bluuebunny/crossref_metadata_2025 huggingface dataset:
About between-calibration period
Authors: Redziuk Anatolii , Ageiev Volodymyr , Ustymenko Victor , Volkov O. F. , Lesyk O. S. , Kolobov Kostiantyn , Rychok Sergiy , Volkov R. O. , Kotelnitsky D. O.
Published: June 2020

Analysis of normative-and-legal documents related to calibration of measuring devices and testing equipment was conducted; a methodology with an example of calculating between-calibration period was proposed; some issues of its optimisation were considered.
Keywords: calibration, between-calibration period, methodology, calculation, determination.
Static and Dynamic Characterization and Control of a High-Performance Electro-Hydraulic Actuator
Authors: Sahu Govind N. , Singh Suyash , Singh Aditya , Law Mohit
Published: June 2020

This paper characterizes the static, dynamic, and controlled behavior of a high-performance electro-hydraulic actuator to assess its suitability for use in evaluating machine tool behavior. The actuator consists of a double-acting piston and cylinder arrangement controlled by a servo valve and a separate rear chamber controlled by a separate valve, designed to work in conjunction to generate static forces of up to 7000 N that can be superposed with dynamic forces of up to ±1500 N. This superposition of periodic forces with a non-zero mean makes the actuator capable of applying realistic loading conditions like those experienced by machines during cutting processes. To characterize the performance of this actuator, linearized static and dynamic models are described. Since experiments with the actuator exhibit nonlinear characteristics, the linearized static model is expanded to include the influence of nonlinearities due to flow, leakages, saturations, and due to friction and hysteresis. Since all major nonlinearities are accounted for in the expanded static model, the dynamical model remains linear. Unknown static and dynamical model parameters are calibrated from experiments, and the updated models are observed to capture experimentally observed behavior very well. Validated models are used to tune the proportional and integral gains for the closed-loop control strategy, and the model-based tuning in turn guides appropriate closed-loop control of the actuator to increase its bandwidth to 200 Hz. The statically and dynamically characterized actuator can aid machine tool structural testing. Moreover, the validated models can instruct the design and development of other higher-performance electro-hydraulic actuators, guide the conversion of the actuator into a damper, and also test other advanced control strategies to further improve actuator performance.
Different Ground Vegetation Cover Management Systems to Manage Cynodon dactylon in an Irrigated Vineyard
Authors: Valencia-Gredilla Francisco , Royo-Esnal Aritz , Juárez-Escario Alejandro , Recasens Jordi
Published: June 2020

Ground cover management in vineyards in Spain is focused on minimizing soil erosion and compaction. Such practices have influenced the weed community structure in the inter-rows, contributing to the spread of the high noxious weed Cynodon dactylon (L.) Pers. This fact highlights the need for further investigation of the interaction between ground cover practices and weed control techniques. In this study, the effect of four different ground cover managements (M) in the inter-rows on C. dactylon population dynamics (changes in coverage and frequency) was assessed over three seasons (2015–2017): (M1) a no-till spontaneous vegetation ground cover managed by shredding; (M2) a no-till spontaneous vegetation ground cover managed by shredding plus herbicide application, (M3) tilled soil and spontaneous vegetation growing; and (M4) tilled soil and a barley cover crop seeded (Hordeum vulgare L.). Cynodon dactylon and the other weeds responded differently to the various weed control methods. After three seasons, the barley cover crop was the most efficient management system to control C. dactylon and other weeds. Final soil cover in barley cover crop and tilled soil with spontaneous vegetation were 0.5% and 1.1%, respectively, compared to 3.7% and 7.7% obtained by spontaneous vegetation shredded with and without herbicide application, respectively. In addition, total weed frequency varied from 9.7% for barley cover crop to 45.8% for spontaneous vegetation only shredded. Weed community composition changed due to the pressure exerted by each management and the adaptive strategy of the different species. This study highlights the importance of knowledge of how vegetation management influences weed flora to improve the sustainability of wine grape production systems.
Map-A-Mole: Greenspace Area Influences the Presence and Abundance of the European Mole Talpa europaea in Urban Habitats
Authors: Fellowes Mark D.E. , Acquaah-Harrison Kojo , Angeoletto Fabio , Santos Jeater W.M.C. , da Silva Leandro Deleon , Rocha Elise A. , Pirie Tara J. , Thomas Rebecca L.
Published: June 2020

