bluuebunny/crossref_metadata_2025 huggingface dataset:
Intercropping Winter Lupin and Triticale Increases Weed Suppression and Total Yield
Authors: Carton Nicolas , Naudin Christophe , Piva Guillaume , Corre-Hellou Guénaëlle
Published: August 2020

Lupin (Lupinus sp.) produces protein-rich grains, but its adoption in cropping systems suffers from both its low competitive ability against weeds and its high yield variability. Compared with legume sole cropping, grain legume–cereal intercropping benefits include better weed suppression and higher yield and yield stability. However, the potential of enhancing crop competitive ability against weeds in additive winter grain legume–cereal intercrops is not well-known, and this potential in long crop cycles is even less studied. We studied how intercropping with a triticale (×Triticosecale) alters weed biomass and productivity of winter white lupin (Lupinus albus L.). The experimental setup consisted of eleven sites during a two-year period in western France. In each site-year, winter white lupin sole cropping was compared to winter white lupin-triticale intercropping in an additive sowing design. We found that intercropping reduced weed biomass at lupin flowering by an average of 63%. The rapid growth and high soil N acquisition of triticale compensated for the low competitive ability of lupin against weeds until lupin flowering. Competition from triticale in the intercrop reduced lupin grain yield (−34%), but intercropping produced a higher total grain yield (+37%) than did lupin sole cropping while maintaining the total protein grain yield.
Pig Organ Lesions Recorded in Different Abattoirs: A Statistical Approach to Assess the Comparability of Prevalence
Authors: Horst Ariane , Gertz Marvin , Hasler Mario , Krieter Joachim
Published: August 2020

Documented lesions of slaughtered pigs provide a high-density data-pool that could be valuable for the purpose of animal health monitoring and breeding. However, data quality and structure hamper the application of statistical methods. The present study provides an approach that enables statistical analysis and evaluates the comparability of lesion prevalence among abattoirs. The German Quality and Safety database provided data of recorded lung, pleura, liver, and heart lesions. Filter criteria were used to improve the data structure. Data of n = 8,004,769 animals, recorded in nine abattoirs over a period of 18 months, were analyzed. Lesion prevalences were successfully modeled by applying a generalized linear mixed model. To examine prevalence differences, the coefficient of variation (CV) on a six-monthly basis was calculated, and a grand mean test (GMT) of significance was applied. High variations in estimated prevalence occurred on abattoir, six-monthly and organ basis. The highest variation occurred in the lung (CV = 64.7%), whereas liver lesions showed the lowest variation (CV = 21.8%). The GMT enabled the visualization of these variations between abattoirs, organs and over time. Concerning the assessment of the comparability of prevalences, it provides a promising tool to monitor changes in lesion examination and to address divergent abattoirs.
Industrial Archaeology Applied to the Study of an Ancient Harvesting Machine: Three-Dimensional Modelling and Virtual Reconstruction
Authors: Rojas-Sola José Ignacio , del Río-Cidoncha Gloria , Coronil-García Ángel
Published: August 2020

This article shows the three-dimensional (3D) modelling and virtual reconstruction of an ancient harvesting machine developed at the beginning of the 19th century. SolidWorks software is employed to obtain the 3D model of this historical invention and its geometric documentation. The original material for the research is available on a farm located in the province of Cádiz (Spain). Thanks to the three-dimensional modelling performed, both its operation and the final assembly of this invention can be explained in detail in a coherent way. Having carried out the functional analysis, it can be verified that the machine combines well-performed chain-sprocket transmissions, which, together with complex gearboxes with parallel and bevel gears, make this reaper a very reliable machine. Furthermore, the inclusion of elements such as gimbal joints on shafts with possible misalignments, and clutches to adapt the operation of the machine to the needs of the operator, makes it highly versatile and functional without over-exerting the mechanics. From a technical point of view, the complex transmission systems, the perfect synchronization achieved between all its parts, and the combination of continuous oscillatory movements, such as that of sheaf compactors with intermittent movements as complex as that of the knotting system, are all worthy of note, and reveal the great work of engineering involved in this historical invention.
Development of A Nested-MultiLocus Sequence Typing Approach for A Highly Sensitive and Specific Identification of Xylella fastidiosa Subspecies Directly from Plant Samples
Authors: Cesbron Sophie , Dupas Enora , Beaurepère Quentin , Briand Martial , Montes-Borrego Miguel , Velasco-Amo Maria del Pilar , Landa Blanca B. , Jacques Marie-Agnès
Published: July 2020

Identification of sequence types (ST) of Xylella fastidiosa based on direct MultiLocus Sequence Typing (MLST) of plant DNA samples is partly efficient. In order to improve the sensitivity of X. fastidiosa identification, we developed a direct nested-MLST assay on plant extracted DNA. This method was performed based on a largely used scheme targeting seven housekeeping gene (HKG) loci (cysG, gltT, holC, leuA, malF, nuoL, petC). Samples analyzed included 49 plant species and two insect species (Philaenus spumarius, Neophilaenus campestris) that were collected in 2017 (106 plant samples in France), in 2018 (162 plant samples in France, 40 plant samples and 26 insect samples in Spain), and in 2019 (30 plant samples in Spain). With the nested approach, a significant higher number of samples were amplified. The threshold was improved by 100 to 1000 times compared to conventional PCR. Using nested-MLST assay, plants that were not yet considered hosts tested positive and revealed novel alleles in France, whereas for Spanish samples it was possible to assign the subspecies or ST to samples considered as new hosts in Europe. Direct typing by nested-MLST from plant material has an increased sensitivity and may be useful for epidemiological purposes.
Pharmacokinetics of a Long-Acting Formulation of Oxytetracycline in Freshwater Crocodiles (Crocodylus siamensis) after Intramuscular Administration at Three Different Dosages
Authors: Poapolathep Saranya , Klangkaew Narumol , Phaochoosak Napasorn , Wongwaipairoj Tara , Giorgi Mario , Chaiyabutr Narongsak , Trott Darren J. , Poapolathep Amnart
Published: July 2020

