bluuebunny/crossref_metadata_2025 huggingface dataset:
The acute-to-chronic glycemic ratio correlates with the severity of illness at admission in patients with diabetes experiencing acute ischemic stroke
Authors: Liu Chao , Zhu Xu-ping , Zhu Xiao-wei , Jiang Yan-min , Xi Guang-jun , Xu Lan
Published: November 2022

Acute hyperglycemia is a powerful indicator of the severity of acute ischemic stroke (AIS); however, the relationship between these two factors is not very clear in patients with diabetes. We aimed to retrospectively evaluate data from 335 consecutive patients who experienced AIS from November 2015 to November 2016 to investigate whether a comprehensive assessment of blood glucose levels is a more valuable indicator of the severity of AIS or the presence of acute hyperglycemia in patients with diabetes. We collected demographic data, clinical manifestation information, clinical scores, and laboratory data [including fasting blood glucose and glycated hemoglobin (HbA1c) levels]. We estimated prehospital mean blood glucose concentrations using the following formula [1.59 * HbA1c (%) – 2.59] to calculate the “Acute-to-Chronic Glycemic Ratio” (AC ratio). The AC ratio differed significantly among patients grouped according to the National Institutes of Health Stroke Scale/Score (NIHSS) at admission (admission NIHSS) (p = 0.006). Univariate regression analysis revealed a correlation between the AC ratio and admission NIHSS [standardized β-coefficient (Std. B) = 0.164, p = 0.004]. The adjusted linear regression analysis revealed a correlation between both HbA1c (Std. B = 0.368, p = 0.038) and the AC ratio (Std. B = 0.262, p = 0.022) and admission NIHSS. The AC ratio (Std. B = 0.161, p = 0.012) was related to admission NIHSS in the stepwise variable selection. For an admission NIHHS > 4, the AC ratio (Std. B = 0.186, p = 0.047) was related to admission NIHSS in the stepwise variable selection. The AC ratio (Std. B = 1.163, p = 0.006 and Std. B = 0.565, p = 0.021, respectively) were related to admission NIHSS in both large-artery atherosclerosis (LAA) and small-vessel occlusion (SVO) subgroups. Thus, the AC ratio is related to admission NIHSS in patients with diabetes who experienced AIS and may be a better indicator of severity than acute blood glucose levels.
Case report: Covered stent placement to treat delayed aneurysmal rupture after flow diverter-assisted coil embolization
Authors: Chen Xiheng , Gui Siming , Dong Linggen , Zhang Longhui , Ge Huijian , Liu Peng , Li Youxiang , Lv Ming
Published: November 2022

Introduction

Flow diverter (FD) placement is widely accepted as a treatment for large saccular intracranial aneurysms. Delayed aneurysmal rupture (DAR) after FD placement is potentially catastrophic and difficult to treat. To our knowledge, using a Willis covered stent (WCS) to treat DAR after placement of a Pipeline Flex embolization device (PFED) combined with coiling has not been previously reported.

Case presentation

A 49-year-old woman with an incidental asymptomatic large right supraclinoid internal carotid artery aneurysm was treated with PFED placement and adjunctive coiling. DAR causing subarachnoid hemorrhage occurred 11 hours after the procedure. Treatment using a WCS was successful and resulted in a favorable clinical outcome (modified Rankin scale score 2).

Conclusion

DAR after FD implantation requires isolation of the aneurysm from the cerebral circulation as soon as possible. WCS placement can achieve this immediately and occlude the aneurysm. We hope our case could provide new idea for similar cases in the future.
Common predictors of spoken and written language performance in aphasia, alexia, and agraphia
Authors: Beeson Pélagie M. , Rising Kindle , Sachs Alyssa , Rapcsak Steven Z.
Published: November 2022

Language performance requires support from central cognitive/linguistic abilities as well as the more peripheral sensorimotor skills to plan and implement spoken and written communication. Both output modalities are vulnerable to impairment following damage to the language-dominant hemisphere, but much of the research to date has focused exclusively on spoken language. In this study we aimed to examine an integrated model of language processing that includes the common cognitive processes that support spoken and written language, as well as modality-specific skills. To do so, we evaluated spoken and written language performance from 87 individuals with acquired language impairment resulting from damage to left perisylvian cortical regions that collectively constitute the dorsal language pathway. Comprehensive behavioral assessment served to characterize the status of central and peripheral components of language processing in relation to neurotypical controls (n= 38). Performance data entered into principal components analyses (with or without control scores) consistently yielded a strong five-factor solution. In line with a primary systems framework, three central cognitive factors emerged: semantics, phonology, and orthography that were distinguished from peripheral processes supporting speech production and allographic skill for handwriting. The central phonology construct reflected performance on phonological awareness and manipulation tasks and showed the greatest deficit of all the derived factors. Importantly, this phonological construct was orthogonal to the speech production factor that reflected repetition of words/non-words. When entered into regression analyses, semantics and phonological skill were common predictors of language performance across spoken and written modalities. The speech production factor was also a strong, distinct predictor of spoken naming and oral reading, in contrast to allographic skills which only predicted written output. As expected, visual orthographic processing contributed more to written than spoken language tasks and reading/spelling performance was strongly reliant on phonological and semantic abilities. Despite the heterogeneity of this cohort regarding aphasia type and severity, the marked impairment of phonological skill was a unifying feature. These findings prompt greater attention to clinical assessment and potential treatment of underlying phonological skill in individuals with left perisylvian damage.
Thyroid hormone elicits intergenerational epigenetic effects on adult social behavior and fetal brain expression of autism susceptibility genes
Authors: Martinez Maria Elena , Stohn Julia Patrizia , Mutina Elizabeth M. , Whitten Rayne J. , Hernandez Arturo
Published: November 2022

