bluuebunny/crossref_metadata_2025 huggingface dataset:
Polysomal mRNA Association and Gene Expression in Trypanosoma brucei
Authors: Tinti Michele , Kelner-Mirôn Anna , Marriott Lizzie J. , Ferguson Michael A.J.
Published: August 2021

Background: The contrasting physiological environments of Trypanosoma brucei procyclic (insect vector) and bloodstream (mammalian host) forms necessitates deployment of different molecular processes and, therefore, changes in protein expression. Transcriptional regulation is unusual in T. brucei because the arrangement of genes is polycistronic; however, genes which are transcribed together are subsequently cleaved into separate mRNAs by trans-splicing. Following pre-mRNA processing, the regulation of mature mRNA stability is a tightly controlled cellular process. While many stage-specific transcripts have been identified, previous studies using RNA-seq suggest that changes in overall transcript level do not necessarily reflect the abundance of the corresponding protein.

Methods: To better understand the regulation of gene expression in T. brucei, we performed a bioinformatic analysis of RNA-seq on total, sub-polysomal, and polysomal mRNA samples. We further cross-referenced our dataset with a previously published proteomics dataset to identify new protein coding sequences.

Results: Our analyses showed that several long non-coding RNAs are more abundant in the sub-polysome samples, which possibly implicates them in regulating cellular differentiation in T. brucei. We also improved the annotation of the T.brucei genome by identifying new putative protein coding transcripts that were confirmed by mass spectrometry data.

Conclusions: Several long non-coding RNAs are more abundant in the sub-polysome cellular fractions and might pay a role in the regulation of gene expression. We hope that these data will be of wide general interest, as well as being of specific value to researchers studying gene regulation expression and life stage transitions in T. brucei.
Increase in blood glucose level and incidence of diabetic ketoacidosis in children with type 1 diabetes mellitus in the Daegu-Gyeongbuk area during the coronavirus disease 2019 (COVID-19) pandemic: a retrospective cross-sectional study
Authors: Lee Mi Seon , Lee Rosie , Ko Cheol Woo , Moon Jung Eun
Published: August 2021

Background: The coronavirus disease 2019 (COVID-19) outbreak in the Daegu-Gyeongbuk area in 2020 has caused difficulties in the daily life and hospital care of children with type 1 diabetes mellitus (T1DM). We detected an increase in blood sugar levels in these children and the number of patients hospitalized with more severe diabetic ketoacidosis (DKA) compared to those before COVID-19. Methods: This single-center study was conducted at Kyungpook National University Children’s Hospital. The following patient groups were included; 45 returning patients diagnosed with T1DM and undergoing insulin treatment for more than 2 years and 20 patients newly diagnosed with T1DM before and after COVID-19 were selected by age matching. Returning patients before and after the outbreak were selected, and changes in hemoglobin A1c (HbA1c) levels were retrospectively reviewed. The HbA1c levels and severity of symptoms in newly diagnosed patients during hospitalization were examined. Results: HbA1c levels in returning patients with T1DM were significantly increased after COVID-19 (before, 7.70%±1.38% vs. after, 8.30%±2.05%; p=0.012). There were 10 and 10 newly diagnosed patients before and after COVID-19, respectively. The proportion of patients with drowsiness and dyspnea at the time of admission was higher after COVID-19 than before (before, 2 of 10 vs. after, 4 of 10). The HbA1c levels were higher in newly diagnosed patients hospitalized after COVID-19 than before (before, 11.15% vs. after, 13.60%; p=0.036). Conclusion: Due to COVID-19 in the Daegu-Gyeongbuk area, there was an increase in blood glucose levels in children with T1DM and in the incidence of severe DKA in newly diagnosed diabetes mellitus patients.
Evaluation of Physical and Psychosocial Well-Being of Personnel: Employees’ Self-Feeling and Presenteeism at Work
Authors: Frelih M. , Fedorova A.
Published: August 2021

The article is devoted to the study of factors that have a negative impact on the well-being of employees in the workplace. Special attention is paid to the problem of presenteeism on the example of a large metallurgical enterprise. A review of foreign and domestic publications allows concluding that until now specialists do not have reliable and valid tools for studying the presenteeism phenomenon in organizations. The purpose of the research presented in the paper is to examine influence of the factors of the intra-organizational environment on the personnel well-being and assess the level of presenteeism at the enterprise. Empirical data were obtained by conducting a sociological survey of various categories of workers, as well as assessing the impact of presenteeism on the economic indicators of the studied enterprise. For the subsequent in-depth study of health problems in the workplace, the authors have developed a research tool based on the use of a digital service, which allows monitoring the self-feeling of employees by the self-assessment method, which determines the level of physical and psychosocial well-being of staff.
Topographic and Histologic Analysis of the Collateral Ligament Complex around the Elbow
Authors: Kim Jong-Pil , Park Ji-Kang , Yoo Joon-Young , Shin Won-Jeong , Kim Jeong-Sang , Kim Young-IL
Published: August 2021

