bluuebunny/crossref_metadata_2025 huggingface dataset:
Contenidos de Ciencias Naturales en la Educación Primaria en el pasado reciente en Santiago del Estero: un análisis de normativas nacionales y provinciales
Authors: Ithuralde Raúl Esteban , Dumrauf Ana Gabriela
Published: July 2021

Resumen Desde la década de 1990, se han construido dos propuestas de contenidos curriculares para la Educación Primaria a nivel nacional en Argentina, una bajo la Ley Federal de Educación y otra de la Ley de Educación Nacional. Las provincias debían producir diseños curriculares acordes a estas normativas; sin embargo, no todas lo han hecho. En Santiago del Estero, el Diseño Curricular vigente para el nivel primario data de 1997. Existe a su vez un Diseño Curricular para la Educación Primaria de Jóvenes y Adultos, de 2004. En este trabajo analizamos crítica y comparativamente los contenidos del área de Ciencias Naturales de los cuatro documentos curriculares anteriores. Uno de los principales resultados es que dichos contenidos parecen permanecer casi inmutables, a pesar de los cambios en política educativa acaecidos en el período estudiado.
School transportation program as means to improve public education in a minor rural town in Northeastern Brazil
Authors: Nascimento Maria Victória Leal de Almeida , Andrade Mauricio Oliveira de
Published: July 2021

Abstract School transportation is crucial for access and permanence of students at school, in rural areas. The Brazilian government has funded school transport programs to support municipalities. This research aims to analyze if these programs are perceived as effective in small rural municipalities. Seeking this objective, a survey was carried out among students and teachers. Quantitative and qualitative analyses were conducted to test the results. In the quantitative analysis, it is observed that the majority of students are very pleased, being regularity/punctuality the best-evaluated criterion. Teachers believe punctuality has improved, school evasion has decreased, and school performance has increased. In a combined analysis, the evaluations carried out by teachers regarding school evasion and performance presents a significant relationship with regularity/punctuality assessed by students. In the qualitative analysis, a grouping of ideas highlighted comfort, safety, civility and punctuality as positive, while most-cited negative points were overcrowding, lack of organization and discomfort.
CORPOS, AFETOS E SEXUALIDADES: OS CÍRCULOS DE CULTURA COMO POSSIBILIDADE DE EDUCAÇÃO EMANCIPATÓRIA
Authors: Vieira Joana Barral Lopes , Silva Maria Cecília de Paula
Published: July 2021

O texto propõe, por meio do processo teórico e prático dos círculos de cultura, refletir sobre os temas geradores: corpos, afetos e sexualidades com o objetivo de questionar práticas pedagógicas conservadoras que nos direcionam a determinados padrões de comportamento e concepções sobre os temas mencionados. Os círculos de cultura foram desenvolvidos numa turma de educação de jovens e adultos – PROJEA – de uma escola situada num quilombo urbano de Salvador e, a partir dessa interação educacional apresentamos alguns dos resultados desta investigação propositiva, baseados em entrevistas e conversas que relatam modos e linguagens que ajudam a compreender os temas geradores no ambiente escolar. Isso nos permitiu apontar e potencializar uma (re)construção e afirmação de uma educação direcionada para uma formação mais humana, ao questionar os marcos hegemônicos da sociedade e possibilitar a formação de uma cidadania capaz de promover a emancipação.
A IMPORTÂNCIA DA COMPREENSÃO DA CATEGORIA TRABALHO PARA OS PROFISSIONAIS QUE ATUAM NA EDUCAÇÃO PROFISSIONAL
Authors: Garcia Júlio Cézar , Afonso Lúcia Helena Ríncon , Araújo Denise Silva
Published: July 2021

RETOMAR: Este artigo analisa importância da fundamentação teórica da categoria trabalho e suas contradições na sociedade capitalista para os profissionais que atuam na Educação Profissional Técnica de Nível Médio (EPTNM), mais especificamente com aqueles que desenvolvem suas atividades por meio da concepção do Ensino Médio Integrado (EMI ) O estudo partiu de uma pesquisa bibliográfica, e teve como objetivo a reflexão sobre os modos como categoria de trabalho se formação na sociedade capitalista e sua formação na formação do ser social. Em um primeiro momento, o estudo parte da análise do trabalho como atividade criativa ser humano como categoria central do processo de sua humanização, de modo a compreender como ocorre a transformação do ser biológico em ser social, pela mediação do trabalho com a natureza. O segundo momento tem por perceber como o modo de produção capitalista transforma a criatividade do trabalho em uma atividade de alienação por meio de um processo que envolve a relação entre as coisas, com predomínio do objeto sobre o sujeito. Um final do estudo tem-se uma reflexão final que só a partir da compreensão das contradições da categoria trabalho no sistema de produção capitalista, será possível ao profissional da EPTNM o entendimento dos objetivos e finalidades da concepção omnilateral da EMI na sua prática pedagógica, uma vez que o princípio educativo do EMI é desenvolvido por meio da categoria de trabalho.
Effect of the installation process on monopile lateral response
Authors: Bienen Britta , Fan Shengsheng , Schröder Maximilian , Randolph Mark F.
Published: July 2021

