bluuebunny/crossref_metadata_2025 huggingface dataset:
Sucrose Consumption Alters Serotonin/Glutamate Co-localisation Within the Prefrontal Cortex and Hippocampus of Mice
Authors: Beecher Kate , Wang Joshua , Jacques Angela , Chaaya Nicholas , Chehrehasa Fatemeh , Belmer Arnauld , Bartlett Selena E.
Published: June 2021

The overconsumption of sugar-sweetened food and beverages underpins the current rise in obesity rates. Sugar overconsumption induces maladaptive neuroplasticity to decrease dietary control. Although serotonin and glutamate co-localisation has been implicated in reward processing, it is still unknown how chronic sucrose consumption changes this transmission in regions associated with executive control over feeding—such as the prefrontal cortex (PFC) and dentate gyrus (DG) of the hippocampus. To address this, a total of 16 C57Bl6 mice received either 5% w/v sucrose or water as a control for 12 weeks using the Drinking-In-The-Dark paradigm (n = 8 mice per group). We then examined the effects of chronic sucrose consumption on the immunological distribution of serotonin (5-HT), vesicular glutamate transporter 3 (VGLUT3) and 5-HT+/VGLUT3+ co-localised axonal varicosities. Sucrose consumption over 12 weeks decreased the number of 5-HT–/VGLUT3+ and 5-HT+/VGLUT3+ varicosities within the PFC and DG. The number of 5-HT+/VGLUT3– varicosities remained unchanged within the PFC but decreased in the DG following sucrose consumption. Given that serotonin mediates DG neurogenesis through microglial migration, the number of microglia within the DG was also assessed in both experimental groups. Sucrose consumption decreased the number of DG microglia. Although the DG and PFC are associated with executive control over rewarding activities and emotional memory formation, we did not detect a subsequent change in DG neurogenesis or anxiety-like behaviour or depressive-like behaviour. Overall, these findings suggest that the chronic consumption of sugar alters serotonergic neuroplasticity within neural circuits responsible for feeding control. Although these alterations alone were not sufficient to induce changes in neurogenesis or behaviour, it is proposed that the sucrose consumption may predispose individuals to these cognitive deficits which ultimately promote further sugar intake.
Treating Diabetes Utilizing a Low Carbohydrate Ketogenic Diet and Intermittent Fasting Without Significant Weight Loss: A Case Report
Authors: Gavidia Kristen , Kalayjian Tro
Published: June 2021

Prediabetes and diabetes are leading causes of morbidity and mortality in the United States and are growing in prevalence up to 45% of the population over the past 50 years. Current guidelines from the ADA recommend focusing on energy balance, portion sizes, and weight loss while cautioning that no ideal macronutrient composition has been determined. The guidelines also do not recommend intermittent fasting. In contrast, we report three cases of a substantial reduction in A1C without clinically significant weight loss using a unique, patient-centered program that utilizes low carbohydrate diets with intermittent fasting. These results call into question the role of weight reduction in the management of diabetes while highlighting the unique importance of carbohydrate restriction and intermittent fasting. In this study, we demonstrate a case series of three patients with a substantial reduction in A1C and significantly reducing the need for pharmacotherapy without clinically significant weight loss. Although anecdotal, these results call into question the emphasis of ADA on weight reduction and energy intake reduction for the management of diabetes.
A Novel HAGE/WT1-ImmunoBody® Vaccine Combination Enhances Anti-Tumour Responses When Compared to Either Vaccine Alone
Authors: Almshayakhchi Rukaia , Nagarajan Divya , Vadakekolathu Jayakumar , Guinn Barbara-Ann , Reeder Stephen , Brentville Victoria , Metheringham Rachael , Pockley A. Graham , Durrant Lindy , McArdle Stephanie
Published: June 2021

Many cancers, including myeloid leukaemia express the cancer testis antigen (CTA) DDX43 (HAGE) and/or the oncogene Wilms’ tumour (WT1). Here we demonstrate that HAGE/WT1-ImmunoBody®vaccines derived T-cells can killex-vivohuman CML cell lines expressing these antigens and significantly delay B16/HHDII+/DR1+/HAGE+/WT1+tumour growth in the HHDII/DR1 mice and prolonged mouse survival in the prophylactic setting in comparison to non-immunised control mice. We show that immunisation of HHDII/DR1 mice with HAGE- and WT1-ImmunoBody®DNA vaccines in a prime-boost regime in two different flanks induce significant IFN-γ release by splenocytes from treated mice, and a significant level of cytotoxicity against tumour targets expressing HAGE/WT1in vitro. More importantly, the combined HAGE/WT1 ImmunoBody®vaccine significantly delayed tumour growth in the B16/HHDII+/DR1+/HAGE+/WT1+tumour model and prolonged mouse survival in the prophylactic setting in comparison to non-immunised control mice. Overall, this work demonstrates that combining both HAGE- and WT1-ImmunoBody®into a single vaccine is better than either vaccine alone. This combination vaccine could be given to patients whose cancer expresses HAGE and WT1 in parallel with existing therapies in order to decrease the chance of disease progression and relapse.
Whole-Genome Methylation Study of Congenital Lung Malformations in Children
Authors: Patrizi Sara , Pederiva Federica , d’Adamo Adamo Pio
Published: June 2021

Background and Objectives

The treatment of asymptomatic patients with congenital pulmonary malformations (CPMs) remains controversial, partially because the relationship between congenital lung malformations and malignancy is still undefined. Change in methylation pattern is a crucial event in human cancer, including lung cancer. We therefore studied all differentially methylated regions (DMRs) in a series of CPMs in an attempt to find methylation anomalies in genes already described in association with malignancy.

