bluuebunny/crossref_metadata_2025 huggingface dataset:
Publisher Correction: Hyperpolarized [1-13C]-acetate Renal Metabolic Clearance Rate Mapping
Authors: Mikkelsen Emmeli F. R. , Mariager Christian Østergaard , Nørlinger Thomas , Qi Haiyun , Schulte Rolf F. , Jakobsen Steen , Frøkiær Jørgen , Pedersen Michael , Stødkilde-Jørgensen Hans , Laustsen Christoffer
Published: July 2018

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
PD-L1 expression in pleural effusions of pulmonary adenocarcinoma and survival prediction: a controlled study by pleural biopsy
Authors: Xu Jian , Han Xue , Liu Chunfang , Gao Na , Zhao Junjun , Zhang Xiaolin , Jiang Ling , Ren Lina , Li Ping , Wang Nini
Published: July 2018

PD-L1 expression in pleural effusions (PE) of lung adenocarcinoma (ADC) was compared with pleural biopsies and the positive expression in PE was correlated with survival time. The matched slices from same patient’s pleura and PE were collected which both were pathologically verified positive. Immunohistochemistry (IHC) was used to detect PD-L1 expression. A total of 51 eligible cases were enrolled, including 30 males and 21 females. The average age was (67.06 ± 12.10) years. PD-L1 expression wasn’t statistically significant in pleura and cell masses (P > 0.05) and the correlation was statistically significant (r = 0.585, P = 0.000). Using an IHC scores of 5 point as a cutoff, positive PD-L1 expression in the pleura was 11.63% and that in the cell masses was 23.26%, and difference was significant (P < 0.05). The correlation coefficient was 0.605. Among 35 cases underwent systemic anti-tumor treatment, the mean survival time with positive PD-L1 expression in PE was 17.370 ± 1.827 months, which was significantly shorter than that with the negative (29.944 ± 2.671 months) (χ2 = 4.507, P = 0.034). Positive PD-L1 expression in PE is higher than in the pleura and their correlation is well. It may predict the short survival time after systemic anti-tumor treatment.
Periostin contributes to the maturation and shape retention of tissue-engineered cartilage
Authors: Inaki Ryoko , Fujihara Yuko , Kudo Akira , Misawa Masaki , Hikita Atsuhiko , Takato Tsuyoshi , Hoshi Kazuto
Published: July 2018

Traditional tissue-engineered cartilage applied in clinical practice consists of cell suspensions or gel-form materials for which it is difficult to maintain their shapes. Although biodegradable polymer scaffolds are used for shape retention, deformation after transplantation can occur. Here, we showed that periostin (PN), which is abundantly expressed in fibrous tissues, contributes to the maturation and shape retention of tissue-engineered cartilage through conformational changes in collagen molecules. The tissue-engineered cartilage transplanted in an environment lacking PN exhibited irregular shapes, while transplants originating from chondrocytes lacking PN showed limited regeneration. In thein vitroassay, PN added to the culture medium of chondrocytes failed to show any effects, while the 3D culture embedded within the collagen gel premixed with PN (10 μg/mL) enhanced chondrogenesis. The PN-mediated collagen structure enhanced the mechanical strength of the surrounding fibrous tissues and activated chondrocyte extracellular signaling by interstitial fibrous tissues.
Author Correction: Multivariate EEG analyses support high-resolution tracking of feature-based attentional selection
Authors: Fahrenfort Johannes Jacobus , Grubert Anna , Olivers Christian N. L. , Eimer Martin
Published: July 2018

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
Overexpression of Tisochrysis lutea Akd1 identifies a key cold-induced alkenone desaturase enzyme
Authors: Endo Hirotoshi , Hanawa Yutaka , Araie Hiroya , Suzuki Iwane , Shiraiwa Yoshihiro
Published: July 2018

Alkenones are unusual long-chain neutral lipids that were first identified in oceanic sediments. Currently they are regarded as reliable palaeothermometers, since their unsaturation status changes depending on temperature. These molecules are synthesised by specific haptophyte algae and are stored in the lipid body as the main energy storage molecules. However, the molecular mechanisms that regulate the alkenone biosynthetic pathway, especially the low temperature-dependent desaturation reaction, have not been elucidated. Here, using an alkenone-producing haptophyte alga, Tisochrysis lutea, we show that the alkenone desaturation reaction is catalysed by a newly identified desaturase. We first isolated two candidate desaturase genes and found that one of these genes was drastically upregulated in response to cold stress. Gas chromatographic analysis revealed that the overexpression of this gene, named as Akd1 finally, increased the conversion of di-unsaturated C37-alkenone to tri-unsaturated molecule by alkenone desaturation, even at a high temperature when endogenous desaturation is efficiently suppressed. We anticipate that the Akd1 gene will be of great help for elucidating more detailed mechanisms of temperature response of alkenone desaturation, and identification of active species contributing alkenone production in metagenomic and/or metatranscriptomic studies in the field of oceanic biogeochemistry.
Analysis of public RNA-sequencing data reveals biological consequences of genetic heterogeneity in cell line populations
Authors: Fasterius Erik , Al-Khalili Szigyarto Cristina
Published: July 2018

