bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of the Monophyletic Lineage of Avian Influenza H5N1 Which Circulated in Venezuelan Birds During the 2022–2023 Outbreak
Authors: Loureiro Carmen Luisa , Bonetti Valeria , Jaspe Rossana C. , Sulbaran Yoneira , Alcazar Wilmer , Hernández Carlos , Rodríguez Nardraka , Rangel Hector R. , Zambrano Jose Luis , Pujol Flor H.
Published: December 2024

Avian influenza subtype H5N1 has caused outbreaks worldwide since 1996, with the emergence of the Guandong lineage in China. The current clade 2.3.4.4b has evolved from this lineage, with increased virulence and mass mortality events in birds and mammals. The objective of this study was the analysis of 17 viral genomes of H5N1 avian influenza isolated in Venezuela during the 2022–2023 outbreak. The eight viral genomic segments were amplified using universal primers and sequenced via next-generation sequencing. The sequences were analyzed to confirm the H5 hemagglutinin clade, identify possible genetic reassortments, and perform a phylogenetic and docking analysis of the viral isolates. The viruses found in Venezuela belonged, as expected, to clade 2.3.4.4b and formed a monophyletic clade with North American influenza viruses, with no evidence of further reassortment. The introduction of the virus in South America is associated with bird migration through the Atlantic (Venezuela), Atlantic/Mississippi (Choco, Colombia), and Pacific migratory flyways, with the emergence of several viral lineages. Several mutations were found in all segments of the genome, although none of the key mutations was involved in mammalian adaptation. Moreover, in silico structural analysis suggests, as expected, that the viral hemagglutinin maintained a predilection for avian α2,3-linked sialic acid. The unprecedented pathogenic outbreak of avian influenza disease in South America was associated with the circulation of three different lineages, which maintain a lower affinity for the mammalian receptor.
Multi-Resonant Full-Solar-Spectrum Perfect Metamaterial Absorber
Authors: Shen Zhe , Ni Junfan
Published: December 2024

Currently, perfect absorption properties of metamaterials have attracted widespread interest in the area of solar energy. Ultra-broadband absorption, incidence angle insensitivity, and polarization independence are key performance indicators in the design of the absorbers. In this work, we proposed a metamaterial absorber based on the absorption mechanism with multiple resonances, including propagation surface plasmon resonance (PSPR), localized surface plasmon resonance (LSPR), electric dipole resonance (EDR), and magnetic dipole resonance (MDR). The absorber, consisting of composite nanocylinders and a microcavity, can perform solar energy full-spectrum absorption. The proposed absorber obtained high absorption (>95%) from 272 nm to 2742 nm at normal incidence. The weighted absorption rate of the absorber at air mass 1.5 direct in the wavelength range of 280 nm to 3000 nm exceeds 98.5%. The ultra-broadband perfect absorption can be ascribed to the interaction of those resonances. The photothermal conversion efficiency of the absorber reaches 85.3% at 375 K. By analyzing the influence of the structural parameters on the absorption efficiency, the absorber exhibits excellent fault tolerance. In addition, the designed absorber is insensitive to polarization and variation in ambient refractive index and has an absorption rate of more than 80% at the incident angle of 50°. Our proposed absorber has great application potential in solar energy collection, photothermal conversion, and other related areas.
Non-Destructive Testing of Concrete Materials from Piers: Evaluating Durability Through a Case Study
Authors: Lopez-Miguel Abraham , Cabello-Mendez Jose A. , Moreno-Valdes Alejandro , Perez-Quiroz Jose T. , Machorro-Lopez Jose M.
Published: December 2024

Concrete is currently the most used construction material, mainly due to its mechanical strength, chemical stability, and low cost. This material is affected by wear processes caused by the environment, which lead to a reduction in the useful life of the infrastructure in the long term. These wear processes can cause cracks, corrosion of reinforcing steel, loss of load capacity, and loss of concrete section, among other problems. Considering the above, it is necessary to carry out durability studies on concrete to determine the integrity conditions in which the infrastructure is found, the reasons for its deterioration, the environmental factors that affect it, and its useful life under these conditions, and develop restoration or protection plans. Generally, the durability studies include non-destructive testing such as ultrasonic pulse velocity, electrical resistivity, porosity measurement, and capillary absorption rate. These techniques make it possible to characterize the concrete and obtain information such as the total volume of pores, susceptibility to corrosion of the reinforcing steel, decrease in mechanical resistance, cracks, presence of humidity, and aggressive ions inside the concrete. In this work, two durability studies are presented with non-destructive tests carried out on active piers that are 20 and 40 years old. These are located in coastal areas in southern Mexico on the Gulf of Mexico side, with 80% average annual relative humidity, temperatures above 33 °C on average, high concentrations of salts, load handling, vibrations, flora and fauna typical of the marine ecosystem, etc. The results obtained reveal important information about the current state of the piers and the damage caused by the environment over time. This information allowed us to make decisions on preventive actions and develop appropriate and specific restoration projects for each pier.
Impact of Mast Cell Activation on Neurodegeneration: A Potential Role for Gut–Brain Axis and Helicobacter pylori Infection
Authors: Boziki Marina , Theotokis Paschalis , Kesidou Evangelia , Nella Maria , Bakirtzis Christos , Karafoulidou Eleni , Tzitiridou-Chatzopoulou Maria , Doulberis Michael , Kazakos Evangelos , Deretzi Georgia , Grigoriadis Nikolaos , Kountouras Jannis
Published: December 2024

