bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Table S1 from Genomic Profiling of Metastatic Castration-Resistant Prostate Cancer Samples Resistant to Androgen Receptor Pathway Inhibitors
Authors: Menssouri Naoual , Poiraudeau Loïc , Helissey Carole , Bigot Ludovic , Sabio Jonathan , Ibrahim Tony , Pobel Cédric , Nicotra Claudio , Ngo-Camus Maud , Lacroix Ludovic , Rouleau Etienne , Tselikas Lambros , Chauchereau Anne , Blanc-Durand Félix , Bernard-Tessier Alice , Patrikidou Anna , Naoun Natacha , Flippot Ronan , Colomba Emeline , Fuerea Alina , Albiges Laurence , Lavaud Pernelle , van de Wiel Paul , den Biezen Eveline , Wesseling-Rozendaal Yvonne , Ponce Santiago , Michiels Stefan , Massard Christophe , Gautheret Daniel , Barlesi Fabrice , André Fabrice , Besse Benjamin , Scoazec Jean-Yves , Friboulet Luc , Fizazi Karim , Loriot Yohann
Published: July 2023

The Supplementary table "Representativeness of Study Participants" reports several comments regarding the representativeness of study participants including age, geography and overall representativeness of the study.
Supplementary Methods S1 from Genomic Profiling of Metastatic Castration-Resistant Prostate Cancer Samples Resistant to Androgen Receptor Pathway Inhibitors
Authors: Menssouri Naoual , Poiraudeau Loïc , Helissey Carole , Bigot Ludovic , Sabio Jonathan , Ibrahim Tony , Pobel Cédric , Nicotra Claudio , Ngo-Camus Maud , Lacroix Ludovic , Rouleau Etienne , Tselikas Lambros , Chauchereau Anne , Blanc-Durand Félix , Bernard-Tessier Alice , Patrikidou Anna , Naoun Natacha , Flippot Ronan , Colomba Emeline , Fuerea Alina , Albiges Laurence , Lavaud Pernelle , van de Wiel Paul , den Biezen Eveline , Wesseling-Rozendaal Yvonne , Ponce Santiago , Michiels Stefan , Massard Christophe , Gautheret Daniel , Barlesi Fabrice , André Fabrice , Besse Benjamin , Scoazec Jean-Yves , Friboulet Luc , Fizazi Karim , Loriot Yohann
Published: July 2023

supplementary methods
Supplementary Data S12 from Genomic Profiling of Metastatic Castration-Resistant Prostate Cancer Samples Resistant to Androgen Receptor Pathway Inhibitors
Authors: Menssouri Naoual , Poiraudeau Loïc , Helissey Carole , Bigot Ludovic , Sabio Jonathan , Ibrahim Tony , Pobel Cédric , Nicotra Claudio , Ngo-Camus Maud , Lacroix Ludovic , Rouleau Etienne , Tselikas Lambros , Chauchereau Anne , Blanc-Durand Félix , Bernard-Tessier Alice , Patrikidou Anna , Naoun Natacha , Flippot Ronan , Colomba Emeline , Fuerea Alina , Albiges Laurence , Lavaud Pernelle , van de Wiel Paul , den Biezen Eveline , Wesseling-Rozendaal Yvonne , Ponce Santiago , Michiels Stefan , Massard Christophe , Gautheret Daniel , Barlesi Fabrice , André Fabrice , Besse Benjamin , Scoazec Jean-Yves , Friboulet Luc , Fizazi Karim , Loriot Yohann
Published: July 2023

Supplementary data 12 describes the GSEA analysis of resistant samples vs baseline samples from patients with acquired resistance
Supplemental Figure 5 from Mitochondrial Uncoupling Inhibits Reductive Carboxylation in Cancer Cells
Authors: Jiang Haowen , He Clifford Jiajun , Li Albert M. , He Bo , Li Yang , Zhou Meng-Ning , Ye Jiangbin
Published: July 2023

S5. Mitochondrial uncoupler NEN, FCCP and BAM15 inhibit reductive carboxylation under hypoxia.
Supplementary Table 1 from LncRNA-Encoded Micropeptide ACLY-BP Drives Lipid Deposition and Cell Proliferation in Clear Cell Renal Cell Carcinoma via Maintenance of ACLY Acetylation
Authors: Zhang Shenghua , Zhang Zheng , Liu Xiaolong , Deng Yibin , Zheng Jian , Deng Jieqiong , Wang Yirong , Guo Binbin , Li Fanrong , Chen Xiaoyue , Pan Yacheng , Wang Jieyu , Lu Jiachun , Wu Siqi , Guo Qiang , Zhou Yifeng
Published: July 2023

Primary clinical and histological characteristics of 67 study patients
Supplementary Figure 4 from LncRNA-Encoded Micropeptide ACLY-BP Drives Lipid Deposition and Cell Proliferation in Clear Cell Renal Cell Carcinoma via Maintenance of ACLY Acetylation
Authors: Zhang Shenghua , Zhang Zheng , Liu Xiaolong , Deng Yibin , Zheng Jian , Deng Jieqiong , Wang Yirong , Guo Binbin , Li Fanrong , Chen Xiaoyue , Pan Yacheng , Wang Jieyu , Lu Jiachun , Wu Siqi , Guo Qiang , Zhou Yifeng
Published: July 2023

Related information to figure 6
Data from Mitochondrial Uncoupling Inhibits Reductive Carboxylation in Cancer Cells
Authors: Jiang Haowen , He Clifford Jiajun , Li Albert M. , He Bo , Li Yang , Zhou Meng-Ning , Ye Jiangbin
Published: July 2023

