bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-Lasting Impairments in Quadriceps Mitochondrial Health, Muscle Size, and Phenotypic Composition Are Present After Non-invasive Anterior Cruciate Ligament Injury
Authors: Davi Steven M. , Ahn Ahram , White McKenzie S. , Butterfield Timothy A. , Kosmac Kate , Kwon Oh Sung , Lepley Lindsey K.
Published: January 2022

Introduction

Despite rigorous rehabilitation aimed at restoring muscle health, anterior cruciate ligament (ACL) injury is often hallmarked by significant long-term quadriceps muscle weakness. Derangements in mitochondrial function are a common feature of various atrophying conditions, yet it is unclear to what extent mitochondria are involved in the detrimental sequela of quadriceps dysfunction after ACL injury. Using a preclinical, non-invasive ACL injury rodent model, our objective was to explore the direct effect of an isolated ACL injury on mitochondrial function, muscle atrophy, and muscle phenotypic transitions.

Methods

A total of 40 male and female, Long Evans rats (16-week-old) were exposed to non-invasive ACL injury, while 8 additional rats served as controls. Rats were euthanized at 3, 7, 14, 28, and 56 days after ACL injury, and vastus lateralis muscles were extracted to measure the mitochondrial respiratory control ratio (RCR; state 3 respiration/state 4 respiration), mitochondrial reactive oxygen species (ROS) production, fiber cross sectional area (CSA), and fiber phenotyping. Alterations in mitochondrial function and ROS production were detected using two-way (sex:group) analyses of variance. To determine if mitochondrial characteristics were related to fiber atrophy, individual linear mixed effect models were run by sex.

Results

Mitochondria-derived ROS increased from days 7 to 56 after ACL injury (30–100%, P < 0.05), concomitant with a twofold reduction in RCR (P < 0.05). Post-injury, male rats displayed decreases in fiber CSA (days 7, 14, 56; P < 0.05), loss of IIa fibers (day 7; P < 0.05), and an increase in IIb fibers (day 7; P < 0.05), while females displayed no changes in CSA or phenotyping (P > 0.05). Males displayed a positive relationship between state 3 respiration and CSA at days 14 and 56 (P < 0.05), while females only displayed a similar trend at day 14 (P = 0.05).

Conclusion

Long-lasting impairments in quadriceps mitochondrial health are present after ACL injury and play a key role in the dysregulation of quadriceps muscle size and composition. Our preclinical data indicate that using mitoprotective therapies may be a potential therapeutic strategy to mitigate alterations in muscle size and characteristic after ACL injury.
Comparison of Single-Trait and Multi-Trait Genome-Wide Association Models and Inclusion of Correlated Traits in the Dissection of the Genetic Architecture of a Complex Trait in a Breeding Program
Authors: Merrick Lance F. , Burke Adrienne B. , Zhang Zhiwu , Carter Arron H.
Published: January 2022

Unknown genetic architecture makes it difficult to characterize the genetic basis of traits and associated molecular markers because of the complexity of small effect quantitative trait loci (QTLs), environmental effects, and difficulty in phenotyping. Seedling emergence of wheat (Triticum aestivum L.) from deep planting, has a poorly understood genetic architecture, is a vital factor affecting stand establishment and grain yield, and is historically correlated with coleoptile length. This study aimed to dissect the genetic architecture of seedling emergence while accounting for correlated traits using one multi-trait genome-wide association study (MT-GWAS) model and three single-trait GWAS (ST-GWAS) models. The ST-GWAS models included one single-locus model [mixed-linear model (MLM)] and two multi-locus models [fixed and random model circulating probability unification (FarmCPU) and Bayesian information and linkage-disequilibrium iteratively nested keyway (BLINK)]. We conducted GWAS using two populations. The first population consisted of 473 varieties from a diverse association mapping panel phenotyped from 2015 to 2019. The second population consisted of 279 breeding lines phenotyped in 2015 in Lind, WA, with 40,368 markers. We also compared the inclusion of coleoptile length and markers associated with reduced height as covariates in our ST-GWAS models. ST-GWAS found 107 significant markers across 19 chromosomes, while MT-GWAS found 82 significant markers across 14 chromosomes. The FarmCPU and BLINK models, including covariates, were able to identify many small effect markers while identifying large effect markers on chromosome 5A. By using multi-locus model breeding, programs can uncover the complex nature of traits to help identify candidate genes and the underlying architecture of a trait, such as seedling emergence.
A System Dynamics Approach to Model Photosynthesis at Leaf Level Under Fluctuating Light
Authors: Salvatori Nicole , Carteni Fabrizio , Giannino Francesco , Alberti Giorgio , Mazzoleni Stefano , Peressotti Alessandro
Published: January 2022

