bluuebunny/crossref_metadata_2025 huggingface dataset:
पारिभाषित आँखा उपन्यासमा शरीरमाथि उपभोक्तावादी संस्कृतिको प्रभाव र प्रयोग
Authors: पौडेल शान्ति
Published: June 2023

प्रस्तुत शोधलेख पारिजातद्वारा लेखिएको पारिभाषित आँखाहरू उपन्यासमा चित्रित शरीर राजनीतिअन्तर्गत शरीरमाथि उपभोक्तावादी संस्कृतिको प्रभाव र प्रयोगको अध्ययनसँग सम्बद्घ छ । यसमा शक्तिशाली वर्गले शक्तिहीन वर्गको शरीरमाथि गर्ने शोषण तथा दमनका साथै शक्तिशाली वर्गले आफ्नो अनुकूलको विमर्शद्वारा केकसरी कमजोर वर्गको शरीरमाथि शासन गरी कमोडिटीका रूपमा रूपान्तरण गरेको छ भन्ने जिज्ञासाको समाधान खोज्ने प्रयत्न गरिएको छ । यसका लागि शरीरमाथि उपभोक्तावादी संस्कृतिको प्रभाव र प्रयोगसम्बन्धी मान्यतालाई कृतिविश्लेषणको मुख्य आधार बनाइएको छ । अध्ययनका अन्त्यमा शक्तिशाली वर्गले विभिन्न विमर्शमार्फत नारी शरीरको विनिर्माण गरी वस्तुमा रूपान्तरण गरेको सन्दर्भलाई सघन रूपमा उठाएको छ । शक्तिहीन वर्गले आफ्नो स्व जोगाउनका लागि गरेको प्रतिरोध असफल भएको सन्दर्भ यथार्थका साथ प्रस्तुत गरेको निष्कर्ष निकालिएको छ ।
دور مشاركة المعرفة في تعزيز السعادة الوظيفية – دراسة استطلاعية لآراء عينة من موظفي كلية العمارة الأهلية الجامعة
Authors: م.م ابراهيم صالح حسن  , م.م مصطفى كريم سهم  , م.م هديل قاسم عليوي
Published: June 2023

يسعى البحث الى تحليل علاقة الأرتباط والتأثير بين مشاركة المعرفة في مكان والسعادة الوظيفية . وتمثلت مشكلة البحث في تساؤل مهم وهو(هل هنالك علاقة ارتباط وتاثير بين مشاركة المعرفة في مكان العمل والسعادة الوظيفية) ولتوضيح العلاقة بين متغيرات البحث تمت صياغة فرضيتين رئيستين للأرتباط والتأثير . كما تم اعتماد الاستبانه كاداة رئيسية لجمع البيانات اذ شملت العينة (40) موظفاً من مجموع المجتمع الكلي البالغ (53) موظفاً في كلية العمارة الاهلية الجامعة, ولتحليل البيانات تم استخدام البرنامج الاحصائي SPSS وبرنامج EXCEL ومن الاساليب الاحصائية المستخدمة هي (التكرارات والاوساط الحسابية والانحراف المعياري ومعامل الارتباط سبيرمان ومعامل الانحدار الخطي البسيط) .
وقد توصل البحث الى نتائج عديدة اهمها هنالك مستوى عال من مشاركة المعرفة بأبعادها الفرعية في كلية العمارة الجامعة , فضلا عن وجود علاقة ارتباط معنوية وتأثير طردي بين مشاركة المعرفة والسعادة الوظفية
The Role of NMDA Receptors in General Anesthesia
Authors: Aronstam Robert S.
Published: June 2023

The NMDA receptor is a subtype of glutamate receptor that is widely-expressed in the central nervous system and mediates excitatory neurotransmission by opening an intrinsic ion channel in response to glutamate binding. We have demonstrated that the activation of NMDA receptors is inhibited by several volatile liquid general anesthetics. In the present study, tritiated probes for the glutamate recognition site and the ion channel, as well as direct measurements of calcium uptake, were utilized to determine the mechanism by which anesthetics interfere with NMDA receptor function. Multiple effects of volatile anesthetic were revealed: 1) at the glutamate recognition site, anesthetics inhibited [3H]CGS 19755 binding in a non-competitive manner; 2) glutamate-stimulation of [3H]MK- 801 binding to a site within the ion channel was markedly inhibited by anesthetics in a reversible, non-competitive, and glycine- sensitive manner; 3) anesthetics inhibited NMDA-stimulated 45Ca uptake by brain microvesicles in a glycine-sensitive manner; and 4) anesthetics decreased receptor densensitization. All of these in vitro effects of anesthetic inhibition were obtained within concentration ranges that are routinely achieved during surgical anesthesia. Anesthetic inhibition does not appear to involve direct actions with either the agonist recognition site or the receptor ion channel; the possibility that anesthetics act at obligatory allosteric sites (eg, the glycine site) to disrupt NMDA receptor-mediated neurotransmission requires further evaluation.
On the Limits of Islamic Cinema: The Politics of Halal Cinema Discourse in Kerala, India
Authors: Kunnummal Ashraf
Published: June 2023

Some of the most crucial debates surrounding Islamic legitimacy and cinema rotate around the question of the permissible ( ḥalāl) and prohibited ( ḥarām) in Islamic law and how these function for Muslim theology and politics. Emerging from these debates, halal cinema has been one of the recent critical interventions in the field of Indian and Islamic cinema. Rather than making cinema Islamic from an ideological position, the new halal experiments with the visual language of cinema without compromising the popular dimensions of its viewership. This article looks at the politics of halal cinema within the context of the state of Kerala in South India and argues that the space of halal cinema shows the aspirations and negotiations of the Muslim community in Kerala. Halal cinema thus becomes an immanent critique of Islamic cinema by destabilizing the dominant practice that connects the relationship between Islam and cinema.
Thirty years of research on high-growth entrepreneurship: bibliometric overview of its H-Classics
Authors: Lechuga-Sancho María-Paula , Martínez-Fierro Salustiano , Ramos-Rodríguez Antonio R.
Published: June 2023

