bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantifying variations in δ18Ow and salinity in modern Bermudan waters on hourly to monthly timescales
Authors: Zhang Jade Z. , Petersen Sierra V. , Lavis Shaun , Williams Bruce
Published: August 2024

Since the mid-1970s, groundwater resources in Bermuda have been explored to supplement growing potable water demand on the island. Much of this work has focused on modeling the shape and size of freshwater lenses beneath the island’s surface, mainly the Devonshire Lens. Less attention has been paid to how these freshwater lenses interact with surrounding coastal seawater, a process that may grow in importance as sea levels rise. Due to isotopic differences between aquifer water and seawater, these interactions can be tracked using the oxygen isotopic composition of water (δ18Ow) samples collected from coastal and subterranean areas. A pilot study found more temporal variation in coastal seawater δ18Ow along Bermuda’s South Shore (the section of the coast closest to the Devonshire Lens) compared to elsewhere around the island and suggested that freshwater was discharging into coastal seawater from the Devonshire Lens in significant quantities. However, this study was limited by its small dataset so could not quantify the full spatial and temporal variability of δ18Ow in this area. Here, we present salinity and δ18Ow measurements from seawater samples collected around Bermuda and in wells tapping the Devonshire Lens on timescales ranging from hourly to monthly to better visualize the dynamic interaction between coastal seawater and aquifer-sourced freshwater. We find tight correlation between salinity and δ18Ow in well waters, indicating a simple linear mixing relationship between seawater and aquifer water in the subsurface. We confirm previous findings of larger variability in δ18Ow along the South Shore compared to elsewhere and relate observed changes to tidal height on hourly to monthly timescales. Surprisingly, South Shore seawater salinity does not vary in accordance with δ18Ow, implying additional mechanisms, such as the addition of salt spray, must be acting to mute salinity changes. These findings also demonstrate the potential in using δ18Ow to study submarine groundwater discharge, as salinity measurements alone did not detect as much variability. As sea levels rise and interactions between ocean and aquifer waters change, coastal and well water δ18Ow measurements may be helpful in tracking these processes, and in particular, changes in aquifer size.
Relationship between anterior segment biometry and primary angle-closure glaucoma in Hubei region: a retrospective case-control study
Authors: Wang Shan , Zhang Han , Yang Wanju , Zhang Ying , Qin Hui , Wang Man , Lei Xinlan , Yang Kuiliang , Zhang Hong , Xing Yiqiao
Published: August 2024

Objective

To investigate the pathogenesis of Primary Angle-Closure Glaucoma (PACG) and its relationship with the anatomical structure of the anterior segment by obtaining biometric parameters using the IOL-Master 700.

Methods

A retrospective case–control study was conducted. Clinical data from 39 PACG patients and 40 normal controls treated at the Aier Eye Hospital affiliated with Wuhan University from January to December 2022 were collected. Anterior chamber depth (AC), white-to-white (WTW), lens thickness (LT), central corneal thickness (CCT), axial length (AL), corneal curvature (K1), corneal curvature (K2), and lens position (LP) were measured using the IOL-Master 700 to analyze the characteristics and differences in the anterior segment structure of both groups. Statistical methods included independent sample t-tests and logistic regression analysis.

Results

Significant differences were found in the anterior segment biometric parameters between PACG patients and normal controls (p < 0.05). Anterior chamber depth, white-to-white, lens thickness, central corneal thickness, axial length, and K2 were all related to the occurrence of PACG (p < 0.05). The occurrence of PACG was negatively correlated with ACD, CCT, and AL (OR = 0.12–0.64, p < 0.05), and positively correlated with LT.

Conclusion

Compared to the normal control group, PACG patients in the Hubei region have a smaller anterior segment space, narrower angles, thicker lens, thinner cornea, shorter axial length, flatter cornea, and more anteriorly positioned lens.
Tolerance to induced astigmatism of patients with trifocal or extended depth of focus intraocular lens implantation
Authors: Mu Jiancheng , Xiong Tianxu , Xu Feng , Guo Wanyue , Sun Chuhuan , Chen Hao , Fan Wei
Published: August 2024

Background

Residual astigmatism is common after cataract surgery involving implantation of an intraocular lens, yet the tolerance of presbyopia-correcting intraocular lens to astigmatism of different magnitudes and axes is poorly understood. Here we compared visual acuity and quality in the presence of induced astigmatism after implantation of a trifocal or extended-depth-of-focus (EDOF) intraocular lens, the two widely used presbyopia-correcting intraocular lenses.

Methods

At least 3 months after implantation of a TFNT00 or ZXR00 intraocular lens, patients were analyzed by slit-lamp examination, non-contact tonometry, subjective refraction, iTrace aberrometry, and corneal topography. After correction of residual astigmatism, astigmatism of different magnitudes on different axes was induced using cylindrical lenses, and overall visual acuity was measured, while objective visual quality was measured using the Optical Quality Analysis System II. Subjects were also asked about subjective visual quality using the Visual Function-14 questionnaire.

Results

Comparison of 18 individuals who received a trifocal lens and 19 who received an EDOF lens showed that objective visual quality was better in the EDOF group regardless of the magnitude or axis of the induced astigmatism. In both groups, astigmatism of at least −1.00 DC influenced distant vision more severely when the axis was 45° than 0° or 90°, meanwhile astigmatism of at least −1.50 DC influenced near and intermediate vision more severely when the axis was 45° than 0° or 90°.

