bluuebunny/crossref_metadata_2025 huggingface dataset:
Highly Sensitive Electrochemical Determination of Butylated Hydroxyanisole in Food Samples Using Electrochemical-Pretreated Three-Dimensional Graphene Electrode Modified with Silica Nanochannel Film
Authors: Huang Chengqing , Zhang Shiyue , Ma Xinying , Yan Fei , Tang Weizhong
Published: March 2024

The sensitive detection of antioxidants in food is essential for the rational control of their usage and reducing potential health risks. A simple three-dimensional (3D) electrode integrated with an anti-fouling/anti-interference layer possesses great potential for the direct and sensitive electrochemical detection of antioxidants in food samples. In this work, a 3D electrochemical sensor was developed by integrating a 3D graphene electrode (3DG) with vertically ordered mesoporous silica film (VMSF), enabling highly sensitive detection of the common antioxidant, butylated hydroxyanisole (BHA), in food samples. A simple electrochemical polarization was employed to pre-activate the 3DG electrode (p3DG), enhancing its hydrophilicity. Using the p3DG as the supporting electrode, stable modification of VMSF was achieved using the electrochemical assisted self-assembly (EASA) method, without the need for any adhesive agents (VMSF/p3DG). Taking BHA in food as a model analyte, the VMSF/p3DG sensor demonstrated high sensitivity, due to the enrichment by nanochannels, towards BHA. Electrochemical detection of BHA was achieved with a linear range of 0.1 μM to 5 μM and from 5 μM to 150 μM with a low limit of detection (12 nM). Owing to the fouling resistance and anti-interference capabilities of VMSF, the constructed 3D electrochemical sensor can be directly applied for the electrochemical detection of BHA in complex food samples.
Poly(Allylamine Hydrochloride) and ZnO Nanohybrid Coating for the Development of Hydrophobic, Antibacterial, and Biocompatible Textiles
Authors: Matijaković Mlinarić Nives , Wawrzaszek Barbara , Kowalska Klaudia , Selmani Atiđa , Učakar Aleksander , Vidmar Janja , Kušter Monika , Van de Velde Nigel , Trebše Polonca , Sever Škapin Andrijana , Jerman Ivan , Abram Anže , Zore Anamarija , Roblegg Eva , Bohinc Klemen
Published: March 2024

In healthcare facilities, infections caused by Staphylococcus aureus (S. aureus) from textile materials are a cause for concern, and nanomaterials are one of the solutions; however, their impact on safety and biocompatibility with the human body must not be neglected. This study aimed to develop a novel multilayer coating with poly(allylamine hydrochloride) (PAH) and immobilized ZnO nanoparticles (ZnO NPs) to make efficient antibacterial and biocompatible cotton, polyester, and nylon textiles. For this purpose, the coated textiles were characterized with profilometry, contact angles, and electrokinetic analyzer measurements. The ZnO NPs on the textiles were analyzed by scanning electron microscopy and inductively coupled plasma mass spectrometry. The antibacterial tests were conducted with S. aureus and biocompatibility with immortalized human keratinocyte cells. The results demonstrated successful PAH/ZnO coating formation on the textiles, demonstrating weak hydrophobic properties. Furthermore, PAH multilayers caused complete ZnO NP immobilization on the coated textiles. All coated textiles showed strong growth inhibition (2–3-log reduction) in planktonic and adhered S. aureus cells. The bacterial viability was reduced by more than 99%. Cotton, due to its better ZnO NP adherence, demonstrated a slightly higher antibacterial performance than polyester and nylon. The coating procedure enables the binding of ZnO NPs in an amount (<30 µg cm−2) that, after complete dissolution, is significantly below the concentration causing cytotoxicity (10 µg mL−1).
Hybrid-Capture Target Enrichment in Human Pathogens: Identification, Evolution, Biosurveillance, and Genomic Epidemiology
Authors: Quek Z. B. Randolph , Ng Sock Hoon
Published: March 2024

High-throughput sequencing (HTS) has revolutionised the field of pathogen genomics, enabling the direct recovery of pathogen genomes from clinical and environmental samples. However, pathogen nucleic acids are often overwhelmed by those of the host, requiring deep metagenomic sequencing to recover sufficient sequences for downstream analyses (e.g., identification and genome characterisation). To circumvent this, hybrid-capture target enrichment (HC) is able to enrich pathogen nucleic acids across multiple scales of divergences and taxa, depending on the panel used. In this review, we outline the applications of HC in human pathogens—bacteria, fungi, parasites and viruses—including identification, genomic epidemiology, antimicrobial resistance genotyping, and evolution. Importantly, we explored the applicability of HC to clinical metagenomics, which ultimately requires more work before it is a reliable and accurate tool for clinical diagnosis. Relatedly, the utility of HC was exemplified by COVID-19, which was used as a case study to illustrate the maturity of HC for recovering pathogen sequences. As we unravel the origins of COVID-19, zoonoses remain more relevant than ever. Therefore, the role of HC in biosurveillance studies is also highlighted in this review, which is critical in preparing us for the next pandemic. We also found that while HC is a popular tool to study viruses, it remains underutilised in parasites and fungi and, to a lesser extent, bacteria. Finally, weevaluated the future of HC with respect to bait design in the eukaryotic groups and the prospect of combining HC with long-read HTS.
In Vivo Anti-Inflammatory and Antinociceptive Activities of Black Elder (Sambucus nigra L.) Fruit and Flower Extracts
Authors: Seymenska Daniela , Teneva Desislava , Nikolova Irina , Benbassat Niko , Denev Petko
Published: March 2024

