bluuebunny/crossref_metadata_2025 huggingface dataset:
Gene Expression Analysis for Uterine Cervix and Corpus Cancer Characterization
Authors: Almorox Lucía , Antequera Laura , Rojas Ignacio , Herrera Luis Javier , Ortuño Francisco M.
Published: February 2024

The analysis of gene expression quantification data is a powerful and widely used approach in cancer research. This work provides new insights into the transcriptomic changes that occur in healthy uterine tissue compared to those in cancerous tissues and explores the differences associated with uterine cancer localizations and histological subtypes. To achieve this, RNA-Seq data from the TCGA database were preprocessed and analyzed using the KnowSeq package. Firstly, a kNN model was applied to classify uterine cervix cancer, uterine corpus cancer, and healthy uterine samples. Through variable selection, a three-gene signature was identified (VWCE, CLDN15, ADCYAP1R1), achieving consistent 100% test accuracy across 20 repetitions of a 5-fold cross-validation. A supplementary similar analysis using miRNA-Seq data from the same samples identified an optimal two-gene miRNA-coding signature potentially regulating the three-gene signature previously mentioned, which attained optimal classification performance with an 82% F1-macro score. Subsequently, a kNN model was implemented for the classification of cervical cancer samples into their two main histological subtypes (adenocarcinoma and squamous cell carcinoma). A uni-gene signature (ICA1L) was identified, achieving 100% test accuracy through 20 repetitions of a 5-fold cross-validation and externally validated through the CGCI program. Finally, an examination of six cervical adenosquamous carcinoma (mixed) samples revealed a pattern where the gene expression value in the mixed class aligned closer to the histological subtype with lower expression, prompting a reconsideration of the diagnosis for these mixed samples. In summary, this study provides valuable insights into the molecular mechanisms of uterine cervix and corpus cancers. The newly identified gene signatures demonstrate robust predictive capabilities, guiding future research in cancer diagnosis and treatment methodologies.
Runs of Homozygosity Detection and Selection Signature Analysis for Local Goat Breeds in Yunnan, China
Authors: Huang Chang , Zhao Qian , Chen Qian , Su Yinxiao , Ma Yuehui , Ye Shaohui , Zhao Qianjun
Published: February 2024

Runs of Homozygosity (ROH) are continuous homozygous DNA segments in diploid genomes, which have been used to estimate the genetic diversity, inbreeding levels, and genes associated with specific traits in livestock. In this study, we analyzed the resequencing data from 10 local goat breeds in Yunnan province of China and five additional goat populations obtained from a public database. The ROH analysis revealed 21,029 ROH segments across the 15 populations, with an average length of 1.27 Mb, a pattern of ROH, and the assessment of the inbreeding coefficient indicating genetic diversity and varying levels of inbreeding. iHS (integrated haplotype score) was used to analyze high-frequency Single-Nucleotide Polymorphisms (SNPs) in ROH regions, specific genes related to economic traits such as coat color and weight variation. These candidate genes include OCA2 (OCA2 melanosomal transmembrane protein) and MLPH (melanophilin) associated with coat color, EPHA6 (EPH receptor A6) involved in litter size, CDKAL1 (CDK5 regulatory subunit associated protein 1 like 1) and POMC (proopiomelanocortin) linked to weight variation and some putative genes associated with high-altitude adaptability and immune. This study uncovers genetic diversity and inbreeding levels within local goat breeds in Yunnan province, China. The identification of specific genes associated with economic traits and adaptability provides actionable insights for utilization and conservation efforts.
Multiplex Real-Time PCR-Based Newborn Screening for Severe Primary Immunodeficiency and Spinal Muscular Atrophy in Osaka, Japan: Our Results after 3 Years
Authors: Kimizu Tomokazu , Nozaki Masatoshi , Okada Yousuke , Sawada Akihisa , Morisaki Misaki , Fujita Hiroshi , Irie Akemi , Matsuda Keiko , Hasegawa Yuiko , Nishi Eriko , Okamoto Nobuhiko , Kawai Masanobu , Imai Kohsuke , Suzuki Yasuhiro , Wada Kazuko , Mitsuda Nobuaki , Ida Shinobu
Published: February 2024

In newborn screening (NBS), it is important to consider the availability of multiplex assays or other tests that can be integrated into existing systems when attempting to implement NBS for new target diseases. Recent developments in innovative testing technology have made it possible to simultaneously screen for severe primary immunodeficiency (PID) and spinal muscular atrophy (SMA) using quantitative real-time polymerase chain reaction (qPCR) assays. We describe our experience of optional NBS for severe PID and SMA in Osaka, Japan. A multiplex TaqMan qPCR assay was used for the optional NBS program. The assay was able to quantify the levels of T-cell receptor excision circles and kappa-deleting recombination excision circles, which is useful for severe combined immunodeficiency and B-cell deficiency screening, and can simultaneously detect the homozygous deletion of SMN1 exon 7, which is useful for NBS for SMA. In total, 105,419 newborns were eligible for the optional NBS program between 1 August 2020 and 31 August 2023. A case each of X-linked agammaglobulinemia and SMA were diagnosed through the optional NBS and treated at early stages (before symptoms appeared). Our results show how multiplex PCR-based NBS can benefit large-scale NBS implementation projects for new target diseases.
Cloning and Analysis of Expression of Genes Related to Carotenoid Metabolism in Different Fruit Color Mutants of Pepper (Capsicum annuum L.)
Authors: Feng Penglong , Wang Yayi , Wen Junqin , Ren Yanjing , Zhong Qiwen , Li Quanhui
Published: February 2024