Genetic mutations identified in genome-wide association studies can only explain a small percentage of the cases of complex, highly heritable human conditions, including neurological and neurodevelopmental disorders. This suggests that intergenerational epigenetic effects, possibly triggered by environmental circumstances, may contribute to their etiology. We previously described altered DNA methylation signatures in the sperm of mice that experienced developmental overexposure to thyroid hormones as a result of a genetic defect in hormone clearance (DIO3 deficiency). Here we studied fetal brain gene expression and adult social behavior in genetically normal F2 generation descendants of overexposed mice. The brain of F2 generation E13.5 fetuses exhibited abnormal expression of genes associated with autism in humans, including Auts2, Disc1, Ldlr, Per2, Shank3, Oxtr, Igf1, Foxg1, Cd38, Grid2, Nrxn3, and Reln. These abnormal gene expression profiles differed depending on the sex of the exposed ancestor. In the three-chamber social box test, adult F2 generation males manifested significantly decreased interest in social interaction and social novelty, as revealed by decrease total time, distance traveled and time immobile in the area of interaction with novel strangers. F1 generation mice, compared to appropriate controls also exhibited altered profiles in fetal brain gene expression, although these profiles were substantially different to those in the F2 generation. Likewise adult F1 generation mice showed some abnormalities in social behavior that were sexually dimorphic and milder than those in F2 generation mice. Our results indicate that developmental overexposure to thyroid hormone causes intergenerational epigenetic effects impacting social behavior and the expression of autism-related genes during early brain development. Our results open the possibility that altered thyroid hormone states, by eliciting changes in the epigenetic information of the germ line, contribute to the susceptibility and the missing—but heriTables—etiology of complex neurodevelopmental conditions characterized by social deficits, including autism and schizophrenia.
Clinical and genetic characteristics of Chinese Duchenne/Becker muscular dystrophy patients with small mutations
Authors: Gan Siyi , Liu Shulei , Yang Haiyan , Wu Liwen
Published: November 2022

Background

Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are amongst the inherited neuromuscular diseases with the highest incidence. Small mutations are less common and therefore have been poorly studied in China.

Materials and methods

The clinical data of 150 patients diagnosed with DMD/BMD by genetic analysis in Hunan Children’s Hospital from 2009 to 2021 were analyzed. The patients were followed up for an average of 3.42 years and their clinical characteristics were collected. Loss of ambulation (LOA) was used to evaluate the severity of disease progression. The correlation among clinical features, different variants, and glucocorticoid (GC) treatment was analyzed by Cox regression analysis.

Results

150 different variants were detected in this study, including 21 (14%) novel mutations, 88 (58.7%) non-sense mutations, 33 (22.0%) frameshift mutations, 22 (14.7%) splicing mutations, and 7 (4.7%) missense mutations. Single-exon skipping and single- or double-exon (double/single-exon) skipping strategies covered more than 90% of patients with small mutations. A case with frameshift mutation combined with Klinefelter’s syndrome (47, XXY) and another one with missense mutation combined with epilepsy was found in our study. De novo mutations accounted for 30.0% of all patients. The mean onset age was 4.19 ± 1.63 years old, and the mean diagnosed age was 5.60 ± 3.13 years old. The mean age of LOA was 10.4 years old (40 cases). 60.7% of them received GC treatment at 7.0 ± 2.7 years old. The main causes of complaints were muscle weakness, high creatine kinase (CK), motor retardation, and family history. The risk factors of LOA were positive family history (HR 5.52, CI 1.26–24.18), short GC treatment duration (HR 0.54, CI 0.36–0.82) and frameshift mutation (HR 14.58, CI 1.74–121.76). DMD patients who treated with GC after 7 years old had a higher risk of earlier LOA compared to those receiving treatment before the age of 7 (HR 0.083, CI 0.009–0.804). Moreover, an earlier onset age, a higher CK value, and a larger LOA population were found in the DMD patients compared to the BMD ones. Finally, the locations of the most frequent mutation were in exon 70 and exon 22.

Conclusion

In conclusion, 150 small mutations were identified in this study, and 21 of them were discovered for the first time. We found the hotspots of small mutations were in exon 70 and exon 20. Also, the analysis showed that positive family history, frameshift mutation, short duration of GC treatment, and delayed GC treatment resulted in earlier LOA for the DMD patients. Taken together, our findings complement the mutation spectrum of DMD/BMD, benefit us understanding to the DMD disease, and lay foundations for the clinical trials.
Genetic predisposition to neurodegenerative diseases and risk of stroke: A Mendelian randomization study
Authors: Li Fuju , Wang Yunyun , Hou Xiaoyan , Cao Lina , Zhou Xiaoyi , Yuan Weiyan , Shen Yi , Tian Tian
Published: November 2022

Background

Traditional epidemiological studies suggested that Neurodegenerative diseases (ND) might correlate with stroke. We intend to explore whether the two most common neurodegenerative diseases [Alzheimer’s disease (AD) and Parkinson’s disease (PD)] are causally associated with stroke and its subtypes.

