bluuebunny/crossref_metadata_2025 huggingface dataset:
Research of the LED emission mode impact on the productivity and physicochemical marks of Japanese cabbage in the tiered hydroponic module conditions
Authors: Knyazeva Inna , Vershinina Oksana , Grishin Andrei
Published: June 2024

Growth rates, plant biomass and the concentration of beneficial compounds largely depend on the quality and intensity of illumination. Plants of the ‘Mizuna Red’ variety were grown using a low-volume technology in a regulated agroecosystem of a tiered hydroponic module produced by VIM (Russia). The plants were illuminated by specially designed LED lamps manufactured by VIM (Russia) with a dy-namically controlled spectral composition in 4 channels. For experimental researches, the design of the lighting system included several modes of emission: continuous, pulsed and scanning with a radiation intensity of 15000 lux and a total PAR of 321 µmol m-2s-1: blue – 97 µmol m-2s-a; green – 84 µmol m-2s-1; red – 122 µmol m-2s-1; far red – 18 µmol m-2s-1 (Proportions B: G: R ~ 30:26:44). The aim of the study was to assess the effect of different modes of emission on productivity, physi-cochemical indicators and to develop technological methods for obtaining highquality commercial products of Japanese cabbage variety 'Mizuna Red' grown in a longline hydroponic module. The use of a pulsed emission mode made it possible to increase the photosynthetic activity of ‘Mizuna Red’ plants, which eventually influenced the growth of the aboveground mass and its quality indicators with a strong correlation between these indicators. The concentration of photosynthetic pigments was dependent on the emission mode.
Training of a Convolutional Neural Network for the Classification of Coffee Fruit State
Authors: Alvaro Fabricio Varela Marín , Aygumov Timur Gadzhievich , Matygov Movsar Musaevich
Published: June 2024

Convolutional neural networks are recognized for their high artificial intelligence capacity, mostly for their identification of objects either in images or videos. Honduras has a strong dependence on coffee cultivation, as it plays a crucial role in both its culture and economy. However, Honduras has endured difficulties linked to aspects such as quality, contamination, processing problems, illnesses, or lack of training. The root of problems often arises during the coffee harvest, and since Honduras is a less developed country, the use of advanced technology is not common. The recurring research aims to train a cnn model by using images and object detection to classify the quality of the coffee fruit. The training of a convolutional neural network was developed by using Robo Flow for the classification of the state of the coffee fruit. Firstly, the coffee farm was visited to see firsthand the fruit in its various stages. Due to the seasonal cuts established on the farms, a second visit was made to collect the coffee fruit. After that, the fruit was taken into a controlled environment to start the photo session. The session concluded with a total of 1702 photographs. The fruits within the images were individually annotated by classifying them into two categories: good and bad. A model result gave a precision, recall and mpa of 97.7%, 94.1% and 98.3%, respectively. The configurations were the use of 600 images, using a ratio of 80:10:10 for the division of training, validation, and testing tasks. Only one adjustment was made in the preprocessing, which consisted of changing the size of the images from 3000x4000 to 640x640. This is considered the best result after carrying out more training to test the alteration of the results of other models by making changes such as the number of images and the types of augmentations. Therefore, the results conclude the fulfillment of the objective.
Biomechanical Effects of Stem Extension of Tibial Components for Medial Tibial Bone Defects in Total Knee Arthroplasty: A Finite Element Study
Authors: Park Kwan Kyu , Kang Kyoung-Tak , Kwon Hyuck Min , Hong Hyoung-Taek , Kim Inuk , Cho Byung Woo , Koh Yong-Gon
Published: June 2024

The aim of this study was to investigate the biomechanical effects of stem extension with a medial tibial bone defect in primary total knee arthroplasty (TKA) on load distribution and stress in the proximal tibia using finite element (FE) analysis.

FE simulations were performed on the tibia bone to evaluate the stress and strain on the tibia bone and bone cement. This was done to investigate the stress shielding effect, stability of the tibia plate, and the biomechanical effects in TKA models with various medial defects and different stem length models.

The results demonstrated that in the bone defect model, the longer the stem, the lower the average von Mises stress on the cortical and trabecular bones. In particular, as the bone defect increased, the average von Mises stress on cortical and trabecular bones increased. The average increase in stress according to the size of the bone defect was smaller in the long stem than in the short stem. The maximal principal strain on the trabecular bone occurred mainly at the contact point on the distal end of the stem of the tibial implant. When a short stem was applied, the maximal principal strain on the trabecular bone was approximately 8% and 20% smaller than when a long stem was applied or when no stem was applied, respectively.

The findings suggest that a short stem extension of the tibial component could help achieve excellent biomechanical results when performing TKA with a medial tibial bone defect.
Liquid-assisted grinding (LAG) approach, metal-free synthesis of 2,3-dihydro-1,5-benzothiazepines and their electrochemical properties
Authors: Singh Manjit , Maurya Kuldeep Kumar , Malviya Manisha
Published: June 2024

In this work, we have disclosed a facile metal-free synthesis of pharmaceutically fascinating and biologically important benzothiazepines in the presence of a few drops of ethanol. The present methodology converts chalcones and ortho-amino thiophenol into 2,3-dihydro-1,5-benzothiazepines under ambient conditions using liquid-assisted grinding, resulting in rapid synthesis in a very short period. This process is eco-friendly, operationally simple and gives good to excellent yields of products with easy isolation. This approach offers a facile synthesis of 2,3-dihydro-1,5-benzothiazepines from three readily available starting materials. An electrochemical study of the benzothiazepine derivatives was undertaken using cyclic voltammetry and electrochemical impedance spectroscopy techniques.
Understanding the relationship between narrative identity, transdiagnostic factors and psychological functioning in a
young adult community sample
Authors: Corbett Monique , Reid Vincent , Bird Amy
Published: June 2024