The European mole Talpa europaea is common across much of Britain. It has a unique fossorial lifestyle, and evidence of its presence is readily identified through the presence of characteristic molehills. Although molehills are often a common sight in urban greenspaces, moles are remarkably understudied, with very few studies to date exploring the urban ecology of moles. Here, we investigate if factors such as greenspace (largely urban parks and playing fields) area, intensity of management, distance to nearest patch, amount of time the patch had been isolated from other green patches, and the amount of urbanization (constructed surfaces) surrounding the patch, influence the distribution and abundance of urban moles. Mole signs (hills and surface runs) were counted in all discrete urban greenspaces (excluding domestic gardens and one private golf course) within an 89.5 km2 area in the UK town of Reading. We found that 17 out of 59 surveyed sites contained moles, with their presence being recorded in greenspaces with a minimum patch area of approximately 0.1 km2 (10 ha). Where present, the abundance of mole territories in the greenspaces was associated with both the area of greenspace and degree of urbanization within 150 m of the patch boundary. While the former was not surprising, the latter outcome may be a consequence of sites with an increased risk of flooding being home to fewer moles, and the surrounding area is also less likely to be built upon. This case study highlights how choices made in designing urban green infrastructure will determine which species survive in urban areas long into the future.
Effects of Fermented Soybean Meal Supplementation on the Growth Performance and Cecal Microbiota Community of Broiler Chickens
Authors: Li Yang , Guo Baozhu , Wu Zhengke , Wang Weiwei , Li Chong , Liu Guohua , Cai Huiyi
Published: June 2020

This study investigated the growth performance, serum immunity, and cecal bacterial microbiota of broilers fed a diet in which soybean meal (SBM) was partially replaced with fermented soybean meal (FSBM) for 36 days. A total of 180 one-day-old male Cobb 500 broilers were randomly divided into three dietary groups (six replicates per group): corn-SBM diet (CC); 25% SBM replaced by FSBM (SC); 50% SBM replaced by FSBM (TC). The average daily gain (ADG) and feed conversion rates (FCR) were higher in SC than CC and TC groups (p < 0.05) during the growth (d 22–36) and whole (d 1–36) phases. No significant difference was observed in ADG and average daily feed intake (ADFI) between CC and TC groups during any phases. Dietary treatments increased serum IgA, IgG, and IgM, Chao 1, observed species, and the abundance of the phylum Fimicutes but decreased the proportion of Proteobacteria (p < 0.05). Dietary treatments increased the abundance of the genera Lachnospiraceae, Lachnoclostridium, Gastranaerophilales, and Lactobacillus but decreased the abundance of Escherichia-Shigella and Clostridiales (p < 0.05). Spearman’s correlations showed that the abundance of Gastranaerophilales was positively correlated with ADG and serum immunity, and the abundance of Lactobacillus was strongly positively with IgM. Thus, replacing 25% of SBM with FSBM improves the growth performance and serum immunity of broilers, possibly due to altered cecal microbial composition.
Anti-Bacterial Effects of Essential Oils against Uropathogenic Bacteria
Authors: Loose Maria , Pilger Emmelie , Wagenlehner Florian
Published: June 2020

Given the increasing antimicrobial resistance in urinary tract infections (UTI), alternative strategies need to be investigated. Determination of minimal inhibitory and bactericidal concentrations of essential oils from cajeput, lemongrass, tea tree, and thyme in artificial urine, revealed bactericidal activity of all four tested essential oils against seven uropathogenic species with values ranging between 0.78–50 mg/mL. Tea tree and thyme essential oils were more efficient than lemongrass and cajeput. In addition, antibiotic-resistant strains showed similar susceptibility as antibiotic-sensitive strains, suggesting no cross-resistance between antibiotics and these essential oils. Checkerboard assays revealed a synergistic activity of the combination of thyme and tea tree. Furthermore, the combination with thyme and tea tree essential oils increased the activity of fosfomycin and pivmecillinam, but not nitrofurantoin, against Escherichia coli. This study provides a basis for further investigation of the potential of thyme and tea tree oil as an alternative or additional treatment of UTI.
Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study
Authors: Alam Prawez , Ezzeldin Essam , Iqbal Muzaffar , Mostafa Gamal A.E. , Anwer Md. Khalid , Alqarni Mohammed H. , Foudah Ahmed I. , Shakeel Faiyaz
Published: June 2020

In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 v/v/v) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at λmax = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.
Development of a Tailored, Complex Intervention for Clinical Reflection and Communication about Suspected Urinary Tract Infections in Nursing Home Residents
Authors: Arnold Sif H. , Olesen Julie A. , Jensen Jette N. , Bjerrum Lars , Holm Anne , Kousgaard Marius B.
Published: June 2020

Background: Inappropriate antibiotic treatments for urinary tract infections (UTIs) in nursing homes cause the development of resistant bacteria. Nonspecific symptoms and asymptomatic bacteriuria are drivers of overtreatment. Nursing home staff provide general practice with information about ailing residents; therefore, their knowledge and communication skills influence prescribing. This paper describes the development of a tailored, complex intervention for a cluster-randomised trial that targets the knowledge of UTI and communication skills in nursing home staff to reduce antibiotic prescriptions. Methods: A dialogue tool was drafted, drawing on participatory observations in nursing homes, interviews with stakeholders, and a survey in general practice. The tool was tailored through a five-phase process that included stakeholders. Finally, the tool and a case-based educational session were tested in a pilot study. Results: The main barriers were that complex patients were evaluated by healthcare staff with limited knowledge about disease and clinical reasoning; findings reported to general practice were insignificant and included vague descriptions; there was evidence of previous opinion bias; nonspecific symptoms were interpreted as UTI; intuitive reasoning led to the inappropriate suspicion of UTI. Conclusion: Sustainable change in antibiotic-prescribing behaviour in nursing homes requires a change in nursing home staff’s beliefs about and management of UTIs.
Plant-Derived Natural Biomolecule Picein Attenuates Menadione Induced Oxidative Stress on Neuroblastoma Cell Mitochondria
Authors: Kesari Kavindra Kumar , Dhasmana Anupam , Shandilya Shruti , Prabhakar Neeraj , Shaukat Ahmed , Dou Jinze , Rosenholm Jessica M. , Vuorinen Tapani , Ruokolainen Janne
Published: June 2020

