bluuebunny/crossref_metadata_2025 huggingface dataset:
Mitochondrial Dysfunction in Systemic Lupus Erythematosus: Insights and Therapeutic Potential
Authors: Poznyak Anastasia V. , Orekhov Nikolay A. , Churov Alexey V. , Starodubtseva Irina A. , Beloyartsev Dmitry F. , Kovyanova Tatiana I. , Sukhorukov Vasily N. , Orekhov Alexander N.
Published: September 2024

Systemic lupus erythematosus (SLE) is a complex autoimmune disorder characterized by the presence of various serum autoantibodies and multi-system effects, predominantly affecting young female patients. The pathogenesis of SLE involves a combination of genetic factors, environmental triggers, and pathogen invasions that disrupt immune cell activation, leading to the release of autoantibodies and chronic inflammation. Mitochondria, as the primary cellular powerhouses, play a crucial role in SLE development through their control of energy generation, reactive oxygen species (ROS) production, and cellular apoptotic pathways. Dysregulation of mitochondrial structure and function can contribute to the immune dysregulation, oxidative stress, and inflammation seen in SLE. Recent research has highlighted the impact of mitochondrial dysfunction on various immune cells involved in SLE pathogenesis, such as T-lymphocytes, B-lymphocytes, neutrophils, and plasmacytoid dendritic cells. Mitochondrial dysfunction in these immune cells leads to increased ROS production, disrupted mitophagy, and alterations in energy metabolism, contributing to immune dysregulation and inflammation. Moreover, genetic variations in mitochondrial DNA (mtDNA) and abnormalities in mitochondrial dynamics have been linked to the pathogenesis of SLE, exacerbating oxidative stress and immune abnormalities. Targeting mitochondrial function has emerged as a promising therapeutic approach for SLE. Drugs such as sirolimus, N-acetylcysteine, coenzyme Q10, and metformin have shown potential in restoring mitochondrial homeostasis, reducing oxidative stress, and modulating immune responses in SLE. These agents have demonstrated efficacy in preclinical models and clinical studies by improving disease activity, reducing autoantibody titers, and ameliorating organ damage in SLE patients. In conclusion, this review underscores the critical role of mitochondria in the pathogenesis of SLE and the potential of targeting mitochondrial dysfunction as a novel therapeutic strategy for improving outcomes in SLE patients. Further investigation into the mechanisms underlying mitochondrial involvement in SLE and the development of targeted mitochondrial therapies hold promise for advancing SLE treatment and enhancing patient care.
The Oral Microbiome of Peri-Implant Health and Disease: A Narrative Review
Authors: de Campos Kajimoto Natalia , de Paiva Buischi Yvonne , Mohamadzadeh Mansour , Loomer Peter
Published: September 2024

Peri-implantitis disease has increased significantly over the last years, resulting in increased failure of implants. Many factors may play a role in implant complications and failure, including ones related to the oral microbiota. This literature review aims to summarize the current knowledge of microbiome of implants in health and disease, focusing not only on the presence/absence of specific microbiota or on their relative abundance, but also on their phenotypic expression and their complex relationships with the host. The authors examined the MEDLINE database and identified key topics about peri-implant oral microbiome in health and disease. The peri-implant microbiome differs from that of the tooth, both in health and disease, as they are structurally and chemically different. The adhesion and formation of the peri-implant biofilm can be affected by the surface energy, topography, wettability, and electrochemical charges of the implant surface. In addition, the morphogenesis of the tissues surrounding the dental implant also differs from the tooth, making the dental implant more susceptible to bacterial infection. This interplay between the microbiome and the host immune system in peri-implant infections still needs to be elucidated.
One-Photon-Interference Quantum Secure Direct Communication
Authors: Li Xiang-Jie , Wang Min , Pan Xing-Bo , Zhang Yun-Rong , Long Gui-Lu
Published: September 2024

Quantum secure direct communication (QSDC) is a quantum communication paradigm that transmits confidential messages directly using quantum states. Measurement-device-independent (MDI) QSDC protocols can eliminate the security loopholes associated with measurement devices. To enhance the practicality and performance of MDI-QSDC protocols, we propose a one-photon-interference MDI QSDC (OPI-QSDC) protocol which transcends the need for quantum memory, ideal single-photon sources, or entangled light sources. The security of our OPI-QSDC protocol has also been analyzed using quantum wiretap channel theory. Furthermore, our protocol could double the distance of usual prepare-and-measure protocols, since quantum states sending from adjacent nodes are connected with single-photon interference, which demonstrates its potential to extend the communication distance for point-to-point QSDC.
Systematic Literature Review on Pipeline Transport Losses of Hydrogen, Methane, and Their Mixture, Hythane
Authors: Hora Cristina , Dan Florin Ciprian , Secui Dinu-Calin , Hora Horea Nicolae
Published: September 2024

