bluuebunny/crossref_metadata_2025 huggingface dataset:
Dach1 Extends Artery Networks and Protects Against Cardiac Injury
Authors: Raftrey Brian , Williams Ian M. , Rios Coronado Pamela E. , Fan Xiaochen , Chang Andrew H. , Zhao Mingming , Roth Robert , Trimm Emily , Racelis Raquel , D’Amato Gaetano , Phansalkar Ragini , Nguyen Alana , Chai Timothy , Gonzalez Karen M. , Zhang Yue , Ang Lay Teng , Loh Kyle M. , Bernstein Daniel , Red-Horse Kristy
Published: August 2021

Rationale:

Coronary artery disease is the leading cause of death worldwide, but there are currently no methods to stimulate artery growth or regeneration in diseased hearts. Studying how arteries are built during development could illuminate strategies for re-building these vessels during ischemic heart disease. We previously found that
Dach1
deletion in mouse embryos resulted in small coronary arteries. However, it was not known whether
Dach1
gain-of-function would be sufficient to increase arterial vessels and whether this could benefit injury responses.

Objective:

We investigated how Dach1 overexpression in endothelial cells affected transcription and artery differentiation, and how it influenced recovery from myocardial infarction.

Methods and Results:

Dach1 was genetically overexpressed in coronary endothelial cells in either developing or adult hearts using
ApjCreER.
This increased the length and number of arterial end branches expanded arteries during development, in both the heart and retina, by inducing capillary endothelial cells to differentiate and contribute to growing arteries. Single-cell RNA sequencing of endothelial cells undergoing
Dach1
-induced arterial specification indicated that it potentiated normal artery differentiation, rather than functioning as a master regulator of artery cell fate. Single-cell RNA sequencing also showed that normal arterial differentiation is accompanied by repression of lipid metabolism genes, which were also repressed by Dach1. In adults,
Dach1
overexpression did not cause a statistically significant change artery structure before injury, but increased the number of perfused arteries in the injury zone post-myocardial infarction.

Conclusions:

Our data demonstrate that increasing
Dach1
is a novel method for driving artery specification and extending arterial branches, which could be explored as a means of mitigating the effects of coronary artery disease.
Saving for Multiple Financial Needs: Evidence from Lockboxes and Mobile Money in Malawi
Authors: Aggarwal Shilpa , Brailovskaya Valentina , Robinson Jonathan
Published: August 2021

We test whether the provision of multiple labeled savings accounts affects savings and downstream outcomes in an experiment with 761 microentrepreneurs in urban Malawi. Treatment respondents received one or multiple savings accounts, in the form of lockboxes or mobile money. We find that while providing additional boxes increased savings by 40%, technical issues marred the efficacy of a second mobile money account. Data from novel high-frequency surveys suggest that both types of accounts had impacts on downstream outcomes, including farming decisions and credit extended to customers. We do not detect differential downstream effects by the number or modality of accounts.
The Cost of Privacy: Welfare Effects of the Disclosure of COVID-19 Cases
Authors: Argente David , Hsieh Chang-Tai , Lee Munseob
Published: August 2021

South Korea publicly disclosed detailed location information of individuals who tested positive for COVID-19. We quantify the effect of public disclosure on the transmission of the virus and economic losses in Seoul. The change in commuting patterns due to public disclosure lowers the number of cases by 60,000 and the number of deaths by 2,000 in Seoul over two years. Compared to a city-wide lockdown that results in the same number of cases over two years as the disclosure scenario, the economic cost of such a lockdown is almost four times higher.
Exhibiting Faith against an Imperial Background: Angola and the Spiritans at the Vatican Missionary Exhibition (1925)
Authors: Amaral Ana Rita
Published: August 2021

In 1925 the Vatican Missionary Exhibition took place, presenting thousands of objects sent by Catholic missions around the world. Resulting from substantial efforts by the Church, the exhibition had a significant public impact, with an estimated one million visitors. It marked a critical moment in the international affirmation of the Church, as well as the reformulation and expansion of its missionary policy in the aftermath of the Great War.

Catholic missions and congregations in the Portuguese colonial empire participated in the exhibition. This article focuses on the Angolan case, where the Congregation of the Holy Spirit was the main protagonist of Catholic missionisation. I examine the organisation process, the circulation of norms and objects across imperial borders, and their exhibition at the Vatican. I discuss the tensions between the pontifical message and Portuguese missionary politics, as well as the intermediary position that the Spiritans occupied.
What Is Religion in Africa? Relational Dynamics in an Entangled World
Authors: Meyer Birgit
Published: August 2021

Addressing the implications of the introduction of the concept of religion to Africa in the colonial era, this essay approaches religion from a relational angle that takes into account the connections between Africa and Europe. Much can be learned about the complexity and power dynamics of these connections by studying religion not simply in but also from Africa. Referring to historical and current materials from my research in Ghana by way of example, my concern is to show how a focus on religion can serve as a productive entry point into the longstanding relational dynamics through which Africa and Europe are entangled. This is a necessary step in decolonizing scholarly knowledge production about religion in Africa, and in religious studies at large.
An Econometric Analysis of Maize Farmer’s Choice of Land Ownership Systems: Evidence Using Panel Data from Tanzania
Authors: Lasway Jovin A. , Selejio Onesmo
Published: August 2021