Conclusion

Trifocal or EDOF intraocular lenses are less tolerant of oblique astigmatism than astigmatism with or against the rule. EDOF lenses may provide better objective visual quality than trifocal lenses in the presence of astigmatism, regardless of its magnitude or axis.
Detection and counting of Leishmania intracellular parasites in microscopy images
Authors: Portuondo-Mallet Lariza María de la Caridad , Mollineda-Diogo Niurka , Orozco-Morales Rubén , Lorenzo-Ginori Juan Valentín
Published: August 2024

Problem

Leishmaniasis is a disease caused by protozoan parasites of the genus Leishmania and has a high prevalence and impact on global health. Currently, the available drugs for its treatment have drawbacks, such as high toxicity, resistance of the parasite, and high cost. Therefore, the search for new, more effective, and safe drugs is a priority. The effectiveness of an anti-leishmanial drug is analyzed through in vitro studies in which a technician manually counts the intracellular form of the parasite (amastigote) within macrophages, which is slow, laborious, and prone to errors.

Objective(s)

To develop a computational system that facilitates the detection and counting of amastigotes in microscopy images obtained from in vitro studies using image processing techniques.

Methodology

Segmentation of objects in the microscope image that might be Leishmania amastigotes was performed using the multilevel Otsu method on the saturation component of the hue, saturation, and intensity color model. In addition, morphological operations and the watershed transform combined with the weighted external distance transform were used to separate clustered objects. Then positive (amastigote) objects were detected (and consequently counted) using a classifier algorithm, the selection of which as well as the definition of the features to be used were also part of this research. MATLAB was used for the development of the system.

Results and discussion

The results were evaluated in terms of sensitivity, precision, and the F-measure and suggested a favorable effectiveness of the proposed method.

Conclusions

This system can help researchers by allowing large volumes of images of amastigotes to be counted using an automatic image analysis technique.
Expression and characterization of recombinant antibodies against H7 subtype avian influenza virus and their diagnostic potential
Authors: Wang Siwen , Zhang Ying , Zhou Xu , Ma Yue , Shi Jianzhong , Jiang Yongping , Li Yanbing , Tian Guobin , Wang Xiurong
Published: August 2024

Introduction

Monoclonal antibodies (mAbs) play a pivotal role in disease diagnosis as well as immunotherapy interventions. Traditional monoclonal antibody generation relies on animal immunization procedures predominantly involving mice; however, recent advances in in-vitro expression methodologies have enabled large-scale production suitable for both industrial applications as well as scientific investigations.

Methods

In this study, two mAbs against H7 subtype avian influenza viruses (AIV) were sequenced and analyzed, and the DNA sequences encoding heavy chain (HC) and light chain (LC) were obtained and cloned into pCHO-1.0 expression vector. Then, the HC and LC expression plasmids were transfected into CHO-S cells to establish stable cell lines expressing these mAbs using a two-phase selection scheme with different concentrations of methotrexate and puromycin. Recombinant antibodies were purified from the cell culture medium, and their potential applications were evaluated using hemagglutination inhibition (HI), western blotting (WB), confocal microscopy, and enzyme-linked immunosorbent assay (ELISA).

Results

The results indicated that the obtained recombinant antibodies exhibited biological activity similar to that of the parent antibodies derived from ascites and could be used as a replacement for animal-derived mAbs. A kinetic analysis of the two antibodies to the AIV HA protein, conducted using surface plasmon resonance (SPR), showed concordance between the recombinant and parental antibodies.

Discussion

The data presented in this study suggest that the described antibody production protocol could avoid the use of experimental animals and better conform to animal welfare regulations, and provides a basis for further research and development of mAbs-based diagnostic products.
Integrating clinical and biochemical markers: a novel nomogram for predicting lacunes in cerebral small vessel disease
Authors: Li Ning , Hu Ya-Dong , Jiang Ye , Ling Li , Wang Chu-Han , Shao Jia-Min , Li Si-Bo , Di Wei-Ying
Published: August 2024

Background

Lacunes, a characteristic feature of cerebral small vessel disease (CSVD), are critical public health concerns, especially in the aging population. Traditional neuroimaging techniques often fall short in early lacune detection, prompting the need for more precise predictive models.

Methods

In this retrospective study, 587 patients from the Neurology Department of the Affiliated Hospital of Hebei University who underwent cranial MRI were assessed. A nomogram for predicting lacune incidence was developed using LASSO regression and binary logistic regression analysis for variable selection. The nomogram’s performance was quantitatively assessed using AUC-ROC, calibration plots, and decision curve analysis (DCA) in both training (n = 412) and testing (n = 175) cohorts.

Results

Independent predictors identified included age, gender, history of stroke, carotid atherosclerosis, hypertension, creatinine, and homocysteine levels. The nomogram showed an AUC-ROC of 0.814 (95% CI: 0.791–0.870) for the training set and 0.805 (95% CI: 0.782–0.843) for the testing set. Calibration and DCA corroborated the model’s clinical value.

