bluuebunny/crossref_metadata_2025 huggingface dataset:
Sequential embryo transfer versus double cleavage-stage embryo or double blastocyst transfer in patients with recurrent implantation failure with frozen-thawed embryo transfer cycles: a cohort study
Authors: Gao Jiangman , Yuan Yifeng , Li Jia , Tian Tian , Lian Ying , Liu Ping , Li Rong , Qiao Jie , Long Xiaoyu , Wang Haiyan
Published: September 2023

Background

Recurrent implantation failure (RIF) is more common among patients receiving assisted reproductive treatment. Many efforts have been made to increase the incidence of clinical pregnancy among patients with RIF. The effect of the sequential transfer procedure, a two-step interval transfer of a cleavage-stage embryo followed by a blastocyst in one transfer cycle, on the clinical outcomes of RIF patients remains controversial.

Methods

In total, 1774 frozen-thawed embryo transfer (FET) cycles in RIF patients were included. Of these cycles, 302 were sequential embryo transfer (ET) cycles, 979 were double day 3 cleavage-stage ET cycles, and 493 were double blastocyst ET cycles. The primary outcomes were the rates of implantation, clinical pregnancy and multiple pregnancy, and the secondary outcomes were the rates of hCG positive, early miscarriage and ectopic pregnancy.

Results

The implantation, hCG positive, and clinical pregnancy rates in the sequential ET group (32.1%, 58.9%, 50.7%) were significantly higher than those in the day 3 cleavage-stage ET group (24.9%, 46.5%, 40.4%) and were similar to those in the blastocyst transfer group (30.1%, 56.4%, 47.1%). The early miscarriage rate in the blastocyst transfer group was significantly higher than that in the cleavage-stage ET group (17.2% vs. 8.1%, P <0.05), while the ectopic pregnancy rate in the blastocyst transfer group was significantly lower than that in the cleavage-stage ET group (0.4% vs. 3.0%, P <0.05). The multiple pregnancy rate in the sequential ET group was significantly lower than that in the cleavage-stage ET group (17.0% vs. 25.5%, P <0.05) and the blastocyst transfer group (17.0% vs. 27.6%, P <0.05). When cycles of blastocyst culture failure were excluded, the clinical pregnancy rate was significantly higher (55.7% vs. 47.1%, P <0.05), and the early miscarriage rate and multiple pregnancy rate were significantly lower (8.5% vs. 17.2%, 17.7% vs. 27.6%; P <0.05, respectively) in the sequential ET group than in the double blastocyst ET group.

Conclusions

Sequential embryo transfer in FET cycles could improve the clinical outcomes of patients with RIF.
Modulation of immune response by nanoparticle-based immunotherapy against food allergens
Authors: Krishna Sivadas Swathi , Farhana Syeda Ayesha , T.P. Ardra , Hussain Shalam M. , Viswanad Vidya , Nasr Muhammed Hassan , Sahu Ram Kumar , Khan Jiyauddin
Published: September 2023

The increasing prevalence of food allergies worldwide and the subsequent life-threatening anaphylactic reactions often have sparse treatment options, providing only symptomatic relief. Great strides have been made in research and in clinics in recent years to offer novel therapies for the treatment of allergic disorders. However, current allergen immunotherapy has its own shortcomings in terms of long-term efficacy and safety, due to the local side effects and the possibility of anaphylaxis. Allergen-specific immunotherapy is an established therapy in treating allergic asthma, allergic rhinitis, and allergic conjunctivitis. It acts through the downregulation of T cell, and IgE-mediated reactions, as well as desensitization, a process of food tolerance without any allergic events. This would result in a protective reaction that lasts for approximately 3 years, even after the withdrawal of therapy. Furthermore, allergen-specific immunotherapy also exploits several routes such as oral, sublingual, and epicutaneous immunotherapy. As the safety and efficacy of allergen immunotherapy are still under research, the exploration of newer routes such as intra-lymphatic immunotherapy would address unfulfilled needs. In addition, the existence of nanoparticles can be exploited immensely in allergen immunotherapy, which would lead to safer and efficacious therapy. This manuscript highlights a novel drug delivery method for allergen-specific immunotherapy that involves the administration of specific allergens to the patients in gradual increasing doses, to induce desensitization and tolerance, as well as emphasizing different routes of administration, mechanism, and the application of nanoparticles in allergen-specific immunotherapy.
A deeper dive into the blue economy: the role of the diving sector in conservation and sustainable development goals
Authors: Forrest Matthew J. , Favoretto Fabio , Nisa Zahidah A. , Aburto-Oropeza Octavio
Published: September 2023

Attaining an equitable Blue Economy requires reconsidering historical extractive usages of natural ocean capital in favor of more sustainable activities. Scuba diving is an expanding industry, and several examples illustrate how the diving sector has assisted with transitions to sustainable economic activities. In certain countries diving tourism generates revenues comparable with fishing industries, yet the sector remains underrepresented within marine conservation efforts. Therefore, we present five actions tailored to enhance the diving sector’s participation in the Blue Economy: i) Organize the fragmented sector via international associations and federations; ii) Recognize usage rights for natural capital equal to extractive activities; iii) Modernize the sector using technology to improve connectivity and data sharing; iv) Invest in the sector by engaging private and public funding and subsidizing critical infrastructure to enable equitable access; v) Foster a sense of community by training and supporting local leaders, thereby ensuring more equitable participation by including women, indigenous people, and the youth. Diving represents one of the only endeavors that enables citizens to actively support the Blue Economy and help to achieve the United Nations Sustainable Development Goal 14, “Life Below Water”; therefore, the diving sector is uniquely poised to help address conservation goals and sustainable development.
Temperature-dependent feeding behavior in the brown box crab, Lopholithodes foraminatus
Authors: Moretto Wave I. , Taylor Jennifer R. A.
Published: September 2023