The transition to cleaner energy sources necessitates an in-depth understanding of the transport characteristics, losses, and opportunities associated with various gaseous fuels, including hydrogen, methane, and their mixtures, such as hythane. Hydrogen (H2), the most abundant element in the universe, is increasingly recognized as a viable alternative to fossil fuels, primarily due to its potential to reduce carbon footprints as a cleaner energy source. Gradually gaining prominence in the energy market, it is displacing other fuels such as methane. In some transport systems, hydrogen is mixed with methane (CH4) in order to reduce the carbon footprint while using the same existing production equipment. As more and more large methane consumers are implementing this mixture, we would like to see how the research has followed the market trend. An up-to-date research, development, and implementation status review is critical. This study aims to identify the main indicators of H2 and CH4 transport losses in pipes, providing a review of the state of the art in the specific literature. To deliver this, a systematic literature review (SLR) was carried out using preferred reporting items for systematic reviews and meta-analyses (PRISMA) methodology, pinpointing the research trends and results in peer review-published articles over a period of twelve years (2012–2024). Findings: this review identifies and points out, in numbers, the boundaries of the 2012–2024 timeline research.
Towards Renewable Energy Transition: Insights from Bibliometric Analysis on Scholar Discourse to Policy Actions
Authors: Diaconescu Mirela , Marinas Laura Elena , Marinoiu Ana Maria , Popescu Maria-Floriana , Diaconescu Mihai
Published: September 2024

Mounting climate concerns are making energy transition inevitable. Providing a reliable, cost-effective energy supply that meets the needs of all, as set up by Sustainable Development Goal 7, and promotes climate neutrality, as set up by the European Green Deal, is a complex task that requires complex and combined interventions in various sectors and policy areas. This paper aims to conduct a systematic analysis of the scholarly work focusing on energy transition towards renewables and to contribute to the existing knowledge by offering a holistic perspective on the dynamic landscape of energy transformation and the transition to renewables. To this end, advanced bibliometric techniques, combined with a systematic in-depth review of the existing literature and desk research, are used to uncover the intellectual landscape and identify influential works and emerging themes within this critical intersection of the economic, governance, political, social, and climate dimensions of energy transition. This analysis not only highlights prevailing trends and influential works but also sets the stage for future research and discussions critical to shaping the transition to renewable energy and policy actions in a rapidly evolving world. The results are useful guidance in the formulation of policy actions.
Dynamic Modeling and Control Strategy Optimization of a Volkswagen Crafter Hybrid Electrified Powertrain
Authors: Babangida Aminu , Szemes Péter Tamás
Published: September 2024

This article studies the transformation and assembly process of the Volkswagen (VW) Crafter from conventional to hybrid vehicle of the department of vehicles engineering, University of Debrecen, and uses a computer-aided simulation (CAS) to design the vehicle based on the real measurement data (hardware-in-the-loop, HIL method) obtained from an online CAN bus data measurement platform using MATLAB/Simulink/Simscape and LabVIEW software. The conventional vehicle powered by a 6-speed manual transmission and a 4-stroke, 2.0 Turbocharged Direct Injection Common Rail (TDI CR) Diesel engine and the transformed hybrid electrified powertrain are designed to compare performance. A novel methodology is introduced using Netcan plus 110 devices for the CAN bus analysis of the vehicle’s hybrid version. The acquired raw CAN data is analyzed using LabVIEW and decoded with the help of the database (DBC) file into physical values. A classical proportional integral derivative (PID) controller is utilized in the hybrid powertrain system to manage the vehicle consumption and CO2 emissions. However, the intricate nonlinearities and other external environments could make its performance unsatisfactory. This study develops the energy management strategies (EMSs) on the basis of enhanced proportional integral derivative-based genetic algorithm (GA-PID), and compares with proportional integral-based particle swarm optimization (PSO-PI) and fractional order proportional integral derivative (FOPID) controllers, regulating the vehicle speed, allocating optimal torque and speed to the motor and engine and reducing the fuel and energy consumption and the CO2 emissions. The integral time absolute error (ITAE) is proposed as a fitness function for the optimization. The GA-PID demonstrates superior performance, achieving energy efficiency of 90%, extending the battery pack range from 128.75 km to 185.3281 km and reducing the emissions to 74.79 gCO2/km. It outperforms the PSO-PI and FOPID strategies by consuming less battery and motor energy and achieving higher system efficiency.
Optimal Sizing and Economic Analysis of Community Battery Systems Considering Sensitivity and Uncertainty Factors
Authors: Ragab Ziad , Pashajavid Ehsan , Rajakaruna Sumedha
Published: September 2024

Efficient sizing and economic analysis of community battery systems is crucial for enhancing energy efficiency and sustainability in rooftop PV panel-rich communities. This paper proposes a comprehensive model that integrates key technical and economic factors to optimize the size and operation of the prosumer-owned battery, maximizing the financial returns over the life span of the battery. Sensitivity and uncertainty analyses were also conducted on a number of factors that are constantly changing over the years such as per-unit cost of the battery and interest rate. Monte Carlo simulations were utilized to replicate the unpredictable PV generations and the volatility of house load demands. The developed model is evaluated under three scenarios: a shared community battery for all houses, individual batteries for each house, and a combined system with an additional large load. Particle Swarm Optimization (PSO) is utilized to maximize the formulated objective function subject to the considered constraints. The findings indicate that integrating community batteries offered a substantial economic advantage compared to individual home batteries. The additional revenue stream of incorporating larger consumers looking to reduce their carbon footprint (e.g., commercial) returned a further augmented net present value (NPV). The influence of different tariff structures was also assessed and it was found that critical peak pricing (CPP) was the most prolific. The outcomes offer valuable insights for policymakers and stakeholders in the energy sector to facilitate a more sustainable future.
A 24-Step Short-Term Power Load Forecasting Model Utilizing KOA-BiTCN-BiGRU-Attentions
Authors: Xu Mingshen , Liu Wanli , Wang Shijie , Tian Jingjia , Wu Peng , Xie Congjiu
Published: September 2024