Several bioactive compounds are in use for the treatment of neurodegenerative disorders, such as Alzheimer’s and Parkinson’s disease. Historically, willow (salix sp.) bark has been an important source of salisylic acid and other natural compounds with anti-inflammatory, antipyretic and analgesic properties. Among these, picein isolated from hot water extract of willow bark, has been found to act as a natural secondary metabolite antioxidant. The aim of this study was to investigate the unrevealed pharmacological action of picein. In silico studies were utilized to direct the investigation towards the neuroprotection abilities of picein. Our in vitro studies demonstrate the neuroprotective properties of picein by blocking the oxidative stress effects, induced by free radical generator 2-methyl-1,4-naphthoquinone (menadione, MQ), in neuroblastoma SH-SY5Y cells. Several oxidative stress-related parameters were evaluated to measure the protection for mitochondrial integrity, such as mitochondrial superoxide production, mitochondrial activity (MTT), reactive oxygen species (ROS) and live-cell imaging. A significant increase in the ROS level and mitochondrial superoxide production were measured after MQ treatment, however, a subsequent treatment with picein was able to mitigate this effect by decreasing their levels. Additionally, the mitochondrial activity was significantly decreased by MQ exposure, but a follow-up treatment with picein recovered the normal metabolic activity. In conclusion, the presented results demonstrate that picein can significantly reduce the level of MQ-induced oxidative stress on mitochondria, and thereby plays a role as a potent neuroprotectant.
ApoE Genotype-Dependent Response to Antioxidant and Exercise Interventions on Brain Function
Authors: Chaudhari Kiran , Wong Jessica M. , Vann Philip H. , Como Tori , O’Bryant Sid E. , Sumien Nathalie
Published: June 2020

This study determined whether antioxidant supplementation is a viable complement to exercise regimens in improving cognitive and motor performance in a mouse model of Alzheimer’s disease risk. Starting at 12 months of age, separate groups of male and female mice expressing human Apolipoprotein E3 (GFAP-ApoE3) or E4 (GFAP-ApoE4) were fed either a control diet or a diet supplemented with vitamins E and C. The mice were further separated into a sedentary group or a group that followed a daily exercise regimen. After 8 weeks on the treatments, the mice were administered a battery of functional tests including tests to measure reflex and motor, cognitive, and affective function while remaining on their treatment. Subsequently, plasma inflammatory markers and catalase activity in brain regions were measured. Overall, the GFAP-ApoE4 mice exhibited poorer motor function and spatial learning and memory. The treatments improved balance, learning, and cognitive flexibility in the GFAP-ApoE3 mice and overall the GFAP-ApoE4 mice were not responsive. The addition of antioxidants to supplement a training regimen only provided further benefits to the active avoidance task, and there was no antagonistic interaction between the two interventions. These outcomes are indicative that there is a window of opportunity for treatment and that genotype plays an important role in response to interventions.
Characterization of Optically-Reconfigurable Metasurfaces by a Free Space Microwave Bistatic Technique
Authors: Krraoui Houssemeddine , Tripon-Canseliet Charlotte , Maksimovic Ivan , Varault Stefan , Pillet Gregoire , Maci Stefano , Chazelas Jean
Published: June 2020

Microwave performance extraction of optically-controlled squared frequency-selective surface (FSS) structures printed on highly resistive (HR) silicon substrate are presented, from a innovative bistatic microwave photonic characterization technique operating in the 40 to 60 GHz frequency range, commonly used for radar cross section (RCS) measurements. According to typical physical photon absorption phenomenon occurring in photoconductive materials, these structures demonstrate experimentally a bandpass filtering frequency response cancellation through reflection coefficient measurements, under specific incident collective illumination in the Near-infrared region (NIR). This behaviour is attributed to their microwave surface impedance modification accordingly to the incident optical power, allowing ultrafast reconfigurability of such devices by optics
Listeners Sensitivity to Different Locations of Diffusive Surfaces in Performance Spaces: The Case of a Shoebox Concert Hall
Authors: Shtrepi Louena , Di Blasio Sonja , Astolfi Arianna
Published: June 2020

