bluuebunny/crossref_metadata_2025 huggingface dataset:
The Association of IFN-γ, TNF-α, and Interleukins in Bronchoalveolar Lavage Fluid with Lung Cancer: A Prospective Analysis
Authors: Hogea Patricia , Tudorache Emanuela , Fira-Mladinescu Ovidiu , Marc Monica , Manolescu Diana , Bratosin Felix , Rosca Ovidiu , Mavrea Adelina , Oancea Cristian
Published: June 2023

Lung cancer is a leading cause of cancer-related mortality worldwide. Identifying novel diagnostic and prognostic biomarkers is essential for improving patient outcomes. This study aimed to investigate the predictive role of cytokines from bronchoalveolar lavage fluid (BALF) in lung cancer diagnosis and prognosis. A prospective study was conducted on 33 patients with suspected lung cancer, divided into inflammatory and non-inflammatory BALF groups. Inflammatory markers in BALF were assessed, and their association with lung cancer risk was analyzed using receiver operating characteristic (ROC) plot analysis, sensitivity and specificity percentages, and regression analysis. Statistically significant differences were observed between the inflammatory and non-inflammatory groups for several inflammatory markers, including IFN-gamma, IL-1b, IL-2, IL-6, IL-10, and IL-12p70. In the follow-up analysis, significant differences persisted for IFN-gamma, IL-1b, IL-2, IL-4, and IL-6. ROC plot analysis revealed that IL-12p70 had the highest area under the curve (AUC) value (0.702), followed by IL-2 (0.682), IL-6 (0.620), IL-4 (0.611), TNF-alpha (0.609), IL-10 (0.604), IL-1b (0.635), and IFN-gamma (0.521). IL-6 showed the highest sensitivity (73%), and IL-1b had the highest specificity (69%). Regression analysis demonstrated that IL-6 (cut-off = 25 pg/mL) and IL-12p70 (cut-off = 30 pg/mL) had the highest odds ratios for lung cancer risk, at 5.09 (95% CI: 2.38–9.24, p < 0.001) and 4.31 (95% CI: 1.85–8.16, p < 0.001), respectively. Cytokines from BALF, particularly IL-6 and IL-12p70, show potential as diagnostic and prognostic biomarkers for lung cancer. Further studies with larger cohorts are warranted to confirm these findings and elucidate the clinical implications of these markers in lung cancer management.
Corporate Finance, Governance, and Social Responsibility
Authors: Gherghina Ștefan Cristian
Published: June 2023

Corporate finance is a branch of finance that focuses on how companies handle their cash flow, raise capital, make investments, and implement accounting systems [...]
Frontal Fibrosing Alopecia: An Observational Single-Center Study of 306 Cases
Authors: Carmona-Rodríguez Marcos , Moro-Bolado Fernando , Romero-Aguilera Guillermo , Ruiz-Villaverde Ricardo , Carriel Víctor
Published: June 2023

(1) Background: Frontal fibrosing alopecia (FFA) is a scarring alopecia that predominantly affects postmenopausal women; (2) Methods: A retrospective, observational, single-center study was conducted in the Hospital General Universitario in Ciudad Real, Spain, including all patients diagnosed with FFA between 2010 and 2021; (3) Results: A total of 306 patients (296 women and 10 men) were included in our study. The mean age of onset was 59.5 years. The severity of this disease was evenly distributed between mild (147 patients) and severe (149 patients) forms. There was a positive, statistically significant, medium correlation between the severity of the disease and its time of progression. Moreover, hypothyroidism was present in 70 patients (22.9%) and classic signs of concomitant lichen planopilaris were observed in just 30 patients (9.8%), while other forms of lichen planus were uncommon. The estimated prevalence in our population is 0.15% and the incidence is 15.47 new cases per 100,000 inhabitants; (4) Conclusions: The time of progression was positively correlated with the severity of FFA. However, the presence of clinical signs, such as inflammatory trichoscopic signs, was not associated with the progression of this condition.
Bacterial Contamination of Healthcare Students’ Mobile Phones: Impact of Specific Absorption Rate (SAR), Users’ Demographics and Device Characteristics on Bacterial Load
Authors: Maurici Massimo , Pica Francesca , D’Alò Gian Loreto , Cicciarella Modica Domenico , Distefano Alessandra , Gorjao Margarida , Simonelli Maria Sofia , Serafinelli Livio , De Filippis Patrizia
Published: June 2023

We quantitatively and qualitatively evaluated the bacterial contamination of mobile phones (MPs) in relation to users’ demographics, habits, and device characteristics by administering questionnaires to 83 healthcare university students and sampling their MPs by following a cross-sectional design. The heterotrophic plate count (HPC) at 22 °C (HPC 22 °C) and 37 °C (HPC 37 °C), Enterococci, Gram-negative bacteria, and Staphylococci were evaluated. Higher bacterial loads were detected for HPC 37 °C and Staphylococci (416 and 442 CFU/dm2, respectively), followed by HPC 22 °C, Enterococci, and Gram-negative bacteria; the vast majority of samples were positive for HPC 37 °C, HPC 22 °C, and Staphylococci (98%), while Enterococci (66%) and Gram-negative bacteria (17%) were detected less frequently. A statistically significant positive correlation (r = 0.262, p < 0.02) was found between the European head specific absorption rate (SAR) and both HPC 37 °C and Staphylococci; Enterococci showed a strong, significant correlation with HPC 37 °C, HPC 22 °C, and Gram-negative bacteria (r = 0.633, 0.684, 0.884) and a moderate significant correlation with Staphylococci (r = 0.390). Significant differences were found between HPC 22 °C and the type of internship attendance, with higher loads for Medicine. Students with a daily internship attendance had higher HPC 22 °C levels than those attending <6 days/week. Our study showed that bacteria can survive on surfaces for long periods, depending on the user’s habits and the device’s characteristics.
Studying Consensus and Disagreement during Problem Solving in Teams through Learning and Response Generation Agents Model
Authors: Doboli Alex , Curiac Daniel-Ioan
Published: June 2023

