bluuebunny/crossref_metadata_2025 huggingface dataset:
Phosphorus fractions and their relationships with soil chemical attributes in an integrated crop-livestock system under annual phosphates fertilization
Authors: Guera Keli Cristina Silva , da Fonseca Adriel Ferreira
Published: November 2022

The main factors affecting phosphorus (P) availability in soils are mineralogy, acidity, and concentration of elements in the soil solution. Moreover, P fertilizer sources and amounts can affect P cycling and adsorption of this element on colloids. In this research, we hypothesized that the use of phosphate sources of different solubilities can alter soil P adsorption process, due to chemical compositions of these sources and, in ICLS, the soil-plant-animal system can change the P forms distribution on soil profile and its interactions with the soil chemical attributes. To examine these hypotheses, a field experiment was conducted over 5 years, under a Haplic Cambisol, in an incomplete factorial scheme, with the treatments being composed of three P sources (triple superphosphate, rock phosphate—Arad and magnesium thermophosphate), three doses of total P2O5 (60, 120, and 180 kg ha−1) plus a treatment without application of phosphate fertilizer. Phosphate applications occurred annually, broadcast without incorporation, at the time of sowing the annual winter forage, in a crop rotation system that included pasture in autumn-winter and grain crops in spring-summer. Soil samples were collected at depths of 0 to 5, 5 to 10, 10 to 15, 15 to 20, and 20 to 30 cm and evaluated by Hedley P fractionation and the soil chemical attributes were quantified. The use of different phosphates applied broadcast without incorporation did not influence the concentrations of soil P fractions over a 5 year study. Phosphate fertilizer doses above 120 kg ha−1 of total P2O5 increased moderately labile and non-labile P fractions. The highest concentration of labile P was found up to 15 cm soil depth. It was found that P lability is strongly associated with calcium and total organic carbon concentrations in the soil profile. High concentrations of basic cations and sulfate in the soil solution increased moderately labile and non-labile P fractions. The complexity of ICLS interactions to maintain phosphorus dynamics in the soil was shown to be an intricate P release/adsorption process associated with soil chemical attributes.
How accurate is the visual estimation of bowel length by endoscopic surgeons?
Authors: Mirzaee Sahar , Golzarand Mahdieh , Parsaei Reza , Toolabi Karamollah , Amirbeigi Alireza
Published: November 2022

Background

Measurement of small bowel length is an essential step in performing bariatric surgery. Surgeons need to measure bowel length in order to create alimentary and biliopancreatic limbs. Inaccurate bowel measurement may affect the outcome of surgery. However, it is not clear how accurate the measurement of bowel length is by surgeons.

Methods

Two image quizzes marking certain lengths of jejunum were sent to participants. They were asked to estimate the length of marked bowels in maze quizzes. The Error of estimation, prevalence of significant error (error greater than 30 percent of actual length), and the relationship between different participant characteristics was investigated.

Results

A total of 86 participants answered the questionnaire. The mean error of estimation was 4.62 cm (27%). Twenty-eight participants (33%) had significant errors in estimation of bowel length.

Conclusion

While there are surgeons that can estimate bowel length with decent accuracy, significant errors in estimation of bowel length are not uncommon among surgeons. Surgeons should consider adopting techniques for accurate measurement of the small intestine.
Biomechanical studies of different numbers and positions of cage implantation on minimally invasive transforaminal interbody fusion: A finite element analysis
Authors: Han Zhenchuan , Ma Chao , Li Bo , Ren Bowen , Liu Jianheng , Huang Yifei , Qiao Lin , Mao Keya
Published: November 2022

Background

The position and number of cages in minimally invasive transforaminal interbody fusion (MIS-TLIF) are mainly determined by surgeons based on their individual experience. Therefore, it is important to investigate the optimal number and position of cages in MIS-TLIF.

Methods

The lumbar model was created based on a 24-year-old volunteer's computed tomography data and then tested using three different cage implantation methods: single transverse cage implantation (model A), single oblique 45° cage implantation (model B), and double vertical cage implantation (model C). A preload of 500 N and a moment of 10 Nm were applied to the models to simulate lumbar motion, and the models' range of motion (ROM), ROM ratio, peak stress of the internal fixation system, and cage were assessed.

Results

The ROM ratios of models A, B, and C were significantly reduced by >71% compared with the intact model under all motions. Although there were subtle differences in the ROM ratio for models A, B, and C, the trends were similar. The peak stress of the internal fixation system appeared in model B of 136.05 MPa (right lateral bending), which was 2.07 times that of model A and 1.62 times that of model C under the same condition. Model C had the lowest cage stress, which was superior to that of the single-cage model.

Conclusion

In MIS-TLIF, single long-cage transversal implantation is a promising standard implantation method, and double short-cage implantation is recommended for patients with severe osteoporosis.
Case report: Surgical resection of a retro-hepatic leiomyosarcoma involving atrial reconstruction, cardiopulmonary bypass, ex vivo tumor resection, and liver re-implantation
Authors: Sharma Neel K. , Okakpu Uchenna , Murthy Jeevan , Wei Lawrence M. , Lopez-Solis Roberto , Schmidt Carl , Badhwar Vinay , Marsh J. Wallis
Published: November 2022

Introduction

Leiomyosarcomas (LMS) involving the inferior vena cava (IVC) is a clinically rare entity, accounting for approximately 0.5% of all adult sarcomas.

