bluuebunny/crossref_metadata_2025 huggingface dataset:
Silk fibroin carriers with sustained release capacity for treating neurological diseases
Authors: Huang Xinqi , An Yumei , Yuan Shengye , Chen Chen , Shan Haiyan , Zhang Mingyang
Published: May 2023

Neurological diseases such as traumatic brain injury, cerebral ischemia, Parkinson’s, and Alzheimer’s disease usually occur in the central and peripheral nervous system and result in nervous dysfunction, such as cognitive impairment and motor dysfunction. Long-term clinical intervention is necessary for neurological diseases where neural stem cell transplantation has made substantial progress. However, many risks remain for cell therapy, such as puncture bleeding, postoperative infection, low transplantation success rate, and tumor formation. Sustained drug delivery, which aims to maintain the desired steady-state drug concentrations in plasma or local injection sites, is considered as a feasible option to help overcome side effects and improve the therapeutic efficiency of drugs on neurological diseases. Natural polymers such as silk fibroin have excellent biocompatibility, which can be prepared for various end-use material formats, such as microsphere, gel, coating/film, scaffold/conduit, microneedle, and enables the dynamic release of loaded drugs to achieve a desired therapeutic response. Sustained-release drug delivery systems are based on the mechanism of diffusion and degradation by altering the structures of silk fibroin and drugs, factors, and cells, which can induce nerve recovery and restore the function of the nervous system in a slow and persistent manner. Based on these desirable properties of silk fibroin as a carrier with sustained-release capacity, this paper discusses the role of various forms of silk fibroin-based drug delivery materials in treating neurological diseases in recent years.
Pyroptosis-related genes prognostic model for predicting targeted therapy and immunotherapy response in soft tissue sarcoma
Authors: Liu Mengmeng , Li Quan , Liang Yao
Published: May 2023

Several studies have highlighted the potential of pyroptosis as a target for cancer treatment. This article focuses on the specific roles and clinical implications of pyroptosis-related genes (PRGs) in soft tissue sarcoma (STS). By analyzing differentially expressed PRGs in STS compared to normal tissue, our study evaluates the interactions, biological functions, and prognostic values of PRGs in STS. Through LASSO COX regression analysis, a five-gene survival related-risk score (PLCG1, PYCARD, CASP8, NOD1, and NOD2) was created, which examined both in TCGA cohort and training cohort (GSE21050, GSE30929, and GSE63157). Furthermore, we developed a nomogram incorporating clinic factors and the risk scores of the PRGs, which showed decent accuracy of prediction as evidenced by calibration curves. Additionally, our study analyzed the Tumor Immune Dysfunction and Exclusion Algorithm (TIDE) and IMvigor 210 cohorts to investigate the immunotherapy response, and found that immunotherapy was more beneficial for patients with minimal risk of PRGs than those exhibiting greater risk. Finally, GDSC and CAMP databases were used to screen for effective chemotherapy or targeted drugs that are sensitive to the high-risk populations, including doxorubicin, imatinib, and sorafenib. In conclusion, this study provides a comprehensive analysis of the PRG landscape in STS and constructs a novel risk model to predict prognosis and different therapeutic responses of STS patients, which is helpful for achieving precision medicine.
A stochastic reaction–diffusion modeling investigation of FLASH ultra-high dose rate response in different tissues
Authors: Abolfath Ramin , Baikalov Alexander , Fraile Alberto , Bartzsch Stefan , Schüler Emil , Mohan Radhe
Published: May 2023

Purpose: The aim of the study was to propose a theory based on topology and geometry of diffusion channels in tissue to contribute to the mechanistic understanding of normal tissue sparing at ultra-high dose rates (UHDRs) and explore an interplay between intra- and inter-track radical recombination through a reaction–diffusion mechanism.

Methods: We calculate the time evolution of particle track structures using a system of coupled reaction–diffusion equations on a random network designed for molecular transport in porous and disordered media. The network is representative of the intra- and inter-cellular diffusion channels in tissues. Spatial cellular heterogeneities over the scale of track spacing are constructed by incorporating random fluctuations in the connectivity between network sites, resembling molecular mass and charge heterogeneities at the cellular level.

Results: We demonstrate the occurrence of phase separation among the tracks as the complexity in intra- and inter-cellular structure increases. At the strong limit of structural disorder, tracks evolve individually like isolated islands with negligible inter-track as they propagate like localized waves in space, analogous to the Anderson localization in quantum mechanics. In contrast, at the limit of weak disorder in a homogeneous medium, such as water, the neighboring tracks melt into each other and form a percolated network of non-reactive species. Thus, the spatiotemporal correlation among chemically active domains vanishes as the inter-cellular complexity of the tissue increases from normal tissue structure to fractal-type malignancy.

