bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantitative and Compositional Study of Monospecies Biofilms of Spoilage Microorganisms in the Meat Industry and Their Interaction in the Development of Multispecies Biofilms
Authors: Ripolles-Avila Carolina , García-Hernández Nerea , Cervantes-Huamán Brayan H. , Mazaheri Tina , Rodríguez-Jerez José Juan
Published: December 2019

Food spoilage is a serious problem in the food industry, since it leads to significant economic losses. One of its main causes is the cross-contamination of food products from industrial surfaces. Three spoilage bacterial species which are highly present in meat and the gastrointestinal tract of chickens were selected: Pseudomonas fragi, Leuconostoc gasicomitatum, and Lactobacillus reuteri. The dual aim was to determine their ability to form monospecies biofilms and to examine how they interact when they coexist together. To do so, mature monospecies biofilms were produced statically for seven days at a temperature of 30 °C. L. gasicomitatum was also used to investigate the behavior of P. fragi and L. reuteri in the formation of multispecies biofilms. The structure and composition of the monospecies biofilms were evaluated by direct epifluorescence microscopy, and the multispecies biofilms were evaluated by plate counting. Both L. gasicomitatum and L. reuteri were able to form biofilms, with counts of approximately 7 Log CFU/cm2 and a defined structure. However, P. fragi obtained counts to the order of 4 Log CFU/cm2, which is significantly different from the previous species (P < 0.05), and it had no network of cell conglomerates. The content of the L. gasicomitatum and L. reuteri biofilm matrices were 70–80% protein, unlike P. fragi, which presented a higher polysaccharide content (P < 0.05). In the multispecies biofilms, the presence of P. fragi did not affect the growth of L. gasicomitatum, which remained at between 5.76 to 6.1 Log CFU/cm2. However, L. reuteri was able to displace L. gasicomitatum growth after 24 h of coexisting in a mixed biofilm, presenting differences in counts of approximately 2 Log CFU/cm2. The study of the biofilms constructed by food industry resident microbiota can help to understand the ecological relations that exist between species, characterize them, and propose strategies to eliminate them. The name of genes and species should be written in italic.
The Conifer Root and Stem Rot Pathogen (Heterobasidion parviporum): Effectome Analysis and Roles in Interspecific Fungal Interactions
Authors: Wen Zilan , Zeng Zhen , Ren Fei , Asiegbu Fred O.
Published: December 2019

Heterobasidion parviporum Niemelä & Korhonen is an economically important basidiomycete, causing root and stem rot disease of Norway spruce (Picea abies (L.) Karst) in Northern Europe. The H. parviporum genome encodes numerous small secreted proteins, which might be of importance for interacting with mycorrhiza symbionts, endophytes, and other saprotrophs. We hypothesized that small secreted proteins from H. parviporum (HpSSPs) are involved in interspecific fungal interaction. To identify HpSSP-coding genes potentially involved, we screened the H. parviporum effectome and compared their transcriptomic profiles during fungal development and in planta tree infection. We further conducted phylogenetic analysis, and identified a subset of hypothetical proteins with nonpredicted domain or unknown function as HpSSPs candidates for further characterization. The HpSSPs candidates were selected based on high-quality sequence, cysteine residue frequency, protein size, and in planta expression. We subsequently explored their roles during in vitro interaction in paired cultures of H. parviporum with ectomycorrhizal Cortinarius gentilis, endophytic Phialocephala sphaeroides, saprotrophs (Mycena sp., Phlebiopsis gigantea, and Phanerochaete chrysosporium), respectively. The transcriptomic profile revealed that a large proportion of effector candidates was either barely expressed or highly expressed under all growth conditions. In vitro dual-culture test showed that P. sphaeroides and C. gentilis were overgrown by H. parviporum. The barrage zone formation or no physical contact observed in paired cultures with the saprotrophs suggest they had either combative interaction or antibiosis effect with H. parviporum. Several HpSSPs individuals were up- or downregulated during the nonself interactions. The results of HpSSPs gene expression patterns provide additional insights into the diverse roles of SSPs in tree infection and interspecific fungal interactions.
Kaolinite-Magnesite Based Ceramics. Part I: Surface Charge and Rheological Properties Optimization of the Suspensions for the Processing of Cordierite-Mullite Tapes
Authors: Hammas Aghiles , Lecomte-Nana Gisèle , Azril Nadjet , Daou Imane , Peyratout Claire , Zibouche Fatima
Published: December 2019

The present study aimed at investigating the influence of the concentration of sodium silicate and sodium hexametaphosphate on the dispersion of an aqueous kaolinitic clay slurry regarding further use for the tape casting process. The zeta potential of the kaolinitic clay slurry matched the requirements for tape casting. The addition of magnesite in the kaolinitic slurries tended to increase the zeta potential towards the required limit values. Despite this, the further addition of surfactants allowed improving the zeta potential in agreement with the tape casting conditions. Accordingly, the rheological behavior, under continuous and oscillatory flow conditions, of various mixtures of magnesite and a kaolinitic clay was studied. Regarding the pH and the zeta potential measurements, the E–F attraction prevailed at low pH value, and F–F or E–E attraction was predominant at high pH value. All slurries exhibited a shear thinning behavior, which was well-correlated by the Herschel–Bulkley model. It appeared that the best stability for the kaolinitic clay slurries was obtained while using 0.4 mass% and 1.2 mass% of sodium hexametaphosphate and sodium silicate, respectively. An increase in the magnesite concentration above 6 mass% led to a complex behavior with low cohesion energy due to the occurrence of soluble complexes.
Anti-Inflammatory and Anticancer Properties of Bioactive Compounds from Sesamum indicum L.—A Review
Authors: Wu Ming-Shun , Aquino Levent Bless B. , Barbaza Marjette Ylreb U. , Hsieh Chieh-Lun , De Castro-Cruz Kathlia A. , Yang Ling-Ling , Tsai Po-Wei
Published: December 2019

