bluuebunny/crossref_metadata_2025 huggingface dataset:
Black Women Coaches in Community: Promising Practices for Mentorship in Canada
Authors: Joseph Janelle , McKenzie Alex I.
Published: April 2022

Mentorship programs have been shown to help under-represented women navigate their environments, but little research has been done on mentorship programs in sport coaching in Canada. The first of its kind in Canada, the Black Female Coach Mentorship Program (BFCMP) created by the Black Canadian Coaches Association in partnership with the Coaching Association of Canada caters to an historically excluded population: Black, Biracial, and Indigenous women coaches. The research aimed to understand the experiences of program participants to better inform policy, decision-making, and sustainability of the BFCMP. Through mentorship session observations, one-on-one semi-structured interviews with 15 of the 27 inaugural BFCMP mentors and mentees, and thematic analysis, we determined the ability to form a trusted community was a promising practice for coach mentorship programs. Our findings suggest that participants, the majority of whom were the only Black woman coach in their program/institution, benefit from mentorship because of the opportunities to help each other develop as leaders, build relationships to resist loneliness, and nurture resilience through community.
Indigenous Foods of India: A Comprehensive Narrative Review of Nutritive Values, Antinutrient Content and Mineral Bioavailability of Traditional Foods Consumed by Indigenous Communities of India
Authors: Kapoor Ridhima , Sabharwal Manisha , Ghosh-Jerath Suparna
Published: April 2022

India is endowed with several indigenous foods (IFs), that hold special cultural significance among local and ethnic caommunities, yet no attempts have been made till date to systematically compile their nutritive values. As per FAO's recent mandate on creation of “Global-Hub on Indigenous Food Systems,”IFs have received renewed global recognition for their potential to contribute to improved food security while enhancing biodiversity across the world. Hence, the useful properties of wild IFs require proper study and documentation in order to bridge the gap between scientific evidence generation and indigenous peoples' ancestral knowledge. For this purpose, we conducted a literature search in two scientific databases: PubMed and Google Scholar, between July 2020 and December 2021, to identify studies reporting nutritive values and/or antinutrient content of IFs (not included in Indian food composition database), consumed by Indian indigenous communities. A total of 52 Indian research articles were included, from which data was selected and extracted, to create a compendium on nutrient (n= 508) and antinutrient (n= 123) content of IFs, followed by computation of antinutrient-to-mineral molar ratios for 98 IFs to predict their mineral bioavailability. Maximum nutritive values were available for green leafy vegetables (n= 154), followed by other vegetables (n= 98), fruits (n= 66), cereals (n= 63), roots & tubers (n= 51) and nuts and legumes (n= 36). Several IFs seen to have better nutritional content than conventional foods and were found to be rich (i.e., >20% Indian recommended dietary allowances per reference food serve) in iron (54%), calcium (35%), protein (30%), vitamin C (27%), vitamin A (18%), zinc (14%) and folate (13%). Some IFs displayed high levels of antinutrients, however, anti-nutrient-to-mineral molar ratios were found to be low (for mainly leafy vegetables, other vegetables, and roots and tubers), thus indicating high mineral bioavailability. Hence, efforts are desirable to encourage the inclusion of these nutritionally superior IFs into the usual diets of indigenous communities. The IF database collated in our review can serve as a resource for researchers and policymakers to better understand the nutritional properties of region-specific IFs and promote them through contextual food-based interventions for improved dietary quality and nutrition outcomes in indigenous population of India.
The Current State of Vascular Surgery Presence in Bilibili Video Platform of China
Authors: Wu Haoliang , Wang Zhiwei , Li Mingxing , Sun Peng , Zhang Liwei , Zhang Cong , Wei Shunbo , Xie Boao , Lou Chunyang , Qiao Zhentao , Liu Yuanfeng , Bai Tao , Bai Hualong
Published: April 2022

Background

With the development of the Internet, more and more patients search for disease-related information on video platforms during the treatment process, and physicians also look for learning materials through these video platforms. Bilibili is one of the most popular video platforms in China. This study evaluated information on various interesting topics, and related surgical procedures searched through Bilibili.

Method

The Bilibili platform was independently queried for 12 common vascular diseases or related surgical procedures between October and November 2021 by two independent authors using the Baidu search engine. Information about the video and uploader was collected, and descriptive analyses of the overall and first-page results were performed.

Results

A total of 3,998 search results were retrieved by searching 12 vascular-related topics, of which 2,225 actual videos (55.7%) were finally confirmed to be related to medicine. Videos for the public accounted for 84.8% of these 2,225 videos. In addition, 50.5% of the video results were uploaded by vascular surgeons, 12.4% by other specialties, 17.7% by organizations, and 19.4% by other individuals. The total number of videos searched for varicose vein and peripheral vascular diseases was the largest, and the total number of leg amputation videos was the smallest. The largest number of videos for medical professionals was about pulmonary embolism, and the smallest was about leg amputation. On the first pages, 168 results (70.0%) were actually medically relevant, and only 7.7% of the videos were uploaded by vascular surgeons.

Conclusion

On the Bilibili platform, videos about vascular diseases are extensive but not comprehensive. The videos uploaded by vascular surgeons are rare, and the results searched are not precise. The online presence of vascular surgeons needs to be improved, which may partially solve the problem of low-quality videos due to the lack of strict management and censorship.
Genetic Alteration Analysis of IDH1, IDH2, CDKN2A, MYB and MYBL1 in Pediatric Low-Grade Gliomas
Authors: Barinfeld Orit , Zahavi Alon , Weiss Shirel , Toledano Helen , Michowiz Shalom , Goldenberg-Cohen Nitza
Published: April 2022

Objective

To investigate pediatric low-grade gliomas for alterations in IDH1, IDH2, CDKN2A, MYB, and MYBL1.

