bluuebunny/crossref_metadata_2025 huggingface dataset:
Multilayer coatings containing red ginseng dietary fibre improve the survivability and stability of probiotic bacteria
Authors: Jeon Hyeon Ji , You Seung‐hwan , Kwon Mijin , Shin Minhye , Kim Sang‐Kyu , Jung Young Hoon
Published: January 2023

Maintaining the viability of probiotics is critical for maximising their functionality. In this study, tara gum, basic amino acids, rice protein and red ginseng dietary fibre were sequentially applied to encapsulate various probiotic strains to improve their viability and enhance their adhesion to intestinal cells. Compared with uncoated control strains, multilayer‐coated probiotic strains exhibited higher survival rates under low pH conditions (maximum survival: 81.8% for Lactobacillus casei) and bile acid conditions (>50% survival for Lactobacillus rhamnosus). Multicoating also increased the ability of probiotic strains to adhere to intestinal epithelial cells. For example, encapsulated L. reuteri exhibited 56.9% adhesion, which was approximately four‐fold higher than that of the uncoated strain. Moreover, compared with the uncoated strains, multicoated strains exhibited significantly improved storage stability under various temperatures after freeze‐drying. Therefore, the quadruple‐coating technology used in this study enhances the viability and stability of probiotic strains, which is a key advantage from the industrial perspective.
Potential cost‐effectiveness of e‐health interventions for treating overweight and obesity in Australian adolescents
Authors: Carrello Joseph , Hayes Alison , Baur Louise A. , Lung Thomas
Published: January 2023

Background

E‐health, defined as the use of information and communication technologies to improve healthcare delivery and health outcomes, has been promoted as a cost‐effective strategy to treat adolescent overweight and obesity. However, evidence supporting this claim is lacking.

Objectives

Assess the potential cost‐effectiveness of a hypothetical e‐health intervention for adolescents with overweight and obesity.

Methods

The costs and effect size (BMI reduction) of the hypothetical intervention were sourced from recent systematic reviews. Using a micro‐simulation model with a lifetime time horizon, we conducted a modelled cost‐utility analysis of the intervention compared to a ‘do‐nothing’ approach. To explore uncertainty, we conducted bootstrapping on individual‐level costs and quality‐adjusted life years (QALYs) and performed multiple one‐way sensitivity analyses.

Results

The incremental cost‐effectiveness ratio (ICER) for the e‐health intervention was dominant (cheaper and more effective), with a 96% probability of being cost‐effective at a willingness‐to‐pay (WTP) of $50 000/QALY. The ICER remained dominant in all sensitivity analyses except when using the lower bounds of the hypothetical intervention effect size, which reduced the probability of cost‐effectiveness at a WTP of $50 000/QALY to 51%.

Conclusion

E‐health interventions for treatment of adolescent overweight and obesity demonstrate very good cost‐effectiveness potential and should be considered by healthcare decision makers. However, further research on the efficacy of such interventions is warranted to strengthen the case for investment.
Long‐term clinical and cost‐effectiveness of a therapist‐supported online remote behavioural intervention for tics in children and adolescents: extended 12‐ and 18‐month follow‐up of a single‐blind randomised controlled trial
Authors: Hollis Chris , Hall Charlotte L. , Khan Kareem , Jones Rebecca , Marston Louise , Le Novere Marie , Hunter Rachael , Andrén Per , Bennett Sophie D. , Brown Beverley J. , Chamberlain Liam R. , Davies E. Bethan , Evans Amber , Kouzoupi Natalia , McKenzie Caitlin , Sanderson Charlotte , Heyman Isobel , Kilgariff Joseph , Glazebrook Cristine , Mataix‐Cols David , Serlachius Eva , Murray Elizabeth , Murphy Tara
Published: January 2023

Background

Little is known about the long‐term effectiveness of behavioural therapy for tics. We aimed to assess the long‐term clinical and cost‐effectiveness of online therapist‐supported exposure and response prevention (ERP) therapy for tics 12 and 18 months after treatment initiation.

Methods

ORBIT (online remote behavioural intervention for tics) was a two‐arm (1:1 ratio), superiority, single‐blind, multicentre randomised controlled trial comparing online ERP for tics with online psychoeducation. The trial was conducted across two Child and Adolescent Mental Health Services in England. Participants were recruited from these two sites, across other clinics in England, or by self‐referral. This study was a naturalistic follow‐up of participants at 12‐ and 18‐month postrandomisation. Participants were permitted to use alternative treatments recommended by their clinician. The key outcome was the Yale Global Tic Severity Scale Total Tic Severity Score (YGTSS‐TTSS). A full economic evaluation was conducted. Registrations are ISRCTN (ISRCTN70758207); ClinicalTrials.gov (NCT03483493).

Results

Two hundred and twenty‐four participants were enrolled: 112 to ERP and 112 to psychoeducation. The sample was predominately male (177; 79%) and of white ethnicity (195; 87%). The ERP intervention reduced baseline YGTSS‐TTSS by 2.64 points (95% CI: −4.48 to −0.79) with an effect size of −0.36 (95% CI: −0.61 to −0.11) after 12 months and by 2.01 points (95% CI: −3.86 to −0.15) with an effect size of −0.27 (95% CI ‐0.52 to −0.02) after 18 months, compared with psychoeducation. Very few participants (<10%) started new tic treatment during follow‐up. The cost difference in ERP compared with psychoeducation was £304.94 (−139.41 to 749.29). At 18 months, the cost per QALY gained was £16,708 for ERP compared with psychoeducation.

