bluuebunny/crossref_metadata_2025 huggingface dataset:
COVID19 Coronavirus Infection in Patients with Acute Myeloid Leukemia: Literature Review and Own Experience
Authors:   GBUZ GKB no 52 DZM, Moscow, Russia, Gagloeva D.E. ,   International Office, State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russiа, Misyurina E.N. ,   GBUZ GKB no 52 DZM, Moscow, Russia, Zhelnova E.I. ,   GBUZ GKB no 52 DZM, Moscow, Russia, Tolstyh T.N. ,   GBUZ GKB no 52 DZM, Moscow, Russia, Rogacheva E.R. ,   International Office, State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russiа, Astrelina T.A. ,   International Office, State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russiа
Published: October 2023

The presented literature review presents studies, including our own, on the peculiarities of the course of coronavirus infection in patients with hematological malignancies, in particular in patients with acute myeloid leukemia (AML). A modern classification of AML is presented. It has been shown that in patients with AML, the addition of coronavirus infection SARS-CoV-2 COVID-19 significantly worsens the prognosis of the patient’s life, which makes significant changes in overall survival and mortality. Factors worsening the prognosis of life in patients with AML are advanced age, comorbidities, AML out of remission, the critical course of SARS-CoV-2 COVID19, interruption of treatment without subsequent resumption. In patients with AML and SARS-CoV-2 COVID19, the most optimal treatment tactic is temporary discontinuation of the course of chemotherapy followed by resumption after the regression of the viral infection (both at the onset of the disease and when chemotherapy is already underway). If it is not possible to postpone the course of chemotherapy, treatment can and should be carried out with the provision of adequate accompanying therapy simultaneously with ethological and pathogenetic therapy for SARS-CoV-2 COVID19. Even in the presence of hematological contraindications in patients with AML, SARS-CoV-2 COVID19 treatment should be carried out in full according to clinical guidelines for the treatment of SARS-CoV-2 COVID19.
Improvement of Information Technologies in the Domestic Agro-Industrial complex
Authors:   FGBNU “Rosinformagrotech”, O.V. Kondratieva
Published: October 2023

Perspective paths of using modern information technologies in the agro-industrial complex are considered. It was revealed that computer programs are the basis of new information technologies, in which advanced methods of agro-industrial production are presented in the form of mathematical models. Examples of the development of digital solutions for enterprises and organizations in the agricultural sector are given.
Features of Standardization of Methods for Economic Evaluation of Machine Technologies for Crop Production
Authors:   KubNIITiM, Sviridova S.A.
Published: October 2023

The features of standardization of methods of economic assessment of machine technologies of plant growing in the developed draft of the interstate standard are given.
Science-based Technology for Growing Potatoes for a Changing Climate
Authors:   Federal State Budgetary Institution “FRC of Potatoes named after A.G. Lorch, Starovoitova O.A. , Starovoitov V.I. ,   Federal State Budgetary Institution “FRC of Potatoes named after A.G. Lorch”, Shabanov N.E. ,   Federal State Budgetary Institution “FRC of Potatoes named after A.G. Lorch”, Manokhina A.A. ,   FSBEI HE “RGAU Moscow Agricultural Academy named after K.A. Timiryazev”
Published: October 2023

An experiment was planned to determine the effectiveness of the influence of technological influences: fertilizers, doses of superabsorbent polymers (SAP) and varieties. The main responses when planning an experiment are the main parameters characterizing the process of growing potatoes: gross yield of tubers, yield of marketable size tubers during harvesting, reduction of storage losses and quality indicators of potato tubers. The results of the experiment are presented in the form of a mathematical model that has certain statistical properties, for example, the minimum variance of estimates of the model parameters.
Substantiation of the Possibility of Diagnosing the Bearing Assembly of a Turbo-compressor by Consumption Characteristics in the MATLAB Environment
Authors:   Moscow State University. N.P. Ogaryov, Kurbakov I.I.
Published: October 2023

The simulation of the process of oil supply of a plain bearing TKR 6.1 was carried out in the Matlab Simulink software package. Values of liquid consumption – oil SAE 30 at a pressure of 0.3 MPa were obtained with a change in its temperature from 30 to 90°С (viscosity from 167.2 to 15.0 cSt) for various values of the total clearances of TKR 6.1, as well as the values of the liquid flow when modeling pressure in the unit from 0.1 to 0.4 MPa and oil temperature 90°C (viscosity 15.0 cSt). The presented results indicate the possibility of using this method to determine the total clearance of the turbocharger bearing assembly when building hardware and software systems for the study, diagnosis and examination of turbochargers and other similar units using the CIP method.
Kajian viabilitas benih sorgum (Sorghum bicolor [L] Moench.) varietas numbu dan UPCA-S2 akibat pengusangan dipercepat maupun periode simpan alami
Authors: Herawati Ni Made , Pramono Eko , Kamal Muhammad
Published: October 2023

