ARTIFICIAL FIELD INOCULATION OFBREAD WHEAT ADVANCED LINES WITH TILLETIAINDICA, CAUSAL AGENT OF KARNAL BUNT
Authors:   Inifap, Campo Experimental Norman E. BORLAUG, Apdo. Postal 155, km 12 Norman E. Borlaug, Entre 800 y 900, Valle del Yaqui, Cd. Obregon, Sonora, Mexico CP 85000., Fuentes-Davila Guillermo , Prakash-Singh Ravi ,   CimmytInt.,km 45 Carret. Mexico-Veracruz, El Batan, Texcoco, Edo. de Mexico CP 56130., Alejandra Rosas-Jauregui Ivon ,   Inifap, Campo Experimental Norman E. BORLAUG, Apdo. Postal 155, km 12 Norman E. Borlaug, Entre 800 y 900, Valle del Yaqui, Cd. Obregon, Sonora, Mexico CP 85000., Antonio Ayon-Ibarra Carlos ,   Inifap, Campo Experimental Norman E. BORLAUG, Apdo. Postal 155, km 12 Norman E. Borlaug, Entre 800 y 900, Valle del Yaqui, Cd. Obregon, Sonora, Mexico CP 85000., Monserrat Torres-Cruz Maria ,   Inifap, Campo Experimental Norman E. BORLAUG, Apdo. Postal 155, km 12 Norman E. Borlaug, Entre 800 y 900, Valle del Yaqui, Cd. Obregon, Sonora, Mexico CP 85000., Felix-Valencia Pedro ,   Inifap, Campo Experimental Norman E. BORLAUG, Apdo. Postal 155, km 12 Norman E. Borlaug, Entre 800 y 900, Valle del Yaqui, Cd. Obregon, Sonora, Mexico CP 85000., Luis Felix-Fuentes Jose ,   Inifap, Campo Experimental Norman E. BORLAUG, Apdo. Postal 155, km 12 Norman E. Borlaug, Entre 800 y 900, Valle del Yaqui, Cd. Obregon, Sonora, Mexico CP 85000.
Published: January 2022

The reaction to Tilletiaindica of one thousand and ninety twobread wheat advanced lines were evaluated in the field during the crop season 2016-2017. Sowing in beds with two rows was carried out on November 11 and 24, 2016, using 8 g of seed. Five spikes per line were inoculated by injection with 1 mL of an allantoidsporidial suspension (10,000/mL) during the boot stage, and at maturity the percentage of infection was determined by counting healthy and infected grains. The range of infection in the first date was 0-88.83 with a mean of 31.81%, while in the second date it was 0-82.65% with a mean of 24.44%.The range of infection of the two dates was 0.46-83.71% with a mean of 28.12%.Sixteenlines showed a percentage of infection equal or below 5.0% in both dates, and out of those lines, the following five showed less than 2.5%: two sister lines of MUNAL#1/FRANCOLIN#1*2/3/ATTILA*2/PBW65//MURGA(CMSS12Y00701T-099TOPM-099Y-099M-0SY-13M-0WGY), MUNAL#1/FRANCOLIN#1*2/3/ATTILA*2/PBW65//MURGA (CMSS12Y00701T-099TOPM-099Y-099M-0SY-17M-0WGY), BAJ#1/3/KIRITATI//ATTILA*2/PASTOR*2/4/MUTUS*2/TECUE#1, VILLAJUAREZF2009/6/ATTILA/3*BCN//BAV92/3/PASTOR/4/TACUPETOF2001*2/BRAMBLING/5/PAURAQ, and KACHU/BECARD//WBLL1*2/BRAMBLING/4/FRET2/TUKURU//FRET2/3/MUNAL#1. Lines with the highest percentage of infection were: BABAX/LR42//BABAX*2/3/KUKUNA/4/CROSBILL#1/5/BECARD/6/KSW/SAUAL//SAUAL/7/BABAX/LR42//BABAX*2/3/KUKUNA/4/CROSBILL#1/5/BECARD with 88.83 in the first date,MUU/KBIRD//2*KACHU/KIRITATIwith 84.77 and 82.65%in the first and second date, respectively, and TACUPETOF2001*2/BRAMBLING//WBLL1*2/BRAMBLING/6/WBLL1*2/KURUKU*2/5/REH/HARE//2*BCN/3/CROC_1/AE.SQUARROSA(213)//PGO/4/HUITES/7/BAV92//IRENA/KAUZ/3/HUITES/4/2*ROLF07 with 81.67% in the first date. The average of the three highest levels of infection of the susceptible checkwas99.7%.