Abstract
When the electron transport chain (ETC) function is impaired, cancer cells rely on reductive carboxylation (RC) to convert α-ketoglutarate (αKG) to citrate for macromolecular synthesis, thereby promoting tumor growth. Currently, there is no viable therapy to inhibit RC for cancer treatment. In this study, we demonstrate that the mitochondrial uncoupler treatment effectively inhibits RC in cancer cells. Mitochondrial uncoupler treatment activates the ETC and increases the NAD+/NADH ratio. Using U–13C-glutamine and 1–13C-glutamine tracers, we show that mitochondrial uncoupling accelerates the oxidative tricarboxylic acid (TCA) cycle and blocks RC under hypoxia, in von Hippel-Lindau (VHL) tumor suppressor–deficient kidney cancer cells, or under anchorage-independent growth condition. Together, these data demonstrate that mitochondrial uncoupling redirects α-KG from RC back to the oxidative TCA cycle, highlighting that the NAD+/NADH ratio is one key switch that determines the metabolic fate of α-KG. Inhibiting RC could be a key mechanism by which mitochondrial uncouplers inhibit tumor growth.
Implications:
Mitochondrial uncoupling is a novel strategy to target RC in cancer.
Data from Allele-specific Gene Regulation, Phenotypes, and Therapeutic Vulnerabilities in Estrogen Receptor Alpha–mutant Endometrial Cancer
Authors: Blanchard Zannel , Rush Craig M. , Arnesen Spencer , Vahrenkamp Jeffery M. , Rodriguez Adriana C. , Jarboe Elke A. , Brown Callie , Chang Matthew E.K. , Flory Mark R. , Mohammed Hisham , Modzelewska Katarzyna , Lum David H. , Gertz Jason
Published: July 2023

Abstract
Activating estrogen receptor alpha (ER; also known as ESR1) mutations are present in primary endometrial and metastatic breast cancers, promoting estrogen-independent activation of the receptor. Functional characterizations in breast cancer have established unique molecular and phenotypic consequences of the receptor, yet the impact of ER mutations in endometrial cancer has not been fully explored. In this study, we used CRISPR-Cas9 to model the clinically prevalent ER-Y537S mutation and compared results with ER-D538G to discover allele-specific differences between ER mutations in endometrial cancer. We found that constitutive activity of mutant ER resulted in changes in the expression of thousands of genes, stemming from combined alterations to ER binding and chromatin accessibility. The unique gene expression programs resulted in ER-mutant cells developing increased cancer-associated phenotypes, including migration, invasion, anchorage-independent growth, and growth in vivo. To uncover potential treatment strategies, we identified ER-associated proteins via Rapid Immunoprecipitation and Mass Spectrometry of Endogenous Proteins and interrogated two candidates, CDK9 and NCOA3. Inhibition of these regulatory proteins resulted in decreased growth and migration, representing potential novel treatment strategies for ER-mutant endometrial cancer.
Implications:
This study provides insight into mutant ER activity in endometrial cancer and identifies potential therapies for women with ER-mutant endometrial cancer.
Supplementary Figure 8 from Activating Inducible T cell Co-stimulator (ICOS) yields anti-tumor activity alone and in combination with anti-PD-1 checkpoint blockade
Authors: Yadavilli Sapna , Waight Jeremy D , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J , Hopson Christopher B , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: July 2023

Pharmacokinetic analysis of the mIgG1 anti-ICOS antibody
(7E.17G9) in the peripheral blood of EMT6 tumor-bearing mice.
Supplementary Figure 3 from Activating Inducible T cell Co-stimulator (ICOS) yields anti-tumor activity alone and in combination with anti-PD-1 checkpoint blockade
Authors: Yadavilli Sapna , Waight Jeremy D , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J , Hopson Christopher B , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: July 2023

Affinity and kinetics
Supplementary Figure S13 from LNS8801 inhibits Acute Myeloid Leukemia by inducing the production of reactive oxygen species and activating the endoplasmic reticulum stress pathway
Authors: Lee Inyoung , Doepner Miriam , Weissenrieder Jillian S , Majer Ariana D , Mercado Sophia , Estell Angela , Natale Christopher A , Sung Pamela J , Foskett J. Kevin , Carroll Martin , Ridky Todd W
Published: July 2023

Supplementary Figure S13. IRE1⍺ inhibitor blocks LNS8801-induced caspase cleavage Western blot of U937 cells treated with 250nM LNS8801 and/or 1uM IRE1⍺ inhibitor Kira6 or 5uM PERK inhibitor GSK414 for 24 hours. Western blot quantification was done by normalizing each band to the respective actin band.
Supplementary Figure S12 from LNS8801 inhibits Acute Myeloid Leukemia by inducing the production of reactive oxygen species and activating the endoplasmic reticulum stress pathway
Authors: Lee Inyoung , Doepner Miriam , Weissenrieder Jillian S , Majer Ariana D , Mercado Sophia , Estell Angela , Natale Christopher A , Sung Pamela J , Foskett J. Kevin , Carroll Martin , Ridky Todd W
Published: July 2023

(A and B) Western blots of (A) U937 and (B) MOLM14 cells treated with 25nM estradiol (E2) or 50nM thapsigargin (Tg) for indicated time. Western blot quantification was done by normalizing each band to its respective actin band.
Supplementary Figure 1 from Activating Inducible T cell Co-stimulator (ICOS) yields anti-tumor activity alone and in combination with anti-PD-1 checkpoint blockade
Authors: Yadavilli Sapna , Waight Jeremy D , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J , Hopson Christopher B , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: July 2023

