bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-Nucleus Sequencing of an Entire Mammalian Heart: Cell Type Composition and Velocity
Authors: Wolfien Markus , Galow Anne-Marie , Müller Paula , Bartsch Madeleine , Brunner Ronald M. , Goldammer Tom , Wolkenhauer Olaf , Hoeflich Andreas , David Robert
Published: January 2020

Analyses on the cellular level are indispensable to expand our understanding of complex tissues like the mammalian heart. Single-nucleus sequencing (snRNA-seq) allows for the exploration of cellular composition and cell features without major hurdles of single-cell sequencing. We used snRNA-seq to investigate for the first time an entire adult mammalian heart. Single-nucleus quantification and clustering led to an accurate representation of cell types, revealing 24 distinct clusters with endothelial cells (28.8%), fibroblasts (25.3%), and cardiomyocytes (22.8%) constituting the major cell populations. An additional RNA velocity analysis allowed us to study transcription kinetics and was utilized to visualize the transitions between mature and nascent cellular states of the cell types. We identified subgroups of cardiomyocytes with distinct marker profiles. For example, the expression of Hand2os1 distinguished immature cardiomyocytes from differentiated cardiomyocyte populations. Moreover, we found a cell population that comprises endothelial markers as well as markers clearly related to cardiomyocyte function. Our velocity data support the idea that this population is in a trans-differentiation process from an endothelial cell-like phenotype towards a cardiomyocyte-like phenotype. In summary, we present the first report of sequencing an entire adult mammalian heart, providing realistic cell-type distributions combined with RNA velocity kinetics hinting at interrelations.
Tumors Provoke Inflammation and Perineural Microlesions at Adjacent Peripheral Nerves
Authors: Cohnen Jennifer , Kornstädt Lisa , Hahnefeld Lisa , Ferreiros Nerea , Pierre Sandra , Koehl Ulrike , Deller Thomas , Geisslinger Gerd , Scholich Klaus
Published: January 2020

Cancer-induced pain occurs frequently in patients when tumors or their metastases grow in the proximity of nerves. Although this cancer-induced pain states poses an important therapeutical problem, the underlying pathomechanisms are not understood. Here, we implanted adenocarcinoma, fibrosarcoma and melanoma tumor cells in proximity of the sciatic nerve. All three tumor types caused mechanical hypersensitivity, thermal hyposensitivity and neuronal damage. Surprisingly the onset of the hypersensitivity was independent of physical contact of the nerve with the tumors and did not depend on infiltration of cancer cells in the sciatic nerve. However, macrophages and dendritic cells appeared on the outside of the sciatic nerves with the onset of the hypersensitivity. At the same time point downregulation of perineural tight junction proteins was observed, which was later followed by the appearance of microlesions. Fitting to the changes in the epi-/perineurium, a dramatic decrease of triglycerides and acylcarnitines in the sciatic nerves as well as an altered localization and appearance of epineural adipocytes was seen. In summary, the data show an inflammation at the sciatic nerves as well as an increased perineural and epineural permeability. Thus, interventions aiming to suppress inflammatory processes at the sciatic nerve or preserving peri- and epineural integrity may present new approaches for the treatment of tumor-induced pain.
Targeting CD38-Expressing Multiple Myeloma and Burkitt Lymphoma Cells In Vitro with Nanobody-Based Chimeric Antigen Receptors (Nb-CARs)
Authors: Hambach Julia , Riecken Kristoffer , Cichutek Sophia , Schütze Kerstin , Albrecht Birte , Petry Katharina , Röckendorf Jana Larissa , Baum Natalie , Kröger Nicolaus , Hansen Timon , Schuch Gunter , Haag Friedrich , Adam Gerhard , Fehse Boris , Bannas Peter , Koch-Nolte Friedrich
Published: January 2020

The NAD-hydrolyzing ecto-enzyme CD38 is overexpressed by multiple myeloma and other hematological malignancies. We recently generated CD38-specific nanobodies, single immunoglobulin variable domains derived from heavy-chain antibodies naturally occurring in llamas. Nanobodies exhibit high solubility and stability, allowing easy reformatting into recombinant fusion proteins. Here we explore the utility of CD38-specific nanobodies as ligands for nanobody-based chimeric antigen receptors (Nb-CARs). We cloned retroviral expression vectors for CD38-specific Nb-CARs. The human natural killer cell line NK-92 was transduced to stably express these Nb-CARs. As target cells we used CD38-expressing as well as CRISPR/Cas9-generated CD38-deficient tumor cell lines (CA-46, LP-1, and Daudi) transduced with firefly luciferase. With these effector and target cells we established luminescence and flow-cytometry CAR-dependent cellular cytotoxicity assays (CARDCCs). Finally, the cytotoxic efficacy of Nb-CAR NK-92 cells was tested on primary patient-derived CD38-expressing multiple myeloma cells. NK-92 cells expressing CD38-specific Nb-CARs specifically lysed CD38-expressing but not CD38-deficient tumor cell lines. Moreover, the Nb-CAR-NK cells effectively depleted CD38-expressing multiple myeloma cells in primary human bone marrow samples. Our results demonstrate efficacy of Nb-CARs in vitro. The potential clinical efficacy of Nb-CARs in vivo remains to be evaluated.
Chemical Changes of Graphene Oxide Thin Films Induced by Thermal Treatment under Vacuum Conditions
Authors: Hortigüela María J. , Machado Denise , Bdikin Igor , Neto Victor , Otero-Irurueta Gonzalo
Published: January 2020

