bluuebunny/crossref_metadata_2025 huggingface dataset:
Stability of Imbalanced Triangles in Gene Regulatory Networks of Cancerous and Normal Cells
Authors: Rizi Abbas Karimi , Zamani Mina , Shirazi Amirhossein , Jafari G. Reza , Kertész János
Published: January 2021

Genes communicate with each other through different regulatory effects, which lead to the emergence of complex network structures in cells, and such structures are expected to be different for normal and cancerous cells. To study these differences, we have investigated the Gene Regulatory Network (GRN) of cells as inferred from RNA-sequencing data. The GRN is a signed weighted network corresponding to the inductive or inhibitory interactions. Here we focus on a particular of motifs in the GRN, the triangles, which are imbalanced if the number of negative interactions is odd. By studying the stability of imbalanced triangles in the GRN, we show that the network of cancerous cells has fewer imbalanced triangles compared to normal cells. Moreover, in the normal cells, imbalanced triangles are isolated from the main part of the network, while such motifs are part of the network's giant component in cancerous cells. Our result demonstrates that due to genes' collective behavior the structure of the complex networks is different in cancerous cells from those in normal ones.
Effect of Running Exercise on Oxidative Stress Biomarkers: A Systematic Review
Authors: Thirupathi Anand , Pinho Ricardo A. , Ugbolue Ukadike C. , He Yuhuan , Meng Yao , Gu Yaodong
Published: January 2021

Background: Exercise induced health benefits are limited by the overaccumulation of reactive oxygen species (ROS). ROS and further oxidative stress could potentially induce muscle damage which could result in poor exercise performance. However, predicting ROS induced oxidative stress in response to endurance training has several limitations in terms of selecting biomarkers that are used to measure oxidative stress.

Objective: The purpose of this study was to systematically investigate the suitable biomarkers that predict oxidative stress status among runners.

Methods: According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement, a search for relevant articles was carried out on PubMed/Medline, ISI Web of Science, and Google Scholar using related search terms such as oxidative damage, ROS, exercise, physical training, running, marathon, and ultramarathon.

Results: Outcomes included (1) running programs like a half-marathon, ultramarathon, and iron-man race, (2) measuring biochemical assessment of oxidative damage markers such as malondialdehyde (MDA), protein carbonyl (PC), total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS), 8-Oxo-2'-deoxyguanosine (8-OH-dG), 4-hydroxynonenal (HNE), and F1-isoprostones, and enzymatic and non-enzymatic antioxidants level.

Conclusions: This study concluded that a running exercise does not elicit a response to specific biomarkers of oxidative stress, instead, oxidative damage markers of lipids, proteins, and various enzymatic and non-enzymatic antioxidants are expressed according to the training status of the individual.
Pacing in World-Class Age Group Swimmers in 200 and 400 m Individual Medley
Authors: Moser Cathia , Sousa Caio Victor , Olher Rafael Reis , Hill Lee , Nikolaidis Pantelis Theodoros , Rosemann Thomas , Knechtle Beat
Published: January 2021

The present research investigated pacing for world-class age group swimmers competing in individual medley in 200 m and 400 m. Data on 3,242 unique finishers (1,475 women and 1,767 men) competing in four Master World Championships [XV FINA WMC held in Montreal (Canada) in 2014, the XVI FINA WMC held in Kazan (RUS) in 2015, the FINA WMC held in Budapest (HUN) in 2017, and the XVIII FINA WMC held in Gwangju (KOR] in 2019) were analyzed. Men were faster than women among all age groups in both 200 and 400 m. Additionally, differences were found between almost all adjacent age groups, with the exception (p > 0.05) of age groups 25–29 to 30–34, 35–39 to 40–44 years in 200 m races and 25–29 to 30–34, 30–34 to 35–39, 35–39 to 40–44, and 45–49 to 50–54 years in 400 m races. Men showed a higher pacing variation in 200 m among all male age groups and all female age groups up to 69 years. Pace-variation pairwise comparisons between men and women showed no consistencies throughout age groups, with the exception of a higher variation in men in age groups ≥55-year-old. Men were faster for all splits and strokes in both 200 and 400 m, and significant changes were identified for each split and stroke for both men and women in both 200 and 400 m. Front crawl (freestyle, 4th split) was the fastest butterfly (1st split), backstroke (2nd split), and breaststroke (3rd split). In summary, men were faster than women for all age groups in both 200 and 400 m. Men showed a higher pacing variation in 200 m in all age groups, where women had a higher variation in age groups up to 69 years. The fastest stroke for the final spurt was front crawl, followed by butterfly, backstroke, and breaststroke. Based on these findings, coaches should advise their master athletes to focus on the final spurt in both 200 and 400 m individual medley for a fast final race time.
Metabolomics Evaluation of Patients With Stage 5 Chronic Kidney Disease Before Dialysis, Maintenance Hemodialysis, and Peritoneal Dialysis
Authors: Zhu Sang , Zhang Feng , Shen Ai-Wen , Sun Bo , Xia Tian-Yi , Chen Wan-Sheng , Tao Xia , Yu Sheng-Qiang
Published: January 2021

Objective

Current treatment options for patients with stage 5 chronic kidney disease before dialysis (predialysis CKD-5) are determined by individual circumstances, economic factors, and the doctor’s advice. This study aimed to explore the plasma metabolic traits of patients with predialysis CKD-5 compared with maintenance hemodialysis (HD) and peritoneal dialysis (PD) patients, to learn more about the impact of the dialysis process on the blood environment.

