bluuebunny/crossref_metadata_2025 huggingface dataset:
Haploidy in Tobacco Induced by PsASGR-BBML Transgenes via Parthenogenesis
Authors: Zhang Zhifen , Conner Joann , Guo Yinping , Ozias-Akins Peggy
Published: September 2020

Background: Engineering apomixis in sexually reproducing plants has been long desired because of the potential to fix hybrid vigor. Validating the functionality of genes originated from apomictic species that contribute to apomixis upon transfer to sexually reproducing species is an important step. The PsASGR-BABYBOOM-like (PsASGR-BBML) gene from Pennisetum squamulatum confers parthenogenesis in this apomict, and its functionality was demonstrated in several sexually reproducing monocots but not in any dicots. Methods: We introduced the PsASGR-BBML gene regulated by egg cell-specific promoters, either AtDD45 or AtRKD2, into tobacco, and analyzed progeny of the transgenic lines resulting from self-pollination and crossing by flow cytometry. Results: We identified haploid progeny at a frequency lower than 1% in the AtDD45pro lines, while at a frequency of 9.3% for an octoploid (2n = 8x) AtRKD2pro line. Haploid production in the T2 generation, derived from the tetraploid T1 offspring of this original octoploid AtRKD2pro line, was also observed. Pollinated by homozygous transgenic tobacco carrying a DsRed marker gene, 4x progeny of the AtRKD2pro line yielded parthenogenetic embryos identified as DsRed negative. We verified that the DsRed negative seedlings recovered were haploid (2x). Conclusion: The PsASGR-BBML gene regulated by egg cell-specific promoters could enable parthenogenesis in tobacco, a dicotyledon species.
Identification and Characterization of Three Heat Shock Protein 90 (Hsp90) Homologs in the Brown Planthopper
Authors: Chen Xuan , Li Ze-Dong , Dai Yi-Ting , Jiang Ming-Xing , Zhang Chuan-Xi
Published: September 2020

Hsp90 (heat shock protein 90) chaperone machinery is considered to be a key regulator of proteostasis under both physiological and stress growth conditions in eukaryotic cells. The high conservation of both the sequence and function of Hsp90 allows for the utilization of various species to explore new phenotypes and mechanisms. In this study, three Hsp90 homologs were identified in the brown planthopper (BPH), Nilaparvata lugens: cytosolic NlHsp90, endoplasmic reticulum (ER) NlGRP94 and mitochondrial NlTRAP1. Sequence analysis and phylogenetic construction showed that these proteins belonged to distinct classes consistent with the predicted localization and suggested an evolutionary relationship between NlTRAP1 and bacterial HtpG (high-temperature protein G). Temporospatial expression analyses showed that NlHsp90 was inducible under heat stress throughout the developmental stage, while NlGRP94 was only induced at the egg stage. All three genes had a significantly high transcript level in the ovary. The RNA interference-mediated knockdown of NlHsp90 its essential role in nymph development and oogenesis under physiological conditions. NlGRP94 was also required during the early developmental stage and played a crucial role in oogenesis, fecundity and late embryogenesis. Notably, we first found that NlHsp90 and NlGRP94 were likely involved in the cuticle structure of female BPH. Together, our research revealed multifunctional roles of Hsp90s in the BPH.
MicroRNAs Regulate Intestinal Immunity and Gut Microbiota for Gastrointestinal Health: A Comprehensive Review
Authors: Bi Kefan , Zhang Xujun , Chen Wenbiao , Diao Hongyan
Published: September 2020

MicroRNAs are small non-coding RNAs regulating gene expression at the post-transcriptional level. The regulation of microRNA expression in the gut intestine is gradually recognized as one of the crucial contributors of intestinal homeostasis and overall health. Recent studies indicated that both the microRNAs endogenous in the gut intestine and exogenous from diets could play influential roles in modulating microbial colonization and intestinal immunity. In this review, we discuss the biological functions of microRNAs in regulating intestinal homeostasis by modulating intestinal immune responses and gut microbiota. We particularly focus on addressing the microRNA-dependent communication and interactions among microRNA, gut microbiota, and intestinal immune system. Besides, we also summarize the roles of diet-derived microRNAs in host-microbiome homeostasis and their benefits on intestinal health. A better understanding of the relationships among intestinal disorders, microRNAs, and other factors influencing intestinal health can facilitate the application of microRNA-based therapeutics for gastrointestinal diseases.
Quantitative Analysis of Pore Space Structure in Dry and Wet Soil by Integral Geometry Methods
Authors: Ivonin Dmitriy , Kalnin Timofey , Grachev Eugene , Shein Evgeny
Published: September 2020

We present a methodology for a numerical analysis of three-dimensional tomographic images in this paper. The methodology is based on integral geometry, topology, and morphological analysis methods. It involves calculating cumulative and non-cumulative pore size distributions of Minkowski functionals and Betti numbers. We investigated 13 samples in dry and wet (saturated beyond the field capacity) conditions within different horizons of the Phaeozem albic. For samples of the arable horizon, an increase in the Euler characteristic was observed in the process of wetting. For samples from the A2, AB and B2 horizons, the Euler-Poincare characteristic decreased during wetting. It has been proven that both Betti numbers (number of isolated pores and number of “tunnels”) decrease with swelling of the AB and B2 horizons at a depth of 20–90 cm. For samples from the arable horizon, another dependence was observed: A Betti number of zero increased first but decreased during wetting. Based on the change in topological characteristics, two methods of changing the topology of the void space of the soil were demonstrated. The above-described quantitative changes of proposed parameters of pore space tomographic images prove the possibility and progressiveness of their usage for the pore space transformation estimate.
A Tornado Hitting the Homeland: Disturbing American Foundational Myths in Bharati Mukherjee’s Jasmine
Authors: Seeliger Henriette-Juliane
Published: September 2020

