bluuebunny/crossref_metadata_2025 huggingface dataset:
Cardiovascular Toxicity Related to Cancer Treatment: A Pragmatic Approach to the American and European Cardio‐Oncology Guidelines
Authors: Alexandre Joachim , Cautela Jennifer , Ederhy Stéphane , Damaj Ghandi Laurent , Salem Joe‐Elie , Barlesi Fabrice , Farnault Laure , Charbonnier Aude , Mirabel Mariana , Champiat Stéphane , Cohen‐Solal Alain , Cohen Ariel , Dolladille Charles , Thuny Franck
Published: September 2020

The considerable progress made in the field of cancer treatment has led to a dramatic improvement in the prognosis of patients with cancer. However, toxicities resulting from these treatments represent a cost that can be harmful to short‐ and long‐term outcomes. Adverse events affecting the cardiovascular system are one of the greatest challenges in the overall management of patients with cancer, as they can compromise the success of the optimal treatment against the tumor. Such adverse events are associated not only with older chemotherapy drugs such as anthracyclines but also with many targeted therapies and immunotherapies. Recognizing this concern, several American and European governing societies in oncology and cardiology have published guidelines on the cardiovascular monitoring of patients receiving potentially cardiotoxic cancer therapies, as well as on the management of cardiovascular toxicities. However, the low level of evidence supporting these guidelines has led to numerous discrepancies, leaving clinicians without a consensus strategy to apply. A cardio‐oncology expert panel from the French Working Group of Cardio‐Oncology has undertaken an ambitious effort to analyze and harmonize the most recent American and European guidelines to propose roadmaps and decision algorithms that would be easy for clinicians to use in their daily practice. In this statement, the experts addressed the cardiovascular monitoring strategies for the cancer drugs associated with the highest risk of cardiovascular toxicities, as well as the management of such toxicities.
Sociotechnical Transformative Effects of an ICT Project in Rural Bangladesh
Authors: Stillman Larry , Sarrica Mauro , Anwar Misita , Sarker Anindita , Farinosi Manuela
Published: September 2020

The purpose of this article is to provide lessons from the field about an Information and Communication Technology for Development (ICT4D) project (Participatory Research and Ownership With Technology, Information and Change [PROTIC]) concerned with the use of mobile phones by women in remote villages in Bangladesh. The Bangladeshi government considers that the role of ICT in social and economic transformation is significant for the country’s development. International nongovernmental organizations (NGOs) also regard ICT as important but are challenged as how to use them effectively for their programs and how to deal with long-term sustainability, digital divides, gender, and cultural issues. This article considers the PROTIC project as a modeling force for innovation and pressure on established sociotechnical structures. In this analysis, we follow what Donner defines as the “interrelationship” perspective, as applied to ICT4D. In particular, the notions of niche, regime, and landscape will be used to frame the changes that a village-level project may activate or respond to at the micro, meso, and macro levels of sociotechnical interaction. A mixed methods approach has been implemented during the 4 years of the project to monitor its outcomes, including interviews with project participants, reports of monthly consultations and training with villagers, extensive surveys, analysis of the Facebook profile of the project, and field notes and interviews with local NGOs and international NGO staff. Results show that the women villagers have undergone a transformation in attitudes, skills, and practices associated with mobile phone use. Transformations at individual and community niche levels have in turn influenced the conceptual framework of local and international NGOs and have also contributed to the reorientation of other regime actors, such as universities, major NGOs, and the government. Methodological constraints as well as the complexity of conducting international fieldwork with multiple actors will also be discussed.
Efficacy of Tiered Training on Paraeducator Implementation of Systematic Instructional Practices for Students With Severe Disabilities
Authors: Brock Matthew E. , Barczak Mary A. , Anderson Eric J. , Bordner-Williams Nichole M.
Published: September 2020

Existing approaches for training paraeducators rely heavily on intensive one-to-one coaching and may not be feasible in practice. In this study, we test a tiered training model in which all paraeducators first received group training, and then coaching was provided only for the subset who did not meet performance criteria after group training. Using a concurrent multiple-probe design staggered across classrooms, we demonstrated a functional relation between the tiered model and implementation fidelity of two systematic prompting strategies across 13 paraeducators in five schools. Nine paraeducators achieved the performance criterion for both practices with group training alone, and the remaining four met the criterion after teacher-delivered coaching. In addition, paraeducators generalized implementation to new situations, and students with severe disabilities who received instruction made progress on individualized goals. Based on these findings, a tiered training model is a feasible and promising means to train paraeducators.
Evaluation of Enoxaparin for Inpatient Venous Thromboembolism Prophylaxis in End-Stage Renal Disease Patients on Hemodialysis
Authors: Sacks Jamie , Luc Stanley A.
Published: September 2020

