bluuebunny/crossref_metadata_2025 huggingface dataset:
База, деревня, природа: трансформация представления местных жителей о поселке городского типа во второй половине XX — начале XXI в. / Base, Village, Nature: Transformation of the Local Image of an Urban-type Settlement in the Second Half of the 20th — Early 21st Centuries
Authors: Желамская Е.П.
Published: June 2024

Статья посвящена исследованию статуса поселка городского типа (ПГТ) второй половины XX — начала XXI в., а именно анализу представлений о сельском и городском, о границе сельского и городского в восприятии современных жителей южной части Архангельской области. При помощи концептов «первого», «второго» и «не-места» (по М. Оже), была предпринята попытка исследования с этнографической точки зрения советского проекта по преобразованию сельского поселения в город. На основе полевого материла и анализа газетных статей автор показывает, что официальный взгляд на ПГТ как на модерновый, городской населенный пункт не совпадает с мнением жителей, участвовавших в реализации этого проекта. Отношение к поселку, созданному преимущество переселенцами из сельской среды, на протяжении времени менялось от восприятия его как рабочего места в лесу к попытке создания жилого пространства на основе деревенского опыта. Далее оценка поселка вновь трансформировалась, он стал видеться как территория, не до конца преобразованная в городской район и, наконец, еще позднее как перевалочный пункт по дороге из деревни в настоящий город и наоборот.
The article is devoted to the study of the urban–type settlement status in the second half of the 20th — beginning of the 21st centuries, specifically, to the analysis of ideas about rural and urban and the boundaries between rural and urban as seen by modern residents of the southern part of the Arkhangelsk region. This paper studies from an ethnographic point of view the Soviet project of transformation of a rural settlement into an urban one using the concepts of "first", "second" places and "non-places" (following M. Augé). Based on field material and newspaper articles, the author shows that the official vision of the urban–type settlement as a modern urban settlement does not coincide with how the residents, who took part in the implementation of this project, see it. The first settlers came from rural areas and their vision of the urban-type settlement had been changing from a workplace in the forest to an attempt to organize a living space based on the village experience. Than it started to be perceived as a territory, which has not been fully urbanized, and, finally, as a transit point on the way from the village to real cities and vice versa.
Endothelial-derived microvesicles promote pro-migratory cross-talk with smooth muscle cells by a mechanism requiring tissue factor and PAR2 activation
Authors: Featherby Sophie J. , Ettelaie Camille
Published: June 2024

Introduction

Microvesicles (MV) released by endothelial cells (EC) following injury or inflammation contain tissue factor (TF) and mediate communication with the underlying smooth muscle cells (SMC). Ser253-phosphorylated TF co-localizes with filamin A at the leading edge of migrating SMC. In this study, the influence of endothelial-derived TF-MV, on human coronary artery SMC (HCASMC) migration was examined.

Methods and Results

MV derived from human coronary artery EC (HCAEC) expressing TFWt accelerated HCASMC migration, but was lower with cytoplasmic domain-deleted TF. Furthermore, incubation with TFAsp253-MV, or expression of TFAsp253 in HCASMC, reduced cell migration. Blocking TF-factor VIIa (TF-fVIIa) procoagulant/protease activity, or inhibiting PAR2 signaling on HCASMC, abolished the accelerated migration. Incubation with fVIIa alone increased HCASMC migration, but was significantly enhanced on supplementation with TF. Neither recombinant TF alone, factor Xa, nor PAR2-activating peptide (SLIGKV) influenced cell migration. In other experiments, HCASMC were transfected with peptides corresponding to the cytoplasmic domain of TF prior to stimulation with TF-fVIIa. Cell migration was suppressed only when the peptides were phosphorylated at position of Ser253. Expression of mutant forms of filamin A in HCASMC indicated that the enhancement of migration by TF but not by PDGF-BB, was dependent on the presence of repeat-24 within filamin A. Incubation of HCASMC with TFWt-MV significantly reduced the levels of Smoothelin-B protein, and upregulated FAK expression.

Discussion

In conclusion, Ser253-phosphorylated TF and fVIIa released as MV-cargo by EC, act in conjunction with PAR2 on SMC to promote migration and may be crucial for normal arterial homeostasis as well as, during development of vascular disease.
Assessment of accessibility in virtual laboratories: a systematic review
Authors: Deriba Fitsum G. , Saqr Mohammed , Tukiainen Markku
Published: June 2024

In an era of rapid evolution in educational technologies, Virtual Labs (V-Labs) have emerged as a promising solution, fundamentally altering how learners engage with scientific concepts and experiments. Despite their potential, ensuring their effectiveness and inclusivity in terms of accessibility to diverse students remains a challenging task. Currently, there is limited insight into the accessibility of V-Labs, a gap that our study aims to address. This study seeks to ascertain the effectiveness of V-Labs in terms of accessibility and inclusivity. We synthesized empirical studies, reviewing 36 articles published between 2000 and 2023. Of these articles, 69% of the studies were conducted in higher education and covered a wide range of learning environments. Our study revealed that 47.3% of the studies focused on various engineering subjects. Our findings provide insight into V-Labs’ accessibility from four key perspectives: (a) students with limited abilities, (b) diverse cultural and linguistic backgrounds, (c) instructional design features and content availability, and (d) interaction supporting features. We also identified existing gaps in the accessibility of the V-Labs from the four perspectives. Furthermore, we examined the assessment methods of V-Labs, shed light on the aspects that are evaluated, and underscored the need for future work on assessment strategies.
Shaping future-ready graduates with mindset shifts: studying the impact of integrating critical and design thinking in design innovation education
Authors: Patel Nadya Shaznay , Puah Shermain , Kok Xiao-Feng Kenan
Published: June 2024

