bluuebunny/crossref_metadata_2025 huggingface dataset:
Fish, Fish Oils and Cardioprotection: Promise or Fish Tale?
Authors: Goel Akshay , Pothineni Naga Venkata , Singhal Mayank , Paydak Hakan , Saldeen Tom , Mehta Jawahar L.
Published: November 2018

Fish and commercially available fish oil preparations are rich sources of long-chain omega-3 polyunsaturated fatty acids. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are the most important fatty acids in fish oil. Following dietary intake, these fatty acids get incorporated into the cell membrane phospholipids throughout the body, especially in the heart and brain. They play an important role in early brain development during infancy, and have also been shown to be of benefit in dementia, depression, and other neuropsychiatric disorders. Early epidemiologic studies show an inverse relationship between fish consumption and the risk of coronary heart disease. This led to the identification of the cardioprotective role of these marine-derived fatty acids. Many experimental studies and some clinical trials have documented the benefits of fish oil supplementation in decreasing the incidence and progression of atherosclerosis, myocardial infarction, heart failure, arrhythmias, and stroke. Possible mechanisms include reduction in triglycerides, alteration in membrane fluidity, modulation of cardiac ion channels, and anti-inflammatory, anti-thrombotic, and anti-arrhythmic effects. Fish oil supplements are generally safe, and the risk of toxicity with methylmercury, an environmental toxin found in fish, is minimal. Current guidelines recommend the consumption of either one to two servings of oily fish per week or daily fish oil supplements (around 1 g of omega-3 polyunsaturated fatty acids per day) in adults. However, recent large-scale studies have failed to demonstrate any benefit of fish oil supplements on cardiovascular outcomes and mortality. Here, we review the different trials that evaluated the role of fish oil in cardiovascular diseases.
Mouse Cardiac Pde1C Is a Direct Transcriptional Target of Pparα
Authors: Shete Varsha , Liu Ning , Jia Yuzhi , Viswakarma Navin , Reddy Janardan K. , Thimmapaya Bayar
Published: November 2018

Phosphodiesterase 1C (PDE1C) is expressed in mammalian heart and regulates cardiac functions by controlling levels of second messenger cyclic AMP and cyclic GMP (cAMP and cGMP, respectively). However, molecular mechanisms of cardiac Pde1c regulation are currently unknown. In this study, we demonstrate that treatment of wild type mice and H9c2 myoblasts with Wy-14,643, a potent ligand of nuclear receptor peroxisome-proliferator activated receptor alpha (PPARα), leads to elevated cardiac Pde1C mRNA and cardiac PDE1C protein, which correlate with reduced levels of cAMP. Furthermore, using mice lacking either Pparα or cardiomyocyte-specific Med1, the major subunit of Mediator complex, we show that Wy-14,643-mediated Pde1C induction fails to occur in the absence of Pparα and Med1 in the heart. Finally, using chromatin immunoprecipitation assays we demonstrate that PPARα binds to the upstream Pde1C promoter sequence on two sites, one of which is a palindrome sequence (agcTAGGttatcttaacctagc) that shows a robust binding. Based on these observations, we conclude that cardiac Pde1C is a direct transcriptional target of PPARα and that Med1 may be required for the PPARα mediated transcriptional activation of cardiac Pde1C.
Aberrant Epigenetic Regulation in Head and Neck Cancer Due to Distinct EZH2 Overexpression and DNA Hypermethylation
Authors: Mochizuki Daiki , Misawa Yuki , Kawasaki Hideya , Imai Atsushi , Endo Shiori , Mima Masato , Yamada Satoshi , Nakagawa Takuya , Kanazawa Takeharu , Misawa Kiyoshi
Published: November 2018

Enhancer of Zeste homologue 2 (EZH2) overexpression is associated with tumor proliferation, metastasis, and poor prognosis. Targeting and inhibition of EZH2 is a potentially effective therapeutic strategy for head and neck squamous cell carcinoma (HNSCC). We analyzed EZH2 mRNA expression in a well-characterized dataset of 230 (110 original and 120 validation cohorts) human head and neck cancer samples. This study aimed to investigate the effects of inhibiting EZH2, either via RNA interference or via pharmacotherapy, on HNSCC growth. EZH2 upregulation was significantly correlated with recurrence (p < 0.001) and the methylation index of tumor suppressor genes (p < 0.05). DNMT3A was significantly upregulated upon EZH2 upregulation (p = 0.043). Univariate analysis revealed that EZH2 upregulation was associated with poor disease-free survival (log-rank test, p < 0.001). In multivariate analysis, EZH2 upregulation was evaluated as a significant independent prognostic factor of disease-free survival (hazard ratio: 2.085, 95% confidence interval: 1.390–3.127; p < 0.001). Cells treated with RNA interference and DZNep, an EZH2 inhibitor, showed the most dramatic changes in expression, accompanied with a reduction in the growth and survival of FaDu cells. These findings suggest that EZH2 upregulation is correlated with tumor aggressiveness and adverse patient outcomes in HNSCC. Evaluation of EZH2 expression might help predict the prognosis of HNSCC patients.
Matriptase-Induced Phosphorylation of MET is Significantly Associated with Poor Prognosis in Invasive Bladder Cancer; an Immunohistochemical Analysis
Authors: Yamasaki Koji , Mukai Shoichiro , Nagai Takahiro , Nakahara Kozue , Fujii Masato , Terada Naoki , Ohno Akinobu , Sato Yuichiro , Toda Yoshinobu , Kataoka Hiroaki , Kamoto Toshiyuki
Published: November 2018