The formation of fruit color in pepper is closely related to the processes of carotenoid metabolism. In this study, red wild-type pepper XHB, SP01, PC01 and their corresponding mutants H0809 (orange), SP02 (yellow), and PC02 (orange) were used as research materials. The Ggps, Psy, Lcyb, Crtz, Zep, and Ccs genes involved in carotenoid biosynthesis were cloned, and bioinformatics and expression analyses were carried out. The results showed that the full lengths of the six genes were 1110 bp, 2844 bp, 1497 bp, 2025 bp, 510 bp, and 1497 bp, and they encoded 369, 419, 498, 315, 169, and 498 amino acids, respectively. Except for the full-length Ccs gene, which could not be amplified in the yellow mutant SP02 and the orange mutant PC02, the complete full-length sequences of the other genes could be amplified in different materials, indicating that the formation of fruit color in the SP02 and PC02 mutants could be closely related to the deletion or mutation of the Ccs gene. The analytical results of real-time quantitative reverse transcription PCR (qRT-PCR) showed that the Ggps, Psy, Lcyb, Crtz, and Zep genes were expressed at different developmental stages of three pairs of mature-fruit-colored materials, but their patterns of expression were not consistent. The orange mutant H0809 could be amplified to the full Ccs gene sequence, but its expression was maintained at a lower level. It showed a significant difference in expression compared with the wild-type XHB, indicating that the formation of orange mutant H0809 fruit color could be closely related to the different regulatory pattern of Ccs expression. The results provide a theoretical basis for in-depth understanding of the molecular regulatory mechanism of the formation of color in pepper fruit.
Massive Parallel DNA Sequencing of Patients with Inherited Cardiomyopathies in Cyprus and Suggestion of Digenic or Oligogenic Inheritance
Authors: Koutsofti Constantina , Ioannides Marios , Polydorou Christiana , Papagregoriou Gregory , Malatras Apostolos , Michael George , Hadjiioannou Irene , Pieri Stylianos , Loizidou Eleni M. , Eftychiou Christos , Papasavvas Elias , Christophides Theodoros , Alkelai Anna , Kapoor Manav , Shuldiner Alan R. , Avraamides Panayiotis , Deltas Constantinos
Published: February 2024

Inherited cardiomyopathies represent a highly heterogeneous group of cardiac diseases. DNA variants in genes expressed in cardiomyocytes cause a diverse spectrum of cardiomyopathies, ultimately leading to heart failure, arrythmias, and sudden cardiac death. We applied massive parallel DNA sequencing using a 72-gene panel for studying inherited cardiomyopathies. We report on variants in 25 families, where pathogenicity was predicted by different computational approaches, databases, and an in-house filtering analysis. All variants were validated using Sanger sequencing. Familial segregation was tested when possible. We identified 41 different variants in 26 genes. Analytically, we identified fifteen variants previously reported in the Human Gene Mutation Database: twelve mentioned as disease-causing mutations (DM) and three as probable disease-causing mutations (DM?). Additionally, we identified 26 novel variants. We classified the forty-one variants as follows: twenty-eight (68.3%) as variants of uncertain significance, eight (19.5%) as likely pathogenic, and five (12.2%) as pathogenic. We genetically characterized families with a cardiac phenotype. The genetic heterogeneity and the multiplicity of candidate variants are making a definite molecular diagnosis challenging, especially when there is a suspicion of incomplete penetrance or digenic-oligogenic inheritance. This is the first systematic study of inherited cardiac conditions in Cyprus, enabling us to develop a genetic baseline and precision cardiology.
The Devil Firefish Pterois miles (Bennett, 1828): Life History Traits of a Potential Fishing Resource in Rhodes (Eastern Mediterranean)
Authors: Kondylatos Gerasimos , Theocharis Alexandros , Mandalakis Manolis , Avgoustinaki Maria , Karagyaurova Teodora , Koulocheri Zoi , Vardali Sofia , Klaoudatos Dimitris
Published: February 2024

The devil firefish Pterois miles (Bennett 1828) is recognized as one of the 100 worst invasive alien species (IAS) in the Mediterranean Sea with significant socioeconomic and ecological implications. A total of 363 individuals were collected between April 2021 and March 2022 from the Levantine Sea (the Eastern Mediterranean), aiming to assess the current progression of the population. The male to female ratio (1:1.03) did not significantly depart from 1:1, with length–weight relationships exhibiting positive allometric growth. Five age groups were identified, with the third and fourth age groups being the predominant (74% of the population). The highest reproductive intensity was observed during summer followed by autumn. The onset of sexual maturity (L50) for the total population was estimated at 22.44 cm in total length (2.1 years). The asymptotic length was estimated at 45.35 cm for the total population, with males growing larger than females. Longevity was estimated at 14.4 years for the total population. The inflection point was estimated at 4.31 years for the total population. Length with a 50% probability of capture (LC50) was estimated at 23.03 cm, with the respective age (t50) of 2.2 years. The exploitation rate (E = 0.48) indicated that the population is underexploited. The optimum (Fopt) and target (Flim) fishing mortality were higher in comparison with the present fishing mortality (F = 0.41), indicating a potential for commercial exploitation of the species.
‘They Talk about the Weather, but No One Does Anything about It’: A Mixed-Methods Study of Everyday Climate Change Conversations
Authors: Latkin Carl A. , Dayton Lauren , Winiker Abigail , Countess Kennedy , Hendrickson Zoé Mistrale
Published: February 2024

