bluuebunny/crossref_metadata_2025 huggingface dataset:
Die kulturelle Norm der Kernfamilie – Habitusrekonstruktionen und Deutungsmusteranalyse
Authors: Funcke Dorett
Published: December 2019

In diesem Beitrag geht es um eine unkonventionelle Familienform, die gleichgeschlechtliche Familie, und die Frage: Welche sozialisatorischen Voraussetzungen führen dazu, dass trotz Geltung der Norm der Kernfamilie, die im ausgewählten Fall sich im Vollzug von Deutungen empirisch manifestiert, von dieser Regelstruktur abgewichen wird. Die Abweichung zeigt sich in der Wahl einer anonymen Samenspende und darin, dass die Nachwuchssozialisation vom Muster der Kernfamilie abgetrennt ist. Grundlage der Fallrekonstruktionsanalyse sind das Genogramm und eine ausgewählte Sequenzstelle aus dem Interview. Der Beitrag schließt mit allgemeinen Überlegungen zur sozialen Wirklichkeit von alternativen Familien und mit familientheoretischen Reflexionen.
Erziehung unter Druck
Authors: Wolf Eike
Published: December 2019

Der Beitrag befasst sich mit der Interdependenz des dualistischen Ordnungsschemas von Privatheit und Öffentlichkeit der Erziehung aus mikrologischer Perspektive. Mithilfe der interaktionstheoretischen Terminologie Goffmans wird der Einfluss öffentlicher Interaktionsstrukturen auf privates Erziehungshandeln verdeutlicht und mittels der objektiv hermeneutischen Rekonstruktion eines entsprechenden Falls empirisch abgesichert. Die begrifflich-dualistisch operierenden Ordnungskategorien von öffentlicher und privater Erziehung werden so als klassifikatorische Idealtypik zur Handlungsbeschreibung gefasst, die sich empirisch erst anhand geltender Interaktionsordnungen relationieren lassen. Als zentrales Strukturproblem für unter den Bedingungen von Öffentlichkeit sich vollziehende Erziehungspraxis wird die triadische Interaktions-struktur zwischen Erziehungs-Dyade und Öffentlichkeit gefasst.
Positionalität zwischen Sein und Noch-nicht-Sein
Authors: Rademacher Sandra , Tressat Michael
Published: December 2019

Ausgehend von der Frage, was es bedeutet, als Kind und Jugendlicher in einem spezifischen Sozial- und Kulturraum positioniert zu sein und sich zugleich von eben dieser Positionalität ausgehend als (kindliches und jugendliches) Subjekt entwerfen und positionieren zu müssen, analysieren wir in diesem Beitrag aus entwicklungs- und sozialisationstheoretischer Perspektive fotografische Selbstporträts eines neunjährigen Mädchens aus Deutschland und eines dreizehnjährigen Jungen aus Ghana. Als heuristischen Rahmen für „Kindheit“ und „Jugend“ entwerfen wir das Konzept eines dialektischen Spannungsverhältnisses zwischen Sein und Noch-nicht-Sein, das wir als konstitutiv für die Positionalität von Kindern und Jugendlichen erachten. Bei der Analyse betrachten wir daher besonders die Art und Weise, wie sich Positionalität und Position des Fotografierenden in einem fotografischen Protokoll niederschlagen. Die beiden Fallanalysen zeigen, dass dieses Spannungsverhältnis insbesondere im Übergang von der Kindheit zur Jugend thematisch ist und dass Spiegel-Selfies dieses Spannungsverhältnis eines „Zwischenraumes“ geradezu idealtypisch zum Ausdruck bringen.
The location of classified edges due to the change in the geometric multiplicity of an eigenvalue in a tree
Authors: Toyonaga Kenji
Published: December 2019

Given a combinatorially symmetric matrix A whose graph is a tree T and its eigenvalues, edges in T can be classified in four categories, based upon the change in geometric multiplicity of a particular eigenvalue, when the edge is removed. We investigate a necessary and sufficient condition for each classification of edges. We have similar results as the case for real symmetric matrices whose graph is a tree. We show that a g-2-Parter edge, a g-Parter edge and a g-downer edge are located separately from each other in a tree, and there is a g-neutral edge between them. Furthermore, we show that the distance between a g-downer edge and a g-2-Parter edge or a g-Parter edge is at least 2 in a tree. Lastly we give a combinatorially symmetric matrix whose graph contains all types of edges.
Numerical construction of structured matrices with given eigenvalues
Authors: Sutton Brian D.
Published: December 2019

We consider a structured inverse eigenvalue problem in which the eigenvalues of a real symmetric matrix are specified and selected entries may be constrained to take specific numerical values or to be nonzero. This includes the problem of specifying the graph of the matrix, which is determined by the locations of zero and nonzero entries. In this article, we develop a numerical method for constructing a solution to the structured inverse eigenvalue problem. The problem is recast as a constrained optimization problem over the orthogonal manifold, and a numerical optimization routine seeks its solution.
Extensions of the Eneström-Kakeya theorem for matrix polynomials
Authors: Melman A.
Published: December 2019

The classical Eneström-Kakeya theorem establishes explicit upper and lower bounds on the zeros of a polynomial with positive coefficients and has been generalized for positive definite matrix polynomials by several authors. Recently, extensions that improve the (scalar) Eneström-Kakeya theorem were obtained with a transparent and unified approach using just a few tools. Here, the same tools are used to generalize these extensions to positive definite matrix polynomials, while at the same time generalizing the tools themselves. In the process, a framework is developed that can naturally generate additional similar results.
Martensitic Steel Microstructure Effects on Cavitation Erosion
Authors: Ylönen Markku , Nyyssönen Tuomo , Honkanen Mari , Peura Pasi
Published: December 2019

