bluuebunny/crossref_metadata_2025 huggingface dataset:
Extended Reality as an Educational Resource in the Primary School Classroom: An Interview of Drawbacks and Opportunities
Authors: Fernández-Batanero José María , Montenegro-Rueda Marta , Fernández-Cerero José , López-Meneses Eloy
Published: February 2024

The use of Extended Reality in Primary Education classrooms has emerged as a transformative element that enhances the teaching and learning process of students. In this context, examining the various effects that this tool can generate is essential to identify both the opportunities and limitations that teachers face when incorporating this technology into their practices. The aim of this research is to analyse the impact of the use of Extended Reality as an educational resource in Primary Education, focusing on teachers’ perceptions. The information was collected through semi-structured interviews with 36 active teachers in Primary Education. The analysis of the data obtained identifies the benefits and functionalities offered by the implementation of Extended Reality in Primary Education classrooms, as well as the uncertainties and concerns that teachers have with the implementation of Extended Reality. The results highlight the significant opportunities that Extended Reality offers in the teaching–learning process, provided that teachers are adequately trained. Furthermore, this study offers valuable recommendations to guide future teachers and researchers in the successful integration of this technology into the educational process.
The Role of Vegetation in Elevational Diversity Patterns of Tenebrionid Beetles in Central Italy
Authors: Fattorini Simone
Published: February 2024

Vegetation tends to vary in a systematic fashion along elevational gradients, leading to the possibility of recognizing distinct vegetational belts, which are frequently used to describe and interpret elevational variations in biodiversity. However, anthropogenic changes can create landscapes dominated by secondary grasslands in areas formerly occupied by forests, thus altering the natural sequence of vegetation types. The present research illustrates how the distribution of tenebrionid beetles in central Italy is influenced by secondary vegetation. Classical schemes of vegetational belts were modified into a scheme of main vegetation types that include secondary vegetations. Tenebrionid species presence/absence in each vegetation type was then assessed. Species richness tended to decrease with elevation in both natural and secondary vegetations. Geophilous (ground-dwelling) species (which include many endemics) prevailed in natural and secondary grasslands, while xylophilous species (associated with trees) prevailed in the natural forests. Similarities in tenebrionid composition indicated the presence of two main groups: one associated with forests and the other with natural and secondary grasslands. Geophilous species prevailed among tenebrionids with Mediterranean distributions, whereas xylophilous species prevailed among species distributed mainly in Europe and the Palearctic. High values of richness, biogeographical complexity and proportion of endemics make secondary vegetations of high conservation concern.
Ultrasound Evaluation of Sarcopenia in Patients with Hepatocellular Carcinoma: A Faster and Easier Way to Detect Patients at Risk
Authors: Esposto Giorgio , Borriello Raffaele , Galasso Linda , Termite Fabrizio , Mignini Irene , Cerrito Lucia , Ainora Maria Elena , Gasbarrini Antonio , Zocco Maria Assunta
Published: February 2024

The condition of sarcopenia, defined as a progressive loss of musculoskeletal mass and muscular strength, is very common in patients with hepatocellular carcinoma (HCC) and presents a remarkable association with its prognosis. Thus, the early identification of sarcopenic patients represents one of the potential new approaches in the global assessment of HCC, and there is increasing interest regarding the potential therapeutic implications of this condition. The gold standard for the quantification of muscle mass is magnetic resonance imaging (MRI) or computed tomography (CT), but these techniques are not always feasible because of the high-cost equipment needed. A new possibility in sarcopenia identification could be muscle ultrasound examination. The measurement of specific parameters such as the muscle thickness, muscular fascicles length or pennation angle has shown a good correlation with CT or MRI values and a good diagnostic accuracy in the detection of sarcopenia. Recently, these results were also confirmed specifically in patients with chronic liver disease. This review summarizes the role of imaging for the diagnosis of sarcopenia in patients with HCC, focusing on the advantages and disadvantages of the diagnostic techniques currently validated for this aim and the future perspectives for the identification of this condition.
The Application of Cone Beam Computed Tomography (CBCT) on the Diagnosis and Management of Maxillofacial Trauma
Authors: Rashid Arif , Feinberg Lee , Fan Kathleen
Published: February 2024

The assessment and management of facial trauma in an acute setting is one of the core services provided by oral and maxillofacial units in the United Kingdom. Imaging is a pre-requisite for appropriate diagnosis and treatment planning, with a combination of plain radiographs and medical-grade CT being the mainstay. However, the emergence of cone beam CT in recent years has led to its wider applications, including facial trauma assessment. It can offer multi-planar reformats and three-dimensional reconstruction at a much lower radiation dose and financial cost than conventional CT. The purpose of this review is to appraise its potential indications in all anatomical areas of maxillofacial trauma and provide our experience at a level 1 trauma centre.
The Evolving Classroom: How Learning Analytics Is Shaping the Future of Education and Feedback Mechanisms
Authors: Sharif Hanan , Atif Amara
Published: February 2024

