bluuebunny/crossref_metadata_2025 huggingface dataset:
Incidence and Predictors of Clinically Significant Bleedings after Transcatheter Left Atrial Appendage Closure
Authors: Zieliński Kamil , Pracoń Radosław , Konka Marek , Kruk Mariusz , Kępka Cezary , Trochimiuk Piotr , Dębski Mariusz , Kaczmarska Edyta , Przyłuski Jakub , Kowalik Ilona , Dzielińska Zofia , Kurowski Andrzej , Witkowski Adam , Demkow Marcin
Published: October 2022

Background: Transcatheter left atrial appendage closure (LAAC) is performed in patients unsuitable for long-term anticoagulation, predominantly due to prior bleeding events. The study aimed to investigate the incidence and predictors of clinically significant bleeding (CSB) post-LAAC. Methods: Consecutive patients after LAAC with an Amplatzer or WATCHMAN device were analyzed (05.2014–11.2019). Bleeding was classified as CSB when associated with at least one of the following: death, ≥2 g/dL hemoglobin drop, ≥2 blood units transfusion, critical anatomic site, or hospitalization/invasive procedure. Results: Among 195 patients (age 74 (68–80), 43.1% females, HAS-BLED score 2.0 (2.0–3.0)), during median follow-up of 370 (IQR, 358–392) days, there were 15 nonprocedural CSBs in 14 (7.2%) patients. Of those, 9 (60.0%) occurred during postprocedural dual antiplatelet therapy (DAPT) (median 46 (IQR: 16–60) days post-LAAC) vs. 6 (40%) after DAPT discontinuation (median 124 (81–210) days post-LAAC), translating into annualized CSB rates of 14.0% (per patient-year on DAPT) vs. 4.6% (per patient-year without DAPT). In 92.9% (13/14) of patients, the post-LAAC nonprocedural CSB was a recurrence from the same site as bleeding pre-LAAC. In the multivariable model, admission systolic blood pressure (SBP) > 127 mmHg (HR = 10.73, 1.37–84.26, p = 0.024), epistaxis history (HR = 5.84, 1.32–25.89, p = 0.020), permanent atrial fibrillation (AF) (HR = 4.55, 1.20–17.20, p = 0.025), and prior gastrointestinal bleeding (HR = 3.35, 1.01–11.08, p = 0.048) predicted post-LAAC CSB. Conclusions: Nonprocedural CSBs after LAAC, with a similar origin as the pre-LAAC bleedings, were observed predominantly during postprocedural DAPT and predicted by elevated admission SBP, prior epistaxis, permanent AF, and gastrointestinal bleeding history. Whether a more reserved post-LAAC antiplatelet regimen and stringent blood pressure control may improve LAAC outcomes remains to be studied.
Clinically Relevant β-Lactam Resistance Genes in Wastewater Treatment Plants
Authors: Waśko Izabela , Kozińska Aleksandra , Kotlarska Ewa , Baraniak Anna
Published: October 2022

Antimicrobial resistance (AMR) is one of the largest global concerns due to its influence in multiple areas, which is consistent with One Health’s concept of close interconnections between people, animals, plants, and their shared environments. Antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) circulate constantly in various niches, sediments, water sources, soil, and wastes of the animal and plant sectors, and is linked to human activities. Sewage of different origins gets to the wastewater treatment plants (WWTPs), where ARB and ARG removal efficiency is still insufficient, leading to their transmission to discharge points and further dissemination. Thus, WWTPs are believed to be reservoirs of ARGs and the source of spreading AMR. According to a World Health Organization report, the most critical pathogens for public health include Gram-negative bacteria resistant to third-generation cephalosporins and carbapenems (last-choice drugs), which represent β-lactams, the most widely used antibiotics. Therefore, this paper aimed to present the available research data for ARGs in WWTPs that confer resistance to β-lactam antibiotics, with a particular emphasis on clinically important life-threatening mechanisms of resistance, including extended-spectrum β-lactamases (ESBLs) and carbapenemases (KPC, NDM).
Survey of Candidatus Liberibacter Solanacearum and Its Associated Vectors in Potato Crop in Spain
Authors: Asensio-S.-Manzanera M. Carmen , Santiago-Calvo Yolanda , Palomo-Gómez José Luis , Marquínez-Ramírez Raquel , Bastin Saskia , García-Méndez Eva María , Hernández-Suárez Estrella , Siverio-de-la-Rosa Felipe
Published: October 2022

‘Candidatus Liberibacter solanacearum’ (CaLsol), the etiological agent of potato zebra chip (ZC), is transmitted to potato plants by the psyllid Bactericera cockerelli (Šulc, 1909) in North and Central America and New Zealand. The risk of the dispersion of ZC in Spain depends on the presence of an efficient vector. This work studies the presence and abundance of ZC symptoms and CaLsol in potato plants, as well as the presence and abundance of psyllid species associated with potato crops in the main producing areas in Spain. Eighty-eight plots were surveyed punctually to detect ZC symptoms and psyllid species in the main potato-producing areas. Furthermore, fourteen potato plots were surveyed by different sampling methods during the cropping season to detect psyllid species from 2016 to 2018. Very few symptomatic and CaLsol-positive plants were detected in Mainland Spain, and any positive plant was detected in the Canary Islands. Most of the adult psyllids captured were identified as Bactericera nigricornis (Foerster, 1848), and some of them as Bactericera trigonica, but no B. cockerelli was detected. B. nigricornis was found widely distributed in the northern half of the Iberian Peninsula; however, this psyllid does not seem sufficient to pose a threat to potato production, due to the scarce number of specimens and because the frequency of B. nigricornis specimens that were CaLsol+ was very low.
Nutritional Composition of Some Commonly Available Aquatic Edible Insects of Assam, India
Authors: Sarmah Mintu , Bhattacharyya Badal , Bhagawati Sudhansu , Sarmah Kritideepan
Published: October 2022

