bluuebunny/crossref_metadata_2025 huggingface dataset:
Perceived Feasibility of Endoscopic Ultrasound-Guided Gastroenteric Anastomosis: An Italian Survey
Authors: Tarantino Ilaria , Sinagra Emanuele , Binda Cecilia , Fugazza Alessandro , Amato Arnaldo , Maida Marcello , Lisotti Andrea , Crinò Stefano Francesco , Aragona Giovanni , Fabbri Carlo , Anderloni Andrea ,   on behalf of the i-EUS Group
Published: April 2022

Background and Objectives: Endoscopic ultrasound-guided gastroenteric anastomosis (EUS-GEA) using lumen-apposing metal stents (LAMS) is emerging as a minimally invasive alternative to surgery across several indications. The aim of this survey is to investigate the perceived feasibility of this technique nationwide, within a working group skilled in interventional endosonography. Materials and Methods: Endoscopists were asked to answer to 49 items on a web-based questionnaire about expertise, peri- and intra-procedural aspects in the three main settings of EUS-GEA performance, budget/refund, and future perspectives. Statistical analysis was performed through SPSS® (IBM Corp. Released 2017. IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY: IBM Corp.). Results: Sixty endosonographers belonging to forty Italian centers were I-EUS app users and were all invited to participate. In total, 29 participants from 24 Italian centers completed the survey. All the participants were endosonographers with a broad range of experience both in the field of EUS (only 10.3% with more than 20 years of experience) and duodenal stenting (only 6.9% placed more than 10 stents in 2020), whereas 86.2% also performed ERCP. A total of 27.6% of participants performed EUS-GEA (3.4% more than 20 during their career); on the other hand, 79.3% of participants routinely performed drainage of peri-pancreatic fluid collections, 62.1% performed biliary drainage, and 62.1% performed gallbladder drainage with LAMS. A total of 89.7% of participants thought that EUS-GEA could be useful in their daily clinical practice, with 100% concluding that this procedure will need to be performed in referral centers in the near future; however, in 55.2% of cases, organizational obstacles may occur and affect the diffusion of the procedure. With regard to indications: 44.8% of participants performed the procedure with palliative intent for malignant indication (96.6% pancreatic adenocarcinoma), and 13.6% also for benign indication. A total of 20.7% of participants experienced adverse events (none severe or fatal, 66.6% moderate). A total of 62.1% of participants considered the procedure technically challenging, although 82.8% considered the risk of adverse events acceptable when considering the benefit. Conclusions: To our knowledge, this is the first survey assessing the perceived feasibility of EUS-guided anastomoses after its advent. There are currently wide variations in practice nationwide, which demonstrate a need to define technical, qualitative, and peri-procedural requirements to carry out this procedure. Therefore, a standardization of these requirements is needed in order to overcome the technical, economical, and organizational obstacles relative to its diffusion.
Correction: Vabalaite et al. Effects of High-Frequency (HF) Repetitive Transcranial Magnetic Stimulation (rTMS) on Upper Extremity Motor Function in Stroke Patients: A Systematic Review. Medicina 2021, 57, 1215
Authors: Vabalaite Birute , Petruseviciene Laura , Savickas Raimondas , Kubilius Raimondas , Ignatavicius Povilas , Lendraitiene Egle
Published: April 2022

In the original publication [...]
Long-Term Survival Impact of High-Grade Complications after Liver Resection for Hepatocellular Carcinoma: A Retrospective Single-Centre Cohort Study
Authors: Kuo Chin-Wen , Wu Hsiang-Ling , Li Chun-Cheng , Cata Juan P. , Liu Hsin-Yi , Hou Ming-Chih , Cherng Yih-Giun , Tai Ying-Hsuan
Published: April 2022

Background and Objectives: Although complications after liver resection for hepatic cancer are common, the long-term impact of these complications on oncological outcomes remains unclear. This study aimed to investigate the potential effect of high-grade postoperative complications on long-term mortality and cancer recurrence after surgical resection of hepatocellular carcinoma. Materials and Methods: In a retrospective cohort study, patients undergoing curative liver resection for primary hepatocellular carcinoma between 2005 and 2016 were evaluated. The Clavien–Dindo (CD) grading system was used to classify patients into two groups of either high-grade complications (grade III or IV) or none or low-grade complications (grade 0 to II) within 30 days after surgery. The primary endpoint was all-cause mortality. Secondary endpoints were cancer-specific mortality and cancer recurrence. Weighted Cox proportional hazards regression models were used to calculate the adjusted hazard ratio (aHR) with a 95% confidence interval (CI) for the outcomes of interest. Results: A total of 1419 patients with a median follow-up time of 46.6 months were analysed. Among them, 93 (6.6%) developed high-grade complications after surgery. The most common complications were bile leakage (n = 30) in CD grade III and respiratory failure (n = 13) in CD grade IV. High-grade complications were significantly associated with all-cause mortality (aHR: 1.78, 95% CI: 1.55–2.06) and cancer-specific mortality (aHR: 1.34, 95% CI: 1.13–1.60), but not cancer recurrence (aHR: 0.92, 95% CI: 0.84–1.02). Independent influential factors for complications were sex, diabetes mellitus, clinically significant portal hypertension, oesophageal varices, multifocal cancer, intraoperative blood loss, and anaesthesia duration. Conclusions: Patients who had high-grade postoperative complications had a greater risk of long-term mortality after liver resection for hepatocellular carcinoma. Prevention of postoperative complications may serve as an effective strategy for improving long-term survival.
Design and Modeling of MEMS Microgrippers for Laser-Based Additive Manufacturing
Authors: De Pasquale Giorgio
Published: April 2022

