bluuebunny/crossref_metadata_2025 huggingface dataset:
Oligonucleotides and microRNAs Targeting Telomerase Subunits in Cancer Therapy
Authors: Eckburg Adam , Dein Joshua , Berei Joseph , Schrank Zachary , Puri Neelu
Published: August 2020

Telomerase provides cancer cells with replicative immortality, and its overexpression serves as a near-universal marker of cancer. Anti-cancer therapeutics targeting telomerase have garnered interest as possible alternatives to chemotherapy and radiotherapy. Oligonucleotide-based therapies that inhibit telomerase through direct or indirect modulation of its subunits, human telomerase reverse transcriptase (hTERT) and human telomerase RNA gene (hTERC), are a unique and diverse subclass of telomerase inhibitors which hold clinical promise. MicroRNAs that play a role in the upregulation or downregulation of hTERT and respective progression or attenuation of cancer development have been effectively targeted to reduce telomerase activity in various cancer types. Tumor suppressor miRNAs, such as miRNA-512-5p, miRNA-138, and miRNA-128, and oncogenic miRNAs, such as miRNA-19b, miRNA-346, and miRNA-21, have displayed preclinical promise as potential hTERT-based therapeutic targets. Antisense oligonucleotides like GRN163L and T-oligos have also been shown to uniquely target the telomerase subunits and have become popular in the design of novel cancer therapies. Finally, studies suggest that G-quadruplex stabilizers, such as Telomestatin, preserve telomeric oligonucleotide architecture, thus inhibiting hTERC binding to the telomere. This review aims to provide an adept understanding of the conceptual foundation and current state of therapeutics utilizing oligonucleotides to target the telomerase subunits, including the advantages and drawbacks of each of these approaches.
Identification of Novel Fusion Genes in Bone and Soft Tissue Sarcoma and Their Implication in the Generation of a Mouse Model
Authors: Teramura Yasuyo , Tanaka Miwa , Yamazaki Yukari , Yamashita Kyoko , Takazawa Yutaka , Ae Keisuke , Matsumoto Seiichi , Nakayama Takayuki , Kaneko Takao , Musha Yoshiro , Nakamura Takuro
Published: August 2020

Fusion genes induced by chromosomal aberrations are common mutations causally associated with bone and soft tissue sarcomas (BSTS). These fusions are usually disease type-specific, and identification of the fusion genes greatly helps in making precise diagnoses and determining therapeutic directions. However, there are limitations in detecting unknown fusion genes or rare fusion variants when using standard fusion gene detection techniques, such as reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization (FISH). In the present study, we have identified 19 novel fusion genes using target RNA sequencing (RNA-seq) in 55 cases of round or spindle cell sarcomas in which no fusion genes were detected by RT-PCR. Subsequent analysis using Sanger sequencing confirmed that seven out of 19 novel fusion genes would produce functional fusion proteins. Seven fusion genes detected in this study affect signal transduction and are ideal targets of small molecule inhibitors. YWHAE-NTRK3 expression in mouse embryonic mesenchymal cells (eMCs) induced spindle cell sarcoma, and the tumor was sensitive to the TRK inhibitor LOXO-101 both in vitro and in vivo. The combination of target RNA-seq and generation of an ex vivo mouse model expressing novel fusions provides important information both for sarcoma biology and the appropriate diagnosis of BSTS.
Diversity in the Extracellular Vesicle-Derived Microbiome of Tissues According to Tumor Progression in Pancreatic Cancer
Authors: Jeong Jin-Yong , Kim Tae-Bum , Kim Jinju , Choi Hwi Wan , Kim Eo Jin , Yoo Hyun Ju , Lee Song , Jun Hye Ryeong , Yoo Wonbeak , Kim Seokho , Kim Song Cheol , Jun Eunsung
Published: August 2020

This study was conducted to identify the composition and diversity of the microbiome in tissues of pancreatic cancer and to determine its role. First, extracellular vesicles (EVs) were obtained from the paired tumor and normal tissues, and 16s rRNA gene sequencing was performed. We identified the microbiomes, compared the diversity between groups, and found that Tepidimonas was more abundant in tumors. Second, larger tumors resulted in lower levels of Leuconostoc and Sutterella, and increased lymph node metastasis resulted in higher levels of Comamonas and Turicibacter in tumor tissues. Moreover, in the case of tumor recurrence, the levels of Streptococcus and Akkermansia were decreased in tumor tissues. Finally, with the supernatant of Tepidimonasfonticaldi, proliferation and migration of cells increased, and epithelial-mesenchymal transition and the Tricarboxylic Acid (TCA) cycle-related metabolites were enhanced. The composition and diversity of EV-derived microbiomes are important for providing novel insights into theragnostic approaches in pancreatic cancer.
Evolving Cognitive Dysfunction in Children with Neurologically Stable Opsoclonus–Myoclonus Syndrome
Authors: Goh En Lin , Scarff Kate , Satariano Stephanie , Lim Ming , Anand Geetha
Published: August 2020

