bluuebunny/crossref_metadata_2025 huggingface dataset:
Two-Dimensional Audio Compression Method Using Video Coding Schemes
Authors: Kim Seonjae , Jun Dongsan , Kim Byung-Gyu , Beack Seungkwon , Lee Misuk , Lee Taejin
Published: May 2021

As video compression is one of the core technologies that enables seamless media streaming within the available network bandwidth, it is crucial to employ media codecs to support powerful coding performance and higher visual quality. Versatile Video Coding (VVC) is the latest video coding standard developed by the Joint Video Experts Team (JVET) that can compress original data hundreds of times in the image or video; the latest audio coding standard, Unified Speech and Audio Coding (USAC), achieves a compression rate of about 20 times for audio or speech data. In this paper, we propose a pre-processing method to generate a two-dimensional (2D) audio signal as an input of a VVC encoder, and investigate the applicability to 2D audio compression using the video coding scheme. To evaluate the coding performance, we measure both signal-to-noise ratio (SNR) and bits per sample (bps). The experimental result shows the possibility of researching 2D audio encoding using video coding schemes.
The EpiDiverse Plant Epigenome-Wide Association Studies (EWAS) Pipeline
Authors: Can Sultan Nilay , Nunn Adam , Galanti Dario , Langenberger David , Becker Claude , Volmer Katharina , Heer Katrin , Opgenoorth Lars , Fernandez-Pozo Noe , Rensing Stefan A.
Published: May 2021

Bisulfite sequencing is a widely used technique for determining DNA methylation and its relationship with epigenetics, genetics, and environmental parameters. Various techniques were implemented for epigenome-wide association studies (EWAS) to reveal meaningful associations; however, there are only very few plant studies available to date. Here, we developed the EpiDiverse EWAS pipeline and tested it using two plant datasets, from P. abies (Norway spruce) and Q. lobata (valley oak). Hence, we present an EWAS implementation tested for non-model plant species and describe its use.
Stakeholders’ Perception of the Impact of the Declaration of New Protected Areas on the Development of the Regions Concerned, Case Study: Czech Republic
Authors: Schneider Jiří , Ruda Aleš , Blahová Martina
Published: May 2021

Floodplain forests at the confluence of the rivers Dyje and Morava (in the southeastern tip of the Czech Republic) are completely unique ecosystems in terms of area and ecology. For many years, there has been an effort by the state’s nature protection officials to declare the area as a Protected Landscape Area. This effort is met by the resistance of foresters and other local stakeholders. The study focuses on the identification of stakeholders’ comments and objections to the planned declaration of the Soutok PLA and the comparison between the objections raised and the attitudes of stakeholders from existing PLAs. Using the content analysis of 247 paper documents, the first part of the study determines the negative arguments that are subsequently verified in the second part on the basis of 17 semistructured standardized interviews and interview surveys of 200 respondents. The analysis of the interviews and surveys was based on the grounded theory method. The theoretical sampling and snowball techniques were used to recruit the respondents. The interviews and surveys showed that most concerns over restrictions established by the conservation status are unnecessary since experience showed that they are either not registered or not established by the PLA status, and their application is provided by other legislative standards.
Continuity Result on the Order of a Nonlinear Fractional Pseudo-Parabolic Equation with Caputo Derivative
Authors: Binh Ho Duy , Hoang Luc Nguyen , Baleanu Dumitru , Van Ho Thi Kim
Published: May 2021

In this paper, we consider a problem of continuity fractional-order for pseudo-parabolic equations with the fractional derivative of Caputo. Here, we investigate the stability of the problem with respect to derivative parameters and initial data. We also show that uω′→uω in an appropriate sense as ω′→ω, where ω is the fractional order. Moreover, to test the continuity fractional-order, we present several numerical examples to illustrate this property.
Influence of Age on Skeletal Muscle Hypertrophy and Atrophy Signaling: Established Paradigms and Unexpected Links
Authors: Lee Eun-Joo , Neppl Ronald L.
Published: May 2021

Skeletal muscle atrophy in an inevitable occurrence with advancing age, and a consequence of disease including cancer. Muscle atrophy in the elderly is managed by a regimen of resistance exercise and increased protein intake. Understanding the signaling that regulates muscle mass may identify potential therapeutic targets for the prevention and reversal of muscle atrophy in metabolic and neuromuscular diseases. This review covers the major anabolic and catabolic pathways that regulate skeletal muscle mass, with a focus on recent progress and potential new players.
Novel PITX2 Homeodomain-Contained Mutations from ATRIAL Fibrillation Patients Deteriorate Calcium Homeostasis
Authors: Herraiz-Martínez Adela , Tarifa Carmen , Lozano-Velasco Estefanía , Jiménez-Sábado Verónica , Casabella Sergi , Hernández-Torres Francisco , Daimi Houria , Vázquez Ruiz de Castroviejo Eduardo , Delpón Eva , Caballero Ricardo , Aránega Amelia , Franco Diego , Hove-Madsen Leif
Published: May 2021

