bluuebunny/crossref_metadata_2025 huggingface dataset:
A scalable corneal xenograft platform: simultaneous opportunities for tissue engineering and circular economic sustainability by repurposing slaughterhouse waste
Authors: Wang Xinyu , Shakeel Adeeba , Salih Ahmed E. , Vurivi Hema , Daoud Sayel , Desidery Luca , Khan Raheema L. , Shibru Meklit G. , Ali Zehara M. , Butt Haider , Chan Vincent , Corridon Peter R.
Published: January 2023

High-throughput platforms that can generate copious quantities of corneal grafts will be invaluable in addressing the existing global demand for keratoplasty. Slaughterhouses generate substantial underutilized biological waste that can be repurposed to reduce current environmentally unfriendly practices. Such efforts to support ecological sustainability can simultaneously drive the development of transplantable ovine corneas and patient-specific bioartificial keratoprostheses. This study presents a dataset highlighting techniques that pioneered the development of a simplified, inexpensive, and high-throughput corneal xenograft platform. To the best of our knowledge, this is the first dataset to be generated in the United Arab Emirates devised on repurposing slaughterhouse waste in a manner that supports circular economic practices. These efforts outline the first step in developing a platform that can produce dozens of native ovine xenografts and acellular corneal scaffolds that effectively preserve ocular transparency, transmittance, and ECM components, and can be used to potentially create individualized and transplantable grafts. Such native grafts can then be genetically modified to reduce rejection and retrovirus transmission in recipients. Analogously, decellularization technologies can support corneal regeneration with key attributes comparable to native xenografts.
Light Spectra Have Minimal Effects on Rooting and Vegetative Growth Responses of Clonal Cannabis Cuttings
Authors: Moher Melissa , Llewellyn David , Golem Scott , Foley Elizabeth , Dinka Steve , Jones Max , Zheng Youbin
Published: January 2023

Until recently, most clonal cannabis (Cannabis sativa) has been propagated using fluorescent lights. Transitioning to light-emitting diodes (LEDs) may be a viable alternative to fluorescent lighting, enabling cultivators to provide specific spectrum treatments to enhance rooting while also saving energy. Vegetative stem cuttings of ‘Gelato-27’, ‘Grace’, and ‘Meridian’ were rooted for 15 days under various combinations of blue (B), red (R), ultraviolet-A (UVA) LEDs, phosphor-converted white (W) LEDs, and a fluorescent (F) control treatment, each with a canopy-level photosynthetic photon flux density (PPFD) of 200 µmol·m−2·s−1 and 16-hour photoperiod. The photon flux ratios of blue (B; 400–500 nm) and red (R; 600–700 nm) narrowband LED treatment combinations were (1) BR, fixed spectrum of B15:R85; (2) B, B75:R25 on day 0–2 followed by B15:R85 on day 2–14; (3) B+UVA, B75:R25 on day 0–2 followed by B15:R85 on day 2–14 plus 15 µmol·m−2·s−1 of UVA on day 7–14; (4) B50, B15:R85 on day 0–7 followed by B50:R50 on day 7–14. The W and F treatments both had static spectra. After the propagation period (i.e., plug stage), a portion of the cuttings under each treatment × cultivar combination were destructively harvested and the remainder were transplanted and grown vegetatively for an additional 21 days (i.e., transplant stage) under a PPFD of ≈275 µmol·m−2·s−1 from ceramic metal halide fixtures and then destructively harvested. Although there were no spectrum treatment effects on the percentage of cuttings that rooted, root index values were higher in cuttings grown under B+UVA vs. F. Further, relative root dry weights of plugs from the B, B+UVA, B50, and F treatments were higher than the W treatment. At the end of the plug stage, there were no spectrum treatment effects on the chlorophyll content index, cuttings grown under the B treatment had thicker stems compared with BR and W treatments, and cuttings grown under the F treatment exhibited the lowest percentage of new aboveground growth. None of the aforementioned spectrum treatment effects from the propagation stage persisted post-transplant. The use of LEDs is a promising, energy-efficient alternative to fluorescent lighting for cannabis propagation and B-enhanced spectrum treatments appear to enhance the rooting performance of clonal cannabis cuttings.
Strip-tillage and Zonal Cover Cropping Effects on Organic Squash Production
Authors: Brainard Daniel C. , Hayden Zachary D. , Benzle Marisa M. , Metiva Michael , Appenfeller Logan R. , Szendrei Zsofia
Published: January 2023

