bluuebunny/crossref_metadata_2025 huggingface dataset:
Incidence and Trend of Type I and II Endometrial Cancer in Women from Two Population-Based European Cancer Registries (1998–2012)
Authors: Rodríguez-Palacios Daniel Ángel , Colorado-Yohar Sandra M. , Velten Michel , Vaamonde-Martín Ricardo J. , Ballesta Mónica , Chirlaque María-Dolores
Published: March 2022

Endometrial cancer (EC) is the most frequent female genital tract cancer in Europe. This cohort study aimed to determine age-standardised incidence rates and long-term trends of type I and II endometrial cancer in women from population-based cancer registries in the Region of Murcia (Spain) and the Bas-Rhin area (France). Data of new cases of endometrial cancer between 1998 and 2012 were obtained from the Murcia and Bas-Rhin cancer registries. In that period, 3756 cases of endometrial cancer were recorded, with 3270 corresponding to type I EC and 486 corresponding to type II EC. The Bas-Rhin area presented higher age-adjusted incidence rates than those in the Region of Murcia for both type I EC (24.2 and 19.3 cases/100,000 person-years (py), respectively) and type II EC (4.4 and 2.3 cases/100,000 py, respectively). Joinpoint regression showed no changes in trends. In both populations, there was an increasing trend for both EC types, but the trend was steeper in the Region of Murcia and larger overall for type II EC. Finally, a significant increase was observed in the annual trend of type II EC. Further studies are warranted to determine the potential risk factors, and continued efforts are needed to improve the recording and monitoring of EC types.
A Simple Field Tapping Test for Evaluating Frequency Qualities of the Lower Limb Neuromuscular System in Soccer Players: A Validity and Reliability Study
Authors: Chaabouni Safouen , Methnani Rihab , Al Hadabi Badria , Al Busafi Majid , Al Kitani Mahfoodha , Al Jadidi Khalifa , Samozino Pierre , Moalla Wassim , Gmada Nabil
Published: March 2022

Over the years, the foot tapping test protocol has been proposed by scientists to identify the capabilities of the lower limb neuromuscular system in the medical context; however, to our knowledge, no studies have established its usefulness and relationship to athletic performance. The aim of the present study was to test the reliability, criterion validity and sensitivity of a new foot tapping (TAP) test, and to examine its relationship with proxies of athletic performance in soccer players. Forty voluntary soccer players of two different levels participated in this study (20 players from the national level: age: 22.6 ± 2.5 years and 20 players from regional level: 25.1 ± 3.6 years). They performed the TAP test on two separate occasions to test its relative and absolute reliability. To examine the criterion validity of the TAP test, all participants performed four types of jumps, sprint tests, agility tests, the Wingate test and the finger tapping test considered a gold standard tapping test. The sensitivity was assessed with national and regional player levels. The TAP test presented a high relative and absolute reliability with intra-class correlation coefficient ICC > 0.90, standard errors of measurement SEM < 5% and mean difference ±95% limits of agreement equal to 0.2 ± 0.8 tap·s−1. National level players showed a higher TAP score (p < 0.001; dz = 1.96, large) compared to regional players (9.68 ± 1.41 tap·s−1 vs. 7.28 ± 1.01 tap·s−1, respectively) and the value of area under curve measured by the receiver operating characteristic curve technique was 0.95 (95% CI: 0.827–0.990). The TAP test showed a significant association with the finger tapping test (r = 0.84, p < 0.001), whereas no correlation was seen between the TAP test and all the other physical tests measured. The TAP test could be considered a valid and reliable test to assess lower limb neuromuscular ability in soccer players.
Organic Farming as an Alternative Maintenance Strategy in the Opinion of Farmers from Natura 2000 Areas
Authors: Pawlewicz Adam , Gotkiewicz Wojciech , Brodzińska Katarzyna , Pawlewicz Katarzyna , Mickiewicz Bartosz , Kluczek Paweł
Published: March 2022

Agricultural land accounts for approximately 40% of the total Natura 2000 (N2K) network area. Therefore, many habitats and species protected under the Habitats and Birds Directives are dependent on or linked to agricultural practices. This implies that sustaining agriculture of a high natural value is a priority in achieving the aim of halting the loss of biodiversity in the European Union (EU). However, extensive agriculture is unprofitable in many regions of the EU, which results in it being either abandoned or intensified in the absence of financial support. Hence, organic farming (OF), which is most often supported with public funds, can be an alternative to conventional agriculture in N2K areas. This article is an empirical study of the differences in perceiving the possibilities of farm functioning in a protected area (PA) by organic and conventional farm owners. It was examined whether this could be the actual path to improving farmers’ living conditions in the context of legal protection of naturally valuable areas. The study material comprises the results of a survey conducted at the turn of 2016 and 2017, which addressed a total of 292 farmers, including 152 organic farm operators and 140 conventional farm operators, whose areas under cultivation were located within the N2K “Dolina Biebrzy” (“Biebrza Valley”) PLH200008 area in Poland. For the analysis of the data collected using structured questionnaires, a variety of statistical methods and techniques were applied. The study results indicated that in terms of satisfaction with the economic performance of their farms, there is no major difference between the opinions expressed by organic and conventional farm owners. However, organic farming could be an alternative livelihood strategy from the environmental policy perspective.
Impact of COVID-19 Pandemic on Children and Adolescents with Neuropsychiatric Disorders: Emotional/Behavioral Symptoms and Parental Stress
Authors: Operto Francesca Felicia , Coppola Giangennaro , Vivenzio Valentina , Scuoppo Chiara , Padovano Chiara , de Simone Valeria , Rinaldi Rosetta , Belfiore Gilda , Sica Gianpiero , Morcaldi Lucia , D’Onofrio Floriana , Olivieri Miriam , Donadio Serena , Roccella Michele , Carotenuto Marco , Viggiano Andrea , Pastorino Grazia Maria Giovanna
Published: March 2022

