bluuebunny/crossref_metadata_2025 huggingface dataset:
Desarrollo de una red de internet de las cosas para la comunicación de un robot modular
Authors: Fernando Luis , Alberto Henry , Cadena Ernesto
Published: July 2024

El internet de las cosas (internet of things, más conocido como IoT) es una tecnología que permite conectar entre sí a los dispositivos de un espacio geográfico limitado como una casa u organización. Esta tecnología tiene aplicación en el campo de la robótica porque permite inspeccionar y administrar de forma remota las operaciones de dispositivos y es una herramienta clave para suplir las necesidades de la comunicación como la cobertura, la fiabilidad y el tiempo de respuesta. La red de área extensa de baja potencia —una tecnología IoT emergente para la red de comunicaciones en el campo de la robótica— es relevante porque permite comunicar el computador, que contiene el algoritmo para el control del movimiento de un robot, con los módulos de un robot, ya que no se requiere de una alta velocidad de transmisión, es de largo alcance y tiene bajo consumo energético.Los autores de este libro desarrollaron una red de comunicaciones con dispositivos de IoT para supervisar y coordinar el desplazamiento de un robot de múltiples módulos con el uso de una red de área extensa de baja potencia. El libro presenta los antecedentes de los sistemas de comunicación usados en los robots, los tipos de robots modulares, un análisis de las redes de área extensa de baja potencia, el algoritmo desarrollado para la coordinación y control de los movimientos del robot, la técnica para la comunicación del robot modular con la nube, y la evaluación del sistema de comunicaciones y sus parámetros de calidad de servicio. Uno de los principales aportes del proyecto es la medición y análisis de los parámetros de calidad de servicio en este tipo de red de comunicaciones.
Igualdade de género e Direitos Humanos no Brasil e na União Europeia No Século XXI
Authors: Xavier João Proença , Cruz Giovana
Published: July 2024

Desde 10 de Dezembro de 1948 com a criação da Declaração Universal dos Direitos Humanos (DUDH) pressupõe-se institucionalmente que todos os indivíduos devem ser tratados igualmente perante a lei e a sociedade. Várias leis sobre o direito das mulheres, crianças, adolescentes, idosos nos mostram que essa igualdade precisa de reconhecimento para ser aplicada. As leis sobre os Direitos humanos das mulheres são resultado da busca por tratamento igualitário e por respeito a suas decisões, os acordos e relatórios, movimentos ativistas existentes revelam que essa luta por reconhecimento é constante. Um fato conhecido é que a participação da mulher na sociedade atual é resultado de uma trajetória de lutas e conquistas no decorrer dos anos. O que temos hoje, ainda que seja necessário um grande caminho a ser percorrido, é devido à luta constante pelo reconhecimento igualitário entre homens e mulheres. Essa busca pela igualdade reflete preconceitos, descrenças que ainda hoje existem e que refletem a discriminação da mulher pelo simples fato de ser mulher. No presente trabalho queremos demonstrar como essa busca por direitos igualitários se desenvolveu ao longo da história, trazendo as diferentes perspectivas jurídicas existentes entre Brasil e União Europeia onde participação da mulher na sociedade atual reflete uma trajetória de lutas e conquistas.
Concepções da Bioética Hermenêutica
Authors: Lima Júnior Paulo Gomes de
Published: July 2024

O Artigo propõe uma releitura da bioética frente aos direitos humanos, em especial a genética humana. O tema do trabalho se justifica, frente aos avanços da engenharia genética e a modificação na sociedade, no meio ambiente e na humanidade, fazendo surgir questionamentos até pouco tempo inimagináveis. As terapias gênicas trouxeram renovadas esperanças de curas para doenças que, até então, eram incuráveis podendo citar, por exemplo, a utilização da decodificação do genoma humano no tocante à pandemia do COVID19. Os avanços da Engenharia Genética, em especial o mapeamento do genoma, são indispensáveis para a cura e tratamento de doenças que afligem a humanidade, devendo a bioética se preocupar com o aspecto técnico e ético dos procedimentos. De um lado, os avanços da engenharia genética trouxeram esperanças para cura, tratamento e melhoria na qualidade de vida. Por outro lado, trouxeram angústias, incertezas e discriminações que devem ser analisadas cuidadosamente. A bioética deve assegurar os avanços da engenharia genética por meio dos valores dos direitos humanos, assegurando o acesso aos procedimentos a toda humanidade. A bioética deve assegurar a ética deontológica, base para os direitos humanos, o valor universal dos direitos humanos diatópico, com respeito à humanidade universal e a cada cultura. O objetivo do trabalho é a reformulação dos valores da bioética a partir de uma genealogia crítica da ética. O trabalho objetivará colaborar com os debates e procedimentos éticos na atividade dos médicos, membros de comitê de ética, advogados e juízes, demonstrando que as suas atividades devem estar relacionadas com os direitos humanos, assegurando os avanços da Engenharia Genética por meio da valoração dos seres humanos. O trabalho utilizará no seu desenvolvimento a pesquisa dogmática ou instrumental, por meio da doutrina, jurisprudência e legislação com o intuito de observar a coerência do ordenamento jurídico frente à biotecnologia e a Engenharia Genética. A pesquisa sociojurídica irá observar as incoerências do sistema jurídico na proteção da dignidade humana a partir de suas limitações, realizando relações com outros sistemas de saberes como bioética, biotecnologia e Engenharia Genética.
ANÁLISE DO CONTRATO DE TRANSFERÊNCIA DE TECNOLOGIA
Authors: Vieira Bruno , Siqueira Maria , Aguiar Paulo
Published: July 2024

