bluuebunny/crossref_metadata_2025 huggingface dataset:
[Proof Reading] Dengue virus serotype 3 NS2B-NS3 protease fusion construct using parallel rapid protein expression and purification: 100 mL cultures v1
Authors: fairhead michael , Wang Korvus
Published: October 2024

This protocol details the small scale (100mL) expression and purification of Dengue virus serotype 3 NS2B-NS3 protease fusion constructs using the parallel rapid protein expression and purification (PREPX) method. Recombinant proteins are expressed in Escherichia coli using the autoinduction method and then purified in parallel using a IMAC, desalt, tag cleavage, reverse IMAC and gel filtration work flow. This protocol is adapted from the original PREPX protocol, which is suitable for expressing and purifying multiple constructs in parallel. The protocol can also be adapted for single construct.
Dengue virus serotype 3 NS2B-NS3 protease fusion construct using parallel rapid protein expression and purification: 100 mL cultures v2
Authors: fairhead michael , Wang Korvus
Published: October 2024

This protocol details the small scale (100mL) expression and purification of Dengue virus serotype 3 NS2B-NS3 protease fusion constructs using the parallel rapid protein expression and purification (PREPX) method. Recombinant proteins are expressed in Escherichia coli using the autoinduction method and then purified in parallel using a IMAC, desalt, tag cleavage, reverse IMAC and gel filtration work flow. This protocol is adapted from the original PREPX protocol, which is suitable for expressing and purifying multiple constructs in parallel. The protocol can also be adapted for single construct.
Dengue virus serotype 2 NS2B-NS3 protease fusion construct small scale expression and purification protocol v2
Authors: Wang Korvus , fairhead michael , Williams Eleanor
Published: October 2024

This protocol details the co-expression and purification of Dengue virus serotype 2 NS2B-NS3 protease fusion construct bearing a N-terminal His-StrepII tag at small scale (<6L). This protocol produces catalytically active enzyme suitable for biochemical assays. The construct used here is QQ01D2VNS2BA-c001.
Limited Congruence: Citizens’ Attitudes and Party Rhetoric About Referendums and Deliberative Practices
Authors: Gherghina Sergiu , Geissel Brigitte , Henger Fabian
Published: October 2024

Both citizens and political parties refer to novel participatory practices in the contemporary crisis of representative democracy. Survey data indicate a growing demand for such practices within the electorate, while political parties have also begun discussing them more frequently. However, previous studies on citizens’ attitudes and parties’ discourse on democratic innovations rarely speak to each other. It remains unclear whether citizens’ attitudes and parties’ discourse are congruent. This article seeks to address this gap in the literature and analyses the extent to which political parties reflect citizens’ attitudes towards referendums and citizens’ deliberation in their manifestos. We cover 15 political parties in Germany and the UK. Our analysis uses party manifesto data between 2010 and 2024, and data from surveys conducted on national representative samples. Our findings reveal that political parties and citizens rarely have congruent approaches towards referendums and deliberative practices. People’s enthusiasm about referendums is hardly reflected in parties’ rhetoric, but the latter reacts gradually to the public appetite for deliberation. There are visible differences between opposition parties and those in government.
Morphological characterization of glial cells in the Substantia nigra of spontaneously hypertensive SHR rats
Authors: Kulikova Polina V. , Guselnikova Valeria V.
Published: October 2024

BACKGROUND: Substantia nigra is the main dopaminergic center of the brain. Massive loss of dopaminergic neurons is characteristic for Parkinson’s disease. Arterial hypertension is considered a possible risk factor for Parkinson’s disease development, but to date there is no understanding of the mechanisms that may mediate the effect of high blood pressure on the death of dopaminergic neurons and the development of Parkinson’s disease. Since neuroglia is known to make a major contribution to the pathogenesis of neurodegeneration, it is important to evaluate the effect of arterial hypertension on the functional status of glial cells in the Substantia nigra.
AIM: The aim of the study was to analyze the features of morphofunctional state of both microglia and astroglia in the Substantia nigra of spontaneously hypertensive rats.
MATERIALS AND METHODS: Brain samples from Wistar rats (n = 3) and from spontaneously hypertensive SHR rats aged 9 months (n = 4) were used as a material for the study. Brain sections were immunostained for tyrosine hydroxylase to identify the Substantia Nigra, for calcium-binding protein Iba-1 to identify microglia, and for glial fibrillary acidic protein to identify astrocytes.
RESULTS: Compared to the control, microglial cells in the Substantia nigra of spontaneously hypertensive rats show signs of moderate activation manifested in significant thickening of the processes of microgliocytes. Single microgliocytes exhibit ameboid morphology, indicating their strong activation. A close spatial relationship with the bodies of dopaminergic neurons was noted for some identified microgliocytes. Astrocytes in Substantia nigra of both Wistar rats and SHR rats show no signs of activation.
CONCLUSIONS: Arterial hypertension may be one of the causes of neuroinflammation mediated by microglia in the Substantia nigra.
Effective combinations of silver nanoparticles and antimicrobial peptides against antibiotic-resistant bacteria
Authors: Vladimirova Elizaveta V.
Published: October 2024

