bluuebunny/crossref_metadata_2025 huggingface dataset:
Bridging Perspectives: Exploring the Convergence of Clinimetrics and Network Theory in Mental Health Assessment and Conceptualization
Authors: Tomba Elena , Tomei Giuliano
Published: March 2024

Background: Clinimetrics and network analysis are two methodological approaches that, despite different origins, share the goal of improving mental health clinical assessment beyond the limitations of classical psychometrics. Despite their common goal and comparable conceptualization of clinical assessment, the potential connection and integration between these approaches has not been explored. The aim of this review is to identify meeting points for the potential integration of clinimetrics and network theory. Methods: A literature review was conducted by examining key works in clinimetrics and network theory and comparing similar concepts from the two approaches. Results: Two main areas of theoretical and methodological convergence and complementarity between clinimetrics and network theory were identified, as follows: the characteristics of clinical indexes and the strategies to assess and organize complex clinical data. These topics encompassed sub-topics related to the influence of individual symptoms on clinical presentation, longitudinal assessment of conditions, influence of comorbidities, and standardized procedures for case formulation. Conclusions: Results provide an indication of the potential for integration for these approaches in a single, clinically oriented methodology for psychological and psychiatric illness conceptualization and assessment. Despite the literature search strategy limitations, the results provide a basis for further exploring the potential for developing an integrated methodology for clinical assessment and treatment planning.
An Application of 3D Cross-Well Elastic Reverse Time Migration Imaging Based on the Multi-Wave and Multi-Component Technique in Coastal Engineering Exploration
Authors: Peng Daicheng , Cheng Fei , Xu Hao , Zong Yuquan
Published: March 2024

Precise surveys are indispensable in coastal engineering projects. The extensive presence of sand in the coastal area leads to significant attenuation of seismic waves within unsaturated loose sediments. As a result, it becomes challenging for seismic waves to penetrate the weathered zone and reach the desired depth with significant amount of energy. In this study, the application of three-dimensional (3D) cross-well elastic reverse time migration (RTM) imaging based on multi-wave and multi-component techniques in coastal engineering exploration is explored. Accurate decomposition of vector compressional (P) and shear (S) waves is achieved through two wavefield decoupling algorithms without any amplitude and phase distortion. Additionally, compressional wave pressure components are obtained, which facilitates subsequent independent imaging. This study discusses and analyzes the imaging results of four imaging strategies under cross-correlation imaging conditions in RTM imaging. The analysis leads to the conclusion that scalarizing vector wavefields imaging yields superior imaging of P- and S-waves. Furthermore, the imaging results obtained through this approach are of great physical significance. In order to validate the efficacy of this method in 3D geological structure imaging in coastal areas, RTM imaging experiments were performed on two representative models. The results indicate that the proposed 3D elastic wave imaging method effectively generates accurate 3D cross-well imaging of P- and S-waves. This method utilizes the multi-wave and multi-component elastic wave RTM imaging technique to effectively leverage the Earth’s elastic medium without increasing costs. It provides valuable information about the distribution of subsurface rock layers, interfaces, and other structures in coastal engineering projects. Importantly, this can be achieved without resorting to extensive excavation or drilling operations. This method addresses the limitations of current cross-well imaging techniques, thereby providing abundant and accurate geological and geophysical information for the analysis and interpretation of 3D geological structures in coastal engineering projects. It has important theoretical and practical significance in real-world production, as well as for the study of geological structures in coastal engineering.
Reply to Navarro-Fernandez, I. Nailing down Clinical Nuances. Comment on “Crotti et al. A Terbinafine Sensitive Trichophyton indotineae Strain in Italy: The First Clinical Case of tinea corporis and onychomycosis. J. Fungi 2023, 9, 865”
Authors: Crotti Silvia , Cruciani Deborah , Papini Manuela
Published: March 2024

We have read with interest the comments and observations made regarding the paper “A terbinafine sensitive Trichophyton indotineae strain in Italy: the first clinical case of tinea corporis and onychomycosis” [...]
More Inclusive and Wider Sources: A Comparative Analysis of Data and Political Journalists on Twitter (Now X) in Germany
Authors: Witzenberger Benedict , Pfeffer Jürgen
Published: March 2024

Women are underrepresented in many areas of journalistic newsrooms. In this paper, we examine if this established effect persists in the new forms of journalistic communication, namely social media networks. We use mentions, retweets, and hashtags as measures of journalistic amplification and legitimation. Furthermore, we compare two groups of journalists in different stages of development: political and data journalists in Germany in 2021. Our results show that journalists identified as women tend to favor other women journalists in mentions and retweets on Twitter (now called X), compared to men. While both professions are dominated by men, with a high share of tweets authored by men, women mention and retweet other women more than their male colleagues. Female data journalists also leverage different sources than men. In addition, we found data journalists to be more inclusive of non-member sources in their networks compared to political journalists.
Exploring Sensitivity of Phenology to Seasonal Climate Differences in Temperate Grasslands of China Based on Normalized Difference Vegetation Index
Authors: Wei Xiaoshuai , Xu Mingze , Zhao Hongxian , Liu Xinyue , Guo Zifan , Li Xinhao , Zha Tianshan
Published: March 2024

