bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of PUFA-Rich Dietary Strategies on Ruminants’ Mammary Gland Gene Network: A Nutrigenomics Review
Authors: Kyriakaki Panagiota , Zisis Foivos , Pappas Athanasios C. , Mavrommatis Alexandros , Tsiplakou Eleni
Published: December 2022

Although the inclusion of polyunsaturated fatty acids (PUFAs) in ruminants’ diets appears to be a well-documented strategy to enrich milk with PUFAs, several gene networks that regulate milk synthesis and mammary gland homeostasis could be impaired. The objective of this literature review is to assess the effects of nutritional strategies focused on enriching milk with PUFAs on gene networks regulating mammary gland function and lipogenesis, as well as the impact of feed additives and bioactive compounds with prominent antioxidant potential on immune-oxidative transcriptional profiling, as a part of mammary gland homeostasis and health. The findings support the conclusion that PUFAs’ inclusion in ruminants’ diets more strongly downregulate the stearoyl-CoA desaturase (SCD) gene compared to other key genes involved in de novo fatty acid synthesis in the mammary gland. Additionally, it was revealed that seed oils rich in linoleic and linolenic acids have no such strong impact on networks that regulate lipogenic homeostasis compared to marine oils rich in eicosapentaenoic and docosahexaenoic acids. Furthermore, ample evidence supports that cows and sheep are more prone to the suppression of lipogenesis pathways compared to goats under the impact of dietary marine PUFAs. On the other hand, the inclusion of feed additives and bioactive compounds with prominent antioxidant potential in ruminants’ diets can strengthen mammary gland immune-oxidative status. Considering that PUFA’s high propensity to oxidation can induce a cascade of pro-oxidant incidences, the simultaneous supplementation of antioxidant compounds and especially polyphenols may alleviate any side effects caused by PUFA overload in the mammary gland. In conclusion, future studies should deeply investigate the effects of PUFAs on mammary gland gene networks in an effort to holistically understand their impact on both milk fat depression syndrome and homeostatic disturbance.
Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1
Authors: Louro Ricardo O. , Rusconi Giovanni , Fonseca Bruno M. , Paquete Catarina M.
Published: December 2022

Multiheme cytochromes play a central role in extracellular electron transfer, a process that allows microorganisms to sustain their metabolism with external electron acceptors or donors. In Shewanella oneidensis MR-1, the decaheme cytochromes OmcA and MtrC show functional specificity for interaction with soluble and insoluble redox partners. In this work, the capacity of extracellular electron transfer by mutant variants of S. oneidensis MR-1 OmcA was investigated. The results show that amino acid mutations can affect protein stability and alter the redox properties of the protein, without affecting the ability to perform extracellular electron transfer to methyl orange dye or a poised electrode. The results also show that there is a good correlation between the reduction of the dye and the current generated at the electrode for most but not all mutants. This observation opens the door for investigations of the molecular mechanisms of interaction with different electron acceptors to tailor these surface exposed cytochromes towards specific bio-based applications.
Evaluation of Endospore-Forming Bacteria for Suppression of Postharvest Decay of Apple Fruit
Authors: Poleatewich Anissa , Backman Paul , Nolen Haley
Published: December 2022

The use of microbial biocontrol agents for control of postharvest disease has been the subject of intensive research over the past three decades resulting in commercialization of several biocontrol products. The objective of this research was to test endospore-forming bacteria collected from apple leaves for suppression of bitter rot and blue mold on apple. Bacteria were collected from abandoned, low-input, organic, and conventionally managed orchards in Pennsylvania and were screened for their ability to produce endospores, hydrolyze chitin, reduce pathogen growth in vitro, and suppress postharvest disease in vivo. Several isolates reduced bitter rot lesion size on ‘Rome Beauty’ from 40–89% compared to untreated controls. Bacillus megaterium isolates, A3-6 and Ae-1, resulted in the greatest suppression of bitter rot lesion size. One isolate, A3-2, suppressed blue mold lesion size. Scanning electron microscopy of inoculated apple wounds suggests parasitism as a mode of action explains the suppression of bitter rot lesion size by isolate A3-6. Of the top seventeen isolates exhibiting biocontrol potential, 70% were collected from abandoned or unmanaged locations. This research demonstrates abandoned apple orchards can be a source of new biocontrol agents for control of postharvest diseases of apple.
Experimental Investigation of the Size Effect of Rock under Impact Load
Authors: Zhou Jun , Zhao Guangming , Meng Xiangrui , Liu Chonyan , Ma Longpei , Xu Wensong , Cheng Xiang
Published: December 2022

When measuring the compressive strength of rock, size and strain rate are the two main influencing factors. To study the rock strength size effect, rock specimens with length-to-diameter ratios of 0.5, 0.6, 0.7, 0.8, 0.9 and 1 were subjected to static loading tests using the RMT rock mechanics test system and dynamic loading with the split Hopkinson pressure bar, respectively. Based on the Weibull size-effect formula, the experimental results were compared with the improved formula obtained. The results show that rock strength is influenced by size and strain rate. Both the dynamic increase factor and rock strength are proportional to strain rate. The different failure modes of rock with size variation and strain rate variation are described according to the failure process of the specimens. The same length-to-diameter ratio specimens produced more fragments with a strain rate increase. Under the same strain rate of impact, the larger the rock specimen, the finer the broken fragments. Considering the factor of strain rate in the Weibull size-effect formula, the calculated result is accurate. The improved size-effect formula could be used to better elaborate the potential mechanisms of dynamic rock strength. In the unified theoretical formula containing static and dynamic loads, the relationship of rock strength, size and strain rate is well described.
Comparison of the Content of Extractives in the Bark of the Trunk and the Bark of the Branches of Silver Fir (Abies alba Mill.)
Authors: Vek Viljem , Šmidovnik Tjaša , Humar Miha , Poljanšek Ida , Oven Primož
Published: December 2022

