bluuebunny/crossref_metadata_2025 huggingface dataset:
Statistical Modeling of the Early-Stage Impact of a New Traffic Policy in Milan, Italy
Authors: Maranzano Paolo , Fassò Alessandro , Pelagatti Matteo , Mudelsee Manfred
Published: February 2020

Most urban areas of the Po basin in the North of Italy are persistently affected by poor air quality and difficulty in disposing of airborne pollutants. In this context, the municipality of Milan started a multi-year progressive policy based on an extended limited traffic zone (Area B). Starting on 25 February 2019, the first phase partially restricted the circulation of some classes of highly polluting vehicles on the territory, in particular, Euro 0 petrol vehicles and Euro 0 to 3 diesel vehicles, excluding public transport. This is the early-stage of a long term policy that will restrict access to an increasing number of vehicles. The goal of this paper is to evaluate the early-stage impact of this policy on two specific vehicle-generated pollutants: total nitrogen oxides (NO x ) and nitrogen dioxide (NO 2 ), which are gathered by Lombardy Regional Agency for Environmental Protection (ARPA Lombardia). We use a statistical model for time series intervention analysis based on unobservable components. We use data from 2014 to 2018 for pre-policy model selection and the relatively short period up to September 2019 for early-stage policy assessment. We include weather conditions, socio-economic factors, and a counter-factual, given by the concentration of the same pollutant in other important neighbouring cities. Although the average concentrations reduced after the policy introduction, this paper argues that this could be due to other factors. Considering that the short time window may be not long enough for social adaptation to the new rules, our model does not provide statistical evidence of a positive policy effect for NO x and NO 2 . Instead, in one of the most central monitoring stations, a significant negative impact is found.
Teleconsultations between Patients and Healthcare Professionals in Primary Care in Catalonia: The Evaluation of Text Classification Algorithms Using Supervised Machine Learning
Authors: López Seguí Francesc , Ander Egg Aguilar Ricardo , de Maeztu Gabriel , García-Altés Anna , García Cuyàs Francesc , Walsh Sandra , Sagarra Castro Marta , Vidal-Alaball Josep
Published: February 2020

Background: The primary care service in Catalonia has operated an asynchronous teleconsulting service between GPs and patients since 2015 (eConsulta), which has generated some 500,000 messages. New developments in big data analysis tools, particularly those involving natural language, can be used to accurately and systematically evaluate the impact of the service. Objective: The study was intended to assess the predictive potential of eConsulta messages through different combinations of vector representation of text and machine learning algorithms and to evaluate their performance. Methodology: Twenty machine learning algorithms (based on five types of algorithms and four text representation techniques) were trained using a sample of 3559 messages (169,102 words) corresponding to 2268 teleconsultations (1.57 messages per teleconsultation) in order to predict the three variables of interest (avoiding the need for a face-to-face visit, increased demand and type of use of the teleconsultation). The performance of the various combinations was measured in terms of precision, sensitivity, F-value and the ROC curve. Results: The best-trained algorithms are generally effective, proving themselves to be more robust when approximating the two binary variables “avoiding the need of a face-to-face visit” and “increased demand” (precision = 0.98 and 0.97, respectively) rather than the variable “type of query” (precision = 0.48). Conclusion: To the best of our knowledge, this study is the first to investigate a machine learning strategy for text classification using primary care teleconsultation datasets. The study illustrates the possible capacities of text analysis using artificial intelligence. The development of a robust text classification tool could be feasible by validating it with more data, making it potentially more useful for decision support for health professionals.
Factors Impacting on Development and Implementation of Training Programs for Health Professionals to Deliver Brief Interventions, with a Focus on Programs Developed for Indigenous Clients: A Literature Review
Authors: Sebastian Saji , Thomas David P. , Brimblecombe Julie , Majoni Vongayi , Cunningham Frances C.
Published: February 2020

This paper reviews the literature on evaluations of brief intervention training programs for health professionals which address one or more lifestyle factors of chronic disease to identify factors impacting on development and implementation of programs. A search was conducted of the literature evaluating brief intervention training programs from 2000–2019 in the databases: Medline, CINAHL, Psychinfo, Academic Premier, Science Direct, Ovid (Including EMBASE and Healthstar), Web of Science and Informit. The content analysis and data extraction were aligned to the domains in the Consolidated Framework for Implementation Research (CFIR) to assist in the narrative synthesis. The search identified eight evaluations of programs targeting multiple risk factors, and 17 targeting single risk factors. The behavioural risk factor most commonly addressed was smoking, followed by alcohol and drug use. Programs consisted of face-to-face workshops and/or online or distance learning methods. Facilitators included availability of sustainable funding, adapting the program to suit the organisation’s structural characteristics and adoption of the intervention into routine client care. For Indigenous programs, the use of culturally appropriate images and language, consultation with Indigenous communities, and development of resources specific to the communities targeted were important considerations.
Assessment of the Nutrient Removal Potential of Floating Native and Exotic Aquatic Macrophytes Cultured in Swine Manure Wastewater
Authors: Xu Lei , Cheng Siyu , Zhuang Ping , Xie Dongsheng , Li Shiyu , Liu Dongming , Li Zhian , Wang Faguo , Xing Fuwu
Published: February 2020