Methods

The DNA extracted from resected congenital lung malformations and control lung tissue was screened using Illumina MethylationEPIC arrays. Comparisons between the group of malformed samples or the malformed samples of same histology or each malformed sample and the controls and between a pleuropulmonary blastoma (PPB) and controls were performed. Moreover, each malformed sample was pairwise compared with its respective control. All differentially methylated regions (DMRs) with an adjusted p-value <0,05 were studied.

Results

Every comparison highlighted a number of DMRs closed to genes involved either in cell proliferation or in embryonic development or included in the Cancer Gene Census. Their abnormal methylation had been already described in lung tumors.

Conclusions

Methylation anomalies already described in lung tumors and also shared by the PPB were found in congenital lung malformations, regardless the histology. The presence of methylation abnormalities is suggestive of a correlation between congenital lung malformations and some step of malignant transformation.
Pembrolizumab Alone or Combined With Chemotherapy in Advanced NSCLC With PD-L1 ≥50%: Results of a Retrospective Study
Authors: Chen Ya , Wang Yanan , Yang Zhengyu , Hu Minjuan , Zhang Yanwei , Qian Fangfei , Zhang Wei , Zhang Bo , Han Baohui
Published: June 2021

Objectives

Pembrolizumab plus platinum-based chemotherapy and pembrolizumab monotherapy (PM) both become standard of care in patients with advanced non-small-cell lung cancer (NSCLC) and a programmed death ligand 1 (PD-L1) tumor proportion score (TPS) greater than 50%. This study aimed to figure out the better treatment choice.

Method

In this retrospective analysis, we compared the clinical efficacy of PM and PC as first-line treatment in NSCLC patients with a PD-L1 ≥50% and negative for genomic alterations in the EGFR and ALK genes.

Result

Among the population, 115 patients received PC, and 91 patients received PM. Up to Dec 30, 2020, median follow-up was 17.13 months. The median progression-free survival (PFS) rates of PC and PM were 12.37 and 9.60 months (HR: 0.44, p < 0.001), respectively. The median overall survival (OS) rates were NE and 28.91 months (HR: 0.40, p = 0.005), respectively. Subgroup analysis found that the PFS benefit of PC was evident in most subgroups excepting patients with brain metastasis. The 1-year overall survival rates of PC and PM were 89.3% and 76.1%, respectively. The ORR was 61.7 and 46.9% (p = 0.004), respectively.

Conclusion

In patients with previously untreated, PD-L1 ≥50%, advanced NSCLC without EGFR or ALK mutations, the addition of pembrolizumab to standard platinum-based chemotherapy seems to be the preferred treatment, which needs to be validated by further prospective trials.
Colonoscopic Diagnosis of Postoperative Gastrointestinal Bleeding in Patients With Hirschsprung's Disease
Authors: Yang Jixin , Zhu Tianqi , Wu Xiaojuan , Wei Mingfa , Wang Guo , Feng Jiexiong
Published: June 2021

Aim: Postoperative lower gastrointestinal bleeding in children with Hirschsprung's Disease (HSCR) is a non-specific symptom, which may be caused by various etiologies. Our current study aims to utilize colonoscopy to diagnose the causes of postoperative hematochezia and to analyze its feasibility, accuracy, and safety.

Methods: Twenty-four patients with HSCR with postoperative lower gastrointestinal bleeding or occult blood in the stool were enrolled in this study. The postoperative onset duration, age at examination, accompanied anomalies were recorded. After bowel preparation, all patients underwent colonoscopy. According to visual findings, mucosal biopsy was performed, followed by pathological diagnosis. Further treatment was determined according to the visual findings and pathological diagnosis. All patients were followed up for 6 months including therapeutic outcomes and recurrence of symptoms.

Results: The mean onset duration was (221.3 ± 216.8) days postoperatively (ranging from 25 to 768 days). The mean age at examination was (41.0 ± 29.4) months. There was no significant difference in the onset days among each group (all, p > 0.05). Based on visual and pathological findings, there were 11 cases of HSCR associated enterocolitis (HAEC), 4 cases of anastomotic leakage, 7 cases of anastomotic inflammation, 1 case of juvenile polyp, and 1 case of inflammatory pseudopolyp. Intraluminal saline irrigation, thrombin treatment or colorectal polyp electrocision was performed according to intraoperative diagnosis. Patients with HEAC and anastomotic inflammation underwent antibiotics therapy and colorectal irrigation. Patients with leakage underwent reoperation. The highest incidence of accompanied symptoms of diarrhea existed in HEAC group (p = 0.02) and fever in leakage group (p = 0.02), respectively. No perforation or aggravated bleeding occurs in any patients. All patients gained uneventful recovery during follow-up period.

