bluuebunny/crossref_metadata_2025 huggingface dataset:
Fatal Pediatric Motor Vehicle Crashes on U.S. Native American Indian Lands Compared to Adjacent Non-Indian Lands: Restraint Use and Injury by Driver, Vehicle, Roadway and Crash Characteristics
Authors: Oh Shin , Liu Chang , Pressley Joyce
Published: October 2017

There are large disparities in American Indian pediatric motor vehicle (MV) mortality with reports that several factors may contribute. The Fatality Analysis Reporting System for 2000–2014 was used to examine restraint use for occupants aged 0–19 years involved in fatal MV crashes on Indian lands (n = 1667) and non-Indian lands in adjacent states (n = 126,080). SAS GLIMMIX logistic regression with random effects was used to generate odds ratios (OR) with 95% confidence intervals (CI). Restraint use increased in both areas over the study period with restraint use on Indian lands being just over half that of non-Indian lands for drivers (36.8% vs. 67.8%, p < 0.0001) and for pediatric passengers (33.1% vs. 59.3%, p < 0.0001). Driver restraint was the strongest predictor of passenger restraint on both Indian and non-Indian lands exerting a stronger effect in ages 13–19 than in 0–12 year olds. Valid licensed driver was a significant predictor of restraint use in ages 0–12 years. Passengers in non-cars (SUVs, vans and pickup trucks) were less likely to be restrained. Restraint use improved over the study period in both areas, but disparities failed to narrow as restraint use remains lower and driver, vehicle and crash risk factors higher for MV mortality on Indian lands.
FGF-2b and h-PL Transform Duct and Non-Endocrine Human Pancreatic Cells into Endocrine Insulin Secreting Cells by Modulating Differentiating Genes
Authors: Donadel Giulia , Pastore Donatella , Della-Morte David , Capuani Barbara , Lombardo Marco , Pacifici Francesca , Bugliani Marco , Grieco Fabio , Marchetti Piero , Lauro Davide
Published: October 2017

Background: Diabetes mellitus (DM) is a multifactorial disease orphan of a cure. Regenerative medicine has been proposed as novel strategy for DM therapy. Human fibroblast growth factor (FGF)-2b controls β-cell clusters via autocrine action, and human placental lactogen (hPL)-A increases functional β-cells. We hypothesized whether FGF-2b/hPL-A treatment induces β-cell differentiation from ductal/non-endocrine precursor(s) by modulating specific genes expression. Methods: Human pancreatic ductal-cells (PANC-1) and non-endocrine pancreatic cells were treated with FGF-2b plus hPL-A at 500 ng/mL. Cytofluorimetry and Immunofluorescence have been performed to detect expression of endocrine, ductal and acinar markers. Bromodeoxyuridine incorporation and annexin-V quantified cells proliferation and apoptosis. Insulin secretion was assessed by RIA kit, and electron microscopy analyzed islet-like clusters. Results: Increase in PANC-1 duct cells de-differentiation into islet-like aggregates was observed after FGF-2b/hPL-A treatment showing ultrastructure typical of islets-aggregates. These clusters, after stimulation with FGF-2b/hPL-A, had significant (p < 0.05) increase in insulin, C-peptide, pancreatic and duodenal homeobox 1 (PDX-1), Nkx2.2, Nkx6.1, somatostatin, glucagon, and glucose transporter 2 (Glut-2), compared with control cells. Markers of PANC-1 (Cytokeratin-19, MUC-1, CA19-9) were decreased (p < 0.05). These aggregates after treatment with FGF-2b/hPL-A significantly reduced levels of apoptosis. Conclusions: FGF-2b and hPL-A are promising candidates for regenerative therapy in DM by inducing de-differentiation of stem cells modulating pivotal endocrine genes.
A Rapid One-Step Process for Fabrication of Biomimetic Superhydrophobic Surfaces by Pulse Electrodeposition
Authors: Jiang Shuzhen , Guo Zhongning , Liu Guixian , Gyimah Glenn , Li Xiaoying , Dong Hanshan
Published: October 2017

Inspired by some typical plants such as lotus leaves, superhydrophobic surfaces are commonly prepared by a combination of low surface energy materials and hierarchical micro/nano structures. In this work, superhydrophobic surfaces on copper substrates were prepared by a rapid, facile one-step pulse electrodepositing process, with different duty ratios in an electrolyte containing lanthanum chloride (LaCl3·6H2O), myristic acid (CH3(CH2)12COOH), and ethanol. The equivalent electrolytic time was only 10 min. The surface morphology, chemical composition and superhydrophobic property of the pulse electrodeposited surfaces were fully investigated with SEM, EDX, XRD, contact angle meter and time-lapse photographs of water droplets bouncing method. The results show that the as-prepared surfaces have micro/nano dual scale structures mainly consisting of La[CH3(CH2)12COO]3 crystals. The maximum water contact angle (WCA) is about 160.9°, and the corresponding sliding angle is about 5°. This method is time-saving and can be easily extended to other conductive materials, having a great potential for future applications.
Determination of the Deacetylation Degree of Chitooligosaccharides
Authors: Jiang Yao , Fu Chuhan , Wu Sihui , Liu Guihua , Guo Jiao , Su Zhengquan
Published: October 2017