Although eutrophication and biological invasion have caused serious harm to aquatic ecosystems, exotic and even invasive plants have been used extensively in phytoremediation water systems in China. To identify native aquatic plants with excellent water restoration potential, two representative native floating aquatic plants from Guangdong Province, namely Ludwigia adscendens (PL) and Trapa natans (PT), were selected, with Eichhornia crassipes as a control, to study their growth status, adaptability, and nutrient removal potentials in swine manure wastewater. The results demonstrated that the two native plants offered greater advantages than E. crassipes in water restoration. Within 60 days, PL and PT exhibited excellent growth statuses, and their net biomass growth rates were 539.8% and 385.9%, respectively, but the E. crassipes decayed and died with an increasing HRT (hydraulic retention time). The PL and PT could adjust the pH of the wastewater, improve the dissolved oxygen and oxidation-reduction potential, and reduce the electrical conductivity value. The removal rates of NH4+–N, NO3−–N, NO2−–N, total nitrogen, total phosphorus, chemical oxygen demand (COD), and Chl-a in the PL group reached 98.67%, 64.83%, 26.35%, 79.30%, 95.90%, 69.62%, and 92.23%, respectively; those in the PT group reached 99.47%, 95.83%, 85.17%, 83.73%, 88.72%, 75.06%, and 91.55%, respectively. The absorption contribution rates of total nitrogen (TN) and total phosphorus (TP) in the PL group were 40.6% and 43.5%, respectively, while those in the PT group were 36.9% and 34.5%, respectively. The results indicated that L. adscendens and T. natans are both promising aquatic plants for application to the restoration of swine manure wastewater in subtropical areas.
Prototyping a Social Media Flooding Photo Screening System Based on Deep Learning
Authors: Ning Huan , Li Zhenlong , Hodgson Michael E. , Wang Cuizhen (Susan)
Published: February 2020

This article aims to implement a prototype screening system to identify flooding-related photos from social media. These photos, associated with their geographic locations, can provide free, timely, and reliable visual information about flood events to the decision-makers. This screening system, designed for application to social media images, includes several key modules: tweet/image downloading, flooding photo detection, and a WebGIS application for human verification. In this study, a training dataset of 4800 flooding photos was built based on an iterative method using a convolutional neural network (CNN) developed and trained to detect flooding photos. The system was designed in a way that the CNN can be re-trained by a larger training dataset when more analyst-verified flooding photos are being added to the training set in an iterative manner. The total accuracy of flooding photo detection was 93% in a balanced test set, and the precision ranges from 46–63% in the highly imbalanced real-time tweets. The system is plug-in enabled, permitting flexible changes to the classification module. Therefore, the system architecture and key components may be utilized in other types of disaster events, such as wildfires, earthquakes for the damage/impact assessment.
Impaired Innate Immunity Mechanisms in the Brain of Alzheimer’s Disease
Authors: Romagnoli Martina , Porcellini Elisa , Carbone Ilaria , Veerhuis Robert , Licastro Federico
Published: February 2020

Among environmental factors likely associated with Alzheimer’s disease (AD), persistent virus infections, and age-related progressive decline of immune competence might play a pivotal role. However, AD antimicrobial brain immune responses are poorly investigated. The present study focused on genes involved in antimicrobial defenses, especially against virus infections, in the AD brain. In particular, mRNA levels of IRF7, MED23, IL28B, and IFN-α genes were analyzed in hippocampus and temporal cortex brain samples from AD and non-demented controls. All subjects were also genotyped for APOE ε, IRF7, MED23, and IL28B gene polymorphisms. Most AD patients showed decreased mRNA levels of all investigated genes in the hippocampus and temporal cortex. However, a small group of AD patients showed increased hippocampal mRNA expression of MED23, IL28B, and IFN-α. mRNA levels of MED23, IL28B, IFN-α from the hippocampus and those of MED23 from the temporal cortex were further decreased in APOE ε4 allele AD carriers. Moreover, rs6598008 polymorphism of IRF7 was significantly associated with decreased hippocampal expression of IRF7, MED23, IL28B, and IFN-α. These findings suggest that AD brains show impaired innate antimicrobial gene expression profiles, and individual genetic makeup, such as positivity for the APOE ε4 and IRF7 A alleles, might affect brain immune efficiency.
Processed Food Additive Microbial Transglutaminase and Its Cross-Linked Gliadin Complexes Are Potential Public Health Concerns in Celiac Disease
Authors: Lerner Aaron , Matthias Torsten
Published: February 2020

