Proliferation of human bone marrow stem cells cultured on alfa-tricalcium phosphate cement and titanium discs
Authors: De Paris Marcel Fasolo , Baraldi Carlos Eduardo , Viezzer Christian , Machado Denise Cantarelli , Gerhardt de Oliveira Marília 
Published: November 2017

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Methods: discs of </span>α<span style="mso-ansi-language: EN-US;" lang="EN-US"> -TCP and commercially pure Ti weremanufactured and placed on polyethylene plates. Cells were cultured according to the following: group I – control (no discs); group II – </span>α<span style="mso-ansi-language: EN-US;" lang="EN-US">-TCP discs; group III – Ti discs. Propidium iodide staining was used to assess cell proliferation. Cells cultures were analyzed at 3,7, 14 and 21 days. Results: Proliferation occurred in both biomaterials. At 3 days, greater cell proliferation was seen in group II than group III (p=0.01). Differences were not significant on the other time periods. Morphologic analysis showed circular morphology in group I, compatible with osteogenic cell lineage. Conclusions: both biomaterials were osteoconductive but not osteoinductive. 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