Cognitive and acquired neurodevelopmental deficits have been reported in children with opsoclonus–myoclonus syndrome (OMS) and are known to be associated with more severe and relapsing disease course. However, there is a paucity of data regarding cognitive dysfunction in children with stable neurological disease. We report three children with OMS and evolving cognitive dysfunction in the context of a mild disease course. The children’s ages at disease onset were between 17 and 35 months and they were followed up for 4–10 years. Neuroblastoma was identified in one child. OMS severity scores ranged between 8 and 12/15 at presentation. They underwent immunotherapy and all were in remission by 7 months (range 4–13 months), with treatment maintained for 1 year. One child remained relapse-free, while two others had one clinical relapse each and were immunotherapy-responsive again. In all cases, evolving cognitive dysfunction was reported despite being in remission and stable off treatment for a median of 20 months (range of 12–31 months; two OMS scores of 0/15 and one of 2/15). In children with OMS who have completed treatment and have made full or near full neurological recovery, concerns remain regarding long-term outcome in terms of future learning and cognitive development.
Growing Season Air mass Equivalent Temperature (TE) in the East Central USA
Authors: Na-Yemeh Dolly , Mahmood Rezaul , Goodrich Gregory , Younger Keri , Cary Kevin , Durkee Joshua
Published: August 2020

Equivalent temperature (TE), which incorporates both dry (surface air temperature, T) and moist heat content associated with atmospheric moisture, is a better indicator of overall atmospheric heat content compared to T alone. This paper investigates the impacts of different types of air masses on TE during the growing season (April–September). The study used data from the Kentucky Mesonet for this purpose. The growing season was divided into early (April–May), mid (June–July), and late (August–September). Analysis suggests that TE for moist tropical (MT) air mass was as high as 61 and 81 °C for the early and mid-growing season, respectively. Further analysis suggests that TE for different parts of the growing seasons were statistically significantly different from each other. In addition, TE for different air masses was also statistically significantly different from each other. The difference between TE and T (i.e. TE-T) is smaller under dry atmospheric conditions but larger under moist conditions. For example, in Barren County, the lowest difference (20–10 °C) was 10 °C. It was reported on 18 April 2010, a dry weather day. On the other hand, the highest difference for this site was 48 °C and was reported on 11 August 2010, a humid day.
Finite Element Method for Thin Film Corrosion Modelling: Where We Advanced and Where We would like to Advance?
Authors: Saeedikhani Mohsen , Blackwood Daniel John
Published: August 2020

Thin film corrosion is a serious issue in almost every sector. Thus, simulation of corrosion under thin electrolyte films has always been of high interest as experimental studies are often challenging. Thus far, progress has been made to model the effect of several important factors on thin film corrosion rates. Some of these parameters are electrolyte thickness, electrolyte composition, chemical reactions in the electrolyte, electrode size and change in electrode size, environmental parameters, and corrosion products deposition. However, these parameters are mainly drawn from different studies and have not been modelled concurrently in a single simulation study, making the thin film corrosion model far from being complete yet. Therefore, despite the many efforts so far, thin film corrosion modelers still strive to push the modelling edges further. This paper takes into account some of the highlighted recent advances in thin film corrosion modelling based on the mentioned parameters to provide a perspective on not only how far the field has come, but also how far it still is from a complete thin film corrosion model. Discussions have also been made on future needs and prospects to advance the thin film corrosion models further.
Forbidden Reflections in TeO2 in the Vicinity of the Te L1 Absorption Edge
Authors: Ovchinnikova Elena , Novikov Dmitri , Zschornak Matthias , Kulikov Anton , Kozlovskaya Ksenia , Dmitrienko Vladimir , Oreshko Alexey , Blagov Alexander , Mukhamedzhanov Enver , Marchenkov Nikita , Borisov Mickhail , Khadiev Azat , Petrenko Arsen , Pisarevsky Yury
Published: August 2020

Examining forbidden reflections provides valuable information on electronic states and the local environment of resonant atoms in crystals. Experimental studies of two forbidden reflections 002 and 100 in TeO2 single crystals were performed at photon energies close to the L1 tellurium absorption edge. It was found that the spectral form corresponding to these two reflections looks almost identical, which is completely unexpected for a highly anisotropic material. Theoretical consideration shows that only one component fxy of the tensor describing dipole-dipole resonance scattering contributes to the 002 reflection, while two components fxy and fxz correspond to the 100 reflection. Numerical calculations show that the latter tensor component is comparable to the first one, but the combination of several geometric factors leads to the fact that its contribution to the spectrum is negligible. This explains the experimentally observed results. The finding shows a way for targeted investigation of single tensor components and makes it possible to compare different spectra and use them the study the physical phenomena in functional materials.
σ- and π-Hole Interactions
Authors: Frontera Antonio
Published: August 2020

Supramolecular chemistry is a very active research field that was initiated in the last century [...]
Automatic Diabetic Retinopathy Grading via Self-Knowledge Distillation
Authors: Luo Ling , Xue Dingyu , Feng Xinglong
Published: August 2020

