bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapping the research of mitochondria and Parkinson’s disease: a bibliometric analysis
Authors: Chen Yan-Jun , Xie Ming-Rong , Zhou Sheng-Qiang , Liu Fang
Published: September 2024

Background

Parkinson’s disease (PD) is a chronic, progressive neurodegenerative disorder primarily affecting the elderly. Relevant studies suggest a significant connection between the mitochondria and PD. Publications exploring this connection have steadily increased in recent years. This study employs a bibliometric approach to comprehensively analyze the current status and future directions of the research on mitochondria and PD.

Method

We retrieved data from the Web of Science database and used CiteSpace, VOSviewer, and “Bibliometrix” software to visually analyze various aspects of the research field. These aspects included the number of published papers, contributing countries and institutions, authors, publishing journals, cited references, and keywords.

Results

Our analysis identified a total of 3,291 publications involving 14,670 authors from 2,836 organizations across 78 countries. The publication volume exhibited a continuous upward trend from 1999 to 2023. The United States emerged as the leading force in this research area, contributing the highest number of high-quality publications. Notably, the United States collaborated extensively with Germany and the United Kingdom. The University of Pittsburgh stood out as the most prolific institution. Harvard University had the highest academic influence and closely cooperated with the University of Pittsburgh, Juntendo University, and McGill University. Dr. Hattori Nobutaka was identified as the most prolific author, while Dr. Youle, Richard J emerged as the most influential author based on the highest average citation frequency. The Journal of Neurochemistry was the most published journal. The most co-cited paper was titled “Hereditary early-onset Parkinson’s disease caused by mutations in PINK1.” The major keywords included oxidative stress, alpha-synuclein, pink1, mitophagy, and mitochondrial dysfunction. Mitofusin 2, ubiquitin, and mitochondrial quality control have been identified as new research hotspots in recent years.

Conclusion

Mitochondria-PD research is experiencing a steady increase in activity, fueled by increasing close collaboration between countries and different institutions. However, there is a need to further strengthen collaboration and communication between developed and developing nations. Current research has focused on the specific mechanisms of mitochondrial dysfunction and their relationship with PD. Mitofusin 2, ubiquitin, and mitochondrial quality control are positioned to be the hotspots and future research directions.
Cognitive function in dyslipidemia patients: exploring the impact of statins
Authors: Wang Wenxiao , Li Xin
Published: September 2024

Background

Evidence regarding the relationship between the use of statins and cognitive outcomes presents varying findings. This study aims to analyze the relationship between sustained statin use and cognitive performance in dyslipidemia patients.

Methods

This study presents findings from the Beijing Ageing Brain Rejuvenation Initiative (BABRI) study, in which a cohort of community-dwelling dyslipidemia patients (Entire sample, N = 1,062, aged 50–86) was recruited. Participants were divided into two groups based on their sustained use statins (Statins group, N = 677) or not use any lipid-lowering agents (Untreated group, N = 385). Furthermore, the entire sample was stratified by age into the middle-aged sample (N = 451) and the older people sample (N = 611), following a similar categorization based on statin application. ANCOVA was used to evaluate the relationship between sustained statin use and cognitive function.

Results

Overall, in the total sample, the statins group demonstrated better cognition in overall cognition, memory, visuospatial ability, attention, executive function, and language domains compared to the untreated group. Moreover, the statins group only showed better performance in attention among the middle-aged sample. In the older people sample, statins group exhibited superior cognitive performance across various cognitive domains compared to untreated group.

Conclusion

Among dyslipidemia patients in Beijing community, sustained statin users exhibited superior cognitive function across all domains compared to untreated individuals, with particularly noticeable improvements among those aged 65 and above. These findings underscore the protective effect of statins on cognitive function in dyslipidemia patients, highlighting significant benefits for the older people population.
Inclusion, reporting and analysis of demographic variables in chronobiology and sleep research
Authors: Tir Selma , White Rhiannon , Spitschan Manuel
Published: September 2024

Many aspects of sleep and circadian physiology are sensitive to participant-level characteristics. While recent research robustly highlights the importance of considering participant-level demographic information, the extent to which this information is consistently collected, and reported in the literature, remains unclear. This article investigates study sample characteristics within the published sleep and chronobiology research over the past 40 years. 6,777 articles were identified and a random sample of 20% was included. The reporting of sample size, age, sex, gender, ethnicity, level of education, socio-economic status, and profession of the study population was scored, and any reported aggregate summary statistics for these variables were recorded. We observed a significant upward trend in the reporting and analysis of demographic variables in sleep and chronobiology research. However, we found that while > 90% of studies reported age or sex, all other variables were reported in < 25% of cases. Reporting quality was highly variable, indicating an opportunity to standardize reporting guidelines for participant-level characteristics to facilitate Meta analyses.
Doublecortin-immunoreactive neurons in the piriform cortex are sensitive to the long lasting effects of early life stress
Authors: Abellán-Álvaro María , Teruel-Sanchis Anna , Madeira Maria Francisca , Lanuza Enrique , Santos Mónica , Agustín-Pavón Carmen
Published: September 2024