To date, the necessary pharmacokinetic information has been limited to establish suitable therapeutic plans for freshwater crocodiles. Therefore, this study was conducted to evaluate the pharmacokinetic profile of the oxytetracycline long-acting formulation (OTC-LA) in the freshwater crocodile, Crocodylus siamensis, following a single intramuscular (i.m.) administration at three different dosages of 5, 10 and 20 mg/kg body weight (b.w.). Blood samples were collected at assigned times up to 216 h after i.m. administration at the three different dosages. The plasma concentrations of OTC were measured using a validated liquid chromatography tandem-mass spectrometry (LC-MS/MS) method. The Cmax (± SD) values of OTC were 2.15 ± 0.51 µg/mL, 7.68 ± 1.08 µg/mL and 17.08 ± 2.09 µg/mL at doses of 5, 10 and 20 mg/kg b.w., respectively. The elimination half-life values were 33.59 ± 2.51 h, 38.42 ± 5.47 h and 38.04 ± 1.98 h at dosages of 5, 10 and 20 mg/kg b.w., respectively. Based on the pharmacokinetic data, the pharmacokinetic/pharmacodynamic (PK/PD) index, the susceptibility break-point and plasma protein binding, a dosage once every two weeks of 10 mg/kg b.w. OTC intramuscularly might be suitable for initiating the treatment of susceptible bacterial infections in freshwater crocodiles. However, further PK/PD studies are warranted to confirm whether the dose rates used in this study can produce longer-term antimicrobial success for diseases caused by susceptible bacteria in freshwater crocodiles.
Dietary Mannan Oligosaccharides Modulate Gut Inflammatory Response and Improve Duodenal Villi Height in Post-Weaning Piglets Improving Feed Efficiency
Authors: Agazzi Alessandro , Perricone Vera , Omodei Zorini Fabio , Sandrini Silvia , Mariani Elena , Jiang Xian-Ren , Ferrari Alessandra , Crestani Maurizio , Nguyen Thi Xuan , Bontempo Valentino , Domeneghini Cinzia , Savoini Giovanni
Published: July 2020

The aim of this study was to evaluate the effects of mannan oligosaccharides (MOS) on gut health and performance in post-weaning piglets. In total, 40 piglets were divided into two experimental groups and fed a basal diet with (TRT) or without (CON) 0.2% mannan oligosaccharides for 35 days. Growth performance was determined weekly and faecal microbial composition on days 0, 14 and 35. On day 36, histometrical evaluations were performed on duodenal, jejunal, ileal, and colon samples. mRNA gene expression of inflammation-related genes was evaluated in samples of ileal Peyer’s patches (IPP). MOS administration improved feed efficiency in the last two weeks of the trial (p < 0.05), and a decreased clostridia content was found in faeces at day 14 (p = 0.05). TRT piglets showed increased duodenal villi height (p < 0.05), and reduced mRNA levels of Tumour Necrosis Factor α (p < 0.05) and Toll-Like Receptor 4 (p < 0.01) in IPP. Our results suggest beneficial effects of MOS supplementation on gut morphology and the expression of inflammation-related genes in post-weaning piglets, accompanied by increased feed efficiency.
Population Genetic Analysis of Ten Geographically Isolated Tibetan Pig Populations
Authors: Shang Peng , Li Wenting , Tan Zhankun , Zhang Jian , Dong Shixiong , Wang Kejun , Chamba Yangzom
Published: July 2020

Several geographically isolated populations of Tibetan pigs inhabit the high-altitude environment of the Tibetan Plateau. Their genetic relationships, contribution to the pool of genetic diversity, and their origin of domestication are unclear. In this study, whole-genome re-sequencing data from 10 geographically isolated Tibetan pig populations were collected and analyzed. Population genetic analyses revealed limited genetic differentiation among the Tibetan pig populations. Evidence from deleterious variant analysis indicated that population-specific deleterious variants were the major component of all mutational loci. Contribution to the meta-population was largest in the TT (Qinghai-Tibet Plateau) population, based on gene diversity or allelic diversity. Selective sweep analysis revealed numerous genes, including RXFP1, FZD1, OR1F1, TBX19, MSTN, ESR1, MC1R, HIF3A, and EGLN2 which are involved in lung development, hard palate development, coat color, hormone metabolism, facial appearance, and perception of smell. These findings increase our understanding of the origins and domestication of the Tibetan pig, and help optimize the strategy for their conservation.
Effects of Parenteral Supplementation with Minerals and Vitamins on Oxidative Stress and Humoral Immune Response of Weaning Calves
Authors: Mattioli Guillermo Alberto , Rosa Diana Esther , Turic Esteban , Picco Sebastián Julio , Raggio Santiago José , Minervino Antonio Humberto Hamad , Fazzio Luis Emilio
Published: July 2020

