bluuebunny/crossref_metadata_2025 huggingface dataset:
Rationality Evaluation of Packaging Materials and Study on Impurity Profiling of Cilnidipine Preparations by HPLC-Q-TOF/MS
Authors: Zeng Hongxia , Wang Jian , Zhu Bingqi , Shao Peng , Zhong Weihui
Published: May 2018

Background:

The rationality of cilnidipine tablets and capsules with four different pharmaceutical
packaging materials was evaluated, and the structure of photodegradation impurities was analyzed
by LC-Q-TOF.

Materials and Methods:

The impurity profiling of commercial cilnidipine tablets and capsules was
analyzed by LC-Q-TOF for the further improvement of official monograph in pharmacopoeias and the
source of the impurities was investigated. The contents of photodegradation impurities were analyzed
by HPLC, and remarkable difference in the formation of the photodegradation impurities in cilnidipine
tablets and capsules with four different pharmaceutical packaging materials was observed. The shading
effect of the four packaging materials was investigated by UV-Vis spectrophotometer and a remarkable
difference was detected, which might be responsible for the difference in the formation of photodegradation
products. The structures of photodegradation products were further characterized by LC-Q-TOF
MS/MS. Five impurities in commercial cilnidipine tablets and capsules were separated and identified
based on the high resolution MS/MS data.

Results:

The obtained results revealed that the impurity III was derived from the ethanol solution of
cilnidipine when it was exposed to light, and the impurity II was produced when the cilnidipine powder
was exposed to light directly. The concentrations for 50% reduction of impurity II and impurity III on
the Chinese hamster lung cells (CHL) with CellTiter-Glo method were studied and the results indicated
that the cytotoxicity of impurity II and impurity III on CHL cells was larger than cytotoxicity of cilnidipine.

Conclusion:

On the basis of our study, we suggested that the pharmaceutical packaging materials of
cilnidipine tablets should be modified.
Targeting Negative Regulators of TRIF-dependent TLR Signaling Pathway as a Novel Therapeutic Strategy
Authors: Mosaddeghi P. , Nezafat N. , Negahdaripour M. , Eslami M. , Ghasemi Y.
Published: May 2018

Background:

Toll-Like Receptors (TLRs) are a subclass of pathogen-associated molecular
patterns (PAMPs). There is a growing interest in the use of TLR agonists for various
pathological dysfunctions, including cancer, microbial infections, and inflammatory diseases.
TLR3/4 agonists that can induce TIR-domain-containing adapter-inducing interferon-β (TRIF)-
dependent pathway have shown fewer toxic immunostimulatory responses in comparison to other
small molecules. Furthermore, TLR3 agonists indicate promising anti-tumor potential in cancer
immunotherapy either as vaccine adjuvant or monotherapy.

Objective: It is logical to assume that the induction of the genes that are involved in TRIF pathway
to augment their pleiotropic effects on different cells via TLR agonists, could enhance the treatment
process of disease while minimizing the toxicity related to using other small molecules.

Methods:

An extensive literature search to identify the negative regulators of TRIF-dependent
signaling pathway and their biological functions was performed from two databases PubMed and
Scopus.

Results:

Negative regulators of TRIF signaling pathways were identified. In addition, structure
and function of sterile α- and armadillo-motif containing protein (SARM), the only TIR domaincontaining
adaptor protein that inhibits TRIF-dependent activation, were briefly reviewed.

Conclusion:

We proposed that the manipulation of TRIF signaling pathway via targeting its negative
regulators could be used as an approach to modulate the functions of this pathway without
undesired toxic proinflammatory responses.
Innovating From Within: A Process Model for User-Centered Digital Development in Academic Medical Centers (Preprint)
Authors: Chokshi Sara Kuppin , Mann Devin M.
Published: May 2018

BACKGROUND

Design thinking and human-centered design approaches have become increasingly common in health care literature, particularly in relation to health information technology (HIT), as a pathway toward the development of usable, diffusible tools and processes. There is a need in academic medical centers tasked with digital innovation for a comprehensive process model to guide development that incorporates current industry trends, including design thinking and lean and agile approaches to digital development.

OBJECTIVE

This study aims to describe the foundations and phases of our model for user-centered HIT development.

METHODS

Based on our experience, we established an integrated approach and rigorous process for HIT development that leverages design thinking and lean and agile strategies in a pragmatic way while preserving methodological integrity in support of academic research goals.

RESULTS

A four-phased pragmatic process model was developed for user-centered digital development in HIT.

CONCLUSIONS

The model for user-centered HIT development that we developed is the culmination of diverse innovation projects and represents a multiphased, high-fidelity process for making more creative, flexible, efficient, and effective tools. This model is a critical step in building a rigorous approach to HIT design that incorporates a multidisciplinary, pragmatic perspective combined with academic research practices and state-of-the-art approaches to digital product development to meet the unique needs of health care.
Educational Development in Africa: Prospects and Challenges of Teaching/Learning History in Nigeria
Authors: Afolabi Oluwaseun O.
Published: May 2018

The paper discusses the challenges facing history teaching in Nigeria. The scope of the study is limited to private/public schools in Oyo State, Nigeria. The study starts by tracing the problem from the advent of missionaries to Nigeria in which their purpose of establishing mission schools was not to teach African history but to evangelize and to produce middlemen who will act as junior officers. The findings show that history as a subject was in the curriculum of both junior and senior secondary school. However, it was removed from junior secondary school curriculum and remained only in senior secondary school curriculum. Though only few senior secondary schools in Oyo State teach history subject due to lack of adequate knowledge by students in learning African history. Also, due to the reviewed policy, history teachers and textbooks are inadequate. Thus, in this present dispensation in Nigeria, African history education is facing challenges in terms of teaching and learning, apparently on the verge of extinction. The paper concludes by stating the methods of teaching history, prospects and challenges attached to it.
Impact of Educational Programs That Include Elements of Positive Psychology for Developing the Emotional Competences of the Students
Authors: Dimovska Marija Kotevska
Published: May 2018