The olfactory system is a niche of continuous structural plasticity, holding postnatal proliferative neurogenesis in the olfactory bulbs and a population of immature neurons in the piriform cortex. These neurons in the piriform cortex are generated during embryonic development, retain the expression of immaturity markers such as doublecortin, and slowly mature and integrate into the olfactory circuit as the animal ages. To study how early life experiences affect this population of cortical immature neurons, we submitted mice of the C57/Bl6J strain to a protocol of maternal separation for 3 h per day from postnatal day 3 to postnatal day 21. Control mice were continuously with their mothers. After weaning, mice were undisturbed until 6 weeks of age, when they were weighted and tested in the elevated plus-maze, a standard test for anxiety-like behavior, to check for phenotypical effects. Mice were then perfused, and their brains processed for the immunofluorescent detection of doublecortin and the endogenous proliferation marker Ki67. We found that maternal separation induced a significant increase in the body weight of males, but not females. Further, maternally separated mice displayed increased exploratory-like behavior (i.e., head dipping, velocity and total distance traveled in the elevated plus maze), but no significant differences in anxiety-like behavior or corticosterone levels after behavioral testing. Finally, we observed a significant increase in the number of complex doublecortin neurons in the piriform cortex, but not in the olfactory bulbs, of mice submitted to maternal separation. Interestingly, most doublecortin neurons in the piriform cortex, but not the olfactory bulb, express the epigenetic reader MeCP2. In summary, mild early life stress results, during adolescence, in a male-specific increase in body weight, alteration of the exploratory behaviors, and an increase in doublecortin neurons in the piriform cortex, suggesting an alteration in their maturation process.
Clinical significance of the modified Naples prognostic score in patients with stage II-III colon cancer undergoing curative resection: a retrospective study from the real world
Authors: Li Xiaopeng , Cheng Chen , Huo Xiongwei , Zhao Chenye , Yuan Hang , Chen Gang , Yu Junhui , Mu Mingchao , Sun Xuejun
Published: September 2024

Background

The Naples prognostic score (NPS) determined by the nutritional and inflammatory condition of an individual is attracting growing attention for predicting postoperative outcomes in a variety of malignancies. The study aimed to assess the clinical significance of a modified NPS (M-NPS) and establish and validate nomograms incorporating M-NPS in curative stage II-III colon cancer patients.

Methods

We retrospectively analyzed 328 stage II-III colon cancer patients receiving radical surgical resection at our hospital from January 2011 to December 2016. Kaplan–Meier (KM) survival analysis and Cox regression analysis were executed for overall survival (OS) and cancer-specific survival (CSS). Independent predictive indicators were applied to develop nomograms. The model’s performance was evaluated using many different methods.

Results

Of a total of 328 cases, 153 cases were in group 0, 145 in group 1, and 30 in group 2. In terms of OS or CSS, there were obvious differences between groups 0 and 1, and between groups 0 and 2. Age, obstruction, N stage, gross tumor type, and M-NPS group were independent prognostic indicators for OS, while obstruction, gross tumor type, M-NPS group, and N stage were independent predictive parameters for CSS. Furthermore, the training and validation sets were randomly allocated among a cohort of 328 patients. OS and CSS prediction nomograms were developed. In the training and validation cohort, the C-index and ROC analysis showed good discrimination, calibration curves exhibited an excellent level of consistency between model-predicted survival and actual survival outcomes, and DCA curves demonstrated good clinical performance.

Conclusion

M-NPS is a reliable survival predictor in patients with curative stage II-III colon cancer. Nomograms incorporating M-NPS for OS and CSS have good predictive performance and clinical utility.
Correlating dermatoscopic features with immunohistochemical markers in basal cell carcinoma: a comprehensive analysis of 100 cases in Caucasian population
Authors: Calik Jacek , Sauer Natalia , Giedziun Piotr , Piotrowska Aleksandra , Tumiłowicz Maciej , Wojnar Andrzej , Dzięgiel Piotr
Published: September 2024

Background

Basal Cell Carcinoma (BCC) is the most common form of skin cancer, characterized by its low metastatic potential yet considerable diversity in clinical and dermatoscopic presentation. Advances in dermatoscopy have significantly improved the early detection of BCC, revealing specific patterns that guide diagnosis and management. Parallelly, immunohistochemical markers have been explored for their potential to elucidate the underlying tumor biology and prognosis, with particular focus on angiogenesis, melanocytic activity, and lymphangiogenesis.

Objective

This study aims to investigate the correlations between dermatoscopic features and the immunohistochemical expressions of CD34, CD31, Melan-A, and D2-40 in BCC, through a comprehensive analysis of 100 cases We sought to determine whether visual dermatoscopic patterns correlate with the molecular characteristics defined by immunohistochemical staining, potentially enhancing diagnostic accuracy.

Methods

A total of 100 cases of clinically and histopathologically confirmed BCC were prospectively analyzed, employing standard dermatoscopic techniques for lesion evaluation and immunohistochemical staining for CD34, CD31, Melan-A, and D2-40 to assess tumor angiogenic potential, melanocytic activity, and lymphangiogenesis. The study was conducted with adherence to ethical standards and informed consent from all participants.

Results

Dermatoscopic examination revealed a variety of vascular patterns and pigmented features across different BCC anatomical locations. However, the comprehensive correlation analysis predominantly found a lack of significant associations between dermatoscopic appearances and expressions of the targeted immunohistochemical markers, with the notable exception of a correlation between observed hemorrhage and the Melan-A marker.

