bluuebunny/crossref_metadata_2025 huggingface dataset:
Early Predictive Value of NT-proBNP Combined With Echocardiography in Anthracyclines Induced Cardiotoxicity
Authors: Dong Yingjun , Wu Qiong , Hu Changqing
Published: July 2022

Objective

Determine the predictive value of N-terminal pro-B-type natriuretic peptide (NT-proBNP) combined with echocardiography in the diagnosis of anthracyclines-induced chronic cardiotoxicity.

Methods

A total of 80 female breast cancer patients from January 2019 to October 2021 were included in our hospital. Twenty-six patients with cardiotoxicity were divided into the cardiac impairment group, and the 54 patients without cardiotoxicity were classified into the normal control group. NT-proBNP levels and cardiac echocardiography were measured before the start of the chemotherapy cycle, in cycle 3 of the chemotherapy, and after the chemotherapy cycle in all patients.

Results

After three cycles of chemotherapy and chemotherapy, the levels of NT-proBNP in patients of the two groups were significantly higher than those before chemotherapy (P < 0.05). The levels of NT-proBNP in the cardiac injury group after three cycles of chemotherapy and chemotherapy were higher than those in the normal control group at the same time point (P < 0.05). The LVEF of patients in the cardiac impairment group after chemotherapy was lower than that before chemotherapy, and the LVEF after chemotherapy was lower than that in the normal control group (P < 0.05). NT-proBNP had a negative correlation with LVEF (r = −0.549, P < 0.001). The AUC of NT-proBNP in combination with LVEF for predicting cardiotoxicity in our patient was 0.898(95%CI:0.829–0.966).

Conclusion

NT-proBNP combined with echocardiography has clinical significance in the detection of anthracyclines-induced cardiotoxicity, and it can detect early myocardial injury induced by anthracyclines, with early prediction value. It is important to protect heart function and judge prognosis.
Same Dosages of rPRV/XJ5-gI−/gE−/TK− Prototype Vaccine or Bartha-K61 Vaccine Similarly Protects Growing Pigs Against Lethal Challenge of Emerging vPRV/XJ-5 Strain
Authors: Ren Qinghai , Li Lin , Pan Haochun , Wang Xiaobo , Gao Qingqing , Huan Changchao , Wang Jin , Zhang Wei , Jiang Luyao , Gao Song , Kai Yan , Chen Changhai
Published: July 2022

Variant pseudorabies viruses (vPRV) have constantly emerged in China since late 2011. In the present study, a 1 × 106.0 TCID50 per-animal dosage of a commercially available Bartha-K61 vaccine and an rPRV/XJ5-gI−/gE−/TK− prototype vaccine freshly extracted from the vPRV/XJ-5 at the same dose were administered to evaluate the immune effectiveness thereof on growing pigs to prevent lethal strikes caused by vPRV/XJ-5. The results suggest that the Bartha-K61 vaccine at a dose of 1 × 106.0 TCID50 per animal and the same dosage of the rPRV/XJ5-gI−/gE−/TK− prototype vaccine protected growing pigs against the lethal challenge of vPRV/XJ-5 strain with 100% survive rate. Furthermore, the outcome of the clinical score, virus shedding, weight gain, and viral loads in different pig tissues in these two groups demonstrates that either the Bartha-K61 vaccine or the rPRV/XJ5-gI−/gE−/TK− prototype vaccine at the same dose exhibited parallel efficacy in pigs against the lethal challenge with the XJ-5 strain of vPRV.
Understanding R Gene Evolution in Brassica
Authors: Zhang Fangning , Neik Ting Xiang , Wu Tingting , Edwards David , Batley Jacqueline
Published: July 2022

Brassica crop diseases caused by various pathogens, including viruses, bacteria, fungi and oomycetes, have devastating effects on the plants, leading to significant yield loss. This effect is worsened by the impact of climate change and the pressure to increase cultivation worldwide to feed the burgeoning population. As such, managing Brassica diseases has become a challenge demanding a rapid solution. In this review, we provide a detailed introduction of the plant immune system, discuss the evolutionary pattern of both dominant and recessive disease resistance (R) genes in Brassica and discuss the role of epigenetics in R gene evolution. Reviewing the current findings of how R genes evolve in Brassica spp. provides further insight for the development of creative ideas for crop improvement in relation to breeding sustainable, high quality, disease-resistant Brassica crops.
Mitigation of GHG Emissions from Soils Fertilized with Livestock Chain Residues
Authors: Lagomarsino Alessandra , Valagussa Massimo , Scotti Carla , Borrelli Lamberto , Becagli Claudia , Tosca Alberto
Published: July 2022

Using livestock residues as alternative fertilizers is a sustainable practice which recycles nutrients that would otherwise be lost. However, organic fertilizers may have a large impact on N2O emissions, offsetting the beneficial effects of C sequestration. After four years from biochar application, greenhouse gas fluxes were monitored for two years from a Maize field fertilized with digestate, slurry, or urea, with and without biochar. The objectives of the present study were to assess (i) the climate feedback of using residues from the livestock chain as alternative fertilizers and (ii) the contribution of biochar in mitigating GHGs emissions, while increasing the organic C in soil. Digestate was shown to have the highest impact on CO2 and N2O emissions from soil, with respect to mineral fertilization (+29 and +142%), more than slurry (+21 and −5%), whereas both residues positively affected CH4 uptake (+5 and +14%, respectively). The maximum N2O peaks occurred between 7–20 days after fertilization, accounting for 61% of total emissions, on average. Biochar was effective in reducing N2O emissions derived from mineral fertilization and digestate (−54% and −17%, respectively). An excess of labile organic matter and N induced the highest CO2 emissions and N2O peaks, independent of—or even triggered by—biochar. Mitigation of GHG emissions, from soils fertilized with livestock chain residue, can be obtained using biochar, but with limitations dependent on (i) the quantity of organic matter added, (ii) its quality, and (iii) the time from application: those aspects that deserve further investigations.
Reconstruction of Daily Courses of SO42−, NO3−, NH4+ Concentrations in Precipitation from Cumulative Samples
Authors: Hůnová Iva , Brabec Marek , Malý Marek , Škáchová Hana
Published: July 2022

