bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Figure 9 from The Immune Suppressor IGSF1 as a Potential Target for Cancer Immunotherapy
Authors: Koh Dong-In , Lee Minki , Park Yoon Sun , Shin Jae-Sik , Kim Joseph , Ryu Yea Seong , Lee Jun Hyung , Bae Seunggeon , Lee Mi So , Hong Jun Ki , Jeong Hong-Rae , Choi Mingee , Hong Seung-Woo , Kim Dong Kwan , Lee Hyun-kyung , Kim Bomi , Yoon Yoo Sang , Jin Dong-Hoon
Published: April 2024

The combination efficacy of anti-PD-1 and WM-A1 in cancer-PBCM co-culture assay.
Supplementary Figure 4 from The Immune Suppressor IGSF1 as a Potential Target for Cancer Immunotherapy
Authors: Koh Dong-In , Lee Minki , Park Yoon Sun , Shin Jae-Sik , Kim Joseph , Ryu Yea Seong , Lee Jun Hyung , Bae Seunggeon , Lee Mi So , Hong Jun Ki , Jeong Hong-Rae , Choi Mingee , Hong Seung-Woo , Kim Dong Kwan , Lee Hyun-kyung , Kim Bomi , Yoon Yoo Sang , Jin Dong-Hoon
Published: April 2024

Regulating of expression of Igsf1 is not critical for the proliferation, migration and invasion of cancer cells.
Supplementary Figure 4 from Tumor Burden Dictates the Neoantigen Features Required to Generate an Effective Cancer Vaccine
Authors: Garzia Irene , Nocchi Linda , Avalle Lidia , Troise Fulvia , Leoni Guido , Seclì Laura , Antonucci Laura , Cotugno Gabriella , Allocca Simona , Romano Giuseppina , Conti Laura , Caiazza Carmen , Mallardo Massimo , Poli Valeria , Scarselli Elisa , D'Alise Anna Morena
Published: April 2024

Characterization of neoAg#23 specific T cell subsets in the presence/ absence of T cell “Help”. A) Schematic representation of the experiment. Mice were s.c. injected with CT26 cells and 5 days later, immunized with GAd vaccines CD4+CD8#23 (Help) and CD8#23 (No Help) in combination with anti-PD1. At day 9 and day 14 post vaccination, tumors (B) and spleens (C) were collected for FACS analysis on antigen-specific nAg#23+ CD44+CD62- (T Effector memory, TEM). The percentage (%) of TEM and #23-specific T cells are shown. Two-tailed Mann-Whitney test versus CT26 tumor bearing mice, p-values * p<0.05; ns = not significant (Untreated n=4; Help day 9 n= 6; no Help day 9 n=6; Help day 14 n=7; no Help n=7). D) Schematic representation of the experiment. Mice were s.c. injected with CT26 cells and 5 days later, immunized with GAd vaccine CD4+CD8#23 (Help, n=5). E) Blood was collected at different time points post immunization from responder mice (RES) and (F) not responders (NR, n=5-14, depending on the time point) for FACS analysis of nAg#23+ specific CD8+ T-cells.
Supplementary Figure 3 from Tumor Burden Dictates the Neoantigen Features Required to Generate an Effective Cancer Vaccine
Authors: Garzia Irene , Nocchi Linda , Avalle Lidia , Troise Fulvia , Leoni Guido , Seclì Laura , Antonucci Laura , Cotugno Gabriella , Allocca Simona , Romano Giuseppina , Conti Laura , Caiazza Carmen , Mallardo Massimo , Poli Valeria , Scarselli Elisa , D'Alise Anna Morena
Published: April 2024

Primary tumor challenge with NeoAg #5 knocked out CT26 tumor cells. Mice were immunized with CD8#5 mono-epitope vaccine and after 14 days, mice were s.c. inoculated with CT26 cells or CT26 #5KO cells, resulting in 50% and 0% of tumor free mice, respectively. Bars show the percentage (%) of tumor free (grey) and tumor bearing (black) mice (n=8). No vaccinated mice (Untreated) were s.c. inoculated with CT26 #5KO cells as control (n=.6). One-tailed Fisher’s exact test CT26 vs CT26#5KO, p-values * p=0.03.
Supplementary Figure 1 from Tumor Burden Dictates the Neoantigen Features Required to Generate an Effective Cancer Vaccine
Authors: Garzia Irene , Nocchi Linda , Avalle Lidia , Troise Fulvia , Leoni Guido , Seclì Laura , Antonucci Laura , Cotugno Gabriella , Allocca Simona , Romano Giuseppina , Conti Laura , Caiazza Carmen , Mallardo Massimo , Poli Valeria , Scarselli Elisa , D'Alise Anna Morena
Published: April 2024

Mutation loss in developed CT26 tumors after GAd CD8#5 and CD8#23 vaccine. A) Mice were immunized with mono-epitope GAd CD8#5 or B) CD8#23 vaccines and 14 days later, mice were inoculated s.c. with 2x10^5 CT26 cells. Mutation relative to nAg#5 (A) and nAg#23 (B) was checked by DNA sequencing in developed tumors. C) Table shows the percentage of mutation loss relative to nAg#5 (33%, E2f8 gene) and nAg#23 (0%, Mtch1 gene) in CT26 developing tumors after immunization with CD8#5 or CD8#23 vaccine.
Supplementary Figure 4 from Immune Modulation with RANKL Blockade through Denosumab Treatment in Patients with Cancer
Authors: Chang Hewitt , Marquez Jaqueline , Chen Brandon K. , Kim Daniel M. , Cheng Michael L. , Liu Eric V. , Yang Hai , Zhang Li , Sinha Meenal , Cheung Alexander , Kwek Serena S. , Chow Eric D. , Bridge Mark , Aggarwal Rahul R. , Friedlander Terence W. , Small Eric J. , Anderson Mark , Fong Lawrence
Published: April 2024

