bluuebunny/crossref_metadata_2025 huggingface dataset:
Inducible Nitric Oxide Synthase (iNOS) Expression in Cirrhotic Patients with Hepatopulmonary Syndrome
Authors: Sun Li Fang , Zhang Bing Quan , Zhang Na , Wang Yan Ying , Yu Jing
Published: January 2011

The aim of this study was to define that the expression of inducible nitric oxide synthase (iNOS)in cell blocks from ascites was a certain value to evaluate hepatopulmonary syndrome(HPS). iNOS can stimulate the liver and lung to produce excess NO. Excessive production of NO can cause pulmonary vasodilatation and impaire arterial oxygenation. HPS is characterized by shortness of breath and hypoxemia caused by impaired arterial oxygenation due to pulmonary microvascular dilatation in patients with liver disease or PTH. Therefore.the positive rates of iNOS was a certain value to evaluate the conditions of HPS.
The Effect of Physical Properties on the Interfacial Adhesion of Fiber Reinforced Resin Composites
Authors: Xu Li Wei , Huang Y.D , Liu Li
Published: January 2011

The effect of physical properties on the interfacial adhesion properties of fiber reinforced nylon66 composites have been investigated in this paper. The microstructure of fibers was observed using scanning electron microscopy (SEM), atomic force microscopy (AFM) and energy dispersive X-ray (EDX). The results revealed that smooth sizing with some tiny and uniform protuberances can enlarge the surface area of the glass fiber, and increased the wettability of resin to glass fiber, so as to increase the interfacial adhesion properties of the glass fiber/resin composites. In addition, the properties of the intheface between glass fiber and composites have been studied by interfacial shear strength (IFSS) and dynamic wetting method.
Study on Old Newspaper Recycling Fiber-Reinforced PLA Composites
Authors: Li Xin Gong , Li Hui , Cheng Fang , Tong Zhuo
Published: January 2011

In the research, old newspaper recycling fiber-reinforced polylactic acid composites were produced by using injection molding technology. The influences of MAPP coupling agent and newspaper alkali deinking treatment on the performance of composite materials were studied. The results showed: MAPP can effectively improve the compatibility of old newspaper recycling fibers and PLA and increase the mechanical properties of composites; the alkali deinking treatment can effective improve the adhesion of newspaper recycling fibers and PLA and increase the mechanical properties of composites.
Application of TM Images on Estimation of Organic Carbon in Surface Soil in Burned Areas
Authors: Sun Long , Zhang Ya Nan , Hu Hai Qing
Published: January 2011

Based on remote sensing and field measured data, this paper discussed the effectiveness of selecting optimal variables with a cross-variable by VIP≥1 and used partial least squares regression (PLSR) to build a model for estimating surface soil organic carbon of burned area. The results showed that: variables such as K-T1, K-T2, TM2, TM4, and TM5/TM4 have larger contribution to the model, their VIP values were 1.5116, 1.1915, 1.3545, 1.2242 and 1.4275, respectively. The intensity index of Tasseled Cap transformation has a higher value than the original bands of TM2 and TM4, and the contribution of bands ratio is higher than the single band. Further, the PLS model was applied to estimating the special distribution of soil organic carbon (SOC) content (unit in g.kg-1) in entire study area. We conclude that different fire intensity affected on soil organic carbon variously, and followed an order as high intensity>moderate intensity>light intensity. The average density of surface soil organic carbon was 12.53 kg per m2, soil organic carbon storage was 202.46×106kg, the fire-released soil organic carbon was 29.8×106kg in study area.
Preparation, Characterization of Nonmetal Doped TiO2 Nanoparticles with their Excellent Photocatalystic Properties
Authors: Wang Ying Wei , Li Yu Fei , Yang Pei Han
Published: January 2011

Nonmetal (S, P) doped titania nanoparticles were synthesized by a one step hydrothermal method. These samples were calcined with different temperature, the sample exist in anatase phase has much higher photocatalytic activity for methylene blue (MB) degradation. The visible response and the higher UV activity of the different nonmetal doped TiO2make it possible to utilize solar energy efﬁciently to execute photocatalysis processes. The resulting materials were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), etc. It can conclude the nonmetal doping TiO2proves to be more suitable to improve the photocatalytic performance.
Effect of FA/O Chelating Agent on Copper Ion Removing on Silicon Surface
Authors: Tan Bai Mei , Niu Xin Huan , He Yan Gang , Gao Bao Hong , Liu Yu Ling
Published: January 2011

Along with the feature size reducing and the increase of integration level rapidly in ULSI，the request for metal impurities contamination on silicon substrate surface appears specially rigorous. In this paper the chelating agent was added in cleaning solution in order to removing copper ion. FA/O, a new kind of chelating agent was studied in RCA cleaning solutions, which has 13 chelating rings and is free of sodium, stable and easily soluble. The XPS and GFAAS measured results indicate that FA/O is more efficient than NH4OH as a ligand. Cu contaminations on silicon wafer can be removed remarkably when adding a little FA/O to the cleaning solution or polishing slurry. When the chelating agent concentration of cleaning solution is 0.1% the removal rate of Cu atom reaches 83 percent. The FA/O chelating agent substituting NH4OH in SC-1 may simplify cleaning steps, and one cleaning step can remove Cu pollution on silicon wafer surface and meet the requirements of microelectronics technology.
Ultrasonic Welding Performance Analysis of Wood-Flour/PP Composites
Authors: Zhao Hui , Zhou Yi Long , Zhu Hong Yuan , Wang Qing Wen
Published: January 2011

