bluuebunny/crossref_metadata_2025 huggingface dataset:
Strengthening of Laminated Veneer Lumber Slabs with Fiber-Reinforced Polymer Sheets—Preliminary Study
Authors: Bakalarz Michał Marcin , Kossakowski Paweł Grzegorz
Published: February 2024

Analyzing the feasibility of reinforcing new and existing wooden structures is a valid problem, being the subject of numerous scientific papers. The paper presents the preliminary results of a study on reinforcing Laminated Veneer Lumber (LVL) panels with composite materials bonded to exterior surfaces using epoxy resin. Glass-Fiber-Reinforced Polymer (GFRP) sheets, Carbon-Fiber-Reinforced Polymer (CFRP) sheets, and Ultra-High-Modulus (UHM) CFRP sheets were used as reinforcement. The variables in the analysis were the type of reinforcement and the number of reinforcement layers. The tests were carried out on small samples (45 × 45 × 900 mm) subjected to the so-called four-point bending test. Reinforcement positively affected the mechanical properties of composite section. The highest increases in load bearing were 37 and 48% for two layers of GFRP and CFRP, respectively. The bending stiffness increased up to 53 and 62% for two layers of CFRP and UHM CFRP, respectively. There was a change in failure mode from cracking in the tension zone for unreinforced beams to veneer shear in the support zone (for CFRP and GFRP sheets) and sheet rupture (UHM CFRP). Good agreement was obtained for estimating bending stiffness with the presented numerical and mathematical model; the relative error was up to 6% for CFRP and GFRP and up to 20% for UHM CFRP. This preliminary study proved the effectiveness of combining LVL with FRP sheets and indicated their weak spots, which should be further analyzed to improve their competitiveness against the traditional structures. The key limitation was the shear strength of LVL.
Fire-Spread Characteristics and Evacuation Plan Optimization of Old Style Multi-Story Student Apartments
Authors: Wang Pu , Dai Hongtai , Yu Xiuhui , Wang Qingbiao , Li Shun , Jia Chuanyang
Published: February 2024

Fire is a major disaster event that can have a significant effect on public safety and social development. In a college or university, fire can seriously threaten the safety, lives, and property of those there due to the compact layout of apartment buildings and high population density. The ecological safety and sustainable development of buildings are also affected. In this study, PyroSim and Pathfinder software (version 2019) were used to simulate and analyze fire-spreading characteristics based on a multi-story university student apartment building. Additionally, the most effective safe evacuation plan from four fire evacuation drill schemes was identified by analyzing and comparing their performance. Results show that the spreading of fire smoke on different floors is significantly affected by the roof structure and the vertical and horizontal diffusion characteristics of smoke. While the smoke layer at the evacuation stairways has little effect on a safe evacuation, poor visibility due to smoke and ceiling temperatures has a significant effect. Safe evacuation becomes progressively more difficult at different floor levels from the top to the bottom of the building. The optimal safety scheme involves orderly evacuation through two open emergency exits. The number of emergency exits has a significant impact on the evacuation effectiveness. Measures and suggestions have been proposed to deal with apartment fires that address pre-event prevention, emergency loss reduction during the event, and post-event report-back. These proposals form an important theoretical reference for emergency evacuation and student apartment fire safety, providing important guidance for ecological safety protection of buildings and sustainable development.
In Vivo Monitoring of Glycerolipid Metabolism in Animal Nutrition Biomodel-Fed Smart-Farm Eggs
Authors: Zammit Victor A , Park Sang O
Published: February 2024

Although many studies have examined the biochemical metabolic pathways by which an egg (egg yolk) lowers blood lipid levels, data on the molecular biological mechanisms that regulate and induce the partitioning of hepatic glycerolipids are missing. The aim of this study was to investigate in vivo monitoring in four study groups using an animal nutrition biomodel fitted with a jugular-vein cannula after egg yolk intake: CON (control group, oral administration of 1.0 g of saline), T1 (oral administration of 1.0 g of pork belly fat), T2 (oral administration of 1.0 g of smart-farm egg yolk), and T3 (oral administration of T1 and T2 alternately every week). The eggs induced significant and reciprocal changes in incorporating 14C lipids into the total glycerolipids and releasing 14CO2, thereby regulating esterification and accelerating oxidation in vivo. The eggs increased phospholipid secretion from the liver into the blood and decreased triacylglycerol secretion by regulating the multiple cleavage of fatty acyl-CoA moieties’ fluxes. In conclusion, the results of the current study reveal the novel fact that eggs can lower blood lipids by lowering triacylglycerol secretion in the biochemical metabolic pathway of hepatic glycerolipid partitioning while simultaneously increasing phospholipid secretion and 14CO2 emission.
Probabilistic Risk Assessment of Metals, Acrylamide and Ochratoxin A in Instant Coffee from Brazil, Colombia, Mexico and Peru
Authors: Guadalupe Grobert A. , Grandez-Yoplac Dorila E. , Arellanos Erick , Doménech Eva
Published: February 2024

