bluuebunny/crossref_metadata_2025 huggingface dataset:
Type of cycle, temperament and childhood trauma are associated with lithium response in patients with bipolar disorders
Authors: Janiri Delfina , Simonetti Alessio , Luciano Mario , Montanari Silvia , Bernardi Evelina , Carrà Giuseppe , Fiorillo Andrea , Sani Gabriele
Published: April 2024

Background

Lithium stands as the gold standard in treating bipolar disorders (BD). Despite numerous clinical factors being associated with a favorable response to lithium, comprehensive studies examining the collective influence of clinical variables alongside psychopathological dimensions are lacking. Our study aims to enhance comprehension of lithium response in individuals with BD by integrating clinical variables with psychopathological traits and early adverse events.

Methods

We assessed 201 patients with BD for clinical characteristics, childhood trauma, temperament traits, impulsivity, and aggression. Lithium response was evaluated using the gold standard Alda scale, and predictors of lithium response were estimated through a multivariate model.

Results

On the total sample, 61 (30.3%) patients were lithium responders according to the Alda scale. Comparatively, lithium responders, in contrast to non-responders, demonstrated a higher prevalence of the mania-depression-interval (MDI) cycle, a more frequent diagnosis of BD type I, and reported an earlier age of onset. They also exhibited less lifetime substance abuse, emotional, physical, and sexual abuse, while scoring higher on hyperthymic and irritable temperament scales. In multivariate analyses, only the MDI cycle (OR,3.47; 95%CI,1.61–7.50) hyperthymic (OR,1.20; 95%CI,1.02–1.41) and irritable temperament (OR,1.28; 95%CI,1.08–1.52) persisted as significant predictors of a positive response to lithium treatment, while emotional (OR,0.87; 95%CI,0.76–0.98) and physical abuse (OR,0.83; 95%CI,0.70–0.98) were predictors of non-response.

Conclusions

In evaluating lithium response in BD, our study highlights the importance of considering clinical variables alongside temperament and childhood adversities. The assessment of hyperthymic and irritable temperament, emotional and physical abuse together with the type of cycle is of particular importance. Furthermore, our findings underscore the significance of systematically assessing the type of cycle in patients with BD through the use of life charts.
Psychometric properties of the GAD-7 (General Anxiety Disorder-7): a cross-sectional study of the Peruvian general population
Authors: Villarreal-Zegarra David , Paredes-Angeles Rubi , Mayo-Puchoc Nikol , Arenas-Minaya Enoc , Huarcaya-Victoria Jeff , Copez-Lonzoy Anthony
Published: April 2024

Background

Anxiety disorders are among the main mental health problems worldwide and are considered one of the most disabling conditions. Therefore, it is essential to have measurement tools that can be used to screen for anxiety symptoms in the general population and thus identify potential cases of people with anxiety symptoms and provide them with timely care. Our aim was to evaluate the psychometric properties of the General Anxiety Disorder-7 scale (GAD-7) in the Peruvian population.

Method

Our study was a cross-sectional study. The sample included people aged 12 to 65 years in Peru. Confirmatory factor analysis, analysis of measurement invariance, convergent validity with the Patient Health Questionnaire-9 (PHQ-9) and internal consistency analysis were performed.

Results

In total, 4431 participants were included. The one-factor model showed the best fit (CFI = 0.994; TLI = 0.991; RMSEA = 0.068; WRMR = 1.567). The GAD-7 score showed measurement invariance between men and women and between age groups (adults vs. adolescents) (ΔCFI < 0.01). The internal consistency of the one-factor model was satisfactory (ω = 0.90, α = 0.93). The relationship between depressive symptoms (PHQ-9) and anxiety symptoms (GAD-7) presented a moderate correlation (r = 0.77).

Conclusions

Our study concluded that the GAD-7 score shows evidence of validity and reliability for the one-factor model. Furthermore, because the GAD-7 score is invariant, comparisons can be made between groups (i.e., by sex and age group). Finally, we recommend the use of the GAD-7 for the general population in the Peruvian context.
Allochrony is shaped by foraging niche segregation rather than adaptation to the windscape in long-ranging seabirds
Authors: Ventura Francesco , Granadeiro José Pedro , Catry Paulo , Gjerdrum Carina , De Pascalis Federico , Viveiros Filipe , Silva Isamberto , Menezes Dilia , Paiva Vítor H , Silva Mónica C
Published: April 2024

Background

Ecological segregation allows populations to reduce competition and coexist in sympatry. Using as model organisms two closely related gadfly petrels endemic to the Madeira archipelago and breeding with a two month allochrony, we investigated how movement and foraging preferences shape ecological segregation in sympatric species. We tested the hypothesis that the breeding allochrony is underpinned by foraging niche segregation. Additionally, we investigated whether our data supported the hypothesis that allochrony is driven by species-specific adaptations to different windscapes.