Introduction

Growing fishing pressures and ocean warming are potential concerns for populations of brown box crabs, Lopholithodes foraminatus, at the southern end of their geographic distribution. In Southern California, brown box crabs are thought to occupy a broad depth gradient (intertidal zone - 547 m), which exposes them to temperatures ranging from 8°C - 24°C. This large temperature span presents challenges for these ectothermic animals because the rates of their physiological processes, and ultimately their dietary needs, behavior, and health, vary with temperature. Here we examined how temperature affects the feeding behavior of brown box crabs to better understand their ecology in warmer regions of their distribution.

Methods

Adult crabs were exposed to one of three temperature treatments (8°C, 15°C, and 20°C, N=10) for 2 months. Weekly throughout the exposure period, crabs were given two similar-sized prey items of different crushing strength (clam and mussel). Claw pinch force, prey preference, time to consume prey, and number of prey consumed were recorded prior to the start of the experiment and weekly for the duration of the study, with the exception of pinch force, which was recorded bi-weekly. We hypothesized that crabs will have a stronger pinch force at warmer temperatures, prefer prey items that require less breaking force at the coldest temperature, consume prey faster at warmer temperatures, and consume more prey at warmer temperatures.

Results

Our results confirm that pinch force is consistently greater at 15°C compared to 8°C, however, crabs at 8°C consumed more clams (higher strength) while those at 15°C consumed more mussels. Crabs at 8°C also consumed prey items faster than crabs at 15°C but ate a similar number of prey. Furthermore, there was 100% mortality at 20°C within 8 days of exposure, indicating their proximity to lethal limits within Southern California.

Discussion

These results show that brown box crab feeding behavior exhibits temperature thresholds, which may alter their nutritional state, community interactions, and distribution under both short-term and long-term changes in ocean temperature.
Effects of the particle morphology and volume fraction of coarse aggregate on the mechanical properties of concrete
Authors: Zhao Wei , Si Zheng , Du Xiaoqi , Huang Lingzhi
Published: September 2023

The morphology and volume fraction of coarse aggregate particles significantly influence the mechanical characteristics of concrete. This paper presents the development of a numerical model for pebble concrete by combining experiments with the discrete element method. The mesoscopic parameters between mortars and the contact surface between mortars and aggregates in the model were calibrated by comparing simulated stress–strain relationships with experimental data from pebble concrete. Subsequently, the model and mesoscopic parameters were validated by comparing them with experimental results. The obtained mesoscopic parameters were then utilized in the numerical modeling of uniaxial compression for single-shaped coarse aggregate concrete. Results showed that the peak stress of the elliptical aggregate concrete was 2.8 MPa higher than that of the oblate spherical aggregate concrete, leading to more cracks and severe damage in the former. Thus, elliptical aggregates proved more suitable as coarse aggregates for concrete than oblate spherical aggregates. Furthermore, the study investigated the influence of ellipsoidal aggregate volume fraction on concrete strength. The compressive strength reached the highest when the volume fraction of ellipsoidal aggregate was 57%. Therefore, the ideal coarse aggregate volume fraction was determined to be 57%. These results offer valuable insights into designing concrete mix proportions.
Predictors and outcomes of recurrent retroperitoneal liposarcoma with multiple tumors
Authors: Deng Huan , Xu Xingming , Gao Jingwang , Huang Jun , Liu Guibin , Song Liqiang , Wei Bo
Published: September 2023

Background

Retroperitoneal liposarcoma (RLS) is a rare but severe disease. Repeated postoperative recurrence with multiple tumors is a therapeutic dilemma. The clinical outcomes and survival predictors of recurrent RLS with multiple tumors remain to be explored.

Methods

Patients with recurrent RLS were retrospectively analyzed. Univariate and multivariate analysis was performed to find independent prognostic factors that were correlated with Overall survival (OS) or progression-free survival (PFS). Factors significant in univariate analysis were further included into multivariate Cox proportional hazards regression model. The nomogram model was built to predict the survival status of patients. Variables that were significant in multivariable analysis were added to the internally validated nomogram models. The analysis of OS and PFS was performed by Kaplan–Meier analysis and log-rank test.

Results

A total of 113 recurrent RLS patients with multiple tumors were enrolled in the study. The 1-, 3-, and 5-years OS (PFS) rates were 70.7% (76.1%), 35.9% (76.1%), and 30.9% (76.1%), respectively. Univariate and multivariate analyses showed that number of surgeries, resection methods, tumor size, status of pathological differentiation, pathological subtypes, and recurrence patterns were important prognostic factors for OS or PFS (each p < 0.05). Nomogram models were established to efficiently predict the prognostic status of patients. Patients with the local recurrence (LR) pattern had a poor prognosis and would derive no survival benefit from combined organ resection and R0/R1 resection (each p < 0.05).