Modern education considers schools and faculties for institutions whose goals have a broader scope than just an academic competence. Educational institutions have an obligation to completely prepare young people for adulthood and improve their optimal functioning and well being. This paper shows how the application of the positive psychology in the education can help schools and faculties to develop and maintain optimal functioning of the students and the employees. Educational institutions should approach strategically in the creation of positive educational programs that include elements of positive psychology, which positively reflects on the well-being and positive educational climate. The empirical findings of this paper confirm the role of positive psychology in education for improving the subjective dimensions of students: happiness, optimism, self-confidence, social and emotional competence. Students who attended educational programs with elements of positive psychology, have shown higher personal, emotional, social achievements and general well-being, which are prerequisites for realizing their potential. These findings imply the need to expand the arsenal of education through a positive education that includes the development of positive emotions that promote happiness and well-being.
Evaluating Negative Environmental Impacts Caused by Dam Construction
Authors: Zare Rahim , Kalantari Behzad
Published: May 2018

Dams are one of the most important structures in the water resources transmission and storage systems. Thus, given the growth and development of dam construction in the world, evaluating environmental consequences of the dam has high importance. Dams leave positive and negative impacts on the environment. The destructive impacts of dam construction include social, economic, hydraulic, biological, health, and atmospheric impacts, etc., leading to change and complexity. Hence, given the importance of the positive impacts of dams, it is essential that negative environmental impacts of the dam to be minimized for sustainable development. The current article evaluates the negative environmental impacts caused by dam construction.
Fuentes y proyección de las causales de incapacidad
Authors: Laje Alejandro , Lanzavecchia Gabriel
Published: May 2018

Se analiza la evolución del concepto de las categorías jurídicas persona, capacidad e incapacidad con el fin de hacer una proyección de la evolución de las causales de incapacidad en el derecho, tomando como criterio la legislación de la República Argentina. Se describen las fuentes de estos institutos y su validez a la luz del principio general de solidaridad jurídica
Manajemen Risiko Berbasis Syariah
Authors: Trimulato Trimulato
Published: May 2018

This paper is descriptive qualitative, which describes risk management, and risk management in Islam, and risk management in shariah banking. The results of this paper indicate that risk management has a variety of processes, namely, the identification, evaluation and measurement, and management of risk. Risk management has been in line with Islam and different maysir. As well as risk management in Islamic banks have been set by the Indonesian bank in order to maintain the existence and improve the quality of shariah banking.
Ciclagem de nutrientes em ecossistemas de pastagens tropicais
Authors: Souza Marcondes , Jardim Alexandre Maniçoba da Rosa Ferraz , Araújo Júnior George do Nascimento , Silva José Raliuson Inácio , Leite Maurício Luiz de Mello Vieira , Teixeira Vicente Imbroisi , Silva Thieres George Freire da
Published: May 2018

The degradation of Brazilian pastures has been an increasing problem, especially in agricultural frontier regions, where the management practices adopted are inadequate, causing an imbalance in this ecosystem. Management practices that favor nutrient cycling appear in this scenario as an alternative for the recovery of degraded pastures. In the case of pastures, the largest reservoirs of nitrogen (N) and carbon (C) are the atmosphere, soil, plant species and grazing animals, being that the dynamics of these nutrients is controlled by biotic and abiotic factors. The litter and the animal excreta represent the main routes of return of the nutrients to the ecosystem through the decomposition by microorganisms. The use of legume species intercropped with pasture is a good strategy to increase soil nitrogen content, minimizing costs with nitrogen fertilization, in addition to improving the quality of the litter.
Aplicação das boas práticas agrícolas na produção de leite
Authors: Pereira Neta Izabel , Rodrigues da Silva Adriana , Do Carmo Santos Giselly Monique , Silva Athiê Thaynná , Silva Reis Wiviane Cristina , Costa Seixas Vitória Nazaré
Published: May 2018

The study aimed to assess the microbiological conditions and conservation, to the Good Agricultural Practices (GAP) in the production of milk. Hygiene during the process of obtaining and storage of milk in the dairy property can be determined by using the total bacteria count and Somatic cell count. The development of milk production chain followed by the desired quality for the product are challenges of rural producers, in order to guarantee the consumer a product safe, nutritious and healthy, free from microbiological contamination. With that, the implementation of the BPA's, from the moment of your arrival to the milking to dairy farm for your processing is important to preventing possible contamination during the process of obtaining of the final product. Therefore should follow standards for obtaining a good raw material.
A novel 4-sensor fast-response aerodynamic probe for non-isotropic turbulence measurement in turbomachinery flows
Authors: Chasoglou Alexandros C. , Mansour Michel , Kalfas Anestis I. , Abhari Reza S.
Published: May 2018

In modern computational studies for turbomachinery applications, time, length scales and isotropy of turbulent structures are important for representative modelling. To this end, experimental data are essential to validate the numerical tools. The current article presents the development and application of a newly designed 4-sensor Fast Response Aerodynamic Probe (FRAP-4S) enabling time-resolved measurement of the three-dimensional unsteady flow velocity vector in turbomachines. The miniature multi-sensor probe demonstrates a 4 mm probe-tip. In the first part of this article the design, manufacturing and calibration results of the FRAP-4S are presented in detail. To assess the newly developed probe accuracy, comparison against traditional instrumentation developed at the Laboratory for Energy Conversion is also provided. In the second part of this work, measurements are performed at the rotor exit of a one-and-a-half stage, unshrouded and highly-loaded axial turbine configuration. The results showed increased level of unsteadiness and turbulence levels with peak-to-peak fluctuation from 5 to 35%. More importantly, in some regions stream-wise unsteadiness was found to be ten times higher, compared to the cross-wise components, an indication of the high degree of anisotropy.
REGULATIONS OF REGISTRATION AND IMPORT OF MEDICAL DEVICE IN INDIA
Authors: Budhwaar Vikaas , Rohilla Yogesh , Choudhary Manjusha , Kumar Prateek
Published: May 2018

