bluuebunny/crossref_metadata_2025 huggingface dataset:
Alterações emocionais em estudantes concluintes do curso de Fisioterapia de uma instituição pública de ensino superior
Authors: Silva Bárbara Leite da , Souza Ana Paula de Carvalho , Fortaleza Lílian Melo de Miranda
Published: September 2023

Objetivo: Identificar as alterações emocionais em estudantes concluintes do curso de Fisioterapia de uma instituição pública de ensino superior. Metodologia: Estudo transversal, observacional e quantitativo descritivo, realizado com estudantes de Fisioterapia de uma instituição pública de ensino superior localizada em Teresina - PI. Foram utilizados um questionário sociodemográfico e a Escala de Depressão, Ansiedade e Estresse (DASS 21). Participaram do estudo estudantes regularmente matriculados no 9º e 10º período da graduação, com idade igual ou superior a 18 anos e de ambos os sexos. Para processamento e análise dos dados foi utilizado o programa IBM Statistical Package for the Social Sciences versão 20.0. Resultados: A amostra foi composta por 30 estudantes, sendo a maioria do sexo feminino (86,7%) e idade média de 23,83 ± 1,34. Encontrou-se uma prevalência significativa de sintomas de ansiedade (70,1%), estresse (86,6%) e depressão (73,3%). Sendo os sintomas de estresse e depressão, as alterações emocionais mais prevalentes, indicando 13 (43,3%) na fase moderada e 13 (43,3%) na fase severa, respectivamente. Houve diferença significativa no aspecto ansiedade com as variáveis estado civil e a prática de exercícios físicos, bem como no estresse com a variável raça. Conclusão: Os estudantes concluintes de Fisioterapia apresentaram maior prevalência dos sintomas de estresse e depressão. Espera-se que os resultados possam contribuir para a projeção de estratégias de identificação precoce e intervenções levando à promoção de saúde mental para este público.
Desafios na assistência ao paciente com tuberculose na atenção básica
Authors: Silva Vitória Régia Vieira da , Mantey Jacques Magnos Canossa , Oliveira Joyce Josefa de , Silva Erika de Nazare Rodrigues da , Souza Carolayne Pereira de , Araújo Vanusa Maria da Silva , Caldas Natália da Silva , Otaviano Kaio Rodrigues , Duarte Letícia Barbosa , Ferreira Yasmim Alves , Oliveira Thays Flávia Silva
Published: September 2023

A tuberculose (TB) é uma doença infectocontagiosa, com altas taxas de incidência e prevalência, e encontra-se associada a baixas condições socioeconômicas. Nesse cenário, a Atenção Primária a Saúde (APS), como coordenadora do cuidado, deve ser capaz de promover ações integrais, visando melhorar a qualidade de vida e controlar efetivamente a doença. No entanto, ainda existem diversos desafios na assistência a esses pacientes. Diante disso, esse estudo tem como objetivo identificar os desafios na assistência de enfermagem ao paciente com tuberculose na atenção básica. Trata-se de uma revisão integrativa da literatura, realizado em julho e agosto de 2023, através dos Descritores em Ciências da Saúde (DeCS): “Tuberculose”, “Atenção primária” e “Enfermagem”. Foram encontrados 127 artigos e, destes após uma seleção criteriosa, foram utilizados 12 artigos para compor essa pesquisa. A partir dos estudos encontrados, evidencia-se que os principais desafios na assistência ao paciente com TB envolvem a falta de conhecimento, falta de acesso, baixas condições financeiras, falta de acessibilidade, ausência e alta rotatividade dos profissionais, ausência de incentivos e comportamentos estigmatizantes. Diante disso, torna-se importante que o cuidado ao paciente com tuberculose envolva o usuário, profissionais, a família, a gestão dos serviços de saúde e seja articulada com outros setores, de modo que suas necessidades sejam resolutivas, e que tais desafios sejam superados.
Avaliação e conduta bucomaxilofacial em casos de fraturas do côndilo mandibular
Authors: Gomes Luize Marinho , Costa Tarsila Eshyla Silva , Nascimento Wendy Saureana Maior de Oliveira , Lima Isabella Ferreira , Albuquerque Isabelle Cristhina Machado de , Gomes Joice Martins , Lima Thalyta Estefani Gomes da Silva
Published: September 2023

As fraturas condilares apresentam uma incidência significativa dentre as fraturas faciais. O côndilo faz parte da estrutura da articulação temporomandibular (ATM), que é responsável pelos movimentos mandibulares, mastigação e fonação. Logo, em casos de fraturas, é necessário que o profissional faça uma avaliação adequada através de achados clínicos e exames de imagem, como a tomografia computadorizada, que permite verificar a localização e extensão da fratura. Com isso, será possível realizar um correto diagnóstico e plano de tratamento. Nesse sentido, o presente trabalho tem como objetivo abordar os métodos de avaliação e os princípios de tratamento das fraturas do côndilo mandibular, de modo a se ter um prognóstico favorável. Para desenvolver o presente artigo, pesquisas foram feitas nas bases de dados Google Acadêmico e SciELO a fim de revisar a literatura, com obras de 2012 a 2022 na língua portuguesa e inglesa. Esta revisão permitiu conferir que uma avaliação detalhada da fratura é fundamental para a escolha da conduta terapêutica. Além disso, pôde-se concluir que tanto o tratamento conservador como o cirúrgico são efetivos quando indicados de forma correta pelo cirurgião.
Sistema de Gestão da Qualidade: Uma abordagem à produção eficiente
Authors: Soares Wellington Souza , Câmara Rafaela Almeida , Souza Wagner Cavalare de
Published: September 2023