In order to analyze the possibility of ultrasonic welding of wood-plastic composites, wood-flour(WF)/ polypropylene(PP) composite was ultrasonic welded, which contained 60%WF, 36%PP and 4% maleic anhydride grafted PP. The ultrasonic welding performance was evaluated by tensile tests and scanning electronic microscopy(SEM). The triangle energy direct stick was used in the ultrasonic welding. The results showed that WF/PP composite was successfully ultrasonic welded directly, while in the presence of energy direct stick, the strength of the welded joint was even better than the composite itself. It was concluded that the ultrasonic welding was suitable to WF/PP composites.
Prediction and Positive Analysis of Security Housing Requirement for Stepped leveling Urban Floating Population
Authors: Wang Wen Xia , Tian Jin Xin , Li Xiao Ming
Published: January 2011

With the speeding urbanization process in China, the amount of floating population increases at an unprecedented speed. How to solve the housing security issue now becomes a key indicator for social stability. In this paper, a new low-income housing requirement prediction method is proposed based on stepped leveling of floating population and related evaluation index predictions. Using Logistic retardant population growth model and Todaro method, floating population in the next 5 years is predicted. Then the population is divided into stepped levels to predict housing requirement. Based on the prediction of commercial house price, the method of predicting low-income house demand for different levels in urban areas is given. To demonstrate the proposed model and method, Shanghai Statistical data over the years are used and analyzed.
Study of Esculetin-Induced Apoptosis in Human Gastric Carcinoma SGC-7901 Cells
Authors: Jia Shao Hua , Ji Yu Bin
Published: January 2011

In order to investigate the effect and its mechanism of esculetin on human gastric carcinoma SGC-7901 cells, the effect of esculetin on human gastric carcinoma SGC-7901 cells were investigated by MTT method in vitro studies. Then the morphological feature changes of apoptosis cells with esculetin of different concentration by transmission electron microscope were observed and the levels of Cty-c in SGC-7901 cells line using FCM method were analyzed. The results revealed that the Esculetin significantly inhibited the growth of human gastric carcinoma SGC-7901 cells and greatly decreased its growth rate with the esculetin concentration increased, and then the morphological feature changes of apoptosis cells had been observed. After 24h, esculetin could make that the concentration of Cty-c in endochylema and Caspase-3 were all increased. Thus the study showed that esculetin remarkably inhibited the growth of human gastric carcinoma SGC-7901 cells and induce its apoptosis.
The Development of Compatible Tree Biomass Models for Main Species in North-Eastern China
Authors: Li Feng Ri , Liu Fu Xiang , Jia Wei Wei
Published: January 2011

In accordance with weakness of traditional biomass models in which the sum of the above- and below-ground tree components was not equal to the whole tree, compatible biomass equations were developed for the above- and below-ground tree components of 11 kinds of tree species in Heilongjiang Province. The data used to develop biomass models are from 299 trees that were collected from 69 sample plots, and represented a wide range of stand and site conditions in Heilongjiang Province. Based on the total biomass model as restrictions, the compatible tree biomass equations for each component (stems, branches, foliages, and roots) were separately established by considering diameter at breast height (D) as independent variables in the form of simultaneous equations with measure error of independent variables. The evaluation and validation procedures for tree biomass models were performed by using the following statistical criterions: the coefficient of determination (R2), sum square of error (SEE), Mean Bias (MB), and Mean Absolute Bias (MAB). The results showed that although parameter estimation accuracy of compatible biomass equations was not better than traditional biomass models, the compatible biomass equations could effectively overcome the weakness of un-compatible of traditional biomass models.
Development of Pinus koraiensis SSR Primers Based on EST-SSR Information Technology
Authors: Sui Xin , Feng Fu Juan , Zhao Dan , Chen Min Min , Han Shi Jie
Published: January 2011

A total 408 SSRs were distributed in 18,181 ESTs sequences in Pinaceae in NCBI (National Center for Biotechnology Information) searched by SSRIT software, accounting for 2.24% of the whole EST sequences. We designed 132 pairs of EST-SSR primers by primer3. Of the designed 132 pairs, 29 pairs were able to produce an amplification product in the 10 Pinus koraiensis DNA samples, but only five primers in the 29 pairs exhibited polymorphism. Dinucleotide repeats were the most common repeat class. The repeated primitives of dinucleotide were 10, accounting for 52.73% of the whole repeated primitives; the repeat numbers were 87. The second most common repeat class was trinucleotide. The repeated primitives of trinucleotide were 27, accounting for 42.27% of the whole repeated primitives, and repeat numbers were 78.
The Operation Time Influence on the Sauce Wastewater Treatment
Authors: Liu Zhi Xiao , Zuo Jin Long
Published: January 2011

With rapid development of food industry, the production of soybean sauce is increasing in recent years. The sauce wastewater is doing greater and greater harm to the water environment. In order to tackle this problem, the operation time on the sauce wastewater treatment were investigated. The results showed that the process has a better effect for ammonia nitrogen, the orthophosphate and COD removal. The effluent ammonia nitrogen was less than 5mg/L and the ammonia nitrogen removal efficiency could reach about 90% with the aeration time 2 h-3.5 h. The orthophosphate increased during the anoxic stage while decreased during the aerobic stage. At the end of the aerobic stage, the orthophosphate concentration and the COD could reach about 1mg/L and 21 mg/L respectively when aeration time was 2 h-3.5 h. The better operation time (the aeration time) was at 2 h-3.5 h and the system could get a good water quality for sauce wastewater treatment.
Sludge Reduction by Electrolysis in Activated Sludge Process
Authors: Cheng Li Hua , Bi Xue Jun , Liu Chang Qing
Published: January 2011

