bluuebunny/crossref_metadata_2025 huggingface dataset:
Mangrove pneumatophore arthropod assemblages and temporal patterns
Authors: Procheş Ş. , Marshall D.J. , Ugrasen K. , Ramcharan A.
Published: April 2017

Arthropod assemblages comprising mites, crustaceans and hexapods are characterized here for mangrove pneumatophores in south-eastern Africa. Initial sampling showed that pneumatophore assemblages differed markedly from benthic sediment assemblages, not only in being more species rich, but also in having lower abundances. Differences among pneumatophore arthropod assemblages were observed in comparisons of two mangrove stands (in the Durban region) and habitats within each stand. Strikingly higher arthropod abundances were found in assemblages associated with pneumatophores on the seaward fringes of the mangrove stands, as compared to those situated inside the mangrove stands or along minor waterways. These differences in abundance are ascribed to differences in physical conditions among habitat types, relating to wetting frequency and sunlight exposure. The assemblages associated with minor waterways varied among themselves according to variations in salinity. Temporal variation in abundance showed that some species peaked in summer and others in winter, indicating effects more closely related to terrestrial seasonal patterns, than to seawater temperature. This study highlights the uniqueness of the mangrove pneumatophore arthropod assemblages, and the need for further investigation into these in order to better understand mangrove meiofaunal ecology.
Temporal and spatial co-occurrence in spawning and larval release of Cliona viridis (Porifera: Hadromerida)
Authors: Mariani S. , Piscitelli M.P. , Uriz M.-J.
Published: April 2017

This is the first report of egg release by the oviparous excavating sponge Cliona viridis. Adult specimens of excavating (α) and massive (β) sponge forms for the presence of oocytes were monitored from 11 May to 12 July 2000, in a shallow-rocky coast of the north-west Mediterranean. The immediate environment around the sponge was sampled for the presence of eggs. Spawning occurred synchronously in the study area at temperatures above 19°C. Oocytes were released in clusters enclosed in adhesive maternal tissue. They contained symbiotic zooxanthellae. Free, adhesive egg-masses drifted in the water or adhered to erect algae in the vicinity of the sponge. Morula stages and larval release are described.
Seasonal digestive gland dynamics of the scallop Pecten maximus in the Bay of Brest (France)
Authors: Pennec Gaël Le , Pennec Marcel Le , Beninger G.
Published: April 2017

Cytological and biochemical changes in the digestive gland of Pecten maximus throughout a one-year period were investigated in the Bay of Brest (France) relative to lipid storage and glycogen and α-amylase concentrations in tubuloacinar terminations. Seasonal variations in both cell structure and biochemical composition were observed. These changes were independent of the tidal cycle, but correspond to phytoplankton abundance. From November to January, cells of digestive acini were hypertrophied due to the high lipid storage. From February to May, these reserves were markedly reduced, cell size decreased, and the acinar lumen was clearly apparent. From May to September, the RNAs transcribing for α-amylase increased, and a correlation was found between digestion events and lipid storage in acinar cells. The relationships between metabolite transfers from digestive gland to gonad and other tissues are described. Distinct lipid storage sites appear to be associated with maintenance energy and acute demand energy, such as gametogenesis when adductor muscle reserves have been depleted. The digestive gland may thus function as a relay organ during periods of energetic stress, notably during the first gametogenesis in February and March and for shell growth in early spring.
Enzymatic activity in Mytilus galloprovincialis (Mollusca: Bivalvia) digestive gland
Authors: Pastore M. , De Lisi A. , Biandolino F. , Marzulli D.
Published: April 2017

Mitochondrial dehydrogenases, antioxidant enzymes, transaminases and cytochrome P450 activity have been studied from the digestive gland of Mytilus galloprovincialis, reared in a non-polluted area of the Mar Grande of Taranto (Ionian Sea, Mediterranean).
Ultrastructural localization and antibacterial activity of phenoloxidase in amphioxus Branchiostoma belcheri tsingtauense
Authors: Li Guorong , Zhang Shicui , Li Hongyan
Published: April 2017

Phenoloxidase (PO) has been shown to be present in both invertebrates and vertebrates, yet little is known about the PO in Branchiostoma belcheri tsingtauense. The present study demonstrated that the PO activity existed histochemically the epithelial cells of the gill bar and intestine of adult animal, producing brown to black melanin deposits. Ultrastructural examination revealed that PO reaction products were localized in the cytoplasmic granules of the epidermal cells, the epithelial cells of gill bar and intestine and most of them were electron-dense and homogenous. Frequently, the PO reaction products were also observed in the secondary lysosomes.  The mucus from the body surface had little antibacterial activity against Escherichia coli, but it enhanced the antibacterial activity of L-dopa and the enhancement was suppressed by PTU, a PO specific inhibitor. This indicates that the active material inhibiting the E. coli growth in the mucus is PO.
Stomach contents of two Mediterranean monk seals (Monachus monachus) from the Aegean Sea, Turkey
Authors: Salman Alp , Bilecenoglu Murat , Güçlüsoy Harun
Published: April 2017

This study provides a description of the Mediterranean monk seal (Monachus monachus) diet from the Aegean Sea coast of Turkey. A total of 23 prey items belonging to five species were identified from the two stomachs examined. Cephalopods constitute the dominant prey group by weight (94·01%). Sarcotragus sp. (Porifera) and Posidonia oceanica (Magnoliophyta) are assumed to be incidental prey. Of the cephalopods identified, Eledone moschata and Bathypolypus sponsalis were encountered for the first time in a monk seal stomach.
On Being Stuck in Bengal: Immobility in the ‘age of migration’
Authors: CHATTERJI JOYA
Published: April 2017