Methods

Two-sample Mendelian Randomization (MR) method was used to explore the causal relationships. Candidate genetic instrumental variables (IVs) for AD and PD were collected from the genome-wide association studies (GWAS) in European populations. The inverse-variance weighted (IVW) method was the primary method of MR analysis, and the weighted median method was supplementary. In addition, the MR-Egger method and the MR-PRESSO test were used as well.

Results

We found no causal effects of AD on stroke, Ischemic stroke (IS), or Intracerebral hemorrhage (ICH). As for PD and stroke, our preliminary results showed PD could causally influence the risk of stroke [odds ratio (OR): 1.04; 95% confidence interval (CI): 1.02–1.07; P = 0.001 by the IVW method], although the alternative method did not support this result. We identified the positive causal relationship between PD and the risk of IS (OR = 1.04; 95% CI: 1.02–1.07; P = 0.001 by the IVW method), and the alternative MR methods produced similar results. The present study found there was no causal relationship between PD and ICH.

Conclusion

This study found a causal relationship between genetic susceptibility to PD and the incidence of stroke (especially IS) in the European population; however, there was no causal relation between AD and stroke risk.
Risk of dementia or Parkinson’s disease in the presence of Sjögren’s syndrome: A systematic review and meta-analysis
Authors: Wang Zhen-Zhi , Liu Meng-Si , Sun Zhen , Zhang Xu-Long , Zhang Mei-Ling , Xiong Kang , Zhou Feng
Published: November 2022

Objective

Evidence from observational studies suggests that Sjögren’s syndrome (SS) may contribute to an elevated risk of Parkinson’s disease (PD) and dementia. However, few studies have been undertaken to summarize and assess the consistency of the data quantitatively. Therefore, we evaluated the risk of dementia and PD in SS patients through a systematic review and meta-analysis approach.

Methods

Two reviewers independently conducted a systematic search of PubMed, Embase, and Web of Science databases (updated to February 14, 2022) to identify published literature on the association between SS and dementia or PD. The risk estimates of dementia or PD in patients with SS were pooled using fixed or random-effects models.

Results

Of the 631 studies initially searched, 10 were eventually included. Pooled results suggested that the risk of developing dementia significantly increased in patients with SS (HR = 1.24, 95% CI: 1.15–1.33, P < 0.001), and such risk in females with SS was similar to that in males. The risk of PD was 1.36 times higher in SS (HR = 1.36, 95% CI: 1.23–1.50, P < 0.001). The association between SS and PD risk appeared to occur primarily in female patients (female: HR = 1.28, 95% CI: 1.21–1.35; P < 0.001 vs. male: HR = 1.00, 95% CI: 0.87–1.16, P = 0.962, respectively). No significant effect of age was observed on the risk of developing PD and dementia in SS patients.

Conclusion

Our study supports that people with SS are at higher risk of PD and dementia than the general population. Further studies are needed to elucidate the underlying mechanisms and to assess whether interventions for SS have the potential to affect dementia and PD development.
Batch and sampling time exert a larger influence on the fungal community than gastrointestinal location in model animals: A meaningful case study
Authors: Li Jiayan , Chen Daiwen , Yu Bing , He Jun , Huang Zhiqing , Zheng Ping , Mao Xiangbing , Li Hua , Yu Jie , Luo Junqiu , Yan Hui , Luo Yuheng
Published: November 2022

Fungi play a fundamental role in the intestinal ecosystem and health, but our knowledge of fungal composition and distribution in the whole gastrointestinal tract (GIT) is very limited. The physiological similarity between humans and pigs in terms of digestive and associated metabolic processes places, the pig in a superior position over other non-primate models. Here, we aimed to characterize the diversity and composition of fungi in the GIT of pigs. Using high-throughput sequencing, we evaluated the fungal community in different locations of GIT of 11 pigs with 128.41 ± 1.25 kg body weight acquired successively. Among them, five pigs are sacrificed in April 2019 (Batch 1) and the other six are sacrificed in January 2020 (Batch 2). All subjects with similar genetic backgrounds, housing, management, and diet. Finally, no significant difference is found in the α-diversity (Richness) of the fungal community among all intestinal segments. Basidiomycota and Ascomycota are the two predominant fungal phyla, but Batch 1 harbored a notably high abundance of Basidiomycota and Batch 2 harbored a high abundance of Ascomycota. Moreover, the two batches harbored completely different fungal compositions and core fungal genera. FUNGuild (Fungal Functional Guild) analysis revealed that most of the fungal species present in the GIT are saprotroph, plant pathogen, and animal endosymbiont. Our study is the first to report that even under the same condition, large variations in fungal composition in the host GIT still occur from batch-to-batch and sampling time. The implications of our observations serve as references to the development of better models of the human gut.
Promoter hypomethylation and overexpression of TSTD1 mediate poor treatment response in breast cancer
Authors: Ansar Muhamad , Thu Le Thi Anh , Hung Chin-Sheng , Su Chih-Ming , Huang Man-Hsu , Liao Li-Min , Chung Yu-Mei , Lin Ruo-Kai
Published: November 2022