Diffusive surfaces are considered as one of the most challenging aspects to deal with in the acoustic design of concert halls. However, the acoustic effects that these surface locations have on the objective acoustic parameters and on sound perception have not yet been fully understood. Therefore, the effects of these surfaces on the acoustic design parameters have been investigated in a real shoebox concert hall with variable acoustics (Espace de Projection, IRCAM, Paris, France). Acoustic measurements have been carried out in six hall configurations by varying the location of the diffusive surfaces over the front, mid, and rear part of the lateral walls, while the other surfaces have been maintained absorptive or reflective. Moreover, two reference conditions, that is, fully absorptive and reflective boundaries of the hall have been tested. Measurements have been carried out at different positions in the hall, using an artificial head and an array of omnidirectional microphones. Conventional ISO 3382 objective acoustic parameters have been evaluated in all conditions. The results showed that the values of these parameters do not vary significantly with the diffusive surface location. Moreover, a subjective investigation performed by using the ABX method with auralizations at two listening positions revealed that listeners are not sensitive to the diffusive surface location variations even when front-rear asymmetric conditions are compared. However, some of them reported perceived differences relying on reverberance, coloration, and spaciousness.
Characteristics of DSD Bulk Parameters: Implication for Radar Rain Retrieval
Authors: Liao Liang , Meneghini Robert , Iguchi Toshio , Tokay Ali
Published: June 2020

With the use of 213,456 one-minute measured data of droplet-size distribution (DSD) of rain collected during several National Aeronautics and Space Administration (NASA)-sponsored field campaigns, the relationships between rainfall rate R, mass-weighted diameter Dm and normalized intercept parameter Nw of the gamma DSD are studied. It is found, based on the simulations of the gamma DSD model, that R, Dm and Nw are closely interrelated, and that the ratio of R to Nw is solely a function of Dm, independent of the shape factor μ of the gamma distribution. Furthermore, the model-produced ratio agrees well with those from the DSD data. When a power-law equation is applied to fit the model data, we have: R = aN w D m b , where a = 1.588 × 10 − 4 , b = 4.706 . Analysis of two-parameter relationships such as R–Dm, Nw–R and Nw–Dm reveals that R and Dm are moderately correlated while Nw and Dm are negatively correlated. Nw and R, however, are uncorrelated. The gamma DSD model also reveals that variation of R–Dm relation is caused primarily by Nw. For the application of the Ku- and Ka-band dual-frequency radar for the retrieval of the DSD bulk parameters as well as the specific radar attenuations, the study is carried out to relate the dual-frequency radar reflectivity factors to the DSD and attenuation parameters.
Robust Decadal Hydroclimate Predictions for Northern Italy Based on a Twofold Statistical Approach
Authors: Rubinetti Sara , Taricco Carla , Alessio Silvia , Rubino Angelo , Bizzarri Ilaria , Zanchettin Davide
Published: June 2020

The Mediterranean area belongs to the regions most exposed to hydroclimatic changes, with a likely increase in frequency and duration of droughts in the last decades. However, many climate records like, e.g., North Italian precipitation and river discharge records, indicate that significant decadal variability is often superposed or even dominates long-term hydrological trends. The capability to accurately predict such decadal changes is, therefore, of utmost environmental and social importance. Here, we present a twofold decadal forecast of Po River (Northern Italy) discharge obtained with a statistical approach consisting of the separate application and cross-validation of autoregressive models and neural networks. Both methods are applied to each significant variability component extracted from the raw discharge time series using Singular Spectrum Analysis, and the final forecast is obtained by merging the predictions of the individual components. The obtained 25-year forecasts robustly indicate a prominent dry period in the late 2020s/early 2030s. Our prediction provides information of great value for hydrological management, and a target for current and future near-term numerical hydrological predictions.
The Many Faces of Matrix Metalloproteinase-7 in Kidney Diseases
Authors: Liu Zhao , Tan Roderick J. , Liu Youhua
Published: June 2020

Matrix metalloproteinase-7 (MMP-7) is a secreted zinc-dependent endopeptidase that is implicated in regulating kidney homeostasis and diseases. MMP-7 is produced as an inactive zymogen, and proteolytic cleavage is required for its activation. MMP-7 is barely expressed in normal adult kidney but upregulated in acute kidney injury (AKI) and chronic kidney disease (CKD). The expression of MMP-7 is transcriptionally regulated by Wnt/β-catenin and other cues. As a secreted protein, MMP-7 is present and increased in the urine of patients, and its levels serve as a noninvasive biomarker for predicting AKI prognosis and monitoring CKD progression. Apart from degrading components of the extracellular matrix, MMP-7 also cleaves a wide range of substrates, such as E-cadherin, Fas ligand, and nephrin. As such, it plays an essential role in regulating many cellular processes, such as cell proliferation, apoptosis, epithelial-mesenchymal transition, and podocyte injury. The function of MMP-7 in kidney diseases is complex and context-dependent. It protects against AKI by priming tubular cells for survival and regeneration but promotes kidney fibrosis and CKD progression. MMP-7 also impairs podocyte integrity and induces proteinuria. In this review, we summarized recent advances in our understanding of the regulation, role, and mechanisms of MMP-7 in the pathogenesis of kidney diseases. We also discussed the potential of MMP-7 as a biomarker and therapeutic target in a clinical setting.
Prolyl Endopeptidase-Like Facilitates the α-Synuclein Aggregation Seeding, and This Effect Is Reverted by Serine Peptidase Inhibitor PMSF
Authors: Santos Gabriel S. , Oyadomari William Y. , Carvalho Elizangela A. , Torquato Ricardo S. , Oliveira Vitor
Published: June 2020