Conclusions

Remotely delivered online ERP is a clinical and cost‐effective intervention with durable benefits extending for up to 18 months. This represents an efficient public mental health approach to increase access to behavioural therapy and improve outcomes for tics.
Feeding behaviour, locomotion rhythms and blood biochemistry of the neotropical red‐tail catfish (Phractocephalus hemioliopterus)
Authors: de Oliveira Guilherme Helder , Perez Ribeiro Paula Adriane , Prado Verônica Guimarães Landa , Bahiense Raphael Nogueira , Gamarano Pedro Gomes , de Oliveira Camila Gomes , de Almeida Freitas Débora , Costa Leandro Santos
Published: January 2023

The study evaluated the feeding behaviour ofPhractocephalus hemioliopterusthrough the animals' ability to adapt to the self‐feeding system, their preferred feeding times and locomotor activity, as well as the blood biochemistry of juveniles fed in a light/dark cycle. The study was carried out through two experiments, the first of which contained two phases. In experiment 1 – phase I, 24 juveniles (35.28 ± 0.62 g) were distributed in eight 48 l tanks. The tanks were equipped with a self‐feeding system and the experiment consisted of evaluating whether the animals were able to adapt to the self‐feeding system, as well as evaluating the preferred feeding times and locomotor activity of these animals. A feeding challenge to the animals was introduced in phase II, based on the results of phase I. The results of the first phase evidenced a nocturnal feeding preference. Thus, the feeding challenge consisted of measuring whether the animal would feed during the day and how long it would take to adapt. When the animals consumed 100% of the amount of feed provided daily, phase II was ended. In experiment 2, 24 juveniles ofP.hemioliopterus(182.00 ± 14.03 g) were distributed in eight 96 l tanks. This experiment consisted of two treatments with four repetitions, one with exclusive feeding during the middle of the light cycle and another with exclusive feeding in the middle of the dark cycle. At the end, blood samples were collected from the animals for blood biochemistry evaluations. In experiment 1 – phase I, the results showed that the fish adapted very well to the self‐feeding system and had a strictly nocturnal feeding behaviour and locomotor rhythm. When they were submitted to the feeding challenge in phase II, the feed intake was stabilized from the 17th day onwards, proportionally to the nocturnal consumption observed in the first phase, thus demonstrating feeding plasticity. In experiment 2, the feeding times influenced the animals' biochemical parameters. Animals fed during the night had higher values of cholesterol and triglycerides than animals fed during the day. It is concluded thatP.hemioliopterushas fast adaptability to a self‐feeding system, with strictly nocturnal feeding and locomotor behaviours. However, it has feeding plasticity, adapting its behaviour according to food availability. Blood biochemical parameters are influenced by the light/dark feeding cycle.
DiceCT for fishes: recommendations for pairing iodine contrast agents with μCT to visualize soft tissues in fishes
Authors: Kolmann Matthew A. , Nagesan Ramon S. , Andrews James V. , Borstein Samuel R. , Figueroa Rodrigo Tinoco , Singer Randal A. , Friedman Matt , López‐Fernández Hernán
Published: January 2023

Computed tomography (CT) scanning and other high‐throughput three‐dimensional (3D) visualization tools are transforming the ways we study morphology, ecology and evolutionary biology research beyond generating vast digital repositories of anatomical data. Contrast‐enhanced chemical staining methods, which render soft tissues radio‐opaque when coupled with CT scanning, encompass several approaches that are growing in popularity and versatility. Of these, the various diceCT techniques that use an iodine‐based solution like Lugol's have provided access to an array of morphological data sets spanning extant vertebrate lineages. This contribution outlines straightforward means for applying diceCT techniques to preserved museum specimens of cartilaginous and bony fishes, collectively representing half of vertebrate species diversity. This study contrasts the benefits of using either aqueous or ethylic Lugol's solutions and reports few differences between these methods with respect to the time required to achieve optimal tissue contrast. It also explores differences in minimum stain duration required for different body sizes and shapes and provides recommendations for staining specimens individually or in small batches. As reported by earlier studies, the authors note a decrease in pH during staining with either aqueous or ethylic Lugol's. Nonetheless, they could not replicate the drastic declines in pH reported elsewhere. They provide recommendations for researchers and collections staff on how to incorporate diceCT into existing curatorial practices, while offsetting risk to specimens. Finally, they outline how diceCT with Lugol's can aid ichthyologists of all kinds in visualizing anatomical structures of interest: from brains and gizzards to gas bladders and pharyngeal jaw muscles.
Behavioural responses of fishes to anthropogenic disturbances: Adaptive value and ecological consequences
Authors: Candolin Ulrika , Rahman Tawfiqur
Published: January 2023

