bluuebunny/crossref_metadata_2025 huggingface dataset:
Colistin, Meropenem–Vaborbactam, Imipenem–Relebactam, and Eravacycline Testing in Carbapenem-Resistant Gram-Negative Rods: A Comparative Evaluation of Broth Microdilution, Gradient Test, and VITEK 2
Authors: Forstner Patrick , Fuchs Lisa , Luxner Josefa , Grisold Andrea , Steinmetz Ivo , Dichtl Karl
Published: November 2024

Objectives. This study aimed to evaluate and compare the performance of different assays for antimicrobial susceptibility testing (AST) and minimum inhibitory concentration (MIC) determination for reserve antibiotics in carbapenem-resistant Enterobacterales (CREs), Pseudomonas aeruginosa (CRPAs), and Acinetobacter baumannii (CRABs). Methods. An analysis was conducted on 100 consecutive isolates: 50 CREs, 35 CRPAs, and 15 CRABs. Sensititre broth microdilution was used as a reference standard to evaluate the performance of VITEK 2 card AST-XN24 (bioMérieux), the respective gradient tests (bioMérieux), and UMIC colistin broth microdilution test strips (Bruker Daltonics). Errors, essential agreement (EA), and categorical agreement of MICs for colistin (COL), meropenem–vaborbactam (MVB), imipenem–relebactam (IRL), and eravacycline (ERV) were assessed. Results. The agreement between both of the COL broth microdilution (BMD) methods was perfect (100/100). The gradient test and VITEK 2 analysis yielded comparable EA rates (92/100 and 72/79, respectively), with the latter not registering any very major errors (VMEs). The MVB gradient test achieved EA in 66 of 85 isolates and VITEK 2 in 70/85. For IRL, EA was reached in 69 and 64 of 85 cases by gradient test and VITEK 2 analysis, respectively. The ERV gradient test yielded false results in nearly all (12/15) CRABs but achieved EA in 46 of 50 CREs. The VITEK system recorded EA for ERV in 60 of 65 isolates. Conclusions. We observed substantial variability in the measured MICs between BMD and the alternative methods. In only a few constellations, VITEK 2 or gradient tests could substitute the reference method. BMD is the method of choice for COL analysis, with VITEK 2 representing an alternative method for CRPA testing. Alternative methods for MVB did not provide reliable results, except for Enterobacterales, when tested with the gradient test. However, resistant results need to be confirmed by BMD. Only BMD can be used for IRL MIC determination. VITEK 2 was mostly accurate in measuring ERV MICs, while the corresponding gradient test yielded reliable results exclusively in CREs. It is essential that laboratories are aware of which testing method provides reliable results for each combination of microorganisms and reserve antibiotics.
Deformation and Fracture Mechanisms of Thick Hard Roofs in Upward Mining Coalfaces: A Mechanical Model and Its Validation
Authors: Zhang Wei , Cao Linchao , Zhang Dongsheng , Hu Yang , Chang Jingyu , Zhang Zhenying
Published: November 2024

The safety and efficiency of underground coal mining are threatened by thick hard roofs characterized by large overhang areas, problematic spontaneous caving, and high dynamic load upon their breakage. In this study, a mechanical model of the bearing capacity of thick hard roofs in upward mining coalfaces associated with mining activities is built based on bending theories for beams with single generalized displacement and the elastic foundation beam theory. Using this method, we analyze the deformation and fracture mechanisms of a thick hard roof during upward mining. We further derive the mechanical equations of rotational angle, bending moment, shear force, and deflection of the free overhang and coal-bearing zone in the thick hard roof and an equation for calculating the limiting span. The mechanical behaviors of the thick hard roof bearing state are analyzed under different parameters. The results show that the foundation coefficient, roof thickness, and angle of upward mining have little influence on the roof bending moment but are positively correlated to the limiting span. Roof load and overhang length have a significant influence on the roof bending moment. They are negatively and positively correlated with the limiting span, respectively. Finally, a case study is performed on the Ш601 upward mining coalface in the Zhuzhuang Coal Mine. The distribution characteristics of the bending moment of the thick hard roof at different extraction stages are analyzed. At each stage, the limiting spans of the thick hard roof upon breaking were calculated as 13.18, 18.82, and 22.50 m, respectively, being close to the on-site measured periodic weighting lengths of 13.33, 19.33 m, and 22.67 m. This close fit proves the feasibility and accuracy of the developed mechanical model. The present study offers theoretical guidance for estimating the weighting length of thick hard roofs in coalfaces and for engineering technology control in similar scenarios.
Plastic Injection Molding Process Analysis: Data Integration and Modeling for Improved Production Efficiency
Authors: Hernández-Vega Jose Isidro , Reynoso-Guajardo Luis Alejandro , Gallardo-Morales Mario Carlos , Macias-Arias María Ernestina , Hernández Amadeo , de la Cruz Nain , Soto-Soto Jesús E. , Hernández-Santos Carlos
Published: November 2024