Sludge reduction by electrolysis and its subsequent influence on removal rate of COD, NH3-N, PO43- and on the sludge activity in the aeration tank were investigated. In this research, two parallel sequencing batch reactors were used with one set was operated in convention modality and the sludge of the other set was electrolyzed and then fed back to the reactor. The results showed that: sludge electrolyze could reduce the sludge production around 21% and had slight effect on substrate removal efficiency. After the sludge being electrolyzed, removal rate of COD and NH3-N decreased by 1.1% and 6.9%, respectively. PO43- removal rate increased by 6.1%. The number of protozoa, metazoan and protonema decreased and the structure of sludge floc became more compact. Moreover, the activity of microorganism and carbon source metabolism of sludge changed slightly, but the microbial community structure and metabolic function of microorganism had little change.
Bioavailability of Phosphorus Fractions for Incepting of Chlorella pyrenoidosa in the Lower Reaches of the Hanjiang River in China
Authors: Tian Jian Ru , Chen Guo Li , Kang Guang Feng , Zheng Xiu Ping
Published: January 2011

Using total phosphorus (TP) concentrations to estimate eutrophication risk is problematic for management purposes, as only some forms of P are biologically available for phytoplankton growth. This study estimated the bioavailability of P fractions of water and the floodplain sediment in the lower reaches of the Hanjiang River in China using multiple regressions. And the effects of concentrations of P fractions on algal growth were also studied. The results showed that the total soluble P (TSP) and the particulate P (PP) can well predict the growth of Chlorella pyrenoidosa in water samples. And the exchangeable P (exch-P) and the P bound to Al, Fe, and Mn oxides and hydroxides (Fe/Al–P) were adapted to predicting the growth of Chlorella pyrenoidosa in the floodplain sediment samples.
Comparative Analysis and Improvement of C/N on Hydrogen Production with the Bacterium Strain B49
Authors: Li Yong Feng , Duan Yi Tong , Liu Fang Jing , Wang Lu , Wang Yi Xuan
Published: January 2011

Anaerobic process of biohydrogen production was developed recently. The representative strain of hydrogen production bacteria, namely fermentative hydrogen producing bacterial strain B49，was isolated from the activated sludge which was cultured artificially by our lab. In this study, there were some different C/N ratios employed. Experiment results indicated that the concentration of carbon and nitrogen had a great effect upon hydrogen production of B49, when C/N=2.5, glucose got the highest degraded efficiency, and at a C/N ratio of 3.3, the bio-gas and hydrogen production peaked with 159mL and 37.5mL, respectively, which considered that the optimum C/N ratio on the growth and hydrogen production was 3:1. Furthermore, the mainly terminal liquid products of B49 were ethanol and acetic acid. With the increase of the total organic nitrogen, final pH value decreased gradually.
Effects of Lymantria dispar Feeding and Wounding on Phenyalanine Ammonia-Lyase in Populus simonii × P. nigra
Authors: Cao Chuan Wang , Ma Ling , Yan Shan Chun , Wang Zhi Ying
Published: January 2011

In plants, phenyalanine ammonia-lyase (PAL) has been related to defense mechanisms against insect feeding and wounding, and its role was investigated in poplar (Populus simonii × P. nigra) regarding induction by third-instar Lymantria dispar larvae feeding and punching mechanical wounding. The activity and mRNA expression level of PAL was evaluated during time courses in relation to L. dispar feeding and mechanical wounding. The constitutive levels of PAL activity in healthy poplar showed no significant differences during 72 h. L. dispar feeding and mechanical wounding caused different responses of protein and transcript levels of PAL. PAL was obviously induced by L. dispar feeding during a 24-72 h period. For mechanical wounding, activity and mRNA expression level of PAL were not significant induced but were mostly inhibited. The results suggested PAL gene may be significantly related to poplar defense against L. dispar attack.
The Preparation of Hydrolyzed Carp Protein
Authors: Li Ci Li , Han Bing
Published: January 2011

The carp was used to prepare fish protein hydrolysate in the article. The results demonstrated that the optimum conditions for preparation of hydrolyzed fish protein with Neutrase by orthogonal test are as follows: the temperature is 50°C, the enzyme content is 2.0%, the pH value is 7.5, the hydrolyzed time is 5 hours, the weight ratio of meat to water is 1:6. Under the conditions, the recovery of hydrolyzed protein was 94.28%.
Removal of Rhodamine B from Wastewater by Rectorite and Sepiolite-Kinetic Study and Equilibrium Isotherm Analyses
Authors: Li Jun , Hu Ming Zhen
Published: January 2011

Adsorption removal of a cationic dye, rhodamine B (RhB) from water onto rectorite and sepiolite was investigated. The rectorite and sepiolite were characterized by Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). Attempts were made to fit the isothermal data using Langmuir and Freundlich equations. The experimental results have demonstrated that the equilibrium data are fitted well by a Freundlich isotherm equation. Pseudo-first-order and pseudo-second-order models were considered to evaluate the rate parameters. The experimental data were well described by the pseudo-second-order kinetic model. The results indicate that the rectorite exhibited higher adsorption capacity for the removal of RhB than sepiolite and could be employed as a low-cost alternative in wastewater treatment for the removal of cationic dyes.
Study on the Impact on Environment in Ductile Iron Production
Authors: Li Chang Long , Dong Liang , Sun Xiao Min
Published: January 2011

