bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Fire Impact of Cellulose-Based Indoor Building Finishing Materials According to Changes in Room Size Aspect Ratio
Authors: Kim Yongjoo , Rie Dongho
Published: June 2024

In modern society, the size of buildings tends to expand due to technological advances. However, while prioritizing performance design and specific building materials, fire research has fallen short of creating a comprehensive fire characteristic database for building materials. This study presents a plan for building a big data resource to evaluate the fire performance of cellulose-based flame retardant building finishing material in buildings of varying sizes. The three types of building finishing materials applied in this study are polyurethane foam, MDF, and cellulose-based building finishing materials. The variables were determined based on the floor area, and the change in floor area was calculated using the aspect ratio, a dimensionless number. Data analysis utilized the Fire Dynamics Simulator (FDS) to determine the time required to meet life safety standards for temperature, visibility, and Fractional Effective Dose (FED). The results confirm a correlation between the safe evacuation time (ASET) and increasing floor area. Additionally, the study demonstrates that cellulose-based flame-retardant building finishing material effectively maintains safe evacuation times even with increasing floor areas, as evidenced by increases of 41.0 s, 13.2 s, and 97.5 s in temperature, visibility, and FED, respectively.
Seasonal Variations in Radon and Thoron Exhalation Rates from Solid Concrete Interior Walls Observed Using In Situ Measurements
Authors: Sakoda Akihiro , Ishimori Yuu , Hasan Md. Mahamudul , Jin Qianhao , Iimoto Takeshi
Published: June 2024

Building materials, such as brick and concrete, are known indoor radon (222Rn) and thoron (220Rn) sources. Most radon and thoron exhalation studies are based on the laboratory testing of pieces and blocks of such materials. To discuss if laboratory findings can be applied to a real-world environment, we conducted intensive in situ exhalation tests on two solid concrete interior walls of an apartment in Japan for over a year. Exhalation rates of radon (JRn) and thoron (JTn) were measured using an accumulation chamber and dedicated monitors, alongside monitoring indoor air temperature (T) and absolute humidity (AHin). There were weak correlations between JRn or JTn and T or AHin at one tested wall, and moderate correlations of JRn and strong correlations of JTn with T or AHin at the other wall, meaning more or less seasonal variations. The findings aligned with previous laboratory experiments on JRn but lacked corresponding data for JTn. Additionally, a moderate or strong correlation between JRn and JTn was observed for both tested walls. Comparison with theoretical calculations revealed a new issue regarding the impact of each process of emanation and migration within concrete pores on radon and thoron exhalation. Overall, this study provides insight into parameterizing radon and thoron source inputs in modeling the spatiotemporal dynamics of indoor radon and thoron.
Diverse Shape Design and Physical Property Evaluation of In-Body Tissue Architecture-Induced Tissues
Authors: Tajikawa Tsutomu , Sekido Yota , Mori Kazuki , Kawashima Takayuki , Nakashima Yumiko , Miyamoto Shinji , Nakayama Yasuhide
Published: June 2024

Autologous-engineered artificial tissues constitute an ideal alternative for radical surgery in terms of natural anticoagulation, self-repair, tissue regeneration, and the possibility of growth. Previously, we focused on the development and practical application of artificial tissues using “in-body tissue architecture (iBTA)”, a technique that uses living bodies as bioreactors. This study aimed to further develop iBTA by fabricating tissues with diverse shapes and evaluating their physical properties. Although the breaking strength increased with tissue thickness, the nominal breaking stress increased with thinner tissues. By carving narrow grooves on the outer periphery of an inner core with narrow grooves, we fabricated approximately 2.2 m long cord-shaped tissues and net-shaped tissues with various designs. By assembling the two inner cores inside the branched stainless-steel pipes, a large graft with branching was successfully fabricated, and its aortic arch replacement was conducted in a donor goat without causing damage. In conclusion, by applying iBTA technology, we have made it possible, for the first time, to create tissues of various shapes and designs that are difficult using existing tissue-engineering techniques. Thicker iBTA-induced tissues exhibited higher rupture strength; however, rupture stress was inversely proportional to thickness. These findings broaden the range of iBTA-induced tissue applications.
Advancing DNA Language Models through Motif-Oriented Pre-Training with MoDNA
Authors: An Weizhi , Guo Yuzhi , Bian Yatao , Ma Hehuan , Yang Jinyu , Li Chunyuan , Huang Junzhou
Published: June 2024

Acquiring meaningful representations of gene expression is essential for the accurate prediction of downstream regulatory tasks, such as identifying promoters and transcription factor binding sites. However, the current dependency on supervised learning, constrained by the limited availability of labeled genomic data, impedes the ability to develop robust predictive models with broad generalization capabilities. In response, recent advancements have pivoted towards the application of self-supervised training for DNA sequence modeling, enabling the adaptation of pre-trained genomic representations to a variety of downstream tasks. Departing from the straightforward application of masked language learning techniques to DNA sequences, approaches such as MoDNA enrich genome language modeling with prior biological knowledge. In this study, we advance DNA language models by utilizing the Motif-oriented DNA (MoDNA) pre-training framework, which is established for self-supervised learning at the pre-training stage and is flexible enough for application across different downstream tasks. MoDNA distinguishes itself by efficiently learning semantic-level genomic representations from an extensive corpus of unlabeled genome data, offering a significant improvement in computational efficiency over previous approaches. The framework is pre-trained on a comprehensive human genome dataset and fine-tuned for targeted downstream tasks. Our enhanced analysis and evaluation in promoter prediction and transcription factor binding site prediction have further validated MoDNA’s exceptional capabilities, emphasizing its contribution to advancements in genomic predictive modeling.
Novel Methods for Personalized Gait Assistance: Three-Dimensional Trajectory Prediction Based on Regression and LSTM Models
Authors: Romero-Sorozábal Pablo , Delgado-Oleas Gabriel , Laudanski Annemarie F. , Gutiérrez Álvaro , Rocon Eduardo
Published: June 2024