Background: Enoxaparin is not recommended for venous thromboembolism (VTE) prophylaxis in the end-stage renal disease (ESRD) on hemodialysis (HD) population due to concerns for drug accumulation and increased bleeding risk. Due to the paucity of literature with clinical outcomes to support this theoretical safety concern, the purpose of this study was to compare the risks of bleeding of enoxaparin and unfractionated heparin (UFH) in hospitalized, HD-dependent patients. Methods: This retrospective cohort study examined ESRD on HD patients who received either subcutaneous enoxaparin or UFH for VTE prophylaxis and were admitted for at least 48 hours. The primary outcome was major bleeding or clinically relevant non-major bleeding (CRNMB) as guided by definitions from the International Society of Thrombosis and Haemostasis. Results: A total of 322 enoxaparin and 10 UFH patients were analyzed. All enoxaparin patients were dosed 30 mg subcutaneous daily. Twenty-two (6.8%) enoxaparin and zero UFH patients experienced major or CRNMB ( P = .498). Three enoxaparin patients suffered fatal hemorrhages. Multiple logistic regression demonstrated thrombocytopenia was associated with bleeding (odds ratio 4.23, P = .004). Conclusion: The difference in major or CRNMB rates between both anticoagulants was not statistically significant. However, the 6.8% bleed rate is concerning for inpatient enoxaparin usage, and caution should be applied when considering this drug for VTE prophylaxis in the ESRD on HD population.
What Factors Predict the Self-Identification of Drug Dependency Among Australian Police Detainees? Prevalence, Correlates, and Implications for the Criminal Justice System
Authors: Langfield Cameron T. , Payne Jason L.
Published: September 2020

The drug-crime nexus has received interest from both drug-crime scholars and public policy experts internationally. While there is little disagreement that drug use is linked to higher rates of crime, causation remains hotly contested. One area of emerging interest is the confounding influence of “identity” in shaping long-term behavioral drug use and criminal trajectories. In this study, we explore the prevalence with which recent drug-using police detainees self-identify as drug-dependent and, using logistic regression, model self-identification as a function of one’s demographic, and drug-use profile. We find that being female and younger is associated with an increased odds of self-identifying oneself as dependent, controlling for drug use variables. Of the five drug types, primary heroin users are the most likely to self-identify, whereas cocaine, cannabis, and amphetamine users are less likely. To end, the potential implications of these results are discussed, and future research avenues are explored.
Introducing the Dynamic Destination Satisfaction Method: An Analytical Tool to Track Tourism Destination Satisfaction Trends with Repeated Cross-sectional Data
Authors: Guizzardi Andrea , Mariani Marcello M.
Published: September 2020

This study introduces a new method, named Dynamic Destination Satisfaction Method (DDSME), to model tourists’ satisfaction with a destination (and its attributes), breaking it down into an individual-level component (linked to the specific individual tourists’ perceptions) and a system-level (time-related) component (common to all the tourists). Moreover, this work develops a matrix “entropy/trend accuracy” that destination managers can use to understand to what extent managing a specific attribute has increased tourists’ satisfaction with the destination over multiyear time spans. We test the innovative method on a large data set, covering the period 1997-2015 and including almost 0.8 million observations. By doing so, we analyze tourists’ satisfaction with tourism-related sectors and attributes of Italy as an inbound tourism destination and we use the matrix to map out destination attributes over time. The findings indicate that courtesy, art, and food are strategic attributes to enhance satisfaction in the long term.
Customary Land-Tenure and the Poor: A Study of Jharkhand and Meghalaya
Authors: Goyal Ankita
Published: September 2020

For most people living in rural India having access and control over land is crucial for their livelihood, more so in the case of tribals. This article analyses the nature of the customary land-tenure system in some districts of Jharkhand and Meghalaya and their impact on livelihood patterns, food security and poverty. Based on both secondary and primary data, the article seeks to examine the nature of the customary land-tenure systems in selected scheduled areas; specifically identifying the status of locals versus non-locals in managing land resources and analysing the extent to which women have been able to secure land rights under customary laws. The article concludes that though there are both positive and negative aspects to community and individual ownership of agricultural land, but on the whole the prevailing system does not helping in bettering the conditions of disadvantaged communities.
Book review: Hem Borker, Madrasas and the Making of Islamic Womanhood
Authors: Jha Shefali
Published: September 2020

Hem Borker, Madrasas and the Making of Islamic Womanhood. New Delhi: Oxford University Press 2018, 326 pp., ₹1195, ISBN 9780199484225
Ashoke Kumar Sarkar and Abdus Samad Gayen (Eds.), Karl Marx, Bicentennial (1818–2018) Lectures
Authors: Verma Vidhu
Published: September 2020

Ashoke Kumar Sarkar and Abdus Samad Gayen (Eds.), Karl Marx, Bicentennial (1818–2018) Lectures. Kolkata: Seribaan, 2020, ₹90, ISBN: 978818749266
Book review: Rama V. Baru and Madhurima Nundy, Commercialisation of Medical Care in China: Changing Landscapes
Authors: Prasad Vandana
Published: September 2020

Rama V. Baru and Madhurima Nundy, Commercialisation of Medical Care in China: Changing Landscapes. India: Routledge, 2019, 130 pp, ₹695, ISBN 978-0-367-4483-9 https://doi.org/10.4324/9780429341243
Book review: Chinmay Tumbe, India Moving: A History of Migration
Authors: Singh Shubha
Published: September 2020

