bluuebunny/crossref_metadata_2025 huggingface dataset:
Antenatal corticosteroids is associated with better postnatal growth outcomes of very preterm infants: A national multicenter cohort study in China
Authors: Li Tianhao , Shen Wei , Wu Fan , Mao Jian , Liu Ling , Chang Yanmei , Zhang Rong , Ye Xiuzhen , Qiu Yinping , Ma Li , Cheng Rui , Wu Hui , Chen Dongmei , Chen Ling , Xu Ping , Mei Hua , Wang Sannan , Xu Falin , Ju Rong , Zheng Zhi , Lin Xinzhu , Tong Xiaomei ,   The Chinese Multicenter EUGR Collaborative Group
Published: January 2023

Introduction

Antenatal corticosteroids (ACS) administration is a standardized prenatal care for accelerating fetal maturation before anticipated preterm delivery, however, its effect on nutrition and growth is yet uncertain. This study aimed to examine if ACS application is associated with improvement in postnatal growth and nutrition in very preterm infants (VPIs).

Methods

This was a secondary analysis of a multicenter prospective survey included infants born before 32 weeks gestation and admitted to 28 tertiary neonatal intensive care units throughout China from September 2019 to December 2020. Infants were divided into no ACS, partial ACS and complete ACS groups according to the steroids exposure. For infants exposed to antenatal corticosteroids, complete ACS was defined as receiving all doses of steroids 24 h-7 days before delivery, otherwise it was referred to partial ACS. The primary outcomes of postnatal growth were compared among the 3 groups. The multivariable regression analyses were applied to evaluate the association of different steroids coverage with postnatal growth and nutritional outcomes while adjusting for potential confounders. For each outcome, no ACS coverage was defined as the reference group. Data were presented as unstandardized coefficients or adjusted odds ratios with 95% confidence intervals, P < 0.05 (2-sided) indicated statistical significance.

Results

Among 2,514 infants included, complete ACS, partial ACS and no ACS group accounted for 48.7% (1,224/2,514), 29.2% (735/2,514) and 22.1% (555/2,514), respectively. The median weight growth velocity was 14.6 g/kg/d, 14.1 g/kg/d and 13.5 g/kg/d in complete, partial and no ACS group respectively with significant difference (P < 0.001). In multivariable analyses, both complete and partial ACS coverage were associated with shorter cumulative fasting time, faster weight growth velocity, less dramatic decline in Z-score of weight, and lower incidence of extrauterine growth restriction [aOR (95%CI): 0.603 (0.460, 0.789) and 0.636 (0.476,0.851), respectively] when compared with no ACS. Moreover, the faster length growth velocity and earlier enteral feeding start time were observed only in infants with complete ACS coverage.

Conclusions

Both complete and partial ACS are associated with better postnatal growth outcomes in very preterm infants. This efficacy appeared to be more obvious in infants exposed to complete ACS.
Case report: Chronic granulomatous disease presenting with early-onset inflammatory bowel disease and normal oxidative burst testing
Authors: Mondal Sanjib , Vignesh Pandiarajan , Loganathan Sathish Kumar , Arora Kanika , Das Jhumki , Rawat Amit , Singh Surjit
Published: January 2023

Background

Due to the lack of widespread availability of flow cytometry services for immunodeficiency, nitroblue tetrazolium test (NBT) is the commonly used screening modality to identify patients with chronic granulomatous disease (CGD) in developing countries.

Procedure

We report a child with X-linked CGD with residual NADPH oxidase activity who had an indeterminate NBT result even in the presence of classical manifestations of CGD.

Results

A 7-year-old boy presented with recurrent episodes of inflammatory colitis and Burkholderia cepacia septicaemia at the age of 3 years. He also had cervical adenitis due to Mycobacterium tuberculosis. NBT performed on multiple occasions was not suggestive of CGD. Dihydrorhodamine (DHR) test using phorbol myristate acetate (PMA) as a stimulant revealed a small blunt peak suggestive of AR-CGD; however, significant reduction in NADPH oxidase activity was noted with milder stimulants such as Escherichia coli and Staphylococcus aureus. Genetic analysis revealed a hemizygous pathogenic variant in CYBB. Flow cytometry showed diminished gp91phox expression in the patient's neutrophils suggestive of X-linked CGD.

Conclusion

Our case highlights that early-onset inflammatory bowel disease can be a presenting manifestation of CGD and diagnosis of CGD can be missed if NBT alone is used for screening, especially in the presence of NADPH oxidase activity. Diagnosis of “CGD with residual NADPH oxidase activity” requires a high degree of clinical suspicion, and performing DHR with different stimulants can unravel the diagnosis.
Haploinsufficiency of A20 in a Chinese child caused by loss-of-function mutations in TNFAIP3: A case report and review of the literature
Authors: Liu Jing , Lin Yuese , Li Xuandi , Ba Hongjun , He Xiufang , Peng Huimin , Li Shujuan , Zhu Ling
Published: January 2023

Case Presentation

A 3-year-and-6-month-old child was reported to have recurrent high fever with generalized lymph node enlargement and significant elevation of inflammatory markers such as C-reactive protein and procalcitonin in tests. Later, whole exome sequencing determined that the child's disease was haploinsufficiency of A20 (HA20).