The aggregation of α-synuclein (α-Syn) is a characteristic of Parkinson’s disease (PD). α-Syn oligomerization/aggregation is accelerated by the serine peptidase, prolyl oligopeptidase (POP). Factors that affect POP conformation, including most of its inhibitors and an impairing mutation in its active site, influence the acceleration of α-Syn aggregation resulting from the interaction of these proteins. It is noteworthy, however, that α-Syn is not cleaved by POP. Prolyl endopeptidase-like (PREPL) protein is structurally related to the serine peptidases belonging to the POP family. Based on the α-Syn–POP studies and knowing that PREPL may contribute to the regulation of synaptic vesicle exocytosis, when this protein can encounter α-Syn, we investigated the α-Syn–PREPL interaction. The binding of these two human proteins was observed with an apparent affinity constant of about 5.7 μM and, as in the α-Syn assays with POP, the presence of PREPL accelerated the oligomerization/aggregation events, with no α-Syn cleavage. Furthermore, despite this lack of hydrolytic cleavage, the serine peptidase active site inhibitor phenylmethylsulfonyl fluoride (PMSF) abolished the enhancement of the α-Syn aggregation by PREPL. Therefore, given the attention to POP inhibitors as potential drugs to treat synucleinopathies, the present data point to PREPL as another potential target to be explored for this purpose.
Photocatalytic Nanofiltration Membrane Using Zr-MOF/GO Nanocomposite with High-Flux and Anti-Fouling Properties
Authors: Heu Rina , Ateia Mohamed , Yoshimura Chihiro
Published: June 2020

Photocatalytic nanofiltration (NF) membranes with enhanced flux and anti-fouling properties were prepared from a layered in situ nanocomposite of metal organic framework (i.e., UiO-66) and graphene oxide (UiO-66_GO) on a polyamide NF membrane using a pressure-assisted self-assembly method. For filtering pure water and humic acid, the composite membrane with a 10% UiO-66_GO loading (UiO-66_GO/NF-10%) showed a higher water flux (up to 63 kg/m2 h bar), flux recovery (80%), and total fouling resistance (33%) than the pristine NF membrane. Physical and chemical characterization revealed that this performance was attributed to improvements in hydrophilicity, porosity, surface smoothness, and charge repulsion. The UiO-66_GO/NF-10% composite membrane exhibited better physical stability with a relatively low mass loss (8.64%) after five washes than the membranes with mass loadings of 5 and 15 wt%. Furthermore, the UiO-66_GO/NF-10% composite membrane exhibited considerable photocatalytic activity under ultraviolet (UV) irradiation (bandgap: 3.45 eV), which reduced irreversible fouling from 20.7% to 2.4% and increased flux recovery to 98%. This study demonstrated that surface modification with the UiO-66_GO nanocomposite produced a high-flux anti-fouling photocatalytic NF membrane, which is promising for water purification.
Vertical Guided Bone Augmentation Using Titanium Mesh Domes Coated with Natural Latex Extracted from Hevea brasiliensis
Authors: Pons Diego K. , Eleuterio Rachel G. , Paiva Fábio F. G. , Holgado Leandro A. , Marques Leonardo , Consolaro Alberto , Graeff Carlos F. O. , Baffa Oswaldo , Kinoshita Angela
Published: June 2020

The subject of this work is the evaluation of the use of titanium mesh domes coated with latex extracted from Hevea brasiliensis to promote vertical guided bone augmentation (GBA), above the normal limits of the skeleton. Twenty-four New Zealand rabbits were used, in which a circular groove of eight millimeters in diameter and nine holes in the internal region reaching the medulla were made with a trephine drill, in the calvaria. The dome, four millimeters in height, was fixed above this defect. The animals were divided into four groups (N = 6). The first (control) received a titanium dome not covered by the periosteum, and the second received a titanium dome that was covered by the periosteum. For the third, a dome with a latex coating was used and was not covered by the periosteum, and for the fourth, a titanium dome with a latex coating was used and was covered by the periosteum. After 90 days, the animals were euthanized. Computerized tomography imaging demonstrated that vertical bone augmentation was achieved in the groups with titanium domes coated with latex. Microscopic evaluation showed that there were no differences between the control group and Group 2, or between Groups 3 and 4. The other comparisons showed statistically significant differences (p < 0.05, ANOVA-Tukey).
Accuracy of a Semi-Quantitative Ultrasound Method to Determine Liver Fat Infiltration in Early Adulthood
Authors: Ibacahe Camila , Correa-Burrows Paulina , Burrows Raquel , Barrera Gladys , Kim Elissa , Hirsch Sandra , Jofré Boris , Blanco Estela , Gahagan Sheila , Bunout Daniel
Published: June 2020