Exemplar flow cytometry gating strategy.
Supplementary Figure 8 from Metronomic Administration of Topotecan Alone and in Combination with Docetaxel Inhibits Epithelial–mesenchymal Transition in Aggressive Variant Prostate Cancers
Authors: Mitra Ghosh Taraswi , Mazumder Suman , Davis Joshua , Yadav Jyoti , Akinpelu Ayuba , Alnaim Ahmed , Kumar Harish , Waliagha Razan , Church Bird Allison E. , Rais-Bahrami Soroush , Bird R. Curtis , Mistriotis Panagiotis , Mishra Amarjit , Yates Clayton C. , Mitra Amit K. , Arnold Robert D.
Published: July 2023

Supplementary Fig. 8 shows Flow Cytometry; Prostate cancer cell lines stained with stemness markers (CD44, CD133 and CD44/133) to determine the percent of CD44+, CD133+ and double positive cells among PCa subtypes.  Treatments with CONV-TOPO, METRO-TOPO and CONV-DTX+METRO-TOPO reduced stemness. METRO-TOPO reduced CD44+ cells to a greater extent compared to CONV-TOPO and CONV-DTX treatments, whereas combination treatment with CONV-DTX+METRO-TOPO reduced stemness (CD44+ cells) the most in ARHigh/mCRPC/NEPC. A) PC-3M, B) DU145 cell lines.
Data from Metronomic Administration of Topotecan Alone and in Combination with Docetaxel Inhibits Epithelial–mesenchymal Transition in Aggressive Variant Prostate Cancers
Authors: Mitra Ghosh Taraswi , Mazumder Suman , Davis Joshua , Yadav Jyoti , Akinpelu Ayuba , Alnaim Ahmed , Kumar Harish , Waliagha Razan , Church Bird Allison E. , Rais-Bahrami Soroush , Bird R. Curtis , Mistriotis Panagiotis , Mishra Amarjit , Yates Clayton C. , Mitra Amit K. , Arnold Robert D.
Published: July 2023

Prostate cancer is the second leading cause of noncutaneous cancer-related deaths in American men. Androgen deprivation therapy (ADT), radical prostatectomy, and radiotherapy remain the primary treatment for patients with early-stage prostate cancer (castration-sensitive prostate cancer). Following ADT, many patients ultimately develop metastatic castration-resistant prostate cancer (mCRPC). Standard chemotherapy options for CRPC are docetaxel (DTX) and cabazitaxel, which increase median survival, although the development of resistance is common. Cancer stem-like cells possess mesenchymal phenotypes [epithelial-to-mesenchymal transition (EMT)] and play crucial roles in tumor initiation and progression of mCRPC. We have shown that low-dose continuous administration of topotecan (METRO-TOPO) inhibits prostate cancer growth by interfering with key cancer pathway genes. This study utilized bulk and single-cell or whole-transcriptome analysis [(RNA sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq)], and we observed greater expression of several EMT markers, including Vimentin, hyaluronan synthase-3, S100 calcium binding protein A6, TGFB1, CD44, CD55, and CD109 in European American and African American aggressive variant prostate cancer (AVPC) subtypes—mCRPC, neuroendocrine variant (NEPC), and taxane-resistant. The taxane-resistant gene FSCN1 was also expressed highly in single-cell subclonal populations in mCRPC. Furthermore, metronomic-topotecan single agent and combinations with DTX downregulated these EMT markers as well as CD44+ and CD44+/CD133+ “stem-like” cell populations. A microfluidic chip-based cell invasion assay revealed that METRO-TOPO treatment as a single agent or in combination with DTX was potentially effective against invasive prostate cancer spread. Our RNA-seq and scRNA-seq analysis were supported by in silico and in vitro studies, suggesting METRO-TOPO combined with DTX may inhibit oncogenic progression by reducing cancer stemness in AVPC through the inhibition of EMT markers and multiple oncogenic factors/pathways.
Significance:
The utilization of metronomic-like dosing regimens of topotecan alone and in combination with DTX resulted in the suppression of makers associated with EMT and stem-like cell populations in AVPC models. The identification of molecular signatures and their potential to serve as novel biomarkers for monitoring treatment efficacy and disease progression response to treatment efficacy and disease progression were achieved using bulk RNA-seq and single-cell-omics methodologies.
Data from A Th1-like CD4+ T-cell Cluster That Predicts Disease-free Survival in Early-stage Lung Cancer
Authors: Yanagihara Akitoshi , Yamasaki Satoshi , Hashimoto Kosuke , Taguchi Ryo , Umesaki Tetsuya , Imai Hisao , Kaira Kyoichi , Nitanda Hiroyuki , Sakaguchi Hirozo , Ishida Hironori , Kobayashi Kunihiko , Horimoto Katsuhisa , Kagamu Hiroshi
Published: July 2023