The main objective of our study was to investigate the possible differences in the chemical composition of extractives from the bark of silver fir (Abies alba) with respect to the location of the bark sample on the tree, viz. differences in extract composition between stem bark and branch bark samples. Extractives in the bark samples from branches, depending on the distance of the sample from the trunk, were also analysed, and the stem bark samples were analysed with respect to their inner and outer parts. The results of the chemical analysis of extractives were supported by information about their antifungal and antioxidant effects. After felling and sampling silver fir trees, the collected bark samples were ground and freeze-dried. Extraction of bark samples was followed by a system of accelerated extraction using only water as a solvent. The extracts were analysed chemically using gravimetry, spectrophotometry and chromatography. Free-radical-scavenging activity was measured using the DPPH method, and the antifungal effect towards three moulds and three wood-decaying fungi was investigated with antifungal assay using the agar well diffusion method. It was found that the moisture content in bark samples decreased intensively just after the bark samples were peeled off the stem. Detailed chromatographic analysis showed that the bark extracts contained 14 compounds, among which phenolic acids, flavonoids and lignans were found to be the characteristic ones. The content of hydrophilic extractives in the branch bark samples decreased with increasing distance of the sample location from the tree stem. The largest amounts of phenolic extractives were measured in stem bark, followed by branch bark sampled at the point at which the branch entered the tree. Analysis of the separated parts of the bark showed that the outer layers of stem bark contained larger amounts of phenolic extractives, as well catechin and epicatechin, compared to the inner layers. Concentrated extracts of branch bark showed the largest free-radical-scavenging activity among the investigated samples, while strong antifungal effects of the bark extract were not found.
Synthesis and Characterization of Late Transition Metal Complexes of Mono-Acetate Pendant Armed Ethylene Cross-Bridged Tetraazamacrocycles with Promise as Oxidation Catalysts for Dye Bleaching
Authors: Hoang Tuyet , Mondal Somrita , Allen Michael B. , Garcia Leslie , Krause Jeanette A. , Oliver Allen G. , Prior Timothy J. , Hubin Timothy J.
Published: December 2022

Ethylene cross-bridged tetraazamacrocycles are known to produce kinetically stable transition metal complexes that can act as robust oxidation catalysts under harsh aqueous conditions. We have synthesized ligand analogs with single acetate pendant arms that act as pentadentate ligands to Mn, Fe, Co, Ni, Cu, and Zn. These complexes have been synthesized and characterized, including the structural characterization of four Co and Cu complexes. Cyclic voltammetry demonstrates that multiple oxidation states are stabilized by these rigid, bicyclic ligands. Yet, redox potentials of the metal complexes are modified compared to the “parent” ligands due to the pendant acetate arm. Similarly, gains in kinetic stability under harsh acidic conditions, compared to parent complexes without the pendant acetate arm, were demonstrated by a half-life seven times longer for the cyclam copper complex. Due to the reversible, high oxidation states available for the Mn and Fe complexes, the Mn and Fe complexes were examined as catalysts for the bleaching of three commonly used pollutant model dyes (methylene blue, methyl orange, and Rhodamine B) in water with hydrogen peroxide as oxidant. The efficient bleaching of these dyes was observed.
Locking the GFP Fluorophore to Enhance Its Emission Intensity
Authors: Ferreira Joana R. M. , Esteves Cátia I. C. , Marques Maria Manuel B. , Guieu Samuel
Published: December 2022

The Green Fluorescent Protein (GFP) and its analogues have been widely used as fluorescent biomarkers in cell biology. Yet, the chromophore responsible for the fluorescence of the GFP is not emissive when isolated in solution, outside the protein environment. The most accepted explanation is that the quenching of the fluorescence results from the rotation of the aryl–alkene bond and from the Z/E isomerization. Over the years, many efforts have been performed to block these torsional rotations, mimicking the environment inside the protein β-barrel, to restore the emission intensity. Molecule rigidification through chemical modifications or complexation, or through crystallization, is one of the strategies used. This review presents an overview of the strategies developed to achieve highly emissive GFP chromophore by hindering the torsional rotations.
Effect of Heavy Metal Stress on Phenolic Compounds Accumulation in Winter Wheat Plants
Authors: Jańczak-Pieniążek Marta , Cichoński Jan , Michalik Patrycja , Chrzanowski Grzegorz
Published: December 2022