Determining how narrative identity relates to psychological functioning is important for understanding how
psychopathology develops and is maintained. The present study investigated whether transdiagnostic factors mediate and/or moderate
associations of narrative identity with psychological functioning. We analysed data from n = 245 University
students who completed an online survey measuring turning point narratives, transdiagnostic factors and psychological functioning.
Results indicated that rumination and emotion dysregulation, but not overgeneral memory, mediated the relationship between lower
causal coherence and psychological functioning. Contrary to predictions, neither attachment security nor memory tone moderated any
relationships between narrative identity and psychological functioning. These pathways may be particularly important for
understanding the development and maintenance of psychopathology.
Baan Khoa Lak: A Case Study on Sustainable Rural Development through Community-Based Tourism and Natural Resource Conservation
Authors: Krittayaruangroj Krittawit , Suriyankietkaew Suparak
Published: June 2024

Baan Khoa Lak, a small village in Trang Province, Thailand, showcases rural sustainability. With around 183 families, it is a model for successful sustainable development. A standout initiative is the community-based tourism program, promoting sustainability while generating local income. Eco-tourism offerings like water rafting, Thai cooking classes, and homestays empower residents by involving them in decisions and nurturing a sense of ownership and belonging. A community forest diversifies tourism, safeguarding trees from harm, conserving resources, and upholding heritage. Baan Khoa Lak’s achievements earned it honors, including Trang Province’s cleanest village, Southern Region’s happiness village winner, and Thailand’s 2020 Best Responsible Tourism third prize. Community-based tourism uplifts living standards, spurs social connections, and fosters pride. Baan Khoa Lak epitomizes rural development’s potential with limited means. Through environmental and cultural preservation, it attracts nature-loving tourists, enriching both economy and residents’ lives.

Information

© The Authors 2024
Dendritic tip selection during solidification of alloys: Insights from phase-field simulations
Authors: Zhang 张 Qingjie 清杰 , Xing 邢 Hui 辉 , Wang 王 Lingjie 灵杰 , Zhai 翟 Wei 薇
Published: June 2024

The effect of undercooling ΔT and the interface energy anisotropy parameter ε
4 on the shape of the equiaxed dendritic tip has been investigated by using a quantitative phase-field model for solidification of binary alloys. It was found that the tip radius ρ increases and the tip shape amplitude coefficient A
4 decreases with the increase of the fitting range for all cases. The dendrite tip shape selection parameter σ* decreases and then stabilizes with the increase of the fitting range, and σ* increases with the increase of ε
4. The relationship between σ* and ε
4 follows a power-law function

σ
∗

∝

ε
4
α

, and α is independent of ΔT but dependent on the fitting range. Numerical results demonstrate that the predicted σ* is consistent with the curve of microscopic solvability theory (MST) for ε
4 < 0.02, and σ* obtained from our phase-field simulations is sensitive to the undercooling when ε
4 is fixed.
Orbital angular momentum conversion of acoustic vortex beams via planar lattice coupling
Authors: Han 韩 Qingbang 庆邦 , Liu 刘 Zhipeng 志鹏 , Yin 殷 Cheng 澄 , Wu 吴 Simeng 思梦 , Luo 罗 Yinlong 寅龙 , Yang 杨 Zixin 子鑫 , Pang 庞 Xiuyang 修洋 , Wang 王 Yiqiu 溢秋 , Kan 阚 Xuefen 雪芬 , Zhang 张 Yuqiu 雨秋 , Yu 俞 Qiang 强 , Wu 吴 Jian 坚
Published: June 2024

Orbital angular momentum (OAM) conversion is critical in understanding interactions between a structural sound field and a planar lattice. Herein, we explore the evolution of a monochromatic acoustic vortex beam (AVB) that is scattered by a phononic crystal (PnC) or a correlated random lattice. The phenomenon is ascribed to the enhanced orbit–orbit angular momentum coupling induced by the band structure. By modifying the coupling condition, accurate and continuous micro-manipulation of AVBs can be achieved, including the transverse/lateral gravity shift, the dynamics of the phase singularities, and the spatial distribution of acoustic pressure, etc. This research provides insight to the inhomogeneous coupling of AVBs with both propagating Bloch waves and localized Anderson modes, and may facilitate development of novel OAM-based acoustic devices for active sound field manipulation.
Three data-selection approaches for beam position monitor offline calibration
Authors: Kim Geunwoo , Kim Eun-San , Kwon Jang Won , Jang Siwon
Published: June 2024

In this study, we describe the fabrication of an
electrostatic beam position monitor (BPM) for beam diagnostic
systems. Calibration of the BPM pickup requires both sensitivity and
curve fitting owing to manufacturing tolerances following
fabrication. To calibrate the BPM, we developed a BPM calibration
system that comprises a wire-based test bench and readout
electronics. We considered three difference-over-sum (DOS) data
selection methods: DOSdiag, DOSaxis, and
DOSall, to understand their impact on calibration
accuracy. Each method selected specific DOS data, and we compared
their characteristic curves. Consequently, we analyzed the
calibration results by performing linear, 3rd-order, 5th-order, and
two-dimensional (2D) 3rd-order polynomial fitting. Each of the three
DOS data selection methods was examined to illustrate their
advantages and limitations in terms of calibration accuracy and
required amount of data. The results obtained demonstrated that
choosing the appropriate DOS data-selection approach could be
employed for the BPM offline calibrations, achieving minimized
calibration errors and optimized response characteristics with
polynomial curve fitting.
Assessment of NIR-triggered PEG-coated NaGdF4:Tm3+/Yb3+ bio-compatible upconversion nanoparticles for contrast enhancement in OCT imaging and optical thermometry
Authors: Shwetabh Kumar , Banerjee Abhishek , Poddar Raju , Kumar Kaushal
Published: June 2024