Conclusion: Differential FLASH normal tissue sparing may result from the interplay of the proximity of tracks over the intra- and inter-cellular landscape, a transition in the spatial distribution of chemical reactivity, and molecular crowding. In this context, insensitivities in the radiobiological responses of the tumors to FLASH-UHDR are interpreted via a lack of geometrical correlation among isolated tracks. The structural and geometrical complexities of cancerous cells prevent the clustering of tracks over a timescale, in which inter-track chemical reactivities presumably prevail in normal tissues. A series of systematic experiments on radiolysis-induced diffusivity and reactivity in actual normal and cancerous tissues must be performed to classify the tissues potentially spared by FLASH-UHDRs and verify our theory.
Hemispherical lens integrated room temperature ultra-broadband GaAs HEMT terahertz detector
Authors: Yao Chenyu , Jiang Mengjie , Wang Dong , Zhang Libo , Zhang Ning , Wang Lin , Chen Xiaoshuang
Published: May 2023

Terahertz (THz) detection has been extensively studied in the application fields of wireless communication, non-destructive imaging and spectrum inspection, etc., in the decades. Here, we report that a self-mixing terahertz detector based on hemispherical silicon lens and antenna-coupled InGaAs/AlGaAs high electron mobility transistor (HEMT) enables wide spectrum detection, and improves detection performances. In this device configuration, the optimal log-periodic antenna is used to enhance light field in the channel, the double-gate could further adjust the carrier concentration, and the hemispheric silicon lens can concentrate the terahertz radiation in the center of the hemisphere to effectively improve the terahertz wave collection efficiency, which could improve the sensitivity of the detector. The broadband Terahertz responsivity and noise equivalent power (NEP) of the detector are characterized at the range from 0.07 to 0.82 THz at room temperature. A peak responsivity of 2 kV/W and a minimum optical noise equivalent power of 59 pW/Hz1/2 at 0.8 THz without any external field, are reached from two-terminal configuration at 0.8 THz. The minimum optical NEP of the device can be reduced to 30 pW/Hz1/2 by applying a gate voltage, realizing scanning imaging demonstration.
Phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ARDS model
Authors: Bassi Thiago G. , Rohrs Elizabeth C. , Fernandez Karl C. , Ornowska Marlena , Nicholas Michelle , Wittmann Jessica , Gani Matt , Evans Doug , Reynolds Steven C.
Published: May 2023

Rationale: In porcine healthy-lung and moderate acute respiratory distress syndrome (ARDS) models, groups that received phrenic nerve stimulation (PNS) with mechanical ventilation (MV) showed lower hippocampal apoptosis, and microglia and astrocyte percentages than MV alone.

Objectives: Explore whether PNS in combination with MV for 12 h leads to differences in hippocampal and brainstem tissue concentrations of inflammatory and synaptic markers compared to MV-only animals.

Methods: Compare tissue concentrations of inflammatory markers (IL-1α, IL-1β, IL-6, IL-8, IL-10, IFN-γ, TNFα and GM-CSF), pre-synaptic markers (synapsin and synaptophysin) and post-synaptic markers (disc-large-homolog 4, N-methyl-D-aspartate receptors 2A and 2B) in the hippocampus and brainstem in three groups of mechanically ventilated pigs with injured lungs: MV only (MV), MV plus PNS every other breath (MV + PNS50%), and MV plus PNS every breath (MV + PNS100%). MV settings in volume control were tidal volume 8 ml/kg, and positive end-expiratory pressure 5 cmH2O. Moderate ARDS was achieved by infusing oleic acid into the pulmonary artery.

Measurements and Main Results: Hippocampal concentrations of GM-CSF, N-methyl-D-aspartate receptor 2B, and synaptophysin were greater in the MV + PNS100% group compared to the MV group, p = 0.0199, p = 0.0175, and p = 0.0479, respectively. The MV + PNS100% group had lower brainstem concentrations of IL-1β, and IL-8 than the MV group, p = 0.0194, and p = 0.0319, respectively; and greater brainstem concentrations of IFN-γ and N-methyl-D-aspartate receptor 2A than the MV group, p = 0.0329, and p = 0.0125, respectively.

Conclusion: In a moderate-ARDS porcine model, MV is associated with hippocampal and brainstem inflammation, and phrenic nerve stimulation on every breath mitigates that inflammation.
Employee human resource management values: validation of a new concept and scale
Authors: Drouin-Rousseau Sophie , Fernet Claude , Austin Stéphanie , Fabi Bruno , Morin Alexandre J. S.
Published: May 2023

Purpose

Although human resource management (HRM) practices all seek to support and improve organizational functioning, the value ascribed to various HRM practices differs greatly among employees. Drawing on an exhaustive measure of HRM practices, this study proposed a new conceptualization and measure of HRM values, the HRM Values Scale (HRM-VS).

Design/methodology/approach

To examine the psychometric properties of scores obtained on this new measure, we rely on a sample of 979 employees occupying a variety of jobs within various private and public organizations.

Findings

Through the comparison of confirmatory factor analysis (CFA) and exploratory structural equation modeling (ESEM) solutions, our results supported a nine-factor structure of participants’ responses to the HRM-VS and the measurement invariance of this solution across male and female employees. Specifically, they support that the HRM-VS items adequately capture core HRM values underlying independent HRM practices. Criterion-related validity was evidenced with respect to employees’ ratings of intrinsic and extrinsic job satisfaction.

Research implications

The HRM-VS appears to represent a promising tool for research and intervention seeking to account for individual differences in the relative importance of various HRM practices, in order to devise more effective HRM systems.

