bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploration of Metacognitive Skills And Student Critical Thinking Through Discovery Learning Method and Cognitive Style
Authors: PRAMUSİNTA Yulia , SETYOSARI Punaji , WIDIATI Utami , KUSWANDI Dedi
Published: December 2019

This research examines the effect of discovery learning method using various cognitive styles on metacognitive and critical thinking skills of pre-service elementary school teachers. Quasi-experimental pretest-post tests non-equivalent control group design is selected to be the method of this research. The subjects of this research are 144 pre-service teachers. The instrument used to measure the subjects’ metacognitive skill is Metacognitive Awareness Inventory (MAI) questionnaires as adapted from Schraw & Dennison (1994), whereas modified essays of Ennis (1985) are to evaluate critical thinking skills. Embedded Figure Test Group (GEFT) as adapted from Witkin, et al (1977), is utilized to measure the cognitive styles of the research subjects. The data is then analyzed through two-way MANOVA technique. The findings of the research conclude that: (1) there are significant differences in metacognitive and critical thinking skills between groups which learned using discovery learning method and discussion method; (2) there are significant differences in metacognitive and critical thinking skills between pre-service elementary school teachers when integrated with field independence cognitive style and field dependence cognitive style; (3) there is an interaction between discovery learning and discussion method integrated with cognitive styles on metacognitive and critical thinking skills of pre-service elementary school teachers.
ChroPack - ProteinA/G v1
Authors: Ehl Alexandra , Frommholz David , Stefanczyk Nadine
Published: December 2019

Purification Guide for the Isolation of Antibodies with ChroPack Columns by DALEX Biotech. With ChroPack Protein A/G columns you can quickly isolate monoclonal and polyclonal antibodies from various sample materials. Antibodies bind with high specificity to the recombinant protein G whose BSA binding site has been removed. The ChroPack columns can be operated with peristaltic pumps and common FPLC systems. The Luer-Lock connections make changing the column particularly easy. Each ml bed volume has a binding capacity of > 15 mg (tested with polyclonal human Ig, the binding capacity varies according to species and clones). Up to 98 % purity in a single purification step. No swelling/shrinking of the matrix in aqueous buffers. High thermal stability, up to 15 minutes at 80 °C in aqueous buffers with neutral pH. pH stability: short-term pH 2 - 8, long-term pH 3 - 8. Suitable for all common low-pressure systems.
Protocol Griess Test v1
Authors: Silva Tamiris , de Oliveira Rodini Carolina , Kalil Bussadori Sandra , de Brito Sousa Kaline , Porta Santos Fernandes Kristianne
Published: December 2019

To assess nitrite levels in individuals with relapsing-remitting multiple sclerosis, the Griess test was performed. A collection of 10 mL of peripheral blood was performed. Nitrite is the only stable end product of nitric oxide self-oxidation in aqueous solution. A high correlation has been established between endogenous NO production and plasma, serum and urine nitrite / nitrate levels. Therefore, the measurement of these levels provides a reliable and quantitative estimate of the nitric oxide output. Serum nitrite levels are measured by reacting the samples with the 96 well microplate Griess reagent. The total nitrite will be analyzed by reacting the samples with 50 μl Griess reagent. Total nitrite is estimated from a standard absorbance curve on a 592 nm reader.
Normative Values for Thumb Length in Young Adult Population
Authors: MUTLUAY Şükriye Deniz
Published: December 2019

Aim: This study was conducted to identify normal values for thumb length with respect to the index finger. Material and Method: Measurements were taken from dorsal and palmer sides of a totally 164 right and left hands of 82 healthy young adults (47 males and 35 females) aged between 18 and 21 years. Measurements of relative thumb length, girth and nail width were performed with respect to the index finger. Measurement values were classified as Thumb Proximal Phalanx (TPP) index and Thumb Digital Crease (TDC) index.  Results: Average values of the TPP and TDC indexes were found 0.82 and 0.59 respectively for right hand and 0.74 and 0.49 respectively for left hand. There is no statistically significant differences indicated between right and left hand averages in terms of gender. Average percentage values of thumb length with respect to proximal phalanx of index finder (AD/AB) were found to be 74% and 77% in right hand for females and males respectively; and 70% and 73% in left hand for males and females respectively. Average percentage values of the thumb length with respect to the length of the whole index finger (AD/AC) were found to be 35% and 34% in right hand and left hand respectively. Comparison of the thumb girth and nail width to index finger girth and nail width indicated the percentage values as 132% and 106% respectively. Conclusion: The provided data could help to the clinician’s as to detect pathologies and analyse aesthetic outcomes of reconstructive surgeries intraoperatively and postoperatively.
Oral L-Karnitin Kullanan Hastada Tedavi Yaklaşımımız
Authors: KOYUNCU Onur , URFALI Senem , POLAT Sibel , HAKİMOĞLU Sedat , ÖZBAKIŞ AKKURT Çağla
Published: December 2019

L-Karnitinin vücutta, kas dokusunun önemli bir elemanıdır. Zayıflama ve egzersiz performansını arttırıcı etkisinden dolayı özellikle genç erişkinlerde son 30 yılda popüler bir ilaç olmuştur.İlaç olarak kullanıldığında, aşırı dozlarında ciddi istenmeyen etkileri ortaya çıkmaktadır. Biz bu olgu sunumunda, L-Karnitin kullanımına bağlı nörolojik yan etkiler gelişen bir hastada yoğun bakımdaki tedavi yaklaşımımızı sunduk.
Akciğer Nakil Adaylarında Egzersiz Kapasitesinin Periferik Kas Kuvveti Ve Solunum Fonksiyonları İle İlişkisi
Authors: PEHLİVAN Esra , KALAFAT Sevda , BALCI Arif , KILIÇ Lütfiye
Published: December 2019