The nutritive value of five edible aquatic insects of Assam—Hemipterans; water bug (Diplonychus rusticus Fabricius) family belostomatidae; giant water bug (Lethocerus indicus Lepeletier and Serville) family belostomatidae; water scorpion (Laccotrephes sp.) family nepidae, water stick (Ranatra sp.) family nepidae; Coleopterans diving beetle (Cybister sp.) family dytiscidae—based on their proximate and elemental composition, antioxidant and antinutritional properties were assessed by using standard methods of analysis. Analytical studies revealed that the selected aquatic insect species have high nutritive value and are rich sources of protein (50.03 to 57.67%) and other nutrients (fat, carbohydrate and crude fiber, etc.) along with superior energy contents (331.98 to 506.38 kJ/100 g). The aquatic insect species also contained appreciable amounts of major and trace dietary elements. Phenol and flavonoid contents reflect its high antioxidant activity (80.82 to 91.47% DPPH inhibition). Tannin (18.50 to 60.76 mg tannic acid equivalent/100 g), phytic acid (11.72 to 97.30 mg/100 g) and oxalic acid (2.93 to 5.34 mg/100 g) as antinutritional compounds were registered below the toxic level (0.52% or 520 mg/100 g). The present findings indicate that the selected aquatic insect species can be considered as ideal candidates for exploration as food and feed to ensure nutritional and livelihood security of this region.
Approach to Knee Arthropathy through 180-Degree Immersive VR Movement Visualization in Adult Patients with Severe Hemophilia: A Pilot Study
Authors: Ucero-Lozano Roberto , Pérez-Llanes Raúl , López-Pina José Antonio , Cuesta-Barriuso Rubén
Published: October 2022

(1) Background: Hemarthrosis is a typical clinical manifestation in patients with hemophilia. Its recurrence causes hemophilic arthropathy, characterized by chronic joint pain. Watching movement recorded from a first-person perspective and immersively can be effective in the management of chronic pain. The objective of this study was to evaluate the effectiveness of an immersive virtual reality intervention in improving the pain intensity, joint condition, muscle strength and range of motion in patients with hemophilic knee arthropathy. (2) Methods: Thirteen patients with hemophilic knee arthropathy were recruited. The patients wore virtual reality glasses and watched a flexion–extension movement of the knee on an immersive 180° video, recorded from a first-person perspective over a 28-day period. The primary variable was the pain intensity (visual analog scale). The secondary variables were the joint status (Hemophilia Joint Health Score), quadriceps and hamstring strength (dynamometry), and range of motion (goniometry). (3) Results: After the intervention period, statistically significant differences were observed in the intensity of the joint pain (Standard error [SE] = 19.31; 95% interval confidence [95%CI] = −1.05; −0.26), joint condition (SE = 18.68; 95%CI = −1.16; −0.52) and quadriceps strength (SE = 35.00; 95%CI = 2.53; 17.47). We found that 38.46% and 23.07% of the patients exhibited an improvement in their quadriceps muscle strength and joint condition above the minimum detectable change for both variables (8.21% and 1.79%, respectively). (4) Conclusions: One hundred and eighty degree immersive VR motion visualization can improve the intensity of joint pain in patients with hemophilic knee arthropathy. An intervention using immersive virtual reality can be an effective complementary approach to improve the joint condition and quadriceps strength in these patients.
Robot-Assisted versus Trans-Umbilical Multiport Laparoscopic Ureteral Reimplantation for Pediatric Benign Distal Ureteral Stricture: Mid-Term Results at a Single Center
Authors: Guo Yanhua , Cheng Yang , Li Dan , Mei Hong , Jin Huan , Chen Guo , Hu Anpei , Li Qilan , Du Xinyi , Zheng Liduan , Tong Qiangsong
Published: October 2022

Objective: Robot-assisted laparoscopic ureteral reimplantation (RALUR) and trans-umbilical multiport laparoscopic ureteral reimplantation (TMLUR) are both minimally invasive procedures for benign distal ureteral stricture (DUS). However, TMLUR has rarely been reported in published research, thus the difference in mid-term outcome of these two procedures warrants investigation. Methods: Patients who underwent RALUR or TMLUR for pediatric DUS from April 2017 to November 2020 at our institution were retrospectively analyzed and 56 patients were included in this retrospective comparison. Demographic characteristics, perioperative data and follow-up results were collected and analyzed in RALUR and TALUR groups. Results: RALUR and TMLUR were successfully performed in children aged from 12.0 to 142.0 months, without conversion to open ureteral reimplantation. RALUR took shorter operative time than TMLUR (p = 0.005) with less blood loss (p = 0.001). Meanwhile, patients receiving RALUR encountered a greater financial burden (p < 0.001) with less cosmetic satisfaction than TMLUR. The mean mid-term follow-up time for RALUR and TMLUR was 18.29 months and 24.64 months, respectively. Mid-term follow-up data showed that DUS was relieved with improved renal function after surgery in both groups, with no significant difference. Conclusions: RALUR and TMLUR are both safe and efficient for DUS treatment and achieve comparable mid-term outcomes in children. RALUR can reduce operative time and operative blood loss benefiting from its prominent technical superiority, but may currently bring about greater financial burden, with cosmetic satisfaction remaining to be improved.
B7-H4 Polymorphism Influences the Prevalence of Diabetes Mellitus and Pro-Atherogenic Dyslipidemia in Patients with Psoriasis
Authors: Yang Wenjing , Huang Qiong , Han Ling , Wang Bing , Yawalkar Nikhil , Zhang Zhenghua , Yan Kexiang
Published: October 2022