Immobility raises awkward questions for theorists of migration. From their standpoint, migration is unusual behaviour that requires explanation. Its obverse—staying in place—is seen as an ‘obvious’ state of affairs that calls for no explanation. Yet assumptions about the ordinariness of immobility are insecure. For one thing, we know a great deal more about the mobile societies of early modern Asia; for another, Asian mobility in the era of high imperialism is much better understood. Yet despite these cumulative gains in our understanding of the scale of mobility in early-modern and modern Asia, and its acceleration in ‘the age of migration’, immobility continues to be seen as the ‘obvious’ state of affairs. This article suggests some preliminary answers to ‘the immobility paradox’, based on a study of the greater Bengal region. By analysing the impact of the intensifying links, in the late colonial era, between Bengal and the global economy, it shows that this varied widely for different people, in ways that had a profound bearing on their capacity to move. The article develops the notion of ‘deficits’ which worked to inhibit the mobility of particular groups and individuals. Physical frailty and obligations of care, it shows, were crucial factors in shaping immobility. Relations of gender and generation, and the inequalities embedded in these relations, produced ‘overabundances’—of obligations to people and places—that tied certain people down. Finally, it hints at the reasons why, and the ways in which, stayers-on have grown poorer.
The Biology of Disease Vectors, 2nd Edn (ed. Marquardt, W. C.), pp. 785. Elsevier Academic Press, USA, 2005. ISBN 0 12 473276 3. £62.99.
Authors: HURD HILARY
Published: April 2017

Vector biology has become a fast moving field. Spurred on by the recent exciting advances in the molecular biology, genomics and latterly proteomics of vector insects and their pathogens, researchers are using new approaches and our knowledge is rapidly increasing. The second edition of this volume very much reflects these advances. The editor, William Marquardt, has assembled a team of section editors to oversee the production of 57 short chapters, divided into 7 parts. With a few exceptions, these experts are drawn from the USA, a reflection no doubt of the history of the first edition which was produced to accompany the Biology of Vector Disease course, given at Colorado State University. The text is aimed at post-graduate and post-doctoral researchers, working in a range of areas associated with vector biology and vector-borne diseases. It provides an excellent opportunity to obtain an initial overview of a new area or to dip into a field that may be peripheral to the topic under investigation.
The chromosomes of Picea sitchensis (Bong.) Carr. and its relatives
Authors: Fox D. P.
Published: April 2017

Synopsis

Picea sitchensis has a haploid complement of twelve metacentric or submetacentric chromosomes which are similar to each other in size. Prominent secondary constrictions, which are probably the sites of nucleolus organisers, occur on five chromosomes (N.O.R.s). The DNA content of the haploid, unreplicated chromosome set is 9 × 109 nucleotide pairs and, in spite of earlier claims, probably does not vary significantly between different populations. B-chromosomes occur extensively in the southern half of the species range. They have little effect on growth rate but delay the time of ♂ and ♀ flowering. Meiosis starts and ends in spring, taking about twenty-two days to complete. The B-chromosomes do not pair at meiosis and appear to be distributed at random. There is marked accumulation of B-chromosomes when transmitted through the ♀ parent, possibly due to preferential segregation during meiosis. A balance between gain by accumulation and loss due to delay of flowering may account for the natural distribution of the B-chromosome.
British willows; some hybrids and some problems
Authors: Meikle R. D.
Published: April 2017

Synopsis

The paper deals with three problem areas in the taxonomy of British willows: (1) the widespread occurrence in Perthshire and neighbouring counties of an intermediate betweenSalix myrsinifoliaSalisb. andS. phylicifoliaL., apparently of hybrid origin, though growing in an area where typicalS. phylicifoliahas yet to be found; (2) the complex taxonomy of the British Sallows, where two common aggregate species,Salix cinerea L. andS. caprea L., are each represented by two segregates; a third,S. aurita L., may also comprise more than one segregate; all three species interbreed, as do the segregates within each aggregate; (3) the disputed identity of the Crack Willow,Salix fragilis L., and its nomenclatural implications.
Assessment of Risks Posed to VAD Patients During Disasters
Authors: Davis Katherine J , O’Shea Genevieve , Beach Michael
Published: April 2017

Ventricular assist devices (VADs) are an Advanced Life Support for patients with heart failure. These patients are particularly vulnerable in the event of a disaster. A hazard vulnerability analysis (HVA) was conducted to determine areas of susceptibility for these patients. Lack of electrical power, limited access to medications and anticoagulation, dehydration, extreme temperature and weather environments, conditions which predispose to infection, and evacuation transport are all identified circumstances that place these patients at an increased risk for harm and death. Future preparations in disaster planning are needed to address and mitigate these risks.