It is important to study precipitation chemistry to comprehend both atmospheric and environmental processes. The aim of this study was the reconstruction of daily concentration patterns of major ions in precipitation from samples exposed for longer and differing time periods. We explored sulphates (SO42−), nitrates (NO3−) and ammonium (NH4+) ions measured in precipitation within a nation-wide atmospheric deposition monitoring network in the Czech Republic during 1980–2020. We visualised the long-term trends at selected individual years for four stations, Praha 4-Libuš (LIB), Svratouch (SVR), Rudolice v Horách (RUD) and Souš (SOU), differing in geographical location and reflecting different environments. We found anticipated time trends reflecting the emission patterns of the precursors, i.e., sharp decreases in SO42−, milder decreases in NO3− and steady states in NH4+ concentrations in precipitation. Statistically significant decreasing time trends in SO42− and NO3− concentrations in precipitation between 1990 and 2015 were revealed for the LIB and SVR sites. Spring maxima in April were found for all major ions at the LIB site and for NO3− for the SVR site, for both past and current samples, whereas no distinct seasonal behaviour was recorded for NH4+ at the RUD and SO42− at the SVR sites. By applying Bayesian modelling and the Integrated Nested Laplace Approximation approach, we were able to reconstruct the daily patterns of SO42−, NO3− and NH4+ concentrations in precipitation, which might be further utilised for a wide range of tasks, including comparison of magnitudes and shapes between stations, grouping the decomposed daily data into the ecologically motivated time periods, as well as for logical checks of sampling and measurement reliability.
Precision Medicine in Solid Tumors: How Far We Traveled So Far?
Authors: Dey Nandini , De Pradip
Published: July 2022

The future of disease management in solid tumors will rely heavily on how effectively we understand precision medicine and how successfully we can deliver personalized medicine [...]
Concurrent Androgen Deprivation Therapy for Prostate Cancer Improves Survival for Synchronous or Metachronous Non-Small Cell Lung Cancer: A SEER–Medicare Database Analysis
Authors: Nazha Bassel , Zhang Chao , Chen Zhengjia , Ragin Camille , Owonikoko Taofeek K.
Published: July 2022

Introduction: The crosstalk between receptor kinase signaling, such as EGFR and androgen receptor signaling, suggests a potential interaction between androgen deprivation therapy (ADT) and lung cancer outcome. Methods: We employed the SEER–Medicare data of lung cancer patients diagnosed between 1988 and 2005 to test for an association between ADT for prostate cancer and lung cancer outcome. We employed the Kaplan–Meier method and Cox proportional hazard with log-rank test model to assess any significant impact of ADT on survival. Results: We included data from 367,750 lung cancer patients; 17.4%, 2.9%, 33.6% and 46.1% with stages I, II, III and IV, respectively; 84.5% were >65 years; 57.2% males; 84.2% Caucasians and 9.3% Blacks. There were 11,061 patients (3%) with an initial prostate cancer diagnosis followed by lung cancer (P-L group); 3017 (0.8%) with an initial diagnosis of lung cancer and subsequent prostate cancer diagnosis (L-P group); the remainder had only lung cancer (L group). Stage I lung cancer was most common in the L-P group compared to the L and P-L groups—54% vs. 17.13% vs. 17.92%, p < 0.0001 for L-P, L and P-L, respectively. The median OS for lung cancer diagnosis was 93 months versus 10 and 9 months, respectively, for the L-P, L and P-L subgroups. ADT was associated with improved survival on multivariate analysis, especially in Caucasian patients (HR of death: 0.86; 95% CI: 0.76–0.97; p = 0.012). Conclusion: ADT was associated with improved outcome for NSCLC, in line with the hypothesis of a role for the androgen receptor in lung cancer. Our findings support a systematic evaluation of the potential benefit of ADT as a therapy for lung cancer.
Association of Energy Expenditure and Efficacy in Metastatic Renal Cell Carcinoma Patients Treated with Nivolumab
Authors: Noel Johanna , Jouinot Anne , Alexandre Jérôme , Ulmann Guillaume , Bretagne Marie , Castel-Ajgal Zahra , De Percin Sixtine , Vaquin-Villeminey Clémentine , Revel Marie-Pierre , Peyromaure Michael , Boudou-Rouquette Pascaline , Arrondeau Jennifer , Gataa Ithar , Durand Jean-Philippe , Goldwasser François , Huillard Olivier
Published: July 2022

