bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Direct-Fed Microbials on In-Vitro Rumen Fermentation of Grass or Maize Silage
Authors: Dhakal Rajan , Copani Giuseppe , Cappellozza Bruno Ieda , Milora Nina , Hansen Hanne Helene
Published: April 2023

Direct-fed microbial products (DFM) are probiotics that can be used advantageously in ruminant production. The in vitro gas production technique (IVGPT) is a method to simulate rumen fermentation and can be used to measure degradation, gas production, and products of fermentation of such additives. However, inter-laboratory differences have been reported. Therefore, tests using the same material were used to validate laboratory reproducibility. The objective of this study was to assess the effect of adding two DFM formulations on fermentation kinetics, methane (CH4) production, and feed degradation in two different basal feeds while validating a newly established IVGPT laboratory. Six treatments, with three replicates each, were tested simultaneously at the established IVGPT lab at the University of Copenhagen, and the new IVGPT lab at Chr. Hansen Laboratories. Maize silage (MS) and grass silage (GS) were fermented with and without the following DFM: P1: Ligilactobacillus animalis and Propionibacterium freudenreichii (total 1.5 × 107 CFU/mL), P2: P1 with added Bacillus subtilis and B. licheniformis (total 5.9 × 107 CFU/mL). The DFM were anaerobically incubated in rumen fluid and buffer with freeze-dried silage samples for 48 h. Total gas production (TGP: mL at Standard Temperature and Pressure/gram of organic matter), pH, organic matter degradability (dOM), CH4concentration (MC) and yield (MY), and volatile fatty acid (VFA) production and profiles were measured after fermentation. No significant differences between the laboratories were detected for any response variables. The dOM of MS (78.3%) was significantly less than GS (81.4%), regardless of the DFM added (P1 and P2). There were no significant differences between the effects of the DFM within the feed type. MS produced significantly more gas than GS after 48 h, but GS with DFM produced significantly more gas at 3 and 9 h and a similar gas volume at 12 h. Both DFM increased TGP significantly in GS at 48 h. There was no difference in total VFA production. However, GS with and without probiotics produced significantly more propionic acid and less butyric acid than MS with and without probiotics. Adding P2 numerically reduced the total methane yield by 4–6% in both MS and GS. The fermentation duration of 48 h, used to determine maximum potential dOM, may give misleading results. This study showed that it is possible to standardize the methodology to achieve reproducibility of IVGPT results. Furthermore, the results suggest that the P2 DFM may have the potential to reduce CH4 production without affecting organic matter degradation.
Biomethane Production from Sugarcane Vinasse in a Circular Economy: Developments and Innovations
Authors: de Carvalho Júlio Cesar , de Souza Vandenberghe Luciana Porto , Sydney Eduardo Bittencourt , Karp Susan Grace , Magalhães Antonio Irineudo , Martinez-Burgos Walter José , Medeiros Adriane Bianchi Pedroni , Thomaz-Soccol Vanete , Vieira Sabrina , Letti Luiz Alberto Junior , Rodrigues Cristine , Woiciechowski Adenise Lorenci , Soccol Carlos Ricardo
Published: April 2023

Sugarcane ethanol production generates about 360 billion liters of vinasse, a liquid effluent with an average chemical oxygen demand of 46,000 mg/L. Vinasse still contains about 11% of the original energy from sugarcane juice, but this chemical energy is diluted. This residue, usually discarded or applied in fertigation, is a suitable substrate for anaerobic digestion (AD). Although the technology is not yet widespread—only 3% of bioethanol plants used it in Brazil in the past, most discontinuing the process—the research continues. With a biomethane potential ranging from 215 to 324 L of methane produced by kilogram of organic matter in vinasse, AD could improve the energy output of sugarcane biorefineries. At the same time, the residual digestate could still be used as an agricultural amendment or for microalgal production for further stream valorization. This review presents the current technology for ethanol production from sugarcane and describes the state of the art in vinasse AD, including technological trends, through a recent patent evaluation. It also appraises the integration of vinasse AD in an ideal sugarcane biorefinery approach. It finally discusses bottlenecks and presents possible directions for technology development and widespread adoption of this simple yet powerful approach for bioresource recovery.
Improving the Physical and Mechanical Characteristics of Modified Aerated Concrete by Reinforcing with Plant Fibers
Authors: Beskopylny Alexey N. , Shcherban’ Evgenii M. , Stel’makh Sergey A. , Mailyan Levon R. , Meskhi Besarion , Evtushenko Alexandr , El’shaeva Diana , Chernil’nik Andrei
Published: April 2023

