bluuebunny/crossref_metadata_2025 huggingface dataset:
Roles of GFAT and PFK genes in energy metabolism of brown planthopper, Nilaparvata lugens
Authors: Si Hui-Ru , Sun Si-Si , Liu Yong-Kang , Qiu Ling-Yu , Tang Bin , Liu Fang , Fu Qiang , Xu Cai-Di , Wan Pin-Jun
Published: June 2023

Glutamine:fructose-6-phosphate aminotransferases (GFATs) and phosphofructokinase (PFKs) are the principal rate-limiting enzymes involved in hexosamine biosynthesis pathway (HBP) and glycolysis pathway, respectively. In this study, the NlGFAT and NlPFK were knocked down through RNA interference (RNAi) in Nilaparvata lugens, the notorious brown planthopper (BPH), and the changes in energy metabolism were determined. Knockdown of either NlGFAT or NlPFK substantially reduced gene expression related to trehalose, glucose, and glycogen metabolism pathways. Moreover, trehalose content rose significantly at 72 h after dsGFAT injection, and glycogen content increased significantly at 48 h after injection. Glucose content remained unchanged throughout the experiment. Conversely, dsPFK injection did not significantly alter trehalose, but caused an extreme increase in glucose and glycogen content at 72 h after injection. The Knockdown of NlGFAT or NlPFK significantly downregulated the genes in the glycolytic pathway, as well as caused a considerable and significant decrease in pyruvate kinase (PK) activity after 48 h and 72 h of inhibition. After dsGFAT injection, most of genes in TCA cycle pathway were upregulated, but after dsNlPFK injection, they were downregulated. Correspondingly, ATP content substantially increased at 48 h after NlGFAT knockdown but decreased to an extreme extent by 72 h. In contrast, ATP content decreased significantly after NlPFK was knocked down and returned. The results have suggested the knockdown of either NlGFAT or NlPFK resulted in metabolism disorders in BPHs, highlighting the difference in the impact of those two enzyme genes on energy metabolism. Given their influence on BPHs energy metabolism, developing enzyme inhibitors or activators may provide a biological control for BPHs.
SbWRKY75- and SbWRKY41-mediated jasmonic acid signaling regulates baicalin biosynthesis
Authors: Fang Shiyuan , Zhang Chen , Qiu Shi , Xiao Ying , Chen Kaixian , Lv Zongyou , Chen Wansheng
Published: June 2023

Introduction

Scutellaria baicalensis Georgi is a traditional Chinese medicinal plant with broad pharmacological activities whose main active ingredient is the flavonoid baicalin. Given its medicinal value and increasing market demand, it is essential to improve the plant’s baicalin content. Flavonoid biosynthesis is regulated by several phytohormones, primarily jasmonic acid (JA).

Methods

In this study, we conducted transcriptome deep sequencing analysis of S. baicalensis roots treated with methyl jasmonate for different durations (1, 3, or 7 hours). Leveraging weighted gene co-expression network analysis and transcriptome data, we identified candidate transcription factor genes involved in the regulation of baicalin biosynthesis. To validate the regulatory interactions, we performed functional assays such as yeast one-hybrid, electrophoretic mobility shift, and dual-luciferase assays.

Results

Our findings demonstrated that SbWRKY75 directly regulates the expression of the flavonoid biosynthetic gene SbCLL-7, whereas SbWRKY41 directly regulates the expression of two other flavonoid biosynthetic genes, SbF6H and SbUGT, thus regulating baicalin biosynthesis. We also obtained transgenic S.baicalensis plants by somatic embryo induction and determined that overexpressing SbWRKY75 increased baicalin content by 14%, while RNAi reduced it by 22%. Notably, SbWRKY41 indirectly regulated baicalin biosynthesis by modulating the expression of SbMYC2.1, SbJAZ3 and SbWRKY75.

Discussion

This study provides valuable insights into the molecular mechanisms underlying JA-mediated baicalin biosynthesis in S. baicalensis. Our results highlight the specific roles of transcription factors, namely SbWRKY75 and SbWRKY41, in the regulation of key biosynthetic genes. Understanding these regulatory mechanisms holds significant potential for developing targeted strategies to enhance baicalin content in S. baicalensis through genetic interventions.
The moderating role of hair cortisol in the association of early and recent stress with stress-related phenotypes
Authors: Torrecilla Pilar , Barrantes-Vidal Neus
Published: June 2023

Background

Increased hair cortisol concentrations (HCC) have been found in clinical samples of schizophrenia, first episode psychosis and clinical risk for psychosis, but evidence of such is scarce in schizotypy. High HCC are supposed to reflect elevated chronic stress. However, HCC were not directly associated with adversity measures and stress-related phenotypes in previous research. This study tested whether HCC moderated the association between a comprehensive range of psychosocial stressors with several stress-related phenotypes in a sample of nonclinical young adults. It was expected that stressors, either distal (i.e., early-life) or recent, would be associated with subclinical features, particularly for those with elevated HCC, reflecting the effects of a potential biological sensitization to stress.

