bluuebunny/crossref_metadata_2025 huggingface dataset:
A Powerful and Universal Preimplantation Genetic Diagnosis Protocol for Cystic Fibrosis
Authors: Viart Victoria , Ishmukhametova Aliya , Plaza Stéphanie , Verrière Garance , Saguet Florielle , Claustres Mireille , Girardet Anne
Published: January 2023

Background: Cystic fibrosis (CF) is one of the most common indications of preimplantation genetic diagnosis (PGD) for monogenic disorders worldwide.

Aims: The aim of this article was to report a universal and powerful assay easily applicable to all couples requesting PGD for CF irrespective of the CFTR variants involved, in line with recently published CF-PGD guidelines.

Results: A multiplex PCR protocol was developed including the study of the c.1521_1523del mutation with 12 closely linked polymorphic markers. Preliminary workup was performed for 53 couples and the protocol was clinically applied to 31 couples. All couples were informative for 7–12 markers. Of the 31 couples who initiated a PGD stimulation cycle, 17 couples had a baby. Therefore, the take-home baby rate was 60.7% per couple with an embryo transfer (17 out of 28 couples).

Conclusion: This robust, simple, and reliable procedure should allow any couple at risk of transmitting CF to enrol in a PGD programme.
Regenerative Medicine, Disease Modelling, and Drug Discovery in Human Pluripotent Stem Cell-Derived Kidney Tissue
Authors: Gupta Navin , Susa Koichiro , Morizane Ryuji
Published: January 2023

The multitude of research clarifying critical factors in embryonic organ development has been instrumental in human stem cell research. Mammalian organogenesis serves as the archetype for directed differentiation protocols, subdividing the process into a series of distinct intermediate stages that can be chemically induced and monitored for the expression of stage-specific markers. Significant advances over the past few years include established directed differentiation protocols of human embryonic stem cells and human induced pluripotent stem cells (hiPSC) into human kidney organoids in vitro. Human kidney tissue in vitro simulates the in vivo response when subjected to nephrotoxins, providing a novel screening platform during drug discovery to facilitate identification of lead candidates, reduce developmental expenditures, and reduce future rates of drug-induced acute kidney injury. Patient-derived hiPSC, which bear naturally occurring DNA mutations, may allow for modelling of human genetic diseases to enable determination of pathological mechanisms and screening for novel therapeutics. In addition, recent advances in genome editing with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 enable the generation of specific mutations to study genetic disease, with non-mutated lines serving as an ideal isogenic control. The growing population of patients with end-stage kidney disease is a worldwide healthcare problem, with high morbidity and mortality rates, that warrants the discovery of novel forms of renal replacement therapy. Coupling the outlined advances in hiPSC research with innovative bioengineering techniques, such as decellularised kidney and three-dimensional printed scaffolds, may contribute to the development of bioengineered transplantable human kidney tissue as a means of renal replacement therapy.
The Evolution of Assisted Reproductive Technologies: A Modern Approach to Ovarian Stimulation
Authors: Whitehouse Christie
Published: January 2023

In celebration of the 40th anniversary of the first in vitro fertilisation (IVF) baby this year, the symposium focussed on the modern-day approach to ovarian stimulation (OS). Chairperson Prof Fauser welcomed delegates with a look at the key achievements related to OS in the context of assisted reproductive technologies (ART) over the past century. Treatments have evolved from the first crude preparations to the refined gonadotrophin products available for clinical use today.

The theme of personalisation in OS was introduced by Dr Labarta, who looked at how we can use accurate biomarker measurements to assess ovarian reserve, predict ovarian response, and, therefore, personalise treatment accordingly. Of the biomarkers currently available, anti-Müllerian hormone (AMH) has been identified as the best tool for individualised gonadotrophin dosing. AMH can also be used to drive evidence-based decisions in the choice of gonadotrophin treatment. Dr Alper presented results from the MEGASET HR trial, which investigated highly purified human menopausal gonadotrophin (HP-hMG) in patients identified via their AMH levels as potential high responders. Dr Havelock then demonstrated how AMH, along with body weight, has allowed for the development of the first dosing algorithm for tailoring treatment with follitropin delta, which has been validated in randomised controlled trials (RCT). Finally, the symposium closed with Prof Fauser concluding that, using the biomarker AMH, it is now possible to personalise not only the dose of gonadotrophin but also the choice of gonadotrophin treatment, representing important first steps in truly individualising OS.
Overcoming Challenges in Reproductive Health Applications by Deploying More Sensitive and Accurate Molecular Technologies
Authors: Brasch-Andersen Charlotte , Behar Doron M. , Garcia Herrero Sandra , Rubio Carmen
Published: January 2023