Sambucus nigra L. (S. nigra, SN) or black elder is a traditional medicinal plant widely used worldwide for therapeutic and dietary purposes. The aim of the current study was to investigate the anti-inflammatory and antinociceptive activities of black elder fruit and flower extracts (SNFrE and SNFlE, respectively). The primary polyphenol constituents in the flower extract were flavonoids and phenolic acids, while anthocyanins were the main components in the fruit extract. SNFrE revealed pronounced and dose-dependent in vivo anti-inflammatory activity assessed by the cotton pellet-induced granuloma test. Doses of 10, 20, and 50 mg/kg BW of SNFrE reduced the weight of induced granuloma in rats by 20.3%, 20.5%, and 28.4%, respectively. At the highest dose (50 mg/kg BW), SNFrE had significant (p < 0.01) anti-inflammatory activity comparable to that of diclofenac, the reference compound used (10 mg/kg BW). In addition, the in vivo antinociceptive activity of the extracts in mice was estimated using the acetic-acid-induced writhing test. Both extracts at doses of 50 mg/kg BW inhibited the abdominal contractions induced by the acetic acid significantly comparing to the control group (p < 0.01). Our findings indicate that black elder extracts and particularly SNFrE possess anti-inflammatory and antinociceptive activities, providing experimental evidence for the use of S. nigra in traditional medicine.
Intranasal Administration of Mesenchymal Stem Cell-Derived Exosome Alleviates Hypoxic-Ischemic Brain Injury
Authors: Ikeda Takuma , Kawabori Masahito , Zheng Yuyuan , Yamaguchi Sho , Gotoh Shuho , Nakahara Yo , Yoshie Erika , Fujimura Miki
Published: March 2024

Hypoxic-ischemic brain injury arises from inadequate oxygen delivery to the brain, commonly occurring following cardiac arrest, which lacks effective treatments. Recent studies have demonstrated the therapeutic potential of exosomes released from mesenchymal stem cells. Given the challenge of systemic dilution associated with intravenous administration, intranasal delivery has emerged as a promising approach. In this study, we investigate the effects of intranasally administered exosomes in an animal model. Exosomes were isolated from the cell supernatants using the ultracentrifugation method. Brain injury was induced in Sprague-Dawley rats through a transient four-vessel occlusion model. Intranasal administration was conducted with 3 × 108 exosome particles in 20 µL of PBS or PBS alone, administered daily for 7 days post-injury. Long-term cognitive behavioral assessments, biodistribution of exosomes, and histological evaluations of apoptosis and neuroinflammation were conducted. Exosomes were primarily detected in the olfactory bulb one hour after intranasal administration, subsequently distributing to the striatum and midbrain. Rats treated with exosomes exhibited substantial improvement in cognitive function up to 28 days after the insult, and demonstrated significantly fewer apoptotic cells along with higher neuronal cell survival in the hippocampus. Exosomes were found to be taken up by microglia, leading to a decrease in the expression of cytotoxic inflammatory markers.
Impact of Drug-Mediated Inhibition of Intestinal Transporters on Nutrient and Endogenous Substrate Disposition…an Afterthought?
Authors: Kharve Kshitee , Engley Andrew S. , Paine Mary F. , Sprowl Jason A.
Published: March 2024

A large percentage (~60%) of prescription drugs and new molecular entities are designed for oral delivery, which requires passage through a semi-impervious membrane bilayer in the gastrointestinal wall. Passage through this bilayer can be dependent on membrane transporters that regulate the absorption of nutrients or endogenous substrates. Several investigations have provided links between nutrient, endogenous substrate, or drug absorption and the activity of certain membrane transporters. This knowledge has been key in the development of new therapeutics that can alleviate various symptoms of select diseases, such as cholestasis and diabetes. Despite this progress, recent studies revealed potential clinical dangers of unintended altered nutrient or endogenous substrate disposition due to the drug-mediated disruption of intestinal transport activity. This review outlines reports of glucose, folate, thiamine, lactate, and bile acid (re)absorption changes and consequent adverse events as examples. Finally, the need to comprehensively expand research on intestinal transporter-mediated drug interactions to avoid the unwanted disruption of homeostasis and diminish therapeutic adverse events is highlighted.
Immune Specific and Tumor-Dependent mRNA Vaccines for Cancer Immunotherapy: Reprogramming Clinical Translation into Tumor Editing Therapy
Authors: Katopodi Theodora , Petanidis Savvas , Grigoriadou Eirini , Anestakis Doxakis , Charalampidis Charalampos , Chatziprodromidou Ioanna , Floros George , Eskitzis Panagiotis , Zarogoulidis Paul , Koulouris Charilaos , Sevva Christina , Papadopoulos Konstantinos , Roulia Panagiota , Mantalovas Stylianos , Dagher Marios , Karakousis Alexandros Vasileios , Varsamis Nikolaos , Vlassopoulos Konstantinos , Theodorou Vasiliki , Mystakidou Chrysi Maria , Katsios Nikolaos Iason , Farmakis Konstantinos , Kosmidis Christoforos
Published: March 2024