Kemunduran benih dapat terjadi akibat lamanya periode penyimpanan atau pengusangan cepat. Viabilitas benih akan lebih rendah karena meningkatnya kemunduran benih. Percobaan ini bertujuan untuk membandingkan viabilitas benih sorgum varietas Numbu dan UPCA-S2, karena periode simpan alami dan karena pengusangan cepat. Intensitas pengusangan cepat adalah meningkatnya konsentrasi larutan etanol. Sebagian benih disimpan di dalam ruangan bersuhu 27,3 ± 0,9 ?C dan viabilitasnya diamati secara berkala setiap dua bulan hingga 6 bulan. Sebagian benih sorgum lainnya diperlakukan dengan melembabkannya dalam larutan etanol dengan peningkatan konsentrasi 0, 4, 8, dan 12%, masing-masing selama 24 jam. Periode simpan alami dan juga perlakuan intensitas pengusangan cepat berpengaruh secara signifikan (P <0,01) pada persentase kecambah normal dan kecepatan perkecambahan. Viabilitas benih kedelai Numbu maupun UPCA-S2 menurun secara signifikan pada perlakuan pengusangan cepat dengan konsentrasi etanol 8%, juga pada perlakuan periode penyimpanan dua bulan.
Seed deterioration can occur by the length of the storage period or accelerated aging. The viability of seeds will be lower due to increasing seed deterioration. This experiment aimed to compare the viability of the seeds of two varieties of sorghum, Numbu, and UPCA-S2, due to the natural storage period and due to accelerated aging.  The intensity of aging was the increasing concentration of ethanol solution. Some seeds were stored in a room with a temperature of  27,3 ± 0,9?C, and their viability was observed periodically every 2 months up to 6 months. Some other sorghum seeds were treated by moisturizing them in ethanol solution with increasing concentrations, 0, 4, 8, and 12%, each for 24 hours. Seed viability was observed at each end of the rapid aging treatment. The storage period and the intensity of aging treatment significantly (P<0,01) reduced the percentage of normal seedling and germination speed.  The viability of Numbu soybean seeds and UPCA-S2 decreased significantly in the accelerated aging treatment with 8% ethanol concentration, also in the two-month storage period treatment.
Analisis kerangka kerja yang logis untuk menyusun sop penanganan pertama pada cctv
Authors: Suratno Danang Mulyadipa , Sutrisno Alya Triska , Novrianti Novrianti
Published: October 2023

Ketika petugas berwenang melakukan aktivitas investigasi forensik digital, hal penting yang harus diperhatikan adalah menggunakan dan mengikuti proses di setiap tahapan yang ada pada kerangka investigasi. Tidak hanya mengikuti mekanisme yang tepat tetapi juga harus mengikuti aturan hukum yang berlaku. Namun ditemukan kejadian saat barang bukti yang dihadirkan dipersidangan ditolak pada perkara nomor: 85/PID.B/2012/PN.pwt, dikarenakan hasil rekaman CCTV tidak disertai alat bukti proses hashing yang dicetak dalam bentuk surat untuk melihat keaslian dari suatu file. Hal ini menunjukkan bahwa pihak pengadilan tidak bisa menerima begitu saja bukti yang diserahkan jika mereka tidak bisa memastikan bagaimana bukti tersebut ditangani. Oleh karena itu, dilakukan penelitian untuk menghasilkan framework penangan awal untuk forensik CCTV melalui identifikasi ketentuan dan proses penting dari standar yang berlaku. Pengamatan pada penelitian ini menggunakan kolaborasi dari dokumen SNI 27037:2014 dan SWGIT 1.0 2013.09.27, sehingga diperoleh tahapan kegiatan terhadap alat bukti digital dimulai dari perolehan hingga diputarkan di pengadilan, dan sesuai dengan prosedur ini maka alat bukti digital tersebut dinyatakan sah di persidangan. Metode teknik analisa yang logis terhadap kerangka kerja yang akan dilakukan (logical framework approach (LFA) dapat diterapkan dalam menyusun sebuah framework forensik untuk penanganan awal forensik barang bukti sistem kamera pengawas CCTV
Time the authorized officer does the digital forensic investigation activity, the important point that surely concern is using and following the process at every step of the investigation framework. Not only following the certain mechanism but also standing by the rule of duty. Unfortunately, there was criminal evidence in the judge ignored at the case with number 85/PID.B/2012/PN.pwt because there was no evidence of hashing proceed tool for CCTV recording that printed as the letter for the originality document of the file. This showed that the people at the judge cannot receive the things for the evidence offhand delivered if they cannot guarantee how that thing is handled. Therefore, this research did in resulting the first response to the framework for CCTV forensics by identifying the rule and important processes from the applied standard. This research used the document SNI 27037:2014 and SWGIT 1.0 2013.09.27, such that yield the recording of CCTV as the legal digital evidence for the judge.
Ekstraksi pektin dari kulit buah pisang cavendish (Musa acumminata Cavendish)
Authors: Nurhayati Anis
Published: October 2023