Purpose: The purpose of this study was to evaluate topographic anatomy of the footprints of key ligaments of the elbow and assess their relationships with bony parameters using micro-computed tomography (micro-CT). Additionally, the ratios of type I/III collagen at the medial collateral ligament (MCL) and lateral collateral ligament (LCL) of elbow were investigated.Methods: Eleven cadaveric elbows attached by both the MCL and LCL were scanned using micro-CT and reconstructed three-dimensionally. Additionally, the ligaments were examined under polarized light microscopy to determine the histological characteristics of collagen patterns. Results: Areas of footprints of the MCL and LCL attaching onto the humerus were 133.2±25.8 mm² and 128.3±23.2 mm², respectively. Footprint sizes of anterior and posterior bundles of the MCL in the proximal ulna and lateral ulnar collateral ligament (LUCL) attaching to the proximal ulna averaged to 109.9 mm², 89.2 mm², and 89.7 mm², respectively. There were a positive correlation between footprint size of the MCL and LUCL at the humeral side and a negative correlation between the footprint size of the MCL at humeral side and maximal diameter of the radial head. The collagen I/III ratio of the humeral attachment of the MCL was higher than distal attachment of the MCL. Conclusion: This study provides a better understanding of the pathologies of the MCL and LCL complex of the elbow and their relationships with osseous anatomy and may assist the clinician with an anatomic reconstruction of the ligaments.
An Efficient Label-Correcting Algorithm for the Multiobjective Shortest Path Problem
Authors: Kergosien Yannick , Giret Antoine , Néron Emmanuel , Sauvanet Gaël
Published: August 2021

This paper proposes an exact algorithm to solve the one-to-one multiobjective shortest path problem. The solution involves determining a set of nondominated paths between two given nodes in a graph that minimizes several objective functions. This study is motivated by the application of this solution method to determine cycling itineraries. The proposed algorithm improves upon a label-correcting algorithm to rapidly solve the problem on large graphs (i.e., up to millions of nodes and edges). To verify the performance of the proposed algorithm, we use computational experiments to compare it with the best-known methods in the literature. The numerical results confirm the efficiency of the proposed algorithm.

Summary of Contribution: The paper deals with a classic operations research problem (the one-to-one multiobjective shortest path problem) and is motivated by a real application for cycling itineraries. An efficient method is proposed and is based on a label-correcting algorithm into which several additional improvement techniques are integrated. Computational experiments compare this algorithm with the best-known methods in the literature to validate the performance on large-size graphs (Center for Discrete Mathematics and Theoretical Computer Science (DIMACS) instances from the ninth DIMACS challenge). New instances from the context of cycling itineraries are also proposed.
Severe Haemolytic Anaemia Resulting from a Malpositioned Haemodialysis Catheter
Authors: Ammad Ud Din Mohammad , Liaqat Hania , Gill Prabhsimrat , Low Soon Khai
Published: August 2021

Severe haemolytic anaemia is a rare complication of haemodialysis that is often difficult to recognize, especially when there are other potential differential diagnoses. Here, we present the case of 19-year-old man on haemodialysis who developed severe haemolytic anaemia while recovering from acute renal failure secondary to rhabdomyolysis. Other causes of haemolytic anaemia such as thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome were ruled out. As his blood counts were dropping on days following haemodialysis, haemolysis secondary to the mechanical sheering effect of the catheter was considered and his haemodialysis catheter was exchanged, which led to the resolution of anaemia.
Kajian Literatur: Hubungan Personal Higiene Penjamah Makanan dengan Kontaminasi Escherichia Coli pada Makanan
Authors: Utami Anggun Putri
Published: August 2021

Kontaminasi Escherichia Coli pada makanan dapat menimbulkan adanya KLB keracunanan makanan atau KLB diare di Indonesia. Personal higiene penjamah makanan diketahui berperan penting dalam mencegah adanya kontaminasi tersebut. Studi literatur ini digunakan untuk mengetahui hubungan personal higiene penjamah makanan dengan kontaminasi E. coli pada makanan. Jurnal terkait hubungan personal higiene penjamah makanan dengan kontaminasi E. coli pada makanan dicari menggunakan situs ScienceDirect, Google Scholar, Pubmed, dan Portal garuda. Kriteria inklusi adalah jurnal yang berkaitan dengan personal higiene penjamah makanan dan kontaminasi E. coli, menggunakan sampel makanan dan penjamah makanan di tempat pengelolaan makanan (TPM), jurnal terindeks, jurnal yang dapat diakses secara penuh dan merupakan jurnal publikasi sepuluh tahun terakhir. Didapatkan lima dari 1.320 jurnal yang dapat dianalisis lebih lanjut. Berdasarkan lima jurnal yang dianalisis, dua jurnal menyebutkan bahwa personal higiene memiliki hubungan dengan kontaminasi E. coli pada makanan. Tiga jurnal lain memiliki variabel berbeda, yaitu praktik cuci tangan dan atau kebesihan diri penjamah makanan yang mana kedua variabel tersebut masuk ke dalam parameter personal higiene penjamah makanan. Ketiga variabel tersebut memiliki hubungan dengan kontaminasi E. coli pada makanan. Personal higiene penjamah makanan memiliki hubungan dengan kontaminasi E. coli pada makanan.
Assessing the utilization of naturalized alien plant species by community to inform its management strategy: A case study in Cibodas Biosphere Reserve, West Java, Indonesia
Authors: Handayani Aisyah , Zuhud Ervizal A. M. , Junaedi Decky Indrawan
Published: August 2021

