bluuebunny/crossref_metadata_2025 huggingface dataset:
A novel insight on SARS-CoV-2 S-derived fragments in the control of the host immunity
Authors: Bastos Thais Sibioni Berti , de Paula André Guilherme Portela , Luz Rebeca Bosso dos Santos , Garnique Anali , Belo Marco , Eto Silas , Fernandes Dayanne , Ferraris Fausto , De Pontes Leticia , Franca Tabata , Barcellos Leonardo , Veras Flavio , Bermejo Pamela , Guidelli Giovanna , Maneira Carla , Mello Fellipe , Teixeira Gleidson , Pereira Gonçalo , Fernandes Bianca , Galindo-Villegas Jorge , Sanches Paulo Ricardo , Braz Helyson , Jorge Roberta , Malafaia Guilherme , Cilli Eduardo , Olivier Danilo , Amaral Marcos , Medeiros Renata , Condino-Neto Antonio , Carvalho Luciani , Machado-Santelli Glaucia , charlie-silva Ives , Braga Tarcio , Souza Giovane
Published: October 2022

Despite all efforts to combat the pandemic of COVID-19, we are still living with high numbers of infected persons, an overburdened health care system, and the lack of an effective and definitive treatment. Understanding the pathophysiology of the disease is crucial for the development of new technologies and therapies for the best clinical management of patients. Since the manipulation of the whole virus requires a structure with an adequate level of biosafety, the development of alternative technologies, such as the synthesis of peptides from viral proteins, is a possible solution to circumvent this problem. In addition, the use and validation of animal models is of extreme importance to screen new drugs and to compress the organism's response to the disease. Peptides derived from recombinant S protein from SARS-CoV-2 were synthesized and validated by in silico, in vitro and in vivo methodologies. Macrophages and neutrophils were challenged with the peptides and the production of inflammatory mediators and activation profile were evaluated. These peptides were also inoculated into the swim bladder of transgenic zebrafish larvae at 6 days post fertilization to mimic the inflammatory process triggered by the virus, which was evaluated by confocal microscopy. In addition, toxicity and oxidative stress assays were also developed. In silico and molecular dynamics assays revealed that the peptides bind to the ACE2 receptor stably and interact with receptors and adhesion molecules, such as MHC and TCR, from humans and zebrafish. Macrophages stimulated with one of the peptides showed increased production of NO, TNF-α and CXCL2. Inoculation of the peptides in zebrafish larvae triggered an inflammatory process marked by macrophage recruitment and increased mortality, as well as histopathological changes, similarly to what is observed in individuals with COVID-19. The use of peptides is a valuable alternative for the study of host immune response in the context of COVID-19. The use of zebrafish as an animal model also proved to be appropriate and effective in evaluating the inflammatory process, comparable to humans.
Novel magnetic graphoxide/biochar composite derived from tea for multiple SAs and QNs antibiotics removal in water
Authors: Pang Wei , Wang Yonghui , Li Shuang , Luo Yuanyuan , Wang Guanyu , Hou Jian , Han Tie , Gao Zhixian , Guo Qingbin , Zhou Huanying
Published: October 2022

Increasing antibiotics pollution is becoming an urgent public health issue. Biochar is a kind of promising solution for antibiotic removal in aqueous environment. However, the adsorption and degradation performance of pristine biochar was limited because of the relatively lower specific surface. The remediation efficiencies could be highly improved through developing new biochar-based composites which have high interactions and bonding abilities with antibiotic. In this study, a novel magnetic graphoxide/biochar composite (mGO/TBC) was fabricated as an efficient and recyclable persulfate (PS) activator for degradation and removal of sulfonamides (SAs) and quinolones (QNs) antibiotics. Based on the synergism presorption and degradation of graphoxide and biochar, the removal rates of mGO/TBC for sarafloxacin hydrochloride, sulfadimethoxine, sulfapyridine, sulfado xine, sulfamonomethoxine, sulfachloropyridazine, enrofloxacin, and ciprofloxacin were increased above 85%. Quenching experiment and ESR analysis had proved that 1O2, •OH and SO4•− from mGO/TBC/PS system were the primary oxidation active species to degrade SAs and QNs. Furthermore, the mGO/TBC is easy to be batch produced due to simple synthesis steps. It is a promising substrate for antibiotic bioremediation with good application prospects.
Evaluating the Benefits of a High-Fidelity Simulation Workshop for Head and Neck Examination Skills
Authors: White Jessica , Mattingly Laura , Johnston Elizabeth , Sengupta Samarpita
Published: October 2022

Background
The COVID-19 pandemic has presented unique challenges for health professions education particularly in the area of teaching physical examination skills. As safety restrictions have limited close physical contact, programs have had to utilize alternate teaching methods to ensure students can practice and develop necessary skills. Accordingly, the UT Southwestern Masters in Physician Assistant Studies program developed a high-fidelity simulation workshop using simulation mannequins and task trainers to allow students to practice, under faculty guidance, components of the head and neck examination.
Methods
We collected quantitative and qualitative data using surveys to determine the impact of this innovative teaching tool on student confidence with performing the head and neck examination. In addition, experienced faculty members evaluated student competency using traditional rubrics.
Results
We found significant improvements in self-reported confidence and skill in the otoscopic and ophthalmic examinations individually as well as in the comprehensive head and neck examination. These self-reported improvements also correlated with scores on faculty-graded examinations.
Conclusions
Our findings impact not only the confidence and proficiency of examination skills in this student cohort, but have the potential to improve future health professions education.
Comparing the Effectiveness of Two Surgical Techniques for Treating Lower Lid Epiblepharon in Children: A Randomized Controlled Trial
Authors: Takeuchi Masaki , Matsumura Nozomi , Ohno Tomoko , Fujita Takeshi , Asano Mizuki , Mizuki Nobuhisa
Published: October 2022

