bluuebunny/crossref_metadata_2025 huggingface dataset:
RUNX Family as a Promising Biomarker and a Therapeutic Target in Bone Cancers: A Review on Its Molecular Mechanism(s) behind Tumorigenesis
Authors: Vimalraj Selvaraj , Sekaran Saravanan
Published: June 2023

The transcription factor runt-related protein (RUNX) family is the major transcription factor responsible for the formation of osteoblasts from bone marrow mesenchymal stem cells, which are involved in bone formation. Accumulating evidence implicates the RUNX family for its role in tumor biology and cancer progression. The RUNX family has been linked to osteosarcoma via its regulation of many tumorigenicity-related factors. In the regulatory network of cancers, with numerous upstream signaling pathways and its potential target molecules downstream, RUNX is a vital molecule. Hence, a pressing need exists to understand the precise process underpinning the occurrence and prognosis of several malignant tumors. Until recently, RUNX has been regarded as one of the therapeutic targets for bone cancer. Therefore, in this review, we have provided insights into various molecular mechanisms behind the tumorigenic role of RUNX in various important cancers. RUNX is anticipated to grow into a novel therapeutic target with the in-depth study of RUNX family-related regulatory processes, aid in the creation of new medications, and enhance clinical efficacy.
The Role of Native T1 and T2 Mapping Times in Identifying PD-L1 Expression and the Histological Subtype of NSCLCs
Authors: Bortolotto Chandra , Messana Gaia , Lo Tito Antonio , Stella Giulia Maria , Pinto Alessandra , Podrecca Chiara , Bellazzi Riccardo , Gerbasi Alessia , Agustoni Francesco , Han Fei , Nickel Marcel Dominik , Zacà Domenico , Filippi Andrea Riccardo , Bottinelli Olivia Maria , Preda Lorenzo
Published: June 2023

We investigated the association of T1/T2 mapping values with programmed death-ligand 1 protein (PD-L1) expression in lung cancer and their potential in distinguishing between different histological subtypes of non-small cell lung cancers (NSCLCs). Thirty-five patients diagnosed with stage III NSCLC from April 2021 to December 2022 were included. Conventional MRI sequences were acquired with a 1.5 T system. Mean T1 and T2 mapping values were computed for six manually traced ROIs on different areas of the tumor. Data were analyzed through RStudio. Correlation between T1/T2 mapping values and PD-L1 expression was studied with a Wilcoxon–Mann–Whitney test. A Kruskal–Wallis test with a post-hoc Dunn test was used to study the correlation between T1/T2 mapping values and the histological subtypes: squamocellular carcinoma (SCC), adenocarcinoma (ADK), and poorly differentiated NSCLC (PD). There was no statistically significant correlation between T1/T2 mapping values and PD-L1 expression in NSCLC. We found statistically significant differences in T1 mapping values between ADK and SCC for the periphery ROI (p-value 0.004), the core ROI (p-value 0.01), and the whole tumor ROI (p-value 0.02). No differences were found concerning the PD NSCLCs.
Peripherally Inserted Central Venous Catheter (PICC) Related Bloodstream Infection in Cancer Patients Treated with Chemotherapy Compared with Noncancer Patients: A Propensity-Score-Matched Analysis
Authors: Larcher Romaric , Barrigah-Benissan Koko , Ory Jerome , Simon Claire , Beregi Jean-Paul , Lavigne Jean-Philippe , Sotto Albert
Published: June 2023

The use of peripherally inserted central catheters (PICCs) has increased in cancer patients. This study aimed to compare the incidence of PICC-related bloodstream infections (PICCR-BSIs) in cancer patients treated with chemotherapy and in noncancer patients. We performed a secondary analysis from a retrospective, single-center, observational cohort. The PICCR-BSI incidence rates in cancer and noncancer patients were compared after 1:1 propensity-score matching. Then, the factors associated with PICCR-BSI were assessed in a Cox model. Among the 721 PICCs (627 patients) included in the analysis, 240 were placed in cancer patients for chemotherapy and 481 in noncancer patients. After propensity-score matching, the PICCR-BSI incidence rate was 2.6 per 1000 catheter days in cancer patients and 1.0 per 1000 catheter days in noncancer patients (p < 0.05). However, after adjusting for variables resulting in an imbalance between groups after propensity-score matching, only the number of PICC lumens was independently associated with PICCR-BSI (adjusted hazard ratio 1.81, 95% confidence interval: 1.01–3.22; p = 0.04). In conclusion, the incidence rate of PICCR-BSI is higher in cancer patients treated with chemotherapy than in noncancer patients, but our results also highlight the importance of limiting the number of PICC lumens in such patients.
The Asymmetric Petasis Borono-Mannich Reaction: Insights on the Last 15 Years
Authors: Marques Carolina , Brandão Pedro
Published: June 2023