Amaç: Çalışmamızda akciğer nakil adaylarında fonksiyonel egzersiz kapasitesi seviyesinin, periferik kas gücü ve solunum fonksiyonları ile olan ilişkisini incelemeyi amaçladık.Gereç ve Yöntem: Hastanemiz pulmoner rehabilitasyon merkezinde, akciğer nakli öncesi rehabilitasyon programına alınması için refere edilen 25 hasta çalışmaya dahil edildi. Hastalar 6 -dakika yürüyüş testi yürüme mesafelerine (6DYM) göre 2 gruba ayrıldı (Grup1: 6DYM<350m, n:14; Grup2: 6DYM≥350m, n=11). Grupların kas ve yağ kütle ve yüzdeleri, periferik kas kuvvetleri karşılaştırıldı, egzersiz kapasitesi seviyesinin kas kütlesi ve solunum fonksiyonları ile olan ilişkisi incelendi. Bulgular: Gruplar benzer demografik özelliklere sahipti (yaş, beden kitle indeksi). Kas kütleleri (p=,956), yağsız doku yüzdeleri (p=,956), yağ kütleleri (p=,827) ve vücut yağ yüzdeleri (p=,701) benzerdi. Periferik kas kuvvetleri karşılaştırıldığında diz ekstansiyonu, dirsek fleksiyonu ve el kavrama kuvvetleri benzer olup, Grup1’in dorsifleksiyon kas kuvvetinin (p=,039) ve FEV1% (p=0,033) değerlerinin istatistiksel olarak daha düşük olduğu tespit edildi. 6DYM ile diğer sonuç ölçümleri arasında yapılan ilişki analizinde dirsek fleksiyonu (r=,483; p=,015), dorsifleksiyon (r=,518; p=,008) kas kuvveti ve FEV1% (r=,514; p=,009) ile orta, FVC% (r=,605; p=,001)  ile ise yüksek seviyede korelasyon tespit edildi.Sonuç: Çalışmamızdaki akciğer nakil adayı olan hastaların egzersiz kapasiteleri, kas ve yağ kütlesinden bağımsız olup, kas kuvvetinden ve solunum fonksiyonlarından etkilenmektedir. Bu durum, bu hasta grubunda egzersiz kapasitesindeki azalmanın daha ziyade primer akciğer patolojisine bağlı olduğunu düşündürmektedir. Daha anlamlı sonuçların elde edilebilmesi için, rehabilitasyon sonrası değişimler incelenmelidir.
Lazer Doppler Flovmetre
Authors: GÜLER Derya , ŞEN TUNÇ Emine
Published: December 2019

Pulpa
vitalitesinin değerlendirilmesi diş hekimliği pratiğinde teşhis aşamasında
kritik bir öneme sahiptir. Geleneksel pulpa canlılık test yöntemleri pulpal
innervasyona dayalıdır ve bazen yanlış pozitif ya da yanlış negatif cevaplar
verebilmektedir. Ayrıca, bu yöntemler hastanın uyaranlara cevabını
değerlendirdiklerinden subjektif testlerdir. Pulpa vitalitesi, kan desteğinin
doku içinde bulunduğu anlamına gelmektedir. Son zamanlarda pulpa vitalitesinin
belirlenmesinde pulpal kan akımının yarı nicel kaydına izin veren non invaziv
ve ağrısız elektro optik bir teknik olan Lazer Doppler Flovmetre’nin
kullanılmaya başlandığı görülmektedir. Bu derleme ile dokuların canlılığının
değerlendirilmesinde non-invaziv bir yöntem olan Lazer Doppler Flovmetre
cihazının anlatılması ve bu yöntemin kullanılabileceği alanların bildirilmesi
amaçlanmıştır.
Bir Toplum Ruh Sağılığı Merkezinde İzlenen Hastaların Klinik ve Sosyodemografik Özellikleri ile Tedavi yaklaşımlarının incelenmesi
Authors: ŞAHPOLAT Musa
Published: December 2019

Amaç: Bu çalışmada amaç bir Toplum Ruh Sağlığı Merkezi’nden hizmet alan hastaların klinik ve sosyodemografik özellikleri ile tedavi yaklaşımlarının incelenmesidir.Metod: Toplum ruh sağlığı merkezinde kayıtlı ve çalışmaya katılmayı kabul eden toplam 203 hastayla klinisyen yüz yüze görüşme yaparak DSM-IV tanı kriterlerine göre hastalara tanı konuldu. Kesitsel tipte olan çalışmada tüm hastalara sosyodemografik veri formu, bipolar bozukluk grubu hastalarına ise Hamilton Depresyon Derecelendirme Ölçeği (HDDÖ) ve Young Mani Derecelendirme Ölçeği (YMDÖ), psikotik bozukluk grubu hastalarına ise Pozitif ve Negatif Sendrom Ölçeği (PANSS) uygulandı.Bulgular: Çalışmamızda 52(%25,6) hastada bipolar bozukluk tanısı, 151 (%74,4) hastada psikotik bozukluk tanısı vardı. Hastaların ölçek puan ortalamaları HDDÖ 3,40±1,62, YMDÖ 2,38±0,97 ve PANSS Toplam 58,94±12,16 idi. Hastaların çoğunluğu erkek olup hanedeki ilk ve ikinci çocuklarda ruhsal hastalığın daha fazla oranda görüldüğü belirlenmiştir. 78 (%38,4) hastanın birinci derecede yakınlarında psikiyatrik hastalığın olduğu, bunun yanında 24 (%11,8) hastada adli sorun öyküsünün ve 4 (%2) hastada madde kötüye kullanımı öyküsünün olduğu görülmüştür. Tedaviye bakıldığında hastaların 29 (%14,3) kişisi risperidon uzun etkili enjeksiyon formu, 6 (%3) kişisi paliperidon palmitat uzun etkili enjeksiyon formu ve 9 (%4,4) kişisi aripiprazol uzun etkili enjeksiyon formu kullanmaktaydı.Sonuç: Bildiğimiz kadarıyla çalışmamız Türkiye’de Toplum Ruh Sağlığı Merkezi’nden hizmet alan hastaların klinik ve tedavi özelliklerini inceleyen nadir çalışmalardan biridir. Bu nedenle çalışmamızın literatür açısından önemli olduğu düşünmekteyiz.
Apiksaban Kullanımı Sonucu Gelişen Psoas Kası Kanamasına Bağlı Akut Böbrek Yetmezliği: Olgu Sunumu
Authors: DİBEK Yusuf , KARAKILIÇ Olgun , TURGUT Faruk Hilmi
Published: December 2019