With the global objectives of achieving a “carbon peak” and “carbon neutrality” along with the implementation of carbon reduction policies, China’s industrial structure has undergone significant adjustments, resulting in constraints on high-energy consumption and high-emission industries while promoting the rapid growth of green industries. Consequently, these changes have led to an increasingly complex power system structure and presented new challenges for electricity demand forecasting. To address this issue, this study proposes a 24-step multivariate time series short-term load forecasting algorithm model based on KNN data imputation and BiTCN bidirectional temporal convolutional networks combined with BiGRU bidirectional gated recurrent units and attention mechanism. The Kepler adaptive optimization algorithm (KOA) is employed for hyperparameter optimization to effectively enhance prediction accuracy. Furthermore, using real load data from a wind farm in Xinjiang as an example, this paper predicts the electricity load from 1 January to 30 December in 2019. Experimental results demonstrate that our comprehensive short-term load forecasting model exhibits lower prediction errors and superior performance compared to traditional methods, thus holding great value for practical applications.
Review and Assessment of Decarbonized Future Electricity Markets
Authors: Darudi Ali , Weigt Hannes
Published: September 2024

The electricity sector plays a key role in achieving zero emissions targets. The required transition will lead to substantial changes in the supply, demand, and distribution of electricity, as well as in stakeholder roles. Future market designs may change substantially to accommodate these changes, address challenges, and take advantage of new opportunities. This paper reviews the characteristics of future carbon-neutral electricity systems and electricity market design options. To provide a guiding framework for the literature review, we transfer the complexity of electricity systems into a three-layer structure: Firstly, we analyze papers that rely on techno-economic modeling of the physical electricity system. As a case study, we analyze various studies focusing on a decarbonized European electricity system in 2050. Secondly, we review papers that investigate the economic behavior and effects of self-interest-seeking stakeholders such as producers, network operators, and consumers. Finally, we review papers focusing on policy and market design questions that guide policymakers in achieving a target physical asset combination while considering the behavior of stakeholders. We highlight common trends and disagreements in the literature, review the main drivers of future markets, and finally provide a mapping between those drivers, challenges, and opportunities. The review concludes that the most promising next step toward a fully comprehensive assessment approach is to combine existing approaches across topical and disciplinary boundaries.
Heat Transfer Enhancement in a 3D-Printed Compact Heat Exchanger
Authors: Kruzel Marcin , Bohdal Tadeusz , Dutkowski Krzysztof
Published: September 2024

The study describes experimental data on thermal tests during the condensation of HFE7100 refrigerant in a compact heat exchanger. The heat exchanger was manufactured using the additive 3D printing in metal. The material is AISI 316L steel. MPCM slurry was used as the heat exchanger coolant, and water was used as the reference medium. The refrigerant was condensed on a bundle of circular tubes made of steel with an internal/external diameter of di/de = 2/3 mm, while a mixture of water and phase change materials as the coolant flowed through the channels. Few studies consider the heat exchange in condensation using phase change materials; furthermore, there is also a lack of description of heat exchange in small-sized exchangers printed from metal. Most papers deal with computer research, including flow simulations of heat exchange. The study describes the process of heat exchange enhancement using the phase transition of coolant. Experimental data for the mPCM slurry coolant flow was compared to the data of pure water flow as a reference liquid. The tests were carried out under the following thermal and flow conditions: G = 10–450 [kg m−² s−1], q = 2000–25,000 [W m−²], and ts = 30–40 [°C]. The conducted research provided many quantities describing the heat exchange in compact heat exchangers, including heat exchanger heat power, heat exchange coefficient, and heat exchange coefficients for working media. Based on these factors, the thermal performance of the heat exchanger was described. External characteristics include the value of the thermal power and the heat exchange coefficient as a function of the mass flow density of the working medium and the average logarithmic temperature difference. The performance of the heat exchanger was presented as the dependencies of the heat exchange coefficients on the mass flux density and the heat flux density on the heat exchange surface. The thickness of the refrigerant’s condensate film was also determined. Furthermore, a model was proposed to determine the heat exchange coefficient value for the condensing HFE7100 refrigerant on the outer surface of a bundle of smooth tubes inside a compact heat exchanger. According to experimental data, the calculation results were in good agreement with each other, with a range of 25%. These data can be used to design mini condensers that are widely used in practice.
Experimental Investigation of Large-Scale Vertically Coated Tubes for Enhanced Air–Steam Condensation Heat Transfer
Authors: Chen Zengqiao , Zhang Keyuan , Wang Naihua
Published: September 2024

