bluuebunny/crossref_metadata_2025 huggingface dataset:
Extraction Study of Lignite Coalbed Methane as a Potential Supplement to Natural Gas for Enhancing Energy Security of Western Macedonia Region in Greece
Authors: Vasiliadou Ioanna A. , Semizoglou Zacharoula A. , Karayannis Vayos G. , Tsanaktsidis Constantinos G.
Published: December 2023

Greek lignite reserves are mainly located in the northwestern part of the country (Region of Western Macedonia, Greece), reaching a total of 5 billion tons. Considering that Greece is planning to stop burning lignite for electricity production, the recovery of the CH4 trapped in lignite coalbed reservoirs can be a valuable alternative for power generation and may help to reduce the direct emissions of methane during mining activities. The aim of the present study was to evaluate the LCBM in the Region of Western Macedonia, Greece (Ptolemaida basin). In order to assess the LCBM that could be extracted, three samples were collected from an active mine and were subjected to desorption experiments at different temperatures (25 °C, 50 °C, 100 °C, and 150 °C) by channeling high purity Ar gas at 1 atm of pressure. According to the results, the highest amount of CH4 was extracted during the desorption process at 50 °C, while the total amount of CH4 from all three samples was 0.82 m3/kg, confirming the presence of CH4 in the lignite deposits. Finally, a SWOT analysis was carried out that shows the strengths and opportunities against the weaknesses and threats of a potential LCBM exploitation in Greece, while also taking into account the social, economic, and environmental nexus.
Development and Testing of an Active Noise Control System for Urban Road Traffic Noise
Authors: Yang Biyu , Yin Jiacun , Ye Zhoujing , Yang Songli , Wang Linbing
Published: December 2023

As urbanization accelerates, the increasing number of vehicles and travel demands contribute to escalating road traffic noise pollution. Although passive noise control techniques such as noise barriers and green belts effectively mitigate noise, they occupy urban space, exacerbating the scarcity and high cost of already congested city areas. Emerging as a novel noise reduction strategy, active noise control (ANC) eliminates the need for physical isolation structures and addresses the noise within specific frequency ranges more effectively. This paper investigates the characteristics of urban road traffic noise and develops an ANC prototype. Utilizing the Least Mean Squares (LMS) algorithm, we conduct active noise control tests for various types of single- and dual-frequency noise within the prototype’s universal platform to validate its actual noise reduction capabilities. The study demonstrates that urban road traffic noise is mostly in the mid- to low-frequency range (below 2000 Hz). The developed ANC prototype significantly reduces single- or dual-frequency noise within this range, achieving a maximum noise reduction of nearly 30 dB(A). Future research should expand noise reduction tests across more frequency bands and assess the noise reduction effectiveness against real road traffic noise.
Novel Materials as Exogenous Carbon Sources for Denitrifying Biofilters
Authors: Kłobukowska Karolina , Rodziewicz Joanna , Mielcarek Artur , Bryszewski Kamil Łukasz , Janczukowicz Wojciech , Bugajski Piotr , Jóźwiakowski Krzysztof , Operacz Agnieszka
Published: December 2023

The problem of wastewater discharged from facilities for soilless plant cultivation is a worldwide problem. So, there is a strong need to find a simple, maintenance-free technological solution. Such a solution may be the use of biofilters or constructed wetlands with “active” filling, which will be a source of carbon for denitrifying organisms. The aim of this study was to evaluate four different types of materials—Oxytree wood chips (Paulownia Clon in vitro 112®), biobased polybutylene succinate derivatives (BioPBS FZ91PB and BioPBS FD92PB), and beech wood chips—as biofilter media and as solid exogenous carbon sources. The highest efficiency of denitrification and dephosphatation (43.11% and 42.48%, respectively) was characterized by the biobased polybutylene succinate FD92PB. The lowest removal efficiency of phosphorus compounds was achieved for beech chips—25.23% and BioPBS FZ91PB—26.42%. The lowest denitrification efficiency—8.8%—was achieved by Oxytree wood chips. The extent of organic matter (COD) repollution in the treated wastewater requires further study. Wood chips were found to release less COD than biobased polybutylene succinate. The research results showed that all tested materials can be a source of carbon in soilless tomato cultivation wastewater treatment. This is a good starting point for further research on selecting appropriate process parameters and creating new solutions for reactor construction.
An Enhanced Dual-Stream Network Using Multi-Source Remote Sensing Imagery for Water Body Segmentation
Authors: Zhang Xiaoyong , Geng Miaomiao , Yang Xuan , Li Cong
Published: December 2023

Accurate surface water mapping is crucial for rationalizing water resource utilization and maintaining ecosystem sustainability. However, the diverse shapes and scales of water bodies pose challenges in automatically extracting them from remote sensing images. Existing methods suffer from inaccurate lake boundary extraction, inconsistent results, and failure to detect small rivers. In this study, we propose a dual-stream parallel feature aggregation network to address these limitations. Our network effectively combines global information interaction from the Swin Transformer network with deep local information integration from Convolutional Neural Networks (CNNs). Moreover, we introduce a deformable convolution-based attention mechanism module (D-CBAM) that adaptively adjusts receptive field size and shape, highlights important channels in feature maps automatically, and enhances the expressive ability of our network. Additionally, we incorporate a Feature Pyramid Attention (FPA) module during the advanced coding stage for multi-scale feature learning to improve segmentation accuracy for small water bodies. To verify the effectiveness of our method, we chose the Yellow River Basin in China as the research area and used Sentinel-2 and Sentinel-1 satellite images as well as manually labelling samples to construct a dataset. On this dataset, our method achieves a 93.7% F1 score, which is a significant improvement compared with other methods. Finally, we use the proposed method to map the seasonal and permanent water bodies in the Yellow River Basin in 2021 and compare it with existing water bodies. The results show that our method has certain advantages in mapping large-scale water bodies, which not only ensures the overall integrity but also retains local details.
Spatial Transformation—The Importance of a Bottom-Up Approach in Creating Authentic Public Spaces
Authors: El Moussaoui Mustapha
Published: December 2023