This paper presents a comprehensive analysis of the plastic injection molding process through the integration of data acquisition technologies and classification models. In collaboration with a company specializing in plastic injection, data were extracted directly from the machine during a specific period at the beginning of a shift change. These data were subjected to exploratory analysis to identify correlations between important variables, such as injection time, cycle time, and mold pressures. Additionally, classification models, including Random Forest and Logistic Regression, were constructed to predict and classify the process state based on these variables. The model results demonstrated high predictive performance, with 99.5% accuracy for Random Forest and 97% for Logistic Regression. These results provide a strong foundation for the early identification of potential problems and informed decision making to improve the efficiency of the plastic injection molding process. This study contributes to the advancement of the integration of intelligent technologies in industrial process optimization, aligned with the principles of Industry 4.0.
Microencapsulated Pomegranate Juice Reduces Metabolic Syndrome (MetS) Criteria in Wistar Rats with MetS Induced with 30% Sucrose
Authors: Guerrero-Solano José Antonio , Cancino-Díaz Juan Carlos , Jaramillo-Morales Osmar Antonio , Bautista Mirandeli , Hernández-Uribe Juan Pablo , Talamantes-Gómez José Moisés , Valadez-Vega Carmen , Belefant-Miller Helen , Betanzos-Cabrera Gabriel
Published: November 2024

Pomegranate juice possesses therapeutic properties against metabolic syndrome. Microencapsulation of pomegranate juice (MPJ) may extend its shelf life; however, further research is necessary to ascertain its comparative efficacy with fresh juice. This work evaluated the effect of MPJ in an animal model with induced metabolic syndrome (MetS). MetS was induced in Male Wistar rats by administering 30% sucrose orally for 22 weeks. Once MetS was verified in the animals, seven groups were formed as follows: control (Ctrl); MetS; MetS + Placebo; Ctrl + MPJ; MetS + MPJ1 600; MetS + MPJ2 1800; and MetS + MPJ3 2900 mg/kg. Systolic and diastolic blood pressure, glucose, triglycerides, and total HDL-c levels in the MetS + MPJ groups improved significantly (p < 0.05) vs. the MetS group at the end of the study. Microencapsulation maintained the anti-metabolic-syndrome properties of pomegranate juice and could be used to control metabolic syndrome.
A New Bivariate Survival Model: The Marshall-Olkin Bivariate Exponentiated Lomax Distribution with Modeling Bivariate Football Scoring Data
Authors: Binhimd Sulafah M. S. , Kalantan Zakiah I. , EL-Helbawy Abeer A. , AL-Dayian Gannat R. , Mahmoud Ahlam A. M. , Refaey Reda M. , Elaal Mervat K. Abd
Published: November 2024

This paper focuses on applying the Marshall-Olkin approach to generate a new bivariate distribution. The distribution is called the bivariate exponentiated Lomax distribution, and its marginal distribution is the exponentiated Lomax distribution. Numerous attributes are examined, including the joint reliability and hazard functions, the bivariate probability density function, and its marginals. The joint probability density function and joint cumulative distribution function can be stated analytically. Different contour plots of the joint probability density function and joint reliability and hazard rate functions of the bivariate exponentiated Lomax distribution are given. The unknown parameters and reliability and hazard rate functions of the bivariate exponentiated Lomax distribution are estimated using the maximum likelihood method. Also, the Bayesian technique is applied to derive the Bayes estimators and reliability and hazard rate functions of the bivariate exponentiated Lomax distribution. In addition, maximum likelihood and Bayesian two-sample prediction are considered to predict a future observation from a future sample of the bivariate exponentiated Lomax distribution. A simulation study is presented to investigate the theoretical findings derived in this paper and to evaluate the performance of the maximum likelihood and Bayes estimates and predictors. Furthermore, the real data set used in this paper comprises the scoring times from 42 American Football League matches that took place over three consecutive independent weekends in 1986. The results of utilizing the real data set approve the practicality and flexibility of the bivariate exponentiated Lomax distribution in real-world situations, and the bivariate exponentiated Lomax distribution is suitable for modeling this bivariate data set.
Plasma Proteome Alterations of Laying Hens Subjected to Heat Stress and Fed a Diet Supplemented with Pequi Oil (Caryocar brasiliense Camb.): New Insights in the Identification of Heat Stress Biomarkers
Authors: da Silva Joyce , Andrade Luane , Rodrigues Paola , Cordeiro Laís , Lima Gabrieli , Lopes Júlia , Castillo Elis , Martins Renata , Assunção Andrey , Vieira José , Busalaf Marília , Adamec Jiri , Sartori José , Padilha Pedro
Published: November 2024

Heat stress can disrupt the balance between the heat poultry release into the environment and the heat they generate. Pequi oil has antioxidant properties, which may mitigate the heat stress effects. This study aimed to investigate the response of laying hens to pequi oil supplementation under heat stress using a proteomic approach. A total of 96 Lohmann White laying hens with 26 weeks old were housed in a completely randomized design with a 2 × 2 factorial arrangement. They were housed in two climate chambers, thermal comfort temperature ± 24.04 °C with the relative humidity ± 66.35 and heat stress (HS) ± 31.26 °C with the relative humidity ± 60.62. They were fed two diets: a control diet (CON), basal diet (BD) without additives, and with Pequi oil (PO), BD + 0.6% PO. After 84 days, plasma samples were analyzed using Shotgun and LC-MS/MS. Proteins related to anti-inflammation, transport, and the immune system were differentially expressed in hens fed PO and CON under heat stress compared to those in thermoneutral environments. This helps protect against oxidative stress and may support the body’s ability to manage heat-induced damage, stabilizing protein expression under stress conditions. The ovotransferrin proteins, fibrinogen isoforms, apolipoprotein A-I, Proteasome activator subunit 4, Transthyretin, and the enzyme serine Peptidase Inhibitor_Kazal Type 5, which presented Upregulated (Up) equal to 1, present characteristics that may be crucial for enhancing the adaptive responses of hens to thermal stress, thereby increasing their tolerance and minimizing the negative effects of heat on egg production. The data presented in this manuscript provides new insights into the plasma proteome alterations of laying hens fed a diet supplemented with pequi oil during heat stress challenges.
Preferences and Expectations for Home Robot Tasks: Comparison According to Age and Household Type in Republic of Korea
Authors: Yoo Ha-Yeon , Kim Minjun
Published: November 2024

