bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Extreme Dust Storm in Agricultural Areas (Poland, the Greater Lowland)
Authors: Hojan Marcin , Rurek Mirosław , Więcław Mirosław , Krupa Adam
Published: February 2019

The article aims to present extreme aeolian processes observed in April 2011 in the fields of the Szamocin municipality in Pojezierze Wielkopolskie (the Great Poland Lakeland). Aeolian erosion of cropland was fostered by strong wind with gusts exceeding 15 m·s−1. The erosion was related to the movement of a deep barometric low from the Norwegian Sea over western Russia (Joachim barometric low). Geomorphological mapping of accumulated sandy aeolian covers was conducted. Morphometric measurements of aeolian covers were made considering their spatial distribution and the thickness of sediments that build them. Samples were collected from sediments to analyze grain size and the volume of deposited sediments was calculated. According to grain size analysis the accumulated fine-grained mineral material was well, moderately well and moderately sorted. The thickness of aeolian covers exceeded the thickness of covers presented in Polish literature so far. Calculations of the total volume of mineral sediment derived from small areas covered with aeolian erosion (a few hectares each) indicate significantly greater volumes than those provided in publications from other research areas in Poland. The described dust storms did not cause much damage in Poland. At the same time (8 April) in northern Germany, a dust storm led to a severe collision on the A19 highway. Eight people died in car accidents and dozens suffered serious injuries.
Traditional Diet and Environmental Contaminants in Coastal Chukotka II: Legacy POPs
Authors: Dudarev Alexey A. , Chupakhin Valery S. , Vlasov Sergey V. , Yamin-Pasternak Sveta
Published: February 2019

The article is the second in the series of four that present the results of a study on environmental contaminants in coastal Chukotka, conducted in the context of a multi-disciplinary investigation of indigenous foodways in the region. The article presents the results of the analysis of legacy Persistent Organic Pollutants (POPs) found in the samples of locally harvested food and indoor matters, collected in 2016 in coastal Chukotka. Temporal trends and circumpolar comparisons of POPs in food have been carried out. Estimated daily intakes (EDIs) of POPs by local food consumption were calculated based on the food intake frequencies (questionnaire data). Concentrations of the studied legacy POPs in marine mammal blubber were relatively high (up to 100–200 µg/kg ww) but not exceeding the allowable limits. Gray whale blubber and whale mantak were the most contaminated foods, followed by the ringed, spotted and bearded seal blubber, then by walrus blubber and fermented walrus (deboned walrus parts aged in subterranean pits, typically over a period of 6 months). At the backdrop of general decrease or invariability (compared to the previous coastal Chukotka study 15 years ago) of the majority of POPs, an increasing tendency of HCB, mainly in marine mammals, were noted. Legacy POPs in marine mammals sampled in Chukotka were generally much lower than in those sampled in Alaska and northern Canada. We suggest that the Alaska Coastal Current from the Bering Sea plays a major role in this phenomenon. Analyses of the additional sources of in-home food contamination (home-brewed alcohol, domestic insecticides) have revealed relatively high levels of HCHs, DDTs and PCBs, which still represent a share of dietary exposure of local people to POPs.
Can an Exercise-Based Educational and Motivational Intervention be Durably Effective in Changing Compliance to Physical Activity and Anthropometric Risk in People with Type 2 Diabetes? A Follow-Up Study
Authors: Gallè Francesca , Krakauer Jesse C. , Krakauer Nir Y. , Valerio Giuliana , Liguori Giorgio
Published: February 2019

Aims. A nine-month motivational exercise-based intervention was previously offered to subjects with type 2 diabetes (T2D). A year after the end of the intervention, compliance to physical activity (PA) and anthropometric indices of participants were analyzed to evaluate the durability of its effects. Methods. PA levels, expressed as total energy expenditure per week, were assessed with the International Physical Activity Questionnaire (IPAQ). Changes in Body Mass Index (BMI), A Body Shape Index (ABSI), Hip Index (HI) z-scores, the relative mortality risk related to each of these measures, and a combined Anthropometric Risk Index (ARI) were also evaluated. Results. Of a total of the 52 subjects examined (67.9% males, mean age 61.8 ± 6.0), 46 (88.4%) were still sufficiently active as defined by IPAQ thresholds at follow-up. PA levels, anthropometric indices and related risks improved at follow-up in respect to the baseline and to the end of the intervention, although only PA levels, BMI and related measures, and ARI risk changed significantly. Habitual PA increased significantly after the intervention (p < 0.01) and this increase correlated with changes in BMI z-scores (r = −0.29, p = 0.04). BMI risk was significantly lower (p < 0.01) in participants still active at follow-up. Conclusions. This study testifies to the persistence of compliance to PA and health benefits of a combined exercise-based and motivational intervention in subjects with T2D.
Assessment of Water Footprints of Consumption and Production in Transboundary River Basins at Country-Basin Mesh-Based Spatial Resolution
Authors: Wu Xia , Degefu Dagmawi Mulugeta , Yuan Liang , Liao Zaiyi , He Weijun , An Min , Zhang Zhaofang
Published: February 2019