Photosynthesis has been mainly studied under steady-state conditions even though this assumption results inadequate for assessing the biochemical responses to rapid variations occurring in natural environments. The combination of mathematical models with available data may enhance the understanding of the dynamic responses of plants to fluctuating environments and can be used to make predictions on how photosynthesis would respond to non-steady-state conditions. In this study, we present a leaf level System Dynamics photosynthesis model based and validated on an experiment performed on two soybean varieties, namely, the wild type Eiko and the chlorophyll-deficient mutant MinnGold, grown in constant and fluctuating light conditions. This mutant is known to have similar steady-state photosynthesis compared to the green wild type, but it is found to have less biomass at harvest. It has been hypothesized that this might be due to an unoptimized response to non-steady-state conditions; therefore, this mutant seems appropriate to investigate dynamic photosynthesis. The model explained well the photosynthetic responses of these two varieties to fluctuating and constant light conditions and allowed to make relevant conclusions on the different dynamic responses of the two varieties. Deviations between data and model simulations are mostly evident in the non-photochemical quenching (NPQ) dynamics due to the oversimplified combination of PsbS- and zeaxanthin-dependent kinetics, failing in finely capturing the NPQ responses at different timescales. Nevertheless, due to its simplicity, the model can provide the basis of an upscaled dynamic model at a plant level.
Beneficial Effects of Exogenous Melatonin and Dopamine on Low Nitrate Stress in Malus hupehensis
Authors: Du Peihua , Yin Baoying , Cao Yang , Han Ruoxuan , Ji Jiahao , He Xiaolong , Liang Bowen , Xu Jizhong
Published: January 2022

Malus hupehensis, as an apple rootstock, is an economically important tree species popular due to its excellent fruit yield and stress resistance. Nitrogen is one of the critical limiting factors of plant growth and fruit yield, so it is crucial to explore new methods to improve nitrogen use efficiency. Melatonin and dopamine, as multifunctional metabolites, play numerous physiological roles in plants. We analyzed the effects of exogenous melatonin and dopamine treatments on the growth, root system architecture, nitrogen absorption, and metabolism of M. hupehensis when seedlings were exposed to nitrate-deficient conditions. Under low nitrate stress, plant growth slowed, and chlorophyll contents and 15NO3– accumulation decreased significantly. However, the application of 0.1 μmol/L melatonin or 100 μmol/L exogenous dopamine significantly reduced the inhibition attributable to low nitrate levels during the ensuing period of stress treatment, and the effect of dopamine was more obvious. In addition to modifying the root system architecture of nitrate-deficient plants, exogenous melatonin and dopamine also changed the uptake, transport, and distribution of 15NO3–. Furthermore, both exogenous melatonin and dopamine enhanced tolerance to low nitrate stress by maintaining the activity of enzymes (NR, NiR, GS, Fd-GOGAT, and NADH-GOGAT) and the transcription levels of related genes involved in leaf and root nitrogen metabolism. We also found that exogenous melatonin and dopamine promoted the expression of nitrate transporter genes (NRT1.1, NRT2.4, NRT2.5, and NRT2.7) in nitrate-deficient plant leaves and roots. Our results suggest that both exogenous melatonin and dopamine can mitigate low nitrate stress by changing the root system architecture, promoting the absorption of nitrate, and regulating the expression of genes related to nitrogen transport and metabolism. However, according to a comprehensive analysis of the results, exogenous dopamine plays a more significant role than melatonin in improving plant nitrogen use efficiency.
Differential Metabolomic Responses of Kentucky Bluegrass Cultivars to Low Nitrogen Stress
Authors: Sun Xiaoyang , Guo Zhixin , Jiang Yiwei , Qin Ligang , Shi Zhenjie , Dong Lili , Xiong Liangbing , Yuan Runli , Deng Wenjing , Wu Hanfu , Liu Qingqing , Xie Fuchun , Chen Yajun
Published: January 2022

Kentucky bluegrass (Poa pratensis L.) is a cool-season turfgrass species that responds strongly to nitrogen (N), but the metabolomic responses of this grass species to N supply is unknown. The N-tolerant cultivar Bluemoon and N-sensitive cultivar Balin were exposed to normal N (15 mM) and low N (0.5 mM) for 21 days for identification of differentially expressed metabolites (DEMs) between normal N and low N treatments. Balin had more reductions of chlorophyll and total soluble protein concentrations and a higher accumulation of superoxide radicals under low N stress. A total of 99 known DEMs were identified in either cultivar or both including 22 amino acids and derivatives, 16 carbohydrates, 29 organic acids, and 32 other metabolites. In Bluemoon, β-alanine metabolism was most enriched, followed by alanine, aspartate, and glutamate metabolism, biosynthesis of valine, leucine, and isoleucine biosynthesis, and glycine, serine, and threonine metabolism. In Balin, alanine, aspartate, and glutamate metabolism were most enriched, followed by the tricarboxylic acid (TCA), glyoxylate and decarbohydrate metabolism, and carbon fixation. Bluemoon generally maintained higher TCA cycle capacity and had more downregulated amino acids, while changes in more organic acids occurred in Balin under low N stress. Some metabolite changes by low-N stress were cultivar-specific. The results suggested that regulation of metabolites related to energy production or energy saving could contribute to low N tolerance in Kentucky bluegrass.
The Interprocessual-Self Theory in Support of Human Neuroscience Studies
Authors: Luis Elkin O. , Akrivou Kleio , Bermejo-Martins Elena , Scalzo Germán , Orón José Víctor
Published: January 2022