Meta-analysis of datasets available in public repositories are used to gather and summarise experiments performed across laboratories, as well as to explore consistency of scientific findings. As data quality and biological equivalency across samples may obscure such analyses and consequently their conclusions, we investigated the comparability of 85 public RNA-seq cell line datasets. Thousands of pairwise comparisons of single nucleotide variants in 139 samples revealed variable genetic heterogeneity of the eight cell line populations analysed as well as variable data quality. The H9 and HCT116 cell lines were found to be remarkably stable across laboratories (with median concordances of 99.2% and 98.5%, respectively), in contrast to the highly variable HeLa cells (89.3%). We show that the genetic heterogeneity encountered greatly affects gene expression between same-cell comparisons, highlighting the importance of interrogating the biological equivalency of samples when comparing experimental datasets. Both the number of differentially expressed genes and the expression levels negatively correlate with the genetic heterogeneity. Finally, we demonstrate how comparing genetically heterogeneous datasets affect gene expression analyses and that high dissimilarity between same-cell datasets alters the expression of more than 300 cancer-related genes, which are often the focus of studies using cell lines.
KampoDB, database of predicted targets and functional annotations of natural medicines
Authors: Sawada Ryusuke , Iwata Michio , Umezaki Masahito , Usui Yoshihiko , Kobayashi Toshikazu , Kubono Takaki , Hayashi Shusaku , Kadowaki Makoto , Yamanishi Yoshihiro
Published: July 2018

Natural medicines (i.e., herbal medicines, traditional formulas) are useful for treatment of multifactorial and chronic diseases. Here, we present KampoDB (http://wakanmoview.inm.u-toyama.ac.jp/kampo/), a novel platform for the analysis of natural medicines, which provides various useful scientific resources on Japanese traditional formulas Kampo medicines, constituent herbal drugs, constituent compounds, and target proteins of these constituent compounds. Potential target proteins of these constituent compounds were predicted by docking simulations and machine learning methods based on large-scale omics data (e.g., genome, proteome, metabolome, interactome). The current version of KampoDB contains 42 Kampo medicines, 54 crude drugs, 1230 constituent compounds, 460 known target proteins, and 1369 potential target proteins, and has functional annotations for biological pathways and molecular functions. KampoDB is useful for mode-of-action analysis of natural medicines and prediction of new indications for a wide range of diseases.
Relationship between Anaemia, Haemolysis, Inflammation and Haem Oxygenase-1 at Admission with Sepsis: a pilot study
Authors: Ekregbesi Phebe , Shankar-Hari Manu , Bottomley Christian , Riley Eleanor M. , Mooney Jason P.
Published: July 2018

Upregulation of haem oxygenase-1 (HO-1), due to haemolysis and/or inflammation, can lead to impaired immune function. Anaemia is common among sepsis patients, but the consequences of sepsis-associated anaemia are poorly understood. Here, our objective was to determine the prevalence and extent of anaemia, haemolysis, inflammation, and HO-1 induction after early hospital admission. We hypothesised that inflammation- or infection-induced haemolysis contributes to sepsis-associated anaemia and that this will lead to expression of HO-1. In this study, plasma obtained from seventy adult patients within 12 hours of admission to intensive care due to sepsis were analysed for anaemia, haemolysis and inflammatory markers by ELISA and microbead array. The majority (82.6%) of patients were anaemic with evidence of haemolysis (raised haem, haptoglobin, haemopexin, and HO-1 concentrations). Interestingly, concentrations of both haemoglobin and IL-10 were moderately positively correlated with HO-1 concentration (Hb: r = 0.32, p = 0.007; IL-10 r = 0.39, p = 0.0008) whereas HO-1 concentration was weakly negatively correlated with haemopexin (r = −0.23, p = 0.055). Anaemia, while common, was not associated with HO-1 concentration. After adjusting for confounding, HO-1 induction appears to be associated primarily with IL-10 concentration rather than haemolysis. Disease severity at diagnosis was correlated with early plasma IL-10 (r = 0.35, p = 0.003) and HO-1 (r = 0.24, p = 0.048) concentrations. Notably, admission levels of haem, HO-1, and IL-10 were indicators of survival.
Ruthenium-catalyzed selective α-deuteration of aliphatic nitriles using D2O
Authors: Krishnakumar Varadhan , Gunanathan Chidambaram
Published: July 2018

Ruthenium-catalysed selective α-deuteration of aliphatic nitriles using deuterium oxide is reported.
Hydrogen sulfide induced supramolecular self-assembly in living cells
Authors: Wei Simin , Zhou Xi-Rui , Huang Zhentao , Yao Qingxin , Gao Yuan
Published: July 2018

A gasotransmitter mediated reduction instructs supramolecular self-assembly in multiple living cell lines, revealing the variation in intracellular H2S production.
Oxyanion clusters with antielectrostatic hydrogen bonding (AEHB) in tetraalkylammonium hypodiphosphates
Authors: Emami Marzieh , Ślepokura Katarzyna Anna , Trzebiatowska Monika , Noshiranzadeh Nader , Kinzhybalo Vasyl
Published: July 2018

Organic–inorganic salts of hypodiphosphoric acid with tetraalkylammonium cations have been synthesized and characterized by X-ray crystallography and IR spectroscopy.
Fluctuations of random walks in critical random environments
Authors: Mardoukhi Yousof , Jeon Jae-Hyung , Chechkin Aleksei V. , Metzler Ralf
Published: July 2018

Percolation networks have been widely used in the description of porous media but are now found to be relevant to understand the motion of particles in cellular membranes or the nucleus of biological cells. We here study the influence of the cluster size distribution on diffusion measurements in percolation networks.
Computational evidence for the degradation mechanism of haloalkane dehalogenase LinB and mutants of Leu248 to 1-chlorobutane
Authors: Wang Junjie , Tang Xiaowen , Li Yanwei , Zhang Ruiming , Zhu Ledong , Chen Jinfeng , Sun Yanhui , Zhang Qingzhu , Wang Wenxing
Published: July 2018

The catalytic degradation ability of the haloalkane dehalogenase LinB toward 1-chlorobutane (1-CB) was studied using a combined quantum mechanics/molecular mechanics (QM/MM) approach.
Ab initio study of methanol and ethanol adsorption on Brønsted sites in zeolite H-MFI
Authors: Piccini GiovanniMaria , Alessio Maristella , Sauer Joachim
Published: July 2018