Background: The co-inhibitory molecule B7-H4 is located in the genomic regions associated with type 1 diabetes (T1D) susceptibility. However, the correlation of B7-H4 with glycometabolism and dyslipidemia has never been studied. Objective: To explore the influence of B7-H4 polymorphism on the prevalence of diabetes mellitus (DM) and dyslipidemia in psoriasis. Methods: In this single-center cross-sectional study, we recruited 265 psoriatic patients receiving methotrexate (MTX) treatment. Thirteen single-nucleotide polymorphisms (SNPs) in B7-H4 were genotyped. Serum levels of total cholesterol (TC), triglycerides (TG), lipoprotein (a) (LP(a)), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein (LDL), apolipoprotein A1 (ApoA1), and apolipoprotein B (ApoB) were measured at baseline and week 12. Results: The GG genotype carriers of rs12025144 in B7-H4 had a higher prevalence of DM (57.14% vs. 17.71% vs. 18.67%, p = 0.0018), and had a poorer response to MTX in diabetic patients (p < 0.05), compared with AA or AG genotype carriers. The AG genotype of rs2066398 was associated with higher levels of pro-atherogenic lipids. MTX significantly downregulated the level of anti-atherogenic lipid ApoA1 in AA genotype carriers of rs2066398. Conclusions: The genotypes rs12025144 and rs2066398 in B7-H4 were correlated with a higher prevalence of DM and dyslipidemia in psoriasis, respectively.
Association between Peri-Implant Soft Tissue Health and Different Prosthetic Emergence Angles in Esthetic Areas: Digital Evaluation after 3 Years’ Function
Authors: Lops Diego , Romeo Eugenio , Calza Stefano , Palazzolo Antonino , Viviani Lorenzo , Salgarello Stefano , Buffoli Barbara , Mensi Magda
Published: October 2022

Background: The aim of the present retrospective study was to assess peri-implant soft tissue health for implants restored with different prosthetic emergence profile angles. Methods: Patients were treated with implants supporting fixed dentures and were followed for 3 years. Buccal emergence angle (EA) measured at 3 years of follow-up visits (t1) were calculated for two different groups: Group 1 (153 implants) for restorations with angle between implant axis and prosthetic emergence angle from ≥30°, and Group 2 (67 implants) for those with angle ≤30°, respectively. Image J software was used for the measurements. Moreover, peri-implant soft tissue parameters such as pocket probing depth (PPD), plaque index (PI) and gingival index (GI) were assessed, respectively. Results: A total of 57 patients were included in the analysis and a total of 220 implants were examined. Mean (±SD) EA in Groups 1 and 2 was 46.4 ± 12.2 and 24.5 ± 4.7 degrees, respectively. After 3 years of follow-up, a PPD difference of 0.062 mm (CI95% −0.041 mm; 0.164 mm) was calculated between the two groups and was not statistically significant (p = 0.238). Similar results were found for PI (OR = 0.78, CI95% 0.31; 1.98, p = 0.599). Furthermore, GI scores of 2 and 3 were found for nine implants (5.9%) in Group 1, and for five implants in Group 2 (7.5%). A non-significant difference (p = 0.76) was found. Conclusions: Peri-implant soft-tissue health does not seem to be influenced by EA itself, when a proper emergence profile is provided for implant-supported reconstructions in anterior areas.
Repetitive Electroencephalography as Biomarker for the Prediction of Survival in Patients with Post-Hypoxic Encephalopathy
Authors: Willems Laurent M. , Rosenow Felix , Knake Susanne , Beuchat Isabelle , Siebenbrodt Kai , Strüber Michael , Schieffer Bernhard , Karatolios Konstantinos , Strzelczyk Adam
Published: October 2022

Predicting survival in patients with post-hypoxic encephalopathy (HE) after cardiopulmonary resuscitation is a challenging aspect of modern neurocritical care. Here, continuous electroencephalography (cEEG) has been established as the gold standard for neurophysiological outcome prediction. Unfortunately, cEEG is not comprehensively available, especially in rural regions and developing countries. The objective of this monocentric study was to investigate the predictive properties of repetitive EEGs (rEEGs) with respect to 12-month survival based on data for 199 adult patients with HE, using log-rank and multivariate Cox regression analysis (MCRA). A total number of 59 patients (29.6%) received more than one EEG during the first 14 days of acute neurocritical care. These patients were analyzed for the presence of and changes in specific EEG patterns that have been shown to be associated with favorable or poor outcomes in HE. Based on MCRA, an initially normal amplitude with secondary low-voltage EEG remained as the only significant predictor for an unfavorable outcome, whereas all other relevant parameters identified by univariate analysis remained non-significant in the model. In conclusion, rEEG during early neurocritical care may help to assess the prognosis of HE patients if cEEG is not available.
Sex-Related Disparities in the Prevalence of Depression among Patients Hospitalized with Type 2 Diabetes Mellitus in Spain, 2011–2020
Authors: Lopez-de-Andres Ana , Jimenez-Garcia Rodrigo , de Miguel-Díez Javier , Hernández-Barrera Valentin , del Barrio Jose Luis , Carabantes-Alarcon David , Zamorano-Leon Jose J. , Noriega Concepcion
Published: October 2022