India is a huge market for medical devices and is increasing constantly for the last few years. The registration certificate and import license is mandatory for a manufacturer of India who wishes to import any medical device in India. If a company which wants to imports its medical devices in India does not have a registered office in India it needs Indian agent authorized by CDSCO, to do so. Duly filled form-10 is required to be submitted for import license, while form-44 duly filled is required to be submitted for marketing authorization of a new medical device or its re-registration. The review focuses on regulation concerned to the registration procedures import of a new medical device in India with latest amendments in the regulation concerned.
ETNOCÍDIO NA BANDA ORIENTAL: A RESISTÊNCIA ÉTNICA DOS CHARRUA PERANTE A COLONIZAÇÃO.
Authors: Moro Taciane , Colvero Ronaldo Bernadino
Published: May 2018

Os Charrua eram povos originários que viviam no território que atualmente delimita a fronteira oeste do Estado do Rio Grande do Sul e também territórios que hoje delimitam os países da Argentina e Uruguai. Esse grupo indígena tinha uma cultura e forma de viver própria, no período da colonização, a partir do Séc. XVI essa cultura sofreu algumas transformações, no entanto, essa etnia conseguiu prevalecer a sua essência mantendo alguns padrões culturais distintos dos colonizadores, o que gerou o etnocídio e quase extermínio dos mesmos. Sendo assim, a presente pesquisa tem por objetivo analisar como os povos originários Charrua enfrentaram a colonização, como os mesmos conseguiram manter o seu modo de vida indígena absorvendo algumas novas práticas e principalmente, quais foram as razões que levaram o etnocídio dos charrua nas batalhas de Salsipuedes e Mataojos (1830-1834). Esse trabalho foi desenvolvido através da metodologia qualitativa, através da pesquisa bibliográfica em diferentes fontes como livros, artigos e dissertações
Gênero e sexualidade: diálogos e inquietações com/da/na educação básica
Authors: Gamalho Nola Patrícia , Epitácio Sara , Rosa Gladis Kunz , Da Silva Ferreira Ewerton
Published: May 2018

O presente artigo tem por objetivo problematizar as discussões sobre currículo, gênero e sexualidades. Parte-se das atividades do projeto de extensão: "Construções e desconstruções do gênero e sexualidade no currículo: tecendo perspectivas para uma educação multicultural", realizado pela Universidade Federal do Pampa - (UNIPAMPA), campus São Borja, juntamente com as escolas da rede de Educação Básica do município. O desenvolvimento dessas atividades, ocorridas no formato de palestras, rodas de debate e atividades práticas, foram fundamentais para propiciar espaços de diálogo. No município em questão predominam historicamente latifúndios voltados para a pecuária e monoculturas, o que implicou em uma estrutura social fortemente assentada no patriarcado e, desta forma, com ramificações nas organizações sociais, políticas, imaginários e representacionais. Portanto, o objetivo de dialogar e desconstruir imaginários de gênero e sexualidade, além de ser um desafio, é uma necessidade emergente. Assim, o texto discorre sobre o contexto espacial e histórico do município; desenvolve uma discussão conceitual sobre gênero, sexualidade e currículo.  Por fim, a partir da análise das atividades desenvolvidas, são tecidas reflexões sobre a identidade docente e práticas pedagógicas. Gênero, sexualidade e educação são temas intrincados e em disputa. Assim, as reflexões aqui expostas inserem-se na luta política e social para a constituição de sociedades que aprendam com as diferenças e diversidades.
Herramienta TIC para la docencia que facilita el aprendizaje de conceptos y la generación de competencias favorables al emprendimiento y la innovación: el caso ProfePlus
Authors: Guerra Portocarrero Ronnie
Published: May 2018

Este trabajo muestra cómo el uso de una TIC educativa, ProfePlus, posibilita que desde el aula se facilite en los estudiantes el aprendizaje de conceptos y la generación de competencias importantes. La metodología de ProfePlus se basa en la instrucción entre pares y el impacto de esta propuesta fue analizada en todas las competencias genéricas estipuladas por la Pontificia Universidad Católica del Perú (PUCP) para sus egresados: trabajo en equipo, participación en proyectos, aprendizaje autónomo, ética y ciudadanía, investigación, entre otros. Finalmente se muestran perspectivas futuras para ampliar ProfePlus y formar un ecosistema de innovación gracias a esta iniciativa. Este proyecto fue financiado por la PUCP a través de la Dirección Académica del Profesorado (DAP), apoyada por la Red Peruana de Universidades de Perú (RPU) e implementada por profesionales del Grupo de Investigación del Trabajo en Equipo y Emprendimiento (GITEE), y la Red de Emprendedores E-QUIPU.
La fenomenología de la imagen poética de Gaston Bachelard
Authors: Puelles Romero Luis
Published: May 2018