In the dynamic world of higher education, technological advancements are continually reshaping teaching and learning approaches, with learning analytics (LA) playing a crucial role in this transformation. This systematic literature review (SLR) explores the significant impact of LA in higher education, specifically its transformative role in personalizing and enhancing educational feedback mechanisms. Utilizing a wide range of educational data, LA facilitates a shift from generic to individualized feedback, leading to improved learning outcomes and equity. However, incorporating LA into higher education is not without challenges, ranging from data privacy concerns to the possibility of algorithmic errors. Addressing these challenges is vital for unlocking the full potential of LA. This paper also examines emerging LA trends, such as augmented reality, emotion-sensing technology, and predictive analytics, which promise to further personalize learning experiences in higher education settings. By anchoring these advancements within core educational principles, we foresee a future of education marked by innovation and diversity. This SLR provides an overview of LA’s evolution in higher education, highlighting its transformative power, acknowledging its challenges, and anticipating its future role in shaping a dynamic, responsive educational environment.
A Review of Optical Sensors in CMOS
Authors: Gounella Rodrigo , Ferreira Gabriel M. , Amorim Marcio L. M. , Soares João Navarro , Carmo João Paulo
Published: February 2024

This paper presents an overview of silicon-based optical sensors for the measurement of light in the visible spectrum range. The review is focused on sensors based on CMOS (complementary metal-oxide semiconductor) technology due to the high availability, low cost, ease of prototyping, and well-established fabrication processes. CMOS technology allows integration with the CMOS readout and control electronics in the same microdevice, featuring high-volume fabrication with high-reproducibility and low-cost. This review starts with an explanation of the phenomena behind opto-electronic transduction. It also presents and describes the most common components responsible for optical transduction, readout electronics, and their main characteristics. This review finishes with the presentation of selected applications to grasp where and how these sensors can be used.
Techno-Economical Assessment for Combined Production of Hydrogen, Heat, and Power from Residual Lignocellulosic Agricultural Biomass in Huesca Province (Spain)
Authors: Raimondi Giulio , Greco Gianluca , Ongis Michele , D’Antuono Gabriele , Lanni Davide , Spazzafumo Giuseppe
Published: February 2024

Nowadays, great emphasis is rightly given in the scientific community to hydrogen production from electrolysis. However, to achieve the politically stated target ambitions, all low-carbon sources for hydrogen production must be considered. The present work proposes a local production system of negative carbon hydrogen from lignocellulosic residual biomass using gasification and gas separation through H2-selective membranes as enabling technologies. The feedstock is pruning. In addition, the system produces heat and power for a Renewable Energy Community (REC) to increase the economic feasibility of hydrogen production via their sale. A modular basic plant is sized, based on a simplified system envisaged for RECs under the current regulatory framework in Spain (electrical renewable output of 100 kW). A network of these modular basic plants in the province of Huesca (Aragón) is simulated to create a system of hydrogen refueling stations for mobility in that area. A Levelized Cost of Hydrogen (LCOH) is proposed, comprehending the whole production chain from “field to tank”, which is significant in areas where there is no infrastructure for the production and distribution of hydrogen for automotive purposes. The resulting LCOH for the whole system is 8.90 EUR/kg. Sensitivity analysis potentially values a lower LCOH, which unveils that hydrogen mobility can be largely competitive with diesel one.
Potential Distribution and Identification of Critical Areas for the Preservation and Recovery of Three Species of Cinchona L. (Rubiaceae) in Northeastern Peru
Authors: Coronel-Castro Elver , Meza-Mori Gerson , Torres Jose M. Camarena , Mondragón Elí Pariente , Cotrina-Sanchez Alexander , Oliva Cruz Manuel , López Rolando Salas , Campo Ramos Ricardo E.
Published: February 2024

The genus Cinchona L. has important medicinal, cultural, and economic value and is the emblematic tree of Peru. The genus is mainly found in the cloud forests of the Andes. However, the expansion of agriculture and livestock farming in the department of Amazonas is degrading these ecosystems and has reduced the size of the genus’s populations. In this work, we model the potential distribution under current conditions of three Cinchona species (C. capuli L. Anderson, C. macrocalyx Pav. Ex DC., and C. pubescens Vahl.) to identify areas with a high likelihood of species presence and their key conservation and reforestation zones. We fitted a maximum entropy (MaxEnt) model using nineteen bioclimatic variables, three topographic variables, nine edaphic variables, and solar radiation. Under current conditions, the potential distribution of C. capuli covers 17.22% (7243.98 km2); C. macrocalyx, 29.11% (12,238.91 km2); and C. pubescens, 22.94% (9647.63 km2) of the study area, which was mostly located in central and southern Amazonas. Only 24.29% (25.51% of C. capuli, 21.02% of C. macrocalyx, and 26.35% of C. pubescens) of the potential distributions are within protected areas, while 10,987.22 km2 of the surface area of the department of Amazonas is degraded, of which 29.80% covers the area of probable occurrence of C. capuli, 38.72% of C. macrocalyx, and 34.82% of C. pubescens. Consequently, it is necessary to promote additional conservation strategies for Cinchona, including the establishment of new protected areas and the recovery of degraded habitats, in order to protect this species.
Changing Dynamic of Tree Species Composition and Diversity: A Case Study of Secondary Forests in Northern China in Response to Climate Change
Authors: Liu Beichen , Liao Chengrui , Chang Youhong
Published: February 2024