Heavy metal stress can lead to many adverse effects that inhibit cellular processes at various levels of metabolism, causing a decrease in plant productivity. In response to environmental stressors, phenolic compounds fulfill significant molecular and biochemical functions in plants. Increasing the biosynthesis of phenolic compounds in plants subjected to heavy metal stress helps protect plants from oxidative stress. A pot experiment was carried out to determine the effect of the accumulation of copper (Cu) and lead (Pb) salts at concentrations of 200, 500, and 1000 ppm on seed germination, the activity of enzymes in the phenylalanine ammonia-lyase pathway (PAL) and tyrosine ammonia-lyase (TAL), along with the total phenol and flavonoid contents in seedlings of hybrid Triticum aestivum L. (winter wheat) cultivars. The accumulation of heavy metals, especially Cu, had a negative impact on the seed germination process. The cultivar “Hyacinth” reacted most strongly to heavy metal stress, which was confirmed by obtaining the lowest values of the germination parameters. Heavy metal stress caused an increase in the activity of PAL and TAL enzymes and an increase in the accumulation of phenolic compounds. Under the influence of Cu, the highest activity was shown in cv. “Hyvento” (especially at 200 ppm) and, due to the accumulation of Pb, in cv. “Hyacinth” (1000 ppm) and cv. “Hyking” (200 ppm). The cultivar “Hyking” had the highest content of phenolic compounds, which did not increase with the application of higher concentrations of metals. In other cultivars, the highest content of total phenols and flavonoids was usually observed at the lowest concentration (200 ppm) of the tested heavy metals, Cu and Pb.
Monkfish (Lophius litulon) Peptides Ameliorate High-Fat-Diet-Induced Nephrotoxicity by Reducing Oxidative Stress and Inflammation via Regulation of Intestinal Flora
Authors: Ren Xiangyu , Miao Bingtao , Cao Hongjie , Tian Xiaoxiao , Shen Lujia , Yang Zuisu , Yuan Falei , Ding Yaping
Published: December 2022

Background: Renal damage and intestinal flora imbalance due to lipotoxicity are particularly significant in terms of oxidative stress and inflammation, which can be alleviated with bioactive peptides. The monkfish (Lophius litulon) is rich in proteins, which can be used as a source of quality bioactive peptides. This study aimed to examine the protective effect of monkfish peptides on renal injury and their potential role in regulating gut microbiota. Methods: Monkfish meat was hydrolyzed using neutral protease and filtered, and the component with the highest elimination rate of 2,2-diphenyl-1-picrylhydrazyl was named lophius litulon peptides (LPs). Lipid nephrotoxicity was induced via high-fat diet (HFD) feeding for 8 weeks and then treated with LPs. Oxidative stress, inflammatory factors, and intestinal flora were evaluated. Results: LP (200 mg/kg) therapy reduced serum creatinine, uric acid, and blood urea nitrogen levels by 49.5%, 31.6%, and 31.6%, respectively. Renal vesicles and tubules were considerably improved with this treatment. Moreover, the activities of superoxide dismutase, glutathione peroxidase, and total antioxidant capacity increased significantly by 198.7%, 167.9%, 61.5%, and 89.4%, respectively. LPs attenuated the upregulation of HFD-induced Toll-like receptor 4 and phospho-nuclear factor-kappa B and increased the protein levels of heme oxygenase 1, nicotinamide quinone oxidoreductase 1, and nuclear factor erythroid 2-related factor 2. The dysbiosis of intestinal microbiota improved after LP treatment. Conclusions: LPs significantly improve antioxidant activity, reduce inflammatory cytokine levels, and regulate intestinal dysbiosis. Thus, LPs are potential compounds that can alleviate HFD-induced renal lipotoxicity.
Green Synthesis of Silver Nanoparticles Using Aerial Part Extract of the Anthemis pseudocotula Boiss. Plant and Their Biological Activity
Authors: Ajlouni Abdul-Wali , Hamdan Eman H. , Alshalawi Rasha Awwadh Eid , Shaik Mohammed Rafi , Khan Mujeeb , Kuniyil Mufsir , Alwarthan Abdulrahman , Ansari Mohammad Azam , Khan Merajuddin , Alkhathlan Hamad Z. , Shaik Jilani P. , Adil Syed Farooq
Published: December 2022

Green syntheses of metallic nanoparticles using plant extracts as effective sources of reductants and stabilizers have attracted decent popularity due to their non-toxicity, environmental friendliness and rapid nature. The current study demonstrates the ecofriendly, facile and inexpensive synthesis of silver nanoparticles (AP-AgNPs) using the extract of aerial parts of the Anthemis pseudocotula Boiss. plant (AP). Herein, the aerial parts extract of AP performed a twin role of a reducing as well as a stabilizing agent. The green synthesized AP-AgNPs were characterized by several techniques such as XRD, UV-Vis, FT-IR, TEM, SEM and EDX. Furthermore, the antimicrobial and antibiofilm activity of as-prepared AP-AgNPs were examined by a standard two-fold microbroth dilution method and tissue culture plate methods, respectively, against several Gram-negative and Gram-positive bacterial strains and fungal species such as Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), multidrug-resistant Pseudomonas aeruginosa (MDR-PA) and Acinetobacter baumannii (MDR-AB), methicillin-resistant S. aureus (MRSA) and Candida albicans (C. albicans) strains. The antimicrobial activity results clearly indicated that the Gram-negative bacteria MDR-PA was most affected by AgNPs as compared to other Gram-negative and Gram-positive bacteria and fungi C. albicans. Whereas, in the case of antibiofilm activity, it has been found that AgNPs at 0.039 mg/mL, inhibit biofilms formation of Gram-negative bacteria i.e., MDR-PA, E. coli, and MDR-AB by 78.98 ± 1.12, 65.77 ± 1.05 and 66.94 ± 1.35%, respectively. On the other hand, at the same dose (i.e., 0.039 mg/mL), AP-AgNPs inhibits biofilm formation of Gram-positive bacteria i.e., MRSA, S. aureus and fungi C. albicans by 67.81 ± 0.99, 54.61 ± 1.11 and 56.22 ± 1.06%, respectively. The present work indicates the efficiency of green synthesized AP-AgNPs as good antimicrobial and antibiofilm agents against selected bacterial and fungal species.
Simultaneous Measurement of Cortisol, Cortisone, Dexamethasone and Additional Exogenous Corticosteroids by Rapid and Sensitive LC–MS/MS Analysis
Authors: Ponzetto Federico , Parasiliti-Caprino Mirko , Settanni Fabio , Nonnato Antonello , Mengozzi Giulio , Ghigo Ezio , Giordano Roberta
Published: December 2022