Perioperative immune checkpoint inhibitors have been shown to improve prognosis in early-stage lung cancer. However, no biomarkers are known to indicate the requirement for treatment. This study aimed to identify T-cell clusters responsible for antitumor immunity in patients with early-stage lung cancer. Preoperative blood samples from 50 consecutive patients with lung cancer who were diagnosed as operable and underwent complete resection were analyzed by mass cytometry. Patients were divided into two groups: no recurrence at a minimum observation period of 851 days (median observation period: 1,031.5 days) and recurrence by the last observation date. Mass cytometry and single-cell RNA sequencing analysis of lymph nodes (LN) and tumor-infiltrating T cells were also performed. CCR4−CCR6+ Th7R showed discriminative ability between recurrence and non-recurrence patients with lung cancer. Patients with more than 3.04% Th7R showed significantly favorable disease-free survival. Th7R was a major component of CD4+ T cells in tumor microenvironments and LNs adjacent to lung cancer tissues and was the only cluster that decreased in peripheral blood after the removal of cancer tissues, suggesting that Th7R was primed and proliferated in tumor-draining LNs in the presence of cancer tissues. Th7R had the kinetics that antitumor T cells should have, as indicated by the cancer immunity cycle; thus, peripheral blood Th7R could represent the potency of tumor immunity by reflecting priming and proliferation in tumor-draining LNs and Th7R in the tumor microenvironment. Prediction using peripheral Th7R before surgery could allow the selection of patients who require perioperative drug therapy and optimize therapeutic interventions with clinical implications.
Significance:
Peripheral Th7R, a Th1-like CD4+ T-cell cluster reflecting priming status in draining LNs and immune status in the tumor microenvironment, predicts disease-free survival after complete resection and has significant clinical relevance in selecting appropriate therapeutic interventions in patients with early-stage lung cancer.
Confidence is predicted by pre- and post-choice decision signal dynamics
Authors: Grogan John P. , Rys Wouter , Kelly Simon P. , O’Connell Redmond G.
Published: July 2023

It is well established that one’s confidence in a choice can be influenced by new evidence encountered after commitment has been reached, but the processes through which post-choice evidence is sampled remain unclear. To investigate this, we traced the pre- and post-choice dynamics of electrophysiological signatures of evidence accumulation (Centro-parietal Positivity, CPP) and motor preparation (mu/beta band) to determine their sensitivity to participants’ confidence in their perceptual discriminations. Pre-choice CPP amplitudes scaled with confidence both when confidence was reported simultaneously with choice, and when reported 1 second after the initial direction decision with no intervening evidence. When additional evidence was presented during the post-choice delay period, the CPP exhibited sustained activation after the initial choice, with a more prolonged build-up on trials with lower certainty in the alternative that was finally endorsed, irrespective of whether this entailed a change-of-mind from the initial choice or not. Further investigation established that this pattern was accompanied by later lateralisation of motor preparation signals toward the ultimately chosen response and slower confidence reports when participants indicated low certainty in this response. These observations are consistent with certainty-dependent stopping theories according to which post-choice evidence accumulation ceases when a criterion level of certainty in a choice alternative has been reached, but continues otherwise. Our findings have implications for current models of choice confidence, and predictions they may make about EEG signatures.
Re-Examining the Industry Experience–Venture Survival Relationship
Authors: Guenther Agnes , Shaver J. Myles
Published: July 2023

We re-examine the finding that new ventures employing individuals with industry experience have survival advantages and conclude that it is unlikely a reflection of the underlying theoretical mechanism advanced in the literature—individuals applying their industry-specific knowledge. We come to this conclusion by leveraging detailed linked employer-employee data from Denmark and conducting an inferred pattern analysis where we analyze several empirical relationships that we interpret in tandem. After replicating the industry experience–venture survival relationship, we identify several empirical puzzles if the underlying causal mechanism is leveraging industry-specific knowledge. In light of these puzzles, analysis of their robustness, and initial exploratory empirical investigations, we propose that other human capital characteristics that correlate with industry experience (i.e., overall experience and wages) are better explanatory factors. The analysis illustrates how the data-grounded steps of an inferred pattern analysis can guide future theoretical development and empirical investigations to identify the causal mechanism underlying a well-established empirical relationship in the literature.

Supplemental Material: The online appendix is available at https://doi.org/10.1287/stsc.2022.0033 .
Nursing care of the hospitalised eye case
Authors: Johns Imogen
Published: July 2023

Many horses hospitalized for eye problems become long-term patients, often spending weeks and, on occasions, months in hospital. Nursing care of these patients is critical not only for the successful outcome of their eye condition, but also for ensuring that when they do return to their owner's care, they are happy, healthy horses. Key factors contributing to these outcomes include recognition of ocular pain, helping horses cope with limited vision and management of subpalpebral lavage systems, including recognition of complications and ‘trouble-shooting’. Other important factors include behavioural modification to help maintain a ‘happy’ patient (including environmental enrichment), general husbandry, monitoring for colic and attention to nutrition to avoid weight changes.
Macrofungal diversity in small-holder oil palm plantations on tropical peatlands
Authors: EKYASTUTI WIWIK , ASTIANI DWI , EKAMAWANTI HANNA ARTUTI , JAMIAT JAMIAT
Published: July 2023

Abstract. Ekyastuti W, Astiani D, Ekamawanti HA, Jamiat. 2023. Macrofungal diversity in small-holder oil palm plantations on tropical peatlands. Biodiversitas 24: 3454-3461. Mushrooms play an important role in ecosystems and human life. They can be found in various places including in oil palm plantations. The aim of this study was to survey and identify the population of macroscopic mushrooms or macrofungi in small-holder oil palm plantations on tropical peatlands in Kampung Baru Village, Kubu Raya District, West Kalimantan, Indonesia using the field survey method. In the research area, a dam has been constructed to regulate the water level at 30 cm, 40 cm, 50 cm, and 60 cm from the surface. Sampling was carried out in these four conditions. The study revealed the presence of 34 species of macrofungi belonging to the Basidiomycota. A groundwater level ranging from 40-60 cm from the surface was identified as favorable for macrofungal growth. Meanwhile, a groundwater level of 30 cm from the surface was found to be unsuitable for macrofungal growth in the peatlands. The level of macrofungal species diversity in small-holder oil palm plantations on the peatlands was determined to be moderate.
Cor de pele e orientação sexual
Authors: Oliveira Ribeiro Pedro Eduardo
Published: July 2023

