bluuebunny/crossref_metadata_2025 huggingface dataset:
Quality-based guidance for exploratory dimensionality reduction
Authors: Fernstad Sara Johansson , Shaw Jane , Johansson Jimmy
Published: October 2012

High-dimensional data sets containing hundreds of variables are difficult to explore, as traditional visualization methods often are unable to represent such data effectively. This is commonly addressed by employing dimensionality reduction prior to visualization. Numerous dimensionality reduction methods are available. However, few reduction approaches take the importance of several structures into account and few provide an overview of structures existing in the full high-dimensional data set. For exploratory analysis, as well as for many other tasks, several structures may be of interest. Exploration of the full high-dimensional data set without reduction may also be desirable. This paper presents flexible methods for exploratory analysis and interactive dimensionality reduction. Automated methods are employed to analyse the variables, using a range of quality metrics, providing one or more measures of ‘interestingness’ for individual variables. Through ranking, a single value of interestingness is obtained, based on several quality metrics, that is usable as a threshold for the most interesting variables. An interactive environment is presented in which the user is provided with many possibilities to explore and gain understanding of the high-dimensional data set. Guided by this, the analyst can explore the high-dimensional data set and interactively select a subset of the potentially most interesting variables, employing various methods for dimensionality reduction. The system is demonstrated through a use-case analysing data from a DNA sequence-based study of bacterial populations.
Mediational tools in story construction: An investigation of cultural influences on children’s narratives
Authors: Khimji Fatima , Maunder Rachel E
Published: October 2012

In this article we investigate how the content of children’s stories can provide insight into their cultural contexts. Informed by sociocultural theory, we use children’s narrative as a methodological tool for understanding the role of cultural influences in their construction of personal experiences and imaginary events. Twelve children in a year one class (five–six years) participated in pairs in three storytelling activities designed to draw on both imaginary scenarios and real world experiences. First, a picture was used as a stimulus and children were asked to formulate a story about what was happening in the image. Second, children were read the first part of a story book, and then asked to explain how they thought the story would finish. Finally, children were asked to recount an experience about a holiday they had been on. Thematic analysis of the stories illustrated how children utilized meditational tools to construct their narratives. Children’s imaginary capabilities were closely entwined with their own personal experiences and developmental context and they drew on beliefs and practices that were culturally situated. As a result we suggest that incorporating storytelling activities into early years classrooms can enable educators to develop a closer understanding of pupils’ cultural development, and provide researchers with a valuable methodological resource for studying sociocultural perspectives.
Difficult decisions
Authors: Huneke Nathan T.M. , Gupta Rupa , Halder Neel , Chaudhry Nasim
Published: October 2012

Patients with learning disabilities are not always involved in decision-making about their medications. This may mean that some patients are unfairly denied of their autonomy. We carried out an audit of current practice concerning consent to treatment in patients with learning disabilities against best practice guidelines. Data were collected via a questionnaire given to a sample of 70 patients with learning disabilities within the Salford catchment area. This questionnaire assessed whether patients were involved in decision-making regarding their medications and whether they were being given enough information to give informed consent. A total of 45 patients completed questionnaires. Overall, the patients’ knowledge of their medications was poor, particularly of the proposed duration, possible disadvantages and name of the treatment. It appears that doctors are engaging these patients during consultations and discussing their medications. However, the delivery of this information needs to be improved, and patients’ understanding and recall need to be checked more thoroughly.
Rituximab combination therapy in relapsing multiple sclerosis
Authors: Cross Anne H. , Klein Robyn S. , Piccio Laura
Published: October 2012

In multiple sclerosis (MS), the presence of B cells, plasma cells and excess immunoglobulins in central nervous system lesions and in the cerebrospinal fluid implicate the humoral immune system in disease pathogenesis. However, until the advent of specific B-cell-depleting therapies, the critical role of B cells and their products in MS was unproven. Rituximab, a monoclonal antibody that depletes B cells by targeting the CD20 molecule, has been shown to effectively reduce disease activity in patients with relapsing MS as a single agent. Our investigator-initiated phase II study is the only published clinical trial in which rituximab was used as an add-on therapy in patients with relapsing MS who had an inadequate response to standard injectable disease-modifying therapies (DMTs). The primary endpoint, magnetic resonance imaging (MRI) gadolinium-enhanced (GdE) lesion number before versus after rituximab, showed significant benefit of rituximab (74% of post-treatment MRI scans being free of GdE lesions compared with 26% free of GdE lesions at baseline; p < 0.0001). No differences were noted comparing patients on different DMTs. Several secondary clinical endpoints, safety and laboratory measurements (including B- and T-cell numbers in the blood and cerebrospinal fluid (CSF), serum and CSF chemokine levels, antibodies to myelin proteins) were assessed. Surprisingly, the decline in B-cell number was accompanied by a significant reduction in the number of T cells in both the peripheral blood and CSF. Rituximab therapy was associated with a significant decline of two lymphoid chemokines, CXCL13 and CCL19. No significant changes were observed in serum antibody levels against myelin proteins [myelin basic protein (MBP) and myelin/oligodendrocyte glycoprotein (MOG)] after treatment. These results suggest that B cells play a role in MS independent from antibody production and possibly related to their role in antigen presentation to T cells or to their chemokine/cytokine production.
Resolution of Meta-Accuracy
Authors: Carlson Erika N. , Furr R. Michael
Published: October 2012