Methods

We present contemporaneous tracking and stable isotopes datasets for Zino’s (Pterodroma madeira) and Desertas (Pterodroma deserta) petrels. We quantified the year-round distribution of the petrels, characterised their isotopic niches and quantified their habitat preferences using machine learning (boosted regression trees). Hidden-Markov-models were used to investigate the effect of wind on the central-place movement speed, and a simulation framework was developed to investigate whether each species breeds at times when the windscape is most favourable to sustain their trips.

Results

Despite substantial spatial overlap throughout the year, the petrels exhibited diverging isotopic niches and habitat preferences during breeding. Both species used a vast pelagic region in the North Atlantic, but targeted two different mesopelagic ecoregions and showed a preference for habitats mostly differing in sea surface temperature values. Based on our simulation framework, we found that both species would perform trips of similar speed during the other species’ breeding season.

Conclusions

The different breeding schedules between the species are underpinned by differences in foraging habitat preferences and adaptation to the local environment, rather than to the windscape. Nevertheless, the larger Desertas petrels exploited significantly windier conditions, potentially unsustainable for the smaller Zino’s petrels. Furthermore, due to larger mass and likely higher fasting endurance, Desertas petrels engaged in central-place-foraging movements that covered more ground and lasted longer than those of Zino’s petrels. Ultimately, patterns of ecological segregation in sympatric seabirds are shaped by a complex interplay between foraging and movement ecology, where morphology, foraging trip regulation and fasting endurance have an important– yet poorly understood– role.
Why people use augmented reality in heritage museums: a socio-technical perspective
Authors: Chen Yuangao , Wang Xini , Le Bin , Wang Lu
Published: April 2024

With the development of digital technologies, the use of augmented reality in the tourism sector has received increasing attention. However, the understanding of the factors influencing tourists’ post-experience intention with augmented reality devices is incomplete and there is a lack of empirical research. This study explores the impact of AR technical factors, individual factors and situational factors on users’ post-experience AR usage intention and destination revisit intention through satisfaction based on a socio-technical perspective. The survey data collected from 254 visitors who visited the Liangzhu Museum using the corresponding AR glasses were analysed, and the results showed that the novelty of the technical aspect, the technology trust of the individual aspect and the aesthetics, education and authenticity of the situational aspect influenced visitors’ AR usage intention and destination revisit intention through satisfaction. This study has an impact on future research on the application of augmented reality technology to heritage museums.
Nanoscale analysis of noble gas in solids
Authors: Bajo Ken-ichi , Yurimoto Hisayoshi
Published: April 2024

Noble gases are useful tracers for geochemistry, used to elucidate the origin and evolution of the solar system and planets. Noble gas analyses have been limited to bulk and spot analyses of solids and have yet to be developed for two- and three-dimensional imaging analysis. Recent developments in He isotope imaging using secondary neutral mass spectrometry are reviewed. The images have been fully quantified, and the spatial resolution has reached the nanoscale. The detection limit has been reduced to the level of ~ 10–3 cm3 STP g−1 (~ 1017 cm−3, ~ 1 ppma) for helium at lateral micrometer resolution. With this development, the concentration distribution of He in solids has been visualized as a map for the first time.
Impact of different models based on blood samples and images for bone marrow dosimetry after 177Lu-labeled somatostatin-receptor therapy
Authors: Vallot Delphine , Brillouet Séverine , Pondard Séléna , Vija Lavinia , Texier Jean-Sébastien , Dierickx Lawrence , Courbon Frédéric
Published: April 2024

Background

Peptide receptor radionuclide therapy with 177Lu-DOTATATE is a recognized option for treating neuroendocrine tumors and has few toxicities, except for the kidneys and bone marrow. The bone marrow dose is generally derived from a SPECT/CT image-based method with four timepoints or from a blood-based method with up to 9 timepoints, but there is still no reference method. This retrospective single-center study on the same cohort of patients compared the calculated bone marrow dose administered with both methods using mono, bi- or tri-exponential models. For the image-based method, the dose was estimated using Planetdose© software. Pearson correlation coefficients were calculated. We also studied the impact of late timepoints for both methods.

Results

The bone marrow dose was calculated for 131 treatments with the blood-based method and for 17 with the image-based method. In the former, the median absorbed dose was 15.3, 20.5 and 28.3 mGy/GBq with the mono-, bi- and tri-exponential model, respectively. With the image-based method, the median absorbed dose was 63.9, 41.9 and 60.8 with the mono-, bi- and tri-exponential model, respectively. Blood samples after 24h post-injection did not evidence any change in the absorbed bone marrow dose with the bi-exponential model. On the contrary, the 6-day post-injection timepoint was more informative with the image-based model.

