis of eight cross-sectional regional nutrition surveys.

Subjects:

Ten thousand two hundred and eight free-living subjects (4728 men, 5480 women) aged 25–60 years. Respondents of population nutritional surveys carried out in eight Spanish regions (Alicante, Andalucía, Balearic Islands, Canary Islands, Catalunya, Galicia, Madrid and Basque Country) from 1990 to 1998. The samples were pooled together and weighted to build a national random sample.

Methods:

Dietary assessment by means of repeated 24-hour recall using photograph models to estimate portion size. Adjusted data for intra-individual variation were used to estimate the prevalence of inadequate intake. A Diet Quality Score (DQS) was computed considering the risk for inadequate intake for folate, vitamin C, vitamin A and vitamin E. DQS scores vary between 0 (good) and 4 (very poor). Influence of lifestyle (smoking, alcohol consumption and physical activity) was considered as well.

Results:

Inadequate intakes (<⅔ Recommended Dietary Intake) were estimated in more than 10% of the sample for riboflavin (in men), folate (in women), vitamin C, vitamin A, vitamin D and vitamin E. More than 35% of the sample had diets classified as poor quality or very poor quality. Factors identified to have an influence on a poor-quality diet were old age, low education level and low socio-economical level. A sedentary lifestyle, smoking, usual consumption of alcohol and being overweight were conditioning factors for a poor-quality diet as well.

Conclusion:

Results from The eVe Study suggest that a high proportion of the Spanish population has inadequate intakes for at least one nutrient and nearly 50% should adjust their usual food pattern towards a more nutrient-dense, healthier diet.