DavisKJ,O’SheaG,BeachM.Assessment of risks posed to VAD patients during disasters.Prehosp Disaster Med.2017;32(4):457–461.
Genetic correlations among body condition score, somatic cell score, milk production, fertility and conformation traits in dairy cows
Authors: Kadarmideen H. N.
Published: April 2017

Genetic and phenotypic parameters for body condition score (BCS), days to first service (DFS), non-return rate (NRR), somatic cell score (SCS), and 305-day milk, fat and protein yields were estimated, using 38930 multiple lactation records of cows across 1830 herds. The cows were daughters of '243 different sires. Single- and multi-trait repeatability animal models were used to estimate parameters based on restricted maximum likelihood methodology. Fixed effects in the model varied depending on the individual trait. Further, genetic relationships between 27 (linear and descriptive) type traits and functional traits (fertility and SCS) were estimated by regressing daughter type records on their sire's estimated breeding values for functional traits, using the same data set. Estimated regression coefficients were then subsequently used to derive approximate genetic correlations between type and functional traits (DFS, NRR and SCS). Body condition score had a moderate heritability (h2) of 0-26 and fertility traits had a low h2 (0-12 for DFS and 0-06 for NRR). Heritability of SCS and milk production traits was 0-14 and around 0-30, respectively. Ratio of permanent environmental variance to phenotypic variance was higher than h2 for all traits considered and ranged from 0-09 for NRR to 0-45 for milk yield. Body condition score had a favourable genetic correlation (rg) with SCS, but it was not strong (-0-08). Milk production was genetically antagonistic with fertility (rg: range 0-12 to 0-27 with DFS and -0-12 to -0-24 with NRR), with BCS (-0-39 to -0-50), and with SCS (0-10 to 0-15) showing that selecting for milk production alone would lead to decline in genetic merit for these functional traits. Body condition score had favourable genetic correlations with fertility traits (-0-35 with DFS and 0-04 with NRR) suggesting that BCS could be considered in a fertility index. Several type traits (especially 'udder' traits) had a favourable genetic relationship with fertility traits and SCS whereas dairy character had an unfavourable genetic relationship. Results of this study on genetic and phenotypic correlations among several functional, type and production traits could be used to design improved selection indexes for dairy producers.
Metabolic adaptation and hormonal regulation in young rabbit does during long-term caloric restriction and subsequent compensatory growth
Authors: Rommers J. M. , Boiti C. , Brecchia G. , Meijerhof R. , Noordhuizen J. P. T. M. , Decuypere E. , Kemp B.
Published: April 2017

An experiment was performed to assess the metabolic adaptation and hormonal regulation in young female rabbits during long-term food restriction and subsequent compensatory growth during rearing. Feeding level was either ad libitum (AL, no. = 52) or restricted (R, no. = 52). From 6 to 12 weeks of age, food intake ofR was kept at a constant level. This resulted in an increase in relative restriction as compared with AL to 0-54of AL intake at 12 weeks of age (restriction period). Thereafter food intake gradually increased to 0-95 of AL at 17 weeks of age (recovery period). During the last 5 days before insemination at 17-5 weeks of age, all animals were fed to appetite. Blood samples were taken weekly from 6 to 17 weeks of age from 11 animals in each group. Growth rate of R was reduced during the restricted period (29 (s.d. 2) v. 44 (s.d. 5) g/day for R and AL, respectively; P 0-05), but was higher in the recovery period (30 (s.d. 3) v. 27 (s.d. 4) g/day, respectively; P<0-05). At first insemination, AL rabbits were heavier than R (4202 (s.d. 388) v. 3798 (s.d. 220) g, respectively; P < 0-001). During the restricted period, plasma glucose was constantly lower (P < 0-05) in R. Insulin levels paralleled those of glucose, being lower (P < 0-05) in R than in AL. Restriction reduced (P < 0-05) circulating corticosterone and tri-iodothyronine (T3) levels in R. Leptin, non-esterified fatty acids, and plasma urea nitrogen levels were similar for AL and R during food restriction, whereas triglycerides were similar until 10 weeks of age, after which the levels were lower in R. During the recovery period, the food intake of the R but not AL rabbits increased. Insulin was the only hormone in R rabbits that had returned to levels found in AL rabbits by the 2nd week of the recovery period. Glucose, T3, and corticosterone levels returned to levels found in AL rabbits between 3 to 4 weeks after refeeding. Non-esterified fatty acids, triglycerides, and leptin were higher (P < 0-05) in AL rabbits from 13 weeks of age onwards. The pattern of changes in the endocrine status during food restriction and compensatory growth in rabbits do conform with those from other species, although some specific changes may vary depending on the severity of food restriction and its duration.
Endogenous amino acid flows at the terminal ileum of broilers, layers and adult roosters
Authors: Ravindran V. , Hendriks W. H.
Published: April 2017

Endogenous flows of nitrogen and amino acids at the terminal ileum of broilers (6 weeks old), layers (70 weeks old) and adult roosters (70 weeks old) were determined using the peptide alimentation method. The ileal endogenous output of nitrogen and total amino acids in broilers, layers and roosters, expressed as mg/kg dry matter intake, were similar (F > 0-05). Endogenous flows were similar (F > 0-05) for nine of the 17 amino acids analysed, but the flows of serine, glutamic acid, proline, alanine, isoleucine, tyrosine, arginine and methionine differed (P < 0-05) among the classes of chickens. The amino acid profile of endogenous protein, expressed asg/100 g crude protein, did not differ (F > 0-05) between the three classes of chickens, except for serine, glutamic acid, proline and isoleucine. The concentrations of proline were higher (F < 0-05) in broilers, compared with the other two groups. The concentrations of glutamic acid in layers were lower (F < 0-05) than the other two groups. The concentrations of serine and isoleucine were higher (F < 0-05) in roosters than the other two groups. In all three groups, the most abundant amino acid in the ileal endogenous protein was glutamic acid, followed by aspartic acid, proline, serine, glycine and threonine. The present study provides estimates for endogenous amino acid flow at the terminal ileum in broilers, layers and adult roosters under normal physiological conditions.
Effects of season and parity on performance of lactating sows in a tropical climate
Authors: Gourdine J. L. , Renaudeau D. , Noblet J. , Bidanel J. P.
Published: April 2017