Ethanol in zeolite H-MFI: passing from DFT to MP2 and taking anharmonicity into account changes enthalpies of adsorption significantly.
A molecular level study of the phase transition process of hydrogen-bonding UCST polymers
Authors: Sun Wenhui , Wu Peiyi
Published: July 2018

A molecular level study of the UCST-type transition process of PNAGA-based polymers in water and a water/methanol mixture.
Synthesis and catalytic application of alumina@SAPO-11 composite via the in situ assembly of silicoaluminophosphate nanoclusters at an alumina substrate
Authors: Zhang Ping , Liu Haiyan , Zhu Haibo , Oyama S. Ted , Bao Xiaojun
Published: July 2018

An alumina@SAPO-11 composite with a core–shell structure has been synthesized via the in situ assembly and crystallization of silicoaluminophosphate nanoclusters.
Synergistic amplification of catalytic hydrogen generation by a thin-film conducting polymer composite
Authors: Alsultan Mohammed , Choi Jaecheol , Jalili Rouhollah , Wagner Pawel , Swiegers Gerhard F.
Published: July 2018

The presence of PEDOT in a thin-film containing nano-Ni and rGO amplifies catalytic hydrogen generation to exceed that by Pt.
Characteristics of biofilm community structure in a reclaimed water cast iron pipeline
Authors: Liu Dongpo , Rong Chao , Jin Juntao , Liang Sichen , Zhang Jinsong
Published: July 2018

The results indicate that the main biofilm communities in different pipe materials are significantly different from each other. With the passage of time, the richness and diversity of the microbial community in the cast iron pipe biofilm shows a downwards trend.
Sample preparation strategies for petroleum coke digestion and further cerium and lanthanum determination by DSN-ICP-OES
Authors: Souza J. P. , Kellermann K. , Camargo M. S. , Moraes D. P. , Pozebon D. , Pereira J. S. F.
Published: July 2018

Digestion of petroleum coke is proposed for the first time by MW-AD and MIC for La and Ce determination by DSN-ICP-OES.
Superior catalytic performance of a CoOx/Sn–CeO2 hybrid material for catalytic diesel soot oxidation
Authors: Mukherjee Deboshree , Devaiah Damma , Venkataswamy Perala , Vinodkumar T. , Smirniotis Panagiotis G. , Reddy Benjaram M.
Published: July 2018

A Co3O4/Sn–CeO2 hybrid catalyst exhibited superior soot oxidation activity due to the existence of synergism among the multivalent cations and the stepped surface of the hybrid catalyst.
Non-monoterpenoid azepinoindole alkaloids
Authors: Lindsay Ashley C. , Kim Se Hun , Sperry Jonathan
Published: July 2018

This review discusses various biological and chemical aspects of the non-monoterpenoid azepinoindole class of alkaloids, including their isolation, biosynthesis and total synthesis.
Synthesis of dense MoS2 nanosheet layers on hollow carbon spheres and their applications in supercapacitors and the electrochemical hydrogen evolution reaction
Authors: Liu Weiwei , Zhu Menghua , Liu Jinghua , Hu Wenjuan , Li Xin , Liu Jian
Published: July 2018

Dense MoS2 nanosheets are grown on hydrophobic hollow carbon spheres through a solvothermal method for enhanced capacitive storage and electrocatalytic properties.
Fabrication of 3D conductive circuits: print quality evaluation of a direct ink writing process
Authors: Tricot F. , Venet C. , Beneventi D. , Curtil D. , Chaussy D. , Vuong T. P. , Broquin J. E. , Reverdy-Bruas N.
Published: July 2018

Evaluation of the printing quality of a direct writing process developed for the fabrication of 3D conductive circuits.
Facile synthesis and properties of a cation exchange membrane with bifunctional groups prepared by pre-irradiation graft copolymerization
Authors: Zu Jianhua , Tang Fangdong , He Linfeng , Fu Lingxiao
Published: July 2018

Low-cost and anti-degradation ion-exchange membrane named ETFE-g-poly(AA-co-SSS) with bifunctional groups prepared by the pre-irradiation grafting method.
Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products
Authors: Li Ming , Zhang Yuanyuan , Zhao Rujin , Liu Zhenjiang , Hong Xia , Cui Yin , Xue Yonglai , Du Daolin
Published: July 2018

A strategy of immunomagnetic beads coupled with biotin-streptavidin system (BAS-IMB) for detecting AFB1 was suggested and applied in agricultural products.
Photostability and antioxidant activity studies on the inclusion complexes of trans-polydatin with β-cyclodextrin and derivatives
Authors: Li Shujing , Yuan Li , Zhang Bing , Zhou Wei , Wang Xinrui , Bai Dongsheng
Published: July 2018

The inclusion complexes of trans-polydatin and three cyclodextrins (CDs), namely β-cyclodextrin (β-CD), methyl-β-cyclodextrin (Me-β-CD) and (2-hydroxy) propyl-β-cyclodextrin (HP-β-CD) were prepared.
Flexible PANI/SWCNT thermoelectric films with ultrahigh electrical conductivity
Authors: Wu Ruili , Yuan Haocheng , Liu Chan , Lan Jin-Le , Yang Xiaoping , Lin Yuan-Hua
Published: July 2018

Flexible PANI/SWCNT thermoelectric films with ultrahigh electrical conductivity of ~4000 S cm−1. The maximum PF reaches 100 μW m−1 K−2 at 410 K for the 0.6CNT/PANI.
Novel indole based hybrid oxadiazole scaffolds with N-(substituted-phenyl)butanamides: synthesis, lineweaver–burk plot evaluation and binding analysis of potent urease inhibitors
Authors: Nazir Majid , Abbasi Muhammad Athar , Aziz-ur-Rehman Aziz-ur-Rehman , Siddiqui Sabahat Zahra , Raza Hussain , Hassan Mubashir , Ali Shah Syed Adnan , Shahid Muhammad , Seo Sung-Yum
Published: July 2018

