bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of a self-report measure to assess sleep satisfaction: Protocol for the Suffolk Sleep Index (SuSI)
Authors: Protogerou Cleo , Gladwell Valerie , Martin Colin
Published: October 2022

Good sleep is essential for health but there is no consensus on how to define and measure people’s understanding of good sleep. To date, people’s perceptions of a good night’s sleep have been, almost exclusively, conceptualized under the lens of sleep quality, which refers to objective characteristics of good sleep, such as such as ease and time needed to fall asleep, hours of sleep, and physical symptoms during sleep and upon awakening. A related, yet different construct, sleep satisfaction, refers to perceived positive affect about one’s sleep experience and has, to date, received little attention. This research focuses on sleep satisfaction, rather than sleep quality, and aims to develop a self-report measure to assess sleep satisfaction in an English adult population. As the measure will be developed in Suffolk, England, and its primary intended users are Suffolk community members, it is labelled the Suffolk Sleep Index (SuSI). The SuSI will draw from principles of community-based participatory research, following a seven-phase developmental process comprising literature review, interviews with Suffolk community members, synthesis of literature review and interview findings, pre-testing, pilot-testing, scale evaluation, and refinement. The present research will also investigate indices related to sleep satisfaction, including the community’s general health status, lifestyle factors and socio-economic status. The research will add to the limited, yet emerging body of research on perceived sleep satisfaction and its measurement. To our knowledge, a valid and reliable sleep satisfaction self-report measure has not been developed in the United Kingdom previously.
Learning to see after early and extended blindness: A scoping review
Authors: May Eloise , Arach Proscovia , Kishiki Elizabeth , Geneau Robert , Maehara Goro , Sukhai Mahadeo , Hamm Lisa M.
Published: October 2022

Purpose

If an individual has been blind since birth due to a treatable eye condition, ocular treatment is urgent. Even a brief period of visual deprivation can alter the development of the visual system. The goal of our structured scoping review was to understand how we might better support children with delayed access to ocular treatment for blinding conditions.

Method

We searched MEDLINE, Embase and Global Health for peer-reviewed publications that described the impact of early (within the first year) and extended (lasting at least 2 years) bilateral visual deprivation.

Results

Of 551 reports independently screened by two authors, 42 studies met our inclusion criteria. Synthesizing extracted data revealed several trends. The data suggests persistent deficits in visual acuity, contrast sensitivity, global motion, and visual-motor integration, and suspected concerns for understanding complex objects and faces. There is evidence for resilience in color perception, understanding of simple shapes, discriminating between a face and non-face, and the perception of biological motion. There is currently insufficient data about specific (re)habilitation strategies to update low vision services, but there are several insights to guide future research in this domain.

Conclusion

This summary will help guide the research and services provision to help children learn to see after early and extended blindness.
Trifoliate Orange-Related Rootstocks Enhance the Horticultural Performance of ‘Shamouti’ Sweet Orange under Humid Subtropical Condition
Authors: Carvalho Deived Uilian de , Junior Rui Pereira Leite , Yada Inês Fumiko Ubukata , Tazima Zuleide Hissano
Published: October 2022

The narrow genetic pool for both scions and rootstocks used in the Brazilian orchards makes the citrus industry vulnerable to biotic and abiotic threats. Orchard diversification by using different scion–rootstock combinations is an efficient measure to promote citrus protection, through increasing the level of genetic diversity. In this paper, we report the horticultural performance of the mid-season ‘Shamouti’ sweet orange grafted on five different rootstocks (‘Rangpur’ lime, ‘Swingle’ citrumelo, ‘C-13′ citrange, and ‘Cleopatra’ and ‘Sunki’ mandarins) in a long-term experiment (2007–2017) under the Brazilian humid subtropical condition. ‘Shamouti’ trees were assessed for vegetative growth, yield, and fruit quality. Additionally, a study was performed to estimate tree density and yield for new plantings. Trees grafted on ‘Swingle’ and ‘C-13′ rootstocks were less vigorous and more productive, with cumulative yields of >480 kg per tree, allowing high-density plantings (363–337 trees·ha−1). Trees on ‘Cleopatra’, ‘Sunki’, and ‘Rangpur’ were the most vigorous among the tested rootstocks, with tree heights > 4.20 m. However, they took longer to establish in the field, evidenced by their growth progress. These combinations also displayed the lowest tree density estimation (≤311 trees·ha−1). Trees on ‘Cleopatra’ exhibited the lowest cumulative yield (255 kg per tree). Although some significant differences were found for fruit quality, all rootstock combinations produced fruit of suitable quality, attending the commercial grading. Our findings evidence the potential of the trifoliate orange-related rootstocks ‘C-13′ and ‘Swingle’ to be used as promising rootstocks for ‘Shamouti’ cultivation in the humid subtropics, promoting genetic diversification and enhancing yield and tree density in new orchards.
Genome-Wide Transcriptome Analysis Reveals That Upregulated Expression of Aux/IAA Genes Is Associated with Defective Leaf Growth of the slf Mutant in Eggplant
Authors: Du Wenchao , Lu Yang , Luo Shuangxia , Yu Ping , Shen Jiajia , Wang Xing , Xuan Shuxin , Wang Yanhua , Zhao Jianjun , Li Na , Chen Xueping , Shen Shuxing
Published: October 2022