Enhancing human–robot interaction has been a primary focus in robotic gait assistance, with a thorough understanding of human motion being crucial for personalizing gait assistance. Traditional gait trajectory references from Clinical Gait Analysis (CGA) face limitations due to their inability to account for individual variability. Recent advancements in gait pattern generators, integrating regression models and Artificial Neural Network (ANN) techniques, have aimed at providing more personalized and dynamically adaptable solutions. This article introduces a novel approach that expands regression and ANN applications beyond mere angular estimations to include three-dimensional spatial predictions. Unlike previous methods, our approach provides comprehensive spatial trajectories for hip, knee and ankle tailored to individual kinematics, significantly enhancing end-effector rehabilitation robotic devices. Our models achieve state-of-the-art accuracy: overall RMSE of 13.40 mm and a correlation coefficient of 0.92 for the regression model, and RMSE of 12.57 mm and a correlation of 0.99 for the Long Short-Term Memory (LSTM) model. These advancements underscore the potential of these models to offer more personalized gait trajectory assistance, improving human–robot interactions.
Anchor Shear Strength Damage under Varying Sand Content, Freeze-Thaw Cycles, and Axial Pressure Conditions
Authors: Dong Jie , Wang Yin-Chen , Wu Zhi-Hui , Gong Feng-Wu , Zhao Ya-Dong , Zhang Hong-Feng
Published: June 2024

Sandy soil in the north of Hebei region of China is widely distributed, the temperature difference between day and night is large, the phenomenon of freezing and thawing is obvious, and the soil body before and after the freezing and thawing cycle of sandy soil slopes is affected by the changes. This paper takes the stability of a sandy soil anchorage interface under a freeze-thaw cycle as the research background and, based on the self-developed anchor-soil interface shear device, analyses the influence of changing sand rate, confining pressure, and the number of freeze-thaw cycles on the shear characteristics of an anchor-soil interface in anchorage specimens. The research findings indicate that, at 50–60% sand contents, the shear strength increases with a higher sand content and is positively correlated with confining pressure within a higher range. A higher sand content stabilises the anchoring body, but an excessively high sand content can lead to failure. Increasing the sand content, confining pressure, and freeze-thaw cycle number all result in a reduction in the shear displacement at the peak strength. After 11 freeze-thaw cycles, the shear strength of the anchoring body stabilises, with a reduction in strength of approximately 32%, and a higher sand content effectively reduces the reduction in strength.
Canonical and Noncanonical Functions of the BH3 Domain Protein Bid in Apoptosis, Oncogenesis, Cancer Therapeutics, and Aging
Authors: Makinwa Yetunde , Luo Yibo , Musich Phillip R. , Zou Yue
Published: June 2024

Effective cancer therapy with limited adverse effects is a major challenge in the medical field. This is especially complicated by the development of acquired chemoresistance. Understanding the mechanisms that underlie these processes remains a major effort in cancer research. In this review, we focus on the dual role that Bid protein plays in apoptotic cell death via the mitochondrial pathway, in oncogenesis and in cancer therapeutics. The BH3 domain in Bid and the anti-apoptotic mitochondrial proteins (Bcl-2, Bcl-XL, mitochondrial ATR) it associates with at the outer mitochondrial membrane provides us with a viable target in cancer therapy. We will discuss the roles of Bid, mitochondrial ATR, and other anti-apoptotic proteins in intrinsic apoptosis, exploring how their interaction sustains cellular viability despite the initiation of upstream death signals. The unexpected upregulation of this Bid protein in cancer cells can also be instrumental in explaining the mechanisms behind acquired chemoresistance. The stable protein associations at the mitochondria between tBid and anti-apoptotic mitochondrial ATR play a crucial role in maintaining the viability of cancer cells, suggesting a novel mechanism to induce cancer cell apoptosis by freeing tBid from the ATR associations at mitochondria.
Preparation, Characterization and Electrochemical Response of Nanostructured TiAlV with Potentiostatically Deposited IrOx as a pH Sensor for Rapid Detection of Inflammation
Authors: Jírů Jitřenka , Hybášek Vojtěch , Michalcová Alena , Korbelová Klára , Koláčný Lukáš , Fojt Jaroslav
Published: June 2024