This paper is an analytical study of the Technology Transfer Agreement and its applicability in the legal system. This analysis is of great relevance to academia, providing a better understanding of its application in Brazil. Its analysis exposes the dynamism of the subject in the legal system. In this way, we can see the occasions when there is an incidence of norms that make application possible. Therefore, from a legalistic point of view, the legal foundations that justify its application in the resolution of a dispute will be shown. Finally, the aim is to analyze the subject from a historical, legal and social perspective, through the central importance that technology, as property and as a commodity, has for the development of the contemporary market and human consumer and work activities.
DATA PROTECTION IN INDIA: PRIVACY, PERSONAL DATA, AND THE SAGA OF A LEGISLATIVE AND ECONOMICAL APPROACH
Authors: Tuteja Acharaj Kaur , Singh Digvijay
Published: July 2024

Set to become the most populated country, India has always lacked an adequate legal framework for protecting the data privacy of its citizens. While random attempts have been sprung at the Parliament from time to time, there still remains an astonishing lacuna in that aspect. The same has been tried to bridge through the recent Data Protection Bill, 2022 with its 30 clauses articulated for the supposed benefit of data principals. The following research paper, takes the assistance of secondary research to delve into the economic analysis of the Bill and how it strategically contradicts the purpose and objective of its creation that is – data privacy protection. Through adoption of Coase theorem and game theory, this paper seeks to elaborate on the provisions of the Bill and how they lack an appropriate economic trade-off for most individuals involved. Right to privacy was deemed a fundamental right in 2017 itself, and the project outlines the disruption of this Apex Court judgement from an economic point of view.
ENHANCING EARLYCHILDHOOD SPEECH DEVELOPMENT THROUGH DIGITAL TECHNOLOGIES: A SYSTEMATIC REVIEW
Authors: Abisheva Saltanat , Mirza Natalya
Published: July 2024

The rapid integration of digital technologies into educational structures significantly changes traditional methods of speech development in early childhood. This systematic review examines the field of digital tools and their application in supporting language acquisition and the development of speech skills in young children. Based on selected empirical and theoretical studies published from 2020 to 2024, this article synthesizes the results from publications in peer-reviewed journals to analyze the impact, benefits and problems of using digital technologies in speech development in early childhood. The main conclusions show the significant role of interactive applications, games and advanced technologies in expanding vocabulary, pronunciation accuracy and communicative competence. In addition, the review highlights the role of parental involvement in the digital space to ensure balanced and high-quality interaction with digital content. Despite the positive results, the article also lists potential drawbacks, including concerns about screen time and the educational quality of available apps. In conclusion, the review suggests a reasonable integrationof digital technologies into early development of young children, focusing on the need for high-quality content, parental guidance and balance with interaction in the real world
Isolation, conventional and molecular identification of Fusarium proliferatum responsible to bulb rot of garlic and potential biological control by new bacterial strains
Authors: El Barnossi Azeddin , Moubchir Tarik , Beniaich Ghada , Saghrouchni Hamza , Allali Aimad , Iraqi Housseini Abdelilah
Published: July 2024

The purpose of the present study was to isolate and identify Fusarium proliferatum from infected garlic bulbs, using conventional and molecular identification techniques, as well as to evaluate the in vitro effect of new bacterial strains on the mycelial growth of F. proliferatum. Infected garlic bulb samples were collected and the phytopathogenic fungi isolated and identified using standardised conventional and molecular methods. The effect of the bacterial strains isolated from the different substrates and the filtrates of their liquid culture on the mycelial growth of F. proliferatum was studied using the direct confrontation method between F. proliferatum and the microorganism or the filtrate that may be antagonistic. The results obtained showed a precise identification of F. proliferatum responsible for garlic bulb rot and the deposit of the nucleotide sequence in the NCBI database under accession number OP820542. Three bacterial isolates with a significant inhibitory effect on the growth of F. proliferatum were isolated and selected. These isolates were identified as Bacillus subtilis (Isolat A4), Bacillus tequilensis (Isolat A11) and Pseudomonas sp. (Isolat A12) which exhibited inhibition percentages of 54.00 ± 1.00 %, 59.00 ± 1.00 %, 67.33 ± 1.70 % respectively in comparison with fluconazole 59.17 ± 0.76 %. The current study will contribute to the understanding of garlic bulb rot and to the exploration of new methods of biological control of this disease, as part of a strategy of sustainability and pesticide reduction.
Penyuluhan Digital Marketing Kepada Anggota Sekolah Pencetak Wirausaha (SPW) Binaan UMKM
Authors: Darmi Hendro , Manalu Hosanna A. R. , Samastha Vita , D. H. Ahmad Khairi , Simanulang Ratna I. H. , Rusilowati Umi
Published: July 2024