(1) Background: Recent reports suggest a decrease in the prevalence of depression among people with diabetes and important sex-differences in the association between these conditions, however data from Spain is sparse. We aim to assess trends in the prevalence of depression and in-hospital outcomes among patients with type 2 diabetes (T2DM) hospitalized (2011–2020) identifying sex-differences. (2) Methods: Using the Spanish national hospital discharge database we analysed the prevalence of depression globally, by sex, and according to the conditions included in the Charlson comorbidity index (CCI). We tested factors associated with the presence of depression and with in-hospital mortality (IHM). Time trends in the prevalence of depression and variables independently associated with IHM were analyzed using multivariable logistic regression. (3) Results: From 2011 to 2020, we identified 5,971,917 hospitalizations of patients with T2DM (5.7% involved depression). The prevalence of depression decreased significantly between 2011 and 2020. The adjusted prevalence of depression was 3.32-fold higher in women than in men (OR 3.32; 95%CI 3.3–3.35). The highest prevalence of depression among men and women with T2DM was found among those who also had a diagnosis of obesity, liver disease, and COPD. Older age, higher CCI, pneumonia, and having been hospitalized in 2020 increased the risk of IHM in patients with T2DM and depression. Obesity was a protective factor for IHM in both sexes, with no differences detected for IHM between men and women. Among patients hospitalized with T2DM, concomitant depression was associated with lower IHM than among patients without depression (depression paradox). (4) Conclusions: The prevalence of depression decreased over time in both sexes. The prevalence of depression was over three-fold higher in women. Female sex and depression were not associated with higher IHM. Based on our results we recommend that clinicians screen regularly for depression in patients with T2DM, particularly women, younger patients, and those with multiple comorbidities.
Prognostic Utility of the Modified Glasgow Prognostic Score in Urothelial Carcinoma: Outcomes from a Pooled Analysis
Authors: Tan Daqing , Li Jinze , Lin Tianhai , Tan Ping , Zhang Jiapeng , Xiong Qiao , Jiang Jinjiang , Li Yifan , Zhang Peng , Wei Qiang
Published: October 2022

Background: Many studies explored the prognostic value of the modified Glasgow Prognostic Score (mGPS) in urothelial carcinoma (UC), but the results are controversial. This study aimed to quantify the relationship between pretreatment mGPS and survival in patients with UC. Methods: A systematic literature search was conducted using Embase, PubMed, and Web of Science to identify eligible studies published before August 2022. Pooled hazard ratios (HRs) with 95% confidence intervals (CIs) were used to assess the association between pretreatment mGPS and the prognosis of UC. Results: Thirteen eligible studies involving 12,524 patients were included. A high mGPS was significantly associated with poor overall survival (mGPS 1/0: HR = 1.33, 95% CI 1.12–1.58, p = 0.001; mGPS 2/0: HR = 2.02, 95% CI 1.43–2.84, p < 0.0001), progression-free survival (mGPS 1/0: HR = 1.26, 95% CI 1.03–1.53, p = 0.021; mGPS 2/0: HR = 1.76, 95% CI 1.12–2.77, p = 0.013), recurrence-free survival (mGPS 1/0: HR = 1.36, 95% CI 1.18–1.56, p < 0.0001; mGPS 2/0: HR = 1.70, 95% CI 1.44–2.000, p < 0.0001), and cancer-specific survival (mGPS 2/0: HR = 1.81, 95% CI 1.30–2.52, p < 0.0001). A subgroup analysis of OS also yielded similar results. Conclusions: Evidence suggests that high pretreatment mGPS in UC is closely related to poor survival. Pre-treatment mGPS is a powerful independent prognostic factor in patients with UC.
Discovering Breast Cancer Biomarkers Candidates through mRNA Expression Analysis Based on The Cancer Genome Atlas Database
Authors: Kim Dong Hyeok , Lee Kyung Eun
Published: October 2022

Background: Research on the discovery of tumor biomarkers based on big data analysis is actively being conducted. This study aimed to secure foundational data for identifying new biomarkers of breast cancer via breast cancer datasets in The Cancer Genome Atlas (TCGA). Methods: The mRNA profiles of 526 breast cancer and 60 adjacent non-cancerous breast tissues collected from TCGA datasets were analyzed via MultiExperiment Viewer and GraphPad Prism. Diagnostic performance was analyzed by identifying the pathological grades of the selected differentially expressed (DE) mRNAs and the expression patterns of molecular subtypes. Results: Via DE mRNA profile analysis, we selected 14 mRNAs with downregulated expression (HADH, CPN2, ADAM33, TDRD10, SNF1LK2, HBA2, KCNIP2, EPB42, PYGM, CEP68, ING3, EMCN, SYF2, and DTWD1) and six mRNAs with upregulated expression (ZNF8, TOMM40, EVPL, EPN3, AP1M2, and SPINT2) in breast cancer tissues compared to that in non-cancerous tissues (p < 0.001). Conclusions: In total, 20 DE mRNAs had an area under cover of 0.9 or higher, demonstrating excellent diagnostic performance in breast cancer. Therefore, the results of this study will provide foundational data for planning preliminary studies to identify new tumor biomarkers.
Does Adoption of Climate Change Adaptation Strategy Improve Food Security? A Case of Rice Farmers in Ogun State, Nigeria
Authors: Ojo Temitope Oluwaseun , Ogundeji Abiodun A. , Emenike Chijioke U.
Published: October 2022

The southwestern part of Nigeria, particularly Ogun State, is more vulnerable to the vagaries of climate change due to the high dependence on rain-fed agriculture and limited capacities to respond to climate change. In this study, factors influencing climate change adaptation strategies and its impacts on household food security of smallholder rice farmers in Ogun State were estimated. A multistage sampling technique was employed to select 120 smallholder rice farmers in the study area. The factors influencing the adoption of climate change adaptation practices and their impacts on household food security among smallholder rice farmers in Ogun State were examined using a probit model and an endogenous switching probit model (ESPM). According to the results of household dietary diversity score (HDDS), adopters of climate change adaptation techniques have higher levels of food security than non-adopters. The outcome of the ESPM shows that access to market information, access to extension agents, gender, off-farm income, and membership in cooperatives all contribute to the variations in food security experienced by both adopters and non-adopters of climate change adaptation strategies. A unit increase in adoption of climate change adaptation measures will increase household food security by about 3 units while decreasing severity in food insecurity by about 3.2 units. Therefore, it is recommended that policies that would support smallholder farmers’ decisions to embrace measures for coping with climate change should be encouraged in order to stimulate their adaptive capacity. Additionally, in order to secure the inclusive sustainability of the agricultural sector, stakeholders and NGOs must collaborate with each other to enhance the circumstances under which farmers may receive climate change information, timely agricultural loans, and policy incentives.
Assessing the Potential of Vegetation Carbon Uptake from Optimal Land Management in the Greater Guangzhou Area
Authors: Sha Zongyao , Qiu Dai , Fang Husheng , Xie Yichun , Tu Jiangguang , Tan Xicheng , Li Xiaolei , Chen Jiangping
Published: October 2022