Non-condensable gas plays a significant role in steam condensation, primarily by reducing heat transfer efficiency. Enhanced condensation heat transfer in the presence of non-condensable gas is crucial for improving thermal efficiency, reducing energy consumption, and lowering costs. However, experimental studies on applying coatings to enhance condensation heat transfer in large-scale vertical outer tubes with non-condensable gas are scarce. This study investigates the condensation heat transfer performance of vertical stainless steel- and brass-coated tubes compared to their bare counterparts at different air concentrations (0.4, 0.3, 0.15, and 0.08). All tubes have an outer diameter of 19 mm and an effective length of 1080 mm. Visualizations reveal that condensate flow rates as high as 0.5 m/s on bare tubes cause significant disturbances to the diffusion layer. At various air concentrations, the maximum condensation heat transfer coefficient of the coated stainless steel tube exhibited increases of 22.2%, 11.9%, 4.2%, and 19.6% compared with the uncoated stainless steel tube. Similarly, the maximum condensation heat transfer coefficient for the coated brass tube showed significant increases of 58.9%, 53.5%, 68.0%, and 70.7% compared with the uncoated brass tube. Notably, the enhancement effect on heat transfer performance is more pronounced when the same type of modified surface is applied to the brass tube compared with the stainless steel tube.
Impact of Overpressure on the Preservation of Liquid Petroleum: Evidence from Fluid Inclusions in the Deep Reservoirs of the Tazhong Area, Tarim Basin, Western China
Authors: Su Peng , Zhang Jianyong , Zhou Zhenzhu , Chen Xiaolan , Zhang Chunrong
Published: September 2024

The complexity of petroleum phases in deep formations plays an important role in the evaluation of hydrocarbon resources. Pressure is considered to have a positive impact on the preservation of liquid oils, yet direct evidence for this phenomenon is lacking in the case of deep reservoirs due to late destruction. Here, we present fluid-inclusion assemblages from a deep reservoir in the Tazhong area of the Tarim Basin, northwestern China, which formed as a direct consequence of fluid pressure evolution. Based on thermodynamic measurements and simulations of the coexisting aqueous and petroleum inclusions in these assemblages, the history of petroleum activities was reconstructed. Our results show that all analyzed fluid-inclusion assemblages demonstrated variable pressure conditions in different charging stages, ranging from hydrostatic to overpressure (a pressure coefficient of up to 1.49). Sequential petroleum charging and partial oil cracking may have been the main contributors to overpressure. By comparing the phases of petroleum and fluid pressures in the two wells, ZS1 and ZS5, it can be inferred that overpressure inhibits oil cracking. Thus, overpressure exerts an important influence on the preservation of liquid hydrocarbon under high temperatures. Furthermore, our results reveal that the exploration potential for liquid petroleum is considerable in the deep reservoirs of the Tarim Basin.
On the Use of a Chloride or Fluoride Salt Fuel System in Advanced Molten Salt Reactors, Part 3; Radiation Damage
Authors: Noori-kalkhoran Omid , Jain Lakshay , Merk Bruno
Published: September 2024

Structural materials in fast reactors with harsh radiation environments due to high energy neutrons—compared to thermal reactors—potentially suffer from a higher degree of radiation damage. This radiation damage can change the thermophysical and mechanical properties of materials and, as a result, alter their performance and effective lifetime, in some cases leading to their disintegration. These phenomena can jeopardize the safety of fast reactors and thus need to be investigated. In this study, the effect of radiation damage on the vessels of molten salt fast reactors (MSFR) was evaluated based on two fundamental radiation damage parameters: displacement per atom (dpa) and primary knock-on atom (pka). Following the previous part of this article (Parts 1 and 2), an iMAGINE reactor core design (University of Liverpool, UK—chloride-based salt fuel system) and an EVOL reactor core design (CNRS, Grenoble, France, fluoride-based salt fuel system) with stainless steel and nickel-based alloy material vessels, respectively, were considered as case studies. The SPECTER and SPECTRA-PKA codes and a PTRAC card of MCNPX, integrated with a module which has been developed in MATLAB, named PTRIM and SRIM-2013 (using binary collision approximation), were employed individually to calculate and compare dpa and PKA (this master module containing all three tools has been appended to the iMAGINE-3BIC package for future use during reactor operations). Additionally, SRIM-2013 was applied in a 3D simulation of a radiation damage map on a small sample of vessels based on the calculated PKA. Our results showed a higher degree of radiation damage in the iMAGINE vessel compared to the EVOL one, which could be expected due to the harder neutron flux spectrum of the iMAGINE core compared to EVOL. In addition, the nickel alloy vessel showed better radiation damage resistance against high energy neutrons compared to the stainless steel one, although more investigations are required on thermal neutrons and alloy corrosion mechanisms to determine the best material for use in MSFR vessels.
Assessment of Green Hydrogen as Energy Supply Alternative for Isolated Power Systems and Microgrids
Authors: Barbosa Luciano T. , Vasconcelos Samuel D. , Rosas Pedro A. C. , Castro José F. C. , Barbosa Douglas C. P.
Published: September 2024

