bluuebunny/crossref_metadata_2025 huggingface dataset:
Advancing Hydroinformatics and Water Data Science Instruction: Community Perspectives and Online Learning Resources
Authors: Jones Amber Spackman , Horsburgh Jeffery S. , Bastidas Pacheco Camilo J. , Flint Courtney G. , Lane Belize A.
Published: June 2022

Hydroinformatics and water data science topics are increasingly common in university graduate settings through dedicated courses and programs as well as incorporation into traditional water science courses. The technical tools and techniques emphasized by hydroinformatics and water data science involve distinctive instructional styles, which may be facilitated by online formats and materials. In the broader hydrologic sciences, there has been a simultaneous push for instructors to develop, share, and reuse content and instructional modules, particularly as the COVID-19 pandemic necessitated a wide scale pivot to online instruction. The experiences of hydroinformatics and water data science instructors in the effectiveness of content formats, instructional tools and techniques, and key topics can inform educational practice not only for those subjects, but for water science generally. This paper reports the results of surveys and interviews with hydroinformatics and water data science instructors. We address the effectiveness of instructional tools, impacts of the pandemic on education, important hydroinformatics topics, and challenges and gaps in hydroinformatics education. Guided by lessons learned from the surveys and interviews and a review of existing online learning platforms, we developed four educational modules designed to address shared topics of interest and to demonstrate the effectiveness of available tools to help overcome identified challenges. The modules are community resources that can be incorporated into courses and modified to address specific class and institutional needs or different geographic locations. Our experience with module implementation can inform development of online educational resources, which will advance and enhance instruction for hydroinformatics and broader hydrologic sciences for which students increasingly need informatics experience and technical skills.
Relationship Between Irrigation Thresholds and Potato Tuber Depth in Sandy Soil
Authors: Matteau Jean-Pascal , Celicourt Paul , Shahriarina Elnaz , Letellier Philipe , Gumiere Thiago , Gumiere Silvio J.
Published: June 2022

Soil disturbance resulting from tuber crop harvesting is a major threat to soil health. The depth of soil intervention is a critical factor that further strengthens the effects of such disturbance and makes harvest one of the most harmful cropping operations. In the case of potato, soil moisture is a determining factor for root and stolon development, hence, a deeper soil intervention may be required at harvest. While potato ranks as the fourth most cultivated crop worldwide, the impact of soil moisture on potato tuber vertical and horizontal distribution has received very little attention. The objective of this study was to evaluate the effects of four soil matric potential thresholds (SMPTs; –10, –20, –30, and –45 kPa) on the spatial (vertical and horizontal) distribution of potato tubers grown in plastic containers filled with sandy soil using an X-ray computed tomography scanner. The results of the experiments conducted in a greenhouse environment suggest that the horizontal distribution of the tubers did not differ significantly across the irrigation treatments. However, a linear relationship between SMPT, and therefore irrigation threshold, and potato tuber depth was observed. In addition, the deepest tuber position was observed under the –10 kPa SMPT, while the tubers were closer to the soil surface under the –45 kPa SMPT, which could lead to a greater preponderance of tuber diseases such as late blight or greening. Thus, potato irrigation events implementing a SMPT between –20 and –30 kPa could reduce the harvest depth, hence, decreasing the negative impacts of soil disturbance on soil structural stability and soil organic carbon degradation while mitigating the impacts of disease as well as reducing fuel costs, greenhouse gas emissions, soil loss and erosion.
Combination of Autophagy Selective Therapeutics With Doxil: An Assessment of Pathological Toxicity
Authors: Helke Kristi L. , Gudi Radhika R. , Vasu Chenthamarakshan , Delaney Joe R.
Published: June 2022

Background: Combination therapy of targeted drugs in cancer treatment is a field in constant flux, with research balancing side effects with efficacy. Efficacy from combination therapy is improved either through synthetic lethality or through prevention of recurrent clones. Previous research has shown (hydroxy-)chloroquine is insufficient to disrupt autophagy in tumors. Hence, either combinations or novel autophagy agents are desired. In vivo studies of ovarian cancer have revealed that chloroquine can be combined with up to four other autophagy drugs to suppress ovarian cancer growth. While cancer efficacy is now established for the autophagy drug combination, it is unclear what toxicities may require monitoring in human trials. Additive toxicity with chemotherapy is also unknown.

Methods: To address toxicity in more depth than previous weight-monitoring studies, biochemical and histopathology studies were performed. Mouse groups were treated with autophagy drugs for 2 weeks, with or without the chemotherapy Doxil. After the last dose, mice were processed for blood biochemistry, white blood cell markers, and histopathology.

Results: Data from a comprehensive blood biochemistry panel, flow cytometric measurements of blood cell markers, and histopathology are herein reported. While Doxil presented clear bone marrow and immunologic toxicity, autophagy drugs were overall less toxic and more variable in their presentation of potential toxicities. Only minor additive effects of autophagy drugs with Doxil were observed.