Conclusion

This study introduces a clinically useful nomogram, derived from binary logistic regression, that significantly enhances the prediction of lacunes in patients undergoing brain MRI for various indications, potentially advancing early diagnosis and intervention. While promising, its retrospective design and single-center context are limitations that warrant further research, including multi-center validation.
Individualized activity recommendation based on a physical fitness assessment increases short- and long-term regular physical activity in people with multiple sclerosis in a retrospective cohort study
Authors: van der Ven Eva , Patra Stefan , Riemann-Lorenz Karin , Kauschke Katrin , Freese-Schwarz Katrin , Welsch Götz , Krause Nicole , Heesen Christoph , Rosenkranz Sina Cathérine
Published: August 2024

Background

Despite the evidence of beneficial effects of physical activity (PA), people with multiple sclerosis (pwMS) are less physically active than the general population. To increase PA in pwMS, we developed a structured individually tailored PA promotion program which is conducted within clinical practice in a university-based outpatient clinic since 2016. This study serves as retrospective quality control of this program.

Objective

In a retrospective cohort study, we assessed the physical fitness of pwMS and the impact of the program on short- and long-term PA changes and behavioral determinants.

Methods

The program consisted of four appointments each 2–4 weeks apart. Spiroergometric test results of female pwMS were compared to female non-MS controls who underwent a voluntary physical fitness analysis. The short version of the Freiburger questionnaire, self-developed questions and the modified Physical activity screening questionnaire (PASQ) were sent to all participants assessing the PA levels before the program, 3 months after the program (short-term), and at the time of the survey (long-term). Additionally, established questionnaires assessed behavioral determinants before the program and long-term.

Results

A total of 166 participants [mean age 38.32 (± 10.61 SD), mean EDSS 2.30 (±1.29 SD)] and mostly females (63.3%, n = 105) were included in the study and started the program. A total of 136 participants completed the program. Out of these 63.9% (n = 87) answered the questionnaires in 12.38 (±11.34 SD) months after finishing the program. At baseline female pwMS (n = 100) showed a lower physical fitness in comparison to non-MS controls (n = 26) (maximal workload (Watts): 138.86 ± 37.85 vs. 191.73 ± 45.25, p < 0.001; peak oxygen consumption (ml min−1 kg−1): 26.40 ± 7.23 vs. 31.56 ± 10.10, p = 0.020). pwMS were more regularly active in short- (62.1%) and long-term (55.2%) compared to baseline (24.2%, p < 0.001). Among the activated participants, we observed improved internal motivation (p = 0.002) and decreased perception of barriers (p = 0.006) compared to baseline.

Conclusion

PwMS showed a lower physical fitness in comparison to non-MS controls. An individually tailored PA promotion program might improve behavioral determinants and thereby increase short- and long-term PA levels of pwMS.
Ultra-early neurological deterioration following a brain arteriovenous malformation rupture
Authors: Shotar Eimad , Chiaroni Pierre-Marie , Haffaf Idriss , Cortese Jonathan , Jacquens Alice , Garzelli Lorenzo , Allard Julien , Elhorany Mahmoud , Amouyal Caroline , Mathon Bertrand , Nouet Aurélien , Premat Kévin , Lenck Stéphanie , Sourour Nader-Antoine , Degos Vincent , Clarençon Frédéric
Published: August 2024

Purpose

This study aims to explore the impact of ultra-early neurological deterioration (U-END) on the outcome (mortality and poor neurological status) following a brain arteriovenous malformation (BAVM) rupture and identify determinants of U-END.

Methods

Patients with BAVM ruptures admitted to a single tertiary care center were retrospectively reviewed. U-END was defined as a worsening by two or more points on the Glasgow Coma Scale (GCS). U-END was tested as a potential predictor of in-hospital mortality and poor outcomes. Univariate and multivariate analyses were performed to identify determinants of U-END. Patients with U-END were also matched and compared with BAVM rupture controls presenting with a GCS close or equal to either their initial or their lowest GCS.

Results

A total of 248 patients with BAVM ruptures met the inclusion criteria, with 39 (15.7%) patients presenting with U-END. U-END was not associated with and was not an independent predictor of in-hospital mortality (12.8 vs. 10.5% in the rest of the study population; p = 0.67) or poor outcomes (39.5 vs. 36.9%; p = 0.77). The only independent determinants of U-END were hydrocephalus (OR 2.6 [95%CI, 1.1–6.4]; p = 0.03) and intraventricular hemorrhage (IVH; OR 3.5 [95%CI, 1.1–11.7]; p = 0.04). When compared to the initial GCS control group, U-END patients more often presented with IVH (89.5 vs. 64.1%; p = 0.009) and hydrocephalus (73 vs. 38.5%; p = 0.003). When compared to the lowest GCS control group, U-END patients had lower early S100B serum levels (0.35 ± 0.37 vs. 0.83 ± 1; p = 0.009) and a lower rate of poor outcome (39.5 vs. 64.9%; p = 0.03).

Conclusion

Ultra-early neurological deterioration in ruptured BAVMs did not result in increased mortality or poor outcomes and was most often related to IVH and hydrocephalus.
Oxidative stress and food as medicine
Authors: Carlson DuWayne A. , True Cheryl , Wilson Christopher G.
Published: August 2024

There has been a sea of change in our understanding of the contribution of food to both our well-being and disease states. When one addresses “food as medicine,” the concept of oxidative stress needs to be included. This review interconnects the basic science findings of oxidative stress and redox balance with the medicinal use of food, emphasizing optimization of the redox balance. To better illustrate the impacts of oxidative stress, the concept of the “triple oxidant sink” is introduced as a theoretical gauge of redox balance. Utilizing the concept, the true importance of dietary and lifestyle factors can be emphasized, including the limitations of supplements or a handful of “superfoods,” if the remainder of the factors are pro-oxidant. The effects of a whole plant food diet compared with those of dietary supplements, processed foods, animal based nutrients, or additional lifestyle factors can be visually demonstrated with this concept. This paper provides an overview of the process, acknowledging that food is not the only mechanism for balancing the redox status, but one that can be strategically used to dramatically improve the oxidative state, and thus should be used as medicine.
Renal protective and immunoregulatory effects of Lactobacillus casei strain Shirota in nephropathy-prone mice
Authors: Chan Chun-Wai , Chen Yu-Ting , Lin Bi-Fong
Published: August 2024