Materials and Methods

DNA and RNA were extracted from 62 pediatric gliomas. Molecular methods included PCR, RT-PCR, and RNA sequencing; Sanger sequencing was used for validation.

Results

Analysis for hotspot genetic alterations in IDH1 R132 and IDH2 R172 (45 and 33 samples) was negative in all cases. CDKN2A deletions were detected in exons 1 and 2 in 1 (pleomorphic xanthoastrocytoma) sample of 9 samples analyzed. Of 10 samples analyzed for MYB translocation, 4 each were positive for translocations with exon 2 and exon 3 of PCDHGA1. Six samples showed MYBL rearrangement. The lack of IDH1/2 genetic alterations is in accordance with the literature in pediatric tumors. Alterations in MYB, MYBL were recently reported to characterize diffuse grade II, but not grade I, gliomas.

Conclusion

We optimized methods for analyzing gene variations and correlated the findings to pathological grade. The high incidence of MYB and MYBL need further evaluation. We also compared DNA, RNA, and RNA sequencing results for fusion, translocation, and genetic alterations. More accurate identification of the underlying biology of pediatric gliomas has implications for the development of targeted treatment.
Treatment of Upper Lumbar Disc Herniation with a Transforaminal Endoscopic Technique
Authors: Wang Zuowei , Jian Fengzeng , Wu Hao , Wang Xingwen , Wang Kai , Duan Wanru , Liu Zhenlei , Chen Zan
Published: April 2022

Background

To investigate the clinical efficacy of percutaneous endoscopic transforaminal discectomy (PETD) in the treatment of upper lumbar disc herniation (LDH).

Methods

Twenty-two patients, 14 males and 8 females with ages ranging from 23 to 76 years, who had upper LDH and were treated with PETD from April 2015 to April 2020 in the Department of Neurosurgery of Xuanwu Hospital, were selected to evaluate the surgical efficacy by the visual analog scale (VAS) and Oswestry Disability Index (ODI).

Results

All patients underwent successful completion of PETD surgery. The operation time was 80.4 ± 18.0 min; intraoperative fluoroscopy was used 17.1 ± 8.7 times; and the hospital stay was 3.2 ± 0.6 days. The VAS scores were 7.9 ± 1.2, 2.3 ± 1.5, 2.2 ± 1.3, and 2.1 ± 1.0 before the operation, 1 day and 3 months after the operation, and during the last follow-up, respectively. The postoperative VAS score was significantly lower than that before the operation (P < 0.01). The ODI scores before and 3 months after the operation were 59.8 ± 16.8 and 15.3 ± 8.2, respectively; thus, the postoperative score was decreased (P < 0.01).

Conclusion

Upper lumbar discs have unique anatomical structures, and PETD is a safe and effective surgical method for the treatment of upper LDH.
Procalcitonin, Interleukin-6 and C-reactive Protein Levels Predict Renal Adverse Outcomes and Mortality in Patients with Acute Type A Aortic Dissection
Authors: Chen Xuelian , Zhou Jiaojiao , Fang Miao , Yang Jia , Wang Xin , Wang Siwen , Yang Lichuan
Published: April 2022

Background

Acute type A aortic coarctation (AAAD) is a highly deadly and serious life-threatening disease. The purpose of this study was to estimate the predictive value of peak procalcitonin, interleukin-6, and C-reactive protein levels on adverse renal outcomes and mortality in patients undergoing surgery for AAAD.

Methods

Perioperative peak PCT, CRP, and IL-6 levels were retrospectively collected in 331 patients hospitalized with AAAD from 2009 to 2021. The primary endpoints were AKI stage 2–3 and mortality. The receiver operating characteristic (ROC) curves were used to compare the predictive values of peak PCT, CRP, and IL-6 for different clinical outcomes. Multivariable logistic regression analysis was used to find risk factors for AKI and 30-day mortality.

Results

The incidence of AKI stage 2–3 following AAAD was 50.8% (168/331). The 30-day and overall mortality were significantly greater in the AKI 2–3 group than in the AKI 0–1 group (P = 0.000). ROC curve analysis showed that peak PCT, with an area under the ROC curve (AUC) of 0.712, was a more accurate predictor of adverse renal outcomes than peak IL-6 and CRP. Multivariable logistic regression analysis revealed that PCT > 0.39 ng/mL was an independent risk factor for AKI stage 2–3. Peak IL-6 > 259 pg/mL was found to be an independent risk factor for 30-day mortality.