Com um consumo crescente de notícias nas redes sociais em Portugal (Cardoso, Baldi, Paisana et al., 2021) e uma presença crescente do jornalismo no digital (Salaverría, 2019), as capas das edições físicas têm também vindo a ser partilhadas naquelas. Enquanto todos de informação, as capas produzem sentidos e discursos. Visando tal comprovar, procedeu-se, portanto, aos contributos da Semiótica Social e dos Estudos do Discurso para analisar duas capas de jornais e quatro publicações no Facebook e no Twitter, com alcance relevante. Conclui-se sobretudo que os sentidos produzidos pelas capas sobre as pessoas nelas incluídas são: da parte do Record, interventivo, e da parte do Tal&Qual, depreciativo. Quanto aos discursos produzidos em torno delas com as publicações, destaca-se uma convergência com os sentidos produzidos pela capa, no caso do Record, e uma divergência com os que são produzidos com a capa do Tal&Qual.
Graded weakly 1-absorbing primary ideals
Authors: Bataineh Malik , Abu-Dawwas Rashid
Published: July 2023

Let

G

G

be a group and

R

R

be a

G

G

-graded commutative ring with nonzero unity 1. In this article, we introduce the concept of graded weakly 1-absorbing primary ideals which is a generalization of graded 1-absorbing primary ideal. A proper graded ideal

P

P

of

R

R

is said to be a graded weakly 1-absorbing primary ideal of

R

R

if whenever nonunit elements

x
,
y
,
z
∈
h

(

R

)

x,y,z\in h\left(R)

such that

0
≠
x
y
z
∈
P

0\ne xyz\in P

, then

x
y
∈
P

xy\in P

or

z

n

∈
P

{z}^{n}\in P

, for some

n
∈
N

n\in {\mathbb{N}}

. Several properties of graded weakly 1-absorbing primary ideals are investigated.
Effect of a CrossMab cotargeting CD20 and HLA-DR in non-Hodgkin lymphoma
Authors: Zeng Jing , Chen Yalin , Zhang Tingting , Wang Jinjing , Yang Yan , Fang Yi
Published: July 2023

Objectives

To evaluate the anti-tumor activities of CD20/HLA-DR CrossmabCH1-CL through cell and animal models.

Methods

Based on “knobs-into-holes” and “crossover” technology, CrossMab, targeting CD20 and HLA-DR, was constructed. A binding assay and a competitive inhibition assay were performed to confirm its specificity. The effects of CrossMab on antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity were measured. Cell apoptosis, lysosome-mediated cell death, and lysosomal permeability were quantified. In addition, the effects of CrossMab on peripheral blood leukocytes were tested. The pharmacokinetics were determined with a noncompartmental analysis model. Human malignant lymphoma xenograft models in CB17-SCID mice were established for an in-vivo efficacy study.

Results

The antitumor activities of CrossMab were shown both in vitro and in vivo. CrossMab exhibited strong binding to CD20 and HLA-DR at the same time in Raji cells. CrossMab also demonstrated antilymphoma effects by inducing antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity. Furthermore, CrossMab altered the lysosomal membrane permeability. The toxicity of CrossMab on normal peripheral blood lymphocytes (PBLs) was specific to B cells. A mouse xenograft model demonstrated the antitumor activities of CrossMab in vivo.

Conclusions

CrossMab exhibited an enhanced antigen recognition ability and antitumor activities in lymphoma without evident toxicity. CrossMab could be an effective immunotherapeutic strategy for non-Hodgkin lymphoma.
Base editing therapy forges ahead
Authors: Jiang Wen , Yang Rui
Published: July 2023

CRISPR/Cas-based gene editing is an innovative biotechnology that has revolutionized genetic engineering in recent years. The process involves induction of a double-strand break (DSB) at the desired DNA site and subsequent cellular repair. Two primary mechanisms drive DSB repair: non-homologous end joining and homologous recombination-mediated repair. Non-homologous end joining, the primary mode of DSB repair, is a simple high-efficiency process that is susceptible to errors, and unpredictable nucleotide insertion or deletion mutations. In contrast, point mutations account for more than 50% of human genetic disorders and are the most frequent type of genetic variation in nature. Base editing is a precise gene editing approach in which a single DNA base is substituted without introduction of DSBs or use of a repair template. This technique has promising therapeutic potential in gene therapy, owing to its high efficiency and controllable editing results. Since the invention of the first base editing tools, the technique has rapidly developed and undergone clinical trials. This review summarizes progress in gene therapy through base editing, including DNA and RNA base editing, with particular emphasis on recent clinical trial and preclinical research advancements, current limitations and remaining challenges, and prospects for further research and applications.
Historia Caribe Vol. 14 Núm. 35 (2019): Julio-Diciembre
Authors: Editorial Comité
Published: July 2023

Historia Caribe Vol. 14 Núm. 35 (2019): Julio-Diciembre
Historia Caribe Vol. 16 Núm. 38 (2021): Enero-Junio
Authors: Editorial Comité
Published: July 2023

Historia Caribe Vol. 16 Núm. 38 (2021): Enero-Junio
La restauración del orden. Civiles y militares en la sublevación O’Higginista de 1826
Authors: Verbal Stockmeyer Valentina
Published: July 2023