Epigenetic alterations play a pivotal role in cancer treatment outcomes. Using the methylation array data and The Cancer Genome Atlas (TCGA) dataset, we observed the hypomethylation and upregulation of thiosulfate sulfurtransferase–like domain containing 1 (TSTD1) in patients with breast cancer. We examined paired tissues from Taiwanese patients and observed that 65.09% and 68.25% of patients exhibited TSTD1 hypomethylation and overexpression, respectively. A significant correlation was found between TSTD1 hypomethylation and overexpression in Taiwanese (74.2%, p = 0.040) and Western (88.0%, p < 0.001) cohorts. High expression of TSTD1 protein was observed in 68.8% of Taiwanese and Korean breast cancer patients. Overexpression of TSTD1 in tumors of breast cancer patients was significantly associated with poor 5-year overall survival (p = 0.021) and poor chemotherapy response (p = 0.008). T47D cells treated with TSTD1 siRNA exhibited lower proliferation than the control group, and transfection of TSTD1 in MDA-MB-231 induced the growth of MDA-MB-231 cells compared to the vector control. Additionally, overexpression of TSTD1 in MCF7 cells mediated a poor response to chemotherapy by epirubicin (p < 0.001) and docetaxel (p < 0.001) and hormone therapy by tamoxifen (p =0.025). Circulating cell-free hypomethylated TSTD1 was detected in plasma of Taiwanese breast cancer patients with disease progression and poor chemotherapy efficacy. Our results indicate that promoter hypomethylation and overexpression of TSTD1 in patients with breast cancer are potential biomarkers for poor 5-year overall survival and poor treatment response.
Case Report: MAP2K1 K57N mutation is associated with primary resistance to anti-EGFR monoclonal antibodies in metastatic colorectal cancer
Authors: Mauri Gianluca , Patelli Giorgio , Gori Viviana , Lauricella Calogero , Mussolin Benedetta , Amatu Alessio , Bencardino Katia , Tosi Federica , Bonazzina Erica , Bonoldi Emanuela , Bardelli Alberto , Siena Salvatore , Sartore-Bianchi Andrea
Published: November 2022

Background

We aim to identify the prevalence and the role of the MAP2K1 K57N mutation in predicting resistance to anti-EGFR agents in metastatic colorectal cancer (mCRC) patients.

Methods

We retrospectively reviewed tumor-based next generation sequencing (NGS) results from mCRC patients screened for enrollment in the GO40872/STARTRK-2 clinical trial between July 2019 and March 2021. Then, in patients harboring microsatellite stable (MSS) RAS and BRAF wild-type MAP2K1 mutant mCRC, we reviewed outcome to treatment with anti-EGFR monoclonal antibodies.

Results

A total of 246 mCRC patients were screened. Most of them, 215/220 (97.7%), were diagnosed with MSS mCRC and 112/215 (52.1%) with MSS, RAS and BRAF wild-type mCRC. Among the latter, 2/112 (1.8%) had MAP2K1 K57N mutant mCRC and both received anti-EGFR monotherapy as third line treatment. In both patients, MAP2K1 K57N mutant tumors proved primary resistant to anti-EGFR agent panitumumab monotherapy. Of interest, one of these patients was treated with anti-EGFR agents three times throughout his course of treatment, achieving some clinical benefit only when associated with other cytotoxic agents (FOLFOX or irinotecan).

Conclusion

We verified in a clinical real-world setting that MAP2K1 K57N mutation is a resistance mechanism to anti-EGFR agents in mCRC. Thus, we suggest avoiding the administration of these drugs to MSS RAS and BRAF wild-type MAP2K1 N57K mutant mCRC.
Pediatric immune myelofibrosis (PedIMF) as a novel and distinct clinical pathological entity
Authors: Guerra Fabiola , L’Imperio Vincenzo , Bonanomi Sonia , Spinelli Marco , Coliva Tiziana Angela , Dell’Acqua Fabiola , Ferrari Giulia Maria , Corti Paola , Balduzzi Adriana , Biondi Andrea , Pagni Fabio , Saettini Francesco
Published: November 2022

Myelofibrosis is a rare myeloproliferative disorder. The detailed descriptions of myelofibrosis in children and adolescents is limited to a few case series and case reports describing fewer than 100 patients, thus suggesting the extreme rarity of this condition prior to adulthood. Though pediatric patients rarely present the typical features and outcomes usually observed in older people, pediatric myelofibrosis is not considered an independent entity. Here we aim to describe patients with pediatric myelofibrosis, showing different clinical and pathological features when compared to the World Health Organization 2016 Primary Myelofibrosis classification. We retrospectively collected and analyzed 14 consecutive pediatric myelofibrosis diagnosed in our Pediatric hematology outpatient clinic over a six-year period. According to clinical data and bone marrow biopsy findings, patients were classified into three subgroups: adult-like myelofibrosis, pediatric immune myelofibrosis, idiopathic myelofibrosis. Pediatric Immune Myelofibrosis was the predominant subgroup in our cohort (7/14). Pediatric Immune Myelofibrosis is characterized by peculiar bone marrow features (i.e., T lymphocyte infiltration) and a milder course compared to the other patients Pediatric Immune Myelofibrosis is a novel and distinct pathological entity. We suggest to carefully consider Pediatric Immune Myelofibrosis in case of bone marrow biopsies showing myelofibrosis that do not fulfill WHO criteria.
Construal level theory in the design of informational systems
Authors: McDermott Tom , Folds Dennis
Published: November 2022