The methods for determination of chitosan content recommended in the Chinese Pharmacopoeia and the European Pharmacopoeia are not applicable for evaluation of the extent of deacetylation (deacetylation degree, DD) in chitooligosaccharides (COS). This study explores two different methods for assessment of DD in COS having relatively high and low molecular weights: an acid-base titration with bromocresol green indicator and a first order derivative UV spectrophotometric method for assessment of DD in COS. The accuracy of both methods as a function of molecular weight was also investigated and compared to results obtained using 1H NMR spectroscopy. Our study demonstrates two simple, fast, widely adaptable, highly precise, accurate, and inexpensive methods for the effective determination of DD in COS, which have the potential for widespread commercial applications in developing country.
Association, Distribution, Liberation, and Rheological Balances of Alkyldimethylbenzylammonium Chlorides (C12–C16)
Authors: Vitková Zuzana , Oremusová Jarmila , Herdová Petra , Ivánková Oľga , Vitko Anton
Published: October 2017

It is known that cationic surfactants have an antimicrobial effect and act as enhancers. This paper studies three cationic surfactants from the group of alkyldimethylbenzylammonium chlorides (dodecyl-, tetradecyl-, and hexadecyl). Interest is focused on the association of the surfactants with respect to temperature, partition balances and their influence on drug release, rheological properties, and the pH of hydrogels. The critical micelle concentrations (CMC) of the surfactants were estimated from dependencies of conductivity, density, spectrofluorimetry, and UV–VIS spectrophotometry on molarity in the temperature range of 25–50 °C. It was found that the temperature dependence of a CMC is U-shaped, with its minimum at 30 °C, and the CMC value decreases as the length of the chain increases. The pseudo-phase separation model was used for the calculation of various thermodynamic parameters, such as the Gibbs free energies (spontaneous process), enthalpies (exothermic process), and entropies of the micelles’ formation, CMCs, and the degree of counterion binding. All thermodynamic parameters, as functions of the temperature, were estimated. It was found that partition coefficients increase as the length of the alkyl chain and the pH = (5.0–7.0) increase. The influences of surfactants, below and above the CMC, on drug (chlorhexidine dihydrochloride) release from hydrogels, rheological properties, and pH at 30 °C were studied. Also, the amounts of the released drug increase as the alkyl chains of the surfactants prolongate. The amounts of the released drug with the surfactant below the CMC are greater than that above the CMC. All hydrogels (regardless of the length of the alkyl chain) exhibit a non-Newtonian pseudo-plastic flow. The results obtained will be used in the formulation of the drug and surfactants into dosage forms.
Unraveling the Origin of Magnetism in Mesoporous Cu-Doped SnO2 Magnetic Semiconductors
Authors: Fan Junpeng , Menéndez Enric , Guerrero Miguel , Quintana Alberto , Weschke Eugen , Pellicer Eva , Sort Jordi
Published: October 2017

The origin of magnetism in wide-gap semiconductors doped with non-ferromagnetic 3d transition metals still remains intriguing. In this article, insights in the magnetic properties of ordered mesoporous Cu-doped SnO2 powders, prepared by hard-templating, have been unraveled. Whereas, both oxygen vacancies and Fe-based impurity phases could be a plausible explanation for the observed room temperature ferromagnetism, the low temperature magnetism is mainly and unambiguously arising from the nanoscale nature of the formed antiferromagnetic CuO, which results in a net magnetization that is reminiscent of ferromagnetic behavior. This is ascribed to uncompensated spins and shape-mediated spin canting effects. The reduced blocking temperature, which resides between 30 and 5 K, and traces of vertical shifts in the hysteresis loops confirm size effects in CuO. The mesoporous nature of the system with a large surface-to-volume ratio likely promotes the occurrence of uncompensated spins, spin canting, and spin frustration, offering new prospects in the use of magnetic semiconductors for energy-efficient spintronics.
In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes
Authors: Barta Csengele , Bolander Bethany , Bilby Steven , Brown Jeremy , Brown Reid , Duryee Alexander , Edelman Danielle , Gray Christina , Gossett Chandler , Haddock Amie , Helsel Mackenzie , Jones Alyssa , Klingseis Marissa , Leslie Kalif , Miles Edward , Prawitz Rachael
Published: October 2017

Mature oak (Quercus spp.) leaves, although abundantly available during the plants’ developmental cycle, are rarely exploited as viable sources of genomic DNA. These leaves are rich in metabolites difficult to remove during standard DNA purification, interfering with downstream molecular genetics applications. The current work assessed whether in situ dark adaptation, to deplete sugar reserves and inhibit secondary metabolite synthesis could compensate for the difficulties encountered when isolating DNA from mature leaves rich in secondary metabolites. We optimized a rapid, commercial kit based method to extract genomic DNA from dark- and light-adapted leaves. We demonstrated that in situ dark adaptation increases the yield and quality of genomic DNA obtained from mature oak leaves, yielding templates of sufficiently high quality for direct downstream applications, such as PCR amplification and gene identification. The quality of templates isolated from dark-adapted pin oak leaves particularly improved the amplification of larger fragments in our experiments. From DNA extracts prepared with our optimized method, we identified for the first time partial segments of the genes encoding 18S rRNA and isoprene synthase (IspS) from pin oak (Quercus palustris), whose full genome has not yet been sequenced.
Formose Reaction Controlled by a Copolymer of N,N-Dimethylacrylamide and 4-Vinylphenylboronic Acid
Authors: Michitaka Tomohiro , Imai Toru , Hashidzume Akihito
Published: October 2017