We aimed to evaluate the effects of injectable mineral and vitamin supplementation on weaning calves subjected to a low-stress (fence-line) weaning system. Seven-month-old Aberdeen Angus female calves (n = 40, 152 ± 11 kg body weight) from a selenium-deficient area of extensive cattle production on natural grass were randomly assigned to two groups (n = 20 each). One group received subcutaneous supplementation with copper, zinc, selenium, manganese and vitamins A and E (SG), and the other was given sterile saline solution (CG). The animals were supplemented twice, seven days before weaning (day −7) and on the day of weaning (day 0), and they were evaluated 30 (+30) and 60 (+60) days after weaning. Total antioxidant status (TAS), selenium-dependent glutathione peroxidase (GPx) activity, body weight, and average daily gain (ADG) were evaluated. Additionally, antibody titers were assessed prior to and after each immunization with a vaccine containing bovine herpes virus type 1 (BoHV-1). On day +30, body weight (p = 0.03) was higher in SG, whereas TAS (p = 0.02) and GPx (p = 0.0038) activity were lower in CG and remained constant in SG. Antibody titers increased in SG and CG following immunization, being higher in SG on days +30 and +60 (p < 0.05). In conclusion, parenteral supplementation of minerals and vitamins with antioxidant effects in a low-stress weaning system prevented the decrease in TAS and GPx activity, improved antibody response and had positive effects on body weight.
Preference by Donkeys and Goats among Five Mediterranean Forest Species: Implications for Reducing Fire Hazard
Authors: Bartolomé Jordi , Miró Jordi , Panadès Xavier , Broncano Maria José , Plaixats Josefina , Rigau Teresa , Milán Maria José , Baraza Elena
Published: July 2020

During the second half of the 20th century, European countries experienced an increase in their forest area due to the global change. Consequently, there has been an increase in large forest fires, mainly in the Mediterranean basin, and this has forced the development of several types of prevention programs. One of them is the control of the understory by livestock. In this sense, browsing with a combination of donkeys and goats could be a good option, as both animals usually feed on forest species. However, little is known about their preferences for the key species of the Mediterranean forest. Using a cafeteria test, the preferences and consumption of both animals have been determined for five typical species of the Mediterranean forest, such as Quercus ilex, Pinus halepensis, Phillyrea latifolia, Rubus ulmifolius, and Brachypodium retusum. Results showed that donkeys and goats could act complementarily in the reduction of the fuel biomass of forests. Donkeys appear to act more on fine fuel, such as B. retusum, and goats on the more pyrophyte species, in this case P. halepensis. In addition, given that donkeys are at severe risk of extinction in Europe, this role of providing ecosystem services could contribute to their conservation. Despite this study only showing that goats and donkeys would consume all five presented plant species and that there are some differences in consumption during a short-term test, it constitutes a useful first step for conservation and fire prevention in the Mediterranean forests.
Post-Antibiotic Effect of Ampicillin and Levofloxacin to Escherichia coli and Staphylococcus aureus Based on Microscopic Imaging Analysis
Authors: Proma Farjana Hanif , Shourav Mohiuddin Khan , Choi Jungil
Published: July 2020

Post-antibiotic effect (PAE) is the continued suppression of bacterial growth following a limited exposure to an antimicrobial agent. The presence of PAE needs consequential consideration in designing antibiotic dosage regimens. To understand the behavior of bacteria, PAE provides information on how long antibiotics are applied to the bacteria. Conventional methods of measuring PAE depend on population detection and have limitations for understanding the individual behavior of bacteria. To observe the PAE, we utilized an imaging technique with the use of microscopy. Here, we discuss the microscopic image analysis system we used to study the PAE at a single-colony level. The size and number of colonies of bacteria were measured prior to and following antibiotic removal. We could count a single colony, see the development of the settlement prior to and following exposure of antibiotics and track the colony by microscopy according to the incubation time and the image processed by our own image processing program. The PAE of antibiotics was quantified by comparing bacteria size and number based on their exposure time. In our study, we discovered that the longer exposure of antibiotics causes the bacteria to be suppressed—even after washing the antibiotics from the solution. This finding suggests that microscopic imaging detection provides a new method for understanding PAE. In addition, the behavior of the cell in response to drugs and chemicals and their removal can be examined with the use of single colony analysis.
Study on the Ultimate Supporting Force of Shield Excavation Face Based on Anisotropic Strength Theory
Authors: Dai Chunquan , Sui Hongtao , Ma Chao
Published: July 2020

The determination of the ultimate supporting force of the shield excavation face is an important problem to be solved in shield construction. Considering that the tunnel burial depth ratio has a significant effect on the instability mode of the excavation face, the classic “wedge-prism” limit equilibrium model is improved. Based on the rotation effect of principal stress axis, the Casagrande anisotropic strength equation is introduced into the modified limit equilibrium model of “wedge-prism”, and then the limit equilibrium solution of the ultimate supporting force of shield excavation face in anisotropic soil is deduced. Finally, the influence of each calculation parameter on the ultimate supporting force is analyzed by examples. The research results show that the results of the modified “wedge-prism” calculation model proposed in this paper are slightly larger than those of the centrifugal test. If the influence of the instability mode of excavation face and the anisotropy of soil strength on ultimate supporting force of the shield excavation face is not taken into account, the calculation result will be unsafe. The limit supporting force of shield tunnel excavation surface has a simple linear relationship with the anisotropy ratio. When the anisotropy ratio is greater than 1, the ultimate supporting force of shield excavation face decreases first and then tends to be stable with an increase in the buried depth ratio. When the anisotropy ratio is less than 1, the law is reversed. The more obvious the anisotropy of soil strength, the greater the rate of change of ultimate supporting force. The limit supporting force of the shield excavation face decreases linearly with the exertion of loosening earth pressure, linearly decreases with the increase in soil cohesion, and decreases nonlinearly with the increase in the angle of internal friction in soil. The relevant conclusions will provide theoretical guidance for controlling the reasonable chamber pressure of shield tunneling, and ensure the safety of construction.
Experimental Study of Forced Convective Heat Transfer in a Coiled Flow Inverter Using TiO2–Water Nanofluids
Authors: Arevalo-Torres Barbara , Lopez-Salinas Jose L. , García-Cuéllar Alejandro J.
Published: July 2020