Leaf size is a crucial trait in eggplant breeding, as it influences photosynthesis, plant biomass and management. However, little is known about the molecular mechanism regulating leaf size in eggplant. This study reports a small leaf mutant (slf) generated with the mutagen ethyl methane sulfonate (EMS). The slf mutant showed restricted cell proliferation and an increased content of auxin. Transcriptome analysis revealed that several genes involved in auxin signaling are upregulated in slf. Exogenous application of auxinole, an auxin antagonist of TIR1/AFB receptors, repressed the expression of these genes and restored leaf growth of slf, suggesting that the small leaf size of slf is likely associated with auxin signaling. This study provides essential clues to unveil the molecular mechanism of leaf size regulation in eggplant.
Effects of Primary Processing and Pre-Salting of Baltic Herring on Contribution of Digestive Proteases in Marinating Process
Authors: Kamiński Patryk , Szymczak Mariusz , Szymczak Barbara
Published: October 2022

The low-technological quality of herring caught during the feeding season makes it impossible to achieve full ripeness of the meat in marinades. One solution may be to assist ripening using herring digestive tract proteases. Therefore, whole herring, headed herring and fillets were marinated for 2–14 days using the German (direct) and Danish (pre-salted) methods. The results showed that the mass of marinades from fillets was lower than from herring with intestines and correlated strongly with salt concentration in the Danish method, in contrast to the German method. Marinades from whole and headed herring had significantly higher trypsin, chymotrypsin, carboxypeptidase-A and cathepsin activities than marinated fillets. The herring marinated with viscera had 2–3 times higher non-protein nitrogen, peptide and amino acid fractions, as well as ripened 3 days faster than the marinated fillets. After 2 weeks of marinating, the fillets did not achieve full ripeness of the meat, unlike marinades made from whole and headed herring. The pre-salting stage in the Danish method significantly reduced cathepsin D activity by the tenth day of marinating, which was compensated by digestive proteases only in the case of whole or headed herring. The digestive proteases activity in the fillets was too low to achieve the same effect. Sensory evaluation of texture and hardness-TPA correlated strongly with several proteases in whole herring marinades, in contrast to a weak correlation with only one protease when marinating fillets. Marinating with intestines makes it possible to produce marinades faster, more efficiently and with higher sensory quality from herring of low-technological quality.
An Effective ELECTRA-Based Pipeline for Sentiment Analysis of Tourist Attraction Reviews
Authors: Fang Hui , Xu Ge , Long Yunfei , Tang Weimian
Published: October 2022

In the era of information explosion, it is difficult for people to decide on a tourist destination quickly. Online travel review texts provide valuable references and suggestions to assist in decision making. However, tourist attraction reviews are primarily informal and noisy. Most works in this field focus on shallow machine learning models or non-pretrained deep learning models. These approaches struggle to generate satisfactory classification results. To solve this issue, the paper proposes a pipeline model. In the first step of this paper, we preprocess tourist attraction reviews by performing stopword removal, special character removal, redundancy deletion and negation substitution to reduce noise. Then, we propose an ELECTRA (Efficiently Learning an Encoder that Classifies Token Replacements Accurately) classifier for sentiment analysis of tourist attraction review. Finally, we compare our pipeline model with several representative deep text classification models. Extensive experiments have demonstrated the effectiveness of our approach to sentiment analysis of tourist attraction reviews. We not only provide one high-quality dataset for tourist attraction reviews, but our work can also expand and promote the development of sentiment analysis in other domains.
Exposure Assessment of Air Pollution in Lungs
Authors: Paul Akshoy Ranjan , Jain Anuj , Saha Suvash C.
Published: October 2022

In this article, a comprehensive literature survey on air pollution and its effects on the human respiratory system is carried out. Based on the knowledge gaps, a computational assessment is proposed to find the impact of air quality on respiratory suspended particulate matter (RSPM) deposition in the human airways. A realistic 3D geometric model of the human airway was constructed to study the airflow characteristics and RSPM (PM2.5 and PM10) transport and deposition in it for normal and moderate inhalation patterns (corresponding to natural breathing) of air having an unhealthy air quality index (AQI). The results identify inertial impact as the primary mechanism of particle deposition in the human airways. They also reveals the significant differences in the deposition patterns of PM2.5 and PM10 in the right and left bronchial airways.
Blocking Periostin Prevented Development of Inflammation in Rhabdomyolysis-Induced Acute Kidney Injury Mice Model
Authors: Muratsu Jun , Sanada Fumihiro , Koibuchi Nobutaka , Shibata Kana , Katsuragi Naruto , Ikebe Shoji , Tsunetoshi Yasuo , Rakugi Hiromi , Morishita Ryuichi , Taniyama Yoshiaki
Published: October 2022