Most offshore wind turbines (OWTs) are supported by large-diameter monopiles, installed by impact driving. OWTs are dynamically sensitive structures, with a narrow design range for the eigenfrequency. Fatigue and serviceability limit states can be critical in design, so the foundation stiffness plays an important role. The pile installation process results in changes to the soil state, investigated here through large-deformation numerical analyses using the coupled Eulerian–Lagrangian approach with a hypoplastic constitutive relation to model the sand. The soil state is then mapped to a small-strain model to evaluate the effect of the installation process on the lateral response. Complementary physical modelling results from monopile installation and lateral load testing in a centrifuge provide confidence in the numerical model. Analyses for two different sands highlight commonalities and differences. Comparison with results featuring wished-in-place or jacked installation illustrates the effect of changes in the soil state on the lateral response. The results characterise the changes in soil state and allow quantification of their effects as impact driving tends to produce a stiffer lateral response, with the expected variations due to pile dimensions and sand relative density. Further research will provide insights into the role of pore fluid response during impact driving and investigate vibro-driven piles.
Representaciones sociales de Educación Religiosa en la media académica: una apuesta desde la virtualidad
Authors: Benjumea-Loaiza Cetty , Vélez-Vélez Jesús Andrés
Published: July 2021

Objetivo: Describir las representaciones sociales que tienen los educandos de media académica de una Institución Educativa privada de Montería de la clase de Educación Religiosa. Método: La investigación se enmarcó en el enfoque cualitativo y el diseño de la doble hermenéutica, con técnicas como la cartografía social-pedagógica y el grupo focal, que, por la contingencia generada por el COVID-19 fueron aplicadas desde un entorno virtual. Resultados: Se evidenciaron las representaciones sociales que tienen los jóvenes sobre la clase de educación religiosa caracterizadas por ser un espacio confesional, asociado a la catequesis, que se plantea desde un modelo tradicional-transmisionista, fundamentado en el método escolástico y enfocado en la repetición de contenidos centrados en el cristianismo-católico; que además evade las perspectivas propuestas por los educandos; generando un sentimiento de opresión en estos últimos. Discusión y Conclusiones: A partir de los resultados, se presentó la necesidad de plantear un nuevo modelo de Educación Religiosa que permita a los educandos cultivar su identidad religiosa y desde ahí, estar en capacidad de entrar en diálogo con su realidad inmediata y mediata en términos de lo religioso; para desarrollar la capacidad de tomar postura frente a las diversas problemáticas religiosas contemporáneas que se le presentan.
PENGARUH MEDIA PENDINGIN TERHADAP KARAKTERISTIK MEKANIK DAN STRUKTUR MIKRO PADA PELAT BAJA KARBON RENDAH
Authors: Wicaksono Iskandarsyah , Ismarwanti Sri , Setiawan Jan , Susetyo Ferry Budhi , Syamsuir Syamsuir
Published: July 2021

PENGARUH MEDIA PENDINGIN TERHADAP KARAKTERISTIK MEKANIK DAN STRUKTUR MIKRO PADA PELAT BAJA KARBON RENDAH. Bahan baja karbon rendah mempunyai keuletan yang tinggi dan mudah dibentuk, sehingga banyak digunakan dalam dunia industri dan konstruksi bangunan. Namun bahan ini mempunyai kekuatan mekanik dan kekerasan yang rendah sehingga mudah aus.  Sifat mekanik dapat ditingkatkan dengan perlakukan panas yaitu dengan pemanasan pada temperatur tertentu dan ditahan beberapa waktu, kemudian dilakukan pendinginan. Media pendingin akan berpengaruh terhadap hasil peningkatan sifat mekanik. Oleh karena itu dalam penelitian ini akan dilakukan pengamatan terhadap karakteristik mekanik dan struktur mikro baja karbon rendah setelah perlakuan panas dan pendinginan pada media pendingin yang bervariasi. Proses pemanasan dilakukan pada suhu 950 °C dengan waktu penahanan 15 menit. Kemudian sampel tersebut didinginkan dengan variasi pendingin (aquadest, minyak goreng, oli dan tungku pintu terbuka). Hasil pengamatan pada sampel pelat baja karbon rendah (0,0524%) menunjukkan bahwa sampel tanpa perlakuan panas mempunyai nilai tegangan tertinggi yaitu sebesar 375,75 MPa dan pendinginan pintu tungku terbuka mempunyai nilai tegangan terendah yaitu sebesar 200,53 MPa. Nilai regangan tertinggi terjadi pada pendinginan pintu terbuka sebesar 38,77% dan nilai regangan terendah yaitu sebesar 25,49% terjadi pada pendinginan minyak goreng. Pengamatan struktur mikro pada material sebelum dan sesudah dilakukan perlakuan panas menunjukkan adanya ferrit dan perlit.Kata kunci: Perlakuan panas, media pendingin, sifat mekanik, pelat baja karbon rendah.
KARAKTERISASI KANDUNGAN URANIUM DAN UNSUR JEJAK PELET SINTER UO2 UNTUK FORENSIK NUKLIR
Authors: Torowati Torowati , Ngatijo Ngatijo , Rahmiati Rahmiati , Mustika Deni , Yusnitha Erilia , Yulianto Tri , Setiawan Jan
Published: July 2021