The simultaneous measurement of dexamethasone and cortisol has proven the ability to increase the diagnostic performance of the overnight dexamethasone-suppression test. Furthermore, the therapeutic drug monitoring of administered corticosteroid drugs could represent a crucial tool for investigating unexpected variations of steroid hormones’ circulating levels. In this work, an LC–MS/MS method for the quantification of cortisol, cortisone, dexamethasone and six additional exogenous corticosteroids in the serum/plasma matrix was developed and validated in compliance with the ISO/IEC requirements. To assess the efficiency of the validated method, serum samples of 75 patients undergoing the dexamethasone-suppression test and 21 plasma samples of patients under immunosuppressive treatment after kidney transplant were analyzed. In all dexamethasone-suppression test samples, it was possible to measure the circulating levels of cortisol, cortisone and dexamethasone. Concentrations of the latter were for all tested patients above the proposed cutoff for the dexamethasone-suppression test’s results, and the cortisol concentrations showed good correlation with the ones measured by routine immunometric analysis, therefore confirming the screening outcome for all enrolled patients. Prednisone was detected and quantified in all enrolled patients, confirming the use of such a corticosteroid for immunosuppressive therapy. Thanks to these two applications, we proved the overall performance of the developed LC–MS/MS method for four target analytes. The future implementation of such an analytical tool in the clinical biochemistry laboratory’s routine will guarantee a single and versatile tool for simultaneously monitoring dexamethasone-suppression-test results and corticosteroid drugs’ administration.
Intense Blue Photo Emissive Carbon Dots Prepared through Pyrolytic Processing of Ligno-Cellulosic Wastes
Authors: Stan Loredana , Volf Irina , Stan Corneliu S. , Albu Cristina , Coroaba Adina , Ursu Laura E. , Popa Marcel
Published: December 2022

In this work, Carbon Dots with intense blue photo-luminescent emission were prepared through a pyrolytic processing of forestry ligno-cellulosic waste. The preparation path is simple and straightforward, mainly consisting of drying and fine grinding of the ligno-cellulosic waste followed by thermal exposure and dispersion in water. The prepared Carbon Dots presented characteristic excitation wavelength dependent emission peaks ranging within 438–473 nm and a remarkable 28% quantum yield achieved at 350 nm excitation wavelength. Morpho-structural investigations of the prepared Carbon Dots were performed through EDX, FT-IR, Raman, DLS, XRD, and HR-SEM while absolute PLQY, steady state, and lifetime fluorescence were used to highlight their luminescence properties. Due to the wide availability of this type of ligno-cellulosic waste, an easy processing procedure achieved photo-luminescent properties, and the prepared Carbon Dots could be an interesting approach for various applications ranging from sensors, contrast agents for biology investigations, to photonic conversion mediums in various optoelectronic devices. Additionally, their biocompatibility and waste valorization in new materials might be equally good arguments in their favor, bringing a truly “green” approach.
Broadband and Incident-Angle-Modulation Near-Infrared Polarizers Based on Optically Anisotropic SnSe
Authors: Guo Zhengfeng , Gu Honggang , Yu Yali , Wei Zhongming , Liu Shiyuan
Published: December 2022

Optical anisotropy offers an extra degree of freedom to dynamically and reversibly regulate polarizing optical components, such as polarizers, without extra energy consumption and with high modulating efficiency. In this paper, we theoretically and numerically design broadband and incident-angle-modulation near-infrared polarizers, based on the SnSe, whose optical anisotropy is quantitatively evaluated by the complete dielectric tensor, complex refractive index tensor, and derived birefringence (~|Δn|max = 0.4) and dichroism (~|Δk|max = 0.4). The bandwidth of a broadband polarizer is 324 nm, from 1262 nm to 1586 nm, with an average extinction ratio above 23 dB. For the incident-angle-modulation near-infrared polarizer, the high incident angles dynamically and reversibly modulate its working wavelength with a maximum extinction ratio of 71 dB. Numerical simulations and theoretical calculations reveal that the considerable absorption for p light and continuously and relatively low absorption of s light lead to the broadband polarizer, while the incident-angle-modulation one mainly arises from the blue shift of corresponding wavelength of p light’s minimum reflectance. The proposed novel design of polarizers based on SnSe are likely to be mass-produced and integrated into an on-chip system, which opens up a new thought to design polarizing optical components by utilizing other low-symmetry materials.
Miniaturized Spoof Plasmonic Antennas with Good Impedance Matching
Authors: Ren Yi , Zhang Jingjing , Gao Xinxin , Zheng Xin , Zhang Le Peng , Cui Tie Jun
Published: December 2022

The ability of spoof surface plasmon polaritons (SSPPs) to confine electromagnetic fields in a subwavelength regime enables the design of miniaturized antennas. However, the impedance matching scheme for miniaturized spoof plasmonic antennas has not been studied systematically. In this paper, we propose a general method in the antenna design based on SSPPs, providing a feasible solution to impedance matching at the feeding point of miniaturized spoof plasmonic antennas. To verify the method, a prototype of a planar spoof plasmonic dipole antenna is simulated, fabricated and measured, of which the dipole arm length is reduced by 35.2% as compared with the traditional dipole antenna. A peak gain level of 4.29 dBi and the radiation efficiency of about 94.5% were measured at 6 GHz. This general method can be extended to solve the impedance matching problem in the design of other spoof plasmonic devices.
Graphene Oxide Deposited with Transition Metal Chalcogenide for Selective Extraction and Determination of Hg(II): Experimental and Computational Analysis
Authors: Alsaeedi Huda , Ahmad Hilal , Altowairqi Malak Faisal , Almuryyi Nouf AbdulRahman , Alsalme Ali
Published: December 2022