Diabetic retinopathy (DR) is a common fundus disease that leads to irreversible blindness, which plagues the working-age population. Automatic medical imaging diagnosis provides a non-invasive method to assist ophthalmologists in timely screening of suspected DR cases, which prevents its further deterioration. However, the state-of-the-art deep-learning-based methods generally have a large amount of model parameters, which makes large-scale clinical deployment a time-consuming task. Moreover, the severity of DR is associated with lesions, and it is difficult for the model to focus on these regions. In this paper, we propose a novel deep-learning technique for grading DR with only image-level supervision. Specifically, we first customize the model with the help of self-knowledge distillation to achieve a trade-off between model performance and time complexity. Secondly, CAM-Attention is used to allow the network to focus on discriminative zone, e.g., microaneurysms, soft/hard exudates, etc.. Considering that directly attaching a classifier after the Side branch will disrupt the hierarchical nature of convolutional neural networks, a Mimicking Module is employed that allows the Side branch to actively mimic the main branch structure. Extensive experiments are conducted on two benchmark datasets, with an AUC of 0.965 and an accuracy of 92.9% for the Messidor dataset and 67.96% accuracy achieved for the challenging IDRID dataset, which demonstrates the superior performance of our proposed method.
Evolution of V2X Communication and Integration of Blockchain for Security Enhancements
Authors: Shrestha Rakesh , Nam Seung Yeob , Bajracharya Rojeena , Kim Shiho
Published: August 2020

With the rapid evolution in wireless communications and autonomous vehicles, intelligent and autonomous vehicles will be launched soon. Vehicle to Everything (V2X) communications provides driving safety, traffic efficiency, and road information in real-time in vehicular networks. V2X has evolved by integrating cellular 5G and New Radio (NR) access technology in V2X communications (i.e., 5G NR V2X); it can fulfill the ever-evolving vehicular application, communication, and service demands of connected vehicles, such as ultra-low latency, ultra-high bandwidth, ultra-high reliability, and security. However, with the increasing number of intelligent and autonomous vehicles and their safety requirements, there is a backlash in deployment and management because of scalability, poor security and less flexibility. Multi-access Edge Computing (MEC) plays a significant role in bringing cloud services closer to vehicular nodes, which reduces the scalability and flexibility issues. In addition, blockchain has evolved as an effective technology enabler to solve several security, privacy, and networking issues faced by the current 5G-based MEC systems in vehicular networks. Blockchain can be integrated as a strong security mechanism for securing and managing 5G V2X along with MEC. In this survey, we discuss, in detail, state-of-the-art V2X, its evolution based on cellular 5G technology and non-cellular 802.11bd. We investigate the integration of blockchain in 5G-based MEC vehicular networks for security, privacy protection, and content caching. We present the issues and challenges in existing edge computing and 5G V2X and, then, we shed some light on future research directions in these integrated and emerging technologies.
Analysis of the Multi-Steps Package (MSP) for Series-Connected SiC-MOSFETs
Authors: Alves Luciano F. S. , Lefranc Pierre , Jeannin Pierre-Olivier , Sarrazin Benoit , Crebier Jean-Christophe
Published: August 2020

In this paper, a multi-step packaging (MSP) concept for series-connected SiC-MOSFETs is analyzed. The parasitic capacitance generated by the dielectric isolation of each device in the stack has a significant impact on the dynamic behavior of SiC devices, which impacts the voltage-sharing performances. The study performed in this work reveals that the parasitic capacitance network introduced by the classical planar packaging unbalances the voltage across the series-connected SiC-MOSFETs. Therefore, a new drain-source parasitic capacitance network configuration provided by the MSP is proposed in order to improve the voltage balancing across the series-connected devices. The concept is introduced and analyzed thanks to equivalent models and time domain simulations. To verify the analysis, the voltage sharing between four series-connected 1.2 kV SiC MOSFETs is tested in a double pulse test setup. The experimental results confirm that the MSP has a better performance than the classical one in terms of voltage sharing. Furthermore, the proposed investigation shows that the MSP increases the middle point dv/dt of the switching cell. Sensitive analysis and thermal management considerations are also discussed in order to clarify the MSP limitations and indicate the ways to optimize the MSP from a thermal point of view.
Performance Comparison of PD Data Acquisition Techniques for Condition Monitoring of Medium Voltage Cables
Authors: Shafiq Muhammad , Kiitam Ivar , Kauhaniemi Kimmo , Taklaja Paul , Kütt Lauri , Palu Ivo
Published: August 2020

Already installed cables are aging and the cable network is growing rapidly. Improved condition monitoring methods are required for greater visibility of insulation defects in the cable networks. One of the critical challenges for continuous monitoring is the large amount of partial discharge (PD) data that poses constraints on the diagnostic capabilities. This paper presents the performance comparison of two data acquisition techniques based on phase resolved partial discharge (PRPD) and pulse acquisition (PA). The major contribution of this work is to provide an in-depth understanding of these techniques considering the perspective of randomness of the PD mechanism and improvements in the reliability of diagnostics. Experimental study is performed on the medium voltage (MV) cables in the laboratory environment. It has been observed that PRPD based acquisition not only requires a significantly larger amount of data but is also susceptible to losing the important information especially when multiple PD sources are being investigated. On the other hand, the PA technique presents improved performance for PD diagnosis. Furthermore, the use of the PA technique enables the efficient practical implementation of the continuous PD monitoring by reducing the amount of data that is acquired by extracting useful signals and discarding the silent data intervals.
Development of Home Energy Management Scheme for a Smart Grid Community
Authors: Rashid Md Mamun Ur , Granelli Fabrizio , Hossain Md. Alamgir , Alam Md. Shafiul , Al-Ismail Fahad Saleh , Karmaker Ashish Kumar , Rahaman Md. Mijanur
Published: August 2020