KARAKTERISASI KANDUNGAN URANIUM DAN UNSUR JEJAK PELET SINTER UO2 UNTUK FORENSIK NUKLIR. Forensik nuklir merupakan salah satu alat yang penting didalam keamanan nuklir terkait dengan penegakan hukum. Hal ini dikarenakan  keberadaan bahan nuklir dan  radioaktif memiliki potensi bahaya baik dari segi keselamatan, kesehatan hingga ancaman dalam keamanan nuklir.  Didalam  forensik nuklir, sidik jari adalah karakteristik bahan nuklir dan radioaktif  untuk memberi  informasi asal-usul suatu bahan nuklir sehingga diharapkan mempunyai data-data dari bahan nuklir dan radioaktif. Data-data diperoleh dari hasil  karakterisasi berupa pengujian baik  pengujian secara fisika ataupun kimia. Pengujian secara fisika seperti pengamatan visual, dimensi, fasa sedangkan secara kimia antara lain penentuan unsur bahan nuklir, penentuan konsentrasi unsur–unsur  dalam bahan nuklir. Dalam makalah ini telah dilakukan pengujian kandungan uranium dan unsur jejak dalam bahan nuklir pelet uranium oksida (UO2) dengan tujuan untuk sidik jari dalam mendukung forensik nuklir yang ada di PTBBN, BATAN. Pengujian kandungan uranium dilakukan secara titrasi potensiometri sedangkan pengujian unsur jejak selain uranium dengan metode spektrofotometri serapan atom. Hasil rerata pengujian kandungan uranium dalam bahan nuklir  dan radioaktif tersebut diperoleh antara  87% sampai 88% hal ini menginformasikan bahwa bahan tersebut adalah bahan nuklir UO2.  Hasil pengujian kandungan unsur jejak selain uranium dalam pengujian ini berbeda pengayaan maka kandungan unsur jejaknya berbeda pula, sehingga dapat menginformasikan tentang tingkat pengayaan uranium yang dimiliki oleh pellet UO2 tersebut. Data-data tersebut dapat digunakan sebagai sidik jari dalam forensik nuklir sehingga dapat membantu penyidik dalam indentifikasi pada forensik nuklir apabila terjadi penyelewengan atau penyalahgunaan dari jenis bahan nuklir tersebut.Kata kunci: Uranium, pelet sinter, sidik jari, forensik nuklir.
PENGARUH DISTRIBUSI UKURAN SERBUK UO2 PENGAYAAN 2%–5% PADA DENSITAS PELET UO2
Authors: Mutiara Etty , Hutapea Odi Buana , Dewayatna Winter , Yulianto Tri
Published: July 2021

PENGARUH DISTRIBUSI UKURAN SERBUK UO2 PENGAYAAN 2%–5% PADA DENSITAS PELET UO2. Telah dilakukan studi fabrikasi pelet UO2 sinter menggunakan serbuk UO2 pengayaan 2%, 3%, 4% dan 5% untuk mendapatkan pelet UO2 dengan densitas yang memenuhi syarat sebagai bahan bakar PWR melalui proses kompaksi dan penyinteran. Sepanjang pengetahuan penulis, tidak ada publikasi penelitian tentang hubungan distribusi ukuran serbuk dengan densitas pelet UO2 pada rentang ukuran serbuk yang dipelajari pada penelitian ini. Untuk itu, melalui penelitian distribusi ukuran serbuk UO2 dan fraksi berat masing–masing rentang ukuran serbuk dan pengaruhnya terhadap densitas pelet UO2 akan diperoleh sifat mampu kompak dan mampu sinter masing–masing serbuk UO2 melalui pengukuran densitas pelet mentah dan pelet sinternya. Penelitian ini diawali dengan proses ayak serbuk UO2 umpan untuk mendapatkan data distribusi ukuran serbuk beserta fraksi beratnya, dilanjutkan dengan pengukuran densitas curah dan densitas ketuk. Masing–masing serbuk dengan distribusi ukuran dan fraksi beratnya langsung dikompaksi menjadi pelet mentah dengan variasi tekanan 2, 3 dan 4 ton/cm2, kemudian disinter pada temperatur 1700 °C selama 3 jam. Hasil pengayakan memberikan data bahwa serbuk UO2 pengayaan 2% dan 4% mempunyai distribusi ukuran serbuk monomodal sementara serbuk UO2 pengayaan 3% dan 5% dengan trimodal. Densitas pelet hasil kompaksi serbuk UO2 pengayaan 2%, 3%, 4% dan 5% pada tekanan kompaksi tertinggi berturut–turut sebesar 54,30%TD, 53,19%TD, 52,45%TD dan 52,33%TD. Densitas pelet sinter dari serbuk UO2 pengayaan 2% dan 5% masing–masing berada pada kisaran (91,13 – 91,70) %TD dan (75,33–77,57) %TD sementara pelet sinter pengayaan 3% dan 4% semuanya retak/pecah sehingga densitas tidak dapat diukur. Berdasarkan data densitas pelet disimpulkan bahwa serbuk UO2 pengayaan 2% dengan distribusi ukuran monomodal yang didominasi serbuk berukuran halus yang lebih kecil 75 µm mempunyai mampu kompak dan mampu sinter yang lebih baik dari serbuk lainnya. Penelitian ini perlu dilanjutkan untuk mengetahui lebih jauh berkaitan pemilihan distribusi ukuran serbuk UO2 umpan dengan fraksi beratnya sebagai umpan proses kompaksi dalam upaya mendapatkan pelet UO2 sinter dengan densitas lebih tinggi yang memenuhi persyaratan sebagai bahan bakar PLTN tipe PWR.Kata kunci: Pelet UO2 PWR, distribusi ukuran serbuk, densitas, kompaksi, penyinteran.
The impact of the COVID-19 regulations on rent obligations
Authors: Viljoen Sue-Mari
Published: July 2021

SUMMARY The COVID-19 pandemic has led to the introduction of a range of regulatory measures, which has had a detrimental impact on the rights of South Africans, in general, and specifically the ability of commercial lessees to trade. A large number of commercial lessees were forced to close their businesses for lengthy periods of time, which effectively meant that they were deprived of the use and enjoyment of their leased premises. It is unclear whether such lessees, who have been either partly or absolutely deprived of the use of their premises, should continue to make rental payments. The common law is explored to cast light on this issue, taking account of the use of force majeure clauses and the operation of the lockdown measures as a form of vis maior. The common law position regarding vis maior and its impact on rent obligations is further considered with reference to regulatory measures that were specifically introduced to assist parties that were negatively affected due to the lockdown measures in the commercial rental sector.
Prediction of water reuse potential in South African water management areas: A linear regression and Bayesian network approach
Authors: Mamane S , Abimbade A , Ilemobade A
Published: July 2021