This study explores the integration of phenomenology in urban placemaking, focusing on the Ghobeiry neighborhood in Beirut. By examining the transformation of a public garden through a phenomenological lens, this research highlights the impact of a bottom-up approach in urban design. The methodology combines a literature review with empirical data gathered from interviews and observations within the community. The findings indicate that the initial top-down development of the public garden failed to resonate with residents, leading to its neglect. However, a shift towards community engagement, initiated by a local social activist, encouraged a sense of ownership and transformed the space into a vibrant, meaningful area. This study contributes to urban planning literature by demonstrating the practical application of phenomenological principles, emphasizing the importance of community involvement in creating authentic urban spaces. It underscores the need for inclusive, participatory approaches in urban development, offering insights into the transformative potential of engaging local narratives and experiences.
Numerical Investigation of the Impact of Tall Buildings on Pollutant Dispersion during Stable Stratification
Authors: Li Yunpeng , Li Ruojie , Guo Dongpeng , Wang Dezhong , Pan Yanhui , Zhang Junfang , Yao Rentai
Published: December 2023

The present study employs the k-epsilon turbulence model to investigate the influence of stable stratification with different Richardson numbers (Rib) on flow patterns and pollutant dispersion near tall buildings. The results show that thermal stratification significantly affects the flow pattern around buildings. As Rib increases, the leeward stagnation point gradually shifts upward toward the top of the building, while the recirculation region on the top of the building moves downward, and the length of the recirculation region on the windward side initially increases and then decreases. The vortex position gradually moves above the building. The region with high TKE/uH2 is primarily concentrated on the top of the building and within the downwind recirculation area. As Rib increases, the TKE/uH2 decreases in the top and wake regions of the building. With increasing Rib, the ground-level pollutant concentration first increases and then decreases, the height of the downwind plume gradually reduces, while the maximum concentration in the plume rises.
Hindcast Insights from Storm Surge Forecasting of Super Typhoon Saola (2309) in Hong Kong with the Sea, Lake and Overland Surges from Hurricanes Model
Authors: Lau Dick-Shum , Chan Wai-Soen , Wong Yat-Chun , Lam Ching-Chi , Chan Pak-Wai
Published: December 2023

Super Typhoon Saola (2309) skirted past south-southeast of Hong Kong within 40 km on the night of 1 September 2023, posing a significant storm surge threat to Hong Kong. Given the close proximity of Saola with a peak intensity of about 210 km/h within 300 km of Hong Kong, a close call of the “super typhoon direct-hit” scenario, this case provides valuable insights from a hindcast review of storm surge forecasts and warning operation using the Sea, Lake and Overland Surges from Hurricanes (SLOSH) model, which is the operational storm surge model adopted by the Hong Kong Observatory (HKO). The performance of the HKO’s PRobabilistic Inundation Map Evaluation System (PRIMES) using both statistical and model ensemble approaches was also reviewed in this paper. Saola was a challenging case for operational forecasting of a compact TC structure with changes in storm size and intensity when it came close to Hong Kong. With major observations of storm structure using weather radar and dense automatic weather station, tide gauge and water level gauge networks, the high sensitivity of storm surge forecasts to the storm size parameter and the distance of closest approach was clearly revealed in the case of Saola. Even with a circularly symmetric TC parametric model like SLOSH, the hindcast review results illustrated that the model outputs were reasonably accurate during the closest approach of Saola given an accurate storm size and distance of closest approach were input, and using a highly computationally efficient storm surge model made it possible for the nowcasting of storm surges to handle compact and intense TC direct-hit cases in operational TC forecasting. Taking a nowcasting approach not only helps provide more reliable storm tide forecasts, but also facilitates the formulation of a better warning strategy when making final-call decisions in emergency response actions, based on the more frequent real-time analysis of TC position, intensity and storm size and the more accurate prediction of these parameters. A nowcasting workflow for storm surge operation was proposed in this paper.
Characterizing and Removing Ultra-Violet Contamination in Ion Observations on Board Tianwen-1
Authors: Wang Limin , Li Lei , Li Wenya , Kong Linggao , Tang Binbin , Ma Jijie , Zhang Yiteng , Xie Lianghai , Zhang Aibing , Qiao Fuhao , Wurz Peter , Galli André
Published: December 2023