Conclusion: Combinations of autophagy drugs may be considered for therapy in human oncology trials, with possible side effects to monitor informed by these murine pre-clinical data.
Commensal Lactobacilli Enhance Sperm Qualitative Parameters in Dogs
Authors: Mahiddine Feriel Yasmine , You Inhwan , Park Heekee , Kim Min Jung
Published: June 2022

Although several methods have been developed to improve male fertility and sperm quality, subfertility remains a primary clinical issue in male reproduction worldwide. The aim of this study was to determine the effects of the oral administration of three commensalLactobacillusspp. on healthy normozoospermic dogs and the qualitative parameters of their sperm. Three weeks of supplementation induced a significant decrease of two phyla, Proteobacteria and Tenericutes, and an increase of phylum Firmicutes. At the species level, the number ofFusobacterium perfoetensandAnaerobiospirillum succiniciproducensdecreased, whileLimosilactobacillus reuteriincreased. Parallel to these results, qualitative sperm parameters such as total and progressive motility, acrosome integrity, and other kinematic parameters were significantly enhanced after commensal lactobacilli supplementation. In addition, we showed that Firmicutes were positively correlated with sperm qualitative parameters, while Proteobacteria,F. perfoetens, andA. succiniciproducenswere negatively correlated. Considering the similarities between the gut microbiome of dogs and humans, these results provide more insight into how gut microbiota regulation could improve male sperm quality in both species.
Recipient Comorbidities for Prediction of Primary Graft Dysfunction, Chronic Allograft Dysfunction and Survival After Lung Transplantation
Authors: Ehrsam Jonas Peter , Schuurmans Macé M. , Laager Mirjam , Opitz Isabelle , Inci Ilhan
Published: June 2022

Since candidates with comorbidities are increasingly referred for lung transplantation, knowledge about comorbidities and their cumulative effect on outcomes is scarce. We retrospectively collected pretransplant comorbidities of all 513 adult recipients transplanted at our center between 1992–2019. Multiple logistic- and Cox regression models, adjusted for donor-, pre- and peri-operative variables, were used to detect independent risk factors for primary graft dysfunction grade-3 at 72 h (PGD3-T72), onset of chronic allograft dysfunction grade-3 (CLAD-3) and survival. An increasing comorbidity burden measured by Charleston-Deyo-Index was a multivariable risk for survival and PGD3-T72, but not for CLAD-3. Among comorbidities, congestive right heart failure or a mean pulmonary artery pressure >25 mmHg were independent risk factors for PGD3-T72 and survival, and a borderline risk for CLAD-3. Left heart failure, chronic atrial fibrillation, arterial hypertension, moderate liver disease, peptic ulcer disease, gastroesophageal reflux, diabetes with end organ damage, moderate to severe renal disease, osteoporosis, and diverticulosis were also independent risk factors for survival. For PGD3-T72, a BMI>30 kg/m2 was an additional independent risk. Epilepsy and a smoking history of the recipient of >20packyears are additional independent risk factors for CLAD-3. The comorbidity profile should therefore be closely considered for further clinical decision making in candidate selection.
Learning-Based Online QoE Optimization in Multi-Agent Video Streaming
Authors: Wang Yimeng , Agarwal Mridul , Lan Tian , Aggarwal Vaneet
Published: June 2022

Video streaming has become a major usage scenario for the Internet. The growing popularity of new applications, such as 4K and 360-degree videos, mandates that network resources must be carefully apportioned among different users in order to achieve the optimal Quality of Experience (QoE) and fairness objectives. This results in a challenging online optimization problem, as networks grow increasingly complex and the relevant QoE objectives are often nonlinear functions. Recently, data-driven approaches, deep Reinforcement Learning (RL) in particular, have been successfully applied to network optimization problems by modeling them as Markov decision processes. However, existing RL algorithms involving multiple agents fail to address nonlinear objective functions on different agents’ rewards. To this end, we leverage MAPG-finite, a policy gradient algorithm designed for multi-agent learning problems with nonlinear objectives. It allows us to optimize bandwidth distributions among multiple agents and to maximize QoE and fairness objectives on video streaming rewards. Implementing the proposed algorithm, we compare the MAPG-finite strategy with a number of baselines, including static, adaptive, and single-agent learning policies. The numerical results show that MAPG-finite significantly outperforms the baseline strategies with respect to different objective functions and in various settings, including both constant and adaptive bitrate videos. Specifically, our MAPG-finite algorithm maximizes QoE by 15.27% and maximizes fairness by 22.47% compared to the standard SARSA algorithm for a 2000 KB/s link.
Fumigant Toxicity and Feeding Deterrent Activity of Essential Oils from Lavandula dentata, Juniperus procera, and Mentha longifolia against the Land Snail Monacha obstructa
Authors: Abobakr Yasser , Al-Sarar Ali S. , Abdel-Kader Maged S.
Published: June 2022

Land mollusks are one of the most destructive agricultural pests worldwide, the management of which depends on synthetic molluscicides. However, many of these molluscicides are harmful to nontarget organisms. Hence, there is a need to develop alternative ecofriendly molluscicides that are less impactful toward nontarget organisms. So, an investigation into the fumigant toxicity and feeding deterrent effect of essential oils (EOs) from Lavandula dentata L. (Lamiaceae), Juniperus procera Hochst. (Cupressaceae), and Mentha longifolia (L.) Huds. (Lamiaceae) against the land snail Monacha obstructa (Pfeiffer, 1842) (Hygromiidae) was performed. L. dentata EO exhibited the highest fumigant toxicity with LC50 values of 8.68 μL/L air and 7.24 μL/L air after 24 h and 48 h exposure periods, respectively. Its main components were camphor, 1,8-cineole, fenchone, and β-myrecene. The fumigant toxicity of J. procera EO was lower than that of L. dentata, with LC50 values of 25.63 μL/L air and 20.11 μL/L air after 24 h and 48 h exposure periods, respectively. The major constituents of J. procera EO were α-pinene, p-cymene, and β-ocimene. The analysis of M. longifolia EO showed that pulegone, and menthol were the major constituents. However, it displayed no fumigant toxicity up to 50 μL/L air. The three EOs exhibited a strong feeding deterrent effect at sublethal concentrations. The EOs extracted from L. dentata, J. procera and M. longifolia are promising ecofriendly botanical molluscicides against the land snail M. obstructa.
Response of Potted Citrus Trees Subjected to Water Deficit Irrigation with the Application of Superabsorbent Polyacrylamide Polymers
Authors: Cea Daniela , Bonomelli Claudia , Mártiz Johanna , Gil Pilar M.
Published: June 2022