Using the invention patent technology (patent number ZL2005 1 0131175.7), the impact on environment in ductile iron production was studied. By using the environmentally friendly and adjustable spheroidizing equipment, the spheroidization can proceed in a completely closed state. The smoke with the spheroidization is eliminated just as the production of inoculated cast iron. The pressure in the ladle can be automatically adjusted from 0KPa to 8.62KPa and the absorption rate of elements is improved. Without sacrificing the spheroidizing level and mechanical properties, the amount of added nodularizer can be decreased by 0.2%-0.5%. Saving the costs and protecting the environment.
Decolorization of Azo Dyes by a New Strain CD-2 Isolated from the Textile Dye Contaminated Water
Authors: Cui Dai Zong , Zhao Min , Li Guo Fang , Gu Xiao Xu , Du Guang Ying Hui , Wang Chun Lei
Published: January 2011

In this study, a new strain was isolated by us based on its efficiency to decolorize azo dyes. Identification of this isolate by 16S rDNA technique revealed that the strain belonged to Escherichia, and clustered within Escherichia coli. According to this, we renamed our strain as E. coli CD-2. The strain CD-2 could decolorize azo dyes effectively under aerobic conditions. CD-2 exhibited good decolorization ability in the pH range from 3 to 11, temperature from 30°C to 42°C and salinity from 1% to 4%. CD-2 could decolorized different azo dyes (methyl red, Congo red, eriochrome black T and eriochrome red B) within 16h, and the decolorizing rate were 97.15%, 86.03%, 56.92% and 81.14%, respectively. This degradation potential increased the applicability of this strain for the azo dye removal.
The Study of Phosphorus Removal by Electrocoagulation with Iron-Anodes
Authors: Lin Yong Bo , Yang Yang , Wang Shuai
Published: January 2011

Determined to adopt iron as anodes, and Ti-base board with coating as cathodes. To optimize the reaction conditions of phosphorus removal by electrocoagulation (EC), testing the effect of current density, electrode distance, initial pH and electrolysis time on the phosphorus removal. According to the results, the optimal conditions for the phosphorus removal in the EC treatment were obtained, i.e., 20 mA/cm2 of current density, 2cm of distance and 10min of reaction time were optimum. Under these conditions, phosphorus removal by electrocoagulation reached to 95.07%, 10min later the change of total phosphorus (TP) removal rate is not obvious. By the end of this test, phosphorus removal by electrocoagulation reached to 99.68%.
Study on the Co-Migration of Pb with Soil Colloids under Rainfall
Authors: Shang Shu Bo , Li Xu Qian
Published: January 2011

In this experiment, clay loam, fine sandy loam and silty loam collected from the farmland (FL), floodplain (FP) and Loess platform (LP) in Changchun separately were chosen to study the role of rainfall on the migration of heavy metal (Pb)-colloid complexes quantitively through soil column simulation in laboratory. The purpose of this study is to illuminate the tendency for colloid-Pb co-migrating to deep soil and groundwater. The study shows whether how the mechanism between colloid and heavy metal acts, the processes exist in common. First, through some sort of adsorption, heavy metal form complexes with colloids, and co-migrate in the underground environment, which is called a co-migration mechanism. And Pb migrated out the FL, LP, and FP soil column contain 28.4%、25.7% and 22.6% complexes separately. Second, the heavy metal complexes with colloids have a certain stability and mobility. Third, in migrating process, the presence of colloids increases the migration concentration and amount of heavy metal (Pb). And the quantitative of Pb migrated out the FL, LP and FP soil column which added colloids are 1.37、1.36 and 1.25 double control columns separately. Forth, the presence of colloids reduces the reduction ability heavy metal (Pb), and shortens the consumption time for heavy metal (Pb) migrating to a certain depth of groundwater. And the time consuming of Pb migrating to groundwater with same deep is Loess horizon>floodplain horizon>farmland horizon.
Antioxidant Properties of Various Extracts from Laoying Tea (Litsea coreana L.)
Authors: Ji Hong Fang , Zhang Ling Wen , Zhang Hai Yan , Yang Ming Duo , Li Jian
Published: January 2011

Laoying Tea, leaves of Litsea coreana L., is conventionally consumed as healthy tea beverage in southern China for hundreds of years. The ethanolic, cold water and hot water extracts were prepared and their antioxidant properties studied. At 160 µg/mL, the cold water extract showed a high reducing power of 1.056. With regard to the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals, extracts were effective in the order: ethanolic > cold water > hot water extracts. EC50values for chelating ability on ferrous ions for ethanolic, cold water and hot water extracts were 10.23, 0.85 and 1.12 mg/mL, respectively. Total phenols were the major naturally occurring antioxidant components found and were in the order: cold water > hot water > ethanolic extracts. Based on EC50values, the various extracts from laoying tea were effective antioxidants.
The Deflection Angle Impact on the Oil Containment by Boom Based on the Numerical Wave Flume
Authors: Feng Xing , Wu Wan Qing , Zhang Feng Li
Published: January 2011