The Mars Ion and Neutral Particle Analyzer (MINPA) onboard Tianwen-1 aims to study the interaction between Mars and the solar wind via in situ ion measurement and energetic neutral atom imaging. Despite the efforts for Ultra-Violet suppression in MINPA design, 0.48% of ion observations from November 2021 to July 2022 were identified as UV-contaminated. The UV emissions primarily penetrate into the instrument through the ENA entrance. Statistically, the distribution of the UV contamination in phase space typically spans 3 to 4 azimuth sectors. The contamination is uniformly distributed across the polar dimension while, in the energy and mass dimensions, it is proportional to the time-of-flight duration. Comparisons between the in-flight performance and ground calibration suggest that azimuthal broadening and intensity variations of the contamination may result from differing responses across the azimuthal sectors. Based on the characteristics of the UV impact on MINPA ion observations, a removal algorithm is proposed to reduce contamination while preserving valid signals, which improves the data quality effectively and benefits the interpretation of MINPA’s ion measurements in the Martian space environment. The cause, effect, and distribution of the UV contamination obtained by this study may serve as a reference for other space ion observations.
Non-Line-of-Sight Atmospheric Optical Communication in the Visible Wavelength Range between UAV and the Ground Surface
Authors: Tarasenkov Mikhail V. , Poznakharev Egor S. , Fedosov Andrey V.
Published: December 2023

An opto-electronic system for non-line-of-sight (NLOS) communication using scattered laser radiation for unmanned aerial vehicle (UAV)–ground and ground–UAV schemes at a wavelength of λ = 450 nm and a ground–UAV scheme at a wavelength of λ = 510 nm are described. The symbol error rate (SER) and its standard deviation were analyzed for different schemes of the communication channel. The transceiver system included a laser source with a power supply, a modulator, a lens refractor, a bandpass filter, a photomultiplier tube (PMT), a demodulator, and a receiving computer. The experimental data obtained at nighttime showed that the NLOS atmospheric optical communication at a wavelength of λ = 450 nm was feasible for the UAV–ground scheme at a baseline distance of up to 150 m for a UAV with a transmitter at a height of 10 m and at a baseline distance of up to 125 m for a UAV at a height of 20 m. For the ground–UAV scheme, stable communication was observed at baseline distances of up to 50 m for a UAV with a receiver at a height up to 30 m. The NLOS atmospheric optical communication at a wavelength of 510 nm was obtained for the ground–UAV scheme at baseline distances of up to 100 m for a UAV with a receiver at a height up to 45 m, as well as at baseline distances of up to 385 m for UAV flying at a height up to 20 m.
Paw-Type Characterization of Hourglass-Free Hamilton-Connected Graphs
Authors: Wang Panpan , Xiong Liming
Published: December 2023

This paper introduces the forbidden subgraph conditions for Hamilton-connected graphs. If the degree sequence of the graph is (4,2,2,2,2) and it is connected, then it is called hourglassΓ0. For integers i≥1, the graph Zi is paw, which is obtained by attaching one of the vertices of the triangle to one of the end vertices of a path with a number of edges i. We show that every graph G is Hamilton-connected if G is a Γ0-free, K1,3-free, Z14-free, and a 3-connected graph. Moreover, we give an example to show the sharpness of a paw-type forbidden subgraph in a 3-connected, Hamilton-connected graph. Our focus on the Hamilton-connected problem can be applied to data center networks (DCNs). In the future, we will remove the forbidden subgraph families from our conclusions when building the network to obtain the optimal communication cost. Our result extends the result of Ryjáček and Vrána (Discrete Mathematics 344: 112350, 2021).
Incorporating Socio-Economic Factors in Maximizing Two-Dimensional Demand Coverage and Minimizing Distance to Uncovered Demand: A Dual-Objective MCLP Approach for Fire Station Location Selection
Authors: Untadi Albertus , Li Lily D. , Li Michael , Dodd Roland
Published: December 2023

In this study, we employ a dual-objective optimization model, utilizing the Implicit Modified Coverage Location Problem (MCLP-Implicit) approach, to determine an appropriate fire station allocation. Our objectives encompass maximizing coverage in areas with a heightened projected demand, based on socioeconomic predictors of building fires, and concurrently minimizing the distance of uncovered demand zones to the closest fire station. The challenges of this model reflect the criticality of strategic placement, aiming for not just swift response times but also the complete coverage of a region with priority given to subregions with a pronounced potential for incidents. The applicability of the proposed approach is demonstrated through a case study in south-east Queensland. Our findings indicate that there is a tangible justification for adopting this model. The broader coverage and wider spread of locations it produces can greatly aid in the dynamic deployment of personnel during surges caused by seasonal fluctuations or unforeseen calamities. By weaving in socioeconomic aspects into demand predictions, our model has also maintained appropriate coverage to socioeconomically disadvantaged communities in the region. Nevertheless, it is paramount to underline the recommendation that further research should account for road connectivity—a pivotal factor when pinpointing these locations in the real world. This research paves the way for enriched insights in long-term urban planning and fire response protocol crafting, especially in regions mirroring similar socioeconomic profiles or risks of natural disasters, not to mention its commercial implications to the relevant industry servicing the fire and rescue authorities.
Epicardial Pulsed Field Ablation of Ganglionated Plexi: Computational and Pre-Clinical Evaluation of a Bipolar Sub-Xiphoid Catheter for the Treatment of Atrial Fibrillation
Authors: O’Brien Barry , Reilly John , Coffey Ken , González-Suárez Ana , Buchta Piotr , Buszman Piotr P. , Lukasik Karolina , Tri Jason , van Zyl Martin , Asirvatham Samuel
Published: December 2023