Zonal management of cereal–legume cover crop mixtures may help address weed and nitrogen management challenges common in organic reduced tillage systems. During a field study conducted over 3 years in Michigan, we evaluated the effects of cover crop management, tillage, and supplemental mulch on organically produced acorn squash (Cucurbita pepo). During the fall season before squash production, rye (Secale cereale L.) and vetch (Vicia villosa Roth) cover crop mixtures were sown in two distinct spatial arrangements: a “mixed planting,” in which seeds were sown in the same rows, and a “zonal planting,” in which vetch was planted only in the in-row zone and rye was planted only in the between-row zone of the subsequent squash crop. During the following spring season, cover crops were mowed, and four tillage and cover crop management combinations were established: full-width tillage with the mixed planting of rye–vetch (full-till mixed); strip-till with the same mixed planting (strip-till mixed); strip-till with the rye–vetch zonal planting (strip-till zonal); and strip-till with the zonal planting and additional rye mulch added between crop rows immediately after crop establishment (strip-till zonal plus rye). The strip-till mixed treatment resulted in yields equivalent to those of the full-till mixed treatment despite lower available nitrogen and greater early weed competition in some cases. Within strip-till treatments, zonal planting of rye–vetch provided no benefits relative to full-width planting (treatment 2 vs 3) and resulted in lower total cover crop biomass, a higher density of escaped weeds, and lower squash yields during 1 of 3 years. Supplemental rye mulch improved weed suppression and yields in strip-till zonal treatments and resulted in yields equivalent to those of the full-till mixed treatment in all years, but it provided no benefits relative to strip-till mixed. Our results demonstrate that strip-till organic squash production can produce yields equivalent to full-till production in Northern climates, but that zonal planting and supplemental mulch have limited benefits for addressing ongoing weed and nitrogen management challenges. Growers must weigh costs associated with these challenges against potential benefits for soil and pest regulating ecosystem services before adopting these agricultural conservation practices.
Optimizing Nitrogen, Phosphorus, and Potassium Fertilization Levels for Container Plants of Lagerstroemia Indica ‘Whit III’ Based on the Comprehensive Quality Evaluation
Authors: Wu Yijing , Lu Qingyu , Gong Yao , Zhang Yiming , Xu Yan , Cai Ming , Pan Huitang , Zhang Qixiang
Published: January 2023

To improve plant quality and fertilizing efficiency, we conducted a study to elucidate the effects of nitrogen (N), phosphorous (P), and potassium (K) fertilizers on the growth, nutrient accumulation, and quality of Lagerstroemia indica plants grown in containers and determine the optimal fertilization levels. Both single-factor and multifactor experiments involving N, P, K fertilizers were designed. Integrated with the plant growth, physiological traits, nutrient levels, and other indices, we used a membership function analysis to comprehensively evaluate plant quality. During the single-factor experiments, the best levels of the single fertilizers applied were 8 g/plant N, 2 g/plant P, and 4 g/plant K. We also found that, within a certain range, N, P, and K fertilizers promoted vegetative growth, increased the chlorophyll, soluble sugar, and soluble protein concentrations, and enhanced nutrient accumulation of L. indica. To avoid the wasting of fertilizers and promote plant quality, the optimal application levels were calculated using a regression analysis. The suggested N, P, and K applications were 6.89 g/plant, 1.97 g/plant, and 3.33 g/plant, respectively. Our results revealed that N, P, and K effect the performance of L. indica container plants, which paves the way for developing reliable and precise fertilizing techniques for growing L. indica.
Classroom Trade Show: An Alternative to Traditional Classroom Presentations in an Undergraduate Plant Identification Course
Authors: Miller Chad T.
Published: January 2023

Students enrolled in a landscape plant identification course were assigned a plant project at the beginning of the semester that comprised a written portion and a presentation portion. During the Spring 2014 semester, an electronic slide presentation portion was replaced by a “trade show” display presentation format. The modification was implemented to provide a more meaningful and applicable learning experience because trade shows are important events in the horticulture industry. Using this format, students were required to present or sell their plant genus by constructing an informative display, similar to that used for a tradeshow. Groups of students presented on different days. Students who were not presenting or selling were the “customers,” and they perused the trade show to learn about the various genera. A postactivity assessment survey was administered to gain insight into the student learning experience. The activity was evaluated during five spring semesters. Students responded that they were more comfortable with the trade show style of presentation than with a traditional presentation style, with a Likert scale rating of 1.5 (1–5). When asked to rate their ability to be creative with this activity, students agreed (Likert scale rating of 4.2) that the trade show activity allowed them to be creative. Eighty-nine percent of the students liked the activity. When asked to rate the activity compared with a more traditional speech in front of the class, students gave it a rating of 1.4, which is between excellent and above average. During the five semesters, nearly all students (97%) recommended that the trade show activity should be implemented again in future classes. The trade show format was well-received by students and can assist with the professional skills development of students.
Pedigree Analysis of Modern Muscadine Cultivars Reveals a Narrow Genetic Base
Authors: Conner Patrick J. , Bhattarai Gaurab , Williams Haley N. , Stafne Eric T.
Published: January 2023

Muscadine grape (Vitis rotundifolia var. rotundifolia) is a rare crop in that it has transitioned from a wild fruiting plant to a domesticated fruit within the past 150 years. Furthermore, this domestication process was carried out by just a few institutions that published copious records of the origin and traits of the first wild selections, goals and methods of the breeding programs, and the pedigrees of releases. We thus have a near complete record of the domestication of this interesting fruit crop. Early breeding efforts made use of fewer than a dozen wild selections, most of which were collected from the coastal plain of North Carolina and South Carolina. This narrow germplasm base has led to increasing levels of inbreeding in the most recent muscadine cultivar releases. To better understand the germplasm base of muscadine, the pedigrees of 54 muscadine cultivars released since 1970 were examined. Only 15 founders (founding clones) were identified that appeared in more than two cultivars, and five of these represent open pollination events that may not indicate the addition of new genetic material. By far the most used founder was ‘Scuppernong’, which appeared in 53 of 54 pedigrees and had an average genetic contribution of 22.8%. The remaining founders varied from 0.9% to 14.8% in their average genetic contribution. Coancestry coefficients between cultivars averaged 0.18, but were often much higher among recent fresh-market releases. Analysis of vine vigor as measured by trunk caliper in seedling progenies suggests that coancestry coefficients greater than 0.23 result in below average seedling vigor. The University of Georgia muscadine breeding program is evaluating multiple wild muscadine accessions to reduce inbreeding and increase the genetic diversity of its germplasm.
Inhibitive	Properties of Anredera Cordifolia Extract on Escherichia Coli Swimming Motility
Authors: Kristanto Alexander Tobias , Merry Maria Silvia , Hutomo Suryani
Published: January 2023