This community service aims to provide information and knowledge about how to increase Digital Marketing excellence. Digital marketing is the process of marketing and promoting a brand, product or service which is carried out through digital media and requires an internet network for its activities. Digital marketing is a marketing or promotional activity for a brand or product using digital media or the internet with the aim of attracting consumers or potential consumers quickly. The digital era refers to a period of time in which digital technology, especially the internet, significantly influences various aspects in various areas of human life. Digital technology plays an important role in changing the way we interact, access the internet, business transformation, education and learning, technological innovation, digital economy and social influence. The digital era has become integrated with the current condition of society. The development of the digital world is currently a trend and has become a necessity in all circles. The large number of SMEs that still experience difficulties in the product marketing process is an obstacle in developing their business. The aim of this activity is to increase the capabilities and knowledge of small and medium businesses through digital marketing to market a wider range of products so that sales can increase. Counseling like this needs to be carried out more frequently to build awareness and understanding of digital marketing and the steps that must be taken to create and manage social media accounts. The results of this community trial show the public's interest seen from the responses and questions submitted by the public about how to increase the advantages of MSMEs with digital marketing promotions and being able to do it consistently
Meningkatkan Kecerdasan Finansial Melalui Pelatihan dan Workshop Keuangan Pada Sekolah Pencetak Wirausaha (SPW)
Authors: Silaen Eniwawan , Novitasyari Ayu , Sonawi Sonawi , Nuraeni Eni , Putri S Theresia , Sahroni Sahroni , Sunardi Nardi
Published: July 2024

The objective of this activity is to provide practical training and knowledge on financial management for entrepreneurial development, impacting the welfare of SPW Supported by SMKN 1 Pacet Cianjur. This activity is conducted using survey methods and direct material delivery, as well as simulations and discussions on financial management in entrepreneurial development, which impacts the welfare of SPW Supported by SMKN 1 Pacet Cianjur. The conclusion drawn from this community service is that through the Community Service Program (PKM), SPW Supported by SMKN 1 Pacet Cianjur gains knowledge on improving understanding and practical skills in financial management, awareness of risks and opportunities, increased self-confidence, as well as the establishment of relationships and networks, leading to significant changes in financial behavior.
Pelatihan Literasi Keuangan Digital Bagi Binaan UMKM
Authors: Reksono Bayu , Sunjayani Dinar Novandia , Nurhayati Fitri , Nurhayati Fitri , Sampurnaningsih Sri Retnaning , Zulfitra Zulfitra
Published: July 2024

Tujuan yang ingin dicapai dari pengabdian masyarakat ini adalah memberikan wawasan tentang literasi keuangan digital pada binaan UMKM SMK Negeri 1 Pacet Cianjur.  Metode yang digunakan adalah pendekatan kepada instansi terkait yaitu siswa melalui workshop dan sosialisasi serta pelatihan berupa penjelasan penerapan ilmu manajemen dalam pengetahuan literasi keuangan digital terhadap perilaku konsumtif binaan UMKM SMK Negeri 1 Pacet Cianjur. Kesimpulan dari pengabdian kepada masyarakat ini adalah bahwa melalui program Pengabdian Kepada Masyarakat (PKM), Binaan UMKM SMK Negeri 1 Pacet Cianjur memperoleh pengetahuan mengenai peningkatan literasi keuangan digital generasi Z, dan diharapkan terhindar dari kejahatan siber digital dan pinjaman online dengan bunga tinggi.
Caracterização dos cardápios de meios de hospedagem quanto à oferta de opções para pessoas com alergias e intolerâncias alimentares
Authors: Silva Elga Batista da , Marques Anderson Campêlo
Published: July 2024

O presente trabalho buscou investigar a disponibilidade de opções para pessoas com alergias alimentares e/ou intolerâncias alimentares nos cardápios dos meios de hospedagem em canais online. Foram encontrados 16 cardápios, por meio de busca remota nos principais sites de reservas. Sobre o número de opções de alimentos e bebidas presentes nesses menus verificou-se ampla variedade de alimentos e bebidas (1441 opções de alimentos e 1167 de bebidas), sendo 224 opções de alimentos e uma de bebida sem alérgenos; nove restaurantes inseriram ícones com informações para pessoas com alergias ou intolerâncias alimentares em seus cardápios.  Tais conteúdos podem evitar a exposição do consumidor aos alimentos causadores de alergias ou intolerâncias alimentares, pois possibilitam a identificação de alérgenos nas opções do cardápio. Observou-se a necessidade de adequações nos serviços de alimentação para pessoas que demandam uma alimentação diferenciada, e a criação de processos para garantir a segurança do alimento desse público, bem como informá-lo sobre as composições dos pratos e bebidas.
Monopólio, Carestia e “Carne Virada”: A notícia de um protesto popular na Baixa dos Sapateiros no ano de 1919
Authors: Santos Elenilson Da Silva , De Sousa Junior Vilson Caetano
Published: July 2024

A carne é um gênero alimentício presente com frequência na mesa dos soteropolitanos. O consumo se estende da colônia ao império, transcendendo a república e permanecendo até os dias atuais. Muitos desafios foram enfrentados pelo povo para garantir carne de qualidade na mesa. O presente artigo traz parte desta história, no episódio onde os soteropolitanos tiveram que enfrentar uma tentativa de venda de carne deteriorada na Baixa dos Sapateiros no ano de 1919. Através da análise documental abordaremos questões sobre o abastecimento da cidade, legislação e outros assuntos correspondentes à venda de carne bovina neste período.
Bolo de aniversário com audiodescrição: acessibilidade e comensalidade para pessoas com deficiência visual
Authors: Castelo Juliana Féres , Mattoso Verônica de Andrade
Published: July 2024