An urgent and promising direction in the development of building materials science is the improvement of the quality of non-autoclaved aerated concrete. In view of the obvious disadvantages of non-autoclaved aerated concrete compared to the autoclaved equivalent in terms of technology, it can be significantly improved because of a rationally selected composition and other factors of a recipe-technological nature. The goal of the study was to search for complex compositions and technological solutions aimed at identifying rational combinations of recipe-technological factors as simultaneous modifications of aerated concrete with various additives and dispersed the reinforcement of it with various environmentally friendly and cost-effective types of plant fibers. Fly ash (FA), instead of part of the cement, proved to be more effective than the GGBS additive. The compressive strength (CS), bending strength (BS), and coefficient of construction quality (CCQ) were higher by 4.5%, 3.8%, and 1.7%, respectively, while the density and thermal conductivity (TC) were lower by 0.7% and 3.6%, respectively, compared with aerated concrete modified with ground granulated blast-furnace slag (GGBS). The additional reinforcement of modified aerated concrete with coconut fiber (CF) and sisal fiber (SF) in an amount of 0.6% of the total mass of cement and modifier increases the CS to 15%, BS to 22% and CCQ to 16%. The SF was more effective than the CF. Aerated concrete modified with FA and reinforced with SF showed the highest efficiency. Compared to the control composition without modifiers or fibers, the increase in the CS was up to 40%, BS up to 47%, and CCQ up to 43%, while the decrease in density was up to 2.6%, and TC up to 15%.
Thermorheological Behavior of κ-Carrageenan Hydrogels Modified with Xanthan Gum
Authors: Avallone Pietro Renato , Russo Spena Simona , Acierno Stefano , Esposito Maria Giovanna , Sarrica Andrea , Delmonte Marco , Pasquino Rossana , Grizzuti Nino
Published: April 2023

Hydrocolloids are long-chain biopolymers that can form viscous solutions or gels when dissolved in water. They are employed as rheological modifiers in various manufacturing processes or finished products. Due to its unique gelation properties, animal gelatin is one of the most widely used hydrocolloids, finding applications in several fields such as food, pharmaceutical, and photographic. Nowadays, the challenge of finding valid alternatives to animal products has become a crucial issue, for both ethical and environmental reasons. The aim of this work, is to propose a green hydrocolloidal network, able to reproduce the gelation features of animal gelatin gels. κ-carrageenan gels may be an interesting alternative to gelatin, due to their attractive gelling features. We investigate the thermorheological behavior of κ-carrageenan aqueous solutions at various concentrations, focusing on gel features such as transition temperature and gel strength. To improve the viscoelastic response of such gels, we add a viscosity-enhancing hydrocolloid, i.e., xanthan gum. The results show that the gel strength increases exponentially with xanthan concentration, thus suggesting a synergistic interaction between the two networks. We also study the effect of sucrose on the thermal and mechanical properties of modified gels, finding a marked increase in transition temperatures and gel elasticity. In recent years, three-dimensional (3D) food printing has been extensively studied in the food industry, due to its many advantages, such as customized food design, personalized nutrition, simplified supply chain, and the expansion of available food materials. In view of this growing interest for additive manufacturing, we also study the printability of the complete formulation composed of κ-carrageenan, xanthan gum and sucrose.
Thermal Performance Evaluation of Plate-Type Heat Exchanger with Alumina–Titania Hybrid Suspensions
Authors: Bhattad Atul , Rao Boggarapu Nageswara , Atgur Vinay , Veza Ibham , Zamri Mohd Faiz Muaz Ahmad , Fattah Islam Md Rizwanul
Published: April 2023

This paper aims to develop models for the thermal conductivity and viscosity of hybrid nanofluids of aluminium oxide and titanium dioxide (Al2O3-TiO2). The study investigates the impact of fluid temperature (283 K–298 K) on the performance of a plate heat exchanger using Al2O3-TiO2 hybrid nanofluids with different particle volume ratios (0:5, 1:4, 2:3, 3:2, 4:1, and 5:0) prepared with a 0.1% concentration in deionised water. Experimental evaluations were conducted to assess the heat transfer rate, Nusselt number, heat transfer coefficient, Prandtl number, pressure drop, and performance index. Due to the lower thermal conductivity of TiO2 nanoparticles compared to Al2O3, a rise in the TiO2 ratio decreased the heat transfer coefficient, Nusselt number, and heat transfer rate. Inlet temperature was found to decrease pressure drop and performance index. The Al2O3 (5:0) nanofluid demonstrated the maximum enhancement of around 16.9%, 16.9%, 3.44%, and 3.41% for the heat transfer coefficient, Nusselt number, heat transfer rate, and performance index, respectively. Additionally, the TiO2 (0:5) hybrid nanofluid exhibited enhancements of 0.61% and 2.3% for pressure drop and Prandtl number, respectively. The developed hybrid nanofluids enhanced the performance of the heat exchanger when used as a cold fluid.
Reducing the Flocculation of Milk Tea Using Different Stabilizers to Regulate Tea Proteins
Authors: Song Yuqi , Wang Xiaosen , Luo Haixi , Wang Mingyan , Chen Jian
Published: April 2023

The regulation of flocs derived from polyphenol–protein formation in milk tea has not been fully explored. In this study, the flocculation of milk tea was regulated by adding 10 kinds of stabilizers with different characteristics. The stability coefficient and centrifugal precipitation rate were used as indexes. The optimal concentration ratio of the complex stabilizer was identified using the response surface methodology (RSM), being 0.04% for Arabic gum, 0.02% for β-cyclodextrin and 0.03% for Agar. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) were used to analyze the characteristics of different stabilizers in milk tea, and our findings were as follows: (1) The relative strength of the peaks in different stable systems was different. The absorption peaks were mainly near the wave numbers 3376 cm−1, 2928 cm−1, 1655 cm−1, 1542 cm−1, 1408 cm−1, 1047 cm−1 and 925 cm−1. (2) The milk tea system was an amorphous structure. The diffraction peak of the composite system was observed to be about 20°. The crystallinity of the milk tea in the compound group was 33.16%, which was higher than that of the blank group (9.67%). (3) The compound stabilizer reduced flocculation, and the stabilizing agents improved the surface order of milk tea. These results indicate that the combination of polysaccharide stabilizers (Arabic gum and agar) and oligosaccharide stabilizers (β-CD) in certain proportions can regulate the flocculation of milk tea and improve its stability. The potential research avenues involving polyphenol–protein complex instability systems and their applications in food development are expanded by this work.
Polyphenols in Jabuticaba (Plinia spp.) Peel Flours: Extraction and Comparative Evaluation of FTIR and HPLC for Quantification of Individual Compounds
Authors: Resende Laís , Oliveira Leandro , Franca Adriana
Published: April 2023