In an era marked by rapid change and complex global challenges, Institutes of Higher Learning (IHLs) are tasked with preparing students to navigate and address these evolving demands. This paper explores the critical role of Higher Education (HE) in equipping students with the necessary skills and mindsets to tackle real-world problems through innovative solutions. Integrating critical thinking and design thinking within a Design Innovation module is central to this exploration. The study is undergirded by a conceptual framework that blends critical, design, and futures thinking, focusing exclusively in this paper on applying critical thinking (CT) and design thinking (DT). The research investigates two primary questions: (1) How do students’ DT and CT mindsets change after participation in a Design Innovation module? (2) Is CT a prerequisite for developing DT? This study aims to illuminate the shifts in students’ mindsets from before to after the completion of the module, highlighting the importance of developing key dispositions for ethical and socially responsible problem-solving. Results show a statistically significant increase in CT and DT disposition scores from pre- to post-test, suggesting a shift to more positive CT and DT mindsets after going through the Design Innovation module. In addition, a significant moderation effect of pre-test CT mindset on the relationship between pre-test and post-test DT mindset scores was observed, implying that CT was a prerequisite for DT. The findings offer insights into the module’s effectiveness in fostering future-ready graduates’ thinking capabilities on innovating for real-world challenges and highlight the need for our future-ready students to achieve critical competence and creative confidence. Finally, we conclude the paper with recommendations for educators to integrate CT skill development intentionally and in tandem with DT skill development for a balanced approach to developing critical competence and creative confidence in interdisciplinary courses.
Disruptive effects of plasticizers bisphenol A, F, and S on steroidogenesis of adrenocortical cells
Authors: Pötzl Benedikt , Kürzinger Lydia , Kendl Sabine , Stopper Helga , Kurlbaum Max , Fassnacht Martin , Dischinger Ulrich
Published: June 2024

Introduction

Endocrine disrupting chemicals (EDCs) are known to interfere with endocrine homeostasis. Their impact on the adrenal cortex and steroidogenesis has not yet been sufficiently elucidated. This applies in particular to the ubiquitously available bisphenols A (BPA), F (BPF), and S (BPS).

Methods

NCI-H295R adrenocortical cells were exposed to different concentrations (1nM-1mM) of BPA, BPF, BPS, and an equimolar mixture of them (BPmix). After 72 hours, 15 endogenous steroids were measured using LC-MS/MS. Ratios of substrate and product of CYP-regulated steps were calculated to identify most influenced steps of steroidogenesis. mRNA expression of steroidogenic enzymes was determined by real-time PCR.

Results

Cell viability remained unaffected at bisphenol concentrations lower than 250 µM. All tested bisphenols and their combination led to extensive alterations in the quantified steroid levels. The most profound fold changes (FC) in steroid concentrations after exposure to BPA (>10µM) were seen for androstenedione, e.g. a 0.37±0.11-fold decrease at 25µM (p≤0.0001) compared to vehicle-treated controls. For BPF, levels of 17-hydroxyprogesterone were significantly increased by 25µM (FC 2.57±0.49, p≤0.001) and 50µM (FC 2.65±0.61, p≤0.0001). BPS treatment led to a dose-dependent decrease of 11-deoxycorticosterone at >1µM (e.g. FC 0.24±0.14, p≤0.0001 at 10µM). However, when combining all three bisphenols, additive effects were detected: e.g. 11-deoxycortisosterone was decreased at doses >10µM (FC 0.27±0.04, p≤0.0001, at 25µM), whereas 21-deoxycortisol was increased by 2.92±0.20 (p≤0.01) at 10µM, and by 3.21±0.45 (p≤0.001) at 50µM. While every measured androgen (DHEA, DHEAS, androstenedione, testosterone, DHT) was lowered in all experiments, estradiol levels were significantly increased by BPA, BPF, BPS, and BPmix (e.g. FC 3.60±0.54, p≤0.0001 at 100µM BPF). Calculated substrate-product ratios indicated an inhibition of CYP17A1-, and CYP21A2 mediated conversions, whereas CYP11B1 and CYP19A1 showed higher activity in the presence of bisphenols. Based on these findings, most relevant mRNA expression of CYP genes were analysed. mRNA levels of StAR, CYP11B1, and CYP17A1 were significantly increased by BPF, BPS, and BPmix.