Introduction

The incidence of severe acute kidney injury (AKI) is considerably high worldwide. A previous study showed that gut microbial dysbiosis was a hallmark of AKI in mice. Whether the probiotic Lactobacillus casei strain Shirota (LcS) plays a role in kidney disease, particularly AKI, remains unclear.

Methods

To investigate the effects of LcS on kidney injury, tubule-specific conditional von Hippel–Lindau gene-knockout C57BL/6 mice (Vhlhdel/del mice) were supplemented without (Ctrl) or with probiotics (LcS) in Experiment 1, and their lifespan was monitored. Additionally, the Vhlhdel/+ mice were supplemented without (Ctrl and AA) or with probiotics (LcS and LcS + AA) in Experiment 2. Probiotic LcS (1 × 109 colony-forming units) was supplemented once daily. After 4 weeks of LcS supplementation, AA and LcS + AA mice were administered aristolochic acid (AA; 4 mg/kg body weight/day)-containing purified diet for 2 weeks to induce AA nephropathy before sacrifice.

Results

Supplementation of LcS significantly prolonged the lifespan of Vhlhdel/del mice, suggesting a potential renal protective effect. AA induced-nephropathy increased not only the indicators of renal dysfunction and injury, including urinary protein and kidney injury molecule (KIM)-1, serum blood urea nitrogen (BUN) and creatinine, but also serum interleukin (IL)-6 levels, renal macrophage infiltrations, and pathological lesions in Vhlhdel/+ mice. LcS supplementation significantly reduced urinary protein and KIM-1 levels, serum BUN and IL-6 levels, and renal M1 macrophages, tissue lesions, and injury scores. We also found that LcS maintained gut integrity under AA induction and increased intestinal lamina propria dendritic cells. Furthermore, LcS significantly reduced pro-inflammatory IL-17A and upregulated anti-inflammatory IL-10 production by immune cells from intestinal Peyer’s patches (PP) or mesenteric lymph nodes (MLN), and significantly increased IL-10 and reduced IL-6 production by splenocytes.

Conclusion

Prior supplementation with probiotic LcS significantly alleviated the severity of renal injury. This renal protective effect was partially associated with the enhancements of intestinal and systemic anti-inflammatory immune responses, suggesting that LcS-induced immunoregulation might contribute to its renal protective effects.
Effects of vitamin D supplementation on muscle strength in middle-aged and elderly individuals: a retrospective, propensity score-matched study
Authors: Qi Peiyao , Fu Xiaomin , Zhao Dan , Li Chunlin , Lu Yanhui , Li Nan
Published: August 2024

Introduction

The aim of this study is to investigate the impact of vitamin D supplementation on the muscle strength of the elderly.

Methods

This retrospective, propensity score-matched study included 160 middle-aged and elderly individuals from a community in Beijing, China. The control group (n=110) received health education and lifestyle guidance, while the intervention group (n=50) was given oral vitamin D supplementation in addition to health education and lifestyle guidance. All participants underwent laboratory tests, muscle function, and physical function at baseline and follow-up.

Results

In the propensity score-matched cohort of 41 patients per group, the levels of serum calcium and 25-hydroxyvitamin D in both groups were improved significantly by the end of the study (p<0.05), with the intervention group showing a more significant improvement. The muscle strength of the left lower limb in the intervention group significantly increased after the intervention (p<0.05). The results also showed that the grip strength and pinch strength of the patients in both groups increased after the intervention, and the difference between the two groups was statistically significant (p<0.05).

Discussion

The findings of this study suggest that vitamin D supplementation, in conjunction with lifestyle guidance and health education, is beneficial for enhancing the upper and lower limb strength of patients.
Josephson parametric amplifier based quantum noise limited amplifier development for axion search experiments in CAPP
Authors: Uchaikin Sergey V. , Kim Jinmyeong , Kutlu Caǧlar , Ivanov Boris I. , Kim Jinsu , van Loo Arjan F. , Nakamura Yasunobu , Ahn Saebyeok , Oh Seonjeong , Ko Minsu , Semertzidis Yannis K.
Published: August 2024

This paper provides a comprehensive overview of the development of flux-driven Josephson Parametric Amplifiers (JPAs) as Quantum Noise Limited Amplifier for axion search experiments conducted at the Center for Axion and Precision Physics Research (CAPP) of the Institute for Basic Science. It focuses on the characterization, and optimization of JPAs, which are crucial for achieving the highest sensitivity in axion particle detection. We discuss various characterization techniques, methods for improving bandwidth, and the attainment of ultra-low noise temperatures. JPAs have emerged as indispensable tools in CAPP’s axion search endeavors, playing a significant role in advancing our understanding of fundamental physics and unraveling the mysteries of the Universe.
Prevalence of anxiety and depression among patients with glaucoma
Authors: Giacometti Heloisa Helena Abil Russ , Coelho Laura Fernandes , Iankilevich Liana Grupenmacher , Valentin Livia Stocco Sanches , Ferreira Luciana Amizo , Balbino Marcos , Seixas Regina Cele Silveira
Published: August 2024

Purpose

This study aimed to determine the prevalence of anxiety and depression among patients with glaucoma compared to the average Brazilian prevalence (9.8% of anxiety and 5.8% of depression, according to the World Health Organization) and its correlation with the severity of the disease.