Water is unevenly distributed globally. This uneven distribution is the reason behind the differences among geographical areas in terms of their water footprint of consumption and production. This gives the global trade of goods a unique feature. This characteristic of the water footprint might be used to address water scarcity and conflicts because water availability also has the same trend. Transboundary river basins are freshwater resources with a high probability of water scarcity and conflict because the water is claimed by multiple sovereign countries. In order to design sharing mechanisms for transboundary river basins that incorporate virtual water concept, it is key to identify the virtual water balance of country-basin units. A study addressing this research gap is not yet available. This article identified and discussed net virtual water importer and exporter sub-basins of transboundary rivers at a country-basin mesh based spatial resolution. The results of our study show that out of the 565 country-basin units surveyed in this article 391, 369, and 461 are net gray, green, and blue virtual water importers respectively. These sub-basins covers 58.37%, 47.52% and 57.52% of the total area covered by transboundary river basins and includes 0.65, 1.9, and around 2 billion people, respectively. The results depict that not only the water endowment of sub-basins is a determining factor for their water footprint of consumption and production, but also their social, economic, and demographic profiles. Furthermore, the water footprint of consumption and production within most of the country-basin units have a global feature. Hence, sustainable water management schemes within border-crossing basins should take into account not only the local but also the global water footprints of consumption and production. This can offer more options for sharing transboundary river basins water capital, thereby minimizing the probability of water scarcity and water conflicts.
Extensive Reliability Evaluation of Docking-Based Target-Fishing Strategies
Authors: Lapillo Margherita , Tuccinardi Tiziano , Martinelli Adriano , Macchia Marco , Giordano Antonio , Poli Giulio
Published: February 2019

The development of target-fishing approaches, aimed at identifying the possible protein targets of a small molecule, represents a hot topic in medicinal chemistry. A successful target-fishing approach would allow for the elucidation of the mechanism of action of all therapeutically interesting compounds for which the actual target is still unknown. Moreover, target-fishing would be essential for preventing adverse effects of drug candidates, by predicting their potential off-targets, and it would speed up drug repurposing campaigns. However, due to the huge number of possible protein targets that a small-molecule might interact with, experimental target-fishing approaches are out of reach. In silico target-fishing represents a valuable alternative, and examples of receptor-based approaches, exploiting the large number of crystallographic protein structures determined to date, have been reported in the literature. To the best of our knowledge, no proper evaluation of such approaches is, however, reported yet. In the present work, we extensively assessed the reliability of docking-based target-fishing strategies. For this purpose, a set of X-ray structures belonging to different targets was selected, and a dataset of compounds, including 10 experimentally active ligands for each target, was created. A target-fishing benchmark database was then obtained, and used to assess the performance of 13 different docking procedures, in identifying the correct target of the dataset ligands. Moreover, a consensus docking-based target-fishing strategy was developed and evaluated. The analysis highlighted that specific features of the target proteins could affect the reliability of the protocol, which however, proved to represent a valuable tool in the proper applicability domain. Our study represents the first extensive performance assessment of docking-based target-fishing approaches, paving the way for the development of novel efficient receptor-based target fishing strategies.
Age and Sex-Dependent Differences in the Neurochemical Characterization of Calcitonin Gene-Related Peptide-Like Immunoreactive (CGRP-LI) Nervous Structures in the Porcine Descending Colon
Authors: Makowska Krystyna , Gonkowski Slawomir
Published: February 2019

Neurons of the enteric nervous system (ENS) may undergo changes during maturation and aging, but knowledge of physiological stimuli-dependent changes in the ENS is still fragmentary. On the other hand, the frequency of many ENS-related intestinal illnesses depends on age and/or sex. The double immunofluorescence technique was used to study the influence of both of these factors on calcitonin gene-related peptide (CGRP)—positive enteric nervous structures—in the descending colon in young and adult female and castrated male pigs. The influence of age and gender on the number and neurochemical characterization (i.e., co-localization of CGRP with substance P, nitric oxide synthase, galanin, cocaine- and amphetamine-regulated transcript peptide and vesicular acetylcholine transporter) of CGRP-positive nerve structures in the colonic wall has been shown. These observations strongly suggest the participation of CGRP in adaptive processes in the ENS during GI tract maturation. Moreover, although the castration of males may mask some aspects of sex-dependent influences on the ENS, the sex-specific differences in CGRP-positive nervous structures were mainly visible in adult animals. This may suggest that the distribution and exact role of this substance in the ENS depend on the sex hormones.
Roles of SETD2 in Leukemia—Transcription, DNA-Damage, and Beyond
Authors: Skucha Anna , Ebner Jessica , Grebien Florian
Published: February 2019