The objective of our study was to evaluate the impact of the COVID-19 pandemic on the emotional and behavioral symptoms in minors with neuropsychiatric disorders and on parental stress through a standardized neuropsychological assessment, comparing the data collected before the pandemic with those collected during the lock-down. Another goal of our study was to analyze the relationship between parental stress and behavioral/emotional symptoms in children. Our study was conducted on 383 families of patients who had already been referred at the Child Neuropsychiatry Unit of the University Hospital of Salerno for different neuropsychiatric conditions. All the parents completed two neuropsychological standardized questionnaires for the assessment of parental stress (PSI—Parenting Stress Index-Short Form) and the emotional/behavioral problems of their children (Child Behaviour CheckList). The data collected during the pandemic were compared with those collected from questionnaires administered during the six months preceding the pandemic, as is our usual clinical practice. The comparison between the mean scores of PSI and CBCL before and after the pandemic showed a statistically significant increase in all subscales analyzed in the total sample. The correlation analysis showed significant positive relationship between the subscale Total Stress of PSI and the subscales Total Problems and Internalizing Problems of CBCL. Our study suggested that the COVID-19 pandemic and the corresponding measures adopted led to an increase in internalizing and externalizing symptoms in children and adolescents with neuropsychiatric disorder. Similarly, parental stress increased during COVID-19 and ahigher level of stress in parents can be related to the internalizing symptoms of their children.
Impact of Sleep Deprivation on Job Performance of Working Mothers: Mediating Effect of Workplace Deviance
Authors: Deng Yuwei , Cherian Jacob , Kumari Kalpina , Samad Sarminah , Abbas Jawad , Sial Muhammad Safdar , Popp József , Oláh Judit
Published: March 2022

The current study takes its philosophical roots from organizational behavior and psychology domains to investigate the impact of sleep deprivation on the job performance of mothers working in primary, secondary, and higher education institutions (HEIs) of Pakistan. It also examines the mediating role of workplace deviance in the relationship between sleep deprivation and the job performance of working mothers. The authors followed the non-probability convenience sampling technique to study the relationship between sleep deprivation, workplace deviance, and job performance. The structural analyses indicated that sleep deprivation has a significant negative impact on the job performance of working mothers and sleep-deprived individuals often tend to perform poorly at the workplace. Such workers are also more likely to engage in workplace deviant behaviors. Moreover, workplace deviance is also found to act as a mediating variable in the relationship between sleep deprivation and job performance. The present research bridges the literature gap on the rarely investigated factors, namely sleep deprivation and workplace deviance, and provide a detailed understanding of how these factors can influence the performance of working mothers, specifically in Pakistan.
Respiratory Physiotherapy Intervention Strategies in the Sequelae of Breast Cancer Treatment: A Systematic Review
Authors: Vinolo-Gil Maria , Martín-Valero Rocío , Martín-Vega Francisco , Rodríguez-Huguet Manuel , Perez-Cabezas Veronica , Gonzalez-Medina Gloria
Published: March 2022

Breast cancer treatments can trigger respiratory sequelae. Respiratory physiotherapy helps to eliminate or mitigate the sequelae by optimizing respiratory function. This systematic review aims to synthesize the scientific evidence and assess its quality regarding the use of respiratory physiotherapy in the sequelae of breast cancer. The Cochrane Library, Physiotherapy Evidence Database, PubMed, Web of Science, Scientific Electronic Library Online, Cumulative Index of Nursing and Allied Literature Complete, and Scopus were searched. Study quality was determined using the PEDro scale, STROBE Statement, and Single-Case Experimental Design Scale. Ten studies, six clinical trials, one case study, and three observational studies were selected. The mean methodological quality of the clinical trials was 5.6, that of the case study was 7, and that of the observational studies was 56%. Respiratory physiotherapy has been observed to improve respiratory capacity, lung function, respiratory muscle strength, effort tolerance, dyspnea, fatigue, thoracic mobility, upper limb volume, sleep quality and quality of life, as well as sensitivity to adverse physiological reactions, nausea, vomiting, and anxiety. However, it is not effective for vasomotor symptoms. More clinical trials are needed. These studies should homogenize the techniques used, as well as improve their methodological quality.
Association between Drinking Patterns and Incident Hypertension in Southwest China
Authors: Wang Yawen , Yao Yuntong , Chen Yun , Zhou Jie , Wu Yanli , Fu Chaowei , Wang Na , Liu Tao , Xu Kelin
Published: March 2022