Increasing scarcity of freshwater resources and growing environmental awareness have given rise to the use of treated wastewater as an additional source of water supply. However, the amount of wastewater that can be reclaimed for reuse is subject to many factors, ranging from technical to socio-economic, environmental and institutional. This paper presents two prediction models for wastewater reuse potential in South Africa. The proposed models, namely a Bayesian Network model and a Linear Regression model, were used as a platform for integrated analysis of features such as water use, and socio-demographic and environmental factors. A dataset from the sub-regions of the 19 water management areas in South Africa was employed as input into the models in order to analyse these features and to consequently predict water reuse potential.
A critical review of the Water Sorptivity Index (WSI) parameter for potential durability assessment: Can WSI be considered in isolation of porosity?
Authors: Moore A J , Bakera A T , Alexander M G
Published: July 2021

The Durability Index (DI) approach used in South Africa is linked to transport-related properties of the cover layer of concrete. This performance-based approach utilises three different durability-related parameters, namely Water Sorptivity Index (WSI), Oxygen Permeability Index (OPI) and Chloride Conductivity Index (CCI). In the water sorptivity test, both the sorptivity and the water-penetrable porosity of the concrete are measured. Until now, porosity has not been considered directly as a separate durability-related parameter. This paper analyses these implications and suggests that porosity should be adopted as a further index. The paper concludes that sorptivity cannot be interpreted without also reflecting the porosity value since these two parameters are inter-related.
Matemática aplicada y prácticas sociales: escenarios de debate alrededor del currículo de matemáticas
Authors: Flórez Rojano Iván Darío , Céspedes Guevara Nelly Yolanda , Zamora Coronado Hugo Edver
Published: July 2021

En este artículo de reflexión se recogen algunos retos y tensiones actuales de la Educación Matemática, dentro de las cuales se incluyen: la pertinencia de los currículos en los diferentes ciclos educativos, las demandas de la sociedad hacia la escolarización de las matemáticas, los enfoques pedagógicos y didácticos para el área, y la formación de docentes para la educación matemática. Estos cuatro aspectos se interconectan y se debaten desde la problematización del currículo de Matemática, como resultado se destaca una permanencia de la tendencia a nivel Latinoamérica a desarrollar currículos centrados en los conocimientos, la ejercitación de procedimientos y el pensamiento algorítmico. Se discute como alternativa opuesta a esta corriente, una perspectiva centrada en los procesos matemáticos de la modelación y la resolución de problemas, desde un enfoque contextualizado coherente con las prácticas sociales y culturales.  Esta mirada ofrece una contribución al ámbito académico, recapitulando nuevas conceptualizaciones, reformulaciones y propuestas metodológicas en el desarrollo del debate sobre la estructuración del currículo de matemáticas en los diferentes ciclos educativos.
O ENFOQUE CTS NA EDUCAÇÃO PROFISSIONAL E TECNOLÓGICA: UMA REVISÃO DO CAMPO ENTRE OS ANOS DE 1995 A 2020
Authors: Braga da Silva Elison Victor , Albino Nunes Albino Oliveira Nunes , Josivânia Dantas Josivânia Marisa Dantas
Published: July 2021

Em um mundo onde cada vez mais se prioriza o desenvolvimento da Ciência e Tecnologia (C&T) com pouca, ou quase nenhuma, relevância as suas implicações sociais, uma educação que busque a formação ampla dos sujeitos é um dos grandes desafios contemporâneos. Nessa perspectiva, o enfoque Ciência-Tecnologia-Sociedade (CTS) aliado à Educação Profissional e Tecnológica (EPT) podem figurar como viáveis para a promoção de uma educação científico-tecnológica pautada na criticidade e cidadania. Assim, esse artigo buscou realizar um estudo exploratório, do tipo estado da arte, a respeito da produção acadêmica que versa sobre CTS em revistas que tratam da EPT, Educação Tecnológica e Trabalho e Educação. Para isso, foi realizado um levantamento de artigos científicos nas bases de dados de 12 periódicos brasileiros que debatem a educação profissional e tecnológica, compreendendo o período entre os anos de 1995 a 2020. Os dados foram avaliados por meio da análise de conteúdo de Bardin (2002) e da estatística textual apoiada no Iramuteq. Os resultados demonstram que há uma predominância de trabalhos teóricos voltados ao ensino superior, divididos em quatro temáticas, sendo os autores mais citados Bazzo, Auler, Santos e Mortimer e Von Linsingen.
Conciencia metacognitiva en docentes de diferentes áreas de dominio en una universidad pública de Manizales (Colombia)
Authors: Dussan Lubert Carmen , Ruiz-Ortega Francisco Javier , Montoya Londoño Diana Marcela
Published: July 2021

El presente artículo de investigación tuvo como objetivo describir la conciencia metacognitiva de una muestra de docentes universitarios y establecer posibles diferencias por género y áreas de dominio. Se realizó un estudio descriptivo correlacional de carácter transversal con una muestra de 323 docentes de una universidad pública colombiana, que fueron evaluados con el inventario de conciencia metacognitiva para el docente (metacognitive awareness inventory for teachers, mait). Para ello se analizaron los componentes del conocimiento (declarativo, procesual, y condicional) y de la regulación (planificación, monitoreo y evaluación); los análisis estadísticos se adelantaron mediante el software estadístico xlstat 2014. Entre los resultados se encontró, para todos los ítems, correlación significativa. Al comparar las medianas por sexo, se evidenciaron diferencias para el caso de la regulación metacognitiva, con un mejor desempeño para las mujeres. No se encontraron diferencias por área de dominio, salvo para el caso de los docentes del área de ciencias de la educación. Se recomienda en próximos estudios considerar variables como la edad, el nivel de formación y los años de experiencia docente, dado que podrían ser variables de interés en el análisis del constructo.
Seçilmiş Entegrasyon Örgütlerinde Satın Alma Gücü Paritesi Teorisinin Geçerliliğinin Fourier Fonksiyonu Kullanarak Fraksiyonel Birim Kökleriyle Test Edilmesi
Authors: ERGÜN TATAR Havanur
Published: July 2021