Aquatic ecosystems are changing at an accelerating rate because of human activities. The changes alter the abundance and distribution of fishes, with potential consequences for ecosystem structure and function. Behavioural responses often underlie these changes in population dynamics, such as altered habitat choice or foraging activity. Here, we present a framework for understanding how and why behaviour is affected by human activities and how the behavioural responses in turn influence higher ecological levels. We further review the literature to assess the present state of the field and identify gaps in our knowledge. We begin with discussing the factors that determine how an individual responds to a change in the environment and whether the response is adaptive or not. In particular, we explain the importance of the evolutionary history of the species. We then search the literature to assess our current knowledge of the impact of human disturbances on the behaviour of fishes and the consequences for ecosystems. The search reveals that much attention has been directed to the impact of human activities on the behaviour of fishes, but that worryingly little is known about the consequences of these responses for populations, communities and ecosystems. Yet, behavioural responses can have profound ecological consequences given that behaviour underly many, if not most, species interactions. Thus, more attention should be paid to the mechanisms and pathways through which behavioural responses influence higher ecological levels. Such information is needed if we are to determine the ultimate effects of human activities on biodiversity and the function and stability of aquatic ecosystems.
The effects of ocean acidification on fishes – history and future outlook
Authors: Sundin Josefin
Published: January 2023

The effects of increased levels of carbon dioxide (CO2) on the Earth's temperature have been known since the end of the 19th century. It was long believed that the oceans' buffering capacity would counteract any effects of dissolved CO2 in marine environments, but during recent decades, many studies have reported detrimental effects of ocean acidification on aquatic organisms. The most prominent effects can be found within the field of behavioural ecology, e.g., complete reversal of predator avoidance behaviour in CO2‐exposed coral reef fish. Some of the studies have been very influential, receiving hundreds of citations over recent years. The results have also been conveyed to policymakers and publicized in prominent media outlets for the general public. Those extreme effects of ocean acidification on fish behaviour have, however, spurred controversy, given that more than a century of research suggests that there are few or no negative effects of elevated CO2 on fish physiology. This is due to sophisticated acid–base regulatory mechanisms that should enable their resilience to near‐future increases in CO2. In addition, an extreme “decline effect” has recently been shown in the literature regarding ocean acidification and fish behaviour, and independent research groups have been unable to replicate some of the most profound effects. Here, the author presents a brief historical overview on the effects of elevated CO2 and ocean acidification on fishes. This historical recap is warranted because earlier work, prior to a recent (c. 10 year) explosion in interest, is often overlooked in today's ocean acidification studies, despite its value to the field. Based on the historical data and the current knowledge status, the author suggests future strategies with the aim to improve research rigour and clarify the understanding of the effects of ocean acidification on fishes.
Genome‐scale angiosperm phylogenies based on nuclear, plastome, and mitochondrial datasets
Authors: Hu Hongyin , Sun Pengchuan , Yang Yongzhi , Ma Jianxiang , Liu Jianquan
Published: January 2023

Angiosperms dominate the Earth's ecosystems and provide most of the basic necessities for human life. The major angiosperm clades comprise 64 orders, as recognized by the APG IV classification. However, the phylogenetic relationships of angiosperms remain unclear, as phylogenetic trees with different topologies have been reconstructed depending on the sequence datasets utilized, from targeted genes to transcriptomes. Here, we used currently available de novo genome data to reconstruct the phylogenies of 366 angiosperm species from 241 genera belonging to 97 families across 43 of the 64 orders based on orthologous genes from the nuclear, plastid, and mitochondrial genomes of the same species with compatible datasets. The phylogenetic relationships were largely consistent with previously constructed phylogenies based on sequence variations in each genome type. However, there were major inconsistencies in the phylogenetic relationships of the five Mesangiospermae lineages when different genomes were examined. We discuss ways to address these inconsistencies, which could ultimately lead to the reconstruction of a comprehensive angiosperm tree of life. The angiosperm phylogenies presented here provide a basic framework for further updates and comparisons. These phylogenies can also be used as guides to examine the evolutionary trajectories among the three genome types during lineage radiation.
A critical update on the leptin‐melanocortin system
Authors: Lavoie Olivier , Michael Natalie Jane , Caron Alexandre
Published: January 2023

The discovery of leptin in 1994 was an “eureka moment” in the field of neurometabolism that provided new opportunities to better understand the central control of energy balance and glucose metabolism. Rapidly, a prevalent model in the field emerged that pro‐opiomelanocortin (POMC) neurons were key in promoting leptin's anorexigenic effects and that the arcuate nucleus of the hypothalamus (ARC) was a key region for the regulation of energy homeostasis. While this model inspired many important discoveries, a growing body of literature indicates that this model is now outdated. In this review, we re‐evaluate the hypothalamic leptin–melanocortin model in light of recent advances that directly tackle previous assumptions, with a particular focus on the ARC. We discuss how segregated and heterogeneous these neurons are, and examine how the development of modern approaches allowing spatiotemporal, intersectional, and chemogenetic manipulations of melanocortin neurons has allowed a better definition of the complexity of the leptin–melanocortin system. We review the importance of leptin in regulating glucose homeostasis, but not food intake, through direct actions on ARC POMC neurons. We further highlight how non‐POMC, GABAergic neurons mediate leptin's direct effects on energy balance and influence POMC neurons.