Practical implications

This new concise but complete measure could help better guide organizations in tailoring their strategic HRM.

Originality/value

This study introduces HRM values as a valid concept that characterizes what employees desire or consider to be important in relation to HRM practices.
Building community through higher music education: a training program for facilitating musical engagement among older adults
Authors: Paolantonio Paolo , Cavalli Stefano , Biasutti Michele , Eiholzer Hubert , Williamon Aaron
Published: May 2023

Preparing music students to design and carry out community-based initiatives can be an effective way to enhance their employability and their wellbeing. With a large body of evidence now pointing to the benefits of musical engagement for older adults, both for individuals and for society as a whole, there is considerable opportunity and value in training aspiring professional musicians to work with and on behalf of those in their third and fourth age. This article describes a seminar designed by a Swiss conservatoire in collaboration with local nursing homes involving residents and music university students in a 10-week group music making program. On the basis of the positive results to emerge in terms of health, wellbeing and career preparation, we aim to provide information relevant for colleagues to replicate this seminar in other higher music education institutions. Moreover, this paper aims to shed light on the complexity of designing music students’ training so that they acquire the competences needed to deliver meaningful, community-based initiatives alongside their other professional training commitments, and to provide directions for future research. The development and implementation of these points could foster the increase and sustainability of innovative programs beneficial for older adults, musicians and local communities.
How to improve pedestrians' trust in automated vehicles: new road infrastructure, external human–machine interface with anthropomorphism, or conventional road signaling?
Authors: Bonneviot Flavie , Coeugnet Stéphanie , Brangier Eric
Published: May 2023

Introduction

Automated vehicles need to gain the trust of all road users in order to be accepted. To make technology trustworthy, automated vehicles must transmit crucial information to pedestrians through a human-machine interface, allowing pedestrians to accurately predict and act on their next behavior. However, the unsolved core issue in the field of vehicle automation is to know how to successfully communicate with pedestrians in a way that is efficient, comfortable, and easy to understand. This study investigated the impact of three human-machine interfaces specifically designed for pedestrians' trust during the street crossing in front of an automated vehicle. The interfaces used different communication channels to interact with pedestrians, i.e., through a new road infrastructure, an external human-machine interface with anthropomorphism, or with conventional road signaling.

Methods

Mentally projected in standard and non-standard use cases of human-machine interfaces, 731 participants reported their feelings and behavior through an online survey.

Results

Results showed that human-machine interfaces were efficient to improve trust and willingness to cross the street in front of automated vehicles. Among external human-machine interfaces, anthropomorphic features showed significant advantages in comparison with conventional road signals to induce pedestrians' trust and safer crossing behaviors. More than the external human-machine interfaces, findings highlighted the efficiency of the trust-based road infrastructure on the global street crossing experience of pedestrians with automated vehicles.

Discussion

All of these findings support trust-centered design to anticipate and build safe and satisfying human-machine interactions.
Shared book reading as a context for language intervention for children with Down syndrome: a mini-review
Authors: Jeremic Mirjana , Stojanovik Vesna , Burgoyne Kelly , Pagnamenta Emma
Published: May 2023

Acquiring language and communication skills is one of the biggest challenges for children with Down syndrome (DS). However, few evidence-based interventions exist to enhance the development of language and communication in this population. Shared book reading (SBR) is well-established as an effective intervention for language and communication development of typically developing children, and evidence of the possible effectiveness of this approach for those at risk of language difficulties is emerging. This paper provides a mini-review of the existing evidence for SBR in relation to language and communication outcomes for young children with DS. A systematic literature search was conducted with the following inclusion criteria: children with DS aged 0–6;11 years, SBR, language or communication outcomes. The results show that interventions which incorporate SBR strategies are associated with improved language and communication outcomes for young children with DS, improved parental sensitivity, and continuing implementation of SBR strategies following intervention instruction. However, evidence is limited in scope, of low quality, including mostly single case studies, with only one study having a control group. We conclude that although SBR may hold promise as a possible intervention, further research is essential to establish what specific components of SBR intervention are most effective for young children with DS and what further adaptations are needed to accommodate the cognitive profile and variability within this population.
Momentary engagement in simultaneous versus consecutive interpreting: through the lens of translanguaging and CDST
Authors: Han Lili , Lu Jing , (Edward) Wen Zhisheng , Tian Yuan
Published: May 2023

Through the lens of translanguaging theory and the complex, dynamic system theory (CDST) approach, the interpreting process is considered a highly complex and dynamic activity that engages the interpreter’s cognition, emotion, and action during successive “translanguaging moments” of meaning-making. Meanwhile, the two dominant types of interpreting, namely, simultaneous interpreting, and consecutive interpreting are assumed to entail distinct time sensitivity and consume different amounts of cognitive resources at different stages. Based on these assumptions, the present study analyzes interpreters’ momentary engagement during the distinct workflow tasks associated with these two modes of interpreting, with a view to probing their underlying non-linearity, self-organization and emergence dynamics from a micro-level perspective. Furthermore, we triangulated the textual description with multimodal transcription to portray these “translanguaging moments,” which are augmented with a follow-up emotional survey that corroborated our findings.
High treatment resistance is associated with lower performance in the Stroop test in patients with obsessive–compulsive disorder
Authors: Doolub Damien , Vibert Nicolas , Botta Fabiano , Razmkon Ali , Bouquet Cédric , Wassouf Issa , Millet Bruno , Harika-Germaneau Ghina , Jaafari Nematollah
Published: May 2023