Climate warming is believed to have irreversible effects on biodiversity and ecosystem functions. Secondary forests are non-negligible ecosystems in northern China that have attracted much attention because of their instability and sensitivity to global change. However, there is no consensus on the impact of warming on secondary forest succession. In this study, we explored the response of tree species diversity to climate warming in northern secondary forests in China using a series of field surveys combined with annual meteorological data from 2015 to 2021. Our results indicate that the temperature in the study area increased in spring and autumn, while the precipitation increased in spring, summer, and autumn from 2015 to 2021. Changes in species composition in the study area and climate warming were significant in the northern region of China. The importance values of many broadleaf tree species increased, whereas those of local coniferous and broadleaf tree species decreased. The Shannon–Wiener, Simpson, and Margalef indices for the pure forest were significantly lower than those for the broadleaf mixed forest and the conifer–broadleaf mixed forest (p < 0.05) in 2015 and 2021. The highest value for the Pielou index was in the conifer–broadleaf mixed forest (p < 0.05), whereas it was not significantly different between the pure forest and broadleaf mixed forest in 2021. Surprisingly, the secondary broadleaf mixed forest in northern China showed an unfavorable degradation trend under the influence of climate change, just the same as the secondary pure forest. Our work provides an experimental data source for research on secondary forests under various climate change scenarios and is an important reference for predicting and dealing with the impact of global climate change on the adaptive management and protection of secondary ecosystems.
The Impact of the Urban Forest Park Recreation Environment and Perceived Satisfaction on Post-Tour Behavioral Intention—Using Tongzhou Grand Canal Forest Park as an Example
Authors: Yao Xuhui , Sun Yan , Sun Bowen , Huang Yan
Published: February 2024

The positive behaviors that urban forest parks encourage in urban residents (such as recommendations, repeat visits, etc.) are important considerations in the planning and construction of such forest parks. Recreators’ environmental preferences and perceived satisfaction with urban forest parks are responses to individuals’ perceptions and attitudes toward the external environment. Clarifying the interactive relationship between the two is of great significance to improving positive behavioral intentions in urban forest parks. In this paper, the concept of the urban forest park recreational environment is proposed, and in this concept, the three dimensions of the spatial physical environmental preference, regional cultural characteristics, and service experience that the urban forest park recreational environment has on tourists’ perception are separated out. A model of the structural relationship between park environment perception, perceived satisfaction, and post-tour behavioral intention was tested using Tongzhou Grand Canal Forest Park as an example. The psychological path of tourists’ behavioral intention after visiting the urban forest park can be divided into two categories: the direct influencing path is “urban forest park recreation environment → post-tour behavioral intention”; the indirect influencing path is “urban forest park recreation environment → perceived satisfaction → behavioral intention after the trip”. This shows that tourists’ preference for the urban forest park recreation environment will promote the generation of perceived satisfaction and then stimulate in-depth comprehensive experience evaluation after visiting. By enhancing tourists’ experience of the urban forest park recreational environment through their environmental preference perception and improving tourists’ perceived satisfaction with the urban forest park recreational environment, urban forest parks can improve tourists’ positive behavioral intentions. Clarifying these complex psychological processes provides a new perspective for understanding people’s cognition of urban forest parks, the physiological and psychological benefits produced, and the resulting behavioral tendencies. At the same time, it also provides a new perspective for the planning and design of similar types of urban forest parks. It provides a certain reference value for forest park management.
Velocity of Money and Productivity Growth: Explaining the 2% Inflation Target in the U.S. (1959–2007)
Authors: Faugere Christophe
Published: February 2024

This article provides a macro-foundation for why the specific value of 2% is a valid inflation target. The approach postulates that innovations generate transactional cost savings by comparison to barter. The optimal velocity of money is derived as a function of productivity growth and of long-term and short-term interest rates, with coefficients reflecting the leverage ratio of depository institutions and the degree of bias in technical progress in the transaction technology. The model is tested for the U.S. (for aggregates M1, M1RS, and M1S) over the period 1959–2007. Setting the inflation target rate equal to the growth rate of velocity leads to an inflation rate near 2% and is akin to pursuing the Friedman k-% rule. This rule provides flexibility to prevent deflation. A long-term Taylor-type rule is derived. A robustness test is also conducted by extending the sample period up to 2023, covering sustained episodes of unconventional U.S. monetary policy.
Unraveling the Molecular Basis of Color Variation in Dioscorea alata Tubers: Integrated Transcriptome and Metabolomics Analysis
Authors: Wang Yue , Lu Rui-Sen , Li Ming-Han , Lu Xin-Yu , Sun Xiao-Qin , Zhang Yan-Mei
Published: February 2024