This study analysed the probabilistic risk to consumers associated with the presence of iAs, Cd, Cr, Hg, Pb, acrylamide (AA) and ochratoxin A (OTA) in instant coffee from Brazil, Colombia, Mexico and Peru. The results found iAs to be the metal with the highest concentrations (3.50 × 10−2 to 6.00 × 10−2 mg/kg), closely followed by Pb (1.70 × 10−2 to 2.70 × 10−2 mg/kg) and Cr (5.00 × 10−3 to 1.00 × 10−2 mg/kg), although these differences were not significant between countries. Cd and Hg were not detected. Focusing on AA, the concentrations ranged from 1.77 × 10−1 mg/kg (Peru) to 4.77 × 10−1 mg/kg (Brazil), while OTA ranged from 1.32 × 10−3 (Peru) to 1.77 × 10−3 mg/kg (Brazil) with significant differences between countries in both cases. As regards risk, the hazard quotient and hazard index were less than 1, meaning that the consumption of instant coffee represents a low level of concern for non-genotoxic effects. The results of the combination of margin of exposure and probability of exceedance indicated that the non-genotoxic effects of Pb, AA and OTA pose no threat. However, the probability values of suffering cancer from iAs and AA (between 1 × 10−6 and 1 × 10−4) indicated a moderate risk and that management measures should be taken.
Therapeutic Potential of Bacteroides fragilis SNBF-1 as a Next-Generation Probiotic: In Vitro Efficacy in Lipid and Carbohydrate Metabolism and Antioxidant Activity
Authors: Cang Weihe , Li Xuan , Tang Jiayi , Wang Ying , Mu Delun , Wu Chunting , Shi Haisu , Shi Lin , Wu Junrui , Wu Rina
Published: February 2024

This study explores the potential of aerotolerant Bacteroides fragilis (B. fragilis) strains as next-generation probiotics (NGPs), focusing on their adaptability in the gastrointestinal environment, safety profile, and probiotic functions. From 23 healthy infant fecal samples, we successfully isolated 56 beneficial B. fragilis strains. Notably, the SNBF-1 strain demonstrated superior cholesterol removal efficiency in HepG2 cells, outshining all other strains by achieving a remarkable reduction in cholesterol by 55.38 ± 2.26%. Comprehensive genotype and phenotype analyses were conducted, including sugar utilization and antibiotic sensitivity tests, leading to the development of an optimized growth medium for SNBF-1. SNBF-1 also demonstrated robust and consistent antioxidant activity, particularly in cell-free extracts, as evidenced by an average oxygen radical absorbance capacity value of 1.061 and a 2,2-diphenyl-1-picrylhydrazyl scavenging ability of 94.53 ± 7.31%. The regulation of carbohydrate metabolism by SNBF-1 was assessed in the insulin-resistant HepG2 cell line. In enzyme inhibition assays, SNBF-1 showed significant α-amylase and α-glucosidase inhibition, with rates of 87.04 ± 2.03% and 37.82 ± 1.36%, respectively. Furthermore, the cell-free supernatant (CFS) of SNBF-1 enhanced glucose consumption and glycogen synthesis in insulin-resistant HepG2 cells, indicating improved cellular energy metabolism. This was consistent with the observation that the CFS of SNBF-1 increased the proliferation of HepG2 cells by 123.77 ± 0.82% compared to that of the control. Overall, this research significantly enhances our understanding of NGPs and their potential therapeutic applications in modulating the gut microbiome.
Effects of the COVID-19 Pandemic on Dairy Consumption Trends: An Empirical Investigation of Accounting Data in China
Authors: Chen Jianxiong , Yang Chung-Cheng , Lin Yu
Published: February 2024

The COVID-19 pandemic may have had a negative impact on dairy consumption trends. Many dairy products are perishable and have relatively high income elasticity, causing their susceptibility to market fluctuations in general, including those specifically caused by the pandemic. However, the pandemic has also brought some other prospective possibilities. For example, during the pandemic, people paid more attention to nutrition and health issues and increased the number of meals prepared and eaten at home. In consideration of the particular circumstances during the pandemic, the Chinese government issued several policies to promote the population’s dairy consumption, and the Chinese dairy cattle sector actively implemented the policy of “guarantee price, quality, and supply”. These factors may have caused the Chinese population to increase their consumption of dairy products during the pandemic. Before the pandemic, the consumption of dairy products in the Chinese population showed an overall upward trend. The question addressed in this study is how has COVID-19 affected dairy consumption trends during the pandemic? This study uses accounting data from the Chinese dairy cattle sector to empirically analyze the impact of the COVID-19 pandemic on dairy consumption trends through economic theories and translog revenue function. Our study found that COVID-19 increased consumers’ consumption of dairy products in China, but those people experiencing poverty may still have experienced inadequate dairy intake. This study has contributed to the body of work in this area in the literature and provides response strategies for the dairy cattle sector and the authorities.
Preparation, Structural Identification, and Screening of Egg-Derived Peptides with Facilitating Alcohol Metabolism Activity
Authors: Tan Yali , Wang Yulin , Wan Yuan , Liang Yu , Liu Qiaocui , Wei Mengya , Hou Tao
Published: February 2024

The aim of this study was to obtain egg-derived peptides with facilitating alcohol metabolism (EPs) by enzymolysis, to identify their structures, and screen small polypeptides with higher activity by molecular docking. The optimum conditions for preparing EPs with facilitating alcohol metabolism were obtained by a single factor experiment, adding 2% Protamex and performing enzymolysis for 3 h with a liquid–material ratio of 35:1. The dose–response relationship experiment showed that 800 mg/kg·bw EPs played a better role in facilitating alcohol metabolism. EPs contained 40% hydrophobic amino acids (HAA), including 9.24% Leu. Eighty-four peptides were identified by HPLC-MS/MS and four peptides with potential activation of alcohol dehydrogenase were further selected by molecular docking. The tetrapeptide Trp-Ile-Val-Asp (WIVD) with the highest binding energy reached −7.16 kcal/mol. These findings suggest that egg is a good source for the preparation of peptides with facilitating alcohol metabolism activity.
Changes in Pore Structure and Fractal Characteristics of Solvents Pretreated High-Rank Coal under Supercritical CO2
Authors: Li Yong , Zhang Xiaodong , Sun Yijuan , Wang Zhen , Zhang Shuo , Li Binghui
Published: February 2024