Jabuticabas are wild fruits native to Brazil, and their peels, the main residue from jabuticaba processing, contain significant amounts of bioactive compounds, which are mostly phenolics. Conventional methods based on the estimation of total extractable phenolics (TEP—Folin–Ciocalteau) or total monomeric anthocyanins (TMA) have limitations and may not reflect the actual antioxidant potential of these peels. Analytical methods, such as high-performance liquid chromatography (HPLC), are more appropriate for the quantification of specific phenolics, and can be used as a reference for the construction of mathematical models in order to predict the amount of compounds using simple spectroscopic analysis, such as Fourier Transform Infrared Spectroscopy (FTIR). Therefore, the objectives of this study were (i) to evaluate the composition of specific polyphenols in flours prepared from jabuticaba peels and verify their correlation with TEP and TMA results from a previous study, and (ii) to employ FTIR coupled with chemometrics to predict the concentrations of these polyphenols in jabuticaba peel flours (JPFs) using HPLC as a reference method. Cyanidin-3-glucoside (C3G), ellagic acid (EA) and delphinidin-3-glucoside (D3G) were the main polyphenols found in the samples. The C3G contents ranged from 352.33 mg/100 g (S10) to 1008.73 mg/100 g (S22), with a strong correlation to TMA (r = 0.97; p = 0.00) and a moderate correlation to TEP (r = 0.45; p = 0.02). EA contents ranged from 163.65 mg/100 g (S23) to 334.69 mg/100 g (S11), with a moderate to strong correlation to TEP (r = 0.69; p = 0.00). The D3G values ranged from 94.99 mg/100 g (S10) to 203.36 mg/100 g (S5), with strong correlations to TMA (r = 0.91; p = 0.00) and C3G levels (r = 0.92; p = 0.00). The developed partial least squares-PLS models based on FTIR data provided satisfactory predictions of C3G and EA levels, reasonably matching those of HPLC.
Plant-Based Alternatives to Cheese Formulated Using Blends of Zein and Chickpea Protein Ingredients
Authors: Grasso Nadia , Bot Francesca , Roos Yrjo H. , Crowley Shane V. , Arendt Elke K. , O’Mahony James A.
Published: April 2023

In this study, zein protein isolate (ZPI) and chickpea protein concentrate (CPC) ingredients were used to formulate five plant-based cheese alternatives. Ingredient ratios based on protein contributions of 0:100, 25:75, 50:50, 75:25 and 100:0 from ZPI and CPC, respectively, were used. Formulations were developed at pH ~4.5, with a moisture target of 59%. Shea butter was used to target 15% fat, while tapioca starch was added to target the same carbohydrate content for all samples. Microstructural analysis showed differences among samples, with samples containing ZPI displaying a protein-rich layer surrounding the fat globules. Schreiber meltability and dynamic low amplitude oscillatory shear rheological analyses showed that increasing the proportion of ZPI was associated with increasing meltability and greater ability to flow at high temperatures. In addition, the sample containing only CPC showed the highest adhesiveness, springiness and cohesiveness values from the texture profile analysis, while the sample containing only ZPI exhibited the highest hardness. Furthermore, stretchability increased with increasing ZPI proportions. This work will help understanding of the role and potential of promising plant-protein-ingredient blends in formulating plant-based alternatives to cheese with desirable functional properties.
Occurrence and Characterization of Microplastics in Commercial Mussels (Mytilus galloprovincialis) from Apulia Region (Italy)
Authors: Dambrosio Angela , Cometa Stefania , Capuozzo Flavia , Ceci Edmondo , Derosa Michele , Quaglia Nicoletta Cristiana
Published: April 2023

Microplastics are a ubiquitous pollutant whose spreading is a growing concern worldwide. They can pose a threat to food safety and consumer health as they are ingested through various foods. Bivalves are considered the most contaminated, as they filter large amounts of seawater and enter consumers’ diet ingested whole. The aim of this study was to detect, quantify, identify and classify microplastics in mussels (Mytilus galloprovincialis) marketed in fishery stores in Bari and its surroundings (Apulia, Italy). A total of 5077 particles were isolated from our samples, with an average value of 1.59 ± 0.95 MPs/g and 6.51 ± 4.32 MPs/individual. Blue fragments, sized 10–500 µm, were the prevalent findings; most of them belonged to Polyamide (PA) polymers. The results of this study help to show that mussels represent a source of microplastics for consumers and a direct risk to their health, even considering that they may contain many chemical compounds and microorganisms that may or may not be pathogenic to humans. Further research is needed to assess the role of commercialization in bivalve molluscs contamination.
Microencapsulated and Ready-to-Eat Beetroot Soup: A Stable and Attractive Formulation Enriched in Nitrate, Betalains and Minerals
Authors: Trindade Lucileno Rodrigues da , Baião Diego dos Santos , da Silva Davi Vieira Teixeira , Almeida Cristine Couto , Pauli Fernanda Petzold , Ferreira Vitor Francisco , Conte-Junior Carlos Adam , Paschoalin Vania Margaret Flosi
Published: April 2023