Conclusion

RLS patients recurrence with multiple tumors had a poor prognosis. Those patients should be followed up more frequently after surgery. The strategies of aggressive resection may not improve the survival of patients with LR pattern in the retroperitoneum. Prognostic factors in the efficient nomogram models should be considered in the individualized clinical management of recurrent RLS with multiple tumors.
Postconditioning promotes recovery in the neurovascular unit after stroke
Authors: Esposito Elga , Licastro Ester , Cuomo Ornella , Lo Eng H. , Hayakawa Kazuhide , Pignataro Giuseppe
Published: September 2023

Background and purpose

Experimental studies suggest that ischemic postconditioning interferes with cell death mechanisms and reduces infarction during the acute phase after focal cerebral ischemia. Postconditioning may be a practically feasible way to promote stroke recovery, but many drawbacks prevent its clinical translation. First, all existing studies are mostly on acute 24 h outcomes. Second, the mechanisms of protection and augmented long-term benefits remain unclear. Our study aims to define some of the mechanisms that explain long-term benefits of improved recovery.

Methods

Male Sprague–Dawley rats were subjected to 100-min transient middle cerebral artery occlusion (MCAO) or postconditioning (100-min middle cerebral artery occlusion plus 10-min reperfusion plus 10-min reocclusion). After 3 days or 2 weeks, infarct volumes, western blot, and immunohistochemical markers of neurogenesis and angiogenesis were quantified. Fluorocitrate (FC) or saline were administrated ICV (intraventricular injection) every other day starting on day 5 after focal cerebral ischemia, animals were recovered for 2 weeks.

Results

After postconditioning BDNF protein expression levels increased compared to animals subjected to MCAO. Immunostaining showed that BDNF increased specifically in astrocytes. Moreover, when astrocytes were metabolically inhibited by fluorocitrate the postconditioning neuroprotective effect together with the postconditioning-dependent new angiogenesis and neurogenesis, were no longer observed.

Conclusion

These results suggest for the first time that therapeutic effects of postconditioning may involve the promotion of neurogenesis and angiogenic remodeling, via BDNF released by astrocytes, during the recovery phase after focal cerebral ischemia.
Targeting neuroendocrine abnormalities in Parkinson’s disease with exercise
Authors: Luthra Nijee S. , Christou Demetra D. , Clow Angela , Corcos Daniel M.
Published: September 2023

Parkinson’s Disease (PD) is a prevalent and complex age-related neurodegenerative condition for which there are no disease-modifying treatments currently available. The pathophysiological process underlying PD remains incompletely understood but increasing evidence points to multiple system dysfunction. Interestingly, the past decade has produced evidence that exercise not only reduces signs and symptoms of PD but is also potentially neuroprotective. Characterizing the mechanistic pathways that are triggered by exercise and lead to positive outcomes will improve understanding of how to counter disease progression and symptomatology. In this review, we highlight how exercise regulates the neuroendocrine system, whose primary role is to respond to stress, maintain homeostasis and improve resilience to aging. We focus on a group of hormones – cortisol, melatonin, insulin, klotho, and vitamin D – that have been shown to associate with various non-motor symptoms of PD, such as mood, cognition, and sleep/circadian rhythm disorder. These hormones may represent important biomarkers to track in clinical trials evaluating effects of exercise in PD with the aim of providing evidence that patients can exert some behavioral-induced control over their disease.
Prediction of prognosis, immune infiltration, and personalized treatment of hepatocellular carcinoma by analysis of cuproptosis-related long noncoding RNAs and verification in vitro
Authors: Li Shanbao , Zhu Zhonglin , Lu Jing , Cao Wanyue , Song Fangbin , Xiao Cao , Zhang Peng , He Zeping , Weng Junyong , Xu Junming
Published: September 2023

Background

The correlations between cuproptosis and long noncoding RNAs (lncRNAs) with the tumor microenvironment (TME), immunotherapy, and some other characteristics of hepatocellular carcinoma (HCC) remain unclear.

Methods

Sixteen cuproptosis regulators and 356 cuproptosis-related lncRNAs (CRLnc) were identified from 374 HCC profiles in The Cancer Genome Atlas (TCGA) database. Six differentially expressed CRLnc were selected, and a prognostic risk model based on the CRLnc signature (CRLncSig) was constructed. The prognostic power of the model was verified. Moreover, a cuproptosis-related gene cluster (CRGC) was generated based on six lncRNAs and differentially expressed genes. The relationship between immune cell infiltration in the TME, immunotherapy, CRLncSig, and CRGC was demonstrated through various algorithms, Tumor Immune Dysfunction and Exclusion (TIDE), tumor mutational burden (TMB), etc. Potential drugs and sensitivity to those agents were evaluated for the risk model. LncRNA AL158166.1 was selected and verified in HCC tissues and cell lines, the impact of its knockdown and overexpression in HCC cells was examined, and the copper (Cu) concentration and the cuproptosis-related gene expression were detected.

Results

A CRLncSig prognostic risk model with good predictive ability was constructed. The low-risk group had a longer overall survival (OS), lower tumor purity, more extensive immune cell infiltration, higher immune score, enrichment in immune-activated pathways, and more positive response to immunotherapy versus the high-risk group. CRGC-B exhibited the best OS and the lowest tumor stage; the immune cell infiltration analysis was similar to the low-risk group in CRLncSig. CRGC-B belonged to the “immune-high” group of the TME. The low-risk group had a higher TIDE score and susceptibility to antitumor drugs. The lncRNA AL158166.1 had the highest hazard ratio. The levels of AL158166.1 were higher in HCC tissues versus healthy tissues. Knockdown of AL158166.1 could lead to an increase in intracellular Cu concentration, induce DLAT low expression, and inhibit the proliferation and migration of HCC cells, whereas overexpression of AL158166.1 exerted the reverse effect.