In recent years, high-growth entrepreneurship (HGE) research has gained increasing importance. For this reason, it is considered necessary to analyze papers that have had the most significant impact on the development of the discipline and that should be familiar to all researchers. Building new knowledge on these works is important because it provides legitimacy and coherence to the future development of this research field. Thus, this paper aims to identify and characterize the classic articles in the field of HGE, a line of research that has seen significant growth in the last 30 years. The H-Classics method is used to identify these papers. Subsequently, several bibliometric aspects of this collection are analyzed, such as the forums of journals where they have been published, the most productive authors, the patterns of collaboration, and an analysis of the conceptual structure through co-word analysis. Exhaustive content analysis is carried out to complement this vision, identifying the proposed objectives, methodologies, types of data, analysis techniques used, and their main contributions in three consecutive periods. The results are of value to researchers interested in high-growth firms because they allow us to understand the foundations on which this discipline has been built through its classics and to determine its main challenges for the future.
Negacionismo bibliomÃ©trico
Authors: Torres-Salinas Daniel
Published: June 2023

Este texto discute y denuncia el creciente â€œnegacionismo bibliomÃ©tricoâ€, una tendencia que rechaza la utilidad de los indicadores bibliomÃ©tricos en la evaluaciÃ³n cientÃ­fica. Este negacionismo es evidente en la decisiÃ³n de prescindir de las mÃ©tricas, una prÃ¡ctica fomentada por la DeclaraciÃ³n Dora y, especialmente, con la firma del Agreement on Reforming Research Assessment promovido por Coara. En ambos casos, se aboga por un enfoque mÃ¡s cualitativo, basado principalmente en la revisiÃ³n por pares. Sin embargo, se argumenta que la adopciÃ³n de estos enfoques cualitativos se ha realizado sin considerar sus limitaciones e ignorando los principios y mÃ©todos de la BibliometrÃ­a evaluativa.
AnÃ¡lisis de datos y gestiÃ³n taxonÃ³mica en las actividades profesionales de la informaciÃ³n
Authors: Moreiro-GonzÃ¡lez JosÃ©-Antonio
Published: June 2023

Los anuncios de ofertas de trabajo para el sector LIS incluyen con efecto creciente a los profesionales de la informaciÃ³n en los equipos que analizan datos y los ordenan en Sistemas de OrganizaciÃ³n del Conocimiento (SOC). Se trata de procesar y visualizar datos de empresas y organizaciones a partir de la capacidad para preparar y analizar datos y encajar en equipos de investigaciÃ³n multidisciplinares. Para organizar los resultados del anÃ¡lisis de datos, productos y otros objetos de contenido emplean SOC, sobre todo taxonomÃ­as web. El cometido de los taxonomistas no se queda en coordinar y categorizar, pues intervienen en todo el proceso de anÃ¡lisis, gestiÃ³n y preparaciÃ³n para su consulta.
colección como método de conocimiento
Authors: Santolaria Castellanos Ana Isabel , Ramos Alderete Jaime
Published: June 2023

La investigación propone una aproximación a la colección como método mediante el cual se disponen las cosas – objetos o imágenes – “en relación” invitando a que surjan conexiones, muchas veces inesperadas, que construyen el argumento que les da sentido. La colección supone, así, la producción de conexiones y principios que contienen conjeturas, yuxtaposiciones y hallazgos experimentales. Esta forma de adquirir conocimiento es la que plantea Goethe en su colección de colecciones, Aby Warburg con el Atlas Mnemosyne o Quiccheberg en su tratado sobre museos. La colección de objetos o imágenes y su visualización constituye una forma de percibir “las relaciones íntimas y secretas de las cosas, las correspondencias y las analogías”, y provocar un pensamiento activo y propositivo.
THE USAGE OF VIDEO IN TEACHING VOCABULARY FOR YOUNG LEARNERS
Authors: Sabgini Kharisma Naidi Warnanda , Wiraatmaja Triastama
Published: June 2023

Teaching vocabulary to young learners is essential. Therefore, the teacher tries to be creative to attract the young learners’ attention during the learning process. One of the strategies to attract young learners’ attention is using video. Many studies seek the effectiveness of using video to teach vocabulary. However, the studies were mainly conducted quantitatively. Meanwhile, this study is a primary interpretative qualitative study that seeks the usage of video in teaching young learners and the response toward it. The researcher employed human instruments, non-participant observers, and document analysis to achieve triangulation. The data are collected until no new data is forthcoming. Then, the data is analyzed based on the available theory. Based on the data, the student-teachers used video to teach young learners vocabulary as media. The student-teachers employed the video to present, practice, and produce new words. The student-teachers employed the video by active viewing, freeze frame, and reproduction. The young learners’ response toward the usage of video is positive. This research discovered that there had been an active engagement between student-teacher and young learners as well as among your learners.
Estrategias metodológicas de los maestros en coherencia con el modelo pedagógico dialogante de la Institución Educativa Divino Niño Jesús de Cumbal, Nariño
Authors: Bejarano Chamorro Jessica Andrea , Cuaical Taimal Martha Yaqueline , Pastás Quenan Tania Milena
Published: June 2023