Historically, the United States has always been a country of immigration. Yet, in light of recent political events, a form of nativism and sedentarism is re-emerging that seeks to preserve what is generally perceived as essentially American: an ethnically white and male identity that has its origins in the foundational myths of the pastoral, the frontier, and the West. The American Midwest is where the allegedly “real” America lies: it is what Anthony D. Smith has termed an 2ethnoscape”: a landscape imbued with historical and cultural meaning that has come to represent true “Americanness”. In her 1989 novel Jasmine, Bharati Mukherjee uses the figure of Jasmine, an undocumented female immigrant from India, to disrupt this traditional trope of “the West” as the perceived location of American cultural identity. She liberates the land from its national, historical, and ethnic inscriptions by subverting the very foundational myths of the pastoral, the frontier, manifest destiny, virgin land, and the melting-pot, that are so crucial to the justification of this exclusive as well as exclusionary identity… This article analyzes the processes and mechanisms through which Mukherjee liberates the landscape: Firstly, she satirizes the ideal of the American pastoral and exposes the assumption of a stable, uniquely American landscape as purely imaginative. She then subverts the notion of the global city as the ideal location of immigrants, where “the other” can be safely contained outside the homeland and instead makes the Midwest ethnoscape the space where her protagonist uproots American national identity. Through her presence in the American heartland, Jasmine disturbs and challenges naturalized notions of America and constructs a new homeland that is open for all immigrants following her. Mukherjee thus shifts the perspective away from seeing the American homeland as a pre-existing place in need of defense, and proposes a fluid understanding of home that has acquired new relevance in light of recent political events.
Non-Communicable Diseases-Related Stigma: A Mixed-Methods Systematic Review
Authors: Rai Sarju Sing , Syurina Elena V. , Peters Ruth M. H. , Putri Annisa Ika , Zweekhorst Marjolein B. M.
Published: September 2020

This systematic review examines and consolidates existing evidence on stigma associated with the top four non-communicable diseases (NCDs)—cancers, cardiovascular diseases, chronic respiratory diseases, and diabetes—and its impact on the lives of people affected. We conducted a systematic literature search in PubMed, PsycINFO, JSTOR, Science Direct, and Web of Science for original research in English that explored health-related stigma among people living with either of the four NCDs. A three-step integrative synthesis of data was conducted. Twenty-six articles (qualitative = 15; quantitative = 11) were selected, with most (n = 15) related to cancers, followed by diabetes (n = 7), chronic respiratory diseases (n = 3), and cardiovascular diseases (n = 1). Blame, shame, and fear were the main causes of stigma, the origin and nature of which differed according to the disease-specific features. The manifestations (enacted and felt stigma) and consequences (social, behavioral, psychological, and medical) of stigma across NCDs were similar. Inconsistencies existed in the conceptualization of stigma processes. To fill this gap, we developed an NCD-related stigma framework. People living with NCDs can experience stigma, which can negatively impact their health, management of their disease, and quality of life. The new framework can help in improving the understanding of the processes and experiences of stigma related to NCDs.
Are Respiratory Responses to Cold Air Exercise Different in Females Compared to Males? Implications for Exercise in Cold Air Environments
Authors: Kennedy Michael D. , Lenz Elisabeth , Niedermeier Martin , Faulhaber Martin
Published: September 2020

Research has shown that cold air exercise causes significant respiratory dysfunction, especially in female athletes. However, how female and male athletes respond to cold air exercise is not known. Thus, we aimed to compare acute respiratory responses (function, recovery and symptoms) in males and females after high-intensity cold air exercise. Eighteen (nine female) athletes completed two environmental chamber running trials at 0 °C and −20 °C (humidity 34 ± 5%) on different days in a randomized starting order. Spirometry was performed pre, 3, 6, 10, 15 and 20 min post. Respiratory symptoms were measured posttrial and heart rate and rating of perceived exertion were assessed during each trial. No significant differences in delta change (pre to post) were found at either temperature between sexes for FEV1, FVC, FEF50% and FEF25–75%. At −20 °C, FEV1 decreased similarly in both sexes (males: 7.5%, females: 6.3%) but not at 0 °C, p = 0.003. Postexertion respiratory function recovery and reported symptoms were not different between sexes at either temperature. These results indicate no sex-based differences in acute respiratory responses (function, recovery and symptoms) to cold air exercise. However, intense exercise at −20 °C is challenging to the respiratory system in both sexes and may lead to altered respiratory responses compared to mild winter conditions like 0 °C.
Place Effects and Chronic Disease Rates in a Rural State: Evidence from a Triangulation of Methods
Authors: Kariburyo Mohamed Shabani , Andress Lauri , Collins Alan , Kinder Paul
Published: September 2020

