bluuebunny/crossref_metadata_2025 huggingface dataset:
WEYL REEXAMINED: “DAS KONTINUUM” 100 YEARS LATER
Authors: AVRON ARNON
Published: June 2020

Hermann Weyl was one of the greatest mathematicians of the 20th century, with contributions to many branches of mathematics and physics. In 1918, he wrote a famous book, “Das Kontinuum”, on the foundations of mathematics. In that book, he described mathematical analysis as a ‘house built on sand’, and tried to ‘replace this shifting foundation with pillars of enduring strength’.

In this paper, we reexamine and explain the philosophical and mathematical ideas that underly Weyl’s system in “Das Kontinuum”, and show that they are still useful and relevant. We propose a precise formalization of that system, which is the first to be completely faithful to what is written in the book. Finally, we suggest that a certain set-theoretical modern system reflects better Weyl’s ideas than previous attempts (most notably by Feferman) of achieving this goal.
Pinus cembra L. tree-ring data as a proxy for summer mass-balance variability of the Careser Glacier (Italian Rhaetian Alps)
Authors: Cerrato Riccardo , Salvatore Maria Cristina , Gunnarson Björn E. , Linderholm Hans W. , Carturan Luca , Brunetti Michele , Baroni Carlo
Published: June 2020

Glacial extent and mass balance are sensitive climate proxies providing solid information on past climatic conditions. However, series of annual mass-balance measurements of more than 60 years are scarce. To our knowledge, this is the first time the latewood density data (MXD) of the Swiss stone pine (Pinus cembra L.) have been used to reconstruct the summer mass balance (Bs) of an Alpine glacier. The MXD-based Bs well correlates with a Bs reconstruction based on the May to September temperature. Winter precipitation has been used as an independent proxy to infer the winter mass balance and to obtain an annual mass-balance (Bn) estimate dating back to the glaciological year 1811/12. The reconstructed MXD/precipitation-based Bn well correlates with the data both of the Careser and of other Alpine glaciers measured by the glaciological method. A number of critical issues should be considered in both proxies, including non-linear response of glacial mass balance to temperature, bedrock topography, ice thinning and fragmentation, MXD acquisition and standardization methods, and finally the ‘divergence problem’ responsible for the recently reduced sensitivity of the dendrochronological data. Nevertheless, our results highlight the possibility of performing MXD-based dendroglaciological reconstructions using this stable and reliable proxy.
Obsidian and volcanic glass artifact evidence for long-distance voyaging to the Polynesian Outlier island of Tikopia
Authors: McCoy Mark D. , Cervera Caroline , Mulrooney Mara A. , McAlister Andrew , Kirch Patrick V.
Published: June 2020

Reconstructing routes of ancient long-distance voyaging, long a topic of speculation, has become possible thanks to advances in the geochemical sourcing of archaeological artifacts. Of particular interest are islands classified as Polynesian Outliers, where people speak Polynesian languages and have distinctly Polynesian cultural traits, but are located within the Melanesian or Micronesian cultural areas. While the classification of these groups as Polynesian is not in dispute, the material evidence for the movement between Polynesia and the Polynesian Outliers is exceedingly rare, unconfirmed, and in most cases, nonexistent. We report on the first comprehensive sourcing (using a portable X-ray fluorescence spectrometer) of obsidian and volcanic glass artifacts recovered from excavations on the Polynesian Outlier island of Tikopia. We find evidence for: (1) initial settlement followed by continued voyages between Tikopia and an island Melanesian homeland; (2) long-distance voyaging becoming much less frequent and continuing to decline; and (3) later voyaging from Polynesia marked by imports of volcanic glass from Tonga beginning at 765 cal yr BP (±54 yr). Later long-distance voyages from Polynesia were surprisingly rare, given the strong cultural and linguistic influences of Polynesia, and we suggest, may indicate that Tikopia was targeted by Tongans for political expansion.
STABLE SOLUTIONS TO THE STATIC CHOQUARD EQUATION
Authors: LE PHUONG
Published: June 2020

This paper is concerned with the static Choquard equation $$\begin{eqnarray}-\unicode[STIX]{x1D6E5}u=\bigg(\frac{1}{|x|^{N-\unicode[STIX]{x1D6FC}}}\ast |u|^{p}\bigg)|u|^{p-2}u\quad \text{in }\mathbb{R}^{N},\end{eqnarray}$$ where $N,p>2$ and $\max \{0,N-4\}<\unicode[STIX]{x1D6FC}<N$. We prove that if $u\in C^{1}(\mathbb{R}^{N})$ is a stable weak solution of the equation, then $u\equiv 0$. This phenomenon is quite different from that of the local Lane–Emden equation, where such a result only holds for low exponents in high dimensions. Our result is the first Liouville theorem for Choquard-type equations with supercritical exponents and $\unicode[STIX]{x1D6FC}\neq 2$.
Transcriptional regulation mechanism of sterol regulatory element binding proteins on Δ6 fatty acyl desaturase in razor clamSinonovacula constricta
Authors: Ran Zhaoshou , Kong Fei , Xu Jilin , Liao Kai , Yan Xiaojun
Published: June 2020