Around 50% of the patients with obsessive–compulsive disorder (OCD) are resistant to treatment, and patients with OCD show alterations in a broad range of cognitive abilities. The present study investigated the links between treatment-resistance, executive and working memory abilities, and the severity of OCD symptoms among 66 patients with OCD. The patients performed seven tests gauging their executive functions and working memory and filled in questionnaires for OCD severity and insight into their pathology. In addition, the executive and working memory abilities of a subset of these patients were compared with those of individually matched control participants. In contrast with previous studies, patients’ treatment resistance was evaluated by considering the clinical outcomes of all the treatments that they received during the course of their disease. Higher treatment resistance was associated with lower performance in one particular executive test, the Stroop test, which assessed patients’ ability to inhibit prepotent/automatic responses. Older age and more severe OCD symptoms were also associated with higher treatment resistance. Regardless of OCD severity, the patients displayed small to moderate deficits across most components of executive functions compared to control participants. Interestingly, patients with OCD took more time than control participants to perform speeded neuropsychological tests but never made more errors. Altogether, this study shows that the treatment-resistance of patients with OCD may be reliably quantified over the course of years and treatments using Pallanti and Quercioli’s (2006) treatment resistance-related scales. The data suggest that the Stroop test could be used clinically to anticipate treatment outcomes in to-be-treated patients.
The well-being of Iranian adult citizens; is it related to mental health literacy?
Authors: Mahmoodi Seyed Mohammad Hossein , Rasoulian Maryam , Khodadoust Elaheh , Jabari Zahra , Emami Sahar , Ahmadzad-Asl Masoud
Published: May 2023

Background

Subjective well-being (SWB) is a fundamental concept in the definition of mental health and is a significant health indicator for individuals and societies. Mental health literacy (MHL) is a modifiable variable with known effects on mental health, but its relationship with SWB is not recognized. In this study, the SWB is measured, and its relationship to MHL is investigated.

Methods

In this cross-sectional study conducted in Iran in 2019, 1,682 individuals participated using a convenient sampling method. Participants with a basic ability to use internet were included. A simple online form was used to collect data. SWB and MHL were measured with three questionnaires: WHO-5 Well-Being Index, Mental Health Literacy Scale, and Mental Health Positive Knowledge.

Results

Most of the participants were young (mean age 25.99, SD 9.14), female (71.9%), and had a university degree (78.5%). The mean SWB was 50.19 out of 100 (SD 20.92). More than half of the participants (50.4%) were screen-positive for clinical depression regarding their low well-being. Significant but very small correlations were detected between SWB and both MHL measures.

Conclusion

The well-being of half of the educated Iranian citizens who participated in this study was poor and lower than previous measurements. No strong correlation is detected between SWB and MHL measures in this study. This suggests that people’s well-being cannot be improved by merely implementing mental health educational programs.
The expansion of newborn neurons in hippocampus improves social recognition deficit in a mouse model of autism
Authors: Meng Hu , Li Qiongwei , Wang Jinxin , Yue Weihua , Zhang Dai , Sun Xiaoxuan , Wang Lifang , Li Jun
Published: May 2023

Introduction

Autism spectrum disorders (ASDs) are a group of neurodevelopmental disorders characterized by core symptoms of impaired social interaction and communication. The pathological mechanism and treatment are not clear and need further study. Our previous study found that the deletion of high-risk gene Autism Susceptibility 2 (AUTS2) in mice led to dentate gyrus (DG) hypoplasia that highly associated with impaired social novelty recognition. Here we aim to improve the social deficit through increasing the neurogenesis in the subgranular zone (SGZ) and expanding the newborn granule neurons in DG.

Methods

Three approaches including repeated oxytocin administration, feeding in enriched environment and overexpression of cyclin-dependent kinase 4 (Cdk4)-CyclinD1 complex in DG neural stem cells (NSCs) at the post-weaning stage were conducted.

Results

We found that the number of EdU labeled proliferative NSCs or retrovirus labeled newborn neurons was significantly increased after manipulations. The social recognition deficit was also significantly improved.

Discussion

Our findings suggested a possible strategy to restore the social deficit through expansion of newborn neurons in hippocampus, which might provide a new insight into the treatment of autism.
Local initiative supports case isolation and contact tracing during a SARS-CoV-2 surge in summer 2020: a community case study in Antwerp, Belgium
Authors: Verdonck Kristien , Morreel Stefan , Vanhamel Jef , Vuylsteke Bea , Nöstlinger Christiana , Laga Marie , van Olmen Josefien
Published: May 2023