The affiliation between vegetation phenology and seasonal climate (start and end times of the growing season, or SOS and EOS) provides a basis for acquiring insight into the dynamic response of terrestrial ecosystems to the effects of climate change. Although climate warming is an important factor affecting the advancement or delay of plant phenology, understanding the sensitivity of phenology to seasonal variation in climate factors (e.g., local air temperature, precipitation) is generally lacking under different climate backgrounds. In this study, we investigated the interannual variability of grassland phenology and its spatial variation in temperate regions of China based on satellite-derived products for the normalized difference vegetation index (NDVI) and weather data acquired from 2001 to 2020. We found that due to differences in local climate conditions, the effects of seasonal warming and precipitation on phenology were divergent or even opposite during the 20 years. The sensitivities of the start of growing season (SOS) to both spring temperature and last-winter precipitation was controlled by mean annual precipitation in terms of spatial variation. The SOS in the semi-humid (200–400 mm) region was most sensitive to spring temperature, advancing 5.24 days for each 1 °C rise in the average spring temperature (p < 0.05), while it was most sensitive to last-winter precipitation in arid regions (<200 mm), with SOS advancing up to 2.23 days for every 1 mm increase in the last-winter precipitation (p < 0.05). The end of growing season (EOS) was sensitive to autumn temperature, being delayed 10.13 days for each 1 °C rise in the average autumn temperature in regions with temperatures between −10 °C and −5 °C (p < 0.05). The uncertainty in the determination of the EOS could conceivably be greater than the determination of the SOS due to the dual effects of pre-autumn climate and growth constraints induced by declining fall temperatures. The effect of atmospheric warming on grassland phenology was lessened with increased atmospheric and soil aridity, suggesting that the interaction of regional drought and climate warming is an important source for local-to-regional differences and uncertainties in grass phenological response.
Anti-Swaying Control Strategy of Ship-Mounted 3-RCU Parallel Platform Based on Dynamic Gravity Compensation
Authors: Lv Zhiyuan , Liu Pengfei , Ning Donghong , Wang Shuqing
Published: March 2024

It is essential to ensure stability during marine transportation or the installation of high center of gravity loads. The heavy loads increase gravity disturbance, affecting the steady-state-error control of the multiple degrees of freedom (DOFs) motion compensation platform. In this paper, we propose a proportional derivative (PD) controller with dynamic gravity compensation (PDGC) for a 3-RCU (revolute–cylindrical–universal) parallel platform to improve the control effect of marine motion compensation for high center of gravity loads. We introduce an evaluation parameter of load stability and a weighting coefficient of anti-swaying control to tune the controller performance. The controller can set its control target between the two, keeping the load contact surface level and allowing the load center of gravity with the least movement. By deriving the Jacobian matrix, the gravity disturbance in the joint space is calculated and is compensated in the controller. First, we verify the control superiority of this controller over the PD controller under sinusoidal excitation in simulation and validate the effectiveness of the proposed anti-swing strategy. Then, the experiments are conducted with random excitation. The root mean square (RMS) value of the load’s residual angle with the proposed controller is reduced to 32.2% and 17.6% in two directions, respectively, compared with the PD controller under class 4 sea state excitation. The proposed method is effective for the anti-swaying control of ship-mounted 3-RCU parallel platforms.
Predicting Fan Attendance at Mega Sports Events—A Machine Learning Approach: A Case Study of the FIFA World Cup Qatar 2022
Authors: Al-Buenain Ahmad , Haouari Mohamed , Jacob Jithu Reji
Published: March 2024

Mega sports events generate significant media coverage and have a considerable economic impact on the host cities. Organizing such events is a complex task that requires extensive planning. The success of these events hinges on the attendees’ satisfaction. Therefore, accurately predicting the number of fans from each country is essential for the organizers to optimize planning and ensure a positive experience. This study aims to introduce a new application for machine learning in order to accurately predict the number of attendees. The model is developed using attendance data from the FIFA World Cup (FWC) Russia 2018 to forecast the FWC Qatar 2022 attendance. Stochastic gradient descent (SGD) was found to be the top-performing algorithm, achieving an R2 metric of 0.633 in an Auto-Sklearn experiment that considered a total of 2523 models. After a thorough analysis of the result, it was found that team qualification has the highest impact on attendance. Other factors such as distance, number of expatriates in the host country, and socio-geopolitical factors have a considerable influence on visitor counts. Although the model produces good results, with ML it is always recommended to have more data inputs. Therefore, using previous tournament data has the potential to increase the accuracy of the results.
An Intra-Hospital Spread of Colistin-Resistant K. pneumoniae Isolates—Epidemiological, Clinical, and Genetic Analysis
Authors: Popivanov Georgi , Markovska Rumyana , Gergova Ivanka , Konaktchieva Marina , Cirocchi Roberto , Kjossev Kirien , Mutafchiyski Ventsislav
Published: March 2024