Conclusion

This study confirms that the estimated bone marrow dose is significantly lower with the blood-based method than with the image-based method. The blood-based method with a bi-exponential model proved particularly useful, without the need for blood samples after 24h post-injection. Nevertheless, this blood-based method is based on an assumption that needs to be more validated. The important difference between the two methods does not allow to determine the optimal one to estimate the true absorbed dose and further studies are necessary to compare with biological effects.
Comparing the Impacts of Testosterone and Exercise on Lean Body Mass, Strength and Aerobic Fitness in Aging Men
Authors: Green Daniel J. , Chasland Lauren C. , Yeap Bu B. , Naylor Louise H.
Published: April 2024

Background

Based on the largely untested premise that it is a restorative hormone that may reverse the detrimental impacts of aging, prescription of testosterone (T) has increased in recent decades despite no new clinical indications. It is apparent that middle-aged and older men with low-normal serum T levels are considering T supplementation as an anti-aging strategy. At the same time, there is evidence that physical activity (PA) is at historical lows in the Western world. In this review, we compare the impacts of T treatment aimed at achieving physiological T concentrations in middle-aged and older men, alongside the impacts of ecologically relevant forms of exercise training. The independent, and possible combined, effects of T and exercise therapy on physiological outcomes such as aerobic fitness, body composition and muscular strength are addressed.

Main Body

Our findings suggest that both T treatment and exercise improve lean body mass in healthy older men. If improvement in lean body mass is the primary aim, then T treatment could be considered, and the combination of T and exercise may be more beneficial than either in isolation. In terms of muscle strength in older age, an exercise program is likely to be more beneficial than T treatment (where the dose is aimed at achieving physiological concentrations), and the addition of such T treatment does not provide further benefit beyond that of exercise alone. For aerobic fitness, T at doses aimed at achieving physiological concentrations has relatively modest impacts, particularly in comparison to exercise training, and there is limited evidence as to additive effects. Whilst higher doses of T, particularly by intramuscular injection, may have larger impacts on lean body mass and strength, this must be balanced against potential risks.

Conclusion

Knowing the impacts of T treatment and exercise on variables such as body composition, strength and aerobic fitness extends our understanding of the relative benefits of physiological and pharmacological interventions in aging men. Our review suggests that T has impacts on strength, body composition and aerobic fitness outcomes that are dependent upon dose, route of administration, and formulation. T treatment aimed at achieving physiological T concentrations in middle-aged and older men can improve lean body mass, whilst exercise training enhances lean body mass, aerobic fitness and strength. Men who are physically able to exercise safely should be encouraged to do so, not only in terms of building lean body mass, strength and aerobic fitness, but for the myriad health benefits that exercise training confers.
The game of lies by stock investors in social media: a study based on city lockdowns in China
Authors: Liu Qing , Son Hosung , Lee Woon-Seek
Published: April 2024

The potential hypotheses for finance research based on social media sentiment revolve around the reliability of investor sentiment expressed on social media and the causal relationship between financial markets and this sentiment. The central hypothesis we focus on is derived from the "lie game" played by investors on social media. This study is the first to explore three states of this lie game in the context of the Chinese stock market: the "equilibrium state", the "confusion state", and the "subversion state". Our findings indicate that the "equilibrium" state is the typical state of the lie game, where increased investor sentiment results in more positive market behavior, and higher stock prices lead to increased investor sentiment. We also examine the effect of significant social events, such as the "lockdown in Wuhan" and the "lockdown in Shanghai", on the lie game's outcome. The successful lockdown in Wuhan and the public's opposition to the politicization of COVID-19 reinforced the "equilibrium" state of the game. However, the Shanghai lockdown's failure to promptly halt the spread of COVID-19 led to the intertwining of the economy and COVID-19 in public discourse, shifting the lie game's outcome from an "equilibrium state" to a "subversive state". We emphasize that the "confusion state" and "subversion state" outcomes of the lie game are concerning, and managing public opinion and the externalization of domestic conflicts can help reduce this risk. This study offers a fresh perspective on the traditional issues of investor sentiment reliability and the causal relationship between investor sentiment and stock markets.
Acceptability of automatic referrals to supportive and palliative care by patients living with advanced lung cancer: qualitative interviews and a co-design process
Authors: Ahmed Sadia , Simon Jessica , Biondo Patricia , Slobogian Vanessa , Shirt Lisa , King Seema , Paolucci Alessandra , Pabani Aliyah , Hao Desiree , Bossio Emi , Cross Ralph , Monds Tim , Nieuwenhuis Jane , Sinnarajah Aynharan
Published: April 2024

Purpose

Timely access to supportive and palliative care (PC) remains a challenge. A proposed solution is to trigger an automatic referral process to PC by pre-determined clinical criteria. This study sought to co-design with patients and providers an automatic PC referral process for patients newly diagnosed with stage IV lung cancer.

Methods

In Step 1 of this work, nine one on one phone interviews were conducted with advanced lung cancer patients on their perspectives on the acceptability of phone contact by a specialist PC provider triggered by an automatic referral process. Interviews were thematically analysed. Step 2: Patient advisors, healthcare providers (oncologists, nurses from oncology and PC, clinical social worker, psychologist), and researchers were invited to join a working group to provide input on the development and implementation of the automatic referral process. The group met biweekly (virtually) over the course of six months.

Results

From interviews, the concept of an automatic referral process was perceived to be acceptable and beneficial for patients. Participants emphasized the need for timely support, access to peer and community resources. Using these findings, the co-design working group identified eligibility criteria for identifying newly diagnosed stage IV lung cancer patients using the cancer centre electronic health record, co-developed a telephone script for specialist PC providers, handouts on supportive care, and interview and survey guides for evaluating the implemented automatic process.