Background: The innate immune response aims to prevent pathogens from entering the organism and/or to facilitate pathogen clearance. Innate immune cells, such as macrophages, mast cells (MCs), natural killer cells and neutrophils, bear pattern recognition receptors and are thus able to recognize common molecular patterns, such as pathogen-associated molecular patterns (PAMPs), and damage-associated molecular patterns (DAMPs), the later occurring in the context of neuroinflammation. An inflammatory component in the pathology of otherwise “primary cerebrovascular and neurodegenerative” disease has recently been recognized and targeted as a means of therapeutic intervention. Activated MCs are multifunctional effector cells generated from hematopoietic stem cells that, together with dendritic cells, represent first-line immune defense mechanisms against pathogens and/or tissue destruction. Methods: This review aims to summarize evidence of MC implication in the pathogenesis of neurodegenerative diseases, namely, Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, Huntington’s disease, and multiple sclerosis. Results: In view of recent evidence that the gut–brain axis may be implicated in the pathogenesis of neurodegenerative diseases and the characterization of the neuroinflammatory component in the pathology of these diseases, this review also focuses on MCs as potential mediators in the gut–brain axis bi-directional communication and the possible role of Helicobacter pylori, a gastric pathogen known to alter the gut–brain axis homeostasis towards local and systemic pro-inflammatory responses. Conclusion: As MCs and Helicobacter pylori infection may offer targets of intervention with potential therapeutic implications for neurodegenerative disease, more clinical and translational evidence is needed to elucidate this field.
Deleterious Effects of Yoyo Dieting and Resistant Starch on Gastrointestinal Morphology
Authors: Phuong-Nguyen Kate , Mahmood Malik , Rivera Leni
Published: December 2024

Background: Obesity is associated with structural deterioration in the gut. Yoyo dieting, which refers to repeated phases of dieting and non-dieting periods leading to cyclic weight loss and regain, is a common occurrence in individuals with obesity. However, there is limited evidence on how gut structures are affected in yoyo dieting. There is good evidence suggesting that increased intake of resistant starch (RS) may be beneficial in promoting structural improvements in the gut. This investigation aimed to explore the effect of yoyo dieting on gastrointestinal structure and whether RS has beneficial effects in improving obesity-related gastrointestinal damage. Method: In this study, male and female C57BL/6 mice were assigned to six different diets for 20 weeks: (1) control diet, (2) high fat diet (HF), (3) yoyo diet (alternating HF and control diets every 5 weeks), (4) control diet with RS, (5) HF with RS, and (6) yoyo diet with RS. Distal colon was collected for epithelial barrier integrity measurement. The small and large intestines were collected for histological assessment. Results: After 20 weeks, yoyo dieting resulted in increased colonic inflammation and exacerbated mucosal damage in comparison with continuous HF diet feeding. RS supplemented in HF and yoyo diets reduced mucosal damage in comparison to diets without RS. However, RS supplementation in a control diet significantly increased inflammation, crypt length, and goblet cell density. There were no significant differences in epithelial change and epithelial barrier integrity across diet groups. Conclusions: This study suggests that yoyo dieting worsens gut damage, and incorporating high levels of RS may be detrimental in the absence of dietary challenge.
The Long-Lasting Effect of Multidisciplinary Interventions for Emotional and Social Loneliness in Older Community-Dwelling Individuals: A Systematic Review
Authors: Zaharia Georgiana , Ibáñez-del Valle Vanessa , Cauli Omar , Corchón Silvia
Published: December 2024

Background: Loneliness can occur at any age, but it is more prevalent among older adults due to the associated risk factors. Various interventions exist to improve this situation, but little is known about their long-term effects. Our aims were to determine if these interventions have long-lasting effects and for how long they can be sustained. Additionally, we aimed to analyze if the interventions carried out by volunteers affected the outcomes regarding loneliness and psychological impact. Methods: A systematic review was performed by searching the literature in the MEDLINE PubMed, SCOPUS, Web of Science, PsycINFO, and Web of Science databases for interventions focused on the lonely population. The inclusion criteria for this review were the assessment of loneliness using a validated tool, and loneliness being the primary or secondary outcome. The CASPe checklist was used to assess the risk of bias in the selected studies, and the PRISMA-ScR recommendations were followed to present and synthesize the results. Results: Thirty articles were included. The interventions identified were classified into five categories: psychosocial, technological, health promotion, physical exercise, and multicomponent interventions. Loneliness improved in 24 studies during the post-intervention analysis. Social connectivity and depressive symptoms also improved in most interventions. Long-term follow-ups were conducted with positive results in a total of 16 interventions. Depressive symptoms and social connectivity were also improved. Eight of the interventions were carried out by volunteers and showed good results regarding loneliness. Conclusions: The results obtained in this work suggested that multidisciplinary interventions can reduce loneliness, but more controlled clinical studies are needed.
Providing Physical Health Checks for People with Severe Mental Illness in Primary Care in England: An Evaluation of a Locally Enhanced Service
Authors: Hardy Sheila
Published: December 2024