In Antwerp, Belgium's second largest city, a COVID-19 surge in July 2020 predominantly affected neighborhoods with high ethnic diversity. Local volunteers reacted and set up an initiative to support contact tracing and self-isolation. We describe the origin, implementation, and transfer of this local initiative, based on semi-structured interviews of five key informants and document review. The initiative started in July 2020, when family physicians signaled a surge of SARS-CoV-2 infections among people of Moroccan descent. Family physicians feared that the mainstream contact tracing organized by the Flemish government through centralized call centers would not be efficient in halting this outbreak. They anticipated language barriers, mistrust, inability to investigate case clusters, and practical problems with self-isolation. It took 11 days to start up the initiative, with logistical support from the province and city of Antwerp. Family physicians referred SARS-CoV-2-infected index cases with complex needs (including language and social situation) to the initiative. Volunteer COVID coaches contacted cases, got a contextualized understanding of their living conditions, assisted with backward and forward contact tracing, offered support during self-isolation, and checked if infected contacts also needed support. Interviewed coaches were positive about the quality of the interaction: they described extensive open conversations with cases. The coaches reported back to referring family physicians and coordinators of the local initiative, who took additional action if necessary. Although interactions with affected communities were perceived as good, respondents considered that the number of referrals by family physicians was too low to have a meaningful impact on the outbreak. In September 2020, the Flemish government assigned the tasks of local contact tracing and case support to the local health system level (primary care zones). While doing so, they adopted elements of this local initiative, such as COVID coaches, tracing system, and extended questionnaires to talk with cases and contacts. This community case study illustrates how urgency can motivate people to action yet support from people with access to resources and coordination capacity is vital for effective organization and transition to long-term sustainability. From their conception, health policies should consider adaptability of new interventions to local contexts.
Epidemiological and demographic drivers of lung cancer mortality from 1990 to 2019: results from the global burden of disease study 2019
Authors: Fan Yaguang , Jiang Yong , Gong Lei , Wang Ying , Su Zheng , Li Xuebing , Wu Heng , Pan Hongli , Wang Jing , Meng Zhaowei , Zhou Qinghua , Qiao Youlin
Published: May 2023

Background

Understanding the effects of demographic drivers on lung cancer mortality trends is critical for lung cancer control. We have examined the drivers of lung cancer mortality at the global, regional, and national levels.

Methods

Data on lung cancer death and mortality were extracted from the Global Burden of Disease (GBD) 2019. Estimated annual percentage change (EAPC) in the age-standardized mortality rate (ASMR) for lung cancer and all-cause mortality were calculated to measure temporal trends in lung cancer from 1990 to 2019. Decomposition analysis was used to analyze the contributions of epidemiological and demographic drivers to lung cancer mortality.

Results

Despite a non-significant decrease in ASMR [EAPC = −0.31, 95% confidence interval (CI): −1.1 to 0.49], the number of deaths from lung cancer increased by 91.8% [95% uncertainty interval (UI): 74.5–109.0%] between 1990 and 2019. This increase was due to the changes in the number of deaths attributable to population aging (59.6%), population growth (56.7%), and non-GBD risks (3.49%) compared with 1990 data. Conversely, the number of lung cancer deaths due to GBD risks decreased by 19.8%, mainly due to tobacco (−12.66%), occupational risks (−3.52%), and air pollution (−3.47%). More lung cancer deaths (1.83%) were observed in most regions, which were due to high fasting plasma glucose levels. The temporal trend of lung cancer ASMR and the patterns of demographic drivers varied by region and gender. Significant associations were observed between the contributions of population growth, GBD risks and non-GBD risks (negative), population aging (positive), and ASMR in 1990, the sociodemographic index (SDI), and the human development index (HDI) in 2019.

Conclusion

Population aging and population growth increased global lung cancer deaths from 1990 to 2019, despite a decrease in age-specific lung cancer death rates due to GBD risks in most regions. A tailored strategy is needed to reduce the increasing burden of lung cancer due to outpacing demographic drivers of epidemiological change globally and in most regions, taking into account region- or gender-specific risk patterns.
Detection of Burkholderia pseudomallei with CRISPR-Cas12a based on specific sequence tags
Authors: Zhang Jia-Xin , Xu Jian-Hao , Yuan Bing , Wang Xiao-Dong , Mao Xu-hu , Wang Jing-Lin , Zhang Xiang-Li-Lan , Yuan Yuan
Published: May 2023

Melioidosis is a bacterial infection caused by Burkholderia pseudomallei (B. pseudomallei), posing a significant threat to public health. Rapid and accurate detection of B. pseudomallei is crucial for preventing and controlling melioidosis. However, identifying B. pseudomallei is challenging due to its high similarity to other species in the same genus. To address this issue, this study proposed a dual-target method that can specifically identify B. pseudomallei in less than 40 min. We analyzed 1722 B. pseudomallei genomes to construct large-scale pan-genomes and selected specific sequence tags in their core genomes that effectively distinguish B. pseudomallei from its closely related species. Specifically, we selected two specific tags, LC1 and LC2, which we combined with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated proteins (Cas12a) system and recombinase polymerase amplification (RPA) pre-amplification. Our analysis showed that the dual-target RPA-CRISPR/Cas12a assay has a sensitivity of approximately 0.2 copies/reaction and 10 fg genomic DNA for LC1, and 2 copies/reaction and 20 fg genomic DNA for LC2. Additionally, our method can accurately and rapidly detect B. pseudomallei in human blood and moist soil samples using the specific sequence tags mentioned above. In conclusion, the dual-target RPA-CRISPR/Cas12a method is a valuable tool for the rapid and accurate identification of B. pseudomallei in clinical and environmental samples, aiding in the prevention and control of melioidosis.
A conceptual framework for selecting appropriate populations for public health interventions
Authors: Aagaard-Hansen Jens , Hindhede Anette Lykke , Terkildsen Maindal Helle
Published: May 2023

