bluuebunny/crossref_metadata_2025 huggingface dataset:
Integration of PAI Learning Methods Based on Sorogan and Discovery Learning at Khadijah Senior High School Surabaya
Authors: Ahmad Wildan Saifullah Alaina , Uroidli Ali , Ayu Cahya Ningrum Annisa Eka , Fahmi Yusril Fahmi , Fuad Ah. Zakki
Published: July 2024

PAI teachers' efforts to develop learning both outside and inside the classroom are very varied, especially in maximizing digital media to support increased students' understanding. The urgency of using digital in learning is something that every teacher naturally has, because the teacher's ability to use digital in learning can help the learning methods used by teachers become more varied and not monotonous. Especially at Khadijah High School which has really maximized media in its learning, of course the sorogan and discovery learning methods that are applied will not be able to work if it is not accompanied by the teacher's ability to utilize media for learning, in addition to the teacher's ability to master devices, media and the use of learning methods, Teachers must also have content and materials, so that they all support the learning process and increase understanding not only of students' cognitive, but also affective improvement. In this research, we will examine further the integration of sorogan and discovery learning methods at SMA Khadijah Surabaya.
EVALUATION OF THE INDEPENDENT CURRICULUM IMPLEMENTATION PROGRAM IN PAI LEARNING
Authors: St Jumaeda  , Rahmat Abidin Andi
Published: July 2024

Tujuan penelitian ini adalah untuk mengevaluasi implementasi kurikulum merdeka pada pembelajaran Pendidikan Agama Islam di SD Negeri 2 Tual melihat dari aspek konteks, Input, proses dan produk. Jenis penelitian ini adalah penelitian evaluasi yang dikembangkan oleh Stuffelebeam, yang mencakup empat komponen utama, yaitu: (konteks, input, proses dan produk) dengan pendekatan kualitatif. Subyek penelitian terdiri dari kepala sekolah, guru PAI dan peserta didik. Teknik pengumpulan data menggunakan observasi, wawancara dan dokumentasi. Hasil penelitian menunjukkan bahwa implementasi kurikulum merdeka pada pembelajaran Pendidikan Agama Islam di SD Negeri 2 Tual secara keseluruhan telah berjalan dengan baik. Pada tahap konteks, kurikulum merdeka telah disusun dengan memperhatikan kebutuhan dan karakteristik siswa. Dari segi input, pihak sekolah telah menyiapkan sarana dan prasarana yang memadai serta sumber daya guru dan tenaga kependidikan yang kompeten dalam menerapkan kurikulum merdeka. Dalam tahap proses, pelaksanaan pembelajaran PAI menggunakan metode yang variatif dan berpusat pada siswa. Pada tahap produk, hasil belajar siswa dalam mata pelajaran PAI menunjukkan peningkatan yang signifikan setelah penerapan kurikulum merdeka. Meskipun demikian, masih terdapat beberapa aspek yang perlu dioptimalkan, seperti sosialisasi kurikulum merdeka kepada orang tua siswa dan peningkatan kompetensi guru dalam merancang pembelajaran inovatif serta partisipasi aktif siswa dalam pembelajaran masih merupakan aspek yang dapat ditingkatkan.
TM6SF2 E167K variant decreases PNPLA3-mediated PUFA transfer to promote hepatic steatosis and injury in MASLD
Authors: Sun Baokai , Ding Xiaoqian , Tan Jie , Zhang Jie , Chu Xueru , Zhang Shuimi , Liu Shousheng , Zhao Zhenzhen , Xuan Shiying , Xin Yongning , Zhuang Likun
Published: July 2024

Backgrounds/Aims: Transmembrane 6 superfamily member 2 (TM6SF2) E167K variant is closely associated with the occurrence and development of metabolic dysfunction-associated steatotic liver disease (MASLD). However, the role and mechanism of TM6SF2 E167K variant during MASLD progression are not yet fully understood.Methods: The Tm6sf2167K knock-in (KI) mice were subjected to high-fat diet (HFD). Hepatic lipid levels of Tm6sf2167K KI mice were detected by lipidomics analysis. Thin-layer chromatography (TLC) was used to measure the newly synthesized triglyceride (TG) and phosphatidylcholine (PC).Results: The TM6SF2 E167K variant significantly aggravated hepatic steatosis and injury in HFD-induced mice. Decreased polyunsaturated PC level and increased polyunsaturated TG level were found in liver tissue of HFD-induced Tm6sf2167K KI mice. Mechanistic studies demonstrated that the TM6SF2 E167K variant increased the interaction between TM6SF2 and PNPLA3, and impaired PNPLA3-mediated transfer of polyunsaturated fatty acids (PUFAs) from TG to PC. The TM6SF2 E167K variant increased the level of fatty acid-induced malondialdehyde and reactive oxygen species, and decreased fatty acid-downregulated cell membrane fluidity. Additionally, the TM6SF2 E167K variant decreased the level of hepatic PC containing C18:3, and dietary supplementation of PC containing C18:3 significantly attenuated the TM6SF2 E167K-induced hepatic steatosis and injury in HFD-fed mice.Conclusions: The TM6SF2 E167K variant could promote its interaction with PNPLA3 and inhibit PNPLA3-mediated transfer of PUFAs from TG to PC, resulting in the hepatic steatosis and injury during MASLD progression. PC containing C18:3 could act as a potential therapeutic supplement for MASLD patients carrying the TM6SF2 E167K variant.
CAN IMPRESSION MANAGEMENT MODERATE THE RELATIONSHIP BETWEEN FINANCIAL PERFORMANCE AND COST OF EQUITY?
Authors:   Universitas Sebelas Maret, Santoso Arif , Rahayu Selvia ,   Faculty of Economics and Business, Universitas Negeri Semarang, Sari Puji Novita ,   Faculty of Teacher Training and Education, Universitas Sebelas Maret
Published: July 2024

