bluuebunny/crossref_metadata_2025 huggingface dataset:
Nascent Chains Derived from a Foldable Protein Sequence Interact with Specific Ribosomal Surface Sites near the Exit Tunnel
Authors: Masse Meranda M. , Guzman-Luna Valeria , Varela Angela E. , Hutchinson Rachel B. , Srivast Aniruddha , Wei Wanting , Fuchs Andrew M. , Cavagnero Silvia
Published: April 2023

In order to become bioactive, proteins must be translated and protected from aggregation during biosynthesis. The ribosome and molecular chaperones play a key role in this process. Ribosome-bound nascent chains (RNCs) of intrinsically disordered proteins and RNCs bearing a signal/arrest sequence are known to interact with ribosomal proteins. However, in the case of RNCs bearing foldable protein sequences, no direct information is available on these interactions. Here, via a combination of chemical crosslinking and time-resolved fluorescence-anisotropy, we find that nascent chains of the foldable globin apoHmp1 − 140 interact with ribosomal protein L23 and have a freely-tumbling non-interacting N-terminal compact region comprising 63–94 residues. Longer RNCs (apoHmp1 − 189) also interact with an additional yet unidentified ribosomal protein, as well as with chaperones. Surprisingly, the apparent strength of RNC/r-protein interactions does not depend on nascent-chain sequence. Overall, foldable nascent chains establish and expand interactions with selected ribosomal proteins and chaperones, as they get longer. These data are significant because they reveal the interplay between independent conformational sampling and nascent-protein interactions with the ribosomal surface.
Effectiveness of biological nurturing on maternal breastfeeding problems: a systematic review and meta-analysis.
Authors: hu longyi , zhou leshan , zhou ziyun
Published: April 2023

Background Breastfeeding is important for maternal and child health, yet the rate of exclusive breastfeeding for the first 6 months after birth is low worldwide. Feeding posture is one of the factors that affect breastfeeding. Several articles on biological nurturing have shown that It can improve breastfeeding, but the evaluation metrics of these articles vary and no uniform standards.Research Aims To investigate the influence of biological nurturing on breastfeeding.Method Cochrane library, Embase, PubMed, web of science, Medline, EBSCO, CBM, CNKI, VIP, WANFANG were systematically searched for studies that met criteria. Two investigators independently assessed the quality of the selected literature and screened data; 14 studies involving 2547 participants were included and meta-analysis was performed using revman 5.3 and state15.1.Results The results showed that the observation group compared to the control group in terms were statistically different, the incidence of exclusive breastfeeding [RR = 1.16,95%CI=[1.10,1.23],P < 0.001], the incidence of breastfeeding success [RR = 1.23,95%CI=[1.13,1.33],P < 0.001], maternal satisfaction [effect size = 1.22,95%CI=[1.16, 1.28],P < 0.001], mental status scale in non-psychiatric settings (MSSNS) score [SMD=-3.05,95%CI=[-3.44,-2.65],P < 0.001], breast pain condition [RR = 0.72.95%CI=[0.61, 0.85],P < 0.001], breastfeeding self-efficacy [SMD = 0.69,95%CI [0.09,1.29]=,P = .02],lactation initiation time[SMD=-1.50, 95%CI=[-2.65, -0.36],P = .01], and postural comfort [SMD = 0.85, 95%CI=[0.68, 1.02],P < 0.001], indicating that the biological nurturing method had a positive effect.Conclusions: Biological nurturing has been shown to better facilitate postpartum women in increasing exclusive breastfeeding incidence, the chance of successful breastfeeding, improving maternal psychological state, maternal satisfaction, maternal breastfeeding self-efficacy and reducing breast pain and breast injury, compared to traditional breastfeeding positions. There is little research on the effects of biologic nurturing on infants, which could be further explored.
Extensive freshened groundwater resources emplaced during the Messinian sea-level drawdown: Insights from southern Sicily (Italy)
Authors: Lipparini Lorenzo , Chiacchieri Damiano , Bencini Roberto , Micallef Aaron
Published: April 2023

Deep groundwater resources around the world represent an important potential unconventional source of water. Here we document an extensive (17.3 km3) fresh/brackish groundwater body preserved in a deep (between 800 and 2100 m) carbonate platform aquifer in southern Sicily (Italy), by using deep well data and a 3D hydrogeological modelling. We attribute the distribution of this fossil groundwater to topographically-driven meteoric recharge driven by the Messinian sea-level drawdown, which we estimate to have reached 2400 m below present sea level in the eastern Mediterranean Basin. The discovery of such an extensive and deep freshened groundwater has significant implications in terms of resource potential for southern Sicily and other Mediterranean coastal regions, that share similar geological setting and water scarcity issues.
Capicua (CIC) mutations in gliomas in association with MAPK activation for exposing a potential therapeutic target
Authors: Darabi Sourat , Xiu Joanne , Samec Timothy , Kesari Santosh , Carrillo Jose , Aulakh Sonikpreet , Walsh Kyle M. , Sengupta Soma , Sumrall Ashley , Spetzler David , Glantz Michael , Demeure Michael J
Published: April 2023