Results

After immunosuppressive therapy, the child's symptoms improved significantly, and the inflammatory markers dropped to the normal range.

Conclusion

Because of the characteristics of HA20, this disease is often underdiagnosed and misdiagnosed in clinical practice. By reporting this case of HA20 in a child, we hope to increase the awareness of this disease in the clinic.
HIPPO signaling-related signature for predicting prognosis and therapeutic response in gastric cancer
Authors: Jiang Rui , Wang Jinghua , Liang Jun , Lin Daihua , Mao Qiuxian , Cheng Siyi , Huang Shengjun , Tong Shuangshuang , lyu Yanlin , Wei Rui , Lian Qizhou , Chen Hao
Published: January 2023

Background: Gastric cancer (GC) is a multifactorial progressive disease with high mortality and heterogeneous prognosis. Effective prognostic biomarkers for GC were critically needed. Hippo signaling pathway is one of the critical mechanisms regulating the occurrence and development of GC, and has potential clinical application value for the prognosis and treatment of GC patients. However, there is no effective signature based on Hippo signaling pathway-related genes (HSPRGs) to predict the prognosis and treatment response of GC patients. Our study aimed to build a HSPRGs signature and explore its performance in improving prognostic assessment and drug therapeutic response in GC.

Methods: Based on gene expression profiles obtained from The Cancer Genome Atlas (TCGA) database, we identified differentially expressed HSPRGs and conducted univariate and the least absolute shrinkage and selection operator (LASSO) Cox regression analysis to construct a multigene risk signature. Subsequently, the Kaplan-Meier curve and receiver operating characteristic (ROC) were performed to evaluate the predictive value of the risk signature in both training and validation cohort. Furthermore, we carried out univariate and multivariate Cox regression analysis to investigate the independent prognostic factors and establish a predictive nomogram. The enriched signaling pathways in risk signature were analyzed by gene set enrichment analysis (GSEA). Tumor immune dysfunction and exclusion (TIDE) and drug sensitivity analysis were performed to depict therapeutic response in GC.

Results: In total, 38 differentially expressed HSPRGs were identified, and final four genes (DLG3, TGFB3, TGFBR1, FZD6) were incorporated to build the signature. The ROC curve with average 1-, 3-, and 5-year areas under the curve (AUC) equal to .609, .634, and .639. Clinical ROC curve revealed that risk signature was superior to other clinicopathological factors in predicting prognosis. Calibration curves and C-index (.655) of nomogram showed excellent consistency. Besides, in the immunotherapy analysis, exclusion (p < 2.22 × 10–16) and microsatellite instability (p = .0058) performed significantly differences. Finally, our results suggested that patients in the high-risk group were more sensitive to specific chemotherapeutic agents.

Conclusion: Results support the hypothesis that Hippo-related signature is a novel prognostic biomarker and predictor, which could help optimize GC prognostic stratification and inform clinical medication decisions.
Integrated untargeted and targeted metabolomics to reveal therapeutic effect and mechanism of Alpiniae oxyphyllae fructus on Alzheimer’s disease in APP/PS1 mice
Authors: Zhou Shengnan , Liu Liwei , Zhang Yuanyuan , Zhang Zhibo , Li Hanbing , Fan Feng , He Jiuming , Kang Jian , Zuo Lihua
Published: January 2023

Introduction:Alpiniae oxyphyllae Fructus (AOF) has been abundantly utilized for the treatment of diarrhea, dyspepsia, kidney asthenia, and abdominal pain in China. AOF is effective for treating AD in clinical trials, but its exact mode of action is yet unknown.

Methods: In this study, metabolomics was combined to ascertain the alterations in plasma metabolism in APP/PS1 transgenic mice, the therapy of AOF on model mice, and the dynamic variations in 15 bile acids (BAs) concentration.

Results: 31 differential biomarkers were finally identified in APP/PS1 group vs. the WT group. The levels of 16 metabolites like sphinganine (Sa), lyso PE (20:2), lysoPC (17:0), glycocholic acid (GCA), deoxycholicacid (DCA) were increased in APP/PS1 group, and those of 15 metabolites like phytosphingosine, cer (d18:0/14:0), and fumaric acid were reduced in APP/PS1 group. After AOF treatment, 29 of the 31 differential metabolites showed a tendency to be back-regulated, and 15 metabolites were significantly back-regulated, including sphinganine (Sa), lyso PE (20:2), glycocholic acid (GCA), deoxycholic acid (DCA). The relationship between BAs level and AD had been received increasing attention in recent years, and we also found notable differences between DCA and GCA in different groups. Therefore, a BAs-targeted metabonomic way was established to determine the level of 15 bile acids in different groups. The consequence demonstrated that primary BAs (CA, CDCA) declined in APP/PS1 model mice. After 3 months of AOF administration, CA and CDCA levels showed an upward trend. Conjugated primary bile acids (TCA, GCA, TCDCA, GCDCA), and secondary bile acids (DCA, LCA, GDCA, TDCA, TLCA GLCA) ascended in APP/PS1 group. After 3 months of AOF treatment, the levels of most BAs decreased to varying degrees. Notably, the metabolic performance of DCA and GCA in different groups was consistent with the predictions of untargeted metabolomics, validating the correctness of untargeted metabolomics.