The steady increase in energy demand for residential consumers requires an efficient energy management scheme. Utility organizations encourage household applicants to engage in residential energy management (REM) system. The utility’s primary goal is to reduce system peak load demand while consumer intends to reduce electricity bills. The benefits of REM can be enhanced with renewable energy sources (RESs), backup battery storage system (BBSS), and optimal power-sharing strategies. This paper aims to reduce energy usages and monetary cost for smart grid communities with an efficient home energy management scheme (HEMS). Normally, the residential consumer deals with numerous smart home appliances that have various operating time priorities depending on consumer preferences. In this paper, a cost-efficient power-sharing technique is developed which works based on priorities of appliances’ operating time. The home appliances are sorted on priority basis and the BBSS are charged and discharged based on the energy availability within the smart grid communities and real time energy pricing. The benefits of optimal power-sharing techniques with the RESs and BBSS are analyzed by taking three different scenarios which are simulated by C++ software package. Extensive case studies are carried out to validate the effectiveness of the proposed energy management scheme. It is demonstrated that the proposed method can save energy and reduce electricity cost up to 35% and 45% compared to the existing methods.
A Coolant Circulation Cooling System Combining Aluminum Plates and Copper Rods for Li-Ion Battery Pack
Authors: Zhang Chuanwei , Xia Zhan , Gao Huaibin , Wen Jianping , Chen Shangrui , Dang Meng , Gu Sujing , Zhang Jianing
Published: August 2020

The spontaneous combustion of electric vehicles occurs frequently, and the main reason is the thermal runaway of a lithium-ion battery. In order to prevent the heat that is produced in the use of a lithium-ion battery out of control, this study proposed a coolant circulation cooling system, that is, the heat generated by the lithium-ion battery is transferred to heat sinks through aluminum plates and copper rods, and then dissipated through the coolant. Based on a CALB-LB5F73 LiFePO4 battery pack, experiments with the coolant circulation cooling system were conducted to study the temperature rise characteristics at different ambient temperatures. The temperature of the battery pack was still close to the upper limit of permitted temperature when the ambient temperature reached 313 K. Further improvement, increasing the diameter of copper rod of the system was proposed to enhance heat dissipation and simulations with this scheme were completed. The findings show that the cooling system can clearly reduce the temperature of a lithium-ion battery pack and control the temperature within the safe temperature range.
FP-TES: A Fluidisation-Based Particle Thermal Energy Storage, Part I: Numerical Investigations and Bulk Heat Conductivity
Authors: Wünsch David , Sulzgruber Verena , Haider Markus , Walter Heimo
Published: August 2020

Renewables should become more continuously available, reliable and cost-efficient to manage the challenges caused by the energy transition. Thus, analytic and numerical investigations for the layout of a pilot plant of a concept called Fluidisation-Based Particle Thermal Energy Storage (FP-TES)—a highly flexible, short- to long-term fluidised bed regenerative heat storage utilising a pressure gradient for hot powder transport, and thus enabling minimal losses, high energy densities, compact construction and countercurrent heat exchange—are presented in this article. Such devices in decentralised set-up—being included in energy- and especially heat-intensive industries, storing latent or sensible heat or power-to-heat to minimise losses and compensate fluctuations—can help to achieve the above-stated goals. Part I of this article is focused on geometrical and fluidic design via numerical investigations utilising Computational Particle Fluid Dynamics (CPFD). In the process a controlled transient simulation method called co-simulation of FP-TES is developed forming the basis for test bench design and execution of further co-simulation. Within this process an advanced design of rotational symmetric hoppers with additional baffles in the heat exchanger (HEX) and internal pipes to stabilise the particle mass flow is developed. Moreover, a contribution bulk heat conductivity is presented to demonstrate low thermal losses and limited needs for thermal insulation by taking into account the thermal insulation of the outer layer of the hopper.
Volt-Var Curve Reactive Power Control Requirements and Risks for Feeders with Distributed Roof-Top Photovoltaic Systems
Authors: Jones C. Birk , Lave Matthew , Reno Matthew J. , Darbali-Zamora Rachid , Summers Adam , Hossain-McKenzie Shamina
Published: August 2020

The benefits and risks associated with Volt-Var Curve (VVC) control for management of voltages in electric feeders with distributed, roof-top photovoltaic (PV) can be defined using a stochastic hosting capacity analysis methodology. Although past work showed that a PV inverter’s reactive power can improve grid voltages for large PV installations, this study adds to the past research by evaluating the control method’s impact (both good and bad) when deployed throughout the feeder within small, distributed PV systems. The stochastic hosting capacity simulation effort iterated through hundreds of load and PV generation scenarios and various control types. The simulations also tested the impact of VVCs with tampered settings to understand the potential risks associated with a cyber-attack on all of the PV inverters scattered throughout a feeder. The simulation effort found that the VVC can have an insignificant role in managing the voltage when deployed in distributed roof-top PV inverters. This type of integration strategy will result in little to no harm when subjected to a successful cyber-attack that alters the VVC settings.
Profiling of Widely Targeted Metabolomics for the Identification of Secondary Metabolites in Heartwood and Sapwood of the Red-Heart Chinese Fir (Cunninghamia Lanceolata)
Authors: Cao Sen , Zhang Zijie , Sun Yuhan , Li Yun , Zheng Huiquan
Published: August 2020

