bluuebunny/crossref_metadata_2025 huggingface dataset:
Bem-vindos ao cemitério Vera Cruz de Passo Fundo, RS
Authors: Farofa Renato Rodrigues
Published: July 2020

O título do livro organizado pelos historiadores Fernando Miranda e Gizele Zanotto bem poderia abordar um tema de ordem soteriológica, como o do cemitério, pelo víeis do campo das religiões. No entanto, a coletânea de textos do livro A morte não é o fim. Culturas e identidades no cemitério Vera Cruz leva o leitor a adentrar esse espaço cemiterial, localizado na cidade de Passo Fundo, no Rio Grande do Sul. Sua leitura nos guia à reflexão sobre possibilidades de pesquisa na própria necrópole, bem como em muitas outras no Brasil.
O escotismo e a morte em Portugal. A construção do imaginário “Eterno Acampamento”
Authors: Fontes Paulo Oliveira , Graça Gonçalo Brito
Published: July 2020

O presente artigo aborda a relação do universo escotista português com os conceitos de “morte” e “Eterno Acampamento” nas décadas de 1910 a 1930. As premissas do fundador inglês Robert Baden-Powell para que os jovens pudessem aspirar a uma cidadania ativa e produtiva, através de acampamentos e práticas pedagógicas em contextos orográficos agrestes, cedo provocaram divergências internas sobre a finalidade da formação cívica, social, moral e religiosa dos escoteiros, e estenderam-se aos cinco continentes. Porém, o caso português apresenta ainda um outro debate - o problema do destino metafísico dos escoteiros – que, depois de morrerem, para onde iriam as suas almas? Através da leitura da imprensa associativa portuguesa, pode observar-se que o “Eterno Acampamento” é muito mais que um lugar comum. A sua construção e utilização na gíria escotista conseguiu ser um ponto de convergência nos conflitos de interpretação religiosa sobre o destino final de todos estes jovens cidadãos.
A morte da criança hospitalizada: estratégias defensivas e de enfrentamento da equipe de enfermagem
Authors: Camponogara Silviamar , Miorin Jeanini Dalcol , Dias Gisele Loise , Rodrigues Isis de Lima , Vasconcelos Luísa Schirmann , Pinheiro Ana Lúcia Uberti
Published: July 2020

Este artigo busca identificar estratégias defensivas e de enfrentamento utilizadas pela equipe de enfermagem frente ao processo de morte da criança hospitalizada. É um estudo descritivo-exploratório de abordagem qualitativa. A coleta de dados deu-se através de entrevista semiestruturada com profissionais da equipe de enfermagem de unidade de Internação Pediátrica de hospital universitário no Sul do Brasil. As estratégias defensivas utilizadas para reduzir o sofrimento foram: não se envolver, mudança de foco, tentativa de separação do trabalho de atividades pessoais e diálogo da equipe. A estratégia de enfrentamento foi o apoio espiritual. Conclui-se que os trabalhadores necessitam de apoio psicológico para enfrentarem situações adversas presentes no trabalho. Com isso, ocorre o enfrentamento de situações de sofrimento e redução de efeitos desestabilizadoras no ambiente de trabalho.
Um lugar para morrer: experiências de morte domiciliar
Authors: Gomes Edlaine
Published: July 2020

Resenha do livro: Azevedo, Daniel Lima. O melhor lugar para morrer. Curitiba: Appris, 2020
A morte e a religiosidade no imaginário popular: o cemitério como local de culto e devoções marginais
Authors: Santana Julio Cesar Aguiar
Published: July 2020

Esta resenha tem como propósito, apresentar o livro "Milagreiros um estudo sobre três santos populares no Ceará (1929-1978)" de autoria de Michelle Ferreira Maia que trata das devoções marginais presentes no cotidiano da população cearense.
Apresentação do Dossiê 9: Doença e Morte
Authors: Menezes Rachel Aisengart , Luxardo Natalia
Published: July 2020

Redigimos esta apresentação em plena pandemia do covid-19. Ao lançarmos a chamada para o dossiê 9 da Revista M., com o tema “Doença e Morte”, não havia sombra ou sinal, tanto na imaginação e na fantasia quanto em dados observáveis ou observados e divulgados na mídia, do que viria a configurar a mais mortal das epidemias mundiais. Na chamada, afirmamos que em diferentes tempos, culturas, grupos e sociedades elaboraram – e elaboram – sistemas de classificação, leitura, entendimento e intervenção referentes ao que há algum tempo se convencionou chamar de saúde e, em oposição, doença. Com o processo de secularização iniciado há cerca de três séculos no Ocidente, à Medicina, baseada em saberes científicos, aos seus profissionais e instituições foram delegados os cuidados de doentes e, em especial, daqueles com enfermidades sem possibilidade de controle e/ou cura. Em suma, o processo do morrer passou a ser objeto de atenção e assistência de equipes de saúde, sobretudo a partir da segunda metade do século XX. A ênfase dos trabalhos apresentados nesse dossiê incide sobre a gestão da morte na instituição hospitalar, com artigos de autores procedentes de distintos países e regiões da América Latina.
“Lições sobre a Morte”: o discurso médico na obra de Pedro Nava
Authors: Gabriel Maria Alice Ribeiro
Published: July 2020