The Petasis borono-Mannich reaction, commonly described as the Petasis reaction, was one of the latest famous multicomponent reactions described in the literature. Currently celebrating its 30th anniversary since it was first reported by Petasis and Akritopoulou in 1993, this reaction has emerged as a powerful tool for the synthesis of biologically relevant molecules (such as substituted amines or amino acids), among others. This three-component catalyst-free reaction (the classic model), involving the coupling of an aldehyde, an amine, and a boronic acid, enables the synthesis of polysubstituted amine-containing molecules. Several accounts regarding the catalyst-free version using different carbonyl, amine, and boron-type components have been reported thus far. In contrast, the asymmetric version is still in its infancy since it was first reported in 2007. In this work, we aim to review the asymmetric versions of the Petasis reaction reported over the last 15 years, considering the chiral pool approach (asymmetric induction by one reaction component) and the use of catalysts (organocatalysts, transition-metal catalysts, and others) to access enantiomeric and diastereomeric pure amino-derivatives. Insights regarding the catalyzed Petasis reaction and consequent sustainable synthesis will be highlighted.
MoS2@Au as Label for Sensitive Sandwich-Type Immunoassay of Neuron-Specific Enolase
Authors: Wang Yingying , Wang Huixin , Bai Yaliang , Zhao Guanhui , Zhang Nuo , Zhang Yong , Wang Yaoguang , Chi Hong
Published: June 2023

Neuron-specific enolase (NSE) has gained extensive attention as a reliable target for detecting small cell carcinoma of lungs. In this paper, an electrochemical immunoassay method based on molybdenum disulfide (MoS2) is proposed to detect NSE sensitively. By an in-situ growth method, MoS2 and Au nanoclusters (Au NCs) were composited to form a MoS2@Au nanozyme, and then the secondary antibodies were modified. Primary antibodies were immobilized on amino-reduced graphene oxides to capture NSE. The flower-like MoS2 nanozyme provided abundant sites to load Au NCs and catalyze the decomposition of H2O2, which were beneficial to amplify an amperometric response as well as build up sensitivity. Under optimum conditions, the detection range of this strategy was 0.1 pg·mL−1–10 ng·mL−1 and the limit of detection was 0.05 pg·mL−1. This sensing strategy achieved the prospect of sensitively detecting NSE. Moreover, the prepared electrochemical immunosensor provides a theoretical basis and technical support for the detection of other disease markers.
2021/22 and 2022/23 Post-Pandemic Bronchiolitis Seasons in Two Major Italian Cities: A Prospective Study
Authors: Camporesi Anna , Morello Rosa , Pierucci Ugo Maria , Proli Francesco , Lazzareschi Ilaria , Bersani Giulia , Valentini Piero , Roland Damian , Buonsenso Danilo
Published: June 2023

Objectives: Bronchiolitis remains a major cause of morbidity and mortality in children under 24 months. During the first year of the pandemic, non-pharmacological interventions resulted in a significant reduction of bronchiolitis cases. Early in 2021, a rebound of bronchiolitis was reported with a description of out-of-season outbreaks. In this study, we prospectively evaluated the impact of bronchiolitis in two Italian University centers located in different geographical areas, aiming to compare two post-pandemic bronchiolitis seasons (2021/22 and 2022/23) in terms of severity, outcomes, microbiology and temporal distribution. Methods: This was a bicentric prospective observational cohort study. All consecutive children under 24 months of age assessed in the participating institutions during the specified seasons and receiving a clinical diagnosis of bronchiolitis were included. Results: A total of 900 patients were enrolled. Patients in the second season were globally younger and had comorbidities less often. Temporal distribution changed between the two seasons. Of the patients, 56% were tested for RSV; 60% of these was positive. Patients with RSV were globally younger (3.5 months vs. 4.9, p < 0.001), more often had a need for any kind of respiratory and fluid support and more often needed ward or PICU admission. At the end of the ED visit, 430 patients were discharged home, 372 (41.3%) were admitted to an inpatient ward and 46 (5.1%) to a pediatric intensive care unit. Conclusions: The 2022/23 post-COVID bronchiolitis was mostly similar to that of 2021/22, and was in line with pre-pandemic expectations.
Neurologic Complications of Cancer Immunotherapy
Authors: Alsalem Aseel N. , Scarffe Leslie A. , Briemberg Hannah R. , Aaroe Ashley E. , Harrison Rebecca A.
Published: June 2023

Immunotherapy has revolutionized cancer treatment over the past decade. As it is increasingly introduced into routine clinical practice, immune-related complications have become more frequent. Accurate diagnosis and treatment are essential, with the goal of reduced patient morbidity. This review aims to discuss the various clinical manifestations, diagnosis, treatments, and prognosis of neurologic complications associated with the use of immune checkpoint inhibitors, adoptive T-cell therapies, and T-cell redirecting therapies. We also outline a suggested clinical approach related to the clinical use of these agents.
Artificial Intelligence for Automated DWI/FLAIR Mismatch Assessment on Magnetic Resonance Imaging in Stroke: A Systematic Review
Authors: Offersen Cecilie Mørck , Sørensen Jens , Sheng Kaining , Carlsen Jonathan Frederik , Langkilde Annika Reynberg , Pai Akshay , Truelsen Thomas Clement , Nielsen Michael Bachmann
Published: June 2023

