bluuebunny/crossref_metadata_2025 huggingface dataset:
Artificial Small RNA-Based Silencing Tools for Antiviral Resistance in Plants
Authors: Cisneros Adriana E. , Carbonell Alberto
Published: May 2020

Artificial small RNAs (art-sRNAs), such as artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are highly specific 21-nucleotide small RNAs designed to recognize and silence complementary target RNAs. Art-sRNAs are extensively used in gene function studies or for improving crops, particularly to protect plants against viruses. Typically, antiviral art-sRNAs are computationally designed to target one or multiple sites in viral RNAs with high specificity, and art-sRNA constructs are generated and introduced into plants that are subsequently challenged with the target virus(es). Numerous studies have reported the successful application of art-sRNAs to induce resistance against a large number of RNA and DNA viruses in model and crop species. However, the application of art-sRNAs as an antiviral tool has limitations, such as the difficulty to predict the efficacy of a particular art-sRNA or the emergence of virus variants with mutated target sites escaping to art-sRNA-mediated degradation. Here, we review the different classes, features, and uses of art-sRNA-based tools to induce antiviral resistance in plants. We also provide strategies for the rational design of antiviral art-sRNAs and discuss the latest advances in developing art-sRNA-based methodologies for enhanced resistance to plant viruses.
Epigenetic Modifications: An Unexplored Facet of Exogenous RNA Application in Plants
Authors: Dalakouras Athanasios , Papadopoulou Kalliope K.
Published: May 2020

Exogenous RNA interference (exo-RNAi) is a powerful transgene-free tool in modern crop improvement and protection platforms. In exo-RNAi approaches, double-stranded RNAs (dsRNAs) or short-interfering RNAs (siRNAs) are externally applied in plants in order to selectively trigger degradation of target mRNAs. Yet, the applied dsRNAs may also trigger unintended epigenetic alterations and result in epigenetically modified plants, an issue that has not been sufficiently addressed and which merits more careful consideration.
Reactive Oxygen Species (ROS) and Nucleic Acid Modifications during Seed Dormancy
Authors: Katsuya-Gaviria Kai , Caro Elena , Carrillo-Barral Néstor , Iglesias-Fernández Raquel
Published: May 2020

The seed is the propagule of higher plants and allows its dissemination and the survival of the species. Seed dormancy prevents premature germination under favourable conditions. Dormant seeds are only able to germinate in a narrow range of conditions. During after-ripening (AR), a mechanism of dormancy release, seeds gradually lose dormancy through a period of dry storage. This review is mainly focused on how chemical modifications of mRNA and genomic DNA, such as oxidation and methylation, affect gene expression during late stages of seed development, especially during dormancy. The oxidation of specific nucleotides produced by reactive oxygen species (ROS) alters the stability of the seed stored mRNAs, being finally degraded or translated into non-functional proteins. DNA methylation is a well-known epigenetic mechanism of controlling gene expression. In Arabidopsis thaliana, while there is a global increase in CHH-context methylation through embryogenesis, global DNA methylation levels remain stable during seed dormancy, decreasing when germination occurs. The biological significance of nucleic acid oxidation and methylation upon seed development is discussed.
Evaluation of Allelopathic Activity of Chinese Medicinal Plants and Identification of Shikimic Acid as an Allelochemical from Illicium verum Hook. f.
Authors: Aniya  , Nomura Yoshihiro , Fuerdeng  , Appiah Kwame Sarpong , Fujii Yoshiharu
Published: May 2020

This study focused on the potential allelopathy of 50 species of Chinese medicinal plants, which are mainly distributed in the Xinjiang Uyghur Autonomous Region, Inner Mongolia, and Yunnan Province. The “sandwich method” was adopted and used for the screening for allelopathic potential among these plant species. Further phytotoxic evaluation of the candidate species was conducted by applying plant extracts to crops and weed species. The results of this study indicated that among the 50 medicinal plant species evaluated, the fruits of Illicium verum Hook. f. (star anise) showed the most significant allelopathic potential through the leaf leachates. Shikimic acid was identified to be the main bioactive compound (about 7% dry weight) in star anise by reversed-phase High Performance Liquid Chromatography (RP-HPLC) analysis. The phytotoxic bioassay indicated that both the crude extract of the Chinese star anise and the synthetic shikimic acid showed strong inhibitory activity on the radicle and hypocotyl growth of lettuce. The radicle growth inhibition of lettuce caused by the crude extract of star anise could be explained by the contribution of the biological activity of shikimic acid. In conclusion, shikimic acid could be a putative allelochemical in the fruits of Illicium verum and could be utilized in sustainable weed management.
Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton
Authors: Park Ji-Sun , Seong Zuh-Kyung , Kim Mi-Sun , Ha Jang-Ho , Moon Ki-Beom , Lee Hyo-Jun , Lee Hyeong-Kyu , Jeon Jae-Heung , Park Sang Un , Kim Hyun-Soon
Published: May 2020

Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established.
Bioactive Compounds and Aroma Profile of Some Lamiaceae Edible Flowers
Authors: Marchioni Ilaria , Najar Basma , Ruffoni Barbara , Copetta Andrea , Pistelli Luisa , Pistelli Laura
Published: May 2020