In the context of human-machine teaming, we are observing new kinds of automated and “intelligent” applications that effectively model and manage both producer and consumer aspects of information presentation. Information produced by the application can be easily accessed by the user at multiple levels of abstraction, depending on the user’s current context and necessity. The research described in this article applies this concept of information abstraction to complex command and control systems in which distributed autonomous systems are managed by multiple human teams. We explore three multidisciplinary and foundational concepts that can be used to design information flow in human-machine teaming situations: 1) formalizing a language we call “RECITAL” (Rules of Engagement, Commander’s Intent, and Transfer of Authority Language), which defines the information flow based on concepts of intent, rules, and delegated authority; 2) applying this language to well-established models of human-machine distributed teams represented as a systemic control hierarchy; and 3) applying construal level theory from social psychology as a means to guide the producer-consumer model of the information abstractions. All three of these are integrated into a novel user interface concept designed to make information available to both human and machine actors based on task-oriented decision criteria. In this research, we describe a conceptual model for future information design to inform shared control and decision-making across distributed human and machine teams. We describe the theoretical components of the concepts and present the conceptual approach to designing such systems. Using the concept, we describe a prototype user interface to situationally manage the information in a mission application.
Multimodal learning for fetal distress diagnosis using a multimodal medical information fusion framework
Authors: Zhang Yefei , Deng Yanjun , Zhou Zhixin , Zhang Xianfei , Jiao Pengfei , Zhao Zhidong
Published: November 2022

Cardiotocography (CTG) monitoring is an important medical diagnostic tool for fetal well-being evaluation in late pregnancy. In this regard, intelligent CTG classification based on Fetal Heart Rate (FHR) signals is a challenging research area that can assist obstetricians in making clinical decisions, thereby improving the efficiency and accuracy of pregnancy management. Most existing methods focus on one specific modality, that is, they only detect one type of modality and inevitably have limitations such as incomplete or redundant source domain feature extraction, and poor repeatability. This study focuses on modeling multimodal learning for Fetal Distress Diagnosis (FDD); however, exists three major challenges: unaligned multimodalities; failure to learn and fuse the causality and inclusion between multimodal biomedical data; modality sensitivity, that is, difficulty in implementing a task in the absence of modalities. To address these three issues, we propose a Multimodal Medical Information Fusion framework named MMIF, where the Category Constrained-Parallel ViT model (CCPViT) was first proposed to explore multimodal learning tasks and address the misalignment between multimodalities. Based on CCPViT, a cross-attention-based image-text joint component is introduced to establish a Multimodal Representation Alignment Network model (MRAN), explore the deep-level interactive representation between cross-modal data, and assist multimodal learning. Furthermore, we designed a simple-structured FDD test model based on the highly modal alignment MMIF, realizing task delegation from multimodal model training (image and text) to unimodal pathological diagnosis (image). Extensive experiments, including model parameter sensitivity analysis, cross-modal alignment assessment, and pathological diagnostic accuracy evaluation, were conducted to show our models’ superior performance and effectiveness.
Long-term water use efficiency and non-structural carbohydrates of dominant tree species in response to nitrogen and water additions in a warm temperate forest
Authors: Jiang Xiyan , Song Mengya , Qiao Yaqi , Liu Mengzhou , Ma Lei , Fu Shenglei
Published: November 2022

Nitrogen (N) deposition tends to accompany precipitation in temperate forests, and vegetation productivity is mostly controlled by water and N availability. Many studies showed that tree species response to precipitation or N deposition alone influences, while the N deposition and precipitation interactive effects on the traits of tree physiology, especially in non-structural carbohydrates (NSCs) and long-term water use efficiency (WUE), are still unclear. In this study, we measured carbon stable isotope (δ13C), total soluble sugar and starch content, total phenols, and other physiological traits (e.g., leaf C:N:P stoichiometry, lignin, and cellulose content) of two dominant tree species (Quercus variabilis Blume and Liquidambar formosana Hance) under canopy-simulated N deposition and precipitation addition to analyze the changes of long-term WUE and NSC contents and to explain the response strategies of dominant trees to abiotic environmental changes. This study showed that N deposition decreased the root NSC concentrations of L. formosana and the leaf lignin content of Q. variabilis. The increased precipitation showed a negative effect on specific leaf area (SLA) and a positive effect on leaf WUE of Q. variabilis, while it increased the leaf C and N content and decreased the leaf cellulose content of L. formosana. The nitrogen–water interaction reduced the leaf lignin and total phenol content of Q. variabilis and decreased the leaf total phenol content of L. formosana, but it increased the leaf C and N content of L. formosana. Moreover, the response of L. formosana to the nitrogen–water interaction was greater than that of Q. variabilis, highlighting the differences between the two dominant tree species. The results showed that N deposition and precipitation obviously affected the tree growth strategies by affecting the NSC contents and long-term WUE. Canopy-simulated N deposition and precipitation provide a new insight into the effect of the nitrogen–water interaction on tree growth traits in a temperate forest ecosystem, enabling a better prediction of the response of dominant tree species to global change.
Molecular manipulations of miR398 increase rice grain yield under different conditions
Authors: Lu Yuzhu , Yao Kena , Gong Zhiyun , Zhang Yixin , Meng Yunlong , Liu Qiaoquan
Published: November 2022