O propósito deste artigo é examinar os tópicos da morte e da doença na obra do memorialista brasileiro Pedro da Silva Nava (1903-1984), sob perspectiva interdisciplinar. O objetivo é discutir essas temáticas, conforme mencionadas em entrevistas e excertos das Memórias, inclusive escritos históricos e biográficos do autor, por meio da análise dos possíveis discursos que estruturam suas concepções de morte e doença. O argumento central do estudo é que Pedro Nava se refere à morte e à doença no âmbito dos temas prioritários, segundo um modelo médico humanista. O arcabouço teórico deste estudo se baseia em obras que versam sobre os principais discursos contemporâneos relativos à doença e à morte. A análise sugere que Pedro Nava explorou as noções de morte e doença com enfoque humanista e sócio-histórico, utilizando seu conhecimento médico como modelo referencial para criar relatos vívidos e retratos biográficos.
ADAMTS7 degrades Comp to fuel BMP2‐dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas
Authors: Wang Chao , Chen Yunqing , Xiang Hongfei , Wu Xiaolin , Tang Qian , Ma Xuexiao , Zhang Lu
Published: July 2020

Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents, with a high metastatic potential. Despite dramatic changes in OS treatments over the past decades, their efficiency still remains limited, with severe complications and adverse side effects. Key mechanisms underlining tumorigenesis, metastasis and chemotherapy resistance are currently lacking, in turn hindering any progress with respect to developing effective and safe therapeutic strategies against OS. Recently, ADAMTS7, a member of the disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family, was shown to be involved in osteogenic differentiation‐related pathological processes. ADAMTS7 promotes vascular calcification via disturbing the balance between osteogenic bone morphogenetic protein (BMP)2 (regulating osteogenic differentiation and bone formation during development) and its natural inhibitor cartilage oligomeric matrix protein (Comp). Hence, in the present study, we aimed to investigate the role of ADAMTS7 in the pathological process of OS. We first revealed that ADAMTS7 was decreased in OS tissues. Lower expression of ADAMTS7 was correlated with poor histological differentiation and an advanced clinical stage of OS. Through loss‐ and gain‐function analysis, we further revealed that ADAMTS7 attenuated cell proliferation, migration and invasion, at the same time as promoting the expression of osteogenic differentiation markers in two OS cell lines: MG63 and SAOS2. Moreover, Comp was responsible for the effects of ADAMTS7 on OS pathogenesis by reinforcing cell osteogenic differentiation mediated by BMP2 in vitro. In conclusion, ADAMTS7‐mediated degradation of Comp may provide a potential therapeutic target for the treatment of OS.
Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo
Authors: Mao Mao , Chang Chun‐Chi , Pickar‐Oliver Adrian , Cervia Lisa D. , Wang Liangli , Ji Jing , Liton Paloma B. , Gersbach Charles A. , Yuan Fan
Published: July 2020

Cell engineering relies heavily on viral vectors for the delivery of molecular cargo into cells due to their superior efficiency compared to nonviral ones. However, viruses are immunogenic and expensive to manufacture, and have limited delivery capacity. Nonviral delivery approaches avoid these limitations but are currently inefficient for clinical applications. This work demonstrates that the efficiency of nonviral delivery of plasmid DNA, mRNA, Sleeping Beauty transposon, and ribonucleoprotein can be significantly enhanced through pretreatment of cells with the nondegradable sugars (NDS), such as sucrose, trehalose, and raffinose. The enhancement is mediated by the incorporation of the NDS into cell membranes, causing enlargement of lysosomes and formation of large (>500 nm) amphisome‐like bodies (ALBs). The changes in subcellular structures redirect transport of cargo to ALBs rather than to lysosomes, reducing cargo degradation in cells. The data indicate that pretreatment of cells with NDS is a promising approach to improve nonviral cargo delivery in biomedical applications.
An Ultrasound‐Excitable Aggregation‐Induced Emission Dye for Enhanced Sonodynamic Therapy of Tumors
Authors: Zeng Weiwei , Xu Yan , Yang Weitao , Liu Kai , Bian Kexin , Zhang Bingbo
Published: July 2020

Ultrasound (US)‐triggered sonodynamic therapy (SDT) can significantly solve the problem of tissue penetrability of light of photodynamic therapy (PDT) that has long vexed physicians in clinics. However, there is a great shortage of sonosensitizers for SDT. Currently, several photosensitizers and their derivatives have been reported for SDT but these dyes are usually quenched when aggregated due to aggregation‐caused quenching (ACQ) effect. In this work, aggregation‐induced emission (AIE) dye (TTMN) assembled nanoparticles (S‐AIE) are synthesized and employed as sonosensitizers for enhanced SDT due to the unique properties of the AIE dye and the deep tissue penetration of ultrasound. Results show that S‐AIE can generate potent singlet oxygen (1O2) under US irradiation to induce cancer cells apoptosis and clearly inhibit tumor growth in vitro and in vivo. In particular, the intrinsic fluorescence of AIE dye can guide the procedure of SDT. To the best of current knowledge, this is the first demonstration of AIE dyes being used as sonosensitizers for SDT and importantly, this work could inspire other more efficient AIE dyes for being used as sonosensitizers for SDT of deep‐seated tumors.
Opto‐E‐Dura: A Soft, Stretchable ECoG Array for Multimodal, Multiscale Neuroscience
Authors: Renz Aline F. , Lee Jihyun , Tybrandt Klas , Brzezinski Maciej , Lorenzo Dayra A. , Cerra Cheraka Mouna , Lee Jaehong , Helmchen Fritjof , Vörös Janos , Lewis Christopher M.
Published: July 2020