Conclusion

A co-design process ensures stakeholders are involved in program development and implementation from the very beginning, to make outputs relevant and acceptable for stage IV lung cancer patients.
Hippo pathway in cell–cell communication: emerging roles in development and regeneration
Authors: Nita Akihiro , Moroishi Toshiro
Published: April 2024

The Hippo pathway is a central regulator of tissue growth that has been widely studied in mammalian organ development, regeneration, and cancer biology. Although previous studies have convincingly revealed its cell-autonomous functions in controlling cell fate, such as cell proliferation, survival, and differentiation, accumulating evidence in recent years has revealed its non-cell-autonomous functions. This pathway regulates cell–cell communication through direct interactions, soluble factors, extracellular vesicles, and the extracellular matrix, providing a range of options for controlling diverse biological processes. Consequently, the Hippo pathway not only dictates the fate of individual cells but also triggers multicellular responses involving both tissue-resident cells and infiltrating immune cells. Here, we have highlighted the recent understanding of the molecular mechanisms by which the Hippo pathway controls cell–cell communication and discuss its importance in tissue homeostasis, especially in development and regeneration.
Measuring refugees’ capabilities: translation, adaptation, and valuation of the OxCAP-MH into Juba Arabic for use among South Sudanese male refugees in Uganda
Authors: van der Boor C.F. , Taban D. , Ismail K. , Simon J. , Roberts B. , Fuhr D. , Tol W.A. , Greco G.
Published: April 2024

Background

Forcibly displaced populations are highly vulnerable to psychosocial distress and mental disorders, including alcohol misuse. In an ongoing trial that seeks to develop a transdiagnostic intervention addressing psychological distress and alcohol use disorders among conflict-affected populations, we will carry out a cost-effectiveness evaluation using a capability-based Oxford Capabilities Mental Health (OxCAP-MH) measure. The OxCAP-MH is a 16-item questionnaire developed from the Capability Approach, that covers multiple domains of functioning and welfare. The aim of the current paper is to present the results of the translation, cultural adaptation and valuation of the OxCAP-MH into Juba Arabic for South Sudanese refugees living in Uganda.

We adhered to the official Translation and Linguistic Validation process of the OxCAP-MH. To carry out the translation, the Concept Elaboration document, official English version of the OxCAP-MH, and the Back-Translation Review Template were used. Four independent translators were used for forward and back translations. The reconciled translated version was then piloted in two focus group discussions (N = 16) in Rhino refugee settlement. A most important to least important valuation of the sixteen capability domains covered in the OxCAP-MH was also done.

Results

The Juba Arabic version of the OxCAP-MH was finalized following a systematic iterative process. The content of the Juba Arabic version remained unchanged, but key concepts were adapted to ensure cultural acceptability, feasibility, and comprehension of the measure in the local context of Rhino refugee settlement. Most participants had low levels of literacy and required support with filling in the tool. Participants suggested an additional capability that is currently not reflected in the OxCAP-MH, namely access to food. Furthermore, discussions around the valuation exercise of the sixteen domains led to two separate importance scales, which showed relevant differences.

Conclusions

In this context, the OxCAP-MH was considered culturally acceptable. The valuation exercise proved cognitively demanding. Participants voiced confusion over how to answer the questions on the OxCAP-MH instrument due to low levels of literacy. These concerns invite consideration for future research to consider how measures such as the OxCAP-MH can be made more accessible to individuals with low literacy rates in resource poor settings.
The (missing) social aspect of the circular economy: a review of social scientific articles
Authors: Zavos Stylianos , Lehtokunnas Taru , Pyyhtinen Olli
Published: April 2024

Academic research on the Circular Economy has been proliferating at an unprecedented pace during the last decade. However, scholarly work on the topic is dominated by a focus on hard scientific, technical and corporate/business management approaches, leaving the social sciences underrepresented in the relevant literature. This review article covers the current Anglophone social scientific research on the circular economy with special attention to waste. A total of 161 works aligning with the scope of this article were examined. These works were analysed in light of two questions: the reviewed studies’ knowledge interest and the dimension(s) of the CE they gave emphasis to. In result, the articles were charted along two axes: Instrumental/Technical (Quadrant I), Analytical/Technical (Quadrant II), Instrumental/Social (Quadrant III), and Analytical/Social (Quadrant IV). The findings of this review article demonstrate a strong thematic interest related to the circular economy in global, major issues of governance; transition and implementation; consumption and consumer behaviour; as well as the associated logic, concepts and definitions. A weaker thematic interest appeared in relation to the cultural, political and ethical dimensions of the circular economy, while critical engagements with and contestations of the model remain fairly rare. Moreover, the analysis revealed the relative absence of detailed empirical scholarship on the more-than-human relations and the micro-level, local everyday practices through which the circular economy becomes actualised. This review calls for the proliferation of such works currently situated in the margins of the circular economy literature. However, as is finally proposed, a balanced mapping of a circular economy transition would require an approach that would problematise levels, scales and dichotomies like ‘global’ and ‘local’ as categorical givens.
Four sugarcane ScDIR genes contribute to lignin biosynthesis and disease resistance to Sporisorium scitamineum
Authors: Li Xiufang , Liu Zongling , Wu Haoming , Yu Zhuoxin , Meng Jiaorong , Zhao Haiyun , Deng Xingli , Su Yizu , Chen Baoshan , Li Ru
Published: April 2024