A multicenter randomized controlled trial was conducted to compare the effectiveness of incisional and nonincisional surgical techniques for treating lower lid epiblepharon in children. The study included 89 eyes from 50 children aged 3–15 years (mean, 7.5 ± 2.4 years) with clinically significant moderate lower lid epiblepharon. Patients were randomly assigned to either incisional (modified Hotz procedure with lid margin splitting; 45 eyes of 25 patients) or nonincisional (44 eyes of 25 patients) surgery groups. Treatment outcomes were evaluated 6 months after surgery and classified into three grades: well-corrected, no ciliary touch, and superficial keratitis; under-corrected, ciliary touch, and superficial keratitis; and over-corrected, eyelid ectropion, and insufficient eyelid closure. Changes in astigmatism were compared between the two techniques after surgery. Incisional surgery provided a significantly higher percentage (77.8%) of well-corrected treatment results (P = 0.026; odds ratio, 2.88; 95% confidence interval, 1.07–8.22) than nonincisional surgery (55.4%). The mean change in astigmatism pre and post surgery was − 0.24 ± 0.42 and − 0.01 ± 0.47 D in the incisional and nonincisional surgery groups, respectively. The improvement in astigmatism 6 months after surgery was significantly higher in the incisional surgery group than in the nonincisional surgery group (P = 0.008). The incisional surgical treatment for epiblepharon in children resulted in a higher number of well-corrected patients, indicating an absence of both ciliary touch and superficial keratitis as well as statistically significant improvements in astigmatism correction.
Study on the correlation between phase angle and nutritional status and sarcopenia in patients with cancer pain
Authors: Chen Yajun , Zhang Ping , Zeng Yaqi , Li Chunlei , Dong Jie , Li Yueying , Wang Yujie , Liu Heji , Wang Kun
Published: October 2022

Objective
To observe the relationship between PA measured using the bioelectrical impedance method and nutritional risk, nutritional status, and sarcopenia in patients with cancer pain.
Methods
146 patients with cancer pain were enrolled.NRS, NRS-2002, PG-SGA, anthropometrics, blood biochemical examination, body composition analysis and other methods were performed for pain assessment, nutritional risk screening, and nutritional status assessment. Patients were divided according to PA. For the low PA and the normal PA groups, the correlation between PA and pain score, nutritional risk,and nutritional indicators was analyzed.
Results
TP,ALB,CHE,HB,PALB,and HGS in the low PA group were lower than those in the normal PA group (P < 0.05);the low PA group had higher NRS,NRS-2002,and PG-SGA scores than the normal PA group (P < 0.05); ICW, ECW, PRO, FM, FFM, ASMM, BCM, ASMI in the low PA group were significantly lower than those in the normal PA group (P < 0.05);PA and HGS, PALB, ALB,TP,ICW,PRO,FFM,ASM,BCM,ASMI were positively correlated (P < 0.05);PA was negatively correlated with pain score, nutritional risk, and PG-SGA score (P < 0.05).
Conclusion
PA is an economical and effective objective indicator that reflects the nutritional status of patients with cancer pain and is closely related to the degree of pain, nutritional status, and muscle loss in patients. The detection of the phase angle helps to evaluate the nutritional status of patients with
Identification of potential ferroptosis hub genes in acute-on-chronic liver failure based on bioinformatics analysis and experimental verification
Authors: Kuang Meixia , Cai Longhui , Zhao Jing , Huang Liqiao , Ji Yichun , Lv Bingyao , Kuang Weihong
Published: October 2022

Background
Ferroptosis has an important role in developing Acute-on-chronic Liver Failure (ACLF). The present project aimed to identify and validate the potential ferroptosis-related genes in ACLF by bioinformatics analysis and experimental verification.
Materials and Methods
GSE139602 was obtained from the GEO dataset and intersected with ferroptosis genes. Ferroptosis-related differentially expressed genes (DEGs) between the ACLF tissue and healthy group were analyzed using bioinformatics methods. Analysis of enrichment, protein-protein interactions, and hub genes were conducted. Finally, we performed real-time quantitative PCR(RT-qPCR) to validate the expression of the hub genes.
Results
A total of 35 ferroptosis-related DEGs were screened, which were involved in biosynthesis of amino acids, peroxisome, and fluid shear stress and atherosclerosis. PPI network analysis indicated five ferroptosis-related hub genes namely HRAS, TXNRD1, NQO1, PSAT1, and SQSTM1. The experimental validation indicates that the expression levels of HRAS, TXNRD1, NQO1, and SQSTM1 were lower, while PSAT1 was higher in ACLF rats than that of healthy group.
Conclusions
Our findings reveal that PSAT1, TXNRD1, HRAS, SQSTM1 and NQO1 may affect the development of ACLF by regulating ferroptotic events. These results provide a valid reference for potential mechanisms and identification in ACLF.
The Reality Check of Community Clinic in Bangladesh: A Case Study of Two Selected Villages of Naogaon District
Authors: BakiBillah Abul Hasan
Published: October 2022