The razor clam,Sinonovacula constricta, contains high levels of long-chain PUFA (LC-PUFA), which are critical for human health. In addition,S. constrictais the first marine mollusc demonstrated to possess Δ6 fatty acyl desaturase (Fad) and complete LC-PUFA biosynthetic ability, providing a good representative to investigate the molecular mechanism of sterol regulatory element binding proteins (SREBP) in regulating Δ6 Fad for LC-PUFA biosynthesis in marine molluscs. Herein,S. constrictaSREBP and Δ6 Fad promoter were cloned and characterised. Subsequently, dual luciferase and electrophoretic mobility shift assays were conducted to explore the SREBP binding elements in the core regulatory region ofS. constrictaΔ6 Fad promoter. Results showed thatS. constrictaSREBP had a very conservative basic helix-loop-helix-leucine zipper motif, whileS. constrictaΔ6 Fad promoter exhibited very poor identity with teleost Fads2 promoters, indicating their differentiation during evolution. A 454 bp region harbouring a core sequence inS. constrictaΔ6 Fad promoter was predicted to be essential for the transcriptional activation by SREBP. This was the first report on the regulatory mechanism of LC-PUFA biosynthesis in marine molluscs, which would facilitate optimising the LC-PUFA biosynthetic pathway of bivalves in further studies.
Relationship of household cooking salt and eating out on iodine status of pregnant women in environmental iodine-deficient coastal areas of China
Authors: Wang Zhengyuan , Jin Wei , Zhu Zhenni , Cui Xueying , Song Qi , Shi Zehuan , Wu Chunfeng , Zang Jiajie , Guo Changyi
Published: June 2020

As city residents eat out more frequently, it is unknown that if iodised salt is still required in home cooking. We analysed the relationship of household salt and eating out on urinary iodine concentration (UIC) in pregnant women. A household condiment weighing method was implemented to collect salt data for a week. A household salt sample was collected. A urine sample was taken at the end of the week. Totally, 4640 participants were investigated. The median UIC was 139·1 μg/l in pregnant women and 148·7, 140·0 and 122·9 μg/l in the first, second and third trimesters. Median UIC in the third trimester was lower than in the other trimesters (P < 0·001). The usage rates of iodised (an iodine content ≥ 5·0 mg/kg) and qualified-iodised (an iodine content ≥ 21·0 mg/kg) salt were 73·9 and 59·3 %. The median UIC in the qualified-iodised salt group was higher than in the non-iodised group (P = 0·037). The median UIC in the non-iodised group who did not eat out was lower than in qualified-salt groups who both did and did not eat out (P = 0·007, <0·001). The proportion of qualified-iodised salt used in home cooking is low, but foods eaten out have universal salt iodisation according to the national compulsory policy. Household iodised salt did not play a decisive role in the iodine status of pregnant women. Pregnant women in their third trimester who are not eating out and using non-iodised salt at home require extra iodine.
A NEW PORPHYRY FRAGMENT?
Authors: Freudenthal Gad , Johnson Aaron P.
Published: June 2020

The days, not so far back, in which Arabic philosophical works were skimmed essentially with a view of ‘uncovering’ lost gems of Greek philosophy are fortunately behind us. Today these works are studied on their own, as essential building blocks of the history of philosophy. None the less, medieval philosophic works in Arabic continue to allow significant new discoveries concerning the history of Greek philosophy. The same holds, naturally enough, of medieval Hebrew works written by Jewish scholars who lived under the Crescent and accessed Arabic sources.
Tracing multiple sources of sediments using trace element and Nd isotope geochemistry: provenance of the Mesozoic succession in the Kutch Basin, western India
Authors: Chaudhuri Angana , Chatterjee Anirban , Banerjee Santanu , Ray J.S.
Published: June 2020

An integrated approach involving Sr–Nd isotope, trace and rare earth element analyses tracks multiple sources of the Mesozoic sediments of the Kutch Basin at the western continental margin of India. High (87Sr/86Sr)t (ratio at time of deposition), negative εNd and high concentrations of large-ion lithophile elements (LILEs) indicate the upper continental source. Ratios of Nb/Ta and Zr/Hf suggest sedimentary and felsic igneous sources of sediments. The moderate to high concentration of La, Th and Sc, light rare earth elements (LREE-) enrichment, weak negative Eu anomalies and the relationship between εNd(0) and Th/Sc indicate the dominantly felsic composition of source rocks. However, low contents of Th, low values of (87Sr/86Sr)t and depleted mantle model age TDM < 1600 Ma indicate input from a younger mafic source. Increasing concentrations of Zr, Hf and Nd isotopes and a gradual increase in mean TDM from the older to the younger formations indicate erosional unroofing at the source terrain. The increasing (87Sr/86Sr)t through time relates to increased weathering of the source rock. The overwhelmingly southwesterly palaeocurrent direction of current-generated sedimentary structures, and the mean TDM ages trace suggest source areas of the Kutch Basin to Precambrian rocks in the north and NE of this basin. The TDM ages highlight the dominance of late Palaeoproterozoic source rocks. Nd isotope composition indicates that Proterozoic rocks of Marwar Supergroup and Erinpura Granite, in particular, served as main sediment contributors for the Mesozoic sediments in Kutch. We therefore conclude that the Mesozoic sediments in the Kutch Basin are predominantly of late Palaeoproterozoic age with lesser inputs from rocks of early Mesoproterozoic and early Palaeoproterozoic age.
Angiogenesis in cardiopulmonary dirofilariosis: does the Wolbachia surface protein have a pro- or anti-angiogenic effect?
Authors: Zueva T. , Morchón R. , Carretón E. , Ollauri-Ibáñez C. , Pericacho M. , Rodríguez-Barbero A. , Simón F.
Published: June 2020