The chemical composition of secondary metabolites is important for the quality control of wood products. In this study, the widely targeted metabolomics approach was used to analyze the metabolic profiles of heartwood and sapwood in the red-heart Chinese fir (Cunninghamia lanceolata), with an ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry system. A total of 224 secondary metabolites were detected in the heartwood and sapwood, and of these, flavonoids and phenolic acids accounted for 36% and 26% of the components, respectively. The main pathways appeared to be differentially activated, including those for the biosynthesis of phenylpropanoids and flavonoids. Moreover, we observed highly significant accumulation of naringenin chalcone, dihydrokaempferol, pinocembrin, hesperetin, and other important secondary metabolites in the flavonoid biosynthesis pathway. Our results provide insight into the flavonoid pathway associated with wood color formation in Chinese fir that will be useful for further breeding programs.
Emotional Competence Mediates the Relationship between Communication Problems and Reactive Externalizing Problems in Children with and without Developmental Language Disorder: A Longitudinal Study
Authors: van den Bedem Neeltje P. , Dockrell Julie E. , van Alphen Petra M. , Rieffe Carolien
Published: August 2020

Language problems are a risk factor for externalizing problems, but the developmental path remains unclear. Emotional competence may mediate the relationship, especially when externalizing problems are reactive in nature, such as in Oppositional Deviant Disorder (ODD) and reactive aggression. We examined the development of reactive and proactive externalizing problems in children with (n = 98) and without (n = 156) Developmental Language Disorder (DLD; age: 8–16 years) over 18 months. Relationships with communicative risk factors (structural, pragmatic and emotion communication) and the mediating role of emotional competence (emotion recognition and anger dysregulation) were examined. Multi-level analyses showed that increasing emotion recognition and decreasing anger dysregulation were longitudinally related to decreasing ODD symptoms in both groups, whereas anger dysregulation was related to more reactive aggression in children with DLD alone. Pragmatic and emotion communication problems were related to more reactive externalizing problems, but these relationships were mediated by emotional competence, suggesting that problems in emotional competence explain the communication problems of children with DLD. Therefore, in addition to interventions for communication skills, there is a need to address the emotional competence of children with DLD, as this decreases the risk for reactive externalizing problems.
Cost Effectiveness Analysis Using Disability-Adjusted Life Years for Cataract Surgery
Authors: Rochmah Thinni Nurul , Wulandari Anggun , Dahlui Maznah , Ernawaty  , Wulandari Ratna Dwi
Published: August 2020

Cataracts are the second most prioritized eye disease in the world. Cataracts are an expensive treatment because surgery is the only method that can treat the disease. This study aims to analyze the cost effectiveness of each operating procedure. Specifically, phacoemulsification and Small Incision Cataract Surgery (SICS) with Disability-Adjusted Life Years (DALYs) as the effectiveness indicator is used. This study is an observational analytic study with a prospective framework. The sample size is 130 patients who have undergone phacoemulsification and 25 patients who have undergone SICS. The DALY for phacoemulsification at Day-7 (D-7) is 0.3204, and at Day-21 (D-21), it is 0.3204, while the DALY for SICS at D-7 is 0.3060, and at D-21, it is 0.3158. The incremental cost effectiveness ratio (ICER) for cataract surgery at D-7 is USD $1872.49, and at D-21, it is USD $5861.71, whereas the Indonesian Gross Domestic Product (GDP) is USD $4174.90. In conclusion, the phacoemulsification technique is more cost effective than the SICS technique. The ICER value is very cost effective at D-7 post-surgery compared to at D-21 post-surgery because the ICER is less than 1 GDP per capita per DALY.
An Electromyographic Analysis of Lateral Raise Variations and Frontal Raise in Competitive Bodybuilders
Authors: Coratella Giuseppe , Tornatore Gianpaolo , Longo Stefano , Esposito Fabio , Cè Emiliano
Published: August 2020

The present study examined the muscle activation in lateral raise with humerus rotated externally (LR-external), neutrally (LR-neutral), internally (LR-internal), with flexed elbow (LR-flexed) and frontal raise during both the concentric and eccentric phase. Ten competitive bodybuilders performed the exercises. Normalized surface electromyographic root mean square (sEMG RMS) was obtained from anterior, medial, and posterior deltoid, pectoralis major, upper trapezius, and triceps brachii. During the concentric phase, anterior deltoid and posterior deltoid showed greater sEMG RMS in frontal raise (effect size (ES)-range: 1.78/9.25)) and LR-internal (ES-range: 10.79/21.34), respectively, vs. all other exercises. Medial deltoid showed greater sEMG RMS in LR-neutral than LR-external (ES: 1.47 (95% confidence-interval—CI: 0.43/2.38)), frontal raise (ES: 10.28(95% CI: 6.67/13.01)), and LR-flexed (ES: 6.41(95% CI: 4.04/8.23)). Pectoralis major showed greater sEMG RMS in frontal raise vs. all other exercises (ES-range: 17.2/29.5), while upper trapezius (ES-range: 2.66/7.18) and triceps brachii (ES-range: 0.41/3.31) showed greater sEMG RMS in LR-internal vs. all other exercises. Similar recruitment patterns were found during the eccentric phase. When humerus rotates internally, greater activation of posterior deltoid, triceps brachii, and upper trapezius occurs. Humerus external rotation increases the activation of anterior and medial deltoid. Frontal raise mainly activates anterior deltoid and pectoralis major. LR variations and frontal raise activate specifically shoulders muscles and should be proposed accordingly.
Demolition Activity and Elevated Blood Lead Levels among Children in Detroit, Michigan, 2014–2018
Authors: Bezold Carla , Bauer Samantha J. , Buckley Jessie P. , Batterman Stuart , Haroon Haifa , Fink Lauren
Published: August 2020