This paper determines the socioeconomic and physical characteristics that influence maize farmer’s choice of land ownership systems in Tanzania, i.e., owned, sharecropped, and rented title land. The paper uses the Tanzania National Panel Survey (TZNPS) data basing on 2,073 observations comprising of a sample size of 691 households in three consecutive waves 2008/2009, 2010/2011, and 2012/2013. Using a t-test for mean comparison, the paper found that there is a significant difference between male and female-headed households across socioeconomic and physical factors determining the choice of land ownership systems among maize smallholder farmers. Moreover, the paper findings revealed that 90 percent, 7 percent, and 3 percent of the sampled maize smallholder farmers chose to farm under-owned, shared, and rented title land system respectively. Furthermore, the econometric analysis which is grounded from the multinomial logit regression model showed that accessibility, farm size, quantity harvested, accessibility of extension services, gender, household size, and age are all pertinent in influencing the smallholder maize farmer’s choice of land ownership. The paper recommends that these characteristics of the smallholder maize producers should be critically considered when formulating any land and agricultural policies to enhance proper decision making by smallholder farmers on the choice of land ownership system that may led to sustainable agricultural production, productivity, and food security in developing countries particularly in Tanzania.
Multisensory Effects on Illusory Self-Motion (Vection): the Role of Visual, Auditory, and Tactile Cues
Authors: Murovec Brandy , Spaniol Julia , Campos Jennifer L. , Keshavarz Behrang
Published: August 2021

A critical component to many immersive experiences in virtual reality (VR) is vection, defined as the illusion of self-motion. Traditionally, vection has been described as a visual phenomenon, but more recent research suggests that vection can be influenced by a variety of senses. The goal of the present study was to investigate the role of multisensory cues on vection by manipulating the availability of visual, auditory, and tactile stimuli in a VR setting. To achieve this, 24 adults (Mage = 25.04) were presented with a rotating stimulus aimed to induce circular vection. All participants completed trials that included a single sensory cue, a combination of two cues, or all three cues presented together. The size of the field of view (FOV) was manipulated across four levels (no-visuals, small, medium, full). Participants rated vection intensity and duration verbally after each trial. Results showed that all three sensory cues induced vection when presented in isolation, with visual cues eliciting the highest intensity and longest duration. The presence of auditory and tactile cues further increased vection intensity and duration compared to conditions where these cues were not presented. These findings support the idea that vection can be induced via multiple types of sensory inputs and can be intensified when multiple sensory inputs are combined.
Orienting Auditory Attention through Vision: the Impact of Monaural Listening
Authors: Turri Silvia , Rizvi Mehdi , Rabini Giuseppe , Melonio Alessandra , Gennari Rosella , Pavani Francesco
Published: August 2021

The understanding of linguistic messages can be made extremely complex by the simultaneous presence of interfering sounds, especially when they are also linguistic in nature. In two experiments, we tested if visual cues directing attention to spatial or temporal components of speech in noise can improve its identification. The hearing-in-noise task required identification of a five-digit sequence (target) embedded in a stream of time-reversed speech. Using a custom-built device located in front of the participant, we delivered visual cues to orient attention to the location of target sounds and/or their temporal window. In Exp. 1 (), we validated this visual-to-auditory cueing method in normal-hearing listeners, tested under typical binaural listening conditions. In Exp. 2 (), we assessed the efficacy of the same visual cues in normal-hearing listeners wearing a monaural ear plug, to study the effects of simulated monaural and conductive hearing loss on visual-to-auditory attention orienting. While Exp. 1 revealed a benefit of both spatial and temporal visual cues for hearing in noise, Exp. 2 showed that only the temporal visual cues remained effective during monaural listening. These findings indicate that when the acoustic experience is altered, visual-to-auditory attention orienting is more robust for temporal compared to spatial attributes of the auditory stimuli. These findings have implications for the relation between spatial and temporal attributes of sound objects, and when planning devices to orient audiovisual attention for subjects suffering from hearing loss.
Megarthrus of China. Part 5. The M. calcaratus complex, with description of three new species (Coleoptera: Staphylinidae: Proteininae)
Authors: LIU ZHIPING , CUCCODORO GIULIO
Published: August 2021

Three new species of Megarthrus Curtis from mainland China belonging to the M. calcaratus species complex are described: M. bajie Liu & Cuccodoro sp. nov., M. wujing Liu & Cuccodoro sp. nov., and M. wukong Liu & Cuccodoro sp. nov.. Also pertaining to this lineage of Megarthus, the East Palaearctic M. zerchei Cuccodoro & Löbl is reported here to occur in China (new country record). These four species are diagnosed, illustrated, and keyed. Their distribution in mainland China is mapped, and their affinities are briefly discussed.
Review of the genus Aschistophleps Hampson, 1893 (Lepidoptera, Sesiidae) of Laos
Authors: GORBUNOV OLEG G.
Published: August 2021