O Sistema de Gestão da Qualidade – SGQ, traz por meios de aplicação e de ferramentas, a padronização de processos que entregam como um dos resultados, o valor de agregação ao cliente. Os processos de implantação e conformação da qualidade da empresa, é constituído de processos de médio/longo prazo que devem ser acompanhados constantemente e aplicando melhorias contínuas. Este estudo tem como objetivo, realizar um levantamento bibliográfico com foco nas ferramentas da gestão da qualidade à produção eficiente com aplicação em diversos setores da indústria. Assim, toma-se como base estudos de caso disponíveis nas bibliografias dos últimos 16 anos, bem como detalhar ferramentas a fim de auxiliar nas definições das metas e indicadores para seus processos, apresentando às lideranças a importância da implantação e/ou aperfeiçoamento do programa da qualidade dentro do empreendimento, onde tem como resultados positivos e satisfatórios usando as ferramentas de gestão de qualidade.
Análise comparativa do comportamento mecânico do solo típico de Manaus/AM estabilizado granulometricamente com resíduo de tijolo
Authors: Silva Vanessa Melo da , Tello Ramírez Bruno , Silva Paulo Sérgio da , Pereira Alex Gomes , Lima João Henrique Lacerda Melo , Silva Matheus Pena da Silva e
Published: September 2023

No presente trabalho pesquisou-se o aproveitamento do resíduo de tijolos cerâmicos provenientes de olarias do município de Iranduba/Amazonas (AM), para fins de obtenção da estabilização mecânica do solo típico de Manaus/AM, além de propor um descarte adequado do material. O objetivo principal foi estudar o comportamento mecânico com efeito da adição do resíduo ao solo natural, no qual se configura extremamente argiloso, melhorados com adições de 5, 10 e 15% de resíduo. A caracterização do solo natural realizou-se por realizar os ensaios de massa específica dos grãos, granulometria, limites de Atterberg, Proctor na energia intermediária e as misturas se sucederam por ensaios limites de Atterberg e Proctor na energia intermediária. Os resíduos, por sua vez, foram moídos no equipamento do Ensaio de Abrasão Los Angeles e analisaram-se quanto à sua textura, pelo ensaio de granulometria, e massa específica dos grãos. Referente ao comportamento mecânico, tanto o solo natural quanto às composições foi avaliado por meio do ensaio de cisalhamento direto. Por meio dos resultados, notou-se que o resíduo cerâmico trouxe melhoras no comportamento mecânico do solo, onde a adição do resíduo cerâmico reduziu a coesão e aumentou o ângulo de atrito.
IN SILICO EVALUATION OF SARS-COV-2 PAPAIN-LIKE PROTEASE INHIBITORY ACTIVITY OF SOME FDA-APPROVED DRUGS
Authors: EROL Meryem
Published: September 2023

Objective: In this study, it was aimed to perform in silico studies on the papain-like protease structure of SARS-CoV-2 (PDB: 7JIT) of 1300 FDA-approved drugs downloaded from the ZINC database.
Material and Method: A molecular docking study was performed with PLpro (PDB ID: 7JIT) using four different molecular docking programs for a total of 1300 FDA-approved drugs obtained from the ZINC database. Conivaptan and amphotericin B were obtained in docking analysis with AutoDock Vina and Sybyl-X, respectively. Docking analysis with Glide SP and Glide XP resulted in fludarabine and panobinostat, respectively. Molecular dynamics simulations were performed for a period of 120 ns to check the stability of these four drugs.
Result and Discussion: The reliability of the results obtained using four different molecular docking programs on the SARS-CoV-2 papain-like protease of 1300 drug molecules was checked by reinserting the co-crystal ligand. Protein-ligand interactions between fludarabine, conivaptan, amphotericin-B, panobinostat, and PLpro were given. In the molecular dynamics study, RMSD, RMSF, Rg, and SASA analyses were performed for four systems. It was observed that RMSD remained constant for all 120 ns for all four systems except for amphotericin B, which deviated slightly towards the end of 120 ns. No significant fluctuation was noticed in the RMSF graphics for all four systems.
Pelatihan Penggunaan Website Desa Indrapura
Authors: Effendy Irman , Bakti Ahmad Mutatkin , Syazili Ahmad , Mirza A. Haidar , Ariandi Muhamad , Santoso Arif Budi
Published: September 2023

Pengabdian masyarakat merupakan salah satu pilar dari tri dharma perguruan yang bertujuan membantu masyarakat dengan menerapkan ilmu pengetahuan yang dimilki atau hasil dari penelitian yang dilakukan. Pengabdian masyarakat ini bertujuan memberikan pelatihan kepada perangkat Desa Indrapura dalam memanfaatkan sebuah website desa. Dengan pelatihan ini maka akan membantu kepala desa untuk mempromosikan potensi desa, berita dan pengumuman khususnya kepada warga desa tersebut. Dalam pengembangan dan perancangan website desa, penulis menggunakan metode waterfall. Analisis data dilakukan dengan teknik kualitatif, untuk pengumpulan data dengan melakukan wawancara secara langsung kepada salah satu pengurus di desa Indrapura dan telah melakukan observasi ketempat objek tersebut untuk mendapatkan data yang valid. Website desa yang dihasilkan akan diserahkan kepada Desa Indrapura sebagai bentuk pengabdian peneliti untuk memenuhi satu tri dharma perguruan tinggi.
Distrusting Consensus: How a Uniform Corona Pandemic Narrative Fostered Suspicion and Conspiracy Theories
Authors: Harambam Jaron
Published: September 2023