Gliomas are the most prevalent neurological cancer in the United States and care modalities are not able to effectively combat these aggressive malignancies. Identifying new, more effective treatments require a deep understanding of the complex genetic variations and relevant pathway associations behind these cancers. Drawing connections between gene mutations with a responsive genetic target can help drive therapy selections to enhance patient survival. We have performed extensive molecular profiling of the Capicua gene (CIC), a tumor and transcriptional suppressor gene, and its mutation prevalence in reference to MAPK activation within clinical glioma tissue. CIC mutations occur far more frequently in oligodendroglioma (52.1%) than in low-grade astrocytoma or glioblastoma. CIC-associated mutations were observed across all glioma subtypes, and MAPK-associated mutations were most prevalent in CIC wild-type tissue regardless of the glioma subtype. MAPK activation, however, was enhanced in CIC-mutated oligodendroglioma. The totality of our observations reported supports the use of CIC as a relevant genetic marker for MAPK activation. Identification of CIC mutations, or lack thereof, can assist in selecting, implementing, and developing MEK/MAPK-inhibitory trials to improve patient outcomes potentially.
Revision Anterior Cruciate Ligament Reconstruction with Over-the-top Augmentation Technique Using an Achilles Tendon Allograft for Enhanced Rotational Stability
Authors: Lim Sumin , Park Ki-Hoon , Park Do Young , Kim Tae Hun , Koh Jeong-Hyun , Chung Jun Young
Published: April 2023

Purpose
Revision anterior cruciate ligament (ACL) reconstruction is technically challenging due to mispositioned tunnels, bone loss, and tunnel expansion, which may compromise graft fixation or strength and result in failure. To obtain firm graft fixation and strength in one stage, we developed a new over-the-top augmentation technique using an Achilles tendon allograft in revision ACL reconstruction (OA-ACLR). This study compared OA-ACLR with single-bundle ACL reconstruction (SB-ACLR). We hypothesized that OA-ACLR would enhance the postoperative knee joint rotational stability.
Methods
We retrospectively analyzed 47 patients who underwent revisional OA-ACLR and 48 who underwent primary SB-ACLR. Knee instability was evaluated with the anterior drawer, Lachman, and pivot shift tests preoperatively and at the final follow-up. Side-to-side differences were compared with the non-affected side at the final follow-up. Function was evaluated using the IKDC subjective and Lysholm knee scores preoperatively and at the final follow-up.
Results
The groups did not differ in terms of sex, age, and BMI. Both groups significantly improved in the anterior drawer, Lachman, pivot shift tests, and IKDC and Lysholm knee scores after surgery (all p < 0.001). The OA-ACLR group showed significantly higher rotational stability in the pivot shift test than the SB-ACLR group (p = 0.017). The postoperative side-to-side difference, the IKDC and Lysholm scores showed no significant differences between the groups (p = 0.34, 0.301, 0.438).
Conclusions
OA-ACLR can yield a firm, stable graft reconstruction and guarantee one-stage reconstruction regardless of bony tunnel conditions. This technique can contribute to enhanced knee rotational stability and may be considered a useful alternative for revision anterior cruciate ligament reconstruction.
Trade-offs and synergistic relationships of ecosystem services in the Qinling mountains, China
Authors: Zhao Yonghua , Kang Shuaizhi , Yan Jin , Huang Xin , Zhang Yang , Wang Huanyuan , Liu Jinbao
Published: April 2023

To accelerate the construction of the national ecological security pattern and achieve the mutual promotion of regional ecological protection and economic development, this study focuses on the Qinling Mountains as the study area. Remote sensing, meteorological, and soil data were used to assess habitat quality, soil and water conservation, water production, and water purification trade-offs in the study area from 1980 to 2018. The study also explored the synergistic relationships among different ecosystem services and their spatial and temporal changes. The main findings revealed an overall increasing trend in habitat quality from 1980 to 2018, while soil and water conservation displayed an overall decreasing trend. Water production also showed an overall decreasing trend, while the maintenance of nitrogen and phosphorus both showed a decreasing trend. The study found a weak trade-off between habitat quality and soil conservation and water production, and a strong synergistic relationship with water quality purification. The study also revealed strong synergistic relationships between soil and water conservation and water production, weaker synergistic relationships between water production and water purification, and a stronger synergistic relationship between water production and water purification.
Identification of Soybean Genes Correlated with Rhizobial Nitrogen Fixation Activity
Authors: Yano Shunichi , Saeki Yuichi
Published: April 2023

Background and AimsSoybean (Glycine maxL.) has the ability to establish a symbiotic relationship with rhizobia. However, little is known about the host plant genes that correlate with the nitrogen fixation activity of rhizobia.MethodsHerein, soybean seeds were inoculated with two strains of wild-type soybean rhizobia with different nitrogen fixation activities and grown in the field until the R1 stage.ResultsThereafter, the roots and nodules were subjected to RNA-Seq to detect the expression of soybean genes related to nitrogen fixation, followed by the analysis of the community structure of the soybean-nodulating rhizobia.GmNRT2.5, a high-affinity nitrate transporter gene, was correlated with nitrogen fixation activity. Real-time PCR analysis of the roots and nodules inoculated with a nod+/fix-(Δnifgenes) mutant rhizobia showed thatGmNRT2.5on chromosome 8 (Glyma.08G284000:GmNRT2.5Chr8) was especiallycorrelated with nitrogen fixation activity.ConclusionGmNRT2.5Chr8 acts as a switching strategy between nitrogen fixation and acquisition from the soil. We also found thatGmNRT2.5Chr8 is a useful host gene that acts as an indicator of nitrogen fixation activity.
Reliability and Validity of the Chinese Version of the Achievement Emotions Questionnaire for Physical Education in University Students
Authors: Tian Jianing , Liu Peifeng , Zhang Qianqian , Song Shun , An Shicheng , Yu Hongyan
Published: April 2023