Background: Rhabdomyolysis is the collapse of damaged skeletal muscle and the leakage of muscle-cell contents, such as electrolytes, myoglobin, and other sarcoplasmic proteins, into the circulation. The glomeruli filtered these products, leading to acute kidney injury (AKI) through several mechanisms, such as intratubular obstruction secondary to protein precipitation. The prognosis is highly mutable and depends on the underlying complications and etiologies. New therapeutic plans to reduce AKI are now needed. Up to now, several cellular pathways, with the nuclear factor kappa beta (NF-kB), as well as the proinflammatory effects on epithelial and tubular epithelial cells, have been recognized as the major pathway for the initiation of the matrix-producing cells in AKI. Recently, it has been mentioned that periostin (POSTN), an extracellular matrix protein, is involved in the development of inflammation through the modulation of the NF-kB pathway. However, how POSTN develops the inflammation protection in AKI by rhabdomyolysis is uncertain. This study aimed to investigate the role of POSTN in a rhabdomyolysis mice model of AKI induced by an intramuscular injection of 50% glycerol. Methods: In vivo, we performed an intramuscular injection of 50% glycerol (5 mg/kg body weight) to make rhabdomyolysis-induced AKI. We examined the expression level of POSTN through the progression of AKI after glycerol intramuscular injection for C57BL/6J wildtype (WT) mice. We sacrificed mice at 72 h after glycerol injection. We made periostin-null mice to examine the role of POSTN in acute renal failure. The role of periostin was further examined through in vitro methods. The development of renal inflammation is linked with the NF-kB pathway. To examine the POSTN function, we administrated hemin (100 μM) on NIH-3T3 fibroblast cells, and the following signaling pathways were examined. Results: The expression of periostin was highly increased, peaking at about 72 h after glycerol injection. The expression of inflammation-associated mRNAs such as monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-a) and IL-6, and tubular injury score in H-E staining were more reduced in POSTN-null mice than WT mice at 72 h after glycerol injection. Conclusion: POSTN was highly expressed in the kidney through rhabdomyolysis and was a positive regulator of AKI. Targeting POSTN might propose a new therapeutic strategy against the development of acute renal failure.
Method for the Determination of Solvent Sorption of Polylactic Acid and the Effect of Essential Oils on the Sorption Properties
Authors: Virág Lilla , Bocsi Róbert , Pethő Dóra
Published: October 2022

The investigation of the adsorption and diffusion properties of polylactic acid (PLA) is very important, because during the application of the polymer, interactions can occur between the polymer and its environment that can affect its properties and application. The aim of our work was to investigate a method for determining the solvent sorption capacity of PLA and to investigate how different additives, such as essential oils, affect the solvent sorption properties of the polymer. Experiments were carried out to explore the correlation between the solvent uptake of two different types of PLA granules and the solubility parameter of the selected 5 essential oils (Melissa officinalis, Mentha piperita, Foeniculum vulgare, Majorana hortensi, Thymus vulgaris) for 3 solvents. It was observed, that application of essential oils was changed the solvent uptake of the granules differently. While one granule solvent uptake decreased on average by 2–3 wt.%, the other increased by a similar amount. The difference of sorption capacity of pure and essential oil containing solvent were between 20–190%. The specific essential oil uptake was highest in solutions with a concentration of 2.00 mg/mL, about 2.00 mg EO/g PLA. In alcoholic solutions we observed a relation between the solvent uptake of PLA and the solubility parameter of the relevant essential oil.
Nanocomposite-Based Electrode Structures for EEG Signal Acquisition
Authors: Vajravelu Ashok , Abdul Jamil Muhammad Mahadi Bin , Abd Wahab Mohd Helmy Bin , Wan Zaki Wan Suhaimizan Bin , Vinod Vibin Mammen , Ramasamy Palanisamy Karthik , Nageswara Rao Gousineyah
Published: October 2022

Objective: To fabricate a lightweight, breathable, comfortable, and able to contour to the curvilinear body shape, electrodes built on a flexible substrate are a significant growth in wearable health monitoring. This research aims to create a GNP/FE electrode-based EEG signal acquisition system that is both efficient and inexpensive. Methodology: Three distinct electrode concentrations were developed for EEG signal acquisition, three distinct electrode concentrations (1.5:1.5, 2:1, and 3:0). The high strength-to-weight ratio to form the tribofilm in the fabrication of the electrode will provide good efficiency. The EEG signal is first subjected to a wavelet transform, which serves as a preliminary analysis. The use of biopotential signals in wearable systems as biofeedback or control commands is expected to substantially impact point-of-care health monitoring systems, rehabilitation devices, human–computer/machine interfaces (HCI/HMI), and brain–computer interfaces (BCIs). The graphene oxide (GO), glycerol (GL), and polyvinyl alcohol (PVA) GO/GL/PVA plastic electrodes were measured and compared to that of a commercially available electrode using the biopic equipment. The GO/GL/PVA plastic electrode was able to detect EEG signals satisfactorily after being used for two months, demonstrating good conductivity and lower noise than the commercial electrode. The GO/GL/PVA nanocomposite mixture was put into the electrode mold as soon as it was ready and then rapidly chilled. Results: The quality of an acquired EEG signal could be measured in several ways including by its error percentage, correlation coefficient, and signal-to-noise ratio (SNR). The fabricated electrode yield detection ranged from 0.81 kPa−1 % to 34.90 kPa−1%. The performance was estimated up to the response of 54 ms. Linear heating at the rate of 40 °C per minute was implemented on the sample ranges from 0 °C to 240 °C. During the sample electrode testing in EEG signal analysis, it obtained low impedance with a good quality of signal acquisition when compared to a conventional wet type of electrode. Conclusions: A large database was frequently built from all of the simulated signals in MATLAB code. Through the experiment, all of the required data were collected, checked against all other signals, and proven that they were accurate representations of the intended database. Evidence suggests that graphene nanoplatelets (GNP) hematite (FE2O3) polyvinylidene fluoride (PVDF) GNP/FE2O3@PVDF electrodes with a 3:0 concentration yielded the best outcomes.
Constitution Method for Broadband Acoustic Metamaterials Based on the Design Theory of a One-Dimensional Distributed Transmission-Line Model
Authors: Nakagawa Tomoya , Nagayama Tsutomu , Fukushima Seiji , Watanabe Toshio
Published: October 2022