Although the institutional model of science communication operated well during the corona-pandemic, and relevant public institutions (media, science, politics) garnered higher levels of trust following “rally-around-the-flag” dynamics, other people would develop distrusts towards those institutions and the emerging orthodox corona narrative. Their ideas are often framed as conspiracy theories, and today’s globalized media eco-system enables their proliferation. This looming “infodemic” became a prime object of concern. In this article I agnostically study those distrusts from a cultural sociological perspective to better understand how and why people (came to) disbelieve official knowledge and their producers. To do so, I draw on my ethnographic fieldwork in the off- and online worlds of people labeled as conspiracy theorists in the Netherlands, which includes the media they consume, share and produce. Based on an inductive analysis of people’s own sense-making, I present three dominant reasons: media’s panicky narrative of fear and mayhem; governments sole focus on lockdowns and vaccines; and the exclusion of heterodox scientific perspectives in the public sphere. Each of these reasons problematize a perceived orthodoxy in media, politics and science, and this uniformity bred suspicion about possible conspiracies between these public institutions. Too much consensus gets distrusted. While we can discard those ideas as irrational conspiracy theories, I conclude that these findings have important implications for the way we deal with and communicate about complex societal problems. Next to keeping things simple and clear, as crisis/risk/science communication holds, we need to allow for uncertainty, critique and epistemic diversity as well.
Klasik Türk Şiirinde “İrem” Kelimesinin Kullanımı Üzerine
Authors: ÇOLAK Ahmet
Published: September 2023

Şair, malzemesi kelime, harcı hayal dünyası olan sanatkârdır. En önemli eseri ise şiirleridir. Şairler bu şiirleri kullandıkları kelime ve kavramlara geniş anlam katmanları katarak oluşturur. Çağrışımlarla kelimelere yeni anlamlar kazandırarak orijinal, başarılı ve üstün olma gibi gaye ve düşünceleri vardır. Bu kelimeler, kavramlar ve hayaller incelendiğinde şairin sahip olduğu düşünce dünyası keşfedilebilir. Ancak divan şairine yönelik bu keşfi yapabilmek için araştırmacıların bir bilgi birikimine sahip olmaları gerekir. Klasik Türk şiirinin kelime hazinesi, edebi sanatları, sembolleri, aruz gibi kuralları araştırmacının bilgi birikimi içerisinde olması gerekenlerden sadece bazılarıdır. Bu birikim sonrası derin, zengin ve farklı hayaller anlaşılabilir hâle gelir. İrem kelimesinin kullanımı üzerine hazırlanan bu çalışmada şairin hayal dünyası ile anlam zenginliği kazanmış olan “irem" kelimesinin katmanlı anlamları tespit edilmeye çalışılacaktır. İrem, "cennete özenilerek Şeddâd tarafından yaptırılmış yer" anlamındadır. Kur’ân-ı Kerim’de bahsolunan İrem oradaki şekli ile divan şiirinde sıklıkla anılır hâle gelmiştir. Dini ilimlerce olumsuz olarak tanımlanan İrem, edebi metinlerde istisnalar dışında pozitif bir anlama kavuşmuş ve mutluluk uyandıracak şekilde kullanılmıştır. Şiirlerde ise daha ziyade doğal güzellik, buluşma yeri, sevgilinin özelliği gibi manalara gelecek şekilde kullanılır. Çalışmada örnekler çerçevesinde bütün bu geniş anlam dairesi ortaya konulmaya çalışılmış ve irem kelimesinin kapsamı incelenmiştir. Bu inceleme, irem kelimesi ile doğrudan veya ikincil olarak etkileşim hâlindeki kelimeler arasındaki bağlama odaklanılarak yapılmıştır
Relationship between the level of education with the quality employee work in fish processing companies in PT. Triko Bina Nusantara in Dongkala Village, Pasarwajo District, Buton Regency
Authors: Lita Wa , Mansyur Akhmad , Budiyanto  , Patadjai Andi Besse , Patiung Seventry Meliana
Published: September 2023

This study aims to determine the relationship between the level of education with the quality of work of employees at PT. Triko Bina Nusantara. this research was conducted in Dongkala village, Pasarwajo district, Buton regency during September to October 2020. the method used in this study is the census method, with a total of 30 respondents. the analysis used is chi-square. the data consists of the level of education and quality of work of employees. the results showed that the relationship between the level of education and the quality of work has a significance value of 0,319>(0,05), which means that there is no relationship between the level of education with the quality of work of employees at PT. Triko Bina Nusanatara.
Fisherman productivity of bottom gill net and drift gill net during covid-19 in Napabalano Sub- District Napabalano District Muna Regency
Authors: Piliana Wa Ode , Gunawan Fandy Rahmad , Lawelle Sjamsu Alam , Yusuf Sarini , Annaastasia Nurhuda
Published: September 2023

This study aims to determine the productivity of fisherman who use bottom gill nets and drift gill nets during the Covid-19 period. This research was conducted in Napabalano Village, Napabalano District, Muna Regency from April to May 2022. The sampling method used was the census method with the number of respondents being 8 bottom gill net fisherman and 8 drift gill net fisherman. Data was collected trough interviews, observation and documentation. Analysis of the data used is the productivity Analysis. The results of the study showed that the productivity obtained by bottom gill net fisherman averaged 2.42, meanwhile, the productivity obtained by drift gill net fisherman is 3.01 on average. Thus, the two fishing gears are declared productive because the average productivity value is >1.
Analisis struktur pasar rumput laut di Kabupaten Buton Selatan
Authors: Kardianto La Ode , Sarinah  , Riani Irdam , Siang Roslindah Daeng , Sidiq Azwar
Published: September 2023