Atrial fibrillation (AF) is the most common cardiac arrhythmia in the human population, with an estimated incidence of 1–2% in young adults but increasing to more than 10% in 80+ years patients. Pituitary Homeobox 2, Paired Like Homeodomain 2 (PITX2c) loss-of-function in mice revealed that this homeodomain (HD)-containing transcription factor plays a pivotal role in atrial electrophysiology and calcium homeostasis and point to PITX2 as a candidate gene for AF. To address this issue, we recruited 31 AF patients for genetic analyses of both the known risk alleles and PITX2c open reading frame (ORF) re-sequencing. We found two-point mutations in the homedomain of PITX2 and three other variants in the 5’untranslated region. A 65 years old male patient without 4q25 risk variants but with recurrent AF displayed two distinct HD-mutations, NM_000325.5:c.309G>C (Gln103His) and NM_000325.5:c.370G>A (Glu124Lys), which both resulted in a change within a highly conserved amino acid position. To address the functional impact of the PITX2 HD mutations, we generated plasmid constructs with mutated version of each nucleotide variant (MD4 and MD5, respectively) as well as a dominant negative control construct in which the PITX2 HD was lacking (DN). Functional analyses demonstrated PITX2c MD4 and PITX2c MD5 decreased Nppa-luciferase transactivation by 50% and 40%, respectively, similar to the PITX2c DN (50%), while Shox2 promoter repression was also impaired. Co-transactivation with other cardiac-enriched co-factors, such as Gata4 and Nkx2.5, was similarly impaired, further supporting the pivotal role of these mutations for correct PITX2c function. Furthermore, when expressed in HL1 cardiomyocyte cultures, the PITX2 mutants impaired endogenous expression of calcium regulatory proteins and induced alterations in sarcoplasmic reticulum (SR) calcium accumulation. This favored alternating and irregular calcium transient amplitudes, causing deterioration of the beat-to-beat stability upon elevation of the stimulation frequency. Overall this data demonstrate that these novel PITX2c HD-mutations might be causative of atrial fibrillation in the carrier.
Vicarious Traumatization Questionnaire: Psychometric Properties Using Rasch Model and Structural Equation Modeling
Authors: Norhayati Mohd Noor , Mofreh Samah Ali Mohsen , Azman Yacob Mohd
Published: May 2021

Frontline healthcare providers are exposed to indirect trauma through dealing with traumatized patients. This puts them at risk of vicarious traumatization. In response to the COVID-19 pandemic, this study seeks to establish the psychometric properties of the Malay version of the Vicarious Traumatization Questionnaire among healthcare providers. A cross-sectional study was conducted. The translated Malay version of the Vicarious Traumatization Questionnaire was completed by 352 healthcare providers in Kelantan, Malaysia. The data was entered using IBM SPSS Statistics version 26.0 (SPSS Inc., Chicago, IL, USA, 2019), and descriptive analysis was performed. The psychometric properties of the scale were assessed in two phases. The Rasch model to assess the validity and reliability was performed using Winsteps version 3.72.3. The confirmatory factor analysis using the structural equation modeling was performed using AMOS version 23.0. The Rasch analysis showed that the 38 items, in two constructs, had high item reliability and item separation at 0.97 and item separation at 5.36, respectively, while good person reliability and person separation were at 0.95 and 4.58, respectively. The correlations of all persons and items are greater than 0.20. There are no misfitting or overfitting items in the outfit MNSQ. There are four items that are challenging in answering the scale. The final model of the confirmatory factor analysis shows two constructs with 38 items demonstrating acceptable factor loadings, domain to domain correlation, and best fit (Chi-squared/degree of freedom = 4.73; Tucker-Lewis index = 0.94; comparative fit index = 0.94; and root mean square error of approximation = 0.10). Composite reliability and average variance extracted of the domains were higher than 0.7 and 0.5, respectively. The Vicarious Traumatization Questionnaire tested among healthcare providers has been shown to valid and reliable to assess vicarious traumatization.
Trait and State: Interoceptive Accuracy during Anticipation of Public Speaking in Junior Secondary Shy Students from an Eastern Province of China
Authors: Wu Jianfen , Li Hui , Wu Yunpeng
Published: May 2021

This study investigated the interoceptive accuracy (IAc) of shy adolescents during anticipation of public speaking with a 2 × 2 factorial design. Altogether, 637 junior secondary students in an eastern province of China were sampled and screened with the Chinese version of Cheek and Buss shyness scale. The top 27% of students were considered the shy group (n = 30, 16 girls, Mage = 13.03, SD = 0.67), whereas the bottom 27% were labelled the non-shy group (n = 31, 16 girls, Mage = 13.16, SD = 0.86). The two groups of participants estimated their heart rates during specified intervals using a mental tracking paradigm in two conditions (baseline vs. anticipation), while their actual heart rates were simultaneously measured. The results indicated that: (1) the shy adolescents were more accurate in estimating their actual heart rate than non-shy adolescents; and (2) both shy and non-shy adolescents exhibit enhanced IAc in anticipation conditions when compared with baseline conditions. Implications of the higher IAc of shy adolescents and the state feature of IAc are discussed.
The Role of Social Stress in the Development of Inhibitory Control Deficit: A Systematic Review in Preclinical Models
Authors: Sánchez-Salvador Lucía , Prados-Pardo Ángeles , Martín-González Elena , Olmedo-Córdoba Manuela , Mora Santiago , Moreno Margarita
Published: May 2021