The 2-D numerical wave flume, based on the Reynolds Averaged Navier-Stokes equations and the standard turbulence model, was developed to simulate the turbulent flows with the free surface, in which the volume of fluid (VOF) method was used to handle the large deformation of the free surface and the relaxation approach of combined wave generation and absorbing was implemented, utilizing the commercial CFD software-FLUENT. The relationship of the wave parameters, initial failure velocity and the deflection angle was investigated.
Effects of pH and Aeration on the Production of Docosahexaenoic Acid by Thraustochytrium aureum in Controlled Batch Fermentor Cultures
Authors: Yang Ge , Yan Liu , Gao Ming
Published: January 2011

Docosahexaenoic acid (DHA. C22:6 ω-3) is an important structural component of neural and retinal tissues .The DHA yield was highest at pH5.5. Glucose and maltose utilization were similar as a function of pH, linseed oil consumption increased at pH 5.5, indicating that the formation of DHA from linseed oil at pH5.5 was of increased importance. Increased aeration resulted in doubling of T. aureum cell dry weights, increasing DHA yields and increasing in the maximum DHA -specific production rate .
The Change Trends and Relations of Climatic and Hydrological Factors in the Liu River, Zhangwu County, Liaoning Province, China
Authors: Huang Jing , Wu Xiang Yun , Yan Han
Published: January 2011

Understanding of the interactions between climatic and hydrological factors is very important for both real situation analyses and predicting the climatic trends and potential hydrological consequences. Air temperature, precipitation, evaporation, discharge, sandiness and sediment transport for the period 1968-2009 were selected to discover the change trends and correlations of climatic and hydrological factors in the Liu River within the boundaries of Zhangwu county. The intra-year variation scope of air temperature and sandiness were largest and smallest respectively. Monthly change trend of every factor was very similarly except evaporation. The variation scope of inter annual sediment transport and evaporation were largest and smallest respectively. The trend of air temperature series for the period 1968-2009 was positive (p<0.01), and the trends of evaporation, discharge, sandiness and sediment transport series for the period 1968-2009 were negative (p<0.01).There were significant correlations among climatic and hydrological factors.
Effect of the Extent of Enzymatic Hydrolysis on Foaming and Emulsifying Properties of Corn Germ Protein Hydrolysates
Authors: Zhang Ming Di , Pang Yong , Xu Zhi Jun
Published: January 2011

The effect of the extent of the enzymatically modified corn germ protein isolates on foaming and emulsifying properties was investigated. The experimental results indicated that enzymatic treatment improved the FC and EA of corn germ protein isolates, but the ESI and FS were decreased. The EA and FC of samples were increased with DH improvement at low DH (<10.4%), and the ESI and FS were decreased when DH was enhanced. The extent of modification should be determined by the intended application of new food ingredient.
Study on the Toxicity and Key Toxicant of Coated White Board Effluence
Authors: Zhao Yu , He Yi Xin , Xue Guo Xin , Lei Xiao Chun , Tang Yan Jun , Zhang Yong
Published: January 2011

With the continuous improvement of environmental protection requirements, the discharge of secondary fiber industry including waste water, gas and sludge were controlled more strictly. The toxicity studies on coated white board effluents of secondary fiber were carried out by using optimized photo bacteria method. The results showed that the value of EC50 for acute toxicity in coated white board effluents, which were produced from liners effluents and integrated effluents, were 85.50% and 89.77% respectively, reached the level of Moderately Toxic, by contraries from barrier coating effluents, was 157.57%, belong to Non-Toxic Class. Therefore, it should be pay more attention to the toxicity and toxicity emission load of coated white board effluents. Additionally, it can be seen from the analysis of organic compositions that key toxicant produced by liners effluents was diethyl phthalate. And there were no key toxicants of barrier coating effluents.
A Novel PCR Site-Directed Mutagenesis to Modify Structure of Erabutoxin B
Authors: Wu Fang Hui , Li Yan Jun , Yang Ting Song , Ying Tian Yi
Published: January 2011

Objective: A convenient PCR was used for site-directed mutagenesis to modify structure of proteins or peptides, For function-structure studies of proteins or peptides. Method: the one-step PCR site-directed mutagenesis strategy can introduce mutation of gene through 5/-end of primer, which was applied on peptide Erabutoxin B (EB) to produce three mutants of EB, the activity of mutants was detected by LD50 value of mice. Result: S8Y, R33D, K47R three mutants of EB were obtained by one-step PCR, LD50 of mutants indicated that the activity of mutants decreased in different degree, the activity of R33D was nearly deprived. Conclusion: one-step PCR site-directed mutagenesis was convenien and efficient, it can be applied on restructuring the primary structure of proteins or peptides.
Comparison of the Physicochemical Properties of Red Bean and Mung Bean Starch
Authors: Han Chun Ran , Yang Ming Duo , Xu Xin , Ma Yong Qiang
Published: January 2011

Differences in physicochemical properties of starch isolated from red bean and mung bean, including chemical composition, amylose content, starch granule morphology, swelling power and solubility, thermal properties and freeze-thaw stability were compared. There was no significant difference on the chemical compositions between the two starches. Most of the granules of red bean starch was oval or elliptical, the diameter was between 9.54 μm-30.98μm, the granules from mung bean starch was round or oval, the diameter was between 10.50 μm-27.59μm; The extent of solubility in mung bean starch was signiﬁcantly higher than red bean starch between 60 and 90°C, the temperature that the swelling power began to increase obviously of mung bean starch was higher than that of red starch at 60 to 90°C. To, Tp, TcandΔH of red bean starch were higher than that of mung bean.
Steam Explosion Pretreatment and Ethanol Extraction of Wheat Straw
Authors: Hui Lan Feng , Zhao Ting , Si Chuan Ling , Liu Zhong
Published: January 2011