Methods

The sample comprised 132 nonclinical young adults belonging to the Barcelona Longitudinal Investigation of Schizotypy Study (BLISS). Participants completed a questionnaire of childhood adversity and two complementary measures of recent life events, tapping threatening vs. more general life events. Both the frequency and subjective impact (positive vs. negative) of general life events were also assessed. Psychotic (i.e., schizotypy, suspiciousness) and non-psychotic (i.e., depression, anxiety) subclinical features as well as appraisals of perceived stress were examined. Hierarchical linear regressions and simple slope analyses were computed.

Results

HCC moderated the effects of both early and recent stress on suspiciousness as well as the effects of recent life events on perceived stress, such that those with higher HCC presented increased suspiciousness and perceived stress at higher levels of stress exposure. Positive, but not negative, recent life events were associated with decreased perceived stress and depression, and these associations were moderated by low HCC, indicating a buffering effect for those with a non-impaired HPA axis.

Conclusion

In line with the neural diathesis-stress model, results highlight the role of the interplay between the HPA axis and exposure to stressful experiences in exacerbating psychosis features and extend evidence to the nonclinical expression of the psychosis continuum. In addition, findings support the protective effect of positive experiences in decreasing stress appraisals and affective disturbances, which is consistent with emerging research about the relevance of positive factors in reducing the likelihood of psychopathological outcomes.
The effects of emotions on the disposition to normative and non-normative political action in the context of the Chilean post-social outburst
Authors: Hatibovic Fuad , Sandoval Juan , Faúndez Ximena , Gaete Jose-Manuel , Bobowik Magdalena , Ilabaca Paola
Published: June 2023

This article analyzes the role of Chileans’ emotions as predictors of normative and non-normative political action in the context of the post-social outbreak and the constituent process. We carried out three descriptive studies: first, a study conducted 1 year after the social outburst (n = 607), a second one carried out before the constitutional referendum (n = 320), and a third study conducted after the constitutional referendum (n = 210). The results indicated that participants present a higher disposition to normative over non-normative political action, although both lose strength as the studies temporally move away from the social outburst. Also, our research established that emotions directed towards different events related to the Chilean political process play a conspicuous role in predicting the disposition to mobilize in a normative and non-normative way.
Evaluating normalization accounts against the dense vowel space of Central Swedish
Authors: Persson Anna , Jaeger T. Florian
Published: June 2023

Talkers vary in the phonetic realization of their vowels. One influential hypothesis holds that listeners overcome this inter-talker variability through pre-linguistic auditory mechanisms that normalize the acoustic or phonetic cues that form the input to speech recognition. Dozens of competing normalization accounts exist—including both accounts specific to vowel perception and general purpose accounts that can be applied to any type of cue. We add to the cross-linguistic literature on this matter by comparing normalization accounts against a new phonetically annotated vowel database of Swedish, a language with a particularly dense vowel inventory of 21 vowels differing in quality and quantity. We evaluate normalization accounts on how they differ in predicted consequences for perception. The results indicate that the best performing accounts either center or standardize formants by talker. The study also suggests that general purpose accounts perform as well as vowel-specific accounts, and that vowel normalization operates in both temporal and spectral domains.
Writing motivation and ability profiles and transition during a technology-based writing intervention
Authors: Cruz Cordero Tania , Wilson Joshua , Myers Matthew C. , Palermo Corey , Eacker Halley , Potter Andrew , Coles Jessica
Published: June 2023

Students exhibit heterogeneity in writing motivation and ability. Profiles based on measures of motivation and ability might help to describe this heterogeneity and better understand the effects of interventions aimed at improving students’ writing outcomes. We aimed to identify writing motivation and ability profiles in U.S. middle-school students participating in an automated writing evaluation (AWE) intervention using MI Write, and to identify transition paths between profiles as a result of the intervention. We identified profiles and transition paths of 2,487 students using latent profile and latent transition analysis. Four motivation and ability profiles emerged from a latent transition analysis with self-reported writing self-efficacy, attitudes toward writing, and a measure of writing writing: Low, Low/Mid, Mid/High, and High. Most students started the school year in the Low/Mid (38%) and Mid/High (30%) profiles. Only 11% of students started the school year in the High profile. Between 50 and 70% of students maintained the same profile in the Spring. Approximately 30% of students were likely to move one profile higher in the Spring. Fewer than 1% of students exhibited steeper transitions (e.g., from High to Low profile). Random assignment to treatment did not significantly influence transition paths. Likewise, gender, being a member of a priority population, or receiving special education services did not significantly influence transition paths. Results provide a promising profiling strategy focused on students’ attitudes, motivations, and ability and show students’ likeliness to belong to each profile based on their demographic characteristics. Finally, despite previous research indicating positive effects of AWE on writing motivation, results indicate that simply providing access to AWE in schools serving priority populations is insufficient to produce meaningful changes in students’ writing motivation profiles or writing outcomes. Therefore, interventions targeting writing motivation, in conjunction with AWE, could improve results.
Hidden faces, altered perceptions: the impact of face masks on interpersonal perception
Authors: Wang Shuai , Han Chengyang , Sang Zihan , Zhang Xuhui , Chen Shitao , Wang Haoran , Wang Gang , Xu Yiqian , Lei Xue , Chen Jixu
Published: June 2023

The pandemic has made wearing masks commonplace, prompting researchers to investigate their effects on interpersonal perception. Findings indicate masks obstruct face identification and expression recognition, with lower face cues being most affected. When judging attractiveness, masks can enhance the appeal of less attractive faces, but reduce the appeal of more attractive faces. Trust and speech perception outcomes are inconclusive. Future studies could focus on individual differences in how masks influence our perception of others.
Depressive symptoms and associated factors among pregnant women attending antenatal care at Comprehensive Specialized Hospitals in Northwest Ethiopia, 2022: an institution-based cross-sectional study
Authors: Takelle Girmaw Medfu , Nakie Girum , Rtbey Gidey , Melkam Mamaru
Published: June 2023

Background

Depression during pregnancy has a significant impact on public health as it can adversely affect both the mother's and the child's health. These can have devastating effects on the mother, the unborn child, and the entire family.