BACKGROUND: The problem of constantly growing resistance of microorganisms to the antibiotics used forces scientists to constantly search for new antimicrobial agents.
AIM: To study the antimicrobial activity and toxicity of silver nanoparticles and antimicrobial peptides with a β-hairpin structure, and to characterize their combined antimicrobial action.
MATERIALS AND METHODS: The antimicrobial activity of silver nanoparticles and antimicrobial peptides against antibiotic-resistant bacteria was assessed by serial dilutions in a liquid nutrient medium, and the combined antimicrobial activity – by serial dilutions according to the “checkerboard” method. The toxicity of the substances to human cells was determined using a hemolytic test.
RESULTS: The studied silver nanoparticles have pronounced antimicrobial activity and low toxicity to human erythrocytes. Silver nanoparticles containing acetylcysteine are more active against gram-negative bacteria than against gram-positive ones. Synergism of the antibacterial action was detected with the combined use of silver nanoparticles stabilized with sodium oleate and protegrin-1. A synergistic antimicrobial effect was also noted for combinations of silver nanoparticles containing acetylcysteine with protegrin-1, ricaecilin and shuchin-4.
CONCLUSIONS: Effective combinations of silver nanoparticles and antimicrobial peptides have been identified that act synergistically against antibiotic-resistant bacterial strains, i.e. they multiply the effect of each other. These combinations can be used as prototypes for the development of antibacterial drugs.
Reducing binge eating behavior in rats using the novel ghrelin receptor antagonist Agrelax
Authors: Nadbitova Natalia D.
Published: October 2024

BACKGROUND: Binge eating disorder is the most common eating disorder, characterized by recurrent episodes of binge eating during which a person consumes excessive amounts of food in the absence of hunger. These episodes of binge eating are usually accompanied by a feeling of lack of control with an inability to refrain from eating or stop eating once it has started. A rodent model of binge eating disorder shows that intermittent consumption of energy-dense foods induces binge eating behavior independent of weight gain.
AIM: To study the effect of the ghrelin receptor antagonist Agrelax on compulsive overeating in rats.
MATERIALS AND METHODS: The study involved 30 male Wistar rats. To model compulsive overeating, animals received high-calorie food (a mixture based on chocolate-nut butter spread) 3 times a week while maintaining free access to standard pelleted food and water. Compulsivity in behavior was assessed using the pellet burying test. The ghrelin receptor antagonist Agrelax was administered intranasally, 1 µg/1 µl, 10 µl in each nostril for 7 days.
RESULTS: Compulsive behavior was assessed using the pellet burying test. The experimental group of animals receiving a high-calorie diet buried a significantly larger number of pellets than the control group (p 0.01). After a 7-day course of Agrelax, the number of buried balls decreased significantly, reaching the values of the control group (p 0.05). A technique for compulsive overeating in rats was developed by giving high-calorie food 3 times a week. After a 7-day course of Agrelax, the consumption of high-calorie foods significantly decreased (p 0.05). The daily consumption of standard feed in the group did not differ compared to the control group. However, after the course of Agrelax administration, the consumption of standard feed decreased.
CONCLUSIONS: The data obtained suggest new ways of synthesizing peptide pharmacological agents based on ghrelin and its antagonists for the correction of food addiction.
Directed modification of characteristics of syntetyc analogue of proline-rich peptide ChBac3.4 through dendrimerisation
Authors: Galianova Maya V.
Published: October 2024

BACKGROUND: According to the World Health Organization, microbial resistance to antibiotics is one of the most serious threats to human health, food safety and economic development. Antimicrobial peptides of animal origin, to which resistance is difficult to develop, appear to be a promising option to overcome this problem. To solve one of the problems in the practical use of peptides — their rapid degradation in blood plasma — dendrimerization of linear analogues of already existing antimicrobial peptides can be used. This work shows that dendrimers of shortened analogue of the natural proline-rich peptide ChBac3.4 are superior in antimicrobial activity to the original peptide and are much more stable in blood serum. At the same time, as the original peptide, branched analogues do not have hemolytic activity.
AIM: The aim of this study is to develop new structural analogues of a peptide that have improved stability in blood serum while maintaining high antimicrobial activity. To achieve this, we intend to create dendrimeric (branched) molecules by synthesizing peptides using solid-phase methods.
MATERIALS AND METHODS: We will investigate the antimicrobial properties of the peptides using the method of serial dilutions in a liquid nutrient medium against both Gram-positive and Gram-negative bacteria. We will also assess the stability of the peptides by culturing them in the presence of human blood serum.
RESULTS: After making modifications to the original peptide, we expect to see a significant increase in its antimicrobial activity against all strains tested. Additionally, we anticipate that the resistance of these peptides will increase many times over.
CONCLUSIONS: Overall, our findings suggest that dendrimerization can effectively improve the properties of proline-rich peptides, making them more suitable for use in medical applications.
Potential hepatoprotective properties of synthetic derivatives of thiourea — 2-aminobenzothiazole and IEM-2320
Authors: Klimenko Dmitry I. , Demidova Ekaterina O. , Asatryan Tatevik T. , Myznikov Leonid V. , Nadbitova Natalia D. , Matveeva Ekaterina V. , Moskvicheva Sofia V. , Borzykh Sofia A. , Evdokimova Natalia R. , Lukk Margarita V. , Ramsh Stanislav M. , Ganapolsky Vyacheslav P. , Karpova Inessa V.
Published: October 2024