Rather than occurring abstractly (autonomously), ethical growth occurs in interpersonal relationships (IRs). It requires optimally functioning cognitive processes [attention, working memory (WM), episodic/autobiographical memory (AM), inhibition, flexibility, among others], emotional processes (physical contact, motivation, and empathy), processes surrounding ethical, intimacy, and identity issues, and other psychological processes (self-knowledge, integration, and the capacity for agency). Without intending to be reductionist, we believe that these aspects are essential for optimally engaging in IRs and for the personal constitution. While they are all integrated into our daily life, in research and academic work, it is hard to see how they are integrated. Thus, we need better theoretical frameworks for studying them. That study and integration thereof are undertaken differently depending on different views of what it means to live as a human being. We rely on neuroscientific data to support the chosen theory to offer knowledge to understand human beings and interpersonal relational growth. We should of course note that to describe what makes up the uniqueness of being, acting, and growing as a human person involves something much more profound which requires too, a methodology that opens the way for a theory of the person that responds to the concerns of philosophy and philosophical anthropology from many disciplines and methods (Orón Semper, 2015;Polo, 2015), but this is outside the scope of this study. With these in mind, this article aims to introduce a new explanatory framework, called the Interprocessual-self (IPS), for the neuroscientific findings that allow for a holistic consideration of the previously mentioned processes. Contributing to the knowledge of personal growth and avoiding a reductionist view, we first offer a general description of the research that supports the interrelation between personal virtue in IRs and relevant cognitive, emotional, and ethic-moral processes. This reveals how relationships allow people to relate ethically and grow as persons. We include conceptualizations and descriptions of their neural bases. Secondly, with the IPS model, we explore neuroscientific findings regarding self-knowledge, integration, and agency, all psychological processes that stimulate inner exploration of the self concerning the other. We find that these fundamental conditions can be understood from IPS theory. Finally, we explore situations that involve the integration of two levels, namely the interpersonal one and the social contexts of relationships.
Late Effects of Cancer Treatment, Job Resources, and Burnout Complaints Among Employees With a Breast Cancer Diagnosis 2–10 Years Ago: A Longitudinal Study
Authors: Boelhouwer Ingrid G. , Vermeer Willemijn , van Vuuren Tinka
Published: January 2022

Purpose

The aim of this study was to investigate the effect of possible late effects of cancer treatment (physical complaints, fatigue, and cognitive complaints) and of two job resources (autonomy and supportive leadership style) on future burnout complaints, among employees living 2–10 years beyond breast cancer diagnosis.

Methods

Data at T1 (baseline questionnaire) and at T2 (9 months later) were collected in 2018 and 2019 (N = 287). These data were part of a longitudinal study among Dutch speaking workers with a cancer diagnosis 2–10 years ago. All complaints and job resources were self-reported. Longitudinal multivariate regression analyses were executed, controlling for years since diagnosis, living with cancer (recurrence or metastasis), and other chronic or severe diseases. Mediation by baseline burnout complaints was considered.

Results

A higher level of fatigue and cognitive complaints at baseline (T1) resulted in higher future burnout complaints (at T2), with partial mediation by baseline burnout complaints. No effect of physical complaints at T1 was observed. Higher levels of autonomy or a supportive leadership style resulted in lower burnout complaints, with full mediation by baseline burnout complaints. Buffering was observed by autonomy in the relationship of cognitive complaints with future burnout complaints. No moderation was observed by supportive leadership.

Conclusion

The level of burnout complaints among employees 2–10 years beyond breast cancer diagnosis may be an effect of fatigue or cognitive complaints, and awareness of this effect is necessary. Interventions to stimulate supportive leadership and autonomy are advisable, the latter especially in the case of cognitive complaints.
Exploring the Nuanced Links Between Internet Use and Subjective Well-Being Among Older Adults: A Nordic Population-Based Study
Authors: Viklund Emilia W. E. , Forsman Anna K.
Published: January 2022

Aim

The aim was to explore the various associations between subjective well-being and internet use among older adults in two regions in Finland and Sweden.

Methods

The data was collected through a population-based survey (N = 9,386) as part of the GERDA project conducted in 2016. The connection between subjective well-being (measured by perceived meaningfulness, happiness and life satisfaction) and internet use (distinguishing between internet users, non-users and users with support, and diverse internet activities) was studied by conducting binary regression analyses, calculating odds ratios with 95% confidence intervals. The analyses also controlled for key subjective well-being covariates.

Results

Statistically significant associations were found between perceived life meaningfulness and internet use. When looking into the specific internet-based activities under study, activities related to leisure and entertainment showed statistically significant associations to perceived meaningfulness as well as perceived happiness, also after controlling for potential covariates. However, internet use and the different internet activities failed to show statistical significant associations to life satisfaction in the adjusted regression model.