Mediante el presente artículo se pretende mostrar los hallazgos más significativos encontrados en la investigación titulada: Estrategias metodológicas que utilizan los docentes en relación con el modelo pedagógico dialogante de la Institución Educativa Divino Niño Jesús del municipio de Cumbal. La investigación se fundamentó bajo el paradigma cualitativo con enfoque hermenéutico y tipo de investigación descriptivo, con el fin de buscar respuestas a la pregunta problema de investigación: ¿cuál es la coherencia entre el modelo pedagógico dialogante y las estrategias metodológicas que utilizan los docentes de la Institución Educativa Divino Niño Jesús, sede N.o 1, municipio de Cumbal, Nariño? Para recolectar la información, se utilizó las siguientes técnicas: el análisis documental, la entrevista semiestructurada a docentes y la observación directa. Con base en los resultados se determinó que los docentes de la institución conocen los aspectos teóricos del modelo dialogante y, con ello, las estrategias metodológicas, pero en la práctica aún distan de responder a las características de este modelo, ya que utilizan estrategias metodológicas enmarcadas en el enfoque heteroestructurante.
Tráfico internacional de crianças e suas consequências no instituto da adoção internacional
Authors: Aizawa Juliana Tomiko Ribeiro , Yotsui Tais Mayumi Ortega
Published: June 2023

O artigo tem por objetivo analisar o crime de tráfico de crianças para fins de adoção ilegal e as consequências dessa prática no instituto da adoção internacional. Dessa forma, será realizada a distinção do instituto da adoção internacional e o tráfico internacional de crianças, bem como a faixa etária tutelada nos documentos jurídicos internacionais e do Brasil. Para tanto, é necessário observar a evolução dos direitos humanos em declarações e convenções, bem como quais as normas vigentes na legislação brasileira para a proteção de crianças e adolescentes. Além do mais, é necessário entender que a adoção internacional é proveniente de um processo jurídico regular formal, para que sejam cessadas as abordagens desacertadas acerca do instituto devido à prática das adoções internacionais ilegais. Por fim, analisaremos alguns precedentes jurisprudenciais para verificar como o ordenamento jurídico brasileiro tem abordado a matéria.
There’s an App for That: Development of a Digital App for Mental Health Issues
Authors: Wathoni Ruhmaya Nida , Santoso Adhi Setyo
Published: June 2023

Mental health deserves greater attention and awareness, as many people are unaware that it encompasses emotional, psychological, and social well-being. These factors are reflected in how individuals think, feel, and act. Taking care of one's mental health is crucial at any stage of life. Additionally, society needs to be more open about discussing stress, anxiety, and depression, and not perceive them as weaknesses or taboo subjects. The increasing prevalence of mental health issues is a consequence of modern existence, particularly the unstoppable growth of technology. While technology has its benefits and can save lives, connect people, and improve lives in various ways, it also contributes to the rising mental health challenges. Recognizing the significance of this issue, Ceritain.id offers a solution. Ceritain.id is a website application designed to assist individuals in need of mental support by providing a platform to share daily stories and access support systems. The website application facilitates counseling sessions with counselors, connecting individuals based on their interests and concerns. The goal of this initiative is to help individuals suffering from mental fatigue find relief from the burdens they carry.
Analisis Pengaruh Inflasi, Produk Domestik Bruto, Pertumbuhan Penduduk Dan Sertifikat Bank Indonesia Syariah Terhadap Harga Saham (Studi Kasus Perusahaan Properti Dan Real Estate Dalam Indeks Saham Syariah Indonesia Tahun 2016-2020)
Authors: Andriani Mutia , Hamid Abdul , Fakhrizal
Published: June 2023

Saham sektor properti dan real estate memiliki ciri tersendiri dari sektor lain dalam bursa. Sektor ini menciptakan dan memenuhi kebutuhan masyarakat yakni tempat tinggal ataupun gedung sehingga menjadikan sektor properti dan real setate sebagai sektor yang menjanjikan. Namun pada kenyataannya, kondisi suatu negara ikut mempengaruhi harga saham properti dan real estate terlebih pada tahun 2019 menyebarnya wabah Covid-19 yang melemahkan perekonomian global tak terkecuali di indonesia yang mengakibatkan permintaan dan penawaran saham pada sektor ini hanya gencar di waktu-waktu tertentu saja. Metode yang digunakan dalam penelitian ini adalah pengumpulan data melalui website dan studi kepustakaan. Total sampel yang diuji sebanyak 10 perusahaan properti dan real estate dalam Indeks Saham Syariah Indonesia tahun 2016-2020 dengan metode purposive sampling. Analisis data dalam penelitian ini menggunakan analisis data panel dengan alat bantu eviews 9. Hasil analisis menunjukkan bahwa secara simultan inflasi, produk domestik bruto, pertumbuhan penduduk dan Sertifikat Bank Indonesia Syariah berpengaruh signifikan terhadap harga saham. Kemudian secara parsial didapati hasil bahwa variabel inflasi berpengaruh negatif dan signifikan dengan nilai probabilitas 0.0464, variabel produk domestik bruto berpengaruh positif dan signifikan dengan nilai probabilitas 0.0000, variabel pertumbuhan penduduk berpengaruh negatif dan tidak signifikan dengan nilai probabilitas 0.4237 dan variabel sertifikat bank Indonesia syariah berpengaruh positif dan signifikan dengan nilai probabilitas 0.000 dengan tingkat kepercayaan sebesar 81,22% sedangkan sisanya sebesar 18,78% dipengaruhi oleh variabel lain yang tidak diteliti dalam penelitian ini.