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Transactional sex among rural, justice‐involved Appalachian women who use drugs
Authors: Dickson Megan F. , Tillson Martha , Calvert Joseph M. , Staton Michele
Published: January 2023

Purpose

Transactional sex is associated with an array of other health risk behaviors and adverse health outcomes, including HIV. However, despite concerns regarding a potential HIV outbreak, there is limited understanding of transactional sex among rural Appalachians who engage in high‐risk behaviors. Thus, the current study describes the prevalence and correlates of transactional sex among a sample of rural, justice‐involved Appalachian women who use drugs.

Methods

Participants (N = 400) were randomly selected, screened, and interviewed face‐to‐face at 3 rural Appalachian jails in Kentucky. Bivariate analyses were used to examine differences between those who reported trading sex for drugs, money, goods, or services in the year prior to incarceration and those who had not, and multivariable logistic regression was used to examine independent correlates of transactional sex.

Findings

On average, participants were 33 years old with 11 years of education. They were predominantly White (99.0%), about half (43.7%) reported lifetime transactional sex, and 25.9% reported past year transactional sex. Past year transactional sex was positively associated with experiencing money problems, substance use problem severity, injection drug use, unprotected sex with a casual partner, and number of sexual partners in the year prior to incarceration.

Conclusions

Results suggest that transactional sex is fairly common among rural Appalachian women who use drugs and are justice‐involved and may signal other HIV‐related risk behaviors. Given limited service availability throughout rural Appalachia, findings emphasize a need for increased access to risk‐reduction interventions, including jail‐based interventions, to educate vulnerable, hard‐to‐reach populations on the risks associated with HIV.
Complementary belowground strategies underlie species coexistence in an early successional forest
Authors: Yi Ruojun , Liu Qianyuan , Yang Fengting , Dai Xiaoqin , Meng Shengwang , Fu Xiaoli , Li Shenggong , Kou Liang , Wang Huimin
Published: January 2023

Unravelling belowground strategies is critical for understanding species coexistence and successional dynamics; yet, our knowledge of nutrient acquisition strategies of forest species at different successional stages remains limited.

We measured morphological (diameter, specific root length, and root tissue density), architectural (branching ratio), physiological (ammonium, nitrate, and glycine uptake rates) root traits, and mycorrhizal colonisation rates of eight coexisting woody species in an early successional plantation forest in subtropical China.

By incorporating physiological uptake efficiency, we revealed a bi‐dimensional root economics space comprising of an ‘amount‐efficiency’ dimension represented by morphological and physiological traits, and a ‘self‐symbiosis’ dimension dominated by architectural and mycorrhizal traits. The early pioneer species relied on root–fungal symbiosis, developing densely branched roots with high mycorrhizal colonisation rates for foraging mobile soil nitrate. The late pioneer species invested in roots themselves and allocated effort towards improving uptake efficiency of less‐mobile ammonium. Within the root economics space, the covariation of axes with soil phosphorus availability also distinguished the strategy preference of the two successional groups.

These results demonstrate the importance of incorporating physiological uptake efficiency into root economics space, and reveal a trade‐off between expanding soil physical space exploration and improving physiological uptake efficiency for successional species coexistence in forests.
Coordinated histone variant H2A.Z eviction and H3.3 deposition control plant thermomorphogenesis
Authors: Zhao Fengyue , Xue Mande , Zhang Huairen , Li Hui , Zhao Ting , Jiang Danhua
Published: January 2023

Plants can sense temperature changes and adjust their development and morphology accordingly in a process called thermomorphogenesis. This phenotypic plasticity implies complex mechanisms regulating gene expression reprogramming in response to environmental alteration. Histone variants often associate with specific chromatin states; yet, how their deposition/eviction modulates transcriptional changes induced by environmental cues remains elusive.

In Arabidopsis thaliana, temperature elevation‐induced transcriptional activation at thermo‐responsive genes entails the chromatin eviction of a histone variant H2A.Z by INO80, which is recruited to these loci via interacting with a key thermomorphogenesis regulator PIF4.

Here, we show that both INO80 and the deposition chaperones of another histone variant H3.3 associate with ELF7, a critical component of the transcription elongator PAF1 complex. H3.3 promotes thermomorphogenesis and the high temperature‐enhanced RNA Pol II transcription at PIF4 targets, and it is broadly required for the H2A.Z removal‐induced gene activation. Reciprocally, INO80 and ELF7 regulate H3.3 deposition, and are necessary for the high temperature‐induced H3.3 enrichment at PIF4 targets.

Our findings demonstrate close coordination between H2A.Z eviction and H3.3 deposition in gene activation induced by high temperature, and pinpoint the importance of histone variants dynamics in transcriptional regulation.
Osteogenic mesenchymal stem cells/progenitors in the periodontium
Authors: Wen Wen , Pang Yu , Tian Yuyang , Xu Chunmei , Wang Jun , Wu Yafei , Xie Xudong
Published: January 2023

Periodontitis is the major cause of tooth loss in adults and is mainly characterized by alveolar bone destruction. Elucidating the mesenchymal stem cell (MSC)/progenitor populations of alveolar bone formation will provide valuable insights into regenerative approaches to clinical practice, such as endogenous regeneration and stem‐cell‐based tissue engineering therapies. Classically, MSCs residing in the bone marrow, periosteum, periodontal ligament (PDL), and even the gingiva are considered to be osteogenic progenitors. Furthermore, the contributions of MSCs expressing specific markers, including Gli1, Axin2, PTHrP, LepR, and α‐SMA, to alveolar bone formation have been studied using cell lineage tracing and gene knockout models. In this review, we describe the MSCs/progenitors of alveolar bone and the biological properties of different subpopulations of MSCs involved in alveolar bone development, remodeling, injury repair, and regeneration.
Ptip is essential for tooth development via regulating Wnt pathway
Authors: Liang Jianfei , Wang Jing , Ye Chen , Bai Yi , Tong Yibo , Li Yashu , Ji Yaoting , Zhang Yufeng
Published: January 2023

Objective

Epigenetic regulation plays important role in stem cell maintenance. Ptip was identified as epigenetic regulator, but the role in dental progenitor cells remains unclear.