Background: Nivolumab improved patients’ survival in metastatic renal cell carcinoma (mRCC). We aimed to evaluate resting energy expenditure (REE) (i.e., patients’ basal metabolism) to predict efficacy. Methods: We conducted a monocentric, observational study of mRCC patients receiving nivolumab between October 2015 and May 2020. REE was measured prior to initiating immunotherapy using indirect calorimetry to determine hypo, normo and hypermetabolism. Primary endpoint was 6-month, progression-free survival (PFS), and secondary endpoints were response rate, PFS and overall survival (OS). Results: Of the 51 consecutive patients, 15 (29%) were hypermetabolic, 24 (47%) normometabolic, and 12 (24%) hypometabolic. The 6-month PFS was 15% for hypermetabolic patients and 65% for non-hypermetabolic patients (p < 0.01). In the multivariate analysis, hypermetabolism was the only baseline factor predicting 6-month PFS (OR 9.91, 95%CI [1.62–60.55], p = 0.01). Disease progression was noted as the best response in 73% of hypermetabolic patients and 26% of non-hypermetabolic patients (p = 0.02). Median PFS was 2.8 and 8.7 months (p < 0.01), and median OS was 20.2 and 35.1 months (p = 0.13) in the hypermetabolic and non-hypermetabolic groups, respectively. Conclusions: Our study identifies an association between mRCC patients’ energy expenditure and nivolumab efficacy. The measurement of REE by indirect calorimetry in routine practice could help identify patients at risk of nivolumab failure.
Neuroendocrine Neoplasms of the Female Genitourinary Tract: A Comprehensive Overview
Authors: Virarkar Mayur , Vulasala Sai Swarupa , Gopireddy Dheeraj , Morani Ajaykumar C. , Daoud Taher , Waters Rebecca , Bhosale Priya
Published: July 2022

Primary neuroendocrine neoplasms are a rare heterogeneous group of tumors that include well-differentiated neuroendocrine tumors, poorly differentiated neuroendocrine carcinoma, and paraganglioma. An extensive literature search was used to compile the data regarding epidemiology, pathogenesis, imaging features, and management of the urinary system NENs. We also included the updated staging of the NENs at various locations of the urinary system.
Gene Therapy for Malignant and Benign Gynaecological Disorders: A Systematic Review of an Emerging Success Story
Authors: Drakopoulou Ekati , Anagnou Nicholas P. , Pappa Kalliopi I.
Published: July 2022

Despite the major advances in screening and therapeutic approaches, gynaecological malignancies still present as a leading cause of death among women of reproductive age. Cervical cancer, although largely preventable through vaccination and regular screening, remains the fourth most common and most lethal cancer type in women, while the available treatment schemes still pose a fertility threat. Ovarian cancer is associated with high morbidity rates, primarily due to lack of symptoms and high relapse rates following treatment, whereas endometrial cancer, although usually curable by surgery, it still represents a therapeutic problem. On the other hand, benign abnormalities, such as fibroids, endometriosis, placental, and embryo implantation disorders, although not life-threatening, significantly affect women’s life and fertility and have high socio-economic impacts. In the last decade, targeted gene therapy approaches toward both malignant and benign gynaecological abnormalities have led to promising results, setting the ground for successful clinical trials. The above therapeutic strategies employ both viral and non-viral systems for mutation compensation, suicide gene therapy, oncolytic virotherapy, antiangiogenesis and immunopotentiation. This review discusses all the major advances in gene therapy of gynaecological disorders and highlights the novel and potentially therapeutic perspectives associated with such an approach.
Germline Aberrations in Pancreatic Cancer: Implications for Clinical Care
Authors: Casolino Raffaella , Corbo Vincenzo , Beer Philip , Hwang Chang-il , Paiella Salvatore , Silvestri Valentina , Ottini Laura , Biankin Andrew
Published: July 2022

Pancreatic ductal adenocarcinoma (PDAC) has an extremely poor prognosis and represents a major public health issue, as both its incidence and mortality are expecting to increase steeply over the next years. Effective screening strategies are lacking, and most patients are diagnosed with unresectable disease precluding the only chance of cure. Therapeutic options for advanced disease are limited, and the treatment paradigm is still based on chemotherapy, with a few rare exceptions to targeted therapies. Germline variants in cancer susceptibility genes—particularly those involved in mechanisms of DNA repair—are emerging as promising targets for PDAC treatment and prevention. Hereditary PDAC is part of the spectrum of several syndromic disorders, and germline testing of PDAC patients has relevant implications for broad cancer prevention. Germline aberrations in BRCA1 and BRCA2 genes are predictive biomarkers of response to poly(adenosine diphosphate–ribose) polymerase (PARP) inhibitor olaparib and platinum-based chemotherapy in PDAC, while mutations in mismatch repair genes identify patients suitable for immune checkpoint inhibitors. This review provides a timely and comprehensive overview of germline aberrations in PDAC and their implications for clinical care. It also discusses the need for optimal approaches to better select patients for PARP inhibitor therapy, novel therapeutic opportunities under clinical investigation, and preclinical models for cancer susceptibility and drug discovery.
Ultra-Fast and Sensitive Screening for Antibodies against the SARS-CoV-2 S1 Spike Antigen with a Portable Bioelectric Biosensor
Authors: Mavrikou Sofia , Papaioannou George Marios , Tsekouras Vasileios , Hatziagapiou Kyriaki , Tatsi Elizabeth Barbara , Filippatos Filippos , Kanaka-Gantenbein Christina , Michos Athanasios , Kintzios Spyridon
Published: July 2022