Understanding the process of reaching consensus or disagreement between the members of a team is critical in many situations. Consensus and disagreement can refer to various aspects, such as requirements that are collectively perceived to be important, shared goals, and solutions that are jointly considered to be realistic and effective. Getting insight on how the end result of the interaction process is influenced by parameters such as the similarity of the participants’ experience and behavior (e.g., their available concepts, the produced responses and their utility, the preferred response generation method, and so on) is important for optimizing team performance and for devising novel applications, i.e., systems for tutoring or self-improvement and smart human computer interfaces. However, understanding the process of reaching consensus or disagreement in teams raises a number of challenges as participants interact with each other through verbal communications that express new ideas created based on their experience, goals, and input from other participants. Social and emotional cues during interaction are important too. This paper presents a new model, called Learning and Response Generating Agents, for studying the interaction process during problem solving in small teams. As compared to similar work, the model, grounded in work in psychology and sociology, studies consensus and disagreement formation when agents interact with each other through symbolic, dynamically-produced responses with clauses of different types, ambiguity, multiple abstraction levels, and associated emotional intensity and utility.
New Family of Multi-Step Iterative Methods Based on Homotopy Perturbation Technique for Solving Nonlinear Equations
Authors: Saeed Huda J. , Ali Ali Hasan , Menzer Rayene , Poțclean Ana Danca , Arora Himani
Published: June 2023

This research aims to propose a new family of one-parameter multi-step iterative methods that combine the homotopy perturbation method with a quadrature formula for solving nonlinear equations. The proposed methods are based on a higher-order convergence scheme that allows for faster and more efficient convergence compared to existing methods. It aims also to demonstrate that the efficiency index of the proposed iterative methods can reach up to 43≈1.587 and 84≈1.681, respectively, indicating a high degree of accuracy and efficiency in solving nonlinear equations. To evaluate the effectiveness of the suggested methods, several numerical examples including their performance are provided and compared with existing methods.
Second-Order Neutral Differential Equations with Distributed Deviating Arguments: Oscillatory Behavior
Authors: Al-Jaser Asma , Qaraad Belgees , Bazighifan Omar , Iambor Loredana Florentina
Published: June 2023

In this paper, new criteria for a class oscillation of second-order delay differential equations with distributed deviating arguments were established. Our method mainly depends on making sharper estimates for the non-oscillatory solutions of the studied equation. By using the Ricati technique and comparison theorems that compare the studied equations with first-order delay differential equations, we obtained new and less restrictive conditions that ensure the oscillation of all solutions of the studied equation. Further, we give an illustrative example.
An Efficient Evolution-Based Technique for Moving Target Search with Unmanned Aircraft Vehicle: Analysis and Validation
Authors: Abdel-Basset Mohamed , Mohamed Reda , Hezam Ibrahim M. , Alshamrani Ahmad M. , Sallam Karam M.
Published: June 2023

Recent advances in technology have led to a surge in interest in unmanned aerial vehicles (UAVs), which are remote-controlled aircraft that rely on cameras or sensors to gather information about their surroundings during flight. A UAV requires a path-planning technique that can swiftly recalculate a viable and quasi-optimal path in flight if a new obstacle or hazard is recognized or if the target is moved during the mission. In brief, the planning of UAV routes might optimize a specific problem determined by the application, such as the moving target problem (MTP), flight time and threats, or multiobjective navigation. The complexity of MTP ranges from NP-hard to NEXP-complete because there are so many probabilistic variables involved. Therefore, it is hard to detect a high-quality solution for this problem using traditional techniques such as differential calculus. Therefore, this paper hybridizes differential evolution (DE) with two newly proposed updating schemes to present a new evolution-based technique named hybrid differential evolution (HDE) for accurately tackling the MTP in a reasonable amount of time. Using Bayesian theory, the MTP can be transformed into an optimization problem by employing the target detection probability as the fitness function. The proposed HDE encodes the search trajectory as a sequence of UAV motion pathways that evolve with increasing the current iteration for finding the near-optimal solution, which could maximize this fitness function. The HDE is extensively compared to the classical DE and several rival optimizers in terms of several performance metrics across four different scenarios with varying degrees of difficulty. This comparison demonstrates the proposal’s superiority in terms of the majority of used performance metrics.
Adapting Strategies for Effective Schistosomiasis Prevention: A Mathematical Modeling Approach
Authors: Tabo Zadoki , Kalinda Chester , Breuer Lutz , Albrecht Christian
Published: June 2023