In this investigation, we embarked on the synthesis of polyethylene glycol coated NaGdF4:Tm3+/Yb3+ upconversion nanoparticles (UCNPs), aiming to assess their utility in enhancing image contrast within the context of swept source optical coherence tomography (OCT) and photo-thermal OCT imaging. Our research unveiled the remarkable UC emissions stemming from the transitions of Tm3+ ions, specifically the 1G4 → 3H6 transitions, yielding vibrant blue emissions at 472 nm. We delved further into the UC mechanism, meticulously scrutinizing decay times and the nanoparticles′ capacity to convert radiation into heat. Notably, these nanoparticles exhibited an impressive photo-thermal conversion efficiency of 37.5%. Furthermore, our investigations into their bio-compatibility revealed a promising outcome, with more than 90% cell survival after 24 h of incubation with HeLa cells treated with UCNPs. The nanoparticles demonstrated a notable thermal sensitivity of 4.7 × 10−3 K−1 at 300 K, signifying their potential for precise temperature monitoring at the cellular level.
Model-based agricultural landscape assessments: a review
Authors: Heidenreich Anja , Muller Adrian , Oggiano Philipp , Pfeifer Catherine , Moakes Simon , Six Johan , Stolze Mathias
Published: June 2024

Agricultural landscapes are multifunctional and closely connected to the much wider food system. In our review, we explore three specific aspects of modelling approaches for agricultural landscape assessments: (a) how multifunctionality is commonly analysed to support decision-making for sustainable agricultural land management; (b) how the modelling approaches relate to the wider food systems; and (c) how gaps in the existing modelling approaches might be addressed. For this, we identified key elements of agricultural landscape assessments, including ecosystem services (ESS) provided, driving factors, and linkages between crop and livestock production, and to the wider food system. We reviewed 238 publications with respect to these elements. While biodiversity and the ESS ‘water conditions’ and ‘atmospheric composition/conditions’ are widely covered, they are rarely used in combination. Other ESS, such as ‘pest and disease control’, are largely missing. Our results further indicate strong differences between individual approaches regarding model parameterisation and consideration of abiotic, biotic, and management driving factors. Our analysis also shows that the interconnectedness of crop and livestock production is rarely considered and that return flows from the food system are not considered. Furthermore, impacts from the production of external inputs and off-site effects are not considered. Consequently, existing models might overlook trade-offs and synergies between landscape functions. Failure to consider variations in relevant driving factors and food system linkages likely results in studying incorrect levers for change and failing to show decision-makers the full scope of available action. We thus suggest adopting more encompassing modelling approaches to ensure coverage of the full scope of potential actions, whilst hedging against overly costly data requirements by, e.g. employing well-designed sensitivity analyses. In this way, the most relevant components and thus the most important leverage points for interventions can be identified.
A note on orientation-reversing distance one surgeries on non-null-homologous knots
Authors: Ito Tetsuya
Published: June 2024

We show that there are no distance one surgeries on non-null-homologous knots in

M
M

that yield

−
M

-M

(

M
M

with opposite orientation) if

M
M

is a 3-manifold obtained by a Dehn surgery on a knot

K
K

in

S

3

S^{3}

, such that the order of its first homology is divisible by

9
9

but is not divisible by

27
27

.

As an application, we show several knots, including the

(
2
,
9
)

(2,9)

torus knot, do not have chirally cosmetic bandings. This simplifies the proof of a result first proven by Yang that the

(
2
,
k
)

(2,k)

torus knot

(
k
>
1
)

(k>1)

has a chirally cosmetic banding if and only if

k
=
5

k=5

.
A closure result for globally hyperbolic spacetimes
Authors: Catino Giovanni , Roncoroni Alberto
Published: June 2024

In this paper we prove a closure result for globally hyperbolic spacetimes satisfying, at a certain time, natural assumptions on the deceleration, the pressure and the Hubble constant. The main tool that we use is a general Bonnet-Myers type result.
Human trophoblast invasion and migration are mediated by the YAP1-CCN1 pathway: defective signaling in trophoblasts during early-onset severe preeclampsia
Authors: Wu Liang , Wang Shengfu , Li Hongyue , Lu Haotian , Zheng Yuanke , Feng Tianfei , Sun Yingpu
Published: June 2024