Stock market financing access plays an important role in sustainable business resilience and financial performance. In the Covid-19 pandemic, the banking sector has the potential to bear high equity costs commensurate with the risk. Impression management as a corporate communication strategy can maintain shareholder perception of corporate risk. The study aims to examine the effect of impression management in moderating the relationship between financial performance and cost of equity. The study consists of 228 bank-year observations on the Southeast Asian stock exchanges for 2020-2022. Data was obtained from the Annual Report, Integrated Report, and Form 56-1 One Report. This study examines separate companies that use one-tier and two-tier board systems. The findings indicate that improvements in financial performance had a negative impact on equity costs in Indonesia but a positive impact in Thailand, Singapore, Malaysia, and the Philippines. ROA and ROE only had a beneficial influence on Indonesia. The independent Board’s relationship to the cost of equity was not demonstrated. This study supports the idea that impression management improves the relationship between pandemic-related changes in financial performance and the cost of equity. The practical implications are that companies with negative changes in performance that also have proper impression management can attract investors with lower equity costs.
Manajemen Bimbingan dan Konseling dalam Peningkatan Karater Siswa di MTs Negeri 9 Jombang
Authors: Azis Ixora chinensis , Asep Kurniawan
Published: July 2024

Kegiatan Bimbingan dan konseling memiliki peran yang sangat penting dalam meningkatkan karakter siswa di madrasah. Oleh karena itu, penting untuk mengatur dan mengelola kegiatan tersebut secara terstruktur dengan menerapkan fungsi-fungsi manajemen. Tujuan penelitian ini adalah untuk: 1) mengetahui manajemen bimbingan dan konseling di MTs Negeri 9 Jombang, 2) mengetahui  karakter siswa di MTs Negeri 9 Jombang, dan 3) mengetahui manajemen bimbingan dan konseling dalam peningkatan karakter siswa di MTs Negeri 9 Jombang. Penelitian ini menggunakan penelitian kualitatif dengan pendekatan studi kasus. Adapun teknik pengumpulan data menggunakan observasi, wawancara & dokumentasi. Hasil penelitian ini menunjukkan bahwa 1) Manajemen bimbingan dan konseling di MTs Negeri 9 Jombang berjalan dengan cukup baik dan efektif, ditunjukkan oleh sistem dan penyusunan program yang baik dan terorganisir, dilengkapi dengan fasilitas yang mendukung, serta perencanaan yang tepat. 2) Karakter siswa di MTs Negeri 9 Jombang Karakter siswa-siswi di MTs Negeri 9 sudah dapat dikatakan baik. Namun, masih ada sebagian kecil siswa-siswi yang menunjukkan karakter kurang baik. Upaya peningkatan karakter di MTs Negeri 9 Jombang menggunakan 2 model, yaitu habituasi dan intervensi. 3) Bimbingan dan konseling dalam peningkatan karakter siswa memiliki dampak positif terhadap kegiatan pengajaran, tingkah laku siswa, dan prestasi belajar siswa.
Tanggungjawab Pelaku Usaha Terhadap Pencantuman Tanggal Kadaluwarsa pada produk ASPERA Jombang Perspektif Etika Bisnis Syari’ah
Authors: Hikmah Dewi Latifatul , Fitria Norma
Published: July 2024

Penelitian ini bertujuan untuk menganalisis Tanggungjawab Pelaku Usaha  Terhadap Pencantuman Tanggal Kadaluwarsa Pada Produk Asosiasi ASPERA Jombang Perpektif Etika Bisnis Syari’ah. Penelitian ini menggunakan metode penelitian Yuridis Empiris dengan pendekatan Conseptual Aproach, serta pengumpulan datanya menggunakan observasi, wawancara, dokumentasi, dan data kepustakaan. Data yang diperoleh kemudian dianalisis dengan menggunakan metode induktif dan deskriptif. Berdasarkan hasil penelitian diperoleh bahwa: 1) Pencantuman Tanggal Kadaluwarsa pada para Pelaku Usaha  Samiler Asosiasi ASPERA. 2) Etika Pelaku Usaha  ASPERA berdasarkan teori Etika Bisnis Syari’ah.
The Effect of Low-Impact Aerobic Exercise on Reducing Stress Levels in the Elderly
Authors: Pratiwi Gilang Dwi , Lucya Vita , Hadiyani Wini
Published: July 2024

Aims: Increasing a person's age results in a significant decline in body development, which is one of the causes of stress conditions in the elderly. In addition to a decrease in physical condition, the elderly who experience a decrease in social activities, one of which is the state of retirement. The elderly who used to work experienced drastic changes in their activities. There needs to be special treatment that can divert the stress experienced by the elderly rather not get worse. This study aims to identify the effect of low-impact aerobic exercise on reducing stress levels in the elderly.
Methods: This research is a quasi-experimental research (One Group Pre-Post-test design), sampling using purposive sampling of 30 respondents. The population in this study is the elderly in the Antapani District. The measurement tool used is the PSS-10 (Perceived Stress Scale) questionnaire.
Results: The research results show that the majority of respondents in this study were female, with an average age of 60 years. Then on the Wilcoxon test there is a difference in the average value of the pre-test and post-test and the difference is quite large.
Conclusion: There is an effect of low-impact aerobic exercise on reducing stress levels in the elderly. Suggestion: It is hoped that this exercise will be carried out regularly once a week. This is because in addition to body fitness, and to eliminate boredom, it can also reduce stress in the elderly.
Nursing Intervention Toward Quality of Life of Patients Undergoing Hemodialysis: A Systematic Review
Authors: Susanto Gunawan , Upoyo Arif Setyo
Published: July 2024