The non-redundant histone methyltransferase SETD2 (SET domain containing 2; KMT3A) is responsible for tri-methylation of lysine 36 on histone H3 (H3K36me3). Presence of the H3K36me3 histone mark across the genome has been correlated with transcriptional activation and elongation, but also with the regulation of DNA mismatch repair, homologous recombination and alternative splicing. The role of SETD2 and the H3K36me3 histone mark in cancer is controversial. SETD2 is lost or mutated in various cancers, supporting a tumor suppressive role of the protein. Alterations in the SETD2 gene are also present in leukemia patients, where they are associated with aggressive disease and relapse. In line, heterozygous SETD2 loss caused chemotherapy resistance in leukemia cell lines and mouse models. In contrast, other studies indicate that SETD2 is critically required for the proliferation of leukemia cells. Thus, although studies of SETD2-dependent processes in cancer have contributed to a better understanding of the SETD2–H3K36me3 axis, many open questions remain regarding its specific role in leukemia. Here, we review the current literature about critical functions of SETD2 in the context of hematopoietic malignancies.
Development of the VIGS System in the Dioecious Plant Silene latifolia
Authors: Fujita Naoko , Kazama Yusuke , Yamagishi Noriko , Watanabe Kyoko , Ando Saki , Tsuji Hiroyuki , Kawano Shigeyuki , Yoshikawa Nobuyuki , Komatsu Ken
Published: February 2019

(1) Background: Silene latifolia is a dioecious plant, whose sex is determined by XY-type sex chromosomes. Microbotryum lychnidis-dioicae is a smut fungus that infects S. latifolia plants and causes masculinization in female flowers, as if Microbotryum were acting as a sex-determining gene. Recent large-scale sequencing efforts have promised to provide candidate genes that are involved in the sex determination machinery in plants. These candidate genes are to be analyzed for functional characterization. A virus vector can be a tool for functional gene analyses; (2) Methods: To develop a viral vector system in S. latifolia plants, we selected Apple latent spherical virus (ALSV) as an appropriate virus vector that has a wide host range; (3) Results: Following the optimization of the ALSV inoculation method, S. latifolia plants were infected with ALSV at high rates in the upper leaves. In situ hybridization analysis revealed that ALSV can migrate into the flower meristems in S. latifolia plants. Successful VIGS (virus-induced gene silencing) in S. latifolia plants was demonstrated with knockdown of the phytoene desaturase gene. Finally, the developed method was applied to floral organ genes to evaluate its usability in flowers; (4) Conclusion: The developed system enables functional gene analyses in S. latifolia plants, which can unveil gene functions and networks of S. latifolia plants, such as the mechanisms of sex determination and fungal-induced masculinization.
Comprehensive Comparative Analysis of Cholesterol Catabolic Genes/Proteins in Mycobacterial Species
Authors: van Wyk Rochelle , van Wyk Mari , Mashele Samson Sitheni , Nelson David R. , Syed Khajamohiddin
Published: February 2019

In dealing with Mycobacterium tuberculosis, the causative agent of the deadliest human disease—tuberculosis (TB)—utilization of cholesterol as a carbon source indicates the possibility of using cholesterol catabolic genes/proteins as novel drug targets. However, studies on cholesterol catabolism in mycobacterial species are scarce, and the number of mycobacterial species utilizing cholesterol as a carbon source is unknown. The availability of a large number of mycobacterial species’ genomic data affords an opportunity to explore and predict mycobacterial species’ ability to utilize cholesterol employing in silico methods. In this study, comprehensive comparative analysis of cholesterol catabolic genes/proteins in 93 mycobacterial species was achieved by deducing a comprehensive cholesterol catabolic pathway, developing a software tool for extracting homologous protein data and using protein structure and functional data. Based on the presence of cholesterol catabolic homologous proteins proven or predicted to be either essential or specifically required for the growth of M. tuberculosis H37Rv on cholesterol, we predict that among 93 mycobacterial species, 51 species will be able to utilize cholesterol as a carbon source. This study’s predictions need further experimental validation and the results should be taken as a source of information on cholesterol catabolism and genes/proteins involved in this process among mycobacterial species.
A Review of Curcumin and Its Derivatives as Anticancer Agents
Authors: Tomeh Mhd Anas , Hadianamrei Roja , Zhao Xiubo
Published: February 2019

Cancer is the second leading cause of death in the world and one of the major public health problems. Despite the great advances in cancer therapy, the incidence and mortality rates of cancer remain high. Therefore, the quest for more efficient and less toxic cancer treatment strategies is still at the forefront of current research. Curcumin, the active ingredient of the Curcuma longa plant, has received great attention over the past two decades as an antioxidant, anti-inflammatory, and anticancer agent. In this review, a summary of the medicinal chemistry and pharmacology of curcumin and its derivatives in regard to anticancer activity, their main mechanisms of action, and cellular targets has been provided based on the literature data from the experimental and clinical evaluation of curcumin in cancer cell lines, animal models, and human subjects. In addition, the recent advances in the drug delivery systems for curcumin delivery to cancer cells have been highlighted.
Chloride Channels in Astrocytes: Structure, Roles in Brain Homeostasis and Implications in Disease
Authors: Elorza-Vidal Xabier , Gaitán-Peñas Héctor , Estévez Raúl
Published: February 2019