Sugarcane (Saccharum spp.) is a major sucrose and bioenergy crop in the world. The fungal pathogen Sporisorium scitamineum causes sugarcane smut, a devastating disease that destroys stalks and reduces sugar content in sugarcane. This disease can be controlled most effectively by applying smut-resistant sugarcane varieties. Previous studies have shown that Dirigent (DIR) genes are involved in the synthesis of the lignin precursor pinoresinol, which plays a crucial role in plant resistance to biotic stresses. However, the immune response of the DIR homologs in sugarcane (ScDIR) has not been reported yet. In this study, we found that the lignin content of smut-resistant sugarcane varieties (ZZ1, ZZ6, and ZZ9) was significantly higher than that of smut-susceptible varieties (GT42, ROC22, and FN41), and the lignin content of sugarcane increased after smut infection. The smut-resistant and smut-susceptible clones derived from the same genetic population (ROC25 × YZ89-7) showed similar patterns. Quantitative real-time PCR assays revealed that among the 64 DIR genes in sugarcane, ScDIR5, ScDIR7, ScDIR11, and ScDIR40 showed elevated expression after S. scitamineum infection. In vitro coupling reactions showed that the four corresponding ScDIR proteins could mediate the coupling of coniferyl alcohol and its conversion into the lignin precursor pinoresinol. Overexpression of the four ScDIR genes in Nicotiana benthamiana enhanced disease resistance to the fungal pathogens Sclerotium rolfsii, Rhizoctonia solani, and Botrytis cinerea. Moreover, transgenic sugarcane overexpressing these ScDIR genes showed enhanced resistance to smut disease. Taken together, our findings provide evidence that sugarcane ScDIR genes can improve the resistance of plants to fungal pathogens and highlight their potentials in sugarcane breeding for disease resistance.
Cyberchondria and smartphone addiction: A correlation survey among undergraduate medical students in Egypt
Authors: El-Zoghby Safaa M. , Zaghloul Nancy M. , Tawfik Ayat M. , Elsherbiny Noura M. , Shehata Shaimaa A. , Soltan Enayat M.
Published: April 2024

Background

There is an increase in smartphone usage globally. Cyberchondria (CYB) is characterized by excessive Internet searches for health information. Smartphone addiction is constantly rising among medical students together with CYB as a collateral emerging risk, yet there is limited research available on the topic.

Methods

This cross-sectional study explores the rising phenomenon of smartphone addiction and its potential role in CYB among medical students in seven Egyptian universities. A total of 1435 medical students participated in completing online questionnaires that assessed smartphone addiction and CYB using the Smartphone Addiction Scale-Short Version (SAS-SV) and Cyberchondria Severity Scale (CSS-12). Multivariable regression analysis was applied to assess predictors of smartphone addiction and CYB.

Results

Based on the used scales, 57.6% of students were suffering from smartphone addiction, and 85.8% exhibited a moderate degree of CYB. There was a significant positive correlation between smartphone addiction scores and CYB (p < 0.05). The multivariable regression models revealed that four factors including using Facebook, using WhatsApp, increasing time spent on the Internet per day, and a higher CYB score increase the probability of smartphone addiction. The factors that increase the probability of CYB are using Facebook, an increase in the frequency of Internet searches, a higher degree of worry about one’s health or a family member’s health, and being a smartphone addict.

Conclusion

Smartphone addiction among undergraduate medical students is prevalent. Social media use, time spent online, and smartphone addiction were linked with the risk of CYB. Regular physical activity decreases the probability of smartphone addiction. Awareness programs and increased mental and physical activities are required to help reduce smartphone addiction among youth.
Aerodynamic tunnel for tests of turbine annular cascades
Authors: Lapuzin Alexandr , Subotovich Valery , Yudin Yuriy , Malymon Ivan
Published: April 2024

Aerodynamic test rigs are necessary for experimental testing of turbomachines and investigation of possible ways to improve machine performance. Existing installations give higher losses and do not work efficiently at off-design operating modes. An operating part with an adjustable radial diffuser was designed in order to determine the characteristics of turbine annular cascades. Experimental studies and computational verifications showed satisfactory results at various operating modes. The regulated backwall of the radial diffuser ensured supersonic velocity values behind the cascade and overall stable operation in a wide range of Mach numbers up to 1.3 when using compressors of comparatively small capacity. The optimum positions of the regulated backwall were determined, which provided a deep vacuum behind the cascade, as well as 1.5 times Mach number increase compared to the turbine cascade without a diffuser. Changing the inlet channel geometry at supersonic modes leads to an increase in the diffuser efficiency. Additionally, it was determined that the use of the turbine vane cascade in the test rig flow path is not necessary during calculation studies, but instead an axisymmetric vaneless converging area can be applied and give satisfactory results as well as reduce the time spent on calculations. The computational model can be used to optimize the design of an aerodynamic tunnel outlet area.
Manufacturing of isocyanate-based oligomeric dyes with high coloring capabilities: synthesis and application in the dyeing of organic chrome-free leather
Authors: Ding Wei , Guo Song , Liu Haiteng , Pang Xiaoyan , Ding Zhiwen , Remón Javier
Published: April 2024