The effects of season in a tropical climate and parity on lactation performance of 106 Large White sows over several reproductive cycles (301 lactations in total) were studied in Guadeloupe (French West Indies, 16°N latitude, 61°W longitude) between January 1999 and March 2003. Two seasons were determined a posteriori from climatic parameters recorded continuously in a station close to the experimental unit. During the warm season, ambient temperature and relative humidity averaged 23-5°C and 0-831, respectively. In the hot season, the corresponding values were 26-0°C and 0-834, respectively. Over the 28-day lactation period, average daily food intake (ADFI) was lower (P < 0-001) and live-weight (LW) loss was higher (P < 0-05) during the hot season than during the warm season (3-9 v. 4-7 kg/day and 17 v. 12 kg, respectively). Growth rate and mean LW of piglets at weaning were lower (P < 0-05) during the hot season (203 v. 220 g/day and 7-0 v. 7-5 kg, respectively). However, the weaning-to-oestrus interval was not affected by season (5-4 days on average). The effect of season on ad libitum ADFI was less accentuated for primiparous than for multiparous sows (warm-hot 360 v. 2002 g/day). Irrespective of the season, ADFI was lower (P < 0-01) and piglet growth rate tended to be lower (P < 0-2) in primiparous than in multiparous sows (4-0 v. 4-4 kg/day and 205 v. 215 g/day, respectively). Moreover, the percentage of sows that returned to oestrus later than 5 days after weaning was higher in primiparous than in multiparous sows (25 v. 2%, P < 0-001). This study confirmed the negative effects of the hot season on performance of lactating sows raised in a humid tropical climate; the effects of season are dependent on parity number.
The effects of mineral-block components when offered to ewes in late pregnancy on colostrum yield and immunoglobulin G absorption in their lambs
Authors: Boland T. M. , Brophy P. O. , Callan J. J. , Quinn P. J. , Nowakowski P. , Crosby T. F.
Published: April 2017

Ninety twin-bearing ewes were given food individually and allocated to five (no. = 18) treatments in order to determine the effects of supplementing their diet in late pregnancy with mineral-block components on colostrum production, lamb serum immunoglobulin G (IgG) concentration and colostral IgG absorption. Ewes were offered grass silage ad libitum, supplemented with 400 to 500 g per ewe per day of concentrates from day 99 of gestation, in addition to receiving one of the following supplements: C, (control) no supplement; B, mineral block; ML, liquid molasses; MN, granular minerals; ML + MN, liquid molasses and granular minerals. The experiment commenced on day 99 of gestation. Ewes were milked at lh, 10 h and 18 h post lambing and all lambs were fed measured quantities of colostrum, proportionate to birth weight, via stomach tube. Treatment had no effect (P > 0-05) on colostrum yield at lh, 10 h or 18 h post partum or on total colostrum yield to 18 h post partum. Ewes offered molasses (ML) or molasses plus minerals (ML + MN) had a lower colostral IgG concentration at lh post lambing than the control ewes (C) (P < 0-05). Ewes offered molasses (ML) also had a lower colostral IgG concentration than the control (C) at 10 h post partum (P < 0-05). Treatment had no effect on total IgG yield to 18 h post partum. When ewes were supplemented with minerals in any combination, with or without molasses (B, MN, ML + MN) it resulted in lambs having an impaired ability to absorb colostral IgG. Lambs from treatments B, MN and ML + MN had significantly poorer efficiency of colostral IgG absorption than lambs born to control ewes (C) or molasses (ML) supplemented ewes (P < 0-001). This in turn resulted in the progeny of mineral supplemented ewes (B, MN or ML + MN) having lower serum IgG concentration at 24 h post partum than either the control (C) or the molasses treatments (ML) (P < 0-001). When ewes were supplemented with molasses only (ML) lamb serum IgG content at 24 h was lower than in lambs born to control (C) ewes (P < 0-05) but this was as a result of a lower intake of colostral IgG (P < 0-05) and not a result of reduced IgG absorption efficiency. In conclusion, the data show that when ewe mineral intake is high in late pregnancy, as was the case in the current experiment, lamb serum IgG concentration and colostral IgG absorption efficiency are reduced. Further work is required to determine which component of the mineral formulation is responsible for this reduced IgG absorption efficiency and the mechanism through which this impaired efficiency operates.
The effect of red clover formononetin content on live-weight gain, carcass characteristics and muscle equol content of finishing lambs
Authors: Moorby J. M. , Fraser M. D. , Theobald V. J. , Wood J. D. , Haresign W.
Published: April 2017