Rapid urbanization has threatened sustainable urban development in many cities across the globe, causing green space loss and vegetation cover degradation which reduce carbon sequestration. Optimal land management practices (LMPs) in an urban context are known as ways capable of promoting urban vegetation growth and contributing to carbon sequestration. Due to variations of physical, biological, and social structures in urban areas, policymakers often lack relevant information to decide and implement site-specific LMPs. Here we try to extract the areas in need of the optimal LMPs, identify location-dependent optimal LMPs, and assess how much more carbon can be captured by applying a combination of segmenting homogeneous urban environments and neighborhood-based analysis. As one of the most developed cities in China, the greater Guangzhou area (GGA) was selected as a case study. We found that the carbon uptake from the urban vegetation in GGA could be improved on average by 185 gC m−2 yr−1 in flux (or 1.3 TgC yr−1 in total) with optimal LMPs, equivalent to a ~30% increase considering the current level of 662 gC m−2 yr−1 in flux (4.4 TgC yr−1 in total). The carbon uptake potential was found to differ considerably across locations and among different ecosystem types, highlighting spatially varied priorities for implementing optimal LMPs over the space. This study reveals the usefulness of the model in assessing carbon uptake potential from optimal LMPs and emphasizes that future urban planning may consider the importance of optimal LMPs in enhancing vegetation carbon uptake in urban planning.
Short-Term Effects of Three Types of Hamstring Stretching on Length, Neurodynamic Response, and Perceived Sense of Effort—A Randomised Cross-Over Trial
Authors: López-de-Celis Carlos , Izquierdo-Nebreda Pedro , González-Rueda Vanessa , Cadellans-Arróniz Aïda , Rodríguez-Sanz Jacobo , Bueno-Gracia Elena , Pérez-Bellmunt Albert
Published: October 2022

Background: Stretching techniques for hamstring muscles have been described both to increase muscle length and to evaluate nerve mechanosensitivity. Aim: We sought to evaluate the short-term effects of three types of hamstring stretching on hamstring length and report the type of response (neural or muscular) produced by ankle dorsiflexion and perceived sense of effort in asymptomatic subjects. Methods: A randomised cross-over clinical trial was conducted. A total of 35 subjects were recruited (15 women, 20 men; mean age 24.60 ± 6.49 years). Straight leg raises (SLR), passive knee extensions (PKE), and maximal hip flexion (MHF) were performed on dominant and non-dominant limbs. In addition, the intensity of the applied force, the type and location of the response to structural differentiation, and the perceived sensation of effort were assessed. Results: All stretching techniques increased hamstring length with no differences between limbs in the time*stretch interaction (p < 0.05). The perceived sensation of effort was similar between all types of stretching except MHF between limbs (p = 0.047). The type of response was mostly musculoskeletal for MHF and the area of more neural response was the posterior knee with SLR stretch. Conclusions: All stretching techniques increased hamstring length. The highest percentage of neural responses was observed in the SLR stretching, which produced a greater increase in overall flexibility.
Prognostic Factors of In-Hospital Mortality in Patients with Acute Myocardial Infarction Complicated by Cardiogenic Shock
Authors: Sato Takanori , Saito Yuichi , Suzuki Sakuramaru , Matsumoto Tadahiro , Yamashita Daichi , Saito Kan , Wakabayashi Shinichi , Kitahara Hideki , Sano Koichi , Kobayashi Yoshio
Published: October 2022

Among patients with acute myocardial infarction (MI) complicated by cardiogenic shock (CS), in-hospital mortality remains high. In the present study, we aimed to identify factors associated with clinical outcomes of acute MI patients with CS in a contemporary setting. A total of 1102 patients with acute MI undergoing primary percutaneous coronary intervention were included, among whom 196 (17.8%) were complicated by CS. The primary outcome was all-cause death during hospitalization, and factors associated with in-hospital mortality were explored in patients with acute MI and CS. Of the 196 patients with acute MI complicated by CS, 77 (39.3%) died during hospitalization. The rates of non-ST-segment elevation MI (NSTEMI) (33.8% vs. 19.3%, p = 0.02) and culprit lesion in the left main or left anterior descending coronary artery (68.8% vs. 47.9%, p = 0.004) were higher, while left ventricular ejection fraction (LVEF) was lower (24.4 ± 11.7% vs. 39.7 ± 13.8%, p < 0.001) in non-survivors than in survivors. Multivariable analysis identified NSTEMI presentation and lower LVEF as independent predictors of in-hospital death. In conclusion, NSTEMI and low LVEF were identified as factors associated with higher in-hospital mortality. The identification of even higher-risk subsets and targeted therapeutic strategies may be warranted to improve survival of patients with acute MI and CS.
Nystatin Effectiveness in Oral Candidiasis Treatment: A Systematic Review & Meta-Analysis of Clinical Trials
Authors: Rai Anamika , Misra Satya Ranjan , Panda Saurav , Sokolowski Grzegorz , Mishra Lora , Das Rupsa , Lapinska Barbara
Published: October 2022