Abstract. Handayani A, Zuhud EAM, Junaedi DI. 2021. Assessing the utilization of naturalized alien plant species by community to inform its management strategy: A case study in Cibodas Biosphere Reserve, West Java, Indonesia. Biodiversitas 22: 2579-2588. Alien plant species can have two side effects, either positively in terms of the utilization of these species to fulfill human needs, or negatively in terms of their invasion which might threaten biodiversity and reduce land productivity. Yet, it is not clear how an introduced plant species can be carefully managed and utilized (so-called Naturalized Alien Plant Species/NAPS) so that the NAPS does not become Invasive Alien Plant Species (IAPS). Using Cibodas Biosphere Reserves (CBR), West Java, Indonesia as a case study, this study aims to investigate the utilization of Naturalized Alien Plant Species (NAPS) by local communities in the buffer zone of CBR and quantify the utilization of NAPS based on cultural perspective using Index Cultural Significance (ICS) framework. Data were collected by distributing questionnaires and interviewing 90 respondents in three locations around CBR: Bodogol, Cibodas, and Gekbrong. The results showed that the community used 72 Naturalized Alien Plant Species, with 30% of the utilized plants were from Asteraceae family. Centella asiatica was the most widely used species, but the species with the highest Index Cultural Significance value was Artemisia vulgaris. Based on the ICS classification, there were ten species (13.89%) with high significance values, 17 species (23.61%) with moderate significance, 30 species (41.67%) with low significance, and 15 species (20.83%) with very low significance. Species with high significance values were Artemisia vulgaris, Chimonobambusa quadrangularis, Bidens pilosa, Mentha arvensis, Amaranthus spinosus, Centella asiatica, Cyphomandra betacea, Calliandra calothyrsus, Fragaria vesca, and Solanum torvum. Based on these results, the recommended strategy is to regularly harvest the Naturalized Alien Plant Species with high ICS value to control their population and fulfill community needs.
Short Communication: Analysis of the chromosome numbers of Zinnia elegans Jacq. in single, double, and pom-pom flowers
Authors: SAIFUDIN Saifudin , Shafira Syalwa , Dwiranti Astari , Salamah Andi
Published: August 2021

Abstract. Saifudin, Shafira S, Dwiranti A, Salamah A. 2021. Short Communication: Analysis of the chromosome numbers of Zinnia elegans Jacq. in single, double, and pom-pom flowers. Biodiversitas 22: 2771-2777. Zinnia elegans Jacq. is highly valued as an ornamental plant with a variety of flower colors, sizes, and shapes. Polyploidization has been reported in Z. elegans with pom-pom flowers, nevertheless, the variation in chromosome numbers of various flower shapes has yet to be investigated. This study aimed to analyze the chromosome numbers of Z. elegans Jacq. cultivar “California Giant,” “Lilliput,” and “Cactus Flowered Mix” with single, double, and pom-pom flowers to determine their variations and identify the morphology of the flowers. Chromosomes were prepared using the squashing method, and images were analyzed using the Chromosome Image Analyzing System (CHIAS) IV. The minimum of 5 slides was prepared for each flower type from each cultivar. The results show that the three cultivars are diploid plants (2n = 24) with varying flower morphology. The single and double flowers of Z. elegans “Lilliput” and the single flower of “Cactus Flowered Mix” showed no variation in chromosome numbers. In contrast, chromosome number variation was found in the pom-pom flower of Z. elegans “California Giant” (2n = 22, 24, 48) and the double flower of “Cactus Flowered Mix” (2n = 9, 13, 15, 24). Two cultivars, Z. elegans “California Giant” and Z. elegans “Cactus Flowered Mix,” were successfully analyzed using CHIAS IV. Statistical analysis using a t-test (? = 0.05) showed that the total chromosome length of Z. elegans “California Giant” (2n = 24) was significantly greater than that of Z. elegans “Cactus Flowered Mix” (2n = 24). Chromosome satellites were found in both cultivars.
Local knowledge and practices towards the ecological restoration of selected landscape in Atok, Benguet, Philippines
Authors: LANDICHO LEILA , Ocampo Maria Theresa Nemesis , Cabahug Rowena Esperanza , Abadillos Maryanne , Cosico Russel Son , Castillo Arnold Karl , Ramirez Ma. Armie Janica , Laruan Kenneth
Published: August 2021

Abstract. Landicho LD, Ocampo MTNP, Cabahug RED, Abadillos MG, Cosico RSA, Castillo AKA, Ramirez MAJP, Laruan KA. 2021. Local knowledge and practices towards the ecological restoration of selected landscape in Atok, Benguet, Philippines. Biodiversitas 22: 2785-2794. This study argues that local ecological knowledge and practices contribute to forest conservation and management efforts. This argument is based on the research conducted in upland communities in Atok, Benguet, Philippines. Interviews, farm visits, and focus group discussions revealed that the local communities have been dependent on the forest resources within the watershed in their agricultural production activities. These local communities employed their knowledge and practices towards restoring the ecological state of the watershed. These include the integration of trees on the farm, planting of Alnus japonica for soil and water conservation, the establishment of structural soil and water conservation, maintenance of communal forests, and awareness building among the youth on forest conservation and management. A moderate level of biodiversity and evenness index were in the conservation of giant tree ferns. A very low (0.9718) diversity index and a very high (0.825) evenness index were recorded in the farm lots, while a very low (0.437) diversity index and moderate (0.421) evenness index were measured in the communal forests. On the other hand, high soil organic matter contents of 6.49% and 5.86% were recorded both at the community and farm lots, respectively. Results imply the need to sustain the use of local knowledge and practices in combination with the technological interventions from academia and research institutions to enhance the ecological restoration of forest landscapes.
Short Communication: In vitro antimicrobial and antimalarial screening of a crude extract of Streptomyces sp. AB8 isolated from Lapindo Mud Volcano Area, Sidoarjo, Indonesia
Authors: Arifiyanto Achmad , Setyaningrum Endah , Nukmal Nismah , Aeny Titik Nur
Published: August 2021