Background and Objective: Klebsiella pneumoniae appears to be a significant problem due to its ability to accumulate antibiotic-resistance genes. After 2013, alarming colistin resistance rates among carbapenem-resistant K. pneumoniae have been reported in the Balkans. The study aims to perform an epidemiological, clinical, and genetic analysis of a local outbreak of COLr CR-Kp. Material and Methods: All carbapenem-resistant and colistin-resistant K. pneumoniae isolates observed among patients in the ICU unit of Military Medical Academy, Sofia, from 1 January to 31 October 2023, were included. The results were analyzed according to the EUCAST criteria. All isolates were screened for blaVIM, blaIMP, blaKPC, blaNDM, and blaOXA-48. Genetic similarity was determined using the Dice coefficient as a similarity measure and the unweighted pair group method with arithmetic mean (UPGMA). mgrB genes and plasmid-mediated colistin resistance determinants (mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5) were investigated. Results: There was a total of 379 multidrug-resistant K. pneumoniae isolates, 88% of which were carbapenem-resistant. Of these, there were nine (2.7%) colistin-resistant isolates in six patients. A time and space cluster for five patients was found. Epidemiology typing showed that two isolates belonged to clone A (pts. 1, 5) and the rest to clone B (pts. 2–4) with 69% similarity. Clone A isolates were coproducers of blaNDM-like and blaOXA-48-like and had mgrB-mediated colistin resistance (40%). Clone B isolates had only blaOXA-48-like and intact mgrB genes. All isolates were negative for mcr-1, -2, -3, -4, and -5 genes. Conclusions: The study describes a within-hospital spread of two clones of COLr CR-Kp with a 60% mortality rate. Clone A isolates were coproducers of NDM-like and OXA-48-like enzymes and had mgrB-mediated colistin resistance. Clone B isolates had only OXA-48-like enzymes and intact mgrB genes. No plasmid-mediated resistance was found. The extremely high mortality rate and limited treatment options warrant strict measures to prevent outbreaks.
Can MRI Accurately Diagnose and Stage Endometrial Adenocarcinoma?
Authors: Rizescu Ramona-Andreea , Sălcianu Iulia Alecsandra , Șerbănoiu Alexandru , Ion Radu Tudor , Florescu Lucian Mihai , Gheonea Ioana-Andreea , Iana Gheorghe , Bratu Ana Magdalena
Published: March 2024

Background and Objectives: Endometrial carcinoma is one of the most common gynecological cancers, and benign lesions such as endometrial hyperplasia, polyps, adenomyosis and leiomyomas should be included in the differential diagnosis. Magnetic resonance imaging has an important role in evaluating endometrial cancer and assessing the depth of myometrial invasion, and it closely correlates with the prognosis of the patient. The purpose of this study is to evaluate the MRI semiology of the endometrial carcinomas that mimic benign lesions, the main factors that may affect the correct diagnosis and the feasibility of magnetic resonance imaging to evaluate the depth of the myometrial invasion of endometrial cancer. Materials and Methods: This is a retrospective analysis of 45 patients that underwent MRI examinations and the lesions were pathologically diagnosed as endometrial carcinoma after surgical resection. This study evaluated the staging accuracy of T2-weighted imaging, diffusion-weighted imaging (DWI), ADC mapping and T1-weighted imaging with fat saturation before and after gadolinium injection. Results: In 36 of the 45 cases, the MRI of the lesion showed the characteristics of endometrial cancer and the diagnosis was certain. Nine lesions (20%) were described as unequivocal and had unspecific MR appearance. In eight of the nine cases (89%), the histopathologic report revealed the presence of leiomyomas and two of these cases (22%) were also associated with adenomyosis. The cause of underestimation in these patients was coexisting lesions exhibiting heterogenous intensity and contrast enhancement, which made it difficult to detect the margins of the lesions. The depth of the myometrial invasion was underestimated in nine cases and overestimated in three cases. The staging accuracy with MRI was 74%. There was a significant correlation between MR imaging and histopathologic finding in the assessment of myometrial invasion (p < 0.001). Cervical extension was noted in eight cases (18%), but was missed on MR imaging in two patients and overstaged in none. Six of them were associated with myometrial invasion in more than 50% of the thickness. There was a significant correlation between MR imaging and histopathologic finding in the assessment of cervical extension (p < 0.001). Conclusions: Our data confirm the high accuracy of MRI in the diagnosis and local staging of endometrial carcinoma. The information provided by MRI has an important role in planning the treatment and the prognosis of the patients.
Ovarian Cancer and the Microbiome: Connecting the Dots for Early Diagnosis and Therapeutic Innovations—A Review
Authors: Choi Seo-Yoon , Choi Jung-Hye
Published: March 2024

Ovarian cancer, which ranks eighth among global female cancers and fifth in fatality, poses a significant health challenge owing to its asymptomatic early stages. Understanding the pathogenesis requires extensive research. Recent studies have emphasized the role of the gut and cervicovaginal microbiota in ovarian cancer. This review explores the current understanding of the relationship between the microbiome and ovarian cancer, considering the potential of biomarkers in the serum and various tissues. Insights into the influence of the microbiome on treatments, including surgery and chemotherapy, open doors to innovative approaches, such as fecal microbiome transplantation. This synthesis of recent findings provides crucial insights into the intricate interplay between the microbiome and ovarian cancer, thereby shaping diagnostic and treatment strategies.
Development of Structure–Property Relationships for Ammonium Transport through Charged Organogels
Authors: Bachmann Adam L. , Hunter Brock , Beckingham Bryan S.
Published: March 2024