Community Clinic (CC) as a new horizon in ensuring primary healthcare in Bangladesh has been working astoundingly since its inception. Reportedly, some of the CCs are not working effectively that’s why the beneficiaries, the villagers, are seeking care from other professionals. To address the underlying reasons of ineffectiveness of the CC in the site was the key objective of this study. Lack of work in that particular context motivates to pursue this study. Due to the financial constraint, the sampling site, Naogaon district, was purposively selected. Then the randomly selected upazila, Raninagar, was undertaken. Applying multi-stage random sampling technique, primary data of 100 household of two villages, as per the secondary data, were collected. From the empirical investigation, it is addressed that the key determinants of demand for health care from the CC; the level of education and level of income; are not playing a robust role. Instead of those, the problem of comparative low use rate of the CC lies on the determinants of supply-side; the unavailability of the CHCP and the drugs, which ultimately strengthens the traditional outlook towards the CC by the villagers. Once the provision of drugs is ended, the CC becomes literally meaningless except few responsibilities conducted by the health workers. Along with the government’s strong commitment, some reforms; formation of Village Solidarity Group’ keeping educational institute at the centre; can transform the scenario drastically.
Burnout prevalence and its associated factors among Chinese webcasters
Authors: Wang Hanqin , Yang Shang , Zhang Fushen , Gao Xiao , Chen Shi , Liu Ziwei
Published: October 2022

Background
Webcaster is a young and high-income job, with a low barrier to entry, attracting a large number of people entering, however, huge job stress, job-related health problems, lack of labour and social security are leading to a wide unhealthy employee turnover. Data on the prevalence of burnout in webcaster are very limited. This study aims to investigate the prevalence and correlates of burnout among Chinese webcaster.
Methods
A total of 358 webcasters were recruited from 4 companies in Changsha, China. Socio-demographic information, career-related factors, job stress, interpersonal support, and burnout were collected by a self-design questionnaire through online platform.
Results
Nearly half (46.09%, 95% CI: 40.90 ~ 51.02%) of the webcasters met the high burnout criteria. In multiple logistic regression analysis, risk factors associated with burnout include lower education (junior high school and below: OR = 9.87), cyber violence (OR = 6.79), losing fans (OR = 4.37), longer live broadcast duration (> 8h per day: OR = 3.57), negative performance appraisal (OR = 2.61), longer resting (OR = 2.56), low income (OR = 2.41), live broadcasting time at day (OR = 2.30), higher seniority (OR = 2.34), job stress (OR = 1.19). The protective factor is interpersonal support (OR = 0.83).
Conclusions
Burnout is prevalent in Chinese webcasters. Dynamic risk factors include losing fans, longer live broadcast duration, negative performance appraisal, low income and daily live broadcast time, as well as job stress. Static risk factors include lower education, cyber violence, and higher seniority. The protective factor is interpersonal support. these findings may contribute to the risk management and health promotion of webcaster’s burnout.
MicroRNAs 7/17/155 As A Potential Breast Tumor Stemness miRNA Cluster: Detection of Breast Cancer Grade Specific miRNA-mRNA Interaction Network Inspiration from Mammary Gland Development
Authors: Khodayari Saeed , Khodayari Hamid , Jallali Haniyeh , Saeedi Elnaz , Faryabi Ali , Yeganeh Meghdad , Mohammadnejad Ahad , Razavi Amirnader Emami , Dashtkoohi Mohammad , Hadjilooei Farimah , Shirkoohi Reza , Omranipour Ramesh , Nayernia Karim , Mahmoodzadeh Habibollah
Published: October 2022

Background The process of breast tumor dedifferentiation is complex and unclear. The mechanism represents the origin of the genesis and development of high-grade breast stem cells. It seems that microRNAs have crucial regulatory functions in this complicated phenomenon. The main objective of this study is to identify a potential "breast tumor stemness miRNA cluster" using an in silico strategy and qRT-PCR validation guided by the molecular pattern of mammary gland development (MGD). Methods Microarray databases GEO and ArrayExpress were used to determine mRNA and microRNA expression in different grades of breast carcinoma (BC). Differential gene expression of mRNA (GSE29044) and miRNA (GSE4566) in three grades of BC was analyzed using GEO2R compared with normal tissue. The enrichment results revealed MGD -associated mechanisms and target mRNAs. Using the BC database, the interaction between target mRNAs and significantly altered miRNAs (PV ≤ 0.05) in each BC grade was found by miRNet. After confirming our results using the GSE26659 data, the expression of the target miRNAs in tissue samples (24 BC, 17 normal tissues) was examined by real-time PCR. miRwalk and the STRING database discovered the miRNAs of interest and mRNA networks. Results The MGD stages of puberty, pregnancy and lactation, and mammary gland epithelial development were significantly involved in the upregulated genes of GI and GII tumors. No significant upregulated MGD mechanisms were detected in GIII BC. In silico analysis revealed that miRs 7/17/155 had an upregulation pattern and miR-26a had a downregulation pattern. qPCR showed that the miRNAs 7/17/155 were significantly upregulated in GIII tumors (PV ≤ 0.05), while there were no notable changes in miR-26a. EGFR was the central node regulated by the miR 7/17/155 intermediate mRNA cluster. Conclusions Our results suggest that microRNAs 7/17/155 may be a potential cluster associated with formation of breast tumor stemness. This cluster can be used for the breast cancer dedifferentiation therapy or molecular classification of mammary tumor differentiation status.
Integrating External Factors and Technology Acceptance Model to Understand Scholar Intention and Use of Recommendation System for Course Selection.
Authors: Zameer Zameer Gulzar , Fathima Fatima Amer Jid Almahri , Afrah Afrah Fathima
Published: October 2022