BACKGROUND: Toxic hepatitis is characterized by a significant severity of the course and by high mortality. So, search for effective means of correcting this condition seems to be relevant. It has previously been shown that some thiazolo[5,4-b]indole derivatives are capable to prevent experimental toxic liver damage in laboratory animals. In continuation of this direction, the study of the hepatoprotective properties of synthetic derivatives of thiourea 2-aminobenzothiazole and IEM-2320 was undertaken.
AIM: To study the potential hepatoprotective properties of 2-aminobenzothiazole and IEM-2320 in acute carbon tetrachloride poisoning.
MATERIALS AND METHODS: Experiments were carried out on 30 male white outbred rats (150–270 g). On a model of carbon tetrachloride poisoning (50% solution of CCl4 in vaseline oil, 0.2 ml/100 g of body weight), the potential hepatoprotective properties of 2-aminobenzothiazole (32 mg/kg) and IEM-2320 (20 mg/kg) were investigated. Ademethionine (Heptral, AbbVie, S.r.L, Italy) at a dose of 20 mg/kg was used as a comparison drug. The studied drugs were administered intraperitoneally 4 times: one hour before the introduction of CCl4, and then — for three subsequent days after the poisoning with CCl4. The results were compared with those of 2 control groups. Animals of the 1st control group were not exposed to CCl4, instead of the studied drugs, they were injected with an equivalent volume of saline solution (0.2 ml/100 g of body weight). Animals of the 2nd control group did not receive any treatment after the CCl4 poisoning. Blood was taken on the seventh day of the experiment. The total contents of protein, glucose, and total cholesterol in blood serum were determined, and the activities of alanine aminotransferase and aspartate aminotransferase were evaluated.
RESULTS: Under the CCl4 poisoning, the administration of 2-aminobenzothiazole led to a significant decrease in the concentrations of glucose, total protein, total cholesterol and alanine aminotransferase activity. When IEM-2320 was administered under the condition of poisoning, a decrease in the concentrations of total protein, glucose, urea, total cholesterol, and the alanine aminotransferase activity was observed.
CONCLUSIONS: A significant decrease in the biochemical parameters of blood serum after administration of 2-aminobenzothiazole and IEM-2320 under the condition of CCl4 poisoning demonstrates thir potential hepatoprotective properties under acute hepatotropic poisonings.
The evolution and causes of the change in the demographic model of Russia. Message 1. General assessment of historical, medical, social and demographic events
Authors: Belyakov Nikolay A. , Trofimova Tatyana N. , Ailamazyan Eduard K. , Boeva Ekaterina V. , Vassoevich Andrey L.
Published: October 2024

The demographic situation in different periods was determined by the general patterns of childbearing, epidemics and the state of society. In Russia, even against the background of great upheavals, population growth has been observed for several centuries with a high birth rate, which exceeded the total mortality rate. Epidemics, wars and political events of the twentieth century, as well as the change of priorities in the 1990s, left their mark and led to a decrease in the population. This analytical review is devoted to the evolutionary changes in the Russian population model. The main historical, medical, social and demographic events that dynamically affect the demographic situation in our country are considered. Special attention is paid to the factors influencing the decline in the population in the country, which must be taken into account when planning and implementing state and territorial programs to stimulate the birth rate, taking into account the main political and economic changes.
Artificial intelligence in medicine: neural networks for analyzing systemic hemodynamics
Authors: Sokolova Evgenia A. , Sergeev Timofey V. , Kuropatenko Maria V.
Published: October 2024

Artificial neural networks are capable of efficiently processing large data sets, as well as solving the tasks of prediction, classification and data recovery. The article considers each of the above tasks in detail and studies literature sources devoted to the topic under study. Artificial neural networks cope with the tasks with a high degree of accuracy. The methods of application of neural networks for the analysis of systemic haemodynamics are described. Modern neural networks can analyse medical data and are able to work with incomplete data, find hidden patterns in them, and can be adapted to solve a wide range of problems. Our laboratory is developing an artificial neural network capable of classifying indicators describing the state of haemodynamics of subjects and recovering missing or incomplete data. Thus, artificial neural networks can act as an efficient method of analysing systemic hemodynamic parameters.
Delayed effects of antibiotic therapy in endotoxinemia
Authors: Kukushkina Ekaterina S. , Lebedeva Elena Ya. , Maystrenko Victoria A. , Kudrinskaya Valentina M.
Published: October 2024