Conclusion

The things we do on the internet (the activities) as well as how we conceptualize and measure subjective well-being in this type of research studies seem to matter when it comes to the relationship between subjective well-being and internet use in later life. Internet use and internet activities displayed various connections to the subjective well-being proxies used in this study. Therefore, the complexity and multidimensionality of both subjective well-being and internet use and related links need to be carefully explored in order to deepen our understanding of experienced well-being among older adults in a digitized world.
Psychometric Evaluation of the Parental Reflective Functioning Questionnaire in Chinese Parents
Authors: Ye Panqin , Ju Jiawen , Zheng Kejun , Dang Junhua , Bian Yufang
Published: January 2022

Parental reflective functioning (PRF) is important for parenting and child development. To effectively assess PRF in Chinese parents, this study aimed to revise the Parental Reflective Functioning Questionnaire (PRFQ) for the Chinese context. The original Chinese version of the PRFQ (PRFQ-C) was revised by following psychometric validation procedures in a sample of Chinese parents (N = 2,021, 1,034 mothers and 987 fathers). A series of psychometric analyses, including confirmatory factor analysis (CFA), internal consistency reliability analysis, discriminant validity, and criterion-related validity analysis, and analysis for measurement invariance between mothers and fathers, were conducted. The CFA results indicated that the final 12-item, three-factor model had a good fit {χ2(49)  =  472.381; CFI = 0.929; TLI = 0.904; RMSEA = 0.065, 90%CI = [0.060, 0.071]}. The Chinese version of the PRFQ with 12 items (PRFQ-12C) showed satisfactory reliability (omega = 0.68–0.82), discriminant validity [heterotrait-monotrait (HTMT) values < 0.85], and criterion-related validity. The PRFQ-12C also had measurement invariance across mothers and fathers. In conclusion, the PRFQ-12C is psychometrically sound and can be applied in China.
Impaired Verbal Memory Recall in Patients With Axonal Degeneration and Serum Glycine-Receptor Autoantibodies—Case Series
Authors: Hansen Niels , Bartels Claudia , Stöcker Winfried , Wiltfang Jens , Fitzner Dirk
Published: January 2022

Background

Glycine receptor antibody-associated neuropsychiatric disease is currently known to be dominated by the phenotypes stiff-person syndrome and progressive encephalomyelitis entailing rigidity and myoclonus. In our case series we aim to depict the less-often reported feature of cognitive impairment associated with glycine receptor antibodies.

Methods

We investigated five patients with cognitive impairment varying from mild cognitive impairment to dementia associated with serum glycine receptor antibodies. Mild and major neurocognitive disorders were diagnosed according to the DSM-5 (fifth edition of the Diagnostic and Statistical Manual of Mental Disorders). Neuropsychology via Consortium to Establish a Registry for Alzheimer's Disease (CERAD) testing results, psychopathology data via the Manual for the Assessment and Documentation of Psychopathology in Psychiatry (AMDP), cerebrospinal fluid analysis and magnetic resonance imaging data were retrospectively analyzed from patient files.

Results

We identified five patients with cognitive impairment as the main neuropsychiatric feature associated with serum glycine receptor antibodies. One patient also presented akinetic rigidity syndrome. The psychopathology comprised disorders of attention and memory, orientation, formal thought, and affect. In addition to suffering deficits in verbal memory function, figural recall, phonematic fluency, and globally deteriorated cognitive function, these patients presented seriously impaired memory recall in particular. Tau protein and phosphorylated tau protein 181 were elevated in 75% of patients.

Conclusions

Our results suggest that axonal neurodegeneration and especially impaired verbal memory recall in addition to deficits in verbal and figural memory characterize patients with progressive cognitive impairment associated with glycine receptor antibodies. This unresolved issue should be clarified by researchers to discover whether axonal degeneration is merely an age-related phenomenon or one related to glycine-receptor autoantibodies in old age. Cognitive impairment as a neuropsychiatric syndrome of glycine-receptor antibody disease is a potential, conceivable, but so far unproven additional feature requiring deeper large-scale investigations and consideration during differential diagnosis in memory clinics.
Mediating Effect of Body Mass Index and Dyslipidemia on the Relation of Uric Acid and Type 2 Diabetes: Results From China Health and Retirement Longitudinal Study
Authors: Cheng Fang , Li Yanzhi , Zheng Han , Tian Lu , Jia Hongying
Published: January 2022

Objective

This study assessed temporal relationships of serum uric acid (SUA) with blood glucose and determine the mediating effects of body mass index (BMI) and dyslipidemia on the relation of SUA and risk of type 2 diabetes.

Methods

Participants aged ≥ 45 years were participated in 2011 and followed up until 2015. Cox proportional hazards regression with a robust variance estimator was performed to explore the association of SUA with the risk of diabetes, and crosslagged path analysis was introduced to examine the temporal relationships between SUA and blood glucose. A mediation analysis was finally used to identify the mediating effect of BMI and dyslipidemia on the relation of SUA and the future risk of diabetes.

Results

A total of 9,020 participants were included with an average age of 58.59 years at baseline in 2011, and 53.6% of them were women. Linear dose–response relationship was identified by restricted spline cubic analysis between baseline SUA and follow-up blood glucose (the non-linear trend for fasting plasma glucose (FPG): β2 = −0.71, p = 0.52; for HbA1c: β2 = 0.05, p = 0.07; for risk of diabetes: β2 = 0.12, p = 0.39). Additionally, compared with the lowest quartiles of SUA, the adjusted risk ratios of diabetes were 1.00 (95% CI: 0.82–1.23), 1.08 (95% CI: 0.89–1.31), and 1.37 (95% CI: 1.11–1.96) for quartile 2–4 (p-trend < 0.01), respectively. Further additional adjustments for BMI or dyslipidemia, these ratios were not statistically significant. In addition, a unidirectional relationship from baseline SUA to follow-up FPG (ρ1 = 0.24, p = 0.03) was further confirmed using crosslagged path analysis. After stratifying by genders, the above results were only significant in the women subgroup, and we thus conducted a mediation analysis in women and found that the BMI and dyslipidemia partially mediated the effect of SUA on diabetes with a 23.05 and 18.82% mediating effect, respectively.