Extensive research into mRNA vaccines for cancer therapy in preclinical and clinical trials has prepared the ground for the quick development of immune-specific mRNA vaccines during the COVID-19 pandemic. Therapeutic cancer vaccines based on mRNA are well tolerated, and are an attractive choice for future cancer immunotherapy. Ideal personalized tumor-dependent mRNA vaccines could stimulate both humoral and cellular immunity by overcoming cancer-induced immune suppression and tumor relapse. The stability, structure, and distribution strategies of mRNA-based vaccines have been improved by technological innovations, and patients with diverse tumor types are now being enrolled in numerous clinical trials investigating mRNA vaccine therapy. Despite the fact that therapeutic mRNA-based cancer vaccines have not yet received clinical approval, early clinical trials with mRNA vaccines as monotherapy and in conjunction with checkpoint inhibitors have shown promising results. In this review, we analyze the most recent clinical developments in mRNA-based cancer vaccines and discuss the optimal platforms for the creation of mRNA vaccines. We also discuss the development of the cancer vaccines’ clinical research, paying particular attention to their clinical use and therapeutic efficacy, which could facilitate the design of mRNA-based vaccines in the near future.
Investigating the Effects of Mixing Dynamics on Twin-Screw Granule Quality Attributes via the Development of a Physics-Based Process Map
Authors: Kotamarthy Lalith , Karkala Subhodh , Dan Ashley , Román-Ospino Andrés D. , Ramachandran Rohit
Published: March 2024

Twin-screw granulation (TSG) is an emerging continuous wet granulation technique that has not been widely applied in the industry due to a poor mechanistic understanding of the process. This study focuses on improving this mechanistic understanding by analyzing the effects of the mixing dynamics on the granule quality attributes (PSD, content uniformity, and microstructure). Mixing is an important dynamic process that simultaneously occurs along with the granulation rate mechanisms during the wet granulation process. An improved mechanistic understanding was achieved by identifying and quantifying the physically relevant intermediate parameters that affect the mixing dynamics in TSG, and then their effects on the granule attributes were analyzed by investigating their effects on the granulation rate mechanisms. The fill level, granule liquid saturation, extent of nucleation, and powder wettability were found to be the key physically relevant intermediate parameters that affect the mixing inside the twin-screw granulator. An improved geometrical model for the fill level was developed and validated against existing experimental data. Finally, a process map was developed to depict the effects of mixing on the temporal and spatial evolution of the materials inside the twin-screw granulator. This process map illustrates the mechanism of nucleation and the growth of the granules based on the fundamental material properties of the primary powders (solubility and wettability), liquid binders (viscosity), and mixing dynamics present in the system. Furthermore, it was shown that the process map can be used to predict the granule product quality based on the granule growth mechanism.
Impact of a Mock OSCE on Student Confidence in Applying the Pharmacists’ Patient Care Process
Authors: Cristobal Eleonso , Perkins Kathryn , Kang Connie , Chen Steven
Published: March 2024

The Medical and Pharmacy Student Collaboration (MAPSC) student organization at the University of Southern California, Alfred E. Mann School of Pharmacy and Pharmaceutical Sciences, created an extracurricular, peer-led, virtual group mock objective structured clinical examination (MOSCE) to expose first-year pharmacy students (P1s) to the Pharmacists’ Patient Care Process (PPCP). The purpose of this study is to evaluate the impact of a MAPSC MOSCE on P1s self-reported confidence in applying the PPCP and on patient communication, medication knowledge, and clinical skills. An anonymous, optional, self-reported survey was administered to P1s before and after the event, where they rated their confidence on a scale of 0–100 (0 = not confident, 100 = certainly confident). The statistical analysis was a paired two-tailed t-test with a significance level of p < 0.05. A total of 152 P1s and 30 facilitators attended the MOSCE. One hundred thirty-nine students met the inclusion criteria and were included in the data analysis. There was a statistically significant difference in the change in self-reported confidence for all PPCP components and learning outcomes. The results of our study strongly indicate that introducing P1 students to the PPCP through a MAPSC MOSCE format is a valuable experience.
Problems of Connectionism
Authors: Vassallo Marta , Sattin Davide , Parati Eugenio , Picozzi Mario
Published: March 2024

The relationship between philosophy and science has always been complementary. Today, while science moves increasingly fast and philosophy shows some problems in catching up with it, it is not always possible to ignore such relationships, especially in some disciplines such as philosophy of mind, cognitive science, and neuroscience. However, the methodological procedures used to analyze these data are based on principles and assumptions that require a profound dialogue between philosophy and science. Following these ideas, this work aims to raise the problems that a classical connectionist theory can cause and problematize them in a cognitive framework, considering both philosophy and cognitive sciences but also the disciplines that are near to them, such as AI, computer sciences, and linguistics. For this reason, we embarked on an analysis of both the computational and theoretical problems that connectionism currently has. The second aim of this work is to advocate for collaboration between neuroscience and philosophy of mind because the promotion of deeper multidisciplinarity seems necessary in order to solve connectionism’s problems. In fact, we believe that the problems that we detected can be solved by a thorough investigation at both a theoretical and an empirical level, and they do not represent an impasse but rather a starting point from which connectionism should learn and be updated while keeping its original and profoundly convincing core.
Study on Speckle Noise Reduction in Laser Projection Displays
Authors: Zhang Hongyou , Hu Yu , Peng Shuihai , Liu Yong
Published: March 2024