Subjects and Methods

Dental mesenchymal progenitor cells were targeted by Sp7‐icre and visualized in mTmG; Sp7‐icre mice. The Ptipf/f; Sp7‐icre mice were generated and the phenotype of incisors and molars were shown by micro‐computerized tomography, scanning electron microscope, hematoxylin & eosin staining, and immunofluorescence. Dental mesenchymal progenitor cells were sorted by fluorescence‐activated cell sorting from lower incisors and RNA sequencing was performed.

Results

The Sp7‐icre targets dental mesenchymal progenitor cells in incisors and molars. The Ptipf/f; Sp7‐icre mice showed spontaneous fractures in the cusp of upper incisors and lower incisors at 3 weeks (w), compensative overgrowth of lower incisors at 1 month (M), and overgrowth extended to the outside at 2 M. The molars showed shortened roots. The functions of odontoblasts and dental mesenchymal progenitor cells were impaired. Mechanically, loss of Ptip activates the Wnt pathway and upregulates the expression of Wls in dental mesenchymal progenitor cells. Also, the regenerative ability of lower incisors was significantly impaired.

Conclusion

We first demonstrated that Ptip was crucial for tooth development via regulating Wnt signaling.
Athletes are not at greater risk for death by suicide: A review
Authors: Pichler Eva‐Maria , Ewers Simon , Ajdacic‐Gross Vladeta , Deutschmann Markus , Exner Jan , Kawohl Wolfram , Seifritz Erich , Claussen Malte Christian
Published: January 2023

Introduction

Suicide represents a major mental and public health issue. Elite athletes share certain individual and environmental characteristics that may increase their risk for mental illnesses, ultimately leading to suicide. This notion conflicts with the general perception of athletes, being the healthiest representatives of society.

Methods

A comprehensive literature search was carried out through PubMed and Embase databases for relevant publications.

Results

Recent calls for investigating suicidality among athletes resulted in a considerable amount of literature providing some evidence regarding lower rates of suicide among professional and high‐performance athletes as well as similar incidence and prevalence of mental conditions, which are known as risk factors for suicide. Nevertheless, special attention is required in this population as predisposing and precipitating factors might differ from classical features of suicidality in the general population. Sports physicians, sports psychiatrists, and other mental health professionals in elite sports should be aware of early signs of affective disorders, risk of recreational drug abuse, misuse of performance‐enhancing medications, sport‐specific environmental stressors, serious physical injuries, and presence of physical or mental illness, all of which may increase suicidality. Traumatic brain injury (TBI) is with suicide with higher severity correlated with increased risk. Compared to active athletes, former athletes may have higher rates of suicide due to common life stressors occurring after sports retirement.

Conclusions

The findings suggest a multidisciplinary approach to suicidality in elite athletes, the main goal of which should be the reduction of suicide‐related morbidity and mortality. Further research is required to clarify the existing gaps in the current knowledge of the issue. While having lower rates of suicide, athletes share some similar (affective disorders, drug abuse, mental and physical illness) and unique factors (misuse of performance‐enhancing substances, sports‐related stressors, sports injuries, TBI) putting them at risk of suicide during active career and retirement.
Image‐based weed recognition and control: Can it select for crop mimicry?
Authors: Coleman Guy R. Y. , Bender Asher , Walsh Michael J. , Neve Paul
Published: January 2023

As highly adaptable plants, weeds have evolved numerous mechanisms to evade control in agroecosystems. For example, reliance on herbicides has resulted in widespread evolution of resistance in many species. Minimising weed adaptation is a major driver for integrated weed management strategies. Crop mimicry is a notable example of weed adaptation, where weed species evolve to avoid control by mimicking aspects of the crop phenotype. Visual selection by hand weeding has been documented to select for crop mimics that are difficult to distinguish from the crop at the vegetative stage. With recent advancements in weed recognition technologies, image‐based weed recognition for in‐crop, site‐specific weed control is on the cusp of becoming widely adopted. Whilst the control methods used in site‐specific weed control will be varied (e.g., spot spraying or lasers), they will share weed recognition technology. Visual selection via image‐based deep learning represents a selection pressure for weeds that can evade detection by mimicking crops. This mimicry may reduce weed recognition accuracy and thus weed control efficacy over time and result in difficult to manage mimetic weed phenotypes. Therefore, it is timely to explore the potential for selection of crop mimics by image‐based weed recognition algorithms.
Invasive African boneseed and a native shrub support similar faunal assemblages in a heavily infested landscape
Authors: Carlos Emma H. , Weston Michael A. , Gibson Maria , Rendall Anthony R.
Published: January 2023