Edible flowers are consumed for their appearance, colours, nutritional and healthy properties, but the use is limited by the actual number of the species. Seven edible flowers of the Lamiaceae family (Ocimeae and Mentheae tribes) were investigated: Monarda didyma ‘Fireball’, Nepeta × faassenii ‘Six Hills Giant’, Ocimum basilicum ‘Blue Spice’, O. basilicum ‘Cinnamon’, Ocimum × citriodorum, Salvia discolor, and Salvia microphylla ‘Hot Lips’. Total soluble sugars, proteins, polyphenols, carotenoids, ascorbic acid and antioxidant activity were detected. The species of the Mentheae tribe contained higher sugar content than Ocimeae flowers, the opposite with regard to protein content. Ocimeae tribe flowers showed high polyphenols and carotenoids content. The Ocimeae tribe together with two specie of the Mentheae tribe showed an aroma profile dominated by sesquiterpene hydrocarbons (58.0% in S. discolor to 77.9% in Ocimum × citriodorum). Oxygenated monoterpenes prevailed in Nepeta and Monarda, also present in the essential oil of this latter species (84.5%). By contrast, Nepeta and S. discolor evidenced non-terpenes as the principal class (41.2% and 77.5%, respectively), while the oxygenated sesquiterpene was the main one in S. microphylla. The two varieties of Ocimum spp. showed oxygenated monoterpenes as the main class of volatiles.
DFT Prediction of Factors Affecting the Structural Characteristics, the Transition Temperature and the Electronic Density of Some New Conjugated Polymers
Authors: Vu Quoc-Trung , Tran Thi-Thuy-Duong , Nguyen Thuy-Chinh , Nguyen Thien Vuong , Nguyen Hien , Vinh Pham Van , Nguyen-Trong Dung , Dinh Duc Nguyen , Nguyen-Tri Phuong
Published: May 2020

Conjugated polymers are promising materials for various cutting-edge technologies, especially for organic conducting materials and in the energy field. In this work, we have synthesized a new conjugated polymer and investigated the effect of distance between bond layers, side-chain functional groups (H, Br, OH, OCH3 and OC2H5) on structural characteristics, phase transition temperature (T), and electrical structure of C13H8OS using Density Functional Theory (DFT). The structural characteristics were determined by the shape, network constant (a, b and c), bond length (C–C, C–H, C–O, C–S, C–Br and O–H), phase transition temperatures, and the total energy (Etot) on a base cell. Our finding shows that the increase of layer thickness (h) of C13H8OS–H has a negligible effect on the transition temperature, while the energy bandgap (Eg) increases from 1.646 eV to 1.675 eV. The calculation of bond length with different side chain groups was carried out for which C13H8OS–H has C–H = 1.09 Å; C13H8OS–Br has C–Br = 1.93 Å; C13H8OS–OH has C–O = 1.36 Å, O–H = 0.78 Å; C13H8OS–OCH3 has C–O = 1.44 Å, O–H =1.10 Å; C13H8OS–OC2H5 has C–O = 1.45 Å, C–C = 1.51Å, C–H = 1.10 Å. The transition temperature (T) for C13H8OS–H was 500 K < T < 562 K; C13H8OS–Br was 442 K < T < 512 K; C13H8OS–OH was 487 K < T < 543 K; C13H8OS–OCH3 was 492 K < T < 558 K; and C13H8OS–OC2H5 was 492 K < T < 572 K. The energy bandgap (Eg) of Br is of Eg = 1.621 eV, the doping of side chain groups H, OH, OCH3, and OC2H5, leads to an increase of Eg from 1.621 eV to 1.646, 1.697, 1.920, and 2.04 eV, respectively.
Essential Nanostructure Parameters to Govern Reinforcement and Functionality of Poly(lactic) Acid Nanocomposites with Graphene and Carbon Nanotubes for 3D Printing Application
Authors: Kotsilkova Rumiana , Ivanov Evgeni , Georgiev Vladimir , Ivanova Radost , Menseidov Dzhihan , Batakliev Todor , Angelov Verislav , Xia Hesheng , Chen Yinghong , Bychanok Dzmitry , Kuzhir Polina , Di Maio Rosa , Silvestre Clara , Cimmino Sossio
Published: May 2020

Poly(lactic) acid nanocomposites filled with graphene nanoplatelets (GNPs) and multiwall carbon nanotubes (MWCNTs) are studied, varying the filler size, shape, and content within 1.5–12 wt.%. The effects of the intrinsic characteristics of nanofillers and structural organization of nanocomposites on mechanical, electrical, thermal, and electromagnetic properties enhancement are investigated. Three essential rheological parameters are identified, which determine rheology–structure–property relations in nanocomposites: the degree of dispersion, percolation threshold, and interfacial interactions. Above the percolation threshold, depending on the degree of dispersion, three structural organizations are observed in nanocomposites: homogeneous network (MWCNTs), segregated network (MWCNTs), and aggregated structure (GNPs). The rheological and structural parameters depend strongly on the type, size, shape, specific surface area, and functionalization of the fillers. Consequently, the homogeneous and segregated network structures resulted in a significant enhancement of tensile mechanical properties and a very low electrical percolation threshold, in contrast to the aggregated structure. The high filler density in the polymer and the low number of graphite walls in MWCNTs are found to be determinant for the remarkable shielding efficiency (close to 100%) of nanocomposites. Moreover, the 2D shaped GNPs predominantly enhance the thermal conductivity compared to the 1D shaped MWCNTs. The proposed essential structural parameters may be successfully used for the design of polymer nanocomposites with enhanced multifunctional properties for 3D printing applications.
Tuning the Properties of Furandicarboxylic Acid-Based Polyesters with Copolymerization: A Review
Authors: Terzopoulou Zoi , Papadopoulos Lazaros , Zamboulis Alexandra , Papageorgiou Dimitrios G. , Papageorgiou George Z. , Bikiaris Dimitrios N.
Published: May 2020