Conclusion

Overall, a new CRLncSig prognostic risk model and a cuproptosis-related molecular signature were constructed and evaluated. The model and signature were associated with the prognosis, immune infiltration, and immunotherapy of HCC. Inhibiting the lncRNA AL158166.1 may induce cuproptosis and showed potential for the inhibition of tumors. Evaluation of the CRLnc, CRLncSig, and CRGC may enhance our understanding of the TME, determine the effectiveness of immunotherapy, and act as a marker for the prognosis of HCC.
Thoracoscopic esophagectomy for thoracic esophageal cancer with right aortic arch and Kommerell diverticulum: a case report and literature review
Authors: Yu Zhao-Jun , Guo Ling-Wen , Huang Yang-Yun , Zhao Lilan , He Zi-Jie , Pan Xiao-Jie , Chen Wen-Shu
Published: September 2023

Background

Esophageal carcinoma accompanied by a right aortic arch (RAA) is very rare. When combined with Kommerell diverticulum (KD), a right aortic arch forms a vascular ring encircling both the esophagus and trachea. Due to abnormal anatomy of the upper mediastinum, it is very difficult to dissociate the esophagus and its surrounding tissues, especially the left recurrent laryngeal nerve. Herein, we report a case of successful thoracoscopic esophagectomy in an esophageal cancer patient concurrent with a RAA and KD.

Case presentation

A 62-year-old male patient was diagnosed with esophageal squamous carcinoma in the middle esophagus at clinical stage I (cT1N0M0) according to UICC-TNM classification 8th edition. Further examinations revealed RAA and KD. Based on the three-dimensional CT (3D-CT) reconstruction, a Mckeown esophagectomy via a left thoracoscopic approach in semi-prone position was performed. During the operation, the left recurrent laryngeal nerve was accurately exposed and well protected. Postoperatively, severe complications, including anastomotic leakage and recurrent laryngeal nerve palsy, were not observed. The patient was discharged 12 days after the surgery.

Conclusion

Preoperative 3D-CT reconstruction is useful to clarify the vascular malformation in esophageal cancer patients with RAA, and helpful to formulate a reasonable surgical approach.
Unveiling novel insights in prostate cancer through single-cell RNA sequencing
Authors: Yu Wenyue , Wang Chun , Shang Zhiqun , Tian Jing
Published: September 2023

Single-cell RNA sequencing (scRNA-seq) is a cutting-edge technology that provides insights at the individual cell level. In contrast to traditional bulk RNA-seq, which captures gene expression at an average level and may overlook important details, scRNA-seq examines each individual cell as a fundamental unit and is particularly well-suited for identifying rare cell populations. Analogous to a microscope that distinguishes various cell types within a tissue sample, scRNA-seq unravels the heterogeneity and diversity within a single cell species, offering great potential as a leading sequencing method in the future. In the context of prostate cancer (PCa), a disease characterized by significant heterogeneity and multiple stages of progression, scRNA-seq emerges as a powerful tool for uncovering its intricate secrets.
Synergism of the receptor tyrosine kinase Axl with ErbB receptors mediates resistance to regorafenib in hepatocellular carcinoma
Authors: Breitenecker Kristina , Hedrich Viola , Pupp Franziska , Chen Doris , Řezníčková Eva , Ortmayr Gregor , Huber Heidemarie , Weber Gerhard , Balcar Lorenz , Pinter Matthias , Mikulits Wolfgang
Published: September 2023

Introduction

Hepatocellular carcinoma (HCC) patients at advanced stages receive immunotherapy or treatment with tyrosine kinase inhibitors (TKIs) such as Sorafenib (Sora) or Lenvatinib in frontline as well as Regorafenib (Rego) or Cabozantinib in second-line. A major hindrance of TKI therapies is the development of resistance, which renders drug treatment futile and results in HCC progression.

Methods

In this study, we addressed the impact of the receptor tyrosine kinase Axl binding to its ligand Gas6 in acquiring refractoriness to TKIs. The initial responses of Axl-positive and Axl-negative cell lines to different TKIs were assessed. Upon inducing resistance, RNA-Seq, gain- and loss-of-function studies were applied to understand and intervene with the molecular basis of refractoriness. Secretome analysis was performed to identify potential biomarkers of resistance.

Results

We show that HCC cells exhibiting a mesenchymal-like phenotype were less sensitive to drug treatment, linking TKI resistance to changes in epithelial plasticity. Gas6/Axl expression and activation were upregulated in Rego-resistant HCC cells together with the induction of ErbB receptors, whereas HCC cells lacking Axl failed to stimulate ErbBs. Treatment of Rego-insensitive HCC cells with the pan-ErbB family inhibitor Afatinib rather than with Erlotinib blocking ErbB1 reduced cell viability and clonogenicity. Genetic intervention with ErbB2-4 but not ErbB1 confirmed their crucial involvement in refractoriness to Rego. Furthermore, Rego-resistant HCC cells secreted basic fibroblast growth factor (bFGF) depending on Axl expression. HCC patients treated with Sora in first-line and with Rego in second-line displayed elevated serum levels of bFGF, emphasizing bFGF as a predictive biomarker of TKI treatment.