Case presentation

A 67-year-old male presented to the emergency department with mild back and lower abdominal pain. During the workup, a computed tomography scan without contrast showed an area of decreased attenuation within the liver adjacent to the intrahepatic IVC. Magnetic resonance imaging confirmed the involvement of the retro-hepatic IVC; biopsy confirmed the diagnosis of LMS. Given the location of the involvement of the retro-hepatic IVC, liver explantation was deemed necessary for adequate tumor resection. The superior extension of the tumor toward the heart necessitated Cardio-Pulmonary (CPB). The patient successfully underwent a complex surgical procedure involving liver explantation with ex vivo back-table resection of the retro-hepatic LMS, replacement of the retro-hepatic vena cava with a ringed Gore-Tex graft, liver re-implantation, and hepatic vein-atrial reconstruction under cardiopulmonary bypass. There were no intraoperative or post-op complications.

Discussion

The role of vascular reconstruction of the IVC varies depending on the level and extent of the tumor, with options ranging from primary repair, ligation, or reconstruction dictated. Surgical resection with negative margins remains the treatment of choice due to the lack of efficacy of adjuvant therapies. Importantly, liver explantation offers a chance for complete surgical resection and reconstruction. Similarly, the complex nature of the tumor necessitated a pioneering approach involving direct hepato-atrial venous anastomosis.

Conclusion

To the best of our knowledge, this is the first reported case in which the hepatic veins were anastomosed directly to the right atrium while also replacing the native vena cava with a separate graft.
Prognostic analysis of hepatocellular carcinoma with macrovascular invasion after liver resection and a successful case of conversion therapy
Authors: Ji Mengling , Zou Hao , Shu Baojun , Liu Guoping , Zhang Bingyuan , Xu Zejiang , Pang Fanfan , Cheng Mengxing , Sun Yafei , Du Ting , Sun Chuandong , Zhu Chengzhan
Published: November 2022

Objective

Macrovascular invasion (MVI) is an important factor leading to poor prognosis in hepatocellular carcinoma (HCC). Liver resection may offer favorable prognosis for selected patients with HCC. This study aimed to analyze the prognostic factors of HCC with MVI after liver resection as well as demonstrate a case of conversion therapy in an HCC patient with portal vein tumor thrombus (PVTT).

Methods

A total of 168 HCC patients with MVI who underwent primary liver resection at the Affiliated Hospital of Qingdao University between January 2013 and October 2021 were enrolled in the study. Clinicopathological data were collected retrospectively. Univariate and multivariate regression analyses were used to investigate the risk factors influencing recurrence and overall survival. Additionally, conversion therapy with drug-eluting bead transarterial chemoembolization (D-TACE), and sorafenib plus sintilimab treatment was performed in an HCC patient with PVTT.

Results

Among the 168 patients with HCC, 11 were diagnosed with hepatic vein tumor thrombosis, and the rest were diagnosed with PVTT. The 1-year disease-free survival rate was 37.5%, and the 3-year overall survival rate was 52.7%. Univariate and multivariate regression analyses revealed that HBsAg positivity, alpha-fetoprotein (AFP) level ≥400 ng/ml, liver capsule invasion, and tumor number ≥2 were independent prognostic factors for tumor recurrence, whereas HBsAg positivity was an independent risk factor for overall survival. Postoperative prophylactic medication did not significantly prolong the recurrence time. The median survival time (MST) after tumor recurrence was 13.4 months. In the patient treated with conversion therapy, the tumor gradually shrank and was eventually surgically resected.

Conclusions

This study identified the independent prognostic and risk factors associated with recurrence and overall survival in HCC patients with MVI. Additionally, we successfully performed conversion therapy in an HCC patient with PVTT. The findings would help identify patients at high risk of recurrence and indicate that combined therapy may prolong the survival of HCC patients with PVTT.
Cerebrovascular disorders associated with agenesis of the internal carotid artery: Findings on digital subtraction angiography
Authors: Xu Xiaolong , Shen Hongjian , Ma Hongyu , Zhang Xiaoxi , Zhang Lei , Li Qiang , Zhao Rui , Dai Dongwei , Li Zifu , Yang Pengfei , Liu Jianmin
Published: November 2022

Objective

Agenesis of the internal carotid artery (ICA) is a rare vascular condition that is complicated by intracranial aneurysms and rete mirabile. The altered hemodynamics caused by this distinctive cerebrovascular angioarchitecture can cause ischemic or hemorrhagic accidents. Data on clinical and radiographic features have been limited to describing this vascular pattern. We present five cases of agenesis of the internal carotid artery confirmed by digital subtraction angiography (DSA) and further investigate the influence of altered angioarchitecture on the integrity of intracranial morphology.

Methods

Cases of ICA anomalies were screened from the patients who underwent DSA in two hospitals. Clinical manifestation, radiographic features, management, and outcomes were retrospectively reviewed.

Results

Five patients [mean age 44 years (range, 30–65 years)] were included. Two patients presented with subarachnoid hemorrhage, one with cognitive impairment, one with dizziness, and one with intermittent headache. DSA demonstrated that three cases were complicated by intracranial aneurysms, one by dural arteriovenous fistula, and one by rete aneurysm. Three patients underwent endovascular treatment and one underwent bypass surgery. No patient died or experienced cerebrovascular accident during short-term follow-up.