There has been a huge increase in e-learning courses, and the Recommendation systems (RSs) are useful tools for narrowing down the course options and expose students to the courses that suit their needs. The majority of the research related to recommendation systems focus on effectiveness rather than factors influencing its acceptability, and in practice, user satisfaction cannot be explained by accuracy alone. This study will consider course recommendation system and investigate the acceptance of the courses suggested by the recommender system (RS) to scholar’s or research students based on their learning needs which in turn will help to find out why some individuals accept new technology while others resist. Therefore, research scholars (n = 150) who willingly engaged in this study were asked to use the RS and complete a questionnaire also based on their experience as part of a self-administrated longitudinal survey. This study assessed external factors such as perceived availability, relevance, and experience, which were not explained by the Technology Acceptance Model (TAM). The results confirm that the extended TAM provides a valuable theoretical model, which helps to understand scholar’s acceptance of RS, and other factors positively that affect the original variables of TAM. Hence, a new modified TAM that incorporates three external factors has been offered. The considerable value calculated for Cronbach’s alpha confirms that the results are valid and reliable. The observed results will help recommendation system developers to maximize user experience because the implications of this research effort are critical for instructors, scholar’s, and institutions as well.
Variation in the local grey mullet populations (Mugil cephalus) on the western Pacific fringe
Authors: Kuo Chien-Hsien , Lee Sin-Che , Du Shin-Yi , Huang Chao-Shen , Lin Hung-Du
Published: October 2022

Understanding population genetic structures is crucial for planning and implementing conservation programmes to preserve species’ adaptive and evolutionary potential and thus ensure their long-term persistence. The grey mullet (Mugil cephalus) isa globally distributed coastal fish. Its populations in waters surrounding Taiwan on the western Pacific fringe are divided into at least two stocks (migratory and residential), but questions remain regarding their genetic divergence and gene flow. To cast more light on this, allozyme variations at 21 presumptive gene loci of 1,217 adult grey mullets from 15 localities in Japan, Taiwan and mainland China, and four gene loci from 1,470 juveniles from three localities in Taiwan were used to investigate patterns of genetic variation. The mean expected heterozygosity (He) was 0.128—ranging from 0.031 (Matsu) to 0.442 (Kaoping)—and the mean observed heterozygosity (Ho) was 0.086—ranging from 0.017 (Kaohsiung) to 0.215 (Kaoping). Both AMOVA and the high overall meanFSTof 0.252 indicated enormous genetic differentiation among populations and the positive mean value of FISwas 0.328, indicating a deficiency of heterozygotes. PCA analysis indicated that the samples ofM. cephaluscould be split into four groups and STRUCTURE analysis showed that all individuals were grouped into three genetic clusters. Results of mutation-drift equilibrium tests did not suggest that the populations experienced any recent genetic bottleneck. Results from all localities in the present investigation showed that significant change in the GPI-A genotype frequencies with latitudes—e.g., an increase inGPI-A * 135/135homozygote frequencies andGPI- A * 100/100frequencies were highly correlated with latitudinal cline. T All migratory populations with the GPI-A genotype were almost exclusively theGPI-A * 100/100homozygote. During the life history ofM. cephalus, theGPI-A * 100/135heterozygote frequency significantly decreases with age. Based on these data, we suggested that each GPI-A genotype represents trait combinations of higher fitness in some portions of the environment. Furthermore, the genotypic frequencies change in accordance with life stages, suggesting that selection occurs throughout the life span.
Predictors of cancer rehabilitation medicine referral and utilization among cancer survivors
Authors: Brick Rachelle , Natori Akina , Moreno Patricia , Molinares Diana , Koru-Sengul Tulay , Penedo Frank
Published: October 2022

Purpose
Cancer survivors experience high rates of physical inactivity that often go unaddressed. The My Wellness Check program (MWC) is an EHR-integrated screening and referral system that includes surveillance of physical activity and triage to cancer rehabilitation medicine services. This study examined assessment of physical activity and subsequent referrals to cancer rehabilitation medicine.
Methods
A secondary analysis was performed for survivors who completed the MWC between April 2021 and January 2022. Univariable and multivariable logistic regression modeled determinants of qualification for a physical activity referral and provider completion of referral to cancer rehabilitation medicine. Referral was based on the Moving through Cancer questionnaire. Adjusted odds ratios (aOR) and corresponding 95% confidence intervals (95% CI) were calculated.
Results
There were 1,174 survivors who completed the physical activity survey, of which 46% (n = 540) qualified for a physical activity referral. After controlling for group differences, qualification for a referral included moderate-severe physical dysfunction (aOR: 1.815; 95% CI: 1.177, 2.798), and Hispanic or Latino ethnicity (aOR: 0.744; CI: 0.574, 0.963). Only 31% (n = 168) received a completed physician referral to cancer rehabilitation medicine following screening. No patient-level factors were significantly associated with receiving a completed physician referral. Following referral, 8% (n = 13) utilized cancer rehabilitation medicine services.
Conclusions
Patient-level and clinical factors may predict qualification for physical activity referrals; however, they don’t appear to predict referral completion to cancer rehabilitation medicine services. Future research should focus on potential provider- and organization-level factors that interact and influence referral to and utilization of cancer rehabilitation medicine services.
Serum Klotho and Cognitive Functioning in Older Adults Aged 60 and 79: The National Health and Nutrition Examination Survey 2011 to 2014
Authors: Ge Song , Dong Fanghong , Xu Li , Tian Chong , Yang Chih-Hsiang , Wei Jingkai
Published: October 2022