Background/Objectives: People with a severe mental illness die much earlier than the rest of the population from a preventable physical illness. Annual health checks are a way of assessing the person to then offer the appropriate interventions. Integrated Care Northampton, England used the long-term plan baseline funding allocated to them from the government department that commissions primary care services, to implement a local enhanced service. Their aim was to provide a person-centred physical health check that people with severe mental illness feel comfortable, confident, and able to engage with. Methods: Wellbeing Organisation Research Training Hub Northampton were commissioned by Integrated Care Northampton to provide training, support, and evaluate the locally enhanced service. Training was provided by training trainers who then offered one-to-one support to those delivering health checks in practice. Providers of the health checks could also access individual support from Wellbeing Organisation Research Training Hub staff. Patient data were collected via a template that is part of usual practice. Questionnaires were used to evaluate the education of staff, the delivery of health checks, and the impact on people with severe mental illness. Results: Training was well received but most of the trainers did not continue in their role. The project was successful in highlighting the physical health needs of people with severe mental illness and monitoring in primary care increased. Though methods were put in place to evaluate the delivery of health checks and their impact on people with severe mental illness, these were not utilised by the service. Conclusions: This paper emphasises how difficult it is to implement a new service and evaluate it successfully. Future projects should prioritise measuring the quality of the service.
Prevalence of Dental Caries Among 6–11-Year-Olds in Nigeria: A Systematic Review and Meta-Analysis
Authors: Ehizele Adebola Oluyemisi , Adebayo Ezekiel Taiwo , Adewole Ifeoluwa Eunice , Abodunrin Olunike Rebecca , Akinsolu Folahanmi Tomiwa , Nwaokorie Francisca , Alade Omolola Titilayo , Salako Abideen Olurotimi , Lusher Joanne , Ezechi Oliver Chukwujekwe , Foláyan Morẹ́nikẹ́ Oluwátóyìn
Published: December 2024

Objective: This systematic review and meta-analysis aimed to assess the prevalence of dental caries among 6–11-year-old children in Nigeria and its distribution by sex, socioeconomic status, and geopolitical zones. Method: A systematic search was conducted in MEDLINE (PubMed), Google Scholar, and Cochrane Library (CENTRAL) to identify relevant studies published between 2012 and 2022. Cohort, cross-sectional, and observational studies reporting dental caries prevalence data among 6–11-year-olds in Nigeria were included. Using the random effects model, prevalence was analysed based on sex, socioeconomic status, and the country’s six geopolitical zones. The primary outcome was the pooled prevalence of dental caries. Meta-analysis was performed using the random effects model, and study quality was assessed using the Joanna Briggs Institute Critical Appraisal Checklist. The systematic review was registered with PROSPERO (CRD42023396948). Results: Data were pooled from 8 of 1237 screened articles. The pooled prevalence of dental caries was 1.5% (95% CI: −1.2–4.1). Prevalence was higher among males (1.5%, 95% CI: −1.4–4.3) compared to females (1.4%, 95% CI: −1.6–4.4). It was 0.1% (95% CI: −1.0–1.1) among those with high socioeconomic status, 0.2% (95% CI: −1.6 to 1.9) among those with middle socioeconomic status, and 0.6% (95% CI: −2.8 to 3.9) among those with low socioeconomic status. The prevalence also ranged from 1.1% (95% CI: −1.8 to 4.1) in southwest, 1.1% (95% CI: −5.4 to 7.6) in south-south, 2.3% (95% CI: −7.1 to 11.6) in southeast, and 2.3% (95% CI: −7.1 to 11.7) in northwest. Conclusions: Caries intervention among adolescents in Nigeria may need to be tailored to address the observed sex, socioeconomic status, and geopolitical zone disparities.
Antimicrobial Susceptibility Trends in E. coli Causing Pediatric Urinary Tract Infections in the United States
Authors: Mahajan Simren , Kanwar Neena , Morgan Gina M. , Mendes Rodrigo E. , Lee Brian R. , Banerjee Dithi , Selvarangan Rangaraj
Published: December 2024

Urinary tract infections (UTIs) are among the most common pediatric infections. This study evaluated the antimicrobial susceptibility patterns of 3511 uropathogenic E. coli (UPEC) isolated from pediatric patients in the United States from 2014 to 2023. The database from the SENTRY antimicrobial surveillance program from 89 medical centers was utilized as a data source. The antimicrobial susceptibility was tested using the microbroth dilution technique against 24 antimicrobial agents. MICs were determined using the CLSI/EUCAST/FDA breakpoint criteria. All the antimicrobials reported susceptibility rates above 80% except for tetracycline (76.2%), trimethoprim–sulfamethoxazole (69.7%), and ampicillin–sulbactam (55.7%). During the study period, the susceptibility rates remained stable for most antimicrobial agents. However, significant differences were observed among age, gender, and U.S. census regions, with the Middle Atlantic showing the lowest and the Mountain region the highest susceptibility rates, for most antimicrobials. The incidence of ESBL UPEC increased from 7.1% to 10.8% between 2014 and 2023, while the prevalence of the MDR phenotype remained relatively stable. The prevalence of both ESBL and MDR phenotypes was highest among infants and young children (0–24 months), with the highest resistance rates from the Pacific region. Knowledge of the landscape of antibiotic resistance in pediatric UPEC will help healthcare providers to better tailor empiric treatment regimens for most UTI infections.
Spatiotemporal Dynamics of SARS-CoV-2 Variants During the First Year of the Pandemic Highlight the Earlier Emergence of the Zeta Variant of Interest in Brazil
Authors: Moraes Marília Mazzi , Campos Guilherme Rodrigues Fernandes , Banho Cecília Artico , Versiani Alice Freitas , Lopes dos Santos Thayza Maria Izabel , Parra Maisa Carla Pereira , Lobl Edoardo , Galvão Tayna Manfrin , Vasilakis Nikos , Nogueira Maurício Lacerda
Published: December 2024