This article suggests a conceptual framework for choice of target populations for public health interventions. In short, who should benefit? Taking the seminal work of Geoffrey Rose on “individuals at risk” versus the “whole population approach” as a point of departure, we explore later contributions. Frohlich and Potvin introduced the notion of “vulnerable populations” applying relevant social determinants as the defining selection criterion. Other interventions focus on a “physical space” (spatial demarcations) such as a neighborhood as a means to define intervention populations. As an addition to these criteria, we suggest that the life-course perspective entails an alternative means of selecting target populations based on a “temporal” perspective. A focus on the various age phases ranging from fetal life and infancy to old age may guide selection of population segments for targeted public health interventions. Each of the selection criteria has advantages and disadvantages when used for primary, secondary, or tertiary prevention. Thus, the conceptual framework may guide informed decisions in public health planning and research regarding precision prevention versus various approaches to complex community-based interventions.
PRPF19 modulates morphology and growth behavior in a cell culture model of human skin
Authors: Kleissl Lisa , Weinmüllner Regina , Lämmermann Ingo , Dingelmaier-Hovorka Ruth , Jafarmadar Mohammad , El Ghalbzouri Abdoelwaheb , Stary Georg , Grillari Johannes , Dellago Hanna
Published: May 2023

The skin provides one of the most visual aging transformations in humans, and premature aging as a consequence of oxidative stress and DNA damage is a frequently seen effect. Cells of the human skin are continuously exposed to endogenous and exogenous DNA damaging factors, which can cause DNA damage in all phases of the cell cycle. Increased levels of DNA damage and/or defective DNA repair can, therefore, accelerate the aging process and/or lead to age-related diseases like cancer. It is not yet clear if enhanced activity of DNA repair factors could increase the life or health span of human skin cells. In previous studies, we identified and characterized the human senescence evasion factor (SNEV)/pre-mRNA-processing factor (PRPF) 19 as a multitalented protein involved in mRNA splicing, DNA repair pathways and lifespan regulation. Here, we show that overexpression of PRPF19 in human dermal fibroblasts leads to a morphological change, reminiscent of juvenile, papillary fibroblasts, despite simultaneous expression of senescence markers. Moreover, conditioned media of this subpopulation showed a positive effect on keratinocyte repopulation of wounded areas. Taken together, these findings indicate that PRPF19 promotes cell viability and slows down the aging process in human skin.
Sanjiangyuan Eco-immigrants: lifestyle, social adaptation, change of idea and urbanization process
Authors: Shi Desheng , Yang Zicong
Published: May 2023

This paper describe the lifestyle about Eco-immigrants after relocated into city during the implementation of Sanjiangyuan ecological protection plans, and study the social adaptation and change of ideas about Eco-immigrants in the rapid urbanization. Then the author thinks that the lifestyle of immigrants presents heavy traditional characteristics because lack of prominent pluralism, little social mobility, low occupational differentiation, and heterogeneity. At same time, Eco-immigrants experience cultural oscillation and forms marginal personality in social and culture adaptation process, due to the slow process of rationalization, secularization, without forming a stable and complete market economy and urban lifestyle, urbanization of Eco-immigrants is at the primary stage.
RIS-aided integrated sensing and communication: a mini-review
Authors: Asif Haider Mirza , Zhang Yimin D.
Published: May 2023

Integrating sensing and communication (ISAC) is a cutting-edge technology aimed at achieving high-resolution target sensing and high data-rate communications using a shared spectrum. This innovative approach optimizes the usage of the radio spectrum with no or minimal level of mutual interference. The capability of reconfigurable intelligent surface (RIS) to control the environment and provide additional degrees of freedom is driving the development of RIS-aided ISAC. In this mini-review, we provide an overview of the current state-of-the-art of RIS-aided ISAC technology, including various system configurations, approaches, and signal processing techniques.
Influence of water quality on benthic macroinvertebrates in a groundwater-dependent wetland
Authors: Banda Kawawa , Ngwenya Victoria , Mulema Mataa , Chomba Innocent , Chomba Machaya , Nyambe Imasiku
Published: May 2023