Conclusions: Colonoscopy is a safe, accurate and timely examination for HSCR patients with postoperative lower gastrointestinal bleeding. The visual findings and biopsy may provide accurate diagnosis and guide treatment for this subset of patients.
Hochuekkito can Prevent the Colonization of Methicillin-Resistant Staphylococcus aureus in Upper Respiratory Tract of Acute Stroke Patients
Authors: Kitahara Masakazu , Takayama Shin , Akaishi Tetsuya , Kikuchi Akiko , Ishii Tadashi
Published: June 2021

Background: Methicillin-resistant Staphylococcus aureus (MRSA) colonization can lead to MRSA pneumonia or other infections in compromised hosts, and invasive MRSA infections lead to significant morbidity and mortality. The present observational study elucidated whether administration of hochuekkito (HET) can prevent MRSA colonization in the upper respiratory tract and support recovery in acute stroke patients.

Methods: In this retrospective, observational study, 73 acute stroke patients admitted to Kenwakai Hospital between April 2007 and December 2019 who did not require emergency surgery during this period were enrolled. Conventional treatment was provided to all patients, depending on their condition, and 7.5 g/day of HET was administered to the patients who could take the medicine via nasogastric tube or orally in three divided doses for three months. Bacterial cultures from laryngeal swabs and sputum were evaluated every week. We evaluated the presence of MRSA infection or another infectious disease within 30 days of admission; modified Rankin Scale scores, which assesses the independent living skills after stroke at three months after admission; and blood biomarkers (white blood cell count, albumin levels, C-reactive protein levels, and hemoglobin levels).

Results: In total, 73 patients (HET group, n = 41; non-HET group, n = 32) were enrolled in the study. MRSA detection was significantly less likely in the HET group than in the non-HET group (p = 0.0497). The incidence of infectious diseases was significantly lower in the HET group than in the non-HET group (p = 0.0096), and the modified Rankin Scale score at three months was also significantly lower in the HET group than in the non-HET group (p = 0.033). The white blood cell count, and serum C-reactive protein levels did not differ between those who were treated with HET and those who were not. However, serum albumin and hemoglobin levels improved slightly between month one and month three after admission only in those who were treated with HET.

Conclusion: Our results indicate that the administration of HET may contribute to the prevention of MRSA colonization and promote rehabilitation in stroke patients.
The Study on the Mechanism of Hugan Tablets in Treating Drug-Induced Liver Injury Induced by Atorvastatin
Authors: Lv Shujing , Yu Honghong , Liu Xinyue , Gao Xiaoyan
Published: June 2021

Atorvastatin is a widely used lipid-lowering drug in the clinic. Research shows that taking long-term atorvastatin has the risk of drug-induced liver injury (DILI) in most patients. Hugan tablets, a commonly used drug for liver disease, can effectively lower transaminase and protect the liver. However, the underlying mechanism of Hugan tablets alleviating atorvastatin-induced DILI remains unclear. To address this problem, comprehensive chemical profiling and network pharmacology methods were used in the study. First, the strategy of “compound−single herb−TCM prescription” was applied to characterize the ingredients of Hugan tablets. Then, active ingredients and potential targets of Hugan tablets in DILI treatment were screened using network pharmacology, molecular docking, and literature research. In the end, the mechanism of Hugan tablets in treating atorvastatin-induced DILI was elucidated. The results showed that Hugan tablets can effectively alleviate DILI induced by atorvastatin in model rats, and 71 compounds were characterized from Hugan tablets. Based on these compounds, 271 potential targets for the treatment of DILI were predicted, and 10 key targets were chosen by characterizing protein–protein interactions. Then, 30 potential active ingredients were screened through the molecular docking with these 10 key targets, and their biological activity was explained based on literature research. Finally, the major 19 active ingredients of Hugan tablets were discovered. In addition, further enrichment analysis of 271 targets indicated that the PI3K-Akt, TNF, HIF-1, Rap1, and FoxO signaling pathways may be the primary pathways regulated by Hugan tablets in treating DILI. This study proved that Hugan tablets could alleviate atorvastatin-induced DILI through multiple components, targets, and pathways.
Increased Participation and Decreased Performance in Recreational Master Athletes in “Berlin Marathon” 1974–2019
Authors: Reusser Marlen , Sousa Caio Victor , Villiger Elias , Alvero Cruz José Ramón , Hill Lee , Rosemann Thomas , Nikolaidis Pantelis T. , Knechtle Beat
Published: June 2021

The aspect of participation and performance trends in marathon running has been investigated mainly in marathons held in the United States of America (e.g., “New York City Marathon,” “Boston Marathon”), but not for the fastest course in the world, the “Berlin Marathon” held in Berlin, Germany. This study aimed to examine trends in participation and performance in the “Berlin Marathon” on all its previous 46 editions from 1974 to 2019, the largest dataset ever studied in this event with 696,225 finishers (after data cleaning). Athletes in all age groups increased their participation, except for male athletes aged 20–49 years and athletes of both sexes above 79 years of age. This overall increase in participation was more pronounced in women, but still, there are more men than women participating in “Berlin Marathon” nowadays. All age group athletes decreased their performance across years overall, whereas the top ten recreational athletes improved their performance over the years. Our findings improved the knowledge about the evolution of male and female marathoners across calendar years, especially for the fastest marathon race in the world, the “Berlin Marathon.”
The Long-Term Effects of Developmental Hypoxia on Cardiac Mitochondrial Function in Snapping Turtles
Authors: Galli Gina L. J. , Ruhr Ilan M. , Crossley Janna , Crossley Dane A.
Published: June 2021