Discussion

Together, these data suggest that the inhibition of ErbBs is synthetic lethal with Rego in Axl-expressing HCC cells, showing a novel vulnerability of HCC.
A type II cannabis extract and a 1:1 blend of Δ(9)-tetrahydrocannabinol and cannabidiol display distinct antinociceptive profiles and engage different endocannabinoid targets when administered into the subarachnoid space
Authors: Benredjem Besma , Pineyro Graciela
Published: September 2023

Introduction: Cannabis extracts are being increasingly used to mitigate chronic pain. Current guidelines for their prescription rely on Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) content as well as the ratio of these major cannabinoids present in the blend. Here we assessed whether these descriptors were representative of product effectiveness to produce a desired outcome such as analgesia.

Methods: In this study, we used a rat model of diabetic neuropathy and assessed the reduction in mechanical allodynia following intrathecal injection of pure THC, pure CBD, a 1:1 mix of these compounds and a “balanced” chemotype II cannabis extract. Engagement of endocannabinoid targets by different treatments was investigated using CB1 (AM251) and CB2 (AM630) receptor antagonists as well as a TRPV1 channel blocker (capsazepine).

Results: Antinociceptive responses induced by an equivalent amount of THC administered in its pure form, as a THC:CBD mix or as a “balanced” extract were distinct. Furthermore, the 1:1 THC:CBD mix and the balanced extract had not only different response profiles but their relative engagement of CB1, CB2 receptors and TRPV1 channels was distinct.

Discussion: These findings indicate that antinociceptive responses and targets engaged by blended cannabinoids are composition-specific, and cannot be simply inferred from THC and CBD contents. This information may have implications in relation to the way medicinal cannabis products are prescribed.
M-shaped beam profile output from an off-axis relay-imaging eight-pass Nd:glass laser preamplifier
Authors: Xie Xudong , Yao Ke , Zhou Shouhuan
Published: September 2023

In ultra-high-power/energy solid-state Nd:glass laser facilities, the gain distribution of the main amplifier system (MAS) is nonuniform owing to strong amplified spontaneous emissions in the MAS. A shaped beam profile from a preamplifier (PreAMP) should be set opposite to the gain profile in the MAS to obtain a high-power beam with an approximately flat beam profile. This flat beam profile can result in the high efficiency of the frequency-conversion crystals. In this study, the laser beam was spatially formatted as an M-shaped beam with precompensation (PreCOM) for the nonuniform gain profile of the MSA. Modulation of the cross-sectional gain distribution of the PreAMP was demonstrated by selecting the absorption coefficient of the Nd:glass rod and pump configuration. Using the proposed off-axis relay-imaging eight-pass amplifier, an M-shaped beam with an output pulse energy of 1 J and a repetition rate of 1 Hz was experimentally achieved. With an Nd3+ concentration of 1.2 wt% and Nd: glass rod diameter of 18 mm, an M-shaped beam profile with a 4.3:1 ratio of intensity at the margin to that at the center was successfully obtained. Such controllable M-shaped beams can be optionally introduced to the MAS to compensate for non-uniform spatial gain profiles.
Exosomes as biomarkers and therapy in type 2 diabetes mellitus and associated complications
Authors: Satyadev Nihal , Rivera Milagros I. , Nikolov Nicole K. , Fakoya Adegbenro O. J.
Published: September 2023

Type 2 diabetes mellitus (T2DM) is one of the most prevalent metabolic disorders worldwide. However, T2DM still remains underdiagnosed and undertreated resulting in poor quality of life and increased morbidity and mortality. Given this ongoing burden, researchers have attempted to locate new therapeutic targets as well as methodologies to identify the disease and its associated complications at an earlier stage. Several studies over the last few decades have identified exosomes, small extracellular vesicles that are released by cells, as pivotal contributors to the pathogenesis of T2DM and its complications. These discoveries suggest the possibility of novel detection and treatment methods. This review provides a comprehensive presentation of exosomes that hold potential as novel biomarkers and therapeutic targets. Additional focus is given to characterizing the role of exosomes in T2DM complications, including diabetic angiopathy, diabetic cardiomyopathy, diabetic nephropathy, diabetic peripheral neuropathy, diabetic retinopathy, and diabetic wound healing. This study reveals that the utilization of exosomes as diagnostic markers and therapies is a realistic possibility for both T2DM and its complications. However, the majority of the current research is limited to animal models, warranting further investigation of exosomes in clinical trials. This review represents the most extensive and up-to-date exploration of exosomes in relation to T2DM and its complications.
Research on decoupled air speed and air volume adjustment methods for air-assisted spraying in orchards
Authors: Dou Hanjie , Zhai Changyuan , Zhang Yanlong , Chen Liping , Gu Chenchen , Yang Shuo
Published: September 2023

Different fruit tree canopies have different requirements for air speed and air volume. Due to the strong relationship between air speed and air volume, the decoupled control of air speed and air volume cannot be achieved using the existing sprayers. In this study, an innovative air-assisted sprayer that supports the independent adjustment of fan speed (0-2940 r/min) and air outlet area (1022.05-2248.51 cm2) is developed, and the maximum air speed and air volume of the sprayer outlet are 45.98 m/s and 37239.94 m3/h, respectively. An independent adjustment test of the fan speed and air outlet area was carried out. The results indicated that the fan speed and air outlet area have opposing adjustment effects on air speed and air volume; decoupled control of the outlet air speed and air volume can thus be achieved through combined control of the fan speed and air outlet area. A test was carried out on combined fan speed and air outlet area control. Two decoupled air speed and air volume adjustment models were established, one with a constant air speed and variable air volume and the other with a constant air volume and variable air speed. The test results show that the air volume adjustment model with constant air speed had a maximum mean error of 1.13%, and the air speed adjustment model with constant air volume had a maximum mean error of 1.67%. The results will provide theoretical and methodological support for the development of airflow adjustment systems for orchard air-assisted sprayer.
Emotion regulation among 4–6 year-old children and its association with their peer relationships in Jordan
Authors: Qashmer Amani F.
Published: September 2023