Discussion: According to metabolic pathways of regulated metabolites, it was prompted that AOF ameliorated the symptom of AD mice probably by regulating bile acids metabolism. This study offers a solid foundation for further research into the AOF mechanism for the therapy of AD.
Acute exercise mobilizes CD8+ cytotoxic T cells and NK cells in lymphoma patients
Authors: Koivula Tiia , Lempiäinen Salla , Rinne Petteri , Hollmén Maija , Sundberg Carl Johan , Rundqvist Helene , Minn Heikki , Heinonen Ilkka
Published: January 2023

Background: Studies have shown that acute exercise can mobilize several leukocyte subpopulations in healthy individuals. Our aim was to investigate whether a 10-min acute exercise has an effect on immune cell proportions in lymphoma patients.

Methods: This study included seven lymphoma patients referred to curative oncologic therapy. Three had Hodgkin and four non-Hodgkin lymphoma, one was female, and their mean age was 51. Patients underwent a 10-min acute exercise on a bicycle ergometer at moderate exercise intensity. Whole blood samples were taken at rest, immediately after exercise, and 30 min after exercise. Leukocyte subpopulation levels were determined using flow cytometry.

Results: Proportions of total NK cells and CD56+CD16+ NK cells of total leukocytes increased immediately after exercise and decreased back to baseline at 30 min post-exercise. Proportion of CD8+ T cells of total T cells increased and proportion of CD4+ T cells of total T cells decreased immediately after exercise, and both returned to baseline at 30 min post-exercise. There was no change in the proportions of B cells, granulocytes, or monocytes. Exercising diastolic blood pressure correlated positively with changes in total NK cell and CD56+CD16+ NK cell proportions, and exercising mean arterial pressure correlated positively with change in CD56+CD16+ NK cell proportion.

Conclusion: Our findings indicate that a single acute exercise bout of only 10 min can cause leukocytosis in lymphoma patients, particularly on cytotoxic T cells and NK cells, which are the most important immune cells fighting against cancer.
Real-time determination of flowering period for field wheat based on improved YOLOv5s model
Authors: Song Xubin , Liu Lipeng , Wang Chunying , Zhang Wanteng , Li Yang , Zhu Junke , Liu Ping , Li Xiang
Published: January 2023

The flowering period is one of the important indexes of wheat breeding. The early or late flowering affects the final yield and character stability of wheat. In order to solve the problem that it is difficult to accurately and quickly detect the flowering period of a large number of wheat breeding materials, a determination method of flowering period for field wheat based on the improved You Only Look Once (YOLO) v5s model was proposed. Firstly, a feature fusion (FF) method combing RGB images and corresponding comprehensive color features was proposed to highlight more texture features and reduce the distortion caused by light on the extracted feature images. Second, the YOLOv5s model was selected as a base version of the improved model and the convolutional block attention model (CBAM) was adopted into the feature fusion layer of YOLOV5s model. Florets and spikelets were given greater weight along the channel and spatial dimensions to further refine their effective feature information. At the same time, an integrated Transformer small-target detection head (TSDH) was added to solve the high miss rate of small targets in wheat population images. The accurate and rapid detection of florets and spikelets was realized, and the flowering period was determined according to the proportion of florets and spikelets. The experimental results showed that the average computing time of the proposed method was 11.5ms, and the average recognition accuracy of florets and spikelets was 88.9% and 96.8%, respectively. The average difference between the estimated flowering rate and the actual flowering rate was within 5%, and the determination accuracy of the flowering period reached 100%, which met the basic requirements of the flowering period determination of wheat population in the field.
Transcriptomic and proteomic analyses provide insights into host adaptation of a bamboo-feeding aphid
Authors: Zhang Hui , Lin Ruixun , Liu Qian , Lu Jianjun , Qiao Gexia , Huang Xiaolei
Published: January 2023

Introduction

Salivary glands and their secreted proteins play an important role in the feeding process of sap-sucking aphids. The determination of saliva composition is an important step in understanding host plant adaptation of aphids. Pseudoregma bambucicola is a severe bamboo pest in subtropical areas and the only aphid species that can exclusively feed on hard stalks of bamboos. How this species can penetrate and degrade hard bamboo cell walls and utilize a very specialized niche are important unanswered questions.

Methods

In this study, comprehensive analyses based on transcriptome sequencing, RT-qPCR, liquid chromatography-tandem spectrometry (LC–MS/MS) and bioinformatics were conducted on dissected salivary glands and secreted saliva of P. bambucicola to characterize the overall gene expression and salivary protein composition, and to identify putative effector proteins important for aphid-plant interactions.