Dioscorea alata L. (Dioscoreaceae) is a widely cultivated tuber crop with variations in tuber color, offering potential value as health-promoting foods. This study focused on the comparison of D. alata tubers possessing two distinct colors, white and purple, to explore the underlying mechanisms of color variation. Flavonoids, a group of polyphenols known to influence plant color and exhibit antioxidant properties, were of particular interest. The total phenol and total flavonoid analyses revealed that purple tubers (PTs) have a significantly higher content of these metabolites than white tubers (WTs) and a higher antioxidant activity than WTs, suggesting potential health benefits of PT D. alata. The transcriptome analysis identified 108 differentially expressed genes associated with the flavonoid synthesis pathway, with 57 genes up-regulated in PTs, including CHS, CHI, DFR, FLS, F3H, F3′5′H, LAR, ANS, and ANR. The metabolomics analysis demonstrated that 424 metabolites, including 104 flavonoids and 8 tannins, accumulated differentially in PTs and WTs. Notably, five of the top ten up-regulated metabolites were flavonoids, including 6-hydroxykaempferol-7-O-glucoside, pinocembrin-7-O-(6″-O-malonyl)glucoside, 6-hydroxykaempferol-3,7,6-O-triglycoside, 6-hydroxykaempferol-7-O-triglycoside, and cyanidin-3-O-(6″-O-feruloyl)sophoroside-5-O-glucoside, with the latter being a precursor to anthocyanin synthesis. Integrating transcriptome and metabolomics data revealed that the 57 genes regulated 20 metabolites within the flavonoid synthesis pathway, potentially influencing the tubers’ color variation. The high polyphenol content and antioxidant activity of PTs indicate their suitability as nutritious and health-promoting food sources. Taken together, the findings of this study provide insights into the molecular basis of tuber color variation in D. alata and underscore the potential applications of purple tubers in the food industry and human health promotion. The findings contribute to the understanding of flavonoid biosynthesis and pigment accumulation in D. alata tubers, opening avenues for future research on enhancing the nutritional quality of D. alata cultivars.
Hydroxypropyl-Beta Cyclodextrin Barrier Prevents Respiratory Viral Infections: A Preclinical Study
Authors: Lu Angela , Ebright Brandon , Naik Aditya , Tan Hui L. , Cohen Noam A. , Bouteiller Jean-Marie C. , Lazzi Gianluca , Louie Stan G. , Humayun Mark S. , Asante Isaac
Published: February 2024

The emergence and mutation of pathogenic viruses have been occurring at an unprecedented rate in recent decades. The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has developed into a global public health crisis due to extensive viral transmission. In situ RNA mapping has revealed angiotensin-converting enzyme 2 (ACE2) expression to be highest in the nose and lower in the lung, pointing to nasal susceptibility as a predominant route for infection and the cause of subsequent pulmonary effects. By blocking viral attachment and entry at the nasal airway using a cyclodextrin-based formulation, a preventative therapy can be developed to reduce viral infection at the site of entry. Here, we assess the safety and antiviral efficacy of cyclodextrin-based formulations. From these studies, hydroxypropyl beta-cyclodextrin (HPBCD) and hydroxypropyl gamma-cyclodextrin (HPGCD) were then further evaluated for antiviral effects using SARS-CoV-2 pseudotypes. Efficacy findings were confirmed with SARS-CoV-2 Delta variant infection of Calu-3 cells and using a K18-hACE2 murine model. Intranasal pre-treatment with HPBCD-based formulations reduced viral load and inflammatory signaling in the lung. In vitro efficacy studies were further conducted using lentiviruses, murine hepatitis virus (MHV), and influenza A virus subtype H1N1. These findings suggest HPBCD may be used as an agnostic barrier against transmissible pathogens, including but not limited to SARS-CoV-2.
“Time Is out of Joint” in Pluripotent Stem Cells: How and Why
Authors: Agriesti Francesca , Cela Olga , Capitanio Nazzareno
Published: February 2024

The circadian rhythm is necessary for the homeostasis and health of living organisms. Molecular clocks interconnected by transcription/translation feedback loops exist in most cells of the body. A puzzling exemption to this, otherwise, general biological hallmark is given by the cell physiology of pluripotent stem cells (PSCs) that lack circadian oscillations gradually acquired following their in vivo programmed differentiation. This process can be nicely phenocopied following in vitro commitment and reversed during the reprogramming of somatic cells to induce PSCs. The current understanding of how and why pluripotency is “time-uncoupled” is largely incomplete. A complex picture is emerging where the circadian core clockwork is negatively regulated in PSCs at the post-transcriptional/translational, epigenetic, and other-clock-interaction levels. Moreover, non-canonical functions of circadian core-work components in the balance between pluripotency identity and metabolic-driven cell reprogramming are emerging. This review selects and discusses results of relevant recent investigations providing major insights into this context.
Resveratrol Alleviates the Early Challenges of Implant-Based Drug Delivery in a Human Glial Cell Model
Authors: Schlotterose Luise , Cossais François , Lucius Ralph , Hattermann Kirsten
Published: February 2024

Brain diseases are oftentimes life-threatening and difficult to treat. The local administration of drug substances using brain implants can increase on-site concentrations and decrease systemic side effects. However, the biocompatibility of potential brain implant materials needs to be evaluated carefully as implants can trigger foreign body reactions, particularly by increasing the microglia and astrocyte reactivity. To date, these tests have been frequently conducted in very simple in vitro models, in particular not respecting the key players in glial cell reactions and the challenges of surgical implantation characterized by the disruption of oxygen and nutrient supply. Thus, we established an in vitro model in which we treated human glial cell lines with reduced oxygen and glucose levels. The model displayed cytokine and reactive oxygen species release from reactive microglia and an increase in a marker of reactive astrocytes, galectin-3. Moreover, the treatment caused changes in the cell survival and triggered the production of hypoxia-inducible factor 1α. In this comprehensive platform, we demonstrated the protective effect of the natural polyphenol resveratrol as a model substance, which might be included in brain implants to ease the undesired glial cell response. Overall, a glial-cell-based in vitro model of the initial challenges of local brain disease treatment may prove useful for investigating new therapy options.
Susceptibility of Landslide Debris Flow in Yanghe Township Based on Multi-Source Remote Sensing Information Extraction Technology (Sichuan, China)
Authors: Guo Hongyi , Martínez-Graña A. M.
Published: February 2024