Inhibitory control deficit and impulsivity and compulsivity behaviours are present in different psychopathological disorders such as addiction, obsessive-compulsive disorders and schizophrenia, among others. Social relationships in humans and animals are governed by social organization rules, which modulate inhibitory control and coping strategies against stress. Social stress is associated with compulsive alcohol and drug use, pointing towards a determining factor in an increased vulnerability to inhibitory control deficit. The goal of the present review is to assess the implication of social stress and dominance on the vulnerability to develop impulsive and/or compulsive spectrum disorders, with the aid of the information provided by animal models. A systematic search strategy was carried out on the PubMed and Web of Science databases, and the most relevant information was structured in the text and tables. A total of 34 studies were recruited in the qualitative synthesis. The results show the role of social stress and dominance in increased drug and alcohol use, aggressive and impulsive behaviour. Moreover, the revised studies support the role of Dopaminergic (DA) activity and the alterations in the dopaminergic D1/D2 receptors as key factors in the development of inhibitory control deficit by social stress.
Vegetarian and Vegan Diet in Fibromyalgia: A Systematic Review
Authors: Nadal-Nicolás Yolanda , Miralles-Amorós Laura , Martínez-Olcina María , Sánchez-Ortega María , Mora Juan , Martínez-Rodríguez Alejandro
Published: May 2021

Fibromyalgia (FM) is a chronic non-degenerative disease characterized by the presence of multiple symptoms such as chronic pain, which negatively influence the quality of life of sufferers, most of whom are women. Currently, there is no effective treatment to limit the impact of these symptoms. The aim of this research is to review the scientific evidence on the effect of following a vegetarian or vegan diet on fibromyalgia patients. A systematic review included the original articles that answered the research question. These articles were in 2021 in the PubMed, Web of Science and Scopus databases. The research used the PRISMA (preferred reporting items for systematic reviews and meta-analyses) guidelines. No time restriction was applied, and grey literature was not included. The evaluation of the methodological quality of the articles was carried out using the following different scales: STROBE (strengthening the reporting of observational studies in epidemiology), PEDro (Physiotherapy Evidence Database), and MMAT (Mixed Methods Appraisal Tool) scales. A total of 88 studies were analyzed, of which 6 investigations were included in this systematic review (n = 4 clinical trials and n = 2 cohort studies). These investigations show significant improvements in biochemical parameters, quality of life, quality of sleep, pain at rest and general health status when following mainly plant-based dietary patterns. In conclusion, these findings are promising but interpretation of the findings is limited due to the methodological quality of the studies. Well-designed clinical trials are needed to consolidate these dietary recommendations in FM patients.
Combatting Visual Fake News with a Professional Fact-Checking Tool in Education in France, Romania, Spain and Sweden
Authors: Nygren Thomas , Guath Mona , Axelsson Carl-Anton Werner , Frau-Meigs Divina
Published: May 2021

Educational and technical resources are regarded as central in combating disinformation and safeguarding democracy in an era of ‘fake news’. In this study, we investigated whether a professional fact-checking tool could be utilised in curricular activity to make pupils more skilled in determining the credibility of digital news and to inspire them to use digital tools to further their transliteracy and technocognition. In addition, we explored how pupils’ performance and attitudes regarding digital news and tools varied across four countries (France, Romania, Spain, and Sweden). Our findings showed that a two-hour intervention had a statistically significant impact on teenagers’ abilities to determine the credibility of fake images and videos. We also found that the intervention inspired pupils to use digital tools in information credibility assessments. Importantly, the intervention did not make pupils more sceptical of credible news. The impact of the intervention was greater in Romania and Spain than among pupils in Sweden and France. The greater impact in these two countries, we argue, is due to cultural context and the fact that pupils in Romania and Spain learned to focus less on ’gut feelings’, increased their use of digital tools, and had a more positive attitude toward the use of the fact-checking tool than pupils in Sweden and France.
Sterile Insect Technique Programme against Mediterranean Fruit Fly in the Valencian Community (Spain)
Authors: Plá Ignacio , García de Oteyza Jaime , Tur Carlos , Martínez Miguel Ángel , Laurín María Carmen , Alonso Ester , Martínez Marta , Martín Ángel , Sanchis Román , Navarro María Carmen , Navarro María Teresa , Argilés Rafael , Briasco Marta , Dembilio Óscar , Dalmau Vicente
Published: May 2021

The Mediterranean fruit fly, Ceratitis capitata (Wied.), is an endemic pest in fruit-growing areas of the Spanish Mediterranean coast. In the Valencian Community, it represents a serious problem in the cultivation of citrus and numerous species of fruit, such as peach, cherry, apricot, persimmon, etc. For over 50 years, the Department of Agriculture of Valencia has led, promoted, and carried out a C. capitata control programme to protect crops, especially citrus fruits, because this community is the largest national producer and the leading region for fresh citrus exports in the world. Traditionally, pest control has been based on the use of insecticides. However, a reduction of more than 90% of a target wild population was achieved in the frame of a pilot integrated pest management (IPM) project based on the sterile insect technique (SIT), which was implemented from 2003 to 2006. Based on this successful result, in 2007 the Department of Agriculture of Valencia initiated an area-wide integrated pest management (AW-IPM) programme for the suppression of C. capitata, using the SIT as the primary control method. Complementary activities are implemented periodically in hotspots and during different time periods depending on the pest population dynamics. As a result, there has been a reduction of more than 90% in the use of insecticides by aerial means to control C. capitata, as well as a growth trend in exports of citrus and fresh fruits from the Valencian Community in recent years. This paper provides a historical review of the Valencian programme and briefly describes how technological innovations and decision-making tools have contributed to programme efficiency.
Niche Modeling May Explain the Historical Population Failure of Phytoseiulus persimilis in Taiwan: Implications of Biocontrol Strategies
Authors: Liao Jhih-Rong , Ho Chyi-Chen , Chiu Ming-Chih , Ko Chiung-Cheng
Published: May 2021