A graphene oxide (GO/CdS) nanocomposite was synthesized by an in situ hydrothermal process and studied to develop a micro solid phase extraction procedure. Microscopic and spectroscopic characterizations have confirmed the successful preparation of the GO/CdS composite. The prepared nanocomposite selectively extracts Hg(II) ions from various water samples (tap, river, and groundwater). The intriguing characteristic of GO sheets is to provide exceptional hydrophilicity and Hg(II) accessibility to surface-decorated CdS nanoparticles. The GO/CdS nanocomposite shows excellent extraction of trace Hg(II) in a short interval of time. Computations based on density functional theory (DFT) suggest that energetically favorable multinuclear S-Hg binding leads to rapid adsorption with high sorption capacity at GO/CdS sites. The analytical features of merit suggested that the developed method has a low detection limit (0.07 µg L−1) and shows good accuracy and precision (with RSD 3.5%; N = 5). The developed method was verified by analyzing SRM 1641d (Standard Reference Material) and real samples after spiking to a predetermined amount.
Acoustic Plasmons in Nickel and Its Modification upon Hydrogen Uptake
Authors: Koroteev Yury M. , Silkin Igor V. , Chernov Ivan P. , Chulkov Evgueni V. , Silkin Vyacheslav M.
Published: December 2022

In this work, we study, in the framework of the ab initio linear-response time-dependent density functional theory, the low-energy collective electronic excitations with characteristic sound-like dispersion, called acoustic plasmons, in bulk ferromagnetic nickel. Since the respective spatial oscillations in slow and fast charge systems involve states with different spins, excitation of such plasmons in nickel should result in the spatial variations in the spin structure as well. We extend our study to NiHx with different hydrogen concentrations x. We vary the hydrogen concentration and trace variations in the acoustic plasmons properties. Finally, at x=1 the acoustic modes disappear in paramagnetic NiH. The explanation of such evolution is based on the changes in the population of different energy bands with hydrogen content variation.
The Ultrahigh Adsorption Capacity and Excellent Photocatalytic Degradation Activity of Mesoporous CuO with Novel Architecture
Authors: Ni Jing , Lei Jianfei , Wang Zhaowu , Huang Lanlan , Zhu Hang , Liu Hai , Hu Fuqiang , Qu Ting , Yang Huiyu , Yang Haiyang , Gong Chunli
Published: December 2022

In this paper, mesoporous CuO with a novel architecture was synthesized through a conventional hydrothermal approach followed by a facile sintering procedure. HR-TEM analysis found that mesoporous CuO with an interconnected pore structure has exposed high-energy crystal planes of (002) and (200). Theoretical calculations indicated that the high-energy crystal planes have superior adsorption capacity for H+ ions, which is critical for the excellent adsorption and remarkable photocatalytic activity of the anionic dye. The adsorption capacity of CuO to methyl orange (MO) at 0.4 g/L was approximately 30% under adsorption equilibrium conditions. We propose a state-changing mechanism to analyze the synergy and mutual restraint relation among the catalyst CuO, H+ ions, dye and H2O2. According to this mechanism, the degradation rate of MO can be elevated 3.5 times only by regulating the MO ratio in three states.
Band Gap Tuning in Transition Metal and Rare-Earth-Ion-Doped TiO2, CeO2, and SnO2 Nanoparticles
Authors: Apostolova Iliana , Apostolov Angel , Wesselinowa Julia
Published: December 2022

The energy gap Eg between the valence and conduction bands is a key characteristic of semiconductors. Semiconductors, such as TiO2, SnO2, and CeO2 have a relatively wide band gap Eg that only allows the material to absorb UV light. Using the s-d microscopic model and the Green’s function method, we have shown two possibilities to reduce the band-gap energy Eg—reducing the NP size and/or ion doping with transition metals (Co, Fe, Mn, and Cu) or rare earth (Sm, Tb, and Er) ions. Different strains appear that lead to changes in the exchange-interaction constants, and thus to a decrease in Eg. Moreover, the importance of the s-d interaction, which causes room-temperature ferromagnetism and band-gap energy tuning in dilute magnetic semiconductors, is shown. We tried to clarify some discrepancies in the experimental data.
Effect of Lycopene Intake on the Fasting Blood Glucose Level: A Systematic Review with Meta-Analysis
Authors: Inoue Takuro , Yoshida Kazutaka , Sasaki Erika , Aizawa Koichi , Kamioka Hiroharu
Published: December 2022