Conclusions

These findings provide strong evidence that hyperuricemia preceded diabetes, and the effect of baseline SUA on follow-up type 2 diabetes was more pronounced among middle-aged and elderly Chinese women, especially in postmenopausal women, and this effect is partly mediated by BMI and dyslipidemia at baseline.
Characteristics and Influencing Factors of Healthcare Utilization in Post-conflict Primary Care Attendees in Northern Sri Lanka
Authors: Doherty Shannon , Sureshkumar Diliniya Stanislaus , Thayakaran Rasiah , Surenthirakumaran Rajendra
Published: January 2022

Sri Lanka's healthcare systems attempts to provides access to universal healthcare services for all citizens and is designed to be free of out-of-pocket payments. Despite a 30-year civil conflict, natural disasters, and COVID-19, the healthcare system within the country remains robust and strong. However, due to a lack of formalized pathways and centralized record keeping, the pattern of service utilization is still relatively unknown, which raises concerns regarding effective allocation of scarce resources and efficiency of referral pathways. To address this gap in knowledge, part of the parent study (COMGAP-S), consisted of a survey on healthcare service use conducted among adults attending primary care facilities. The results from this quantitative data analysis indicate the majority of people seeking care originate from rural areas, are older (50+), attend divisional hospitals, and report paying fees at point of contact. Our findings indicate a need for more efficient use of healthcare services, creation of referral routes to ensure limited healthcare resources are used effectively. Additionally, further investment in services is needed to ensure Sri Lanka's healthcare system meets the standard of universal healthcare as proposed by the World Health Organization. These findings address a gap in knowledge for national decision-makers within Sri Lanka along with other similar post-conflict healthcare settings across the globe.
A Policy for Addressing Menstrual Equity in Schools: A Case Study From New York City, U.S.A.
Authors: Schmitt Margaret L. , Booth Kathleen , Sommer Marni
Published: January 2022

There has been a growth in menstrual equity policy advancements in the U.S.A. in recent years; with much of the new legislation prioritizing the needs of adolescent girls in schools. New York City, a predecessor of this movement, was the first U.S.A. locality to pass such legislation in 2016. The aim of this case study was to better understand the various factors which led to the development, passage and initial implementation of New York City's Menstrual Equity in Schools Policy. Data collection methods included a desk review and qualitative assessment with several actors involved across the policy and introduction phases. Key findings included (1) the utility of community narratives and a pilot project as a means for overcoming initial skepticisms, proving feasibility, and generating support; (2) the importance of policy champions for overcoming fiscal objections and navigating political discourse; and (3) lessons learned from early implementation efforts, including variance in awareness and distribution models. This case study yields valuable insights into the practical considerations when designing or implementing policies aimed at tackling issues of menstrual equity within school settings.
The N6-Methyladenosine Modification and Its Role in mRNA Metabolism and Gastrointestinal Tract Disease
Authors: Cai Teng , Atteh Lawrence Lawer , Zhang Xianzhuo , Huang Chongfei , Bai Mingzhen , Ma Haidong , Zhang Chao , Fu Wenkang , Gao Long , Lin Yanyan , Meng Wenbo
Published: January 2022

The N6-methyladenosine (m6A) modification is the most abundant internal modification of messenger RNA (mRNA) in higher eukaryotes. Under the actions of methyltransferase, demethylase and methyl-binding protein, m6A resulting from RNA methylation becomes dynamic and reversible, similar to that from DNA methylation, and this effect allows the generated mRNA to participate in metabolism processes, such as splicing, transport, translation, and degradation. The most common tumors are those found in the gastrointestinal tract, and research on these tumors has flourished since the discovery of m6A. Overall, further analysis of the mechanism of m6A and its role in tumors may contribute to new ideas for the treatment of tumors. m6A also plays an important role in non-tumor diseases of the gastrointestinal tract. This manuscript reviews the current knowledge of m6A-related proteins, mRNA metabolism and their application in gastrointestinal tract disease.
MALDI-TOF Mass Spectrometry as a Rapid Screening Alternative for Non-tuberculous Mycobacterial Species Identification in the Veterinary Laboratory
Authors: Lorente-Leal Víctor , Liandris Emmanouil , Bezos Javier , Pérez-Sancho Marta , Romero Beatriz , Juan Lucía de
Published: January 2022