Older buildings in the United States often contain lead paint, and their demolition poses the risk of community lead exposure. We investigated associations between demolitions and elevated blood lead levels (EBLLs) among Detroit children aged <6 years, 2014–2018, and evaluated yearly variation given health and safety controls implemented during this time. Case-control analysis included incident EBLL cases (≥5 µg/dL) and non-EBLL controls from test results reported to the Michigan Department of Health and Human Services. Exposure was defined as the number of demolitions (0, 1, 2+) within 400 feet of the child’s residence 45 days before the blood test. We used logistic regression to calculate odds ratios (ORs) and 95% confidence intervals (CIs), and test effect modification by year. Associations between demolition and EBLL differed yearly (p = 0.07): 2+ demolitions were associated with increased odds of EBLLs in 2014 (OR: 1.75; 95% CI: (1.17, 2.55), 2016 (2.36; 1.53, 3.55) and 2017 (2.16; 1.24, 3.60), but not in 2018 (0.94; 0.41, 1.86). This pattern remained consistent in sensitivity analyses. The null association in 2018 may be related to increased health and safety controls. Maintenance of controls and monitoring are essential, along with other interventions to minimize lead exposure, especially for susceptible populations.
The Homeostatic Theory of Obesity: An Empirical Verification of the Circle of Discontent with an Assessment of Its Relationship to Restrained and Uncontrolled Eating among Children and Adolescents
Authors: Czepczor-Bernat Kamila , Brytek-Matera Anna , Matusik Paweł
Published: August 2020

The purpose of the present study was to provide an empirical verification of the Circle of Discontent with an assessment of its relationship to restrained and uncontrolled eating among children and adolescents. This study examined whether our results confirm a new hypothesized model. The total sample comprised 282 children and adolescents (148 girls and 134 boys; 141 participants with normal body weight and 141 with obesity). The mean age was 12.23 years (SD = 2.80), and the average BMI (body mass index) was 23.29 kg/m2 (SD = 6.27). The following were used: Positive and Negative Affect Scale for Children, Children’s Body Image Scale, Figure Rating Scale, Three-Factor Eating Questionnaire and Eating Disorders in Youth. The obtained values of the model fit indices proved the goodness of fit. Our findings show that obesity accompanies body dissatisfaction and uncontrolled and restrictive eating. Moreover, the higher the level of restrictive eating, the lower the level of uncontrolled eating. The relationships between body dissatisfaction, negative affect and restrictive eating, as well as that between uncontrolled eating and high-energy consumption, are significant and positive. Other relationships are non-significant. The above-mentioned relationships established in the Circle of Discontent, as well as relationships of restrained and uncontrolled eating with variables described in the circle, were confirmed. Based on our results, preventive strategies and psychological interventions can be created and may include changes in body image, eating behaviors and emotional functioning.
Biomedical Holistic Ontology for People with Rare Diseases
Authors: Subirats Laia , Conesa Jordi , Armayones Manuel
Published: August 2020

This research provides a biomedical ontology to adequately represent the information necessary to manage a person with a disease in the context of a specific patient. A bottom-up approach was used to build the ontology, best ontology practices described in the literature were followed and the minimum information to reference an external ontology term (MIREOT) methodology was used to add external terms of other ontologies when possible. Public data of rare diseases from rare associations were used to build the ontology. In addition, sentiment analysis was performed in the standardized data using the Python library Textblob. A new holistic ontology was built, which models 25 real scenarios of people with rare diseases. We conclude that a comprehensive profile of patients is needed in biomedical ontologies. The generated code is openly available, so this research is partially reproducible. Depending on the knowledge needed, several views of the ontology should be generated. Links to other ontologies should be used more often to model the knowledge more precisely and improve flexibility. The proposed holistic ontology has many benefits, such as a more standardized computation of sentiment analysis between attributes.
Anterograde Neuronal Circuit Tracers Derived from Herpes Simplex Virus 1: Development, Application, and Perspectives
Authors: Li Dong , Yang Hong , Xiong Feng , Xu Xiangmin , Zeng Wen-Bo , Zhao Fei , Luo Min-Hua
Published: August 2020