Oral candidiasis is the most common opportunistic fungal infection caused by commensal Candida species. Since there are various local and systemic predisposing factors for the disease, the treatment also varies from topical to systemic antifungal agents. Nystatin is a common antifungal agent used topically. The aim of this systematic review was to evaluate and compare the efficacy of different antifungal agents and the safety of nystatin in the treatment of oral candidiasis. Three electronic databases were searched for randomized controlled trials comparing nystatin with other anti-fungal therapies or placebo. Clinical and/or mycological cure was the outcome evaluation. A meta-analysis and descriptive study on the efficacy, treatment protocols, and safety of nystatin was also conducted. The meta-analysis included five studies, which compared the efficacy of nystatin suspensions with photodynamic therapy. A significant difference in the colony-forming units per milliliters (CFU/mL) of Candida species was observed at 60 days intervals for both palatal mucosa and denture surfaces, with both groups favoring nystatin with low heterogeneity at a 95% confidence interval. Nystatin and photodynamic therapy were found to be equally effective for the clinical remission of denture stomatitis as well as a significant reduction of CFU/mL of Candida species from dentures and palatal surfaces of the patients.
Proteomic Identification of Markers of Membrane Repair, Regeneration and Fibrosis in the Aged and Dystrophic Diaphragm
Authors: Gargan Stephen , Dowling Paul , Zweyer Margit , Henry Michael , Meleady Paula , Swandulla Dieter , Ohlendieck Kay
Published: October 2022

Deficiency in the membrane cytoskeletal protein dystrophin is the underlying cause of the progressive muscle wasting disease named Duchenne muscular dystrophy. In order to detect novel disease marker candidates and confirm the complexity of the pathobiochemical signature of dystrophinopathy, mass spectrometric screening approaches represent ideal tools for comprehensive biomarker discovery studies. In this report, we describe the comparative proteomic analysis of young versus aged diaphragm muscles from wild type versus the dystrophic mdx-4cv mouse model of X-linked muscular dystrophy. The survey confirmed the drastic reduction of the dystrophin-glycoprotein complex in the mdx-4cv diaphragm muscle and concomitant age-dependent changes in key markers of muscular dystrophy, including proteins involved in cytoskeletal organization, metabolite transportation, the cellular stress response and excitation-contraction coupling. Importantly, proteomic markers of the regulation of membrane repair, tissue regeneration and reactive myofibrosis were detected by mass spectrometry and changes in key proteins were confirmed by immunoblotting. Potential disease marker candidates include various isoforms of annexin, the matricellular protein periostin and a large number of collagens. Alterations in these proteoforms can be useful to evaluate adaptive, compensatory and pathobiochemical changes in the intracellular cytoskeleton, myofiber membrane integrity and the extracellular matrix in dystrophin-deficient skeletal muscle tissues.
Aphasic Syndromes in Cerebral Venous and Dural Sinuses Thrombosis—A Review of the Literature
Authors: Munteanu Georgiana , Motoc Andrei Gheorghe Marius , Dan Traian Flavius , Gogu Anca Elena , Jianu Dragos Catalin
Published: October 2022

Aphasia is an acquired central disorder of language that affects a person’s ability to understand and/or produce spoken and written language, caused by lesions situated usually in the dominant (left) cerebral hemisphere. On one hand aphasia has a prevalence of 25–30% in acute ischemic stroke, especially in arterial infarcts. On the other hand, cerebral venous and dural sinuses thrombosis (CVT) remains a less common and underdiagnosed cause of ischemic stroke (0.5–1% of all strokes). Aphasia has been observed in almost 20% of patients who suffered CVT. The presence of aphasia is considered a negative predictive factor in patients with stroke, severe language disorders corresponding to arduous recovery. Taking into consideration data from the literature, aphasia is also considered a predictive factor for patients with CVT; its absences, together with the absence of worsening after admission, are determinants of complete recovery after CVT. This review has as the principal role of gathering current information from the literature (PubMed database 2012–2022) regarding the clinical features of aphasic syndromes and its incidence in patients with CVT. The main conclusion of this review was that aphasic syndromes are not usually the consequence of isolated thrombosis of dural sinuses or cerebral veins thrombosis. The most frequent form of CVT that determines aphasia is represented by the left transverse sinus thrombosis associated with a posterior left temporal lesion (due to left temporal cortical veins thrombosis), followed by the superior sagittal sinus thrombosis associated with a left frontal lesion (due to left frontal cortical veins thrombosis). Only a few cases are presenting isolated cortical veins thrombosis and left thalamus lesions due to deep cerebral vein thrombosis. We also concluded that the most important demographic factor was the gender of the patients, women being more affected than men, due to their postpartum condition.
Friction and Wear Reduction of Tungsten Carbide and Titanium Alloy Contacts via Graphene Nanolubricant
Authors: Goralka Chris , Bridges Jake , Jahan Muhammad , Sidebottom Mark , Cameron Timothy , Lu Yan , Ye Zhijiang
Published: October 2022

The tribological behavior of graphene as an additive in a water-based nanofluid lubricant was investigated using pin-on-disk tests on titanium alloy (Ti-6Al-4V) and cemented tungsten carbide (WC–Co) contacts. The effect of graphene concentration and surface roughness was investigated. A non-monotonic trend of friction and wear with increasing concentration was observed. An optimal graphene concentration of 0.10 wt.% was found to provide the lowest friction and wear at different surface roughnesses, with the friction, specific wear rate of the sample surface, and tip wear reduced by 29%, 37%, and 95%, respectively. The friction reduction and anti-wear performance of the nanofluids increased as the sample surface roughness increased. The non-monotonic friction and wear trends can be explained by the agglomeration of graphene around the contact zone, where too small a graphene concentration does not provide enough lubrication and too high a concentration prevents sliding owing to a large amount of agglomeration particles at the contact. The superior friction and wear performance of the graphene nanolubricants demonstrate its potential in minimum quantity lubrication (MQL) and other applications.
Evaluation of the Safety and Efficacy of Ormeloxifene, a Selective Estrogen Receptor Modulator and Medroxyprogesterone Acetate in Women with Non-Structural Abnormal Uterine Bleeding: A Randomized Clinical Trial
Authors: Mir Suhail Ahmad , Ara Rifat , Amin Fiza , Malik Anjum , Hamid Laraibah , Ali Tabasum , Bader Ghulam Nabi , Wani Shahid Ud Din , Almuqbil Mansour , Alshehri Sultan , Alshehri Abdulhakeem M. , Shakeel Faiyaz
Published: October 2022