Leather dyeing is a critical step in leather manufacturing, as it is responsible for providing leather products with an eye-catching visual aspect and adequate quality properties to meet customers' expectations. This step is becoming more and more challenging as the leather industry advances hand in hand with new environmentally friendly policies and regulations to achieve a safer and healthier planet by replacing the highly polluting Cr-based leather tanning technology with greener alternatives. As a result, achieving high-performance dyeing of organic chrome-free leather is one of the bottlenecks for the sustainable development of the leather industry. Herein, we propose a novel strategy to fabricate an isocyanate-based oligomeric dye (IBD) with high coloring capabilities (component content higher than 62.8%) based on toluene 2,4-diisocyanate and reactive red dye 180. This material has been tested for the dyeing of biomass-derived aldehyde (BDA)-tanned leather with excellent outcomes. The experimental results showed that the crust leather dyed with our novel IBD dyeing agent had higher color fastness and better fullness than the leather dyed with conventional anionic (CAD) or reactive red 180 (RRD-180) dyes. These excellent and promising results open new avenues in manufacturing high-performance organic Cr-free leather products and help to ensure the sustainable transition of the leather industry from Cr-based leather tanning to more sustainable alternatives, maintaining the final quality of the leather products.

Graphical abstract
Thresholds in PROMIS Scores Anchored to Subsequent Unscheduled Health Service Use Among People Diagnosed With Cancer
Authors: Sikorskii Alla , Tam Samantha , Given Barbara , Given Charles W. , Adjei Boakye Eric , Zatirka Theresa , Nair Mrudula , Su Wan-Ting K. , Jogunoori Smitha , Watson Peter , Movsas Benjamin , Chang Steven
Published: April 2024

PURPOSE

To establish thresholds in the Patient-Reported Outcomes Measurement Information System (PROMIS) pain interference, physical function, fatigue, and depression scores on the basis of their association with subsequent use of the emergency department (ED) or urgent care by people diagnosed with cancer.

METHODS

Retrospective data from 952 people seen at Henry Ford Cancer and insured through the Health Alliance Plan were analyzed using generalized linear mixed-effects models. The log odds of ED or urgent care use during 14 or 30 days after each patient-reported outcome (PRO) assessment were related to PRO scores, while adjusting for comorbidity, sociodemographic, and tumor characteristics.

RESULTS

Pain interference and physical function were associated with subsequent ED or urgent care visits, but fatigue and depression were not, and the results for 14- and 30-day visits were similar. Thresholds anchored in the likelihood of these visits differed according to cancer stage. For people with advanced cancer, a pain interference score of 60 or higher (odds ratio [OR] 3.75, [95% CI, 1.53 to 7.87]) and a physical function score lower than 40 (OR 2.94, [95% CI, 1.22 to 7.06]) produced the largest ORs with narrowest CIs for 30-day visits. For people with nonadvanced cancer, the thresholds of 65 for pain interference (OR 2.64, [95% CI, 1.40 to 5.01]) and 35 for physical function (OR 1.87, [95% CI, 1.01 to 3.45]) produced largest ORs with narrowest CIs for 30-day visits.

CONCLUSION

These anchor-based thresholds in PROMIS scores can inform clinicians' actions with the goal of preventing ED or urgent care visits.
Solving Missing Heritability in Patients With Familial Adenomatous Polyposis With DNA-RNA Paired Testing
Authors: Young Colin C. , Horton Carolyn , Grzybowski Jessica , Abualkheir Nelly , Ramirez Castano Jesus , Molparia Bhuvan , Karam Rachid , Chao Elizabeth , Richardson Marcy E.
Published: April 2024

PURPOSE

Patients with germline pathogenic variants (PVs) in
APC
develop tens (attenuated familial adenomatous polyposis [AFAP]) to innumerable (classic FAP) adenomatous polyps in their colon and are at significantly increased lifetime risk of colorectal cancer. Up to 10% of FAP and up to 50% of patients with AFAP who have undergone DNA-only multigene panel testing (MGPT) do not have an identified PV in
APC
. We seek to demonstrate how the addition of RNA sequencing run concurrently with DNA can improve detection of germline PVs in individuals with a clinical presentation of AFAP/FAP.

METHODS

We performed a retrospective query of individuals tested with paired DNA-RNA MGPT from 2021 to 2022 at a single laboratory and included those with a novel
APC
PV located in intronic regions infrequently covered by MGPT, a personal history of polyposis, and family medical history provided. All clinical data were deidentified in this institutional review board-exempt study.