High rates of chronic diseases and increasing nutritional polarization between different income groups in the United States are issues of concern to policymakers and public health officials. Spatial differences in access to food are mainly blamed as the cause for these nutritional inequalities. This study first detected hot and cold spots of food providers in West Virginia and then used those places in a quasi-experimental method (entropy balancing) to study the effects of those places on diabetes and obesity rates. We found that although hot spots have lower rates of chronic diseases than non-hot spots and cold spots have higher rates of chronic diseases than non-cold spots—the situation is complicated. With the findings of income induced chronic disease rates in urban areas, where most hot spots are located, there is evidence of another case for "food swamps." However, in cold spots which are located mainly in rural areas, higher rates of chronic diseases are attributed to a combination of access to food providers along with lacking the means (i.e., income for low-income households) to form healthier habits.
Can Water Rights Trading Scheme Promote Regional Water Conservation in China? Evidence from a Time-Varying DID Analysis
Authors: Fang Lin , Wu Fengping
Published: September 2020

Using the panel data of 30 provinces in China from 1998 to 2017, we adopt a time-varying difference-in-differences (time-varying DID) model to estimate the impact of water rights trading scheme on regional water consumption. The results show that water rights trading can significantly promote water conservation in the pilot regions by 3.1% compared to that in the non-pilot regions, and a series of robustness tests show consistent results. Policy effects are mainly driven by improving water-use efficiency and adjusting water structure; that is, by transferring water resources from the agricultural sector to the other sectors, agricultural water efficiency is improved and water conflict among sectors is alleviated; thus, water saving is achieved. In addition, by constructing two indexes of regional water pressure and tradable water resources, our heterogeneity analysis shows that water rights trading performs better in areas with high water pressure and large tradable water resources. Under the high pressure of large water use and low water endowment, water rights trading will evidently reduce water consumption more so than in the low-pressure regions, and with water rights trading, it is hard to achieve a policy effect in regions without sufficient tradable water resources. This paper provides important policy implications for China for further promoting the water rights trading scheme in the field of resource conservation.
Structural and Social Determinants of Health Factors Associated with County-Level Variation in Non-Adherence to Antihypertensive Medication Treatment
Authors: Donneyong Macarius M. , Chang Teng-Jen , Jackson John W. , Langston Michael A. , Juarez Paul D. , Sealy-Jefferson Shawnita , Lu Bo , Im Wansoo , Valdez R. Burciaga , Way Baldwin M. , Colen Cynthia , Fischer Michael A. , Salsberry Pamela , Bridges John F.P. , Hood Darryl B.
Published: September 2020

Background: Non-adherence to antihypertensive medication treatment (AHM) is a complex health behavior with determinants that extend beyond the individual patient. The structural and social determinants of health (SDH) that predispose populations to ill health and unhealthy behaviors could be potential barriers to long-term adherence to AHM. However, the role of SDH in AHM non-adherence has been understudied. Therefore, we aimed to define and identify the SDH factors associated with non-adherence to AHM and to quantify the variation in county-level non-adherence to AHM explained by these factors. Methods: Two cross-sectional datasets, the Centers for Disease Control and Prevention (CDC) Atlas of Heart Disease and Stroke (2014–2016 cycle) and the 2016 County Health Rankings (CHR), were linked to create an analytic dataset. Contextual SDH variables were extracted from the CDC-CHR linked dataset. County-level prevalence of AHM non-adherence, based on Medicare fee-for-service beneficiaries’ claims data, was extracted from the CDC Atlas dataset. The CDC measured AHM non-adherence as the proportion of days covered (PDC) with AHM during a 365 day period for Medicare Part D beneficiaries and aggregated these measures at the county level. We applied confirmatory factor analysis (CFA) to identify the constructs of social determinants of AHM non-adherence. AHM non-adherence variation and its social determinants were measured with structural equation models. Results: Among 3000 counties in the U.S., the weighted mean prevalence of AHM non-adherence (PDC < 80%) in 2015 was 25.0%, with a standard deviation (SD) of 18.8%. AHM non-adherence was directly associated with poverty/food insecurity (β = 0.31, P-value < 0.001) and weak social supports (β = 0.27, P-value < 0.001), but inversely with healthy built environment (β = −0.10, P-value = 0.02). These three constructs explained one-third (R2 = 30.0%) of the variation in county-level AHM non-adherence. Conclusion: AHM non-adherence varies by geographical location, one-third of which is explained by contextual SDH factors including poverty/food insecurity, weak social supports and healthy built environments.
The Effect of China’s Health Insurance on the Labor Supply of Middle-aged and Elderly Farmers
Authors: Liu Lingchen , Sun Renji , Gu Yan , Ho Kung Cheng
Published: September 2020

Social security primarily improves residents’ welfare and ensures labor market sustainability. This study presents a new view of the association between health insurance and labor supply by using data from the China Health and Retirement Longitudinal Study. The results reveal that the health insurance system has a remarkable effect on labor supply. The health insurance coverage tends to encourage middle-aged and elderly farmers to increase their farm labor participation rate and working time, especially for their household agricultural labor participation rate and working time. However, it also reduces the non-farm labor participation rate and working time. Different types of health insurance have diverse effects on labor supply. The new cooperative medical insurance has a stronger pull-back effect. It encourages the middle-aged and elderly farmers to leave the urban non-farm sector and transfer to rural areas to engage in their household agricultural work. The urban employee medical insurance encourages farmers to reduce self-employed labor supply and increase employed work. The supplemental health insurance tends to reduce the labor supply of farm employed and non-farm labor supply, but improve the farm labor supply. Furthermore, urban resident medical insurance and government medical insurance encourage farmers to quit directly from the labor market. In conclusion, the health insurance system is facilitating change in the labor market. Policy-makers should pay full attention to such impacts while improving the health insurance system’s design and operation in China.
Overview of Current Advances in Extrusion Bioprinting for Skin Applications
Authors: Perez-Valle Arantza , Del Amo Cristina , Andia Isabel
Published: September 2020