Reduction of graphene oxide is one of the most promising strategies for obtaining bulk quantities of graphene-like materials. In this study, graphene oxide was deposited on SiO2 and reduced by annealing at 500 K under vacuum conditions (5 × 10−1 Pa). Here, graphene oxide films as well as their chemical changes upon heating were characterized in depth by X-ray photoelectron spectroscopy, Raman spectroscopy, and scanning electron and atomic force microscopies. From the chemical point of view, the as prepared graphene oxide films presented a large quantity of oxidized functional groups that were reduced to a large extent upon heating. Moreover, residual oxidized sulfur species that originated during the synthesis of graphene oxide (GO) were almost completely removed by heating while nitrogen traces were integrated into the carbon framework. On the other hand, regarding structural considerations, reduced graphene oxide films showed more homogeneity and lower roughness than graphene oxide films.
A Computer Vision System for the Automatic Classification of Five Varieties of Tree Leaf Images
Authors: Sabzi Sajad , Pourdarbani Razieh , Arribas Juan Ignacio
Published: January 2020

A computer vision system for automatic recognition and classification of five varieties of plant leaves under controlled laboratory imaging conditions, comprising: 1–Cydonia oblonga (quince), 2–Eucalyptus camaldulensis dehn (river red gum), 3–Malus pumila (apple), 4–Pistacia atlantica (mt. Atlas mastic tree) and 5–Prunus armeniaca (apricot), is proposed. 516 tree leaves images were taken and 285 features computed from each object including shape features, color features, texture features based on the gray level co-occurrence matrix, texture descriptors based on histogram and moment invariants. Seven discriminant features were selected and input for classification purposes using three classifiers: hybrid artificial neural network–ant bee colony (ANN–ABC), hybrid artificial neural network–biogeography based optimization (ANN–BBO) and Fisher linear discriminant analysis (LDA). Mean correct classification rates (CCR), resulted in 94.04%, 89.23%, and 93.99%, for hybrid ANN–ABC; hybrid ANN–BBO; and LDA classifiers, respectively. Best classifier mean area under curve (AUC), mean sensitivity, and mean specificity, were computed for the five tree varieties under study, resulting in: 1–Cydonia oblonga (quince) 0.991 (ANN–ABC), 95.89% (ANN–ABC), 95.91% (ANN–ABC); 2–Eucalyptus camaldulensis dehn (river red gum) 1.00 (LDA), 100% (LDA), 100% (LDA); 3–Malus pumila (apple) 0.996 (LDA), 96.63% (LDA), 94.99% (LDA); 4–Pistacia atlantica (mt. Atlas mastic tree) 0.979 (LDA), 91.71% (LDA), 82.57% (LDA); and 5–Prunus armeniaca (apricot) 0.994 (LDA), 88.67% (LDA), 94.65% (LDA), respectively.
Appearance and Disappearance of Quasi-Liquid Layers on Ice Crystals in the Presence of Nitric Acid Gas
Authors: Nagashima Ken , Maurais Josée , Murata Ken-ichiro , Furukawa Yoshinori , Ayotte Patrick , Sazaki Gen
Published: January 2020

The surfaces of ice crystals near the melting point are covered with thin liquid water layers, called quasi-liquid layers (QLLs), which play crucial roles in various chemical reactions in nature. So far, there have been many spectroscopic studies of such chemical reactions on ice surfaces, however, revealing the effects of atmospheric gases on ice surfaces remains an experimental challenge. In this study, we chose HNO3 as a model atmospheric gas, and directly observed the ice basal faces by advanced optical microscopy under partial pressure of HNO3 (~10−4 Pa), relevant to those found in the atmosphere. We found that droplets (HNO3-QLLs) appeared on ice surfaces at temperatures ranging from −0.9 to −0.2 °C with an increase in temperature, and that they disappeared at temperatures ranging from −0.6 to −1.3 °C with decreasing temperature. We also found that the size of the HNO3-QLLs decreased immediately after we started reducing the temperature. From the changes in size and the liquid–solid phase diagram of the HNO3-H2O binary system, we concluded that the HNO3-QLLs did not consist of pure water, but rather aqueous HNO3 solutions, and that the temperature and HNO3 concentration of the HNO3-QLLs also coincided with those along a liquidus line.
Ocular Involvement in Friedreich Ataxia Patients and Its Relationship with Neurological Disability, a Follow-Up Study
Authors: Rojas Pilar , Ramírez Ana I. , Hoz Rosa de , Cadena Manuel , Ferreras Antonio , Monsalve Blanca , Salobrar-García Elena , Muñoz-Blanco José L. , Urcelay-Segura José L. , Salazar Juan J. , Ramírez José M.
Published: January 2020