One of the most deadly neglected tropical diseases known to man is schistosomiasis. Understanding how the disease spreads and evaluating the relevant control strategies are key steps in predicting its spread. We propose a mathematical model to evaluate the potential impact of four strategies: chemotherapy, awareness programs, the mechanical removal of snails and molluscicides, and the impact of a change in temperature on different molluscicide performances based on their half-lives and the length of time they persist in contact with target species. The results show that the recruitment rate of humans and the presence of cercaria and miracidia parasites are crucial factors in disease transmission. However, schistosomiasis can be entirely eradicated by combining all of the four strategies. In the face of climate change and molluscicide degradation, the results show that increasing the temperatures and the number of days a molluscicide persists in the environment before it completely degrades decreases the chemically induced mortality rate of snails while increasing the half-life of different molluscicides increases the death rate of snails. Therefore, eradicating schistosomiasis effectively necessitates a comprehensive integration of all preventative measures. Moreover, regions with different weather patterns and seasonal climates need strategies that have been adapted in terms of the appropriate molluscicide and time intervals for reapplication and effective schistosomiasis control.
The Diagnosability of the Generalized Cartesian Product of Networks
Authors: Chen Meirun , Lin Cheng-Kuan
Published: June 2023

Motivated by two typical ways to construct multiprocessor systems, matching composition networks and cycle composition networks, we generalize the definition of the Cartesian product of networks and consider the classical diagnosability of the generalized Cartesian product of networks (GCPNs). In this paper, we determine the accurate value of the classical diagnosability of the generalized Cartesian product of networks (GCPNs) under the PMC model and the MM* model.
NASCTY: Neuroevolution to Attack Side-Channel Leakages Yielding Convolutional Neural Networks
Authors: Schijlen Fiske , Wu Lichao , Mariot Luca
Published: June 2023

Side-channel analysis (SCA) is a class of attacks on the physical implementation of a cipher, which enables the extraction of confidential key information by exploiting unintended leaks generated by a device. In recent years, researchers have observed that neural networks (NNs) can be utilized to perform highly effective SCA profiling, even against countermeasure-hardened targets. This study investigates a new approach to designing NNs for SCA, called neuroevolution to attack side-channel traces yielding convolutional neural networks (NASCTY-CNNs). This method is based on a genetic algorithm (GA) that evolves the architectural hyperparameters to automatically create CNNs for side-channel analysis. The findings of this research demonstrate that we can achieve performance results comparable to state-of-the-art methods when dealing with desynchronized leakages protected by masking techniques. This indicates that employing similar neuroevolutionary techniques could serve as a promising avenue for further exploration. Moreover, the similarities observed among the constructed neural networks shed light on how NASCTY effectively constructs architectures and addresses the implemented countermeasures.
Point Prevalence Survey of Antimicrobial Use in Selected Tertiary Care Hospitals of Pakistan Using WHO Methodology: Results and Inferences
Authors: Ambreen Saadia , Safdar Numrah , Ikram Aamer , Baig Mirza Zeeshan Iqbal , Farooq Ayesha , Amir Afreenish , Saeed Asim , Sabih Farah , Ahsan Qadeer , Zafar Alia , Mahipala Palitha Gunarathna , Saleem Zikria , Salman Muhammad
Published: June 2023

Background and objectives: The inappropriate use of antibiotics in hospitals can potentially lead to the development and spread of antibiotic resistance, increased mortality, and high economic burden. The objective of the study was to assess current patterns of antibiotic use in leading hospitals of Pakistan. Moreover, the information collected can support in policy-making and hospital interventions aiming to improve antibiotic prescription and use. Methodology and materials: A point prevalence survey was carried out with data abstracted principally from patient medical records from 14 tertiary care hospitals. Data were collected through the standardized online tool KOBO application for smart phones and laptops. For data analysis, SPSS Software was used. The association of risk factors with antimicrobial use was calculated using inferential statistics. Results: Among the surveyed patients, the prevalence of antibiotic use was 75% on average in the selected hospitals. The most common classes of antibiotics prescribed were third-generation cephalosporin (38.5%). Furthermore, 59% of the patients were prescribed one while 32% of the patients were prescribed two antibiotics. Whereas the most common indication for antibiotic use was surgical prophylaxis (33%). There is no antimicrobial guideline or policy for 61.9% of antimicrobials in the respected hospitals. Conclusions: It was observed in the survey that there is an urgent need to review the excessive use of empiric antimicrobials and surgical prophylaxis. Programs should be initiated to address this issue, which includes developing antibiotic guidelines and formularies especially for empiric use as well as implementing antimicrobial stewardship activities.
The Superficial Peroneal Nerve Is at Risk during the “All Inside” Arthroscopic Broström Procedure: A Cadaveric Study
Authors: Kim Sung Hwan , Choi Jae Hyuck , Lee Sang Heon , Lee Young Koo
Published: June 2023