During the COVID-19 pandemic, SARS-CoV-2 caused an alarming number of cases and deaths worldwide. Brazil was severely affected from late 2020 onward, especially after the emergence of variants of concern (VOCs) and variants of interest (VOIs). Although much is known about the dynamics and evolution of SARS-CoV-2 VOIs and VOCs in the country, information is still lacking on how the cocirculation of several SARS-CoV-2 lineages, along with the lack of vaccination and low adherence to social isolation measures, shaped the first year of the COVID-19 pandemic in Brazil. We used a combination of genomic and epidemiological data to understand the transmission dynamics of SARS-CoV-2 variants from March to November 2020 within a medium-sized city in São Paulo state. By generating 627 SARS-CoV-2 whole genomes, we were able to identify 10 different SARS-CoV-2 lineages that were cocirculating in the municipality. Although many introduction events have been identified, B.1.1.28 and B.1.1.33 variants were the most frequent during the sampling period. We also detected the presence of the Zeta and N.9 variants earlier than had previously been reported in Brazil. These findings reinforce the need for active genomic surveillance to detect new viral introductions that may impact health systems during public health emergencies.
Equivocating and Deliberating on the Probability of COVID-19 Infection Serving as a Risk Factor for Lung Cancer and Common Molecular Pathways Serving as a Link
Authors: Amara Abdelbasset , Trabelsi Saoussen , Hai Abdul , Zaidi Syeda Huma H. , Siddiqui Farah , Alsaeed Sami
Published: December 2024

The COVID-19 infection caused by SARS-CoV-2 in late 2019 posed unprecedented global health challenges of massive proportions. The persistent effects of COVID-19 have become a subject of significant concern amongst the medical and scientific community. This article aims to explore the probability of a link between the COVID-19 infection and the risk of lung cancer development. First, this article reports that SARS-CoV-2 induces severe inflammatory response and cellular stress, potentially leading to tumorigenesis through common pathways between SARS-CoV-2 infection and cancer. These pathways include the JAK/STAT3 pathway which is activated after the initiation of cytokine storm following SARS-CoV-2 infection. This pathway is involved in cellular proliferation, differentiation, and immune homeostasis. The JAK/STAT3 pathway is also hyperactivated in lung cancer which serves as a link thereof. It predisposes patients to lung cancer through myriad molecular mechanisms such as DNA damage, genomic instability, and cell cycle dysregulation. Another probable pathway to tumorigenesis is based on the possibility of an oncogenic nature of SARS-CoV-2 through hijacking the p53 protein, leading to cell oxidative stress and interfering with the DNA repair mechanisms. Finally, this article highlights the overexpression of the SLC22A18 gene in lung cancer. This gene can be overexpressed by the ZEB1 transcription factor, which was found to be highly expressed during COVID-19 infection.
Rat Models in Post-Traumatic Stress Disorder Research: Strengths, Limitations, and Implications for Translational Studies
Authors: Sarapultsev Alexey , Komelkova Maria , Lookin Oleg , Khatsko Sergey , Gusev Evgenii , Trofimov Alexander , Tokay Tursonjan , Hu Desheng
Published: December 2024

Post-Traumatic Stress Disorder (PTSD) is a multifaceted psychiatric disorder triggered by traumatic events, leading to prolonged psychological distress and varied symptoms. Rat models have been extensively used to explore the biological, behavioral, and neurochemical underpinnings of PTSD. This review critically examines the strengths and limitations of commonly used rat models, such as single prolonged stress (SPS), stress–re-stress (S-R), and predator-based paradigms, in replicating human PTSD pathology. While these models provide valuable insights into neuroendocrine responses, genetic predispositions, and potential therapeutic targets, they face challenges in capturing the full complexity of PTSD, particularly in terms of ethological relevance and translational validity. We assess the degree to which these models mimic the neurobiological and behavioral aspects of human PTSD, highlighting areas where they succeed and where they fall short. This review also discusses future directions in refining these models to improve their utility for translational research, aiming to bridge the gap between preclinical findings and clinical applications.
Simulator of the Human Intestinal Microbial Ecosystem (SHIME®): Current Developments, Applications, and Future Prospects
Authors: Zhu Wei , Zhang Xiaoyong , Wang Dong , Yao Qinghua , Ma Guang-Lei , Fan Xiaohui
Published: December 2024

The human gastrointestinal microbiota plays a vital role in maintaining host health and preventing diseases, prompting the creation of simulators to replicate this intricate system. The Simulator of the Human Intestinal Microbial Ecosystem (SHIME®), a multicompartment dynamic simulator, has emerged as a pivotal in vitro model for studying the interactions and interferences within the human gut microbiota. The continuous and real-time monitoring hallmarks, along with the programmatically flexible setup, bestow SHIME® with the ability to mimic the entire human intestinal ecosystem with high dynamics and stability, allowing the evaluation of various treatments on the bowel microbiota in a controlled environment. This review outlines recent developments in SHIME® systems, including the M-SHIME®, Twin-SHIME®, Triple-SHIME®, and Toddle SHIME® models, highlighting their applications in the fields of food and nutritional science, drug development, gut health research, and traditional Chinese medicine. Additionally, the prospect of SHIME® integrating with other advanced technologies is also discussed. The findings underscore the versatility of SHIME® technology, demonstrating its significant contributions to current gut ecosystem research and its potential for future innovation in microbiome-related fields.
Buccal Absorption of Biopharmaceutics Classification System III Drugs: Formulation Approaches and Mechanistic Insights
Authors: Sabra Rayan , Kirby Daniel , Chouk Vikram , Malgorzata Kleta , Mohammed Afzal R.
Published: December 2024