The extraction of real geological environment information is a key factor in accurately evaluating the vulnerability to geological hazards. Yanghe Township is located in the mountainous area of western Sichuan and lacks geological survey data. Therefore, it is important predict the spatial and temporal development law of landslide debris flow in this area and improve the effectiveness and accuracy of monitoring changes in landslide debris flow, this article proposes a method for extracting information on the changes in landslide debris flows combined with NDVI variation, which is based on short baseline interferometry (SBAS-InSAR) and optical remote sensing interpretation. In this article, we present relevant maps based on six main factors: vegetation index, slope, slope orientation, elevation, topographic relief, and formation lithology. At the same time, different remote sensing images were compared to improve the accuracy of landslide debris flow sensitivity assessments. The research showed that the highest altitude of the region extracted by multi-source remote sensing technology is 2877 m, and the lowest is 630 m, which can truly reflect the topographic relief characteristics of the region. The pixel binary model’s lack of regional restrictions enables a more accurate estimation of the Normalized Difference Vegetation Index (NDVI), bringing it closer to the actual vegetation situation. The study uncovered a bidirectional relationship between vegetation coverage changes and landslide deformation in the study area, revealing spatial–temporal evolution patterns. By employing multi-source remote sensing technology, the research effectively utilized changes in multi-period imagery and feature extraction methods to accurately depict the development process and distribution characteristics of landslide debris flow. This approach not only offers technical support but also provides guidance for evaluating the vulnerability of landslide debris flow in the region.
Influence of Non-Parallelism on the Micro-Interface Lubrication Mechanism of Water-Lubricated Bearings
Authors: Sun Lin , Shi Jianchao , Jiang Tao , Li Zhen , Wang Yu , Liu Zhaozeng
Published: February 2024

Water-lubricated bearings can effectively solve the pollution problem caused by lubricant leakage and are used in offshore engineering equipment for this reason. Aiming at the problems of unclear and undefined micro-interface lubrication mechanisms of water-lubricated bearings, this paper investigates the influence of non-parallel micro-cavities on the micro-interface lubrication mechanism of bearings. Based on a single micro-cavity model, the lubrication mechanism of micro-cavities is studied in this paper. Lubrication models of the non-parallel contact friction pairs model are built, and the effect of the non-parallelism on the lubrication performance of the micro-cavities is obtained using the computational fluid dynamics method. The results show that, under the same Reynolds number and cavitation pressure, the wedge effect caused by the non-parallelism causes the pressure at the inlet to rise, thus increasing the load-carrying capacity. The existence of non-parallelism limits the rise of the high pressure of the inertia effect on the micro-cavities and reduces the load-carrying capacity. The presence of non-parallelism decreases the area of the negative pressure proportion and increases the proportion of the positive pressure zone inside the micro-cavities, thus increasing the load-carrying capacity.
An Integrated Framework for Image Acquisition, Processing, and Analysis Procedures for Automated Damage Evaluation of Concrete Surfaces
Authors: Zhang Haixu , Trottier Cassandra , Sanchez Leandro F. M. , Allard Anthony
Published: February 2024

Concrete surface cracks serve as early indicators of potential structural threats. Visual inspection, a commonly used and versatile concrete condition assessment technique, is employed to assess concrete degradation by observing signs of damage on the surface level. However, the method tends to be qualitative and needs to be more comprehensive in providing accurate information regarding the extent of damage and its evolution, notwithstanding its time-consuming and environment-sensitive nature. As such, the integration of image analysis techniques with artificial intelligence (AI) has been increasingly proven efficient as a tool to capture damage signs on concrete surfaces. However, to improve the performance of automated crack detection, it is imperative to intensively train a machine learning model, and questions remain regarding the required image quality and image collection methodology needed to ensure the model’s accuracy and reliability in damage quantitative analysis. This study aims to establish a procedure for image acquisition and processing through the application of an image-based measurement approach to explore the capabilities of concrete surface damage diagnosis. Digitizing crack intensity measurements were found to be feasible; however, larger datasets are required. Due to the anisotropic behavior of the damage, the model’s ability to capture crack directionality was developed, presenting no statistically significant differences between the observed and predicted values used in this study with correlation coefficients of 0.79 and 0.82.
Applied Mathematics in the Numerical Modelling of the Electromagnetic Field in Reference to Drying Dielectrics in the RF Field
Authors: Spoiala Viorica , Silaghi Helga , Spoiala Dragos
Published: February 2024