The field of reproductive health is progressing rapidly from traditional non-molecular technologies based on visual microscope-based techniques to the latest molecular technologies, that are more accurate, objective, and efficient, and some of which are less invasive. Genome-wide technologies have been applied at different stages of the reproductive health lifecycle, such as preimplantation genetic testing, prenatal and postnatal testing, and preconception carrier screening. Next-generation sequencing is currently the platform of choice when it comes to preimplantation genetic testing, and analysis using cell-free DNA offers a potential non-invasive alternative to current methods. Molecular tests of endometrial receptivity identify the optimum timing for embryo implantation, thereby improving in vitro fertilisation (IVF) success rates for patients with recurrent implantation failure of endometrial origin. In the prenatal and postnatal settings, new technologies, such as microarrays and next-generation sequencing, have increased the diagnostic yield and fuelled the rate of discovery of new genetic syndromes. Expanded carrier screening panels have replaced multiple single-gene tests with a single assay and have been shown to be more effective at identifying carriers of genetic disorders. These innovations are accompanied by new challenges regarding their implementation and use. Patient access to new technologies varies greatly and several factors have been identified as barriers to uptake. Genetic counselling has become increasingly important as the amount of genetic information provided by these technologies continues to rise. This review discusses specific challenges associated with traditional non-molecular and older-generation molecular techniques in reproductive health, and suggests potential solutions provided by recent advances in genetic technologies.
PKM Pendampingan Pengembangan Bakat dalam Meningkatkan Prestasi Siswa SD Islam Miftahul Ulum Pakuniran Probolinggo
Authors: Rifa'i Moh. , Muchyiddin Ach. , Hosen Mohammad , Mabrur Moh. , Burhanuddin Syamsul Aimmah , Muhammad Usamah Bin , Fatoni Moh. Yusni , Asy’ari Asy’ari
Published: January 2023

Peningkatan prestasi siswa sangat urgen. Implikasi prestasi siswa dapat berkontribusi terhadap peningkatan kepercayaan masyarakat terhadap sekolah. Sekian banyak pengelolanya berusaha meningkatkan layanan agar dapat mencetak calon-calon lulusan yang beprestasi. Sekian banyak problematika yang dihadapi termasuk dalam kondisi ketidaksiapan yang maksimal dalam mengikutsertakan siswanya dalam event-event. Maka diperlukan pendampingan maksimal dalam membina bakat siswa yang telah tersedia. Pengabdian ini dilakukan dengan pendekatan PAR (Participatory Action Research) dikuatkan dengan metode survey lapangan, analisis masalah yang berkembang di lapangan, FGD (Focus Group Discussion), analisis SWOT, tindakan langsung di lapangan yang terdiri atas program pendampingan dalam pendampingan pengembangan bakat siswa yang dapat berimplikasi terhadap peningkatan prestasi siswa dan citra lembaga. Objek pendampingan adalah para siswa berbakat di SD Islam Miftahul Ulum. Program PKM pendampingan pengembangan bakat dilakukan melalui program pendampingan pelatihan siswa berbakat sesuai kompetensi masing-masing siswa dengan pengaturan jadwal pendampingan bagi peserta didik berbakat yang dikuatkan dengan pembagian tugas yang dilakukan oleh peserta PKM program studi manajemen pendidikan Islam Fakultas Agama Islam Universitas Nurul Jadid dan di akhir dengan implementasi pendampingan terhadap program promosi siswa berbakat. Pendampingan ini memberikan kontribusi yang efektif dan efisien dalam peningkatan bakat siswa secara berkelanjutan.
PKM Pelatihan Kepemimpinan Dasar untuk Membentuk Jiwa Kepemimpinan yang Responsif dan Inovatif pada Siswa Kelas Akhir di Sekolah Menengah Atas (SMA) Nurul Jadid Paiton Probolinggo
Authors: Abdurrahman Abdurrahman , Surianto Surianto , Marsuki Moh , Ainul Fatah Muhammad Arifin , Arifin Syamsul , Ansyah Nurdi , Sa’dullah Masrur Lu’ai
Published: January 2023

SMA Nurul Jadid merupakan salah satu lembaga pendidikan yang berada di bawah naungan SMA Nurul Jadid Pondok Pesantren Nurul Jadid dan telah berdiri sejak tahun 1973 dengan jumlah siswa 1352 di tahun ajaran 2021-2022 dengan rincian 350 untuk kelas akhir (XII), 433 kelas XI dan sisanya kelas X. Dari 350 siswa kelas akhir, besar kemungkinan 70 hingga 80% di antaranya lebih memilih masuk di perguruan tinggi di luar Pondok Pesantren Nurul Jadid. Keadaan ini menjadi peluang sekaligus tantangan bagi mereka untuk mempersiapkan diri menghadapi dunia baru yang lebih kompleks. Meski demikian, permasalahhan yang dihadapi SMA Nurul Jadid adalah kurangnya tenaga ahli dan profesional dalam pendidikan kepemiminan. Untuk itu, siswa kelas akhir tersebut perlu dibekali dengan beberapa keahlian, salah satunya adalah kepemimpinan. Pelatihan kepimpinan dasar tersebut diharapkan menjadi bekal ketika mereka sudah terjun di dunia kampus. Tema pengabdian ini adalah pelatihan yang terdiri dari sosialisasi, pelatihan dan penilaian/evaluasi. Diharapkan pelatihan kepemimpinan dasar ini dapat menjadi strategi dalam mewujudkan generasi yang berjiwa pemimpin yang responsif dan inovatif ketika sudah berkecimpung di dunia organisasi, baik di dalam maupun di luar kampus
PKM Family Centered Care sebagai Solusi Keamanan Finansial dan Pencegahan Amputasi pada Penderita Luka Ganggren di Paiton Kabupaten Probolinggo
Authors: Nugroho Setiyo Adi , Pratidina Amanda Virga , Widad Sofil , Rizqiyah Afifatur , Hasanah Nurul , Safitri Rike Nur
Published: January 2023