Understanding everyday conversations about climate change may provide insights into framing the issue to promote climate change action. As part of a longitudinal online study in the US launched in June 2021, 805 respondents were asked if they had discussed climate change with a friend or family member in the prior month; if not, why not, and if yes, they were asked to delineate the conversation topic. Concurrent mixed methods were used to analyze the data. The majority (62.6%) of respondents reported not having a conversation about climate change in the prior month. Among those who indicated that they had discussed climate change, five themes were identified from the conversation topics, with many having reported discussing the impact of climate change on weather patterns. Very few discussed actions to address climate change, and most of these discussions focused on individual-level behaviors rather than collective actions. Among participants who had not recently discussed climate change, the most prevalent theme was that it was not a priority or an issue they cared about. Results suggest that conversations may not lead to collective actions and that policymakers and environmental organizations should provide guidance on effectively channeling climate change concerns into action.
Adopting a WHO Framework Convention on Tobacco Control-Based Tobacco Control Law in Ethiopia: Sustained Transnational Health Advocacy and Multi-Sectoral Institutionalized Support
Authors: Crosbie Eric , Edison Kyle , Maclean Vandyke , Moges Dereje , Fuss Caroline , Muggli Monique E. , Bityeki Bintou Camara , Sebrié Ernesto M.
Published: February 2024

Objective: The objective of this study was to document how Ethiopia adopted a WHO Framework Convention on Tobacco Control (FCTC)-based tobacco control law. Methods: We analyzed publicly available documents, including news media articles, advocacy reports, and government documents. We triangulated these findings by interviewing nine key stakeholders. Data were analyzed to construct a historical and thematic narrative and analyzed through a retrospective policy analysis. Results: Local and international health advocacy efforts helped introduce and support WHO FCTC-based legislation by (1) educating policymakers about the WHO FCTC, (2) providing legal assistance in drafting legislation, (3) generating local data to counter industry claims, and (4) producing media advocacy to expose industry activity. Health advocates worked closely with government officials to create a multi-sectoral tobacco committee to institutionalize efforts and insulate tobacco companies from the policymaking process. Japan Tobacco International bought majority shares of the government-owned tobacco company and attempted to participate in the process, using standard industry tactics to undermine legislative efforts. However, with health advocacy assistance, government officials were able to reject these attempts and adopt a WHO FCTC-based law in 2019 that included 100% smoke-free indoor places, a comprehensive ban on tobacco advertising, and large pictorial health warning labels, among other provisions. Conclusion: Sustained local health advocacy efforts supported by international technical and financial assistance can help establish WHO FCTC-based tobacco control laws. Applying a standardized multi-sectoral approach can establish coordinating mechanisms to further institutionalize the WHO FCTC as a legal tool to build support with other government sectors and insulate the tobacco industry from the policymaking process.
Protein Kinase D Plays a Crucial Role in Maintaining Cardiac Homeostasis by Regulating Post-Translational Modifications of Myofilament Proteins
Authors: Herwig Melissa , Begovic Merima , Budde Heidi , Delalat Simin , Zhazykbayeva Saltanat , Sieme Marcel , Schneider Luca , Jaquet Kornelia , Mügge Andreas , Akin Ibrahim , El-Battrawy Ibrahim , Fielitz Jens , Hamdani Nazha
Published: February 2024

Protein kinase D (PKD) enzymes play important roles in regulating myocardial contraction, hypertrophy, and remodeling. One of the proteins phosphorylated by PKD is titin, which is involved in myofilament function. In this study, we aimed to investigate the role of PKD in cardiomyocyte function under conditions of oxidative stress. To do this, we used mice with a cardiomyocyte-specific knock-out of Prkd1, which encodes PKD1 (Prkd1loxP/loxP; αMHC-Cre; PKD1 cKO), as well as wild type littermate controls (Prkd1loxP/loxP; WT). We isolated permeabilized cardiomyocytes from PKD1 cKO mice and found that they exhibited increased passive stiffness (Fpassive), which was associated with increased oxidation of titin, but showed no change in titin ubiquitination. Additionally, the PKD1 cKO mice showed increased myofilament calcium (Ca2+) sensitivity (pCa50) and reduced maximum Ca2+-activated tension. These changes were accompanied by increased oxidation and reduced phosphorylation of the small myofilament protein cardiac myosin binding protein C (cMyBPC), as well as altered phosphorylation levels at different phosphosites in troponin I (TnI). The increased Fpassive and pCa50, and the reduced maximum Ca2+-activated tension were reversed when we treated the isolated permeabilized cardiomyocytes with reduced glutathione (GSH). This indicated that myofilament protein oxidation contributes to cardiomyocyte dysfunction. Furthermore, the PKD1 cKO mice exhibited increased oxidative stress and increased expression of pro-inflammatory markers interleukin (IL)-6, IL-18, and tumor necrosis factor alpha (TNF-α). Both oxidative stress and inflammation contributed to an increase in microtubule-associated protein 1 light chain 3 (LC3)-II levels and heat shock response by inhibiting the mammalian target of rapamycin (mTOR) in the PKD1 cKO mouse myocytes. These findings revealed a previously unknown role for PKD1 in regulating diastolic passive properties, myofilament Ca2+ sensitivity, and maximum Ca2+-activated tension under conditions of oxidative stress. Finally, we emphasized the importance of PKD1 in maintaining the balance of oxidative stress and inflammation in the context of autophagy, as well as cardiomyocyte function.
Genomic and Epigenomic Changes in the Progeny of Cold-Stressed Arabidopsis thaliana Plants
Authors: Rahman Ashif , Yadav Narendra Singh , Byeon Boseon , Ilnytskyy Yaroslav , Kovalchuk Igor
Published: February 2024