O bolo de aniversário é um elemento simbólico do ritual gastronômico que perpassa gerações e culturas. Com o objetivo de investigar a relevância deste produto na vida de pessoas com deficiência visual, abordando a a acessibilidade na gastronomia, realizou-se uma pesquisa de campo por meio de um questionário semiestruturado seguido de entrevistas projetivas. As histórias e memórias sobre o bolo associado aos próprios aniversários demonstraram a relevância deste elemento gastronômico na vida das trinta e três pessoas com deficiência visual que participaram do estudo. As quais, apesar do conhecimento sobre a audiodescrição (AD), jamais haviam tido acesso à AD aplicada a bolos de aniversário e, após conhecerem, enfatizaram o potencial informativo deste recurso. Assim, sugerimos que o recurso passe a ser aplicado para promoção da acessibilidade comunicacional e para o conhecimento e identificação não somente de bolos de aniversário, mas de diversos outros produtos, para além dos gastronômicos.
Desafios e oportunidades da pesquisa científica em Gastronomia no Brasil
Authors: Ferro Rafael Cunha
Published: July 2024

Esta pesquisa teve como objetivo examinar os desafios e oportunidades para o amadurecimento do campo científico da Gastronomia a partir da sua interface com o campo científico do Turismo & Hospitalidade (T&H) no Brasil. Para tanto, entrevistas foram conduzidas com pesquisadores expoentes dessa interface. Os principais resultados, analisados a partir de uma ótica bourdiesiana, apontaram a presença de forças de pressão antagônicas, denominadas como “forças de dominação” e “forças heréticas”, que restringem ou estimulam o amadurecimento da Gastronomia enquanto um campo científico próprio e mais autônomo em relação ao T&H. As principais “forças de dominação” recaíram na constatada dependência das estruturas epistemológicas e formais que o campo científico do T&H oferece e nas deficiências teórico-metodológicas e incoerências conceituais. Entre as principais “forças heréticas” estão a proposição de curso de mestrado em Gastronomia, a criação de revistas científicas especializadas, o crescimento da comunidade científica e a amplitude desse campo. Como principais contribuições, essa pesquisa sugere algumas proposições visando o amadurecimento do campo científico da Gastronomia.
Multicultural Organizational Culture
Authors: Ramadhani Ashri
Published: July 2024

This study examines multiculturalism related to morals in a nation to do something that has become a mutual agreement and then also examines the activities of communication, negotiation, decision making and multicultural teams in a diverse organization. The type of research used in this study is library research with a qualitative approach method. The result of this study is that intercultural communication is a process of conveying messages, information, ideas or feelings between people of different cultural backgrounds. Negotiation is a dynamic and diverse process of interaction and communication, containing art and full of secrets, to achieve a goal of the parties. Intercultural decision making needs to be done because of the difference between expectations or goals and the results achieved. Multiculturalism can simply be understood as an acknowledgment that a country or society is diverse and plural
The Effect of Self Service Technology and Service Quality Toward Customer Satisfaction of Bank Nagari Syariah Batusangkar Branch
Authors: Putri Tania Ananda , Shabri Husni
Published: July 2024

The purpose of this study was to analyze the effect of self service technology and service quality on customer satisfaction of Bank Nagari Syariah Batusangkar Branch. The research method uses a quantitative approach. The population of this study consisted of 970 people who used the M-Banking application with a sample of 91 people. The analysis technique uses multiple linear regression analysis. The results showed that the variables of self service technology and service quality simultaneously influenced customer satisfaction. Partially self service technology and service quality also affect customer satisfaction at Bank Nagari Syariah Batusangkar Branch which uses the M-Banking application. The results of this study will implement bank management to be able to improve service and quality in order to maintain customer satisfaction.
Materials de recolzament 1 de l’assignatura Formes d’Organització Administrativa (36727)
Authors: Pardo Beneyto Gonzalo
Published: July 2024

El present material de recolzament ha sigut produït gràcies al projecte d’innovació educativa emergent: Learning by doing: generación de materiales innovadores para el aprendizaje en Formas de Organización Administrativa [PIEE_2023-2735093]. Així este document de suport per a l'assignatura "Formes d'Organització Administrativa" (FOA) examina el conceptes principals dels primers quatre temes de l’assignatura. Entre les temàtiques que tracta destaca el paper de l’Administració dins el sistema polític, l’anàlisi de les administracions públiques, les seues estructures, funcions i processos, així com la seva evolució a Espanya. A més se centra a les relacions entre la burocràcia, els actors polítics i els grups d’interèsi als paradigmes neoempresarials, entre altres.
Inventory of cinematic spaces. A fictional library
Authors: Barba Rosa
Published: July 2024

When I refer to speculation, I mean a zone of reflection and discussion where hypotheses can be built up. A speculation zone exists to bridge real gaps in knowledge through imagining and inventing possible explanations for baffling phenomena. One notable scholar who leaned on the idea of mixing fact, confirmed knowledge and speculation was philosopher and mathematician Imre Lakatos. His text Proofs and Refutations is written as a dialogue between fictional students and a teacher as a way to prove and disprove mathematical formulas. Lakatos argues that mathematics is a dynamic process and that proofs and discoveries are not final and immutable.
Six Sigma vs. other quality improvement tools A comparative analysis of trends in years 1985-2024
Authors: Nakielski Marcin , Ludwig Anna
Published: July 2024