Cavitation and cavitation erosion are sometimes unavoidable phenomena in hydro machine operation. For example, operating hydro turbines as regulating power leads to situations in which the risk of cavitation is accepted to some extent. Cavitation-resistant materials are therefore required to reduce machine damage and maintenance. This study characterizes the microstructure of two martensitic stainless steels from Francis turbines. They were already studied for cavitation erosion resistance in a previous study, and this study reveals the reasons behind the other steel having a significantly better resistance, while they had similar chemical compositions. The electron backscatter diffraction (EBSD) method is effective in defining the martensitic microstructure, specifically the block, packet, and prior austenite grain level. In addition, the retained austenite is effectively detected with the method. A fine prior austenite grain size and small packet and block sizes were found to be among the defining factors in cavitation erosion resistance of these steels. In addition, the transformation of retained austenite to martensite was detected in the edge region where cavitation had taken place. This transformation further increases cavitation erosion resistance. The better resistance of Steel 1 against cavitation was attributed to these microstructural differences. According to these findings, the microstructure in Steel 1 would be highly beneficial in building cavitation erosion resistant hydro machines and would be of interest to manufacturers of martensitic stainless steel components.
Genetic Control and Geo-Climate Adaptation of Pod Dehiscence Provide Novel Insights into Soybean Domestication
Authors: Zhang Jiaoping , Singh Asheesh K
Published: December 2019

Loss of pod dehiscence was a key step in soybean [Glycine max (L.) Merr.] domestication. Genome-wide association analysis for soybean shattering identified loci harboring Pdh1, NST1A and SHAT1-5. Pairwise epistatic interactions were observed, and the dehiscent Pdh1 overcomes resistance conferred by NST1A or SHAT1-5 locus. Further candidate gene association analysis identified a nonsense mutation in NST1A associated with pod dehiscence. Geographic analysis showed that in Northeast China (NEC), indehiscence at both Pdh1 and NST1A were required in cultivated soybean, while indehiscent Pdh1 alone is capable of preventing shattering in Huang-Huai-Hai (HHH) valleys. Indehiscent Pdh1 allele was only identified in wild soybean (Glycine soja L.) accession from HHH valleys suggesting that it may have originated in this region. No specific indehiscence was required in Southern China. Geo-climatic investigation revealed strong correlation between relative humidity and frequency of indehiscent Pdh1 across China. This study demonstrates that epistatic interaction between Pdh1 and NST1A fulfills a pivotal role in determining the level of resistance against pod dehiscence, and humidity shapes the distribution of indehiscent alleles. Our results give further evidence to the hypothesis that HHH valleys was at least one of the origin centers of cultivated soybean.
Karakteristik Perusahaan Terhadap Luas Pengungkapan Corporate Social Responsibility
Authors: Prakoso Aryo , Dewinta Aulia Ayu
Published: December 2019

Globalization provides open competition to every business actor, to become a superior and valuable company one of the ways that can be taken by the company through disclosure of the corporate social reports (CSR). This paper wants to explore how far the characteristics of the company influence the extent of CSR disclosure. The method in this study uses the empirical study hypothesis testing approach by sampling all companies indexed L-Q45 on the Indonesia stock exchange. The reason selection of this index is the leading index in Indonesia with tightly defined criteria by regulators, including levels of liquidity, market capitalization, and growth prospects of the company. The results of the data analysis that has been done obtained results Profitability and total assets influence the broad disclosure of CSR, this also supports stakeholder theory and legitimacy theory, on the contrary, leverage does not affect the broad disclosure of CSR. That caused by the management of the company seeing high leverage tends to reduce the disclosure of social responsibility so as not to be in the spotlight of the debtholders
Does Transfer Motivation Succeed in Mediating in the Context of Training Transfer?
Authors: Wirdani Dida Sovia , Wulansari Nury Ariani
Published: December 2019

The purpose of this study is to examine and find out the mediating effect of transfer motivation on the relationship between supervisor support and peer support on training transfers in Kantor Pelayanan Pajak (KPP) Pratama Semarang Timur and Selatan employees. This study took a sample of 130 employees by using proportional random sampling technique. The data collection method was carried out by means of a survey, namely interviews and questionnaires. The data analysis method uses descriptive statistical tests and partial least square. The results of this study were accepted both direct and indirect effects (mediation). The conclusion of this study is that KPP Pratama Semarang Timur and Selatan employees must improve the implementation of training results to work and believe that the successful implementation of knowledge, attitude skills can improve their performance.
Pengaruh Perencanaan Pajak dan Profitabilitas Terhadap Nilai Perusahaan dengan BOD Diversity sebagai Variabel Moderasi
Authors: Astuti Yuli Dwi , Fitria Giawan Nur
Published: December 2019

This study aims to determine the effect of tax planning and profitability on firm value with BOD diversity as a moderating variable. This research is motivated by the importance of information about the factors that can affect the value of the company. Company value in this study used price book value as an indicator of measurement. The population of this research is the property and real estate sector companies in companies listed on the Indonesia Stock Exchange in 2016 - 2018. The sample of this research is 30 issuers or 90 company financial statement data used in this study. This study uses multiple linear regressiosn with statistical tests and moderate regression analysis test with SPSS 23. The results of this study are tax planning and profitability have a positive and significant effect on firm value. Whereas BOD Diversity weakens the relationship between tax planning and profitability on firm value.
Reaksi Pasar terhadap Pengumuman Penawaran Saham Terbatas di Indonesia
Authors: Sutrisno Bambang , Priharta Andry
Published: December 2019