Objectives
Klotho is found to be associated with better cognitive outcomes in selected small sample of aged population. We aimed to examine the association of serum klotho with cognitive function among older adults using a nationally representative sample of the U.S.
Method
A total of 2,173 older adults aged 60–79 years in the National Health and Nutrition Examination Survey from 2011 to 2014 were included. Klotho was measured and quantified in laboratory. Cognitive function was measured using the Consortium to Establish a Registry for Alzheimer’s Disease Word Learning subtest [CERAD-WL] immediate and delayed memory, the Animal Fluency test [AFT], the Digit Symbol Substitution Test [DSST]). Test-specific and global cognition z-scores were calculated based on means and standard deviations. Multivariable linear regression models were applied to examine the association of quartiles of serum klotho with test-specific and global cognition z-scores. Subgroup analysis was conducted by sex.
Results
Serum klotho level in the lowest quartile was associated with lower z-score for DSST (beta (β)=-0.13, 95% confidence interval (CI): -0.25, -0.01). For subgroup analysis, serum klotho level in the lowest quartile was associated with lower z-scores for DSST (β=-0.16, 95% CI: -0.32, -0.003) and global cognition (β=-0.14, 95% CI: -0.28, -0.01) among women. No significant association was found among men.
Conclusions
Low serum klotho was associated with poorer cognitive function, particularly among women. Future studies are expected to examine the longitudinal associations between klotho levels and cognitive outcomes.
Probabilistic Seismic Risk Assessment Framework: Case study Adapazari, Turkey
Authors: Sianko Ilya , Ozdemir Zuhal , Hajirasouliha Iman , Pilakoutas Kypros
Published: October 2022

While earthquakes can have a devastating impact on the economic growth and social welfare of earthquake prone regions, probabilistic seismic risk assessment can be employed to assess and mitigate such risks from future destructive events. In a previous study (Sianko, 2020), a probabilistic seismic hazard analysis (PSHA) tool based on the Monte-Carlo (MC) approach, was developed to predict the seismic hazard for high seismicity areas. In this study, a seismic risk assessment framework is developed incorporating the previously developed PSHA tool, with vulnerability functions based on various damage criteria, exposures and casualty models. Epistemic uncertainty is addressed using logic trees and distribution functions. The developed seismic risk assessment framework can estimate human and economic losses for particular return periods using an event-based stochastic procedure. The framework is applied to a case study area, the city of Adapazari in Turkey. Seismic risk assessment is carried out for different return periods to identify the most vulnerable areas of the city. The verification of the developed seismic risk framework is performed by comparing the predicted seismic losses to those observed during the 1999 Kocaeli earthquake that severely affected the city of Adapazari. The results of the study indicate that while overall predictions for extensive and complete damage states demonstrate strong correlation with the observed data, accurate risk predictions at the district level are not achievable without microzonation studies.
Perception and usage of short-term prednisone and prednisolone in dogs
Authors: Gober Margaret , Hillier Andrew
Published: October 2022

Background: Corticosteroids are widely used with a broad level of comfort in the hands of most veterinarians. With this level of comfort there may be complacency and underestimation of the impact the side effects of corticosteroids may have on a pet and pet owner.
Objective: The objective of this clinical study was to understand the experience and perceptions of an owner using anti-inflammatory doses of oral prednisolone and prednisone for up to 14 days. We hypothesized dogs receiving anti-inflammatory doses of prednisone and prednisolone would experience much greater rates of side effects by day 14 then reported in current literature.
Animals: There were 45 dogs initially enrolled in the study, with 33 dogs completing the study.
Results: At each study point, 31 owners provided results. On day 5, 74% (23/31) reported at least 1 change in their dog’s behavior including polyuria, polydipsia, polyphagia, polypnea and/or increased vocalization, with 11 individuals (35%) reporting these changes greatly increased. On day 14, 90% of owners (28/31) reported at least 1 change in their dog’s behavior including polyuria, polydipsia, polyphagia, and/or polypnea as the most common changes noted. Overall, 61% (19/31) of owners reported an increase in filling of the water bowl over baseline and one-third (11/31) of pet owners reported cleaning up urinary accidents for pets who had been continent prior to the start of the study. Pet owner steroid satisfaction remained high through day 14 at 4.5/5 (1= very unsatisfied, 5= very satisfied).
Conclusion: This study highlights the impact short term anti-inflammatory doses of prednisone or prednisolone have on dog behaviour and confirms the hypothesis that prednisone or prednisolone usage changes the behaviour of dogs included in this study as soon as day 5 post-treatment. By day 14, the majority of dogs experienced one or more behaviour changes, with polyuria and polydipsia most commonly reported. Adverse events were noted regardless of starting dosage or regimen. Although most pet owners expressed satisfaction with steroid treatment due to its high efficacy, 70% would select a more costly treatment if that treatment had fewer side effects.
The Effect of Empowerment Program to Reduce Sugar Consumption Based on the Multi-Theory Model on Body Mass Index and Abdominal Obesity in Iranian Women
Authors: Joveini Hamid , Sharifi Nader , Meymary Batool Kalate , Mehri Ali , Shahrabadi Reza , Rahmanian Vahid , Hashemian Masoumeh
Published: October 2022