Buccal drug delivery emerges as a promising strategy to enhance the absorption of drugs classified under the Biopharmaceutics Classification System (BCS) Class III, characterized by high solubility and low permeability. However, addressing the absorption challenges of BCS Class III drugs necessitates innovative formulation strategies. This review delves into optimizing buccal drug delivery for BCS III drugs, focusing on various formulation approaches to improve absorption. Strategies such as permeation enhancers, mucoadhesive polymers, pH modifiers, ion pairing, and prodrugs are systematically explored for their potential to overcome challenges associated with BCS Class III drugs. The mechanistic insight into how these strategies influence drug absorption is discussed, providing a detailed understanding of their applicability. Furthermore, the review advocates for integrating conventional buccal dosage forms with these formulation approaches as a potential strategy to enhance absorption. By emphasizing bioavailability enhancement, this review contributes to a holistic understanding of optimizing buccal absorption for BCS Class III drugs, presenting a unified approach to overcome inherent limitations in their delivery.
Liposomal Formulation of an Organogold Complex Enhancing Its Activity as Antimelanoma Agent—In Vitro and In Vivo Studies
Authors: Pinho Jacinta O. , Coelho Mariana , Pimpão Catarina , Konwar Jahnobi , Godinho-Santos Ana , Noiva Rute M. , Thomas Sophie R. , Casini Angela , Soveral Graça , Gaspar Maria Manuela
Published: December 2024

Background/Objectives: The therapeutic management of melanoma, the most aggressive form of skin cancer, remains challenging. In the search for more effective therapeutic options, metal-based complexes are being investigated for their anticancer properties. Cisplatin was the first clinically approved platinum-based drug and, based on its success, other metals (e.g., gold) are being used to design novel compounds. Methods: the antimelanoma potential of a new organometallic cyclometalated Au(III) complex [[Au(CNOxN)Cl2] (CNOxN = 2-(phenyl-(2-pyridinylmethylene)aminoxy acetic acid))] (ST004) was evaluated in vitro and in vivo. Furthermore, the gold-based complex was incorporated in liposomes to overcome solubility and stability problems, to promote accumulation at melanoma sites and to maximize the therapeutic effect while controlling its reactivity. The antiproliferative activity of ST004 formulations was assessed in murine (B16F10) and human (A375 and MNT-1) melanoma cell lines after 24 and 48 h incubation periods. The proof-of-concept of the antimelanoma properties of ST004 formulations was carried out in subcutaneous and metastatic murine melanoma models. Results: the developed liposomal formulations showed a low mean size (around 100 nm), high homogeneity (with a low polydispersity index) and high incorporation efficiency (51 ± 15%). ST004 formulations exhibited antiproliferative activity with EC50 values in the μmolar range being cell-line- and incubation-period-dependent. On the opposite side, the benchmark antimelanoma compound, dacarbazine (DTIC), presented an EC50 > 100 μM. Cell cycle analysis revealed an arrest in G0/G1 phase for Free-ST004 in all cell lines. In turn, LIP-ST004 led to a G0/G1 halt in B16F10, and to an arrest in S phase in A375 and MNT-1 cells. Preliminary mechanistic studies in human red blood cells suggest that gold-based inhibition of glycerol permeation acts through aquaglyceroporin 3 (AQP3). In a metastatic murine melanoma, a significant reduction in lung metastases in animals receiving LIP-ST004, compared to free gold complex and DTIC, was observed. Conclusion: This study highlights the antimelanoma potential of a new gold-based complex. Additional studies, namely in vivo biodistribution profile and therapeutic validation of this organogold complex in other melanoma models, are expected to be performed in further investigations.
Combined Effects of Deficit Irrigation and Biostimulation on Water Productivity in Table Grapes
Authors: Zapata-García Susana , Temnani Abdelmalek , Berríos Pablo , Marín-Durán Laura , Espinosa Pedro J. , Monllor Claudia , Pérez-Pastor Alejandro
Published: December 2024

Biostimulation and precision irrigation are strategies that increase the sustainability of agriculture, and both have been widely studied in table grapes, but their interaction is a new approach for viticulture. The objective of this field trial was to assess the physiological effects of water deficit on table grapes pretreated for two consecutive years with five different biostimulation programs. Therefore, during the first year, vines were preconditioned with biostimulants composed of microorganisms, seaweed, and plant extracts and compared to an untreated control. During the second year, the same biostimulation treatments were evaluated under two different irrigation schedules: (i) farmer irrigation (FI), according to a farmer’s criteria; and (ii) a deficit irrigation program, precision irrigation (PI), in which irrigation water was reduced from the post-veraison period to harvest, setting a threshold for allowable soil water depletion of 10% with respect to field capacity in order to minimize water leaching. The water inputs in the treatments under PI were reduced by 30% with respect to the FI treatment. While the deficit irrigation treatment clearly affected the plant water status indicators, biostimulation enhanced the root colonization by mycorrhizae and showed a trend of increased new root density. The combined effect of biostimulation and PI was shown to be an efficient strategy for optimizing the available resources, promoting the yield precocity.
Leaf Aquaporin Expression in Grafted Plants and the Influence of Genotypes and Scion/Rootstock Combinations on Stomatal Behavior in Grapevines Under Water Deficit
Authors: Galaz Andrea , Pérez-Donoso Alonso G. , Gambardella Marina
Published: December 2024