RESUMENDespués de los intentos de aplicación del psicoanálisis tanto al ámbito de la racionalidad científica como a las obras literarias llevados a cabo por Bachelard entre filanes de los años treinta y la década de los cuarenta, la aparición en 1957 de la peotique de l'espace inaugura un periodo de alejamiento del método psicoanalítico y de afilicación a la escuela fenomenológica. En las páginas que siguen se ofrecen algunas de las claves de esta última época, la cual supone una fecunda contribución al análisis de la imagen poética. PALABRAS CLAVEBACHELARD-ESTÉTICA-FENOMENOLOGÍA-POSEÍAABSTRACTAfter the attempts to apply psycoannalysis both to scientific rationality and to literaly works made by bachelard betwerrn the end of the thirties and the forties, the publicación in 1957 of his la poethique e l'espace inaugurates a period of retrear from the psycoanalytic method and from the phenomenological school. This paper presents some of the clucs of this latest period, characterezied by a fertile contribution to the anlaysis of the poetic imageKEYWORDSBACHELARD-AESTHETICS-PHENOMENOLOGY-POETRY
Development of Research and Innovation Into the Diversity of Bioresources. Food Security Perspectives
Authors: Stan Simona Nicoleta , Strateanu Amalia Gianina
Published: May 2018

Doctrine, legislation and jurisprudence have importance in defining typologies of biodiversity bioresources. Matters of doctrine mainly concern to philosophy of life and the complexity of practical and theoretical hypostases in the current global geopolitical order. In a globalized world, with a society strained by social disorder, among the risks humanity, food supply has become a source of insecurity, in addition to the decline of the natural resources, leading to an intensification of problems at local, zonal, regional and/or global level. Multidimensionality of the concept of security includes demographic and food security, energy and the environment, not only military and political-military level. All these components also guarantee the sovereignty of any state. Food security is a complex and general problem of humanity that all countries are responsible for. FAO defines food security as "access of all people directly to the food they need" to meet their vital functions and lead a healthy and active life ". The responsibility of the present generation is to ensure the right to food for future population, through an efficient, fair and rational management of the Earth bioresources. They belong to the common heritage of mankind and to face the challenges of the safety and food security ahead, on long-term we need a bioethic, systematic and multidisciplinary approach for the right to adequate available and accessible food.
The Evaluation of the Bean Autochthonous Germoplasma in Korça Region
Authors: Maho Adrian , Mero Gjergji
Published: May 2018

Bean is one of the main legacy crops that play an important role in the planting structure of Korça region. Appreciating the bean as a rich food for man, the ancients have particularly been careful in the past to select and preserve the cultivars that have adapted to the ecological conditions of the country. This is evidenced by the large number of legumes planted or labeled according to the areas or villages where they have spread more. At present, this property poses a potential risk, the phenomenon of "genetic erosion". Farmers in the region use the zoned populations of beans. The study of these populations constitutes a necessity for the preservation of the autochthonous germoplasma of the beans as well as the competitive aspects and their spreading. The study revealed the germoplasma of autochthonous bean populations for the region of Korca.
Medication Errors Caused by Nurses and Physicians in a Swiss Acute Care Community Hospital: Frequency and Correlation to Nurses’ Reported Workload / Von Pflegefachpersonen und Ärzten/-innen verursachte Medikamentenfehler in einem Schweizer Akutspital: Häufigkeit und Korrelation zur Arbeitsbelastung von Pflegefachpersonen
Authors: Rapphold Benjamin David , Metzenthin Petra , Oertle Marc , Küng Kaspar
Published: May 2018

Objectives

This study was carried out in a Swiss acute care community hospital to investigate the frequency, type, causes and potential clinical consequences of medication errors (MEs) caused by nurses and physicians in all stages of a technology-supported medication process, the relationship between the nurses’ workload and the medication administration errors (MAEs) and their reason for workload.

Methods

In this descriptive cross-sectional study, a questionnaire, the adapted Medication Error Self Reporting Tool (A-MESRT), was used to identify MEs in all stages of the medication process and record nurses’ self-perceived workload during medication administration.

Results

A total of 1936 completed A-MESRTs were returned. A total of 751 (38.8%) respondents reported different MEs. The highest number of errors occurred during medication administration (43%), followed by errors during dispensing (34%) and physician ordering errors using a computerised physician order entry (CPOE) system (23%). Of the 768 (100%) handwritten orders, 232 (30.2%) were erroneous. Moreover, the greater the individual nurse’s workload during a shift, the higher was the relative probability of committing an MAE (χ2 = 85.479, df = 1, OR = 2.129, p < 0.001). Furthermore, the three main causes of high or very high workload were revealed: (1) many newly operated patients to monitor; (2) complex multimorbid patients, for example, those with delirium; and (3) patients with complications after surgery.

Conclusion

The A-MESRT showed that the highest rate of MEs caused by nurses and physicians is in the non-technologically supported steps, demonstrating the potential benefits of a technology-supported medication process. Moreover, this study revealed a statistically significant correlation between nurses’ workload and MAEs.
Screening by Social Workers in Medical Patients with Risk of Post-Acute Care Needs: A Stepped Wedge Cluster Randomized Trial / Evaluation eines Screenings durch Sozialarbeiter bei medizinischen Patienten mit einem Risiko für post-akuten Nachsorgebedarf: eine stepped wedge clusterrandomisierte Studie
Authors: Conca Antoinette , Ebrahim Doaa , Noack Sandra , Gabele Angela , Weber Helen , Prins Mehrnaz , Keller Anja , Hari Mariann , Engel Angela , Regez Katharina , Schild Ursula , Schuetz Philipp , Müller Beat , Haubitz Sebastian , Kutz Alexander , Huber Andreas , Faessler Lukas , Schäfer-Keller Petra
Published: May 2018

Background

Elderly patients often need post-acute care after hospital discharge. Involvement of social workers can positively affect the discharge planning process.

Aim

To investigate the effect of screening patients at risk for post-acute care needs by social workers on time with respect to social workers’ notification, length of stay and delays in discharge compared to usual care.