The formose reaction is an oligomerization of formaldehyde under basic conditions, which produces a complicated mixture of monosaccharides and sugar alcohols. Selective formation of useful monosaccharides by the formose reaction has been an important challenge. In this study, we have investigated the formose reaction controlled by N,N-dimethylacrylamide/4-vinylphenylboronic acid copolymer (pDMA/VBA) and phenylboronic acid (PBA) because boronic acid compounds form esters with polyols, e.g., monosaccharides and sugar alcohols. We obtained time–conversion data in the presence of these boronic acid compounds, and characterized the products by liquid chromatography-mass spectroscopy and NMR measurements. pDMA/VBA and PBA decelerated the formose reaction because of the formation of boronic acid esters with products. It is noteworthy that the formose reaction in the presence of pDMA/VBA and PBA formed favorably six- and seven-carbon branched monosaccharides and sugar alcohols.
Preparation, Characterization and Application of UV-Curable Flexible Hyperbranched Polyurethane Acrylate
Authors: Xiang Hongping , Wang Xiaowei , Lin Guanghong , Xi Lu , Yang Yan , Lei Dehua , Dong Haihui , Su Jiahui , Cui Yanyan , Liu Xiaoxuan
Published: October 2017

A novel UV-curable hyperbranched polyurethane acrylate (FHBPUA) with excellent flexibility is successfully synthesized based on a reaction of hydroxyl terminated hyperbranched polyurethane (regarded as core) with flexible semiadduct urethane monoacrylate (regarded as arms). The structure and property of FHBPUA is firstly analyzed and then utilized as functional additives to ameliorate the UV-curing and mechanical properties of epoxy acrylate resin. The degree of branching of FHBPUA turns out to be 0.82. Its thermal decomposition process consists of three different stages, and the glass transition temperature is around 65 °C. The freestanding FHBPUA film (~30 μm thickness) can be UV-cured within 3 s, and its flexibility is up to 1 mm. With the increase of FHBPUA content to 10 wt %, the UV-curing time of UV1000 film decreases from 6 to 3 s, flexibility strikingly increases from 10 to 1 mm, and adhesive force also improves from 5 to 3 grades, meanwhile its glossiness is not influenced by FHBPUA. In addition, a certain amount of FHBPUA can improve the tensile strength and elongation at break of UV1000 film. This novel FHBPUA can be used not only to develop flexible UV-curable freestanding films but also as functional additives to perfect other UV-curable compositions like coatings, inks and 3D printed parts.
Arrhythmia Evaluation in Wearable ECG Devices
Authors: Sadrawi Muammar , Lin Chien-Hung , Lin Yin-Tsong , Hsieh Yita , Kuo Chia-Chun , Chien Jen , Haraikawa Koichi , Abbod Maysam , Shieh Jiann-Shing
Published: October 2017

This study evaluates four databases from PhysioNet: The American Heart Association database (AHADB), Creighton University Ventricular Tachyarrhythmia database (CUDB), MIT-BIH Arrhythmia database (MITDB), and MIT-BIH Noise Stress Test database (NSTDB). The ANSI/AAMI EC57:2012 is used for the evaluation of the algorithms for the supraventricular ectopic beat (SVEB), ventricular ectopic beat (VEB), atrial fibrillation (AF), and ventricular fibrillation (VF) via the evaluation of the sensitivity, positive predictivity and false positive rate. Sample entropy, fast Fourier transform (FFT), and multilayer perceptron neural network with backpropagation training algorithm are selected for the integrated detection algorithms. For this study, the result for SVEB has some improvements compared to a previous study that also utilized ANSI/AAMI EC57. In further, VEB sensitivity and positive predictivity gross evaluations have greater than 80%, except for the positive predictivity of the NSTDB database. For AF gross evaluation of MITDB database, the results show very good classification, excluding the episode sensitivity. In advanced, for VF gross evaluation, the episode sensitivity and positive predictivity for the AHADB, MITDB, and CUDB, have greater than 80%, except for MITDB episode positive predictivity, which is 75%. The achieved results show that the proposed integrated SVEB, VEB, AF, and VF detection algorithm has an accurate classification according to ANSI/AAMI EC57:2012. In conclusion, the proposed integrated detection algorithm can achieve good accuracy in comparison with other previous studies. Furthermore, more advanced algorithms and hardware devices should be performed in future for arrhythmia detection and evaluation.
Dolphin Sounds-Inspired Covert Underwater Acoustic Communication and Micro-Modem
Authors: Qiao Gang , Zhao Yunjiang , Liu Songzuo , Bilal Muhammad
Published: October 2017

A novel portable underwater acoustic modem is proposed in this paper for covert communication between divers or underwater unmanned vehicles (UUVs) and divers at a short distance. For the first time, real dolphin calls are used in the modem to realize biologically inspired Covert Underwater Acoustic Communication (CUAC). A variety of dolphin whistles and clicks stored in an SD card inside the modem helps to realize different biomimetic CUAC algorithms based on the specified covert scenario. In this paper, the information is conveyed during the time interval between dolphin clicks. TMS320C6748 and TLV320AIC3106 are the core processors used in our unique modem for fast digital processing and interconnection with other terminals or sensors. Simulation results show that the bit error rate (BER) of the CUAC algorithm is less than 10 − 5 when the signal to noise ratio is over ‒5 dB. The modem was tested in an underwater pool, and a data rate of 27.1 bits per second at a distance of 10 m was achieved.
NFC Evaluation in the Development of Mobile Applications for MICE in Tourism
Authors: Silva-Pedroza David , Marin-Calero Ricardo , Ramirez-Gonzalez Gustavo
Published: October 2017