Epicardial pulsed field ablation (PFA) of ganglionated plexi (GPs) is being explored as a potential treatment for atrial fibrillation. Initial work using open-chest access with a monopolar ablation device has been completed. This study describes the early development work for a device that can be used with subxiphoid access and deliver bipolar ablation pulses. Electric field computational models have been used for the initial guidance on pulse parameters. An in vivo assessment of these ablation parameters has been performed in an open-chest canine study, while subxiphoid access and navigation of the device has been demonstrated in a porcine model. Results from this acute study have demonstrated the promising potential of this approach.
Blue and Red LED Lights Differently Affect Growth Responses and Biochemical Parameters in Lentil (Lens culinaris)
Authors: Bottiglione Benedetta , Villani Alessandra , Mastropasqua Linda , De Leonardis Silvana , Paciolla Costantino
Published: December 2023

Light-emitting diodes are an attractive tool for improving the yield and quality of plant products. This study investigated the effect of different light intensity and spectral composition on the growth, bioactive compound content, and antioxidant metabolism of lentil (Lens culinaris Medik.) seedlings after 3 and 5 days of LED treatment. Two monochromatic light quality × three light intensity treatments were tested: red light (RL) and blue light (BL) at photosynthetic photon flux density (PPFD) of 100, 300, and 500 μmol m−2 s−1. Both light quality and intensity did not affect germination. At both harvest times, the length of seedling growth under BL appeared to decrease, while RL stimulated the growth with an average increase of 26.7% and 62% compared to BL and seedlings grown in the darkness (D). A significant blue light effect was detected on ascorbate reduced form, with an average increase of 35% and 50% compared to RL-grown plantlets in the two days of harvesting, respectively. The content of chlorophyll and carotenoids largely varied according to the wavelength and intensity applied and the age of the seedlings. Lipid peroxidation increased with increasing light intensity in both treatments, and a strong H2O2 formation occurred in BL. These results suggest that red light can promote the elongation of lentil seedlings, while blue light enhances the bioactive compounds and the antioxidant responses.
Controlling Hair Loss by Regulating Apoptosis in Hair Follicles: A Comprehensive Overview
Authors: Wang Wuji , Wang Honglan , Long Yunluan , Li Zheng , Li Jingjie
Published: December 2023

Apoptosis is a physiological process that occurs in all cell types of the human body, and it profoundly changes the fate of hair by affecting hair follicle cells. This review outlines the cellular changes, intrinsic biochemical characteristics, and mechanisms underlying apoptosis and summarizes the hair follicle life cycle, including development, cycle stages, and corresponding cellular changes. Finally, the relationship between apoptosis and the hair cycle is discussed and the significance of apoptosis in hair loss conditions and drug treatments is highlighted. Apoptosis induces cellular changes and exhibits distinctive properties through intricate signaling pathways. Hair follicles undergo cyclic periods of growth, regression, and dormancy. Apoptosis is closely correlated with the regression phase by triggering hair follicle cell death and shedding. Regulation of apoptosis in hair follicles plays an essential role in hair loss due to maladies and drug treatments. Mitigating apoptosis can enhance hair growth and minimize hair loss. A comprehensive understanding of the correlation between apoptosis and the hair cycle can facilitate the development of novel treatments to prevent hair loss and stimulate hair regeneration.
Evidence of Guanidines Potential against Leishmania (Viannia) braziliensis: Exploring In Vitro Effectiveness, Toxicities and of Innate Immunity Response Effects
Authors: dos Anjos Luana Ribeiro , de Souza Vanessa Maria Rodrigues , Machado Yasmim Alves Aires , Partite Vitor Moreira , Aufy Mohammed , Dias Lopes Geovane , Studenik Christian , Alves Carlos Roberto , Lubec Gert , Gonzalez Eduardo Rene Perez , Rodrigues Klinger Antonio da Franca
Published: December 2023

Leishmaniasis is a complex group of infectious and parasitic diseases that afflict many thousands of individuals across five continents. Leishmaniasis treatment remains a challenge because it relies on drugsknown for their high toxicity and limited efficacy, making itimperative to identify new molecules that offer greater effectiveness and safety. This study sought to explore the impact of seven synthetic guanidine derivatives (LQOF-G1, LQOF-G2, LQOF-G6, LQOF-G7, LQOF-G32, LQOF-G35 and LQOF-G36) onthe parasite Leishmania (Viannia) braziliensis and in vitro macrophage infection by this parasite, as well as cytotoxic approaches in vitro models of mammalian host cells and tissues. The synthesized compounds showed purity ≥ 99.65% and effectively inhibited parasite growth. LQOF-G1 proved the most potent, yielding the best half-maximal inhibitory concentration (IC50) values against promastigotes (4.62 μmol/L), axenic amastigotes (4.27 μmol/L), and intracellular amastigotes (3.65 μmol/L). Notably, the antileishmanial activity of LQOF-G1, LQOF-G2, and LQOF-G6 was related to immunomodulatory effects, evidenced by alterations in TNF-α, IL-12, IL-10, nitric oxide (NO), and reactive oxygen species (ROS) levels in the supernatant of culture macrophages infected with L. (V.) braziliensis and coincubated with these compounds. LQOF-G2 and LQOF-G36 compounds exhibited vasodilator and spasmolytic effects at higher concentrations (≥100 μmol/L). Generally, LQOF-G1, LQOF-G2, and LQOF-G32 compounds were found to be nontoxic to assessed organs and cells. No toxic effects were observed in human cell lines, such as HEK-293, CaCo-2 and A549, at concentrations ≥ 500 μmol/L. Collectively, data have shown unequivocal evidence of the effectiveness of these compounds against L. (V.) braziliensis parasite, one of the causative agents of Tegumentary Leishmaniasis and Mucocutaneous Leishmaniasis in America.
High Phosphate-Induced JAK-STAT Signalling Sustains Vascular Smooth Muscle Cell Inflammation and Limits Calcification
Authors: Macrì Federica , Vigorito Ilaria , Castiglione Stefania , Faggiano Stefano , Casaburo Manuel , Fanotti Nadia , Piacentini Luca , Vigetti Davide , Vinci Maria Cristina , Raucci Angela
Published: December 2023