Do people know when they can trust their metaperceptions (i.e., their beliefs about how they are seen)? The current study is the first to examine whether people can recognize which of their metaperceptions are more or less accurate, and it examines the source of this “resolution.” In two samples, we assessed meta-accuracy, or the degree to which people’s beliefs about the impressions they made corresponded to the actual impressions they made, for several close acquaintances (e.g., family, friends). We also assessed people’s confidence in the accuracy of their metaperceptions for each acquaintance. Results showed that people recognized when they were more or less “meta-accurate,” particularly in terms of the ways in which they were perceived as distinctive and unique individuals. This ability was partially driven by relationship quality. In sum, people seem to know when to trust their metaperceptions about individuals from their everyday lives.
Drug treatment developments in schizophrenia and bipolar mania: latest evidence and clinical usefulness
Authors: Johnsen Erik , Kroken Rune A.
Published: October 2012

Schizophrenia and bipolar disorder are often highly debilitating with chronic courses, and psychotropic drugs represent cornerstones in the treatment. The primary aim of the review was to summarize the latest evidence with regards to the efficacy and effectiveness of drug treatment of schizophrenia and the manic phases of bipolar disorder. Schizophrenia systematic reviews conclude that antipsychotic drugs are effective in treating overall symptoms of psychosis and in preventing relapse. Some of the newer agents, the second-generation antipsychotics (SGAs), have demonstrated superiority compared with the older first-generation drugs and other SGAs but side-effect differences among the drugs are of a greater magnitude than effect differences. The pragmatic randomized trials of effectiveness have shown a longer time until treatment discontinuation for olanzapine compared with other antipsychotics. Cohort studies have found superiority for the long-acting injection formulations compared with the oral formulations of the drugs, and lower total mortality risk in users of antipsychotics compared with non-users. In bipolar mania SGAs have shown superior antimanic efficacy compared with other mood-stabilizing drugs. In conclusion antipsychotics, in particular some of the SGAs, seem to be drugs of first choice for both schizophrenia and bipolar mania. This perspective review focused on mean effects but the group means may not always be particularly useful as schizophrenia and bipolar mania are biologically heterogeneous disorders with large inter-individual variations in drug response and tolerance. In patients with a prior drug history the different pharmacological and clinical profiles may be exploited in subsequent choices of drugs.
Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury
Authors: Gil Sucheol , Farnand Alex W , Altemeier William A , Gill Sean E , Kurdowska Anna , Krupa Agnieszka , Florence Jon M , Matute-Bello Gustavo
Published: October 2012

Background

Exposure to mechanical ventilation enhances lung injury in response to various stimuli, such as bacterial endotoxin (LPS). The Fas/FasL system is a receptor ligand system that has dual pro-apoptotic and pro-inflammatory functions and has been implicated in the pathogenesis of lung injury. In this study we test the hypothesis that a functioning Fas/FasL system is required for the development of lung injury in mechanically ventilated mice.

Methods

C57BL/6 (B6) and Fas-deficientlprmice were exposed to either intra-tracheal PBS followed by spontaneous breathing or intra-tracheal LPS followed by four hours mechanical ventilation with tidal volumes of 10 mL/kg, respiratory rate of 150 breaths per minute, inspired oxygen 0.21 and positive end expiratory pressure (PEEP) of 3 cm of water.

Results

Compared with the B6 mice, thelprmice showed attenuation of the neutrophilic response as measured by decreased numbers of BAL neutrophils and lung myeloperoxidase activity. Interestingly, the B6 andlprmice had similar concentrations of pro-inflammatory cytokines, including CXCL1 (KC), and similar measurements of permeability and apoptosis. However, the B6 mice showed greater deposition of anti-KC:KC immune complexes in the lungs, as compared with thelprmice.

Conclusions

We conclude that a functioning Fas/FasL system is required for full neutrophilic response to LPS in mechanically ventilated mice.
Frnakenstein: multiple target inverse RNA folding
Authors: Lyngsø Rune B , Anderson James WJ , Sizikova Elena , Badugu Amarendra , Hyland Tomas , Hein Jotun
Published: October 2012

Background

RNA secondary structure prediction, or folding, is a classic problem in bioinformatics: given a sequence of nucleotides, the aim is to predict the base pairs formed in its three dimensional conformation. The inverse problem of designing a sequence folding into a particular target structure has only more recently received notable interest. With a growing appreciation and understanding of the functional and structural properties of RNA motifs, and a growing interest in utilising biomolecules in nano-scale designs, the interest in the inverse RNA folding problem is bound to increase. However, whereas the RNA folding problem from an algorithmic viewpoint has an elegant and efficient solution, the inverse RNA folding problem appears to be hard.