The right issue announcement may lead the market to react and affect the stock performance. This study aims to analyze the effect of the right issue on the abnormal return. This research uses a sample of 18 firms listed in the Indonesia Stock Exchange from 2014 to 2016. This study employs one sample t test and wilcoxon test. The results show that there is no abnormal return in the days surrounding right issue announcement. The results also find that there is no difference in abnormal return between after and before the right issue announcement. This research implies that the existing stockholders and investors need to consider carefully before deciding to invest in go public companies do the right issue.
The Effect of Augmented Reality Shopping Applications on Purchase Intention
Authors: Leonnard Leonnard , Paramita Annisa S. , Maulidiani Jasmine J.
Published: December 2019

Augmented reality is a new technological breakthrough that helps e-commerce delivers an online shopping experience with quality of offline shopping. This is possible due to the capability of augmented reality technology that allows consumers to interact and to try products through the virtual world. This technology has not been widely adopted by e-commerce in Indonesia. In this study, we analyzed the effect of this technology on purchase intention through e-consumer experience in the form of perceived enjoyment and usefulness to 89 consumers. Our findings revealed that virtual presence significantly affects the enjoyment and usefulness directly and indirectly to purchase intention. In addition, the device also significantly affects the enjoyment and usefulness directly. Finally, the usefulness is proven to significantly affect purchase intention.
The effect of sports shoes on flat foot
Authors: Korkmaz M.F. , Acak M. , Duz S.
Published: December 2019

Objective: This study was conducted to determine the effects of participation in sports on the structural deformities of the foot. Methods: A total of 2013 athletes (1301 males, 712 females) who had regularly participated in sports for a minimum of three years were included in the study. Screening of the sole was performed using a podoscope as it is a reliable and practical device. The structure of the sole was evaluated with the highly valid Staheli index. Results: A significant relationship was detected between the ages of the athletes, years in sport, branch of sports variables and the prevalence of flat foot. However, no significant relationship was found between the athletes’ gender and the prevalence of flat foot. Conclusion: It is thought that the training regimes, the floor on which the sports is performed and the shoes used in sports had effects on flat foot deformity. Based on our results, we assert that redesigning the competition shoes and using sports shoes with arch support during warm-ups, running and other sportive and educational drills outside the routine activities of the sports branch may be beneficial for foot health.
Physical condition of pupils of pre-school educational establishments of different types
Authors: Moskalenko N.V. , Savchenko V.G. , Polyakova A.V. , Mikitchik O.S. , Mitova O.O. , Griukova V.V. , Mytsak A.V.
Published: December 2019

The purpose of the study is to determine the physical condition of the pupils of pre-school educational establishments of various types for improving the quality of the educational process of physical education. Material and methods . The study involved 3-4 year-old children. The children attended preschool institutions of various types in the city of Dnipro. They were the children's educational establishment №282 of the combined type, the children's educational establishment №192 of the sanatorium type, the children's educational establishment №28 of the compensating type, children's educational establishment №244 and №404 of general development. Control (n = 90) and experimental (n = 95) groups were created in each age group of a particular type of institution. The differentiation of pupils of institutions of different types into two subgroups was caused by their differences in functional indicators and health status. At the beginning of the experiment, the homogeneity of the studied groups of children was noted (p> 0.05). Results. The model of organization of rational motor mode of 3-4 year-old children has been substantiated and developed. Organizational and methodological bases of realization of the model of rational motor mode of 3-4 year-old children in preschool educational institutions of various types have been developed. The influence of the developed model on the physical condition of pupils of pre-school educational institutions of different types has been determined. The study of the anthropometric parameters of children after the implementation of the developed model showed no differences in these parameters. These differences were observed in children of different types of kindergartens in all sex-age groups. The determination of functional abilities of children resulted in a significant improvement in spirometry, respiratory rate, heart rate, Ruffier test in experimental groups of children of both sexes (p <0.05). The indicators of the functional state of the cardiovascular system and physical performance of the pupils of preschool educational establishments of the sanatorium type and the compensating type were lower than the similar indicators of the pupils of general development establishments. The indicators of heart rate of real rest and respiratory rate of children of experimental groups of institutions of the sanatorium and compensating type corresponded to the average level. Conclusions. The obtained result of this empirical study confirms the hypothesis that the organization of rational movement mode for 3-4 year-old children contributes to improving of their physical condition. The obtained results indicate the improvement of the quality of the educational process of physical education of children in pre-school educational establishments of different types.
O tutor como agente facilitador no processo de ensino e aprendizagem: uma experiência na disciplina de Bioquímica Metabólica
Authors: Bernardo Lucas Coêlho , Silva Nathália Regina Galvão , Freitas Júnior Augusto Cézar Vasconcelos , Felipe Cícero Francisco Bezerra , Farias Davi Felipe
Published: December 2019

A tutoria faz parte dos programas acadêmicos oferecidos pela Universidade Federal da Paraíba (UFPB), com objetivo de auxiliar os discentes a partir da implementação de estratégias que facilitem o percurso dos estudantes pelas disciplinas com maiores índices de reprovação e evasão. Este trabalho teve como objetivo avaliar o papel do tutor como agente no processo de ensino e aprendizagem na disciplina de Bioquímica Metabólica, na UFPB. Para tanto, os tutores dessa disciplina aplicaram quatro estudos dirigidos e um jogo didático nas turmas dos períodos letivos 2016.2 (Grupo 1/n= 27) e 2017.1 (Grupo 2/n= 23). O desempenho dos discentes que usufruíram dos recursos didáticos foi satisfatório e significativamente maior do que o grupo que não participou. A partir da avaliação da tutoria pelos discentes por meio de um questionário online, respondeu-se unanimemente que a disciplina de Bioquímica Metabólica precisa ofertar tutores, declarando que os mesmos são facilitadores importantes no processo de ensino e aprendizagem.
THE USE OF EDUCATIONAL GAMES TO EXPLAIN COMPLEX CONCEPTS RELATED TO HUMAN PHYSIOLOGY
Authors: Neves Ben-Hur Souto , Machado Rui Seabra , Soare Caroline Bitencourt , Mello-Carpes Pâmela Billig
Published: December 2019