As a consequence of the progress of the global vaccination against the COVID-19 disease, fast, accurate and affordable assays are needed for monitoring the efficiency of developing immunity against the coronavirus at the population level. In this context, we herewith report the proof-of-concept development of an innovative bioelectric biosensor for the ultra-detection (in less than three minutes) of IgG antibodies against the SARS-CoV-2 S1 spike antigen. The biosensor comprises a disposable set of screen-printed electrodes upon which are immobilized cells engineered to bear the S1 protein on their surface. When anti-S1 antibodies are presented to the engineered cell population, a rapid, specific, and selective change of the cell membrane potential occurs; this is in turn recorded by a bespoke portable potentiometer. End results are communicated via Bluetooth to a smartphone equipped with a customized user interface. By using the novel biosensor, anti-S1 antibodies could be detected at concentrations as low as 5 ng/mL. In a preliminary clinical trial, positive results were derived from patients vaccinated or previously infected by the virus. Selectivity over other respiratory viruses was demonstrated by the lack of cross-reactivity to antibodies against rhinovirus. After further clinical validation and extension to also screen IgM, IgA and possible neutralizing antibodies, our approach is intended to facilitate the mass and reliable detection of antibodies in the early stages following vaccination and to monitor the duration and level of acquired immunity both in a clinical and self-testing environment.
Effects of Treatment of Sleep Disordered Breathing on Sleep Macro- and Micro-Architecture in Children with Down Syndrome
Authors: Betavani Viecky M. P. , Davey Margot J. , Nixon Gillian M. , Walter Lisa M. , Horne Rosemary S. C.
Published: July 2022

Background: Children with Down syndrome (DS) are at increased risk of obstructive sleep disordered breathing (SDB), which is associated with intermittent hypoxia and sleep disruption affecting daytime functioning. We aimed to examine the effects of treatment of SDB on sleep quality and daytime functioning in children with DS. Methods: Children with DS and SDB (n = 24) completed a baseline and follow-up overnight polysomnographic (PSG) study 22 ± 7 months (mean ± SD) later. Sleep micro-architecture was assessed using EEG spectral analysis, and parents completed a number of questionnaires assessing sleep, behavior, daytime functioning, and quality of life (QOL). Results: A total of nine children (38%) were treated. At baseline, the treated group had more severe SDB compared to the untreated group. SDB severity was significantly improved from 40.3 ± 46.9 events/h to 17.9 ± 26.9 events/h (p < 0.01) at follow up in children who were treated. There were no significant differences in sleep macro-architecture parameters from baseline to follow up in either the treated or untreated group. Sleep micro-architecture was not different between studies in the treated group, however this tended to improve in the untreated group, particularly in REM sleep. Daytime functioning and behavior were not different between the studies in either group, however, QOL improved after treatment. Conclusions: Our study identified that treatment of SDB improves severity of the disease as defined by PSG, and this was associated with parental reports of improved QOL, despite treatment having no demonstrable impacts on sleep quality, behavior, or daytime functioning.
Impact of Time Point of Extracorporeal Membrane Oxygenation on Mortality and Morbidity in Congenital Diaphragmatic Hernia: A Single-Center Case Series
Authors: Wegele Christian , Schreiner Yannick , Perez Ortiz Alba , Hetjens Svetlana , Otto Christiane , Boettcher Michael , Schaible Thomas , Rafat Neysan
Published: July 2022

Since there are no data available on the influence of the time point of ECMO initiation on morbidity and mortality in patients with congenital diaphragmatic hernia (CDH), we investigated whether early initiation of ECMO after birth is associated with a beneficial outcome in severe forms of CDH. All neonates with CDH admitted to our institution between 2010 until 2020 and undergoing ECMO treatment were included in this study and divided into four different groups: (1) ECMO initiation < 12 h after birth (n = 143), (2) ECMO initiation between 12–24 h after birth (n = 31), (3) ECMO initiation between 24–120 h after birth (n = 48) and (4) ECMO initiation > 120 h after birth (n = 14). The mortality rate in the first (34%) and fourth group (43%) was high and in the second group (23%) and third group (12%) rather low. The morbidity, characterized by chronic lung disease (CLD), did not differ significantly in the three groups; only patients in which ECMO was initiated >120 h after birth had an increased rate of severe CLD. Our data, although not randomized and limited due to small study groups, suggest that very early need for ECMO and ECMO initiation > 120 h after birth is associated with increased mortality.
Linking Climate-Change Impacts on Hydrological Processes and Water Quality to Local Watersheds
Authors: Ouyang Ying , Panda Sudhanshu Sekhar , Feng Gary
Published: July 2022

Estimation of hydrological processes and water quality is central to water resource management, clean water supply, environmental protection, and ecological services [...]
Applying Machine Learning for Threshold Selection in Drought Early Warning System
Authors: Luo Hui , Bhardwaj Jessica , Choy Suelynn , Kuleshov Yuriy
Published: July 2022

This study investigates the relationship between the Normalized Difference Vegetation Index (NDVI) and meteorological drought category to identify NDVI thresholds that correspond to varying drought categories. The gridded evaluation was performed across a 34-year period from 1982 to 2016 on a monthly time scale for Grassland and Temperate regions in Australia. To label the drought category for each grid inside the climate zone, we use the Australian Gridded Climate Dataset (AGCD) across a 120-year period from 1900 to 2020 on a monthly scale and calculate percentiles corresponding to drought categories. The drought category classification model takes NDVI data as the input and outputs of drought categories. Then, we propose a threshold selection algorithm to distinguish the NDVI threshold to indicate the boundary between two adjacent drought categories. The performance of the drought category classification model is evaluated using the accuracy metric, and visual interpretation is performed using the heat map. The drought classification model provides a concept to evaluate drought severity, as well as the relationship between NDVI data and drought severity. The results of this study demonstrate the potential application of this concept toward early drought warning systems.
Analysis of Electrical Models for Photovoltaic Cells under Uniform and Partial Shading Conditions
Authors: Restrepo-Cuestas Bonie Johana , Durango-Flórez Mariana , Trejos-Grisales Luz Adriana , Ramos-Paja Carlos Andrés
Published: July 2022