Results

In this paper we present a genetic algorithm approach to solve the inverse folding problem. The main aims of the development was to address the hitherto mostly ignored extension of solving the inverse folding problem, the multi-target inverse folding problem, while simultaneously designing a method with superior performance when measured on the quality of designed sequences. The genetic algorithm has been implemented as a Python program called Frnakenstein. It was benchmarked against four existing methods and several data sets totalling 769 real and predicted single structure targets, and on 292 two structure targets. It performed as well as or better at finding sequences which folded in silico into the target structure than all existing methods, without the heavy bias towards CG base pairs that was observed for all other top performing methods. On the two structure targets it also performed well, generating a perfect design for about 80% of the targets.

Conclusions

Our method illustrates that successful designs for the inverse RNA folding problem does not necessarily have to rely on heavy biases in base pair and unpaired base distributions. The design problem seems to become more difficult on larger structures when the target structures are real structures, while no deterioration was observed for predicted structures. Design for two structure targets is considerably more difficult, but far from impossible, demonstrating the feasibility of automated design of artificial riboswitches. The Python implementation is available athttp://www.stats.ox.ac.uk/research/genome/software/frnakenstein.
Molecular characteristics of erythromycin-resistant Streptococcus pneumoniaefrom pediatric patients younger than five years in Beijing, 2010
Authors: Zhou Lin , Ma Xiang , Gao Wei , Yao Kai-hu , Shen A-dong , Yu Sang-jie , Yang Yong-hong
Published: October 2012

Background

Streptococcus pneumoniae is the main pathogen that causes respiratory infections in children younger than five years. The increasing incidence of macrolide- and tetracycline-resistant pneumococci among children has been a serious problem in China for many years. The molecular characteristics of erythromycin-resistant pneumococcal isolates that were collected from pediatric patients younger than five years in Beijing in 2010 were analyzed in this study.

Results

A total of 140 pneumococcal isolates were collected. The resistance rates of all isolates to erythromycin and tetracycline were 96.4% and 79.3%, respectively. Of the 135 erythromycin-resistant pneumococci, 91.1% were non-susceptible to tetracycline. In addition, 30.4% of the erythromycin-resistant isolates expressed both the ermB and mef genes, whereas 69.6% expressed the ermB gene but not the mef gene. Up to 98.5% of the resistant isolates exhibited the cMLSB phenotype, and Tn6002 was the most common transposon present in approximately 56.3% of the resistant isolates, followed by Tn2010, with a proportion of 28.9%. The dominant sequence types (STs) in all erythromycin-resistant S. pneumoniae were ST271 (11.9%), ST81 (8.9%), ST876 (8.9%), and ST320 (6.7%), whereas the prevailing serotypes were 19F (19.3%), 23F (9.6%), 14 (9.6%), 15 (8.9%), and 6A (7.4%). The 7-valent pneumococcal conjugate vaccine (PCV7) and 13-valent pneumococcal conjugate vaccine (PCV13) coverage of the erythromycin-resistant pneumococci among the children younger than five years were 45.2% and 62.2%, respectively. ST320 and serotype 19A pneumococci were common in children aged 0 to 2 years. CC271 was the most frequent clonal complex (CC), which accounts for 24.4% of all erythromycin-resistant isolates.

Conclusions

The non-invasive S. pneumoniae in children younger than five years in Beijing presented high and significant resistance rates to erythromycin and tetracycline. The expressions of ermB and tetM genes were the main factors that influence pneumococcal resistance to erythromycin and tetracycline, respectively. Majority of the erythromycin-resistant non-invasive isolates exhibited the cMLSB phenotype and carried the ermB, tetM, xis, and int genes, suggesting the spread of the transposons of the Tn916 family. PCV13 provided higher serotype coverage in the childhood pneumococcal diseases caused by the erythromycin-resistant isolates better than PCV7. Further long-term surveys are required to monitor the molecular characteristics of the erythromycin-resistant S. pneumoniae in children.
Quantitative RT-PCR based platform for rapid quantification of the transcripts of highly homologous multigene families and their members during grain development
Authors: Kaczmarczyk Agnieszka , Bowra Steve , Elek Zoltan , Vincze Eva
Published: October 2012

Background

Cereal storage proteins represent one of the most important sources of protein for food and feed and they are coded by multigene families. The expression of the storage protein genes exhibits a temporal fluctuation but also a response to environmental stimuli. Analysis of temporal gene expression combined with genetic variation in large multigene families with high homology among the alleles is very challenging.

Results

We designed a rapid qRT-PCR system with the aim of characterising the variation in the expression of hordein genes families. All the known D-, C-, B-, and γ-hordein sequences coding full length open reading frames were collected from commonly available databases. Phylogenetic analysis was performed and the members of the different hordein families were classified into subfamilies. Primer sets were designed to discriminate the gene expression level of whole families, subfamilies or individual members. The specificity of the primer sets was validated before successfully applying them to a cDNA population derived from developing grains of field grownHordeum vulgarecv. Barke. The results quantify the number of moles of transcript contributed to a particular gene family and its subgroups. More over the results indicate the genotypic specific gene expression.