Abstract. Arifiyanto A, Setyaningrum E, Nukmal N, Aeny TN. 2021. Short Communication: In vitro antimicrobial and antimalarial screening of a crude extract of Streptomyces sp. AB8 isolated from Lapindo Mud Volcano Area, Sidoarjo, Indonesia. Biodiversitas 22: 2817-2823. Streptomyces is a potential bacterial genus that has been investigated extensively as a source of natural microbial compounds. Its potential metabolites have been widely developed for pharmaceutical, pathogen control, and other applications in agriculture. This study aimed to determine the ability of the Streptomyces sp AB8 crude extract in inhibiting Plasmodium and pathogenic microbes.  Streptomyces was cultured on Gause synthetic media for 10 days. The fermented broth culture media has dissolved in a 1:1 mixture of ethyl acetate and methanol. Biochemical characterization of this isolate has carried out using the standard methods. In-vitro antimalarial activity assay was performed using a chloroquine-sensitive Plasmodium falciparum strain 3D7. Fresh type O-positive human erythrocytes were suspended at 4 percent hematocrit in a complete medium to maintain culture. The inhibitory concentration (IC50) was determined using probit analysis. The results showed the extract of Streptomyces sp. AB8 contains phenolic and alkaloids. Streptomyces sp. AB8 extract can inhibit Dickeya zeae N-Unila 5, Dickeya zeae N-Unila 10, Aspergillus sp IK3, and Escherichia coli growth. The results also showed that the ICs0 value of extract against P. falciparum 3D7 was 17.56 ug/mL. Further research was needed to determine the types of purified bioactive compounds and their bioactivity.
Monthly Chaetocerotales diversity and abundance, and its relationship with water physicochemical parameters and phytoplankton diversity in Carey Island mangrove ecosystem, Malaysia
Authors: REDZUAN NURUL SHAHIDA , Milow Pozi
Published: August 2021

Abstract. Redzuan NS, Milow P. 2021. Monthly Chaetocerotales diversity and abundance, and its relationship with water physicochemical parameters and phytoplankton diversity in Carey Island mangrove ecosystem, Malaysia. Biodiversitas 22: 2919-2927. Globally, phytoplankton of order Chaetocerotales has been reported to cause monospecific bloom events. The blooms that normally lead to ecosystem instability, also physically responsible for histological and physical damages to fishes. Regarding that order Chaetocerotales often display significant temporal variability, this study aimed to investigate the monthly variability of species composition and cell density of phytoplankton of order Chaetocerotales during high tide period immersion were investigated over a year period. In addition, this present study also aimed to analyze the relationship between Chaetocerotales diversity and abundance with physical and chemical parameters that potentially controlling their temporal variability. Samplings were carried out from April 2009 until March 2010 in the mangrove ecosystem of Carey Island, Selangor, Malaysia. Of 84 phytoplankton taxa recorded, 18 species belonged to order Chaetocerotales, i.e. the genera Bacteriastrum and Chaetoceros. Although initially the sampling was designed to study the overall phytoplankton, the study also aimed at high temporal variability in Chaetocerotaceae cells density has called for this paper to be written. Interestingly, order Chaetocerotales showed to have negative correlation with the total phytoplankton species. Chaetocerotales blooms at the study site were represented by multispecies blooms, with Chaetoceros curvisetus as the highest species in the events. Dissolved oxygen, on the other hand, showed to have negative correlation Chaetocerotales' cells density. All of the species, except the Chaetoceros subtilis and Chaetoceros neglectus displayed significant temporal monthly variability across twelve months of sampling. Nitrate, phosphate and temperature are potentially the parameters that stimulate the growth of the order, consequently causing bloom events.
Epiphytic microalgae community as aquatic bioindicator in Brantas River, East Java, Indonesia
Authors: Arsad Sulastri , Putra Khabib Taufiq , Latifah Nurul , Kadim Miftahul Khair , Musa Muhammad
Published: August 2021

Abstract. Arsad S, Putra KT, Latifah N, Kadim MK, Musa M. 2021. Epiphytic microalgae community as aquatic bioindicator in Brantas River, East Java, Indonesia. Biodiversitas 22: 2961-2971. One way to monitor water quality is by using biological indicators, namely epiphytic microalgae (periphyton). This study aims to analyze the epiphytic periphyton community structure and analyze the river health status using a saprobic index. The research location was in the Brantas River, Blitar District, East Java, Indonesia. The method used was a survey with sampling at three sites based on the purposive sampling technique. Periphyton samples were taken using the quadrant transect (5x5 cm²) method and then identified using the Lackey Drop Micro transect Counting Method. The results show that the periphyton community structure is in balance, and there are six divisions of 59 different genera. The six divisions found are Bacillariophyta, Cyanophyta, Chlorophyta, Charophyta, Ochrophyta, and Rhodophyta. The most identified genus is from the Bacillariophyta Division with 28 genera, while the least identified genus is the Rhodophyta division with 1 genus. The abundance ??ranges from 242,800-1,229,174 cells. cm-2, the relative abundance index ??of periphyton ranges from 3-60% (site 1), 9-57% (site 2), 1-62% (site 3), the diversity index ranges from 1.212-2.617 (moderate), the uniformity index ranges from 0.307-2.151 (high), the dominance index ranges from 0.105-0.549 (moderate), and the saprobic index ranges from-0.18 to 0.55. Moreover, supporting water quality parameters are still optimal, except for ammonia and total organic matter parameters that exceed the quality standard. Based on the saprobic value obtained, the water quality of the Brantas River in Tawangrejo Village can be classified into the category of ?/?-mesosaprobic to ?-mesosaprobic saprobic levels with mild to moderate levels of pollution.
Gender perspectives on agroforestry practices in Mt. Merapi hazards and risks prone area of Indonesia
Authors: Rozaki Zuhud , Rahmawati Nur , Wijaya Oki , Safitri Fadhilah , Senge Masateru , Kamarudin Mohd Fauzi
Published: August 2021