Diferentes estratégias têm sido propostas para melhorar o processo de ensino-aprendizagem em diferentes compos. Uma opção para otimizar o aprendizado, aumentando o envolvimento dos alunos e proporcionando discussões em grupo, é o uso de jogos educacionais (JE). Neste estudo, nós avaliamos as percepções dos alunos sobre o uso do JE como uma ferramenta para facilitar o entendimento de conceitos-chave relacionados à Fisiologia Humana (FH). Jogos abordando os potenciais bioelétricos da membrana celular e o ciclo cardíaco foram utilizados para complementar as aulas teóricas de Fisiologia. Após o uso dos jogos, os alunos foram convidados a avaliá-los. Verificamos que os alunos consideram o JE ferramentas interessantes para melhorar o ensino da FH e que contribuem para a compreensão do conteúdo trabalhado em sala de aula.
O tutor como agente facilitador no processo de ensino e aprendizagem: uma experiência na disciplina de Bioquímica Metabólica
Authors: Farias Davi Felipe
Published: December 2019

A tutoria faz parte dos programas acadêmicos oferecidos pela Universidade Federal da Paraíba (UFPB), com objetivo de auxiliar os discentes a partir da implementação de estratégias que facilitem o percurso dos estudantes pelas disciplinas com maiores índices de reprovação e evasão. Este trabalho teve como objetivo avaliar o papel do tutor como agente no processo de ensino e aprendizagem na disciplina de Bioquímica Metabólica, na UFPB. Para tanto, os tutores dessa disciplina aplicaram quatro estudos dirigidos e um jogo didático nas turmas dos períodos letivos 2016.2 (Grupo 1/n= 27) e 2017.1 (Grupo 2/n= 23). O desempenho dos discentes que usufruíram dos recursos didáticos foi satisfatório e significativamente maior do que o grupo que não participou. A partir da avaliação da tutoria pelos discentes por meio de um questionário online, respondeu-se unanimemente que a disciplina de Bioquímica Metabólica precisa ofertar tutores, declarando que os mesmos são facilitadores importantes no processo de ensino e aprendizagem.
Editorial - 2
Authors: Bianco André Amaral Gonçalves
Published: December 2019

Editorial da edição 2019, volume2.
RESEARCH OF FACTORS INFLUENCING THE PROCESS OF FORMATION OF WELDED MICROCONNECTIONS IN ELECTRONIC MODULES
Authors: NEVLİUDOV İgor , OMAROV Murad , BOTSMAN İryna , DEMSKA Nataliia , NEVLİUDOVA Viktoriia , STARODUBCEV Mykola
Published: December 2019

The features of the formation of microconnections in electronic modules
on an aluminum-polyimide basis by the method of ultrasonic welding, which
allows to obtain reliable mounting joints of different thickness aluminum
without preliminary removal of oxide films where the use of contact welding and
soldering are practically difficult or impossible, are investigated.  The influence of technological modes (power
of an ultrasonic generator, oscillation amplitude of a welding tip (tool),
welding force and welding time) on the reliability of mounting microconnections
is considered
DYNAMICAL FRACTAL ANALYSIS OF THE ACOUSTIC ULTRA-WIDEBAND SIGNAL CAUSED BY THE CHELYABINSK METEOROID
Authors: CHERNOGOR Leonid , LAZORENKO Oleg , ONİSHCHENKO Andrey
Published: December 2019

The non-linear paradigm clams, that many processes in open, non-linear, dynamical systems, which caused by the non-stationary, powerful sources, are appeared to be short-time, non-linear, ultra-wideband and fractal ones. The acoustic signals created by the Chelyabinsk meteoroid fall were shown to be namely ones of them. Using a new fractal analysis method called as ‘Dynamical Fractal Analysis’, the fractal properties of these signals were investigated. The corresponding numerical characteristics were estimated and discussed.
GTP-binding properties of the membrane-bound form of porcine liver annexin VI.
Authors: Kirilenko A , Golczak M , Pikuła S , Bandorowicz-Pikuła J
Published: December 2019

Annexin VI (AnxVI) of molecular mass 68-70 kDa belongs to a multigenic family of ubiquitous Ca2+- and phospholipid-binding proteins. In this report, we describe the GTP-binding properties of porcine liver AnxVI, determined with a fluorescent GTP analogue, 2'-(or 3')-O-(2,4,6-trinitrophenyl)guanosine 5'-triphosphate (TNP-GTP). The optimal binding of TNP-GTP to AnxVI was observed in the presence of Ca2+ and asolectin liposomes, as evidenced by a 5.5-fold increase of TNP-GTP fluorescence and a concomitant blue shift (by 17 nm) of its maximal emission wavelength. Titration of AnxVI with TNP-GTP resulted in the determination of the dissociation constant (Kd) and binding stoichiometry that amounted to 1.3 microM and 1:1 TNP-GTP/AnxVI, mole/mole, respectively. In addition, the intrinsic fluorescence of the membrane-bound form of AnxVI was quenched by TNP-GTP and this was accompanied by fluorescence resonance energy transfer (FRET) from AnxVI Trp residues to TNP-GTP. This indicates that the GTP-binding site within the AnxVI molecule is probably located in the vicinity of a Trp-containing domain of the protein. By controlled proteolysis of human recombinant AnxVI, followed by purification of the proteolytic fragments by affinity chromatography on GTP-agarose, we isolated a 35 kDa fragment corresponding to the N-terminal half of AnxVI containing Trp192. On the basis of these results, we suggest that AnxVI is a GTP-binding protein and the binding of the nucleotide may have a regulatory impact on the interaction of annexin with membranes, e.g. formation of ion channels by the protein.
Sequence analysis of enzymes with asparaginase activity.
Authors: Borek D , Jaskólski M
Published: December 2019