Biological control commonly involves the commercialization and introduction of natural enemies. Phytoseiulus persimilis Athias-Henriot, a mite species widely used in the control of spider mites, was imported to Taiwan in the 1990s and was mass-reared and released into the field. However, none have been observed in comprehensive surveys of phytoseiid mites for over 30 years. In this study, the distribution of P. persimilis in Taiwan was predicted, and environmental variables that affect its distribution were analyzed. The mountainous region of southcentral Taiwan was determined to be suitable for the establishment of this species, whereas the four sites at which it was released in the 1990s, particularly those in southwestern Taiwan, exhibited low suitability. Notably, the minimum temperature of the coldest month was identified as a crucial limiting factor affecting the distribution of P. persimilis, indicating that a Mediterranean climate is more suitable for this species. To the best of our knowledge, this study is the first to predict the suitable distribution of exotic predatory mites in a biological control program. The present findings serve as a pivotal assessment framework for the commercialization and foreign introduction of natural enemies.
Sarcoidosis-Like Cancer-Associated Granulomatosis: Characteristics and a Case-Control Comparison with Sarcoidosis
Authors: Pastré Jean , Bouvry Diane , Juvin Karine , Benattia Amira , Annesi-Maesano Isabella , Valeyre Dominique , Nunes Hilario , Israël-Biet Dominique
Published: May 2021

(1) Background: Systemic granulomatosis developed in a context of malignancy has already been reported. Our objective was to describe the clinical, radiological, functional, biological, and evolutive characteristics of sarcoidosis-like cancer-associated granulomatosis (SLCAG) and to compare them to those of sarcoidosis. (2) Methods: 38 patients with a biopsy-proven SLCAG developed after a diagnostic of malignancy were included. The control group consisted of sarcoidosis patients matched for age, sex, and radiologic stage. Clinical, biological, physiological, radiological, and outcome data were collected. (3) Results: The mean age of SLCAG patients was 51 ± 14 years. They were diagnosed within 15 ± 14 months of the cancer diagnosis (breast cancer most frequently). All SLCAG patients presented a thoracic involvement, extrathoracic locations were observed in 32% of subjects. SLCAG was more often asymptomatic than sarcoidosis (p < 0.0001). During follow-up, systemic treatment was less often required in SLCAG than in sarcoidosis (58% vs. 32%, p = 0.04 respectively) and SLCAG were characterized by a significantly less severe progression profile according to the Sarcoid Clinical Activity Classification, with a complete recovery more frequent at 5 years (p = 0.03). (4) Conclusion: This case-control study shows that SLCAG differs from sarcoidosis with a significantly more benign course. These results might argue for true differences in the physiopathology, which remain to be elucidated.
Bone Effect and Safety of One-Year Denosumab Therapy in a Cohort of Renal Transplanted Patients: An Observational Monocentric Study
Authors: Alfieri Carlo , Binda Valentina , Malvica Silvia , Cresseri Donata , Campise Mariarosaria , Gandolfo Maria Teresa , Regalia Anna , Mattinzoli Deborah , Armelloni Silvia , Favi Evaldo , Molinari Paolo , Messa Piergiorgio
Published: May 2021

In 32-kidney transplanted patients (KTxps), the safety and the effects on BMD and mineral metabolism (MM) of one-year treatment with denosumab (DB) were studied. Femoral and vertebral BMD and T-score, FRAX score and vertebral fractures (sVF) before (T0) and after 12 months (T12) of treatment were measured. MM, renal parameters, hypocalcemic episodes (HpCa), urinary tract infections (UTI), major graft and KTxps outcomes were monitored. The cohort was composed mainly of females, n = 21. We had 29 KTxps on steroid therapy and 22 KTxps on vitamin D supplementation. At T0, 25 and 7 KTxps had femoral osteoporosis (F-OPS) and osteopenia (F-OPS), respectively. Twenty-three and six KTxps had vertebral osteoporosis (V-OPS) and osteopenia (V-OPS), respectively. Seventeen KTxps had sVF. At T12, T-score increased at femoral and vertebral sites (p = 0.05, p = 0.008). The prevalence of F-OPS and V-OPS reduced from 78% to 69% and from 72% to 50%, respectively. Twenty-five KTxps ameliorated FRAX score and two KTxps had novel sVF. At T12, a slight reduction of Ca was present, without HpCa. Four KTxps had UTI. No graft rejections, loss of graft or deaths were reported. Our preliminary results show a good efficacy and safety of DB in KTxps. Longer and randomized studies involving more KTxps might elucidate the possible primary role of DB in the treatment of bone disorders in KTxps.
Cardiac Imaging in Anderson-Fabry Disease: Past, Present and Future
Authors: Esposito Roberta , Santoro Ciro , Mandoli Giulia Elena , Cuomo Vittoria , Sorrentino Regina , La Mura Lucia , Pastore Maria Concetta , Bandera Francesco , D’Ascenzi Flavio , Malagoli Alessandro , Benfari Giovanni , D’Andrea Antonello , Cameli Matteo
Published: May 2021