Plants are continuously exposed to various environmental stresses. Because they can not escape stress, they have to develop mechanisms of remembering stress exposures somatically and passing it to the progeny. We studied the Arabidopsis thaliana ecotype Columbia plants exposed to cold stress for 25 continuous generations. Our study revealed that multigenerational exposure to cold stress resulted in the changes in the genome and epigenome (DNA methylation) across generations. Main changes in the progeny were due to the high frequency of genetic mutations rather than epigenetic changes; the difference was primarily in single nucleotide substitutions and deletions. The progeny of cold-stressed plants exhibited the higher rate of missense non-synonymous mutations as compared to the progeny of control plants. At the same time, epigenetic changes were more common in the CHG (C = cytosine, H = cytosine, adenine or thymine, G = guanine) and CHH contexts and favored hypomethylation. There was an increase in the frequency of C to T (thymine) transitions at the CHH positions in the progeny of cold stressed plants; because this type of mutations is often due to the deamination of the methylated cytosines, it can be hypothesized that environment-induced changes in methylation contribute to mutagenesis and may be to microevolution processes and that RNA-dependent DNA methylation plays a crucial role. Our work supports the existence of heritable stress response in plants and demonstrates that genetic changes prevail.
The Role of Transglutaminase 2 in Cancer: An Update
Authors: Zaltron Elisabetta , Vianello Federica , Ruzza Alessia , Palazzo Alberta , Brillo Valentina , Celotti Ilaria , Scavezzon Matteo , Rossin Federica , Leanza Luigi , Severin Filippo
Published: February 2024

Transglutaminase type 2 (TG2) is the most ubiquitously expressed and well characterized member of the transglutaminase family. It is a ubiquitous multifunctional enzyme implicated in the regulation of several cellular pathways that support the survival, death, and general homeostasis of eukaryotic cells. Due to its multiple localizations both inside and outside the cell, TG2 participates in the regulation of many crucial intracellular signaling cascades in a tissue- and cell-specific manner, making this enzyme an important player in disease development and progression. Moreover, TG2 is capable of modulating the tumor microenvironment, a process of dynamic tissue remodeling and biomechanical events, resulting in changes which influence tumor initiation, growth, and metastasis. Even if generally related to the Ca2+-dependent post-translational modification of proteins, a number of different biological functions have been ascribed to TG2, like those of a peptide isomerase, protein kinase, guanine nucleotide binder, and cytosolic–nuclear translocator. With respect to cancer, TG2′s role is controversial and highly debated; it has been described both as an anti- and pro-apoptotic factor and is linked to all the processes of tumorigenesis. However, numerous pieces of evidence support a tissue-specific role of TG2 so that it can assume both oncogenic and tumor-suppressive roles.
Optimization of Traditional Stock Market Strategies Using the LSTM Hybrid Approach
Authors: Botunac Ive , Bosna Jurica , Matetić Maja
Published: February 2024

Investment decision-makers increasingly rely on modern digital technologies to enhance their strategies in today’s rapidly changing and complex market environment. This paper examines the impact of incorporating Long Short-term Memory (LSTM) models into traditional trading strategies. The core investigation revolves around whether strategies enhanced with LSTM technology perform better than traditional methods alone. Traditional trading strategies typically depend on analyzing current closing prices and various technical indicators to take trading action. However, by applying LSTM models, this study aims to forecast closing prices with greater accuracy, thereby improving trading performance. Our findings indicate that trading strategies that utilize LSTM models outperform traditional strategies. This improvement suggests a significant advantage in using LSTM models for market prediction and trading decision making. Acknowledging that no one-size-fits-all strategy works for every market condition or stock is crucial. As such, traders are encouraged to select and tailor their strategies based on thorough testing and analysis to best suit their needs and market conditions. This study contributes to a better understanding of how integrating LSTM models can enhance traditional trading strategies, offering a path toward more effective decision making in the unpredictable stock market.
A Meta-Analysis of the Global Prevalence of Temporomandibular Disorders
Authors: Zieliński Grzegorz , Pająk-Zielińska Beata , Ginszt Michał
Published: February 2024

Background: This meta-analysis aims to evaluate the proportion of people with TMDs in different studies, considering factors such as geographical region, patient age, and sample size. Methods: The search yielded 6984 articles on the incidence of TMDs. Finally, 74 studies with 172,239 subjects and 35,259 with TMDs were selected for final analysis. Analyses were performed using the R statistical language. Results: The incidence of TMDs in the world population was 34%. The age group 18–60 years is the most exposed to TMDs. From the data presented, we observed that for each continent, the female group was 9% to 56% larger than the male group. The highest female-to-male ratio (F:M) was reported in South America (1.56), whereas the lowest F:M ratio was reported in Europe (1.09), suggesting an almost equal distribution of males and females. Conclusions: This suggests that geographical location may play a role in the results of the studies. The prevalence of TMDs was significantly higher in South America (47%) compared to Asia (33%) and Europe (29%). Larger epidemiological studies of TMDs in African and Australian populations are recommended. In conclusion, both visual and statistical assessments suggest that the results of our meta-analysis are robust and unlikely to be significantly affected by publication bias. This suggests that geographical location may play a role in the prevalence of TMDs.
All-Suture Anchor vs. Knotless Suture Anchor for the Treatment of Anterior Shoulder Instability—A Prospective Cohort Study
Authors: Minkus Marvin , Aigner Annette , Wolke Julia , Scheibel Markus
Published: February 2024