The processing of dielectric materials in the radio frequency field continues to be a concern in engineering. This procedure involves a rigorous analysis of the electromagnetic field based on specific numerical methods. This paper presents an original method for analysing the process of drying wooden boards in a radio frequency (RF) installation. The electromagnetic field and thermal field are calculated using the finite element method (FEM). The load capacity of the installation is also calculated, since the material being heated in the radio frequency heating installations is placed in a capacitor-type applicator. A specific method is created in order to solve the problem related to mass, a quantity which tends to change during the drying of the dielectric. In addition, special consideration is given to issues regarding the coupling of the electromagnetic field and the thermal field, along with aspects pertaining to mass. These are implemented numerically using a program written in the Fortran language, which takes the distribution of finite elements from the Flux2D program, the dielectric thermal module, intended only for the study of RF heating. The results obtained after running the program are satisfactory and they represent a support for future studies, especially if the movement of the dielectric is taken into account.
Effect of Graphene Oxide as an Anodizing Additive for the ZK60A Magnesium Alloy: Correlating Corrosion Resistance, Surface Chemistry and Film Morphology
Authors: Braga Paula Lima , de Souza Denise Criado Pereira , de Oliveira Mara Cristina Lopes , Antunes Renato Altobelli
Published: February 2024

The aim of the present work was to study the effect of graphene oxide as an additive in the anodization bath of the ZK60A magnesium alloy on the corrosion resistance, film morphology and surface chemical composition. The anodizing process was conducted at a constant current density of 30 mA.cm−2 in an electrolyte consisting of 3 M de KOH, 0.15 M de Na2SiO3 and 0.1 M Na2B4O7.10H2O. Graphene oxide was added to this bath at three different concentrations: 0.5 g.L−1, 1.0 g.L−1 and 3.0 g.L−1. The ability of the graphene oxide nanofiller to enhance the corrosion resistance of the ZK60A alloy was evaluated by electrochemical impedance spectroscopy and potentiodynamic polarization tests in 3.5 wt.% NaCl solution. The surface chemical composition was assessed by X-ray photoelectron spectroscopy (XPS). Scanning electron microscopy (SEM) coupled with EDS analysis was employed to examine the anodized layer morphology and thickness. The results pointed to a beneficial effect of graphene oxide addition on the corrosion resistance of the anodized ZK60A which was dependent on the concentration of the nanofiller in the anodizing electrolyte.
UHPLC-MS/MS Assay for Quantification of Legubicin, a Novel Doxorubicin-Based Legumain-Activated Prodrug, and Its Application to Pharmacokinetic and Tissue Distribution Studies
Authors: Ma Liyuan , Yu Qiaoling , Zhuang Meng , Yang Chen , Liu Yuan , Li Yuling , Liu Cheng , Shen Xiaoyan , Chang Yan
Published: February 2024

Legubicin, a novel prodrug based on doxorubicin, has both albumin-binding and legumain-activating properties. The aim of this study was to develop and validate a UHPLC-MS/MS method for investigating the in vivo pharmacokinetics and tissue distribution profiles of legubicin in rats and tumor-bearing mice following intravenous administration, and to compare this prodrug with the positive control drug doxorubicin. The study employed a UHLC-MS/MS method to determine the levels of albumin-bound of legubicin and two metabolites (free Leu-DOX and DOX) in plasma, tumor, and tissue samples. This method was validated for good selectivity, high sensitivity, excellent extraction recovery, and short run time. The results showed that legubicin was present in the circulation in vivo mainly in a protein-bound form with larger AUC values and lower clearance and distribution, and essentially released small amounts of doxorubicin. Compared to administration of equimolar doses of doxorubicin, legubicin showed increased exposure of the active drug in the tumor and decreased the level of the active drug in the heart and kidney. This study provides valuable information on the pharmacokinetics and tissue distribution of legubicin, implicating its potential as a novel and effective drug candidate for anti-cancer therapies.
Comparison of Multiple NIR Spectrometers for Detecting Low-Concentration Nitrogen-Based Adulteration in Protein Powders
Authors: Lukacs Matyas , Zaukuu John-Lewis Zinia , Bazar George , Pollner Bernhard , Fodor Marietta , Kovacs Zoltan
Published: February 2024

Protein adulteration is a common fraud in the food industry due to the high price of protein sources and their limited availability. Total nitrogen determination is the standard analytical technique for quality control, which is incapable of distinguishing between protein nitrogen and nitrogen from non-protein sources. Three benchtops and one handheld near-infrared spectrometer (NIRS) with different signal processing techniques (grating, Fourier transform, and MEM—micro-electro-mechanical system) were compared with detect adulteration in protein powders at low concentration levels. Whey, beef, and pea protein powders were mixed with a different combination and concentration of high nitrogen content compounds—namely melamine, urea, taurine, and glycine—resulting in a total of 819 samples. NIRS, combined with chemometric tools and various spectral preprocessing techniques, was used to predict adulterant concentrations, while the limit of detection (LOD) and limit of quantification (LOQ) were also assessed to further evaluate instrument performance. Out of all devices and measurement methods compared, the most accurate predictive models were built based on the dataset acquired with a grating benchtop spectrophotometer, reaching R2P values of 0.96 and proximating the 0.1% LOD for melamine and urea. Results imply the possibility of using NIRS combined with chemometrics as a generalized quality control tool for protein powders.
Stabilizing Highly Active Ru Sites by Electron Reservoir in Acidic Oxygen Evolution
Authors: Wu Jiayan , Qiu Zhongjie , Zhang Jiaxi , Song Huiyu , Cui Zhiming , Du Li
Published: February 2024