Benthic invertebrates communities are frequently used as indicators of aquatic ecosystem health since many species are sensitive to pollution and abrupt changes in their environment. Limited knowledge exists on the interlinkages of hydrological dynamics, water quality and the ecological character of groundwater-dependant ecosystems especially in developing countries. In this study we assessed the sensitivity of benthic macroinvertebrates to water quality dynamics in the Barotse Floodplain, a groundwater-dependant wetland. Benthic invertebrates were sampled in the dry season using the kick-net method at selected points upstream, mid-stream and downstream. The selection of sampled points was based on an initially conducted water quality survey that characterized the wetland into mainly two water types, NaHCO3 (upstream) and CaMgHCO3 (downstream). Canonical Correspondence Analysis (CCA) was used to investigate the influence of water quality on macroinvertebrate subclass-taxa level. Furthermore, factor analysis was used to derive the processes propagating the observed water quality variability. It was established that the composition and diversity of macroinverbrate communities at subclass-taxa level was influenced by effects of the wetland flood pulse, salinity (mineralisation) from groundwater input and biogeochemical processes during the expansion and contraction of the floodplain-river exchange. This study has demonstrated that biomonitoring was effective in capturing the natural processes/regimes of the environmental (such as flooding) and thus has potential to be used for mointoring extreme effects of phenomenon such as climate change. It is recommended that, the families, genus and species taxonomic levels are needed to improve the understanding of responses of the subclass-taxa level and the detection of specific contamination signatures, to ensure wetland conservation and protection. Integrated water resources management for wetlands thus should incorporate biomonitoring conjuctively with traditional methods to ensure vital ecosystems are not compromised at the expense of maximizing the economic and social welfare of humanity.
Risk factors for intimate partner emotional violence among women in union in Uganda
Authors: Nakitto Resty , Nzabona Abel , Wandera Stephen Ojiambo
Published: May 2023

Introduction

Despite the growing evidence of the prevalence of gender-based violence in Uganda, less is known about the factors influencing intimate partner emotional violence (IPEV) among married women in the country. This study investigated the social demographic factors associated with IPEV among married women aged 15 years and older.

Data and methods

The study used the 2016 Uganda Demographic Healthy Survey (UDHS) data. A weighted sample of 5,642 women who had been in a union was selected. A binary logistic regression model was fitted to analyze the predictors of IPEV.

Results

Almost four in 10 (38%) married women experienced IPEV. Witnessing parental violence (OR = 1.37, CI = 0.59–0.92), partner's controlling behavior (OR = 4.26, CI = 3.29–5.52), and attaining age 35+ (OR = 1.44, CI = 1.06–1.95) increased the odds of IPEV. Residing in rural areas (OR = 0.004, CI = 0.48–0.99) and having higher education (OR = 0.51, CI = 0.26–1.00) decreased the odds of IPEV.

Conclusion and implications

Witnessing parental violence, alcohol consumption, age, place of residence, partner's controlling behavior, and level of education influence IPEV among married women in Uganda. The findings have several implications including strengthening IPEV-prevention campaigns, women empowerment, and alcohol consumption regulations.
Immediate effects of acute Mars mission equivalent doses of SEP and GCR radiation on the murine gastrointestinal system-protective effects of curcumin-loaded nanolipoprotein particles (cNLPs)
Authors: Diaz Jonathan , Kuhlman Bradford M. , Edenhoffer Nicholas P. , Evans Angela C. , Martin Kelly A. , Guida Peter , Rusek Adam , Atala Anthony , Coleman Matthew A. , Wilson Paul F. , Almeida-Porada Graça , Porada Christopher D.
Published: May 2023

Introduction: Missions beyond low Earth orbit (LEO) will expose astronauts to ionizing radiation (IR) in the form of solar energetic particles (SEP) and galactic cosmic rays (GCR) including high atomic number and energy (HZE) nuclei. The gastrointestinal (GI) system is documented to be highly radiosensitive with even relatively low dose IR exposures capable of inducing mucosal lesions and disrupting epithelial barrier function. IR is also an established risk factor for colorectal cancer (CRC) with several studies examining long-term GI effects of SEP/GCR exposure using tumor-prone APC mouse models. Studies of acute short-term effects of modeled space radiation exposures in wildtype mouse models are more limited and necessary to better define charged particle- induced GI pathologies and test novel medical countermeasures (MCMs) to promote astronaut safety.

Methods: In this study, we performed ground-based studies where male and female C57BL/6J mice were exposed to γ-rays, 50 MeV protons, or 1 GeV/n Fe-56 ions at the NASA Space Radiation Laboratory (NSRL) with histology and immunohistochemistry endpoints measured in the first 24 h post-irradiation to define immediate SEP/GCR-induced GI alterations.

Results: Our data show that unlike matched γ-ray controls, acute exposures to protons and iron ions disrupts intestinal function and induces mucosal lesions, vascular congestion, epithelial barrier breakdown, and marked enlargement of mucosa-associated lymphoid tissue. We also measured kinetics of DNA double-strand break (DSB) repair using gamma-H2AX- specific antibodies and apoptosis via TUNEL labeling, noting the induction and disappearance of extranuclear cytoplasmic DNA marked by gamma-H2AX only in the charged particle-irradiated samples. We show that 18 h pre-treatment with curcumin-loaded nanolipoprotein particles (cNLPs) delivered via IV injection reduces DSB-associated foci levels and apoptosis and restore crypt villi lengths.