Cardiopulmonary dirofilariosis caused by Dirofilaria immitis produces inflammation, blood vessel obstruction and hypoxia, which are required conditions for the beginning of the process of neovascularization. Since D. immitis harbours intracellular symbiotic Wolbachia bacterium, the global understanding of the angiogenic process requires the analysis of the effect of the parasite molecules, but also that of Wolbachia. Canine primary lung microvascular endothelial cells were treated with the recombinant Wolbachia surface protein (rWSP) and the expression of angiogenic factors like Vascular Endothelial Growth Factor-A (VEGF-A), sFlt, membrane Endoglin (mEndoglin) and soluble Endoglin (sEndoglin), as well as the in vitro formation of pseudocapillaries, were measured. The analyses showed a significant increase in the expression of pro-angiogenic VEGF-A and anti-angiogenic sEndoglin, together with a significant decrease in both pro-angiogenic mEndoglin and pseudocapillary formation, compared to untreated controls. Due to the complexity of the angiogenic process and its relationship with other physiological processes like inflammation and fibrinolysis, these results might suggest that rWSP participate in various mechanisms related to each other and its effects might depend either on the balance between them or on the moment of their occurrence.
Intakes and status of riboflavin in a representative sample of Irish adults aged 18–90 years screened for MTHFR C677T polymorphism
Authors: O'Sullivan Emma , Kehoe Laura , Walton Janette , McNulty Helene , Ward Mary , McAnena Liadhan , Hopkins Sinead M. , McNulty Breige A. , Nugent Anne P. , Flynn Albert
Published: June 2020

Meta-analyses of epidemiological data report that adults who carry a common polymorphism, the MTHFR 677C→T, in the gene encoding the folate-metabolising enzyme methylenetetrahydrofolate reductase (MTHFR) have a 40% increased risk of CVD and an 87% increased risk of hypertension. Riboflavin (vitamin B2), in its co-enzymatic form flavin adenine nucleotide (FAD), is required as a co-factor by MTHFR and previous trials in hypertensive patients have shown a blood pressure lowering response to riboflavin supplementation that is specific to individuals homozygous for this polymorphism (TT genotype). Low folate status is commonly reported in adults with the TT genotype however the effect of this genetic variant on riboflavin status has not previously been investigated. The aim of this study, therefore, was to investigate dietary intake and biomarker status of riboflavin by MTHFR genotype in Irish adults using data from the National Adult Nutrition Survey (2008–2010) (www.iuna.net).

A 4-day semi-weighed food record was used to collect food and beverage intake data from a representative sample of 1500 Irish adults (18–90 years). Dietary intake data were analysed using WISP© based on UK food composition tables (modified to include recipes of composite dishes, nutritional supplements, fortified foods and generic Irish foods that were commonly consumed). Usual intakes were calculated via the NCI-method using SAS© Enterprise Guide. Blood samples (n = 1126) were collected by venepuncture by a trained professional and were processed and analysed using standard operating procedures. Biomarker status of riboflavin was determined by erthyrocyte gluthathione reductase activation coefficient (EGRac), a functional assay that measures the activity of the enzyme glutathione reductase before and after in vitro activation with its prosthetic group FAD; a lower value indicates better status.

It was found that 12% of the population had the TT genotype. As expected, there was no significant difference in riboflavin intake across the genotype (CC, CT or TT) groups. Similarly, no significant genotype differences in riboflavin status (EGRac) were observed (1.36 vs 1.37 vs 1.38 respectively). Overall, 61% of the total population had EGRac values > 1.3, indicative of low/deficient status with no significant difference observed between the genotype groups (60%,61% and 61%, respectively).

These data suggest that riboflavin status is not influenced by the C677T polymorphism in MTHFR in this cohort of nationally representative Irish adults. Further research is needed to see the impact of riboflavin status on blood pressure across the genotype groups in this nationally representative cohort of Irish adults.
Get A Move On: Using intelligent personal systems to promote behaviour change within the home setting – A process evaluation
Authors: Logue Caomhan , Flynn Jonathan , Gallagher Alison , Murphy Marie , Carlin Angela
Published: June 2020

Approximately one quarter of children living in Northern Ireland are overweight or obese. Intelligent personal systems (IPS) such as Amazon Echo and Google Home have become increasingly integrated into the home setting and therefore, may facilitate behaviour change via novel interactions or as an adjunct to conventional interventions. However, little is currently known about their potential role in this context; therefore, the aim of this feasibility study is to assess the effect of a home-based technology intervention (delivered using Amazon Echo) on physical activity (PA) and dietary habits in families attending the Safe Wellbeing Eating & Exercise Together (SWEET) project, a community-based health promotion programme. Recruitment to the study is ongoing with the aim of recruiting up to 16 families. Families are randomly assigned to receive an IPS (n = 8) or assigned to control (n = 8) i.e. attend the SWEET project as usual, for 12 weeks. Individualised prompts and reminders, aligned with the content of the SWEET project, are regularly delivered to families via the IPS and normal interaction with the device is also encouraged. The primary outcome measure is PA, which will be objectively measured using an Actigraph accelerometer, and secondary outcome measures include body mass index (BMI) and family eating and activity habits. Process evaluation data from focus groups and device interaction will be used to determine the feasibility of using IPS to promote healthy behaviours within the home setting. To date, 11 families have been recruited (11 adults, 90.9% F; 16 children, 56.3% F), mean age 40.4 ± 5.5 years and BMI 34.9 ± 6.7 kg/m2 for adults and 8.9 ± 2.1 years and BMI z-score 2.61 ± 1.23 for children. Average moderate-to-vigorous intensity physical activity (MVPA) was dichotomised to determine the percentage of adults and children meeting the UK (2011) PA guidelines for health. In total, 62.5% of adults reached the recommended level of 150 minutes MVPA per week before the intervention (n = 8;191.50 ± 81.10 minutes), with 40% of children reaching the recommended level of 60 minutes MVPA each day of the week (n = 10; M = 52.83 ± 31.07 minutes). Follow-up measurements will be taken at the end of the intervention and acceptability and usability of such devices within the context of promoting healthy behaviours will be assessed. The findings from this feasibility study will demonstrate whether the use of IPS can increase PA in adults and children, as well as provide novel insights into the feasibility of using these devices to facilitate behaviour change.
A digital meal nutritional score may influence food choices in a workplace restaurant: A pilot study
Authors: Prozorovscaia Daniela , Jacquier Emma , Dudan Florent , Mungkala Suttipong , Green Hilary
Published: June 2020