Discussion

In cell culture, bisphenols interfere with steroidogenesis at non-cytotoxic levels, leading to compound-specific patterns of significantly altered hormone levels. These results justify and call for additional in-vivo studies to evaluate effects of EDCs on adrenal gland functionality.
The association of platelet to white blood cell ratio with diabetes: a nationwide survey in China
Authors: Liu Fanglin , Wang Tianhong , Wang Siman , Zhao Xiumei , Hua Yusi
Published: June 2024

Background

Inflammation is integral to diabetes pathogenesis. The novel hematological inflammatory biomarker, platelet to white blood cell ratio (PWR), is linked with various conditions such as chronic kidney disease and stroke. However, the association of this novel clinical indicator with diabetes still remains unclear, which is investigated in this study.

Materials and Methods

A total of 10,973 Chinese participants were included and grouped according to the tertiles of PWR (T1, T2, and T3 groups). Diagnosis of prediabetes and diabetes adhered to American Diabetes Association criteria. Binary logistic regression was adopted to assess the relationship between PWR and both diabetes and prediabetes. The dose-response relationship of PWR and diabetes was examined using restricted cubic spline regression. Subgroup and interaction analyses were conducted to investigate potential covariate interactions.

Results

Individuals with higher PWR had better lifestyles and lipid profiles (all P < 0.05). After adjusting for all the covariates, the T2 group had a 0.83-fold (95% CI: 0.73–0.93, P < 0.01) risk of diabetes and that for the T3 group was 0.68-fold (95% CI: 0.60–0.78. P < 0.001). Dose-response analysis identified non-linear PWR-diabetes associations in the general population and females (both P < 0.05), but absent in males. Participants with prediabetes in the T2 and T3 groups had lower risks of diabetes (OR = 0.80 for the T2 group, P < 0.001 and 0.68 for the T3 group, P < 0.001) in the full models. All the sensitivity analysis support consistent conclusions.

Conclusions

An increase in PWR significantly correlates with reduced diabetes risks. A non-linear PWR-diabetes relationship exists in the general population and females, but not in males. The correlation between PWR and diabetes indicates that PWR holds potentials in early identification and prevention of diabetes.
Pine weevil (Hylobius abietis) preferences among species of conifer seedlings planted on clear-cuts in central Europe
Authors: Dvořáková Barbora , Holuša Jaroslav , Horák Jakub , Hradecký Jaromír , Bledý Michal , Zelenka Martin
Published: June 2024

Introduction

As a result of climate change and bark beetle outbreaks in forests, extensive salvage, and sanitary cutting have been undertaken in Europe, resulting in many clear-cuts with coarse stumps. The populations of pine weevils (Hylobius abietis and Hylobius pinastri) are steadily increasing. A high abundance of pine weevils feeding on seedlings typically results in significant economic losses and prolongs the forest establishment period. However, information on these species spatial distribution and habitat selection patterns, necessary for assessing their harmfulness and subsequently accurately estimating their threat to reforested areas, is lacking. To determine which factors influence spatial selection patterns and seedling type preference, this study investigated which clear-cut factors increase pine weevil abundance and which seedling types (species and age) are the most preferred.

Methods

The experiment was carried out on 20 clear-cuts in the central Czech Republic. We evaluated soil moisture level, average stump distance and diameter, proportion of other conifers, and whether the stumps were mulched. We detected the abundance of pine weevils using pitfall traps. We determined the feeding scar intensity on the first 10 cm of seedling stems on commonly used seedlings for reforestation in central Europe: 1-year-old Scots pine (Pinus sylvestris), European larch (Larix decidua), and 3-year-old Norway spruce (Picea abies), with 1,200 seedlings in total. The individual seedling types alternated in rows. The results were evaluated using generalized linear mixed-effect models (GLMMs). As dependent variables we used total numbers of H. abietis and H. pinastri. The soil moisture level, mulching, proportion of other conifers, average stump diameter, and distance were considered independent variables.

Results

We found that Norway spruce was the least attractive to pine weevils. We found a higher abundance of H. abietis females in moist clear-cuts, confirming that dry clear-cuts are less suitable for oviposition.

Conclusion

According to our findings, if foresters want to plant coniferous seedlings, it seems that planting older spruce is a better option than planting larch and pine. These findings provide valuable insights for forest management and reforestation strategies, equipping foresters with the knowledge to mitigate the threat of pine weevils and ensure successful forest establishment.
How does the age of control individuals hinder the identification of target genes for Huntington’s disease?
Authors: Dias Pinto João Rafael , Faustinoni Neto Benedito , Sanches Fernandes Joyce Macedo , Kerkis Irina , Araldi Rodrigo Pinheiro
Published: June 2024