Specific characteristics and competencies are required for maintaining peer relationships, and this study hypothesizes that emotion regulation is one of the competencies. The current study aimed to investigate the association between emotion regulation and peer relationships in 4–6-year-old children, and examine the sex differences among them. This study examined sex differences in peer relationships and the emotion regulation ability of children aged 4–6 years. The study sample comprised 300 children aged 4–6 years [170 girls (56.7%) and 130 boys (43.3%)] studying in kindergarten and first grade in Jordan. As part of data collection, questionnaires were distributed to teachers. The Emotion Regulation Scale (ERC) was used to measure emotion regulation, and the Social Competence and Behavior Evaluation (SCBE) scale, a subscale of the SCBE, was utilized to assess peer relationships. The results revealed a positive relationship between emotion regulation and positive poles of peer relationships (integrated, calm, and pro-social) and a significant negative relationship between emotional lability/negativity and positive poles of peer relationships. These results can be used to design intervention programs to reduce aggressive behavior in children.
Students’ academic engagement during COVID-19 times: a mixed-methods study into relatedness and loneliness during the pandemic
Authors: Hendrick Laura , Opdenakker Marie-Christine , Van der Vaart Wander
Published: September 2023

The COVID-19 outbreak forced higher education students to study online-only. Previous research indicates that forced solitude or loneliness can cause a variety of problems for students, among which is reduced academic engagement. The Basic Psychological Needs Theory, a sub-theory of Self-Determination Theory, relates academic engagement to three basic psychological needs (autonomy, competence and relatedness), whereas varying theories on loneliness highlight the complexities of engaging in a learning environment whilst feeling lonely. As university staff members have been struggling to keep students on task since the COVID-19 outbreak, the need arose for more knowledge about to what extent students have felt lonely, frustrated or satisfied in their need for relatedness and to what extent this affected their academic engagement. A convergent Mixed Methods research study was conducted among university students (N = 228) and an online questionnaire was administered to collect both quantitative and qualitative data. A series of multiple hierarchical regression analyses were performed, considering demographic characteristics, to analyze the quantitative data. Qualitative data was coded using a hybrid approach of deductive and inductive coding. Themes were generated that depicted in-depth issues of relatedness, loneliness, and academic engagement. Quantitative analysis demonstrated the importance for academic engagement of both (a) ‘basic need satisfaction and frustration’ of relatedness in life and in ‘social study context’, and (b) feeling (emotionally) lonely. The negative impact of frustration of relatedness seemed to be dominant but also overlapped with the effects of loneliness. The qualitative outcomes support and complement these quantitative results. The results showed that students’ academic engagement suffered from the loss of a shared physical space and from uncertainty about university policies. For a minority of students, however, the relief from social obligations that came along with social distancing was a blessing in disguise.
Using the theory of planned behavior model to predict factors influencing breastfeeding behavior among preterm mothers at week 6 postpartum: the mediating effect of breastfeeding intention
Authors: Huang Rong , Han Hui , Ding Lijing , Zhou Yi , Hou Yanwen , Yao Xiao , Cai Chenting , Li Xiaohan , Song Jianqi , Zhang Shuying , Jiang Hui
Published: September 2023

Background

Exclusive breastfeeding (EBF) in the first 6 weeks postpartum is key to continued breastfeeding. This study aimed to explore the role of EBF-related predictors (particularly breastfeeding intention) in breastfeeding behavior among preterm mothers at week 6 postpartum based on the theory of planned behavior (TPB).

Methods

A total of 352 mothers of preterm infants were recruited, 340 of whom participated in this study. Prior to discharge, participants completed the Chinese versions of the modified Breastfeeding Attrition Predictive Tool, the Breastfeeding Knowledge Questionnaire (BKQ), the Infant Feeding Intention, and the Edinburgh Postnatal Depression Scale. Responses to the items of the Breastfeeding Behavioral Questionnaire (BBQ) were also collected by telephone at week 6 postpartum. The final analyses included 321 participants who completed the full two-wave data collection.

Results

The fitness indices of the modified TPB model were acceptable. Breastfeeding knowledge and EBF before discharge positively impacted breastfeeding intention, whereas depression had a negative impact. Before discharge, breastfeeding intention fully mediated the impacts of breastfeeding attitude, social and professional support, knowledge, depression, and EBF on breastfeeding behavior and partially mediated the influence of perceived breastfeeding control on breastfeeding behavior.