Kata Kunci: Inflasi, Produk Domestik Bruto, Pertumbuhan penduduk, Sertifikat Bank Indonesia Syariah, Harga Saham Properti.
Analysis of the heart rate variability of the operational-level officers of the Ukrainian Armed Forces
Authors:   The National Defence University of Ukraine named after Ivan Cherniakhovskyi, Petrachkov Oleksandr , Yarmak Olena ,   The National Defence University of Ukraine named after Ivan Cherniakhovskyi
Published: June 2023

the study of the adaptive capabilities of the cardiovascular system of servicemen of the Armed Forces of Ukraine requires a comprehensive study. this issue is relevant in the conditions of the legal regime of martial law. The purpose of the research was to study conditions of the regulatory mechanisms of the vegetativenervous system of servicemen in the conditions of the legal regime of martial law. to solve the defined goal, we conducted a study of a group of operational-level officers (n=201, the average age of the subjects was 36.4 years). the study was conducted using a multifunctional device “mPFI Rhythmograph-1”. Heart rate variability and the obtained results were processed using methods of mathematical statistics. The research’s results of heart rate parameters in operational-level officers were conducted on the basis of time, spectral and autocorrelation indicators. According to the results of the study, it was found that the sympathetic nervous system dominates in military personnel. this indicates a intensification of the autonomous regulation of heart rate. the obtained results of the heart rate variability of the operational-level officersindicate increasing of sympathetic influences, which may be related with increasing level of fatigue. the obtained results of spectral indicators showed the presence of extreme values in the ranges of 75 % percentile. this indicates a violation of metabolic and energy processes in the studied group. the study of the indicators of autocorrelation analysis helped us to obtain additional information about officers’ heart rate. the obtained median result is 0.810 units. this indicates a high degree of correlation between successive heart rate intervals with one interval distance. A correlation analysis was also conducted and it was determined that the median result is 19.0 pairs of intervals. Conclusions. the interpretation of the obtained results can be useful for studying the physiological condition of operational-level officers during combat missions. the results can be used to develop training programs. And this will help to reduce the impact of stress on the servicemen, to increase the adaptive capabilities and to monitor their functional conditions.
ASET PENGHIDUPAN MASYARAKAT DALAM MENCEGAH KEKUMUHAN DI DUSUN DELIKSARI, KOTA SEMARANG
Authors: Medina Novia Cecilia , Sunarti Sunarti
Published: June 2023

Perbaikan permukiman kumuh yang menitikberatkan pada aspek fisik dapat memicu transformasi parsial, sehingga pertimbangan penghidupan penting untuk mencegah kekumuhan. Dusun Deliksari perlu pencegahan kekumuhan karena masyarakat telah berulang kali tinggal di permukiman kumuh karena keterbatasan ekonomi, kerawanan bencana, dan ketergantungan pada bantuan. Penelitian ini bertujuan untuk menganalisis karakteristik aset penghidupan dalam mencegah kekumuhan. Penelitian ini menggunakan pendekatan kuantitatif dengan teknik analisis skoring dari jawaban kuesioner 68 responden. Hasil penelitian menunjukkan bahwa aset penghidupan masyarakat berada pada level sedang dan masih terdapat 5 indikator aset dengan kondisi rendah. Aset ekonomi dengan skor terendah merupakan masalah utama yang mempengaruhi rendahnya kesejahteraan dan masalah aset fisik, seperti ketidakmampuan mengakses perumahan yang layak dan alokasi sumber daya yang tidak merata untuk meningkatkan kualitas infrastruktur. Di sisi lain, kekuatan aset penghidupan terutama keberadaan kelompok masyarakat, tolong menolong, dan kesediaan untuk memperbaiki kerusakan yang disebabkan oleh faktor alam, dapat mendukung pencegahan kekumuhan.
A igreja protestante e a violência doméstica contra as mulheres
Authors: Guerra Kellen Margareth Peres Pamplona , Dendasck Carla Viana Dendasck
Published: June 2023

Existe uma associação antiga e popular da religião com a paz, o sentimento de amor, o cuidado com aqueles indivíduos desfavorecidos. É possível a colaboração dos tabus religiosos com a manutenção das mulheres que são alvo de violência em seus relacionamentos. A ausência de preparo teológico para tratativa de situações envolvendo violência doméstica também é capaz de colaborar e incentivar a perpetuação dos mitos religiosos que condescendem com essa realidade. O trabalho teve como objetivo geral compreender o papel da igreja frente à violência doméstica sofrida pelas mulheres. Para elaboração do trabalho fez-se uma revisão de literatura baseada, principalmente, em livros de diversos autores da área envolvendo a temática proposta. Ainda se realizou um levantamento de informações sobre o tema em revistas, artigos, documentários, relatórios, periódicos, entre outras fontes de dados. Tem-se como resultado o entendimento de que um dos principais desafios da igreja envolve a criação de um panorama histórico, cultural e social baseado na ideia de um líder espiritual, seja ele pastor ou padre, capaz de explicar a violência sofrida e causada, de forma que não influencie de modo direto as mulheres a terem que “passar por cima ou aceitar” a violência sofrida com base no que prega a religião, analisando todas as consequências que possam surgir dessa situação, visto que um conselheiro não deve estar limitado aos ensinamentos bíblicos ou baseando-se apenas na fé. Sendo assim, é possível perceber a ausência, na sociedade, de certo aconselhamento espiritual informatizado e preparado com base na legislação, para lidar com os desafios envolvendo o tema em questão, propiciando uma sensação de real segurança às vítimas de violência doméstica.
Avaliação de medidas cefalométricas angulares para análise da tendência de crescimento vertical e ângulo Z em uma amostra de pacientes com oclusão normal
Authors: Araujo Priscila Pinto Brandão de , Nouer Paulo Roberto Aranha
Published: June 2023