Objective

This study aimed to determine the prevalence of depressive symptoms and associated factors among pregnant women in Ethiopia.

Method

An institutional-based cross-sectional study was conducted among pregnant women attending antenatal care services at comprehensive specialized hospitals in Northwest Ethiopia from May to June 2022.

Measurement

The desired data were collected through face-to-face interview techniques by using validated questionnaires such as the Edinburgh Postnatal Depression Scale, the Oslo-3 social support scale, and the Abuse Assessment Screen tools. The data were analyzed by using SPSS Version 25. Logistic regression analysis was used to identify factors associated with antenatal depressive symptoms. Variables having a p-value of <0.2 in the bivariate analysis were entered into the multivariable logistic regression. A p-value of <0.05 was considered statistically significant, at 95% CI.

Results

This study revealed that 91 (19.2%) pregnant women screened positive for depressive symptoms. According to multivariable logistic regression, living in rural areas (adjusted odds ratio (AOR) = 2.58, 95% CI: 1.267, 5.256), being in the second or third trimesters of gestational phase (AOR = 4.40, 95% CI: 1.949, 9.966 and AOR = 5.42, 95% CI: 2.438, 12.028, respectively), having a history of alcohol use (AOR = 2.41, 95% CI: 1.099, 5.260), having moderate or poor social support (AOR = 2.55, 95% CI: 1.220, 5.338 and AOR = 2.41, 95% CI: 1.106, 5.268), and having a history of intimate partner violence (AOR = 2.67, 95% CI: 1.416, 5.016) were the factors significantly associated with depressive symptoms at a p-value of ≤ 0.05.

Conclusion and recommendation

The prevalence of depressive symptoms among pregnant women was high. Living in rural areas, second and third trimesters, use of alcohol, having moderate to poor social support, and having a history of intimate partner violence were variables significantly associated with depressive symptoms during pregnancy.
Social robotics to support older people with dementia: a study protocol with Paro seal robot in an Italian Alzheimer’s day center
Authors: Bevilacqua Roberta , Maranesi Elvira , Felici Elisa , Margaritini Arianna , Amabili Giulio , Barbarossa Federico , Bonfigli Anna Rita , Pelliccioni Giuseppe , Paciaroni Lucia
Published: June 2023

Introduction

The aging of the population and the high incidence of those over 80 lead to an inevitable increase in chronic degenerative diseases, such as dementia, resulting in increased morbidity and disability. Treatment of people with dementia involves both pharmacological and non-pharmacological interventions. In particular, robot-assisted therapy is a potentially useful treatment for dementia as it has the advantage of improving mood, encouraging social interaction and communication. The overall objective of the study is to evaluate the improvement in patient-perceived quality of life following the use of the Paro robot integrated with usual care in the older people with dementia.

Methods and analysis

For this study, 20 patients with dementia are recruited and divided into Experimental Group (EG) and Control Group (CG). Twenty-four session of intervention are conducted, divided into 2 sessions per week, for 12 weeks. The therapy sessions last 20 min. The Experimental Group will receive a social robotic intervention with Paro combined with usual care; the Control Group will receive only the traditional therapy, consisting of cognitive stimulation (reality orientation therapy, cognitive training) and occupational activities (painting workshops, cooking workshops, garden therapy, music therapy, etc.). Paro is a seal-shaped robot designed to have a calming effect and elicit emotional responses in patients in hospitals, nursing homes, and retirement homes. Assessment will be performed at the baseline, at the end of intervention and 3 months after the end of intervention. During these phases, several scales will be administered to the patients, such as Quality of Life—Alzheimer’s Disease, Addenbrooke’s Cognitive Examination, the Rating Anxiety In Dementia scale and the Cornell Scale for Depression in Dementia, Quebec User Evaluation of Satisfaction, Neuropsychiatric Inventory, the Technology Acceptance Model.

Discussions

The final goals of the present study are to evaluate the improvement in patient-perceived quality of life following the use of the Paro robot integrated with usual care in the older people with dementia.