This paper compares the performance of three electrical models (the single diode model, the Bishop model, and the Direct–Reverse model) in representing photovoltaic cells. Such comparison is performed in both the first quadrant (positive cell voltage and current—Q1) and the second quadrant (negative cell voltage and positive cell current—Q2). The analysis conducted here is based on the I–-V curves of a PV cell obtained experimentally. The parameters of each model are estimated using a Genetic Algorithm. The root mean square error and the mean absolute percentage error are computed to validate the estimation stage. Likewise, the behavior of each parameter of the models is analyzed by calculating their mean and standard deviation. Some places of interest on the I–V curve, such as the short–circuit point, the open–circuit point, and the maximum power point, are also estimated and compared.
Evaluation of Multilooking Size on Single-Baseline PolInSAR Forest Height Inversion
Authors: Wang Changcheng , Hu Chihao , Shen Peng , Song Tianyi
Published: July 2022

In polarimetric interferometric SAR (PolInSAR) technology, the random volume over ground (RVoG) model establishes the mapping relationship between polarimetric complex coherence and forest biophysical parameters (e.g., forest height). However, due to speckle noise and the finite multilooking effect, the real observed coherence region in the complex unitary circle (CUC) is an ellipse, which is biased by the ideal noise-free coherence region represented as a straight line by the RVoG model. Multilooking processing can reduce speckle noise at the cost of resolution loss. Therefore, this paper analyzes the influence of different multilooking sizes on forest height inversion. Experimental results show that the accuracy of forest height inversion first increases and then decreases with the increase in multilooking size, which means there exists an optimal size for PolInSAR forest estimation. From statistical analysis of the forest height estimation error, inversion accuracy mainly depends on estimation bias rather than estimation variance. This is mainly because, in a homogeneous forest area, a large multilooking size helps to reduce the statistical bias effect; in the textured area, the inversion accuracy benefits from a small multilooking size for avoiding the mixing of multiple types of ground targets.
A Wavelet-Based Adaptive Finite Element Method for the Stokes Problems
Authors: Mishin Yury A. , Vasilyev Oleg V. , Gerya Taras V.
Published: July 2022

In this work, we present the mathematical formulation of the new adaptive multiresolution method for the Stokes problems of highly viscous materials arising in computational geodynamics. The method is based on particle-in-cell approach—the Stokes system is solved on a static Eulerian finite element grid and material properties are carried in space by Lagrangian material points. The Eulerian grid is adapted using the wavelet-based adaptation algorithm. Both bilinear (Q1P0, Q1Q1) and biquadratic (Q2P-1) mixed approximations for the Stokes system are supported. The proposed method is illustrated for a number of linear and nonlinear two-dimensional benchmark problems of geophysical relevance. The results of the adaptive numerical simulations using the proposed method are in an excellent agreement with those obtained on non-adaptive grids and with analytical solutions, while computational requirements are few orders of magnitude less compared to the non-adaptive simulations in terms of both time and memory usage.
Protein Hydrolysates Derived from Animals and Plants—A Review of Production Methods and Antioxidant Activity
Authors: Czelej Michał , Garbacz Katarzyna , Czernecki Tomasz , Wawrzykowski Jacek , Waśko Adam
Published: July 2022

There is currently considerable interest on the use of animal, plant, and fungal sources in the production of bioactive peptides, as evidenced by the substantial body of research on the topic. Such sources provide cheap and environmentally friendly material as it often includes waste and by-products. Enzymatic hydrolysis is considered an efficient method of obtaining peptides capable of antioxidant activity. Those properties have been proven in terms of radical-scavenging capacity using the DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2-azinobis-(3-ethyl-benzothiazoline-6-sulphonic acid)), hydroxyl and superoxide radical methods. Additionally, the reducing power, ferrous ion-chelating (FIC), ferric reducing antioxidant power (FRAP), and the ability of the protein hydrolysates to inhibit lipid peroxidation have also been explored. The results collected in this review clearly indicate that the substrate properties, as well as the conditions under which the hydrolysis reaction is carried out, affect the final antioxidant potential of the obtained peptides. This is mainly due to the structural properties of the obtained compounds such as size or amino acid sequences.
Whole-Genome Sequencing of a Potential Ester-Synthesizing Bacterium Isolated from Fermented Golden Pomfret and Identification of Its Lipase Encoding Genes
Authors: Wang Huifang , Wu Yanyan , Wang Yueqi
Published: July 2022