Electrochemical pH sensors have a wide range of industrial applications such as in medicine due to their fast response and high sensitivity to pH changes. This work focuses on the preparation of samples based on the nanostructure of TiO2 with potentiostatically deposited particles of iridium and its oxides (IrO2), using a Ti-6Al-4V alloy as the base material, and subsequent surface characterization. Transmission electron microscopy and secondary ion mass spectroscopy showed Ir particles distributed in the nanotubes. Using a potentiostatic method, a stable pH sensor was prepared. By monitoring the open circuit potential, it was shown that this sensor is usable even without being kept in a storage medium and does not react to changes in the redox potential of the solution.
Hypovitaminosis D and Leukocytosis to Predict Cardiovascular Abnormalities in Children with Kawasaki Disease: Insights from a Single-Center Retrospective Observational Cohort Study
Authors: Rigante Donato , De Rosa Gabriella , Delogu Angelica Bibiana , Rotunno Giulia , Cianci Rossella , Di Pangrazio Claudia , Sodero Giorgio , Basile Umberto , Candelli Marcello
Published: June 2024

Introduction: An aberrant immune response involving yet unidentified environmental and genetic factors plays a crucial role in triggering Kawasaki disease (KD). Aims: The aim of this study was to assess general and laboratory data at the onset of KD in a single-center cohort of children managed between 2003 and 2023 and retrospectively evaluate any potential relationship with the development of KD-related cardiovascular abnormalities (CVAs). Patients and methods: We took into account a total of 65 consecutive children with KD (42 males, median age: 22 months, age range: 2–88 months) followed at the Department of Life Sciences and Public Health in our University; demographic data, clinical signs, and laboratory variables at disease onset, before IVIG infusion, including C-reactive protein, hemoglobin, white blood cell (WBC) count, neutrophil count, platelet count, aminotransferases, natremia, albumin, total bilirubin, and 25-hydroxyvitamin D were evaluated. Results: Twenty-one children (32.3% of the whole cohort) were found to have echocardiographic evidence of CVAs. Univariate analysis showed that diagnosis of KD at <1 year or >5 years was associated with CVAs (p = 0.001 and p = 0.01, respectively); patients with CVAs had a longer fever duration and mostly presented atypical or incomplete presentations. Interestingly, all patients with CVAs had lower levels of vitamin D (less than 30 mg/dL, p = 0.0001) and both higher WBC and higher neutrophil counts than those without CVAs (p = 0.0001 and p = 0.01, respectively). Moreover, blood levels of albumin were significantly lower in KD patients with CVAs compared to those without (11/21, 52% versus 13/44, 30%, p = 0.02). Multiple logistic regression with correction for sex showed that serum vitamin D < 30 ng/mL, WBC count > 20.000/mm3, and age > 60 months at KD onset were the only independent factors statistically associated with CVAs. Conclusions: Hypovitaminosis D, WBC count over 20.000/mm3, and age above 5 years at KD onset emerged as independent factors statistically associated with the occurrence of CVAs.
Evaluation of Pre-Transfusion Crossmatch Test Using Microscanner C3
Authors: Park Insu , Jang Woong Sik , Lim Chae Seung , Kim Jeeyong
Published: June 2024

A pre-transfusion crossmatch test is crucial for ensuring safe blood transfusions by identifying the compatibility between donor and recipient blood samples. Conventional tube methods for crossmatching have limitations, including subjectivity in result interpretation and the potential for human error. In this study, we evaluated the diagnostic performance of a new crossmatch test using Microscanner C3, which can overcome these shortcomings. The crossmatch test results using the method were obtained in 323 clinical samples. The sensitivity, specificity, positive predictive value, negative predictive value, and concordance rate of the crossmatch test using Microscanner C3 were 98.20%, 100.00%, 100.00%, 98.11%, and 99.07%, respectively. The diagnostic performance of the new system offers a promising alternative to conventional tube methods for pre-transfusion crossmatch testing. Microscanner C3 could also increase the automation, standardization, and accuracy of crossmatch tests. The crossmatch test using Microscanner C3 is thought to increase the efficiency and reliability in identifying blood samples suitable for transfusion, thereby improving patient safety and optimizing the use of blood products in clinical settings.
Efficient Motion Primitives-Based Trajectory Planning for UAVs in the Presence of Obstacles
Authors: Manzoni Marta , Rubinacci Roberto , Invernizzi Davide
Published: June 2024

The achievement of full autonomy in Unmanned Aerial Vehicles (UAVs) is significantly dependent on effective motion planning. Specifically, it is crucial to plan collision-free trajectories for smooth transitions from initial to final configurations. However, finding a solution executable by the actual system adds complexity: the planned motion must be dynamically feasible. This involves meeting rigorous criteria, including vehicle dynamics, input constraints, and state constraints. This work addresses optimal kinodynamic motion planning for UAVs in the presence of obstacles by employing a hybrid technique instead of conventional search-based or direct trajectory optimization approaches. This technique involves precomputing a library of motion primitives by solving several Two-Point-Boundary-Value Problems (TPBVP) offline. This library is then repeatedly used online within a graph-search framework. Moreover, to make the method computationally tractable, continuity between consecutive motion primitives is enforced only on a subset of the state variables. This approach is compared with a state-of-the-art quadrotor-tailored search-based approach, which generates motion primitives online through control input discretization and forward propagation of the dynamic equations of a simplified model. The effectiveness of both methods is assessed through simulations and real-world experiments, demonstrating their ability to generate resolution-complete, resolution-optimal, collision-free, and dynamically feasible trajectories. Finally, a comparative analysis highlights the advantages, disadvantages, and optimal usage scenarios for each approach.
The Opportunity Cost of COVID-19 Deaths in the USA
Authors: Pham Tuyen , Ruhil Anirudh V. S. , Jolley G. Jason
Published: June 2024