A method for realizing broadband acoustic metasurfaces composed of a one-dimensional distributed transmission-line model is proposed. There are no design formulas for determining the structural parameters of the structure constituting acoustic metasurfaces in the conventional method, and therefore parameter extractions by means of many calculations with numerical simulations are needed to realize acoustic metasurfaces. There are also narrow band operations or impedance matching problems. On the other hand, this paper shows that we can design broadband acoustic metasurfaces by determining the structural parameters with the design formulas of the model without many calculations. An acoustic metasurface that refracts incident plane waves at an angle of 20 degrees is first designed by using one-dimensional meander acoustic waveguide structures equivalent to the model, and these structural parameters are determined by the design formulas of the model and the modifications of the error from the theory. Full-wave simulations are performed, and the broadband operations and the validity of the design theory are shown from these results. Furthermore, a broadband acoustic flat lens is designed with the proposed structures as an example of the application of the proposed method, and these operations are also demonstrated by similar full-wave simulations.
The Impact of Depression on Quality of Life in Caregivers of Cancer Patients: A Moderated Mediation Model of Spousal Relationship and Caring Burden
Authors: Kim Yoonjoo
Published: October 2022

Family caregivers play an important role in managing and supporting cancer patients. Although depression in family caregivers is known to negatively affect caregiver health, the mechanism by which it affects caregivers is not clear. The purpose of this study was to explore the influence of depression on quality of life (QoL) in family caregivers of patients with cancer. Specifically, this study examined (1) whether caring burden mediates the relationship between depression and QoL, and (2) how this mediating effect varies depending on the caregiver’s relationship with the patient. This study performed a secondary analysis on cross-sectional survey data. Ninety-three family caregivers of cancer patients were included in the study. Moderated mediation analyses were conducted using PROCESS macro with the regression bootstrapping method. The moderated mediation models and the indirect effect of caregiver depression on QoL through caring burden were significantly different depending on caregivers’ relationships with patients (i.e., spousal or non-spousal). Specifically, the indirect effect of caregiver depression on QoL was greater for the patient’s spouse than for other family caregivers. Healthcare providers should focus on identifying caregivers’ depression and relationship with the patient and offer tailored support and intervention to mitigate the caring burden and improve the caregivers’ QoL.
Spatial Differences in Zooplankton Community Structure between Two Fluvial Lakes in the Middle and Lower Reaches of the Yangtze River: Effects of Land Use Patterns and Physicochemical Factors
Authors: Zhang Siyong , Lu Wenqin , Zhou Zhongze , Chen Wenwen
Published: October 2022

The zooplankton community composition in shallow lakes is influenced by numerous factors, such as environmental factors and the land use patterns around the lake. To investigate the interaction between the spatial differences in the zooplankton community structure, aquatic parameters, and land use patterns in the Lake Chen Yao complex (Lake Chen Yao and Lake Feng Sha), we assessed them in four seasons from October 2020 to August 2021. The results showed that the zooplankton density and biomass of Lake Chen Yao were higher than the latter. The results of Pearson correlation and RDA analysis revealed that electrical conductivity (EC), Chlorophyll a (Chl.a), dissolved oxygen (DO), and pH were the main environmental factors affecting the zooplankton community structure in the two lakes. The nutrient content of nitrogen (N) and phosphorus (P) were significantly higher in Lake Chen Yao, and there was a considerable relationship with the distribution of land use patterns around the two lakes. The land use patterns were the main reason for the difference in water quality and thus the spatial variation in the characteristics of the zooplankton communities in the two lakes.
Brain Imaging in Epilepsy-Focus on Diffusion-Weighted Imaging
Authors: Huang Tzu-Hsin , Lai Ming-Chi , Chen Yu-Shiue , Huang Chin-Wei
Published: October 2022

Epilepsy is a common neurological disorder; 1% of people worldwide have epilepsy. Differentiating epileptic seizures from other acute neurological disorders in a clinical setting can be challenging. Approximately one-third of patients have drug-resistant epilepsy that is not well controlled by current antiepileptic drug therapy. Surgical treatment is potentially curative if the epileptogenic focus is accurately localized. Diffusion-weighted imaging (DWI) is an advanced magnetic resonance imaging technique that is sensitive to the diffusion of water molecules and provides additional information on the microstructure of tissue. Qualitative and quantitative analysis of peri-ictal, postictal, and interictal diffusion images can aid the differential diagnosis of seizures and seizure foci localization. This review focused on the fundamentals of DWI and its associated techniques, such as apparent diffusion coefficient, diffusion tensor imaging, and tractography, as well as their impact on epilepsy in terms of differential diagnosis, epileptic foci determination, and prognosis prediction.
Ultra-Broadband Angular-Stable Reflective Linear to Cross Polarization Converter
Authors: Zhang Bianmei , Zhu Chenghui , Zhang Ran , Yang Xiaofan , Wang Ye , Liu Xiaoming
Published: October 2022