Non-tuberculous mycobacteria (NTM) are difficult to identify by biochemical and genetic methods due to their microbiological properties and complex taxonomy. The development of more efficient and rapid methods for species identification in the veterinary microbiological laboratory is, therefore, of great importance. Although MALDI-TOF Mass Spectrometry (MS) has become a promising tool for the identification of NTM species in human clinical practise, information regarding its performance on veterinary isolates is scarce. This study assesses the capacity of MALDI-TOF MS to identify NTM isolates (n = 75) obtained from different animal species. MALDI-TOF MS identified 76.0% (n = 57) and 4% (n = 3) of the isolates with high and low confidence, respectively, in agreement with the identification achieved by Sanger sequencing of housekeeping genes (16S rRNA, hsp65, and rpoB). Thirteen isolates (17.3%) were identified by Sanger sequencing to the complex level, indicating that these may belong to uncharacterised species. MALDI-TOF MS approximated low confidence identifications toward closely related mycobacterial groups, such as the M. avium or M. terrae complexes. Two isolates were misidentified due to a high similarity between species or due to the lack of spectra in the database. Our results suggest that MALDI-TOF MS can be used as an effective alternative for rapid screening of mycobacterial isolates in the veterinary laboratory and potentially for the detection of new NTM species. In turn, Sanger sequencing could be implemented as an additional method to improve identifications in species for which MALDI-TOF MS identification is limited or for further characterisation of NTM species.
Molybdenum and Cadmium Co-induce Mitochondrial Quality Control Disorder via FUNDC1-Mediated Mitophagy in Sheep Kidney
Authors: Wu Yunhui , Yang Fan , Zhou Guangbin , Wang Qi , Xing Chenghong , Bai He , Yi Xin , Xiong Zhiwei , Yang Shuqiu , Cao Huabin
Published: January 2022

Molybdenum (Mo), fundamental trace mineral for animals and plants, but undue Mo damages animal health. Cadmium (Cd) is a toxic heavy metal that exists in the environment. Nevertheless, the mechanism of Mo and Cd on mitochondrial quality control are still indistinct. The objective of this research was to explore the effects of mitophagy on mitochondrial quality control via the FUNDC1-mediated by Mo and Cd in sheep kidney. Forty-eight 2-month-old sheep were stochastically divided into four groups, as shown below: control group, Mo [45 mg/kg body weight (BW)] group, Cd (1 mg/kg BW) group and Mo (45 mg/kg BW)+Cd (1 mg/kg BW) group, with 50 days feed technique. The results showed that Mo or/and Cd attract an unbalance of trace minerals and vacuoles and granular degeneration of renal tubular epithelial cells, and increase the number of mitophagosomes and vacuole-mitochondria and LC3 puncta and MDA and H2O2 contents, and decrease ATP content in the kidney. Moreover, Mo or/and Cd treatment could upregulate the mRNA levels of FUNDC1, LC3A, LC3B, PGAM5, DRP1, FIS1 and MFF, and the protein levels of FUNDC1, p-FUNDC1, LC3II/LC3I, DRP1, MFF and FIS1, downregulate the mRNA levels of MFN1, MFN2, OPA1, PGC-1α, SIRT1, SIRT3, FOXO1 and FOXO3, and the protein levels of MFN1, MFN2, OPA1 and PGC-1α. Notably, variations of above-mentioned factors in Mo and Cd group were more obvious than in Mo or Cd groups. Taken together, these results displayed that Mo and Cd co-treatment might induce mitochondrial quality control disorder via FUNDC1-mediated mitophagy in sheep kidney.
The Determinants of Length of Homeless Shelter Stays: Evidence-Based Regression Analyses
Authors: Hao Haijing , Garfield Monica , Purao Sandeep
Published: January 2022

Objective: To identify determinants that contribute to the length of homeless shelter stay.

Methods: We utilized a unique dataset from the Homeless Management Information Systems from Boston, Massachusetts, United States, which contains 44,197 shelter stays for 17,070 adults between Jan. 2014 and May 2018.

Results: Our statistical analyses and regression model analyses show that factors that contribute to the length of a homeless shelter stay include being female, senior, disability, being Hispanic, or being Asian or Black African. A significant fraction of homeless shelter stays (76%) are experienced by individuals with at least one of three disabilities: physical disability, mental health issues, or substance use disorder. Recidivism also contributes to longer homeless shelter stays.

Conclusion: The results suggest possible program and policy implications. Several factors that contribute to longer homeless shelter stay, such as gender, age, disability, race, and ethnicity, may have funding implications. Age may point to the need for early interventions. Disability is developmental and may benefit from treatment and intervention. Finally, we find that length of stay and recidivism are not independent, and may form a vicious cycle that requires additional investigation.
Applying Simheuristics to Minimize Overall Costs of an MRP Planned Production System
Authors: Seiringer Wolfgang , Castaneda Juliana , Altendorfer Klaus , Panadero Javier , Juan Angel A.
Published: January 2022