It has been increasing the interest in the study of alternative pulping processes that conciliate high performance and protection to the environment. In the same way, the use of agricultural residues has been more and more reminded as promising raw material for production of bioethanol and chemical products. The wheat straw is a very abundant agricultural residue and it has been used in many countries for the production of cellulosic pulps. This work describes the effect of the pretreatment by steam explosion on the ethanol-water extraction of wheat straw. The samples of wheat straw were treated by steam explosion at 190°C and 210 °C for 4 minutes and later transferred to the treatment with ethanol-water (1:1, v:v) at different temperatures (140, 160 and 200 °C) and 60 minutes as reaction times. The pretreatment led to an increase in the amount of extracted lignin in the ethanol pulping. This effect was more pronounced in the pulping performed at lower temperatures. The use of higher temperatures in the treatment by steam explosion was more significant as shown by lower pulp yield and higher extractive extent.
Expression and Functional Analysis of BjMT-2, a Metallothionein Type-2 from Brassica juncea, in E. coli
Authors: Song Yu Zhen , Li Ping Ping , Du Ye Jie
Published: January 2011

The expression and functional analysis of plant metallothionein type 2 from Brassica juncea were achieved in E. coli. The BjMT-2 cDNA was cloned into the pETM-20 vector fused to the Thioredoxin gene and induced to E. coli. Northern blot and SDS PAGE analysis showed, respectively, that the transcription of the TrxA::BjMT-2 fusion gene and the expression of the fusion protein were predominately in E. coli cells after IPTG induction. The BjMT-2 in the crude extraction prepared from the E. coli cells bearing TrxA::BjMT-2 vector were reacted with the antibody directed against BjMT-2. The E. coli cells exhibited an increased tolerance against Cu2+ and Cd2+ after BjMT-2 expression.
Investigation of Rural Biogas Project in Northern Part of China
Authors: Shi Yue , Chen Chang Ping , Sun Xiang Wen
Published: January 2011

Problems associated with non-sustainable use of fossil fuels have led to increased awareness and widespread research into the accessibility of new and renewable energy resources. As the renewable energy, biogas should be widely used for its advantages in environmental friendly, security and efficiency. But factors in policy and environment made the utilization of biogas hard to achieve in cold areas. This article focus on the problem, make conclusions and give advices based on the analysis of the example of China’s rural areas in the north. The exact information was obtained via questionnaire survey, aiming at promoting the utilization of biogas in cold areas.
Analysis on the Environment Conservation Value and Purification Capacity of Wetland Plants
Authors: Liu Yin Ge , Wen Yan Jun
Published: January 2011

The environmental conservation values of wetland plants were estimated in successive terms, by using the calculation model of ecosystem service values. The removal experiments of CODcr and TN in wastewater were carried out. Cattail, reed, small sedge and potamogeton were chosen as experimental wetland plants, while vertical flow wetland was constructed by using flyash concrete as filling material. The results show that the environmental protection function of wetland is remarkable. Of the whole ecosystem service values of the wetland plants, the most is the pollutant purification value accounting for 28.95%, while the least is the raw food production value accounting for 0.11%-0.48%. Cattail has the greatest purification capacity of CODcr in wastewater with the removal rate of 42.64%, potamogeton has the greatest purification capacity of TN with the removal rate of 62.83%, while reed has pretty good purification capacity of both CODcr and TN. The pollutant removal rates of the submerged plants increase with temperature rises.
On Control Regulation of Urban Initial Period Rainwater Pollution
Authors: You Xue Yi , Li Jing , Li Yan Wei , Ji Min , Wang Xiu Duo , Zhao Le Jun , Pan Liu Ming
Published: January 2011

The initial period rainwater has a great influence on the quality of surface water in Tianjin city. To reducing the initial period rainwater pollution, the effective control measures must be taken for initial period rainwater. A comprehensive operation regulation by using the surplus capacity of sewage treatment plant, reservoirs and rivers is proposed and studied in this paper. The order of initial period rainwater storage is firstly in sewage treatment plant, then in reservoirs and finally drainage to rivers. By carefully arranging the intercepted amount of every storage stage, the pollution of initial period rainfall in an example Tianjin area is shown to be effectively solved.
Cloning Na+/H+ Antiporter Gene (nhaA) and Analysis of Function in Soybean
Authors: Wang Quan Wei , Chen Liang , Zhang Hai Ling
Published: January 2011

The osmotic stress and ion toxicity caused by high salt is one of the major abiotic stress factors that affect the crop growth and development. Na+/H+ antiporter is the key factor in the salt-stress tolerance in organism. It can maintain normal growth and metabolism of organism under high salt stress. PCR was per- formed to clone a plasma membrane Na+/H+ antiporter gene nhaA from Pseudomonas stutzeri, and the plant expression vector pBI121-nhaA was constructed. NhaA gene was trans- formed into soybean growing point via Agrobacterium- mediated transformation. PCR, Southern blot analysis showed that nhaA gene had been integrated into the soybean genome. Salt resistance analysis showed that the relative electronic conductivity of the transformed plants plasma membrane was signify- cantly lower than that of the control under salt stress. While the content of chlorophyll and proline in the transformed plants were significantly higher than that in the controls. The expression of nhaA gene increased the salt stress tolerance of the transgenic soybean and provided excellent candidate genes for improving salt tolerance of crops.
The Mixed Bacteria Trichoderma Optimized Conditions
Authors: Han Song , Li Guo Qiang , Guo Si Yao , Sun Jin Bing , Wang Feng Lu , Wang Ying Wei , Jiang Li , He Dong Po
Published: January 2011