An ultra-broadband angular-stable reflective linear to cross polarization converter based on metasurface is developed. The unit cell of the converter is formed by a slant end-loaded H-shaped resonator. The slant arrangement is to create polarization conversion. The end-loaded stubs are useful for miniaturization and the slots are responsible for enlarging bandwidth. The simulated results show that the polarization conversion ratio of the proposed design is better than 90% in the range of 9.83–29.37 GHz, corresponding to a relative bandwidth of 99.69%. It is also demonstrated that the mean polarization conversion ratio is larger than 80% even though the incident angle reaches 40° for both x-polarized and y-polarized incidences. To validate the design, a prototype of the proposed structure is fabricated and measured. Satisfactory agreement has been observed between measurement and simulation. Compared with the designs in the literature, the developed converter exhibits good performance of high efficiency, ultra-broadband and angular stability. Potential applications can be expected in polarization-controlled devices, stealth surfaces, antennas, etc.
Porous Activated Carbons Derived from Coffee Waste for Use as Functional Separators in Lithium-Sulfur Batteries
Authors: Shin Jae-Hoon , Park Yu-Yeon , Moon Sang-Hyun , Kim Ji-Hwan , Jang Jae-Sung , Kim Sung-Beom , Lee Seong-Nam , Park Kyung-Won
Published: October 2022

A novel approach has been proposed for improving the performance of lithium-sulfur batteries (LSBs) with a carbon-based material as an interlayer between the cathode and separator. With this method, the cross-over of lithium polysulfides (LiPS) to the anode is suppressed, increasing reutilization of the sulfur cathode. In this study, activated carbons (ACs) were prepared using coffee waste as a carbon source and potassium hydroxide (KOH) as an activation agent at various reaction temperatures ranging from 500 to 800 °C. With the rise in heating temperature, the specific surface areas, micro-surface areas, and micro-pore volumes of the AC samples gradually increased. In particular, the AC sample prepared at 800 °C and used as a functional separator for LSB exhibited improved capacity and cycling performance while suppressing the LiPS shuttle effect.
Morpho-Physiological Strategies of Shorea leprosula Miq. and Shorea acuminata Dyer in Response to Light Intensity and Nutrient Amendments
Authors: Siti Nurfaeiza Abd Razak , Wan Juliana Wan Ahmad , Shamsul Khamis , Shukor Md. Nor
Published: October 2022

Successfully restoring degraded forest areas depends on seedlings adapting their growth to suit harsh environments. Hence, the requirements for seedlings’ growth need to be addressed before replanting degraded sites. The present study determines the effect of abiotic factors viz. light irradiance (8%, 30%, and 100%), nutrient addition (no fertiliser (NF), NPK, and vermicompost) on the growth performance and photosynthetic capacity of two dipterocarp species seedlings, Shorea leprosula Miq. and Shorea acuminata Dyer. The morphological characteristics assessed for growth performance comprised plant height, stem diameter, leaf count, leaf area, relative chlorophyll concentration, biomass, and root-to-shoot ratio. Li-Cor 6400 and 6800 were used to measure the leaf gas exchange traits, including photosynthetic rate (A), transpiration rate (E), intercellular CO2 concentration (Ci), stomatal conductance (gsw), and water-use efficiency (WUE). Our results demonstrated that different levels of light intensity and nutrient amendment significantly impacted plant-growth performance. Plants grown in 30% irradiance showed better growth performance in terms of relative height growth rate (RHGR), mean number of leaves, and leaf areas 41%, 24%, and 32% higher than the control. The A value was also higher in 30% irradiance, but no significant differences were observed between each level of light irradiance. The addition of vermicompost gave better growth for RHGR, relative diameter growth rate (RDGR), mean number of leaves, biomass, and relative chlorophyll concentrations 47%, 40%, 131%, 19%, and 27% higher than the control, respectively. However, the results obtained for photosynthetic parameters were contrary to growth performance. The photosynthesis rate (A) was higher (14.8%) in NPK compared to the control, and the other photosynthetic parameters did not differ significantly despite different nutrient amendments. In terms of species, S. leprosula has better growth performance and photosynthetic characteristics than S. acuminata in different light irradiance and nutrient amendments, thereby rendering S. leprosula the preferred rehabilitation species. Generally, nutrient addition of either NPK or vermicompost and 30% light irradiance gave better morphological and physiological growth for both species. The outcome of this study could provide a better understanding on the forest rehabilitation strategy to reduce the seedling-mortality rate, particularly for climax tree species.
Ferritin Heavy Chain Binds Peroxiredoxin 6 and Inhibits Cell Proliferation and Migration
Authors: Di Sanzo Maddalena , Cozzolino Flora , Battaglia Anna Martina , Aversa Ilenia , Monaco Vittoria , Sacco Alessandro , Biamonte Flavia , Palmieri Camillo , Procopio Francesca , Santamaria Gianluca , Ortuso Francesco , Pucci Piero , Monti Maria , Faniello Maria Concetta
Published: October 2022

The H Ferritin subunit (FTH1), as well as regulating the homeostasis of intracellular iron, is involved in complex pathways that might promote or inhibit carcinogenesis. This function may be mediated by its ability to interact with different molecules. To gain insight into the FTH1 interacting molecules, we analyzed its interactome in HEK293T cells. Fifty-one proteins have been identified, and among them, we focused our attention on a member of the peroxiredoxin family (PRDX6), an antioxidant enzyme that plays an important role in cell proliferation and in malignancy development. The FTH1/PRDX6 interaction was further supported by co-immunoprecipitation, in HEK293T and H460 cell lines and by means of computational methods. Next, we demonstrated that FTH1 could inhibit PRDX6-mediated proliferation and migration. Then, the results so far obtained suggested that the interaction between FTH1/PRDX6 in cancer cells might alter cell proliferation and migration, leading to a less invasive phenotype.
Isolation and Characterization of Two Novel Siphoviruses Novomoskovsk and Bolokhovo, Encoding Polysaccharide Depolymerases Active against Bacillus pumilus
Authors: Skorynina Anna , Koposova Olga , Kazantseva Olesya , Piligrimova Emma , Ryabova Natalya , Shadrin Andrey
Published: October 2022