In the study presented herein, 4-(1H-indol-3-yl)butanoic acid (1) was sequentially transformed in the first phase into ethyl 4-(1H-indol-3-yl)butanoate (2), 4-(1H-indol-3-yl)butanohydrazide (3) and 5-[3-(1H-indol-3-yl)propyl]-1,3,4-oxadiazole-2-thiol (4) as a nucleophile.
In situ construction of nanonetworks from transformable nanoparticles for anti-angiogenic therapy
Authors: Li Bing-Nan , He Ping-Ping , Yang Pei-Pei , Zhang Jing-Ping , Wang Lei , Wang Hao
Published: July 2018

The defensive networks exhibit transformation-enhanced accumulation and retention (TEAR) effects, and bind and intercept cell-secreted VEGF over particulate and molecular anti-angiogenic agents with high efficiency, leading to anti-angiogenesis.
Biomolecular dynamic covalent polymers for DNA complexation and siRNA delivery
Authors: Bouillon Camille , Bessin Yannick , Poncet Florian , Gary-Bobo Magali , Dumy Pascal , Barboiu Mihail , Bettache Nadir , Ulrich Sébastien
Published: July 2018

Dynamic covalent polymers made from modified amino acids complex nucleic acids and deliver siRNA in living cells.
Hierarchically structured cellulose aerogels with interconnected MXene networks and their enhanced microwave absorption properties
Authors: Jiang Yue , Xie Xi , Chen Yu , Liu Yujing , Yang Rui , Sui Guoxin
Published: July 2018

Hierarchically structured cellulose aerogels with interconnected MXene networks have low densities and enhanced microwave absorption performances.
Linearly polarized photoluminescence from an asymmetric cyclophane showing thermo- and mechanoresponsive luminescence
Authors: Sagara Yoshimitsu , Seki Atsushi , Kim Yuna , Tamaoki Nobuyuki
Published: July 2018

The first cyclophane to exhibit linearly polarized photoluminescence in the liquid-crystalline and crystalline states is described.
Fantastic nuclear envelope herniations and where to find them
Authors: Thaller David J. , Patrick Lusk C.
Published: July 2018

Morphological abnormalities of the bounding membranes of the nucleus have long been associated with human diseases from cancer to premature aging to neurodegeneration. Studies over the past few decades support that there are both cell intrinsic and extrinsic factors (e.g. mechanical force) that can lead to nuclear envelope ‘herniations’, a broad catch-all term that reveals little about the underlying molecular mechanisms that contribute to these morphological defects. While there are many genetic perturbations that could ultimately change nuclear shape, here, we focus on a subset of nuclear envelope herniations that likely arise as a consequence of disrupting physiological nuclear membrane remodeling pathways required to maintain nuclear envelope homeostasis. For example, stalling of the interphase nuclear pore complex (NPC) biogenesis pathway and/or triggering of NPC quality control mechanisms can lead to herniations in budding yeast, which are remarkably similar to those observed in human disease models of early-onset dystonia. By also examining the provenance of nuclear envelope herniations associated with emerging nuclear autophagy and nuclear egress pathways, we will provide a framework to help understand the molecular pathways that contribute to nuclear deformation.
Effects of CNT loading on cellular structures and sound absorption of PU foams
Authors: Basirjafari Sedigheh
Published: July 2018

Efforts have been made to investigate the role of adding carbon nanotubes (CNTs) as the sound energy decaying fillers on the physical and mechanical properties of polyurethane (PU) foam and thus to correlate them with its directly measured sound absorption coefficient. For this aim, the microstructures of PU/CNT nanocomposite foams were investigated and analysed through field emission scanning electron microscopy. Owing to the different microstructures of the samples, large differences were observed in their morphological and physical properties such as density, porosity, pore diameter, closed pore content and thus on their acoustic damping performance.
In‐situ synthesis of silver nanoparticles on porous silicon nanostructure through galvanic displacement reaction and its application in construction of glucose screen printed sensor
Authors: Allahnouri Farzad , Farhadi Khalil , Eskandari Habibollah , Molaei Rahim , Mokhtari Abarghoui Mehdi , Forough Mehrdad
Published: July 2018

In this work, a new nanocomposite as silver nanoparticles (AgNPs)@ porous silicon (PSi) was synthesised through a simple galvanic displacement reaction between silver ions and silicon in the presence of hydrofluoric acid. The as‐prepared nanocomposites were characterised via field emission scanning electron microscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, X‐ray photoelectron spectroscopy, energy dispersive X‐ray spectroscopy and electrochemical methods. Since the nanocomposite showed a greatly enhanced electrocatalytic activity towards the glucose oxidation in alkaline solution, Ag nanoparticles supported on PSi flour were used as a performance modifier in the construction of a new amperometric glucose screen‐printed carbon electrode. The enzymatic free glucose sensor based on AgNPs@PSi exhibited a wide linear dynamic range of 1 × 10−6 –45.7 × 10−3 M, with a detection limit of 6 × 10−7 M at a signal‐to‐noise ratio of 3, and fast response (<3 s). Also, the response of the proposed sensor was selective towards glucose in the presence of common species such as ascorbic acid, dopamine, uric acid and Cl− ions. Finally, the sensor was successfully applied to determine glucose in human serum samples.
Polypyrrole‐modified magnetic nanoparticles for preconcentration of atorvastatin in human serum prior to its determination using high‐performance liquid chromatography
Authors: Ghayb Gholami Shima , Ahmadi Shahin
Published: July 2018