The energy supply for isolated systems remains a current challenge in Brazil and worldwide, particularly when known solutions are compared regarding their cost, ability to supply energy on demand, and sustainability. In this context, green hydrogen, which has been considered one of the main paths for the decarbonization of the energy chain, is also seen as a potential energy supply solution in isolated systems. Based on a literature review of articles addressing the topics of green hydrogen and isolated systems, an analysis is conducted on the application of green hydrogen as an energy supply solution for isolated systems. The review identified and collected data on important parameters for comparing solutions, such as CAPEX, OPEX, energy cost, and the price per kilogram of hydrogen. Using these data, analyses were performed to provide an overview of the application of green hydrogen in isolated systems. One of the objectives of this work is to present a comprehensive analysis of the green hydrogen chain and its application in the context of electricity generation, particularly for isolated systems in Brazil.
Screening and Engineering Yeast Transporters to Improve Cellobiose Fermentation by Recombinant Saccharomyces cerevisiae
Authors: Kretzer Leonardo G. , Knychala Marilia M. , da Silva Lucca C. , da Fontoura Isadora C. C. , Leandro Maria José , Fonseca César , Verstrepen Kevin J. , Stambuk Boris U.
Published: September 2024

Developing recombinant Saccharomyces cerevisiae strains capable of transporting and fermenting cellobiose directly is a promising strategy for second-generation ethanol production from lignocellulosic biomass. In this study, we cloned and expressed in the S. cerevisiae CEN.PK2-1C strain an intracellular β-glucosidase (SpBGL7) from Spathaspora passalidarum and co-expressed the cellobiose transporter SiHXT2.4 from Scheffersomyces illinoinensis, and two putative transporters, one from Candida tropicalis (CtCBT1 gene), and one from Meyerozyma guilliermondii (MgCBT2 gene). While all three transporters allowed cell growth on cellobiose, only the MgCBT2 permease allowed cellobiose fermentation, although cellobiose consumption was incomplete. The analysis of the β-glucosidase and transport activities revealed that the cells stopped consuming cellobiose due to a drop in the transport activity. Since ubiquitinylation of lysine residues at the N- or C-terminal domains of the permease are involved in the endocytosis and degradation of sugar transporters, we constructed truncated versions of the permease lacking lysine residues at the C-terminal domain (MgCBT2ΔC), and at both the C- and N-terminal domain (MgCBT2ΔNΔC) and co-expressed these permeases with the SpBGL7 β-glucosidase in an industrial strain. While the strain harboring the MgCBT2ΔC transporter continued to produce incomplete cellobiose fermentations as the wild-type MgCBT2 permease, the strain with the MgCBT2ΔNΔC permease was able to consume and ferment all the cellobiose present in the medium. Thus, our results highlight the importance of expressing cellobiose transporters lacking lysine at the N- and C-terminal domains for efficient cellobiose fermentation by recombinant S. cerevisiae.
Rheological Properties and Sensory Profile of Yoghurt Produced with Novel Combination of Probiotic Cultures
Authors: Ilić Nebojša , Belović Miona , Memiši Nurgin , Pestorić Mladenka , Škrobot Dubravka , Pezo Lato , Jevtić-Mučibabić Rada , Sanz Yolanda , Brouzes Jerome
Published: September 2024

Novel probiotic yoghurt was produced using the combination of bacterial cultures Lactobacillus plantarum HA119 and Bifidobacterium animalis subsp. lactis B94 and yoghurt bacteria Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Its basic nutritional composition, colour, texture, rheological properties, and sensory profile were compared with yoghurt produced using the same technological process and standard yoghurt cultures (control sample), as well as other commercially available yoghurts with different milk fat contents. Despite the fat content of the yoghurt made with the new probiotic cultures being 1.44%, its apparent viscosity was similar to that of high-fat yoghurt (2.99%). Other results from rheological measurements indicate that the new yoghurt had a stronger protein network, presumably due to the higher number of exopolysaccharides compared to both control and commercial yoghurts. Sensory analysis revealed that there were no statistically significant differences between the novel probiotic yoghurt and high-fat yoghurt as perceived by panellists. In conclusion, this combination of probiotic cultures can be used to produce yoghurt with rheological and sensory properties similar to high-fat yoghurts, without the need for hydrocolloids or changes in the production process.
Evaluation of Different Lactic Acid Bacteria as Starter Cultures for Nono—A West African Fermented Dairy Product
Authors: Ikele Onyeka M. , Ogu Chigoziri T. , Jiang Xiuping , Cavender George A.
Published: September 2024

Nono is a traditional cultured dairy product consumed across West Africa. In this study, five cultures isolated from Nigerian-produced nono and three purified lactic acid bacteria from the USDA-NRRL were examined for use in preparing nono starter cultures. Isolated cultures were characterized using microbiological and biochemical tests, including 16s rDNA sequencing to identify the genotype. Each isolated strain was cultured and inoculated into UHT milk (1% v/v) and allowed to ferment for 24 h at 25 °C. Fermented products were evaluated for pH, moisture content, water activity, and viscosity, and their descriptive sensory properties were noted. The isolate that resulted in sensory properties most similar to traditional nono was then used as the primary strain for subsequent starter culture blends made with the NRRL cultures. These blends were used for the fermentation of nono and compared with commercial nono samples. Isolates obtained from nono were as follows: Lactobacillus fermentum, Lactobacillus paracasei, and, surprisingly, Lactobacillus rhamnosus, which has not been previously reported as a part of the nono microflora. There was no significant difference in the physical parameters of nono made from the individual indigenous isolates and a similar pattern was observed for the organisms from NRRL, except that their total titratable acidity and viscosities were significantly (p < 0.05) higher than those of the indigenous organisms. Compounded starter made with L. rhamnosus and NRRL cultures was then used to make nono that showed significantly (p < 0.05) different pH and viscosity values than commercially purchased nono, while sensory evaluation showed that nono made from the new starter culture had a high overall consumer acceptance score.
Effect of Fiber Characteristics on the Structure and Properties of Quartz Fiber Felt Reinforced Silica-Polybenzoxazine Aerogel Composites
Authors: Liu Lanfang , Li Liangjun , Hu Yijie , Feng Junzong , Jiang Yonggang , Feng Jian
Published: September 2024