Invasive weeds may provide habitat for wildlife in altered landscapes. We compare fauna within three types of sites in You Yangs Regional Park in central Victoria, Australia, that is, sites with an invasive shrub understory (African boneseed, Chrysanthemoides monilifera subsp. monilifera (DC.) T. Norl.), native shrub understory (snowy mintbush Prostanthera nivea), and sites without an understory (open sites). Sites with an understory dominated by boneseed provided habitat for avian species and an introduced small mammal, comparable with sites with mintbush as the understory. Species‐specific models of common species suggest vegetation structure, rather than composition, drove this effect. Open sites differed in terms of avifaunal assemblage, and the abundance of three common native birds and one common invasive small mammal. Boneseed additionally showed phenological differences in fruiting and flowering and differences in flowering abundance when compared with mintbush, comparably offering resources to wildlife. Transitioning vegetation communities to more natural states should consider wildlife already depending on weeds, a dependency likely driven by a paucity of alternative native vegetation.
Positive and Negative Politeness in Nonverbal Communication Contexts: An Examination into Driving Behaviors in Europe
Authors: AlAfnan Mohammad Awad , MohdZuki Siti Fatimah
Published: January 2023

This study examines politeness as a nonverbal politic behavior in 29 European countries and territories using Brown and Levinson’s (1987) politeness theory. The examination looked into the nonverbal acts of ‘following road signs and speed limit’ and ‘giving signals before changing lanes’ as they reflect (1) negative politeness in the form of avoiding imposition and reducing face-threatening-acts and (2) positive politeness in the form of noticing and appreciating other drivers’ wants and interests to drive without imposition respectively. The examination looked into four variables that are the gender of drivers, age of drivers, level of education of drivers and their country of origin. The study found that European drivers are generally polite and they tend to use the positive politeness act (giving signals) of noticing and appreciating other drivers more often than the negative politeness act (following sigs and speed limits) of reducing imposition and avoiding face-threatening acts. The study also found that the level of education did not play a significant role in the adherence to the driving politic behavior. However, this study found a positive coloration between the age of drivers and their adherence to the driving politic behavior as older drivers follow the driving politic behavior more often than young drivers. This study also found that female drivers in Europe are more polite than male drivers as they notice and appreciate other drivers (positive politeness). This study also revealed that Danish (female), British (male and female), Irish (male), Finish (male), Dutch (male and female), Belgium (female), Austrian (female), Greek (female) and Spanish (male)  drivers have the highest frequency of adherence to the driving politic behavior. The study also found that Portuguese male and female, Croatian male and female, Macedonian male and female, Scottish male and female, Albanian male, Norwegian male, Czech male, Austrian female, Romanian male and Swedish female drivers have the lowest frequency of adherence to the driving politic behavior in Europe. The study also found that Cypriot (male), Catalonian (female), French (male and female), German (male and female), Hungarian (male and female), Italian (male and female), Polish (male and female), Russian (male and female), Serbian (male and female), Slovakian (male and female), and Turkish (male and female) drivers’ adherence to driving politic behavior is relatively moderate to high.
Energy metabolism and muscle activation heterogeneity explain V̇O2${\dot{V}}_{{{\rm{O}}}_{\rm{2}}}$ slow component and muscle fatigue of cycling at different intensities
Authors: do Nascimento Salvador Paulo Cesar , Nascimento Eduardo Marcel Fernandes , Antunes Diego , Guglielmo Luiz Guilherme Antonacci , Denadai Benedito Sérgio
Published: January 2023

This study aimed to explain the slow component () and muscle fatigue during cycling at different intensities. The muscle fatigue of 16 participants was determined through maximal isokinetic effort lasting 3 s during constant work rate bouts of moderate (MOD), heavy (HVY) and very heavy intensity (VHI) exercise. Breath‐by‐breath , near‐infrared spectroscopy signals and EMG activity were analysed (thigh muscles). was higher during VHI exercise (∼70% vs. ∼28% of reserve in HVY). The deoxygenated haemoglobin final value during VHI exercise was higher than during HVY and MOD exercise (∼90% of HHb physiological normalization, vs. ∼82% HVY and ∼45% MOD). The muscle fatigue was greater after VHI exercise (∼22% vs. HVY ∼5%). There was no muscle fatigue after MOD exercise. The greatest magnitude of muscle fatigue occurred within 2 min (VHI ∼17%; HVY ∼9%), after which it stabilized. No significant relationship between and muscle force production was observed. The τ of muscle was significantly related (R2 = 0.47) with torque decrease for VHI. Type I and II muscle fibre recruitment mainly in the rectus femoris moderately explained the muscle fatigue (R2 = 0.30 and 0.31, respectively) and the (R2 = 0.39 and 0.27, respectively). The is also partially explained by blood lactate accumulation (R2 = 0.42). In conclusion muscle fatigue and O2 cost seem to share the same physiological cause linked with a decrease in the muscle and a change in lactate accumulation. Muscle fatigue and are associated with muscle activation heterogeneity and metabolism of different muscles activated during cycling.
Layer‐specific impairment in the developing lateral entorhinal cortex of immune‐challenged Disc1+/− mice
Authors: Kringel Rebecca , Song Lingzhen , Xu Xiaxia , Bitzenhofer Sebastian H. , Hanganu‐Opatz Ileana L.
Published: January 2023