Supplementary Figure 4: Pre-existing immune condition prior to denosumab treatment Heatmaps summarizing log2 fold changes from statistical analysis of functional markers CD137, CD27, CD40, CD40L, CD71, CD95, CTLA-4, HLA-DR, ICOS, Ki-67, NKG2D, OX40, PD-1, PD-L1, PD-L2, TIGIT and TIM3 when comparing patients who did not receive chemotherapy or steroids with patients that received chemotherapy, steroids, or both at pretreatment TP1. Each box in the heatmap represents a lymphocyte cluster that was labeled according to the nearest landmark node they connect to on the scaffold map and ordered by cell count abundance. The pseudocolor represents clusters that showed a significant difference (q<0.05) in the log2 fold change, with red being significantly higher, while blue being significantly lower in group 2 of the two comparison groups.
Supplementary Figure Legends and Tables 2-9 from Gut Mycobiota Dysbiosis Is Associated with Melanoma and Response to Anti–PD-1 Therapy
Authors: Szóstak Natalia , Handschuh Luiza , Samelak-Czajka Anna , Tomela Katarzyna , Pietrzak Bernadeta , Schmidt Marcin , Galus Łukasz , Mackiewicz Jacek , Mackiewicz Andrzej , Kozlowski Piotr , Philips Anna
Published: April 2024

Supplementary Figure Legends and Tables 2-9
Supplementary Figure 11 from Gut Mycobiota Dysbiosis Is Associated with Melanoma and Response to Anti–PD-1 Therapy
Authors: Szóstak Natalia , Handschuh Luiza , Samelak-Czajka Anna , Tomela Katarzyna , Pietrzak Bernadeta , Schmidt Marcin , Galus Łukasz , Mackiewicz Jacek , Mackiewicz Andrzej , Kozlowski Piotr , Philips Anna
Published: April 2024

Supplementary Figure 11. Relative abundance of selected species in responders and non responders (R vs. NR).
Supplementary Figure 10 from Gut Mycobiota Dysbiosis Is Associated with Melanoma and Response to Anti–PD-1 Therapy
Authors: Szóstak Natalia , Handschuh Luiza , Samelak-Czajka Anna , Tomela Katarzyna , Pietrzak Bernadeta , Schmidt Marcin , Galus Łukasz , Mackiewicz Jacek , Mackiewicz Andrzej , Kozlowski Piotr , Philips Anna
Published: April 2024

Supplementary Figure 10. Relative abundance of selected species in patients that exhibited benefits during anti-PD-1 treatment and patients who did not show benefits (CB vs. NB).
Supplementary Figure 1 from Gut Mycobiota Dysbiosis Is Associated with Melanoma and Response to Anti–PD-1 Therapy
Authors: Szóstak Natalia , Handschuh Luiza , Samelak-Czajka Anna , Tomela Katarzyna , Pietrzak Bernadeta , Schmidt Marcin , Galus Łukasz , Mackiewicz Jacek , Mackiewicz Andrzej , Kozlowski Piotr , Philips Anna
Published: April 2024

Supplementary Figure 1. Baseline characteristics of the study populations with respect to (A) age groups, (B) BMI groups, and (C) sex.
Supplementary Figure 5 from Dual Chimeric Antigen Receptor T Cells Targeting CD38 and SLAMF7 with Independent Signaling Demonstrate Preclinical Efficacy and Safety in Multiple Myeloma
Authors: Roders Nathalie , Nakid-Cordero Cecilia , Raineri Fabio , Fayon Maxime , Abecassis Audrey , Choisy Caroline , Nelson Elisabeth , Maillard Claire , Garrick David , Talbot Alexis , Fermand Jean-Paul , Arnulf Bertrand , Bories Jean-Christophe
Published: April 2024

Supplementary Figure 5
Supplementary Figure 4 from Dual Chimeric Antigen Receptor T Cells Targeting CD38 and SLAMF7 with Independent Signaling Demonstrate Preclinical Efficacy and Safety in Multiple Myeloma
Authors: Roders Nathalie , Nakid-Cordero Cecilia , Raineri Fabio , Fayon Maxime , Abecassis Audrey , Choisy Caroline , Nelson Elisabeth , Maillard Claire , Garrick David , Talbot Alexis , Fermand Jean-Paul , Arnulf Bertrand , Bories Jean-Christophe
Published: April 2024

Supplementary Figure 4
Supplementary Figure 3 from Dual Chimeric Antigen Receptor T Cells Targeting CD38 and SLAMF7 with Independent Signaling Demonstrate Preclinical Efficacy and Safety in Multiple Myeloma
Authors: Roders Nathalie , Nakid-Cordero Cecilia , Raineri Fabio , Fayon Maxime , Abecassis Audrey , Choisy Caroline , Nelson Elisabeth , Maillard Claire , Garrick David , Talbot Alexis , Fermand Jean-Paul , Arnulf Bertrand , Bories Jean-Christophe
Published: April 2024

Supplementary Figure 3
Supplementary Figure 2 from Dual Chimeric Antigen Receptor T Cells Targeting CD38 and SLAMF7 with Independent Signaling Demonstrate Preclinical Efficacy and Safety in Multiple Myeloma
Authors: Roders Nathalie , Nakid-Cordero Cecilia , Raineri Fabio , Fayon Maxime , Abecassis Audrey , Choisy Caroline , Nelson Elisabeth , Maillard Claire , Garrick David , Talbot Alexis , Fermand Jean-Paul , Arnulf Bertrand , Bories Jean-Christophe
Published: April 2024

Supplementary Figure 2
Supplementary Table S1 from NCI Resources for Cancer Immunoprevention Research
Authors: Sei Shizuko , Srivastava Sudhir , Kelly Halonna R. , Miller Mark Steven , Leitner Wolfgang W. , Shoemaker Robert H. , Szabo Eva , Castle Philip E.
Published: April 2024

Supplementary Table S1: List of Program Officers for the programs discussed in this article.
Supplementary Tables from High-Specificity CRISPR-Mediated Genome Engineering in Anti-BCMA Allogeneic CAR T Cells Suppresses Allograft Rejection in Preclinical Models
Authors: Degagné Émilie , Donohoue Paul D. , Roy Suparna , Scherer Jessica , Fowler Tristan W. , Davis Ryan T. , Reyes Gustavo A. , Kwong George , Stanaway Morena , Larroca Vicena Vanina , Mutha Devin , Guo Raymond , Edwards Leslie , Schilling Benjamin , Shaw McKay , Smith Stephen C. , Kohrs Bryan , Kufeldt Heinrich J. , Churchward Glen , Ruan Finey , Nyer David B. , McSweeney Kyle , Irby Matthew J. , Fuller Christopher K. , Banh Lynda , Toh Mckenzi S. , Thompson Matthew , Owen Arthur L.G. , An Zili , Gradia Scott , Skoble Justin , Bryan Mara , Garner Elizabeth , Kanner Steven B.
Published: April 2024