CO2 injection in coal seams, which is a significant initiative to mitigate environmental problems caused by greenhouse gases, often leads a sequence of changes in the physical properties of coal reservoirs. To look into how the pore structure changes in the process of CO2 sequestration, we selected fresh coal from Huoerxinhe coal mine in China as the object. Then, acid treatment and SC-CO2 extraction were used to dissolve Organic and inorganic components in coal. Thus, by using SEM, LTGA-N2 apparatus and XRD, the characteristics of pore parameter and fractal dimension variation were discussed. The research results show that, the APS of samples THF, HCL-HF and Y-C increase, while the total PV decreases and the pore connectivity deteriorates. The pore connectivity of Samples THF and HCL-HF is improved (THF-C, HCL-HF-C), but the total pore volume continuously reduces. In addition, solvents treatment and SC-CO2 extraction mainly act on the microporous fraction. After solvents pretreatment, the changes in the pore size distribution curves are mainly manifested in the reduction of number of micropores, especially in the micropores around 3–4 nm. There is a small increase in micropores for samples Y-C and HCL-HF-C, with the pore size mainly concentrated around 4 nm, while the pores of the sample THF-C mainly show an increase within the scope of 3–16 nm. Generally, solvent pretreatment and SC-CO2 extraction help to simplify pore structure. However, the sample HCL-HF-C shows opposite change characteristics. In a short period of time, the larger pore fractal dimension, the less beneficial it is to the flow of CO2, while pore fractal dimension becomes progressively less useful in assessing pore connectivity with increasing time.
Target-Based 6-5 Fused Ring Heterocyclic Scaffolds Display Broad Antiparasitic Potency In Vitro
Authors: Dize Darline , Tali Mariscal Brice Tchatat , Ngansop Cyrille Armel Njanpa , Keumoe Rodrigue , Madiesse Kemgne Eugenie Aimée , Yamthe Lauve Rachel Tchokouaha , Tsouh Fokou Patrick Valere , Pone Kamdem Boniface , Hata Katsura , Fekam Boyom Fabrice
Published: February 2024

Malaria, leishmaniasis, and African trypanosomiasis are protozoan diseases that constitute major global health problems, especially in developing countries; however, the development of drug resistance coupled with the toxicity of current treatments has hindered their management. The involvement of certain enzymes (dihydrofolate reductase [DHFR]) or proteins (potassium channels) in the pathogenesis of these protozoan diseases is undeniable. In this study, a series of three DHFR inhibitors (6-5 fused heterocyclic derivatives X, Y, and Z) and one K+ channel blocker (E4031) were screened for their inhibitory effects on Leishmania donovani, Plasmodium falciparum, and Trypanosoma brucei. A resazurin assay was used to assess the antitrypanosomal and antileishmanial activities of the test compounds, whereas the antiplasmodial activity was evaluated through the SYBR Green I test. Moreover, the cytotoxicities of the test compounds were evaluated in Vero, Raw 264.7, and HepG-2 cells using a resazurin-based test, while their pharmacokinetic properties were predicted using the online tool, pkCSM. As a result, compound Y exhibited selective (selectivity index range: from 2.69 to >61.4; Vero, Raw 264.7, and HepG-2 cells) and broad-spectrum antiprotozoal activity against L. donovani promastigotes (IC50: 12.4 µM), amastigotes (IC50: 4.28 µM), P. falciparum (IC50: 0.028 µM), and T. brucei brucei (IC50: 0.81 µM). In addition, compound X inhibited the growth of P. falciparum (IC50: 0.0052 µM) and T. brucei brucei (IC50: 6.49 µM). In silico screening of the active antiprotozoal compounds revealed positive drug likeness scores, as none of the criteria for Lipinski’s rule were violated by these compounds. However, in-depth pharmacokinetic and mechanistic studies are warranted to support the discovery of novel antiprotozoal agents against malaria, leishmaniasis, and African trypanosomiasis by repurposing K+ channel blockers and DHFR inhibitors.
Rheological and Micro-Rheological Properties of Chicory Inulin Gels
Authors: Xu Jingyuan , Kenar James A.
Published: February 2024

As a soluble fiber, inulin is present in many plants and has many applications in food and non-food products. In this work, we investigated the rheological properties of inulin dispersions at seven concentrations. The linear viscoelastic properties of inulin were determined using a conventional mechanical rheometer. At 25 wt%, inulin exhibited fluid-like viscoelastic liquid behavior. However, when concentrations were ≥27.5 wt%, inulin exhibited gel-like viscoelastic properties. The viscoelastic properties (moduli and viscosities) increased with increasing inulin concentration. The high-frequency linear rheological properties of inulin were also investigated using the modern light scattering technique, diffusion wave spectroscopy (DWS). The diffusion wave spectroscopy (DWS) measurements showed the amplitude of complex moduli (|G*(ω)|) of inulin gels (≥27.5 wt%) to be proportional to ½ power law of the frequency, which suggests inulin gels behave similarly to flexible polymers. The non-linear steady shear experiments demonstrated that inulin exhibited shear-thinning behavior that was well fitted by a power law constitutive model. The trend of the power law exponent from the experiments indicated that the shear-thinning extent for inulin was greater as the inulin concentration increased. The results of this work indicated that the properties of inulin gel can be manipulated by altering its concentration. Therefore, the desired inulin product can be designed accordingly. These results can be used to direct further food and non-food applications, such as wound healing materials for inulin gels.
Stabilization of Shallow Landslides Induced by Rainwater Infiltration—A Case Study from Northern Croatia
Authors: Jug Jasmin , Grabar Kristijan , Bek Anja , Strelec Stjepan
Published: February 2024

