bluuebunny/crossref_metadata_2025 huggingface dataset:
The Relationship Between Intolerance of Uncertainty and Employment Anxiety of Graduates During COVID-19: The Moderating Role of Career Planning
Authors: Chen Li , Zeng Shuyu
Published: October 2021

COVID-19, which is characterized by uncertainty, makes the employment anxiety of college graduates in the period of career change more and more intense. How to deal with this challenge is particularly important. The present study took career planning as a moderating variable to explore the relationship between intolerance of uncertainty (IU) and employment anxiety, as well as the role of career planning. In this quantitative study, the data of 563 college graduates from different schools were collected by using the Intolerance of Uncertainty Scale-12, Vocational Selection Anxiety Questionnaire of University Graduate, and Career Planning Scale. The final effective sample size was 550 (the overall recovery rate was 97.7%). The results of correlation analysis showed that there was a significant positive correlation between IU and employment anxiety, while there was a significant negative correlation between career planning and IU, as well as the relationship between career planning and employment anxiety. The results of hierarchical regression analysis showed that IU significantly positively predicted the employment anxiety of graduates, and career planning moderated the relationship between IU and employment anxiety. These findings suggested that maintaining a sense of career planning can help college graduates get through smoothly in the face of uncertainty of COVID-19.
The Moderating Effect of Attention-Deficit Hyperactivity Disorder Symptoms on the Relationship Between Procrastination and Internalizing Symptoms in the General Adult Population
Authors: Oguchi Mana , Takahashi Toru , Nitta Yusuke , Kumano Hiroaki
Published: October 2021

Background: The symptoms of attention-deficit hyperactivity disorder (ADHD) are known to exacerbate the effect of cognitive-behavioral impairments on emotional burden. Although adults with ADHD frequently experience procrastination and internalizing symptoms such as depression and anxiety, few studies have examined whether the association between procrastination and internalizing symptoms differs by ADHD symptoms.

Objective: This study aimed to examine the moderating effect of ADHD symptoms on the association between procrastination and internalizing symptoms.

Method: A cross-sectional survey was conducted among 470 adults (mean age=26.57, standard deviation=2.93) using self-reported questionnaires: Adult ADHD Self-Report Scale, General Procrastination Scales, Patient Health Questionnaire-9, and State–Trait Anxiety Inventory.

Conclusion: Participants with more substantial ADHD symptoms experienced more procrastination and internalizing symptoms than those with the less substantial ADHD symptoms. Therefore, procrastination constitutes the treatment target for those suffering from ADHD and comorbid internalizing symptoms. Alternatively, there was no enhancing effect of ADHD symptoms on the association between procrastination and internalizing symptoms. It is necessary to examine more precise and valid hypotheses and underlying mechanisms of procrastination in high and low ADHD symptom groups.
The Behavioral Sequelae of Cannabis Use in Healthy People: A Systematic Review
Authors: Sorkhou Maryam , Bedder Rachel H. , George Tony P.
Published: October 2021

Background: Cannabis is known to have a broad range of effects on behavior, including experiencing a “high” and tranquility/relaxation. However, there are several adverse behavioral sequalae that can arise from cannabis use, depending on frequency of use, potency (e.g., THC content), age of onset, and cumulative exposure. This systematic review examined evidence for cannabis-related adverse behavioral sequalae in otherwise healthy human subjects.

Methods: Following PRISMA guidelines, we conducted a systematic review of cross-sectional and longitudinal studies from 1990 to 2020 that identified cannabis-related adverse behavioral outcomes in subjects without psychiatric and medical co-morbidities from PubMed and PsychInfo searches. Key search terms included “cannabis” OR “tetrahydrocannabinol” OR “cannabidiol” OR “marijuana” AND “anxiety” OR “depression” OR “psychosis” OR “schizophrenia” “OR “IQ” OR “memory” OR “attention” OR “impulsivity” OR “cognition” OR “education” OR “occupation”.

Results: Our search detected a total of 2,870 studies, from which we extracted 124 relevant studies from the literature on cannabis effects in the non-clinical population. Effects of cannabis on several behavioral sequelae including cognition, motivation, impulsivity, mood, anxiety, psychosis intelligence, and psychosocial functioning were identified. The preponderance of the evidence suggests that frequency of cannabis use, THC (but not CBD) content, age of onset, and cumulative cannabis exposure can all contribute to these adverse outcomes in individuals without a pre-existing medical condition or psychiatric disorder. The strongest evidence for the negative effects of cannabis are for psychosis and psychosocial functioning.

Conclusions: Although more research is needed to determine risk factors for development of adverse behavioral sequelae of cannabis use, these findings underline the importance of understanding vulnerability to the adverse effects of cannabis, which has implications for prevention and treatment of problematic cannabis use.
SARS-CoV-2 Seroprevalence and Profiles Among Convalescents in Sichuan Province, China
Authors: Zhou Lijun , Li Cheng , Yang Huiping , Yuan Heng , Pan Ming , Cheng Xiuwei , Xiao Chongkun , Su Xiaoyan , Zhu Yuanfang , Xu Jianan , Tang Jianxiang , Du Xunbo , Peng Huanwen , Chen Xiao , Huang Tao , Liao Hongxiu , Xian Deqiang , Wang HaoZhou , Liu Wenwu , Zhou Ping , Zhang Zhengdong , Liu Juan , Wu Xianping , Zhang Tao
Published: October 2021

Objectives: To explore and understand the SARS-CoV-2 seroprevalence of convalescents, the association between antibody levels and demographic factors, and the seroepidemiology of convalescents of COVID-19 till March 2021.