Looking at current enterprise resource planning systems shows that material requirements planning (MRP) is one of the main production planning approaches implemented there. The MRP planning parameters lot size, safety stock, and planned lead time, have to be identified for each MRP planned material. With increasing production system complexity, more planning parameters have to be defined. Simulation-based optimization is known as a valuable tool for optimizing these MRP planning parameters for the underlying production system. In this article, a fast and easy-to-apply simheuristic was developed with the objective to minimize overall costs. The simheuristic sets the planning parameters lot size, safety stock, and planned lead time for the simulated stochastic production systems. The developed simheuristic applies aspects of simulation annealing (SA) for an efficient metaheuristic-based solution parameter sampling. Additionally, an intelligent simulation budget management (SBM) concept is introduced, which skips replications of not promising iterations. A comprehensive simulation study for a multi-item and multi-staged production system structure is conducted to evaluate its performance. Different simheuristic combinations and parameters are tested, with the result that the combination of SA and SBM led to the lowest overall costs. The contributions of this article are an easy implementable simheuristic for MRP parameter optimization and a promising concept to intelligently manage simulation budget.
Recent Advances in Positive-Instance Driven Graph Searching
Authors: Bannach Max , Berndt Sebastian
Published: January 2022

Research on the similarity of a graph to being a tree—called the treewidth of the graph—has seen an enormous rise within the last decade, but a practically fast algorithm for this task has been discovered only recently by Tamaki (ESA 2017). It is based on dynamic programming and makes use of the fact that the number of positive subinstances is typically substantially smaller than the number of all subinstances. Algorithms producing only such subinstances are called positive-instance driven (PID). The parameter treedepth has a similar story. It was popularized through the graph sparsity project and is theoretically well understood—but the first practical algorithm was discovered only recently by Trimble (IPEC 2020) and is based on the same paradigm. We give an alternative and unifying view on such algorithms from the perspective of the corresponding configuration graphs in certain two-player games. This results in a single algorithm that can compute a wide range of important graph parameters such as treewidth, pathwidth, and treedepth. We complement this algorithm with a novel randomized data structure that accelerates the enumeration of subproblems in positive-instance driven algorithms.
A Numerical Study on Aircraft Noise Mitigation Using Porous Stator Concepts
Authors: Teruna Christopher , Rego Leandro , Casalino Damiano , Ragni Daniele , Avallone Francesco
Published: January 2022

This manuscript presents the application of a recently developed noise reduction technology, constituted by poro-serrated stator blades on a full-scale aircraft model, in order to reduce rotor-stator interaction noise in the fan stage. This study was carried out using the commercial lattice Boltzmann solver 3DS-SIMULIA PowerFLOW. The simulation combines the airframe of the NASA High-Lift Common Research Model with an upscaled fan stage of the source diagnostic test rig. The poro-serrations on the stator blades have been modeled based on a metal foam with two different porosity values. The results evidence that the poro-serrations induce flow separation on the stator blades, particularly near the fan-stage hub. Consequently, the thrust generated by the modified fan stage is lower and the broadband noise emission at low frequencies is enhanced. Nevertheless, the tonal noise components at the blade-passage frequency and its harmonics are mitigated by up to 9 dB. The poro-serrations with lower porosity achieve a better trade-off between noise emission and thrust penalty. An optimization attempt was carried out by limiting the application of porosity near the tip of the stator blades. The improved leading-edge treatment achieves a total of 1.5 dB in sound power level reduction while the thrust penalty is below 1.5%. This demonstrates that the aerodynamic effects of a leading-edge treatment should be taken into account during the design phase to fully benefit from its noise reduction capability.
Sequential Action of Different Fiber-Degrading Enzymes Enhances the Degradation of Corn Stover
Authors: Zhao Shengguo , Diaby Mohamed , Zheng Nan , Wang Jiaqi
Published: January 2022

Corn stover is one of the most agricultural residue abundances over the world; however, it is extremely prevented from microbial and enzymatic degradation into monomers because of the complex chemical and physical structure. In order to degrade corn stover, a large variety of enzymes with different specificities are required. However, each enzyme has its own reaction condition such as optimal pH and temperature to express its maximum activity. We hypothesize that the best sequence of an enzyme reaction could influence the degradation of corn stover. Therefore, the objective of this study was to investigate the effect of enzyme sequence action on the degradation of corn stover. A complete randomized design was used for this study. Four enzymes were used, cellulase (Cel) (pH 4.8 at 50 °C), hemicellulase (Hem) (pH 5 at 50 °C), pectinase (Pec) (pH 4 at 50 °C) and laccase (Lac) (pH 3 at 30 °C). This was subsequently submitted to enzyme sequence digestion following four steps (6 h incubation for each step) during which a single enzyme in each step was evaluated. The substrate (raw corn stover) was placed in sodium acetate buffer with an enzyme. The supernatant was then collected in each step for further chemical analysis. The results showed that there was a significant difference at p < 0.05 between treatments, suggesting that sequential action of fiber-degrading enzymes affected the chemical composition of corn stover. The best enzyme sequence (in terms of the total reducing sugar in different steps) was Hem-Cel-Pec-Lac (2.2 mg/mL) at p < 0.05; however, the worst enzyme sequence was Lac-Pec-Hem-Cel (0.8 mg/mL) at p < 0.05. Almost all the first steps in the process showed an increasing level of reducing sugar except the step which started with Lac where a lower reducing sugar level was observed. Similarly, xylose showed a higher level in all the processes in the first steps regardless of the enzyme type. It was observed that glucose production was totally dependent on the position of Cel in the enzyme sequence. Therefore, enzyme sequence action may be a useful method for corn stover to improve its degradation as feed stock.
Effects of NAA and Ecklonia maxima Extracts on Lettuce and Tomato Transplant Production
Authors: Moncada Alessandra , Vetrano Filippo , Esposito Alessandro , Miceli Alessandro
Published: January 2022