The transcription coactivator YAP1 mediates the major effects of the Hippo signaling pathway. The CCN family is a small group of glycoproteins known to be downstream effectors of YAP1 in diverse tissues. However, whether CCN family members mediate the effects of YAP1 in human trophoblasts is unknown. In this study, placental expression of both YAP1 and CCN1 was found to be impaired in pregnancies complicated by early-onset severe preeclampsia. CCN1 was expressed not only in cytotrophoblasts, trophoblast columns, and mesenchymal cells, similar to active YAP1, but also in syncytiotrophoblasts of normal first-trimester placental villi; moreover, decidual staining of active YAP1 and CCN1 was found in both interstitial and endovascular extravillous trophoblasts. In cultured immortalized human trophoblastic HTR-8/SVneo cells, knockdown of YAP1 decreased CCN1 mRNA and protein expression and led to impaired cell invasion and migration. Also, CCN1 knockdown negatively affected HTR-8/SVneo cell invasion and migration but not viability. YAP1 knockdown was further found to impair HTR-8/SVneo cell viability via G0/G1 cell cycle arrest and apoptosis, while CCN1 knockdown had minimal effect on cell cycle arrest and no effect on apoptosis. Accordingly, treatment with recombinant CCN1 partially reversed the YAP1 knockdown-induced impairment in trophoblast invasion and migration but not in viability. Thus, CCN1 mediates the effects of YAP1 on human trophoblast invasion and migration but not apoptosis, and decreased placental expression of YAP1 and CCN1 in pregnancies complicated by early-onset severe preeclampsia might contribute to the pathogenesis of this disease.
SCUBE2 regulates adherens junction dynamics and vascular barrier function during inflammation
Authors: Lin Yuh-Charn , Chang Ya-Jen , Gau Shiang-Shin , Lo Chun-Min , Yang Ruey-Bing
Published: June 2024

Aims

SCUBE2 (signal peptide-CUB-epidermal growth factor-like domain-containing protein 2) is a secreted or membrane-bound protein originally identified from endothelial cells (ECs). Our previous work showed that SCUBE2 forms a complex with E-cadherin and stabilizes epithelial adherens junctions (AJs) to promote epithelial phenotypes. However, it remains unclear whether SCUBE2 also interacts with vascular endothelial (VE)-cadherin and modulates EC barrier function. In this study, we investigated whether and how SCUBE2 in ECs regulates vascular barrier maintenance.

Methods and results

We showed that SCUBE2 colocalized and interacted with VE-cadherin and VE-protein tyrosine phosphatase (VE-PTP) within EC AJs. Furthermore, SCUBE2 knockdown disrupted EC AJs and increased EC permeability. Expression of EC SCUBE2 was suppressed at both mRNA and protein levels via the nuclear factor-κB signalling pathway in response to pro-inflammatory cytokines or permeability-inducing agents. In line with these findings, EC-specific deletion of Scube2 (EC-KO) in mice impaired baseline barrier function and worsened vascular leakiness of peripheral capillaries after local injection of histamine or vascular endothelial growth factor. EC-KO mice were also sensitive to pulmonary vascular hyperpermeability and leucocyte infiltration in response to acute endotoxin- or influenza virus-induced systemic inflammation. Meanwhile, EC-specific SCUBE2-overexpressing mice were protected from these effects. Molecular studies suggested that SCUBE2 acts as a scaffold molecule enabling VE-PTP to dephosphorylate VE-cadherin, which prevents VE-cadherin internalization and stabilizes EC AJs. As such, loss of SCUBE2 resulted in hyperphosphorylation of VE-cadherin at tyrosine 685, which led to its endocytosis, thus destabilizing EC AJs and reducing barrier function. All of these effects were exacerbated by inflammatory insults.

Conclusion

We found that SCUBE2 contributes to vascular integrity by recruiting VE-PTP to dephosphorylate VE-cadherin and stabilize AJs, thereby promoting EC barrier function. Moreover, our data suggest that genetic overexpression or pharmacological up-regulation of SCUBE2 may help to prevent vascular leakage and oedema in inflammatory diseases.
Reef fish assemblages differ both compositionally and functionally on artificial and natural reefs in the eastern Gulf of Mexico
Authors: Schram Michael J , Emory Meaghan E , Kilborn Joshua P , Peake Jonathan A , Wall Kara R , Williams Ian , Stallings Christopher D
Published: June 2024

Human-made structures, particularly artificial reefs, have played an increasingly important role in restoration, conservation, and management efforts aimed at mitigating the detrimental effects of anthropogenic activities on habitats and their associated communities. However, the extent to which artificial reefs support marine assemblages resembling those of natural habitat remains unclear. In this study, we used a 10-year dataset of reef fish visual surveys performed on paired reefs located in the eastern Gulf of Mexico, complemented by functional trait data, to examine whether artificial and natural reefs support fish assemblages with similar taxonomic and functional diversities. Our results revealed compositionally and functionally distinct assemblages between reef types, a pattern that was consistent across depths. The inclusion of functional diversity allowed us to explicitly identify generalized patterns in the composition of these assemblages that may have only been inferred from a strictly taxonomic-based approach. Specifically, large-bodied, midwater predators were relatively more abundant on artificial reefs, whereas smaller-bodied, benthic-associated invertivores or mesopredators were more frequently observed on natural reefs. The artificial reefs in this study were previously characterized as having greater vertical relief than their natural counterparts, a feature that likely contributed to the greater abundances of midwater predators on those reefs. Similarly, natural reefs typically offered a greater abundance of medium-to-small refugia necessary for smaller-bodied taxa to forage and avoid predation. Previous work also demonstrated that these artificial reefs were visited 2–10 times more frequently by boaters than natural reefs, presumably for recreational fishing purposes. These findings led us to infer that local-scale environmental filtering and variable predator–prey dynamics resulting from differences in the physical characteristics of these reefs coupled with asymmetric fishing activity influenced the observed differences among reef fish assemblages. Additionally, our work underscored the utility of multiple diversity measures in comparative assemblage studies, provided insight into reef-fish assemblage dynamics, and helped to emphasize a pressing need to better understand the role of human-made structures in marine ecosystems.
Research on carbon-neutral calculation model of urban parks based on life-cycle assessment: a case study from Beijing, China
Authors: Hu Congan , Liu Guanyan , Su Peng , Lan Fujie , Zhang Tao , Xie Wei , Liang Yi , Wang Xin , Jieensi Ahebai , Liu Pengfei
Published: June 2024