Introduction

Food choice is complex. Digital nutrition applications are emerging to help decisions about food choices. Nestlé's Meal Nutritional Score (MNS) is a number between 0 and 100 that provides a measure of the extent to which a meal meets US dietary recommendations. The objective was to evaluate if the MNS influences food choices in a workplace restaurant.

Materials and Methods

A workplace education campaign ran over two weeks in order to introduce the MNS to employees. This was done during the lunch break at the entrance to the cafeteria of Nestlé's research centre, which serves around 250 hot meals at lunchtime every day. Employees choose between a typical Western meal, a healthy meal and a vegetarian meal. During the campaign, and for three weeks afterwards, LED screens displayed the MNS for the three different types of meal, every day. Employees’ voluntary feedback on the MNS was collected using a closed-ended questionnaire. Descriptive analyses were done for the scores and sales of each type of meals for one week before the education campaign, immediately after the campaign and three weeks later. Data are reported as mean ± 1 standard deviation.

Results

Feedback was obtained from 152 employees, of whom 96% said the MNS helped them to understand the nutritional balance of the meals, and 38% said the MNS influenced their meal choices. The MNS scores pre-campaign, post-post campaign and 3 weeks later were 52 ± 14, 50 ± 16 and 56 ± 11 for the Western meal; 54 ± 14, 62 ± 6 and 67 ± 6 for the healthy meal and 64 ± 11, 57 ± 14 and 57 ± 12 for the vegetarian meal, respectively. The percentage of sales pre-campaign, post-post campaign and 3 weeks later were 48 ± 10%, 43 ± 9% and 33 ± 9% for the Western meal; 25 ± 10%, 30 ± 8% and 36 ± 10% for the healthy meal, and 27 ± 4%, 27 ± 4% and 30 ± 8% for the vegetarian meal, respectively.

Discussion

The number of people selecting the healthy meal, which usually had the best score, increased during the three weeks following the education campaign, suggesting that the MNS positively influences food choices. The MNS may also help chefs to design more nutritionally balanced meals. Longer-term follow up is necessary to evaluate if these are sustained behaviour changes as well as to test the impact of the MNS in a different workplace environment.
Glycine contributes to the control of hepatic response to insulin by promoting mitochondrial-endoplasmic reticulum interactions
Authors: Alves Anaïs , Bulteau Anne-Laure , Pirola Luciano , Rieusset Jennifer , Morio Béatrice
Published: June 2020

Introduction

Determinants of health and diseases in humans involve complex interactions between diet, gut microbiota and host metabolism. The liver is a major organ that coordinates host adaptations to environmental factors. Mitochondria and endoplasmic reticulum tightly regulate liver nutrient sensing and metabolic adaptations, shaping its metabolic flexibility. Both organelles interact through contact points (named MAMs) in order to exchange calcium and lipids, and MAMs’ integrity settles metabolic flexibility of the liver. Disrupted MAMs’ integrity in obesity is linked to liver steatosis and insulin resistance. This occurs in association with alterations circulating amino acid profiles, among which glycine. Meta-analyses showed that the low plasma glycine concentration observed in obesity is a predictive factor for developing type 2 diabetes. We thus aimed at exploring whether glycine could participate to the regulation of the hepatic response to insulin and whether mechanisms may involve regulation of mitochondrial-ER (MAMs) interactions.

Materials and Methods

The study was carried out in 12 week-old male C57B16J mice fed a standard chow (n = 12/group, 36 total) in accordance with the French guidelines for the care and use of animals. Glycine (1.2g/kg) was provided for 3 days in drinking water vs. water vs. isonitrogenous placebo amino acids. Liver was collected after an overnight fast, 15 min after ip injection of saline (n = 6/group) or insulin (0.75U/kg, n = 6/group). Liver samples were fixed in glutaraldehyde/cacodylate for quantifying MAMs by transmission electronic microscopy. Fractions enriched in MAMs were isolated by differential ultracentrifugation on fresh tissue for Western Blot explorations. Insulin response was assessed through Akt phosphorylation at Ser473.