This paper presents an analysis and implementation of a service for the deployment of events in the Meetings, Incentives, Conferences, and Exhibitions (MICE) category, to answer the question: how can Near Field Communication (NFC) and mobile applications contribute to the development of tourism in the MICE category? First is an analysis of the applications that are currently on the market and an extraction of the features of greater relevance; later, we define the functionalities for our service, and finally we provide a performance test in a MICE-type event, the seventh Seminar on Emerging Technologies in Telecommunications “TET 2016” developed in Popayán, Colombia and the results of the experience are analyzed. The use of NFC technology with a mobile application allows the experience to be improved when a MICE event was made, for both the user and the organizer.
Design, Prototyping, and Assessment of a Wastewater Closed-Loop Recovery and Purification System
Authors: Bortolini Marco , Gamberi Mauro , Mora Cristina , Pilati Francesco , Regattieri Alberto
Published: October 2017

Efforts to decrease the water use within industry are mandatory to pursue product and process sustainability. Particularly, the European Union (EU) is at the top level for water consumption in industry, while some sectors, such as the food and beverage (F&B), are highly water-intensive with hundreds of liters per hour of consumed and, then, drained water. This article provides a systematic overview of the most innovative insights coming from an EU Eco-Innovation project dealing with greening the F&B industry through the design, prototyping, technical, economic, and environmental assessment of a wastewater closed-loop recovery and purification system. The system, tailored for a standard mid-size F&B company using 2–3 billion L/year of raw water, collects, purifies and recirculates the key produced wastewater streams with an overall recovery efficiency of about 56%. The proposed purification technology comes from the most efficient combination of membrane-based filtration methods, reverse osmosis (RO), and ultraviolet modules. Evidence from the technical design, full-scale on-site technology prototyping, net-present-value (NPV) analysis and system life-cycle-assessment (LCA) are presented concluding about the convenience of adopting the proposed solution to reduce costs and impacts on the environment.
Shiga Toxin Glycosphingolipid Receptors in Human Caco-2 and HCT-8 Colon Epithelial Cell Lines
Authors: Kouzel Ivan , Pohlentz Gottfried , Schmitz Julia , Steil Daniel , Humpf Hans-Ulrich , Karch Helge , Müthing Johannes
Published: October 2017

Shiga toxins (Stxs) released by enterohemorrhagic Escherichia coli (EHEC) into the human colon are the causative agents for fatal outcome of EHEC infections. Colon epithelial Caco-2 and HCT-8 cells are widely used for investigating Stx-mediated intestinal cytotoxicity. Only limited data are available regarding precise structures of their Stx receptor glycosphingolipids (GSLs) globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer), and lipid raft association. In this study we identified Gb3Cer and Gb4Cer lipoforms of serum-free cultivated Caco-2 and HCT-8 cells, chiefly harboring ceramide moieties composed of sphingosine (d18:1) and C16:0, C22:0 or C24:0/C24:1 fatty acid. The most significant difference between the two cell lines was the prevalence of Gb3Cer with C16 fatty acid in HCT-8 and Gb4Cer with C22–C24 fatty acids in Caco-2 cells. Lipid compositional analysis of detergent-resistant membranes (DRMs), which were used as lipid raft-equivalents, indicated slightly higher relative content of Stx receptor Gb3Cer in DRMs of HCT-8 cells when compared to Caco-2 cells. Cytotoxicity assays revealed substantial sensitivity towards Stx2a for both cell lines, evidencing little higher susceptibility of Caco-2 cells versus HCT-8 cells. Collectively, Caco-2 and HCT-8 cells express a plethora of different receptor lipoforms and are susceptible towards Stx2a exhibiting somewhat lower sensitivity when compared to Vero cells.
Investigation of Non-Covalent Interactions of Aflatoxins (B1, B2, G1, G2, and M1) with Serum Albumin
Authors: Poór Miklós , Bálint Mónika , Hetényi Csaba , Gődér Beatrix , Kunsági-Máté Sándor , Kőszegi Tamás , Lemli Beáta
Published: October 2017

Aflatoxins are widely spread mycotoxins produced mainly by Aspergillus species. Consumption of aflatoxin-contaminated foods and drinks causes serious health risks for people worldwide. It is well-known that the reactive epoxide metabolite of aflatoxin B1 (AFB1) forms covalent adducts with serum albumin. However, non-covalent interactions of aflatoxins with human serum albumin (HSA) are poorly characterized. Thus, in this study the complex formation of aflatoxins was examined with HSA applying spectroscopic and molecular modelling studies. Our results demonstrate that aflatoxins form stable complexes with HSA as reflected by binding constants between 2.1 × 104 and 4.5 × 104 dm3/mol. A binding free energy value of −26.90 kJ mol−1 suggests a spontaneous binding process between AFB1 and HSA at room-temperature, while the positive entropy change of 55.1 JK−1 mol−1 indicates a partial decomposition of the solvation shells of the interacting molecules. Modeling studies and investigations with site markers suggest that Sudlow’s Site I of subdomain IIA is the high affinity binding site of aflatoxins on HSA. Interaction of AFB1 with bovine, porcine, and rat serum albumins was also investigated. Similar stabilities of the examined AFB1-albumin complexes were observed suggesting the low species differences of the albumin-binding of aflatoxins.
Oral Delivery of Probiotics Expressing Dendritic Cell-Targeting Peptide Fused with Porcine Epidemic Diarrhea Virus COE Antigen: A Promising Vaccine Strategy against PEDV
Authors: Wang Xiaona , Wang Li , Huang Xuewei , Ma Sunting , Yu Meiling , Shi Wen , Qiao Xinyuan , Tang Lijie , Xu Yigang , Li Yijing
Published: October 2017