In this paper, we researched the activity of the trichoderma viride and trichoderma koningi, and measure the enzyme activity of carboxymethyl cellulose (CMC) and filter paper activity (FPA) of them. And then, mixed trichoderma viride and trichoderma koningi to determine the optimal conditions, which according to influence of the revolutions, the layer of gauze, temperature. It turned out that the effective conditions was: 150r/min of revolutions, 3 layers of gauze, two wafer with 2 cm in diam of inoculum size and 30°C.
Anti-Fungal Activity Characterization of Four Rosinyl Quaternary Ammonium Salts
Authors: Liang Tao , Li Jian , Li Shu Jun , Jin Yan
Published: January 2011

The anti-fungal activities of four self-prepared rosinyl quaternary ammonium salts (QAS), QASA, BQAS1, BQAS2 and DQAS, were evaluated with paper disc method in this paper. The result showed that, all the QAS of rosin were bioactive to the selected fungi, Irpex lacteus, Trametes versicolor, Phanerochaete chrysosporium, Gloeophyllum trabeum, Chaetomium globosum, and Paecilomyces variotii. There were obvious inhibition halos for all QAS to the fungi. The result proved that the QAS of rosin were broad-spectrum anti-fungal. Especially, to white rot fungus Trametes versicolor, brown rot fungus Gloeophyllum trabeum and soft rot fungus Chaetomium globosum, they were more effective. When the concentration reached 3.2% (w/w), the inhibition halo diameters all met or exceeded 25mm. Among these four anti-fungal derivatives of rosin, the Gemini QAS (BQAS1 and BQAS2) had bigger inhibition halo diameters than the others, which indicated that the Gemini QAS of rosin were more anti-fungal than the normal QAS of rosin.
Kinetics of Monochloramine Decay in Model and Distribution System Waters
Authors: Liu Shao Gang , Fan Chen Feng , Zhu Zhi Liang
Published: January 2011

The model of second-order reaction kinetics has been used to stimulate the monochloramine decay in model and distribution system waters by nonlinear fit. Several factors were investigated, including pH, bromide, nitrite concentrations in this system. The results showed that pH value was an important factor on the monochloramine decay rate, especially when pH was below 7.0. Presence of bromide ions had different impact under experimental conditions However, when pH was above 7.60, 0.1 mg/L concentration of bromide affect hardly the decay rate of mononchloramine. The results demonstrated that the second-order kinetic model could fit well the experimental results of monochloramine decay reaction under the conditions of bromide, and nitrite ion. Finally, the work presented here validates and extends this model for use in distribution systems under realistic chloramination conditions.
Isolation and Dye Decolorization of a Bacillus subtilis Strain LS02 Exhibiting Laccase Activity
Authors: Du Mei Hui , Zhao Min , Lu Lei , Wang Tian Nv , Li Tai Lun , Zhao Li Yan , Pan Jun Bo , Li Guo Fu , Li Jun
Published: January 2011

A newly isolated strain LS02 was estimated for its ability in dye decolorization. The LS02 strain was identified as Bacillus subtilis by the combination of physicochemical tests and 16S rDNA sequence analysis. The isolated strain could oxidize the laccase substrate syringaldazine, indicating the existence of laccase activity. B. subtilis LS02 grown well in the pH range of 5.0~9.0, and showed an optimum growth temperature at 37°C. Indigo carmine could be completely decolorized by B. subtilis LS02 after 4 days, whereas Remazol Brilliant Blue R, reactive black 5 and crystal violet were poorly decolorized. The result indicated that the laccase of B. subtilis LS02 may be suitable for the application in textile bleaching of indigo carmine.
Optimal Location of Auto-Control Valves in Water Distribution Networks
Authors: Li Xia , Li Guo Jin , Zhao Xin Hua , Zhang Li
Published: January 2011

In recent years, the SCADA (supervisory control and data acquisition) systems are applied widely for water distribution networks, where the auto-control valves are used to reduce the excessive water pressure of each node for further leakage control. In this paper, first the existing optimal location models of valves are briefly introduced, whose shortcomings are proposed. Then a new method based on direct hydraulic analysis theory of the mass conservation of nodes and the energy conservation of loops is available for optimal location of auto-control valves. Finally taking the water network of Tianjin Port Free Trade Zone as an example, valve location problem is illustrated, which shows the feasibility of the method.
Effects of Substrate Concentration and pH on H2-Producing Capacity with Clostridium R33 sp.nov.
Authors: Yang Jian Yu , Pan Xin Yu , Zhang Jing Wei , Han Wei , Li Yong Feng
Published: January 2011

Improving bio-hydrogen-producing capacity is the key to realize industrialization for the H2-producing technology. One of the most effective approaches is to investigate the factors that affect the H2-producing capacity. Different glucose concentrations and pH were designed in anaerobic Clostridium R33 sp.nov. cultures. The hydrogen production varies according to glucose concentration and pH, respectively. It is founded the optimal glucose concentration is 12.5 g/L and the optimal pH is about 4.0.
Study on Reversibility of Genetic Toxicity of TDI in Mice
Authors: Yu Miao , Ji Yu Bin
Published: January 2011