Methods

Cluster randomized stepped wedge trial design for five clusters (wards) and two steps (control to intervention) was used. A total of 400 patients (200 per period) with high risk of post-acute care needs (defined as Post-Acute Care Discharge score, PACD ≥ 7) were included. Social workers performed a screening to decide about self-referral to their services (intervention period), which was compared to a highly structured standard SW notification by physicians and nurses (control period). A Generalized Estimating Equations model adjusted the clustering and baseline differences.

Results

A total of 139 patients were referred to social services (intervention: n = 76; control: n = 63). Time to social workers’ notification was significantly shorter in the intervention period when adjusted for all the differences in baseline (Mdn 1.2 vs 1.7, Beta = -0.73, 95%-CI 1.39 to -0.09). Both the length of stay and the delayed discharge time in nights showed no significant differences (Mdn 10.0 vs 9.1, Beta = -0.12, 95%-CI 0.46 to .22 nights 95%-CI, resp. Mdn 0.0 vs 0.0, Beta = .11, 95%-CI -0.64 to 0.86).

Conclusion

Screening speeded up social workers’ notification but did not accelerate the discharge processes. The screening by social workers might show process improvement in settings with less structured discharge planning.
“I feel I am a human being again” Care and Case Management supporting the self-reliance of older people with multimorbidity living alone: Findings of an interpretive-hermeneutical analysis / „Ich bin wieder ein Mensch geworden” Care und Case Management als Unterstützung der Eigenständigkeit allein lebender, mehrfacherkrankter, älterer Menschen: Ergebnisse einer interpretativ-hermeneutischen Analyse
Authors: Schmidt Stefan , Luderer Christiane
Published: May 2018

Objective

This interpretive-hermeneutical study examined how the possibilities offered by Care and Case Management (CCM) were perceived by older people with multimorbidity living alone with regard to their remaining self-reliant and independent.

Method

Guided interviews were conducted with 20 CCM users (60% women, age range between 64 and 89 years). The sampling and evaluation processes were based on the Grounded Theory method.

Results

Before CCM was initiated, the interviewed persons had been confronted with a multitude of offers and services for care provided by various organizations, which had made it difficult for them to get an overall view. Through CCM they felt strengthened in their everyday life because each individual life situation became the focal point of the care they needed and because they gained access to appropriate assistance, which would not have been possible without CCM.

Conclusions

CCM offers older people content-related and organizational support in various areas of life, thus enabling them to have a self-determined way of life in their own homes.
Effects of miR-99a on the migration and proliferation of glioma cells
Authors: Xu Yifan , Lu Tianyu , Xu Wu , Dai Yuxiang , Liang Weibang , Jin Wei
Published: May 2018

Background To evaluate the effects of miR-99a on the migration and proliferation of glioma cells. Materials and Methods: Glioma cell line LN229 with stable up-regulation of miR-99a was constructed by transfection of hsa-miR-99a mimics, and cells with stable miR-99a knock-down were established by transfection of hsa-miR-99a inhibitor. The proliferation capacities of two groups were detected by the MTT assay, and their migration capacities were detected by the scratch assay. Results: LN229 cells with stable up-regulation and knock-down of miR-99a were successfully constructed. Up-regulating miR-99a inhibited the proliferation and migration of glioma cells, but knocking down this gene promoted their proliferation and migration. Conclusion: MiR-99a significantly affected the proliferation and migration of glioma cells, as a potentially eligible target for glioma therapy.
Antibiotic susceptibility and resistance profiles of Romanian Clostridioides difficile isolates
Authors: Macovei Ioana , Lemeni Daniela , Șerban Roxana , Niculcea Andreea , Popescu Gabriel A. , Nica Maria , Petrini Anca , Mihăescu Grigore
Published: May 2018

This study investigated the antibiotic susceptibility patterns and genetic resistance markers of 35 C. difficile strains isolated from patients with C. difficile infection. Vancomycin, metronidazole, tigecycline, teicoplanin, rifampicin, moxifloxacin, cefotaxime, tetracycline, erythromycin, clindamycin, chloramphenicol, linezolid and imipenem MICs were determined for toxigenic strains belonging to PCR ribotypes (PR) 012 (2), 014 (4), 017 (3), 018 (2), 027 (17), 046 (2), 087 (3) and 115 (2). Results showed vancomycin, metronidazole, tigecycline and teicoplanin to be active against all isolates. High resistance rates were noticed against cefotaxime (n = 35), clindamycin (n = 33), imipenem (n = 31), moxifloxacin (n = 25), erythromycin (n = 25) and rifampicin (n = 22). Linezolid-resistance was found in three isolates (PR 017/2, PR 012/1), showing complex resistance (7-9 antibiotics). PR 012, 017, 018, 027 and 046 isolates (n = 26) were resistant to 5-9 antibiotics. Twelve resistance profiles (2-9 antibiotics) were detected. Rifampicin-moxifloxacin-cefotaxime-erythromycin-clindamycin-imipenem-resistance was predominant, being expressed by 18 strains (PR 027/17, PR 018/1). PCR results suggested tetracycline-resistance to be induced by the gene tetM. Three tetM-positive isolates (PRs 012, 046), were also tndX-positive, suggesting the presence of a Tn5397-like element. Only two MLSB-resistant strains (PR 012) had the ermB gene and chloramphenicol-resistance determinant catD was not detected, leaving room for further investigating resistance mechanisms. Multidrug resistance could be attributed to most analysed strains, underlining, once more, the impact of wide-spectrum antimicrobial over prescription, still a tendency in our country, on transmission of antimicrobial resistance and emergence of epidemic C. difficile strains generating outbreaks.
Sanger sequencing of MMR genes in a one-plate system
Authors: Negura Lucian , Negura Anca
Published: May 2018