Microbial transglutaminase (mTG) is a survival factor for microbes, but yeasts, fungi, and plants also produce transglutaminase. mTG is a cross-linker that is heavily consumed as a protein glue in multiple processed food industries. According to the manufacturers’ claims, microbial transglutaminase and its cross-linked products are safe, i.e., nonallergenic, nonimmunogenic, and nonpathogenic. The regulatory authorities declare it as “generally recognized as safe” for public users. However, scientific observations are accumulating concerning its undesirable effects on human health. Functionally, mTG imitates its family member, tissue transglutaminase, which is the autoantigen of celiac disease. Both these transglutaminases mediate cross-linked complexes, which are immunogenic in celiac patients. The enzyme enhances intestinal permeability, suppresses mechanical (mucus) and immunological (anti phagocytic) enteric protective barriers, stimulates luminal bacterial growth, and augments the uptake of gliadin peptide. mTG and gliadin molecules are cotranscytosed through the enterocytes and deposited subepithelially. Moreover, mucosal dendritic cell surface transglutaminase induces gliadin endocytosis, and the enzyme-treated wheat products are immunoreactive in CD patients. The present review summarizes and updates the potentially detrimental effects of mTG, aiming to stimulate scientific and regulatory debates on its safety, to protect the public from the enzyme’s unwanted effects.
IL-33 Inhibits TNF-α-Induced Osteoclastogenesis and Bone Resorption
Authors: Ohori Fumitoshi , Kitaura Hideki , Ogawa Saika , Shen Wei-Ren , Qi Jiawei , Noguchi Takahiro , Marahleh Aseel , Nara Yasuhiko , Pramusita Adya , Mizoguchi Itaru
Published: February 2020

Interleukin (IL)-33 is a member of the IL-1 family, which acts as an alarmin. Several studies suggested that IL-33 inhibited osteoclastogenesis and bone resorption. Tumor necrosis factor-α (TNF-α) is considered a direct inducer of osteoclastogenesis. However, there has been no report regarding the effect of IL-33 on TNF-α-induced osteoclastogenesis and bone resorption. The objective of this study is to investigate the role of IL-33 on TNF-α-induced osteoclastogenesis and bone resorption. In an in vitro analysis of osteoclastogenesis, osteoclast precursors, which were derived from bone marrow cells, were treated with or without IL-33 in the presence of TNF-α. Tartrate-resistant acid phosphatase (TRAP) staining solution was used to assess osteoclast formation. In an in vivo analysis of mouse calvariae, TNF-α with or without IL-33 was subcutaneously administrated into the supracalvarial region of mice daily for 5 days. Histological sections were stained for TRAP, and osteoclast numbers were determined. Using micro-CT reconstruction images, the ratio of bone destruction area on the calvariae was evaluated. The number of TRAP-positive cells induced by TNF-α was significantly decreased with IL-33 in vitro and in vivo. Bone resorption was also reduced. IL-33 inhibited IκB phosphorylation and NF-κB nuclear translocation. These results suggest that IL-33 inhibited TNF-α-induced osteoclastogenesis and bone resorption.
Arginase Inhibition Supports Survival and Differentiation of Neuronal Precursors in Adult Alzheimer’s Disease Mice
Authors: Polis Baruh , Srikanth Kolluru D. , Gurevich Vyacheslav , Bloch Naamah , Gil-Henn Hava , Samson Abraham O.
Published: February 2020

Adult neurogenesis is a complex physiological process, which plays a central role in maintaining cognitive functions, and consists of progenitor cell proliferation, newborn cell migration, and cell maturation. Adult neurogenesis is susceptible to alterations under various physiological and pathological conditions. A substantial decay of neurogenesis has been documented in Alzheimer’s disease (AD) patients and animal AD models; however, several treatment strategies can halt any further decline and even induce neurogenesis. Our previous results indicated a potential effect of arginase inhibition, with norvaline, on various aspects of neurogenesis in triple-transgenic mice. To better evaluate this effect, we chronically administered an arginase inhibitor, norvaline, to triple-transgenic and wild-type mice, and applied an advanced immunohistochemistry approach with several biomarkers and bright-field microscopy. Remarkably, we evidenced a significant reduction in the density of neuronal progenitors, which demonstrate a different phenotype in the hippocampi of triple-transgenic mice as compared to wild-type animals. However, norvaline showed no significant effect upon the progenitor cell number and constitution. We demonstrated that norvaline treatment leads to an escalation of the polysialylated neuronal cell adhesion molecule immunopositivity, which suggests an improvement in the newborn neuron survival rate. Additionally, we identified a significant increase in the hippocampal microtubule-associated protein 2 stain intensity. We also explore the molecular mechanisms underlying the effects of norvaline on adult mice neurogenesis and provide insights into their machinery.
Using Probiotics as Supplementation for Helicobacter pylori Antibiotic Therapy
Authors: Ji Jianfu , Yang Hong
Published: February 2020

Helicobacter pylori is a well-known pathogen that is highly prevalent in the world population, and H. pylori infection is potentially hazardous to humans because of its relationship to various gastrointestinal diseases, such as gastric ulcers, chronic gastritis, and gastric carcinoma. Therefore, the clinical guidelines recommend taking antibiotic therapy to eradicate the pathogen, which usually leads to the desired therapeutic effect. However, some failure cases of this therapy indicate that the increasing antibiotic resistance and side effects may affect the therapeutic effect. Here we propose that using probiotics as supplementation for antibiotic therapy may provide an extra help. Recent studies have shown that probiotic supplementation therapy has promising application prospects; it can enhance the antibiotic effect to achieve a better therapeutic result and maintain the balance of the host gastrointestinal microbiota. In summary, under global conditions of increasing H. pylori prevalence, probiotic supplementation therapy is worthy of further studies for future clinical application.
Cell-Based Blood Biomarkers for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Authors: Missailidis Daniel , Sanislav Oana , Allan Claire Y. , Annesley Sarah J. , Fisher Paul R.
Published: February 2020