To investigate the effect of red clover formononetin concentration on lamb growth rate and carcass characteristics, 20 lambs (10 ewe lambs and 10 wethers) were grazed on each of three forages : red clover with a high formononetin concentration (HF), red clover with a low formononetin concentration (LF) and a control perennial ryegrass. Animals were finished at condition score 3L, at which point half of all animals were slaughtered immediately, while the other half of the animals were moved to a common ryegrass plot for 3 weeks as a 'withdrawal' period. Mean formononetin concentrations were 0-0, 4-7 and 3-3 g/kg dry matter (DM) for grass, HF and LF swards respectively. The clover swards had higher crude protein concentrations and lower fibre and water-soluble carbohydrate concentrations than the control ryegrass sward. Lambs grazing the HF clover gained 40 g/day live weight more (P < 0-1) than lambs on the other two forages without an increase in forage DM intake as estimated using the nalkane technique. There was no difference in the empty body weight, killing-out proportion, carcass fat class or condition score between animals finished on any of the three forages. Following the 3-week withdrawal period on ryegrass, there were significant residual effects of previous grazed forage on carcass weight, with HF lambs producing heavier carcasses than other lambs. Plasma concentrations of growth hormone and insulin-like growth factor-1 were highest in lambs grazing the HF clover, and suggest a physiological mechanism for the increased growth rates of these animals. There were no differences in the equol contents of the meat of lambs finished on the clover, compared with animals finished on grass, suggesting that there would be no implications for human health following consumption of meat produced from lambs grazing red clover, even with a relatively high concentration of formo'nonetin.
An economic evaluation of long-term sustainability in the dairy sector
Authors: Santarossa J. M. , Stott A. W. , Woolliams J. A. , Brotherstone S. , Wall E. , Coffey M. P.
Published: April 2017

This paper addresses the problem of assigning economic weights to heritable genetic traits of dairy cows while taking all implicit natural resource values into consideration. To do so, a deterministic bio-economic model of a dairy farm enterprise driven by input probabilities of oestrous detection and conception rates that act through the calving interval is constructed. The model further accounts for biologically limiting factors of both livestock and land within a neo-classical economics framework of profit maximization. Departing from the more customary approach of obtaining gross margins to calculate levels of return in the agricultural sector, we employ a natural resource economics methodology where returns are set against an economic point of reference specified as the value of natural assets' productivity and terminal assets' resale value. Introducing the impact of farming intensity on soil fertility enables us to obtain long-run variations in natural asset values as affected by tillage intensity. Results show that economic weights are not constant over the range of changes in genetic improvements due to the non-linearity of the system induced by diminishing marginal product of inputs and finite carrying capacity of resources employed. These values, while invariant to area farmed, are however subject to variations in resource quality and therefore will reflect the sensitivity of long-term sustainability of the system to managerial decisions on intensity of operation. Results further demonstrated that achieving optimum levels of output while precluding the impact of intensity on land productivity can seriously reduce the time horizon over which sustainability can be maintained. Inclusion of the implicit costs of land use on the other hand tended to suggest optimum levels of output below those identified when only considering accounting data.
Use of empirical models that describe the response of lactating dairy cattle to varying ratios of silage to concentrate as described in terms of metabolizable energy intake: the prediction of milk yield and its constituents from a combination of two empirical models
Authors: Woods V. B. , Kilpatrick D. J. , Gordon F. J.
Published: April 2017

Grass silage constitutes a major proportion of the food offered to high producing dairy cows in the western regions of the European Union. In order to achieve optimal milk yield and quality from high genetic merit dairy cows, it is common practice to offer concentrates in addition to grass silage at feeding. The aim of the current study was to combine two existing empirical models in order to allow prediction of the response in milk production to offering varying ratios of grass silage and concentrates to high genetic merit dairy cows, allowing for the fact that grass silage quality can vary. Results have demonstrated that it is possible to predict, with reasonable precision, the milk production response to various combinations of grass silage and concentrates in the diet. This will facilitate investigation of production and economic responses of offering increased levels of concentrates in the diet on milk yield, milk quality and milk value.
An infrared interactive patient position guidance and acquisition control system for use during radiotherapy treatment
Authors: Fuse Hiraku , Komatsu Kenji , Arakawa Hiroyuki , Sakae Takeji , Tatsuya Fujisaki
Published: April 2017

Background

The control of patient position, posture and respiratory movements during radiotherapy is important for effective and specific treatment of malignancy. We have developed an infrared (IR) interactive patient position guidance and acquisition control system for clinical use, comprising IR cameras, IR markers and dedicated software.

Materials and methods

We evaluated the system with ten healthy volunteers and ten experienced operators. IR markers were placed on the body surface. Their positions were calculated using vectors of three translational and three rotational parameters, and the intrafractional error for each marker was acquired with and without respiratory motion. The inclusion of multiple positioning markers allowed for real-time visualisation of the patient posture, with feedback on misalignment and required postural adjustments.

Results

The positioning time was 73 seconds (with a minimum period of 39 seconds), which was significantly shorter than for conventional line alignment. A comparison of positioning reproducibility between conventional line alignment and this system was <3·5 mm and was not patient dependent or operator dependent. An intrafractional error of displacement of up to 10·0 mm was found in the right iliac crest.