Results

Glycine supplementation increased the percentage of interaction between ER and mitochondria (compare to mitochondrial surface) for all spacing from 10 to 50 nm. Thus, glycine supplementation induced a 45% increase in mitochondrial-ER (MAMs) interactions compared to other groups (p < 0.001). In agreement to the improved MAMs’ integrity, insulin response was enhanced by 50% after glycine supplementation compared to other groups (p < 0.05). Finally, protein analysis of the MAMs' fraction corroborates the importance of PP2A as a key enzyme regulating MAMs’ integrity.

Conclusion

Glycine is therefore an interesting nutritional actor that can play a crucial role in regulating the integrity of MAMs in the liver, thus contributing to the control of the insulin response.
Vitamin D bioavailability from different lipid delivery systems
Authors: McCourt Aislinn , O'Sullivan Aifric
Published: June 2020

Introduction

Data modelling studies confirm a role for food fortification to improve vitamin D intakes and status. However, there is very little research reporting the bioavailability of vitamin D from foods to inform food fortification strategies. There is some evidence to suggest that the lipid component of foods impacts vitamin D uptake in cells; however, no studies have examined the absorption kinetics of vitamin D from different lipid delivery systems in humans. The aim of this research is to compare changes in serum vitamin D concentrations using different vitamin D emulsions as delivery systems.

Methods

A single blind, controlled, 4-way crossover postprandial study was conducted. Participants consumed 4 different 20μg vitamin D enriched emulsion drinks (olive oil emulsion, fish oil emulsion, mixed micelle emulsion and a non-fat control drink) with a low-fat breakfast on 4 occasions separated by a 1-week washout. Serum 25-hydroxyvitamin D (25(OH)D3) concentrations and cardiometabolic risk markers were measured at baseline and 2, 4, 6 and 8 hours after consuming the drink. Statistical analysis was performed in IBM SPSS Statistics, Version 24 (SPSS Inc. Chicago, IL, USA). Repeated measures ANOVA was performed to assess the effects of the 4 treatments.

Results

Six males and six females aged between 22 and 65 years completed the study. All participants were healthy with a mean BMI of 24.7 ± 4.1kg/m2 for males and 25.1 ± 4.5kg/m2 for females. Each 100 ml drink contained 106 kcal, 4 g fat, 11.9 g carbohydrate and 5.5 g protein, apart from the control which contained 70kcal and 0 g fat. The breakfast contained 321 kcal, 0.7 g fat, 66 g carbohydrate and 10.3 g protein. There was an increase in 25(OH)D3 concentrations over the 8-hour assessment period following consumption of all 4 drinks. Two-way repeated measures ANOVA showed a significant drink*time interaction. No participants experienced any negative side effects after consuming any of the drinks.

Discussion

The absorption kinetics of vitamin D is different depending on the lipid delivery system. This is the first human study demonstrating the bioavailability of vitamin D3 from various emulsions including a novel mixed micelle emulsion. All emulsions were palatable, effective and safe to consume.
Breakfast skipping and risk of cardiovascular disease: A systematic review and meta-analysis of prospective studies
Authors: Morze Jakub , Danielewicz Anna , Rynkiewicz Andrzej , Przybylowicz Katarzyna
Published: June 2020

Breakfast is considered to be the most important meal of the day, which significantly contributes to overall dietary quality. Global trends suggest a decline in breakfast consumption frequency in all age groups over the years. Breakfast skipping is associated with increased risk of weight gain, insulin resistance and type 2 diabetes, which are crucial risk factors of cardiovascular diseases (CVD). Regular breakfast consumption as a modifiable risk factor might be of great interest for cardiovascular prevention. Several prospective cohort studies showed contradictory findings on breakfast skipping and CVD risk. To the best knowledge of authors, this topic was not covered by any previous meta-analysis. Therefore, this review aimed to summarise the evidence on the association between breakfast skipping and CVD risk in prospective studies with particular emphasis on dose-response relationship and quality of existing evidence. A comprehensive search of the literature was performed using PubMed and Embase databases until February 2019, with no restriction to language and date. Inclusion criteria were as following: (1) participants aged ≥ 18 years, (2) prospective design, (3) information about breakfast consumption/skipping frequency and (4) outcome is the risk of CVD including coronary heart disease, stroke or heart failure. For high versus low comparison, random effects model was applied using the inverse variance method. Greenland-Longenecker method was used to conduct linear dose-response meta-analysis. Potential nonlinear association were examined using restricted cubic splines model. Heterogeneity was evaluated using the Cochrane Q test and I2 statistic with value > 50% indicating substantial heterogeneity. Quality of evidence was assessed using NutriGrade tool. Out of 877 records identified in the literature search, four prospective cohort studies with 7258 CVD cases were included. Comparing breakfast skipping to regular breakfast consumption, there was a positive association with CVD risk (RR = 1.17 95%CI: 1.02–1.34, I2 = 39%, pheterogenity = 0.14). In linear dose-response meta-analysis, each additional day of breakfast skipping was related to 2% higher risk of CVD (RR = 1.02 95%CI: 1.00–1.04, I2 = 15%, pheterogenity = 0.31, n = 3). No non-linear association was observed (pnon-linearity = 0.53, n = 3). The quality of evidence for these associations was rated as low. This review indicated that breakfast skipping is associated with increased risk of CVD. Potential implementation of these findings in cardiovascular prevention has to consider low trust in existing evidence. Further prospective studies on this topic have to focus on the type of foods and meals consumed during breakfast, as well as BMI mediation.
Impact of daily protein and energy intake and distribution on muscle mass and strength in Danish older individuals-The CALM study
Authors: Nishimura Yusuke , Hojfeldt Grith , Schacht Simon Rønnow , Mertz Kenneth , Hjulmand Morten , Reitelseder Søren , Lind Mads Vendelbo , Tetens Inge , Holm Lars
Published: June 2020