Cognitive deficits in mental disorders result from dysfunctional activity in large‐scale brain networks centred around the hippocampus and the prefrontal cortex. Dysfunctional activity emerges early during development and precedes the cognitive disabilities. The prefrontal–hippocampal network is driven by a prominent input from the lateral entorhinal cortex. We have previously shown that during early development, the entorhinal drive of the prefrontal–hippocampal network is impaired in a mouse model of mental disorders, yet the cellular substrate of this impairment is still poorly understood. Here, we address this question by a detailed characterization of projection neurons across the layers of the lateral entorhinal cortex in immune‐challenged Disc1+/− mice at the beginning of the second postnatal week. We found that the activity and morphology of neurons in layers 2b and 3, which project to the hippocampus, are impaired. Neurons in layer 2b show increased spike‐frequency adaptation, whereas neurons in layer 3 have reduced dendritic complexity but increased spike density. These findings identify the developmental alterations of entorhinal–hippocampal communication that underlie network dysfunction in immune‐challenged Disc1+/− mice.

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Key points

Neonatal immune‐challenged Disc1+/− mice show layer‐specific changes in the lateral entorhinal cortex.

Entorhinal layer 2b pyramidal neurons have increased spike‐frequency adaptation.

Reduced dendritic complexity but increased spine density characterize layer 3 pyramidal neurons.
Expansion of Sandwich Tubes With Metal Foam Core Under Axial Compression
Authors: Guo Haoyuan , Zhang Jianxun
Published: January 2023

In order to enhance the load-carrying and energy-absorbing capacity of the energy-absorbing structure, a new energy-absorbing mode of a metal foam-filled sandwich circular tube (MFSC-Tube) expanded by a conical–cylindrical die under axial compression is designed. An analytical model of expansion of the MFSC-Tube under axial compression is established, considering the bending, stretching, and strain hardening of inner and outer tubes as well as compression of the metal foam core. Subsequently, finite element calculations are performed. Both analytical solutions and deformation modes agree well with the finite element calculation results, which proves the validity of the analytical model. The effects of material and geometrical parameters on the expansion of MFSC-Tubes are considered in detail based on the proposed analytical model. It is found that the load-carrying and energy-absorbing capacity of the expansion of the MFSC-Tube can be enhanced by adjusting such parameters as the ratio of the foam thickness to the wall thickness of the metal circular tube and the semi-angle of the die. Last but not least, the specific energy absorption (SEA) of the MFSC-Tube under expansion is significantly improved compared with that of hollow tubes under expansion.
Calibration of Cellular Automaton Model for Microstructure Prediction in Additive Manufacturing Using Dissimilarity Score
Authors: Ghumman Umar Farooq , Fang Lichao , Wagner Gregory J. , Chen Wei
Published: January 2023

Additive manufacturing (AM) simulations offer an alternative to expensive AM experiments to study the effects of processing conditions on granular microstructures. Existing AM simulations lack support from reliable validation techniques. The stochastic nature and spatial heterogeneity of microstructures make it difficult to validate the simulated microstructures against experimentally obtained images through statistical measures such as average grain size. Another challenge is the lack of reliable and automated methods to calibrate the model parameters, which are unknown and difficult to measure directly from experiments. To overcome these two challenges, we first present a novel metric to quantify the difference between granular microstructures. Then, using this metric in conjunction with Bayesian optimization, we present a framework that can be used to reliably and efficiently calibrate the model parameters. We employ this framework to first calibrate the substrate microstructure simulation and then the laser scan microstructure simulation for Inconel 625. Results show that the framework allows successful calibration of the model parameters in just a small number of simulations.
When Sports Fans Buy: Contextualizing Social Media Engagement Behavior to Predict Purchase Intention
Authors: Steiner Emil , Pittman Matthew , Boatwright Brandon
Published: January 2023

While sports fandom and social media advertising have been widely studied, and all major, professional teams use social media campaigns for direct sales, there is surprisingly little research on the relationship between fans’ social media engagement behavior (SMEB) and their purchase intention (PI), and none that differentiates PI across different platforms and sports contexts. This study addresses those gaps by exploring (a) how different kinds of fans engage their teams’ advertising on various social media and (b) how those different behaviors predict PI in different contexts. To do so, we utilized an SMEB framework to interpret survey data (N = 452) of U.S. sports fans’ social media engagement with their favorite teams over six popular platforms for two situations—in-game and out-of-game. Regression analyses determined the extent to which those behaviors predict PI across different sports and platforms during and outside of games. Our results show that fan SMEB varies by sport, platform, and situation. Furthermore, we found that information-acquiring social media behaviors—such as checking scores—best predict PI in-game, while fan-identity cultivation social media behaviors—such as posting—best predict PI out-of-game. In addition, PI predictability varies across platform and game situation, but not across age, gender, or even level of fandom. By contextualizing the relationship between fan SMEB and PI, our study lays a foundation to address these lingering gaps in the sport communication literature while providing actionable insights for teams and brands seeking more effective sales campaigns across an array of social media.
Mechanical Evaluation of the Resistance of Theraband CLX
Authors: Agustín Rodrigo Martín-San , Medina-Mirapeix Francesc , Gacto-Sánchez Mariano , Cánovas-Ambit Germán , Vecchia Aurelio Arenas-Della
Published: January 2023