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a devastating illness whose biomedical basis is now beginning to be elucidated. We reported previously that, after recovery from frozen storage, lymphocytes (peripheral blood mononuclear cells, PBMCs) from ME/CFS patients die faster in culture medium than those from healthy controls. We also found that lymphoblastoid cell lines (lymphoblasts) derived from these PBMCs exhibit multiple abnormalities in mitochondrial respiratory function and signalling activity by the cellular stress-sensing kinase Target Of Rapamycin Complex 1 (TORC1). These differences were correlated with disease severity, as measured by the Richardson and Lidbury weighted standing test. The clarity of the differences between these cells derived from ME/CFS patient blood and those from healthy controls suggested that they may provide useful biomarkers for ME/CFS. Here, we report a preliminary investigation into that possibility using a variety of analytical classification tools, including linear discriminant analysis, logistic regression and receiver operating characteristic (ROC) curve analysis. We found that results from three different tests—lymphocyte death rate, mitochondrial respiratory function and TORC1 activity—could each individually serve as a biomarker with better than 90% sensitivity but only modest specificity vís a vís healthy controls. However, in combination, they provided a cell-based biomarker with sensitivity and specificity approaching 100% in our sample. This level of sensitivity and specificity was almost equalled by a suggested protocol in which the frozen lymphocyte death rate was used as a highly sensitive test to triage positive samples to the more time consuming and expensive tests measuring lymphoblast respiratory function and TORC1 activity. This protocol provides a promising biomarker that could assist in more rapid and accurate diagnosis of ME/CFS.
Caspase-6 Knockout in the 5xFAD Model of Alzheimer’s Disease Reveals Favorable Outcome on Memory and Neurological Hallmarks
Authors: Angel Ariel , Volkman Rotem , Royal Tabitha Grace , Offen Daniel
Published: February 2020

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder and is the most common form of dementia in the elderly. Caspases, a family of cysteine proteases, are major mediators of apoptosis and inflammation. Caspase-6 is considered to be an up-stream modulator of AD pathogenesis as active caspase-6 is abundant in neuropil threads, neuritic plaques, and neurofibrillary tangles of AD brains. In order to further elucidate the role of caspase-6 activity in the pathogenesis of AD, we produced a double transgenic mouse model, combining the 5xFAD mouse model of AD with caspase-6 knock out (C6-KO) mice. Behavioral examinations of 5xFAD/C6-KO double transgenic mice showed improved performance in spatial learning, memory, and anxiety/risk assessment behavior, as compared to 5xFAD mice. Hippocampal mRNA expression analyses showed significantly reduced levels of inflammatory mediator TNF-α, while the anti-inflammatory cytokine IL-10 was increased in 5xFAD/C6-KO mice. A significant reduction in amyloid-β plaques could be observed and immunohistochemistry analyses showed reduced levels of activated microglia and astrocytes in 5xFAD/C6-KO, compared to 5xFAD mice. Together, these results indicate a substantial role for caspase-6 in the pathology of the 5xFAD model of AD and suggest further validation of caspase-6 as a potential therapeutic target for AD.
Expanding Role of Ubiquitin in Translational Control
Authors: Dougherty Shannon E. , Maduka Austin O. , Inada Toshifumi , Silva Gustavo M.
Published: February 2020

The eukaryotic proteome has to be precisely regulated at multiple levels of gene expression, from transcription, translation, and degradation of RNA and protein to adjust to several cellular conditions. Particularly at the translational level, regulation is controlled by a variety of RNA binding proteins, translation and associated factors, numerous enzymes, and by post-translational modifications (PTM). Ubiquitination, a prominent PTM discovered as the signal for protein degradation, has newly emerged as a modulator of protein synthesis by controlling several processes in translation. Advances in proteomics and cryo-electron microscopy have identified ubiquitin modifications of several ribosomal proteins and provided numerous insights on how this modification affects ribosome structure and function. The variety of pathways and functions of translation controlled by ubiquitin are determined by the various enzymes involved in ubiquitin conjugation and removal, by the ubiquitin chain type used, by the target sites of ubiquitination, and by the physiologic signals triggering its accumulation. Current research is now elucidating multiple ubiquitin-mediated mechanisms of translational control, including ribosome biogenesis, ribosome degradation, ribosome-associated protein quality control (RQC), and redox control of translation by ubiquitin (RTU). This review discusses the central role of ubiquitin in modulating the dynamism of the cellular proteome and explores the molecular aspects responsible for the expanding puzzle of ubiquitin signals and functions in translation.
Understanding the Role of the Transcription Factor Sp1 in Ovarian Cancer: from Theory to Practice
Authors: Vellingiri Balachandar , Iyer Mahalaxmi , Devi Subramaniam Mohana , Jayaramayya Kaavya , Siama Zothan , Giridharan Bupesh , Narayanasamy Arul , Abdal Dayem Ahmed , Cho Ssang-Goo
Published: February 2020