Asparaginases catalyze the hydrolysis of asparagine to aspartic acid and ammonia. Enzymes with asparaginase activity play an important role both in the metabolism of all living organisms as well as in pharmacology. The main goal of this paper is to attempt a classification of all known enzymes with asparaginase activity, based on their amino acid sequences. Some possible phylogenetic consequences are also discussed using dendrograms and structural information derived from crystallographic studies.
Protein phosphatase 2A: variety of forms and diversity of functions.
Authors: Lechward K , Awotunde O S , Swiatek W , Muszyńska G
Published: December 2019

Protein phosphatase 2A (PP2A) comprises a diverse family of phosphoserine- and phosphothreonine-specific phosphatases present in all eukaryotic cells. All forms of PP2A contain a catalytic subunit (PP2Ac) which forms a stable complex with the structural subunit PR65/A. The heterodimer PP2Ac-PR65/A associates with regulatory proteins, termed variable subunits, in order to form trimeric holoenzymes attributed with distinct substrate specificity and targeted to different subcellular compartments. PP2Ac activity can be modulated by reversible phosphorylation on Tyr307 and methylation on C-terminal Leu309. Studies on PP2A have shown that this enzyme may be implicated in the regulation of metabolism, transcription, RNA splicing, translation, differentiation, cell cycle, oncogenic transformation and signal transduction.
Understanding the evolution of restriction-modification systems: clues from sequence and structure comparisons.
Authors: Bujnicki J M
Published: December 2019

Restriction-modification (RM) systems comprise two opposing enzymatic activities: a restriction endonuclease, that targets specific DNA sequences and performs endonucleolytic cleavage, and a modification methyltransferase that renders these sequences resistant to cleavage. Studies on molecular genetics and biochemistry of RM systems have been carried out over the past four decades, laying foundations for modern molecular biology and providing important models for mechanisms of highly specific protein-DNA interactions. Although the number of known, relevant sequences 3D structures of RM proteins is growing steadily, we do not fully understand their functional diversities from an evolutionary perspective and we are not yet able to engineer new sequence specificities based on rational approaches. Recent findings on the evolution of RM systems and on their structures and mechanisms of action have led to a picture in which conserved modules with defined function are shared between different RM proteins and other enzymes involved in nucleic acid biochemistry. On the other hand, it has been realized that some of the modules have been replaced in the evolution by unrelated domains exerting similar function. The aim of this review is to give a survey on the recent progress in the field of structural phylogeny of RM enzymes with special emphasis on studies of sequence-structure-function relationships and emerging potential applications in biotechnology.
Cloning of the Haemophilus influenzae Dam methyltransferase and analysis of its relationship to the Dam methyltransferase encoded by the HP1 phage.
Authors: Bujnicki J M , Radlinska M , Zaleski P , Piekarowicz A
Published: December 2019

In this paper we report cloning and experimental characterization of the DNA adenine methyltransferase (dam) gene from Haemophilus influenzae and comparison of its product with the Dam protein from the lysogenic phage of H. influenzae, HP1. Molecular modeling of M.HinDam and M.HP1Dam was carried out, providing a framework for a comparative analysis of these enzymes and their close homologs in the structural context. Both proteins share the common fold and essential cofactor-binding and catalytic residues despite overall divergence. However, subtle but significant differences in the cofactor-binding pocket have been identified. Moreover, while M.HinDam seems to contact its target DNA sequence using a number of loops, most of them are missing from M.HP1Dam. Analysis of both MTases suggests that their catalytic activity was derived from a common ancestor, but similar sequence specificities arose by convergence.
Combined delivery of an antiangiogenic protein (angiostatin) and an immunomodulatory gene (interleukin-12) in the treatment of murine cancer.
Authors: Wilczyńska U , Kucharska A , Szary J , Szala S
Published: December 2019

We investigated the feasibility of a novel therapeutic approach to treat neoplastic diseases in mice. This novel strategy consists in delivering a protein (angiostatin) with strong antiangiogenic properties, followed by administration of the interleukin 12 gene that is strongly immunomodulatory and has also some antiangiogenic effects. When angiostatin-mediated antiangiogenic therapy was used in combination with intratumor delivery of the IL-12 gene (a strategy much safer than IL-12 protein administration), this produced a synergistic therapeutic effect.
Activity of glycogen depolymerizing enzymes in extracts from brain tumor tissue (anaplastic astrocytoma and glioblastoma multiforme).
Authors: Kotoński B , Wilczek J , Madej J , Zarzycki A , Hutny J
Published: December 2019