Supplementary Tables S1 through S11
Figure S7. from High-Specificity CRISPR-Mediated Genome Engineering in Anti-BCMA Allogeneic CAR T Cells Suppresses Allograft Rejection in Preclinical Models
Authors: Degagné Émilie , Donohoue Paul D. , Roy Suparna , Scherer Jessica , Fowler Tristan W. , Davis Ryan T. , Reyes Gustavo A. , Kwong George , Stanaway Morena , Larroca Vicena Vanina , Mutha Devin , Guo Raymond , Edwards Leslie , Schilling Benjamin , Shaw McKay , Smith Stephen C. , Kohrs Bryan , Kufeldt Heinrich J. , Churchward Glen , Ruan Finey , Nyer David B. , McSweeney Kyle , Irby Matthew J. , Fuller Christopher K. , Banh Lynda , Toh Mckenzi S. , Thompson Matthew , Owen Arthur L.G. , An Zili , Gradia Scott , Skoble Justin , Bryan Mara , Garner Elizabeth , Kanner Steven B.
Published: April 2024

CB-011 immune-cloaking armoring strategy suppresses cytotoxic T and NK cell–mediated allograft rejection.
Figure S5. from High-Specificity CRISPR-Mediated Genome Engineering in Anti-BCMA Allogeneic CAR T Cells Suppresses Allograft Rejection in Preclinical Models
Authors: Degagné Émilie , Donohoue Paul D. , Roy Suparna , Scherer Jessica , Fowler Tristan W. , Davis Ryan T. , Reyes Gustavo A. , Kwong George , Stanaway Morena , Larroca Vicena Vanina , Mutha Devin , Guo Raymond , Edwards Leslie , Schilling Benjamin , Shaw McKay , Smith Stephen C. , Kohrs Bryan , Kufeldt Heinrich J. , Churchward Glen , Ruan Finey , Nyer David B. , McSweeney Kyle , Irby Matthew J. , Fuller Christopher K. , Banh Lynda , Toh Mckenzi S. , Thompson Matthew , Owen Arthur L.G. , An Zili , Gradia Scott , Skoble Justin , Bryan Mara , Garner Elizabeth , Kanner Steven B.
Published: April 2024

Antitumor activity of CB-011 CAR-T cells in a xenograft model of multiple myeloma.
Supplementary Figure S2 from Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells
Authors: Carnevale Silvia , Ponzetta Andrea , Rigatelli Anna , Carriero Roberta , Puccio Simone , Supino Domenico , Grieco Giovanna , Molisso Piera , Di Ceglie Irene , Scavello Francesco , Perucchini Chiara , Pasqualini Fabio , Recordati Camilla , Tripodo Claudio , Belmonte Beatrice , Mariancini Andrea , Kunderfranco Paolo , Sciumè Giuseppe , Lugli Enrico , Bonavita Eduardo , Magrini Elena , Garlanda Cecilia , Mantovani Alberto , Jaillon Sebastien
Published: April 2024

Figure S2. Neutrophil deficiency is associated with intestinal dysbiosis. Related to Figure 2
A) Representative histological images of H&E-stained colon section from Csf3r+/+(left) and Csf3r-/- (right) mice, after one cycle of DSS, showing the increased inflammatory infiltrate and the presence of bacterial accretions (indicated by black arrow) in Csf3r-/-. B-C) Shannon Index representing the evenness in the overall number of bacterial species between wild-type and neutrophil deficient mice untreated (B) and DSS-treated mice (C). D-E) Taxonomic analysis of the phyla that compose the microbiota of feces of untreated (D) and DSS-treated (E) Csf3r+/+ (untreated n=4, DSS-treated n=3) and Csf3r-/- (untreated n=4, DSS-treated n=4) mice. F) Microbiota depletion efficiency measured by qPCR for 16S gene: complete depletion is achieved after 20 days of antibiotic treatment. G) Body weight loss during DSS-induced acute colitis in Csf3r+/+(n=11) and Csf3r-/- (n=9) mice with and without cohousing (Csf3r+/+Cohoused n=5 and Csf3r-/-Cohoused n=5). H) Body weight loss of AOM/DSS treated Csf3r+/+(n=7) and Csf3r-/- (n=5) mice with and without cohousing (Csf3r+/+Cohoused n=5 and Csf3r-/-Cohoused n=5); I) Macroscopic polyp count at the experimental endpoint. A-I) One experiment. B-C) Unpaired Student’s t-Test. F, I) Multiple Student’s t-Test. G-H) Wilcoxon matched-pairs signed rank test. Data are mean ± SEM. *** p < 0.001 ** p < 0.01 * p < 0.05.
Supplementary Figure S1 from Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells
Authors: Carnevale Silvia , Ponzetta Andrea , Rigatelli Anna , Carriero Roberta , Puccio Simone , Supino Domenico , Grieco Giovanna , Molisso Piera , Di Ceglie Irene , Scavello Francesco , Perucchini Chiara , Pasqualini Fabio , Recordati Camilla , Tripodo Claudio , Belmonte Beatrice , Mariancini Andrea , Kunderfranco Paolo , Sciumè Giuseppe , Lugli Enrico , Bonavita Eduardo , Magrini Elena , Garlanda Cecilia , Mantovani Alberto , Jaillon Sebastien
Published: April 2024