Conclusions

Quantitative RT-PCR with SYBR Green labelling can be a useful technique to follow gene expression levels of large gene families with highly homologues members. We showed variation in the temporal expression of genes coding for barley storage proteins. The results imply that our rapid qRT-PCR system was sensitive enough to identify the presence of alleles and their expression profiles. It can be used to check the temporal fluctuations in hordein expressions or to find differences in their response to environmental stimuli. The method could be extended for cultivar recognition as some of the sequences from the database originated from cv. Golden Promise were not expressed in the studied barley cultivar Barke although showed primer specificity with their cloned DNA sequences.
Illness Mapping: a time and cost effective method to estimate healthcare data needed to establish community-based health insurance
Authors: Binnendijk Erika , Gautham Meenakshi , Koren Ruth , Dror David M
Published: October 2012

Background

Most healthcare spending in developing countries is private out-of-pocket. One explanation for low penetration of health insurance is that poorer individuals doubt their ability to enforce insurance contracts. Community-based health insurance schemes (CBHI) are a solution, but launching CBHI requires obtaining accurate local data on morbidity, healthcare utilization and other details to inform package design and pricing. We developed the “Illness Mapping” method (IM) for data collection (faster and cheaper than household surveys).

Methods

IM is a modification of two non-interactive consensus group methods (Delphi and Nominal Group Technique) to operate as interactive methods. We elicited estimates from “Experts” in the target community on morbidity and healthcare utilization. Interaction between facilitator and experts became essential to bridge literacy constraints and to reach consensus.

The study was conducted in Gaya District, Bihar (India) during April-June 2010. The intervention included the IM and a household survey (HHS). IM included 18 women’s and 17 men’s groups. The HHS was conducted in 50 villages with1,000 randomly selected households (6,656 individuals).

Results

We found good agreement between the two methods on overall prevalence of illness (IM: 25.9% ±3.6; HHS: 31.4%) and on prevalence of acute (IM: 76.9%; HHS: 69.2%) and chronic illnesses (IM: 20.1%; HHS: 16.6%). We also found good agreement on incidence of deliveries (IM: 3.9% ±0.4; HHS: 3.9%), and on hospital deliveries (IM: 61.0%. ± 5.4; HHS: 51.4%). For hospitalizations, we obtained a lower estimate from the IM (1.1%) than from the HHS (2.6%). The IM required less time and less person-power than a household survey, which translate into reduced costs.

Conclusions

We have shown that our Illness Mapping method can be carried out at lower financial and human cost for sourcing essential local data, at acceptably accurate levels. In view of the good fit of results obtained, we assume that the method could work elsewhere as well.
Social interactions of eating behaviour among high school students: a cellular automata approach
Authors: Dabbaghian Vahid , Mago Vijay K , Wu Tiankuang , Fritz Charles , Alimadad Azadeh
Published: October 2012

Background

Overweight and obesity in children and adolescents is a global epidemic posing problems for both developed and developing nations. The prevalence is particularly alarming in developed nations, such as the United States, where approximately one in three school-aged adolescents (ages 12-19) are overweight or obese. Evidence suggests that weight gain in school-aged adolescents is related to energy imbalance exacerbated by the negative aspects of the school food environment, such as presence of unhealthy food choices. While a well-established connection exists between the food environment, presently there is a lack of studies investigating the impact of the social environment and associated interactions of school-age adolescents. This paper uses a mathematical modelling approach to explore how social interactions among high school adolescents can affect their eating behaviour and food choice.

Methods

In this paper we use a Cellular Automata (CA) modelling approach to explore how social interactions among school-age adolescents can affect eating behaviour, and food choice. Our CA model integrates social influences and transition rules to simulate the way individuals would interact in a social community (e.g., school cafeteria). To replicate these social interactions, we chose the Moore neighbourhood which allows all neighbours (eights cells in a two-dimensional square lattice) to influence the central cell. Our assumption is that individuals belong to any of four states; Bring Healthy, Bring Unhealthy, Purchase Healthy, and Purchase Unhealthy, and will influence each other according to parameter settings and transition rules. Simulations were run to explore how the different states interact under varying parameter settings.

Results

This study, through simulations, illustrates that students will change their eating behaviour from unhealthy to healthy as a result of positive social and environmental influences. In general, there is one common characteristic of changes across time; students with similar eating behaviours tend to form groups, represented by distinct clusters. Transition of healthy and unhealthy eating behaviour is non-linear and a sharp change is observed around a critical point where positive and negative influences are equal.

Conclusions

Conceptualizing the social environment of individuals is a crucial step to increasing our understanding of obesogenic environments of high-school students, and moreover, the general population. Incorporating both contextual, and individual determinants found in real datasets, in our model will greatly enhance calibration of future models. Complex mathematical modelling has a potential to contribute to the way public health data is collected and analyzed.
Pro-inflammatory cytokines and bone fractures in CKD patients. An exploratory single centre study
Authors: Panuccio Vincenzo , Enia Giuseppe , Tripepi Rocco , Aliotta Roberta , Mallamaci Francesca , Tripepi Giovanni , Zoccali Carmine
Published: October 2012

Background

Pro-inflammatory cytokines play a key role in bone remodeling. Inflammation is highly prevalent in CKD-5D patients, but the relationship between pro-inflammatory cytokines and fractures in CKD-5D patients is unclear. We studied the relationship between inflammatory cytokines and incident bone fractures in a cohort of CKD-5D patients.