Climate change brings with it phenomena such as large amounts of rainfall in short periods. Infiltration of rainwater into clayey soils is a common trigger for shallow landslides on slopes. In this way, numerous shallow landslides occur in the area of northern Croatia, and a characteristic example is the landslide “Orehovčak”. To stop the sliding of the destabilized slope, it was necessary to solve the drainage of water that infiltrates the landslide body. For this purpose, detailed geotechnical investigations and monitoring were conducted. Many data were collected at the investigation site, especially soil characteristics and groundwater fluctuations. The surface soil on the slope consists of highly plastic clay, and the sliding surface was created in contact with the solid subsoil of marl, the depth of which varies positionally. The analyses confirmed that water is a slip trigger. To solve the problem, excavations and installation of deep drains were performed. The slope safety factor confirms landslide stabilization, whose calculated value after rehabilitation was Fs = 1.645. Inclinometer readings carried out after remediation show that slope slippage stopped. This confirms that the presented remediation method is very applicable to shallow landslides in northern Croatia and similar landslides around the world.
Comment on Manzoor, H. et al. Efficacy of Different Dosing Regimens of IgE Targeted Biologic Omalizumab for Chronic Spontaneous Urticaria in Adult and Pediatric Populations: A Meta-Analysis. Healthcare 2022, 10, 2579
Authors: Tajerian Amin
Published: February 2024

In December 2022, the paper “Efficacy of Different Dosing Regimens of IgE Targeted Biologic Omalizumab for Chronic Spontaneous Urticaria in Adult and Pediatric Populations: A Meta-Analysis” was published in the Healthcare journal [...]
New Addictions in Late Adolescence and Emerging Adulthood: How Attachment Style May Predict Problematic Use of Social Networks and Binge-Watching
Authors: Tambelli Renata , Favieri Francesca , Casagrande Maria
Published: February 2024

As behavioral addictions (BAs) significantly affect well-being, paying attention to the characteristics associated with their onset is relevant. Current studies suggest that BAs should be addressed from an adaptive–maladaptive continuum perspective to define what and how some behaviors became problematic. The multi-faceted nature of behaviors attributed to possible BA involves psychological and individual backgrounds (e.g., attachment style). Given its role in affecting personality processes, social development, and motivational drives, the attachment style would be involved in addiction-like behaviors from adolescence, defined as a critical period for BA onset. This study analyzed the association between the attachment dimensions and two possible BAs that can be included in an adaptive–maladaptive continuum (i.e., social network use and TV series watching). A sample consisting of 493 late adolescents/emerging adults (age range: 18–24) completed questionnaires assessing social network use, TV series watching, and attachment style. The results showed a positive association between problematic attachment styles and BAs. High worry and need for relationships (anxious attachment) would be risk factors for problematic social network use and relationships as secondary (avoidant attachment) would be a risk factor for problematic TV series watching. These findings suggest the importance of further analyzing the role of attachment styles and their dimensions in influencing behavioral expression early to prevent the occurrence of BA.
Validity and Reliability of the Greek Version of Pittsburgh Sleep Quality Index in Chronic Non-Specific Low Back Pain Patients
Authors: Petropoulakos Kyriakos , Papakonstantinou Vasiliki , Pentsi Smaragda , Souzou Eftychia , Dimitriadis Zacharias , Billis Evdokia , Koumantakis Georgios , Poulis Ioannis , Spanos Savvas
Published: February 2024

The purpose of this study was to investigate psychometric properties of the Greek translation of Pittsburgh Sleep Quality Index (GR-PSQI) in a Greek chronic non-specific low back pain (CNSLBP) sample, thus, providing insight on its clarity and acceptability as a widely used sleep assessment tool in clinical practice. Asymptomatic volunteers (n = 73) and CNSLBP volunteers (n = 47), participated in the study. For the assessment of construct validity, the known-groups method was used. Thus, all the participants (asymptomatic and CNSLBP) completed the GR-PSQI. For the assessment of concurrent validity, the CNSLBP participants additionally completed the following validated questionnaires for depression, insomnia and sleep quality: Beck Depression Inventory Questionnaire (BDI), Insomnia Severity Index (ISI), and Sleep Quality Numeric Rating Scale (SQNRS). For the assessment of test–retest reliability, the CNSLBP participants completed the GR-PSQI a second time, one week after the first time. The results showed excellent test–retest reliability (ICC = 0.969, SEM = 0.90, SDD = 2.49%) and internal consistency (Cronbach α = 0.985), moderate to good concurrent validity (from r = 0.556 to r = 0.860) among PSQI, BDI, SQNRS, and ISI, as well as excellent construct validity (p = 0.000) between the two groups. The Greek translation of PSQI could be a valuable tool for Greek healthcare professionals in both clinical and research environments.
Perceptions, Beliefs, and Experiences about the Menstrual Cycle and Menstruation among Young Women: A Qualitative Approach
Authors: Botello-Hermosa Alicia , González-Cano-Caballero María , Guerra-Martín María Dolores , Navarro-Pérez Carmen Flores , Arnedillo-Sánchez Socorro
Published: February 2024