Background: This study compared functional and structural visual changes in Friedreich ataxia (FRDA) patients with healthy controls (HC) and correlated these changes with neurological disability. Methods: Eight FRDA Spanish patients and eight HC were selected from 2014 to 2018. Best corrected visual acuity (BCVA), visual field (VF), optic coherence tomography (OCT), and neurological disability measured by “scale for the assessment and rating of ataxia” (SARA) were taken in a basal exploration and repeated after 6 months. A linear mixed analysis and Bonferroni p-value correction were performed. Results: FRDA baseline and follow-up patients showed statistically significant decreases in BCVA, VF, and OCT parameters compared with the HC. Some of the VF measurements and most of the OCT parameters had an inverse mild-to-strong correlation with SARA. Moreover, the analysis of the ROC curve demonstrated that the peripapillary retinal nerve fiber layer (pRNFL) average thickness was the best parameter to discriminate between FRDA patients and HC. Conclusions: The follow-up study showed a progression in OCT parameters. Findings showed a sequential effect in pRNFL, ganglion cell complex (GCC), and macula. The VF and the OCT could be useful biomarkers in FRDA, both for their correlation with neurological disease as well as for their ability to evaluate disease progression.
Non-Deterministic Semantics for Quantum States
Authors: Jorge Juan Pablo , Holik Federico
Published: January 2020

In this work, we discuss the failure of the principle of truth functionality in the quantum formalism. By exploiting this failure, we import the formalism of N-matrix theory and non-deterministic semantics to the foundations of quantum mechanics. This is done by describing quantum states as particular valuations associated with infinite non-deterministic truth tables. This allows us to introduce a natural interpretation of quantum states in terms of a non-deterministic semantics. We also provide a similar construction for arbitrary probabilistic theories based in orthomodular lattices, allowing to study post-quantum models using logical techniques.
A Survey on Entropy and Economic Behaviour
Authors: Hellman Ziv , Peretz Ron
Published: January 2020

Entropy plays a significant role in the study of games and economic behaviour in several ways. A decision maker faced with an n-fold repetition of a decision-making problem needs to apply strategies that become increasingly complex as n increases. When several players are involved in selecting strategies in interactive games, bounds on the memories and cognitive capacities of the players can affect possible outcomes. A player who can recall only the last k periods of history is said to have bounded recall of capacity k. We present here a brief survey of results of games played by players with different bounded recall capacities, in particular those indicating surprisingly strong relations between memory and entropy in the study of the min-max values of repeated games with bounded recall. In addition, we consider uses of entropy in measuring the value of information of noisy signal structures, also known as experiments. These are represented by stochastic matrices, with the rows representing states of the world and the columns possible signals. The classic ordering of experiments, due to David Blackwell and based on decision-making criteria, is a partial ordering, which has led to attempts to extend this ordering to a total ordering. If a decision maker has a prior distribution over the states, receipt of a signal yields a posterior. The difference between the entropy of a prior and the expected entropy of the set of possible posteriors has been proposed as a natural extension of the Blackwell ordering. We survey this alongside the theory of rational inattention, which posits that, since individuals have limited attention, they do not always follow every single piece of economic news in planning their economic behaviour. By modelling attention limits as finite channel capacity in the sense of Shannon, economists have developed a theory that explains a range of observed economic behavioural phenomena well.
Lab@Home: The Open University of Catalonia Hands-on Electronics Laboratory for Online Engineering Education
Authors: Monzo Carlos , Cobo Germán , Morán José Antonio , Santamaría Eugènia , García-Solórzano David
Published: January 2020

Practical experimentation is essential for electronics learning. As the acquisition of practical and professional competences in full online scenario is concerned, it is very relevant the use of technology that allows students to carry out hands-on experiments. This paper presents Lab@Home, the Open University of Catalonia (Universitat Oberta de Catalunya, UOC, Barcelona, Spain) hands-on electronics laboratory, which allows online students to carry out practical experiments anywhere and anytime at a reduced cost and with good portability characteristics. Lab@Home features and students satisfaction are analyzed during six consecutive semesters in two different subjects by means of a self-administered questionnaire, showing the benefits of using Lab@Home in the acquisition of the subjects contents and gathering how could be improved students learning experience by using this kind of electronics laboratory.
De Novo Transcriptome Identifies Olfactory Genes in Diachasmimorpha longicaudata (Ashmead)
Authors: Tang Liangde , Liu Jimin , Liu Lihui , Yu Yonghao , Zhao Haiyan , Lu Wen
Published: January 2020

Diachasmimoorpha longicaudata (Ashmead, D. longicaudata) (Hymenoptera: Braconidae) is a solitary species of parasitoid wasp and widely used in integrated pest management (IPM) programs as a biological control agent in order to suppress tephritid fruit flies of economic importance. Although many studies have investigated the behaviors in the detection of their hosts, little is known of the molecular information of their chemosensory system. We assembled the first transcriptome of D. longgicaudata using transcriptome sequencing and identified 162,621 unigenes for the Ashmead insects in response to fruit flies fed with different fruits (guava, mango, and carambola). We annotated these transcripts on both the gene and protein levels by aligning them to databases (e.g., NR, NT, KEGG, GO, PFAM, UniProt/SwissProt) and prediction software (e.g., SignalP, RNAMMER, TMHMM Sever). CPC2 and MIREAP were used to predict the potential noncoding RNAs and microRNAs, respectively. Based on these annotations, we found 43, 69, 60, 689, 26 and 14 transcripts encoding odorant-binding protein (OBP), chemosensory proteins (CSPs), gustatory receptor (GR), odorant receptor (OR), odorant ionotropic receptor (IR), and sensory neuron membrane protein (SNMP), respectively. Sequence analysis identified the conserved six Cys in OBP sequences and phylogenetic analysis further supported the identification of OBPs and CSPs. Furthermore, 9 OBPs, 13 CSPs, 3 GRs, 4IRs, 25 ORs, and 4 SNMPs were differentially expressed in the insects in response to fruit flies with different scents. These results support that the olfactory genes of the parasitoid wasps were specifically expressed in response to their hosts with different scents. Our findings improve our understanding of the behaviors of insects in the detection of their hosts on the molecular level. More importantly, it provides a valuable resource for D. longicaudata research and will benefit the IPM programs and other researchers in this filed.
Aesthetic Value of Colluvial Blocks in Geosite-Based Tourist Destinations: Evidence from SW Russia
Authors: Ruban Dmitry A. , Sallam Emad S. , Ermolaev Vladimir A. , Yashalova Natalia N.
Published: January 2020