The geometrical constraints and dimensional tolerances lead to specific design issues of MEMS manipulators for biological applications. The target properties become even more important in the case of in vitro manipulation of cells. Several design solutions have been proposed in the literature, however, some issues related to the thermal heating of microgripper tips and to the electric voltage effects still remain unsolved. This paper reports the design for additive manufacturing (DFAM) of micro-electro mechanical systems (MEMS) microgrippers. The design limitations imposed by the micro-stereolithography fabrication process are considered. The design solution proposed in this study is based on compliant structures and external actuation; this layout provides the potential elimination of the main issues related to cells micro-manipulators represented by the excessive thermal heating and the voltage exposure of samples. The simulation through finite elements method (FEM) models of the structure in terms of force–displacement relation and stress distribution supports the design evolution proposed.
A New Early Predictor of Fatal Outcome for COVID-19 in an Italian Emergency Department: The Modified Quick-SOFA
Authors: Guarino Matteo , Perna Benedetta , Remelli Francesca , Cuoghi Francesca , Cesaro Alice Eleonora , Spampinato Michele Domenico , Maritati Martina , Contini Carlo , De Giorgio Roberto
Published: April 2022

Background: Since 2019, the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing a rapidly spreading pandemic. The present study aims to compare a modified quick SOFA (MqSOFA) score with the NEWS-2 score to predict in-hospital mortality (IHM), 30-days mortality and recovery setting. Methods: All patients admitted from March to October 2020 to the Emergency Department of St. Anna Hospital, Ferrara, Italy with clinically suspected SARS-CoV-2 infection were retrospectively included in this single-centre study and evaluated with the MqSOFA and NEWS-2 scores. Statistical and logistic regression analyses were applied to our database. Results: A total of 3359 individual records were retrieved. Among them, 2716 patients were excluded because of a negative nasopharyngeal swab and 206 for lacking data; thus, 437 patients were eligible. The data showed that the MqSOFA and NEWS-2 scores equally predicted IHM (p < 0.001) and 30-days mortality (p < 0.001). Higher incidences of coronary artery disease, congestive heart failure, cerebrovascular accidents, dementia, chronic kidney disease and cancer were found in the deceased vs. survived group. Conclusions: In this study we confirmed that the MqSOFA score was non-inferior to the NEWS-2 score in predicting IHM and 30-days mortality. Furthermore, the MqSOFA score was easier to use than NEWS-2 and is more suitable for emergency settings. Neither the NEWS-2 nor the MqSOFA scores were able to predict the recovery setting.
Methods, Models and Tools for Improving the Quality of Textual Annotations
Authors: Artese Maria Teresa , Gagliardi Isabella
Published: April 2022

In multilingual textual archives, the availability of textual annotation, that is keywords either manually or automatically associated with texts, is something worth exploiting to improve user experience and successful navigation, search and visualization. It is therefore necessary to study and develop tools for this exploitation. The paper aims to define models and tools for handling textual annotations, in our case keywords of a scientific library. With the background of NLP, machine learning and deep learning approaches are presented. They allow us, in supervised and unsupervised ways, to increase the quality of keywords. The different steps of the pipeline are addressed, and different solutions are analyzed, implemented, evaluated and compared, using statistical methods, machine learning and artificial neural networks as appropriate. If possible, off-the-shelf solutions will also be compared. The models are trained on different datasets already available or created ad hoc with common characteristics with the starting dataset. The results obtained are presented, commented and compared with each other.
Experimental and Computational Structural Studies of 2,3,5-Trisubstituted and 1,2,3,5-Tetrasubstituted Indoles as Non-Competitive Antagonists of GluK1/GluK2 Receptors
Authors: Bartyzel Agata , Kaczor Agnieszka A. , Mahmoudi Ghodrat , Masoudiasl Ardavan , Wróbel Tomasz M. , Pitucha Monika , Matosiuk Dariusz
Published: April 2022

The blockade of kainate receptors, in particular with non-competitive antagonists, has—due to their anticonvulsant and neuroprotective properties—therapeutic potential in many central nervous system (CNS) diseases. Deciphering the structural properties of kainate receptor ligands is crucial to designing medicinal compounds that better fit the receptor binding pockets. In light of that fact, here, we report experimental and computational structural studies of four indole derivatives that are non-competitive antagonists of GluK1/GluK2 receptors. We used X-ray studies and Hirshfeld surface analysis to determine the structure of the compounds in the solid state and quantum chemical calculations to compute HOMO and LUMO orbitals and the electrostatic potential. Moreover, non-covalent interaction maps were also calculated. It is worth emphasizing that compounds 3 and 4 are achiral molecules crystallising in non-centrosymmetric space groups, which is a relatively rare phenomenon.
In Vitro Cytotoxic Activity and Phytochemical Characterization (UPLC/T-TOF-MS/MS) of the Watermelon (Citrullus lanatus) Rind Extract
Authors: El Gizawy Heba A. , El-Haddad Alaadin E. , Attia Yasmin M. , Fahim Sally A. , Zafer Mai M. , Saadeldeen Amr M.
Published: April 2022