The use of foodstuff as natural medicines has already been established through studies demonstrating the pharmacological activities that they exhibit. Knowing the nutritional and pharmacological significance of foods enables the understanding of their role against several diseases. Among the foods that can potentially be considered as medicine, is sesame or Sesamum indicum L., which is part of the Pedaliaceae family and is composed of its lignans such as sesamin, sesamol, sesaminol and sesamolin. Its lignans have been widely studied and are known to possess antiaging, anticancer, antidiabetes, anti-inflammatory and antioxidant properties. Modern chronic diseases, which can transform into clinical diseases, are potential targets of these lignans. The prime example of chronic diseases is rheumatic inflammatory diseases, which affect the support structures and the organs of the body and can also develop into malignancies. In line with this, studies emphasizing the anti-inflammatory and anticancer activities of sesame have been discussed in this review.
Hypotonic, Acidic Oxidizing Solution Containing Hypochlorous Acid (HClO) as a Potential Treatment of Hailey-Hailey Disease
Authors: Cialfi Samantha , Calabro Salvatore , Franchitto Matteo , Zonfrilli Azzurra , Screpanti Isabella , Talora Claudio
Published: December 2019

Hailey–Hailey disease (HHD) is a rare, chronic and recurrent blistering disorder, characterized by erosions occurring primarily in intertriginous regions and histologically by suprabasal acantholysis. Mutation of the Golgi Ca2+-ATPase ATP2C1 has been identified as having a causative role in Hailey–Hailey disease. HHD-derived keratinocytes have increased oxidative-stress that is associated with impaired proliferation and differentiation. Additionally, HHD is characterized by skin lesions that do not heal and by recurrent skin infections, indicating that HHD keratinocytes might not respond well to challenges such as wounding or infection. Hypochlorous acid has been demonstrated in vitro and in vivo to possess properties that rescue both oxidative stress and altered wound repair process. Thus, we investigated the potential effects of a stabilized form of hypochlorous acid (APR-TD012) in an in vitro model of HHD. We found that treatment of ATP2C1-defective keratinocytes with APR-TD012 contributed to upregulation of Nrf2 (nuclear factor (erythroid-derived 2)-like 2). Additionally, APR TD012-treatment restored the defective proliferative capability of siATP2C1-treated keratinocytes. We also found that the APR-TD012 treatment might support wound healing process, due to its ability to modulate the expression of wound healing associated cytokines. These observations suggested that the APR-TD012 might be a potential therapeutic agent for HHD-lesions.
Peptide/Peptoid Hybrid Oligomers: The Influence of Hydrophobicity and Relative Side-Chain Length on Antibacterial Activity and Cell Selectivity
Authors: Frederiksen Nicki , Hansen Paul R. , Björkling Fredrik , Franzyk Henrik
Published: December 2019

Previous optimisation studies of peptide/peptoid hybrids typically comprise comparison of structurally related analogues displaying different oligomer length and diverse side chains. The present work concerns a systematically constructed series of 16 closely related 12-mer oligomers with an alternating cationic/hydrophobic design, representing a wide range of hydrophobicity and differences in relative side-chain lengths. The aim was to explore and rationalise the structure–activity relationships within a subclass of oligomers displaying variation of three structural features: (i) cationic side-chain length, (ii) hydrophobic side-chain length, and (iii) type of residue that is of a flexible peptoid nature. Increased side-chain length of cationic residues led to reduced hydrophobicity till the side chains became more extended than the aromatic/hydrophobic side chains, at which point hydrophobicity increased slightly. Evaluation of antibacterial activity revealed that analogues with lowest hydrophobicity exhibited reduced activity against E. coli, while oligomers with the shortest cationic side chains were most potent against P. aeruginosa. Thus, membrane-disruptive interaction with P. aeruginosa appears to be promoted by a hydrophobic surface of the oligomers (comprised of the aromatic groups shielding the cationic side chains). Peptidomimetics with short cationic side chains exhibit increased hemolytic properties as well as give rise to decreased HepG2 (hepatoblastoma G2 cell line) cell viability. An optimal hydrophobicity window could be defined by a threshold of minimal hydrophobicity conferring activity toward E. coli and a threshold for maximal hydrophobicity, beyond which cell selectivity was lost.
(R)-2-Phenyl-4,5-Dihydrothiazole-4-Carboxamide Derivatives Containing a Diacylhydrazine Group: Synthesis, Biological Evaluation, and SARs
Authors: Li Feng-Yun , Liu Jing-Bo , Gong Jia-Ning , Li Gen
Published: December 2019