Methods

Our study enrolled 31 predialysis CKD-5 patients, 31 HD patients, and 30 PD patients. Metabolite profiling was performed using a targeted metabolomics platform by applying an ultra-high-performance liquid chromatography-tandem mass spectrometry method, and the subsequent comparisons among all three groups were made to explore metabolic alterations.

Results

Cysteine metabolism was significantly altered between predialysis CKD-5 patients and both groups of dialysis patients. A disturbance in purine metabolism was the most extensively changed pathway identified between the HD and PD groups. A total of 20 discriminating metabolites with large fluctuations in plasma concentrations were screened from the group comparisons, including 2-keto-D-gluconic acid, kynurenic acid, s-adenosylhomocysteine, L-glutamine, adenosine, and nicotinamide.

Conclusion

Our study provided a comprehensive metabolomics evaluation among predialysis CKD-5, HD, and PD patients, which described the disturbance of metabolic pathways, discriminating metabolites and their possible biological significances. The identification of specific metabolites related to dialysis therapy might provide insights for the management of advanced CKD stages and inform shared decision-making.
Planthopper-Secreted Salivary Disulfide Isomerase Activates Immune Responses in Plants
Authors: Fu Jianmei , Shi Yu , Wang Lu , Zhang Hao , Li Jing , Fang Jichao , Ji Rui
Published: January 2021

The small brown planthopper (Laodelphax striatellus; SBPH) is a piercing-sucking insect that secretes salivary proteins into its plant host during feeding. However, the mechanisms by which these salivary proteins regulate plant defense responses remain poorly understood. Here, we identified the disulfide isomerase (LsPDI1) in the SBPH salivary proteome. LsPDI1 was highly expressed in the SBPH salivary glands and secreted into rice plants during feeding. Transient in planta LsPDI1 expression in the absence of signal peptide induced reactive oxygen species (ROS) burst, cell death, callose deposition, and jasmonic acid (JA) signaling pathway. Deletion mutant analysis revealed that either the a-b-b’ or the b-b’-a’ domains in LsPDI1 are required to induce cell death in plants. LsPDI1 and its orthologs were highly conserved among various planthopper species and strongly induced ROS burst and cell death in plants. Transient in Nicotiana benthamiana LsPDI1 expression impaired the performance of Spodoptera frugiperda and Myzus persicae on host plants. Hence, LsPDI1 is an important salivary elicitor that enhances plant resistance to insects by inducing the calcium, ROS, and JA signaling pathways. The findings of this study provide novel insights into the molecular mechanisms underlying plant-insect interactions.
Repeat Traffic Offenders Improve Their Performance in Risky Driving Situations and Have Fewer Accidents Following a Mindfulness-Based Intervention
Authors: Baltruschat Sabina , Mas-Cuesta Laura , Cándido Antonio , Maldonado Antonio , Verdejo-Lucas Carmen , Catena-Verdejo Elvira , Catena Andrés
Published: January 2021

Risky decision-making is highly influenced by emotions and can lead to fatal consequences. Attempts to reduce risk-taking include the use of mindfulness-based interventions (MBI), which have shown promising results for both emotion regulation (ER) and risk-taking. However, it is still unclear whether improved emotion regulation is the mechanism responsible for reduced risk-taking. In the present study, we explore the effect of a 5-week MBI on risky driving in a group of repeat traffic offenders by comparing them with non-repeat offenders and repeat offenders without training. We evaluated the driving behavior of the participants through a driving simulation, and self-reported emotion regulation, both before and after the intervention. At baseline, poor emotion regulation was related to a more unstable driving behavior, and speeding. The group that received mindfulness training showed improved performance during risky driving situations and had fewer accidents, although their overall driving behavior remained largely unchanged. The observed trend toward improved emotion regulation was not significant. We discuss whether other effects of MBI – such as self-regulation of attention – could underlie the observed reduction in risky driving in the initial stages. Nonetheless, our findings still confirm the close relationship between emotion regulation skills and risky driving.
Study Protocol for the Evaluation of Individual Psychological Interventions for Family Caregivers of Advanced Cancer Patients
Authors: Yang Min , Sun Rui , Wang Yanfeng , Xu Haiyan , Zou Baohua , Yang Yanmin , Cong Minghua , Zheng Yadi , Yu Lei , Ma Fei , Qiu Tinglin , Li Jiang
Published: January 2021

Background: Both anxiety and depression in family caregivers (FCs) of advanced cancer patients are common, and they have a negative influence on both the FCs and the patients. Some studies suggested that a variety of interventions could alleviate the psychological symptoms of FCs. However, there is no consensus on much more effective methods for intervention, and relatively high-quality research is blank in psychological problems of these population in China. The validity of mindfulness-based stress reduction (MBSR) and psychological consultation guided by the needs assessment tool (NST) in the psychological status of caregivers will be compared in this study to select a more suitable intervention for the FCs of advanced cancer patients in China.