This study investigates the impact of water stress on grapevines, specifically examining the role of rootstocks and aquaporins. Two experiments on potted plants were conducted in central Chile during the summer, under conditions of high water demand, involving various rootstock genotypes and combinations of Cabernet Sauvignon (CS) grafted onto rootstocks. Significant differences were observed among plants in terms of stem water potential, stomatal conductance, and growth rate. Notably, the CS/CS combination consistently displayed the slowest growth rate, regardless of the irrigation treatment. The study also analyzed the expression levels of plasma membrane intrinsic protein (PIP) and tonoplast intrinsic protein (TIP) aquaporins in the leaves of grafted plants. Specifically, VvPIP2;2 aquaporins showed reduced expression after 14 days without irrigation, whereas VvTIP1;1 and VvTIP2;1 expression levels correlated positively with gs responses in grafted plants, suggesting their role in modulating water content in leaves under water stress. TIP aquaporins likely play a significant role in the differential responses of CS plants towards near-isohydric or anisohydric behavior. The CS/CS combination exhibited near-isohydric behavior, correlating with lower TIP aquaporin expression, while the combination of CS onto 1103P and 101-14 showed higher expression, indicating anisohydric behavior. The findings suggest that grafted plants are more resilient to water stress, supporting the idea that rootstocks can mitigate the effects of water stress on the scion.
Control of Green Mold and Sour Rot in Mandarins by Postharvest Application of Natamycin and an Allium Extract
Authors: Faggiani Elena Pérez , Fernandez Gerónimo , Cocco Mariángeles , Sbres Mauricio , Blanco Oribe , Lado Joanna
Published: December 2024

The efficacy of natamycin (Fruitgard Nat 20) and Proallium (an extract of allium including propyl thiosulfinate oxide (PTSO)) against sour rot and green mold in mandarins was evaluated under controlled and commercial conditions. The study involved artificial inoculation of Nova, Tango, Orri, Afourer, Murcott, and Nules Clementine mandarins with isolates of Penicillium digitatum resistant to imazalil and pyrimethanil and an isolate of Geotrichum citri-aurantii susceptible to propiconazole fungicides. Under laboratory conditions, natamycin applied at 1500 µg mL−1 significantly reduced green mold by 61.2% in Orri and sour rot by 62.8% in Nova and 80% in Tango. Increasing the concentration to 2000 µg mL−1 further improved control of sour rot in Nova to 92.8%. In commercial trials, natamycin at 1500 µg mL−1 was ineffective on Afourer; however, 2000 µg mL−1 reduced sour rot by 39% on Nules Clementine. Proallium (12–16 µg mL−1 PTSO) applied under controlled conditions effectively reduced green mold by 33% in Nova and 31% in Nules Clementine, and sour rot by 19%, 41%, and 36% in Nules Clementine, Nova, and Afourer, respectively. Under commercial conditions, using the same dose of Proallium, there was a 51.5% reduction in the incidence of imazalil and pyrimethanil-resistant P. digitatum strains and a 36.5% reduction in sour rot. Both natamycin and PTSO showed promising results for managing green mold caused by fungicide-resistant strains, but further research is needed to optimize control of sour rot in mandarins.
A Virulence Factor from Sclerotinia sclerotiorum Targets the Host Chloroplast Proteins to Promote Infection
Authors: Cui Wenjing , Xiao Kunqin , Yang Feng , Qiao Kaibin , Xu Xun , Gu Songyang , Guo Jinxin , Song Zhuojian , Pan Hongyu , Wang Fengting , Zhang Yanhua , Liu Jinliang
Published: December 2024

Chloroplasts are not only places for photosynthesis, but also participate in plant immunity and are important targets of pathogens. Pathogens secrete chloroplast-targeted proteins (CTPs) that disrupt host immunity and promote infection. Sclerotinia sclerotiorum (Lib.) de Bary is a phytopathogenic fungus with a broad host range. However, little is known about the pathogenic mechanisms underlying this wide host range. In this study, we investigated the role of Chloroplast-Targeted Protein 1 (SsCTP1) secreted by S. sclerotiorum in pathogenesis, which inhibits plant immunity and promotes pathogen infections. SsCTP1 was highly up-regulated during the early stages of S. sclerotiorum infection in various hosts, and its transient expression in Nicotiana benthamiana revealed that it was predominantly localized within chloroplasts. Mutants with SsCTP1 deletion exhibited a similar growth rate and colony morphology to the wild type, but significantly reduced pathogenicity in various hosts. Moreover, SsCTP1 inhibited chitin-induced callose deposition and defense gene expression, and enhanced sensitivity to S. sclerotiorum in N. benthamiana. Similarly, transgenic Arabidopsis thaliana overexpressing SsCTP1 displayed an increased susceptibility to S. sclerotiorum. Furthermore, two host proteins that interact with SsCTP1, Coproporphyrinogen-III oxidase (GmCPX), and shikimate kinase 2 (GmSKL2) were identified by screening the soybean cDNA library, and these interactions were confirmed in vivo. Importantly, the silencing of NbCPX by virus-induced gene silencing enhanced N. benthamiana resistance to S. sclerotiorum. Our results indicate that SsCTP1 is an important pathogenic factor that contributes to the wide host range of S. sclerotiorum and may inhibit plant immunity by targeting the chloroplast proteins GmCPX and GmSKL2, which are ubiquitous in host plants.
A Bifunctional Nuclease Promotes the Infection of Zucchini Yellow Mosaic Virus in Watermelon by Targeting P3
Authors: Kang Baoshan , Liu Lifeng , Liu Liming , Liu Mei , Wu Huijie , Peng Bin , Liang Zhiling , Liu Fengnan , Zang Yaoxing , Gu Qinsheng
Published: December 2024