Anderson-Fabrydisease is an X-linked lysosomal storage disorder caused by a deficiency in the lysosomal enzyme α-galactosidase A. This results in pathological accumulation of glycosphingolipids in several tissues and multi-organ progressive dysfunction. The typical clinical phenotype of Anderson-Fabry cardiomyopathy is progressive hypertrophic cardiomyopathy associated with rhythm and conduction disturbances. Cardiac imaging plays a key role in the evaluation and management of Anderson-Fabry disease patients. The present review highlights the value and perspectives of standard and advanced cardiovascular imaging in Anderson-Fabry disease.
Cerebral Vasoreactivity Evaluated by Transcranial Color Doppler and Breath-Holding Test in Patients after SARS-CoV-2 Infection
Authors: Marcic Marino , Marcic Ljiljana , Marcic Barbara , Capkun Vesna , Vukojevic Katarina
Published: May 2021

From the beginning of the SARS-CoV-2 virus pandemic, it was clear that the virus is highly neurotrophic. Neurological manifestations can range from nonspecific symptoms such as dizziness, headaches and olfactory disturbances to severe forms of neurological dysfunction. Some neurological complication can occur even after mild forms of respiratory disease. This study’s aims were to assess cerebrovascular reactivity in patients with nonspecific neurological symptoms after SARS-CoV-2 infection. A total of 25 patients, aged 33–62 years, who had nonspecific neurological symptoms after SARS-CoV-2 infection, as well as 25 healthy participants in the control group, were assessed for cerebrovascular reactivity according to transcranial color Doppler (TCCD) which we combined with a breath-holding test (BHT). In subjects after SARS-CoV-2 infection, there were statistically significantly lower flow velocities through the middle cerebral artery at rest period, lower maximum velocities at the end of the breath-holding period and lower breath holding index (BHI) in relation to the control group. Changes in cerebral artery flow rate velocities indicate poor cerebral vasoreactivity in the group after SARS-CoV-2 infection in regard to the control group and suggest vascular endothelial damage by the SARS-CoV-2 virus.
Special Attention to Physical Activity in Breast Cancer Patients during the First Wave of COVID-19 Pandemic in Italy: The DianaWeb Cohort
Authors: Natalucci Valentina , Villarini Milena , Emili Rita , Acito Mattia , Vallorani Luciana , Barbieri Elena , Villarini Anna
Published: May 2021

Recent evidence highlights that physical activity (PA) is associated with decreased recurrence risk, improved survival and quality of life for breast cancer (BC) patients. Our study aimed to explore patterns of increased/decreased PA, and sedentary behaviors among BC women of the DianaWeb cohort during the first wave of COVID-19 pandemic, and examined the association with residential locations, work changes, different modality used to increase PA, and quality of life. The study analyzed the questionnaires completed by the 781 BC women (age 54.68 ± 8.75 years on both December 2019 and June 2020. Results showed a decrease of 22%, 57%, and 26% for walking activity, vigorous activity, and total PA, respectively. Sitting/lying time increased up to 54.2% of the subjects recruited. High quality of life was associated with lower odds of being sedentary (p = 0.003). Our findings suggest that innovative health management fostering compliance with current guidelines for PA and active behavior should be implemented, especially in unpredictable emergency conditions.
User VR Experience and Motivation Study in an Immersive 3D Geovisualization Environment Using a Game Engine for Landscape Design Teaching
Authors: Carbonell-Carrera Carlos , Saorin Jose Luis , Melián Díaz Dámari
Published: May 2021

Realistic 3D geovisualization is necessary to facilitate the perception of a landscape designer in relation to the environment, which is a determining factor in decision-making in landscape planning and management. In the field of landscape design teaching learning environments, game engines can offer an immersive 3D geovisualization mode through Virtual Reality technology, which, in addition, can be motivating for the student. Game engines allow designing the scenarios where videogames take place, but game engines can also be used for geovisualization tasks in landscape design teaching environments. In this article, we present the landscape workshop, using a Unity 3D game engine. Twenty-five architect students performed landscape design tasks and worked with an interactive 3D geovisualization low-immersive desktop screen environment. The perception of the 3D environment during geovisualization was analyzed through the Questionnaire on User eXperience in Immersive Virtual Environments, and the motivational factor with the Intrinsic Motivation Inventory. Results showed a high perception of the 3D environment during geovisualization in the nine subcategories (sense of presence, engagement, immersion, flow, usability, emotion, judgment, experience consequence, and technology adoption) analyzed. The game engine-based teaching approach carried out has been motivating for students, with values over 5 (in a 1–7 Likert scale) in the five subscales considered.
Protein Extractions from Amphistegina lessonii: Protocol Development and Optimization
Authors: Betti Michele , Ciacci Caterina , Abramovich Sigal , Frontalini Fabrizio
Published: May 2021