Laser speckle has a negative effect on laser projectors, so reducing laser speckle is crucial for the development of laser projector displays. We primarily focus on studying the laser speckle contrast of laser projector displays and the mechanism for reducing speckle. Based on the theory of decreasing temporal and spatial coherence of laser light, this report derives the complete formula for calculating speckle contrast in a laser projector display and provides detailed calculation procedures. According to the comprehensive formula, the primary factors influencing speckle contrast encompass wavelength, spectrum, angles of incidence or observation of lasers, the roughness of the screen surface, the number of independent speckle patterns generated by a moving diffuser, and the number of resolution elements within one eye resolution element in the projector lens. Various methods have been used in the projection engine to suppress speckle, and the main factors for reducing speckle have been verified through theoretical calculations and experimental verification. At a testing distance of 700 mm and with an F-number of 41.7 for the detector lens, the RGB laser speckle contrasts were measured to be 9.1%, 7.3%, and 10.4%, respectively, which aligns well with the results obtained from theoretical calculations. Meanwhile, the speckle contrast of the white field was also measured, yielding a result of 5.6%. The speckle contrast becomes imperceptible when the viewing distance exceeds 2000 mm in our projection system.
Design and Optimization of Silver Nanostructured Arrays in Plasmonic Metamaterials for Sensitive Imaging Applications
Authors: Okamoto Koichi , Tanaka Daisuke , Matsuyama Tetsuya , Wada Kenji , Arima Yusuke , Tamada Kaoru
Published: March 2024

This paper delves into the strategic design and optimization of silver (Ag) nanostructured arrays within plasmonic metamaterials, targeting the enhancement of imaging sensitivity. Leveraging Finite-Difference Time-Domain (FDTD) simulations, our research rigorously compares various Ag nanostructured geometries, including nanospheres, nanocones, nanodisks, and nanocubes. The aim is to pinpoint configurations that significantly enhance electric field localization on the surfaces of nanostructures, a pivotal factor. The nanocube array exhibits superior field enhancement, particularly in narrow nanogaps, suggesting its suitability for high-sensitivity applications. Further exploration into nanocube arrays reveals the crucial role of nanogap size and spacer layer thickness in tuning the optical properties through the manipulation of Fabry–Pérot and mirror image modes in metal–insulator–metal (MIM) structures. By presenting a thorough analysis of these nanostructured arrays, the study not only contributes to our understanding of the fundamental principles governing plasmonic metamaterials but also provides a solid foundation for future innovation in highly sensitive imaging applications. It underscores the importance of nanostructure design and optimization in achieving significant improvements in the performance of plasmonic devices, marking a pivotal step forward in the field of nanophotonics and its application to sensitive imaging technologies.
Lightness Peaks during the Menstrual Phase: A Retrospective Challenge to a Visual Arousal Theory of Estrogen
Authors: Foutch Brian
Published: March 2024

(1) Background: The influence of estrogen on cognitive and perceptual functions is debated. Some research suggests that estrogen increases arousal, improving cognitive function, while others propose that increased arousal might reduce performance in certain tasks. This study investigates the effects of menstrual cycle phase and estrogen levels on lightness perception in cycling women and hormonal contraceptive (HC) users. (2) Methods: Sixteen women (nine with natural cycles and seven HC users) completed three sessions aligned with different menstrual cycle phases. During these sessions, participants adjusted the luminance of five test stimuli (representing blue, green, green-yellow, yellow, and red) until they matched a flickering reference white stimulus. Lightness was calculated as the ratio of the reference stimulus luminance (5 cd/m2) divided by the test luminance required to match. Estrogen levels were also determined for each participant from saliva samples collected on the morning of each session. The effects of wavelength and menstrual cycle phase on lightness perception were analyzed, followed by post hoc comparisons and correlations between lightness perception and estrogen levels for both cycling women and HC users. (3) Results: Lightness varied by menstrual phase (MCP) in cycling women and was slightly higher during the low estrogen menstrual phase compared to peri-ovulation or luteal phases. In HC users, lightness measures were equivalent across phases. For cycling women, lightness was negatively correlated with estrogen for the green and green-yellow stimuli. There were no such associations among HC users. (4) Conclusions: This report challenges the concept that high estrogen phases of the menstrual cycle always positively influence perception. Conversely, these results revealed that—at least in cycling, non-hormonal contraceptive users—lightness perception was both at a maximum during the low estrogen menstrual phase and negatively associated with estrogen levels across all tested wavelengths.
Adoption of the 2A Ribosomal Skip Principle to Track Assembled Virions of Pepper Mild Mottle Virus in Nicotiana benthamiana
Authors: Jiao Mengting , Yin Yueyan , Tian Yanzhen , Lei Jianing , Lin Lin , Wu Jian , Lu Yuwen , Zheng Hongying , Yan Fei , Wang Jianguang , Peng Jiejun
Published: March 2024

The coat protein (CP) is an important structural protein that plays many functional roles during the viral cycle. In this study, the CP of pepper mild mottle virus (PMMoV) was genetically fused to GFP using the foot-and-mouth disease virus peptide 2A linker peptide and the construct (PMMoV-GFP2A) was shown to be infectious. The systemic spread of the virus was monitored by its fluorescence in infected plants. Electron microscopy and immunocolloidal gold labelling confirmed that PMMoV-GFP2A forms rod-shaped particles on which GFP is displayed. Studies of tissue ultrastructure and virion self-assembly confirmed that PMMoV-GFP2A could be used to monitor the real-time dynamic changes of CP location during virus infection. Aggregations of GFP-tagged virions appeared as fluorescent plaques in confocal laser microscopy. Altogether, PMMoV-GFP2A is a useful tool for studying the spatial and temporal changes of PMMoV CP during viral infection.
The Invasive Ailanthus altissima: A Biology, Ecology, and Control Review
Authors: Soler Jordi , Izquierdo Jordi
Published: March 2024