Porcine epidemic diarrhea virus (PEDV), an enteric coronavirus, is the causative agent of porcine epidemic diarrhea (PED) that damages intestinal epithelial cells and results in severe diarrhea and dehydration in neonatal suckling pigs with up to 100% mortality. The oral vaccine route is reported as a promising approach for inducing protective immunity against PEDV invasion. Furthermore, dendritic cells (DCs), professional antigen-presenting cells, link humoral and cellular immune responses for homeostasis of the intestinal immune environment. In this study, in order to explore an efficient oral vaccine against PEDV infection, a mucosal DC-targeting oral vaccine was developed using Lactobacillus casei to deliver the DC-targeting peptide (DCpep) fused with the PEDV core neutralizing epitope (COE) antigen. This probiotic vaccine could efficiently elicit secretory immunoglobulin A (SIgA)-based mucosal and immunoglobulin G (IgG)-based humoral immune responses via oral vaccination in vivo. Significant differences (p < 0.05) in the immune response levels were observed between probiotics expressing the COE-DCpep fusion protein and COE antigen alone, suggesting better immune efficiency of the probiotics vaccine expressing the DC-targeting peptide fused with PEDV COE antigen. This mucosal DC-targeting oral vaccine delivery effectively enhances vaccine antigen delivery efficiency, providing a useful strategy to induce efficient immune responses against PEDV infection.
Substrate Composition and Depth Affect Soil Moisture Behavior and Plant-Soil Relationship on Mediterranean Extensive Green Roofs
Authors: Chenot Julie , Gaget Elie , Moinardeau Cannelle , Jaunatre Renaud , Buisson Elise , Dutoit Thierry
Published: October 2017

The Mediterranean basin is extremely vulnerable to climate change, and one of the areas most impacted by human water demand. Yet the green roofs increasingly created both for aesthetic reasons and to limit pollution and urban runoff are themselves very water-demanding. Successful green roof installation depends on the establishment of the vegetation, and the substrate is the key element: it conserves water, and provides the nutrients and physical support indispensable for plant growth. Since typical Mediterranean plant communities require no maintenance, this study seeks to develop techniques for creating maintenance- and watering-free horizontal green roofs for public or private buildings in a Mediterranean context. The innovative aspect of this study lies in creating two soil mixes, fine elements (clay and silt) and coarse elements (pebbles of all sizes), in two different thicknesses, to assess vegetation development. Monitoring of substrate moisture was carried out and coupled with local rainfall measurements during summer and autumn. As expected, substrate moisture is mainly influenced by substrate depth (the deeper, the moister) and composition (the finer the particles (clays and silts), the higher the moisture content). Vegetation cover impacts moisture to a lesser extent but is itself affected by the composition and depth of the substrates. These results are subsequently discussed with relation to the issue of sustainable green roofs in Mediterranean climates. Considering applications of our results, for an optimal colonization of a Mediterranean vegetation, a substrate thickness of 15 cm composed mainly of fine elements (75% clay-silt and 25% pebble-sand) would be recommended in green roofs.
Spatial and Temporal Dynamics of Potentially Toxic Cyanobacteria in the Riverine Region of a Temperate Estuarine System Altered by Weirs
Authors: Malazarte Jacqueline , Lee Hakyoung , Kim Hyun-Woo , Sin Yongsik
Published: October 2017

The effects of weirs on fish and other biological communities have garnered considerable study, whereas the effects of weirs on community composition of toxic cyanobacteria have not yet been well documented. In this study, temporal and spatial variations in species composition and the abundance of potentially toxic cyanobacteria were investigated in the riverine regions of the temperate Youngsan River estuary, where two weirs have recently been constructed. Four stations were sampled 0.5 m below the surface monthly along the channel of the upper river from May 2014 to April 2015 to explore cyanobacterial composition and abundance, while physicochemical and biological parameters were measured to elucidate possible mechanisms controlling these dynamics. Two stations were located upstream at free-flowing sites, and the other stations were located downstream at impounded sites near the weirs. Twenty-eight cyanobacterial species were identified, seven of which were potentially toxic: Microcystis sp., M. aeruginosa, M. flos-aquae, Dolichospermum sp., Aphanocapsa sp., Oscillatoria sp. and Phormidium sp. Microcystis sp. was the most abundant in June 2014 at the lowest station near the weir. Meanwhile, Phormidium sp. occurred at low abundance throughout the study period, except during the winter months, when its abundance was elevated. The interactive forward selection method highlighted dissolved inorganic nitrogen and zooplankton abundance as explanatory variables for this observed variation, but their effects on cyanobacterial growth are unclear. However, temperature was the major determinant for the temporal variation in cyanobacterial populations. Cluster analysis showed that the downstream stations near the weirs had a high similarity of potentially toxic cyanobacteria. Significantly higher abundance, especially of Microcystis sp., was also recorded at the impounded sites suggesting that the presence of weirs might affect variations in toxic cyanobacterial communities.
Examining the Behavioral Intentions of Bungee Jumping Tourists at the Macau Tower: A Quantitative Analysis Using Structural Equation Modeling
Authors: Wu Hung-Che , Cheng Ching-Chan , Chen Yi-Chang
Published: October 2017