Reusing food waste is becoming popular in pharmaceutical industries. Watermelon (Citrullus lanatus) rind is commonly discarded as a major solid waste. Here, the in vitro cytotoxic potential of watermelon rind extracts was screened against a panel of human cancer cell lines. Cell cycle analysis was used to determine the induction of cell death, whereas annexin V-FITC binding, caspase-3, BAX, and BCL-2 mRNA expression levels were used to determine the degree of apoptosis. VEGF-promoting angiogenesis and cell migration were also evaluated. Moreover, the identification of phytoconstituents in the rind extract was achieved using UPLC/T-TOF-MS/MS, and a total of 45 bioactive compounds were detected, including phenolic acids, flavonoids aglycones, and their glycoside derivatives. The tested watermelon rind extracts suppressed cell proliferation in seven cancer cell lines in a concentration-dependent manner. The cytotoxicity of the rind aqueous extract (RAE) was higher compared with that of the other extracts. In addition to a substantial inhibitory effect on cell migration, the RAE triggered apoptosis in HCT116 and Hep2 cells by driving the accumulation of cells in the S phase and elevating the activity of caspase-3 and the BAX/BCL-2 ratio. Thus, a complete phytochemical and cytotoxic investigation of the Citrullus lanatus rind extract may identify its potential potency as an anticancer agent.
Current Knowledge on Mammalian Phospholipase A1, Brief History, Structures, Biochemical and Pathophysiological Roles
Authors: Yaginuma Shun , Kawana Hiroki , Aoki Junken
Published: April 2022

Phospholipase A1 (PLA1) is an enzyme that cleaves an ester bond at the sn-1 position of glycerophospholipids, producing a free fatty acid and a lysophospholipid. PLA1 activities have been detected both extracellularly and intracellularly, which are well conserved in higher eukaryotes, including fish and mammals. All extracellular PLA1s belong to the lipase family. In addition to PLA1 activity, most mammalian extracellular PLA1s exhibit lipase activity to hydrolyze triacylglycerol, cleaving the fatty acid and contributing to its absorption into the intestinal tract and tissues. Some extracellular PLA1s exhibit PLA1 activities specific to phosphatidic acid (PA) or phosphatidylserine (PS) and serve to produce lysophospholipid mediators such as lysophosphatidic acid (LPA) and lysophosphatidylserine (LysoPS). A high level of PLA1 activity has been detected in the cytosol fractions, where PA-PLA1/DDHD1/iPLA1 was responsible for the activity. Many homologs of PA-PLA1 and PLA2 have been shown to exhibit PLA1 activity. Although much has been learned about the pathophysiological roles of PLA1 molecules through studies of knockout mice and human genetic diseases, many questions regarding their biochemical properties, including their genuine in vivo substrate, remain elusive.
Antimicrobial Activity of Quercetin: An Approach to Its Mechanistic Principle
Authors: Nguyen Thi Lan Anh , Bhattacharya Debanjana
Published: April 2022

Quercetin, an essential plant flavonoid, possesses a variety of pharmacological activities. Extensive literature investigates its antimicrobial activity and possible mechanism of action. Quercetin has been shown to inhibit the growth of different Gram-positive and Gram-negative bacteria as well as fungi and viruses. The mechanism of its antimicrobial action includes cell membrane damage, change of membrane permeability, inhibition of synthesis of nucleic acids and proteins, reduction of expression of virulence factors, mitochondrial dysfunction, and preventing biofilm formation. Quercetin has also been shown to inhibit the growth of various drug-resistant microorganisms, thereby suggesting its use as a potent antimicrobial agent against drug-resistant strains. Furthermore, certain structural modifications of quercetin have sometimes been shown to enhance its antimicrobial activity compared to that of the parent molecule. In this review, we have summarized the antimicrobial activity of quercetin with a special focus on its mechanistic principle. Therefore, this review will provide further insights into the scientific understanding of quercetin’s mechanism of action, and the implications for its use as a clinically relevant antimicrobial agent.
Emotion Classification from Speech and Text in Videos Using a Multimodal Approach
Authors: Caschera Maria Chiara , Grifoni Patrizia , Ferri Fernando
Published: April 2022

Emotion classification is a research area in which there has been very intensive literature production concerning natural language processing, multimedia data, semantic knowledge discovery, social network mining, and text and multimedia data mining. This paper addresses the issue of emotion classification and proposes a method for classifying the emotions expressed in multimodal data extracted from videos. The proposed method models multimodal data as a sequence of features extracted from facial expressions, speech, gestures, and text, using a linguistic approach. Each sequence of multimodal data is correctly associated with the emotion by a method that models each emotion using a hidden Markov model. The trained model is evaluated on samples of multimodal sentences associated with seven basic emotions. The experimental results demonstrate a good classification rate for emotions.
Adsorption Free Energy of Cellulose Nanocrystal on Water–Oil Interface
Authors: Ito Kenya , Matsumoto Mitsuhiro
Published: April 2022