Background Escherichia coli, a Gram-negative bacterium, is one of the main etiology of urinary tract infection (UTI). An important pathogenicity factor influencing biofilm production is its motility, swimming, or swarming. Anredera cordifolia (AC) leaves have been proven to have an antibacterial effect on various Gram-positive and Gram-negative bacteria, including E. coli.Objective To determine the effect of AC leaves extract on swimming motility property of E. coli derived from UTI patients. Methods AC leaves were extracted with the maceration method. E. coli was identified and isolated from UTI patients. There are4 diluting concentrations of AC leaves extract (from 875 mg per ml to 7000 mg per ml) and one negative control. The preparation underwent incubation, inoculation, and re-incubation. Swimming motility was measured manually, and the result was tested for significant difference between each dose with one-way ANOVA (limit of significance is 0.05)Results On AC leaves extract concentration of 1750 mg per ml, the swimming motility has been inhibited significantly compared to negative control and lower concentration of 875 mg per ml (0.543 cm vs 1.673 cm and 1.358 cm, respectively; p < 0.001). Increasing concentration (3500 mg per ml and 7000 mg per ml) did not show a significant difference in inhibition compared to 1750 mg per ml (0.539 cm and 0.530 vs 0.543).Conclusion AC leaves extract with a concentration of at least 1750 mg per ml would inhibit the swimming motility of E. Coli derived from UTI patients.
BIOFILMS AND ANTIBIOTIC RESISTANCE
Authors: Jayadi Tejo
Published: January 2023

The physician and health practitionerâ€™s attention and alertness to biofilm existence and the consequences of biofilm in the biotic and abiotic environment are important to improve hygiene practice, rational antibiotic prescription, controlling the spread and emergence of emergency antibiotic resistance in healthcare facilities and community. The majority of chronic persistence and recurrent infection because of antibiotic resistance is biofilm infection, so needed precision and power alternative approach of management and therapy.
The Effect of Body Mass Index on Five Years Progression Free Survival in Breast Cancer
Authors: Probowati Wiwiek , Jayadi Tejo , Rustam Brenda miriane , Putri Agatha maheswari adita
Published: January 2023

Backgroud Breast cancer is the type of cancer that has the highest prevalence. Breast cancer growth is related to nutritional status. Nutritional status is assessed based on measurements of BMI Body Mass Index (BMI) and obesity is a factor that plays a role in the growth of cancer cells.Objective To investigate the effect of BMI on the five-year progression-free survival (PFS) of breast cancer patientsMethods A retrospective cohort study using observational methods on 82 female patients with breast cancer at Bethesda Hospital, Yogyakarta.Results A total of 82 medical records of breast cancer patients were collected at Bethesda Hospital Yogyakarta. A total of 71 patients (86.6%) were still alive, while 11 have died. This study found an average progression-free survival of BMI category <25 kg/m2 78,5 months while the BMI â‰¥ 25.0 kg/mÂ² 80,9 months (HR 2,057, 95% CI: .457â€“2.491). In addition, BMI and other prognostic variables did not have a significant relationship with progression-free survival in breast cancer patients (p = 0.114).Conclusion In this study, Body Mass Index was not associated with the 5-year progression-free survival of breast cancer patients.Â
Presentación del homenaje a D. Rafael Alvira Domínguez
Authors: Hurtado Domínguez Rafael
Published: January 2023

Presentación editorial
Sociedad civil y bien común
Authors: Mercado Ramírez Lourdes
Published: January 2023

El presente ensayo abordará los elementos que constituyen una sociedad civil, a partir de una exhaustiva exploración bibliográfica basada en el pensamiento del filósofo español Rafael Alvira, en la que se promueva el bien común, la verdad y la virtud, elementos esenciales para la mejora de cada uno de sus integrantes en lo personal y en su conjunto. En efecto, el ser humano aspira por su condición, como es lógico, a una sociedad humanizada, es decir, civilizada, civil, en la que la personalidad individual pueda desplegar todo su potencial, precisamente en la integración dentro de una sociedad armoniosa.
Nitrogen removal from wastewater by an immobilized consortium of microalgae–bacteria in hybrid hydrogels
Authors: Morán-Valencia Marien , Nishi Kento , Akizuki Shinichi , Ida Junichi , Cuevas-Rodríguez Germán , Cervantes-Avilés Pabel
Published: January 2023