The U.S. is currently the country with the highest number of COVID-19 deaths. By the second week of October 2021, over 700,000 people in the U.S. had died after contracting the virus. When estimating the cost and benefit of a COVID-19 prevention measure, the value of a statistical life (VSL) has been widely used as an approximation for the value of a lost life. However, VSL arguably overstates the costs of deaths caused by COVID-19 because VSL captures the private individual’s benefit, and it is the same for everyone regardless of where they live, their productivity, their age, and their gender. In this study, rather than looking at the cost of life loss due to COVID-19, we focus on the opportunity costs of COVID-19 deaths to society. The opportunity cost of COVID-19 deaths is defined as the combination of direct medical costs and the costs of lost potential lifetime earnings. Our analysis focuses on the period from March 2020 to October 2021. We then quantify the average opportunity cost of COVID-19 deaths across the U.S. and by state level.
Physical Activity Enjoyment, Physical Activity Behavior, and Motor Competence in Low-Income Elementary School Students
Authors: Ha Taemin , Fan Xiaoping , Dauenhauer Brian
Published: June 2024

This study aimed to investigate the relationship between low-income elementary school students’ physical activity enjoyment and physical activity behavior and to determine if their motor competence predicts their physical activity enjoyment and physical activity behavior. A total of 82 students (51.2% male; Mage = 10, SD = 0.861) from two Title I elementary schools in the western United States were involved in this study. The students completed the Physical Activity Enjoyment Scale (PACES), the Physical Activity Questionnaire for Children (PAQ-C), and the Test of Gross Motor Development–Third Edition (TGMD-3). The results of the correlation analysis showed that physical activity enjoyment and physical activity behavior among the students were significantly related, r(80) = 0.379, p < 0.001. Multiple regression models indicated that locomotor skills were a significant predictor of the level of physical activity enjoyment (F(2,79) = 8.334, p < 0.001; t = 3.76, p < 0.001), while motor competence did not predict the level of physical activity behavior. These findings illustrate the importance of both physical activity enjoyment and physical activity behavior and the significant role of locomotor skills in enhancing the level of physical activity enjoyment in this specific population of children. Motor competence should be considered a priority when designing and implementing physical education and physical activity programs for school-aged children from lower-income families.
Research on Fixed-Slope On-Chip Soft-Start Method Applied to Buck DC–DC Converter
Authors: Guo Zhongjie , Qiu Ziyi , Yang Yuan , Yu Ningmei
Published: June 2024

A fixed-slope soft-start method applicable to Buck converters for on-chip integration is proposed to address the issue of varying power stresses (device voltage, current stress) during start-up with different output voltages. The main mechanism involves combining feedback coefficient sampling with a fixed-slope reference voltage to achieve a slow rise in the reference voltage by equating the soft-start charging current to a pulse current through the on-chip integration of a small capacitor. This allows for fixed-slope start-ups for different set output voltages. Spike elimination techniques are employed to address charging current spikes caused by pulse periods, enhancing precise control over the soft-start time. By replacing the traditional resistor divider network with a capacitive divider network in the soft-start method, DC power consumption is minimized. Upon completion of the soft start, a smooth transition to a steady-state operation occurs, with the automatic shutdown of the soft-start module reducing static power consumption. A specific circuit design and layout verification based on 0.18 μm high-voltage BCD technology demonstrates that the proposed method maintains a fixed-slope start-up of approximately 5 mV/μs within the chip’s output range of 0.9 V to 4 V, with a slope control accuracy of up to 98%. The soft-start circuit effectively eliminates surge currents generated during start-up under full load conditions of 3 A and no-load conditions of 0 A, reducing the overall surge current by 44% and enabling a stable voltage rise and the smooth transition to a steady-state operation.
The Development of Fast DST-II Algorithms for Short-Length Input Sequences
Authors: Bielak Krystian , Cariow Aleksandr , Raciborski Mateusz
Published: June 2024

The subject of this work is the development of fast algorithms for the discrete sinusoidal transformation of the second type (DST-II) for sequences of input data of small length N = 2, 3, 4, 5, 6, 7, 8. The starting point for the development of algorithms is the well-known possibility of representing any discrete transformation in the form of a matrix–vector product. Due to the remarkable structural properties of the matrices of the DST-II transformation base, these matrices can be successfully factorized, which should lead to a reduction in the computational complexity of the procedure as a whole. You can factorize matrices in different ways. The art of designing fast algorithms is to find the factorization that produces the maximum effect. We justified the correctness of the obtained algorithmic solutions theoretically, using strict mathematical derivations of each of them. The developed algorithms were then further tested using MATLAB R2023b software to finally confirm their performance. Finally, we presented estimates of the computational complexity for each solution obtained and compared them with direct computational methods that rely on the direct calculation of matrix–vector products.
Analysis of Dynamic Parameters of the Switching-On Process of Electromagnetic Relays Powered by Harmonic Polluted Voltage Source
Authors: Smugala Dariusz
Published: June 2024