Urban parks are an essential component of the urban carbon sink system. Calculating a city’s overall carbon balance is significant by predicting when urban parks will achieve carbon-neutral. This research is based on the theory of life-cycle assessment and uses carbon emission factors to calculate the project’s carbon emissions. Using the established model, we calculated the urban park with a total area of 6.5 hectares in a certain city in Fangshan District, Beijing, China. The results indicate that the carbon emission intensity of the city park during the construction materials production phase is $5.97$${\mathrm{tCO}}_2\mathrm{e}/\mathrm{ha}$; during the construction phase, it is 18.947${\mathrm{tCO}}_2\mathrm{e}/\mathrm{ha}$; and during the operation phase, it is $5.97{\mathrm{tCO}}_2\mathrm{e}/\mathrm{ha}/\mathrm{Year}$. The total carbon reduction of the photovoltaic system in the first 25 years is $260.007{\mathrm{tCO}}_2/\mathrm{ha}$. Carbon sinks have a relatively minor impact on achieving carbon neutrality goals for urban parks. The case urban park will take 20–21 years to achieve the carbon-neutral target. We propose a future low-carbon strategy for urban parks through the study results. This will also contribute to developing uniform evaluation standards for carbon neutrality in urban parks.
(171) EFFECT OF FINASTERIDE TREATMENT AND ITS WITHDRAWAL IN THE RAT CORPUS CAVERNOSUM
Authors: Giatti Prof Silvia , Diviccaro Silvia , Cioffi Lucia , Mitro Prof Nico , Caruso Prof Donatella , Melcangi Prof Roberto C
Published: June 2024

Objectives

Finasteride is an inhibitor of the 5α-reductase used for treating androgenetic alopecia (AGA). However, despite its efficacy, it may cause sexual alterations like erectile dysfunction (ED) that can persist after drug discontinuation, with the occurrence of post-finasteride syndrome (PFS). We previously described ED and altered pudendal nerve transmission in PFS patients treated for AGA, but the molecular mechanisms have not yet been explored. Therefore, we analyzed the effect of finasteride treatment and its withdrawal in an animal model.

Methods

Adult male rats were treated with finasteride (1 mg/day/rat) or vehicle (sesame oil) for 20 days and sacrificed 24h or 30 days after the last injection. The levels of androgens and catecholamines were assessed by mass spectrometry in the corpus cavernosum (CC) and plasma, respectively. In addition, targeted metabolomics coupled with a nitrate/nitrite colorimetric assay, and western blotting was performed in CC.

Results

Molecular alteration suggestive of ED were reported after treatment. Indeed, we observed the following: 1) a significant increase in the plasma levels of norepinephrine, and a decrease in those of epinephrine; 2) decreased protein levels of 5α-reductase type II in the CC, in line with increased testosterone and decreased dihydrotestosterone levels; 3) a reduction in the activity of nitric oxide synthase (NOS) and ornithine transcarbamylase (OTC); 4) a correlation between testosterone levels and NOS activity, and between dihydrotestosterone levels and OCT activity. No alterations in these parameters were observed at withdrawal.

Conclusions

These observations suggest that finasteride treatment, but not its withdrawal, induces molecular alterations in male rats that may be linked to erectile dysfunction. Considering that the nervous system is affected in PFS patients and animal models, and that male sexual behavior is under the control of complex neural circuits, future experiments will be dedicated to exploring whether finasteride withdrawal affects these circuits.

Conflicts of Interest

Authors declare no conflicts of interest.
(175) CHANGES IN ORGASMIC FUNCTION FOLLOWING RADICAL PROSTATECTOMY: A RETROSPECTIVE COHORT STUDY
Authors: Jørgensen Anna C , Jensen Christian FS , Rikanovic Mira , Fode Mikkel
Published: June 2024

Objectives

To explore rates of anorgasmia and subjective changes in orgasm following robot-assisted radical prostatectomy (RARP) and to identify possible predicting factors of orgasmic alterations.

Methods

We performed a retrospective chart review of men who participated in a post-RARP sexual rehabilitation consultation at Herlev and Gentofte Hospital between 2017 and 2021. All consultations were performed by the same specialized nurse. The men were asked if they could achieve an orgasm. If confirmed, they were asked which one of 6 descriptions best described their orgasm compared to before RARP. Associations between orgasmic alterations and nerve-sparing surgery were analyzed by Pearsons χ2-tests. Associations between orgasmic alterations and time since surgery as well as age were analyzed by logistic regression.

Results

237 charts contained information about orgasmic function. The mean age at the time of consultation was 65 years (range 39 to 79). Mean time since surgery was 15 months. 42 and 145 men had undergone bilateral and unilateral nerve-sparing RARP respectively, while 50 had not had nerve-sparing. 40% (CI 34%-46%) experienced unchanged orgasms, while 11% (CI 8%-16%) suffered from anorgasmia. Another 11% (CI 8%-16%) had difficulty achieving an orgasm, 11% (CI 8%-16%) experienced decreased intensity, and 22% (CI 17%-27%) had both difficulty achieving an orgasm and experienced it with decreased intensity. 3% (CI 2%-7%) experienced orgasms with increased intensity. The last 1% (CI 0%-4%) had painful orgasms. The only significant association between orgasmic alterations and any predicting factor was between higher age and anorgasmia (p = 0.03). However, the risk of anorgasmia increased only 0.074% per year after the age of 65.