Conclusion

In patients with surgery for AAAD, peak PCT provides a well-predictive indicator of AKI stage 2–3 and peak IL-6 indicates a favorable predictor of 30-day mortality.
Production of Triple-Gene (GGTA1, B2M and CIITA)-Modified Donor Pigs for Xenotransplantation
Authors: Xu Kaixiang , Yu Honghao , Chen Shuhan , Zhang Yaxuan , Guo Jianxiong , Yang Chang , Jiao Deling , Nguyen Tien Dat , Zhao Heng , Wang Jiaoxiang , Wei Taiyun , Li Honghui , Jia Baoyu , Jamal Muhammad Ameen , Zhao Hong-Ye , Huang Xingxu , Wei Hong-Jiang
Published: April 2022

Activation of human immune T-cells by swine leukocyte antigens class I (SLA-I) and class II (SLA-II) leads to xenograft destruction. Here, we generated the GGTA1, B2M, and CIITA (GBC) triple-gene-modified Diannan miniature pigs, analyzed the transcriptome of GBC-modified peripheral blood mononuclear cells (PBMCs) in the pig's spleen, and investigated their effectiveness in anti-immunological rejection. A total of six cloned piglets were successfully generated using somatic cell nuclear transfer, one of them carrying the heterozygous mutations in triple genes and the other five piglets carrying the homozygous mutations in GGTA1 and CIITA genes, but have the heterozygous mutation in the B2M gene. The autopsy of GBC-modified pigs revealed that a lot of spot bleeding in the kidney, severe suppuration and necrosis in the lungs, enlarged peripulmonary lymph nodes, and adhesion between the lungs and chest wall were found. Phenotyping data showed that the mRNA expressions of triple genes and protein expressions of B2M and CIITA genes were still detectable and comparable with wild-type (WT) pigs in multiple tissues, but α1,3-galactosyltransferase was eliminated, SLA-I was significantly decreased, and four subtypes of SLA-II were absent in GBC-modified pigs. In addition, even in swine umbilical vein endothelial cells (SUVEC) induced by recombinant porcine interferon gamma (IFN-γ), the expression of SLA-I in GBC-modified pig was lower than that in WT pigs. Similarly, the expression of SLA-II DR and DQ also cannot be induced by recombinant porcine IFN-γ. Through RNA sequencing (RNA-seq), 150 differentially expressed genes were identified in the PBMCs of the pig's spleen, and most of them were involved in immune- and infection-relevant pathways that include antigen processing and presentation and viral myocarditis, resulting in the pigs with GBC modification being susceptible to pathogenic microorganism. Furthermore, the numbers of human IgM binding to the fibroblast cells of GBC-modified pigs were obviously reduced. The GBC-modified porcine PBMCs triggered the weaker proliferation of human PBMCs than WT PBMCs. These findings indicated that the absence of the expression of α1,3-galactosyltransferase and SLA-II and the downregulation of SLA-I enhanced the ability of immunological tolerance in pig-to-human xenotransplantation.
Identification of Homozygous Regions With Adverse Effects on the Five Economic Traits of Duroc Pigs
Authors: Wang Shiyuan , Yang Jie , Li Guixin , Ding Rongrong , Zhuang Zhanwei , Ruan Donglin , Wu Jie , Yang Huaqiang , Zheng Enqin , Cai Gengyuan , Wang Xiaopeng , Wu Zhenfang
Published: April 2022

Runs of homozygosity (ROH) are widely used to estimate genomic inbreeding, which is linked to inbreeding depression on phenotypes. However, the adverse effects of specific homozygous regions on phenotypic characteristics are rarely studied in livestock. In this study, the 50 K SNP data of 3,770 S21 Duroc (American origin) and 2,096 S22 Duroc (Canadian origin) pigs were used to investigate the harmful ROH regions on five economic traits. The results showed that the two Duroc lines had different numbers and distributions of unfavorable ROHs, which may be related to the different selection directions and intensities between the two lines. A total of 114 and 58 ROH segments were found with significant adverse effects on the economic traits of S21 and S22 pigs, respectively. Serval pleiotropic ROHs were detected to reduce two or multiple phenotypic performances in two Duroc populations. Candidate genes in these shared regions were mainly related to growth, fertility, immunity, and fat deposition. We also observed that some ROH genotypes may cause opposite effects on different traits. This study not only enhances our understanding of the adverse effects of ROH on phenotypes, but also indicates that ROH information could be incorporated into breeding programs to estimate and control the detrimental effects of homozygous regions.
Non-native Nematode Ashworthius sidemi Currently Dominates the Abomasal Parasite Community of Cervid Hosts in the Czech Republic
Authors: Magdálek Jan , Bourgoin Gilles , Vadlejch Jaroslav
Published: April 2022

Ashworthius sidemi is an abomasal nematode typical for Asiatic cervids such as sambar (Rusa unicolor) or sika deer (Cervus nippon). This non-native parasite was introduced into Europe via sika deer in the late 19th and early 20th centuries. The current dynamic spread of this parasite amongst autochthonous wild cervids occurs independently of human activities, and A. sidemi has a negative impact on the health of wild ruminants and may pose a threat to the conservation of endangered wild ungulates and to livestock. This invasive parasite has been previously detected in the Czech Republic, but more accurate information on A. sidemi is required. Only limited information is generally available on the factors influencing the spread of abomasal nematodes in wild ruminants, so more information is necessary for planning effective strategies of parasite control. We therefore conducted a survey on the abomasal nematodes in cervids in both game reserves and hunting grounds across the Czech Republic, taking into account the hosts (species, age, sex) and environmental factors (monthly average temperature). The abomasa of 104 animals belonging to five cervid species originating from various locations of the country were collected. Data on host (species, sex, and age group) and the monthly average temperature in the region were obtained for each animal. The parasitological analyses indicated that 92% of the abomasa were infected by nematodes. Ashworthius sidemi was the most prevalent (72%) and abundant (80% of the total recovered individuals) nematode species and was detected in all cervid species except white-tailed deer. The intensity of A. sidemi was highest in roe deer (Capreolus capreolus) and fallow deer (Dama dama), but A. sidemi abundance did not depend substantially on the host or environmental factors. In contrast, the abundance of nematodes from the subfamily Ostertagiinae was influenced by the host species and temperature. Parasitic load was significantly higher in roe deer and during the warmer periods of the survey. We also detected another non-native nematode species, Spiculopteragia houdemeri. The results of our study suggest that the non-native nematode A. sidemi is now widespread amongst cervid hosts in the Czech Republic, probably due to the high sensitivity of autochthonous cervids to A. sidemi infections as well as adaptation of this parasite to the current climatic conditions of this country.
Case Report: Severe Anemia Associated With an Abomasal Fibrosarcoma in a Goat
Authors: Wagener Matthias Gerhard , Beythien Georg , Sterneberg Markus , Polifka Antje , Großmann Thekla , Baumgärtner Wolfgang , Ganter Martin
Published: April 2022