Searching for new strategies to mitigate the effects of low water availability for citrus production, a study was carried out on potted mandarin cv. W. Murcott, with the objective of evaluating the physiological and growth response of the plants to polyacrylamide gel application in the substrate in water restriction conditions. The following treatments were evaluated, T0 (control) with 100% ETc water replenishment, T1 with 50% ETc water replenishment, and T2 with 50% ETc water replenishment plus the application of polyacrylamide polymers to the substrate. Temperature and water volumetric content (ϴ: m3 m−3) were evaluated in the substrate. Plant water-status parameters such as stem water potential (SWP), stomatal conductance (gs), and chlorophyll fluorescence (Fv/Fm), as well as biomass, nutrients levels, and proline biosynthesis were measured in the plants in response to the treatments. The results showed that the substrate moisture for T2 was kept significantly higher than T0 and T1, despite receiving the same irrigation rate as T1 and a half of T0; however, this higher moisture availability in the substrate of T2 was not reflected in the plant’s water status or growth. On the contrary, the T2 plants showed responses such as lower total biomass, lower vegetative development, and lower root biomass, as well as a higher concentration of proline in the root. According to these results, it is concluded that polymers such as polyacrylamide sodium allow the retention of water in the substrate, but do not necessarily release that water for plants, probably because that moisture is kept in the hydrogel and not released to the substrate media or the roots, or if released, in this case, this occurs with an increase in the concentration of sodium available to the plants, which could lead the citrus crop to a worse situation of water and/or osmotic stress.
The Circular Economy as an Axis of Agricultural and Rural Development: The Case of the Municipality of Almócita (Almería, Spain)
Authors: Corral Francisco Javier García , Vázquez Rosa María Martínez , García Juan Milán , de Pablo Valenciano Jaime
Published: June 2022

In recent years, the concept of the circular economy has been gaining relevance and its importance has grown both in academia and in rural municipalities in general. The rural development policy of the European Union, in addition to prioritizing the diversification of the productive activities of municipalities, encourages the adoption of the circular economy. The aim of this article is to show and publicize the applications relating to the circular economy that are being carried out in a rural mountain municipality with a small population focused on agriculture, and which are setting an example for others that are suffering the endemic problem of depopulation. A diagnosis is carried out taking into consideration local sustainable development methodologies. In relation to the results, the positive impact of these practices with a rural development approach based on awareness and education regarding the basic 3Rs (reduce, reuse, recycle) is highlighted. Almócita is an example that can be extrapolated to many mountain municipalities at national and international levels.
Spermidine Modify Antioxidant Activity in Cucumber Exposed to Salinity Stress
Authors: Korbas Agata , Kubiś Jan , Rybus-Zając Magdalena , Chadzinikolau Tamara
Published: June 2022

The effects of short-term 48 h long NaCl-stress and spermidine level modification on polyamines level and antioxidant status in cucumber (Cucumis sativus cv. Dar) leaves were investigated. Seedlings kept in nutrient solutions treated with 50 mM NaCl for 48 h exhibited reduced relative water content and accumulation of free polyamines, especially spermidine. Salinity stress caused an increase in superoxide radicals and hydrogen peroxide generation during the salinity-induced increase in antioxidant enzyme activities. Spermidine application before stress resulted in a marked increase in spermidine and spermine contents in the leaves of salt-stressed cucumber seedlings. Additionally, increased spermidine/spermine level mobilised the antioxidant enzyme’s activity and limited reactive oxygen species content. Polyamine synthesis inhibitor (MGBG) slightly decreased spermidine and spermine levels during salinity and reversed the antioxidant activity mobilisation. These results showed that Spd modifications significantly improved PAs, enhancing salinity stress tolerance by detoxifying ROS. Our findings determined the implication of PAs for improving the salinity tolerance of important vegetable species.
Determination of Minimum Doses of Imazamox for Controlling Xanthium strumarium L. and Chenopodium album L. in Bean (Phaseolus vulgaris L.)
Authors: Gürbüz Ramazan , Yentürk Ömer
Published: June 2022