The high content of nitrogen in wastewater brings some operational, technical, and economical issues in conventional technologies. The aim of this study was to evaluate the nitrogen removal by hybrid hydrogels containing consortium microalgae–nitrifying bacteria in the presence of activated carbon (AC) used as an adsorbent of inhibitory substances. Hybrid hydrogels were synthesized from polyvinyl alcohol (PVA), sodium alginate (SA), biomass (microalgae–nitrifying bacteria), and AC. The hybrid hydrogels were evaluated based on the change in ammonium (NH4), nitrate (NO3), and chemical demand of oxygen (COD) concentrations, nitrification rate, and other parameters during 72 h. Results indicated that NH4 removal was more effective for hydrogels without AC than with AC, without significant differences regarding consortium biomass concentration (5 or 16%), presenting final concentrations of 3.13 and 3.75 mg NH4/L for hydrogels with 5 and 16% of the biomass, respectively. Regarding NO3 production, hydrogels without AC reached concentrations of 25.9 and 39.77 mg NO3/L for 5 and 16% of the biomass, respectively, while treatments with AC ended with 2.17 and 1.37 mg NO3/L. This confirms that hydrogels can carry out the nitrification process and do not need AC to remove potential inhibitors. The best performance was observed for the hydrogel with 5% of biomass without AC with a nitrification rate of 0.43 mg N/g TSS·h.
The development of "socialism with chinese specifics in a new era": to the results of the XX congress of the communist party of China
Authors: Avdokushin E. F.
Published: January 2023

The article the author notes that China approached the twentieth Congress of the CPC with contradictory results. On the one hand, there are many important, profound, historical achievements that have transformed the People's Republic of China in various fields, strengthening its position as the second economy of the world. And on the other hand, there are a number of problems left over from the previous period of reform and development, as well as new challenges to the world economy, politics, and the coronavirus pandemic. The article analyzes China's achievements in socio-economic life, reveals the problems and tools to solve them. In the article, the author puts forward the thesis about the division of China's economic reform into two major, fundamental periods – the period of adaptation, localization of foreign innovations and technologies and the period of transition to development based on their own, national innovations. The author reveals the main features of the second period in the development of the Chinese economic reform, including the course towards a new development architecture, emphasizes both the presence of achievements and problems. It is announced that after the twentieth Congress of the CPC, a new campaign will begin in the country on the way to the realization of the Chinese dream with great hopes and determination.
Special operation "Cancellation of Russia" on the world market: first results
Authors: Maltsev A. A.
Published: January 2023

The article examines the first most important consequences of the Western bloc's sanctions policy for the global and Russian economy. The purpose of the work: on the basis of systematization of sanctions instruments grouped into 3 packages – financial blockade, foreign economic barriers, curtailment of scientific and technical cooperation, to check the mutual effectiveness of their aftereffects. As a result of the author's analysis of the practice of sanctions pressure on the example of the US economy, the European Union and the Russian Federation, the working hypothesis of the study was confirmed: the complete isolation of the Russian economy sought by the initiators of sanctions pressure did not take place. However, the sanctions policy of the West has had a significant impact on the system of the world economy. And this influence is multidirectional and generally negative. Long-term economic growth in conditions of relative external isolation will now have to be ensured by the domestic economy of the Russian Federation, relying on the preservation and multiplication of human capital and with the help of broad state protectionism. The author suggests possible directions of adjustment of the Russian general economic policy: the docking of a set of measures for the near, medium and long-term prospects; priority development of the domestic economy with an emphasis on the preservation and augmentation of human capital; exclusion of options for autarkic development.
Russian-Hungarian cooperation in the pharmaceutical sector and the impact of anti-russian sanctions
Authors: Bakó Tamás , Aidrоus Irina Ahmed Zein
Published: January 2023

The analysis of the possibilities and directions of Russian-Hungarian bilateral cooperation in the field of pharmaceuticals carried out by the authors allowed us to assert that in the conditions of anti-Russian Western sanctions, the situation in the domestic pharmaceutical industry in terms of demand and supply of medicines has significantly worsened. The pharmaceutical industry remains important both from the point of view of ensuring public health and national security in this area, and high innovation. The authors conclude that it is necessary to strengthen international cooperation in this area, especially with states that traditionally have high achievements in it and a long history of interaction with Russian structures. One of these countries is Hungary. The article emphasizes that both countries are interested in cooperation in this area, relying on the ties traditionally formed during the socialist period and a certain complementarity. It is also noted that under the conditions of sanctions, the Russian pharmaceutical industry maintains both positive dynamics and maintains a high level of competitiveness of manufacturers in the domestic market.
Control de Botrytis cinerea Pers. en tomate cv. “Huascarán” mediante fertilizantes foliares en la costa de Lima, Perú
Authors: Casimiro Melgarejo Hugo Benny , Aragón Caballero Liliana María , Casas Díaz Andrés Virgilio , Bustamante Huaman Norma
Published: January 2023

Botrytis cinérea Pers. (B.C.) es el fitopatógeno responsable de una amplia gama de síntomas en cultivos de gran importancia como el tomate. Su control principalmente se basa en la aplicación de fungicidas químicos; sin embargo, su uso excesivo conduce al desarrollo de cepas resistentes, contaminación ambiental y efectos perjudiciales para la salud humana. Por ello, el objetivo el estudio fue evaluar el efecto de dos fertilizantes foliares que contienen cobre, zinc y ácido cítrico en el control del moho gris B.C. en tomate cv. Huascarán. En condiciones de laboratorio se evaluó el efecto de los fertilizantes foliares en la inhibición del crecimiento micelial a los 6 DDI (días después de inoculación) con el método del medio envenenado en PDA (agar de papa y dextrosa); y en condiciones de campo se aplicaron los fertilizantes foliares por ocho semanas variando la frecuencia de aplicación en una y dos semanas. Los resultados mostraron que en condiciones de laboratorio el ácido cítrico incrementó la actividad antifúngica complementándose bien con las partículas de Cu y Zn presentando el T3 el mejor porcentaje de inhibición del crecimiento micelial. En condiciones de campo la mayoría de las aplicaciones tuvieron un control significativo de B.C. en el follaje, pero no en flores ni en frutos; destacando aquellos donde se aplicó los fertilizantes foliares semanalmente. Estos resultados son importantes para desarrollar estrategias dirigidas a mejorar el control de la enfermedad y disminuir el uso masivo de fungicidas.
USO DE LA PALOMA DE CASTILLA (Columba livia) COMO BIOMONITOR DE CONTAMINACIÓN POR METALES TRAZA EN LIMA, PERÚ
Authors: Guevara-Torres Diego R. , Williams Marta , Palacios Gloria
Published: January 2023