Soft, stretchable materials hold great promise for the fabrication of biomedical devices due to their capacity to integrate gracefully with and conform to biological tissues. Conformal devices are of particular interest in the development of brain interfaces where rigid structures can lead to tissue damage and loss of signal quality over the lifetime of the implant. Interfaces to study brain function and dysfunction increasingly require multimodal access in order to facilitate measurement of diverse physiological signals that span the disparate temporal and spatial scales of brain dynamics. Here the Opto‐e‐Dura, a soft, stretchable, 16‐channel electrocorticography array that is optically transparent is presented. Its compatibility with diverse optical and electrical readouts is demonstrated enabling multimodal studies that bridge spatial and temporal scales. The device is chronically stable for weeks, compatible with wide‐field and 2‐photon calcium imaging and permits the repeated insertion of penetrating multielectrode arrays. As the variety of sensors and effectors realizable on soft, stretchable substrates expands, similar devices that provide large‐scale, multimodal access to the brain will continue to improve fundamental understanding of brain function.
A pH‐Sensitive Self‐Assembled and Carrier‐Free Nanoparticle Based on Charge Reversal for Enhanced Synergetic Chemo‐Phototherapy
Authors: Liu Chen , Liu Qiuhong , Chen Luping , Li Mao , Yin Jieli , Zhu Xuan , Chen Dengyue
Published: July 2020

To overcome biological barriers for nanoparticles (NPs) efficaciously accumulated at tumor sites, as well as enhancing the performance of drug delivery systems, a carrier‐free nanoparticle based on charge reversal is designed for improved synergetic chemo‐phototherapy for cancer treatment. In this system, doxorubicin (Dox) and zinc phthalocyanine (ZnPc) are self‐assembled through noncovalent interactions (π–π stacking, hydrophobic forces) to avoid the possible toxicity of excipient, complex chemical conjugations and batch‐to‐batch variation. A trace amount of poly(2‐(di‐methylamino) ethylmethacrylate)‐ poly[(R)‐3‐hydroxybutyrate]‐ poly(2‐(dimethylamino) ethylmethacrylate (PDMAEMA‐PHB‐PDMAEMA) is modified on the surface of Dox–ZnPc to construct the novel nanoparticles, namely DZP, with long‐term stability, and with a dual‐drug load content of up to ≈90％. The drug delivery system (DDS) can effectively decrease its toxicity among physical circulation and increase the accumulation at the tumor site. Moreover, the developed DZP nanoparticles show excellent photo‐chemotherapy, photoacoustic (PA) and fluorescence (FL) imaging characteristics for multimodal imaging‐guided synergetic therapy.
Steering Hollow Multishelled Structures in Photocatalysis: Optimizing Surface and Mass Transport
Authors: Wei Yanze , Yang Nailiang , Huang KeKe , Wan Jiawei , You Feifei , Yu Ranbo , Feng Shouhua , Wang Dan
Published: July 2020

Hollow multishelled structures (HoMSs) provide a promising platform for fabricating photocatalysts, because the unique structure optimizes the effective surface and mass transport, showing enhanced light absorption, optimized mass transport and highly effective active sites exposed. Subsequently, the rational design on HoMS photocatalytsts is elaborated to boost the photocatalytic activity with efforts in all dimensions, from nanoscale to microscale. Breakthroughs in synthetic methodology of HoMSs have greatly evoked the prosperous photocatalytic researches for HoMSs since the developing of sequential templating approach in 2009. The dawn of HoMS photocatalyst is coming after revealing the temporal‐spatial ordering property, which is also discussed in this paper with pioneer works demonstrating the greatly enhanced energy/mass transfer processes. Some insights into the key challenges and perspectives of HoMSs photocatalysts are also discussed. With the reviewed fate and future of HoMSs photocatalysts, hopefully new concepts and innovative works can be inspired to flourish this sun‐rise field.
One‐Pot Tandem Protocol for the Synthesis of 1,3‐Bis(β‐aminoacrylate)‐Substituted 2‐Mercaptoimidazole Scaffolds
Authors: Luo Jian , Chen Guo‐Shu , Chen Shu‐Jie , Li Zhao‐Dong , Zhao Yu‐Lei , Liu Yun‐Lin
Published: July 2020

We have developed a palladium‐catalyzed cross‐coupling reaction of isocyanides with α‐diazoacetates to form active ketenimines and following a 1,4‐diazabicyclo[2.2.2]octane (DABCO) catalyzed aza‐Mannich type reaction with various 2‐mercaptoimidazoles for the synthesis of 1,3‐bis(β‐aminoacrylate)‐substituted 2‐mercaptoimidazole derivatives with structural diversity. In addition, the use of 1H‐benzo[d]imidazol‐2‐ol as the reaction partner also allowed the formation of 1,3‐bis(β‐aminoacrylate)‐substituted 2‐benzimidazolinone derivatives with moderate yields (34–52%). In the aza‐Mannich reaction, the regioselective N‐attack rather than S‐attack or O‐attack at the electrophilic central carbon of ketenimines was observed. This tandem sequence is efficient since two C=C and two C−N bonds are consecutively created in one‐pot.