Conclusions

The lack of significant correlations between dermatoscopic features and immunohistochemical marker expressions in BCC suggests that the biological behavior and angiogenic, melanocytic, and lymphangiogenic activities within BCC lesions may be influenced by factors beyond those assessed in this study. Despite the exploratory nature of these findings, they underscore the complexity of BCC biology and highlight the need for further research incorporating additional markers and advanced imaging techniques.
Wandering spleen combined with pedicle torsion and splenic infarction: a rare case report and literature review
Authors: Cui Qian , Xu Xiaoyan , Li Chang , Tang Lihao
Published: September 2024

Wandering spleen (WS) concurrent with splenic pedicle torsion and infarction has been described rarely. We reported our experience in diagnosing and treating such a condition in a 16-year-old girl with acute abdominal pain. A plain CT scan showed the wandering of the spleen from the left upper quadrant. Contrast-enhanced CT indicated dilatation and distortion in the splenic vein, a counterclockwise “whirl sign” in the splenic pedicle, pancreatic tail torsion, and splenic infarction. The patient was diagnosed with WS combined with splenic pedicle torsion and splenic infarction and underwent splenectomy for treatment. She showed a satisfactory outcome during the follow-up. To enhance our understanding of it, we performed a comprehensive literature research to summarize the clinical manifestations, treatment options, and outcomes among adolescent patients.
Novel multitarget analgesic candidate SZV-1287 demonstrates potential disease-modifying effects in the monoiodoacetate-induced osteoarthritis mouse model
Authors: Horváth Ádám István , Bölcskei Kata , Szentes Nikolett , Borbély Éva , Tékus Valéria , Botz Bálint , Rusznák Kitti , Futácsi Anett , Czéh Boldizsár , Mátyus Péter , Helyes Zsuzsanna
Published: September 2024

Introduction

Monoiodoacetate (MIA)-induced osteoarthritis (OA) is the most commonly used rodent model for testing anti-OA drug candidates. Herein, we investigated the effects of our patented multitarget drug candidate SZV-1287 (3-(4,5-diphenyl-1,3-oxazol-2-yl) propanal oxime) that is currently under clinical development for neuropathic pain and characterized the mouse model through complex functional, in vivo imaging, and morphological techniques.

Methods

Knee OA was induced by intraarticular MIA injection (0.5 and 0.8 mg). Spontaneous pain was assessed based on weight distribution, referred pain by paw mechanonociception (esthesiometry), edema by caliper, neutrophil myeloperoxidase activity by luminescence, matrix metalloproteinase activity, vascular leakage and bone remodeling by fluorescence imaging, bone morphology by micro-CT, histopathological alterations by semiquantitative scoring, and glia activation by immunohistochemistry. Then, SZV-1287 (20 mg/kg/day) or its vehicle was injected intraperitoneally over a 21-day period.

Results

MIA induced remarkably decreased thresholds of weight bearing and paw withdrawal, alterations in the tibial and femoral structures (reactive sclerosis, increased trabeculation, and cortical erosions), histopathological damage (disorganized cartilage structure, hypocellularity, decreased matrix staining and tidemark integrity, and increased synovial hyperplasia and osteophyte formation), and changes in the astrocyte and microglia density in the lumbar spinal cord. There were no major differences between the two MIA doses in most outcome measures. SZV-1287 inhibited MIA-induced weight bearing reduction, hyperalgesia, edema, myeloperoxidase activity, histopathological damage, and astrocyte and microglia density.

Conclusion

SZV-1287 may have disease-modifying potential through analgesic, anti-inflammatory, and chondroprotective effects. The MIA mouse model is valuable for investigating OA-related mechanisms and testing compounds in mice at an optimal dose of 0.5 mg.
Study on pharmacokinetic and tissue distribution of hyperin, astragalin, kaempferol-3-O-β-D-glucuronide from rats with multiple administrations of Semen Cuscutae processed with salt solution with effect of treating recurrent spontaneous abortion
Authors: Yang Zhitong , Chen Kaiwen , Zhang Yuting , Xu Baiyang , Huang Yu , Zhang Xue , Liu Zilu , Wang Tongsheng , Wu Deling , Peng Tangyi , Lu Tulin , Cai Hao , Wang Xiaoli
Published: September 2024

Introduction

Semen Cuscutae is a traditional Chinese medicine (TCM) that tonifies the kidneys and prevents miscarriage. According to Chinese medicine theory, kidney deficiency is one of the main causes of recurrent spontaneous abortion (RSA). The previous studies showed that raw product of Semen Cuscutae (SP) and Semen Cuscutae processed with salt solution (YP) have ameliorative effects on RSA, and that YP is superior to SP. However, the active components of YP to ameliorate RSA remain unclear and require further studies. The objective of this study is to investigate the active components of YP in ameliorating RSA.

Methods

First, a rat model of RSA was established using hydroxyurea in combination with mifepristone. Aqueous decoction of YP was given by gavage to rats. Second, pregnant rats were sampled on days 5, 7, 9, 10 and 12 during the modelling period. The content of Hyperin (HY), astragalin (AS) and kaempferol-3-O-β-D-glucuronide (KA) in blood and liver, heart, spleen, lung and kidney tissues were detected by liquid chromatography-mass spectrometry (LC-MS). The pharmacodynamic indicators including progesterone (P), chorionic gonadotropin β (β-HCG), estradiol (E2), tumor necrosis factor-α (TFN-α), interleukin 4 (IL-4), and tryptophan (TRP) were measured by enzyme-linked immunosorbent assay (ELISA) Pearson's correlation analysis and grey relational analysis were used to establish the relationship between the pharmacodynamic indexes and chemical constituents.