Fiber-reinforced aerogel composites are widely used for thermal protection. The properties of the fibers play a critical role in determining the structure and properties of the final aerogel composite. However, the effects of the fiber’s characteristics on the structure and properties of the aerogel composite have rarely been studied. Herein, we prepared quartz fiber felt-reinforced silica-polybenzoxazine aerogel composite (QF/PBSAs) with different fiber diameters using a simple copolymerization process with the ambient pressure drying method. The reasons for the effects of fiber diameter on the structure and properties of the aerogel composites were investigated. The results showed that the pore structure of the aerogel composites was affected by the fiber diameter, which led to significant changes in the mechanical behavior and thermal insulation performance. At room temperature, pore structure and density were found to be the main factors influencing the thermal conductivity of the composites. At elevated temperatures, the radiative thermal conductivity (λr) plays a dominant role, and reducing the fiber diameter suppressed λr, thus decreasing the thermal conductivity. When the QF/PBSAs were exposed to a 1200 °C butane flame, the PBS aerogel was pyrolyzed, and the pyrolysis gas carried away a large amount of heat and formed a thermal barrier in the interfacial layer, at which time λr and the pyrolysis of the PBS aerogel jointly determined the backside temperature of the composites. The results of this study can provide valuable guidance for the application of polybenzoxazine aerogel composites in the field of thermal protection.
Enhancing Operating Room Efficiency: The Impact of Computational Algorithms on Surgical Scheduling and Team Dynamics
Authors: Vladu Adriana , Ghitea Timea Claudia , Daina Lucia Georgeta , Țîrț Dorel Petru , Daina Mădălina Diana
Published: September 2024

Background/Objectives: Operating room (OR) efficiency is a critical factor in healthcare delivery, influenced by various components including surgical duration, room turnover, staff availability, and equipment preparation. Optimizing surgical start times through systematic strategies such as computational algorithms can significantly enhance OR utilization and management. This study aimed to improve OR efficiency by effectively managing and optimizing surgical start times using a computational algorithm designed to allocate resources more efficiently. Methods: A comparative analysis was conducted over two six-month periods from January to June 2023 and January to June 2024, with an improvement phase implemented between July and December 2023. Results: These measures encompassed training surgical personnel, strengthening the medical team, revising work procedures and hospital regulations, and the integration of a computational algorithm to better schedule and manage surgical interventions. Following the implementation of these comprehensive measures, enhanced management of surgical start times was observed. A statistically significant increase in surgical interventions between 8 and 10 a.m. was noted for the entire OR, rising from 28.65% to 32.13%. While OR occupancy between 8 and 12 a.m. increased from 63.91% to 73.30%, this difference was not statistically significant. However, a notable improvement in average occupancy between 9 and 11 a.m. was observed, rising from 87.53% to 98.07%. Conclusions: The introduction of computational algorithms significantly improved operating room efficiency, particularly in managing surgical start times. Additionally, team coordination improved as a result of more structured scheduling processes. The results indicate that effective management of surgical start times, especially when enhanced by computational algorithms, can positively impact OR utilization, particularly within specific time blocks.
Quantifying Physical Activity and Sedentary Behavior in Adults with Intellectual Disability: A Scoping Review of Assessment Methodologies
Authors: Firkin Cora J. , Obrusnikova Iva , Koch Laura C.
Published: September 2024

Background/Objectives: Methodologies for assessing behavior form the foundation of health promotion and disease prevention. Physical activity (PA) and sedentary behavior (SB) assessment methodologies have predominantly been developed for adults without an intellectual disability (ID), raising credibility concerns for adults with ID. The purpose was to synthesize the current state of assessment methodologies for quantifying PA and SB volume in the free-living setting for adults with an ID. Methods: Following PRISMA guidelines, eleven databases were searched through December 2023, yielding 8174 records. Data were extracted in Covidence (v.2.0), obtaining quantified PA and SB volume and assessment methodology characteristics across data collection and analysis, including tool(s) and technique(s) used, preparatory actions taken, instructions provided, and behavioral strategies employed during data collection. Results: Of the 8174 articles screened, 91 met the inclusion criteria. Common metrics included minutes/hours per day/week and steps per day/week. Despite 80% of the studies using objective techniques, substantial variation existed across studies regarding wearable models, sampling frequency and epoch length settings, calibration protocols, wearable placements, and data processing techniques. Limited studies provided instructions that did not exclusively rely on spoken language. Behavioral strategies varied, including self-monitoring, providing assistance or supervision, administering questionnaires verbally, issuing reminders, and offering monetary incentives. Conclusions: This review underscores the need for greater consistency and accessibility in PA and SB assessment methodology for adults with ID. Tailored preparation, instruction, and behavioral strategies may enhance assessment viability and suitability for adults with ID, with or without caregiver or researcher involvement in the free-living setting.
Current Understanding of Cardiovascular Calcification in Patients with Chronic Kidney Disease
Authors: Chen Sijie , Tang Rining , Liu Bicheng
Published: September 2024