We conducted this Systematic Review to create an overview of the currently existing Artificial Intelligence (AI) methods for Magnetic Resonance Diffusion-Weighted Imaging (DWI)/Fluid-Attenuated Inversion Recovery (FLAIR)—mismatch assessment and to determine how well DWI/FLAIR mismatch algorithms perform compared to domain experts. We searched PubMed Medline, Ovid Embase, Scopus, Web of Science, Cochrane, and IEEE Xplore literature databases for relevant studies published between 1 January 2017 and 20 November 2022, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. We assessed the included studies using the Quality Assessment of Diagnostic Accuracy Studies 2 tool. Five studies fit the scope of this review. The area under the curve ranged from 0.74 to 0.90. The sensitivity and specificity ranged from 0.70 to 0.85 and 0.74 to 0.84, respectively. Negative predictive value, positive predictive value, and accuracy ranged from 0.55 to 0.82, 0.74 to 0.91, and 0.73 to 0.83, respectively. In a binary classification of ±4.5 h from stroke onset, the surveyed AI methods performed equivalent to or even better than domain experts. However, using the relation between time since stroke onset (TSS) and increasing visibility of FLAIR hyperintensity lesions is not recommended for the determination of TSS within the first 4.5 h. An AI algorithm on DWI/FLAIR mismatch assessment focused on treatment eligibility, outcome prediction, and consideration of patient-specific data could potentially increase the proportion of stroke patients with unknown onset who could be treated with thrombolysis.
Four-Dimensional Flow MRI for the Evaluation of Aortic Endovascular Graft: A Pilot Study
Authors: Righini Paolo , Secchi Francesco , Mazzaccaro Daniela , Giese Daniel , Galligani Marina , Avishay Dor , Capra Davide , Monti Caterina Beatrice , Nano Giovanni
Published: June 2023

We aimed to explore the feasibility of 4D flow magnetic resonance imaging (MRI) for patients undergoing thoracic aorta endovascular repair (TEVAR). We retrospectively evaluated ten patients (two female), with a mean (±standard deviation) age of 61 ± 20 years, undergoing MRI for a follow-up after TEVAR. All 4D flow examinations were performed using a 1.5-T system (MAGNETOM Aera, Siemens Healthcare, Erlangen, Germany). In addition to the standard examination protocol, a 4D flow-sensitive 3D spatial-encoding, time-resolved, phase-contrast prototype sequence was acquired. Among our cases, flow evaluation was feasible in all patients, although we observed some artifacts in 3 out of 10 patients. Three individuals displayed a reduced signal within the vessel lumen where the endograft was placed, while others presented with turbulent or increased flow. An aortic endograft did not necessarily hinder the visualization of blood flow through 4D flow sequences, although the graft could generate flow artifacts in some cases. A 4D Flow MRI may represent the ideal tool to follow up on both healthy subjects deemed to be at an increased risk based on their anatomical characteristics or patients submitted to TEVAR for whom a surveillance protocol with computed tomography angiography would be cumbersome and unjustified.
Medical Applications of Molecular Biotechnologies in the Context of Hashimoto’s Thyroiditis
Authors: Trovato Maria , Valenti Andrea
Published: June 2023

Hashimoto’s thyroiditis (HT) is a gender autoimmune disease that is manifested by chronic inflammation of the thyroid. Clinical trial studies (CTSs) use molecular biotechnologies (MB) to approach HT appearance. The aims of this study were to analyze the applications of MB in CTSs carried out in HT populations (HT-CTSs). Further, to evaluate the role of MB in the context of the hygiene hypothesis (HH). From 75 HT-CTSs found at clinicaltrials.gov web place, forty-five were considered for this investigation. Finally, six HT-CTSs were reported as molecular HT-CTSs (mHT-CTSs) because these were planning to utilize MB. Two of mHT-CTSs were programmed on the French population to isolate DNA viral sequences. Blood, urine, and thyroid tissue biospecimens were analyzed to pick out the parvo and polyoma viruses. Two mHT-CTSs carried out in China aimed to identify oral and fecal microbiotas by measuring PCR sequencing of the 16S rRNA gene. Two mHT-CTSs were programmed in the USA and Greece, respectively, for interception of DNA polymorphisms to associate with genetic susceptibility to HT. In conclusion, MB are mainly employed in HT-CTSs for infective pathogenesis and genetic fingerprinting of HT. Furthermore, MB do not provide evidence of HH; however, they are useful for providing direct evidence of the presence of viruses.
Young Generations’ Perception of the Role of Deadwood in Forests: Comparison between Italy and Türkiye
Authors: Paletto Alessandro , Bayraktar Selim , Becagli Claudia , De Meo Isabella
Published: June 2023

The recreational value of a forest is related to the degree of naturalness that can be measured by considering qualitative and quantitative characteristics of deadwood and habitat trees. Forest user groups’ knowledge and opinions towards forest naturalness can support decision-makers in defining strategies. Among user groups, the literature shows a knowledge gap regarding the new generations’ perceptions and opinions related to biodiversity conservation. The aim of this study is to investigate young generations’ perception and preferences towards deadwood in forests. A questionnaire was developed and administrated online—in Italy and Türkiye—to a sample of university students under 25 years old. A total of 407 questionnaires (187 in Italy and 220 in Türkiye) were collected and processed. The results show that students from both countries have a high level of knowledge of deadwood and its role in forests. Most respondents perceive standing dead trees in forests to be aesthetically negative, while large lying deadwood is perceived as aesthetically positive. The attention of young generations towards issues related to biodiversity conservation is confirmed by the significance given in both countries to the management strategy that closely aligns with natural processes of mortality in undisturbed forests, where deadwood is allowed to remain without removal.
Being about It: Engaging Liberatory Educational Praxis
Authors: Richardson Chatee Omisade
Published: June 2023