Proteins are essential to life, and the evaluation of their content, identification, and modification represents a fundamental assay in biochemistry research. Different analytical techniques and protocols have been specifically designed but have rarely been compared. Here, we test and compare a variety of methodologies and treatments for the quantification of proteins in Amphistegina lessonii, a larger symbiont-bearing benthic foraminiferal species. These analyses specifically include (a) lysis buffer (homemade vs. RIPA), (b) protein assays (Lowry, BCA, and Bradford), (c) ultrasonic bath treatment, and (d) protein staining (silver staining vs. Coomassie blue). On the basis of the comparative outcome, we suggest using the homemade lysis buffer, Lowry or BCA assays, ultrasonic bath treatment, and silver stain to maximize the extraction and characterization of protein for A. lessonii. This protocol might be suitable and extended to other benthic foraminiferal species, including the smaller ones.
Loose Ends in the Cortinarius Phylogeny: Five New Myxotelamonoid Species Indicate a High Diversity of These Ectomycorrhizal Fungi with South American Nothofagaceae
Authors: Salgado Salomón María Eugenia , Barroetaveña Carolina , Niskanen Tuula , Liimatainen Kare , Smith Matthew E. , Peintner Ursula
Published: May 2021

This paper is a contribution to the current knowledge of taxonomy, ecology and distribution of South American Cortinarius (Pers.) Gray. Cortinarius is among the most widely distributed and species-rich basidiomycete genera occurring with South American Nothofagaceae and species are found in many distinct habitats, including shrublands and forests. Due to their ectomycorrhizal role, Cortinarius species are critical for nutrient cycling in forests, especially at higher latitudes. Some species have also been reported as edible fungi with high nutritional quality. Our aim is to unravel the taxonomy of selected Cortinarius belonging to phlegmacioid and myxotelamonioid species based on morphological and molecular data. After widely sampling Cortinarius specimens in Patagonian Nothofagaceae forests and comparing them to reference collections (including holotypes), we propose five new species of Cortinarius in this work. Phylogenetic analyses of concatenated rDNA ITS-LSU and RPB1 sequences failed to place these new species into known Cortinarius sections or lineages. These findings highlight our knowledge gaps regarding the fungal diversity of South American Nothofagaceae forests. Due to the high diversity of endemic Patagonian taxa, it is clear that the South American Cortinarius diversity needs to be discovered and described in order to understand the evolutionary history of Cortinarius on a global scale.
Herbal Extract from Codonopsis pilosula (Franch.) Nannf. Enhances Cardiogenic Differentiation and Improves the Function of Infarcted Rat Hearts
Authors: Wang Jieh-Neng , Kan Chung-Dann , Lee Lain-Tze , Huang Lynn L. H. , Hsiao Ya-Li , Chang Allen H. , Liu Wanchun , Lin Cheng , Lin Chou-Wen
Published: May 2021

Background: The roots of Codonopsis pilosula (Franch.) Nannf. have been used in traditional Chinese medicine for treating cardiovascular disease. In the current study, we aimed to discover herbal extracts from C. pilosula that are capable of improving cardiac function of infarcted hearts to develop a potential therapeutic approach. Methods: A mouse embryonic stem (ES) cell-based model with an enhanced green fluorescent protein (eGFP) reporter driven by a cardiomyocyte-specific promoter, the α-myosin heavy chain, was constructed to evaluate the cardiogenic activity of herbal extracts. Then, herbal extracts from C. pilosula with cardiogenic activity based on an increase in eGFP expression during ES cell differentiation were further tested in a rat myocardial infarction model with left anterior descending artery (LAD) ligation. Cardiac function assessments were performed using echocardiography, 1, 3, and 6 weeks post LAD ligation. Results: The herbal extract 417W from C. pilosula was capable of enhancing cardiogenic differentiation in mouse ES cells in vitro. Echocardiography results in the LAD-ligated rat model revealed significant improvements in the infarcted hearts at least 6 weeks after 417W treatment that were determined based on left ventricle fractional shortening (FS), fractional area contraction (FAC), and ejection fraction (EF). Conclusions: The herbal extract 417W can enhance the cardiogenic differentiation of ES cells and improve the cardiac function of infarcted hearts.
The Impact of the NLRP3 Pathway in the Pathogenesis of Non-Alcoholic Fatty Liver Disease and Alcohol-Related Liver Disease
Authors: Sheriff Lozan , Lalor Patricia F.
Published: May 2021

The presence of hepatic steatosis and inflammation is increasingly associated with both metabolic and alcohol-related liver conditions. Both are on the increase globally and, apart from liver transplantation, there are no licensed therapies that target the full complement of disease features. The presence of some shared pathogenic mechanisms and histological features in NAFLD and ALD suggests that it may be possible to develop markers for prognostication or staging, or indeed new therapeutic tools to treat both conditions. One such example of an approach exists in the form of the NACHT-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome pathway. Activation of the NLRP3 inflammasome results in hepatocyte pyroptosis, persistence, and amplification of liver inflammation and activation of profibrogenic signaling cascades. Thus, targeting elements of the pathway in NAFLD and ALD may provide a tractable route to pharmacological therapy. In this review, we summarize the contribution of this inflammasome to disease and review the current options for therapy.
The Value of Ultrasound in the Evaluation of the Integrity of Silicone Breast Implants
Authors: Rukanskienė Dalia , Bytautaitė Greta , Česnauskaitė Agnė , Pilipaitytė Loreta , Aštrauskas Tautrimas , Jonaitienė Eglė
Published: May 2021