It is well established that adult vertebrates acclimatizing to hypoxic environments undergo mitochondrial remodeling to enhance oxygen delivery, maintain ATP, and limit oxidative stress. However, many vertebrates also encounter oxygen deprivation during embryonic development. The effects of developmental hypoxia on mitochondrial function are likely to be more profound, because environmental stress during early life can permanently alter cellular physiology and morphology. To this end, we investigated the long-term effects of developmental hypoxia on mitochondrial function in a species that regularly encounters hypoxia during development—the common snapping turtle (Chelydra serpentina). Turtle eggs were incubated in 21% or 10% oxygen from 20% of embryonic development until hatching, and both cohorts were subsequently reared in 21% oxygen for 8 months. Ventricular mitochondria were isolated, and mitochondrial respiration and reactive oxygen species (ROS) production were measured with a microrespirometer. Compared to normoxic controls, juvenile turtles from hypoxic incubations had lower Leak respiration, higher P:O ratios, and reduced rates of ROS production. Interestingly, these same attributes occur in adult vertebrates that acclimatize to hypoxia. We speculate that these adjustments might improve mitochondrial hypoxia tolerance, which would be beneficial for turtles during breath-hold diving and overwintering in anoxic environments.
Fine Mapping and Candidate Gene Identification for the CapUp Locus Controlling Fruit Orientation in Pepper (Capsicum spp.)
Authors: Solomon Abate Mekonnen , Kim Tae-Gun , Han Koeun , Lee Hea-Young , Patil Abhinandan , Siddique Muhammad Irfan , Ahn Jeonghwan , Kang Byoung-Cheorl
Published: June 2021

The orientation of fruits is a distinguishing morphological feature of pepper (Capsicumspp.) varieties. The pendent (downward curved) growth of the fruit stalks, known as pedicels, is highly correlated with fruit weight and pedicel length. A previous genetic analysis revealed that the pendent fruit orientation is governed by a dominant gene, and incomplete inheritance is also observed in someCapsicumaccessions. To identify and localize this gene, a single quantitative trait locus (QTL) analysis was performed on one F2and two recombinant inbred line (RIL) populations, and a genome-wide association study (GWAS) was performed using a core collection. Common QTL regions associated with fruit orientation were detected on chromosome 12. A total of 187,966 SNPs were identified in a genotyping-by-sequencing (GBS) for GWAS analysis of 196Capsicum annuum, 25Capsicum baccatum, 21Capsicum chinense, and 14Capsicum frutescensaccessions, representing the germplasm collection of South Korea. The results of these analyses enabled us to narrow down theCapUpregion of interest to 200–250 Mbp on chromosome 12. Seven candidate genes were found to be located between two markers that were completely cosegregated with the fruit orientation phenotype. The findings and markers developed in this study will be helpful for additional understanding of pepper fruit development and breeding for fruit orientation.
Melatonin Improves Cotton Salt Tolerance by Regulating ROS Scavenging System and Ca2 + Signal Transduction
Authors: Zhang Yuexin , Fan Yapeng , Rui Cun , Zhang Hong , Xu Nan , Dai Maohua , Chen Xiugui , Lu Xuke , Wang Delong , Wang Junjuan , Wang Jing , Wang Qinqin , Wang Shuai , Chen Chao , Guo Lixue , Zhao Lanjie , Ye Wuwei
Published: June 2021

As one of the cash crops, cotton is facing the threat of abiotic stress during its growth and development. It has been reported that melatonin is involved in plant defense against salt stress, but whether melatonin can improve cotton salt tolerance and its molecular mechanism remain unclear. We investigated the role of melatonin in cotton salt tolerance by silencing melatonin synthesis gene and exogenous melatonin application in upland cotton. In this study, applicating of melatonin can improve salt tolerance of cotton seedlings. The content of endogenous melatonin was different in cotton varieties with different salt tolerance. The inhibition of melatonin biosynthesis related genes and endogenous melatonin content in cotton resulted in the decrease of antioxidant enzyme activity, Ca2+ content and salt tolerance of cotton. To explore the protective mechanism of exogenous melatonin against salt stress by RNA-seq analysis. Melatonin played an important role in the resistance of cotton to salt stress, improved the salt tolerance of cotton by regulating antioxidant enzymes, transcription factors, plant hormones, signal molecules and Ca2+ signal transduction. This study proposed a regulatory network for melatonin to regulate cotton’s response to salt stress, which provided a theoretical basis for improving cotton’s salt tolerance.
Public Attitudes and Private Prejudices: Assessing Voters’ Willingness to Vote for Out Lesbian and Gay Candidates
Authors: Everitt Joanna , Horvath Laszlo
Published: June 2021