Proton exchange membrane water electrolysis is hindered by the sluggish kinetics of the anodic oxygen evolution reaction. RuO2 is regarded as a promising alternative to IrO2 for the anode catalyst of proton exchange membrane water electrolyzers due to its superior activity and relatively lower cost compared to IrO2. However, the dissolution of Ru induced by its overoxidation under acidic oxygen evolution reaction (OER) conditions greatly hinders its durability. Herein, we developed a strategy for stabilizing RuO2 in acidic OER by the incorporation of high-valence metals with suitable ionic electronegativity. A molten salt method was employed to synthesize a series of high-valence metal-substituted RuO2 with large specific surface areas. The experimental results revealed that a high content of surface Ru4+ species promoted the OER intrinsic activity of high-valence doped RuO2. It was found that there was a linear relationship between the ratio of surface Ru4+/Ru3+ species and the ionic electronegativity of the dopant metals. By regulating the ratio of surface Ru4+/Ru3+ species, incorporating Re, with the highest ionic electronegativity, endowed Re0.1Ru0.9O2 with exceptional OER activity, exhibiting a low overpotential of 199 mV to reach 10 mA cm−2. More importantly, Re0.1Ru0.9O2 demonstrated outstanding stability at both 10 mA cm−2 (over 300 h) and 100 mA cm−2 (over 25 h). The characterization of post-stability Re0.1Ru0.9O2 revealed that Re promoted electron transfer to Ru, serving as an electron reservoir to mitigate excessive oxidation of Ru sites during the OER process and thus enhancing OER stability. We conclude that Re, with the highest ionic electronegativity, attracted a mass of electrons from Ru in the pre-catalyst and replenished electrons to Ru under the operating potential. This work spotlights an effective strategy for stabilizing cost-effective Ru-based catalysts for acidic OER.
Non-Volatile Component and Antioxidant Activity: A Comparative Analysis between Litsea cubeba Branches and Leaves
Authors: Dai Wei , Li Boyi , Xiong Yanli , Dai Liping , Tian Yuan , Zhang Liangqian , Wang Qi , Qian Guoqiang
Published: February 2024

Litsea cubeba, which is found widely distributed across the Asian region, functions as both an economic tree and a medicinal plant with a rich historical background. Previous investigations into its chemical composition and biological activity have predominantly centered on volatile components, leaving the study of non-volatile components relatively unexplored. In this study, we employed UPLC-HRMS technology to analyze the non-volatile components of L. cubeba branches and leaves, which successfully resulted in identifying 72 constituents. Comparative analysis between branches and leaves unveiled alkaloids, organic acids, and flavonoids as the major components. However, noteworthy differences in the distribution of these components between branches and leaves were observed, with only eight shared constituents, indicating substantial chemical variations in different parts of L. cubeba. Particularly, 24 compounds were identified for the first time from this plant. The assessment of antioxidant activity using four methods (ABTS, DPPH, FRAP, and CUPRAC) demonstrated remarkable antioxidant capabilities in both branches and leaves, with slightly higher efficacy observed in branches. This suggests that L. cubeba may act as a potential natural antioxidant with applications in health and therapeutic interventions. In conclusion, the chemical composition and antioxidant activity of L. cubeba provides a scientific foundation for its development and utilization in medicine and health products, offering promising avenues for the rational exploitation of L. cubeba resources in the future.
Unveiling Epigenetic Vulnerabilities in Triple-Negative Breast Cancer through 3D Organoid Drug Screening
Authors: Rao Xinxin , Qiao Zhibin , Yang Yang , Deng Yun , Zhang Zhen , Yu Xiaoli , Guo Xiaomao
Published: February 2024

Triple-negative breast cancer (TNBC) poses a therapeutic challenge due to its aggressive nature and lack of targeted therapies. Epigenetic modifications contribute to TNBC tumorigenesis and drug resistance, offering potential therapeutic targets. Recent advancements in three-dimensional (3D) organoid cultures, enabling precise drug screening, hold immense promise for identifying novel compounds targeting TNBC. In this study, we established two patient-derived TNBC organoids and implemented a high-throughput drug screening system using these organoids and two TNBC cell lines. Screening a library of 169 epigenetic compounds, we found that organoid-based systems offer remarkable precision in drug response assessment compared to cell-based models. The top 30 compounds showing the highest drug sensitivity in the initial screening were further assessed in a secondary screen. Four compounds, panobinostat, pacritinib, TAK-901, and JIB-04, targeting histone deacetylase, JAK/STAT, histone demethylases, and aurora kinase pathways, respectively, exhibited potent anti-tumor activity in TNBC organoids, surpassing the effect of paclitaxel. Our study highlights the potential of these novel epigenetic drugs as effective therapeutic agents for TNBC and demonstrates the valuable role of patient-derived organoids in advancing drug discovery.
Chemical Profiling, Insecticidal, and Phytotoxic Effect of Essential Oils from Leaves and Inflorescence of Moroccan Chenopodium ambrosioides (L.)
Authors: Kasrati Ayoub , Sakar El Hassan , Aljaiyash Ahmed , Hirri Aziz , Tamegart Lahcen , Abbad Imane , Alaoui Jamali Chaima
Published: February 2024