Polyesters based on 2,5-furandicarboxylic acid (FDCA) are a new class of biobased polymers with enormous interest, both from a scientific and industrial perspective. The commercialization of these polymers is imminent as the pressure for a sustainable economy grows, and extensive worldwide research currently takes place on developing cost-competitive, renewable plastics. The most prevalent method for imparting these polymers with new properties is copolymerization, as many studies have been published over the last few years. This present review aims to summarize the trends in the synthesis of FDCA-based copolymers and to investigate the effectiveness of this approach in transforming them to a more versatile class of materials that could potentially be appropriate for a number of high-end and conventional applications.
Multifunctional and Transformable ‘Clickable’ Hydrogel Coatings on Titanium Surfaces: From Protein Immobilization to Cellular Attachment
Authors: Gevrek Tugce Nihal , Degirmenci Aysun , Sanyal Rana , Sanyal Amitav
Published: May 2020

Multifunctionalizable hydrogel coatings on titanium interfaces are useful in a wide range of biomedical applications utilizing titanium-based materials. In this study, furan-protected maleimide groups containing multi-clickable biocompatible hydrogel layers are fabricated on a titanium surface. Upon thermal treatment, the masked maleimide groups within the hydrogel are converted to thiol-reactive maleimide groups. The thiol-reactive maleimide group allows facile functionalization of these hydrogels through the thiol-maleimide nucleophilic addition and Diels–Alder cycloaddition reactions, under mild conditions. Additionally, the strained alkene unit in the furan-protected maleimide moiety undergoes radical thiol-ene reaction, as well as the inverse-electron-demand Diels–Alder reaction with tetrazine containing molecules. Taking advantage of photo-initiated thiol-ene ‘click’ reactions, we demonstrate spatially controlled immobilization of the fluorescent dye thiol-containing boron dipyrromethene (BODIPY-SH). Lastly, we establish that the extent of functionalization on hydrogels can be controlled by attachment of biotin-benzyl-tetrazine, followed by immobilization of TRITC-labelled ExtrAvidin. Being versatile and practical, we believe that the described multifunctional and transformable ‘clickable’ hydrogels on titanium-based substrates described here can find applications in areas involving modification of the interface with bioactive entities.
High Selective Composite Polyalkylmethylsiloxane Membranes for Pervaporative Removal of MTBE from Water: Effect of Polymer Side-chain
Authors: Borisov Ilya , Podtynnikov Ivan , Grushevenko Evgenia , Scharova Olga , Anokhina Tatiana , Makaev Sergey , Volkov Alexey , Volkov Vladimir
Published: May 2020

For the first time, the effect of the side-chain in polyalkylmethylsiloxane towards pervaporative removal of methyl tert-butyl ether (MTBE) from water was studied. The noticeable enhancement of separation factor during the pervaporation of 1 wt.% MTBE solution in water through the dense film (40–50 µm) can be achieved by substitution of a methyl group (separation factor 111) for heptyl (161), octyl (169) or decyl (180) one in polyalkylmethylsiloxane. Composite membrane with the selective layer (~8 µm) made of polydecylmethylsiloxane (M10) on top of microfiltration support (MFFK membrane) demonstrated MTBE/water separation factor of 310, which was 72% greater than for the dense film (180). A high separation factor together with an overall flux of 0.82 kg·m−2·h−1 allowed this M10/MFFK composite membrane to outperform the commercial composite membranes. The analysis of the concentration polarization modulus and the boundary layer thickness revealed that the feed flow velocity should be gradually increased from 5 cm·s−1 for an initial solution (1 wt.% of MTBE in water) to 13 cm·s−1 for a depleted solution (0.2 wt.% of MTBE in water) to overcome the concentration polarization phenomena in case of composite membrane M10/MFFK (Texp = 50 °C).
The Role of a Succinyl Fluorescein-Succinic Anhydride Grafted Atactic Polypropylene on the Dynamic Mechanical Properties of Polypropylene/Polyamide-6 Blends at the Polypropylene Glass Transition
Authors: García-Martínez Jesús-María , Collar Emilia P.
Published: May 2020