Both incidence and mortality of colorectal cancer (CRC) in Romania have shown a continuous increase during the last decades. Hereditary Non-Polyposic Colorectal Cancer (HNPCC), also known as Lynch syndrome, is mainly attributable to mismatch repair (MMR) genes MSH2, MSH6, and MLH1. Individuals carrying germ-line mutations of these genes present high lifetime risk of colorectal and other cancers, compared to non-carriers. Oncogenetics is developed worldwide nowadays, for identifying hereditary predisposition to cancer and offering appropriate clinical follow-up to patients and mutation carriers in Lynch families. Molecular oncogenetic diagnosis in Lynch syndrome is based on complete Sanger sequencing of entire MMR genes, which is time and resources consuming, therefore needing an appropriate and adapted optimization. Conventional sequencing requires a sufficient number of available samples to be processed simultaneously, which increases the waiting time for diagnostic results. Complete analysis for only one patient meets difficult technical problems due to the complex co-amplification of all gene regions of interest within the same conditions, therefore increasing the costs and reducing the cost-effectiveness of the test. Here we present an original and robust technical protocol for sequencing the entire MSH2, MSH6, and MLH1 coding sequence for one patient in a single PCR plate. Our optimized and verified system overcomes all technical problems and offers a quick, robust, and cost-effective possibility to personalize molecular oncogenetic diagnosis in Lynch syndrome.
Antiphosphatidylethanolamine Antibodies and Deep Vein Thrombosis in Lupus Patients with Antiphospholipid Syndrome
Authors: Caraiola Simona , Jurcuț Ciprian , Dima Alina , Băicuș Cristian , Băicuș Anda
Published: May 2018

Backgroud. Which factors determine venous thrombotic events in some antiphospholipid syndrome (APS) patients and arterial thrombosis or conditions related to pregnancy in others has not been established yet. Purpose. The aim of this study was to search the antiphospholipid antibodies (APLAs) correlates in regard to deep vein thrombosis (DVT) in patients with systemic lupus erythematosus (SLE) and APS. Methods. Twenty-nine patients fulfilling the criteria of both SLE and APS were included. Complete anamnesis and clinical examination was performed on inclusion. Also, for all patients, disease activity was assessed by the SLEDAI score. An extended APLAs profile, ten Abs, was searched. Results. The titers of IgG anticardiolipin (aCL), IgG anti-β2 glycoprotein I (aβ2GPI), IgG antiphosphatidylethanolamine (aPE), and also of IgG antiprothrombin (aPT) were significant higher in patients with DVT history. After analysis by ROC curve and univariate logistic regression, the strongest association was found for IgG aPE. Also, in multivariate analysis, SLEDAI score correlated with the DVT antecedents. Conclusions. IgG aPE might be involved in DVT pathogenic pathways in patients with SLE and APS as their titers remain significantly higher in patients with previous DVT. Lupus patients with DVT events represent a subgroup of patients with more severe underlying pathology.
In Vitro Antimicrobial Activities of 6-Substituted-3(2H)- pyridazinone-2-acetyl-2- (substituted/nonsubstitutedbenzal/ acetophenone) Hydrazone Derivatives
Authors: Abiha Gul Bayram , Bahar Leyla , Utku Semra
Published: May 2018

Aim: In vitro antibacterial activity of 6-substituted-3(2H)-pyridazinone-2-acetyl-2-(substituted/nonsubstitutedbenzal/ acetophenone) hydrazone derivatives were tested in common species causing hospital-acquired infections. Material and Method: Antimicrobial activities of the compounds were performed by determining minimum inhibitory concentration (MIC) value against four Gram-positive, five Gram-negative and four Candida species fungi. Modified serial microdilution method was carried out. Reference strains of American Type Culture Collection (ATCC) were used. Results: In general, eleven compounds exhibited considerable activity. Comparatively, compound 3 exhibited strong activity against Enterobacter hormaechei and 5, 11 were the most active against Acinetobacter baumannii at 31.25 μg/mL. Compounds 1,2,3,4,8 and 10 were found to be as active as positive control ampicillin trihidrate against Stenotrophomonas maltophilia. On the other hand, compounds 1,2,3,4,7,8,9,10 and 11 showed strong antifungal activitiy as much as fluconazole against Candida tropicalis. Compound 1 was mostly active against Candida albicans, Candida glabrata, Candida parapsilosis and Candida tropicalis. It was also revealed that the antifungal activity of compounds 1, 6, 7, 8 and 9 were higher than the others. Compound 1 and 8 exhibited the best activity against Candida glabrata and Candida parapsilosis respectively. Conclusions: All tested compounds showed better activity against Gram-negative bacteria and yeast than Gram-positive bacteria. These compounds may be considered as alternative antimicrobial agents in the treatment of multiple drug resistant Gram-negative, Gram-positive bacteria and fungal pathogens. Especially, we suggested that Compound 1 and 8 might be a promising candidate of new antifungal agents
Development of Numerical Model and Comparison with the Analytical Model for Predicting the Wire Effects in Wire Electric Discharge Grinding
Authors: Madhavadev Parthiban , Vijayan Krishnaraj , Mouleeswaran Senthilkumar
Published: May 2018

This paper discusses the development of a numerical model and its comparison with the analytical model to predict the wire deflection in WEDG. The movement of wire between upper and lower guides creates vibration during the machining process which can create micro deflection of the wire. The Finite Element Analysis model [FEA] for the wire deflection is analyzed and compared with the analytical model. The deflection of the wire due to the input current may affect the machining of micro parts, which will be used for further research in micro machining and also will help us to choose the parameters such as current, spark gap, wire feed, and the speed of the workpiece during micro machining with WEDG. The FEA results for various forces were compared with the analytical results and found that the developed numerical model results are in good agreement.
Fem Simulation of Thermal Cycles During Laser Welding of Aluminium
Authors: Sejč Pavol , Világoš Tomáš
Published: May 2018