Beetroot is a tuber rich in antioxidant compounds, i.e., betanin and saponins, and is one of the main sources of dietary nitrate. The aim of the present study was to microencapsulate a ready-to-eat beetroot soup by lyophilization using different encapsulating agents, which supply the required amount of bioactive nutrients. Particle size distributions ranged from 7.94 ± 1.74 to 245.66 ± 2.31 µm for beetroot soup in starch and from 30.56 ± 1.66 to 636.34 ± 2.04 µm in maltodextrin. Microparticle yields of powdered beetroot soup in starch varied from 77.68% to 88.91%, and in maltodextrin from 75.01% to 80.25%. The NO3− and total betalain contents at a 1:2 ratio were 10.46 ± 0.22 mmol·100 g−1 fresh weight basis and 219.7 ± 4.92 mg·g−1 in starch powdered beetroot soup and 8.43 ± 0.09 mmol·100 g−1 fresh weight basis and 223.9 ± 4.21 mg·g−1 in maltodextrin powdered beetroot soup. Six distinct minerals were identified and quantified in beetroot soups, namely Na, K, Mg, Mn, Zn and P. Beetroot soup microencapsulated in starch or maltodextrin complied with microbiological quality guidelines for consumption, with good acceptance and purchase intention throughout 90 days of storage. Microencapsulated beetroot soup may, thus, comprise a novel attractive strategy to offer high contents of bioaccessible dietary nitrate and antioxidant compounds that may aid in the improvement of vascular-protective effects.
A Machine Learning Method to Identify Umami Peptide Sequences by Using Multiplicative LSTM Embedded Features
Authors: Jiang Jici , Li Jiayu , Li Junxian , Pei Hongdi , Li Mingxin , Zou Quan , Lv Zhibin
Published: April 2023

Umami peptides enhance the umami taste of food and have good food processing properties, nutritional value, and numerous potential applications. Wet testing for the identification of umami peptides is a time-consuming and expensive process. Here, we report the iUmami-DRLF that uses a logistic regression (LR) method solely based on the deep learning pre-trained neural network feature extraction method, unified representation (UniRep based on multiplicative LSTM), for feature extraction from the peptide sequences. The findings demonstrate that deep learning representation learning significantly enhanced the capability of models in identifying umami peptides and predictive precision solely based on peptide sequence information. The newly validated taste sequences were also used to test the iUmami-DRLF and other predictors, and the result indicates that the iUmami-DRLF has better robustness and accuracy and remains valid at higher probability thresholds. The iUmami-DRLF method can aid further studies on enhancing the umami flavor of food for satisfying the need for an umami-flavored diet.
Production of a Series of Long-Chain Isomaltooligosaccharides from Maltose by Bacillus subtilis AP-1 and Associated Prebiotic Properties
Authors: Tiangpook Suratsawadee , Nhim Sreyneang , Prangthip Pattaneeya , Pason Patthra , Tachaapaikoon Chakrit , Ratanakhanokchai Khanok , Waeonukul Rattiya
Published: April 2023

Bacillus subtilis strain AP-1, which produces α-glucosidase with transglucosidase activity, was used to produce a series of long-chain isomaltooligosaccharides (IMOs) with degree of polymerization (DP) ranging from 2 to 14 by direct fermentation of maltose. A total IMOs yield of 36.33 g/L without contabacillusmination from glucose and maltose was achieved at 36 h of cultivation using 50 g/L of maltose, with a yield of 72.7%. IMOs were purified by size exclusion chromatography with a Superdex 30 Increase column. The molecular mass and DP of IMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Subsequently, linkages in produced oligosaccharides were verified by enzymatic hydrolysis with α-amylase and oligo-α-1,6-glucosidase. These IMOs showed prebiotic properties, namely tolerance to acidic conditions and digestive enzymes of the gastrointestinal tract, stimulation of probiotic bacteria growth to produce short-chain fatty acids and no stimulating effect on pathogenic bacteria growth. Moreover, these IMOs were not toxic to mammalian cells at up to 5 mg/mL, indicating their biocompatibility. Therefore, this research demonstrated a simple and economical method for producing IMOs with DP2–14 without additional operations; moreover, the excellent prebiotic properties of the IMOs offer great prospects for their application in functional foods.
Quality Improvement of Garlic Paste by Whey Protein Isolate Combined with High Hydrostatic Pressure Treatment
Authors: Zang Baoyuan , Qiu Zhichang , Zheng Zhenjia , Zhang Bin , Qiao Xuguang
Published: April 2023