Chinmay Tumbe, India Moving: A History of Migration, New York: Penguin Viking, 2018, ₹444, 304 pp, ISBN-13: 978-0670089833.
Tenant Activism and the Demise of Urban Renewal: Tenants, Governance, and the Struggle for Recognition at Habitations Jeanne-Mance in Montreal
Authors: Nettling Pierson
Published: September 2020

How public housing tenant activism altered Canadian housing policy in the 1960s continues to be underresearched within urban renewal, Canadian Left, or North American housing studies. Organizing against local governance and the federal rental contract, their nationwide activism directly led to the Hellyer Report, which officially ended urban renewal in 1969. Situated within this period are the tenants of Habitations Jeanne-Mance (HJM) in Montreal. Contrasting the prevailing view of Canadian tenants being politically isolated, HJM tenants led movements against the rental contract, demanded tenant representation, and sought social rights for low-income people within the Montreal Left. As more representative of the Canadian experience in the 1960s, HJM demonstrates how tenant activism has shaped the history of housing in Canada.
Freight and Service Parking Needs in Historical Centers: A Case Study in São João Del Rei, Brazil
Authors: Silva Kaique , da Silva Lima Renato , Alves Roberta , Yushimito Wilfredo F. , Holguín-Veras José
Published: September 2020

The objective of this study is to analyze the demand for loading and unloading parking spaces in the center of São João Del Rei, a historical city in the State of Minas Gerais, Brazil, through freight trip generation models. To generate the models, the number of employees is used as an independent variable. Results show that the historical center receives an average of 710 freight trips per day, which would require at least 43 spaces for loading and unloading. As the center has only eight such spaces available, representing 18% of total demand, this study proposes new locations and suggests transportation demand management measures that could be used in conjunction with the allocation of new parking spots.
Are the Wages Paid by Non-Profits Sensitive to Volunteering? Evidence From France
Authors: Prouteau Lionel , Tchernonog Viviane
Published: September 2020

Volunteers and paid staff frequently work alongside each other in nonprofit organizations. Nevertheless, the possible impact of volunteering on the wages of the paid staff has hardly ever been investigated due to the scarcity of data suited to such research. Based on the matching of two French databases and using several indicators relating to volunteering, this article examines the relationship between volunteering and wages by carrying out investigations that differentiate between employees depending on their position in the socio-occupational hierarchy and the field of activity of their organizations. The results confirm the value of such an approach based on disaggregated data by revealing the diversity of situations. This diversity ranges from some cases where no relationship is observed between volunteering and wages to others where there is a negative relationship and even in a few cases, a positive one.
The Normativity of Work: Lockean and Marxist Overlapping Consensus on Just Work
Authors: Kwok Chi
Published: September 2020

Work is an integral part of modern society. However, the question of the normative conditions that distinguish just from unjust work has been under-investigated in political theory. This article, by comparing the Lockean and Marxist views of just work, aims to show that a set of overlapping normative requirements of just work can be derived from them despite their polarized economic and political views. Locke has been appropriated by contemporary libertarians as the central figure of right-based libertarian market economy while Marx has been seen by the left as a central intellectual resource to criticize capitalism’s tendency for degrading work. Given this usual polarization, the article can shed light on a common set of normative requirements concerning just work between them. This overlapping set of normative requirements of just work invites reflections on possible common grounds of just work between the contemporary right and the left who have appropriated the two thinkers as their foundational theoretical building blocks.
It’s in Me to Give: Canadian Gay, Bisexual, and Queer Men’s Willingness to Donate Blood If Eligible Despite Feelings of Policy Discrimination
Authors: Grace Daniel , Gaspar Mark , Klassen Benjamin , Lessard David , Brennan David J. , Lachowsky Nathan J. , Adam Barry D. , Cox Joseph , Lambert Gilles , Anand Praney , Jollimore Jody , Moore David , Hart Trevor A.
Published: September 2020

Blood donation policies governing men who have sex with men have shifted significantly over time in Canada—from an initial lifetime ban in the wake of the AIDS crisis to successive phases of time-based deferment requiring periods of sexual abstinence (5 years to 1 year to 3 months). We interviewed 39 HIV-negative gay, bisexual, queer, and other sexual minority men (GBM) in Vancouver, Toronto, and Montreal to understand their willingness to donate blood if eligible. Transcripts were coded following inductive thematic analysis. We found interrelated and competing expressions of biological and sexual citizenship. Most participants said they were “safe”/“low risk” and “willing” donors and would gain satisfaction and civic pride from donation. Conversely, a smaller group neither prioritized the collectivizing biological citizenship goals associated with expanding blood donation access nor saw this as part of sexual citizenship priorities. Considerable repair work is required by Canada’s blood operators to build trust with diverse GBM communities.
Vibration characteristics of multiple functionally graded nonuniform beams
Authors: Sari Ma’en S , Al-Dahidi Sameer
Published: September 2020