This study was conducted to investigate the minimum doses of the imazamox active ingredient (ai) that provide satisfactory efficacy (>90%) against fat hen (Chenopodium album L.) and common cocklebur (Xanthium strumarium L.). These two weeds are among the most troublesome weeds of bean fields. The minimum dose studies were carried out separately in the 2–4 and 6–8 true leaf stages of both weeds. The experiments were carried out in pots under greenhouse conditions. The experiments were repeated three times. In the first two experiments, the recommended dose of imazamox (100%) together with 75%, 50% and 25% doses were applied to the weeds during the above-mentioned leaf stages. Some pots were left untreated for control. In the third experiments, 12.50% and 6.25% of the recommended doses were also tested. Plant height and the number of leaves were recorded on the 1st, 3rd, 5th, 7th, 14th, 21st and 28th days following the herbicide application. As a result of the studies, it was determined that nearly half the recommended dose (48.18 g ai/da) provides 90% success in the control of common cocklebur (X. strumarium) when applied at the 2–4 true leaf stages, while a lower dose (36.11 g ai/da) is required for obtaining the same control when applied at the 6–8 true leaf stages. For the fat hen (C. album), only a 17.69 g ai/da application dose was found to provide 90% control at the period of 2–4 true leaves, while 21.21 g ai/da was noted to provide 90% control when applied at the 6–8 true leaf stage. The results suggest that the increase in leaf area reduces the imazamox requirement for the control of X. strumarium.
Biochar Mitigated Yield-Scaled N2O and NO Emissions and Ensured Vegetable Quality and Soil Fertility: A 3-Year Greenhouse Field Observation
Authors: Zhang Xi , Zhang Qianqian , Xu Xintong , Dong Yubing , Xiong Zhengqin
Published: June 2022

Biochar amendments have been widely used in agricultural soil for lowering N2O and NO emissions while improving soil fertility and crop performance. However, a thorough understanding of the longevity of the favorable effects would be a prerequisite for large-scale biochar application in agriculture. We conducted a three-year greenhouse vegetable trial in Southeast China to systematically investigate the impacts of biochar mixed with nitrogen (N) on soil N2O and NO emissions, vegetable performance, and soil fertility at an interannual scale. The field experiment was established in November 2016 with biochar (0, 20 and 40 t ha−1; C0, C1, and C2, respectively), applied once without/with 240 kg N ha−1 urea (N0 or N1, respectively). Soil N2O and NO emissions were monitored during the spring vegetable cultivation period, and vegetable yield, quality, and soil properties were measured after harvests in 2018, 2019, and 2020. Results indicated that N application significantly increased N2O and NO emissions and vegetable yield throughout the trial period. Biochar combined with N generally reduced N2O and NO emissions and emission factors while increasing the vegetable yield, leading to lower yield-scaled N2O and NO emissions in 2018 and 2019. Biochar markedly enhanced soil pH and organic carbon and persisted, but generally had no significant effect on N use efficiency (NUE), vegetable quality, and soil fertility index (SFI) among treatments in over-fertilized vegetable fields. Based on our results, biochar application at 20 t ha−1 combined with N seemed to achieve the highest agronomic and environmental benefits for intensive vegetable production in Southeast China.
Effect of the Forecast Air Temperature Change on the Water Needs of Vines in the Region of Bydgoszcz, Northern Poland
Authors: Jagosz Barbara , Rolbiecki Stanisław , Rolbiecki Roman , Ptach Wiesław , Sadan Hicran A. , Kasperska-Wołowicz Wiesława , Pal-Fam Ferenc , Atilgan Atilgan
Published: June 2022

The climatic changes observed in Poland are manifested by an increase in air temperature, but not an increase in rainfall, which causes an increase in the water needs of plants, and hence the necessity to develop irrigation systems for crops. The aim of this study was to assess the water needs of grapevines in 2021–2050 in the Bydgoszcz region (northern Poland), an area with high requirements for supplementary irrigation. The calculations were based on the expected changes in air temperature according to the climate change scenario for Poland: the 4th Intergovernmental Panel on Climate Change—Special Report on Emissions Scenarios: balanced scenario (A1B). The average monthly temperature in 1981–2010 was used in the calculations. The water needs of grapevines were estimated using the reference evapotranspiration calculated using the Blaney–Criddle equation, and then using the crop coefficient and potential (crop) evapotranspiration. It was found that in 2021–2050, the water needs of grapevines, both during the growing season (May–September) and during the irrigation period (June–August), will increase by 6%. The highest and significant increase in water needs, by 10%, should be expected in August. The estimated increase in water needs of grapevines indicates the need to development sustainable irrigation systems for vineyards in northern Poland.
Modulation of Antioxidant Defense, Immune Response, and Growth Performance by Inclusion of Propolis and Bee Pollen into Broiler Diets
Authors: AL-Kahtani Saad N. , Alaqil Abdulaziz A. , Abbas Ahmed O.
Published: June 2022

(1) Background: Propolis and bee pollen have natural bioactive compounds that may support the performance and immunological response of broilers. (2) Methods: The study included 300 1 d old Cobb-500 broiler chicks. Starting from 22–42 d of age, chicks were divided according to a 2 × 2 factorial design into one of the four treatment groups (5 replicates × 15 chicks per replicate); a basal diet without supplementation (CONT) or supplemented with 1 g/kg of propolis (PR) or bee pollen (BP) separately or in an even combination (PR + BP). (3) Results: A significant (p < 0.05) increase was obtained in the body-weight gain of broilers treated with PR, BP, and PR + BP compared to the CONT. The total antioxidant capacity and superoxide dismutase were highly (p < 0.05) activated in all treated groups compared to the CONT. Immunological parameters, especially the leukocyte cell viability, T- and B-lymphocyte proliferation, immunoglobulins (IgA and IgM), antibody titers, and wattle-swelling test were significantly (p < 0.05) enhanced in the treated broilers with PR and/or BP compared to the CONT. (4) Conclusions: The dietary supplementation of PR and/or BP could be beneficial for broiler growth through maximizing the antioxidant- and immune-system defenses.
Apex and ApeTouch: Development of a Portable Touchscreen System and Software for Primates at Zoos
Authors: Martin Christopher Flynn , Muramatsu Akiho , Matsuzawa Tetsuro
Published: June 2022