BackgroundAchievement emotions have a significant impact on both the learning process and outcomes. However, there is currently no brief and effective questionnaire available to evaluate Chinese university students' achievement emotions in physical education courses. This study aimed to examine the reliability and validity of the Achievement Emotions for Physical Education Questionnaire (AEQ-PE) in a sample of Chinese university students, while also investigating its measurement invariance across gender and grade levels.MethodsA cluster randomization sampling method was used to select 694 first- and second-year university students in Shanghai, China for the survey. Descriptive statistics, item analysis, reliability testing, and measurement invariance testing were conducted on the full sample (n = 694). Subsequently, the full sample was randomly divided into two groups, with Sample 1 (n = 347) undergoing exploratory factor analysis (EFA), and Sample 2 (n = 347) undergoing confirmatory factor analysis (CFA) to test the structural validity, convergent validity, and discriminant validity of the Chinese version of the Achievement Emotions Questionnaire for Physical Education (AEQ-PE-C). Finally, Sample 3 (n = 45), which was retested one month later, was used to evaluate test-retest reliability.ResultsThe Chinese version of the Achievement Emotions Questionnaire for Physical Education consists of 6 dimensions and 24 items, with good item discrimination. The EFA supported a 6-factor structure model, while the CFA demonstrated good model fit indices (χ2/df = 3.086, CFI = 0.928, TLI = 0.916, RMSEA = 0.078) and good convergent and discriminant validity. The questionnaire exhibits high internal consistency reliability (0.794) and excellent test-retest reliability (0.792). Furthermore, the multi-group analysis confirms that the AEQ-PE-C questionnaire has measurement invariance across gender and grade levels.ConclusionThe Chinese version of the Achievement Emotions Questionnaire for Physical Education has good reliability and validity, as well as measurement invariance across gender and grade levels, making it an effective tool for measuring achievement emotions in physical education among Chinese university students.
New insights into the responder/nonresponder divide in rectal cancer: Damage-induced Type I IFNs dictate treatment efficacy and can be targeted to enhance radiotherapy
Authors: Gerber Scott , Uccello Taylor , Lesch Maggie , Kintzel Sarah , Gradzewicz Lauren , Lamrous Lillia , Murphy Shawn , Fleming Fergal , Mills Bradley , Murphy Joseph , Hughson Angela , Garrett-Larsen Jesse , Qiu Haoming , Drage Michael , Ye Jian , Gavras Nicholas , Keeley David , Love Tanzy , Repasky Elizabeth , Lord Edith , Linehan David
Published: April 2023

Rectal cancer ranks as the second leading cause of cancer-related deaths. Neoadjuvant therapy for rectal cancer patients often results in individuals that respond well to therapy and those that respond poorly, requiring life-altering excision surgery. It is inadequately understood what dictates this responder/nonresponder divide. Our major aim is to identify what factors in the tumor microenvironment drive a fraction of rectal cancer patients to respond to radiotherapy. We also sought to distinguish potential biomarkers that would indicate a positive response to therapy and design combinatorial therapeutics to enhance radiotherapy efficacy. To address this, we developed an orthotopic murine model of rectal cancer treated with short course radiotherapy that recapitulates the bimodal response observed in the clinic. We utilized a robust combination of transcriptomics and protein analysis to identify differences between responding and nonresponding tumors. Our mouse model recapitulates human disease in which a fraction of tumors respond to radiotherapy (responders) while the majority are nonresponsive. We determined that responding tumors had increased damage-induced cell death, and a unique immune-activation signature associated with tumor-associated macrophages, cancer-associated fibroblasts, and CD8+ T cells. This signature was dependent on radiation-induced increases of Type I interferons (IFNs). We investigated a therapeutic approach targeting the cGAS/STING pathway and demonstrated improved response rate following radiotherapy. These results suggest that modulating the Type I IFN pathway has the potential to improve radiation therapy efficacy in RC.
Demonstration of cross reaction in hybrid graphene oxide/tantalum dioxide guided mode resonance sensor for selective volatile organic compound
Authors: TANTIWANICHAPAN KHWANCHAI , JOLIVOT ROMUALD , JOMPHOAK APICHAI , SRIS NANTARAT , CHANANONNAWATHORN CHANUNTHORN , LERTVANITHPOL TOSSAPORN , HORPRATHUM MATI , BOONRUANG SAKOOLKAN
Published: April 2023