Senyawa pektin umumnya ada dalam dinding sel utama tumbuhan, terutama di sela-sela antara selulosa dan hemiselulosa, fungsi pektin dalam dinding sel tumbuhan sebagai pengikat antara dinding sel. Industri yang biasanya menggunakan Pektin yaitu industri makanan, minyak dan farmasi. Tujuan dari penelitian ini untuk memanfaatkan limbah kulit pisang cavendish (Musa acumminata Cavendish) sebagai alternative sumber pektin. Metodologi yang digunakan dalam penelitian ini rancangan acak kelompok (RAK) faktorial dengan 2 faktor, normalitas HCl 0,01N, 0,05N, 0,10N dan waktu ekstraksi 60, 90, 120 menit, ekstraksi dilakukan pada suhu 90°C tahap selanjutnya penyaringan, filtrat yang diperoleh didinginkan dan ditambahkan etanol untuk mengendapkan pektin, kemudian dikeringkan untuk mendapatkan pektin kering. Parameter yang diamati; kemurnian pektin, rendemen pektin dan kadar air. Hasil penelitian menunjukkan pektin dengan kemurnian tertinggi 90,8% dari ekstraksi HCl 0,05N dengan waktu 60 menit. Kemurnian pektin terendah 84,07% dari ekstraksi HCl 0,01N dengan waktu 120 menit. Rendemen pektin tertinggi dari ekstraksi HCl 0,05N waktu ekstraksi 90 menit, yaitu 4.57% dan Rendemen pektin terendah dari ekstraksi HCl 0,01 N waktu ekstraksi 60 dan 120 menit, yaitu 3.11 %. Semua perlakuan ekstraksi terdapat interaksi dan berpengaruh nyata terhadap kemurnian pektin dan Rendemen pektin, namun tidak ada pengaruh nyata pada kadar air pektin.
 Pectin compounds are generally present in the primary cell walls of plants, especially on the sidelines between cellulose and hemicellulose, pectin functions in plant cell walls as a binder between cell walls. Pectin is used in the food, oil, and pharmaceutical industries. The purpose of this study was to utilize Cavendish banana peel waste (Musa acumminata Cavendish) as an alternative source of pectin. The methodology used in this study was factorial randomized block design (RAK) with 2 factors, normality of HCl 0.01N, 0.05N, 0.10N and extraction time of 60.90, 120 minutes, the extraction was carried out at a temperature of 90°C, the next stage was filtering, the filtrate obtained is cooled, and ethanol is added to precipitate the pectin, then dried to obtain dry pectin. Parameters observed; pectin purity, pectin yield, and water content. The results showed pectin with the highest purity, 90.8%, from 0.05N HCl extraction with a time of 60 minutes. The lowest pectin purity was 84.07% from 0.01N HCl extraction within 120 minutes. The highest pectin yield from 0.05 N HCl extraction with 90 minutes extraction time, which is 4.57%, and the lowest pectin yield from 0.01 N HCl extraction with 60 and 120 minutes extraction time, which is 3.11%. All extraction treatments had an interaction and significant effect on pectin purity and pectin yield, but there was no significant effect on pectin moisture content.
A new species of Chalcis Fabricius (Hymenoptera: Chalcididae) from south India
Authors: Ranjith A.P. , Priyadarsanan Rajan
Published: October 2023

The morphologically diverse and cosmopolitan chalcidid genus, Chalcis includes 59 species. Most of the species are distributed in the temperate regions of the Northern Hemisphere. Till date only two species were recorded from the Oriental region. A new species, Chalcis biligiriensis from scrub forests of Biligiri Rangaswamy Temple Tiger Reserve (BRT) of the Western Ghats (Karnataka, India) is described. In addition, detailed illustrations of C. gibsoni Narendran for the first time along with an illustrated key to the identification of the Oriental species are provided.
La aplicación de técnicas no invasivas para la documentación de la arquitectura doméstica italicense
Authors: Corrales Álvarez Álvaro , Velázquez Guerrero Maite , Hermann Florian , Teichner Félix , Hidalgo Prieto Rafael
Published: October 2023

El área de Arqueología de la Universidad Pablo de Olavide, en colaboración con la Universidad de Marburgo, lleva a cabo en la actualidad una actividad arqueológica puntual de prospección geofísica y documentación de las insulae de Italica (Santiponce, Sevilla). El objetivo del proyecto es desarrollar una nueva documentación gráfica de la arquitectura doméstica italicense a partir de metodologías de teledetección y geofísica superficial. Esta propuesta incluye el uso de equipamiento científico de última generación con medidas no invasivas con la utilización de escáner láser terrestre, dron, así como magnetómetro, georradar y resistivímetro. Cada una de las metodologías se aplicará en diferentes espacios domésticos con el fin de optimizar los resultados. El uso del escáner láser terrestre se aplicará a la arquitectura residencial visible en el Conjunto Arqueológico de Itálica. Por el contrario, el empleo de equipamiento geofísico (magnético, radar y resistividad) se circunscribe a las manzanas cuyo uso de suelo, de probable carácter residencial, no han sido probado todavía. Si bien, se utilizará su uso también para resolver dudas puntuales sobre la morfología de algunas manzanas residenciales no excavadas como la parcela aledaña a la Casa del Planetario. El proyecto planteado viene a completar el estudio de la arquitectura doméstica de Itálica, dotando al yacimiento de una base científica con todas las garantías que la permitan constituirse en un caso de referencia, para esta temática, en el conjunto del Imperio Romano.
NanoCult: nanosoluciones avanzadas para la consolidación y protección multifuncional del patrimonio cultural
Authors: Costantini Ilaria , Etxebarria Idoia , Vázquez de la Fuente Iñaki , Aramendia Julene , Arana Gorka , Prieto Nagore , Castro Kepa , Martinez-Arkarazo Irantzu , Sanchez Iban , Azkarate Agustín , Sanz Macarena , Pérez Lucía , Yedra Ángel , Fernández Acevedo Claudio , Yécora Beatriz , Oroz Tamara , Madariaga Juan Manuel
Published: October 2023

Cuatro entidades nacionales, la Universidad del País Vasco (UPV/EHU), el Centro Tecnológico Lurederra de Navarra, la empresa de restauración Garanza (Madrid) y el Centro Tecnológico CTC de Cantabria, colaboran en este proyecto de carácter multidisciplinar que aborda la conservación del patrimonio cultural a través del desarrollo de innovadoras metodologías de diagnóstico, soluciones de limpieza, consolidación y protección no invasivas basadas en la nanotecnología. El proyecto, que se ensaya en pinturas murales de la localidad de Navalcarnero y en los muros exteriores de la catedral de Santa María y de la muralla de Vitoria Gasteiz, comenzó en noviembre de 2021 y tiene un periodo de ejecución de 36 meses.
Rituals Within Walls: Thinking Post-War Japan’s History through Cinematic Allegories of Everyday Life
Authors: de Vargas Ferran
Published: October 2023