Penderita Diabetes Melitus sangatlah beresiko mengalami luka _ontrol_ yang buruk. Gangguan finansial terjadi akibat biaya perawatan luka yang sangat mahal. Beban finansial membuat banyak penderita DM tidak merawat luka dengan baik sehingga jatuh kepada amputasi bahkan kematian. Program Kemitraan Masyarakat (PKM) dilakukan untuk mendorong kemandirian keluarga penderita DM, target PKM ini menciptakan Family Centred Care yang dimana dapat menghemat sampai dengan 75% pengeluaran biaya perawatan luka dan tentunya mencegah terjadinya amputasi. Keluarga Mitra diberikan pelatihan perawatan luka modern, selain itu keluarga mitra mendapatkan pendampingan selama program. Tim PKM melakukan kunjungan setiap minggu sebagai _ontrol dari perawatan luka. Hasil dari PKM selam selama 2 Bulan (April-Mei) yang melibatkan 10 orang mitra membuat Family Centred Care tercapai, mitra dapat menghemat finansial sampai 75%, pekembangan luka yang sangat baik. Untuk itu, tenaga kesehatan diharapkan dapat memberikan pelatihan dan pendampingan perawatan luka secara modern kepada keluarga penderita DM.  Family Centred Care menjadi solusi terbaik mengatasi masalah finasial penderita DM
PKM Pelatihan Penggunaan Sistem Informasi Desa Berbasis Digital kepada Perangkat Desa Mengen Kecamatan Tamanan Bondowoso
Authors: Wijaya Andi , Dewi Sinta , Amalia Sholehatul , Khoiriyah Septia Anis , Singgih Nur Muhammad Ian Bima
Published: January 2023

Kemampuan literasi digital perangkat desa merupakan aspek yang paling utama dalam mendukung proses pelayanan kepada masyarakat. Aplikasi sistem informasi desa yang dikembangkan secara mandiri oleh Pemerintah Desa Mengen Kecamatan Tamanan dan Fakultas Teknik Universitas Nurul Jadid, hal ini karena perangkat desa belum memahami penggunaan Sistem Informasi Desa. Pelatihan penggunaan sistem informasi desa Berbasis Digital Kepada Perangkat Desa Mengen Kecamatan Tamanan dibutuhkan oleh perangkat desa untuk meningkatkan kemampuan literasi digital perangkat desa. Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan kemampuan literasi digital perangkat Desa Mengen Kecamatan Tamanan yang merupakan desa binaan Fakultas Teknik Universitas Nurul Jadid. Metode yang digunakan adalah dengan metode pelatihan dan tutorial yang dilakukan dengan 6 tahapan, yaitu 1) Studi Pendahuluan, 2) Penyusunan Rencana, 3) Identifikasi, 4) Pelaksanaan, 5) Pendampingan dan 6) Evaluasi. Hasil dari pelatihan Pelatihan Penggunaan Sistem Informasi Desa Berbasis Digital Kepada Perangkat Desa Mengen Kecamatan Tamanan menghasilkan peningkatan keterampilan dan pengetahuan perangkat desa setelah mengikuti seluruh rangkaian materi yang diberikan
PKM Peningkatan Edukasi Ekonomi Kreatif melalui UMKM Kampong Snack dalam Mewujudkan Kemandirian Ekonomi Masyarakat di Kabupaten Probolinggo
Authors: Fathony Alvan
Published: January 2023

Potensi lokal merupakan suatu aset nasional yang jika dimaanfaatkan secara maksimal dapat menambah pendapatan nasional untuk kesejahteraan rakyat. Namun, masyarakat Indonesia masih didominasi oleh kalangan yang secara praktis masih berada pada garis kemiskinan. Data Badan Pusat Statistik menunjukkan angka kemiskinan meningkat hingga 28,95 juta atau mencapai 12.25 persen dari total penduduk di Indonesia. Salah satu penyebabnya, yaitu kurangnya sosialisasi dan penerapan pendidikan ekonomi kreatif di lingkungan masyarakat yang berada di wilayah pelosok, seperti Kabupaten Probolinggo di Provinsi Jawa Timur yang memiliki beragam potensi lokal diberbagai sektor UMKM. UMKM merupakan produk sumber penunjang ekonomi bagi masyarakat Desa Alastengah. Ironisnya, selama terbentuknya UMKM ini, hasil penjualan selalu mengalami penurunan, maka diperlukan sebuah inovasi kreatif dan inovatif bagi masyarakat setempat. Oleh karena itu, tim menggagas program Pengabdian Masyarakat dengan judul “Peningkatan Edukasi Ekonomi Kreatif Melalui UMKM Kampoeng Snack guna mewujudkan kemandirian ekonomi masyarakat”, sebuah program yang dapat membentuk masyarakat yang memiliki jiwa entrepreneurship dan kompetitif dengan pemberian edukasi terkait ekonomi kreatif. Sehingga tidak hanya terbatas pada strategi pemasaran lewat E-Commerce saja. Hal ini bertujuan untuk membentuk daya saing masyarakat Alastengah dalam memasarkan produk UMKM Kampoeng Snack ini
PKM Pelatihan serta Pendampingan Perangkat Pembelajaran Berbasis Digital sebagai Alat Guru pada Era Digital di SDN Sukodadi 2 Probolinggo
Authors: Fajri Fathorazi Nur , Rahman Abd , Rahman Afiqur , Permatasari Uky Oktavia Risti , Hasanah Uswatun , Sholehatin Dewi , Sugianti Indri Ratna , Musfiroh Musfiroh
Published: January 2023