Based on the Euler–Bernoulli beam theory, the natural vibration behavior of functionally graded nonuniform multiple beams has been investigated. It is assumed that the beams are joined by elastic translational springs, and the properties of the beams vary along the axial direction. The Chebyshev spectral collocation method has been used to convert the governing differential equations of transverse motion into a system of algebraic equations that are put in the matrix–vector form. Then, the dimensionless transverse frequencies are obtained by solving the eigenvalue problem. The influence of several factors, such as the stiffness parameters of the coupling translational springs, the properties of the cross section of the beams, and the boundary conditions on the frequencies, has been carried out. The results generated from the Chebyshev spectral collocation method have been verified by comparing them with those reported in other studies and references from the literature. Several numerical examples have been presented and discussed to analyze the system under consideration. The authors hope that the findings of the current study are helpful in designing and characterizing multiple nonuniform thin engineering structures.
Building Educational Resilience Through Transnational Mobility Trajectories: Young People Between Ghana and The Netherlands
Authors: van Geel Joan , Mazzucato Valentina
Published: September 2020

This study investigates how young Ghanaians’ mobility between Ghana and The Netherlands relates to their educational resilience. Based on 20 months of multi-sited ethnographic research following 30 youths of 16–25 age group, we deploy a socio-ecological approach developed in social psychology to identify three resilience-building mechanisms: connection to motivational others, active recollection and comparative confrontation. These mechanisms have to date remained outside of the purview of resilience research and research on migration and education, as these fields focus on the nation-state rather than the transnational context in which young people operate. They thereby ignore mobility patterns that make other contexts relevant to young people’s educational resilience. As such, we expand the socio-ecological model of resilience to include transnational elements and show how mobility can positively relate to education and the resilience of migrant youth.
Existential advertising in late modernity: Meaningful work in higher education advertisements
Authors: Cannizzo Fabian , James Sara
Published: September 2020

The ongoing massification of higher education has entailed a changing relationship between higher education providers and students. While scholars have been quick to identify the political implications of the quasi-market model for the student-customer, there has been significantly less focus on the role that advertising plays in facilitating a student-consumer culture. This article uses an analysis of advertisements directed towards potential domestic university students in Australia to explore how the idea of a university ‘experience’ and meaningful work are discursively constructed within late modernity. Many advertisements draw on existential themes of living a meaningful, authentic or exceptional life and finding a fulfilling career, mythologising the role of the university within an idealised social order. This study highlights the need to understand the economic value of higher education as embedded in cultural economy, as well as the value of this framework for researching student experiences and marketing.
From evidence to practice: Developing best practice guidelines for the delivery of activities to people living with moderate to advanced dementia using a pragmatic observational study
Authors: Burke Amanda , Jones Andy , Hughes Ryan , Player Emily
Published: September 2020

The benefits of physical activities for those living with moderate to advanced dementia are well documented and include improved well-being and quality of life. What is less well known is how best to deliver such activities to make them meaningful for those taking part and, more generally, how to develop good practice guidance for working with this group. This article reports on an observational study of a physical activity programme in a residential care setting, Mobile Me, and on the process used to develop good practice guidance from it, which included input from a range of stakeholders. Learnings from this study conclude that changes in delivery and setting can contribute to a difference in the quality of the experience for participants and their levels of well-being during sessions. The findings from the study were consolidated into four themes for disseminating best practice: promoting the right atmosphere, environment, communication, and adaptations. These form part of a new multimedia best practice guide for delivering physical activities to those living with moderate to advanced dementia.
Pedagogic Tailoring of a Human Research Ethics Training for Community-Engaged Research With Latinos
Authors: Documet Patricia I. , Louth William , Smith-Tapia Ivonne , Jaime Maria Catrina , Miller Elizabeth , Taverno Ross Sharon E.
Published: September 2020

Because of the increase in community-engaged research, several human research ethics trainings for laypeople have been developed. We aim to (1) describe the pedagogical tailoring of a research ethics training for laypeople for a research study where promotores—community health workers—delivered an intervention to increase health care access and promote healthy behaviors among Latinos and (2) present results of the application of the training after 4 months in the field. We tailored a previously developed training to Latino community members implementing a research study. Key modifications included (1) translation (2) use of pedagogical tools, such as cooperative learning, role-plays, and inclusion of cultural preferences. One novel addition was to use dialogues that the trainees enacted and then discussed. We evaluated the training with a posttraining survey with eight community liaisons and 13 promotores implementing the intervention, and a focus group with eight promotores, 4 months after working in the field. Trainees said they felt confident obtaining informed consent, felt the dialogues were realistic and helped them remember what they learned, and wanted more feedback from trainers on their performance. Promotores demonstrated the application of ethical principles beyond the training by discussing the possibility of advertising broadly in social media (justice), the risks and benefits of providing community resources to participants (beneficence), and the university’s role in legitimizing their position as promotores (respect). We conclude that a pedagogically tailored ethics research training for laypeople can be successful and that dialogues to be enacted need to be explored further.
Interrogating LeftTube: ContraPoints and the Possibilities of Critical Media Praxis on YouTube
Authors: Maddox Jessica , Creech Brian
Published: September 2020