Conclusions

ICA agenesis can be complicated by disorders such as intracranial aneurysm, rete aneurysm, and dural arteriovenous fistula. This suggests that ICA agenesis is associated with a tendency towards disrupted cerebrovascular homeostasis resulting from altered hemodynamics.
Antagonism of cadmium-induced liver injury in ducks by α-bisabolol
Authors: Elazab Sara T. , Hsu Walter H.
Published: November 2022

Cadmium (Cd) is an ecological pollutant which causes hazardous effects in animals and humans. The aim of this study was to investigate the role of α-bisabolol (BISA) in antagonizing the Cd-induced hepatotoxicity in ducks. Two-week old ducks were allocated into 8 groups (10 ducks/group): Group I received basal diet and was gavaged with sunflower oil (BISA vehicle, 1.1 mL/kg/day); group II was administered BISA orally (50 mg/kg/day; diluted with sunflower oil); groups III, IV, and V were fed the basal diet mixed with CdCl2at 37.5, 75, and 150 mg/kg diet, respectively, and were gavaged with sunflower oil; group VI, VII, and VIII were given basal diet containing CdCl2at the aforementioned consecutive doses plus BISA. All treatments were provided daily for 4 weeks. Exposure to CdCl2induced mortality in ducks, increased hepatic Cd content and serum levels of hepatopathic biomarkers, and caused oxidative stress and morphological alterations in ducks' liver. Furthermore, exposure to Cd caused upregulation of the mRNA of proinflammatory cytokine tumor necrosis factor-α and apoptotic gene Bax, and that of cyclooxygenase-2 protein in the liver. All effects of Cd were dose-dependent. BISA antagonized all of the aforementioned CdCl2-induced changes. These findings suggested that BISA exert the hepatoprotective effect against Cd toxicity through reducing the hepatic content of Cd as well as antagonizing oxidative insults, inflammation, and apoptosis. Thus, BISA has a great potential to be used as an antidote in the control of Cd poisoning.
Modified Sijunzi granule decreases post-weaning diarrhea in Rex rabbits via promoting intestinal development
Authors: Li Dongbo , Wang Yueli , Liu Ning , Chen Shiqi , Liu Hanzhong , Wang Ping , Yu Zhiju , Shu Gang , Lin Juchun , Zhang Wei , Peng Guangneng , Zhao Ling , Tang Huaqiao , Zhang Kai , Wen Bin , Fu Hualin
Published: November 2022

Traditional Chinese medicine (TCM) formulas can be adjusted on the basis of TCM basic theory to achieve the best curative effect, especially for diseases with complex pathogenesis, such as post-weaning diarrhea (PWD). Shugan Jianwei Sijunzi decoction (SJ-SJZD) can be recognized as modified Sijunzi Decoction (SJZD) supplemented with Astragalus mongholicus Bunge, Bupleurum chinense DC, Citrus × aurantium L., and Crataegus pinnatifida Bunge (fruit) in a fixed dosage ratio. The inactive ingredients were subsequently added to make granule, which was Shugan Jianwei Sijunzi granule (SJ-SJZG). Previous studies have confirmed the antagonism of SJ-SJZG to PWD. However, the mechanism of SJ-SJZG protective effects on small intestine in weaned Rex rabbits remained unclear. Animals were randomly divided into negative control (NC), low dose (LD), medium dose (MD), high dose (HD), and positive control (PC). SJ-SJZG significantly increased the intestinal length and the jejunum villi length. The SIgA level was statistically increased in duodenum and jejunum with the ELISA. Immunohistochemical detection showed that SIgA protein expression was also increased significantly in jejunum. Meanwhile, the relative expression of Zo1 in duodenum and jejunum of SJ-SJZG group increased significantly. SJ-SJZG significantly increased the relative expression of occludin in duodenum and jejunum as well. Moreover, real-time PCR results showed a significant increase in GLUT2 and SGLT1 relative expression in ileum. SJ-SJZG could also obviously enhance the expression of GLUT2 in jejunum and the expression of SGLT1 in duodenum. In conclusion, SJ-SJZG had been proven to be effective in promoting the development of small intestine and improving the immunity of small intestine. Moreover, SJ-SJZG could ensure the integrity of mucosal barrier and increase the ability of intestine to absorb glucose in small intestine.
Factors Contributing to Self-Medication and Consumption of Non-Prescribed Drugs in Portugal
Authors: Tavares Aida Isabel , Ferreira Pedro Lopes , Cavadas Veronica
Published: November 2022

Objectives: This work sets out to find the relationship between taking non-prescribed drugs and predisposing, enabling and need factors. Specifically, our main aim is to find the relationship between taking non-prescribed drugs and the lack of health care.

Methods: We used data from the last 2019 National Health Survey and estimate logistic regressions for the whole sample and stratified by sex.

Results: The most striking finding is that people self-medicating with non-prescribed drugs seem to be replacing health care when this is not used because of financial constraints or distance from provider. This suggests that non-prescribed drugs are a fast, affordable, alternative to health care. Other findings show that income and the financial resources to cope with unexpected expenditure are considerations in taking these drugs. Health and needs are other factors triggering their consumption.

Conclusion: Policy measures need to be aimed at improving access to medical care, providing responses to health needs such as those arising from chronic pain, and improving health literacy.
KIAA1199 Correlates With Tumor Microenvironment and Immune Infiltration in Lung Adenocarcinoma as a Potential Prognostic Biomarker
Authors: Shen Xiaoju , Mo Xiaocheng , Tan Weidan , Mo Xiaoxiang , Li Li , Yu Fei , He Jingchuan , Deng Zhihua , Xing Shangping , Chen Zhiquan , Yang Jie
Published: November 2022

Background: KIAA1199 has been considered a key regulator of carcinogenesis. However, the relationship between KIAA1199 and immune infiltrates, as well as its prognostic value in lung adenocarcinoma (LUAD) remains unclear.