The burden of chronic kidney disease (CKD) is increasing, posing a serious threat to human health. Cardiovascular calcification (CVC) is one of the most common manifestations of CKD, which significantly influences the morbidity and mortality of patients. The manifestation of CVC is an unusual accumulation of mineral substances containing calcium and phosphate. The main component is hydroxyapatite. Many cells are involved in this process, such as smooth muscle cells (SMCs) and endothelial cells. CVC is an osteogenic process initiated by complex mechanisms such as metabolic disorders of calcium and phosphorus minerals, inflammation, extracellular vesicles, autophagy, and micro-RNAs with a variety of signaling pathways like Notch, STAT, and JAK. Although drug therapy and dialysis technology continue to advance, the survival time and quality of life of CVC patients still face challenges. Therefore, early diagnosis and prevention of CKD-related CVC, reducing its mortality rate, and improving patients’ quality of life have become urgent issues in the field of public health. In this review, we try to summarize the state-of-the-art understanding of the progression of CVC and hope that it will help in the prevention and treatment of CVC in CKD.
Influence of Intraocular Pressure on the Expression and Activity of Sodium–Potassium Pumps in the Corneal Endothelium
Authors: Anney Princia , Charpentier Pascale , Proulx Stéphanie
Published: September 2024

The corneal endothelium is responsible for pumping fluid out of the stroma in order to maintain corneal transparency, which depends in part on the expression and activity of sodium–potassium pumps. In this study, we evaluated how physiologic pressure and flow influence transcription, protein expression, and activity of Na+/K+-ATPase. Native and engineered corneal endothelia were cultured in a bioreactor in the presence of pressure and flow (hydrodynamic culture condition) or in a Petri dish (static culture condition). Transcription of ATP1A1 was assessed using qPCR, the expression of the α1 subunit of Na+/K+-ATPase was measured using Western blots and ELISA assays, and Na+/K+-ATPase activity was evaluated using an ATPase assay in the presence of ouabain. Results show that physiologic pressure and flow increase the transcription and the protein expression of Na+/K+-ATPase α1 in engineered corneal endothelia, while they remain stable in native corneal endothelia. Interestingly, the activity of Na+/K+-ATPase was increased in the presence of physiologic pressure and flow in both native and engineered corneal endothelia. These findings highlight the role of the in vivo environment on the functionality of the corneal endothelium.
Synergistic Effect between the APOE ε4 Allele with Genetic Variants of GSK3B and MAPT: Differential Profile between Refractory Epilepsy and Alzheimer Disease
Authors: Toral-Rios Danira , Pichardo-Rojas Pavel , Ruiz-Sánchez Elizabeth , Rosas-Carrasco Óscar , Carvajal-García Rosa , Gálvez-Coutiño Dey Carol , Martínez-Rodríguez Nancy Lucero , Rubio-Chávez Ana Daniela , Alcántara-Flores Myr , López-Ramírez Arely , Martínez-Rosas Alma Rosa , Ruiz-Chow Ángel Alberto , Alonso-Vanegas Mario , Campos-Peña Victoria
Published: September 2024

Temporal Lobe Epilepsy (TLE) is a chronic neurological disorder characterized by recurrent focal seizures originating in the temporal lobe. Despite the variety of antiseizure drugs currently available to treat TLE, about 30% of cases continue to have seizures. The etiology of TLE is complex and multifactorial. Increasing evidence indicates that Alzheimer’s disease (AD) and drug-resistant TLE present common pathological features that may induce hyperexcitability, especially aberrant hyperphosphorylation of tau protein. Genetic polymorphic variants located in genes of the microtubule-associated protein tau (MAPT) and glycogen synthase kinase-3β (GSK3B) have been associated with the risk of developing AD. The APOE ε4 allele is a major genetic risk factor for AD. Likewise, a gene-dose-dependent effect of ε4 seems to influence TLE. The present study aimed to investigate whether the APOE ɛ4 allele and genetic variants located in the MAPT and GSK3B genes are associated with the risk of developing AD and drug-resistant TLE in a cohort of the Mexican population. A significant association with the APOE ε4 allele was observed in patients with AD and TLE. Additional genetic interactions were identified between this allele and variants of the MAPT and GSK3B genes.
The Interplay between Oxidative Stress and Sphingolipid Metabolism in Endometrial Cancer
Authors: Błachnio-Zabielska Agnieszka U. , Sadowska Patrycja , Zdrodowski Michał , Laudański Piotr , Szamatowicz Jacek , Kuźmicki Mariusz
Published: September 2024