Our study concerns the factors leading to the electoral success and failure of LGBTQ candidates in the context of the changing nature of prejudices. We hypothesize that more positive views toward “respectability candidates,” as captured by familial status, has replaced explicit prejudice toward out LGBTQ candidates in societies where acceptance of sexual minorities in general has grown. In a survey experiment conducted with a sample of Canadian voters, one of the first countries to legalize marriage equality, we find suggestions that voters are more likely to reward lesbian and gay candidates who adopt heteronormative relationships (married with children vs. single) than those who do not. These patterns become more evident when we explore causal heterogeneity with controls for individual-level characteristics and attitudes that typically predict support toward lesbian and gay candidates. Here we find these predictors rewarded single lesbian and gay candidates, whereas lesbian and gay candidates with families were simply more supported across the board.
Fatalism in the Early Days of the COVID-19 Pandemic: Implications for Mitigation and Mental Health
Authors: Hayes Joseph , Clerk Laura
Published: June 2021

This research assessed fatalism toward COVID-19 and its role in behavioral intentions to support mitigation efforts (e. g., social distancing) and mental well-being. A COVID-19 fatalism measure was developed, and a messaging manipulation (fatalistic vs. optimistic vs. no message) was created to examine causal links between fatalism scores. Support for mitigation efforts and negative affect (anxiety, fear, depression, and insecurity) were measured to examine the consequences of fatalism toward COVID-19. Results showed that the fatalistic messaging condition increased fatalism whereas the optimistic message reduced it. The effects of the messaging manipulation were also apparent in the downstream measures of support for mitigation and negative affect through the mediator of fatalism toward COVID-19. Specifically, fatalism negatively predicted intentions to support mitigation. Regarding mental health, fatalism was positively associated with depression but negatively associated with fear and insecurity. Implications for COVID-19 mitigation efforts and mental health in the face of the coronavirus pandemic are discussed.
Unpacking the Emotional Dimension of Doctoral Supervision: Supervisors' Emotions and Emotion Regulation Strategies
Authors: Han Ye , Xu Yueting
Published: June 2021

Successful completion of a PhD is challenging for both the candidate and the supervisor. While doctoral students' emotional burdens received much attention, their supervisors' emotional experiences remain under-explored. Moreover, while teacher education research stressed the importance of teacher emotion regulation, empirical studies on doctoral supervisors' emotion regulation barely exist. The current qualitative study explored 17 computer science supervisors' emotions unfolding in doctoral supervision and their emotion regulation strategies. Semi-structured interviews revealed the supervisors' wide-ranging emotions, with their negative emotions more diverse and common than positive ones. The supervisors also regulated their emotions through multiple strategies within antecedent-focused and response-focused approaches. As one of the initial studies on doctoral supervisors' emotion and emotion regulation in their own right, the current study not only uncovers the complexity of the emotion-laden dimension of supervision, but also highlights the need for all stakeholders to attend to supervisors' psychological well-being in tandem with their students'.
The Dynamic Mechanism on Team Effectiveness in Youth Football: A Chain Mediation
Authors: Li Juan
Published: June 2021

This study aims to deepen our understanding of the relevant research on coach–athlete relationship theory, moral leadership, and team effectiveness theory, and thus explore how to maximize team performance. As such, this study adopts an input-process-output model to explore the effect of coach–athlete relationships on team effectiveness in youth football teams. Participants in this anonymous survey included 312 young athletes, aged 13–19, from professional football schools who filled in questionnaires to provide data on the coach’s moral leadership, team effectiveness, coach–athlete relationships, and trust in the coach. The results indicate that coach–athlete relationships have a significant predictive effect on the moral leadership of coaches, which in turn, has a significant positive correlation with athletes’ trust in coaches; however, coach–athlete relationships have no direct positive correlation with team effectiveness. The coaches’ moral leadership and athletes’ trust in coaches have a chain mediation effect in the impact of coach–athlete relationships on team effectiveness. This study validates the assertion that coach–athlete relationships have a substantial effect on coach leadership. It also refines the coach–athlete relationship theory, provides evidence on the dynamic mechanism in which coach–athlete relationships affect team effectiveness, and enriches team effectiveness theory.
Effects of Wearing Face Masks While Using Different Speaking Styles in Noise on Speech Intelligibility During the COVID-19 Pandemic
Authors: Yi Hoyoung , Pingsterhaus Ashly , Song Woonyoung
Published: June 2021

The coronavirus pandemic has resulted in the recommended/required use of face masks in public. The use of a face mask compromises communication, especially in the presence of competing noise. It is crucial to measure the potential effects of wearing face masks on speech intelligibility in noisy environments where excessive background noise can create communication challenges. The effects of wearing transparent face masks and using clear speech to facilitate better verbal communication were evaluated in this study. We evaluated listener word identification scores in the following four conditions: (1) type of mask condition (i.e., no mask, transparent mask, and disposable face mask), (2) presentation mode (i.e., auditory only and audiovisual), (3) speaking style (i.e., conversational speech and clear speech), and (4) with two types of background noise (i.e., speech shaped noise and four-talker babble at −5 signal-to-noise ratio). Results indicate that in the presence of noise, listeners performed less well when the speaker wore a disposable face mask or a transparent mask compared to wearing no mask. Listeners correctly identified more words in the audiovisual presentation when listening to clear speech. Results indicate the combination of face masks and the presence of background noise negatively impact speech intelligibility for listeners. Transparent masks facilitate the ability to understand target sentences by providing visual information. Use of clear speech was shown to alleviate challenging communication situations including compensating for a lack of visual cues and reduced acoustic signals.
Use of Acute Psychiatric Hospitalisation: A Study of the Factors Influencing Decisions to Arrange Acute Admission to Inpatient Mental Health Facilities
Authors: Nathan Rajan , Gabbay Mark , Boyle Sean , Elliott Phil , Giebel Clarissa , O'Loughlin Carl , Wilson Pete , Saini Pooja
Published: June 2021

Background: Human decision-making involves a complex interplay of intra- and inter-personal factors. The decisions clinicians make in practise are subject to a wide range of influences. Admission to a psychiatric hospital is a major clinical intervention, but the decision-making processes involved in admissions remain unclear.