Most studies of consumer preferences and expectations for home robots focus on either older adults or single-person households (SPHs). However, with the rise in voluntary SPHs among young adults and seniors, it is critical to compare both age and household types in robot research. This study explored perceptions of home robots and willingness to use their features based on age and household type, in the context of the expanding home robot market in Republic of Korea. An online survey of 400 individuals was conducted, targeting young SPHs and multi-person households (MPHs) in their 20s and 30s as well as older SPHs and MPHs in their 50s and 60s. The survey covered four robot task categories, with 40 items derived from previous research: household chores (20 items), personal care (seven items), leisure/companion (nine items), and health (four items). The results helped predict the main target groups for each in-home robot task by identifying items that showed differences in responses between groups and interpreting these based on age, household type, and their combination. The study provides valuable data on consumer expectations, highlighting differences in responses according to both age and household type, offering insights for the robotics industry to effectively target their products.
Sodium-Selective Channelrhodopsins
Authors: Coli Ariel , Gao Shiqiang , Kaestner Lars
Published: November 2024

Channelrhodopsins (ChRs) are light-gated ion channels originally discovered in algae and are commonly used in neuroscience for controlling the electrical activity of neurons with high precision. Initially-discovered ChRs were non-selective cation channels, allowing the flow of multiple ions, such as Na+, K+, H+, and Ca2+, leading to membrane depolarization and triggering action potentials in neurons. As the field of optogenetics has evolved, ChRs with more specific ion selectivity were discovered or engineered, offering more precise optogenetic manipulation. This review highlights the natural occurrence and engineered variants of sodium-selective channelrhodopsins (NaChRs), emphasizing their importance in optogenetic applications. These tools offer enhanced specificity in Na+ ion conduction, reducing unwanted effects from other ions, and generating strong depolarizing currents. Some of the NaChRs showed nearly no desensitization upon light illumination. These characteristics make them particularly useful for experiments requiring robust depolarization or direct Na+ ion manipulation. The review further discusses the molecular structure of these channels, recent advances in their development, and potential applications, including a proposed drug delivery system using NaChR-expressing red blood cells that could be triggered to release therapeutic agents upon light activation. This review concludes with a forward-looking perspective on expanding the use of NaChRs in both basic research and clinical settings.
Nuclear–Cytoplasmic Shuttling of the Usher Syndrome 1G Protein SANS Differs from Its Paralog ANKS4B
Authors: Fritze Jacques S. , Stiehler Felizitas F. , Wolfrum Uwe
Published: November 2024

The USH1G protein SANS is a small multifunctional scaffold protein. It is involved in several different cellular processes, such as intracellular transport, in the cytoplasm, or splicing of pre-mRNA, in the cell nucleus. Here, we aimed to gain insight into the regulation of the subcellular localization and the nuclear–cytoplasmic shuttling of SANS and its paralog ANKS4B, not yet reported in the nucleus. We identified karyopherins mediating the nuclear import and export by screening the nuclear interactome of SANS. Sequence analyses predicted in silico evolutionarily conserved nuclear localization sequences (NLSs) and nuclear export sequences (NESs) in SANS, but only NESs in ANKS4B, which are suitable for karyopherin binding. Quantifying the nuclear–cytoplasmic localization of wild-type SANS and NLS/NES mutants, we experimentally confirmed in silico predicted NLS and NES functioning in the nuclear–cytoplasmic shuttling in situ in cells. The comparison of SANS and its paralog ANKS4B revealed substantial differences in the interaction with the nuclear splicing protein PRPF31 and in their nuclear localization. Finally, our results on pathogenic USH1G/SANS mutants suggest that the loss of NLSs and NESs and thereby the ability to control nuclear–cytoplasmic shuttling is disease-relevant.
A Proposed Method of Automating Data Processing for Analysing Data Produced from Eye Tracking and Galvanic Skin Response
Authors: Sáez-García Javier , Sáiz-Manzanares María Consuelo , Marticorena-Sánchez Raúl
Published: November 2024

The use of eye tracking technology, together with other physiological measurements such as psychogalvanic skin response (GSR) and electroencephalographic (EEG) recordings, provides researchers with information about users’ physiological behavioural responses during their learning process in different types of tasks. These devices produce a large volume of data. However, in order to analyse these records, researchers have to process and analyse them using complex statistical and/or machine learning techniques (supervised or unsupervised) that are usually not incorporated into the devices. The objectives of this study were (1) to propose a procedure for processing the extracted data; (2) to address the potential technical challenges and difficulties in processing logs in integrated multichannel technology; and (3) to offer solutions for automating data processing and analysis. A Notebook in Jupyter is proposed with the steps for importing and processing data, as well as for using supervised and unsupervised machine learning algorithms.
Investigation into the Rolling Process of 20CrNiMo/Incoloy 825 Composite Materials
Authors: Liu Jie , Gui Hailian , Zhang Peng , Zhang Chen , Liu Hao
Published: November 2024