For the past several years, media commentary and cultural analysis has grown increasingly fixated on YouTube as a radicalization hub, particularly around extremist, alt-right content. However, a growing community of leftist YouTube content creators, loosely coalescing into the platform’s “LeftTube,” have developed dialogic relationships with some of YouTube’s most extreme content. This work focuses on one specific LeftTube creator, ContraPoints, to explore how those on the political left engage with YouTube’s cultural and technical affordances to challenge alt-right ideology. Through a textual analysis of ContraPoints’ top thirty videos, we identified three main discursive strategies: practicing deradicalization strategies on YouTube; establishing alt-right individuals as an intentional audience; and developing a language for escaping alt-right logics. ContraPoints, and her rightful critics, demonstrate how political subjectivities are created and contested within YouTube as both a technical and cultural space.
WITHDRAWN: Braiding as a Feminist Methodology: Tensions and Possibilities of Weaving Together
Authors: Ward Amber
Published: September 2020

Ward, A. (2020). Braiding as a Feminist Methodology: Tensions and Possibilities of Weaving Together. Cultural Studies ↔ Critical Methodologies, 21(1), 3–15. https://doi.org/10.1177/1532708620953200.

This article was withdrawn after publication.
Schema Therapy for Chronic Depression Associated with Childhood Trauma: A Case Study
Authors: Herts Kate L. , Evans Susan
Published: September 2020

Chronic depression lasting longer than 2 years is often undertreated. Research suggests that maladaptive cognitive schemas mediate the association between childhood trauma and later depression. Schema Therapy (ST) is an integrative treatment approach that targets maladaptive cognitive schemas through cognitive, behavioral, interpersonal, and experiential interventions. ST has been studied in patients with chronic depression with good results. The purpose of this case study is to detail how an ST treatment was used to treat chronic depression in a woman, “Amy,” with a childhood trauma history. Amy presented with a persistent depressive disorder that had lasted over 40 years. An ST approach was chosen in light of the chronicity of Amy’s symptoms and her childhood trauma history. Standardized measures including the Beck Depression Inventory were used to assess progress throughout treatment. We provide a comprehensive summary of the 22-session ST case conceptualization and treatment, through which Amy’s depressive symptoms evidenced a 73 percent reduction. Amy qualitatively reported reduced depressive rumination and avoidance behaviors as well as increased frequency of positive mood.
Developing an Empathy Spectrum for Games
Authors: Jerrett Adam , Howell Peter , Dansey Neil
Published: September 2020

Games often encourage players to feel empathy for characters or scenarios by design. However, the term ‘empathy’ is often misunderstood and used in a variety of contexts as a substitute for feelings of sympathy, pity and compassion. This article defines a distinction between these similar terms and uses their definitions to describe how players emotionally engage with a game. This helps define an empathy spectrum, ranging from pity to compassion, that can be used to subjectively classify different games. To show the spectrum in use, the article discusses a variety of video games that can be placed at the spectrum’s key points, before discussing how games might reach the spectrum’s furthest point: compassion. The research hopes that modelling these abstract psychological concepts on this spectrum can help game designers, players and scholars better understand the range of emotional responses present in games.
Lifestyle Medicine Philippines: A Journey of Hope
Authors: Manez Johann Kim T.
Published: September 2020

From rock bottom beginnings, lifestyle medicine became a symbol of hope for people with chronic diseases in the Philippines. Noncommunicable diseases remain the burden of disease in the country and with the acceptance of lifestyle medicine by the Philippine Medical Association, the future of lifestyle medicine looks bright in this part of the world.
Bottle-Feeding Challenges in Preterm-Born Infants in the First 7 Months of Life
Authors: Hill Rebecca R. , Park Jinhee , Pados Britt F.
Published: September 2020

Preterm infants frequently experience oral feeding challenges while in the neonatal intensive care unit, with research focusing on infant feeding during this hospital stay. There is little data on symptoms of problematic feeding in preterm-born infants in the months after discharge. The purpose of this study was to describe symptoms of problematic bottle-feeding in the first 7 months of life in infants born preterm, compared to full-term infants. Parents of infants less than 7 months old completed an online survey that included the Neonatal Eating Assessment Tool—Bottle-feeding and questions about the infant’s medical and feeding history. General linear models were used to evaluate differences in NeoEAT—Bottle-feeding total score and subscale scores by preterm category, considering other significant factors. Very preterm infants had more symptoms of problematic bottle-feeding than other infants. Current age, presence of gastroesophageal reflux, and anomalies of the face/mouth were associated with problematic bottle-feeding.
Markers of vitality in ovaries of transmen after long-term androgen treatment: a prospective cohort study
Authors: Marschalek Julian , Pietrowski Detlef , Dekan Sabine , Marschalek Marie-Louise , Brandstetter Maximilian , Ott Johannes
Published: September 2020

Background

Gender-affirming hormone therapy has been hypothesized to reduce the patient’s reproductive potential in transmen, although the exact long-term effects on future fertility are unknown.