The experience of menstruation is often associated with negative connotations and gender stereotypes, which results in making it invisible. This research aimed to explore the perceptions, beliefs, and knowledge of young Spanish women regarding the menstrual cycle and menstruation and their impact on their lives. The study delves into their understanding, menstrual management practices, the types of menstrual products employed, and their experiences related to menstrual health. Qualitative methodology was used with discussion groups as a data collection technique. The participants comprised 45 young Spanish women, aged between 18 and 23, hailing from both rural and urban areas. The majority were university students, with some engaged in part-time work, and one participant working full-time. While many experienced menstrual pain ranging from mild to debilitating, a normalization of this pain often led them to forego seeking specialist assistance. Disposable menstrual products (DMPs) are the most used by participants, despite limited awareness of their absorption capacity. Regarding reusable menstrual products (RMPs), menstrual cup users emphasized comfort but expressed a need for proper training. Negative menstruation experiences could evoke fear and difficulties, underscoring the importance of providing comprehensive menstrual health education encompassing both theoretical and practical components.
RETRACTED: Ai et al. The Effects of Environmental Regulations on Medical Expenses: Evidence from China. Int. J. Environ. Res. Public Health 2022, 19, 7567
Authors: Ai Hongshan , Tan Xiaoqing , Xia Zhen
Published: February 2024

The authors and journal retract the following article: “The Effects of Environmental Regulations on Medical Expenses: Evidence from China” [...]
Spatial Accessibility to Primary Care in Metropolitan France: Results Using the SCALE Spatial Accessibility Index for All Regions
Authors: Launay Ludivine , Guillot Fabien , Medjkane Mohand , Launoy Guy , Dejardin Olivier
Published: February 2024

Accessibility to care is a major public health issue. Various tools to assess it are available, but they do not solve the problem of scale. Moreover, accessibility is a multidimensional concept that is not taken into account with current tools. The SCALE index aims to overcome these two limitations by proposing a synthetic measure on a more precise scale than the administrative unit or the sub-municipal scale. Under the assumption of access to care facilities for all and access to the nearest facilities, the potential accessibility distance was calculated for each couple (residential area, accessible facilities). This was defined as the average distance by road that the population has to travel to access care. To take the availability of resources into account, these distances were weighted by the theoretical pressure on the facilities. The SCALE index was then calculated using a linear combination of the distances of potential accessibility to care facilities It highlights differences in accessibility at the national and regional scale. Using this index, it was possible to provide maps for all French regions and the major cities in a story-map. The major conurbation around Paris and the main urban centers has high accessibility. Low accessibility forms a “Y” shape. In conclusion, the SCALE index measures accessibility at the scale of a small geographic unit taking the proximity and the availability of health professionals into account. It is also possible to take into account the diversity of accessibility in a given territory.
Enhanced Seamless Indoor–Outdoor Tracking Using Time Series of GNSS Positioning Errors
Authors: Angelats Eduard , Gorreja Alban , Espín-López Pedro F. , Parés M. Eulàlia , Malinverni Eva Savina , Pierdicca Roberto
Published: February 2024

The seamless integration of indoor and outdoor positioning has gained considerable attention due to its practical implications in various fields. This paper presents an innovative approach aimed at detecting and delineating outdoor, indoor, and transition areas using a time series analysis of Global Navigation Satellite System (GNSS) error statistics. By leveraging this contextual understanding, the decision-making process between GNSS-based and Visual-Inertial Odometry (VIO) for trajectory estimation is refined, enabling a more robust and accurate positioning. The methodology involves three key steps: proposing the division of our context environment into a set of areas (indoor, outdoor, and transition), exploring two methodologies for the classification of space based on a time series of GNSS error statistics, and refining the trajectory estimation strategy based on contextual knowledge. Real data across diverse scenarios validate the approach, yielding trajectory estimations with accuracy consistently below 10 m.
Bayesian Shared Component Spatial Modeling for Assessing the Shared and Age Group-Specific Mental Health Disorder Risk of Young and Old Age Groups: A Case Study of Toronto Neighborhoods, Canada
Authors: Abdullah Abu Yousuf Md , Law Jane
Published: February 2024

Mental health disorder risks of young and old age groups hold considerable importance for understanding present and future risk burdens. However, assessing mental health risks is significantly constrained by the influence of shared and age group-specific spatial processes and risk factors. Therefore, this study employed Bayesian shared component spatial modeling (BSCSM) to analyze mental health disorder data obtained from young (20–44 years) and old (65+ years) age groups in Toronto. BSCSM was employed to model the shared and age group-specific disorder risk and to identify hotspot areas. The unmeasured covariates, overdispersion, and latent spatial processes were adjusted using spatial and non-spatial random effect terms. The findings from BSCSM were finally compared with non-shared component modeling approaches. The results suggest that over 60% of variations in mental health disorder risk for both age groups could be explained by the shared component. The high-risk neighborhoods were mainly localized in southern and north-central Toronto for the young and old age groups. Deviance information criterion values suggested that models from BSCSM outperformed non-BSCSM models. BSCSM risk maps were also better at identifying high-risk areas. This work demonstrated that both shared and age group-specific risks are essential for assessing mental health disorder risk and devising targeted interventions.
ER Stress-Activated HSF1 Governs Cancer Cell Resistance to USP7 Inhibitor-Based Chemotherapy through the PERK Pathway
Authors: Lim Chang-Hoon , Fang Xue-Quan , Kang Hyeji , Oh Taerim , Lee Seonghoon , Kim Young-Seon , Lim Ji-Hong
Published: February 2024