This study aims to examine the structural relationships among experiential quality, perceived authenticity, novelty seeking, experiential satisfaction, and behavioral intentions using a multidimensional and hierarchical model. The findings of this study are based on structural equation
modeling (SEM) analysis of a convenience sampling of 410 tourists completing experiencing bungee jumping at the Macau Tower. The results indicate that the model proposed by this study fits the data. The analysis results contribute to the services marketing theory by providing additional insights
into experiential quality, perceived authenticity, novelty seeking, experiential satisfaction, behavioral intentions, and the dimensions of experiential quality.
Au55, a stable glassy cluster: results of ab initio calculations
Authors: Vollath Dieter , Holec David , Fischer Franz Dieter
Published: October 2017

Structure and properties of small nanoparticles are still under discussion. Moreover, some thermodynamic properties and the structural behavior still remain partially unknown. One of the best investigated nanoparticles is the Au55 cluster, which has been analyzed experimentally and theoretically. However, up to now, the results of these studies are still inconsistent. Consequently, we have carried out the present ab initio study of the Au55 cluster, using up-to-date computational concepts, in order to clarify these issues. Our calculations have confirmed the experimental result that the thermodynamically most stable structure is not crystalline, but it is glassy. The non-crystalline structure of this cluster was validated by comparison of the coordination numbers with those of a crystalline cluster. It was found that, in contrast to bulk materials, glass formation is connected to an energy release that is close to the melting enthalpy of bulk gold. Additionally, the surface energy of this cluster was calculated using two different theoretical approaches resulting in values close to the surface energy for bulk gold. It shall be emphasized that it is now possible to give a confidence interval for the value of the surface energy.
Broad Susceptibility of Nucleolar Proteins and Autoantigens to Complement C1 Protease Degradation
Authors: Cai Yitian , Wee Seng Yin Kelly , Chen Junjie , Teo Boon Heng Dennis , Ng Yee Leng Carol , Leong Khai Pang , Lu Jinhua
Published: October 2017

Anti-nuclear autoantibodies, which frequently target the nucleoli, are pathogenic hallmarks of systemic lupus erythematosus (SLE). Although the causes of these Abs remain broad and ill-defined, a genetic deficiency in C1 complex (C1qC1r2C1s2) or C4 is able to induce these Abs. Considering a recent finding that, in dead cells, nucleoli were targeted by C1q and two nucleolar autoantigens were degraded by C1r/C1s proteases, we considered that C1 could help protect against antinuclear autoimmunity by broadly degrading nucleolar proteins or autoantigens. Nucleoli were isolated to homogeneity and structurally defined. After C1 treatment, cleaved nucleolar proteins were identified by proteomic two-dimensional fluorescence difference gel electrophoresis and mass spectrometry, and further verified by Western blotting using specific Abs. The extent of nucleolar autoantigen degradation upon C1 treatment was estimated using SLE patient autoantibodies. The isolated nucleoli were broadly reactive with SLE patient autoantibodies. These nucleoli lacked significant autoproteolysis, but many nucleolar proteins and autoantigens were degraded by C1 proteases; >20 nucleolar proteins were identified as C1 cleavable. These were further validated by Western blotting using specific Abs. The broad autoantigenicity of the nucleoli may attribute to their poor autoproteolysis, causing autologous immune stimulation upon necrotic exposure. However, C1q targets at these nucleoli to cause C1 protease activation and the cleavage of many nucleolar proteins or autoantigens. This may represent one important surveillance mechanism against antinuclear autoimmunity because C1 genetic deficiency causes anti-nuclear autoantibodies and SLE disease.
Complement Regulatory Protein Factor H Is a Soluble Prion Receptor That Potentiates Peripheral Prion Pathogenesis
Authors: Kane Sarah J. , Farley Taylor K. , Gordon Elizabeth O. , Estep Joshua , Bender Heather R. , Moreno Julie A. , Bartz Jason , Telling Glenn C. , Pickering Matthew C. , Zabel Mark D.
Published: October 2017

Several complement proteins exacerbate prion disease, including C3, C1q, and CD21/35. These proteins of the complement cascade likely increase uptake, trafficking, and retention of prions in the lymphoreticular system, hallmark sites of early prion propagation. Complement regulatory protein factor H (fH) binds modified host proteins and lipids to prevent C3b deposition and, thus, autoimmune cell lysis. Previous reports show that fH binds various conformations of the cellular prion protein, leading us to question the role of fH in prion disease. In this article, we report that transgenic mice lacking Cfh alleles exhibit delayed peripheral prion accumulation, replication, and pathogenesis and onset of terminal disease in a gene-dose manner. We also report a biophysical interaction between purified fH and prion rods enriched from prion-diseased brain. fH also influences prion deposition in brains of infected mice. We conclude from these data and previous findings that the interplay between complement and prions likely involves a complex balance of prion sequestration and destruction via local tissue macrophages, prion trafficking by B and dendritic cells within the lymphoreticular system, intranodal prion replication by B and follicular dendritic cells, and potential prion strain selection by CD21/35 and fH. These findings reveal a novel role for complement-regulatory proteins in prion disease.
A Model for Implementing Integrative Practice in Health Care Agencies
Authors: Patterson Chris , Arthur Heather M.
Published: October 2017