This paper is focused on the rolling finite element simulation and experimental study of 20CrNiMo/Incoloy 825 composite materials. Firstly, single-pass rolling finite element simulations of the composite materials were conducted. The effects of rolling pass reduction and rolling speed on the warpage, interface strain difference, and stress of the 20CrNiMo/Incoloy 825 composite materials were evaluated, highlighting an ideal first-pass reduction of 30% and a rolling speed of 0.117 m/s. Based on these results, rolling finite element simulations under total reduction–pass conditions of 65%–3 passes, 75%–4 passes, and 85%–5 passes were conducted on 20CrNiMo/Incoloy 825 composite materials. The rolling process was found to be optimal for a total reduction of 85%–5 passes based on the ratio of the vertical compressive stress experienced by the Incoloy 825-side metal to the yield strength of Incoloy 825 at 1150 °C. Based on the results of single- and multi-pass finite element simulation experiments, microstructural observations and interface analyses were then conducted on the 20CrNiMo/Incoloy 825 composite materials after rolling. The bonding interface of the composite materials was found to be undulating, indicating good composite effects. In addition, Cr, Ni, and Fe at the interface of the composite materials exhibited a steep gradient of change, indicating trace element diffusion with a distance of 8.27 μm in the 20CrNiMo/Incoloy 825 composite materials. Finally, the interfacial bonding mechanism of the 20CrNiMo/Incoloy 825 composite materials was studied, and the results indicate that this mechanism is based on a combination of diffusion and recrystallization bonding mechanisms.
A Programmable Gate Driver Module-Based Multistage Voltage Regulation SiC MOSFET Switching Strategy
Authors: Tan Jixiang , Zhou Zhongfu , Zou Gongjie
Published: November 2024

Silicon carbide (SiC) metal-oxide semiconductor field-effect transistors (MOSFETs), as a new material, have the advantages of low drain-source resistance, high thermal conductivity, low leakage current, and high switching frequency compared with silicon (Si)-based MOSFETs. Therefore, in many industrial applications, Si MOSFETs have been replaced by SiC MOSFETs. However, as the switching speed increases exponentially, some problems are amplified, the most serious of which is the overshoot of current and voltage. The increase in voltage and current slope caused by high switching speeds inevitably leads to overshoot, oscillations, and additional losses in the circuit. This paper focusses on the actual performance of the optimised switching strategy (OSS) in circuit testing and combines the existing simulation results to verify the practicability of OSS. In this paper, the optimised switching strategy is introduced first, and then, the LTspice model of SiC MOSFET is established in detail and verifies the feasibility of the OSS through half-bridge circuit simulation. Finally, the test platform is built using a programmable gate drive module (2ASC-12A1HP). Through a 400 V/30 A double-pulse test, the practicality of the OSS is verified. The experiments show that the OSS can greatly improve the switching performance of SiC MOSFETs.
How Does the Nexus Between Digitalization and Banking Performance Drive Digital Transformation in Central and Eastern European Countries?
Authors: Manta Alina Georgiana , Bădîrcea Roxana Maria , Gherțescu Claudia , Manta Liviu Florin
Published: November 2024

The aim of this paper is to create a digitalization index for banking sectors using a set of indicators based on World Bank data for the period of 2010–2021, which will allow us to rank the sectors of Central and Eastern European countries (CEECs). The digitalization index is built based on how ready banks are for digitalization, the potential customers available for digital banking, and the level of digital infrastructure, with each of these aspects representing one pillar. Based on the calculation of the digitalization index, we emphasize that Romania is the leader, followed by Latvia and Lithuania, while Hungary and Estonia are at the opposite pole. Furthermore, we applied the fully modified ordinary least squares (FMOLS) method to measure the impact of digitalization on banking performance. This study reveals that Romania, Latvia, and Lithuania lead in digital banking transformation due to significant investments in infrastructure and customer engagement, while Hungary and Poland lag in terms of digital readiness. The results indicate that digitalization has a significant positive effect on banking performance (ROE), although countries experiencing market saturation had the potential to see a decline post-2018, necessitating further innovation to sustain growth. In the digitalization context, the results are relevant for policymakers, showing that investing more in digitalization is important and that there is a need to help people have greater access to banking services due to a lack of willingness and financial education, factors which prevent them from embracing digital changes. The results show that improving banking digitalization positively influences banking performances. This study provides an innovative and complex index for assessing banking digitalization in Central and Eastern Europe, with valuable implications for policymakers. We highlight the need to align digitalization policies with the specific level of digital development of each country in order to optimize the integration of digital technologies and enhance economic competitiveness.
Dynamic Path Planning Scheme for OHT in AMHS Based on Map Information Double Deep Q-Network
Authors: Ao Qi , Zhou Yue , Guo Wei , Wang Wenguang , Ye Ying
Published: November 2024