Oral bir antikoagülan olan Apiksaban valvüler olmayan atrial fibrilasyonlu hastalarda inme veya sistemik embolik olayların önlenmesinde sıkça kullanılmaktadır. Aktif bir faktör Xa inhibitörü olan Apiksaban parsiyel tromboplastin zamanı (PTT), protrombin zamanı(PTZ) değerlerini uzatabilmektedir. Tüm antikoagülanlarda olduğu gibi Apiksaban kullanımı kanama riskini arttırır. Psoas kasına kanama nadir görülen fakat ciddi bir komplikasyondur. Bu olgu sunumunda Apiksaban’ın kullanımı sonucu spontan gelişen psoas kası kanamasına bağlı gelişen akut böbrek yetmezliğini sunmayı amaçladık.
INVESTIGATION OF THERMAL AND STRUCTURAL PROPERTIES OF NITRIC, HYDROCHLORIC AND SULPHURIC ACID-TREATED ZEOLITE
Authors: AKBELEN Meryem , EZBER Çağatay
Published: December 2019

In this study, in order to
consider the effect of the hydrochloric acid (HCl), nitric acid (HNO3)
and sulphuric acid (H2SO4) treatments on
thermal and structural properties, zeolite was modified with 1 M
acid solutions at 70 oC during 4 h using batch method. The
structural and thermal properties of zeolites were studied by X-ray diffraction
(XRD), X-ray fluorescence (XRF), thermogravimetric analysis (TG), differential
thermogravimetric analysis (DTG) and differential thermal analysis (DTA). Quantitative
XRD analysis show that the natural zeolite mainly consists of mordenite and
clinoptilolite with varying amounts of quartz, feldspar and clay mineral. TG-DTG
and DTA curves of all zeolite samples were obtained in the temperature range
between 30 and 1000 oC. Based on the TG results, the
total mass losses for all acid-treated samples (8.51-8.44 %) are less than that
of the natural (Z) sample (10.14 %).  SiO2/
Al2O3 ratio of the natural zeolite sample (Z) increased
from 5.6 to 9.8, 9.7 and 8.8 in the Z-N, Z-H and Z-S samples, respectively,
indicating the removal of the significant amount of aluminum from the zeolite
structure.
Divulgación de la experiencia de un modelo de utilidad de transferencia, apropiación y adopción tecnológica de siembra bajo invernadero en productores rurales caso: El Edén de Cris del corregimiento de La Elvira del municipio de Cali
Authors: Banguero Camacho Clara Viviana , López Rodríguez Sergio Andrés
Published: December 2019

El artículo muestra los resultados del estudio cuyo propósito es divulgar la experiencia de la transferencia y apropiación tecnológica del modelo de utilidad en la siembra bajo invernadero mediante la aplicación de la metodología investigación social, caso  El Edén de Cris, corregimiento de La Elvira, período 2012 - 2016.  Los resultados revelan que al aplicar la metodología en el proyecto,  se logró la transferencia tecnológica, la apropiación y adopción de siembra bajo invernadero por medio de un modelo de utilidad; sin embargo, la continuidad del proyecto involucrando más productores agrícolas generaría un impacto socioeconómico en la localidad. El modelo de utilidad permitió aplicar nuevas formas de producción agrícola a través de las Unidades Productivas Agrícolas Sostenibles, la replica a más productores podría contribuir a disminuir las brechas de exclusión social e inequidad existentes en la localidad de la Elvira, reconfigurar el territorio a partir del diseño de  un micro-circuito productivo sostenible con el agroecosistema, mediante  la construcción social de relaciones productivas en redes de productores rurales.
Maternal and fetal outcomes with the use of prostaglandins E2 as a cervical ripening agent for induction of labour
Authors: Gupta Shagun , Kuntal Neha , Gupta Virendra Kumar
Published: December 2019

Background: In modern medicine induction of labour is required in patients for a good feto-maternal outcome. PGE2 is a prostaglandin analogue which has been used as a cervical ripening agent to improve bishops score. Objective of this study was to evaluate the efficacy of intravaginal PGE2 gel as a cervical ripening agent in unfavourable cervix for induction of labor and any complications associated with its use.Methods: This study comprised of 90 women who required labor induction. Singleton pregnancy above 37 weeks, live intrauterine fetus, Cephalic presentation, Bishop score of 1-6, reactive FHR pattern were included. Women who required only single induction were categorized as Group 1. Those requiring more than one dose after reassessment of bishops scoring at 6, 12 and 18 hours belonged to Group 2.Results: Group1 had more of younger population below 30 years consisting more primigravidas with > 80% women having gestational age of > 39 weeks. Most common indication for induction of labour in both groups was post-dated pregnancy. 65 patients received one dose of cerviprime gel forming Group 1. In Group 2, 72% received 2 doses and 28%, 3 doses of gel. Initial bishops score mean was 4.2 in Group 1 and 4.1 in Group 2. Mean change in bishop score was analysed after 6, 12, and 18 hours of instillations of PGE2 gel. Significant p value was obtained in all groups requiring one, two and three doses of gel. In Group 1, 12.3% and in Group 2, 16% had LSCS. Maternal side effects were minimal and neonatal outcome was good.Conclusions: The study showed that intravaginal application of PGE2 is effective, safe and acceptable method as a cervical ripening agent for labor induction in women with poor bishops score. It reduces caesarean delivery rate without increasing maternal and neonatal morbidity.
A MULHER EXECUTIVA NA ATUALIDADE: A CONSTRUÇÃO DA LÍDER FEMININA * EXECUTIVE WOMEN IN THE PRESENT: THE CONSTRUCTION OF THE FEMALE LEADER
Authors: Carmona Garcia Manzano Luciana , Do Nascimento Gomes Adilson
Published: December 2019