Based on a prospective cohort study of adults from southwest China with heterogeneity in their demographical characteristics and lifestyles, we aimed to explore the association between drinking patterns and incident hypertension under the interaction of these confounding factors. The Cox proportional hazard model was used to estimate the hazard ratios (HR) and 95% confidence intervals (95% CI). Subgroup analysis was performed according to sex, ethnicity, area, occupation, smoking, and exercise to compare the differences in the association between drinking patterns and the incidence of hypertension. Blood pressure was higher in participants with a high drinking frequency than those with a low drinking frequency (p < 0.001). We found that total drinking frequency, liquor drinking frequency, rice wine drinking frequency, and alcohol consumption were significantly associated with an increased risk of hypertension. Compared with the non-drinking group, a heavy drinking pattern was positively correlated with hypertension. Drinking can increase the risk of hypertension, especially heavy drinking patterns, with a high frequency of alcohol intake and high alcohol consumption. From the analysis results of the longitudinal data, drinking alcohol is still an important risk factor for hypertension among Chinese subjects, especially for men, the rural population, the employed, the Han nationality, smokers, and certain exercise populations.
Exposure to War Prior to Conception: Maternal Emotional Distress Forecasts Sex-Specific Child Behavior Problems
Authors: Beijers Roseriet , Scher Anat , Ohana Hanit , Maayan-Metzger Ayala , Leshem Micah
Published: March 2022

Objectives: Exposure to maternal stress during the prenatal period adversely affects child outcomes. Recent investigations have shifted to an even earlier period, the preconception period, to better understand the role of this formative period in human health and disease. We investigated the links between maternal emotional distress following preconception exposure to war, and child outcomes at age 10. Material and Methods: Before becoming pregnant, mothers were exposed to missile bombardment on the north of Israel in the 2006 war. Mothers who conceived within 12 months after the war were recruited and compared to mothers who conceived during the same period but lived in Israel but outside missile range. During the initial assessment, mothers completed a questionnaire on emotional distress. At 10 years of age, mothers and children (N = 68) reported on child socio-emotional outcomes. Results: Multiple regression analyses revealed that, in girls, higher maternal emotional distress following preconception war exposure predicted more internalizing and externalizing behavior problems, and more behavior regulation problems. In boys, maternal emotional distress was not significantly related to outcomes. Conclusion: Maternal emotional distress following preconception exposure to war forecasts sex-specific child behavioral problems as reported by the mother and the child. Though the results warrant cautious interpretation because of the relatively small sample size and differential attrition, our findings add to the small but growing body of research on the consequences of maternal stress exposure prior to conception for the next generation.
The Effect of Deep Micro Vibrotactile Stimulation on Cognitive Function of Mild Cognitive Impairment and Mild Dementia
Authors: Kodama Ayuto , Suzuki Yasuhiro , Sakuraba Kazuki , Kume Yu , Ota Hidetaka
Published: March 2022

Background: The purpose of this study was to clarify the effect of Deep Micro Vibrotactile (DMV) stimulation on the cognitive functions in elderly people with mild cognitive impairment or mild dementia. Methods: A total of 35 participants with dementia from three nursing homes, who had completed treatment with DMV stimulation at 15–40 Hz (hereinafter, 15–40 Hz DMV stimulation) for a month were recruited for this study. The subjects had received continuous 15–40 Hz DMV stimulation for 24 h a day for 1 month. We assessed the effect of the treatment on the cognitive functions (by the word list memory (WM) test, trail making test-part A (TMT-A) and part B (TMT-B), and symbol digit substitution task (SDST)) and physical functions (grip strength (GS) and usual walking speed (UWS)), by comparing the results at the baseline and after the 1-month intervention (DMV stimulation). Results: The results revealed that the performances in the WM test (p < 0.05), TMT-B (p < 0.05), and SDST (p < 0.01) improved significantly after the intervention. Conclusion: Our findings suggest that 15–40 Hz DMV stimulation is might be effective for improving the cognitive functions in elderly people with dementia. Furthermore, our novel findings showed the different effectiveness of the treatment depending on the stage of cognitive impairments.
Does the Adoption of Climate-Smart Agricultural Practices Impact Farmers’ Income? Evidence from Ghana
Authors: Agbenyo Wonder , Jiang Yuansheng , Jia Xinxin , Wang Jingyi , Ntim-Amo Gideon , Dunya Rahman , Siaw Anthony , Asare Isaac , Twumasi Martinson Ankrah
Published: March 2022