Methods and Analysis: A randomized N-of-1 trial would be conducted at the Cancer Hospital, Chinese Academy of Medical Sciences. Fifty eligible FCs of advanced cancer patients will be recruited, and all will receive three cycles of psychological intervention treatment, with each cycle including both of MBSR and psychological consultation guided by the NST. MBSR and psychological consultation guided by the NST will be compared with each other in each cycle, and the intervention sequence will be based on the random number table generated after the informed consent has been completed. Each treatment period is 2 weeks, and the interval between different treatment cycles or treatment periods is 1 week. The self-reported scales are measured at the beginning and end of each treatment period, including the Self-Rating Anxiety Scale (SAS), the Self-Rating Depression Scale (SDS), Distress Thermometer (DT), Zarit Burden Interview (ZBI), Chinese version of the Medical Outcomes Study 12-item Short Form (C-SF-12), and Family Carer Satisfaction with Palliative Care scale (FAMCARE-2).

Dissemination: The protocol of the study was approved by the Institutional Review Board of the Ethical Committee of the Cancer Hospital, Chinese Academic of Medical Science. The results will be published in a peer-reviewed medical journal. The study is registered at Chinese Clinical Trials Registry with the trial registration number chiCTR2000033707. This study employs an innovative methodological approach on the effectiveness of MBSR and psychological consultation guided by the NST for psychological status of FCs of advanced cancer patients. The findings of the study will be helpful to provide high-quality evidence-based medical data for psychological intervention of FCs of advanced cancer patients, and guide clinicians on best quality treatment recommendations.
Indian Public Health Students' Perspectives on Global Health Education
Authors: Sawleshwarkar Shailendra , Zodpey Sanjay P. , Negin Joel
Published: January 2021

Global health discipline is of increasing interest for educators and students in public health across the world. Public health education is recently gaining momentum in India, but global health is still at an embryonic stage. Value of students as stakeholders in curriculum development is increasingly recognized but literature about perspectives of public health students regarding global health education is limited. This study aimed to explore Indian public health students' perspectives about global health education and to provide platform for the development of global health education framework for future public health professionals. This study involved a series of focus groups with students and sought to understand perceptions about global health and global health education framework. We recruited public health students at three institutes across India for focus group discussions. Focus groups questions covered current understanding of global health, opinions regarding global health education for public health curriculum and the relevance of global health competency domains for future employment. Recordings were transcribed verbatim and the transcripts were read along with field notes and then analyzed thematically. A total of 36 students participated in four focus groups. There was a general recognition that global health is transnational and that a global outlook is now essential. But there were concerns regarding local and global priorities in public health. Global health was regarded as being wider than public health by some, but others viewed public health being the umbrella term with global health as a specialization. Global health competencies were viewed as a “step up” from the public health competencies but core public health competencies were considered essential. International experiences and use of technology were key themes for delivery of global health education. Employability and career progression for global health graduates were of concern for many participants. This study provides insight into the student perspectives regarding global health education for public health programs in India. Clear direction in terms of curriculum and its utility for career growth and employability as a global health professional needs to be established for global health education in India and other similar settings.
The Crossroads of Periodontitis and Oral Squamous Cell Carcinoma: Immune Implications and Tumor Promoting Capacities
Authors: Elebyary Omnia , Barbour Abdelahhad , Fine Noah , Tenenbaum Howard C. , Glogauer Michael
Published: January 2021

Periodontitis (PD) is increasingly considered to interact with and promote a number of inflammatory diseases, including cancer. In the case of oral squamous cell carcinoma (OSCC) the local inflammatory response associated with PD is capable of triggering altered cellular events that can promote cancer cell invasion and proliferation of existing primary oral carcinomas as well as supporting the seeding of metastatic tumor cells into the gingival tissue giving rise to secondary tumors. Both the immune and stromal components of the periodontium exhibit phenotypic alterations and functional differences during PD that result in a microenvironment that favors cancer progression. The inflammatory milieu in PD is ideal for cancer cell seeding, migration, proliferation and immune escape. Understanding the interactions governing this attenuated anti-tumor immune response is vital to unveil unexplored preventive or therapeutic possibilities. Here we review the many commonalities between the oral-inflammatory microenvironment in PD and oral-inflammatory responses that are associated with OSCC progression, and how these conditions can act to promote and sustain the hallmarks of cancer.
Drinking Carling Out of Stella Glasses: People and Place in the Missing Middle
Authors: McEwan Kathryn
Published: January 2021

As trends of social and economic change allow precarity to inch into the lives of those who may have been more accustomed to security (Standing,2011,2014), this paper addresses the response of some young people who are caught “betwixt and between” in potentially liminal states (Turner,1967). Those whose families have undertaken intra- or intergenerational social mobility and who have made a home in a place, Ingleby Barwick in Teesside, that seems to be of them and for them—an in-between place that is seen as “not quite” middle or working class. This paper draws data from a research project that adopted a qualitative phenomenological approach to uncover the meaning of experiences for participants. Methods included focus groups and semi-structured interviews through which 70 local people contributed their thoughts, hopes, concerns, and stories about their lives now and what they aspire to for the future. Places, such as the large private housing estate in the Northeast of England on which this research was carried out, make up significant sections of the UK population, yet tend to be understudied populations, often missed by a sociological gaze attracted to extremes. It was anticipated that in Ingleby Barwick, where social mobility allows access to this relatively exclusive estate, notions of individualism and deservingness that underlie meritocratic ideology (Mendick et al.,2015; Littler,2018) would be significant, a supposition borne out in the findings. “Making it” to Ingleby was, and continues to be, indicative to many of meritocratic success, making it “a moral place for moral people” (McEwan,2019). Consequently, the threat then posed by economic precarity, of restricting access to the transitions and lifestyles that create the “distinction” (Bourdieu,1984) required to denote fit to this place, is noted to be very real in a place ironically marked by many outside it as fundamentally unreal.
Pastoralism at Scale on the Kazakh Rangelands: From Clans to Workers to Ranchers
Authors: Kerven Carol , Robinson Sarah , Behnke Roy
Published: January 2021