Bioprinting technologies, which have the ability to combine various human cell phenotypes, signaling proteins, extracellular matrix components, and other scaffold-like biomaterials, are currently being exploited for the fabrication of human skin in regenerative medicine. We performed a systematic review to appraise the latest advances in 3D bioprinting for skin applications, describing the main cell phenotypes, signaling proteins, and bioinks used in extrusion platforms. To understand the current limitations of this technology for skin bioprinting, we briefly address the relevant aspects of skin biology. This field is in the early stage of development, and reported research on extrusion bioprinting for skin applications has shown moderate progress. We have identified two major trends. First, the biomimetic approach uses cell-laden natural polymers, including fibrinogen, decellularized extracellular matrix, and collagen. Second, the material engineering line of research, which is focused on the optimization of printable biomaterials that expedite the manufacturing process, mainly involves chemically functionalized polymers and reinforcement strategies through molecular blending and postprinting interventions, i.e., ionic, covalent, or light entanglement, to enhance the mechanical properties of the construct and facilitate layer-by-layer deposition. Skin constructs manufactured using the biomimetic approach have reached a higher level of complexity in biological terms, including up to five different cell phenotypes and mirroring the epidermis, dermis and hypodermis. The confluence of the two perspectives, representing interdisciplinary inputs, is required for further advancement toward the future translation of extrusion bioprinting and to meet the urgent clinical demand for skin equivalents.
Mobile Phone in the Lives of Young People of Rural Mountainous Areas of Gilgit-Baltistan, Pakistan: Challenges and Opportunities
Authors: Rahim Sabit , Sahar Gul , Jabeen Gul , Aman Shah Akber , Jahan Musrat , Bibi Tehmina
Published: September 2020

This research aims to investigate the impact of mobile phones in the lives of youths of mountainous rural areas of Gilgit-Baltistan (GB). A total of 272 (133 male and 139 female) respondents of ages between 16 and 25 years participated in this study. To analyze the demographic data such as age, gender, district, the descriptive statistics (mean, SD and percentage) and inferential statistics such as independent sample t-test were used. The regression analysis was used to analyze the relationship between independent and dependent variables such as mobile phone features (M = 3.66, SD = 1.15); a mobile phone as a tool for socio-economic impact (M = 3.80, SD = 1.20); as a fashion symbol (M = 1.29, SD = 0.11) and a tool for safety (M = 3.91, SD = 1.06). The findings show that 97% (M = 1.026 SD = 0.159) of youths from GB own a mobile phone (47% male and 48% female). The findings also verify that a mobile phone is beneficial to its users in the fields of economic, education, safety, and security. However, using a mobile phone as status symbol could have a negative impact on the lives of youths. This study recommends that the government should develop effective and efficient policy for mobile phone usage and users should also be aware of the blessings and risks associated with using a mobile phone in their lives.
Diapause Regulation in Newly Invaded Environments: Termination Timing Allows Matching Novel Climatic Constraints in the Box Tree Moth, Cydalima perspectalis (Lepidoptera: Crambidae)
Authors: Poitou Laura , Bras Audrey , Pineau Patrick , Lorme Philippe , Roques Alain , Rousselet Jérôme , Auger-Rozenberg Marie-Anne , Laparie Mathieu
Published: September 2020

The association between indirect environmental cues that modulate insect diapause and the actual stressors is by no means granted when a species encounters new environments. The box tree moth, Cydalima perspectalis, is an Asian pest whose rapid invasion in Europe causes considerable economic and ecological impacts. Larvae enter a winter diapause induced by the photoperiod in both native and invaded ranges, but factors that trigger the return to an active phase are still unknown. Yet, identifying them is crucial to understand how diapause end synchronizes with the end of the winter stress encountered in Europe. To test whether activity resumption is regulated by thermal and/or photoperiodic thresholds, or additive effects between these factors often involved in diapause termination, diapausing caterpillars from an invaded area were exposed to crossed treatments at the laboratory. The evolution of diapause rate was monitored over time and compared to that of nearby field sites invaded. A strong positive effect of increasing temperature was found on the rate and dynamics of diapause termination, whereas no compelling effect of photoperiod appeared. Resuming development directly when main stressors fade, not in response to indirect photoperiodic cues that could be mismatched outside native areas, likely contributes to the good match observed between diapause and the new climates that this pest encountered in the invaded range.
Selective Apheresis of C-Reactive Protein for Treatment of Indications with Elevated CRP Concentrations
Authors: Kayser Stefan , Brunner Patrizia , Althaus Katharina , Dorst Johannes , Sheriff Ahmed
Published: September 2020