Conclusions

This IR interactive system was shown to be high utility and suitable for monitoring patient position, posture and respiratory movements during radiotherapy.
Mysterious "Fairy Circles" Continue to Enchant Scientists
Authors: Witman Sarah
Published: April 2017

Researchers revisit an old theory about the ethereal patterns of vegetation that form in some arid landscapes.
Adaptive Intervention Designs in Pediatric Psychology: The Promise of Sequential Multiple Assignment Randomized Trials of Pediatric Interventions
Authors: Noser Amy E. , Cushing Christopher C. , McGrady Meghan E. , Amaro Christina M. , Huffhines Lindsay P.
Published: April 2017

This article summarizes the utility of multiphase optimization strategy (MOST) and sequential multiple assignment randomized trial (SMART) processes in the development of empirically derived adaptive interventions (AIs). Recent empirical evidence suggests that SMART designs conducted within the context of a MOST framework can be used for building and optimizing AIs and may lead to better clinical care. SMART designs help optimize AIs by determining the best sequencing of decision rules. However, despite their growing relevance, MOSTs and SMARTs are relatively underutilized in the development of pediatric interventions. MOST and SMART designs can be used for developing efficient and cost-effective AIs. Intervention research within the field of pediatric psychology may benefit from incorporating these designs.
VPS35 regulates parkin substrate AIMP2 toxicity by facilitating lysosomal clearance of AIMP2
Authors: Yun Seung Pil , Kim Hyojung , Ham Sangwoo , Kwon Seung-Hwan , Lee Gum Hwa , Shin Joo-Ho , Lee Sang Hun , Ko Han Seok , Lee Yunjong
Published: April 2017

Vacuolar protein sorting-associated protein 35 (VPS35) is involved in retrograde transport of proteins from endosomes to trans-Golgi network. Gene mutations in VPS35 are linked to autosomal dominant late-onset Parkinson’s disease (PD). Although the identification of VPS35 mutations has provided novel insight about its interactions with several PD-associated genes including leucine-rich repeat kinase 2 (LRRK2) and α-synuclein, little information is available about the molecular mechanisms of cell death downstream of VPS35 dysfunction. In this study, we showed that VPS35 has a role in the lysosomal degradation of parkin substrate aminoacyl tRNA synthetase complex-interacting multifunctional protein 2 (AIMP2), of which accumulation leads to poly(ADP-ribose) polymerase-1 (PARP1)-dependent cell death. VPS35 was co-immunoprecipitated with AIMP2, as well as lysosome-associated membrane protein-2a (Lamp2a). Interestingly, this association was disrupted by PD-associated VPS35 mutant D620N. VPS35 overexpression prevented AIMP2-potentiated cell death and PARP1 activation in SH-SY5Y cells. More importantly, knockdown of VPS35 led to PARP1 activation and cell death, which was AIMP2 dependent. These findings provide new mechanistic insights into the role of VPS35 in the regulation of AIMP2 levels and cell death. As AIMP2 accumulation was reported in PD patient’s brains and involved in dopaminergic cell death, identification of VPS35 as a novel regulator of AIMP2 clearance via lysosomal pathway provides alternative venue to control dopaminergic cell death in PD.
Silencing NKG2D ligand-targeting miRNAs enhances natural killer cell-mediated cytotoxicity in breast cancer
Authors: Shen Jiaying , Pan Jie , Du Chengyong , Si Wengong , Yao Minya , Xu Liang , Zheng Huilin , Xu Mingjie , Chen Danni , Wang Shu , Fu Peifen , Fan Weimin
Published: April 2017

NKG2D is one of the major activating receptors of natural killer (NK) cells and binds to several ligands (NKG2DLs). NKG2DLs are expressed on malignant cells and sensitize them to early elimination by cytotoxic lymphocytes. We investigated the clinical importance of NKG2DLs and the mechanism of NKG2DL regulation in breast cancer (BC). Among the NKG2DLs MICA/B and ULBP1/2/3, the expression levels of MICA/B in BC tissues were inversely associated with the Tumor Node Metastasis stage. We first found that the high expression of MICB, but not MICA, was an independent prognostic factor for overall survival in patients with BC. Investigation into the mechanism revealed that a group of microRNAs (miRNAs) belonging to the miR-17-92 cluster, especially miR-20a, decreased the expression of ULBP2 and MICA/B. These miRNAs downregulated the expression of MICA/B by targeting the MICA/B 3’-untranslated region and downregulated ULBP2 by inhibiting the MAPK/ERK signaling pathway. Functional analysis showed that the silencing of NKG2DL-targeting miRNAs in BC cells increased NK cell-mediated cytotoxicity in vitro and inhibited immune escape in vivo. In addition, histone deacetylase inhibitors (HDACis) increased NKG2DL expression in BC cells by inhibiting members of the miR-17-92 cluster. Thus, targeting miRNAs with antisense inhibitors or HDACis may represent a novel approach for increasing the immunogenicity of BC.
Induction of autophagy by spermidine is neuroprotective via inhibition of caspase 3-mediated Beclin 1 cleavage
Authors: Yang Yi , Chen Sicong , Zhang Yuqing , Lin Xiaoxia , Song Yiyin , Xue Zhaoliang , Qian Haoran , Wang Shanshan , Wan Guihua , Zheng Xiaoxiang , Zhang Lihui
Published: April 2017