Tree of Heaven (Ailanthus altissima (Mill.) Swingle) is a tree native to China which has invaded disturbed areas in many regions worldwide. Its presence endangers natural ecosystems by displacing native species, modifying habitats, changing community structures, and affecting ecosystem processes. Its invasive nature is enhanced by its high ability to reproduce both vegetatively through root regrowth and sexually through seeds. Seeds, which are wind dispersed, are the main mechanism by which this species reaches new habitats. When they germinate and develop the root system, roots emit new shoots that contribute to a rapid increase in the tree density and the subsequent expansion of the population nearby. The contradictory results about the ecological requirements for seeds to germinate and their degree of dormancy and longevity indicate the complexity and difficulty of understanding the mechanisms that govern the biology and adaptability of this plant. The management of this weed aims at its eradication, with programs based on herbicide applications carried out by injecting the active ingredient directly to the trunk. But, not many active ingredients have shown total control, so new ones should be tested in order to increase the range of available herbicides. During the last few decades, some biological agents have been identified, but their efficacy in controlling the tree and their safety for the local flora have not yet been determined. A correct management strategy should take into account all these aspects in order to contain the expansion of this species and, ultimately, allow its eradication.
Simultaneous Impact of Rhizobacteria Inoculation and Leaf-Chewing Insect Herbivory on Essential Oil Production and VOC Emissions in Ocimum basilicum
Authors: Palermo Tamara Belén , Cappellari Lorena del Rosario , Palermo Jimena Sofía , Giordano Walter , Banchio Erika
Published: March 2024

Inoculation with rhizobacteria and feeding by herbivores, two types of abiotic stress, have been shown to increase the production of secondary metabolites in plants as part of the defense response. This study explored the simultaneous effects of inoculation with Bacillus amyloliquefaciens GB03 (a PGPR species) and herbivory by third-instar Spodoptera frugiperda larvae on essential oil (EO) yield and volatile organic compound (VOC) emissions in Ocimum basilicum plants. The density of glandular trichomes was also examined, given that they are linked to EO production and VOC emission. Herbivory increased EO content, but inoculation on its own did not. When combined, however, the two treatments led to a 10-fold rise in EO content with respect to non-inoculated plants. VOC emissions did not significantly differ between inoculated and non-inoculated plants, but they doubled in plants chewed by the larvae with respect to their undamaged counterparts. Interestingly, no changes were observed in VOC emissions when the treatments were tested together. In short, the two biotic stressors elicited differing plant defense responses, mainly when EO was concerned. PGPR did not stimulate EO production, while herbivory significantly enhanced it and increased VOC emissions. The combined treatment acted synergistically, and in this case, PGPR inoculation may have had a priming effect that amplified plant response to herbivory. Peltate trichome density was higher in inoculated plants, those damaged by larvae, and those subjected to the combination of both treatments. The findings highlight the intricate nature of plant defense mechanisms against various stressors and hint at a potential strategy to produce essential oil through the combined application of the two stressors tested here.
Spatial and Temporal Dynamics of the Vegetation Cover from the Bijagual Massif, Boyacá, Colombia, during the 1986–2021 Period
Authors: Gil-Leguizamón Pablo Andrés , Pereña-Ortiz Jaime Francisco , Sánchez-Mata Daniel , Salvo-Tierra Ángel Enrique , Mercado-Gómez Jorge David , Morales-Puentes María Eugenia
Published: March 2024

Landscape changes based on spectral responses allow showing plant cover changes through diversity, composition, and ecological connectivity. The spatial and temporal vegetation dynamics of the Bijagual Massif from 1986 to 2021 were analyzed as a measure of ecological integrity, conservation, and territory. The covers identified were high open forest (Hof), dense grassland of non-wooded mainland (Dgnm), a mosaic of pastures and crops (Mpc), lagoons (Lag), and bare and degraded lands (Bdl). The Bijagual Massif has 8574.1 ha. In 1986, Dgnm occupied 42.6% of the total area, followed by Mpc (32.8%) and Hof (24.5%); by 2000, Mpc and Hof increased (43.7 and 28.1%, respectively), while Dgnm decreased (28%); by 2021, Dgnm was restricted to the northeastern zone and continued to decrease (25.2%), Mpc occupied 52.9%, Hof 21.7% and Bdl 0.1%. Of the three fractions of the connectivity probability index, only dPCintra and dPCflux contribute to ecological connectivity. Hof and Dgnm show patches with biota habitat quality and availability. Between 1986 and 2021, Dgnm lost 1489 ha (41%) and Hof 239.5 ha (11%). Mpc replaced various covers (1722.2 ha; 38%) in 2021. Bijagual has a valuable biodiversity potential limited by Mpc. Territorial planning and sustainable agroecological and ecotourism proposals are required due to the context of the ecosystems.
Directed Self-Assembly of Cylinder-Forming Block Copolymers Using Pillar Topographic Patterns
Authors: Huh June
Published: March 2024