To investigate the amphiphilicity of cellulose, a series of molecular dynamics simulations were performed with a cellulose nanocrystal and a water–octane interfacial system. Assuming that the axis of cellulose is parallel to the water–octane interface, the freedoms of motion of the nanocrystal were restricted to two, the distance from the interface and the orientation around the axis. The mean force and the mean torque on the nanocrystal were evaluated with sufficiently long simulation at each crystal configuration, and their numerical integration gave a smooth free energy surface as the potential of mean force. The cellulose sample used here was found to be much more hydrophilic than oleophilic with the free energy difference ΔFw→o=318 kcal/mol. Three adsorption states with local minimum of adsorption free energy are distinguished in the free energy surface—the direct contact type which is similar to previously reported one, the hydrophilic-surface/water/octane type where a thin water layer is sandwiched between the surface and the octane phase, and the oleophilic/water/octane type where a thin water layer also exists. Water molecules in these water layers contribute to stabilize the adsorption states by taking a special orientational order and slow self-diffusion.
Efficiency and Management of Nitrogen Fertilization in Sugar Beet as Spring Crop: A Review
Authors: Varga Ivana , Jović Jurica , Rastija Mirta , Markulj Kulundžić Antonela , Zebec Vladimir , Lončarić Zdenko , Iljkić Dario , Antunović Manda
Published: April 2022

Sugar beet fertilization is a very complex agrotechnical measure for farmers. The main reason is that technological quality is equally important as sugar beet yield, but the increment of the root yield does not follow the root quality. Technological quality implies the concentration of sucrose in the root and the possibility of its extraction in the production of white table sugar. The great variability of agroecological factors that directly affect root yield and quality are possible good agrotechnics, primarily by minimizing fertilization. It should be considered that for sugar beet, the status of a single plant available nutrient in the soil is more important than the total amounts of nutrients in the soil. Soil analysis will show us the amount of free nutrients, the degree of soil acidity and the status of individual elements in the soil so that farmers can make a compensation plan. An estimate of the mineralizing ability of the soil, the N min, is very important in determining the amount of mineral nitrogen that the plant can absorb for high root yield and good technological quality. The amount of N needed by the sugar beet crop to be grown is an important factor, and it will always will be in the focus for the producers, especially from the aspect of trying to reduce the N input in agricultural production to preserve soils and their biodiversity but also to establish high yields and quality.
Psychological Interventions and Bariatric Surgery among People with Clinically Severe Obesity—A Systematic Review with Bayesian Meta-Analysis
Authors: Storman Dawid , Świerz Mateusz Jan , Storman Monika , Jasińska Katarzyna Weronika , Jemioło Paweł , Bała Małgorzata Maria
Published: April 2022

Aim: To assess the effectiveness of perioperative psychological interventions provided to patients with clinically severe obesity undergoing bariatric surgery regarding weight loss, BMI, quality of life, and psychosocial health using the Bayesian approach. Methods: We considered randomised trials that assessed the beneficial and harmful effects of perioperative psychological interventions in people with clinically severe obesity undergoing bariatric surgery. We searched four data sources from inception to 3 October 2021. The authors independently selected studies for inclusion, extracted data, and assessed the risk of bias. We conducted a meta-analysis using a Bayesian approach. PROSPERO: CRD42017077724. Results: Of 13,355 identified records, we included nine studies (published in 27 papers with 1060 participants (365 males; 693 females, 2 people with missing data)). Perioperative psychological interventions may provide little or no benefit for BMI (the last reported follow-up: MD [95% credible intervals] = −0.58 [−1.32, 0.15]; BF01 = 0.65; 7 studies; very low certainty of evidence) and weight loss (the last reported follow-up: MD = −0.50 [−2.21, 0.77]; BF01 = 1.24, 9 studies, very low certainty of evidence). Regarding psychosocial outcomes, the direction of the effect was mainly inconsistent, and the certainty of the evidence was low to very low. Conclusions: Evidence is anecdotal according to Bayesian factors and uncertain whether perioperative psychological interventions may affect weight-related and psychosocial outcomes in people with clinically severe obesity undergoing bariatric surgery. As the results are ambiguous, we suggest conducting more high-quality studies in the field to estimate the true effect, its direction, and improve confidence in the body of evidence.
Postprandial Effects of Salmon Fishmeal and Whey on Metabolic Markers in Serum and Gene Expression in Liver Cells
Authors: Hjorth Marit , Galigniana Natalia M. , Ween Ola , Ulven Stine M. , Holven Kirsten B. , Dalen Knut Tomas , Sæther Thomas
Published: April 2022

Fish is considered an important part of a healthy diet, in part due to the content of long chain omega-3 fatty acids. However, both lean and fatty fish have beneficial health effects, suggesting that micronutrients and proteins may play a role. In a randomised, controlled, cross-over trial, five healthy male participants consumed 5.2 g of protein from either salmon fishmeal or whey. Blood samples were taken before and 30 and 60 min after intake. The concentration of glucose, lipids, hormones and metabolites, including 28 different amino acids and derivatives, were measured in serum or plasma. Cultured HepG2 cells were incubated with or without serum from the participants, and transcriptomic profiling was performed using RNA sequencing. The ingestion of both salmon fishmeal and whey reduced the glucose and triglyceride levels in serum. Protein intake, independent of the source, increased the concentration of 22 amino acids and derivatives in serum. Fishmeal increased the concentration of arginine, methionine, serine, glycine, cystathionine and 2-aminobutyric acid more than whey did. Incubation with postprandial serum resulted in large transcriptomic alterations in serum-fasted HepG2 cells, with the differential expression of >4500 protein coding genes. However, when comparing cells cultivated in fasting serum to postprandial serum after the ingestion of fishmeal and whey, we did not detect any differentially regulated genes, neither with respect to the protein source nor with respect to the time after the meal. The comparable nutrigenomic effects of fishmeal and whey do not change the relevance of fish by-products as an alternative food source.
The Inhibitory Effects of New Zealand Pine Bark (Enzogenol®) on α-Amylase, α-Glucosidase, and Dipeptidyl Peptidase-4 (DPP-4) Enzymes
Authors: Lim Wen Xin Janice , Gammon Cheryl S. , von Hurst Pamela , Chepulis Lynne , Page Rachel A.
Published: April 2022