The importance of dietary protein for the maintenance of muscle mass and strength is heavily discussed. However, adequate energy intake is an underlying assumption but often not considered. In this study, we investigated the impact of daily intake and meal distribution of both protein and energy on muscle mass and strength. In a cross-sectional study, a total of 184 older individuals (gender: 86F/98M, age: 70.2 ± 3.9 yrs, BMI: 25.4 ± 3.7 kg/m2; means ± SD) were recruited. Participants underwent a 3-day weighed dietary record, Dual-energy X-ray Absorptiometry (DXA) scan, hand-grip strength, and Maximal Voluntary Isometric knee-extension Contraction (MVIC). Participants were divided into two categories: lower (LOW; < 0.83 g/adjusted(a)BW/day) or higher (HIGH; ≥ 1.1 g/aBW/day) protein intake levels analysed by gender to characterize a daily protein and energy intake pattern. Main meal protein and energy intake distributions were calculated, and correlations were made. Further, energy intake at breakfast and lunch divided by total energy intake (energy ratio) was correlated with appendicular skeletal muscle index (ASMI), hand-grip strength, and MVIC were determined using the LOW/HIGH-protein-intake categorization. Further, gender-specific ASMI, hand-grip strength and knee extension were compared based on the following four distinct daily protein intake ranges: < 0.66; ≥ 0.66- < 0.83; ≥ 0.83- < 1.1; ≥ 1.1 g/aBW/day. A positive correlation appeared between protein and energy intake in all main meals (r ≥ 0.57, p < 0.0001). In the LOW category, positive correlations were found between energy ratio and ASMI (r = 0.16, p = 0.048), hand-grip strength (r = 0.40, p = 0.0009), and MVIC (r = 0.36, p = 0.0019), whereas no associations were found in the HIGH category. ASMI, hand grip, and MVIC were similar regardless of the protein intake ranges, though with women being lower than men (p < 0.05) in all outcomes. These results show that total daily protein intake did not affect muscle mass and strength in our cohort. However, our data demonstrate that greater energy intake in breakfast and lunch relative to total energy intake is associated with higher muscle mass and strength, particularly when protein intake is lower than 0.83 g/aBW/day, indicating the potential importance of meal energy content at lower intakes of protein.
Nutrient-dense, pesco-vegetarian, affordable, low-calorie, and quick recipes support a reduction in climate impact of diets
Authors: van Dooren Corné
Published: June 2020

Introduction

Educating cooking skills through applying healthy recipes is an effective intervention to stimulate healthy diets. The Netherlands Nutrition Centre applies this strategy by distributing recipes via websites, recipe books, and newsletters. The recipe database exists of 1891 recipes of which 1188 are main courses. In 2018, the recipes website was consulted through 338.598 unique pageviews. Healthier diets tend to be also more sustainable. This study analyses the climate impact of the recipes and its relationship with recipe characteristics.

materials and methods

1188 main courses were analysed on their popularity (top 100 > 500 pageviews/y), climate impact, vegetarian or not, preparation time (< 15 minutes, 15–30 min, > 30 min), price (‘budget’ < €2.25 pp), kilocalorie content (low < 525kcal) and nutrient density. The environmental impact was calculated by summing the greenhouse gas emissions (GHGE) of the ingredients (CO2eq-ingr) and of the energy used for preparation (CO2eq-prep), together CO2eq-tot. Recipes with GHGE lower than 1300 g CO2eq-tot were labelled as ‘Klimaatkanjer’ (climate topper). Nutrient density was estimated with the Sustainable Nutrient-Rich Foods index (SNRF). Differences between recipe groups (t-test and Chi-square) and regressions (ANOVA) between characteristics were statistically analysed.

results

The average GHGE of the recipes is per person 1365 g CO2eq-ingr plus 200 g CO2eq-prep (1566 g CO2eq-tot). Popular recipes do not differ significantly from average (1680g). ‘Klimaatkanjer’ (754 versus 2277g), vegetarian (812 vs 2132g), nutrient-dense (SNRF > 1; 1087 vs 1880g), fish (1483 vs 2604 g meat), budget (1312 vs 1695g), and low-calorie recipes (1486 vs 1653g) have significant lower GHGE. The longer the preparation time, the higher GHGE. Popular recipes are more often budget (p = 0.01) and less often low-calorie (p = 0.03), but they do not significantly differ in vegetarian (p = 0.14), ‘Klimaatkanjer’ (p = 0.15), nutrient density (p = 0.09), or preparation time (p = 0.06). Recipes show a positive correlation between GHGE and kcal (R = 0.313, p < 0.01), GHGE and protein content (R = 0.508,p < 0.01), GHGE and saturated fat content (R = 0.399, p < 0.01), and a negative between GHGE and SNRF (R = 0.188,p < 0.01). 90% of the healthy recipes has a positive SNRF, of which 36% SNRF > 1.