Background: The arthroscopic Broström procedure is a promising treatment for chronic ankle instability. However, little is known regarding the location of the intermediate superficial peroneal nerve at the level of the inferior extensor retinaculum; knowledge about this location is important for procedural safety. The purpose of this cadaveric study was to clarify the anatomical relationship between the intermediate superficial peroneal nerve and the sural nerve at the level of the inferior extensor retinaculum. Methods: Eleven dissections of cadaveric lower extremities were performed. The origin of the experimental three-dimensional axis was defined as the location of the anterolateral portal during ankle arthroscopy. The distances from the standard anterolateral portal to the inferior extensor retinaculum, sural nerve, and intermediate superficial peroneal nerve were measured using an electronic digital caliper. The location of inferior extensor retinaculum, the tract of sural nerve, and intermediate superficial peroneal nerve were checked using average and standard deviations. For the statistical analyses, data are presented as average ± standard deviation, and then they are reported as means and standard deviations. Fisher’s exact test was used to identify statistically significant differences. Results: At the level of the inferior extensor retinaculum, the mean distances from the anterolateral portal to the proximal and distal intermediate superficial peroneal nerve were 15.9 ± 4.1 (range, 11.3–23.0) mm and 30.1 ± 5.5 (range, 20.8–37.9) mm, respectively. The mean distances from the anterolateral portal to the proximal and distal sural nerve were 47.6 ± 5.7 (range, 37.4–57.2) mm and 47.2 ± 4.1 (range, 41.0–51.8) mm), respectively. Conclusions: During the arthroscopic Broström procedure, the intermediate superficial peroneal nerve may be damaged by the anterolateral portal; the proximal and distal parts of the intermediate superficial peroneal nerve were located within 15.9 and 30.1 mm, respectively, at the level of the inferior extensor retinaculum in cadavers. These areas should be considered danger zones during the arthroscopic Broström procedure.
A Social-Ecological Model for Inclusion Processes in Urban Agriculture of Vienna, Austria
Authors: Schaffernicht Sophie Kathrin , Quendler Elisabeth
Published: June 2023

The target group of this study—people with disabilities who work and who are cared for in institutions that offer occupational therapy—is largely absent from the discussions about urban agriculture. When implementing the UN Convention on the Rights of People with Disabilities, education and work are essential aspects, especially for the self-determination of people with disabilities. However, people with disabilities are currently often prevented from truly participating in society. The main research objective of this study lies on the identification on systemic barriers to inclusion into horticultural working life as well as possible solutions. The aim of this study was to find out how inclusion processes could be facilitated in the field of urban agriculture in Vienna. A pilot project was carried out for this purpose during May and October 2019. A university, three social institutions with seven employees, two horticultural farms with two farm managers and fifteen people with disabilities took part in this pilot project. During the tomato harvest, action research was applied, evaluated and modified. At the same time, the authors tried to contribute to Grounded Theory. One of the results of this study is the suggestion that offers in the field of urban agriculture should go beyond pure cooperation between social institutions and horticultural farms. A social-ecological scenario with agro-ecological inclusion farms is elaborated. It is concluded that the establishment of such farms in terms of the UN Convention on the Rights of People with Disabilities in Vienna could bring the city closer to reaching the Sustainable Development Goals. This is exemplified in a new framework on social-ecological inclusion processes in the world of work.
New Scanning Strategy Approach for Laser Powder Bed Fusion of Nd-Fe-B Hard Magnetic Material
Authors: Pelevin Ivan A. , Terekhin Egor A. , Ozherelkov Dmitry Yu. , Tereshina Irina S. , Karpenkov Dmitry Yu. , Bochkanov Fedor Yu. , Chernyshikhin Stanislav V. , Nalivaiko Anton Yu. , Gromov Alexander A.
Published: June 2023

Volumetric cubic and cylindrical samples from MQP-S Nd-Fe-B-type material were 3D-printed using the LPBF technique. Two different scanning strategies were used: the convenient single laser exposure scanning strategy and the newly proposed double scanning strategy aimed at improving the melting process and increasing the density of the synthesized material. Samples with a relative density value higher than 95% were obtained using the new scanning strategy by reducing void volume and cracks. This was achieved by decreasing internal stresses and reducing the tendency to form and propagate cracks. The double scanning strategy of half laser power followed by full power exposure provides higher magnetic properties (both coercive force and remanence). The coercive force increases with energy input decrease, while remanence has inverse dependence.
Research on the Analysis and Prediction Model of Machining Parameters of Titanium Alloy by Abrasive Belt
Authors: Qiao Hu , Hu Sibo , Xiang Ying , Liu Shanshan , Zhang Li
Published: June 2023

As a high-performance and difficult-to-machine material for the manufacture of blades, titanium alloys are increasingly being used in high-end manufacturing industries such as aerospace and aircraft. As engineering applications become more demanding, so do the requirements for precision. However, to date, the choice of blade grinding parameters is still mainly dependent on the traditional “trial cut” and “experience” method, making the processing efficiency low and the quality of processing difficult to be guaranteed. In order to achieve the requirements of high precision and low surface roughness of the workpiece, to get rid of the status quo of relying on manual decision-making, and to achieve reasonable prediction and control of surface quality, this paper proposes to establish a theoretical prediction model for surface roughness of titanium alloy by abrasive belt grinding, and to analyze the influence of the main process parameters on surface roughness during the grinding process through experiments. A theoretical prediction model for surface roughness was developed. The experimental results show that the model has certain accuracy and reliability, and can provide guidance for the high-precision prediction of the surface roughness of ground titanium alloy blades, which has strong practical significance in engineering.
Effect of Weld Design of Transition Layer on Microstructure and Properties of Welded Joints of S32304/Q390C Composites
Authors: Feng Yulan , Wu Zhisheng , Liu Cuirong , Zhang Luxia , Wang Xin
Published: June 2023