Methods: The expression of KIAA1199 and its influence on tumor prognosis were analyzed using a series of databases, comprising TIMER, GEPIA, UALCAN, LCE, Prognoscan and Kaplan-Meier Plotter. Further, immunohistochemistry (IHC), western blot (WB) and receiver operating characteristic (ROC) curve analyses were performed to verify our findings. The cBioPortal was used to investigate the genomic alterations of KIAA1199. Prediction of candidate microRNA (miRNAs) and transcription factor (TF) targeting KIAA1199, as well as GO and KEGG analyses, were performed based on LinkedOmics. TIMER and TISIDB databases were used to explore the relationship between KIAA1199 and tumor immune infiltration.

Results: High expression of KIAA1199 was identified in LUAD and Lung squamous cell carcinoma (LUSC) patients. High expression of KIAA1199 indicated a worse prognosis in LUAD patients. The results of IHC and WB analyses showed that the expression level of KIAA1199 in tumor tissues was higher than that in adjacent tissues. GO and KEGG analyses indicated KIAA1199 was mainly involved in extracellular matrix (ECM)-receptor interaction and extracellular matrix structure constituent. KIAA1199 was positively correlated with infiltrating levels of CD4+ T cells, macrophages, neutrophil cells, dendritic cells, and showed positive relationship with immune marker subsets expression of a variety of immunosuppressive cells.

Conclusion: High expression of KIAA1199 predicts a poor prognosis of LUAD patients. KIAA1199 might exert its carcinogenic role in the tumor microenvironment via participating in the extracellular matrix formation and regulating the infiltration of immune cells in LUAD. The results indicate that KIAA1199 might be a novel biomarker for evaluating prognosis and immune cell infiltration in LUAD.
Model Predictive Control Based on ILQR for Tilt-Propulsion UAV
Authors: Xia Jiyu , Zhou Zhou
Published: November 2022

The transition flight of tilt-propulsion UAV is a complex and time-varying process, which leads to great challenges in the design of a stable and robust controller. This work presents a unified model predictive controller, which can handle the full envelope from vertical take-off and landing to cruise flight, to mean that the UAV can achieve a near-optimal transition flight under uncertainty conditions. Firstly, the nonlinear dynamic model of the tilt-propulsion UAV is developed, in which the aerodynamic/propulsion coupling effect of the ducted propeller is considered. Then, a control framework, including global trajectory planning and finite horizon control, is designed. Taking the planned global trajectory as the reference input, a controller is proposed with an inner layer based on ILQR optimization and an outer layer based on feedback correction and forward rolling of the MPC frame. The ILQR-MPC controller has high computational efficiency to deal with nonlinear problems, and has the ability to give full play to UAV’s control ability and suppress uncertainty. Finally, the simulation results show that ILQR-MPC controller obviously performs better than the ILQR feedforward controller, and gains a scheduling PID controller and MPC controller.
A Conceptual Design and Optimization Approach for Distributed Electric Propulsion eVTOL Aircraft Based on Ducted-Fan Wing Unit
Authors: Ma Tielin , Wang Xiangsheng , Qiao Nanxuan , Zhang Zilun , Fu Jingcheng , Bao Mingmin
Published: November 2022

The distributed electric propulsion (DEP) eVTOL aircraft has gained rising interest for its promising potential in high-speed cruise compared with conventional tilt-rotor configuration. The aerodynamic interference of the DEP units and wing could become more complicated with a variable thrust in multiple flight conditions. Thus, it requires considerable effort to trade off in the whole design process. Aimed at improving the design efficiency in iteration cycling of a ducted-fan DEP eVTOL aircraft, a conceptual design and optimization approach is proposed in this paper regarding the single-ducted fan and its surrounding wing section as the basic unit. The optimization of the ducted-fan wing (DFW) unit is targeted at improving both hover and cruise efficiencies. After the verification of the span independence of the lift-and-drag coefficients of the DFW unit, a novel DEP eVTOL aircraft conceptual design approach is established based on the vertical meridional plane DFW unit performance analysis. In the following case study, the optimized DFW unit and the conceptual method are applied on a canard configuration, achieving 720 km/h maximum speed, a hovering efficiency of 76.3%, and a 10.7 cruise lift-to-drag ratio. The remarkable performance and concise workflow in the case study both demonstrated the applicability and effectiveness of the proposed design schemes for DEP eVTOL aircraft.
A Resilient Forward-Looking Terrain Avoidance Warning Method for Helicopters
Authors: Chen Rui , Zhao Long
Published: November 2022

To reduce nuisance alarms caused by a fixed forward-looking boundary, this paper proposes a resilient forward-looking terrain avoidance method. The method constructed the resilient and adjustable forward-looking boundary model by using roll angle and navigation error, which makes the terrain data employed by the forward-looking alert closer to the terrain data overflown by the anticipated flight trajectory, reduces the nuisance alarm, and improves the reliability of the alert. Using real digital elevation maps and simulated flight testing, the proposed method was observed to provide alerting that is superior to the traditional forward-looking terrain avoidance technique.
Evaluating the Heterogeneous Impacts of Adoption of Climate-Smart Agricultural Technologies on Rural Households’ Welfare in Mali
Authors: Awotide Bola Amoke , Ogunniyi Adebayo , Olagunju Kehinde Oluseyi , Bello Lateef Olalekan , Coulibaly Amadou Youssouf , Wiredu Alexander Nimo , Kone Bourémo , Ahamadou Aly , Manyong Victor , Abdoulaye Tahirou
Published: November 2022