Hepatocyte growth factor (HGF) plays an important role in cancer progression via phosphorylation of MET (c-met proto-oncogene product, receptor of HGF). HGF-zymogen (pro-HGF) must be processed for activation by HGF activators including matriptase, which is a type II transmembrane serine protease and the most efficient activator. The enzymatic activity is tightly regulated by HGF activator inhibitors (HAIs). Dysregulated pro-HGF activation (with upregulated MET phosphorylation) is reported to promote cancer progression in various cancers. We retrospectively analyzed the expression of matriptase, phosphorylated-MET (phospho-MET) and HAI-1 in tumor specimens obtained from patients with invasive bladder cancer by immunohistochemistry. High expression of phospho-MET and increased expression of matriptase were significantly associated with poor prognosis, and high matriptase/low HAI-1 expression showed poorer prognosis. Furthermore, high expression of matriptase tended to correlate with phosphorylation of MET. Increased expression of matriptase may induce the ligand-dependent activation of MET, which leads to poor prognosis in patients with invasive bladder cancer.
Prediction of Signal Peptides in Proteins from Malaria Parasites
Authors: Burdukiewicz Michał , Sobczyk Piotr , Chilimoniuk Jarosław , Gagat Przemysław , Mackiewicz Paweł
Published: November 2018

Signal peptides are N-terminal presequences responsible for targeting proteins to the endomembrane system, and subsequent subcellular or extracellular compartments, and consequently condition their proper function. The significance of signal peptides stimulates development of new computational methods for their detection. These methods employ learning systems trained on datasets comprising signal peptides from different types of proteins and taxonomic groups. As a result, the accuracy of predictions are high in the case of signal peptides that are well-represented in databases, but might be low in other, atypical cases. Such atypical signal peptides are present in proteins found in apicomplexan parasites, causative agents of malaria and toxoplasmosis. Apicomplexan proteins have a unique amino acid composition due to their AT-biased genomes. Therefore, we designed a new, more flexible and universal probabilistic model for recognition of atypical eukaryotic signal peptides. Our approach called signalHsmm includes knowledge about the structure of signal peptides and physicochemical properties of amino acids. It is able to recognize signal peptides from the malaria parasites and related species more accurately than popular programs. Moreover, it is still universal enough to provide prediction of other signal peptides on par with the best preforming predictors.
Actin Bundles in The Pollen Tube
Authors: Zhang Shujuan , Wang Chunbo , Xie Min , Liu Jinyu , Kong Zhe , Su Hui
Published: November 2018

The angiosperm pollen tube delivers two sperm cells into the embryo sac through a unique growth strategy, named tip growth, to accomplish fertilization. A great deal of experiments have demonstrated that actin bundles play a pivotal role in pollen tube tip growth. There are two distinct actin bundle populations in pollen tubes: the long, rather thick actin bundles in the shank and the short, highly dynamic bundles near the apex. With the development of imaging techniques over the last decade, great breakthroughs have been made in understanding the function of actin bundles in pollen tubes, especially short subapical actin bundles. Here, we tried to draw an overall picture of the architecture, functions and underlying regulation mechanism of actin bundles in plant pollen tubes.
The Impact of miRNA in Colorectal Cancer Progression and Its Liver Metastases
Authors: Balacescu Ovidiu , Sur Daniel , Cainap Calin , Visan Simona , Cruceriu Daniel , Manzat-Saplacan Roberta , Muresan Mihai-Stefan , Balacescu Loredana , Lisencu Cosmin , Irimie Alexandru
Published: November 2018

Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies with a high incidence and mortality rate. An essential challenge in colorectal cancer management is to identify new prognostic factors that could better estimate the evolution and treatment responses of this disease. Considering their role in cancer development, progression and metastasis, miRNAs have become an important class of molecules suitable for cancer biomarkers discovery. We performed a systematic search of studies investigating the role of miRNAs in colorectal progression and liver metastasis published until October 2018. In this review, we present up-to-date information regarding the specific microRNAs involved in CRC development, considering their roles in alteration of Wnt/βcatenin, EGFR, TGFβ and TP53 signaling pathways. We also emphasize the role of miRNAs in controlling the epithelial–mesenchymal transition of CRC cells, a process responsible for liver metastasis in a circulating tumor cell-dependent manner. Furthermore, we discuss the role of miRNAs transported by CRC-derived exosomes in mediating liver metastases, by preparing the secondary pre-metastatic niche and in inducing liver carcinogenesis in a Dicer-dependent manner.
Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line
Authors: Tyurin-Kuzmin Pyotr , Chechekhin Vadim , Ivanova Anastasiya , Dyikanov Daniyar , Sysoeva Veronika , Kalinina Natalia , Tkachuk Vsevolod
Published: November 2018