Garlic, one of the most popular spices and medical herbs, has a unique pungent flavor and taste. Conventional homogenization and thermal treatment commonly lead to flavor and color deterioration in garlic paste, because allicin is highly susceptible to degradation and reaction. The present study was to investigate the effects of whey protein isolate (WPI) and different levels of high hydrostatic pressure (HHP, 200, 300, 400, 500, and 600 MPa) on the quality of garlic paste. Results showed that the addition of WPI in the homogenization of garlic significantly prevented green discoloration. Furthermore, WPI plus HHP under 500 MPa could better protect the color of garlic paste. Higher pressure (600 MPa) led to WPI aggregation, resulting in higher green color chroma of garlic paste. GC-MS results revealed that the application of WPI and HHP in garlic paste increased the relative level of pungent flavor compounds and decreased those of unpleasant odor compounds. The correlation analysis results revealed that WPI efficiently prevented garlic green discoloration, which is attributed to the thiol group in WPI exchanging the sulfonyl groups in allicin. In consideration of the microbial load, flavor and color quality of garlic paste, the optimal processing conditions were found at 500 MPa for 5 min with 2% WPI addition, extending shelf life to 25 days.
Targeted HPLC-UV Polyphenolic Profiling to Detect and Quantify Adulterated Tea Samples by Chemometrics
Authors: Romers Thom , Saurina Javier , Sentellas Sònia , Núñez Oscar
Published: April 2023

Tea can be found among the most widely consumed beverages, but it is also highly susceptible to fraudulent practices of adulteration with other plants such as chicory to obtain an illicit economic gain. Simple, feasible and cheap analytical methods to assess tea authentication are therefore required. In the present contribution, a targeted HPLC-UV method for polyphenolic profiling, monitoring 17 polyphenolic and phenolic acids typically described in tea, was proposed to classify and authenticate tea samples versus chicory. For that purpose, the obtained HPLC-UV polyphenolic profiles (based on the peak areas at three different acquisition wavelengths) were employed as sample chemical descriptors for principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) studies. Overall, PLS-DA demonstrated good sample grouping and discrimination of chicory against any tea variety, but also among the five different tea varieties under study, with classification errors below 8% and 10.5% for calibration and cross-validation, respectively. In addition, the potential use of polyphenolic profiles as chemical descriptors to detect and quantify frauds was evaluated by studying the adulteration of each tea variety with chicory, as well as the adulteration of red tea extracts with oolong tea extracts. Excellent results were obtained in all cases, with calibration, cross-validation, and prediction errors below 2.0%, 4.2%, and 3.9%, respectively, when using chicory as an adulterant, clearly improving on previously reported results when using non-targeted HPLC-UV fingerprinting methodologies.
Emulsion-Based Delivery Systems to Enhance the Functionality of Bioactive Compounds: Towards the Use of Ingredients from Natural, Sustainable Sources
Authors: Teixé-Roig Júlia , Oms-Oliu Gemma , Odriozola-Serrano Isabel , Martín-Belloso Olga
Published: April 2023

In recent years, the trend in the population towards consuming more natural and sustainable foods has increased significantly. This claim has led to the search for new sources of bioactive compounds and extraction methods that have less impact on the environment. Moreover, the formulation of systems to protect these compounds is also focusing on the use of ingredients of natural origin. This article reviews novel, natural alternative sources of bioactive compounds with a positive impact on sustainability. In addition, it also contains information on the most recent studies based on the use of natural (especially from plants) emulsifiers in the design of emulsion-based delivery systems to protect bioactive compounds. The properties of these natural-based emulsion-delivery systems, as well as their functionality, including in vitro and in vivo studies, are also discussed. This review provides relevant information on the latest advances in the development of emulsion delivery systems based on ingredients from sustainable natural sources.
A Machine Learning Method for the Quality Detection of Base Liquor and Commercial Liquor Using Multidimensional Signals from an Electronic Nose
Authors: Li Bingyang , Gu Yu
Published: April 2023

Chinese liquor is a world-famous beverage with a long history. Base liquor, a product of liquor brewing, significantly affects the flavor and quality of commercial liquor. In this study, a machine learning method consisting of a deep residual network (ResNet)18 backbone with a light gradient boosting machine (LightGBM) classifier (ResNet-GBM) is proposed for the quality identification of base liquor and commercial liquor using multidimensional signals from an electronic nose (E-Nose). Ablation experiments are conducted to analyze the contribution of the framework’s components. Five evaluation metrics (accuracy, sensitivity, precision, F1 score, and kappa score) are used to verify the performance of the proposed method, and six other frameworks (support vector machine (SVM), random forest (RF), k-nearest neighbor (KNN), extreme gradient boosting (XGBoost), multidimensional scaling-support vector machine (MDS-SVM), and back-propagation neural network (BPNN)) on three datasets (base liquor, commercial liquor, and mixed base and commercial liquor datasets). The experimental results demonstrate that the proposed ResNet-GBM model achieves the best performance for identifying base liquor and commercial liquors with different qualities. The proposed framework has the highest F1 score for the identification of commercial liquor in the mixed dataset due to the contribution of similar microconstituents from the base liquor. The proposed method can be used for the quality control of Chinese liquor and promotes the practical application of E-nose devices.
Advances in Nanogels for Topical Drug Delivery in Ocular Diseases
Authors: Wu Yongkang , Tao Qing , Xie Jing , Lu Lili , Xie Xiuli , Zhang Yang , Jin Yong
Published: April 2023