Herpes simplex virus type 1 (HSV-1) has great potential to be applied as a viral tool for gene delivery or oncolysis. The broad infection tropism of HSV-1 makes it a suitable tool for targeting many different cell types, and its 150 kb double-stranded DNA genome provides great capacity for exogenous genes. Moreover, the features of neuron infection and neuron-to-neuron spread also offer special value to neuroscience. HSV-1 strain H129, with its predominant anterograde transneuronal transmission, represents one of the most promising anterograde neuronal circuit tracers to map output neuronal pathways. Decades of development have greatly expanded the H129-derived anterograde tracing toolbox, including polysynaptic and monosynaptic tracers with various fluorescent protein labeling. These tracers have been applied to neuroanatomical studies, and have contributed to revealing multiple important neuronal circuits. However, current H129-derived tracers retain intrinsic drawbacks that limit their broad application, such as yet-to-be improved labeling intensity, potential nonspecific retrograde labeling, and high toxicity. The biological complexity of HSV-1 and its insufficiently characterized virological properties have caused difficulties in its improvement and optimization as a viral tool. In this review, we focus on the current H129-derived viral tracers and highlight strategies in which future technological development can advance its use as a tool.
Role of Adipose Tissue-Derived Autotaxin, Lysophosphatidate Signaling, and Inflammation in the Progression and Treatment of Breast Cancer
Authors: Brindley David N. , Tang Xiaoyun , Meng Guanmin , Benesch Matthew G. K.
Published: August 2020

Autotaxin (ATX) is a secreted enzyme that produces lysophosphatidate (LPA), which signals through six G-protein coupled receptors, promoting tumor growth, metastasis, and survival from chemotherapy and radiotherapy. Many cancer cells produce ATX, but breast cancer cells express little ATX. In breast tumors, ATX is produced by tumor-associated stroma. Breast tumors are also surrounded by adipose tissue, which is a major bodily source of ATX. In mice, a high-fat diet increases adipocyte ATX production. ATX production in obesity is also increased because of low-level inflammation in the expanded adipose tissue. This increased ATX secretion and consequent LPA signaling is associated with decreased adiponectin production, which results in adverse metabolic profiles and glucose homeostasis. Increased ATX production by inflamed adipose tissue may explain the obesity-breast cancer association. Breast tumors produce inflammatory mediators that stimulate ATX transcription in tumor-adjacent adipose tissue. This drives a feedforward inflammatory cycle since increased LPA signaling increases production of more inflammatory mediators and cyclooxygenase-2. Inhibiting ATX activity, which has implications in breast cancer adjuvant treatments, attenuates this cycle. Targeting ATX activity and LPA signaling may potentially increase chemotherapy and radiotherapy efficacy, and decrease radiation-induced fibrosis morbidity independently of breast cancer type because most ATX is not derived from breast cancer cells.
Histone H1 Post-Translational Modifications: Update and Future Perspectives
Authors: Andrés Marta , García-Gomis Daniel , Ponte Inma , Suau Pedro , Roque Alicia
Published: August 2020

Histone H1 is the most variable histone and its role at the epigenetic level is less characterized than that of core histones. In vertebrates, H1 is a multigene family, which can encode up to 11 subtypes. The H1 subtype composition is different among cell types during the cell cycle and differentiation. Mass spectrometry-based proteomics has added a new layer of complexity with the identification of a large number of post-translational modifications (PTMs) in H1. In this review, we summarize histone H1 PTMs from lower eukaryotes to humans, with a particular focus on mammalian PTMs. Special emphasis is made on PTMs, whose molecular function has been described. Post-translational modifications in H1 have been associated with the regulation of chromatin structure during the cell cycle as well as transcriptional activation, DNA damage response, and cellular differentiation. Additionally, PTMs in histone H1 that have been linked to diseases such as cancer, autoimmune disorders, and viral infection are examined. Future perspectives and challenges in the profiling of histone H1 PTMs are also discussed.
High Numbers and Densities of PD1+ T-Follicular Helper Cells in Triple-Negative Breast Cancer Draining Lymph Nodes Are Associated with Lower Survival
Authors: Bronsert Peter , von Schoenfeld Anna , Villacorta Hidalgo Jose , Kraft Stefan , Pfeiffer Jens , Erbes Thalia , Werner Martin , Seidl Maximilian
Published: August 2020

Breast cancer tumor draining lymph nodes (TDLNs) display distinct morphologic changes depending on the breast cancer subtype. For triple-negative breast cancers (TNBC), draining LNs display a higher amount of secondary lymphoid follicles, which can be regarded as a surrogate marker for an activated humoral immune response. In the present study, we focus on PD1+ T-follicular helper cells (Tfh) in TDLNs of TNBC, since PD1+ Tfh are drivers of the germinal center (GC) reaction. We quantified PD1+ Tfh in 22 sentinel LNs with 853 GCs and interfollicular areas from 19 patients with TNBC by morphometry from digitalized immunostained tissue sections. Overall survival was significantly worse for patients with a higher number and area density of PD1+ Tfh within GCs of TDLNs. Further, we performed T-cell receptor gamma chain (TRG) analysis from microdissected tissue in the primary tumor and TDLNs. Eleven patients showed the same TRG clones in the tumor and the LN. Five patients shared the same TRG clones in the tumor and the GCs. In two patients, those clones were highly enriched inside the GCs. Enrichment of identical TRG clones at the tumor site vs. the TDLN was associated with improved overall survival. TDLNs are important relays of cancer immunity and enable surrogate approaches to predict the outcome of TNBC itself.
Biomaterials Loaded with Growth Factors/Cytokines and Stem Cells for Cardiac Tissue Regeneration
Authors: Smagul Saltanat , Kim Yevgeniy , Smagulova Aiganym , Raziyeva Kamila , Nurkesh Ayan , Saparov Arman
Published: August 2020