Methods

This was a transversal, single-arm trial of patients from four glaucoma centers in São Paulo and Curitiba—Brazil. Patients comprised adults at least 18 years of age with glaucoma diagnosis under treatment for at least 6 months. All subjects of the study answered two questionnaires (PHQ-9 and GAD-7) to evaluate the presence of anxiety and depression, and the results were analyzed accordingly to clinical and demographic characteristics.

Results

The protocol included a total of 210 patients. The average age was 61.6 ± 15.3 years, and the female gender was more common (68.86%). Primary open-angle glaucoma was the most common diagnosis (59.90%). The average IOP was 18.5 mmHg, and 1.5 anti-glaucoma drops were the mean treatment. The prevalence of depression and anxiety was 26.90 and 25.71%, respectively. Most patients with anxiety were classified as early glaucoma, while those with depression had severe glaucoma.

Conclusion

This study found that the prevalence of anxiety and depression among patients with glaucoma is higher than in the general population in our country.
Reflexivity gradient—Consciousness knowing itself
Authors: Josipovic Zoran
Published: August 2024

Some consider phenomenal consciousness to be the great achievement of the evolution of life on earth, but the real achievement is much more than mere phenomenality. The real achievement is that consciousness has woken up within us and has recognized itself, that within us humans, consciousness knows that it is conscious. This short review explores the reflexivity of consciousness from the perspective of consciousness itself—a non-conceptual nondual awareness, whose main property is its non-representational reflexivity. In light of this nondual reflexivity, different types of reflexivity proposed by current theories can be seen as a gradation of relational or transitive distances between consciousness as the knower and consciousness as the known, from fully representational and dual, through various forms of qualified monism, to fully non-representational and nondual.
Interrupted time series analysis of the impact of the COVID-19 pandemic and compulsory social health insurance system on fertility rates: a study of live births in Kazakhstan, 2019–2023
Authors: Karibayeva Indira , Moiynbayeva Sharapat , Akhmetov Valikhan , Yerkenova Sandugash , Shaikova Kuralay , Moshkalova Gaukhar , Mussayeva Dina , Tarakova Bibinur
Published: August 2024

Introduction

The COVID-19 pandemic triggered global health crises, affecting population health directly through infections and fatalities, and indirectly by increasing the burden of chronic diseases due to disrupted healthcare access and altered lifestyle behaviors. Amidst these challenges, concerns regarding reproductive health and fertility rates have emerged, necessitating an understanding of their implications for policymaking and healthcare planning. Furthermore, Kazakhstan’s healthcare landscape underwent significant changes with the reintroduction of compulsory social health insurance system in January 2020, coinciding with the onset of the COVID-19 pandemic. This study aims to assess the impact of the COVID-19 pandemic and compulsory social health insurance system on fertility rates in Kazakhstan by examining live birth data from 2019 to 2024.

Methods

Using Interrupted Time Series analysis, we evaluated the effect of the COVID-19 lockdown announcement and compulsory social health insurance system implementation on monthly birth rates, adjusted for the number of women of reproductive age from January 2019 to December 2023.

Results

In the final model, the coefficients were as follows: the effect of the COVID-19 lockdown was estimated at 469 (SE = 2600, p = 0.8576); the centering variable was estimated at 318 (SE = 222, p = 0.1573), suggesting no significant trend in monthly birth rates over time; the insurance effect was estimated at 7,050 (SE = 2,530, p < 0.01); and the effect of the number of women of reproductive age was estimated at -0.204 (SE = 0.0831, p = 0.01).

Discussion

The implementation of the compulsory social health insurance system, rather than the announcement of the COVID-19 lockdown, has had a significant positive impact on live birth rates in Kazakhstan. However, despite governmental efforts, live birth rates are declining, potentially due to unaddressed health needs of fertile women and economic challenges. Urgent policy-level actions are needed to address gaps in healthcare services and promote reproductive health.
Adolescent dysmenorrhoea in general practice: tensions and uncertainties
Authors: Dixon Sharon , Taghinejadi Neda , Duddy Claire , Holloway Flora , Vincent Katy , Ziebland Sue
Published: August 2024

This Perspectives article reflects on findings from our systematic review about adolescent dysmenorrhoea Q, drawing on sociology of diagnosis theory. We consider tensions and uncertainties between presentation with symptoms of dysmenorrhoea and processes of symptom categorisation and diagnosis in adolescents, tracing these through research and clinical guidance, considering possible implications for clinical practice. We argue that challenges in distinguishing between primary and secondary dysmenorrhoea in research translate into challenges in differentiation in clinical practice. We argue that framing this distinction as clear cut and straightforward belies the well-documented challenges in diagnosis of endometriosis, and that not recognising uncertainty and complexity inherent in this task may benefit neither clinicians nor patients.
Detecting groundwater dependence and woody vegetation restoration with NDVI and moisture trend analyses in an Indonesian karst savanna
Authors: Godwin Penelope , Tian Siyuan , Duvert Clément , Wurm Penny , Riwu Kaho Norman , Edwards Andrew
Published: August 2024