The curved geometry of a coiled flow inverter (CFI) promotes chaotic mixing through a combination of coils and bends. Besides the heat exchanger geometry, the heat transfer can be enhanced by improving the thermophysical properties of the working fluid. In this work, aqueous solutions of dispersed TiO2 nanometer-sized particles (i.e., nanofluids) were prepared and characterized, and their effects on heat transfer were experimentally investigated in a CFI heat exchanger inserted in a forced convective thermal loop. The physical and transport properties of the nanofluids were measured within the temperature and volume concentration domains. The convective heat transfer coefficients were obtained at Reynolds numbers (NRe) and TiO2 nanoparticle volume concentrations ranging from 1400 to 9500 and 0–1.5 v/v%, respectively. The Nusselt number (NNu) in the CFI containing 1.0 v/v% nanofluid was 41–52% higher than in the CFI containing pure base fluid (i.e., water), while the 1.5 v/v% nanofluid increased the NNu by 4–8% compared to water. Two new correlations to predict the NNu of TiO2–water nanofluids in the CFI at Reynolds numbers of 1400 ≤ NRe ≤ 9500 and nanoparticle volume concentrations ranges of 0.2–1.0 v/v% and 0.2–1.5 v/v% are proposed.
Wireless Home Assistive System for Severely Disabled People
Authors: Wu Chung-Min , Chen Yeou-Jiunn , Chen Shih-Chung , Yeng Chia-Hong
Published: July 2020

A lot of people with severe disabilities such as amyotrophic lateral sclerosis, motor neuron diseases, cerebral palsy, stroke, and spinal cord injury with intubation always have different degrees of communication problems. Therefore, it is very important to develop an effective and easy use assistive communication system for the severely disabled. In this study, a wireless home assistive system (WHAS) with different types of assistive input accessories sensors, Morse code translator, and human machine interface is developed and tested to help the severely disabled communicate with people and machines. A Morse code translator is implemented as an assistive communication core device to facilitate the input of the severely disabled. For the proposed human–machine interfaces, personal computer-based alternative augmentative communication is developed for patients to communicate with other people easily. To promote the quality of life, the home appliance control interface is developed for the severely disabled to directly control the functions of home appliances by themselves. The experimental results showed that the proposed WHAS is practical and feasible. Therefore, the proposed approach can help severely disabled individuals effectively interact with their surroundings.
The Effect of Undersized Drilling on the Coronal Surface Roughness of Microthreaded Implants: An In Vitro Study
Authors: Cohen Omer , Moses Ofer , Gurevich Talia , Kolerman Roni , Becker Alina , Ormianer Zeev
Published: July 2020

This in-vitro study assessed the effect of an underdrilling implant placement protocol on the insertion torque, implant surface temperature and surface roughness (Sa) topography of the cervical microthreads of implants. Three groups of 25 implants (3.75 mm × 10 mm) were placed in osteotomies prepared in an artificial bone disc with final diameters of 3.65 mm according to the manufacturer’s instructions and in osteotomies prepared in accordance with an underdrilling protocol with final drill diameters of 3.2 and 2.8 mm (groups D3.65, D3.2, D2.8, respectively). Implants were inserted at a constant rate of 30 rpm. The surface temperature of the implants was measured with a thermal camera and temperature amplitude (Temp-Amp) was calculated by subtracting the room temperature from the measured implant surface temperature. Upon implant retrieval, coronal surface topography was assessed using a Nanofocus µsurf explorer and compared to a set of 25 new implants (control group). The differences between groups were compared using one-way ANOVA (p < 0.05). Significantly higher insertion torque, surface temperature values and significantly smaller average Sa values were measured in the implants inserted in undersized preparations. The highest temperature, insertion torque and Temp-Amp values and the largest decrease in Sa were measured in the D2.8 group. The lowest values were measured in the D3.65 group.
Size Determination of Polystyrene Sub-Microspheres Using Transmission Spectroscopy
Authors: Nguyen Tien Van , Pham Linh The , Bui Khuyen Xuan , Nghiem Lien Ha Thi , Nguyen Nghia Trong , Vu Duong , Do Hoa Quang , Vu Lam Dinh , Nguyen Hue Minh
Published: July 2020