A realização deste trabalho teve como objetivo avaliar cefalometricamente as medidas angulares e características faciais de indivíduos pertencentes ao município do Rio de Janeiro-RJ. Para este estudo, 531 indivíduos do sexo masculino pertencentes às organizações militares do Rio de Janeiro foram submetidos à ficha clínica do curso de Mestrado em Ortodontia do Centro de Pesquisas – São Leopoldo Mandic. Dessa amostra, observou-se que 22 indivíduos possuíam oclusão normal e que nunca haviam se submetido a tratamento ortodôntico. Nesses pacientes foram feitas tomadas de telerradiografias de cabeça em norma lateral e avaliadas as grandezas cefalométricas: Eixo “Y”, NS.Go-Me, SN.PLO, FMA, SN-Go-Gn, ângulo Z, entre outras. Os dados obtidos foram submetidos à análise com nível de significância de 5% (p< 0,05) e utilizadas estatísticas descritivas e o erro de Dahlberg. Os resultados demonstraram que os indivíduos com oclusão normal do município do Rio de Janeiro apresentaram estas características cefalométricas dentro dos padrões de normalidade como já descritos na literatura.
Determining the density of molten Y2O3 using an electrostatic levitation furnace in the International Space Station
Authors: Oda Hirohisa , Shimonishi Rina , Koyama Chihiro , Ito Tsuyoshi , Ishikawa Takehiko
Published: June 2023

This work employed the electrostatic levitation furnace (ELF) apparatus installed in the KIBO Japanese Experiment Module on the International Space Station. The ELF is able to levitate high-melting-point materials such as ceramics without containers. The heating and melting of refractory oxides in this apparatus allows the analysis of various thermophysical properties, including density, surface tension and viscosity, that are otherwise very difficult to measure in terrestrial laboratories. In the present study, the density of molten Y2O3 was determined over a wide temperature range using the ELF. A density of 4700 kg/m3 was obtained at 2712 K, in good agreement with the value previously obtained using an aero-acoustic levitator. The relationship between the ionic radius and the molar volume of liquid Y2O3 was ascertained and found to be similar to those for other non-glass-forming sesquioxides.
The L2 Motivational Self System and L2 Achievement: A Study of DNTU's Students
Authors: Loan Tran Thi Thuy
Published: June 2023

The study detailed in this article investigates the connection between the L2 competency level of Vietnamese English as a Foreign Language (EFL) learners and Dörnyei's (2005, 2009) Second Language Motivational Self System (L2MSS). Descriptive and inferential analyses of the collected data revealed expected—that the components of the L2MSS were a good predictor of the learners’ intended learning efforts. However, the study also established that in this learner population, these components were not consistently correlated with L2 achievement. The findings can be treated as evidence that self-reported motivation does not always have behavioral consequences.
Mechanisms of Rapid Bactericidal and Anti-Biofilm Alpha-Mangostin In Vitro Activity against Staphylococcus aureus
Authors: Deng Xiangbin , Xu Hongbo , Li Duoyun , Chen Jinlian , Yu Zhijian , Deng Qiwen , Li Peiyu , Zheng Jinxin , Zhang Haigang
Published: June 2023

Alpha-mangostin (α-mangostin) was discovered as a potent natural product against Gram-positive bacteria, whereas the underlying molecular mechanisms are still unclear. This study indicated that α-mangostin (at 4 × MIC) rapidly killed Staphylococcus aureus planktonic cells more effectively (at least 2-log10 CFU/ml) than daptomycin, vancomycin and linezolid at 1 and 3 h in the time-killing test. Interestingly, this study also found that a high concentration of α-mangostin (≥4×MIC) significantly reduced established biofilms of S. aureus. There were 58 single nucleotide polymorphisms (SNPs) in α-mangostin nonsensitive S. aureus isolates by whole-genome sequencing, of which 35 SNPs were located on both sides of the sarT gene and 10 SNPs in the sarT gene. A total of 147 proteins with a different abundance were determined by proteomics analysis, of which 91 proteins increased, whereas 56 proteins decreased. The abundance of regulatory proteins SarX and SarZ increased. In contrast, the abundance of SarT and IcaB was significantly reduced (they belonged to SarA family and ica system, associated with the biofilm formation of S. aureus). The abundance of cell membrane proteins VraF and DltC was augmented, but the abundance of cell membrane protein UgtP remarkably decreased. Propidium iodide and DiBaC4(3) staining assay revealed that the fluorescence intensities of DNA and the cell membrane were elevated in the α-mangostin treated S. aureus isolates. In conclusion, this study reveals that α-mangostin was effective against S. aureus planktonic cells by targeting cell membranes. The anti-biofilm effect of α-mangostin may be through inhibiting the function of SarT and IcaB.
Modern methods for the determination of pesticide residues in beekeeping products and for the diagnostics of bee poisoning
Authors: Omelchun Y. , Kobish A.
Published: June 2023

Intensification of agricultural production is associated with the use of a significant amount of pesticides, which negatively affects the environment and human health, and food products, including beekeeping products, accordingly require mandatory control of residual amounts of pesticides. This article provides a comparative analysis of the available chromatographic methods for pesticide residue research. The necessity of using modern chromatographic methods to determine residual amounts of pesticides in samples of dead bees and beekeeping products is well-founded. Chromatographic methods of studying these indicators in different types of matrices are a priority. They are effective methods of analysis, widely used due to their versatility - they allow the analysis of complex inorganic and organic compounds in various aggregate states. But one of the most common modern methods for pesticide determination is gas and liquid three-quadrupole tandem chromatography-mass spectrometry (GC and/or LC-MS/MS). The GC-MS/MS method provides quantitative determination of analytes at a level that is an order of magnitude higher than, for example, the gas single quadrupole mass spectrometry method. Modern methods of gas and liquid chromatography in combination with quadrupoletime-of-flight mass spectrometric detection (LC/Q-TOF/MS or GC/ Q-TOF/MS) also allow qualitative and quantitative multicomponent analysis of pesticides in beekeeping products. GC and LC systems combined with high-resolution Orbitrap MS (GC-HRMS(Q-Orbitrap)/LC-HRMS (Q-Orbitrap)) have higher sensitivity, enabling ultra-trace detection, and are the most sensitive screening method for multicomponent determination of pesticide residues. Thus, the latest chromatographic methods are able to meet the needs of analytical testing and research laboratories in the field of food safety, including beekeeping products.
Key words: chromatographic methods, thin-layer chromatography, gas chromatography, liquid chromatography, mass spectrometry, multi-component analysis, pesticides, honey, dead bees.
The problem of antibiotic resistance of microorganisms in Ukraine and the world
Authors: Chemerovska I. , Rublenko I.
Published: June 2023