A 10-year-old female goat was presented to the clinic with lethargy, emaciation, and pale mucous membranes. Laboratory diagnosis revealed severe anemia with regenerative character as well as melena. Blood transfusions were administered, but the animal's condition continued to deteriorate, so it was euthanized. The main finding in the necropsy was an abomasal neoplasia with two metastases in the mesenterium which was positive for vimentin, but negative for smooth muscle actin and c-kit using immunohistochemistry, indicating a fibrosarcoma that might have contributed to gastrointestinal blood loss. Further pathological findings consisted of changes in the liver cells as well as a cervical leiomyoma. These findings illustrate that intestinal blood loss due to neoplasia should also be considered in older goats with anemia.
Effect of Organic Selenium-Enriched Yeast on Relieving the Deterioration of Layer Performance, Immune Function, and Physiological Indicators Induced by Heat Stress
Authors: Abbas Ahmed O. , Alaqil Abdulaziz A. , Mehaisen Gamal M. K. , El Sabry Mohamed I.
Published: April 2022

Heat stress (HS) induces deleterious effects on the performance of laying hens and causes economic losses for poultry industry. This study was carried out to investigate the organic effect of selenium-enriched yeast (SY) on relieving the performance, immunity and physiological deterioration induced by heat stress in laying hens. A total of 324, 28-week-old, Hy-Line Brown commercial chicken layers were randomly distributed into 4 treatments according to a 2 × 2 factorial design, with 9 hens × 9 replicates per treatment (n = 81). From 30 to 34 weeks of age, layers were exposed to 2 temperature treatments (the HS treatment groups): a thermoneutral temperature at 24°C and a heat stress at 35°C. Layers were further assigned into the 2 subgroups according to dietary supplementation with organic selenium-enriched yeast (the SY treatment groups) at either 0 or 0.4 mg/kg diet. Results indicated that all the aspects of the layer performance during the experimental period were impaired by exposure to HS, while SY supplementation improved the layer performance in both the HS and non-HS layers. Intestinal villi disruptions and liver necrotic hepatocytes were observed in the layers exposed to HS, while villi integrity and hepatocytic normality were enhanced by SY treatment. A significant (P < 0.05) decrease in the total leukocyte count, sheep red blood cell (SRBC) antibody titer, and T- and B-lymphocyte proliferation along with an increase in the heterophils/lymphocytes (H/L) ratio were observed in the HS layers compared to non-HS layers. On the contrary, SY treatment significantly (P < 0.05) improved the immune function traits in both the HS layers and non-HS layers. Furthermore, the SY treatment plays an important role in mitigating the oxidative stress and inflammation induced by HS, displaying lower levels of plasma corticosterone, lipid peroxidation, interleukin-1β, and tumor necrosis factor-α in HS layers supplemented with SY compared to HS layers without SY supplementation. These results conclude that addition of SY to the diet of laying hens could be applied as a potential nutritional approach to relieve the deterioration effects of heat stress on the immunity, physiological status, and productive performance of laying hens.
Occupational Factors Affecting Women Workers’ Sexual and Reproductive Health Outcomes in Oil, Gas, and Mining Industry: A Scoping Review
Authors: Razafimahefa Rina Hariniaina , Pardosi Jerico Franciscus , Sav Adem
Published: April 2022

Objectives: Globally, female workers workforce in Oil, Gas, and Mining (OGM) industry have increased significantly. The complexities of the OGM operations and the extensive exposure to workplace hazards potentially affect the health status of workers, including sexual and reproductive health (SRH) outcomes of female workers. Yet, the current state of knowledge on SRH issues in OGM contexts seems to be limited and fragmented. This scoping review aims to identify the occupational factors that influence women’s SRH outcomes in OGM industry.

Methods: This scoping review followed the Joanna Briggs Institute’s guidelines (PRISMA) and was conducted in five databases, including the citation chaining via Google Scholar and manual search through relevant organisations and Government websites. Sixteen articles met the inclusion criteria and were analysed.

Results: Despite the scarcity of evidence, chemical and physical are found to be the predominant factors greatly influencing women workers’ SRH outcomes in OGM. Most studies showed menstrual and cycle disorders, and risky pregnancy as key SRH issues. However, menstruation disorder was considerably linked with psychological and organisational factors.