Aims: Patients diagnosed with chronic renal disease commonly experience a diminished quality of life. This systematic review endeavors to discern nursing interventions aimed at enhancing the quality of life among individuals with chronic kidney disease undergoing hemodialysis. These interventions adopt a holistic approach, encompassing psychological, social, and physical aspects.
Methods: A systematic review methodology was employed, utilizing ProQuest, Science Direct, and Google Scholar to search for relevant publications. In order to retrieve pertinent information, the databases were searched for complete English-language research articles published from 2013 to 2023.
Results: This study investigates two intervention categories, specifically physical and non-physical, aimed at improving the quality of life for individuals undergoing hemodialysis due to chronic renal disease. Deliberations reveal that non-physical activities positively impact the psychological and social well-being of chronic kidney disease patients undergoing hemodialysis, whereas physical exercise promotes increased strength and endurance
Conclusions: Dialysis has an adverse effect on all dimensions of Quality of Life for individuals with Chronic Kidney Disease. This review serves as a valuable resource for clinicians, offering insights into the implementation of comprehensive nursing interventions for Chronic Kidney Disease patients undergoing dialysis, encompassing physical, psychological, and social aspects. Subsequent research endeavors should prioritize exploring the spiritual dimension, given the promising outcomes observed in spiritual interventions, which have demonstrated effectiveness in mitigating pain, addressing concerns related to death, and enhancing overall well-being.
Impementasi Enkripsi Data Dokumen pada Kantor Desa Seren Selimbau Menggunakan Algoritma AES
Authors: Nurmala  , P Noviyanti
Published: July 2024

Kantor desa Seren Selimbau memiliki banyak dokumen penting yang perlu dilindungi dari akses yang yang tidak sah. Implementasi dari algoritma AES bertujuan untuk meningkatkan keamanan data dan mencegah kebocoran informasi.Penelitian ini membahas tentang implementasi sistem enkripsi data dokumen di Kantor Desa Seren Selimbau. Algoritma AES dipilih karena memiliki tingkat keamanan yang tinggi dan  telah menjadi standar enkripsi data yang diakui secara internasional. Sistem enkripsi data dokumen ini dikembangkan menggunakan bahasa pemrograman  C#. Sistem ini memungkinkan pengguna untuk mengenkripsi dokumen dengan mudah dan aman.
The Effectiveness of Education with Video in Increasing Family Support and Compliance with Treatment for Hypertension Patients
Authors: Masnah Cek , Suharti Suharti , Heryani Erna , Berliana Novi
Published: July 2024

Hypertension is a national problem in Jambi Province and East Tanjung Jabung Regency. This situation is exacerbated by low regularity of taking medication in East Tanjung Jabung Regency (39.07%), which is far below the national figure of 54.40%. The behavior of taking medication regularly is closely related to a person's adherence to following recommended or prescribed treatment process. In this regard, the study aims to determine effectiveness of video educational media increasing family support and adherence of hypertensive patients to undergo treatment process. Research is quasi-experimental and non-randomized pre-post for control group. The participants were determined by non-probability with 40 people for intervention group and 40 people as controls. The independent sample difference test used Mann-Whitney test and paired sample difference test used Wilcoxon Signed Ranks test. Determine effectiveness of intervention using N-Gain Score test. Results before intervention there was no significant difference between intervention and control groups regarding family support (Asymp. Sig. = 0.853), as well as regard level of adherence (Asymp. Sig. = 0.785). After intervention, there were significant differences regarding family support (Asymp. Sig. = 0.000) and level of adherence (Asymp. Sig. = 0.000). There were significant differences regarding family support and adherence, before and after the intervention, both in intervention and control groups (Asymp. Sig. = 0.000). Video is effective increasing family support (average 47.23%) and compliance (average 53.07%), with moderate effectiveness. The Randomized controlled trials (RCTs), the larger sample sizes, longer follow-up periods, and objectives and outcomes measurements face limitations in the study. Additional future needs to explore complementary intervention or multi-component approaches to optimize hypertension management and patient outcomes
The Holistic Nursing Competencies and Instruments for Critically III-Patients: A Scoping Review
Authors: Ismail Suhartini , Sohilait Hana Priscilla Frudence , Erawati Meira
Published: July 2024