Figure S1. Neutrophil deficiency is associated with increased lymphoid aggregates. Related to Figure 1.A) Number of total lymphoid structures and B) number of lymphoid structures with follicular dendritic cells detected by RNAscope staining for the follicular dendritic cell marker Mfge8 in colon tissue sections from Csf3r+/+(n=8) and Csf3r-/- (n=5) mice after AOM/DSS-induced CRC. C) Representative images of RNAscope analysis for Mfge8 in colon tissue sections from Csf3r-/- mice after AOM/DSS-induced CRC: aggregates with follicular structures are visible as Mfge8+ (red spot): on the top is visible a lymphoid aggregate at two different magnifications, with diffuse Mfge8+ cells; on the bottom right lymphoid microaggregate with Mfge8+ cells; on the bottom left aggregate without follicular structures (Mfge8+). D) Representative dot-plot of neutrophils frequency in blood (top panels) and colon LP (bottom panels) of Csf3r-/- mice and Csf3r-/- mice 4hrs and 18hrs after adoptive transfer of neutrophils. E-F) Frequency of neutrophils in blood (D) and colon LP (E) of DSS-treated Csf3r-/- mice (n=5) and Csf3r-/- mice 4hrs (n=5) and 18hrs (n=5) after adoptive transfer of neutrophils. A-B) Representative data of three independent experiments. C-E) One experiment. A-B, D-E) Unpaired Student’s t-Test. Data are mean ± SEM. *** p < 0.001 ** p < 0.01 * p < 0.05.
Supplementary Table S3 from Reprogramming the Intrahepatic Cholangiocarcinoma Immune Microenvironment by Chemotherapy and CTLA-4 Blockade Enhances Anti–PD-1 Therapy
Authors: Chen Jiang , Amoozgar Zohreh , Liu Xin , Aoki Shuichi , Liu Zelong , Shin Sarah M. , Matsui Aya , Hernandez Alexei , Pu Zhangya , Halvorsen Stefan , Lei Pin-Ji , Datta Meenal , Zhu Lingling , Ruan Zhiping , Shi Lei , Staiculescu Daniel , Inoue Koetsu , Munn Lance L. , Fukumura Dai , Huang Peigen , Sassi Slim , Bardeesy Nabeel , Ho Won Jin , Jain Rakesh K. , Duda Dan G.
Published: April 2024

Supplementary Table S3: IMC panel.
Supplementary Fig. S6 from Reprogramming the Intrahepatic Cholangiocarcinoma Immune Microenvironment by Chemotherapy and CTLA-4 Blockade Enhances Anti–PD-1 Therapy
Authors: Chen Jiang , Amoozgar Zohreh , Liu Xin , Aoki Shuichi , Liu Zelong , Shin Sarah M. , Matsui Aya , Hernandez Alexei , Pu Zhangya , Halvorsen Stefan , Lei Pin-Ji , Datta Meenal , Zhu Lingling , Ruan Zhiping , Shi Lei , Staiculescu Daniel , Inoue Koetsu , Munn Lance L. , Fukumura Dai , Huang Peigen , Sassi Slim , Bardeesy Nabeel , Ho Won Jin , Jain Rakesh K. , Duda Dan G.
Published: April 2024

Supplementary Fig. S6: CTLA-4 blockade mediates the efficacy of GC/ICB therapy in ICC and increases CD8+CTL frequency in murine ICC.
Data from Gut Mycobiota Dysbiosis Is Associated with Melanoma and Response to Anti–PD-1 Therapy
Authors: Szóstak Natalia , Handschuh Luiza , Samelak-Czajka Anna , Tomela Katarzyna , Pietrzak Bernadeta , Schmidt Marcin , Galus Łukasz , Mackiewicz Jacek , Mackiewicz Andrzej , Kozlowski Piotr , Philips Anna
Published: April 2024

Abstract
Recent research indicates that gut microbiota may be vital in the advancement of melanoma. In this study, we found that melanoma patients exhibited a distinct gut mycobiota structure compared with healthy participants. Candida albicans, Candida dubliniensis, and Neurospora crassa were more abundant in samples from patients with melanoma, whereas Saccharomyces cerevisiae and Debaryomyces hansenii were less abundant. During anti–PD-1 treatment, the relative amount of Malassezia restricta and C. albicans increased. A higher level of Saccharomyces paradoxus was associated with a positive response to anti–PD-1 treatment, whereas a higher level of Tetrapisispora blattae was associated with a lack of clinical benefits. High levels of M. restricta and C. albicans, elevated serum lactate dehydrogenase, and being overweight were linked to increased risk of melanoma progression and poorer response to anti–PD-1 treatment. Thus, this study has revealed melanoma-associated mycobiome dysbiosis, characterized by altered fungal composition and fungi species associated with a higher risk of melanoma progression, identifying a role for the gut mycobiome in melanoma progression.
Figure S3 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen
Authors: Zhou Shiqi , Song Yiting , Luo Yuan , Quinn Breandan , Jiao Yang , Long Mark D. , Abrams Scott I. , Lovell Jonathan F.
Published: April 2024

p15E-3C2V immunized mice showed long-term resistance to MC38 tumor re-challenge
FIGURE 5 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen
Authors: Zhou Shiqi , Song Yiting , Luo Yuan , Quinn Breandan , Jiao Yang , Long Mark D. , Abrams Scott I. , Lovell Jonathan F.
Published: April 2024

p15E-3C mimotope-induced inhibition of MC38 tumor growth is CD8+ T-cell dependent. Mice received 2 µg p15E-3C liposomal vaccine on days 0 and 7 before challenge with 1 × 106 MC-38 cells on day 14 (n = 6). A, Schedule of injections and tumor inoculation. Mice received 400 µg CD8 MAb or CD4 MAb on day 12 and day 13 as indicated; 200 µg CD8 mAb or CD4 mAb on day 18 and day 22 as indicated. B, Average tumor volume of indicated groups. C, Percentage of mice with tumor length shorter than 10 mm. D, Tumor volumes of individual mice from indicated groups. Datapoints represent mean values of 6 mice from the same group for B. Error bars show mean − SD.
FIGURE 3 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen
Authors: Zhou Shiqi , Song Yiting , Luo Yuan , Quinn Breandan , Jiao Yang , Long Mark D. , Abrams Scott I. , Lovell Jonathan F.
Published: April 2024