Ethics and dissemination

The study was approved by the Ethic Committee of the Istituto Nazionale Ricovero e Cura Anziani, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS INRCA) during the session of 12 April 2022. It was recorded in ClinicalTrials.gov on 23 November 2022 on the number NCT05626205. The study findings will be used for publication in peer-reviewed scientific journals and presentations in scientific meetings.
Performance comparison of bio-inspired and learning-based clustering analysis with machine learning techniques for classification of EEG signals
Authors: Prabhakar Sunil Kumar , Won Dong-Ok
Published: June 2023

A comprehensive analysis of an automated system for epileptic seizure detection is explained in this work. When a seizure occurs, it is quite difficult to differentiate the non-stationary patterns from the discharges occurring in a rhythmic manner. The proposed approach deals with it efficiently by clustering it initially for the sake of feature extraction by using six different techniques categorized under two different methods, e.g., bio-inspired clustering and learning-based clustering. Learning-based clustering includes K-means clusters and Fuzzy C-means (FCM) clusters, while bio-inspired clusters include Cuckoo search clusters, Dragonfly clusters, Firefly clusters, and Modified Firefly clusters. Clustered values were then classified with 10 suitable classifiers, and after the performance comparison analysis of the EEG time series, the results proved that this methodology flow achieved a good performance index and a high classification accuracy. A comparatively higher classification accuracy of 99.48% was achieved when Cuckoo search clusters were utilized with linear support vector machines (SVM) for epilepsy detection. A high classification accuracy of 98.96% was obtained when K-means clusters were classified with a naive Bayesian classifier (NBC) and Linear SVM, and similar results were obtained when FCM clusters were classified with Decision Trees yielding the same values. The comparatively lowest classification accuracy, at 75.5%, was obtained when Dragonfly clusters were classified with the K-nearest neighbor (KNN) classifier, and the second lowest classification accuracy of 75.75% was obtained when Firefly clusters were classified with NBC.
Collaborative leadership in team science: dynamics of sense making, decision making, and action taking
Authors: Jiang Gemma
Published: June 2023

To help cross-disciplinary science teams navigate through internal and external complexities, this perspective article explores the application of three conceptual frameworks: Theory U, Divergence-Convergence Diamond, and Strategic Doing. These frameworks enable science teams to avoid common pitfalls by operationalizing collaborative leadership as iterative cycles of distributed sense making, decision making and action taking. Implications for team science practices include facilitating the process, prototyping the future and applying dynamic roles and responsibilities.
Migration and education on social media: what migrants discuss about education in Facebook groups
Authors: Dedecek Gertz Helena
Published: June 2023

Drawing from a networked perspective of migration and from the concept of “transnational education,” this paper investigates the education-related topics discussed in Facebook groups among Brazilian migrants in Germany. The paper examines the “latent ties” activated in migrant Facebook groups as part of networks that can be used to collect information about migratory pathways involving educational opportunities. A qualitative content analysis was conducted with 2.297 posts retrieved from six Facebook groups divided into location, vocational education and training (VET), and professional groups. The outcomes point out that there is a demand for transnational education beyond university degrees. Additionally, the paper highlights that latent ties can be used to collect and cross-check information in migratory contexts involving education.
The genetic network underlying the evolution of pathogenicity in avian Escherichia coli
Authors: Palmieri Nicola , Apostolakos Ilias , Paudel Surya , Hess Michael
Published: June 2023

Introduction

Colibacillosis is a worldwide prevalent disease in poultry production linked to Escherichia coli strains that belong to the avian pathogenic E. coli (APEC) pathotype. While many virulence factors have been linked to APEC isolates, no single gene or set of genes has been found to be exclusively associated with the pathotype. Moreover, a comprehensive description of the biological processes linked to APEC pathogenicity is currently lacking.

Methods

In this study, we compiled a dataset of 2015 high-quality avian E. coli genomes from pathogenic and commensal isolates, based on publications from 2000 to 2021. We then conducted a genome-wide association study (GWAS) and integrated candidate gene identification with available protein-protein interaction data to decipher the genetic network underlying the biological processes connected to APEC pathogenicity.

Results

Our GWAS identified variations in gene content for 13 genes and SNPs in 3 different genes associated with APEC isolates, suggesting both gene-level and SNP-level variations contribute to APEC pathogenicity. Integrating protein-protein interaction data, we found that 15 of these genes clustered in the same genetic network, suggesting the pathogenicity of APEC might be due to the interplay of different regulated pathways. We also found novel candidate genes including an uncharacterized multi-pass membrane protein (yciC) and the outer membrane porin (ompD) as linked to APEC isolates.

Discussion

Our findings suggest that convergent pathways related to nutrient uptake from host cells and defense from host immune system play a major role in APEC pathogenicity. In addition, the dataset curated in this study represents a comprehensive historical genomic collection of avian E. coli isolates and constitutes a valuable resource for their comparative genomics investigations.
Factors Associated With Delayed and Late Initiation of Antiretroviral Therapy Among Patients With HIV in Beijing, China, 2010–2020
Authors: Mi Yuanqi , Zhou Mengge , Zeng Yuhong , Wang Peicheng , Gao Liangmin , Cheng Feng
Published: June 2023

Objectives: To determine factors associated with late and delayed antiretroviral therapy (ART) initiation in China and provide evidence for HIV prevention.

Methods: Logistics regression model was used to determine factors associated with three outcomes: late (CD4 cell count <200 cells/µL or clinical AIDS diagnosis prior to ART initiation), delayed (more than 1 month between HIV diagnosis date and ART initiation) and either late or delayed ART initiation.