People’s lives, particularly farmers’, have been affected by extreme weather conditions that have reduced the yield of numerous crops due to climate change. Climate-smart agriculture practices can reduce or eliminate greenhouse gas emissions and have the propensity to increase farm income and productivity. Therefore, the purpose of this study is to ascertain whether CSA practices impact farmers’ income. This study includes all cocoa farmers in the selected districts in the Ashanti Region. The population includes those who live in the six cocoa production villages. The multistage sampling procedure was considered based on the dominants of literature. The study used an endogenous switching regression framework to examine the effects of the adoption of climate-smart agricultural practices (CSAPs) on farmers’ income. While estimating treatment effects, telasso uses lasso techniques to select the appropriate variable sets. The results revealed that gender, farm experience, age, household size, and farm size do not significantly influence the adoption of irrigation and crop insurance. The study revealed a significant positive impact of access to credit on adopting irrigation and crop insurance. The adoption of climate-smart practices has a positive coefficient. This indicates that if all respondents in each region adopts these practices, their income would increase significantly. This study shows that adopting irrigation practices leads to an increase in household income of 8.6% and 11.1%, respectively, for cocoa farmers. Crop insurance has a positive coefficient and is statistically significant on household income, on-farm, and off-farm. This paper shows that climate-smart practices such as crop insurance can positively influence farmers’ income in Ghana. We also conjecture that crop insurance is the most effective and efficient climate-smart practice among the various agricultural practices. The study suggests that access to credit and mass awareness should be compulsory modules coupled with the consistent training of farmers on new technologies for effective policy implementation. Expanding access to extension officers could enhance farmers’ adaptive capacity and warrant the efficiency of implemented practices.
Suggestions for Improvements in National Radon Control Strategies of Member States Which Were Developed as a Requirement of EU Directive 2013/59 EURATOM
Authors: Mc Laughlin James P. , Gutierrez-Villanueva Jose-Luis , Perko Tanja
Published: March 2022

Exposure to the indoor air pollutant radon is considered to be a significant health risk globally, as has been demonstrated by many studies over time. A recent WHO statement on radon estimates that, worldwide, approximately 80,000 people may die every year due to lung cancer associated with radon exposure. The recent years have also seen huge improvements in radon policies in European countries, as a consequence of the issuing, in 2013, of the Council Directive 2013/59/Euratom. Although the protection of workers from radon exposure is well established, the protection of the general public needs more improvements. The main objective of this paper is, first, to acknowledge and recognise the improvements in radon protection policies, but also to show that there are many areas where improvements are desirable and possible. The final goal is to suggest better ways to protect the general population from exposure to radon gas. The suggestions are based on the experiences of the co-authors, who come from different disciplines related to radon management. The following fields or areas where improvements are possible are identified: risk communication, building codes, radon policies, including funding, research and protection of children. We describe the work that has been conducted, and the possible improvements and solutions in these fields.
The Impact of Four High-Altitude Training Camps on the Aerobic Capacity of a Short Track PyeongChang 2018 Olympian: A Case Study
Authors: Lukanova-Jakubowska Anna , Piechota Katarzyna , Grzywacz Tomasz , Ambroży Tadeusz , Rydzik Łukasz , Ozimek Mariusz
Published: March 2022

This study characterizes high-altitude training camps and their effect on the aerobic capacity of a Polish national team member (M.W.), who was a participant in the PyeongChang 2018 Winter Olympic Games (body weight: 59.6 kg, body height: 161.0 cm, fat mass: 10.9 kg and 18.3% of fat tissue, fat-free mass: 48.7 kg, muscle mass: 46.3 kg, and BMI = 23.0 kg/m2). The tests were conducted in the periods from April 2018 to September 2018 and April 2019 to September 2019 (period of general and special preparation). The study evaluated aerobic and anaerobic capacity determined by laboratory tests, a cardiopulmonary graded exercise test to exhaustion performed on a cycle ergometer (CPET), and the Wingate anaerobic test. Based on the research, training in hypobaric conditions translated into significant improvements in the skater’s exercise capacity recorded after participating in the Olympic Winter Games in Korea (February 2018). In the analyzed period (2018–2019), there was a significant increase in key parameters of aerobic fitness such as anaerobic threshold power output (AT-PO) [W]—223; power output POmax [W]—299 and AT-PO [W/kg]—3.50; (POmax) [W/kg]—4.69; and AT-VO2 [mL/kg/min]—51.3; VO2max [mL/kg/min]—61.0. The athlete showed high-exercise-induced adaptations and improvements in the aerobic metabolic potential after two seasons, in which four training camps were held in altitude conditions.
FLOWER: An Approach for Enhancing E-Learning Experience Amid COVID-19
Authors: Elshaer Ibrahim A. , Sobaih Abu Elnasr E.
Published: March 2022

The worldwide COVID-19 pandemic has forced higher institutions to shift towards electronic (e) learning. Despite a plethora of research on the responses of higher education institutions to COVID-19 and their shift towards e-learning, research often focuses on the opportunities and/or challenges of e-learning amid COVID-19. Notwithstanding this, limited research has addressed how e-learning experiences can be enhanced among medical students, who often need conventional leaning, especially for practical courses. This research addresses a gap in the knowledge and examined medical students’ perceptions of e-learning using the Blackboard platform, and the elements or predicators that affect their e-learning experience amid COVID-19 in the Kingdom of Saudi Arabia. An online survey was transmitted to medical students in three main public universities. Based on the findings, a FLOWER model was proposed for improving e-learning experience using Blackboard among medical students. This model includes six dimensions: feedback, leverage to remain motivated, open resources and information, working together, evaluation, and reflection and knowledge. These dimensions are interrelated, and enable the creation of a positive e-learning experience. The results showed that four of the six dimensions have high positive and significant path coefficients: open sources and information; leverage to remain motivated; working together; and reflection and knowledge construction. Two of the six dimensions have low positive, but significant, path coefficients (feedback and evaluation), which require further consideration by policymakers and educators. The results have several theoretical and practical implications, which are elaborated upon.
State-Level Prevalence and Associates of Opioid Dependence in the USA
Authors: Leung Janni , Chan Gary C. K. , Tan Samuel X. , McClure-Thomas Caitlin , Degenhardt Louisa , Hall Wayne
Published: March 2022