Ubiquitin-specific protease 7 inhibitors (USP7i) are considered a novel class of anticancer drugs. Cancer cells occasionally become insensitive to anticancer drugs, known as chemoresistance, by acquiring multidrug resistance, resulting in poor clinical outcomes in patients with cancer. However, the chemoresistance of cancer cells to USP7i (P22077 and P5091) and mechanisms to overcome it have not yet been investigated. In the present study, we generated human cancer cells with acquired resistance to USP7i-induced cell death. Gene expression profiling showed that heat stress response (HSR)- and unfolded protein response (UPR)-related genes were largely upregulated in USP7i-resistant cancer cells. Biochemical studies showed that USP7i induced the phosphorylation and activation of heat shock transcription factor 1 (HSF1), mediated by the endoplasmic reticulum (ER) stress protein kinase R-like ER kinase (PERK) signaling pathway. Inhibition of HSF1 and PERK significantly sensitized cancer cells to USP7i-induced cytotoxicity. Our study demonstrated that the ER stress–PERK axis is responsible for chemoresistance to USP7i, and inhibiting PERK is a potential strategy for improving the anticancer efficacy of USP7i.
Transcriptomic Signatures of Neuronally Derived Extracellular Vesicles Reveal the Presence of Olfactory Receptors in Clinical Samples from Traumatic Brain Injury Patients
Authors: Bhomia Manish , Feng Yanru , Deleon Piper , Robertson Claudia S. , Kobeissy Firas , Wang Kevin K. , Knollmann-Ritschel Barbara
Published: February 2024

Traumatic brain injury (TBI) is defined as an injury to the brain by external forces which can lead to cellular damage and the disruption of normal central nervous system functions. The recently approved blood-based biomarkers GFAP and UCH-L1 can only detect injuries which are detectable on CT, and are not sensitive enough to diagnose milder injuries or concussion. Exosomes are small microvesicles which are released from the cell as a part of extracellular communication in normal as well as diseased states. The objective of this study was to identify the messenger RNA content of the exosomes released by injured neurons to identify new potential blood-based biomarkers for TBI. Human severe traumatic brain injury samples were used for this study. RNA was isolated from neuronal exosomes and total transcriptomic sequencing was performed. RNA sequencing data from neuronal exosomes isolated from serum showed mRNA transcripts of several neuronal genes. In particular, mRNAs of several olfactory receptor genes were present at elevated concentrations in the neuronal exosomes. Some of these genes were OR10A6, OR14A2, OR6F1, OR1B1, and OR1L1. RNA sequencing data from exosomes isolated from CSF showed a similar elevation of these olfactory receptors. We further validated the expression of these samples in serum samples of mild TBI patients, and a similar up-regulation of these olfactory receptors was observed. The data from these experiments suggest that damage to the neurons in the olfactory neuroepithelium as well as in the brain following a TBI may cause the release of mRNA from these receptors in the exosomes. Hence, olfactory receptors can be further explored as biomarkers for the diagnosis of TBI.
Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance
Authors: Slepičková Kasálková Nikola , Juřicová Veronika , Fajstavr Dominik , Frýdlová Bára , Rimpelová Silvie , Švorčík Václav , Slepička Petr
Published: February 2024

We focused on polydimethylsiloxane (PDMS) as a substrate for replication, micropatterning, and construction of biologically active surfaces. The novelty of this study is based on the combination of the argon plasma exposure of a micropatterned PDMS scaffold, where the plasma served as a strong tool for subsequent grafting of collagen coatings and their application as cell growth scaffolds, where the standard was significantly exceeded. As part of the scaffold design, templates with a patterned microstructure of different dimensions (50 × 50, 50 × 20, and 30 × 30 μm2) were created by photolithography followed by pattern replication on a PDMS polymer substrate. Subsequently, the prepared microstructured PDMS replicas were coated with a type I collagen layer. The sample preparation was followed by the characterization of material surface properties using various analytical techniques, including scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). To evaluate the biocompatibility of the produced samples, we conducted studies on the interactions between selected polymer replicas and micro- and nanostructures and mammalian cells. Specifically, we utilized mouse myoblasts (C2C12), and our results demonstrate that we achieved excellent cell alignment in conjunction with the development of a cytocompatible surface. Consequently, the outcomes of this research contribute to an enhanced comprehension of surface properties and interactions between structured polymers and mammalian cells. The use of periodic microstructures has the potential to advance the creation of novel materials and scaffolds in tissue engineering. These materials exhibit exceptional biocompatibility and possess the capacity to promote cell adhesion and growth.
Is Silver Addition to Scaffolds Based on Polycaprolactone Blended with Calcium Phosphates Able to Inhibit Candida albicans and Candida auris Adhesion and Biofilm Formation?
Authors: Menotti Francesca , Scutera Sara , Maniscalco Eleonora , Coppola Bartolomeo , Bondi Alessandro , Costa Cristina , Longo Fabio , Mandras Narcisa , Pagano Claudia , Cavallo Lorenza , Banche Giuliana , Malandrino Mery , Palmero Paola , Allizond Valeria
Published: February 2024