Background Considering the prevalence of overweight and abdominal obesity in middle-aged women, this study was conducted to determine the effect of empowerment program to reduce sugar consumption based on the MTM on BMI and abdominal obesity in women aged 30-60 in Joven.
Methods This quasi-experimental study (include descriptive and interventional sections) was conducted on the Joven city in Khorasan Razavi province in northeastern Iran from October 2020 to August 2021. Sampling was performed as a multi-stage cluster. After a descriptive study, 128 women with the conditions to enter the intervention section of the study were selected. In the control group, 63 people and in the intervention group, 65 people were eligible to enter the study.
The educational intervention was performed in five 60-minute sessions for groups of 12 people. The instruments included the demographic questionnaire, sugar consumption checklist and researcher-made questionnaire based on MTM constructs. Before the intervention, one, three and six months after the intervention, the questionnaire was completed by both intervention and control groups also measurement of waist circumference and BMI were performed using standard instruments. The obtained data were analyzed by SPSS 17.
Results After the educational intervention, there was a significant difference between the intervention and control groups in all the MTM constructs. Also, six months after the educational intervention, BMI, waist circumference and amount of consumption of sugary substances decreased significantly in the intervention group (p<0.05).
Conclusion This study showed the effectiveness of designing the educational intervention based on the MTM in reducing the consumption of sugary substances in women aged 30-60.
Incidence and severity of immune-related hepatitis after dual checkpoint therapy is linked to younger age independent of herpes virus immunity
Authors: Zhang Zhen , Rafei-Shamsabadi David , Lehr Saskia , Buettner Nico , Diehl Rebecca , Huzly Daniela , Pinato David James , Thimme Robert , Meiss Frank , Bengsch Bertram
Published: October 2022

Background and Aims:
Dual immune checkpoint blockade (ICB) therapy can result in immune-related-adverse events (irAE) such as ICB-hepatitis. An expansion of effector-memory (TEM) CD4 T cells associated with antiviral immunity against herpesviridae was implicated in ICB-hepatitis. Notably, these memory subsets are frequently associated with age. Here, we sought to understand baseline patient, immune and viral biomarkers associated with the development of ICB-hepatitis to identify currently lacking baseline predictors and test if an expansion of TEM or positive serology against herpesviridae can predict ICB-hepatitis.
Methods
A discovery (n = 39) and validation cohort (n = 67) of patients with advanced melanoma undergoing anti-PD-1&anti-CTLA4 combination therapy (total n = 106) were analyzed for baseline clinical characteristics, occurrence of irAE and oncological outcomes alongside serological status for CMV, EBV and HSV. Immune populations were profiled by high-parametric flow cytometry (n = 29).
Results
ICB-hepatitis occurred in 59% of patients within 100days; 35.9% developed severe (CTCAE 3–4) hepatitis. Incidence of ICB-hepatitis was higher in the younger (< 55y: 85.7%) compared to older ( > = 55y: 27.8%) age group (p = 0.0003), occured earlier in younger patients (p < 0.0001). The association of younger age with ICB-Hepatitis was also observed in the validation cohort (p = 0.0486). Incidence of ICB-hepatitis was also associated with additional non-hepatic irAE (p = 0.018), but neither positive IgG serostatus for CMV, EBV or HSV nor TEM subsets despite an association of T cell subsets with age.
Conclusion
Younger age more accurately predicts ICB-hepatitis after anti-PD-1&anti-CTLA4 checkpoint therapy at baseline compared to herpes virus serology or TEM subsets. Younger patients should be carefully monitored for the development of ICB-hepatitis.
Transcriptome Analysis Reveals Auto-Inflammatory Genes of Chronic Nonbacterial Osteomyelitis (CNO) Based on The Machine Learning
Authors: Fu Zhuodong , Wang Xingkai , Zou Linxuan , Zhang Zhe , Lu Ming , Zong Junwei , Wang Shouyu
Published: October 2022