Nature-based tourism is stimulated by the aesthetic properties of landscapes, and particular elements of the latter determine the overall scenic beauty. Big stones on forested mountain slopes are among such elements. The Partisan Glade geosite-based tourist destination ofthe Western Caucasus in southwestern Russia is distinguished by the occurrence of such stones. Their field investigation (measurements of physical parameters and interpretation of the common criteria of tourist-meaningful beauty) shows that these are essentially blocks (clasts with the size of 1–10 m) of all grades (fine, medium, and coarse blocks) and colluvial origin. The blocks influence on such parameters of scenic beauty as scale, condition, balance, diversity, shape, and uniqueness, and, therefore, these blocks are of aesthetic value. The most important is color and size. Apparently, the presence of these big stones stimulates tourists’ positive emotions. It is recommended to avoid block removal or breaking in the course of road maintenance.
Mapping the Causes of Forest Fires in Portugal by Clustering Analysis
Authors: Meira Castro Ana C. , Nunes Adélia , Sousa António , Lourenço Luciano
Published: January 2020

This paper presents a spatial characterization of the distribution at district level of the forest fire events that occurred in mainland Portugal between 1996 and 2015 and whose causes were investigated. We further examine the breakdown of the causes of these forest fires over this period. Results supported by relevant validated statistics show that of the total fire events recorded, 94.4% were identified as an effective occurrence, of which 22.2% had burned an area greater than 1 ha, and of these only 42.1% were investigated. False alarms or fires without a recorded burning area are more significant in the districts of Aveiro, Lisbon and Porto, the biggest municipalities. Of the fires whose causes were investigated, the largest number of recorded events were in NE regions (49.0%), followed by NW regions (41.7%), and finally in the rest of the country (9.3%). Taking into account the ratio between the investigated fires and the total number of fires and the behavior profile produced for cluster analysis, a different panorama is brought to light, with the center and south regions showing greater effort to investigate the fires. A thorough analysis of the causes and motivations of the ignition of these forest fire occurrences showed that human activity, either deliberate (20.4%) or negligent (29.9%), outweigh natural phenomena (0.6%). Reactivations (14.6%) and Unknown (34.5%) causes decreased as time passed, whereas negligent and deliberate causes increased. However, these results could change if the percentage of unknown information in relation to the origin of the forest fires is considerable. The outcome of this research will support an efficient management related to fire mitigation and suppression including establishing preventive actions to reduce the occurrence of forest fires and emphasize the need to improve the procedure for recording forest fire events in Portugal, especially in relation to identifying their cause.
Sonic Rhetorics as Ethics in Action: Hidden Temporalities of Sound in Language(s)
Authors: Katz Steven B.
Published: January 2020

Sonic rhetorics has become a major area of study in the field of rhetoric, as well as composition and literature. Many of the underlying theories of sonic rhetorics are based on post-Heideggerian philosophy, new materialism, and/or posthumanism, among others. What is perhaps similar across these theories of sonic rhetoric is their “turn” from language and the human in general. This short essay explores sonic rhetorics by examining three temporal dimensions found in language. Specifically, the essay focuses on the more obvious sonic dimensions of time in prosody, and then at deeper levels temporal dimensions in a couple of brief but revealing examples from ancient languages (classical Greek, and Biblical Hebrew). Further, this essay suggests some ways in which time is related to ethics in practice and action. For example, just as time is involved in the continuous creation of our increasingly vast, expanding, infinite but bounded universe, Levinas might say that time is necessary to create the ethical space, or perhaps “hypostasis,” one needs for the possibility to encounter “l’autre”—the Other. Beyond prosody, propriety, even kairos, are hidden temporal dimensions of language that may render sonic rhetorics forms of ethical practice.
Environmental Consciousness and Organic Food Purchase Intention: A Moderated Mediation Model of Perceived Food Quality and Price Sensitivity
Authors: Wang Jianming , Pham Thuy Linh , Dang Van Thac
Published: January 2020