Ovarian cancer (OC) is one of the deadliest cancers among women contributing to high risk of mortality, mainly owing to delayed detection. There is no specific biomarker for its detection in early stages. However, recent findings show that over-expression of specificity protein 1 (Sp1) is involved in many OC cases. The ubiquitous transcription of Sp1 apparently mediates the maintenance of normal and cancerous biological processes such as cell growth, differentiation, angiogenesis, apoptosis, cellular reprogramming and tumorigenesis. Sp1 exerts its effects on cellular genes containing putative GC–rich Sp1–binding site in their promoters. A better understanding of the mechanisms underlying Sp1 transcription factor (TF) regulation and functions in OC tumorigenesis could help identify novel prognostic markers, to target cancer stem cells (CSCs) by following cellular reprogramming and enable the development of novel therapies for future generations. In this review, we address the structure, function, and biology of Sp1 in normal and cancer cells, underpinning the involvement of Sp1 in OC tumorigenesis. In addition, we have highlighted the influence of Sp1 TF in cellular reprogramming of iPSCs and how it plays a role in controlling CSCs. This review highlights the drugs targeting Sp1 and their action on cancer cells. In conclusion, we predict that research in this direction will be highly beneficial for OC treatment, and chemotherapeutic drugs targeting Sp1 will emerge as a promising therapy for OC.
Chronic Mild Stress Modified Epigenetic Mechanisms Leading to Accelerated Senescence and Impaired Cognitive Performance in Mice
Authors: Puigoriol-Illamola Dolors , Martínez-Damas Mirna , Griñán-Ferré Christian , Pallàs Mercè
Published: February 2020

Cognitive and behavioural disturbances are a growing public healthcare issue for the modern society, as stressful lifestyle is becoming more and more common. Besides, several pieces of evidence state that environment is crucial in the development of several diseases as well as compromising healthy aging. Therefore, it is important to study the effects of stress on cognition and its relationship with aging. To address these queries, Chronic Mild Stress (CMS) paradigm was used in the senescence-accelerated mouse prone 8 (SAMP8) and resistant 1 (SAMR1). On one hand, we determined the changes produced in the three main epigenetic marks after 4 weeks of CMS treatment, such as a reduction in histone posttranslational modifications and DNA methylation, and up-regulation or down-regulation of several miRNA involved in different cellular processes in mice. In addition, CMS treatment induced reactive oxygen species (ROS) damage accumulation and loss of antioxidant defence mechanisms, as well as inflammatory signalling activation through NF-κB pathway and astrogliosis markers, like Gfap. Remarkably, CMS altered mTORC1 signalling in both strains, decreasing autophagy only in SAMR1 mice. We found a decrease in glycogen synthase kinase 3 β (GSK-3β) inactivation, hyperphosphorylation of Tau and an increase in sAPPβ protein levels in mice under CMS. Moreover, reduction in the non-amyloidogenic secretase ADAM10 protein levels was found in SAMR1 CMS group. Consequently, detrimental effects on behaviour and cognitive performance were detected in CMS treated mice, affecting mainly SAMR1 mice, promoting a turning to SAMP8 phenotype. In conclusion, CMS is a feasible intervention to understand the influence of stress on epigenetic mechanisms underlying cognition and accelerating senescence.
Objective Assessment of Counselling for Fetal Heart Defects: An Interdisciplinary Multicenter Study
Authors: Kovacevic Alexander , Bär Stefan , Starystach Sebastian , Simmelbauer Andreas , Elsässer Michael , Müller Andreas , Mohammadi Motlagh Aida , Oberhoffer-Fritz Renate , Ostermayer Eva , Ewert Peter , Gorenflo Matthias , Wacker-Gussmann Annette
Published: February 2020

The objective of this study was to analyze parental counselling for fetal heart disease in an interdisciplinary and multicenter setting using a validated questionnaire covering medical, sociodemographic, and psychological aspects. n = 168 individuals were recruited from two pediatric heart centers and two obstetrics units. Overall, counselling was combined successful and satisfying in >99%; only 0.7% of parents were dissatisfied. “Perceived situational control” was impaired in 22.6%. Adequate duration of counselling leads to more overall counselling success (r = 0.368 ***), as well as providing written or online information (57.7% vs. 41.5%), which is also correlated to more “Transfer of Medical Knowledge” (r = 0.261 ***). Interruptions of consultation are negatively correlated to overall counselling success (r = −0.247 **) and to “Transparency regarding the Treatment Process” (r = −0.227 **). Lacking a separate counselling room is associated with lower counselling success for “Transfer of Medical Knowledge” (r = 0.210 ***). High-risk congenital heart disease (CHD) is correlated to lower counselling success (42.7% vs. 71.4% in low-risk CHD). A lack of parental language skills leads to less overall counselling success. There is a trend towards more counselling success for “Transfer of Medical Knowledge” after being counselled solely by cardiologists in one center (r = 0.208). Our results indicate that a structured approach may lead to more counselling success in selected dimensions. For complex cardiac malformations, counselling by cardiologists is essential. Parental “Perceived Situational Control” is often impaired, highlighting the need for further support throughout the pregnancy.
Matrix Metalloproteinases in Diabetic Kidney Disease
Authors: Garcia-Fernandez Nuria , Jacobs-Cachá Conxita , Mora-Gutiérrez José María , Vergara Ander , Orbe Josune , Soler María José
Published: February 2020