Methods

In 100 CKD-5D patients (66 on HD, 34 on CAPD; males:63, females:37; mean age: 61 ± 15; median dialysis vintage: 43 months) belonging to a single renal Unit, we measured at enrolment bone metabolic parameters (intact PTH, bone and total alkaline phosphatase, calcium, phosphate) and inflammatory cytokines (TNF-α, IL-6, CRP). Patients were followed-up until the first non traumatic fracture.

Results

During follow-up (median: 74 months; range 0.5 -84.0) 18 patients experienced fractures. On categorical analysis these patients compared to those without fractures had significantly higher intact PTH (median: 319 pg/ml IQ range: 95–741 vs 135 pg/ml IQ: 53–346; p = 0.04) and TNF-α levels (median: 12 pg/ml IQ: 6.4-13.4 vs 7.8 pg/ml IQ: 4.6-11; p = 0.02). Both TNF-α (HR for 5 pg/ml increase in TNF-α: 1.62 95% CI: 1.05-2.50; p = 0.03) and intact PTH (HR for 100 pg/ml increase in PTH: 1.15 95% CI: 1.04-1.27; p = 0.005) predicted bone fractures on univariate Cox’s regression analysis. In restricted (bivariate) models adjusting for previous fractures, age, sex and other risk factors both PTH and TNF-α maintained an independent association with incident fractures.

Conclusions

In our bivariate analyses TNF-α was significantly associated with incident fractures. Analyses in larger cohorts and with adequate number of events are needed to firmly establish the TNF α -fracture link emerged in the present study.
Parity, age at first birth, and risk of death from brain cancer: a population-based cohort study in Taiwan
Authors: Chiu Hui-Fen , Chen Chih-Cheng , Tsai Shang-Shyue , Ho Shu-Chen , Yang Chun-Yuh
Published: October 2012

Background

This study was undertaken to examine whether there is an association between parity and age at first birth and risk of death from brain cancer.

Methods

The study cohort consisted of 1,292,462 women who had a first and singleton childbirth between Jan. 1, 1978 and Dec. 31, 1987. We tracked each woman from the time of their first childbirth to December 31, 2009, and their vital status was ascertained by linking records with the computerized mortality database. Cox proportional hazard regression models were used to estimate the hazard ratios (HR) of death from brain cancer associated with parity and age at first birth.

Results

There were 316 brain cancer deaths during 34,980,246 person-years of follow-up. The mortality rate of brain cancer was 0.90 cases per 100,000 person-years. The adjusted HR was 1.35 (95% CI= 0.91-2.01) for women who gave birth between 21 and 25, 1.61 (95% CI=1.05-2.45) for women who gave birth after 25 years of age, respectively, when compared with women who gave birth less than 20 years. A trend of increasing risk of brain cancer was seen with increasing age at first birth. The adjusted HR were 0.73 (95% CI= 0.53-0.99) for women who had 2 children, and 0.60 (95% CI =0.43-0.83) for women with 3 or more births, respectively, when compared with women who had given birth to only 1 child. There was a significant decreasing trend in the HRs of brain cancer with increasing parity.

Conclusions

This study provides evidence that reproductive factors (parity and early age at first birth) may confer a protective effect on the risk of death from brain cancer.
A one-year observational study of all hospitalized and fatal acute poisonings in Oslo: Epidemiology, intention and follow-up
Authors: Lund Cathrine , Teige Brita , Drottning Per , Stiksrud Birgitte , Rui Tor Olav , Lyngra Marianne , Ekeberg Øivind , Jacobsen Dag , Hovda Knut Erik
Published: October 2012

Background

Up to date information on poisoning trends is important. This study reports the epidemiology of all hospitalized acute poisonings in Oslo, including mortality, follow-up referrals, and whether the introduction of over-the-counter sales of paracetamol outside pharmacies had an impact on the frequency of poisonings.

Methods

All acute poisonings of adults (≥16 years) treated at the five hospitals in Oslo from April 2008 to April 2009 were included consecutively in an observational cross-sectional multicentre study. A standardized form was completed by the treating physician, which covered the study aims. All deaths by poisoning in and outside hospitals were registered at the Institute of Forensic Medicine.

Results

There were 1065 hospital admissions of 912 individuals; 460 (50%) were male, and the median age was 36 years. The annual incidence was 2.0 per 1000. The most frequent toxic agents were ethanol (18%), benzodiazepines (15%), paracetamol (11%), and opioids (11%). Physicians classified 46% as possible or definite suicide attempts, 37% as accidental overdoses with substances of abuse (AOSA), and 16% as other accidents. Twenty-four per cent were discharged without any follow-up and the no follow-up odds were highest for AOSA. There were 117 deaths (eight in hospital), of which 75% were males, and the median age was 41 years. Thus, the annual mortality rate was 25 per 100 000 and the in-hospital mortality was 0.8%. Opioids were the most frequent cause of death.