Microbial ester synthases are regarded as valuable catalysts in the food industry. Here, one strain of Acinetobacter venetianus with ester synthase-production capacity, SCSMX-3, was isolated from traditional fermented golden pomfret. It exhibited good growth in mesophilic, low salt, and slightly alkaline environments. The ester synthase produced by SCSMX-3 displayed maximum activity at pH 8.0 and 35 °C. Genome sequencing revealed that the strain contains one circular chromosome of 336313 bp and two circular plasmids (plasmid A-14424 bp and plasmid B-11249 bp). Six CRISPR structures enhance the genomic stability of SCSMX-3 and provide the opportunity to create new functional strains. Gene function analysis indicated that SCSMX-3 produces the necessary enzymes for survival under different conditions and for flavor substance synthesis. Furthermore, 49 genes encoding enzymes associated with lipid metabolism, including three triacylglycerol lipases and two esterases, were identified through the NCBI Non-Redundant Protein Database. The lipase encoded by gene0302 belongs to the GX group and the abH15.02 (Burkholderia cepacia lipase) homolog of the abH15 superfamily. Our results shed light on the genomic diversity of and lipid metabolism in A. venetianus isolated from fermented golden pomfret, laying a foundation for the exploration of new ester synthases to improve the flavor of fermented fish products.
New Routes to Mixed “Roots”
Authors: DaCosta Kimberly
Published: July 2022

Developments in reproductive (e.g., assisted reproduction, surrogacy) and genetic technologies (commercial DNA ancestry testing) have opened new routes to mixedness that disrupt the relationship between multiracialism and family. Discussions of racial mixedness, both academic and lay, tend to refer to persons born to parents of different racialized ancestry. Multiracialism is also understood as an outcome of extended generational descent—a family lineage comprised of ancestors of varied “races”. Both modes of mixed subjectivity rely on a notion of race as transmitted through sexual reproduction, and our study of them has often focused on the implications of this boundary crossing for families. These routes to mixedness imply a degree of intimacy and “knownness” between partners, with implications for the broader web of relationships into which one is born or marries. Assisted reproduction allows for the intentional creation of mixed-race babies outside of sexual reproduction and relationship. These technologies make possible mixed race by design, in which one can choose an egg or sperm donor on the basis of their racial difference, without knowing the donor beyond a set of descriptive characteristics. Commercial DNA testing produces another route to mixedness—mixed by revelation—in which previously unknown mixed ancestry is revealed through genetic testing. Ancestry tests, however, deal in estimations of biogenetic markers, rather than specific persons. To varying degrees, these newer routes to mixedness reconfigure the nexus of biogenetic substance and kinship long foregrounded in American notions of mixedness, expand the contours of mixed-race subjectivity, and reshape notions of interracial relatedness.
Improvement of Gene Delivery and Mutation Efficiency in the CRISPR-Cas9 Wheat (Triticum aestivum L.) Genomics System via Biolistics
Authors: Tanaka Jaclyn , Minkenberg Bastian , Poddar Snigdha , Staskawicz Brian , Cho Myeong-Je
Published: July 2022

Discovery of the CRISPR-Cas9 gene editing system revolutionized the field of plant genomics. Despite advantages in the ease of designing gRNA and the low cost of the CRISPR-Cas9 system, there are still hurdles to overcome in low mutation efficiencies, specifically in hexaploid wheat. In conjunction with gene delivery and transformation frequency, the mutation efficiency bottleneck has the potential to slow down advancements in genomic editing of wheat. In this study, nine bombardment parameter combinations using three gold particle sizes and three rupture disk pressures were tested to establish optimal stable transformation frequencies in wheat. Utilizing the best transformation protocol and a knockout cassette of the phytoene desaturase gene, we subjected transformed embryos to four temperature treatments and compared mutation efficiencies. The use of 0.6 μm gold particles for bombardment increased transformation frequencies across all delivery pressures. A heat treatment of 34 °C for 24 h resulted in the highest mutation efficiency with no or minimal reduction in transformation frequency. The 34 °C treatment produced two M0 mutant events with albino phenotypes, requiring biallelic mutations in all three genomes of hexaploid wheat. Utilizing optimal transformation and heat treatment parameters greatly increases mutation efficiency and can help advance research efforts in wheat genomics.
Review of the Quality of YouTube Videos Recommending Exercises for the COVID-19 Lockdown
Authors: Rodriguez-Rodriguez Alvaro Manuel , Blanco-Diaz Maria , de la Fuente-Costa Marta , Hernandez-Sanchez Sergio , Escobio-Prieto Isabel , Casaña Jose
Published: July 2022

Background: The world is experiencing a pandemic caused by COVID-19. Insufficient physical activity can increase the risk of illness. Trying to replicate a normal search that any user/patient could do in YouTube, the objective of this study was to evaluate the quality of YouTube videos related to home exercises during lockdown and their adherence to World Health Organization (WHO) recommendations. Methods: A simple search was carried out on YouTube. The first 150 videos were selected. After applying exclusion criteria, 68 videos were analyzed and evaluated. Two statistical analyses based on machine learning techniques were carried out. Videos were classified according to principal component analysis (PCA) models as ‘Relevant’ and ‘Non-Relevant’. Popularity was assessed using the video power index (VPI). Information’s quality and accuracy were gauged using the DISCERN scale and global quality score (GQS). Reliability and credibility of information that can be found on health-related websites was assessed using the Health On the Net Code (HONCode). Exercises were evaluated according to WHO recommendations. Results: DISCERN, HONCode, and GQS scored a mean of 2.29, 58.95, and 2.32, respectively. The PCA calculation allowed videos to auto-classify into high- and low-quality videos. Conclusions: The quality of YouTube videos recommending exercises during lockdown is low and doesn’t reflect WHO recommendations. Effective strategies and tools capable of indicating the quality of this information are needed to filter out erroneous or non-rigorous information that may affect people’s health. These tools should help any user/viewer to distinguish videos of high and low quality.
Five-Year Trend of Measles and Its Associated Factors in Pahang, Malaysia: A Population-Based Study
Authors: Mat Daud Mohd Rujhan Hadfi , Yaacob Nor Azwany , Ibrahim Mohd Ismail , Wan Muhammad Wan Abdul Rahim
Published: July 2022