Almost every kind of inflammation in the human body is accompanied by rising C-reactive protein (CRP) concentrations. This can include bacterial and viral infection, chronic inflammation and so-called sterile inflammation triggered by (internal) acute tissue injury. CRP is part of the ancient humoral immune response and secreted into the circulation by the liver upon respective stimuli. Its main immunological functions are the opsonization of biological particles (bacteria and dead or dying cells) for their clearance by macrophages and the activation of the classical complement pathway. This not only helps to eliminate pathogens and dead cells, which is very useful in any case, but unfortunately also to remove only slightly damaged or inactive human cells that may potentially regenerate with more CRP-free time. CRP action severely aggravates the extent of tissue damage during the acute phase response after an acute injury and therefore negatively affects clinical outcome. CRP is therefore a promising therapeutic target to rescue energy-deprived tissue either caused by ischemic injury (e.g., myocardial infarction and stroke) or by an overcompensating immune reaction occurring in acute inflammation (e.g., pancreatitis) or systemic inflammatory response syndrome (SIRS; e.g., after transplantation or surgery). Selective CRP apheresis can remove circulating CRP safely and efficiently. We explain the pathophysiological reasoning behind therapeutic CRP apheresis and summarize the broad span of indications in which its application could be beneficial with a focus on ischemic stroke as well as the results of this therapeutic approach after myocardial infarction.
Effect of a Combination of Myo-Inositol, Alpha-Lipoic Acid, and Folic Acid on Oocyte Morphology and Embryo Morphokinetics in non-PCOS Overweight/Obese Patients Undergoing IVF: A Pilot, Prospective, Randomized Study
Authors: Canosa Stefano , Paschero Carlotta , Carosso Andrea , Leoncini Sara , Mercaldo Noemi , Gennarelli Gianluca , Benedetto Chiara , Revelli Alberto
Published: September 2020

Herein we aimed at assessing whether Myo-Inositol (MI), Alpha–Lipoic acid (ALA), and Folic acid (FA) could improve oocyte quality and embryo development in non-PCOS overweight/obese women undergoing IVF. Three hundred and twenty-four mature oocytes were obtained from non-PCOS overweight/obese patients, randomized to receive either MI, ALA, and FA (MI + ALA + FA group, n = 155 oocytes) or FA alone (FA-only group, n = 169 oocytes). Oocytes were examined using Polarized Light Microscopy to assess morphological features of zona pellucida (ZP) and meiotic spindle (MS). One hundred and seventy-six embryos (n = 84 in the MI + ALA + FA group, n = 92 in the FA-only group) were assessed by conventional morphology on days 2 and 5, as well as using the Time-Lapse System morphokinetic analysis. A significantly higher ZP retardance, area, and thickness (p < 0.05), and a shorter MS axis (p < 0.05) were observed in the MI + ALA + FA group, suggesting a positive effect on oocyte quality. Conventional morphology evaluation on day 2 showed a higher mean embryo score in the MI + ALA + FA group, whereas embryo morphokinetic was comparable in the two groups. Overall, our data show a possible beneficial effect of the combination of MI, ALA, and FA on oocyte and embryo morphology, encouraging testing of this combination in adequately powered randomized trials to assess their impact of clinical IVF results.
Cystatin C, a Controversial Biomarker in Hypothyroid Patients under Levothyroxine Therapy: THYRenal, a Pilot Cohort Observational Study
Authors: Greco Marta , Foti Daniela Patrizia , Aversa Antonio , Fuiano Giorgio , Brunetti Antonio , Simeoni Mariadelina
Published: September 2020

Background: Cystatin C (Cys-C) is recognized as one of the most reliable renal function parameters in the general population, although it might be biased by thyroid status. Herein, we tested Cys-C and conventional renal parameters in a cohort of hypothyroid patients treated with Levothyroxine. Methods: Eighty-four hypothyroid patients were recruited and subgrouped according to their serum thyroid-stimulating hormone (TSH) values as a paradigm for therapeutic targeting (n = 54, optimal TSH range = 0.5–2 µIU/mL; n = 30, TSH > 2µIU/mL). Serum Cys-C, creatinine, measured and estimated glomerular filtration rates (mGFR and eGFR) were assessed. Results—mGFR and eGFR were comparable among the two subgroups, whereas Cys-C was significantly higher in patients with suboptimal TSH values (>2 µIU/mL) (p < 0.0001). TSH significantly correlated with Cys-C in the overall patient group, and in the subgroup with TSH above the target value (>2 µIU/mL). Out of 20 patients with abnormal Cys-C, 19 had suboptimal TSH levels. Receiver operating characteristic (ROC) analysis indicated Cys-C as a moderately accurate diagnostic tool (AUC = 0.871) to assess Levothyroxine replacement efficacy in hypothyroid patients (63% sensitivity, and 98% specificity). Conclusions: The observation of increased serum Cys-C in patients with suboptimal TSH would suggest the importance of a careful interpretation by clinicians of this biomarker in the case of hypothyroid patients.
Colchicine to Weather the Cytokine Storm in Hospitalized Patients with COVID-19
Authors: Brunetti Luigi , Diawara Oumou , Tsai Andrew , Firestein Bonnie L. , Nahass Ronald G. , Poiani George , Schlesinger Naomi
Published: September 2020