Between the mid-1960s and the early 1970s, the quotidian dimension took political centrality in Japan thanks to the leading role of the New Left movement and its ideology. This went hand in hand with an appreciation of the philosophical approaches of Marxist intellectuals such as Jun Tosaka and Gorō Hani, who saw the quotidian as a fundamental space for historical transformation. We know how Tosaka and Hani developed an everyday-centred philosophy of history through their writings, but we know little about how formats other than the written word – such as cinema – contributed to thinking historical reality from the same ideological approach. To address this gap in our understanding, this article analyses the intersection of two highly representative films with Tosaka and Hani’s historical thought: Secrets Within Walls ( Kabe no naka no himegoto, Kōji Wakamatsu, 1965) and Ceremonies ( Gishiki, Nagisa Ōshima, 1971). These films’ cinematic allegorisation of post-war Japan’s everyday relationships was a way to reflect on the political character of that period.
The Monstrous Mark of Cinema: Mulholland Drive, Spherology, and the “Virtual Space” of Filmic Fiction
Authors: Dutton James
Published: October 2023

This article interprets David Lynch's Mulholland Drive (2001) to argue for the morphological influence cinematic images have on modernity's monstrous identity. It shows how Lynch's tactic of interweaving apparently discrete spaces of dream and reality – one often inverting or uncannily ironising the other – relies on the virtual space of cinema, which leaves a mark on understanding, irrespective of its apparent truth. To do so, I employ Peter Sloterdijk's philosophy of space – especially the spherology developed in his Spheres trilogy – and Bernard Stiegler's approach to cinematic phenomenology to consider the transferential force of cinema, and its power to cultivate and shape popular consciousness. Mulholland Drive is acutely aware of this force, and its interplay with the expectations and disappointments of Hollywood monstrosity inserts itself into the sphere-forming traffic of modern meaning-making. In doing so, I argue, the film takes up Lynch's familiar approach to irony and the weird to unwind the destructive uniformity of Hollywood hegemony. The productive bifurcations that result, inscribed in between cinema and consciousness, and forgettable as the very spheric intimacy of memory, gesture to what might live beyond discrete forms.
AN EVALUATION OF DIGITAL GOVERNANCE VISION AND CURRENT PRACTICES WITHIN THE FRAMEWORK OF PARTICIPATORY MANAGEMENT ON THE OCCASION OF THE 100TH ANNIVERSARY OF THE REPUBLIC
Authors: YIKILMAZ İbrahim
Published: October 2023

The Great Turkish Nation fought for many years and struggled for Independence. With Mustafa Kemal ATATÜRK's declaration of the Republic, a form of government in which sovereignty rests unconditionally on the Turkish Nation, the nation gained a more democratic and freedom-based regime. Mustafa Kemal ATATÜRK emphasized that the Turkish Nation should always be involved in participatory management, both through parliament and civil initiatives, to protect the Republic regime and its future. Since the founding of the Republic, the developments in information and communication technologies have significantly changed public service delivery and government-citizen interaction. To develop participatory management practices, the digital governance vision comes to the fore, in which digital opportunities increase public service delivery and citizen integration. Digital governance is a more democratic and citizen-oriented management approach. It allows citizen-government and private enterprises to act with a common initiative to solve social problems. On the 100th anniversary of the Republic, the concept of digital governance is examined to increase the effectiveness of the regime based on popular will and participatory management. In accordance with this purpose, information, and evaluations about existing e-government and e-municipality applications are shared. The study is expected to increase awareness of the concept of digital governance among policymakers and citizens, thereby contributing to the development of the participatory management approach and public management effectiveness.
EMPREENDEDORISMO NEGRO E BLACK MONEY
Authors: Zanella Nathalia , Magalhães Yana
Published: October 2023

O empreendedorismo negro, ou afroempreendedorismo, pode ser considerado uma forma de empreender que se relaciona com o dinheiro que circula entre a comunidade negra, o “black money”. O objetivo geral deste estudo foi analisar a expansão do empreendedorismo negro no Rio de Janeiro, considerando o crescimento dos empreendimentos negros nos últimos cinco anos sob o ponto de vista dos(as) empreendedores(as) envolvidos(as); compreendendo o conhecimento dos(as) empreendedores(as) negros(as) do Rio de Janeiro acerca do black money e sua contribuição para a expansão do empreendedorismo negro no Estado; e mapeando os eventos propícios a atividades de empreendedores(as) negros(as) no Rio de Janeiro e sua importância segundo os(as) próprios(as) empreendedores(as).
Physiological oxygen measurements in vitro-Schrödinger’s cat in 3D cell biology
Authors: Gottwald Eric , Grün Christoph , Nies Cordula , Liebsch Gregor
Published: October 2023