Background and Objective: Breast implant surgery for cosmetic purposes is the most popular plastic surgery and it has been performed for over 100 years. Rupture of silicone gel-filled breast implants usually is asymptomatic and is one of the more dangerous complications due to free silicone migration. The aim of our study was to evaluate the diagnostic value of ultrasound (US) in the evaluation of the integrity of silicone breast implants and identify the main sign of intact and ruptured breast implants. Patients and Methods: In this retrospective study, the medical documentation of women who underwent breast implant surgery and US checkups at Tautrimas Aštrauskas Clinic in Kaunas, Lithuania, during 2015–2020 was analyzed. The patients were divided into two groups: patients with intact and ruptured breast implants. The accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) as well as the signs of implant integrity of US examination were evaluated. Results: In this study, 76 women with bilateral breast implants (n = 152) were reviewed. On a US examination, ruptured implants were found in 41.1% (n = 61) of the cases; of them, 78.7% (n = 48) of the cases had ≥2 US signs of a ruptured implant, and in all these cases, implant rupture was confirmed at surgery. Overall, one US sign of a ruptured implant was found in 21.3% (n = 13) of the cases. Of them, inhomogeneous content in all cases (n = 3) was found in the intact implant group, and an abnormal implant shell was documented more often in the ruptured implant group, not intact one (n = 9, 90% vs. n = 1, 10%). US had a diagnostic accuracy of 94.7%, sensitivity of 98.3%, specificity of 89.2%, PPV of 93.4%, and NPV of 97.1% in the evaluation of implant integrity. Conclusions: Our results show that US is a very reliable alternative in evaluating breast implant integrity and could be the investigation of choice for implant rupture, while MRI could be advocated only in inconclusive cases. Uneven implant shell was found to be the most important US sign of breast implant rupture. Based on the findings, we recommend performing US examination after breast augmentation surgery with silicone gel-filled implants annually.
Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
Authors: Stupar Petar , Podolski-Renić Ana , Villalba Maria Ines , Dragoj Miodrag , Jovanović Stojanov Sofija , Pešić Milica , Kasas Sandor
Published: May 2021

Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer worldwide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. Materials and Methods: Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. Results: The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. Conclusions: Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method’s applicability.
The Effects of Prednisone/Ketoprofen Administration in Association with Amoxicillin Clavulanate Following Periodontal Surgical Therapy in Patients with Severe Chronic Periodontitis
Authors: Sfetcu Lidia , Didilescu Andreea Cristiana , Vlădan Cristian , Dincă Octavian , Miricescu Daniela , Băncescu Gabriela , Bucur Alexandru , Tribus Laura Carina
Published: May 2021

Background and Objectives: The aim of this study was to evaluate and compare the effects of two different anti-inflammatory drugs (ketoprofen and prednisone) combined with an antibiotic (amoxicillin + clavulanic acid) and periodontal surgery on dental and periodontal parameters in patients with severe chronic periodontitis. In addition, salivary stress expressed by cortisol levels was assessed. Materials and Methods: An interventional study was performed on 22 periodontal subjects and 19 clinical healthy controls. The patients were divided in four groups, depending on treatment planning, as follows: eight patients received prednisone and antibiotherapy, associated with surgical periodontal therapy; seven patients received ketoprofen and antibiotherapy, associated with surgical periodontal therapy (group II); seven patients received only prednisone. Periodontal healthy patients underwent routine scaling and polishing. Bleeding on probing (BOP), dental mobility and salivary cortisol (ng/mL) were assessed before and after treatment. The means and standard deviations for the salivary cortisol levels (SCLs), dental and periodontal parameters were calculated for all groups using each patient as a unit of analysis. Results: Data analyses showed that the two different anti-inflammatory drugs associated with or without surgical therapy were efficient on inflammation periodontal parameters (BOP, dental mobility). Prednisone treatment alone was associated with a significant decrease of SCLs between pretreatment and post-treatment. Conclusions: In the present study, the effects of either of the anti-inflammatory drugs on inflammation evolution and salivary stress were comparable in patients undergoing antibiotherapy and surgical periodontal therapy.
The Phase Stability of Al3Er Studied by the First-Principles Calculations and Experimental Analysis
Authors: Gao Chunlai , Gao Kunyuan , Ding Yusheng , Li Haonan , Wu Xiaolan , Wen Shengping , Gao Mu , Huang Hui , Nie Zuoren , Zhou Dejing
Published: May 2021

The thermodynamics of five Al3Er compounds were investigated through first-principles density-functional theory (DFT) and experimental analysis. The Al3Er compounds with Al3Ho.hR20 (prototype Al3Ho, Pearson symbol hR20), Cu3Au.cP4, AlNd3.hP8, Ni3Ti.hP16 and Al3Gd.hR12 structures exhibited formation energies of −0.412(−0.417), −0.411(−0.416), −0.400(−0.413), −0.399(−0.345) and −0.342(−0.345) meV/atom when using DFT with “standard” potential (“frozen core” potential) of Er. The results indicated that the Al3Ho.hR20 structure was the thermodynamic stable phase and the other structures were metastable. The formation energy of Cu3Au.cP4 structure was only 1 meV/atom less than that of Al3Ho.hR20. Experimentally, the Al-30 wt.% Er alloys were cooled from 900 °C to 500 °C at the rate of 5 ± 2 °C/h and 60 ± 2 °C/h, respectively. The corresponding XRD analysis showed that the Al3Ho.hR20 was formed at the cooling rate of 5 ± 2 °C/h and the Cu3Au.cP4 was formed at the cooling rate of 60 ± 2 °C/h, which indicated that the Al3Ho.hR20 was in a thermodynamic stable phase and the Cu3Au.cP4 was in a metastable phase with high stability. The structural analysis indicated that the tiny energy difference between Al3Ho.hR20 and Cu3Au.cP4 might be attributed to a similar structure with varied stacking sequences.
The Impact of Microbiome and Microbiota-Derived Sodium Butyrate on Drosophila Transcriptome and Metabolome Revealed by Multi-Omics Analysis
Authors: Zhou Fan , Liu Biaodi , Liu Xin , Li Yan , Wang Luoluo , Huang Jia , Luo Guanzheng , Wang Xiaoyun
Published: May 2021