Ammonia is a promising carbon-free fuel, but current methods to produce ammonia are energy intensive. New methods are thereby needed, with one promising method being electrochemical nitrogen reduction cells. Efficient cell operation requires robust catalysts but also efficient membrane separators that permit the selective transport of ions while minimizing the transport of the products across the cell. Commercial membranes have an unknown morphology which makes designing improved cells challenging. To address this problem, we synthesized a series of membranes with controlled crosslinking density and chemical composition to understand their impact on ammonium transport. Higher crosslinking density led to lower ammonium permeability. At the highest crosslinking density, similar ammonium permeability was observed independent of the water volume fraction and hydrophobicity of the monomers. These results suggest new directions to develop membranes with reduced ammonium crossover to improve the efficiency of these electrochemical cells.
Metagenomic Insight into the Effect of Probiotics on Nitrogen Cycle in the Coilia nasus Aquaculture Pond Water
Authors: Mang Qi , Gao Jun , Li Quanjie , Sun Yi , Xu Gangchun , Xu Pao
Published: March 2024

Recently, probiotics have been widely applied for the in situ remediation of aquatic water. Numerous studies have proved that probiotics can regulate water quality by improving the microbial community. Nitrogen cycling, induced by microorganisms, is a crucial process for maintaining the balance of the aquatic ecosystem. Nevertheless, the underlying mechanisms by which probiotics enhance water quality in aquatic systems remain poorly understood. To explore the water quality indicators and their correlation with nitrogen cycling-related functional genes, metagenomic analysis of element cycling was performed to identify nitrogen cycling-related functional genes in Coilia nasus aquatic water between the control group (C) and the groups supplemented with probiotics in feed (PF) or water (PW). The results showed that adding probiotics to the aquatic water could reduce the concentrations of ammonia nitrogen (NH4+-N), nitrite (NO2−-N), and total nitrogen (TN) in the water. Community structure analysis revealed that the relative abundance of Verrucomicrobiota was increased from 30 d to 120 d (2.61% to 6.35%) in the PW group, while the relative abundance of Cyanobacteria was decreased from 30 d to 120 d (5.66% to 1.77%). We constructed a nitrogen cycling pathway diagram for C. nasus aquaculture ponds. The nitrogen cycle functional analysis showed that adding probiotics to the water could increase the relative abundance of the amoC_B and hao (Nitrification pathways) and the nirS and nosZ (Denitrification pathways). Correlation analysis revealed that NH4+-N was significantly negatively correlated with Limnohabitans, Sediminibacterium, and Algoriphagus, while NO2−-N was significantly negatively correlated with Roseomonas and Rubrivivax. Our study demonstrated that adding probiotics to the water can promote nitrogen element conversion and migration, facilitate nitrogen cycling, benefit ecological environment protection, and remove nitrogen-containing compounds in aquaculture systems by altering the relative abundance of nitrogen cycling-related functional genes and microorganisms.
Rare Plasmid-Mediated AmpC Beta-Lactamase DHA-1 Located on Easy Mobilized IS26-Related Genetic Element Detected in Escherichia coli from Livestock and Food in Germany
Authors: Manfreda Chiara , Kaesbohrer Annemarie , Schmoger Silvia , Skladnikiewicz-Ziemer Tanja , Grobbel Mirjam , Irrgang Alexandra
Published: March 2024

AmpC beta-lactamases cause resistance to third-generation cephalosporins, including beta-lactamase inhibitors. In Escherichia coli from the German food production chain, the majority of AmpC beta-lactamase activity can be attributed to plasmid-mediated CMY-2 or overproduction of chromosomal AmpC beta-lactamase, but occasionally other enzymes like DHA-1 are involved. This study investigated the prevalence of the AmpC beta-lactamase DHA-1 in ESBL/AmpC-producing E. coli (n = 4706) collected between 2016 and 2021 as part of a German antimicrobial resistance monitoring program along the food chain. Eight isolates (prevalence < 0.2%) were detected and further characterized by PFGE, transformation and conjugation experiments as well as short-read and long-read sequencing. All eight strains harbored blaDHA-1 together with qnrB4, sul1 and mph(A) resistance genes on an IS26 composite transposon on self-transferable IncFII or IncFIA/FIB/II plasmids. During laboratory experiments, activation of the translocatable unit of IS26-bound structures was observed. This was shown by the variability of plasmid sizes in original isolates, transconjugants or transferred plasmids, and correspondingly, duplications of resistance fragments were found in long-read sequencing. This activation could be artificial due to laboratory handling or naturally occurring. Nevertheless, DHA-1 is a rare AmpC beta-lactamase in livestock and food in Germany, and its dissemination will be monitored in the future.
Gold Migration and Precipitation as Collaurum in Orogenic Gold Deposits: Constrains from Microscopic Gold Particles Observed in the Alteration Zone in Shanggong Gold Ore, Henan, China
Authors: Qiao Yu , Han Zhixuan , Zhang Bimin , Wei Xiaocheng , Dong Chunfang , Liu Hanliang
Published: March 2024

Aqueous complexation has long been considered the only viable means of transporting gold to depositional sites in hydrothermal ore-forming systems. Here, we present direct evidence supporting an alternative hypothesis, namely, the transport of gold as colloidal particles. We observed nano-scale gold particles adsorbed on halloysite and micro-scale gold particles in altered rocks by TEM and SEM in the Shanggong orogenic gold deposit. Based on this evidence, we propose a feasible model for the origin of microscopic gold particles in alteration zones. In the early stage of ore-forming fluid, gold may migrate in the form of collaurum, which is maintained by supercritical CO2 and colloidal silica. Low salinity and high pressure are conducive to the stable migration of colloidal gold. When the physicochemical conditions change, some collaurum is precipitated and adsorbed by the clay minerals produced by hydrothermal alteration, and some collaurum undergoes growth and evolves into micro-submicrometer-sized gold particles. This study highlighted the significance of collaurum in the formation of orogenic gold deposits.
Computational Modelling of Intra-Module Connections and Their Influence on the Robustness of a Steel Corner-Supported Volumetric Module
Authors: Heng Si Hwa , Hyland David , Hough Michael , McCrum Daniel
Published: March 2024