Este artículo estudia una importante insubordinación civil-militar del periodo de organización de la república en Chile (1823-1830), llevado a cabo por el bando o’higginista, que era partidario del retorno, desde Lima, de Bernardo O’Higgins al poder. A diferencia de algunos autores referidos en estas páginas, este trabajo apunta también a relevar la existencia y naturaleza del militarismo de la temprana república chilena.
Vestir el cuerpo político. La indumentaria de las mujeres libres en Cartagena, Portobelo y Valledupar, 1792-1807
Authors: Conde Calderón Jorge , Álvarez Hernández Lea
Published: July 2023

Este artículo analiza el uso de vestidos de seda y objetos lujosos entre las mujeres de la plebe en tres ciudades del Nuevo Reino de Granada: Cartagena, Portobelo y Valledupar. El hecho hace parte de los cambios ocurridos tanto en el cuerpo político como en el social a finales del siglo XVIII e inicios del XIX. Ello originó controvertidas reacciones de las autoridades españolas y las mujeres blancas, quienes no aceptaban que pardas y mulatas lucieran una indumentaria similar a la que ellas siempre habían utilizado. Igualmente, afirma que la vestimenta y objetos lujosos eran un medio adecuado de comunicación política que permitía una expresiva, rica y participativa cultura plebeya.
La multiforme identidad protestante en Latinoamérica en el siglo XX
Authors: Ramírez Suárez Luis Eduardo
Published: July 2023

Para entender el fenómeno del protestantismo es importante reconocer el ambiente político que lo propició y la influencia teológica en su formación y praxis. El presente artículo aborda, mediante una investigación bibliográfica y documental, como se forjaron las diferentes expresiones del protestantismo latinoamericano durante el siglo XX bajo la influencia de movimientos teológicos propiciados por los Congresos Misioneros Mundiales de Edimburgo y Panamá, el movimiento pentecostal, y los cambios en la política estadounidense. Estos movimientos enriquecieron el protestantismo latinoamericano como un movimiento multiforme con una teología y praxis dinámicas que permitieron su contextualización y el desarrollo de su identidad.
Social Movement Madres de Soacha (Mothers of Soacha)
Authors: González-Malabet María , González Arana Roberto
Published: July 2023

This article analyzes the history of the Madres de Soacha, a social movement of mothers that searched for the truth about the extra-judicial killings of their sons in 2008. After a judicial battle, these mothers proved that the Colombian Army perpetuated these massacres under the state policy of Democratic Security. This research argues for a robust notion of collective identity and memory building of this female movement, which is not reduced to a mere concept of victims. It displays different national and international actions that the Madres de Soacha developed for building memory and collective identity through narratives and agency.
An Analysis of Trauma Team Activations at a South African Level One Trauma Centre
Authors: Goldsteini Lara Nicole , Beskyd Peter , Wells Mike
Published: July 2023

Introduction: South Africa has a high prevalence of trauma causing a significant healthcare and financial burden. Various scoring systems have been developed to guide trauma team activation (TTA). To date, however, there are no standardised criteria for TTA. The aim of this preliminary study was to analyse the practice of clinical judgement TTA when compared to the American College of Surgeons Committee on Trauma (ACS-COT) TTA guideline and the Loma Linda Rule for TTA.

Methods: This was a retrospective analysis of trauma registry data over a one-year period from a Level 1 trauma centre in South Africa.

Results: There were 9207 trauma patients that presented to the Emergency Department during the 1-year study period. The prevalence of trauma team activation by clinical judgement was 9.4%. Only 407 patients (48%) of the clinical judgement TTAs fulfilled the ACS-COT guidelines for TTA. Using the Loma Linda Rule, 456 patients (53.8%) of the clinical judgement TTAs fulfilled TTA criteria. Despite the over-triage by the clinical judgement TTA, almost two-thirds of the TTA patients were admitted to the intensive care or high care units.

Conclusions: This preliminary study showed that TTA using healthcare provider clinical judgement resulted in trauma team over-activation when compared to the ACS-COT guideline and the Loma Linda Rule. Over-activation of the trauma team consumes valuable resources and potentially endangers those patients who actually need TTA and cannot receive it. Implementation of a standardised TTA tool could potentially aid in the optimisation of patient care and appropriate resource utilisation.
Tüketicilerin satın alma alışkanlıkları: Ankara, Etimesgut örneği
Authors: YANĞIÇ YÜKSEL Çile , KARAGÖZLÜ Nural
Published: July 2023