Conclusions

Changes in orgasmic function is common after RARP. There does not seem to be clear predictors of these issues. This underlines the importance of addressing orgasmic function in conjunction with RARP.

Conflicts of Interest

No conflicts of Interest.
(239) PRP AS A THERAPEUTIC OPTION IN PEYRONIE’S DISEASE
Authors: Ditonno Prof Pasquale , Mongelli Dr Luca , Guglielmi Dr Giuseppe , Dachille Dr Giuseppe
Published: June 2024

Objectives

This study is a prospective and single-center experimental investigation involving 57 individuals diagnosed with Peyronie's disease. The participants in this study received treatment through injections of Platelet-Rich Plasma directly into the plaques. The aim is to evaluate effectiveness of PRP in improving penile curvature and plaque size, which are key features of Peyronie's disease.

Methods

The inclusion criteria encompassed patients with PD who showed no response to oral medication, exhibited a curvature angle ranging between 30° and 90°, platelet counts within the normal range (> 150,000/μL). Excluded from the study were patients with PD who had previously undergone surgery, collagenase or hyaluronic acid therapy, shock wave treatment. Patients underwent three comprehensive evaluations: baseline assessment at recruitment (T0), a second evaluation 2 weeks later (T1), and a third evaluation 4 weeks after the third and final PRP injection (T2). T1 and T2 included the assessment of PD-related information: curvature angle (measured using a goniometer and documented with photography), curvature direction (dorsal, ventral, right lateral, left lateral), ultrasound measurements of plaque (in millimeters), the presence of calcifications, possible circulatory abnormalities assessed via dynamic penile eco-color Doppler with pharmacological stimulation, measurements of erect penis length and circumference, and reports of any side effects or unwanted reactions such as local reactions, bruising, pain at the injection site, fever, signs of infection. Self-administered evaluation questionnaires were used: IIEF-5; PDQ, designed specifically for Peyronie's disease.

Results

The average penile curvature angle decreased significantly. The IIEF score demonstrated significant improvement post-treatment. Importantly, no side effects were observed during the study period.

Conclusions

PRP plays a valuable role in slowing down the advancement of Peyronie's diseaset to more severe stages. Our research underscores the effectiveness of this treatment and its notable lack of substantial adverse effects. Consequently, PRP emerges as a cost-effective, non-invasive therapeutic choice for Peyronie's disease patients.

Conflicts of Interest

None.
(1024) HYALURONIC ACID TREATMENT AND REDUCTION OF SURGICAL INTERVENTION INCIDENCE IN PEYRONIE'S DISEASE: A COMPARISON BETWEEN TWO GROUPS
Authors: Marino Dr Claudio , Giordano Dr Alessandro , D'Alterio Dr Carlo , Zimarra Dr Assunta , Magliocchetti Dr Marco , Sansone Dr Catello , Capece Dr Marco
Published: June 2024

Objectives

Hyaluronic Acid (HA) plays a crucial role in immune modulation and anti-inflammatory effects. HAyaluronic acid is currently under investigation for various conditions, including premature ejaculation, penile enlargement, and Peyronie's disease. Notably, we focus on evaluating the reduction in the incidence of surgical interventions in patients treated with HA. This study aims to compare the reduction in surgical intervention incidence between two groups, specifically evaluating the percentage of patients with residual penile curvature <40° after treatment at 6 months adn one year follow-up.

Methods

From 2020 to 2022, 70 patients with chronic-phase Peyronie's disease were treated with intraplaque HA injections every other week for six weeks plus penile modeling after each treatment. Furthermore, each patient performed a daily use of vacuum erection device combined with home (self) modeling and stretching in the days following the injections. A comparative retrospective analysis included another 70 patients from 2017 to 2018 who met similar inclusion criteria but had not received HA treatment. The control group included 70 patients treated with PDE5 inhibitors and anti-inflammatories.

Results

In the hyaluronic acid (HA)-treated group (Group 1), 75% of subjects demonstrated a significant improvement in residual curvature, measuring less than 40° at both the 6-month and one-year follow-up assessments. This improvement in curvature was found to be statistically significant and resulted in a substantial reduction in the necessity for surgical intervention. In contrast, only 20% of Group 2 (control) patients avoided surgery after one year, with minimal curvature changes (±10°, p < 0.0001), highlighting the statistical significance of the outcome differences between the two groups.

Conclusions

The comparative analysis underscores Hyaluronic Acid's potential not only in alleviating residual curvature but also in significantly reducing the incidence of surgical interventions. This study advocates for the consideration of HA treatment as a viable option in managing Peyronie's disease, providing valuable insights for future research and clinical applications.

Conflicts of Interest

I declare that there is no conflict of interest regarding the research presented in this abstract. I, the undersigned author, affirm that no financial or personal relationships with other individuals or organizations have influenced the content or outcomes of this study.
(132) THE STUDY PROTOCOL OF AN ONGOING RANDOMIZED PILOT STUDY FUNDED BY THE ESSM: FEASIBILITY AND EFFICACY OF COGNITIVE BEHAVIORAL SEX THERAPY (CBT-SEX) AS AN ADJUNCT SHORT-TERM INTERVENTION FOR PEYRONIE’S DISEASE (PD)
Authors: Soave Priv-Doz Armin , Buchholz Anika , Hinz Johannes , Fisch Prof Margit , Schäfer Lukas , Weber Philipp , Nieder Priv-Doz Timo
Published: June 2024

Objectives

PD is a connective tissue disorder of the penis resulting in penile deformity and curvature. Consequently, PD patients complain severe impairment of sexual activity and satisfaction, and suffer from detrimental psychological sequelae. Currently, the feasibility and efficacy of CBT-sex as an adjunct short-term intervention for patients undergoing conservative and surgical therapy has not been evaluated. Therefore, there is an urgent need for a pilot study on the feasibility and efficacy of CBT-sex in PD.