Woody vegetation restoration projects are an important feature of landscape function in Indonesian karst savannas. Understanding the relationship between available moisture and vegetation condition can assist with the planning and implementation of revegetation efforts. Working at vegetation restoration sites in East Nusa Tenggara, Indonesia, we applied a windowed cross-correlation method to mean values of NDVI to examine the lag between moisture input and NDVI response for both rainfall and soil moisture between 1999 and 2018. To test for increasing or decreasing trends in NDVI and rainfall time series, we undertook Mann–Kendall trend analyses. We identified increasing trends in Landsat 7 NDVI at two of four restoration sites, with annual increases in NDVI of 2.7 and 3.74 × 10−4 respectively. We found that rainfall dependent sites had significant Pearson’s correlations with NDVI ranging from 0.52 to 0.71, while NDVI was not correlated with rainfall at shallow groundwater sites. There was a clear negative effect of the very dry period on all sites, and this was less pronounced at shallow groundwater sites. Wet years resulted in a positive response to NDVI across all sites, while the response was lower in very wet years with annual rainfall above 1,200 mm. We found that between 2 and 4 months of antecedent rainfall gave the highest correlation with NDVI, while for soil moisture the closest relationship was found with no lag and 1 month lag. Through this study, we demonstrated the applicability of using NDVI, rainfall, and soil moisture trend analyses to identify groundwater-dependent vegetation patches and monitor the effectiveness of vegetation restoration.
Influenza in feral cat populations: insights from a study in North-East Italy
Authors: Cavicchio Lara , Campalto Mery , Carrino Marilena , Lucchese Laura , Ceglie Letizia , Fincato Alice , Boscolo Cegion Lorenza , Mazzotta Elisa , Beato Maria Serena , Natale Alda
Published: August 2024

Influenza A virus (IAV) can cause high morbidity and mortality in domestic and wild avian species and it is able to infect mammals as well. IAV in cats is sporadic and self-limiting but the recent findings of high pathogenicity avian influenza virus (HPAIV) with genetic signatures of mammalian adaptation, in domestic cats, has raised new concerns about the potential role of cats in the virus ecology. The present study aimed to investigate the circulation of IAV in companion animals' shelters in North-eastern Italy. All samples were collected from feral cats living in feline colonies that were hosted in the companion animals' shelters for the requisite period to administer the veterinary treatments. Between 2021 and 2022, 389 oropharyngeal swabs and 279 sera were collected. All swabs tested negative for IAV and the only one ELISA positive serum sample resulted H5 positive by HI test with a titer of 1:80. Despite the sporadic occurrence of influenza in cats, continuous monitoring is crucial due to the evolving zoonotic nature of the virus.
Identification and expression profiling of the bone morphogenetic protein gene family based on pearl culture in mantle and visceral mass of Hyriopsis cumingii
Authors: Chen Yiwen , Liu Shijun , Yao Yuanbin , Sun Junlin , Chen Xiaofeng , Yu Xiangyang , Xuan Xingrong , Bian Xiangli , Li Wenjuan
Published: August 2024

Bone morphogenetic proteins (BMPs) play an important biological role in pearl biomineralization in pearl mussels. In this study, based on the genome data of the triangular sail mussel (Hyriopsis cumingii), the genome-wide identification and bioinformatic analysis of BMP gene family were performed, and the expression pattern of the BMP genes was investigated by the insertion experiments. The results showed that a total of 12 BMP gene family members (BMP2a/2b, BMP3, BMP5a/5b, BMP7a/7b/7c, BMP9, BMP10a/10b, and BMP11) were identified, which were unevenly distributed on chromosome 3/14/18, encoding 169–583 amino acids, with molecular weights ranging from 19.32 to 65.99 kDa. BMP2a, BMP7b, and BMP10a were distributed, respectively, in the cytoplasm, endoplasmic reticulum and mitochondria, others were distributed in the nucleus. qRT-PCR results showed the significant tissue specificity in BMPs gene expression. The HcBMPs were differentially expressed in the mantle and visceral mass, and the expression level was higher in the visceral mass. The expressing trends of HcBMPs were not consistent between the mantle and visceral mass insertion, suggesting that HcBMPs may perform different functions. We also found that insertion surgery in the mantle and visceral mass significantly alters the expression profiling of the BMP gene family. Insertion of the mantle induced the biomineralization function of BMP2a, BMP7a, and BMP7b, while BMP3 and BMP10b played opposite roles in visceral mass insertion. Visceral mass insertion could suppress BMP9 expression at 5 d and BMP5b expression at 90 d after insertion This work lays the foundation and data support for the preliminary elucidation of regulatory role and mechanism of HcBMPs in the pearl-cultivating process of mantle and visceral mass.
Analysis of Sigma-1 Receptor Antagonist BD1047 Effect on Upregulating Proteins in HIV-1-Infected Macrophages Exposed to Cocaine Using Quantitative Proteomics
Authors: Vélez-López Omar , Carrasquillo-Carrión Kelvin , Cantres-Rosario Yadira M. , Machín-Martínez Eraysy , Álvarez-Ríos Manuel E. , Roche-Lima Abiel , Tosado-Rodríguez Eduardo L. , Meléndez Loyda M.
Published: August 2024