Background and objectives: Abnormal uterine bleeding is a significant clinical and gynaecological concern that necessitates its safe and effective treatment. The present study aims to compare the cost-effectiveness, safety, efficacy, and health-related quality of life of ormeloxifene with medroxyprogesterone acetate in women with non-structural abnormal uterine bleeding. Materials and Methods: A prospective, randomized, single-blinded clinical trial of 367 patients was carried out at a tertiary care hospital for a period of one year from 5 January 2019 to 4 January 2020. Patients were randomized into two groups for administering ormeloxifene and medroxyprogesterone acetate for a 3-month treatment duration and were evaluated by laboratorial investigations like anaemic status, bleeding duration, endometrial thickness, pictorial blood loss assessment chart (PBLAC) score, and patient’s medical and medication history. Health-related quality of life was assessed using short form survey-36 (SF-36) questionnaire scale. Cost-effectiveness was determined on the basis of the three-month treatment regimen. Results: The mean duration of bleeding reduced from 16.88 ± 6.46 to 7.76 ± 1.55 in the ormeloxifene group and from 15.91 ± 5.04 to 8.7 ± 1.91 (p < 0.001) in the medroxyprogesterone acetate. Similarly, mean haemoglobin increased from 8.56 ± 0.77 to 10.1 ± 0.087 g/dL and from 8.60 ±0.97 to 9.551 ± 0.90 g/dL (p < 0.001), and endometrial thickness showed a reduction from 8.52 ± 1.61 mm to 6.92 ± 1.68 mm and from 8.40 ± 2.09 mm to 7.85 ± 2.0 mm (p < 0.001) in the ormeloxifene and medroxyprogesterone acetate groups, respectively. PBLAC score reduced from 289.92 ± 42.39 to 128.11 ± 33.10 and from 287.38 ± 40.94 to 123.5 ± 29.57 (p < 0.001) in these groups, respectively. Health-related quality of life improved in the ormeloxifene group more than the medroxyprogesterone group, which was evidenced by SF-36 scale parameters (physical function, energy/fatigue and pain) that changed from 24.39, 12.99, 6.25 to 28.95, 18, 9 and from 25.41, 13.6, 7.1 to 27.02, 16, 8.3 in the ormeloxifene and medroxyprogesterone acetate groups, respectively. Conclusions: The study concludes that both medroxyprogesterone acetate and ormeloxifene are safe and efficacious in controlling abnormal uterine bleeding, but ormeloxifene was the better of the two in terms of cost effectiveness, reduction in pictorial blood loss assessment score, endometrial thickness, bleeding duration (days), increase in haemoglobin concentration (g/dL) and improvement in the quality of life.
Prognostic Value of Plasma Presepsin and Pneumonia Severity Index in Patients with Community-Acquired Pneumonia in the Emergency Department
Authors: Lee Kyeong-Ryong , Hong Dae-Young , Paik Jin-Hui , Jung Hyun-Min
Published: October 2022

Background and Objectives: Presepsin (PSS) is an independent predictor for estimating disease severity and prognosis in septic patients. Few studies have reported the associations between plasma PSS and the severity and prognosis in patients with community-acquired pneumonia (CAP). We investigated whether a high plasma PSS level was associated with 30-day mortality in CAP patients. Materials and Methods: This retrospective single-center study was conducted in an emergency department. The PSS level was measured in 211 adult CAP patients admitted to the hospital and followed for up to 30 days. We recorded the pneumonia severity index (PSI) and the CURB-65 score. The primary outcome was death from any cause within 30 days. Results: The plasma PSS levels were significantly elevated in the high-risk group (PSI > 130) compared with the low- (PSI < 91) or moderate-risk groups (PSI 91–130). Forty-four patients (20.9%) died within 30 days of admission. Non-survivors had significantly higher plasma PSS levels than survivors among CAP patients: 1083 (697–1736) pg/mL vs. 385 (245–554) pg/mL (p < 0.001). The area under the curve (AUC) to predict 30-day mortality was highest for PSS (0.867), followed by procalcitonin (0.728) and lactate (0.616). The cutoff level of plasma PSS for 30-day mortality was >754 pg/mL. The combination of PSI and plasma PSS level improved the predictive ability for 30-day mortality (AUC = 0.892). Cox regression analysis showed that higher PSS levels (>754 pg/mL) and higher PSI (>126) were associated with 30-day mortality in CAP patients (hazard ratios of 19.472 and 6.375, respectively). Conclusion: Elevated plasma PSS is associated with severity and 30-day mortality in hospitalized CAP patients. Combining plasma PSS level and PSI could significantly improve the predictive ability of PSS for 30-day mortality.
Concentration of Polyphenolic Antioxidants in Apple Juice and Extract Using Ultrafiltration
Authors: Dushkova Mariya , Mihalev Kiril , Dinchev Angel , Vasilev Kiril , Georgiev Diyan , Terziyska Margarita
Published: October 2022