Methods

In this prospective cohort study we aimed to evaluate ovaries of 20 transmen by using hormone serum levels, histomorphological analysis and fluorescence activated cells sorting (FACS) analysis – in order to assess the amount of vital cells.

Results

The median total number of follicles per field of view was 39 (IQR 12–122). Of all follicles (n = 1661), the vast majority was primordial (n = 1505, 90.6%), followed by primary (n = 76, 4.6%), abnormal (n = 63, 3.8%) and secondary follicles (n = 17, 1.0%). FACS analysis was available for 13 samples (65.0%) and the median frequency of vital cells was 87.5% (IQR, 77.7–95.4%). Both a higher age (p = 0.032) and a lower BMI (p = 0.003) were significantly associated with a higher frequency of vital cells.

Conclusion

The majority of ovarian cells after long-term androgen treatment were vital in FACS analysis and histomorphological evaluation revealed a normal cortical follicle distribution. These results are currently exploratory, but might be promising for issues on fertility preservation.

Trial registration

The study was approved by the ethics committee of the Medical University of Vienna (EK 2240/2016) and was retrospectively registered in the Current Controlled Trials Register (registration number NCT03649087, date of registration: 28.08.2018).
Chloride intracellular channel 4 participate in the protective effect of Ginkgolide B in MPP+ injured MN9D cells: insight from proteomic analysis
Authors: Feng Zili , Zhu Zhibin , Chen Wang , Bai Yu , Hu Daihua , Cheng Jia
Published: September 2020

Background

Ginkgolide B (GB), the extract of G. biloba leaves, has been shown to be protective against many neurological disorders, including Parkinson’s disease (PD). Efforts have been made to synthesized ginkgolides analogs and derivatives with more targeted and smaller molecular weight. In the present study, four GB derivatives (GBHC-1-GBHC-4) were synthesized, and their protective roles in N-methyl-4-phenylpyridinium (MPP +) injured MN9D dopaminergic neuronal cell line were evaluated. Also, cell response mechanisms upon these GB derivatives treatment were analyzed by iTRAQ proteomics.

Methods

MN9D cells were treated with MPP + to induce in vitro cell models of PD. Four GB derivatives (GBHC-1-GBHC-4) were synthesized, and their protective roles on cell viability and apoptosis in in vitro PD model cells were evaluated by CCK8 assay, fluorescence-activated cell sorting and DAPI staining, respectively. The proteomic profiles of MPP+ injured MN9D cells pretreated with or without GB and GB derivatives were detected using the isobaric tags for relative and absolute quantification (iTRAQ) labeling technique.

Results

Pretreatment with GBHC-1-GBHC-4 noticeably increased cell viability and attenuated cell apoptosis in MPP+ -injured MN9D cells. Using proteomic analysis, we identified differentially expressed proteins upon GB and GB derivatives treatment. Chloride intracellular channel 4 (CLIC4) and “protein processing in endoplasmic reticulum” pathways participated in the protective roles of GB and GBHC-4. GB and GBHC-4 pretreatment could significantly reverse MPP+ -induced CLIC4 expression and translocation from cytoplasm to nucleus of MN9D cells.

Conclusions

Quantitative comparative proteomic analysis identified differentially expressed proteins associated with GB and GB derivatives. We further verified the expression of CLIC4 by western blotting and immunocytochemistry assay. This bio-information on the identified pathways and differentially expressed proteins such as CLIC4 provide more targeted directions for the synthesis of more effective and targeted GB derivatives for the treatment of neurological disorders.
Remote cardiac rehabilitation is a good alternative of outpatient cardiac rehabilitation in the COVID-19 era
Authors: Nakayama Atsuko , Takayama Naoko , Kobayashi Momoko , Hyodo Kanako , Maeshima Naomi , Takayuki Fujiwara , Morita Hiroyuki , Komuro Issei
Published: September 2020

Background

In the wake of the coronavirus disease 2019 (COVID-19) pandemic, people need to practice social distancing in order to protect themselves from SARS-CoV-2 infection. In such stressful situations, remote cardiac rehabilitation (CR) might be a viable alternative to the outpatient CR program.

Methods

We prospectively investigated patients hospitalized for heart failure (HF) with a left ventricular ejection fraction of < 50%. As for patients who participated in the remote CR program, telephone support was provided by cardiologists and nurses who specialized in HF every 2 weeks after discharge. The emergency readmission rate within 30 days of discharge was compared among the outpatient CR, remote CR, and non-CR groups, and the EQ-5D score was compared between the outpatient CR and remote CR groups.

Results

The participation rate of HF patients in our remote CR program elevated during the COVID-19 pandemic. As observed in the outpatient CR group (n = 69), the emergency readmission rate within 30 days of discharge was lower in the remote CR group (n = 30) than in the non-CR group (n = 137) (P = 0.02). The EQ-5D score was higher in the remote CR group than in the outpatient CR group (P = 0.03) 30 days after discharge.