Este artigo busca observar o funcionamento do discurso da mídia empresarial na construção da imagem da mulher executiva na contemporaneidade, momento histórico-social configurado pela conquista de um espaço de liderança para a mulher, mas também momento em que o discurso machista atravessa essa conquista e transforma o espaço em lugar de luta pelo direito de ser mulher e empresária. Para tanto, analisamos duas reportagens da revista empresarial HSM Management, direcionadas aos profissionais do mundo corporativo, que tratam da mulher de negócios, a partir dos estudos em Análise do Discurso, especialmente das reflexões de Michel Foucault. Os resultados mostram que a hegemonia histórica construída sobre o homem como líder executivo ainda pauta a construção da mulher no meio empresarial.*This paper seeks to observe the operation of the business media discourse in the construction of the executive woman image in contemporaneity, a social-historical moment configured by the conquest of a leadership space for women, but also at a time when the sexist discourse crosses this conquest and changes the space into a fighting place for the right to be a woman and a businesswoman. Therefore, we have analyzed two articles in the business magazine HSM Management, aimed at professionals in the corporate world, who deal with businesswomen, based on the studies in Discourse Analysis, especially from Michel Foucault's reflections. The results show that the historical hegemony built on the man as executive leader still guides the construction of women in the business environment.
REPRESENTAÇÕES DO COMPORTAMENTO FEMININO EM BELO HORIZONTE (1920-1930) * REPRESENTATIONS OF FEMALE BEHAVIOUR IN BELO HORIZONTE (1920-1930)
Authors: Pereira Lucas Carvalho Soares de Aguiar
Published: December 2019

Os impressos contribuíram para a difusão de representações, de diferenciações e de classificações sociais do comportamento feminino. Durante as décadas de 1920 e 1930 em Belo Horizonte, Minas Gerais, os jornais publicaram diversos textos dispersos dirigidos às mulheres e aos seus comportamentos que são uma importante série documental para a análise das distinções a partir da interseccionalidade do gênero com as dimensões raciais e sexuais (morais). Essas representações produziram e foram produzidas pela dicotomia entre “mulher honesta” e “mulher decaída”. Argumento que essa situação é parte de um amplo processo de construção de diferentes mecanismos de normatização dos comportamentos femininos que se relacionam com as distinções sociais e de gênero que marcaram a sociedade brasileira.*The press contributed to the production and dissemination of social representation, differentiation and classifications of female behaviour. The 1920’s and 1930’s newspapers in Belo Horizonte, Minas Gerais, Brazil, have published several dispersed texts addressed to women and their behaviour. They form an important series of documents to the analysis of social distinctions from intersectionality of gender with the racial, social and sexual (moral) dimensions. These representations forged and have been shaped by the dichotomy between the “honest woman” and the “fallen woman”. I argue that this situation is part of a large process of production of different arrangements of standardisation of female performance that are related with the social and gender distinctions of the Brazilian society.
"MULHERES TREMENDAS": A VIOLÊNCIA FEMININA NO RIO GRANDE DO SUL (1889-1930) * “TREMENDOUS WOMEN”: THE FEMALE VIOLENCE IN RIO GRANDE DO SUL (1889-1930)
Authors: Barbosa Carla Da Silva
Published: December 2019

O presente artigo pretende explorar processos-crime da Fronteira Oeste do Rio Grande do Sul durante a Primeira República (1889-1930) em que mulheres agrediram outras mulheres, seus amásios ou ex-amásios.  Pretende-se analisar de que forma estas manifestações são consideradas apropriadas ou não pela sociedade, bem como os motivadores de sua hostilidade física e/ou verbal. Através do enquadramento legal destas ocorrências, dos termos utilizados nos documentos e nos depoimentos dos envolvidos, pretende-se demonstrar como a investigação da violência feminina requer o questionamento não apenas do conceito de violência, mas dos papéis atrelados aos gêneros nas sociedades.*The present paper intends to explore six criminal processes of the Frontier of Rio Grande do Sul during the First Republic (1889-1930) in which women attacked other women, their lovers, exlovers and police officers. The intention is to analyze how these manifestations are considered appropriate or not by society, as well as the motivators of their physical or verbal hostility. Through the legal framework of these occurrences, the terms used in the documents and statements of those involved; we aim to demonstrate how the investigation of female violence requires the questioning not only of the concept of violence but of gender roles in societies.
A VIOLÊNCIA NAS RELAÇÕES DE GÊNERO ATRAVÉS DOS CASOS DE RAPTOS CONSENTIDOS NA PARAÍBA NAS DÉCADAS DE 1920-1930 * VIOLENCE IN GENDER RELATIONS THROUGH THE CASES OF CONSENTED ABDUCTIONS IN PARAIBA IN THE DECADES OF 1920- 1940
Authors: Santana Rosemere Olimpio
Published: December 2019