Basically, antibiotic resistance develops due to the incorrect use of antibiotics in various branches of animal husbandry, both during the treatment or prevention of diseases, and due to their long-term use as growth stimulants. As a result, costs for the treatment of farm animals and companion animals are increasing. Antibiotic resistance among microorganisms is a threat to every person, every patient, medical and veterinary worker. Also, this is a big challenge for the field of health care, veterinary medicine and agriculture as a whole. It is very difficult to solve the problem of resistance, because it is not one-sided. Medicines that were effective a few years ago are losing their positions today, and their use is being forced to be limited. According to data from the World Health Organization, the rapid increase in the resistance of microorganisms to antibacterial drugs threatens the scientific gains made by scientists during the last 50-70 years. The formation of antibiotic resistance is due to the genetic properties of microorganisms, as a result of their acquisition of new genetic information, or due to a change in the level of expression of the bacterial cell's own genes. An important factor in the fight against the spread of antibiotic resistance is the pharmacodynamic substantiation of the dosing regimens of antibacterial drugs and their use for specific microorganisms.There are guiding documents that control and recommend the reliability of determining the sensitivity of microorganisms to antibiotics, in particular - methodological recommendations of the European organization EUCAST, the data and material of which are periodically (annually) updated. These documents are developed primarily for routine use in clinical laboratories that do not cover technical procedures for identifying resistance mechanisms at the molecular level. However, a significant part of the given data, research on determining the sensitivity of microorganisms to antibiotics, is performed in national reference laboratories. There is a change in the sensitivity of the micro-flora to antibiotics, which is not covered by the screening of multi-resistant microorganisms, or the direct detection of resistance in clinical samples. Therefore, the study of the problem remains relevant and expedient.
Key words: microorganisms, resistance, antibiotics, gram-positive bacteria, gram-negative bacteria, control, disease, spread, problem, treatment, animals.
Evaluation of changes in indicators of the liver and kidneys functional state in sheep under the influence of the drug "Аlphabet for animals"
Authors: Vovkotrub N. , Melnyk A. , Piddubnyak O. , Kharchenko A. , Chub O.
Published: June 2023

The article provides data on the analysis of changes in the metabolic profile of the liver and kidneys during and after the use of a vitamin-amino acid complex containing essential amino acids and biologically active substances, such as vitamins A, D, E, K, B1, B3, B5, B 12. The existing shortage of nutrients and biologically active substances in the diets of sheep prompts scientists and practitioners to conduct a constant search for the use of non-traditional local feeds and additives of a wide variety of origins. An important role in this plan is given to mineral elements, enzymes, amino acids and vitamins. The use of these biologically active nutrients allows the most effective use of nutrients in the diet, which in turn ensures the maximum possible genetically determined productivity of animals, high reproductive capacity. However, these issues are still poorly studied and require fundamental research, specifically in certain regions of the country. So, in the context of the above, there is a need to conduct research related to increasing the transformation of feed nutrients into the products of ewes by optimizing amino acid and vitamin nutrition in order to maximize their productive qualities. In the conducted research, the positive effect of the drug "Аlphabet for animals" on the functional state of the liver and kidneys of ewes was established, since biomarkers that characterize the work of these organs, such as the content of total protein, cholesterol, urea nitrogen, creatinine, the activity of hepatoindicative enzymes, did not show negative changes. on the contrary, they had stabilizing dynamics. The components of the vitamin-amino acid complex had a positive effect on stimulating the albumin-synthesizing function of the liver in sheep.
Key words: sheep, vitamin-amino acid complex, hepatorenal status, protein-lipid metabolism, hepatoindicative enzymes.
Protein metabolism in deep-bodied cows and heifers
Authors: Bilyk B. , Sakhniuk V.
Published: June 2023

Health and performance management systems should focus on early detection and subsequent prevention of physiological imbalances in dairy herds. Therefore, the need for constant monitoring of the state of health of cows of various physiological and technological groups, in particular markers of protein metabolism, is urgent. The content of total protein in blood serum of deep-bodied cows and heifers was established in the range of 58.3–102.7 g/l (77.2± 0.48 g/l). Disorders of its metabolism were diagnosed in 35.4% of animals, and in most of them (20.4%), mainly in heifers, it was manifested by hypoproteinemia. The optimal content of albumins was established in 81.2% of the studied herd with an average value of 41.1± 0.26%, including in 74.6% of dry cows and in 91.7% of heifers. Hypoalbuminemia was diagnosed in an average of 18.8% of animals, including in 25.4% of cows and in 8.3% of heifers. In dry cows, the pathology arose mainly as a result of the development of dystrophic processes in the liver in the previous lactation periods, in heifers, primarily due to a protein deficiency in the diet. In 72.2% of the studied animals, 40–10 days before the expected birth, there are no violations of the colloidal stability of coarsely dispersed proteins. In 14.1% of blood serum samples, the test was weakly positive (++), in 11.5% - positive (+++) and strongly positive (++++). In another 2.2% of cows, the formation of a dense clot of a milky white color in the test tube was ascertained already after 4–7 hours. after the reaction (the test is hyperpositive - +++++). Optimal values of urea metabolism in blood serum were established in 48.1% of dry cows and in 55.0% of heifers (3.53± 0.043 mmol/l; 1.82–6.80). A decrease in its content was diagnosed in 49.7 and 45.0% of animals, respectively. The metabolism of creatinine in blood serum was optimal in 94.8% of the examined dry cows and in 94.1% of the heifers, and its values were within the reference values. Hypercreatininemia was diagnosed in 5.4% of the studied animal population, which can probably be caused by the development of dystrophic processes in the glomeruli of the kidneys.
Key words: metabolism, diagnostics, proteins, albumins, urea, creatinine, liver, deep-bodied cows, heifers.
Increase in willow vegetative weight depends on varietal characteristics and the period of planting material collection
Authors: Danyuk Yu. , Balagura O.
Published: June 2023