Study on whether genetic toxicity of toluene diisocyanate (TDI) is reversible. This paper detected Chromosomal aberrations and content of RNA & DNA. Chromosome aberration rate and the RNA/DNA ratio of TDI 1/4LC50 and 1/2 LC50 dosing exposure group were higher than negative control group significantly (P<0.01). There was no significant difference between 1/4LC50 and 1/2LC50 (P>0.05).The results showed that the damage of TDI on chromosomes and DNA was repairable, but can not be repaired completely.
Chemical Composition and Antibacterial Activity of Essential Oil from the Basil Cultivated in Heilongjiang
Authors: Lu Zhan Guo , Wang Lin , Li Wei
Published: January 2011

The essential oil was obtained from the white Basil (Ocimum basilicum L.) grown in Heilongjiang by hydrodistillation in a clevenger apparatus with 0.28% yield. Then the chemical composition of the essential oil was analyzed by GC-MS. A total of 25 constituents were detected, 24 compounds representing for 98.99% were identified, of which the most abundant compound was β-linalool (64.421%). The antibacterial activities of oil were tested against 3 different general of bacteria and were compared with that of the Penicillin sodium, using the filter paper disc agar diffusion method. The result showed that the oil has powerful inhibitory effects against Staphylicoccus albus, followed by Bacillus subtilis, Escherichia coli.
Taurine Induces the Apoptosis in Pulmonary Artery Smooth Muscle Cell
Authors: Zhang Xiao Dan , Sheng Jie Jing , Zhao Feng Hua , Zhu Da Ling
Published: January 2011

As an endogenous anti-damage material in organism, Taurine (Tau) stems from food and almost resides in all animals’ cells by means of liberation，which could be found in diverse biological actions, including protection against ischemia-reperfusion injury, modulation of intracellular calcium concentration, and antioxidant, and blood pressure-lowering effects[1]. It also has an effect that inducing apoptosis in certain kinds of cells. Our results showed that Tau can degrade cell viability, mitochondrial potentials, and the expression of Procasepase-9 in PASMCs. In conclusion, Tau induces apoptosis in PASMCs through mitochondrial-dependent pathway.
The Isolation, Identifition and Fermentation of Bacillus for Degradation of Terephthalic Acid
Authors: Zhang Wei Ling , Zhang Jian Fei , Li Zheng , Gong Ji Xian
Published: January 2011

Terephthalic acid is one of the main contaminations released by PET processing and it's toxic to environment and animals. Useful microbe must be found out to biodegrade it. Bacillus came from waste water may be powerful to biodegrade it, so the waste water were used as the sources of bacillus. The microbe was enriched in enrichment medium first and then isolated on selective medium containing terephthalic acid as the only carbon source to get the microbe suitable for degradation. The purified microbe was used to biodegrade terephthalic acid at different temperature to find out its suitable living and degradation condition. The microbe was identified with molecule biological identification and biolog express identification. The fermentation condition of the purified microbe was studied. The enzyme activity, growth condition and degradation condition was studied. The results showed that the isolated microbe was a kind of bacillus named comamonas testosteroni. It's powerful to degrade terephthalic acid at 37 °C with the rotational speed of 200 rpm. The pH of the medium would increase during degradation of substrate. The degradation would consumed a lot of oxygen. A degradation path may be deduced.
The Treatment of Acid Copper-Containing Wastewater by Eggshell-Flyash System
Authors: Liu Tao
Published: January 2011

Removal efficiency of heavy metal Cu from acid wastewater using egg shell as adsorbent was investigated. The influence of calcination temperature and dosage of egg shell, as well as pH value on the removal efficiency were discussed to obtain the optimum removal condition. The results show that the removal rate of lead would achieve the best result of 99.56% when we use the egg shells calcined at 400°C,2h, and the content of egg shells is 30g/L, pH=3.0.Using eggshells to treat acid chrome-containing wastewater is simple and easy to be operated, which has application prospects for the process and good treatment effect.
Design and Research on New Efficient Clarification Tank Treating Raw Water of Low-Temperature and High-Color
Authors: Sun Nan , Yu Shui Li , Ma Li Xin , Xu Xiao Bei , Yi Xue Song
Published: January 2011

Based on advantages of plated sedimentation tank, mechanical stirring clarification tank, Densadge, design new efficient clarification tank treating raw water of low-temperature and high-color. Its functions of mixture, flocculation, precipitation and sludge concentration are gather in integral whole. This paper introduces mainly the tank structures, characteristics of technology, design principle, application parameters and operating conditions treating raw water of low-temperature and high-color, laboratory researches show that the tank possess the following characteristics: small occupation area, high load, uniform water distribution, reasonable flow trend, owning good adaptability and feasibility treating raw water of low-temperature and high-color, high removal rate of colour and turbidity has reached to high standards.
Design of an Intelligent Bean Sprout Incubator Based on MSP430 SingleChip Computer
Authors: Huang Han Ying , Yin Tao , Zhao Si Ming , Xiong Shan Bo , Li Xiao Yu , Du Jun Mei , Cao Jian Chao
Published: January 2011

At present, there exist a lots of problems in general bean sprout incubators such as uneven spray, water wasting, slow heating, high energy consumption, inaccurate temperature control and so on. To solve these problems, an intelligent bean sprout incubator has been developed based on MSP430 Single Chip Computer. The incubator has advantages of humidity uniformity, water saving, fast heating, low energy consumption, and precise temperature control.