The holistic nursing approach is an extensive model covering all aspects of care, including the patient's psychological, spiritual and social needs. Critically ill patients areas requires a holistic approach because the problems that arise are not just physical problems, but also emotional, mental and cultural in nature. The aim of this study was to determine holistic nursing competencies for critically ill patients and the instruments of holistic competencies from several literatures. The review used Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines as the analysis method. There were 17 articles reviewed from data found in several reputable databases. Searching for related literature, this article uses data based on Science Direct,Scopus, Pro-Quest,Research Gate and SpringerLink.Liiterature search using Booleon operators "OR/AND". The inclusion criteria used in this review include: encompassing holistic nursing, participants being critically ill patients, articles published between 2018-2023, open-access, written in English, and relevant to the research. Articles that did not use English, reports, editorials were excluded.  Dimensions of the holistic nursing competencies domain, namely the physical dimension, including emotional, psychological, social, and spiritual; By treating patients as complex individuals with diverse needs, the holistic approach can help achieve better outcomes in patient recovery and well-being for critical ill patients. The holistic approach can help achieve better outcomes in patient recovery and enhancing the quality of life for the patient., however the holistic nursing is an extensive model covering all aspects of caring.
Influencing Factors on the Postoperative Patient Functional Status at the Meuraxa Regional General Hospital, Banda Aceh
Authors: Ritawati Ritawati , Nurhayati Nurhayati , Baharuddin Baharuddin , Muna Ulfa , Faisal T. Iskandar , Khaira Nuswatul
Published: July 2024

After surgery, the patient will experience pain which can change the role and function in carrying out activities of daily living (ADL), the patient will depend on his family or other people for his life. The aim is to determine the factors that influence the functional status of post-operative patients at the Meuraxa Regional General Hospital, Banda Aceh. Method: using analytical observational methods with a cross-sectional approach. The population in this study were all post-operative care patients at Meuraxa Regional Hospital. The sample in this study were patients treated on the second day after minor surgery at Meuraxa Regional Hospital Banda Aceh with a sample size of 43 respondents. The statistical test uses the chi-square test with a significant level (α=0.05). The research results show that the factors that influence postoperative functional status are pain and fall-efficacy factors (p<0.05), while the age factor is not significantly correlated (p>0.05). Conclusion: Pain and fall-efficacy factors are the most important factors influencing postoperative active status and are statistically significant.
Relationship Between Knowledge, Attitude and Supervision Hand Hygiene Compliance
Authors: Tien Wartini , Komariah Maria , Nurhakim Furkon
Published: July 2024

Health Care Associated Infection has become a serious problem in hospitals, and one of the causes is low hand hygiene compliance. Factors influencing compliance with the five moments of hand hygiene are knowledge, attitudes, and supervision. Research was conducted to identify factors related to nurses' compliance with the implementation of the five moments of hand hygiene. Analytical correlational research with a cross-sectional approach was conducted at RSUD in November 2023 with a sample of 123 nurses. The questionnaire used was based on Herzbergh's motivation theory and the 26-item Manchester Clinical Supervision Scale (MCSS-26). Bivariate test using Chi Square, and multivariate analysis with logistic regression were performed. The results showed that there is a relationship between knowledge and compliance (p=0.000), there is a relationship between training and compliance (p=0.016). There is a relationship between motivation and compliance (p=0.000), there is a relationship between attitudes and compliance (p=0.000). There is a relationship between facilities and compliance (p=0.011), there is a relationship between supervision and compliance (p=0.000). The dominant factors influencing are supervision (3.178), attitude (2.921), and knowledge (0.138). The conclusion of the study is that there is a relationship between knowledge, training, attitudes, motivation, facilities, and supervision on compliance with the five moments of hand hygiene, the dominant factors influencing are supervision, attitude, and knowledge in order.
Role of Rhipicephalus bursa larvae in transstadial transmission and endemicity of Babesia ovis in chronically infected sheep
Authors: Firat Recep , Ulucesme Mehmet Can , Aktas Munir , Ceylan Onur , Sevinc Ferda , Bastos Reginaldo G. , Suarez Carlos E. , Ozubek Sezayi
Published: July 2024

Babesia ovis, transmitted by Rhipicephalus bursa ticks, is the causative agent of ovine babesiosis, a disease characterized by fever, anemia, hemoglobinuria, and high mortality in sheep. This study investigates whether sheep that survived babesiosis without treatment can serve as a source of infection for B. ovis-free host-seeking R. bursa larvae in a later season. Three donor sheep were experimentally infected with B. ovis, and after six months, persistence of B. ovis was assessed through blood and tick transmission experiments. Blood from donor sheep was intravenously injected into three recipient sheep, while donor sheep were also infested with B. ovis-free R. bursa larvae. Engorged nymphs molted to adults, and new recipient sheep were infested with these ticks. All recipient sheep were monitored for B. ovis for 100 days using microscopic, serological, and molecular approaches. The presence of B. ovis was confirmed in the recipient sheep that received blood, leading to clinical infection in two. However, no B. ovis was detected in recipient sheep infested with ticks. These results suggest that sheep recovering from B. ovis infection do not serve as a source of infection for R. bursa larvae in subsequent seasons.
Left bundle branch pacing in third-degree atrioventricular block following morrow surgery: a case report
Authors: Huang Keqiang , Gan Hongmei , Jiang Jingjing , Tang Cheng
Published: July 2024

Left bundle branch pacing (LBBP) has proven to be an alternative method for delivering physiological pacing to achieve electrical synchrony of the left ventricle (LV), especially in patients with atrioventricular block and left bundle branch block (LBBB). However, it is unclear whether it still achieved in patients whose left bundle branch (LBB) has had surgery-induced damage. The Morrow operation (Morrow septal myectomy) is regarded as one of the most effective treatments for hypertrophic obstructive cardiomyopathy (HOCM). The surgery resects small sections of muscle tissue in the proximal ventricular septum nearby or contains the LBB, which means that physical damage to the LBB is almost inevitable. Approximately 2%–12% of patients may need pacemaker implanted after Morrow surgery. LBBP is a feasible and effective method for achieving electric resynchronization of LBBB compared to right ventricular pacing (RVB). Nevertheless, there is a dearth of data on LBBP in third-degree atrioventricular block (AVB) following Morrow surgery. We report a case of successful LBBP in those patients.
Cardioneuroablation: the known and the unknown
Authors: Marrese A. , Persico R. , Parlato E. , Faccenda D. , Salucci A. , Comparone G. , Pergola V. , Ammirati G. , Addeo L. , Fonderico C. , Cocchiara L. , Volpe A. , Visconti P. , Rapacciuolo A. , Strisciuglio T.
Published: July 2024