Satın alma gücü paritesinin özel terminolojisi, I. Dünya Savaşı’ndan sonraki yıllarda, başlıca sanayileşmiş ülkeler arasında, uluslararası politika tartışmalarının merkezine oturmuştur. Bu çalışmada, Fourier fonksiyonları kullanılarak, fraksiyonel birim kök incelemesi yapılmıştır. Teorem 1960-2019 dönemi için NAFTA, MERCOSUR, AB15 ve seçilmiş OECD ülkeleri gibi farklı entegrasyon örgütleri kapsamında ele alınmıştır. Çalışmada, Kesirli Frekanslı Fourier ADF ve geleneksel ADF testleri yapılmıştır. Trigonometrik terimleri anlamsız olan ülkelerde geleneksel ADF sonuçları hesaplanmıştır. Analiz sonuçlarına göre, MERCOSUR hariç diğer entegrasyon şekillerinde satın alma gücü paritesi hipotezinin geçerli olduğu tespit edilmiştir.
An Ode to Binomials: Linnaeus's Taxonomy
Authors: Troutman K. Roger
Published: July 2021

An Ode to Binomials: Linnaeus's Taxonomy is a prosetry (an integrated work of poetry and prose) with lyrical qualities on the history, description, value, and procedures of Linnaeus's binomial  nomenclature.
A revised circumscription and clarification of the taxonomic position of Miconia tetrazygioides (Melastomataceae: miconieae): an endemic to the Massif de la Hotte, Haiti
Authors: Judd Walter S. , Majure Lucas C.
Published: July 2021

An investigation of the type material of Miconia tetrazygioides indicates that this species, only known from the type gathering (Ekman H10684), is conspecific with the recently described M. cineana. The two differ vegetatively in no significant characters, showing only a slight difference in the range in variation of leaf width, shape, apex, and margin. The species has been poorly collected, with only a total of five gatherings known, and flowers have yet to be collected; it is endemic to the Massif de la Hotte, of Haiti, occurring in rak bwa from 930 to 1300 m. DNA nucleotide sequences indicate that the species is related to those of the M. decorticans clade, which have moderate-sized to large flowers with more or less constricted hypanthia/calyces. Traditionally, most species of the M. decorticans clade were included within Tetrazygia or Pachyanthus. In contrast the reproductive parts, e.g., calyx, hypanthium, ovary, and fruit, of M. tetrazygioides (incl. M. cineana) are quite small, and its hypanthia/calyces are not constricted, which is why it was originally circumscribed under Miconia rather than the other two mentioned genera.
Rediscovery, new county records, and distribution notes for Chloris texensis (Poaceae) in Texas, U.S.A.
Authors: Wipff Joseph K. , Rosen David J. , Singhurst J.R.
Published: July 2021

Rediscovery of the Texas endemic Chloris texensis in Brazoria County, and new records for Ft. Bend, Matagorda, and Waller counties are documented. Collection records outside the narrow Upper Coastal Prairie distribution are also reviewed.
IMU Garment test: Protocol-1 v1
Authors: Chakraborty Soma
Published: July 2021

Testing the first prototype version of Kymira IMU garment i.To check the reliability of measurement by the IMU-sensor network embedded in Kymira IMU-garment ii.To test the KYMIRA chain-IMU modules from imu-preprocessing to imu-jointangle
Flow Cytometry ECS Surface Antigens v1
Authors: Betts Michael , Golden Gregory
Published: July 2021

High-parameter flow cytometry enables identification and characterization of a wide range of cell populations within a biological sample. Analysis of cell-surface epitopes using a collection of fluorophore-labeled antibodies, or extracellular stain (ECS), sufficiently labels population-identifying markers for further analysis with flow cytometry. Importantly, ECS can be applied to cryo-preserved cell suspensions recovered in tissue culture media, enabling samples to be conveniently analyzed after collection and proper cryopreservation. However, consistent cryopreserved sample recovery and ECS procedures are critical to data comparison across multiple experiments. Herein, we describe a standardized protocol for cryopreserved sample recovery and ECS procedures for cell-surface epitope labeling.
HPAP Processing Protocol v1
Authors: Betts Michael , Golden Gregory
Published: July 2021

Early in life, a combination of environmental insults and genetic pre-disposition results in an autoimmune reaction to pancreatic b-cells in the Islets of Langerhans, leading to b-cell destruction and a lack of insulin production. Over time, near-complete loss of insulin production leads to Type I Diabetes (T1D), an often-deadly disease that requires lifetime treatment. Many studies have focused on immune system components that drive T1D autoimmunity, particularly the immune cells that infiltrate the islets of Langerhans. However, acute insulitis is extremely difficult to detect, as T1D pancreas samples are rare and individual islet destruction is thought to happen rapidly and sporadically. Therefore, studies have focused on autoimmune imprints within memory T and B cells that may develop after insulitis. Memory T and B cells that target b-cells would likely reside in lymph nodes that drain the pancreas and are potentially detectable in circulation or in the spleen. The Human Pancreas Analysis Program has organized a protocol to isolate single immune cells from the blood, spleen, and peri-pancreatic lymph nodes (PLNs) from healthy, pre-diabetic, and T1D patients. Herein, we describe the standardized protocol of single cell isolation from blood, spleen, PLNs, mesenteric LNs, and superior mesenteric LNs.
High-molecular weight DNA extraction from challenging fungi using CTAB for lysis and precipitation v3
Authors: Jones Ashley , Schwessinger Benjamin
Published: July 2021