An inexpensive and simple method to determine non-alcoholic fatty liver disease (NAFLD) is the abdominal ultrasound, but there are still doubts about its accuracy. We assessed the precision of a semi-quantitative ultrasound method to determine liver fat infiltration, using magnetic resonance spectroscopy (MRS) as the reference. The study was conducted in youths from an ongoing cohort study. Clinical validation was performed, using receiver operating characteristic analysis, in n = 60 participants (22.6y; 50% males). Abdominal ultrasound was carried out with liver brightness (score 0–3), diaphragm attenuation (0–2) and liver vessel blurring (0–1) scored by two observers. Liver fat was estimated using MRS. Then, analytical validation was conducted in the remaining participants (n = 555; 22.7y; 51% males) using effects size estimates. An ultrasound score ≥4.0 had the highest sensitivity (78%) and specificity (85%) for NAFLD diagnosis. An area under the curve of 86% denotes a good diagnostic performance of the test, whereas a Kappa of 0.63 suggests substantial agreement of ultrasound vs. MRS. The analytical validation showed that participants having NAFLD according to ultrasound had an unhealthier cardiometabolic profile than participants without the condition. Abdominal ultrasound, combined with a semi-quantitative score system, is a reliable method to determine liver fat infiltration in young adults and should be encouraged whenever MRS is unavailable.
Low-Altitude Terrain-Following Flight Planning for Multirotors
Authors: Melita Carmelo Donato , Guastella Dario Calogero , Cantelli Luciano , Di Marco Giuseppe , Minio Irene , Muscato Giovanni
Published: June 2020

Surveying with unmanned aerial vehicles flying close to the terrain is crucial for the collection of details that are not visible when flying at higher altitudes. This type of missions can be applied in several scenarios such as search and rescue, precision agriculture, and environmental monitoring, to name a few. We present a strategy for the generation of low-altitude trajectories for terrain following. The trajectory is generated taking into account the morphology of the area of interest, represented as a georeferenced Digital Surface Model (DSM), while ensuring a safe separation from any obstacle. The surface model of the scenario is created by using a UAV-based photogrammetry software, which processes the images acquired during a preliminary mission at high altitude. The solution was developed, tested, and verified both in simulation and in real scenarios with a multirotor equipped with low-cost sensing. The experimental results proved the validity of the generation of trajectories at altitudes lower than most of the works available in the literature. The images acquired during the low-altitude mission were processed to obtain a high-resolution reconstruction of the area as a representative application result.
Sharp Second-Order Pointwise Asymptotics for Lossless Compression with Side Information
Authors: Gavalakis Lampros , Kontoyiannis Ioannis
Published: June 2020

The problem of determining the best achievable performance of arbitrary lossless compression algorithms is examined, when correlated side information is available at both the encoder and decoder. For arbitrary source-side information pairs, the conditional information density is shown to provide a sharp asymptotic lower bound for the description lengths achieved by an arbitrary sequence of compressors. This implies that for ergodic source-side information pairs, the conditional entropy rate is the best achievable asymptotic lower bound to the rate, not just in expectation but with probability one. Under appropriate mixing conditions, a central limit theorem and a law of the iterated logarithm are proved, describing the inevitable fluctuations of the second-order asymptotically best possible rate. An idealised version of Lempel-Ziv coding with side information is shown to be universally first- and second-order asymptotically optimal, under the same conditions. These results are in part based on a new almost-sure invariance principle for the conditional information density, which may be of independent interest.
Optimization Considerations for Short Channel Poly-Si 1T-DRAM
Authors: Yoo Songyi , Sun Woo-Kyung , Shin Hyungsoon
Published: June 2020

Capacitorless one-transistor dynamic random-access memory cells that use a polysilicon body (poly-Si 1T-DRAM) have been studied to overcome the scaling issues of conventional one-transistor one-capacitor dynamic random-access memory (1T-1C DRAM). Generally, when the gate length of a silicon-on-insulator (SOI) structure metal-oxide-silicon field-effect transistor (MOSFET) is reduced, its body thickness is reduced in order to suppress the short-channel effects (SCEs). TCAD device simulations were used to investigate the transient performance differences between thin and thick-body poly-Si DRAMs to determine whether reduced body thickness is also appropriate for those devices. Analysis of the simulation results revealed that operating bias conditions are as important as body thickness in 1T-DRAM operation. Since a thick-body device has more trapped hole charge in its grain boundary (GB) than a thin-body device in both the “0” and “1” states, the transient performance of a thick-body device is better than a thin-body device regardless of the Write”1” drain voltage. We also determined that the SCEs in the memory cells can be improved by lowering the Write”1” drain voltage. We conclude that an optimization method for the body thickness and voltage conditions that considers both the cell’s SCEs and its transient performance is necessary for its development and application.
Predictions of Rock Temperature Evolution at the Lahendong Geothermal Field by Coupled Numerical Model with Discrete Fracture Model Scheme
Authors: Qarinur Muhammad , Ogata Sho , Kinoshita Naoki , Yasuhara Hideaki
Published: June 2020

The comprehensive exploitation of geothermal fields has an impact on the productivity of the reservoirs. To realize sustainable steam production, changes in the rock temperature need to be predicted and controlled. A coupled thermo-hydro-mechanical (THM) model employing COMSOL Multiphysics was proposed to study the characteristics of heat transfer, fluid flow, and solid deformation at the Lahendong geothermal field, in North Sulawesi, Indonesia. The numerical results were compared with analytical and measured data in order to validate the numerical simulation. Based on the results, the predicted temperatures of the production wells showed significant decrease with the production time. In addition, a reduction in the reservoir temperature leads to lower specific gross electrical power within the production well, which should significantly reduce the sustainability of the power plant.
Exploring DNA Methylation Diversity in the Honey Bee Brain by Ultra-Deep Amplicon Sequencing
Authors: Kucharski Robert , Maleszka Ryszard
Published: June 2020