Methods: We recruited 517 voluntary COVID-19 convalescents in Sichuan Province and collected 1,707 serum samples till March 2021. Then we reported the seroprevalence and analyzed the associated factors.

Results: Recent travel history was associated with IgM levels. Convalescents who had recent travel history were less likely to be IgM antibody negative [OR = 0.232, 95% CI: (0.128, 0.420)]. Asymptomatic cases had, approximately, twice the odds of being IgM antibody negative compared with symptomatic cases [OR = 2.583, 95% CI: (1.554, 4.293)]. Participants without symptoms were less likely to be IgG seronegative than those with symptoms [OR = 0.511, 95% CI: (0.293, 0.891)]. Convalescents aged 40–59 were less likely to be IgG seronegative than those aged below 20 [OR = 0.364, 95% CI: (0.138, 0.959)]. The duration of positive IgM antibodies persisted 365 days while the IgG persisted more than 399 days.

Conclusions: Our findings suggested that recent travel history might be associated with the antibody levels of IgM, while age could be associated with the antibody levels of IgG. Infection type could be associated with both antibody levels of IgM and IgG that declined quicker in asymptomatic cases.
Prevalence of the Acute Respiratory Infections and Associated Factors in the Rural Areas and Urban Slum Areas of Western Maharashtra, India: A Community-Based Cross-Sectional Study
Authors: Murarkar Sujata , Gothankar Jayashree , Doke Prakash , Dhumale Girish , Pore Prasad D. , Lalwani Sanjay , Quraishi Sanjay , Patil Reshma S. , Waghachavare Vivek , Dhobale Randhir , Rasote Kirti , Palkar Sonali , Malshe Nandini , Deshmukh Rupeshkumar
Published: October 2021

Acute respiratory infections (ARIs) continue to be the most important cause of morbidity and mortality among under-five children. Some demographic and environmental factors are associated with ARIs among under-five children. This study was conducted with the objective to estimate the prevalence of ARIs among under-five children in the rural areas and densely populated urban slum areas in Maharashtra, India and to assess the association of the selected sociodemographic and household environmental factors with ARI. This study was conducted in 16 selected clusters from the rural areas and densely populated urban slum areas of the two districts in Maharashtra, India. Structured and validated proforma was used for collecting the data on the sociodemographic and household environmental risk factors. A total of 3,671 under-five children were surveyed. The prevalence of ARIs for the preceding month was 50.4%. It was higher among the children living in the rural areas (54.2%) compared to the children living in the urban areas (46.7%) (p = 0.01). The prevalence of ARIs was reported to be 51.4 and 49.4% in boys and girls, respectively. In the multivariate analysis, the researchers found that living in rural areas (p = 0.01) and parental smoking (p = 0.04) were significantly associated with the ARIs. An intervention such as reducing parental smoking habits at the household level may reduce ARIs.
Combatting Substandard and Falsified Medicines: Public Awareness and Identification of Counterfeit Medications
Authors: El-Dahiyat Faris , Fahelelbom Khairi M. S. , Jairoun Ammar Abdulrahman , Al-Hemyari Sabaa Saleh
Published: October 2021

Objectives: The objective of this study was to determine the identification rate of substandard and falsified medications and its association with knowledge among public.

Methods: This descriptive cross-sectional study was conducted in different geographic areas among a convenient sample of people aged 18 or older. A validated web-based electronic questionnaire was used for data collection tool. The questionnaire contained three sections assessing the following: (1) Sociodemographic data; (2) Knowledge regarding counterfeit medicines; and (3) Ability to identify counterfeit medicines, according to 12 questions rated on a five-point Likert scale. Univariate and multivariate logistic regression analyses were used to assess the association between sociodemographic factors and counterfeit medication identification rate.

Results: A total of 320 people participated in the study. Only 98 participants (30.6%, 95% CI 25.6–35.7%) identified the counterfeit medications. Ability to correctly identify counterfeit medications was significantly higher in participants who were older (p = 0.016), single (p = 0.001), Asian (p = 0.001), or American (p = 0.019), as well as those who indicated that they would check the certification of the medications (p = 0.015) and report counterfeit medications to the authorities (p < 0.0001).

Conclusions: These results underscore the need for greater public awareness of the hazards associated with counterfeit medicines.
Ready, Set, Go! How the Heart and Lungs Respond to Exercise
Authors: Muise Eleanor D. , Gordon Rachel , Ericson Woods Dawn A.
Published: October 2021

You know when you’re racing really fast—so fast that your heart is pounding, and you can’t catch your breath? Have you ever wondered why that happens? Imagine you are racing—when you take a deep breath, you breathe in air and your lungs pull oxygen into your blood, then oxygen races to your hungry leg muscles. All that work by your muscles to make energy creates carbon dioxide waste, which travels back to the lungs, and we exhale with a sigh of relief. As you and your muscles work harder to run faster and further, your muscles require more oxygen to make more energy. Over time, with continued exercise, your heart and lungs respond by becoming more efficient at delivering oxygen and making energy. There are even ways to measure how hard your body is working during exercise, and this article will tell you how!
Adaptive Control of Chaotic Signals: Investigated by Simulation Software and Real Electronic Circuits
Authors: Yang Cheng-Hsiung , Chang Che-Lun , Li Shih-Yu
Published: October 2021