Potyviral P3 is involved in viral replication, movement, and pathogenicity; however, its biochemical function is unknown. In this study, the P3 of the zucchini yellow mosaic virus (ZYMV) interacted with ClBBD, a protein with high ortholog bifunctional nuclease activity, in watermelon. The binding site was shown via yeast two-hybrid screening and BiFC assay to be located at the N-terminus of P3 rather than P3N-PIPO. ClBBD localized predominantly to the chloroplast and plasma membrane. ZYMV P3 was also present in the nucleus and cytoplasm as aggregates. When co-expressed with P3 in tobacco, ClBBD formed aggregates with P3 in the cytoplasm. The knockdown of ClBBD using the VIGS vector pV190 and challenge with ZYMV revealed a positive correlation between viral accumulation and ClBBD expression, indicating that ClBBD reduces the resistance of watermelon to ZYMV. Furtherly, we found that when P3 and ClBBD were transiently co-expressed in tobacco, the level of P3 was significantly higher than that when it was expressed alone or co-expressed with GUS. It inferred that ClBBD may be able to stabilize the expression of P3. Overall, the results suggest that the interaction of P3 with ClBBD promotes virus infection, and ClBBD may be involved in stabilizing the expression level of P3.
Effects of Hygrothermal Condition on Water Diffusion and Flexural Properties of Carbon–Glass Hybrid Fiber-Reinforced Epoxy Polymer Winding Pipes
Authors: Zhao Ying , Li Qiang , Zhou Guoqiang , Zhu Kehai , Jing Bo , Zhu Kangnan , Shi Jiajun , Li Chenggao
Published: December 2024

Carbon–glass hybrid fiber-reinforced epoxy polymer (C-GFRP) winding pipes integrated with the advantages of light weight, high strength, corrosion resistance, and cost-effectiveness offer immense potential to mitigate corrosion issues in oil, gas, and water transportation pipelines. In this study, C-GFRP winding pipes underwent accelerated aging tests through immersion in distilled water at temperatures of 25 °C, 40 °C, and 60 °C for 146 days. Water absorption tests were conducted to investigate the water absorption behavior of only CFRP- or GFRP-side absorbed water. Bending tests were performed to assess the evolution of the pipes’ flexural properties in two directions (GFRP or CFRP in tension). The results showed that the single-sided water absorption behavior adhered to the two-stage diffusion model. The diffusion coefficient, activation energy, and 146-day water absorption were all higher for the CFRP-side absorbed water compared to the GFRP-side absorbed water. The flexural strength and modulus of C-GFRP pipes were influenced by post-curing and resin hydrolysis/debonding. Initially, the flexural strength of CFRP in tension was higher than that of CFRP in tension. After 146 days of aging, the flexural strength of CFRP in tension was lower than that of CFRP in tension. Utilizing Arrhenius theory, the long-term lives were predicted for the flexural strength at temperatures of 5.4 °C, 12.8 °C, and 17.8 °C. The predicted lives of GFRP in tension were higher than those of CFRP in tension.
Melanosis Contributing to Downgrading in Processed Broiler Chickens in Argentina: Preliminary Diagnostic Findings
Authors: Gornatti-Churria Carlos Daniel , Brambillasca Sebastián , García Patricio A.
Published: December 2024

Melanosis is scarcely reported as a potential cause of carcass downgrading in broilers worldwide. We described the preliminary diagnostic findings of dermal and celomic fat tissue melanosis in a total of four episodes of increased downgrading in 31-to-50-day-old male Cobb500TM broiler chicken carcasses at a processing plant in Argentina from November 2016 to March 2022. Veterinary inspectors evaluated and sampled a total of 10 carcasses. Gross findings included the gray discoloration of subcutaneous tissue in wings and legs, celomic fat tissue and peritoneum. Microscopically, melanocytes infiltrated the connective tissue and adipose cells of subcutaneous tissue in the skin of legs and wings as well as the celomic fat tissue of all 10 birds. The Fontana–Masson stain revealed black melanin granules in subcutaneous tissue and celomic fat tissue, demonstrating its value as a histologic tool to confirm grossly compatible melanosis in all of the evaluated tissues sampled at the broiler processing plant. We propose to add melanosis as one of the less frequently and rare diagnosed cutaneous darkening conditions which potentially contributes to downgrading in chicken processing plants globally.
Correction: Irvine et al. Transpersonal Ecodelia: Surveying Psychedelically Induced Biophilia. Psychoactives 2023, 2, 174–193
Authors: Irvine Alexander , Luke David , Harrild Freya , Gandy Sam , Watts Rosalind
Published: December 2024

The authors would like to make the following corrections to this published paper [...]
Teacher Training for Religious Education in Türkiye: Policy, Debate, and Diversity
Authors: Hendek Abdurrahman , Zengin Mahmut
Published: December 2024