Spermidine, a natural polyamine presented widely in mammalian cells, has been implicated to extend the lifespan of several model organisms by inducing autophagy. However, the effect of spermidine against neuronal damage has not yet been fully determined. In this study, neuronal cell injury was induced by treating PC12 cells and cortical neurons with 1 μM staurosporine (STS). We found that STS-induced cell injury could be efficiently attenuated by pretreatment with 1 mM spermidine. Spermidine inhibited the caspase 3 activation induced by STS. Moreover, STS incubation resulted in autophagic degradation failure, which could be attenuated by the pretreatment of spermidine. Knocking down the expression of Beclin 1 efficiently suppressed autophagosome and autolysosome accumulation, and abolished the protective effects of spermidine against STS-induced neurotoxicity. Increased Beclin 1 cleavage and partial nuclear translocation of Beclin 1 fragment was detected in STS-treated cells, which could be blocked by spermidine, pan-caspase inhibitor or caspase 3-specific inhibitor. The nuclear translocation of Beclin 1 fragment universally occurs in damaged neurons. Beclin 1 mutation at the sites of 146 and 149 prevented the intracellular re-distribution of Beclin 1 induced by STS. In addition, intraperitoneal injection of spermidine ameliorated ischemia/reperfusion-induced neuronal injury in the hippocampus and cortex of rats, possibly via blocking caspase 3 activation and consequent Beclin 1 cleavage. Our findings suggest that caspase 3-mediated Beclin 1 cleavage occurs in acute neuronal cell injury both in vitro and in vivo. The neuroprotective effect of spermidine may be related to inhibition of the caspase 3-mediated Beclin 1 cleavage and restoration of the Beclin 1-dependent autophagy.
miR-10a suppresses colorectal cancer metastasis by modulating the epithelial-to-mesenchymal transition and anoikis
Authors: Liu Yankun , Zhang Yingnan , Wu Haidong , Li Yufeng , Zhang Yi , Liu Min , Li Xin , Tang Hua
Published: April 2017

MicroRNAs (miRNAs) have a critical role in tumorigenesis and metastasis, which are major obstacles of cancer therapy. However, the role of miRNAs in colorectal cancer (CRC) metastasis remains poorly understood. Here, we found that miRNA-10a (miR-10a) was upregulated in primary CRC tissues and cell line (SW480) derived from primary CRC compared with metastatic cancer tissues in lymph node and cell line (SW620). The differential expression of miR-10a was inversely correlated with distant metastasis and invasion depth. miR-10a promoted migration and invasion in vitro but inhibited metastasis in vivo by regulating the epithelial-to-mesenchymal transition and anoikis. Furthermore, matrix metalloproteinase 14 (MMP14) and actin gamma 1 (ACTG1) were validated as target genes of miR-10a in CRC cells. Ectopic expression of MMP14 and ACTG1 counteracted the decreased cell adhesion and anoikis resistance activities induced by miR-10a. These findings not only describe the mechanism by which miR-10a suppresses CRC metastasis but also suggest the potential prognostic and therapeutic value of miR-10a in CRC patients.
Pd-catalysed ligand-enabled carboxylate-directed highly regioselective arylation of aliphatic acids
Authors: Zhu Yan , Chen Xiaolan , Yuan Chunchen , Li Guobao , Zhang Jingyu , Zhao Yingsheng
Published: April 2017

α-amino acids bearing aromatic side chains are important synthetic units in the synthesis of peptides and natural products. Although various β-C-H arylation methodologies for amino acid derivatives involving the assistance of directing groups have been extensively developed, syntheses that directly employ N-protected amino acids as starting materials remain rare. Herein, we report an N-acetylglycine-enabled Pd-catalysed carboxylate-directed β-C(sp3)-H arylation of aliphatic acids. In this way, various non-natural amino acids can be directly prepared from phthaloylalanine in one step in good to excellent yields. Furthermore, a series of aliphatic acids have been shown to be amenable to this transformation, affording β-arylated propionic acid derivatives in moderate to good yields. More importantly, this ligand-enabled direct β-C(sp3)-H arylation could be easily scaled-up to 10 g under reflux conditions, highlighting the potential utility of this synthetic method.
The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger
Authors: Keightley Maria-Cristina , Carradice Duncan P. , Layton Judith E. , Pase Luke , Bertrand Julien Y. , Wittig Johannes G. , Dakic Aleksandar , Badrock Andrew P. , Cole Nicholas J. , Traver David , Nutt Stephen L. , McCoey Julia , Buckle Ashley M. , Heath Joan K. , Lieschke Graham J.
Published: April 2017

In response to infection and injury, the neutrophil population rapidly expands and then quickly re-establishes the basal state when inflammation resolves. The exact pathways governing neutrophil/macrophage lineage outputs from a common granulocyte-macrophage progenitor are still not completely understood. From a forward genetic screen in zebrafish, we identify the transcriptional repressor, ZBTB11, as critical for basal and emergency granulopoiesis. ZBTB11 sits in a pathway directly downstream of master myeloid regulators including PU.1, and TP53 is one direct ZBTB11 transcriptional target. TP53 repression is dependent on ZBTB11 cys116, which is a functionally critical, metal ion-coordinating residue within a novel viral integrase-like zinc finger domain. To our knowledge, this is the first description of a function for this domain in a cellular protein. We demonstrate that the PU.1–ZBTB11–TP53 pathway is conserved from fish to mammals. Finally, Zbtb11 mutant rescue experiments point to a ZBTB11-regulated TP53 requirement in development of other organs.
Bio-inspired Murray materials for mass transfer and activity
Authors: Zheng Xianfeng , Shen Guofang , Wang Chao , Li Yu , Dunphy Darren , Hasan Tawfique , Brinker C. Jeffrey , Su Bao-Lian
Published: April 2017