Conclusion: This review suggests further empirical research on the relationship between OGM occupational hazards and women workers’ SRH. This will contribute to improvements in workplace safety legislations, measures, policies, and management systems taking into account women’s needs.
Going the Extra Mile (or Not): A Moderated Mediation Analysis of Job Resources, Abusive Leadership, Autonomous Motivation, and Extra-Role Performance
Authors: Parent-Lamarche Annick , Fernet Claude , Austin Stéphanie
Published: April 2022

Abusive leadership is particularly prevalent in nursing and it can have multiple adverse effects on performance at work. However, little research has examined whether and under what conditions abusive leadership may be detrimental to nurses’ extra-role performance. This cross-sectional study explores whether abusive leadership intensifies the effects of emotional job resources on autonomous motivation, a psychological mechanism that could be responsible for extra-role performance. Data were collected from dyads of registered French-Canadian nurses and their immediate supervisors (n = 99 dyads). The models were tested with path analysis using Mplus. Our results show that extra-role performance is positively associated with nurses’ job emotional resources and autonomous motivation, but negatively associated with abusive leadership. Nurses’ cynicism is also negatively associated with autonomous motivation. Importantly, the indirect relation between emotional resources and extra-role performance through autonomous motivation is moderated by abusive leadership, providing support for a moderated mediation effect. These results add to those supporting a similar moderated mediation mechanism to explain employee attitudes and demonstrate the relevance of self-determination theory in a work context. These findings reinforce the need to focus on the quality of leadership practices as well as interventions aimed at promoting the performance of nurses at work.
Plant-Response-Based Control Strategy for Irrigation and Environmental Controls for Greenhouse Tomato Seedling Cultivation
Authors: Fang Shih-Lun , Chang Ting-Jung , Tu Yuan-Kai , Chen Han-Wei , Yao Min-Hwi , Kuo Bo-Jein
Published: April 2022

Most existing greenhouse decision support systems only consider external environmental factors, such as soil and atmosphere, rather than plant response. A conceptual plant-response-based strategy for irrigation and environmental controls for tomato (Solanum lycopersicum) seedling cultivation in greenhouse operations was proposed. Because stomatal conductance (gsw) is a comprehensive indicator of plants, soil moisture, and atmospheric conditions, this study used gsw to design a conceptual system by employing factors affecting gsw as the key for decision-making. Logistic regression was performed with independent variables (i.e., temperature (Tair), vapor pressure deficit (VPD), and leaf–air temperature difference) to predict the gsw status. When the gsw status was “low,” the system entered into the environmental control component, which examined whether the VPD and the photosynthetic photon flux density (PPFD) were in the normal range. If the VPD and the PPFD were not in the normal range, the system would offer a suggestion for environmental control. Conversely, when both parameters were in the normal range, the system would determine that irrigation should be performed and the irrigation amount could be estimated by the evapotranspiration model. Thus, the strategy only considered leaf temperature, Tair, VPD, and PPFD, and the overall error rate to characterize gsw was below 13.36%.
Delineation of Physiological, Agronomic and Genetic Responses of Different Wheat Genotypes under Drought Condition
Authors: Shah Syed Muhammad Dawood Murtaza , Shabbir Ghulam , Malik Saad Imran , Raja Naveed Iqbal , Shah Zahid Hussain , Rauf Muhammad , Zahrani Yahya Al , Alghabari Fahad , Alsamadany Hameed , Shahzad Khurram , Yang Seung Hwan
Published: April 2022

Abioticstress such as drought is a potential threat posing a severe challenge to wheat production across the globe. The current study comparatively delineated the performance of elite Pakistani bread wheat genotypes at physiological (chlorophyll, canopy temperature, cell membrane percentage stability and leaf relative water content), agronomic (plant height, tillers plant−1, flag leaf area, spike length, spikelets spike−1, grains spike−1, grain yield spike−1, thousand grain weight and plant biomass) and genetic (TaDREB1A, TaGROS-A, TaLEA3, TaHSFA1a, TaWRKY44 and TaEXPA2) levels. Atri-replicate experiment was conducted in a two factorial arrangement using RCBD, and data were analyzed statistically using the computer-based programsStatistix8.1 and R-studio. In general, all wheat genotypes illustrated significant (p ≤ 0.05) alterations in physiological and agronomic traits under drought stress as compared to the control; however, this alteration was significantly (p ≤ 0.05) different among all genotypes owing to their varying genetic potential. Furthermore, these genotypes were evaluated for the extent of the association of physiological and agronomic traits using PCA, correlation and heatmap analysis, which proved statistically significant variation in the paired association of traits among all genotypes during drought stress as compared to the control. In addition, based on statistical evaluations, the genotypes Pakistan-13, Shahkar-13, AAS-11, Chakwal-86, Chakwal-50 and AUR-09 were found to be tolerant, while genotypes Anmol-97, Chakwal-97, Bhakkar-02 and BWP-97 were comparatively susceptible. Furthermore, these screened genotypes showed differential expression of drought-related genes, with relatively high expression in tolerant genotypes compared to susceptible genotypes. The current study concluded that physiological, agronomic and molecular characteristics are significantly interconnected, and these associations determine the end productivity of wheat genotypes during abiotic stress. Therefore, their integrated study can enhance the pace of wheat breeding for drought tolerance in the near future.
Metabolomic Profile of Citrus limon Leaves (‘Verna’ Variety) by 1H-NMR and Multivariate Analysis Technique
Authors: Melgarejo Pablo , Núñez-Gómez Dámaris , Martínez-Nicolás Juan José , Hernández Francisca , Martínez-Font Rafael , Lidón Vicente , García-Sánchez Francisco , Legua Pilar
Published: April 2022