The genus Aschistophleps Hampson, 1893 is reviewed for Laos. A. longipoda Arita & O. Gorbunov, 2000 is reported for the country for the first time. Two new species A. zamesovi sp. nov. and A. nahinensis sp. nov. are described and figured. The mud-puddling behaviour of males of these species are discussed. All material, including the types of new described species are deposited in the collection of the A.N. Severtsov Institute for Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia (COGM).
Hidden in the plain sight: a new species of Rhabdophis (Serpentes: Natricinae) from the Rhabdophis himalayanus complex
Authors: DAS ABHIJIT , SMITH ERIC N. , SIDIK IRVAN , SARKER GOUTAM C. , BORUAH BITUPAN , PATEL NAITIK G. , MURTHY B.H.C.K , DEEPAK V.
Published: August 2021

A new species of natricine snake of the genus Rhabdophis is described from Northeast India. Morphologically the new species is similar to Rhabdophis himalayanus but it can be distinguished from it by lacking a nuchal groove and nuchal glands, being relatively smaller, and having a distinct neck, ventral, and subcaudal coloration. In overall coloration it resembles R. chrysargos, from which it is distinguished by several aspects of its morphology and genetic differences. The discovery of Rhabdophis bindi sp. nov. reveals that forested regions in the northeastern part of India needs further biological exploration.
Color Stability and Translucency of Polymer-Infiltrated Ceramic-Network Materials—A Systematic Review of the Literature
Authors: Labban Nawaf
Published: August 2021

This systematic review aimed to compile information and report the color stability and translucency of polymer-infiltrated ceramic-network (PICN) materials for suitable clinical applications in dentistry. Original peer-reviewed articles in electronic databases including PubMed, MEDLINE,
Scopus, and Web of Science were searched to identify relevant papers published between January 2013 and August 2020. The published papers were only in the English language and limited to in vitro studies, and experimental reports were considered. The data from nine studies were considered
when reviewing the color stability and translucency of PICN in comparison to other commonly used restorative materials. Among the included studies, color stability assessment was performed in five studies, and the other four studies were involved in the combined assessment of both color stability
and translucency. PICN materials are a comparatively newer class of restorative materials and are recommended to overcome the drawbacks of ceramics and resin composites. The outcomes of previous laboratory studies still do not provide convincing results regarding the optical properties of
PICN materials. However, with existing sources and evidence, PICN materials exhibit less color stability than ceramics do but better color stability compared to that of resin nanoceramics (RNC) and resin composites. The translucency of PICN materials is low compared to that of ceramics, nanoceramics,
and resin composites.
Effect of Sirtuin1 (Sirt1) on Bone Marrow Mesenchymal Stem Cells (BMSCs) Osteogenesis/Adipogenesis via β-Catenin/The Transcription Factor T Cell Factor 1 (TCF1)/Runt-Related Transcription Factor 2 (RUNX2)
Authors: Shi Wenji , Zhao Mingxing , Shi Guangxia
Published: August 2021

Bone marrow mesenchymal stem cells (BMSCs) have self-renewal potential. Sirt1 regulates cell differentiation and apoptosis. However, Sirt1’s effect on BMSCs osteogenic/adipogenic differentiation has not been fully elucidated. SD rats were randomly divided into Osteoporosis (OP)
group and sham operation group. OP rat BMSCs were isolated and assigned into control group, NC group and Sirt1 siRNA group followed by analysis of Sirt1 level by Real-time PCR, cell proliferation by MTT assay, expression of OC, OPN and FABP4 level by real time PCR, and β-Catenin/TCF1/Runx2
protein expression by Western blot. In OP group, Sirt1 expression was significantly increased and BMSCs proliferation was decreased along with reduced OC and OPN mRNA expression, increased FABP4 expression and reduced β-Catenin/TCF1/Runx2 expression compared with sham operation
group (P < 0.05). In Sirt1 siRNA group, Sirt1 expression was significantly reduced, BMSCs proliferation was increased, OC and OPN mRNA expression was increased, FABP4 expression was decreased, and β-Catenin/TCF1/Runx2 expression was increased compared to OP group (P
< 0.05). Sirt1 is increased in osteoporosis. Down-regulating Sirt1 in osteoporotic BMSCs can regulate β-Catenin/TCF1/Runx2 signaling and promote BMSCs osteogenic differentiation and inhibit adipogenic differentiation.
Anti-Osteoporosis Effect of Heme on Osteoblast Pre-Cells and Rat Model
Authors: Jiang Hong-Qiang , Deng Yao , Han Chao , Ma Xin-Long
Published: August 2021