BACKGROUND: Nowadays, due to the increasing number of infectious and inflammatory diseases, the problem of the use of antibacterial drugs becomes especially important. As a result of the action of toxins, inflammatory processes can affect the central nervous system with the subsequent development of neuroinflammation. Activation of neuroinflammation leads to dysregulation of many physiological functions. These negative appearances can be observed even after a long period. It is known that doxycycline is a tetracycline-type antibiotic, which is able to penetrate the blood-brain barrier and has anti-inflammatory activity.
AIM: The aim of this study was to investigate the nature of delayed physiological changes in rats against the background of administration of the antibacterial drug doxycycline in the LPS-induced model of neuroinflammation.
MATERIALS AND METHODS: Four groups of Wistar rats, 10 males in each group, were used in the experiment. The first group was injected once intraperitoneally with physiological solution, the second group — with lipopolysaccharide (1 mg/kg). Animals of the third and fourth groups received intragastrically doxycycline solution (25 mg/kg) daily for two weeks. On the 15th day of the experiment, rats from the fourth group were injected with lipopolysaccharide (1 mg/kg). Body weight of animals, mass coefficients of immunocompetent organs, as well as behaviour and motor activity of rats in the “Open Field” test were evaluated at several time points.
RESULTS: It was shown that systemic injection of lipopolysaccharide led to an increase in the mass coefficients of spleen, kidneys and adrenal glands compared to the group of animals receiving doxycycline beforehand. These changes were noted 48 h and 2 months after the injection of endotoxin. In the “Open Field” test, animals that were injected with doxycycline and lipopolysaccharide showed no violations of motor activity and research behavior, unlike the group that received only lipopolysaccharide.
CONCLUSIONS: It can be assumed that the physiological effects of doxycycline in the lipopolysaccharide-induced model of neuroinflammation revealed at early and late terms are not limited to the antibacterial effect of the drug and are mediated by anti-inflammatory and potential neuroprotective effects on the central nervous system.
Activity of protegrin-1 against mouse Ehrlich ascites carcinoma in vitro and in vivo
Authors: Rudel Alena E. , Filatenkova Tatiana A. , Zharkova Maria S.
Published: October 2024

BACKGROUND: The problem of multidrug resistance in cancer treatment creates an urgent demand for developing new effective antitumor agents. Due to the unusual mechanism of recognizing and damaging tumor cells, antimicrobial peptides are considered as possible prototypes for designing such therapeutics.
AIM: This work was aimed to compare the antitumor potential of the promising membranolytic antimicrobial peptide protegrin-1 in vitro and in vivo in Ehrlich ascites carcinoma mice model.
MATERIALS AND METHODS: We used two variants of the model by inducing a tumor in solid or ascites form. In the first case, the mice were injected with the peptide twice a week for three weeks, and in the second injections were provided every other day for six days. The activity of antimicrobial peptides against isolated Ehrlich ascites carcinoma cells in vitro was analyzed using MTT-test.
RESULTS: Protegrin-1 demonstrated high activity against Ehrlich ascites carcinoma cells in vitro, but had no significant effect on the lifespan of mice bearing solid or ascites form of Ehrlich tumor at the dosing and administration regimens we used. However, treatment with protegrin-1 caused a decrease in the ascites volume and in the number of cells in the ascites fluid.
CONCLUSIONS: Protegrin-1 retains its antitumor properties in vivo, but it may be presumed, that to effectively suppress tumor growth it requires a more frequent and prolonged administration compared with conventional antitumor antibiotics, of which we adopted the administration regimen for protegrin-1 in this study.
The Eukaryotic System in the third update of the “Eukaryotic Supergroups: Taxonomy/Biotechnology Interface” (2024)
Authors: Zmitrovich Ivan V. , Perelygin Vladimir V. , Zharikov Mikhail V.
Published: October 2024

Updates of the eukaryotic system (domain Eukarya) in the interface “Eukaryotic supergroups: Taxonomy/Biotechnology interface” are described. The nine-kingdom system Eukarya has the following form: subdomain Excavata (kingdoms Archezoa, Discoba), subdomain Obimoda (kingdoms Crumalia, Amoebozoa, Obazoa), subdomain Corticata (kingdoms Eocorticata, Haptista, Tsaralia, Plantae incl. Cryptista, Glaucophyta, Rhodophyta, Chloroplastida).
Standardization of color measurement in the medical photography in clinical practice
Authors: Savelyev Dmitry S. , Gorodkov Sergey Yu. , Goremykin Igor V.
Published: October 2024

BACKGROUND: For many medical specialties, photography is a routine element of work. Environmental factors equally and significantly influence the interpretation of colour light perception and the quality of photographic recording. Standardization of conditions in medical photography is necessary for subsequent quantitative assessment of the postoperative skin scar by analysing colour coordinates in the RGB system.
AIM: To determine the impact of illumination and shooting distance at colour coordinates when studying unaltered skin.
METHODS: Five volunteers without any skin diseases and no make-up on their facial skin were taken in the study. Skin type by the Fitzpatrick scale was II–III. Digital photography was made with a mobile device camera at distance 30 cm from the subject. For each participant, a series of digital photographs was taken at a distance of 20, 30, 40, 50, 60, 70 cm from the light source. Colour coordinates in the RGB system were measured in the digital graphics editor Adobe Photoshop CS6. Statistical analysis of the obtained data was carried out using Microsoft Office Excel 2019, Phyton 3.11.
RESULTS: The analysis of variance by the ANOVA method was used as a statistical analysis. In order to determine a statistically significant difference between the sets, Tukey HSD test was performed. A total of 1764 coordinates of three colours were subjected to statistical analysis (R=588, G=588, B=588). When constructing a heat map of the cross-correlation of the absolute values of each colour at each distance, taking into account the type of light source among themselves, the data had a strong direct correlation, regardless of the study area. When constructing a linear graph, coordinates of any of the zones were located on the same straight line.
CONCLUSION: Photography conditions were experimentally determined under which the color interpretation of light is constant. Results of the study should be taken into account during medical photography and subsequent color assessment of postoperative skin scars.
Dynamic Ruptures on Bending Fault: Insights from Numerical Simulations of Transient Stress Field
Authors: Liu Yuhang , Qian Feng , Zhang Haiming
Published: October 2024