In the current study, the successful application of polypyrrole‐Fe3O4 nanoparticles (NPs) was investigated as a suitable sorbent in the magnetic‐dispersive solid phase extraction mode to the preconcentration and determination of atorvastatin (AT) in human serum by high‐performance liquid chromatography‐ultraviolet detection. Iron oxide NPs were prepared by co‐precipitation method and the polypyrrole compound was used to modify their surface. The structure and NP size of Fe3O4@PPy NPs were characterised by Fourier‐transform infrared technique and scanning electron microscopy. Some factors affecting the extraction efficiency, including the pH value, amount of sorbent, extraction time, elution type and its volume and desorption time were optimised. Under the optimum conditions, magnetic NPs extraction of standard solution of AT showed a linear calibration curve in the range of 0.1–1000 μg l−1 with R 2 = 0.9962. The method was sensitive, with a low limit of detection (0.10 μg l−1) and quantification (0.38 μg l−1). The relative standard deviation of five extractions at the concentration level of 0.1 μg l−1 was 4.2. Good recoveries (92.00–98.10%) with low relative standard deviations (6.0–2.4%) indicated that the matrices do not significantly affect the extraction process. Finally, the proposed method was successfully used to measure AT in human serum samples.
Validity of the RSA-RANDOM Test for Young Soccer Players
Authors: Martin Victor , Sanchez-Sanchez Javier , Ramírez-Campillo Rodrigo , Nakamura Fábio , Gonzalo-Skok Oliver
Published: July 2018

The present study aimed to examine the reliability, usefulness, responsiveness, age-related differences and construct validity of a novel decision-making RSA test (RSA-RANDOM test) in young soccer players. Twenty-five young male soccer players performed the RSA-RANDOM test on 2 occasions separated by 5–7 days to assess test-retest reliability and determine a priori usefulness. Furthermore, the same players executed the RSA-RANDOM test 4 times throughout the season to analyse responsiveness. Forty-five players (U–13 to U–17) were evaluated in such test to examine age-related differences. Finally, 9 players were used to determine the construct validity of the test. Reliability scores showed a high intraclass correlation coefficient (ICC=0.88 to 0.90) and low coefficient of variation (CV=1.0–1.2%). The responsiveness of the RSA-RANDOM test was good, as the typical short- (1.2–1.9%), mid- (1.4–2.4%) and long-term (2.3–3.2%) changes in RSA-RANDOM performance were higher than the CV. Age-related differences analysis showed better RSA-RANDOM performance as age increased in young soccer players. Low (r=−0.50) to moderate (r=−0.75) relationships were found between the RSA-RANDOM test variables (RSA best and mean times) with high-intensity and total distance covered, respectively. A novel decision-making RSA test (RSA-RANDOM) has shown to be reliable and valid in young soccer players.
Vitamina D tem influência nos implantes dentários?
Authors: Bubola Julia , Ferreira Rafael , Sant'Ana Adriana , Rezende Maria , Greghi Sebastião , Damante Carla , Zangrando Mariana
Published: July 2018

A vitamina D (calciferol) possui papel importante no metabolismo ósseo (turnover), mantendo os níveis de cálcio e fósforo no sangue. A principal fonte de produção é pela pele estimulada pelos raios solares, porém, as características da vida moderna como o receio de danos dermatológicos e uso de agentes protetores têm dificultado/diminuído a exposição ao sol. Desta forma, há o comprometimento da síntese adequada dessa vitamina, o que pode influenciar negativamente no sucesso clínico de implantes dentários. O objetivo dessa revisão de literatura é abordar a importância da vitamina D e o efeito de sua deficiência sobre a saúde sistêmica e a repercussão na cavidade bucal com implantes dentários. Para isso, foi realizado uma busca nas bases de dados Scielo, Pubmed e Scopus com os descritores: “deficiency and vitamin D and dental implants”. Foram selecionados 13 artigos para revisão e análise dos dados. A carência de vitamina D gera inúmeras alterações, desde hipocalcemia leve, perda de osso trabecular, como a quadros graves com comprometimento ósseo, muscular, imunológico e metabólico. No caso de suspeita de deficiência de vitamina D no paciente que irá receber ou possui implante dentário, deve-se encaminhar o paciente a um médico e nutricionista, que analisará a existência real da hipovitaminose e indicará uma adequada abordagem terapêutica. Os estudos in vitro e em animais demonstram os efeitos da deficiência ou efeito da suplementação da vitamina D no metabolismo ósseo ao redor de implantes dentários. Já os estudos em humanos apresentam grande heterogenidade, não apresentando uma ligação entre baixos níveis séricos de vitamina D com o risco aumentado na perda precoce de implantes. Portanto, são necessárias mais pesquisas, incluindo investigações clínicas longitudinais e revisões sistemáticas, que possam elucidar melhor os mecanismos de ação da vitamina D nos processos de metabolismo ósseo e de osseointegração, auxiliando cirugiões-dentistas, médicos e nutricionistas quando em contato com os pacientes que utilizam ou irão utilizar implantes dentários.
Avaliação dos níveis de ferritina e vitamina B12 em pacientes submetidos à cirurgia bariátrica suplementados com polivitamínico
Authors: Reis Maria , Almeida Júlia , Amorim Maria , Amaral Daniela , Gregório Eric
Published: July 2018

As carências nutricionais de ferro e vitamina B12 são as principais alterações que colocam em risco o sucesso da cirurgia bariátrica. O objetivo desse estudo foi avaliar os níveis da ferritina e da vitamina B12 em pacientes com obesidade grau III do Sistema Único de Saúde (SUS), submetidos à derivação gastrojejunal em Y de Roux (BPGYR), suplementados gratuitamente com o produto Materna®. Esse polivitamínico foi distribuído aos 40 pacientes gratuitamente pelo período de 24 meses e 23 pacientes, o grupo controle, não receberam o medicamento. A comparação da ferritina e vitamina B12 em pacientes submetidos ao BPGYR, com suplementação gratuita foram superiores a aqueles observados para o grupo controle. Conclui-se que o fornecimento gratuito do Materna pelo SUS aos pacientes submetidos ao BPGYR é uma medida recomendada para o controle adequado e a aderência do paciente ao tratamento.
Knowledge, Attitudes and Practices of Primary School Teachers on Nutrition and Food
Authors: Falkenbach Danielle , D'avila Helen , Mello Elza
Published: July 2018

Introdution The teacher's formation is a paramount condition for the education in health.