O presente artigo pretende discutir como a violência esteve atrelada as relações de gênero na Paraíba especificamente na prática dos raptos consentidos. O rapto era comum não só na Paraíba como no Brasil, era acionado quando por algum motivo não era permitido o relacionamento entre o casal apaixonado e era realizado com o consentimento da raptada. Mesmo assim, a prática do rapto consentido em diversos documentos e representações da época elencam o lugar do masculino como protagonizante das histórias e nos ajudam a problematizar os vários lugares de violência que dependendo do documento e da ocasião eram legitimados ou criticados. Para análise desse artigo trabalhamos com cordéis, processos-crimes e música.*This paper intends to discuss how violence was linked to gender relations in Paraíba, specifically in the practice of consenting abduction. The kidnapping was common not only in Paraíba but also in Brazil, it was triggered when for some reason the relationship between the couple in love was not allowed and was carried out with the abducted consent. Even so, the practice of consented abduction in several documents and representations of the time list the place of the male as the protagonist of the stories and help us to problematize the various places of violence that depending on the document and the occasion was legitimized or criticized. For analysis of this article, we work with cordel, crimeprocesses, and music in the decades of 1920-1940.
REGIONALISMO CENTRÍFUGO: A CONSTRUÇÃO DE UM “NORDESTE PERNAMBUCANO” NA DÉCADA DE 1920 * CENTRIFUGAL REGIONALISM: THE CREATION OF A “NORDESTE PERNAMBUCANO” IN THE 1920S
Authors: Cavalcante Júnior Idelmar Comes
Published: December 2019

Muito já foi dito sobre a invenção do Nordeste como uma construção imagético-discursiva. Tomando tal interpretação, já consagrada, como premissa fundamental, este trabalho tem como objetivo mostrar de que forma a elaboração da região Nordeste pelos intelectuais regionalistas pernambucanos na década de 1920, procurou frequentemente qualificar Pernambuco como o centro da região nordestina. Assim, o estado foi sendo constituído como um centro irradiador responsável não apenas pelo despertar de uma consciência regionalista, mas também por defender e consolidar a causa regionalista. Desta forma, se regionalismo nordestino, de forma mais explícita, questionava o federalismo brasileiro da época, considerado centrífugo por prestigiar apenas dois ou três estados, os intelectuais de Pernambuco que participaram desta luta pareciam propor, sub-repticiamente, um regionalismo igualmente centrífugo.*A lot of things have been said about the invention of the Northeast of Brazil as an imagediscursive creation. Taking such established interpretation as a fundamental premise, this paper intends to show in which way the elaboration of the northeastern region of Brazil by regionalist intellectuals from Pernambuco in the 1920s sought often to qualify their State as the Center of the region. Thus, this State has been constituted as a radiating Center responsible for not only the awakening of a regionalist consciousness, but also to defend and consolidate the regionalist cause. Therefore, if the northeastern regionalism questioned the contemporary Brazilian Federalism, in a more explicit way, considering it centrifugal for benefiting only two or three States, the intellectuals from Pernambuco who participated in this fight seemed to propose, surreptitiously, an equally centrifugal regionalism.
DEMANDAS COLETIVAS DE TRABALHO DE JUIZ DE FORA (MG) E A ATUAÇÃO DO TRIBUNAL REGIONAL DO TRABALHO DA 3ª REGIÃO DURANTE A PRIMEIRA DÉCADA DA DITADURA MILITAR BRASILEIRA (1964-1974) * COLLECTIVE WORK ACTIONS IN JUIZ DE FORA (MG) AND THE PERFOMANCE OF “TRIBUNAL REGIONAL DO TRABALHO DA 3ª REGIÃO” DURING THE FIRST DECADE OF THE BRAZILIAN CIVIL-MILITARY DICTATORSHIP (1964-1974)
Authors: Damião Paulo Henrique Silveira
Published: December 2019

Busca-se refletir sobre a atuação dos trabalhadores e sindicatos de Juiz de Fora na Justiça do Trabalho durante o contexto de autoritarismo da ditadura militar brasileira, especificamente na sua primeira década (1964-1974). O uso da justiça trabalhista como instrumento para se buscar a garantia de direitos e melhores condições de trabalho em um contexto de autoritarismo se revela através da análise dos autos processuais dos processos coletivos impetrados pelas representações dos trabalhadores da referida cidade, tramitados no Tribunal Regional do Trabalho da 3ª Região (TRT3). Pretende-se captar as experiências dos trabalhadores nas negociações coletivas e a relação entre suas demandas e o contexto político, social e econômico do período, refletindo sobre os impactos dos autoritarismos de Estado nas relações de trabalho e procurando perceber se este tribunal atuou no sentido de buscar reparar as perdas de direitos trabalhistas e amenizar os ataques do regime autoritário direcionados aos trabalhadores.*We seek to reflect on the workers’ performance and unions of Juiz de Fora in the “Justiça do Trabalho” during the context of authoritarianism of the Brazilian civil-military dictatorship, specifically in its first decade (1964-1974). The use of “Justiça do Trabalho” as an instrument to seek the guarantee of rights and better working conditions in a context of authoritarianism is revealed through the analysis of the procedural documents of the collective lawsuits filed by the representatives of the workers of that city, processed in the Regional Labor Court of the 3rd Region (TRT3). The aim is to capture the experiences of workers in collective bargaining and the relationship between their demands and the political, social and economic context of the period, reflecting on the impacts of state authoritarianism on labor relations and trying to understand if this court acted in the sense of repairing the losses of labor rights and to soften the attacks of the authoritarian regime directed to the workers.
DE DEBATES HISTÓRICOS-POLITÍCOS À CONSTRUÇÕES DE SI: A NARRATIVA DO BRASIL NA AUTOBIOGRAFIA DE SIMONE DE BEAUVOIR * FROM HISTORICAL-POLITICAL DEBATES TO SELF-CONSTRUCTIONS: THE NARRATIVE OF BRAZIL IN SIMONE DE BEAUVOIR’S AUTOBIOGRAPHY
Authors: Cardinalli Thainã Teixeira
Published: December 2019