This paper experimentally demonstrates a crossed reaction of pure and hybrid graphene oxide (GO)/tantalum dioxide (TaO2) as a volatile organic compound (VOC) absorber in a guided mode resonance (GMR) sensing platform. The proposed GMR platform has a porous TaO2 film as the main guiding layer, allowing for more molecular adsorption and enhanced sensitivity. GO is applied on top as an additional VOC absorber to increase the selectivity. The hybrid sensing mechanism is introduced by varying the concentration of the GO aqueous solution. The experimental results show that the pure TaO2-GMR has a high tendency to adsorb most of the tested VOC molecules, with the resonance wavelength shifting accordingly to the physical properties of the VOCs (molecular weight, vapor pressure, etc.). The largest signal appears in the large molecule such as toluene, and its sensitivity is gradually reduced in the hybrid sensors. At the optimum GO concentration of 3 mg/mL, the hybrid GO/TaO2 -GMR is more sensitive to methanol, while the pure GO sensor coated with GO at 5 mg/mL is highly selective to ammonia. The sensing mechanisms are verified using the distribution function theory (DFT) to simulate the molecular absorption, along with the measured functional groups measured on the sensor surface by the Fourier transform infrared spectroscopy (FTIR). The crossed reaction of these sensors is further analyzed by means of machine learning, specifically the principal component analysis (PCA) method and decision tree algorithm. The results show that this sensor is a promising candidate for quantitative and qualitative VOCs detection in sensor array platform.
Volatile chemical constituents of two species of bryophytes (Bryophyta) occurring in the Brazilian Amazon
Authors: Rezende-Moraes Eryka de Nazaré , Santos Rita de Cássia Pereira dos , Miranda Thyago Gonçalves , Alves Raynon Joel Monteiro , Andrade Eloisa Helena De Aguiar , Zoghbi Maria Das Graças Bichara , Costa Roberto Cezar Lobo da , Tavares-Martins Ana Cláudia Caldeira
Published: April 2023

Bryophytes are a group of plants that present a variety of chemical compounds, but information on the phytochemistry of the species, especially those in the Brazilian Amazon, is still scant. Thus, the objective of this work was to determine the volatile chemical constituents of the Amazonian moss species Sematophyllum subsimplex (Hedw). Mitt. and Leucobryum martianum (Hornsch.) Hampe ex Mull Hal. Samples were collected at the Gunma Ecological Park, in the municipality of Santa Bárbara, Pará, and subsequently dried, identified, screened, kept in a refrigerated place, and subjected to extraction by means of simultaneous distillation-extraction (SDE). Volatile concentrates were analyzed by gas chromatography/mass spectrometry (GC-MS). Forty-two chemical constituents were identified in the pentane extracts of the two species studied, mainly belonging to the group of aldehydes, terpenes, and carboxylic acids. Naphthalene was predominant in both species, mainly in L. martianum. Safrole, found in L. martianum, is being recorded for the first time in a moss. This study represents the first report of the chemical composition of the two selected taxa and the results indicate that these mosses are natural sources of compounds of interest for bioprospecting.
Chimeric antigen receptor T cells targeting cell surface GRP78 efficiently kill glioblastoma and cancer stem cells
Authors: WANG SHIJIE , Wei Wenwen , Yuan Yuncang , Sun Bin , Yang Dong , Liu Nan , Zhao Xudong
Published: April 2023

Background: Glioblastoma (GBM) is recognized as among the most aggressive forms of brain tumor. Patients typically present with a five-year survival rate of less than 6% with traditional surgery and chemoradiotherapy, which calls for novel immunotherapies like chimeric antigen receptor T (CAR-T) cells therapy. In response to endoplasmic reticulum (ER) stress in multiple tumor cells including GBM, the glucose-regulated protein 78 (GRP78) expression increases and the protein is partially translocated to the cell surface, while it is restricted to the cytoplasm and the nucleus in normal cells.
Methods: In this study, to target the cell surface GRP78 (csGRP78), CAR-T cells based on its binding peptide were generated. In vitrotwo GBM cell lines and glioma stem cells (GSCs) were used to confirm the localization of csGRP78 and the cytotoxicity of the CAR-T cells. In vivoa GBM xenograft model was used to assess the killing activity and the safety of the CAR-T cells.
Results: We confirmed the localization of csGRP78 at the cell surface of two GBM cell lines (U-251MG and U-87MG) and in GSCs. Co-culture experiments revealed that the CAR-T cells could specifically kill the GBM tumor cells and GSCs with specific IFN-γ release. Furthermore, in the tumor xenograft model, the CAR-T cells could decrease the number of GSCs and significantly suppress tumor cell growth. Importantly, we found no obvious off-target effects or T cell infiltration in major organs following systemic administration of these cells.
Conclusions: The csGRP78 targeted CAR-T cells efficiently kill GBM tumor cells and GSCs both in vitro and in vivo, and ultimately suppress the xenograft tumors growth without obvious tissue injuries. Therefore, our study demonstrates that csGRP78 represents a valuable target and the csGRP78-targeted CAR-T cells strategy is an effective immunotherapy against GBM.
Genome-wide analysis and expression profiling of Aspartyl tRNA-synthetase (AspRS) family genes reveal function of drought stress response in poplar
Authors: Feng Cong-Hua , Niu Meng-Xue , Zhao Shilei , Guo Shangjing , Yin Weilun , Xia Xinli , Su Yanyan
Published: April 2023