Results: Multivariable analysis revealed that male, heterosexual, HIV diagnosis before 2014, HBV/HCV seropositive, and tuberculosis were associated with increased odds of all three outcomes. Conversely, married or cohabiting patients were less likely to have delayed ART initiation and either late or delayed ART initiation, while people who inject drugs were more likely to have these two outcomes. Additionally, older age was associated with an increased risk of having either late or delayed ART initiation, but a decreased risk of delayed ART initiation.

Conclusion: The proportion of late and delayed ART initiation decreased significantly after the release of the 2016 guidelines in China. To further improve late diagnosis and early treatment, precise interventions for key populations are required.
The 2nd International Symposium on New Frontiers in Reef Coral Biotechnology (12 May 2023, Taiwan)
Authors: Lin Chiahsin
Published: June 2023

For the second year in a row, the theme is “reef coral biotechnology”, specifically the interface between basic science and conservation. It has never been more important to attempt to leverage what we know about these beautiful, albeit highly imperiled and fragile, ecosystems towards conserving them. Our invited speakers’ areas of expertise span all levels of biological organization: from molecules within coral cells, to coral tissues, to entire coral colonies, and then up to reef-scale processes. Our goal is to promote communication not only among local Taiwanese marine biologists, but also those within Southeast Asia and farther afield; we especially encourage participation from early-career researchers, including Master’s students, PhD candidates, and post-doctoral researchers. It is our hope that the presentations (and the discussions that follow) will encourage collaboration. As importantly, we envision that the tools and approaches shared amongst us can be tapped into to expedite our collective efforts to better understand, manage, and conserve coral reefs.
CO2 Emission Allocation for Urban Public Buildings Considering Efficiency and Equity: An Application at the Provincial Level in China
Authors: Zhang Zhidong , Liu Yisheng , Du Zhuoqun
Published: June 2023

China is currently recognized as the leading global energy consumer and CO2 emitter. A significant amount of carbon emissions can be attributed to urban public buildings. Establishing an equitable and efficient carbon emission allocation mechanism is a crucial step to meeting the ambitious targets in China’s 2030 carbon peak plan. In this study, we estimate the total amount of CO2 emissions from urban public buildings by 2030 and propose a preliminary scheme of carbon quota assignment for each province. By means of applying the zero-sum gains data envelopment analysis (ZSG-DEA) model, the carbon emission quotas allocation of urban public buildings in China’s 30 provinces is proposed, and the corresponding pressure to reduce provincial carbon emissions is analyzed. The results indicate that Qinghai has the lowest carbon emission rate (0.01%) for urban public buildings, while Guangdong has the highest (9.06%). Among the provinces, Jiangsu, Jiangxi, and Tianjin face the least pressure in reducing carbon emissions from urban public buildings. On the other hand, Hebei, Beijing, and Anhui are under great pressure to decrease carbon emissions. Notably, Hebei is predicted to have the highest emission reduction requirement of 95.66 million tons. In terms of pressures on carbon emissions reduction for urban public buildings, Jiangsu, Jiangxi, and Tianjin exhibit the least pressure. Hebei, Beijing, and Anhui are facing intense pressure to decrease carbon emissions. These findings offer policymakers valuable insights into developing a fair and efficient carbon allowance allocation strategy, while also contributing to China’s efforts to mitigate carbon emissions and combat climate change.
E-Cadherin Expression Varies Depending on the Location within the Primary Tumor and Is Higher in Colorectal Cancer with Lymphoid Follicles
Authors: Markowski Adam R. , Ustymowicz Konstancja , Markowska Anna J. , Romańczyk Wiktoria , Guzińska-Ustymowicz Katarzyna
Published: June 2023

Reliable indicators of cancer advancement have actively been sought recently. The detection of colorectal cancer progression markers is essential in improving diagnostic and therapeutic protocols. The aim of the study was to investigate the profile of E-cadherin expression in colorectal cancer tissue depending on the TNM staging and its correlation with several clinical and histopathological features. The study included 55 colorectal cancer patients admitted to the surgical ward for elective surgery. Tissue samples were obtained from resected specimens. Different distributions of E-cadherin expression within tumors were observed; the highest percentage of positive E-cadherin expression was found in the invasive front and in the tumor center. Additionally, the different cellular distribution of E-cadherin expression was noticed; weak membranous E-cadherin expression was the highest in the invasive front and in the budding sites, but a strong membranous pattern was most frequent in the tumor center. Various distributions of E-cadherin expression depending on cancer progression were also found; E-cadherin expression in node-positive patients was lower in the tumor center and in the tumor invasive front, whereas, in patients with distant metastases, the expression of E-Cadherin was lower in the budding sites. In patients with higher TNM stages, E-cadherin expression was lower within the tumor (in the budding sites, tumor center, and invasive front). In tumors with lymphoid follicles, E-cadherin expression was higher in all localizations within the primary tumor. E-cadherin expression in the tumor center was also lower in tumors with some higher tumor budding parameters (areas of poorly differentiated components and poorly differentiated clusters). E-cadherin expression was found to be lower at the tumor center in younger individuals, at the budding sites in men, and at the surrounding lymph nodes in rectal tumors. Low E-cadherin expression appears to be a reliable indicator of higher cancer staging and progression. When assessing the advancement of cancer, apart from the TNM classification, it is beneficial to also consider the expression of E-cadherin. High tumor budding, the poverty of lymphoid follicles, and low E-cadherin expression analyzed simultaneously may contribute to a reliable assessment of colorectal cancer staging. These three histopathological features complement each other, and their investigation, together with conventional tumor staging and grading, may be very helpful in predicting the prognosis of colorectal cancer patients and qualifying them for the best treatment. The role of E-cadherin in the diagnosis and treatment of colorectal cancer, as a part of a personalized medicine strategy, still requires comprehensive, prospective clinical evaluations to precisely target the optimal therapies for the right patients at the right time.
Vascular Growth in Lymphomas: Angiogenesis and Alternative Ways
Authors: Ribatti Domenico , Tamma Roberto , Annese Tiziana , d’Amati Antonio , Ingravallo Giuseppe , Specchia Giorgina
Published: June 2023