A series of (R)-2-phenyl-4,5-dihydrothiazole-4-carboxamide derivatives containing a diacylhydrazine moiety were designed and synthesized. Their structures were confirmed by melting points, 1H NMR, 13C NMR, and elemental analysis (EA). Their antifungal and insecticidal activities were evaluated. The antifungal activity result indicated that most title compounds against Cercospora arachidicola, Alternaria solani, Phytophthora capsici, and Physalospora piricola exhibited apparent antifungal activities at 50 mg/L, and better than chlorothalonil or carbendazim. The EC50 values of (R)-N’-benzoyl-2-(4-chlorophenyl)-4,5-dihydrothiazole-4-carbohydrazide (I-5) against six tested phytopathogenic fungi were comparable to those of chlorothalonil. The CoMSIA model showed that a proper hydrophilic group in the R1 position, as well as a proper hydrophilic and electron-donating group in the R2 position, could improve the antifungal activity against Physalospora piricola, which contributed to the further optimization of the structures. Meanwhile, most title compounds displayed good insecticidal activities, especially compound (R)-N’-(4-nitrobenzoyl)-2-(4-nitrophenyl)-4,5-dihydrothiazole-4-carbohydrazide (III-3). The insecticidal mechanism results indicated that compound III-3 can serve as effective insect Ca2+ level modulators by disrupting the cellular calcium homeostasis in Mythimna separata.
CX-5461 Inhibits Pancreatic Ductal Adenocarcinoma Cell Growth, Migration and Induces DNA Damage
Authors: El Hassouni Btissame , Mantini Giulia , Immordino Benoît , Peters Godefridus J. , Giovannetti Elisa
Published: December 2019

Background: Inhibition of ribosome biogenesis has recently emerged as a promising strategy for the treatment of metastatic tumors. The RNA polymerase I inhibitor CX-5461 has shown efficacy in a panel of cancer types and is currently being tested in clinical trials. However, further preclinical studies to unravel molecular mechanisms underlying the activity of this drug are warranted. Methods: In this study, we have investigated the effects of CX-5461 on cell growth and migration of pancreatic cancer cells by the sulforhodamine-B and wound healing assay, respectively. Furthermore, we assessed the expression of epithelial-to-mesenchymal transition (EMT) genes by qRT-PCR, while protein expression of DNA damage marker phospho-H2A.X was studied by Western blot and immunofluorescence. Results: CX-5461 inhibits pancreatic cancer cell growth in the nanomolar range and inhibits the migratory capability of the cells. Additionally, CX-5461 induced expression of EMT factor SNAI1 and caused DNA double-strand breaks as measured by increased expression of phospho-H2A.X. Conclusion: This study demonstrated that CX-5461 is active against pancreatic cancer cells and modulation of EMT factors, as well as increased expression of phospho-H2A.X, support further pre-/clinical investigations, including the analyses of these markers.
Beneficial Metabolic Effects of Chitosan and Chitosan Oligosaccharide on Epididymal WAT Browning and Thermogenesis in Obese Rats
Authors: Wang Jin , He Wanping , Yang Di , Cao Hua , Bai Yan , Guo Jiao , Su Zhengquan
Published: December 2019

Many anti-obesity chemicals have been withdrawn from the market due to serious adverse reactions, and the researchers have turned their attention to low-toxic natural products. Previous studies have demonstrated that chitosan (CTS) and chitosan oligosaccharide (COS) were low-toxic natural products for the use of weight loss. However, it is still unclear whether CTS and COS have positive effects on the thermogenesis. In this study, CTS and COS significantly reduced the weight gain of rats without affecting food intake and effectively inhibited adipose tissue hypertrophy and hyperplasia. Consistently, CTS and COS significantly increased the thermogenic capacity of obese rats induced by high-fat diet (HFD) and increased the expression of browning genes and proteins (UCP1, PGC1α, PRMD16, and ATF2) in white adipose tissue (WAT) and brown adipose tissue (BAT). In vitro, COS inhibited the formation of mature adipocytes and increased the expression of browning genes. In conclusion, COS and CTS was used to explore the function and mechanism on thermogenesis, and CTS and COS can increase the browning of WAT and the thermogenesis of BAT to inhibit obesity. This effect may be achieved by promoting the expression of browning and thermogenic genes, providing new ideas for the utilization of COS and CTS.
Simultaneous Determination of Etomidate and Its Major Metabolite, Etomidate Acid, in Urine Using Dilute and Shoot Liquid Chromatography–Tandem Mass Spectrometry
Authors: Jung Yu-Kyung , You Soo Young , Kim Seon-Yeong , Kim Jin Young , Paeng Ki-Jung
Published: December 2019

Etomidate (ET) is a commonly used sedative-hypnotic agent such as propofol to induce anesthesia, and it is rapidly metabolized to etomidate acid (ETA) in liver. Herein, a simple method to determine ET and ETA in urine simultaneously was developed using liquid chromatography–tandem mass spectrometry (LC–MS/MS). A simple sample preparation method reduced the total analysis time. For all analytes, the separation was achieved in 6.5 min using reversed-phase chromatography with gradient elution. The best separation and detection of ETA was achieved using a porous graphitic carbon column. The column temperature was maintained at 30 °C to improve the efficiency and sensitivity. The calibration curves were linear over the concentration ranges of 0.4–120.0 ng/mL (ET) and 1.0–300.0 ng/mL (ETA), obtained with a weighting factor of 1/x2. The coefficients of determination (r2) were greater than 0.9958. The lower limits of quantification were 0.4 ng/mL (ET) and 1.0 ng/mL (ETA), intra-day (n = 6) and inter-day (n = 24) precision values for all compounds were less than 10.2% and 8.4%, respectively, while the intra- and inter-day accuracies were in the −9.9–2.9%, and −7.0–0.6%. The applicability of the method was examined by analyzing the urine samples obtained from ET users.
Fluorescence Polarization Immunoassay for Determination of Enrofloxacin in Pork Liver and Chicken
Authors: Shen Xing , Chen Jiahong , Lv Shuwei , Sun Xiulan , Dzantiev Boris B. , Eremin Sergei A. , Zherdev Anatoly V. , Xu Jianfa , Sun Yuanming , Lei Hongtao
Published: December 2019