This study describes two novel bacteriophages infecting members of the Bacillus pumilus group. Even though members of the group are not recognized as pathogenic, several strains belonging to the group have been reported to cause infectious diseases in plants, animals and humans. Bacillus pumilus group species are highly resistant to ultraviolet radiation and capable of forming biofilms, which complicates their eradication. Bacteriophages Novomoskovsk and Bolokhovo were isolated from soil samples. Genome sequencing and phylogenetic analysis revealed that the phages represent two new species of the genus Andromedavirus (class Caudoviricetes). The phages remained stable in a wide range of temperatures and pH values. A host range test showed that the phages specifically infect various strains of B. pumilus. The phages form clear plaques surrounded by halos. Both phages Novomoskovsk and Bolokhovo encode proteins with pectin lyase domains—Putative depolymerases. Obtained in a purified recombinant form, the proteins produced lysis zones on the lawn of a B. pumilus strain. This suggests that Novomoskovsk and Bolokhovo may be effective for the eradication of B. pumilus biofilms.
miRNA-Mediated Epigenetic Regulation of Treatment Response in RA Patients—A Systematic Review
Authors: Mucientes Arkaitz , Lisbona Jose Manuel , Mena-Vázquez Natalia , Ruiz-Limón Patricia , Manrique-Arija Sara , Fernández-Nebro Antonio
Published: October 2022

This study aimed to evaluate the role of microRNAs (miRNA) as biomarkers of treatment response in rheumatoid arthritis (RA) patients through a systematic review of the literature. The MEDLINE and Embase databases were searched for studies including RA-diagnosed patients treated with disease-modifying antirheumatic drugs (DMARDs) that identify miRNAs as response predictors. Review inclusion criteria were met by 10 studies. The main outcome of the study was the response to treatment, defined according to EULAR criteria. A total of 839 RA patients and 67 healthy donors were included in the selected studies. RA patients presented seropositivity for the rheumatoid factor of 74.7% and anti-citrullinated C-peptide antibodies of 63.6%. After revision, 15 miRNAs were described as treatment response biomarkers for methotrexate, anti-tumour necrosis factor (TNF), and rituximab. Among treatments, methotrexate presented the highest number of predictor miRNAs: miR-16, miR-22, miR-132, miR-146a and miR-155. The most polyvalent miRNAs were miR-146a, predicting response to methotrexate and anti-TNF, and miR-125b, which predicts response to infliximab and rituximab. Our data support the role of miRNAs as biomarkers of treatment response in RA and point to DMARDs modifying the miRNAs expression. Nevertheless, further studies are needed since a meta-analysis that allows definitive conclusions is not possible due to the lack of studies in this field.
Capability of Human Dendritic Cells Pulsed with Autologous Induced Pluripotent Stem Cell Lysate to Induce Cytotoxic T Lymphocytes against HLA-A33-Matched Cancer Cells
Authors: Nakazawa Tsutomu , Maeoka Ryosuke , Morimoto Takayuki , Matsuda Ryosuke , Nakamura Mitsutoshi , Nishimura Fumihiko , Yamada Shuichi , Nakagawa Ichiro , Park Young-Soo , Nakase Hiroyuki , Tsujimura Takahiro
Published: October 2022

Irradiated murine induced-pluripotent stem cells (iPSCs) elicit the antitumor response in vivo. However, it is unclear whether human iPSCs would elicit antitumor effects. In the present study, we investigated the capability of human iPSC lysate (iPSL)-pulsed dendritic cells (DCs) (iPSL/DCs) to induce cancer-responsive cytotoxic T lymphocytes (CTLs) in vitro. iPSCs and DCs were induced from peripheral blood mononuclear cells isolated from a human leukocyte antigen (HLA)-A33 homozygous donor. The iPSL was pulsed with immature DCs, which were then stimulated to allow full maturation. The activated DCs were co-cultured with autologous CTLs and their responses to SW48 colorectal carcinoma cells (HLA-A32/A33), T47D breast cancer cells (HLA-A33/A33), and T98G glioblastoma cells (HLA-A02/A02) were tested with enzyme-linked immunospot (ELISPOT) assays. Comprehensive gene expression analysis revealed that the established iPSCs shared numerous tumor-associated antigens with the SW48 and T47D cells. Immunofluorescent analysis demonstrated that the fluorescent-labeled iPSL was captured by the immature DCs within 2 h. iPSL/DCs induced sufficient CTL numbers in 3 weeks for ELISPOT assays, which revealed that the induced CTLs responded to SW48 and T47D cells. Human iPSL/DCs induced cancer-responsive CTLs on HLA-A33-matched cancer cells in vitro and could be a promising universal cancer vaccine for treating and preventing cancer.
Drimane Sesquiterpene Alcohols with Activity against Candida Yeast Obtained by Biotransformation with Cladosporium antarcticum
Authors: Cortez Nicole , Marín Víctor , Jiménez Verónica A. , Silva Víctor , Leyton Oscar , Cabrera-Pardo Jaime R. , Schmidt Bernd , Heydenreich Matthias , Burgos Viviana , Duran Paola , Paz Cristian
Published: October 2022