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A Macromolecule Reversing Antibiotic Resistance Phenotype and Repurposing Drugs as Potent Antibiotics
Authors: Ding Xin , Yang Chuan , Moreira Wilfried , Yuan Peiyan , Periaswamy Balamurugan , de Sessions Paola Florez , Zhao Huimin , Tan Jeremy , Lee Ashlynn , Ong Kai Xun , Park Nathaniel , Liang Zhen Chang , Hedrick James L. , Yang Yi Yan
Published: July 2020

In order to mitigate antibiotic resistance, a new strategy to increase antibiotic potency and reverse drug resistance is needed. Herein, the translocation mechanism of an antimicrobial guanidinium‐functionalized polycarbonate is leveraged in combination with traditional antibiotics to afford a potent treatment for drug‐resistant bacteria. Particularly, this polymer–antibiotic combination approach reverses rifampicin resistance phenotype in Acinetobacter baumannii demonstrating a 2.5 × 105‐fold reduction in minimum inhibitory concentration (MIC) and a 4096‐fold reduction in minimum bactericidal concentration (MBC). This approach also enables the repurposing of auranofin as an antibiotic against multidrug‐resistant (MDR) Gram‐negative bacteria with a 512‐fold MIC and 128‐fold MBC reduction, respectively. Finally, the in vivo efficacy of polymer–rifampicin combination is demonstrated in a MDR bacteremia mouse model. This combination approach lays foundational ground rules for a new class of antibiotic adjuvants capable of reversing drug resistance phenotype and repurposing drugs against MDR Gram‐negative bacteria.
Synthesis of Thiol‐Containing Oligopeptides via Tandem Activation of γ‐Thiolactones by Silver‐DABCO Pair
Authors: Jiang Sheng‐Yuan , Hsieh Wen‐Tsai , Chen Wei‐Shuo , Liao Jia‐Shiang , Chiang Po‐Yu , Lin Yuya A.
Published: July 2020

A method involving tandem activation of γ‐thiolactone by silver‐DABCO pair has been developed to enable successful amidation of homocysteine thiolactone by various amino acid residues and dipeptides with yields up to 95%. The introduction of thiol functionality to peptides directly using cysteine or homocysteine would require the protection of the reactive sulfhydryl groups and deprotection at a later stage of the synthesis. The γ‐thiolactone chemistry presented herein shows that it could serve as a masked and activatable reaction handle to prepare a range of thiol‐containing peptides in a more atom‐economical way. The resulting thiol‐containing peptides could facilitate late‐stage functionalization and structural diversification of functional or bioactive peptides. The silver(I) ion was found to be essential for the activation of the γ‐thiolactone, poor reactions were observed otherwise. A tentative mechanism for the silver‐DABCO promoted γ‐thiolactone aminolysis was proposed and supported by the evidence from thermal desorption electrospray ionization mass spectrometry.
Effect of injection molding on structure and properties of poly(styrene‐ethylene‐butylene‐styrene) and its nanocomposite with functionalized montmorillonite
Authors: Li Xiaoyan , Yu Hui , Kang Xiong , Chen Gang , Zhu Ming , Xu Jianjun
Published: July 2020

In this paper, the effects of injection molding on structure and properties of poly(styrene‐ethylene‐butylene‐styrene) (SEBS) and its nanocomposite with a functionalized montmorillonite (FMMT) were illustrated by comparison with the results of hot pressing. The injection‐molded SEBS and its FMMT nanocomposite have a skin‐core morphology with FMMT platelets more preferentially aligned along the sample plane, while hot‐pressed samples not. The injection‐molded samples also have a dense structure, and a less extent of microphase separation. In the injection molding process, the high shear flow coupled with solidification in presence of temperature gradient and high pressure in the closed cavity greatly affect the morphology and structure of the SEBS and its nanocomposites. The results also indicate attractive interaction between the soft segments of SEBS and the long alkyl silane chain grafted to FMMT, which allows FMMT to form a macroscopic network structure after the high‐speed and high‐pressure injection molding. The SEBS/FMMT‐injected nanocomposite have the highest tensile strength and the best corrosion resistance, which can be attributed to the dense skin‐core structure, stronger SEBS/FMMT interaction and the preferentially aligned FMMT platelets.
Time series of optical spectra of Nova V659 Sct
Authors: Jack Dennis , Schröder Klaus‐Peter , Eenens Philippe , Wolter Uwe , González‐Pérez José Nicolás , Schmitt Jürgen H. M. M. , Hauschildt Peter H.
Published: July 2020

With our robotic 1.2 m TIGRE telescope, we were able to obtain eight optical spectra with intermediate resolution (R ≈ 20,000) of the Nova V659 Sct during different phases of its outburst. We present a list of the lines found in the Nova spectra. The most common features are H I, O I, Na I, Fe II, and Ca II. Studying the spectral evolution of the strong features, we found that the absorption features move to higher expansion velocities before disappearing, and the emission features show (different) asymmetries. Because of the intermediate spectral resolution, we identified and analyzed the interstellar medium absorption features present in the spectra. We detected atomic absorption features of Na I and Ca II. The sodium D lines show more complex substructures with three main absorption features at a velocity of around −10, 30, and 85 km s−1. We identified several diffuse interstellar bands (DIBs) in the Nova V659 Sct spectra and determined their velocities and equivalent widths.
Bisphenols and phthalates: Plastic chemical exposures can contribute to adverse cardiovascular health outcomes
Authors: Ramadan Manelle , Cooper Blake , Posnack Nikki Gillum
Published: July 2020

Phthalates and bisphenols are high production volume chemicals that are used in the manufacturing of consumer and medical products. Given the ubiquity of bisphenol and phthalate chemicals in the environment, biomonitoring studies routinely detect these chemicals in 75–90% of the general population. Accumulating evidence suggests that such chemical exposures may influence human health outcomes, including cardiovascular health. These associations are particularly worrisome for sensitive populations, including fetal, infant and pediatric groups—with underdeveloped metabolic capabilities and developing organ systems. In the presented article, we aimed to review the literature on environmental and clinical exposures to bisphenols and phthalates, highlight experimental work that suggests that these chemicals may exert a negative influence on cardiovascular health, and emphasize areas of concern that relate to vulnerable pediatric groups. Gaps in our current knowledge are also discussed, so that future endeavors may resolve the relationship between chemical exposures and the impact on pediatric cardiovascular physiology.
Stakeholder engagement in a sustainable sales and operations planning process
Authors: Roscoe Samuel , Subramanian Nachiappan , Prifti Romina , Wu Lin
Published: July 2020