Candida spp. periprosthetic joint infections are rare but difficult-to-treat events, with a slow onset, unspecific symptoms or signs, and a significant relapse risk. Treatment with antifungals meets with little success, whereas prosthesis removal improves the outcome. In fact, Candida spp. adhere to orthopedic devices and grow forming biofilms that contribute to the persistence of this infection and relapse, and there is insufficient evidence that the use of antifungals has additional benefits for anti-biofilm activity. To date, studies on the direct antifungal activity of silver against Candida spp. are still scanty. Additionally, polycaprolactone (PCL), either pure or blended with calcium phosphate, could be a good candidate for the design of 3D scaffolds as engineered bone graft substitutes. Thus, the present research aimed to assess the antifungal and anti-biofilm activity of PCL-based constructs by the addition of antimicrobials, for instance, silver, against C. albicans and C. auris. The appearance of an inhibition halo around silver-functionalized PCL scaffolds for both C. albicans and C. auris was revealed, and a significant decrease in both adherent and planktonic yeasts further demonstrated the release of Ag+ from the 3D constructs. Due to the combined antifungal, osteoproliferative, and biodegradable properties, PCL-based 3D scaffolds enriched with silver showed good potential for bone tissue engineering and offer a promising strategy as an ideal anti-adhesive and anti-biofilm tool for the reduction in prosthetic joints of infections caused by Candida spp. by using antimicrobial molecule-targeted delivery.
MOGAT: A Multi-Omics Integration Framework Using Graph Attention Networks for Cancer Subtype Prediction
Authors: Tanvir Raihanul Bari , Islam Md Mezbahul , Sobhan Masrur , Luo Dongsheng , Mondal Ananda Mohan
Published: February 2024

Accurate cancer subtype prediction is crucial for personalized medicine. Integrating multi-omics data represents a viable approach to comprehending the intricate pathophysiology of complex diseases like cancer. Conventional machine learning techniques are not ideal for analyzing the complex interrelationships among different categories of omics data. Numerous models have been suggested using graph-based learning to uncover veiled representations and network formations unique to distinct types of omics data to heighten predictions regarding cancers and characterize patients’ profiles, amongst other applications aimed at improving disease management in medical research. The existing graph-based state-of-the-art multi-omics integration approaches for cancer subtype prediction, MOGONET, and SUPREME, use a graph convolutional network (GCN), which fails to consider the level of importance of neighboring nodes on a particular node. To address this gap, we hypothesize that paying attention to each neighbor or providing appropriate weights to neighbors based on their importance might improve the cancer subtype prediction. The natural choice to determine the importance of each neighbor of a node in a graph is to explore the graph attention network (GAT). Here, we propose MOGAT, a novel multi-omics integration approach, leveraging GAT models that incorporate graph-based learning with an attention mechanism. MOGAT utilizes a multi-head attention mechanism to extract appropriate information for a specific sample by assigning unique attention coefficients to neighboring samples. Based on our knowledge, our group is the first to explore GAT in multi-omics integration for cancer subtype prediction. To evaluate the performance of MOGAT in predicting cancer subtypes, we explored two sets of breast cancer data from TCGA and METABRIC. Our proposed approach, MOGAT, outperforms MOGONET by 32% to 46% and SUPREME by 2% to 16% in cancer subtype prediction in different scenarios, supporting our hypothesis. Our results also showed that GAT embeddings provide a better prognosis in differentiating the high-risk group from the low-risk group than raw features.
Dynamics of Chromatin Opening across Larval Development in the Urochordate Ascidian Ciona savignyi
Authors: He Muchun , Li Yuting , Li Yajuan , Dong Bo , Yu Haiyan
Published: February 2024

Ascidian larvae undergo tail elongation and notochord lumenogenesis, making them an ideal model for investigating tissue morphogenesis in embryogenesis. The cellular and mechanical mechanisms of these processes have been studied; however, the underlying molecular regulatory mechanism remains to be elucidated. In this study, assays for transposase-accessible chromatin using sequencing (ATAC-seq) and RNA sequencing (RNA-seq) were applied to investigate potential regulators of the development of ascidian Ciona savignyi larvae. Our results revealed 351 and 138 differentially accessible region genes through comparisons of ATAC-seq data between stages 21 and 24 and between stages 24 and 25, respectively. A joint analysis of RNA-seq and ATAC-seq data revealed a correlation between chromatin accessibility and gene transcription. We further verified the tissue expression patterns of 12 different genes. Among them, Cs-matrix metalloproteinase 24 (MMP24) and Cs-krüppel-like factor 5 (KLF5) were highly expressed in notochord cells. Functional assay results demonstrated that both genes are necessary for notochord lumen formation and expansion. Finally, we performed motif enrichment analysis of the differentially accessible regions in different tailbud stages and summarized the potential roles of these motif-bearing transcription factors in larval development. Overall, our study found a correlation between gene expression and chromatin accessibility and provided a vital resource for understanding the mechanisms of the development of ascidian embryos.
Genetic Loci of Plant Pathogenic Dickeya solani IPO 2222 Expressed in Contact with Weed-Host Bittersweet Nightshade (Solanum dulcamara L.) Plants
Authors: Czajkowski Robert , Krzyżanowska Dorota M. , Sokolova Daryna , Rąbalski Łukasz , Kosiński Maciej , Jafra Sylwia , Królicka Aleksandra
Published: February 2024