This paper investigates the robustness of a single 3D volumetric corner-supported module made of square hollow-section (SHS) columns. Typically, the moment–rotation (M-θ) behaviour of connections within the module (intra-module) is assumed to be fully rigid rather than semi-rigid, resulting in inaccurate assessment (i.e., overestimated vertical stiffness) during extreme loading events, such as progressive collapse. The intra-module connections are not capable of rigidly transferring the moment from the beams to the SHS columns. In this paper, a computationally intensive shell element model (SEM) of the module frame is created. The M-θ relationship of the intra-module connections in the SEM is firstly validated against test results by others and then replicated in a new simplified phenomenological beam element model (BEM), using nonlinear spring elements to capture the M-θ relationship. Comparing the structural behaviour of the SEM and BEM, under notional support removal, shows that the proposed BEM with semi-rigid connections (SR-BEM) agrees well with the validated SEM and requires substantially lower modelling time (98.7% lower) and computational effort (97.4% less RAM). When compared to a BEM with the typically modelled fully rigid intra-module connections (FR-BEM), the vertical displacement in the SR-BEM is at least 16% higher. The results demonstrate the importance of an accurate assessment of framing rotational stiffness and the benefits of a computationally efficient model.
Hybrid Peptide-Alkoxyamine Drugs: A Strategy for the Development of a New Family of Antiplasmodial Drugs
Authors: Embo-Ibouanga Ange W. , Nguyen Michel , Paloque Lucie , Coustets Mathilde , Joly Jean-Patrick , Augereau Jean-Michel , Vanthuyne Nicolas , Bikanga Raphaël , Coquin Naomie , Robert Anne , Audran Gérard , Boissier Jérôme , Mellet Philippe , Benoit-Vical Françoise , Marque Sylvain R. A.
Published: March 2024

The emergence and spread of drug-resistant Plasmodium falciparum parasites shed a serious concern on the worldwide control of malaria, the most important tropical disease in terms of mortality and morbidity. This situation has led us to consider the use of peptide-alkoxyamine derivatives as new antiplasmodial prodrugs that could potentially be efficient in the fight against resistant malaria parasites. Indeed, the peptide tag of the prodrug has been designed to be hydrolysed by parasite digestive proteases to afford highly labile alkoxyamines drugs, which spontaneously and instantaneously homolyse into two free radicals, one of which is expected to be active against P. falciparum. Since the parasite enzymes should trigger the production of the active drug in the parasite’s food vacuoles, our approach is summarized as “to dig its grave with its fork”. However, despite promising sub-micromolar IC50 values in the classical chemosensitivity assay, more in-depth tests evidenced that the anti-parasite activity of these compounds could be due to their cytostatic activity rather than a truly anti-parasitic profile, demonstrating that the antiplasmodial activity cannot be based only on measuring antiproliferative activity. It is therefore imperative to distinguish, with appropriate tests, a genuinely parasiticidal activity from a cytostatic activity.
A Chiral-LC-MS Method for the Simultaneous Quantification of Short-Chain Fatty Acids and D/L-Lactate in the Ruminal Fluid of Dairy Cows
Authors: Liu Zhiqian , Williams S. Richard O. , Jacobs Joe L. , Neachtain Aodan S. O. , Rochfort Simone
Published: March 2024

Short-chain fatty acids (SCFA) and lactate in ruminal fluid are products resulting from the microbial fermentation of substrates and can be used to reflect the composition and activity of the ruminal microbiome. Determination of SCFA and D-/L-lactate in ruminal fluid currently requires two separate protocols, which is time-consuming and costly. In this study, we have optimised and validated a simple and unified 3-nitrophenylhydrazine (3-NPH) derivatisation protocol and a 20 min chiral-LC-MS method for the simultaneous quantification of all SCFA and D- and L-lactate in ruminal fluid. This method, which requires no sample pretreatment or purification shows adequate sensitivity (limit of detection (LOD): 0.01 µg/mL), satisfactory accuracy (recovery: 88–103%), and excellent reproducibility (relative standard deviation (RSD) for repeated analyses < 3% for most analytes). The application of this method to a cohort of 24 animals allowed us to reveal a large inter-cow variation in ruminal SCFA and lactate level, the concentration range for each species, the widespread correlation between different SCFA, and the strong correlation between D- and L-lactate.
Effect of Deashing Treatment on Ash Fusion Characteristics of Biochar from Bamboo Shoot Shells
Authors: Ren Hao , Gao Qi , Ni Liangmeng , Su Mengfu , Rong Shaowen , Liu Shushu , Zhong Yanhang , Liu Zhijia
Published: March 2024