Nano/micro polystyrene (PS) beads have found many applications in different fields spanning from drug delivery, bio-diagnostics, and hybrid plasmonics to advanced photonics. The sizes of the PS beads are an important parameter, especially in plasmonic and photonic experiments. In this work, we demonstrate a quick and straightforward method to estimate the diameters of sub-microspheres (0.2 μm to 0.8 μm) using the transmission spectra of a close-packed monolayer of polystyrene beads on glass or quartz substrates. Experimental transmission spectra of the PS monolayers were verified against finite-difference time-domain (FDTD) simulation and showed good agreement. The effects of the substrates on the transmission spectra and, hence, the accuracy of the method were also studied by simulation, which showed that common transparent substrates only cause minor deviation of the PS bead sizes calculated by the proposed method.
A New Method for Determining the Degree of Controllability of State Variables for the LQR Problem Using the Duality Theorem
Authors: Zhang Lifei , Neusypin Konstantin Avenirovich , Selezneva Maria Sergeevna
Published: July 2020

The control performance of a dynamic system can be checked by the degree of controllability. In this work, we present a new method for determining the degree of observability of state variables for the linear quadratic optimal estimation (LQE) problem. We carried out the calculation of the degree of controllability for the linear quadratic optimal control (LQR) problem using a duality theorem. Compared with the traditional measures of controllability such as determinant, trace, and maximal eigenvalue of the inverse controllability Gramian, the proposed degree of controllability was developed for each state variable and takes into account both the controllability Gramian and the cost function. The new method is convenient to apply to LQR problem. In the numerical simulation, we determined the influence of the model parameters on the degree of controllability. Besides that, we analyzed the degree of controllability, which gives an insight into the relationship between the system model design and the control performance.
Dynamic Viscosity and Transverse Ultrasonic Attenuation of Engineering Materials
Authors: Ono Kanji
Published: July 2020

In this paper, ultrasonic attenuation of the transverse waves of engineering materials is evaluated, covering metals, ceramics, polymers, fiber-reinforced plastics, and rocks. After verifying experimental methods, 273 measurements are conducted and their results are tabulated. Fifty of the tests are for the longitudinal mode. Attenuation behavior is determined over broadband spectra. The attenuation spectra are characterized in four patterns, with 2/3 of the tests showing linear frequency dependence and another ¼ linear spectrum plus Rayleigh scattering (Mason-McSkimin relation). The longitudinal and transverse damping factors varied from 0.004 to 0.065, which are 1/3 to 5 times those of polymethyl methacrylate, suggesting that almost all the engineering materials tested may be viscoelastic. The present test results make the term dynamic viscosity more appropriate for exploring the underlying processes. The observed results were compared between the longitudinal and transverse modes and among similar material types. In more than a half of the tests, the transverse attenuation coefficients were higher than the corresponding longitudinal attenuation coefficients by 1.5× or more. Some material groups had similar attenuation coefficients for the two modes. Since the physical basis for viscous damping is hardly understood, especially in hard solids, further studies from new angles are keenly desired. This collection of new attenuation data will be of value for such works. Practically, this will assist in materials selection and in designing structural health monitoring and non-destructive inspection protocols.
Air Quality Levels and Health Risk Assessment of Particulate Matters in Abuja Municipal Area, Nigeria
Authors: Wambebe Nathaniel Mopa , Duan Xiaoli
Published: August 2020

This study focuses on assessing the health risk by particulate matter (PM) inhalation within the Abuja municipal area, Nigeria. Particulate matters (PM2.5 and PM10), HCHO and VOCs were collected by A handheld portable smart air quality detector BR-SMART-126. A hybrid single-particle Lagrangian integrated trajectory (HYSPLIT) model for backward trajectory was applied to tract the air flow (transportation) and potential sources. Health risk was estimated by comparing with the air quality index (AQI) stipulated by the World Health Organization (WHO). The result shows that the daily averaged concentrations of PM2.5 varied from 15.30 µg/m3 to 70.20 µg/m3. The top four most-polluted locations (Locations 10, 14, 17 and 18) of the twenty locations were found to be above the acceptable (25 µg/m3) AQI limit stipulated by WHO, which all fell far under the unhealthy AQI value index level. In general, business/commercial locations had the highest PM2.5 level followed by transport/market, offices/mixed use and residential. The results from the backwards trajectories show that the source of local particles for the four most-polluted locations is long-range air transport originating from the Atlantic Ocean. The results of the health-risk assessment implies that for PM2.5, the AQI varied from 73.2 to 280.8 in this assessment. Based on this, the population of workers within the business location are at health risk based on the relatively poor air quality in these areas—especially location 10 and 17. Based on these findings, it is recommended that the regulatory and enforcement agency needs to develop a more robust monitoring mechanism, regulations and enforcement. Furthermore, there is need for a national drive on renewable energy, clean energy for business/commercial district to help reduce fumes from generators and to form cleaner air initiatives in order to ensure a safe environment to live in as well as reduce particulate matters in the city.
Changes Detected in Five Bioclimatic Indices in Large Romanian Cities over the Period 1961–2016
Authors: Banc Ștefana , Croitoru Adina-Eliza , David Nicoleta Afrodita , Scripcă Andreea-Sabina
Published: August 2020