RESULTS

Three novel
APC
variants were identified in six families and were shown to cause aberrant splicing because of the creation of a deep intronic cryptic splice site that leads to an RNA transcript subject nonsense-mediated decay. Several carriers had previously undergone DNA-only genetic testing and had received a negative result.

CONCLUSION

Here, we describe how paired DNA-RNA MGPT can be used to solve missing heritability in FAP families, which can have important implications in family planning and treatment decisions for patients and their families.
Concordance in Molecular Tumor Board Case Reviews in the ASCO TAPUR Study
Authors: Perez Jacqueline K. , Kleber Jeanene , Rothe Michael , Mangat Pam , Garrett-Mayer Elizabeth , Schilsky Richard L.
Published: April 2024

PURPOSE

With the advent of precision medicine, molecular tumor boards (MTBs) were established to interpret genomic results and guide decision making for targeted therapy in oncology patients. There are currently no universal guidelines for how MTBs should operate and thus variance can be seen depending on which MTB is reviewing the case. This study assesses the concordance of MTB recommendations when a participant case is reviewed by two different MTBs, establishes potential reasons for discordance, and advocates for the establishment of standard MTB operating guidelines.

PATIENTS AND METHODS

Participants with advanced cancer, who had exhausted all standard treatment options were screened for the Targeted Agent and Profiling Utilization Registry (TAPUR) Study. Cases were submitted for MTB review if the treatment proposal was outside the protocol genomic matching rules, or if multiple treatment options were identified. Of the 306 cases submitted for review by the TAPUR MTB from 2016 to 2018, 107 were randomly selected for secondary review by a different MTB group. Recommendations from the original review were not disclosed. Concordance between MTB group recommendations was assessed. Concordance was defined as agreement between MTB reviews on the genomic alteration and study drug match proposed by the clinical site. Thematic qualitative analysis was conducted for the discordant cases to assess reasons for discordance.

RESULTS

Complete or partial concordance was observed in 79% of cases (95% CI, 70 to 86; one-sided P = .25). Most discordant analyses were due to disagreements on the strength of evidence regarding efficacy of the proposed treatment (32%).

CONCLUSION

When presented with identical participant cases, different MTB review groups make the same or similar treatment recommendations approximately 80% of the time.
Hormonal Basis of Biological Sex Differences in Human Athletic Performance
Authors: Senefeld Jonathon W , Hunter Sandra K
Published: April 2024

Biological sex is a primary determinant of athletic human performance involving strength, power, speed, and aerobic endurance and is more predictive of athletic performance than gender. This perspective article highlights 3 key medical and physiological insights related to recent evolving research into the sex differences in human physical performance: (1) sex and gender are not the same; (2) males and females exhibit profound differences in physical performance with males outperforming females in events and sports involving strength, power, speed, and aerobic endurance; (3) endogenous testosterone underpins sex differences in human physical performance with questions remaining on the roles of minipuberty in the sex differences in performance in prepubescent youth and the presence of the Y chromosome (SRY gene expression) in males, on athletic performance across all ages. Last, females are underrepresented as participants in biomedical research, which has led to a historical dearth of information on the mechanisms for sex differences in human physical performance and the capabilities of the female body. Collectively, greater effort and resources are needed to address the hormonal mechanisms for biological sex differences in human athletic performance before and after puberty.
The Shot CH1 domain recognises a distinct form of F-actin during Drosophila oocyte determination
Authors: Nashchekin Dmitry , Squires Iolo , Prokop Andreas , St Johnston Daniel
Published: April 2024

In Drosophila, only one cell in a multicellular female germline cyst is specified as an oocyte and a similar process occurs in mammals. The symmetry-breaking cue for oocyte selection is provided by the fusome, a tubular structure connecting all cells in the cyst. The Drosophila spectraplakin Shot localises to the fusome and translates its asymmetry into a polarised microtubule network that is essential for oocyte specification, but how Shot recognises the fusome is unclear. Here, we demonstrate that the actin-binding domain (ABD) of Shot is necessary and sufficient to localise Shot to the fusome and mediates Shot function in oocyte specification together with the microtubule-binding domains. The calponin homology domain 1 of the Shot ABD recognises fusomal F-actin and requires calponin homology domain 2 to distinguish it from other forms of F-actin in the cyst. By contrast, the ABDs of utrophin, Fimbrin, Filamin, Lifeact and F-tractin do not recognise fusomal F-actin. We therefore propose that Shot propagates fusome asymmetry by recognising a specific conformational state of F-actin on the fusome.
A geometrical model of cell fate specification in the mouse blastocyst
Authors: Raju Archishman , Siggia Eric D.
Published: April 2024