HIV-1 infects monocyte-derived macrophages (MDM) that migrate into the brain and secrete virus and neurotoxic molecules, including cathepsin B (CATB), causing cognitive dysfunction. Cocaine potentiates CATB secretion and neurotoxicity in HIV-infected MDM. Pretreatment with BD1047, a sigma-1 receptor antagonist, before cocaine exposure reduces HIV-1, CATB secretion, and neuronal apoptosis. We aimed to elucidate the intracellular pathways modulated by BD1047 in HIV-infected MDM exposed to cocaine. We hypothesized that the Sig1R antagonist BD1047, prior to cocaine, significantly deregulates proteins and pathways involved in HIV-1 replication and CATB secretion that lead to neurotoxicity. MDM culture lysates from HIV-1-infected women treated with BD1047 before cocaine were compared with untreated controls using TMT quantitative proteomics, bioinformatics, Lima statistics, and pathway analyses. Results demonstrate that pretreatment with BD1047 before cocaine dysregulated eighty (80) proteins when compared with the infected cocaine group. We found fifteen (15) proteins related to HIV-1 infection, CATB, and mitochondrial function. Upregulated proteins were related to oxidative phosphorylation (SLC25A-31), mitochondria (ATP5PD), ion transport (VDAC2–3), endoplasmic reticulum transport (PHB, TMED10, CANX), and cytoskeleton remodeling (TUB1A-C, ANXA1). BD1047 treatment protects HIV-1-infected MDM exposed to cocaine by upregulating proteins that reduce mitochondrial damage, ER transport, and exocytosis associated with CATB-induced neurotoxicity.
Serum Uric Acid as an Indicator of Right Ventricular Dysfunction in LVAD Patients: A Preliminary Study
Authors: Urbanowicz Tomasz , Tomaszewska Małgorzata , Olasińska-Wiśniewska Anna , Sikora Jędrzej , Straburzyńska-Migaj Ewa , Piecek Jakub , Białasik-Misiorny Maksymilian , Krasińska-Płachta Aleksandra , Tykarski Andrzej , Jemielity Marek
Published: August 2024

(1) Background: Left ventricular assist devices (LVADs) represent mechanical support in end-stage congestive heart failure and are characterized by satisfactory long-term results. Uric acid (UA) represents one of the early heart failure markers whose usefulness was postulated in clinical practice. (2) Methods: Twenty-nine male patients with a median age of 58 (51–62) years were referred for LVAD implantation due to end-stage congestive heart failure in the mean (SD) New York Heart Association (NYHA) status class 3.3 (0.6). Preoperative and postoperative right ventricular (RV) characteristics were compared with serum uric acid concentration within 12 (8–15) months following the implantation. (3) Results: Significant correlations between postoperative uric acid concentration and right ventricular dimension (r = 0.604, p = 0.005), tricuspid annulus plane systolic excursion (TAPSE) (r = −0.496, p = 0.022), left ventricular ejection fraction (r = −0.463, p = 0.046), and N-terminal pro-B-type natriuretic peptide (NT-pro-BNP) (r = 0.505, p = 0.041) were noted. (4) Conclusions: The analysis shows the association between the postoperative RV diameter and TAPSE results in LVAD patients and uric acid concentration. Serum uric acid can be regarded as a possible right ventricular dysfunction marker in LVAD patients.
Signs of Fluctuations in Energy Prices and Energy Stock-Market Volatility in Brazil and in the US
Authors: Rodrigues Gabriel Arquelau Pimenta , Serrano André Luiz Marques , Saiki Gabriela Mayumi , de Oliveira Matheus Noschang , Vergara Guilherme Fay , Fernandes Pedro Augusto Giacomelli , Gonçalves Vinícius Pereira , Neumann Clóvis
Published: August 2024

Volatility reflects the degree of variation in a time series, and a measurement of the stock performance in the energy sector can help one understand the pattern of fluctuations within this industry, as well as the factors that influence it. One of these factors could be the COVID-19 pandemic, which led to extreme volatility within the stock market in several economic sectors. It is essential to understand this regime of volatility so that robust financial strategies can be adopted to handle it. This study used stock data from the Yahoo! Finance API and data from the energy-price database from the US Energy Information Administration to conduct a comparative analysis of the volatility in the energy sector in Brazil and in the United States, as well as of the energy prices in California. The volatility in these time series were modeled using GARCH. The stock volatility regimes, both before and after COVID-19, were identified with a Markov switching model; the spillover index between the energy markets in the USA and in Brazil was evaluated with the Diebold–Yilmaz index; and the causality between the energy stock price and the energy prices was measured with the Granger causality test. The findings of this study show that (i) the volatility regime introduced by COVID-19 is still prevalent in Brazil and in the USA, (ii) the changes in the energy market in the US affect the Brazilian market significantly more than the reverse, and (iii) there is a causality relationship between the energy stock markets and the energy prices in California. These results may assist in the achievement of effective regulation and economic planning, while also supporting better market interventions. Also, acknowledging the persistent COVID-19-induced volatility can help with developing strategies for future crisis resilience.
Beyond Borders: The Effects of Immigrants on Value-Added Trade
Authors: Tadesse Bedassa , White Roger
Published: August 2024