Abstract. Rozaki Z, Rahmawati N, Wijaya O, Safitri F, Senge M, Kamarudin MF. 2021. Gender perspectives on agroforestry practices in Mt. Merapi hazards and risks-prone area of Indonesia. Biodiversitas 22: 2980-2987. Despite the risk and hazards of the Mt. Merapi prone area, people continue to stay in the area as Agroforestry Adopters. This study aims to analyze the gender perspectives on agroforestry (AF) practices in the Mt. Merapi prone area. 128 men and 86 women were randomly selected from four different areas. Descriptive analysis, independent t-test, and logistic multinomial regression were used to analyze the data. Gender issue in AF practices is found in some parts of the practices. For example, women are more predominant in marketing the products rather than men, whilst the men are predominant in activities that need more strength such as planting. An external factor that is significant is the different impacts of farmers support groups on men and women. Meanwhile, factors such as capital access and AF easiness do not show significant differences between men and women. In other words, these two variables are felt the same among men and women when practicing AF. As for the contribution of AF, social aspect is found to show significant differences, whilst economic and ecology do not show significant differences to both the men and women. Additionally, factors that affect the perspectives on AF contribution vary among the respondents across both groups of men and women. This study suggests that the different perspectives and factors that are affecting AF farmers can be used to develop future AF promotion programs.
Agronomic and yield selection of doubled haploid lines of rainfed lowland rice in advanced yield trials
Authors: Akbar Miftahur Rizqi , Purwoko Bambang Sapta , DEWI ISWARI SARASWATI , SUWARNO WILLY BAYUARDI , SUGIYANTA SUGIYANTA , ANSHORI MUHAMMAD FUAD
Published: August 2021

Abstract. Akbar MR, Purwoko BS, Dewi IS, Suwarno WB, Sugiyanta, Anshori MF. 2021. Agronomic and yield selection of doubled haploid lines of rainfed lowland rice in advanced yield trials. Biodiversitas 22: 3006-3012. Selection process is important step to obtain high yielding variety. This study aimed to obtain information on agronomic performance of doubled haploid lines of rainfed rice in advanced yield trials and select the best lines for multi-location yield trials. Two experiments were conducted in Bogor and Subang using a randomized complete block design for each location and consisted of 35 doubled haploid lines and two check varieties namely Ciherang and Inpari 18 as a treatment. The result revealed that interaction between genotype and environment (G x E) significantly affected all agronomic characters, except on the panicle length. Characters with high heritability value were all agronomic characters except number of productive tillers and productivity. Genotypic correlation analysis showed that number of productive tillers and weight of 1000 grains had significant and positive correlation with productivity. The selection used weighted selection index based on multivariate analysis. Fourteen lines were selected for multi-location yield trials. These lines had characteristics as follows: number of productive tillers (14.0-17.7 tillers), weight of 1000 grains (24.7-32.5 g), and productivity (5.0-6.2 ton ha-1). The selected lines can be evaluated further to obtain superior lines in multi-location trials.
Diversity, traditional uses and conservation status of Zingiberaceae in Udorn Thani Province, Thailand
Authors: Saensouk Piyaporn , Saensouk Surapon
Published: August 2021

Abstract. Saensouk P, Saensouk S. 2021. Diversity, traditional uses and conservation status of Zingiberaceae in Udorn Thani Province, Thailand. Biodiversitas 22: 3083-3097. Southeast Asia is recognized as the center of Zingiberaceae distribution with Thailand is among the important regions. Nonetheless, a comprehensive study in a regional context that investigates the biological aspects of the family is lacking. This study aimed to determine the diversity, distribution, ecology, conservation status, and traditional uses of the family Zingiberaceae in Udorn Thani Province, northeastern Thailand. In total, three tribes, nine genera, 47 species of Zingiberaceae were identified during a botanical survey between January and December 2020 in Udorn Thani. Curcuma and Kempferia were the most diverse genera with nine and eight species, respectively, followed by Zingiber as the third most diverse genus with seven species and Alpinia as the fourth most diverse genus with six species. While the genera Etlingera and Hedychium were the least diverse with each containing just one species. For all Zingiberaceae species, the flowering period was found between March and September, while the fruiting period was found between May and October. Zingiberaceae was found with the greatest frequency in five ecosystem types, namely cultivated areas, deciduous dipterocarp forest, mixed deciduous forest, dry evergreen forest and river basin. Twenty-one species were reported as rare species in research area, i.e. Boesenbergia baimaii, B. isanensis, Kaempferia picheansoonthonii, and K. udonensis. It was revealed that the traditional uses of many Zingiberaceae species from villagers in Udorn Thani Province were most frequently used for medicine, food, ornamentals, rituals, spices, perfume and cosmetics. Rhizomes, roots, pseudostems, young inflorescences, inflorescences, young leaves, leaves and fruits were the parts of this plant used.
Modeling potential distribution of Baccaurea macrocarpa in South Kalimantan, Indonesia
Authors: Gunawan Gunawan , Rizki Muhammad Ikhwan , Anafarida Olivia , Mahmudah Nurul
Published: August 2021