Enrofloxacin (ENR) is a widely used fluoroquinolone (FQ) antibiotic for antibacterial treatment of edible animal. In this study, a rapid and highly specific fluorescence polarization immunoassay (FPIA) was developed for monitoring ENR residues in animal foods. First, ENR was covalently coupled to bovine serum albumin (BSA) to produce specific polyclonal antibodies (pAbs). Three fluorescein-labeled ENR tracers (A, B, and C) with different spacers were synthesized and compared to obtain higher sensitivity. Tracer C with the longest arm showed the best sensitivity among the three tracers. The developed FPIA method showed an IC50 (50% inhibitory concentration) of 21.49 ng·mL−1 with a dynamic working range (IC20–IC80) of 4.30–107.46 ng·mL−1 and a limit of detection (LOD, IC10) of 1.68 ng·mL−1. The cross-reactivity (CR) of several structurally related compounds was less than 2%. The recoveries of spiked pork liver and chicken samples varied from 91.3% to 112.9%, and the average coefficients of variation were less than 3.83% and 5.13%, respectively. The immunoassay took only 8 min excluding sample pretreatment. This indicated that the established method had high sensitivity, specificity, and the advantages of simplicity. Therefore, the proposed FPIA provided a useful screening method for the rapid detection of ENR residues in pork liver and chicken.
Soil Behaviour of the Veterinary Drugs Lincomycin, Monensin, and Roxarsone and Their Toxicity on Environmental Organisms
Authors: Li Peiyi , Wu Yizhao , Wang Yali , Qiu Jiangping , Li Yinsheng
Published: December 2019

Lincomycin, monensin, and roxarsone are commonly used veterinary drugs. This study investigated their behaviours in different soils and their toxic effects on environmental organisms. Sorption and mobility analyses were performed to detect the migration capacity of drugs in soils. Toxic effects were evaluated by inhibition or acute toxicity tests on six organism species: algae, plants, daphnia, fish, earthworms and quails. The log Kd values (Freundlich model) of drugs were: lincomycin in laterite soil was 1.82; monensin in laterite soil was 2.76; and roxarsone in black soil was 1.29. The Rf value of lincomycin, roxarsone, monensin were 0.4995, 0.4493 and 0.8348 in laterite soil, and 0.5258, 0.5835 and 0.8033 in black soil, respectively. The EC50 for Scenedesmus obliquus, Arabidopsis thaliana, Daphnia magna and LC50/LD50 for Eisenia fetida, Danio rerio, and Coturnix coturnix were: 13.15 mg/L,32.18 mg/kg dry soil,292.6 mg/L,452.7 mg/L,5.74 g/kg dry soil and 103.9 mg/kg (roxarsone); 1.085 mg/L, <25 mg/kg dry soil, 21.1 mg/L, 4.76 mg/L, 0.346 g/kg dry soil and 672.8 mg/kg (monensin); 0.813 mg/L, 35.40 mg/kg dry soil, >400 mg/L, >2800 mg/L, >15 g/kg dry soil, >2000 mg/kg (lincomycin). These results showed that the environmental effects of veterinary drug residues should not be neglected, due to their mobility in environmental media and potential toxic effects on environmental organisms.
Anti-Arthritic Activities of Supercritical Carbon Dioxide Extract Derived from Radiation Mutant Perilla Frutescens Var. Crispa in Collagen Antibody-Induced Arthritis
Authors: Jin Chang Hyun , So Yangkang , Kim Hyo-Young , Han Sung Nim , Kim Jin-Baek
Published: December 2019

We investigated the anti-arthritic effects of the radiation mutant Perilla frutescens var. crispa leaf extract (SFE-M) and wild type leaf extract (SFE-W), both prepared by supercritical carbon dioxide (SC-CO2) extraction, on collagen antibody-induced arthritis (CAIA) in Balb/c mice. Animals were randomly divided into four groups: control, CAIA, CAIA + SFE-M (100 mg/kg/day), and CAIA + SFE-W (100 mg/kg/day). The mice were subjected to the respective treatments via oral gavage once daily for 4 days. Mice treated with SFE-M developed less severe arthritis than the CAIA mice. They showed significantly improved arthritic score, paw volume, and paw thickness compared to the CAIA mice from days 3 through 7. Furthermore, histopathological analysis of ankle for inflammation showed that SFE-M treatment reduced inflammatory cell infiltration and edema formation. Similarly, the neutrophil-to-lymphocyte ratio (NLR) in the whole blood was 37% lower in mice treated with SFE-M compared with the CAIA mice. However, treatment with SFE-W did not result in any significant difference compared with the CAIA group. In conclusion, SFE-M treatment delays the onset of arthritis and alleviates its clinical manifestations in CAIA mice.
The Potential Synergistic Modulation of AMPK by Lippia citriodora Compounds as a Target in Metabolic Disorders
Authors: Olivares-Vicente Mariló , Sánchez-Marzo Noelia , Encinar José Antonio , Cádiz-Gurrea María de la Luz , Lozano-Sánchez Jesús , Segura-Carretero Antonio , Arraez-Roman David , Riva Catherine , Barrajón-Catalán Enrique , Herranz-López María , Micol Vicente
Published: December 2019