Results

The pharmacokinetic results showed that the area under curve (AUC) value of KA was the largest. The tissue distribution results showed that astragalin was widely distributed in liver, heart, spleen, lung and kidney in the RSA model rats, while HY was detected only in the uterus, and KA was detected only in the kidney. The pearson correlationl analysis showed that KA was significantly and positively correlated with the contents of E2, P, β-HCG and TRP. Both AS and HY were significantly negatively correlated with the content of TNF-α, respectively.

Discussion

This study reveals the pharmacokinetics and tissue distribution of KA, AS and HY in rats with RSA. It was elucidated that all three were involved in the regulation of progesterone levels and immune function. It initially revealed the mechanism of action of YP in enhancing the improvement of RSA, and it provided a theoretical basis for the quality assessment of YP.
Oleuropein enhances proteasomal activity and reduces mutant huntingtin-induced cytotoxicity
Authors: Huang Zih-Ning , Lee Sin-Yi , Chen Jie-Mao , Huang Zih-Ting , Her Lu-Shiun
Published: September 2024

Introduction

Huntington’s disease (HD) is a hereditary neurodegenerative disorder that primarily affects the striatum, a brain region responsible for movement control. The disease is characterized by the mutant huntingtin (mHtt) proteins with an extended polyQ stretch, which are prone to aggregation. These mHtt aggregates accumulate in neurons and are the primary cause of the neuropathology associated with HD. To date, no effective cure for HD has been developed.

Methods

The immortalized STHdhQ111/Q111 striatal cell line, the mHtt-transfected wild-type STHdhQ7/Q7 striatal cell line, and N2a cells were used as Huntington's disease cell models. Flow cytometry was used to assess cellular reactive oxygen species and transfection efficiency. The CCK-8 assay was used to measure cell viability, while fluorescence microscopy was used to quantify aggregates. Immunoblotting analyses were used to evaluate the effects on protein expression.

Results

Polyphenols are natural antioxidants that offer neuroprotection in neurological disorders. In this study, we provide evidence that oleuropein, the primary polyphenol in olive leaves and olive oil, enhances cell viability in HD cell models, including. STHdhQ7/Q7STHdhQ7/Q7 striatal cells, N2a cells ectopically expressing the truncated mHtt, and STHdhQ111/Q111 striatal cells expressing the full-length mHtt. Oleuropein effectively reduced both soluble and aggregated forms of mHtt protein in these HD model cells. Notably, the reduction of mHtt aggregates associated with oleuropein was linked to increased proteasome activity rather than changes in autophagic flux. Oleuropein seems to modulate proteasome activity through an unidentified pathway, as it did not affect the 20S proteasome catalytic β subunits, the proteasome regulator PA28γ, or multiple MAPK pathways.

Discussion

We demonstrated that oleuropein enhances the degradation of mHtt by increasing proteasomal protease activities and alleviates mHtt-induced cytotoxicity. Hence, we propose that oleuropein and potentially other polyphenols hold promise as a candidate for alleviating Huntington's disease.
Integrated GWAS, linkage, and transcriptome analysis to identify genetic loci and candidate genes for photoperiod sensitivity in maize
Authors: Jiang Yulin , Guo Shuang , Wang Dong , Tu Liang , Liu Pengfei , Guo Xiangyang , Wang Angui , Zhu Yunfang , Lu Xuefeng , Chen Zehui , Wu Xun
Published: September 2024

Introduction

Maize photosensitivity and the control of flowering not only are important for reproduction, but also play pivotal roles in the processes of domestication and environmental adaptation, especially involving the utilization strategy of tropical maize in high-latitude regions.

Methods

In this study, we used a linkage mapping population and an inbred association panel with the photoperiod sensitivity index (PSI) phenotyped under different environments and performed transcriptome analysis of T32 and QR273 between long-day and short-day conditions.

Results

The results showed that PSIs of days to tasseling (DTT), days to pollen shedding (DTP), and days to silking (DTS) indicated efficacious interactions with photoperiod sensitivity for maize latitude adaptation. A total of 48 quantitative trait loci (QTLs) and 252 quantitative trait nucleotides (QTNs) were detected using the linkage population and the inbred association panel. Thirteen candidate genes were identified by combining the genome-wide association study (GWAS) approach, linkage analysis, and transcriptome analysis, wherein five critical candidate genes, MYB163, bif1, burp8, CADR3, and Zm00001d050238, were significantly associated with photoperiod sensitivity.

Discussion

These results would provide much more abundant theoretical proofs to reveal the genetic basis of photoperiod sensitivity, which would be helpful to understand the genetic changes during domestication and improvement and contribute to reducing the barriers to use of tropical germplasm.
The relationship of academic procrastination on non-English majors’ English classroom anxiety: a moderated mediation model
Authors: Jiang Feng
Published: September 2024

Objective

Academic procrastination is negatively associated with English classroom anxiety among non-English major college students. However, current research has less explored the underlying mechanisms. The present study aims to investigate the relationship between academic procrastination and college students’ English classroom anxiety as well as the mediating role of learning engagement and the moderating role of appraisals of intrinsic values.