Several studies have compared the transcriptome across various brain regions in Huntington’s disease (HD) gene-positive and neurologically normal individuals to identify potential differentially expressed genes (DEGs) that could be pharmaceutical or prognostic targets for HD. Despite adhering to technical recommendations for optimal RNA-Seq analysis, none of the genes identified as upregulated in these studies have yet demonstrated success as prognostic or therapeutic targets for HD. Earlier studies included samples from neurologically normal individuals older than the HD gene-positive group. Considering the gradual transcriptional changes induced by aging in the brain, we posited that utilizing samples from older controls could result in the misidentification of DEGs. To validate our hypothesis, we reanalyzed 146 samples from this study, accessible on the SRA database, and employed Propensity Score Matching (PSM) to create a “virtual” control group with a statistically comparable age distribution to the HD gene-positive group. Our study underscores the adverse impact of using neurologically normal individuals over 75 as controls in gene differential expression analysis, resulting in false positives and negatives. We conclusively demonstrate that using such old controls leads to the misidentification of DEGs, detrimentally affecting the discovery of potential pharmaceutical and prognostic markers. This underscores the pivotal role of considering the age of control samples in RNA-Seq analysis and emphasizes its inclusion in evaluating best practices for such investigations. Although our primary focus is HD, our findings suggest that judiciously selecting age-appropriate control samples can significantly improve best practices in differential expression analysis.
Single cell RNA-sequencing in uveal melanoma: advances in heterogeneity, tumor microenvironment and immunotherapy
Authors: Tang Shiyi , Zhang Yun , Huang Shengmei , Zhu Tengfei , Huang Xiaojing
Published: June 2024

Uveal melanoma (UM) is a highly aggressive and fatal tumor in the eye, and due the special biology of UM, immunotherapy showed little effect in UM patients. To improve the efficacy of immunotherapy for UM patients is of great clinical importance. Single-cell RNA sequencing(scRNA-seq) provides a critical perspective for deciphering the complexity of intratumor heterogeneity and tumor microenvironment(TME). Combing the bioinformatics analysis, scRNA-seq could help to find prognosis-related molecular indicators, develop new therapeutic targets especially for immunotherapy, and finally to guide the clinical treatment options.
The causal relationship between rheumatoid arthritis and bronchiectasis: a bidirectional Mendelian randomization study
Authors: Li Yuanyuan , Wang Weina , Zhou Dengfeng , Li Lili
Published: June 2024

Background

Several observational studies suggested an association between rheumatoid arthritis (RA) and bronchiectasis. Nevertheless, the presence of a causal relationship between these conditions is yet to be determined. This study aimed to investigate whether genetically predicted RA is associated with the risk of bronchiectasis and vice versa.

Methods

We obtained RA genome-wide association study (GWAS) data from FinnGen consortium, and bronchiectasis GWAS data from IEU Open GWAS project. Univariate Mendelian randomization (MR) analysis was performed using inverse variance weighted (IVW) estimation as the main method. Furthermore, bidirectional and replication MR analysis, multivariate MR (MVMR), Mediation analysis, and sensitivity analyses were conducted to validate the findings.

Results

In the UVMR analysis, the IVW results revealed that RA had an increased risk of bronchiectasis (OR = 1.18, 95% CI = 1.10–1.27; p = 2.34 × 10−6). In the reverse MR analysis, no evidence of a causal effect of bronchiectasis on the risk of RA was detected. Conversely, in the replication MR analysis, RA remained associated with an increased risk of bronchiectasis. Estimates remained consistent in MVMR analyses after adjusting for the prescription of non-steroidal anti-inflammatory drugs (NSAIDs) and glucocorticoids. Immunosuppressants were found to mediate 58% of the effect of the RA on bronchiectasis. Sensitivity analyses confirmed the stability of these associations.

Conclusion

This study demonstrated a positive causal relationship between RA and an increased risk of bronchiectasis, offering insights for the early prevention of bronchiectasis in RA patients and shedding new light on the potential role of immunosuppressants as mediators in promoting the effects of RA on bronchiectasis.
Exercise therapy in the application of sleep disorders
Authors: Zhao Yuhang , Dai Qiang , Li Yuhao , Li Chenyang
Published: June 2024

Sleep disorders often accompany neurological injuries, significantly impacting patient recovery and quality of life.The efficacy and adherence of traditional treatment methods have certain limitations. Exercise has been found to be a highly beneficial treatment method, capable of preventing and alleviating neurological injuries and sleep disorders. This article reviews relevant research findings from both domestic and international sources over the past few decades, systematically summarizing and analyzing the application of exercise therapy in sleep disorders,strategy of exercise intervention program and the potential molecular mechanisms by which exercise therapy improves sleep disorders. Shortcomings in current research and suggestions are presented, providing a reference for future in-depth studies on exercise interventions for sleep disorders.
Immediate and long-term electrophysiological biomarkers of antidepressant-like behavioral effects after subanesthetic ketamine and medial prefrontal cortex deep brain stimulation treatment
Authors: Bergosh Matthew , Medvidovic Sasha , Zepeda Nancy , Crown Lindsey , Ipe Jennifer , Debattista Lauren , Romero Luis , Amjadi Eimon , Lam Tian , Hakopian Erik , Choi Wooseong , Wu Kevin , Lo Jack Yu Tung , Lee Darrin Jason
Published: June 2024

Introduction

Both ketamine (KET) and medial prefrontal cortex (mPFC) deep brain stimulation (DBS) are emerging therapies for treatment-resistant depression, yet our understanding of their electrophysiological mechanisms and biomarkers is incomplete. This study investigates aperiodic and periodic spectral parameters, and the signal complexity measure sample entropy, within mPFC local field potentials (LFP) in a chronic corticosterone (CORT) depression model after ketamine and/or mPFC DBS.

Methods

Male rats were intraperitoneally administered CORT or vehicle for 21 days. Over the last 7 days, animals receiving CORT were treated with mPFC DBS, KET, both, or neither; then tested across an array of behavioral tasks for 9 days.