Em 1960, Simone de Beauvoir (1908-1986), acompanhada de Jean-Paul Sartre, visitou as principais cidades do sudeste, nordeste e centro-oeste brasileiro na companhia dos seus anfitriões Jorge Amado e Zélia Gattai. Os relatos dessa viagem integram o terceiro volume de sua autobiografia intitulada A Força das Coisas (1963). Neste artigo investigo a composição de suas descrições do Brasil em estreito diálogo com duas dimensões narrativas: os debates históricos-políticos que marcaram a sua estadia no país, tais quais as noções de “Terceiro Mundo” e de países subdesenvolvidos, e os movimentos de independências das ex-colônias europeias; e os projetos de construção de si elaborados nas suas obras autobiográficas; gênero textual que será apresentado com o auxílio dos estudos teóricos de Philippe Lejeune (1991) e Leonor Arfuch (2010).*In 1960, Simone de Beauvoir (1908-1986) and Jean-Paul Sartre visited the main cities in the southeast, northeast and central-west Brazilian regions, accompanied by their hosts in the country, Jorge Amado and Zélia Gattai. This trip reports are part of Beauvoir's autobiography, namely its third volume, entitled A Força das Coisas (1963). In this paper, my goal is to inquire the composition of the philosopher's descriptions and reports of Brazil in a narrow dialogue with two narrative dimensions: the historical-political debates that marked her time in the South-American country, such as the concepts of "Third-World", underdeveloped countries, and the independence movements of the former European colonies; as well as the projects of Simone de Beauvoir's self-representation in her autobiographic works; literary genre that will be addressed with the aid of Philippe Lejeune (1991) and Leonor Arfuch’s (2010) theoretical studies.
UM “ARQUITETO MUITO HÁBIL” PARA O IMPÉRIO: POLÍTICA, ESTADO E CONSTRUÇÃO DA NAÇÃO NO PENSAMENTO VARNHAGENIANO * A “VERY SKILLED ARCHITECT” FOR THE EMPIRE: POLITICS, STATE AND CONSTRUCTION OF THE NATION IN VARNHAGENIAN THOUGHT
Authors: Sá Ana Priscila De Sousa
Published: December 2019

O artigo teceu alguns comentários sobre a conjuntura política do Império do Brasil de meados do século XIX, as posições políticas e o papel do Estado na obra de Francisco Adolfo de Varnhagen. Monarquista assumido e afinado com as ideias de conservação da ordem e centralização do governo, para o historiador paulista, o Estado era o principal instrumento de construção da nação. Num momento de consolidação do poder imperial no Segundo Reinado, Varnhagen, como outros letrados e estadistas, escreveram pensando num futuro promissor para o Brasil.*In this paper, we elaborated some comments about the political conjuncture of the Brazilian Empire in the mid-nineteenth-century, the political positions and the role of the State in Francisco Adolfo de Varnhagen’s work. A self-recognized monarchist and in tune with the ideas of conservation of the order and centralization of the government, for the historian of São Paulo, the State was the main instrument of construction of the nation. At a moment of consolidation of imperial power in the Second Reign, Varnhagen, like other scholars and statesmen, wrote for a promising future for Brazil.
APRESENTAÇÃO AO DOSSIÊ HISTÓRIA E GÊNERO: NOVOS DEBATES
Authors: Atallah Gianne Zanella , Matos Júlia Silveira
Published: December 2019

Texto de Apresentação do Dossiê História e Gênero: novos debates
Indocyanine green as a prospective sensitizer for photodynamic therapy of melanomas.
Authors: Urbanska Krystyna , Romanowska-Dixon Bozena , Matuszak Zenon , Oszajca Janusz , Nowak-Sliwinska Patrycja , Stochel Grazyna
Published: December 2019

Spectroscopic, photochemical and biological properties of indocyanine green (ICG) are presented. Light over 800 nm is effectively absorbed by ICG. This property as well as photochemical behaviour of ICG make it a very suitable dye for photodynamic treatment of melanoma cells. Cytotoxicity of ICG itself and the effect of photodynamic therapy (PDT) were evaluated by following the growth of human (SKMEL 188) and mouse (S91) melanoma cells. The surviving fraction of the cells irradiated (lambda(ex) = 830 nm) vs non-irradiated, treated with the same dose of ICG, is significantly decreased (5- to 10-fold). These results show that ICG is a very promising dye for photodynamic therapy of melanomas.
The structure of the oligosaccharides of alpha3beta1 integrin from human ureter epithelium (HCV29) cell line.
Authors: Lityńska Anna , Pocheć Ewa , Hoja-Lukowicz Dorota , Kremser Elzbieta , Laidler Piotr , Amoresano Angela , Monti Chiara
Published: December 2019

There is a growing line of evidence that glycosylation of alpha and beta subunits is important for the function of integrins. Integrin alpha3beta1, from human ureter epithelium cell-line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrometry. Our findings demonstrated, in both subunits of integrin alpha3beta1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi- tri- and tetraantennary structures were the most common, while high-mannose type structures were minor. Also the presence of short poly-N-acetyllactosamine entities was shown. These results show that while the predominant oligosaccharides of both subunits are identical, some slight differences between them do exist.
Nitrate reduction and nitrogen fixation in symbiotic association Rhizobium-legumes.
Authors: Luciński Robert , Polcyn Władysław , Ratajczak Lech
Published: December 2019

The inhibitory effect of nitrate on nitrogenase activity in root nodules of legume plants has been known for a long time. The major factor inducing changes in nitrogenase activity is the concentration of free oxygen inside nodules. Oxygen availability in the infected zone of nodule is limited, among others, by the gas diffusion resistance in nodule cortex. The presence of nitrate may cause changes in the resistance to O2 diffusion. The aim of this paper is to review literature data concerning the effect of nitrate on the symbiotic association between rhizobia and legume plants, with special emphasis on nitrogenase activity. Recent advances indicate that symbiotic associations of Rhizobium strains characterized by a high nitrate reductase activity are less susceptible to inhibition by nitrate. A thesis may be put forward that dissimilatory nitrate reduction, catalyzed by bacteroid nitrate reductase, significantly facilitates the symbiotic function of bacteroids.
A comparison of the in vitro genotoxicity of anticancer drugs idarubicin and mitoxantrone.
Authors: Błasiak Janusz , Gloc Ewa , Warszawski Mariusz
Published: December 2019