Rice miR398 targets two stress-tolerant genes, CSD1-2 (Cu/Zn Superoxide Dismutases1-2) and CCS (copper chaperone of CSD), which usually boost plants’ tolerance by inhibiting growth. So, how to accurately regulate the activities of miR398 targets and thus make rice better able to adapt to different conditions has great significances in producing rice yields under the current circumstances of shrinking arable lands resulting from global urbanization and increasing salty soil caused by irrigation. Through controlling the expressions of miR398 in different levels, we found down-regulated expression of miR398 targets can promote growth under good growth conditions while up-regulated expressions of the targets can help rice tolerate salt. In this study, we over-expressed miR398 highly, moderately, and lowly, then three concomitantly inverse levels of its targets’ expression were obtained. Under normal growth conditions, the transgenic lines with low and moderate levels of over-expressions of miR398 could increase grain yields 14.5% and 7.3%, respectively, although no transgenic lines could survive well under salty conditions simulating real saline-alkali soil. Using short tandem target mimic (STTM) technology to silence miR398 highly, moderately, and lowly respectively, also three inverse levels of its targets’ expression were obtained. All three transgenic lines exhibited good agronomic performances under salt stress in inverse to their degrees of STTM, but their growth was inhibited differently under normal conditions. Altogether, we suggest that flexibly manipulating the expression of miR398 is an ideal strategy to help rice survive better and achieve optimized yields under specific conditions.
Genome-wide association studies of five free amino acid levels in rice
Authors: He Liqiang , Wang Huixian , Sui Yao , Miao Yuanyuan , Jin Cheng , Luo Jie
Published: November 2022

Rice (Oryza sativa L.) is one of the important staple foods for human consumption and livestock use. As a complex quality trait, free amino acid (FAA) content in rice is of nutritional importance. To dissect the genetic mechanism of FAA level, five amino acids’ (Val, Leu, Ile, Arg, and Trp) content and 4,325,832 high-quality SNPs of 448 rice accessions were used to conduct genome-wide association studies (GWAS) with nine different methods. Of these methods, one single-locus method (GEMMA), seven multi-locus methods (mrMLM, pLARmEB, FASTmrEMMA, pKWmEB, FASTmrMLM, ISIS EM-BLASSO, and FarmCPU), and the recent released 3VmrMLM were adopted for methodological comparison of quantitative trait nucleotide (QTN) detection and identification of stable quantitative trait nucleotide loci (QTLs). As a result, 987 QTNs were identified by eight multi-locus GWAS methods; FASTmrEMMA detected the most QTNs (245), followed by 3VmrMLM (160), and GEMMA detected the least QTNs (0). Among 88 stable QTLs identified by the above methods, 3VmrMLM has some advantages, such as the most common QTNs, the highest LOD score, and the highest proportion of all detected stable QTLs. Around these stable QTLs, candidate genes were found in the GO classification to be involved in the primary metabolic process, biosynthetic process, and catalytic activity, and shown in KEGG analysis to have participated in metabolic pathways, biosynthesis of amino acids, and tryptophan metabolism. Natural variations of candidate genes resulting in the content alteration of five FAAs were identified in this association panel. In addition, 95 QTN-by-environment interactions (QEIs) of five FAA levels were detected by 3VmrMLM only. GO classification showed that the candidate genes got involved in the primary metabolic process, transport, and catalytic activity. Candidate genes of QEIs played important roles in valine, leucine, and isoleucine degradation (QEI_09_03978551 and candidate gene LOC_Os09g07830 in the Leu dataset), tryptophan metabolism (QEI_01_00617184 and candidate gene LOC_Os01g02020 in the Trp dataset), and glutathione metabolism (QEI_12_09153839 and candidate gene LOC_Os12g16200 in the Arg dataset) pathways through KEGG analysis. As an alternative of the multi-locus GWAS method, these findings suggested that the application of 3VmrMLM may provide new insights into better understanding FAA accumulation and facilitate the molecular breeding of rice with high FAA level.
Physiological effects of γ-aminobutyric acid application on cold tolerance in Medicago ruthenica
Authors: Li Ying , Yu Xiaojun , Ma Kaikai
Published: November 2022

Low temperatures in the seedling stage during early spring limit Medicago ruthenica germination and seedling growth. Elucidating the physiological mechanism of γ-aminobutyric acid (GABA)-regulated cold tolerance in M. ruthenica could provide a reference for alleviating the harmful effects of low temperatures on legumes in alpine meadows. The regulatory effects of GABA on M. ruthenica physiological parameters were explored by simulating the ground temperatures in the alpine meadow area of Tianzhu, China, in early May (2 h at 7°C; 6 h at 15°C; 4 h at 12°C; 2 h at 7°C; 10 h at 3°C). Our results showed that 15 mmol/l GABA was the optimal spray concentration to promote growth in the aboveground and belowground parts and increase the fresh and dry weights of seedlings. At this concentration, GABA enhanced the activities of catalase, peroxidase, superoxide dismutase, and ascorbate peroxidase; increased the osmotic balance; and inhibited the production of harmful substances in the cells under low-temperature conditions. GABA also regulated the tissue structure of leaves, increased the cell tense ratio, maintained photochemical activity, increased the amount of light energy to the photochemical reaction center, and improved the photosynthetic rate. Furthermore, exogenous GABA application increased the endogenous GABA content by promoting GABA synthesis in the early stages of low-temperature stress but mainly participated in low-temperature stress mitigation via GABA degradation in the late stages. Our results show that GABA can improve the cold tolerance of M. ruthenica by promoting endogenous GABA metabolism, protecting the membrane system, and improving the leaf structure.
Effects of exergames on mood and cognition in healthy older adults: A randomized pilot study
Authors: Moret Beatrice , Nucci Massimo , Campana Gianluca
Published: November 2022