Results

We found that the depression-like behavioral and weight effects of CORT correlated with a decrease in aperiodic-adjusted theta power (5–10 Hz) and an increase in sample entropy during the administration phase, and an increase in theta peak frequency and a decrease in the aperiodic exponent once the depression-like phenotype had been induced. The remission-like behavioral effects of ketamine alone correlated with a post-treatment increase in the offset and exponent, and decrease in sample entropy, both immediately and up to eight days post-treatment. The remission-like behavioral effects of mPFC DBS alone correlated with an immediate decrease in sample entropy, an immediate and sustained increase in low gamma (20–50 Hz) peak width and aperiodic offset, and sustained improvements in cognitive function. Failure to fully induce remission-like behavior in the combinatorial treatment group correlated with a failure to suppress an increase in sample entropy immediately after treatment.

Conclusion

Our findings therefore support the potential of periodic theta parameters as biomarkers of depression-severity; and periodic low gamma parameters and cognitive measures as biomarkers of mPFC DBS treatment efficacy. They also support sample entropy and the aperiodic spectral parameters as potential cross-modal biomarkers of depression severity and the therapeutic efficacy of mPFC DBS and/or ketamine. Study of these biomarkers is important as objective measures of disease severity and predictive measures of therapeutic efficacy can be used to personalize care and promote the translatability of research across studies, modalities, and species.
Evaluation of acetic acid treatment of fresh–cut water chestnuts using gray–correlation analysis based on the variation–coefficient weight
Authors: Zhang Yongqing , Li Haiyan , Liu Jiangfan , Wei Quanzeng , Sun Juntao , Wang Deguo
Published: June 2024

Introduction

The demand for fresh–cut water chestnuts, a convenient and nutritive vegetable, is increasing in market. However, the slicing of water chestnuts can cause mechanical damage to tissue, which results in quality deterioration. We aimed to select the optimal treatment through a comprehensive comparison of the preservation effect of acetic acid, which could prolong the shelf life of fresh–cut water chestnuts and improve their storage quality.

Methods

A comprehensive evaluation was conducted using the gray–correlation method based on the variation–coefficient weight to observe the treatment of 0, 2 and 5% acetic acid. Their effects on color, weight loss rate, and the content of ascorbic acid, total sugar, reducing sugar, soluble protein, and free amino acid were determined.

Results

The color, weight loss rate, and nutritional content of fresh–cut chestnuts varied under different processing and storage times. When stored for more than 4 days, the b* value, and the content of total sugar and soluble protein in CK were higher than those in 2% or 5% acetic acid, but the weight loss rate, and the content of ascorbic acid and free amino acid in CK were less than those in acetic acid treatments. Considering various indicators, it was difficult to determine which treatment to choose for fresh–cut water chestnut preservation. The gray–correlation analysis results indicated that when stored for 8, 12, or 16 days, the gray–correlation degree of 5% acetic acid was the highest, while that of the control was the lowest. It could be directly concluded by the gray–correlation degree that when the storage time exceeded 4 days, acetic acid could be used to improve storage quality, and 5% acetic acid had a better preservation effect than 2%. Fresh–cut water chestnuts can be stored for 4 days without the need for acetic acid treatment.

Conclusion

These findings could provide information and comprehensive evaluation methods for the preservation of fresh–cut fruits and vegetables. The next step is to evaluate the preservation effect of acetic acid by measuring its effects on other indicators of fresh–cut water chestnuts (e.g., flavonoids, and microorganisms), providing ideas for the research of preservatives.
Toxicological safety assessment of a water extract of Lithocarpus litseifolius by a 90-day repeated oral toxicity study in rats
Authors: Ma Jinfeng , Wei Yujia , Sun Jingfeng , Tan Fang , Liu Penghe , Qin Guangqiu
Published: June 2024

Lithocarpus litseifolius although known as “Sweet Tea” (ST), has been traditionally accepted as a daily beverage and used as a folk medicine in southern China with little understanding of its potential toxicity. This study evaluated the safety of a water extract of ST by a subchronic toxicity study in Sprague-Dawley rats. A total of 80 rats were randomized divided into 4 groups with 10 males and 10 females in each group, treated with 2000, 1,000, 500 and 0 mg/kg body weight of ST extract by gavage for 90 days, respectively. The results of the study showed that ST extract did not induce treatment-related changes in the body and organ weight, food intake, blood hematology and serum biochemistry, urine indices, and histopathology in rats. The NOAEL of ST extract was observed to be 2000 mg/kg/day for rats of both sexes. These results indicated that ST extract was of low toxicity in the experimental conditions of the current study and had the potential for application in food-related products.
Bidirectional pressure: a mini review of ventilator-lung-kidney interactions
Authors: Kumar Avnee , Epler Katie , DeWolf Sean , Barnes Laura , Hepokoski Mark
Published: June 2024