The p15E-3M mimotope induces improved cytotoxic T-cell responses in vitro. A, Mean fluorescence intensity (MFI) of the indicated peptides bound to RMA-S cells after staining with PE-labeled anti-mouse H-2Kb antibody. B, Binding stability of the indicated peptide with RMA-S MHC-I molecules. Mice were immunized with 2 µg of the indicated peptide liposomal vaccine per injection on days 0 and day 7. C, The percentage of p15E-specific CD8+ T cells in all CD8+ T cells from blood tested on day 14. Splenocytes were collected on day 14 for cytotoxic CD8+ T-cell generation and lytic activity evaluation from the indicated groups (D). Splenocytes were stimulated with the different peptides as indicated. E, IFNγ ELISPOT assays were recorded. Datapoints represent mean values of 3 mice from the same group for A, B, and E; represent mean values of four mice for D. Datapoints represent individual values for each mouse for C. Statistical analysis applied one-way ANOVA followed by Tukey multiple comparisons tests. *, **, ***, and **** indicating P < 0.05, 0.01, 0.001, and 0.0001, or respectively compared with the 3M group in E; #,### indicating P < 0.05, 0.001, respectively compared with 3C group in E. Error bars show mean +, ±, or − SD.
FIGURE 2 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen
Authors: Zhou Shiqi , Song Yiting , Luo Yuan , Quinn Breandan , Jiao Yang , Long Mark D. , Abrams Scott I. , Lovell Jonathan F.
Published: April 2024

Functional screening of positional peptide microlibraries reveals 3M and 3C substitutions as p15E e-mimotopes. A, Schematic shows the strategy of two-step e-mimotope screening. Mice were vaccinated with peptide position library vaccines (B) or individual peptide vaccines (C) with the position 3 amino acid being replaced, as indicated. Vaccinations occurred on days 0 and day 7, followed by inoculation of 1 × 106 MC38 cells on day 14. Tumor volume on day 19 after tumor inoculation was recorded. The square symbol in C indicates the mouse was euthanized because of tumor ulceration before day 19. Size (D) and PDI (E) of CPQ liposomes with the indicated peptide. F, Binding of peptides to PoP (lacking cobalt) or CoPoP liposomes after incubation at room temperature for 1 hour. Statistical analysis applied using the unpaired t test. ****, P < 0.0001. Error bars show mean + SD for three independent experiments.
The Cystic Sponge Anus
Authors: van der Zee Hessel H. , Aarts Pim
Published: April 2024

Introduction: A peculiar spongy appearance of the perianal skin was observed in a patient who underwent wide excision surgery for inguinal and gluteal hidradenitis suppurativa (HS). Case Presentation: This peculiar appearance was observed in a 62-year-old male patient. It included multiple orifices and cavities forming the spongy aspect of the perianal skin with multiple cysts and giant comedones. The perianal lesions were asymptomatic and the patient had never received any perianal treatment for the comedones or cysts. Histopathology was performed and demonstrated dilated hair follicles with flaky keratin and loose hair shafts in the center. The spongy appearance was most likely the result of spontaneous shedding of the cystic contents. Conclusion: We suspect that the “cystic sponge anus” might be associated with HS, smoking, the male gender, and may yet be another expression of an occlusive follicular disease. Future studies will be needed to clarify the prevalence and comorbidities of the “cystic sponge anus.”
Sclerectomy Reverses Nanophthalmic Optic Neuropathy
Authors: Mansour Ahmad M. , Uwaydat Sami H. , Hamam Rola , Salti Haytham I.
Published: April 2024

Introduction: Nanophthalmos is characterized by a short axial length, a thick choroid, and a thick sclera. Unilateral symptomatic disc swelling in nanophthalmos presents both a diagnostic and a therapeutic challenge. Case Presentation: A healthy 59-year-old man reported a two-week-long abrupt vision reduction in his right eye. 20/100 best spectacle (+17.25 diopter) corrected visual acuity, unilateral widespread disc enlargement, central scotoma, and a slight color vision disruption without an afferent pupillary defect were among the positive findings in the right eye. Workup for neuro-ophthalmology was negative. Numerous consultations did not suggest any form of treatment for the patient. Review of the optical coherence tomography (OCT) indicated a small, crowded optic nerve head and substantial diffuse choroidal thickening with dome-shaped temporal peripapillary area with choroidal expansion. In addition to circumferential anterior four-quadrant 95%-deep sclerectomy from recti insertion to the vortices, radial nasal posterior sclerotomy reaching the optic nerve sheath was performed on the patient. After the procedure, 2 weeks later, the patient’s vision returned, and it persisted until the 6-month follow-up. By OCT, the two eyes were comparable as far as disc contour and nerve fiber layer thickness. Conclusion: This form of sclerectomy, which aims at decompressing the oncotic choroidal pressure, is an effective treatment for compressive optic neuropathy in the context of nanophthalmos. Could sclerectomy assist in treating other optic neuropathies associated with peripapillary pachychoroid?
Displacement of Submacular Hemorrhage Using Subretinal Cocktail Injection versus Pneumatic Displacement: A Real-World Comparative Study
Authors: Szeto Simon K.H. , Tsang Chi Wai , Mohamed Shaheeda , Lee Gary K.Y. , Lok Jerry K.H. , Hui Vivian W.K. , Tsang Ken K. , Chen Li Jia , Brelen Marten , Lai Timothy Y.Y.
Published: April 2024