Methods

Randomized, open, mono-centric feasibility study. PD patients, who are scheduled for either conservative or surgical treatment, are enrolled. Patients are randomized to receive either CBT-sex (CBT+) or no CBT-sex (CBT-), stratified by treatment (conservative or surgical therapy) using block randomization. The CBT+ group receives six CBT-sex sessions (one session per week for 6 weeks) by one certified psychotherapist.

Results: Co-primary endpoints

Secondary endpoints

Conclusions

We present the study protocol of this ongoing study, which will generate first data on CBT-sex to reduce PD-induced stress, symptoms and burdens and to improve the sexual satisfaction and erectile function.

Conflicts of Interest

No conflicts of interest.
(331) DOES VARICOCELECTOMY INCREASE THE SUCCESS RATE OF MICRODıSSECTION TESTICULAR SPERM EXTRACTION OPERATION IN NON-OBSTRUCTIVE AZOOSPERMIC MEN?
Authors: Sahin Dr Kadir C , Gultekin Dr Mehmet H , Ozkara Prof Hamdi , Akkus Prof Emre
Published: June 2024

Objectives

Varicocele is detected on physical examination in some non-obstructive azoospermic (NOA) men. However, the current literature is not clear about the effect of varicocelectomy on sperm retrieval rates (SRR) in microdissection testicular sperm extraction (mTESE) in NOA men. Our aim was to investigate the effect of varicocele on SRR rates in patients whom we performed mTESE operations due to NOA in our clinic.

Methods

The data of patients who underwent mTESE operation between 2000 and 2019 in our clinic were retrospectively analyzed. Along with clinical and demographic data, patients’ SRR were compiled. Patients with Klinefelter's disease, solitary testis, or missing data were excluded from the study. The results were comparatively analyzed with SPSS v.25 (Armonk, NY: IBM Corp.).

Results

In our clinic, 651 patients who underwent mTESE between the specified dates and who satisfied the exclusion and inclusion criteria were included in the study. 224 patients were diagnosed with varicocele and 90 (40.17%) of these patients underwent varicocelectomy operation. Of the varicocelectomy operations, 78 (86.67%) were unilateral and 12 (13.33%) were bilateral. The SRR of patients who underwent mTESE based on varicocele absence/presence and varicocelectomy history were given in table. Analysis of the data from these three groups revealed that there was no statistically significant difference in SRR between varicocele diagnosis and varicocelectomy (p=0.99).

Conclusions

According to the results of our study, neither the presence of varicocele nor the history of varicocelectomy had a positive or negative impact on the SRR of the patients. Therefore, care should be taken in the decision-making process, particularly in patients who have varicocele and are candidates for mTESE.

Conflicts of Interest

All authors declare that they have no conflicts of interest.
(320) TREATMENTS FOR PUBERTAL INDUCTION IN BOYS: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Yannas Dr Dimitri , Vena Dr Walter , Betella Dr Nazarena , Zago Dr Elena , Maseroli Dr Elisa , Di Stasi Dr Vincenza , Cipriani Dr Sarah , Pizzocaro Dr Alessandro , Maggi Prof Mario , Vignozzi Prof Linda , Rastrelli Prof Giulia
Published: June 2024

Objectives

Delayed puberty (DP) is a common clinical condition in boys. When DP is due to underlying pathologies or it causes profound social distress, pubertal induction is undertaken although a standard treatment protocol is not recommended. This is a systematic review and meta-analysis of literature aimed to summarize the therapeutic approaches used in published researches on male DP and to assess the possible differences in achieved outcomes.

Methods

An Medline search was conducted, including the following research key: (hypogonadotropic hypogonadism OR delayed puberty OR congenital delay of puberty) AND (therapy OR protocol OR treatment) AND (pubert*). The search was conducted until July 1st 2023.

Results

27 articles where obtained for the meta-analysis, including 47 studies. Of these, 33, 25, 20, and 13 reported data on testicular volume (TV), total testosterone (TT), height velocity, and final height after treatment for pubertal induction.

TV after treatment was 7.66 [6.68-8.65] mL, similar in studies using treatments mimicking the hypothalamus-pituitary activity and those using androgens (Q=2.33, p=0.127). Worse results were obtained by regimens using only hCG as compared with GnRH, FSH+hCG with or without FSH priming (Q=21.55, p<0.0001). Among the androgen preparations, mesterolone was associated with the lowest TV, as compared with oxandrolone and testosterone enanthate (Q=16.62, p<0.0001).

Androgens were associated with the highest HV (Q=7.74, p=0.05 for the comparison with hypothalamus-pituitary treatments), with studies using testosterone enanthate reporting the highest HV (Q=4.28; p=0.038). However, no differences in final height were detected among the different treatment regimens.

Conclusions

Despite some differences are observed between treatments mimicking the activity of the hypothalamus-pituitary axis and androgens, the final testicular volume and final height are similar. This does not support the superiority of one specific approach. The use of hCG alone represents and exception because it is associated with significantly lower TV achieved at the end of the treatment.