Background: Drought stress is a prevalent abiotic stress that significantly hinders the growth and development of plants. According to studies, β-aminobutyric acid (BABA) can influence the ABA pathway through the AtIBI1 receptor gene to enhance cold resistance inArabidopsis. However, the Aspartate tRNA-synthetase (AspRS) gene family, which acts as the receptor for BABA, has not yet been investigated in poplar. Particularly, it is uncertain how the AspRS gene family (PtrIBIs)r can resist drought stress after administering various concentrations of BABA to poplar.
Results: In this study, we have identified 12 AspRS family genes and noted that poplar acquired four PtrIBI pairs through whole genome duplication (WGD). We conducted cis-action element analysis and found a significant number of stress-related action elements on different PtrIBI genes promoters. The expression of most PtrIBI genes was up-regulated under beetle and mechanical damage stresses, indicating their potential role in responding to leaf damage stress. Our results suggest that a 50 mM BABA treatment can alleviate the damage caused by drought stress in plants. Additionally, via transcriptome sequencing, we observed that the partial up-regulation of BABA receptor genes, PtrIBI2/4/6/8/11, in poplars after drought treatment. We hypothesize that poplar responds to drought stress through the BABA-PtrIBIs-PtrVOZ coordinated ABA signaling pathway. Our research provides molecular evidence for understanding how plants respond to drought stress through external application of BABA.
Conclusions: In summary, our study conducted genome-wide analysis of the AspRS family of P. trichocarpa and identified 12 PtrIBI genes. We utilized genomics and bioinformatics to determine various characteristics of PtrIBIs such as chromosomal localization, evolutionary tree, gene structure, gene doubling, promoter cis-elements, and expression profiles. Our study found that certain PtrIBIgenes are regulated by drought, beetle, and mechanical damage implying their crucial role in enhancing poplar stress tolerance. Additionally, we observed that external application of low concentrations of BABA increased plant drought resistance under drought stress. Through the BABA-PtrIBIs-PtrVOZsignaling module, poplar plants were able to transduce ABA signaling and regulate their response to drought stress. These results suggest that the PtrIBIgenes in poplar have the potential to improve drought tolerance in plants through the topical application of low concentrations of BABA.
Surveillance of the Field-Incurred Pesticide Residues in Kinnow Mandarin and Evaluation of Acute, Chronic and Cumulative Exposure Risks to the Consumers
Authors: BIBI AASMA , RAFIQ NAZIA , KHALID SOFIA , AHAD KARAM , SAMAD ABDUL , MEHBOOB FARRAKH
Published: April 2023

Citrus fruits may serve as potential dietary exposure pathways to toxic levels of pesticides, with which they are treated. For possible protection of the consumers, pesticide residues in citrus fruits need strict and regular monitoring. This study aimed at investigating the pesticide residues status of the raw/fresh citrus fruits i.e. Kinnow Mandarin, along with evaluating exposure risks from the detected pesticides through Kinnow Mandarin consumption. Overall, 22.5% of the samples were non-complaint to the CAC-MRLs (Codex Alimentarius Commission’s established maximum residue limits) and 77.5% were exceeding the MRLs set by EU (European Union). Bifenthrin, with highest detection rate (53%), is a registered pyrethroid acaricide/insecticide for use on citrus fruits and is among the potential endocrine disrupting substances. The estimated acute/short-term (%aRfD) and chronic/long-term (%ADI) exposure values were in the range of 0.12–29.12% and 0.001–1.186%, respectively, not exceeding the hazard limit of 100% in any case. The cumulative toxicity evaluation performed for the detected neurotoxic pesticides belonging to pyrethroid, organophosphate and neonicotinoid group, indicated low potential long-term health risks from citrus fruits consumption. The results of the health risks’ estimations denote citrus fruits consumption a safer option for its consumers.
A Rare Case of Aggressive Histiocytic Sarcoma in a 9- years old male Child: Case report and Literature review
Authors: Arega Gashaw , Hailu Daniel , Nigusie Mulualeme , Fikad Getasew , Getachew Hanna , Kiflu Woubedel , Semere Yodit , Sisay Samuel , Zeray Abrehet , Asefa Mesfin , Dagmawi Fitsum , Tesfaye Kalkidan , Tadesse Abel , M.Seid Abdulkadir
Published: April 2023

Histiocytic Sarcoma(HS) is an extremely rare non-Langerhans neoplasm primarily composed of malignant cells with morphologically and immunohistochemically showing features of mature tissue histiocytes. It can affect any part of the body with unknown etiology and is an aggressive disease with a worse prognosis and management often requires multimodal treatment. Here, we report a 9 year old boy who was diagnosed with histiocytic sarcoma after he presented with left-side chest pain, dry cough, and significant weight loss of four months duration. Contrast-enhanced chest CT scan shows a heterogeneous contrast-enhancing solid extra parenchymal mass lesion that appears to be attached to the posteromedial basal pleura with adjacent lung subsegmental collapse and a minimal amount of ipsilateral pleural effusion. There was no evidence of systemic arterial supply. The patient underwent left posterolateral thoracotomy and mass excision. The biopsy and immunohistochemical examination confirmed the diagnosis of Histiocytic Sarcoma. The mass recurred after a month, and he started systemic chemotherapy with a CHOP regimen. Despite the multimodal treatment, he had a progressive disease with multiorgan involvement and was put on palliative care.
DeepASDPred: A CNN-LSTM-based deep learning method for Autism spectrum disorders risk RNA identification
Authors: Fan Yongxian , Xiong Hui , Sun Guicong
Published: April 2023

Background Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders characterized by difficulty communicating with society and others, behavioral difficulties, and a brain that processes information differently than normal. Genetics has a strong impact on ASD associated with early onset and distinctive signs. Currently, all known ASD risk genes are able to encode proteins, and some de novo mutations disrupting protein-coding genes have been demonstrated to cause ASD. Next-generation sequencing technology enables high-throughput identification of ASD risk RNAs. However, these efforts are time-consuming and expensive, so an efficient computational model for ASD risk gene prediction is necessary.Results In this study, we propose DeepASDPerd, a predictor for ASD risk RNA based on deep learning. Firstly, we use K-mer to feature encode the RNA transcript sequences, and then fuse them with corresponding gene expression values to construct a feature matrix. After combining chi-square test and logistic regression to select the best feature subset, we input them into a binary classification prediction model constructed by convolutional neural network and long short-term memory for training and classification. The results of the 10-fold cross-validation proved our method outperformed the state-of-the-art methods. Dataset and source code are available at https://github.com/Onebear-X/DeepASDPred is freely available.Conclusions Our experimental results show that DeepASDPred has outstanding performance in identifying ASD risk RNA genes.
Depression Detection on Social Media: A Classification Framework and Research Challenges and Opportunities
Authors: Aldkheel Abdulrahman , Zhou Lina
Published: April 2023