After the development of 3D cell culture methods in the middle of the last century and the plethora of data generated with this culture configuration up to date, it could be shown that a three-dimensional arrangement of cells in most of the cases leads to a more physiological behavior of the generated tissue. However, a major determinant for an organotypic function, namely, the dissolved oxygen concentration in the used in vitro-system, has been neglected in most of the studies. This is due to the fact that the oxygen measurement in the beginning was simply not feasible and, if so, disturbed the measurement and/or the in vitro-system itself. This is especially true for the meanwhile more widespread use of 3D culture systems. Therefore, the tissues analyzed by these techniques can be considered as the Schrödinger’s cat in 3D cell biology. In this perspective paper we will outline how the measurement and, moreover, the regulation of the dissolved oxygen concentration in vitro-3D culture systems could be established at all and how it may be possible to determine the oxygen concentration in organoid cultures and the respiratory capacity via mito stress tests, especially in spheroids in the size range of a few hundred micrometers, under physiological culture conditions, without disturbances or stress induction in the system and in a high-throughput fashion. By this, such systems will help to more efficiently translate tissue engineering approaches into new in vitro-platforms for fundamental and applied research as well as preclinical safety testing and clinical applications.
In vivo characterization of 3D-printed polycaprolactone-hydroxyapatite scaffolds with Voronoi design to advance the concept of scaffold-guided bone regeneration
Authors: Laubach Markus , Herath Buddhi , Bock Nathalie , Suresh Sinduja , Saifzadeh Siamak , Dargaville Bronwin L. , McGovern Jacqui , Wille Marie-Luise , Hutmacher Dietmar W. , Medeiros Savi Flavia
Published: October 2023

Graphical Abstract

partially created with BioRender.com.
Response mechanisms to acid stress promote LF82 replication in macrophages
Authors: Yao Ting , Huang Yu , Huai Zimeng , Liu Xingmei , Liu Xiaowen , Liu Yutao , Sun Hao , Pang Yu
Published: October 2023

Background

Adherent–invasive E. coli (AIEC) LF82 is capable of adhering to and invading intestinal epithelial cells, as well as replicating within macrophages without inducing host cell death.

Methods

We compared the transcriptomics of LF82 at pH=7.5 and pH=5.8 by RNA-sequencing, and qRT-PCR verified differentially expressed genes (DEGs). The deletion mutants of DEGs in the treatment group (pH=5.8) compared to the control group (pH=7.5) were constructed by λ recombinant. The replication differences between the mutants and WT infected Raw 264.7 at 24 h.p.i were analyzed by combining LB solid plate count and confocal observation. NH4Cl and chloroquine diphosphate (CQ) were used for acid neutralization to study the effect of pH on the replication of LF82 in macrophages. Na2NO3 was added to RPMI 1640 to study the effect of nitrate on the replication of LF82 in macrophages. 0.3% solid LB was used for flagellar motility assay and Hela was used to study flagellar gene deletion mutants and WT adhesion and invasion ability.

Results

In this study, we found that infection with LF82 results in acidification of macrophages. Subsequent experiments demonstrated that an intracellular acidic environment is necessary for LF82 replication. Transcriptome and phenotypic analysis showed that high expression of acid shock genes and acid fitness genes promotes LF82 replication in macrophages. Further, we found that the replication of LF82 in macrophages was increased under nitrate treatment, and nitrogen metabolism genes of LF82 were upregulated in acid treatment. The replication in macrophages of ΔnarK, ΔnarXL, ΔnarP, and Δhmp were decreased. In addition, we found that the expression of flagellar genes was downregulated in acidic pH and after LF82 invading macrophages. Motility assay shows that the movement of LF82 on an acidic semisolid agar plate was limited. Further results showed that ΔfliC and ΔfliD decreased in motility, adhesion ability, and invasion of host cells, but no significant effect on replication in macrophages was observed.

Conclusion

In this study, we simulated the acidic environment in macrophages, combined with transcriptome technology, and explained from the genetic level that LF82 promotes replication by activating its acid shock and fitness system, enhancing nitrate utilization, and inhibiting flagellar function.
Sensor histidine kinases kdpD and aauS regulate biofilm and virulence in Pseudomonas aeruginosa PA14
Authors: Sultan Maria , Arya Rekha , Chaurasia Akhilesh Kumar , Kim Kyeong Kyu
Published: October 2023

Pseudomonas aeruginosa is a multidrug-resistant opportunistic human pathogen that utilizes two-component systems (TCSs) to sense pathophysiological signals and coordinate virulence. P. aeruginosa contains 64 sensor histidine kinases (HKs) and 72 response regulators (RRs) that play important roles in metabolism, bacterial physiology, and virulence. However, the role of some TCSs in virulence remains uncharacterized. In this study, we evaluated the virulence potential of some uncharacterized sensor HK and RR knockouts in P. aeruginosa using a Galleria mellonella infection model. Furthermore, we demonstrated that KdpD and AauS HKs regulate virulence by affecting P. aeruginosa biofilm formation and motility. Both ΔkdpD and ΔaauS showed reduced biofilm and motility which were confirmed by restored phenotypes upon complementation. Moreover, ΔkdpD and ΔaauS exhibited increased survival of HeLa cells and G. mellonella during in vivo infection. Altered expression of the transcriptional regulators anR and lasR, along with the virulence genes lasA, pelA, cupA, pqsA, pqsB, pqsC, and pqsD in the mutant strains elucidated the mechanism by which ΔkdpD and ΔaauS affect virulence. These findings confirm that kdpD and aauS play important roles in P. aeruginosa pathogenesis by regulating biofilm formation and motility.
Age and task type effects on comprehension and production of narrative macrostructure: storytelling and retelling by Swedish-speaking children aged 6 and 8
Authors: Lindgren Josefin
Published: October 2023