As purchase power and consumption knowledge increase, consumers gradually demand safer and healthier products. In addition, consumers focus greater attention on organic food. This study investigates the relationship between environmental consciousness and organic food purchase intention with the mediating roles of perceived food quality and price sensitivity. The objective is to shed new light on our understanding of consumers’ perceptions and behavioral intentions toward organic food. Using sample data of 518 consumers in different food retailers in China, empirical results show that environmental consciousness has a positive impact on organic food purchase intention. Perceived food quality has a mediating effect on the link between environmental consciousness and organic food purchase intention. Price sensitivity moderates the relationship between perceived food quality and organic food purchase intention. Furthermore, price sensitivity moderates the indirect effect of environmental consciousness on organic food purchase intention through perceived food quality.
Location-Based Social Network’s Data Analysis and Spatio-Temporal Modeling for the Mega City of Shanghai, China
Authors: Khan Naimat Ullah , Wan Wanggen , Yu Shui
Published: January 2020

The aim of the current study is to analyze and extract the useful patterns from Location-Based Social Network (LBSN) data in Shanghai, China, using different temporal and spatial analysis techniques, along with specific check-in venue categories. This article explores the applications of LBSN data by examining the association between time, frequency of check-ins, and venue classes, based on users’ check-in behavior and the city’s characteristics. The information regarding venue classes is created and categorized by using the nature of physical locations. We acquired the geo-location information from one of the most famous Chinese microblogs called Sina-Weibo (Weibo). The extracted data are translated into the Geographical Information Systems (GIS) format, and after analysis the results are presented in the form of statistical graphs, tables, and spatial heatmaps. SPSS is used for temporal analysis, and Kernel Density Estimation (KDE) is applied based on users’ check-ins with the help of ArcMap and OpenStreetMap for spatial analysis. The findings show various patterns, including more frequent use of LBSN while visiting entertainment and shopping locations, a substantial number of check-ins from educational institutions, and that the density extends to suburban areas mainly because of educational institutions and residential areas. Through analytical results, the usage patterns based on hours of the day, days of the week, and for an entire six months, including by gender, venue category, and frequency distribution of the classes, as well as check-in density all over Shanghai city, are thoroughly demonstrated.
Poly(ADP-Ribose) Polymerase-3 Regulates Regeneration in Planarians
Authors: Barghouth Paul G. , Karabinis Peter , Venegas Andie , Oviedo Néstor J.
Published: January 2020

Protein ADP-ribosylation is a reversible post-translational modification (PTM) process that plays fundamental roles in cell signaling. The covalent attachment of ADP ribose polymers is executed by PAR polymerases (PARP) and it is essential for chromatin organization, DNA repair, cell cycle, transcription, and replication, among other critical cellular events. The process of PARylation or polyADP-ribosylation is dynamic and takes place across many tissues undergoing renewal and repair, but the molecular mechanisms regulating this PTM remain mostly unknown. Here, we introduce the use of the planarian Schmidtea mediterranea as a tractable model to study PARylation in the complexity of the adult body that is under constant renewal and is capable of regenerating damaged tissues. We identified the evolutionary conservation of PARP signaling that is expressed in planarian stem cells and differentiated tissues. We also demonstrate that Smed-PARP-3 homolog is required for proper regeneration of tissues in the anterior region of the animal. Furthermore, our results demonstrate, Smed-PARP-3(RNAi) disrupts the timely location of injury-induced cell death near the anterior facing wounds and also affects the regeneration of the central nervous system. Our work reveals novel roles for PARylation in large-scale regeneration and provides a simplified platform to investigate PARP signaling in the complexity of the adult body.
Co-Active Prioritization by Means of Contingency Tables for Analyzing Element-level Bridge Inspection Results and Optimizing Returns
Authors: Oyegbile O. Brian , Chorzepa Mi G.
Published: January 2020

An efficient prioritization of bridge actions such as preventive maintenance, rehabilitation, or replacement (MRR) that accounts for inter-element interactions will optimize a long-term return on investments (ROI) in terms of service life extension. What enables this return is the assignment of “Co-Active” elements. This study develops a methodology based on the concept of “Co-Active elements”. The word, “Co-Active”, is used to represent a small group of elements that act together to improve the Bridge Health Index (BHI). The Co-Active parameters for three major bridge groups in Georgia are presented. To illustrate how the Co-Active model works, 1439 in-service bridges’ Element-Level Bridge Inspection results from the state of Georgia in U.S.A., representing a concrete bridge group with six Co-Active elements, are studied. The analysis results indicate that the overall BHI improves by 20% over the subsequent 20 years when expansion joints are replaced. The effects of Co-Active elements on the BHI predictions are quantifiable and depend on factors such as the timing of MRR, the condition of bridge elements as well as the type of MRR. Furthermore, it is concluded that inter-dependent relationships among Co-Active elements are highly affected by Co-Active coefficients. They increase when the degree of dependency among elements increases. Finally, the proposed bridge Co-Active prioritization analysis accounts for a performance target and associated gaps and thus is able to identify critical elements that affect bridge service life the most.
Transcriptional Expression in Human Periodontal Ligament Cells Subjected to Orthodontic Force: An RNA-Sequencing Study
Authors: Kim Kyunam , Kang Hee Eun , Yook Jong In , Yu Hyung-Seog , Kim Euiseong , Cha Jung-Yul , Choi Yoon Jeong
Published: January 2020