Objectives:
Chronic nonbacterial osteomyelitis (CNO) is an auto-inflammatory bone disorder. Since the origin and development of CNO involve many complex immune processes, resulting in delayed diagnosis and lack of effective treatment. Although bioinformatics analysis has been utilized to seek key genes and pathways of CNO, only a few bioinformatics studies that focus on CNO pathogenesis and mechanisms have been reported. This study aimed to identify key biomarkers that could serve as early diagnostic or therapeutic markers for CNO.
Methods:
Two RNA-seq datasets (GSE133378 and GSE187429) were obtained from the gene expression omnibus (GEO). Weighted gene co-expression network analysis (WGCNA) and differentially expressed genes (DEGs) analysis were conducted to identify the correlated genes associated with CNO. After that, the auto-inflammatory genes mostly associated with CNO were yielding based on the GeneCards database and the CNO prediction model, which was created by the LASSO machine learning algorithms. According to the receiver operating characteristic (ROC) curves, the accuracy of the model and auto-inflammatory genes was verified by utilizing external datasets (GSE7014). Eventually, we performed clustering analysis by ConsensusClusterPlus.
Results:
Totally, eighty CNO-related genes were identified, which were significantly enriched in the biological process of regulation of actin filament organization, cell-cell junction organization and gamma-catenin binding. The mainly enriched pathways were Adherens junction, Viral carcinogenesis and Systemic lupus erythematosus. Two auto-inflammatory genes with high expression in CNO samples were identified by combing an optimal machine learning algorithm (LASSO) with GeneCards database. The external validation dataset (GSE187429) was utilized for ROC analysis of prediction model and two genes, and the results have well validation efficiency. Then, we found that the expression of the two genes differed between clusters based on consensus clustering analysis. Finally, the ceRNA network of lncRNA and small molecule compounds of the two auto-inflammatory genes were predicted.
Conclusion:
Two auto-inflammatory genes, HCG18/has-mir-147a/UTS2/MPO axis and the signal pathways identified in this study can help us understand the molecular mechanism of CNO formation and provide candidate targets for the diagnosis and treatment of CNO.
Adherence to Baltic Sea diet and healthy Nordic dietary pattern and lower risk of age-related cataract: a case-control study from Iran
Authors: Rahmani Jamal , Nikkhah Mehrnaz , Clark Cain C. T. , Ghanavati Matin
Published: October 2022

Background
Age-related cataract (ARC) is a multifactorial and progressive disease that causes blindness globally. Dietary antioxidants like vitamins and carotenoids have been reported to attenuate oxidative stress the main cause of ARC. However, the association between adherence to dietary healthy dietary patterns and ARC has yet to be understood. Since, adherence to Nordic diet style measured by two indices including Healthy Nordic Food Index (HNFI) and Baltic Sea Dietary Score (BSDS) was associated to some chronic diseases, in this study we aimed to investigate the association between HNFI and BSDS and the odds of ARC in a case-control study, in Iran.
Methods
This hospital-based case-control study was conducted on 98 patients with cataract and 198 healthy controls selected based inclusion criteria. A valid 168-item food frequency questionnaire (FFQ) was used to assess dietary intake over last year. To investigate the association between HNFI and BSDS and risk of ARC, the logistic regression tests was performed.
Results
Individuals in case and control groups were significantly different in body mass index (BMI), history of diabetes, hypertension and family history of hypertension. The intake of vitamin C, vitamin A and vitamin D showed significant difference between two groups of study. The adherence to BSDS and HNFI were associated with lower risk of ARC in crude and adjusted models. After adjustment for potential cofounders, participants in the highest tertile of HNFI and BSDS (compared to the lowest tertile) had 99.1% and 74% lower ARC risk (OR = 0.09; 95% CI = 0.03–0.22 and OR = 0.26; 95% CI = 0.11–0.58, respectively).
Conclusion
Our results support previous findings on protective effects of dietary patterns rich in antioxidant on cataract. Adherence to Nordic eating style measured by BSDS and HNFI consist of fruits and root vegetables, whole grain, fish and health fats may reduce risk of ARC.
Treatment of pregnant and early postpartum women with severe and critical COVID-19: experience at a tertiary center
Authors: Barbosa Rodrigo Nacif , Braga Maria Aparecida , Costa Bárbara Braga , Peret Frederico Jose Amedee
Published: October 2022

Background:The management of acute respiratory failure during pregnancy is a poorly defined issue in the literature, especially regarding the use of the prone position and the appropriate time for delivery. This study describes our experience in treating pregnant and postpartum women with severe or critical coronavirus disease 2019 (COVID-19).Materials and Methods:This descriptive retrospective study included 25 pregnant and 4 postpartum women admitted to an ICU due to respiratory complications from COVID-19 from June 2020 to August 2021.Results:The mean maternal age was 33.6 years, and the median gestational age (GA) at admission was 33 weeks. Obesity was the most common comorbidity. The median time between symptom onset and ICU admission was 10 days, while the median length of ICU stay was 14 days. Invasive mechanical ventilation (IMV) was required in 16 (55.2%) patients for a median time of 16.5 days. Prone positioning (PP) was performed in 68.7% of the patients on IMV, and resulted in an expressive increase in arterial oxygen partial pressure to fractional inspired oxygen (PaO2/FiO2 ratio). Eleven (44%) pregnant women delivered during their ICU stay for obstetric or fetal reasons: of these, 2 (18%) developed postpartum hemorrhagic shock and 1 (9%) developed abdominal wall infection. None of the 25 pregnant women underwent delivery due to acute respiratory failure or in an attempt to avoid intubation. There were 2 fetal deaths, but no maternal or neonatal deaths.Conclusion:We observed favorable outcomes in pregnant and postpartum women with severe and critical COVID-19 admitted to our institution. This finding reinforces the effectiveness of PP in the treatment of hypoxemic respiratory failure secondary to COVID-19 in pregnant women undergoing IMV, and suggests that gestation should only be interrupted in cases of obstetric and fetal complications, provided the patient is stable, or when hypoxemia is refractory to PP.
The effects of P on wheat root-associated prokaryotic communities are dependent on soil P content, growth stage and variety
Authors: Feng Zhi han , Li Na , Li Xin , Xiao Min , Wang Yi qun , Li Lu , Jiang Yan qiong , Liu Chi , Yao Ming jie , Li Xiangzheng
Published: October 2022