Aims: To delineate the range of factors influencing clinicians' decisions to arrange acute psychiatric admissions.

Methods: We undertook six focus groups with teams centrally involved in decisions to admit patients to hospital (crisis resolution home treatment, liaison psychiatry, approved mental health professionals and consultant psychiatrists). The data were analysed using qualitative thematic analysis.

Results: Our analysis of the data show a complex range of factors influencing decision-making that were categorised as those related to: (i) clinical and risk factors; (ii) fear/threat factors; (iii) interpersonal dynamics; (iv) contextual factors.

Conclusions: Decisions to arrange acute admission to hospital are not just based on an appraisal of clinical and risk-related information. Emotional, interpersonal and contextual factors are also critical in decision-making. Delineating the breadth of factors that bear on clinical decision-making can inform approaches to (i) clinical decision-making research, (ii) the training and supervision of clinicians, and (iii) service delivery models.
A Novel Magnetosphere–Ionosphere Coupling Model for the Onset of Substrom Expansion Phase
Authors: Wang P. S. , Lyu L. H.
Published: June 2021

A novel magnetosphere–ionosphere (M-I) coupling model is proposed to simulate the brightening of the onset auroral arc of a magnetospheric substorm event. The new M-I coupling model is modified from the M-I coupling model proposed by the Alaska research team in 1988. We adjust the magnetospheric boundary conditions by including the Hall effects in the thin current sheet and allowing the spatial distributions of the reflection–transmission coefficient to vary with time. As a result, brightening and poleward drifting of multiple auroral arcs appear for the first time in an M-I coupling model. The new results indicate that the coupled Hall effects in the near-Earth plasma sheet and the E-region ionosphere play a vital role in triggering the onset of a magnetospheric substorm.
The Usefulness of Intraoperative PTH as a Predictor for Successful Parathyroidectomy in Secondary Hyperparathyroidism
Authors: Chávez Karla Verónica , Márquez-González Horacio , Chavez-Tostado Mariana
Published: June 2021

Introduction: Secondary hyperparathyroidism (SHPT) is a multisystemic syndrome that affects calcium and bone homeostasis in patients with chronic kidney disease (CKD). Despite medical treatment, 1–2% of patients require parathyroidectomy annually. The use of an intraoperative parathormone protocol (IOPTH) to predict cure is still in debate, due to the lack of standardized protocols, the use of different assays, and uneven PTH clearance. This study aimed to determine the diagnostic accuracy of an IOPTH in patients with SHPT for predicting successful surgery after parathyroidectomy.

Methods: About 30 patients were enrolled. A prospective observational study (cohort) was performed in patients who were submitted to parathyroidectomy by an endocrine surgeon for SHPT. All were submitted to a bilateral neck exploration with a subtotal parathyroidectomy. Three IOPTH determinations were withdrawn: at anesthetic induction (PTH0), 15 min (PTH15), and 30 min (PTH30) after completion of gland resection. Another sample was taken 24 h after the procedure (PTH24), values <150 pg/mL defined a successful surgery, and patients were assigned to the success or failure group. IOPTH drop was analyzed to predict successful surgery with drops of 70 and 90% at 15 and 30 min, respectively.

Results: A total of 26 patients were included, 19 patients were in the successful group. IOPTH showed a significant difference between groups in their absolute PTH15 and PTH30 values. A significant difference was also found in their PTH drop at 30 min (81 vs. 91%, p = 0.08). For predicting a successful surgery, having a PTH drop >90% at 30 min was the most significant factor [Odds Ratio (OR) 3.0 (1.5–4) IC 95%].

Conclusions: This study points toward a stricter and staggered IOPTH protocol to predict a successful surgery. Our results suggest taking a PTH15 expecting a PTH drop of >90%. If this is not achieved, reexploration and a PTH30 sample are suggested to accurately predict success.
Abdominal Ultrasound and Its Diagnostic Accuracy in Diagnosing Acute Appendicitis: A Meta-Analysis
Authors: Fu Jian , Zhou Xu , Chen Liang , Lu Sheng
Published: June 2021

Background: Acute appendicitis (AA) is a common cause of abdominal pain encountering unnecessary surgeries in emergency departments. The present meta-analysis aims to assess the accuracy of abdominal ultrasound in suspected acute appendicitis cases in terms of sensitivity, specificity, and post-test odds for positive and negative results.

Materials and Methods: An extensive and systematic search was conducted in Medline (via PubMed), Cinahl (via Ebsco), Scopus, and Web of Sciences from 2010 till the end of March 2021. Two authors analyzed studies for inclusion, collected results, and conducted analyses separately. Examination of the histopathological tissue collected during appendectomy served as a gold standard for determining the final diagnosis of appendicitis. The accuracy was determined by evaluating sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), diagnostic odds ratio.