Methods

The academic procrastination scale, English classroom anxiety scale, learning engagement scale and appraisals of intrinsic values scale were used to measure 1,079 non-English majors in Jiangsu Province, China.

Results

(1) There was a significant positive correlation between academic procrastination and English classroom anxiety of non-English majors. (2) Learning engagement plays a part of mediating role between academic procrastination and English classroom anxiety; (3) When the appraisals of intrinsic values are high, the impact effect of learning engagement on English classroom anxiety is greater.

Conclusion

Academic procrastination can affect university students’ English classroom anxiety through learning engagement, and this relational pattern is modulated by appraisals of intrinsic values. This finding provides an important theoretical basis and practical insights for understanding and intervening in academic procrastination and classroom anxiety among college students.
Understanding “Childhood Poly-Victimization” to help uncover abuse during child investigative interviewing: a systematic review
Authors: Morlat Pantxika Victoire , Alison Laurence
Published: September 2024

Introduction

It is well established that child victims are some of the most challenging populations to interview. Indeed, children tend to feel ashamed, scared or in denial, leading to difficulties for law enforcement when gathering information, and subsequently with prosecuting offenders. Moreover, with crimes against children increasing, it is common for interviewed victims to have experienced several abuses (poly-victimization). Thus, the main goals of this study are to (1) identify the facets that are included in poly-victimization, in order to (2) provide a clear definition that can be used by law enforcement during child interviews, which could lead to (3) a better identification of cases of abuse to maximize safeguarding and protect children.

Materials and methods

A systematic review was used to establish the differences in conceptualizations of poly-victimization as well as the measurements chosen by research to measure this concept. The current systematic review included research articles on childhood poly-victimization using a quantitative measuring instrument published as of 2007, that focused on populations under 18 years of age.

Results

The findings were divided into (1) conceptual dimensions, (2) characteristics of studies’ populations and (3) measures and psychometrics properties. It was found that research used various terms for poly-victimization, yet with numerous definitions implying differences in number of abuses, time frame, and mental health aspects to be considered in the identification of poly-victimization. The included papers (n = 6) were from Europe, Asia, and the United States. Over half of the studies used the Juvenile Victimization Questionnaire (JVQ) but adapted either (i) to the study’s population, (ii) possible answers, (iii) wording of questions, or (iv) by the removal of questions.

Conclusion

Our findings highlight the need for a more theoretically coherent definition of childhood poly-victimization. Questions regarding number of abuses, time consideration (past year vs. lifetime), mental health and severity of abuse should be addressed to develop a unified definition of poly-victimization. Rapport-based interviews should be the focus to uncover the truth and to avoid a secondary trauma in the crisis of a child’s disclosure of abuse. A new definition of “Childhood Poly-Victimization” is proposed by the authors as well as a “Decision Tree for Identifying Childhood Poly-Victimization” designed to be used by law enforcement during child investigative interviews.
Research on designers’ behavioral intention toward Artificial Intelligence-Aided Design: integrating the Theory of Planned Behavior and the Technology Acceptance Model
Authors: Jiao Jinchuan , Cao Xiangnan
Published: September 2024

Artificial Intelligence-Aided Design (AIAD) has numerous advantages and tremendous benefits for designers. However, not all designers are keen to integrate AIAD into their workflow, and their intention to use AIAD remains a research gap. This study explores designers’ adoption of AIAD, utilizing the Theory of Planned Behavior (TPB) and the Technology Acceptance Model (TAM). Drawing on extant literature, we proposed a research model and tested it using data from 392 Chinese designers. The results indicate that in terms of AIAD, (a) designers’ attitudes toward AIAD (b = 0.259, p < 0.001), subjective norms (b = 0.363, p < 0.001), and perceived behavioral control (b = 0.556, p < 0.001) have significant and positive impacts on their intention to use AIAD; (b) perceived usefulness of AIAD (b = 0.910, p < 0.001) has a positive and significant correlation with attitudes toward AIAD while perceived ease of use (b = −0.126, p < 0.05) exerts no significant impact on attitudes; (c) the knowledge level of designers (b = −0.149, p < 0.01) has a negative moderating effect on the impact of attitudes toward AIAD on the intention to use them. The present research then discusses its practical significance.
Making sense of a pandemic: reasoning about COVID-19 in the intellectual dark web
Authors: Doody Sean
Published: September 2024

In this study, I examine how users of an online Reddit community, r/IntellectualDarkWeb, forged an anti-establishment collective identity through practices of “heterodox scientific” reasoning. I do so through a discursive analysis of comments and posts made to r/IntellectualDarkWeb during the COVID-19 pandemic. First, I deploy the BERTopic algorithm to cluster my corpus and surface topics pertaining to COVID-19. Second, I engage in a qualitative content analysis of the relevant clusters to understand how discourses about COVID-19 were mobilized by subreddit users. I show that discussions about COVID-19 were polarized along “contrarian” and “anti-contrarian” lines, with significant implications for the subreddit’s process of collective identity. Overwhelmingly, contrarian content that expressed skepticism towards vaccines, mistrust towards experts, and cynicism about the medical establishment was affirmed by r/IntellectualDarkWeb users. By contrast, anti-contrarian content that sought to counter anti-vaccine rhetoric, defend expertise, or criticize subreddit users for their contrarianism was penalized. A key factor in this dynamic was Reddit’s scoring mechanism, which empowered users to publicly upvote contrarian affirming content while simultaneously downvoting anti-contrarian content. As users participated in sense making about COVID-19, they deployed Reddit’s scoring mechanism to reinforce a contrarian collective identity oriented around a practice of heterodox science. My research shows the continued relevance of the concept of collective identity in the digital age and its utility for understanding contemporary reactionary social movements.
Phosphorus availability from olivine for biospheres
Authors: Abbatiello Josh , Henson John E. , Pasek Matthew
Published: September 2024