We delve into the spontaneous rupture propagation on bending faults by numerical simulations based on the boundary integral equation method with unstructured meshes. To study the effect of fault geometry on dynamic rupture propagation, special attention is paid to the role of the dynamic stress field. The numerical results demonstrate that the bending angle is a key geometrical factor influencing the rupture propagation because it affects both the initial stress distribution and the dynamic stress field on the bending branch. The rupture propagation on the bending branch can be separated into two distinct stages: first, the propagation from the main branch to the bending branch, which largely depends on the dynamic stress field near the bend; and second, a subsequent propagation stage primarily influenced by the initial stress state on the bending branch, with the influence of the dynamic stress field decreasing rapidly with distance from the bend. Geometrical smoothing of the bend can be regarded as a modification of the bending angle, which may significantly alter the behavior of rupture propagation near the bend. In theory, if the bending angle ranges between −120° and 60°, there is a potential for rupture to propagate onto the bending branch through the bend.
Desafíos digitales en la práctica educativa: Una perspectiva del docente universitario
Authors: Mercado Hermenegildo Arturo
Published: October 2024

El presente estudio tuvo el propósito analizar los desafíos digitales en la práctica educativa desde una perspectiva del docente universitario, para tal fin se predispuso una investigación básica, de enfoque cualitativo, la cual tuvo como principal característica describir y explicar los hechos en mención, la muestra estuvo conformada por cinco docentes de nivel universitario, a los cuales se aplicó una guía de entrevista, los datos hallados fueron ingresados al programa de asistencia de textos Atlas Ti versión 9, donde se determinó las relaciones y coocurrencias de las subcategorías e indicadores pertenecientes a la categoría principal, los resultados evidenciaron que la tecnología digital es reconocida como útil y sirve a los docentes como puente de tránsito para lograr una conexión efectiva entre el conocimiento pedagógico y la generación de contenido, en este sentido se concluyó que las tecnologías digitales presentan una percepción positiva entre los docentes, y que de manera general estas tecnologías ya han sido adoptadas por la gran mayoría profesores, quienes la emplean en su quehacer académico diario, resaltando el benefició de entregar una inmediata retroalimentación, y una apertura a diferentes recursos educativos.
Percepción y Aceptación del B-Learning en Matemáticas para Ingeniería por Estudiantes: Un Enfoque Basado en TAM
Authors: Trejo Elia , Trejo Trejo Natalia
Published: October 2024

Este estudio explora la aceptación del b-learning en clases de matemáticas para estudiantes de ingeniería, utilizando el Modelo de Aceptación de la Tecnología (TAM) como marco teórico. Con una muestra de 153 estudiantes de una universidad tecnológica en México, se evaluaron constructos clave como la Utilidad Percibida (PU), la Facilidad de Uso Percibida (PEOU), la Influencia Social (SI), la Facilidad de Condiciones (FC), la Actitud hacia el Uso (AT) y la Intención de Uso (BI). Los resultados mostraron que el TAM es un predictor fiable de la aceptación del b-learning, con un índice de fiabilidad del instrumento de α = 0.9, lo que respalda su capacidad para identificar las variables que influyen en la adopción de esta modalidad educativa. El análisis reveló que la actitud hacia el b-learning es un determinante significativo de la intención de aprender matemáticas mediante este método, mientras que la influencia social, aunque relevante, no mostró una relación directa con la actitud hacia el aprendizaje. La facilidad de condiciones, incluyendo el acceso a internet y el apoyo docente, influyó positivamente en la percepción de utilidad y facilidad de uso del b-learning. Estos hallazgos subrayan la importancia de garantizar recursos tecnológicos adecuados y apoyo institucional para maximizar la eficacia del b-learning en la enseñanza de matemáticas. Se sugiere que futuras investigaciones exploren otras variables predictoras no consideradas en este estudio para un análisis más completo del TAM en contextos educativos.
Aplicación del proceso inmediato por flagrancia delictiva y vulneración del derecho de defensa en procesos por delito de robo agravado en el módulo básico de justicia de Alto de la Alianza, año 2019
Authors: Silva Napuri David Moises
Published: October 2024

El objetivo de la investigación fue analizar la aplicación del proceso inmediato por flagrancia delictiva frente a la vulneración del derecho de defensa en el debido proceso en el delito por robo agravado. El enfoque metodológico del estudio se basó en una investigación de tipo básica y de nivel descriptivo, considerando una muestra aplicada al personal jurisdiccional del Módulo Básico de Justicia de Alto de la Alianza en la ciudad de Tacna- Perú. De lo que se concluye que, con base a los argumentos previamente expuestos, el derecho de defensa que de manera expresa está reconocido en la Carta Magna del Perú, debe ser la premisa a la cual tienen que ajustarse todas las propuestas de corte legislativo relacionadas a la materia procesal penal, y en especial referencia al D. L. N° 1194, en concordancia al principio de jerarquía normativa.
Awareness, Attitudes, and Actions About Environmental Southern Appalachia
Authors: Thibeault Deborah , Jamie Clem , Cherry Donna
Published: October 2024