discussion

Healthy recipes are supposed to have a lower climate impact than unhealthy ones. But the climate impact of diets can be further reduced by choosing and promoting recipes that are highly nutrient-dense (SNRF > 1), (pesco-)vegetarian, affordable (< €2.25), low-calorie (< 525kcal) and/or quick to prepare (< 15min). Promoting affordable recipes as intervention is most promising because they are already more popular among Dutch consumers.
Appetite and physiological responses to medium-chain triglycerides (MCT) and conjugated linoleic acid (CLA) in lean vs overweight/obese participants
Authors: Maher Tyler , Deleuse Martina , Thondre Sangeetha , Clegg Miriam
Published: June 2020

Medium-chain triglycerides (MCT) are lipids which have been shown to yield appetite-suppressing properties compared to long-chain triglycerides (LCT), which are more commonly found in the diet. Conjugated linoleic acid (CLA) has also been shown to increase satiety and decrease subsequent energy intake, although CLA has received much less attention to date. The mechanisms behind the appetite-suppressing properties are yet to be fully elucidated. Furthermore, no study has directly compared these effects or mechanisms in overweight/obese individuals to a group of healthy-weight weight controls.

Fifteen healthy-weight (BMI: 22.7 ± 1.9 kg/m2) and fourteen overweight/obese (BMI: 30.9 ± 3.9 kg/m2) individuals participated in a randomised single-blind cross-over study. Overnight fasted participants were fed smoothie breakfasts containing either 23.06 g vegetable oil (LCT), 25.0 g MCT oil (MCT), or 6.25 g CLA and 16.8 g vegetable oil (CLA) on separate test days. Subjective appetite ratings, peptide YY (PYY), total ghrelin, β-hydroxybutyrate, and gastric emptying (GE) were measured throughout each trial. Energy intake in the laboratory was measured at a buffet meal 3-h after the smoothie breakfast and for the following ~36-h in their free-living environment via food records.

Energy intake and subjective appetite ratings were not affected by weight status, so results are presented as a whole. MCT reduced energy intake compared to LCT over the trial day and whole 48-h period, but not at the ad libitum lunch. There was no difference between CLA and LCT at any time point. There were no differences in postprandial subjective appetite ratings between trials. PYY concentrations were not affected by weight status (P = 0.170), and did not differ between trials (P = 0.743) and total ghrelin was also not affected by weight status (P = 0.306), and concentrations were similar between trials (P = 0.188). β-hydroxybutyrate concentrations were higher in the overweight/obese group compared to the lean group (P = 0.009), and MCT led to increased concentrations compared to both LCT (P = 0.005) and CLA (P < 0.001). MCT delayed GE half-time (P < 0.001), lag-phase (P < 0.001), latency phase (P = 0.01), and ascension time (P < 0.001) compared to LCT, with no differences in GE between LCT and CLA (all P > 0.05).

MCT consumption reduces intake over the subsequent 48-h, whereas CLA has no effect compared to LCT. This does not appear to be related to PYY or total ghrelin, but may be mediated by β-hydroxybutyrate or via a delay of gastric emptying.
Iodine status in breastfeeding women and their infants in New Zealand
Authors: Jin Ying , Coad Jane , Brough Louise
Published: June 2020

Iodine is an essential micronutrient in thyroid hormone synthesis and physical and mental development. Iodine deficiency has historically been a problem in New Zealand. Two government initiatives were introduced: 1) mandatory fortification of all bread (except organic) with iodised salt (2009); 2) provision of a subsidised iodine supplement for all pregnant and breastfeeding women (2010). Since these initiatives, the majority of adults and children have achieved adequate iodine status. However, iodine status among breastfeeding women and their infants is unknown. This observational follow-up cohort study recruited 87 breastfeeding women, aged 16 years and over, with a healthy term singleton infant, in the Manawatu region, New Zealand. Women were recruited at 3 months postpartum; and followed up at 6 and 12 months postpartum. A maternal questionnaire investigated supplement use before and after delivery, iodine nutritional knowledge, and demographic information. Maternal spot urine, breastmilk, and infant spot urine samples were collected and iodine concentrations determined using inductively-coupled plasma mass spectrometry. Iodine-containing supplement use was recorded in the 24 hours before biological sample collection. Shapiro-Wilk test was used to check the normality of the data. Iodine data were analysed using non-parametric Mann Whitney U test (2-tailed).Thirty-five (40%) women used iodine-containing supplements during breastfeeding. Median urine iodine concentration (UIC) was 82 (46, 157) μg/L, below the WHO cut-off value of 100 μg/L for breastfeeding women. Median infant UIC was 115 (69, 182) μg/L, above the WHO cut-off value of 100 μg/L. Median maternal UIC was significantly higher for supplement users than non-users (111(44, 240) vs 62(42, 102) μg/L; p = 0.021]. Median infant UIC was significantly higher for supplement users than non-users (150(97, 193) vs 86(53, 171) μg/L; p = 0.045]. Median breastmilk iodine concentration (BMIC) was 69 (52, 119) μg/L, lower than recommended (75 μg/L). Median BMIC was significantly higher for supplement users than non-users [83(67, 168) vs 62(48, 82) μg/L; p = 0.000]. Overall, iodine status within this sample of breastfeeding women remains inadequate at 3 months postpartum. Users of iodine containing supplements and their infants achieved recommended iodine UIC concentrations; however, non-users and their infants had inadequate status. Despite recommendations that all breastfeeding women use iodine supplements, supplement use was low (only 40%). Further work is require to investigate if current iodine status affects thyroid function. Alternative strategies are also required to ensure breastfeeding women and their infants have adequate iodine.
A phase 1, single-blind, placebo-controlled, 3-arm cross-over trial assessing the appetite enhancing effects of potentially ghrelinergic dairy-derived peptides
Authors: Cushen Samantha J. , Sullivan Erin Stella , Kelly Tracey , Daly Louise E. , Bhuachalla Éadaoin B Ní , Howick Ken , Schellekens Harriët , Cryan John F. , Griffin Brendan T. , Dahly Darren , Ryan Aoife M
Published: June 2020