Conclusion

These findings indicate that TPB accurately predicts breastfeeding behavior among preterm mothers at week 6 postpartum, and breastfeeding intention is key to the above-mentioned EBF-related factors and breastfeeding behavior. The findings underline the need for further longitudinal studies and corresponding interventions for preterm mothers with a high risk of EBF attrition.
Appearance and performance factors associated with muscle building supplement use and favourable attitudes towards anabolic steroids in adolescent boys
Authors: Piplios Olivia , Yager Zali , McLean Siân A. , Griffiths Scott , Doley Jo R.
Published: September 2023

Introduction

The demand for appearance and performance enhancing substances, including muscle building supplements and anabolic androgenic steroids, is increasing in Australia. However, little is known about the associations between appearance and performance-based factors and appearance and performance enhancing substances (APES), particularly among adolescent boys. This study sought to examine (a) the prevalence of muscle building supplement use in a sample of adolescent boys and (b) how both performance and appearance factors relate to muscle building supplement use and favourable attitudes towards anabolic androgenic steroids in this sample.

Method

N = 488 adolescent boys aged 13–16 (Mage = 14.59) from nine Australian schools completed measures of supplement use, favourable attitudes towards using steroids, muscle dissatisfaction, body fat dissatisfaction, mesomorphic ideal internalisation, weight training, and sports participation. Hierarchical logistic regressions were used to examine cross-sectional correlates of muscle building supplement use and favourable attitudes towards using anabolic androgenic steroids.

Results

In the past three months, 12.7% of the sample had used muscle building supplements. Both appearance and performance-related factors – mesomorphic ideal internalisation and weight training – were related to muscle building supplement use. Only one appearance-related factor – body dissatisfaction – was related to favourable attitudes towards anabolic androgenic steroids.

Discussion

The findings from this study are important as they may help to guide intervention strategies regarding appearance and performance enhancing substance use by Australian adolescent boys, with the ultimate goal of ensuring this population’s safety.
Association of adverse childhood experiences and cortical neurite density alterations with posttraumatic stress disorder symptoms in autism spectrum disorder
Authors: Kitamura Soichiro , Matsuoka Kiwamu , Takahashi Masato , Yoshikawa Hiroaki , Minami Akihiro , Ohnishi Hiroki , Ishida Rio , Miyasaka Toshiteru , Tai Yumi , Ochi Tomoko , Tanaka Toshihiro , Makinodan Manabu
Published: September 2023

Background

Posttraumatic stress disorder (PTSD) can be a source of significant social and daily distress in autism spectrum disorder (ASD). Compared to typically developed (TD) individuals, people with ASD are at an increased risk of adverse childhood experiences (ACEs), which can result in abnormal neuronal development. However, whether or how ACEs influence abnormal neural development and PTSD symptoms in ASD has not been fully elucidated.

Methods

Thirty-nine TD individuals and 41 individuals with ASD underwent T1-weighted magnetic resonance imaging and neurite orientation dispersion and density imaging (NODDI), with axonal and dendritic densities assessed in terms of the orientation dispersion index and neurite density index (NDI), respectively. Voxel-based analyses were performed to explore the brain regions associated with PTSD symptoms, and the relationships between the severity of ACEs and PTSD symptoms and NODDI parameters in the extracted brain regions were examined.

Results

There was a significant positive association between PTSD symptom severity and NDI in the bilateral supplementary motor area; right superior frontal, left supramarginal, and right superior temporal gyrus; and right precuneus in the ASD group, but not in the TD group. ACE severity was significantly associated with NDI in the right superior frontal and left supramarginal gyrus and right precuneus in the ASD group. Moreover, NDI in the right precuneus mainly predicted the severity of PTSD symptoms in the ASD group, but not the TD group.

Conclusion

These results suggest that ACE-associated higher neurite density is of clinical importance in the pathophysiology of PTSD symptoms in ASD.
Thinking beyond the virus: perspective of patients on the quality of hospital care before and during the COVID-19 pandemic
Authors: Wong Eliza Lai-yi , Wang Kailu , Cheung Annie Wai-ling , Graham Chris , Yeoh Eng-kiong
Published: September 2023

Objectives

The COVID-19 pandemic has a huge impact on the healthcare system and affects the normal delivery of routine healthcare services to hospitalized patients. This study aimed to examine the differences in patient experience of hospital service before and during COVID-19 among the discharged adult population.

Methods

A territory-wide patient experience survey was conducted before and during COVID-19 (between October 2019 and April 2020) among patients discharged from the main acute and rehabilitation public hospitals in Hong Kong. A hierarchical ordinal logistic model was employed to examine the difference in multiple dimensions of patient experience, with adjustments of covariates.

Results

In total, 9,800 participants were recruited. During the pandemic, there was a marginally significant increase in overall care rating (AOR: 1.12, 95% CI: 0.99–1.27), and an improvement in the timeliness of admission. However, significant reductions in patients’ confidence in nurses were observed. Communication of information regarding medication side effects reduced significantly (AOR: 0.72, 95% CI: 0.64–0.82).

Conclusion

The patients hospitalized during the pandemic reported worse responsiveness in communication in their patient journey than those admitted before the pandemic. These findings will help develop appropriate strategies to address patients’ concerns in the new normal.
Molecular fingerprinting by multi-locus sequence typing identifies microevolution and nosocomial transmission of Candida glabrata in Kuwait
Authors: Asadzadeh Mohammad , Ahmad Suhail , Al-Sweih Noura , Khan Ziauddin
Published: September 2023

Backgrounds

Candida glabrata is a frequently isolated non-albicans Candida species and invasive C. glabrata infections in older patients are associated with high mortality rates. Opportunistic Candida infections in critically ill patients may be either endogenous or nosocomial in origin and this distinction is critical for effective intervention strategies. This study performed multi-locus sequence typing (MLST) to study genotypic relatedness among clinical C. glabrata isolates in Kuwait.