Cardioneuroablation (CNA) is a novel interventional procedure for the treatment of recurrent vasovagal syncope (VVS) and advanced atrioventricular block secondary to hyperactivation of vagal tone in young patients. By damaging the cardiac parasympathetic ganglia, CNA seems to be able to mitigate and/or abolish the excessive vagal activity and improve patients’ outcome. This review is intended to give a detailed and comprehensive overview of the current evidences regarding (1) the clinical applications of CNA (2) the identification of ablation targets and procedural endpoints (3) the medium-long term effect of the procedure and its future perspectives. However, clinical data are still limited, and expert consensus or recommendations in the guidelines regarding this technique are still lacking.
Enhancing institutional integration and enjoyment among Saudi female physical education students: exploring the mediation of motivation and psychological needs satisfaction
Authors: Frikha Mohamed , Chaâri Nesrine , Mezghanni Nourhen , Hassan Ahmad K. , Alhumaid Majed M. , Alibrahim Mohammed S.
Published: July 2024

Institutional integration and enjoyment in physical education (PE) represent catalysts for greater students’ social cohesion and academic performance. This cross-sectional study addressed the lack of data and examined the mediating effect of basic psychological needs satisfaction (PNS) and motivation on the association between physical activity enjoyment (PAE) and institutional integration (SII) in Saudi female PE students. Participants (N = 507) were invited to complete the PE autonomy relatedness competence scale, the PE motivation scale, the students’ institutional integration scale, and the enjoyment questionnaire. The direct and indirect effects were explored using the SPSS Hayes process macro (Model 4). The results indicate that autonomy, relatedness and extrinsic motivation partially mediate the relationship between the PAE and SII in female Saudi PE students. Therefore, the PAE had a direct effect on SII of 16.2% and an indirect effect of 25.3% through the mediation of autonomy, relatedness, and extrinsic motivation. The psychological processes that promote the development of SII are PAE, autonomy, relatedness, and extrinsic motivation but not intrinsic motivation nor competence. The results are discussed within the self-determination theory and can be used by benefic for students, teachers, administrators or program makers to support institutional integration and enjoyment perception in Saudi female PE students.
Case report: Xeroderma pigmentosum Group A with erythropoietic protoporphyria in a young Chinese patient
Authors: Wu Shu-hui , Xiao Ting , Zhao Dan , Zeng Ying-hong , Zhu Ming-fang
Published: July 2024

Xeroderma pigmentosum is a rare autosomal recessive genodermatoses characterized by a deficiency in nucleotide excision repair. Erythropoietic protoporphyria is a rare inherited metabolic disease caused by the perturbation of heme. Xeroderma pigmentosum-erythropoietic protoporphyria is exceedingly rare. Hereby, we firstly report a young Chinese patient of xeroderma pigmentosum Group A with erythropoietic protoporphyria carrying an XPA Met214AsnfsTer7 frameshift mutation and a homozygous splicing mutation, c.315–48T>C, in the proband’s intron3 of FECH.
Bibliometric analysis of research on China’s rural environmental governance in CNKI and WOS
Authors: Shen Qin , Sun Qing , Zhao Aopei
Published: July 2024

At present, the global consensus on the concept of sustainable development continues to deepen, and China’s ecological civilization construction and rural revitalization strategy continue to advance. However, the rural environmental situation in China is becoming more and more complex. Therefore, a comprehensive review and summary of China’s rural environmental governance research are particularly important, which can not only provide theoretical support for future research but also offer vital guidance for governance practices. Based on the research literature of CNKI (1993–2023) and WOS (2001–2024), bibliometrics and CiteSpace software are adopted in this review to tease out the main characteristics, research hotspots and evolution trends of rural environmental governance research in China. The results indicate that: 1) Research interest shows an overall upward trend, with an expanding scale of interdisciplinary scholars and teams emerging. The collaboration network among authors and institutions in WOS appears tighter than that in CNKI. 2) In CNKI, Research hotspots revolve around environmental issues and pollution governance, social participation and governance mechanisms, ecological civilization and sustainable development, and rural revitalization and construction. In WOS, research hotspots pivot towards policy implementation and governance mechanisms, environmental issues and resource management, socio-economic impacts and equity, information disclosure and community participation, and technology and methods. 3) The research in CNKI has undergone three stages: “initiation”, “development”, and “deepening”. In contrast, the research in WOS started relatively late and is currently still in the “development” stage. Future research should focus on enhancing disciplinary integration and fostering collaboration among domestic and international scholars and institutions. Under the rural revitalization strategy, focus should be on achieving symbiosis between rural economic growth and ecological civilization construction. Simultaneously exploring diverse stakeholder governance models, focusing on rural living environment issues and striving to reduce rural carbon emissions to address climate change challenges.
Spatiotemporal analysis of land-use change and its impact on surface runoff in Tsushima Island, Japan
Authors: Tallar Robby Yussac , Mauregar Golan Geldoffer , Hirose Eishi
Published: July 2024