The purpose of this paper is to understand how companies can transition to a sustainable sales and operations planning process to improve economic, environmental, and social performance. Data are collected from a survey of 120 managers in China's manufacturing sector and analyzed using partial least square‐based structural equation modeling. Drawing on life cycle theory and stakeholder theory, we argue that the conventional sales and operations planning (S&OP) process is internally focused and myopic, which over time leads to path dependencies and structural inertia. We find that firms can break free from this structural inertia by engaging external stakeholder groups that challenge the status quo and prompt organizational change. The paper contributes to theory by combining the key tenets of life cycle theory and stakeholder theory to explain how companies can transition to a sustainable S&OP process.
Promising insights into the kosmotropic effect of magnetic nanoparticles on proteins: The pivotal role of protein corona formation
Authors: Fattah Reza , Rashedi Hamid , Yazdian Fatemeh , Mousavi Seyed Babak , Fazeli Ahmad
Published: July 2020

Increasing concerns about biosafety of nanoparticles (NPs) has raised the need for detailed knowledge of NP interactions with biological molecules especially proteins. Herein, the concentration‐dependent effect of magnetic NPs (MNPs) on bovine serum albumin and hen egg white lysozyme was explored. The X‐ray diffraction patterns, zeta potential, and dynamic light scattering measurements together with scanning electron microscopy images were employed to characterize MNPs synthesized through coprecipitation method. Then, we studied the behavior of two model proteins with different surface charges and structural properties on interaction with Fe3O4. A thorough investigation of protein–MNP interaction by the help of intrinsic fluorescence at different experimental conditions revealed that affinity of proteins for MNPs is strongly affected by the similarity of protein and MNP surface charges. MNPs exerted structure‐making kosmotropic effect on both proteins under a concentration threshold; however, binding strength was found to determine the extent of stabilizing effect as well as magnitude of the concentration threshold. Circular dichroism spectra showed that proteins with less resistance to conformational deformations are more prone to secondary structure changes upon adsorption on MNPs. By screening thermal aggregation of proteins in the presence of Fe3O4, it was also found that like chemical stability, thermal stability is influenced to a higher extent in more strongly bound proteins. Overall, this report not only provides an integrated picture of protein–MNP interaction but also sheds light on the molecular mechanism underling this process.
Raman‐based dynamic feeding strategies using real‐time glucose concentration monitoring system during adalimumab producing CHO cell cultivation
Authors: Domján Júlia , Fricska Annamária , Madarász Lajos , Gyürkés Martin , Köte Ákos , Farkas Attila , Vass Panna , Fehér Csaba , Horváth Balázs , Könczöl Kálmán , Pataki Hajnalka , Nagy Zsombor Kristóf , Marosi György János , Hirsch Edit
Published: July 2020

The use of Process Analytical Technology tools coupled with chemometrics has been shown great potential for better understanding and control of mammalian cell cultivations through real‐time process monitoring. In‐line Raman spectroscopy was utilized to determine the glucose concentration of the complex bioreactor culture medium ensuring real‐time information for our process control system. This work demonstrates a simple and fast method to achieve a robust partial least squares calibration model under laboratory conditions in an early phase of the development utilizing shake flask and bioreactor cultures. Two types of dynamic feeding strategies were accomplished where the multi‐component feed medium additions were controlled manually and automatically based on the Raman monitored glucose concentration. The impact of these dynamic feedings was also investigated and compared to the traditional bolus feeding strategy on cellular metabolism, cell growth, productivity, and binding activity of the antibody product. Both manual and automated dynamic feeding strategies were successfully applied to maintain the glucose concentration within a narrower and lower concentration range. Thus, besides glucose, the glutamate was also limited at low level leading to reduced production of inhibitory metabolites, such as lactate and ammonia. Consequently, these feeding control strategies enabled to provide beneficial cultivation environment for the cells. In both experiments, higher cell growth and prolonged viable cell cultivation were achieved which in turn led to increased antibody product concentration compared to the reference bolus feeding cultivation.
Gene signature‐based prediction of triple‐negative breast cancer patient response to Neoadjuvant chemotherapy
Authors: Zhao Yanding , Schaafsma Evelien , Cheng Chao
Published: July 2020