The elaboration and definition of “metabolic fingerprints” can subsidize both the identification and determination of plant varieties, as well as the increase in knowledge about the responses and adaptations of plants to external and/or internal factors. The lemon tree (Citrus limon Burm.) is one of the most important crops in the Spanish southeast and is often consumed around the world. Although the study and characterization of its fruits are common due to its economic interest, its leaves are limited to specific functionalized studies related to the objective of the work (extraction of essential oils, stabilizing agent, aromatic extracts, etc.). So, this study aimed to identify the primary and secondary metabolites of Citrus limon Burm. (‘Verna’ variety) leaf samples cultivated under different conditions (three rootstocks and three culture media). In total, 19 metabolites were identified for all samples, of which 9 were amino acids, 5 organic acids, 3 sugars and 2 intermediate metabolites. The results pointed to a limited influence, both of the substrate and of the crop rootstock, on the metabolomic differentiation of lemon leaves. Knowledge and foliar metabolomic differentiation can offer important information that supports the application of crop foliar treatments but also helps in the management of diseases and pests.
Analysis on the Difference of Reconstructed Soil Moisture Content in a Grassland Open-Pit Mining Area of China
Authors: Wang Lingling , Li Yange , Zhang Jianjun , Qian Mingjie , Cao Yingui
Published: April 2022

To reveal the variability of different reconstructed soil ratios and different vegetation growth grades on the water holding capacity of recon-structed soil, the most suitable ratio of reconstructed soil materials was explored. Taking the inner dump of Shengli mining area in Inner Mongolia of China as the research area, the reconstructed soil of reclaimed land was investigated and sampled. One-way analysis of variance was used to analyze the difference of the moisture content of the reconstructed soil, and the reasons for the difference were discussed. The results showed that: (1) Among the different soil reconstructions, soil moisture content was higher when soil reconstructions were rock and soil stripping material: coal gangue: fly ash = 3:4:3. The soil moisture content of un-reclaimed land was mostly at a high level when the soil reconstruction method was rock and soil stripping material; and the soil moisture content was not at the highest level when the vegetation growth grade was higher. This indicates that it was not the case that the better the vegetation growth condition was, the higher the soil moisture content was. (2) In the case where the soil reconstruction method was rock and soil stripping material: coal gangue = 2:3, the soil moisture content of the reclaimed land decreased with the reduction of tillage frequency when the vegetation growth condition was optimal (vegetation growth grade was 4). (3) In the case where the soil reconstruction method was rock and soil stripping material: coal gangue: fly ash = 3:4:3, when the vegetation growth condition was better (vegetation growth grade was 3 and 4), the soil moisture content of the re-claimed land was highest when it was tilled once every 15 days. It was also found in combination with other soil reconstruction methods that it was not always the case that the higher the frequency of tillage, the higher the soil moisture content. The study of coal gangue, fly ash and rocky soil stripping as topsoil substitute materials with their different ratios on reconstructed soil provides support for the reclamation work in topsoil scarce mines and provides technical reference for the ecological restoration project of grassland open-pit mines in the same climate zone.
Soil Available Phosphorus Deficiency Reduces Boll Biomass and Lint Yield by Affecting Sucrose Metabolism in Cotton-Boll Subtending Leaves
Authors: Sun Miao , Li Pengcheng , Wang Ning , Zheng Cangsong , Sun Xuezhen , Dong Helin , Han Huimin , Feng Weina , Shao Jingjing , Zhang Yufeng
Published: April 2022

Soil available phosphorus (AP) deficiency and shortage of phosphate rocks limit cotton production in China. Therefore, pool-culture experiments were conducted in 2019 and 2020 using two cotton cultivars (CCRI-79, low-P tolerant; SCRC-28, low-P sensitive) under three soil AP levels (P0: 3 ± 0.5, P1: 6 ± 0.5, and P2 (control): 15 ± 0.5 mg kg−1) to ascertain the effect of soil AP on boll biomass and lint yield. P0 and P1 decreased the P concentration and net photosynthetic rate (Pn) of subtending leaves, thus, reducing boll biomass and lint yield. Additionally, soil AP deficiency decreased boll wall:boll, lint:boll, and lint:seed, and increased seed:boll ratio. Upper fruiting branch positions (FB9–12) had higher lint:seed ratio and proportion of the total lint yield under low soil AP. Moreover, soil AP deficiency also reduced the sucrose transformation rate (Tr) and activities of sucrose-metabolizing enzymes, such as ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco), sucrose phosphate synthase (SPS), and sucrose synthase (SuSy), while increased carbohydrate levels (soluble sugar, sucrose, and starch) and the activity of cytosolic fructose-1,6-bisphosphatase (cy-FBPase) in the subtending leaves. The sucrose and starch contents, cy-FBPase, and SPS activities of SCRC-28 were more sensitive to low soil AP than CCRI-79. Higher Tr and activities of initial Rubisco and SuSy in the subtending leaves improved boll biomass and lint yield.
Effect of Irrigation with Activated Water on Root Morphology of Hydroponic Rice and Wheat Seedlings
Authors: Yang Xueting , Fan Jun , Ge Jiamin , Luo Zhanbin
Published: April 2022