Previous research on watershed assessments has primarily emphasized general hydrological or technical findings, neglecting the spatiotemporal analysis of land-use change and its influence on surface runoff. This study addresses this gap by using a geographic information system (GIS)-based method to analyze surface runoff variations due to land-use change on Tsushima Island, Japan. By leveraging spatiotemporal analysis within a GIS framework, the study interprets satellite imagery (Landsat 8 data, 2014–2024) to assess land use and watershed characteristics. This approach culminates in a comprehensive map depicting potential flood risk across the island. In conclusion, this study offers an advanced method for communicating potential flood risks to the public and policymakers, enabling proactive mitigation strategies for the coming decade.
Systematic profiling of mitochondria-related transcriptome in tumorigenesis, prognosis, and tumor immune microenvironment of intrahepatic cholangiocarcinoma: a multi-center cohort study
Authors: Chen Bo , Lu Mengmeng , Chen Qiwen , Zou Enguang , Bo Zhiyuan , Li Jiacheng , Zhao Rui , Zhao Jungang , Yu Zhengping , Chen Gang , Wu Lijun
Published: July 2024

Background

Mitochondrial dysfunction has been shown to play a critical role in cancer biology. However, its involvement in intrahepatic cholangiocarcinoma (iCCA) remains significantly understudied.

Methods

RNA sequencing data of 30 pairs of iCCA and paracancerous tissues were collected from the First Affiliated Hospital of Wenzhou Medical University (WMU). The WMU cohort (n = 30) was integrated with public TCGA (n = 30) and GSE107943 (n = 30) datasets to establish a multi-center iCCA cohort. We merged the TCGA and GSE107943 cohorts into an exploration cohort to develop a mitochondria signature for prognosis assessment, and utilized the WMU cohort for external validation. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Hallmarker analyses were used for functional interpretation of iCCA associated mitochondria-related genes (MRGs). In addition, unsupervised clustering was performed to identify mitochondria-based iCCA subtypes with the data of three institutions. Further investigations were conducted to examine the impact of mitochondrial dysfunction on drug responses, alteration of the tumor immune microenvironment, and immune responses.

Results

Two hundred and sixty-three iCCA-related MRGs were identified to be related to fatty acid metabolism, oxidative phosphorylation, and apoptosis. Through univariate and multivariate Cox, and LASSO analyses, a mitochondria signature with five optimal MRGs was established to evaluate the prognosis of iCCA patients with the AUC values ranged from 0.785 to 0.928 in the exploration cohort. The signature also exhibited satisfactory performance in the WMU cohort with AUC values of 0.817–0.871, and was identified as an independent risk predictor in both cohorts. Additionally, we found that patients with higher mitochondria score with poor prognosis presented lower infiltration levels of CD4+ T-cell, NK cells, and monocytes, and demonstrated higher sensitivity to targeted therapies, including sorafenib. Furthermore, two distant mitochondria-based subtypes were determined, and subtype 2 was associated with shorter survival time and immunosuppressive tumor microenvironment. Finally, the differential protein expression of five key MRGs was verified by Immunohistochemistry.

Conclusion

We found mitochondrial dysfunction modulates aberrant metabolism, oxidative stress, immune responses, apoptosis, and drug sensitivity in iCCA. A mitochondria signature and two mitochondria-based iCCA subtypes were identified for clinical risk stratification and immunophenotyping.
4th booster-dose SARS-CoV-2 heterologous and homologous vaccination in rheumatological patients
Authors: Gallardo-Nelson Maria Jose , Cruces Marcos , Gómez Yolanda M. , Fuenzalida Constanza , Silva Javiera , Aravena-Traipi Laura , Nuñez Eduardo , Gaete-Angel Aracelly , Rivas-Yañez Elizabeth , Kalergis Alexis M. , Soto-Rifo Ricardo , Valiente-Echeverria Fernando
Published: July 2024

Objective

to evaluate the immune response to the SARS-CoV-2 vaccines in adults with immune-mediated rheumatic diseases (IMRDs) in comparison to healthy individuals, observed 1-20 weeks following the fourth vaccine dose. Additionally, to evaluate the impact of immunosuppressive therapies, vaccination schedules, the time interval between vaccination and sample collection on the vaccine’s immune response.

Methods

We designed a longitudinal observational study conducted at the rheumatology department of Hospital de Copiapó. Neutralizing antibodies (Nabs) titers against the Wuhan and Omicron variant were analyzed between 1-20 weeks after administration of the fourth dose of the SARS-CoV-2 vaccine to 341 participants (218 IMRD patients and 123 healthy controls). 218 IMRD patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), ankylosing spondylitis (AS), systemic lupus erythematosus (SLE), systemic vasculitis (VS) and systemic scleroderma (SS) were analyzed.

Results

Performing a comparison between the variants, Wuhan vs Omicron, we noticed that there were significant differences (p<0.05) in the level of the ID50, both for healthy controls and for patients with IMRDs. The humoral response of patients with IMRDs is significantly lower compared to healthy controls for the Omicron variant of SARS-CoV-2 (p = 0.0015). The humoral response of patients with IMRDs decreases significantly when the time interval between vaccination and sample collection is greater than 35 days. This difference was observed in the response, both for the Wuhan variant and for the Omicron variant.