An approximately threefold increase in glycogenolytic activity of the neutral alpha-1,4-glucosidase and a twofold increase in the same activity of the acid isoform have been found in extracts of anaplastic astrocytoma and glioblastoma multiforme tumors of brain tissue. "Maltase activity" of the respective enzymes increased by 60-80% in both kinds of tumor extracts. However a significant decrease in a-amylase and almost complete disappearance of phosphorylase activities have also been found in both kinds of tumors.
Detection and sequencing of new cyclic peptides from linseed by electrospray ionization mass spectrometry.
Authors: Stefanowicz P
Published: December 2019

Extract of Linum usitatissimum seeds was analyzed by ESI-MS and ESI-MS/MS. The analysis confirms the presence of previously reported cyclolinopeptides: CLA (c(Pro-Pro-Phe-Phe-Leu-Ile-Ile-Leu-Val) and CLB (c(Pro-Pro-Phe-Phe-Val-Ile-Met-Ile-Leu)). Cyclolinopeptides CLD and CLE, which contain methionine oxide, were detected in small quantities only. These peptides likely result from the oxidation of their precursors, not reported previously: CLD' (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Leu)) and CLE'(c(Pro-Leu-Phe-Ile-Met-Leu-Val-Phe)), present at higher concentrations in unoxidized extract. Two new cyclic octapeptides: CLF (c(Pro-Phe-Phe-Trp-Val-Met-Leu-Met)) and CLG (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Met)) were detected and their sequences were proposed on the basis of CID experiments and similarity with those of CLD'.
Conformational stability of six truncated cHMG1a proteins studied in their mixture by H/D exchange and electrospray ionization mass spectrometry.
Authors: Petry I , Wigniewski J R , Szewczuk Z
Published: December 2019

The high mobility group (HMG) proteins are abundant non-histone components of eukaryotic chromatin. The presence of C-terminal acidic tails is a common feature of the majority of HMG proteins. Although the biological significance of the acidic domains is not clear, they are conferring conformational and metabolic stability to the proteins in vitro. Moreover, the length and net charge of the acidic tails affect the strength of HMG protein interaction with DNA. Synthesis of an insect HMG protein by standard recombinant technology in bacteria leads to a mixture of the intact protein (cHMG1a-(1-113) (I)) and a series of its degradation products truncated at the C tail: cHMG1a-(1-111) (II); cHMG1a-(1-110) (III); cHuMGla-(1-109) (IV); cHMG1a-(1-108) (V); cHMG1a-(1-107) (VI); cHMG1a-(1-106) (VII). The proteins differ from each other only by the number of amino-acid residues at the C-terminal tail. We used H/D exchange mass spectrometry to characterize the stability of the proteins directly in their mixture. The results show that the proteins I-V and VII have very similar conformations. The protein VI is less compact and exchanges its protons faster than the others. It may be concluded that the C-terminal tail influences the conformation of the cHMG1a protein and that individual residues in this part of the protein play a key role in its compactness.
Design, synthesis and biological evaluation of a new bridged immunosuppressor.
Authors: Szewczuk Z , Wilczyński A , Petry I , Siemion I Z , Wieczorek Z
Published: December 2019

A bridged peptide with the sequence: H-Thr-Pro-Gln-Arg-Gly-Asp-Val-gamma-Abu-Asn-Asp-Gln-Glu-Glu-Thr-Thr-Gly-Val-Val-Ser-Thr-Pro-Leu-Ile-Arg-Asn-Gly-OH was designed to mimic the discontinuous epitope of the HLA-DQ molecule that might interact with CD4. The bridged peptide revealed distinct suppressory effect in the humoral immune response. This result supports our suggestion that the 164-172 region of the HLA-DQ molecule may enhance its interactions with coreceptors, possibly with CD4.
Novel nociceptin analogues.
Authors: Bartosz-Bechowski H , Miedzybrodzki R , Szymaniec S
Published: December 2019

A series of new nociceptin analogues containing cysteine was tested for their nociceptive effects in tail-flick test on mice after icv injection. The cysteines were introduced in order to get irreversibly binding analogues based on the assumption that the cysteines in the ligand can interact with the cysteines from the receptor to form an S-S bridge. In vivo tests revealed that Cys1-nociceptin (1-13)-NH2 (Cys1-NC) is an antagonist, whereas Cys7-NC is an agonist. Gly1[Phe(p-NO2)]4-NC was less active indicating that the antagonist properties of Cys1-NC are associated with the presence of the sulfhydryl group of cysteine. The analogues D-Cys2 and Cys3 were also almost inactive.
Antibacterial peptides of the moth Galleria mellonella.
Authors: Mak P , Chmiel D , Gacek G J
Published: December 2019

The work describes purification and biochemical characterization of two inducible antimicrobial peptides from the hemolymph of Galleria mellonella. The peptides were isolated by a sequence of reversed-phase chromatography steps from the hemolymph of larvae immunized with viable bacteria. The first peptide is a member of the cecropin family while the second one is rich in proline residues and has a unique sequence.
Signal transmission via G protein-coupled receptors in the light of rhodopsin structure determination.
Authors: Ciarkowski J , Drabik P , Giełdoń A , Kaźmierkiewicz R , Slusarz R
Published: December 2019

G protein-coupled receptors (GPCRs) transducing diverse external signals to cells via activation of heterotrimeric GTP-binding (G) proteins, estimated to mediate actions of 60% of drugs, had been resistant to structure determination until summer 2000. The first atomic-resolution experimental structure of a GPCR, that of dark (inactive) rhodopsin, thus provides a trustworthy 3D prototype for antagonist-bound forms of this huge family of proteins. In this work, our former theoretical GPCR models are evaluated against the new experimental template. Subsequently, a working hypothesis regarding the signal transduction mechanism by GPCRs is presented.
The need for a practitioners' social media code of conduct in Iran
Authors: Sofi-Mahmudi Ahmad
Published: December 2019