Objective: Social media has become a safe space for discussing sensitive topics such as mental disorders. Depression dominates mental disorders globally, and accordingly, depression detection on social media has witnessed significant research advances. This study aims to review the current state-of-the-art research methods and propose a multidimensional framework to describe the current body of literature relating to detecting depression on social media.
Method: A study methodology involved selecting papers published between 2011 and 2022 that focused on detecting depression on social media. Three digital libraries were used to find relevant papers: Google Scholar, ACM digital library, and ResearchGate. In selecting literature, two fundamental elements were considered: identifying papers focusing on depression detection and including papers involving social media use.
Results: In total, 46 papers were reviewed. Multiple dimensions were analyzed, including input features, social media platforms, disorder and symptomatology, ground truth, and machine learning. Various types of input features were employed for depression detection, including textual, visual, behavioral, temporal, demographic, and spatial features. Among them, visual and spatial features have not been systematically reviewed to support mental health researchers in depression detection. Despite depression's fine-grained disorders, most studies focus on general depression.
Conclusion: Recent studies have shown that social media data can be leveraged to identify depressive symptoms. Nevertheless, further research is needed to address issues like depression validation, generalizability, causes identification, and privacy and ethical considerations. An interdisciplinary collaboration between mental health professionals and computer scientists may help detect depression on social media more effectively.
Electrochemical determination of tert-butylhydroquinone by ZIF- 67@TiO 2 derived hierarchical TiO 2 /Co/NCNTs
Authors: Zheng Shengbiao , Fan Jialong , Yin Feilong , Chen Jie , Hui Zhenzhen , Tang Jing , Wang Xuchun , Guo Jiahao
Published: April 2023

Heterostructured TiO2/Co nanoparticles/N-doped carbon nanotubes (TiO2/Co/NCNTs) nanomaterial had been derived from ZIF-67@TiO2 by calcination at high temperature in an Ar/H2 atmosphere. The obtained TiO2/Co/NCNTs composites was made up of numerous dodecahedron-like boxes with twisting nanotubes on surfaces, modified on glassy carbon electrode (GCE) for the electrochemical determination of tert-butylhydroquinone (TBHQ). The excellent electrocatalytic performance for TBHQ could be owing to the combination of multi-phase composition, fast electron transfer and the constructed Co/Ti multi-active sites. TiO2/Co/NCNTs sensor held broad linearity from 0.04 to 100 µM with a low detection limit of 4 nM, and possessed stability, anti-interfering ability and reproducibility. Finally, the analytical utility of the TiO2/Co/NCNTs sensor was checked by detecting TBHQ in the edible oil.
Multidimensional functionality limit states for seismic resilience analysis of urban buildings
Authors: Gutiérrez Juan , Ayala Amado Gustavo , López Saúl Esteban
Published: April 2023

Currently, most of methodologies available to evaluate the seismic performance of buildings use as index maximum interstory drifts. However, recent earthquakes have evidenced the need to develop performance levels that incorporate seismic resilience concepts to evaluate the level of post-seismic functionality of buildings and their capacity to recover functionality. Furthermore, such performance levels should explicitly consider the performance of structural, non-structural elements and contents. For this purpose, this paper proposes a set of six performance limit states for office-type buildings, in which the seismic performance of structural, non-structural elements and contents is explicitly considered. Each of these limit states is associated with a set of probable events that generically determine its recovery of functionality (e.g., post-seismic inspection and management of financial resources). To exemplify the proposed scheme a seven-story reinforced concrete building with unreinforced infill masonry walls and located in Mexico City is evaluated. The results obtained suggest that the building has a significant probability of experiencing loss of functionality due to the damage suffered mainly by the non-structural elements and contents. This indicates that modern seismic design codes, as that used for this study, accomplish their main objective, which is to reduce the probability of collapse and to prevent the loss of human lives. However, these results also demonstrate that the main objective of decision makers when designing such buildings, which is to be functional for one or several needs, is not achieved.
Modeling the Effect of Sustainable Land Management Program Intervention Work on land Use Land Cover Change in Amba 1 and Temba watershed, Benishangul Gumuz Region, Ethiopia
Authors: Shitu Kasye , Mekuriaw Mastewal , Degu Tigist , Hymiro Adibar , Biratu Tadesse , Misgana Workiye
Published: April 2023