The present article adequately supports a twofold objective. On one hand, the study of the dynamic mechanical behavior of polypropylene/polyamide-6 blends modified by a novel compatibilizer was the objective. This was previously obtained by chemical modification of an atactic polypropylene polymerization waste. On the other hand, the accurate predictions of these properties in the experimental space scanned was the objective. As a novelty, this compatibilizer contains grafts rather than just maleated ones. Therefore, it consists precisely of an atactic polymer containing succinic anhydride (SA) bridges and both backbone and terminal grafted succinyl-fluorescein groups (SFSA) attached to the atactic backbone (aPP-SFSA). Therefore, it contains 6.2% of total grafting (2.5% as SA and 3.7% as SF), which is equivalent to 6.2 × 10−4 g·mol−1. This interfacial agent was uniquely designed and obtained by the authors themselves. Essentially, this article focuses on how the beneficial effect of both PA6 and aPP-SFSA varies the elastic (E’) and the viscous (E’’) behavior of the iPP/aPP-SFSA/PA6 blend at the iPP glass transition. Thus, we accurately measured the Dynamic Mechanical Analysis (DMA) parameters (E’, E’’) at this specific point considering it represents an extremely unfavorable scenario for the interfacial modifier due to mobility restrictions. Hence, this evidences the real interfacial modifications caused by aPP-SFSA to the iPP/PA6 system. Even more, and since each of the necessary components in the blend typically interacts with one another, we employed a Box–Wilson experimental design by its marked resemblance to the “agent-based models”. In this manner, we obtained complex algorithms accurately forecasting the dynamic mechanical behavior of the blends for all the composition range of the iPP/aPP-SFSA/PA6 system at the glass transition of iPP.
Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries
Authors: Dima Shinechimeg , Lee Yin-Yin , Watanabe Ikki , Chang Wei-Jen , Pan Yu-Hua , Teng Nai-Chia
Published: May 2020

Antimicrobials are important adjuncts in the treatment of caries and periodontitis. However, increased bacterial resistance and hypersensitivity reactions to commonly used antimicrobials have led to an increasing demand for safe and natural substances. The objective of this study was to investigate the antibacterial effects of ε-polylysine against oral pathogens Streptococcus mutans and Porphyromonas gingivalis. Broth dilution assay, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) analyses were performed to explore the antibacterial effect of ε-polylysine against S. mutans strain ATCC25175 and P. gingivalis strain ATCC332277. For the test solution, ε-polylysine was added to the bacterial suspension to prepare 0.125%, 0.25%, 0.5% and 1% ε-polylysine solutions diluted in broth medium. All four concentrations demonstrated complete inhibition of S. mutans and significantly reduced viable cell counts of P. gingivalis after 24 h. From starting inoculum of 9.15 log CFU/mL, P. gingivalis cell counts reduced to 4.01 log CFU/mL in the 0.125% ε-polylysine treatment group. SEM, CLSM, and the LIVE/DEAD bacterial assay of ε-polylysine application on P. gingivalis biofilm-dentin specimens revealed bacterial cell membrane disruption and irregular cell morphologies. The results indicated satisfactory antibacterial efficacy of ε-polylysine against P. gingivalis and S. mutans in liquid medium and as an application on biofilm-dentin specimens.
Synthesis of Phosphazene-Containing, Bisphenol A-Based Benzoxazines and Properties of Corresponding Polybenzoxazines
Authors: Sirotin Igor S. , Sarychev Igor A. , Vorobyeva Viktoria V. , Kuzmich Anastasia A. , Bornosuz Natalia V. , Onuchin Denis V. , Gorbunova Irina Yu. , Kireev Vyacheslav V.
Published: May 2020

With the aim of obtaining halogen-free polybenzoxazazines with reduced flammability, phosphazene-containing benzoxazines (PhBZ) were synthesized in a two-stage method. In the first stage of the reaction of hexachlorocycotriphosphazene with bisphenol A at molar ratios of 1:12, 1:16, and 1:24, respectively, mixtures of bisphenol and hydroxyaryloxycyclotriphosphazenes were obtained, which mainly contained P3N3[OC6H4C(CH3)3C6H4OH]6. In the second stage, when these mixtures interacted with aniline and an excess of paraformaldehyde in toluene at 80–90 °C, PhBZ containing 20–50% of the phosphazene component with Mw 1200–5800 were formed. According to 1H and 13C NMR spectroscopy, PhBZ contain a small amount of oligomeric compounds with Mannich aminomethylene bridges. With an increase of the content of the phosphazene component, the curing temperature of PhBZ decreases from 242 °C to 215 °C. Cured PhBZ samples with a phosphorus content of more than 1.5% have increased flammability resistance according to UL-94.
Polymer Labelling with a Conjugated Polymer-Based Luminescence Probe for Recycling in the Circular Economy
Authors: Kuřitka Ivo , Sedlařík Vladimír , Harea Diana , Harea Evghenii , Urbánek Pavel , Šloufová Ivana , Coufal Radek , Zedník Jiří
Published: May 2020

In this paper, we present the use of a disubstituted polyacetylene with high thermal stability and quantum yield as a fluorescence label for the identification, tracing, recycling, and eventually anti-counterfeiting applications of thermoplastics. A new method was developed for the dispersion of poly[1-phenyl-2-[p-(trimethylsilyl)phenyl]acetylene] (PTMSDPA) into polymer blends. For such purposes, four representative commodity plastics were selected, i.e., polypropylene, low-density polyethylene, poly(methyl methacrylate), and polylactide. Polymer recycling was mimicked by two reprocessing cycles of the material, which imparted intensive luminescence to the labelled polymer blends when excited by proper illumination. The concentration of the labelling polymer in the matrices was approximately a few tens ppm by weight. Luminescence was visible to the naked eye and survived the simulated recycling successfully. In addition, luminescence emission maxima were correlated with polymer polarity and glass transition temperature, showing a marked blueshift in luminescence emission maxima with the increase in processing temperature and time. This blueshift results from the dispersion of the labelling polymer into the labelled polymer matrix. During processing, the polyacetylene chains disentangled, thereby suppressing their intermolecular interactions. Moreover, shear forces imposed during viscous polymer melt mixing enforced conformational changes, which shortened the average conjugation length of PTMSDPA chain segments. Combined, these two mechanisms shift the luminescence of the probe from a solid- to a more solution-like state. Thus, PTMSDPA can be used as a luminescent probe for dispersion quality, polymer blend homogeneity, and processing history, in addition to the identification, tracing, and recycling of thermoplastics.
Coupling Microfluidics Data with Core Flooding Experiments to Understand Sulfonated/Polymer Water Injection
Authors: Tahir Muhammad , Hincapie Rafael E. , Langanke Nils , Ganzer Leonhard , Jaeger Philip
Published: May 2020