Lippia citriodora (LC) represents a complex plant-derived source of polyphenols and iridoids that has shown beneficial properties against obesity-related metabolic disorders. The complete extract and its major compound, verbascoside, have shown AMPK-activating capacity in cell and animal models. In this work, we aimed to elucidate the contribution of the different compounds present in the LC extract on the AMPK activation capacity of the whole extract. Semipreparative reversed-phase high-performance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry (RP-HPLC-ESI-TOF-MS) was used to identify the major compounds with bioassay-guided fractionation in an adipocyte cell model for the measurement of AMPK activity. Twenty-two compounds were identified and purified almost to homogeneity in 16 fractions, and three compounds, namely verbascoside, luteolin-7-diglucuronide and loganic acid, showed the highest AMPK-activating capacity. The synergy study using the checkerboard and fractional inhibitory concentration index (FICI) methods exhibited synergistic behavior between loganic acid and luteolin-7-diglucuronide. Molecular docking experiments revealed that these three compounds might act as direct agonists of AMPK, binding to the AMP binding sites of the gamma subunit and/or the different sites of the interaction zones between the gamma and beta subunits. Although our findings conclude that the bioactivity of the extract is mainly due to verbascoside, the synergy found between loganic acid and luteolin-7-diglucuronide deserves further research aimed to develop optimized combinations of polyphenols as a new nutritional strategy against obesity-related metabolic disorders.
Dietary Protein and Physical Activity Interventions to Support Muscle Maintenance in End-Stage Renal Disease Patients on Hemodialysis
Authors: Hendriks Floris K. , Smeets Joey S.J. , van der Sande Frank M. , Kooman Jeroen P. , van Loon Luc J.C.
Published: December 2019

End-stage renal disease patients have insufficient renal clearance capacity left to adequately excrete metabolic waste products. Hemodialysis (HD) is often employed to partially replace renal clearance in these patients. However, skeletal muscle mass and strength start to decline at an accelerated rate after initiation of chronic HD therapy. An essential anabolic stimulus to allow muscle maintenance is dietary protein ingestion. Chronic HD patients generally fail to achieve recommended protein intake levels, in particular on dialysis days. Besides a low protein intake on dialysis days, the protein equivalent of a meal is extracted from the circulation during HD. Apart from protein ingestion, physical activity is essential to allow muscle maintenance. Unfortunately, most chronic HD patients have a sedentary lifestyle. Yet, physical activity and nutritional interventions to support muscle maintenance are generally not implemented in routine patient care. To support muscle maintenance in chronic HD patients, quantity and timing of protein intake should be optimized, in particular throughout dialysis days. Furthermore, implementing physical activity either during or between HD sessions may improve the muscle protein synthetic response to protein ingestion. A well-orchestrated combination of physical activity and nutritional interventions will be instrumental to preserve muscle mass in chronic HD patients.
Dietary Bioactive Fatty Acids as Modulators of Immune Function: Implications on Human Health
Authors: Kumar Naren Gajenthra , Contaifer Daniel , Madurantakam Parthasarathy , Carbone Salvatore , Price Elvin T. , Van Tassell Benjamin , Brophy Donald F. , Wijesinghe Dayanjan S.
Published: December 2019

Diet is major modifiable risk factor for cardiovascular disease that can influence the immune status of the individual and contribute to persistent low-grade inflammation. In recent years, there has been an increased appreciation of the role of polyunsaturated fatty acids (PUFA) in improving immune function and reduction of systemic inflammation via the modulation of pattern recognition receptors (PRR) on immune cells. Extensive research on the use of bioactive lipids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and their metabolites have illustrated the importance of these pro-resolving lipid mediators in modulating signaling through PRRs. While their mechanism of action, bioavailability in the blood, and their efficacy for clinical use forms an active area of research, they are found widely administered as marine animal-based supplements like fish oil and krill oil to promote health. The focus of this review will be to discuss the effect of these bioactive fatty acids and their metabolites on immune cells and the resulting inflammatory response, with a brief discussion about modern methods for their analysis using mass spectrometry-based methods.
Iron as Therapeutic Target in Human Diseases
Authors: Gozzelino Raffaella , Poli Maura , Arosio Paolo
Published: December 2019

Iron is essential for almost all organisms, being involved in oxygen transport, DNA synthesis, and respiration; however, it is also potentially toxic via the formation of free radicals [...]
Dry Tablet Formulation of PLGA Nanoparticles with a Preocular Applicator for Topical Drug Delivery to the Eye
Authors: Ryu Woo Mi , Kim Se-Na , Min Chang Hee , Choy Young Bin
Published: December 2019