The lineage decision that generates the epiblast and primitive endoderm from the inner cell mass (ICM) is a paradigm for cell fate specification. Recent mathematics has formalized Waddington's landscape metaphor and proven that lineage decisions in detailed gene network models must conform to a small list of low-dimensional stereotypic changes called bifurcations. The most plausible bifurcation for the ICM is the so-called heteroclinic flip that we define and elaborate here. Our re-analysis of recent data suggests that there is sufficient cell movement in the ICM so the FGF signal, which drives the lineage decision, can be treated as spatially uniform. We thus extend the bifurcation model for a single cell to the entire ICM by means of a self-consistently defined time-dependent FGF signal. This model is consistent with available data and we propose additional dynamic experiments to test it further. This demonstrates that simplified, quantitative and intuitively transparent descriptions are possible when attention is shifted from specific genes to lineages. The flip bifurcation is a very plausible model for any situation where the embryo needs control over the relative proportions of two fates by a morphogen feedback.
DLC1 promotes mechanotransductive feedback for YAP via RhoGAP-mediated focal adhesion turnover
Authors: Hooglugt Aukie , van der Stoel Miesje M. , Shapeti Apeksha , Neep Beau F. , de Haan Annett , van Oosterwyck Hans , Boon Reinier A. , Huveneers Stephan
Published: April 2024

Angiogenesis is a tightly controlled dynamic process demanding a delicate equilibrium between pro-angiogenic signals and factors that promote vascular stability. The spatiotemporal activation of the transcriptional co-factors YAP (herein referring to YAP1) and TAZ (also known WWTR1), collectively denoted YAP/TAZ, is crucial to allow for efficient collective endothelial migration in angiogenesis. The focal adhesion protein deleted-in-liver-cancer-1 (DLC1) was recently described as a transcriptional downstream target of YAP/TAZ in endothelial cells. In this study, we uncover a negative feedback loop between DLC1 expression and YAP activity during collective migration and sprouting angiogenesis. In particular, our study demonstrates that signaling via the RhoGAP domain of DLC1 reduces nuclear localization of YAP and its transcriptional activity. Moreover, the RhoGAP activity of DLC1 is essential for YAP-mediated cellular processes, including the regulation of focal adhesion turnover, traction forces, and sprouting angiogenesis. We show that DLC1 restricts intracellular cytoskeletal tension by inhibiting Rho signaling at the basal adhesion plane, consequently reducing nuclear YAP localization. Collectively, these findings underscore the significance of DLC1 expression levels and its function in mitigating intracellular tension as a pivotal mechanotransductive feedback mechanism that finely tunes YAP activity throughout the process of sprouting angiogenesis.
Correlation of age with the size of subcortical nuclei of the brain and its implication in degenerative disease: A magnetic resonance imaging study
Authors: Dhamija Aditij , Andrade Lydia S. , K. Prakashini , Gupta Chandni
Published: April 2024

Background Aging is a non-modifiable risk factor for neurodegenerative disease. It is well established that the brain undergoes physiological atrophy with age. So, this study was conducted to analyse the correlation between the age of the person and the size of the various subcortical nuclei of the brain and whether these measurements can serve as a useful indicator for physiological atrophy leading to degenerative disease in clinical practice. Methods A total of 600 MRI scans from healthy individuals were examined and the measurements of subcortical nuclei were taken and subsequently analysed. Results A statistically significant difference between the genders was observed in the sizes of the axial diameters of caudate nucleus, putamen and globus pallidus. Caudate nucleus transverse diameter showed a moderate negative correlation with age in males. Globus pallidus axial diameter with age showed weak positive correlation for males. Globus pallidus transverse diameter showed weak positive correlation with age for both males and females, but it was stronger for males compared to females. Conclusions These results will help neurologists and neurosurgeons in analysing various early degenerative diseases and treat them accordingly.
pH-Induced structural changes in xylanase GH11 from Thermoanaerobacterium saccharolyticum
Authors: Nam Ki Hyun
Published: April 2024

Background Glycosyl hydrolase 11 (GH11) xylanase is utilized in various in industrial applications such as baking, fruit juice production, pulp processing, and animal feed. Thermophilic GH11 from Thermoanaerobacterium saccharolyticum (TsaGH11) exhibits maximum activity at acid pH with high catalytic efficiency toward beechwood xylan. TsaGH11 activity is pH dependent, exhibiting relative low hydrolase activity at basic pH. However, the effect of a basic pH environment on the structure of TsaGH11 correlated with enzyme activity remains unknown. To understand pH-dependent activity changes, the crystal structure of TsaGH11 at basic pH was determined and compared with that of TsaGH11 at acid pH. Methods TsaGH11 was crystallized at basic pH of 8.5, and the crystal structure was determined at 1.95 Å resolution. The structure, flexibility, and water molecules of TsaGH11 at pH 8.5 and pH 4.3 were compared. Results The open and closed conformations of TsaGH11 at pH 8.5 are reported. Subtle movements of the side chains of amino acids involved in the substrate-binding cleft and catalytic residues were observed. The overall temperature factor of TsaGH11 at pH 8.5 was higher than that at pH 4.6. The position of water molecules near the catalytic residues in TsaGH11 exhibited variations in different pH environments. Conclusions The structural comparison of TsaGH11 at basic and acidic pH offers valuable insights into the pH-dependent functionality of TsaGH11, enhancing our understanding of these structural alterations.