The repurposing of colchicine for the treatment of COVID-19 was suggested based in its immunomodulatory, anti-inflammatory, and anti-viral properties. We performed a single-center propensity score matched cohort study, including all consecutive COVID-19 patients admitted to a community hospital between 1 March 2020 and 30 May 2020. Patients were stratified according to the receipt of colchicine. The primary endpoint was defined as in-hospital death within 28-days follow-up. Secondary endpoints included favorable change in the Ordinal Scale for Clinical Improvement on days 14 and 28 versus baseline, proportion of patients not requiring supplemental oxygen on days 14 and 28, and proportion of patients discharged by day 28. In total data for 303 PCR positive COVID-19 patients were extracted and 66 patients were included in the 1:1 matched cohort study. At the end of the 28 day follow-up, patients receiving colchicine were approximately five times more likely to be discharged (odds ratio, 5.0; 95% confidence interval, 1.25–20.1; p = 0.023) and when comparing mortality, there were 3 deaths (9.1%) in patients receiving colchicine versus 11 deaths (33.3%) in the groups receiving standard of care (odds ratio, 0.20; 95% confidence interval, 0.05–0.80; p = 0.023). These observations warrant further investigation in large controlled clinical trials.
Bone Morphogenetic Protein-2 in Development and Bone Homeostasis
Authors: Halloran Daniel , Durbano Hilary W. , Nohe Anja
Published: September 2020

Bone morphogenetic proteins (BMPs) are multi-functional growth factors belonging to the Transforming Growth Factor-Beta (TGF-β) superfamily. These proteins are essential to many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Specifically, within the BMP family, Bone Morphogenetic Protein-2 (BMP-2) was the first BMP to be characterized and has been well-studied. BMP-2 has important roles during embryonic development, as well as bone remodeling and homeostasis in adulthood. Some of its specific functions include digit formation and activating osteogenic genes, such as Runt-Related Transcription Factor 2 (RUNX2). Because of its diverse functions and osteogenic potential, the Food and Drug Administration (FDA) approved usage of recombinant human BMP-2 (rhBMP-2) during spinal fusion surgery, tibial shaft repair, and maxillary sinus reconstructive surgery. However, shortly after initial injections of rhBMP-2, several adverse complications were reported, and alternative therapeutics have been developed to limit these side-effects. As the clinical application of BMP-2 is largely implicated in bone, we focus primarily on its role in bone. However, we also describe briefly the role of BMP-2 in development. We then focus on the structure of BMP-2, its activation and regulation signaling pathways, BMP-2 clinical applications, and limitations of using BMP-2 as a therapeutic. Further, this review explores other potential treatments that may be useful in treating bone disorders.
Discovery of N-glycan Biomarkers for the Canine Osteoarthritis
Authors: Lee Hyunjun , Lee Ahyun , Seo Nari , Oh Jiwon , Kweon Oh-Kyeong , An Hyun Joo , Kim Jaehan
Published: September 2020

Protein glycosylation is a post-translational modification that impacts on protein activity, stability, and interactions. It was sensitively altered by the cellular state and, therefore, is now used for a diagnostic or prognostic indicator of various human diseases such as cancer. To evaluate the clinical feasibility in the veterinary area, the N-glycan biomarkers were discovered from canine serum for the diagnosis of osteoarthritis (OA), which is one of the most common diseases of dogs. N-glycome was obtained from 20 μL of canine serum by the enzymatic cleavage followed by the purification and enrichment using solid-phase extraction. Independent compositions of 163 and 463 N-glycans were found from healthy control (n = 41) and osteoarthritis patients (n = 92), respectively. Initially, 31 of the potential biomarkers were screened by the p-values below 1.0 × 10−10 from ANOVA. Then, the area under the curve (AUC) and the intensity ratio between OA patient and healthy control (P/C ratio) were calculated. Considering the diagnostic efficacy, the AUC bigger than 0.9 and the P/C ratio larger than 3.0 were used to discover 16 N-glycans as diagnostic biomarkers. Particularly, five of the diagnostic biomarkers were AUC above 0.99 and three of N-glycans had AUC 1.0. The results suggest a clear possibility for N-glycan biomarkers to be used as a clinical tool in the veterinary medical area enabling to provide objective and non-invasive diagnostic information.
Exercise Preconditioning Attenuates the Response to Experimental Colitis and Modifies Composition of Gut Microbiota in Wild-Type Mice
Authors: Cho Jinkyung , Kim Donghyun , Kang Hyunsik
Published: September 2020

This study investigated the suppressive effect of exercise preconditioning against colitis induced by high-fat diet (HF) plus dextran sulfate sodium (DSS) in wild-type mice. Male mice (C57BL/6) aged 6 weeks were assigned to standard chow (SC, n = 10) or HF (n = 10) or HF followed by DSS (HF+DSS, n = 10) or exercise preconditioning (EX) followed by HF+DSS (EX+HF+DSS, n = 10) for a total of 15 weeks. After 12 weeks of dietary treatments and/or exercise preconditioning, mice in the DSS groups were subjected to administration of 2 cycles of 5-day DSS (2% w/v) with a 7-day interval between cycles. HF resulted in colitis symptoms and histological changes, infiltration of immunity cells, decreased gut barrier proteins, increased pro-inflammatory and chemotactic cytokines and decreased anti-inflammatory cytokine such as adiponectin, which deteriorated after administration of DSS. Exercise preconditioning alleviated HF+DSS-induced colitis and caused significant modifications in gut microbiota: decreased Bacteroides vulgatus (p = 0.050) and increased Akkermansia muciniphila (p = 0.050). The current findings suggest that exercise preconditioning attenuates the severity of HF+DSS-induced colitis in C57BL/6 mice.
Novel Hybrid Biomass Anti-Aging Filler for Styrene-Butadiene Rubber Composites with Antioxidative and Reinforcing Properties
Authors: Guo Xiaohui , Luo Yuanfang , Chen Yongjun , Chen Lijuan , Jia Demin
Published: September 2020