The host microbiome plays an important role in regulating physiology through microbiota-derived metabolites during host-microbiome interactions. However, molecular mechanism underly host-microbiome interactions remains to be explored. In this study, we used Drosophila as the model to investigate the influence of microbiome and microbiota-derived metabolite sodium butyrate on host transcriptome and metabolome. We established both a sterile Drosophila model and a conventional Drosophila model to demonstrate the role of sodium butyrate. Using multi-omics analysis, we found that microbiome and sodium butyrate could impact host gene expression patterns in both the sterile Drosophila model and the conventional Drosophila model. The analysis of gut microbial using 16S rRNA sequencing showed sodium butyrate treatment also influenced Drosophila bacterial structures. In addition, Drosophila metabolites identified by ultra-high performance liquid chromatography-MS/MS were shown to be affected by sodium butyrate treatment with lipids as the dominant changed components. Our integrative analysis of the transcriptome, the microbiome, and the metabolome data identified candidate transcripts that are coregulated by sodium butyrate. Taken together, our results reveal the impact of the microbiome and microbiota-derived sodium butyrate on host transcriptome and metabolome, and our work provides a better understanding of host-microbiome interactions at the molecular level with multi-omics data.
IDARE2—Simultaneous Visualisation of Multiomics Data in Cytoscape
Authors: Pfau Thomas , Galhardo Mafalda , Lin Jake , Sauter Thomas
Published: May 2021

Visual integration of experimental data in metabolic networks is an important step to understanding their meaning. As genome-scale metabolic networks reach several thousand reactions, the task becomes more difficult and less revealing. While databases like KEGG and BioCyc provide curated pathways that allow a navigation of the metabolic landscape of an organism, it is rather laborious to map data directly onto those pathways. There are programs available using these kind of databases as a source for visualization; however, these programs are then restricted to the pathways available in the database. Here, we present IDARE2 a cytoscape plugin that allows the visualization of multiomics data in cytoscape in a user-friendly way. It further provides tools to disentangle highly connected network structures based on common properties of nodes and retains structural links between the generated subnetworks, offering a straightforward way to traverse the splitted network. The tool is extensible, allowing the implementation of specialised representations and data format parsers. We present the automated reproduction of the original IDARE nodes using our tool and show examples of other data being mapped on a network of E. coli. The extensibility is demonstrated with two plugins that are available on github. IDARE2 provides an intuitive way to visualise data from multiple sources and allows one to disentangle the often complex network structure in large networks using predefined properties of the network nodes.
Simplified Method of Microcontact Force Measurement by Using Micropressure Sensor
Authors: Zhu Huamin , Zheng Fuzhong , Leng Huiwen , Zhang Cheng , Luo Kun , Cao Yibo , Yang Xing
Published: May 2021

Microcontact force measurement is widely applied in micro/nano manufacturing, medicine and microelectromechanical systems. Most microcontact force measurements are performed by using mass comparators, nano-indenter and precision electronic balance, and weighing sensors. However, these instruments have a complex structure and high cost. Nevertheless, the rapid development of microsensor technology provides a new, simple and low-cost approach for microcontact force measurement. In this study, we present a method of microcontact force measurement by using micropressure sensors and study the relationship amongst the microcontact force, output voltage and contact position of the sensor. We use a microcapacitance pressure sensor as an example, then we perform a simulation calculation and construct a microcontact force experiment system to verify the simulation results. The experimental and simulation results are consistent. In addition, an equation that describes the relationship amongst the microcontact force, output voltage and contact position of the sensor is obtained. Based on this simple and low-cost method, we build a micro-manipulation system, which indicates that the micropressure sensors can be used to measure microcontact force in various applications easily and cost-effectively. Furthermore, it is considerably relevant to research and application in this field.
A Novel Preparation of Ag Agglomerates Paste with Unique Sintering Behavior at Low Temperature
Authors: Li Junlong , Xu Yang , Meng Ying , Yin Zhen , Zhao Xuelong , Wang Yinghui , Suga Tadatomo
Published: May 2021

A novel bonding process using Ag agglomerates paste prepared by Ag2O reduction has been proposed, which solved the problem of Cu substrate oxidation in the conventional Ag2O sintering process for Cu–Cu bonding. By applying the Ag agglomerate paste to Ag–Ag bonding, a shear strength of 28.3 MPa at 150 °C was obtained. Further studies showed that the optimum sintering temperature was at 225 °C, and a shear strength of 46.4 MPa was obtained. In addition, a shear strength of 20 MPa was obtained at 225 °C for Cu–Cu bonding. Compared to common Ag pastes, the results in this paper revealed that the sintering behavior of Ag agglomerates was unique, and the sintering mechanisms for Ag–Ag and Cu–Cu bonding were also discussed.