Engaging in liberatory praxis at times means operating outside of set or limiting boundaries. As such, this article is written in the first person to highlight both a praxis of freedom (outside of the status quo) and to show impact. It pays homage to elder educators in the process of (re)membering those who have paved the way for liberatory praxis and influence my personal educational philosophy presented here for guidance. Student voices are amplified in the process of highlighting ways that (re)searching, (re)visioning, (re)cognizing, (re)presenting, and (re)claiming are actualized to engender educational freedom across curricular content in a higher educational setting.
Effect of the Particle Size Distribution of Irregular Al Powder on Properties of Parts for Electronics Fabricated by Binder Jetting
Authors: Marczyk Joanna , Hebda Marek
Published: June 2023

The present work analyzed the influence of the particle size of irregular aluminum powder on the properties of Binder-Jetting-printed parts, which can be used as electronic components. Powders of various particle sizes as well as blends in the ratio of 73–27 wt.% or 27–73 wt.% of coarse to fine powder particles were used. The parts were printed with constant parameters, such as a layer thickness of 120 µm, roller traverse speed of 10 mm/s, and binder saturation of 80%. For parts made of individual blends, analysis of the XRD, density, porosity, surface roughness, and dimensional changes in X, Y, and Z axes after the sintering process was conducted. The results confirmed the trend of smoothing the surface of 3D-printed parts with a reduction in the size of the powder particles used. The best results in terms of surface roughness were obtained for powder in which coarse particles (73%) had 50 µm and fine particles (27%) had 20 µm. However, the incorporation of coarser particles in an amount of 27 wt.% (AL160) to the fine-grained powder base (ALC100) allowed for the obtaining of details with higher density, lower total porosity, and relatively low surface roughness. The combination of these two powder particle sizes allowed the fine powder to fill the voids between the larger particles, resulting in properties that represent an excellent relationship between density, porosity, and surface quality. The research results indicate that the three-dimensional parts produced by Binder Jetting technology, through the phenomenal thermal conductivity of aluminum, can be successfully used as electronic components, such as heat sinks or transistor housings.
Theoretical DFT Investigation of Structure and Electronic Properties of η5-Cyclopentadienyl Half-Sandwich Organochalcogenide Complexes
Authors: Oyeniyi G. T. , Melchakova Iu. A. , Polyutov S. P. , Avramov P. V.
Published: June 2023

For the first time, an extensive theoretical comparative study of the electronic structure and spectra of the η5-cyclopentadienyl half-sandwich [(Cp)(EPh3)], E = Se, Te) organochalcogenides was carried out using direct space electronic structure calculations within hybrid, meta, and meta-hybrid DFT GGA functionals coupled with double-ζ polarized 6-31G* and correlation-consistent triple-zeta cc-pVTZ-pp basis sets. The absence of covalent bonding between the cyclopentadienyl (Cp) ligands and Te/Se coordination centers was revealed. It was found that the chalcogens are partially positively charged and Cp ligands are partially negatively charged, which directly indicates a visible ionic contribution to Te/Se-Cp chemical bonding. Simulated UV–Vis absorption spectra show that all complexes have a UV-active nature, with a considerable shift in their visible light absorption due to the addition of methyl groups. The highest occupied molecular orbitals exhibit π-bonding between the Te/Se centers and Cp rings, although the majority of the orbital density is localized inside the Cp π-system. The presence of the chalcogen atoms and the extension of π-bonds across the chalcogen-ligand interface make the species promising for advanced photovoltaic and light-emitting applications.
Study of Particle Size Measurement by the Extinction Method in Flame
Authors: Xiang Hengsheng , Cheng Bo , Zhang Chengfei , Qiao Wensheng
Published: June 2023

The laser extinction method (LEM) is particularly suitable for measuring particle sizes in flames because this method, which is based on the Beer–Lambert law, is non-intrusive and easy to implement. In the LEM, the interpretation of the extinction data is usually developed under the assumption that light extinction due to scattering is a result of the superposition of single scattering by individual particles; however, this could be violated for flames with dense concentrations of particles in which multiple scattering could occur. Quantifying the effect of multiple scattering under general conditions is still a formidable problem. In this work, we carried out a series of careful measurements of the laser extinction using standard particles of various known sizes, number densities and optical path lengths, all under the condition that the acceptance angle of the detector was limited to nearly zero. Combined with a four-flux model, we quantitatively analyzed the effect of multiple scattering on the size measurement using the LEM. The results show that the effect of multiple scattering could be ignored when the optical thickness is less than two under strict restrictions on the detector acceptance angle. Guided by this, the size distribution of an alumina (Al2O3) particle sample was measured by the LEM with dual wavelengths. Parameterized distributions were solved with the help of graph plotting, and the results compared well with the measurement from the Malvern particle size analyzer. The same method was then used to measure the particle size distribution in the plume of a solid rocket motor (SRM). The use of an off-axis parabolic mirror in the experimental setup could suppress the jitter of light passing through the SRM plume, and the particle size in the plume of the measured SRM was in the order of microns.
Optimization of Process Parameters for Pellet Production from Corn Stalk Rinds Using Box–Behnken Design
Authors: Liu Dan , Teng Da , Zhu Yan , Wang Xingde , Wang Hanyang
Published: June 2023