Results and Discussion

Some secretory proteins homologous to known aphid effectors important for aphid–plant interactions, such as digestive enzymes, detoxifying and antioxidant enzymes and some effectors modulating plant defenses, are also detected in salivary gland transcriptome and salivary gland and/or saliva secretomes in P. bambucicola. This indicates that these effectors are probably be essential for enabling P. bambucicola feeding on bamboo host. Although several plant cell wall degrading enzymes (PCWDEs) can be identified from transcriptome, most of the enzymes identified in salivary glands showed low expression levels and they only represent a small fraction of the complete set of enzymes for degrading cellulose and hemicellulose. In addition, our data show that P. bambucicola has no its own ability to produce pectinases. Overall, our analyses indicate that P. bambucicola may lose its own ability to express and secrete key PCWDEs, and its adaptation to unique feeding habit may depend on its symbiotic bacteria.
Deepening apartheid: The political geography of colonizing Israel/Palestine
Authors: Yiftachel Oren
Published: January 2023

The paper analyzes the regime in Israel/Palestine using a political geographical perspective. It demonstrates how a combination of colonial, national, capitalist and liberal forces have put in train a process of “deepening apartheid” in the entire territory controlled by Israel—between River and Sea. This undeclared regime has been established to guard the 'achievements' of settler colonial Judaization of the land and the domination of the Jewish minority. As described by the Rome Statute, it has become an institutionalized regime of systematic oppression and domination by one group over others, with the intention of maintaining that regime. Hence the political geographical analysis shows clearly that the wide description in academic and international circles of Israel as “Jewish and democratic,” is based on a denial of the clear racialized hierarchy of civil statuses. This setting enhances Jewish supremacy (using different methods) it in all regions, while Palestinians are fragmented into lower rungs on the ethnic hierarchy—de facto and de jure—thereby contradicting the tenets of democratic civil equality. Theoretically, the paper draws on the links between settler colonial expansion, the rise of ethnocracy, partial liberalization under global capitalism, and the making of apartheid. It shows that historically Jewish colonization of Palestine—the underlying logic of the regime—has advanced in six main historical-geographical stages, encountering persistent, and at times violent, Palestinian resistance. The paper then analyzes in more detail the emergence of one regime between River and Sea, in which the state uses military, spatial, economic, and legal powers, as well as geopolitical maneuvering (particularly US support) to oppress Palestinians, while promoting democratic rights and economic development among Jews. This has enabled Israel to integrate and “whiten” Mizrahi and other Jewish groups into mainstream Zionism. Rivaling Palestinian political projects have been fragmented, ghettoized, attacked violently, and severely weakened. The paper shows how Jewish colonization, on both sides of the Green Line (which has also included some tactical withdrawals), has led to the establishment of four hierarchical types of citizenship, governed as “separate and unequal”. The relations between the groups resemble the racialized categories used in Apartheid South Africa and include (a) “White”—Jewish Israelis—with full citizenship rights; (b) “Colored”—Palestinian Arabs with Israeli citizenship with partial rights; (c) “Black”—Palestinians under occupation lacking citizenship or political rights; (d) “Gray”—an emerging group, consisting of non-citizen migrants and asylum seekers.
Influences of age-related positivity effect on characteristics of odor-evoked autobiographical memories in older Japanese adults
Authors: Yamamoto Kohsuke , Sugiyama Haruko
Published: January 2023

Older adults tend to remember past life events more positively than younger adults. This tendency is the age-related positivity effect. The present study examined whether this effect occurred for odor-evoked autobiographical memories. In total, 317 young and 181 older Japanese participants were asked to recall autobiographical events evoked by odors. Participants then completed the odor-evoked autobiographical memory questionnaire (OEAMQ) to measure the characteristics of the recalled memories. In the results, older participants recalled more positive memories than younger participants. Older participants also rated the OEAMQ subscales higher than the younger participants. Furthermore, there were significant positive correlations between the ratings of odor emotional characteristics and OEAMQ subscales. The age-related positivity effect was observed for odor-evoked autobiographical memories. The emotion aroused by odor played a significant role in some attributes of odor-evoked autobiographical memory. The age-related positivity effect in odor-evoked autobiographical memories has important implications for understanding the cognitive mechanisms of aging in autobiographical memory and olfaction and for applications in well-being and dementia in older adults.
Modeling the relationship between digital nativity and Smartphone usage in learning English as a foreign language contexts
Authors: Hui Lianghong , Teng Lin Sophie , Guo Fangfang
Published: January 2023

Introduction

Despite many studies exploring the application of digital devices in foreign language learning, only some have investigated the influencing mechanisms of digital nativity on Smartphone usage in this increasingly seamless learning environment. This research aims to explore the relationships between college students’ digital nativity and their use of Smartphones for English learning.

Methods

The data were collected from 502 undergraduates in mainland China through self-reported questionnaires, namely the Digital Natives Assessment Scale and the Smartphone Use in Learning Foreign Language Scale.

Results and Discussion

The confirmatory factor analysis validated a four-factor measurement model of digital nativity, including “grow up with technology”, “comfortable with multitasking”, “reliant on graphics for communication” and “thrive on instant gratification and rewards”. A second-order measurement construct of favorable Smartphone usage and its first-order adverse effects in foreign language learning were also examined, demonstrating good validity and reliability. Structural equation modeling analysis revealed that students who displayed more attributes of “grow up with technology” and “thrive on instant gratifications and rewards” tended to adopt smartphones positively for English learning. In addition, those who were more familiar with technological assistance might suffer less from the adverse effects of Smartphone usage. However, the preference for immediate responses and feedback could also lead to more adverse effects when using Smartphones for English learning. Besides, “comfortable with multitasking” and “reliant on graphics for communication” didn’t have any significant predictive impact on either the favorable functions or the adverse effects of Smartphone usage. Based on the research results, we discuss the theoretical and practical implications.
Psychometric evaluation of the Chinese version of fear of hospitalization scale among outpatients: A validation study
Authors: Li Wenbo , Yu Hongyu , Zhang Yanli , Li Bing , Fu Mingshu
Published: January 2023

Background

In China, some patients avoid seeking medical care and are highly sensitive to subsequent medical care because of fear of possible hospitalization after a diagnosis has been established. Early identification of fear of hospitalization is essential for clinical staff to develop targeted education and interventions. However, there are currently no tools to assess outpatients’ fear of hospitalization in mainland China. This study aimed to translate the Fear of Hospitalization (FH) scale into Chinese and verify its reliability and validity in outpatients.