Bioclimatic indices are very important tools to evaluate the thermal stress of the human body. The aims of this study were to analyze the general bioclimatic conditions in ten big cities in Romania and to find out if there has been any change in five bioclimatic indices over a 56-year period: 1961–2016. The indices considered were: equivalent temperature, effective temperature, cooling power, universal thermal climate index and temperature-humidity index. They were calculated based on the daily meteorological data of air temperature, relative humidity, and wind speed recorded in 10 weather stations in Romania: Bucharest-Băneasa, Botoșani, Cluj-Napoca, Constanța, Craiova, Galați, Iași, Oradea, Sibiu and Timișoara. The features investigated for trend detection consisted of the frequency and length of the occurrence period for each class and for each index. The test used for trend detection was Mann-Kendall and the magnitude of the trend (the slope) was calculated by employing Sen’s slope method. The main results are based on frequency analysis. Three indices showed comfort class as dominant whereas the other two indicated cold stress conditions as dominant in the area. There was a shift from the cold stress conditions to the warm and hot ones for all the indices. The most stressful conditions for hot extremes did not indicate significant change. The climate in the big cities of Romania became milder during the cold season and hotter during the warm period of the year. The analysis of the length of each thermal class indicated mainly longer occurrence periods during the year for comfortable or warm stress classes.
Unravelling the Role of miR-20b-5p, CCNB1, HMGA2 and E2F7 in Development and Progression of Non-Small Cell Lung Cancer (NSCLC)
Authors: Arora Shweta , Singh Prithvi , Rahmani Arshad Husain , Almatroodi Saleh A. , Dohare Ravins , Syed Mansoor Ali
Published: August 2020

Lung cancer is a prime cause of worldwide cancer deaths, with non-small cell lung cancer (NSCLC) as a frequent subtype. Surgical resection, chemotherapy are the currently used treatment methods. Delayed detection, poor prognosis, tumor heterogeneity, and chemoresistance make them relatively ineffective. Genomic medicine is a budding aspect of cancer therapeutics, where miRNAs are impressively involved. miRNAs are short ncRNAs that bind to 3′UTR of target mRNA, causing its degradation or translational repression to regulate gene expression. This study aims to identify important miRNA-mRNA-TF interactions in NSCLC using bioinformatics analysis. GEO datasets containing mRNA expression data of NSCLC were used to determine differentially expressed genes (DEGs), and identification of hub genes-BIRC5, CCNB1, KIF11, KIF20A, and KIF4A (all functionally enriched in cell cycle). The FFL network involved, comprised of miR-20b-5p, CCNB1, HMGA2, and E2F7. KM survival analysis determines that these components may be effective prognostic biomarkers and would be a new contemplation in NSCLC therapeutics as they target cell cycle and immunosurveillance mechanisms via HMGA2 and E2F7. They provide survival advantage and evasion of host immune response (via downregulation of cytokines-IL6, IL1R1 and upregulation of chemokines-CXCL13, CXCL14) to NSCLC. The study has provided innovative targets, but further validation is needed to confirm the proposed mechanism.
Multiplex Soluble Biomarker Analysis from Pleural Effusion
Authors: Javadi Joman , Dobra Katalin , Hjerpe Anders
Published: July 2020

Malignant pleural mesothelioma (MPM) is a highly aggressive and therapy resistant pleural malignancy that is caused by asbestos exposure. MPM is associated with poor prognosis and a short patient survival. The survival time is strongly influenced by the subtype of the tumor. Dyspnea and accumulation of pleural effusion in the pleural cavity are common symptoms of MPM. The diagnostic distinction from other malignancies and reactive conditions is done using histopathology or cytopathology, always supported by immunohistochemistry, and sometimes also by analyses of soluble biomarkers in effusion supernatant. We evaluated the soluble angiogenesis related molecules as possible prognostic and diagnostic biomarkers for MPM by Luminex multiplex assay. Pleural effusion from 42 patients with malignant pleural mesothelioma (MPM), 36 patients with adenocarcinoma (AD) and 40 benign (BE) effusions were analyzed for 10 different analytes that, in previous studies, were associated with angiogenesis, consisting of Angiopoietin-1, HGF, MMP-7, Osteopontin, TIMP-1, Galectin, Mesothelin, NRG1-b1, Syndecan-1 (SDC-1) and VEGF by a Human Premixed Multi-Analyte Luminex kit. We found that shed SDC-1 and MMP-7 levels were significantly lower, whereas Mesothelin and Galectin-1 levels were significantly higher in malignant mesothelioma effusions, compared to adenocarcinoma. Galectin-1, HGF, Mesothelin, MMP-7, Osteopontin, shed SDC-1, NRG1-β1, VEGF and TIMP-1 were significantly higher in malignant pleural mesothelioma effusions compared to benign samples. Moreover, there is a negative correlation between Mesothelin and shed SDC-1 and positive correlation between VEGF, Angiopoietin-1 and shed SDC-1 level in the pleural effusion from malignant cases. Shed SDC-1 and VEGF have a prognostic value in malignant mesothelioma patients. Collectively, our data suggest that MMP-7, shed SDC-1, Mesothelin and Galectin-1 can be diagnostic and VEGF and SDC-1 prognostic markers in MPM patients. Additionally, Galectin-1, HGF, Mesothelin, MMP-7, Osteopontin, shed SDC-1 and TIMP-1 can be diagnostic for malignant cases.
Aberrant Membrane Composition and Biophysical Properties Impair Erythrocyte Morphology and Functionality in Elliptocytosis
Authors: Pollet Hélène , Cloos Anne-Sophie , Stommen Amaury , Vanderroost Juliette , Conrard Louise , Paquot Adrien , Ghodsi Marine , Carquin Mélanie , Léonard Catherine , Guthmann Manuel , Lingurski Maxime , Vermylen Christiane , Killian Theodore , Gatto Laurent , Rider Mark , Pyr dit Ruys Sébastien , Vertommen Didier , Vikkula Miikka , Brouillard Pascal , Van Der Smissen Patrick , Muccioli Giulio G. , Tyteca Donatienne
Published: July 2020