The New Zealand pine bark extract (Enzogenol®) has previously been shown to elicit acute hypoglycaemic effects in humans. The present study investigated the underlying mechanisms of Enzogenol® in reducing postprandial glucose in humans. The potential inhibitory action of Enzogenol® against digestive enzymes: α-amylase and α-glucosidase, and dipeptidyl peptidase-4 (DPP-4) enzyme was determined. Enzogenol® demonstrated the ability to inhibit all three enzymes: α-amylase enzyme activity (IC50 3.98 ± 0.11 mg/mL), α-glucosidase enzyme activity (IC50 13.02 ± 0.28 μg/mL), and DPP-4 enzyme activity (IC50 2.51 ± 0.04 mg/mL). The present findings indicate the potential for Enzogenol® to improve postprandial glycaemia by delaying carbohydrate digestion via the inhibition of digestive enzymes (α-amylase and α-glucosidase), and enhancing the incretin effect via inhibiting the dipeptidyl-peptidase-4 enzyme. The inhibitory actions of Enzogenol® on enzymes should therefore be further validated in humans for its potential use in type 2 diabetes mellitus prevention and management.
Evaluating Dietary Patterns in Women from Southern Italy and Western Mexico
Authors: Ojeda-Granados Claudia , Barchitta Martina , La Rosa Maria Clara , La Mastra Claudia , Roman Sonia , Panduro Arturo , Agodi Antonella , Maugeri Andrea
Published: April 2022

Traditional diets are known to be beneficial; however, both Italian and Mexican populations are gradually moving away from the Mediterranean and traditional Mexican diets. Since women play a key role in safeguarding dietary traditions and may reflect population dietary changes, we aimed to identify Italian and Mexican women’s current dietary patterns (DPs) and characterize their nutrient content. Cross-sectional analyses were separately conducted on two convenience samples of 811 women from Southern Italy and 215 women from Western Mexico. Food frequency questionnaires, 24 h recalls, and a principal component analysis (PCA) approach were used to derive a posteriori DPs. In Italian women, the first DP was characterized by the consumption of legumes, vegetables, and fish (8.8% of the total variance), while the second DP was characterized by snack foods, processed meats, and non-olive oils. In Mexican women, the first DP was characterized by the consumption of meats and processed foods (12.6% of the total variance), while the second DP by fruits, vegetables, and whole grains. In both populations, adhering to the DPs rich in healthy foods (i.e., fruits, vegetables, legumes, and fish) was associated with a higher quality of diet in terms on nutrient content. However, adherence to the Western-type DPs was more common among women of younger age (p < 0.035). Thus, more extraordinary efforts are needed in promoting each country’s traditional healthy diet, especially among the new generations.
Exploratory Analysis of Selected Components of the mTOR Pathway Reveals Potentially Crucial Associations with Childhood Malnutrition
Authors: Palit Parag , Gazi Md Amran , Das Subhasish , Hasan Md Mehedi , Noor Zannatun , Ferdous Jafrin , Alam Md Ashraful , Nuzhat Sharika , Islam Md Ridwan , Mahfuz Mustafa , Haque Rashidul , Ahmed Tahmeed
Published: April 2022

Dysregulations in the mammalian target of rapamycin (mTOR) pathway are associated with several human anomalies. We aimed to elucidate possible implications for potential aberrations in the mTOR pathway with childhood malnutrition. We analyzed the activity of phospho-mTORC1 and the expressions of several mTOR pathway genes, namely: MTOR, TSC1, LAMTOR2, RPS6K1 and RICTOR from peripheral blood mononuclear cells isolated from venous blood of children suffering from different forms of malnutrition and compared them with those from healthy children. Significant reduction in the phosphorylation of mTORC1 was noted, as well as a decrease in expression of LAMTOR2 gene and increase in TSC1 gene expression were observed between malnourished children in comparison to the healthy children. The deregulation in the activity of the TSC1 and LAMTOR2 gene was significantly associated with all forms of childhood malnutrition. Our findings provide key insights into possible down-modulation in the overall activity of the mTOR pathway in childhood malnutrition. Further studies focusing on the analysis of a multitude of components involved in the mTOR pathway both at the gene and protein expression levels are required for conclusive evidence for the aforementioned proposition.
The Influence of Doing Shift Work on the Lifestyle Habits of Primary Care Nurses
Authors: Forcada-Parrilla Iris , Reig-Garcia Glòria , Serra Laura , Juvinyà-Canal Dolors
Published: April 2022