Nanotechnology has accelerated the development of the pharmaceutical and medical technology fields, and nanogels for ocular applications have proven to be a promising therapeutic strategy. Traditional ocular preparations are restricted by the anatomical and physiological barriers of the eye, resulting in a short retention time and low drug bioavailability, which is a significant challenge for physicians, patients, and pharmacists. Nanogels, however, have the ability to encapsulate drugs within three-dimensional crosslinked polymeric networks and, through specific structural designs and distinct methods of preparation, achieve the controlled and sustained delivery of loaded drugs, increasing patient compliance and therapeutic efficiency. In addition, nanogels have higher drug-loading capacity and biocompatibility than other nanocarriers. In this review, the main focus is on the applications of nanogels for ocular diseases, whose preparations and stimuli-responsive behaviors are briefly described. The current comprehension of topical drug delivery will be improved by focusing on the advances of nanogels in typical ocular diseases, including glaucoma, cataracts, dry eye syndrome, and bacterial keratitis, as well as related drug-loaded contact lenses and natural active substances.
Temperature- and pH-Responsive Super-Absorbent Hydrogel Based on Grafted Cellulose and Capable of Heavy Metal Removal from Aqueous Solutions
Authors: Tohamy Hebat-Allah S. , El-Sakhawy Mohamed , Strachota Beata , Strachota Adam , Pavlova Ewa , Mares Barbosa Silvia , Kamel Samir
Published: April 2023

In this work, we prepared highly swelling, stimuli-responsive hydrogels capable of the highly efficient adsorption of inorganic pollutants. The hydrogels were based on hydroxypropyl methyl cellulose (HPMC) grafted with acrylamide (AM) and 3-sulfopropyl acrylate (SPA) and were synthesized via the growth (radical polymerization) of the grafted copolymer chains on HPMC, which was activated by radical oxidation. These grafted structures were crosslinked to an infinite network by a small amount of di-vinyl comonomer. HPMC was chosen as a cheap hydrophilic and naturally sourced polymer backbone, while AM and SPA were employed to preferentially bond coordinating and cationic inorganic pollutants, respectively. All the gels displayed a pronounced elastic character, as well as considerably high values of stress at break (several hundred %). The gel with the highest fraction of the ionic comonomer SPA (with an AM/SPA ratio = 0.5) displayed the highest equilibrium swelling ratio (12,100%), the highest volume response to temperature and pH, and the fastest swelling kinetics, but also the lowest modulus. The other gels (with AM/SPA = 1 and 2) displayed several times higher moduli but more modest pH responses and only very modest temperature sensitivity. Cr(VI) adsorption tests indicated that the prepared hydrogels removed this species from water very efficiently: between 90 and 96% in one step. The hydrogels with AM/SPA ratios of 0.5 and 1 appeared to be promising regenerable (via pH) materials for repeated Cr(VI) adsorption.
Highly Efficient Capture of Heavy Metal Ions on Amine-Functionalized Porous Polymer Gels
Authors: He Xue , Xia Jumu , He Jieli , Qi Kezhen , Peng Anzhong , Liu Yong
Published: April 2023

Porous polymer gels (PPGs) are characterized by inherent porosity, a predictable structure, and tunable functionality, which makes them promising for the heavy metal ion trap in environmental remediation. However, their real-world application is obstructed by the balance between performance and economy in material preparation. Development of an efficient and cost-effective approach to produce PPGs with task-specific functionality remains a significant challenge. Here, a two-step strategy to fabricate amine-enriched PPGs, NUT-21-TETA (NUT means Nanjing Tech University, TETA indicates triethylenetetramine), is reported for the first time. The NUT-21-TETA was synthesized through a simple nucleophilic substitution using two readily available and low-cost monomers, mesitylene and α, α′-dichloro-p-xylene, followed by the successful post-synthetic amine functionalization. The obtained NUT-21-TETA demonstrates an extremely high Pb2+ capacity from aqueous solution. The maximum Pb2+ capacity, qm, assessed by the Langmuir model was as high as 1211 mg/g, which is much higher than most benchmark adsorbents including ZIF-8 (1120 mg/g), FGO (842 mg/g), 732-CR resin (397 mg/g), Zeolite 13X (541 mg/g), and AC (58 mg/g). The NUT-21-TETA can be regenerated easily and recycled five times without a noticeable decrease of adsorption capacity. The excellent Pb2+ uptake and perfect reusability, in combination with a low synthesis cost, gives the NUT-21-TETA a strong potential for heavy metal ion removal.
Characterization of a Graphene Oxide-Reinforced Whey Hydrogel as an Eco-Friendly Absorbent for Food Packaging
Authors: Purcea Lopes Pompilia Mioara , Moldovan Dumitrita , Fechete Radu , Mare Liviu , Barbu-Tudoran Lucian , Sechel Niculina , Popescu Violeta
Published: April 2023

This study presents a structural analysis of a whey and gelatin-based hydrogel reinforced with graphene oxide (GO) by ultraviolet and visible (UV-VIS) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD). The results revealed barrier properties in the UV range for the reference sample (containing no graphene oxide) and the samples with minimal GO content of 0.66×10−3% and 3.33×10−3%, respectively, in the UV-VIS and near-IR range; for the samples with higher GO content, this was 6.67×10−3% and 33.33×10−3% as an effect of the introduction of GO into the hydrogel composite. The changes in the position of diffraction angles 2θ from the X-ray diffraction patterns of GO-reinforced hydrogels indicated a decrease in the distances between the turns of the protein helix structure due to the GO cross-linking effect. Transmission electron spectroscopy (TEM) was used for GO, whilst scanning electron microscopy (SEM) was used for the composite characterization. A novel technique for investigating the swelling rate was presented by performing electrical conductivity measurements, the results of which led to the identification of a potential hydrogel with sensor properties.
Exploration of Reactive Black 5 Dye Desorption from Composite Hydrogel Beads—Adsorbent Reusability, Kinetic and Equilibrium Isotherms
Authors: Grigoraș Cristina-Gabriela , Simion Andrei-Ionuț , Favier Lidia
Published: April 2023