Penelitian ini bertujuan untuk mengetahui struktur pasar rumput laut. Teknik penarikan sampel dalam penelitian ini adalah menggunakan rumus Slovin dengan standar eror sebesar 10%. Metode analisis struktur pasar   rumput laut meliputi pangsa pasar,  hambatan keluar  masuk  pasar dan pengetahuan pasar rumput laut. Hasil penelitian menunjukkan bahwa struktur pasar rumput laut yang ada di Kabupaten Buton Selata adalah pangsa pasar 2 pedagang pengumpul memiliki selisih 44% dari total penjualan, hambatan untuk masuk kepemasaran rumput laut cukup besar karena nilai MES diatas 10%. Penentuan harga rumput laut ditentukan oleh pedagang pengumpul kota yang sudah disesuaikan dengan harga penjualan rumput laut di Makassar. Oleh karena itu, praktek penentuan harga yang terjadi dalam pemasaran rumput laut tersebut mengarah kepada pasar oligopsoni.
Analisis keuntungan pelaku usaha kerang kalandue (Polymesoda erosa) pada ekosistem mangrove di Kelurahan Korumba Kecamatan Mandonga Kota Kendari
Authors: Firdaus  , Nurdiana A  , Piliana Wa Ode , Rosmawati  , La Ola La Onu , Patiung Seventry Meliana
Published: September 2023

Penelitian ini bertujuan untuk mengetahui total biaya, penerimaan dan keuntungan pada penangkapan dan pemasaran kerang mangrove (Polymesoda erosa) di ekosistem mangrove. Penelitian ini dilaksanakan pada Bulan April 2022 di Kelurahan Korumba Kecamatan Mandonga Kota Kendari. Metode penarikan sampel yang digunakan yaitu metode sensus dengan jumlah responden 8 orang nelayan penangkap kerang mangrove. Pengumpulan data dilakukan melalui wawancara, observasi dan dokumentasi. Analisis data yang digunakan adalah analisis biaya, analisis penerimaan dan analisis keuntungan. Penangkapan kerang dilakukan oleh suami sedangkan istri nelayan bertanggung jawab untuk melakukan penjualan kerang.  Sebagian kecil hasil tangkapan diambil untuk konsumsi pribadi dan sebagian besar dijual. Jumlah tangkapan rata-rata perbulan adalah 276 kg.  Hasil analisis menunjukan bahwa rata-rata total biaya per bulan yang dikeluarkan sebesar Rp 488.150. Besaran rata-rata penerimaan per bulan adalah Rp1.381.875. Rata-rata keuntungan per bulan yang diperoleh sebesar Rp 876.100. Artinya penangkapan kerang mangrove pada ekosistem mangrove di Kelurahan Korumba Kecamatan Mandonga Kota Kendari menguntungkan.
TINJAUAN TEORITIS PENGARUH PERUBAHAN POLA CURAH HUJAN DALAM KAITANNYA DENGAN ASURANSI USAHA TANI BAWANG MERAH
Authors: Prabowo Agung , Sukono  , Mamat Mustafa
Published: September 2023

Usaha pada sektor pertanian seperti padi, jagung, bawang merah, tembakau, kakao dan lain-lain memiliki risiko kerugian berupa penurunan hasil produksi yang berakibat pada penurunan pendapatan petani. Risiko kerugian tersebut dapat disebabkan oleh berbagai faktor di antaranya curah hujan, suhu, kecepatan angin dan kelembaban udara yang semuanya merupakan unsur-unsur pembentuk iklim/cuaca. Penelitian ini bertujuan menyajikan alur riset pemodelan asuransi usaha tani khususnya bawang merah terkait dengan kondisi ekstrim basah dan kering akibat anomali cuaca. Penelitian yang dilakukan berkutat pada studi pustaka terkait dengan kondisi ekstrim basah dan kering tersebut. Sebagai kesimpulan, berdasarkan riset pustaka yang dilakukan, tanaman bawang merah tetap dapat tumbuh baik pada curah hujan normal, dan pada saat curah hujan tidak normal (kondisi kering atau basah), maka menanam bawang merah mempunyai peluang untuk gagal. Hal ini memberikan kesempatan pada perusahaan asuransi untuk mengembangkan Asuransi Usaha Tani Bawang Merah (AUTBM) pada kedua kondisi tersebut.
Analisis Iklim Mikro Kawasan Berdasarkan Kerapatan Bangunan dan Vegetasi di Kawasan Institut Teknologi Bandung (ITB) Kampus Ganesha Bagian Selatan
Authors: Annisa Binti Nolasari  , Wardatut Toyyibah  , Kurniawan Abhi , Dyah Rembulan W.S  , M. Fadhlul Wafi Chandra  , M. Donny Koerniawan
Published: September 2023

Penelitian ini dilakukan untuk mengetahui parameter dari lingkungan luar ruangan yang nyaman dan sejuk, terutama di negara tropis seperti Indonesia. Namun, belum ada penelitian sebelumnya yang menganalisis hubungan antara konfigurasi bangunan, kerapatan vegetasi, dan kondisi iklim di kawasan kampus, seperti di kawasan Institut Teknologi Bandung kampus Ganesha. Oleh karena itu, penelitian ini bertujuan untuk mengidentifikasi dan menganalisis iklim mikro di kampus ITB Ganesha. Faktor kenyamanan termal yang dipengaruhi oleh konfigurasi bangunan dan kerapatan vegetasi dianalisis untuk memahami hubungan antara faktor-faktor tersebut dengan kondisi iklim mikro di dalam kampus. Penelitian ini dilakukan menggunakan metode kuantitatif berupa simulasi menggunakan software ENVI-met yang kemudian dilakukan perbandingan antara tiap karakteristik kawasan dengan data hasil simulasi. Hasil penelitian menunjukkan bahwa area dengan kerapatan vegetasi yang lebih tinggi serta bangunan yang lebih rendah dan teratur memiliki kondisi iklim mikro yang lebih sejuk dan nyaman dibanding area dengan kerapatan vegetasi yang lebih rendah serta konfigurasi bangunan yang lebih tinggi dan padat. Penemuan ini dapat memberikan wawasan bagi perencanaan dan pengembangan kawasan kampus, serta memberikan kontribusi dalam menciptakan lingkungan yang nyaman dan sehat di kampus ITB Ganesha dalam menghadapi perubahan iklim global. Selain itu, penelitian ini juga dapat menjadi referensi bagi pengambil keputusan di bidang perencanaan kota dan lingkungan untuk meningkatkan kualitas kehidupan manusia melalui perbaikan kualitas lingkungan luar ruangan.
In vitro induction of neutrophil extracellular traps by SARS-CoV-2 is biased by extracellular mitochondria
Authors: Janko Jakub , Sláviková Monika , Klempa Boris , Celec Peter , Pastorek Michal
Published: September 2023