All-suture or soft-anchors (SA) represent a new generation of suture anchor technology with a completely suture-based system. This study’s objective was to assess Juggerknot® SA, for arthroscopic Bankart repair in recurrent shoulder instability (RSI), and to compare it to a commonly performed knotless anchor (KA) technique (Pushlock®). In a prospective cohort study, 30 consecutive patients scheduled for reconstruction of the capsulolabral complex without substantial glenoid bone loss were included and operated on using the SA technique. A historical control group was operated on using the KA technique for the same indication. Clinical examinations were performed preoperatively and 12 and 24 months postoperatively. RSI and WOSI at 24 months were the co-primary endpoints, evaluated with logistic and linear regression. A total of 5 out of 30 (16.7%) patients suffered from RSI in the SA group, one out of 31 (3.2%) in the KA group (adjusted odds ratio = 10.12, 95% CI: 0.89–115.35), and 13.3% in the SA group and 3.2% in the KAgroup had a revision. The median WOSI in the SA group was lower than in the KA group (81% vs. 95%) (adjusted regression coefficient = 10.12, 95% CI: 0.89–115.35). Arthroscopic capsulolabral repair for RSI using either the SA or KA technique led to satisfying clinical outcomes. However, there is a tendency for higher RSI and lower WOSI following the SA technique.
Evaluation of Choroidal Structure in Type 1 Macular Neovascularization Using Different Optical Coherence Tomography Analyses: Scale Bar and Binarization
Authors: Hirai Hiromasa , Yamashita Mariko , Ijuin Nobuo , Jimura Hironobu , Nishi Tomo , Ogata Nahoko , Ueda Tetsuo
Published: February 2024

Background: Macular neovascularization (MNV) has been evaluated by optical coherence tomography (OCT) imaging using various approaches. However, few studies have examined their differences. This study analyzed type 1 MNV with a combination of two approaches: scale bar and binarization. Methods: We enrolled 84 patients with untreated type 1 MNV. We measured choroidal parameters using a scale bar and defined the ratios of superficial choroidal thickness to choroidal vessel diameter (SV ratios). We also used binarization and calculated the ratios of the luminal to the choroidal area (LC ratios) in two directions (horizontal and vertical). Results: Fifty-one patients (61%) were classified as having polyps. SV ratios in the group with polyps were significantly lower than in the group without (p < 0.001). The receiver operating characteristic (ROC) curve showed that the SV ratio was predictive of polyps (AUC 0.733, 95% CI: 0.621–0.844). In patients without polyps, horizontal LC ratios were significantly higher in a subgroup with subretinal fluid than in those without (p = 0.047). The ROC curve showed that the LC ratio was predictive of subretinal fluid (AUC 0.722, 95% CI: 0.517–0.926). Conclusion: The SV ratio reflects the MNV disease type, whereas the LC ratio reflects MNV disease activity. Establishing cut-off values for each ratio may be useful for MNV diagnosis.
Privacy-Preserving Face Recognition Method Based on Randomization and Local Feature Learning
Authors: Huang Yanhua , Wu Zhendong , Chen Juan , Xiang Hui
Published: February 2024

Personal privacy protection has been extensively investigated. The privacy protection of face recognition applications combines face privacy protection with face recognition. Traditional face privacy-protection methods encrypt or perturb facial images for protection. However, the original facial images or parameters need to be restored during recognition. In this paper, it is found that faces can still be recognized correctly when only some of the high-order and local feature information from faces is retained, while the rest of the information is fuzzed. Based on this, a privacy-preserving face recognition method combining random convolution and self-learning batch normalization is proposed. This method generates a privacy-preserved scrambled facial image and an image fuzzy degree that is close to an encryption of the image. The server directly recognizes the scrambled facial image, and the recognition accuracy is equivalent to that of the normal facial image. The method ensures the revocability and irreversibility of the privacy preserving of faces at the same time. In this experiment, the proposed method is tested on the LFW, Celeba, and self-collected face datasets. On the three datasets, the proposed method outperforms the existing face privacy-preserving recognition methods in terms of face visual information elimination and recognition accuracy. The recognition accuracy is >99%, and the visual information elimination is close to an encryption effect.
Fatigue Life and Residual Stress of Flat Stainless Steel Specimens Laser-Cladded with a Cobalt-Based Alloy and Postprocessed with Laser Shock Peening
Authors: Flores-García Santiago , Martínez-Pérez Carlos E. , Rubio-González Carlos , Banderas-Hernández J. Antonio , Félix-Martínez Christian , Jiménez Salomón M. A.
Published: February 2024

Laser cladding (LC) is a versatile additive manufacturing process where strands of metallic material are deposited and melted by a laser. However, there are some limitations associated with this process that may affect the performance of the final manufactured parts. In the present work, the influence of laser shock peening (LSP) on the fatigue life of 304 stainless steel flat specimens with a cobalt-based alloy (Stellite 6) coating applied by LC was investigated. The analysis was carried out both experimentally and numerically. In the LSP simulation, the ABAQUS/Explicit code was used to determine the residual stress distribution of specimens with double central notches with a radius of curvature of 5, 10, 15, and 20 mm. From the numerical results, an improvement was found regarding fatigue life up to 48% in samples with LSP. Experimentally, 14% in fatigue life enhancement was observed. The residual stress, determined by the contour method, showed good agreement with the LSP simulation. The SEM images revealed that the fatigue failure started at the Stellite 6 coating and propagated towards the center of the specimen. LSP has been shown to be a suitable postprocessing alternative for laser-cladded parts that will be subjected to fatigue loading since it led to fatigue improvement through the introduction of compressive residual stresses on clad coatings.
5-Fluorouracil/Coumarin and 5-Fluorouracil/Chromone Hybrids: Synthesis and Drug-Likeness Modeling
Authors: Giraldo-Arroyave Laura , Yepes Andrés F. , Cardona-Galeano Wilson
Published: February 2024