Neoadjuvant chemotherapy is the current standard of care for large, advanced, and/or inoperable tumors, including triple‐negative breast cancer. Although the clinical benefits of neoadjuvant chemotherapy have been illustrated through numerous clinical trials, more than half of the patients do not experience therapeutic benefit and needlessly suffer from side effects. Currently, no clinically applicable biomarkers are available for predicting neoadjuvant chemotherapy response in triple‐negative breast cancer; the discovery of such a predictive biomarker or marker profile is an unmet need. In this study, we introduce a generic computational framework to calculate a response‐probability score (RPS), based on patient transcriptomic profiles, to predict their response to neoadjuvant chemotherapy. We first validated this framework in ER‐positive breast cancer patients and showed that it predicted neoadjuvant chemotherapy response with equal performance to several clinically used gene signatures, including Oncotype DX and MammaPrint. Then, we applied this framework to triple‐negative breast cancer data and, for each patient, we calculated a response probability score (TNBC‐RPS). Our results indicate that the TNBC‐RPS achieved the highest accuracy for predicting neoadjuvant chemotherapy response compared to previously proposed 143 gene signatures. When combined with additional clinical factors, the TNBC‐RPS achieved a high prediction accuracy for triple‐negative breast cancer patients, which was comparable to the prediction accuracy of Oncotype DX and MammaPrint in ER‐positive patients. In conclusion, the TNBC‐RPS accurately predicts neoadjuvant chemotherapy response in triple‐negative breast cancer patients and has the potential to be clinically used to aid physicians in stratifying patients for more effective neoadjuvant chemotherapy.
A Modified Technique of Tacking Acellular Dermal Matrix to Increase Keratinized Mucosa Around Dental Implants as an Alternative to a Free Gingival Graft: A Case Report
Authors: Shi Ye , Segelnick Stuart L. , El Chaar Edgard S.
Published: July 2020

Introduction

Free autogenous graft was the gold standard to increase the keratinized mucosa (KM) and vestibular depth. The major downfall of this technique is the postoperative morbidity at the donor site. The purpose of this case report is to demonstrate a modified technique using acellular dermal matrix (ADM) to increase the KM around implants to achieve faster healing with less postoperative morbidity.

Case Presentation

Patient presented with inadequate keratinized tissue band and shallow vestibule at submerged implant sites bilaterally. Initially, surgical procedure of vestibuloplasty in conjunction with free gingival graft (FGG) was performed at one side. However, patient opted for the allograft as a substitute for the other side due to the postoperative discomfort from palatal donor site. On the left side, the FGG procedure was performed in a conventional way stabilizing with sutures. On the right side, the ADM was stabilized with tacks only at recipient site and left exposed. The new vestibule was established and stabilized with tacks.  A significant gain of KM and vestibule depth was observed at the site of using ADM when compared with baseline. For the site of using FGG, KM was increased. However, the vestibule was rebounded compared with the site of using ADM with tacks.

Conclusions

The use of ADM stabilized with tacks is a predictable procedure that can increase KM and establish stable vestibule around dental implants. It can lead to less chair time, faster healing, and reduced postoperative morbidity compared with autogenous soft tissue graft.
Destigmatising the stigma: Understanding the impact of message framing on Chinese consumers' guilt and attitude associated with overspending behaviour
Authors: Fan Wuqiu , Zhong Hanchun , Zhu Anding
Published: July 2020

Overspending has been a largely stigmatised consumer behaviour in traditional Chinese culture. However, social and economic development in recent decades has induced the rise of Chinese consumerism and changes in public attitudes towards overspending. Focusing on overspending stigmas Gouwukuang (GWK) and Duoshoudang (DSD) and stigma‐relevant attitudes, the present study investigates the effect of perceived stigma on consumers' attitude towards overspending through the mediating effect of anticipated consumer guilt. The moderating role of message framing in this relationship is also examined. Results of two analyses indicate that (a) between the two stigmas, DSD is less stigmatising and preferred in comparison to GWK in social interaction and self‐identification; (b) participants' perception of the stigma associated with overspending predicts their anticipation of guilt associated with this behaviour, which in turn predicts negative attitude towards overspending; (c) message framing moderates the relationship between perceived stigma and anticipated guilt, and hence facilitates destigmatisation of the traditionally stigmatised behaviour of overspending. Theoretical and practical implications of these findings are discussed.
Design, Synthesis and Biological Evaluation of Xanthone Derivatives for Possible Treatment of Alzheimer's Disease Based on Multi‐Target Strategy
Authors: Yang Aihong , Yu Qiao , Ju Hui , Song Lulu , Kou Xiaodi , Shen Rui
Published: July 2020

Four xanthone derivatives were synthesized and evaluated as acetylcholinesterase inhibitors (AChEIs) with metal chelating ability and antioxidant ability against Alzheimer's disease (AD). Most of them exhibited potential acetylcholinesterase (AChE), butylcholinesterase (BuChE) inhibitory, antioxidant and metal chelating properties. Among them, 1‐hydroxy‐3‐[2‐(pyrrolidin‐1‐yl)ethoxy]‐9H‐xanthen‐9‐one had the highest ability to inhibit AChE and displayed high selectivity towards AChE (IC50=2.403±0.002 μM for AChE and IC50=31.221±0.002 μM for BuChE), and it was also a good antioxidant (IC50=2.662±0.003 μM). Enzyme kinetic studies showed that this compound was a mixed‐type inhibitor, which could interact simultaneously with the catalytic anionic site (CAS) and the peripheral anionic site (PAS) of AChE. Interestingly, its copper complex showed more significant inhibitory activity for AChE (IC50=0.934±0.002 μM) and antioxidant activity (IC50=1.064±0.003 μM). Molecular dockings were carried out for the four xanthone derivatives in order to further investigate the binding modes. Finally, the blood‐brain barrier (BBB) penetration prediction indicated that all compounds might penetrate BBB. These results suggested that 1‐hydroxy‐3‐[2‐(pyrrolidin‐1‐yl)ethoxy]‐9H‐xanthen‐9‐one was promising AChEI with metal chelating ability and antioxidant ability for the further investigation.
Pulsed radiofrequency as alternative method for phantom pain treatment. Case report
Authors: Brzeziński Krzysztof , Rękas‐Dudziak Anna Renata , Maruszewska Agnieszka
Published: July 2020