Astrocytes are the most abundant cell type in the CNS (central nervous system). They exert multiple functions during development and in the adult CNS that are essential for brain homeostasis. Both cation and anion channel activities have been identified in astrocytes and it is believed that they play key roles in astrocyte function. Whereas the proteins and the physiological roles assigned to cation channels are becoming very clear, the study of astrocytic chloride channels is in its early stages. In recent years, we have moved from the identification of chloride channel activities present in astrocyte primary culture to the identification of the proteins involved in these activities, the determination of their 3D structure and attempts to gain insights about their physiological role. Here, we review the recent findings related to the main chloride channels identified in astrocytes: the voltage-dependent ClC-2, the calcium-activated bestrophin, the volume-activated VRAC (volume-regulated anion channel) and the stress-activated Maxi-Cl−. We discuss key aspects of channel biophysics and structure with a focus on their role in glial physiology and human disease.
Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-α Ratio in Tears of Patients with Perennial Allergic Conjunctivitis: A Preliminary Study
Authors: Salazar Alberto , Casanova-Méndez Israel , Pacheco-Quito Michele , Velázquez-Soto Henry , Ayala-Balboa Julio , Graue-Hernández Enrique O. , Serafín-López Jeanet , Jiménez-Martínez María C.
Published: February 2019

Allergic conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in perennial allergic conjunctivitis (PAC), and interleukins (IL)-1β, IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-α, were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19+IL-10+ cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19+CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-α in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with allergic conjunctivitis, characterized by the low expression of IL-10 in circulating CD19+CD38+ Bregs subsets and an inverted tear IL-10/TNF-α ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels.
Size-Dependent Effect of Silver Nanoparticles on the Tumor Necrosis Factor α-Induced DNA Damage Response
Authors: Fehaid Alaa , Taniguchi Akiyoshi
Published: February 2019

Silver nanoparticles (AgNPs) are widely used in many consumer products due to their anti-inflammatory properties. Therefore, the effect of exposure to AgNPs should be investigated in diseased states in addition to healthy ones. Tumor necrosis factor-α (TNFα) is a major cytokine that is highly expressed in many diseased conditions, such as inflammatory diseases, sepsis, and cancer. We investigated the effects of two different sizes of AgNPs on the TNFα-induced DNA damage response. Cells were exposed to 10 and 200 nm AgNPs separately and the results showed that the 200 nm AgNPs had a lower cytotoxic effect with a higher percent of cellular uptake compared to the 10 nm AgNPs. Moreover, analysis of reactive oxygen species (ROS) generation and DNA damage indicated that TNFα-induced ROS-mediated DNA damage was reduced by 200 nm AgNPs, but not by 10 nm AgNPs. Tumor necrosis factor receptor 1 (TNFR1) was localized on the cell surface after TNFα exposure with or without 10 nm AgNPs. In contrast, the expression of TNFR1 on the cell surface was reduced by the 200 nm AgNPs. These results suggested that exposure of cells to 200 nm AgNPs reduces the TNFα-induced DNA damage response via reducing the surface expression of TNFR1, thus reducing the signal transduction of TNFα.
Identifying Genome-Wide Sequence Variations and Candidate Genes Implicated in Self-Incompatibility by Resequencing Fragaria viridis
Authors: Du Jianke , Lv Yan , Xiong Jinsong , Ge Chunfeng , Iqbal Shahid , Qiao Yushan
Published: February 2019

It is clear that the incompatibility system in Fragaria is gametophytic, however, the genetic mechanism behind this remains elusive. Eleven second-generation lines of Fragaria viridis with different compatibility were obtained by manual self-pollination, which can be displayed directly by the level of fruit-set rate. We sequenced two second-generation selfing lines with large differences in fruit-set rate: Ls-S2-53 as a self-incompatible sequencing sample, and Ls-S2-76 as a strong self-compatible sequencing sample. Fragaria vesca was used as a completely self-compatible reference sample, and the genome-wide variations were identified and subsequently annotated. The distribution of polymorphisms is similar on each chromosome between the two sequencing samples, however, the distribution regions and the number of homozygous variations are inconsistent. Expression pattern analysis showed that six candidate genes were significantly associated with self-incompatibility. Using F. vesca as a reference, we focused our attention on the gene FIP2-like (FH protein interacting protein), associated with actin cytoskeleton formation, as the resulting proteins in Ls-S2-53 and Ls-S2-76 have each lost a number of different amino acids. Suppression of FIP2-like to some extent inhibits germination of pollen grains and growth of pollen tubes by reducing F-actin of the pollen tube tips. Our results suggest that the differential distribution of homozygous variations affects F. viridis fruit-set rate and that the fully encoded FIP2-like can function normally to promote F-actin formation, while the new FIP2-like proteins with shortened amino acid sequences have influenced the (in)compatibility of two selfing lines of F. viridis.
Loads and Response of a Tension Leg Platform Wind Turbine with Non-Rotating Blades: An Experimental Study
Authors: Murfet Timothy , Abdussamie Nagi
Published: February 2019