A series of 5-fluorouracil/coumarin and 5-fluorouracil/chromone hybrids were synthesized with good yields using click chemistry as the key step. The structures of these compounds and all intermediates were elucidated by spectroscopic analysis. Furthermore, pharmacokinetic and drug-like computations taken together indicated that the novel hybrids have a strong possibility to advance to further biological studies.
1,4-Bis(2-((1R,5S)-6,6-dimethylbicyclo[3.1.1]hept-2-en-2-yl)ethyl)piperazine
Authors: Li-Zhulanov Nikolai S. , Rogachev Artem D. , Gatilov Yuri V. , Volcho Konstantin P. , Salakhutdinov Nariman F.
Published: February 2024

The reaction of (−)-nopol mesylate with piperazine in acetonitrile under reflux, afforded symmetric 1,4-bis(2-((1R,5S)-6,6-dimethylbicyclo[3.1.1]hept-2-en-2-yl)ethyl)piperazine in a good yield. The compound was fully characterized and its structure was confirmed using X-ray diffraction analysis.
Inhibition Effect of Triphenylmethane Dyes for the Corrosion of Carbon Steel in CO2-Saturated NaCl Corrosion Medium
Authors: Peng Lincai , Wen Shaomu , Yan Jing , Yu Huali , Wen Zhan , Wang Zhi
Published: February 2024

Carbon dioxide corrosion presents a significant challenge in the oil and gas field. This study simulates the corrosive environment characteristics of oil and gas fields to investigate the corrosion inhibition properties of three triphenylmethane dyes. The inhibitive performance and mechanisms of these dyes were analyzed through weight loss and electrochemical testing, revealing that crystal violet (CV) exhibited a superior inhibition effectiveness over malachite green (MG) and Fuchsine basic (FB). At a concentration of 150 ppm in a CO2-saturated 5% NaCl solution at 25 °C, CV achieved an impressive maximum inhibition efficiency of 94.89%. With the increase in temperature, the corrosion rate slightly decreased, and the corrosion rate was 92.94% at 60 °C. The investigated CV acted as a mixed-type corrosion inhibitor and its protection obeyed the Langmuir adsorption isotherm. The corrosion morphology was characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and confocal laser scanning microscopy (CLMS). Quantum chemical calculations and molecular dynamics simulations were employed to validate the corrosion inhibition mechanisms, providing guidance for the further application of these dyes in corrosion control.
Exploring the Potential of Crassostrea nippona Hydrolysates as Dietary Supplements for Mitigating Dexamethasone-Induced Muscle Atrophy in C2C12 Cells
Authors: Kurera M. J. M. S. , Nagahawatta D. P. , Liyanage N. M. , Jayawardhana H. H. A. C. K. , Dissanayake D. S. , Lee Hyo-Geun , Kim Young-Sang , Kang Sang In , Jeon You-Jin
Published: February 2024

Muscle atrophy is a detrimental and injurious condition that leads to reduced skeletal muscle mass and disruption of protein metabolism. Oyster (Crassostrea nippona) is a famous and commonly consumed shellfish in East Asia and has become a popular dietary choice worldwide. The current investigation evaluated the efficacy of C. nippona against muscle atrophy, which has become a severe health issue. Mammalian skeletal muscles are primarily responsible for efficient metabolism, energy consumption, and body movements. The proteins that regulate muscle hypertrophy and atrophy are involved in muscle growth. C. nippona extracts were enzymatically hydrolyzed using alcalase (AOH), flavourzyme (FOH), and protamex (POH) to evaluate their efficacy in mitigating dexamethasone-induced muscle damage in C2C12 cells in vitro. AOH exhibited notable cell proliferative abilities, promoting dose-dependent myotube formation. These results were further solidified by protein expression analysis. Western blot and gene expression analysis via RT-qPCR demonstrated that AOH downregulated MuRF-1, Atrogin, Smad 2/3, and Foxo-3a, while upregulating myogenin, MyoD, myosin heavy chain expression, and mTOR, key components of the ubiquitin–proteasome and mTOR signaling pathways. Finally, this study suggests that AOH holds promise for alleviating dexamethasone-induced muscle atrophy in C2C12 cells in vitro, offering insights for developing functional foods targeting conditions akin to sarcopenia.
Exploring the Interplay between Complex Post-Traumatic Stress Disorder and Obsessive–Compulsive Disorder Severity: Implications for Clinical Practice
Authors: D’Angelo Martina , Valenza Marta , Iazzolino Anna Maria , Longobardi Grazia , Di Stefano Valeria , Visalli Giulia , Steardo Luca , Scuderi Caterina , Manchia Mirko , Steardo Luca
Published: February 2024