To enhance ocular drug bioavailability, a rapidly dissolving dry tablet containing alginate and drug-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles was proposed. For hygienic and easy administration of an accurate drug-dose with this tablet, the use of a preocular applicator was suggested. Herein, a dry tablet was prepared by embedding dexamethasone-loaded PLGA nanoparticles in alginate, which was deposited on the tip of the applicator. The nanoparticles were loaded with 85.45 μg/mg drug and exhibited sustained drug release for 10 h. To evaluate in vivo efficacy, dexamethasone concentration in the aqueous humor was measured after topical administration of the dry tablet, with the applicator, to rabbit eyes and was compared to that achieved with Maxidex®, a commercially-available dexamethasone eye drops. When applied with the preocular applicator, the dry tablet containing alginate could be fully detached and delivered to the eye surface. In fact, it showed up to 2 h of nanoparticle retention on the preocular surface due to tear viscosity enhancement, causing an estimated 2.6-fold increase in ocular drug bioavailability compared to Maxidex®. Therefore, the preocular applicator combined with a dry alginate tablet containing PLGA nanoparticles can be a promising system for aseptically delivering an accurate dose of ophthalmic drug with enhanced bioavailability.
Generation of Transgenic Self-Incompatible Arabidopsis thaliana Shows a Genus-Specific Preference for Self-Incompatibility Genes
Authors: Zhang Tong , Zhou Guilong , Goring Daphne R. , Liang Xiaomei , Macgregor Stuart , Dai Cheng , Wen Jing , Yi Bin , Shen Jinxiong , Tu Jinxing , Fu Tingdong , Ma Chaozhi
Published: December 2019

Brassicaceae species employ both self-compatibility and self-incompatibility systems to regulate post-pollination events. Arabidopsis halleri is strictly self-incompatible, while the closely related Arabidopsis thaliana has transitioned to self-compatibility with the loss of functional S-locus genes during evolution. The downstream signaling protein, ARC1, is also required for the self-incompatibility response in some Arabidopsis and Brassica species, and its gene is deleted in the A. thaliana genome. In this study, we attempted to reconstitute the SCR-SRK-ARC1 signaling pathway to restore self-incompatibility in A. thaliana using genes from A. halleri and B. napus, respectively. Several of the transgenic A. thaliana lines expressing the A. halleri SCR13-SRK13-ARC1 transgenes displayed self-incompatibility, while all the transgenic A. thaliana lines expressing the B. napus SCR1-SRK1-ARC1 transgenes failed to show any self-pollen rejection. Furthermore, our results showed that the intensity of the self-incompatibility response in transgenic A. thaliana plants was not associated with the expression levels of the transgenes. Thus, this suggests that there are differences between the Arabidopsis and Brassica self-incompatibility signaling pathways, which perhaps points to the existence of other factors downstream of B. napus SRK that are absent in Arabidopsis species.
Bentonite and Biochar Mitigate Pb Toxicity in Pisum sativum by Reducing Plant Oxidative Stress and Pb Translocation
Authors: Zulqurnain Haider Muhammad , Hussain Sabir , Muhammad Adnan Ramzani Pia , Iqbal Mutahar , Iqbal Muhammad , Shahzad Tanvir , Fatima Maryam , Ali Khan Shahbaz , Khan Imran , Shahid Muhammad , Ibrahim Muhammad , Tanzeem Ull Haq Hafiz Syed , Mahmood Faisal
Published: December 2019

Lead (Pb)-polluted soils pose a serious threat to human health, particularly by transmitting this heavy metal to the food chain via the crops grown on them. The application of novel amendments in Pb-polluted soils can significantly reduce this problem. In this research, we report the effects of various organic and inorganic amendments i.e., bentonite (BN), biochar (BR), lignin (LN), magnesium potassium phosphate cement (CM) and iron hydroxyl phosphate (FeHP), on the Pb bioavailability in Pb-polluted soil, upon Pb distribution in shoots, roots, grain, the translocation factor (TF) and the bioconcentration factor (BCF) of Pb in pea (Pisum sativum L.) grain. Furthermore, effects of the said amendments on the plant parameters, as well as grain biochemistry and nutritional quality, were also assessed. Lead pollution significantly elevated Pb concentrations in roots, shoots and grain, as well as the grain TF and BCF of Pb, while reducing the nutritional quality and biochemistry of grain, plant height, relative water content (RWC), chlorophyll contents (chl a and chl b) and the dry weight (DW) of shoot, root and grain. The lowest Pb distribution in shoots, roots and grain were found with BN, FeHP and CM, compared to our control. Likewise, the BN, FeHP and CM significantly lowered the TF and BCF values of Pb in the order FeHP > CM > BN. Similarly, the highest increase in plant height, shoot, root and grain DW, RWC, chl a and chl b contents, grain biochemistry and the micronutrient concentrations, were recorded with BR amendment. Biochar also reduced grain polyphenols as well as plant oxidative stress. Given that the BR and BN amendments gave the best results, we propose to explore their potential synergistic effect to reduce Pb toxicity by using them together in future research.
AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana
Authors: Wan Xia , Peng Lu , Xiong Jie , Li Xiaoyi , Wang Jianmei , Li Xufeng , Yang Yi
Published: December 2019