This study was performed to investigate the changes in gene expression in periodontal ligament (PDL) cells following mechanical stimulus through RNA sequencing. In this study, premolars extracted for orthodontic treatment were used. To stimulate the PDL cells, an orthodontic force of 100× g was applied to the premolar (experimental group; n = 11), whereas the tooth on the other side was left untreated (control group; n = 11). After the PDL cells were isolated from the extracted teeth, gene set enrichment analysis (GSEA), differentially expressed gene (DEG) analysis, and real-time PCR were performed to compare the two groups. GSEA demonstrated that gene sets related to the cell cycle pathway were upregulated in PDL. Thirteen upregulated and twenty downregulated genes were found through DEG analysis. Real-time PCR results confirmed that five upregulated genes (CC2D1B, CPNE3, OPHN1, TANGO2, and UAP-1) and six downregulated genes (MYOM2, PPM1F, PCDP1, ATP2A1, GPR171, and RP1-34H18.1-1) were consistent with RNA sequencing results. We suggest that, from among these eleven genes, two upregulated genes, CPNE3 and OPHN1, and one downregulated gene, PPM1F, play an important role in PDL regeneration in humans when orthodontic force is applied.
Central Resistance to Thyroid Hormones in Morbidly Obese Subjects Is Reversed after Bariatric Surgery-Induced Weight Loss
Authors: Juiz-Valiña Paula , Cordido María , Outeiriño-Blanco Elena , Pértega Sonia , Varela-Rodríguez Bárbara María , García-Brao María Jesús , Mena Enrique , Pena-Bello Lara , Sangiao-Alvarellos Susana , Cordido Fernando
Published: January 2020

Endocrine abnormalities are common in obesity, including altered thyroid function. The altered thyroid function of obesity may be due to a mild acquired resistance to the thyroid hormone. The aim of this study was to investigate the effect of weight loss after bariatric surgery (BS) on resistance to thyroid hormones in patients with extreme obesity compared with a control group. We performed an observational study evaluating patients with extreme obesity who underwent BS. We included 106 patients (83 women) and 38 controls (24 women). The primary endpoint was the thyrotroph thyroxine resistance index (TT4RI) and thyroid stimulating hormone (TSH) index (TSHRI). The parameters were studied before and after surgery. TSHRI and TT4RI were higher in the obese patients than in the control group. TT4RI and TSHI decreased significantly over time after surgery, with this decrease being associated with the excessive body mass index (BMI) loss and C-reactive protein (CRP). In extreme obesity, BS promotes a significant decrease in the increased TT4RI and TSHI. This decrease of TT4RI and TSHI is progressive over time after BS and significantly associated with excess BMI lost and CRP. Extreme obesity is characterized by a mild reversible central resistance to thyroid hormones.
Shoulder Muscle Strength and Neuromuscular Activation 2 Years after Reverse Shoulder Prosthesis—An Experimental Case Control Study
Authors: Rienmüller Anna , Maffiuletti Nicola A. , Schwyzer Hans-Kaspar , Eggspühler Andreas
Published: January 2020

Although reverse shoulder arthroplasty (RSA) has shown successful postoperative outcomes, little is known about compensatory activation patterns of remaining shoulder muscles following RSA. The purpose of this experimental case control series was to investigate shoulder muscle strength and neuromuscular activation of deltoid and teres minor muscles 2 years after RSA. Humerus lengthening, center-of-rotation medialization, maximal voluntary strength, and electromyographic (EMG) activity were compared between the operated and the non-operated side of 13 patients (mean age: 73 years). Shoulder muscle strength was significantly lower on the operated side for external rotation (−54%), internal rotation (−20%), and adduction (−13%). Agonist deltoid EMG activity was lower on the operated side for shoulder flexion, extension, and internal and external rotation (p < 0.05). Antagonist deltoid coactivation was higher on the operated side for external rotation (p < 0.001). Large correlation coefficients were observed between shoulder adductor strength asymmetry and both center-of-rotation medialization (r = −0.73) and humerus lengthening (r = 0.71). Shoulder abduction strength and neuromuscular activation were well preserved 2 years after RSA, while persistent strength and activation deficits were observed for shoulder adduction and internal and external rotation. Additional studies are required to elucidate shoulder neuromuscular activation patterns before and after RSA to support decision making for surgical, implant design, and rehabilitation choices.
Body-Mass-Index Associated Differences in Ortho- and Retronasal Olfactory Function and the Individual Significance of Olfaction in Health and Disease
Authors: Besser Gerold , Erlacher Brigitte , Aydinkoc-Tuzcu Kadriye , Liu David T. , Pablik Eleonore , Niebauer Verena , Koenighofer Martin , Renner Bertold , Mueller Christian A.
Published: January 2020

Odor (including flavor) perception plays a major role in dietary behavior. Orthonasal olfactory function (OOF) has been shown to decrease in obese subjects. Changes in retronasal olfactory function (ROF) after weight loss and in the individual significance of olfaction (ISO) in obesity are yet to be investigated. Firstly, 15 obese subjects were recruited in a pilot study and supported to conventionally lose weight. OOF (Sniffin’ Sticks) was measured at the beginning and after 5.6 ± 1.3 months. Eleven subjects re-visited but barely lost weight and no major changes in OOF were observed. Secondly, the body-mass-index (BMI), OOF, and ROF (Candy Smell Test, CST) were recorded in subjectively olfactory-healthy subjects (SOHSs) and additionally the ISO questionnaire was collected in patients with olfactory dysfunction (OD). BMI correlated significantly negatively with odor discrimination (p = 0.00004) in 74 SOHSs and negatively with CST (p < 0.0001) in 66 SOHSs. In 48 SOHSs, there was a gender difference in ISO scores (p = 0.034), but no significant correlation with BMI was found (p > 0.05). ISO scores were significantly higher in 52 OD patients in comparison to SOHSs (p = 0.0382). Not only OOF but also ROF may decline with higher BMI. ISO does not seem to alter with BMI, but olfaction becomes more important once it is consciously impaired.
Using Whole Breast Ultrasound Tomography to Improve Breast Cancer Risk Assessment: A Novel Risk Factor Based on the Quantitative Tissue Property of Sound Speed
Authors: Duric Neb , Sak Mark , Fan Shaoqi , Pfeiffer Ruth M. , Littrup Peter J. , Simon Michael S. , Gorski David H. , Ali Haythem , Purrington Kristen S. , Brem Rachel F. , Sherman Mark E. , Gierach Gretchen L.
Published: January 2020