The formation of new blood vessels is a critical process for tumor growth and may be achieved through different mechanisms. Angiogenesis represents the first described and most studied mode of vessel formation, but tumors may also use alternative ways to secure blood supply and eventually acquire resistance to anti-angiogenic treatments. These non-angiogenic mechanisms have been described more recently, including intussusceptive microvascular growth (IMG), vascular co-option, and vasculogenic mimicry. Like solid tumors, angiogenic and non-angiogenic pathways in lymphomas play a fundamental role in tumor growth and progression. In view of the relevant prognostic and therapeutic implications, a comprehensive understanding of these mechanisms is of paramount importance for improving the efficacy of treatment in patients with lymphoma. In this review, we summarize the current knowledge on angiogenic and non-angiogenic mechanisms involved in the formation of new blood vessels in Hodgkin’s and non-Hodgkin’s lymphomas.
Development of Structural Criteria for the Certification and Designation of Recreational and Therapeutic Forests in Bavaria, Germany
Authors: Immich Gisela , Robl Eva
Published: June 2023

Background: Based on the increasing evidence of forest on health, a research project assessed the structural certification criteria of recreational and therapeutic forests in 15 Bavarian pilot sites. The main project objectives were (1) to develop a certification standard for recreational and therapeutic forests and (2) to establish a certification and designation process. Methods: Relevant criteria were identified by a literature review. The findings, in conjunction with landscape planning principles, were used to develop a catalog of certification criteria, which was then tested and amended in field testing in the pilot sites. Results: The criteria catalog for recreational and therapeutic forests was structured into four sections: general requirements, forest-specific requirements, safety and infrastructure, and preventive and/or therapeutic interventions. At 13 pilot sites, 13 recreational forests and 3 therapeutic forests were designated, 47 professionals were trained, the designation process and a certification body were established, and the results were published in a manual (BayKK KuH). Conclusions: This newly developed Bavarian certification standard for recreational and therapeutic forests represents an innovative synthesis of science and empirical on-site experience. It enables interested parties to develop a recreational or therapeutic forest on the basis of transparently published standards in order to use the health-promoting effects of the forest in prevention and therapy.
Modeling Dynamics in Land Use and Land Cover and Its Future Projection for the Amazon Biome
Authors: Barbosa de Souza Kaíse , Rosa dos Santos Alexandre , Macedo Pezzopane José Eduardo , Machado Dias Henrique , Ferrari Jéferson Luiz , Machado de Oliveira Peluzio Telma , Toledo João Vitor , Freire Carvalho Rita de Cássia , Rizzo Moreira Taís , França Araújo Emanuel , Gomes da Silva Rosane , Pósse Senhorelo Adriano , Azevedo Costa Gizely , Duarte Nader Mardeni Vinícius , Horn Kunz Sustanis , Cordeiro dos Santos Elaine
Published: June 2023

The objectives were to analyze the dynamics of land use and land cover of the Amazon biome over time through spatial modeling, and project its future scenario with the Land Change Modeler (LCM) module. This analysis was based on 1985, 2014 and 2017 land cover data from the MapBiomas project, which was associated with socioeconomic explanatory variables based on the Cramer-V test. Results showed that the Forest Formation class occupied 3,844,800.75 km2 (91.20%) in 1985, and in 2014, there was a reduction to 3,452,129.25 km2 (81.89%). The pasture class had an initial area of 71,046.50 km2 (1.69%), and in 2014, there was an expressive increase to 437,670.00 km2 (10.38%). The analysis made it possible to verify that Forest Formation and Pastures were the classes that suffered the most changes, followed by the Annual and Perennial Culture and Mosaic of Agriculture and Pasture. The projected land use and coverage for 2044 suggests that there will be a reduction in Forest Formation due to a significant increase in the Pasture class. The simulations foreseen in this work are an important tool that can provide subsidies for supporting territorial planning in the region, public policies, and encouragement of best practices with a reduced impact in pasture areas.
Cloning, Bioinformatics Analysis and Physiological Function of the Pine Wood Nematode Bxadh2 Gene
Authors: Shen Luyang , Hu Jiafeng , Chen Yangxue , Tan Jiajin , Ye Jianren
Published: June 2023