Eurasia contains the world's largest contiguous rangelands, grazed for millennia by mobile pastoralists' livestock. This paper reviews evidence from one Eurasian country, Kazakhstan, on how nomadic pastoralism developed from some 5,000 years ago to the present. We consider a timespan covering pre-industrial, socialist and capitalist periods, during which pastoral social formations were organized in terms of kinship, collective state farms, and private farms and ranches. The aim is to understand how events over the last 100 years have led to the sequential dissolution and re-formation of the social units necessary to manage livestock across a wide expanse of spatially heterogenous and seasonally variable rangeland ecosystems. It is argued that the social scale of extensive livestock management must be tailored to the geographical scale of biotic and abiotic conditions. The paper starts by pointing out the long duration of mobile pastoralism in the Kazakh rangelands and provides an overview of how events from the late 17th C onwards unraveled the relationships between Kazakh nomads' socio-economic units of livestock management and the rangeland environment. At present, mobile animal husbandry is not feasible for the majority of Kazakh livestock owners, who operate solely within small family units without state support. These reformulated post-Soviet livestock grazing patterns are still undergoing rapid change, influencing the composition of rangeland vegetation, wildlife biodiversity, and rates of carbon sequestration. By concentrating capital and landed resources, a minority of large-scale pastoralists have been able to re-extensify by combining mobility with selective intensification, including an increased reliance on cultivated feed. Current state and international efforts are leaving out the majority of small-scale livestock owners and their livestock who are unable to either intensify or extensify at sufficient scale, increasing environmental damage, and social inequality.
Greenhouse Gas Emissions Trends and Mitigation Measures in Australian Agriculture Sector—A Review
Authors: Panchasara Heena , Samrat Nahidul , Islam Nahina
Published: January 2021

Agriculture is an important source of greenhouse gas emissions. It is one of the economic sectors that impacts both directly and indirectly towards climate change which contributes to greenhouse gas emissions. There has been a continuous trend of agricultural greenhouse gas emissions reduction technologies, but any step taken in this direction must not negatively affect farm productivity and economics. For the agriculture sector to achieve reduced GHG emission, climate-smart activities and improved food security will be needed for this sector to become a climate-smart landscape. Climate-smart technologies are effective at targeting inputs to the fields, helping to lower greenhouse gas emissions. This article explores the key sources of carbon emissions within the agriculture sector and reviews efficient ways to GHG emission via Smart Farming technology. Based on the public archive GHG datasets, we have found that livestock farming is the largest GHG emission sector among other agricultural sectors and responsible for 70% of the total emission. Besides, we also show that Queensland is the largest agricultural GHG contributor compared to other states and territories. The article also captures any possible sources within smart farming that may contribute to carbon emissions and suggest ways to reduce GHG emissions. Besides, an Australian-based best management practice approach is discussed to review the emissions reduction strategy based on climate-specific technology to help the farmers and other stakeholders take environmentally-friendly agricultural decisions.
Antioxidant and Anti-Inflammatory Activity of Citrus Flavanones Mix and Its Stability after In Vitro Simulated Digestion
Authors: Denaro Marcella , Smeriglio Antonella , Trombetta Domenico
Published: January 2021

Recently, several studies have highlighted the role of Citrus flavanones in counteracting oxidative stress and inflammatory response in bowel diseases. The aim of study was to identify the most promising Citrus flavanones by a preliminary antioxidant and anti-inflammatory screening by in vitro cell-free assays, and then to mix the most powerful ones in equimolar ratio in order to investigate a potential synergistic activity. The obtained flavanones mix (FM) was then subjected to in vitro simulated digestion to evaluate the availability of the parent compounds at the intestinal level. Finally, the anti-inflammatory activity was investigated on a Caco-2 cell-based model stimulated with interleukin (IL)-1β. FM showed stronger antioxidant and anti-inflammatory activity with respect to the single flavanones, demonstrating the occurrence of synergistic activity. The LC-DAD-ESI-MS/MS analysis of gastric and duodenal digested FM (DFM) showed that all compounds remained unchanged at the end of digestion. As proof, a superimposable behavior was observed between FM and DFM in the anti-inflammatory assay carried out on Caco-2 cells. Indeed, it was observed that both FM and DFM decreased the IL-6, IL-8, and nitric oxide (NO) release similarly to the reference anti-inflammatory drug dexamethasone.
Dietary Polyphenols in Metabolic and Neurodegenerative Diseases: Molecular Targets in Autophagy and Biological Effects
Authors: García-Aguilar Ana , Palomino Olga , Benito Manuel , Guillén Carlos
Published: January 2021