To investigate the influence of deashing on fusion characteristics, a combined method of water and acid washing with different sequences (water washing followed by acid washing, and acid washing followed by water washing) was used to treat the biochar of bamboo shoot shells (BBSSs). The results show that deashing decreased the K content of the biochar from 50.3% to 1.08% but increased the Si content from 33.48% to 89.15%. The formation of silicates and aluminosilicates from alkali metal oxides with silicon was an inevitable result of ash phase transformation at the high temperatures used to improve the fusion temperature (>1450 °C). The thermochemical behavior of ash mainly occurs at 1000 °C. The deashing treatment significantly reduced the reaction intensity during the high-temperature process. This significantly increased the thermal stability of the ash. The adjustment of the washing sequence had a slight impact on the chemical compositions, but the differences in ash micromorphology were obvious. Deashing treatments with different washing sequences can significantly improve ash fusion properties effectively and reduce the risk of scaling, slagging, and corrosion. This study provides a new and reasonable strategy for the deashing of biochar to commercially utilize bamboo shoot shell resources.
Enzymatic Hydrolysis Optimization of Yak Whey Protein Concentrates and Bioactivity Evaluation of the Ultrafiltered Peptide Fractions
Authors: Hao Lingshen , Li Xuefei , Zhao Baotang , Song Xuemei , Zhang Yan , Liang Qi
Published: March 2024

Yak whey protein concentrates (YWPCs) have good functional properties, but there is still a gap in the study of their peptides. In this study, peptides were obtained by enzymatic hydrolysis, and the bioactivity of each ultrafiltration fraction was evaluated using an optimal process. YWPCs were isolated and purified from yak milk as the raw material. Alkaline protease, trypsin, and papain were used to hydrolyze YWPCs. The protease with the highest degree of hydrolysis (DH) and peptide concentration was selected as the most suitable enzyme. The effects of pH, temperature, time, and the enzyme-to-substrate ratio (E/S) on the DH and peptide concentration were investigated, and response surface methodology was utilized to optimize the hydrolysis process. The hydrolysate was separated using ultrafiltration membranes with molecular weight cut-offs of 10 kDa, 5 kDa, 3 kDa, and 1 kDa. The bioactivity of each ultrafiltration component was analyzed, including the inhibition rates of α-amylase and xanthine oxidase (XOD) activities and the scavenging rates of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radicals. The results indicated that alkaline protease was the best enzyme for hydrolyzing YWPCs. The peptide concentration in the YWPC hydrolysate was the highest (17.21 mg/mL) at a pH of 8 and a concentration of 7500 U/g, after 2.5 h at 62 °C. The enzymatic hydrolysate was ultrafiltered to yield four peptide fractions, of which the <1 kDa peptides exhibited the highest α-amylase inhibitory activity (22.06%), XOD inhibitory activity (17.15%), and ABTS cationic free radical scavenging rate (69.55%). This demonstrates the potential of YWPC hydrolyzed peptides for hypoglycemic, uric acid-lowering, and antioxidant applications, providing a theoretical basis for the high-value utilization of YWPCs.
Composite of KLVFF-Transthyretin-Penetratin and Manganese Dioxide Nanoclusters: A Multifunctional Agent against Alzheimer’s β-Amyloid Fibrillogenesis
Authors: Lan Haitao , Wang Ying , Liu Wei , Dong Xiaoyan , Sun Yan
Published: March 2024

Design of amyloid β-protein (Aβ) inhibitors is considered an effective strategy for the prevention and treatment of Alzheimer’s disease (AD). However, the limited blood–brain barrier (BBB) penetration and poor Aβ-targeting capability restricts the therapeutic efficiency of candidate drugs. Herein, we have proposed to engineer transthyretin (TTR) by fusion of the Aβ-targeting peptide KLVFF and cell-penetrating peptide Penetratin to TTR, and derived a fusion protein, KLVFF-TTR-Penetratin (KTP). Moreover, to introduce the scavenging activity for reactive oxygen species (ROS), a nanocomposite of KTP and manganese dioxide nanoclusters (KTP@MnO2) was fabricated by biomineralization. Results revealed that KTP@MnO2 demonstrated significantly enhanced inhibition on Aβ aggregation as compared to TTR. The inhibitory effect was increased from 18%, 33%, and 49% (10, 25, and 50 μg/mL TTR, respectively) to 52%, 81%, and 100% (10, 25, and 50 μg/mL KTP@MnO2). In addition, KTP@MnO2 could penetrate the BBB and target amyloid plaques. Moreover, multiple ROS, including hydroxyl radicals, superoxide radicals, hydrogen peroxide, and Aβ-induced-ROS, which cannot be scavenged by TTR, were scavenged by KTP@MnO2, thus resulting in the mitigation of cellular oxidative damages. More importantly, cell culture and in vivo experiments with AD nematodes indicated that KTP@MnO2 at 50 μg/mL increased the viability of Aβ-treated cells from 66% to more than 95%, and completely cleared amyloid plaques in AD nematodes and extended their lifespan by 7 d. Overall, despite critical aspects such as the stability, metabolic distribution, long-term biotoxicity, and immunogenicity of the nanocomposites in mammalian models remaining to be investigated, this work has demonstrated the multifunctionality of KTP@MnO2 for targeting Aβ in vivo, and provided new insights into the design of multifunctional nanocomposites of protein–metal clusters against AD.
Self-Powered Broadband Photodetector Based on NiO/Si Heterojunction Incorporating Graphene Transparent Conducting Layer
Authors: Pandit Bhishma , Parida Bhaskar , Jang Hyeon-Sik , Heo Keun
Published: March 2024