Primary adipose tissue-derived multipotent stem/stromal cells (adMSCs) demonstrate unusual signaling regulatory mechanisms, i.e., increased of sensitivity to catecholamines in response to noradrenaline. This phenomenon is called “heterologous sensitization”, and was previously found only in embryonic cells. Since further elucidation of the molecular mechanisms that are responsible for such sensitization in primary adMSCs was difficult due to the high heterogeneity in adrenergic receptor expression, we employed immortalized adipose-derived mesenchymal stem cell lines (hTERT-MSCs). Using flow cytometry and immunofluorescence microscopy, we demonstrated that the proportion of cells expressing adrenergic receptor isoforms does not differ significantly in hTERT-MSCs cells compared to the primary adMSCs culture. However, using analysis of Ca2+-mobilization in single cells, we found that these cells did not demonstrate the sensitization seen in primary adMSCs. Consistently, these cells did not activate cAMP synthesis in response to noradrenaline. These data indicate that immortalized adipose-derived mesenchymal stem cell lines demonstrated impaired ability to respond to noradrenaline compared to primary adMSCs. These data draw attention to the usage of immortalized cells for MSCs-based regenerative medicine, especially in the field of pharmacology.
Ultrasound Imaging for the Cutaneous Nerves of the Extremities and Relevant Entrapment Syndromes: From Anatomy to Clinical Implications
Authors: Chang Ke-Vin , Mezian Kamal , Naňka Ondřej , Wu Wei-Ting , Lou Yueh-Ming , Wang Jia-Chi , Martinoli Carlo , Özçakar Levent
Published: November 2018

Cutaneous nerve entrapment plays an important role in neuropathic pain syndrome. Due to the advancement of ultrasound technology, the cutaneous nerves can be visualized by high-resolution ultrasound. As the cutaneous nerves course superficially in the subcutaneous layer, they are vulnerable to entrapment or collateral damage in traumatic insults. Scanning of the cutaneous nerves is challenging due to fewer anatomic landmarks for referencing. Therefore, the aim of the present article is to summarize the anatomy of the limb cutaneous nerves, to elaborate the scanning techniques, and also to discuss the clinical implications of pertinent entrapment syndromes of the medial brachial cutaneous nerve, intercostobrachial cutaneous nerve, medial antebrachial cutaneous nerve, lateral antebrachial cutaneous nerve, posterior antebrachial cutaneous nerve, superficial branch of the radial nerve, dorsal cutaneous branch of the ulnar nerve, palmar cutaneous branch of the median nerve, anterior femoral cutaneous nerve, posterior femoral cutaneous nerve, lateral femoral cutaneous nerve, sural nerve, and saphenous nerve.
Anatomic Association of the Proximal Fingernail Matrix to the Extensor Pollicis Longus Tendon: A Morphological and Histological Study
Authors: Palomo-López Patricia , Becerro-de-Bengoa-Vallejo Ricardo , López-López Daniel , Calvo-Lobo César , Herrera-Lara Manuel , Murillo-González Jorge Alfonso , Losa-Iglesias Marta Elena
Published: November 2018

Background: Extensor tendon disorders may cause severe functional impairments, and there is a lack of knowledge about their anatomic associations with the proximal fingernail matrix. Objective: To delineate the association between the distal extensor pollicis longus tendon (EPLT) insertion and the limit of the fingernail matrix in the thumb. Methods: The limit of the fingernail matrix and the distal bony insertion of the EPLT were identified in five thumbs from fresh-frozen human cadavers. An additional five thumbs were fixed and the longitudinal thumb sections were histologically analyzed. Results: The terminal limit of the matrix and fingernail was dorsal and overlapped to the EPL tendon, which was located between the fingernail matrix and the phalanx, and extended dorsally to the distal section of the terminal phalanx in all ten thumb bodies. Conclusion: The fingernail matrix is not directly inserted into the periosteum of the dorsal section of the base to the distal phalanx, because this anatomic relationship is separated by the deep fibers of the EPLT.
An Improved Multi-Relaxation Time Lattice Boltzmann Method for the Non-Newtonian Influence of the Yielding Fluid Flow in Cement-3D Printing
Authors: Huang Tiancheng , Gu Hai , Zhang Jie , Li Bin , Sun Jianhua , Wu Weiwei
Published: November 2018

The multi-relaxation time lattice Boltzmann method (MRT-LBM) has an excellent performance in dealing with the complex flow in many different areas. According to the specific behavior of the fluids, it also has some shortcomings when applied to some special flow like as the non-Newtonian flow. In Cement-3D printing, the fluids always exhibit according to the yielding behavior. When using the standard MRT-LBM, the simulation maybe divergent. In order to solve the problem, this work presents an improved MRT-LBM considering the non-Newtonian effect as a special forcing term to ensure the stable and accurate simulation. Finally, the Poiseuille flow was used to validate the feasibility of the proposed method.
Effect of Selective Laser Melting Process Parameters on the Quality of Al Alloy Parts: Powder Characterization, Density, Surface Roughness, and Dimensional Accuracy
Authors: Maamoun Ahmed H. , Xue Yi F. , Elbestawi Mohamed A. , Veldhuis Stephen C.
Published: November 2018

Additive manufacturing (AM) of high-strength Al alloys promises to enhance the performance of critical components related to various aerospace and automotive applications. The key advantage of AM is its ability to generate lightweight, robust, and complex shapes. However, the characteristics of the as-built parts may represent an obstacle to the satisfaction of the parts’ quality requirements. The current study investigates the influence of selective laser melting (SLM) process parameters on the quality of parts fabricated from different Al alloys. A design of experiment (DOE) was used to analyze relative density, porosity, surface roughness, and dimensional accuracy according to the interaction effect between the SLM process parameters. The results show a range of energy densities and SLM process parameters for AlSi10Mg and Al6061 alloys needed to achieve “optimum” values for each performance characteristic. A process map was developed for each material by combining the optimized range of SLM process parameters for each characteristic to ensure good quality of the as-built parts. This study is also aimed at reducing the amount of post-processing needed according to the optimal processing window detected.
Comparison of a Novel Ultrasonic Scaler Tip vs. Conventional Design on a Titanium Surface
Authors: Sinjari Bruna , D’Addazio Gianmaria , Bozzi Martina , Celletti Renato , Traini Tonino , Mavriqi Luan , Caputi Sergio
Published: November 2018