It is often said that story retelling tasks, where children listen to a model story and then retell it, are easier than story telling tasks, where children are not provided with a model. However, previous studies have rarely used comparable stimuli and procedures for the different tasks, creating possible confounds with task effects. Additionally, studies seldom investigate the interaction between age and task type and most studies focus on preschool children. The present study addresses these gaps by analyzing the performance of Swedish-speaking 6-year-olds and 8-year-olds (N = 74) on measures of story comprehension and story structure (narrative macrostructure) using a carefully controlled procedure with comparable telling and retelling tasks (MAIN Cat/Dog stories) and counterbalancing the order of the tasks. For story comprehension, results showed that overall accuracy was uniformly high (>90%) across tasks and age groups. However, performance was substantially lower for one question (D10), which assesses comprehension of the entire plotline. With increasing age, children did not become more likely to answer this question correctly, nor did hearing a model story improve performance. A qualitative analysis showed that incorrect answers often contained reasonable explanations showing advanced general inferencing abilities. In light of these results, an adjustment to the scoring of MAIN is recommended. For story structure, results showed significant effects of both age and task type, with higher scores in retelling and higher scores by the 8-year-olds. The 8-year-olds exhibited the same performance gap between telling and retelling as the 6-year-olds. There was also a significant effect of task order, showing a training effect from the first task to the second. The present study thus confirms previous findings that expressing story structure in a retelling task is easier than in a telling task, but showing this for the first time while controlling for task order and stimulus complexity in MAIN.
Left ventricular dyssynchrony measured by cardiovascular magnetic resonance-feature tracking in anterior ST-elevation myocardial infarction: relationship with microvascular occlusion myocardial damage
Authors: Sun Zheng , Wang Yu , Hu Yingying , Wu Fang , Zhang Nan , Liu Zhi , Lu Jie , Li Kuncheng
Published: October 2023

Objectives

Cardiovascular magnetic resonance-feature tracking (CMR-FT) enables quantification of myocardial deformation and may be used as an objective measure of myocardial involvement in ST-elevation myocardial infarction (STEMI). We sought to investigate the associations between myocardial dyssynchrony parameters and myocardium damage for STEMI.

Methods

We analyzed 65 patients (45–80 years old) with anterior STEMI after primary percutaneous coronary intervention during 3–7 days [observational (STEMI) group] and 60 healthy volunteers [normal control (NC) group]. Myocardial dyssynchrony parameters were derived, including global and regional strain, radial rebound stretch and displacement, systolic septal time delay, and circumferential stretch.

Results

CMR characteristics, including morphologic parameters such as left ventricular ejection fraction (LVEF) (45.3% ± 8.2%) and myocardium damage in late gadolinium enhancement (LGE) (19.4% ± 4.7% LV), were assessed in the observation group. The global radial strain (GRS) and global longitudinal strain (GLS) substantially decreased in anterior STEMI compared with the NC group (GRS: 19.4% ± 5.1% vs. 24.8% ± 4.0%, P < 0.05; GLS: −10.1% ± 1.7% vs. −13.7% ± 1.0%, P < 0.05). Among 362 infarcted segments, radial and circumferential peak strains of the infarcted zone were the lowest (14.4% ± 3.2% and −10.7% ± 1.6%, respectively). The radial peak displacement of the infarct zone significantly decreased (2.6 ± 0.4 mm) (P < 0.001) and manifested in the circumferential displacement (3.5° ± 0.7°) in the STEMI group (P < 0.01). As microvascular occlusion (MVO) was additionally present, some strain parameters were significantly impaired in LGE+/MVO+ segments (radial strain [RS]: 12.2% ± 2.1%, circumferential strain [CS]: −9.6% ± 0.7%, longitudinal strain [LS]: −6.8% ± 1.0%) compared to LGE+/MVO− (RS: 14.6% ± 3.2%, CS: −10.8% ± 1.8%, LS: −9.2% ± 1.3%) (P < 0.05). When the extent of transmural myocardial infarction is greater than 75%, the parameter of the systolic septal delay (mean, 148 ms) was significantly reduced compared to fewer degrees of infarction (P < 0.01).

Conclusion

In anterior STEMI, the infarcted septum swings in a bimodal mode, and myocardial injury reduces the radial strain contractility. A more than 75% transmural degree was the septal strain-contraction reserve cut-off point.
Sex differences in readmission rate after cardiac surgery
Authors: Koechlin Luca , Miazza Jules , Gahl Brigitta , Santer David , Vöhringer Luise , Berdajs Denis , Eckstein Friedrich S. , Reuthebuch Oliver
Published: October 2023

Introduction

The impact of sex on hospital readmission rate after cardiac surgery is unclear. Therefore, we aimed to analyse sex-specific differences and underlying factors in 30-day readmission rate after cardiac surgery.

Methods

We conducted a single center study including all patients after major cardiac surgery (excluding aortic dissection and left ventricular assist device implantation) from January 2012 to September 2020. Reasons for readmission were adjudicated according to all available medical records. We calculated incidence rate ratios (IRR) with 95% confidence intervals (CI) for female sex with re-admission crude and adjusted for plausible confounding factors using negative binomial regression.