Pada era digital saat ini, di mana proses pembelajaran (interaksi) berlangsung mulai bergeser menjadi interaksi pembelajaran digital. Sehingga guru perlu menggunakan strategi pembelajaran salah satunya dengan penggunaan perangkat pembelajaran berbasis digital. Tujuan kegiatan pengabdian ini untuk pendampingan dalam membuat video pembelajaran dengan menggunakan Microsoft Power Point pada guru pengajar di SDN sukodadi 2. Sedangkan hasil dari kegiatan ini adalah 1) Guru mengerti cara membuat video pembelajaran. 2) Guru dapat menggunakan video pembelajaran tersebut dalam proses mengajar. 3) Peserta didik mendapatkan kemudahan dan pengalaman belajar yang menyenangkan
PKM Pelatihan Merawat Jenazah pada Siswa MI Tarbiyatul Wathan Sidomukti Kraksaan Probolinggo
Authors: Mahbubi M , Multazam Muhammad Fadil , Ramadhoni Ash-Shiddiqi
Published: January 2023

Kondisi kesedihan keluarga di daerah yaitu sering mengalami ketidakmampuan dalam pengurusan jenazah. merawat jenazah menjadi fardhu kifayah bagi umat Islam dalam menyelenggarakan pengurusan jenazah dengan kewajiban untuk memandikan, mengkafani, menshalatkan serta menguburkan, karena rangkain prosesi pengurusan jenazah bermakna dalam nilai nilai kehidupan masyarakat. Permasalahan di atas menjadi alasan bagi kami, Tim KKN OBE 2022 UNUJA Prbolinggo untuk membentuk Tim pengabdian kepada masyarakat (PKM) dan mengadakan pelatihan perawatan jenazah sebagai usaha untuk membantu pengetahuan dalam pengurusan penyelenggaraan jenazah.
PENGEMBANGAN KOMPETENSI TEKNOLOGI INFORMASI KOMUNIKASI BAGI APARATUR SIPIL NEGARA DINAS KOMUNIKASI, INFORMATIKA, DAN STATISTIK KABUPATEN BANDUNG
Authors: Putra Ardi Pramudya , Tefa Gradiana
Published: January 2023

The development of Information and ommunication Technology (IT) competencies is an important thing that the Civil Servant (ASN) needs to be able to carry out their duties in the digital era. One of the implementations of digitalization in government organizations is e-government. One of the regional apparatus that is close toimplementing e-government is the Department of Communication, Informatics and Statistic, Bandung Regency. As a leading sector in the field of technology in Bandung Regency, it is necessary for ASN who have competence in the field of ICT. But in fact, the number of employees who have competence in the field of ICT is still minimal so that it makes the authors interested in conducting this research. The purpose of this study was to determine the implementation of ICT competency development for ASN at the Department of Communication, Informatics and Statistic, Bandung Regency throught Hasibuan’s (2007) theory study which consist of 2 dimensions, namely formal development and informal development. This study use a qualitative descriptive method with an inductive approach. Based on the results of the research, it is known that the implementation of formal competency development is carried out throught education and training in collaboration with third parties and requires a budget so that it cannot be implementation evenly. Meanwhile, informal competency development is carried out by means of independent training so that it cannot obtain maximum results compared to throught education and training. Efforts are being made to improve the competence of ASN in the ICT sector by prioritizing the training budget, as well as motivating unskilled employees to improve their competence by learning from more skilled colleagues so as to create a work team that can work together to support organizational performance.

 

Keywords : Competency Development, Information and Communication Technology, Civil Servant
Sustainable Management of Pesticides
Authors: Santosh P. Supanekar , Yojana S. Muniv
Published: January 2023

Pesticide is any substance or mixture of substances intended for preventing, destroying, repelling, or mitigating any pest (insects, mites, nematodes, weeds, rats, etc.), including insecticide, herbicide, fungicide etc. used to control pests and to safeguard public health. Use of pesticide has secured the food production and enhanced the amount of produce. Once the pesticide enters in contact with the environment, its fate is determined by several natural processes (adsorption, degradation, erosion and leaching to groundwater). Of the total pesticides applied in agriculture, about 1-10% reach to the target plant, about 30% is lost in atmosphere, nearly 1-5% is lost due to runoff/surface erosion and roughly 1% is lost by leaching. Pesticides applied in the soil leaches up to 50 -100 %. Pesticide management reduces contamination of water from pesticides and foster effective and safe use of pesticides without causing degradation to the environment. This study provides an overview of pesticides on recent developments in sustainable management on strategies, practices and their effectiveness, core elements, principles of Integrated Pest Management (IPM) and bio-pesticides in crop protection. This study recommends that during application of pesticides, guidelines approved by FAO and WHO (2014), from production to disposal of pesticides in the 'International Code of Conduct on Pesticide Management' (4th version) should be strictly followed by government regulators, the private sector, civil society as a best practice in managing pesticides throughout their lifecycle.
Environmental Impacts of Pesticides - A Review
Authors: Santosh P. Supanekar
Published: January 2023