Mammographic percent density (MPD) is an independent risk factor for developing breast cancer, but its inclusion in clinical risk models provides only modest improvements in individualized risk prediction, and MPD is not typically assessed in younger women because of ionizing radiation concerns. Previous studies have shown that tissue sound speed, derived from whole breast ultrasound tomography (UST), a non-ionizing modality, is a potential surrogate marker of breast density, but prior to this study, sound speed has not been directly linked to breast cancer risk. To that end, we explored the relation of sound speed and MPD with breast cancer risk in a case-control study, including 61 cases with recent breast cancer diagnoses and a comparison group of 165 women, frequency matched to cases on age, race, and menopausal status, and with a recent negative mammogram and no personal history of breast cancer. Multivariable odds ratios (ORs) and 95% confidence intervals (CIs) were estimated for the relation of quartiles of MPD and sound speed with breast cancer risk adjusted for matching factors. Elevated MPD was associated with increased breast cancer risk, although the trend did not reach statistical significance (OR per quartile = 1.27, 95% CI: 0.95, 1.70; ptrend = 0.10). In contrast, elevated sound speed was significantly associated with breast cancer risk in a dose–response fashion (OR per quartile = 1.83, 95% CI: 1.32, 2.54; ptrend = 0.0003). The OR trend for sound speed was statistically significantly different from that observed for MPD (p = 0.005). These findings suggest that whole breast sound speed may be more strongly associated with breast cancer risk than MPD and offer future opportunities for refining the magnitude and precision of risk associations in larger, population-based studies, including women younger than usual screening ages.
Loss-of-Function Variants in Cytoskeletal Genes Are Associated with Early-Onset Atrial Fibrillation
Authors: Vad Oliver Bundgaard , Paludan-Müller Christian , Ahlberg Gustav , Kalstø Silje Madeleine , Ghouse Jonas , Andreasen Laura , Haunsø Stig , Tveit Arnljot , Sajadieh Ahmad , Christophersen Ingrid Elisabeth , Svendsen Jesper Hastrup , Olesen Morten Salling
Published: January 2020

Atrial fibrillation (AF) is the most common cardiac arrhythmia, and it is associated with an increased risk of heart failure, stroke, dementia, and death. Recently, titin-truncating variants (TTNtv), which are predominantly associated with dilated cardiomyopathy (DCM), were associated with early-onset AF. Furthermore, genome-wide association studies (GWAS) associated AF with other structural genes. In this study, we investigated whether early-onset AF was associated with loss-of-function variants in DCM-associated genes encoding cytoskeletal proteins. Using targeted sequencing, we examined a cohort of 527 Scandinavian individuals with early-onset AF and a control group of individuals free of AF (n = 383). The patients had onset of AF before 50 years of age, normal echocardiogram, and no other cardiovascular disease at onset of AF. We identified six individuals with rare loss-of-function variants in three different genes (dystrophin (DMD), actin-associated LIM protein (PDLIM3), and fukutin (FKTN)), of which two variants were novel. Loss-of-function variants in cytoskeletal genes were significantly associated with early-onset AF when patients were compared with controls (p = 0.044). Using publicly available GWAS data, we performed genetic correlation analyses between AF and 13 other traits, e.g., showing genetic correlation between AF and non-ischemic cardiomyopathy (p = 0.0003). Our data suggest that rare loss-of-function variants in cytoskeletal genes previously associated with DCM may have a role in early-onset AF, perhaps through the development of an atrial cardiomyopathy.
A Virus Infected Your Laptop. Let’s Play an Escape Game
Authors: Queiruga-Dios Araceli , Santos Sánchez María Jesús , Queiruga Dios Marián , Gayoso Martínez Víctor , Hernández Encinas Ascensión
Published: January 2020

Have you ever thought what would happen if a ransomware infected your laptop? This type of virus kidnaps files and encrypts them, and the only way to recover the data is by paying in bitcoin or some other cryptocurrency. This situation is undoubtedly terrible. All of your work, projects, and personal files will not be available (unless you pay the ransom). The first time students watched a video that contextualizes this stressful situation, they thought that they had been attacked by a computer virus. Fortunately, the panic only lasted a few seconds. This is the way to start a game called breakout or escape room. The difference between these two words is that, in the first case, the goal is to open a padlocked box, while the objective of an escape room is to find the key that allows to get out of a room. Both games are similar, containing riddles, puzzles, problems and some clues that would help the players to find the solution. This study analyses the use of a breakout game for educational purposes, more specifically in a university context. The experiment conducted mixes game–based learning methodologies with engineering students learning Linear Algebra, Calculus or Cryptography, which has allowed us to obtain promising results about the usage of this methodology.
High Entropy Alloys: Ready to Set Sail?
Authors: Basu Indranil , De Hosson Jeff Th. M.
Published: January 2020