Both plants and animals possess analogous tissues containing hierarchical networks of pores, with pore size ratios that have evolved to maximize mass transport and rates of reactions. The underlying physical principles of this optimized hierarchical design are embodied in Murray’s law. However, we are yet to realize the benefit of mimicking nature’s Murray networks in synthetic materials due to the challenges in fabricating vascularized structures. Here we emulate optimum natural systems following Murray’s law using a bottom-up approach. Such bio-inspired materials, whose pore sizes decrease across multiple scales and finally terminate in size-invariant units like plant stems, leaf veins and vascular and respiratory systems provide hierarchical branching and precise diameter ratios for connecting multi-scale pores from macro to micro levels. Our Murray material mimics enable highly enhanced mass exchange and transfer in liquid–solid, gas–solid and electrochemical reactions and exhibit enhanced performance in photocatalysis, gas sensing and as Li-ion battery electrodes.
Climate variations on Earth-like circumbinary planets
Authors: Popp Max , Eggl Siegfried
Published: April 2017

The discovery of planets orbiting double stars at close distances has sparked increasing scientific interest in determining whether Earth-analogues can remain habitable in such environments and how their atmospheric dynamics is influenced by the rapidly changing insolation. In this work we present results of the first three-dimensional numerical experiments of a water-rich planet orbiting a double star. We find that the periodic forcing of the atmosphere has a noticeable impact on the planet’s climate. Signatures of the forcing frequencies related to the planet’s as well as to the binary’s orbital periods are present in a variety of climate indicators such as temperature and precipitation, making the interpretation of potential observables challenging. However, for Earth-like greenhouse gas concentrations, the variable forcing does not change the range of insolation values allowing for habitable climates substantially.
Strongly bound excitons in anatase TiO2 single crystals and nanoparticles
Authors: Baldini E. , Chiodo L. , Dominguez A. , Palummo M. , Moser S. , Yazdi-Rizi M. , Auböck G. , Mallett B.P.P. , Berger H. , Magrez A. , Bernhard C. , Grioni M. , Rubio A. , Chergui M.
Published: April 2017

Anatase TiO2 is among the most studied materials for light-energy conversion applications, but the nature of its fundamental charge excitations is still unknown. Yet it is crucial to establish whether light absorption creates uncorrelated electron–hole pairs or bound excitons and, in the latter case, to determine their character. Here, by combining steady-state angle-resolved photoemission spectroscopy and spectroscopic ellipsometry with state-of-the-art ab initio calculations, we demonstrate that the direct optical gap of single crystals is dominated by a strongly bound exciton rising over the continuum of indirect interband transitions. This exciton possesses an intermediate character between the Wannier–Mott and Frenkel regimes and displays a peculiar two-dimensional wavefunction in the three-dimensional lattice. The nature of the higher-energy excitations is also identified. The universal validity of our results is confirmed up to room temperature by observing the same elementary excitations in defect-rich samples (doped single crystals and nanoparticles) via ultrafast two-dimensional deep-ultraviolet spectroscopy.
A single biochemical activity underlies the pleiotropy of the aging-related protein CLK-1
Authors: Liu Ju-Ling , Yee Callista , Wang Ying , Hekimi Siegfried
Published: April 2017

The Caenorhabditis elegans clk-1 gene and the orthologous mouse gene Mclk1 encode a mitochondrial hydroxylase that is necessary for the biosynthesis of ubiquinone (UQ). Mutations in these genes produce broadly pleiotropic phenotypes in both species, including a lengthening of animal lifespan. A number of features of the C. elegans clk-1 mutants, including a maternal effect, particularly extensive pleiotropy, as well as unexplained differences between alleles have suggested that CLK-1/MCLK1 might have additional functions besides that in UQ biosynthesis. In addition, a recent study suggested that a cryptic nuclear localization signal could lead to nuclear localization in cultured mammalian cell lines. Here, by using immunohistochemical techniques in worms and purification techniques in mammalian cells, we failed to detect any nuclear enrichment of the MCLK1 or CLK-1 proteins and any biological activity of a C. elegans CLK-1 protein devoid of a mitochondrial localization sequence. In addition, and most importantly, by pharmacologically restoring UQ biosynthesis in clk-1 null mutants we show that loss of UQ biosynthesis is responsible for all phenotypes resulting from loss of CLK-1, including behavioral phenotypes, altered expression of mitochondrial quality control genes, and lifespan.
Targeted Enrichment and Sequencing of Recent Endosymbiont-Host Lateral Gene Transfers
Authors: Dunning Hotopp Julie C. , Slatko Barton E. , Foster Jeremy M.
Published: April 2017

Lateral gene transfer (LGT) from microbial symbionts to invertebrate animals is described at an increasing rate, particularly between Wolbachia endosymbionts and their diverse invertebrate hosts. We sought to assess the use of a capture system to cost-effectively sequence such LGT from the host genome. The sequencing depth of Illumina paired end data obtained with a Wolbachia capture system correlated well with that for an Illumina paired end data set used to detect LGT in Wolbachia-depleted B. malayi (p-value: <2e-16). Using a sequencing depth threshold of two or three standard deviations above the mean, 96.9% or 96.7% of positions, respectively, are predicted in the same manner between the two datasets, with 24.7% or 42.5% of the known 49.0 kbp of LGT sequence predicted correctly, respectively. Prior qPCR results for nuwts showed similar correlations for both datasets supporting our conclusion that oligonucleotide-based capture methods can be used to obtain sequences from Wolbachia-host LGT. However, at least 121 positions had a minority of the reads supporting the endosymbiont reference base call using the capture data, illustrating that sequence reads from endosymbiont-host LGTs can confound endosymbiont genome projects, erroneously altering the called consensus genome, a problem that is irrespective to the sequencing technology or platform.