Six Sigma is a widely adopted method in various industries that is aimed at process improvement and quality management. Understanding Six Sigma's evolving interest and utilization can provide valuable insights into its current significance and prospects. Using data from Google Trends, Google Books, Web of Science, and Scopus, this study examined the search volumes and interests in keywords and phrases that were related to Six Sigma over a specified period. The global analysis revealed the overall direction of interest in Six Sigma worldwide, highlighting periods of peak interest and potential significant shifts in the method’s popularity. By identifying those times with the highest concentrations of interest, the article provides a deeper understanding of the adoption and perception of Six Sigma. On top of this, Six Sigma was compared in popularity (by trends) with other known methods such as Lean, Kaizen, PDCA, and TQM. This research contributes to the existing body of knowledge by shedding light on the current trends and future directions of Six Sigma globally. The findings offer valuable insights for practitioners, researchers, and organizations that seek to leverage Six Sigma for process improvements and quality management.
Statistical examination of shared loci in neuropsychiatric diseases using genome-wide association study summary statistics
Authors: Spargo Thomas P , Gilchrist Lachlan , Hunt Guy P , Dobson Richard JB , Proitsi Petroula , Al-Chalabi Ammar , Pain Oliver , Iacoangeli Alfredo
Published: July 2024

Continued methodological advances have enabled numerous statistical approaches for the analysis of summary statistics from genome-wide association studies. Genetic correlation analysis within specific regions enables a new strategy for identifying pleiotropy. Genomic regions with significant ‘local’ genetic correlations can be investigated further using state-of-the-art methodologies for statistical fine-mapping and variant colocalisation. We explored the utility of a genome-wide local genetic correlation analysis approach for identifying genetic overlaps between the candidate neuropsychiatric disorders, Alzheimer’s disease, amyotrophic lateral sclerosis, frontotemporal dementia, Parkinson’s disease, and schizophrenia. The correlation analysis identified several associations between traits, the majority of which were loci in the human leukocyte antigen (HLA) region. Colocalisation analysis suggested that disease-implicated variants in these loci often differ between traits and, in one locus, indicated a shared causal variant between amyotrophic lateral sclerosis and Alzheimer’s disease. Our study identified candidate loci that might play a role in multiple neuropsychiatric diseases and suggested the role of distinct mechanisms across diseases despite shared loci. The fine-mapping and colocalisation analysis protocol designed for this study has been implemented in a flexible analysis pipeline that produces HTML reports and is available at: https://github.com/ThomasPSpargo/COLOC-reporter.
Enkephalin-mediated modulation of basal somatic sensitivity by regulatory T cells in mice
Authors: Aubert Nicolas , Purcarea Madeleine , Novarino Julien , Schopp Julien , Audibert Alexis , Li Wangtianrui , Fornier Marie , Cagnet Léonie , Naturel Marie , Casrouge Armanda , Dieu-Nosjean Marie-Caroline , Blanchard Nicolas , Dietrich Gilles , Peirs Cedric , Marodon Gilles
Published: July 2024

CD4
+
CD25
+
Foxp3
+
regulatory T cells (Treg) have been implicated in pain modulation in various inflammatory conditions. However, whether Treg cells hamper pain at steady state and by which mechanism is still unclear. From a meta-analysis of the transcriptomes of murine Treg and conventional T cells (Tconv), we observe that the proenkephalin gene (
Penk
), encoding the precursor of analgesic opioid peptides, ranks among the top 25 genes most enriched in Treg cells. We then present various evidence suggesting that
Penk
is regulated in part by members of the TNF receptor family and the transcription factor Batf. Using mice in which the promoter activity of
Penk
can be tracked with a fluorescent reporter, we also show that
Penk
expression is mostly detected in Treg and activated Tconv in non-inflammatory conditions in the colon and skin. Functionally, Treg cells proficient or deficient for
Penk
suppress equally well the proliferation of effector T cells
in vitro
and autoimmune colitis
in vivo
. In contrast, inducible ablation of
Penk
in Treg leads to heat hyperalgesia in both male and female mice. Overall, our results indicate that Treg might play a key role at modulating basal somatic sensitivity in mice through the production of analgesic opioid peptides.
Combined forces of hydrostatic pressure and actin polymerization drive endothelial tip cell migration and sprouting angiogenesis
Authors: Kondrychyn Igor , Wint Haymar , He Liqun , Betsholtz Christer , Phng Li-Kun
Published: July 2024

Cell migration is a key process in the shaping and formation of tissues. During sprouting angiogenesis, endothelial tip cells invade avascular tissues by generating actomyosin-dependent forces that drive cell migration and vascular expansion. Surprisingly, ECs can still invade if actin polymerization is inhibited. In this study, we show that endothelial tip cells employ an alternative mechanism of cell migration that is dependent on Aquaporin (Aqp)-mediated water inflow and increase in hydrostatic pressure. In the zebrafish, ECs express
aqp1a.1
and
aqp8a.1
in newly formed vascular sprouts in a VEGFR2-dependent manner. Aqp1a.1 and Aqp8a.1 loss-of-function studies show an impairment in intersegmental vessels formation because of a decreased capacity of tip cells to increase their cytoplasmic volume and generate membrane protrusions, leading to delayed tip cell emergence from the dorsal aorta and slower migration. Further inhibition of actin polymerization resulted in a greater decrease in sprouting angiogenesis, indicating that ECs employ two mechanisms for robust cell migration
in vivo
. Our study highlights an important role of hydrostatic pressure in tissue morphogenesis.
Follicular helper- and peripheral helper-like T cells drive autoimmune disease in human immune system mice
Authors: Vecchione Andrea , Khosravi-Maharlooei Mohsen , Danzl Nichole , Li Hao Wei , Nauman Grace , Madley Rachel , Waffarn Elizabeth , Winchester Robert , Ruiz Amanda , Ding Xiaolan , Fousteri Georgia , Sykes Megan
Published: July 2024