Los metales traza continúan generando problemas de contaminación ambiental debido a su persistencia en el ambiente, representando un peligro para los seres vivos. Estudios recientes han reportado contaminación de metales pesados en suelos de algunas partes de Lima, sin embargo, queda aún mucho por explorar. El uso de la paloma de Castilla (Columba livia) como biomonitor podría facilitar evaluaciones rápidas y de detección de contaminación por metales traza en el ambiente. En este estudio, se determinó las concentraciones de plomo (Pb), cadmio (Cd), zinc (Zn), cobre (Cu), molibdeno (Mo), selenio (Se), hierro (Fe) y estroncio (Sr) en el hígado de 21 palomas de tres localidades con características de uso industrial, urbano y rural.  Nuestros resultados mostraron que las concentraciones de Pb, Zn, Cu, Se, Fe y Sr eran más elevadas en las localidades industrial y urbana que en la rural. Esto sugiere la existencia de una gradiente con concentraciones de metales traza más elevadas en las localidades industrial y urbana. Dicho gradiente es consistente con las características del uso de la tierra de cada localidad y corrobora los problemas de contaminación ambiental asociados a ellas. Nuestro estudio expone el potencial de la paloma de Castilla para actuar como biomonitor de la contaminación por metales traza en Lima y otras ciudades del Perú y del mundo. Según nuestro conocimiento, esta es la primera evaluación de metales traza hecha con palomas en Perú.
GPCR Allostery: A View from Computational Biology
Authors: Li Mengrong , Bao Yiqiong , Li Miaomiao , Guo Jingjing
Published: January 2023

G protein-coupled receptors (GPCRs) represent a large superfamily of cell-surface
proteins that mediate cell signaling and regulate virtually various aspects of physiological
and pathological processes, therefore serving as a rich source of drug targets. As
intrinsically allosteric proteins, numerous functions of GPCRs are regulated via allostery,
whereby allosteric modulators binding at a distal site regulate the function of the typical
orthosteric site. However, only a few GPCR allosteric ligands have been presently approved
as drugs due to the high dynamic structures of GPCRs. Fortunately, the rapid development
of computational biology sheds light on understanding the mechanism of
GPCR allosteric ligands, which is critical for the discovery of new therapeutic agents.
Here, we present a comprehensive overview of the currently available resources and approaches
in computational biology related to G protein-coupled receptor allostery and
their conformational dynamics. In addition, current limitations and major challenges in
the field are also discussed accordingly.
Exploration and Practice of Medicine Pathway for Perioperative VTE Prophylactic
Anticoagulant Treatment
Authors: Li Jing , Zhang Bin , Zhang Chuanzhou , Li Yongxin , Zhou Changkai , Shi Shiqiang , Ji Hongyan
Published: January 2023

Objective:

Using perioperative VTE prophylactic anticoagulant pathway delicacy management, we
aimed to explore the role of clinical pharmacists in perioperative VTE prophylactic anticoagulant treatment and
the effect of the clinical medicine pathway implementation.

Methods:

In compliance with evidence-based medicine, combined with clinical practice, clinical pharmacists
participated in the formulation of the perioperative anticoagulant clinical medicine pathway. The PDCA cycle
was strictly implemented. Urologic Department, Affiliated Hospital of Qingdao University, Qingdao Shandong,
China, served as a pilot department for the evaluation of the effect of drug clinical pathway implementation.

Results:

The anticoagulant clinical medicine pathway for the urologic department was formulated and implemented
from October 2020 to August 2021. The entry rate gradually increased, and the accuracy of thrombosis
risk assessment improved. The proportion of drugs in the department and the per capita drug cost decreased significantly.

Conclusion:

The perioperative VTE prophylactic anticoagulant pathway showed significant achievements in
the rational use of urologic anticoagulant drugs, lowering the cost of medical care. Therefore, it could be considered
a novel long-term mechanism for rational analgesia drug use.
Therapeutic Potential of Herbal Medicine against Non-alcoholic Fatty
Liver Disease
Authors: Avan Amir , Pourali Ghazaleh , Hosseini Zeinab S. , Maftooh Mina , Nazari Elham , Khazaei Majid , Nassiri Mohammadreza , Hassanian Seyed M. , Ghayour-Mobarhan Majid , Ferns Gordon A. , Kiani Mohammad A.
Published: January 2023