AMHSs (Automated Material Handling Systems) are widely used in major Fabs (semiconductor fabrication plants). The OHT in an AMHS is responsible for handling the FOUP (Front Opening Unified Pod) within the Fabs. Due to the unidirectional track, the movement path of the OHT aims to avoid congested areas caused by operations or malfunctions as much as possible, to improve the overall FOUP handling efficiency. To do so, we propose a dynamic path planning method, MI-DDQN (Map Information Double Deep Q-Network), driven by deep reinforcement learning and based on map information. Firstly, we design and establish a map information state space model based on the core elements of the OHT path planning in the AMHS. Then, we design an OHT motion simulator to simulate the position coordinate transformation of the OHT, providing real-time coordinate update data for the OHT during the algorithm training process. We design a deep reinforcement learning algorithm structure based on map information model and a convolutional neural network model structure and use the algorithm to train the network model. Finally, the designed task generation module and OHT motion simulator are used to randomly generate the starting position and task position of the OHT during the training process to enhance the richness of the data. The addition of a “fault” OHT verifies the method’s ability to plan routes in complex road conditions such as congestion that may occur at any time.
Upper and Lower Endoscopic Findings in Mesenteric Panniculitis Patients: A Case-Control Study
Authors: Schweistein Hagai , Weintraub Yoav , Hornik-Lurie Tzipi , Haskiya Hassan , Rave Adi , Glusman Bendersky Ahinoam , Issa Nidal , Naftali Timna , Gingold-Belfer Rachel
Published: November 2024

Background: The natural history and prognosis of mesenteric panniculitis (MP) are not well-described. Despite referral for colonoscopy being common for this indication, colonoscopy findings in MP patients have not been reported. Therefore, we aimed to describe upper and lower gastrointestinal (GI) endoscopy findings in patients with mesenteric panniculitis, compared to matched controls, to investigate their clinical outcomes including incidence of malignancy and mortality. Methods: Retrospective case–control study was conducted, and included patients who were diagnosed with mesenteric panniculitis according to Coulier radiologic criteria on abdominal computerized tomography between 1/2005 and 12/2019, and followed to 12/2021. The case group was compared to a matched control group without MP on abdominal CT. Clinical data and the upper and lower endoscopies’ reports were reviewed in both groups. We excluded patients who, beyond diagnosis of MP, were also diagnosed with current malignancy, significant intra-abdominal morbidity or inflammatory bowel disease. Results: The initial set of 376 patients with MP, after exclusion, included 187 patients. A total of 56.1% were male, with a mean age 60 ± 15 years. Of them, 74 (39%) patients underwent follow-up CT scans, which demonstrated, in 66 (89.2%) patients, a stable MP without any aggravation. Colonoscopy was performed in 89 MP patients, and 98/187 controls. No significant difference in the colonoscopies’ findings was found between the two groups. Gastroscopy was performed in 84 MP and 79 controls. No case of gastric cancer was found. No statistically significant difference was found in the rate of gastroscopy findings. By the end of the follow-up period, malignancy was diagnosed in four patients of the MP group. None were colon cancer. The mortality rate in the MP group was 3.2%, without a significant difference compared to the controls. None were MP related. Conclusions: MP identified on abdominal CT is not associated with pathologic endoscopy findings or future diagnosis of colon cancer, and also has no impact on mortality rate. Since repeating abdominal CT did not reveal any disease progression, the necessity of follow-up imaging for MP should be carefully reconsidered.
Comparison of Nitroglycerin-Induced Pressure Ratio Drop and Resting Full-Cycle Ratio in a Pressure Wire Study
Authors: Jong Chien-Boon , Lu Tsui-Shan , Liao Min-Tsun , Xu Jia-Lang , Chen Chun-Kai , Kuo Jui-Cheng , Wu Chih-Cheng
Published: November 2024

Background/Objectives: The acute drop in distal coronary pressure (Pd)-to-aortic pressure (Pa) ratio after intracoronary nitroglycerin (NTG-Pd/Pa) administration is an acceptable estimate of fractional flow reserve (FFR). We aimed to compare the diagnostic performance of NTG-Pd/Pa with that of the resting full-cycle ratio (RFR) in predicting the binary results of FFR. Methods: This study included two prospective studies registered under the numbers NCT04700397 and NCT03693157. Altogether, 202 vessels were included. The optimal cutoff of NTG-Pd/Pa for predicting FFR ≤ 0.8 was identified and validated in another prospective registry. We used the McNemar’s test and the DeLong method to compare the diagnostic efficiency of NTG-Pd/Pa vs. RFR in predicting FFR ≤ 0.8 in a pooled cohort. Results: NTG-Pd/Pa was strongly correlated with FFR (r = 0.945, p < 0.001). The NTG-Pd/Pa cutoff for predicting FFR ≤ 0.8 was 0.85 in both the derivation and validation cohorts. The area under the receiver-operating characteristic curve (AUC) and accuracy in predicting FFR ≤ 0.8 were higher for NTG-Pd/Pa than for RFR in the pooled cohort (AUC 0.97 vs. 0.91, p < 0.001; accuracy 91% vs. 84%, p < 0.001). The sensitivity and negative predictive values were also higher for NTG-Pd/Pa than for RFR (all p < 0.05). The specificity and positive predictive value were numerically higher for NTG-Pd/Pa than for RFR (all p > 0.05). Conclusions: The diagnostic performance of NTG-Pd/Pa may surpass that of the RFR in predicting the binary results of the FFR.
Arnold–Chiari Malformations in Pediatric Patients After Fetal Surgery for Meningomyelocele
Authors: Kohútková Miroslava , Horn František
Published: November 2024