The Council on Social Work Education (CSWE) requires social work programs to integrate environmental justice into their programming. The purpose of this study is to determine if undergraduate and graduate social work students at a southern Appalachia university had an increase in awareness, a shift in attitudes, and a rise in action related to environmental justice since starting their program. This study also sought to identify where students felt environmental justice content would best fit in the social work curriculum. Participants completed a semi-structured focus group or an open-ended survey answering identical questions. Findings indicate the need for social work educators to provide students with clarification of environmental conservation and environmental justice; an understanding of the breadth of environmental justice across the micro, mezzo, and macro-levels; and the opportunity to have direct practice experiences and reduce feelings of being overwhelmed by the enormity of environmental justice. Participants endorsed the importance of environmental justice content in the curriculum.
“We Continue to Keep This Crisis in the Forefront”
Authors: Morris Kendall , Sprague Martinez Linda
Published: October 2024

Homicide has an effect on the community, as family members are left to grapple with the loss. The Women Survivors of Homicide Movement (WSOHM) is a grassroots effort aimed at empowering women of color to advocate for themselves as a homicide survivor. A systematic analysis was conducted to examine how power and privilege impact the groups’ approach to change-making. Mixed research methods were employed including key informant interviews and document review. Findings indicate WSOHM employs multiple strategies to achieve its goals, which are focused on bringing attention to unsolved murders in Boston and ensuring survivors and victims are given respect.
Resuscitating Equality
Authors: Rao Sandy
Published: October 2024

Within the evolving healthcare sector, the focus on health equity has led to interventions that, despite good intentions, often fall short. Recognized by a broad spectrum of healthcare professionals, including numerous social workers, as marginally better than inaction—colloquially referred to as “better than nothing,” these efforts risk overshadowing deeper structural and systemic issues. Consequently, they divert attention from the need for comprehensive solutions that genuinely address the roots of healthcare inequities. This manuscript delves into the nuanced interplay between health equity and equality through the methodology of critical analysis, drawing on the insights of critical social work and relevant theories of justice and power. While contemporary discussions increasingly restrict equality to uniform resource distribution, the core of social justice emphasizes equality’s deeper significance: recognizing the inherent worth of every individual, regardless of their background. The primary objective of this article is to advocate for the “resuscitation” of equality in healthcare, aligning it alongside health equity to ensure a comprehensive approach for individuals and families. A reductionist view of equality may cloud essential structural health determinants and compromise truly equitable care. The ramifications for social work are clear: a fervent advocacy for both equality and equity is indispensable. By embracing equity and equality in their most nuanced dimensions, we ensure that individuals, irrespective of their unique circumstances, receive care that is both just and tailored, elevating the benchmark in healthcare delivery.
A study to compare abdominal wall closure with interrupted vs. continuous sutures using polydioxanone in patients undergoing emergency exploratory laparotomy
Authors: Chauhan Pallavi , Gupta Jagdish , Bhardwaj Iti , Purohit Arjun , Vishwanath Atul , Pahal Divya
Published: October 2024

Background: Wound closure in emergency surgeries can be done either by interrupted or by continuous sutures with each having its own advantages and disadvantages. In this paper we shall try to compare the advantages and adverse effects of both these methods of wound closure.
Methods: A prospective comparative study involving 50 patients undergoing emergency laparotomies was conducted in Department of General Surgery, MMIMSR, Mullana from October 2022 to April 2024 (18 months). We compared two abdominal wall closure techniques using polydioxanone (PDS), patients, aged 18-75 years, were alternatively assigned to one of two groups: Group I received interrupted PDS sutures, while Group II received continuous PDS sutures. Postoperatively, pain and complications like infections were observed and analysed statistically.
Results: In our study, we compared abdominal wall closure techniques in emergency exploratory laparotomy patients. The mean age was similar in the two groups, 43.68 years vs 43.08 years respectively in group I and II. Wound infection occurred in 44% of patients in the interrupted suture group and 60% in the continuous suture group, with a significant difference favouring the interrupted method. The mean wound closure time (33.24 vs 18.32 minutes) and post operative pain were more in the interrupted suture group as compared to the continuous suture group.
Conclusions: The interrupted suturing method proved to be more effective in preventing complications, it did require more time to perform. However, after thoroughly reviewing the available data, no definitive evidence was found to support the superiority of either techniques.
Bromelain based enzymatic debridement in burn patients, its outcome, advantages and disadvantages
Authors: Shah Kesha C. , Srivastava Sunil N.
Published: October 2024