Lycopene is a lipophilic unsaturated carotenoid exhibiting a strong singlet oxygen-quenching ability. Herein, we investigated the effect of lycopene intake on the fasting blood glucose (FBG) level by conducting a systematic review and meta-analyses. We searched 15 databases (from the earliest date to June 2022 for PubMed or to August or September 2018 for the other databases) and included human interventional studies that assessed the effects of oral lycopene intake on FBG levels of participants ≥ 18 years of age. Three authors independently selected applicable studies and then assessed the study quality. Data were pooled as standardized mean difference (SMD) and analyzed by the random-effects model. Heterogeneity was assessed by I2 statistics. A meta-analysis including 11 trial arms (n = 750) revealed a tendency towards a significant decrease in FBG level with not-important heterogeneity [SMD = −0.15 (95% CI: −0.31, 0.00), p = 0.05, I2 = 9%]. Subgroup meta-analysis including two studies (n = 152) in type 2 diabetes patients revealed significantly decreased FBG levels with not-important heterogeneity [SMD = −0.37 (95% CI: −0.69, −0.05), p = 0.02, I2 = 0%]. Most studies meeting the eligibility criteria had a moderate risk of bias. The funnel plot for FBG suggested an absence of publication bias. In conclusion, this systematic review and meta-analyses suggested that lycopene intake exerted an FBG-decreasing effect.
The Benefits to Bone Health in Children and Pre-School Children with Additional Exercise Interventions: A Systematic Review and Meta-Analysis
Authors: McCaskie Callum , Siafarikas Aris , Cochrane Wilkie Jodie , Sutton Vanessa , Chivers Paola , Hart Nicolas H. , Murphy Myles C.
Published: December 2022

Objective: Determine if exercise interventions, beyond what is already provided to children and preschool children, improve bone health and reduce fracture incidence. Design: Systematic review and meta-analysis reported using the PRISMA guidelines. Certainty of evidence was assessed using GRADE recommendations. Data sources: Five electronic databases were searched for records: PUBMED; CINAHL; CENTRAL; SPORTDiscus; Web of Science. Eligibility criteria for selecting studies: Randomised, quasi-randomised and non-randomised controlled trials (including cluster-randomised) assessing the impact of additional exercise interventions (e.g., increased physical education classes or specific jumping programs) on bone health in children (6–12 years) and pre-school children (2–5 years) without dietary intervention. Results: Thirty-one records representing 16 distinct clinical trials were included. Dual-energy X-ray Absorptiometry (DXA) and/or peripheral Quantitative Computed Tomography (pQCT) were used to quantify bone health. Increased femoral neck bone mineral content in children with additional exercise interventions (n = 790, SMD = 0.55, 95% CI = 0.01 to 1.09) was reported, however this was not significant following sensitivity analysis. Other DXA and pQCT measures, as well as fracture incidence, did not appear to significantly differ over time between intervention and control groups. No studies reported adverse events. Studies failed to report all domains within the TIDieR checklist. All studies were at high risk of bias using the Cochrane RoB Tool 2.0. The certainty of the evidence was very low. Conclusions: The addition of exercise interventions, beyond what is provided to children, does not appear to improve DXA and pQCT measures of bone health. The effect of additional exercise interventions on bone health in pre-school children is largely unknown. Future trials should ensure adherence is clearly reported and controlled for within analysis as well as including reports of adverse events (e.g., apophysitis) that occur due to increased exercise interventions.
Determinants of Changes in the Diet Quality of Japanese Adults during the Coronavirus Disease 2019 Pandemic
Authors: Hayashi Fumi , Takemi Yukari
Published: December 2022

This study evaluated changes in diet quality during the coronavirus disease 2019 (COVID-19) pandemic and its association with variations in attitudes or behavior, as well as health status changes. Participants were Japanese adults aged 20–69 years who resided in 13 prefectures where specific cautions were announced to prevent the spread of the virus. An online survey was conducted in September 2021, and participants were those who shopped for food or prepared meals more than twice a week during the survey. Overall, 2101 participants were analyzed. An improved or worsened diet quality was determined based on changes in food consumption patterns, and participants were categorized into three groups (improved diet quality (IDQ), worsened diet quality (WDQ), and others). The IDQ group participants (10.2%) improved their dietary consciousness during COVID-19, cooked almost everything from ingredients, and increased their balanced meal eating frequency. However, the WDQ participants (11.1%) had worsened dietary consciousness and increased consumption of takeaway meals and alcohol but decreased balanced meal consumption. Cooking frequency changes were not independent determinants of variations in diet quality. Our results show that the diet quality changes during COVID-19 were possibly caused by changes in dietary consciousness or different levels of meal preparation practices.
Vitamin D, Folic Acid and Vitamin B12 Can Reverse Vitamin D Deficiency-Induced Learning and Memory Impairment by Altering 27-Hydroxycholesterol and S-Adenosylmethionine
Authors: Wang Lijing , Zhou Cui , Yu Huiyan , Hao Ling , Ju Mengwei , Feng Wenjing , Guo Zhiting , Sun Xuejing , Fan Qiushi , Xiao Rong
Published: December 2022