Measles is a disease that has resurfaced as a public health concern in Malaysia. Malaysia has had a Measles Elimination Program in place since 2004, but the incidence of measles in Pahang has not improved significantly. The purpose of this study was to describe the incidence and trend of measles in Pahang, as well as to identify the risk factors. The five-year population-based surveillance data of the entire Pahang state was extracted and analyzed for the trend and incidence of measles from January 2016 to December 2020. Multiple logistic regression was used to examine the relationship between measles and available sociodemographic data. A total of 2844 reported measles cases were investigated. Out of the measles cases reported, 7.41 percent were confirmed. According to the five-year fluctuating trend, the incidence rate ranges from 13.51 to 50.97 per 1,000,000 population. Confirmed measles was significantly associated with an indigenous background (AdjOR = 4.90, 95% CI: 1.74, 13.78), history of contact with measles cases (AdjOR = 14.03, 95% CI: 8.23, 23.90), and incomplete vaccination (AdjOR = 3.38, 95% CI: 2.28, 5.01). In conclusion, the incidence of measles in Pahang remains sporadic, owing to sporadic outbreaks. Vaccination is an important preventive measure that must reach out to the isolated populations such as indigenous people.
Disconnected: What Can We Learn from Individuals with Very Low Nature Connection?
Authors: Barrable Alexia , Booth David
Published: July 2022

While nature connection, which describes a positive relationship between humans and the rest of the natural world, has been a focus of numerous research studies in the last few decades, relatively little attention has been paid to nature disconnection. While the majority of the populations reported in most studies tend to be highly connected, there is a small percentage of those who feel they have no connection to the natural world. In this paper, we examine this novel construct of nature disconnection through secondary analysis of existing data from the Monitor of Engagement with the Natural Environment survey (MENE) by Natural England. From our analysis of this disconnected population, we can see that they are more likely to be young (16–24 years old), male, not employed and living in rented accommodation. We also observe that they have lower levels of life satisfaction and pro-environmental behaviours. We go on to present an initial theoretical discussion as to the origins of disconnection and propose further research directions to tackle the under-theorisation of this construct.
The Impact of the COVID-19 Pandemic on Healthy Lifestyle Behaviors and Perceived Mental and Physical Health of People Living with Non-Communicable Diseases: An International Cross-Sectional Survey
Authors: Azzouzi Salma , Stratton Catherine , Muñoz-Velasco Laura Paulina , Wang Kangxin , Fourtassi Maryam , Hong Bo-Young , Cooper Rory , Balikuddembe Joseph K. , Palomba Angela , Peterson Mark , Pandiyan Uma , Krassioukov Andrei , Tripathi Deo Rishi , Tuakli-Wosornu Yetsa A. , Hajjioui Abderrazak
Published: July 2022

The huge burden and vulnerability imposed by non-communicable diseases (NCDs) during the COVID-19 pandemic highlighted how healthy lifestyle behaviors and the well-being of people living with NCDs need to be prioritized. The aim of our study is to better understand the impact of the COVID-19 pandemic on healthy lifestyle behaviors and perceived mental and physical health among adults living with NCDs, as compared to people without NCDs. We conducted a cross-sectional study using a global online survey through Qualtrics. Over four months, 3550 participants from 65 countries worldwide responded to the survey. The study included 3079 surveys with no missing data (complete survey responses) that were used for analysis. People with NCDs were more likely to report statistically significant worsening physical health (p = 0.001) and statistically insignificant worsening mental health (p = 0.354) when compared to pre-pandemic levels. They reported lower rates of smoking during the pandemic than those without NCDs, and a statistically significant (p < 0.001) relationship was found between weight gain and NCDs. Therefore, the perceived physical and mental health, including changes in body weight and tobacco consumption, of people with NCDs were significantly impacted during the pandemic. In conclusion, this study indicates that the pandemic had a significant impact on perceived physical and mental health, changes in body weight, and tobacco consumption among people with NCDs.
Health-Related Content of TV and Radio Advertising of Dietary Supplements—Analysis of Legal Aspects after Introduction of Self-Regulation for Advertising of These Products in Poland
Authors: Wierzejska Regina Ewa , Wiosetek-Reske Agnieszka , Siuba-Strzelińska Magdalena , Wojda Barbara
Published: July 2022

Dietary supplements may have beneficial value but, by definition, they have no therapeutic effect. However, their labeling and especially the advertisements in the media, often make ungrounded health claims. The aim of the study was to analyze the content of audio–visual advertisements of dietary supplements for health and legal aspects in the context of the European Law and the 1 January 2020 Polish self-regulation between TV broadcasting companies and supplement manufacturers. Supplement advertisements broadcast across six TV and radio stations from 9–15 March 2020 were analyzed. Most of the analyzed advertisements complied with the legal requirements and included terms such as ‘supports’ or ‘facilitates’ body function, which are less definite in nature. Almost 30% of the advertised supplements made unproven claims on their effectiveness in various health situations, e.g., effective weight loss, thus assuring the addressee about the beneficial effect of dietary supplements in a given health context. Agreement on the rules and regulations governing supplement advertising resulted in a noticeable improvement in advertisement content, which will hopefully raise consumer awareness about the absence of therapeutic properties of dietary supplements.
The Association between the Sense of Coherence and the Self-Reported Adherence to Guidelines during the First Months of the COVID-19 Pandemic in Israel
Authors: Novak Anne Marie , Katz Adi , Bitan Michal , Lev-Ari Shahar
Published: July 2022