Objectives: Osteoporosis is a metabolic bone disease caused by various factors. As a prosthetic group of haemoglobin, heme can promote the formation of blood vessels and the regeneration of related cells by increasing the proliferation level of endothelial cells and reticulocytes.
This study observed the anti-osteoporosis effect of heme on preosteoblast (POB) cells and rat models. Methods: Heme mimics were transfected into POB cells of patients in stable culture, and the effect of heme transfection on the activity of POB cells was detected via the cell scratch
test and cell migration assay. 45 SD female rats were randomly divided into group A, the rats were only treated with surgery (n = 15); group B, the rats were given 30 mg/kg of heme chloride by tail vein injection on the 1st, 7th, and 14th days after surgery (n = 15); group C,
rats were injected with the same amount of saline in the tail vein (n = 15). The mRNA and protein expressions of alkaline phosphatase (ALP), bone-specific alkaline phosphatase (B-ALP), and type I collagen C-terminal peptide (CTX) in rats serum were detected via western blot and real-time
PCR (real-time polymerase chain reaction). The expressions of IL-6 and TNF-α in rats serum were detected via Elisa. The pathological changes in the morphology of distal femur were observed by Hematoxylin-eosin staining. Results: After transfected with heme mimics, the proliferation,
migration and invasion ability of the patient’s cells increased significantly, and the apoptosis rate of the cells decreased significantly. The relative expression levels of ALP and CTX mRNA and protein in serum of osteoporosis patients and rat significantly decreased 24 hours after
transfection of heme mimics (p < 0.05), while B-ALP significantly increased. The expression of IL-6 and TNF-α in the rat model of osteoporosis group significantly increased (p < 0.05), but after transfection with heme mimic, the expression of IL-6 and TNF-α
in the rat reduced significantly. HE showed that, after transfection, the femoral trabeculae were substantially broader and thicker, and the number also increased significantly. The number of trabecular fractures and fat cells was decreased, the trabecular bones were smoother and close to
the control group. Conclusion: Heme can significantly promote the proliferation, migration and invasion of POB cells in the body, and also can achieve the anti-osteoporosis effect by adjusting the expression of ALP, B-ALP, and CTX, IL-6 and TNF-α in osteoporosis model of rat.
Protective Effect of Butylphthalide on Neuronal Apoptosis in Parkinson Rats and Its Effect on miR-146a-5p Expression and Phosphatidylinositide 3-Kinases/Protein Kinase B Pathway
Authors: Mai Rongkang , Cao Yiyao , Yu Huitian , Zheng Yong , Huang Juke
Published: August 2021

80 male Wistar rats were stochastically assigned to Sham + Vehicle group, Sham + BUT group, PD + Vehicle group and PD + BUT group. Rotenone PD model rats were prepared by subcutaneous injection of rotenone sunflower oil emulsion 2 mg/(kg · d) for 5 consecutive weeks. Butylphthalide
80 mg/(kg · d) were given to the rats in Sham + BUT group and PD + BUT group by gavage from the first day of rotenone injection for 5 weeks. Subsequently, the motor retardation ability and the morphological changes of the substantia nigra (SN) of each group were evaluated. Meanwhile,
the levels of neuronal injury, apoptosis, inflammation and oxidative stress in each group of rats were assayed. The impact of BUT treatment on miR-146a-5p expression and PI3K/AKT signal pathway in rat brain tissue was assayed. Finally, by constructing a PD cell model of the neurotoxin 6-hydroxydopamine
(6-OHDA)-treated human neuroblastoma cell line SH-SY5Y, the in vitro anti-PD pharmacological effect of BUT was further verified.
MiR-153 Enriched in Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Promotes Chemotherapy Sensitivity of Papillary Thyroid Carcinoma
Authors: Wang Xiaoxin , Zheng Jia , Wen Donghu , Li Chunxiang , Li Xingjiang
Published: August 2021

Our study assesses the effect of bone marrow mesenchymal stem cells (BMSCs) exosomes miR-153 on papillary thyroid carcinoma (PTC). Adipogenesis and osteogenic induction of MSCs was performed and labeled with Cy5 labeled miR inhibitor. Cells were transfected followed by analysis of miR-153
level by real-time PCR, P-gp level by immunoblotting, and cell viability. MSCs are non-hematopoietic bone marrow-derived cells and symmetrical fibroblasts have the same characteristics as MSCs. MSCs have the potential for adipogenesis and osteogenic differentiation; miR-Cy5 can only enter
PTC cells through vesicle transfer. TMZ treatment upregulated miR-153 in exosomes; MSC-derived exosomes can be directly transferred to PTC cells. miR-153-inhibitor-Cy5 can effectively inhibit miR-153 transcription and expression of resistance-related proteins. miR-153-inhibitor can promote
TMZ’s effect and lead to cell death as demonstrated by increased level of active caspase-3. Inhibiting the endogenous transcription of miR-153 by miR-153 inhibitor can significantly down-regulate cell resistance protein, thereby promoting cell apoptosis under the action of TMZ.
Bone Marrow Mesenchymal Stem Cell (BMSC)-Derived Exosomes Increase Colon Cancer Cell Apoptosis and Inhibit Proliferation and Migration
Authors: Zhang Xinfa , Han Cheng
Published: August 2021

This study aims to investigate whether bone marrow mesenchymal stem cell (BMSC) exosomes (BMSC-exos) affects the progression of colon cancer. Ultracentrifugation was used to extract and collect BMSC-exos which were assessed under electron microscope and by flow cytometry. The BMSCs
were divided into two groups: control group treated with α-MEM basal medium and experimental group with exosomes (10 μg/ml). Exos were extracted from BMSCs and co-cultured with colon cancer cells, followed by analysis of cell viability by CCK-8 assay and GLUT3 mRNA and
protein expression by RT-qPCR and Western blot. The electron microscope analysis indicated that the primary BMSCs showed a long spindle shape with a negative expression of antigen CD34 and positive antigen CD90. Importantly, exos inhibited the viability of colon cancer cells HCT116 and decreased
the expression of GLUT3, suggesting that exos might increase the colon cancer cell apoptosis. In conclusion, BMSC-exos inhibit cell progression in colon cancer and might be served as a promising biomarker.
Arthroscopic Two-Point Suture Bridge Fixation Technique for the Treatment of Anterior Cruciate Ligament Tibial Avulsion Fractures
Authors: Zheng Dong , Yin Jianjian , Han Long , Gui Jianchao
Published: August 2021