(1) Background: Fetal surgery for meningomyelocele (MMC) should reduce the occurrence of Arnold–Chiari malformations, hydrocephalus, the associated need for craniocervical decompression, and the need for cerebrospinal fluid shunt insertion. Fetal surgery should improve ambulatory status. (2) Methods: We used retrospective analysis of the documentation and descriptive statistics to summarize the clinical data and measured MRI parameters. The neurosurgical results are presented as the frequency of findings in percentages and compared with the results of the Management of Myelomeningocele Study (MOMS). (3) Results: A total of eight patients who underwent prenatal correction of MMC between 2016 and 2020 participated. MRI detected Chiari II malformation in all patients during prenatal imaging and in 87.5% of the patients at the age of 12 months. Craniocervical decompression was used in 25% of the patients. Shunt-dependent hydrocephalus occurred in 50% of the cases. In 87.5% of the cases, the functional level exceeded the expected ambulatory status. (4) Conclusions: We present the clinical status of our patients. Analysis of the complete cohort confirmed that prenatal surgery is associated with a reduced occurrence of Chiari malformations and reduced associated occurrence of hydrocephalus. Specific lesion levels are not associated with the need for craniocervical decompression. The results of our study are valuable in prenatal counseling and important for treatment planning.
The Relationship Between Hand Abnormalities and Diabetic Retinopathy in Patients with Type 2 Diabetes Mellitus
Authors: Uzun Feyzahan , Fındık Hüseyin , Kaim Muhammet , Yıldırım Murat
Published: November 2024

Background: The link between diabetes mellitus (DM) and pathological conditions of the hand has been previously investigated. Retinopathy is one of the most common microvascular complications of DM. In this study, we aimed to evaluate the relationship between hand abnormalities and retinopathy in patients with type 2 DM. Methods: Patients with type 2 DM were assessed for hand abnormalities using tactile, functional, and sensory tests. The patients were evaluated electrodiagnostically for carpal tunnel syndrome and polyneuropathy (PNP). A comprehensive ophthalmologic examination was performed to diagnose diabetic retinopathy (DR). Subjective disability and quality of life were evaluated using the Quick DASH questionnaire. The duration of the disease and HbA1c levels were noted. Results: A total of 60 patients with type 2 DM (32 female, 28 male) were recruited for this study. The mean age was 55.1 ± 5.6 years. Among the 60 diabetic patients examined, 15 (25%) were diagnosed with DR. The mean duration of DM was 86.86 ± 51.69 months in patients without DR and 144.75 ± 82.96 months in patients with DR. The mean HbA1c level was 8.86% in the DR group and 8.64% in the non-DR group. PNP was the only hand abnormality that showed a significant association with retinopathy (p = 0.011). Conclusions: PNP might be used as a clue to the presence of retinopathy, especially in long-lasting diabetes. Particular attention should be given to hand abnormalities, especially in patients with PNP, due to their association with DR.
Clinical and Microbiological Periodontal Biofilm Evaluation of Patients with Type I Diabetes
Authors: Maris Mihaela , Martu Maria-Alexandra , Maris Marius , Martu Cristian , Anton Diana Maria , Pacurar Mariana , Earar Kamel
Published: November 2024

Background/Objectives: The purpose of this study was to assess the microbial composition and density of subgingival plaque samples for periodontal pathogens while correlating the values with glycemic control levels via glycated hemoglobin (HbA1c), a type of hemoglobin that has chemically linked glucose, in type I diabetes individuals who will undergo complex oral rehabilitation through orthodontic treatment and implant surgery. Methods: A cohort of 42 adults with type I diabetes were included in this study. The subjects sustained a comprehensive periodontal clinical examination as well as microbiological assessments of their subgingival plaque samples through quantitative real-time PCR. The samples were collected from the two deepest pockets of each subject. Results: The highest number of periodontopathogenic bacteria was observed in the pockets of 5–7 mm. T. forsythia showed the highest prevalence (20.48%), with decreasing numbers as follows: T. denticola (13.31%), P. gingivalis (11.26%), A. actinomycetemcomitans (7%), and P. intermedia (4.9%). T. denticola and T. forsythia were significantly more commonly observed in individuals with elevated HbA1c serum levels. No correlation was observed between P. gingivalis, A. actinomycetemcomitans, P. intermedia presence, and the HbA1c value. Conclusions: Periodontopathogenic agents’ presence in subgingival biofilm samples varied in accordance with the pocket probing depth and metabolic control of the diabetic individuals. In our study, the appearance of these periodontopathogenic agents was linked to lowered metabolic control in patients with type I diabetes mellitus.
Early Acute Kidney Injury in Stroke Patients Submitted to Endovascular Treatment: A Cohort Study
Authors: Oliveira Marta , Sousa Miguel , Antunes Rita , Macedo Diogo , Belchior Sabina , Soares Daniela , de Oliveira Simões Francisco , Rocha Mariana , Costa Henrique , Novo Joana , Paredes Ludovina , Barros Pedro , Pires Pedro , Castro Sérgio , Ribeiro Manuel , Araújo André , Afreixo Vera , Gregorio Tiago
Published: November 2024