Non-alcoholic fatty liver disease (NAFLD) is a common metabolic disorder associated
with obesity, diabetes mellitus, dyslipidemia, and cardiovascular disease. A “multiple hit” model
has been a widely accepted explanation for the disease's complicated pathogenesis. Despite advances
in our knowledge of the processes underlying NAFLD, no conventional pharmaceutical
therapy exists. The only currently approved option is to make lifestyle modifications, such as dietary
and physical activity changes. The use of medicinal plants in the treatment of NAFLD has recently
gained interest. Thus, we review the current knowledge about these agents based on clinical
and preclinical studies. Moreover, the association between NAFLD and colorectal cancer (CRC),
one of the most common and lethal malignancies, has recently emerged as a new study area. We
overview the shared dysregulated pathways and the potential therapeutic effect of herbal medicines
for CRC prevention in patients with NAFLD.
Feasibility of Imaging-Based Quantitative Hepatic Function Assessment:
Gd-EOB-DTPA-Enhanced Liver MR Imaging Combined with T1 Mapping
Authors: Dong Zhi , Wang Meng , Feng Yanqing , Cai Huasong , Feng Shi-Ting , Li Zi-Ping , Lin Zhi , Peng Zhenpeng
Published: January 2023

Objective:

to investigate the feasibility of gadoxetic acid (Gd-EOB-DTPA) enhanced MRI
combined with T1 mapping in quantitative hepatic function assessment.

Methods:

this study retrospectively enrolled 94 patients with Gd-EOB-DTPA enhanced MRI combined
with T1 mapping, divided into group A (grade A, n=73), group B (grade B, n=14) and group C
(grade C, n=7) based on Child-Pugh classification. Liver T1 relaxation times on plain scan (T1P) and
hepatocellular phase (T1E) were measured. Decrease in T1 (T1D) and the percentage of decrease in T1
(T1D%) were calculated as follows: T1D=T1P-T1E, T1D%= T1D/T1P×100%. The relationship between
T1P, T1E, T1D, T1D% and liver function classification was analyzed.

Results:

T1P, T1D, and T1D% in group A were significantly higher than those of group B and C. T1E in
group A was lower than those of group B and C. T1D% was significantly different between group B
and C. There was no significant difference in T1P, T1E, T1D between groups B and C. T1E was positively
correlated with liver function levels, T1P and T1D had a negative correlation with liver function levels.
T1P, T1E, T1D, T1D% were significantly different between cirrhotic and non-cirrhotic groups.
T1D% of less than 70% suggests liver dysfunction.

Conclusion:

Gd-EOB-DTPA enhanced liver MRI combined with T1 mapping is feasible for quantitative
assessment of hepatic function.
Primary Epiploic Appendagitis: Not Rare But Rarely Diagnosed Pathology
Authors: Kahveci Servet , Zitouni Soubhi , Abubakar Abdul Razak , İkizceli Turkan , Ozturk Mehmet , Aktürk Adem , Aksu Yusuf
Published: January 2023

Objective:

The aim of this study is to determine the clinical and laboratory parameters
which may be suggestive of or even pathognomonic for primary epiploic appendagitis (PEA) and to
discuss the diagnostic efficacy of ultrasound (US) compared to computed tomography (CT) in patients
with PEA.

Materials and Methods:

For this retrospective study, 92 patients diagnosed with PEA using US, CT or
both modalities were included. All patient symptoms, clinical findings and laboratory parameters were
reviewed. The CT and US images of the PEA were evaluated for lesion size and location, the relationship
of the lesion to the colon and the distance of the lesion to the skin.

Results:

There were 16 female and 76 male patients in the study group. The mean age was 35 years
(range: 38–79 years). Well-localized abdominal pain was the primary symptom in all patients. The
mean leukocyte count was 7857±1326 mm-3. The most frequent localization of PEA was sigmoiddescending
colon junction (79/92). In patients who were examined by both US and CT, the size of the
fatty central core was between 15-48 mm (mean:28.10 mm) and 9-22 mm (mean:15.07 mm) in its
long-axis and short-axis diameter, respectively on US, whilst that by CT was between 15-46 mm
(mean:26.88 mm) and 9-21 mm (mean:14.40 mm) in its long-axis and short-axis diameter, respectively.
In patients who were examined by both US and CT, the mean distance of the lesions to the skin was
20.80 mm and 33.97 mm, respectively. All patients were treated conservatively with complete resolution
of symptoms within a week of presentation.

Conclusion:

PEA is an unrare self-limiting condition that should be considered in the differential diagnosis
of acute abdomen. To support clinicians and radiologists regarding PEA and its clinical, laboratory
and radiological findings, targeted sonographic examination - which is radiation and contrast
agent-free - could be highly sufficient for the diagnosis of PEA and may prevent unnecessary further
imaging and mistreatment.
Application of Microwave and Ultrasonication for Anthocyanin Extraction
Process from Garcinia indica Choisy Fruit Waste and Assessment of
Antioxidant Activity using Caenorhabditis elegans Model
Authors: Shastry Rajesh Padumane , Sriramareddy Srinath Boreddihalli
Published: January 2023