The aim of this in vitro study was to evaluate the alterations of a titanium surface after treatment with two different types of ultrasonic tips: conventional steel versus an innovative copper alloy silver-plated one. Twenty smooth-surface, grade IV unalloyed titanium discs were divided into two groups. The discs were ultrasonically instrumented and the scaler was connected with a loading machine. The surface morphology was examined using scanning electron microscopy (SEM) and fractal analysis of lacunarity was calculated to highlight the alteration of the surface using the two different tips. The SEM analysis showed different degrees of surface roughness between the two types of scaler tips. Moreover, these observations demonstrated that the new tip showed fewer irregularities on the disc’s surface than the conventional steel tip. The statistical and fractal analysis showed a statistically significant difference between the two groups. Surface alterations of titanium induced by the conventional ultrasonic tips were much greater than those made by copper alloy silver plated tips. The presented results suggest that the use of this new ultrasonic tip may reduce the alterations on the implant surface during its use in dental practice.
Theoretical Study of Hydrogen on LaFeO3 (010) Surface Adsorption and Subsurface Diffusion
Authors: Pan Changchang , Chen Yuhong , Zhang Meiling , Yuan Lihua , Zhang Cairong
Published: November 2018

Based on density functional theory, this paper studies the adsorption and the subsurface occupation by H on LaFeO3 (010) surface and their corresponding transition states. As shown from the results, the best storage positions of hydrogen are on the O top position of the LaFeO3 (010) surface and the interstice near the oxygen of the subsurface. In addition, the position of surface Fe atom can also store hydrogen, but H atom prefers to adsorb on O atom first. Whether the H atom is adsorbed on O or Fe atom, it is easy diffuse to the nearby more stable O atom. However, the diffusion between the Fe atoms is difficult to occur. The main diffusion path of the H atom from the surface to the subsurface is the process of inward layer by layer around the O atom. With the fracture of the old H–O bond and the formation of the new H–O bond, the H is around O atom to constantly repeat the process of a hopping-rotational diffusion. H diffuses through the nearest neighbor position, which is more favorable than the direct diffusion.
Rheological and Curing Behavior of Acrylate-Based Suspensions for the DLP 3D Printing of Complex Zirconia Parts
Authors: Komissarenko Dmitrii A. , Sokolov Petr S. , Evstigneeva Anastasiya D. , Shmeleva Irina A. , Dosovitsky Alexey E.
Published: November 2018

The present study demonstrates the possibility of fabricating zirconia parts with a complex shape and internal architecture using a low-cost stereolithography-based technique. One of the critical steps in ceramics stereolithography is the preparation of a photo-curable slurry with properties that fulfill specific requirements, such as having a low viscosity, high solids loading and appropriate curing characteristics. Slurries with different acrylic monomers and ceramic fillers were studied concerning their rheological and curing behavior. New formulations based on mono- and tri-functional acrylic monomers revealed the following excellent rheological properties: The viscosity of the mono-/tri-acrylate-based slurry with 75 wt.% of zirconia was 1.6 Pa·s at 30 s−1. Zirconia stabilized with 3 mol.% yttria was found to be more favorable than zirconia with 8 mol.% yttria for slurry preparation, because of its lower surface area and higher tapped density. It was shown that the cure depth of the suspensions was suitable for printing objects with a 50 µm layer thickness, good interlayers connection and surface finishing.
Residual Tensile Strength of the Multi-Impacted Scarf-Repaired Glass Fiber-Reinforced Polymer (GFRP) Composites
Authors: Kumari Punita , Wang Jihui , Saahil
Published: November 2018

The effect of multiple/repeated impacts on a repaired composite was investigated using a low-velocity impact test. The composite samples were fabricated through a vacuum resin infusion method (VARI) and repaired by a scarf repair technique. Later, a repeated low-velocity impact test was performed on the original and repaired composites samples. Performance of the multi-impacted repaired and original samples was evaluated and compared by measuring maximum contact force, maximum displacement, maximum time duration, absorbed energy and damage area. Photographs of the post-impacted samples were taken to observe the multi-impact damage progression through visual inspection. The results showed that each repeated impact subjected the samples to more damage. Tensile tests revealed that the scarf repair restored 81.23% strength. It was also observed that the sample obtained the highest damage dent in the low-velocity impact test that failed early during a tensile test and carried the lowest ultimate load.
Ni(II) Dimers of NNO Donor Tridentate Reduced Schiff Base Ligands as Alkali Metal Ion Capturing Agents: Syntheses, Crystal Structures and Magnetic Properties
Authors: Mondal Monotosh , Chakraborty Maharudra , Drew Michael G. B. , Ghosh Ashutosh
Published: November 2018