Methods to stimulate appetite in the sick or elderly remains a challenge with few safe therapeutic options. Ghrelin is an orexigenic hormone, increasing appetite and subsequent food intake. It has received considerable attention as a therapeutic target to stimulate food intake in patients with anorexia. The identification of food-grade bioactives with proven orexigenic effects would mark significant progress in the treatment of disease-related malnutrition. This study therefore investigated the effects of two milk-derived ghrelinergic peptides on appetite and energy intake in healthy humans.

A single-blind, placebo-controlled, 3-arm (placebo, casein bioactive MF1145 and whey bioactive UL-2-141) cross-over trial was conducted in healthy male volunteers. Participants received 26 mg/kg of both the bioactives and placebo. The main outcome measures were energy & protein intake from a set breakfast and ad libitum lunch and subjective appetite sensations as assessed by visual analogue scale (VAS). Basal and postprandial levels of active ghrelin (AG) were measured. Dietary intakes were analysed using Nutritics software. Statistical analyses were performed in R.

Overall, 22 male participants (mean age 27 years) were included, average BMI was 24.6 kg/m2, (19.8 to 30.2 kg/m2). Mean energy and protein intakes at lunch when treated with placebo were 1343 kcal (95% CI: 1215–1471 kcal) and 74 g (95% CI: 66–81 g), respectively. Energy and protein intakes were not significantly different from placebo for either treatment (p = 0.918, p = 0.319 for UL-2-141 and p = 0.889, p = 0.959 for MF1145, respectively). Similarly, appetite, hunger and satiety responses on VAS were not significantly different from placebo for either treatment. AG peak post-lunch on placebo was 653 pg/ml (95% CI: 511–794 pg/ml). Treatment with UL-2-141 resulted in 139 pg/ml reduction in post-prandial AG compared to placebo and treatment with MF1145 resulted in 114 pg/ml reduction compared to placebo. This pattern was significant for both treatments (p = 0.021 and p = 0.045, respectively) however when controlling for fasting-AG, the pattern was no longer significant (p = 0.590 and p = 0.877 respectively). Pre-prandial AG peaks were not significantly different across treatments.

While these peptides have previously demonstrated ghrelinergic effects in rats, no effect on appetite or food intake in humans was identified by this study. This may be attributable to the small sample size or low dose. However, since healthy adults are often not in tune with their own physiological hunger, they may not respond strongly to simple physiological modulators and repeating the study in subjects with established anorexia may be prudent.
Examining the nutritional quality of the product portfolios of major packaged food and beverage companies in Canada
Authors: Vergeer Laura , Ahmed Mavra , Vanderlee Lana , L'Abbé Mary
Published: June 2020

Canada's food supply is abundant in energy-dense products containing excess amounts of sodium, saturated fat and free sugars, increasing Canadians' risk of developing obesity and non-communicable diseases. Food companies shape the food supply through their control over the formulation of their products; however, no studies have examined the healthfulness of products offered by different companies in Canada. This study aimed to assess and compare the nutritional quality of the product portfolios of major packaged food and beverage companies in Canada. Twenty-two top food companies were selected for study, representing a combined 50% and 73% of Canadian packaged food and beverage sales in 2018, respectively. This included 18 multinational companies, 2 Canadian manufacturers and 2 retailers with private-label brands. Nutritional information for products was sourced from the University of Toronto Food Label Information Program 2017 database. The nutritional quality of all products offered by the sampled companies that were included in the database (n = 8,211) were evaluated using the Health Star Rating (HSR) system, with HSRs ranging from 0.5 (less healthy) to 5 (healthier). Descriptive analyses and analysis of variance with post-hoc tests examined the HSRs of each company's products overall and by food category (n = 24). Mean HSRs of companies’ overall product portfolios ranged from 1.8 to 3.7 (μx̅ = 2.7, σx̅ = 0.5) and differed significantly between companies (p < 0.001). Mean HSRs differed between companies for all food categories except eggs (p = 0.5), seafood (p = 0.2), legumes (p = 0.1), nuts and seeds (p = 0.4), and vegetables (p = 0.08). Variation in mean HSRs of products offered by different companies was greatest for beverages (range = 1.3–5.0, μx̅ = 2.0, σx̅ = 1.0), fats/oils (range = 0.7–4.4, μx̅ = 3.6, σx̅ = 1.6), fruit/fruit juices (range = 0.8–4.0, μx̅ = 2.6, σx̅ = 0.9), and sauces/dips/gravies/condiments (range = 0.5–3.4, μx̅ = 2.3, σx̅ = 1.0). These findings demonstrate that the nutritional quality of products offered by leading food manufacturers in Canada varies significantly overall and by food category, with many of these products considered less healthy according to the HSR system. Differences between companies may reflect the nature of their products; for example, products offered by dairy companies were healthier than those of confectionary and soft drink manufacturers, on average. Variation in nutritional quality within food categories illustrates the need and potential for many companies to improve the healthfulness of their products. By identifying companies that offer less healthy products compared with others in Canada, this study may prompt reformulation.