Results

4,868 patients were included in the analysis. The median [Interquartile range] age was 68 [60 to 74] years and 24% (n = 1,149) of the patients were female. Female patients were significantly older (median [IQR] age 70 (63 to 76) vs. 67 (59 to 74), p < 0.001) and had lower body mass index and fewer cardiovascular risk factors compared to men. Isolated valve surgery was more frequent in female while coronary artery bypass grafting was more often in men. 30-day readmission was comparable between both sexes (7.0% [n = 81] in female vs. 8.7% [n = 322] in men; p = 0.078). Cardiac related readmissions and infections were the most common reasons for readmission in both groups. The overall incidence rate ratios of female sex with readmission (0.80, 95% CI 0.63 to 1.03, p = 0.078) remained robust after adjustment for EuroSCORE 2 (0.78, CI 0.61 to 1.0, p = 0.051).

Conclusion

Readmission rate and reasons for 30-day readmission after major cardiac surgery were similar between men and women.
Climbing crags recommender system in Arco, Italy: a comparative study
Authors: Ivanova Iustina , Wald Mike
Published: October 2023

Outdoor sport climbing is popular in Northern Italy due to its vast amount of rock climbing places (such as crags). New climbing crags appear yearly, creating an information overload problem for tourists who plan their sport climbing vacation. Recommender systems partly addressed this issue by suggesting climbing crags according to the most visited places or the number of suitable climbing routes. Unfortunately, these methods do not consider contextual information. However, in sport climbing, as in other outdoor activities, the possibility of visiting certain places depends on several contextual factors, for instance, a suitable season (winter/summer), parking space availability if traveling with a car, or the possibility of climbing with children if traveling with children. To address this limitation, we collected and analyzed the crag visits in Arco (Italy) from an online guidebook. We found that climbing contextual information, similar to users' content preferences, can be modeled by a correlation between recorded visits and crags features. Based on that, we developed and evaluated a novel context-aware climbing crags recommender system Visit & Climb, which consists of three stages as follows: (1) contextual information and content tastes are learned automatically from the users' logs by computing correlation between users' visits and crags' features; (2) those learned tastes are further made adjustable in a preference elicitation web interface; (3) the user receives recommendations on the map according to the number of visits made by a climber with similar learned tastes. To measure the quality of this system, we performed an offline evaluation (where we calculated Mean Average Precision, Recall, and Normalized Discounted Cumulative Gain for top-N), a formative study, and an online evaluation (in a within-subject design with experienced outdoor climbers N = 40, who tried three similar systems including Visit & Climb). Offline tests showed that the proposed system suggests crags to climbers accurately as the other classical models for top-N recommendations. Meanwhile, online tests indicated that the system provides a significantly higher level of information sufficiency than other systems in this domain. The overall results demonstrated that the developed system provides recommendations according to the users' requirements, and incorporating contextual information and crag characteristics into the climbing recommender system leads to increased information sufficiency caused by transparency, which improves satisfaction and use intention.
Covering digital health applications in the public insurance system: how to foster innovation in patient care while mitigating financial risks—evidence from Germany
Authors: Groene Nicole , Schneck Luca
Published: October 2023

Context

Digital health applications that support patients in managing their condition can have a positive impact on patients' health and improve the overall care process. In late 2019, as the first country worldwide, Germany included digital health applications in the benefit basket of the statutory health insurance (SHI) system to enable fast, broad-scale patient access and encourage innovation in the digital health industry. While the policy is widely recognized as a pioneering step toward improving patient care through digital technologies, there are concerns regarding the mechanics of the policy and the resulting financial risks for the SHI system.

Goals

The primary objective of this article is to provide a comprehensive and balanced overview of the German policy by evaluating its success in achieving its goals and by reviewing challenges that have emerged. The secondary objective is to delineate prospective policy options and areas warranting future research.

Approach

The article analyzes publicly available data of the Federal Institute for Drugs and Medical Devices collected between February 1st and July 17th, 2023, and complements it with empirical findings published by academic institutions and sickness funds. It discusses policy options and related areas of future research to overcome the identified challenges without jeopardizing the purpose of the legislation to encourage innovation in the digital health industry to improve patient care.

Conclusion

In line with the goals of the reimbursement policy, the inclusion of digital health applications in the SHI benefit basked has entailed new digital treatment options for patients across multiple disease areas. However, from a health policy perspective, the policy has several shortcomings, including low prescription rates, the temporary reimbursement of digital health applications that lack proven benefit, and a pricing framework that does not take into account the efficacy and efficiency of a treatment and may lead to a suboptimal allocation of public resources. Rather than the public system covering digital health applications without proven benefit, the authors suggest giving SHI organizations more budget authority to directly incentivize research and development activities and to introduce value-based pricing. More research is needed to determine the details of these mechanisms.
Dimensions of bilingual education program implementation in schools serving linguistically minoritized students in the Czech-Polish border region
Authors: Hopkins Megan , Nowak-Żółty Edyta , Kurowska-Pysz Joanna , Szyszka Michał
Published: October 2023

Introduction

A core challenge for the implementation of bilingual programs is ensuring that linguistically minoritized students have equitable access to instruction that honors and values their linguistic and cultural assets and strengths. To understand how leaders of bilingual programs might address this challenge, this study sought to identify dimensions of program implementation in bilingual schools serving Polish minorities in the Zaolzie region on the Czech Republic side of the Czech-Polish border.

Methods

Employing a sequential explanatory mixed methods design, the research team first collected surveys from 123 teachers and used factor analytical techniques to identify key dimensions of program implementation. Then, interview and observational data were collected and analyzed using deductive coding to develop descriptions of each dimension in this specific border region.