Background/Objectives: Acute kidney injury (AKI) is a potential complication of cardiovascular disorders and is associated with worse outcome. The aim of this study was to assess the incidence of early AKI after endovascular therapy for acute ischemic stroke, identify predictors for this complication, and test the association between AKI and mortality or death or dependency. Methods: This was a single-center cohort study involving consecutive patients with acute ischemic stroke submitted to endovascular therapy between 2015 and 2022. AKI was defined according to the KDIGO criteria and evaluated at 48 h. Other outcomes of interest were vital status and functional dependency at 3 months using the modified Rankin Scale, with death or dependency being defined as a score > 2. An adjustment for potential confounders was performed using logistic regression. Results: Overall, 1150 patients were included in the analysis, with a mean age of 74 years and a slight female preponderance (56%). The median NIHSS was 15, the mean onset-to-groin time was 392 min, and 92% of patients were successfully recanalized. The overall incidence rate of AKI was 6%. On univariate analysis, patients with AKI were older (p = 0.002), had a longer time to EVT (p = 0.042), higher NIHSS (p = 0.006), higher blood glucose (p = 0.033), and lower baseline glomerular filtration rate (GFR) (p < 0.001). After adjustment for confounders, AKI was independently associated with NIHSS (p = 0.012), time to treatment (p = 0.004), and lower baseline GFR (p < 0.001). AKI was also independently associated with higher mortality (OR = 2.302, p = 0.003). Conclusions: Patients with impaired baseline renal function and more severe stroke are at higher risk of AKI, and AKI begets worse stroke outcome. Better strategies are required to optimize treatment outcome in these patients and avert this vicious cycle.
Trade-Off Between Wear/Corrosion Performance and Mechanical Properties in D-AlNiCo Poly-Quasicrystals Through CNT Addition to the Microstructure
Authors: Hosseini Seyedmehdi , Novák Pavel , Alishahi Mostafa , Kačenka Zdeněk , Šittner Petr
Published: November 2024

An ultrafine-grained Al71Ni14.5Co14.5/CNT poly-quasicrystal (QC/CNT) composite was synthesized using spark plasma sintering of powder components developed through electroless Ni-P/CNT plating of Co particles and mechanical alloying. The performance of the synthesized samples was studied using various testing methods, such as room temperature/hot compression, wear, and corrosion tests. The results were compared to the properties of alloy samples fabricated from raw and coated powders (without CNTs). The wear rate and friction coefficient of the quasicrystalline samples improved significantly due to the contribution of the CNTs. The wear rate of the CNT-containing specimens was 0.992 × 10−4 mm3/N/m, which is 47.1% lower than that of the QC sample. The positive impact of the CNTs on the corrosion potential and current density was further validated by the potentiodynamic polarization tests in a saline solution. However, these improvements in surface properties came at the cost of a 21.5% reduction in compressive strength, although the compressive strength still remained above 1.1 GPa at 600 °C. The results highlight an interesting trade-off between surface properties and mechanical strength, pointing toward the development of materials suitable for extreme conditions.
A Comparative Study on Laser Cutting Performance with Varying Speeds at 10 M Underwater
Authors: Song Danbi , Choi Jungsoo , Lee Sujin , Kim Ryoonhan , Park Induck
Published: November 2024

Despite the dismantling structures that are submerged to significant depths of water during the decommissioning of nuclear power plants, there is limited research on deep-water laser cutting processes. A self-designed pressurized chamber was used in this study and successfully conducted the world’s first laser cutting experiment in a simulated 10 m water depth environment. laser cutting was performed in a 10 m underwater environment, and the cutting efficiency was compared to that observed in a 1 m underwater environment. Therefore, A 100 mm thickness of 304 stainless steel was successfully cut underwater, and the highest cutting speed of 100 mm/min was achieved. The result indicates that, as the cutting speed increased during underwater laser cutting, both the heat input and the mass flow rate of the assist gas decreased, resulting in a narrower rear kerf width and an ineffective evacuation of the molten metal.
Targeting Disulfidptosis with Potentially Bioactive Natural Products in Metabolic Cancer Therapy
Authors: Li Xinyan , Xu Jiayi , Yan Liangwen , Tang Shenkang , Zhang Yinggang , Shi Mengjiao , Liu Pengfei
Published: November 2024

Background: Metabolic cancers are defined by metabolic reprogramming. Although this reprograming drives rapid tumour growth and invasion, it also reveals specific metabolic vulnerabilities that can be therapeutically exploited in cancer therapy. A novel form of programmed cell death, known as disulfidptosis, was identified last year; tumour cells with high SLC7A11 expression undergo disulfidptosis when deprived of glucose. Natural products have attracted increasing attention and have shown potential to treat metabolic cancers through diverse mechanisms. Methods: We systematically searched electronic databases involving PubMed, Web of Science, Gooale Scholar. To ensue comprehensive exploration, keywords including metabolic reprogramming, metabolic cancer, disulfidptosis, natural products and some other words were employed. Results: In this review, we focus on the shared characteristics and metabolic vulnerabilities of metabolic cancers. Additionally, we discuss the molecular mechanisms underlying disulfidptosis and highlight key regulatory genes. Furthermore, we predict bioactive natural products that target disulfidptosis-related genes, offering new perspectives for anticancer strategies through the modulation of disulfidptosis. Conclusions: By summarizing current research progress, this review mainly analyzed the potential mechanisms of natural products in the treatment of metabolic cancer.
The CH3D Absorption Spectrum Near 1.58 μm: Extended Line Lists and Rovibrational Assignments
Authors: Ben Fathallah Ons , Lembei Anastasiya , Rey Michael , Mondelain Didier , Campargue Alain
Published: November 2024