Acute kidney injury and respiratory failure that requires mechanical ventilation are both common complications of critical illnesses. Failure of either of these organ systems also increases the risk of failure to the other. As a result, there is a high incidence of patients with concomitant acute kidney injury and the need for mechanical ventilation, which has a devasting impact on intensive care unit outcomes, including mortality. Despite decades of research into the mechanisms of ventilator-lung-kidney interactions, several gaps in knowledge remain and current treatment strategies are primarily supportive. In this review, we outline our current understanding of the mechanisms of acute kidney injury due to mechanical ventilation including a discussion of; 1) The impact of mechanical ventilation on renal perfusion, 2) activation of neurohormonal pathways by positive pressure ventilation, and 3) the role of inflammatory mediators released during ventilator induced lung injury. We also provide a review of the mechanisms by which acute kidney injury increases the risk of respiratory failure. Next, we outline a summary of the current therapeutic approach to preventing lung and kidney injury in the critically ill, including fluid and vasopressor management, ventilator strategies, and treatment of acute kidney injury. Finally, we conclude with a discussion outlining opportunities for novel investigations that may provide a rationale for new treatment approaches.
Spatial distribution and drivers of arbuscular mycorrhizal fungi on the Tibetan Plateau
Authors: Zhang Feng , Li Yaoming , Ji Baoming , Dong Shikui
Published: June 2024

Introduction

Arbuscular mycorrhizal fungi (AMF) are pivotal in plant resource acquisition, mediating plant interactions, and influencing soil carbon dynamics. However, their biogeographical distribution in Tibetan alpine grasslands remains understudied.

Methods

In this research, we examined the distribution pattern of AMF communities and their key determinants along a 2000-km transect across the Tibetan plateau, encompassing 7 alpine meadows and 8 alpine steppes.

Results

Our findings indicate that AMF community diversity and composition exhibit similarities between alpine meadows and alpine steppes, primarily influenced by latitude and evapotranspiration. At the genus level, Glomus predominated in both alpine meadow (36.49%±2.67%) and alpine steppe (41.87%±2.36%) soils, followed by Paraglomus (27.14%±3.69%, 32.34%±3.28%). Furthermore, a significant decay relationship of AMF community was observed over geographical distance. Null model analyses revealed that random processes predominantly (>50%) drove the assembly of AMF communities.

Discussion

In summary, our study elucidates the spatial distribution pattern of AMF in Tibetan plateau grasslands and underscores the significant influence of geographical and climatic factors on AMF community dynamics.
Downward social comparison positively promotes altruism: the multi-mediating roles of belief in a just world and general life satisfaction
Authors: Hu Yunjun , Cui Guanyu , Jiang Linxi , Lan Xiaoyu
Published: June 2024

Introduction

This study examined the underlying mechanism of the relationship between downward social comparison (DSC) and altruism through two conceptually important but rarely studied factors: belief in a just world (BJW) and general life satisfaction (GLS).

Methods

The study utilized a two-time-point design, spaced 2 months apart, with a sample of 1,764 college students from China. The study measured DSC, altruism, BJW, and GLS.

Results

The findings revealed a significant positive predictive effect of DSC on college students’ altruism and belief in a just world (BJW). Notably, GLS emerged as a multi-mediating factor in this relationship. The study also revealed that both subscales of BJW, namely personal belief in a just world (PBJW) and general belief in a just world (GBJW), played distinct roles in the mediation model. PBJW exhibited a stronger mediating effect, suggesting that DSC can foster individual altruism and BJW. Particularly, BJW was identified as enhancing GLS, subsequently promoting altruistic behavior.

Conclusion

This study contributes to the existing literature on social comparison by shedding light on the relationship between DSC, altruism, and the mediating role of belief in a just world and GLS. The findings underscore the potential for promoting altruistic behavior among college students through interventions targeting beliefs in a just world, especially at the personal level, and enhancing GLS.
Causal relationships between height, screen time, physical activity, sleep and myopia: univariable and multivariable Mendelian randomization
Authors: Liu Xiaoyu , Zhao Fangkun , Yuan Weichen , Xu Jun
Published: June 2024

Background

This study aims to investigate the independent causal relation between height, screen time, physical activity, sleep and myopia.

Methods

Instrumental variables (IVs) for exposures and outcome were obtained from the largest publicly available genome-wide association studies (GWAS) databases. First, we performed a bidirectional univariate MR analysis using primarily the inverse variance weighted method (IVW) with height, screen time, physical activity and sleep as the exposure and myopia as the outcome to investigate the causal relationship between exposures and myopia. Sensitivity analysis was used to demonstrate its robustness. Then the multivariable MR (MVMR) and MR-based mediation approach was further used to estimate the mediating effect of potential confounders (education and time outdoors) on causality.

Results

The results of univariate MR analysis showed that taller height (OR = 1.009, 95% CI = 1.005–1.012, p = 3.71 × 10−7), longer time on computer (OR = 1.048, 95% CI = 1.029–1.047, p = 3.87 × 10−7) and less moderate physical activity (OR = 0.976, 95% CI = 0.96–0.991 p = 2.37 × 10−3) had a total effect on the increased risk of developing myopia. Meanwhile our results did not have sufficient evidence to support the causal relationship between chronotype (p = 0.637), sleep duration (p = 0.952) and myopia. After adjusting for education, only taller height remains an independent risk factor for myopia. After adjusting for education, the causal relationship between height, screen and myopia still had statistical significance. A reverse causal relationship was not found in our study. Most of the sensitivity analyses showed consistent results with those of the IVW method.