Antioxidants are normally utilized to extend the service life of polymers due to the strong reducibility of the phenolic hydroxyl group of the hindered phenol structure. Inspired by this characteristic, we have introduced green tea polyphenol (TP) supported on a silica surface containing considerable phenolic hydroxyl groups to obtain a novel biomass anti-aging filler (BAF, denoted as silica-s-TP) to reinforce and improve the anti-aging property of rubber composites. The applying of silica-s-TP to enhance the thermal-oxidative stability and ultraviolet light (UV) aging resistance of styrene-butadiene rubber (SBR) was evaluated. The hybrid biomass anti-aging filler could not only uniformly disperse in the rubber matrix, giving rise to the excellent mechanical properties, but also enhance the properties of thermal-oxidative stability and UV aging resistance with the increasing silica-s-TP content of SBR distinctly. This study provides a mild and environmentally friendly strategy to prepare the functional biomass filler, which could be applied as not only a reinforcement filler but also an anti-aging additive in “green rubber”.
Long-Term Investigation into the Membrane Fouling Behavior in Anaerobic Membrane Bioreactors for Municipal Wastewater Treatment Operated at Two Different Temperatures
Authors: Ding Yi , Guo Zhansheng , Liang Zhenlin , Hou Xuguang , Li Zhipeng , Mu Dashuai , Ge Changzi , Zhang Chunpeng , Jin Chao
Published: September 2020

In this study, the characteristics of activated sludge flocs were investigated and their effects on the evolution of membrane fouling were considered in the anaerobic membrane bioreactors (AnMBR), which were operated at 25 and 35 °C for municipal wastewater treatment. It was found that the membrane fouling rate of the AnMBR at 25 °C was more severe than that at 35 °C. The membrane fouling trends were not consistent with the change in the concentration of soluble microbial product (SMP). The larger amount of SMP in the AnMBR at 35 °C did not induce more severe membrane fouling than that in the AnMBR at 25 °C. However, the polysaccharide and protein concentration of extracellular polymeric substance (EPS) was higher in the AnMBR at 25 °C in comparison with that in the AnMBR at 35 °C, and the protein/polysaccharide ratio of the EPS in the AnMBR at 25 °C was higher in contrast to that in the AnMBR at 35 °C. Meanwhile, the fouling tendencies measured for the AnMBRs could be related to the characteristics of loosely bound EPS and tightly bound EPS. The analysis of the activated sludge flocs characteristics indicated that a smaller sludge particle size and more fine flocs were observed at the AnMBR with 25 °C. Therefore, the membrane fouling potential in the AnMBR could be explained by the characteristics of activated sludge flocs.
The Synthesis and Characterization of Novel Bi-/Trimetallic Nanoparticles and Their Nanocomposite Membranes for Envisaged Water Treatment
Authors: Ndlwana Lwazi , Sikhwivhilu Keneiloe , Moutloali Richard Motlhaletsi , Ngila Jane Catherine
Published: September 2020

The impact of worldwide water scarcity, further exacerbated by environmental pollution, necessitates the development of effective water treatment membranes. Herein, we report the synthesis and characterization of nanocomposite membranes containing hyperbranched polyethyleneimine (HPEI) stabilized bi-and trimetallic nanoparticles. These membranes were prepared by blending a pre-grafted Polyethersulfone (PES) powder with the Pd@Fe@HPEI and Pd@FeAg@HPEI nanoparticles followed by phase inversion. The membranes, together with stabilized nanoparticles, were characterized by several analytical techniques, such as attenuated total reflectance–Fourier transform infra-red spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), X-ray diffractometry (XRD), optical contact angle (OCA), scanning electron microscopy (SEM), atomic force microscopy (AFM), and high-resolution transmission electron microscopy (HRTEM). These techniques revealed the elemental composition, zerovalent nature of the nanoparticles, and their small and even size distribution. Surface analysis showed chemical bonding between the polymeric functional groups and the supported nanoparticles. Furthermore, the nanocomposite membranes were found to be hydrophilic. Additionally, the membranes were investigated for swelling (water uptake), porosity, pore size, pure water permeation fluxes, and they indicated a decreased protein adhesion property. As such, the membranes fabricated in this work indicate the required properties for application in water treatment.
Beverage–Drug Interaction: Effects of Green Tea Beverage Consumption on Atorvastatin Metabolism and Membrane Transporters in the Small Intestine and Liver of Rats
Authors: Yao Hsien-Tsung , Hsu Ya-Ru , Li Mei-Ling
Published: September 2020