Climate change is negatively affecting agricultural production in the Sahel region. Climate-Smart Agricultural Technologies (CSATs) are disseminated to reduce these negative effects, and particularly those on resource-poor farm households. This article investigates the distributional impacts of the adoption of CSAT on-farm households’ welfare using a dataset that covers four regions, 32 communes, 320 villages, and 2240 households in Mali. Using an instrumental variable quantile treatment effects model, the paper addresses the potential endogeneity arising from the selection bias and the heterogeneity of the effect across the quantiles of the outcome variables’ distribution. The results show that the adoption of CSAT is positively associated with improved households’ welfare. The farmers’ decision to adopt any CSAT is influenced by access to credit, contact with extension agents, participation in training, access to information through the television, and being a member of any organization such as a cooperative society. Moreover, the effect of the adoption of CSAT on household welfare varies across the different households. In particular, the results show that the impact of the adoption of CSAT on households’ welfare is generally higher for the poorest (farmers located at the bottom tail of the distribution) end of the welfare distribution. The findings, therefore, highlight the pro-poor impact of the adoption of CSAT in the rural Malian context, as well as the need to tailor the CSAT interventions toward specific socio-economic segments of the rural population in Mali.
WG-3D: A Low-Cost Platform for High-Throughput Acquisition of 3D Information on Wheat Grain
Authors: Wu Wei , Zhao Yuanyuan , Wang Hui , Yang Tianle , Hu Yanan , Zhong Xiaochun , Liu Tao , Sun Chengming , Sun Tan , Liu Shengping
Published: November 2022

The three-dimensional (3D) morphological information of wheat grains is an important parameter for discriminating seed health, wheat yield, and wheat quality. High-throughput acquisition of 3D indicators of wheat grains is of great importance for wheat cultivation management, genetic breeding, and economic value. Currently, the 3D morphology of wheat grains still relies on manual investigation, which is subjective, inefficient, and poorly reproducible. The existing 3D acquisition equipment is complicated to operate and expensive, which cannot meet the requirements of high-throughput phenotype acquisition. In this paper, an automatic, economical, and efficient method for the 3D morphometry of wheat grain is proposed. A line laser binocular camera was used to obtain high-quality point-cloud data. A wheat grain 3D model was constructed by point-cloud segmentation, finding, clustering, projection, and reconstruction. Based on this, 3D morphological indicators of wheat grains were calculated. The results show that the root mean square error (RMSE) and mean absolute percentage error (MAPE) of the length were 0.2256 mm and 2.60%, the width, 0.2154 mm and 5.83%, the thickness, 0.2119 mm and 5.81%, and the volume, 1.7740 mm3 and 4.31%. The scanning time was around 12 s and the data processing time was around 3.18 s under a scanning speed of 25 mm/s. This method can achieve the high-throughput acquisition of the 3D information of wheat grains, and it provides a reference for in-depth study of the 3D morphological indicators of wheat and other grains.
Crab Apple (Malus spp.) Seed Tocopherol Profile: Impact of Genotype, Species, Purpose and Rootstock
Authors: Górnaś Paweł , Mišina Inga , Ikase Laila
Published: November 2022

Apples are one of the most popular crops in the world, grown for fresh consumption, processing, and ornamental purposes. In the present study, the seeds of thirty crab apple (Malus spp.) genotypes were analyzed to evaluate the tocopherol composition and find a crop-specific profile. The mean proportion (%) of tocopherol (T) homologues (α, β, γ, and δ) was as follows: α-T (45.8%), β-T (21.8%), γ-T (24.3%), δ-T (8.1%) with a mean content of 22.41, 10.89, 12.35, and 4.08 mg/100 g dry weight, respectively. The coefficient of variation was higher in γ-T (0.748), δ-T (0.648) and β-T (0.540), and about two times lower for α-T (0.320). The total content of tocopherols varied much less in studied genotypes (coefficient of variation 0.164). α-T was the predominant tocopherol homologue in twenty-four genotypes (33.4–79.0%), while γ-T (36.4–64.9%) was the predominant in the remaining six studied genotypes. Principal component analysis identified six groups based on the tocopherol profile. Variety, purpose (ornamental vs. edible), and species appear to be associated with tocopherol profile. Most Malus sp., M. × prunifolia, and edible genotypes were located in two groups characterized by twice the content of α-T over β-T, and similar content of both (α-T and β-T), respectively. In both cases the sum of α-T and β-T constituted about 80% of total tocopherols. Significant correlations among tocopherol homologues were obtained: positive between α-T vs. β-T and γ-T vs. δ-T, and negative between α-T vs. γ-T, α-T vs. δ-T, and γ-T vs. β-T. These can be explained by the biosynthetic pathway of those lipophilic bioactive compounds.
Can Intensified Pasture Systems Reduce Enteric Methane Emissions from Beef Cattle in the Atlantic Forest Biome?
Authors: Meo-Filho Paulo , Berndt Alexandre , Pezzopane José R. M. , Pedroso André F. , Bernardi Alberto C. C. , Rodrigues Paulo H. M. , Bueno Ives C. S. , Corte Rosana R. , Oliveira Patrícia P. A.
Published: November 2022