Traditionally, opioid-related disease burden was primarily due to heroin use. However, increases in extra-medical (or non-medicinal use of prescription opioids; NMPOs) use has precipitated the current overdose epidemic in North America. We aim to examine the state-level prevalence of heroin and NMPO dependence and their associations with opioid-related mortality and state-level socio-demographic profiles. Data were pooled from the 2005–2014 National Survey on Drug Use and Health (NSDUH). We examine opioid-related mortality from CDC WONDER (Cause of Death database) by the past year prevalence of DSM-IV heroin and NMPO dependence, by age and sex, and their associations with state-level socio-demographic characteristics from census data. State-level rates of heroin dependence were associated with opioid-related death rates in young and mid-aged adults, while rates of NMPO dependence were associated with opioid-related death rates across all ages. The prevalence of heroin dependence was positively associated with state-level GDP/capita and urbanity. State-level NMPO dependence prevalence was associated with higher unemployment, lower GDP/capita, and a lower high-school completion rate. The prevalence of heroin and NMPO dependence are associated with a broad range of geographical and socio-demographic groups. Taking a wider view of populations affected by the opioid epidemic, inclusive interventions for all are needed to reduce opioid-related disease burden.
Breakdown of Diabetic Foot Ulcer Care during the First Year of the Pandemic in Poland: A Retrospective National Cohort Study
Authors: Kleibert Marcin , Mrozikiewicz-Rakowska Beata , Bąk Patrycja Małgorzata , Bałut Daniel , Zieliński Jakub , Czupryniak Leszek
Published: March 2022

The COVID-19 pandemic revealed a breakdown of the system of DFU patient care. This retrospective national cohort study analyses the epidemiological status of DFU patients in relation to urgent and elective hospitalizations, amputation rates, and deaths in Poland from 2017 to 2019, and during 2020 when the COVID-19 pandemic began. The data were obtained from national medical records gathered by the National Health Fund (NHF). Discharge diagnoses were categorized according to ICD-10 and ICD-9 codes. Analysis of the data showed a statistically significant decrease in elective hospital admissions (from 29.6% to 26.3%, p = 0.001). There was a decrease in the percentage of hospitalizations related to limb-salvage procedures (from 79.4% to 71.3%, p = 0.001). The opposite tendency was observed among urgent hospital admissions (from 67.0% to 73.2%, p = 0.01), which was related to a significant increase in the number of minor amputations (from 3146 to 4269, p = 0.017). This rise was in parallel with the increase in the percentage of patients who died during hospitalization due to DFU (from 3.9% to 4.8%, p = 0.03). The number of deaths has not changed significantly (from 590.7 to 668.0, p = 0.26). The results of the conducted analyses confirm the negative tendencies in the medical care of patients with DFU during the first year of the pandemic in Poland. Changes in therapy schemes and stronger patient support following this period are necessary to avoid further complications in patients with DFU.
A Comparative Multi-System Approach to Characterizing Bioactivity of Commonly Occurring Chemicals
Authors: Rivera Brianna N. , Wilson Lindsay B. , Kim Doo Nam , Pande Paritosh , Anderson Kim A. , Tilton Susan C. , Tanguay Robyn L.
Published: March 2022

A 2019 retrospective study analyzed wristband personal samplers from fourteen different communities across three different continents for over 1530 organic chemicals. Investigators identified fourteen chemicals (G14) detected in over 50% of personal samplers. The G14 represent a group of chemicals that individuals are commonly exposed to, and are mainly associated with consumer products including plasticizers, fragrances, flame retardants, and pesticides. The high frequency of exposure to these chemicals raises questions of their potential adverse human health effects. Additionally, the possibility of exposure to mixtures of these chemicals is likely due to their co-occurrence; thus, the potential for mixtures to induce differential bioactivity warrants further investigation. This study describes a novel approach to broadly evaluate the hazards of personal chemical exposures by coupling data from personal sampling devices with high-throughput bioactivity screenings using in vitro and non-mammalian in vivo models. To account for species and sensitivity differences, screening was conducted using primary normal human bronchial epithelial (NHBE) cells and early life-stage zebrafish. Mixtures of the G14 and most potent G14 chemicals were created to assess potential mixture effects. Chemical bioactivity was dependent on the model system, with five and eleven chemicals deemed bioactive in NHBE and zebrafish, respectively, supporting the use of a multi-system approach for bioactivity testing and highlighting sensitivity differences between the models. In both NHBE and zebrafish, mixture effects were observed when screening mixtures of the most potent chemicals. Observations of BMC-based mixtures in NHBE (NHBE BMC Mix) and zebrafish (ZF BMC Mix) suggested antagonistic effects. In this study, consumer product-related chemicals were prioritized for bioactivity screening using personal exposure data. High-throughput high-content screening was utilized to assess the chemical bioactivity and mixture effects of the most potent chemicals.
Prospects for the Development of the Demand for Carp in Poland among Young Consumers
Authors: Raftowicz Magdalena
Published: March 2022