Results: Out of 3,193 references, a total of 18 studies were selected. Overall sensitivity of 77.2% (95% CI – 75.4–78.9%) and specificity of 60% (95% CI – 58–62%) were observed. The diagnostic odds ratio of 6.88(95% CI 1.99–23.82) was obtained.

Conclusion: Abdominal ultrasound shows significant accuracy of diagnosis in patients with suspected acute appendicitis.
Digital Extension Interactive Voice Response (IVR) mLearning: Lessons Learnt From Uganda Pig Value Chain
Authors: Dione Michel , Kangethe Edwin , Poole Elizabeth Jane , Ndiwa Nicholas , Ouma Emily , Dror Iddo
Published: June 2021

We assessed the effectiveness of Interactive Voice Response (IVR) technology in delivering biosecurity messages for the control of African swine fever (ASF) in Uganda using a randomized controlled trial (RCT) with 408 smallholder pig farmers. Our results show that IVR technology significantly improved knowledge of farmers who had not been exposed to training on biosecurity. Furthermore, it enhanced knowledge for farmers who had received face-to-face (f2f) training in biosecurity. This group of farmers recorded the highest knowledge gain following IVR training compared to farmers who did not receive f2f training. IVR technology was perceived by farmers as a new technology capable of transforming their lives because it is time efficient, has high potential for resource saving and flexibility. IVR also seems to be gender sensitive as it addresses some of the constraints women face in accessing conventional extension services such as time. IVR is an innovative way for delivery of advisory information to pig farmers. The scalability of IVR technology could further be explored and its feasibility assessed for wider use by the extension systems in Uganda and elsewhere.
Influence of Feeding Omega-3 Polyunsaturated Fatty Acids to Broiler Breeders on Indices of Immunocompetence, Gastrointestinal, and Skeletal Development in Broiler Chickens
Authors: Thanabalan Aizwarya , Kiarie Elijah G.
Published: June 2021

Modern broiler chickens are associated with rapid growth rates and superior feed efficiency. However, they are also susceptible to physiological and metabolic disorders (e.g., skin lesions, lameness, sudden death, enteric diseases, myopathies) that exert substantial economic losses to producers. This is further exacerbated by consumer pressure and mandated cessation of production practices such as indiscriminate use of antimicrobial growth promoters. Manipulation of broiler breeder (BB) nutrition and management can influence chick quality, robustness, and resilience to stressors in the production environment. The present review examines the role of feeding BB functional polyunsaturated omega-3 fatty acids (n-3 PUFA) and subsequent impact on the indices of immunocompetence, skeletal, and gastrointestinal (GIT) development in broiler chickens. Research in mammalian and avian models led evidence that perinatal feeding of long chain n-3 PUFA such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) engender transgenerational effects through regulation of a variety of biological processes including development of vital organs such as skeleton, brain and GIT. It is shown that feeding poultry breeders n-3 PUFA decreases inflammatory states and enriches hatching eggs with n-3 PUFA and immunoglobulins. Further evidence also shows that after 15 days of incubation, chicken embryos preferentially utilize long chain n-3 PUFA-critical for optimal cell, tissues, and organ development. Enrichment of n-3 PUFA in newly hatchling tissues reduce proinflammatory eicosanoids with consequences of enhanced bone mineralization. Dietary n-3 PUFA also modulates breeder GIT microbiota with consequences of microbial colonization and succession in chicks. As well, research shows that feeding poultry breeders n-3 PUFA bolsters progeny immunocompetence through enhanced passive immunity and antibody titres against routine vaccination. In conclusion, it appears that chicks may benefit from the incorporation of n-3 PUFA in the breeder diets; however, little attention is paid to fatty acids composition in breeder nutrition. We also highlight gaps in knowledge and future research perspectives.
UWB Based Relative Planar Localization with Enhanced Precision for Intelligent Vehicles
Authors: Wang Mingyang , Chen Xinbo , Lv Pengyuan , Jin Baobao , Wang Wei , Shen Yong
Published: June 2021

Along with the rapid development of advanced driving assistance systems for intelligent vehicles, essential functions such as forward collision warning and collaborative cruise control need to detect the relative positions of surrounding vehicles. This paper proposes a relative planar localization system based on the ultra-wideband (UWB) ranging technology. Three UWB modules are installed on the top of each vehicle. Because of the limited space on the vehicle roof compared with the ranging error, the traditional triangulation method leads to significant positioning errors. Therefore, an optimal localization algorithm combining homotopy and the Levenberg–Marquardt method is first proposed to enhance the precision. The triangular side lengths and directed area are introduced as constraints. Secondly, a UWB sensor error self-correction method is presented to further improve the ranging accuracy. Finally, we carry out simulations and experiments to show that the presented algorithm in this paper significantly improves the relative position and orientation precision of both the pure UWB localization system and the fusion system integrated with dead reckoning.
Preliminary Study of Body Temperature Emissivity in Rabbits Selected for Litter Size Residual Variability
Authors: Agea Iván , García María de la Luz , Argente María-José
Published: June 2021