Endometrial cancer is one of the most common malignancies in women. Sphingolipids, a group of lipids, play a key role in cancer biology. Cancer cells often exhibit abnormal redox homeostasis characterized by elevated levels of reactive oxygen species (ROS). Emerging evidence suggests that ceramides are involved in inhibiting proliferation and inducing apoptosis through ROS production. However, there is no data on the relationship between sphingolipid metabolism and oxidative status in endometrial cancer. The present study aims to assess the content of individual sphingolipids and oxidative status in healthy women and those with endometrial cancer. Sphingolipid analysis was performed using mass spectrometry. Total oxidative status (TOS) and total antioxidant capacity (TAC) were assessed colorimetrically. Our results showed a significant increase in the levels of all measured sphingolipids in cancer tissues compared to healthy endometrium. Additionally, a significant decrease in the S1P/ceramide ratio (sphingolipid rheostat) was observed in cancer patients, particularly for C14:0-Cer, C16:0-Cer, C18:1-Cer, C22:0-Cer, and C24:0-Cer. Furthermore, increased TOS and decreased TAC were found in cancer patients compared to healthy women. Significant correlations were observed between the levels of individual sphingolipids and oxidative status, with the strongest correlation noted between C22:0-Cer and TOS (r = 0.64). We conclude that endometrial cancer is characterized by profound changes in sphingolipid metabolism, contributing to oxidative dysregulation and tumor progression.
Lactoferrin Supplementation in Preventing and Protecting from SARS-CoV-2 Infection: Is There Any Role in General and Special Populations? An Updated Review of Literature
Authors: Manzoni Paolo , Messina Alessandro , Germano Chiara , Picone Simonetta , Masturzo Bianca , Sainaghi Pier Paolo , Sola Daniele , Rizzi Manuela
Published: September 2024

At the beginning of the pandemic, SARS-CoV-2 infection represented a great medical burden worldwide, as targeted and effective therapeutic options were lacking. This resulted in the revival of existing molecules and the increasing popularity of over-the-counter nutritional supplements. Among the latter, lactoferrin has been investigated as an adjuvant in COVID-19 therapy with conflicting results, mainly depending on different study designs. Considering that lactoferrin is one of the main components of human breast milk with anti-microbial and anti-inflammatory activity, it is conceivable that such bioactive molecule could be effective in supporting anti-SARS-CoV-2 infection therapy, especially in infants and pregnant women, two subpopulations that have been poorly evaluated in the existing clinical trials. This narrative review is intended to offer insight into the existing literature on lactoferrin’s biological functions and protective effects against COVID-19, with a special focus on pregnant women and their infants.
Preparation and Photodegradation of TiO2 Thin Films on the Inner Wall of Quartz Tubes
Authors: Chen Wei , Yan Qi , Yang Junjiao
Published: September 2024

Titanium dioxide thin films on the inner wall of quartz tubes were prepared in situ by the sol–gel method. Meanwhile, copper and cerium were loaded onto the surface of the titanium dioxide thin films to enhance photocatalytic activity and broaden the range of light absorption. X-ray diffractometer, X-ray photoelectron spectroscopy, scanning electron microscopy, energy dispersive X-ray spectrum, N2 gas adsorption, UV diffuse reflectance spectroscopy, electron paramagnetic resonance, photoluminescene spectroscopy, and so on were used to characterize the structure, morphology, chemical composition, and optical properties of the prepared photocatalyst. Methylene blue (MB) was used as a simulated organic pollutant to study the photocatalytic performance of the photocatalyst, which was a translucent, structurally stable, and reusable high-efficiency photocatalytic catalyst. Under UV lamp irradiation, the MB photodegradation efficiency was 94.5%, which reached 91.2% after multiple cycles.
Computational Study of Molecular Mechanism for the Involvement of Human Serum Albumin in the Renin–Angiotensin–Aldosterone System
Authors: Belinskaia Daria A. , Shestakova Natalia N. , Samodurova Kamila V. , Goncharov Nikolay V.
Published: September 2024

Human serum albumin (HSA) is an endogenous inhibitor of angiotensin I-converting enzyme (ACE) and, thus, plays a key role in the renin–angiotensin–aldosterone system (RAAS). However, little is known about the mechanism of interaction between these proteins, and the structure of the HSA–ACE complex has not yet been obtained experimentally. The purpose of the presented work is to apply computer modeling methods to study the interaction of HSA with ACE in order to obtain preliminary details about the mechanism of their interaction. Ten possible HSA–ACE complexes were obtained by the procedure of macromolecular docking. Based on the number of steric and polar contacts between the proteins, three leading complexes were selected, the stabilities of which were then tested by molecular dynamics (MD) simulation. Based on the results of MD simulation, the two most probable conformations of the HSA–ACE complex were selected. The analysis of these conformations revealed that the processes of oxidation of the thiol group of Cys34 of HSA and the binding of albumin to ACE can reciprocally affect each other. Known point mutations in the albumin molecules Glu82Lys, Arg114Gly, Glu505Lys, Glu565Lys and Lys573Glu can also affect the interaction with ACE. According to the result of MD simulation, the known ACE mutations, albeit associated with various diseases, do not affect the HSA–ACE interaction. A comparative analysis was performed of the resulting HSA–ACE complexes with those obtained by AlphaFold 3 as well as with the crystal structure of the HSA and the neonatal Fc receptor (FcRn) complex. It was found that domains DI and DIII of albumin are involved in binding both ACE and FcRn. The obtained results of molecular modeling outline the direction for further study of the mechanisms of HSA–ACE interaction in vitro. Information about these mechanisms will help in the design and improvement of pharmacotherapy aimed at modulation of the physiological activity of ACE.