Idarubicin is an anthracycline antibiotic used in cancer therapy. Mitoxantrone is an anthracycline analog with presumed better antineoplastic activity and lesser toxicity. Using the alkaline comet assaywe showed that the drugs at 0.01-10 microM induced DNA damage in normal human lymphocytes. The effect induced by idarubicin was more pronounced than by mitoxantrone (P < 0.001). The cells treated with mitoxantrone at 1 microM were able to repair damage to their DNA within a 30-min incubation, whereas the lymphocytes exposed to idarubicin needed 180 min. Since anthracyclines are known to produce free radicals, we checked whether reactive oxygen species might be involved in the observed DNA damage. Catalase, an enzyme inactivating hydrogen peroxide, decreased the extent of DNA damage induced by idarubicin, but did not affect the extent evoked by mitoxantrone. Lymphocytes exposed to the drugs and treated with endonuclease III or formamidopyrimidine-DNA glycosylase (Fpg), enzymes recognizing and nicking oxidized bases, displayed a higher level of DNA damage than the untreated ones. 3-Methyladenine-DNA glycosylase II (AlkA), an enzyme recognizing and nicking mainly methylated bases in DNA, increased the extent of DNA damage caused by idarubicin, but not that induced by mitoxantrone. Our results indicate that the induction of secondary malignancies should be taken into account as side effects of the two drugs. Direct strand breaks, oxidation and methylation of the DNA bases can underlie the DNA-damaging effect of idarubicin, whereas mitoxantrone can induce strand breaks and modification of the bases, including oxidation. The observed in normal lymphocytes much lesser genotoxicity of mitoxantrone compared to idarubicin should be taken into account in planning chemotherapeutic strategies.
Polymorphism in intron 23 of the endothelial nitric oxide synthase gene (NOS3) is not associated with hypertension.
Authors: Derebecka Natalia , Hołysz Marcin , Dankowski Rafał , Wierzchowiecki Michał , Trzeciak Wiesław H
Published: December 2019

Nitric oxide (NO) is synthesised in the vascular endothelium by nitric oxide synthase (NOS3) and is an important factor in the regulation of blood pressure. Impaired synthesis of NO due to mutations in the NOS3 gene is associated with hypertension. To date several allelic variants of the NOS3 gene have been identified and their possible linkage with hypertension investigated. We studied the distribution of genotypes and frequency of alleles of the G11T polymorphism in intron 23 of the NOS3 gene in patients with hypertension and in a control group of healthy individuals. The polymorphism was determined by PCR-RFLP analysis. The distribution of genotypes in the patients with hypertension and in the healthy individuals did not differ significantly from the values predicted from Hardy-Weinberg equilibrium for the general population. No major differences in the distribution of the G11T polymorphism in the patients and healthy individuals were found (P > 0.05).
Thermodynamics of specific protein-RNA interactions.
Authors: Stolarski Ryszard
Published: December 2019

Description of the recognition specificity between proteins and nucleic acids at the level of molecular interactions is one of the most challenging tasks in biophysics. It is key to understanding the course and control of gene expression and to the application of the thus acquired knowledge in chemotherapy. This review presents experimental results of thermodynamic studies and a discussion of the role of thermodynamics in formation and stability of functional protein-RNA complexes, with a special attention to the interactions involving mRNA 5' cap and cap-binding proteins in the initiation of protein biosynthesis in the eukaryotic cell. A theoretical framework for analysis of the thermodynamic parameters of protein-nucleic acid association is also briefly surveyed. Overshadowed by more spectacular achievements in structural studies, the thermodynamic investigations are of equal importance for full comprehension of biopolymers' activity in a quantitative way. In this regard, thermodynamics gives a direct insight into the energetic and entropic characteristics of complex macromolecular systems in their natural environment, aqueous solution, and thus complements the structural view derived from X-ray crystallography and multidimensional NMR. Further development of the thermodynamic approach toward interpretation of recognition and binding specificity in terms of molecular biophysics requires more profound contribution from statistical mechanics.
Spread and survival of promiscuous IncP-1 plasmids.
Authors: Adamczyk Małgorzata , Jagura-Burdzy Grazyna
Published: December 2019

Plasmids classified to the IncP-1 incompatibility group belong to the most stably maintained mobile elements among low copy number plasmids known to date. The remarkable persistence is achieved by various tightly controlled stability mechanisms like active partitioning, efficient conjugative transfer system, killing of plasmid-free segregants and multimer resolution. The unique feature of IncP-1 plasmids is the central control operon coding for global regulators which control the expression of genes involved in vegetative replication, stable maintenance and conjugative transfer. The multivalent regulatory network provides means for coordinated expression of all plasmid functions. The current state of knowledge about two fully sequenced plasmids RK2 and R751, representatives of the IncP-1alpha and IncP-1beta subgroups, is presented.
Overproduction and purification of the CcpA protein from Lactococcus lactis.
Authors: Kowalczyk Magdalena , Bardowski Jacek
Published: December 2019

In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xl1blue strain as a fusion with 6 x His tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 microg/ml ampicillin at 37 degrees C and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.
Carrier-state of D allele in ACE gene insertion/deletion polymorphism is associated with coronary artery disease, in contrast to the C677-->T transition in the MTHFR gene.
Authors: Zak Iwona , Niemiec Paweł , Sarecka Beata , Balcerzyk Anna , Ciemniewski Zbigniew , Rudowska Ewa , Dylag Stanisław
Published: December 2019