The submitted paper deals with the simulation of thermal cycles during laser welding of aluminum sheets using Finite Element Method (FEM). After the design and creation of the simulation model, the simulation results were verified by the thermal cycles measured during the experiment on real samples. Verification has shown that, despite the differences that have resulted from some simplifications, the proposed model corresponds to the actual behavior of the material used during the laser welding process.
Design Thinking In Pharmacy Education: Inspiring Creative Problem Solving in the Next Generation of Pharmacists
Authors: Pugliese Robert Salvatore , Girone Gianna
Published: May 2018

Born from the world of product and service innovation, design thinking is gaining popularity as a method for introducing creative problem solving into the education of health professionals. Mindsets developed through practicing design thinking can help learners and educators address complex healthcare issues in a whole new way. This article aims to introduce the concepts of design thinking to the pharmacy educator, give examples of its use in pharmacy education, and discuss the value of including it in pharmacy education from both an educator and a student’s perspective.

 

Topic: Commentary
Suomen tieteellisen kirjastoseuran vuosi 2017 – strategiatyötä, seminaareja ja vaikuttamista
Authors: Saarti Jarmo , Nykyri Susanna
Published: May 2018

Viime vuoden keskeisenä tavoitteena oli STKS:n ensimmäisen strategian laadinta ja sen hyväksyminen kevätkokouksessa. Seuran historiateoksen toimitustyö eteni myös hyvin. Seura juhlisti Suomen itsenäisyyden 100-vuotisjuhlia järjestämällä onnistuneen juhlaseminaarin loppuvuodesta.Seura toimi myös aktiivisesti vaikuttamalla lainsäädännössä tapahtuvaan uudistustyöhön erityisesti tieteellisille kirjastoille tärkeissä asioissa. Myös koulutustoiminta oli vilkasta.
Kirjasto merkitsee jatkuvuutta, vapautta ja rauhaa
Authors: Lahikainen Johanna
Published: May 2018

Hyvä lukija, luet Signumin Suomi 100 -juhlanumeroa. Lehti perustuu tällä kertaa Suomen tieteellisen kirjastoseuran Suomi 100 -juhlaseminaarissaTieto, avoimuus ja kirjastot – jatkuuko menestystarina? pidettyihin puheenvuoroihin 23.11.2017 Tieteiden talolla. Lisäksi lukupaketistalöytyy journalistisen kirjoittamisen dosentin Maria Lassila-Merisalon ajankohtainen artikkeli, joka pohjautuu hänen pitämäänsäkeynote-puheenvuoroon Informaatiolukutaito-työryhmän seminaarissaTiedon monet kasvot – mikä on oikeaa tietoa, kuka valitsee faktat.
PEMETAAN LAJU DOSIS RADIASI MENGGUNAKAN TLD-100 DI PABRIK SEMEN
Authors: Abbas Alwis
Published: May 2018

Telah dilakukan pemetaan laju dosis radiasi di pabrik semen dengan sumber radiasi buatan (Sinar-X) dan alam. Pemetaan laju dosis radiasi masing-masing dilakukan di tiga tempat, yaitu pada radiasi buatan (Sinar-X) pemetaan dilakukan di ruangan CCR, On Stream 1 dan On Stream 2 sedangkan pada sumber radiasi alam pemetaan dilakukan di daerah storage, cement mill dan silo cement. Pemetaan dilakukan dengan cara memasang TLD-100 pada masing-masing ruangan dari 2 hingga 5 titik pemasangan, radiasi buatan (Sinar-X) pada ruangan CCR dilakukan pemasangan selama 17 hari dan pada ruangan On Stream dilakukan pemasangan selama 19 hari sedangkan pada radiasi alam dilakukan pemasangan selama satu bulan. Laju dosis radiasi buatan (Sinar-X) terbesar pada masing-masing ruangan terletak pada ruangan CCR di belakang pesawat Sinar-X sebesar 0,167 mSv/17 hari dan ruangan on stream 1 di samping pesawat Sinar-X sebesar 0,101 mSv/19 hari. Laju dosis radiasi alam terbesar pada masing-masing daerah terletak pada storage pasir besi sebesar 0,117 mSv/bulan, daerah cement mill 3 sebesar 0,141 mSv/bulan dan silo cement 2 sebesar 0,109 mSv/bulan. Menurut Badan Pengawas Tenaga Nuklir (BAPETEN) tingkat radiasi buatan (Sinar-X) maupun alam masih dikategorikan daerah pengawasan dengan tingkat radiasi sangat rendah.
Optimalisasi Persentase Serat Bambu Terhadap Sifat Fisis Papan Komposit Beton Ringan
Authors: Mahyudin Alimin , Yunita Devi , Milvita Dian
Published: May 2018

Telah dilakukan penelitian dengan judul optimalisasi serat bambu terhadap sifat fisis papan komposit beton ringan. Penelitian ini bertujuan untuk mengetahui pengaruh serat bambu terhadap sifat fisis papan komposit yang diharapkan dapat menghasilkan papan komposit beton ringan lebih kuat, lentur, ringanÂ  dengan persentase serat bambu yang optimum. Penelitian dilakukan dengan menggunakan metode two dimensional reinforcement (planar) dalam penyusunan serat bambu. Persentase serat yang digunakan pada masing-masing sampel, yaitu 0%; 0,6%; 1,2%;2,5% dan 5% serta foam agent 0,06%, dan tebal sampel papan 1 cm.Â  Berdasarkan hasil pengujian nilai densitas optimum diperolehÂ  sebesar 1,63 g/cm3 pada serat 0,6% dari volume sampel,Â  kuat lentur optimum terdapat pada serat 0,6% dari volume sampel bernilai 44,25 kg/cm2 sedangkan nilai kuat tekan optimum diperoleh sebesar 6,9 kg/cm2 pada serat 2,5% dari volume sampel. Nilai kuat lentur Â telah memenuhi standarÂ  SNI 03-2104Kata kunci: Papan komposit, serat bambu, foam agent.
Analisis Pengaruh Macrobending Serat Optik Pada Sensor Glukosa Dengan Metode Evanescent
Authors: Fridayanti Nola , Muldarisnur Muldarisnur , Harmadi Harmadi
Published: May 2018