A low-cost adsorbent was prepared by using cherry stones powder and chitosan and used to retain Reactive Black 5 dye from aqueous solution. Then, the spent material was submitted to a regeneration process. Five different eluents (water, sodium hydroxide, hydrochloric acid, sodium chloride and ethanol) were tested. Among them, sodium hydroxide was selected for an advanced investigation. Values of three working conditions, namely the eluent volume, its concentration and the desorption temperature, were optimized by Response Surface Methodology-Box–Behnken Design. In the established settings (NaOH volume: 30 mL, NaOH concentration: 1.5 M, working temperature: 40 °C), three successive cycles of adsorption/desorption were conducted. The analysis performed by Scanning Electron Microscopy and by Fourier Transform Infrared Spectroscopy revealed the evolution of the adsorbent throughout the dye elution from the material. Pseudo-second-order kinetic model and Freundlich equilibrium isotherm were able to accurately describe the desorption process. Based on the acquired results, our outcomes sustain the suitability of the synthesized material as dye adsorbent and the possibility of efficaciously recycling and reusing it.
Assessing the Predictive Power of Frailty and Life-Space Mobility on Patient-Reported Outcomes of Disability in Older Adults with Low Back Pain
Authors: Thonprasertvat Benyapa , Roopsawang Inthira , Aree-Ue Suparb
Published: April 2023

Background: Frailty and decreased life-space mobility are known as risk factors to develop physical limitations leading to disability in older adults with low back pain (LBP). This cross sectional study aimed to investigate the prevalence and predictive power of frailty and life-space mobility on patient-reported outcomes of disability in older adults with LBP. Methods: The sample comprised 165 older adults with LBP who visited two tertiary care hospitals between December 2021 and February 2022. The participants responded to structured standard questionnaires. Data were analyzed using descriptive statistics and robust logistic regression. Results: More than two-thirds of participants were classified as non-frail (26.67%) or pre-frail (66.67%). Mobility restrictions and minimal to severe disability were identified. Controlling other variables, frailty (OR = 1.74, 95% CI: 1.14–2.64) and restricted life-space mobility (OR = 0.42, 95% CI: 0.26–0.67) were significantly associated with disability. Integrating frailty with life-space mobility evaluations demonstrated the highest predictive power for disability-related LBP (AUC = 0.89, 95% CI: 0.84–0.93). Conclusion: Frailty and restricted life-space mobility significantly predicted disability in older adults with LBP. Healthcare professionals should recognize the critical importance of integrating patient-reported outcomes with screening for frailty and life-space mobility limitation to optimize care or tract symptom progression.
Knowledge about Palliative Care and Attitudes toward Care of the Dying among Primary Care Nurses in Spain
Authors: Mengual Tamara Escoto , Chover-Sierra Elena , Ballestar-Tarín María Luisa , Saus-Ortega Carles , Gea-Caballero Vicente , Colomer-Pérez Natura , Martínez-Sabater Antonio
Published: April 2023

Objective: To analyze the knowledge in palliative care and the attitudes toward caring for the dying of nurses who carry out their professional activity in primary care in Spain. Design: A cross-sectional descriptive observational study was carried out among Spanish primary care centers. Participants: A total of 244 nurses who had completed their primary care work and agreed to participate in this study were included. Main measurements: The level of knowledge in palliative care was analyzed using the PCQN-SV scale, and attitudes toward care of the dying were measured with the FATCOD-S scale, both of which are instruments that are validated in Spain. Results: Regarding the level of knowledge in palliative care, at a global level, the results revealed that 60% of the answers in the PCQN-SV were correct, with different results for each of the three subscales that compose it. When analyzing the attitudes of primary care nurses toward the care of the dying, an average of 132.21 out of 150 was obtained, representing a positive attitude. On the other hand, when analyzing these results in terms of knowledge and attitudes according to the population’s characteristics, we see that participants with both experience and training in palliative care present a better level of knowledge and a higher score regarding their attitudes toward care of the dying. However, the differences are only significant regarding the level of knowledge in palliative care. When analyzing the data from the two scales together, it is noteworthy that the participants with the most positive attitudes (highest scores on the FATCOD-S) also have the highest percentages of correct answers on the PCQN-SV.
Validity of On-Line Supervised Fitness Tests in People with Low Back Pain
Authors: Lavín-Pérez Ana Myriam , León-Llamas Juan Luis , Salas Costilla Francisco José , Collado-Mateo Daniel , López de las Heras Raúl , Gasque Celma Pablo , Villafaina Santos
Published: April 2023