Phosphorus plays a multifaceted role for all known life and hence understanding its sources on the early Earth provides constraints on how life developed to incorporate this element into its biochemistry. Currently, the major phosphorus mineral group on the surface of the Earth are the apatites, which are poorly soluble calcium phosphates and hence may not have been a good source of phosphorus on the early Earth. An alternative source of phosphorus may be the mineral olivine. Given that olivine makes up a large part of the upper mantle of Earth and presumably other rocky planets and moons, it stands to reason that olivine may be a potential phosphorus reservoir for prebiotic chemical environments. Here we examine the phosphorus content of 10 olivine samples from different terrestrial localities to determine their P content and P speciation. We find that extracts of the samples contain varying amounts of phosphate, and some contain pyrophosphate. Olivine may have served as a source of phosphate on the early Earth and possibly elsewhere in the solar system, and its dissolution could have supplied this nutrient to a nascent biosphere.
Transanal intersphincteric under direct view in the jackknife position combined with laparoscopic total mesorectal excision for treating ultra-low rectal cancer
Authors: Zhong Guobin , Chen Zhiyu , Li Zhenfeng , Zhao Bin , Deng Junhui
Published: September 2024

Aim

To investigate the effect and clinical advantage of transanal intersphincteric (ISR) under direct view in the jackknife position combined with laparoscopic total mesorectal excision (TME) for treating ultra-low rectal cancer. Additionally, the feasibility of this surgical technique was evaluated.

Method

This was a retrospective, single-center, single-arm pilot study. Ten patients with ultra-low rectal cancer underwent treatment by the same surgical team for direct view transanal ISR combined with laparoscopic TME in the Department of Anorectal Surgery, Huizhou Central People's Hospital between January 2021 and June 2021. The relevant clinical data were collected and analyzed.

Results

All the patients underwent complete mesenteric resection without conversion to laparotomy. The circumferential and distal resection margins (CRM and DRM) were negative. The mean distance between the lower margin of the tumor and the anal margin was 2.8 ± 0.8 cm, and the mean margin of distal resection was 1.2 ± 0.2 cm. TNM pathological stages I, II, III, and IV were observed in 6, 2, 2, and 0 cases, respectively. The median follow-up period was 15 months (interquartile range, 8 months). The mean Wexner and Low Anterior Resection Syndrome scores at 12 months after ileostomy were 8.1 ± 2.1 and 22.4 ± 5.7, respectively.

Conclusion

Transanal ISR under direct view in the jackknife position combined with laparoscopic TME is safe and feasible for the treatment of ultralow rectal cancer.
Effect of coated-benzoic acid on growth performance, immunity, and intestinal functions in weaned pigs challenged by enterotoxigenic Escherichia coli
Authors: Qi Jiawen , Yu Bing , Hu Youjun , Luo Yuheng , Zheng Ping , Mao Xiangbing , Yu Jie , Zhao Xiaonan , He Taiqian , Yan Hui , Wu Aimin , He Jun
Published: September 2024

Introduction

Benzoic acid (BA) could be added to the diets of weaned pigs to prevent diarrhea due to its antibacterial function. However, BA may be absorbed or decomposed before it can reach the hindgut. This study was conducted to explore the effect of a novel coated benzoic acid (CBA) on growth performance, immunity, and intestinal barrier functions in weaned pigs upon enterotoxigenic Escherichia coli (ETEC) challenge.

Methods

In a 21d experiment, 32 piglets were randomly assigned to 4 treatments: (1) a basal diet (CON), (2) CON added with CBA at 3  g/kg (CBA); (3) CON and challenged by ETEC (ECON); (4) CON added with CBA at 3 g/kg and challenged by ETEC (ECON). On d 22, all piglets were euthanised to obtain samples.

Results

Dietary CBA supplementation elevated the average daily gain (ADG) of the ETEC-challenged pigs (p < 0.05). CBA also improved the digestibility of dry matter, gross energy, and ash (p < 0.05). Moreover, CBA elevated the ratio of blood basophil and the serum concentration of total cholesterol of the ETEC challenged pigs (p < 0.05). Importantly, CBA increased the serum concentrations of immunoglobulin A (IgA), IgG, and IgM (p < 0.05). CBA not only decreased the crypt depth but also increased the ratio of villus height to crypt depth (V:C) in the jejunum and ileum (p < 0.05). Moreover, CBA increased the activities of jejunal and ileal sucrase, and the activities of duodenal and ileal maltase (p < 0.05). Importantly, CBA elevated the expression levels of critical functional genes such as the claudin-1, occluding, glucose transporter-2 (GLUT2), and sodium/glucose cotransporter-1 (SGLT-1) in the jejunal epithelium upon ETEC challenge (p < 0.05). Additionally, CBA increased the abundances of total bacteria and Bacillus, and increased the concentrations of volatile fatty acids (acetic acid, propanoic acid, and butyric acid) in cecum (p < 0.05).