Conclusion

The IMRDs patients, the humoral response variation in the SARS-CoV-2 vaccine depends on doses and type of vaccine administered, the humoral response times and the treatment that these patients are receiving.
Unraveling the role of NLRP3 inflammasome in allergic inflammation: implications for novel therapies
Authors: Lu Hui-Fei , Zhou Yi-Chi , Hu Tian-Yong , Yang Dun-Hui , Wang Xi-Jia , Luo Dan-Dan , Qiu Shu-Qi , Cheng Bao-Hui , Zeng Xian-Hai
Published: July 2024

Allergic diseases like asthma, allergic rhinitis and dermatitis pose a significant global health burden, driving the search for novel therapies. The NLRP3 inflammasome, a key component of the innate immune system, is implicated in various inflammatory diseases. Upon exposure to allergens, NLRP3 undergoes a two-step activation process (priming and assembly) to form active inflammasomes. These inflammasomes trigger caspase-1 activation, leading to the cleavage of pro-inflammatory cytokines (IL-1β and IL-18) and GSDMD. This process induces pyroptosis and amplifies inflammation. Recent studies in humans and mice strongly suggest a link between the NLRP3 inflammasome, IL-1β, and IL-18, and the development of allergic diseases. However, further research is needed to fully understand NLRP3’s specific mechanisms in allergies. This review aims to summarize the latest advances in NLRP3 activation and regulation. We will discuss small molecule drugs and natural products targeting NLRP3 as potential therapeutic strategies for allergic diseases.
Efficacy of calcein as a chemical marker of Potamocorbula laevis
Authors: Gao Jin , Xie Xi , Liu Xiang-Feng , Bai Yong-An , Yang Miao , Teng Wei-Ming , Liu Hai-Jiao , Wang Qing-Zhi
Published: July 2024

Introduction

Calcein was used to develop a shell marking method for Potamocorbula laevis.

Methods

The suitable conditions for marking were investigated, including marking concentration, immersion time, and water temperature. The impacts and feasibility of the marking method were assessed based on the survival rate of P. laevis, the success rate of fluorescence marking, marking quality, and alterations in activities of antioxidant enzymes in the digestive gland of the experimental bivalves. Two concentrations of calcein (20 and 50 mg/L) were used and the immersion time included 1 and 2 h, respectively. The experiment was performed in two rounds, with water temperatures of 12.84 ± 0.09 and 24.18 ± 0.04 °C, respectively.

Results and discussion

The results indicated that calcein did not significantly impact the survival of P. laevis after 7 d of recovery. The catalase activity and malondialdehyde content in low temperature-marked P. laevis showed significant decreases, and the relative abundances of certain fatty acids also exhibited significant changes within 2 h post exposure to 20 mg/L of calcein. However, these indicators returned to normal levels within 7 d. The marking impact of calcein was proportional to the calcein concentration and immersion time. Higher temperature generated a negative impact on the marking effect of 20 mg/L of calcein, while no obvious impacts were observed for 50 mg/L of calcein. The marking success rates and the recapture rates of P. laevis for in situ tests in the two experimental groups were both 100% and 4.44 ± 1.29% after one month. Also, the recapture marking rates and the marking good rates of the recaptured individuals were both 100%. There were no significant differences between these parameters for 50 and 75 mg/L of calcein. Given the cost and safety of labeling, a strategy in terms of an immersion in 50 mg/L of calcein for 2 h could be considered as an effective in situ labeling scheme for P. laevis. In conclusion, calcein can be employed as a marking method for P. laevis. These findings could be potentially beneficial for development of in situ labeling technology, proliferation as well as release of shellfish in tidal flats and resource conservation.
Benthic metabolism and nutrient dynamics of a hyperturbid and hypernutrified estuary
Authors: Perez-Rodriguez Valle , Corzo Alfonso , Papaspyrou Sokratis , van Bergeijk Stefanie Anne , Vilas Cesar , Cañavate José Pedro , Garcia-Robledo Emilio
Published: July 2024

The biogeochemical role of the sediments in the Guadalquivir River estuary, a vital region in the SW Iberian Peninsula, has been considerably neglected. The benthic microalgae (microphytobenthos, MPB) inhabiting the sediment surface could contribute to the autochthonous primary production and influence nutrient recycling in this hyper-turbid and hypernutrified estuary. Sediment–water column fluxes of O2 and dissolved inorganic nitrogen species (DIN = NH4+ + NO3− + NO2−) were assessed in laboratory incubations of sediment cores from Bonanza (mouth) and Lebrija (middle) during a 1-year study. Vertical profiles down to the 10-cm depth of photosynthetic pigments, organic C, total nitrogen, DIN, Fe2+, SO42−, and dissolved inorganic carbon (DIC) were also measured. Chlorophyll a in the sediment surface was higher at the estuarine mouth, exhibiting a seasonal pattern at both sites with highest values in winter and lowest in summer. Net community production (NCP) was higher in Bonanza compared with Lebrija and showed positive values most of the year, indicating that MPB contributed to the overall autochthonous primary production of the estuary. Seasonal changes in chlorophyll a and NCP were not parallel, suggesting different ecological controls. The sediment was generally a net sink of NO3− and NH4+ in both sites, with several fold higher rates for NO3− uptake. MPB N demand could account for the entire sediment DIN uptake in Bonanza and 21% in Lebrija. The remaining high NO3− uptake rates indicate that they sustain elevated sediment denitrification rates. In contrast, rates of anaerobic oxidation of organic matter by Fe reduction and SO42− reduction, estimated from Fe2+ and SO42− vertical concentration profiles, were several orders of magnitude lower than the estimated water column-dependent denitrification rates. Overall, this study shows the importance of MPB in the Guadalquivir Estuary and the potential dominant role of denitrification in the anaerobic mineralization of organic matter.
Global citrus root microbiota unravels assembly cues and core members
Authors: Lombardo Monia F. , Zhang Yunzeng , Xu Jin , Trivedi Pankaj , Zhang Pengfan , Riera Nadia , Li Lei , Wang Yayu , Liu Xin , Fan Guangyi , Tang Jiliang , Coletta-Filho Helvécio D. , Cubero Jaime , Deng Xiaoling , Ancona Veronica , Lu Zhanjun , Zhong Balian , Roper M. Caroline , Capote Nieves , Catara Vittoria , Pietersen Gerhard , Al-Sadi Abdullah M. , Xu Xun , Wang Jian , Yang Huanming , Jin Tao , Cirvilleri Gabriella , Wang Nian
Published: July 2024