Due to the large difference in physical and chemical properties between the substrate and the cladding material, the welding of composite materials is much more difficult than that of single materials. In our work, S32304/Q390C composite material was considered as the research object. By adjusting the welding parameters, two kinds of joint geometry were obtained, namely, the transition layer weld lower (joint A) and higher the composite material interface (joint B). We studied the influence of the transition layer weld on the microstructure and properties of welded joints. The microstructure of the transition layer weld, the distribution of elements, the Schmidt factor of the interface between the transition layer and base layer weld, and the tensile strength of the joint were evaluated. The results show that with the increase of welding heat input, the microstructure of the transition layer weld changes from austenite and skeleton ferrite to austenite and lathy ferrite and austenite and acicular ferrite, while ferrite grows towards the weld center, showing a dendritic shape and a local network structure. At the side of the base layer weld of the interface between the transition and the base layer weld, the thickness of the low-carbon-content layer increased from 100 μm to 150 μm. Iron, chromium, and nickel elements on both sides of the interface were diffused, and the thickness of the diffusion layer increased from 3 μm to 10 μm. The tensile strength values of joints A and B were 648 MPa and 668 MPa, respectively, and the Schmidt factor values were 0.446 and 0.454, respectively. Combination with the analysis of the fracture morphology showed that when the transition layer weld was higher than the interface of the composite plate, the joint had better plastic deformation ability and higher tensile strength.
Causal Effects of Blood Lipid Traits on Inflammatory Bowel Diseases: A Mendelian Randomization Study
Authors: Yao Ziqin , Jiang Feiyu , Luo Hongbin , Zhou Jiahui , Shi Wanting , Xu Shoufang , Zhang Yingying , Dai Feng , Li Xinran , Liu Zhiwei , Wang Xinhui
Published: June 2023

Inflammatory bowel diseases (IBDs), including Crohn’s disease (CD) and ulcerative colitis (UC), have become a global health problem with a rapid growth of incidence in newly industrialized countries. Observational studies have recognized associations between blood lipid traits and IBDs, but the causality still remains unclear. To determine the causal effects of blood lipid traits, including triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) on IBDs, two-sample Mendelian randomization (MR) analyses were conducted using the summary-level genome-wide association study (GWAS) statistics of blood lipid traits and IBDs. Our univariable MR using multiplicative random-effect inverse-variance weight (IVW) method identified TC (OR: 0.674; 95% CI: 0.554, 0.820; p < 0.00625) and LDL-C (OR: 0.685; 95% CI: 0.546, 0.858; p < 0.00625) as protective factors of UC. The result of our multivariable MR analysis further provided suggestive evidence of the protective effect of TC on UC risk (OR: 0.147; 95% CI: 0.025, 0.883; p < 0.05). Finally, our MR-BMA analysis prioritized TG (MIP: 0.336; θ^MACE: −0.025; PP: 0.31; θ^λ: −0.072) and HDL-C (MIP: 0.254; θ^MACE: −0.011; PP: 0.232; θ^λ: −0.04) for CD and TC (MIP: 0.721; θ^MACE: −0.257; PP: 0.648; θ^λ: −0.356) and LDL-C (MIP: 0.31; θ^MACE: −0.095; PP: 0.256; θ^λ: −0.344) for UC as the top-ranked protective factors. In conclusion, the causal effect of TC for UC prevention was robust across all of our MR approaches, which provide the first evidence that genetically determined TC is causally associated with reduced risk of UC. The finding of this study provides important insights into the metabolic regulation of IBDs and potential metabolites targeting strategies for IBDs intervention.
Anti-Alzheimer Potential of a New (+)-Pinitol Glycoside Isolated from Tamarindus indica Pulp: In Vivo and In Silico Evaluations
Authors: Mohamed Esraa M. , H. Elmaidomy Abeer , Alaaeldin Rania , Alsenani Faisal , Altemani Faisal H. , Algehainy Naseh A. , Alanazi Mohammad A , Bagalagel Alaa , Althagafi Abdulhamid , Elrehany Mahmoud A , Abdelmohsen Usama Ramadan
Published: June 2023

Tamarindus indica Linn (tamarind, F. Leguminosae) is one of the most widely consumed edible fruits in the world. Phytochemical investigation of tamarind pulp n-butanol fraction yielded one new (+)-pinitol glycoside compound 1 (25% w/w), and 1D, 2D NMR, and HRESIMS investigation were used to confirm the new compound’s structure. (+)-Pinitol glycoside showed anti-Alzheimer potential that was confirmed in prophylactic and treatment groups by decreasing time for the T-maze test; decreased TAO, brain and serum AChE, MDA, tau protein levels, and β amyloid peptide protein levels; and increasing GPX, SOD levels, and in vivo regression of the neurodegenerative features of Alzheimer’s dementia in an aluminum-intoxicated rat model. The reported molecular targets for human Alzheimer’s disease were then used in a network pharmacology investigation to examine their complex interactions and identify the key targets in the disease pathogenesis. An in silico-based analysis (molecular docking, binding free energy calculation (ΔGBinding), and molecular dynamics simulation) was performed to identify the potential targets for compound 1. The findings of this study may lead to the development of dietary supplements for the treatment of Alzheimer’s disease.
Editorial for the Special Issue on Structural Analyses and Designs for Flexible/Stretchable Electronics
Authors: Li Rui
Published: June 2023