Chaotic behavior is complicated, sensitive, and has the feature of great variety, which are the most potential signals to be applied in data encryption, secure communication, medical information protection, etc. As a consequence, in this paper, we try to propose three different ways to show our data generating results step by step, which means it can be proved effectively and used in practice: (1) Chaotic solutions simulated by MATLAB, (2) chaotic motion drawn via electronic circuits software Multisim, and (3) chaotic signal implemented on real electronic circuits with breadboard. In advance, following the same design principal, the adaptive chaotic signal is also designed and presented in the end of this article for further study, which provides a more flexible and variable chaotic signal to enhance the encryption effectiveness. The experimental results are extremely close to the two simulation results and can definitely be technically transferred to real encryption application.
RETRACTED: A Closed-Loop Control Mathematical Model for Photovoltaic-Electrostatic Hybrid Actuator with a Slant Lower Electrode Based on PLZT Ceramic
Authors: Lv Zhen , Uzair Muhammad , Wang Xinjie , Liu Yafeng
Published: October 2021

In this paper, a novel photovoltaic-electrostatic hybrid actuator with a slant lower electrode based on the PLZT ceramic is proposed. The mathematical model of photovoltaic-electrostatic hybrid actuator is established. Then, based on the mathematical model of photovoltaic-electrostatic hybrid actuator and the parameters identified, the mathematical simulation of the closed-loop displacement control for the photovoltaic-electrostatic hybrid actuator based on the PLZT ceramic is carried out. The results show that the displacement of the actuator can be controlled successfully at a particular value within the pull-in displacement by the light source. Furthermore, the response speed of the output displacement for photovoltaic-electrostatic hybrid actuator with a slant lower electrode is faster than that with a parallel lower electrode, offering a good potential to advance the current applications on micro-electro-mechanical system.
Effect of Enterococcus faecium AL41 (CCM8558) and Its Enterocin M on the Physicochemical Properties and Mineral Content of Rabbit Meat
Authors: Pogány Simonová Monika , Chrastinová Ľubica , Lauková Andrea
Published: October 2021

Improving rabbit meat quality using natural substances has become an area of research activity in rabbit nutrition due to stabilization of husbandry health and economy. The present study evaluates the effect of bacteriocin-producing, beneficial strain Enterococcus faecium AL41/CCM8558 and its enterocin M (EntM) on the quality and mineral content of rabbit meat. Seventy-two Hycole rabbits (aged 35 days) were divided into EG1 (CCM8558 strain; 1.0 × 109 CFU/mL; 500 µL/animal/d), EG2 (EntM; 50 µL/animal/d), and control group (CG). The additives were administrated in drinking water for 21 days. Significant increase in meat phosphorus (EG1: p < 0.05; EG2: p < 0.0001) and iron (EG1, EG2: p < 0.001) contents was noted; sodium and zinc levels were only slightly higher in experimental groups compared with control data. The calcium (EG1, EG2: p < 0.001), potassium, and copper (EG1: p < 0.01) concentrations were reduced. The treatment did not have a negative influence on physicochemical traits of rabbit meat. Based on these results, we conclude that diet supplementation with beneficial strain E. faecium CCM8558 and its EntM could enhance the quality and mineral content of rabbit meat, with the focus on its iron and phosphorus contents.
Niche Shifts, Hybridization, Polyploidy and Geographic Parthenogenesis in Western North American Hawthorns (Crataegus subg. Sanguineae, Rosaceae)
Authors: Dickinson Timothy A. , Yan Brigitte Xueqi , Han Shery , Zarrei Mehdi
Published: October 2021

We compare biogeographic and morphological parameters of two agamic complexes of western North American hawthorns so as to evaluate possible explanations of the differences in range between sexually reproducing taxa and their apomictic sister taxa. We have documented range, breeding system, morphology, leaf vascular architecture, and niche breadth in these hawthorns, for which phylogenetic relationships and ploidy levels are known. Species distribution data from herbarium specimens and online databases were analyzed in order to compare ranges and climate niches described by bioclimatic variables. Flow cytometry documented ploidy level and breeding system. Voucher specimens provided morphometric data that were analyzed using uni- and multivariate methods. Members of two black-fruited taxonomic sections of Crataegus subg. Sanguineae (sections Douglasianae, Salignae) have previously been identified as hybrids. They are presumptively self-fertile polyploids with pseudogamous gametophytic apomixis. Their morphologies, geographic ranges, and niche characteristics resemble those of their diploid, sexual parent or are intermediate between them and those of their other parent, one or both of two partially sympatric tetraploid apomicts in red-fruited C. subg. Americanae with much wider distributions. Comparing sections Douglasianae and Salignae suggests that geographic parthenogenesis (larger range sizes in apomicts, compared to sexually reproducing taxa) may have less to do with adaptation than it does with reproductive assurance in the pseudogamously apomictic and self-compatible hybrids. Greater climate niche breadth in allopolyploids compared to diploids similarly may be more due to parental traits than to effects of genome duplication per se.
Yield Stability and Genotype Environment Interaction of Water Deficit Stress Tolerant Mung Bean (Vigna radiata L. Wilczak) Genotypes of Bangladesh
Authors: Islam Mohammad Rafiqul , Sarker Bikash Chandra , Alam Mohammad Ashraful , Javed Talha , Alam Mohammad Jahangir , Zaman Mohammad Shahin Uz , Azam Mohammad Golam , Shabbir Rubab , Raza Ali , Habib-ur-Rahman Muhammad , Dessoky Eldessoky S. , Islam Mohammad Sohidul
Published: October 2021