Context: Calculating the resistance provided by elastic resistance is essential with the aim of adjusting the load and controlling the progression of the exercises in programs using elastic resistance. This study aimed (1) to establish a model of the force elongation for CLX bands; (2) to examine whether these models are altered by different aspects of band elongation: the phase of elongation and shortening (concentric and eccentric phases), the elongation speed, and the initial resting length; and (3) to determine the resistance value for 100% of elongation in each color and to compare it with the values reported by Theraband. Design: Cross‐sectional. Methods: Ten CLX bands of each color were elongated by 2 experienced researchers to establish their elongation force curves in series of 10 repetitions per band using a Smith machine for an anchor, examining whether elongation force models were affected by elongation and shortening phases using one 4-loop CLX band stretched to 100% and at 0.50 m/s, elongation speed stretching two 4-loop CLX bands at 0.50 m/s and at 0.70 m/s, and different starting lengths using 3 CLX bands (2, 3, and 4 loops) stretched at 0.50 m/s. Results: No differences were found in the comparisons between phases, speeds, or different start lengths (mean errors ranged from 0.01 [0.07 N] for the blue band to 2.97 [0.94 N] for the gold band). Our values were higher than the reference values provided by Theraband for all colors, ranging from 2.3% to 33.1%. Conclusions: Our findings show that the values provided by the brand underestimate the resistance provided by CLX bands. To solve this, regression equations are provided so professionals can calculate the resistance of CLX bands based on their elongation. In addition, these models are not influenced by the phase of elongation and shortening, the elongation speed, and the initial resting length.
Tyrosine kinase inhibitors can activate the NLRP3 inflammasome in myeloid cells through lysosomal damage and cell lysis
Authors: Neuwirt Emilia , Magnani Giovanni , Ćiković Tamara , Wöhrle Svenja , Fischer Larissa , Kostina Anna , Flemming Stephan , Fischenich Nora J. , Saller Benedikt S. , Gorka Oliver , Renner Steffen , Agarinis Claudia , Parker Christian N. , Boettcher Andreas , Farady Christopher J. , Kesselring Rebecca , Berlin Christopher , Backofen Rolf , Rodriguez-Franco Marta , Kreutz Clemens , Prinz Marco , Tholen Martina , Reinheckel Thomas , Ott Thomas , Groß Christina J. , Jost Philipp J. , Groß Olaf
Published: January 2023

Inflammasomes are intracellular protein complexes that promote an inflammatory host defense in response to pathogens and damaged or neoplastic tissues and are implicated in inflammatory disorders and therapeutic-induced toxicity. We investigated the mechanisms of activation for inflammasomes nucleated by NOD-like receptor (NLR) proteins. A screen of a small-molecule library revealed that several tyrosine kinase inhibitors (TKIs)—including those that are clinically approved (such as imatinib and crizotinib) or are in clinical trials (such as masitinib)—activated the NLRP3 inflammasome. Furthermore, imatinib and masitinib caused lysosomal swelling and damage independently of their kinase target, leading to cathepsin-mediated destabilization of myeloid cell membranes and, ultimately, cell lysis that was accompanied by potassium (K
+
) efflux, which activated NLRP3. This effect was specific to primary myeloid cells (such as peripheral blood mononuclear cells and mouse bone marrow–derived dendritic cells) and did not occur in other primary cell types or various cell lines. TKI-induced lytic cell death and NLRP3 activation, but not lysosomal damage, were prevented by stabilizing cell membranes. Our findings reveal a potential immunological off-target of some TKIs that may contribute to their clinical efficacy or to their adverse effects.
A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity
Authors: Kim Hyunbae , Song Zhenfeng , Zhang Ren , Davies Brandon S. J. , Zhang Kezhong
Published: January 2023

The endoplasmic reticulum (ER)–tethered, liver-enriched stress sensor CREBH is processed in response to increased energy demands or hepatic stress to release an amino-terminal fragment that functions as a transcription factor for hepatic genes encoding lipid and glucose metabolic factors. Here, we discovered that the carboxyl-terminal fragment of CREBH (CREBH-C) derived from membrane-bound, full-length CREBH was secreted as a hepatokine in response to fasting or hepatic stress. Phosphorylation of CREBH-C mediated by the kinase CaMKII was required for efficient secretion of CREBH-C through exocytosis. Lipoprotein lipase (LPL) mediates the lipolysis of circulating triglycerides for tissue uptake and is inhibited by a complex consisting of angiopoietin-like (ANGPTL) 3 and ANGPTL8. Secreted CREBH-C blocked the formation of ANGPTL3-ANGPTL8 complexes, leading to increased LPL activity in plasma and metabolic tissues in mice. CREBH-C administration promoted plasma triglyceride clearance and partitioning into peripheral tissues and mitigated hypertriglyceridemia and hepatic steatosis in mice fed a high-fat diet. Individuals with obesity had higher circulating amounts of CREBH-C than control individuals, and human
CREBH
loss-of-function variants were associated with dysregulated plasma triglycerides. These results identify a stress-induced, secreted protein fragment derived from CREBH that functions as a hepatokine to stimulate LPL activity and triglyceride homeostasis.