Introduction: The objective of this study was to compare the outcome of submacular hemorrhage (SMH) displacement using pneumatic displacement with intravitreal expansile gas versus pars plana vitrectomy (PPV) with subretinal injection of tissue plasminogen activator (tPA), anti-vascular endothelial growth factor (VEGF) agent, and air as primary surgery. Methods: Retrospective interventional case series of 63 patients who underwent surgical displacement of SMH secondary to neovascular age-related macular degeneration (nAMD) or polypoidal choroidal vasculopathy (PCV) from May 1, 2015, to October 31, 2022. Medical records were reviewed for diagnosis, logMAR visual acuity (VA), central subfield thickness (CST), and postoperative displacement rates and complications up to 12 months after operation. Results: The diagnosis was nAMD in 24 (38.1%) and PCV in 39 (61.9%) eyes. There were 40 (63.5%) eyes in the pneumatic displacement group (38 received C3F8, 2 received SF6) and 23 (36.5%) eyes in the subretinal cocktail injection. Mean baseline VA was 1.46 and 1.62, respectively (p = 0.404). The subretinal injection group had more extensive SMH (p = 0.005), thicker CST (1,006.6 μm vs. 780.2 μm, p = 0.012), and longer interval between symptom and operation (10.65 vs. 5.53 days, p < 0.001). The mean postoperative VA at 6 months was 0.67 and 0.91 (p = 0.180) for pneumatic displacement and subretinal injection groups, respectively, though VA was significantly better in the pneumatic group at 12-month visit (0.64 vs. 1.03, p = 0.040). At least 10 mean change in VA were >10 letters gain in both groups up to 12 months. Postoperative CST reduction was greater (625.1 μm vs. 326.5 μm, p = 0.008) and complete foveal displacement (87.0% vs. 37.5%), p < 0.001, odds ratio [OR] = 11.1) and displacement to arcade or beyond (52.5% vs. 17.5%, p = 0.009, OR = 5.15) were more frequent in the subretinal injection group. Two patients with failed pneumatic displacement were successfully treated with subretinal cocktail injection as a second operation. Conclusion: Surgical displacement of SMH leads to clinically meaningful improvement in VA. PPV with subretinal cocktail injection is more effective than pneumatic displacement in displacing SMH with similar safety profile despite longer interval before operation, higher CST, and more extensive SMH at baseline. Retinal surgeons could consider this novel technique in cases with thick and extensive SMH or as a rescue secondary operation in selected cases.
Hepatocellular Adenoma: A Life-Threatening Presentation of a Rare Liver Tumor – Case Report and Literature Review
Authors: Ramos Lopes Sara , Santos Inês Costa , Teixeira Madalena , Sequeira Cristiana , Carvalho Ana Maria , Gamito Élia
Published: April 2024

Hepatocellular adenoma is a rare benign liver tumor that occurs predominantly in young women who use exogenous estrogens. We describe a case of a 40-year-old woman on birth control pill who presented with acute right hypochondrial pain and signs of hemodynamical instability triggered by a large bleeding tumor in the right liver lobe. Arterial embolization was performed with cessation of bleeding. To determine etiology, magnetic resonance imaging was conducted with findings suggestive of a hepatocellular adenoma. The tumor was surgically resected, and histologic examination made the definite diagnosis of an inflammatory hepatocellular adenoma. This is a noteworthy case of a rare but potentially fatal complication of liver tumors, whose diagnosis requires a high index of suspicion. Clinicians should consider this diagnosis in young women on oral contraceptive pills who present with severe unexplained abdominal pain and hemodynamical instability. This case aimed to raise awareness of this condition, while reviewing important aspects concerning the management of hepatocellular adenomas.
Survival from rhino-orbital-cerebral mucormycosis in SARS-COV-2 positive diabetic patients: Two case reports
Authors: Lädrach Claudia , Wartenberg Martin , Zimmerli Stefan , Anschütz Lukas , Bohlen Stefan , Ebner Julian , de Gouyon Matignon de Pontouraude Claire M. F. , Caversaccio Marco , Wagner Franca
Published: April 2024

Introduction
Rhino-orbital-cerebral mucormycosis (ROCM) is a rare angioinvasive fungal infection known to be associated with high morbidity and over 50% mortality. ROCM is becoming more common due to an increase in predisposing immunocompromising comorbidities as well as COVID-19.
Case Presentations
We report two cases – a 75-year-old woman with diabetes and a 39-year-old man with recurrent diabetic ketoacidosis. Both presented initially with acute sinonasal symptoms, were positive for SARS-CoV-2, and diagnosed with acute ROCM. Both underwent mutilating surgical therapy as well as high-dose amphotericin B treatment. With continued oral antifungal treatment, Patient 1 showed stable symptoms despite radiographically increasing disease and died of urosepsis 5 months after first surgery. With posaconazole treatment, Patient 2 recovered from the disease and showed no clinical sign of disease progression after 1 year.
Conclusion
Despite the rarity of the disease, ROCM should be considered if the findings of clinical and radiological examination fit, so that a delay in treatment initiation can be avoided. As our both cases show, survival from ROCM is possible – albeit at a high cost.
POLA NAFKAH PETANI SAWIT UNTUK MEMPERTAHANKAN KEBERLANJUTAN EKONOMI RUMAH TANGGA DI DESA JENGGALU, BENGKULU
Authors: Irawan Andi , Restianingrum Lika
Published: April 2024

Penelitian ini menganalisis aset nafkah, pendapatan, dan pola nafkah rumah tangga petani sawit di Desa Jenggalu, Kecamatan Sukaraja, Kabupaten Bengkulu dalam rangka mempertahankan keberlanjutan ekonomi rumah tangga mereka. 40 petani dianalisis secara acak dari populasi 216 petani. Data dianalisis secara deskriptif dengan bantuan Excel. Mayoritas petani memiliki lahan sempit (67,5%) dengan aset finansial yang baik (52,5%). Pendapatan petani dari usaha tani sawit adalah Rp 37.819.690,54 per hektar per tahun dan mencukupi untuk memenuhi kebutuhan pokok rumah tangga mereka. Pola nafkah yang ditemukan adalah; 1) diversifikasi on-farm (sawit dan padi, sawit dan karet, sawit dan buruh tani); dan 2) diversifikasi non-farm yakni sawit dan dagang, sawit dan kuli, sawit dan pegawai, serta sawit dan pekerja harian lepas.
A liberdade é mais importante que o pão
Authors: Góes Moacyr
Published: April 2024

Depoimento de Moacyr Góes.
Uma visita ao abismo: entrevista com Marco Antonio Braz
Authors: Hamann Fernanda
Published: April 2024

Entrevista com Marco Antonio Braz.
O dramaturgo Nelson Rodrigues: conservador e revolucionário
Authors: Aguiar Flávio
Published: April 2024

O presente ensaio defende a tese de que os ideais conservadores do ponto de vista político e social, mantidos por Nelson Rodrigues, abriram-lhe o caminho para a criação de uma estética teatral revolucionária na peça Vestido de Noiva. Em apoio a ela examina outros casos semelhantes na dramaturgia brasileira, os de José de Anchieta e de José de Alencar. Apoia-se na análise da construção formal do enredo da peça, dissolvendo-lhe a linearidade comum na dramaturgia da época.
MFAP4-Deficiency Aggravates Age-Induced Changes in Resistance Artery Structure, While Ameliorating Hypertension
Authors: Christensen Kimmie B. , Ünsal Şeyda , Ebbesen Morten F. , Hemstra Line , Schlosser Anders , Rosenstand Kristoffer , Hansen Pernille B.L. , Jensen Boye L. , Bloksgaard Maria , Simonsen Ulf , Sorensen Grith L.
Published: April 2024

BACKGROUND:

Abnormalities of resistance arteries may play essential roles in the pathophysiology of aging and hypertension. Deficiency of the vascular extracellular matrix protein MFAP4 (microfibrillar-associated protein 4) has previously been observed as protective against aberrant arterial remodeling. We hypothesized that MFAP4-deficiency would reduce age- and hypertension-dependent arterial changes in extracellular matrix composition and stiffening.