Red blood cell (RBC) deformability is altered in inherited RBC disorders but the mechanism behind this is poorly understood. Here, we explored the molecular, biophysical, morphological, and functional consequences of α-spectrin mutations in a patient with hereditary elliptocytosis (pEl) almost exclusively expressing the Pro260 variant of SPTA1 and her mother (pElm), heterozygous for this mutation. At the molecular level, the pEI RBC proteome was globally preserved but spectrin density at cell edges was increased. Decreased phosphatidylserine vs. increased lysophosphatidylserine species, and enhanced lipid peroxidation, methemoglobin, and plasma acid sphingomyelinase (aSMase) activity were observed. At the biophysical level, although membrane transversal asymmetry was preserved, curvature at RBC edges and rigidity were increased. Lipid domains were altered for membrane:cytoskeleton anchorage, cholesterol content and response to Ca2+ exchange stimulation. At the morphological and functional levels, pEl RBCs exhibited reduced size and circularity, increased fragility and impaired membrane Ca2+ exchanges. The contribution of increased membrane curvature to the pEl phenotype was shown by mechanistic experiments in healthy RBCs upon lysophosphatidylserine membrane insertion. The role of lipid domain defects was proved by cholesterol depletion and aSMase inhibition in pEl. The data indicate that aberrant membrane content and biophysical properties alter pEl RBC morphology and functionality.
Development of a Ratiometric Fluorescent Glucose Sensor Using an Oxygen-Sensing Membrane Immobilized with Glucose Oxidase for the Detection of Glucose in Tears
Authors: Duong Hong Dinh , Sohn Ok-Jae , Rhee Jong Il
Published: July 2020

Glucose concentration is an important parameter in biomedicine since glucose is involved in many metabolic pathways in organisms. Many methods for glucose detection have been developed for use in various applications, particularly in the field of healthcare in diabetics. In this study, ratiometric fluorescent glucose-sensing membranes were fabricated based on the oxygen levels consumed in the glucose oxidation reaction under the catalysis of glucose oxidase (GOD). The oxygen concentration was measured through the fluorescence quenching effect of an oxygen-sensitive fluorescent dye like platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) by oxygen molecules. Coumarin 6 (C6) was used as a reference dye in the ratiometric fluorescence measurements. The glucose-sensing membrane consisted of two layers: The first layer was the oxygen-sensing membrane containing polystyrene particles (PS) doped with PtP and C6 (e.g., PS@C6^PtP) in a sol–gel matrix of aminopropyltrimethoxysilane and glycidoxypropyltrimethoxysilane (GA). The second layer was made by immobilizing GOD onto one of three supporting polymers over the first layer. These glucose-sensing membranes were characterized in terms of their response, reversibility, interferences, and stability. They showed a wide detection range to glucose concentration in the range of 0.1 to 10 mM, but high sensitivity with a linear detection range of 0.1 to 2 mM glucose. This stable and sensitive ratiometric fluorescent glucose biosensor provides a reliable way to determine low glucose concentrations in blood serum by measuring tear glucose.
Distinguishing and Biochemical Phenotype Analysis of Epilepsy Patients Using a Novel Serum Profiling Platform
Authors: Hanas Jay , Hocker James , Vannarath Christian , Evangeline Betcy , Prabhakaran Vasudevan , Oommen Anna , Couch James , Anderson Michael , Rajshekhar Vedantam , Carabin Hélène , Drevets Douglas
Published: August 2020

Diagnosis of non-symptomatic epilepsy includes a history of two or more seizures and brain imaging to rule out structural changes like trauma, tumor, infection. Such analysis can be problematic. It is important to develop capabilities to help identify non-symptomatic epilepsy in order to better monitor and understand the condition. This understanding could lead to improved diagnostics and therapeutics. Serum mass peak profiling was performed using electrospray ionization mass spectrometry (ESI-MS). A comparison of sera mass peaks between epilepsy and control groups was performed via leave one [serum sample] out cross-validation (LOOCV). MS/MS peptide analysis was performed on serum mass peaks to compare epilepsy patient and control groups. LOOCV identified significant differences between the epilepsy patient group and control group (p = 10−22). This value became non-significant (p = 0.10) when the samples were randomly allocated between the groups and reanalyzed by LOOCV. LOOCV was thus able to distinguish a non-symptomatic epilepsy patient group from a control group based on physiological differences and underlying phenotype. MS/MS was able to identify potential peptide/protein changes involved in this epilepsy versus control comparison, with 70% of the top 100 proteins indicating overall neurologic function. Specifically, peptide/protein sera changes suggested neuro-inflammatory, seizure, ion-channel, synapse, and autoimmune pathways changing between epilepsy patients and controls.
Prognostic Value of CXCR2 in Breast Cancer
Authors: Boissière-Michot Florence , Jacot William , Fraisse Julien , Gourgou Sophie , Timaxian Colin , Lazennec Gwendal
Published: July 2020