This paper describes model testing of a Tension Leg Platform Wind Turbine (TLPWT) with non-rotating blades to better understand its motion and tendon responses when subjected to combined wind and unidirectional regular wave conditions. The TLPWT structure is closely based on the National Renewable Energy Laboratory (NREL) 5 MW concept. Multiple free decay tests were performed to evaluate the natural periods of the model in the key degrees of freedom, whilst Response Amplitude Operators (RAOs) were derived to show the motion and tendon characteristics. The natural periods in surge and pitch motions evaluated from the decay tests had a relatively close agreement to the theoretical values. Overall, the tested TLPWT model exhibited typical motion responses to that of a generalised TLP with significant surge offsets along with stiff heave and pitch motions. The maximum magnitudes for the RAOs of surge motion and all tendons occurred at the longest wave period of 1.23 s (~13.0 s at full-scale) tested in this study. From the attained results, there was evidence that static wind loading on the turbine structure had some impact on the motions and tendon response, particularly in the heave direction, with an average increase of 13.1% in motion amplitude for the tested wind conditions. The wind had a negligible effect on the surge motion and slightly decreased the tendon tensions in all tendons. The results also showed the set-down magnitudes amounting to approximately 2–5% of the offset. Furthermore, the waves are the dominant factor contributing to the set-down of the TLPWT, with a minimal contribution from the static wind loading. The results of this study could be used for calibrating numerical tools such as CFD codes.
Combining Blockchain Technology and the Physical Internet to Achieve Triple Bottom Line Sustainability: A Comprehensive Research Agenda for Modern Logistics and Supply Chain Management
Authors: Treiblmaier Horst
Published: February 2019

Integrating triple bottom line (TBL) goals into supply chains (SCs) is a challenging task which necessitates the careful coordination of numerous stakeholders’ individual interests. Recent technological advancements can impact TBL sustainability by changing the design, structure, and management of modern SCs. Blockchain technology enables immutable data records and facilitates a shared data view along the supply chain. The Physical Internet (PI) is an overarching framework that can be applied to create a layered and comprehensive view of the SC. In this conceptual paper, I define and combine these technologies and derive several high-level research areas and research questions (RQ) to investigate adoption and management as well as structural SC issues. I suggest a theory-based research agenda for the years to come that exploits the strengths of rigorous academic research, while remaining relevant for industry. Furthermore, I suggest various well-established theories to tackle the respective research questions and provide specific directions for future research.
Development and Testing of Advanced Cork Composite Sandwiches for Energy-Absorbing Structures
Authors: Kaczyński Paweł , Ptak Mariusz , A. O. Fernandes Fábio , Chybowski Leszek , Wilhelm Johannes , J. Alves de Sousa Ricardo
Published: February 2019

Cork is a sustainable material with remarkable properties. In addition to its main application as wine stoppers, it has also been employed as a sound and thermal insulator in facades, building roofs, aeronautical applications, and, more recently, in impact energy absorption systems. In its natural form, cork is mainly used in wine stopper manufacturing, but for other applications, cork compounds are usually employed, which makes it possible to manufacture complex geometries with nearly isotropic behavior. In this work, an attempt was made to merge the desirable properties of two different cork materials (agglomerated and expanded black) into cork composite sandwich structures. These structures were tested according to impact conditions typically experienced by energy-absorbing liners used in personal safety devices. Additionally, the performance dependency on the working temperature was analyzed. The sole black, expanded cork (EC159) and agglomerated cork (AC199A and AC216) were tested in 500 J impacts. It was found that black cork was characterized by superior thermal stability, while expanded cork allowed absorbing high energies. In the second stage, the composites consisting of both tested materials were tested in 100 J impact scenarios. The combination of two materials of different properties enabled reduction of the peak force exerted on a helmet user’s head during the impact by about 10% compared to agglomerated specimens. Additionally, it was proved that there was no influence of the glue used to join different cork types.
A Review of Supercapacitors Based on Graphene and Redox-Active Organic Materials
Authors: Li Qi , Horn Michael , Wang Yinong , MacLeod Jennifer , Motta Nunzio , Liu Jinzhang
Published: February 2019

Supercapacitors are a highly promising class of energy storage devices due to their high power density and long life cycle. Conducting polymers (CPs) and organic molecules are potential candidates for improving supercapacitor electrodes due to their low cost, large specific pseudocapacitance and facile synthesis methods. Graphene, with its unique two-dimensional structure, shows high electrical conductivity, large specific surface area and outstanding mechanical properties, which makes it an excellent material for lithium ion batteries, fuel cells and supercapacitors. The combination of CPs and graphene as electrode material is expected to boost the properties of supercapacitors. In this review, we summarize recent reports on three different CP/graphene composites as electrode materials for supercapacitors, discussing synthesis and electrochemical performance. Novel flexible and wearable devices based on CP/graphene composites are introduced and discussed, with an eye to recent developments and challenges for future research directions.
A Novel Hybrid Algorithm for Minimum Total Dominating Set Problem
Authors: Yuan Fuyu , Li Chenxi , Gao Xin , Yin Minghao , Wang Yiyuan
Published: February 2019