We report on the development and testing of a portable touchscreen apparatus and accompanying software program for primate enrichment, cognitive research, and husbandry applications. For zoos considering using technology to bolster scientific efforts or enhance the welfare of primates in their care, touchscreen activities offer a solution that has a long and proven record of primate use in laboratory settings as well as a history of usage in the zoo world. We review the options that are available for zoos to build their own touchscreen systems and we offer as an alternative our pre-built apparatus, Apex, and primate software suite, ApeTouch, both of which are tailored for use in a zoo setting. The efficacy and utility of these tools are demonstrated in a training study with four macaque groups of different species that were previously naïve to touchscreens. All of the groups in the study learned to use the device and displayed a consistent engagement with the touchscreen tasks over 95 daily sessions of exposure. In the final stage of the training, two of the four groups displayed an above-chance level performance on a numerical sequencing task.
Effects of Vine Tea Extract on Meat Quality, Gut Microbiota and Metabolome of Wenchang Broiler
Authors: Zhou Luli , Li Hui , Hou Guanyu , Wang Jian , Zhou Hanlin , Wang Dingfa
Published: June 2022

This study investigates the effects of vine tea (Ampelopsis grossedentata) extract (AGE) on meat quality, gut microbiota and cecal content metabolites of Wenchang broilers. A total of 240 female Wenchang broilers aged 70 days were randomly allocated into four groups with five replicates of twelve broilers each. Broilers were fed a corn-soybean basal diet supplemented with AGE at 0 (T1), 0.2% (T2), 0.4% (T3) and 0.6% (T4) until 124 days of age. The whole feeding trial lasted 54 days. Results suggest that the content of total triglycerides and low-density lipoprotein cholesterol in serum of broilers are linearly reduced with dietary AGE supplementation (p < 0.05). The T3 and T4 groups had higher (p < 0.05) a* value in thigh and breast muscles than the T1 group. Additionally, the dietary supplementation of AGE decreased the shear force and drip loss of both thigh and breast muscles linearly (p < 0.05). Compared with the T1 group, AGE supplementation increased the levels of inosine monophosphate (IMP) significantly (p < 0.05) in both the thigh and breast muscles. Furthermore, an increase (p < 0.05) in the total unsaturated fatty acid (USFA), polyunsaturated fatty acids (PUFA) and the ratio of unsaturated fatty acids to saturated fatty acid (USFA: SFA) in both the thigh and breast muscles in the T3 group was observed. Higher abundance of Bacteroidota (p < 0.05) and lower abundance of Firmicutes (p < 0.05) were observed in the T3 group. The abundance of Faecalibacterium was significantly decreased (p < 0.05) in the T3 group compared with the T1 group. Cholesterol sulfate and p-cresol sulfate were identified as differential metabolites between the T1 and T3 groups. It suggested that 0.4% of AGE supplementation significantly downregulated the levels of p-cresol sulfate and cholesterol sulfate (p < 0.05) and the hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) activity compared with the control. Our present study demonstrates that dietary supplementation with AGE can improve the quality and flavor by increasing the IMP and PUFA content in the muscle of Wenchang broilers. Furthermore, dietary AGE supplementation with 0.4% can regulate the cholesterol metabolism of Wenchang broilers.
Delayed First Milking in Unassisted Overnight Calving Did Not Affect the Quality of Colostrum but Influenced Serum Brix Refractometry in Holstein Calves at Two Days of Life
Authors: Manosalva Daniel Gustavo , Grispoldi Luca , Spagnolo Marco , Crociati Martina
Published: June 2022

Timely administration of good-quality colostrum represents the first farm strategy to avoid the failure of passive transfer (FPT). However, calves born during the night are likely to be fed later than recommended. Our aim was to evaluate whether night-occurring calving and delayed first milking affected colostrum quality and immune passive transfer. The dataset included 463 calvings. Four liters of colostrum were administered by an esophageal tube feeder. The mean Brix% of colostrum was 27.43%, while serum Brix% at two days of life in calves was 10.19%. According to the Generalized Linear Model, parity ≥ 4, calving months of March, April, and from September to November positively influenced the quality of colostrum. Dams carrying a male calf produced lower quality colostrum compared with those carrying a female calf (−2.78 ± 1.04 Brix%, p = 0.008); heavier female calves were associated with greater colostrum quality (0.29 ± 0.05 for each kg increase, p < 0.001). Night- or day-calving had no effect on the quality of colostrum. The only factor influencing the serum Brix% of female Holstein calves at two days of life was the day- or night-occurring birth (−0.386 ± 0.188 Brix% in calves born during the night, p = 0.04). Our results showed that calves born overnight and fed the day after had decreased serum Total Protein concentrations as indicated by reduced Brix refractometer readings, compared with calves born during the day and fed quickly after birth. However, the administration of 4 L of high-quality colostrum likely improved their serum Brix% at two days of life. Alternatively, where the prevalence of good-quality colostrum is lower, improving calving supervision and ensuring timely feeding are important to reduce the risk of FPT.
Mycobacterioses Induced by Mycobacterium abscessus: Case Studies Indicating the Importance of Molecular Analysis for the Identification of Antibiotic Resistance
Authors: Ryskova Lenka , Bolehovska Radka , Kukla Rudolf , Svarc Michal , Zavrelova Alzbeta , Vanicek Hubert , Pavlik Ivo , Bostik Pavel
Published: June 2022