Because they are sessile organisms, plants need rapid and finely tuned signaling pathways to adapt to adverse environments, including salt stress. In this study, we identified a gene named Arabidopsis thaliana stress-induced BTB protein 1 (AtSIBP1), which encodes a nucleus protein with a BTB domain in its C-terminal side and is induced by salt and other stresses. The expression of the β-glucuronidase (GUS) gene driven by the AtSIBP1 promoter was found to be significantly induced in the presence of NaCl. The sibp1 mutant that lost AtSIBP1 function was found to be highly sensitive to salt stress and more vulnerable to salt stress than the wild type WT, while the overexpression of AtSIBP1 transgenic plants exhibited more tolerance to salt stress. According to the DAB staining, the sibp1 mutant accumulated more reactive oxygen species (ROS) than the WT and AtSIBP1 overexpression plants after salt stress. In addition, the expression levels of stress-induced marker genes in AtSIBP1 overexpression plants were markedly higher than those in the WT and sibp1 mutant plants. Therefore, our results demonstrate that AtSIBP1 was a positive regulator in salinity responses in Arabidopsis.
Mechanisms of Plant Tolerance to RNA Viruses Induced by Plant-Growth-Promoting Microorganisms
Authors: Maksimov Igor , Sorokan Antonina , Burkhanova Guzel , Veselova Svetlana , Alekseev Valentin , Shein Mikhail , Avalbaev Azamat , Dhaware Prashant , Mehetre Gajanan , Singh Bhim , Khairullin Ramil
Published: December 2019

Plant viruses are globally responsible for the significant crop losses of economically important plants. All common approaches are not able to eradicate viral infection. Many non-conventional strategies are currently used to control viral infection, but unfortunately, they are not always effective. Therefore, it is necessary to search for efficient and eco-friendly measures to prevent viral diseases. Since the genomic material of 90% higher plant viruses consists of single-stranded RNA, the best way to target the viral genome is to use ribonucleases (RNase), which can be effective against any viral disease of plants. Here, we show the importance of the search for endophytes with protease and RNase activity combined with the capacity to prime antiviral plant defense responses for their protection against viruses. This review discusses the possible mechanisms used to suppress a viral attack as well as the use of local endophytic bacteria for antiviral control in crops.
Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities
Authors: Jampafuang Yattra , Tongta Anan , Waiprib Yaowapha
Published: December 2019

α- and β-Chitosan nanoparticles were obtained from shrimp shell and squid pen chitosan with different set of deacetylation degree (%DD) and molecular weight (MW) combinations. After nanoparticle formation via ionic gelation with sodium tripolyphosphate (TPP), the % crystallinity index (%CI) of the α- and β-chitosan nanoparticles were reduced to approximately 33% and 43% of the initial %CI of the corresponding α- and β-chitosan raw samples, respectively. Both forms of chitosan and chitosan nanoparticles scavenged superoxide radicals in a dose-dependent manner. The %CI of α- and β-chitosan and chitosan nanoparticles was significantly negatively correlated with superoxide radical scavenging abilities over the range of concentration (0.5, 1, 2 and 3 mg/mL) studied. High %DD, and low MW β-chitosan exhibited the highest superoxide radical scavenging activity (p < 0.05). α- and β-Chitosan nanoparticles prepared from high %DD and low MW chitosan demonstrated the highest abilities to scavenge superoxide radicals at 2.0–3.0 mg/mL (p < 0.05), whereas α-chitosan nanoparticles, with the lowest %CI, and smallest particle size (p < 0.05), prepared from medium %DD, and medium MW chitosan showed the highest abilities to scavenge superoxide radicals at 0.5–1.0 mg/mL (p < 0.05). It could be concluded that α- and β-chitosan nanoparticles had superior superoxide radical scavenging abilities than raw chitosan samples.
Flexible Energy Storage System—An Introductory Review of Textile-Based Flexible Supercapacitors
Authors: Hui Chi-yuen , Kan Chi-wai , Mak Chee-leung , Chau Kam-hong
Published: December 2019

Recently, researchers have become interested in exploring applications of rechargeable battery storage technology in different disciplines, which can help our daily life, such as textile-based supercapacitors. This paper briefly describes this development and classification of supercapacitors. Besides, various types of materials which are commonly used to prepare supercapacitors, such as carbons, metal oxides, alkaline earth metal salts and polymers, are introduced. Moreover, applications and methodology to prepare textile materials with supercapacitors are described. Finally, the commonly used non-destructive measuring methods for textile-based supercapacitors are also introduced.
Measuring the Renewable Energy Efficiency at the European Union Level and Its Impact on CO2 Emissions
Authors: Busu Mihail
Published: December 2019

Low carbon emissions have a great importance in our life. The increasing importance of carbon emission levels have attracted the interests of researchers and academics in the field. In this article, a panel data econometric model is developed to measure the relationship between renewable energy, energy productivity, population, urbanization, motorization, and Gross Domestic Product (GDP) per capita and their impacts on carbon dioxide CO2 emissions. Data used in this study was collected from the European Statistical Office (EUROSTAT) and five statistical hypotheses were tested and validated through a multilinear regression model using the Econometric Views (Eviews) 10.0 statistical software. The Hausman test was used to choose between a model with fixed effects and a model with random effects, and the variance inflection factor (VIF) was used to test the collinearity between the independent variables. The author’s findings indicate that renewable energy at the European Union (EU) level has a positive impact on low-carbon emissions. It was found that a 1% increase in renewable energy consumption would reduce the CO2 emissions by 0.11 million tons, while population growth and urbanization degree add more restrictions to the econometric equation of the impact on carbon emissions.
Multivariate Analysis and Machine Learning for Ripeness Classification of Cape Gooseberry Fruits
Authors: De-la-Torre Miguel , Zatarain Omar , Avila-George Himer , Muñoz Mirna , Oblitas Jimy , Lozada Russel , Mejía Jezreel , Castro Wilson
Published: December 2019