The article incorporates an analysis of the switching-on process of alternating current (AC), low-voltage (LV), and electromagnetic (EM) relays energized by harmonically polluted voltage sources. In the undertaken research, the dynamic parameters of relays actuated by electromagnetic drive were examined for shape and supply voltage waveform distortion levels as described by different THD factor values. During the research, diverse supplying voltages were delivered to drive electromagnet coils at a variety of phases so as to reflect the stochastic nature of the energizing procedure. The performed analysis allowed for determining the most influential and decisive factors dictating the basic parameter values of the switching-on process. As an outcome, time-related parameters of relay moving armature movement were obtained, and the frequency of disorder occurrences during the switching-on was examined. For experimentation needs, an experiment stand was developed, and dedicated software for measurement results analysis was elaborated. Based on the study results, EM relay switching-on operation reliability was evaluated in view of electric energy quality.
Geochemical Characteristics of Mature to High-Maturity Shale Resources, Occurrence State of Shale Oil, and Sweet Spot Evaluation in the Qingshankou Formation, Gulong Sag, Songliao Basin
Authors: Gao Bo , Feng Zihui , Luo Jinglan , Shao Hongmei , Bai Yubin , Wang Jiping , Zhang Yuxuan , Wang Yongchao , Yan Min
Published: June 2024

The exploration of continental shale oil in China has made a breakthrough in many basins, but the pure shale type has only been found in the Qingshankou Formation, Gulong Sag, Songliao Basin, and the evaluation of shale oil occurrence and sweet spot faces great challenges. Using information about the total organic carbon (TOC), Rock-Eval pyrolysis, vitrinite reflectance (Ro), kerogen elemental composition, carbon isotopes, gas chromatography (GC), bitumen extraction, and component separation, this paper systematically studies the organic geochemical characteristics and shale oil occurrence at the Qingshankou Formation. The G1 well, which was cored through the entire section of the Qingshankou Formation in the Gulong Sag, was the object of this study. On this basis, the favorable sweet spots for shale oil exploration are predicted. It is concluded that the shale of the Qingshankou Formation has high organic heterogeneity in terms of organic matter features. The TOC content of the source rocks in the Qingshankou Formation is enhanced with the increase in the burial depth, and the corresponding organic matter types gradually changed from Ⅱ2 and Ⅱ1 types to the Ⅰ type. The distribution of Ro ranges from 1.09% to 1.67%, and it is the mature to high-mature evolution stage that generates a large amount of normal crude oil and gas condensate. The high-quality source rocks of good to excellent grade are mainly distributed in the Qing 1 member and the lower part of the Qing 2 member. After the recovery of light hydrocarbons and the correction of pyrolytic heavy soluble hydrocarbons, it is concluded that the occurrence state of shale oil in the Qingshankou Formation is mainly the free-state form, with an average value of 6.9 mg/g, and there is four times as much free oil as adsorbed oil. The oil saturation index (OSI), mobile hydrocarbon content, Ro, and TOC were selected to establish the geochemical evaluation criteria for shale oil sweet spots in the Qingshankou Formation. The evaluation results show that interval 3 and interval 5 of the Qingshankou Formation in the G1 well are the most favorable sections for shale oil exploration.
Relationship between Decision-Making Styles and Leadership Styles of Portuguese Fire Officers
Authors: Rouco Carlos , Marques-Quinteiro Pedro , Reis Vítor , Duarte Isabel
Published: June 2024

Numerous studies in management and leadership indicate that one’s decision-making style is reflective of one’s leadership style. In the context of civil protection and the fire service, the ability of the fire officers to adopt the best style of decision-making and leadership in each situation represents a critical factor of success. Therefore, the present study was designed to examine the relationship between decision-making and the leadership style of fire officers. For this study, a quantitative method was used, with the application of questionnaires with closed questions. A sample of 346 officers of Portuguese fire stations answered the questionnaire. Correlations between styles are consistent with findings from other studies. Decision-making styles directly influence the leadership styles adopted. Fire officers prioritize people and social concerns, involving teams in decision-making. Research shows positive correlations between participants’ roles, conceptual decision-making, and relation-focused leadership. Higher academic degrees are associated with relationship-focused leadership. Behavioral decision-making mediates the impact of education on leadership styles.
Contemporary Trends in the Spatial Extent of Common Riverine Fish Species in Australia’s Murray–Darling Basin
Authors: Robinson Wayne , Koehn John , Lintermans Mark
Published: June 2024