Conclusions

The incidence of hospitalized acute poisonings in Oslo was similar to that in 2003 and there was an equal sex distribution. Compared with a study performed in Oslo in 2003, there has been an increase in poisonings with a suicidal intention. The in-hospital mortality was low and nine out of ten deaths occurred outside hospitals. Opioids were the leading cause of death, so preventive measures should be encouraged among substance abusers. The number of poisonings caused by paracetamol remained unchanged after the introduction of over-the-counter sales outside pharmacies and there were no deaths, so over-the-counter sales may be considered safe.
Association of the UCP-1 single nucleotide polymorphism A-3826G with the dampness-phlegm pattern among Korean stroke patients
Authors: Lim Ji Hye , Ko Mi Mi , Moon Tae-Woong , Cha Min Ho , Lee Myeong Soo
Published: October 2012

Background

Patients with stroke have various syndromes and symptoms. Through pattern identification (PI), traditional Korean medicine (TKM) classifies the several syndromes and symptoms of stroke patients into five categories: Fire-heat (FH), Dampness-phlegm (DP), Yin-deficiency (YD), Qi-deficiency (QD) and Blood-stasis (BS). DP has been associated with obesity and hyperlipidemia. Uncoupling protein-1 (UCP-1), which plays a major role in thermogenesis and energy expenditure can increase the risk of obesity and can be related metabolic disorders. In this study, we elucidated the association of three polymorphisms located in the UCP-1 promoter and coding region with DP among Korean stroke patients.

Methods

1,593 patients with cerebral infarction (583/DP, 1,010/non-DP) and 587 normal subjects were enrolled. The genotypes A-3826G, G-1766A and Ala64Thr (G+1068A) for each subject were determined by polymerase chain reaction with TaqMan probes and five percent of subjects were re-genotyped by sequencing method to confirm the accuracy of genotyping. The results were analyzed using a multiple logistic regression model to evaluate the genetic associations: the UCP-1polymorphisms of normal versus those of DP subjects and those of normal versus those of non-DP subjects.

Results

A significantly higher percentage of subjects in the DP group possessed the A-3826G G allele than the A allele (OR=1.508, p=0.006). Furthermore, the number of subjects with the GG type of A-1766G was significantly lower in the non-DP group than the normal group in the recessive model (OR=0.606, p=0.042). In addition, an analysis of the relationship among 2 SNPs of UCP-1 and lipid serum concentration showed that the serum level of HDL cholesterol was significantly higher in subjects with the A-3826G G allele in the normal group (p=0.032). Serum triglyceride and HDL cholesterol were also associated with the A-1766G variant in the recessive model (p=0.002, p=0.046).

Conclusions

These results suggest that that the A-3826G and A-1766G UCP-1 polymorphisms, which are related to obesity, might be candidate genetic markers for the DP pattern in the TKM diagnosis system.
Iranian cancer patients’ perception of spirituality: a qualitative content analysis study
Authors: Rahnama Mozhgan , Khoshknab Masoud Fallahi , Maddah Sadat Seyed Bagher , Ahmadi Fazlollah
Published: October 2012

Background

Spirituality is a subjective and multi-dimensional concept. The ambiguity in its meaning can create barriers in its application in both education and medicine. The present study aimed to explore the Iranian cancer patients’ perception of spirituality.

Methods

A qualitative study, using the content analysis approach, was conducted. Semi-structured interviews were held with 11 cancer patients and six members of their families in one of Tehran’s hospitals and a charity institute. The data generated were transcribed verbatim and content analysis approach was used for data reduction, naming data, obtaining analytical code and determining categories and themes.

Results

Three themes (and seven sub-themes) emerged from the data analysis: 1) God as the spiritual truth (relationship with God and trust in God), 2) Moralities as a spiritual sign (considering personal and social moral codes) and 3) Spiritual resources as the source of hope (religious, personal and social resources).

Conclusions

Overall, in the view of cancer patients, spirituality can be defined in a religious context. However, some of them believe in morality beside religiosity, so health care staff must pay due attention to these aspects, to provide them with the opportunity to use spiritual resources.
Distinguishing nanowire and nanotube formation by the deposition current transients
Authors: Proenca Mariana P , Sousa Célia T , Ventura João , Vazquez Manuel , Araujo João P
Published: October 2012

High aspect ratio Ni nanowires (NWs) and nanotubes (NTs) were electrodeposited inside ordered arrays of self-assembled pores (approximately 50 nm in diameter and approximately 50 μm in length) in anodic alumina templates by a potentiostatic method. The current transients monitored during each process allowed us to distinguish between NW and NT formation. The depositions were long enough for the deposited metal to reach the top of the template and form a continuous Ni film. The overfilling process was found to occur in two steps when depositing NWs and in a single step in the case of NTs. A comparative study of the morphological, structural, and magnetic properties of the Ni NWs and NTs was performed using scanning electron microscopy, X-ray diffraction, and vibrating sample magnetometry, respectively.
Coupled fixed and coincidence points for monotone operators in partial metric spaces
Authors: Alsulami Saud M , Hussain Nawab , Alotaibi Abdullah
Published: October 2012