The injection of sulfonated-modified water could be an attractive application as it results in the formation of a mechanically rigid oil-water interface, and hence, possible higher oil recovery in combination with polymer. Therefore, detailed experimental investigation and fluid-flow analysis into porous media are required to understand the possible recovery mechanisms taking place. This paper evaluates the potential influence of low-salt/sulfate-modified water injection in oil recovery using a cross-analyzed approach of coupled microfluidics data and core flooding experiments. Fluid characterization was achieved by detailed rheological characterization focusing on steady shear and in-situ viscosity. Moreover, single and two-phase micromodels and core floods experiments helped to define the behavior of different fluids. Overall, coupling microfluidics, with core flooding experiments, confirmed that fluid-fluid interfacial interaction and wettability alteration are both the key recovery mechanisms for modified-water/low-salt. Finally, a combination of sulfate-modified/low-salinity water, with polymer flood can lead to ~6% extra oil, compared to the combination of polymer flood with synthetic seawater (SSW). The results present an excellent way to make use of micromodels and core experiments as a supporting tool for EOR processes evaluations, assessing fluid-fluid and rock-fluid interactions.
Current Trends in Simultaneous Determination of Co-Administered Drugs
Authors: Celia Christian , Di Marzio Luisa , Locatelli Marcello , Ramundo Piera , D’Ambrosio Francesca , Tartaglia Angela
Published: May 2020

Recently, high demand of high-throughput analyses with high sensitivity and selectivity to molecules and drugs in different classes with different physical-chemical properties—and a reduction in analysis time—is a principal milestone for novel methodologies that researchers are trying to achieve—especially when analytical procedures are applied to clinical purposes. In addition, to avoid high doses of a single drug that could cause serious side effects, multi-drug therapies are often used to treat numerous diseases. For these reasons, the demand for methods that allow the rapid analysis of mixed compounds has increased in recent years. In order to respond to these needs, new methods and instruments have been developed. However, often the complexity of a matrix can require a long time for the preparation and processing of the samples. Different problems in terms of components, types of matrices, compounds and physical-chemical complexity are encountered when considering drugs association profiles for quantitative analyses. This review addresses not only recently optimized procedures such as chromatographic separation, but also methods that have allowed us to obtain accuracy (precision and trueness), sensitivity and selectivity in quantitative analyses for cases of drug associations.
Optimization of the Classification Yard Location Problem Based on Train Service Network
Authors: Zhao Yinan , Lin Boliang
Published: May 2020

This paper investigates the problems of locating yards and allocating works of train formation among yards based on the train service network. It not only involves the modifications of the scales of existing yards, either improving, or downsizing or even demolishing, but also the determinations of building new yards in a given rail network. Besides, the train service network is also taken into consideration, so that the car-hour costs that are incurred from the reclassification and accumulation operations of railcars can be optimized simultaneously. The accumulation parameter setting is symmetrical on both directions of traffic flows. A binary integer programming model is first proposed for optimizing the train service network, the solution of which is employed as a benchmark of the further integrated optimization. Based on this, a nonlinear joint optimization model is developed aiming at striking a balance between the capital investment and car-hour consumptions, with the constraints of the reclassification capacities of yards and the number of sorting tracks, and multiple logical relations among decision variables. Corresponding linearization techniques are introduced for transforming the nonlinear models into the linear ones. Finally, an exact solving approach is presented with computational results that are based on a real-world based case to illustrate the efficacy of the linear model.
Learning Context-Aware Outfit Recommendation
Authors: Abugabah Ahed , Cheng Xiaochun , Wang Jianfeng
Published: May 2020

With the rapid development and increasing popularity of online shopping for fashion products, fashion recommendation plays an important role in daily online shopping scenes. Fashion is not only a commodity that is bought and sold but is also a visual language of sign, a nonverbal communication medium that exists between the wearers and viewers in a community. The key to fashion recommendation is to capture the semantics behind customers’ fit feedback as well as fashion visual style. Existing methods have been developed with the item similarity demonstrated by user interactions like ratings and purchases. By identifying user interests, it is efficient to deliver marketing messages to the right customers. Since the style of clothing contains rich visual information such as color and shape, and the shape has symmetrical structure and asymmetrical structure, and users with different backgrounds have different feelings on clothes, therefore affecting their way of dress. In this paper, we propose a new method to model user preference jointly with user review information and image region-level features to make more accurate recommendations. Specifically, the proposed method is based on scene images to learn the compatibility from fashion or interior design images. Extensive experiments have been conducted on several large-scale real-world datasets consisting of millions of users/items and hundreds of millions of interactions. Extensive experiments indicate that the proposed method effectively improves the performance of items prediction as well as of outfits matching.
On the Digital Pontryagin Algebras
Authors: Lee Sunyoung , Kim Yeonjeong , Lim Jeong-Eun , Lee Dae-Woong
Published: May 2020