As one of the world’s most regulated river basins, the semi-arid Murray–Darling Basin (MDB) in south-eastern Australia is considered at high ecological risk, with substantial declines in native fish populations already identified and climate change threats looming. This places great importance on the collection and use of data to document population trends over large spatial extents, inform management decisions, and provide baselines from which change can be measured. We used two medium-term data sets (10 MDB basin-wide fish surveys from 2004–2022) covering the 23 catchments and 68 sub-catchments of the MDB to investigate trends in the distribution of common riverine species at the entire basin scale. Fifteen native species were analysed for changes in their contemporary range, and whilst short-term changes were identified, all species showed no significant continuous trend over the study period. We further analysed the native species extent relative to their historic records, with bony herring and golden perch occurring in 78% and 68% of their historic river kilometres, respectively, whereas southern pygmy perch, northern river blackfish, silver perch, mountain galaxias, and freshwater catfish were all estimated to occur in less than 10% of their historic extent. Six established non-native species were also analysed and were very consistent in extent over the years, suggesting that they are near the available limits of expansion of their invasion. We provide effect sizes for the spatial extent index which can be used as baselines for future studies, especially those aiming to monitor changes in the spatial extent and population status of native species, or changes in the spatial extent of new or existing non-native species.
Unlocking Phenolic Potential: Determining the Optimal Grain Development Stage in Hull-Less Barley Genotypes with Varying Grain Color
Authors: Friero Iván , Macià Alba , Romero Maria-Paz , Romagosa Ignacio , Martínez-Subirà Mariona , Moralejo Marian
Published: June 2024

Barley is rich in phenolic compounds, providing health benefits and making it a valuable addition to a balanced diet. However, most studies focus on these compounds at barley’s final maturity, neglecting their synthesis during grain development and its impact on barley quality for food applications. This study investigates phenolic profiles during grain development in four hull-less barley genotypes with different grain colors, specifically bred for food applications. The objectives were to determine the phenolic profile and identify the optimal maturity stage for maximum phenolic content and antioxidant capacity. Using UPLC-MS/MS and in vitro antioxidant capacity assays, results show that total phenolic compounds decrease as grain matures due to increased synthesis of reserve components. Flavan-3-ols, phenolic acids, and flavone glycosides peaked at immature stages, while anthocyanins peaked at physiological maturity. The harvest stage had the lowest phenolic content, with a gradient from black to yellow, purple, and blue genotypes. Antioxidant capacity fluctuated during maturation, correlating positively with phenolic compounds, specially bound phenolic acids and anthocyanins. These findings suggest that early harvesting of immature grain can help retain bioactive compounds, promoting the use of immature barley grains in foods. To support this market, incentives should offset costs associated with decreased grain weight.
A Novel Stochastic Model for Human Norovirus Dynamics: Vaccination Impact with Lévy Noise
Authors: Song Yuqin , Liu Peijiang , Din Anwarud
Published: June 2024

The epidemic norovirus causes vomiting and diarrhea and is a highly contagious infection. The disease is affecting human lives in terms of deaths and medical expenses. This study examines the governing dynamics of norovirus by incorporating Lévy noise into a stochastic SIRWF (susceptible, infected, recovered, water contamination, and food contamination) model. The existence of a non-negative solution and its uniqueness are proved after model formulation. Subsequently, the threshold parameter is calculated, and this number is used to explore the conditions under which disease tends to exist in the population. Likewise, additional conditions are derived that ensure the elimination of the disease from the community. It is proved that the norovirus is extinct whenever the threshold parameter is less than one and it persists for Rs>1. The work assumes two working examples to numerically explain the theoretical findings. Simulations of the study are visually presented, and comparisons are made. The results of this study suggest a robust approach for handling complex biological and epidemic phenomena.
Optimizing Economic Dispatch with Renewable Energy and Natural Gas Using Fractional-Order Fish Migration Algorithm
Authors: Aldosary Abdallah
Published: June 2024

This work presents a model for solving the Economic-Environmental Dispatch (EED) challenge, which addresses the integration of thermal, renewable energy schemes, and natural gas (NG) units, that consider both toxin emission and fuel costs as its primary objectives. Three cases are examined using the IEEE 30-bus system, where thermal units (TUs) are replaced with NGs to minimize toxin emissions and fuel costs. The system constraints include equality and inequality conditions. A detailed modeling of NGs is performed, which also incorporates the pressure pipelines and the flow velocity of gas as procedure limitations. To obtain Pareto optimal solutions for fuel costs and emissions, three optimization algorithms, namely Fractional-Order Fish Migration Optimization (FOFMO), Coati Optimization Algorithm (COA), and Non-Dominated Sorting Genetic Algorithm (NSGA-II) are employed. Three cases are investigated to validate the effectiveness of the proposed model when applied to the IEEE 30-bus system with the integration of renewable energy sources (RESs) and natural gas units. The results from Case III, where NGs are installed in place of two thermal units (TUs), demonstrate that the economic dispatching approach presented in this study significantly reduces emission levels to 0.4232 t/h and achieves a lower fuel cost of 796.478 USD/MWh. Furthermore, the findings indicate that FOFMO outperforms COA and NSGA-II in effectively addressing the EED problem.
Short-Term Effects of Two COX-2 Selective Non-Steroidal Anti-Inflammatory Drugs on the Release of Growth Factors and Cytokines from Canine Platelet-Rich Gel Supernatants
Authors: Ospina Julián , Carmona Jorge U. , López Catalina
Published: June 2024