METHODS:

Mesenteric arteries were isolated from old (20–23 months) littermate
Mfap4

+/+

and
Mfap4

−/−

mice, and 2-photon excitation microscopy imaging was used to quantify elastin and collagen volumes and dimensions in the vascular wall.

Ten-week-old littermate
Mfap4

+/+

and
Mfap4

−/−

mice were subjected to 20 days of continuous Ang II (angiotensin II) infusion and hypertension was monitored using invasive blood pressure measurements. Arterial stiffness, responses to vascular constrictors, and myogenic tone were monitored using wire- or pressure-myography. Collagen contents were assessed by Western blotting.

RESULTS:

MFAP4-deficiency significantly increased collagen volume and elastin fragmentation in aged mesenteric arteries without affecting arterial stiffness. MFAP4-deficient mice exhibited reduced diastolic pressure in Ang II-induced hypertension. There was no significant effect of MFAP4-deficiency on mesenteric artery structural remodeling or myogenic tone, although collagen content in mesenteric arteries was tendentially increased in hypertensive
Mfap4

+/+

mice relative to
Mfap4

−/−

mice. Increased efficacy of vasoconstrictors (phenylephrine, thromboxane) and reduced stiffness were observed in Ang II-treated
Mfap4

−/−

mouse mesenteric arteries in ex vivo myography recordings.

CONCLUSIONS:

MFAP4-deficiency reduces the elastin/collagen ratio in the aging resistance artery without affecting arterial stiffness. In contrast, MFAP4-deficiency reduces the stiffness of resistance arteries and ameliorates Ang II-induced hypertension.
Descriptions of two new species of Bactrocera Macquart (Diptera: Tephritidae: Dacinae: Dacini) from the Philippines with distinct orange medial stripes on the scutum
Authors: KORNEYEV SEVERYN V. , LEBLANC LUC , HAUSER MARTIN , GENERAL DAVID E. M. , GAIMARI STEPHEN D.
Published: April 2024

Two previously undescribed species of Bactrocera Macquart with distinct orange medial stripes on the scutum were collected in multi-lure traps baited with cue-lure during a fruit fly survey carried out in the Philippines in 2019. We describe Bactrocera youngi sp. n. and B. ellenriederae sp. n., both in the subgenus Bactrocera. An illustrated identification key is given for all known species of Bactrocera with orange stripes on the scutum. COI mitochondrial DNA sequences confirm that our new species are distinct from the other species with orange stripes. Additionally, B. bivittata Lin & Wang, 2005 is recorded from Thailand for the first time.
Factor Identification for the Procurement of Raw Material in Food Processing Industry
Authors: Dua Aman , Sahu Sanjay
Published: April 2024

Procuring raw materials (RM) is essential in the supply chain (SC), particularly in the current business landscape where organisations actively seek innovative ideas to cut costs and maintain competitiveness. The procurement operation enables these organisations to navigate through increasing cost pressures. Within SC logistics, the procurement of RM faces numerous obstacles and complexities. To address these challenges and propose potential solutions, examining the specific issues that impact the procurement of RM in the Food Processing Industry (FPI) is necessary. Therefore, this article explores the challenges affecting the procurement process. The authors also provided recommendations for mitigating the identified challenges. This study utilises interpretive structural model methodology, a qualitative and interpretive approach that produces solutions for complicated issues through discussions based on the structural mapping of intricate relationships between elements to look at and analyse the challenges that affect the procurement process. A systematic review of the existing literature provides a comprehensive understanding of these challenges. The study found that challenges in contract and procurement, infrastructure and transportation, technology, govt regulations, quality and safety standards, etc., are the factors that constitute severe challenges and affect the growth of FPI. Data were collected from only those working in Indian companies or multinational companies operating in India. Every step of the process, starting from the development of the questionnaire to the data collection itself, is carried out with a focus on the Indian perspective. Addressing these problems and obstacles will yield advantages for decision-makers and stakeholders, including farmers, manufacturers, customers and consumer Food Business Organisations. It will enhance their comprehension of the current situation, identify issues and confront challenges, thereby facilitating improved planning and management strategies.
The impact of cytotoxic therapy on the risk of progression and death in clonal cytopenia(s) of undetermined significance
Authors: Li Marissa , Baranwal Anmol , Gurney Mark , Shah Syed N. , Al-Kali Aref , Alkhateeb Hassan , Foran James , Arana Yi Cecilia , Ongie Laura , Chen Dong , Mangaonkar Abhishek , McCullough Kristen , Tefferi Ayalew , Lasho Terra , Finke Christy , Patnaik Mrinal M. , Shah Mithun Vinod
Published: April 2024