Water deficit stress is a critical abiotic constraint to mung bean production that affects plant growth and development and finally reduces crop yield. Therefore, a field experiment was conducted at five diverse environments using four water stress-tolerant genotypes, namely BARI Mung-8, BMX-08010-2, BMX-010015, and BMX-08009-7, along with two popular cultivated varieties (check) of BARI Mung-6 and BARI Mung-7 to evaluate more stable tolerant genotypes across the country. Stability analysis was performed based on the grain yield. The combined analysis of variance showed significant variations among genotypes, environments, and their interactions. The AMMI analysis of variance indicated that genotype accounted for 91% of the total sum of squares for grain yield, followed by genotype × environment interaction (5%), and environment (4%). Partitioning of interaction indicated that the first three interaction principal components (IPCA1–IPCA3) were highly significant (p ≤ 0.01). Using these significant IPCAs, AMMI stability parameters and non-parameter indices BMX-010015 was found stable across the environment based on yield traits and grain yield. The BMX-08010-2 genotype also showed significant regression coefficient (bi) more than unity, and non-significant deviation from regression (S2di) values, indicating suitable for a favorable environment considering grain yield. So, based on the stability analysis (Eberhart and Russell), additive main effects, and multiplicative interactions (AMMI) analysis, the BMX-010015 and BMX-08010-2 could be suitable for having tolerance to water deficit stress.
Antibiotics in Orthopedic Infections
Authors: Anagnostakos Konstantinos , Fink Bernd
Published: October 2021

The management of orthopedic infections has continuously been gaining increasing interest in the past few years [...]
Local/Topical Antibiotics for Peri-Implantitis Treatment: A Systematic Review
Authors: Passarelli Pier Carmine , Netti Andrea , Lopez Michele Antonio , Giaquinto Eleonora Favetti , De Rosa Giuseppe , Aureli Gianmarco , Bodnarenko Alina , Papi Piero , Starzyńska Anna , Pompa Giorgio , D’Addona Antonio
Published: October 2021

Most studies indicate that the mechanical removal of the bacterial biofilm from the implant surface is the central goal of peri-implantitis therapy. However, controversial results in the treatment of peri-implantitis have led to the consideration of additional strategies that include surgical approaches and chemical adjuvants. Local/topical antibiotics, such as minocycline, azithromycin, tetracycline, amoxicillin, doxycycline, and metronidazole, may improve the efficacy of the definitive treatment of the disease, but the lack of conclusive findings prevents their use in clinical practice. This systematic review aimed to evaluate the effect of local/topical antibiotics for peri-implantitis treatment. Randomised controlled studies (RCT) on patients with peri-implantitis and comparing the efficacy of local/topical antibiotics vs. placebo or mechanical debridement were included. A systematic search strategy was carried out using three registered databases (PubMed, Web of Science, and Scopus). RoB2 was used to assess risk of bias. Five RCTs were identified (n = 250 patients and 333 implants). Contrast results emerged among the included studies, and a high heterogeneity level was observed. Risk of bias revealed some concerns for three studies out of five, while one study was judged at high risk. Only one study analysed the limitations of its findings. Overall, local antibiotic use can be considered a valid approach in the treatment of peri-implantitis. Therefore, future long-term clinical trials with standardised protocols and antibiotics with similar biological activity profiles should be tested to achieve a valid and definitive conclusion.
Thunbergia laurifolia Leaf Extract Inhibits Glutamate-Induced Neurotoxicity and Cell Death through Mitophagy Signaling
Authors: Vongthip Wudtipong , Sillapachaiyaporn Chanin , Kim Kyu-Won , Sukprasansap Monruedee , Tencomnao Tewin
Published: October 2021

Oxidative stress plays a crucial role in neurodegeneration. Therefore, reducing oxidative stress in the brain is an important strategy to prevent neurodegenerative disorders. Thunbergia laurifolia (Rang-jued) is well known as an herbal tea in Thailand. Here, we aimed to determine the protective effects of T. laurifolia leaf extract (TLE) on glutamate-induced oxidative stress toxicity and mitophagy-mediated cell death in mouse hippocampal cells (HT-22). Our results reveal that TLE possesses a high level of bioactive antioxidants by LC–MS technique. We found that the pre-treatment of cells with TLE prevented glutamate-induced neuronal death in a concentration-dependent manner. TLE reduced the intracellular ROS and maintained the mitochondrial membrane potential caused by glutamate. Moreover, TLE upregulated the gene expression of antioxidant enzymes (SOD1, SOD2, CAT, and GPx). Interestingly, glutamate also induced the activation of the mitophagy process. However, TLE could reverse this activity by inhibiting autophagic protein (LC3B-II/LC3B-I) activation and increasing a specific mitochondrial protein (TOM20). Our results suggest that excessive glutamate can cause neuronal death through mitophagy-mediated cell death signaling in HT-22 cells. Our findings indicate that TLE protects cells from neuronal death by stimulating the endogenous antioxidant enzymes and inhibiting glutamate-induced oxidative toxicity via the mitophagy–autophagy pathway. TLE might have potential as an alternative or therapeutic approach in neurodegenerative diseases.
Over-Expression of Chorismate Mutase Enhances the Accumulation of Salicylic Acid, Lignin, and Antioxidants in Response to the White-Backed Planthopper in Rice Plants
Authors: Jan Rahmatullah , Khan Muhammad Aaqil , Asaf Sajjad , Lubna  , Lee In-Jung , Kim Kyung-Min
Published: October 2021