The aim of this study is to evaluate the outcomes, advantages, and disadvantages of bromelain-based enzymatic debridement in the management of burn wounds. Ours is a study in which we have selected 10 patients with deep dermal and full thickness burns admitted in SMS hospital, Jaipur. These patients underwent bromelain based enzymatic debridement applied as per the manufactures protocol. The patients were then observed for bromelain’s efficacy in debridement, wound healing, pain, duration of hospital stay, superadded bacteraemia and cosmetic outcome. Complete removal of burn eschar was observed in 9 out of 10 patients. Bromelain-based enzymatic debridement effectively removed necrotic tissue within a shorter timeframe compared to traditional methods. Patients treated with bromelain-based enzymatic debridement showed significantly faster wound healing, reduced infection rates, and lower pain levels during debridement. Improved cosmetic outcomes, such as reduced scarring and better skin texture, were also observed. Bromelain-based enzymatic debridement offers notable advantages in burn care, including expedited healing and enhanced patient comfort. Despite these benefits, the treatment's higher cost and the risk of allergic reactions are important considerations. The limited availability of bromelain-based products also poses a challenge. These factors suggest that while bromelain is a promising alternative, its use should be carefully evaluated based on individual patient needs and resource availability.
A rare case of acute colonic pseudo-obstruction leading to colonic perforation in a ventilated patient with cerebrovascular accident
Authors: Bandaru Srinivasa Swamy , Hamed Heba Abdelhafiz , Al Masalmeh Fadi Hassan
Published: October 2024

Acute pseudo-colonic obstruction in a patient with a functioning ileocecal valve can be risky, resulting in closed-loop obstruction, significant distention of the colon, and increased chances of colon perforation and ischemia if diagnosis and timely non-operative treatments are delayed. We report a case of acute pseudo-colonic obstruction with closed-loop obstruction, resulting in colon perforation in a ventilated patient with a cerebrovascular incident. The situation was promptly identified during the first assessment with a surgical consultation and imaging study, resulting in timely surgical intervention which included removing the diseased section of the right colon, creating an end ileostomy, and colostomy. The postoperative contrast study of the remaining colon and colonoscopy confirmed the pseudo-obstruction without finding any blockages in the distal colon and rectum. After the operation, the patient recovered well and was sent home. In conclusion, early clinical suspicion, diagnosis, and prompt management of a complicated ACPO result in a good patient outcome.
Squamous cell carcinoma of scrotum mimicking as non-healing ulcer: a case report
Authors: T. Varadheswari , Priya B. Sathya , C. Vivekanand
Published: October 2024

Scrotal cancer is an extremely rare malignancy, with squamous cell carcinoma (SCC) being the most common histological subtype. Despite its rarity, the condition presents significant diagnostic and therapeutic challenges, particularly in cases of delayed presentation and atypical features such as abscess formation within the tumor. A 51-year-old male with diabetes presented with a three-year history of a non-healing ulcer on the scrotum. Clinical examination revealed a distinct ulcer with everted margins, necrotic tissue, and inguinal lymphadenopathy. Radiological investigations, including pelvic MRI and inguinal ultrasonography, confirmed scrotal wall thickening and enlarged lymph nodes bilaterally. The patient underwent wide local excision of the scrotal ulcer, left orchidectomy, and ilioinguinal lymph node dissection. The patient recovered without complications. Histopathological examination confirmed infiltrating moderately differentiated squamous cell carcinoma, staged as T2N1M0. The findings underscored the complexity of diagnosis and the necessity for a multidisciplinary treatment approach. This case highlights the diagnostic difficulties associated with scrotal SCC, particularly in the context of delayed presentation. Early detection and comprehensive treatment strategies, including surgical excision and lymph node dissection, are critical for improving patient outcomes in this rare malignancy.
Asymptomatic presentation of diffuse large B-cell lymphoma as isolated submental lymphadenopathy
Authors: Sahoo Kalpit K. , Mani Kanika , Singla Rajveer , Arora Bhavinder K. , Mahipal  , Pal Naresh
Published: October 2024

Non-Hodgkin lymphoma (NHL) is the most common type of lymphoma, Diffuse larger B-cell lymphoma (DLBCL) being most common type of NHL. Patients usually present at later stage with b-symptoms, lymphadenopathy and organomegaly. Isolated asymptomatic lymphadenopathy is a rare presentation. A 50-year-old female presented with complaints of swelling in the sub mental region. It increased in size over 1month without any associated local or systemic symptoms. On examination a firm, non-tender, mobile swelling of around 2×1.5 cm was present at sub mental region. Abdominal examination revealed no organomegaly. Blood picture- HB- 9.7 gm/dl, TLC- 5200/mm3, neutrophils/ lymphocytes/ eosinophil/ basophils-43.4/44.4/5.1/0.4 and Platelets-2.41 lakhs/mm3. Ultrasonography study was suggestive of necrotic lymph node. Fine needle aspiration cytology demonstrated nonspecific reactive lymphadenitis. After routine pre-operative workup patient was taken up for lymph node excision in elective OR under general anesthesia and the excised lymph node was sent for biopsy. A histopathological diagnosis of diffuse large B-cell lymphoma was made and confirmed with Immunohistochemistry (IHC), (CD20-diffuse positive; CD3-focal positive; Cyclin D-negative; BCL6-positive; CD10- focally positive). Post-operative hospital stay was uneventful. Our case was atypical with asymptomatic presentation with isolated lymphadenopathy with suspicious lymphocytosis. Diagnosis was only possible by histopathology.
Is There a “Social Turn” in Analytic Philosophy?
Authors: Kosecki Artur
Published: October 2024