Conclusion

Our MR study revealed that genetically predicted taller height, longer time on computer, less moderate physical activity increased the risk of myopia. After full adjustment for confounders, only height remained independently associated with myopia. As a complement to observational studies, the results of our analysis provide strong evidence for the improvement of myopia risk factors and provide a theoretical basis for future measures to prevent and control myopia in adolescents.
Sensor Fusion Architecture for Fault Diagnosis with a Predefined-Time Observer
Authors: Begovich Ofelia , Lizárraga Adrián , Ramírez-Treviño Antonio
Published: June 2024

This study focuses on generating reliable signals from measured noisy signals through an enhanced sensor fusion method. The main contribution of this research is the development of a novel sensor fusion architecture that creates virtual sensors, improving the system’s redundancy. This architecture utilizes an input observer to estimate the system input, then it is introduced to the system model, the output of which is the virtual sensor. Then, this virtual sensor includes two filtering stages, both derived from the system’s dynamics—the input observer and the system model—which effectively diminish noise in the virtual sensors. Afterwards, the same architecture includes a classical sensor fusion scheme and a voter to merge the virtual sensors with the real measured signals, enhancing the signal reliability. The effectiveness of this method is shown by applying merged signals to two distinct diagnosers: one utilizes a high-order sliding mode observer, while the other employs an innovative extension of a predefined-time observer. The findings indicate that the proposed architecture improves diagnostic results. Moreover, a three-wheeled omnidirectional mobile robot equipped with noisy sensors serves as a case study, confirming the approach’s efficacy in an actual noisy setting and highlighting its principal characteristics. Importantly, the diagnostic systems can manage several simultaneous actuator faults.
Towards Sustainable Inventory Management: A Many-Objective Approach to Stock Optimization in Multi-Storage Supply Chains
Authors: Santos João A. M. , Martins Miguel S. E. , Pinto Rui M. , Vieira Susana M.
Published: June 2024

Within the framework of sustainable supply chain management and logistics, this work tackles the complex challenge of optimizing inventory levels across varied storage facilities. It introduces a comprehensive many-objective optimization model designed to minimize holding costs, energy consumption, and shortage risk concurrently, thereby integrating sustainability considerations into inventory management. The model incorporates the distinct energy consumption profiles associated with various storage types and evaluates the influence of stock levels on energy usage. Through an examination of a 60-day production schedule, the dynamic relationship between inventory levels and operational objectives is investigated, revealing a well-defined set of optimal solutions that highlight the trade-off between energy savings and shortage risk. Employing a 30-day rolling forward analysis with daily optimization provides insights into the evolving nature of inventory optimization. Additionally, the model is extended to encompass a five-objective optimization by decomposing shortage risk, offering a nuanced comprehension of inventory risks. The outcomes of this research provide a range of optimal solutions, empowering supply chain managers to make informed decisions that strike a balance among cost, energy efficiency, and supply chain resilience.
A Lightweight Crop Pest Classification Method Based on Improved MobileNet-V2 Model
Authors: Peng Hongxing , Xu Huiming , Shen Guanjia , Liu Huanai , Guan Xianlu , Li Minhui
Published: June 2024

This paper proposes PestNet, a lightweight method for classifying crop pests, which improves upon MobileNet-V2 to address the high model complexity and low classification accuracy commonly found in pest classification research. Firstly, the training phase employs the AdamW optimizer and mixup data augmentation techniques to enhance the model’s convergence and generalization capabilities. Secondly, the Adaptive Spatial Group-Wise Enhanced (ASGE) attention mechanism is introduced and integrated into the inverted residual blocks of the MobileNet-V2 model, boosting the model’s ability to extract both local and global pest information. Additionally, a dual-branch feature fusion module is developed using convolutional kernels of varying sizes to enhance classification performance for pests of different scales under real-world conditions. Lastly, the model’s activation function and overall architecture are optimized to reduce complexity. Experimental results on a proprietary pest dataset show that PestNet achieves classification accuracy and an F1 score of 87.62% and 86.90%, respectively, marking improvements of 4.20 percentage points and 5.86 percentage points over the baseline model. Moreover, PestNet’s parameter count and floating-point operations are reduced by 14.10% and 37.50%, respectively, compared to the baseline model. When compared with ResNet-50, MobileNet V3-Large, and EfficientNet-B1, PestNet offers superior parameter efficiency and floating-point operation requirements, as well as improved pest classification accuracy.
Optimization of Hairy Root Transformation and Application of RUBY as a Reporter in Lotus corniculatus
Authors: Lyu Kaidi , Zhang Xingli , Yu Wenjie , Lyu Shanhua , Fan Yinglun
Published: June 2024