Mycobacterioses are less frequently occurring but serious diseases. In recent years, at a global level, the incidence of mycobacterioses induced by the rapidly growing species Mycobacterium abscessus (M. a.), which is considered to be the most resistant to antibiotics and most difficult to treat, has been on the rise. Correct identification to the level of the subspecies (M. a. abscessus, M. a. massiliense, and M. a. bolletii) and determination of its sensitivity to macrolides, which are the basis of combination therapy, are of principal importance for the management of the disease. We describe five cases of mycobacterioses caused by M. a., where the sequencing of select genes was performed to identify the individual subspecies and antibiotic resistance. The analysis of the rpoB gene showed two isolates each of M. a. abscessus and M. a. massiliense and one isolate of M. a. bolletii. The complete (full length) erm(41) gene responsible for the development of inducible resistance to macrolides was demonstrated in both M. a. abscessus and M. a. bolletii isolates. A partially deleted and non-functional erm(41) gene was demonstrated in M. a. massiliense isolates. The subsequent sequencing of the full length erm(41) gene products showed, however, the mutation (T28→C) in both isolates of M. a. abscessus, causing a loss of the function and preserved sensitivity to macrolides. The antibiotic sensitivity testing confirmed that both the isolates of M. a. abscessus and M. a. massiliense were sensitive to clarithromycin even after prolonged 14-day incubation. The inducible resistance to clarithromycin was maintained only in M. a. bolletii. Thus, the sequence analysis of the erm(41) gene can reliably identify the preservation of sensitivity to macrolides and serve as an important tool in the establishment of therapeutic regimens in cases of infections with M. abscessus.
Seminal Plasma Antioxidants Are Related to Sperm Cryotolerance in the Horse
Authors: Catalán Jaime , Yánez-Ortiz Iván , Tvarijonaviciute Asta , González-Aróstegui Luis Guillermo , Rubio Camila P. , Barranco Isabel , Yeste Marc , Miró Jordi
Published: June 2022

The objective of this study was to determine the relationship of enzymatic (superoxide dismutase, SOD; glutathione peroxidase, GPX; catalase, CAT; and paraoxonase type 1, PON1) and non-enzymatic antioxidants (measured in terms of: Trolox equivalent antioxidant capacity, TEAC; cupric-reducing antioxidant capacity, CUPRAC; and ferric-reducing ability of plasma, FRAP), as well as the oxidative stress index (OSI) in seminal plasma (SP) with the resilience of horse sperm to freeze-thawing. Twenty-one ejaculates (one per individual) were collected and split into two aliquots: the first was used to harvest the SP and assess the activity levels of antioxidants and the OSI, and the second one was cryopreserved. The following post-thaw sperm quality parameters were evaluated: sperm motility, plasma membrane and acrosome integrity, mitochondrial membrane potential, intracellular levels of reactive oxygen species (ROS), and plasma membrane lipid disorder. Based on post-thaw total motility (TM) and plasma membrane integrity (SYBR14+/PI−), ejaculates were hierarchically (p < 0.001) clustered into two groups of good (GFE) and poor (PFE) freezability. The SP activity levels of PON1, SOD, and TEAC were higher (p < 0.05) in GFE than in PFE, showing a positive relationship (p < 0.05) with some sperm motility parameters and with plasma membrane (PON1 and TEAC) and acrosome (SOD and TEAC) integrity. In contrast, OSI was higher (p < 0.05) in the SP of PFE than in that of GFE, and was negatively correlated (p < 0.05) to some sperm motility parameters and to plasma membrane and acrosome integrity, and positively (p < 0.05) to the percentage of viable sperm with high plasma membrane lipid disorder. In conclusion, enzymatic (PON1 and SOD) and non-enzymatic (TEAC) antioxidants of SP are related to horse sperm cryotolerance. In addition, our results suggest that PON1 could be one of the main antioxidant enzymes involved in the control of ROS in this species. Further investigation is needed to confirm the potential use of these SP-antioxidants and OSI to predict sperm cryotolerance in horses.
Phytochemical and Safety Evaluations of Finger Lime, Mountain Pepper, and Tamarind in Zebrafish Embryos
Authors: Cáceres-Vélez Paolin Rocio , Ali Akhtar , Fournier-Level Alexandre , Dunshea Frank R. , Jusuf Patricia Regina
Published: June 2022