Telah dilakukan analisis pengaruh macrobending serat optik yang telah dikupas claddingnya sepanjang 1 cm Â pada sensor glukosa dengan memanfaatkan interaksi gelombang evanescent pada serat optik dengan molekul glukosa. Cahaya dari laser dioda dengan panjang gelombang 650 nm ditransmisikan pada serat optik multimode. Pengupasan sebagian cladding serat mengakibatkan pelemahan intensitas terukur pada detektor akibat interaksi gelombang evanescent dengan molekul glukosa dalam urine. Penurunan intensitas cahaya terukur sebagai perubahan tegangan pada fotodioda yang mengindikasikan kosentrasi glukosa. Perbedaan konsentrasi glukosa berkaitan dengan indeks bias larutan glukosa (n2) yang menentukan kuat interaksi dan pelemahan gelombang evanescent. Intensitas cahaya yang sampai ke detektor meluruh secara eksponensial sebagai fungsi dari panjang pengupasan cladding serat optik. Hasil pengukuran menunjukkan bahwa tegangan keluaran dari sensor berkurang secara linear terhadap konsentrasi glukosa dengan sensitivitas yang diperoleh adalah 0,32558 (mg/dl)/mV, 0,34193 (mg/dL)/mV, dan 0,33677 (mg/dL)/mV.Â Kata kunci: macrobending, gelombang evanescent, serat optik, glukosa.
PENGARUH PENAMBAHAN GULA JAGUNG TERHADAP SIFAT MEKANIK DAN BIODEGRADABILITAS PLASTIK CAMPURAN POLYPROPYLENE BEKAS DAN PATI SAGU
Authors: Mulyadi Sri
Published: May 2018

Penelitian ini dilakukan untuk menentukan pengaruh gula jagung terhadap sifat mekanik dan biodegradabilitas plastik campuran polypropylene bekas dan pati sagu. Pada penelitian ini dibuat 5 sampel dengan variasi massa gula jagung yang berbeda. Pengukuran dilakukan terhadap kuat tekan dan kuat lentur dari setiap sampel. Penentuntuan tingkat degradabilitas ditentukan dengan mengubur sampel selama tujuh hari. Hasil menunjukkan bahwa kuat lentur dan kuat tekan plastik mengalami kenaikan dengan penambahan gula jagung. Nilai kuat tekan dan kuat lentur maksimum terdapat pada komposisi 90 gr : 10 gr : 10 gr yaitu 87,04 kg/cm2 dan 96,9 kg/cm2 . Ditinjau dari segi fisis setelah proses penguburan, plastik campuran dengan komposisi gula jagung terbanyak memiliki permukaan paling kasar dan paling banyak lobang. Hal ini dapat diartikan bahwa plastik dengan komposisi gula jagung terbanyak memiliki tingkat degradabilitas tertinggi.
PENGARUH PENAMBAHAN BKT PADA PIEZOELEKTRIK RAMAH LINGKUNGAN BI0,5NA0,5TIO3-BATIO3-BI0,5K0,5TIO3 (BNT-BT-BKT)
Authors: Mahyudin Alimin
Published: May 2018

Proses sintesis BNT-BT-BKT telah dilakukan dengan menggunakan metode solid state reaction. Sintesis BNT-BT dengan penambahan % mol BKT yaitu 2%, 5%, 8%, 15% juga mendapatkan struktur perovskite dengan komposisi yang optimal pada penambahan 5% mol BKT. Pola difraksi XRD pada bahan BNT-BT dengan penambahan % mol BKT menunjukkan adanya perubahan puncak-puncak yang tumpang tindih dari (200) rhombohedral menjadi (200) dan (002) dari tetragonal. Daerah perubahan fasa ini dikenal dengan MPB yang terjadi pada komposisi penambahan BKT 2%. Penambahan bahan dopan BKT ke BNT-BT menunjukkan perubahan pola difraksi sinar-x dengan pergeseran sudut 45Â° - 47Â°. Mikrograph SEM, menunjukkan penambahan 2% BKT pada BNT-BT memiliki bentuk partikel seperti jarum dengan panjang rata-rata 1,608 â€“ 2,589 Î¼m.
PEMBUATAN SEL FOTOVOLTAIK PASANGAN CuO/Cu DAN CuO/STAINLESS STEEL DALAM BENTUK TUNGGAL DAN SERABUT MELALUI METODA PERENDAMAN DENGAN NaOH
Authors: Tetra Olly Norita
Published: May 2018

The research about Photovoltaic cell of CuO/Cu and CuO/Stainless steel in single and fiber made by NaOH submersion method by using KCl electrolyte has been done. In this research, the KCl solution is used with different concentrations. The measurement of intensity and voltage is done before and after the Photovoltage cell is radiated by the sun light for approximately 5 minutes. The optimum concentration obtained is 0,3 M. The highest value for intensity and voltage is obtained on fiber CuO/Cu electrodes with the values are 0,364 mA and 0,170 V, measured at 11.00 â€“ 12.00 in the noon. The length time of submersion of Cu electrode with NaOH to is affecting the intensity and voltage of CuO production, submerging in relatively optimal in 24 hour. The Photovoltaic cell can be used with or without the sun light, with the voltage being produced is 0,109 mA for fiber CuO/Cu electrodes. CuO/Cu electrodes and Stainless steel CuO/Cu electrodes can be used on Photovoltaic cell.