Introduction

Citrus is one of the most important fruit crops worldwide, and the root-associated microbiota can have a profound impact on tree health and growth.

Methods

In a collaborative effort, the International Citrus Microbiome Consortium investigated the global citrus root microbiota with samples collected from nine citrus-producing countries across six continents. We analyzed 16S rDNA and ITS2 amplicon sequencing data to identify predominant prokaryotic and fungal taxa in citrus root samples. Comparative analyses were conducted between root-associated microbial communities and those from the corresponding rhizosphere and bulk soil samples. Additionally, genotype-based group-wise comparisons were performed to assess the impact of citrus genotype on root microbiota composition.

Results

Ten predominant prokaryotic phyla, containing nine bacterial phyla including Proteobacteria, Actinobacteria, Acidobacteria, and Bacteroidetes and one archaeal phylum (Thaumarchaeota), and multiple fungal phyla including Ascomycota and Basidiomycota were identified in the citrus root samples. Compared with the microbial communities from the corresponding rhizosphere and bulk soil samples from the same trees, the prokaryotic and fungal communities in the roots exhibited lower diversity and complexity but greater modularity compared to those in the rhizosphere. In total, 30 root-enriched and 150 root-depleted genera in bacterial community were identified, whereas 21 fungal genera were enriched, and 147 fungal genera were depleted in the root niche compared with the rhizosphere. The citrus genotype significantly affected the root prokaryotic and fungal communities. In addition, we have identified the core root prokaryotic genera comprising Acidibacter, Allorhizobium, Bradyrhizobium, Chitinophaga, Cupriavidus, Devosia, Dongia, Niastella, Pseudomonas, Sphingobium, Steroidobacter and Streptomyces, and the core fungal genera including Acrocalymma, Cladosporium, Fusarium, Gibberella, Mortierella, Neocosmospora and Volutella. The potential functions of these core genera of root microbiota were predicted.

Conclusion

Overall, this study provides new insights into the assembly of microbial communities and identifies core members of citrus root microbiota across a wide geographic range. The findings offer valuable information for manipulating root microbiota to enhance plant growth and health.
Effects of repetitive transcranial magnetic stimulation on lower extremity motor function and optimal parameters in stroke patients with different stages of stroke: a systematic evaluation and meta-analysis
Authors: Fan Shiyu , Yan Long , Zhang Junfeng , Qian Yulin , Wang Meng , Yang Lingqing , Yu Tao
Published: July 2024

Background

Repetitive transcranial magnetic stimulation (rTMS), as an emerging non-invasive neuromodulation technique, is now widely employed in rehabilitation therapy. The purpose of this paper is to comprehensively summarize existing evidence regarding rTMS intervention for lower limb motor function in patients at different stages of stroke.

Methods

A systematic search was conducted to identify randomized controlled trials (RCTs) assessing the efficacy of rTMS for treating lower limb motor dysfunction after stroke. Multiple databases, including China National Knowledge Infrastructure (CNKI), Wanfang Data Knowledge Service Platform, VIP Database, PubMed, Embase, Web of Science, and Cochrane Library, were searched. The search period extended from the inception of the libraries to June 2024. Literature information was extracted, and methodological quality was evaluated using the risk of bias assessment tool in the Cochrane Handbook. Meta-analysis was performed using Stata 17.0 software.

Results

Overall, 49 appropriate studies (including 3,558 stroke subjects) were found. Meta-analysis results demonstrated that rTMS effectively improved lower limb motor function across all stages of stroke. The intervention was particularly more effective in patients in the subacute stage than in the acute or chronic stages. Subgroup analysis revealed that, for acute-stage patients, low-frequency stimulation targeting the M1 or DLPFC brain regions on the unaffected side with 20–40 sessions significantly improved FMA-LE scores. In subacute-phase patients, low-frequency stimulation targeting the M1 brain regions on the unaffected side with 18 sessions significantly improved FMA-LE scores. The results demonstrated that HF-rTMS was more effective than LF-rTMS in improving walking speed, with the greatest efficacy observed at 20 sessions. While for enhancing gait balance in stroke patients, LF-rTMS with the best therapeutic effect was observed at a frequency of 20–40 treatments.

Conclusion

This study demonstrates the efficacy of rTMS in improving lower limb motor function, balance, and walking speed in stroke patients at various stages. The findings provide a valuable reference for the development of optimized rTMS treatment plans in clinical practice.

Systematic review registration: PROSPERO: CRD42023466094.