This study aimed to present and evaluate a new arthroscopic technique that uses two-point suture fixation for anterior cruciate ligament (ACL) tibial avulsion fractures. A total of 15 patients diagnosed with ACL tibial avulsion fracture underwent arthroscopic suture fixation from November
2018 to October 2019 and were treated using two-point suture fixation. The patients were followed up and evaluated according to Lysholm scores, International Knee Documentation Committee (IKDC) subjective scores, Tegner activity level scales, anterior drawer testing, and KT-1000 arthrometer
testing. The mean follow-up period was 18 months (12 to 24). All patients had a negative Lachman test and anterior drawer test at final follow-up and showed the radiological union of avulsion fracture at 12-week postoperative radiograph. The Lysholm score improved significantly postoperatively
with a mean score of 94.26±3.63 (87 to 98; p < 0.001). The Tegner score improved significantly postoperatively from 3.61 ±1.37 to 7.14±1.51 (P < 0.001). The KT-1000 measured value decreased significantly postoperatively from 7.3±1.5 to 1.4 ±1.2
(P < 0.001). The IKDC category was abnormal or severely abnormal preoperatively, and all patients improved to normal or nearly normal at final follow-up. Arthroscopic treatment using the two-point suture fixation technique is effective for ACL avulsion fracture and can restore the
function and stability of the knee joint.
Metformin Weakens the Angiogenic Potential of Human Bone Marrow Mesenchymal Stem Cells (hBMSCs) by Activating Mammalian Target of Rapamycin (mTOR) Signaling
Authors: Chen Lihua , Shi Ji’e , Qu Xiu
Published: August 2021

To study metformin’s effect on the angiogenesis of human bone marrow mesenchymal stem cells (hBMSCs). Cells were treated with metformin (0.5, 1, 10, 50, 100, 200 and 500 βM) for 14 days, followed by analysis of cell viability and total fatty acid profile, level of
VEGFR-2, Tie-2, VE-Cadherin and mTOR signaling protein, cell differentiation by microtubule generation and cell migration by transwell assay. Metformin dose dependently decreased cell survival and reduced palmitate, oleate, stearate and linoleate content. In addition, it downregulated VEGFR-2
and Tie-2 and decreased the angiogenic potential of BMSCs and down-regulated VE-Cadherin. Western blot and PCR analysis showed that metformin activated mTOR signaling and up-regulated the transcription of autophagyrelated genes. Metformin can reduce BMSCs angiogenic potential by regulating
mTOR signal pathway.
Preparation and Evaluation of the Curative Effect of Blue Shark (Prionace glauca) Skin Collagen Composite Gel in a Rat Oral Ulcers Model
Authors: Huang Meineng , Jiang Sheng , Chen Tong , Han Xu , Yang Xinyu , Quan Zhizen , Yuan Yang , Li Jing , Deng Kehan , Li Tiejun , Cao Zhizhong , Wei Yibo
Published: August 2021

Objective: To evaluate the curative effect of blue shark skin collagen composite gel on oral mucosal ulcer using the rat oral ulcers model stimulated by glacial acetic acid. Methods: Collagen from blue shark skin was isolated and physiochemically characterized by FTIR,
SDS-PAGE and scanning electron microscopy (SEM). Seventy standard male rats were divided into seven groups. The surface and the area of the ulcer were observed and calculated daily. After 12 days of administration, rats in the model group and the control group were killed and the ulcer and
surrounding tissues were cut to pieces about one mm3 size. The specimens were stained with 10% formalin solution, paraffinembedded sections, HE staining and light microscope were used to observe the histopathological changes in ulcer tissues. Results: The high-dose group
had the fastest ulcer healing effects after 12 days of treatment with blue shark skin collagen composite gel. The composite gel was found to significantly accelerate the healing of oral ulcers in a dose-dependent manner. Conclusion: The blue shark skin collagen composite gel in this
study may be a good biomedical material candidate for the treatment of oral ulcers in the near future. Potential of other marine fish skin collagen comples on healing oral ulcers should be also considered.
Role of MicroRNA-101 on Proliferation and Migration of Prostate Cancer Cells
Authors: Weng Yiming , Xiang Jun , Le Wei , Mao Yuanshen
Published: August 2021

Background: MicroRNA-101 is a tumor inhibitor that stimulates tumor progression by reducing or inhibiting the expression of certain oncogenes. Some studies presented that cox-2 is target of MicroRNA 101 in prostate cancer process. Methods: MicroRNA-101 expression was detected
by RT-PCR in PC3 cell lines. A and to determine cell proliferation we used MTT assays. Cell would heal and Flow cytometry assays were also used to detect cellular migratory ability and apoptosis, respectively. To assess cox-2 protein expression, Immunohistochemistry was used and data analyzed
by data analysis by SPSS 20. Results: PC3 cells treated by MicroRNA-101 mimics displayed a 24% elevation in growth rate compared with blank (P < 0.01) at 48 h, and a 12% increase (P < 0.01) at 72 h. On the other hand, at 48 and 72 h after the MicroRNA-101 inhibitor
transfection, proliferation of PC3 cell was decreased significantly. The early apoptosis rate in transfected PC3 cells with MicroRNA-101 mimic (74.4%) and inhibitor (22.8%) were significantly different at 72 h after transfection (P < 0.05), MicroRNA-101 mimics inhibited cell migration,
adhesion, and spread was wider relative to the group of control and inhibitor for the PC3 cells. Expression of Cox-2 in transfected PC3 with the MicroRNA-101 inhibitor was higher than the mimic and control groups significantly (P < 0.01). Conclusion: MicroRNA-101 by Cox-2
can play key roles in the prostate cancer pathogenesis.
The Maximum Likelihood Ensemble Filter with State Space Localization
Authors: Zupanski Milija
Published: August 2021