In this paper, we prove some coupled fixed point results for

(
ϕ
,
φ
)

-weakly contractive mappings in ordered partial metric spaces. As an application, we establish coupled coincidence results without any type of commutativity of the concerned maps. Consequently, the results of Luong and Thuan (Nonlinear Anal. 74:983-992, 2011), Alotaibi and Alsulami (Fixed Point Theory Appl. 2011:44, 2011) and many others are extended to the class of ordered partial metric spaces.
Impact of intravenous immunoglobulin on the dopaminergic system and immune response in the acute MPTP mouse model of Parkinson’s disease
Authors: St-Amour Isabelle , Bousquet Mélanie , Paré Isabelle , Drouin-Ouellet Janelle , Cicchetti Francesca , Bazin Renée , Calon Frédéric
Published: October 2012

Intravenous immunoglobulin (IVIg) is a blood-derived product, used for the treatment of immunodeficiency and autoimmune diseases. Since a range of immunotherapies have recently been proposed as a therapeutic strategy for Parkinson’s disease (PD), we investigated the effects of an IVIg treatment in a neurotoxin-induced animal model of PD. Mice received four injections of MPTP (15 mg/kg) at 2-hour intervals followed by a 14-day IVIg treatment, which induced key immune-related changes such as increased regulatory T-cell population and decreased CD4+/CD8+ratio. The MPTP treatment induced significant 80% and 84% decreases of striatal dopamine concentrations (P < 0.01), as well as 33% and 40% reductions in the number of nigral dopaminergic neurons (P < 0.001) in controls and IVIg-treated mice, respectively. Two-way analyses of variance further revealed lower striatal tyrosine hydroxylase protein levels, striatal homovanillic acid concentrations and nigral dopaminergic neurons (P < 0.05) in IVIg-treated animals. Collectively, our results fail to support a neurorestorative effect of IVIg on the nigrostriatal system in the MPTP-treated mice and even suggest a trend toward a detrimental effect of IVIg on the dopaminergic system. These preclinical data underscore the need to proceed with caution before initiating clinical trials of IVIg in PD patients.
The efficacy of a brief intervention to reduce alcohol misuse in patients with HIV in South Africa: study protocol for a randomized controlled trial
Authors: Huis in ’t Veld Diana , Skaal Linda , Peltzer Karl , Colebunders Robert , Ndimande John V , Pengpid Supa
Published: October 2012

Background

Alcohol abuse comes with risks for increased morbidity and mortality among patients with HIV. This study aims to determine the prevalence of alcohol use and other risk factors in a sample of primary care patients with HIV in South Africa and to assess a brief intervention to reduce the use of alcohol in this group.

Methods/Design

A single-blinded randomized controlled trial is designed to determine the efficacy of a brief intervention to reduce hazardous alcohol use in patients with HIV. The study will be carried out on out-patients with HIV in two primary healthcare HIV clinics near Pretoria, South Africa. Alcohol use will be assessed with the Alcohol Use Disorder Identification Test questionnaire. Other data that will be collected relate to health-related quality of life, depression, sexual behavior, internalized AIDS stigma, HIV-related information and adherence to antiretroviral therapy (self-reported 7-day recall of missed doses, Visual Analog Scale and pill count). The intervention consists of a brief counseling session to reduce alcohol risk; the control group receives a health education leaflet.

Discussion

The findings will be important in the public health setting. If the intervention proves to be efficient, it could potentially be incorporated into the HIV care policy of the Ministry of Health.

Trial registration

Pan African Clinical trial Registry: PACTR201202000355384
Pseudomyotonia in Romagnola cattle caused by novel ATP2A1mutations
Authors: Murgiano Leonardo , Sacchetto Roberta , Testoni Stefania , Dorotea Tiziano , Mascarello Francesco , Liguori Rocco , Gentile Arcangelo , Drögemüller Cord
Published: October 2012

Background

Bovine congenital pseudomyotonia (PMT) is an impairment of muscle relaxation induced by exercise preventing animals from performing rapid movements. Forms of recessively inherited PMT have been described in different cattle breeds caused by two independent mutations in ATP2A1 encoding a skeletal-muscle Ca2+-ATPase (SERCA1). We observed symptoms of congenital PMT in four related Romagnola beef cattle from Italy and evaluated SERCA1 activity and scanned ATP2A1 for possible causative mutations.

Results

We obtained four PMT affected Romagnola cattle and noted striking clinical similarities to the previously described PMT cases in other cattle breeds. The affected animals had a reduced SERCA1 activity in the sarcoplasmic reticulum. A single affected animal was homozygous for a novel complex variant in ATP2A1 exon 8 (c.[632 G>T; 857 G>T]). Three out of four cases were compound heterozygous for the newly identified exon 8 variant and the exon 6 variant c.491 G>A(p. Arg146Gly), which has previously been shown to cause PMT in Chianina cattle. Pedigree analysis showed that the exon 8 double mutation event dates back to at least 1978. Both nucleotide substitutions are predicted to alter the SERCA1 amino acid sequence (p.[(Gly211Val; Gly284Val)]), affect highly conserved residues, in particular the actuator domain of SERCA1.