Raising yields and agricultural production efficiency is the primary goal of realizing modern agriculture. As a low-cost and environmentally friendly technology, activated water has the potential to increase crop yields and improve water and fertilizer use efficiency, but much research is still needed to make this technology widely available in the field. Hydroponic experiments were conducted to investigate the effects of magnetized water, aerated water, and magnetized aerated water on rice and wheat seedling and root growth. The results showed that aerated water irrigation significantly increased plant height by 5.1–9.6%, leaf area by 21.1%, and aboveground biomass by 14.8–16.3%, respectively. Aerated water irrigation also significantly promoted rice root biomass, maximum root length, total root length, total root surface area, and especially the proportion of roots less than 0.5 mm in diameter, indicating that aerated water enhances the growth of rice seedlings mainly by promoting root growth, especially fine root (D ≤ 0.5 mm) growth. The maximum root length and total root volume of wheat roots under magnetized water irrigation treatment were increased by 7.7–8.6% and 17.2%, respectively, resulting in a significant increase in aboveground dry biomass by 13.6%. Magnetized water and magnetized aerated water irrigation also promoted the growth of rice seedlings and roots. In contrast, aerated water and magnetized aerated water irrigation exhibited an inhibitory effect on the growth of wheat seedlings and roots. Therefore, activated water has different effects on different crops in hydroponics, and more research is needed in the future to determine the conditions for the application of activated water in agriculture.
Investigation of the Efficacy of Dithiothreitol and Glutathione on In Vitro Fertilization of Cryopreserved Large White Boar Semen
Authors: Ledwaba Mahlatsana Ramaesela , Mphaphathi Masindi Lottus , Thema Mamonene Angelinah , Pilane Cyril Mpho , Nedambale Tshimangadzo Lucky
Published: April 2022

The objectives of this study were to evaluate the properties of sperm motility and morphology under induced oxidative stress, compare the antioxidant capacity of dithiothreitol (DTT) and glutathione (GSH) following the cryopreservation of Large White boar semen, investigate the ability of cryopreserved Large White boar semen to fertilize the matured gilts oocytes and compare the efficacy of DTT and GSH antioxidants in improving the oocyte fertilization by cryopreserved Large White boar semen. The semen was collected from three Large White boars (ten ejaculates per boar) and transported (37 °C) to the laboratory. Semen freezing extenders were supplemented with 5 mM DTT, 5 mM GSH and a combination of 2.5 mM DTT + 2.5 mM GSH. A liquid nitrogen vapor method was used to freeze boar semen. Gilts’ ovaries were collected from the local abattoir and transported (37 °C) to the laboratory. The slicing method was used to retrieve the oocytes from the ovaries. Fresh semen and frozen-thawed semen were used for in vitro fertilization (IVF). For frozen-thawed semen, four treatments (control, 5 mM DTT, 5 mM GSH, and a combination of 2.5 mM DTT + 2.5 mM GSH) were used during IVF in order to evaluate the fertilizing ability of the antioxidants. The supplementation of 5 µM DTT to H2O2-treated semen significantly improved progressive motility (PM) by 14.82%. A combination of 2.5 mM DTT + 2.5 mM GSH treatment reduced percentage of sperm total motility (TM) and rapid motility (RAP) following thawing (p < 0.05). Fresh semen and a combination of 2.5 mM DTT + 2.5 mM GSH treatment recorded a higher percentage of zygotes with polyspermy (p < 0.05). The control treatment numerically recorded a high percentage of zygotes with 1 PN, while the 5 mM DTT treatment recorded a high percentage of zygotes with 2 PN.
Follicular Size Threshold for Ovulation Reassessed. Insights from Multiple Ovulating Dairy Cows
Authors: López-Gatius Fernando , Llobera-Balcells Mònica , Palacín-Chauri Roger , Garcia-Ispierto Irina , Hunter Ronald
Published: April 2022

In Bos. taurus cattle, follicular deviation to dominance begins when the selected ovulatory follicle reaches a mean diameter of 8.5 mm. The dominant follicle acquires the capacity to ovulate when it reaches a diameter of about 10 mm. In this study, data derived from 148 cows in estrus with one follicle of ovulatory size and another of 7–9 mm, reveal that the small follicle has the capacity to ovulate alone or with the dominant follicle; thus, giving rise to a single or twin pregnancy. This indicates that a follicle of deviation size may ovulate in the presence of a follicle of ovulatory size.
Hydroxyapatite/TiO2 Nanomaterial with Defined Microstructural and Good Antimicrobial Properties
Authors: Mirković Miljana , Filipović Suzana , Kalijadis Ana , Mašković Pavle , Mašković Jelena , Vlahović Branislav , Pavlović Vladimir
Published: April 2022

Due to the growing number of people infected with the new coronavirus globally, which weakens immunity, there has been an increase in bacterial infections. Hence, knowledge about simple and low-cost synthesis methods of materials with good structural and antimicrobial properties is of great importance. A material obtained through the combination of a nanoscale hydroxyapatite material (with good biocompatibility) and titanium dioxide (with good degradation properties of organic molecules) can absorb and decompose bacteria. In this investigation, three different synthesis routes used to prepare hydroxyapatite/titanium dioxide nanomaterials are examined. The morphology and semiquantitative chemical composition are characterized by scanning electron microscopy with energy dispersive X-ray analysis (SEM-EDX). The obtained materials’ phase and structural characterization are determined using the X-ray powder diffraction method (XRD). The crystallite sizes of the obtained materials are in the range of 8 nm to 15 nm. Based on XRD peak positions, the hexagonal hydroxyapatite phases are formed in all samples along with TiO2 anatase and rutile phases. According to SEM and TEM analyses, the morphology of the prepared samples differs depending on the synthesis route. The EDX analysis confirmed the presence of Ti, Ca, P, and O in the obtained materials. The IR spectroscopy verified the vibration bands characteristic for HAp and titanium. The investigated materials show excellent antimicrobial and photocatalytic properties.
Increased Circulating Levels of PCSK9 and Pro-Atherogenic Lipoprotein Profile in Pregnant Women with Maternal Supraphysiological Hypercholesterolemia
Authors: Cantin Claudette , Garchitorena María Jesús , Escalona Rodrigo , Carvajal Jorge A. , Illanes Sebastián E. , Gutierrez Jaime , Leiva Andrea
Published: April 2022