In the current study, we explore digital homology modules, and investigate their fundamental properties on (pointed) digital images as one of the developments of symmetries. We also examine pointed digital Hopf spaces and base point preserving digital Hopf functions between pointed digital Hopf spaces with suitable digital multiplications, and explore the digital primitive homology classes, digital Pontryagin algebras on digital Hopf spaces as a symmetric phenomenon in mathematics and computer science.
On lp-Complex Numbers
Authors: Richter Wolf-Dieter
Published: May 2020

Dispensing with the common property of distributivity and replacing classical trigonometric functions with their l p -counterparts in Euler’s trigonometric representation of complex numbers, classes of l p -complex numbers are introduced and some of their basic properties are proved. The collection of all points that leave the l p -absolute value of each l p -complex number invariant under l p -complex numbers multiplication is shown to be a group of elements that have l p -absolute value one but not the symmetry group.
A Dual Channel Medium Access Control (MAC) Protocol for Underwater Acoustic Sensor Networks Based on Directional Antenna
Authors: Yang Jianmin , Qiao Gang , Hu Qing , Zhang Jiarong , Du Guangbin
Published: May 2020

Medium access control (MAC) protocol is an important link for achieving networks function in any wireless networks; an efficient and reliable MAC protocol is crucial for an effective underwater acoustic sensor networks (UASNs). Significant differences between UASNs and terrestrial sensor networks (TSNs) render the traditional MAC protocols applied on land inapplicable underwater. Existing MAC protocols for UASNs use the omnidirectional antenna, which wastes energy, restricts the network’s coverage range, and brings about unnecessary interferences in neighbor nodes. This paper proposes a dual channel MAC protocol for UASNs based on directional antenna (DADC-MAC), which increases the network coverage range, efficiently utilizes space, and reduces node interference compared to the omnidirectional antenna. The DADC-MAC protocol divides the channel into a data transmission channel and busy prompt message channel; the node uses the former to transmit the control frame and DATA package while the sending node and receiving node use the latter channel to inform the neighbor nodes of on-going communications to prevent DATA package collision. A neighbor discovery mechanism and directional network allocation vector are applied to resolve hidden terminal and deafness problems. Simulation results show that the DADC-MAC protocol could improve network throughput and reduce end-to-end delay, is efficient, performs well, and is well suited to both symmetrical and asymmetrical UASNs topology.
Bifurcations, Hidden Chaos and Control in Fractional Maps
Authors: Ouannas Adel , Almatroud Othman Abdullah , Khennaoui Amina Aicha , Alsawalha Mohammad Mossa , Baleanu Dumitru , Huynh Van Van , Pham Viet-Thanh
Published: May 2020

Recently, hidden attractors with stable equilibria have received considerable attention in chaos theory and nonlinear dynamical systems. Based on discrete fractional calculus, this paper proposes a simple two-dimensional and three-dimensional fractional maps. Both fractional maps are chaotic and have a unique equilibrium point. Results show that the dynamics of the proposed fractional maps are sensitive to both initial conditions and fractional order. There are coexisting attractors which have been displayed in terms of bifurcation diagrams, phase portraits and a 0-1 test. Furthermore, control schemes are introduced to stabilize the chaotic trajectories of the two novel systems.
Asymmetric Compensation of Reactive Power Using Thyristor-Controlled Reactors
Authors: Šapurov Martynas , Bleizgys Vytautas , Baskys Algirdas , Dervinis Aldas , Bielskis Edvardas , Paulikas Sarunas , Paulauskas Nerijus , Macaitis Vytautas
Published: May 2020

The thyristor-controlled reactor (TCR) compensator for smooth asymmetric compensation of reactive power in a low-voltage utility grid is proposed in this work. Two different topologies of compensator were investigated: topology based on a single-cored three-phase reactor and topology with separate reactors for every phase. The investigation of the proposed TCR compensator was performed experimentally using a developed experimental test bench for 12 kVAr total reactive power. The obtained results show that employment of separate reactors for every phase allows us to control the reactive power in every phase independently, and that the TCR compensator with three single-phase reactors is suitable for smooth and asymmetric compensation of reactive power in a low-voltage utility grid.
Firefly-Based Approaches of Image Recognition
Authors: Cocianu Catalina-Lucia , Stan Alexandru Daniel , Avramescu Mihai
Published: May 2020

The main aim of the reported work is to solve the registration problem for recognition purposes. We introduce two new evolutionary algorithms (EA) consisting of population-based search methods, followed by or combined with a local search scheme. We used a variant of the Firefly algorithm to conduct the population-based search, while the local exploration was implemented by the Two-Membered Evolutionary Strategy (2M-ES). Both algorithms use fitness function based on mutual information (MI) to direct the exploration toward an appropriate candidate solution. A good similarity measure is the one that enables us to predict well, and with the symmetric MI we tie similarity between two objects A and B directly to how well A predicts B, and vice versa. Since the search landscape of normalized mutual information proved more amenable for evolutionary computation algorithms than simple MI, we use normalized mutual information (NMI) defined as symmetric uncertainty. The proposed algorithms are tested against the well-known Principal Axes Transformation technique (PAT), a standard evolutionary strategy and a version of the Firefly algorithm developed to align images. The accuracy and the efficiency of the proposed algorithms are experimentally confirmed by our tests, both methods being excellently fitted to registering images.
Cylindrospermopsin-Microcystin-LR Combinations May Induce Genotoxic and Histopathological Damage in Rats
Authors: Díez-Quijada Leticia , Medrano-Padial Concepción , Llana-Ruiz-Cabello María , Cătunescu Giorgiana M. , Moyano Rosario , Risalde Maria A. , Cameán Ana M. , Jos Ángeles
Published: May 2020