Vascular calcification (VC) is an age-related complication characterised by calcium-phosphate deposition in the arterial wall driven by the osteogenic transformation of vascular smooth muscle cells (VSMCs). The JAK-STAT pathway is an emerging target in inflammation. Considering the relationship between VC and inflammation, we investigated the role of JAK-STAT signalling during VSMC calcification. Human aortic smooth muscle cells (HASMCs) were cultured in high-inorganic phosphate (Pi) medium for up to 7 days; calcium deposition was determined via Alizarin staining and colorimetric assay. Inflammatory factor secretion was evaluated via ELISA and JAK-STAT members’ activation using Western blot or immunohistochemistry on HASMCs or calcified aortas of Vitamin D-treated C57BL6/J mice, respectively. The JAK-STAT pathway was blocked by JAK Inhibitor I and Von Kossa staining was used for calcium deposits in murine aortic rings. During Pi-induced calcification, HASMCs released IL-6, IL-8, and MCP-1 and activated JAK1-JAK3 proteins and STAT1. Phospho-STAT1 was detected in murine calcified aortas. Blocking of the JAK-STAT cascade reduced HASMC proliferation and pro-inflammatory factor expression and release while increasing calcium deposition and osteogenic transcription factor RUNX2 expression. Consistently, JAK-STAT pathway inhibition exacerbates mouse aortic ring calcification ex vivo. Intriguingly, our results suggest an alternative link between VSMC inflammation and VC.
Chronic Thromboembolic Pulmonary Hypertension: A Review of the Multifaceted Pathobiology
Authors: Ghani Hakim , Pepke-Zaba Joanna
Published: December 2023

Chronic thromboembolic pulmonary disease results from the incomplete resolution of thrombi, leading to fibrotic obstructions. These vascular obstructions and additional microvasculopathy may lead to chronic thromboembolic pulmonary hypertension (CTEPH) with increased pulmonary arterial pressure and pulmonary vascular resistance, which, if left untreated, can lead to right heart failure and death. The pathobiology of CTEPH has been challenging to unravel due to its rarity, possible interference of results with anticoagulation, difficulty in selecting the most relevant study time point in relation to presentation with acute pulmonary embolism (PE), and lack of animal models. In this article, we review the most relevant multifaceted cross-talking pathogenic mechanisms and advances in understanding the pathobiology in CTEPH, as well as its challenges and future direction. There appears to be a genetic background affecting the relevant pathological pathways. This includes genetic associations with dysfibrinogenemia resulting in fibrinolysis resistance, defective angiogenesis affecting thrombus resolution, and inflammatory mediators driving chronic inflammation in CTEPH. However, these are not necessarily specific to CTEPH and some of the pathways are also described in acute PE or deep vein thrombosis. In addition, there is a complex interplay between angiogenic and inflammatory mediators driving thrombus non-resolution, endothelial dysfunction, and vascular remodeling. Furthermore, there are data to suggest that infection, the microbiome, circulating microparticles, and the plasma metabolome are contributing to the pathobiology of CTEPH.
How to Generate Self-Efficacy despite Pain: The Role of Catastrophizing and Avoidance in Women with Fibromyalgia
Authors: Catalá Patricia , Gutiérrez Lorena , Écija Carmen , Peñacoba Cecilia
Published: December 2023

Background and Objective: Fibromyalgia-related pain is influenced by numerous factors, including severity, as well as cognitive profiles based on pain catastrophizing or activity patterns. In this context, self-efficacy is identified as a potential predictor for explaining certain health outcomes. This study aimed to contribute to exploring the role of pain avoidance (as activity pattern) between pain severity and self-efficacy along pain catastrophizing. Methods: Through a cross-sectional study, a total of 264 women with fibromyalgia completed self-report measures of pain severity, pain avoidance, pain catastrophizing, and self-efficacy. The severity of the symptoms, the time elapsed since diagnosis, and the time elapsed since the onsets of symptoms were included as covariates to control. Regression-based moderated-mediation analysis was used to test the conditional effect of pain severity on self-efficacy via pain avoidance at varying levels of pain catastrophizing. Results: Pain avoidance mediated the effect of pain severity on self-efficacy. The indirect effects showed a moderated effect when patients scored high on the pain catastrophizing scale. The model evaluated, where catastrophic pain moderates the indirect effect of pain intensity on self-efficacy through pain avoidance, explained 49% of the variance. Conclusions: Catastrophic beliefs associated with pain as being uncontrollable increase the relationship between pain severity and pain avoidance. In turn, pain avoidance is associated with a low perception of capacity.
Increased Levels of VCAM-1 in Patients with High Cardiovascular Risk and Obstructive Sleep Apnea Syndrome
Authors: Chetan Ioana-Maria , Vesa Ștefan Cristian , Domokos Gergely Bianca , Beyer Ruxandra Stefana , Tomoaia Raluca , Cabau Georgiana , Vulturar Damiana Maria , Pop Dana , Todea Doina
Published: December 2023