In the current study, corn stalk rinds were used as feedstock for the production of solid-fuel pellets. In an effort to comprehensively analyze the effects of different operational parameters on the physical properties of pellets, response surface methodology (RSM) was employed in conjunction with a Box–Behnken experimental design (BBD). By assessing multiple variables simultaneously and examining their interactions, BBD facilitates the development of a reliable response model that can predict how changes in independent variables will impact response variables. The recorded responses included relaxed density, mechanical durability, and compressive strength. Based on the results, greater R2 values of 0.9467, 0.8669, and 0.9196, could be, respectively, attained for the quadratic regression models. The analysis of variance revealed that all independent variables had significant effects on the responses. The optimal processing condition for the pellets was established by determining the ideal combination of operational parameters. The process entailed the choice of a particle dimension measuring 0.5 mm, a moisture level of 11.35%, the application of heat at 125.7 °C on the die, and the utilization of a molding pressure of 154.2 MPa. Based on these factors, the predicted response values were determined to be 1639.61 kg/m3 for relaxed density, 97.95% for mechanical durability, and 10.18 MPa for compressive strength. The values obtained experimentally under the optimized conditions were similar to the predicted values with a desirability value of 1.00.
A Two-Step Site Selection Concept for Underground Pumped Hydroelectric Energy Storage and Potential Estimation of Coal Mines in Henan Province
Authors: Chen Qianjun , Hou Zhengmeng , Wu Xuning , Zhang Shengyou , Sun Wei , Fang Yanli , Wu Lin , Huang Liangchao , Zhang Tian
Published: June 2023

In the context of carbon neutrality, the phase-out of coal from the energy structure has resulted in numerous old coal mines that possess abundant underground space resources suitable for underground pumped hydroelectric energy storage (UPHES). Site selection and estimation of potential are critical to the planning and implementation of UPHES in old coal mines. This paper introduces a two-step site selection concept, including a screening assessment followed by a comprehensive assessment, to determine suitable locations for UPHES. The screening indicators in the screening assessment comprise geological features, mine water disasters, and minimum installed capacity, while the analytic hierarchy process (AHP) is applied in the comprehensive assessment. Additionally, coal mines in Henan Province are preliminarily screened through the screening assessment and the potential for UPHES is thoroughly investigated. The estimated volume of the drifts and shafts in old coal mines is approximately 1.35 × 107 m3, while in producing coal mines, it is around 2.96 × 107 m3. Furthermore, the corresponding annual potential for UPHES is 1468.9 GWh and 3226.3 GWh, respectively. By consuming surplus wind and solar power, UPHES is able to reduce 4.68 × 105 tonnes of carbon dioxide (CO2) emissions. The study provides preliminary guidance for policy-makers in developing UPHES in old coal mines.
Horse Manure and Lignocellulosic Biomass Characterization as Methane Production Substrates
Authors: Naji Amar , Rechdaoui Sabrina Guérin , Jabagi Elise , Lacroix Carlyne , Azimi Sam , Rocher Vincent
Published: June 2023

This paper aimed to study the value of horse manure through anaerobic digestion. The study involved characterization of different components of horse waste and the evaluation of their biochemical composition, physicochemical characterization and the influence of the composition of horse waste on biochemical methane potential. More specifically, two bedding mixtures were studied: the first one was composed of wheat straw (WS), wood chips (WC) and horse manure (HM) with a volumetric composition of 85%, 14% and 1%, respectively; and the second one was a mixture of WS and HM with a volumetric composition of 99% and 1%, respectively. The analysis was carried out on the two bedding mixtures and on each substrate separately with 406 samples from May 2017 to October 2019. Biochemical methane potential tests conducted on these samples showed that the composition and structure of the substrate influenced the BMP. WS had the highest mono-digestion methane production with 176.1 NmL·gVS−1. The second bedding mixture (99% WS, 1% HM) showed a production of 189.4 NmL·gVS−1 compared to 127 NmL·gVS−1 by bedding mixture 1 (85% WS, 14% WC, 1% HM). The difference was due to a dilution effect on methane production caused by the presence of WC rich in lignin.
Optimal Meshing Degree Performance Analysis in a mmWave FWA 5G Network Deployment
Authors: Gheyas Iffat , Raschella Alessandro , Mackay Michael
Published: June 2023

Fifth-generation technologies have reached a stage where it is now feasible to consider deployments that extend beyond traditional public networks. Central to this process is the application of Fixed Wireless Access (FWA) in 5G Non-public Networks (NPNs) that can utilise a novel combination of radio technologies to deploy an infrastructure on top of 5G NR or entirely from scratch. However, the use of FWA backhaul faces many challenges in relation to the trade-offs for reduced costs and a relatively simple deployment. Specifically, the use of meshed deployments is critical as it provides resilience against a temporary loss of connectivity due to link errors. In this paper, we examine the use of meshing in a FWA backhaul to determine if an optimal trade-off exists between the deployment of more nodes/links to provide multiple paths to the nearest Point of Presence (POP) and the performance of the network. Using a real 5G NPN deployment as a basis, we have conducted a simulated analysis of increasing network densities to determine the optimal configuration. Our results show a clear advantage for meshing in general, but there is also a performance trade-off to consider between overall network throughput and stability.
Assessing Fire Risk Perception in the Vale do Guadiana Natural Park, Portugal
Authors: Andrade Nuno , Couto Flavio T. , Serra Jaime
Published: June 2023