The elderly population is increasing and the implementation of stimulating training to promote active aging has become a research issue. This study aimed at investigating the effects of a cognitive-motor exergame training on cognitive functions and mood, in healthy older adults. A randomized controlled pilot study was conducted to compare a cognitive-motor exergame training with a passive control group. The training consisted of 8 sessions of 45 min each, including 10 interactive activities focused on several cognitive functions such as memory, processing speed and executive functions, all requiring motor planning and execution. A total of 57 participants were administered a battery of cognitive tests before and after the training. A mixed-effect ANOVA with group (experimental vs. control) as between factor and time (pre-and post-test) as within factor, was performed to evaluate the effect of the exergame training on cognitive abilities and mood. Results showed significant interaction effects in processing speed [STROOPC: F(1,53.4) = 9.04,p = 0.004,R2 = 0.82], inhibition [3backs’ false alarms: F(1,47.5) = 5.5,p = 0.02,R2 = 0.79], and mood [Beck Depression Inventory: F(1,55) = 4.15,p = 0.04,R2 = 0.6]. Even though post-hoc analyses did not provide statistical evidence supporting the interactions, overall data showed a trend toward better scores only for the experimental group, suggesting a potential improvement in information processing speed, working memory and mood. Exergaming may be a motivating and enjoyable approach to healthy and active aging.
Norms, prices, and commitment: A comprehensive overview of field experiments in the energy domain and treatment effect moderators
Authors: Vesely Stepan , Klöckner Christian A. , Carrus Giuseppe , Tiberio Lorenza , Caffaro Federica , Biresselioglu Mehmet Efe , Kollmann Andrea C. , Sinea Anca C.
Published: November 2022

This paper provides a comprehensive overview of field experiments utilizing social norms, commitment and price-based interventions to promote energy conservation, load shifting, and energy efficiency behaviors. Treatment effects reported in the extant literature, as well as the factors that may strengthen or dampen these effects are reviewed. We find that social norm and incentive-based interventions mostly achieve small reductions in energy consumption, and that the effects of commitment-based interventions are essentially zero for the most part. Incentive effects on energy efficiency investments are mostly non-existent, safe for a few exceptions. One gap that we identify is the almost complete absence of field experiments leveraging social norms or commitment to promote energy efficiency investments. We discuss a broad range of (mostly under-researched) plausible moderators of the interventions' effects. Crucially, a more careful attention to moderators in future research can highlight instances in which interventions can be effective, notwithstanding their modest or non-existent average treatment effects. Our review offers a starting point in this regard.
Experience of nature and times of silence as a resource to cope with the COVID-19 pandemic and their effects on psychological wellbeing—Findings from a continuous cross-sectional survey in Germany
Authors: Büssing Arndt , Recchia Daniela Rodrigues , Baumann Klaus
Published: November 2022

Background

The COVID-19 pandemic with its lockdowns affected social relations and mental health conditions of people worldwide. We aimed to analyze the relevance of nature and times of silence as resources to cope with the pandemic. Of interest were how experiences of nature and times of silence are related to the perception of wondering awe and gratitude and psychological wellbeing and how these have changed during the different phases of the pandemic. Finally, we asked whether Nature/Silence would mediate the link between Awe/Gratitude and wellbeing.

Methods

A cross-sectional survey with standardized questionnaires (i.e., PCQ, GrAw-7, BMLSS-10, WHO-5) enrolling participants during the different phases of the COVID-19 pandemic was conducted. The total sample of 5,155 participants from Germany consisted of 65% women and 34% men, with a mean age of 45.0 ± 14.0 years.

Results

Directly after the first lockdown, Nature/Silence and Awe/Gratitude scores were high and decreased along with wellbeing with the onset of the second lockdown in winter 2020, while perceived burden constantly increased. Nature/Silence was rated lowest by people with reduced wellbeing (eta2 = 0.058) and feeling lonely or socially isolated (eta2 = 0.042). Predictor analyses revealed that wellbeing as a dependent variable was predicted best by corona-related perception of burden, Awe/Gratitude, reflection of life, and Nature/Silence and further by perceived changes in terms of relationships and spirituality (R2 = 0.55). In mediation analyses, Awe/Gratitude proved to be a significant predictor for Nature/Silence (β = 0.55, p< 0.0001) and wellbeing (β = 0.05, p < 0.0001). The mediation analysis explained 37% of the variability in the data. The direct influence of Awe/Gratitude on wellbeing was estimated as β = 0.09 (p < 0.0001), and the mediation effect of Nature/Silence on the link between Awe/Gratitude and wellbeing was significant, too (β = 0.03, p < 0.0001), explaining 25% of the total effect.