The minimum total dominating set (MTDS) problem is a variant of the classical dominating set problem. In this paper, we propose a hybrid evolutionary algorithm, which combines local search and genetic algorithm to solve MTDS. Firstly, a novel scoring heuristic is implemented to increase the searching effectiveness and thus get better solutions. Specially, a population including several initial solutions is created first to make the algorithm search more regions and then the local search phase further improves the initial solutions by swapping vertices effectively. Secondly, the repair-based crossover operation creates new solutions to make the algorithm search more feasible regions. Experiments on the classical benchmark DIMACS are carried out to test the performance of the proposed algorithm, and the experimental results show that our algorithm performs much better than its competitor on all instances.
λ-Carrageenan Oligosaccharides of Distinct Anti-Heparanase and Anticoagulant Activities Inhibit MDA-MB-231 Breast Cancer Cell Migration
Authors: Groult Hugo , Cousin Rémi , Chot-Plassot Caroline , Maura Maheva , Bridiau Nicolas , Piot Jean-Marie , Maugard Thierry , Fruitier-Arnaudin Ingrid
Published: February 2019

In tumor development, the degradation of heparan sulfate (HS) by heparanase (HPSE) is associated with cell-surface and extracellular matrix remodeling as well as the release of HS-bound signaling molecules, allowing cancer cell migration, invasion and angiogenesis. Because of their structural similarity with HS, sulfated polysaccharides are considered a promising source of molecules to control these activities. In this study, we used a depolymerisation method for producing λ-carrageenan oligosaccharides (λ-CO), with progressive desulfation over time. These were then used to investigate the influence of polymeric chain length and degree of sulfation (DS) on their anti-HPSE activity. The effects of these two features on λ-CO anticoagulant properties were also investigated to eliminate a potential limitation on the use of a candidate λ-CO as a chemotherapeutic agent. HPSE inhibition was mainly related to the DS of λ-CO, however this correlation was not complete. On the other hand, both chain length and DS modulated λ-CO activity for factor Xa and thrombin IIa inhibition, two enzymes that are involved in the coagulation cascade, and different mechanisms of inhibition were observed. A λ-carrageenan oligosaccharide of 5.9 KDa was identified as a suitable anticancer candidate because it displayed one of the lowest anticoagulant properties among the λ-CO produced, while showing a remarkable inhibitory effect on MDA-MB-231 breast cancer cell migration.
Programmable Spectral Filter in C-Band Based on Digital Micromirror Device
Authors: Gao Yunshu , Chen Xiao , Chen Genxiang , Tan Zhongwei , Chen Qiao , Dai Dezheng , Zhang Qian , Yu Chao
Published: February 2019

Optical filters have been adopted in many applications such as reconfigurable telecommunication switches, tunable lasers and spectral imaging. However, most of commercialized filters based on a micro-electrical-mechanical system (MEMS) only provide a minimum bandwidth of 25 GHz in telecom so far. In this work, the programmable filter based on a digital micromirror device (DMD) experimentally demonstrated a minimum bandwidth of 12.5 GHz in C-band that matched the grid width of the International Telecommunication Union (ITU) G.694.1 standard. It was capable of filtering multiple wavebands simultaneously and flexibly by remotely uploading binary holograms onto the DMD. The number of channels and the center wavelength could be adjusted independently, as well as the channel bandwidth and the output power. The center wavelength tuning resolution of this filter achieved 0.033 nm and the insertion loss was about 10 dB across the entire C-band. Since the DMD had a high power handling capability (25 KW/cm2) of around 200 times that of the liquid crystal on silicon (LCoS) chip, the DMD-based filters are expected to be applied in high power situations.
Connector-Free World-to-Chip Interconnection for Microfluidic Devices
Authors: Song In-Hyouk , Park Taehyun
Published: February 2019

In the development of functional lab-on-a-chip (LOC), there is a need to produce a reliable and high pressure connection between capillary tubes and microfluidic devices for carrying fluids. The current technologies still have limitations in achieving ideal interconnection since they are bulky, expensive or complicated. In this paper, a novel connector-free technique using an interference fit mechanism is introduced for world-to-chip interconnection. The proposed technique has considerable potential for replacing current interconnection tools for microfluidic devices due to the advantages including no chemical contamination, easy plugging, enough strength to sustain pressure, high density integration, simple and rapid integration.
Flavor Profile Evolution of Bottle Aged Rosé and White Wines Sealed with Different Closures
Authors: Ling Meng-Qi , Xie Han , Hua Yu-Bo , Cai Jian , Li Si-Yu , Lan Yi-Bin , Li Ruo-Nan , Duan Chang-Qing , Shi Ying
Published: February 2019

Bottle aging is the final stage before wines are drunk, and is considered as a maturation time when many chemical changes occur. To get a better understanding of the evolution of wines’ flavor profile, the flavor compounds (phenolic and volatile compounds), dissolved oxygen (DO), and flavor characters (OAVs and chromatic parameters) of rosé and dry white wines bottled with different closures were determined after 18 months’ bottle aging. The results showed the main phenolic change trends of rosé wines were decreasing while the trends of white wines were increasing, which could be the reason for their unique DO changing behaviors. Volatile compounds could be clustered into fluctuating, increasing, and decreasing groups using k-means algorithm. Most volatile compounds, especially some long-chain aliphatic acid esters (octanoates and decanoates), exhibited a lower decrease rate in rosé wines sealed with natural corks and white wines with screw caps. After 18 months of bottle aging, wines treated with natural corks and their alternatives could be distinguished into two groups based on flavor compounds via PLS-DA. As for flavor characters, the total intensity of aroma declined obviously compared with their initial counterparts. Rosé wines exhibit visual difference in color, whereas such a phenomenon was not observed in white wines.
Multi-Steps Fragmentation-Ion Trap Mass Spectrometry Coupled to Liquid Chromatography Diode Array System for Investigation of Olaparib Related Substances
Authors: Khedr Alaa , El-Hay Soad S. Abd , Kammoun Ahmed K.
Published: February 2019