The objective of this study was to evaluate the effect of different pasture systems on beef steers’ performance, dry matter intake, enteric methane emission, carcass production, forage quality, and animal production per hectare (ha). The trial was conducted at Embrapa Southeast Livestock, São Carlos/SP, Brazil. Sixty Cachim beef steers (5/8 Charolais × 3/8 Zebu) with initial liveweights of 255 ± 7 kg were evaluated for two years under five different grazing production systems (EXT = Extensive; INT = Intensive; iCL = Integrated crop livestock; iCLF = Integrated crop livestock forest; iLF = Integrated livestock forest). The final liveweight was greater (p < 0.05) for the animals under the INT and iCL systems (484 ± 51 and 466 ± 79 kg, respectively) compared to animals in the iCLF, iLF and EXT systems (416 ± 57, 414 ± 50 and 429 ± 48 kg). The dry matter intake was significantly greater under the EXT system than it was under the iCL system (9.8 ± 2.1 and 7.5 ± 2.9 kg day−1). Regarding the emission intensity in relation to the liveweight gain per unit area (g CH4 kg LWG−1 ha−1 year−1), it differed significantly among the systems (EXT = 1.6; INT = 0.6; iCL = 0.8; ICLF = 1.1; ILF = 0.7). Similarly, the methane emission intensity differed in relation to the carcass production (kg CH4 kg−1 carcass; EXT = 0.496; INT = 0.250; iCL = 0.297; iCLF = 0.345; iLF = 0.286). Beef cattle that are raised in intensive and/or integrated pasture systems have a greater availability of forage mass and nutrients than those that are raised extensively. Pasture systems that undergo soil pH correction and fertilization, rotational grazing and/or integrated with maize cropping produce animals with greater average daily gain and final liveweights, thereby lessening the enteric methane emissions per kg of weight gain. In these systems, the efficiency in terms of the gain per land area is also greater, however, the systems that are integrated with a forest component (iLF and iCLF) are equal to that of the EXT system. The same pattern is observed in the intensity of the methane emission as for the efficiency of the animal gain per unit of land area.
Earthworm Lumbricus terrestris Contributes Nitrous Oxide Emission from Temperate Agricultural Soil Regardless of Applied Mineral Nitrogen Fertilizer Doses
Authors: Maslov Mikhail , Astaykina Angelika , Pozdnyakov Lev
Published: November 2022

Agriculture is the main contributor to nitrous oxide (N2O) emission, but the emission intensity can be controlled by various factors, in particular, the activity of earthworms, one of the most common groups of soil invertebrates. We conducted an incubation experiment to evaluate N2O emission in earthworm soil samples compared to non-earthworm ones with applications of high (200 kg ha−1) and low (50 kg ha−1) mineral N fertilizer doses. We assessed the cumulative N2O emission, the dynamics of the soil dissolved organic carbon, and the soil microbial carbon and nitrogen content, as well as the number of nirK and nirS gene copies in bulk soil samples and in isolates from the earthworms’ gut. Our study showed a significant role of the earthworm activity in changing the intensity of N2O emission after the application of mineral N fertilizers. The main factor leading to an increase in nitrous oxide emission in the presence of earthworms is the stimulation of free-living soil denitrifiers by the organic matter of the earthworms’ excretions, as well as the thorough mixing of plant residues and soil. Contrary to our expectations, earthworms did not increase the representation of nitrite reductase genes in soil, although the earthworm’s gut can be considered as a refugium for denitrifiers. Our results indicate a possible risk of increased N2O emission from arable temperate soils with an increase in earthworm populations as the climate warms, even if application rates of mineral fertilizers are reduced.
Control of Stored Agro-Commodity Pests Sitophilus granarius and Callosobruchus chinensis by Nitrogen Hypoxic Atmospheres: Laboratory and Field Validations
Authors: Aulicky Radek , Shah Jawad Ali , Kolar Vlastimil , Li Zhihong , Stejskal Vaclav
Published: November 2022

Given the complexity of the practical usage of controlled atmospheres for the protection of agro-commodities, several researchers have pointed out that there is not enough robust scientific documentation regarding the usage of inert gases for their widespread practical application. Therefore, this work evaluated various regimes of hypoxic and anoxic nitrogen atmospheres for the control of two key stored-product pests, in laboratory and under field conditions in silos. Sitophilus granarius and Callosobruchus chinensis were selected as the tested species since they are important pests of grain/rice or legumes in Europe and Asia. Under laboratory conditions, we tested nitrogen (N2) concentrations (from 95 to 100%) and exposure times (1–20 days) on the developmental stages of both pest species. In most developmental stages of S. granarius and C. chinensis, the shortest effective exposure was found for nitrogen concentration of 99%. Based on our laboratory tests, validation studies were subsequently carried out in semi-hermetic steel silos (25t) using continuous nitrogen saturation by on-site built swing pressure generators. It was found that a full control of all stages of S. granarius and C. chinensis was achieved in 11 days of nitrogen exposure, using concentrations ranging above 99% and below 100%. Our work shows that hypoxic nitrogen treatment can be effectively achieved in small steel silos under proper technological and environmental conditions.
Weed Management by In Situ Cover Crops and Anaerobic Soil Disinfestation in Plasticulture
Authors: Singh Gursewak , Ward Brian , Levi Amnon , Cutulle Matthew
Published: November 2022