Three trinuclear Ni(II)-Na(I) complexes, [Ni2(L1)2NaCl3(H2O)]·H2O (1), [Ni2(L2)2NaCl3(H2O)] (2), and [Ni2(L3)2NaCl3(OC4H10)] (3) have been synthesized using three different NNO donor tridentate reduced Schiff base ligands, HL1 = 2-[(3-methylamino-propylamino)-methyl]-phenol, HL2 = 2-[(3-methylamino-propylamino)-methyl]-4-chloro-phenol, and HL3 = 2-[(3-methylamino-propylamino)-methyl]-6-methoxy-phenol that had been structurally characterized. Among these complexes, 1 and 2 are isostructural in which dinuclearNi(II) units act as metalloligands to bind Na(I) ions via phenoxido and chlorido bridges. The Na(I) atom is five-coordinated, and the Ni(II) atom possesses hexacordinated distorted octahedral geometry. In contrast, in complex 3, two -OMe groups from the dinuclear Ni(II) unit also coordinate to Na(I) to make its geometry heptacordinated pentagonal bipyramidal. The magnetic measurements of complexes 1–3 indicate ferromagnetic interactions between dimeric Ni(II) units with J = 3.97 cm−1, 4.66 cm−1, and 5.50 cm−1 for 1–3, respectively, as is expected from their low phenoxido bridging angles (89.32°, 89.39°, and 87.32° for 1–3, respectively). The J values have been calculated by broken symmetry DFT method and found to be in good agreement with the experimental values.
The Extremal Graphs of Some Topological Indices with Given Vertex k-Partiteness
Authors: Gao Fang , Li Xiaoxin , Zhou Kai , Liu Jia-Bao
Published: November 2018

The vertex k-partiteness of graph G is defined as the fewest number of vertices whose deletion from G yields a k-partite graph. In this paper, we characterize the extremal value of the reformulated first Zagreb index, the multiplicative-sum Zagreb index, the general Laplacian-energy-like invariant, the general zeroth-order Randić index, and the modified-Wiener index among graphs of order n with vertex k-partiteness not more than m .
Neuroprotective Activity of Some Marine Fungal Metabolites in the 6-Hydroxydopamin- and Paraquat-Induced Parkinson’s Disease Models
Authors: Yurchenko Ekaterina A. , Menchinskaya Ekaterina S. , Pislyagin Evgeny A. , Trinh Phan Thi Hoai , Ivanets Elena V. , Smetanina Olga F. , Yurchenko Anton N.
Published: November 2018

A new melatonin analogue 6-hydroxy-N-acetyl-β-oxotryptamine (1) was isolated from the marine-derived fungus Penicillium sp. KMM 4672. It is the second case of melatonin-related compounds isolation from microfilamentous fungi. The neuroprotective activities of this metabolite, as well as 3-methylorsellinic acid (2) and 8-methoxy-3,5-dimethylisochroman-6-ol (3) from Penicillium sp. KMM 4672, candidusin A (4) and 4″-dehydroxycandidusin A (5) from Aspergillus sp. KMM 4676, and diketopiperazine mactanamide (6) from Aspergillus flocculosus, were investigated in the 6-hydroxydopamine (6-OHDA)- and paraquat (PQ)-induced Parkinson’s disease (PD) cell models. All of them protected Neuro2a cells against the damaging influence of 6-OHDA to varying degrees. This effect may be realized via a reactive oxygen species (ROS) scavenging pathway. The new melatonin analogue more effectively protected Neuro2A cells against the 6-OHDA-induced neuronal death, in comparison with melatonin, as well as against the PQ-induced neurotoxicity. Dehydroxylation at C-3″ and C-4″ significantly increased free radical scavenging and neuroprotective activity of candidusin-related p-terphenyl polyketides in both the 6-OHDA- and PQ-induced PD models.
Oxysterols from a Marine Sponge Inflatella sp. and Their Action in 6-Hydroxydopamine-Induced Cell Model of Parkinson’s Disease
Authors: Kolesnikova Sophia A. , Lyakhova Ekaterina G. , Kalinovsky Anatoly I. , Popov Roman S. , Yurchenko Ekaterina A. , Stonik Valentin A.
Published: November 2018

Four new oxysterols 1–4 along with previously known oxygenated sterols 5–14 were isolated from the sponge Inflatella sp., collected from the Sea of Okhotsk. Structures of 1–4 were elucidated by the detailed NMR spectroscopic and mass-spectrometric analyses as well as by comparison of the corresponding experimental data with those reported in literature. The influence of compounds 1–14 on the viability of neuronal Neuro2a cells treated by 6-hydroxydopamine and reactive oxygen species (ROS) formation in these cells was investigated.
The Role of Zwitterionic Materials in the Fight against Proteins and Bacteria
Authors: Colilla Montserrat , Izquierdo-Barba Isabel , Vallet-Regí María
Published: November 2018

Zwitterionization of biomaterials has been heightened to a potent tool to develop biocompatible materials that are able to inhibit bacterial and non-specific proteins adhesion. This constitutes a major progress in the biomedical field. This manuscript overviews the main functionalization strategies that have been reported up to date to design and develop these advanced biomaterials. On this regard, the recent research efforts that were dedicated to provide their surface of zwitterionic nature are summarized by classifying biomaterials in two main groups. First, we centre on biomaterials in clinical use, concretely bioceramics, and metallic implants. Finally, we revise emerging nanostructured biomaterials, which are receiving growing attention due to their multifunctionality and versatility mainly in the local drug delivery and bone tissue regeneration scenarios.
Editorial for the Special Issue on 3D Printed Microfluidic Devices
Authors: Tasoglu Savas , Folch Albert
Published: November 2018