Polyphenols represent a group of secondary metabolites of plants which have been analyzed as potent regulators of multiple biological processes, including cell proliferation, apoptosis, and autophagy, among others. These natural compounds exhibit beneficial effects and protection against inflammation, oxidative stress, and related injuries including metabolic diseases, such as cardiovascular damage, obesity and diabetes, and neurodegeneration. This review aims to summarize the mechanisms of action of polyphenols in relation to the activation of autophagy, stimulation of mitochondrial function and antioxidant defenses, attenuation of oxidative stress, and reduction in cell apoptosis, which may be responsible of the health promoting properties of these compounds.
Functionalization of Betulinic Acid with Polyphenolic Fragments for the Development of New Amphiphilic Antioxidants
Authors: Sousa Joana L. C. , Gonçalves Cristiana , Ferreira Ricardo M. , Cardoso Susana M. , Freire Carmen S. R. , Silvestre Armando J. D. , Silva Artur M. S.
Published: January 2021

The present work aimed at the valorization of biomass derived compounds by their transformation into new added-value compounds with enhanced antioxidant properties. In this context, betulinic acid (BA) was decorated with polyphenolic fragments, and polyhydroxylated (E)-2-benzylidene-19,28-epoxyoleanane-3,28-diones 4a–d were obtained. For that, the synthetic strategy relied on base-promoted aldol condensation reactions of methyl betulonate, which was previously prepared from natural BA, with appropriate benzaldehydes, followed by cleavage of the methyl protecting groups with BBr3. It is noteworthy that the HBr release during the work-up of the cleavage reactions led to the rearrangement of the lupane-type skeleton of the expected betulonic acid derivatives into oleanane-type compounds 4a–d. The synthesized compounds 4a–d were designed to have specific substitution patterns at C-2 of the triterpene scaffold, allowing the establishment of a structure-activity relationship. The radical scavenging ability of 4a–d was evaluated using the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS•+) scavenging assays. In particular, derivative 4c, bearing a catechol unit, revealed to be the most efficient scavenger against both free radicals DPPH• and ABTS•+. Subsequently, we designed two analogues of the hit derivative 4c in order to achieve more potent antioxidant agents: (i) the first analogue carries an additional unsaturation in its lateral chain at C-2 (analogue 5) and (ii) in the second analogue, E-ring was kept in its open form (analogue 6). It was observed that the presence of an extended π-conjugated system at C-2 contributed to an increased scavenging effect, since analogue 5 was more active than 6, α-tocopherol, and 4c in the ABTS•+ assay.
Unique Cellular and Biochemical Features of Human Mitochondrial Peroxiredoxin 3 Establish the Molecular Basis for Its Specific Reaction with Thiostrepton
Authors: Nelson Kimberly J. , Messier Terri , Milczarek Stephanie , Saaman Alexis , Beuschel Stacie , Gandhi Uma , Heintz Nicholas , Smalley Terrence L. , Lowther W. Todd , Cunniff Brian
Published: January 2021

A central hallmark of tumorigenesis is metabolic alterations that increase mitochondrial reactive oxygen species (mROS). In response, cancer cells upregulate their antioxidant capacity and redox-responsive signaling pathways. A promising chemotherapeutic approach is to increase ROS to levels incompatible with tumor cell survival. Mitochondrial peroxiredoxin 3 (PRX3) plays a significant role in detoxifying hydrogen peroxide (H2O2). PRX3 is a molecular target of thiostrepton (TS), a natural product and FDA-approved antibiotic. TS inactivates PRX3 by covalently adducting its two catalytic cysteine residues and crosslinking the homodimer. Using cellular models of malignant mesothelioma, we show here that PRX3 expression and mROS levels in cells correlate with sensitivity to TS and that TS reacts selectively with PRX3 relative to other PRX isoforms. Using recombinant PRXs 1–5, we demonstrate that TS preferentially reacts with a reduced thiolate in the PRX3 dimer at mitochondrial pH. We also show that partially oxidized PRX3 fully dissociates to dimers, while partially oxidized PRX1 and PRX2 remain largely decameric. The ability of TS to react with engineered dimers of PRX1 and PRX2 at mitochondrial pH, but inefficiently with wild-type decameric protein at cytoplasmic pH, supports a novel mechanism of action and explains the specificity of TS for PRX3. Thus, the unique structure and propensity of PRX3 to form dimers contribute to its increased sensitivity to TS-mediated inactivation, making PRX3 a promising target for prooxidant cancer therapy.
A Comparative Investigation on Phenolic Composition, Characterization and Antioxidant Potentials of Five Different Australian Grown Pear Varieties
Authors: Wang Zening , Barrow Colin J. , Dunshea Frank R. , Suleria Hafiz A. R.
Published: January 2021