Objective To develop a questionnaire and assess knowledge, attitudes and practices of early years school teachers.

Methodology a questionnaire about nutrition and feeding was applied. The subjects were teachers from pre-school to 5th grade of Elementary School.

Results There were 288 teachers in the survey. They were all in favor of the promotion of a healthy feeding in school. The source of information mostly used was the textbook. 76% thought they had good knowledge about a healthy diet. The average number of positive/correct answers was 21 (48%).

Conclusions The knowledge about nutrition and feeding were insufficient and the general performance in the questionnaire was low. Actions that aim at qualifying the teachers are urgent, so that they can develop the necessary tools to promote a healthy feeding in schools.
Unión cráneo-cervical – anatomía normal y correlación con imágenes
Authors: Sgarbi Nicolás , Telis Osmar
Published: July 2018

La solicitud de estudios de imagen en pacientes con trauma cervical es muy frecuente en la práctica diaria. Esa patología es causa relativamente frecuente de discapacidad en pacientes jóvenes junto con el trauma encéfalo craneano. En un porcentaje no despreciable de los casos, las lesiones traumáticas comprometen la unión cráneo-cervical y en esos pacientes, la morbi-mortalidad es más significativa. La transición entre el cráneo y el raquis se basa en un conjunto de estructuras óseas relacionadas por articulaciones muy móviles y estabilizadas por un grupo de ligamentos y músculos que le brindan al mismo tiempo gran solidez. Para una correcta interpretación de los estudios de imagen de uso corriente en la clínica, es fundamental un sólido conocimiento anatómico de la unión cráneo-cervical y sus componentes. Es el objetivo de esta revisión, sistematizar la anatomía de la unión cráneo-cervical con especial énfasis en sus ligamentos, analizar la fisiología de sus movimientos y el concepto de estabilidad para luego realizar una correlación con tomografía computada multi-detector y resonancia magnética.
Prevention of Postoperative Deep Vein Thrombosis by a Combination of Subcutaneous Heparin with Subcutaneous Dihydroergotamine or Oral Sulphinpyrazone
Authors: Veth Gerda , Meuwissen Otger J A Th , van Houwelingen Hans C , Sixma Jan J
Published: July 2018

In a randomized clinical trial the effect of subcutaneous heparin alone or in combination with dihydroergotamine or sulphinpyrazone in preventing postoperative deep vein thrombosis (DVT) was studied. Sodium heparin (5000 IU) was administered subcutaneously twice daily; dihydroergotamine (1/2 mg) was also administered subcutaneously twice daily, and sulphinpyrazone (400 mg) was administered orally or intravenously twice daily. Administration occurred for at least 7 days. The diagnosis DVT was made with the radiofibrinogen uptake test. 358 patients undergoing major elective abdominal surgery were allocated to three treatment groups: heparin alone (Hep), heparin + dihydroergotamine (DHE-Hep) and heparin + sulphinpyrazone (Sulph-Hep). The frequency of DVT was 14/114 in Hep, 10/115 in DHE-Hep and 20/114 in Sulph-Hep. These differences were not significant. After application of the “logistic regression” procedure of Cox (1) it turned out that the major risk factors for developing DVT were age, sex, weight, type of operation and presence of diabetes mellitus. Also a significant treatment influence was observed (p = 0.001). This treatment effect was most probably due to improvement in the DHE-Hep group.

The results in the Sulph-Hep group were not significantly different from those in the Hep group. A risk index was formulated on the basis of the above mentioned risk factors by which the chance of occurrence of DVT during heparin prophylaxis in an individual patient could be predicted. Patients that should receive additional prophylactic treatment can be defined by using this risk index.
Studies on Platelets of Patients with Inherited Platelet Disorders Suggest that Collagen-Induced Fibrinogen Binding to Membrane Receptors Requires Secreted ADP but Not Released α-Granule Proteins
Authors: Legrand Chantal , Nurden Alan T
Published: July 2018

Collagen induces a saturable 125I-fibrinogen binding to normal human platelets. A role for secreted ADP in this process is supported by studies on 2 patients with the Chédiak-Higashi syndrome. Both collagen-induced nucleotide release and 125I-fibrinogen binding were strongly reduced while ADP-induced fibrinogen binding was normal. Platelets from 2 patients with the gray platelet syndrome bound normal amounts of 125I-fibrinogen in the presence of ADP or collagen despite the severe reduction of secretable α-granule proteins. Binding did not occur to collagen-stimulated type I thrombasthenic platelets which lacked GPIIb-IIIa complexes but was detected in amounts which correlated with the residual concentrations of GPIIb-IIIa in the platelets of a patient with type II disease. Our results allow us to propose that collagen-induced fibrinogen binding to normal platelets requires the presence of GPIIb-IIIa complexes and secreted ADP but proceeds independently of α-granule release.
Protein C Antigen Levels in Major Abdominal Surgery: Relationships to Deep Vein Thrombosis, Malignancy and Treatment with Stanozolol
Authors: Blamey S L , Lowe G D O , Bertina R M , Kluft C , Sue-Ling H M , Davies J A , Forbes C D
Published: July 2018