Human immune system (HIS) mice constructed in various ways are widely used for investigations of human immune responses to pathogens, transplants and immunotherapies. In HIS mice that generate T cells
de novo
from hematopoietic progenitors, T cell-dependent multisystem autoimmune disease occurs, most rapidly when the human T cells develop in the native NOD.Cg-
Prkdc
scid
Il2rg
tm1Wjl
(NSG) mouse thymus, where negative selection is abnormal. Disease develops very late when human T cells develop in human fetal thymus grafts, where robust negative selection is observed. We demonstrate here that PD-1
+
CD4
+
peripheral (Tph) helper-like and follicular (Tfh) helper-like T cells developing in HIS mice can induce autoimmune disease. Tfh-like cells were more prominent in HIS mice with a mouse thymus, in which the highest levels of IgG were detected in plasma, compared to those with a human thymus. While circulating IgG and IgM antibodies were autoreactive to multiple mouse antigens, in vivo depletion of B cells and antibodies did not delay the development of autoimmune disease. Conversely, adoptive transfer of enriched Tfh- or Tph-like cells induced disease and autoimmunity-associated B cell phenotypes in recipient mice containing autologous human APCs without T cells. T cells from mice with a human thymus expanded and induced disease more rapidly than those originating in a murine thymus, implicating HLA-restricted T cell-APC interactions in this process. Since Tfh, Tph, autoantibodies and LIP have all been implicated in various forms of human autoimmune disease, the observations here provide a platform for the further dissection of human autoimmune disease mechanisms and therapies.
Enhancing Student Engagement in Entrepreneurship Education for Undergraduate Student through Gamification Methods
Authors: Satria Vinza Hedi , Zuhroh Nur Fatimatuz , Sari Juwita
Published: July 2024

Aims: This research aimed to assess and implement gamification methods in teaching entrepreneurship learning materials at the undergraduate level. The study anticipates its findings to serve as a benchmark for educators seeking to enhance student engagement in their courses, particularly in Entrepreneurship. These outcomes are expected to offer practical insights and strategies for improving learning experiences and outcomes across various educational disciplines.
Study design:  The research’s method consists of three phases: First is to design the gamification method, second is the implementation and third is evaluation to understand the effect of gamification on student engagement in class.
Place and Duration of Study: The research will be conducted over a period of one week for a single topic. It will involve one class of college students which consists of 48 students from those who are enrolled in the entrepreneurship course.
Methodology: The first phase of the research will design the gamification method for one learning material. For current research, one learning material were chosen based on curriculum applied by Sekolah Tinggi Ilmu Ekonomi Indonesia Surabaya. The curriculum from mentioned educational institute were chosen since all the respondents participated in the research were student from fore-mentioned educational institute. Thus, a gamification of Break-Even Point was created during this phase. Second, the implementation, the students were asked to do certain assignments related to the research. Third, after completing the assignments, students are asked to provide evaluations of the tasks they worked on in the form of questionnaire surveys. These evaluations are then analyzed and form the basis of the research results.
Results: From 48 students, roughly 88% of the student (42 students) were interested with the gamification method given to them and it increase their knowledge on the topics. 10 students already know the topic delivered by the gamification from previous course they had attended but think that the gamification method is interesting. The rest of the students feel no effect from the gamification at all.
Conclusion: From the result of the research, it is safe to assume that gamification method is effective to increase student engagement. Most students agreed when asked if the Entrepreneurship material presented was enjoyable. When asked if the method used was more engaging than traditional oral teaching, most students also agreed. Some students even suggested using the same method for future lessons.
Physiological and Biochemical Basis of Thermotolerance in Selectively Fertilized Coconut Hybrids
Authors: N. A. Aisha Renju , Stephen Roy , Manju R. V. , M. Viji M.
Published: July 2024

The aim of the study was to physiologically and biochemically assess selectively fertilized coconut hybrids for their tolerance to high-temperature stress. Conducted using a two-factorial Completely Randomized Design, the research took place at the Department of Plant Physiology, College of Agriculture, Vellayani, Trivandrum, between October 28, 2023, and December 28, 2023. Methodology involved cultivating six-month-old selectively fertilized hybrid seedlings of three coconut varieties, Kerasree, Keraganga, and Kerasankara, in a polyhouse with a pre-set temperature of 40±1°C for two months. Various physiological and biochemical parameters, including Relative Water Content, Chlorophyll Stability Index, Epicuticular Wax Content, Lipid Peroxidation Level, and antioxidant enzyme activity, were measured to assess stress tolerance. Results showed that for the physiological parameters RWC and CSI, selectively fertilized hybrids varied significantly under high temperatures from their corresponding non-fertilized hybrids, with average mean values of 88.53% and 83.27%, respectively. High-temperature-induced solute leakage was lowest in Kerasankara selectively fertilized hybrids with a mean value of 3.58±0.63, and wax deposition values were on par for selectively fertilized and non-fertilized hybrids. MDA accumulation due to lipid peroxidation was significantly lowest in selectively fertilized hybrids with a mean value of 2.47µg g-1. Antioxidant enzymatic activity in selectively fertilized hybrids was remarkably higher, with significant percent increases of 97.1% for Peroxidase and 96.1% for SOD in the Keraganga selectively fertilized genotype. The study concluded that coconut hybrid accessions developed through pollen selection demonstrated significant superiority over regular hybrids in terms of physiological and biochemical stress tolerance indices. Thus, pollen selection followed by selective fertilization is a reliable, cost-effective, and time-saving approach for breeding stress-tolerant crops, including perennials.
Long Term Effect of Organic and Inorganic Sources of Nutrient on Growth of Pearl Millet (Pennisetum glaucum)
Authors: Yadav Indra Raj , Trivedi S. K. , Yadav Shashi S. , Sharma Amita , Khambalkar Priyadarshani A. , Gurjar N. S.
Published: July 2024