A high-performance liquid chromatography-diode array-mass spectrometric (LC-DAD-MS) method was developed and validated to investigate the related substances of olaparib (OLA) in bulk form. OLA was exposed to acid–base hydrolysis, boiling, oxidation with hydrogen peroxide, and UV light followed by LC-DAD-MS analysis. OLA and OLA-related substances were simultaneously and quantitatively monitored by DAD at 278 nm and triple quadrupole mass spectrometry (QQQ-MS). The investigated compounds were auto-scanned by an ion trap MS which applied positive and negative modes separately. The fragmentation pathway was confirmed by applying multi-steps fragmentation to identify the resulted cleaved ions and their parent ion. OLA was found to be sensitive to the alkaline hydrolysis and less sensitive to UV light. Two major hydrolytic degradation products, including the protonated molar ions m/z 299 and m/z 367, were identified. Three potential impurities were also characterized. The LC-MS limit of detection (LOD) and limit of quantification (LOQ) were 0.01 and 0.05 ng/µL, respectively. The quantitative results obtained by LC-DAD was comparable with that of LC-QQQ-MS. The proposed method shows good intra-day and inter-day precision with relative standard deviation (RSD) <2%.
Antioxidant Activities of Vaccinium vitis-idaea L. Leaves within Cultivars and Their Phenolic Compounds
Authors: Raudone Lina , Vilkickyte Gabriele , Pitkauskaite Lina , Raudonis Raimondas , Vainoriene Rimanta , Motiekaityte Vida
Published: February 2019

Lingonberry leaves are the subject of numerous studies because of antioxidant properties, positive influence on the health and potential use in the prevention and treatment of chronic diseases. In this work, the radical scavenging, reducing, chelating activities, and phenolic composition of ten lingonberry leaves cultivars, one subspecies, and one variety were investigated. Furthermore, the antioxidant activity of individual phenolic compounds, that can be found in lingonberry leaves, were analyzed, and structure-activity relationship was determined. Wide diversity for phenolic profile and antioxidant properties of lingonberry leaves has been observed in the present material. Cultivars ‘Kostromskaja rozovaja’, ‘Rubin’, and Vaccinium vitis-idaea var. leucocarpum surpassed all others tested cultivars and lower taxa by contents of phenolic compounds and antioxidant activity. Leaves of lingonberry cultivars and lower taxa are rich in arbutin, flavonol glycosides, proanthocyanidins, and the latter were considered to be the major contributor to antioxidant properties of lingonberry leaves.
Self-Assembled Vanadium Oxide Nanoflakes for p-Type Ammonia Sensors at Room Temperature
Authors: Yin Haihong , Song Changqing , Wang Zhiliang , Shao Haibao , Li Yi , Deng Honghai , Ma Qinglan , Yu Ke
Published: February 2019

VO2(B), VO2(M), and V2O5 are the most famous compounds in the vanadium oxide family. Here, their gas-sensing properties were investigated and compared. VO2(B) nanoflakes were first self-assembled via a hydrothermal method, and then VO2(M) and V2O5 nanoflakes were obtained after a heat-phase transformation in nitrogen and air, respectively. Their microstructures were evaluated using X-ray diffraction and scanning and transmission electron microscopies, respectively. Gas sensing measurements indicated that VO2(M) nanoflakes were gas-insensitive, while both VO2(B) and V2O5 nanoflakes were highly selective to ammonia at room temperature. As ammonia sensors, both VO2(B) and V2O5 nanoflakes showed abnormal p-type sensing characteristics, although vanadium oxides are generally considered as n-type semiconductors. Moreover, V2O5 nanoflakes exhibited superior ammonia sensing performance compared to VO2(B) nanoflakes, with one order of magnitude higher sensitivity, a shorter response time of 14–22 s, and a shorter recovery time of 14–20 s. These characteristics showed the excellent potential of V2O5 nanostructures as ammonia sensors.
Maternal Obesity During Pregnancy and Lactation Influences Offspring Obesogenic Adipogenesis but Not Developmental Adipogenesis in Mice
Authors: Sellayah Dyan , Thomas Hugh , Lanham Stuart A. , Cagampang Felino R.
Published: February 2019