Three-dimensional (3D) printing has revolutionized the microfabrication prototyping workflow over the past few years. [...]
A Miniaturized Amperometric Hydrogen Sulfide Sensor Applicable for Bad Breath Monitoring
Authors: Gatty Hithesh K. , Stemme Göran , Roxhed Niclas
Published: November 2018

Bad breath or halitosis affects a majority of the population from time to time, causing personal discomfort and social embarrassment. Here, we report on a miniaturized, microelectromechanical systems (MEMS)-based, amperometric hydrogen sulfide (H2S) sensor that potentially allows bad breath quantification through a small handheld device. The sensor is designed to detect H2S gas in the order of parts-per-billion (ppb) and has a measured sensitivity of 0.65 nA/ppb with a response time of 21 s. The sensor was found to be selective to NO and NH3 gases, which are normally present in the oral breath of adults. The ppb-level detection capability of the integrated sensor, combined with its relatively fast response and high sensitivity to H2S, makes the sensor potentially applicable for oral breath monitoring.
In Vivo X-ray Computed Tomography Investigations of Crack Damage Evolution of Cemented Waste Rock Backfills (CWRB) under Uniaxial Deformation
Authors: Wang Yu , Li Changhong , Hou Zhiqiang , Yi Xuefeng , Wei Xiaoming
Published: November 2018

Cemented waste rock backfill (CWRB), which is a mixture of tailings, waste rock, cement, and water, is subjected to combination actions in underground mining operations and has been widely used in deep resource mining. While the strength requirement and macroscopic deformation behaviors of CWRB have been well studied, the mesoscopic damage evolution mechanisms are still not well understood. In this work, a CWRB sample with a waste rock proportion of 30% was studied with a uniaxial compression test under tomographic monitoring, using a 450 kV industrial X-ray computed tomography (CT). Clear CT images, CT value analysis, crack identification, and extraction reveal that CWRB damage evolution is extremely inhomogeneous and affected by the waste rock size, shape, and distribution. Furthermore, the crack initiation, propagation, and coalescence behaviors are limited to the existing waste rocks. When deformation grows to a certain extent, the cracks demonstrate an interlocking phenomenon and their propagation paths are affected by the waste rocks, which may improve the ability to resist compressive deformation. Volumetric dilatancy caused by the damage and cracking behavior has closed a link with the meso-structural changes, which are controlled by the interactions between the waste rocks and the cemented tailing paste.
Forecasting Geoenvironmental Risks: Integrated Applications of Mineralogical and Chemical Data
Authors: Parbhakar-Fox Anita , Fox Nathan , Jackson Laura , Cornelius Rebekah
Published: November 2018

Management of solid mine wastes requires detailed material characterisation at the start of a project to minimize opportunities for the generation of acid and metalliferous drainage (AMD). Mine planning must focus on obtaining a thorough understanding of the environmental properties of the future waste rock materials. Using drill core obtained from a porphyry Cu project in Northern Europe, this study demonstrates the integrated application of mineralogical and geochemical data to enable the construction of enviro-geometallurgical models. Geoenvironmental core logging, static chemical testing, bulk- and hyperspectral mineralogical techniques, and calculated mineralogy from assay techniques were used to critically evaluate the potential for AMD formation. These techniques provide value-adding opportunities to existing datasets and provide robust cross-validation methods for each technique. A new geoenvironmental logging code and a new geoenvironmental index using hyperspectral mineralogical data (Hy-GI) were developed and embedded into the geochemistry-mineralogy-texture-geometallurgy (GMTG) approach for waste characterisation. This approach is recommended for new mining projects (i.e., early life-of-mine stages) to ensure accurate geoenvironmental forecasting, therefore facilitating the development of an effective waste management plan that minimizes geoenvironmental risks posed by the mined materials.
A Review on Electroporation-Based Intracellular Delivery
Authors: Shi Junfeng , Ma Yifan , Zhu Jing , Chen Yuanxin , Sun Yating , Yao Yicheng , Yang Zhaogang , Xie Jing
Published: November 2018

Intracellular delivery is a critical step in biological discoveries and has been widely utilized in biomedical research. A variety of molecular tools have been developed for cell-based gene therapies, including FDA approved CAR-T immunotherapy, iPSC, cell reprogramming and gene editing. Despite the inspiring results of these applications, intracellular delivery of foreign molecules including nucleic acids and proteins remains challenging. Efficient yet non-invasive delivery of biomolecules in a high-throughput manner has thus long fascinates the scientific community. As one of the most popular non-viral technologies for cell transfection, electroporation has gone through enormous development with the assist of nanotechnology and microfabrication. Emergence of miniatured electroporation system brought up many merits over the weakness of traditional electroporation system, including precise dose control and high cell viability. These new generation of electroporation systems are of considerable importance to expand the biological applications of intracellular delivery, bypassing the potential safety issue of viral vectors. In this review, we will go over the recent progresses in the electroporation-based intracellular delivery and several potential applications of cutting-edge research on the miniatured electroporation, including gene therapy, cellular reprogramming and intracellular probe.
Lipoprotein Subclass Profile after Progressive Energy Deficits Induced by Calorie Restriction or Exercise
Authors: Chooi Yu Chung , Ding Cherlyn , Chan Zhiling , Lo Jezebel , Choo John , Ding Benjamin T. K. , Leow Melvin K.-S. , Magkos Faidon
Published: November 2018