Cylindrospermopsin (CYN) and microcystins (MC) are cyanotoxins that can occur simultaneously in contaminated water and food. CYN/MC-LR mixtures previously investigated in vitro showed an induction of micronucleus (MN) formation only in the presence of the metabolic fraction S9. When this is the case, the European Food Safety Authority recommends a follow up to in vivo testing. Thus, rats were orally exposed to 7.5 + 75, 23.7 + 237, and 75 + 750 μg CYN/MC-LR/kg body weight (b.w.). The MN test in bone marrow was performed, and the standard and modified comet assays were carried out to measure DNA strand breaks or oxidative DNA damage in stomach, liver, and blood cells. The results revealed an increase in MN formation in bone marrow, at all the assayed doses. However, no DNA strand breaks nor oxidative DNA damage were induced, as shown in the comet assays. The histopathological study indicated alterations only in the highest dose group. Liver was the target organ showing fatty degeneration and necrotic hepatocytes in centrilobular areas, as well as a light mononuclear inflammatory periportal infiltrate. Additionally, the stomach had flaking epithelium and mild necrosis of epithelial cells. Therefore, the combined exposure to cyanotoxins may induce genotoxic and histopathological damage in vivo.
The Effect of Zearalenone on the Cytokine Environment, Oxidoreductive Balance and Metabolism in Porcine Ileal Peyer’s Patches
Authors: Obremski Kazimierz , Trybowski Wojciech , Wojtacha Paweł , Gajęcka Magdalena , Tyburski Józef , Zielonka Łukasz
Published: May 2020

The aim of the present study was to determine the effect of zearalenone (ZEN), administered per os to gilts at doses equivalent to 50%, 100%, and 150% of no-observed-adverse-effect level (NOAEL) values for 14, 28, and 42 days during weaning, on changes in the parameters of the oxidoreductive balance, cytokine secretion, and basal metabolism in ileal Payer’s patches. Immunoenzymatic ELISA tests and biochemical methods were used to measure the concentrations of interleukin 1α, interleukin 1β, interleukin 12/23p40, interleukin 2, interferon γ, interleukin 4, interleukin 6, interleukin 8, tumor necrosis factor α, interleukin 10, transforming growth factor β, malondialdehyde, sulfhydryl groups, fructose, glucose, and proline, as well as the activity of peroxidase, superoxide dismutase and catalase. The study demonstrated that ZEN doses corresponding to 50%, 100%, and 150% of NOAEL values, i.e., 5 µg, 10 µg, and 15 µg ZEN/kg BW, respectively, have proinflammatory properties, exacerbate oxidative stress responses, and disrupt basal metabolism in ileal Payer’s patches in gilts.
Sevelamer Use in End-Stage Kidney Disease (ESKD) Patients Associates with Poor Vitamin K Status and High Levels of Gut-Derived Uremic Toxins: A Drug–Bug Interaction?
Authors: Dai Lu , Meijers Björn K. , Bammens Bert , de Loor Henriette , Schurgers Leon J. , Qureshi Abdul Rashid , Stenvinkel Peter , Evenepoel Pieter
Published: May 2020

Gut microbial metabolism is not only an important source of uremic toxins but may also help to maintain the vitamin K stores of the host. We hypothesized that sevelamer therapy, a commonly used phosphate binder in patients with end-stage kidney disease (ESKD), associates with a disturbed gut microbial metabolism. Important representatives of gut-derived uremic toxins, including indoxyl sulfate (IndS), p-Cresyl sulfate (pCS), trimethylamine N-oxide (TMAO), phenylacetylglutamine (PAG) and non-phosphorylated, uncarboxylated matrix-Gla protein (dp-ucMGP; a marker of vitamin K status), were analyzed in blood samples from 423 patients (65% males, median age 54 years) with ESKD. Demographics and laboratory data were extracted from electronic files. Sevelamer users (n = 172, 41%) were characterized by higher phosphate, IndS, TMAO, PAG and dp-ucMGP levels compared to non-users. Sevelamer was significantly associated with increased IndS, PAG and dp-ucMGP levels, independent of age, sex, calcium-containing phosphate binder, cohort, phosphate, creatinine and dialysis vintage. High dp-ucMGP levels, reflecting vitamin K deficiency, were independently and positively associated with PAG and TMAO levels. Sevelamer therapy associates with an unfavorable gut microbial metabolism pattern. Although the observational design precludes causal inference, present findings implicate a disturbed microbial metabolism and vitamin K deficiency as potential trade-offs of sevelamer therapy.
Biocontrol Agents and Natural Compounds against Mycotoxinogenic Fungi
Authors: Snini Selma Pascale , Mathieu Florence
Published: May 2020