In previous research, the pine wood nematode Bxadh2 gene significantly increased its expression in pine seedlings inoculated with endophytic Bacillus cereus GD2 and pine wood nematode. To explore pine wood nematode Bxadh2 gene function, we cloned and analyzed its biological information, and we preliminarily studied its physiological function through RNA interference. We found that the Bxadh2 gene’s full CDS length is 1269 bp, which encodes 422 amino acids, and presents a relatively stable hydrophobic protein. The protein encoded by the Bxadh2 gene has no signal peptide or transmembrane structure, and it is an intracellular protein that does not participate in transmembrane movement. The RNAi interference results showed that when the pine wood nematode’s Bxadh2 gene was suppressed, its survival rate and fecundity significantly decreased, indicating that the expression of the Bxadh2 gene was related to the growth and development of pine wood nematodes.
Inbreeding Effects on the Performance and Genomic Prediction for Polysomic Tetraploid Potato Offspring Grown at High Nordic Latitudes
Authors: Ortiz Rodomiro , Reslow Fredrik , Vetukuri Ramesh , García-Gil M. Rosario , Pérez-Rodríguez Paulino , Crossa José
Published: June 2023

Inbreeding depression (ID) is caused by increased homozygosity in the offspring after selfing. Although the self-compatible, highly heterozygous, tetrasomic polyploid potato (Solanum tuberosum L.) suffers from ID, some argue that the potential genetic gains from using inbred lines in a sexual propagation system of potato are too large to be ignored. The aim of this research was to assess the effects of inbreeding on potato offspring performance under a high latitude and the accuracy of the genomic prediction of breeding values (GEBVs) for further use in selection. Four inbred (S1) and two hybrid (F1) offspring and their parents (S0) were used in the experiment, with a field layout of an augmented design with the four S0 replicated in nine incomplete blocks comprising 100, four-plant plots at Umeå (63°49′30″ N 20°15′50″ E), Sweden. S0 was significantly (p < 0.01) better than both S1 and F1 offspring for tuber weight (total and according to five grading sizes), tuber shape and size uniformity, tuber eye depth and reducing sugars in the tuber flesh, while F1 was significantly (p < 0.01) better than S1 for all tuber weight and uniformity traits. Some F1 hybrid offspring (15–19%) had better total tuber yield than the best-performing parent. The GEBV accuracy ranged from −0.3928 to 0.4436. Overall, tuber shape uniformity had the highest GEBV accuracy, while tuber weight traits exhibited the lowest accuracy. The F1 full sib’s GEBV accuracy was higher, on average, than that of S1. Genomic prediction may facilitate eliminating undesired inbred or hybrid offspring for further use in the genetic betterment of potato.
Advancements in Focal Amplification Detection in Tumor/Liquid Biopsies and Emerging Clinical Applications
Authors: Arshadi Aram , Tolomeo Doron , Venuto Santina , Storlazzi Clelia
Published: June 2023

Focal amplifications (FAs) are crucial in cancer research due to their significant diagnostic, prognostic, and therapeutic implications. FAs manifest in various forms, such as episomes, double minute chromosomes, and homogeneously staining regions, arising through different mechanisms and mainly contributing to cancer cell heterogeneity, the leading cause of drug resistance in therapy. Numerous wet-lab, mainly FISH, PCR-based assays, next-generation sequencing, and bioinformatics approaches have been set up to detect FAs, unravel the internal structure of amplicons, assess their chromatin compaction status, and investigate the transcriptional landscape associated with their occurrence in cancer cells. Most of them are tailored for tumor samples, even at the single-cell level. Conversely, very limited approaches have been set up to detect FAs in liquid biopsies. This evidence suggests the need to improve these non-invasive investigations for early tumor detection, monitoring disease progression, and evaluating treatment response. Despite the potential therapeutic implications of FAs, such as, for example, the use of HER2-specific compounds for patients with ERBB2 amplification, challenges remain, including developing selective and effective FA-targeting agents and understanding the molecular mechanisms underlying FA maintenance and replication. This review details a state-of-the-art of FA investigation, with a particular focus on liquid biopsies and single-cell approaches in tumor samples, emphasizing their potential to revolutionize the future diagnosis, prognosis, and treatment of cancer patients.
Radiosensitizing Effects of Irinotecan versus Oxaliplatin Alone and in Combination with 5-Fluorouracil on Human Colorectal Cancer Cells
Authors: Frerker Bernd , Bock Felix , Cappel Marie-Louise , Kriesen Stephan , Klautke Gunther , Hildebrandt Guido , Manda Katrin
Published: June 2023

To date, oxaliplatin and irinotecan are used in combination with 5-flourouracil (5-FU) for metastatic colorectal cancer. In this study it was tested whether oxaliplatin and irinotecan and their combinations with 5-FU have an enhanced effect when treated simultaneously with ionizing radiation. In addition, it should be compared whether one combination therapy is more effective than the other. Colorectal cancer cells (HT-29) were treated with irinotecan or oxaliplatin, both alone and in combination with 5-FU, and subsequently irradiated. The cell growth, metabolic activity and proliferation of cells were investigated, and the clonogenic survival was determined. Furthermore, the assessment of radiation-induced DNA damage and the influence of the drugs and their combinations on DNA damage repair was investigated. Treatment with irinotecan or oxaliplatin in combination with 5-FU inhibited proliferation and metabolic activity as well as clonogenic survival and the DNA damage repair capacity of the tumor cells. The comparison of oxaliplatin and irinotecan with simultaneous irradiation showed the same effect of both drugs. When oxaliplatin or irinotecan was combined with 5-FU, tumor cell survival was significantly lower than with monotherapy; however, there was no superiority of either combination regimen. Our results have shown that the combination of 5-FU and irinotecan is as effective as the combination of 5-FU with oxaliplatin. Therefore, our data support the use of FOLFIRI as a radiosensitizer.
Impact of Manganese and Chromate on Specific DNA Double-Strand Break Repair Pathways
Authors: Haberland Vivien M. M. , Magin Simon , Iliakis George , Hartwig Andrea
Published: June 2023