Activated protein C is a potent inhibitor of coagulation, and familial protein C deficiency has been associated with recurrent venous thrombosis. We have investigated protein C antigen levels in patients undergoing major elective abdominal surgery, to determine their relationships to postoperative deep vein thrombosis (DVT), malignancy, and preoperative treatment with intramuscular or oral stanozolol. Preoperative and postoperative protein C levels were not significantly different in patients with and without DVT (detected by 125I-fibrinogen leg scans), nor in patients with and without malignancy. In a placebo group (n = 26), a significant fall in protein C was maximal on the first postoperative day and persisted for 7 days. In a group given intramuscular stanozolol, 50 mg on the preoperative day (n = 23) stanozolol shortened the duration of the postoperative fall in protein C, but did not prevent DVT. In a group given oral stanozolol, 10 mg/day for 2 weeks before and 1 week after operation (n = 11), stanozolol significantly increased protein C levels prior to surgery, hence maintaining protein C at pretreatment levels after surgery. The effect of this regimen on the incidence of DVT is under study.
Hepatic Clearance of Tissue-Type Plasminogen Activator in Rats
Authors: Emeis J J , van den Hoogen C M , Jense D
Published: July 2018

The clearance of tissue-type plasminogen activator (t-PA) was studied, in rats, by use of a functional assay for t-PA. Half-lives in the circulation were about one minute both for human (melanoma cell-derived) t-PA and for the rat’s own t-PA. The clearance of t-PA required an intact liver blood flow. In isolated liver perfusion experiments the hepatic extraction of t-PA did not require any plasma proteins, including fast-acting t-PA inhibitor. Competition experiments, using monosaccharides, suggested that known hepatic glycoprotein receptors were not involved in hepatic t-PA extraction.
The Heparin-Mobilisable Pool of Platelet Factor 4: A Comparison of Intravenous and Subcutaneous Heparin and Kabi Heparin Fragment 2165
Authors: O’Brien J R , Etherington M D , Pashley Michelle A
Published: July 2018

Some clinical advantages are claimed for low molecular weight heparin so the mobilisation of platelet factor 4 (PF 4) from the endothelial pool by the heparins may be relevant. Unfractionated (UF) heparin has been compared with Kabi heparin fragment 2165. A single intravenous (i. v.) injection of 60 iu/kg heparin was compared with 5000 anti-Xa units of Kabi-2165. Less PF 4 was mobilised by Kabi-2165 and some apparently remained in the pool and was released when the pool was subsequently challenged by giving i.v. heparin. Subcutaneous (s. c.) injections of 5000 iu heparin twice daily were compared with 5000 anti-Xa units of Kabi-2165 once daily, each given for a week. The plasma PF 4 was never raised yet when finally challenged with i.v. heparin the pool was “empty” or refractory after the s.c. heparin but some PF 4 remained after the s.c. Kabi-2165. The two glycosaminogly-cans (GAGs) had widely differing half-lives but the t/2 of the PF 4 mobilised by the two GAGs was similar even though the PF 4 is apparently bound to the GAG.
Fibrinopeptide A in Urine from Patients with Venous Thromboembolism, Disseminated Intravascular Coagulation and Rheumatoid Arthritis - Evidence for Dephosphorylation and Carboxyterminal Degradation of the Peptide by the Kidney
Authors: Leeksma O C , Meijer-Huizinga F , Dalen E A Stoepman-van , Aken W G van , Mourik J A van
Published: July 2018

Urinary fibrinopeptide A immunoreactivity was determined by radioimmunoassay using two anti-fibrinopeptide A sera with a different specificity in patients with venous thromboembolism, disseminated intravascular coagulation and rheumatoid arthritis. Elevated levels were frequently observed with both sera, and intravenous administration of heparin in patients with a thromboembolic disorder resulted in a decline of urinary fibrinopeptide A (FPA) concentrations to normal or nearly normal values. For both sera significant correlations with plasma levels were found although one of the sera reacted significantly better with the material in urine samples from these patients than the other (p <0.0001, n = 73). Analysis of urinary fibrinopeptide A immunoreactivity by high performance liquid chromatography (HPLC) provided evidence that A peptide material present in this body fluid was heterogeneous. In view of the characteristics of the antisera used in this study, data suggest that urinary FPA immunoreactivity consists to a large extent of carboxyterminally degraded FPA. Excretion of circulating FPA immunoreactive material through the kidneys apparently involves dephosphorylation and carboxyterminal breakdown of the A peptide. Since both synthetic and native phosphorylated or unphosphorylated fibrinopeptide A appeared to be stable in urine in vitro, an active role of the kidney in degrading the A peptide is likely.
Soluble Crosslinked Fibrin(ogen) Polymers
Authors: Selmayr Eberhard , Müller-Berghaus Gert
Published: July 2018

The present study is concerned with the formation of fibrin-fibrinogen associations in the presence of FXIIIa while fibrinolysis was inhibited by aprotinin and EACA. SDS agarose-poly acryl-amide gel electrophoresis on 2.5% gels under non-reducing conditions and ultracentrifugation of the associations on urea-sucrose density gradients showed the formation of soluble crosslinked high molecular weight (HMW) fibrin-fibrinogen polymers with an estimated molecular size up to 10 times that of fibrinogen. After incubation of a mixture of 131I-fibrinogen (4 mg/ml) and 125I-desAA-fibrin (0.2 mg/ml) with FXIIIa (2 U/ml) for 1 h at 37ΰ C, about 5% of the fibrinogen and 80% of the fibrin was incorporated into the generated soluble HMW polymers. The detection of soluble crosslinked fibrin-fibrinogen polymers could be a useful diagnostic criterion for imminent DIC.
Uptake and Inactivation of Thrombin on Rabbit Aortic Endothelium Studied with Two Different Substrates
Authors: Swedenborg Jesper , Dryjski Maciej , Frebelius Siw , Olsson Per
Published: July 2018