Background
Wheat root-associated microbes play an indispensable role in helping plants obtain phosphorus (P) from soil. However, it is not clear how the effects of soil P on microorganisms vary with growth stage and variety. Therefore, revealing the interactive effects of soil P content, growth stage and variety on wheat root-associated microorganisms, which is important for further improving the P absorption strategy of plants.
Methods
Three wheat varieties were selected for pot experiment under three levels of soil P content, and sampling at seedling, flowering and mature stage, respectively. Using 16S rRNA gene profiling, we investigated the relative contribution of compartment niche, growth stage, variety and soil P content and their interaction effects on wheat root-associated prokaryotic communities.
Results
Wheat root-associated prokaryotic community assembly was mainly shaped by compartment niches. For each compartment niche, growth stage had higher contribution to the variations of prokaryotic communities than soil P content and wheat variety. The effects of P content on the prokaryotic communities varied by growth stage and variety. Meanwhile, the effects of wheat variety were also influenced by growth stage and soil P content. It is worth mentioning that low P content exacerbated the variation of prokaryotic communities among varieties.
Conclusions
This study revealed the interaction of host selection and soil P content on root-associated prokaryotic communities. The effects of soil P on wheat root-associated prokaryotic communities are dependent on P content, growth stage and variety. Our study provides a fundamental understanding on the relationship between soil P content and microorganisms.
Genome-wide analysis and Stress-Responsive Expression Profiling of the LEA (late embryogenesis abundant) Gene Family in Wild Peanut
Authors: Huanhuan Jiang , Tong Wang , Li Sainan
Published: October 2022

Late embryogenesis abundant (LEA) proteins generally accumulate in seeds during the later stages of maturation. However, LEA proteins also accumulate in response to abiotic stressors, particularly those involving cellular dehydration. LEA genes have been well-characterized in many plant species, but not in peanut. Here we studied the LEA genes in two wild peanut species (Arachis duranensis and Arachis ipaensis) in an effort to create a genetic resource for peanut crop improvement. Specifically, we examined AdLEA and AiLEA sequence characteristics, gene structure, evolutionary relationships, chromosomal localization, promoter cis-acting elements, and expression patterns in different tissues under low temperature, salinity, drought, and ABA stress. In all, we identified 65 AdLEA and 69 AiLEA genes representing all 8 LEA subfamilies, which were unevenly distributed across all 10 peanut chromosomes. The majority of LEA proteins were found to be highly hydrophilic. MEME analysis indicated that LEA gene motifs were conserved within groups, but not between groups. The LEA genes contained a diverse array of stress- and phytohormone-responsive cis-acting elements, with the AdLEA2-20 and AiLEA2-20 genes containing the greatest number of elements. Both AdLEA2-20 and AiLEA2-20 were upregulated in response to cold temperatures, drought, salinity, and ABA-exposure, although the dynamics were tissue-dependent. This study lays the foundation for future studies on the LEA gene family and abiotic stress in peanut, and our results will be invaluable for the genetic improvement of peanut by characterizing the genetic resources of wild peanut species.
Effect of chitosan coating enriched with denak essential oil on ‘Navel’ orange quality infected by Penicillium digitatum
Authors: Rafiee Somayeh , Ramezanian Asghar
Published: October 2022

Background
Green mold, Penicillium digitatum, reduces the postharvest quality of citrus fruit as one of the most economically important horticultural crops in the world. The effect of chitosan, denak essential oil and CH + DEO was investigated on the quality of ‘Navel’ orange fruit infected by P. digitatum.
Results
The results showed that all treatments except DEO, CH and CH + DEO were unusable after 14 days due to decay. Fruit treated with CH and CH + DEO showed the lowest respiration rate and weight loss during storage time. Also, treated fruit had the highest total soluble solids, titratable acidity, total phenol and ascorbic acid content, and total antioxidant activity during cold storage. There was no significant difference in appearance and quality between CH and CH + DEO at the end of the storage period.
Conclusions
These results suggested that the coating with CH and CH + DEO can be useful for controlling decay and maintaining the quality in ‘Navel’ orange during the postharvest period.
Chinese Shadow Puppeteering Virtualization Based on a Real-Time Finger Tracking System
Authors: Kong Anthony , Wang Xue
Published: October 2022

Purpose:It is not an alarmist statement that Chinese shadow puppeteering skill is on the verge of disappearing. The reasons for this situation are complex, such as the impact of diversified forms of entertainment, old-aged Chinese shadow puppeteers, lack of audiences and young inheritors…etc. In urge to safeguarding Chinese shadow puppeteering, this study addresses this pressing issue by using a real-time finger tracking system to control the Chinese shadow puppet's performance.Design/methodology/approach: Researchers conduct a contrast experiment in the study. Eight young adults join the workshop to manipulate real shadow puppets using joysticks first, then manipulate virtual shadow puppets by wearing wireless data tracking glove.Findings: The outcomes of this study suggest that controlling a shadow puppet using one hand is not an easy thing for young beginners. However, the real-time finger-tracking system could help lower the threshold for shadow puppeteering learning while bringing positive motivation for youngest to learn and engage with the virtual puppets. Due to innovative, interesting, and interactive technological elements that stimulate those youngest who grown up in digital era to enjoy and engage the virtual puppeteering.Originality/value: This paper contributes to design, technology, and Chinese Culture by offering the technical foundation for future study to apply shadow puppetry digital archives in various fields such as animation production and creation.