Maternal physiological hypercholesterolemia (MPH) occurs during pregnancy to assure fetal development. Some pregnant women develop maternal supraphysiological hypercholesterolemia (MSPH) characterized by increased levels of low-density lipoprotein (LDL). We aim to determine if proprotein convertase subtilisin/kexin type 9 (PCSK9) levels (a protein that regulate the availability of LDL receptor in the cells surface), as well as the composition and function of LDL, are modulated in MSPH women. This study included 122 pregnant women. Maternal total cholesterol (TC), LDL, triglycerides and PCSK9 increased from first (T1) to third trimester (T3) in MPH women. At T3, maternal TC, LDL, PCSK9 and placental abundances of PCSK9 were significantly higher in MPSH compared to MPH. Circulating PCSK9 levels were correlated with LDL at T3. In MSPH women, the levels of lipid peroxidation and oxidized LDL were significantly higher compared to MPH. LDL isolated from MSPH women presented significantly higher triglycerides and ApoB but lower levels of ApoAI compared to MPH. The formation of conjugated dienes was earlier in LDL from MSPH and in endothelial cells incubated with these LDLs; the levels of reactive oxygen species were significantly higher compared to LDL from MPH. We conclude that increased maternal PCSK9 would contribute to the maternal elevated levels of pro-atherogenic LDL in MSPH, which could eventually be related to maternal vascular dysfunction.
Effects of Stocking Density on Growth Performance, Antioxidant Status, and Meat Quality of Finisher Broiler Chickens under High Temperature
Authors: Son Jiseon , Kim Hee-Jin , Hong Eui-Chul , Kang Hwan-Ku
Published: April 2022

Environmental factors such as stocking density and high temperature can cause oxidative stress and negatively affect the physiological status and meat quality of broiler chickens. Here, we evaluated the effects of heat stress on the growth performance, antioxidant levels, and meat quality of broilers under different stocking densities. A total of 885 28-day-old male broilers (Ross 308) were subjected to five treatments (16, 18, 21, 23, and 26 birds/m2) and exposed to high temperatures (33 °C for 24 h) for 7 days. High stocking density (23 and 26 birds/m2) resulted in significantly decreased body weight (p < 0.01) and superoxide dismutase activity in the blood (p < 0.05) and increased (p < 0.05) rectal temperature and corticosterone. Additionally, the concentrations of heat shock protein 70 and malondialdehyde in the liver were higher in the 26 birds/m2 group (p < 0.05). Similarly, the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of breast meat increased linearly as the stocking density increased (p < 0.05). There was increased shear force in breast meat at low stocking density (p < 0.01). Thus, lower stocking density can relieve oxidative stress induced by high temperatures in broilers and improve the antioxidant capacity and quality of breast meat during hot seasons.
Passive Planar Microwave Devices
Authors: Martínez-Ros Alejandro Javier , Fernandez-Prieto Armando
Published: April 2022

Passive planar circuits play a key role in many RF/microwave applications, such as in wireless communications, medical instrumentation, and remote sensing [...]
Accurate Characterization of the Properties of the Rare-Earth-Doped Crystal for Laser Cooling
Authors: Duan Xuelu , Zhong Biao , Lei Yongqing , Wang Chaoyu , Xu Jiajin , Zhang Ziheng , Ding Jingxin , Yin Jianping
Published: April 2022

We present a method for calibrating a commercial thermal camera adopted to accurately measure the temperature change of the sample in a laser-induced temperature modulation spectrum (LITMoS) test, which is adopted for measuring two crucial parameters of the external quantum efficiency ηext and the background absorption coefficient αb for assessing the laser cooling grade of the rare-earth-doped materials. After calibration, the temperature resolution of the calibrated thermal camera is better than 0.1 K. For the cooling grade Czochralski-grown 5% Yb3+:LuLiF4 crystal, the corresponding values of ηext and αb are LITMoS = measured to be ηext=99.4 (±0.1)% and αb=1.5 (±0.1)×10−4 cm−1, respectively.
Development and Validation of Unmanned Aerial Vehicle Photogrammetry Simulator for Shaded Area Detection
Authors: Kim Jisung , Kim Jaekoo , Jeon Kyeongmi , Lee Joonmin , Lee Jaejoon
Published: April 2022

In unmanned aerial vehicle (UAV) photogrammetry, the qualities of three-dimensional (3D) models, including ground sample distance (GSD) and shaded areas, are strongly affected by flight planning. However, during flight planning, the quality of the output cannot be estimated, as it depends on the experience of the operator. Therefore, to reduce the time and cost incurred by repetitive work required to obtain satisfactory quality, a simulator, which can automatically generate a route, acquire images through simulation, and analyze the shaded areas without real flight, has been required. While some simulators have been developed, there are some limitations. Furthermore, evaluating the performance of the simulator is difficult owing to the lack of a validation method. Therefore, to overcome the limitations, target functions, which can plan flights and can detect shaded areas, were set, developed, and validated in this study. As a result, a simulator successfully planned a flight and detected shaded areas. In this way, the simulator was validated to determine the applicability of its performance. Furthermore, the outputs of this study can be applied to not only UAV photogrammetry simulators but also other 3D modeling simulators.