Flexible/stretchable electronics constitute a class of prospective technology incorporating stretchable/bendable/twistable components such that unprecedented properties superior to those of conventional rigid/brittle electronics are realized over [...]
Viable Mycobacterium avium subsp. paratuberculosis Colonizes Peripheral Blood of Inflammatory Bowel Disease Patients
Authors: Estevinho Maria Manuela , Cabeda José , Santiago Mafalda , Machado Elisabete , Silva Ricardo , Duro Mary , Pita Inês , Morais Rui , Macedo Guilherme , Bull Tim J. , Magro Fernando , Sarmento Amélia
Published: June 2023

Pathobionts, particularly Mycobacterium avium subsp. paratuberculosis (MAP) and Escherichia coli isolates with adherence/invasive ability (AIEC) have been associated with inflammatory bowel disease (IBD), particularly Crohn’s disease (CD). This study aimed to evaluate the frequency of viable MAP and AIEC in a cohort of IBD patients. As such, MAP and E. coli cultures were established from faecal and blood samples (with a total n = 62 for each) of patients with CD (n = 18), ulcerative colitis (UC, n = 15), or liver cirrhosis (n = 7), as well as from healthy controls (HC, n = 22). Presumptive positive cultures were tested by polymerase chain reaction (PCR), for a positive confirmation of MAP or E. coli identity. E. coli-confirmed isolates were then tested for AIEC identity using adherence and invasion assays in the epithelial cell line of Caco-2 and survival and replication assays in the macrophage cell line of J774. MAP sub-culture and genome sequencing were also performed. MAP was more frequently cultured from the blood and faecal samples of patients with CD and cirrhosis. E. coli presumptive colonies were isolated from the faecal samples of most individuals, in contrast to what was registered for the blood samples. Additionally, from the confirmed E. coli isolates, only three had an AIEC-like phenotype (i.e., one CD patient and two UC patients). This study confirmed the association between MAP and CD; however, it did not find a strong association between the presence of AIEC and CD. It may be hypothesized that the presence of viable MAP in the bloodstream of CD patients contributes to disease reactivation.
Detection of Antagonistic Compounds Synthesized by Bacillus velezensis against Xanthomonas citri subsp. citri by Metabolome and RNA Sequencing
Authors: Rabbee Muhammad Fazle , Baek Kwang-Hyun
Published: June 2023

Biological control of plant diseases has gained attraction for controlling various bacterial diseases at a field trial stage. An isolated endophytic bacterium, Bacillus velezensis 25 (Bv-25), from Citrus species had strong antagonistic activity against Xanthomonas citri subsp. citri (Xcc), which causes citrus canker disease. When Bv-25 was incubated in Landy broth or yeast nutrient broth (YNB), the ethyl acetate extract of Landy broth exhibited higher levels of antagonistic activity against Xcc compared to that of YNB. Therefore, the antimicrobial compounds in the two ethyl acetate extracts were detected by high performance liquid chromatography–mass spectrometry. This comparison revealed an increase in production of several antimicrobial compounds, including difficidin, surfactin, fengycin, and Iturin-A or bacillomycin-D by incubation in Landy broth. RNA sequencing for the Bv-25 grown in Landy broth were performed, and the differential expressions were detected for the genes encoding the enzymes for the synthesis of antimicrobial compounds, such as bacilysin, plipastatin or fengycin, surfactin, and mycosubtilin. Combination of metabolomics analysis and RNA sequencing strongly suggests that several antagonistic compounds, especially bacilysin produced by B. velezensis, exhibit an antagonistic effect against Xcc.
Effect of Snowmelt on Groundwater Bacterial Community Composition and Potential Role of Surface Environments as Microbial Seed Bank in Two Distinct Aquifers from the Region of Quebec, Canada
Authors: Villeneuve Karine , Turcotte-Blais Valérie , Lazar Cassandre Sara
Published: June 2023

Events of groundwater recharge are associated with changes in the composition of aquifer microbial communities but also abiotic conditions. Modification in the structure of the community can be the result of different environmental condition favoring or hindering certain taxa, or due to the introduction of surface-derived taxa. Yet, in both cases, the local hydrogeochemical settings of the aquifer is likely to affect the amount of variation observed. Therefore, in our study, we used 16S rRNA gene sequencing to assess how microbial communities change in response to snowmelt and the potential connectivity between subsurface and surface microbiomes in two distinct aquifers located in the region of Vaudreuil–Soulanges (Québec, Canada). At both sites, we observed an increase in groundwater level and decrease in temperature following the onset of snow melt in March 2019. Bacterial community composition of each aquifer was significantly different (p < 0.05) between samples collected prior and after groundwater recharge. Furthermore, microbial source tracking results suggested a low contribution of surface environments to the groundwater microbiome except for in the months associated with recharge (March 2019 and April 2019). Overall, despite differences in soil permeability between both sites, the period of snow melt was followed by important changes in the composition of microbial communities from aquifers.
Origin and Evolution of Ore-Forming Fluids at the Small-Sized Gold Deposits in the Khudolaz Area, Southern Urals
Authors: Rakhimov Ildar R. , Ankusheva Natalia N. , Samigullin Aidar A. , Shanina Svetlana N.
Published: June 2023