We conducted a computational study on the self-assembly behavior of cylinder-forming block copolymers, directed by a guide pattern of hexagonally or tetragonally arrayed pillars, using mesoscale density functional theory simulations. By adjusting the spacing (Lp) and diameter (D) of the pillars in relation to the intrinsic cylinder-to-cylinder distance (L2) of the cylinder-forming block copolymer, we investigated the efficiency of multiple-replicating cylinders, generated by the block copolymer, through the pillar-directed self-assembly process. The simulations demonstrated that at specific values of normalized parameters L˜2=L2/Lp and D˜=D/Lp coupled with suitable surface fields, triple and quadruple replications are achievable with a hexagonally arrayed pillar pattern, while only double replication is attainable with a tetragonally arrayed pillar pattern. This work, offering an extensive structure map encompassing a wide range of possible parameter spaces, including L˜2 and D˜, serves as a valuable guide for designing the contact hole patterning essential in nanoelectronics applications.
Effect of Adding Red Propolis to Edible Biodegradable Protein Films for Coating Grapes: Shelf Life and Sensory Analysis
Authors: Filgueiras Cristina Tostes , Fakhouri Farayde Matta , Garcia Vitor Augusto dos Santos , Velasco José Ignacio , Nogueira Gislaine Ferreira , Ramos da Silva Luan , Oliveira Rafael Augustus de
Published: March 2024

Red propolis is an active ingredient of great nutritional interest which offers numerous benefits as an antioxidant and antimicrobial agent. Thus, the objective of this research was to evaluate the application of an edible and antimicrobial gelatine coating containing red propolis to increase the shelf life of grapes. Gelatine films with an addition of 5, 10, 15, 20 and 25% of red propolis extract were produced to evaluate their antimicrobial activity using the disk diffusion test in solid media. The films with 25% red propolis extract showed antimicrobial activity against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The grapes were coated with pure gelatine, without a plasticizer and with gelatine with 25% red propolis and then stored for 1, 4, 10, 19 and 25 days at temperatures of 25 °C and 5 °C. The results showed that the gelatine coating with propolis reduced the mass loss of grapes stored at 25 °C for 19 days by 7.82% and by 21.20% for those kept at 5 °C for 25 days. The pH, total titratable acidity, soluble solids and color of the grapes increased due to the ripening process. Furthermore, the sensory acceptability indexes of the refrigerated grapes with coatings were superior (>78%) to those of the control samples (38%), proving the effectiveness of the coatings in maintaining the quality of grapes during storage.
Application of Infrared Pyrolysis and Chemical Post-Activation in the Conversion of Polyethylene Terephthalate Waste into Porous Carbons for Water Purification
Authors: Efimov Mikhail , Vasilev Andrey , Muratov Dmitriy , Panin Alexander , Malozovskaya Maria , Karpacheva Galina
Published: March 2024

In this study, we compared the conversion of polyethylene terephthalate (PET) into porous carbons for water purification using pyrolysis and post-activation with KOH. Pyrolysis was conducted at 400–850 °C, followed by KOH activation at 850 °C for samples pyrolyzed at 400, 650, and 850 °C. Both pyrolyzed and post-activated carbons showed high specific surface areas, up to 504.2 and 617.7 m2 g−1, respectively. As the pyrolysis temperature increases, the crystallite size of the graphite phase rises simultaneously with a decrease in specific surface area. This phenomenon significantly influences the final specific surface area values of the activated samples. Despite their relatively high specific surface areas, pyrolyzed PET-derived carbons prove unsuitable as adsorbents for purifying aqueous media from methylene blue dye. A sample pyrolyzed at 650 °C, with a surface area of 504.2 m2 g−1, exhibited a maximum adsorption value of only 20.4 mg g−1. We propose that the pyrolyzed samples have a surface coating of amorphous carbon poor in oxygen groups, impeding the diffusion of dye molecules. Conversely, post-activated samples emerge as promising adsorbents, exhibiting a maximum adsorption capacity of up to 127.7 mg g−1. This suggests their potential for efficient dye removal in water purification applications.
Characterization of Polylactic Acid Biocomposites Filled with Native Starch Granules from Dioscorea remotiflora Tubers
Authors: Estrada-Girón Yokiushirdhilgilmara , Fernández-Escamilla Víctor Vladimir Amílcar , Martín-del-Campo Angelina , González-Nuñez Rubén , Canché-Escamilla Gonzalo , Uribe-Calderón Jorge , Tepale Nancy , Aguilar Jacobo , Moscoso-Sánchez Francisco Javier
Published: March 2024

Biocomposites were fabricated utilizing polylactic acid (PLA) combined with native starch sourced from mountain’s yam (Dioscorea remotiflora Knuth), an underexplored tuber variety. Different starch compositions (7.5, 15.0, 22.5, and 30.0 wt.%) were blended with PLA in a batch mixer at 160 °C to produce PLA/starch biocomposites. The biocomposites were characterized by analyzing their morphology, particle size distribution, thermal, X-ray diffraction (XDR), mechanical, and dynamic mechanical (DMA) properties, water absorption behavior, and color. The results showed that the amylose content of Dioscorea remotiflora starch was 48.43 ± 1.4%, which corresponds to a high-amylose starch (>30% of amylose). Particle size analysis showed large z-average particle diameters (Dz0) of the starch granules (30.59 ± 3.44 μm). Scanning electron microscopy (SEM) images showed oval-shaped granules evenly distributed throughout the structure of the biocomposite, without observable agglomeration or damage to its structure. XDR and DMA analyses revealed an increase in the crystallinity of the biocomposites as the proportion of the starch increased. The tensile modulus (E) underwent a reduction, whereas the flexural modulus (Eflex) increased with the amount of starch incorporated. The biocomposites with the highest Eflex were those with a starch content of 22.5 wt.%, which increased by 8.7% compared to the neat PLA. The water absorption of the biocomposites demonstrated a higher uptake capacity as the starch content increased. The rate of water absorption in the biocomposites followed the principles of Fick’s Law. The novelty of this work lies in its offering an alternative for the use of high-amylose mountain’s yam starch to produce low-cost bioplastics for different applications.
An Enhanced Temperature Control Approach to Simulate Profile Extrusion
Authors: Vidal João , Nóbrega João Miguel
Published: March 2024