Fungal biotransformation is an attractive synthetic strategy to produce highly specific compounds with chemical functionality in regions of the carbon skeleton that are not easily activated by conventional organic chemistry methods. In this work, Cladosporium antarcticum isolated from sediments of Glacier Collins in Antarctica was used to obtain novel drimane sesquiterpenoids alcohols with activity against Candida yeast from drimendiol and epidrimendiol. These compounds were produced by the high-yield reduction of polygodial and isotadeonal with NaBH4 in methanol. Cladosporium antarcticum produced two major products from drimendiol, identified as 9α-hydroxydrimendiol (1, 41.4 mg, 19.4% yield) and 3β-hydroxydrimendiol (2, 74.8 mg, 35% yield), whereas the biotransformation of epidrimendiol yielded only one product, 9β-hydroxyepidrimendiol (3, 86.6 mg, 41.6% yield). The products were purified by column chromatography and their structure elucidated by NMR and MS. The antifungal activity of compounds 1–3 was analyzed against Candida albicans, C. krusei and C. parapsilosis, showing that compound 2 has a MIC lower than 15 µg/mL against the three-pathogenic yeast. In silico studies suggest that a possible mechanism of action for the novel compounds is the inhibition of the enzyme lanosterol 14α-demethylase, affecting the ergosterol synthesis.
Stem Cell Strategies in Promoting Neuronal Regeneration after Spinal Cord Injury: A Systematic Review
Authors: Bonosi Lapo , Silven Manikon Poullay , Biancardino Antonio Alessandro , Sciortino Andrea , Giammalva Giuseppe Roberto , Scerrati Alba , Sturiale Carmelo Lucio , Albanese Alessio , Tumbiolo Silvana , Visocchi Massimiliano , Iacopino Domenico Gerardo , Maugeri Rosario
Published: October 2022

Spinal cord injury (SCI) is a devastating condition with a significant medical and socioeconomic impact. To date, no effective treatment is available that can enable neuronal regeneration and recovery of function at the damaged level. This is thought to be due to scar formation, axonal degeneration and a strong inflammatory response inducing a loss of neurons followed by a cascade of events that leads to further spinal cord damage. Many experimental studies demonstrate the therapeutic effect of stem cells in SCI due to their ability to differentiate into neuronal cells and release neurotrophic factors. Therefore, it appears to be a valid strategy to use in the field of regenerative medicine. This review aims to provide an up-to-date summary of the current research status, challenges, and future directions for stem cell therapy in SCI models, providing an overview of this constantly evolving and promising field.
Advancements and Updates on Operative Techniques in Spinal Deformity
Authors: Ryvlin Jessica , De la Garza Ramos Rafael , Hamad Mousa K. , Yassari Reza
Published: October 2022

Spinal deformity involves a spectrum of abnormal spinal curvatures deviating from normal alignment [...]
Association between Barthel Index, Grip Strength, and Physical Activity Level at Admission and Prognosis in Community-Acquired Pneumonia: A Prospective Cohort Study
Authors: Ryrsø Camilla Koch , Hegelund Maria Hein , Dungu Arnold Matovu , Faurholt-Jepsen Daniel , Pedersen Bente Klarlund , Ritz Christian , Krogh-Madsen Rikke , Lindegaard Birgitte
Published: October 2022

Background: Impaired functional status is a risk factor for hospitalization in patients with community-acquired pneumonia (CAP). The aim was to determine the influence of functional status and physical activity level on severe outcomes, including length of stay, admission to the intensive care unit (ICU), readmission, and mortality in patients with CAP. Methods: A prospective cohort study among patients hospitalized with CAP. Functional status was assessed with the Barthel index and grip strength, and physical activity level was assessed using the international physical activity questionnaire. Linear regression was used to assess the association with length of stay, and logistic regression was used to assess the risk of severe outcomes. Results: Among 355 patients admitted with CAP, 18% had a low Barthel index (<80), 45% had a low grip strength, and 75% had a low physical activity level. Low Barthel index was associated with increased risk of ICU admission (OR 3.6, 95% CI 1.2–10.9), longer length of stay (27.9%, 95% CI 2.3–59.7%), readmission within 30, 90, and 180 days (OR 2.1–2.4, p < 0.05), and mortality within 90 and 180 days (OR 4.2–5.0, p < 0.05). Low grip strength was associated with increased risk of 90 days readmission (OR 1.6, 95% CI 1.0–2.6, p < 0.05) and mortality within 30, 90, and 180 days (OR 2.6–3.2, p < 0.05). Low physical activity level was associated with increased risk of readmission within 90 and 180 days (OR 1.8–2.1, p < 0.05) and mortality within 30, 90, and 180 days (OR 3.3–5.5, p < 0.05). Conclusions: Impaired functional status and low physical activity level were associated with a longer length of stay and increased risk of ICU admission, readmission, and mortality in patients hospitalized with CAP. Routine assessment of functional status and physical activity level in clinical care could enable early identification of individuals with excess risk for a poor prognosis. Trial registration: ClinicalTrials.gov, NCT03795662.
Galectin-1 and Galectin-9 Concentration in Maternal Serum: Implications in Pregnancies Complicated with Preterm Prelabor Rupture of Membranes
Authors: Boroń Dorota Grażyna , Świetlicki Aleksy , Potograbski Michał , Kurzawińska Grażyna , Wirstlein Przemysław , Boroń Daniel , Drews Krzysztof , Seremak-Mrozikiewicz Agnieszka
Published: October 2022