This is an exploratory study aiming to assess the fire risk perception by operators of the Vale do Guadiana Natural Park (PNVG), southern Portugal. To maximize the sample size, a questionnaire survey was distributed among 35 entities with activities in tourism, hunting, and agriculture, as well as among members of PNVG’s co-management commission. For data analysis and interpretation, quantitative and qualitative analyses were used. Survey responses revealed that the entities expressed concern about and made efforts toward the search for and improvement in mitigation strategies in the occurrence of fires. A total of 69.6% of the respondents have knowledge of the occurrence of fires in the region. The qualitative analysis highlights the concern with biodiversity, as well as with the maintenance and cleaning of the PNVG. This study verifies the degree of importance that the tourism sector should give to the impacts caused by fires. The impact of climate change favoring fires was recognized by the entities, as well as the fact that the loss of biodiversity due to fires may have a direct impact on the attractiveness of this tourist destination, indicating the importance of environmental conservation strategies for the region.
The Effects of Grapevine (Vitis vinifera L.) Leaf Extract on Growth Performance, Antioxidant Status, and Immunity of Zebrafish (Danio rerio)
Authors: Hoseinifar Seyed Hossein , Fazelan Zohreh , El-Haroun Ehab , Yousefi Morteza , Yazici Metin , Van Doan Hien , Paolucci Marina
Published: June 2023

An 8-week feeding trial was carried out to evaluate the effects of grapevine (Vitis vinifera) leaf extract (GLE) on the growth, oxidative enzymatic activities, immunity, and expression of antioxidant genes in zebrafish (Danio rerio). Three hundred and sixty zebrafish were supplied and fed with different levels of GLE: 0, 0.5, 1, and 2 g kg−1. The dietary administration of 1 g kg−1 of GLE significantly increased growth parameters in fish. Fish fed diets with GLE showed increased total protein. The total Ig and lysozyme activity significantly changed in the whole-body serum, but not in skin mucus. GLE significantly increased Catalase (CAT), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) activities compared to the control diet. GLE treatments caused a significant decrease in the malondialdehyde (MDA) content. In the skin mucus, only CAT and SOD activities significantly increased. The highest expression of Toll-like receptor-1 (TLR-1) and Tumor Necrosis Factor-α (TNFα) genes was achieved in fish fed 2 g kg−1 of GLE. CAT and SOD gene expressions were significantly higher in fish fed 1 and 2 g kg−1 of GLE. GPx gene expression was significantly higher in fish fed 1 g kg−1 of GLE. In conclusion, the results of the present study revealed that GLE affects growth performance and regulates antioxidant and immune gene expression. The determination of the optimum dosage merits further research.
Selenite Removal from Aqueous Solution Using Silica–Iron Oxide Nanocomposite Adsorbents
Authors: Mladin Georgiana , Ciopec Mihaela , Negrea Adina , Duteanu Narcis , Negrea Petru , Svera (m. Ianăşi) Paula , Ianăşi Cătălin
Published: June 2023

In recent years, during industrial development, the expanding discharge of harmful metallic ions from different industrial wastes (such as arsenic, barium, cadmium, chromium, copper, lead, mercury, nickel, selenium, silver, or zinc) into different water bodies has caused serious concern, with one of the problematic elements being represented by selenium (Se) ions. Selenium represents an essential microelement for human life and plays a vital role in human metabolism. In the human body, this element acts as a powerful antioxidant, being able to reduce the risk of the development of some cancers. Selenium is distributed in the environment in the form of selenate (SeO42–) and selenite (SeO32–), which are the result of natural/anthropogenic activities. Experimental data proved that both forms present some toxicity. In this context, in the last decade, only several studies regarding selenium’s removal from aqueous solutions have been conducted. Therefore, in the present study, we aim to use the sol–gel synthesis method to prepare a nanocomposite adsorbent material starting from sodium fluoride, silica, and iron oxide matrices (SiO2/Fe(acac)3/NaF), and to further test it for selenite adsorption. After preparation, the adsorbent material was characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). The mechanism associated with the selenium adsorption process has been established based on kinetic, thermodynamic, and equilibrium studies. Pseudo second order is the kinetic model that best describes the obtained experimental data. Also, from the intraparticle diffusion study, it was observed that with increasing temperature the value of the diffusion constant, Kdiff, also increases. Sips isotherm was found to best describe the experimental data obtained, the maximum adsorption capacity being ~6.00 mg Se(IV) per g of adsorbent material. From a thermodynamic point of view, parameters such as ΔG0, ΔH0, and ΔS0 were evaluated, proving that the process studied is a physical one.
Synthesis of New Hydrogels Involving Acrylic Acid and Acrylamide Grafted Agar-Agar and Their Application in the Removal of Cationic Dyes from Wastewater
Authors: Betraoui Amina , Seddiki Nesrinne , Souag Rafika , Guerfi Nabila , Semlali Abdelhabib , Aouak Taieb , Aliouche Djamel
Published: June 2023

Polyacrylic Acid grafted Agar-agar (AAc-graf-Agar), and polyacrylamide grafted Agar-Agar (AAm-graf-Agar) have been synthesised by free radical polymerisation route initiated by ammonium peroxodisulphate (APS), the grafted polymers were characterised by FTIR, TGA and SEM methods. The swelling properties were studied in deionised water and saline solution at room temperature. The prepared hydrogels were examined by removing cationic methylene blue (MB) dye from the aqueous solution, in which the adsorption kinetics and isotherms models were also investigated. It was found that the pseudo-second-order and Langmuir equations are the most suitable for the different sorption processes. The maximum dye adsorption capacity was 1035.96 mg∙g−1 for AAc-graf-Agar in pH medium 12 and 1015.7 mg∙g−1 for AAm-graf-Agar in neutral pH medium. This indicates that the AAc-graf-Agar hydrogel could be an excellent adsorbent for removing MB from aqueous solutions.
Histone Deacetylases (HDAC) Inhibitor—Valproic Acid Sensitizes Human Melanoma Cells to Dacarbazine and PARP Inhibitor
Authors: Drzewiecka Małgorzata , Gajos-Michniewicz Anna , Hoser Grażyna , Jaśniak Dominika , Barszczewska-Pietraszek Gabriela , Sitarek Przemysław , Czarny Piotr , Piekarski Janusz , Radek Maciej , Czyż Małgorzata , Skorski Tomasz , Śliwiński Tomasz
Published: June 2023