In today’s era the farmers using traditional fertilizers in very high amount, so the soil fertility and productivity decreasing day by day. In this context the present study was planned using organic and inorganic sources of fertilizers with their long-term impact on soil fertility, productivity and soil heath. The present study carried out at Research Farm, department of Soil Science, Rajmata Vijayaraje Scindia Krishi Vishwavidyalaya, Gwalior during kharif season 2022 and 2023. The twelve treatments combinations of organic and inorganic sources were used for the existing study. The data revealed that the higher pooled mean plant height (188.79 cm), length of cob (31.88 cm), dry matter accumulation (44.90 g) and grain weight per cob (10.87 g) was registered under T12-100% NPK +FYM @ 10 t/ha/yr + {PSB+ Azotobacter (Seed treatment)}. It was concluded that treatment 100% NPK +FYM @ 10 t/ha/yr + {PSB+ Azotobacter (Seed treatment)} was found superior among remaining treatment.
Assessment of Drought Stress of Two Genotypes of Sorghum (Sorghun bicolor L.) Using Physiological Parameters
Authors: Tambe Snehal A. , A. S Jondhale, , Wagh B. D.
Published: July 2024

The present experiment conducted in the period of rabi season at Sorghum Improvement Project, M.P.K.V., Rahuri, Maharashtra during the period of 2016 to 2017. During investigation to observed the physiological parameters under non stress and moisture stress condition with cross two genotypes SPV 1830 x IS 6427. In this study of mean performance of the basic generation P1, P2, F1, F2, B1 and B2 of the cross SPV 1830 x IS 6427 was observed various physiological parameters viz. photosynthetic rate, rate of transpiration, stomatal conductance, relative leaf water content, SPAD reading, total chlorophyll content and chlorophyll stability index. The present results to observed the B2 and F1 generation recorded significantly highest photosynthesis rate (25.98 and 22.47 µ mole CO2 m-2s-1) under non stress and moisture stress condition, respectively. The parent B1 recorded highest transpiration rate (3.27 and 1.92 mmole H2O m-2s-1) under non stress and moisture stress condition, respectively. The F1 and B1 generation recorded significantly the highest mean for stomatal conductance (0.24 and 0.18 mole m-2 s-1) under non stress condition and moisture stress condition, respectively. The B1 generation recorded the highest total chlorophyll content (3.30 and 2.08 %) under non stress and moisture stress condition, respectively. The present experimental study to observe physiological parameters of sorghum cross two genotype (SPV 1830 x IS 6427)   directly affected in growth and grain yield under non-stress and moisture stress condition. Therefore, present study to find out the drought stress effects on sorghum through the physiological parameters.
Attitude and Practice of Female Genital Mutilation among Women of Reproductive Age in a Tertiary Health Facility in Benin City, Edo State
Authors: Saka Sulymon A. , Mavis Eromosele E. , Meshach Osejele I. , Jeremiah Okpu U. , Hendrix Odion E. , Godson Obokhai O. , Elochukwu Chukwuka E. , Christabel Ukpetenan I. , Courage Williams I. , Emekolom Obiajulum N.
Published: July 2024

Background: Female genital Mutilation (FGM) is a harmful traditional practice prevalent in Africa, leading to adverse physical, psychological, sexual, and reproductive health outcomes for women.
Objective: This study aims to ascertain the attitude and practice of female genital mutilation (FGM), the extent of FGM practice, and the determinant factors influencing FGM among women of reproductive age attending tertiary health facilities in Benin City, Edo State.
Methodology: A cross-sectional descriptive study design was used. A sample of 316 women was selected using a simple random sampling technique. A self-structured questionnaire served as the instrument for data collection. Data analysis was conducted using the Statistical Package for Social Sciences (SPSS).
Results: The study revealed that 54.1% of respondents have undergone FGM, while 45.9% have not. A significant portion of respondents (48.4%) believe that FGM aims to ensure premarital virginity and marital fertility. Moreover, 65.2% of respondents indicated that FGM is performed in unclean environments.
Recommendation: It is recommended that healthcare workers engage in health education about the health, sexual, and psychological consequences of FGM to mitigate its practice.
Canopy Management in Guava with Inorganic and Organic Fertilizer is a Boon for the Farming Community for its Continued Yield
Authors: Kumari Mamta , Kumar Rajesh
Published: July 2024