The cholesterol-oxidized metabolite 27-hydroxycholesterol (27-OHC) is synthesized by CYP27A1, which is a key factor in vitamin D and oxysterol metabolism. Both vitamin D and 27-OHC are considered to play important roles in Alzheimer’s disease (AD). The study aims to research the effects of co-supplementation of vitamin D, folic acid, and vitamin B12 on learning and memory ability in vitamin D-deficient mice, and to explore the underlying mechanism. In this study, C57BL/6J mice were fed a vitamin D-deficient diet for 13 weeks to establish a vitamin D-deficient mice model. The vitamin D-deficient mice were then orally gavaged with vitamin D (VD), folic acid (FA), and vitamin B12 (VB12) alone or together for eight weeks. Following the gavage, the learning and memory ability of the mice were evaluated by Morris Water Maze and Novel object recognition test. The CYP27A1-related gene and protein expressions in the liver and brain were determined by qRT-PCR. The serum level of 27-OHC was detected by HPLC-MS. Serum levels of 25(OH)D, homocysteine (Hcy), and S-Adenosylmethionine (SAM) were measured by ELISA. After feeding with the vitamin D-deficient diet, the mice performed longer latency to a platform (p < 0.001), lower average speed (p = 0.026) in the Morris Water Maze, a lower time discrimination index (p = 0.009) in Novel object recognition, and performances were reversed after vitamin D, folic acid and vitamin B12 supplementation alone or together (p < 0.05). The gene expressions of CYP27A1 in the liver and brain were upregulated in the vitamin D-deficiency (VDD) group compared with the control (CON) group (p = 0.015), while it was downregulated in VDD + VD and VDD + VD-FA/VB12 groups compared with the VDD group (p < 0.05), with a similar trend in the protein expression of CYP27A1. The serum levels of 27-OHC were higher in the VDD group, compared with CON, VDD + VD, and VDD + VD-FA/VB12 group (p < 0.05), and a similar trend was found in the brain. The serum 25(OH)D levels were significantly decreased in the vitamin D-deficiency group (p = 0.008), and increased in the vitamin D-supplemented group (p < 0.001). The serum levels of SAM were higher in the B vitamins-supplemented group, compared with CON and VDD groups (p < 0.05). This study suggests that CYP27A1 expression may be involved in the mechanism of learning and memory impairment induced by vitamin D deficiency. Co-supplementation with vitamin D, folic acid, and vitamin B12 significantly reverses this effect by affecting the expression of CYP27A1, which in turn regulates the metabolism of 27-OHC, 25(OH)D, and SAM.
Predictors of Effectiveness and Adherence in a Multimodal Obesity Treatment Program for Children and Adolescents in Routine Care
Authors: Breinker Julius Lars , Kaspar Anika , Sergeyev Elena , Körner Antje , Kiess Wieland , Hilbert Anja
Published: December 2022

Multimodal obesity treatments for children and adolescents generally showed only small to modest treatment effects and high dropout rates. Potential variations by patients’ clinical and sociodemographic factors remain, however, largely unclear. For this reason, our study analyzed psychological, physical, and sociodemographic predictors of treatment success and adherence in a multimodal obesity treatment over 12 months. The intent-to-treat sample included n = 361 children and adolescents (ages 3–17 years), of which n = 214 or 59.28% of patients completed treatment. A younger age and, in the sensitivity analysis, additionally a greater eating disorder psychopathology and treatment initiation before COVID-19 pandemic predicted greater BMI-SDS reductions (Body Mass Index-Standard Deviation Score). In contrast, predictors of treatment adherence were not found. The results underline the importance of early treatment of juvenile obesity. Additionally, eating disorder psychopathology includes restrained eating, which implies the ability to self-regulate eating behavior and therefore may have a positive effect on the treatment goal of controlled food intake. Challenges from altered treatment procedures due to the COVID-19 pandemic nonetheless remain.
Quantitative Diet, Body Composition and Sprint Performance in Female Professional Beach Handball Players
Authors: Martínez-Rodríguez Alejandro , Sánchez-Sánchez Javier , Martínez-Olcina María , Vicente-Martínez Manuel , Peñaranda-Moraga Marcelo , Asencio-Mas Nuria , Gonzálvez-Alvarado Lucía , Matlosz Piotr , Yáñez-Sepúlveda Rodrigo , Cortés-Roco Guillermo , Sánchez-Sáez Juan Antonio
Published: December 2022

Women’s elite sports have experienced an exponential increase in the last decade, as has beach handball (BH). The high demands of this sport mean that athletes need to be in superior physical condition, so nutrition and body composition are determining factors in their sporting performance. For this reason, the aim of this study was to analyze, compare and correlate the most relevant variables of food intake (quantitative), body composition (focus on the bone mass characteristics) and sprint performance in female professional BH players. Thirty-three women from the National Spanish Team participated in this study. Dietary assessment, anthropometric measurements and sprint tests were performed. In general, the players had a low carbohydrate intake and adequate protein intake, with no significant differences depending on the category and playing position. For senior players, positive correlations were found between protein intake and bone mass (r = 0.584, p = 0.022), polyunsaturated fatty acid intake and muscle mass (r = 0.387, p = 0.026) and finally between fat mass and animal protein intake (r = 0.569, p = 0.027). Body composition was similar in both categories; however, goalkeepers had the highest fat (22.6 ± 3.86%, 16.2 ± 4.84 kg) component (vs. wings: 17.4 ± 3.53%, p = 0.031/vs. specialists: 11.1 ± 1.91 kg, p = 0.034), and senior players had higher muscle mass (kilograms). It is worth noting the finding that players with a greater trochanter height had significantly lower sprint times (p = 0.014 and p = 0.048 for 5 and 10 m, respectively). Certain bone characteristics, such as iliospinale height, biacromial and bimalleolar diameters, mesosternal perimeter and biceps skinfold, differ depending on the position. In addition, the greater speed of the senior players may be due to the greater specialization, number of training sessions performed and specific bone characteristics, such as trochanter height. In this regard, the data provided in this study will assist with establishing criteria for the selection of talent for this sporting discipline.
Lactobacillus gasseri JM1 Isolated from Infant Feces Alleviates Colitis in Mice via Protecting the Intestinal Barrier
Authors: Cheng Shasha , Li Hongxuan , Huang Yan , Su Yue , Li Yu , Jia Ai , Jiang Yujun , Zhang Yu , Man Chaoxin
Published: December 2022