Extracting pure high-molecular weight DNA from fungi is often difficult due to the presence of polysaccharides and potentially other compounds, which biochemically mimic DNA or interfere with the DNA extraction process. Such compounds can co-elute with DNA in many extraction methods, making them difficult to remove from solution. Some contaminants may even be undetected by spectrophotometers or fluorometric devices, however they still substantially interferes with long-read DNA sequencing. In attempt to resolve these challenges, a protocol is presented that utilses cetrimonium bromide (CTAB) for lysis and precipitation. CTAB in the lysis solution acts as a cationic detergent to dissolve cell membranes and remove polysaccharides by the formation of CTAB-polysaccharide complexes that co-precipitate during chloroform phase separation. Using CTAB for DNA precipitation under low salt, low ionic conditions, CTAB-DNA complexes form while leaving polysaccharides in solution, resulting in significantly less co-precipitation than alcohol methods. The presented protocol also includes some updates to current strategies and incorporates two different options for DNA clean-up and size selection. Using this protocol, we have been successfully sequencing various fungi with a MinION (Oxford Nanopore Technologies). For wheat stripe rust Puccinia striiformis and wheat leaf rust Puccinia triticina, we performed the presented method followed by size selection with an automated gel purification system, with sequencing yielding 5.88 Gbp with an N50 of 34.79 kb and 4.88 Gbp with an N50 of 27.61 kb, respectively for the two fungi. These fungi are notorious for being recalcitrant and these results have been positive improvements on many other methods. To increase sequencing output for these samples, more work is needed to identify and remove the elusive contaminants. Other fungi, such as sorghum rot fungus Macrophomina phaseolina, we chose an alternative size selection method, precipitation with a polymer and salt solution. This sequencing yielded 13.71 Gbp with an N50 of 21.75 kb, while another strain yielded 9.72 Gbp with an N50 of 43.50 kb.
Viral Tagging and Grow: a scalable approach to capture and characterize infectious virus-host pairs v1
Authors: Bin Jang Ho , Chittick Lauren , Li . Fen , M M Sanderson Courtney
Published: July 2021

Viral tagging (‘VT’), as shown in the Fig. 1A and 1B, entails fluorescently labeling the DNA of wild virus particles (upper, A), mixing these with a target, cultivable host bacterium (lower, A), and assessing the population of cells via flow cytometry for a fluorescent shift attributed to ‘viral tagging’ (VT) (B). VT was developed with culturable marine isolates (1, 2) and more recently applied to deduce virus-host pairing with unknown hosts in the human gut (3). However, all virus-host pairs detected (via phage adsorption) do not necessarily represent a productive lytic infection due to reversible and/or non-specific binding of lysogeny (phage genome replicates with the host genome). Additionally, post-adsorption cellular defenses could all prevent successful infection. To capture and characterize infectious, individual virus-host pairs, we developed ‘Viral Tag and Grow (VT+Grow)’ by incorporating a plate-based ‘Grow’ step (C) to VT (A and B).
Transit Zones, Locales, and Locations: How Digital Annotations Affect Communication in Public Places
Authors: Lettkemann Eric , Schulz-Schaeffer Ingo
Published: July 2021

The article presents an analytical concept, the Constitution of Accessibility through Meaning of Public Places (CAMPP) model. The CAMPP model distinguishes different manifestations of public places according to how they facilitate and restrict communication between urbanites. It describes public places along two analytical dimensions: their degree of perceived accessibility and the elaboration of knowledge necessary to participate in place-related activities. Three patterns of communicative interaction result from these dimensions: civil inattention, small talk, and sociability. We employ the CAMPP model as an analytical tool to investigate how digital annotations affect communicative patterns and perceptions of accessibility of public places. Based on empirical observations and interviews with users of smartphone apps that provide digital annotations, such as Foursquare City Guide, we observe that digital annotations tend to reflect and reinforce existing patterns of communication and rarely evoke changes in the perceived accessibility of public places.
What Constitutes a Local Public Sphere? Building a Monitoring Framework for Comparative Analysis
Authors: Fischer Renate , Keinert Alexa , Jarren Otfried , Klinger Ulrike
Published: July 2021

Despite the research tradition in analyzing public communication, local public spheres have been rather neglected by communication science, although they are crucial for social cohesion and democracy. Existing empirical studies about local public spheres are mostly case studies which implicitly assume that cities are alike. Based on a participatory-liberal understanding of democracy, we develop a theoretical framework, from which we derive a monitor covering structural, social, and spatial aspects of local communication to empirically compare local public spheres along four dimensions: (1) information, (2) participation, (3) inclusion, and (4) diversity. In a pilot study, we then apply our monitor to four German cities that are comparable in size and regional function (‘regiopolises’). The monitoring framework is built on local statistical data, some of which was provided by the cities, while some came from our own research. We show that the social structures and the normative assessment of the quality of local public spheres can vary among similar cities and between the four dimensions. We hope the innovative monitor prototype enables scholars and local actors to compare local public spheres across spaces, places, and time, and to investigate the impact of social change and digitalization on local public spheres.
Metabolic status, ultrasound changes of liver and vascular artery wall in men with abdominal obesity
Authors: Serdyukov D Yu , Gordienko A V , Zhirkov I I , Fedorova A S , Sokolov D A , Dyidyishko V T , Apalko S V , Scherbak S G
Published: July 2021