Guava fruit is known for its delicacy having Balance acid-base relationship, rich in minerals, salt, and vitamins. In other words, it is a poor man’s apple. Krishi Vigyan Kendra Sabour Bhagalpur has established a guava orchard of 1.75 5. hectares of select and selected Allahabad safeda variety for trial in training and pruning of guava and its management in other parts of the year during 2015-16,16-17&17 -18. As per recommendation, Guava, canopy management requires three training and pruning. In KVK trial we have gone through two training pruning one deep pruning in end of January & Feburary with fertiliser and insect disease management, and in September-October, light pruning with the recommended dose of fertiliser that is 100g urea + 75g DAP + 150g NOP + 2.5kg Compost+ 250 g Azospirrilium @ 30 days of interval has shown the best result average   yield 12 kg fruits/plants from four-year-old plants in rainy season harvest. Planting distance was kept at 2m x 3m I.e. plant to plant distance 2. And Row to row distance 3m that accommodate 1667 plants in one hectare of land yields 200 quintal sold at 15/kg.  Light training and pruning was followed with fertilizer application yields 4.5 kg fruits per plant.  Total yield obtained was 75 quintal sold @ rs 70 /kg with successive flowering that gives extra yield to the farmers at high remuneration and disease free production.
Development of Hybrids with Promising Restorers under Test Cross Nursery Study in Rice (Oryza Sativa L.)
Authors: Madhukar P. , Srinivas B. , Nayak P. Gonya , Prasanna B. Laxmi
Published: July 2024

The experiment was carried out during Rabi, 2018-19 and thirty-four rice stable lines crossed with five (5) wild abortive cytoplasmic male sterile (CMS) line to develop 170 F1s. Those 170 F1s were evaluated in test cross nursery evaluation (TCN) during Kharif, 2019 along with two locally popular checks Jagtiala Rice-1 (JGL 24423) and Bathukamma (JGL 18047). Among those 170 F1s, 32 restorer cross combinations, 23 maintainer cross combinations and remaining 114 partial cross combinations (partial restorers or partial maintainers) were identified based on pollen fertility (%) and spikelet fertility (%). In restorer cross combinations, four stable lines viz., JGL 36147, JGL 37228, JGL 36181 and JGL 37207 having spikelet fertility %, Pollen fertility% (90.5%,89.7%), (90.5%, 93%), (95%, 85%) and (90.6%, 89%) recorded respectively. In maintainer cross combinations, three stable lines JGL 36181, VT-101 and VT-106 recorded 0 to 1 % spikelet and pollen fertility percentage. Among the 32 restorer cross combinations, best 14 cross combinations (based on yield and its attributing characters) were promoted to preliminary yield trial (PHT) and evaluated in Kharif, 2020. Among the 14 hybrids, two hybrids namely, JGLH 447 (5.9 t/ha, 16.5%) and JGLH 442 (5.3 t/ha, 14.6%) recorded significant higher yields and low percent of gall midge incidence over locally cultivated cultivars, JGL 24423 (4.4 t/ha, 16.8%), US 312 (4.3 t/ha, 36.2%), 27P31 (4.2 t/ha, 35.6%) and HRI 174 (3.2 t/ha, 37.1%).
Understanding Future Healthcare Providers' Knowledge, Attitudes and Practices on Supplement Use at a Private Medical College in Lahore
Authors: Maqsood Ghanwa , Qasim Muhammad , Arshad Memoona , Ali Abu Sufiyan , Shah Ahmad Hanan , Shakoor Afshan Abdul , Latif Shaikh Muhammad Usama , Iqbal Muhammad Zahid
Published: July 2024

Background: The use of dietary supplements without healthcare provider recommendations is widespread, although many supplements should ideally be used under professional supervision.
Objective: This study aimed to evaluate the knowledge, attitude, and perception of future healthcare providers regarding the use of supplements.
Methods: A validated questionnaire was administered to future healthcare providers from various faculties, including medical, dentistry, and pharmacy. The questionnaire was divided into segments: demographics, attitude, knowledge, and perception regarding the use of supplements.
Results: The study revealed that female students exhibited a more positive attitude towards the use of supplements, with a mean score of 44.43 out of 50 (SD = 2.11), compared to males who scored 39.32 (SD = 4.65). Final-year students had a better attitude compared to pre-final-year students (P = 0.001, η² = 0.213). In terms of knowledge, females scored higher, averaging 8.9 out of 10. Pharmacy students demonstrated better knowledge compared to students from other faculties (P = 0.35, η² = 0.14). Perception results indicated that females had a more positive perception than males, and non-hostellers had a better perception compared to hostellers (P = 0.008, η² = 0.18). Overall, females, pharmacy students, and final-year students showed better knowledge, attitude, and practices regarding supplement use.
Conclusion: The study highlights the importance of targeted educational interventions to improve the knowledge, attitude, and perception of future healthcare providers regarding the appropriate use of supplements, with particular attention to gender and faculty differences.
Animal Models for Candidiasis
Authors: Conti Heather R. , Huppler Anna R. , Whibley Natasha , Gaffen Sarah L.
Published: April 2014

Multiple forms of candidiasis are clinically important in humans. Established murine models of disseminated, oropharyngeal, vaginal, and cutaneous candidiasis caused by Candida albicans are described in this unit. Detailed materials and methods for C. albicans growth and detection are also described. Curr. Protoc. Immunol. 105:19.6.1‐19.6.17. © 2014 by John Wiley & Sons, Inc.