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has a highly variable course that is dependent on the host immune system reaction. Lung tissue damage, endothelial dysfunction, and microthrombosis in severe COVID-19 is linked to neutrophilia and the production of neutrophil extracellular traps (NETs). Previous studies have shown that NETs are involved in the pathology of COVID-19 and that the virus itself induces NET formation, although the underlying mechanisms are not clear. In this study, we aimed to investigate the induction of NETs by SARS-CoV-2 in vitro. We have found that both, infectious and heat-inactivated virus induce NETs formation. Surprisingly, cell culture media derived from uninfected Vero cells exhibit similar potency. This suggests that NET inducers other than the virus might be involved. Mitochondria released from dying cells during SARS-CoV-2 infection acting as damage-associated molecular patterns (DAMPs) were identified as potential contributors to neutrophil activation and NET formation. Our findings point to an important source of bias when analyzing NETs induction by SARS-CoV-2 in vitro, but also the immune reaction to viruses in general. Further implications for the understanding of COVID-19 pathogenesis remain to be elucidated.
Hybrid surgery management challenges of a Behcet's disease patient with recurrence of aortic aneurysms: a case report
Authors: Chen Guo Xin , Wan Jiang Hong , Chen Chu Wen , Huang Bin
Published: September 2023

Background

Behcet's disease is a vasculitis of unknown origin that can involve multiple organs or tissues. Aneurysm or pseudoaneurysm, also one of the complications of Behcet's disease, is usually accompanied by a poor prognosis. Surgery is usually accompanied by a high risk of complications, such as the recurrence of anastomotic pseudoaneurysms and blockage of the target vessel. Using hybrid surgery, we successfully treated a complex and recurrent abdominal aortic pseudoaneurysm in a patient with BD.

Methods

We report a 32-year-old female diagnosed with Behcet's disease with recurrent thoracoabdominal aortic aneurysm. Adequate immunotherapy was given during the perioperative period. The splanchnic artery branches were reconstructed, and the aneurysm was sequestered with endovascular repair. The patient recovered uneventfully and was discharged from the hospital 8 days after hybrid surgery. At the 60-month follow-up, no aneurysm was observed, the stent had no displacement or internal leakage, and the reconstructed blood vessels were unobstructed.

Conclusion

Hybrid surgery could be a feasible and effective strategy for BD aneurysms. Adequate preoperative and postoperative immunotherapy with arterial anastomosis away from the diseased artery may be the key to success.
Modelling the pathology and treatment of cardiac fibrosis in vascularised atrial and ventricular cardiac microtissues
Authors: Reyat Jasmeet S. , di Maio Alessandro , Grygielska Beata , Pike Jeremy , Kemble Samuel , Rodriguez-Romero Antonio , Simoglou Karali Christina , Croft Adam P. , Psaila Bethan , Simões Filipa , Rayes Julie , Khan Abdullah O.
Published: September 2023

Introduction

Recent advances in human cardiac 3D approaches have yielded progressively more complex and physiologically relevant culture systems. However, their application in the study of complex pathological processes, such as inflammation and fibrosis, and their utility as models for drug development have been thus far limited.

Methods

In this work, we report the development of chamber-specific, vascularised human induced pluripotent stem cell-derived cardiac microtissues, which allow for the multi-parametric assessment of cardiac fibrosis.

Results

We demonstrate the generation of a robust vascular system in the microtissues composed of endothelial cells, fibroblasts and atrial or ventricular cardiomyocytes that exhibit gene expression signatures, architectural, and electrophysiological resemblance to in vivo-derived anatomical cardiac tissues. Following pro-fibrotic stimulation using TGFβ, cardiac microtissues recapitulated hallmarks of cardiac fibrosis, including myofibroblast activation and collagen deposition. A study of Ca2+ dynamics in fibrotic microtissues using optical mapping revealed prolonged Ca2+ decay, reflecting cardiomyocyte dysfunction, which is linked to the severity of fibrosis. This phenotype could be reversed by TGFβ receptor inhibition or by using the BET bromodomain inhibitor, JQ1.

Discussion

In conclusion, we present a novel methodology for the generation of chamber-specific cardiac microtissues that is highly scalable and allows for the multi-parametric assessment of cardiac remodelling and pharmacological screening.
Sediment variability in intermittently extracted sandbanks in the Belgian part of the North Sea
Authors: Kint Lars , Barette Florian , Degrendele Koen , Roche Marc , Van Lancker Vera
Published: September 2023

Introduction: In the Belgian part of the North Sea, sand for beach and foreshore nourishments is extracted from the Hinder Banks, about 40 km offshore. The extraction is not screened for shells and other very coarse material, as is the case for sand extraction for industrial use. Intermittent but intensive sand extraction took place from 2012 to 2019 on the Oosthinder, and since 2019 on the Noordhinder.