Background and Objectives: Traumatic events adversely affect the clinical course of obsessive–compulsive disorder (OCD). Our study explores the correlation between prolonged interpersonal trauma and the severity of symptoms related to OCD and anxiety disorders. Materials and Methods: The study follows a cross-sectional and observational design, employing the International Trauma Questionnaire (ITQ) to examine areas linked to interpersonal trauma, the Hamilton Anxiety Rating Scale (HAM-A), and the Yale–Brown Obsessive–Compulsive Scale (Y-BOCS) to assess anxious and obsessive–compulsive symptoms, respectively. Descriptive analysis, analysis of variance (ANOVA), and logistic regression analyses were conducted. Results: We recruited 107 OCD-diagnosed patients, categorizing them into subgroups based on the presence or absence of complex post-traumatic stress disorder (cPTSD). The ANOVA revealed statistically significant differences between the two groups in the onset age of OCD (p = 0.083), psychiatric familial history (p = 0.023), HAM-A, and Y-BOCS (p < 0.0001). Logistic regression indicated a statistically significant association between the presence of cPTSD and Y-BOCS scores (p < 0.0001). Conclusions: The coexistence of cPTSD in OCD exacerbates obsessive–compulsive symptoms and increases the burden of anxiety. Further advancements in this field are crucial for mitigating the impact of early trauma on the trajectory of OCD and associated anxious symptoms.
Advancing Wire Arc Directed Energy Deposition: Analyzing Impact of Materials and Parameters on Bead Shape
Authors: Price Stephen , Judd Kiran , Gleason Matthew , Tsaknopoulos Kyle , Cote Danielle L. , Neamtu Rodica
Published: February 2024

This study advances foundational knowledge regarding the impact of processing parameters and material selection on bead shape in Wire Arc directed energy deposition (Wire Arc DED) additive manufacturing. Through the collection and analysis of the largest Wire Arc DED bead shape dataset to date, this work confirms the dominant roles of the feed rate and travel speed on bead shape. Specifically, an increasing feed rate correlates with an increased bead size, while increasing the travel speed decreases the bead size. Furthermore, as the first dataset to directly compare bead shape across different wire–substrate combinations, this research identified that material selection has a smaller, but still relevant, impact on bead shape compared to the feed rate and travel speed. These insights into the roles of parameters and materials are critical for improving large-scale manufacturing efficiency and quality with Wire Arc DED. By generating a robust, multi-material dataset, this work enables applications of machine learning to optimize Wire Arc DED through quicker manufacturing, reduced material waste, and improved structural integrity.
Advances in Carbon Dot-Based Ratiometric Fluorescent Probes for Environmental Contaminant Detection: A Review
Authors: Xing Xinxin , Wang Zhezhe , Wang Yude
Published: February 2024

Detecting environmental contaminants is crucial for protecting ecosystems and human health. While traditional carbon dot (CD) fluorescent probes are versatile, they may suffer from limitations like fluctuations in signal intensity, leading to detection inaccuracies. In contrast, ratiometric fluorescent probes, designed with internal self-calibration mechanisms, offer enhanced sensitivity and reliability. This review focuses on the design and applications of ratiometric fluorescent probes based on CDs for environmental monitoring. Our discussion covers construction strategies, ratiometric fluorescence principles, and applications in detecting various environmental contaminants, including organic pollutants, heavy metal ions, and other substances. We also explore associated advantages and challenges and provide insights into potential solutions and future research directions.
Modified Broadband Ruthroff-Type Transmission Line Transformer Balun for Isolation-Enhanced Passive Mixer Design
Authors: He Ding , Yu Zhentao , Chen Jie , Du Kaiyuan , Zhu Zhiqiang , Cheng Pu , Tan Cheng
Published: February 2024

Generalized broadband operation facilitates multifunction or multiband highly integrated applications, such as modern transceiver systems, where ultra-wideband bidirectional passive mixers are favored to avoid a complex up/down-conversion scheme. In this paper, a modified Ruthroff-type transmission line transformer (TLT) balun is presented to enhance the isolation of the mixer from the local oscillator (LO) to the radio frequency (RF). Compared to the conventional methods, the proposed Ruthroff-type architecture adopts a combination of shunt capacitors and parallel coupled lines to improve the return loss at the LO port, thus effectively avoiding the area consumption for the diode-to-balun impedance transformation while simultaneously providing a suitable point for IF extraction. In addition, a parallel compensation technique consisting of an inductor and resistor is applied to the RF balun to significantly improve the amplitude/phase balance performance over a wide bandwidth. Benefiting from the aforementioned operations, an isolation-enhanced 8–30 GHz passive double-balanced mixer is designed as a proof-of-principle demonstration via 0.15-micrometer GaAs p-HEMT technology. It exhibits ultra-broadband performance with 7 dB average conversion loss and 50 dB LO-to-RF isolation under 15 dBm LO power. The monolithic microwave integrated circuit area is 0.96 × 1.68 mm2 including all pads.
Review of Droplet Printing Technologies for Flexible Electronic Devices: Materials, Control, and Applications
Authors: Jiang Jiaxin , Chen Xi , Mei Zexing , Chen Huatan , Chen Junyu , Wang Xiang , Li Shufan , Zhang Runyang , Zheng Gaofeng , Li Wenwang
Published: February 2024

Flexible devices have extensive applications in areas including wearable sensors, healthcare, smart packaging, energy, automotive and aerospace sectors, and other related fields. Droplet printing technology can be utilized to print flexible electronic components with micro/nanostructures on various scales, exhibiting good compatibility and wide material applicability for device production. This paper provides a comprehensive review of the current research status of droplet printing technologies and their applications across various domains, aiming to offer a valuable reference for researchers in related areas.
Refractive Index Modulation for Metal Electrodeposition-Based Active Smart Window Applications
Authors: Kim Hyojung , Kang Bong , Moon Cheon
Published: February 2024