Understanding methylation dynamics in organs or tissues containing many different cell types is a challenging task that cannot be efficiently addressed by the low-depth bisulphite sequencing of DNA extracted from such sources. Here we explored the feasibility of ultra-deep bisulphite sequencing of long amplicons to reveal the brain methylation patterns in three selected honey bee genes analysed across five distinct conditions on the Illumina MiSeq platform. By combing 15 libraries in one run we achieved a very high sequencing depth of 240,000–340,000 reads per amplicon, suggesting that most of the cell types in the honey bee brain, containing approximately 1 million neurons, are represented in this dataset. We found a small number of gene-specific patterns for each condition in individuals of different ages and performing distinct tasks with 80–90% of those were represented by no more than a dozen patterns. One possibility is that such a small number of frequent patterns is the result of differentially methylated epialleles, whereas the rare and less frequent patterns reflect activity-dependent modifications. The condition-specific methylation differences within each gene appear to be position-dependent with some CpGs showing significant changes and others remaining stable in a methylated or non-methylated state. Interestingly, no significant loss of methylation was detected in very old individuals. Our findings imply that these diverse patterns represent a special challenge in the analyses of DNA methylation in complex tissues and organs that cannot be investigated by low-depth genome-wide bisulphite sequencing. We conclude that ultra-deep sequencing of gene-specific amplicons combined with genotyping of differentially methylated epialleles is an effective way to facilitate more advanced neuro-epigenomic studies in honey bees and other insects.
Identification of QTLs for Resistance to Fusarium Head Blight Using a Doubled Haploid Population Derived from Southeastern United States Soft Red Winter Wheat Varieties AGS 2060 and AGS 2035
Authors: Castro Aviles Alejandro , Alan Harrison Stephen , Joseph Arceneaux Kelly , Brown-Guidera Gina , Esten Mason Richard , Baisakh Niranjan
Published: June 2020

Fusarium head blight (FHB), caused primarily by the fungus Fusarium graminearum, is one of the most damaging diseases of wheat, causing significant loss of yield and quality worldwide. Warm and wet conditions during flowering, a lack of resistant wheat varieties, and high inoculum pressure from corn stubble contribute to frequent FHB epidemics in the southern United States. The soft red winter wheat variety AGS 2060 is moderately susceptible (as opposed to susceptible) to FHB and regularly found in pedigrees of resistant breeding lines. AGS 2060 does not carry any known resistance genes or quantitative trait loci (QTL). A QTL mapping study was conducted to determine the location and genetic effect of its resistance using a doubled haploid mapping population produced from a cross between wheat varieties AGS 2060 and AGS 2035 (FHB susceptible). The population was genotyped using the Illumina iSelect single nucleotide polymorphism (SNP) array for wheat and phenotyped in Baton Rouge and Winnsboro, Louisiana and Newport, Arkansas in 2018 and 2019. The effect of genotype was significant for Fusarium damaged kernels (FDK) and deoxynivalenol (DON) content across all locations and years, indicating genetic variation in the population. The study detected 13 QTLs (one each on chromosome 1A, 1B, 1D, 2A, 2B, 6A, 6B, 7A, and 7B, and two each on 5A and 5B) responsible for the reduction of FDK and/or DON. Of these, nine QTLs for FHB resistance were identified in Winnsboro, Louisiana, in 2019. QTLs on chromosomes 2A and 7A could be valuable sources of resistance to both DON and FDK over several environments and were likely the best candidates for use in marker-assisted selection. Consistently expressed QTLs on chromosomes 5A, 6B, and 7A were potentially newly identified sources of resistance to FHB in soft red winter wheat.
Geology of the Epicentral Area of the November 23, 1980 Earthquake (Irpinia, Italy): New Stratigraphical, Structural and Petrological Constrains
Authors: Matano Fabio , Di Nocera Silvio , Criniti Sara , Critelli Salvatore
Published: June 2020

The geology of the epicentral area of the 1980 earthquake (Irpinia-Lucania, Italy) is described with new stratigraphic, petrographic and structural data. Subsurface geological data have been collected during the studies for the excavation works of the Pavoncelli bis hydraulic tunnel, developing between Caposele and Conza della Campania in an area that was highly damaged during 1980 earthquake. Our approach includes geological, stratigraphic, structural studies, and petrological analyses of rock samples collected along the tunnel profile and in outcropping sections. Stratigraphic studies and detailed geological and structural mapping were carried out in about 200 km2 wide area. The main units cropping out have been studied and correlated in order to document the effects of tectonic changes during the orogenic evolution on the foreland basin systems and the sandstone detrital modes in this sector of the southern Apennines. The multi-disciplinary and updated datasets have allowed getting new insights on the tectono-stratigraphic evolution and stratigraphic architecture of the southern Apennines foreland basin system and on the structural and stratigraphic relations of Apennines tectonic units and timing of their kinematic evolution. They also allowed to better understand the relationships between internal and external basin units within the Apennine thrust belt and its tectonic evolution.
Physical Activity of Children and Adolescents with Hearing Impairments: A Systematic Review
Authors: Xu Wenhong , Li Chunxiao , Wang Lijuan
Published: June 2020