The aim of the present work was to study the potential of ultrafiltration with three polyacrylonitrile membranes (1, 10, and 25 kDa) to concentrate polyphenolic antioxidants in apple juice and extract. The permeate flux, total polyphenols, polyphenolic profile, phenolic acid content, and total antioxidant capacity were determined using the FRAP and DPPH tests, the content of water-soluble proteins during ultrafiltration was established, and the concentration factors and rejections were determined. The permeate flux decreased by increasing the volume reduction ratio and decreasing the molecular weight cut-off of the membranes. The concentration factor and rejection of polyphenolics increased with the increase in the volume reduction ratio (VRR) for all membranes and both liquids. The concentration and rejection effectiveness of the 1 kDa membrane was higher than those observed for 10 and 25 kDa during the ultrafiltration of the apple extract, while these values were comparable for 1 and 10 kDa during the ultrafiltration of the apple juice. The concentration factors and rejections of total polyphenols were higher in the extract than in the juice. Chlorogenic acid was the main compound in the polyphenol profile of apple juice. The total content of phenolic acids, determined by using HPLC, increased by 15–20% as a result of the membrane concentration, but the separation process did not significantly change the ratio between the individual compounds.
Simulation Study of Ultrasonic Elliptical Vibration Cutting of TiC Particle-Reinforced Titanium Matrix Composites
Authors: Huan Haixiang , Xu Wenqiang , Zhao Biao , Zhang Ke , Pu Jianfei , Zhu Chilei
Published: October 2022

In order to investigate the characteristics of elliptical ultrasonic vibration cutting of TiC particle-reinforced titanium matrix composites, a two-dimensional thermodynamic coupled finite element cutting model was established based on the Johnson-Cook intrinsic structure model using ABAQUS simulation software, and the changes in cutting force, cutting temperature, machined surface shape, and particle fragmentation were obtained under the traditional cutting method and ultrasonic elliptical vibration cutting method. The results show that under the same process parameters, ultrasonic elliptical vibration cutting is better than conventional cutting in terms of surface profile; the stress direction tends to be horizontal during cutting and the TiC particles are mainly removed by cutting off. The average cutting force is significantly lower than conventional cutting, with a maximum reduction of 60%. The cutting temperature is also reduced, with a reduction of approximately 17.6%.
Optimization of Thermomechanical Processing under Double-Pass Hot Compression Tests of a High Nb and N-Bearing Austenitic Stainless-Steel Biomaterial Using Artificial Neural Networks
Authors: Sulzbach Gláucia Adriane de S. , Rodrigues Maria Verônica G. , Rodrigues Samuel F. , Lima Marcos Natan da S. , Loureiro Rodrigo de C. Paes , Sá Denis Fabrício S. de , Aranas Clodualdo , Macedo Glaucia Maria E. , Siciliano Fulvio , Abreu Hamilton F. Gomes de , Reis Gedeon S. , Silva Eden S.
Published: October 2022

Physical simulation is a useful tool for examining the events that occur during the multiple stages of thermomechanical processing, since it requires no industrial equipment. Instead, it involves hot deformation testing in the laboratory, similar to industrial-scale processes, such as controlled hot rolling and forging, but under different conditions of friction and heat transfer. Our purpose in this work was to develop an artificial neural network (ANN) to optimize the thermomechanical behavior of stainless-steel biomaterial in a double-pass hot compression test, adapted to the Arrhenius–Avrami constitutive model. The method consists of calculating the static softening fraction (Xs) and mean recrystallized grain size (ds), implementing an ANN based on data obtained from hot compression tests, using a vacuum chamber in a DIL 805A/D quenching dilatometer at temperatures of 1000, 1050, 1100 and 1200 °C, in passes (ε1 = ε2) of 0.15 and 0.30, a strain rate of 1.0 s−1 and time between passes (tp) of 1, 10, 100, 400, 800 and 1000 s. The constitutive analysis and the experimental and ANN-simulated results were in good agreement, indicating that ASTM F-1586 austenitic stainless steel used as a biomaterial undergoes up to Xs = 40% of softening due solely to static recovery (SRV) in less than 1.0 s interval between passes (tp), followed by metadynamic recrystallization (MDRX) at strains greater than 0.30. At T > 1050 °C, the behavior of the softening curves Xs vs. tp showed the formation of plateaus for long times between passes (tp), delaying the softening kinetics and modifying the profile of the curves produced by the moderate stacking fault energy, γsfe = 69 mJ/m2 and the strain-induced interaction between recrystallization and precipitation (Z-phase). Thus, the use of this ANN allows one to optimize the ideal thermomechanical parameters for distribution and refinement of grains with better mechanical properties.
Comprehensive Weldability Criterion for Magnetic Pulse Welding of Dissimilar Materials
Authors: Kapil Angshuman , Mastanaiah P. , Sharma Abhay
Published: October 2022

Despite its exceptional ability to join dissimilar materials and environmental friendliness, several challenges must be addressed in magnetic pulse welding (MPW). The conventional weldability criterion (i.e., minimum impact velocity) is analytically calculated as a function of material properties without considering the geometry of electromagnetic coil, electrical and physical parameters, making the minimum impact velocity a necessary but not sufficient condition for a sound MPW joint. A new weldability criterion, namely effective impact velocity, is proposed, which overcomes the conventional weldability criterion’s limitations. The effective impact velocity can be inversely modelled to identify shop-floor relevant process parameters and it eliminates the need to fabricate several coils in the process and product proving stages. The proposed approach is demonstrated by a case study on tubular welding of Aluminium and SS304. The weld’s soundness produced with computed process parameters was corroborated by experimental observations on lap shear tests, hardness measurements, optical and scanning electron microscopy, and surface energy dispersive spectroscopy mapping. This investigation is expected to pave the way for developing the process window for MPW of several material combinations, with high cost and time savings.