Nurses’ lifestyle habits play a key role in promoting healthy lifestyles; although, they may not always be entirely healthy and can be influenced by working conditions. This paper aims to analyze the influence of doing shift work on nurses’ lifestyle habits. Participants (n = 219) were recruited from 27 primary health care centres in Spain. Data were collected on socio-demographic characteristics, working conditions and lifestyle behaviour, assessed by use of an adhoc questionnaire including validated measures. Descriptive analysis and logistic regression models were performed. A total of 95% of the nurses reported having an adequate diet; 45.2% did not engage in any type of physical activity; and 85.8% did not smoke, especially women. A total of 60.3% did shift work, especially the younger ones (80.8%; p < 0.001), these nurses being the ones with the worst food habits (81.8%). In contrast, nurses who did shift work, exercised more days a week (69.5%; p < 0.001). The dietary habits of the nurses were adequate. Low tobacco consumption and low adherence to physical activity were observed, especially among women. Shift work harmed eating habits and obesity rates, but was a protective factor in terms of adherence to physical exercise.
Free Radical Properties, Source and Targets, Antioxidant Consumption and Health
Authors: Martemucci Giovanni , Costagliola Ciro , Mariano Michele , D’andrea Luca , Napolitano Pasquale , D’Alessandro Angela Gabriella
Published: April 2022

Free radicals have acquired growing importance in the fields of biology and medicine. They are produced during many different endogenous and exogenous processes. Mitochondria are the main source of endogenous reactive oxygen species (ROS) produced at cell level. The overproduction of free radicals can damage macromolecules such as nucleic acids, proteins and lipids. This leads to tissue damage in various chronic and degenerative diseases. Antioxidants play a crucial role in the body’s defense against free radicals. This review concerns the main properties of free radicals, their sources and deleterious effects. It highlights the potential role of the dietary supplementation of antioxidants and discusses unsolved problems regarding antioxidant supplements in the prevention and therapy of diseases.
Physiological Effects and Human Health Benefits of Hibiscus sabdariffa: A Review of Clinical Trials
Authors: Montalvo-González Efigenia , Villagrán Zuamí , González-Torres Sughey , Iñiguez-Muñoz Laura , Isiordia-Espinoza Mario , Ruvalcaba-Gómez José , Arteaga-Garibay Ramón , Acosta José , González-Silva Napoleón , Anaya-Esparza Luis
Published: April 2022

Hibiscus sabdariffa Linn. Malvaceae (HS) is characterized by its edible calyxes. The HS calyxes are widely used for cosmetic, food, and medicinal applications. According to ethnobotanical evidence, decoction, infusion, or maceration extracts from HS calyxes have been used in folk medicine to treat many ailments. Moreover, several in vitro and in vivo studies have demonstrated the pharmacological properties and potential human health benefits of HS consumption. On the other hand, the evaluation of the physiological effects and health benefits of HS in clinical studies is most challenging. Therefore, this narrative review summarizes and discusses the physiological effects and health benefits of HS calyxes reported in clinical trials. Preparations obtained from HS calyxes (extracts, infusions, decoction, teas, beverages, capsules, and pills) are used as non-pharmacological therapies to prevent/control diverse chronic non-communicable diseases. The most-reported HS health benefits are its antihypertensive, antidyslipidemic, hypoglycemic, body fat mass reduction, nephroprotective, antianemic, antioxidant, anti-inflammatory, and anti-xerostomic activities; these effects are associated with the phytochemicals found in HS. Moreover, no adverse effects were reported during the clinical trials. However, clinical studies exhibited some limitations; thus, further studies are required to validate the clinical efficacy of HS in large-scale studies with higher doses and a good experimental design
Development of a Lyophilized Off-the-Shelf Mesenchymal Stem Cell-Derived Acellular Therapeutic
Authors: Driscoll Julia , Yan Irene K. , Patel Tushar
Published: April 2022

The therapeutic activities elicited by mesenchymal stem cells (MSC) are in part mediated through paracrine action by the release of extracellular vesicles (EV) and secreted proteins. Collectively, these MSC-derived factors, referred to as the secretome product (SP), are intrinsically therapeutic and represent an attractive alternative to cell-based therapies. Herein, we developed a lyopreservation protocol to extend the shelf-life of the MSC-SP without compromising the structural or functional integrity of the vesicular components. The SP isolated from normoxia- and anoxia-exposed MSC elicited protective effects in an in vitro model of oxidative injury and the bioactivity was retained in the lyophilized samples. Three separate formulations of MSC-SP were isolated by tangential flow filtration using sucrose, trehalose, and mannitol as lyoprotectant agents. The MSC-SPs were lyophilized using a manifold protocol and the structural and functional integrity were assessed. The trehalose formulation of SP exhibited the highest EV and protein recovery after manifold-based lyophilization. To facilitate development as a therapeutic, a shelf lyophilization protocol was developed which markedly enhanced the recovery of EV and proteins. In conclusion, lyophilization represents an efficient method to preserve the structural and functional integrity of the MSC-SP and can be used to develop an off-the-shelf therapeutic.
A Novel Strategy to Reveal the Landscape of Crossovers in an F1 Hybrid Population of Populus deltoides and Populus simonii
Authors: Li Zhiting , Zhao Wei , Zhang Jinpeng , Pan Zhiliang , Bai Shengjun , Tong Chunfa
Published: April 2022