Thermoplastic extrusion, a widely used method for processing thermoplastic materials, requires precise temperature control to ensure product quality. However, existing computer-aided engineering tools often oversimplify the temperature distribution calculations, leading to additional discrepancies between simulations and the actual processes. This study introduces a novel multi-region modeling approach to address this issue. By employing realistic temperature control conditions, the methodology overcomes the limitations of current numerical modeling tools. The key contributions include the development of a transient, incompressible, non-isothermal solver integrated into the OpenFOAM computational library and the implementation of a specialized boundary condition that emulates Proportional-Integral-Derivative (PID) control using real-time thermocouple measurements. The findings highlight temperature deviations at the flow channel walls and total pressure drop while demonstrating a smaller impact on velocity and flow uniformity at the outlet under steady-state conditions. This research substantially advances the understanding of thermal dynamics in extrusion processes, offering crucial insights for enhancing temperature control and laying the groundwork for more effective and precise operational strategies.
Modeling and Switched Control of Modular Reconfigurable Flight Array for Faulty Redundancy
Authors: Ren Bin , Yang Chunxi , Zhang Xiufeng , Mao Wenyuan
Published: March 2024

The modular reconfigurable flight array (MRFA) is composed of multiple identical flight unit modules, which has several advantages such as structural variability, strong versatility, and low cost. Due to the redundant properties of MRFA, it keeps stable by adopting a suitable control law when it suffers actuator fault or actively stops some actuators. To address the attitude stability issue of the modular flight array when actuators actively stop or encounter failures during the flight process, a modeling method based on a switched system is proposed at first, and an arbitrary switched controller design method based on the segmented Lyapunov functions and the average dwell time is also given. By introducing the actuator efficiency matrix, the dynamic switched model of the modular flight array is described. Then, a group of arbitrary switched linear feedback gains is designed to ensure the exponential stability of the flight array if the switched process satisfies the constraint of the average dwell time. Simulation and experiment results indicate that when there is an accident in the actuator states, the switched controllers can achieve precise tracking of the desired trajectory, thus confirming the effectiveness of the proposed modeling method and controller.
Research on Radial Double Velocity Measurement Method of Laser Tracker
Authors: Lv Fei , Hu Chang’an , Li Jiangang , Xu Yue
Published: March 2024

For the dynamic problem that the low-speed sliding table is unable to meet the radial measuring speed of the laser tracker, this paper takes the sliding table of the indoor large-length standard device as the moving object to double the measuring distance by adding a pyramid prism, thereby doubling the radial speed of the laser tracker. In this paper, the measurement data are analyzed through equal interval measurement experiments, equal sampling frequency experiments and repeatability measurement experiments using a pyramid prism to obtain the following conclusions, respectively: Firstly, the stability of the actual interval of the laser tracker is optimal when the rated speed of the sliding table is 50 mm/s. When the pyramid prism is not used, the minimum standard deviation obtained by the laser tracker at a sampling interval of 5 mm is 0.0158 mm. Secondly, during the equal sampling frequency measurement, the stability of the laser interferometer is better than that of the laser tracker when the pyramid prism is not used. With two instruments at a sampling frequency of 10 Hz, the standard deviations of the local velocity of the laser interferometer are 0.1466 mm/s, 0.0693 mm/s and 0.1106 mm/s, respectively. The standard deviations of the laser tracker are 0.1582 mm/s, 0.1033 mm/s and 0.2008 mm/s, respectively. The same conclusion is obtained at a sampling frequency of 20 Hz. The stability of the laser tracker is better than that of the laser interferometer when the pyramid prism is used. Thirdly, the stability and reliability of the local velocity of the laser tracker are better than that of the laser interferometer. For example, at 10 Hz, the standard deviations of the local velocity of the laser tracker are 0.2745 mm/s and 0.2097 mm/s, respectively, and the repeatability of the local velocity is 0.9140 mm/s. The standard deviations of the local velocity of the interferometer are 0.6141 mm/s and 0.6368 mm/s, respectively, and the repeatability of the local velocity is 1.4886 mm/s. And the local velocity of the two measuring instruments is more reliable and stable at a low frequency (10 Hz). This experimental scheme provides a way to measure the high speed of a laser tracker using a sliding table at low speed.
Analyzing the Effect of Extraction Parameters on Phenolic Composition and Selected Compounds in Clove Buds Using Choline Chloride and Lactic Acid as Extraction Agents
Authors: Strižincová Petra , Šurina Igor , Jablonský Michal , Majová Veronika , Ház Aleš , Hroboňová Katarína , Špačková Andrea
Published: March 2024