Plants play a pivotal role in drug discovery, constituting 50% of modern pharmacopeia. Many human diseases, including age-related degenerative diseases, converge onto common cellular oxidative stress pathways. This provides an opportunity to develop broad treatments to treat a wide range of diseases in the ageing population. Here, we characterize and assess the toxicological effects of finger lime (Citrus australasica), mountain pepper (Tasmannia lanceolata), and small-leaved tamarind (Diploglottis australis) extracts. The characterization demonstrates that these Australian native plants have antioxidant potential and, importantly, they have high concentrations of distinct combinations of different antioxidant classes. Using zebrafish larvae as a high-throughput pre-clinical in vivo toxicology screening model, our experiment effectively discriminates which of these extracts (and at what exposure levels) are suitable for development towards future therapies. The LC50-96h for finger lime and tamarind were >480 mg/L, and 1.70 mg/L for mountain pepper. Critically, this work shows that adverse effects are not correlated to the properties of these antioxidants, thus highlighting the need for combining characterization and in vivo screening to identify the most promising plant extracts for further development. Thus, we present a high-throughput pre-clinical screening that robustly tests natural plant products to utilize the diversity of antioxidant compounds for drug development.
Bioactive Compounds from the Zingiberaceae Family with Known Antioxidant Activities for Possible Therapeutic Uses
Authors: Alolga Raphael N. , Wang Feizuo , Zhang Xinyao , Li Jia , Tran Lam-Son Phan , Yin Xiaojian
Published: June 2022

The Zingiberaceae family is a rich source of diverse bioactive phytochemicals. It comprises about 52 genera and 1300 species of aromatic flowering perennial herbs with characteristic creeping horizontal or tuberous rhizomes. Notable members of this family include ginger (Zingiber officinale Roscoe), turmeric (Curcuma longa L.), Javanese ginger (Curcuma zanthorrhiza Roxb.), and Thai ginger (Alpinia galanga L.). This review focuses on two main classes of bioactive compounds: the gingerols (and their derivatives) and the curcuminoids. These compounds are known for their antioxidant activity against several maladies. We highlight the centrality of their antioxidant activities with notable biological activities, including anti-inflammatory, antidiabetic, hepatoprotective, neuroprotective, antimicrobial, and anticancer effects. We also outline various strategies that have been applied to enhance these activities and make suggestions for research areas that require attention.
Ferroptosis and Its Emerging Role in Pre-Eclampsia
Authors: Chen Zhixian , Gan Jianfeng , Zhang Mo , Du Yan , Zhao Hongbo
Published: June 2022

Iron is essential for cell survival, and iron deficiency is a known risk factor for many reproductive diseases. Paradoxically, such disorders are also more common in cases of iron overload. Here, we evaluated the role of ferroptosis in women’s health, particularly focusing on pre-eclampsia (PE). PE is a multisystem disorder and is one of the leading causes of maternal and perinatal morbidity and mortality, especially when the condition is of early onset. Nevertheless, the exact etiological mechanism of PE remains unclear. Interestingly, ferroptosis, as a regulated iron-dependent cell death pathway, involves a lethal accumulation of lipid peroxides and shares some characteristics with PE pathophysiology. In this review, we comprehensively reviewed and summarized recent studies investigating the molecular mechanisms involved in the regulation and execution of ferroptosis, as well as ferroptosis mechanisms in the pathology of PE. We propose that ferroptosis not only plays an important role in PE, but may also become a novel therapeutic target for PE.
Immunomodulatory Effects of Aronia Juice Polyphenols—Results of a Randomized Placebo-Controlled Human Intervention Study and Cell Culture Experiments
Authors: Lackner Sonja , Sconocchia Tommaso , Ziegler Tobias , Passegger Christina , Meier-Allard Nathalie , Schwarzenberger Elke , Wonisch Willibald , Lahousen Theresa , Kohlhammer-Dohr Alexandra , Mörkl Sabrina , Derler Martina , Strobl Herbert , Holasek Sandra Johanna
Published: June 2022

Dietary polyphenols, which are present in Aronia melanocarpa, have been associated with various beneficial effects on human health including antioxidant, antiviral, and anti-inflammatory activities. We aimed to investigate the immunomodulatory effects of aronia juice polyphenols in a randomized placebo-controlled human intervention study and cell culture experiments. A total of 40 females were asked to consume either 200 mL of aronia juice or a placebo drink for six weeks and were investigated again after a washout period of another six weeks. We observed that only half of the participants tolerated the aronia juice well (Vt) and the other half reported complaints (Vc). The placebo (P) was generally tolerated with one exception (p = 0.003). Plasma polyphenol levels increased significantly in Vt after the intervention (p = 0.024) but did neither in P nor in Vc. Regulatory T cell (Treg) frequencies remained constant in Vt and P during the intervention, whereas Tregs decreased in Vc (p = 0.018). In cell culture, inhibiting effects of ferulic acid (p = 0.0005) and catechin (p = 0.0393) on the differentiation of Tregs were observed as well as reduced activation of CD4-T cells in ferulic acid (p = 0.0072) and aronia juice (p = 0.0163) treated cells. Interestingly, a CD4+CD25−FoxP3+ cell population emerged in vitro in response to aronia juice, but not when testing individual polyphenols. In conclusion, our data strengthen possible individual hormetic effects, the importance of the food matrix for bioactivity, and the need for further investigations on possible impacts of specific physiological features such as the gut microbiota in the context of personalized nutrition.
3,4′,5-Trimethoxy-trans-stilbene Alleviates Endothelial Dysfunction in Diabetic and Obese Mice via Activation of the AMPK/SIRT1/eNOS Pathway
Authors: Zhou Chunxiu , Tan Yi , Xu Baojun , Wang Yitao , Cheang Wai-San
Published: June 2022