Conclusions

Remote CR is as effective as outpatient CR for improving the short-term prognosis of patients hospitalized for heart failure post-discharge. This suggests that the remote CR program can be provided as a good alternative to the outpatient CR program.
Genome-wide analysis of lncRNA and mRNA expression and endogenous hormone regulation during tension wood formation in Catalpa bungei
Authors: Xiao Yao , Yi Fei , Ling Juanjuan , Yang Guijuan , Lu Na , Jia Zirui , Wang Junchen , Zhao Kun , Wang Junhui , Ma Wenjun
Published: September 2020

Background

Phytohormones are the key factors regulating vascular development in plants, and they are also involved in tension wood (TW) formation. Although the theory of hormone distribution in TW formation is widely supported, the effects of endogenous hormones on TW formation have not yet been assessed. In this study, TW formation was induced in Catalpa bungei by artificial bending. The phytohormone content of TW, opposite wood (OW) and normal wood (NW) was determined using liquid chromatography-mass spectrometry (LC-MS), and transcriptome sequencing was performed. The hormone content and related gene expression data were comprehensively analyzed.

Results

The results of analyses of the plant hormone contents indicated significantly higher levels of cis-zeatin (cZ), indoleacetic acid (IAA) and abscisic acid (ABA) in TW than in OW. Genes involved in the IAA and ABA synthesis pathways, such as ALDH (evm.model.group5.1511) and UGT (evm.model.scaffold36.20), were significantly upregulated in TW. and the expression levels of ARF (evm.model.group5.1332), A-ARR (evm.model.group0.1600), and TCH4 (evm.model.group2.745), which participate in IAA, cZ and Brassinolide (BR) signal transduction, were significantly increased in TW. In particular, ARF expression may be regulated by long noncoding RNAs (lncRNAs) and the HD-ZIP transcription factor ATHB-15.

Conclusions

We constructed a multiple hormone-mediated network of C. bungei TW formation based on hormone levels and transcriptional expression profiles were identified during TW formation.
The combined utilization of Chlorhexidine and Voriconazole or Natamycin to combat Fusarium infections
Authors: Jiang Tao , Tang Jing , Wu Zhiqin , Sun Yi , Tan Jingwen , Yang Lianjuan
Published: September 2020

Background

Fusarium species are the fungal pathogens most commonly responsible for the mycotic keratitis, which are resistant to the majority of currently available antifungal agents. The present study was designed to assess the efficacy of a combination of low doses chlorhexidine with two other commonly used drugs (voriconazole and natamycin) to treat Fusarium infections.

Results

We utilized combinations of chlorhexidine and natamycin or voriconazole against 20 clinical Fusarium strains in vitro using a checkerboard-based microdilution strategy. In order to more fully understand the synergistic interactions between voriconazole and chlorhexidine, we utilized a Galleria mellonella model to confirm the combined antifungal efficacy of chlorhexidine and voriconazole in vivo. We found that for voriconazole, natamycin, and chlorhexidine as single agents, the minimum inhibitory concentration (MIC) ranges were 2–8, 4–16, and > 16 μg/ml, respectively. In contrast, the MIC values for voriconazole and chlorhexidine were reduced to 0.25–1 and 1–2 μg/ml, respectively, when these agents were administered in combination, with synergy being observed for 90% of tested Fusarium strains. Combined chlorhexidine and natamycin treatment, in contrast, exhibited synergistic activity for only 10% of tested Fusarium strains. We observed no evidence of antagonism. Our in vivo model results further confirmed the synergistic antifungal activity of chlorhexidine and voriconazole.

Conclusions

Our results offer novel evidence that voriconazole and chlorhexidine exhibit synergistic activity when used to suppress the growth of Fusarium spp., and these agents may thus offer value as a combination topical antifungal treatment strategy.
Genome-wide identification and characterization of GRAS genes in soybean (Glycine max)
Authors: Wang Liang , Ding Xianlong , Gao Yingqi , Yang Shouping
Published: September 2020

Background

GRAS proteins are crucial transcription factors, which are plant-specific and participate in various plant biological processes. Thanks to the rapid progress of the whole genome sequencing technologies, theGRASgene families in different plants have been broadly explored and studied. However, comprehensive research on the soybean (Glycine max)GRASgene family is relatively lagging.

Results

In this study, 117Glycine maxGRASgenes (GmGRAS) were identified. Further phylogenetic analyses showed that theGmGRASgenes could be categorized into nine gene subfamilies: DELLA, HAM, LAS, LISCL, PAT1, SCL3, SCL4/7, SCR and SHR. Gene structure analyses turned out that theGmGRASgenes lacked introns and were relatively conserved. Conserved domains and motif patterns of theGmGRASmembers in the same subfamily or clade exhibited similarities. Notably, the expansion of theGmGRASgene family was driven both by gene tandem and segmental duplication events. Whereas, segmental duplications took the major role in generating newGmGRASgenes. Moreover, the synteny and evolutionary constraints analyses of the GRAS proteins among soybean and distinct species (two monocots and four dicots) provided more detailed evidence forGmGRASgene evolution.Cis-element analyses indicated that theGmGRASgenes may be responsive to diverse environmental stresses and regulate distinct biological processes. Besides, the expression patterns of theGmGRASgenes were varied in various tissues, during saline and dehydration stresses and during seed germination processes.