A new method for ensemble data assimilation that incorporates state space covariance localization, global numerical optimization, and implied Bayesian inference is presented. The method is referred to as the MLEF with state space localization (MLEF-SSL) due to its similarity with the maximum likelihood ensemble filter (MLEF). One of the novelties introduced in MLEF-SSL is the calculation of a reduced-rank localized forecast error covariance using random projection. The Hessian preconditioning is accomplished via Cholesky decomposition of the Hessian matrix, accompanied with solving triangular system of equations instead of directly inverting matrices. For ensemble update, the MLEF-SSL system employs resampling of posterior perturbations. The MLEF-SSL was applied to Lorenz model II and compared to ensemble Kalman filter with state space localization and to MLEF with observation space localization. The observations include linear and nonlinear observation operators, each applied to integrated and point observations. Results indicate improved performance of MLEF-SSL, particularly in assimilation of integrated nonlinear observations. Resampling of posterior perturbations for an ensemble update also indicates a satisfactory performance. Additional experiments were conducted to examine the sensitivity of the method to the rank of random matrix and to compare it to truncated eigenvectors of the localization matrix. The two methods are comparable in application to low-dimensional Lorenz model, except that the new method outperforms the truncated eigenvector method in case of severe rank reduction. The random basis method is simple to implement and may be more promising for realistic high-dimensional applications.
Usefulness of Diagnostic Staging Laparoscopy for Advanced Gastric Cancer
Authors: Yoshikawa Kozo , Shimada Mitsuo , Higashijima Jun , Tokunaga Takuya , Nishi Masaaki , Takasu Chie , Kashihara Hideya , Eto Shohei , Yoshimoto Toshiaki
Published: August 2021

Background

For advanced gastric cancer (AGC), peritoneal metastasis is the most common determinant of unresectability, but accurate preoperative diagnosis for peritoneal metastasis is challenging. Staging laparoscopy (SL) can detect unsuspected peritoneal metastasis. This study retrospectively evaluated the utility of SL and its indication in patients with AGC.

Methods

In this study, we enrolled 114 patients with pathologically diagnosed gastric adenocarcinoma who underwent SL.

Results

Of the 114 patients, 43 (37.7%) had peritoneal metastasis (P1 or CY1). Higher age, larger tumor size, type 4 GC, deeper tumor depth, elevated CA125, and ascites findings in preoperative CT were found to be significant predictors of peritoneal metastasis. In multivariate analysis, peritoneal metastasis was associated with type 4 GC (odds ratio [OR]: 6.11; 95% confidence interval [CI]: 1.87-19.8; P < .01) and ascites in CT (OR: 4.25; 95% CI: 1.48-12.1; P < .01).

Conclusions

Staging laparoscopy is an effective tool to detect peritoneal metastasis from AGC. It can increase the curative resection rate and decrease unnecessary laparotomies.
Multi-System Inflammatory Syndrome Presenting as Non-Specific Colitis: A Medical Diagnosis with a Surgical Presentation
Authors: Bingmer Katherine E. , Ebertz David P. , Violette Aisha K. , Radow Brandon S. , Rushing Amy P. , Loudon Andrew M. , Moorman Matthew L.
Published: August 2021

A 20-year-old woman with previous COVID-19 diagnosis presented with abdominal pain and colitis on CT scan. She was admitted in septic shock, with etiology of colitis unclear. After resuscitation, antibiotics, and steroids, she clinically deteriorated. Worsening Clostridioides difficile infection was most likely and she was taken to the operating room. Intraoperatively, only a segment of transverse colon appeared abnormal on gross and endoscopic evaluation. Total colectomy was deferred in favor of segmental resection. Given her unusual disease pattern and recent COVID-19 infection, diagnosis of MIS-C was considered. Steroids were continued and treatment broadened to include heparin and IVIG. The patient returned to the operating room for planned reexploration, endoscopy, and end colostomy. On hospital day three, the patient had an acute mental status change. Computed tomography demonstrated acute cerebral edema with brainstem herniation. The family chose comfort-care measures. Final pathology from the transverse colon demonstrated COVID-19-associated vasculitis
Analysis of Psychological Assessments Affecting Patients Undergoing Treatment for Chest Wall Deformities
Authors: Alqadi Gratiana O. , Saxena Amulya K.
Published: August 2021

Aim

This study aimed to review the literature with regard to the psychological aspects in patients with pectus excavatum (PE) and pectus carinatum (PC).

Methods

The literature was reviewed by analyzing articles on PubMed using the search terms “psychology” and “pectus.”