Amaç: Bu çalışmanın amacı, Ankara ili Etimesgut ilçesinde yaşayan tüketicilerin yaş, cinsiyet, gelir düzeyi, eğitim seviyesi gibi farklı demografik özelliklerle oluşan bilgi birikimlerinin gıda maddelerini satın alma alışkanlıkları arasındaki ilişkinin saptanmaya çalışılmasıdır.
Materyal ve Yöntem: Farklı demografik özelliklere sahip 517 yetişkin bireyle yapılan anket çalışmasından sağlanan veriler kullanılmış; demografik özellikler ile gıda satın alma alışkanlıkları ve tüketicilerin gıda maddelerini satın aldıkları yerler incelenmiştir. Elde edilen veriler SPSS programı ve ki-kare (χ2) testi uygulanarak değerlendirilmiştir.
Araştırma Bulguları: Çalışma sonunda tüketicilerin eğitim durumu ile alışveriş sırasında ambalajın zarar görüp görmemesine dikkat etme (p<0,01) ve cinsiyet ile ürünün etiket bilgileri ve alerjen madde içerip içermeme özellikleri arasındaki ilişkiler anlamlı bulunmuştur (p<0,05). Tüketicilerin meyve-sebze gibi ürünleri satın alırken semt pazarlarını tercih ettikleri, “sokak satıcısı”nı ise en düşük güvenilirliğe sahip olarak düşündükleri saptanmıştır.
Sonuç: Alışveriş sırasında özellikle son kullanma tarihine, ambalaj materyalinin zarar görüp görmemesine dikkat edilmesi; satın alma alışkanlıklarının en önemli parametrelerini oluşturmaktadır. Tüketicilerin gıdaya ulaşma noktası olarak kendi ürünlerini ve süpermarketleri tercih etmeleri devlet kanalıyla gıda kontrol mekanizmasının doğru ve yeterli olarak işlemesinin önemini ortaya koymaktadır.
Application of Convolutional Neural Network to Gripping Comfort Evaluation Using Gripping Posture Image
Authors: Hokari Kazuki , Ikarashi Makoto , Pramudita Jonas A. , Okada Kazuya , Ito Masato , Tanabe Yuji
Published: July 2023

Gripping comfort evaluation was crucial for designing a product with good gripping comfort. In this study, a novel evaluation method using gripping posture image was constructed based on convolutional neural network (CNN). Human subject experiment was conducted to acquire gripping comfort scores and gripping posture images while gripping seven objects with simple shape and eleven manufactured products. The scores and the images were used as training set and validation set for CNN. Classification problem was employed to classify gripping posture images as comfort or discomfort. As a result, accuracies were 91.4% for simple shape objects and 76.2% for manufactured products. Regression problem was utilized to predict gripping comfort scores from gripping posture images while gripping cylindrical object. Gripping posture images of radial and dorsal sides in direction of hand were used to investigate effect of direction of hand on prediction accuracy. Consequently, mean absolute errors (MAE) of gripping comfort scores were 0.132 for radial side and 0.157 for dorsal side in direction of hand. In both problems, the results indicated that these evaluation methods were useful to evaluate gripping comfort. The evaluation methods help designers to evaluate products and enhance gripping comfort.
Blockade of TGF-β signalling alleviates human adipose stem cell senescence induced by native ECM in obesity visceral white adipose tissue
Authors: han xueya , Li Weihong , He Xu , Lu Xin , Zhang Yu , Li Yaqiong , Bi Guoyun , Ma Xuqing , Huang Xiaowu , Bai Rixing , Zhang Haiyan
Published: July 2023

Background
Abdominal obesity is appreciated as a major player in insulin resistance and metabolically dysfunctional adipose tissue. Inappropriate extracellular matrix (ECM) remodeling and functional alterations in human adipose stromal/stem cells (hASCs) have been linked with visceral white adipose tissue (vWAT) dysfunction in obesity. Understanding the interactions between hASCs and the native ECM environment in obese vWAT is required for the development of future therapeutic approaches for obesity-associated metabolic complications.
Methods
The phenotypes and transcriptome properties of hASCs from the vWAT of obese patients and lean donors were assessed. The hASC-derived matrix from vWAT of obese or lean patients was generated in vitro using a decellularized method. The topography and the major components of the hASC-derived matrix were determined. The effects of the obese hASC-derived matrix on cell senescence and mitochondrial function were further determined.
Results
We showed that hASCs derived from the vWAT of obese patients exhibited senescence and were accompanied by the increased production of ECM. The matrix secreted by obese hASCs formed a fibrillar suprastructure with an abundance of fibronectin, type I collagen, and transforming growth factor beta 1 (TGF-β1), which resembles the native matrix microenvironment of hASCs in visceral WAT derived from obese patients. Furthermore, the obese hASC-derived matrix promoted lean hASC ageing and induced mitochondrial dysfunction compared to the lean hASC-derived matrix. Blockade of TGF-β1 signalling using a TGF-β1 neutralizing antibody alleviated the lean hASC senescence and mitochondrial dysfunction induced by the obese hASC-derived matrix.
Conclusions
Native ECM in obesity vWAT initiates hASC senescence through TGF-β1-mediated mitochondrial dysfunction. These data provide a key mechanism for understanding the importance of cell-ECM interactions in hASCs senescence in obesity.
Polygenic risk alters the penetrance of monogenic kidney disease
Authors: Kiryluk Krzysztof , Khan Atlas , Shang Ning , Nestor Jordan , Weng Chunhua , Hripcsak George , Harris Peter , Gharavi Ali
Published: July 2023