Around the world diabetic kidney disease (DKD) is the main cause of chronic kidney disease (CKD), which is characterized by mesangial expansion, glomerulosclerosis, tubular atrophy, and interstitial fibrosis. The hallmark of the pathogenesis of DKD is an increased extracellular matrix (ECM) accumulation causing thickening of the glomerular and tubular basement membranes, mesangial expansion, sclerosis, and tubulointerstitial fibrosis. The matrix metalloproteases (MMPs) family are composed of zinc-dependent enzymes involved in the degradation and hydrolysis of ECM components. Several MMPs are expressed in the kidney; nephron compartments, vasculature and connective tissue. Given their important role in DKD, several studies have been performed in patients with DKD proposing that the measurement of their activity in serum or in urine may become in the future markers of early DKD. Studies from diabetic nephropathy experimental models suggest that a balance between MMPs levels and their inhibitors is needed to maintain renal homeostasis. This review focuses in the importance of the MMPs within the kidney and their modifications at the circulation, kidney and urine in patients with DKD. We also cover the most important studies performed in experimental models of diabetes in terms of MMPs levels, renal expression and its down-regulation effect.
Strength of the Association of Elevated Vitamin B12 and Solid Cancers: An Adjusted Case-Control Study
Authors: Urbanski Geoffrey , Hamel Jean-François , Prouveur Benoît , Annweiler Cédric , Ghali Alaa , Cassereau Julien , Lozac’h Pierre , Lavigne Christian , Lacombe Valentin
Published: February 2020

The association between elevated plasma vitamin B12 (B12) level and solid cancers has been documented by two national registries. However, their design did not allow for the adjustment for other conditions associated with elevated B12. The objectives of this study were to confirm this association after the adjustment for all causes of elevated B12, and to study the variations according to the increasing B12 level, the type of cancers, and the presence of metastases. We compared 785 patients with B12 ≥ 1000 ng/L with 785 controls matched for sex and age with B12 < 1000 ng/L. Analyses were adjusted for the causes of elevated B12: myeloid blood malignancies, acute or chronic liver diseases, chronic kidney failure, autoimmune or inflammatory diseases, and excessive B12 supplementation. A B12 ≥ 1000 ng/L was associated with the presence of solid cancer without metastases (OR 1.96 [95%CI: 1.18 to 3.25]) and with metastases (OR 4.21 [95%CI: 2.67 to 6.64]) after adjustment for all elevated B12-related causes. The strength of the association rose with the increasing B12 level, in particular in cases of metastases. No association between liver cancers and elevated B12 level was found after adjustment for chronic liver diseases. In conclusion, unexplained elevated B12 levels should be examined as a possible marker of solid cancer.
A Review of Game Theory Applications for Seaport Cooperation and Competition
Authors: Pujats Karlis , Golias Mihalis , Konur Dinçer
Published: February 2020

In the ever-changing maritime shipping industry, ports, and more specifically, container terminals, are always on the search for better policy and operational plan developments. As the maritime shipping sector changes, new areas of research emerge. In this paper, we present a review of recent game theory applications for seaport cooperation and competition, accompanied by summary review tables stating the study, the modeling technique used, the methodology and objective, and summary of the results of each study. In total, we have reviewed 33 studies that used game theory models that investigated seaport and container terminal competition and cooperation involving various stakeholders with dating publication years from 2008 to 2019. The paper concludes with a discussion and proposed future research directions. The purpose of the paper is to serve as a reference guide to recent game theory applications on seaport cooperation and competition that would allow a quick understanding of work done in the field.
Sampling Procedure for Establishing Criteria for Sea Urchin (Loxechinus albus) Proportion under the Legal Minimum Length of landings
Authors: Di Giorgi Gustavo , Querales Marvin , Contreras-Reyes Javier E. , Pereira Danilo , Hernández-Santoro Carola
Published: February 2020

Sea urchin (Loxechinus albus) is one of the most economically important species in the northeast of Chilean Patagonia, forming part of the highly diverse benthic community. This resource is being harvested under selective fishing pressure, which suppresses growth rates. In response, the National Standards Institute established Regulation 44 as a quality control measure, based on a sampling approach considering the percentage of sea urchin under the minimum legal size (UMLS) through an acceptance sampling plan. In 2019, the Chilean Undersecretary of Fisheries and Aquaculture (SUBPESCA), under Exempt Resolution No. 910, ruled that any vessel that landed 15% with sea urchin of 6.5 cm UMLS of the total catch weight will be sanctioned. In this paper, a sampling design is presented to establish criteria regarding compliance with this provision. The plan provides a statistical methodology for the evaluation of the percentage of UMLS sea urchin, which corroborates and certifies the current legislation that sanctions and fines the capture of sea urchin that does not comply with UMLS values outlined in Regulation 44. Assuming a systematic sampling plan and under assumed UMLS values of 6.0, 6.5, and 7.0 cm, it is determined that 15% of total landings (margin of tolerance) of sea urchin under 6.5 cm is the optimal level. Thus, a correct specification of the UMLS allows protecting the most vulnerable fraction of the population from overexploitation for growth.
Investigating Polymer Fibre Optics for Condition Monitoring of Synthetic Mooring Lines
Authors: Gordelier Tessa , Thies Phillip Rudolph , Rinaldi Giovanni , Johanning Lars
Published: February 2020