Pulse radiofrequency is a safe method of fighting phantom pain. It creates the possibility of treating cases that have exhausted other therapeutic options.
Advances on the Use of Graphene as a Label for Electrochemical Biosensors
Authors: Bonanni Alessandra
Published: July 2020

There has been an overwhelming interest in the use of graphene and its derivatives for electrochemical biosensors in the last decade. Although the majority of the works describe the use of these materials as platform for the immobilization of the biorecognition element, there is a significant, often unrecognized, research effort that has shown how graphene materials can also beneficially serve as signaling labels for biosensing. Owing to its intrinsic electroactivity and small size, nano‐graphene oxide, for example, has been used for this purpose, where the working signal arises from the reduction of the oxygen functionalities on the material surface. In other approaches, graphene labels modified with electroactive probes were also used to promote the signal generation. This Minireview will illustrate how the unique electrochemical and structural features make graphene a very promising material for the detection of the biorecognition event. In addition, an overview will be provided, showing the plethora of options offered by graphene and its derivatives as labels for signal generation and enhancement.
Tuning the Ground and Excited State Dynamics of Hemithioindigo Molecular Motors by Changing Substituents
Authors: Wilcken Roland , Huber Ludwig , Grill Kerstin , Guentner Manuel , Schildhauer Monika , Thumser Stefan , Riedle Eberhard , Dube Henry
Published: July 2020

Efficiency and performance of light triggered molecular motors are crucial features that need to be mechanistically understood to improve the performance and enable conscious property tailoring for specific applications. In this work, three different hemithioindigo‐based molecular motors are investigated and all four steps in their complete unidirectional rotation are unraveled fully quantitatively. Transient absorption spectroscopy across twelve orders of magnitude in time is used to probe the fs nuclear motions up to the ms thermal kinetics, covering the timeframe of the whole motor rotation. The newly known full mechanisms allow simulation of the motor systems to scrutinize their performance at realistic illumination conditions. This highlights the importance of photoisomerization quantum yields for the rotation speed. The substitution pattern in close proximity to the rotation axle influences the excited and ground state properties. Reduction of electron donation and concomitant increase of steric hindrance leads to faster photoisomerization reactions with quasi‐ballistic behavior, but also to a slight decrease in the quantum efficiency. The expected decelerating effects of increased sterics are primarily manifested in the ground state. A promising approach for next‐generation hemithioindigo motors is to elevate electron donation at the rotor fragment followed by an increase of steric hindrance.
Observation of hyperpositive non‐linear effect in catalytic asymmetric organozinc additions to aldehydes
Authors: Geiger Yannick , Achard Thierry , Maisse‐François Aline , Bellemin‐Laponnaz Stéphane
Published: July 2020

Asymmetric amplification is a phenomenon that is believed to play a key role in the emergence of homochirality in life. In asymmetric catalysis, theoretical and experimental models have been investigated to provide an understanding of how chiral amplification is possible, in particular based on non‐linear effects. Interestingly, it has been proposed a quarter century ago that chiral catalysts, when not enantiopure might even be more enantioselective than their enantiopure counterparts. We show here that such hyperpositive non‐linear effect in asymmetric catalysis is indeed possible. An in‐depth study into the underlying mechanism was carried out, and the scheme we derive differs from the previous proposed models.
Art of musical color: A synesthesia‐based mechanism of color art
Authors: Hu Guosheng
Published: July 2020

Existing music‐color synesthesia projects fundamentally focus on musical pitch and color, but seldom touch on music structure. This article proposes a systematic color visualization method of visual language based on music‐color synesthesia. Not only does it involve pitch‐color with timbres of various instruments but also adopts musical elements (even structures) in plastic units of color composition. The author established a metaphorical language of musical color based on the synesthesia theory. Quantizing‐oriented as this language is, generative art and interactive system of color can be developed. It could also be applied in visualized music education and as a synesthetic performance aid.
The supervisory relationship from an attachment perspective: Connections to burnout and sense of coherence in health professionals
Authors: Hiebler‐Ragger Michaela , Nausner Liselotte , Blaha Anna , Grimmer Karl , Korlath Silvia , Mernyi Margarete , Unterrainer Human F.
Published: July 2020

Supervision is an essential part of the training and work environment of health professionals, especially of psychotherapists and clinical/health psychologists. However, although the supervisory process is always a relational one and may therefore be influenced by attachment dynamics, the importance of the supervisory relationship for the professional's performance and the well‐being of the supervisee has yet to be fully examined.

In this cross‐sectional observational study, the Experiences in Close Relationships‐Revised (ECR‐RD; avoidant and anxious attachment), the Supervisory Relationship Questionnaire (SRQ), the Maslach Burnout Inventory (MBI) and the Sense of Coherence Scale (SOC‐13) were analysed for 346 (81.8% female) health professionals.

Considering professional experience and number of supervision sessions as control variables, a better supervisory relationship negatively predicted burnout symptoms (β = −.31) but positively predicted sense of coherence (β = .31, both p < .01). The final model, including avoidant and anxious attachment as additional predictors, explained 30% of the variance in burnout symptoms and 41% of the variance in sense of coherence.