The prevalence of metabolic, vascular and hepatic disorders in young and middle-aged men suffering from abdominal obesity was estimated. It was found that in men suffering from initial obesity, compared with patients with normal diet, a high frequency of arterial hypertension (67 and 37%, χ2=41,5; p
Prevalence and features of polymorbidity of servicemen of different age groups according to the results of their medical examination to determine the category of fitness for military service
Authors: Voronin S V
Published: July 2021

The prevalence of polymorbidity and its features in military personnel of various age groups was studied. It has been established that polymorbidity during medical examinations of military personnel occurs in all age groups, increasing with age. For each age group of military personnel, its characteristic polymorbidity models are formed. So, in the age group up to 35 years, polymorbidity occurs in one third of the examined and is characterized by both functional disorders of the circulatory system and digestive organs, and the presence of organic pathology of the circulatory system (congenital malformations (developmental defects), mitral and other valve prolapse), digestive organs (chronic gastritis, gastroesophageal reflux disease) and the musculoskeletal system (flat feet, dorsopathies). At the age of 35 to 45 years, polymorbidity occurs in93,5% of those examined and is characterized by the presence of a cardiac and gastroenteric component in all polymorbidity models, in 80% of the models - the musculoskeletal component, in 60% - the metabolic component, in 40% - the endocrine component. Polymorbidity occurs in 97,5% of those examined over the age of 46 years and is characterized by the presence in all models of polymorbidity of the cardiac, cerebrovascular and musculoskeletal component, in 75% of the models - the metabolic component, in 50% - the endocrine component.
Experimental modeling of deforming osteoarthrosis of the knee in small rodents
Authors: Sokolova M O , Komarov A V , Kokorina A A , Shakun D A
Published: July 2021

The results of experimental work on modeling deforming osteoarthrosis of the knee joint in small rodents are presented. The pathogenetic basis of this process is a violation of the blood supply and the distribution of physiological load in the joint. Variants of rigid and non-rigid limb immobilization systems are proposed. The results of morphofunctional assessment are given, indicating a violation of the anatomical and histological structure of the metaphysis, pineal gland and metaepiphysis of the femur and tibia. The leading role in the development of deforming osteoarthritis of the knee belongs to the violation of the physiological distribution of the dynamic load on the joint, and the most vulnerable to the progression of this process are subchondral structures. Violation of regional blood circulation of the lower limb of the animal was achieved by crossing the femoral artery. Limb immobilization was carried out for 4 weeks. The results were evaluated by the severity of changes in the morphological structure of the operated joints and radiographic examination. The high mitotic activity of cellular elements, revealed as a result of histological examination in all groups of animals, indicates the rapid formation of vascular collaterals in rats. It was noted that with non-rigid immobilization of the joint with a plaster cast, the cartilaginous tissue of the articular surface becomes thinner, while the subchondral structures change slightly. With severe immobilization of the joint in the external fixation apparatus, significant changes are revealed precisely in the structure of subchondral structures. In animals subjected to a two-week rehabilitation period after dismantling the external fixation apparatus, a further progression of the pathological process was noted. Thus, with severe immobilization of the limb in the experiment, it was possible to obtain a stable joint deformation corresponding to the 3rd clinical and radiological stage of osteoarthritis.
The study of biodegradation of thermo-polimerized collagen gels
Authors: Smirnova V F , Gavrilyuk I O , Aleksandrova O I , Vasiliev A S , Mashel T V , Churashov S V , Chernysh V F , Blinova M I , Kulikov A N
Published: July 2021

The possibility of biodegradation of thermopolymerizable collagen gel (without cultured stem cells) in various concentrations is considered. In an experiment, an unpolymerized collagen gel was injected into the conjunctival cavity during preliminary blepharography. A polymerized collagen gel was obtained by incubating a thermopolymerizing collagen gel at37°C for 30 min until complete polymerization, then the gel was placed on the ocular surface and fixed by blepharography. Collagen gel biodegradation was evaluated at the 1st and 4th hours after surgery. Evaluation criteria: visually by the change in the volume of the plugged conjunctival cavity, leakage of fluid through the intershoot spaces, and also 12 hours after the operation according to the volume of collagen gel remaining in the conjunctival cavity (after removal of blepharoraphic sutures). It was revealed that biodegradation of thermally polarizable collagen gel is carried out by the mechanism of its dehydration regardless of the state of the implanted gel (unpolymerized/polymerized). The volume of the dehydrated collagen framework is directly proportional to its concentration. To increase the biodegradation time of a thermopolarizable collagen gel, it is advisable to develop a method for retaining fluid inside the collagen frame.
Activators hemorrhagic fevers and them epidemiology
Authors: Moskalev A V , Gumilevskiy B Yu , Astapenko P V , Apchel V Ya , Solov’yev A I
Published: July 2021

The current epidemiological characteristics of pathogens of especially dangerous infections abroad and in the Russian Federation are given. The biology of the causative agents of hemorrhagic fevers, as well as their species and subspecies, is especially reflected. The biology and epidemiology of the most common causative agents of hemorrhagic fevers are described in more detail: Dengue, Ebola, Marburg, Lassa, Machupo (Bolivian), Hanin (Argentinean) fever, Rift Valley fever, Omsk, Congo-Crimean, and renal fever their intermediate hosts - mosquitoes and ticks, the options for getting these pathogens into the human body. The most severe and widespread human infections, caused by alpha viruses and flaviviruses, the most severe epidemics in various countries are characterized. The modern systematics and immunopathogenesis of the causative agents of these infections, the features of virological and immunological diagnostics using both classical and modern techniques are presented. The new pathogens of hemorrhagic fevers, which have been fatal in recent years, are described. Given the current tense situation associated with the causative agents of hemorrhagic fevers, measures are proposed that are aimed at preventing the entry of pathogens into the Russian Federation, as well as the algorithm of actions of employees of medical institutions when patients with suspected infection associated with causative agents of especially dangerous infections are admitted.
Vesiculation red blood cells. Its role in donor erythrocytes components
Authors: Vaschenko V I , Vilyaninov V N , Skripaj L A , Sorokoletova E F
Published: July 2021