In this study, a self-powered broadband photodetector based on graphene/NiO/n-Si was fabricated by the direct spin-coating of nanostructured NiO on the Si substrate. The current–voltage measurement of the NiO/Si heterostructure exhibited rectifying characteristics with enhanced photocurrent under light illumination. Photodetection capability was measured in the range from 300 nm to 800 nm, and a higher photoresponse in the UV region was observed due to the wide bandgap of NiO. The presence of a top graphene transparent conducting electrode further enhanced the responsivity in the whole measured wavelength region from 350 to 800 nm. The photoresponse of the NiO/Si detector at 350 nm was found to increase from 0.0187 to 0.163 A/W at −1 V with the insertion of the graphene top layer. A high photo-to-dark current ratio (≃104) at the zero bias indicates that the device has advantageous application in energy-efficient high-performance broadband photodetectors.
Exploring the Application of Graphene Oxide-Based Nanomaterials in the Repair of Osteoporotic Fractures
Authors: Zhou Hongfa , Chen Jinyuan , Zhang Xuan , Chen JingJing , Chen Jiayou , Jia Shicheng , Wang Deli , Zeng Hui , Weng Jian , Yu Fei
Published: March 2024

Osteoporotic fractures are induced by osteoporosis, which may lead to the degradation of bone tissues and microstructures and impair their healing ability. Conventional internal fixation therapies are ineffective in the treatment of osteoporotic fractures. Hence, developing tissue engineering materials is crucial for repairing osteoporotic fractures. It has been demonstrated that nanomaterials, particularly graphene oxide (GO), possess unique advantages in tissue engineering due to their excellent biocompatibility, mechanical properties, and osteoinductive abilities. Based on that, GO-nanocomposites have garnered significant attention and hold promising prospects for bone repair applications. This paper provides a comprehensive insight into the properties of GO, preparation methods for nanocomposites, advantages of these materials, and relevant mechanisms for osteoporotic fracture applications.
Review of Ground Penetrating Radar Applications for Bridge Infrastructures
Authors: Boldrin Paola , Fornasari Giacomo , Rizzo Enzo
Published: March 2024

Infrastructure bridges play a crucial role in fostering economic and social development. However, the adverse effects of natural hazard and weather degradation, coupled with escalating rates of traffic, pose a significant threat. The resultant strain on the structure can lead to undue stress, elevating the risk of a critical asset failure. Hence, non-destructive testing (NDT) has become indispensable in the surveillance of bridge infrastructure. Its primary objectives include ensuring safety, optimizing structural integrity, minimizing repair costs, and extending the lifespan of bridges. NDT techniques can be applied to both existing and newly constructed bridge structures. However, it is crucial to recognize that each NDT method comes with its own set of advantages and limitations tailored to specific tasks. No single method can provide an effective and unequivocal diagnosis on its own. Among the various NDT methods, Ground Penetrating Radar (GPR) has emerged as one of the most widely employed techniques for monitoring bridges. In fact, recent technical regulations now mandate the use of GPR for bridge monitoring and characterization, underscoring its significance in ensuring the structural health and longevity of these critical infrastructures. Ground Penetrating Radar (GPR) stands out as one of the most highly recommended non-destructive methods, offering an efficient and timely assessment of the structural conditions of infrastructure. Recognizing the pivotal role of non-destructive testing (NDT) in this context, this paper aims to elucidate recent scientific endeavors related to the application of GPR in bridge engineering structures. The exploration will commence with a focus on studies conducted both at the model level within laboratory settings and on real cases. Subsequently, the discussion will extend to encompass the characterization and monitoring of the bridge’s main elements: slab, beam, and pillar. By delving into these scientific experiences, this paper intends to provide valuable insights into the efficacy and applicability of GPR in assessing and ensuring the structural integrity of bridges. This paper provides a concise survey of the existing literature on the application of Ground Penetrating Radar (GPR) in the assessment of bridges and viaducts constructed with masonry and reinforced concrete, taking into account papers of journal articles and proceedings available on open databases. Various approaches employed in both laboratory and field settings will be explored and juxtaposed. Additionally, this paper delves into discussions on novel processing and visualization approaches, shedding light on advancements in techniques for interpreting GPR data in the context of bridge and viaduct evaluations.
Implementation of National Nutrition Policies and Strategies to Reduce Unhealthy Diets: An Ecological Analysis of 194 Countries from 2017 to 2021
Authors: Forray Alina Ioana , Borzan Cristina Maria
Published: March 2024

This study investigates the implementation of national policies and strategies to control unhealthy diets, which are pivotal in the global surge of non-communicable diseases. Leveraging data from the World Health Organization’s Non-Communicable Diseases Progress Monitors and Country Capacity Surveys, we calculated aggregate implementation scores for 13 diet-related policies across 194 countries from 2017 to 2021. We used descriptive statistics and linear regression to investigate the implementation trends and associations between key national-level factors and implementation scores. The mean score in 2021 was 52% (SD = 24), with no statistical differences in the 5-year period. Stark disparities in implementation efficacy were noted, ranging from comprehensive adoption in some nations to minimal application in others. Our analysis also highlights a shifting focus in policy adoption: notably, an increased commitment to taxing sugar-sweetened beverages juxtaposed with a decline in dietary awareness initiatives. Significant predictors of policy implementation include the Human Development Index, the cost of a healthy diet, and health service coverage. These findings suggest a complex interdependence of socioeconomic factors influencing policy implementation. Our research underscores the need for multifaceted, globally collaborative strategies to effectively combat diet-related diseases, emphasizing the importance of comprehensive policy frameworks in public health interventions.
Genistein Supplementation and Bone Health in Breast Cancer in Rats
Authors: Skrajnowska Dorota , Bielecki Wojciech , Szterk Arkadiusz , Ofiara Karol , Bobrowska-Korczak Barbara
Published: March 2024