Methods

Through convenience sampling, 664 outpatients who required hospitalization were recruited from two cities in Liaoning Province, China. The reliability of the translated scale was measured by internal consistency, split-half reliability, and test–retest reliability. The validity of the translated scale was evaluated by expert consultation, exploratory factor analysis, and confirmatory factor analysis. Data were analyzed using SPSS 25.0 (IBM Corp., Armonk, NY, United States) and AMOS 23.0 (IBM Corp., Armonk, NY, United States).

Results

The Cronbach’s α value of the Chinese version of the FH scale was 0.849, and the Cronbach’s α value of the dimensions ranged from 0.857 to 0.902. The test–retest reliability value of 0.868 shows good temporal stability. The split-half reliability value of 0.910 indicates a high degree of measuring the same content. The content validity index of the scale (S-CVI) was 0.924, indicating a good level of content validity. The 3-factor structure supported by eigenvalues, total variance explained, and scree plot was obtained using exploratory factor analysis. In addition, all recommended fit indicators were within the acceptable range by confirmatory factor analysis.

Conclusion

The Chinese version of the FH scale is valid and reliable in outpatients. The developed three-factor structured scale will help identify outpatients with a high fear of hospitalization and can inform the development of educational intervention plans for care managers, physicians, and nurses. In addition, it helps clinicians and nurses take action to reduce this fear of hospitalization in patients and prevent avoidance of using health care services due to fear of hospitalization.
DMFF-Net: A dual encoding multiscale feature fusion network for ovarian tumor segmentation
Authors: Wang Min , Zhou Gaoxi , Wang Xun , Wang Lei , Wu Zhichao
Published: January 2023

Ovarian cancer is a serious threat to the female reproductive system. Precise segmentation of the tumor area helps the doctors to further diagnose the disease. Automatic segmentation techniques for abstracting high-quality features from images through autonomous learning of model have become a hot research topic nowadays. However, the existing methods still have the problem of poor segmentation of ovarian tumor details. To cope with this problem, a dual encoding based multiscale feature fusion network (DMFF-Net) is proposed for ovarian tumor segmentation. Firstly, a dual encoding method is proposed to extract diverse features. These two encoding paths are composed of residual blocks and single dense aggregation blocks, respectively. Secondly, a multiscale feature fusion block is proposed to generate more advanced features. This block constructs feature fusion between two encoding paths to alleviate the feature loss during deep extraction and further increase the information content of the features. Finally, coordinate attention is added to the decoding stage after the feature concatenation, which enables the decoding stage to capture the valid information accurately. The test results show that the proposed method outperforms existing medical image segmentation algorithms for segmenting lesion details. Moreover, the proposed method also performs well in two other segmentation tasks.
Inverted U-shaped relationship between education and family health: The urban-rural gap in Chinese dual society
Authors: Jia Changli , Long Yanwen , Luo Xiaoxia , Li Xiao , Zuo Wenjing , Wu Yibo
Published: January 2023

Introduction

The Healthy China Initiative emphasizes family health. Education is an upstream determinant of health, which can both achieve upward mobility and cause class solidification.

Methods

Using nationwide large-scale data collected in 2021, the present study explored the relationship between education and family health in the urban-rural dual society via Oaxaca-Blinder decomposition and propensity score matching.

Results

Our data revealed disparities in family health, educational attainment, household income, healthcare coverage, and job type between urban and rural China. An inverted U-shaped relationship existed between increasing years of education and family health. The upper limit was 17.1 years for urban residents and 13.7 years for rural residents, with limited health benefits from higher education obtained by rural residents. Mediated by work-family conflict, highly-educated people received gradually diminishing health returns. The results of the Oaxaca-Blinder decomposition showed that 25.8% of the urban-rural gap in family health could be explained by the disparity in education. Urban residents could translate cultural capital and economic capital into health capital to a greater extent. After propensity score matching, a robust, inverted U-shaped relationship was found between education and family health. The inverted U-shaped relationship was found to replace family health with self-rated health and quality of life.

Discussion

Family-centered public health and education programs, policies, and goals should be developed to break urban-rural dual structure barriers and advance social equity in China.
Epidemiological characteristics of leprosy during the period 2005–2020: A retrospective study based on the Chinese surveillance system
Authors: Li Xiang , Jin Guangjie , Yang Jing , Li Yunhui , Wei Pingmin , Zhang Lianhua
Published: January 2023

Background

Jiangsu Province is located in the Yangtze River Delta region, with a total area of 107,200 square kilometers. Since 1949, over 55,000 cases have been registered, with Taixing accounting for the highest number of patients. The proportion of new cases with MB and G2D was higher compared to other regions. As a result, Jiangsu has been considered a priority area for public health interventions in China.