Over the last few years, there has been increased awareness and use of complementary/alternative therapies (CAM) in many countries without the health care infrastructure to support it. The National Centre for Complementary and Alternative Medicine referred to the combining of mainstream medical therapies and CAM as integrative medicine. The creation of integrative health care teams will definitely result in redefining roles, but more importantly in a change in how services are delivered. The purpose of this paper is to describe a model of the necessary health care agency resources to support an integrative practice model. A logic model is used to depict the findings of a review of current evidence. Logic models are designed to show relationships between the goals of a program or initiative, the resources to achieve desired outputs and the activities that lead to outcomes. The four major resource categories necessary for implementing integrative care are within the domains of a) professional and research development, b) health human resource planning, c) regulation and legislation and d) practice and management in clinical areas. It was concluded that the system outcomes from activities within these resource categories should lead to freedom of choice in health care; a culturally sensitive health care system and a broader spectrum of services for achieving public health goals.
Do Mound Disturbance and Bait Placement Affect Bait Removal and Treatment Efficacy in Red Imported Fire ant (Hymenoptera: Formicidae) at Different Seasons?
Authors: Hu Xing P. , Ding Wei
Published: October 2017

This study provides empirical evidence that disturbing mound immediately before application, as opposed to label recommendation, did not reduce foraging activity of the red imported fire ant, Solenopsis invicta Buren, except for about 10-min delay in foraging. Despite the delayed foraging, there was no significant difference in the amount of baits foraged between disturbed and undisturbed colonies. Eventually, >96% of the baits were foraged, with the maximum removal occurred by 2 and 3 h, respectively, in summer and spring trial. The fastest and great amount of bait removal 1 h post-treatment occurred to baits placed on mound, followed by 0.18–0.3-m from mound base, and the slowest 1.08–1.2-m from mound base. All treatment gave 100% control 1 mo later, regardless of the season, without colony relocation or new colony invasion in the test plots.
A Complementary Alternative Medicine Questionnaire for Young Adults
Authors: Patterson Christine , Arthur Heather
Published: October 2017

Limited information exists on how adolescents decide to use complementary/alternative medicine (CAM). There are also no instruments specific to CAM, for the young adult population, which makes it difficult to explore knowledge in this area. The purpose of this study was to develop and examine the psychometric properties of the CAM Questionnaire for Young Adults which measures young adults’ attitudes about CAM. Participants for this cross-sectional survey were selected from enrolled undergraduate students at an urban university. Factor analysis identified three subscales: 1) positive beliefs about CAM; 2) environmental influence; and 3) psychological comfort. The scale has good internal consistency (Cronbach's alpha = 0.79) and shows beginning demonstration of validity. Its use in this sample revealed that young adults who are female and have used CAM in the past for preventing or treating illness have the most positive attitude towards CAM and the greatest likelihood for continued use. The implication that prevention may play a role in young adults’ attitudes about CAM is a potential focus for future research.
Seeking Mind, Body and Spirit Healing–-Why Some Men with Prostate Cancer Choose CAM (Complementary and Alternative Medicine) over Conventional Cancer Treatments
Authors: White Margaret A. , Verhoef Marja J. , Davison B.J. , Gunn Hal , Cooke Karen
Published: October 2017

Little is known about men with prostate cancer who decline conventional treatment and use only complementary and alternative medicine (CAM).

Objectives

To 1) explore why men decline conventional prostate cancer treatment and use CAM 2) understand the role of holistic healing in their care, and 3) document their recommendations for health care providers.

Methods

Semi-structured interviews and follow-up focus groups.

Sample

Twenty-nine men diagnosed with prostate cancer who declined all recommended conventional treatments and used CAM.

Results

Based on strong beliefs about healing, study participants took control by researching the risks of delaying or declining conventional treatment while using CAM as a first option. Most perceived conventional treatment to have a negative impact on quality of life. Participants sought healing in a broader mind, body, spirit context, developing individualized CAM approaches consistent with their beliefs about the causes of cancer. Most made significant lifestyle changes to improve their health. Spirituality was central to healing for one-third of the sample. Participants recommended a larger role for integrated cancer care.

Conclusion

Men who decline conventional prostate cancer treatment and use CAM only may benefit from a whole person approach to care where physicians support them to play an active role in healing while carefully monitoring their disease status.
Efforts to Achieve Healthy Aging
Authors: Leung Ping-Chung
Published: October 2017

Longevity is a blessing as long as good health is not lost. However, the tendency to have a decline on normal physiological activities is inevitable because of the natural processes of degeneration at all levels: molecular, cellular and organic. Hence, the elderly people frequently suffer from cardiovascular problems and skeletal deteriorations that gradually develop to disabilities. Awareness of factors leading to unhealthy aging has led to the formation of different professional groups that aim at the maintenance of health of aging community. The approach tends to be target orientated for the European and US groups, aiming at hormonal replacements and detoxification. In contrast, the oriental groups have been keeping their traditional belief of prevention and internal balance, using nutritional arrangements and non-strenuous exercise as means of maintaining health.
Escuela Infantil en La Milagrosa, Pamplona, España,
Authors: Arquitectos Pereda Pérez
Published: October 2017

Obra: Escuela Infantil en La Milagrosa, Pamplona. España.

Arquitectos: Carlos Pereda Iglesias y Óscar Pérez Silanes.