Angiotensin I-converting enzyme (ACE), which plays an important role in blood pressure regulation, and methylenetetrahydrofolate reductase (MTHFR) involved in homocysteine metabolism belong to a large group of polypeptides which may be potential risk factors for atherosclerosis and coronary artery disease (CAD). To assess whether polymorphisms of the genes encoding these peptides are associated with CAD in Silesian we conducted a study among 68 individuals suffering from CAD (including 52 cases after myocardial infarction), 51 subjects with positive family history of CAD and 111 controls. We analysed the distribution of genotypes and allele frequencies of the insertion/deletion (I/D) polymorphism in the ACE gene using PCR amplification, and the C677-->T polymorphism in the MTHFR gene using PCR-RFLP analysis. We found that D allele frequency was significantly higher in CAD patients (61%) than in controls (43%) (P = 0.001, OR = 2.06). The D allele carriers (DD + ID genotypes) were more frequent in the CAD patients (85%) compared to control group (65%) (P = 0.003, OR = 3.14), whereas the familial CAD risk group shows the highest frequency of the ID genotype (57% vs 43% in controls). In contrast, the MTHFR polymorphism does not seem to be associated with the disease. Our data indicate that in Silesian CAD patients the disease is strongly associated with carrier-state of the ACE D allele, but not with the C677-->T transition in the MTHFR gene.
Expression of a constitutively active mutant of heat shock factor 1 under the control of testis-specific hst70 gene promoter in transgenic mice induces degeneration of seminiferous epithelium.
Authors: Widłak Wiesława , Benedyk Konrad , Vydra Natallia , Głowala Magdalena , Scieglińska Dorota , Małusecka Ewa , Nakai Akira , Krawczyk Zdzisław
Published: December 2019

Heat shock activates in somatic cells a set of genes encoding heat shock proteins which function as molecular chaperones. The basic mechanism by which these genes are activated is the interaction of the specific transcription factor HSF1 with a regulatory DNA sequence called heat shock element (HSE). In higher eukaryotes HSF1 is present in unstressed cells as inactive monomers which, in response to cellular stress, aggregate into transcriptionally competent homotrimers. In the present paper we showed that the expression of a transgene encoding mutated constitutively active HSF1 placed under the control of a spermatocyte-specific promoter derived from the hst70 gene severely affects spermatogenesis. We found the testes of transgenic mice to be significantly smaller than those of wild-type males and histological analysis showed massive degeneration of the seminiferous epithelium. The lumen of tubules was devoid of spermatids and spermatozoa and using the TUNEL method we demonstrated a high rate of spermatocyte apoptosis. The molecular mechanism by which constitutively active HSF1 arrests spermatogenesis is not known so far. One can assume that HSF1 can either induce or repress so far unknown target genes involved in germ cell apoptosis.
Effect of anthocyanins on selected biochemical parameters in rats exposed to cadmium.
Authors: Kowalczyk Edward , Kopff Anna , Fijałkowski Paweł , Kopff Maria , Niedworok Jan , Błaszczyk Jan , Kedziora Józef , Tyślerowicz Piotr
Published: December 2019

Cadmium is a dangerous occupational and environmental toxin. It accumulates in the human organism mainly in liver and kidneys. Cadmium half-life is about 10 years, so the symptoms of cadmium intoxication may occur several years after the exposure. Until now in treating intoxication with this metal chelating compounds have been used, burdened with numerous undesirable symptoms. In our investigations anthocyanins from Aronia melanocarpa were used to reduce the harmful results caused by cadmium. Administering anthocyanins with cadmium chloride resulted in a statistically significant decrease of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity, concentration of bilirubin and urea in blood serum and decreased cadmium cumulation in liver and kidneys in relation to animals receiving cadmium chloride only.
Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base/nucleoside.
Authors: Foksinski Marek , Gackowski Daniel , Rozalski Rafał , Olinski Ryszard
Published: December 2019

We assessed a relationship between the level of 8-oxodG in leukocyte DNA measured with the high performance liquid chromatography with electrochemical detection (HPLC/EC) technique and urinary excretion of the modified nucleoside/base analysed with a recently developed methodology involving HPLC prepurification followed by gas chromatography with isotope dilution mass spectrometric detection. No correlation was found between these markers of oxidative DNA damage commonly used in epidemiological studies. Several possible explanations of this finding are discussed.
ON THE BEGINNING OF MODERN INDONESIAN LITERATURE
Authors: Nugraha Dipa , Suyitno
Published: December 2019

Purpose of the study: This study aims to bridge the gap in the dispute over the beginning of modern Indonesian literature and determine which literary work marked the beginning of modern Indonesian literature. Methodology: This uses close reading over selected texts on the usage of specific words in Malay used in anti-colonialism political movement and the existence of the words Indonesia, bangsa, negeri, tanah air, and tumpah darah as the central themes for the development of the analysis from the selected texts. The selected texts are four novels published in the Dutch East Indies in the early of twentieth century: Student Hidjo (1919), Hikajat Kadiroen (1920), Azab dan Sengsara (1920), and Sitti Nurbaja (1922). Main Finding: We find that Azab dan Sengsara and Sitti Nurbaja have the word Indonesia and help conceptualize the new idea of the homeland. Whilst Student Hidjo and Hikajat Kadiroen do not see beyond the land of Java and they only relate the Dutch East Indies to the land of Java. We argue that Sitti Nurbaja marked the beginning of modern Indonesian literature. Implications: The debate over the beginning of modern Indonesian literature should stop with the finding of this study that Azab dan Sengsara and Sitti Nurbaja mark the beginning of modern Indonesian literature. While Student Hidjo and Hikajat Kadiroen must be named as the modern pre-Indonesian literary works.