Conclusions

We conducted a systematic investigation of theGRASgenes in soybean, which may be valuable in paving the way for futureGmGRASgene studies and soybean breeding.
Efficacy of different dose of dexmedetomidine combined with remifentanil in colonoscopy: a randomized controlled trial
Authors: Jia Li , Xie Meng , Zhang Jing , Guo Jingyu , Tong Tong , Xing Yuying
Published: September 2020

Background

Dexmedetomidine has advantages during colonoscopy as it allows the patient to cooperate during the procedure. Few studies examined the dexmedetomidine-remifentanil combination. This study was to evaluate the effects of different doses of the dexmedetomidine-remifentanil combination in colonoscopy.

Methods

This was a prospective trial carried out at the Fourth Hospital of Hebei Medical University between 02/2018 and 10/2018. The patients were randomized: group I (dexmedetomidine 0.2 μg·kg− 1), group II (dexmedetomidine 0.3 μg·kg− 1), and group III (dexmedetomidine 0.4 μg·kg− 1), all combined with remifentanil. The primary outcomes were the patient’s body movements during the procedure and adverse events.

Results

Compared with at admission (T0), the SBP, HR, and RR at immediately after giving DEX (T1), at the beginning of the examination (T2), 5 min after the beginning of the examination (T3), 10 min after the beginning of the examination (T4), and at the end of the examination (T5) in the three groups were all reduced (all P < 0.05), but all were within the clinically normal range. SpO2 remained > 98% in all patients during the examination. Compared with T0, the BIS values of the three groups were decreased at T1 and T2 (all P < 0.05). There were no significant differences in BIS among the three groups (all P > 0.05). The minimum BIS value in group III was lower than in groups I and II (P < 0.05). The degree of satisfaction with the anesthesia effect was higher in groups II and III that in group I (P < 0.05). No hypotension occurred, seven patients had bradycardia, and four patients had nausea/vomiting.

Conclusions

Dexmedetomidine 0.3 μg·kg− 1 combined with remifentanil was effective for colonoscopy and had few adverse reactions.

Chinese Clinical Trial Registry: ChiCTR2000029105, Registered 13 January 2020 - Retrospectively registered.
Unveiling what is absent within: illustrating anesthetic considerations in a patient with hydranencephaly – a case report
Authors: Tovar Alexis R. , Thoeny Allison L.
Published: September 2020

Background

Hydranencephaly is a rare and debilitating congenital condition in which most anesthesiologists are unfamiliar. Primary surgical treatment involves CSF diversion, though other palliative procedures requiring anesthesia are often required. With medical advancements and a resulting prolonged life expectancy, caring for these patients is becoming more routine.

Case presentation

We follow an infant with hydranencephaly over three different procedures requiring anesthesia from 5 months of age to 2 years, highlighting the various anesthetic considerations.

Conclusions

Anticipation of difficult positioning, deliberate airway management, and attention to anesthetic recovery were all necessary to safely care for this patient. An understanding of the challenges this particular condition poses will help anesthesiologists provide the most safe and effective care when encountering these patients.
Effects of ipsilateral tilt position on the cross-sectional area of the subclavian vein and the clinical performance of subclavian vein catheterization: a prospective randomized trial
Authors: Yoon Hyun-Kyu , Lee Hyung-Chul , Kang Pyoyoon , Lee Jung-Man , Park Hee-Pyoung , Cho Youn Joung
Published: September 2020

Background

The cross-sectional area of the subclavian vein (csSCV) is a crucial factor in the successful catheterization of the subclavian vein. This randomized controlled study investigated the effects of the csSCV on landmark-based subclavian vein catheterization.

Methods

This study was performed using a two-stage protocol. During stage I, the csSCV was measured in 17 patients placed in the supine, 20° ipsilateral tilt, and 20° contralateral tilt positions in a random order. During stage II, landmark-based subclavian vein catheterization was randomly performed in patients placed in either the supine (group S, n = 107) or the ipsilateral tilt (group I, n = 109) position. The primary outcome measure was the csSCV in stage I and the primary venipuncture success rate in stage II. Secondary outcome measures were the time to successful venipuncture, the total catheterization time, the first-pass success rate, and the incidence of mechanical complications during catheterization.

Results

The csSCV was significantly larger in the ipsilateral tilt than in either the supine or contralateral tilt position (1.01 ± 0.35 vs. 0.84 ± 0.32 and 0.51 ± 0.26 cm2, P = .006 and < .001, respectively). The primary venipuncture success rate did not differ significantly between the group S and I (57.0 vs. 64.2%, P = .344). There were also no significant differences in the secondary outcome measures of the two groups.

Conclusions

The csSCV was significantly larger in patients placed in the ipsilateral tilt than in the supine position, but the difference did not result in better clinical performance of landmark-based subclavian vein catheterization.

Trial registration

NCT03296735 for stage I (ClinicalTrials.gov, September 28, 2017) and NCT03303274 for stage II (ClinicalTrials.gov, October 6, 2017).