(1) Background: Although obstructive sleep apnea (OSA) is associated with increased cardiovascular morbidity, the link between OSA and cardiovascular disease (CVD) is not completely elucidated. Thus, we aim to assess cardiovascular risk (CVR) using SCORE 2 and SCORE 2 for older persons (SCORE 2OP), and to evaluate the association between the endothelial biomarkers VCAM-1, ICAM-1, epicardial fat, and sleep study parameters in order to improve current clinical practices and better understand the short-and long-term CVRs in OSA patients. (2) Methods: 80 OSA patients and 37 healthy volunteers were enrolled in the study. SCORE2 and SCORE 2 OP regional risk charts (validated algorithms to predict the 10-year risk of first-onset CVD) were used for the analysis of CVR. Two-dimensional echocardiography was performed on all patients and epicardial fat thickness was measured. VCAM-1 and ICAM-1 serum levels were assessed in all patients. (3) Results: OSA patients were classified as being at high CVR, regardless of the type of score achieved. Increased EFT was observed in the OSA group. VCAM-1 was associated with a high CVR in OSA patients, but no significant correlation was observed between adhesion molecules and epicardial fat thickness. (4) Conclusions: OSA patients have a high CVR according to the SCORE 2 and SCORE 2OP risk scores. VCAM-1 may be associated with a high CVR in OSA patients. Extending conventional risk stratification scores by adding other potential biomarkers improves the risk stratification and guide treatment eligibility for CVD prevention in the OSA population.
The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening
Authors: Shkrigunov Timur , Zgoda Victor , Klimenko Peter , Kozlova Anna , Klimenko Maria , Lisitsa Andrey , Kurtser Mark , Petushkova Natalia
Published: December 2023

Problems with the male reproductive system are of both medical and social significance. As a rule, spermatozoa and seminal plasma proteomes are investigated separately to assess sperm quality. The current study aimed to compare ejaculate proteomes with spermatozoa and seminal plasma protein profiles regarding the identification of proteins related to fertility scores. A total of 1779, 715, and 2163 proteins were identified in the ejaculate, seminal plasma, and spermatozoa, respectively. Among these datasets, 472 proteins were shared. GO enrichment analysis of the common proteins enabled us to distinguish biological processes such as single fertilization (GO:0007338), spermatid development (GO:0007286), and cell motility (GO:0048870). Among the abundant terms for GO cellular components, zona pellucida receptor complex, sperm fibrous sheath, and outer dense fiber were revealed. Overall, we identified 139 testis-specific proteins. For these proteins, PPI networks that are common in ejaculate, spermatozoa, and seminal plasma were related to the following GO biological processes: cilium movement (GO:0003341), microtubule-based movement (GO:0007018), and sperm motility (GO:0097722). For ejaculate and spermatozoa, they shared 15 common testis-specific proteins with spermatogenesis (GO:0007283) and male gamete generation (GO:0048232). Therefore, we speculated that ejaculate-based proteomics could yield new insights into the peculiar reproductive physiology and spermatozoa function of men and potentially serve as an explanation for male infertility screening.
Scaffold Hopping and Structural Modification of NSC 663284: Discovery of Potent (Non)Halogenated Aminobenzoquinones
Authors: Bayrak Nilüfer , Sever Belgin , Ciftci Halilibrahim , Otsuka Masami , Fujita Mikako , TuYuN Amaç Fatih
Published: December 2023

The development of new anticancer drugs is still ongoing as a solution to the unsatisfactory results obtained by chemotherapy patients. Our previous studies on natural product-based anticancer agents led us to synthesize a new series of Plastoquinone (PQ) analogs and study their anticancer effects. Four members of PQ analogs (PQ1–4) were designed based on the scaffold hopping strategy; the design was later completed with structural modification. The obtained PQ analogs were synthesized and biologically evaluated against different cancer genotypes according to NCI-60 screening in vitro. According to the NCI results, bromo and iodo-substituted PQ analogs (PQ2 and PQ3) showed remarkable anticancer activities with a wide-spectrum profile. Among the two selected analogs (PQ2 and PQ3), PQ2 showed promising anticancer activity, in particular against leukemia cell lines, at both single- and five-dose NCI screenings. This compound was also detected by MTT assay to reveal significant selectivity between Jurkat cells and PBMC (healthy) compared to imatinib. Further in silico studies indicated that PQ2 was able to occupy the ATP-binding cleft of Abl TK, one of the main targets of leukemia, through key interactions similar to dasatinib and imatinib. PQ2 is also bound to the minor groove of the double helix of DNA. Based on computational pharmacokinetic studies, PQ2 possessed a remarkable drug-like profile, making it a potential anti-leukemia drug candidate for future studies.
Auditory Cortex Asymmetry Associations with Individual Differences in Language and Cognition
Authors: Eckert Mark A. , Vaden Kenneth I. , Paracchini Silvia
Published: December 2023

A longstanding cerebral lateralization hypothesis predicts that disrupted development of typical leftward structural asymmetry of auditory cortex explains why children have problems learning to read. Small sample sizes and small effects, potential sex-specific effects, and associations that are limited to specific dimensions of language are thought to have contributed inconsistent results. The large ABCD study dataset (baseline visit: N = 11,859) was used to test the hypothesis of significant associations between surface area asymmetry of auditory cortex and receptive vocabulary performance across boys and girls, as well as an oral word reading effect that was specific to boys. The results provide modest support (Cohen’s d effect sizes ≤ 0.10) for the cerebral lateralization hypothesis.
The Light Cupula Phenomenon: A Scoping Review
Authors: Lee Dong-Han , Kim Tae Hee , Jang Minho , Kim Chang-Hee
Published: December 2023