Although the crossover (CO) patterns of different species have been extensively investigated, little is known about the landscape of CO patterns in Populus because of its high heterozygosity and long-time generation. A novel strategy was proposed to reveal the difference of CO rate and interference between Populus deltoides and Populus simonii using their F1 hybrid population. We chose restriction site-associated DNA (RAD) tags that contained two SNPs, one only receiving the CO information from the female P. deltoides and the other from the male P. simonii. These RAD tags allowed us to investigate the CO patterns between the two outbred species, instead of using the traditional backcross populations in inbred lines. We found that the CO rate in P. deltoides was generally greater than that in P. simonii, and that the CO interference was a common phenomenon across the two genomes. The COs landscape of the different Populus species facilitates not only to understand the evolutionary mechanism for adaptability but also to rebuild the statistical model for precisely constructing genetic linkage maps that are critical in genome assembly in Populus. Additionally, the novel strategy could be applied in other outbred species for investigating the CO patterns.
Tomato Defense against Whiteflies under Drought Stress: Non-Additive Effects and Cultivar-Specific Responses
Authors: González-Klenner Francisca J. , Albornoz Marta V. , Ávila-Sákar Germán , Verdugo Jaime A.
Published: April 2022

Two of the main causes of losses in tomato production are the greenhouse whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae), and drought, which is becoming a central problem in agriculture due to global climate change. The separate effects of whitefly infestation and drought have been amply studied in many crop systems. However, less is known about their combined effects. To evaluate whether drought stress (DS) affects plant defense against whiteflies, we assessed the joint effects of whitefly infestation and DS on plant vegetative and reproductive performance in four tomato cultivars, and assessed the effects of DS on plant resistance and tolerance (compensatory ability) to whiteflies in a greenhouse experiment. Generally, we found negative effects of DS and whiteflies on plant performance, but the combined effects of DS and herbivory were not worse than those of either stress alone. In fact, plant performance under the combined effect of both stresses was usually similar to that in the presence of whiteflies without DS. Plants growing under DS had greater trichome density. However, plant resistance—as measured by whitefly population growth—decreased under DS in two cultivars and was unaffected in the other two. Compensatory ability decreased under DS in all but one cultivar. These cultivar-specific responses suggest genetic variation in resistance and tolerance to whiteflies and could be associated with differences in drought tolerance among cultivars. Our findings underscore the difficulty in predicting the combined effects of DS and herbivory and point to the need for a better understanding of the mechanisms underlying plant responses to both stresses at the molecular, cellular, and organismal levels.
Salicornia europaea L. Functional Traits Indicate Its Optimum Growth
Authors: Cárdenas-Pérez Stefany , Rajabi Dehnavi Ahmad , Leszczyński Karol , Lubińska-Mielińska Sandra , Ludwiczak Agnieszka , Piernik Agnieszka
Published: April 2022

Salicornia europaea L. grows in areas periodically flooded by salty or brackish water. It has potential economic value, because it can be used as food, forage, or biofuel, and has potential in pharmaceuticals and cosmetics. Increasing interest in S. europaea is due to its extreme salt tolerance and well growth in marginal saline soils. However, the variation in its functional traits in response to environmental conditions is still poorly studied. There are still questions regarding the optimal level of salinity for different traits. Therefore, we worked to address the question if S. europaea traits from different scales are controlled by salinity level. Based on performed pot experiment, we found that almost all traits are salinity dependent but affected in different ways. We demonstrated that morphological, biomass, and anatomical properties indicate optimum growth between 200 and 400 mM NaCl and growth limitations at 0, 800, and 1000 mM NaCl. Moreover, we found the most affected traits which include photosynthetic pigments and protein content, plant surface area, peroxidase activity, and anatomic traits related to cell shape. Our results significantly expanded the knowledge about S. europaea functional traits variation in response to salinity, which can be important for discovering regulating processes and for possible future agricultural applications.
Mechanical Properties and Dimensional Stability of Poplar Wood Modified by Pre-Compression and Post-Vacuum-Thermo Treatments
Authors: He Zaixin , Qi Yanran , Zhang Gang , Zhao Yueying , Dai Yong , Liu Baoxuan , Lian Chenglong , Dong Xiaoying , Li Yongfeng
Published: April 2022

Fast-growing poplar wood has the bottleneck problems of inferior mechanical strength and poor dimensional stability. In this study, the wood was modified by combined treatments of pre-compression and post-vacuum-thermo modification to improve its mechanical strength and dimensional stability, simultaneously; in addition, the variation law of mechanical properties of the wood with compression ratio as well as the improvement effect of dimensional stability of the treated wood were mainly studied. The results show that the optimal temperature and time of the vacuum-thermo modification were 190 °C and 10 h, respectively. Under these conditions, the structure of pre-compressed and post-vacuum-thermally modified wood (CT wood) is gradually densified with the increase in the compression ratio, which results in the continuous enhancement of mechanical properties. Meanwhile, the anti-swelling efficiency (ASE) of the CT wood after water absorption is correspondingly better than that of the compressed wood before thermal modification, indicating that the dimensional stability of compressed wood was improved by the thermal modification. When the compression ratio was 70%, the modulus of rupture (MOR) and impact toughness of CT wood was 176 MPa and 63 KJ/m2, which was 125% and 59% higher than that of untreated wood, respectively. The ASE was also 26% higher than that of the wood with sole compression. Therefore, this method improves the mechanical strength and dimensional stability of wood simultaneously, and it provides a scientific basis for optimization of the reinforcing modification process of fast-growing wood.
Multi-Band Polarization-Insensitive Metamaterial Absorber for Microwave Based on Slotted Structure and Magnetic Rubber
Authors: Li Kai , Lu Haipeng , Bi Mei , He Wentao , Qi Lun , Zhou Zhangrong , Weng Xiaolong
Published: April 2022