Myocardial infarction causes cardiac tissue damage and the release of damage-associated molecular patterns leads to activation of the immune system, production of inflammatory mediators, and migration of various cells to the site of infarction. This complex response further aggravates tissue damage by generating oxidative stress, but it eventually heals the infarction site with the formation of fibrotic tissue and left ventricle remodeling. However, the limited self-renewal capability of cardiomyocytes cannot support sufficient cardiac tissue regeneration after extensive myocardial injury, thus, leading to an irreversible decline in heart function. Approaches to improve cardiac tissue regeneration include transplantation of stem cells and delivery of inflammation modulatory and wound healing factors. Nevertheless, the harsh environment at the site of infarction, which consists of, but is not limited to, oxidative stress, hypoxia, and deficiency of nutrients, is detrimental to stem cell survival and the bioactivity of the delivered factors. The use of biomaterials represents a unique and innovative approach for protecting the loaded factors from degradation, decreasing side effects by reducing the used dosage, and increasing the retention and survival rate of the loaded cells. Biomaterials with loaded stem cells and immunomodulating and tissue-regenerating factors can be used to ameliorate inflammation, improve angiogenesis, reduce fibrosis, and generate functional cardiac tissue. In this review, we discuss recent findings in the utilization of biomaterials to enhance cytokine/growth factor and stem cell therapy for cardiac tissue regeneration in small animals with myocardial infarction.
The Plasticity of Root Systems in Response to External Phosphate
Authors: Huang Guoqiang , Zhang Dabing
Published: August 2020

Phosphate is an essential macro-element for plant growth accumulated in the topsoil. The improvement of phosphate uptake efficiency via manually manipulating root system architecture is of vital agronomic importance. This review discusses the molecular mechanisms of root patterning in response to external phosphate availability, which could be applied on the alleviation of phosphate-starvation stress. During the long time evolution, plants have formed sophisticated mechanisms to adapt to environmental phosphate conditions. In terms of root systems, plants would adjust their root system architecture via the regulation of the length of primary root, the length/density of lateral root and root hair and crown root growth angle to cope with different phosphate conditions. Finally, plants develop shallow or deep root system in low or high phosphate conditions, respectively. The plasticity of root system architecture responds to the local phosphate concentrations and this response was regulated by actin filaments, post-translational modification and phytohormones such as auxin, ethylene and cytokinin. This review summarizes the recent progress of adaptive response to external phosphate with focus on integrated physiological, cellular and molecular signaling transduction in rice and Arabidopsis.
Sex-Specific Wolbachia Infection Patterns in Populations of Polygraphus proximus Blandford (Coleoptera; Curculionidae: Scolytinae)
Authors: Bykov Roman , Kerchev Ivan , Demenkova Marya , Ryabinin Artem , Ilinsky Yury
Published: August 2020

Wolbachia symbionts are maternally inherited bacteria that are widely distributed among Arthropoda hosts. Wolbachia influence their host biology in diverse ways. They may induce reproductive abnormalities, protect hosts against pathogens and parasites, or benefit hosts through metabolic provisioning. The progeny of an infected female are ordinarily infected with Wolbachia; however, Wolbachia have no future in male host progeny because they cannot transmit the symbiont to the next generation. Here, we analyze native and invasive populations of the four-eyed fir bark beetle (Polygraphus proximus) for Wolbachia prevalence and symbiont genetic diversity. This species is a dangerous pest of Siberian fir (Abies sibirica) forests. The native range of P. proximus includes the territories of the Russian Far East, Japan, Korea, and Northeast China, whereas its invasive range includes West Siberia, with further expansion westward. Surprisingly, we revealed a difference in the patterns of Wolbachia prevalence for males and females. Infection rate and Wolbachia titers were higher in females than in males. ST-533, the only haplotype of Wolbachia supergroup B, was associated with a minimum of three out of the five described mitochondrial haplotypes.
Preliminary Mechanical Analysis of Rubber-Cement Composites Suitable for Additive Process Construction
Authors: Sambucci Matteo , Marini Danilo , Sibai Abbas , Valente Marco
Published: August 2020

Additive manufacturing for cementitious materials represents the most attractive frontier in the modern context of Construction 4.0. In addition to the technological progress of printing systems, the development of functional and low environmental impact printable mixtures is one of the current challenges of digital fabrication in building and architectural fields. This paper proposes a preliminary physical-mechanical analysis on environmentally friendly mortars, compatible with the extrusion-based printing process, made up of recycling rubber aggregates deriving from end-of-life tires. In this study, two groups of rubber particle samples (0–1 mm rubber powder and 2–4 mm rubber granules) were used to partially/totally replace the mineral fraction of the reference printable mixture. Four tire rubber powder-granules proportions were investigated and control mortar (100% sand) was also prepared to compare its properties with those of the rubber-cement samples in terms of printability properties, mechanical strength, ductility, and structural isotropy. Based on the experimental results, the rubber aggregates increase the mixture fluidity, promoting better inter-layer adhesion than the neat mix. This leads to greater mechanical isotropy. As already investigated in other research works on Rubber-Concrete technology, the addition of rubber particles increases the ductility of the material but reduces its mechanical strength. However, by correctly balancing the fine and coarse rubber fraction, promising physical-mechanical performances were demonstrated.