Pear (Pyrus communis L.) is widely spread throughout the temperate regions of the world, such as China, America and Australia. This fruit is popular among consumers due to its excellent taste and perceived health benefits. Various bioactive compounds, which contribute to these health benefits, have been detected in the pear fruits, including a range of phenolic compounds. Five Australian grown pear varieties, which include Packham’s Triumph, Josephine de Malines, Beurre Bosc, Winter Nelis and Rico were selected for this study to examine the phenolic compounds in pears. Beurre Bosc exhibited the highest total polyphenol content (TPC) (3.14 ± 0.02 mg GAE/g), total tannin content (TTC) (1.43 ± 0.04 mg CE/g) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) (5.72 ± 0.11 mg AAE/g), while the Josephine de Malines variety was high in total flavonoid content (TFC) (1.53 ± 0.09 mg QE/g), ferric reducing antioxidant power (FRAP) (4.37 ± 0.04 mg AAE/g), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (4.44 ± 0.01 mg AAE/g) and total antioxidant capacity (TAC) (5.29 ± 0.09 mg AAE/g). The liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS) data indicate that a total of 73 phenolic compounds were detected in Beurre Bosc (37 compounds), Josephine de Malines (34), Rico (22), Packham’s Triumph (15) and Winter Nelis (9), respectively. From HPLC-PDA quantification, the Beurre Bosc pear variety showed significantly higher in phenolic acids (chlorogenic acid; 17.58 ± 0.88 mg/g) and while flavonoids were significantly higher in Josephine de Malines (catechin; 17.45 ± 1.39 mg/g), as compared to other pear varieties. The analyses suggest that the Australian grown pears might contain an ideal source of phenolic compounds which benefit human health. The information provided by the present work can serve as practical supporting data for the use of pears in the nutraceutical, pharmaceutical and food industries.
Analysis of Circuit Simulation Considering Total Ionizing Dose Effects on FinFET and Nanowire FET
Authors: Won Hyeonjae , Kang Myounggon
Published: January 2021

In this study, we analyzed the total ionizing dose (TID) effect characteristics of p-type FinFET and Nanowire FET (NW-FET) according to the structural aspect through comparison of the two devices. Similar to n-type devices, p-type NW-FETs are less affected than FinFETs by the TID effect. For the inverter TID circuit simulation, both n- and p-types of FinFET and NW-FET were analyzed regarding the TID effect. The inverter operation considering the TID effect was verified using the Berkeley short-channel insulated-gate FET model (BSIM) common multi-gate (CMG) parameters. In addition, an inverter circuit composed of the NW-FET exhibited a smaller change by the TID than that of an inverter circuit composed of the FinFET. Therefore, the gate controllability of the gate-all-around (GAA) device had an excellent tolerance to not only short-channel effects (SCE) but also TID effects.
Nonlinear Dynamic Analysis of Seismically Base-Isolated Structures by a Novel OpenSees Hysteretic Material Model
Authors: Vaiana Nicoló , Capuano Raffaele , Sessa Salvatore , Marmo Francesco , Rosati Luciano
Published: January 2021

The complex response characterizing elastomeric isolation bearings is reproduced by employing a novel uniaxial hysteretic model that has been recently formulated and successfully implemented in OpenSees. Such a novel OpenSees material model offers several advantages with respect to differential models typically available in commercial software products for structural analysis, such as 3D-BASIS and CSi programs. Firstly, it is based on a set of only five model parameters that have a clear mechanical significance; such a property not only allows one to drastically simplify the parameters identification process, but it also allows the model to be used in practice. In addition, the model does not require numerical methods for the evaluation of the restoring force since the latter is computed by solving an algebraic equation. To encourage researchers and designers to adopt the proposed model for research and practical purposes, we demonstrate its accuracy by performing some numerical tests in OpenSees. In particular, we first employ the recently implemented model to compute the nonlinear dynamic response of a seismically base-isolated structure with elastomeric bearings and, subsequently, we compare the results with those obtained by modeling the seismic isolators with the OpenSees BoucWen uniaxial material model, that is one of the most popular and accurate hysteretic models currently available in OpenSees.
Improvement of Bacterial Vaginosis by Oral Lactobacillus Supplement: A Randomized, Double-Blinded Trial
Authors: Lin Ta-Chin , Hsu I-Ling , Tsai Wan-Hua , Chu Yi-Chih , Kuan Lung-Ching , Huang Min-Syuan , Yeh Wen-Ling , Chen Ya-Hui , Hsu Shan-Ju , Chang Wen-Wei
Published: January 2021

Bacterial vaginosis (BV) is the most common vaginal infection globally, with a high recurrent rate after antibiotic treatment. Probiotics consumption is known to improve BV with different efficacy among species or strains. After in vitro selection of Lactobacillus strains with growth inhibition and preventing adhesion to HeLa cervical epithelial cells, a randomized and double-blinded trial of two Lactobacillus formula, namely, VGA-1 and VGA-2, in BV patients with Nugent scores of 4–10 was conducted. Among 37 subjects who completed the trial, we observed significantly decreased Nugent scores in both VGA-1 (n = 18) and VGA-2 (n = 19) consumption groups. VGA-1 consumption significantly improved vaginal discharge odor/color and itching at both 2-week and 4-week-consumption, but those only observed after a 4-week-consumption in the VGA-2 group. We also observed a tendency to reduce recurrent rates among enrolled participants after VGA-1 or VGA-2 consumption. The improvement effect of VGA-1/VGA-2 was associated with the significant reduction of interleukin-6 expression after 4-week-consumption and the restoration of normal vaginal microflora by quantitative polymerase chain reaction analysis. In conclusion, VGA-1 or VGA-2 displayed beneficial effects in BV patients, but the VGA-1 formula showed a better efficacy, potentially used for BV intervention.
A Smart and Sustainable Future for Viticulture Is Rooted in Soil: How to Face Cu Toxicity
Authors: Cesco Stefano , Pii Youry , Borruso Luigimaria , Orzes Guido , Lugli Paolo , Mazzetto Fabrizio , Genova Giulio , Signorini Marco , Brunetto Gustavo , Terzano Roberto , Vigani Gianpiero , Mimmo Tanja
Published: January 2021