Carp fishing economy in Poland has a centuries-old tradition. However, in the last decade, as a result of changes in global market trends, this industry has experienced stagnation. Still, the elimination of this niche industry may have painful consequences for the entire ecosystem and biodiversity. Hence, every effort should be made to protect and maintain the status quo. The aim of the article is an attempt to show that the development prospects for the carp market in Poland are limited, especially in the face of little interest in carp consumption by young adult consumers, who will create the demand for carp in the near future. The remedy may be to change the image of the carp together with a territorial marketing strategy that would be consistent with the preferences of the young generation. The research was conducted on the basis of a critical analysis of the literature of the subject, focus studies, questionnaires and a case study.
Telework and Face-to-Face Work during COVID-19 Confinement: The Predictive Factors of Work-Related Stress from a Holistic Point of View
Authors: Soubelet-Fagoaga Iduzki , Arnoso-Martinez Maitane , Elgorriaga-Astondoa Edurne , Martínez-Moreno Edurne
Published: March 2022

This article explores the socio-labor conditions in which people worked during confinement, analyzing the predictors of work-related stress, according to work modality (face-to-face or teleworking), from a holistic and quantitative (n = 328) point of view. To identify predictors of stress, correlational analyses and multiple hierarchical regressions were conducted with individual, organizational, and societal variables. Furthermore, to analyze the possible modulating role of gender, caregiving, and the level of responsibility in organizations in the relationship between predictor variables and work stress, the macro process of Hayes was used. Our results show that work–family conflict and ruminative thoughts predict stress in both modalities. In teleworking modality, the hours dedicated to work predicted stress, and in face-to-face modality, safety measures and perceived economic threat (tendentially). Being in charge of persons moderated the relationship between ruminative thoughts and economic threat, and stress in face-to-face. Results are discussed by identifying good practices that can improve workplace risk prevention strategies.
A Generalized 9-Intersection Model for Topological Relations between Regions with Holes
Authors: Leng Liang , Wang Fengyan , Wang Mingchang , Yang Guodong , Niu Xuefeng , Zhang Xuqing
Published: March 2022

Current models cannot distinguish detailed topological relations between regions with holes. In order to solve this problem, a new detailed representation model for topological relations is proposed in this study. In this model, the key is to describe topological relations caused by multi-holes. Definitions of regions with holes and real objects expressed by elements of regions with holes are comprehensively analyzed, and then a practical definition of regions with holes is presented. Based on the 9-intersection model, a generalized 9-intersection model is proposed, which can completely describe detailed topological relations between regions with holes. For showing the description ability, the model is applied to describe detailed topological relations between regions with holes and detailed topological relations between objects of different complexities with the same major categories of topological relations, and a case study is designed to show the practicality of the model. The results show that the model is valid.
Daily Human Mobility: A Reproduction Model and Insights from the Energy Concept
Authors: Wang Weiying , Osaragi Toshihiro
Published: March 2022

Human movements have raised broad attention, and many models have been developed to reproduce them. However, most studies focus on reproducing the statistical properties of human mobility, such as the travel distance and the visiting frequency. In this paper, a two-step Markov Chain model is proposed to generate daily human movements, and spatial and spatiotemporal attributes of reproduced mobility are examined. In the first step, people’s statuses in the next time slot are conditioned on their previous travel patterns; and in the second step, individual location in such a slot is probabilistically determined based on his/her status. Our model successfully reproduces the spatial and spatiotemporal characteristics of human daily movements, and the result indicates that people’s future statuses can be inferred based on travel patterns they made, regardless of exactly where they have traveled, and when trips happen. We also revisit the energy concept, and show that the energy expenditure is stable over years. This idea is further used to predict the proportion of long-distance trips for each year, which gives insights into the probabilities of statuses in the next time slot. Finally, we interpret the constant energy expenditure as the constant ‘cost’ over years.
Therapeutic Applications of Adeno-Associated Virus (AAV) Gene Transfer of HLA-G in the Eye
Authors: Gilger Brian C. , Hirsch Matthew L.
Published: March 2022