Methods

Candida glabrata isolates (n = 91) cultured from 91 patients were analyzed by MLST. Repeat isolates (n = 16) from 9 patients were also used. Antifungal susceptibility testing for fluconazole, voriconazole, caspofungin and amphotericin B (AMB) was determined by Etest. Genetic relatedness was determined by constructing phylogenetic tree and minimum spanning tree by using BioNumerics software.

Results

Resistance to fluconazole, voriconazole and AMB was detected in 7, 2 and 10 C. glabrata isolates, respectively. MLST identified 28 sequence types (STs), including 12 new STs. ST46 (n = 33), ST3 (n = 8), ST7 (n = 6) and ST55 (n = 6) were prevalent in ≥4 hospitals. Repeat isolates obtained from same or different site yielded identical ST. No association of ST46 with source of isolation or resistance to antifungals was apparent. Microevolution and cross-transmission of infection was indicated in two hospitals that yielded majority (57 of 91, 67%) of C. glabrata.

Conclusion

Our data suggest that C. glabrata undergoes microevolution in hospital environment and can be nosocomially transmitted to other susceptible patients. Thus, proper infection control practices during routine procedures on C. glabrata-infected patients may prevent transmission of this pathogen to other hospitalized patients.
Magnitude and direction of elbow torque asymmetries in manual wheelchair users
Authors: Biduski Grazieli Maria , Knihs Débora Aparecida , Nery de Oliveira Silas , Hoinaski Laís Peixoto , Rossato Mateus , Freitas Cíntia De La Rocha
Published: September 2023

The aims of the present study are to investigate the magnitude and direction of the elbow torque asymmetries in manual wheelchair users and to verify the agreement levels of the asymmetry's direction between different velocities and contraction modes in the isokinetic test. The sample was composed of 14 manual wheelchair users (four women, 10 men). The peak torque of the elbow flexors and extensors were measured on the dominant and non-dominant limbs, using a set of concentric/eccentric contractions at speeds of 60° s−1 and 180° s−1. Asymmetries were calculated by a specific equation, and the levels of agreement of the asymmetry's direction were calculated using Kappa coefficient. The main results showed a large variability in the magnitude of the asymmetries, ranging from −73.1% (ND) to 59.9% (D) between participants. The agreement levels of the elbow flexors and extensors between the different contraction modes were great (k = 0.71–0.85) for most of the velocities [except for flexors of 60° s−1 (k = 0.29)], but the agreement levels were only slight to fair (k = 0.16–0.31) for most of the contraction modes when comparing between velocities [except for flexors eccentric (k = 0.71)]. In conclusion, the elbow torque asymmetries are highly variable between subjects in terms of magnitude. In addition, in general, the limb favored by the asymmetry is the same when comparing between velocities, but not when comparing between contraction modes.
Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation
Authors: Serradilla Javier , Andrés Moreno Ane Miren , Talayero Paloma , Burgos Paula , Machuca Mariana , Camps Ortega Onys , Vallejo María Teresa , Rubio Bolívar Francisco Javier , Bueno Alba , Sánchez Alba , Zambrano Cristina , De la Torre Ramos Carlos Andrés , Rodríguez Olaia , Largo Carlota , Serrano Pilar , Prieto Bozano Gerardo , Ramos Esther , López Santamaría Manuel , Stringa Pablo , Hernández Francisco
Published: September 2023

Considering recent clinical and experimental evidence, expectations for using DCD-derived intestines have increased considerably. However, more knowledge about DCD procedure and long-term results after intestinal transplantation (ITx) is needed. We aimed to describe in detail a DCD procedure for ITx using normothermic regional perfusion (NRP) in a preclinical model. Small bowel was obtained from pigs donors after 1 h of NRP and transplanted to the recipients. Graft Intestinal samples were obtained during the procedure and after transplantation. Ischemia-reperfusion injury (Park-Chiu score), graft rejection and transplanted intestines absorptive function were evaluated. Seven of 8 DCD procedures with NRP and ITx were successful (87.5%), with a good graft reperfusion and an excellent recovery of the recipient. The architecture of grafts was well conserved during NRP. After an initial damage of Park-chiu score of 4, all grafts recovered from ischemia-reperfusion, with no or very subtle alterations 2 days after ITx. Most recipients (71.5%) did not show signs of rejection. Only two cases demonstrated histologic signs of mild rejection 7 days after ITx. Interestingly intestinal grafts showed good absorptive capacity. The study’s results support the viability of intestinal grafts from DCD using NRP, contributing more evidence for the use of DCD for ITx.
GPU Acceleration of CFD Simulations in OpenFOAM
Authors: Piscaglia Federico , Ghioldi Federico
Published: September 2023

We introduce algorithmic advancements designed to expedite simulations in OpenFOAM using GPUs. These developments include the following. (a) The amgx4Foam library, which connects the open-source AmgX library from NVIDIA to OpenFOAM. Matrix generation, involving tasks such numerical integration and assembly, is performed on CPUs. Subsequently, the assembled matrix is processed on the CPU. This approach accelerates the computationally intensive linear solver phase of simulations on GPUs. (b) Enhancements to code performance in reactive flow simulations, by relocating the solution of finite-rate chemistry to GPUs, which serve as co-processors. We present code verification and validation along with performance metrics targeting two distinct application sets, namely, aerodynamics calculations and supersonic combustion with finite-rate chemistry.