Methods

This paper mainly described the population, time, and spatial distribution of the newly detected leprosy cases in Jiangsu Province between 2005 and 2020. In this study, all the data were entered into Microsoft Excel and SPSS for the descriptive analysis. ArcGIS was applied to create statistical maps, and Geoda was used to conduct spatial autocorrelation analysis with local Moran's I statistics (LISA). The epidemiological data were obtained from LEPMIS. In addition, population data were obtained from the Statistical Yearbook of Jiangsu Province.

Results

During the study period, 363 new cases were reported. Of these, 232 were men and 131 were women (1.77:1). The mean age at diagnosis was 60.56 years, and no adolescent cases were identified. Three hundred and twenty-seven (90.08%) were diagnosed with MB and 36 (9.92%) with PB. 31.68% (115/363) of the patients presented with G2D. Farmers accounted for 74.9%, and most cases were identified in skin clinics (248, 68.32%). We observed a decreasing trend in detection rate, with a higher concentration of new cases diagnosed between July and October. Spatial analysis showed that the new cases were primarily distributed in the northwest of Jiangsu province, and Suqian has the highest incidence of leprosy. Special attention should be paid to Wuzhong, a county with a potential risk of inter-provincial transmission. Furthermore, 55 new cases came from other Chinese provinces but lived in Jiangsu.

Conclusion

The NCDR of leprosy decreased, but the new cases showed disabilities, a sign of the late diagnosis. The results indicated that some regions were still suffering from the burden of leprosy. Thus, we recommend that the government should adopt effective strategies to promote leprosy control. The main priorities for eliminating new cases were to provide sustainable financial support, improve the quality of clinical services, strengthen preventive intervention and rehabilitation services for disabilities, provide health education among high-risk populations, and explore new approaches.
Automatic classification of signal regions in 1H Nuclear Magnetic Resonance spectra
Authors: Fischetti Giulia , Schmid Nicolas , Bruderer Simon , Caldarelli Guido , Scarso Alessandro , Henrici Andreas , Wilhelm Dirk
Published: January 2023

The identification and characterization of signal regions in Nuclear Magnetic Resonance (NMR) spectra is a challenging but crucial phase in the analysis and determination of complex chemical compounds. Here, we present a novel supervised deep learning approach to perform automatic detection and classification of multiplets in 1H NMR spectra. Our deep neural network was trained on a large number of synthetic spectra, with complete control over the features represented in the samples. We show that our model can detect signal regions effectively and minimize classification errors between different types of resonance patterns. We demonstrate that the network generalizes remarkably well on real experimental 1H NMR spectra.
Citizen science initiatives increase pollinator activity in private gardens and green spaces
Authors: Persson Anna S. , Hederström Veronica , Ljungkvist Iris , Nilsson Lovisa , Kendall Liam
Published: January 2023

Wild insect pollinators are essential to cultivated and natural ecosystems globally. Today, many pollinator species are declining. One reason is a general lack of flowering habitats at landscape scales. However, urban areas, including private gardens, may provide flowers, and constitute beneficial habitats for pollinators. Here, we evaluate the ecological outcomes of a citizen science campaign run by the Swedish Society for Nature Conservation (SSNC) (called “Operation: Save the bees”), encouraging citizens to incorporate interventions beneficial to wild pollinators (garden meadows, flower plantings, and bee hotels) in their gardens. Data on insect observations and flowering plants were collected through online questionnaires at the end of the growing season. In total, we received 3,758 responses for the three interventions. We found that participants were more likely to observe many pollinators (as opposed to few or none) in more species rich garden meadows, and in larger and older plantings. The surrounding environment also affected pollinator abundance: fewer pollinators were observed in plantings in dense urban areas. Direct counts of pollinators during 10-min surveys correlated strongly to the simplistic abundance assessment (none, few, or many insects seen over the summer season). Bee hotel occupancy was positively related to local flower availability and bee hotel age. Smaller nest holes (<10 mm) were more occupied than larger holes (11–15 mm) and hotels in rural gardens and natural/semi-natural sites were more occupied than those in urban gardens. This study demonstrates that flower-rich private gardens provide integral habitat for wild pollinators and that citizen science programs can provide a tool for implementing and evaluating conservation practices. However, longer lasting commitment resulting in older interventions are preferable and should be encouraged in future campaigns.
Seeing the faces is so important—Experiences from online team meetings on commercial virtual reality platforms
Authors: Bonfert Michael , Reinschluessel Anke V. , Putze Susanne , Lai Yenchin , Alexandrovsky Dmitry , Malaka Rainer , Döring Tanja
Published: January 2023