Manganese is an essential trace element; nevertheless, on conditions of overload, it becomes toxic, with neurotoxicity being the main concern. Chromate is a well-known human carcinogen. The underlying mechanisms seem to be oxidative stress as well as direct DNA damage in the case of chromate, but also interactions with DNA repair systems in both cases. However, the impact of manganese and chromate on DNA double-strand break (DSB) repair pathways is largely unknown. In the present study, we examined the induction of DSB as well as the effect on specific DNA DSB repair mechanisms, namely homologous recombination (HR), non-homologous end joining (NHEJ), single strand annealing (SSA), and microhomology-mediated end joining (MMEJ). We applied DSB repair pathway-specific reporter cell lines, pulsed field gel electrophoresis as well as gene expression analysis, and investigated the binding of specific DNA repair proteins via immunoflourescence. While manganese did not seem to induce DNA DSB and had no impact on NHEJ and MMEJ, HR and SSA were inhibited. In the case of chromate, the induction of DSB was further supported. Regarding DSB repair, no inhibition was seen in the case of NHEJ and SSA, but HR was diminished and MMEJ was activated in a pronounced manner. The results indicate a specific inhibition of error-free HR by manganese and chromate, with a shift towards error-prone DSB repair mechanisms in both cases. These observations suggest the induction of genomic instability and may explain the microsatellite instability involved in chromate-induced carcinogenicity.
Genome-Wide Association Study of Lint Percentage in Gossypium hirsutum L. Races
Authors: Wang Yuanyuan , Guo Xinlei , Cai Xiaoyan , Xu Yanchao , Sun Runrun , Umer Muhammad Jawad , Wang Kunbo , Qin Tengfei , Hou Yuqing , Wang Yuhong , Zhang Pan , Wang Zihan , Liu Fang , Wang Qinglian , Zhou Zhongli
Published: June 2023

Lint percentage is one of the most essential yield components and an important economic index for cotton planting. Improving lint percentage is an effective way to achieve high-yield in cotton breeding worldwide, especially upland cotton (Gossypium hirsutum L.). However, the genetic basis controlling lint percentage has not yet been systematically understood. Here, we performed a genome-wide association mapping for lint percentage using a natural population consisting of 189 G. hirsutum accessions (188 accessions of G. hirsutum races and one cultivar TM-1). The results showed that 274 single-nucleotide polymorphisms (SNPs) significantly associated with lint percentage were detected, and they were distributed on 24 chromosomes. Forty-five SNPs were detected at least by two models or at least in two environments, and their 5 Mb up- and downstream regions included 584 makers related to lint percentage identified in previous studies. In total, 11 out of 45 SNPs were detected at least in two environments, and their 550 Kb up- and downstream region contained 335 genes. Through RNA sequencing, gene annotation, qRT-PCR, protein–protein interaction analysis, the cis-elements of the promotor region, and related miRNA prediction, Gh_D12G0934 and Gh_A08G0526 were selected as key candidate genes for fiber initiation and elongation, respectively. These excavated SNPs and candidate genes could supplement marker and gene information for deciphering the genetic basis of lint percentage and facilitate high-yield breeding programs of G. hirsutum ultimately.
Impact of Green Ceramic Hybrid Machining (GCHM) on Reliability and Repeatability of the Properties of Sintered Yttrium-Tetragonal Zirconia Polycrystal Parts
Authors: Ducobu François , Demarbaix Anthonin , Rivière-Lorphèvre Edouard , Spitaels Laurent , Petit Fabrice , Preux Nicolas , Duterte Charles , Mulliez Marylou , Lauwers Bert
Published: June 2023

The innovative Green Ceramic Hybrid Machining (GCHM) process sequentially combines milling with a cutting tool (GCM, Green Ceramic Machining) and laser beam machining (GCLBM) of a ceramic material (black Y-TZP in this study) at the green stage mainly to increase productivity, avoid taper angle limitations of laser beam machining, and obtain micro-features. The study focuses on the reliability and the repeatability of the properties of sintered parts obtained by three manufacturing processes (GCM, GCLBM, GCHM) to assess the performance of hybridisation. It turns out that GCHM is a compromise of both milling and laser beam processes; it increases the repeatability of the surface quality and it slightly reduces (less than 7%) the flexural strength by comparison to milling for a similar reliability. The study also highlights that the surface quality of GCLBM processed parts relies on of the surface generated by the previous operation. Milling that surface at the previous step is therefore recommended, corresponding to the sequence adopted by GCHM.