Methods: To better understand sediment variability during human-induced disturbance of the seabed, both sandbanks have been monitored using multibeam bathymetry and backscatter, and Reineck box cores on board of the Research Vessel Belgica A962. Acoustic and sediment data were preferably jointly collected within a one-week period to substantiate the interrelationships.

Result: Very well-sorted medium sands with low backscatter values (−28 to −33 dB) are detected near the top of the sandbank, while (moderately) well-sorted coarser sands and shell fragments with high backscatter values (−20 to −24 dB) are detected in the deeper parts of the sandbank slope. Although natural conditions such as sandwave movement and storms may mask early sediment changes, 7 years of intermittent and intensive sand extraction on the upper parts of a gentle sandbank slope caused a seabed deepening of at least 2 m, a backscatter decrease between 5 and 8 dB, a reduction in carbonate content by 1%–5% and an increase in organic matter content by 1%–2%. Two years after the cessation of the operations, the carbonate content increased and organic matter content decreased by 1%–2%.

Discussion: Natural variability of sediments remains dependent on depth and geomorphology in both undisturbed, as well as recently and longer disturbed sandbank systems. However, a spreading of the more homogeneous, very well-sorted medium sands with limited carbonate content from the sandbank crest to the upper parts of the sandbank slope was observed, as sand extraction progressed. Subsequently, possible first signs of a slow return to the original sediment characteristics were detected.
Transient electromagnetic detection and numerical simulation analysis of the deformation characteristics of an old goaf in an alpine coal mine area
Authors: Wei Zhan-Xi , Dong Jian-Hui , Zhao Meng , Xie Fei-Hong , Li Ying-Jun , Feng Lin
Published: September 2023

In this paper, Qinghai alpine mining area is taken as the research object to explore the deformation characteristics of overlying strata in alpine mining area, so as to prevent geological disasters caused by over-exploitation in alpine mining area. The location of old goaf in coal mine is detected by transient electromagnetic method, and the results are used for numerical simulation. The numerical simulation results show that after coal seam mining, the stress gradually increases from the surface to the bottom, and the stress concentration occurs at both ends and the middle of the mining area. The displacement change area is highly coincident with the stress concentration area, and the displacement monitoring map is consistent with the horizontal displacement cloud map analysis, showing a “U” type distribution. The research results have certain reference value for the future study of surface mining subsidence in inclined coal seams.
Optimizing mass eruption rate estimates by combining simple plume models
Authors: Dürig Tobias , Schmidt Louise S. , Dioguardi Fabio
Published: September 2023

Tephra injected into the atmosphere by volcanic ash plumes poses one of the key hazards in explosive eruptions. Forecasting the atmospheric dispersal of volcanic ash requires good knowledge of the current eruption source parameters, in particular of the mass eruption rate (MER), which quantifies the mass flow rate of gas and tephra at the vent. Since this parameter cannot be directly measured in real-time, monitoring efforts aim to assess the MER indirectly, for example, by applying plume models that link the (relatively easily detectable) plume height with the mass flux at the vent. By comparing the model estimates with independently acquired fallout measurements from the 130 eruptions listed in the Independent Volcanic Eruption Source Parameter Archive (Aubry et al., J. Volcanol. Geotherm. Res., 2021, 417), we tested the success rates of six 0D plume models along with four different modelling approaches with the aim to optimize MER prediction. According to our findings, instead of simply relying on the application of one plume model for all situations, the accuracy of MER forecast can be increased by mixing the plume models via model weight factors when these factors are appropriately selected. The optimal choice of model weight factors depends on the availability and type of volcanological and meteorological information for the eruption monitored. A decision tree is presented that assists the reader in finding the optimal modelling strategy to ascertain highest MER forecast accuracy.
Effects of insulin therapy optimization with sensor augmented pumps on glycemic control and body composition in people with cystic fibrosis-related diabetes
Authors: Grancini V. , Alicandro G. , Porcaro L. L. , Zazzeron L. , Gramegna A. , Morlacchi L. C. , Rossetti V. , Gaglio A. , Resi V. , Daccò V. , Blasi F. , Orsi E.
Published: September 2023

Objective

Cystic fibrosis (CF)-related diabetes (CFRD) resulting from partial-to-complete insulin deficiency occurs in 40-50% of adults with CF. In people with CFRD, poor glycemic control leads to a catabolic state that may aggravate CF-induced nutritional impairment and loss of muscle mass. Sensor augmented pump (SAP) therapy may improve glycemic control as compared to multiple daily injection (MDI) therapy.

Research design and methods

This non-randomized clinical trial was aimed at evaluating the effects of insulin therapy optimization with SAP therapy, combined with a structured educational program, on glycemic control and body composition in individuals with insulin-requiring CFRD. Of 46 participants who were offered to switch from MDI to SAP therapy, 20 accepted and 26 continued the MDI therapy. Baseline demographic and clinical characteristics were balanced between groups using a propensity score-based overlap weighting procedure and weighted mixed-effects regression models were used to estimate changes in study outcomes.

Results

After 24 months changes in HbA1c were: -1.1% (-12.1 mmol/mol) (95% CI: -1.5; -0.8) and -0.1% (-1 mmol/mol) (95% CI: -0.5; 0.3) in the SAP and MDI therapy group, respectively, with a between-group difference of -1.0 (-10 mmol/mol) (-1.5; -0.5). SAP therapy was also associated with a decrease in mean glucose (between group difference: -32 mg/dL; 95% CI: -44; -20) and an increase in TIR (between group difference: 19.3%; 95% CI 13.9; 24.7) and in fat-free mass (between group difference: +5.5 Kg, 95% CI: 3.2; 7.8).