Dickeya solani, belonging to the Soft Rot Pectobacteriaceae, are aggressive necrotrophs, exhibiting both a wide geographic distribution and a wide host range that includes many angiosperm orders, both dicot and monocot plants, cultivated under all climatic conditions. Little is known about the infection strategies D. solani employs to infect hosts other than potato (Solanum tuberosum L.). Our earlier study identified D. solani Tn5 mutants induced exclusively by the presence of the weed host S. dulcamara. The current study assessed the identity and virulence contribution of the selected genes mutated by the Tn5 insertions and induced by the presence of S. dulcamara. These genes encode proteins with functions linked to polyketide antibiotics and polysaccharide synthesis, membrane transport, stress response, and sugar and amino acid metabolism. Eight of these genes, encoding UvrY (GacA), tRNA guanosine transglycosylase Tgt, LPS-related WbeA, capsular biosynthesis protein VpsM, DltB alanine export protein, glycosyltransferase, putative transcription regulator YheO/PAS domain-containing protein, and a hypothetical protein, were required for virulence on S. dulcamara plants. The implications of D. solani interaction with a weed host, S. dulcamara, are discussed.
An In Vitro Study Evaluating the Safety of Mesalazine on Human Nasoepithelial Cells
Authors: Murphy William , Liu Sha , Javadiyan Shari , Vyskocil Erich , Feizi Sholeh , Callejas Claudio , Wormald Peter-John , Vreugde Sarah , Psaltis Alkis J.
Published: February 2024

Chronic rhinosinusitis (CRS) is a disease characterised by the inflammation of the nasal and paranasal cavities. It is a widespread condition with considerable morbidity for patients. Current treatment for chronic rhinosinusitis consists of appropriate medical therapy followed by surgery in medically resistant patients. Although oral steroids are effective, they are associated with significant morbidity, and disease recurrence is common when discontinued. The development of additional steroid sparing therapies is therefore needed. Mesalazine is a commonly used therapeutic in inflammatory bowel disease, which shares a similar disease profile with chronic rhinosinusitis. This exploratory in vitro study aims to investigate whether mesalazine could be repurposed to a nasal wash, which is safe on human nasoepithelial cells, and retains its anti-inflammatory effects. CRS patients’ human nasal epithelial cells (HNECs) were collected. HNECs were grown at an air-liquid interface (ALIs) and in a monolayer and challenged with mesalazine or a non-medicated control. Transepithelial electrical resistance, paracellular permeability, and toxicity were measured to assess epithelial integrity and safety. The anti-inflammatory effects of mesalazine on the release of interleukin (IL)-6 and tumour necrosis factor alpha (TNF-α) were analysed using human leukemia monocytic cell line (THP-1). mesalazine did not impact the barrier function of HNEC-ALIs and was not toxic when applied to HNECs or THP-1 cells at concentrations up to 20 mM. mesalazine at 0.5 and 1 mM concentrations significantly inhibited TNF-α release by THP-1 cells. mesalazine effectively decreases TNF-α secretion from THP-1 cells, indicating the possibility of its anti-inflammatory properties. The safety profile of mesalazine at doses up to 20 mM suggests that it is safe when applied topically on HNECs.
Human Papilloma Virus Positive Oropharyngeal Squamous Cell Carcinoma and the Immune System: Pathogenesis, Immunotherapy and Future Perspectives
Authors: Khoo A. , Boyer M. , Jafri Z. , Makeham T. , Pham T. , Khachigian L. M. , Floros P. , Dowling E. , Fedder K. , Shonka D. , Garneau J. , O’Meara C. H.
Published: February 2024

Oropharyngeal squamous cell carcinoma (OPSCC), a subset of head and neck squamous cell carcinoma (HNSCC), involves the palatine tonsils, soft palate, base of tongue, and uvula, with the ability to spread to adjacent subsites. Personalized treatment strategies for Human Papillomavirus-associated squamous cell carcinoma of the oropharynx (HPV+OPSCC) are yet to be established. In this article, we summarise our current understanding of the pathogenesis of HPV+OPSCC, the intrinsic role of the immune system, current ICI clinical trials, and the potential role of small molecule immunotherapy in HPV+OPSCC.
Regulation of Disease-Resistance Genes against CWMV Infection by NbHAG1-Mediated H3K36ac
Authors: Tu Aizhu , Wu Mila , Jiang Yaoyao , Guo Lidan , Guo Yunfei , Wang Jinnan , Xu Gecheng , Shi Jingjing , Chen Jianping , Yang Jian , Zhong Kaili
Published: February 2024

Post-translational modification of proteins plays a critical role in plant–pathogen interactions. Here, we demonstrate in Nicotiana benthamiana that knockout of NbHAG1 promotes Chinese wheat mosaic virus (CWMV) infection, whereas NbHAG1 overexpression inhibits infection. Transcriptome sequencing indicated that a series of disease resistance-related genes were up-regulated after overexpression of NbHAG1. In addition, cleavage under targets and tagmentation (Cut&Tag)-qPCR results demonstrated that NbHAG1 may activate the transcription of its downstream disease-resistance genes by facilitating the acetylation level of H3K36ac. Therefore, we suggest that NbHAG1 is an important positive regulator of resistance to CWMV infestation.