Utilizing a deep eutectic solvent-like mixture such as choline chloride and lactic acid in a 1:2 molar ratio, clove buds underwent extraction. Furthermore, the determination of the primary components in the clove extracts, namely eugenol, eugenol acetate, and β-caryophyllene, was conducted using the HPLC-DAD method. The total phenolic content (TPC) was also established. Extraction conditions using choline chloride and lactic acid encompassed variables such as extraction temperature (within the range of 40 to 80 °C), water addition (ranging from 5.6 to 40%), and extraction time (ranging from 30 to 90 min). Optimal operational conditions for TPC were pinpointed at 77 °C, 30 min, and a water addition of 40%. The findings showed that clove extracts obtained at 60 °C, 22.8%, and 30 min had the highest amount of eugenol (307.26 ± 8.44 mg/g dry raw material).
Non-Structural Vibro-Compressed Concrete Incorporating Industrial Wastes
Authors: Pignotti Gabriela Bertazzi , Matos Ana Mafalda , Ferreira Fernanda Giannotti da Silva
Published: March 2024

This study presents more eco-efficient concrete formulations for precast vibro-compressed masonry blocks. The proposed formulations incorporated industrial waste, glass powder (GP), and quartz powder (QP), in which natural aggregate was partially replaced by QP (10%) and Portland cement by GP (10% and 20%). The best combination of powder materials, water, and admixture was optimised at mortar level, considering a “zero slump” criteria and compressive strength. Afterwards, studies at concrete level followed. Specimens were vibrated and compressed in laboratory and immediately demoulded, aiming to simulate the industrial process. The compressive strength decreased when GP and QP were used alone; however, when combining 10% GP as cement replacement + 10% QP as fine aggregate replacement, the compressive strength increased by approximately 26.6% compared to the reference concrete. Water absorption results varied between 8.92 and 17.9%, and the lowest absorption was obtained by concrete specimens incorporating 20% GP. The UPV presented a narrow range of variation among all concrete mixtures under study, around 2–2.5 km/s at 28 days, whereas electrical resistivity was achieved at 28 days, at 20,000 and 25,000 ohms. Although there were some limitations of the casting process at the laboratory scale, the research results showed promising results, and it seems feasible to use this waste as a substitute for non-renewable raw materials in the production of concrete on an industrial scale. This can provide added value to abundant local wastes while contributing to a circular concrete economy.
Ethics Education in the Context of an Interreligious Encounter Day (IED): Empirical Research Results from Austria
Authors: Lehmann Karsten
Published: March 2024

This article adds an empirical contribution to international discussions on interreligious education, taking the point of view of the academic study of religion. It presents results from a Viennese research project that used a participant observation design to analyze one of the more recent pedagogical formats to deal with religious plurality at school—the so-called Interreligious Encounter Day (IED). More precisely this article focuses on the role of Ethics Education (EE) within the context of a specific IED that was organized in 2019. Along those lines, it highlights the ambiguity of IED practices at a school by presenting a threefold argument: first, the article makes the point that the IED opened up a space to explicitly make religion a subject of discussion at school. Second, it argues that—within the context of the IED—religion is constructed in a specific way that can be described as ‘school religion’. Third, it proposes that the observed IED format has the tendency to prioritize specific individual constructions of religion—especially those prompted by theology.
Consecrating the Peripheral: On the Ritual, Iconographic, and Spatial Construction of Sui-Tang Buddhist Corridors
Authors: Xu Zhu
Published: March 2024

The corridor-enclosed cloister characterized Buddhist monasteries during the Sui and Tang periods. This architectural form was first introduced by Emperor Liang Wudi from the palace and continued to prevail until the eleventh century, when a gradual transformation occurred, resulting in the corridor evolving into a long, narrow image hall. This paper examines the ritual and pictorial programs of the Sui-Tang Buddhist corridor to gain insight into this transformation and its ceremonial significance. Specifically, it explores how the corridor was empowered by the state-sponsored maigre feast as a place of worship and how the monastic community of a particular school appropriated the space to celebrate an unbroken dharma-transmission lineage from the Buddha to a specific group of Chinese patriarchs. Lastly, the paper aims to comprehend the adaptation of the corridor into an image hall, which was influenced by political and religious shifts in the eleventh century when Buddhist monasteries were no longer designated as the ritual arena for the state-sponsored maigre feast.
Syncretism in Exegesis: The Integration of Confucian Texts in Chengguan’s Huayan Commentary
Authors: Hamar Imre
Published: March 2024

Commentarial literature constitutes a cornerstone in the edifice of Chinese Buddhism, providing critical exegesis of Indian Buddhist texts. This paper examines the pivotal role of Chengguan (738–839), the fourth patriarch of the Huayan school, revered for his extensive commentarial work on the Chinese translations of the Buddhāvataṃsaka-sūtra. Chengguan not only composed a written commentary but also engaged in discourses with the monastic and lay communities at Wutaishan, prompting the creation of a sub-commentary derived from these oral elucidations. The study posits that the composition of Chengguan’s audiences, comprising Confucian-educated scholars and Buddhist monks, necessitated a pedagogical strategy that integrated Chinese intellectual traditions into the Buddhist narrative to enhance comprehension. This analysis focuses on Chengguan’s citations of the Analects, showcasing how he interweaves Confucian maxims into the fabric of his commentary to illuminate Buddhist doctrines. The research articulates the method he employed to make the Buddhist texts resonate with a Chinese audience.