The inhibition of histone deacetylases (HDACs) holds promise as a potential anti-cancer therapy as histone and non-histone protein acetylation is frequently disrupted in cancer, leading to cancer initiation and progression. Additionally, the use of a histone deacetylase inhibitor (HDACi) such as the class I HDAC inhibitor—valproic acid (VPA) has been shown to enhance the effectiveness of DNA-damaging factors, such as cisplatin or radiation. In this study, we found that the use of VPA in combination with talazoparib (BMN-673—PARP1 inhibitor—PARPi) and/or Dacarbazine (DTIC—alkylating agent) resulted in an increased rate of DNA double strand breaks (DSBs) and reduced survival (while not affecting primary melanocytes) and the proliferation of melanoma cells. Furthermore, the pharmacological inhibition of class I HDACs sensitizes melanoma cells to apoptosis following exposure to DTIC and BMN-673. In addition, the inhibition of HDACs causes the sensitization of melanoma cells to DTIV and BMN-673 in melanoma xenografts in vivo. At the mRNA and protein level, the histone deacetylase inhibitor downregulated RAD51 and FANCD2. This study aims to demonstrate that combining an HDACi, alkylating agent and PARPi could potentially enhance the treatment of melanoma, which is commonly recognized as being among the most aggressive malignant tumors. The findings presented here point to a scenario in which HDACs, via enhancing the HR-dependent repair of DSBs created during the processing of DNA lesions, are essential nodes in the resistance of malignant melanoma cells to methylating agent-based therapies.
How Do Midwives View Their Professional Autonomy, Now and in Future?
Authors: Vermeulen Joeri , Fobelets Maaike , Fleming Valerie , Luyben Ans , Stas Lara , Buyl Ronald
Published: June 2023

Background: Internationally, midwives’ professional autonomy is being challenged, resulting in their inability to practice to their full scope of practice. This situation contrasts with the increasing international calls for strengthening the midwifery profession. The aim of this study therefore is to explore Belgian midwives’ views on their current and future autonomy. Methods: An online survey among Belgian midwives was performed. Data were collected and analyzed using a quantitative approach, while quotes from respondents were used to contextualize the quantitative data. Results: Three hundred and twelve midwives from different regions and professional settings in Belgium completed the questionnaire. Eighty-five percentage of respondents believe that they are mostly or completely autonomous. Brussels’ midwives feel the most autonomous, while those in Wallonia feel the least. Primary care midwives feel more autonomous than hospital-based midwives. Older midwives and primary care midwives feel less recognized and respected by other professionals in maternity care. The majority of our respondents believe that in future midwives should be able to work more autonomously in constructive collaboration with other professionals. Conclusion: While Belgian midwives generally rated their own professional autonomy as high, a significant majority of respondents desire more autonomy in future. In addition, our respondents want to be recognized and respected by society and other health professionals in maternity care. It is recommended to prioritize efforts in enhancing midwives’ autonomy, while also addressing the need for increased recognition and respect from society and other maternity care professionals.
Shared Decision-Making to Improve Health-Related Outcomes for Adults with Stroke Disease
Authors: Bajenaru Lidia , Sorici Alexandru , Mocanu Irina Georgiana , Florea Adina Magda , Antochi Florina Anca , Ribigan Athena Cristina
Published: June 2023

Stroke is one of the leading causes of disability and death worldwide, a severe medical condition for which new solutions for prevention, monitoring, and adequate treatment are needed. This paper proposes a SDM framework for the development of innovative and effective solutions based on artificial intelligence in the rehabilitation of stroke patients by empowering patients to make decisions about the use of devices and applications developed in the European project ALAMEDA. To develop a predictive tool for improving disability in stroke patients, key aspects of stroke patient data collection journeys, monitored health parameters, and specific variables covering motor, physical, emotional, cognitive, and sleep status are presented. The proposed SDM model involved the training and consultation of patients, medical staff, carers, and representatives under the name of the Local Community Group. Consultation with LCG members, consists of 11 representative people, physicians, nurses, patients and caregivers, which led to the definition of a methodological framework to investigate the key aspects of monitoring the patient data collection journey for the stroke pilot, and a specific questionnaire to collect stroke patient requirements and preferences. A set of general and specific guidelines specifying the principles by which patients decide to use wearable sensing devices and specific applications resulted from the analysis of the data collected using the questionnaire. The preferences and recommendations collected from LCG members have already been implemented in this stage of ALAMEDA system design and development.
Physiological and Molecular Screening of High Temperature Tolerance in Okra [Abelmoschus esculentus (L.) Moench]
Authors: Hayamanesh Shahnoosh , Trethowan Richard , Mahmood Tariq , Ahmad Nabil , Keitel Claudia
Published: June 2023