The endothelium is an important compartment for uptake and inhibition of thrombin. The amount of enzymatically active bound thrombin can be detected with both small synthetic substrates and with aid of fibrinogen as substrate. The present study was designed to investigate the relation between endothelially bound thrombin with amidolytic activity towards a synthetic substrate (S-2238) and thrombin capable of converting fibrinogen by measuring generation of fibrinopeptide A (FPA). The luminal surfaces of rabbit aortae (2 cm2) were exposed in vitro to thrombin (0.625-5.0 NIH units/ml). Thrombin disappeared from the solution and a certain fraction was recovered on the surface. There was a linear relationship between the amount of thrombin on the surface and the concentration of thrombin in the incubation mixture. Approximately one third of the thrombin measured with S-2238 was also able to cleave fibrinogen. After incubation with defibrinogenated plasma almost total inhibition of fibrinogen splitting activity occurred within 30 sec. The inhibition of the amidolytic activity was less complete. When endothelially bound thrombin was exposed to plasma much less FPA was generated than in a fibrinogen solution. A minor fraction of endothelially bound thrombin was inhibited also upon incubation with Tyrode without recovery of any enzymatic activity in the solution. The results indicate that a fraction of thrombin bound to the endothelium has retained enzymatic activity and that a fraction of the enzymatically active thrombin is capable of converting fibrinogen. Inhibition of thrombin enzymatic activity occurs rapidly upon exposure to plasma. The endothelium itself has a minor inhibitory effect also in the absence of plasma. Thrombin with retained fibrinogen splitting activity may contribute to thrombotic events particularly if plasma inhibitors are insufficient. Thrombin with activity towards fibrinogen could be bound to GAG:s whereas thrombin active towards S-2238 could be bound to GAGrs TM or possibly other receptors.
The Effect of Heparin vs. Citrate on the Interaction of Platelets with Vascular Graft Materials
Authors: Kottke-Marchant Kandice , Anderson James M , Rabinovitch Albert , Huskey Richard A , Herzig Roger
Published: July 2018

Heparin is known to affect platelet function in vitro, but little is known about the effect of heparin on the interaction of platelets with polymer surfaces in general, and vascular graft materials in particular. For this reason, the effect of heparin vs. citrate anticoagulation on the interaction of platelets with the vascular graft materials expanded polytetrafluoroethylene (ePTFE), Dacron Bionit (DB) and preclotted Dacron Bionit (DB/PC) was studied in a recirculating, in vitro perfusion system. Platelet activation, as shown by a decrease in platelet count, an increase in platelet release and a decrease in platelet aggregation, was observed for all vascular graft materials tested using heparin and was greater for Dacron and preclotted Dacron than for ePTFE. Significant differences between heparin and citrate anticoagulation were seen for platelet release, platelet aggregation and the relative ranking of material platelet-reactivity. However, the trends and time course of platelet activation were similar with both heparin and citrate for the materials tested.
Anticoagulant Properties of Extracellular Slime Substance Produced by Staphylococcus Epidermidis
Authors: Bykowska K , Ludwicka A , wegrzynowicz Z , Lopaciuk S , Kopeć M
Published: July 2018

Slime produced by S. epidermidis strain KH 11 was extracted with phenol-saline. The saline phase was fractionated on a DEAE-Sepharose CL-6B column. The crude slime solution and its phenol-saline fraction were found to possess anticoagulant properties. They inhibited the coagulation of human plasma by thrombin, prolonged the activated partial thromboplastin time, but did not change the rate of plasma coagulation by reptilase. The anticoagulant effect of slime could be neutralized by rather high concentrations of protamine sulphate. In the presence of plasma, the staphylococcal slime also inhibited in a concentration dependent fashion the amidolytic activity of thrombin and factor Xa against synthetic chromogenic substrates. Both antithrombin III (AT III) and other plasma component(s), presumably heparin cofactor II, were required for the full expression of the slime effect. The anticoagulant action of slime was markedly less AT III dependent than that of heparin. The activity was resistant to heating (100° C, 30 min). Slime and its fractions were stronger inhibitors of thrombin than of factor Xa. Fraction IV separated by DEAE-Sepharose chromatography and particularly rich in galactose and glucuronic acid had the highest inhibitory potency. It is conceivable that slime component(s) similar to glycosaminogly-cans from other sources can carry the anticoagulant activity.
The Use of a Functional and Immunologic Assay for Plasma Protein C in the Study of the Heterogeneity of Congenital Protein C Deficiency
Authors: Bertina R M , Broekmans A W , Es C Krommenhoek-van , Wijngaarden A van
Published: July 2018

Protein C is a vitamin K dependent protein involved in blood coagulation. A congenital deficiency in protein C antigen - which inherits as an autosomal dominant disorder - has been reported to be associated with a high risk for thrombo-embolic disease at relatively young age. In the present paper we report on the development of a functional assay for plasma protein C. In this assay protein C is adsorbed to Al(OH)3, eluted and activated by thrombin, after which the concentration of the activated protein C is measured with a peptide substrate (S2366). Normal values for protein C activity and protein C antigen were determined in healthy volunteers and patients on stable oral anticoagulant treatment. Protein C activity and antigen levels were compared in 28 patients from 9 different pedigrees with both congenital protein C deficiency and thrombotic disease. Two types of protein C deficiency could be recognized: in type I the deficiency is due to the absence or reduced presence of protein C molecules, while in type II the deficiency is caused by the presence of an abnormal protein C molecule with strongly reduced functional activity.