Abstract. Gunawan, Rizki MI, Anafarida O, Mahmudah N. 2021. Modeling potential distribution of Baccaurea macrocarpa in South Kalimantan, Indonesia. Biodiversitas 22: 3230-3236. Baccaurea macrocarpa is fruit tree that has a potential source of food and medicine. However, little is known about the occurrences and potential distribution of B. macrocarpa mainly in South Kalimantan. This study is the first to predict the distribution of B. macrocarpa in South Kalimantan using maximum entropy (MaxEnt). Modeling included 102 occurrence records with 19 bioclimatic variables, solar radiation, altitude, and slope. Temperature, solar radiation, and precipitation were the key environmental factor influencing the distribution of B. macrocarpa. The district of HS (Hulu Sungai Selatan), HT (Hulu Sungai Tengah), HU (Hulu Sungai Utara), BL (Balangan), and TG (Tabalong) were predicted as highly suitable areas (IHS 0.6-1) for this species. The MaxEnt model performed better than the random method with an Area Under Curve (AUC) value of 0.817, indicating that the model is good and informative model for habitat suitability of B. macrocarpa. The predicted model we presented here can help habitat conservation, biodiversity conservation planning and monitoring, and cultivation in the future for B. macrocarpa.
Effect of three composts with active ingredients of Pseudomonas fluorescens on the development of white root disease and production of rubber plants
Authors: DAMIRI NURHAYATI , ROFIQI RIZAL , MULAWARMAN MULAWARMAN , RAHIM SUPLI E. , FEBYANTI TRI RAPANI
Published: August 2021

Abstract. Damiri N, Rofiqi R, Mulawarnam, Rahim SE, Febyanti TP. 2021. Effect of three composts with active ingredients of Pseudomonas fluorescens on the development of white root disease and production of rubber plants. Biodiversitas 22: 3237-3242. White root disease (WRD) caused by Rigidoporus lignosus is a very dangerous disease and a scourge for rubber farmers because it can result in decreased production and kill rubber plants. This research was conducted to observe the impact of compost enriched with the biological agent Pseudomonas fluorescens on the development of white root disease and production in rubber plants. The results showed that the application of compost with active ingredient of P. fluorescens isolates A and B reduced the severity of white root disease in plants with mild, moderate and severe infections, 34.12%, 29.31% and 57.21% respectively. Application of compost with P. fluorescens isolates A and B, either singly or in combination, can increase latex production. The treatment of giving compost enriched with P. fluorescens isolates AR and ABR on rubber plants infected with mild WRD resulted in the highest latex production of 406 gm and 402.74 gm per plant, respectively. These two treatments did not differ from each other but were significantly different from the other treatments and controls.
The physicochemical composition of honey from Indonesian stingless bee (Tetragonula laeviceps)
Authors: Agussalim Agussalim , Umami Nafiatul , Nurliyani Nurliyani , Agus Ali
Published: August 2021

Abstract. Agussalim, Umami N, Nurliyani, Agus A. 2021. The physicochemical composition of honey from Indonesian stingless bee (Tetragonula laeviceps). Biodiversitas 22: 3257-3263. The demand of honey has recently increased significantly, but this situation is widely used by irresponsible humans who made a fake honey. This present study aims to evaluate the quality based on the physicochemical composition of honey from Indonesian stingless bee Tetragonula laeviceps. Honey was obtained from three geographical origins in Indonesia, i.e. Sleman, Gunungkidul, and Lombok. The physicochemical composition of honey was analyzed such as moisture, ash, protein, pH, glucose/moisture ratio, and electrical conductivity, and amino acids. The results showed that the geographical origins had a highly significant effect (P<0.01) on moisture, pH, and significant effect (P<0.05) on ash, electrical conductivity, protein, and glucose/moisture ratio. Honey from Indonesian stingless bee T. laeviceps was found 17 amino acids were arginine, histidine, lysine, phenylalanine, isoleucine, leucine, methionine, valine, threonine, tyrosine, proline, glutamic acid, aspartic acid, serine, alanine, glycine, and cysteine. The dominant amino acids of honey from Sleman were glutamic acid, histidine, lysine, and arginine. Honey from Lombok were glutamic acid, aspartic acid, lysine, and histidine. Furthermore, honey from Gunungkidul were lysine, arginine, histidine, and phenylalanine. Honey from Sleman and Lombok have the best quality than honey from Gunungkidul based on the chemical composition and amino acids profile.
Pig (Sus scrofa domesticus) dung and their associated fungi: Potential candidates for bio-remediation and nutrients improvement of crude oil contaminated soils
Authors: Enyiukwu David Nwazuo , BASSEY INENESIT N. , NWAOGU GRACE AMARACHI , ASAMUDO N.U. , CHUKWU LWANGA AZUBUIKE , MONDAY E.A. , MARANZU JACQUELINE O.
Published: August 2021