Benishangul Gumuz Region in Ethiopia has been experiencing rapid land use and land cover change through illegal deforestation for shifting agriculture, livestock production, and fuel consumption. However, a sustainable land management program (SLMP) may be the way to curb this rapid land use land cover change in the micro watershed level in the Region. On the basis of this, to assess, the benefits after a 12-year micro watershed restoration effort, this research was conducted in two micro watersheds to investigate the land use land cover dynamics using intensive field observation, interview, and time series Landsat ( ETM + and OLI_TIRS) satellite data since 2010 up to 2021. After classifying the land use of the two micro watersheds (Amba1 and Temba) as Forest Lands, Bush Lands, Settlements, Grass Lands, Crop Lands, and Bare Lands for the years 2010, 2015, and 2021 the calculation of land use change dynamics result indicates significant incensement for forest land and settlements area from the year 2010 up to 2021. Whereas, the area of Bare Lands indicates a significant decrement up to 82.234% in Amba1 micro watershed. This result is an indicator that, sustainable land management program (SLMP) intervention can greatly improve land cover change for batter use in Ethiopia.
Nonlinear absorption features of organic dye in polar solvents: Role of solvents on solute molecule
Authors: S Jeyaram
Published: April 2023

This paper focuses the study of nonlinear absorption (NLA) features of anthraquinone dye namely, acid blue 129 with various polar solvents including ethanol, methanol, acetone, 1-proponal and DMSO. The acid blue 129 dye in polar solvents is characterized by UV-Visible absorption and emission study. The NLA measurements of acid blue 129 dye is examined via open aperture (OA) Z-scan method employing at 650 nm wavelength. The OA curve of the experimental dye exhibits both saturable absorption (SA) and reveres saturable absorption (RSA) characters, which results from negative and positive NLA coefficient (β). The order of ground and excited state absorption cross-section is measured to be 10˗22 m2 and 10˗18 m2, respectively and proved the existence RSA in acid blue 129 dye. The order of NLA coefficient and imaginary component of the third-order nonlinear susceptibility of acid blue 129 dye in polar solvent is calculated to be 10˗3 cm/W and 10˗5 esu, respectively. The multi-parameter scale viz., Kamlet-Abboud-Taft is used to measure the influence of solvent parameters on solute molecule. The results from the experiments suggest that the acid blue 129 is a suitable candidate for nonlinear optical (NLO) applications.
An Improved Channel Flow Measurement Approach Using Conical Central Baffle Flumes
Authors: Nair P Sujith , Ghare Aniruddha. D. , Kapoor Ankur
Published: April 2023

A simple and inexpensive way of channel discharge measurement is to introduce an axial obstruction in the flow cross- section, thereby creating the critical flow conditions. Such flow measuring devices are referred to as baffle flumes. Literature showed that the critical flow conditions are routinely assumed to occur at the minimum flow cross-section in the flume, however practically the critical section lies slightly on the upstream side. This paper presents an analytical approach to deduce the discharge prediction model on the basis of identification of true location of critical flow section that lies near the vicinity of the least constricted section in the baffle flume. Using the experimental data, a relationship between the ratio of energy to critical depth and the geometrical configuration of the baffle flume having conical shaped obstruction in a trapezoidal channel section, is obtained. Coupled with the expression for critical contracted width, the location of the critical flow section is established. Numerical analysis has also been carried out to ascertain the applicability of the proposed discharge prediction model. The discharge prediction model presented herein for the Conical Central Baffle Flume, is found to provide the discharge with better accuracy (maximum error 2.49%) when compared with the Kapoor et al. (2021) model available in the literature (maximum error 5.24%). An illustrative example to demonstrate use of the proposed methodology, is also appended herein for the benefit of the field hydraulic engineers dealing with flow measurements.
Chemical Characterization of Indian Smokeless tobacco products by spectroscopy, chromatography, and allied techniques: Interproduct Variation
Authors: Jain Nilesh , Jhamtani Reena C. , Gupta Shilpa , Varman SV Rajith
Published: April 2023

Background: This investigation provides an updated study measuring the levels of chemical components for the top-selling smokeless tobacco products (SLT) viz. khaini, mishri, pan masala + tobacco, snus, and tobacco-betel nut mixture in the Indian market.
Methods:A total of twelve SLT were collected and analyzed for nitrosamines and polycyclic aromatic hydrocarbons using various standard techniques of gas and liquid chromatography(as GC-MS, GC-TEA, LC-MS/MS)at two different laboratories and compared to previous studies.
Results: Mishri contains a major share of tobacco-specific nitrosaminesas NNN (10 folds where folds refer to a comparison of average) and NNK (25-30 folds) with higher ratio than other SLT. Total polycyclic aromatic hydrocarbons content of mishri quantified to be 702-794 µg/g of SLT which was 7-9 fold higher than snus. On geographical distribution, SLT composition reflects inter and intra-differences. TSNA levels were drastically different between mishri and khaini samples. This extensive variation is attributed to tobacco curing and other manufacturing techniques.
Conclusions: Overall, findings indicate mishri to be most harmful among all SLT whereas snus can be considered as an alternative for better public health.The chemical characterization provides a broader understanding of differences in carcinogenic-potential relating to the concentration-variation between the products. Also, the present study would create a database to ensure the consumer's right to be informed about health-risk from a product and make aware of the need to monitor and regulate the ingredients among all SLT used in the country.
Primary osteolytic pediatric extradural complicated hydatid cyst revealed by a skull vault swelling: a case report and literature review.
Authors: Borni Mehdi , Abdelmouleh Souhir , Tallah Marouen , Blibeche Hela , Ayadi Ali , Boudawara Mohamed Zaher
Published: April 2023