Discussion

These results suggested a beneficial role for CBA in alleviating intestinal injury in weaned pigs following ETEC challenge. Such effects may be tightly associated with elevated immunity and improved intestinal epithelium functions and microbiota.
Association Model-Based Intermittent Connection Fault Diagnosis for Controller Area Networks
Authors: Wang Longkai , Hu Shuqi , Lei Yong
Published: September 2024

Controller Area Networks (CANs) play an important role in many safety-critical industrial systems, which places high demands on their reliability performance. However, the intermittent connection (IC) of network cables, a random and transient connectivity problem, is a common but hard troubleshooting fault that can cause network performance degradation, system-level failures, and even safety issues. Therefore, to ensure the reliability of CANs, a fault symptom association model-based IC fault diagnosis method is proposed. Firstly, the symptoms are defined by examining the error records, and the domains of the symptoms are derived to represent the causal relationship between the fault locations and the symptoms. Secondly, the fault probability for each location is calculated by minimizing the difference between the symptom probabilities calculated from the count information and those fitted by the total probability formula. Then, the fault symptom association model is designed to synthesize the causal and the probabilistic diagnostic information. Finally, a model-based maximal contribution diagnosis algorithm is developed to locate the IC faults. Experimental results of three case studies show that the proposed method can accurately and efficiently identify various IC fault location scenarios in networks.
Preferences of Generations of Customers in Slovakia in the Field of Marketing Communication and Their Impact on Consumer Behaviour
Authors: Cagala Matúš , Babčanová Dagmar
Published: September 2024

This paper deals with the analysis of the preferences of different customer generations in Slovakia in the field of marketing communication and its influence on consumer behaviour. Currently, marketing communication is an integral part of company strategies and has a key influence on how customers choose products or services. With the increasing importance of the generational segmentation of customers, understanding their individual preferences and tendencies in communication becomes an essential prerequisite for success. In this paper, we explore how preferences differ between Baby Boomers, Generation X, Generation Y, and Generation Z, and how these preferences influence their consumer behaviour. Based on the analysis of primary and secondary data, the main trends and preferences of generations in the field of marketing communication were identified. This paper provides useful information that can be used by companies in the creation and implementation of marketing strategies. At the same time, the paper enables a better understanding of the dynamics of consumer behaviour in the context of generational preferences and will help identify opportunities for improving marketing activities and more effective communication with target groups.
Photosynthetic Performance and Heterogeneous Anatomical Structure in Prunus humilis under Saline–Alkaline Stress
Authors: Sun Yongjiang , Wang Xiang , Shao Qiwen , Wang Qi , Wang Siyuan , Yu Ruimin , Dong Shubin , Xin Zhiming , Xiao Huijie , Cheng Jin
Published: September 2024

Prunus (P.) humilis is a small woody shrub that has been widely planted in northern China due to its high nutritional value and resistance to environmental abiotic stress. However, little information about the responses of photosynthetic performance and the anatomical structure of P. humilis to saline–alkaline stress (SAS) under field conditions is available. Here, we investigated the behavior of the photosynthetic apparatus of P. humilis by measuring the chlorophyll fluorescence parameters under moderate (MS) and severe (SS) saline–alkaline stress and analyzing their relationship to leaf anatomical traits. The results showed that SAS significantly decreased the net photosynthetic rate (An) but increased the substomatal CO2 concentration (Ci). The maximum photochemical quantum yield of PSII (Fv/Fm) and the efficient quantum yield of PSII [Y(II)] decreased under MS and SS conditions, and this decrease was greater in the distal (tip) than in the proximal (base) leaf. Compared to the leaf tip, the base of P. humilis leaves seemed to have a stronger ability to cope with MS, as was made evident by the increased quantum yield of regulated energy dissipation in PSII [Y(NPQ)] and decreased excitation pressure (1-qP). Under MS and SS conditions, the shapes of the chlorophyll a fluorescence transient (OJIP) changed markedly, accompanied by decreased PSII acceptor-side and donor-side activities. The palisade–spongy tissue ratio (PT/ST) increased significantly with increasing stress and showed a significant correlation with the chlorophyll fluorescence parameters in the leaf base. These results suggested that the activity of PSII electron transfer in the upper leaf position tended to be more sensitive to saline–alkaline stress, and a chlorophyll fluorescence analysis proved to be a good technique to monitor impacts of saline–alkaline stress on photosynthetic function, which may reflect the non-uniformity of leaf anatomy. In addition, among the anatomical structure parameters, the palisade–spongy tissue ratio (PT/ST) can be used as a sensitive indicator to reflect the non-uniform of photosynthetic function and leaf anatomy under stress.
Direct Conversion of Minimally Pretreated Corncob by Enzyme-Intensified Microbial Consortia
Authors: Geng Alei , Li Nana , Zayas-Garriga Anaiza , Xie Rongrong , Zhu Daochen , Sun Jianzhong
Published: September 2024