(1) Background: There is a lack of knowledge about how a single dose of COX-2 selective non-steroidal anti-inflammatory drugs (NSAIDs) might affect the release of growth factors (GFs) and cytokines from canine platelet-rich gels (PRGs) and other hemocomponents. (2) Methods: A crossover study was conducted in six adult mongrel dogs. Animals were randomized to receive a single dose of either carprofen or firocoxib. PRG, temperature-induced platelet lysate (TIPL), chemically induced PL (CIPL), and plasma hemocomponents were obtained from each dog before (1 h) and after (6 h) the treatments. Platelet and leukocyte counts and determination of the concentrations of platelet-derived growth factor-BB, (PDGF-BB), transforming growth factor beta-1 (TGF-β1), interleukin 1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α) and IL-10 concentrations were assayed by ELISA in all hemocomponents. (3) Results: Both platelet and leukocyte counts and PDGF-BB concentrations were not affected by NSAIDs and time. Total TGF-β1 concentrations were not affected by NSAIDs; however, the release of this GF was increased in PRG supernatants (PRGS) at 6 h. IL-1β and TNF-α concentrations were significantly (p < 0.001) lower in both firocoxib PRGS and plasma at 6 h, respectively. IL-10 concentrations were significantly (p < 0.001) lower at 6 h in all hemocomponents treated with both NSAIDs. (4) Conclusions: The clinical implications of our findings could indicate that these drugs should be withdrawn from patients to allow their clearance before the clinical use of PRP/PRG. On the other hand, the prophylactic use of NSAIDs to avoid the inflammatory reactions that some patients might have after PRP/PRG treatment should be performed only in those animals with severe reactive inflammation to the treatment.
DNA Testing and Identities in Family History Research
Authors: Shaw Emma L. , Donnelly Debra J. , Boadu Gideon , Burke Rachel , Parkes Robert J.
Published: June 2024

In the preceding decades, rapid technological advancements and increasing democratisation of historical records have been coupled with scientific data from DNA testing, which has revolutionised the family history industry. Going beyond the traditional archives and databases, DNA profiles present nuanced confirmations, puzzles, and contradictions generated through this biological lens. Family history researchers seek iterative engagements with their familial pasts and, in the process, amplify their contemporary identities. This specialised group of historians illuminates their families’ travels through the broader historical landscape, constructing micro-narratives using a broad range of investigative modalities. This article reports on the findings of a large international study (n = 1016) that investigated family history researchers’ motivations for undertaking DNA testing, their experiences, and its impact on their perceptions of individual, national, and global identities using Berzonsky’s socio-cultural model of identity construction (2003, 2011) as an analytic frame. Using a survey methodology, it was concluded that DNA testing can expand and disrupt long-held notions of identity and has the power to shift perceptions and understandings of the self while simultaneously providing a new era of opportunity to reconceptualise national and international affiliations. It suggests further investigative avenues to assess the potential of DNA testing, which may promote social cohesion, inclusiveness, and global citizenship.
Genetic Characterization of 191 Probands with Inherited Retinal Dystrophy by Targeted NGS Analysis
Authors: Mihalich Alessandra , Cammarata Gabriella , Tremolada Gemma , Manfredini Emanuela , Bianchi Marzoli Stefania , Di Blasio Anna Maria
Published: June 2024

Inherited retinal diseases (IRDs) represent a frequent cause of blindness in children and adults. As a consequence of the phenotype and genotype heterogeneity of the disease, it is difficult to have a specific diagnosis without molecular testing. To date, over 340 genes and loci have been associated with IRDs. We present the molecular finding of 191 individuals with IRD, analyzed by targeted next-generation sequencing (NGS). For 67 of them, we performed a family segregation study, considering a total of 126 relatives. A total of 359 variants were identified, 44 of which were novel. Genetic diagnostic yield was 41%. However, after stratifying the patients according to their clinical suspicion, diagnostic yield was higher for well-characterized diseases such as Stargardt disease (STGD), at 65%, and for congenital stationary night blindness 2 (CSNB2), at 64%. Diagnostic yield was higher in the patient group where family segregation analysis was possible (68%) and it was higher in younger (55%) than in older patients (33%). The results of this analysis demonstrated that targeted NGS is an effective method for establishing a molecular genetic diagnosis of IRDs. Furthermore, this study underlines the importance of segregation studies to understand the role of genetic variants with unknow pathogenic role.
An Assessment of the Performance Limitations of the Integrated QuantifilerTM Trio-HRM Assay: A Forensic Tool Designed to Identify Mixtures at the Quantification Stage
Authors: Smith Chastyn , Seashols-Williams Sarah J. , Boone Edward L. , Dawson Green Tracey
Published: June 2024

Although guidelines exist for identifying mixtures, these measures often occur at the end-point of analysis and are protracted. To facilitate early mixture detection, we integrated a high-resolution melt (HRM) mixture screening assay into the qPCR step of the forensic workflow, producing the integrated QuantifilerTM Trio-HRM assay. The assay, when coupled with a prediction tool, allowed for 75.0% accurate identification of the contributor status of a sample (single source vs. mixture). To elucidate the limitations of the developed qPCR-HRM assay, developmental validation studies were conducted assessing the reproducibility and samples with varying DNA ratios, contributors, and quality. From this work, it was determined that the integrated QuantifilerTM Trio-HRM assay is capable of accurately identifying mixtures with up to five contributors and mixtures at ratios up to 1:100. Further, the optimal performance concentration range was found to be between 0.025 and 0.5 ng/µL. With these results, evidentiary-like DNA samples were then analyzed, resulting in 100.0% of the mixture samples being accurately identified; furthermore, every time a sample was predicted as a single source, it was true, giving confidence to any single-source calls. Overall, the integrated QuantifilerTM Trio-HRM assay has exhibited an enhanced ability to discern mixture samples from single-source samples at the qPCR stage under commonly observed conditions regardless of the contributor’s sex.
Optimal Parameters for Gastric Electrical Stimulation Therapy for Long-Term Symptom Control in Patients with Gastroparesis
Authors: Sarosiek Irene , Willauer Alexandra N. , Espino Karina , Sarosiek Jerzy , Galura Gian , Alvarado Luis , Dwivedi Alok , Davis Brian , Bashashati Mohammad , McCallum Richard W.
Published: June 2024