Synthetic mooring lines are becoming a popular alternative to conventional chain mooring systems. For marine renewable energy devices, they have been considered as an enabling technology for this nascent sector, given their reduced costs and ease of deployment. However, the extreme operating environment has led to an increased interest in the ‘in-situ’ condition monitoring of these mooring lines. This paper considers the use of polymer fibre optic technology and the optical time domain reflectometry (OTDR) technique for the condition monitoring of synthetic mooring lines. To establish the operating envelope of the fibres, Polymethylmethacrylate (PMMA) polymer optical fibres are mechanically tested. Additionally, an OTDR is used to monitor fibres whilst under elongation using a tensile test machine, and the sensitivity of the system in monitoring strain is established. At the lowest strain rate, the average proportional limit and yield points of the fibres are found at 1.16% strain and 5.41% strain, respectively. Fatigue exposure of fibres up to 1.25% strain identifies no measurable effect on fibres’ proportional limit or yield point. The occurrence of significant creep is identified for fibres strained beyond 1.5%. The OTDR system is able to identify strains at and above 4%. The study identifies important criteria that should be considered in the integration of polymer optical fibres for mooring applications. Limitations are discussed and suggestions for progressing this technology are provided.
Improving Quality-Of-Service in LoRa Low-Power Wide-Area Networks through Optimized Radio Resource Management
Authors: Sallum Eduardo , Pereira Nuno , Alves Mário , Santos Max
Published: February 2020

Low Power Wide Area Networks (LPWAN) enable a growing number of Internet-of-Things (IoT) applications with large geographical coverage, low bit-rate, and long lifetime requirements. LoRa (Long Range) is a well-known LPWAN technology that uses a proprietary Chirp Spread Spectrum (CSS) physical layer, while the upper layers are defined by an open standard—LoRaWAN. In this paper, we propose a simple yet effective method to improve the Quality-of-Service (QoS) of LoRaWAN networks by fine-tuning specific radio parameters. Through a Mixed Integer Linear Programming (MILP) problem formulation, we find optimal settings for the Spreading Factor (SF) and Carrier Frequency (CF) radio parameters, considering the network traffic specifications as a whole, to improve the Data Extraction Rate (DER) and to reduce the packet collision rate and the energy consumption in LoRa networks. The effectiveness of the optimization procedure is demonstrated by simulations, using LoRaSim for different network scales. In relation to the traditional LoRa radio parameter assignment policies, our solution leads to an average increase of 6% in DER, and a number of collisions 13 times smaller. In comparison to networks with dynamic radio parameter assignment policies, there is an increase of 5%, 2.8%, and 2% of DER, and a number of collisions 11, 7.8 and 2.5 times smaller than equal-distribution, Tiurlikova’s (SOTA), and random distribution, respectively. Regarding the network energy consumption metric, the proposed optimization obtained an average consumption similar to Tiurlikova’s, and 2.8 times lower than the equal-distribution and random dynamic allocation policies. Furthermore, we approach the practical aspects of how to implement and integrate the optimization mechanism proposed in LoRa, guaranteeing backward compatibility with the standard protocol.
Protected Areas as a Center of Attraction for Visits from World Heritage Cities: Extremadura (Spain)
Authors: Sánchez-Martín José-Manuel , Rengifo-Gallego Juan-Ignacio , Sánchez-Rivero Marcelino
Published: February 2020

Conservation of the environment has become a key factor in tourist development, as is shown by the increase in visitors to natural parks and other places with rich ecosystems. Protected areas have become polarised centers for tourists of very varied characteriztics, not only for those who make them their main destination, but also for those who travel to nearby areas. This situation can be observed in Extremadura, where numerous tourists make the best use of their stay in the main centers of tourist attractions of the region to make local trips and thus get to know the most significant natural areas. These movements are being detected in varied aspects of demand, sometimes with little connection between the main and secondary motivation for the trip. We therefore consider a variable percentage of tourists visiting the main cultural destinations of Extremadura who use part of their stay to get to know protected areas. With the aim of structuring the research, we made a systematic study of tourists who spent the night in the cities of Cáceres and Mérida, which are World Heritage sites. During their stay, they visited prestigious natural places such as Monfragüe National Park, the Villuercas–Ibores–Jara Geopark, the Tajo Internacional Nature Reserve, and the Garganta de los Infiernos Nature Reserve. This information was obtained by carrying out surveys, which allowed us to determine the attraction capacity of each protected area by applying a network analysis. The results reflect a heterogeneous type of visitor who travels to the most appreciated areas following guidelines marked by the time of the year, his/her preferences, and the duration of his/her trip.
β-Cyclodextrin-Immobilized Ni/Graphene Electrode for Electrochemical Enantiorecognition of Phenylalanine
Authors: Chen Feiyue , Fan Zhiqin , Zhu Yangguang , Sun Huifang , Yu Jinhong , Jiang Nan , Zhao Shichao , Lai Guosong , Yu Aimin , Lin Cheng-Te , Ye Chen , Fu Li
Published: February 2020