Abstract. Enyiukwu DN, Bassey IN, Nwaogu GA, Asamudo NU, Chukwu LA, Monday EA, Maranzu JO. 2021. Pig (Sus scrofa domesticus) dung and their associated fungi: Potential candidates for bio-remediation and nutrients improvement of crude oil contaminated soils. Biodiversitas 22: 3276-3286. Pig dung a common agro-waste material in Nigeria is associated with many fungal saprophytes.  Rising oil spillages and pollution in Nigeria have made cultivation of affected agro-lands un-rewarding. Potentials of pig dung in mitigating crude oil pollution in agro-soils are herein studied. The objectives of this study were to characterize the fungal profile associated with wet and dry dung of pig. And to evaluate the remediating impacts of pig dung and their associated fungi on crude oil and heavy metals contents, physicochemical properties, and phytotoxicity of crude oil polluted soils. Coprophilous fungi were isolated from wet and dry dung by moist chamber technique and identified. Utisoil from Umudike was polluted with crude oil, amended with 10-40% of dry pig dung and sown with soybean (Glycine max L.). The amended and un-amended crude oil contaminated utisols were analyzed to reduce crude oil and heavy metals contents; and physicochemical properties at planting, and harvesting, respectively, using classical and spectrometric methods, and the extent of crude oil and heavy metals removal determined. The percentage germination, height, number of leaves, leaf color, vigor, and biomass of the test crop were recorded from the test plant. The results showed that Aspergillus candidus, Sclerotium sp., Absidia sp., Acremonium sp., Candida tropicalis, C. pseudotropicalis, and Rhizopus oryzae were consistently isolated from the dry dung specimen than the wet one. The results also indicated that the treatments significantly reduced the treated soils' crude oil and heavy metals contents. It revealed that values obtained for residual oil or heavy metals contents of the treated soils and crop performance were inversely and directly related to levels of soil amendment respectively. Crop performance in the amended soil remained statistically (P?0.05) higher compared to the control. Soil analyses showed that the dung treatments significantly increased the values of nitrogen, phosphorus, potassium, organic carbon, etc. in a directly proportional manner. Therefore, pig dung and their associated fungi hold strong potentials as cheap eco-friendly platforms to provide and stimulate microbial growth, hasten degradation and removal of crude oil and heavy metals from contaminated soils; and thus permit and sustain good performance of arable crops.
Analysis of growth and nutritional quality of sea worms (Nereis virens) as a mass cultured natural feed on different substrate media thicknesses
Authors: Herawati Vivi Endar , Elfitasari Tita , Rismaningsih Nurmanita , Har Riyadi Putut , Tarangkoon Woraporn , Radjasa Ocky , Windarto Seto
Published: August 2021

Abstract. Herwati VE, Elfitasari T, Rismaningsih N, Riyadi PH, Tarangkoon W, Radjasa OK, Windarto S. 2021. Analysis of growth and nutritional quality of sea worms (Nereis virens) as a mass cultured natural feed on different substrate media thicknesses. Biodiversitas 22: 3299-3305. Sea worms (Nereis virens) grow slowly. The thickness of the substrate used in mass culture affects their growth, because N. virens is a deposit feeder that feeds on organic matter provided by other organisms; therefore, the growth rate slows down if the substrate thickness is not suitable. This study aimed to determine and analyze the growth and nutritional quality of N. virens as a natural feed in mass culture at different substrate media thicknesses. This study was conducted at the Marine Science Techno Park (MSTP) UNDIP, Jepara, Central Java. The test animals were two-month-old sea worms, weighing 0.15 to 0.5 g/individual. The maintenance media was the mangrove mud substrate. Feeding was carried out at a fixed feeding rate, twice a day at 07.00 and 19.00 hrs, and the animals were maintained at a density of 45 individuals/L for 35 days. The experimental research method was a completely randomized design with four treatments and three replications: treatment A (media thickness of 4 cm), B (media thickness of 6 cm), C (media thickness of 8 cm), and D (media thickness of 10 cm). The parameters studied were absolute growth, specific growth rate (SGR), efficiency of feed utilization (EFU), feed conversion ratio (FCR), protein efficiency ratio (PER), survival rate (SR), proximate analysis, profile of amino acid and fatty acids. The results showed that the highest absolute weight (6.76 g); SGR (6.34%); EFU (69.73%); FCR (1.36%); PER (1.74%); SR (97.04%); and nutritional quality (55.75% protein, 22.62% fat, 45.66 ppm methionine, and 7.7% eicosapentaenoic acid values were obtained when N. virens was mass-cultured in a medium with a thickness of 8 cm (Treatment C). Thus, the thickness of the media substrate had a significant effect (P < 0.05) on the absolute weight, SGR, EFU, FCR, PER, and SR in N. virens.
Effectivity of bulrush (Scirpus californicus.) as a soil conditioner in increasing sandy and clay soil fertility
Authors: Izzati Munifatul , HARYANTI SRI , HASTUTI RINI BUDI
Published: August 2021

Abstract. Izzati M, Haryanti S, Hastuti RB. 2021. Effectivity of bulrush (Scirpus californicus) as a soil conditioner  increasing sandy and clay soil fertility. Biodiversitas 22: 3423-3429. Bullrush (Scirpus californicus) is a species of macrophytes that often populates lakesides. This aquatic plant was rarely studied and its use has not been explored yet. This study was carried out to determine the effects of soil conditioner made from bulrush on sandy and clay soil fertility. Collected bulrush from Rawa Pening lake was milled into a powder and used as a soil conditioner in a proportion of 1:1. After a week, soil fertility was evaluated including organic matter content, water retention, the ratio of C/N, and bacteria population. The study was designed using a Completely Randomized Design with two treatments and control. Resulted data were analyzed using a t-test to evaluate the difference between the two treatments. Results showed that bulrush powder application significantly increased sandy and clay soil fertility. The organic matter content significantly increased in both sandy (p<0,01) and clay soils (p<005). The water retention of sandy soil was increased by 74% (p<0,01), while in clay soil was reduced by 27% (p<0,01). The C/N ratio was significantly reduced in both sandy and clay soil (p<0,05), while the bacteria population significantly increased (p<0,01). It is suggested to use the bulrush as a soil conditioner particularly for sandy and clay soils.
Flower morphometry of members of the genus Dactylorhiza Necker ex Nevski (Orchidaceae) from the Altai Mountains of Kazakhstan
Authors: Sumbembayev Aidar , ABUGALIEVA SAULE , DANILOVA ALEVTINA , MATVEYEVA EKATERINA , SZLACHETKO DARIUSZ
Published: August 2021