The aim of our study was to analyse the effect of supplementation with various forms of genistein (nano-, micro-, and macro-) on the mineral status of rat femurs in conditions of DMBA-induced mammary gland neoplasia. Thirty-two 30-day-old Sprague Dawley rats were used in the study. The rats were divided into four experimental groups: a control group (without supplementation) and groups supplemented with nanosized (92 ± 41 nm), microsized (587 ± 83 nm), and macrosized genistein. Micromorphometric and histological examination of the rat femurs were performed, as well as analysis of the weight and mineral composition (17 elements). Quadrupole ICP-MS was used for analysis of all trace elements. Supplementation with genistein (nano-, micro-, and macro-) was shown to cause changes in the mineral composition of the bones. In the rats receiving nanogenistein, disintegration of the bone tissue was observed. The femurs of these animals had higher content of calcium (by nearly 300%) and potassium (by 25%) than the other groups, while the level of magnesium was about 22% lower. In the case of microelements, there were increases in copper (by 67%), boron (48%), manganese (13%), and nickel (100%), and a 16% decrease in strontium compared to the bones of rats without genistein supplementation. Changes in micromorphometric parameters, resulting in increased bone fragility, were observed. Administration of genistein was found to have an effect on the amount of trace elements in the bone tissue of rats with breast cancer.
Targeting the Gut: A Systematic Review of Specific Drug Nanocarriers
Authors: Garbati Patrizia , Picco Cristiana , Magrassi Raffaella , Signorello Paolo , Cacopardo Ludovica , Dalla Serra Mauro , Faticato Maria Grazia , De Luca Maria , Balestra Francesco , Scavo Maria Principia , Viti Federica
Published: March 2024

The intestine is essential for the modulation of nutrient absorption and the removal of waste. Gut pathologies, such as cancer, inflammatory bowel diseases (IBD), irritable bowel syndrome (IBS), and celiac disease, which extensively impact gut functions, are thus critical for human health. Targeted drug delivery is essential to tackle these diseases, improve therapy efficacy, and minimize side effects. Recent strategies have taken advantage of both active and passive nanocarriers, which are designed to protect the drug until it reaches the correct delivery site and to modulate drug release via the use of different physical–chemical strategies. In this systematic review, we present a literature overview of the different nanocarriers used for drug delivery in a set of chronic intestinal pathologies, highlighting the rationale behind the controlled release of intestinal therapies. The overall aim is to provide the reader with useful information on the current approaches for gut targeting in novel therapeutic strategies.
Polymer-Drug Anti-Thrombogenic and Hemocompatible Coatings as Surface Modifications
Authors: Zawidlak-Węgrzyńska Barbara , Rydz Joanna , Musioł Marta , Radziwon-Balicka Aneta
Published: March 2024

Since the 1960s, efforts have been made to develop new technologies to eliminate the risk of thrombosis in medical devices that come into contact with blood. Preventing thrombosis resulting from the contact of a medical device, such as an implant, with blood is a challenge due to the high mortality rate of patients and the high cost of medical care. To this end, various types of biomaterials coated with polymer-drug layers are being designed to reduce their thrombogenicity and improve their hemocompatibility. This review presents the latest developments in the use of polymer-drug systems to produce anti-thrombogenic surfaces in medical devices in contact with blood, such as stents, catheters, blood pumps, heart valves, artificial lungs, blood vessels, blood oxygenators, and various types of tubing (such as for hemodialysis) as well as microfluidic devices. This paper presents research directions and potential clinical applications, emphasizing the importance of continued progress and innovation in the field.
Maleimide–Thiol Linkages Alter the Biodistribution of SN38 Therapeutic Microbubbles Compared to Biotin–Avidin While Preserving Parity in Tumoral Drug Delivery
Authors: Ingram Nicola , Abou-Saleh Radwa H. , Race Amanda D. , Loadman Paul M. , Bushby Richard J. , Evans Stephen D. , Coletta P. Louise
Published: March 2024

Therapeutic microbubbles (thMBs) contain drug-filled liposomes linked to microbubbles and targeted to vascular proteins. Upon the application of a destructive ultrasound trigger, drug uptake to tumour is improved. However, the structure of thMBs currently uses powerful non-covalent bonding of biotin with avidin-based proteins to link both the liposome to the microbubble (MB) and to bind the targeting antibody to the liposome–MB complex. This linkage is not currently FDA-approved, and therefore, an alternative, maleimide–thiol linkage, that is currently used in antibody–drug conjugates was examined. In a systematic manner, vascular endothelial growth factor receptor 2 (VEGFR2)-targeted MBs and thMBs using both types of linkages were examined for their ability to specifically bind to VEGFR2 in vitro and for their ultrasound imaging properties in vivo. Both showed equivalence in the production of the thMB structure, in vitro specificity of binding and safety profiles. In vivo imaging showed subtle differences for thMBs where biotin thMBs had a faster wash-in rate than thiol thMBs, but thiol thMBs were longer-lived. The drug delivery to tumours was also equivalent, but interestingly, thiol thMBs altered the biodistribution of delivery away from the lungs and towards the liver compared to biotin thMBs, which is an improvement in biosafety.