3,4′,5-trimethoxy-trans-stilbene (TMS) is a methoxylated derivative of resveratrol. Previous studies showed the vaso-protective effects of resveratrol; nevertheless, research on this derivative is scarce. The current study aimed to explore whether TMS can alleviate endothelial dysfunction in diabetic and obese mice, along with the underlying mechanisms. Thoracic aortas isolated from male C57BL/6J mice and primary cultures of rat aortic endothelial cells were treated with high glucose with or without TMS. High glucose exposure impaired acetylcholine-induced endothelium-dependent relaxations, down-regulated NO bioavailability and the AMP-activated protein kinase (AMPK)/Sirtuin 1 (SIRT1)/endothelial nitric oxide synthase (eNOS) pathway, increased endoplasmic reticulum (ER) stress and oxidative stress, which were reversed by TMS treatment. Moreover, the protective effects of TMS were abolished by Compound C (AMPK inhibitor), and EX527 (SIRT1 inhibitor). The mice were fed with high-fat diet (60% kcal% fat) for 14 weeks to establish a diabetic and obese model, and were orally administered TMS (10 mg/kg/day) in the last 4 weeks. Chronic TMS treatment alleviated endothelial dysfunction via enhancing the AMPK/SIRT1/eNOS pathway and attenuated oxidative stress and ER stress in aortas of diet-induced obese mice. In summary, our study reveals the potent vaso-protective effect of TMS and its therapeutic potential against endothelial dysfunction in metabolic disorders.
Phase Behavior and Ionic Conductivity of Blended, Ion-Condensed Electrolytes with Ordered Morphologies
Authors: Collins Hannah , Liu Jiacheng , Yang Lingyu , Schaefer Jennifer L.
Published: June 2022

In this study, the amphiphilic salt lithium trifluoromethanesulfonylimide octadecane (C18LiTFSI) was used as a basis to investigate the effects of anion density and cation coordination sites within blended electrolytes with strong ionic aggregation. C18LiTFSI was previously reported as a single-component, ion-condensed electrolyte with a wide layered liquid crystalline phase regime. Three additive molecules with varyingly sized polar sulfonyl groups attached to an octodecane-tail were synthesized and mixed with C18LiTFSI. The thermal properties, morphology, and ionic conductivity of the blended electrolytes were characterized. It was found that the blended electrolytes exhibited layered liquid crystalline morphology over a narrower temperature range than the pure salt, and the ionic conductivity of the blended liquid crystalline electrolytes were generally lower than that of the pure salt. Surprising, the additives were found to have the greatest effect on the bulk ionic conductivity of the semicrystalline phase of the electrolytes. Addition of minor fractions of methylsulfonyloctadecane to C18LiTFSI resulted in increases in conductivity of over two orders of magnitude at room temperature, while addition of ethylsulfonyloctadecane or isopropylsulfonyloctadecane with the larger head group resulted in decreased ionic conductivity over the entire composition space and temperature range investigated.
Development of Screen-Printable Nafion Dispersion for Electrochemical Sensor
Authors: Chen Zun , Patel Rishi , Berry Jacob , Keyes Chloe , Satterfield Camron , Simmons Cody , Neeson Alec , Cao Xi , Wu Qihua
Published: June 2022

A modified Nafion dispersion for direct screen printing was developed and characterized. Commercial Nafion solution was neutralized to its sodium form and the solvent was replaced by a screen-printing-compatible solvent. The modified Nafion dispersion has optimized rheological properties including shear-thinning behavior, thixotropy, and moderate yield stress. The elemental composition and chemical states of the modified Nafion were characterized by X-ray photoelectron spectroscopy (XPS). The chemical state and composition of the modified Nafion remained the same as those of commercial Nafion. The crystallinity of the cured membranes of both Nafion dispersions was evaluated by X-ray diffraction spectroscopy (XRD). It was found that the modified Nafion has lower crystallinity as compared to the commercial Nafion, and the degree of crystallinity increases with an increase in the curing temperature. The modified material was screen printed onto a commercial sensor as a cation-exchange membrane for the detection of lead Pb(II) in buffer solutions. The sensor showed good linearity in the range of 5 µg/L to 500 µg/L, with a detection limit of 2 µg/L for Pb(II) by square-wave anodic stripping voltammetry. This work demonstrates the possibility of printing Nafion on a large scale in a wide range of fields, such as printed electrochemical sensors.
A Simple Relationship to Estimate Parameters of the Optimum Compaction Point
Authors: Maqsoud Abdelkabir
Published: June 2022

Compacted clay covers have been the most commonly used materials for hydraulic barrier layers. During their construction, the control of some parameters such as compactive effort and molding water content is required. These last parameters affect the hydraulic conductivity, which is considered as one of the important key parameters for cover design. To reach the target in terms of hydraulic conductivity, the cover must be compacted to a pre-determined dry unit weight that usually corresponds to a certain percentage of the maximum dry unit weight (γdmax). During the prefeasibility stage of a project (before conducting the required tests), γdmax and the optimum water content (wOpt) can be estimated to obtain an early overview of the conditions that can be anticipated. In this regard, a new approach was proposed to estimate these parameters using the liquid limit (wL) and the plastic limit (wP). The proposed equations were developed using data from 56 compacted clay liners and validated using 44 others taken from published data. Results presented in this paper indicate how the proposed equations can successfully estimate parameters of the optimum compacted point.