Pesticides are applied in various sectors such as agriculture, domestic, material building, personal and public health for control of weeds, pests, rodents; household and garden spray, control of animals and birds; incorporation of paints, glues, plastic protection sheeting; for application of clothing and skincare; and for control of malaria, dengue fever and cholera. In agriculture, chemicals are purposefully discharged into the environment. Application of pesticides in agriculture is a subcategory of the largest group of manufactured compounds used in the present world. Excessive and inappropriate use of pesticides has resulted in contamination of environment. Pesticides are degraded into organic micro pollutants that have negative effects on the environment. By the mechanism of biomagnification and bioconcentration, they cause deleterious effects on the living organisms. Ecological impacts of pesticides are different but they are chronic and inter-related. During precipitation, pesticides from land, float to aquatic systems and become harmful to fishes, birds, non-target creatures, etc. They also interact with injurious algal blooms, which enhances the original poisonous nature of the pesticides. This review focuses on environmental impacts of pesticides on contamination of water, fate, effects and behaviour in the environment, effects of contamination on natural resources, food safety, non-target organisms and threats to biodiversity. This study recommends application of suitable amounts of less harmful formulation of pesticides, as and when required.
Karyotypic Studies on Seven Chlorocyphid Species Based on C-Bands, AgNOR’s and AT-GC Regions with Review of the Hitherto Cytologically Examined Data of Family Chlorocyphidae (Odonata: Zygoptera)
Authors: Neha Katnoria , Gurinder Kaur Walia
Published: January 2023

Cytogenetic characterization on seven species of family Chlorocyphidae by carbol-fuchsin staining, Cbanding, silver nitrate staining and DAPI and CMA3 staining has been done. Among these, Aristocypha quadrimaculata, A. trifasciata, Heliocypha biforata, H. bisignata, H. perforate and Paracypha unimaculata are of subfamily Rhinocyphinae and Libellago lineata is of subfamily Libellagininae. All the species of subfamily Rhinocyphinae possess n=12m, which is the type number of the subfamily and complement is characterized by the presence of one large autosomal bivalent, originated by the autosomal fusion. But L. lineata with n= 13m is the only species of subfamily Libellagininae. C-bands and NOR’s are mostly detected on terminal ends of autosomal bivalents in all the 7 species and also at interstitial regions of large bivalent. X chromosome is entirely C-positive in all the species, while it is NOR-positive in both the species of genus Aristocypha, P. unimaculata, H. perforata, and shows NOR on one terminal end in H. bisignata and L. lineata. m bivalent is C-negative in H. biforata and in both the species of genus Aristocypha, while shows C-bands on both the terminals in H. perforata, P. unimaculata and L. lineata, whereas possesses C-band on one terminal end in H. bisignata. m bivalent shows NOR on one terminal end in P. unimaculata and L. lineata, while NOR-negative in all the species of genera Aristocypha and Heliocypha. Complement of all the species shows bright DAPI and weak CMA3.signals which depicts the presence of more AT rich regions than GC regions.
Medicinal Plants Survey in Pudurnadu of Jawadhu Hills Tamil Nadu and Analysis of Phytoconstituents and Antibacterial Activity of Bryophyllum pinnatum Leaves Extract
Authors: Shariq K. Muhammed , Liyakath Ali A.  , Tariq N.P.M. Mohamed , Hussain A. Amjad , Farook M.A.  , Begum V.S. Munasira
Published: January 2023

A plant survey is usually conducted for examining a certain geographic area, analysis of existence of specific species and assessing of habitat appropriateness of those species available in that specific study area. A survey of medicinal plants in a given location helps in the comprehension of overall ecological circumstances by classifying the recorded plants into distinct biological life forms. The current study focuses on survey of ethnomedicinal plants in Pudurnadu. Pudurnadu is a village in Tirupattur district, Tamil Nadu, India, about 54 miles south of Vellore city. One of the effective ways to plant drug discovery has been discovered through ethnomedicinal survey. In our study, we recorded 146 medicinal plant species belonging to 59 families for the treatment of various ailments. The objectives of this study were to identify a rare medicinal plant in our study area and evaluate the phytochemical and antibacterial properties with several extracts of Bryophyllum pinnatum which was determined using the Agar well diffusion method against Gram-positive and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis).
Efficacy of Taditional Yoga on Breath Holding Time and Anxiety Among Middle Aged Women Fireworkers Suffering from Pneumoconiosis
Authors: Srimathi S.  , Muthulakshmi  , Elangovan R.
Published: January 2023

The goal of this random group experimental study was to determine the effect of Yoga therapy on selected psychosocial factors and respiratory parameters in middle-aged women with pneumoconiosis. To achieve the study's aim, 40 middle-aged pneumoconiosis-affected women aged 35 to 50 were randomly selected from fireworks manufacturing factories in Sivakasi, Tamil Nadu. The subjects were split up into two groups of 20 each: experimental and control. It was anticipated that the influences of yoga therapy would lead to significant differences in psychosocial factors and respiratory factors such as anxiety and breath holding time among middle-aged female fire worker suffering from pneumoconiosis. The experimental group received yoga therapy training for 12 weeks (six days per week). The control group was kept in a condition of active rest. Both groups had a pre-test and a posttest before and after the training. Both anxiety and the length of time spent holding one's breath were counted. The data collected from the groups before and after the training session were statistically examined using Analysis of Variance (ANOVA) to identify the significant difference and tested at 0.05 level of confidence. According to the study, yoga therapy reduces anxiety while lengthening breath holds. Therefore, it can be said that yoga therapy assisted middle-aged female fire workders with pneumoconiosis in controlling their anxiety and enhances their breath holding capacity.
THE EFFECT OF REWARDS ON STUDENTS' RESPONSIBILITY IN LEARNING OF SCIENCE OBJECTS AND ITS OBSERVATIONS FOR CLASS VII OF JUNIOR HIGH SCHOOL
Authors: Findy Malita Nisala , Kuswanti Nur
Published: January 2023