Hydatidosis is a parasitic infection whose etiological agent is the larva of the cestode Echinococcus granulosus. It is a zoonosis and the human being behaves as an accidental intermediate host in the parasitic cycle. The most frequent clinical presentation is hepatic, followed by pulmonary, with cerebral hydatidosis being extremely rare. Imaging is characteristic, generally dealing with single cystic lesion, usually unilocular and less frequently multilocular, located mainly intraaxially. The primary disease remains extremely rare and its clinical picture is related to the number, size, and location of the lesions. Infection within these cerebral hydatid cysts remains an extremely rare occurrence and only few cases reported previously in the literature. The authors report the nosological review of the clinical, imaging, surgical, and histopathological records of an uncommon primary osteolytic pediatric extradural complicated hydatid cyst in a 5 year-old North African male patient coming from a rural area who presented for progressive onset of a painless left parieto-occipital soft swelling without any neurological disorder with good outcomes after surgery. The authors report this case due the fact that it had not been documented before in the pediatric population and to the success of the specialized treatment.
Research on PV mode diffusion considering the game among enterprises in the complex network context
Authors: Li Feng , zhang Pengchao , Chen Wenting , Li Huimin , Fei Xiaoxia
Published: April 2023

With the emergence of energy shortages and ecological deterioration in China, the deployment of distributed photovoltaic (PV) as a renewable energy source in rural areas has been recognized as a potential solution for reducing greenhouse gas emissions. This research aims at at investigating the selection of promotion modes for PV installation enterprises in China by utilizing complex network theory to construct a model. The proposed model analyzes the impact of various factors, including villagers' support rate, the vacillation rate, and PV operation and maintenance costs, within an evolutionary game theory on the selection of promotion modes for enterprises. The findings of this study indicate that the villagers' support rate has the most significant influence on the promotion modes selected by enterprises. Therefore, it is crucial to establish appropriate incentives, increase publicity efforts, and educate the villagers about the benefits of PV installation for themselves and the environment. Additionally, PV installation enterprises must prioritize equipment operation and maintenance to ensure the efficient and stable operation of PV devices, which is critical for achieving the energy conservation, the emission reduction, and stable income. Ultimately, this research provides valuable insights for PV installation enterprises to promote distributed PV in rural areas by adjusting the parameter settings based on the evolution results. By adopting the proposed model, PV installation enterprises can optimize their promotion strategies and enhance their chances of success in the rural market, thereby contributing to the reduction of greenhouse gas emissions and achieving sustainable development goals.
Efficacy of adjuvant radiation in early stage cancers of buccal mucosa- an Institutional experience
Authors: Goyal Surekha , Ikkurthi Vedamanasa , N Geeta S
Published: April 2023

Purpose
The purpose of this study is to determine if depth of infiltration is the only risk factor that will determine the outcomes in early stage buccal mucosa patients or do the other minor risk factors have an impact too.
Methods
It is a retrospective analysis of 226 patients with early stage buccal mucosa cancer who were treated with curative intent from 2010 to 2020. These patients were grouped in two arms, surgery alone(n=111) and surgery followed by adjuvant radiotherapy( n=115).
Patients were followed up and local and regional recurrences and distant metastasis were recorded.
Results
Our results show that addition of Radiation to the standard surgery arm improves overall survival and disease free survival, though the improvement in overall survival was not statistically significant. This improvement was more pronounced in the depth of infiltration >5mm and in 5mm or less depth of infiltration this benefit was not statistically significant. Other factors like perineural invasion, lymphovascular invasion, tumour size, node positive, margin positive were considered for univariate analysis. Although there was a trend towards improvement of OS and DFS it was not statistically significant as far as these factors are concerned.
Conclusion
The role of adjuvant radiation in early stage cancers of buccal mucosa is a crucial tool with definitive DFS benefit and requires more prospective trials to answer the OS benefit.
First report of tomato zonate spot virus on green pepper in Japan
Authors: Shimada Ryoko , Okamoto Masahiro , Tomitaka Yasuhiro
Published: April 2023

In 2021, necrotic spot of leaves and stem necrosis were observed on green pepper (Capsicum annuum L.) in Kanagawa Prefecture, Japan. The virus isolated through two-rounds of single lesion isolation, the nucleotide sequence of the N gene of the isolate shared 95%identity with that of the tomato zonate spot virus (TZSV) reported in China. When the isolated virus was inoculated to green pepper plants, the original symptoms appeared. In addition, TZSV was detected from the inoculated plants by RT-PCR. This is the first report of occurrence of TZSV in Japan.
Preparation of CeO 2 -PPy nanocomposites to improve the corrosion resistance of epoxy resin coatings
Authors: Feng Shangce , Ma Li , Liu Mengli , Tian Luming , He Deliang
Published: April 2023

Here, CeO2 was used as the sole oxidant for pyrrole polymerization via in-situ chemical oxidation, and polypyrrole (PPy) was grown on the CeO2 surface to form CeO2-PPy nanocomposites. The morphology of CeO2-PPy nanocomposites was observed using SEM. The electrochemical corrosion behavior of the coatings in 3.5 wt% NaCl solution was investigated through electrochemical impedance spectroscopy (EIS) measurements. After 40 days of immersion, the coating with CeO2-PPy nanocomposite fillers exhibited outstanding anti-corrosion performance, and the |Z|0.01 Hz value exceeded 108 Ω·cm2, which was one order of magnitude higher than of the pure epoxy coating. The enhanced corrosion protection was attributed to the complex passive film produced by the synergistic effect of PPy and cerium(Ⅲ) ions. The results showed that CeO2-PPy nanocomposites can be used as fillers for the corrosion protection of steel substrates.