Weight loss, induced by chronic energy deficit, improves the blood lipid profile. However, the effects of an acute negative energy balance and the comparative efficacy of diet and exercise are not well-established. We determined the effects of progressive, acute energy deficits (20% or 40% of daily energy requirements) induced by a single day of calorie restriction (n = 19) or aerobic exercise (n = 13) in healthy subjects (age: 26 ± 9 years; body mass index (BMI): 21.8 ± 2.9 kg/m2). Fasting plasma concentrations of very low-, intermediate-, low-, and high-density lipoprotein (VLDL, LDL, IDL, and HDL, respectively) particles and their subclasses were determined using nuclear magnetic resonance. Total plasma triglyceride and VLDL-triglyceride concentrations decreased after calorie restriction and exercise (all p ≤ 0.025); the pattern of change was linear with an increasing energy deficit (all p < 0.03), with no evidence of plateauing. The number of circulating large and medium VLDL particles decreased after diet and exercise (all p < 0.015), with no change in small VLDL particles. The concentrations of IDL, LDL, and HDL particles, their relative distributions, and the particle sizes were not altered. Our data indicate that an acute negative energy balance induced by calorie restriction and aerobic exercise reduces triglyceride concentrations in a dose-dependent manner, by decreasing circulating large and medium VLDL particles.
The Relationship between Body Composition and a Gluten Free Diet in Children with Celiac Disease
Authors: Więch Paweł , Chmiel Zdzisława , Bazaliński Dariusz , Sałacińska Izabela , Bartosiewicz Anna , Mazur Artur , Korczowski Bartosz , Binkowska-Bury Monika , Dąbrowski Mariusz
Published: November 2018

The primary and proven therapy, in cases of celiac disease (CD), is a rigorous gluten-free diet (GFD). However, there are reports of its negative effects in the form of nutritional deficiencies, obesity, and adverse changes in body composition. The study aimed to assess the impact of a GFD on the body composition of children with CD. In a case-controlled study (n = 41; mean age 10.81 y; SD = 3.96) children with CD, in various stages of treatment, underwent medical assessment. The control group consisted of healthy children and adolescents, strictly matched for gender and age in a 1:1 case-control manner. More than half of the examined children (n = 26) followed a GFD. CD children had significantly higher mean values of the fat free mass (FFM% = 80.68 vs. 76.66, p = 0.015), and total body water (TBW% = 65.22 vs. 60.47, p = 0.012), and lower mean values of the fat mass (FM% = 19.32 vs. 23.34, p = 0.015). Children who were on a GFD presented slightly higher, but not statistically significant, mean values of FM and FFM, than children who did not follow dietary recommendations (FM [kg] = 7.48 vs. 5.24, p = 0.064; FM% = 20.81 vs. 16.73, p = 0.087; FFM [kg] = 28.19 vs. 22.62, p = 0.110). After minimum one year of a GFD, CD children showed significantly higher values of FFM [kg] (p = 0.001), muscle mass (MM) [kg] (p < 0.001), TBW [L] (p < 0.001) and body cell mass (BCM) [kg] (p < 0.001). Furthermore, CD children who were on a GFD presented a significantly higher increase in weight (p = 0.034) and body mass index (BMI) (p = 0.021). The children adhering to a GFD demonstrate a tendency towards higher indices of selected body composition components.
A Randomized, Placebo-Controlled, Pilot Clinical Trial to Evaluate the Effect of Supplementation with Prebiotic Synergy 1 on Iron Homeostasis in Children and Adolescents with Celiac Disease Treated with a Gluten-Free Diet
Authors: Feruś Klaudia , Drabińska Natalia , Krupa-Kozak Urszula , Jarocka-Cyrta Elżbieta
Published: November 2018

Iron deficiency anemia (IDA) occurs in 15–46% of patients with celiac disease (CD), and in some cases, it may be its only manifestation. Studies in animal models have shown that prebiotics, including inulin, may help to increase intestinal absorption of iron. The aim of this study was to evaluate the effect of a prebiotic, oligofructose-enriched inulin (Synergy 1), on iron homeostasis in non-anemic children and adolescents with celiac disease (CD) in association with a gluten-free diet (GFD). Thirty-four CD patients (4–18 years old) were randomized into two groups receiving Synergy 1 (10 g/day) or a placebo (maltodextrin) for three months. Before and after intervention, blood samples were collected from all patients for assessment of blood morphology, biochemical parameters and serum hepcidin concentration. We found that serum hepcidin concentration after the intervention was significantly decreased by 60.9% (p = 0.046) in the Synergy 1 group, whereas no significant difference was observed in the placebo group. No differences in morphological and biochemical blood parameters (including ferritin, hemoglobin and C-reactive protein (CRP)) were observed after intervention in either group. Given that hepcidin decrease may improve intestinal iron absorption, these results warrant further investigation in a larger cohort and especially in patients with IDA.
Trace Element Provision in Parenteral Nutrition in Children: One Size Does Not Fit All
Authors: Zemrani Boutaina , McCallum Zoe , Bines Julie E
Published: November 2018