Conclusion

Nature/Silence and Awe/Gratitude were used as relevant resources during the pandemic, although they cannot fully buffer the negative effects of the social restrictions that resulted in decreases in wellbeing and increases in perceived burden. Perception of nature as a sensitizer of positive experiences particularly during difficult phases of life could be trained to stabilize wellbeing and thus to contribute to public health.
Changing trends of patient characteristics and treatment pathways during the COVID-19 pandemic: A cross-sectional analysis of 72,459 inpatient cases from the German Helios database
Authors: König Sebastian , Hohenstein Sven , Pellissier Vincent , Leiner Johannes , Hindricks Gerhard , Nachtigall Irit , Kuhlen Ralf , Bollmann Andreas
Published: November 2022

Background

This study compared patient profiles and clinical courses of SARS-CoV-2 infected inpatients over different pandemic periods.

Methods

In a retrospective cross-sectional analysis, we examined administrative data of German Helios hospitals using ICD-10-codes at discharge. Inpatient cases with SARS-CoV-2 infection admitted between 03/04/2020 and 07/19/2022 were included irrespective of the reason for hospitalization. All endpoints were timely assigned to admission date for trend analysis. The first pandemic wave was defined by change points in time-series of incident daily infections and compared with different later pandemic phases according to virus type predominance.

Results

We included 72,459 inpatient cases. Patients hospitalized during the first pandemic wave (03/04/2020–05/05/2020; n = 1,803) were older (68.5 ± 17.2 vs. 64.4 ± 22.6 years, p < 0.01) and severe acute respiratory infections were more prevalent (85.2 vs. 53.3%, p < 0.01). No differences were observed with respect to distribution of sex, but comorbidity burden was higher in the first pandemic wave. The risk of receiving intensive care therapy was reduced in all later pandemic phases as was in-hospital mortality when compared to the first pandemic wave. Trend analysis revealed declines of mean age and Elixhauser comorbidity index over time as well as a decline of the utilization of intensive care therapy, mechanical ventilation and in-hospital mortality.

Conclusion

Characteristics and outcomes of inpatients with SARS-CoV-2 infection changed throughout the observational period. An ongoing evaluation of trends and care pathways will allow for the assessment of future demands.
Personality and city culture predict attitudes and practices toward mosquitoes and mosquito-borne diseases in South Texas
Authors: Bohmann Amy K. , Martinez-Berman Lisset , Senger Amy R. , Wise de Valdez Megan R.
Published: November 2022

Personality is known to affect compliance with health-protective behaviors and it has been shown that effective public health messaging can be informed by an understanding of that relationship. Thus, we aimed to evaluate the role personality might play in implementing personal protective measures (PPMs) that can prevent mosquito-borne diseases. This is the first mosquito-related knowledge, attitudes, and practices (KAP) study to incorporate a measure of personality using the Big Five: openness, conscientiousness, extraversion, agreeableness, and neuroticism. KAP studies in Gulf-coast and Mexican border-states in the U.S. are few. Ours is only the second KAP study to take place in Texas despite known local transmission and established mosquito populations capable of transmitting dengue, zika, chikungunya, and West Nile viruses. The KAP survey was administered in three neighborhoods in San Antonio, a large, Hispanic-majority, urban city that is segregated economically and ecologically. We found that openness, agreeableness, and extraversion predicted certain attitudes and PPMs, and that KAP and personality measures did not differ along ethnic or neighborhood lines. Perceptions toward the city's role in mosquito control and education was an important factor in predicting PPMs, suggesting that city culture (attitudes common throughout the city as opposed to attitudes differing by ethnicity and neighborhood) may be most salient in developing public health messaging in San Antonio.
Effectiveness and feasibility of self-sampling for human papillomavirus testing for internet-based cervical cancer screening
Authors: Li Jingran , Wu Ruifang , Qu Xinfeng , Huang Xia , Li Ling , Lin Zhixin , Zhang Zhijun , Deng Jihong , Liu Rong , Zhao Xiaofeng , Zhang Songling , Lin Bei , An Ruifang , Zhao Chao , Li Mingzhu , Zhao Yun , Wei Lihui
Published: November 2022

Objectives

Worldwide, around 18.2% of cervical cancer occurred in China, mainly because of lower screening coverage and screening quality in regional disparities. To assess self-sampling for human papillomavirus (HPV) testing, combined with the internet, as a primary cervical cancer screening (CCS) method in low-resource settings, and to establish an internet-based self-sampling CCS-management model.

Methods

The women who participated registered on a CCS website. We recruited 20,136 women, aged 30–59 years, from 13 provinces in China, to perform vaginal self-sampling for HPV testing as a primary CCS, based on the internet. A questionnaire was subsequently used to investigate the acceptability of self-sampling.

Results

Of the 20,103 women with qualified samples, 35.80% lived in remote areas, 37.69% had never undergone CCS, 59.96% were under-screened, and the overall prevalence of a high-risk of HPV was 13.86%. Of 8,136 respondents, 95.97% of women felt that self-sampling was easy to perform, 84.61% had no discomfort when using a self-sampling brush, 62.37% women were more likely to choose self-sampling for CCS in future, and 92.53% were willing to introduce the concept to others around them. The reliability and ease of self-sampling were independent factors influencing selection of self-sampling (p < 0.05).

Conclusions

The Internet-facilitated self-sampling for HPV testing and management model for cervical cancer prevention is feasible and effective. It can be used as a supplement to the conventional screening, particularly in outlying areas with few medical resources, to improve the coverage of CCS.

Clinical trial registration

https://www.chictr.org.cn, identifier: ChiCTR2000032331.