Conclusion

Clinical, biochemical and DNA analyses confirmed the initial hypothesis. We provide functional and genetic evidence that one novel and one previously described ATP2A1 mutation lead to a reduced SERCA1 activity in skeletal muscles and pseudomyotonia in affected Romagnola cattle. Selection against these mutations can now be used to eliminate the mutant alleles from the Romagnola breed.
Arsenic trioxide improves hematopoiesis in refractory severe aplastic anemia
Authors: Li Ning , Song Yongping , Zhou Jian , Fang Baijun
Published: October 2012

We investigated the efficacy of arsenic trioxide (ATO) in patients with refractory severe aplastic anemia (SAA). A total of 5 consecutive adults were enrolled. The patients received ATO at a dose of 0.15 mg/kg intravenously daily for 5 days every week for 8 weeks. If necessary, a second course was performed after an interval of one week. All patients achieved clinically significant responses to ATO. The overall complete response rate and overall response rate at 17 weeks were 60% (3/5) and 100%(5/5), respectively. So treatment with ATO may be a feasible approach in patients with refractory SAA.
What happens after enrollment? An analysis of the time path of racial differences in GPA and major choice
Authors: Arcidiacono Peter , Aucejo Esteban M , Spenner Ken
Published: October 2012

At the private university we analyze, the gap between white and black grade point averages falls by half between the students' freshmen and senior year. This outcome could suggest that affirmative action policies are playing a key role to reduce racial differences. However, this convergence masks two effects. First, the variance of grades given falls across time. Hence, shrinkage in the level of the gap may not imply shrinkage in the class rank gap. Second, grading standards differ across courses in different majors. We show that controlling for these two features virtually eliminates any convergence of black/white grades. In fact, black/white gpa convergence is symptomatic of dramatic shifts by blacks from initial interest in the natural sciences, engineering, and economics to majors in the humanities and social sciences. We show that natural science, engineering, and economics courses are more difficult, associated with higher study times, and have harsher grading standards; all of which translate into students with weaker academic backgrounds being less likely to choose these majors. Indeed, we show that accounting for academic background can fully account for average differences in switching behavior between blacks and whites.
Immigration, obesity and labor market outcomes in the UK
Authors: Averett Susan L , Argys Laura M , Kohn Jennifer L
Published: October 2012

We estimate the dual effects of immigration and obesity on labor market outcomes in the UK using the British Household Panel Survey. We find support for the “healthy immigrant hypothesis” and evidence that immigrants’ weights increase with time in the UK. While overweight and obese men enjoy a wage premium, overweight and obese immigrant men face a wage penalty and are less likely to work in a white collar job. Overweight immigrant women are substantially more likely to suffer work limitations. While data limitations preclude efforts to address endogeneity, these associations suggest that immigrants have not been spared from the obesity epidemic.
Carob seed oil: an efficient inhibitor of C38 steel corrosion in hydrochloric acid
Authors: Hmamou Dris Ben , Salghi Rachid , Zarrouk Abdelkader , Benali Omar , Fadel Fatiha , Zarrok Hassan , Hammouti Belkheir
Published: October 2012

Background

The carob seed oil (CO) was tested as inhibitor of the corrosion of C38 steel in 1 M HCl by weight loss and electrochemical measurements. The extract was found to inhibit the corrosion of C38 steel in 1 M HCl.

Results

The results of the study reveal that the inhibition efficiency of CO depends on its concentration and attains approximately 86.7% at 0.5 g/L. Polarization curves reveal that CO is a mixed-type inhibitor. Changes in impedance parameters (charge transfer resistance,Rt, and double-layer capacitance,Cdl) were indicative of CO adsorption on the metal surface, leading to the formation of a protective film. The effect of temperature on the corrosion behavior with the addition of 0.5 g/L of carob seed oil was studied in the temperature range of 298 to 328 K.

Conclusions

Results show that the inhibition efficiency of the plant extract increases with increasing temperature, and the adsorption of the latter on the C38 steel surface is found to obey the Langmuir adsorption isotherm. Some thermodynamic functions of dissolution processes were also determined.
An analysis on classifications of hyperbolic and elliptic PDEs
Authors: Eltayeb Hassan , Kılıçman Adem , Agarwal Ravi P
Published: October 2012

Purpose

Our aim in this study is to generate some partial differential equations (PDEs) with variable coefficients by using the PDEs with non-constant coefficients.

Methods

Then by applying the single and double convolution products, we produce some new equations having polynomials coefficients. We then classify the new equations on using the classification method for the second order linear partial differential equations.

Results

Classification is invariant under single and double convolutions by applying some conditions, that is, we identify some conditions where a hyperbolic equation will be hyperbolic again after single and double convolutions.

Conclusions

It is shown that the classifications of the new PDEs are related to the coefficients of polynomials which are considered during the process of convolution product.