The formation of microvesicles by blood cells: monocytes, platelets, granulocytes, erythrocytes and endothelial cells is the most important feature of intercellular interactions. Red blood cells form microvesicles to remove damaged cell components, such as oxidized hemoglobin and damaged membrane components, and thus extend their functioning. Two hypotheses have been put forward for the formation of microvesicles: programmed cell death (eryptosis) and clustering of the band 3 protein as a result of disruption of intercellular interactions. In the process of eryptosis, damage to hemoglobin and a change in the pathways of phosphorylation of membrane proteins, primarily protein of strip 3, weaken the strong bonds between the lipid bilayer and the cytoskeleton, which is accompanied by the transformation of the membrane, the formation of protrusions and their transformation into microvesicles. It was found that the formation of microvesicles by red blood cells is impaired in patients suffering from various pathologies of red blood cells: sickle cell anemia, glucose-6-dehydrogenase deficiency, spherocytosis, and malaria. Studies of the last decade show that a violation of the interaction between the membrane and the cytoskeleton is probably the main mechanism, since it is confirmed by data obtained in the study of structural changes in red blood cells of donor hemocomponents stored in a blood bank. Currently, studies on the effect of microvesicles on the safety of erythrocyte-containing blood components have become widespread. A discussion was resumed on the relationship between the number of accumulated microvesicles in blood components and the effectiveness of donor components for patients during transfusion, depending on the shelf life of the components. Detailed data on proteomic, lipidomic and immunogenic comparisons of microvesicles obtained from various sources are convincing in the identification of trigger stimuli causing the generation of microvesicles. Elucidation of the contribution of microvesicles obtained from red blood cells to inflammation, thrombosis, and autoimmune reactions confirms the need to further study the mechanisms and consequences of the generation of microvesicles by red blood cells of donor components used for transfusion medicine.
Peter the Great is the founder of the military medical education in Saint Petersburg
Authors: Milasheva N V , Samoilov V O
Published: July 2021

The study is dedicated to the founding date of medical (medical and surgical) schools at the General hospitals of St. Petersburg, which are the historical foundation of the Medical and Surgical (Military Medical) Academy. Archival documents from the funds of the Russian State Archive of the Navy, as well as published sources prove and confirm that Peter the Great is the founder of medical (medical and surgical) schools at the General hospitals of St. Petersburg. According to the ingenious converter of Russia, the establishment of medical schools in the military and naval capital of the Russian Empire was part of state reform plans, it was extremely necessary and mandatory for the development of domestic medicine. A historical review of Russian military legislation of the era of Peter the Great is presented, where issues of medicine are touched. Particular attention is paid to archival documents. The reports (programs) of the first archivist and president of the Medical Chancellery and the entire medical service of Russia, Robert Erskine, and his successor, archivist Ivan Lavrentievich Blumentrost, to the president of the Admiralty Board, General Admiral Count F.M., were examined and analyzed in detail. Apraksin on bringing the medical unit in the fleet in proper condition. In the report I.L. Blumentrosta dated December 3, 1719 explicitly said about the already established medical school at the Admiralty Hospital of St. Petersburg and about the conduct of training sessions in it. The submitted documents developed a plan for the organization of marine hospitals, calculated the staff of medical personnel in the hospital and navy, reflected the plan for training medical students and preparing doctors, proposed solutions to other issues of organizing a medical service. The «Regulations on hospitals and on the positions of commissioners, doctors, clerks and others identified by them» of 1722, compiled on the basis of the programs of R. Erskine and I. L. Blumentrosta. This Regulation was the Russian hospital charter until the approval of the new law - the «General Regulation on Hospitals» (1735), which included 40 paragraphs of the Regulation 1722.
Diagnostic significance of otorhinolaryngological and ophthalmic pathology in patients suffering from neurosyphilis infected with human immunodeficiency virus
Authors: Orlova I. A. , Telichko S. I. , Smirnova I. A. , Dudko V. Y. , Piryatinskaya A. B , Telichko I. N.
Published: July 2021

It was found that eye damage can be considered as a marker of neurosyphilis. Similar data regarding the diagnostic value of damage to the ears, nose and throat in patients with syphilis infected with human immunodeficiency virus are single. It was revealed that neurological symptoms in patients suffering from neurosyphilis are equally common in patients infected with human immunodeficiency virus and those not infected with this virus (42,2 and 44,7%, respectively). In other cases, the disease is asymptomatic. At the same time, specific damage to the eyes, ears, nose and throat is diagnosed in 57,8% of patients with neurosyphilis infected with the human immunodeficiency virus. The structure of the specific damage to the ears, nose and throat in patients suffering from neurosyphilis infected with the human immunodeficiency virus is presented in 30,7% of cases with specific angina, in 15,3% - decrease in bone conduction, in 7,6% - hearing loss and in 46, 10% - other unspecified symptoms. The structure of the damage to the organ of vision was papillitis - 38,4%, anterior uveitis and panuveitis - 23,1 and 15,3%, respectively. Neuroveitis, retinal angiopathy, and peripheral chorioretinal dystrophy were equally common in 7,6%. For patients suffering from neurosyphilis infected with the human immunodeficiency virus, a combination of damage to the eyes, ears, nose and throat is characteristic. In particular, in one patient a combination of specific tonsillitis with papillitis was noted, in the other with uveitis. Thus, specific damage to the eyes, ears, nose and throat is characteristic of patients suffering from neurosyphilis infected with the human immunodeficiency virus.