While the effects of immigrants on aggregate trade flows have been extensively examined, the role of immigrants in shaping trade in value added (TiVA) remains underexplored. Employing a panel dataset covering 38 Organization for Economic Co-operation and Development (OECD) member host countries and 64 immigrant home countries spanning 2000–2018 and estimating a random intercept and random slope mixed-effects model, we find that immigrants play a significant role in enhancing the value added from their home countries that is embedded in their host countries’ exports to the world. We document these effects at the aggregate level and across sectors (i.e., manufacturing, agriculture, and services). There is, however, considerable variation in the influence of immigrants on TiVA across country pairs. Our findings highlight that immigrants significantly enhance trade sophistication by promoting specialization and upward movement in the value chain, yielding economic benefits for their home and host countries.
Effects of the Minimum Wage (MW) on Income Inequality: Systematic Review and Analysis of the Spanish Case
Authors: de Paz-Báñez Manuela A. , Sánchez-López Celia , Asensio-Coto María José
Published: August 2024

The minimum wage has become a standard measure in the economic and social policies of countries all over the world. The primary objective of this measure is to guarantee that workers receive a minimum wage that allows them to lead a decent life, thereby reducing inequality and poverty. However, studies on the minimum wage have not focused on assessing the effects on these dimensions but only on employment. The objective of this study is to address this research gap by analysing the effects of minimum wage increases on income inequality and poverty. To this end, firstly, a systematic review of the empirical analyses was conducted using the PRISMA methodology, with a view to ensuring that all empirical evidence was available. Secondly, the Spanish case was examined. The significant increase in minimum wage in Spain in 2019 (21.3% in real terms) presents an invaluable opportunity to utilise this event as a natural experiment to generate new evidence. A difference-in-differences approach was employed to assess the impact of this phenomenon in the period 2018–2019 with microdata from European Statistics on Income and Living Conditions (EU-SILC for Spain). In doing so, two basic scientific contributions were made. The first one, a systematic, exhaustive, and up-to-date literature review (up to June 2024), as there is, to our knowledge, no recent systematic review of this relationship (minimum wage vs. inequality). The available evidence indicates a clear inverse relationship between the minimum wage and inequalities and poverty. The second one, regarding the Spanish case, there has been a dearth of scientific studies on this subject. Thus, this paper provides new scientific evidence demonstrating that a significant increase in the minimum wage can significantly improve the income of low-wage earners, thereby reducing income inequality and in-work poverty. Furthermore, there is evidence of a spillover effect towards income groups closer to the treatment group.
Naturally Fermented Gordal and Manzanilla Green Table Olives: Effect of Single Yeast Starters on Fermentation and Final Characteristics of the Products
Authors: Ruiz-Barba José Luis , Cortés-Delgado Amparo , Sánchez Antonio Higinio , López-López Antonio , Montaño Alfredo
Published: August 2024

The influence of selected yeast starters (Kluyveromyces lactis L39, Kazachstania humilis AG5, Nakazawaea molendinolei IG9, Candida diddensiae IG12, and Candida adriatica L30) on the fermentation and final characteristics of natural-style Gordal and Manzanilla green table olives was investigated. In both cultivars, the addition of yeast starters had no significant influence on the evolution of physicochemical parameters or the final main metabolites compared to noninoculated olives. In the Gordal cultivar, K. lactis L39 originated the greatest enrichment of volatile compounds, whereas K. lactis L39 and C. adriatica L30 gave the best volatile profiles in the Manzanilla cultivar. In both cultivars, the β-glucosidase-positive strains N. molendinolei IG9, C. diddensiae IG12, and C. adriatica L30 produced no significant decrease in the total phenolic content at the end of fermentation. Although the yeast starters had a significant effect on the volatile contents of the fermented products, they did not have a significant influence on the main sensory characteristics perceived by a sensory panel. A significant linear relationship (R2 = 0.815, p < 0.001) was found and validated between the perceived bitterness intensity and the content of total phenols in olive pulp, providing a simple and objective method for the evaluation of bitterness in table olives without the need for sensory analysis.
Effects of the Toxic Dinoflagellate Protoceratium reticulatum on Physiological Rates of Juvenile Scallops Argopecten purpuratus
Authors: Nieves María Gabriela , Alvarez Gonzalo , López-Carvallo Jesús Antonio , Millanao Paulina , Araya Michael , Díaz Rosario , Díaz Patricio A.
Published: August 2024

Protoceratium reticulatum, a dinoflagellate species known for producing yessotoxins (YTX), can form harmful algal blooms (HABs) impacting marine life. This study examined how P. reticulatum influenced the physiological rates and affected the tissue health of juvenile scallops, Argopecten purpuratus. The scallops were exposed to non-toxic algae Isochrysis galbana (diet A) and mixtures where 30 and 70% of the non-toxic algae were replaced by the toxic algae P. reticulatum (diet B and C, respectively) for 15 days, followed by a 15-day recovery period (I. galbana). Results showed that the clearance rate (CR), inorganic ingestion rate (IIR), organic ingestion rate (OIR), and absorption rate (AR) were significantly reduced within the first seven days of exposure to toxic diets, with reductions of approximately 25% and 50% in diets B and C, respectively, compared to the non-toxic diet, and an increase in these parameters during the recovery stage. Histological observations revealed greater tissue damage in the digestive gland than in the gills, with a higher frequency of scallops with severe damage correlating with higher toxic diet content. Despite no direct impact on survival, the compromised physiological health of A. purpuratus juveniles suggests increased vulnerability to other stressors. These findings provide new insights into the filter-feeding behavior and selective filtration capabilities of scallops in the presence of toxic dinoflagellates and how scallops health can be compromised, contributing to the understanding of how HABs and associated toxins affect A. purpuratus.