Garcinia indica (also known as kokum) is a small evergreen tree that has been used in a
variety of culinary, industrial, and pharmacological products, as well as fruit juices and food. In the
present study, the antioxidant capacity of anthocyanin extracted from Garcinia indica fruit waste
was assessed using DPPH, ABTS assay, and a Caenorhabditis elegans infection model. The
independent variables, such as temperature, solvent concentration, microwave exposure, and
exposure to ultrasonication were integrated as independent variables in a five-level central
composite design using response surface methodology. Based on statistical analysis, the generated
models were successfully utilised to analyse the experimental data and determine the best
extraction conditions. The rescue effect of anthocyanin was further studied using a paralysis and
killing assay in a C. elegans infection model. The extraction yield was 21.0 mg/g under these
conditions, with antioxidant activity of 9.9 μg/ml by ABTS assay and 6.6 μg/ml by DPPH assay,
respectively. Furthermore, as compared to ethanol leaching extraction, this experimental design
increased anthocyanin yield by more than 15 fold. The treatment of anthocyanin with C. elegans
from E. coli and Pseudomonas aeruginosa PAO1 infection resulted in a significantly longer
lifetime. Garcinia indica fruit waste extracts high in anthocyanins might be employed as natural
food colorants and antioxidant additives in food products.
Identification of Novel Peptides as Potential Modulators of Aβ42 Amyloidogenesis:
An in silico Approach
Authors: Kundal Kavita , Paramasivam Santhosh , Mitra Amit , Sarkar Nandini
Published: January 2023

Aims:

Alzheimer's disease is a neurodegenerative disease for which no cure is available.
The presence of amyloid plaques in the extracellular space of neural cells is the key feature of
this fatal disease.

Background:

The proteolysis of Amyloid Precursor Protein by presenilin leads to the formation of
Amyloid-beta peptides (Aβ 42/40). Deposition of 42 residual Aβ peptides forms fibril’s structure,
disrupting neuron synaptic transmission, inducing neural cell toxicity, and ultimately leading to
neuron death.

Objective:

Various novel peptides have been investigated via molecular docking and molecular
dynamic simulation studies to investigate their effects on Aβ amyloidogenesis.

Methods:

The sequence-based peptides were rationally designed and investigated for their interaction
with Aβ42 monomer and fibril, and their influence on the structural stability of target proteins
was studied.

Results:

Analyzed docking results suggest that the peptide YRIGY (P6) has the highest binding
affinity with Aβ42 fibril amongst all the synthetic peptides, and the peptide DKAPFF (P12) similarly
shows a better binding with the Aβ42 monomer. Moreover, simulation results also suggest
that the higher the binding affinity, the better the inhibitory action.

Conclusion:

These findings indicate that both the rationally designed peptides can modulate amyloidogenesis,
but peptide (P6) has better potential for the disaggregation of the fibrils. In contrast,
peptide P12 stabilizes the native structure of the Aβ42 monomer more effectively and hence can
serve as a potential amyloid inhibitor. Thus, these peptides can be explored as therapeutic agents
against Alzheimer's disease. Experimental testing of these peptides for immunogenicity, stability
in cellular conditions, toxic effects and membrane permeability can be the future research scope of
this study.
Hypoxia and Hypoxia Mimetic Agents As Potential Priming Approaches to
Empower Mesenchymal Stem Cells
Authors: Yasan Goknur Topaloglu , Gunel-Ozcan Aysen
Published: January 2023

Mesenchymal stem cells (MSC) exhibit self-renewal capacity and multilineage differentiation
potential, making them attractive for research and clinical application. The properties of MSC can vary
depending on specific micro-environmental factors. MSC resides in specific niches with low oxygen concentrations,
where oxygen functions as a metabolic substrate and a signaling molecule. Conventional
physical incubators or chemically hypoxia mimetic agents are applied in cultures to mimic the original
low oxygen tension settings where MSC originated.

:

This review aims to focus on the current knowledge of the effects of various physical hypoxic conditions
and widely used hypoxia-mimetic agents-PHD inhibitors on mesenchymal stem cells at a cellular and
molecular level, including proliferation, stemness, differentiation, viability, apoptosis, senescence, migration,
immunomodulation behaviors, as well as epigenetic changes.
Isolinderalactone Resistance to the Liver Injury Induced by Oxaliplatin in Rats
Through Inhibiting IL-6/STAT3 Signal Pathway
Authors: Lin Rongwei , Zhou Yang , Hu Chao , Yao Hongqing , Jin Wangxun
Published: January 2023

Background:

Oxaliplatin (OXA) is easy to cause sinusoidal obstruction syndrome (SOS), leading to liver
injury. Isolinderalactone (ILL), one of the main components of Lindera aggregate, has been reported to have a protecting
effect on the liver. However, it is unclear whether ILL has a therapeutic effect on liver injury caused by OXA. This
study aims to determine the effect of ILL on the prevention and treatment of OXA-induced liver injury and to provide
a basis for the chemotherapy of gastrointestinal tumors.

Methods:

Intraperitoneal injection of folinic acid, 5-fluorouracil, and OXA was administered on the SOS rat model for
7 weeks. The indexes of liver function were measured by biochemical kit. The ratio of liver weight to body weight was
calculated. The pathological analysis of the liver was scored with the SOS scoring standard, fibrosis was evaluated
with a four-point scale. The expression of inflammation factors was detected by Real-Time PCR, and the related indexes
of IL-6/STAT3 were examined by Western blot analysis.

Results:

ILL down-regulated the portal vein pressure and alleviated the abnormal liver function of SOS rats and improved
the liver lesions. ILL inhibited the SOS by inhibiting IL-6/STAT3.