Conflicts of Interest

The authors declare no conflict of interest.
(227) THE ROLE AND PLACE OF QUANTITIVE ANALYSIS FOR PSYCHO-DIAGNOSTIC DATA IN THE PROCESS OF DIAGNOSIS OF FEMALE SEXUAL DYSFUNCTION
Authors: Marshania Zurab , Chochnidze Nino , Injgia Marika
Published: June 2024

Objectives

Sexual activity is an important aspect of quality of life. It's a fact that mental disorders caused by sexual trauma in the past, bullying, strict upbringing, stigmatization, and low level of sexual education, play important roles in the development of female sexual dysfunctions. The aim of our study was to detect how effective is psycho-diagnostic examination for timely diagnosis and adequate treatment of women with sexual dysfunctions.

Methods

In our research that was conducted from October 2022 to July 2023, at Zurab Marshania's sexual medicine outpatient office, 75 women with different sexual dysfunctions. The study was quantitative. We used a correlational analysis to assess the association between dependent and independent variables. Independent variables with a p-value < 0.05 were taken as statistically significant with sexual dysfunction. To evaluate psychological characteristics, we took MMPI-1, SSTAI, and the Beck Depression Questionnaire.

Results

All our patients had different sexual difficulties. 55% among 75 had psychological problems that were identified before the sexual dysfunction was discovered. The largest correlation (53%) was found between anxiety disorders and Female Orgasmic Disorder (FOD), as well as between depression and Female Hypoactive Sexual Desire Disorder (FHSDD) which was 27%. It is also worth emphasizing the style of life caused by strict traditional family education, due to which some of the patients had an inadequate perception of sexual life (63%).

Conclusions

The prevalence of sexual dysfunction among women with mental disorders is quite high, therefore, along with the treatment of sexual dysfunctions, it is important to properly evaluate the psychological state that can be performed effectively by psycho-diagnostic examination. Without it, the treatment of sexual dysfunctions among women will not be managed adequately.

Conflicts of Interest

there are no conflicts of Interest.
(389) EXPLORING THE LINK BETWEEN CONFORMITY TO MASCULINE IDEOLOGIES AND USE OF COERCIVE AND COAXING TACTICS FOLLOWING SEXUAL REJECTION
Authors: Birke Jennifer , Hochleitner Prof Margarethe , Komlenac Priv-Doz Nikola
Published: June 2024

Objectives

To investigate the link between conformity to traditional masculine ideologies (TMIs) and the use of coercive (forceful and manipulative) and coaxing (benign or seductive) tactics following sexual rejection in cisgender men and women.

Methods

German-speaking participants (N = 771; 49.5% men and 50.5% women) were randomly assigned to read one of two hypothetical scenarios involving sexual rejection or engagement in sexual activities. Participants reported their reactions to sexual rejection. Additionally, conformity to TMIs were assessed. Hierarchical regression analysis was employed for data analysis.

Results

Participants with higher interest in partnered sexual activities, and higher enactment of power over women more often would use coercive tactics, as opposed to participants with lower conformity to those TMIs. Conversely, lower emotional restriction is associated with more frequent use of coercive tactics. Among men with higher conformity to social status, those who were rejected more often would use coaxing tactics, compared to those who were not rejected. Conversely, among participants with higher interest in partnered sexual activities, those who were not rejected more often would use coaxing tactics compared to those who were rejected.

Conclusions

Conformity to masculinity ideologies might be risk factors for women and men to use coercion tactics during partnered sexual activities. Therefore, sexual activities might be a means of achieving social status. Additionally, a desire to appear sexually experienced can contribute to the risk of resorting to coercion. Furthermore, coercive tactics may be used as an outlet for expressing frustration arising from perceived power imbalances within relationships. Interventions should employ gender-transformative strategies to separate sexual experiences from the pursuit of social status and provide individuals with alternative communication and conflict resolution techniques, emphasizing the importance of navigating power dynamics in a healthy and non-coercive manner. This transformative shift advances efforts to prevent and address sexual coercion by challenging deeply rooted stereotypes.

Conflicts of Interest

No conflicts of interest to declare.
Metabolic profiling of glioblastoma stem cells reveals pyruvate carboxylase as a critical survival factor and potential therapeutic target
Authors: Renoult Ophélie , Laurent--Blond Mélanie , Awada Hala , Oliver Lisa , Joalland Noémie , Croyal Mikaël , Paris François , Gratas Catherine , Pecqueur Claire
Published: June 2024

Background

Glioblastoma (GBM) is a highly aggressive tumor with unmet therapeutic needs, which can be explained by extensive intra-tumoral heterogeneity and plasticity. In this study, we aimed to investigate the specific metabolic features of Glioblastoma stem cells (GSC), a rare tumor subpopulation involved in tumor growth and therapy resistance.

Methods

We conducted comprehensive analyses of primary patient-derived GBM cultures and GSC-enriched cultures of human GBM cell lines using state-of-the-art molecular, metabolic, and phenotypic studies.

Results

We showed that GSC-enriched cultures display distinct glycolytic profiles compared with differentiated tumor cells. Further analysis revealed that GSC relies on pyruvate carboxylase (PC) activity for survival and self-renewal capacity. Interestingly, inhibition of PC led to GSC death, particularly when the glutamine pool was low, and increased differentiation. Finally, while GSC displayed resistance to the chemotherapy drug etoposide, genetic or pharmacological inhibition of PC restored etoposide sensitivity in GSC, both in vitro and in orthotopic murine models.

Conclusions

Our findings demonstrate the critical role of PC in GSC metabolism, survival, and escape to etoposide. They also highlight PC as a therapeutic target to overcome therapy resistance in GBM.