Clonal cytopenia of undetermined significance (CCUS) is defined by a myeloid driver mutation in the context of otherwise unexplained cytopenia. CCUS has an inherent risk of progressing to myeloid neoplasm. However, it is unknown how exposure to previous cytotoxic therapy may impact the risk of progression and survival. We stratified patients with CCUS by prior exposure to DNA-damaging therapy. Of 151 patients, 46 (30%) had received cytotoxic therapy and were classified as therapy-related CCUS (t-CCUS), whereas 105 (70%) had de novo CCUS. A lower proportion of t-CCUS had hypercellular marrows (17.8% vs 44.8%, P = .002) but had higher median bone marrow blast percentages. After a median follow-up of 2.2 years, t-CCUS had significantly shorter progression-free survival (PFS, 1.8 vs 6.3 years; hazard ratio [HR], 2.1; P = .007) and median overall survival (OS; 3.6 years vs not reached; HR, 2.3; P = .007) compared with CCUS. Univariable and multivariable time-to-event analyses showed that exposure to cytotoxic therapy independently accounted for inferior PFS and OS. Despite the similarities in clinical presentation between CCUS and t-CCUS, we show that exposure to prior cytotoxic therapies was an independent risk factor for inferior outcomes. This suggests that t-CCUS represents a unique clinical entity that needs more stringent monitoring or earlier intervention strategies.
Elucidating the ecophysiology of soybean pod-sucking stinkbug Riptortus pedestris (Hemiptera: Alydidae) based on de novo genome assembly and transcriptome analysis
Authors: Li Chade , Nong Wenyan , Boncan Delbert Almerick T. , So Wai Lok , Yip Ho Yin , Swale Thomas , Jia Qi , Vicentin Ignacio G. , Chung Gyuhwa , Bendena William G. , Ngo Jacky C. K. , Chan Ting Fung , Lam Hon-Ming , Hui Jerome H. L.
Published: April 2024

Food security is important for the ever-growing global population. Soybean, Glycine max (L.) Merr., is cultivated worldwide providing a key source of food, protein and oil. Hence, it is imperative to maintain or to increase its yield under different conditions including challenges caused by abiotic and biotic stresses. In recent years, the soybean pod-sucking stinkbug Riptortus pedestris has emerged as an important agricultural insect pest in East, South and Southeast Asia. Here, we present a genomics resource for R. pedestris including its genome assembly, messenger RNA (mRNA) and microRNA (miRNA) transcriptomes at different developmental stages and from different organs. As insect hormone biosynthesis genes (genes involved in metamorphosis) and their regulators such as miRNAs are potential targets for pest control, we analyzed the sesquiterpenoid (juvenile) and ecdysteroid (molting) hormone biosynthesis pathway genes including their miRNAs and relevant neuropeptides. Temporal gene expression changes of these insect hormone biosynthesis pathways were observed at different developmental stages. Similarly, a diet-specific response in gene expression was also observed in both head and salivary glands. Furthermore, we observed that microRNAs (bantam, miR-14, miR-316, and miR-263) of R. pedestris fed with different types of soybeans were differentially expressed in the salivary glands indicating a diet-specific response. Interestingly, the opposite arms of miR-281 (-5p and -3p), a miRNA involved in regulating development, were predicted to target Hmgs genes of R. pedestris and soybean, respectively. These observations among others highlight stinkbug’s responses as a function of its interaction with soybean. In brief, the results of this study not only present salient findings that could be of potential use in pest management and mitigation but also provide an invaluable resource for R. pedestris as an insect model to facilitate studies on plant-pest interactions.
Staphylococcus aureus foldase PrsA contributes to the folding and secretion of protein A
Authors: Lin Mei-Hui , Liu Chao-Chin , Lu Chiao-Wen , Shu Jwu-Ching
Published: April 2024

Background

Staphylococcus aureus secretes a variety of proteins including virulence factors that cause diseases. PrsA, encoded by many Gram-positive bacteria, is a membrane-anchored lipoprotein that functions as a foldase to assist in post-translocational folding and helps maintain the stability of secreted proteins. Our earlier proteomic studies found that PrsA is required for the secretion of protein A, an immunoglobulin-binding protein that contributes to host immune evasion. This study aims to investigate how PrsA influences protein A secretion.

Results

We found that in comparison with the parental strain HG001, the prsA-deletion mutant HG001ΔprsA secreted less protein A. Deleting prsA also decreased the stability of exported protein A. Pulldown assays indicated that PrsA interacts with protein A in vivo. The domains in PrsA that interact with protein A are mapped to both the N- and C-terminal regions (NC domains). Additionally, the NC domains are essential for promoting PrsA dimerization. Furthermore, an immunoglobulin-binding assay revealed that, compared to the parental strain HG001, fewer immunoglobulins bound to the surface of the mutant strain HG001ΔprsA.

Conclusions

This study demonstrates that PrsA is critical for the folding and secretion of protein A. The information derived from this study provides a better understanding of virulent protein export pathways that are crucial to the pathogenicity of S. aureus.
Sedation versus general anesthesia on all-cause mortality in patients undergoing percutaneous procedures: a systematic review and meta-analysis
Authors: Su Xuesen , Zhao Zixin , Zhang Wenjie , Tian Yihe , Wang Xin , Yuan Xin , Tian Shouyuan
Published: April 2024

Background

The comparison between sedation and general anesthesia (GA) in terms of all-cause mortality remains a subject of ongoing debate. The primary objective of our study was to investigate the impact of GA and sedation on all-cause mortality in order to provide clarity on this controversial topic.

Methods

A systematic review and meta-analysis were conducted, incorporating cohort studies and RCTs about postoperative all-cause mortality. Comprehensive searches were performed in the PubMed, EMBASE, and Cochrane Library databases, with the search period extending until February 28, 2023. Two independent reviewers extracted the relevant information, including the number of deaths, survivals, and risk effect values at various time points following surgery, and these data were subsequently pooled and analyzed using a random effects model.

Results

A total of 58 studies were included in the analysis, with a majority focusing on endovascular surgery. The findings of our analysis indicated that, overall, and in most subgroup analyses, sedation exhibited superiority over GA in terms of in-hospital and 30-day mortality. However, no significant difference was observed in subgroup analyses specific to cerebrovascular surgery. About 90-day mortality, the majority of studies centered around cerebrovascular surgery. Although the overall pooled results showed a difference between sedation and GA, no distinction was observed between the pooled ORs and the subgroup analyses based on RCTs and matched cohort studies. For one-year all-cause mortality, all included studies focused on cardiac and macrovascular surgery. No difference was found between the HRs and the results derived from RCTs and matched cohort studies.

Conclusions

The results suggested a potential superiority of sedation over GA, particularly in the context of cardiac and macrovascular surgery, mitigating the risk of in-hospital and 30-day death. However, for the longer postoperative periods, this difference remains uncertain.

Trial registration

PROSPERO CRD42023399151; registered 24 February 2023.