The presence of diverse carbohydrate-active enzymes (CAZymes) is crucial for the direct bioconversion of lignocellulose. In this study, various anaerobic microbial consortia were employed for the degradation of 10 g/L of minimally pretreated corncob. The involvement of lactic acid bacteria (LAB) and a CAZyme-rich bacterium (Bacteroides cellulosilyticus or Paenibacillus lautus) significantly enhanced the lactic acid production by Ruminiclostridium cellulolyticum from 0.74 to 2.67 g/L (p < 0.01), with a polysaccharide conversion of 67.6%. The supplement of a commercial cellulase cocktail, CTec 2, into the microbial consortia continuously promoted the lactic acid production to up to 3.35 g/L, with a polysaccharide conversion of 80.6%. Enzymatic assays, scanning electron microscopy, and Fourier transform infrared spectroscopy revealed the substantial functions of these CAZyme-rich consortia in partially increasing enzyme activities, altering the surface structure of biomass, and facilitating substrate decomposition. These results suggested that CAZyme-intensified consortia could significantly improve the levels of bioconversion of lignocellulose. Our work might shed new light on the construction of intensified microbial consortia for direct conversion of lignocellulose.
Organic Capillary Barriers for Soil Water Accumulation in Agriculture: Design, Efficiency and Stability
Authors: Smagin Andrey , Sadovnikova Nadezhda , Krivtsova Victoria , Korchagina Christina , Krasilnikov Pavel
Published: September 2024

Acute shortage of water resources and high unproductive water losses are the key problems of irrigated agriculture in arid regions. One of the possible solutions is to optimize soil water retention using natural and synthetic polymer water absorbers. Our approach uses the HYDRUS-1D design to optimize the placement of organic water absorbents such as peat and composite hydrogels in the soil profile in the form of water-storing capillary barriers. Field testing of the approach used a water balance greenhouse experiment with the cultivation of butternut squash (butternut squash (Cucurbita moschata (Duchesne, 1786)) under sprinkler irrigation with measurement of the soil moisture profile and unproductive water losses in the form of lysimetric water outflow. In addition, the biodegradation rate of organic water absorbents was studied at the soil surface and at a depth of 20 cm. Organic capillary barriers reduced unproductive water losses by 40–70%, retaining water in the topsoil and increasing evapotranspiration by 70–130% with a corresponding increase in plant biomass and fruit yield. The deepening of organic soil modifiers to the calculated depth not only allowed capillary barriers to form, but also prevented their biodegradation. The best results in soil water retention, plant growth and yield according to the “dose-effect” criterion were obtained for a composite superabsorbent with peat filling of an acrylic polymer matrix. The study showed good compliance between the HYDRUS design and the actual efficiency of capillary barriers as an innovative technology for irrigated agriculture using natural and synthetic water absorbents.
Exogenous Substances Improved Salt Tolerance in Cotton
Authors: Dong Zhiduo , Meng Ajing , Qi Tong , Huang Jian , Yang Huicong , Tayir Aziguli , Wang Bo
Published: September 2024

Soil salinization is a major limiting factor for cotton growth in Southern Xinjiang. Studying technologies and mechanisms to improve cotton salt tolerance is of significant importance for the development and utilization of saline–alkaline land. In this study, ‘Xinluzhong 40’ cotton was used as the material, and 150 mmol·L−1 sodium chloride (NaCl) and 1.2% natural saline–alkaline soil extract were employed to simulate single-salt (SS) and mixed-salt (MS) stresses, respectively. The effects of different exogenous substances (sodium nitrophenolate, 24-epibrassinolide, and γ-aminobutyric acid) on the growth characteristics of cotton under salt stress were investigated. The results show that: (1) Under salt stress, the height and biomass of cotton (50 d old) were reduced. Both SS and MS stresses led to increased superoxide dismutase (SOD) activity, elevated proline (PRO) content (with an increase of 50.01% and no significant difference), and increased malondialdehyde (MDA) content (with increases of 63.14% and 32.42%, respectively). At the same time, catalase (CAT) activity decreased, Na+ and Cl− contents increased, K+ content decreased, and the K+/Na+ ratio was reduced. (2) Application of sodium nitrophenolate (S), 24-epibrassinolide (E), and γ-aminobutyric acid (G) significantly improved SOD activity and PRO content while reducing MDA content (decreased by 29.33%, 25.48%, and 30.47% compared to SS treatment; and 1.68%, 5.21%, and 5.49% compared to MS treatment, respectively). They also increased CAT activity (increased by 75.97%, 103.24%, and 80.79% compared to SS treatment; and 91.06%, 82.43%, and 119.68% compared to MS treatment, respectively) and K+/Na+ ratio (increased by 57.59%, 66.35%, and 70.50% compared to SS treatment; and 38.31%, 42.97%, and 66.66% compared to MS treatment, respectively), reduced Cl− content, and promoted increases in plant height and biomass. The effects of exogenous substances on antioxidant capacity and ion balance under salt stress were significant, particularly under SS stress. (3) Principal component analysis revealed that under SS and MS stresses, principal component 1 mainly reflects cotton’s antioxidant capacity, with SOD, CAT, and PRO having high weights; principal component 2 mainly reflects cotton’s ion balance and nutrient absorption, with root Na+, stem Na+, leaf Na+, root K+, and root Cl− having high weights. These findings highlight the potential of exogenous substances to improve cotton salt tolerance and provide scientific evidence for cotton cultivation on saline–alkaline land, offering new insights into cultivation techniques from an applied research perspective.