In recent decades, agriculture has faced the fundamental challenge of needing to increase food production and quality in order to meet the requirements of a growing global population. Similarly, viticulture has also been undergoing change. Several countries are reducing their vineyard areas, and several others are increasing them. In addition, viticulture is moving towards higher altitudes and latitudes due to climate change. Furthermore, global warming is also exacerbating the incidence of fungal diseases in vineyards, forcing farmers to apply agrochemicals to preserve production yields and quality. The repeated application of copper (Cu)-based fungicides in conventional and organic farming has caused a stepwise accumulation of Cu in vineyard soils, posing environmental and toxicological threats. High Cu concentrations in soils can have multiple impacts on agricultural systems. In fact, it can (i) alter the chemical-physical properties of soils, thus compromising their fertility; (ii) induce toxicity phenomena in plants, producing detrimental effects on growth and productivity; and (iii) affect the microbial biodiversity of soils, thereby influencing some microbial-driven soil processes. However, several indirect (e.g., management of rhizosphere processes through intercropping and/or fertilization strategies) and direct (e.g., exploitation of vine resistant genotypes) strategies have been proposed to restrain Cu accumulation in soils. Furthermore, the application of precision and smart viticulture paradigms and their related technologies could allow a timely, localized and balanced distribution of agrochemicals to achieve the required goals. The present review highlights the necessity of applying multidisciplinary approaches to meet the requisites of sustainability demanded of modern viticulture.
The Effectiveness of Ensemble-Neural Network Techniques to Predict Peak Uplift Resistance of Buried Pipes in Reinforced Sand
Authors: Zeng Jie , Asteris Panagiotis G. , Mamou Anna P. , Mohammed Ahmed Salih , Golias Emmanuil A. , Armaghani Danial Jahed , Faizi Koohyar , Hasanipanah Mahdi
Published: January 2021

Buried pipes are extensively used for oil transportation from offshore platforms. Under unfavorable loading combinations, the pipe’s uplift resistance may be exceeded, which may result in excessive deformations and significant disruptions. This paper presents findings from a series of small-scale tests performed on pipes buried in geogrid-reinforced sands, with the measured peak uplift resistance being used to calibrate advanced numerical models employing neural networks. Multilayer perceptron (MLP) and Radial Basis Function (RBF) primary structure types have been used to train two neural network models, which were then further developed using bagging and boosting ensemble techniques. Correlation coefficients in excess of 0.954 between the measured and predicted peak uplift resistance have been achieved. The results show that the design of pipelines can be significantly improved using the proposed novel, reliable and robust soft computing models.
Low-Cost Calibration of Matching Error between Lidar and Motor for a Rotating 2D Lidar
Authors: Yuan Chang , Bi Shusheng , Cheng Jun , Yang Dongsheng , Wang Wei
Published: January 2021

For a rotating 2D lidar, the inaccurate matching between the 2D lidar and the motor is an important error resource of the 3D point cloud, where the error is shown both in shape and attitude. Existing methods need to measure the angle position of the motor shaft in real time to synchronize the 2D lidar data and the motor shaft angle. However, the sensor used for measurement is usually expensive, which can increase the cost. Therefore, we propose a low-cost method to calibrate the matching error between the 2D lidar and the motor, without using an angular sensor. First, the sequence between the motor and the 2D lidar is optimized to eliminate the shape error of the 3D point cloud. Next, we eliminate the attitude error with uncertainty of the 3D point cloud by installing a triangular plate on the prototype. Finally, the Levenberg–Marquardt method is used to calibrate the installation error of the triangular plate. Experiments verified that the accuracy of our method can meet the requirements of the 3D mapping of indoor autonomous mobile robots. While we use a 2D lidar Hokuyo UST-10LX with an accuracy of ±40 mm in our prototype, we can limit the mapping error within ±50 mm when the distance is no more than 2.2996 m for a 1 s scan (mode 1), and we can limit the mapping error within ±50 mm at the measuring range 10 m for a 16 s scan (mode 7). Our method can reduce the cost while the accuracy is ensured, which can make a rotating 2D lidar cheaper.
Understanding the Changed Mechanisms of Laser Beam Fusion Cutting by Applying Beam Oscillation, Based on Thermographic Analysis
Authors: Pinder Thomas , Goppold Cindy
Published: January 2021