Chenopodium ambrosioides (L.) is a medicinal and aromatic plant widely used in the Moroccan traditional medicine for its important pharmacological properties. In order to contribute to the valorization of this plant, the current study aimed at evaluating, for the first time, the variation in the yield, chemical composition, and insecticidal and phytotoxic activities of C. ambrosioides essential oil (CAEO) isolated from leaves and inflorescences. The results obtained showed that the CAEO yields vary significantly according to the distilled plant part, with 0.75 ± 0.15% for the leaves and 1.2 ± 0.34% for the inflorescences. CAEO profiling showed quantitative more than qualitative differences. Leaf CAEO was rich in δ-3-carene (61.51%), followed by p-cymene (14.67%) and 1,2:3,4-diepoxy-p-menthane (6.19%). However, inflorescence CAEO was dominated by the same compounds but with variable levels (δ-3-Carene: 44.29%; 1,2:3,4-diepoxy-p-menthane: 19.46%; and p-cymene: 17.85%). The CAEOs from the leaves and inflorescences showed a very interesting inhibiting effect on the germination and growth of the three species used, namely Medicago sativa, Linum rusitatissimum, and Raphanus sativus. However, there was no significant difference between these CAEOs. Similarly, for the insecticidal activity, CAEOs exhibited an important and similar toxicity against Tribolium confusum adults with LD50 of 4.30 and 4.46 μL/L of air and LD90 of 6.51 and 9.62 μL/L air for toxicity by fumigation, while values for contact toxicity on filter paper were 0.04 and 0.05 μL/cm2 for LD50 and 0.08 and 0.09 μL/cm2 for LD90.
Cloning, Expression, and Functional Analysis of the MYB Transcription Factor SlMYB86-like in Tomato
Authors: Chen Na , Zhan Wenwen , Shao Qin , Liu Liangliang , Lu Qineng , Yang Weihai , Que Zhiqun
Published: February 2024

MYB transcription factors (TFs) have been shown to play a key role in plant growth and development and are in response to various types of biotic and abiotic stress. Here, we clarified the structure, expression patterns, and function of a MYB TF, SlMYB86-like (Solyc06g071690) in tomato using an inbred tomato line exhibiting high resistance to bacterial wilt (Hm 2-2 (R)) and one susceptible line (BY 1-2 (S)). The full-length cDNA sequence of this gene was 1226 bp, and the open reading frame was 966 bp, which encoded 321 amino acids; its relative molecular weight was 37.05055 kDa; its theoretical isoelectric point was 7.22; it was a hydrophilic nonsecreted protein; and it had no transmembrane structures. The protein also contains a highly conserved MYB DNA-binding domain and was predicted to be localized to the nucleus. Phylogenetic analysis revealed that SlMYB86-like is closely related to SpMYB86-like in Solanum pennellii and clustered with other members of the family Solanaceae. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of the SlMYB86-like gene was tissue specific and could be induced by Ralstonia solanacearum, salicylic acid, and jasmonic acid. The results of virus-induced gene silencing (VIGS) revealed that SlMYB86-like silencing decreased the resistance of tomato plants to bacterial wilt, suggesting that it positively regulates the resistance of tomatoes to bacterial wilt. Overall, these findings indicate that SlMYB86-like plays a key role in regulating the resistance of tomatoes to bacterial wilt.
Climate Change—The Rise of Climate-Resilient Crops
Authors: Kopeć Przemysław
Published: February 2024

Climate change disrupts food production in many regions of the world. The accompanying extreme weather events, such as droughts, floods, heat waves, and cold snaps, pose threats to crops. The concentration of carbon dioxide also increases in the atmosphere. The United Nations is implementing the climate-smart agriculture initiative to ensure food security. An element of this project involves the breeding of climate-resilient crops or plant cultivars with enhanced resistance to unfavorable environmental conditions. Modern agriculture, which is currently homogeneous, needs to diversify the species and cultivars of cultivated plants. Plant breeding programs should extensively incorporate new molecular technologies, supported by the development of field phenotyping techniques. Breeders should closely cooperate with scientists from various fields of science.
The Universal Light, or the Only Way to the Father? Universalism and Exclusivism in John’s Provocative Christology
Authors: Anderson Paul N.
Published: February 2024

Among the most perplexing of John’s theological riddles is question of salvific universalism and particularity. John is both the greatest biblical source of Christian universalism and the greatest source of Christian exclusivity. After all, the Johannine Overture affirms universal access to the saving–revealing Light of Christ, declaring: “The true light, which enlightens everyone, was coming into the world!” (Jn 1:9). What else could this mean, other than to affirm that every person has saving access to the divine Light of Christ? And yet, Jesus also declares in John 14:6, “I am the way, and the truth, and the life. No one comes to the Father except through me!” What could this mean, other than to assert that there is only one means of access to God, and that it involves saving belief in Jesus as the Christ? Sorting out the apparent contradictions within these tensions is the goal of the present essay.