Lotus corniculatus is an important perennial legume forage species and has been widely used to study the relationships between plants and rhizobia or arbuscular mycorrhizal fungi. Composite plants (wild-type shoots with transgenic hairy roots) can be produced via genetic transformation mediated by Agrobacterium rhizogenes. The A. rhizogenes puncture method and a method of cutting embryonic roots and coating the hypocotyl incision with A. rhizogenes were used to induce hairy roots from seedlings. The identification of transgenic hairy roots from composite plants is also an important task. Here, young branches from adult plants were used as explants to produce composite plants instead of seedlings via the one-step cutting method in the natural environment. The results showed that the explants of young branches had higher transformation frequency than those of seedlings, and the composite plants obtained from the young branches had greater growth and were also more robust than the plants obtained from the seedlings. In this study, a system for the biosynthesis of betalains named RUBY was integrated into an expression vector as a reporter gene. It is very easy to distinguish transgenic roots from nontransgenic roots based on the red color of the hairy roots. Young branches were used as explants for hairy root transformation with higher transformation frequency than the seedlings. RUBY was used as a reporter gene to distinguish transgenic roots from nontransgenic roots based on the red color of the hairy roots.
Relationships and Changes in Grassland Community Diversity and Biomass in the Pastoral Areas of the Two Rivers under Grazing Disturbance
Authors: Chen Weilong , Ye Mao , Pan Xiaoting , Li Miaomiao , Zeng Guoyan , Zhang Xi , He Qingzhi , Gu Xinchen , Qian Jiaorong , Lv Yexin , Che Jing
Published: June 2024

Grazing affects plant community characteristics and the relationship between above-ground biomass and diversity, which has become a hot topic in grassland ecosystem research in recent years. The present study investigated grassland vegetation in the Two Rivers pastoral area based on 60 sampling points using sampling methods. The effects of grazing on the structural characteristics of plant communities were analysed using the α-diversity index, and the relationship between species diversity and the above-ground biomass of plant communities, as well as their changes, was explored. The results showed that grazing disturbance decreased the number of family species in the grassland plant communities and the similarity between communities; grazing activities significantly decreased the above-ground biomass of the grassland plant communities; the height and cover also showed a decreasing trend (p < 0.01), but the density of the grassland plant communities improved (p < 0.01). The Margalef index, the Shannon–Wiener index, and the Simpson index of the grassland plant communities all showed different degrees of decrease after grazing, while the Alatalo index showed an increase; biomass was positively correlated with the Margalef, Shannon–Wiener, and Alatalo indices and negatively correlated with the Simpson index (p < 0.01). Therefore, in the management and maintenance of grasslands, the impact of grazing on the structural characteristics of plant communities should be taken into account, and reasonable response policies should be implemented according to the actual local situation. This study provides a theoretical basis for grassland maintenance and management and the sustainable development of ecosystems in the pastoral areas of the two river sources of the Altay Mountains.
Characterization and Association of the Missing Ventral Tubercle(s) from the Sixth Cervical Vertebra and Transpositions on the Ventral Surface of the Seventh Cervical Vertebra in Modern Equus ferus caballus
Authors: May-Davis Sharon , Eckelbarger Pamela Blades , Dzingle Diane , Saber Elle
Published: June 2024

In recent years, equine complex vertebral malformation (ECVM) has been of concern in the equine community, with studies identifying numerous associative morphological variations. Here, we examine the morphological association between C6 and C7 for dependency in ECVM cases, where the partially absent ventral process of C6 transposes on the ventral surface of C7. A C6 ventral process presents two tubercles, one cranial (CrVT) and one caudal (CVT). In this study, the C6 osseous specimens (n = 85) demonstrated a partial or completely absent CVT (aCVT) graded 1–4 that often extended cranially creating a partially absent cranial ventral tubercle (aCrVT) graded 1–3. In the 85 C6 osseous specimens examined, the corresponding C7s demonstrated either a complete or incomplete transposition of the ventral process from C6 in 44/85, with 30/44 replicating a transverse foramen. A strong statistical dependency existed between C6 grade 4 aCVTs and grades 1–3 aCrVTs and C7 transpositions with replicated transverse foramen. Sidedness was also demonstrated, where a left sided absent C6 associated with transposition on the left ventral surface of C7. This likewise applied to right sidedness and most bilateral cases. These findings might benefit practitioners when radiographing the extent of the ECVM configuration in patients presenting caudal cervical pain.
Fiber Lidar for Control of the Ecological State of the Atmosphere
Authors: Volkov Sergei N. , Zaitsev Nikolai G. , Park Sun-Ho , Kim Duk-Hyeon , Noh Young-Min
Published: June 2024

Methods and means of remote control of the ecological state of the atmosphere are constantly improving. Lidar sensing allows obtaining up-to-date information about natural and technogenic sources of atmospheric pollution. There is a wide range of problems in ecological control, where the deployment of an inexpensive mobile lidar network is required. For this purpose, it is suggested to use Q-switch and MOPA fiber lasers in lidars. Q-switch fiber lasers have a simpler design and are more practical to use. However, pulses from Q-switch lasers have long full-pulse durations. In the present work, a lidar signal inversion method (LSIM) is proposed for solving this problem. Verification and outdoor experimentation of the LSIM was carried out with the reference signal method (RSM). The advantage of the proposed RSM is the minimum number of controllable parameters necessary for LSIM verification and approbation. As a result, the accuracy of the obtained results increased. Thus, the possibility of application of the Q-switch fiber lasers for lidar sensing is shown both theoretically and experimentally.