Green tea (GT) beverages are popular worldwide and may prevent the development of many chronic diseases including cardiovascular disease and cancer. To investigate whether the consumption of a GT beverage causes drug interactions, the effects of GT beverage consumption on atorvastatin metabolism and membrane transporters were evaluated. Male rats were fed a chow diet with tap water or the GT beverage for 3 weeks. Then, the rats were given a single oral dose (10 mg/kg body weight (BW)) of atorvastatin (ATV), and blood was collected at various time points within 6 h. The results show that GT consumption increased the plasma concentrations (AUC0–6h) of ATV (+85%) and 2-OH ATV (+93.3%). GT also increased the 2-OH ATV (+40.9%) and 4-OH ATV (+131.6%) contents in the liver. Decreased cytochrome P450 (CYP) 3A enzyme activity, with no change in P-glycoprotein expression in the intestine, was observed in rats treated with GT. Additionally, GT increased hepatic CYP3A-mediated ATV metabolism and decreased organic anion transporting polypeptides (OATP) 2 membrane protein expression. There was no significant difference in the membrane protein expression of OATP2B1 and P-glycoprotein in the intestine and liver after the GT treatment. The results show that GT consumption may lower hepatic OATP2 and, thus, limit hepatic drug uptake and increase plasma exposure to ATV and 2-OH ATV.
Effect of pH on Total Volume Membrane Charge Density in the Nanofiltration of Aqueous Solutions of Nitrate Salts of Heavy Metals
Authors: Marecka-Migacz Agata , Mitkowski Piotr Tomasz , Nędzarek Arkadiusz , Różański Jacek , Szaferski Waldemar
Published: September 2020

The separation efficiencies of aqueous solutions containing nitric salts of Zn, Cu, Fe or Pb at various pH in process of nanofiltration have been investigated experimentally. These results were used to obtain the total volume membrane charge densities, through mathematical modelling based on the Donnan–Steric partitioning Model. The experimentally obtained retention values of individual heavy metal ions varied between 36% (Zn2+ at pH = 2), 57% (Pb2+ at pH = 2), 80% (Fe3+ at pH = 9), and up to 97% (Cu2+ at pH = 9). The mathematical modelling allowed for fitting the total volume membrane charge density (Xd), which yielded values ranging from −451.90 to +900.16 mol/m3 for different non-symmetric ions. This study presents the application of nanofiltration (NF) modelling, including a consideration of each ion present in the NF system—even those originating from solutions used to adjust the pH values of the feed.
Welded Construction Design of Transition Fittings from Metal Pipes to Plastic Pipes
Authors: Dobrotă Dan , Rotaru Ionela , Bondrea Ioan
Published: September 2020

Transition type fittings are components often used in facilities where fluids are transported that allow the passage from a high density polyethylene (HDPE) pipe to a steel pipe. In the presented studies, four types of transition fittings were analyzed in the first stage. The four types of transition fittings are distinguished by the shape of their welded steel construction. The performed analyses took into account testing the behavior upon exposure to fatigue, measuring the HDPE hardness and applying the finite element method (FEM). As a result of these studies it was demonstrated that the form of the welded steel construction has a very great influence on the operating behavior of the transition fitting. Thus, a new transition fitting with a welded steel construction was designed. In this new type of transition fitting, an approximately 50% increase in resistance to fatigue stress, an approximately 90% reduction in stress in the part material and a reduction in the hardness of the material in HDPE pipes was obtained. The studies allow not only an improvement of the characteristics for these types of parts, but also the optimization of other types of steel-plastic joints.
Separation of the Formation Mechanisms of Residual Stresses in LPBF 316L
Authors: Ulbricht Alexander , Altenburg Simon J. , Sprengel Maximilian , Sommer Konstantin , Mohr Gunther , Fritsch Tobias , Mishurova Tatiana , Serrano-Munoz Itziar , Evans Alexander , Hofmann Michael , Bruno Giovanni
Published: September 2020

Rapid cooling rates and steep temperature gradients are characteristic of additively manufactured parts and important factors for the residual stress formation. This study examined the influence of heat accumulation on the distribution of residual stress in two prisms produced by Laser Powder Bed Fusion (LPBF) of austenitic stainless steel 316L. The layers of the prisms were exposed using two different border fill scan strategies: one scanned from the centre to the perimeter and the other from the perimeter to the centre. The goal was to reveal the effect of different heat inputs on samples featuring the same solidification shrinkage. Residual stress was characterised in one plane perpendicular to the building direction at the mid height using Neutron and Lab X-ray diffraction. Thermography data obtained during the build process were analysed in order to correlate the cooling rates and apparent surface temperatures with the residual stress results. Optical microscopy and micro computed tomography were used to correlate defect populations with the residual stress distribution. The two scanning strategies led to residual stress distributions that were typical for additively manufactured components: compressive stresses in the bulk and tensile stresses at the surface. However, due to the different heat accumulation, the maximum residual stress levels differed. We concluded that solidification shrinkage plays a major role in determining the shape of the residual stress distribution, while the temperature gradient mechanism appears to determine the magnitude of peak residual stresses.
Immune Organs and Immune Cells on a Chip: An Overview of Biomedical Applications
Authors: Morsink Margaretha , Willemen Niels , Leijten Jeroen , Bansal Ruchi , Shin Su
Published: September 2020

Understanding the immune system is of great importance for the development of drugs and the design of medical implants. Traditionally, two-dimensional static cultures have been used to investigate the immune system in vitro, while animal models have been used to study the immune system’s function and behavior in vivo. However, these conventional models do not fully emulate the complexity of the human immune system or the human in vivo microenvironment. Consequently, many promising preclinical findings have not been reproduced in human clinical trials. Organ-on-a-chip platforms can provide a solution to bridge this gap by offering human micro-(patho)physiological systems in which the immune system can be studied. This review provides an overview of the existing immune-organs-on-a-chip platforms, with a special emphasis on interorgan communication. In addition, future challenges to develop a comprehensive immune system-on-chip model are discussed.