Weeds negatively affect organic vegetable crop growth and profitability. Weed management is the greatest challenge for vegetable organic growers since control options are limited for organic vegetable production. Anaerobic soil disinfestation (ASD) is a novel non-chemical pest management technique that creates anoxic conditions in the topsoil layer for a limited time. ASD is primarily based on the addition of labile carbon sources to topsoil to promote anaerobic conditions driven by microorganisms in moist soil mulched with polyethylene film (polyfim). Field studies were conducted in the summer–fall of 2020 and 2021 to determine the efficacy of warm season cover crops used as carbon sources for ASD and their role in weed management. The study used a factorial experimental design with four cover crop residue treatments (sorghum-sudangrass, sunn hemp, both, or none) in two soil aeration conditions (aerated or non-aerated). Cover crops were grown for 75 days, incorporated into the soil, and sealed with totally impermeable film (TIF) clear mulch, followed by a 4-week ASD process. All incorporated cover crop treatments in non-aerated conditions generated moderate to higher anaerobic conditions (0–150 mV) and provided significantly higher (p < 0.05) weed control than all the other treatments tested or controls. Tomato plants transplanted in non-aerated, cover crops incorporated plots were more vigorous and produced higher yields than aerated plots. No phytotoxicity was observed on tomato plants following ASD treatment in any of the treatments tested. This study demonstrated that warm season cover crops could potentially serve as a carbon source for ASD in organic tomato production.
Compensatory Yield Responses of Young Native Warm-Season Grass Stands to Seasonal Changes in Harvest Frequencies
Authors: Temu Vitalis W. , Rutto Laban K. , Kering Maru K.
Published: November 2022

Defoliation management can significantly affect subsequent grassland’s forage productivity and sustainability. To assess the type and persistence of native warm-season grass (NWSG) yield responses to changes in defoliation intensity, a five-year harvest trial was conducted, in a randomized complete block design, at Virginia State University’s research farm. Yield responses of newly established indiangrass (Sorghastrum nutans L.). Nash, big bluestem (Andropogon gerardii Vitman), switchgrass (Panicum virgatum L.) and eastern gamagrass (Tripsacum dactyloides L.) stands to second-year changes in harvest regimes were monitored. In 2012, seedlings of these native grasses were transplanted in clean-tilled plots, with 16 plants in each pot. The field was not irrigated, but broadleaf weeds were manually controlled, and all plots were mowed in August and mid-November of 2013. Starting June 2014, each plot had three harvest-strips assigned to single, two, or three cuts per year from early June to mid-October using a plot-harvester; forage weights were recorded. Based on the recorded fresh and oven-dry sample weights, plot forage DM yields were estimated. Cumulative forage biomass of all three-cut strips flipped to single-cut increased by ≥30% and >50% for big bluestem. The second-year single-cut yields also outperformed those cut thrice since the first year by 22–51%. The second-year biomass increases from single-cut strips that were cut thrice in the first year demonstrated that flipping-triggered compensatory yield responses overshadowed the first- year losses in plant vigor. The compensatory yield increases continued to, but not beyond, the third year and varied between species. The yield responses also showed that magnitudes of defoliation management–triggered NWSG growth responses depend more on growing conditions during the recovery period than its actual duration.
Effect of Light Intensity on Gene Expression in Hypocotyl during the Elongation in a Leaf-Yellowing Mutant of Pepper (Capsicum annuum L.)
Authors: Mao Lianzhen , Dai Yunhua , Huang Yu , Sun Hao , Li Ziyu , Yang Bozhi , Zhang Zhuqing , Chen Wenchao , Ou Lijun , Liu Zhoubin , Yang Sha
Published: November 2022

Light is vital for plant growth and development, and the germination of many plant seeds and the development of seedlings are very sensitive to the light environment. Under no or low light conditions, pepper seedlings will accelerate the elongation of the hypocotyl to obtain light. To elucidate the molecular mechanism by which light regulates hypocotyl elongation in pepper, RNA sequencing was performed to analyze the hypocotyls and cotyledons of the yellowing mutant R24 under three different light intensity treatments. A total of 35,341 gene were identified; moreover, during the treatment, 9695 new genes and 13,123 differentially expressed genes (DEGs) were observed, respectively. Some genes related to brassino-lide receptor protein kinase BRI1, light capture proteins LHCA and LHCB, and auxin response factor may regulate the response of hot pepper cotyledons and hypocotyls to different light intensity. KEGG functional enrichment analysis revealed that the most abundant pathways were phenylpropane biosynthesis, plant hormone signal transduction, and carbon metabolism. This study provides a valuable reference for understanding the molecular mechanism of pepper’s response to different light intensities at the seedling stage and for improving the local light environment to overcome the hypocotyl elongation of pepper crop under low light conditions.
Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies
Authors: Krzysica Paulina , Verhoog Loes , de Vries Sonja , Smits Coen , Savelkoul Huub F. J. , Tijhaar Edwin
Published: November 2022

Cytokines like interferon (IFN)-γ, interleukin (IL)-2, IL-6, IL-10, and IL-12p40 are important biomarkers for characterizing the nature and strength of immune responses. It is important to be able to quantify the cytokines at the protein level in biological samples. Quantification of chicken cytokines is generally performed on the level of messenger RNA (mRNA) by quantitative polymerase chain reaction (qPCR) because very few capture ELISAs for the quantification of chicken cytokine proteins are commercially available. Here, we describe the optimization and validation of capture ELISAs for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-γ using commercially available antibodies and reagents. First, we determined the optimal concentrations of the antibodies. We then verified the ELISAs’ performance and established that the lower limit of detection (LLOD) for all cytokines was below 32 pg/mL. The ELISAs show the same binding characteristics for recombinant and native cytokines (parallelism was <15.2% CV). Values for inter-assay variation were consistently low and mostly <20% CV. Overall, the optimized capture ELISAs are sensitive (<32 pg/mL) and reliable tools to quantify chicken cytokines. These ELISAs can easily and inexpensively be utilized in any immunological lab and may therefore have wide applicability in immunological research for poultry.
Comparing Pears to Apples: Unlike Dogs, Cats Need Habituation before Lab Tests
Authors: Uccheddu Stefania , Miklósi Ádám , Gintner Sarolt , Gácsi Márta
Published: November 2022