Conclusion:

ILL resistance to liver injury through inhibiting IL-6/STAT3 signal pathway.
Choking Susceptibility and the Big Five Personality Traits
Authors: Thiessen Burgandy , Sullivan Philip , Gammage Kimberley , Dithurbide Lori
Published: January 2023

Background:

Choking susceptibility is the likelihood or potential of an individual choking under pressure. Choking susceptibility can be influenced by personality traits.

Objective:

The purpose of this study is to examine the differences between the Big Five personality traits on choking susceptible and choking non-susceptible individuals from a Canadian University using a cross-sectional design. It was hypothesized that choking susceptibility could be predicted by the Big Five personality traits.

Methods:

A protocol developed by Mesagno and colleagues, comprising a self-consciousness scale, sports anxiety scale, and coping style scale, was used to measure choking susceptibility. The protocol has only been used within athlete populations. This study is the first to use the choking susceptibility protocol outside of sports, specifically for undergraduate students (N = 177).

Results:

A logistic regression revealed that the personality traits could significantly predict choking susceptibility. Neuroticism was the sole significant predictor. Higher neuroticism values significantly increased the probability of an individual choking susceptible.

Conclusion:

According to the current study, neuroticism predicted choking susceptibility. Future research should address choking susceptibility in different contexts and more closely examine the relationship between choking susceptibility and actually choking under pressure.
Perceived Stress and Association with Sociodemographic, Interpersonal Relationship and COVID-19 Lockdown Related Stress in South Africa
Authors: Maepa Mokoena , Kleinhans Atholl , Lentoor Antonio G.
Published: January 2023

Background:

COVID-19 pandemic was declared a global public health emergency in March 2020. South Africa, like many countries, was not spared from this pandemic. In March 2020, the president announced a nationwide lockdown with social restrictions aimed to curb the spread of the virus. Such lockdown restrictions disrupted the normal day to day life for South Africans, leading to personal stress.

Objective:

The current study aimed to explore perceived stress and lockdown related stress among South African adults during the first wave of COVID 19.

Methods:

This study was cross-sectional in nature, using a sample of 203 adult males and females who were selected using a convenience sampling method on online social media platforms such as WhatsApp and Facebook. Participants completed an online survey that assessed socio-demographic information, perceived stress and lockdown related stress.

Results and Discussion:

A significant statical difference between males and females regarding their experience of lockdown related stress t (281) = 1.35, p < .004 was found. Relationship status was also significant for lockdown related stress t (281) = -2.02, p< .001 and perceived stress t= (281) = -.08, p< .000. No significant statistical difference between young and older adults in their experience of lockdown related stress and perceived stress was found.

Conclusion:

Male participants reported more lockdown related stress, while female participants reported more perceived stress. There were no age differences in lockdown related stress and perceived stress. People without intimate relationships reported more lockdown related stress and perceived stress than people with intimate relationships.
Systematic Review and Meta-analysis of the Relationship Between Proton
Pump Inhibitors and Esophageal Adenocarcinoma
Authors: Darvishi Mohammad , Soureshjan-Heidari Saeid , Bidares Matin
Published: January 2023

Introduction and Aim:

Esophageal adenocarcinoma (EAC) mortality continues to increase
across the world. This meta-analysis was aimed to investigate the relationship between proton
pump inhibitors (PPIs) and the risk of EAC.

Methods:

This meta-analysis was done as per the PRISMA checklist using relevant keywords. To
this end, an extensive search was done on 29/6/2022 in EMBASE, Web of Science (ISI), PubMed,
and Scopus. In this study, 95% confidence interval (CI) and standardized mean difference (SMD)
were used to estimate the overall effect size. Analysis of the odds ratio (OR) for EAC was done
using a random effects model.

Results:

A total of 20 studies were included in the review. Compared to the group that received
PPIs, the OR of EAC in the recipients of the PPIs group was obtained at 0.67 (95% CI = 0.39-1.29,
P = 0.240). The meta-regression, including year, follow-up time, study design, sample size, quality
of the study, study period, and geographical location, demonstrated no source of heterogeneity (P
> 0.10).

Conclusion:

No significant relationship was found between PPIs use and the risk of EAC. Accordingly,
PPIs do not have a protective or risk factor effect on EAC.
UCDM: A User-Centric Data Model in Power Blockchain
Authors: Lv ZhiXing , Yu Hui , Kang Kai , Li Teng Chang , Du Guo Li
Published: January 2023

Background:

As innovative information technology, blockchain has combined the
advantages of decentralization, immutability, data provenance, and contract operation automatically,
which can be used to solve the issues of single point failure, high trading cost, low effectiveness,
and data potential risk in power trading. However, in the traditional power blockchain,
the design of functional components in blockchain, such as the data structure of the block, does
not take the actual features of power into account, thus leading to a performance bottleneck in
practical application. Motivated by business characteristics of power trading, a user-centric data
model UCDM in consortium blockchain is proposed to achieve efficient data storage and quick
data retrieval.

Methods:

The proposed UCDM is designed by considering the requirements of transaction retrieval
and analysis, thus supporting the requirements of concurrent data requests and mass data
storage. The ID of each user will independently form its own chain over the blockchain.

Results:

Compared with the traditional data model, the extensive experimental results demonstrate
that the proposed UCDM has shorter processing delay, higher throughput, and shorter response
latency, thus having practical value.

Conclusion:

UCDM is an effective solution to the transaction retrieval and analysis in power
blockchain. Furthermore, the participant of the blockchain network has a unique identity over the
world, which ensures high security during trading.