The white-backed planthopper (WBPH) is a serious pest of rice crop and causes sever yield loss each year, especially in Asian countries. In this study, we used chorismate mutase (CM) transgenic line to examine the defense mechanism of rice plants against WBPH. The survival rate of WBPHs, infestation rate of plants, lignin biosynthesis, transcriptional regulation of related genes, salicylic acid (SA) accumulation and signaling and antioxidants regulation were investigated. The WBPH population decreased by 67% in OxCM-t, and the plant infestation rate was 3.5-fold higher in wild-type plants compared with transgenic plants. A substantial increase in lignin was found in the transgenic line (742%) and wild-type (417%) plants. Additionally, CM, phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), and chalcone isomerase (CHI) showed significant increases in their relative expression level in the transgenic line. Salicylic acid was significantly enhanced in the transgenic line compared with WBPH infestation. SA can activate pathogenesis related proteins-1 (PR1), PR2, antioxidants, and the expression of their related genes: superoxide dismutase (SOD) and catalase (CAT). WBPH infestation reduced the chlorophyll contents of both transgenic and wild-type plants, but the reduction was great in wild-type than transgenic plants. The sugar content was only significantly increased in the transgenic line, indicating that sugars are not heavily involved in WBPH stress. Phenylalanine, proline, aspartic acid, and total amino acids were increased in the transgenic line and reduced in the wild-type plants. Taken together, all the results suggest that overexpression of CM gene regulates the defense mechanisms and enhances the rice toward WBPH stress.
CO2 Supercritical Fluid Extraction of Oleoresins from Sea Buckthorn Pomace: Evidence of Advanced Bioactive Profile and Selected Functionality
Authors: Mihalcea Liliana , Turturică Mihaela , Cucolea Elena Iulia , Dănilă George-Mădălin , Dumitrașcu Loredana , Coman Gigi , Constantin Oana Emilia , Grigore-Gurgu Leontina , Stănciuc Nicoleta
Published: October 2021

The processing of sea buckthorn generates a significant amount of pomace, seeds and skin considered valuable sources of health-promoting macromolecules, such as carotenoids, pectin, flavonoids, phytosterols, polyunsaturated fatty acids and tocopherols. In this study, the bioactives from sea buckthorn pomace (SBP) were extracted using supercritical carbon dioxide (SFE-CO2), at different temperatures and pressures, allowing for obtaining four fractions according to separators (S40 and S45). The highest carotenoid content of 396.12 ± 1.02 mg/g D.W. was found in the S40 fraction, at extraction parameters of 35 °C/45 MPa, yielding an antioxidant activity of 32.10 ± 0.17 mMol TEAC/g D.W. The representative carotenoids in the extract were zeaxanthin, β-carotene and lycopene, whereas all enriched SFE-CO2 extracts contained α-, β- and δ-tocopherol, with α-tocopherol representing around 82% of all fractions. β-sitosterol was the major phytosterol in the fractions derived from S45. All fractions contained significant fatty acids, with a predominance of linoleic acid. Remarkably, the enriched extracts showed a significant palmitoleic acid content, ranging from 53 to 65 µg/g. S40 extracts showed a good antibacterial activity against Staphylococcus aureus and Aeromonas hydrophila ATCC 7966, whereas S45 extracts showed a growth inhibition rate of 100% against Aspergillus niger after three days of growth. Our results are valuable, and they allow identifying the different profiles of extracts with many different applications in food, pharmaceutics, nutraceuticals and cosmeceuticals.
17β-Estradiol Abrogates Oxidative Stress and Neuroinflammation after Cortical Stab Wound Injury
Authors: Saeed Kamran , Jo Myeung Hoon , Park Jun Sung , Alam Sayed Ibrar , Khan Ibrahim , Ahmad Riaz , Khan Amjad , Ullah Rahat , Kim Myeong Ok
Published: October 2021