(1) Background: Social distancing became a central strategy employed to limit the spread of the SARS-CoV-2 virus. We explore self-reported adherence (SRA) and factors associated with SRA among Israeli adults at the end of the first national lockdown in Israel. (2) Methods: We conducted a cross-sectional consumer panel survey of 820 Israeli adults aged 18 to 70 in May and June 2020. We collected data on the SRA to the social distancing measures, sociodemographic variables, perceptions of pandemic-related danger and of protection provided by the social distancing measures, as well as Sense of Coherence (SoC). (3) Results: 60% of respondents reported complying with 7 measures. Higher SoC was associated with higher SRA (p = 0.04), and was related to income, marital status, age, profession, and education. The SRA was higher among Jews than Arabs (Jews: Mean = 10.5, SD = 4.5; Arabs: Mean = 9.1, SD = 4.1, p < 0.001) and among males (Males: Mean = 10.8, SD = 4.7; Females: Mean = 9, SD = 4.1; p = 0.003). SoC, perception of protection and perception of danger were associated with higher SRA (p = 0.42, p < 0.001 and p = 0.005 respectively). Single people reported higher levels of SRA than people in relationships (Partnered: Mean = 9.7, SD = 4.2, Non-partnered: Mean = 10.9, SD = 4.7, p = 0.033). (4) Conclusions: At the time of exit from the first lockdown, compliance with social distancing measures was high, with Jewish, single and male Israelis more likely to adhere to the guidelines. We identified the populations at risk for non-adherence and associated factors, reporting for the first time the correlation between SoC and SRA. Further research is needed to assess the role of these factors in Jewish and Arab populations.
Comparison of Wuqinxi Qigong with Stretching on Single- and Dual-Task Gait, Motor Symptoms and Quality of Life in Parkinson’s Disease: A Preliminary Randomized Control Study
Authors: Li Zhenlan , Wang Tian , Shen Mengyue , Song Tao , He Jie , Guo Wei , Wang Zhen , Zhuang Jie
Published: July 2022

The objective of this study was to investigate the therapeutic effect of Wuqinxi Qigong vs. stretching on single- and dual-task gait, motor symptoms, and quality of life in people with mild and moderate Parkinson’s disease (PD). This single-blind, randomized control trial included 40 participants with idiopathic PD who were randomized into the Wuqinxi Qigong (WQ) group or stretching group. Participants completed 12 weeks (two sessions/week) of intervention. The primary outcomes were gait parameters when performing single-task (comfortable pace) and dual-task (obstacle crossing, serial-3 subtraction and backward digit span) walking, including gait speed, stride length, and double support percentage. The secondary outcomes were ratings from the Movement Disorder Society Unified Parkinson’s Disease Rating Scale (MDS-UPDRS), results of the timed-up-and-go test (TUGT), results of the Mini-Balance Evaluation Systems Test (MiniBESTest), and responses from the 39-item Parkinson’s Disease Questionnaire (PDQ-39). All measures were assessed pre- and post-intervention. The WQ group demonstrated increased gait speed (p = 0.000) during the single task, and increased stride length (p = 0.001, p = 0.021) during the single-task and serial-3 subtraction task. Double support percentage significantly decreased (p = 0.004) in the WQ group during the obstacle crossing task, and also decreased (p = 0.045) in the stretching group during the single-task. TUGT (p = 0.005), MiniBESTest (p = 0.023) and PDQ-39 (p = 0.043) in the WQ group significantly improved, and both groups showed significant improvement in MDS-UPDRS after intervention. Wuqinxi Qigong is an effective method to improve single- and dual-task gait. While both exercises improve motor symptoms, Wuqinxi Qigong results in better mobility, balance and quality of life compared to stretching alone.
Food Safety Practices and Stunting among School-Age Children—An Observational Study Finding from an Urban Slum of Bangladesh
Authors: Sanin Kazi Istiaque , Haque Ahshanul , Nahar Baitun , Mahfuz Mustafa , Khanam Mansura , Ahmed Tahmeed
Published: July 2022

Background: Food safety incorporates the handling, preparation, and storage of food materials in ways that prevent foodborne illness. We aimed to investigate the typical food safety practices in a Bangladeshi slum context and to explore if stunting among school-age children was associated with various components of food safety. Method: We analysed the MAL-ED birth cohort data from the Bangladesh site. A total of 265 healthy children were enrolled in the study; we could follow up and collect food safety-related data from 187 participants. Results: The average age of the children was 6.5 years (standard deviation or SD 0.04) and 49% of them were female. About 26% of the children were stunted. In our bivariate analysis, caregivers’ handwashing practice after using the toilet, treatment of drinking water, presence of insects/pests in the cooking area, and child’s eating ready-made/street food more than three times per day were significantly associated with stunting. After adjusting for pertinent factors, treatment of drinking water (adjusted odds ratio or AOR = 2.50, 95% confidence interval or CI: 1.03, 6.05), and child’s eating ready-made/street food more than three times/day (AOR = 2.34, 95%CI: 1.06, 5.15) remained significantly associated with stunting. Conclusions: Diverse aspects of food safety practices have a substantial association with stunting among school-age children living in an unhygienic slum environment in Dhaka, Bangladesh.