Background: Gastric electrical stimulation (GES) therapy is indicated for the treatment of drug-refractory gastroparesis (GP). However, the long-term effects of GES therapy on GP symptom control and identification of the optimal parameters to activate this long-term efficacy have not been investigated. Methods: We conducted a retrospective cohort analysis of 57 GP patients who received GES and pyloroplasty (PP). The interrogation of the GES system and assessment of GP symptoms were conducted at the initiation of GES therapy and during follow-up visits. We determined the changes in GES parameters including voltage (V), impedance (I), and current (C). The outcome was total symptom score (TSS), which was measured by self-reported GP symptoms. Results: The mean age of patients was 44 (±14) years, and 72% were females. The etiology for GP was diabetes mellitus in 72% and idiopathic in 28%. The median duration of GES follow-up was 47 months (range 5–73) A significant decrease was found in individual symptom scores and the TSS (−10.8; 95%CI: −12.6, −9.08) compared to baseline scores (p < 0.0001). During follow-up, readings for I (515 vs. 598 Ω), V (3.3 vs. 4.8 V), and C (6.5 vs. 8.4 mA) significantly increased (p ≤ 0.0001 for all parameters). Higher GES settings were associated with lower TSS in the adjusted analysis (RC, −1.97; 95%CI: −3.81, −0.12, p = 0.037). Conclusions: these findings suggest that adjusting GES parameters over time based on optimizing symptom improvement should be incorporated into the long-term care of patients receiving gastric neurostimulation therapy.
Disrupted Sensemaking—Understanding Family Experiences of Physical Restraints in ICU: A Phenomenological Approach in the Context of COVID-19
Authors: Flynch Michele , Frederickson Keville
Published: June 2024

Background: The emergence of COVID-19 profoundly influenced the dynamics within intensive care units, significantly altering the patient–family experience. As the pandemic unfolded, the longstanding practice of using physical restraints for patient safety persisted, introducing new challenges in healthcare settings. This study explored the ramifications of these enduring safety measures on family members of ICU patients during the pandemic, illuminating their lived experiences and the psychological impact of seeing their loved ones restrained. Objectives: To explore family members’ lived experiences with physical restraints in the ICU during COVID-19 and inform improvements in patient-centered care. Methods: Utilizing hermeneutic phenomenology, the study engaged ten family members in detailed interviews to gain an understanding of their experiences with ICU physical restraints during COVID-19. Conducted at a northeastern U.S. hospital, the collected narratives underwent thematic analysis within a sensemaking framework, yielding a profound understanding of family perspectives. Results: Family members faced challenges in understanding and coping with physical restraints, revealing a need for improved healthcare system support for family sensemaking and well-being. Conclusions: The study advocates integrating empathetic communication and family engagement into ICU care practices, underlining the importance of sensemaking during healthcare crises.
Polish Cystic Fibrosis Patients’ Health-Related Quality of Life and Its Influencing Factors: A Cross-Sectional, Single-Centre Study
Authors: Humaj-Grysztar Magdalena , Rachel Marta , Bonior Joanna
Published: June 2024

Cystic fibrosis (CF) is a disease characterized by long-term and troublesome symptoms that affect the patient’s life. This study aimed to assess and compare the health-related quality of life (HRQoL) of Polish CF patients and identify factors influencing it. The study group consisted of 79 patients (6 to 42 years old), who filled in an age-appropriate Cystic Fibrosis Questionnaire-Revised. Medical data were collected from each patient’s medical records. The domains with the highest HRQoL median were eating problems (88.89), digestive symptoms (77.78) and physical functioning (75.00). The lowest-rated domain was social functioning (61.90). Age negatively correlated with eight domains, and most strongly with treatment burden (rho = −0.474). Physical functioning positively correlated with all spirometry parameters, and most strongly with FEV1% (rho = 0.588). Treatment burden, body image and respiratory symptoms were positively correlated with all spirometry parameters except PEF%. Present exacerbations reduced scores in almost all domains, and in the MANCOVA model they were a significant factor differentiating patients’ HRQoL. The univariate analysis of MANCOVA showed the significant effects of both health condition (F = 8.32, p = 0.005) and the COVID-19 pandemic (F = 5.89, p = 0.018) on social functioning domain, and of the place of residence on body image (F = 5.60, p = 0.21). A decreasing HRQoL with increasing age and during exacerbations indicates that it is important to focus on these aspects of patients’ lives and ensure they received the necessary support from their healthcare providers.