Physical activity (PA) is important for the development of children and adolescents with hearing impairments (HI). This systematic review aims to summarise the existing literature pertaining to the PA of children and adolescents with HI. A systematic search was conducted on eight major electronic databases. Two reviewers independently screened and selected the returned articles, performed data extraction, assessed methodological quality and synthesised the data using an inductive approach. A total of 15 articles consisting of 14 survey studies and one single-subject intervention study met the inclusion criteria. These studies had good to excellent methodological quality. Participants with HI showed lower levels of participation in PA than participants without disabilities, but they were more physically active than those with other types of disabilities. Amongst the 12 PA correlates identified (i.e., gender, age, mother’s education and social cognitive constructs), only gender was a relatively consistent determinant, and boys are significantly more physically active than girls. Additional studies are needed to confirm the determinants of the PA in children and adolescents with HI to provide strong evidence for the development and implementation of PA interventions for this target group.
Addressing Delusions in Women and Men with Delusional Disorder: Key Points for Clinical Management
Authors: González-Rodríguez Alexandre , Seeman Mary V.
Published: June 2020

Delusional disorders (DD) are difficult conditions for health professionals to treat successfully. They are also difficult for family members to bear. The aim of this narrative review is to select from the clinical literature the psychosocial interventions that appear to work best for these conditions and to see whether similar strategies can be modeled or taught to family members so that tensions at home are reduced. Because the content of men’s and women’s delusions sometimes differ, it has been suggested that optimal interventions for the two sexes may also differ. This review explores three areas: (a) specific treatments for men and women; (b) recommended psychological approaches by health professionals, especially in early encounters with patients with DD; and (c) recommended psychoeducation for families. Findings are that there is no evidence for differentiated psychosocial treatment for men and women with delusional disorder. What is recommended in the literature is to empathically elicit the details of the content of delusions, to address the accompanying emotions rather than the logic of the presented argument, to teach self-soothing techniques, and to monitor behavior with respect to its safety. These recommendations have only been validated in individual patients and families. More rigorous clinical trials need to be conducted.
Autophagy-Related 2 Regulates Chlorophyll Degradation under Abiotic Stress Conditions in Arabidopsis
Authors: Jiang Zhuanzhuan , Zhu Li , Wang Qiuyu , Hou Xin
Published: June 2020

Chloroplasts are extraordinary organelles for photosynthesis and nutrient storage in plants. During leaf senescence or under stress conditions, damaged chloroplasts are degraded and provide nutrients for developing organs. Autophagy is a high-throughput degradation pathway for intracellular material turnover in eukaryotes. Along with chloroplast degradation, chlorophyll, an important component of the photosynthetic machine, is also degraded. However, the chlorophyll degradation pathways under high light intensity and high temperature stress are not well known. Here, we identified and characterized a novel Arabidopsis mutant, sl2 (seedling lethal 2), showing defective chloroplast development and accelerated chlorophyll degradation. Map-based cloning combined with high-throughput sequencing analysis revealed that a 118.6 kb deletion region was associated with the phenotype of the mutant. Complementary experiments confirmed that the loss of function of ATG2 was responsible for accelerating chlorophyll degradation in sl2 mutants. Furthermore, we analyzed chlorophyll degradation under abiotic stress conditions and found that both chloroplast vesiculation and autophagy take part in chlorophyll degradation under high light intensity and high temperature stress. These results enhanced our understanding of chlorophyll degradation under high light intensity and high temperature stress.
Innovative Three-Dimensional Microscopic Analysis of Uremic Growth Plate Discloses Alterations in the Process of Chondrocyte Hypertrophy: Effects of Growth Hormone Treatment
Authors: Fernández-Iglesias Ángela , Fuente Rocío , Gil-Peña Helena , Alonso-Durán Laura , García-Bengoa María , Santos Fernando , López José Manuel
Published: June 2020

Chronic kidney disease (CKD) alters the morphology and function of the growth plate (GP) of long bones by disturbing chondrocyte maturation. GP chondrocytes were analyzed in growth-retarded young rats with CKD induced by adenine intake (AD), control rats fed ad libitum (C) or pair-fed with the AD group (PF), and CKD rats treated with growth hormone (ADGH). In order to study the alterations in the process of GP maturation, we applied a procedure recently described by our group to obtain high-quality three-dimensional images of whole chondrocytes that can be used to analyze quantitative parameters like cytoplasm density, cell volume, and shape. The final chondrocyte volume was found to be decreased in AD rats, but GH treatment was able to normalize it. The pattern of variation in the cell cytoplasm density suggests that uremia could be causing a delay to the beginning of the chondrocyte hypertrophy process. Growth hormone treatment appears to be able to compensate for this disturbance by triggering an early chondrocyte enlargement that may be mediated by Nkcc1 action, an important membrane cotransporter in the GP chondrocyte enlargement.