Results

The analysis revealed four dimensions of program implementation: (a) program structure and alignment; (b) culturally and linguistically responsive instruction; (c) staff quality and professional learning; and (d) family engagement and community support. They also showed how a network of community-based organizations partnered with schools and families in ways that supported each dimension and facilitated integration across dimensions.

Discussion

The findings have several implications for leaders and researchers in bilingual schools including the important role that community organizations and binational cooperation can play in positively contributing to the education of linguistically minoritized populations.
Solution-processed ZnO coated on LiNi0.8Mn0.1Co0.1O2(NMC811) for enhanced performance of Li-ion battery cathode
Authors: Kang Chiwon , Park Yewon , Kim Yongjoon , Kim Soo Min , Ha Seungho , Yoon Hee Gon , Oh Kyu Won , Shin Keun-Young , Kim Byung Hyo
Published: October 2023

The LiNi0.8Mn0.1Co0.1O2 (NMC811) cathode material, widely used in Li-ion batteries (LIBs) for electric vehicles (EVs), has gained a fair amount of attention in the industry due to its advantages of high energy capacity and low production cost. However, during charge-discharge cycles, NMC811 cathode faces issues such as hydrofluoric acid (HF) attack, leaching of transition metals and unstable formation of the cathode electrolyte interphase (CEI), which leads to undermining cathode performance. To address these issues, extensive research has been conducted on coating materials based on metal oxides. In this study, our research team chose Li-doped ZnO (LZO) material, known for its high Li+ ion conductivity and structural stability. Employing sol-gel synthetic method, we successfully coated LZO material on commercial NMC811 particles, therefore ensuring its coating uniformity through X-ray photoelectron spectroscopy (XPS) and energy-dispersive X-ray spectroscopy energy dispersive spectroscopy analyses. Evaluation of the coated samples (1 wt%, 2 wt%, and 3 wt% LZO on NMC811) revealed their superior electrochemical performance compared to bare NMC811; furthermore, the 2 wt% LZO-coated sample exhibited the highest cycling performance among the coated samples. These findings could be attributed to the lower charge transfer resistance verified by electrochemical impedance spectroscopy (EIS) analysis. Thus, we confirmed the LZO coating layers could provide stability for the NMC811 surface structure, mitigate the leaching of transition metal ions in the NMC811, and promote the formation of an enhanced CEI layer, therefore facilitating Li+ ion diffusion.
Design impacts of citizen science. A comparative analysis of water monitoring projects
Authors: Kirschke Sabrina , Bennett Christy , Ghazani Armin Bigham , Kirschke Dieter , Lee Yeongju , Loghmani Khouzani Seyed Taha , Nath Shuvojit
Published: October 2023

Citizen science is often promoted as having the capacity to enable change–from increasing data provision and knowledge product development, via behavioral change of citizens, to problem-solving. Likewise, researchers increasingly emphasize the role of project design in initiating these changes through citizen science. However, respective claims are mostly based on single case studies and reviews, calling for a systematic comparative approach to understanding the effects of project design on change. Based on a survey of 85 water-related citizen science projects from 27 countries, we analyze the comparative effects of literature-based design principles on project impacts. Factor analysis first reveals three key impact factors which are ‘Data output’, ‘Citizen outcome’, and ‘Impact chain’. Regression analysis then shows that these impact factors are significantly influenced by several design factors, amongst which motivational factors are most prominent. The analysis also shows that design factors are most important for ‘Impact chain’, followed by ‘Citizen outcome’, and ‘Data output’. While design factors only partly explain the overall project effects, the regression results are rather stable and significant when including other potential influencing factors like project responsibility and funding. In sum, the results provide an empirically substantiated and differentiated understanding of citizen science impacts and how these are influenced by project design.
Effects of various land utilization types on groundwater at different temporal scales: a case study of Huocheng plain, Xinjiang, China
Authors: Lu Yongxing , Dai Liangliang , Yan Guanxi , Huo Zhitao , Chen Wen , Lan Jingzhou , Zhang Chaoming , Xu Qingyang , Deng Shimin , Chen Jian
Published: October 2023

Different land utilization types have unignorable impacts on adjacent aquifers, so studying the effects of varying land utilization types on groundwater balance and groundwater table in arid and semi-arid areas is crucial to facilitate the rational development of territorial space and groundwater resource management. This study investigated the relationship between land utilization type variations and groundwater dynamics from spatial and temporal scales in Huocheng plain, Xinjiang, China, via taking advantage of different land utilization maps provided by remote sensing techniques in 1990, 2000, and 2015. Based on the analysis of results, the findings can be summarized as follows. First, the cultivated and construction land has expanded significantly in the past 25 years. The migration of the regional center of cultivated land was insignificant, as it is still located almost in the center of the entire study area. However, the transfer span of the location center of construction land was more prominent, and its moving direction was mainly from south to northeast, directly affecting the groundwater balance state and groundwater table. Second, the total amount of groundwater storage exhibited a remarkable reduction, from a recharge in 1990 to a continuous discharge in 2000–2015, during which the total recharge and discharge had both continuously declined. Meanwhile, recharging and discharging elements were also changed profoundly as less groundwater recharges from ambient aqueous environments and more groundwater extraction. Thus, human activities were the fundamental driving terms causing the changes in groundwater sources. Third, the groundwater table gradually and continuously dropped from south to northeast in the study area over time. The drawdown of spatially averaged groundwater tables was positively correlated with construction and cultivated area extension. In addition, compared to the former literature, this study offers an alternative approach for groundwater protection and optimization of territorial space development and utilization in arid and semi-arid areas.