Results

The literature search revealed 22 articles that offered a total of 2214 patients for analysis. Regarding chest wall deformities (CWD), there were 15 articles referring to PE, 4 articles on PC, and 3 pertaining to both PE and PC. Authors used various types of questionnaires and 14 studies which also included a parent questionnaire. There are 14 reports which analyzed both the preoperative and postoperative psychological status and 4 reports in which a control group was used. Treatment of CWD was shown to have consistently positive results. There is no consensus regarding the correlation between severity of CWD and psychological issues. Only 2 studies found no statistically significant results after treatment.

Conclusions

The literature reveals a great concern for psychosocial issues in CWD patients. Most articles describe improvement in overall quality of life (QOL) after surgery. An increase in social and physical function, body image, and self-esteem was observed in CWD patients, which is supported by parent responses.
Designing a developmental progression to assess students’ conceptual understandings by focusing on the language demands in Science
Authors: Panizzon Debra , Pegg John , Arthur Dagmar , McCloughan Gerry
Published: August 2021

Few would argue the value of learning progressions in providing useful structures for selecting and sequencing in a developmental manner the key components of an ‘intended curriculum’. Yet, there are pervading issues around what is meant by a developmental sequence, along with how they are used to assess what learners know, understand and can do. One key oversight in Science is recognising the role of technical and non-technical language in student conceptual development. This article reports on the construction of a hypothesised learning progression that identifies students’ progress in understanding essential concepts in the Chemical Sciences from Foundation to Year 6. It is based upon an extensive analysis of the technical and non-technical language of the Australian Curriculum: Science. The progression was constructed by focusing upon learner-appropriate language and scientific understanding with the Structure of the Observed Learning Outcome model ( Pegg, 2018 ) providing the theoretical basis for ensuring systematic and objective rigour in the resultant developmental progression.
Adaptation and Validation of a Portuguese Version of the Sports Motivation Scale-II (SMS-II-P) Showing Invariance for Gender and Sport Type
Authors: Rodrigues F. , Pelletier L. , Rocchi M. , Cid L. , Teixeira D. , Monteiro D.
Published: August 2021

In the present cross-sectional study, we adapted and examined the validity of a Portuguese version of the Sport Motivation Scale II (SMS-II-P) within a sample of 1148 Portuguese athletes (women = 546, men = 602) with a mean age of 18.45 years ( SD = 5.36), participating in a variety of sports (i.e., football, basketball, swimming, and athletics). We conducted confirmatory factor analysis, convergent and discriminant validity analysis, and multigroup analysis across participants’ sport type (team and individual) and gender. We also examined the correlations between the SMS-II-P behavioral regulations and basic psychological needs satisfaction. The results supported that the SMS-II-P had good psychometric properties and was invariant across gender and sport type. The scale demonstrated good convergent and discriminant validity, and the subscales achieved adequate internal consistency. Correlations between the six types of regulation measured in the SMS-II supported the distinction between autonomous and controlled behavioral regulations, and the correlations between these subscales and other measures of autonomy, competence, and relatedness satisfaction provided evidence of the self-determination continuum. Implications of this research for assessing Portuguese athletes and conducting future research are discussed.
Authoritarianism and Immigration Attitudes in the UK
Authors: Peresman Adam , Carroll Royce , Bäck Hanna
Published: August 2021

Opposition to immigration has featured prominently in the “cultural backlash” to globalization in Western nations and was a key determinant of Britain’s Brexit referendum. In this article, we draw on theories of intergroup threat to examine the effect of “right-wing authoritarianism” on immigration attitudes in the UK. Previous research suggests that cultural aspects of immigration are especially important in shaping anti-immigrant attitudes. We use an original survey measuring attitudes toward immigration from differing skill levels and national origins. We find that right-wing authoritarianism is a much stronger predictor of immigration attitudes than other attributes. In addition, the effect of right-wing authoritarianism varies by immigrant origin, most strongly predicting opposition toward immigrant groups that may be perceived as culturally distant. We also find evidence that these effects are driven by the “aggression” component of right-wing authoritarianism, a facet of authoritarianism that captures a predisposition toward the enforcement of group norms.
How Does Organizational Compassion Motivate Employee Innovative Behavior: A Cross-Level Mediation Model
Authors: Guo Yungui , Zhu Yanting
Published: August 2021

This study aims to explore the mediating mechanisms between organizational compassion and employee innovative behavior by introducing perceived insider status and felt responsibility for constructive change (FRCC) as mediating variables. Drawing on cognitive-affective personality system theory, a cross-level mediation model was established. Data were collected via the online-based questionnaire from 420 employees of 12 enterprises in China. Multilevel path modeling was leveraged to examine hypotheses proposed in this study. The results indicate that FRCC mediates the relationship between organizational compassion and employee innovative behavior. Moreover, perceived insider status and FRCC serially mediate the relationship between organizational compassion and employee innovative behavior. This study has significant theoretical and practical implications as it is the first study to investigate the value of organizational compassion to employee innovative behavior at the workplace.
Seismic response of CRTS II ballastless track-bridge system considering the damage of track structure
Authors: Yan Bin , Li Zhe , Liu Shi , Xie Hao-Ran
Published: August 2021