The tumor microenvironment appears essential in cancer progression and chemokines are mediators of the communication between cancer cells and stromal cells. We have previously shown that the ligands of the chemokine receptor CXCR2 were expressed at higher levels in triple-negative breast cancers (TNBC). Our hypothesis was that CXCR2 expression could also be altered in breast cancer. Here, we have analyzed the potential role of CXCR2 in breast cancer in a retrospective cohort of 105 breast cancer patients. Expression of CXCR2, CD11b (a marker of granulocytes) and CD66b (a marker of neutrophils) was analyzed by immunohistochemistry on tumor samples. We demonstrated that CXCR2 stained mainly stromal cells and in particular neutrophils. CXCR2, CD11b and CD66b expression were correlated with high grade breast cancers. Moreover, TNBC displayed a higher expression of CXCR2, CD11b and CD66b than hormone receptor positive or Her2 positive tumors. High levels of CXCR2 and CD11b, but not CD66b, were associated with a higher infiltration of T lymphocytes and B lymphocytes. We also observed a correlation between CXCR2 and AP-1 activity. In univariate analyses, CXCR2, but not CD11b or CD66b, was associated with a lower risk of relapse; CXCR2 remained significant in multivariate analysis. Our data suggest that CXCR2 is a stromal marker of TNBC. However, higher levels of CXCR2 predicted a lower risk of relapse.
Survival from Cervical Cancer Diagnosed Aged 20–29 Years by Age at First Invitation to Screening in England: Population-Based Study
Authors: Castanon Alejandra , Tataru Daniela , Sasieni Peter
Published: July 2020

Age at which women are first invited to attend cervical screening in England has changed twice: in 2004, women under 25 years were no longer invited; and in 2012, first invitations were sent six months earlier (at age 24.5 years). Concomitantly, a dramatic increase in screen-detected cervical cancer was observed, and their survival had not been documented. Diagnoses of invasive cervical cancer at ages 20–29 years in 2006–2016 in England were followed until the end of 2018 for deaths. We estimated 8-year overall survival (OS) by International Federation of Gynecology and Obstetrics (FIGO) stage and age at first screening invitation. Overall and relative survival for stage IA cervical cancer for women diagnosed aged 20–29 years in England (n = 1905) was excellent at 99.8% (95% confidence intervals (CI): 99.4–99.9%) and 100% (95% CI: 99.7–100.1%), respectively. OS for stage IB cervical cancer (n = 1101) was 90.4% (95% CI: 88.3–92.2%). Survival from stage IB was worse for women diagnosed age 20–24 years compared to those diagnosed 25–29 years at diagnosis (p < 0.0001), but no difference was observed by age at first invitation for screening, p = 0.8575. OS for stage II (65.5%, 95% CI: 60.2–72.0%) and stage III+ (36.6%, 95% CI 28.4–44.7%) were poorer. Survival from stage I cervical cancer in young women in England is excellent: mortality in women with stage IA cancer is akin to that of the general population regardless of age at first invitation to screening.
Anti-Proliferative Effect of Statins Is Mediated by DNMT1 Inhibition and p21 Expression in OSCC Cells
Authors: Dongoran Rachmad Anres , Wang Kai-Hung , Lin Tsung-Jen , Yuan Ta-Chun , Liu Chin-Hung
Published: July 2020

Statins, also known as HMG-CoA reductase inhibitors, are a class of cholesterol-lowering drugs and their anti-cancer effects have been studied in different types of malignant diseases. In the present study, we investigated the anti-proliferative effects of statins, including cerivastatin and simvastatin, on oral squamous cell carcinoma (OSCC) cells. Our data showed that statins inhibited the proliferation of three OSCC cell lines in a dose-dependent manner and this growth inhibition was confirmed through G0/G1 cell cycle arrest. Accordingly, we found the upregulation of p21 and downregulation of cyclin-dependent kinases, including CDK2, CDK4, and CDK6, in the statin-treated cells. Importantly, we clearly showed that statins were able to inhibit the expression of DNA methyltransferase 1 (DNMT1) and further promote the expression of p21. Taken together, our data demonstrated that the anti-proliferative effect of statins is mediated by suppressing DNMT1 expression, thus promoting p21 expression and leading to G0/G1 cell cycle arrest in OSCC cells.
TrkB Inhibits the BMP Signaling-Mediated Growth Inhibition of Cancer Cells
Authors: Kim Min Soo , Jin Wook
Published: July 2020

We have previously observed that tropomyosin receptor kinase B (TrkB) induces breast cancer metastasis by activating both the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) and phosphatidylinositol-3-Kinase (PI3K)/AKT signaling pathways and inhibiting runt-related transcription factor 3 (RUNX3) and kelch-like ECH-associated protein 1 (KEAP1). These studies indicated that TrkB expression is crucial to the pathogenesis of breast cancer. However, how TrkB regulates bone morphogenetic protein (BMP) signaling and tumor suppression is largely unknown. Herein, we report that TrkB is a key regulator of BMP-mediated tumor suppression. TrkB enhances the metastatic potential of cancer cells by promoting cell anchorage-independent growth, migration, and suppressing BMP-2-mediated growth inhibition. TrkB inhibits the BMP-mediated activation of SMAD family member 1 (SMAD1) by promoting the formation of the TrkB/BMP type II receptor complex and suppresses RUNX3 by depleting BMP receptor I (BMPRI) expression. In addition, the knockdown of TrkB restored the tumor-inhibitory effect of BMP-2 via the activation of SMAD1. Moreover, the TrkB kinase activity was required for its effect on BMP signaling. Our study identified a unique role of TrkB in the regulation of BMP-mediated growth inhibition and BMP-2-induced RUNX3 expression.