W moim przekonaniu historia rozwoju XX-wiecznej filozofii analitycznej charakteryzuje się trzema głównymi fazami: (a) zwrotem ku analizie, (b) zwrotem lingwistycznym i (c) zwrotem naturalistycznym. W niniejszym artykule rozważam, czy ostatnie zainteresowanie subdyscyplinami, takimi jak (1) epistemologia społeczna, (2) ontologia społeczna, czy metody „inżynierii pojęciowej” stosowane m.in. w ramach (3) filozofii języka, może świadczyć o tym, że obecną fazę rozwoju w filozofii analitycznej określić można mianem (d) zwrotu społecznego.
Cuidados paliativos na emergência: um novo chamado para a educação médica no Brasil
Authors: Pantoja Jessica Corrêa , Manso Maria Elisa Gonzalez
Published: October 2024

Este estudo investiga a implementação e eficácia dos cuidados paliativos na área de emergência médica. Através de uma análise bibliográfica, foram identificadas deficiências e percepções divergentes sobre essa abordagem na medicina de emergência. As principais lacunas se concentram em várias áreas, evidenciando limitações e interpretações divergentes, especialmente na visão dos profissionais de saúde. Além disso, destaca-se a falta de conscientização sobre os cuidados no fim da vida, especialmente entre médicos de emergência menos experientes. Esse panorama reforça a urgência de pesquisas que esclareçam a aplicação efetiva desses cuidados nos prontos-socorros brasileiros, onde essa prática ainda não está consolidada. Conclui-se que uma mudança de perspectiva, voltando o foco não apenas para “salvar vidas”, mas também para preservar a dignidade humana, é essencial para uma prestação de serviços de emergência mais humanizada.
Pragmatic Analysis in Speech Acts (bahasa ogan) used between Buyers and Wanita Bakul in Batu putih Village
Authors: Shofiyatul Huriyah  , Emilia Contessa  , Nurhasanah  , Henny Yulia
Published: October 2024

This research aims to discover the types of locutionary, illocutionary, and perlocutionary speech acts used by buyers and Wanita Bakul in Batuputih village. The method used in this research is qualitative with descriptive type. The data collection techniques used are recording techniques and listening and recording techniques. The data source in this research was obtained from buyers and Wanita Bakul in Batuputih village, who engaged in speech act interactions using oganese language in buying and selling transactions. The researchers used proficient free involved listening, recording, and Wanita Bakul note-taking techniques to collect the data required in this research. The results show that the speech acts used in buying and selling interactions in  Batuputih village, total number of speech acts was 84, locutionary act 9, illocutionary act 25 and perlocutionary act 29. The perlocutionary act is the most dominant speech act between buyers and Wanita Bakul interactions in Batuputih village.
The Strong Object Invariant
Authors: Malbaški Dušan , Kupusinac Aleksandar
Published: October 2024

The concept of an invariant is fundamental to object-oriented programming, because it provides information on the overall behaviour of the class and/or its objects. An invariant is a predicate, that is true in every state that is proclaimed as valid. A strong invariant is a predicate, that is true in every valid state and false in every invalid state. Basically, we can divide them into two categories: object invariants and class invariants. Object invariants describe the consistency of object, i.e. non-static fields. Analysis of invariants takes the most important place in object-oriented program verification and can be directed in two ways – as prescribed and as described. This paper considers both analyses which are based on the strongest dynamic postconditions of methods with the guard as the precondition, thus, determining all possible transitions and only them. In addition, since dynamic postcondition are logical functions of the initial-final states, our solution is based solely on the first-order predicate logic.
Application of Business Intelligence in the Brazilian Educational System
Authors: Moniz Junior Carlos Grimm , Cavalcante Tristão Sócrates Baptista , Vieira Adalena Kennedy , Vieira Raimundo Kennedy
Published: October 2024

In this article, we will discuss an application of Business Intelligence (BI) in analyzing data from the Brazilian educational system, specifically working with INEP (The Instituto Brasileiro de Geografia e Estatística — IBGE). This paper then discusses BI implementation's positive contributions and critical challenges in this domain. From the construction of an integrated BI system, our study aimed to offer a helpful tool that was easy to handle and touched on visuals such as dashboards, tables, and graphs to help us understand and manage the educational scenery throughout Brazil. The methodology used has been applied, with case studies and bibliographical research, based on data analysis techniques such as ETL (Extract, Transform, Load) and Data Warehousing. This is all leveraged to interactive dashboards, which should enable data-driven decisions and forge the way for advanced analytics like data mining.
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Authors: Cancell Cancel
Published: October 2024

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Apresentação da edição especial "Ampliando horizontes curriculares: reconstruindo a educação a partir da diferença"
Authors: Rodrigues Ana Cláudia da Silva , Costa Hugo Heleno Camilo , Honorato Rafael Ferreira de Souza
Published: October 2024

Apresentação da edição especial "Ampliando horizontes curriculares: reconstruindo a educação a partir da diferença"