Discussion: These data improve our understanding of physiological alterations in the GI tract immediately following exposures to modeled space radiations and demonstrates effectiveness of a promising space radiation MCM.
Creating added-value filet product from rainbow trout (Oncorhynchus mykiss) by salting and smoking method: physicochemical and textural attributes
Authors: Cheng Yu-Tsung , Huang Ping-Hsiu , Lu Wen-Chien , Chu Sheng-Che , Wang Pei-Ming , Ko Wen-Chien , Li Po-Hsien
Published: May 2023

Rainbow trout (Oncorhynchus mykiss) are currently consumed as live fish, primarily for catering or consumers, as an alternative to salmon in sashimi or dishes. However, Covid-19 has hampered store and restaurant operations. Therefore, developing suitable processing conditions to extend its shelf life, such as online distribution specifications while enhancing the filets’ commercial value, would raise its production value. In this study, we investigated the fish filets salted in a 5% salt solution for 2 days and then smoked at 65°C for 4 h under different storage conditions. As result, the higher rate of salt penetration and water loss in the resolved rigor mortis group was associated with tenderization of the meat compared to the rigor mortis group. Thermal-shrinkage and thermal-induced tissue destruction of the smoked fish filets during processing which affects the appearance, flavor, chewiness and overall acceptability. Nevertheless, according to the results of a consumer-type evaluation, the product characteristics of the fish filets from the resolution of rigor mortis group were consistent with those of the rigor mortis group, except for a weaker aroma. Thus, these results explain the relationship between frozen stored fish and the quality of processed products. The economic concept of regulating and distributing scheduling production between raw materials and finished products in the food industry conveys promising findings that will contribute to developing sustainable food processing systems.
Case report: Be alert to herpes zoster after total knee arthroplasty
Authors: Wei Zhanqi , Weng Xisheng
Published: May 2023

An 88-year-old woman started complaining of severe pain at the right knee and above at three weeks after right total knee arthroplasty (TKA). The empirical treatment cannot effectively control the progress of pain. The lesion was eventually diagnosed to be due to herpes zoster (HZ). The finding of HZ was unexpected in this case, because HZ is extremely rare in patients after TKA.
Effect of hematoma volume on the 30-day mortality rate of patients with primary hypertensive brainstem hemorrhage: a retrospective cohort study
Authors: Yu Zhenxing , Zhang Xianbin , Xu Qiming , Zhang Zhipeng , Xia Yu , Li Huaquan , Yu Xiang , Deng Lei , Zhang Long
Published: May 2023

Objective

The purpose of this study is to investigate the effect of hematoma volume on the 30-Day Mortality Rate of patients with Primary Hypertensive Brainstem Hemorrhage (PHBH).

Methods

Retrospective analysis was done on the clinical information of 74 patients who underwent treatment for primary hypertensive brainstem hemorrhage at the Department of Neurosurgery of the 908th Hospital of the Joint Logistic Support Force of the Chinese People's Liberation Army between January 2018 and December 2021. Both univariate and multivariate logistic regression were used to assess clinical signs and risk factors that affect 30-day mortality.

Result(s)

In the 74 patients with primary hypertensive brainstem hemorrhage included in this investigation, 46 patients died and 28 patients survived. The mortality rate at 30 days was 62.16%. A statistically significant difference was seen (P < 0.001) in the results of the univariate analysis, which suggested that hematoma volume may be a factor affecting the prognosis of patients with hypertensive brainstem hemorrhage. Hematoma volume was further demonstrated to be a risk factor and an independent factor impacting death in patients with brainstem hemorrhage (P < 0.001) by multivariate logistic regression analysis (OR: 2.6, 95% CI: 1.7–3.9, P < 0.001 Crude Model, OR: 3.6, 95% CI: 1.7–7.7, P < 0.001 Multivariate-Adjusted Model). After adjusting for confounding variables such as age, body mass index, sex, history of diabetes mellitus, history of hypertension, admission GCS score, stereotactic aspiration, combined hydrocephalus, admission systolic and diastolic blood pressure, the hematoma volume was revealed to be an independent predictor of 30-day death in patients with brainstem hemorrhage. We discovered by smooth curve fitting that hematoma volume increased in a non-linear manner with 30-day mortality. The 30-day mortality rate did not alter significantly when the hematoma volume was less than 4 ml. When the hematoma volume was greater than 4 ml, the 30-day mortality rate increased rapidly, and when the hematoma volume was 10 ml, the 30-day mortality rate reached the maximum.

Conclusion(s)

Hematoma volume is an independent factor affecting 30-day mortality in patients with primary hypertensive brainstem hemorrhage. The severe and extensive neurological damage caused by primary hypertensive brainstem hemorrhage is highly unlikely to be fundamentally altered by a single protocol, and new avenues need to be explored scientifically and continuously.
Successful treatment of a blunt retrohepatic vena cava injury using an atriocaval shunt without sternotomy or thoracotomy: a case report
Authors: Choi Donghwan , Seo Dongmin , Choi Hang Jun , Moon Jonghwan
Published: May 2023

Retrohepatic vena cava (RHIVC) injury is often fatal and can be very difficult to manage. Total hepatic vascular isolation, a shunt, or bypass surgery is required for the surgical treatment of RHIVC injury in hemodynamically unstable patients; however, these are not easy procedures. Here, we present a case of RHIVC injury that was successfully treated by atriocaval shunt placement via a transdiaphragmatic incision without sternotomy or thoracotomy. In addition, we review the resuscitation and surgical procedures used for total hepatic vascular isolation in patients with RHIVC injury.