During the COVID-19 pandemic, online meetings became common for daily teamwork in the home office. To understand the opportunities and challenges of meeting in virtual reality (VR) compared to videoconferences, we conducted the weekly team meetings of our human-computer interaction research lab on five off-the-shelf online meeting platforms over 4 months. After each of the 12 meetings, we asked the participants (N = 32) to share their experiences, resulting in 200 completed online questionnaires. We evaluated the ratings of the overall meeting experience and conducted an exploratory factor analysis of the quantitative data to compare VR meetings and video calls in terms of meeting involvement and co-presence. In addition, a thematic analysis of the qualitative data revealed genuine insights covering five themes: spatial aspects, meeting atmosphere, expression of emotions, meeting productivity, and user needs. We reflect on our findings gained under authentic working conditions, derive lessons learned for running successful team meetings in VR supporting different kinds of meeting formats, and discuss the team’s long-term platform choice.
Cutting edge concepts: Does bilirubin enhance exercise performance?
Authors: Flack Kyle D. , Vítek Libor , Fry Christopher S. , Stec David E. , Hinds Terry D.
Published: January 2023

Exercise performance is dependent on many factors, such as muscular strength and endurance, cardiovascular capacity, liver health, and metabolic flexibility. Recent studies show that plasma levels of bilirubin, which has classically been viewed as a liver dysfunction biomarker, are elevated by exercise training and that elite athletes may have significantly higher levels. Other studies have shown higher plasma bilirubin levels in athletes and active individuals compared to general, sedentary populations. The reason for these adaptions is unclear, but it could be related to bilirubin's antioxidant properties in response to a large number of reactive oxygen species (ROS) that originates from mitochondria during exercise. However, the mechanisms of these are unknown. Current research has re-defined bilirubin as a metabolic hormone that interacts with nuclear receptors to drive gene transcription, which reduces body weight. Bilirubin has been shown to reduce adiposity and improve the cardiovascular system, which might be related to the adaption of bilirubin increasing during exercise. No studies have directly tested if elevating bilirubin levels can influence athletic performance. However, based on the mechanisms proposed in the present review, this seems plausible and an area to consider for future studies. Here, we discuss the importance of bilirubin and exercise and how the combination might improve metabolic health outcomes and possibly athletic performance.
Successful management of a rare case of juvenile giant right ventricular myxoma
Authors: Gong Ke , Yang Yifeng , Shen Yadan , Liu Haidan , Xie Li , Liu Jijia
Published: January 2023

Primary cardiac tumors are extremely uncommon in young children and infants. Cardiac myxoma are typically found in the atria, predominately in the left atrium, with relatively few found on the right side, such as in the right ventricle or pulmonary artery. Numerous significant complications, including sudden death, can result from obstruction of the main pulmonary artery trunk and right ventricular outflow tract. Here, we describe the case of a 14-year-old Chinese girl diagnosed with a right ventricular myxoma located in the right ventricle and extended into the main pulmonary trunk. Complete resection of the myxoma and histological confirmation were performed.
C-reactive protein as a tool for monitoring response to treatment in dogs with acute hemorrhagic diarrhea syndrome
Authors: Sänger Florian , Unterer Stefan , Werner Melanie , Dörfelt René
Published: January 2023

Objectives

C-reactive protein (CRP) is an established marker for systemic inflammation in dogs that is especially elevated in dogs with sepsis. Some dogs with acute hemorrhagic diarrhea syndrome (AHDS) develop bacterial translocation and consequent sepsis during hospitalization. This study aimed to evaluate the course of CRP plasma concentrations during hospitalization and its correlation with clinical and other laboratory variables in dogs with AHDS.

Methods

In this prospective, observational study, CRP was evaluated on days 0, 1, 2, and 3 in 27 client-owned dogs who presented with AHDS. Clinical examination data, blood pressure, acute patient physiologic and laboratory evaluation (APPLE) full and APPLE fast scores, and canine hemorrhagic diarrhea severity (CHDS) index were measured on the same days to evaluate the severity of the disease.

Results

Twenty-five of the 27 dogs were discharged from hospital. Nineteen dogs received antimicrobial treatment due to sepsis or neutropenia. CRP values were mildly elevated on day 0 (median 27.3 mg/L; 1.0–125.8 mg/L) and markedly elevated on day 1 (median 88.9 mg/L; 1.4–192.7 mg/L). CRP concentrations decreased gradually over the following days. Moreover, CRP concentrations correlated moderately with albumin, leucocyte count, neutrophil count, and APPLE full and fast scores, but not with antimicrobial treatment.

Conclusion and relevance

CRP concentrations were significantly elevated in patients with AHDS. In this study population, CRP did not help in detecting the requirement of antimicrobial treatment in dogs with AHDS. Nevertheless, as CRP can monitor the response to treatment, regular analysis can guide treatment.
Microencapsulated phage composites with increased gastrointestinal stability for the oral treatment of Salmonella colonization in chicken
Authors: Zhang Bo , Wang Yongxia , Wang Fangfang , Zhang Yongying , Hao He , Lv Xingbang , Hao Liuhang , Shi Yuxiang
Published: January 2023