Ecklonia maxima and the commercial biostimulants produced from it contain various plant growth regulators that are responsible for the growth stimulation recorded in many crops. Auxins are one of the major plant growth regulators contained in E. maxima extracts. The aim of this research was to evaluate the growth-promoting effect of a seaweed extract from E. maxima on lettuce and tomato transplant production under nursery conditions, and to compare the effect of this extract with an equal concentration of synthetic auxin. Two doses of natural or synthetic exogenous auxins (50 or 100 μg L−1) were supplied to the substrate through the irrigation water with an ebb and flow system, 4, 11, and 18 days after sowing. A commercial biostimulant based on E. maxima extract was used as a source of natural auxin, while 1-naphthaleneacetic acid (NAA) was used as a synthetic auxin. Seedlings supplied only with water were used as a control. Tomato seedlings treated with 100 μg L−1 of natural auxins from E. maxima extract produced the tallest plants (+22%), with a higher leaf number (+12%), a wider leaf area (+44%), and a stronger stem (+12%), whereas lettuce seedling growth was promoted by all the treatments, but with a greater effect with increasing auxin supplementation and when using E. maxima extract, compared to NAA. The results showed that the supplementation of exogenous synthetic auxin (NAA), or an E. maxima extract containing natural auxins, can have a growth-promoting effect on lettuce and tomato seedlings. This effect was more evident on lettuce than tomato. The biostimulant produced from E. maxima extracts improved seedling quality and promoted shoot and root growth more than the NAA used as a synthetic source of auxins.
Zero-Tillage Effects on Durum Wheat Productivity and Soil-Related Variables in Future Climate Scenarios: A Modeling Analysis
Authors: Puig-Sirera Àngela , Acutis Marco , Bancheri Marialaura , Bonfante Antonello , Botta Marco , De Mascellis Roberto , Orefice Nadia , Perego Alessia , Russo Mario , Tedeschi Anna , Troccoli Antonio , Basile Angelo
Published: January 2022

Adoption of zero-tillage practices with residue retention in field crops has been introduced as an alternative soil-management technique to counteract the resource degradation and high production costs derived from intensive tillage. In this sense, the biophysical models are valuable tools to evaluate and design the most suitable soil-management technique in view of future climate variability. The aim of this study was to use the ARMOSA process-based crop model to perform an assessment of tillage (T) and no-tillage (No-T) practices of durum-wheat-cropping systems in the Campania region (South of Italy) under current and future climate scenarios. First, the model was calibrated using measurements of soil water content at different depths, leaf area index, and aboveground biomass in the T and No-T treatments during the 2013–2014 season. Then, the model was further applied in the T and No-T treatments to future climate data for 2020–2100 that was generated by the COSMO-CLM model using the RCP4.5 and 8.5 paths. Results of the calibration depicted that the model can accurately simulate the soil-crop-related variables of both soil-management treatments, and thus can be applied to identify the most appropriate conservation agricultural practices in the durum-wheat system. The simulation of soil water content at different depths resulted in small relative root mean square errors (RRMSE < 15%) and an acceptable Pearson’s correlation coefficient (r > 0.51); and the goodness-of-fit indicators for simulated LAI and AGB resulted in acceptable RRMSE (RRMSE < 28%), and high r (r > 0.84) in both soil-management treatments. Future climate simulations showed that No-T management will deliver 10% more wheat yield than the T, with an annual average 0.31% year−1 increase of soil organic carbon, and an increase of 3.80% year−1 for N uptake, which can diminish the N leaching. These results suggest that No-T could be implemented as a more resilient management for farming system in view of climate uncertainty and scarcity of resources. Therefore, these findings support the potential of the ARMOSA model to evaluate the soil-crop response of the durum-wheat system under different management conditions and to design appropriate soil-management practices for current and future climate predictions.
The Effect of Intruder Density on Territoriality and Dominance in Male Swimming Crab (Portunus trituberculatus)
Authors: Zhu Boshan , Zhang Hanzun , Lu Yunliang , Wang Fang , Liu Dapeng
Published: January 2022

Territorial behavior of animals is affected by numerous factors, one being the number of intruders. The swimming crab (Portunus trituberculatus), an important commercial and ecological species on the continental shelf of Asia, usually needs to defend its territory from intrusion by other crabs, especially in habitats with high densities of conspecifics. To clarify the underlying patterns of how P. trituberculatus protects its territory, we assessed the territorial behavior of occupant crabs (territory holders) when presented with different numbers of intruders using an indoor observation system. We calculated the territory size of the occupants and quantified their behavioral responses to intruders. With an increased number of intruders, the territory size the occupants owned significantly decreased, and their behavior adjusted accordingly. Besides, the territorial behavior score, reflecting the territoriality of crab, decreased significantly. Furthermore, in a high density group that had seven intruders, the occupants showed a higher dominance hierarchy than the intruders, indicating the ascendancy of occupants in territorial competition with intruders. These results revealed that as the number of intruders increased, the territory size of P. trituberculatus shrunk because the fight for territory became more intense.