One of the remarkable choices for active smart window technology is adopting a metal active layer via reversible metal electrodeposition (RME). As the metal layer efficiently blocks the solar energy gain, even a hundred-nanometer-thick scale, RME-based smart window has great attention. Recent developments are mainly focused on the various cases of electrolyte components and composition meeting technological standards. As metal nanostructures formed through the RME process involve plasmonic phenomena, advanced analysis, including plasmonic optics, which is beyond Beer–Lambert’s law, should be considered. However, as there is a lack of debates on the plasmonic optics applied to RME smart window technology, as research is mainly conducted through an exhaustive process. In this paper, in order to provide insight into the RME-based smart window development and alleviate the unclear part of plasmonic optics applied to the field, finite-difference time-domain electromagnetic simulations are conducted. In total, two extremely low-quality (Cr) and high-quality (Mg) plasmonic materials based on a nanoparticle array are considered as a metal medium. In addition, optical effects caused by the metal active layer, electrolyte, and nanoparticle embedment are investigated in detail. Overall simulations suggest that the effective refractive index is a decisive factor in the performance of RME-based smart windows.
Miniaturized Lens Antenna with Enhanced Gain and Dual-Focusing for Millimeter-Wave Radar System
Authors: Wang Jian , Duan Junping , Shen Xinxin , Wang Yongsheng , Zhang Binzhen
Published: February 2024

This paper presents a waveguide Lens antenna at the W-band adopting dual-focusing Lens to improve the performance. The Lens antenna consisted of a waveguide slotted structure and lenses processed using NOA73 meet the demands of miniaturization for current communication systems. The antenna radome fabricated using NOA73 not only protects the antenna structure but also improves the gain of the antenna by about 9.5 dBi via electromagnetic wave dual-focusing. A prototype is fabricated using novel UV-LIGA technology. Measured results are compared with simulated values. Measured results confirmed the fabricated antenna operated in the W-band with a 10 dB fractional bandwidth (FBW) of 6.5% from 97.5 to 104 GHz and a peak gain of 22 dBi at 100 GHz in the direction perpendicular to the plane of the feed waveguide. A good agreement between simulation and measurement is obtained, demonstrating efficient radiations in the operating band.
Assessment of Heavy Metal(loid) Pollution and Human Health Risks Associated with a Mineral (Zn, Cu, and Sn Ores) Processing Plant in Yunnan, Southwest China
Authors: Luo Wenping , Zhang Yan , Wei Pingtang , Sun Chengshuai
Published: February 2024

Understanding the contamination and sources of heavy metal(loid)s (HMs) at historical sites is vital for safeguarding human health and the ecological environment. This study focused on As, Hg, Cd, Cu, Pb, Ni, and Cr concentrations in the residual soil, groundwater, and surface water around a mineral processing plant. The sources of these elements and the human health risks posed by them were evaluated using various indexes. Soil HM concentrations exceeded background values for Yunnan Province, ranked as As > Pb > Cd > Cu > Hg > Ni. The river water met China’s Class II waterbody standard; however, Cd, Cu, Pb, and pH exceeded the maximum permissible sewage discharge concentrations in the accumulated water. The groundwater showed severe HM pollution, meeting China’s Class III water quality standard. The average value of the Nemerow pollution index was consistent with that of the single-factor pollution index in the following order: As > Pb > Cd > Ni > Cu > Hg. Children face heightened risk through the oral ingestion of As, Cd, and Pb, particularly in high-value sampling points in the residue deposit area. The main sources of these pollutants are anthropogenic activities and the soil formation matrix.
Effects of Chemical Short-Range Order and Temperature on Basic Structure Parameters and Stacking Fault Energies in Multi-Principal Element Alloys
Authors: Mubassira Subah , Jian Wu-Rong , Xu Shuozhi
Published: February 2024

In the realm of advanced material science, multi-principal element alloys (MPEAs) have emerged as a focal point due to their exceptional mechanical properties and adaptability for high-performance applications. This study embarks on an extensive investigation of four MPEAs—CoCrNi, MoNbTa, HfNbTaTiZr, and HfMoNbTaTi—alongside key pure metals (Mo, Nb, Ta, Ni) to unveil their structural and mechanical characteristics. Utilizing a blend of molecular statics and hybrid molecular dynamics/Monte Carlo simulations, the research delves into the impact of chemical short-range order (CSRO) and thermal effects on the fundamental structural parameters and stacking fault energies in these alloys. The study systematically analyzes quantities such as lattice parameters, elastic constants (C11, C12, and C44), and generalized stacking fault energies (GSFEs) across two distinct structures: random and CSRO. These properties are then evaluated at diverse temperatures (0, 300, 600, 900, 1200 K), offering a comprehensive understanding of temperature’s influence on material behavior. For CSRO, CoCrNi was annealed at 350 K and MoNbTa at 300 K, while both HfMoNbTaTi and HfNbTaTiZr were annealed at 300 K, 600 K, and 900 K, respectively. The results indicate that the lattice parameter increases with temperature, reflecting typical thermal expansion behavior. In contrast, both elastic constants and GSFE decrease with rising temperature, suggesting a reduction in resistance to stability and dislocation motion as thermal agitation intensifies. Notably, MPEAs with CSRO structures exhibit higher stiffness and GSFEs compared to their randomly structured counterparts, demonstrating the significant role of atomic ordering in enhancing material strength.