In learning science, students must be motivated to have several aspects of learning, including attitudes, so that in the future they can become human beings who have complete potentialis according to educational goals. The purpose of this study was to determine the effect of giving rewards on students’ responsibilities in Science Objects and Their Observations learning of class VII of junior high school. This research used a quasi-experimental design with a post test only control group design. The data were obtained through observations referring to the observation instrumens of students’ attitude.The population used were 320 students of class VII of SMP Negeri 2 Jombang and its samples were 64 students of two classes with 32 people each. The results of the study showed that there were differences of responsibility appearances between students of the experimental and the control classes. In the experimental class, the aspect of responsibility for carrying out the task got an average score of 7.25 and the control class got an average score of 6. For the aspect of responsibility of collecting assignment on time, the experimental class got an average score of 2 and the control class got an average score of 0.9. Their t test showed significant differences between them (p<0,05). Based on these results it can be concluded that reward influenced the students’ attitudes in learning Science Objects and Their Observation material of class VII of SMP Negeri 2 Jombang with higher occurrences in the class being given the reward treatment.
Dynamics of Standardised Quality. Long-term shifts in organic product qualification
Authors: Arnold Nadine , Dombrowski Simon
Published: January 2023

The qualities of standardised products are often perceived as naturally stable. This article scrutinises this perceived stability and investigates which aspects of standardised quality remain stable, and which change in the longer term. Our conceptual framework, anchored in the literature on standards and valuation studies, suggests that while standardised qualities appear to be stable over time and space, it is in these spatial and temporal dimensions of qualification that controversies and changes are expected. Empirically, we investigate the organic quality which has been maintained in the German mass market since the 1970s by the standard-setter Bioland. Searching our archival data for disruption that refers to events, which were interpreted by Bioland as reasons for adjusting the qualification, the data show that Bioland reacted swiftly to manifold disruption triggered by actors located along the production and distribution chain as well as outside it. Pooling Bioland’s responses, we identify four shifts in terms of the (1) meaning, (2) focus, (3) organisation, and (4) relationships of quality. Due to these long-term shifts, little except the name of the standardised quality remained stable. Thus, the article concludes that standardised qualification must be dynamic and changeable if it is to be stably relevant in markets.
Real-World clinical outcomes of asthma patients switched from reslizumab to mepolizumab or benralizumab
Authors: Walsh Laura J. , Casey Deborah , Vairamani Punitha , Arnott Fiona , Plant Barry J. , Murphy Desmond M.
Published: January 2023

Introduction

Approximately 3%–10% of asthma patients will remain uncontrolled despite maximum, optimal conventional therapy. Treatment of severe refractory asthma often involves the use of targeted biological therapy. Randomised controlled trials have shown improvements in clinical parameters with these treatments but real-world data is lacking.

Methods

The clinical parameters, frequency of exacerbations, number of hospital admissions, asthma control questionnaire score (ACQ), forced expiratory volume in one second (FEV1) and maintenance oral corticosteroid (OCS) dose of twenty asthma patients switched from reslizumab to benralizumab or mepolizumab at 1 year prior and 6 months after switching were compared, with adjustments for time.

Results

The mean frequency of exacerbations (0.35 v 0.3) and the mean ACQ were essentially unchanged (1.6 v 1.5) following the switch. The number of hospital admissions was one in the 6 months post switch compared to one in 1-year pre switch. 25% of patients were on maintenance OCS before and after switching but one patient required an increased dose post switch resulting in an increase in the mean maintenance OCS dose (1.6 mg to 2.4 mg). The mean FEV1 was unchanged (80% v 77.9%) six months post switching. Regarding asthma control (n = 19), 47.4% were controlled pre and post switch (ACQ < 1.5), 36.8% remained uncontrolled despite switching, 10.5% improved control while 5.3% disimproved.

Conclusion

We present real-world clinical outcomes of asthma patients switched from reslizumab to either benralizumab or mepolizumab without a loss of clinical effectiveness in the majority.
Role of biochar in anaerobic microbiome enrichment and methane production enhancement during olive mill wastewater biomethanization
Authors: Abid Nozha , Karray Fatma , Kallel Imen , Slim Mariam , Barakat Abdellatif , Mhiri Najla , Chamkha Mohamed , Sayadi Sami
Published: January 2023

The current research work attempted to investigate, for the first time, the impact of biochar addition, on anaerobic digestion of olive mill wastewater with different initial chemical oxygen demand loads in batch cultures (10 g/L, 15 g/L, and 20 g/L). Methane yields were compared by applying one-way analysis of variance (ANOVA) followed by post-hoc Tukey’s analysis. The results demonstrated that adding at 5 g/L biochar to olive mill wastewater with an initial chemical oxygen demand load of 20 g/L increased methane yield by 97.8% and mitigated volatile fatty acid accumulation compared to the control batch. According to the results of microbial community succession revealed by the Illumina amplicon sequencing, biochar supplementation significantly increased diversity of the microbial community and improved the abundance of potential genera involved in direct interspecies electron transfer, including Methanothrix and Methanosarcina. Consequently, biochar can be a promising alternative in terms of the recovery of metabolic activity during anaerobic digestion of olive mill wastewater at a large scale.
Objective circulation classification of rainstorm days associated with Northeast China cold vortexes in the warm seasons of 2000–19
Authors: Huang Lijun , Cui Xiaopeng , Chen Liqiang , Zou Qiangli , Yang Yuting
Published: January 2023