Monodeuterated methane (CH3D) contributes greatly to absorption in the 1.58 μm methane transparency window. The spectrum is dominated by the 3ν2 band near 6430 cm−1, which is observed in natural methane and used for a number of planetary applications, such as the determination of the D/H ratio. In this work, we analyze the CH3D spectrum recorded by high-sensitivity differential absorption spectroscopy in the 6099–6530 cm−1 region, both at room temperature and at 81 K. Following a first contribution to this topic by Lu et al., the room-temperature line list is elaborated (11,189 lines) and combined with the previous 81 K list (8962 lines) in order to derive about 4800 empirical lower-state energy values from the ratio of the line intensities measured at 81 K and 294 K (2T-method). Relying on the position and intensity agreements with the TheoReTS variational line list, about 2890 transitions are rovibrationally assigned to twenty bands, with fifteen of them being newly reported. Variational positions deviate from measurements by up to 2 cm−1, and the band intensities are found to be in good agreement with measurements. All the reported assignments are confirmed by Ground-State Combination Difference (GSCD) relations; i.e., all the upper-state energies (about 1370 in total) have coinciding determinations through several transitions (up to 8). The energy values, determined with a typical uncertainty of 10−3 cm−1, are compared to their empirical and variational counterparts. The intensity sum of the transitions assigned between 6190 and 6530 cm−1 represents 76.9 and 90.0% of the total experimental intensities at 294 K and 81 K, respectively.
Simulation Study of High-Precision Characterization of MeV Electron Interactions for Advanced Nano-Imaging of Thick Biological Samples and Microchips
Authors: Yang Xi , Wang Liguo , Smaluk Victor , Shaftan Timur , Wang Tianyi , Bouet Nathalie , D’Amen Gabriele , Wan Weishi , Musumeci Pietro
Published: November 2024

The resolution of a mega-electron-volt scanning transmission electron microscope (MeV-STEM) is primarily governed by the properties of the incident electron beam and angular broadening effects that occur within thick biological samples and microchips. A precise understanding and mitigation of these constraints require detailed knowledge of beam emittance, aberrations in the STEM column optics, and energy-dependent elastic and inelastic critical angles of the materials being examined. This simulation study proposes a standardized experimental framework for comprehensively assessing beam intensity, divergence, and size at the sample exit. This framework aims to characterize electron-sample interactions, reconcile discrepancies among analytical models, and validate Monte Carlo (MC) simulations for enhanced predictive accuracy. Our numerical findings demonstrate that precise measurements of these parameters, especially angular broadening, are not only feasible but also essential for optimizing imaging resolution in thick biological samples and microchips. By utilizing an electron source with minimal emittance and tailored beam characteristics, along with amorphous ice and silicon samples as biological proxies and microchip materials, this research seeks to optimize electron beam energy by focusing on parameters to improve the resolution in MeV-STEM/TEM. This optimization is particularly crucial for in situ imaging of thick biological samples and for examining microchip defects with nanometer resolutions. Our ultimate goal is to develop a comprehensive mapping of the minimum electron energy required to achieve a nanoscale resolution, taking into account variations in sample thickness, composition, and imaging mode.
Characterization and Differentiation of Candida auris on Dixon’s Agar Using Raman Spectroscopy
Authors: Petrokilidou Chrysoula , Pavlou Eleftherios , Velegraki Aristea , Simou Anna , Marsellou Ioanna , Filis Grigorios , Bassukas Ioannis D. , Gaitanis Georgios , Kourkoumelis Nikolaos
Published: November 2024

Candida auris, an emerging multidrug-resistant fungal pathogen, poses significant challenges in healthcare settings due to its high misidentification rate and resilience to treatments. Despite advancements in diagnostic tools, a gap remains in rapid, cost-effective identification methods that can differentiate C. auris from other Candida species, particularly on non-standard culture media. We used Raman spectroscopy to characterize C. auris grown on modified Dixon’s agar (mDixon) and differentiated it from Candida albicans and Candida parapsilosis. Key Raman spectral markers at 1171 cm−1 and 1452 cm−1, linked to mannan and β-glucan composition, differentiated C. auris into two subgroups, A and B. Despite the spectral similarities of groups A and B with C. albicans and C. parapsilosis, respectively, all Candida species were distinguishable through principal component analysis (PCA). Additionally, this study is the first to demonstrate the distinct spectral signature of mDixon agar, achieved through spatially offset Raman spectroscopy (SORS), which enables accurate discrimination between the culture medium and fungal samples. The observed inter-individual variability within C. auris, coupled with the spectral overlap between C. auris subgroups and other Candida species, highlights a major challenge in differentiating closely related fungi due to their similar molecular composition. Enhancements in spectral resolution and further fluorescence minimization from the culture medium are needed to reliably detect the subtle biochemical differences within these species. Despite these challenges, the results underscore the potential of Raman spectroscopy as a real-time, non-destructive, and complementary tool for fungal pathogen identification.