A design method of five-band polarization-insensitive metamaterial absorber (MMA) based on the slotted structures and the magnetic rubber is proposed for L-, S-, C-, X-, and Ku-band applications. The slotted structures of the top layer, which evolved from two square rings, are used to excite multi-resonance. The range of the electromagnetic (EM) parameters of a magnetic rubber substrate, which is used to adjust the equivalent impedance of the absorber to match the free space impedance in different bands, is estimated using the impedance matching principle. A series of magnetic rubber substrates based on the estimated EM parameters are prepared and measured, whose thickness is only 0.7 mm, meeting the thin design requirements. The absorption of the proposed absorber greater than 90% at 1.7 GHz, 3.87 GHz, 5.96 GHz, 9.4–10.4 GHz, and 14 GHz is achieved when the doping amount of the carbonyl iron powders is 200%. The absorbing performance of the absorber with measured EM parameter agrees well with the theoretical estimates, which validates the accuracy of the proposed design method.
Digital Twin Applications: A Survey of Recent Advances and Challenges
Authors: da Silva Mendonça Rafael , de Oliveira Lins Sidney , de Bessa Iury Valente , de Carvalho Ayres Florindo Antônio , de Medeiros Renan Landau Paiva , de Lucena Vicente Ferreira
Published: April 2022

Industry 4.0 integrates a series of emerging technologies, such as the Internet of Things (IoT), cyber-physical systems (CPS), cloud computing, and big data, and aims to improve operational efficiency and accelerate productivity inside the industrial environment. This article provides a series of information about the required structure to adopt Industry 4.0 approaches and a brief review of related concepts to finally identify challenges and research opportunities to envision the adoption of so-called digital twins. We want to pay attention to upgrading older systems aiming to provide the well-known advantages of Industry 4.0 to such legacy systems as reducing production costs, increasing efficiency, acquiring better robustness of equipment, and reaching advanced process connectivity.
Predictive Classifier for Cardiovascular Disease Based on Stacking Model Fusion
Authors: Liu Jimin , Dong Xueyu , Zhao Huiqi , Tian Yinhua
Published: April 2022

The etiology of cardiovascular disease is still an unsolved world problem, and high morbidity, disability, and mortality are the main characteristics of cardiovascular diseases. There is, therefore, a need for effective and rapid early prediction of likely outcomes in patients with cardiovascular disease using artificial intelligence (AI) techniques. The Internet of Things (IoT) is becoming a catalyst for enhancing the capabilities of AI applications. Data are collected through IoT sensors and analyzed and predicted using machine learning (ML). Existing traditional ML models do not handle data inequities well and have relatively low model prediction accuracy. To address this problem, considering the data observation mechanism and training methods of different algorithms, this paper proposes an ensemble framework based on stacking model fusion, from Support Vector Machines (SVM), K-Nearest Neighbor (KNN), Logistic Regression (LR), Random Forest (RF), Extra Tree (ET), Gradient Boosting Decision Tree (GBDT), XGBoost, LightGBM, CatBoost, and Multilayer Perceptron (MLP) (10 classifiers to select the optimal base learners). In order to avoid the overfitting phenomenon generated by the base learners, we use the Logistic Regression (LR) simple linear classifier as the meta learner. We validated the proposed algorithm using a fused Heart Dataset from several UCI machine learning repositories and another publicly available Heart Attack Dataset, and compared it with 10 single classifier models. The experimental results show that the proposed stacking classifier outperforms other classifiers in terms of accuracy and applicability.
Russian World and Ukrainian Autocephaly: Religious Narratives in Anti-Colonial Nationalism of Ukraine
Authors: Surzhko Harned Lena
Published: April 2022

The paper examines the role of religious narratives in the on-going Russo-Ukrainian conflict. The literature on religious nationalism offers several ways in which religion plays a role in national identity narratives. The strong connection between the Russian Orthodox Church (ROC) and the Russian state have been well-known. The narrative of the “chosen” nation and “third Rome” have fueled Russian neo-imperial national discourse of Russkii Mir (Russian World) which shapes Russian Foreign Policy in the “near abroad”. The Church is used as tool to shape and disseminate these narratives, as a means for justification of Russian aggression in Ukraine. This paper seeks to analyze the role of the religious narratives of Russia neo-colonial and post-colonial nationalism in Ukraine. It argues that Ukrainian religious nationalism, should it develop, will do so in response to the Russian actions driven by the ideological religious narrative. President Poroshenko’s decision to support the recognition of an autocephalous Orthodox Church of Ukraine (OCU) in 2018 was a valiant effort to aid in the construction of Ukraine’s anti-colonial religious national narrative. Prior to the Russian invasion, there seemed to be relatively weak public support for the religious nationalist narrative in Ukraine. The evidence shows that commitment to religious pluralism continues to be prevalent in Ukrainian society.