This paper explores five multivariate techniques for information fusion on sorting the visual ripeness of Cape gooseberry fruits (principal component analysis, linear discriminant analysis, independent component analysis, eigenvector centrality feature selection, and multi-cluster feature selection.) These techniques are applied to the concatenated channels corresponding to red, green, and blue (RGB), hue, saturation, value (HSV), and lightness, red/green value, and blue/yellow value (L*a*b) color spaces (9 features in total). Machine learning techniques have been reported for sorting the Cape gooseberry fruits’ ripeness. Classifiers such as neural networks, support vector machines, and nearest neighbors discriminate on fruit samples using different color spaces. Despite the color spaces being equivalent up to a transformation, a few classifiers enable better performances due to differences in the pixel distribution of samples. Experimental results show that selection and combination of color channels allow classifiers to reach similar levels of accuracy; however, combination methods still require higher computational complexity. The highest level of accuracy was obtained using the seven-dimensional principal component analysis feature space.
Proactive Energy Optimization in Residential Buildings with Weather and Market Forecasts
Authors: Simmons Cody R. , Arment Joshua R. , Powell Kody M. , Hedengren John D.
Published: December 2019

This work explores the development of a home energy management system (HEMS) that uses weather and market forecasts to optimize the usage of home appliances and to manage battery usage and solar power production. A Moving Horizon Estimation (MHE) application is used to find the unknown home model parameters. These parameters are then updated in a Model Predictive Controller (MPC) which optimizes and balances competing comfort and economic objectives. Combining MHE and MPC applications alleviates model complexity commonly seen in HEMS by using a lumped parameter model that is adapted to fit a high-fidelity model. Heating, ventilation, and air conditioning (HVAC) on/off behaviors are simulated by using Mathematical Program with Complementarity Constraints (MPCCs) and solved in near real time with a non-linear solver. Removing HVAC on/off as a discrete variable and replacing it with an MPCC reduces solve time. The results of this work indicate that energy management optimization significantly decreases energy costs and balances energy usage more effectively throughout the day. A case study for Phoenix, Arizona shows an energy reduction of 21% and a cost reduction of 40%. This simulated home contributes less to the grid peak load and therefore improves grid stability and reduces the amplitude of load-following cycles for utilities. The case study combines renewable energy, energy storage, forecasts, cooling system, variable rate electricity plan and a multi-objective function allowing for a complete home energy optimization assessment. There remain several challenges, including improved forecast models, improved computational performance to allow the algorithms to run in real time, and mixed empirical/physics-based machine-learning methods to guide the model structure.
Theories of the Origin of the Samaritans—Then and Now
Authors: Kartveit Magnar
Published: December 2019

The article describes the different models for understanding the origin of the Samaritans: the Samaritans’ own view; Flavius Josephus’ two stories; a model based upon the results of the excavations of the cities of Samaria and Shechem, plus information from ancient authors; new insights from the Dead Sea Scrolls; and models based on the results of the Mount Gerizim excavations; and the Delos inscriptions. Each of these models has its modern followers in scholarship, and their various adherents are named. A last part of the article is devoted to the state of the question of the origin of the Samaritans. The presentation is organized according to the sources because the material at hand has produced different solutions to the pertinent questions. Through quoting the texts and presenting the results of the excavations, the author gives the reader an opportunity to form her or his own opinions, both on the different theories and on the origin of the Samaritans.
Quantifying the Congruence between Air and Land Surface Temperatures for Various Climatic and Elevation Zones of Western Himalaya
Authors: Singh Shaktiman , Bhardwaj Anshuman , Singh Atar , Sam Lydia , Shekhar Mayank , Martín-Torres F. Javier , Zorzano María-Paz
Published: December 2019

The surface and near-surface air temperature observations are primary data for glacio-hydro-climatological studies. The in situ air temperature (Ta) observations require intense logistic and financial investments, making it sparse and fragmented particularly in remote and extreme environments. The temperatures in Himalaya are controlled by a complex system driven by topography, seasons, and cryosphere which further makes it difficult to record or predict its spatial heterogeneity. In this regard, finding a way to fill the observational spatiotemporal gaps in data becomes more crucial. Here, we show the comparison of Ta recorded at 11 high altitude stations in Western Himalaya with their respective land surface temperatures (Ts) recorded by Moderate Resolution Imagining Spectroradiometer (MODIS) Aqua and Terra satellites in cloud-free conditions. We found remarkable seasonal and spatial trends in the Ta vs. Ts relationship: (i) Ts are strongly correlated with Ta (R2 = 0.77, root mean square difference (RMSD) = 5.9 °C, n = 11,101 at daily scale and R2 = 0.80, RMSD = 5.7 °C, n = 3552 at 8-day scale); (ii) in general, the RMSD is lower for the winter months in comparison to summer months for all the stations, (iii) the RMSD is directly proportional to the elevations; (iv) the RMSD is inversely proportional to the annual precipitation. Our results demonstrate the statistically strong and previously unreported Ta vs. Ts relationship and spatial and seasonal variations in its intensity at daily resolution for the Western Himalaya. We anticipate that our results will provide the scientists in Himalaya or similar data-deficient extreme environments with an option to use freely available remotely observed Ts products in their models to fill-up the spatiotemporal data gaps related to in situ monitoring at daily resolution. Substituting Ta by Ts as input in various geophysical models can even improve the model accuracy as using spatially continuous satellite derived Ts in place of discrete in situ Ta extrapolated to different elevations using a constant lapse rate can provide more realistic estimates.