The purpose of this paper is to review human leukocyte antigen G (HLA-G) in the eye, its role in immune tolerance, and the potential therapeutic use of AAV gene transfer and expression of HLA-G in various ocular tissues. Several studies are reviewed that demonstrate efficacy in animal models of disease, including intracorneal delivery of AAV-HLA-G to treat corneal inflammation and prevent corneal graft rejection, subconjunctival injection of AAV-HLA-G for ocular graft vs. host disease and potentially dry eye disease, and intravitreal injection of AAV-HLA-G to inhibit uveitis. Furthermore, due to the anti-vascular function of HLA-G, AAV-HLA-G may be an effective therapy for posterior ocular diseases, such as neovascular age-related macular degeneration, diabetic retinopathy, and choroidal neovascularization. Therefore, AAV-mediated gene transfer of HLA-G may be an effective treatment for common immune-mediated, inflammatory, and neovascular diseases of the eye.
Structural and Biophysical Insights into SPINK1 Bound to Human Cationic Trypsin
Authors: Nagel Felix , Palm Gottfried J. , Geist Norman , McDonnell Thomas C. R. , Susemihl Anne , Girbardt Britta , Mayerle Julia , Lerch Markus M. , Lammers Michael , Delcea Mihaela
Published: March 2022

(1) The serine protease inhibitor Kazal type 1 (SPINK1) inhibits trypsin activity in zymogen granules of pancreatic acinar cells. Several mutations in the SPINK1 gene are associated with acute recurrent pancreatitis (ARP) and chronic pancreatitis (CP). The most common variant is SPINK1 p.N34S. Although this mutation was identified two decades ago, the mechanism of action has remained elusive. (2) SPINK1 and human cationic trypsin (TRY1) were expressed in E. coli, and inhibitory activities were determined. Crystals of SPINK1–TRY1 complexes were grown by using the hanging-drop method, and phases were solved by molecular replacement. (3) Both SPINK1 variants show similar inhibitory behavior toward TRY1. The crystal structures are almost identical, with minor differences in the mutated loop. Both complexes show an unexpected rotamer conformation of the His63 residue in TRY1, which is a member of the catalytic triad. (4) The SPINK1 p.N34S mutation does not affect the inhibitory behavior or the overall structure of the protein. Therefore, the pathophysiological mechanism of action of the p.N34S variant cannot be explained mechanistically or structurally at the protein level. The observed histidine conformation is part of a mechanism for SPINK1 that can explain the exceptional proteolytic stability of this inhibitor.
MicroRNAs in Leukemias: A Clinically Annotated Compendium
Authors: Turk Aleksander , Calin George A. , Kunej Tanja
Published: March 2022

Leukemias are a group of malignancies of the blood and bone marrow. Multiple types of leukemia are known, however reliable treatments have not been developed for most leukemia types. Furthermore, even relatively reliable treatments can result in relapses. MicroRNAs (miRNAs) are a class of short, noncoding RNAs responsible for epigenetic regulation of gene expression and have been proposed as a source of potential novel therapeutic targets for leukemias. In order to identify central miRNAs for leukemia, we conducted data synthesis using two databases: miRTarBase and DISNOR. A total of 137 unique miRNAs associated with 16 types of leukemia were retrieved from miRTarBase and 86 protein-coding genes associated with leukemia were retrieved from the DISNOR database. Based on these data, we formed a visual network of 248 miRNA-target interactions (MTI) between leukemia-associated genes and miRNAs associated with ≥4 leukemia types. We then manually reviewed the literature describing these 248 MTIs for interactions identified in leukemia studies. This manually curated data was then used to visualize a network of 64 MTIs identified in leukemia patients, cell lines and animal models. We also formed a visual network of miRNA-leukemia associations. Finally, we compiled leukemia clinical trials from the ClinicalTrials database. miRNAs with the highest number of MTIs were miR-125b-5p, miR-155-5p, miR-181a-5p and miR-19a-3p, while target genes with the highest number of MTIs were TP53, BCL2, KIT, ATM, RUNX1 and ABL1. The analysis of 248 MTIs revealed a large, highly interconnected network. Additionally, a large MTI subnetwork was present in the network visualized from manually reviewed data. The interconnectedness of the MTI subnetwork suggests that certain miRNAs represent central disease molecules for multiple leukemia types. Additional studies on miRNAs, their target genes and associated biological pathways are required to elucidate the therapeutic potential of miRNAs in leukemia.
State of the Art in the Current Management and Future Directions of Targeted Therapy for Differentiated Thyroid Cancer
Authors: Silaghi Horatiu , Lozovanu Vera , Georgescu Carmen Emanuela , Pop Cristina , Nasui Bogdana Adriana , Cătoi Adriana Florinela , Silaghi Cristina Alina
Published: March 2022

Two-thirds of differentiated thyroid cancer (DTC) patients with distant metastases would be classified as radioactive iodine-refractory (RAIR-DTC), evolving into a poor outcome. Recent advances underlying DTC molecular mechanisms have shifted the therapy focus from the standard approach to targeting specific genetic dysregulations. Lenvatinib and sorafenib are first-line, multitargeted tyrosine kinase inhibitors (TKIs) approved to treat advanced, progressive RAIR-DTC. However, other anti-angiogenic drugs, including single targeted TKIs, are currently being evaluated as alternative or salvage therapy after the failure of first-line TKIs. Combinatorial therapy of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) signalling cascade inhibitors has become a highly advocated strategy to improve the low efficiency of the single agent treatment. Recent studies pointed out targetable alternative pathways to overcome the resistance to MAPK and PI3K pathways’ inhibitors. Because radioiodine resistance originates in DTC loss of differentiation, redifferentiation therapies are currently being explored for efficacy. The present review will summarize the conventional management of DTC, the first-line and alternative TKIs in RAIR-DTC, and the approaches that seek to overcome the resistance to MAPK and PI3K pathways’ inhibitors. We also aim to emphasize the latest achievements in the research of redifferentiation therapy, immunotherapy, and agents targeting gene rearrangements in advanced DTC.
Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition
Authors: Dussart Francois , Jakubczyk Dorota
Published: March 2022