Direction-changing positional nystagmus (DCPN), which refers to the change in the direction of nystagmus with different head positions, is a well-known characteristic of horizontal semicircular canal BPPV. The supine head roll test is commonly used to diagnose horizontal canal BPPV. However, persistent geotropic DCPN observed during this test cannot be explained by the conventional explanations of canalolithiasis or cupulolithiasis. To account for this unique nystagmus, the concept of a “light cupula” has been recently introduced. In this review, we provide an overview of the historical background, clinical features and diagnostic methods, proposed mechanisms, and treatment strategies associated with the light cupula phenomenon based on the available literature to date.
Efficacy of Using Intermittent Theta Burst Stimulation to Treat Negative Symptoms in Patients with Schizophrenia—A Systematic Review and Meta-Analysis
Authors: Tan Xiaowei , Goh Shih Ee , Lee Jonathan Jie , Vanniasingham Sean David , Brunelin Jérôme , Lee Jimmy , Tor Phern Chern
Published: December 2023

Negative symptoms in schizophrenia impose a significant burden with limited effective pharmacological treatment options. Recent trials have shown preliminary evidence for the efficacy of using intermittent theta burst stimulation (iTBS) in treating negative symptoms in schizophrenia. We aim to systematically review the current evidence of iTBS in the treatment of the negative symptoms of schizophrenia as an augmentation therapy. The study protocol was developed and registered on Prospero (registration ID: 323381). MEDLINE, EMBASE, Web of Science (Scopus), PsycINFO and Wan Fang databases were searched for sham-controlled, randomized trials of iTBS among patients with schizophrenia. The mean difference in major outcome assessments for negative symptoms was calculated. The quality of evidence was assessed using the Cochrane Risk of Bias Tool (version 1) and the GRADE system. Moreover, 12 studies including a total of 637 participants were included. Compared to sham treatment, the pooled analysis was in favor of iTBS treatment for negative symptoms (mean weight effect size: 0.59, p = 0.03) but not for positive symptoms (mean weight effect size: 0.01, p = 0.91) and depressive symptoms (mean weight effect size: 0.35, p = 0.16). A significant treatment effect was also observed on the iTBS target site left dorsal prefrontal cortex (mean weight effect size: 0.86, p = 0.007) and for stimulation with 80% motor threshold (mean weight effect size: 0.86, p = 0.02). Thus, our synthesized data support iTBS as a potential treatment for negative symptoms among patients with schizophrenia. However, the long-term efficacy and safety issues of iTBS in a larger population have yet to be examined.
Neuroinflammation and Neurodegenerative Diseases: How Much Do We Still Not Know?
Authors: Balistreri Carmela Rita , Monastero Roberto
Published: December 2023

The term “neuroinflammation” defines the typical inflammatory response of the brain closely related to the onset of many neurodegenerative diseases (NDs). Neuroinflammation is well known, but its mechanisms and pathways are not entirely comprehended. Some progresses have been achieved through many efforts and research. Consequently, new cellular and molecular mechanisms, diverse and conventional, are emerging. In listing some of those that will be the subject of our description and discussion, essential are the important roles of peripheral and infiltrated monocytes and clonotypic cells, alterations in the gut–brain axis, dysregulation of the apelinergic system, alterations in the endothelial glycocalyx of the endothelial component of neuronal vascular units, variations in expression of some genes and levels of the encoding molecules by the action of microRNAs (miRNAs), or other epigenetic factors and distinctive transcriptional factors, as well as the role of autophagy, ferroptosis, sex differences, and modifications in the circadian cycle. Such mechanisms can add significantly to understanding the complex etiological puzzle of neuroinflammation and ND. In addition, they could represent biomarkers and targets of ND, which is increasing in the elderly.
A Special Issue on the Roles of Dopamine in Neural Circuits, Genetics, and Behavior
Authors: Boschen Suelen L. , D’Aquila Paolo S. , Lester Deranda B.
Published: December 2023

Over the past 80 years, research on dopamine has undergone significant evolution, reshaping our understanding of its roles in the brain and the body [...]
Clinical, Sociodemographic, and Psychological Factors Associated with Transition Readiness in Patients with Epilepsy
Authors: Vacca Mariacarolina , Fernandes Mariana , Veronese Lorenzo , Ballesio Andrea , Cerminara Caterina , Galasso Cinzia , Mazzone Luigi , Lombardo Caterina , Mercuri Nicola Biagio , Liguori Claudio
Published: December 2023