Salmonella infection, one of the common epidemics in the livestock and poultry breeding industry, causes great economic losses worldwide. At present, antibiotics are the most commonly used treatment for Salmonella infection, but the widespread use of antibiotics has increased drug resistance to Salmonella. Phage therapy has gradually become an alternative method to control Salmonella infection. However, phage, a specific virus that can infect bacteria, has poor stability and is prone to inactivation during treatment. Microencapsulated phage microspheres can effectively solve this problem. Accordingly, in this study, Salmonella phages were microencapsulated, using the xanthan gum/sodium alginate/CaCl2/chitooligosaccharides method, to improve their gastrointestinal stability. Furthermore, microencapsulated phages were evaluated for in vitro temperature and storage stability and in vivo therapeutic effect. Phage microspheres prepared with 1 g/100 mL xanthan gum, 2 g/100 mL sodium alginate, 2 g/100 mL CaCl2, and 0.6 g/100 mL chitooligosaccharides were regular in shape and stable in the temperature range of 10–30°C. Also, microencapsulated phages showed significantly improved stability in the simulated gastric juice environment than the free phages (p < 0.05). In the simulated intestinal fluid, microencapsulated phages were completely released after 4 h. Moreover, microencapsulated phages showed good storage stability at 4°C. In the in vivo experiments detecting Salmonella colonization in the intestinal tract of chicks, microencapsulated phages showed a better therapeutic effect than the free phages. In conclusion, microencapsulated phages exhibited significantly improved stability, gastric acid resistance, and thereby efficacy than the free phages. Microencapsulated phages can be potentially used as biological control agents against bacterial infections.
Initial investigation of molecular phenotypes of airway mast cells and cytokine profiles in equine asthma
Authors: Woodrow Jane S. , Hines Melissa , Sommardahl Carla , Flatland Bente , Lo Yancy , Wang Zhiping , Sheats Mary Katie , Lennon Elizabeth M.
Published: January 2023

Equine asthma is a naturally occurring lung disease characterized by chronic, partially reversible airway obstruction, pulmonary remodeling, and lower airway inflammation. Asthma is currently divided into two major groups, mild to moderate asthma (mEA) and severe asthma (sEA), but further subtyping by phenotype (i.e., clinical presentation) and/or endotype (i.e., cellular mechanisms) may be warranted. For this study, we were interested in further investigation of cellular and inflammatory characteristics of EA, including airway mast cells. The purpose of this study was to: (1) compare mast cell protease mRNA expression between healthy and asthmatic horses, (2) analyze the cytokine profile present in BALF of currently defined equine asthma groups, and (3) use these data to evaluate potential biomarkers of defined asthma groups. We hypothesized that there would be significant differences in the cellular mast cell phenotypes (i.e., mucosal vs. connective tissue) and cytokine profiles in the BALF of asthmatic vs. healthy horses and across asthma groups. We assert these characteristics may inform additional subtypes of equine asthma. Adult horses were recruited from the institution's teaching herd and clinical caseload. Mast cell protease gene expression of the BALF cellular component and multiplex bead immunoassay for cytokine concentrations in the BALF supernatant were investigated. Airway mast cells primarily expressed tryptase, with low levels of chymase. No significant changes in protease expression were detected across groups. Horses with severe asthma had increased TNF-α, CXCL-8, and IFN-γ concentrations in BALF supernatant. Multidimensional analysis demonstrated healthy and mEA horses have overlapping characteristics, with sEA separating from the other groups. This difference was primarily due to BALF neutrophil and lymphocyte concentrations. These study results further inform understanding of EA immunopathology, and future studies designed to investigate asthma phenotypes and endotypes. Ultimately, a better understanding of these groups could help identify novel therapeutic strategies.
The Importance of Modeling Path Choice Behavior in the Vehicle Routing Problem
Authors: Vitetta Antonino
Published: January 2023

Given two pick-up and delivery points, the best path chosen does not necessarily follow the criteria of minimum travel time or generalized minimum cost evaluated with a deterministic approach. Given a criterion, the perceived cost is not deterministic for many reasons (congestion, incomplete information on the state of the system, inexact prediction of the system state, etc.). The same consideration applies to the best-chosen route, assuming that the route is an ordered list of network nodes to visit. The paths and routes perceived and chosen (drivers or companies) could follow different criteria (i.e., minizmum congested travel time for the path and minimum monetary cost for the route). In this context, the paths chosen between two pick-up and delivery points, studied with the path choice problem (PCP), influence the best route, studied with the vehicle routing problem (VRP). This paper reports some considerations on the importance of modelling the path choice behavior in the VRP; the influence of the PCP on the VRP is studied. The considerations are supported by a numerical example in a small network in which the results obtained by adopting the deterministic or probabilistic models for the PCP are compared. To validate the reported thesis, the models are applied in a small test system, and it allows the reader to follow the numerical results step by step.
Convex Optimization-Based Techniques for Trajectory Design and Control of Nonlinear Systems with Polytopic Range
Authors: Jansson Olli , Harris Matthew W.
Published: January 2023

This paper presents new techniques for the trajectory design and control of nonlinear dynamical systems. The technique uses a convex polytope to bound the range of the nonlinear function and associates with each vertex an auxiliary linear system. Provided controls associated with the linear systems can be generated to satisfy an ordering constraint, the nonlinear control is computable by the interpolation of controls obtained by convex optimization. This theoretical result leads to two numerical approaches for solving the nonlinear constrained problem: one requires solving a single convex optimization problem and the other requires solving a sequence of convex optimization problems. The approaches are applied to two practical problems in aerospace engineering: a constrained relative orbital motion problem and an attitude control problem. The solve times for both problems and approaches are on the order of seconds. It is concluded that these techniques are rigorous and of practical use in solving nonlinear trajectory design and control problems.