The latest research on applying beam oscillation in laser beam fusion cutting revealed significant process improvements regarding speed and quality. The reason for this increasing process efficiency remains unexplained; however, theoretical investigations suggest the change in energy deposition (respectively heat conduction) as the cause. The present paper aims to analyze the energy deposition by a novel temperature measurement method. For this purpose, a conventional laser beam cutting setup was equipped with beam oscillation technology and a high-speed temperature measurement setup. Various characteristics of the temperature distribution in the process zone (spatial and temporal resolved temperature profiles, maximum and average values, as well as melt pool size) were evaluated for different conditions of beam oscillation (amplitude, frequency, cutting speed). Additionally, the geometrical properties of the process zone, defining the absorptivity have been measured. The comparison with static beam shaping reveals strong temperature volatility, which is induced by the way of energy deposition and an improved absorptivity over a substantial part of the cut front, with the overall result of enhanced heat conduction. For the first time, changed mechanisms applying beam oscillation instead of static beam shaping have been experimentally identified. Based on these measurements, a previously developed explanatory model was not only confirmed but also extended.
Development of a Biodegradable Microcarrier for the Cultivation of Human Adipose Stem Cells (hASCs) with a Defined Xeno- and Serum-Free Medium
Authors: Muoio Francesco , Panella Stefano , Lindner Matias , Jossen Valentin , Harder Yves , Moccetti Tiziano , Eibl Regine , Müller Michele , Tallone Tiziano
Published: January 2021

Stirred single-use bioreactors in combination with microcarriers (MCs) have established themselves as a technology that has the potential to meet the demands of current and future cell therapeutic markets. However, most of the published processes have been performed using fetal bovine serum (FBS) containing cell culture medium and non-biocompatible MCs. This approach has two significant drawbacks: firstly, the inevitable potential risks associated with the use of FBS for clinical applications; secondly, non-biocompatible MCs have to be removed from the cell suspension before implantation, requiring a step that causes loss of viable cells and adds further costs and complications. This study aimed to develop a new platform based on a chemically defined xeno- and serum-free cell culture medium and biodegradable MC that can support the growth of human adipose stem cells (hASCs) while still preserving their undifferentiated status. A specific combination of components and manufacturing parameters resulted in a MC prototype, called “BR44”, which delivered the desired functionality. MC BR44 allows the hASCs to stick to its surface and grow in a chemically defined xeno- and serum-free medium (UrSuppe). Although the cells’ expansion rate was not as high as with a commercial non-biodegradable standard MC, those cultured on BR44 maintained a better undifferentiated status in both static and dynamic conditions than those cultured on traditional 2D surfaces.
Correlation between Soil Bacterial Community Structure and Soil Properties in Cultivation Sites of 13-Year-Old Wild-Simulated Ginseng (Panax ginseng C.A. Meyer)
Authors: Kim Kiyoon , Kim Hyun Jun , Jeong Dae Hui , Huh Jeong Hoon , Jeon Kwon Seok , Um Yurry
Published: January 2021

Soil properties are one of the major factors determining the growth of vegetation. These properties drive the selection of the dominant bacterial community profiles, which eventually determines the soil quality and fertility. The abundance of preferential bacterial community assists in better productivity of a particular type of vegetation. The increasing focus on the health and well-being of the human population has resulted in a shift in paradigm to concentrate on the cultivation of medicinal plants such as Wild-simulated ginseng (WSG). These plant species take a long time for their growth and are generally cultivated in the mountainous forest trenches of Far East countries like South Korea. This study was conducted to decipher the bacterial community profiles and their correlation with soil chemical properties, which would give a broader idea about the optimum growing conditions of such an important medicinal plant. The important edaphic factor determined in this study was the soil pH, which was recorded to be acidic in all the studied cultivation sites. In agreement with the edaphic factor, the relative abundance of Acidobacteria was found to be highest as this phylum prefers to grow in acidic soils. Moreover, the total organic matter, total nitrogen and cation exchange capacity were found to be significantly correlated with the bacterial community. Hence, these results will help to identify the suitable cultivation sites for WSG and increase the productivity of these medicinal plants.
New Generalized Ekeland’s Variational Principle, Critical Point Theorems and Common Fuzzy Fixed Point Theorems Induced by Lin-Du’s Abstract Maximal Element Principle
Authors: Zhao Junjian , Du Wei-Shih
Published: January 2021

In this paper, by applying the abstract maximal element principle of Lin and Du, we present some new existence theorems related with critical point theorem, maximal element theorem, generalized Ekeland’s variational principle and common (fuzzy) fixed point theorem for essential distances.
RhoA- and Actin-Dependent Functions of Macrophages from the Rodent Cardiac Transplantation Model Perspective -Timing Is the Essence
Authors: Kloc Malgorzata , Uosef Ahmed , Villagran Martha , Zdanowski Robert , Kubiak Jacek Z. , Wosik Jarek , Ghobrial Rafik M.
Published: January 2021

The small GTPase RhoA, and its down-stream effector ROCK kinase, and the interacting Rac1 and mTORC2 pathways, are the principal regulators of the actin cytoskeleton and actin-related functions in all eukaryotic cells, including the immune cells. As such, they also regulate the phenotypes and functions of macrophages in the immune response and beyond. Here, we review the results of our and other’s studies on the role of the actin and RhoA pathway in shaping the macrophage functions in general and macrophage immune response during the development of chronic (long term) rejection of allografts in the rodent cardiac transplantation model. We focus on the importance of timing of the macrophage functions in chronic rejection and how the circadian rhythm may affect the anti-chronic rejection therapies.