This article is a Letter to the Editor and does not include an Abstract.
Professional socialization: an analytical definition
Authors: Sadeghi Avval Shahr Homa , Yazdani Shahram , Afshar Leila
Published: December 2019

Professional socialization is defined as a process through which a person becomes a legitimate member of a professional society. This will have a great impact on an individual’s professional conduct and morality. The aim of this study was to clarify this concept and reduce the ambiguities around it. This was a qualitative research through which the concept of professional socialization was analyzed using Walker and Avant’s eight-step approach. The review of literature for this concept was done using electronic database without any time limitation. The overall search produced about 780 articles, and after reviewing these articles, 21 were selected purposefully. Based on concept analysis, we propose the following analytical definition: Professional socialization is a nonlinear, continuous, interactive, transformative, personal, psychosocial and selfreinforcing process that is formed through internalization of the specific culture of a professional community, and can be affected by individual, organizational and interactional factors. This definition is in accordance with the interactionism perspective. Existence of a particular profession and getting involved in a community of practice are the antecedents of this process, and formation of professional identity and professional development are its consequences. A case model, as well as borderline and related cases, has been introduced for this concept. The results of this study can be used to design useful educational interventions to conduct and facilitate the process.
Relationship between Paraoxonase-1 and Arylesterase Enzyme Activities and SYNTAX I and II Scores in Patients with ST-Elevation Myocardial Infarction
Authors: Sökmen Erdoğan , Çelik Mustafa , Sivri Serkan , Güçlü Kenan
Published: December 2019

Background: Although serum paraoxonase-1 (PON-1) and arylesterase (ARE) activities are linked to the presence of stable coronary arterial disease, their correlation with SYNTAX Score I (SS1) and SYNTAX Score II (SS2) has not been known well. Our aim was to determine the association between PON-1 and ARE activities, together with the genetic polymorphism of PON-1, and both SS1 and SS2 in patients with acute ST-segment elevation myocardial infarction (STEMI).
Methods: Consecutive patients with acute STEMI (n=102: 78 male, 24 female; mean age=61.14±12.25 y) admitted to the Emergency Department of Kırşehir Ahi Evran University Hospital between August 2018 and December 2018 were enrolled. PON-1 and ARE activities were determined on hospital admission. The SS1 and SS2 scores were calculated by using the angiographic and clinical data. Subsequently, the relationships between the activities of the enzymes, together with the genetic polymorphism of PON-1, and both SS1 and SS2 were interrogated.
Results: The mean SS1 and the mean SS2 were 19.8±9.7 and 32.3±11.5, respectively. The phenotype distributions of PON-1 were Q192Q (n=60), R192Q (n=35), and R192R (n=7). The respective PON-1 (U/L) and ARE (kU/L) activities were 514.85±29.34 and 216.82±36.72 in the low SS1 category; 527.60±56.31 and 203.95±55.97 in the intermediate SS1 category; and, 690.10±11.07 and 238.48±45.65 in the high SS1 category.PON-1 and ARE activities did not correlate with the SS1 categories, and varying SS2 scores. The distribution of the Q192R polymorphism was homogenous among the different SS1 and SS2 scores. The localization of acute STEMI also did not associate with the activities of either enzyme.
Conclusion: Admission serum PON-1 and ARE activities, together with the PON-1 Q192R genetic polymorphism, showed association neither with SS1 and SS2 nor with the localization of infarction in our acute STEMI patients.
 
 
J Teh Univ Heart Ctr 2019;14(4):156-164
 
This paper should be cited as: Sökmen E, Çelik M, Sivri S, Güçlü K. Relationship between Paraoxonase-1 and Arylesterase Enzyme Activities and SYNTAX I and II Scores in Patients with ST-Elevation Myocardial Infarction. J Teh Univ Heart Ctr 2019;14(4):156-164.
Use of the Left Ventricular Internal Dimension at End-Diastole and the E-Point Septal Separation Ratio in the Prediction of the Left Ventricular Systolic Function in Patients with Midrange and Reduced Ejection Fractions: A Pilot Study
Authors: Cosansu Kahraman , Kilic Harun , Cabbar Ayca Turer , Hatipsoylu Erdinc , Karadag Bilgehan , Akdemir Ramazan
Published: December 2019

Background: The aim of this study was to investigate the ability of a new index, namely the left ventricular internal dimension at end-diastole/mitral valve E-point septal separation (LVIDd/EPSS), to predict the left ventricular (LV) systolic function and to compare its performance with that of the EPSS index and to investigate the correlation between the LVIDd/EPSS and the left ventricular ejection fraction (LVEF).
Methods: The current study recruited 142 patients who presented to the Cardiology Clinic of Sakarya University Education and Research Hospital and were followed for heart failure (HF).M-mode measurements of the EPSS and the LVIDd were recorded in the parasternal long-axis view.
Results: Totally, 142 HF patients with midrange ejection fraction (HFmrEF) and reduced ejection fraction (HFrEF) were enrolled in the study. There was a significantly correlation both between the EF and the EPSS and between the EF and the LVIDd/EPSS (P<0.001). In both HFmrEF and HFrEF groups, the correlation between the LVIDd/EPSS and the EF was more significant than was the correlation between the EPSS and the EF (P<0.001). The results of the linear regression analysis indicated that the LVIDd/EPSS was an independent predictor of the HFmrEF and the HFrEF (P<0.001). In the patients with EPSS≤12, there was a significant association between the EF and the LVIDd/EPSS (P<0.001) but not between the EF and the EPSS(P>0.05). The receiver operating characteristic curve analysis showed that the LVIDd/EPSS predicted advanced HF with 87% sensitivity and 72% specificity, using a cutoff value of 3.35,and it predicted the HFrEF (EF<40%) with 84% sensitivity and 81% specificity, using a cutoff value of 3.75.
Conclusion: The LVIDd/EPSS may allow certain clinicians, especially beginners and emergency department physicians, to assess the LVEF when other methods are not available or questionable­.
 