Conclusion

Therapy optimization with SAP led to a significant improvement in glycemic control, which was associated with an increase in fat-free mass.
Simultaneous series and shunt earth fault detection and classification using the Clarke transform for power transmission systems under different fault scenarios
Authors: Esmail Ehab M. , Alsaif Faisal , Abdel Aleem Shady H. E. , Abdelaziz Almoataz Y. , Yadav Anamika , El-Shahat Adel
Published: September 2023

For high-voltage (symmetric and non-symmetric) transmission networks, detecting simultaneous faults utilizing a single-end-based scheme is complex. In this regard, this paper suggests novel schemes for detecting simultaneous faults. The proposed schemes comprise two different stages: fault detection and identification and fault classification. The first proposed scheme needs communication links among both ends (sending and receiving) to detect and identify the fault. This communication link between both ends is used to send and receive three-phase current magnitudes for sending and receiving ends in the proposed fault detection (PFD) unit at both ends. The second proposed scheme starts with proposed fault classification (PFC) units at both ends. The proposed classification technique applies the Clarke transform on local current signals to classify the open conductor and simultaneous faults. The sign of all current Clarke components is the primary key for distinguishing between all types of simultaneous low-impedance and high-impedance faults. The fault detection time of the proposed schemes reaches 20 ms. The alternative transient program (ATP) package simulates a 500 kV–150-mile transmission line. The simulation studies are carried out to assess the suggested fault detection and identification and fault classification scheme performance under various OCFs and simultaneous earth faults in un-transposed and transposed TLs. The behavior of the proposed schemes is tested and validated by considering different fault scenarios with varying locations of fault, inception angles, fault resistance, and noise. A comparative study of the proposed schemes and other techniques is presented. Furthermore, the proposed schemes are extended to another transmission line, such as the 400 kV–144 km line. The obtained results demonstrated the effectiveness and reliability of the proposed scheme in correctly detecting simultaneous faults, low-impedance faults, and high-impedance faults.
Multi-stage low-carbon planning of an integrated energy system considering demand response
Authors: Huan Jiajia , Ding Qiaoyi , Yu Tao , Cheng Yusi
Published: September 2023

In the context of energy crisis, the development of low-carbon integrated energy systems has become a prominent research area. This article addresses the challenges posed by high energy consumption and emissions in integrated energy systems by proposing a multi-stage planning method for low-carbon integrated energy that considers load time transfer characteristics. The first step involves examining the time transfer characteristics of demand response and analyzing the economic benefits of integrated energy systems participating in the electricity–carbon market. Subsequently, a multi-stage green low-carbon planning model for the integrated energy system is constructed. To validate the effectiveness of the proposed model, actual calculation results are obtained. These results demonstrate that the demand response, specifically in data centers, can significantly reduce the operational costs of integrated energy systems. Furthermore, the multi-stage low-carbon planning approach is shown to be more reasonable and economically beneficial compared to single-stage planning. Overall, this research article provides insights into the development of low-carbon integrated energy systems within the context of energy crisis. By considering load time transfer characteristics and employing a multi-stage planning method, this article highlights the potential for reducing costs and improving the overall efficiency of integrated energy systems.
Interleukin-36γ is causative for liver damage upon infection with Rift Valley fever virus in type I interferon receptor-deficient mice
Authors: Anzaghe Martina , Niles Marc A. , Korotkova Eugenia , Dominguez Monica , Kronhart Stefanie , Ortega Iannazzo Samira , Bechmann Ingo , Bachmann Malte , Mühl Heiko , Kochs Georg , Waibler Zoe
Published: September 2023

Type I interferons (IFN) are pro-inflammatory cytokines which can also exert anti-inflammatory effects via the regulation of interleukin (IL)-1 family members. Several studies showed that interferon receptor (IFNAR)-deficient mice develop severe liver damage upon treatment with artificial agonists such as acetaminophen or polyinosinic:polycytidylic acid. In order to investigate if these mechanisms also play a role in an acute viral infection, experiments with the Bunyaviridae family member Rift Valley fever virus (RVFV) were performed. Upon RVFV clone (cl)13 infection, IFNAR-deficient mice develop a severe liver injury as indicated by high activity of serum alanine aminotransferase (ALT) and histological analyses. Infected IFNAR-/- mice expressed high amounts of IL-36γ within the liver, which was not observed in infected wildtype (WT) animals. In line with this, treatment of WT mice with recombinant IL-36γ induced ALT activity. Furthermore, administration of an IL-36 receptor antagonist prior to infection prevented the formation of liver injury in IFNAR-/- mice, indicating that IL-36γ is causative for the observed liver damage. Mice deficient for adaptor molecules of certain pattern recognition receptors indicated that IL-36γ induction was dependent on mitochondrial antiviral-signaling protein and the retinoic acid-inducible gene-I-like receptor. Consequently, cell type-specific IFNAR knockouts revealed that type I IFN signaling in myeloid cells is critical in order to prevent IL-36γ expression and liver injury upon viral infection. Our data demonstrate an anti-inflammatory role of type I IFN in a model for virus-induced hepatitis by preventing the expression of the novel IL-1 family member IL-36γ.
Persistence of immunological memory as a potential correlate of long-term, vaccine-induced protection against Ebola virus disease in humans
Authors: McLean Chelsea , Dijkman Karin , Gaddah Auguste , Keshinro Babajide , Katwere Michael , Douoguih Macaya , Robinson Cynthia , Solforosi Laura , Czapska-Casey Dominika , Dekking Liesbeth , Wollmann Yvonne , Volkmann Ariane , Pau Maria Grazia , Callendret Benoit , Sadoff Jerry , Schuitemaker Hanneke , Zahn Roland , Luhn Kerstin , Hendriks Jenny , Roozendaal Ramon
Published: September 2023

Introduction

In the absence of clinical efficacy data, vaccine protective effect can be extrapolated from animals to humans, using an immunological biomarker in humans that correlates with protection in animals, in a statistical approach called immunobridging. Such an immunobridging approach was previously used to infer the likely protective effect of the heterologous two-dose Ad26.ZEBOV, MVA-BN-Filo Ebola vaccine regimen. However, this immunobridging model does not provide information on how the persistence of the vaccine-induced immune response relates to durability of protection in humans.