The article presents the results of research on the growth of the ground mass of willow depending on varietal characteristics, the time of harvesting the planting material and the use of MaxiMarin absorbent gel. Methods. Field, measuring, mathematical and statistical. The results. The research was conducted with two types of willow, the Panfilska variety and the Zb-ruch rodshaped variety, at the experimental field of the Institute of Bioenergy Crops and Sugar Beet during 2019–2022. It was found that the use of MaxiMarin absorbent gel for planting willow shoots harvested both in autumn and in spring did not ensure increasing their survivability. Survival of shoots harvested in autumn was significantly higher than those harvested in spring, regardless of varietal characteristics. No significant increase in biometric parameters – plant height, shoot thickness, and number of stems – was found, depending on the time of harvesting the planting material and the use of the absorbent gel. The use of absorbent gel for planting shoots harvested in the fall of the Zbruch variety ensured a reliable increase in stems, compared to both the control – without gel, and the harvesting of planting material in the spring of both varieties. In the first year of vegetation, on an average of three years, there was a tendency to increase the growth of the willow height and the thickness of the stems of the rodshaped variety Zbruch, compared to the three-stemmed willow of the Panfilska variety, and in the last recording dates, on the contrary, the growth of the height and thickness of the stems of the Panfilska variety was greater than that of the Zbruch variety regardless of the period of harvesting shoots. The intensity of growth of the vegetative mass of the willow depends on what the yield of planting material – cuttings or shoots depends on – both its height and the diameter of the stems and their number.
The height of the plants and the thickness of the willow stems in dynamics depended on varietal characteristics. Growth of vegetative mass was more intense in rod-shaped willow of the Zbruch variety than in the threestamen variety of Panfilska. The use of MaxiMarin absorbent gel for planting willow shoots, which were harvested both in autumn and in spring, did not increase their survival rate.
Key words: variety, shoots, cuttings, height, diameter of plants, number of stems.
Tác động của đầu tư trực tiếp nước ngoài đối với năng suất các nhân tố tổng hợp của các doanh nghiệp công nghiệp hỗ trợ nội địa
Authors: Nguyễn Quỳnh Trang , Tô Trung Thành
Published: June 2023

Tác động của đầu tư trực tiếp nước ngoài (FDI) tới doanh nghiệp công nghiệp hỗ trợ nội địa đóng vai trò quan trọng đối với việc thúc đẩy tham gia chuỗi giá trị toàn cầu của nền kinh tế. Để đánh giá tác động của FDI đối với năng suất nhân tố tổng hợp (TFP) của công nghiệp hỗ trợ nội địa, bài báo nghiên cứu hai kênh tác động: nội ngành và hạ nguồn. Biến tương tác giữa FDI và hai yếu tố: chất lượng nhân lực và cường độ vốn cũng được xem xét để đánh giá khả năng hấp thụ tác động lan tỏa từ FDI. Sử dụng dữ liệu điều tra doanh nghiệp 5 năm (2014-2018), sau khi ước lượng TFP, nghiên cứu sử dụng mô hình dữ liệu bảng tác động ngẫu nhiên để đánh giá tác động này. Kết quả cho thấy các doanh nghiệp FDI hạ nguồn có tác động tích cực, trong khi các doanh nghiệp FDI nội ngành gây ra tác động tiêu cực; các doanh nghiệp nội địa có nhân lực chất lượng cao hơn sẽ có khả năng hấp thụ tác động hạ nguồn tốt hơn.
The Optimal Time between Embolization and Surgery for Hypervascular Spinal Metastatic Tumors : A Systematic Review and Meta-Analysis
Authors: Yuh Woon Tak , Han Junghoon , Lee Chang-Hyun , Kim Chi Heon , Kang Hyun-Seung , Chung Chun Kee
Published: June 2023

Objective : Preoperative transarterial embolization (TAE) of tumor feeders in hypervascular spine metastasis is known to reduce intraoperative estimated blood loss (EBL) during surgery. The effect of TAE varies for several reasons, and one controllable factor is the timing between embolization and surgery. However, the adequate timing remains unclear. This study aimed to evaluate the timing and other factors that reduce EBL in spinal metastasis surgery through a meta-analysis.Methods : A comprehensive database search was performed to identify direct comparative studies of EBL stratified by the timing of surgery after TAE for spinal metastasis. EBL was analyzed according to the timing of surgery and other factors. Subgroup analyses were also performed. The difference in EBL was calculated as the mean difference (MD) and 95% confidence interval (CI).Results : Among seven studies, 196 and 194 patients underwent early and late surgery after TAE, respectively. The early surgery was defined as within 1–2 days after TAE, while the late surgery group received surgery later. Overall, the MD in EBL was not different according to the timing of surgery (MD, 86.3 mL; 95% CI, -95.5 to 268.1 mL; p=0.35). A subgroup analysis of the complete embolization group demonstrated that patients who underwent early surgery within 24 hours after TAE had significantly less bleeding (MD, 233.3 mL; 95% CI, 76.0 to 390.5 mL; p=0.004). In cases of partial embolization, EBL was not significantly different regardless of the time interval.Conclusion : Complete embolization followed by early spinal surgery within 24 hours may reduce intraoperative bleeding for the patients with hypervascular spinal metastasis.
Comparison between Placement of an Amniotic Membrane Contact Lens and Temporary Amniotic Membrane Transplantation
Authors: Choi Soo Young , Choi Jae Won , Park Woo Chan
Published: June 2023