Teacher education is debated all over the world, and a part of this debate concerns the quality of teacher education. For religious education (RE) teacher education, however, the debate is not only about quality but also about growing diversity. In this article, we explore how RE teachers have been educated and how this has changed in Türkiye. How religious diversity has been integrated into the RE teacher education system is also explored. By teacher education/training, we mean initial (not in-service) teacher education, and by RE, we mean different types of RE provided in schools in Türkiye: compulsory, optional, and vocational RE. The findings reveal that there have been frequent changes, back-and-forth reforms, and a lack of RE teacher education philosophy in Türkiye. Therefore, establishing a consistent RE teacher education policy remains a serious challenge. Moreover, it seems that religious diversity has not been well integrated into RE teacher education in Türkiye. Currently, theology faculties only offer two courses which are overtly related to religious diversity, and both of them approach religious diversity from a historical perspective. It seems that there is a need to rethink the program structures of theology faculties to reform RE teacher education. Theology faculties may offer several programs in line with employment areas. In this way, religious diversity might also become better integrated into compulsory RE teacher education programs. However, this process should be carried out with a scientific understanding by conducting stakeholder and needs analyses.
Sustainable Development in Islamic Theology: From Occasion-Driven Approaches to a Comprehensive Understanding of Sustainability Using the Example of Water-Related Fatwas in Jordan
Authors: Abd-Elsalam Ahmed M. F. , Binay Sara
Published: December 2024

The Islamic environmental theology (IET) that emerged in the 20th century is analyzed and two main strands are pursued: the efforts to protect the environment derived from the thinking of some Muslim scholars and activists in Europe and North America, and the discourse of Muslim theologians in Arab countries such as Egypt and Jordan that developed in the face of environmental destruction and associated problems in the societies concerned. The topic of water, i.e., the Sustainable Development Goal (SDG) of clean water and access to sanitation, played a particularly important initial role in the discourse in Arab countries. Jordan, for example, is one of the most water-scarce countries in the world and, since 2010, various fatwas have been seen, such as expert opinions from the General Iftaa’ Department, the state authority for Islamic jurisprudence in Jordan, on the use of water. The discourse of Muslim jurists on the Sustainable Development Goals in general has developed from this. At the same time, it is obvious that the normative theology in Arab countries is more reactive than progressive concerning the challenges of climate change. This article comes to the conclusion that Islamic theology, even if it starts from the same resources of tradition, comes to form different discourses depending on the regional context. This study will show how institutions of Islamic law respond to climate change and what flexibility is inherent in Islamic theology to meet these challenges.
Carbon Footprint, Financial Structure, and Firm Valuation: An Empirical Investigation
Authors: Hágen István , Ahmed Amanj Mohamed
Published: December 2024

This study aims to investigate the complex link between carbon emissions, firm value, and financial choice in regard to the GCC, a dynamic emerging economy. It also seeks to answer the question on whether the financial structure of a firm moderates the correlation between carbon emissions and firm value. We focus on analyzing data from non-financial firms registered on the GCC stock markets between 2010 and 2020. By applying the GLS technique, we assess the impact of carbon emissions on firm value and examine the manner in which a firm’s financial structure either enhances or hinders this relationship. The results demonstrate that there is a strong and adverse connection between carbon emissions and corporate value, as increased emissions translate into lower corporate value. The study then moves on to emphasize the critical role that capital financing plays in mitigating the detrimental effects of carbon emissions. This is accomplished by balancing both debt and equity in terms of their proper proportions (optimal capital structure). However, excessive borrowing could have adverse consequences in terms of carbon emissions on company value. Moreover, the GMM estimator is also applied to carry out a robustness check and the results are consistent with the main findings. This study highlights the significance of financial strategy in advancing sustainability and protecting business value. These findings are supported by both stakeholder and signaling theory, proving that companies can use their capital financing to signal their dedication to sustainability. These results could be used by GCC policymakers to create rules and regulations that encourage environmentally friendly corporate activities and efforts to lower emissions. The research expands the existing literature by examining the difficulties and opportunities faced by GCC firms when combining financial strategy with environmental objectives. It may be necessary to perform additional research in regard to various circumstances and for an extended period, because this study is restricted to non-financial sectors.
Ensemble Learning for Oat Yield Prediction Using Multi-Growth Stage UAV Images
Authors: Zhang Pengpeng , Lu Bing , Shang Jiali , Wang Xingyu , Hou Zhenwei , Jin Shujian , Yang Yadong , Zang Huadong , Ge Junyong , Zeng Zhaohai
Published: December 2024

Accurate crop yield prediction is crucial for optimizing cultivation practices and informing breeding decisions. Integrating UAV-acquired multispectral datasets with advanced machine learning methodologies has markedly refined the accuracy of crop yield forecasting. This study aimed to construct a robust and versatile yield prediction model for multi-genotyped oat varieties by investigating 14 modeling scenarios that combine multispectral data from four key growth stages. An ensemble learning framework, StackReg, was constructed by stacking four base algorithms—ridge regression (RR), support vector machines (SVM), Cubist, and extreme gradient boosting (XGBoost)—to predict oat yield. The results show that, for single growth stages, base models achieved R2 values within the interval of 0.02 to 0.60 and RMSEs ranging from 391.50 to 620.49 kg/ha. By comparison, the StackReg improved performance, with R2 values extending from 0.25 to 0.61 and RMSEs narrowing to 385.33 and 542.02 kg/ha. In dual-stage and multi-stage settings, the StackReg consistently surpassed the base models, reaching R2 values of up to 0.65 and RMSE values as low as 371.77 kg/ha. These findings underscored the potential of combining UAV-derived multispectral imagery with ensemble learning for high-throughput phenotyping and yield forecasting, advancing precision agriculture in oat cultivation.