Obesity is an escalating health crisis of pandemic proportions and by all accounts it has yet to reach its peak. Growing evidence suggests that obesity may have its origins in utero. Recent studies have shown that maternal obesity during pregnancy may promote adipogenesis in offspring. However, these studies were largely based on cell culture models. Whether or not maternal obesity impacts on offspring adipogenesis in vivo remains to be fully established. Furthermore, in vivo adipogenic differentiation has been shown to happen at distinct time periods, one during development (developmental adipogenesis—which is complete by 4 weeks of age in mice) and another in adulthood in response to feeding a high-fat (HF) diet (obesogenic adipogenesis). We therefore set out to determine whether maternal obesity impacted on offspring adipocyte hyperplasia in vivo and whether maternal obesity impacted on developmental or obesogenic adipogenesis, or both. Our findings reveal that maternal obesity is associated with enhanced obesogenic adipogenesis in HF-fed offspring. Interestingly, in newly weaned (4-week-old) offspring, maternal obesity is associated with adipocyte hypertrophy, but there were no changes in adipocyte number. Our results suggest that maternal obesity impacts on offspring obesogenic adipogenesis but does not affect developmental adipogenesis.
Dietary Components That May Influence the Disturbed Gut Microbiota in Chronic Kidney Disease
Authors: Mafra Denise , Borges Natália , Alvarenga Livia , Esgalhado Marta , Cardozo Ludmila , Lindholm Bengt , Stenvinkel Peter
Published: February 2019

Gut microbiota imbalance is common in patients with chronic kidney disease (CKD) and associates with factors such as increased circulating levels of gut-derived uremic toxins, inflammation, and oxidative stress, which are linked to cardiovascular disease and increased morbimortality. Different nutritional strategies have been proposed to modulate gut microbiota, and could potentially be used to reduce dysbiosis in CKD. Nutrients like proteins, fibers, probiotics, and synbiotics are important determinants of the composition of gut microbiota and specific bioactive compounds such as polyphenols present in nuts, berries. and fruits, and curcumin, may also play a key role in this regard. However, so far, there are few studies on dietary components influencing the gut microbiota in CKD, and it is therefore not possible to conclude which nutrients should be prioritized in the diet of patients with CKD. In this review, we discuss some nutrients, diet patterns and bioactive compounds that may be involved in the modulation of gut microbiota in CKD and provide the background and rationale for studies exploring whether nutritional interventions with these dietary components could be used to alleviate the gut dysbiosis in patients with CKD.
Subjects’ Perception in Quantifying Printed and Digital Photos of Food Portions
Authors: Nichelle Pryscila G. , Almeida Claudia C. B. , Camey Suzi A. , Garmus Lenine M. , Elias Vanessa C. M. , Marchioni Dirce M. , da Silva Danielle G. , Ocke Marga C. , Slimani Nadia , Fisberg Regina M. , Crispim Sandra P.
Published: February 2019

Although digital photos have the potential to improve the precision of reported portions in dietary assessment, there are few studies investigating its accuracy in comparison to printed photos. The aim of this study was to evaluate the perception of adults in quantifying food portion sizes using printed and digital photos, displayed on computer-screens and tablets. In total, 1165 evaluations were performed for 60 photos of portion sizes in Brazil. Each participant (n = 58) attended two sessions in the study center, with an interval of at least one week. In each session, twelve food portions were prepared and randomly evaluated by each participant in its printed and digital forms. The mean error (difference between the estimated and true portions) was not significantly different between the printed photos (2.1 g ± 47.2) and the digital ones (−6.4 g ± 53.7). The agreement on using the printed and digital photos was 91% and 90%, respectively. Furthermore, the use of the tablet was more prone to underestimation when compared to printed and computer-screen photos (p < 0.001). Overall, participants did not present major difficulties in perceiving the portion sizes using the printed and digital photos, but the use of tablets led to less accurate results, indicating that this needs to be further evaluated.
Resting Energy Expenditure, Body Composition and Phase Angle in Anorectic, Ballet Dancers and Constitutionally Lean Males
Authors: Marra Maurizio , Sammarco Rosa , De Filippo Emilia , De Caprio Carmela , Speranza Enza , Contaldo Franco , Pasanisi Fabrizio
Published: February 2019

Background: The prevalence of anorexia nervosa among males is increasing but few data are available in the literature. This cross sectional study aims to evaluate resting energy expenditure (REE) and phase angle as a marker of qualitative changes of fat free mass (FFM) in three leanness groups as compared with control subjects. Methods: 17 anorectic (AN) males, 15 constitutionally lean (CL) individuals, 12 ballet dancers (DC), and 18 control (CTR) subjects were evaluated. REE was measured by indirect calorimetry (V max29- Sensormedics), and body composition was evaluated by bioimpedance analysis (BIA) at 50 kHz (DS Medica). Phase angle (a bioimpedance variable related to nutritional status) was used to evaluate differences in FFM characteristics between these three types of leanness. Results: REE, adjusted for FFM and fat mass (FM), were significantly higher in CL and lower in AN individuals (1783 ± 47 vs. 1291 ± 58 kcal, p < 0.05) compared to the other groups. Body composition was similar in AN and CL whereas dancers had the highest FFM (58.9 ± 4.8 kg, p < 0.05); anorectic males showed the lowest phase angle (5.8 ± 1.2 degrees vs. other groups, p < 0.05) and dancers the highest phase angle (7.9 ± 0.7 degree vs. other group, p < 0.05). Conclusions: Our findings confirm that phase angle could be a useful marker of qualitative changes, above all in the field of sport activities. On the other hand, there is the need to further evaluate the relationship between resting energy expenditure, body composition and endocrine status in different conditions of physical activity and dietary intake.