Preterm prelabor rupture of membranes (pPROM) accounts for nearly half of premature births. Although several risk factors have been identified, no markers allowing for effective prevention have been discovered. In this study, we investigated how the maternal serum levels of galectin-1 and galectin-9 change in patients with pPROM in comparison to uncomplicated pregnancies. A total of 75 patients were enrolled to both study and control group (37 vs. 38, respectively). The serum concentration of galectin-1 and galectin-9 were assayed in duplicate using an enzyme-linked immunoassay. All analyses were performed using PQ Stat v. 1.8.4 software. Galectin-1 levels were significantly higher in the controls (13.32 vs. 14.71 ng/mL, p = 0.02). Galectin-9 levels were similar in both groups (13.31 vs. 14.76 ng/mL, p = 0.30). Lower galectin levels were detected for early pPROM (before 32nd GW) in comparison to late pPROM and the controls (8.85 vs. 14.45 vs. 14.71 ng/mL, p = 0.0004). Similar trend was observed in galectin-9 levels, although no statistical significance was found (11.57 vs. 14.25 vs. 14.76 ng/mL, p = 0.26). Low galectin-1 maternal serum level is associated with the incidence of preterm prelabor rupture of membranes. Galectin-9 maternal serum levels were not significantly correlated with pPROM. However, in order to investigate gal-1 and gal-9 levels as potential, promising markers of pPROM, further clinical studies on larger groups are required.
Analysis of Monomer Release from Different Composite Resins after Bleaching by HPLC
Authors: Demirel Mehmet Gökberkkaan , Gönder Hakan Yasin , Tunçdemir Makbule Tuğba
Published: October 2022

(1) Background: This study aimed to examine the effect of bleaching agents on the release of triethylenae glycol dimethacrylate, 2-hydroxyethyl methacrylate, bisphenol A, urethane dimethacrylate, and bisphenol A-glycidyl methacrylate monomers, which are released from different composite resins, using the high-performance liquid chromatography (HPLC) method. (2) Methods: Ninety disc-shaped specimens were produced and immersed in artificial saliva. After different bleaching applications [office type bleaching (OB) and home type bleaching (HB)], the specimens were immersed in a 75 wt% ethanol/water solution, and the released monomers were analyzed by HPLC at predefined time intervals: 1, 7, and 28 days. The Kruskal–Wallis and Mann–Whitney U tests were conducted for statistical analysis (p = 0.05). (3) Results: The monomers were released at all times from all composite specimens. The monomer release was increased over time. The highest monomer release was detected on day 28. Bleaching applications affected monomer release. No statistical difference was found between OB and HB applications (p > 0.05). The most released monomer was Bisphenol-A in all composites. (4) Conclusion: Given that a residual monomer release from composite resins has a toxic effect and that bleaching treatments increase this release, a treatment protocol should be made in accordance with the manufacturer’s instructions.
Morphology and Anti-Corrosive Performance of Cr(III) Passivated Zn–Fe Alloy Coating on NdFeB Substrate
Authors: Li Ba , Zhou Xiaoshun , Chen Xiaoping , Fu Song , Wang Xiangdong , Zhao Dongliang
Published: October 2022

In this study, low-iron Zn–Fe alloy coatings and pure Zn coatings, with or without trivalent chromium passivation treatment, were electrodeposited onto a sintered NdFeB magnet from a weak acid chloride bath. The surface morphology and structure of the coatings were then examined using the X-ray diffraction, a scanning electron microscope and 3D white-light interfering surface analysis. Meanwhile, the electrodeposition behavior and anti-corrosive properties of the coatings were investigated using cyclic voltammetry, potentiodynamic polarization, electrochemical impedance spectroscopy, and natural salt spray tests. The results indicate that a passivated Zn–Fe alloy coating with a 0.9 wt.% Fe content provided much better corrosion resistance than a pure Zn coating and could provide both anodic protection and physical barrier function in the NdFeB substrates. The Fe element in Zn–Fe alloy coating was predominantly in solid solution in η-phase and small amounts in elemental form, which was beneficial to acquire a compact coating and passivation film. Finally, the passivated Zn–Fe alloy coating withstood 210 h against a neutral 3.5 wt.% NaCl salt spray without any white rust, which was 3–4 times longer than the pure Zn coating.
Dynamic Characterization of Hexagonal Microstructured Materials with Voids from Discrete and Continuum Models
Authors: Colatosti Marco , Shi Farui , Fantuzzi Nicholas , Trovalusci Patrizia
Published: October 2022

The mechanical response of materials such as fiber and particle composites, rocks, concrete, and granular materials, can be profoundly influenced by the existence of voids. The aim of the present work is to study the dynamic behavior of hexagonal microstructured composites with voids by using a discrete model and homogenizing materials, such as micropolar and classical Cauchy continua. Three kinds of hexagonal microstructures, named regular, hourglass, and skew, are considered with different length scales. The analysis of free vibration of a panel described as a discrete system, as a classical and as a micropolar continuum, and the comparison of results in terms of natural frequencies and modes show the advantage of the micropolar continuum in describing dynamic characteristics of orthotropic composites (i.e., regular and hourglass microstructures) with respect to the Cauchy continuum, which gives a higher error in frequency evaluations for all three hexagonal microstructured materials. Moreover, the micropolar model also satisfactorily predicts the behavior of skewed microstructured composites. Another advantage shown here by the micropolar continuum is that, like the discrete model, this continuum is able to present the scale effect of microstructures, while maintaining all the advantages of the field description. The effect of void size is also investigated and the results show that the first six frequencies of the current problem decrease by increasing in void size.