Okra is a vegetable crop adapted to summer temperatures, but heat stress has been shown to reduce its growth and productivity. We measured physiological traits of 104 okra genotypes in response to high temperature, augmented by the molecular characterization of selected genotypes to identify parents for crossing. Genotypes were exposed to a short heat shock (45 °C, 4 h) in a controlled environment, followed by the assessment of chlorophyll fluorescence (Fv/Fm, Fv′/Fm′) and stomatal conductance (gs). DNA was isolated from all genotypes using a modified CTAB method with additional PVP and RNase, and the amplification of 8 polymorphic SSR markers was used to generate a dendrogram. This preliminary screening identified 33 polymorphic genotypes with less than 50% genetic similarity and contrasting Fv′/Fm′ and gs responses. More detailed physiological measurements (Fv/FmFv′/Fm′, gs, photosynthesis (A), efficiency of the open reaction centre (ΦPSII), and electrolyte leakage (EL)) were conducted after exposure to 45 °C for 6 h and compared to the control (30 °C). EL did not significantly increase in the heat treatment; in contrast, there were significant genotype and treatment effects observed for fluorescence (Fv/Fm, Fv′/Fm′) and photosynthetic parameters (A, ΦPSII, gs). In conclusion, cell membranes in okra remained unaffected after short periods of heat stress, whereas the ranking of differences of measured physiological traits (∆) between control and heat-treated plants (∆Fv′/Fm′, ∆Fv′/Fm′, ∆A, ∆ΦPSII, ∆gs) was indicative of genotype sensitivity to heat.
Automated Detection of Patients at High Risk of Polypharmacy including Anticholinergic and Sedative Medications
Authors: Shirazibeheshti Amirali , Ettefaghian Alireza , Khanizadeh Farbod , Wilson George , Radwan Tarek , Luca Cristina
Published: June 2023

Ensuring that medicines are prescribed safely is fundamental to the role of healthcare professionals who need to be vigilant about the risks associated with drugs and their interactions with other medicines (polypharmacy). One aspect of preventative healthcare is to use artificial intelligence to identify patients at risk using big data analytics. This will improve patient outcomes by enabling pre-emptive changes to medication on the identified cohort before symptoms present. This paper presents a mean-shift clustering technique used to identify groups of patients at the highest risk of polypharmacy. A weighted anticholinergic risk score and a weighted drug interaction risk score were calculated for each of 300,000 patient records registered with a major regional UK-based healthcare provider. The two measures were input into the mean-shift clustering algorithm and this grouped patients into clusters reflecting different levels of polypharmaceutical risk. Firstly, the results showed that, for most of the data, the average scores are not correlated and, secondly, the high risk outliers have high scores for one measure but not for both. These suggest that any systematic recognition of high-risk groups should consider both anticholinergic and drug–drug interaction risks to avoid missing high-risk patients. The technique was implemented in a healthcare management system and easily and automatically identifies groups at risk far faster than the manual inspection of patient records. This is much less labour-intensive for healthcare professionals who can focus their assessment only on patients within the high-risk group(s), enabling more timely clinical interventions where necessary.
Does the Sound of a Singing Bowl Synchronize Meditational Brainwaves in the Listeners?
Authors: Kim Seong-Chan , Choi Min-Joo
Published: June 2023

This study aims to verify if the beating sound of a singing bowl synchronizes and activates brain waves during listening. The singing bowl used in this experiment produce beats at a frequency of 6.68 Hz, while it decays exponentially and lasts for about 50 s. Brain waves were measured for 5 min in the F3 and F4 regions of seventeen participants (eight males and nine females, average age 25.2) who heard the beating singing bowl sounds. The experimental results showed that the increases (up to ~251%) in the spectral magnitudes of the brain waves were dominant at the beat frequency compared to those of any other clinical brain wave frequency bands. The observed synchronized activation of the brain waves at the beating sound frequency supports that the singing bowl sound may effectively facilitate meditation and relaxation, considering that the beat frequency belongs to the theta wave region which increases in the relaxed meditation state.
Water, Sanitation, and Hygiene Challenges in Informal Settlements in Kampala, Uganda: A Qualitative Study
Authors: Dickson-Gomez Julia , Nyabigambo Agnes , Rudd Abigail , Ssentongo Julius , Kiconco Arthur , Mayega Roy William
Published: June 2023

Diarrhea causes 1.6 million deaths annually, including 525,000 children. Further, chronic diarrhea puts children at risk for mineral deficiencies, malnutrition, and stunting which, in turn, can result in cognitive deficits, poor performance in school, and decreased disease immunity in adulthood. Most diarrhea is caused by water contaminated by fecal matter. Interventions to improve clean water and sanitation can save lives; however, challenges persist in informal settlements. In this study, we explored the views of residents of informal settlements regarding water and sanitation in their communities. Focus group interviews were conducted with residents of 6 informal settlements in Kampala, Uganda (n = 165 people), and 6 key informant interviews were conducted with governmental and nongovernmental organizations that work to improve informal settlements or provide services to them. The results from this study demonstrate that, although these informal settlements had many infrastructure “upgrades” such as latrines and toilets, water taps, wells, and garbage collection and drainage systems, the water, sanitation, and hygiene (WASH) system and its components largely failed due to point-of-use charges of water taps and toilets and the difficulty of emptying cesspits. Our results suggest that WASH must be considered a system and that multiple upgrading efforts are needed for WASH systems to work, including road construction and better oversight of fecal sludge disposal.