J Teh Univ Heart Ctr 2019;14(4):171-176
 
This paper should be cited as: Cosansu K, Kilic H, Turer Cabbar A, Hatipsoylu E, Karadag B, Akdemir R. Use of the Left Ventricular Internal Dimension at End-Diastole and the E-Point Septal Separation Ratio in the Prediction of the Left Ventricular Systolic Function in Patients with Midrange and Reduced Ejection Fractions: A Pilot Study. J Teh Univ Heart Ctr 2019;14(4):171-176.
Effect of the Aortic Root Infusion of Sufentanil on Ischemia-Reperfusion Injury in Patients Undergoing Coronary Artery Bypass Grafting: A Randomized Clinical Trial
Authors: Khosravi Mohammad Bagher , Kahrom Mahdi , Tahari Mahdi , Alizadeh Kambiz , Soltani Ghasem , Ghanad Mohammad Ali
Published: December 2019

Background: Ischemic postconditioning is a novel strategy for attaining cardioprotection. Remarkable evidence from various in vitro and in vivo animal and human studies have shown significant opioid-induced cardioprotection against myocardial ischemia/reperfusion (I/R) injury. The purpose of this study was to assess the cardioprotective effect of sufentanil against I/R injury after on-pump coronary artery bypass grafting (CABG).
Methods: Between June 2016 and July 2017, 80 consecutive patients with triple-vessel disease undergoing on-pump CABG were enrolled in this prospective randomized study. The patients assigned to the sufentanil group received a single dose of sufentanil (0.2 μg/kg diluted with 50 cc of saline) 5 minutes before the removal of the aorta cross-clamp, with the sufentanil injected via a cardioplegia needle into the aortic root. In the control group, the same volume of normal saline was injected as a placebo. Cardiac enzymes, the inotrope score, and the outcome data were compared between the 2 groups.
Results: The mean age of the patients was 60.48±7.50 years (range=41–69 y), and men comprised 65.0% of the study population. The levels of CK-MB and cardiac troponin I were significantly lower in the sufentanil group (P<0.001). The amount of inotrope use (P<0.001), the incidence of atrial fibrillation (P=0.014), electrical shock (P=0.007), and the mechanical ventilation time (P<0.001) decreased in the sufentanil group compared with the control group. However, the use of intra-aortic balloon pumps (P=0.247) and the ICU length of stay (P=0.867) were not significantly different between the 2 groups.
Conclusion: The injection of a single dose of sufentanil into the aortic root prior to aorta cross-clamp removal diminished cardiac injury during on-pump CABG in our patients.
 
J Teh Univ Heart Ctr 2019;14(4):177-182
 
This paper should be cited as: Bagher Khosravi M, Kahrom M, Tahari M, Alizadeh K, Soltani G, Ghanad MA. Effect of the Aortic Root Infusion of Sufentanil on Ischemia-Reperfusion Injury in Patients Undergoing Coronary Artery Bypass Grafting: A Randomized Clinical Trial. J Teh Univ Heart Ctr 2019;14(4):177-182.
Acute Dissection of the Ascending Aorta as a Rare Complication of Aortocoronary Bypasses Surgery: A Case Report
Authors: Borulu Ferhat , Erkut Bilgehan
Published: December 2019

Many complications may ensue coronary bypass surgery. Among these complications, early or late dissection of the ascending aorta is a very rare condition. Successful surgery without life-threatening results can save lives. In this case report, we present a case of aortic dissection due to cross-clamp injury in a patient who underwent coronary artery bypass surgery.
A 60-year-old female patient underwent double-vessel aortocoronary bypass surgery due to coronary artery disease. After distal bypasses were performed and the cross-clamp was removed, dissection of the aorta occurred. Consequently, axillary cannulation and ascending aortic replacement with a prosthetic graft were performed via the open technique. The saphenous vein graft was sutured to the aortic prosthetic graft, and cardiopulmonary bypass was terminated after hemodynamic stability was achieved. The patient was discharged on the 10th postoperative day, and she has been followed up for 2 years after surgery without any problems.
 
J Teh Univ Heart Ctr 2019;14(4):191-194
 
This paper should be cited as: Borulu F, Erkut B. Acute Dissection of the Ascending Aorta as a Rare Complication of Aortocoronary Bypasses Surgery: A Case Report. J Teh Univ Heart Ctr 2019;14(4):191-194.
Prediabetes and Right Ventricular Function: What Should Be Done for Clarification?
Authors: Davarpasand Tahereh
Published: December 2019

The article's abstract is no available.
Retraction Notice of an Article
Authors: Karimi Abbasali
Published: December 2019

The Journal of Tehran University Heart Center hereby declares the retraction of the article entitled “A Rare Case of Cardiogenic Shock following Severe Multivessel Coronary Vasospasm”, which was already published in this journal (J Teh Univ Heart Ctr 2019;14:28-32, DOI: https://doi.org/10.18502/jthc.v14i1.653).
This article has been retracted at the request of its authors, who stated that notwithstanding the merits of their reported case, the complicated manifestations of the patient require further causal investigation via a more scrutinized approach.