Research on the socio-cognitive skills of different species often benefit from comparative experiments, however, the ecology of the species and development of the individuals may differently determine how they react to the same test situation. In this study, our aims were twofold: to observe and compare the spontaneous behaviour of companion cats and dogs in the same novel environment, and to habituate them (if needed) to the novel environment in the presence of their owners and an unfamiliar experimenter. The behaviour of 62 family cats, 31 experienced (which had the opportunity to meet strangers and explore unfamiliar places) and 31 inexperienced cats, and 27 family dogs was compared in an unfamiliar room. The subjects’ behaviour was coded during the first five minutes in the presence of two passive persons, their owners, and an unfamiliar experimenter. Then, based on a set of rules, first the owner, and then the experimenter tried to initiate interactions with the subjects and the subjects’ willingness to interact was evaluated. To pass the habituation test, subjects were expected to play with the experimenter or accept food from them. All dogs passed the test on the first occasion, while almost 60% of the cats failed. The cats’ experience did not play a significant role, in fact, younger cats passed more easily. We found marked differences between dogs and cats in all behavioural variables; compared to dogs, cats spent more time crouching and close to their box, while less time exploring or close to the owner. We did not find a difference in the cats’ behaviour based on their experience. Our results support the hypothesis that unlike dogs, cats need extensive habituation in a novel environment. This could partially be explained by the difference in the ecology and/or domestication process of the species, although developmental effects cannot be excluded. Our findings have fundamental consequences not only for the considerations of the testability of cats and on the interpretation of their data collected in the laboratory, but in a more general sense on developing comparative experiments.
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species
Authors: de Souza Marcelo Santos , Barcellos Suziane Alves , dos Santos Michelly da Silva , Gunski Ricardo José , Garnero Analía del Valle , de Oliveira Edivaldo Herculano Corrêa , O’Connor Rebecca E. , Griffin Darren K. , Kretschmer Rafael
Published: November 2022

Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cytogenetic analysis is still very scarce in this bird order. Thus, the aim of this study was to provide insight into the Charadriiformes karyotype, with emphasis on microchromosome evolution in three species of shorebirds—Calidris canutus, Jacana jacana, and Vanellus chilensis—combining classical and molecular approaches. Cross-species FISH mapping applied two BAC probes for each microchromosome, GGA10–28 (except GGA16). The experiments revealed different patterns of microchromosome organization in the species investigated. Hence, while in C. canutus, we found two microchromosomes involved in chromosome fusions, they were present as single pairs in V. chilensis. We also described a new chromosome number for C. canutus (2n = 92). Hence, this study contributed to the understanding of genome organization and evolution of three shorebird species.
Antimicrobial Resistance Profile of Common Foodborne Pathogens Recovered from Livestock and Poultry in Bangladesh
Authors: Rafiq Kazi , Islam Md Rafiqul , Siddiky Nure Alam , Samad Mohammed Abdus , Chowdhury Sharmin , Hossain K. M. Mozaffor , Rume Farzana Islam , Hossain Md Khaled , Mahbub-E-Elahi ATM , Ali Md Zulfekar , Rahman Moizur , Amin Mohammad Rohul , Masuduzzaman Md , Ahmed Sultan , Ara Rumi Nazmi , Hossain Muhammad Tofazzal
Published: November 2022

Multidrug-resistant (MDR) foodborne pathogens have created a great challenge to the supply and consumption of safe & healthy animal-source foods. The study was conducted to identify the common foodborne pathogens from animal-source foods & by-products with their antimicrobial drug susceptibility and resistance gene profile. The common foodborne pathogens Escherichia coli (E. coli), Salmonella, Streptococcus, Staphylococcus, and Campylobacter species were identified in livestock and poultry food products. The prevalence of foodborne pathogens was found higher in poultry food & by-product compared with livestock (p < 0.05). The antimicrobial drug susceptibility results revealed decreased susceptibility to penicillin, ampicillin, amoxicillin, levofloxacin, ciprofloxacin, tetracycline, neomycin, streptomycin, and sulfamethoxazole-trimethoprim whilst gentamicin was found comparatively more sensitive. Regardless of sources, the overall MDR pattern of E. coli, Salmonella, Staphylococcus, and Streptococcus were found to be 88.33%, 75%, 95%, and 100%, respectively. The genotypic resistance showed a prevalence of blaTEM, blaSHV, blaCMY, tetA, tetB, sul1, aadA1, aac(3)-IV, and ereA resistance genes. The phenotype and genotype resistance patterns of isolated pathogens from livestock and poultry had harmony and good concordance, and sul1 & tetA resistance genes had a higher prevalence. Good agricultural practices along with proper biosecurity may reduce the rampant use of antimicrobial drugs. In addition, proper handling, processing, storage, and transportation of foods may decline the spread of MDR foodborne pathogens in the food chain.