The important disease Ramularia leaf spot of barley is caused by the fungus Ramularia collo-cygni. The disease causes yield and quality losses as a result of a decrease in photosynthesis efficiency due to the appearance of necrotic spots on the leaf surface. The development of these typical Ramularia leaf spot symptoms is thought to be linked with the release of phytotoxic secondary metabolites called rubellins in the host. However, to date, neither the biosynthetic pathways leading to the production of these metabolites nor their exact role in disease development are known. Using a combined in silico genetic and biochemistry approach, we interrogated the genome of R. collo-cygni to identify a putative rubellin biosynthetic gene cluster. Here we report the identification of a gene cluster containing homologues of genes involved in the biosynthesis of related anthraquinone metabolites in closely related fungi. A putative pathway to rubellin biosynthesis involving the genes located on the candidate cluster is also proposed.
Progress in Bioengineering Strategies for Heart Regenerative Medicine
Authors: Häneke Timm , Sahara Makoto
Published: March 2022

The human heart has the least regenerative capabilities among tissues and organs, and heart disease continues to be a leading cause of mortality in the industrialized world with insufficient therapeutic options and poor prognosis. Therefore, developing new therapeutic strategies for heart regeneration is a major goal in modern cardiac biology and medicine. Recent advances in stem cell biology and biotechnologies such as human pluripotent stem cells (hPSCs) and cardiac tissue engineering hold great promise for opening novel paths to heart regeneration and repair for heart disease, although these areas are still in their infancy. In this review, we summarize and discuss the recent progress in cardiac tissue engineering strategies, highlighting stem cell engineering and cardiomyocyte maturation, development of novel functional biomaterials and biofabrication tools, and their therapeutic applications involving drug discovery, disease modeling, and regenerative medicine for heart disease.
High Risk-Human Papillomavirus in HNSCC: Present and Future Challenges for Epigenetic Therapies
Authors: Ghiani Lavinia , Chiocca Susanna
Published: March 2022

Head and Neck Squamous Cell Carcinoma (HNSCC) is a highly heterogeneous group of tumors characterized by an incidence of 650,000 new cases and 350,000 deaths per year worldwide and a male to female ratio of 3:1. The main risk factors are alcohol and tobacco consumption and Human Papillomavirus (HPV) infections. HNSCC cases are divided into two subgroups, the HPV-negative (HPV−) and the HPV-positive (HPV+) which have different clinicopathological and molecular profiles. However, patients are still treated with the same therapeutic regimens. It is thus of utmost importance to characterize the molecular mechanisms underlying these differences to find new biomarkers and novel therapeutic targets towards personalized therapies. Epigenetic alterations are a hallmark of cancer and can be exploited as both promising biomarkers and potential new targets. E6 and E7 HPV oncoviral proteins besides targeting p53 and pRb, impair the expression and the activity of several epigenetic regulators. While alterations in DNA methylation patterns have been well described in HPV+ and HPV− HNSCC, accurate histone post-translational modifications (hPTMs) characterization is still missing. Herein, we aim to provide an updated overview on the impact of HPV on the hPTMs landscape in HNSCC. Moreover, we will also discuss the sex and gender bias in HNSCC and how the epigenetic machinery could be involved in this process, and the importance of taking into account sex and/or gender also in this field.
The Role of CCL2/CCR2 Axis in Cerebral Ischemia-Reperfusion Injury and Treatment: From Animal Experiments to Clinical Trials
Authors: Geng Huixia , Chen Luna , Tang Jing , Chen Yi’ang , Wang Lai
Published: March 2022

C-C motif chemokine ligand 2 (CCL2) is a member of the monocyte chemokine protein family, which binds to its receptor CCR2 to induce monocyte infiltration and mediate inflammation. The CCL2/CCR2 signaling pathway participates in the transduction of neuroinflammatory information between all types of cells in the central nervous system. Animal studies and clinical trials have shown that CCL2/CCR2 mediate the pathological process of ischemic stroke, and a higher CCL2 level in serum is associated with a higher risk of any form of stroke. In the acute phase of cerebral ischemia-reperfusion, the expression of CCL2/CCR2 is increased in the ischemic penumbra, which promotes neuroinflammation and enhances brain injury. In the later phase, it participates in the migration of neuroblasts to the ischemic area and promotes the recovery of neurological function. CCL2/CCR2 gene knockout or activity inhibition can reduce the nerve inflammation and brain injury induced by cerebral ischemia-reperfusion, suggesting that the development of drugs regulating the activity of the CCL2/CCR2 signaling pathway could be used to prevent and treat the cell injury in the acute phase and promote the recovery of neurological function in the chronic phase in ischemic stroke patients.