This study conducts objective circulation classifications of rainstorm days associated with Northeast China Cold Vortexes (NECVs) in the northeast of China (NEC) during the warm seasons (May–September). To determine the optimal method and number of types, the performances of ten objective circulation classification methods are first evaluated by several evaluation indexes. Self-Organizing Maps method is then used as the optimal method to classify rainstorms into five types. The results show that the different synoptic circulation patterns are accompanied by distinctive large-scale circulation backgrounds, precipitation characteristics, thermodynamic and moisture conditions. In type 1, the strong western Pacific subtropical high extends north to connect with the mid-latitude ridge in the east of the NEC, and a shallow trough lies in the west of the NEC. This configuration brings the most daily and hourly mean precipitation of all types. A low-pressure anomaly with an obvious trough controls the NEC in type 2, which has a higher frequency. In type 3, the low-pressure anomaly shrinks to the south of the NEC, and the NEC is controlled by the cut-off low vortex. Type 4 has the strongest hourly precipitation and features a meridional high-low-high pressure anomaly, and the narrow zonal low-pressure anomaly is in the NEC. Two low-pressure anomalies and a westerly trough can be found in type 5 and are distributed in a southwest-northeast orientation. These synoptic circulation patterns and the corresponding spatial distribution of rainstorm-day precipitation indicate that the objective circulation classification is effective in helping understand the large-scale circulation and precipitation characteristics associated with NECVs.
MPEFT: A novel task scheduling method for workflows
Authors: Pu Juhua , Meng Qiaolan , Chen Yexuan , Sheng Hao
Published: January 2023

Optimizing the scheduling algorithm is a key problem to improving the service efficiency of urban heterogeneous computing platforms. In this paper, we propose a novel list-based scheduling algorithm called Modified Predict Earliest Finish Time (MPEFT) for heterogeneous computing systems with the aim to minimize the total execution time. The algorithm consists of two stages: task prioritization and processor selection. In the task prioritization phase, the priority of tasks is calculated by time cost of all paths from a task to the exit task. Compared with the prior works, more accurate task priorities are obtained by considering not only the critical path but also the non-critical ones. In the processor selection phase, the processor is allocated for a task according to whether the computing resources are sufficient to its successive tasks. The experiments on randomly generated workflows and the workflows from practical applications show that the MPEFT outperforms other existing list scheduling algorithms.
TRPV4 mRNA is elevated in the caudate nucleus with NPH but not in Alzheimer’s disease
Authors: White Hunter , Webb Ryan , McKnight Ian , Legg Kaitlyn , Lee Chan , Lee Peter H.U. , Spicer Olivia Smith , Shim Joon W.
Published: January 2023

Symptoms of normal pressure hydrocephalus (NPH) and Alzheimer’s disease (AD) are somewhat similar, and it is common to misdiagnose these two conditions. Although there are fluid markers detectable in humans with NPH and AD, determining which biomarker is optimal in representing genetic characteristics consistent throughout species is poorly understood. Here, we hypothesize that NPH can be differentiated from AD with mRNA biomarkers of unvaried proximity to telomeres. We examined human caudate nucleus tissue samples for the expression of transient receptor potential cation channel subfamily V member 4 (TRPV4) and amyloid precursor protein (APP). Using the genome data viewer, we analyzed the mutability of TRPV4 and other genes in mice, rats, and humans through matching nucleotides of six genes of interest and one house keeping gene with two factors associated with high mutation rate: 1) proximity to telomeres or 2) high adenine and thymine (A + T) content. We found that TRPV4 and microtubule associated protein tau (MAPT) mRNA were elevated in NPH. In AD, mRNA expression of TRPV4 was unaltered unlike APP and other genes. In mice, rats, and humans, the nucleotide size of TRPV4 did not vary, while in other genes, the sizes were inconsistent. Proximity to telomeres in TRPV4 was <50 Mb across species. Our analyses reveal that TRPV4 gene size and mutability are conserved across three species, suggesting that TRPV4 can be a potential link in the pathophysiology of chronic hydrocephalus in aged humans (>65 years) and laboratory rodents at comparable ages.
Analysis of cranial ultrasound images for newborn
Authors: Zhang Qing , Zhou Xihui
Published: January 2023

Introduction

Neonatal cranial ultrasound plays an important role in the evaluation of neonatal brain development and related diseases.

Methods

This paper preliminarily explored the analysis and interpretation methods of neonatal brain ultrasound images, and applied the relevant medical image analysis methods to analyze the relevant neonatal brain ultrasound images in more detail.

Results

Compared with other types of imaging methods, ultrasound has its unique advantages and characteristics in such applications as neonatal head imaging.

Discussion

The analysis steps and schemes adopted in this paper have certain reference significance for the analysis of other neonatal brain pictures.
Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity
Authors: Cai Jinlin , Liang Zijian , Li Jian , Manzoor Muhammad Faisal , Liu Hongsheng , Han Zhong , Zeng Xinan
Published: January 2023

Introduction

Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.

Objective

The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree – 1, 2, 3, 4, and 5).