Disruptions in brain energy metabolism, oxidative damage, and neuroinflammation are commonly seen in traumatic brain injury (TBI). Microglial activation is the hallmark of neuroinflammation. After brain injury, microglia also act as a double-edged sword with distinctive phenotypic changes. Therefore, therapeutic applications to potentiate microglia towards pro-inflammatory response following brain injury have become the focus of attention in recent years. Here, in the current study, we investigated the hypothesis that 17β-estradiol could rescue the mouse brain against apoptotic cell death and neurodegeneration by suppressing deleterious proinflammatory response probably by abrogating metabolic stress and oxidative damage after brain injury. Male C57BL/6N mice were used to establish a cortical stab wound injury (SWI) model. Immediately after brain injury, the mice were treated with 17β-estradiol (10 mg/kg, once every day via i.p. injection) for one week. Immunoblotting and immunohistochemical analysis was performed to examine the cortical and hippocampal brain regions. For the evaluation of reactive oxygen species (ROS), reduced glutathione (GSH), and oxidized glutathione (GSSG), we used specific kits. Our findings revealed that 17β-estradiol treatment significantly alleviated SWI-induced energy dyshomeostasis and oxidative stress by increasing the activity of phospho-AMPK (Thr172) and by regulating the expression of an antioxidant gene (Nrf2) and cytoprotective enzymes (HO-1 and GSH) to mitigate ROS. Importantly, 17β-estradiol treatment downregulated gliosis and proinflammatory markers (iNOS and CD64) while significantly augmenting an anti-inflammatory response as evidenced by the robust expression of TGF-β and IGF-1 after brain injury. The treatment with 17β-estradiol also reduced inflammatory mediators (Tnf-α, IL-1β, and COX-2) in the injured mouse. Moreover, 17β-estradiol administration rescued p53-associated apoptotic cell death in the SWI model by regulating the expression of Bcl-2 family proteins (Bax and Bcl-2) and caspase-3 activation. Finally, SWI + 17β-estradiol-treated mice illustrated reduced brain lesion volume and enhanced neurotrophic effect and the expression of synaptic proteins. These findings suggest that 17β-estradiol is an effective therapy against the brain secondary injury-induced pathological cascade following trauma, although further studies may be conducted to explore the exact mechanisms.
Exploiting the Anti-Inflammatory Potential of White Capsicum Extract by the Nanoformulation in Phospholipid Vesicles
Authors: Pappalardo Ilaria , Santarsiero Anna , De Luca Maria , Acquavia Maria Assunta , Todisco Simona , Caddeo Carla , Bianco Giuliana , Infantino Vittoria , Martelli Giuseppe , Vassallo Antonio
Published: October 2021

The peppers of the Capsicum species are exploited in many fields, as flavoring agents in food industry, or as decorative and therapeutic plants. Peppers show a diversified phytochemical content responsible for different biological activities. Synergic activity exerted by high levels of antioxidant compounds is responsible for their important anti-inflammatory property. A methanolic extract was obtained from a new pepper genotype and tested for anti-inflammatory activity. The extract was incorporated into phospholipid vesicles to increase the bioavailability of its bioactive components. Two types of phospholipid vesicles were produced, conventional liposomes and Penetration Enhancer containing Vesicles (PEVs). They were tested in human monoblastic leukemia U937 cell line, showing no cytotoxic effect. The intracellular reactive oxygen species (ROS) and nitric oxide (NO) levels were measured to value the in vitro efficacy of the vesicles in regulating inflammatory responses. Liposomal incorporation significantly reduced ROS levels in extract-treated LPS-activated cells. Furthermore, LC-MS/MS analyses demonstrated that liposomes facilitated the transport of the extract components across the cell membrane and their accumulation into the cytoplasm.
Does the Intraluminal Thrombus Provoke the Rupture of the Abdominal Aortic Aneurysm Wall?
Authors: Almijalli Mohammed
Published: October 2021

The role of intraluminal thrombus (ILT) in the rupture of abdominal aortic aneurysms (AAA) is controversial, and it is unclear whether it increases or decreases the risk of rupture. This research aims to find a clear answer to this question. Previous computer modelling suggests that an ILT lowers oxygen dissemination to the AAA wall, contributing to wall thinning. The methodology used in this study determines the amount of oxygen reaching the aneurysm wall after passing through the ILT by using the porous nature of the ILT to recreate the condition as closely as feasible. Using computed tomographic images, patient-specific three-dimensional (3D) AAA geometries were recreated. Modelling blood and oxygen flow in AAA was obtained using a computational fluid dynamics (CFD) approach. Our findings indicated that the oxygen volume percentage had completely reached the aneurysm wall. Only at the inlet and outflow did the greatest wall shear stress (WSS) occur, with a significant drop in the central region of the aneurysm wall. CFD was used to calculate the velocity, pressure, and WSS of aortic blood flow. ILT had no effect on oxygen flow to the aneurysm wall, disproving the theory that it produces local hypoxia.
Effect of Boundary Conditions on the Mechanical Behavior of the Geogrid–Soil Interface
Authors: Zuo Zheng , Yang Guangqing , Wang Zhijie , Wang He , Jin Jing
Published: October 2021

Geogrid-reinforced structures are extensively adopted in various engineering fields. At present, the influence of boundary conditions was not considered in design methods, bringing hidden dangers to the safety of the structure. In the current study, a series of pullout tests were carried out on high-density polyethylene (HDPE) geogrid-reinforced coarse sand. The magnitude and growth pattern of pullout resistance and the variation laws of interfacial shear strength indexes under four types of boundary conditions were analyzed. Additionally, the boundary reduction coefficient (BRC) was introduced to establish the relationship between rigid and flexible boundary for the design of the structure. The tests results showed that the boundary conditions cannot be ignored in the design of structures, especially in the front. When the normal loading was up to 120 kPa, the BRC-top and BRC-positive could be taken as 0.9 and 0.5, respectively, and verified by fitting results. The boundary conditions affected the pullout resistance, while the vertical loading corresponding to the maximum pullout resistance was not related to boundary conditions. Investigating the interaction of the geogrid–soil under different boundary conditions can help to improve the understanding of the behavior of reinforced soil structure, and to achieve a more efficient and economical design.
Antiproliferative Activity of Stokesia laevis Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies
Authors: Neagu Georgeta , Stefaniu Amalia , Albulescu Adrian , Pintilie Lucia , Pirvu Lucia Camelia
Published: October 2021