Purpose: We compared the clinical utilities of an amniotic contact lens and temporary amniotic membrane transplantation in patients with persistent corneal epithelial defects.Methods: Patients diagnosed with persistent corneal epithelial defects from November 2019 to July 2022 were randomly assigned to either lens placement for 2 weeks or temporary transplantation for 1 week, and the outcomes were retrospectively analyzed. The best-corrected visual acuity, corneal sensation, tear film break-up time, and corneal opacity were measured before and after the interventions. The treatment success rates, recurrences, and other complications were analyzed during the follow-up period.Results: We placed lenses in 32 patients (32 eyes) and 20 patients (20 eyes) underwent transplantation. In both groups, significant differences in best-corrected visual acuity, corneal sensation, tear film break-up time, and corneal opacity were apparent after intervention vs. before. None of the parameters showed any significant differences in terms of their rates of change.Conclusions: In patients with persistent corneal epithelial defects, amniotic contact lens placement is an effective outpatient procedure and is not inferior to temporary amniotic membrane transplantation.
Four-port Vitrectomy in Rhegmatogenous Retinal Detachment
Authors: Heo Jeong Woo , Kwon Yoon Hyung
Published: June 2023

Purpose: The study investigated the results of direct surgical scleral depression using four ports and the chandelier lighting system in rhegmatogenous retinal detachment.Methods: Anatomical and functional success rates and complications were analyzed in 179 eyes diagnosed with rhegmatogenous retinal detachment that underwent primary vitrectomy using four ports at our hospital between March 2012 and February 2022.Results: A total of 168 (93.9%) eyes had anatomical success without reoperation while 11 (6.1%) developed recurrence. However, the final success rate was 100%. The best-corrected visual acuity improved significantly from logarithm of the minimum angle of resolution (logMAR) 1.03 ± 0.92 to logMAR 0.24 ± 0.39 after surgery (p < 0.001). Complications included a temporary increase in the intraocular pressure (n = 3, 1.7%), leakage through the scleral incision (n = 2, 1.1%), hyphema and vitreous hemorrhage (n = 10, 5.6%), and iatrogenic retinal break (n = 1, 0.6%), but these improved after treatment.Conclusions: Four-port vitrectomy for rhegmatogenous retinal detachment in which scleral depression is directly performed using the chandelier lighting system is a useful surgical method. It allows effective visualization of the peripheral retina regardless of the assistant’s proficiency which results in a more meticulous surgery. The anatomical and functional results of this technique were similar to the conventional technique.
Effects of Vascular Endothelial Growth Factor on YAP/TAZ Signaling in Trabecular Meshwork Cells
Authors: Kim So Young , Sung Mi Sun , Park Sang Woo
Published: June 2023

Purpose: To investigate the effects of vascular endothelial growth factor (VEGF) on yes-associated protein (YAP)/transcriptional coactivator with a PDZ-binding motif (TAZ), a Hippo pathway-related transcription factor, and the role of YAP/TAZ induced by trabecular meshwork stimulation.Methods: Human trabecular meshwork cells were cultured and treated with various VEGF concentrations to verify cell cytotoxicity using the CCK-8 solution. Transforming growth factor β-2 (TGFβ2; 5 ng/mL) and VEGF (30 ng/mL) were applied and YAP/TAZ expression was assessed by western blotting, reverse transcription quantitative polymerase chain reaction and immunocytochemistry. Fibronectin, collagen 1, and myocilin expression were also assessed by western blotting. The cells were stained using Alexa Fluor 488-phalloidin to observe F-actin changes.Results: YAP and TAZ expression increased following TGFβ2 and VEGF treatment for 24 hours. Fibronectin and collagen 1 increased significantly in all three treatment groups, while myocilin increased in the TGFβ2 and TGFβ2+VEGF groups. The F-actin staining showed increased cross-linking in the trabecular meshwork cells.Conclusions: VEGF induced YAP/TAZ signaling and increased trabecular meshwork cell fibrosis. Based on the functional changes caused by VEGF, it is suggested that VEGF and YAP/TAZ may increase aqueous humor outflow resistance in trabecular meshwork cells.
Implementing Online Tools to the Pre-writing Stage: An Investigation into Online Writing Classes
Authors: Bui Diem Bich Huyen , Vu Tien Thinh
Published: June 2023

Writing is considered one of the most challenging skills for second and/ or foreign-language learners, particularly in the online environment. Numerous researchers worldwide have explored how to stimulate students' schemata and develop their writing skills. This study critically investigates the contribution of implementing online tools, such as Padlet and Jamboard, during the pre-writing stage to enhance students' writing abilities and their reactions to this application. A seven-week experimental research design was conducted with two online writing classes using the Zoom platform. The participants were 62 pre-intermediate students in the Intensive English program. T-tests were used to analyse the data, and a survey was administered to students in the experimental group at the end of week 7 to gather their perceptions. The findings revealed that integrating Padlet and Jamboard positively impacted the organisation and content of students' written texts. The survey results showed that most students were satisfied with the effectiveness of the lessons and their level of engagement in class. This study highlights the potential of technology integration in teaching writing, especially during the pre-writing stage, and suggests further research in this area.