Lode gold deposits are widespread in orogenic belts of various ages and are a valuable gold source, but their genesis remains debatable. The close relationship between native gold and quartz was considered a reason to search for acid-magmatic sources of heat and fluids (i.e., granite intrusions), while small gabbro bodies were often ignored. Six minor gold deposits associated with NE-strike faults were studied in the Khudolaz area of the South Urals (Tukan, Bilyan-Tau, Fazly-Tau, Muildy-Tamak, Alasiya-II and Isyanbet-I). It was established, for the first time, that all of the studied deposits are similar geologically but differ in mineralogical diversity of ore-bearing quartz veins, which is due to the different composition of host rocks and ore-bearing intrusions of the Khudolaz (325–329 Ma, U-Pb) and the Ulugurtau (321 ± 15 Ma, Sm-Nd) ultramafic-mafic complexes. Results of the geochemical study of quartz veins (ICP MS) and their fluid inclusions (microthermometry, gas chromatography) showed that native gold was mostly precipitated at temperatures of 230–330 °C from a low- to moderate-saline (8–12 wt.% NaCl-eq.) H2O–CO2–CH4-bearing fluid, when weakly oxidized or near-neutral conditions, were replaced by reducing ones. No significant differences between barren milky white and ore-bearing brownish quartz veins were defined, which indicates their common formation settings and an impulse pattern of vein injection. The stable pattern of the fluid salinity, along with low hydrocarbon and N2 contents, as well as a narrow range of δ18O values, indicate a prevailing magmatogenic source with a certain influence of host rocks but without the influence of meteoric waters. Based on the presented data, the studied deposits were attributed to the epizonal orogenic type. This study shows the formation of lode gold deposits is possible without the participation of granite massifs.
Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures
Authors: Sardar Tauqeer , Maqbool Mehwish , Ishtiaq Muhammad , Mazhar Muhammad Waqas , El-Sheikh Mohamed A. , Casini Ryan , Mahmoud Eman A. , Elansary Hosam O.
Published: June 2023

The challenges in the production of metabolites of medicinal potential from wild plants include low yields, slow growth rates, seasonal variations, genetic variability and regulatory as well as ethical constraints. Overcoming these challenges is of paramount significance and interdisciplinary approaches and innovative strategies are prevalently applied to optimize phytoconstituents’ production, enhance yield, biomass, ensure sustainable consistency and scalability. In this study, we investigated the effects of elicitation with yeast extract and calcium oxide nanoparticles (CaONPs) on in vitro cultures of Swertia chirata (Roxb. ex Fleming) Karsten. Specifically, we examined the effects of different concentrations of CaONPs in combination with different concentrations of yeast extract on various parameters related to callus growth, antioxidant activity, biomass and phytochemical contents. Our results showed that elicitation with yeast extract and CaONPs had significant effects on the growth and characteristics of callus cultures of S. chirata. The treatments involving yeast extract and CaONPs were found to be the most effective in increasing the contents of total flavonoid contents (TFC), total phenolic contents (TPC), amarogentin and mangiferin. These treatments also led to an improvement in the contents of total anthocyanin and alpha tocopherols. Additionally, the DPPH scavenging activity was significantly increased in the treated samples. Furthermore, the treatments involving elicitation with yeast extract and CaONPs also led to significant improvements in callus growth and characteristics. These treatments promoted callus response from an average to an excellent level and improved the color and nature of the callus from yellow to yellow-brown and greenish and from fragile to compact, respectively. The best response was observed in treatments involving 0.20 g/L yeast extract and 90 ug/L CaONPs. Overall, our findings suggest that elicitation with yeast extract and CaONPs can be a useful strategy for promoting the growth, biomass, phytochemical contents and antioxidant activity of callus cultures of S. chirata in comparison to wild plant herbal drug samples.
Bipyridine Ruthenium(II) Complexes with Halogen-Substituted Salicylates: Synthesis, Crystal Structure, and Biological Activity
Authors: Schoeller Martin , Piroš Milan , Litecká Miroslava , Koňariková Katarína , Jozefíková Flóra , Šagátová Alexandra , Zahradníková Eva , Valentová Jindra , Moncol Ján
Published: June 2023

Ruthenium complexes currently represent a perspective subject of investigation in terms of potential anticancer therapeutics. Eight novel octahedral ruthenium(II) complexes are the subject of this article. Complexes contain 2,2′-bipyridine molecules and salicylates as ligands, differing in position and type of halogen substituent. The structure of the complexes was determined via X-ray structural analysis and NMR spectroscopy. All complexes were characterized by spectral methods—FTIR, UV–Vis, ESI-MS. Complexes show sufficient stability in solutions. Therefore, their biological properties were studied. Binding ability to BSA, interaction with DNA, as well as in vitro antiproliferative effects against MCF-7 and U-118MG cell lines were investigated. Several complexes showed anticancer effects against these cell lines.
Screening of Volatile Compounds, Traditional and Modern Phytotherapy Approaches of Selected Non-Aromatic Medicinal Plants (Lamiaceae, Lamioideae) from Rtanj Mountain, Eastern Serbia
Authors: Aćimović Milica , Stanković Jeremić Jovana , Miljković Ana , Rat Milica , Lončar Biljana
Published: June 2023