Importance
Chronic kidney disease (CKD) is a genetically complex disease determined by an interplay of monogenic, polygenic, and environmental risks. The most common forms of monogenic kidney disorders include autosomal dominant polycystic kidney disease (ADPKD), caused by mutations in the PKD1 or PKD2 genes, and COL4A-associated nephropathy (COL4A-AN), caused by mutations in COL4A3, COL4A4, or COL4A5 genes. These disorders have incomplete penetrance and variable expressivity. It is presently unknown if some of the variability in penetrance can be attributed to polygenic factors.
Objective
Our objectives were to investigate the interplay of polygenic and monogenic risk of kidney disease and to test the utility of polygenic risk scores for risk stratification in ADPKD and COL4-AN.
Design
We combined exome/genome sequencing, SNP micro-array, and electronic health record data from the UK Biobank (N = 469,835 participants) and the All of Us (N = 98,622 participants) datasets to test the effects of the genome-wide polygenic score (GPS) for CKD in ADPKD and COL4-AN variant carriers defined by strict variant classification criteria. We used the eMERGE-III electronic CKD phenotype to define cases (estimated glomerular filtration rate (eGFR) < 60 mL/min/1.73m2 or kidney failure) and controls (eGFR > 90 mL/min/1.73m2 in the absence of kidney disease diagnoses). The GPS was tested as a predictor of CKD in qualifying variant carriers and non-carriers using logistic regression after adjustment for age, sex, diabetes, and genetic ancestry.
Results
The GPS was predictive of CKD in ADPKD variant carriers (ORmeta=2.28 per SD, 95%CI: 1.55–3.37, P = 2.6E-05) as well as non-carriers (ORmeta=1.72 per SD, 95% CI = 1.69–1.76, P < E-300) independent of age, sex, diabetes, and genetic ancestry. Compared to the middle tertile of the GPS distribution for non-carriers, ADPKD variant carriers in the top tertile had a 54-fold increased risk of CKD, while ADPKD variant carriers in the bottom tertile had only a 3-fold increased risk of CKD. Similarly, the GPS was predictive of CKD in both COL4-AN carriers (ORmeta=1.78, 95% CI = 1.22–2.58, P = 2.38E-03) and non-carriers (ORmeta=1.70, 95%CI: 1.68–1.73 P < E-300). The carriers in the top GPS tertile had a 2.5-fold higher risk of CKD while the risk for carriers in the bottom GPS tertile was not different from the average population risk.
Conclusions and Relevance
Variable penetrance of kidney disease in ADPKD and COL4-AN is partially explained by the differences in polygenic risk profiles. Accounting for polygenic factors improves risk stratification in monogenic kidney disease and may have clinical implications for genetic counseling.
Distortion correction using topup algorithm by single k- space (TASK) for echo planar imaging
Authors: Hwang Seon-Ha , Lee Hyun-Soo , Choi Seung Hong , Park Sung-Hong
Published: July 2023

Distortion of echo planar imaging (EPI) can be corrected using B0 field maps, which can be acquired with the topup algorithm that requires two EPI images with opposite distortions. In this study, we propose a new algorithm, termed Topup Algorithm by Single K-space (TASK), to generate two input images from a single k-space for the topup algorithm to correct EPI distortions. The centric EPI contains the opposite phase-encoding polarities in one k-space, which can be divided into two halves with opposite distortions. Therefore, two inputs could be extracted by dividing the k-space into halves and processing them using the proposed procedure including an iterative procedure of automatic brain masking and uniformity correction. The efficiency of TASK was evaluated using 3D EPI. Quantitative evaluations showed that TASK corrected EPI distortion in a similar level to the traditional methods. The estimated field maps from the conventional topup and TASK showed high correlation (\(r=0.8048\pm 0.0534\)). An ablation study showed the validity of every suggested step. Furthermore, it was confirmed that TASK was effective for distortion correction of two-shot centric EPI as well, demonstrating its wider applicability. In conclusion, TASK can correct EPI distortions by its own single k-space information with no additional scan.
Lineament depth deduction using geomorphometry and 3d-Euler solution
Authors: koundi Mourad El , DHAOUI Mohamed , GHALI Abdessalem EL
Published: July 2023

In this paper, we present a novel method for determining the depth of lineaments using satellite gravity data, which we test in the Sahel of Sfax area (eastern Tunisia). This region has a relatively flat topography with few significant discontinuities at the surface.
As a first step, we used morphometric indices such as slope, aspect, and hydrographic network, calculated from SRTM-DEM, to generate separate lineament maps. We then combined these lineaments into a synthetic lineament map, which showed a high level of agreement with the Bouguer anomaly map derived from satellite gravity data and with previous studies that used geophysical data (seismic and gravity). We also validated the lineament map using field data.
By fusing these lineaments with the 3D Euler solution map, we were able to deduce the depth classification of the lineaments. Our results demonstrate the effectiveness of our proposed method in estimating the depth of lineaments and its potential to provide valuable insights into the subsurface geology of an area. This method can be a useful tool for mineral exploration, hydrocarbon exploration, and geohazard assessments.
Transforming a cordon toll ring to congestion charging scheme: The impacts in the case of Bergen two years on
Authors: Odeck James , Presterud Eirik Lund
Published: July 2023

This paper assesses how transforming a cordon toll ring with flat toll rates to a congestion charging scheme works in achieving its intended objectives. The contribution to the literature on transportation is that few studies have examined how such transformations fulfill their main policy objectives. We use the City of Bergen in Norway as a case study to infer the extent to which policy objectives were achieved two years later. In early 2016, the city council transformed its flat-rate cordon toll ring into a congestion charging scheme. It entailed an increase in toll rates during rush hour and a decrease during non-rush hour periods. The primary aim was to reduce traffic levels entering the city center during rush hour and improve the inner city's air quality. A secondary objective was to maintain income at the current level to complete the preplanned and already sanctioned investment projects. We use traffic counts, NO2 and PM10 emission measurements, and annual toll income to evaluate how the congestion scheme performed two years after implementation. We find that (i) traffic levels during rush hour and non-rush hour periods were reduced by 12% and 2%, respectively, giving a grand reduction of 6%; (ii) the emissions in NO2 and PM10 were reduced by 6.3% and 11%, respectively; (iii) the toll income dropped by 13%, meaning that preplanned investments would not be implemented on time; and (iv) the use of other modes of transportation increased. Overall, congestion charging worked according to transportation economists' longstanding suggestions.