Routine administration of trace elements is recognised as a standard of care in children requiring parenteral nutrition. However, there is a lack of global consensus regarding trace elements provision and dosing in pediatric parenteral nutrition. This review provides an overview of available evidence regarding trace elements supply and posology in parenteral nutrition in neonates and children. Trace elements provision in children should be tailored to the weight and clinical condition of the child with emphasis on those at risk of toxicity or deficiency. Based on current evidence, there is a need to review the formulation of commercial solutions that contain multiple-trace elements and to enable individual trace elements additives to be available for specific indications. Literature supports the removal of chromium provision whereas manganese and molybdenum supplementation are debated. Preterm neonates may have higher parenteral requirements in iodine, selenium and copper than previously recommended. There is growing support for the routine provision of iron in long-term parenteral nutrition. Further studies on trace elements contamination of parenteral nutrition solutions are needed for a range of trace elements.
Calorie Restriction Mimetics: Upstream-Type Compounds for Modulating Glucose Metabolism
Authors: Shintani Hideya , Shintani Tomoya , Ashida Hisashi , Sato Masashi
Published: November 2018

Calorie restriction (CR) can prolong the human lifespan, but enforcing long-term CR is difficult. Therefore, a compound that reproduces the effect of CR without CR is needed. In this review, we summarize the current knowledge on compounds with CR mimetic (CRM) effects. More than 10 compounds have been listed as CRMs, some of which are conventionally categorized as upstream-type CRMs showing glycolytic inhibition, while the others are categorized as downstream-type CRMs that regulate or genetically modulate intracellular signaling proteins. Among these, we focus on upstream-type CRMs and propose their classification as compounds with energy metabolism inhibition effects, particularly glucose metabolism modulation effects. The upstream-type CRMs reviewed include chitosan, acarbose, sodium-glucose cotransporter 2 inhibitors, and hexose analogs such as 2-deoxy-d-glucose, d-glucosamine, and d-allulose, which show antiaging and longevity effects. Finally, we discuss the molecular definition of upstream-type CRMs.
Towards a Topological Representation of Risks and Their Measures
Authors: Shushi Tomer
Published: November 2018

In risk theory, risks are often modeled by risk measures which allow quantifying the risks and estimating their possible outcomes. Risk measures rely on measure theory, where the risks are assumed to be random variables with some distribution function. In this work, we derive a novel topological-based representation of risks. Using this representation, we show the differences between diversifiable and non-diversifiable. We show that topological risks should be modeled using two quantities, the risk measure that quantifies the predicted amount of risk, and a distance metric which quantifies the uncertainty of the risk.
Nominal Stiffness of GT-2 Rubber-Fiberglass Timing Belts for Dynamic System Modeling and Design
Authors: Wang Bozun , Si Yefei , Chadha Charul , Allison James T. , Patterson Albert E.
Published: November 2018

GT-style rubber-fiberglass (RF) timing belts are designed to effectively transfer rotational motion from pulleys to linear motion in robots, small machines, and other important mechatronic systems. One of the characteristics of belts under this type of loading condition is that the length between load and pulleys changes during operation, thereby changing their effective stiffness. It has been shown that the effective stiffness of such a belt is a function of a “nominal stiffness” and the real-time belt section lengths. However, this nominal stiffness is not necessarily constant; it is common to assume linear proportional stiffness, but this often results in system modeling error. This technical note describes a brief study where the nominal stiffness of two lengths ( 400 m m and 760 m m ) of GT-2 RF timing belt was tested up to breaking point; regression analysis was performed on the results to best model the observed stiffness. The experiments were performed three times, providing a total of six stiffness curves. It was found that cubic regression mod els ( R 2 > 0.999 ) were the best fit, but that quadratic and linear models still provided acceptable representations of the whole dataset with R 2 values above 0.940 .
Landscape-Scale Aboveground Biomass Estimation in Buffer Zone Community Forests of Central Nepal: Coupling In Situ Measurements with Landsat 8 Satellite Data
Authors: Pandit Santa , Tsuyuki Satoshi , Dube Timothy
Published: November 2018

Knowledge of forest productivity status is an important indicator of the amount of biomass accumulated and the role of terrestrial ecosystems in the carbon cycle. However, accurate and up-to-date information on forest biomass and forest succession remain rudimentary within natural forests. This study sought to understand and establish the potential of a new-generation sensor in estimating aboveground biomass (AGB) stored in the natural forest, also known as ‘community forest’ or buffer zone community forest (BZCF), in the Parsa National Park, Nepal. The utility of the 30-m resolution Landsat 8 Operational Land Imager (OLI) and in situ data was tested using two statistical approaches, namely multiple linear regression (MLR) and random forest (RF). The analysis was done based on four computational procedures. These included spectral bands, vegetation indices and pooled dataset (spectral bands + vegetation indices), and model selected important variables. AGB estimation based on pooled data showed that the RF algorithm produced better results when compared to the use of the MLR model. For instance, the RF model estimated AGB with an R2 value of 0.87 and a root mean square error of 20.50 t ha−1, as well as an R2 value of 0.95 and a RMSE of 13.3 t ha−1 when using selected important variables. Comparatively, the MLR using pooled data produced an R2 value of 0.56 and RMSE value of 37.01 t ha−1. The RF model selected Optimized Soil Adjusted Vegetation index (OSAVI), Simple ratio (SR), Modified simple ratio (MSR), and Normalized difference Vegetation index (NDVI) as the most important variables for estimating AGB, whereas MLR selected band 5 and SR. These findings demonstrate the relevance of the relatively new Landsat 8 sensor in the estimation of AGB in community buffer zones.
Correction: Hu, A. Using Bidirectional Long Short-Term Memory Method for the Height of F2 Peak Forecasting from Ionosonde Measurements in the Australian Region. Remote Sens. 2018, 10, 1658
Authors: Hu Andong , Zhang Kefei
Published: November 2018

After publication of the research paper [...]