Background: The transition to adult care for patients with epilepsy is a complicated clinical issue associated with adverse outcomes, including non-adherence to treatment, dropout of medical care, and worse prognosis. Moreover, youngsters with epilepsy are notably prone to emotional, psychological, and social difficulties during the transition to adulthood. Transition needs depend on the type of epilepsy and the epileptic syndrome, as well as on the presence of co-morbidities. Having a structured transition program in place is essential to reduce poor health consequences. A key strategy to optimize outcomes involves the use of transition readiness and associated factors assessment to implement the recognition of vulnerability and protective aspects, knowledge, and skills of these patients and their parents. Therefore, this study aims to provide a comprehensive framework of clinical and psychosocial aspects associated with the transition from pediatric to adult medical care of patients with epilepsy. Methods: Measures examining different aspects of transition readiness and associated clinical, socio-demographic, psychological, and emotional factors were administered to 13 patients with epilepsy (Mage = 22.92, SD = 6.56) with (n = 6) or without (n = 7) rare diseases, and a respective parent (Mage = 56.63, SD = 7.36). Results: patients showed fewer problems in tracking health issues, appointment keeping, and pharmacological adherence as well as low mood symptoms and moderate resiliency. Moreover, they referred to a low quality of sleep. Notably, parents of patients with rare diseases reported a lower quality of sleep as compared to the other group of parents. Conclusions: Increasing awareness around transition readiness is essential to promote self-management skills of patients with epilepsy and their parents. Anticipating the period of transition could be beneficial, especially to prevent problematic sleep patterns and promote independence in health care management. Parents of patients with epilepsy and rare diseases should be monitored for their mental status which can affect patients’ well-being.
New Insight into the Possible Roles of L-Carnitine in a Rat Model of Multiple Sclerosis
Authors: Safwat Sally M. , Aboonq Moutasem Salih , El Tohamy Mahmoud , Mojaddidi Moaz , Al-Qahtani Saeed Awad M. , Zakari Madaniah Omar , ElGendy Ahmed A. , Hussein Abdelaziz M.
Published: December 2023

Objective: We investigated the effect of L-carnitine (LC) on cuprizone (Cup) demyelinating rat model and its possible underlying mechanisms. Methods: Thirty male Sprague–Dawley (SD) rats were randomly allocated to three groups: the normal control group; the Cup group, in which Cup was administrated at a dose of 450 mg/kg per day orally via gastric gavage for 5 weeks; and the Cup + LC group, which received the same dose of Cup as the Cup group, except that the rats were treated additionally with LC 100 mg/kg/day orally for 5 weeks. The nerve conduction (NCV) in isolated sciatic nerves was measured; then, the sciatic nerves were isolated for H&E staining and electron microscope examination. The expression of myelin basic protein (MBP), IL-1β, p53, iNOS, and NF-KB by immunohistochemistry was detected in the isolated nerves. A PCR assay was also performed to detect the expression of antioxidant genes Nrf2 and HO-1. In addition, the level of IL-17 was measured by ELISA. Results: There was a significant reduction in NCV in the Cup group compared to normal rats (p < 0.001), which was significantly improved in the LC group (p < 0.001). EM and histopathological examination revealed significant demyelination and deterioration of the sciatic nerve fibers, with significant improvement in the LC group. The level of IL-17 as well as the expression of IL-1β, p53, iNOS, and NF-KB were significantly increased, with significant reduction expression of MBP in the sciatic nerves (p < 0.01), and LC treatment significantly improved the studied parameters (p < 0.01). Conclusion: The current study demonstrates a neuroprotective effect of LC in a Cup-induced demyelinating rat model. This effect might be due to its anti-inflammatory and antioxidant actions.
Early Risk Factors for Self-Injurious Thoughts and Behaviours: A UK Population-Based Study of 219,581 People
Authors: Porras-Segovia Alejandro , Pascual-Sanchez Ana , Greenfield Geva , Creese Hanna-Marie , Saxena Sonia , Hargreaves Dougal , Nicholls Dasha
Published: December 2023

Mental disorders are a major problem among young people. To identify early risk factors of self-injurious thoughts and behaviours (SITB) among young adults with mental health problems, this case–control study drew data from the Clinical Practice Research Datalink (CPRD), a primary care database covering 8% of the UK population. We explored the role of early factors (presenting at 8–14 years old) for suicidal ideation, suicide attempts, and non-suicidal self-injury (NSSI) in young adulthood (age 18–25 years) by performing logistic regressions. Our sample consisted of 219,581 participants, of which 6.51% had at least one SITB in young adulthood. Early risk factors for SITB included early NSSI, suicidal ideation, sexual abuse, behavioural problems, and mood and psychotic symptoms. Frequency of GP visits had a protective effect. Lack of access to mortality data, ethnicity, and socioeconomic status was a limitation of the current study. In conclusion, early symptoms in late childhood/early adolescence can be the start of long-standing problems going into adult life. The training of primary care providers in suicide risk assessment and proper co-ordination with child and adolescent mental health services are crucial for suicide prevention.
Study on Controlled Low-Strength Materials Using Ultra-Rapid-Hardening Cement and Stone Sludge for Backfill and Subbase Application in Road Excavation and Restoration Work
Authors: Lee Jongwon , Baek Cheolmin
Published: December 2023

A significant amount of stone sludge is generated as a by-product during the production of crushed stone aggregate, and most of it is disposed of in landfill as waste. In order to recycle this stone sludge, this study evaluated a controlled low-strength material (CLSM) using ultra-rapid-hardening cement and stone sludge for application as backfill and subbase material for road excavation and restoration work. In addition, considering the limited construction time of excavation and restoration work in urban areas, backfill and subbase materials must simultaneously satisfy conditions of fluidity, workability, quick curing time, and certain levels of strength. Therefore, in this study, CLSM was manufactured according to various mixing ratios and flow, slump, and compressive strength tests with age were evaluated. Additionally, the change trend in the microstructure of the CLSM with age was analyzed. Through indoor experiments, the optimal mixing ratios for backfill and subbase CLSM were determined, and field applicability and performance of field samples were evaluated through small-scale field construction. It was concluded that CLSM, which contains a large amount of stone sludge, can be sufficiently applied as a backfill and subbase material for excavation and restoration work if appropriate admixtures are adjusted according to the weather conditions at sites.