Fecha proyecto y obra: 2009/9 y 2011/6
Effects of tropical deep convection on interannual variability of tropical tropopause layer water vapor
Authors: Ye Hao , Dessler Andrew E. , Yu Wandi
Published: October 2017

Abstract. Water vapor interannual variability in the tropical tropopause layer (TTL) is investigated using satellite observations and model simulations. We breakdown the influences of the Brewer-Dobson circulation (BDC), the quasi-biennial oscillation (QBO), and the tropospheric temperature (ΔT) as a function of latitude and longitude using a 2-dimensional multivariable linear regression. This allows us to examine the spatial distribution of the impact on TTL water vapor from these physical processes. In agreement with expectation, we find that the impacts from the BDC and QBO act on TTL water vapor by changing TTL temperature. For ΔT, we find that TTL temperatures alone cannot explain the influence. We hypothesize a moistening role for the evaporation of convective ice from increased deep convection as troposphere warms. Tests with simulations from GEOSCCM and a corresponding trajectory model support this hypothesis.
CH4 emission estimates from an active landfill site inferred from a combined approach of CFD modelling and in situ FTIR measurements
Authors: Sonderfeld Hannah , Bösch Hartmut , Jeanjean Antoine P. R. , Riddick Stuart N. , Allen Grant , Ars Sébastien , Davies Stewart , Harris Neil , Humpage Neil , Leigh Roland , Pitt Joseph
Published: October 2017

Abstract. Globally, the waste sector contributes to nearly a fifth of anthropogenic methane emitted to the atmosphere and is the second largest source of methane in the UK. In recent years great improvements to reduce those emissions have been achieved by the installation of methane recovery systems at landfill sites, and subsequently methane emissions reported in national emission inventories have been reduced. Nevertheless, methane emissions of landfills remain uncertain and quantification of emission fluxes is essential to verify reported emission inventories and to monitor changes in emissions. Here we present a new approach for methane emission quantification from a complex source such as a landfill site by applying a computational fluid dynamics (CFD) model to calibrated in situ measurements of methane as part of a field campaign at a landfill site near Ipswich, UK, in August 2014. The methane distribution for different meteorological scenarios is calculated with the CFD model and compared to methane mole fractions measured by an in situ Fourier-transform infrared (FTIR) spectrometer downwind of the prevailing wind direction. Assuming emissions only from the active site, a mean daytime flux of 0.83 mg m−2 s−1, corresponding to a spatially integrated emission of 53.3 kg h−1, was estimated. The addition of a secondary source area adjacent to the active site, where some methane hotspots were observed, improved the agreement between the simulated and measured methane distribution. As a result, the flux from the active site was reduced slightly to 0.71 mg m−2 s−1 (45.6 kg h−1), and at the same time an additional flux of 0.32 mg m−2 s−1 (30.4 kg h−1) was found from the secondary source area. This highlights the capability of our method to distinguish between different emission areas of the landfill site, which can provide more detailed information about emission source apportionment compared to other methods deriving bulk emissions.
Results of an intercomparison for electric field strength measurements within the German calibration service
Authors: Pape Reiner , Karsten Uwe , Lindner Frank-Michael , Rittmann Frank , von Freeden Joachim , Kleine-Ostmann Thomas , Schrader Thorsten
Published: October 2017

Abstract. In this paper we discuss the results of an intercomparison for electric field strength measurements within the German Calibration Service (Deutscher Kalibrierdienst – DKD). The comparison has been carried out on the field strength value required to reach a display reading of 20 V m−1 of the field probes for frequencies between 100 MHz and 18 GHz. Five laboratories joined the intercomparison including the Physikalisch-Technische Bundesanstalt (PTB), the German National Metrology Institute that keeps the primary standard for electric field strength. As measurement artefacts both a small 1-axis probe usually used as transfer sensor at PTB and a larger 3-axis commercial field probe have been used. While the results agree well for the small field probe and when the larger commercial 3-axis field probe is oriented in the direction of the magnetic field, larger deviations occur, when the larger 3-axis field probe is oriented into the direction of the Poynting vector of the calibration field.
Primary production sensitivity to phytoplankton light attenuation parameter increases with transient forcing
Authors: Kvale Karin F. , Meissner Katrin J.
Published: October 2017

Abstract. Treatment of the underwater light field in ocean biogeochemical models has been attracting increasing interest, with some models moving towards more complex parameterisations. We conduct a simple sensitivity study of a typical, highly simplified parameterisation. In our study, we vary the phytoplankton light attenuation parameter over a range constrained by data during both pre-industrial equilibrated and future climate scenario RCP8.5. In equilibrium, lower light attenuation parameters (weaker self-shading) shift net primary production (NPP) towards the high latitudes, while higher values of light attenuation (stronger shelf-shading) shift NPP towards the low latitudes. Climate forcing magnifies this relationship through changes in the distribution of nutrients both within and between ocean regions. Where and how NPP responds to climate forcing can determine the magnitude and sign of global NPP trends in this high CO2 future scenario. Ocean oxygen is particularly sensitive to parameter choice. Under higher CO2 concentrations, two simulations establish a strong biogeochemical feedback between the Southern Ocean and low-latitude Pacific that highlights the potential for regional teleconnection. Our simulations serve as a reminder that shifts in fundamental properties (e.g. light attenuation by phytoplankton) over deep time have the potential to alter global biogeochemistry.