Methods and results

In both humans and non-human primates, vaccine-induced circulating antibody levels appear to be very stable after an initial phase of contraction and are maintained for at least 3.8 years in humans (and at least 1.3 years in non-human primates). Immunological memory was also maintained over this period, as shown by the kinetics and magnitude of the anamnestic response following re-exposure to the Ebola virus glycoprotein antigen via booster vaccination with Ad26.ZEBOV in humans. In non-human primates, immunological memory was also formed as shown by an anamnestic response after high-dose, intramuscular injection with Ebola virus, but was not sufficient for protection against Ebola virus disease at later timepoints due to a decline in circulating antibodies and the fast kinetics of disease in the non-human primates model. Booster vaccination within three days of subsequent Ebola virus challenge in non-human primates resulted in protection from Ebola virus disease, i.e. before the anamnestic response was fully developed.

Discussion

Humans infected with Ebola virus may benefit from the anamnestic response to prevent disease progression, as the incubation time is longer and progression of Ebola virus disease is slower as compared to non-human primates. Therefore, the persistence of vaccine-induced immune memory could be considered as a potential correlate of long-term protection against Ebola virus disease in humans, without the need for a booster.
Comparison of crystalloid and colloid co-load combined with norepinephrine prophylaxis on post-spinal anesthesia hypotension during cesarean delivery: a randomized sequential allocation dose-finding study
Authors: Chen Yi , Xu Xiangzhao , Qin Rui , Guo Lei , Ni Xinli
Published: September 2023

Background

Fluid loading is an essential component of treatment for reducing the incidence of post-spinal anesthesia hypotension and is necessary to maintain intravascular volume, perfuse tissues, and control spinal anesthesia hypotension after sympathetic blockade. We performed a randomized sequential allocation dose-finding study to compare the effects of 10 mL/kg crystalloid and 6% hydroxyethyl starch (130/0.4) co-load on the ED90 of prophylactic norepinephrine infusion for preventing post-spinal anesthesia hypotension during cesarean delivery.

Methods

Eighty patients were randomly allocated to receive either a 10 mL/kg crystalloid (Crystalloid Group, n = 40) or 6% hydroxyethyl starch (130/0.4) (Colloid Group, n = 40) co-load combined with prophylactic norepinephrine infusion during spinal anesthesia for cesarean delivery. The first patient received an initial prophylactic norepinephrine infusion rate of 0.025 μg/kg/min. Subsequent patients received a 0.005 μg/kg/min gradient dose of prophylactic norepinephrine. This dose was administered as a gradient based on its effectiveness for preventing post-spinal anesthesia hypotension (defined as SBP < 80% of baseline value) and determined by the up-and-down sequential allocation methodology. The primary study outcome was the ED90 of prophylactic norepinephrine infusion. Secondary outcomes included the incidence of post-spinal anesthesia hypotension, bradycardia, hypertension, Apgar scores, and umbilical artery blood gas values were also measured.

Results

The ED90 values of prophylactic norepinephrine infusion for preventing post-spinal anesthesia hypotension during cesarean delivery were 0.063 μg (95% CI: 0.050 to 0.064) and 0.062 μg (95% CI: 0.045 to 0.064) using isotonic regression analysis, and 0.068 μg (95% CI: 0.056 to 0.353) and 0.060 μg (95% CI: 0.050 to 3.590) using probit regression analysis in the Crystalloid Group and Colloid Group, respectively. The secondary outcomes were comparable between the two groups.

Conclusion

The administration of a 10 mL/kg 6% hydroxyethyl starch (130/0.4) does not provide additional benefits compared to crystalloid co-load in reducing the ED90 of prophylactic norepinephrine infusion for preventing post-spinal anesthesia hypotension during cesarean delivery.
Sporulation conditions influence the surface and adhesion properties of Bacillus subtilis spores
Authors: Hamiot Audrey , Lemy Christelle , Krzewinski Frederic , Faille Christine , Dubois Thomas
Published: September 2023

Spore-forming bacteria of the Bacillus subtilis group are responsible for recurrent contamination of processing lines in the food industry which can lead to food spoilage. The persistence of B. subtilis would be due to the high resistance of spores to extreme environmental condition and their propensity to contaminate surfaces. While it is well known that sporulation conditions modulate spore resistance properties, little is known about their effect on surface and adhesion properties. Here, we studied the impact of 13 sporulation conditions on the surface and adhesion properties of B. subtilis 168 spores. We showed that Ca2+ or Mg2+ depletion, lower oxygen availability, acidic pH as well as oxidative stresses during sporulation lead to the release of more hydrophobic and adherent spores. The consequences of these sporulation conditions on crust composition in carbohydrates and proteins were also evaluated. The crust glycans of spores produced in a sporulation medium depleted in Ca2+ or Mg2+ or oxygen-limited conditions were impaired and contained lower amounts of rhamnose and legionaminic acid. In addition, we showed that lower oxygen availability or addition of hydrogen peroxide during sporulation decreases the relative amount of two crust proteins (CgeA and CotY) and the changes observed in these conditions could be due to transcriptional repression of genes involved in crust synthesis in late stationary phase. The fact that sporulation conditions affect the ease with which spores can contaminate surfaces could explain the frequent and recurrent presence of B. subtilis spores in food processing lines.