A divergent selection for litter size residual variability has been carried out in rabbits during 12 generations. Litter size residual variability was estimated as phenotypic variance of litter size within females after correcting for the year-season and the parity-lactation status effects. Stress causes an increase in core body temperature. Infrared thermography (IRT) has been shown to be a useful technique for identifying changes in body temperature emissivity. The aim of this work is to study the correlated response to selection for litter size residual variability in body temperature emissivity at natural mating. Natural mating can be considered a stressful stimulus for does. Temperature was measured in the eyeball by IRT before mating (basal temperature) and after 5 min, 30 min, and 60 min in does of the lines selected to decrease and to increase litter size residual variability (i.e., the Low and the High lines). Both lines showed similar basal temperature. Eyeball temperature was increased slightly in the Low line from basal state to 5 min after stressful stimulus (from 35.69 °C to 36.32 °C), and this increase remained up to 60 min after stress (36.55 °C). The High line showed a higher temperature than the Low line at 30 min (+0.96 °C, p = 0.99). At 60 min, temperature was similar between lines. The evolution of temperature was different between lines: the High line reached the peak of temperature later than the Low line (at 30 min vs. 5 min), and its peak was higher compared to the Low line (36.95 °C vs. 36.32 °C). In conclusion, the does selected for reducing litter size variability showed a lower increase in temperature after a stressful stimulus, therefore showing lower stress and consequently better welfare.
From Traditional Food to Functional Food? Evaluation of Malvaceae Species as Novel Food Crops
Authors: Basheer Loai , Ben-Simchon Eyal , Cohen Alisa , Shelef Oren
Published: June 2021

Diversification of local food production can streamline supply chains, and ultimately increase food security. Research often focuses on improving existing crops by selection and by agro-technology rather than searching for novel crops. Plants that are traditionally eaten are interesting candidates for adaptation to commercialised agriculture. In this research, two Malvaceae species were explored as potential food crops, as the literature suggests Malvaceae exhibits valuable nutritional merits. This work examined Malva nicaeensis and Lavatera cretica, referred to as “Khubeza” (or “Hubeza”) as a generic term. The plants were experimentally cultivated in two different locations, their leaves were collected, and nutritive values compared. Khubeza leaves exhibited similar or better nutritive value to that of spinach, used here as a reference product. Thus, we conclude that “Khubeza” has potential to enhance food security, expand economic implementation, and to overall diversify agriculture, making it more resilient in the face of projected changes.
Biomass Yield of Selected Herbaceous and Woody Industrial Crops across Marginal Agricultural Sites with Shallow Soil
Authors: Reinhardt Jana , Hilgert Pia , von Cossel Moritz
Published: June 2021

Agricultural land in Europe is affected by low rooting depth (LRD) on 27.9 Mha. This marginal agricultural land can potentially be used to grow industrial crops without directly threatening food security or biodiversity conservation. However, little is known about the yield performance of industrial crops at LRD conditions. This study therefore compiles and discusses the meaningful data available in scientific literature. Twelve relevant industrial crops were identified for Europe. Currently, robust information on good growth suitability for LRD conditions is available for only one industrial crop, namely reed canary grass (RCG). Because this information was taken from field trial results from a single site, it remains unclear what role other growing conditions such as soil quality and climate play on both the yield level and the biomass quality of RCG under LRD conditions. These uncertainties about the quantitative as well as qualitative performance of industrial crop cultivation on marginal agricultural land characterized by LRD represent a major agronomic knowledge gap. Here, more knowledge needs to be compiled through both expanded crop science activities and improved international information exchange to make more optimal use of the large LRD areas available for the transition to a bioeconomy.
Durum Wheat Yield and N Uptake as Affected by N Source, Timing, and Rate in Two Mediterranean Environments
Authors: Pampana Silvia , Mariotti Marco
Published: June 2021

In nitrate vulnerable zones (NVZs), site-specific techniques are needed to match N availability with durum wheat (Triticum turgidum subsp. durum Desf.) requirements. Enhanced-efficiency fertilizers can improve efficient N supply and reduce leaching, contributing to sustainable agriculture. Two-year field experiments were carried out at two Mediterranean nitrate vulnerable zones in Central Italy (Pisa and Arezzo) to study the effects of nitrogen sources, timings, and application rates. The trial compared: (i) three N sources for the first topdressing application (urea, methylene urea, and urea with the nitrification inhibitor DMPP); (ii) two stages for the first topdressing N application (1st tiller visible—BBCH21 and 1st node detectable—BBCH31); (iii) two N rates: one based on the crop N requirements (Optimal—NO), the other based on action programme prescriptions of the two NVZs (Action Programme—NAP). Grain yield and yield components were determined, together with N uptake. The results showed that: (i) grain and biomass production were reduced with NAP at both locations; (ii) urea performed better than slow-release fertilizers; (iii) the best application time depended on the N source and location: in Pisa, enhanced-efficiency fertilizers achieved higher yields when applied earliest, while for urea the opposite was true; in Arezzo different N fertilizers showed similar performances between the two application timings. Different behaviors of topdressing fertilizers at the two localities could be related to the diverse patterns of temperatures and rainfall. Thus, optimal fertilization strategies would seem to vary according to environmental conditions.