Ulcerative colitis (UC) is a chronic and recurrent inflammatory bowel disease, and the intestinal barrier is an important line of defense against intestinal disease. Herein, we investigated the effect of Lactobacillus gasseri JM1 at different doses (1 × 106, 1 × 107, 1 × 108 CFU/day) on colitis mice and explored the possible mechanism. The results showed that L. gasseri JM1 alleviated DSS-induced colitis in mice, with reductions in disease activity index (DAI), histological scores and myeloperoxidase activity as well as alleviation of colonic shortening. Furthermore, L. gasseri JM1 regulated the levels of inflammatory cytokines TNF-α, IL-6, IL-1β, and IL-10; restored the expression of Claudin-3, Occludin, ZO-1, and MUC2; and increased the number of goblet cells and acidic mucin. The 16S rDNA sequencing results indicated that intervention with L. gasseri JM1 balanced the gut microbiota structure by elevating the abundance of beneficial bacteria (Oscillospira, Clostridium and Ruminococcus) and decreasing that of harmful bacteria (Shigella and Turicibacter). Meanwhile, the contents of short-chain fatty acids (SCFAs) increased. In conclusion, L. gasseri JM1 could alleviate intestinal barrier damage in colitis mice by modulating the tight junction structures, intestinal mucus layer, inflammatory cytokines, gut microbiota, and SCFAs. It can be considered a potential preventive strategy to alleviate colitis injury.
African American Women with Cardiometabolic Complications of Pregnancy Have Decreased Serum Abundance of Specialized Pro-Resolving Lipid Mediators and Endocannabinoids
Authors: Maner-Smith Kristal M. , Ferranti Erin , Dunlop Anne , Corwin Elizabeth , Ortlund Eric A.
Published: December 2022

African American (AA) women experience higher rates of maternal morbidity and mortality compared to US women of other racial/ ethnic groups. Cardiometabolic complications of pregnancy (including gestational diabetes, gestational hypertension, and preeclampsia) are leading contributors to maternal morbidity and mortality. Marked changes in circulating lipids are known to accompany cardiometabolic complications of pregnancy. Serum concentrations of docosahexaenoic acid (DHA) have been shown to be inversely correlated with risk for preeclampsia. DHA is a biosynthetic precursor of a class of specialized pro-resolving mediators (SPMs), resolvins, that have anti-inflammatory properties and are also associated with hypertensive disorders of pregnancy. We employed targeted lipidomics to characterize the distribution of DHA-containing phospholipids and SPMs in maternal serum collected in early and late pregnancy (8–14 weeks and 24–30 weeks gestation, respectively) to identify key lipids that are dysregulated during pregnancy in AA women who develop cardiometabolic complications. We identified a lipid signature in early pregnancy serum samples of AA women that is predictive of cardiometabolic complications of pregnancy with 74% accuracy. These are Resolvin D1, Resolvin E1, 2-AG, PGE2-glyerol ester, and 36:6 PC. These findings suggest that there are blood-based markers detectable in early pregnancy that can potentially identify persons at risk and tailor clinical interventions.
Diet Quality and Nutrition Behavior of Federal Nutrition Education Program Participants before and during the COVID-19 Pandemic
Authors: Sankavaram Kavitha , Roe Annie J. , Whiteley Jolene , Price William J.
Published: December 2022

Despite challenges due to the COVID-19 pandemic, reports from regional and national meetings of the Expanded Food and Nutrition Education program (EFNEP) have provided anecdotal evidence that the program has persevered, pivoted, and continued to positively impact the lives of some of the nation’s most vulnerable populations. However, there have been necessary changes to program delivery, inevitable changes in the lives of participants, and changes in the food environment that may have impacted program outcomes. This study compares national EFNEP data (demographics, behavior change data, and 24 h dietary recall data) of participants from two federal fiscal years, before the COVID-19 pandemic and during the pandemic. Linear mixed model analysis of variance and covariance were used to assess the effects of year on program outcomes. Results of this study provide quantitative evidence of the resiliency of EFNEP to facilitate positive behavior changes related to diet quality, physical activity, food safety, food resource management, and food security. Amidst changes in the food environment during the COVID-19 pandemic, these results emphasize the importance and value of federal nutrition education programs in any food environment.
Nicotinamide Mononucleotide Ameliorates Silica-Induced Lung Injury through the Nrf2-Regulated Glutathione Metabolism Pathway in Mice
Authors: Wang Liqun , Zhao Manyu , Qian Rui , Wang Mengzhu , Bao Qixue , Chen Xuxi , Du Wen , Zhang Ling , Ye Tinghong , Xie Yongmei , Zhang Ben , Peng Lijun , Yao Yuqin
Published: December 2022

Nicotinamide mononucleotide (NMN) is a natural antioxidant approved as a nutritional supplement and food ingredient, but its protective role in silicosis characterized by oxidative damage remains unknown. In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis. We found that NMN mitigated lung damage at 7 and 28 days, manifested as a decreasing coefficient of lung weight and histological changes, and alleviated oxidative damage by reducing levels of reactive oxygen species and increasing glutathione. Meanwhile, NMN treatment also reduced the recruitment of inflammatory cells and inflammatory infiltration in lung tissue. Transcriptomic analysis showed that NMN treatment mainly regulated immune response and glutathione metabolism pathways. Additionally, NMN upregulated the expression of antioxidant genes Gstm1, Gstm2, and Mgst1 by promoting the expression and nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2). Gene interaction analysis showed that Nrf2 interacted with Gstm1 and Mgst1 through Gtsm2. Promisingly, oxidative damage mediated by these genes occurred mainly in fibroblasts. In summary, NMN alleviates silica-induced oxidative stress and lung injury by regulating the endogenous glutathione metabolism pathways. This study reveals that NMN supplementation might be a promising strategy for mitigating oxidative stress and inflammation in silicosis.