Based on the 16–32 m simply-supported beam bridge on Shanghai-Kunming high-speed railway, a collision model of CRTS II track system was established. The model considered the longitudinal, horizontal as well as vertical nonlinear constraints among structural layers, and the Kelvin element was used to simulate the pounding effect of the gaps. The seismic response of the CRTS II track system was firstly analyzed and then the influences of different cases of damaged track plate, base plate, and mortar layer were discussed. The study revealed that: (1) The rail, track plate, and base plate all bear large longitudinal force and their stress envelope curves are all anti-symmetric. (2) The broken track plates result in a sharp decrease in track plate stress and an extreme increase in rail stress and base plate stress near the gap. The broken base plates result in a sharp decrease in base plate stress and an extreme increase in rail stress and track plate stress near the gap. (3) Both the pounding frequency and pounding force between broken slabs are relatively large and will decrease after some time. (4) The broken slabs near ends of the first bridge span greatly increase the pounding force of stoppers close to the abutment. (5) The gap width has a huge influence on the pounding force and times of stoppers and gaps. (6) The debonding of mortar layer has a great influence on the vertical displacement of rail, track plate, and the base plate.
Subverting the Electronic Language Textbook to Make it Relevant
Authors: Bloom Sam W.
Published: August 2021

The current article was inspired by two incongruous phenomena: the perception of the lack of gender sensitivity and inclusiveness in a particular language textbook and the coronavirus disease pandemic. As we went completely remote over the past 2.5 semesters, students were encouraged to address those issues via chat and voice modalities. If there is a silver lining to the tragic events of the past 15 months, it was that remote, synchronous discussion was vastly more participatory than in-class discussion with regard to those and other issues. I argue that, in designing our textbook-dependent courses, we can use such deficiencies to our advantage through some of the tools that many of us were introduced to during the pandemic.
Local Knowledge as Illiterate Rhetoric: An Antenarrative Approach to Enacting Socially Just Technical Communication
Authors: Dorpenyo Isidore K.
Published: August 2021

In this article, I focus on two competing technical communication discourses used to represent the biometric technology Ghana adopted in 2012 and subsequent elections to demonstrate how communication about technology could potentially marginalize local, nondominant knowledge systems whereas it privileges global, dominant knowledge systems. Representation of the biometric technology, therefore, reflects ways that technical communication can become complicit in silencing, excluding, and marginalizing local voices. I call attention to how communication that focuses on dominant narratives obscures and delegitimizes the knowledge of disenfranchised and less privileged groups.
“Many are the plans”: An analysis of goals described by youth ministry leaders
Authors: Greenway Tyler S , Powell Kara E , Hanle Lisa E , Jacques Patrick E , Schnitker Sarah A
Published: August 2021

This article examines the content and appraisals of youth ministry leader goals. Responses to an open-ended survey question by 378 leaders primarily working in the United States who held significant responsibility for the discipleship of high school-aged young people in their ministries were coded, resulting in 29 categories of goals. Participants named goals associated with service, relationships in general, relationships with God, biblical/gospel knowledge, and discipleship most frequently. Leaders rated each goal according to factors that contribute to goal achievement and well-being. Appraisals of goal difficulty (“this goal involves challenge”), clarity (“this goal is well-defined”), satisfaction with progress (“this goal is moving forward satisfactorily”), and support (“other leaders and congregants encourage the pursuit of this goal”) varied significantly across goals. Of note, participants rated discipleship and partnerships with parents/families as progressing the least satisfactorily and as some of the most difficult goals. By contrast, participants rated service goals as some of the least difficult and most clear. The content and appraisals of these goals bear implications for both the psychological study of goals and strivings and for ministry practice.
Regional Knowledge Capabilities, Entrepreneurial Activity, and Productivity Growth: Evidence from Italian NUTS-3 Regions
Authors: Kang Taewon , Maliphol Sira , Kogler Dieter F. , Kim Keungoui
Published: August 2021

Knowledge has replaced labor as the key factor for productivity growth in innovation discourse. The Knowledge Spillover Theory of Entrepreneurship (KSTE) provides the theoretical foundation to bridge the gap between knowledge and productivity growth. The way regional knowledge actually contributes to productivity growth requires a theoretical explanation because knowledge capability is an indirect and intangible input for regional productivity growth. Previous research has shown that entrepreneurship alone is insufficient to drive productivity improvements. We examine how knowledge capabilities lead to meaningful growth outcomes of new firms in a region. This study examines the determinants of productivity growth by analyzing the factors of entrepreneurship and knowledge capabilities at the regional level, especially considering the moderating effect of entrepreneurship between knowledge and regional growth; by comparing different dimensions of local knowledge capabilities; and by aggregating the contribution of knowledge capabilities and entrepreneurship to productivity growth at the regional level. The empirical analysis is performed on Italian NUTS-3 regions by utilizing an integrated data set combining patent data from the EPO PATSTAT database, and regional data from Eurostat regional statistics. This study makes two main contributions to the KSTE literature by linking knowledge, entrepreneurship, and regional growth and by providing empirical results on different aspects of regional knowledge capability. Our findings identify which types of local knowledge capabilities are more important and how related innovative activity interacts with entrepreneurial activity, elucidating the mechanisms by which knowledge affects labor productivity through entrepreneurship.