Mycotoxins are toxic fungal secondary metabolites that contaminate food and feed [...]
Metagenomic Profiles of Antibiotic Resistance Genes in Activated Sludge, Dewatered Sludge and Bioaerosols
Authors: Han Il , Yoo Keunje
Published: May 2020

Wastewater treatment plants (WWTPs) have been considered hotspots for the development and dissemination of antibiotic resistance in the environment. Although researchers have reported a significant increase in bioaerosols in WWTPs, the associated bacterial taxa, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) remain relatively unknown. In this study, we have investigated the abundance and occurrences of ARGs and MGEs, as well as the bacterial community compositions in activated sludge (AS), dewatered sludge (DS) and bioaerosols (BA) in a WWTP. In total, 153 ARG subtypes belonging to 19 ARG types were identified by the broad scanning of metagenomic profiles obtained using Illumina HiSeq. The results indicated that the total occurrences and abundances of ARGs in AS and DS samples were significantly higher than those in BA samples (p < 0.05). However, some specific ARG types related to sulfonamide, tetracycline, macrolide resistance were present in relatively high abundance in BA samples. Similar to many other full-scale WWTPs, the Proteobacteria (58%) and Bacteroidetes (18%) phyla were dominant in the AS and DS samples, while the Firmicutes (25%) and Actinobacteria (20%) phyla were the most dominant in the BA samples. Although the abundance of genes related to plasmids and integrons in bioaerosols were two to five times less than those in AS and DS samples, different types of MGEs were observed in BA samples. These results suggest that comprehensive analyses of resistomes in BA are required to better understand the emergence of both ARGs and MGEs in the wastewater treatment process due to the significant increase of scientific attention toward bioaerosols effects.
Tillage Versus No-Tillage. Soil Properties and Hydrology in an Organic Persimmon Farm in Eastern Iberian Peninsula
Authors: Cerdà Artemi , Rodrigo-Comino Jesús , Yakupoğlu Tuğrul , Dindaroğlu Turgay , Terol Enric , Mora-Navarro Gaspar , Arabameri Alireza , Radziemska Maja , Novara Agata , Kavian Ataollah , Vaverková Magdalena Daria , Abd-Elmabod Sameh Kotb , Hammad Hafiz Mohkum , Daliakopoulos Ioannis N.
Published: May 2020

There is an urgent need to implement environmentally friendly agriculture management practices to achieve the Sustainable Goals for Development (SDGs) of the United Nations by 2030. Mediterranean agriculture is characterized by intense and millennia-old tillage management and as a consequence degraded soil. No-Tillage has been widely examined as a solution for soil degradation but No-Tillage relies more on the application of herbicides that reduce plant cover, which in turn enhances soil erosion. However, No-Tillage with weed cover should be researched to promote organic farming and sustainable agriculture. Therefore, we compare Tillage against No-Tillage using weed cover as an alternative strategy to reduce soil losses in persimmon plantations, both of them under organic farming management. To achieve these goals, two plots were established at “La Canyadeta” experimental station on 25-years old Persimmon plantations, which are managed with Tillage and No-Tillage for 3 years. A survey of the soil cover, soil properties, runoff generation and initial soil losses using rainfall simulation experiments at 55 mm h−1 in 0.25 m2 plot was carried out. Soils under Tillage are bare (96.7%) in comparison to the No-Tillage (16.17% bare soil), with similar organic matter (1.71 vs. 1.88%) and with lower bulk densities (1.23 vs. 1.37 g cm3). Tillage induces faster ponding (60 vs. 92 s), runoff (90 vs. 320 s) and runoff outlet (200 vs. 70 s). The runoff discharge was 5.57 times higher in the Tillage plots, 8.64 for sediment concentration and 48.4 for soil losses. We conclude that No-tillage shifted the fate of the tilled field after 3 years with the use of weeds as a soil cover conservation strategy. This immediate effect of No-Tillage under organic farming conditions is very promising to achieve the SDGs.
Compensating Water Service Interruptions to Implement a Safe-to-Fail Approach to Climate Change Adaptation in Urban Water Supply
Authors: Undurraga Rafael , Vicuña Sebastián , Melo Oscar
Published: May 2020

A city resilient to climate change is characterized by effectively responding to and recovering from the negative impacts of climate hazards. In the city of Santiago, Chile, extreme weather that can be associated with a nascent manifestation of climate change has caused high-turbidity events, repeatedly forcing the main water company to interrupt the supply of drinking water, affecting millions of people. This study proposes a transformative response to reduce harm from extreme events due to climate change. The traditional approach of increasing resilience through large infrastructure works can be complemented by one-off reductions in water use during emergencies, in exchange for economic compensation. This alternative seeks to transfer the individual responsibility of water companies to a collective one, where the community is an active agent that reduces damage in the face of extreme events resulting from climate change. In the assessment of this response, we used a choice experiment to estimate the minimum amount users are willing to accept in compensation for water service interruptions. The results show that willingness to accept compensation is significant (close to 0.6 USD/hour) and decreases when users have experienced additional unplanned interruptions. The aggregate cost of the compensation is lower than infrastructure investments required to avoid service interruptions under various future hypothetical hydroclimatic scenarios associated with climate change impacts. Therefore, compensation-based instruments for water service interruptions could be a more flexible and cost-effective alternative to infrastructure-based measures to cope with future climate hazards.