This study evaluates in vitro cytotoxic and antiproliferative activity on human colon tumor cell line Caco-2 (ATCC-HTB-37) of a standardized (5 mg GAE/mL) ethanolic extract from Stokesia laevis (Slae26), of five polyphenols compounds (reference substances, ref.), namely luteolin-7-O-glucoside, luteolin-8-C-glucoside, caffeic acid, gentisic acid, and p-aminobenzoic acid (PABA), as well as of Slae26 combinations with the five reference substances, 1:1 mass rate (GAE, ref.). Cell viability studies (MTS test) have revealed IC50 values of 36 μg GAE/mL in the case of Slae26 ethanolic extract, while Slae26 combinations with the five phenolics indicated IC50 values around 5 μg GAE/mL. In silico docking studies on the molecular targets human tankyrase 1 (TNKS1) and human tankyrase 2 (TNKS2) in complex with their native ligands, Co-crystallized 3J5A and Co-crystallized FLN, indicated score values of −104.15 and −76.97, respectively; in the series of the reference compounds studied, luteolin-7-O-glucoside was revealed with the best score values on both molecular targets (−80.49 and −85.17), together signifying real antiproliferative potential against human colon cancer of Slae26, of luteolin-7-O-glucoside, and of Slae26 combinations with all food-related bioactive compounds tested.
A Novel Convolutional Neural Network Classification Approach of Motor-Imagery EEG Recording Based on Deep Learning
Authors: Echtioui Amira , Mlaouah Ayoub , Zouch Wassim , Ghorbel Mohamed , Mhiri Chokri , Hamam Habib
Published: October 2021

Recently, Electroencephalography (EEG) motor imagery (MI) signals have received increasing attention because it became possible to use these signals to encode a person’s intention to perform an action. Researchers have used MI signals to help people with partial or total paralysis, control devices such as exoskeletons, wheelchairs, prostheses, and even independent driving. Therefore, classifying the motor imagery tasks of these signals is important for a Brain-Computer Interface (BCI) system. Classifying the MI tasks from EEG signals is difficult to offer a good decoder due to the dynamic nature of the signal, its low signal-to-noise ratio, complexity, and dependence on the sensor positions. In this paper, we investigate five multilayer methods for classifying MI tasks: proposed methods based on Artificial Neural Network, Convolutional Neural Network 1 (CNN1), CNN2, CNN1 with CNN2 merged, and the modified CNN1 with CNN2 merged. These proposed methods use different spatial and temporal characteristics extracted from raw EEG data. We demonstrate that our proposed CNN1-based method outperforms state-of-the-art machine/deep learning techniques for EEG classification by an accuracy value of 68.77% and use spatial and frequency characteristics on the BCI Competition IV-2a dataset, which includes nine subjects performing four MI tasks (left/right hand, feet, and tongue). The experimental results demonstrate the feasibility of this proposed method for the classification of MI-EEG signals and can be applied successfully to BCI systems where the amount of data is large due to daily recording.
Analysis of Layered Geogrids–Sand–Clay Reinforced Structures under Triaxial Compression by Discrete Element Method
Authors: Cui Lan , Cao Wenzhao , Sheng Qian , Xie Mingxing , Yang Tao , Xiao Ping
Published: October 2021

Compared with the commonest geosynthetics-reinforced soil structures, layered geogrids–sand–clay reinforced (LGSCR) structures (School of Architecture and Civil Engineering, Shenyang University of Technology, Shenyang 110870, China) can replace granular materials with clay as the primary backfill material. Up until now, the performance of LGSCR structures under triaxial compression has been unclear. In this paper, the discrete element method was used to simulate the triaxial compression test on the LGSCR samples. Based on the particle flow software PFC3D, three types of cluster particle-simulated sand and the reinforced joints of the geogrid were constructed by secondary development. The effects of the geogrid embedment in sand layers, the number and thickness of sand layers in relation to the deviatoric stress, and the axial strain and the shear strength index of the LGSCR samples were analyzed. The results showed that laying the sand layers in the samples can improve their post-peak strain-softening characteristics and increase their peak strengths under a high confining pressure. A geogrid embedment in sand layers can further enhance the ductility and peak strength of the samples, and in terms of the shear strength index, there is a 41.6% to 54.8% increase in the apparent cohesion of the samples.
Design of a Printed 5G Monopole Antenna on Vehicle Window Glass Using Parasitic Elements and a Lattice-Structure Reflector for Gain Enhancement
Authors: Im Changhyeon , Lim Tae-Heung , Jang Doyoung , Kong Nak-Kyoung , Choo Hosung
Published: October 2021

This letter proposes a novel design for a printed 5G monopole antenna on a vehicle window glass. The proposed antenna consists of a coplanar waveguide (CPW), a monopole radiator, parasitic elements, and a lattice-structure reflector. The parasitic elements are placed on either side of the monopole radiator to improve the bore-sight gain. To solve the radiation pattern distortion problem that occurs due to the thick vehicle window glass, the lattice-structure reflector is printed on the opposite side of the monopole radiator. Through fabrication and measurement of the proposed antenna, it is confirmed that the design improves bore-sight gain, and minimizes the radiation pattern distortion. The results demonstrate that the proposed 5G monopole antenna with parasitic elements and the lattice-structure reflector is suitable for 5G communication in vehicle applications.
Experimental Study and Simulation of the Stress Relaxation Characteristics of Machine-Harvested Seed Cotton
Authors: Wang Jun , Zhang Hongwen , Wang Lei , Wei Ximei , Wang Meng , Gu Yanqing , Cai Yunxiao
Published: October 2021