In this work, a Ni/graphene (Ni/G) electrode was designed and fabricated by plasma-enhanced chemical vapor deposition (PECVD) for the ultrasensitive recognition of d- and l-phenylalanine. Through a single-step PECVD process, the Ni/G electrode can achieve better hydrophilicity and larger catalytic surface area, which is beneficial for the electrochemical recognition of bio-objects. After surface modification with β-cyclodextrin, the Ni/G electrode can distinguish d-phenylalanine from l-phenylalanine according to a 0.09 V peak shift in differential pulse voltammetry tests. Moreover, this Ni/G electrode achieved a detection limit as low as 1 nM and a wide linear range from 1 nM to 10 mM toward l-phenylalanine, with great storage stability and working stability.
Dynamic Fracture and Fragmentation Characteristics of Metal Cylinder and Rings Subjected to Internal Explosive Loading
Authors: An Xuanyi , Ye Ping , Liu Jiayun , Tian Chao , Feng Shunshan , Dong Yongxiang
Published: February 2020

Dynamic fracture and fragmentation characteristics of explosively driven rings and cylinders are important issues in the field of weapon effectiveness and protection. However, the comparison of fracture characteristics between metal cylinder and rings, and the fracture characteristics of the metal shells at different axial positions, are rarely touched. In the present work, a recovery tank was used to collect fragments, and witness plates were used to investigate the fragment spatial distributions. Before the test, the representative positions of metal shells were plated with copper layers to locate the original position of the recovered fragments. After the test, scanning electron microscopy and optical microscope were used for characterizing the microstructure of the recovered fragments from different positions. Then, the recovered fragments were weighed and measured to investigate their mass and size characteristics. In addition, numerical simulation was used to further investigate the fracture mechanisms of explosively driven cylinders and rings. It was found that the projection angle axial distribution of the fragments for the metal cylinder was similar to that of the fragments for the metal rings. However, the fracture characteristics of the metal rings were significantly different from those of the metal cylinder. The adiabatic shear band played a key role in the fracture process of the metal cylinder, whereas the adiabatic shear band had little chance to initiate in the fracture process of the metal rings because the metal rings could deform uniformly with much fewer strain localizations due to their much lower length. The fracture surfaces of the fragments from different positions of the metal cylinder were very smooth, whereas dimples were found in the fracture surfaces of the fragments from different positions of the metal rings. The mass distribution of the fragments from the metal rings was more uniform than that of the fragments from the metal cylinder, and the circumferential rupture strains of the metal rings were much larger than those of the metal cylinder.
Maintenance of the Austenite/Ferrite Ratio Balance in GTAW DSS Joints Through Process Parameters Optimization
Authors: Rodriguez Bryan R. , Miranda Argelia , Gonzalez David , Praga Rolando , Hurtado Eduardo
Published: February 2020

The present work describes the influence of the parameters employed in the gas tungsten arc welding process (GTAW) when nickel powder is used as a filler metal in 2304/2507 duplex stainless-steel dissimilar joints. Multi-objective optimization was applied in order to maintain the austenite/ferrite percentage in the welded zone. A microstructural and phase quantification analysis was performed in each sample through optical and scanning electron microscopes. It was found that a nickel powder addition combined with low heat input increased the biphasic ratio across the different zones of the dissimilar welded samples. Although the austenite volume fraction increased in the 2304 heat-affected zone (HAZ) near to 25%, it was not sufficient according to international standards. The obtained results led to the maintenance of the 50/50 phase percentage in the 2507 HAZ welded joint side, as well as to the increment of the austenite percentage in the 2304 HAZ.
Numerical Solutions of Fractional Differential Equations Arising in Engineering Sciences
Authors: Jannelli Alessandra
Published: February 2020

This paper deals with the numerical solutions of a class of fractional mathematical models arising in engineering sciences governed by time-fractional advection-diffusion-reaction (TF–ADR) equations, involving the Caputo derivative. In particular, we are interested in the models that link chemical and hydrodynamic processes. The aim of this paper is to propose a simple and robust implicit unconditionally stable finite difference method for solving the TF–ADR equations. The numerical results show that the proposed method is efficient, reliable and easy to implement from a computational viewpoint and can be employed for engineering sciences problems.
Multiscale Compression Algorithm for Solving Nonlinear Ill-Posed Integral Equations via Landweber Iteration
Authors: Zhang Rong , Li Fanchun , Luo Xingjun
Published: February 2020

In this paper, Landweber iteration with a relaxation factor is proposed to solve nonlinear ill-posed integral equations. A compression multiscale Galerkin method that retains the properties of the Landweber iteration is used to discretize the Landweber iteration. This method leads to the optimal convergence rates under certain conditions. As a consequence, we propose a multiscale compression algorithm to solve nonlinear ill-posed integral equations. Finally, the theoretical analysis is verified by numerical results.
Certain Fractional Proportional Integral Inequalities via Convex Functions
Authors: Rahman Gauhar , Nisar Kottakkaran Sooppy , Abdeljawad Thabet , Ullah Samee
Published: February 2020

The goal of this article is to establish some fractional proportional integral inequalities for convex functions by employing proportional fractional integral operators. In addition, we establish some classical integral inequalities as the special cases of our main findings.