Abstract. Sumbembayev AA, Abugalieva SI, Danilova AN, Matveyeva EV, Szlachetko DL. 2021. Flower morphometry of members of the genus Dactylorhiza Necker ex Nevski (Orchidaceae) from the Altai mountains of Kazakhstan. Biodiversitas 22: 3545-3555. Several species of Dactylorhiza (Orchidaceae) from the Altai mountains of Kazakhstan have been investigated regarding their morphological flower variability. Significant metric characters were identified allowing to differentiate between the four species: D. incarnata, D. fuchsii, D. maculata and D. salina. The morphometric structure of flowers was analyzed by comparing 17 metric parameters in representatives of 11 populations. We identified the most variable and stable traits as well as distinctive features for each species. A high degree of flower morphometric diversity was revealed from principal component analysis for species and populations. Cluster analysis demonstrated the structure of the population diversity. Structural schemes have been compiled from data of the photographic processing of flower morphometry, the analysis of variance ANOVA, and the degree of variation at the population level. Useful characters are provided for further taxonomic work on members of the genus Dactylorhiza in Kazakhstan.
Novel Use of the Muscle Sparing Anterolateral Approach for Septic Arthritis of the Native Hip Joint in an Adult: A Case Report
Authors: Zale Connor , Deal Jr. J Banks , Vaslow Aaron , Lause Gregory , Providence Bertram
Published: August 2021

Introduction: Septic arthritis of a native hip is a relatively uncommon condition in adults. Prompt diagnosis and treatment of septic hip arthritis are imperative to preserve joint integrity, as failure to quickly intervene can allow rapid degenerative changes.
Case Report: This is a case report of a 50-year-old male that presented with right septic hip arthritis in the acute setting. He complained of pain with attempted range of motion and log-roll. Laboratory workup demonstrated no leukocytosis, but elevated inflammatory markers. A hip aspiration under fluoroscopy was performed, revealing a cell count of 100,600/cm3 with 91% neutrophils. His right hip underwent irrigation and debridement with a muscle-sparing anterolateral approach (Rottinger).
Conclusion: This case report describes the successful use of the anterior based muscle-sparing approach for incision and drainage of septic arthritis of the native hip joint in an adult patient. Advantages to this strategy include lower rate of nerve injury compared to classical anterior and lateral approaches, less muscle damage than the posterior approach, and no requirement for special skills or equipment as in the case of hip arthroscopy.
Keywords: Septic arthritis, anterior based muscle sparing and anterolateral
The Neck of Femur Fracture in an Elderly Patient with Schizophrenia and Parkinsonism Managed with Dual Mobility Total Hip Replacement – A Rare Case Report
Authors: Mahajan Neetin P. , Gadod Lalkar Laxman , Chandanwale Ajay S. , Kumar G. S Prasanna , Narvekar Mrugank , Kamble Mayur
Published: August 2021

Introduction: Neck of femur fractures is the most common fractures in elderly people as they present with osteoporosis. There is a higher chance of proximal femur fracture in a schizophrenic patient as there is a loss of bone mineral density due to a drug-induced increase in the level of prolactin and immobility. Patients with Parkinson’s disease were at higher risk for the development of hip fractures due to gait disturbances and instability.
Case Report: A 63-year-old male patient presented with complaints of the right arm pain, swelling, deformity of the right arm, and difficulty in extending the wrist for 3 days. The patient had a history of falls and trauma to the left arm at home. X-ray right arm showed distal one-third spiral humerus fracture for which intramedullary nailing was done. X-ray of the pelvis with both hips showed right neck of femur fracture with associated greater trochanter (GT) fracture. We managed with total hip replacement using a dual mobility cup and tension band wiring for GT fracture. Postoperatively, the patient has a good range of motion at the hip. The functional outcome is good as per Harris’s hip score.
Conclusion: Neck femur fractures in elderly patients with associated comorbidities such as schizophrenia and parkinsonism are best managed with total hip replacement using a dual mobility cup. It avoids the risk of dislocation in high-risk patients and prevents secondary procedures. Careful clinical and radiological examination of the pelvis is very essential even in asymptomatic patients with a history of trauma to prevent delayed diagnosis of these types of fractures in patients with schizophrenia and parkinsonism.
Keywords: Neck of femur fracture, Parkinsonism, schizophrenia, dual mobility cup, total hip replacement.
Severe Kyphotic Deformity Involving Thoracolumbar Region – A Management Dilemma
Authors: Rathod Tushar , Marathe Nandan , Shende Chetan , Jogani Abhinav , Sathe Ashwin , Mallepally Abhinandan Reddy
Published: August 2021

Introduction: Thoracolumbar kyphosis is common cause of sagittal spinal malalignment. Neglected tuberculous infection in childhood often presents as kyphotic deformity in adulthood, especially in India, where tuberculosis is endemic. Complete clinical and radiological evaluation is necessary to decide need for surgical intervention. We came across this case of severe thoracolumbar kyphosis extending into lower lumbar spine due to block vertebra formation from fusion of T12 to L4 vertebrae.
Case Report: The patient presented with debilitating mechanical low back pain without any neurological abnormality in lower limb. The patient was treated surgically with posterior corrective three-column osteotomy and fixation in view of the persistent mechanical low back pain. At 1-year follow-up, the patient showed significant improvement in low back disability score.
Conclusion: After performing three-column osteotomy, we obtained 63% correction in thoracolumbar kyphosis resulting in relief of symptomatic severe axial low back pain and ambulatory capacity with Oswestry Disability Score of 10%. This case highlights long-term natural history of neglected post-tuberculous affection of thoracolumbar region of vertebral column and management challenges of severe thoracolumbar kyphosis.
Keywords: Kyphosis, thoracolumbar, spinal osteotomy.