Seed cotton compression molding solves the inconvenience of seed cotton transportation and storage after mechanical harvesting. Stress relaxation is closely related to the performance of the compressed seed cotton. In this study, an electronic universal testing machine with a homemade compression device was used to study the stress relaxation characteristics of machine-harvested seed cotton. The stress relaxation model of machine-harvested seed cotton was established, the influence of test factors on the response indexes was analyzed and, finally, stress relaxation characteristics of machine-harvested seed cotton were simulated. Results show that machine-harvested seed cotton stress relaxation characteristics can be described by the five-element Maxwell model. The equilibrium elastic modulus is negatively correlated with moisture content and cross-section dimensions, and the equilibrium elastic modulus is positively correlated with trash content and compression density. The rapid decay time and the residual stress ratio are negatively correlated with moisture content and compression density, but the influence of trash content and cross-section dimensions are limited. The stress relaxation process of machine-harvested seed cotton was simulated using virtual prototype technology, and the maximum error between the experimental and simulated values was obtained as 4.96%. The feasibility of the virtual prototype technique for the viscoelastic simulation of biomaterials was demonstrated.
The Assessment of the Maximum Heat Production and Cooling Effectiveness of Three Different Drill Types (Conical vs. Cylindrical vs. Horizontal) during Implant Bed Preparation—An In Vitro Study
Authors: Ihde Stefan , Dalewski Bartosz , Pałka Łukasz
Published: October 2021

The aim of this experimental study was to verify thermal diffusion differences, by measuring the maximum temperature achieved with different drill shapes. Synthetic bone blocks of type I density made from solid rigid polyurethane (PUR) foam were used to perform the drilling procedures. The experiment was conducted at three different rotation speeds: 800, 3000 and 5000 rpm. Conical drills (with and without an internal cooling hole) were compared with horizontal drills and disc drills. The temperature during drilling for implant bed preparation was estimated with the use of thermocouples and an infrared (IR) camera. The temperature during drilling with disc cutters for lateral basal implants did not exceed 33 ∘C and the temperature decreased in proportion to higher drill speed. The results indicate that the tested design is safe and will not cause bone overheating.
Implementing a Circadian Adaptation Schedule after Eastward Flight in Young Male Athletes
Authors: Miller Dean J. , Roach Gregory D. , Lastella Michele , Scanlan Aaron T. , Sargent Charli
Published: October 2021

This study examined the effectiveness of a circadian adaptation schedule in male cricketers after an 8.5 h eastward time zone change. Ten participants (aged 18.7 ± 0.9 y) were randomly assigned to a control group or an intervention group. Participants in the intervention group followed a light exposure schedule in which they were instructed to seek light in the three hours preceding, and avoid light in the three hours following their estimated core body temperature minimum. The rate of adaptation was assessed using the nightly excretion rate of urinary 6-sulphatoxymelatonin (aMT6s). General linear mixed models were conducted to assess the effect of condition (i.e., control and light intervention) on nocturnal secretion of aMT6s. Significant main effects of day (F(7, 35) = 10.4, p < 0.001) were reflected by an increase in nocturnal melatonin excretion (i.e., all participants gradually adapted to the destination time zone). Subjective jet lag decreased by day (F(7, 54) = 22.9, p < 0.001), bedtime was delayed by day (F(7, 54) = 3.1, p = 0.007) and get up time was earlier by day (F(7, 35) = 5.4, p < 0.001). On average, it took 7 days for all participants to return to baseline levels following transmeridian travel. Similarly, it took 7 days for subjective jet lag to alleviate. In the initial 4 days of the protocol, the intervention group registered higher levels of nocturnal urinary melatonin, however, there was no significant differences in the rate of adaptation between the groups. It is possible that participants did not adhere to the intervention or that they followed the intervention but it was ineffective.
Comparison of the Chemical and Sensorial Evaluation of Dark Chocolate Bars
Authors: Pieracci Ylenia , Ascrizzi Roberta , Pistelli Luisa , Flamini Guido
Published: October 2021

As it mimics olfactory perception, headspace analysis is frequently used for examination of products like chocolate, in which aroma is a key feature. Chemical analysis by itself, however, only provides half the picture, as final consumer’s perception cannot be compared to that of a Gas Chromatography-Mass Spectrometry (GC-MS) port, but rather to a panel test assessment. The aim of the present study was the evaluation of combined chemical (by means of headspace solid-phase microextraction and GC-MS) and panel test data (by means of a sensory evaluation operated by 6 untrained panelists) obtained for 24 dark chocolate bars to assess whether these can discriminate between bars from different brands belonging to different commercial segments (hard discount, HD; supermarket, SM; organic bars, BIO). In all samples, with the only exception of one supermarket bar (in which esters exhibited the highest relative abundance), pyrazines were detected as the most abundant chemical class (HD: 56.3–74.2%; BIO: 52.0–76.4%; SM: 31.2–88.9%). Non-terpene alcohols, aldehydes, and esters followed as quantitatively relevant groups of compounds. The obtained data was then subjected to hierarchical cluster (HCA) and principal component (PCA) analysis. The statistical distribution of samples obtained for the chemical data did not match that obtained with panelists’ sensorial data. Moreover, although an overall ability of grouping samples of the same commercial origin was evidenced for hard discount and supermarket bars, no sharp grouping was possible.