Ironwort (Sideritis montana L.), mountain germander (Teucrium montanum L.), wall germander (Teucrium chamaedrys L.), and horehound (Marrubium peregrinum L.) are species widely distributed across Europe and are also found in North Africa and West Asia. Because of their wide distribution they express significant chemical diversity. For generations, these plants have been used as medical herbs for treating different aliments. The aim of this paper is to analyze volatile compounds of four selected species that belong to the subfamily Lamioideae, family Lamiaceae, and inspect scientifically proven biological activities and potential uses in modern phytotherapy in relation to traditional medicine. Therefore, in this research, we analyze the volatile compounds from this plants, obtained in laboratory by a Clevenger-type apparatus, followed by liquid–liquid extraction with hexane as the solvent. The identification of volatile compounds is conducted by GC-FID and GC-MS. Although these plants are poor in essential oil, the most abundant class of volatile components are mainly sesquiterpenes: germacrene D (22.6%) in ironwort, 7-epi-trans-sesquisabinene hydrate (15.8%) in mountain germander, germacrene D (31.8%) and trans-caryophyllene (19.7%) in wall germander, and trans-caryophyllene (32.4%) and trans-thujone (25.1%) in horehound. Furthermore, many studies show that, in addition to the essential oil, these plants contain phenols, flavonoids, diterpenes and diterpenoids, iridoids and their glycosides, coumarins, terpenes, and sterols, among other active compounds, which affect biological activities. The other goal of this study is to review the literature that describes the traditional use of these plants in folk medicine in regions where they grow spontaneously and compare them with scientifically confirmed activities. Therefore, a bibliographic search is conducted on Science Direct, PubMed, and Google Scholar to gather information related to the topic and recommend potential applications in modern phytotherapy. In conclusion, we can say that selected plants could be used as natural agents for promoting health, as a source of raw material in the food industry, and as supplements, as well as in the pharmaceutical industry for developing plant-based remedies for prevention and treatment of many diseases, especially cancer.
Enhancing Oil Recovery by Polymeric Flooding with Purple Yam and Cassava Nanoparticles
Authors: Al-Jaber Hasanain A. , Arsad Agus , Tahir Muhammad , Nuhma Mustafa Jawad , Bandyopadhyay Sulalit , Abdulmunem Abdulmunem R. , Abdul Rahman Anis Farhana , Harun Zakiah binti , Agi Augustine
Published: June 2023

Significant amounts of oil remain in the reservoir after primary and secondary operations, and to recover the remaining oil, enhanced oil recovery (EOR) can be applied as one of the feasible options remaining nowadays. In this study, new nano-polymeric materials have been prepared from purple yam and cassava starches. The yield of purple yam nanoparticles (PYNPs) was 85%, and that of cassava nanoparticles (CSNPs) was 90.53%. Synthesized materials were characterized through particle size distribution (PSA), Zeta potential distribution, Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and transmission electron microscopy (TEM). The performance of PYNPs in recovering oil was better than CSNPs, as found from the recovery experiments. Zeta potential distribution results confirmed the stability of PYNPs over CSNPs (−36.3 mV for PYNPs and −10.7 mV for CSNPs). The optimum concentration for these nanoparticles has been found from interfacial tension measurements and rheological properties, and it was 0.60 wt.% for PYNPs and 0.80 wt.% for CSNPs. A more incremental recovery (33.46%) was achieved for the polymer that contained PYNPs in comparison to the other nano-polymer (31.3%). This paves the way for a new technology for polymer flooding that may replace the conventional method, which depends on partially hydrolyzed polyacrylamide (HPAM).
The Role of Extracellular Matrix (ECM) Adhesion Motifs in Functionalised Hydrogels
Authors: Morwood Anna J. , El-Karim Ikhlas A. , Clarke Susan A. , Lundy Fionnuala T.
Published: June 2023

To create functional tissue engineering scaffolds, biomaterials should mimic the native extracellular matrix of the tissue to be regenerated. Simultaneously, the survival and functionality of stem cells should also be enhanced to promote tissue organisation and repair. Hydrogels, but in particular, peptide hydrogels, are an emerging class of biocompatible scaffolds which act as promising self-assembling biomaterials for tissue engineering and regenerative therapies, ranging from articular cartilage regeneration at joint defects, to regenerative spinal cord injury following trauma. To enhance hydrogel biocompatibility, it has become imperative to consider the native microenvironment of the site for regeneration, where the use of functionalised hydrogels with extracellular matrix adhesion motifs has become a novel, emerging theme. In this review, we will introduce hydrogels in the context of tissue engineering, provide insight into the complexity of the extracellular matrix, investigate specific adhesion motifs that have been used to generate functionalised hydrogels and outline their potential applications in a regenerative medicine setting. It is anticipated that by conducting this review, we will provide greater insight into functionalised hydrogels, which may help translate their use towards therapeutic roles.