Over the past decade, high entropy alloys (HEAs) have transcended the frontiers of material development in terms of their unprecedented structural and functional properties compared to their counterpart conventional alloys. The possibility to explore a vast compositional space further renders this area of research extremely promising in the near future for discovering society-changing materials. The introduction of HEAs has also brought forth a paradigm shift in the existing knowledge about material design and development. It is in this regard that a fundamental understanding of the metal physics of these alloys is critical in propelling mechanism-based HEA design. The current paper highlights some of the critical viewpoints that need greater attention in the future with respect to designing mechanically and functionally advanced materials. In particular, the interplay of large compositional gradients and defect topologies in these alloys and their corresponding impact on overall mechanical response are highlighted. From the point of view of functional response, such chemistry vis-à-vis topology correlations are extended to novel class of nano-porous HEAs that beat thermal coarsening effects despite a high surface to volume ratio owing to retarded diffusion kinetics. Recommendations on material design with regards to their potential use in diverse applications such as energy storage, actuators, and as piezoelectrics are additionally considered.
Integrated Numerical-Experimental Assessment of the Effect of the AZ31B Anisotropic Behaviour in Extended-Surface Treatments by Laser Shock Processing
Authors: Angulo Ignacio , Cordovilla Francisco , García-Beltrán Ángel , Porro Juan A. , Díaz Marcos , Ocaña José L.
Published: January 2020

In recent years, an increasing interest in designing magnesium biomedical implants has been presented due to its biocompatibility, and great effort has been employed in characterizing it experimentally. However, its complex anisotropic behaviour, which is observed in rolled alloys, leads to a lack of reliable numerical simulation results concerning residual stress predictions. In this paper, a new model is proposed to focus on anisotropic material hardening behaviour in Mg base (in particular AZ31B as a representative alloy) materials, in which the particular stress cycle involved in Laser Shock Processing (LSP) treatments is considered. Numerical predictions in high extended coverage areas obtained by means of the implemented model are presented, showing that the realistic material’s complex anisotropic behaviour can be appropriately computed and—much more importantly—it shows a particular non-conventional behaviour regarding extended areas processing strategies.
Metabolomics as an Emerging Tool for the Study of Plant–Pathogen Interactions
Authors: Castro-Moretti Fernanda R. , Gentzel Irene N. , Mackey David , Alonso Ana P.
Published: January 2020

Plants defend themselves from most microbial attacks via mechanisms including cell wall fortification, production of antimicrobial compounds, and generation of reactive oxygen species. Successful pathogens overcome these host defenses, as well as obtain nutrients from the host. Perturbations of plant metabolism play a central role in determining the outcome of attempted infections. Metabolomic analyses, for example between healthy, newly infected and diseased or resistant plants, have the potential to reveal perturbations to signaling or output pathways with key roles in determining the outcome of a plant–microbe interaction. However, application of this -omic and its tools in plant pathology studies is lagging relative to genomic and transcriptomic methods. Thus, it is imperative to bring the power of metabolomics to bear on the study of plant resistance/susceptibility. This review discusses metabolomics studies that link changes in primary or specialized metabolism to the defense responses of plants against bacterial, fungal, nematode, and viral pathogens. Also examined are cases where metabolomics unveils virulence mechanisms used by pathogens. Finally, how integrating metabolomics with other -omics can advance plant pathology research is discussed.
From Clay Minerals to Al2O3 Nanoparticles: Synthesis and Colloidal Stabilization for Optoelectronic Applications
Authors: Botero Yesica L. , López-Rendón Jorge Eliécer , Ramírez Daniel , Zapata Dioni Mabel , Jaramillo Franklin
Published: January 2020

This research was performed to obtain high-value products from clay materials. High-grade nanometric delta-alumina (δ-Al2O3) was obtained from the modification of clay-based minerals, which could be potentially applied in the form of thin film for novel optoelectronic applications. The selective recovery process of alumina from clay materials presents an important advantage regarding the complete removal of other starting constituents such as silica, iron, titanium, alkali, and alkaline earth metals. To accomplish the selective removal of different species, an acid leaching route was used to extract the aluminum, then the iron impurities were eliminated by alkaline precipitation. The solution was acidized to precipitate the aluminum as aluminum chloride hexahydrate. Finally, the aluminum chloride hexahydrate was calcinated to obtain nano-delta-alumina with purity of over 98.5% Al2O3. The dominating crystalline phase was delta–gamma alumina (δ-phase and γ-phase), with a particle size of <140 nm. Then, these nanoparticles (NPs) were prepared as a stable colloidal dispersion to form a mesoporous layer employing the spin-coating technique. Initially, the synthesized alumina was characterized by atomic force microscopy (AFM) and TEM to determine the particle size and its morphology, whereas the colloidal dispersion was analyzed by rheological measurements. Finally, the findings of this investigation made it possible to get thin films with good porosity, which can be used in optoelectronic applications, specifically in perovskite solar cells.