Methods

The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays. Ion chromatography system was used to analyze the monosaccharide composition, and the molecular weight was measured by HPGPC. The polysaccharides were also analyzed by FT-IR and their radical scavenging effect against DPPH, hydroxyl radicals and ABTS was evaluated. The UV-vis assay and gel electrophoresis assay were performed to investigate the DNA damage protective effect.

Results

Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides. However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics. Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals. The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight. Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA. The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart. Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability.

Conclusion

Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction. This study provided valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications.
Anxieties, age and motivation influence physical activity in patients with myeloproliferative neoplasms - a multicenter survey from the East German study group for hematology and oncology (OSHO #97)
Authors: Felser Sabine , Rogahn Julia , le Coutre Philipp , Al-Ali Haifa Kathrin , Schulze Susann , Muegge Lars-Olof , Gruen Julia , Geissler Jan , Kraze-Kliebhahn Veronika , Junghanss Christian
Published: January 2023

Background

Physical activity (PA) is a non-pharmacological approach to alleviate symptom burden and improve health-related quality of life (HrQoL) in cancer patients (pts). Whether pts with myeloproliferative neoplasms (MPN) PA behavior changes due to symptom burden and/or knowledge of the putative beneficial effects of PA has not yet been investigated.

Methods

We performed a large questionnaire study in MPN pts. Self-reported PA behavior and potential influencing factors of 634 MPN pts were analyzed. Questionnaires were used to assess demographics, anxiety, severity of symptoms, HrQoL, current level of everyday and sports activities, and the level of information regarding the importance/possibilities of PA. According to their PA, the pts were assigned to the three groups: “inactive”, “non-targeted active”, and “sporty active” and compared with each other.

Results

Key findings are that in 73% of the pts, the disease had an impact on PA, with 30% of pts reducing their PA. The prevalence of anxieties (e.g., occurrence of thrombosis and bleeding) regarding PA was 45%. Sporty active pts had a lower symptom burden and better HrQoL (p ≤ 0.001) compared to the other groups. Inactive pts were significantly older and had a higher body mass index than sporty active pts. Inactive and non-targeted active pts felt less informed about the importance/possibilities of PA (p = 0.002).

Conclusion

Our results suggest that especially older and non-sporty MPN pts could benefit from motivational as well as disease-specific PA information. This study was registered at the German Registry of Clinical Trials, DRKS00023698.
Study on anxiety and depression of men who have sex with men: An application of group-based trajectory model
Authors: Wu Dan , Zhong Xiaoni , Deng Ruibin , Pan Hong , Gao Yuwen , Lin Bing , Tang Xian , Dai Jianghong , Liang Hao , Huang Ailong
Published: January 2023

The prevalence of anxiety and depression is high among men who have sex with men (MSM), but limited studies focus on their development trends. This study examined the prevalence and influencing factors of anxiety and depression in HIV-negative MSM. In this study, 711 subjects were followed up every 24 weeks for 96 weeks. A group-based trajectory model was used to identify different development trends and a logistic regression model was used to explore the influencing factors of different trajectories. Low, moderate, and high anxiety groups accounted for 32.56, 56.12, and 11.32%; and low and high depression groups accounted for 73.90 and 26.10%. High anxiety was associated with a lower education degree, little HIV-related knowledge, and other characteristics (more agreed that “HIV is a threat to me/my family,” had high frequency of seeking sexual partners via the Internet, had a history of sexually transmitted disease (STD), and a sense of discrimination by doctors). High depression was associated with a lower education degree, little HIV-related knowledge, being bisexual and the following characteristics: they felt that HIV infection rate of MSM around them was high, and more agreed that “HIV is a threat to me/my family,” had a high frequency of seeking sexual partners through the Internet and alcohol drinking, had a history of STD, and felt being discriminated against by doctors and others. There is heterogeneity in the development trends of anxiety and depression. We need to improve intervention in the mental health of MSM, especially those with high anxiety and depression development trends.

Clinical Trial Registration

[http://www.chictr.org.cn/showproj.aspx?proj=5716], identifier [ChiCTR-TRC-13003849].
Can Sugarcane Yield and Health Be Altered with Fully Mechanized Management?
Authors: Xiao Jian , Liang Tian , Yang Shangdong , Tan Hongwei
Published: January 2023

At present, fully mechanized cultivation (FMC) has begun to be utilized in commercial sugarcane production in China. To provide new insights into whether cane yield and health are altered by fully mechanized cultivations, the cane yield and endophytic microbial community structure in stems of sugarcane that underwent fully mechanized cultivation (FMC) and conventional artificial cultivation (CAC) were compared. The results showed that the diversity and richness of endophytic microorganisms, except for the bacterial richness in the stems of sugarcane, could be significantly increased by using FMC. Meanwhile, in comparison with CAC, the relative abundance of Proteobacteria and Ascomycota increased under FMC. Moreover, some dominant endophytic bacterial genera, such as Acidovorax, Microbacterium, and Paenibacillus, and some dominant endophytic fungal genera, such as Scleroramularia, Tetraplosphaeria, and Dinemasporium, were found to be significantly enriched in cane stems under FMC treatments. Additionally, the endophytic microbial functions in sugarcane stems were not significantly altered by FMC treatments. Our results suggest that cane growth, yield, and health are not significantly altered by FMC. The results also indicate that fully mechanized management can be developed as a sustainable method in sugarcane production.