This study aimed to investigate the concurrent validity between online evaluations (OEs) and face-to-face evaluations (IPEs) of a Senior Fitness Test and two balance tests in people with low back pain (LBP). Forty participants of 58.48 (9.87) years were included. The 30 s chair stand-up, arm curl, 2 min step, chair-sit and reach, back scratch, 8 foot up-and-go, sharpened Romberg, and one-legged stance tests were administrated using both OE and IPE methods. The results indicated no significant differences (p > 0.05) between the two methods except in the 8-foot up-and-go test (p = 0.007). Considering the ICC values and Bland-Altman plots, excellent agreement was found for the chair-sit and reach test, moderate agreement for the arm-curl and 8-foot up-and-go tests, and good agreement for the other tests. Strong correlations (p < 0.001) were observed in all variables except for the arm-curl and 8-foot up-and-go tests, where moderate correlations were found (p < 0.05). These results support the validity of OEs and IPEs in all tests, except for the arm-curl and 8-foot up-and-go tests, where lower ICC values and moderate correlations were found. However, it is important to consider the range of fluctuation of the ICC and the significant values obtained through correlations.
Growth, Anatomical, and Biochemical Responses of the Space Farming Candidate Brassica rapa L. Microgreens to Low-LET Ionizing Radiation
Authors: De Francesco Sara , Amitrano Chiara , Vitale Ermenegilda , Costanzo Giulia , Pugliese Mariagabriella , Arrichiello Cecilia , Ametrano Gianluca , Muto Paolo , Arena Carmen , De Micco Veronica
Published: April 2023

An adequate and balanced diet is fundamental in preserving the health of astronauts from several space-induced diseases. Therefore, the integration of a diet with fresh food, rich in bioactive compounds such as microgreens produced directly onboard, may be useful in space for human nutrition. However, ionizing radiation (IR) in space represents a significant hindrance for organisms, with potential critical outcomes on plant morpho-anatomical, eco-physiological, and biochemical aspects, depending on the plant and IR features (e.g., species, developmental stage, IR dose, and type). In this study, we analyzed the effect of different doses of X-rays (0-control, 0.3, 1, 10, 20, and 30 Gy) on the morpho-anatomical and nutritional traits of microgreens of Brassica rapa L., irradiated at the stage of germinated seeds. After the irradiation, microgreens were cultivated in controlled conditions. At harvest, the morpho-biometric traits were analyzed, along with the leaf functional anatomical traits and the phytochemical content of the aboveground biomass. The results showed that X-ray exposure does not induce detrimental effects on growth, while it stimulates the production of antioxidants, improving plant defense and nutritional value. The overall results support the idea of using this species in space as a supplemental functional food.
Associations between HIV Status, SARS-CoV-2 Infection, Increase in Use of Psychoactive Substances and Oral Ulcers among People Who Used Psychoactive Substances during the First Wave of the COVID-19 Pandemic
Authors: Folayan Morenike Oluwatoyin , Zuñiga Roberto Ariel Abeldaño , Virtanen Jorma I. , Ezechi Oliver C. , Aly Nourhan M. , Lusher Joanne , Nguyen Annie L. , El Tantawi Maha
Published: April 2023

The aim of this study was to assess the associations between HIV status, SARS-CoV-2 infection, increase in use of psychoactive substances and oral ulcers among people who use psychoactive substances. This was a secondary analysis of the data of 1087 people who used psychoactive substances collected during the first wave of the COVID-19 pandemic. The data extracted were confounding (age, sex, the highest level of education attained, employment status, emotional distress status), dependent (oral ulcers) and independent (SARS-CoV-2 infection, increase in alcohol consumption, smoking and use of other psychoactive substances, living with HIV) variables. A multivariate logistic regression model was constructed to determine the associations between the dependent and independent variables after adjusting for the confounding variables. Participants who had a history of SARS-CoV-2 infection (AOR:10.37) and people living with HIV (AOR:1.91) had higher odds of reporting oral ulcers. The finding suggests that people who used psychoactive substances, had COVID-19 and lived with HIV were at increased risk for oral ulcers during the first wave of the COVID-19 pandemic. Increased use of psychoactive substances was not associated with a significant increase in the risk for oral ulcers. Further research is needed to better understand the reasons for these findings.
Influence of Smoking on Periodontal and Implant Therapy: A Narrative Review
Authors: Madi Marwa , Smith Steph , Alshehri Sami , Zakaria Osama , Almas Khalid
Published: April 2023

Background: smoking is considered the most modifiable risk factor for periodontal disease. Objective: the aim of this narrative review is to emphasize the effect of smoking on periodontal and implant therapy. Methods: The authors reviewed the literature reporting the clinical outcomes of smoking on periodontal surgical and nonsurgical treatment. The impact of smoking on implant therapy and sinus lifting procedures were also reviewed. Results: Periodontal and implant therapy outcomes are adversely affected by smoking. Smokers respond less favorably to periodontal therapy and periodontal flap procedures as compared to nonsmokers. Clinical outcomes for smokers are 50–75% worse than for nonsmokers. Studies reveal that smokers experience a significantly lower reduction in pocket depth compared to nonsmokers as well as less bone growth after treating infra-bony defects with guided tissue regeneration. The relative risk of implant failure is significantly higher in patients who smoke 20 cigarettes or more per day compared to nonsmokers. Additionally, smoking has also been shown to increase postoperative wound dehiscence and infection rates following sinus floor elevation. Longitudinal studies on smoke cessation have shown a reduction in bone loss and probing depths for periodontitis patients after cessation compared to those who smoke. Conclusion: Smoking cessation can reduce probing depths and improve clinical attachment after nonsurgical periodontal therapy. There is insufficient evidence regarding the effect of smoking on peri-implantitis, as well as the loss of implants in the long-term.