The results underline the importance of the supervisory relationship for the well‐being and the professional performance of health professionals. Interactions between the supervisory relationship and underlying attachment parameters should be further explored in future studies.
A fuzzy multicriteria decision‐making‐based CH selection and hybrid routing protocol for WSN
Authors: Sreedharan Panchikattil Susheelkumar , Pete Dnyandeo Jageshwar
Published: July 2020

One of the famous approaches to decision making is named as multicriteria decision making (MCDM). In order to solve the MCDM issues, a better way is provided by the fuzzy logic. Expendability, cost, maintenance, availability of software, and performance characteristics are such problems considered by the decision. The precise estimation of the pertinent data is one of the vital phases in DM systems. This paper presents a fuzzy MCDM‐based cluster head (CH) selection and hybrid routing protocol to solve the most common issues. In this research article, the generalized intuitionistic fuzzy soft set (GIFSS) approach is utilized to select the optimal CH, and hybrid shark smell optimization (SSO), and a genetic algorithm (GA) is introduced for the effective routing. Initially, the wireless sensor network (WSN) system and energy models are designed, and then the nodes are grouped into several clusters. Next, based on the GIFSS, the CH nodes are selected, and finally, an effective routing is placed based on the hybrid optimizations. The implementation is performed on the NS2 platform, and the performances are evaluated by packet delivery ratio (PDR), delay, packet loss ratio (PLR), network lifetime, bit error rate (BER), energy consumption, throughput, and jitter. The existing approaches named energy centers examining using particle swarm optimization (EC‐PSO), variable dimension‐based PSO (VD‐PSO), energy‐efficient PSO‐based CH selection (PSO‐ECHS), low‐energy adaptive clustering hierarchy‐sugeno fuzzy (LEACH‐SF), SSO, and GA are compared with the proposed strategy. According to the implemented outcomes, it displays the proposed strategy and gives improved outcomes than the others.
Review your agreements with online course providers to ensure FERPA compliance
Authors: Gelpi Aileen
Published: July 2020

Thank goodness the spring 2020 term is over! If your institution plans to evaluate its response to the COVID‐19 emergency that led to the mass migration of in‐person education to online classes, why not assess whether the method of delivery complies with the Family Educational Rights and Privacy Act? The following issues can be the starting point for your institution's evaluation.
Connect new students to your institution through online orientation
Authors: Sutton Halley
Published: July 2020

With the COVID‐19 pandemic, colleges and universities nationwide were forced to unexpectedly move courses online to finish out the spring 2020 semester. Meg Foster, First Year Experience Instructor and Academic Instructor and Coach at Piedmont Virginia Community College, shared best practices for crafting online orientations to engage students in a complimentary webinar hosted by Innovative Educators.
Why I recovered: A qualitative investigation of factors promoting motivation for eating disorder recovery
Authors: Venturo‐Conerly Katherine E. , Wasil Akash R. , Dreier Melissa J. , Lipson Sarah M. , Shingleton Rebecca M. , Weisz John R.
Published: July 2020

Objective

It is difficult for individuals with eating disorders (EDs) to build and maintain motivation to recover. This challenge contributes to high rates of treatment dropout and relapse. To date, motivational interventions have been largely ineffective, and there is little research on factors that affect recovery motivation. To better understand recovery motivation and identify potential intervention targets, this study examines factors that affect recovery motivation in individuals with EDs.

Method

N = 13 participants completed qualitative interviews. All had been recovered from their diagnosed and treated ED for at least 1 year. We applied thematic analysis to interview transcripts in order to identify factors that had influenced recovery motivation and to classify their effects as helpful, harmful, or mixed.

Results

Six main themes were identified, with subthemes detailed under each: (a) important people and groups (e.g., social circle, mentor), (b) actions and attitudes of others (e.g., judgmental responses, failure to intervene), (c) treatment‐related factors (e.g., therapeutic skills, therapeutic alliance), (d) influential circumstances (e.g., removing triggers, pregnancy/children), (e) personal feelings and beliefs (e.g., obligation to others, hope for the future), and (f) the role of epiphanies (i.e., sudden insights or moments of change).

Discussion

In this study, we identified potentially malleable factors that may affect ED recovery motivation (e.g., removing triggers, focusing on obligation to others, getting involved in meaningful causes, securing non‐judgmental support, building hope for the future). These factors may be investigated as potential targets or strategies in motivational interventions for EDs.
Abundance data for invertebrate assemblages from intertidal mussel beds along the Atlantic Canadian coast
Authors: Scrosati Ricardo A. , Arribas Lorena P. , Donnarumma Luigia
Published: July 2020

This data set describes the abundance of 50 invertebrate taxa found in intertidal mussel beds along the Atlantic Canadian coast. This information resulted from a regional‐scale study that investigated the effects of wave exposure on the richness and composition of invertebrate assemblages from intertidal mussel beds. Abundance data are provided for taxa representing the Annelida, Arthropoda, Bryozoa, Chordata, Cnidaria, Echinodermata, Mollusca, Nematoda, Nemertea, and Platyhelminthes. The data characterize mussel beds from wave‐sheltered and wave‐exposed locations spanning 315 km of the coast of Nova Scotia. Univariate and multivariate analyses revealed that the compositional structure of these invertebrate assemblages differed markedly depending on wave exposure. Overall, because of its taxonomic diversity, the inclusion of data for basal, intermediate, and top trophic levels, and the coverage of two extremes of environmental stress, this data set could be useful to test broader aspects of ecological theory. Areas of ecology that could advance using this data set are those concerning environmental stress models of community organization, abundance–occupancy relationships, species co‐occurrence, species abundance distributions, dominance and rarity, spatial scales of population and community variation, and distribution of functional and phylogenetic diversity. Use of this data set for academic or educational purposes is allowed as long as the data source is properly cited. When used for academic or educational purposes, this data set should be cited using the title of this Data Paper, the names of the authors, the year of publication, and the corresponding volume and article numbers.